doc_id	sent_index	relation_id	relation	trigger	trigger_offset	arg_num	arg_head	arg_protein	arg_domain	arg_site	arg_sugar	arg_head_offset	arg_base_np	arg_base_np_offset	arg_np	arg_np_offset	sent_text
29296958	0	0	theme	endothelial	77:87	arg1	cells					89:93	endothelial cells	77:93	endothelial cells	77:93	Characterizing the O-glycosylation landscape of human plasma, platelets, and endothelial cells.
29296958	6	1	theme	protein	1442:1448	arg1	domains					1450:1456	protein domains	1442:1456	protein domains	1442:1456	Using an unbiased screen to identify associations between O-glycosites and protein annotations we found that O-glycans were over-represented close (± 15 amino acids) to tandem repeat regions, protease cleavage sites, within propeptides, and located on a select group of protein domains.
29296958	6	2	theme	protease	1364:1371	arg1	regions					1355:1361	tandem repeat regions	1341:1361	tandem repeat regions	1341:1361	Using an unbiased screen to identify associations between O-glycosites and protein annotations we found that O-glycans were over-represented close (± 15 amino acids) to tandem repeat regions, protease cleavage sites, within propeptides, and located on a select group of protein domains.
29296958	6	2	theme	protease	1364:1371	arg1	sites					1382:1386	protease cleavage sites	1364:1386	protease cleavage sites	1364:1386	Using an unbiased screen to identify associations between O-glycosites and protein annotations we found that O-glycans were over-represented close (± 15 amino acids) to tandem repeat regions, protease cleavage sites, within propeptides, and located on a select group of protein domains.
29296958	6	3	theme	cleavage	1373:1380	arg1	regions					1355:1361	tandem repeat regions	1341:1361	tandem repeat regions	1341:1361	Using an unbiased screen to identify associations between O-glycosites and protein annotations we found that O-glycans were over-represented close (± 15 amino acids) to tandem repeat regions, protease cleavage sites, within propeptides, and located on a select group of protein domains.
29296958	6	3	theme	cleavage	1373:1380	arg1	sites					1382:1386	protease cleavage sites	1364:1386	protease cleavage sites	1364:1386	Using an unbiased screen to identify associations between O-glycosites and protein annotations we found that O-glycans were over-represented close (± 15 amino acids) to tandem repeat regions, protease cleavage sites, within propeptides, and located on a select group of protein domains.
29296958	3	4	theme	affinity	515:522	arg1	chromatography					524:537	lectin affinity chromatography	508:537	lectin affinity chromatography coupled to liquid chromatography mass spectrometry (LC-MS)	508:596	Here we performed the first systematic analysis of native-O-glycosylation using lectin affinity chromatography coupled to liquid chromatography mass spectrometry (LC-MS)/MS to determine the precise location of O-glycans in human plasma, platelets, and endothelial cells, which coordinately regulate hemostasis.
29296958	8	5	theme	O-glycosylation	1755:1769	arg1	properties					1734:1743	the global properties	1723:1743	the global properties of native O-glycosylation	1723:1769	Collectively, these data illustrate the global properties of native O-glycosylation and provide the requisite roadmap for future biomarker and structure-function studies.
29296958	7	6	theme	O-glycosites	1477:1488	arg1	importance					1463:1472	The importance	1459:1472	The importance of O-glycosites in proximity to proteolytic cleavage sites	1459:1531	The importance of O-glycosites in proximity to proteolytic cleavage sites was further supported by in vitro peptide assays demonstrating that proteolysis of key hemostatic proteins can be inhibited by the presence of O-glycans.
29296958	5	7	theme	cell-dependent	1149:1162	arg1	manner					1164:1169	a cell-dependent manner	1147:1169	a cell-dependent manner	1147:1169	Investigation of the general properties of O-glycosylation established that it is a heterogeneous modification, frequently occurring at low density within disordered regions in a cell-dependent manner.
29296958	6	8	dep	close	1313:1317	arg1	acids					1331:1335	± 15 amino acids	1320:1335	± 15 amino acids	1320:1335	Using an unbiased screen to identify associations between O-glycosites and protein annotations we found that O-glycans were over-represented close (± 15 amino acids) to tandem repeat regions, protease cleavage sites, within propeptides, and located on a select group of protein domains.
29296958	5	9	theme	heterogeneous	1054:1066	arg1	modification					1068:1079	a heterogeneous modification	1052:1079	a heterogeneous modification	1052:1079	Investigation of the general properties of O-glycosylation established that it is a heterogeneous modification, frequently occurring at low density within disordered regions in a cell-dependent manner.
29296958	5	9	theme	heterogeneous	1054:1066	arg1	it					1046:1047	it	1046:1047	it	1046:1047	Investigation of the general properties of O-glycosylation established that it is a heterogeneous modification, frequently occurring at low density within disordered regions in a cell-dependent manner.
29296958	4	10	theme	ubiquitous	919:928	arg1	modification					930:941	a ubiquitous modification	917:941	a ubiquitous modification of extracellular proteins	917:967	We identified the hitherto largest O-glycoproteome from native tissue with a total of 649 glycoproteins and 1123 nonambiguous O-glycosites, demonstrating that O-glycosylation is a ubiquitous modification of extracellular proteins.
29296958	4	10	theme	ubiquitous	919:928	arg1	O-glycosylation					898:912	O-glycosylation	898:912	O-glycosylation	898:912	We identified the hitherto largest O-glycoproteome from native tissue with a total of 649 glycoproteins and 1123 nonambiguous O-glycosites, demonstrating that O-glycosylation is a ubiquitous modification of extracellular proteins.
29296958	4	11	from	tissue	802:807	arg1	O-glycoproteome					774:788	the hitherto largest O-glycoproteome	753:788	the hitherto largest O-glycoproteome from native tissue	753:807	We identified the hitherto largest O-glycoproteome from native tissue with a total of 649 glycoproteins and 1123 nonambiguous O-glycosites, demonstrating that O-glycosylation is a ubiquitous modification of extracellular proteins.
29296958	3	12	theme	mass	572:575	arg1	LC-MS					591:595	LC-MS	591:595	LC-MS	591:595	Here we performed the first systematic analysis of native-O-glycosylation using lectin affinity chromatography coupled to liquid chromatography mass spectrometry (LC-MS)/MS to determine the precise location of O-glycans in human plasma, platelets, and endothelial cells, which coordinately regulate hemostasis.
29296958	3	12	theme	mass	572:575	arg1	spectrometry					577:588	liquid chromatography mass spectrometry	550:588	liquid chromatography mass spectrometry (LC-MS)	550:596	Here we performed the first systematic analysis of native-O-glycosylation using lectin affinity chromatography coupled to liquid chromatography mass spectrometry (LC-MS)/MS to determine the precise location of O-glycans in human plasma, platelets, and endothelial cells, which coordinately regulate hemostasis.
29296958	4	13	theme	largest	766:772	arg1	O-glycoproteome					774:788	the hitherto largest O-glycoproteome	753:788	the hitherto largest O-glycoproteome from native tissue	753:807	We identified the hitherto largest O-glycoproteome from native tissue with a total of 649 glycoproteins and 1123 nonambiguous O-glycosites, demonstrating that O-glycosylation is a ubiquitous modification of extracellular proteins.
29296958	7	14	theme	proteins	1631:1638	arg1	proteolysis					1601:1611	proteolysis	1601:1611	proteolysis of key hemostatic proteins	1601:1638	The importance of O-glycosites in proximity to proteolytic cleavage sites was further supported by in vitro peptide assays demonstrating that proteolysis of key hemostatic proteins can be inhibited by the presence of O-glycans.
29296958	0	15	theme	cells	89:93	arg1	landscape					35:43	the O-glycosylation landscape	15:43	the O-glycosylation landscape of human plasma, platelets, and endothelial cells	15:93	Characterizing the O-glycosylation landscape of human plasma, platelets, and endothelial cells.
29296958	6	16	theme	repeat	1348:1353	arg1	regions					1355:1361	tandem repeat regions	1341:1361	tandem repeat regions	1341:1361	Using an unbiased screen to identify associations between O-glycosites and protein annotations we found that O-glycans were over-represented close (± 15 amino acids) to tandem repeat regions, protease cleavage sites, within propeptides, and located on a select group of protein domains.
29296958	6	16	theme	repeat	1348:1353	arg1	sites					1382:1386	protease cleavage sites	1364:1386	protease cleavage sites	1364:1386	Using an unbiased screen to identify associations between O-glycosites and protein annotations we found that O-glycans were over-represented close (± 15 amino acids) to tandem repeat regions, protease cleavage sites, within propeptides, and located on a select group of protein domains.
29296958	2	17	theme	important	283:291	arg1	roles					293:297	important roles	283:297	important roles	283:297	Multiple studies indicate that glycans play important roles in the hemostatic system; however, most investigations have focused on N-glycans because of the complexity of O-glycan analysis.
29296958	3	18	from	location	626:633	arg1	platelets					665:673	platelets	665:673	platelets	665:673	Here we performed the first systematic analysis of native-O-glycosylation using lectin affinity chromatography coupled to liquid chromatography mass spectrometry (LC-MS)/MS to determine the precise location of O-glycans in human plasma, platelets, and endothelial cells, which coordinately regulate hemostasis.
29296958	3	18	from	location	626:633	arg1	plasma					657:662	human plasma	651:662	human plasma	651:662	Here we performed the first systematic analysis of native-O-glycosylation using lectin affinity chromatography coupled to liquid chromatography mass spectrometry (LC-MS)/MS to determine the precise location of O-glycans in human plasma, platelets, and endothelial cells, which coordinately regulate hemostasis.
29296958	3	18	from	location	626:633	arg1	cells					692:696	endothelial cells	680:696	endothelial cells	680:696	Here we performed the first systematic analysis of native-O-glycosylation using lectin affinity chromatography coupled to liquid chromatography mass spectrometry (LC-MS)/MS to determine the precise location of O-glycans in human plasma, platelets, and endothelial cells, which coordinately regulate hemostasis.
29296958	5	19	theme	properties	999:1008	arg1	Investigation					970:982	Investigation	970:982	Investigation of the general properties of O-glycosylation	970:1027	Investigation of the general properties of O-glycosylation established that it is a heterogeneous modification, frequently occurring at low density within disordered regions in a cell-dependent manner.
29296958	3	20	theme	liquid	550:555	arg1	LC-MS					591:595	LC-MS	591:595	LC-MS	591:595	Here we performed the first systematic analysis of native-O-glycosylation using lectin affinity chromatography coupled to liquid chromatography mass spectrometry (LC-MS)/MS to determine the precise location of O-glycans in human plasma, platelets, and endothelial cells, which coordinately regulate hemostasis.
29296958	3	20	theme	liquid	550:555	arg1	spectrometry					577:588	liquid chromatography mass spectrometry	550:588	liquid chromatography mass spectrometry (LC-MS)	550:596	Here we performed the first systematic analysis of native-O-glycosylation using lectin affinity chromatography coupled to liquid chromatography mass spectrometry (LC-MS)/MS to determine the precise location of O-glycans in human plasma, platelets, and endothelial cells, which coordinately regulate hemostasis.
29296958	8	21	theme	requisite	1787:1795	arg1	roadmap					1797:1803	the requisite roadmap	1783:1803	the requisite roadmap for future biomarker and structure-function studies	1783:1855	Collectively, these data illustrate the global properties of native O-glycosylation and provide the requisite roadmap for future biomarker and structure-function studies.
29296958	6	22	dep	O-glycosites	1230:1241	arg1	annotations					1255:1265	annotations	1255:1265	annotations	1255:1265	Using an unbiased screen to identify associations between O-glycosites and protein annotations we found that O-glycans were over-represented close (± 15 amino acids) to tandem repeat regions, protease cleavage sites, within propeptides, and located on a select group of protein domains.
29296958	7	23	theme	cleavage	1518:1525	arg1	sites					1527:1531	proteolytic cleavage sites	1506:1531	proteolytic cleavage sites	1506:1531	The importance of O-glycosites in proximity to proteolytic cleavage sites was further supported by in vitro peptide assays demonstrating that proteolysis of key hemostatic proteins can be inhibited by the presence of O-glycans.
29296958	4	24	theme	O-glycosites	865:876	arg1	total					816:820	a total	814:820	a total of 649 glycoproteins and 1123 nonambiguous O-glycosites	814:876	We identified the hitherto largest O-glycoproteome from native tissue with a total of 649 glycoproteins and 1123 nonambiguous O-glycosites, demonstrating that O-glycosylation is a ubiquitous modification of extracellular proteins.
29296958	3	25	theme	chromatography	557:570	arg1	LC-MS					591:595	LC-MS	591:595	LC-MS	591:595	Here we performed the first systematic analysis of native-O-glycosylation using lectin affinity chromatography coupled to liquid chromatography mass spectrometry (LC-MS)/MS to determine the precise location of O-glycans in human plasma, platelets, and endothelial cells, which coordinately regulate hemostasis.
29296958	3	25	theme	chromatography	557:570	arg1	spectrometry					577:588	liquid chromatography mass spectrometry	550:588	liquid chromatography mass spectrometry (LC-MS)	550:596	Here we performed the first systematic analysis of native-O-glycosylation using lectin affinity chromatography coupled to liquid chromatography mass spectrometry (LC-MS)/MS to determine the precise location of O-glycans in human plasma, platelets, and endothelial cells, which coordinately regulate hemostasis.
29296958	8	26	theme	future	1809:1814	arg1	biomarker					1816:1824	future biomarker and structure-function studies	1809:1855	biomarker	1816:1824	Collectively, these data illustrate the global properties of native O-glycosylation and provide the requisite roadmap for future biomarker and structure-function studies.
29296958	4	27	gly	glycoproteins	829:841	arg1	glycoproteins					829:841	649 glycoproteins	825:841	649 glycoproteins	825:841	We identified the hitherto largest O-glycoproteome from native tissue with a total of 649 glycoproteins and 1123 nonambiguous O-glycosites, demonstrating that O-glycosylation is a ubiquitous modification of extracellular proteins.
29296958	5	28	theme	general	991:997	arg1	properties					999:1008	the general properties	987:1008	the general properties of O-glycosylation	987:1027	Investigation of the general properties of O-glycosylation established that it is a heterogeneous modification, frequently occurring at low density within disordered regions in a cell-dependent manner.
29296958	6	29	dep	screen	1190:1195	arg1	identify					1200:1207	identify	1200:1207	to identify associations between O-glycosites and protein annotations	1197:1265	Using an unbiased screen to identify associations between O-glycosites and protein annotations we found that O-glycans were over-represented close (± 15 amino acids) to tandem repeat regions, protease cleavage sites, within propeptides, and located on a select group of protein domains.
29296958	6	30	theme	tandem	1341:1346	arg1	regions					1355:1361	tandem repeat regions	1341:1361	tandem repeat regions	1341:1361	Using an unbiased screen to identify associations between O-glycosites and protein annotations we found that O-glycans were over-represented close (± 15 amino acids) to tandem repeat regions, protease cleavage sites, within propeptides, and located on a select group of protein domains.
29296958	6	30	theme	tandem	1341:1346	arg1	sites					1382:1386	protease cleavage sites	1364:1386	protease cleavage sites	1364:1386	Using an unbiased screen to identify associations between O-glycosites and protein annotations we found that O-glycans were over-represented close (± 15 amino acids) to tandem repeat regions, protease cleavage sites, within propeptides, and located on a select group of protein domains.
29296958	2	31	theme	hemostatic	306:315	arg1	system					317:322	the hemostatic system	302:322	the hemostatic system	302:322	Multiple studies indicate that glycans play important roles in the hemostatic system; however, most investigations have focused on N-glycans because of the complexity of O-glycan analysis.
29296958	6	32	gly	O-glycosites	1230:1241	arg2	O-glycosites					1230:1241	O-glycosites	1230:1241	O-glycosites	1230:1241	Using an unbiased screen to identify associations between O-glycosites and protein annotations we found that O-glycans were over-represented close (± 15 amino acids) to tandem repeat regions, protease cleavage sites, within propeptides, and located on a select group of protein domains.
29296958	6	33	dep	found	1270:1274	arg1	Using					1172:1176	Using	1172:1176	Using an unbiased screen to identify associations between O-glycosites and protein annotations	1172:1265	Using an unbiased screen to identify associations between O-glycosites and protein annotations we found that O-glycans were over-represented close (± 15 amino acids) to tandem repeat regions, protease cleavage sites, within propeptides, and located on a select group of protein domains.
29296958	2	34	theme	analysis	418:425	arg1	complexity					395:404	the complexity	391:404	the complexity of O-glycan analysis	391:425	Multiple studies indicate that glycans play important roles in the hemostatic system; however, most investigations have focused on N-glycans because of the complexity of O-glycan analysis.
29296958	0	35	theme	O-glycosylation	19:33	arg1	landscape					35:43	the O-glycosylation landscape	15:43	the O-glycosylation landscape of human plasma, platelets, and endothelial cells	15:93	Characterizing the O-glycosylation landscape of human plasma, platelets, and endothelial cells.
29296958	7	36	dep	in	1558:1559	arg1	vitro					1561:1565	vitro	1561:1565	vitro	1561:1565	The importance of O-glycosites in proximity to proteolytic cleavage sites was further supported by in vitro peptide assays demonstrating that proteolysis of key hemostatic proteins can be inhibited by the presence of O-glycans.
29296958	4	37	gly	O-glycosites	865:876	arg2	O-glycosites					865:876	1123 nonambiguous O-glycosites	847:876	1123 nonambiguous O-glycosites	847:876	We identified the hitherto largest O-glycoproteome from native tissue with a total of 649 glycoproteins and 1123 nonambiguous O-glycosites, demonstrating that O-glycosylation is a ubiquitous modification of extracellular proteins.
29296958	7	38	theme	key	1616:1618	arg1	proteins					1631:1638	key hemostatic proteins	1616:1638	key hemostatic proteins	1616:1638	The importance of O-glycosites in proximity to proteolytic cleavage sites was further supported by in vitro peptide assays demonstrating that proteolysis of key hemostatic proteins can be inhibited by the presence of O-glycans.
29296958	1	39	theme	hemostatic	100:109	arg1	system					111:116	The hemostatic system	96:116	The hemostatic system	96:116	The hemostatic system comprises platelet aggregation, coagulation, and fibrinolysis, and is critical to the maintenance of vascular integrity.
29296958	1	39	theme	hemostatic	100:109	arg1	critical					188:195	critical	188:195	critical	188:195	The hemostatic system comprises platelet aggregation, coagulation, and fibrinolysis, and is critical to the maintenance of vascular integrity.
29296958	2	40	theme	O-glycan	409:416	arg1	analysis					418:425	O-glycan analysis	409:425	O-glycan analysis	409:425	Multiple studies indicate that glycans play important roles in the hemostatic system; however, most investigations have focused on N-glycans because of the complexity of O-glycan analysis.
29296958	0	41	theme	human	48:52	arg1	plasma					54:59	human plasma	48:59	human plasma	48:59	Characterizing the O-glycosylation landscape of human plasma, platelets, and endothelial cells.
29296958	6	42	theme	±	1320:1320	arg1	acids					1331:1335	± 15 amino acids	1320:1335	± 15 amino acids	1320:1335	Using an unbiased screen to identify associations between O-glycosites and protein annotations we found that O-glycans were over-represented close (± 15 amino acids) to tandem repeat regions, protease cleavage sites, within propeptides, and located on a select group of protein domains.
29296958	7	43	theme	hemostatic	1620:1629	arg1	proteins					1631:1638	key hemostatic proteins	1616:1638	key hemostatic proteins	1616:1638	The importance of O-glycosites in proximity to proteolytic cleavage sites was further supported by in vitro peptide assays demonstrating that proteolysis of key hemostatic proteins can be inhibited by the presence of O-glycans.
29296958	6	44	theme	amino	1325:1329	arg1	acids					1331:1335	± 15 amino acids	1320:1335	± 15 amino acids	1320:1335	Using an unbiased screen to identify associations between O-glycosites and protein annotations we found that O-glycans were over-represented close (± 15 amino acids) to tandem repeat regions, protease cleavage sites, within propeptides, and located on a select group of protein domains.
29296958	8	45	theme	structure-function	1830:1847	arg1	studies					1849:1855	future biomarker and structure-function studies	1809:1855	studies	1849:1855	Collectively, these data illustrate the global properties of native O-glycosylation and provide the requisite roadmap for future biomarker and structure-function studies.
29296958	7	46	theme	proteolytic	1506:1516	arg1	sites					1527:1531	proteolytic cleavage sites	1506:1531	proteolytic cleavage sites	1506:1531	The importance of O-glycosites in proximity to proteolytic cleavage sites was further supported by in vitro peptide assays demonstrating that proteolysis of key hemostatic proteins can be inhibited by the presence of O-glycans.
29296958	3	47	theme	human	651:655	arg1	plasma					657:662	human plasma	651:662	human plasma	651:662	Here we performed the first systematic analysis of native-O-glycosylation using lectin affinity chromatography coupled to liquid chromatography mass spectrometry (LC-MS)/MS to determine the precise location of O-glycans in human plasma, platelets, and endothelial cells, which coordinately regulate hemostasis.
29296958	3	48	theme	precise	618:624	arg1	location					626:633	the precise location	614:633	the precise location of O-glycans in human plasma, platelets, and endothelial cells, which coordinately regulate hemostasis	614:736	Here we performed the first systematic analysis of native-O-glycosylation using lectin affinity chromatography coupled to liquid chromatography mass spectrometry (LC-MS)/MS to determine the precise location of O-glycans in human plasma, platelets, and endothelial cells, which coordinately regulate hemostasis.
29296958	2	49	theme	complexity	395:404	arg1	N-glycans					370:378	N-glycans	370:378	N-glycans because of the complexity of O-glycan analysis	370:425	Multiple studies indicate that glycans play important roles in the hemostatic system; however, most investigations have focused on N-glycans because of the complexity of O-glycan analysis.
29296958	6	50	theme	domains	1450:1456	arg1	group					1433:1437	a select group	1424:1437	a select group of protein domains	1424:1456	Using an unbiased screen to identify associations between O-glycosites and protein annotations we found that O-glycans were over-represented close (± 15 amino acids) to tandem repeat regions, protease cleavage sites, within propeptides, and located on a select group of protein domains.
29296958	6	50	theme	domains	1450:1456	arg1	domains					1450:1456	protein domains	1442:1456	protein domains	1442:1456	Using an unbiased screen to identify associations between O-glycosites and protein annotations we found that O-glycans were over-represented close (± 15 amino acids) to tandem repeat regions, protease cleavage sites, within propeptides, and located on a select group of protein domains.
29296958	4	51	theme	nonambiguous	852:863	arg1	O-glycosites					865:876	1123 nonambiguous O-glycosites	847:876	1123 nonambiguous O-glycosites	847:876	We identified the hitherto largest O-glycoproteome from native tissue with a total of 649 glycoproteins and 1123 nonambiguous O-glycosites, demonstrating that O-glycosylation is a ubiquitous modification of extracellular proteins.
29296958	6	52	theme	unbiased	1181:1188	arg1	screen					1190:1195	an unbiased screen	1178:1195	an unbiased screen to identify associations between O-glycosites and protein annotations	1178:1265	Using an unbiased screen to identify associations between O-glycosites and protein annotations we found that O-glycans were over-represented close (± 15 amino acids) to tandem repeat regions, protease cleavage sites, within propeptides, and located on a select group of protein domains.
29296958	2	53	theme	most	334:337	arg1	investigations					339:352	most investigations	334:352	most investigations	334:352	Multiple studies indicate that glycans play important roles in the hemostatic system; however, most investigations have focused on N-glycans because of the complexity of O-glycan analysis.
29296958	0	54	theme	plasma	54:59	arg1	landscape					35:43	the O-glycosylation landscape	15:43	the O-glycosylation landscape of human plasma, platelets, and endothelial cells	15:93	Characterizing the O-glycosylation landscape of human plasma, platelets, and endothelial cells.
29296958	7	55	theme	O-glycans	1676:1684	arg1	presence					1664:1671	the presence	1660:1671	the presence of O-glycans	1660:1684	The importance of O-glycosites in proximity to proteolytic cleavage sites was further supported by in vitro peptide assays demonstrating that proteolysis of key hemostatic proteins can be inhibited by the presence of O-glycans.
29296958	3	56	theme	endothelial	680:690	arg1	cells					692:696	endothelial cells	680:696	endothelial cells	680:696	Here we performed the first systematic analysis of native-O-glycosylation using lectin affinity chromatography coupled to liquid chromatography mass spectrometry (LC-MS)/MS to determine the precise location of O-glycans in human plasma, platelets, and endothelial cells, which coordinately regulate hemostasis.
29296958	5	57	theme	low	1106:1108	arg1	density					1110:1116	low density	1106:1116	low density	1106:1116	Investigation of the general properties of O-glycosylation established that it is a heterogeneous modification, frequently occurring at low density within disordered regions in a cell-dependent manner.
29296958	3	58	theme	lectin	508:513	arg1	chromatography					524:537	lectin affinity chromatography	508:537	lectin affinity chromatography coupled to liquid chromatography mass spectrometry (LC-MS)	508:596	Here we performed the first systematic analysis of native-O-glycosylation using lectin affinity chromatography coupled to liquid chromatography mass spectrometry (LC-MS)/MS to determine the precise location of O-glycans in human plasma, platelets, and endothelial cells, which coordinately regulate hemostasis.
29296958	4	59	theme	proteins	960:967	arg1	modification					930:941	a ubiquitous modification	917:941	a ubiquitous modification of extracellular proteins	917:967	We identified the hitherto largest O-glycoproteome from native tissue with a total of 649 glycoproteins and 1123 nonambiguous O-glycosites, demonstrating that O-glycosylation is a ubiquitous modification of extracellular proteins.
29296958	4	59	theme	proteins	960:967	arg1	O-glycosylation					898:912	O-glycosylation	898:912	O-glycosylation	898:912	We identified the hitherto largest O-glycoproteome from native tissue with a total of 649 glycoproteins and 1123 nonambiguous O-glycosites, demonstrating that O-glycosylation is a ubiquitous modification of extracellular proteins.
29296958	7	60	theme	peptide	1567:1573	arg1	assays					1575:1580	in vitro peptide assays	1558:1580	in vitro peptide assays demonstrating that proteolysis of key hemostatic proteins can be inhibited by the presence of O-glycans	1558:1684	The importance of O-glycosites in proximity to proteolytic cleavage sites was further supported by in vitro peptide assays demonstrating that proteolysis of key hemostatic proteins can be inhibited by the presence of O-glycans.
29296958	3	61	theme	O-glycans	638:646	arg1	location					626:633	the precise location	614:633	the precise location of O-glycans in human plasma, platelets, and endothelial cells, which coordinately regulate hemostasis	614:736	Here we performed the first systematic analysis of native-O-glycosylation using lectin affinity chromatography coupled to liquid chromatography mass spectrometry (LC-MS)/MS to determine the precise location of O-glycans in human plasma, platelets, and endothelial cells, which coordinately regulate hemostasis.
29296958	4	62	theme	glycoproteins	829:841	arg1	total					816:820	a total	814:820	a total of 649 glycoproteins and 1123 nonambiguous O-glycosites	814:876	We identified the hitherto largest O-glycoproteome from native tissue with a total of 649 glycoproteins and 1123 nonambiguous O-glycosites, demonstrating that O-glycosylation is a ubiquitous modification of extracellular proteins.
29296958	1	63	theme	platelet	128:135	arg1	aggregation					137:147	platelet aggregation	128:147	platelet aggregation	128:147	The hemostatic system comprises platelet aggregation, coagulation, and fibrinolysis, and is critical to the maintenance of vascular integrity.
29296958	7	64	from	importance	1463:1472	arg1	proximity					1493:1501	proximity	1493:1501	proximity to proteolytic cleavage sites	1493:1531	The importance of O-glycosites in proximity to proteolytic cleavage sites was further supported by in vitro peptide assays demonstrating that proteolysis of key hemostatic proteins can be inhibited by the presence of O-glycans.
29296958	4	65	theme	extracellular	946:958	arg1	proteins					960:967	extracellular proteins	946:967	extracellular proteins	946:967	We identified the hitherto largest O-glycoproteome from native tissue with a total of 649 glycoproteins and 1123 nonambiguous O-glycosites, demonstrating that O-glycosylation is a ubiquitous modification of extracellular proteins.
29296958	5	66	theme	disordered	1125:1134	arg1	regions					1136:1142	disordered regions	1125:1142	disordered regions	1125:1142	Investigation of the general properties of O-glycosylation established that it is a heterogeneous modification, frequently occurring at low density within disordered regions in a cell-dependent manner.
29296958	4	67	theme	native	795:800	arg1	tissue					802:807	native tissue	795:807	native tissue	795:807	We identified the hitherto largest O-glycoproteome from native tissue with a total of 649 glycoproteins and 1123 nonambiguous O-glycosites, demonstrating that O-glycosylation is a ubiquitous modification of extracellular proteins.
29296958	3	68	theme	native-O-glycosylation	479:500	arg1	analysis					467:474	the first systematic analysis	446:474	the first systematic analysis of native-O-glycosylation	446:500	Here we performed the first systematic analysis of native-O-glycosylation using lectin affinity chromatography coupled to liquid chromatography mass spectrometry (LC-MS)/MS to determine the precise location of O-glycans in human plasma, platelets, and endothelial cells, which coordinately regulate hemostasis.
29296958	8	69	theme	native	1748:1753	arg1	O-glycosylation					1755:1769	native O-glycosylation	1748:1769	native O-glycosylation	1748:1769	Collectively, these data illustrate the global properties of native O-glycosylation and provide the requisite roadmap for future biomarker and structure-function studies.
29296958	1	70	theme	vascular	219:226	arg1	integrity					228:236	vascular integrity	219:236	vascular integrity	219:236	The hemostatic system comprises platelet aggregation, coagulation, and fibrinolysis, and is critical to the maintenance of vascular integrity.
29296958	7	71	gly	O-glycosites	1477:1488	arg2	O-glycosites					1477:1488	O-glycosites	1477:1488	O-glycosites	1477:1488	The importance of O-glycosites in proximity to proteolytic cleavage sites was further supported by in vitro peptide assays demonstrating that proteolysis of key hemostatic proteins can be inhibited by the presence of O-glycans.
29296958	3	72	theme	first	450:454	arg1	analysis					467:474	the first systematic analysis	446:474	the first systematic analysis of native-O-glycosylation	446:500	Here we performed the first systematic analysis of native-O-glycosylation using lectin affinity chromatography coupled to liquid chromatography mass spectrometry (LC-MS)/MS to determine the precise location of O-glycans in human plasma, platelets, and endothelial cells, which coordinately regulate hemostasis.
29296958	3	73	theme	systematic	456:465	arg1	analysis					467:474	the first systematic analysis	446:474	the first systematic analysis of native-O-glycosylation	446:500	Here we performed the first systematic analysis of native-O-glycosylation using lectin affinity chromatography coupled to liquid chromatography mass spectrometry (LC-MS)/MS to determine the precise location of O-glycans in human plasma, platelets, and endothelial cells, which coordinately regulate hemostasis.
29296958	5	74	theme	O-glycosylation	1013:1027	arg1	properties					999:1008	the general properties	987:1008	the general properties of O-glycosylation	987:1027	Investigation of the general properties of O-glycosylation established that it is a heterogeneous modification, frequently occurring at low density within disordered regions in a cell-dependent manner.
29296958	8	75	theme	global	1727:1732	arg1	properties					1734:1743	the global properties	1723:1743	the global properties of native O-glycosylation	1723:1769	Collectively, these data illustrate the global properties of native O-glycosylation and provide the requisite roadmap for future biomarker and structure-function studies.
29296958	1	76	theme	integrity	228:236	arg1	maintenance					204:214	the maintenance	200:214	the maintenance of vascular integrity	200:236	The hemostatic system comprises platelet aggregation, coagulation, and fibrinolysis, and is critical to the maintenance of vascular integrity.
29296958	0	77	theme	platelets	62:70	arg1	landscape					35:43	the O-glycosylation landscape	15:43	the O-glycosylation landscape of human plasma, platelets, and endothelial cells	15:93	Characterizing the O-glycosylation landscape of human plasma, platelets, and endothelial cells.
29296958	7	78	theme	in	1558:1559	arg1	assays					1575:1580	in vitro peptide assays	1558:1580	in vitro peptide assays demonstrating that proteolysis of key hemostatic proteins can be inhibited by the presence of O-glycans	1558:1684	The importance of O-glycosites in proximity to proteolytic cleavage sites was further supported by in vitro peptide assays demonstrating that proteolysis of key hemostatic proteins can be inhibited by the presence of O-glycans.
29296958	2	79	theme	Multiple	239:246	arg1	studies					248:254	Multiple studies	239:254	Multiple studies	239:254	Multiple studies indicate that glycans play important roles in the hemostatic system; however, most investigations have focused on N-glycans because of the complexity of O-glycan analysis.
29296958	6	80	theme	select	1426:1431	arg1	group					1433:1437	a select group	1424:1437	a select group of protein domains	1424:1456	Using an unbiased screen to identify associations between O-glycosites and protein annotations we found that O-glycans were over-represented close (± 15 amino acids) to tandem repeat regions, protease cleavage sites, within propeptides, and located on a select group of protein domains.
29296958	6	80	theme	select	1426:1431	arg1	domains					1450:1456	protein domains	1442:1456	protein domains	1442:1456	Using an unbiased screen to identify associations between O-glycosites and protein annotations we found that O-glycans were over-represented close (± 15 amino acids) to tandem repeat regions, protease cleavage sites, within propeptides, and located on a select group of protein domains.
25211026	11	0	theme	PrP	1667:1669	arg1	status					1657:1662	the sialylation status	1641:1662	the sialylation status of PrP(C)	1641:1672	Surprisingly, the sialylation status of PrP(C) was also found to control PrP(Sc) glycoform ratio.
25211026	13	1	theme	distribution	1863:1874	arg1	analysis					1844:1851	2D analysis	1841:1851	2D analysis of charge distribution	1841:1874	2D analysis of charge distribution revealed that the sialylation status of brain-derived PrP(C) differed from that of spleen-derived PrP(C).
25211026	10	2	theme	homologous	1580:1589	arg1	replication					1614:1624	homologous and heterologous prion replication	1580:1624	homologous and heterologous prion replication	1580:1624	These results suggest that the negative charge of sialic acid controls the energy barrier of homologous and heterologous prion replication.
25211026	10	3	theme	sialic	1537:1542	arg1	acid					1544:1547	sialic acid	1537:1547	sialic acid	1537:1547	These results suggest that the negative charge of sialic acid controls the energy barrier of homologous and heterologous prion replication.
25211026	1	4	theme	prion	253:257	arg1	protein					259:265	the prion protein	249:265	the prion protein (PrP(C))	249:274	The central event underlying prion diseases involves conformational change of the cellular form of the prion protein (PrP(C)) into the disease-associated, transmissible form (PrP(Sc)).
25211026	12	5	theme	diglycosylated	1804:1817	arg1	glycoform					1819:1827	the diglycosylated glycoform	1800:1827	the diglycosylated glycoform in PrP(Sc)	1800:1838	A decrease in Pr(PC) sialylation levels resulted in a higher percentage of the diglycosylated glycoform in PrP(Sc).
25211026	4	6	theme	replication	739:749	arg1	rate					751:754	prion replication rate	733:754	prion replication rate	733:754	The current work highlights the previously unappreciated role of sialylation of PrP(C) glycans in prion pathogenesis, including its role in controlling prion replication rate, infectivity, cross-species barrier and PrP(Sc) glycoform ratio.
25211026	6	7	theme	PMCAb-derived	1024:1036	arg1	Sc					1042:1043	Sc	1042:1043	Sc	1042:1043	As a result, PMCAb-derived PrP(Sc) was less sialylated than brain-derived PrP(Sc).
25211026	6	7	theme	PMCAb-derived	1024:1036	arg1	result					1016:1021	a result	1014:1021	a result	1014:1021	As a result, PMCAb-derived PrP(Sc) was less sialylated than brain-derived PrP(Sc).
25211026	6	7	theme	PMCAb-derived	1024:1036	arg1	PrP					1038:1040	PMCAb-derived PrP	1024:1040	PMCAb-derived PrP(Sc)	1024:1044	As a result, PMCAb-derived PrP(Sc) was less sialylated than brain-derived PrP(Sc).
25211026	13	8	theme	charge	1856:1861	arg1	distribution					1863:1874	charge distribution	1856:1874	charge distribution	1856:1874	2D analysis of charge distribution revealed that the sialylation status of brain-derived PrP(C) differed from that of spleen-derived PrP(C).
25211026	2	9	contain	contains	370:377	arg1	sialoglycoprotein					347:363	a sialoglycoprotein	345:363	a sialoglycoprotein that contains two conserved N-glycosylation sites	345:413	Pr(PC) is a sialoglycoprotein that contains two conserved N-glycosylation sites.
25211026	2	9	contain	contains	370:377	arg2	sites					409:413	two conserved N-glycosylation sites	379:413	two conserved N-glycosylation sites	379:413	Pr(PC) is a sialoglycoprotein that contains two conserved N-glycosylation sites.
25211026	2	9	contain	contains	370:377	arg1	Pr					335:336	Pr	335:336	Pr(PC)	335:340	Pr(PC) is a sialoglycoprotein that contains two conserved N-glycosylation sites.
25211026	1	10	theme	central	154:160	arg1	event					162:166	The central event	150:166	The central event underlying prion diseases	150:192	The central event underlying prion diseases involves conformational change of the cellular form of the prion protein (PrP(C)) into the disease-associated, transmissible form (PrP(Sc)).
25211026	7	11	from	decrease	1096:1103	arg1	sialylation					1114:1124	PrPSc sialylation	1108:1124	PrPSc sialylation	1108:1124	A decrease in PrPSc sialylation correlated with a drop in infectivity of PMCAb-derived material.
25211026	3	12	theme	amino	502:506	arg1	sequence					513:520	amino acid sequence	502:520	amino acid sequence of host PrP(C)	502:535	Among the key parameters that control prion replication identified over the years are amino acid sequence of host PrP(C) and the strain-specific structure of PrPSc.
25211026	5	13	from	amplification	905:917	arg1	Misfolding					930:939	Protein Misfolding Cyclic Amplification (PMCAb)	922:968	Protein Misfolding Cyclic Amplification (PMCAb)	922:968	The current study demonstrates that undersialylated PrP(C) is selected during prion amplification in Protein Misfolding Cyclic Amplification (PMCAb) at the expense of oversialylated PrP(C).
25211026	4	14	theme	glycoform	804:812	arg1	ratio					814:818	glycoform ratio	804:818	glycoform ratio	804:818	The current work highlights the previously unappreciated role of sialylation of PrP(C) glycans in prion pathogenesis, including its role in controlling prion replication rate, infectivity, cross-species barrier and PrP(Sc) glycoform ratio.
25211026	0	15	theme	PrPSc	111:115	arg1	glycoform					117:125	PrPSc glycoform	111:125	PrPSc glycoform	111:125	Sialylation of prion protein controls the rate of prion amplification, the cross-species barrier, the ratio of PrPSc glycoform and prion infectivity.
25211026	3	16	theme	host	525:528	arg1	C					534:534	C	534:534	C	534:534	Among the key parameters that control prion replication identified over the years are amino acid sequence of host PrP(C) and the strain-specific structure of PrPSc.
25211026	3	16	theme	host	525:528	arg1	PrP					530:532	host PrP	525:532	host PrP(C)	525:535	Among the key parameters that control prion replication identified over the years are amino acid sequence of host PrP(C) and the strain-specific structure of PrPSc.
25211026	1	17	theme	conformational	203:216	arg1	change					218:223	conformational change	203:223	conformational change of the cellular form of the prion protein (PrP(C))	203:274	The central event underlying prion diseases involves conformational change of the cellular form of the prion protein (PrP(C)) into the disease-associated, transmissible form (PrP(Sc)).
25211026	4	18	theme	unappreciated	624:636	arg1	role					638:641	the previously unappreciated role	609:641	the previously unappreciated role of sialylation of PrP(C) glycans in prion pathogenesis, including its role in controlling prion replication rate, infectivity, cross-species barrier and PrP(Sc) glycoform ratio	609:818	The current work highlights the previously unappreciated role of sialylation of PrP(C) glycans in prion pathogenesis, including its role in controlling prion replication rate, infectivity, cross-species barrier and PrP(Sc) glycoform ratio.
25211026	5	19	theme	Protein	922:928	arg1	Misfolding					930:939	Protein Misfolding Cyclic Amplification (PMCAb)	922:968	Protein Misfolding Cyclic Amplification (PMCAb)	922:968	The current study demonstrates that undersialylated PrP(C) is selected during prion amplification in Protein Misfolding Cyclic Amplification (PMCAb) at the expense of oversialylated PrP(C).
25211026	11	20	gly	sialylation	1645:1655	arg1	PrP					1667:1669	PrP	1667:1669	PrP(C)	1667:1672	Surprisingly, the sialylation status of PrP(C) was also found to control PrP(Sc) glycoform ratio.
25211026	11	20	gly	sialylation	1645:1655	arg1	C					1671:1671	C	1671:1671	C	1671:1671	Surprisingly, the sialylation status of PrP(C) was also found to control PrP(Sc) glycoform ratio.
25211026	6	21	link	PMCAb-derived	1024:1036	arg1	Sc					1042:1043	Sc	1042:1043	Sc	1042:1043	As a result, PMCAb-derived PrP(Sc) was less sialylated than brain-derived PrP(Sc).
25211026	6	21	link	PMCAb-derived	1024:1036	arg1	result					1016:1021	a result	1014:1021	a result	1014:1021	As a result, PMCAb-derived PrP(Sc) was less sialylated than brain-derived PrP(Sc).
25211026	6	21	link	PMCAb-derived	1024:1036	arg1	PrP					1038:1040	PMCAb-derived PrP	1024:1040	PMCAb-derived PrP(Sc)	1024:1044	As a result, PMCAb-derived PrP(Sc) was less sialylated than brain-derived PrP(Sc).
25211026	0	22	theme	infectivity	137:147	arg1	ratio					102:106	the ratio	98:106	the ratio of PrPSc glycoform and prion infectivity	98:147	Sialylation of prion protein controls the rate of prion amplification, the cross-species barrier, the ratio of PrPSc glycoform and prion infectivity.
25211026	0	22	theme	infectivity	137:147	arg1	amplification					56:68	prion amplification	50:68	prion amplification	50:68	Sialylation of prion protein controls the rate of prion amplification, the cross-species barrier, the ratio of PrPSc glycoform and prion infectivity.
25211026	5	23	theme	Cyclic	941:946	arg1	PMCAb					963:967	PMCAb	963:967	PMCAb	963:967	The current study demonstrates that undersialylated PrP(C) is selected during prion amplification in Protein Misfolding Cyclic Amplification (PMCAb) at the expense of oversialylated PrP(C).
25211026	5	23	theme	Cyclic	941:946	arg1	Amplification					948:960	Cyclic Amplification	941:960	Protein Misfolding Cyclic Amplification (PMCAb)	922:968	The current study demonstrates that undersialylated PrP(C) is selected during prion amplification in Protein Misfolding Cyclic Amplification (PMCAb) at the expense of oversialylated PrP(C).
25211026	0	24	gly	glycoform	117:125	arg1	PrPSc					111:115	PrPSc glycoform	111:125	PrPSc glycoform	111:125	Sialylation of prion protein controls the rate of prion amplification, the cross-species barrier, the ratio of PrPSc glycoform and prion infectivity.
25211026	5	25	gly	undersialylated	857:871	arg1	C					877:877	C	877:877	C	877:877	The current study demonstrates that undersialylated PrP(C) is selected during prion amplification in Protein Misfolding Cyclic Amplification (PMCAb) at the expense of oversialylated PrP(C).
25211026	5	25	gly	undersialylated	857:871	arg1	PrP					873:875	undersialylated PrP	857:875	undersialylated PrP(C)	857:878	The current study demonstrates that undersialylated PrP(C) is selected during prion amplification in Protein Misfolding Cyclic Amplification (PMCAb) at the expense of oversialylated PrP(C).
25211026	7	26	theme	PMCAb-derived	1167:1179	arg1	material					1181:1188	PMCAb-derived material	1167:1188	PMCAb-derived material	1167:1188	A decrease in PrPSc sialylation correlated with a drop in infectivity of PMCAb-derived material.
25211026	12	27	theme	higher	1779:1784	arg1	percentage					1786:1795	a higher percentage	1777:1795	a higher percentage of the diglycosylated glycoform in PrP(Sc)	1777:1838	A decrease in Pr(PC) sialylation levels resulted in a higher percentage of the diglycosylated glycoform in PrP(Sc).
25211026	0	28	theme	prion	50:54	arg1	barrier					89:95	the cross-species barrier	71:95	the cross-species barrier	71:95	Sialylation of prion protein controls the rate of prion amplification, the cross-species barrier, the ratio of PrPSc glycoform and prion infectivity.
25211026	0	28	theme	prion	50:54	arg1	ratio					102:106	the ratio	98:106	the ratio of PrPSc glycoform and prion infectivity	98:147	Sialylation of prion protein controls the rate of prion amplification, the cross-species barrier, the ratio of PrPSc glycoform and prion infectivity.
25211026	0	28	theme	prion	50:54	arg1	amplification					56:68	prion amplification	50:68	prion amplification	50:68	Sialylation of prion protein controls the rate of prion amplification, the cross-species barrier, the ratio of PrPSc glycoform and prion infectivity.
25211026	5	29	theme	PrP	1003:1005	arg1	expense					977:983	the expense	973:983	the expense of oversialylated PrP(C)	973:1008	The current study demonstrates that undersialylated PrP(C) is selected during prion amplification in Protein Misfolding Cyclic Amplification (PMCAb) at the expense of oversialylated PrP(C).
25211026	10	30	theme	prion	1608:1612	arg1	replication					1614:1624	homologous and heterologous prion replication	1580:1624	homologous and heterologous prion replication	1580:1624	These results suggest that the negative charge of sialic acid controls the energy barrier of homologous and heterologous prion replication.
25211026	4	31	from	pathogenesis	685:696	arg1	role					638:641	the previously unappreciated role	609:641	the previously unappreciated role of sialylation of PrP(C) glycans in prion pathogenesis, including its role in controlling prion replication rate, infectivity, cross-species barrier and PrP(Sc) glycoform ratio	609:818	The current work highlights the previously unappreciated role of sialylation of PrP(C) glycans in prion pathogenesis, including its role in controlling prion replication rate, infectivity, cross-species barrier and PrP(Sc) glycoform ratio.
25211026	13	32	theme	spleen-derived	1959:1972	arg1	PrP					1974:1976	spleen-derived PrP	1959:1976	spleen-derived PrP(C)	1959:1979	2D analysis of charge distribution revealed that the sialylation status of brain-derived PrP(C) differed from that of spleen-derived PrP(C).
25211026	13	32	theme	spleen-derived	1959:1972	arg1	C					1978:1978	C	1978:1978	C	1978:1978	2D analysis of charge distribution revealed that the sialylation status of brain-derived PrP(C) differed from that of spleen-derived PrP(C).
25211026	14	33	theme	sialidase	2005:2013	arg1	Neu1					2015:2018	lysosomal sialidase Neu1	1995:2018	lysosomal sialidase Neu1	1995:2018	Knocking out lysosomal sialidase Neu1 did not change the sialylation status of brain-derived PrP(C), suggesting that Neu1 is not responsible for desialylation of PrP(C).
25211026	12	34	gly	glycoform	1819:1827	arg2	PrP					1832:1834	PrP	1832:1834	PrP(Sc)	1832:1838	A decrease in Pr(PC) sialylation levels resulted in a higher percentage of the diglycosylated glycoform in PrP(Sc).
25211026	12	34	gly	glycoform	1819:1827	arg2	Sc					1836:1837	Sc	1836:1837	Sc	1836:1837	A decrease in Pr(PC) sialylation levels resulted in a higher percentage of the diglycosylated glycoform in PrP(Sc).
25211026	5	35	from	expense	977:983	arg1	amplification					905:917	prion amplification	899:917	prion amplification in Protein Misfolding Cyclic Amplification (PMCAb) at the expense of oversialylated PrP(C)	899:1008	The current study demonstrates that undersialylated PrP(C) is selected during prion amplification in Protein Misfolding Cyclic Amplification (PMCAb) at the expense of oversialylated PrP(C).
25211026	0	36	theme	cross-species	75:87	arg1	barrier					89:95	the cross-species barrier	71:95	the cross-species barrier	71:95	Sialylation of prion protein controls the rate of prion amplification, the cross-species barrier, the ratio of PrPSc glycoform and prion infectivity.
25211026	0	36	theme	cross-species	75:87	arg1	amplification					56:68	prion amplification	50:68	prion amplification	50:68	Sialylation of prion protein controls the rate of prion amplification, the cross-species barrier, the ratio of PrPSc glycoform and prion infectivity.
25211026	15	37	theme	unappreciated	2191:2203	arg1	role					2205:2208	previously unappreciated role	2180:2208	previously unappreciated role of PrP(C) sialylation in prion diseases	2180:2248	The current work highlights previously unappreciated role of PrP(C) sialylation in prion diseases and opens multiple new research directions, including development of new therapeutic approaches.
25211026	5	38	theme	undersialylated	857:871	arg1	C					877:877	C	877:877	C	877:877	The current study demonstrates that undersialylated PrP(C) is selected during prion amplification in Protein Misfolding Cyclic Amplification (PMCAb) at the expense of oversialylated PrP(C).
25211026	5	38	theme	undersialylated	857:871	arg1	PrP					873:875	undersialylated PrP	857:875	undersialylated PrP(C)	857:878	The current study demonstrates that undersialylated PrP(C) is selected during prion amplification in Protein Misfolding Cyclic Amplification (PMCAb) at the expense of oversialylated PrP(C).
25211026	15	39	theme	new	2319:2321	arg1	approaches					2335:2344	new therapeutic approaches	2319:2344	new therapeutic approaches	2319:2344	The current work highlights previously unappreciated role of PrP(C) sialylation in prion diseases and opens multiple new research directions, including development of new therapeutic approaches.
25211026	7	40	link	PMCAb-derived	1167:1179	arg1	material					1181:1188	PMCAb-derived material	1167:1188	PMCAb-derived material	1167:1188	A decrease in PrPSc sialylation correlated with a drop in infectivity of PMCAb-derived material.
25211026	0	41	theme	amplification	56:68	arg1	rate					42:45	the rate	38:45	the rate of prion amplification, the cross-species barrier, the ratio of PrPSc glycoform and prion infectivity	38:147	Sialylation of prion protein controls the rate of prion amplification, the cross-species barrier, the ratio of PrPSc glycoform and prion infectivity.
25211026	8	42	theme	enzymatic	1205:1213	arg1	de-sialylation					1215:1228	enzymatic de-sialylation	1205:1228	enzymatic de-sialylation of PrP(C) using sialidase	1205:1254	Nevertheless, enzymatic de-sialylation of PrP(C) using sialidase was found to increase the rate of PrP(Sc) amplification in PMCAb from 10- to 10,000-fold in a strain-dependent manner.
25211026	14	43	theme	PrP	2144:2146	arg1	desialylation					2127:2139	desialylation	2127:2139	desialylation of PrP(C)	2127:2149	Knocking out lysosomal sialidase Neu1 did not change the sialylation status of brain-derived PrP(C), suggesting that Neu1 is not responsible for desialylation of PrP(C).
25211026	4	44	dep	rate	751:754	arg1	ratio					814:818	glycoform ratio	804:818	glycoform ratio	804:818	The current work highlights the previously unappreciated role of sialylation of PrP(C) glycans in prion pathogenesis, including its role in controlling prion replication rate, infectivity, cross-species barrier and PrP(Sc) glycoform ratio.
25211026	1	45	theme	protein	259:265	arg1	form					241:244	the cellular form	228:244	the cellular form of the prion protein (PrP(C))	228:274	The central event underlying prion diseases involves conformational change of the cellular form of the prion protein (PrP(C)) into the disease-associated, transmissible form (PrP(Sc)).
25211026	15	46	theme	current	2156:2162	arg1	work					2164:2167	The current work	2152:2167	The current work	2152:2167	The current work highlights previously unappreciated role of PrP(C) sialylation in prion diseases and opens multiple new research directions, including development of new therapeutic approaches.
25211026	5	47	theme	current	825:831	arg1	study					833:837	The current study	821:837	The current study	821:837	The current study demonstrates that undersialylated PrP(C) is selected during prion amplification in Protein Misfolding Cyclic Amplification (PMCAb) at the expense of oversialylated PrP(C).
25211026	10	48	theme	replication	1614:1624	arg1	barrier					1569:1575	the energy barrier	1558:1575	the energy barrier of homologous and heterologous prion replication	1558:1624	These results suggest that the negative charge of sialic acid controls the energy barrier of homologous and heterologous prion replication.
25211026	11	49	theme	PrP	1700:1702	arg1	ratio					1718:1722	PrP(Sc) glycoform ratio	1700:1722	PrP(Sc) glycoform ratio	1700:1722	Surprisingly, the sialylation status of PrP(C) was also found to control PrP(Sc) glycoform ratio.
25211026	0	50	theme	prion	15:19	arg1	protein					21:27	prion protein	15:27	prion protein	15:27	Sialylation of prion protein controls the rate of prion amplification, the cross-species barrier, the ratio of PrPSc glycoform and prion infectivity.
25211026	2	51	theme	N-glycosylation	393:407	arg1	sites					409:413	two conserved N-glycosylation sites	379:413	two conserved N-glycosylation sites	379:413	Pr(PC) is a sialoglycoprotein that contains two conserved N-glycosylation sites.
25211026	8	52	theme	strain-dependent	1350:1365	arg1	manner					1367:1372	a strain-dependent manner	1348:1372	a strain-dependent manner	1348:1372	Nevertheless, enzymatic de-sialylation of PrP(C) using sialidase was found to increase the rate of PrP(Sc) amplification in PMCAb from 10- to 10,000-fold in a strain-dependent manner.
25211026	13	53	gly	sialylation	1894:1904	arg1	C					1934:1934	C	1934:1934	C	1934:1934	2D analysis of charge distribution revealed that the sialylation status of brain-derived PrP(C) differed from that of spleen-derived PrP(C).
25211026	13	53	gly	sialylation	1894:1904	arg1	PrP					1930:1932	brain-derived PrP	1916:1932	brain-derived PrP(C)	1916:1935	2D analysis of charge distribution revealed that the sialylation status of brain-derived PrP(C) differed from that of spleen-derived PrP(C).
25211026	13	54	theme	brain-derived	1916:1928	arg1	C					1934:1934	C	1934:1934	C	1934:1934	2D analysis of charge distribution revealed that the sialylation status of brain-derived PrP(C) differed from that of spleen-derived PrP(C).
25211026	13	54	theme	brain-derived	1916:1928	arg1	PrP					1930:1932	brain-derived PrP	1916:1932	brain-derived PrP(C)	1916:1935	2D analysis of charge distribution revealed that the sialylation status of brain-derived PrP(C) differed from that of spleen-derived PrP(C).
25211026	14	55	gly	sialylation	2039:2049	arg1	C					2079:2079	C	2079:2079	C	2079:2079	Knocking out lysosomal sialidase Neu1 did not change the sialylation status of brain-derived PrP(C), suggesting that Neu1 is not responsible for desialylation of PrP(C).
25211026	14	55	gly	sialylation	2039:2049	arg1	PrP					2075:2077	brain-derived PrP	2061:2077	brain-derived PrP(C)	2061:2080	Knocking out lysosomal sialidase Neu1 did not change the sialylation status of brain-derived PrP(C), suggesting that Neu1 is not responsible for desialylation of PrP(C).
25211026	5	56	theme	prion	899:903	arg1	amplification					905:917	prion amplification	899:917	prion amplification in Protein Misfolding Cyclic Amplification (PMCAb) at the expense of oversialylated PrP(C)	899:1008	The current study demonstrates that undersialylated PrP(C) is selected during prion amplification in Protein Misfolding Cyclic Amplification (PMCAb) at the expense of oversialylated PrP(C).
25211026	11	57	theme	sialylation	1645:1655	arg1	status					1657:1662	the sialylation status	1641:1662	the sialylation status of PrP(C)	1641:1672	Surprisingly, the sialylation status of PrP(C) was also found to control PrP(Sc) glycoform ratio.
25211026	8	58	gly	de-sialylation	1215:1228	arg1	C					1237:1237	C	1237:1237	C	1237:1237	Nevertheless, enzymatic de-sialylation of PrP(C) using sialidase was found to increase the rate of PrP(Sc) amplification in PMCAb from 10- to 10,000-fold in a strain-dependent manner.
25211026	8	58	gly	de-sialylation	1215:1228	arg1	PrP					1233:1235	PrP	1233:1235	PrP(C) using sialidase	1233:1254	Nevertheless, enzymatic de-sialylation of PrP(C) using sialidase was found to increase the rate of PrP(Sc) amplification in PMCAb from 10- to 10,000-fold in a strain-dependent manner.
25211026	9	59	theme	prion	1457:1461	arg1	amplification					1463:1475	prion amplification	1457:1475	prion amplification in PMCAb	1457:1484	Moreover, de-sialylation of PrP(C) reduced or eliminated a species barrier of for prion amplification in PMCAb.
25211026	7	60	theme	PrPSc	1108:1112	arg1	sialylation					1114:1124	PrPSc sialylation	1108:1124	PrPSc sialylation	1108:1124	A decrease in PrPSc sialylation correlated with a drop in infectivity of PMCAb-derived material.
25211026	14	61	theme	brain-derived	2061:2073	arg1	C					2079:2079	C	2079:2079	C	2079:2079	Knocking out lysosomal sialidase Neu1 did not change the sialylation status of brain-derived PrP(C), suggesting that Neu1 is not responsible for desialylation of PrP(C).
25211026	14	61	theme	brain-derived	2061:2073	arg1	PrP					2075:2077	brain-derived PrP	2061:2077	brain-derived PrP(C)	2061:2080	Knocking out lysosomal sialidase Neu1 did not change the sialylation status of brain-derived PrP(C), suggesting that Neu1 is not responsible for desialylation of PrP(C).
25211026	9	62	theme	PrP	1403:1405	arg1	de-sialylation					1385:1398	de-sialylation	1385:1398	de-sialylation of PrP(C)	1385:1408	Moreover, de-sialylation of PrP(C) reduced or eliminated a species barrier of for prion amplification in PMCAb.
25211026	4	63	from	role	638:641	arg1	pathogenesis					685:696	prion pathogenesis	679:696	prion pathogenesis	679:696	The current work highlights the previously unappreciated role of sialylation of PrP(C) glycans in prion pathogenesis, including its role in controlling prion replication rate, infectivity, cross-species barrier and PrP(Sc) glycoform ratio.
25211026	7	64	from	drop	1144:1147	arg1	infectivity					1152:1162	infectivity	1152:1162	infectivity of PMCAb-derived material	1152:1188	A decrease in PrPSc sialylation correlated with a drop in infectivity of PMCAb-derived material.
25211026	15	65	from	role	2205:2208	arg1	diseases					2241:2248	prion diseases	2235:2248	prion diseases	2235:2248	The current work highlights previously unappreciated role of PrP(C) sialylation in prion diseases and opens multiple new research directions, including development of new therapeutic approaches.
25211026	3	66	theme	PrPSc	574:578	arg1	structure					561:569	the strain-specific structure	541:569	the strain-specific structure of PrPSc	541:578	Among the key parameters that control prion replication identified over the years are amino acid sequence of host PrP(C) and the strain-specific structure of PrPSc.
25211026	3	66	theme	PrPSc	574:578	arg1	sequence					513:520	amino acid sequence	502:520	amino acid sequence of host PrP(C)	502:535	Among the key parameters that control prion replication identified over the years are amino acid sequence of host PrP(C) and the strain-specific structure of PrPSc.
25211026	10	67	theme	negative	1518:1525	arg1	charge					1527:1532	the negative charge	1514:1532	the negative charge of sialic acid	1514:1547	These results suggest that the negative charge of sialic acid controls the energy barrier of homologous and heterologous prion replication.
25211026	15	68	theme	prion	2235:2239	arg1	diseases					2241:2248	prion diseases	2235:2248	prion diseases	2235:2248	The current work highlights previously unappreciated role of PrP(C) sialylation in prion diseases and opens multiple new research directions, including development of new therapeutic approaches.
25211026	8	69	dep	10-	1326:1328	arg1	to					1330:1331	to	1330:1331	to	1330:1331	Nevertheless, enzymatic de-sialylation of PrP(C) using sialidase was found to increase the rate of PrP(Sc) amplification in PMCAb from 10- to 10,000-fold in a strain-dependent manner.
25211026	12	70	theme	sialylation	1746:1756	arg1	levels					1758:1763	Pr(PC) sialylation levels	1739:1763	Pr(PC) sialylation levels	1739:1763	A decrease in Pr(PC) sialylation levels resulted in a higher percentage of the diglycosylated glycoform in PrP(Sc).
25211026	3	71	theme	key	426:428	arg1	parameters					430:439	the key parameters	422:439	the key parameters that control prion replication identified over the years	422:496	Among the key parameters that control prion replication identified over the years are amino acid sequence of host PrP(C) and the strain-specific structure of PrPSc.
25211026	15	72	theme	sialylation	2220:2230	arg1	role					2205:2208	previously unappreciated role	2180:2208	previously unappreciated role of PrP(C) sialylation in prion diseases	2180:2248	The current work highlights previously unappreciated role of PrP(C) sialylation in prion diseases and opens multiple new research directions, including development of new therapeutic approaches.
25211026	1	73	theme	form	241:244	arg1	change					218:223	conformational change	203:223	conformational change of the cellular form of the prion protein (PrP(C))	203:274	The central event underlying prion diseases involves conformational change of the cellular form of the prion protein (PrP(C)) into the disease-associated, transmissible form (PrP(Sc)).
25211026	12	74	gly	diglycosylated	1804:1817	arg1	glycoform					1819:1827	the diglycosylated glycoform	1800:1827	the diglycosylated glycoform in PrP(Sc)	1800:1838	A decrease in Pr(PC) sialylation levels resulted in a higher percentage of the diglycosylated glycoform in PrP(Sc).
25211026	9	75	theme	species	1434:1440	arg1	barrier					1442:1448	a species barrier	1432:1448	a species barrier of for prion amplification in PMCAb	1432:1484	Moreover, de-sialylation of PrP(C) reduced or eliminated a species barrier of for prion amplification in PMCAb.
25211026	2	76	gly	N-glycosylation	393:407	arg2	sites					409:413	two conserved N-glycosylation sites	379:413	two conserved N-glycosylation sites	379:413	Pr(PC) is a sialoglycoprotein that contains two conserved N-glycosylation sites.
25211026	2	76	gly	N-glycosylation	393:407	arg2	two					379:381	two	379:381	two	379:381	Pr(PC) is a sialoglycoprotein that contains two conserved N-glycosylation sites.
25211026	10	77	theme	acid	1544:1547	arg1	charge					1527:1532	the negative charge	1514:1532	the negative charge of sialic acid	1514:1547	These results suggest that the negative charge of sialic acid controls the energy barrier of homologous and heterologous prion replication.
25211026	12	78	theme	Pr	1739:1740	arg1	levels					1758:1763	Pr(PC) sialylation levels	1739:1763	Pr(PC) sialylation levels	1739:1763	A decrease in Pr(PC) sialylation levels resulted in a higher percentage of the diglycosylated glycoform in PrP(Sc).
25211026	4	79	theme	cross-species	770:782	arg1	barrier					784:790	cross-species barrier	770:790	cross-species barrier	770:790	The current work highlights the previously unappreciated role of sialylation of PrP(C) glycans in prion pathogenesis, including its role in controlling prion replication rate, infectivity, cross-species barrier and PrP(Sc) glycoform ratio.
25211026	5	80	dep	Misfolding	930:939	arg1	PMCAb					963:967	PMCAb	963:967	PMCAb	963:967	The current study demonstrates that undersialylated PrP(C) is selected during prion amplification in Protein Misfolding Cyclic Amplification (PMCAb) at the expense of oversialylated PrP(C).
25211026	5	80	dep	Misfolding	930:939	arg1	Amplification					948:960	Cyclic Amplification	941:960	Protein Misfolding Cyclic Amplification (PMCAb)	922:968	The current study demonstrates that undersialylated PrP(C) is selected during prion amplification in Protein Misfolding Cyclic Amplification (PMCAb) at the expense of oversialylated PrP(C).
25211026	6	81	gly	sialylated	1055:1064	arg1	Sc					1042:1043	Sc	1042:1043	Sc	1042:1043	As a result, PMCAb-derived PrP(Sc) was less sialylated than brain-derived PrP(Sc).
25211026	6	81	gly	sialylated	1055:1064	arg1	result					1016:1021	a result	1014:1021	a result	1014:1021	As a result, PMCAb-derived PrP(Sc) was less sialylated than brain-derived PrP(Sc).
25211026	6	81	gly	sialylated	1055:1064	arg1	PrP					1038:1040	PMCAb-derived PrP	1024:1040	PMCAb-derived PrP(Sc)	1024:1044	As a result, PMCAb-derived PrP(Sc) was less sialylated than brain-derived PrP(Sc).
25211026	14	82	theme	PrP	2075:2077	arg1	status					2051:2056	the sialylation status	2035:2056	the sialylation status of brain-derived PrP(C)	2035:2080	Knocking out lysosomal sialidase Neu1 did not change the sialylation status of brain-derived PrP(C), suggesting that Neu1 is not responsible for desialylation of PrP(C).
25211026	3	83	theme	prion	454:458	arg1	replication					460:470	prion replication	454:470	prion replication	454:470	Among the key parameters that control prion replication identified over the years are amino acid sequence of host PrP(C) and the strain-specific structure of PrPSc.
25211026	4	84	theme	prion	733:737	arg1	rate					751:754	prion replication rate	733:754	prion replication rate	733:754	The current work highlights the previously unappreciated role of sialylation of PrP(C) glycans in prion pathogenesis, including its role in controlling prion replication rate, infectivity, cross-species barrier and PrP(Sc) glycoform ratio.
25211026	9	85	gly	de-sialylation	1385:1398	arg1	C					1407:1407	C	1407:1407	C	1407:1407	Moreover, de-sialylation of PrP(C) reduced or eliminated a species barrier of for prion amplification in PMCAb.
25211026	9	85	gly	de-sialylation	1385:1398	arg1	PrP					1403:1405	PrP	1403:1405	PrP(C)	1403:1408	Moreover, de-sialylation of PrP(C) reduced or eliminated a species barrier of for prion amplification in PMCAb.
25211026	9	86	from	amplification	1463:1475	arg1	PMCAb					1480:1484	PMCAb	1480:1484	PMCAb	1480:1484	Moreover, de-sialylation of PrP(C) reduced or eliminated a species barrier of for prion amplification in PMCAb.
25211026	14	87	theme	sialylation	2039:2049	arg1	status					2051:2056	the sialylation status	2035:2056	the sialylation status of brain-derived PrP(C)	2035:2080	Knocking out lysosomal sialidase Neu1 did not change the sialylation status of brain-derived PrP(C), suggesting that Neu1 is not responsible for desialylation of PrP(C).
25211026	15	88	theme	research	2273:2280	arg1	directions					2282:2291	multiple new research directions	2260:2291	multiple new research directions	2260:2291	The current work highlights previously unappreciated role of PrP(C) sialylation in prion diseases and opens multiple new research directions, including development of new therapeutic approaches.
25211026	15	88	theme	research	2273:2280	arg1	development					2304:2314	development	2304:2314	development of new therapeutic approaches	2304:2344	The current work highlights previously unappreciated role of PrP(C) sialylation in prion diseases and opens multiple new research directions, including development of new therapeutic approaches.
25211026	1	89	theme	prion	179:183	arg1	diseases					185:192	prion diseases	179:192	prion diseases	179:192	The central event underlying prion diseases involves conformational change of the cellular form of the prion protein (PrP(C)) into the disease-associated, transmissible form (PrP(Sc)).
25211026	10	90	theme	energy	1562:1567	arg1	barrier					1569:1575	the energy barrier	1558:1575	the energy barrier of homologous and heterologous prion replication	1558:1624	These results suggest that the negative charge of sialic acid controls the energy barrier of homologous and heterologous prion replication.
25211026	14	91	gly	desialylation	2127:2139	arg1	PrP					2144:2146	PrP	2144:2146	PrP(C)	2144:2149	Knocking out lysosomal sialidase Neu1 did not change the sialylation status of brain-derived PrP(C), suggesting that Neu1 is not responsible for desialylation of PrP(C).
25211026	14	91	gly	desialylation	2127:2139	arg1	C					2148:2148	C	2148:2148	C	2148:2148	Knocking out lysosomal sialidase Neu1 did not change the sialylation status of brain-derived PrP(C), suggesting that Neu1 is not responsible for desialylation of PrP(C).
25211026	15	92	gly	sialylation	2220:2230	arg1	prion					2235:2239	prion diseases	2235:2248	prion diseases	2235:2248	The current work highlights previously unappreciated role of PrP(C) sialylation in prion diseases and opens multiple new research directions, including development of new therapeutic approaches.
25211026	5	93	gly	oversialylated	988:1001	arg1	C					1007:1007	C	1007:1007	C	1007:1007	The current study demonstrates that undersialylated PrP(C) is selected during prion amplification in Protein Misfolding Cyclic Amplification (PMCAb) at the expense of oversialylated PrP(C).
25211026	5	93	gly	oversialylated	988:1001	arg1	PrP					1003:1005	oversialylated PrP	988:1005	oversialylated PrP(C)	988:1008	The current study demonstrates that undersialylated PrP(C) is selected during prion amplification in Protein Misfolding Cyclic Amplification (PMCAb) at the expense of oversialylated PrP(C).
25211026	15	94	theme	new	2269:2271	arg1	directions					2282:2291	multiple new research directions	2260:2291	multiple new research directions	2260:2291	The current work highlights previously unappreciated role of PrP(C) sialylation in prion diseases and opens multiple new research directions, including development of new therapeutic approaches.
25211026	15	94	theme	new	2269:2271	arg1	development					2304:2314	development	2304:2314	development of new therapeutic approaches	2304:2344	The current work highlights previously unappreciated role of PrP(C) sialylation in prion diseases and opens multiple new research directions, including development of new therapeutic approaches.
25211026	6	95	link	brain-derived	1071:1083	arg1	Sc					1089:1090	Sc	1089:1090	Sc	1089:1090	As a result, PMCAb-derived PrP(Sc) was less sialylated than brain-derived PrP(Sc).
25211026	6	95	link	brain-derived	1071:1083	arg1	PrP					1085:1087	brain-derived PrP	1071:1087	brain-derived PrP(Sc)	1071:1091	As a result, PMCAb-derived PrP(Sc) was less sialylated than brain-derived PrP(Sc).
25211026	0	96	theme	glycoform	117:125	arg1	ratio					102:106	the ratio	98:106	the ratio of PrPSc glycoform and prion infectivity	98:147	Sialylation of prion protein controls the rate of prion amplification, the cross-species barrier, the ratio of PrPSc glycoform and prion infectivity.
25211026	0	96	theme	glycoform	117:125	arg1	amplification					56:68	prion amplification	50:68	prion amplification	50:68	Sialylation of prion protein controls the rate of prion amplification, the cross-species barrier, the ratio of PrPSc glycoform and prion infectivity.
25211026	13	97	theme	2D	1841:1842	arg1	analysis					1844:1851	2D analysis	1841:1851	2D analysis of charge distribution	1841:1874	2D analysis of charge distribution revealed that the sialylation status of brain-derived PrP(C) differed from that of spleen-derived PrP(C).
25211026	10	98	theme	heterologous	1595:1606	arg1	replication					1614:1624	homologous and heterologous prion replication	1580:1624	homologous and heterologous prion replication	1580:1624	These results suggest that the negative charge of sialic acid controls the energy barrier of homologous and heterologous prion replication.
25211026	15	99	theme	multiple	2260:2267	arg1	directions					2282:2291	multiple new research directions	2260:2291	multiple new research directions	2260:2291	The current work highlights previously unappreciated role of PrP(C) sialylation in prion diseases and opens multiple new research directions, including development of new therapeutic approaches.
25211026	15	99	theme	multiple	2260:2267	arg1	development					2304:2314	development	2304:2314	development of new therapeutic approaches	2304:2344	The current work highlights previously unappreciated role of PrP(C) sialylation in prion diseases and opens multiple new research directions, including development of new therapeutic approaches.
25211026	6	100	theme	brain-derived	1071:1083	arg1	Sc					1089:1090	Sc	1089:1090	Sc	1089:1090	As a result, PMCAb-derived PrP(Sc) was less sialylated than brain-derived PrP(Sc).
25211026	6	100	theme	brain-derived	1071:1083	arg1	PrP					1085:1087	brain-derived PrP	1071:1087	brain-derived PrP(Sc)	1071:1091	As a result, PMCAb-derived PrP(Sc) was less sialylated than brain-derived PrP(Sc).
25211026	0	101	theme	prion	131:135	arg1	infectivity					137:147	prion infectivity	131:147	prion infectivity	131:147	Sialylation of prion protein controls the rate of prion amplification, the cross-species barrier, the ratio of PrPSc glycoform and prion infectivity.
25211026	3	102	theme	acid	508:511	arg1	sequence					513:520	amino acid sequence	502:520	amino acid sequence of host PrP(C)	502:535	Among the key parameters that control prion replication identified over the years are amino acid sequence of host PrP(C) and the strain-specific structure of PrPSc.
25211026	12	103	from	glycoform	1819:1827	arg1	Sc					1836:1837	Sc	1836:1837	Sc	1836:1837	A decrease in Pr(PC) sialylation levels resulted in a higher percentage of the diglycosylated glycoform in PrP(Sc).
25211026	12	103	from	glycoform	1819:1827	arg1	PrP					1832:1834	PrP	1832:1834	PrP(Sc)	1832:1838	A decrease in Pr(PC) sialylation levels resulted in a higher percentage of the diglycosylated glycoform in PrP(Sc).
25211026	8	104	theme	PrP	1290:1292	arg1	amplification					1298:1310	PrP(Sc) amplification	1290:1310	PrP(Sc) amplification	1290:1310	Nevertheless, enzymatic de-sialylation of PrP(C) using sialidase was found to increase the rate of PrP(Sc) amplification in PMCAb from 10- to 10,000-fold in a strain-dependent manner.
25211026	15	105	theme	PrP	2213:2215	arg1	sialylation					2220:2230	PrP(C) sialylation	2213:2230	PrP(C) sialylation	2213:2230	The current work highlights previously unappreciated role of PrP(C) sialylation in prion diseases and opens multiple new research directions, including development of new therapeutic approaches.
25211026	12	106	theme	glycoform	1819:1827	arg1	percentage					1786:1795	a higher percentage	1777:1795	a higher percentage of the diglycosylated glycoform in PrP(Sc)	1777:1838	A decrease in Pr(PC) sialylation levels resulted in a higher percentage of the diglycosylated glycoform in PrP(Sc).
25211026	8	107	from	rate	1282:1285	arg1	PMCAb					1315:1319	PMCAb	1315:1319	PMCAb	1315:1319	Nevertheless, enzymatic de-sialylation of PrP(C) using sialidase was found to increase the rate of PrP(Sc) amplification in PMCAb from 10- to 10,000-fold in a strain-dependent manner.
25211026	3	108	theme	PrP	530:532	arg1	structure					561:569	the strain-specific structure	541:569	the strain-specific structure of PrPSc	541:578	Among the key parameters that control prion replication identified over the years are amino acid sequence of host PrP(C) and the strain-specific structure of PrPSc.
25211026	3	108	theme	PrP	530:532	arg1	sequence					513:520	amino acid sequence	502:520	amino acid sequence of host PrP(C)	502:535	Among the key parameters that control prion replication identified over the years are amino acid sequence of host PrP(C) and the strain-specific structure of PrPSc.
25211026	12	109	from	percentage	1786:1795	arg1	Sc					1836:1837	Sc	1836:1837	Sc	1836:1837	A decrease in Pr(PC) sialylation levels resulted in a higher percentage of the diglycosylated glycoform in PrP(Sc).
25211026	12	109	from	percentage	1786:1795	arg1	PrP					1832:1834	PrP	1832:1834	PrP(Sc)	1832:1838	A decrease in Pr(PC) sialylation levels resulted in a higher percentage of the diglycosylated glycoform in PrP(Sc).
25211026	8	110	theme	amplification	1298:1310	arg1	rate					1282:1285	the rate	1278:1285	the rate of PrP(Sc) amplification in PMCAb	1278:1319	Nevertheless, enzymatic de-sialylation of PrP(C) using sialidase was found to increase the rate of PrP(Sc) amplification in PMCAb from 10- to 10,000-fold in a strain-dependent manner.
25211026	7	111	theme	material	1181:1188	arg1	infectivity					1152:1162	infectivity	1152:1162	infectivity of PMCAb-derived material	1152:1188	A decrease in PrPSc sialylation correlated with a drop in infectivity of PMCAb-derived material.
25211026	4	112	theme	current	585:591	arg1	work					593:596	The current work	581:596	The current work	581:596	The current work highlights the previously unappreciated role of sialylation of PrP(C) glycans in prion pathogenesis, including its role in controlling prion replication rate, infectivity, cross-species barrier and PrP(Sc) glycoform ratio.
25211026	15	113	theme	approaches	2335:2344	arg1	development					2304:2314	development	2304:2314	development of new therapeutic approaches	2304:2344	The current work highlights previously unappreciated role of PrP(C) sialylation in prion diseases and opens multiple new research directions, including development of new therapeutic approaches.
25211026	1	114	theme	disease-associated	285:302	arg1	form					319:322	the disease-associated, transmissible form	281:322	form	319:322	The central event underlying prion diseases involves conformational change of the cellular form of the prion protein (PrP(C)) into the disease-associated, transmissible form (PrP(Sc)).
25211026	1	115	dep	protein	259:265	arg1	PrP					268:270	PrP	268:270	PrP(C)	268:273	The central event underlying prion diseases involves conformational change of the cellular form of the prion protein (PrP(C)) into the disease-associated, transmissible form (PrP(Sc)).
25211026	1	115	dep	protein	259:265	arg1	C					272:272	C	272:272	C	272:272	The central event underlying prion diseases involves conformational change of the cellular form of the prion protein (PrP(C)) into the disease-associated, transmissible form (PrP(Sc)).
25211026	14	116	theme	lysosomal	1995:2003	arg1	Neu1					2015:2018	lysosomal sialidase Neu1	1995:2018	lysosomal sialidase Neu1	1995:2018	Knocking out lysosomal sialidase Neu1 did not change the sialylation status of brain-derived PrP(C), suggesting that Neu1 is not responsible for desialylation of PrP(C).
25211026	15	117	theme	therapeutic	2323:2333	arg1	approaches					2335:2344	new therapeutic approaches	2319:2344	new therapeutic approaches	2319:2344	The current work highlights previously unappreciated role of PrP(C) sialylation in prion diseases and opens multiple new research directions, including development of new therapeutic approaches.
25211026	1	118	theme	transmissible	305:317	arg1	form					319:322	the disease-associated, transmissible form	281:322	form	319:322	The central event underlying prion diseases involves conformational change of the cellular form of the prion protein (PrP(C)) into the disease-associated, transmissible form (PrP(Sc)).
25211026	4	119	from	glycans	668:674	arg1	pathogenesis					685:696	prion pathogenesis	679:696	prion pathogenesis	679:696	The current work highlights the previously unappreciated role of sialylation of PrP(C) glycans in prion pathogenesis, including its role in controlling prion replication rate, infectivity, cross-species barrier and PrP(Sc) glycoform ratio.
25211026	4	120	theme	glycans	668:674	arg1	role					638:641	the previously unappreciated role	609:641	the previously unappreciated role of sialylation of PrP(C) glycans in prion pathogenesis, including its role in controlling prion replication rate, infectivity, cross-species barrier and PrP(Sc) glycoform ratio	609:818	The current work highlights the previously unappreciated role of sialylation of PrP(C) glycans in prion pathogenesis, including its role in controlling prion replication rate, infectivity, cross-species barrier and PrP(Sc) glycoform ratio.
25211026	5	121	theme	oversialylated	988:1001	arg1	C					1007:1007	C	1007:1007	C	1007:1007	The current study demonstrates that undersialylated PrP(C) is selected during prion amplification in Protein Misfolding Cyclic Amplification (PMCAb) at the expense of oversialylated PrP(C).
25211026	5	121	theme	oversialylated	988:1001	arg1	PrP					1003:1005	oversialylated PrP	988:1005	oversialylated PrP(C)	988:1008	The current study demonstrates that undersialylated PrP(C) is selected during prion amplification in Protein Misfolding Cyclic Amplification (PMCAb) at the expense of oversialylated PrP(C).
25211026	14	122	link	brain-derived	2061:2073	arg1	C					2079:2079	C	2079:2079	C	2079:2079	Knocking out lysosomal sialidase Neu1 did not change the sialylation status of brain-derived PrP(C), suggesting that Neu1 is not responsible for desialylation of PrP(C).
25211026	14	122	link	brain-derived	2061:2073	arg1	PrP					2075:2077	brain-derived PrP	2061:2077	brain-derived PrP(C)	2061:2080	Knocking out lysosomal sialidase Neu1 did not change the sialylation status of brain-derived PrP(C), suggesting that Neu1 is not responsible for desialylation of PrP(C).
25211026	4	123	theme	sialylation	646:656	arg1	role					638:641	the previously unappreciated role	609:641	the previously unappreciated role of sialylation of PrP(C) glycans in prion pathogenesis, including its role in controlling prion replication rate, infectivity, cross-species barrier and PrP(Sc) glycoform ratio	609:818	The current work highlights the previously unappreciated role of sialylation of PrP(C) glycans in prion pathogenesis, including its role in controlling prion replication rate, infectivity, cross-species barrier and PrP(Sc) glycoform ratio.
25211026	0	124	theme	protein	21:27	arg1	Sialylation					0:10	Sialylation	0:10	Sialylation of prion protein	0:27	Sialylation of prion protein controls the rate of prion amplification, the cross-species barrier, the ratio of PrPSc glycoform and prion infectivity.
25211026	12	125	from	PrP	1832:1834	arg1	percentage					1786:1795	a higher percentage	1777:1795	a higher percentage of the diglycosylated glycoform in PrP(Sc)	1777:1838	A decrease in Pr(PC) sialylation levels resulted in a higher percentage of the diglycosylated glycoform in PrP(Sc).
25211026	11	126	theme	glycoform	1708:1716	arg1	ratio					1718:1722	PrP(Sc) glycoform ratio	1700:1722	PrP(Sc) glycoform ratio	1700:1722	Surprisingly, the sialylation status of PrP(C) was also found to control PrP(Sc) glycoform ratio.
25211026	3	127	theme	strain-specific	545:559	arg1	structure					561:569	the strain-specific structure	541:569	the strain-specific structure of PrPSc	541:578	Among the key parameters that control prion replication identified over the years are amino acid sequence of host PrP(C) and the strain-specific structure of PrPSc.
25211026	4	128	theme	PrP	661:663	arg1	role					713:716	its role	709:716	its role in controlling prion replication rate, infectivity, cross-species barrier and PrP(Sc) glycoform ratio	709:818	The current work highlights the previously unappreciated role of sialylation of PrP(C) glycans in prion pathogenesis, including its role in controlling prion replication rate, infectivity, cross-species barrier and PrP(Sc) glycoform ratio.
25211026	4	128	theme	PrP	661:663	arg1	glycans					668:674	PrP(C) glycans	661:674	PrP(C) glycans	661:674	The current work highlights the previously unappreciated role of sialylation of PrP(C) glycans in prion pathogenesis, including its role in controlling prion replication rate, infectivity, cross-species barrier and PrP(Sc) glycoform ratio.
25211026	12	129	from	decrease	1727:1734	arg1	levels					1758:1763	Pr(PC) sialylation levels	1739:1763	Pr(PC) sialylation levels	1739:1763	A decrease in Pr(PC) sialylation levels resulted in a higher percentage of the diglycosylated glycoform in PrP(Sc).
25211026	2	130	theme	conserved	383:391	arg1	sites					409:413	two conserved N-glycosylation sites	379:413	two conserved N-glycosylation sites	379:413	Pr(PC) is a sialoglycoprotein that contains two conserved N-glycosylation sites.
25211026	13	131	link	spleen-derived	1959:1972	arg1	PrP					1974:1976	spleen-derived PrP	1959:1976	spleen-derived PrP(C)	1959:1979	2D analysis of charge distribution revealed that the sialylation status of brain-derived PrP(C) differed from that of spleen-derived PrP(C).
25211026	13	131	link	spleen-derived	1959:1972	arg1	C					1978:1978	C	1978:1978	C	1978:1978	2D analysis of charge distribution revealed that the sialylation status of brain-derived PrP(C) differed from that of spleen-derived PrP(C).
25211026	8	132	theme	PrP	1233:1235	arg1	de-sialylation					1215:1228	enzymatic de-sialylation	1205:1228	enzymatic de-sialylation of PrP(C) using sialidase	1205:1254	Nevertheless, enzymatic de-sialylation of PrP(C) using sialidase was found to increase the rate of PrP(Sc) amplification in PMCAb from 10- to 10,000-fold in a strain-dependent manner.
25211026	13	133	theme	PrP	1930:1932	arg1	status					1906:1911	the sialylation status	1890:1911	the sialylation status of brain-derived PrP(C)	1890:1935	2D analysis of charge distribution revealed that the sialylation status of brain-derived PrP(C) differed from that of spleen-derived PrP(C).
25211026	1	134	dep	form	319:322	arg1	Sc					329:330	Sc	329:330	Sc	329:330	The central event underlying prion diseases involves conformational change of the cellular form of the prion protein (PrP(C)) into the disease-associated, transmissible form (PrP(Sc)).
25211026	1	134	dep	form	319:322	arg1	PrP					325:327	PrP	325:327	PrP(Sc)	325:331	The central event underlying prion diseases involves conformational change of the cellular form of the prion protein (PrP(C)) into the disease-associated, transmissible form (PrP(Sc)).
25211026	13	135	link	brain-derived	1916:1928	arg1	C					1934:1934	C	1934:1934	C	1934:1934	2D analysis of charge distribution revealed that the sialylation status of brain-derived PrP(C) differed from that of spleen-derived PrP(C).
25211026	13	135	link	brain-derived	1916:1928	arg1	PrP					1930:1932	brain-derived PrP	1916:1932	brain-derived PrP(C)	1916:1935	2D analysis of charge distribution revealed that the sialylation status of brain-derived PrP(C) differed from that of spleen-derived PrP(C).
25211026	2	136	gly	sialoglycoprotein	347:363	arg1	sialoglycoprotein					347:363	a sialoglycoprotein	345:363	a sialoglycoprotein that contains two conserved N-glycosylation sites	345:413	Pr(PC) is a sialoglycoprotein that contains two conserved N-glycosylation sites.
25211026	2	136	gly	sialoglycoprotein	347:363	arg1	Pr					335:336	Pr	335:336	Pr(PC)	335:340	Pr(PC) is a sialoglycoprotein that contains two conserved N-glycosylation sites.
25211026	4	137	theme	prion	679:683	arg1	pathogenesis					685:696	prion pathogenesis	679:696	prion pathogenesis	679:696	The current work highlights the previously unappreciated role of sialylation of PrP(C) glycans in prion pathogenesis, including its role in controlling prion replication rate, infectivity, cross-species barrier and PrP(Sc) glycoform ratio.
25211026	13	138	theme	sialylation	1894:1904	arg1	status					1906:1911	the sialylation status	1890:1911	the sialylation status of brain-derived PrP(C)	1890:1935	2D analysis of charge distribution revealed that the sialylation status of brain-derived PrP(C) differed from that of spleen-derived PrP(C).
25211026	1	139	theme	cellular	232:239	arg1	form					241:244	the cellular form	228:244	the cellular form of the prion protein (PrP(C))	228:274	The central event underlying prion diseases involves conformational change of the cellular form of the prion protein (PrP(C)) into the disease-associated, transmissible form (PrP(Sc)).
26063800	7	0	theme	genes	1517:1521	arg1	regulation					1497:1506	the regulation	1493:1506	the regulation of these genes that have roles in human diseases, such as IgA nephropathy and cancer	1493:1591	These results are important in understanding the regulation of these genes that have roles in human diseases, such as IgA nephropathy and cancer.
26063800	4	1	contain	contain	856:862	arg1	cCpG-II					832:838	cCpG-II	832:838	cCpG-II	832:838	Using luciferase reporter assays, site-directed mutagenesis, ChIP assays, and mithramycin A treatment, we identified the core promoters within cCpG-II and tCpG, which contain two binding sites for Krüppel-like transcription factors, including SP1/SP3, respectively.
26063800	4	1	contain	contain	856:862	arg1	tCpG					844:847	tCpG	844:847	tCpG	844:847	Using luciferase reporter assays, site-directed mutagenesis, ChIP assays, and mithramycin A treatment, we identified the core promoters within cCpG-II and tCpG, which contain two binding sites for Krüppel-like transcription factors, including SP1/SP3, respectively.
26063800	4	1	contain	contain	856:862	arg2	sites					876:880	two binding sites	864:880	two binding sites for Krüppel-like transcription factors, including SP1/SP3	864:938	Using luciferase reporter assays, site-directed mutagenesis, ChIP assays, and mithramycin A treatment, we identified the core promoters within cCpG-II and tCpG, which contain two binding sites for Krüppel-like transcription factors, including SP1/SP3, respectively.
26063800	1	2	theme	O-glycans	235:243	arg1	biosynthesis					208:219	the biosynthesis	204:219	the biosynthesis of mucin type O-glycans on glycoproteins	204:260	The T-synthase (core 1 β3-galactosyltransferase) and its molecular chaperone Cosmc regulate the biosynthesis of mucin type O-glycans on glycoproteins, and evidence suggests that both T-synthase and Cosmc are transcriptionally suppressed in several human diseases, although the transcriptional regulation of these two genes is not understood.
26063800	5	3	theme	Cosmc	1059:1063	arg1	promoter					1070:1077	the Cosmc core promoter	1055:1077	the Cosmc core promoter	1055:1077	Methylome analysis of Tn4 B cells, which harbor a silenced Cosmc, confirmed the hypermethylation of the Cosmc core promoter but not for T-synthase.
26063800	2	4	theme	T-synthase	521:530	arg1	transcription					532:544	T-synthase transcription	521:544	T-synthase transcription	521:544	Here, we characterized the promoters essential for human Cosmc and T-synthase transcription.
26063800	4	5	theme	mithramycin	767:777	arg1	A					779:779	mithramycin A	767:779	mithramycin A treatment	767:789	Using luciferase reporter assays, site-directed mutagenesis, ChIP assays, and mithramycin A treatment, we identified the core promoters within cCpG-II and tCpG, which contain two binding sites for Krüppel-like transcription factors, including SP1/SP3, respectively.
26063800	6	6	theme	members	1275:1281	arg1	family					1265:1270	the specificity protein/Krüppel-like transcription factor family	1207:1270	the specificity protein/Krüppel-like transcription factor family of members, which explains their ubiquitous and coordinated expression	1207:1341	These results demonstrate that Cosmc and T-synthase are transcriptionally regulated at a basal level by the specificity protein/Krüppel-like transcription factor family of members, which explains their ubiquitous and coordinated expression, and also indicate that they are differentially epigenetically regulated beyond X chromosome imprinting.
26063800	6	7	theme	basal	1192:1196	arg1	level					1198:1202	a basal level	1190:1202	a basal level	1190:1202	These results demonstrate that Cosmc and T-synthase are transcriptionally regulated at a basal level by the specificity protein/Krüppel-like transcription factor family of members, which explains their ubiquitous and coordinated expression, and also indicate that they are differentially epigenetically regulated beyond X chromosome imprinting.
26063800	5	8	theme	B	981:981	arg1	cells					983:987	Tn4 B cells	977:987	Tn4 B cells	977:987	Methylome analysis of Tn4 B cells, which harbor a silenced Cosmc, confirmed the hypermethylation of the Cosmc core promoter but not for T-synthase.
26063800	1	9	theme	several	352:358	arg1	diseases					366:373	several human diseases	352:373	several human diseases	352:373	The T-synthase (core 1 β3-galactosyltransferase) and its molecular chaperone Cosmc regulate the biosynthesis of mucin type O-glycans on glycoproteins, and evidence suggests that both T-synthase and Cosmc are transcriptionally suppressed in several human diseases, although the transcriptional regulation of these two genes is not understood.
26063800	4	10	theme	ChIP	750:753	arg1	assays					755:760	ChIP assays	750:760	ChIP assays	750:760	Using luciferase reporter assays, site-directed mutagenesis, ChIP assays, and mithramycin A treatment, we identified the core promoters within cCpG-II and tCpG, which contain two binding sites for Krüppel-like transcription factors, including SP1/SP3, respectively.
26063800	3	11	theme	upstream	551:558	arg1	regions					560:566	The upstream regions	547:566	The upstream regions of the genes	547:579	The upstream regions of the genes lack a conventional TATA box but contain CpG islands, cCpG-I and cCpG-II for Cosmc and tCpG for T-synthase.
26063800	7	12	theme	IgA	1566:1568	arg1	nephropathy					1570:1580	IgA nephropathy	1566:1580	IgA nephropathy	1566:1580	These results are important in understanding the regulation of these genes that have roles in human diseases, such as IgA nephropathy and cancer.
26063800	0	13	theme	Epigenetic	89:98	arg1	Regulation					100:109	Epigenetic Regulation	89:109	Epigenetic Regulation	89:109	Promoters of Human Cosmc and T-synthase Genes Are Similar in Structure, Yet Different in Epigenetic Regulation.
26063800	1	14	theme	human	360:364	arg1	diseases					366:373	several human diseases	352:373	several human diseases	352:373	The T-synthase (core 1 β3-galactosyltransferase) and its molecular chaperone Cosmc regulate the biosynthesis of mucin type O-glycans on glycoproteins, and evidence suggests that both T-synthase and Cosmc are transcriptionally suppressed in several human diseases, although the transcriptional regulation of these two genes is not understood.
26063800	6	15	theme	ubiquitous	1305:1314	arg1	expression					1332:1341	their ubiquitous and coordinated expression	1299:1341	their ubiquitous and coordinated expression	1299:1341	These results demonstrate that Cosmc and T-synthase are transcriptionally regulated at a basal level by the specificity protein/Krüppel-like transcription factor family of members, which explains their ubiquitous and coordinated expression, and also indicate that they are differentially epigenetically regulated beyond X chromosome imprinting.
26063800	7	16	contain	have	1528:1531	arg2	roles					1533:1537	roles	1533:1537	roles	1533:1537	These results are important in understanding the regulation of these genes that have roles in human diseases, such as IgA nephropathy and cancer.
26063800	7	16	contain	have	1528:1531	arg1	genes					1517:1521	these genes	1511:1521	these genes that have roles in human diseases, such as IgA nephropathy and cancer	1511:1591	These results are important in understanding the regulation of these genes that have roles in human diseases, such as IgA nephropathy and cancer.
26063800	2	17	theme	human	505:509	arg1	Cosmc					511:515	human Cosmc	505:515	human Cosmc	505:515	Here, we characterized the promoters essential for human Cosmc and T-synthase transcription.
26063800	5	18	theme	core	1065:1068	arg1	promoter					1070:1077	the Cosmc core promoter	1055:1077	the Cosmc core promoter	1055:1077	Methylome analysis of Tn4 B cells, which harbor a silenced Cosmc, confirmed the hypermethylation of the Cosmc core promoter but not for T-synthase.
26063800	6	19	theme	transcription	1244:1256	arg1	factor					1258:1263	specificity protein/Krüppel-like transcription factor	1211:1263	the specificity protein/Krüppel-like transcription factor family of members, which explains their ubiquitous and coordinated expression	1207:1341	These results demonstrate that Cosmc and T-synthase are transcriptionally regulated at a basal level by the specificity protein/Krüppel-like transcription factor family of members, which explains their ubiquitous and coordinated expression, and also indicate that they are differentially epigenetically regulated beyond X chromosome imprinting.
26063800	4	20	theme	site-directed	723:735	arg1	mutagenesis					737:747	site-directed mutagenesis	723:747	site-directed mutagenesis	723:747	Using luciferase reporter assays, site-directed mutagenesis, ChIP assays, and mithramycin A treatment, we identified the core promoters within cCpG-II and tCpG, which contain two binding sites for Krüppel-like transcription factors, including SP1/SP3, respectively.
26063800	5	21	theme	promoter	1070:1077	arg1	hypermethylation					1035:1050	the hypermethylation	1031:1050	the hypermethylation of the Cosmc core promoter but not for T-synthase	1031:1100	Methylome analysis of Tn4 B cells, which harbor a silenced Cosmc, confirmed the hypermethylation of the Cosmc core promoter but not for T-synthase.
26063800	6	22	theme	protein/Krüppel-like	1223:1242	arg1	factor					1258:1263	specificity protein/Krüppel-like transcription factor	1211:1263	the specificity protein/Krüppel-like transcription factor family of members, which explains their ubiquitous and coordinated expression	1207:1341	These results demonstrate that Cosmc and T-synthase are transcriptionally regulated at a basal level by the specificity protein/Krüppel-like transcription factor family of members, which explains their ubiquitous and coordinated expression, and also indicate that they are differentially epigenetically regulated beyond X chromosome imprinting.
26063800	3	23	theme	genes	575:579	arg1	regions					560:566	The upstream regions	547:566	The upstream regions of the genes	547:579	The upstream regions of the genes lack a conventional TATA box but contain CpG islands, cCpG-I and cCpG-II for Cosmc and tCpG for T-synthase.
26063800	1	24	theme	molecular	169:177	arg1	Cosmc					189:193	its molecular chaperone Cosmc	165:193	its molecular chaperone Cosmc	165:193	The T-synthase (core 1 β3-galactosyltransferase) and its molecular chaperone Cosmc regulate the biosynthesis of mucin type O-glycans on glycoproteins, and evidence suggests that both T-synthase and Cosmc are transcriptionally suppressed in several human diseases, although the transcriptional regulation of these two genes is not understood.
26063800	0	25	theme	Cosmc	19:23	arg1	Genes					40:44	Human Cosmc and T-synthase Genes	13:44	Human Cosmc and T-synthase Genes	13:44	Promoters of Human Cosmc and T-synthase Genes Are Similar in Structure, Yet Different in Epigenetic Regulation.
26063800	1	26	from	glycoproteins	248:260	arg1	biosynthesis					208:219	the biosynthesis	204:219	the biosynthesis of mucin type O-glycans on glycoproteins	204:260	The T-synthase (core 1 β3-galactosyltransferase) and its molecular chaperone Cosmc regulate the biosynthesis of mucin type O-glycans on glycoproteins, and evidence suggests that both T-synthase and Cosmc are transcriptionally suppressed in several human diseases, although the transcriptional regulation of these two genes is not understood.
26063800	1	27	gly	glycoproteins	248:260	arg1	glycoproteins					248:260	glycoproteins	248:260	glycoproteins	248:260	The T-synthase (core 1 β3-galactosyltransferase) and its molecular chaperone Cosmc regulate the biosynthesis of mucin type O-glycans on glycoproteins, and evidence suggests that both T-synthase and Cosmc are transcriptionally suppressed in several human diseases, although the transcriptional regulation of these two genes is not understood.
26063800	6	28	theme	specificity	1211:1221	arg1	factor					1258:1263	specificity protein/Krüppel-like transcription factor	1211:1263	the specificity protein/Krüppel-like transcription factor family of members, which explains their ubiquitous and coordinated expression	1207:1341	These results demonstrate that Cosmc and T-synthase are transcriptionally regulated at a basal level by the specificity protein/Krüppel-like transcription factor family of members, which explains their ubiquitous and coordinated expression, and also indicate that they are differentially epigenetically regulated beyond X chromosome imprinting.
26063800	1	29	theme	chaperone	179:187	arg1	Cosmc					189:193	its molecular chaperone Cosmc	165:193	its molecular chaperone Cosmc	165:193	The T-synthase (core 1 β3-galactosyltransferase) and its molecular chaperone Cosmc regulate the biosynthesis of mucin type O-glycans on glycoproteins, and evidence suggests that both T-synthase and Cosmc are transcriptionally suppressed in several human diseases, although the transcriptional regulation of these two genes is not understood.
26063800	0	30	theme	Human	13:17	arg1	Genes					40:44	Human Cosmc and T-synthase Genes	13:44	Human Cosmc and T-synthase Genes	13:44	Promoters of Human Cosmc and T-synthase Genes Are Similar in Structure, Yet Different in Epigenetic Regulation.
26063800	4	31	theme	Krüppel-like	886:897	arg1	factors					913:919	Krüppel-like transcription factors	886:919	Krüppel-like transcription factors	886:919	Using luciferase reporter assays, site-directed mutagenesis, ChIP assays, and mithramycin A treatment, we identified the core promoters within cCpG-II and tCpG, which contain two binding sites for Krüppel-like transcription factors, including SP1/SP3, respectively.
26063800	4	31	theme	Krüppel-like	886:897	arg1	SP1/SP3					932:938	SP1/SP3	932:938	SP1/SP3	932:938	Using luciferase reporter assays, site-directed mutagenesis, ChIP assays, and mithramycin A treatment, we identified the core promoters within cCpG-II and tCpG, which contain two binding sites for Krüppel-like transcription factors, including SP1/SP3, respectively.
26063800	5	32	theme	Methylome	955:963	arg1	analysis					965:972	Methylome analysis	955:972	Methylome analysis	955:972	Methylome analysis of Tn4 B cells, which harbor a silenced Cosmc, confirmed the hypermethylation of the Cosmc core promoter but not for T-synthase.
26063800	0	33	theme	T-synthase	29:38	arg1	Genes					40:44	Human Cosmc and T-synthase Genes	13:44	Human Cosmc and T-synthase Genes	13:44	Promoters of Human Cosmc and T-synthase Genes Are Similar in Structure, Yet Different in Epigenetic Regulation.
26063800	1	34	from	O-glycans	235:243	arg1	glycoproteins					248:260	glycoproteins	248:260	glycoproteins	248:260	The T-synthase (core 1 β3-galactosyltransferase) and its molecular chaperone Cosmc regulate the biosynthesis of mucin type O-glycans on glycoproteins, and evidence suggests that both T-synthase and Cosmc are transcriptionally suppressed in several human diseases, although the transcriptional regulation of these two genes is not understood.
26063800	0	35	from	Structure	61:69	arg1	Similar					50:56	Similar	50:56	Similar	50:56	Promoters of Human Cosmc and T-synthase Genes Are Similar in Structure, Yet Different in Epigenetic Regulation.
26063800	3	36	theme	conventional	588:599	arg1	box					606:608	a conventional TATA box	586:608	a conventional TATA box	586:608	The upstream regions of the genes lack a conventional TATA box but contain CpG islands, cCpG-I and cCpG-II for Cosmc and tCpG for T-synthase.
26063800	6	37	theme	factor	1258:1263	arg1	family					1265:1270	the specificity protein/Krüppel-like transcription factor family	1207:1270	the specificity protein/Krüppel-like transcription factor family of members, which explains their ubiquitous and coordinated expression	1207:1341	These results demonstrate that Cosmc and T-synthase are transcriptionally regulated at a basal level by the specificity protein/Krüppel-like transcription factor family of members, which explains their ubiquitous and coordinated expression, and also indicate that they are differentially epigenetically regulated beyond X chromosome imprinting.
26063800	6	38	theme	chromosome	1425:1434	arg1	imprinting					1436:1445	X chromosome imprinting	1423:1445	X chromosome imprinting	1423:1445	These results demonstrate that Cosmc and T-synthase are transcriptionally regulated at a basal level by the specificity protein/Krüppel-like transcription factor family of members, which explains their ubiquitous and coordinated expression, and also indicate that they are differentially epigenetically regulated beyond X chromosome imprinting.
26063800	1	39	theme	transcriptional	389:403	arg1	regulation					405:414	the transcriptional regulation	385:414	the transcriptional regulation of these two genes	385:433	The T-synthase (core 1 β3-galactosyltransferase) and its molecular chaperone Cosmc regulate the biosynthesis of mucin type O-glycans on glycoproteins, and evidence suggests that both T-synthase and Cosmc are transcriptionally suppressed in several human diseases, although the transcriptional regulation of these two genes is not understood.
26063800	3	40	theme	TATA	601:604	arg1	box					606:608	a conventional TATA box	586:608	a conventional TATA box	586:608	The upstream regions of the genes lack a conventional TATA box but contain CpG islands, cCpG-I and cCpG-II for Cosmc and tCpG for T-synthase.
26063800	6	41	theme	X	1423:1423	arg1	chromosome					1425:1434	X chromosome	1423:1434	X chromosome imprinting	1423:1445	These results demonstrate that Cosmc and T-synthase are transcriptionally regulated at a basal level by the specificity protein/Krüppel-like transcription factor family of members, which explains their ubiquitous and coordinated expression, and also indicate that they are differentially epigenetically regulated beyond X chromosome imprinting.
26063800	4	42	theme	binding	868:874	arg1	sites					876:880	two binding sites	864:880	two binding sites for Krüppel-like transcription factors, including SP1/SP3	864:938	Using luciferase reporter assays, site-directed mutagenesis, ChIP assays, and mithramycin A treatment, we identified the core promoters within cCpG-II and tCpG, which contain two binding sites for Krüppel-like transcription factors, including SP1/SP3, respectively.
26063800	0	43	from	Similar	50:56	arg1	Structure					61:69	Structure	61:69	Structure	61:69	Promoters of Human Cosmc and T-synthase Genes Are Similar in Structure, Yet Different in Epigenetic Regulation.
26063800	0	43	from	Similar	50:56	arg1	Regulation					100:109	Epigenetic Regulation	89:109	Epigenetic Regulation	89:109	Promoters of Human Cosmc and T-synthase Genes Are Similar in Structure, Yet Different in Epigenetic Regulation.
26063800	4	44	theme	core	810:813	arg1	promoters					815:823	the core promoters	806:823	the core promoters	806:823	Using luciferase reporter assays, site-directed mutagenesis, ChIP assays, and mithramycin A treatment, we identified the core promoters within cCpG-II and tCpG, which contain two binding sites for Krüppel-like transcription factors, including SP1/SP3, respectively.
26063800	4	45	theme	luciferase	695:704	arg1	assays					715:720	luciferase reporter assays	695:720	luciferase reporter assays	695:720	Using luciferase reporter assays, site-directed mutagenesis, ChIP assays, and mithramycin A treatment, we identified the core promoters within cCpG-II and tCpG, which contain two binding sites for Krüppel-like transcription factors, including SP1/SP3, respectively.
26063800	0	46	theme	Genes	40:44	arg1	Promoters					0:8	Promoters	0:8	Promoters of Human Cosmc and T-synthase Genes	0:44	Promoters of Human Cosmc and T-synthase Genes Are Similar in Structure, Yet Different in Epigenetic Regulation.
26063800	4	47	theme	transcription	899:911	arg1	factors					913:919	Krüppel-like transcription factors	886:919	Krüppel-like transcription factors	886:919	Using luciferase reporter assays, site-directed mutagenesis, ChIP assays, and mithramycin A treatment, we identified the core promoters within cCpG-II and tCpG, which contain two binding sites for Krüppel-like transcription factors, including SP1/SP3, respectively.
26063800	4	47	theme	transcription	899:911	arg1	SP1/SP3					932:938	SP1/SP3	932:938	SP1/SP3	932:938	Using luciferase reporter assays, site-directed mutagenesis, ChIP assays, and mithramycin A treatment, we identified the core promoters within cCpG-II and tCpG, which contain two binding sites for Krüppel-like transcription factors, including SP1/SP3, respectively.
26063800	5	48	theme	silenced	1005:1012	arg1	Cosmc					1014:1018	a silenced Cosmc	1003:1018	a silenced Cosmc	1003:1018	Methylome analysis of Tn4 B cells, which harbor a silenced Cosmc, confirmed the hypermethylation of the Cosmc core promoter but not for T-synthase.
26063800	3	49	contain	contain	614:620	arg2	islands					626:632	CpG islands	622:632	CpG islands	622:632	The upstream regions of the genes lack a conventional TATA box but contain CpG islands, cCpG-I and cCpG-II for Cosmc and tCpG for T-synthase.
26063800	3	49	contain	contain	614:620	arg2	cCpG-II					646:652	cCpG-II	646:652	cCpG-II	646:652	The upstream regions of the genes lack a conventional TATA box but contain CpG islands, cCpG-I and cCpG-II for Cosmc and tCpG for T-synthase.
26063800	3	49	contain	contain	614:620	arg1	regions					560:566	The upstream regions	547:566	The upstream regions of the genes	547:579	The upstream regions of the genes lack a conventional TATA box but contain CpG islands, cCpG-I and cCpG-II for Cosmc and tCpG for T-synthase.
26063800	3	49	contain	contain	614:620	arg2	cCpG-I					635:640	cCpG-I	635:640	cCpG-I	635:640	The upstream regions of the genes lack a conventional TATA box but contain CpG islands, cCpG-I and cCpG-II for Cosmc and tCpG for T-synthase.
26063800	7	50	theme	human	1542:1546	arg1	nephropathy					1570:1580	IgA nephropathy	1566:1580	IgA nephropathy	1566:1580	These results are important in understanding the regulation of these genes that have roles in human diseases, such as IgA nephropathy and cancer.
26063800	7	50	theme	human	1542:1546	arg1	diseases					1548:1555	human diseases	1542:1555	human diseases	1542:1555	These results are important in understanding the regulation of these genes that have roles in human diseases, such as IgA nephropathy and cancer.
26063800	7	50	theme	human	1542:1546	arg1	cancer					1586:1591	cancer	1586:1591	cancer	1586:1591	These results are important in understanding the regulation of these genes that have roles in human diseases, such as IgA nephropathy and cancer.
26063800	1	51	from	biosynthesis	208:219	arg1	glycoproteins					248:260	glycoproteins	248:260	glycoproteins	248:260	The T-synthase (core 1 β3-galactosyltransferase) and its molecular chaperone Cosmc regulate the biosynthesis of mucin type O-glycans on glycoproteins, and evidence suggests that both T-synthase and Cosmc are transcriptionally suppressed in several human diseases, although the transcriptional regulation of these two genes is not understood.
26063800	5	52	theme	Tn4	977:979	arg1	cells					983:987	Tn4 B cells	977:987	Tn4 B cells	977:987	Methylome analysis of Tn4 B cells, which harbor a silenced Cosmc, confirmed the hypermethylation of the Cosmc core promoter but not for T-synthase.
26063800	4	53	theme	A	779:779	arg1	treatment					781:789	mithramycin A treatment	767:789	mithramycin A treatment	767:789	Using luciferase reporter assays, site-directed mutagenesis, ChIP assays, and mithramycin A treatment, we identified the core promoters within cCpG-II and tCpG, which contain two binding sites for Krüppel-like transcription factors, including SP1/SP3, respectively.
26063800	6	54	theme	coordinated	1320:1330	arg1	expression					1332:1341	their ubiquitous and coordinated expression	1299:1341	their ubiquitous and coordinated expression	1299:1341	These results demonstrate that Cosmc and T-synthase are transcriptionally regulated at a basal level by the specificity protein/Krüppel-like transcription factor family of members, which explains their ubiquitous and coordinated expression, and also indicate that they are differentially epigenetically regulated beyond X chromosome imprinting.
26063800	3	55	theme	CpG	622:624	arg1	cCpG-I					635:640	cCpG-I	635:640	cCpG-I	635:640	The upstream regions of the genes lack a conventional TATA box but contain CpG islands, cCpG-I and cCpG-II for Cosmc and tCpG for T-synthase.
26063800	3	55	theme	CpG	622:624	arg1	islands					626:632	CpG islands	622:632	CpG islands	622:632	The upstream regions of the genes lack a conventional TATA box but contain CpG islands, cCpG-I and cCpG-II for Cosmc and tCpG for T-synthase.
26063800	3	55	theme	CpG	622:624	arg1	cCpG-II					646:652	cCpG-II	646:652	cCpG-II	646:652	The upstream regions of the genes lack a conventional TATA box but contain CpG islands, cCpG-I and cCpG-II for Cosmc and tCpG for T-synthase.
26063800	4	56	theme	reporter	706:713	arg1	assays					715:720	luciferase reporter assays	695:720	luciferase reporter assays	695:720	Using luciferase reporter assays, site-directed mutagenesis, ChIP assays, and mithramycin A treatment, we identified the core promoters within cCpG-II and tCpG, which contain two binding sites for Krüppel-like transcription factors, including SP1/SP3, respectively.
26063800	1	57	theme	genes	429:433	arg1	regulation					405:414	the transcriptional regulation	385:414	the transcriptional regulation of these two genes	385:433	The T-synthase (core 1 β3-galactosyltransferase) and its molecular chaperone Cosmc regulate the biosynthesis of mucin type O-glycans on glycoproteins, and evidence suggests that both T-synthase and Cosmc are transcriptionally suppressed in several human diseases, although the transcriptional regulation of these two genes is not understood.
26063800	5	58	theme	cells	983:987	arg1	analysis					965:972	Methylome analysis	955:972	Methylome analysis	955:972	Methylome analysis of Tn4 B cells, which harbor a silenced Cosmc, confirmed the hypermethylation of the Cosmc core promoter but not for T-synthase.
26063800	1	59	theme	mucin	224:228	arg1	O-glycans					235:243	mucin type O-glycans	224:243	mucin type O-glycans on glycoproteins	224:260	The T-synthase (core 1 β3-galactosyltransferase) and its molecular chaperone Cosmc regulate the biosynthesis of mucin type O-glycans on glycoproteins, and evidence suggests that both T-synthase and Cosmc are transcriptionally suppressed in several human diseases, although the transcriptional regulation of these two genes is not understood.
26063800	2	60	theme	essential	491:499	arg1	promoters					481:489	the promoters	477:489	the promoters essential for human Cosmc and T-synthase transcription	477:544	Here, we characterized the promoters essential for human Cosmc and T-synthase transcription.
26063800	0	61	from	Regulation	100:109	arg1	Similar					50:56	Similar	50:56	Similar	50:56	Promoters of Human Cosmc and T-synthase Genes Are Similar in Structure, Yet Different in Epigenetic Regulation.
26063800	1	62	theme	core	128:131	arg1	T-synthase					116:125	The T-synthase	112:125	The T-synthase (core 1 β3-galactosyltransferase)	112:159	The T-synthase (core 1 β3-galactosyltransferase) and its molecular chaperone Cosmc regulate the biosynthesis of mucin type O-glycans on glycoproteins, and evidence suggests that both T-synthase and Cosmc are transcriptionally suppressed in several human diseases, although the transcriptional regulation of these two genes is not understood.
26063800	1	62	theme	core	128:131	arg1	β3-galactosyltransferase					135:158	core 1 β3-galactosyltransferase	128:158	core 1 β3-galactosyltransferase	128:158	The T-synthase (core 1 β3-galactosyltransferase) and its molecular chaperone Cosmc regulate the biosynthesis of mucin type O-glycans on glycoproteins, and evidence suggests that both T-synthase and Cosmc are transcriptionally suppressed in several human diseases, although the transcriptional regulation of these two genes is not understood.
26063800	1	63	theme	type	230:233	arg1	O-glycans					235:243	mucin type O-glycans	224:243	mucin type O-glycans on glycoproteins	224:260	The T-synthase (core 1 β3-galactosyltransferase) and its molecular chaperone Cosmc regulate the biosynthesis of mucin type O-glycans on glycoproteins, and evidence suggests that both T-synthase and Cosmc are transcriptionally suppressed in several human diseases, although the transcriptional regulation of these two genes is not understood.
26721884	13	0	theme	renal	1865:1869	arg1	cells					1871:1875	renal cells	1865:1875	renal cells	1865:1875	In summary, our results demonstrate the presence of an OS9-mediated ERAD pathway in renal cells that degrades immature NKCC2 proteins.
26721884	3	1	theme	study	524:528	arg1	aim					505:507	The aim	501:507	The aim of the present study	501:528	The aim of the present study was to identify the protein partners specifically involved in ERAD of NKCC2.
26721884	9	2	from	reduction	1260:1268	arg1	levels					1300:1305	the co-transporter protein levels	1273:1305	the co-transporter protein levels	1273:1305	Pulse-chase and cycloheximide-chase assays demonstrated that the marked reduction in the co-transporter protein levels was essentially due to increased protein degradation of the immature form of NKCC2.
26721884	9	3	theme	Pulse-chase	1188:1198	arg1	assays					1224:1229	Pulse-chase and cycloheximide-chase assays	1188:1229	Pulse-chase and cycloheximide-chase assays	1188:1229	Pulse-chase and cycloheximide-chase assays demonstrated that the marked reduction in the co-transporter protein levels was essentially due to increased protein degradation of the immature form of NKCC2.
26721884	1	4	theme	life-threatening	250:265	arg1	disease					274:280	a life-threatening kidney disease	248:280	a life-threatening kidney disease featuring arterial hypotension along with electrolyte abnormalities	248:348	Mutations in the renal specific Na-K-2Cl co-transporter (NKCC2) lead to type I Bartter syndrome, a life-threatening kidney disease featuring arterial hypotension along with electrolyte abnormalities.
26721884	1	4	theme	life-threatening	250:265	arg1	syndrome					238:245	type I Bartter syndrome	223:245	type I Bartter syndrome	223:245	Mutations in the renal specific Na-K-2Cl co-transporter (NKCC2) lead to type I Bartter syndrome, a life-threatening kidney disease featuring arterial hypotension along with electrolyte abnormalities.
26721884	8	5	theme	inhibitor	1171:1179	arg1	MG132					1181:1185	the proteasome inhibitor MG132	1156:1185	the proteasome inhibitor MG132	1156:1185	In cells overexpressing OS9, total cellular NKCC2 protein levels were markedly decreased, an effect blocked by the proteasome inhibitor MG132.
26721884	6	6	theme	OS9	875:877	arg1	association					879:889	OS9 association	875:889	OS9 association	875:889	Co-immunoprecipitation assays in renal cells revealed that OS9 association involves mainly the immature form of NKCC2.
26721884	13	7	theme	OS9-mediated	1836:1847	arg1	pathway					1854:1860	an OS9-mediated ERAD pathway	1833:1860	an OS9-mediated ERAD pathway	1833:1860	In summary, our results demonstrate the presence of an OS9-mediated ERAD pathway in renal cells that degrades immature NKCC2 proteins.
26721884	9	8	theme	cycloheximide-chase	1204:1222	arg1	assays					1224:1229	Pulse-chase and cycloheximide-chase assays	1188:1229	Pulse-chase and cycloheximide-chase assays	1188:1229	Pulse-chase and cycloheximide-chase assays demonstrated that the marked reduction in the co-transporter protein levels was essentially due to increased protein degradation of the immature form of NKCC2.
26721884	5	9	theme	specific	781:788	arg1	OS9					733:735	OS9	733:735	OS9 (amplified in osteosarcomas)	733:764	We identified OS9 (amplified in osteosarcomas) as a novel and specific binding partner of NKCC2.
26721884	5	9	theme	specific	781:788	arg1	partner					798:804	a novel and specific binding partner	769:804	a novel and specific binding partner of NKCC2	769:813	We identified OS9 (amplified in osteosarcomas) as a novel and specific binding partner of NKCC2.
26721884	1	10	theme	specific	174:181	arg1	NKCC2					208:212	NKCC2	208:212	NKCC2	208:212	Mutations in the renal specific Na-K-2Cl co-transporter (NKCC2) lead to type I Bartter syndrome, a life-threatening kidney disease featuring arterial hypotension along with electrolyte abnormalities.
26721884	1	10	theme	specific	174:181	arg1	co-transporter					192:205	the renal specific Na-K-2Cl co-transporter	164:205	the renal specific Na-K-2Cl co-transporter (NKCC2)	164:213	Mutations in the renal specific Na-K-2Cl co-transporter (NKCC2) lead to type I Bartter syndrome, a life-threatening kidney disease featuring arterial hypotension along with electrolyte abnormalities.
26721884	4	11	theme	yeast	664:668	arg1	assay					681:685	a yeast two-hybrid assay	662:685	a yeast two-hybrid assay using NKCC2 C terminus as bait	662:716	To this end, we screened a kidney cDNA library through a yeast two-hybrid assay using NKCC2 C terminus as bait.
26721884	6	12	from	assays	839:844	arg1	cells					855:859	renal cells	849:859	renal cells	849:859	Co-immunoprecipitation assays in renal cells revealed that OS9 association involves mainly the immature form of NKCC2.
26721884	10	13	theme	interfering	1429:1439	arg1	RNA					1441:1443	small interfering RNA	1423:1443	small interfering RNA	1423:1443	Conversely, knockdown of OS9 by small interfering RNA increased NKCC2 expression by increasing the co-transporter stability.
26721884	0	14	theme	Endoplasmic	97:107	arg1	Pathway					142:148	the Endoplasmic Reticulum-associated Degradation Pathway	93:148	the Endoplasmic Reticulum-associated Degradation Pathway	93:148	OS9 Protein Interacts with Na-K-2Cl Co-transporter (NKCC2) and Targets Its Immature Form for the Endoplasmic Reticulum-associated Degradation Pathway.
26721884	5	15	theme	NKCC2	809:813	arg1	OS9					733:735	OS9	733:735	OS9 (amplified in osteosarcomas)	733:764	We identified OS9 (amplified in osteosarcomas) as a novel and specific binding partner of NKCC2.
26721884	5	15	theme	NKCC2	809:813	arg1	partner					798:804	a novel and specific binding partner	769:804	a novel and specific binding partner of NKCC2	769:813	We identified OS9 (amplified in osteosarcomas) as a novel and specific binding partner of NKCC2.
26721884	4	16	theme	cDNA	641:644	arg1	library					646:652	a kidney cDNA library	632:652	a kidney cDNA library	632:652	To this end, we screened a kidney cDNA library through a yeast two-hybrid assay using NKCC2 C terminus as bait.
26721884	0	17	theme	Degradation	130:140	arg1	Pathway					142:148	the Endoplasmic Reticulum-associated Degradation Pathway	93:148	the Endoplasmic Reticulum-associated Degradation Pathway	93:148	OS9 Protein Interacts with Na-K-2Cl Co-transporter (NKCC2) and Targets Its Immature Form for the Endoplasmic Reticulum-associated Degradation Pathway.
26721884	13	18	from	presence	1821:1828	arg1	cells					1871:1875	renal cells	1865:1875	renal cells	1865:1875	In summary, our results demonstrate the presence of an OS9-mediated ERAD pathway in renal cells that degrades immature NKCC2 proteins.
26721884	6	19	theme	NKCC2	928:932	arg1	form					920:923	the immature form	907:923	the immature form of NKCC2	907:932	Co-immunoprecipitation assays in renal cells revealed that OS9 association involves mainly the immature form of NKCC2.
26721884	3	20	theme	NKCC2	600:604	arg1	ERAD					592:595	ERAD	592:595	ERAD of NKCC2	592:604	The aim of the present study was to identify the protein partners specifically involved in ERAD of NKCC2.
26721884	11	21	theme	mannose	1536:1542	arg1	receptor					1556:1563	mannose 6-phosphate receptor	1536:1563	the mannose 6-phosphate receptor homology domain of OS9	1532:1586	Inactivation of the mannose 6-phosphate receptor homology domain of OS9 had no effect on its action on NKCC2.
26721884	14	22	theme	specific	1979:1986	arg1	components					1968:1977	ERAD components	1963:1977	ERAD components specific to NKCC2 and its disease-causing mutants	1963:2027	The identification and selective modulation of ERAD components specific to NKCC2 and its disease-causing mutants might provide novel therapeutic strategies for the treatment of type I Bartter syndrome.
26721884	9	23	theme	protein	1292:1298	arg1	levels					1300:1305	the co-transporter protein levels	1273:1305	the co-transporter protein levels	1273:1305	Pulse-chase and cycloheximide-chase assays demonstrated that the marked reduction in the co-transporter protein levels was essentially due to increased protein degradation of the immature form of NKCC2.
26721884	11	24	from	action	1609:1614	arg1	NKCC2					1619:1623	NKCC2	1619:1623	NKCC2	1619:1623	Inactivation of the mannose 6-phosphate receptor homology domain of OS9 had no effect on its action on NKCC2.
26721884	4	25	theme	NKCC2	693:697	arg1	terminus					701:708	NKCC2 C terminus	693:708	NKCC2 C terminus	693:708	To this end, we screened a kidney cDNA library through a yeast two-hybrid assay using NKCC2 C terminus as bait.
26721884	11	26	theme	domain	1574:1579	arg1	Inactivation					1516:1527	Inactivation	1516:1527	Inactivation of the mannose 6-phosphate receptor homology domain of OS9	1516:1586	Inactivation of the mannose 6-phosphate receptor homology domain of OS9 had no effect on its action on NKCC2.
26721884	7	27	theme	immunocytochemistry	948:966	arg1	analysis					968:975	immunocytochemistry analysis	948:975	immunocytochemistry analysis	948:975	Accordingly, immunocytochemistry analysis showed that NKCC2 and OS9 co-localize at the endoplasmic reticulum.
26721884	12	28	theme	OS9-induced	1726:1736	arg1	degradation					1746:1756	OS9-induced protein degradation	1726:1756	OS9-induced protein degradation	1726:1756	In contrast, mutations of NKCC2 N-glycosylation sites abolished the effects of OS9, indicating that OS9-induced protein degradation is N-glycan-dependent.
26721884	11	29	theme	receptor	1556:1563	arg1	OS9					1584:1586	OS9	1584:1586	OS9	1584:1586	Inactivation of the mannose 6-phosphate receptor homology domain of OS9 had no effect on its action on NKCC2.
26721884	11	29	theme	receptor	1556:1563	arg1	domain					1574:1579	the mannose 6-phosphate receptor homology domain	1532:1579	the mannose 6-phosphate receptor homology domain of OS9	1532:1586	Inactivation of the mannose 6-phosphate receptor homology domain of OS9 had no effect on its action on NKCC2.
26721884	0	30	theme	Immature	75:82	arg1	Form					84:87	Its Immature Form	71:87	Its Immature Form	71:87	OS9 Protein Interacts with Na-K-2Cl Co-transporter (NKCC2) and Targets Its Immature Form for the Endoplasmic Reticulum-associated Degradation Pathway.
26721884	9	31	theme	increased	1330:1338	arg1	degradation					1348:1358	increased protein degradation	1330:1358	increased protein degradation of the immature form of NKCC2	1330:1388	Pulse-chase and cycloheximide-chase assays demonstrated that the marked reduction in the co-transporter protein levels was essentially due to increased protein degradation of the immature form of NKCC2.
26721884	11	32	contain	had	1588:1590	arg1	Inactivation					1516:1527	Inactivation	1516:1527	Inactivation of the mannose 6-phosphate receptor homology domain of OS9	1516:1586	Inactivation of the mannose 6-phosphate receptor homology domain of OS9 had no effect on its action on NKCC2.
26721884	11	32	contain	had	1588:1590	arg2	effect					1595:1600	no effect	1592:1600	no effect	1592:1600	Inactivation of the mannose 6-phosphate receptor homology domain of OS9 had no effect on its action on NKCC2.
26721884	12	33	theme	OS9	1705:1707	arg1	effects					1694:1700	the effects	1690:1700	the effects of OS9	1690:1707	In contrast, mutations of NKCC2 N-glycosylation sites abolished the effects of OS9, indicating that OS9-induced protein degradation is N-glycan-dependent.
26721884	10	34	theme	co-transporter	1490:1503	arg1	stability					1505:1513	the co-transporter stability	1486:1513	the co-transporter stability	1486:1513	Conversely, knockdown of OS9 by small interfering RNA increased NKCC2 expression by increasing the co-transporter stability.
26721884	0	35	theme	OS9	0:2	arg1	Protein					4:10	OS9 Protein	0:10	OS9 Protein	0:10	OS9 Protein Interacts with Na-K-2Cl Co-transporter (NKCC2) and Targets Its Immature Form for the Endoplasmic Reticulum-associated Degradation Pathway.
26721884	1	36	theme	kidney	267:272	arg1	disease					274:280	a life-threatening kidney disease	248:280	a life-threatening kidney disease featuring arterial hypotension along with electrolyte abnormalities	248:348	Mutations in the renal specific Na-K-2Cl co-transporter (NKCC2) lead to type I Bartter syndrome, a life-threatening kidney disease featuring arterial hypotension along with electrolyte abnormalities.
26721884	1	36	theme	kidney	267:272	arg1	syndrome					238:245	type I Bartter syndrome	223:245	type I Bartter syndrome	223:245	Mutations in the renal specific Na-K-2Cl co-transporter (NKCC2) lead to type I Bartter syndrome, a life-threatening kidney disease featuring arterial hypotension along with electrolyte abnormalities.
26721884	13	37	attach	presence	1821:1828	arg2	pathway					1854:1860	an OS9-mediated ERAD pathway	1833:1860	an OS9-mediated ERAD pathway	1833:1860	In summary, our results demonstrate the presence of an OS9-mediated ERAD pathway in renal cells that degrades immature NKCC2 proteins.
26721884	13	37	attach	presence	1821:1828	arg1	cells					1871:1875	renal cells	1865:1875	renal cells	1865:1875	In summary, our results demonstrate the presence of an OS9-mediated ERAD pathway in renal cells that degrades immature NKCC2 proteins.
26721884	12	38	theme	N-glycosylation	1658:1672	arg1	sites					1674:1678	NKCC2 N-glycosylation sites	1652:1678	NKCC2 N-glycosylation sites	1652:1678	In contrast, mutations of NKCC2 N-glycosylation sites abolished the effects of OS9, indicating that OS9-induced protein degradation is N-glycan-dependent.
26721884	2	39	theme	endoplasmic	448:458	arg1	ERAD					494:497	ERAD	494:497	ERAD	494:497	We have previously shown that NKCC2 and its disease-causing mutants are subject to regulation by endoplasmic reticulum-associated degradation (ERAD).
26721884	2	39	theme	endoplasmic	448:458	arg1	degradation					481:491	endoplasmic reticulum-associated degradation	448:491	endoplasmic reticulum-associated degradation (ERAD)	448:498	We have previously shown that NKCC2 and its disease-causing mutants are subject to regulation by endoplasmic reticulum-associated degradation (ERAD).
26721884	9	40	theme	form	1376:1379	arg1	degradation					1348:1358	increased protein degradation	1330:1358	increased protein degradation of the immature form of NKCC2	1330:1388	Pulse-chase and cycloheximide-chase assays demonstrated that the marked reduction in the co-transporter protein levels was essentially due to increased protein degradation of the immature form of NKCC2.
26721884	8	41	theme	total	1074:1078	arg1	levels					1103:1108	total cellular NKCC2 protein levels	1074:1108	total cellular NKCC2 protein levels	1074:1108	In cells overexpressing OS9, total cellular NKCC2 protein levels were markedly decreased, an effect blocked by the proteasome inhibitor MG132.
26721884	14	42	theme	novel	2043:2047	arg1	strategies					2061:2070	novel therapeutic strategies	2043:2070	novel therapeutic strategies	2043:2070	The identification and selective modulation of ERAD components specific to NKCC2 and its disease-causing mutants might provide novel therapeutic strategies for the treatment of type I Bartter syndrome.
26721884	13	43	theme	immature	1891:1898	arg1	proteins					1906:1913	immature NKCC2 proteins	1891:1913	immature NKCC2 proteins	1891:1913	In summary, our results demonstrate the presence of an OS9-mediated ERAD pathway in renal cells that degrades immature NKCC2 proteins.
26721884	0	44	theme	Na-K-2Cl	27:34	arg1	Co-transporter					36:49	Na-K-2Cl Co-transporter	27:49	Na-K-2Cl Co-transporter (NKCC2)	27:57	OS9 Protein Interacts with Na-K-2Cl Co-transporter (NKCC2) and Targets Its Immature Form for the Endoplasmic Reticulum-associated Degradation Pathway.
26721884	0	44	theme	Na-K-2Cl	27:34	arg1	NKCC2					52:56	NKCC2	52:56	NKCC2	52:56	OS9 Protein Interacts with Na-K-2Cl Co-transporter (NKCC2) and Targets Its Immature Form for the Endoplasmic Reticulum-associated Degradation Pathway.
26721884	9	45	theme	NKCC2	1384:1388	arg1	form					1376:1379	the immature form	1363:1379	the immature form of NKCC2	1363:1388	Pulse-chase and cycloheximide-chase assays demonstrated that the marked reduction in the co-transporter protein levels was essentially due to increased protein degradation of the immature form of NKCC2.
26721884	8	46	theme	NKCC2	1089:1093	arg1	levels					1103:1108	total cellular NKCC2 protein levels	1074:1108	total cellular NKCC2 protein levels	1074:1108	In cells overexpressing OS9, total cellular NKCC2 protein levels were markedly decreased, an effect blocked by the proteasome inhibitor MG132.
26721884	5	47	theme	novel	771:775	arg1	OS9					733:735	OS9	733:735	OS9 (amplified in osteosarcomas)	733:764	We identified OS9 (amplified in osteosarcomas) as a novel and specific binding partner of NKCC2.
26721884	5	47	theme	novel	771:775	arg1	partner					798:804	a novel and specific binding partner	769:804	a novel and specific binding partner of NKCC2	769:813	We identified OS9 (amplified in osteosarcomas) as a novel and specific binding partner of NKCC2.
26721884	6	48	theme	Co-immunoprecipitation	816:837	arg1	assays					839:844	Co-immunoprecipitation assays	816:844	Co-immunoprecipitation assays in renal cells	816:859	Co-immunoprecipitation assays in renal cells revealed that OS9 association involves mainly the immature form of NKCC2.
26721884	14	49	theme	disease-causing	2005:2019	arg1	mutants					2021:2027	its disease-causing mutants	2001:2027	its disease-causing mutants	2001:2027	The identification and selective modulation of ERAD components specific to NKCC2 and its disease-causing mutants might provide novel therapeutic strategies for the treatment of type I Bartter syndrome.
26721884	14	50	theme	Bartter	2100:2106	arg1	syndrome					2108:2115	Bartter syndrome	2100:2115	type I Bartter syndrome	2093:2115	The identification and selective modulation of ERAD components specific to NKCC2 and its disease-causing mutants might provide novel therapeutic strategies for the treatment of type I Bartter syndrome.
26721884	13	51	theme	ERAD	1849:1852	arg1	pathway					1854:1860	an OS9-mediated ERAD pathway	1833:1860	an OS9-mediated ERAD pathway	1833:1860	In summary, our results demonstrate the presence of an OS9-mediated ERAD pathway in renal cells that degrades immature NKCC2 proteins.
26721884	14	52	theme	components	1968:1977	arg1	identification					1920:1933	identification	1920:1933	identification	1920:1933	The identification and selective modulation of ERAD components specific to NKCC2 and its disease-causing mutants might provide novel therapeutic strategies for the treatment of type I Bartter syndrome.
26721884	14	52	theme	components	1968:1977	arg1	modulation					1949:1958	selective modulation	1939:1958	selective modulation	1939:1958	The identification and selective modulation of ERAD components specific to NKCC2 and its disease-causing mutants might provide novel therapeutic strategies for the treatment of type I Bartter syndrome.
26721884	6	53	theme	immature	911:918	arg1	form					920:923	the immature form	907:923	the immature form of NKCC2	907:932	Co-immunoprecipitation assays in renal cells revealed that OS9 association involves mainly the immature form of NKCC2.
26721884	3	54	theme	present	516:522	arg1	study					524:528	the present study	512:528	the present study	512:528	The aim of the present study was to identify the protein partners specifically involved in ERAD of NKCC2.
26721884	10	55	theme	OS9	1416:1418	arg1	knockdown					1403:1411	knockdown	1403:1411	knockdown of OS9 by small interfering RNA	1403:1443	Conversely, knockdown of OS9 by small interfering RNA increased NKCC2 expression by increasing the co-transporter stability.
26721884	3	56	theme	protein	550:556	arg1	partners					558:565	the protein partners	546:565	the protein partners specifically involved in ERAD of NKCC2	546:604	The aim of the present study was to identify the protein partners specifically involved in ERAD of NKCC2.
26721884	11	57	theme	OS9	1584:1586	arg1	OS9					1584:1586	OS9	1584:1586	OS9	1584:1586	Inactivation of the mannose 6-phosphate receptor homology domain of OS9 had no effect on its action on NKCC2.
26721884	11	57	theme	OS9	1584:1586	arg1	domain					1574:1579	the mannose 6-phosphate receptor homology domain	1532:1579	the mannose 6-phosphate receptor homology domain of OS9	1532:1586	Inactivation of the mannose 6-phosphate receptor homology domain of OS9 had no effect on its action on NKCC2.
26721884	14	58	theme	ERAD	1963:1966	arg1	components					1968:1977	ERAD components	1963:1977	ERAD components specific to NKCC2 and its disease-causing mutants	1963:2027	The identification and selective modulation of ERAD components specific to NKCC2 and its disease-causing mutants might provide novel therapeutic strategies for the treatment of type I Bartter syndrome.
26721884	1	59	theme	renal	168:172	arg1	NKCC2					208:212	NKCC2	208:212	NKCC2	208:212	Mutations in the renal specific Na-K-2Cl co-transporter (NKCC2) lead to type I Bartter syndrome, a life-threatening kidney disease featuring arterial hypotension along with electrolyte abnormalities.
26721884	1	59	theme	renal	168:172	arg1	co-transporter					192:205	the renal specific Na-K-2Cl co-transporter	164:205	the renal specific Na-K-2Cl co-transporter (NKCC2)	164:213	Mutations in the renal specific Na-K-2Cl co-transporter (NKCC2) lead to type I Bartter syndrome, a life-threatening kidney disease featuring arterial hypotension along with electrolyte abnormalities.
26721884	8	60	theme	proteasome	1160:1169	arg1	MG132					1181:1185	the proteasome inhibitor MG132	1156:1185	the proteasome inhibitor MG132	1156:1185	In cells overexpressing OS9, total cellular NKCC2 protein levels were markedly decreased, an effect blocked by the proteasome inhibitor MG132.
26721884	1	61	theme	Na-K-2Cl	183:190	arg1	NKCC2					208:212	NKCC2	208:212	NKCC2	208:212	Mutations in the renal specific Na-K-2Cl co-transporter (NKCC2) lead to type I Bartter syndrome, a life-threatening kidney disease featuring arterial hypotension along with electrolyte abnormalities.
26721884	1	61	theme	Na-K-2Cl	183:190	arg1	co-transporter					192:205	the renal specific Na-K-2Cl co-transporter	164:205	the renal specific Na-K-2Cl co-transporter (NKCC2)	164:213	Mutations in the renal specific Na-K-2Cl co-transporter (NKCC2) lead to type I Bartter syndrome, a life-threatening kidney disease featuring arterial hypotension along with electrolyte abnormalities.
26721884	0	62	theme	Reticulum-associated	109:128	arg1	Pathway					142:148	the Endoplasmic Reticulum-associated Degradation Pathway	93:148	the Endoplasmic Reticulum-associated Degradation Pathway	93:148	OS9 Protein Interacts with Na-K-2Cl Co-transporter (NKCC2) and Targets Its Immature Form for the Endoplasmic Reticulum-associated Degradation Pathway.
26721884	10	63	theme	small	1423:1427	arg1	RNA					1441:1443	small interfering RNA	1423:1443	small interfering RNA	1423:1443	Conversely, knockdown of OS9 by small interfering RNA increased NKCC2 expression by increasing the co-transporter stability.
26721884	14	64	dep	type	2093:2096	arg1	syndrome					2108:2115	Bartter syndrome	2100:2115	type I Bartter syndrome	2093:2115	The identification and selective modulation of ERAD components specific to NKCC2 and its disease-causing mutants might provide novel therapeutic strategies for the treatment of type I Bartter syndrome.
26721884	9	65	theme	marked	1253:1258	arg1	reduction					1260:1268	the marked reduction	1249:1268	the marked reduction in the co-transporter protein levels	1249:1305	Pulse-chase and cycloheximide-chase assays demonstrated that the marked reduction in the co-transporter protein levels was essentially due to increased protein degradation of the immature form of NKCC2.
26721884	9	65	theme	marked	1253:1258	arg1	due					1323:1325	due	1323:1325	due	1323:1325	Pulse-chase and cycloheximide-chase assays demonstrated that the marked reduction in the co-transporter protein levels was essentially due to increased protein degradation of the immature form of NKCC2.
26721884	4	66	theme	kidney	634:639	arg1	library					646:652	a kidney cDNA library	632:652	a kidney cDNA library	632:652	To this end, we screened a kidney cDNA library through a yeast two-hybrid assay using NKCC2 C terminus as bait.
26721884	5	67	dep	OS9	733:735	arg1	amplified					738:746	amplified	738:746	amplified in osteosarcomas	738:763	We identified OS9 (amplified in osteosarcomas) as a novel and specific binding partner of NKCC2.
26721884	4	68	theme	C	699:699	arg1	terminus					701:708	NKCC2 C terminus	693:708	NKCC2 C terminus	693:708	To this end, we screened a kidney cDNA library through a yeast two-hybrid assay using NKCC2 C terminus as bait.
26721884	7	69	theme	endoplasmic	1022:1032	arg1	reticulum					1034:1042	the endoplasmic reticulum	1018:1042	the endoplasmic reticulum	1018:1042	Accordingly, immunocytochemistry analysis showed that NKCC2 and OS9 co-localize at the endoplasmic reticulum.
26721884	9	70	theme	co-transporter	1277:1290	arg1	levels					1300:1305	the co-transporter protein levels	1273:1305	the co-transporter protein levels	1273:1305	Pulse-chase and cycloheximide-chase assays demonstrated that the marked reduction in the co-transporter protein levels was essentially due to increased protein degradation of the immature form of NKCC2.
26721884	12	71	theme	protein	1738:1744	arg1	degradation					1746:1756	OS9-induced protein degradation	1726:1756	OS9-induced protein degradation	1726:1756	In contrast, mutations of NKCC2 N-glycosylation sites abolished the effects of OS9, indicating that OS9-induced protein degradation is N-glycan-dependent.
26721884	14	72	theme	selective	1939:1947	arg1	modulation					1949:1958	selective modulation	1939:1958	selective modulation	1939:1958	The identification and selective modulation of ERAD components specific to NKCC2 and its disease-causing mutants might provide novel therapeutic strategies for the treatment of type I Bartter syndrome.
26721884	4	73	theme	two-hybrid	670:679	arg1	assay					681:685	a yeast two-hybrid assay	662:685	a yeast two-hybrid assay using NKCC2 C terminus as bait	662:716	To this end, we screened a kidney cDNA library through a yeast two-hybrid assay using NKCC2 C terminus as bait.
26721884	11	74	theme	homology	1565:1572	arg1	OS9					1584:1586	OS9	1584:1586	OS9	1584:1586	Inactivation of the mannose 6-phosphate receptor homology domain of OS9 had no effect on its action on NKCC2.
26721884	11	74	theme	homology	1565:1572	arg1	domain					1574:1579	the mannose 6-phosphate receptor homology domain	1532:1579	the mannose 6-phosphate receptor homology domain of OS9	1532:1586	Inactivation of the mannose 6-phosphate receptor homology domain of OS9 had no effect on its action on NKCC2.
26721884	11	75	theme	6-phosphate	1544:1554	arg1	receptor					1556:1563	mannose 6-phosphate receptor	1536:1563	the mannose 6-phosphate receptor homology domain of OS9	1532:1586	Inactivation of the mannose 6-phosphate receptor homology domain of OS9 had no effect on its action on NKCC2.
26721884	14	76	theme	type	2093:2096	arg1	treatment					2080:2088	the treatment	2076:2088	the treatment of type I Bartter syndrome	2076:2115	The identification and selective modulation of ERAD components specific to NKCC2 and its disease-causing mutants might provide novel therapeutic strategies for the treatment of type I Bartter syndrome.
26721884	10	77	theme	NKCC2	1455:1459	arg1	expression					1461:1470	NKCC2 expression	1455:1470	NKCC2 expression	1455:1470	Conversely, knockdown of OS9 by small interfering RNA increased NKCC2 expression by increasing the co-transporter stability.
26721884	13	78	theme	NKCC2	1900:1904	arg1	proteins					1906:1913	immature NKCC2 proteins	1891:1913	immature NKCC2 proteins	1891:1913	In summary, our results demonstrate the presence of an OS9-mediated ERAD pathway in renal cells that degrades immature NKCC2 proteins.
26721884	9	79	theme	protein	1340:1346	arg1	degradation					1348:1358	increased protein degradation	1330:1358	increased protein degradation of the immature form of NKCC2	1330:1388	Pulse-chase and cycloheximide-chase assays demonstrated that the marked reduction in the co-transporter protein levels was essentially due to increased protein degradation of the immature form of NKCC2.
26721884	1	80	from	Mutations	151:159	arg1	NKCC2					208:212	NKCC2	208:212	NKCC2	208:212	Mutations in the renal specific Na-K-2Cl co-transporter (NKCC2) lead to type I Bartter syndrome, a life-threatening kidney disease featuring arterial hypotension along with electrolyte abnormalities.
26721884	1	80	from	Mutations	151:159	arg1	co-transporter					192:205	the renal specific Na-K-2Cl co-transporter	164:205	the renal specific Na-K-2Cl co-transporter (NKCC2)	164:213	Mutations in the renal specific Na-K-2Cl co-transporter (NKCC2) lead to type I Bartter syndrome, a life-threatening kidney disease featuring arterial hypotension along with electrolyte abnormalities.
26721884	2	81	theme	disease-causing	395:409	arg1	mutants					411:417	its disease-causing mutants	391:417	its disease-causing mutants	391:417	We have previously shown that NKCC2 and its disease-causing mutants are subject to regulation by endoplasmic reticulum-associated degradation (ERAD).
26721884	2	82	theme	reticulum-associated	460:479	arg1	ERAD					494:497	ERAD	494:497	ERAD	494:497	We have previously shown that NKCC2 and its disease-causing mutants are subject to regulation by endoplasmic reticulum-associated degradation (ERAD).
26721884	2	82	theme	reticulum-associated	460:479	arg1	degradation					481:491	endoplasmic reticulum-associated degradation	448:491	endoplasmic reticulum-associated degradation (ERAD)	448:498	We have previously shown that NKCC2 and its disease-causing mutants are subject to regulation by endoplasmic reticulum-associated degradation (ERAD).
26721884	5	83	theme	binding	790:796	arg1	OS9					733:735	OS9	733:735	OS9 (amplified in osteosarcomas)	733:764	We identified OS9 (amplified in osteosarcomas) as a novel and specific binding partner of NKCC2.
26721884	5	83	theme	binding	790:796	arg1	partner					798:804	a novel and specific binding partner	769:804	a novel and specific binding partner of NKCC2	769:813	We identified OS9 (amplified in osteosarcomas) as a novel and specific binding partner of NKCC2.
26721884	9	84	theme	immature	1367:1374	arg1	form					1376:1379	the immature form	1363:1379	the immature form of NKCC2	1363:1388	Pulse-chase and cycloheximide-chase assays demonstrated that the marked reduction in the co-transporter protein levels was essentially due to increased protein degradation of the immature form of NKCC2.
26721884	8	85	theme	cellular	1080:1087	arg1	levels					1103:1108	total cellular NKCC2 protein levels	1074:1108	total cellular NKCC2 protein levels	1074:1108	In cells overexpressing OS9, total cellular NKCC2 protein levels were markedly decreased, an effect blocked by the proteasome inhibitor MG132.
26721884	14	86	theme	therapeutic	2049:2059	arg1	strategies					2061:2070	novel therapeutic strategies	2043:2070	novel therapeutic strategies	2043:2070	The identification and selective modulation of ERAD components specific to NKCC2 and its disease-causing mutants might provide novel therapeutic strategies for the treatment of type I Bartter syndrome.
26721884	12	87	theme	NKCC2	1652:1656	arg1	sites					1674:1678	NKCC2 N-glycosylation sites	1652:1678	NKCC2 N-glycosylation sites	1652:1678	In contrast, mutations of NKCC2 N-glycosylation sites abolished the effects of OS9, indicating that OS9-induced protein degradation is N-glycan-dependent.
26721884	1	88	theme	arterial	292:299	arg1	hypotension					301:311	arterial hypotension	292:311	arterial hypotension	292:311	Mutations in the renal specific Na-K-2Cl co-transporter (NKCC2) lead to type I Bartter syndrome, a life-threatening kidney disease featuring arterial hypotension along with electrolyte abnormalities.
26721884	8	89	theme	protein	1095:1101	arg1	levels					1103:1108	total cellular NKCC2 protein levels	1074:1108	total cellular NKCC2 protein levels	1074:1108	In cells overexpressing OS9, total cellular NKCC2 protein levels were markedly decreased, an effect blocked by the proteasome inhibitor MG132.
26721884	6	90	theme	renal	849:853	arg1	cells					855:859	renal cells	849:859	renal cells	849:859	Co-immunoprecipitation assays in renal cells revealed that OS9 association involves mainly the immature form of NKCC2.
26721884	12	91	theme	sites	1674:1678	arg1	mutations					1639:1647	mutations	1639:1647	mutations of NKCC2 N-glycosylation sites	1639:1678	In contrast, mutations of NKCC2 N-glycosylation sites abolished the effects of OS9, indicating that OS9-induced protein degradation is N-glycan-dependent.
26721884	12	92	gly	N-glycosylation	1658:1672	arg2	sites					1674:1678	NKCC2 N-glycosylation sites	1652:1678	NKCC2 N-glycosylation sites	1652:1678	In contrast, mutations of NKCC2 N-glycosylation sites abolished the effects of OS9, indicating that OS9-induced protein degradation is N-glycan-dependent.
26721884	1	93	theme	electrolyte	324:334	arg1	abnormalities					336:348	electrolyte abnormalities	324:348	electrolyte abnormalities	324:348	Mutations in the renal specific Na-K-2Cl co-transporter (NKCC2) lead to type I Bartter syndrome, a life-threatening kidney disease featuring arterial hypotension along with electrolyte abnormalities.
26721884	1	94	theme	type	223:226	arg1	disease					274:280	a life-threatening kidney disease	248:280	a life-threatening kidney disease featuring arterial hypotension along with electrolyte abnormalities	248:348	Mutations in the renal specific Na-K-2Cl co-transporter (NKCC2) lead to type I Bartter syndrome, a life-threatening kidney disease featuring arterial hypotension along with electrolyte abnormalities.
26721884	1	94	theme	type	223:226	arg1	syndrome					238:245	type I Bartter syndrome	223:245	type I Bartter syndrome	223:245	Mutations in the renal specific Na-K-2Cl co-transporter (NKCC2) lead to type I Bartter syndrome, a life-threatening kidney disease featuring arterial hypotension along with electrolyte abnormalities.
26721884	14	95	dep	identification	1920:1933	arg1	The					1916:1918	The	1916:1918	The	1916:1918	The identification and selective modulation of ERAD components specific to NKCC2 and its disease-causing mutants might provide novel therapeutic strategies for the treatment of type I Bartter syndrome.
26721884	13	96	theme	pathway	1854:1860	arg1	presence					1821:1828	the presence	1817:1828	the presence of an OS9-mediated ERAD pathway in renal cells that degrades immature NKCC2 proteins	1817:1913	In summary, our results demonstrate the presence of an OS9-mediated ERAD pathway in renal cells that degrades immature NKCC2 proteins.
26721884	1	97	theme	Bartter	230:236	arg1	disease					274:280	a life-threatening kidney disease	248:280	a life-threatening kidney disease featuring arterial hypotension along with electrolyte abnormalities	248:348	Mutations in the renal specific Na-K-2Cl co-transporter (NKCC2) lead to type I Bartter syndrome, a life-threatening kidney disease featuring arterial hypotension along with electrolyte abnormalities.
26721884	1	97	theme	Bartter	230:236	arg1	syndrome					238:245	type I Bartter syndrome	223:245	type I Bartter syndrome	223:245	Mutations in the renal specific Na-K-2Cl co-transporter (NKCC2) lead to type I Bartter syndrome, a life-threatening kidney disease featuring arterial hypotension along with electrolyte abnormalities.
28514686	0	0	theme	Stabilized	96:105	arg1	Glycoprotein					130:141	a Stabilized Subtype C HIV Envelope Glycoprotein	94:141	a Stabilized Subtype C HIV Envelope Glycoprotein	94:141	Glycine Substitution at Helix-to-Coil Transitions Facilitates the Structural Determination of a Stabilized Subtype C HIV Envelope Glycoprotein.
28514686	1	1	theme	glycoprotein	171:182	arg1	design					190:195	HIV-1 envelope glycoprotein (Env) design	156:195	HIV-1 envelope glycoprotein (Env) design	156:195	Advances in HIV-1 envelope glycoprotein (Env) design generate native-like trimers and high-resolution clade A, B, and G structures and elicit neutralizing antibodies.
28514686	6	2	theme	homogeneous	1120:1130	arg1	immunogens					1144:1153	multiple homogeneous cross-clade immunogens	1111:1153	multiple homogeneous cross-clade immunogens with the potential to advance HIV vaccine development	1111:1207	Env-structure-guided design strategies resulted in multiple homogeneous cross-clade immunogens with the potential to advance HIV vaccine development.
28514686	0	3	theme	C	115:115	arg1	Glycoprotein					130:141	a Stabilized Subtype C HIV Envelope Glycoprotein	94:141	a Stabilized Subtype C HIV Envelope Glycoprotein	94:141	Glycine Substitution at Helix-to-Coil Transitions Facilitates the Structural Determination of a Stabilized Subtype C HIV Envelope Glycoprotein.
28514686	3	4	theme	post-fusion	680:690	arg1	conformations					692:704	post-fusion conformations	680:704	post-fusion conformations	680:704	Based on targeted glycine substitutions in the Env fusion machinery, we defined a general approach that disfavors helical transitions leading to post-fusion conformations, thereby favoring the pre-fusion state.
28514686	4	5	theme	stabilized	761:770	arg1	Env					789:791	soluble clade C Env	773:791	soluble clade C Env (16055 NFL)	773:803	We generated a stabilized, soluble clade C Env (16055 NFL) and determined its crystal structure at 3.9 Å.
28514686	4	5	theme	stabilized	761:770	arg1	NFL					800:802	16055 NFL	794:802	16055 NFL	794:802	We generated a stabilized, soluble clade C Env (16055 NFL) and determined its crystal structure at 3.9 Å.
28514686	1	6	theme	G	262:262	arg1	structures					264:273	high-resolution clade A, B, and G structures	230:273	high-resolution clade A, B, and G structures	230:273	Advances in HIV-1 envelope glycoprotein (Env) design generate native-like trimers and high-resolution clade A, B, and G structures and elicit neutralizing antibodies.
28514686	0	7	theme	Subtype	107:113	arg1	Glycoprotein					130:141	a Stabilized Subtype C HIV Envelope Glycoprotein	94:141	a Stabilized Subtype C HIV Envelope Glycoprotein	94:141	Glycine Substitution at Helix-to-Coil Transitions Facilitates the Structural Determination of a Stabilized Subtype C HIV Envelope Glycoprotein.
28514686	6	8	theme	HIV	1185:1187	arg1	development					1197:1207	HIV vaccine development	1185:1207	HIV vaccine development	1185:1207	Env-structure-guided design strategies resulted in multiple homogeneous cross-clade immunogens with the potential to advance HIV vaccine development.
28514686	5	9	theme	Env	912:914	arg1	trimers					916:922	native Env trimers	905:922	native Env trimers	905:922	Its overall conformation is similar to SOSIP.664 and native Env trimers but includes a covalent linker between gp120 and gp41, an engineered 201-433 disulfide bond, and density corresponding to 22 N-glycans.
28514686	0	10	theme	Envelope	121:128	arg1	Glycoprotein					130:141	a Stabilized Subtype C HIV Envelope Glycoprotein	94:141	a Stabilized Subtype C HIV Envelope Glycoprotein	94:141	Glycine Substitution at Helix-to-Coil Transitions Facilitates the Structural Determination of a Stabilized Subtype C HIV Envelope Glycoprotein.
28514686	3	11	theme	pre-fusion	728:737	arg1	state					739:743	the pre-fusion state	724:743	the pre-fusion state	724:743	Based on targeted glycine substitutions in the Env fusion machinery, we defined a general approach that disfavors helical transitions leading to post-fusion conformations, thereby favoring the pre-fusion state.
28514686	6	12	theme	design	1081:1086	arg1	strategies					1088:1097	Env-structure-guided design strategies	1060:1097	Env-structure-guided design strategies	1060:1097	Env-structure-guided design strategies resulted in multiple homogeneous cross-clade immunogens with the potential to advance HIV vaccine development.
28514686	4	13	theme	soluble	773:779	arg1	Env					789:791	soluble clade C Env	773:791	soluble clade C Env (16055 NFL)	773:803	We generated a stabilized, soluble clade C Env (16055 NFL) and determined its crystal structure at 3.9 Å.
28514686	4	13	theme	soluble	773:779	arg1	NFL					800:802	16055 NFL	794:802	16055 NFL	794:802	We generated a stabilized, soluble clade C Env (16055 NFL) and determined its crystal structure at 3.9 Å.
28514686	5	14	theme	native	905:910	arg1	trimers					916:922	native Env trimers	905:922	native Env trimers	905:922	Its overall conformation is similar to SOSIP.664 and native Env trimers but includes a covalent linker between gp120 and gp41, an engineered 201-433 disulfide bond, and density corresponding to 22 N-glycans.
28514686	0	15	theme	HIV	117:119	arg1	Glycoprotein					130:141	a Stabilized Subtype C HIV Envelope Glycoprotein	94:141	a Stabilized Subtype C HIV Envelope Glycoprotein	94:141	Glycine Substitution at Helix-to-Coil Transitions Facilitates the Structural Determination of a Stabilized Subtype C HIV Envelope Glycoprotein.
28514686	6	16	theme	multiple	1111:1118	arg1	immunogens					1144:1153	multiple homogeneous cross-clade immunogens	1111:1153	multiple homogeneous cross-clade immunogens with the potential to advance HIV vaccine development	1111:1207	Env-structure-guided design strategies resulted in multiple homogeneous cross-clade immunogens with the potential to advance HIV vaccine development.
28514686	1	17	from	Advances	144:151	arg1	design					190:195	HIV-1 envelope glycoprotein (Env) design	156:195	HIV-1 envelope glycoprotein (Env) design	156:195	Advances in HIV-1 envelope glycoprotein (Env) design generate native-like trimers and high-resolution clade A, B, and G structures and elicit neutralizing antibodies.
28514686	6	18	theme	Env-structure-guided	1060:1079	arg1	strategies					1088:1097	Env-structure-guided design strategies	1060:1097	Env-structure-guided design strategies	1060:1097	Env-structure-guided design strategies resulted in multiple homogeneous cross-clade immunogens with the potential to advance HIV vaccine development.
28514686	5	19	theme	201-433	993:999	arg1	bond					1011:1014	an engineered 201-433 disulfide bond	979:1014	an engineered 201-433 disulfide bond	979:1014	Its overall conformation is similar to SOSIP.664 and native Env trimers but includes a covalent linker between gp120 and gp41, an engineered 201-433 disulfide bond, and density corresponding to 22 N-glycans.
28514686	0	20	from	Transitions	38:48	arg1	Substitution					8:19	Glycine Substitution	0:19	Glycine Substitution at Helix-to-Coil Transitions	0:48	Glycine Substitution at Helix-to-Coil Transitions Facilitates the Structural Determination of a Stabilized Subtype C HIV Envelope Glycoprotein.
28514686	3	21	theme	glycine	553:559	arg1	substitutions					561:573	targeted glycine substitutions	544:573	targeted glycine substitutions in the Env fusion machinery	544:601	Based on targeted glycine substitutions in the Env fusion machinery, we defined a general approach that disfavors helical transitions leading to post-fusion conformations, thereby favoring the pre-fusion state.
28514686	0	22	theme	Glycine	0:6	arg1	Substitution					8:19	Glycine Substitution	0:19	Glycine Substitution at Helix-to-Coil Transitions	0:48	Glycine Substitution at Helix-to-Coil Transitions Facilitates the Structural Determination of a Stabilized Subtype C HIV Envelope Glycoprotein.
28514686	4	23	theme	crystal	824:830	arg1	structure					832:840	its crystal structure at 3.9 Å	820:849	its crystal structure at 3.9 Å	820:849	We generated a stabilized, soluble clade C Env (16055 NFL) and determined its crystal structure at 3.9 Å.
28514686	5	24	theme	overall	856:862	arg1	conformation					864:875	Its overall conformation	852:875	Its overall conformation	852:875	Its overall conformation is similar to SOSIP.664 and native Env trimers but includes a covalent linker between gp120 and gp41, an engineered 201-433 disulfide bond, and density corresponding to 22 N-glycans.
28514686	0	25	theme	Helix-to-Coil	24:36	arg1	Transitions					38:48	Helix-to-Coil Transitions	24:48	Helix-to-Coil Transitions	24:48	Glycine Substitution at Helix-to-Coil Transitions Facilitates the Structural Determination of a Stabilized Subtype C HIV Envelope Glycoprotein.
28514686	5	26	theme	engineered	982:991	arg1	bond					1011:1014	an engineered 201-433 disulfide bond	979:1014	an engineered 201-433 disulfide bond	979:1014	Its overall conformation is similar to SOSIP.664 and native Env trimers but includes a covalent linker between gp120 and gp41, an engineered 201-433 disulfide bond, and density corresponding to 22 N-glycans.
28514686	1	27	theme	native-like	206:216	arg1	trimers					218:224	native-like trimers	206:224	native-like trimers	206:224	Advances in HIV-1 envelope glycoprotein (Env) design generate native-like trimers and high-resolution clade A, B, and G structures and elicit neutralizing antibodies.
28514686	2	28	theme	infections	418:427	arg1	worldwide					429:437	HIV infections worldwide	414:437	HIV infections worldwide	414:437	However, a high-resolution clade C structure is critical, as this subtype accounts for the majority of HIV infections worldwide, but well-ordered clade C Env trimers are more challenging to produce due to their instability.
28514686	1	29	theme	neutralizing	286:297	arg1	antibodies					299:308	neutralizing antibodies	286:308	neutralizing antibodies	286:308	Advances in HIV-1 envelope glycoprotein (Env) design generate native-like trimers and high-resolution clade A, B, and G structures and elicit neutralizing antibodies.
28514686	0	30	theme	Glycoprotein	130:141	arg1	Determination					77:89	the Structural Determination	62:89	the Structural Determination of a Stabilized Subtype C HIV Envelope Glycoprotein	62:141	Glycine Substitution at Helix-to-Coil Transitions Facilitates the Structural Determination of a Stabilized Subtype C HIV Envelope Glycoprotein.
28514686	6	31	theme	vaccine	1189:1195	arg1	development					1197:1207	HIV vaccine development	1185:1207	HIV vaccine development	1185:1207	Env-structure-guided design strategies resulted in multiple homogeneous cross-clade immunogens with the potential to advance HIV vaccine development.
28514686	3	32	theme	targeted	544:551	arg1	substitutions					561:573	targeted glycine substitutions	544:573	targeted glycine substitutions in the Env fusion machinery	544:601	Based on targeted glycine substitutions in the Env fusion machinery, we defined a general approach that disfavors helical transitions leading to post-fusion conformations, thereby favoring the pre-fusion state.
28514686	2	33	theme	Env	465:467	arg1	trimers					469:475	well-ordered clade C Env trimers	444:475	well-ordered clade C Env trimers	444:475	However, a high-resolution clade C structure is critical, as this subtype accounts for the majority of HIV infections worldwide, but well-ordered clade C Env trimers are more challenging to produce due to their instability.
28514686	5	34	theme	covalent	939:946	arg1	linker					948:953	a covalent linker	937:953	a covalent linker between gp120 and gp41, an engineered 201-433 disulfide bond, and density corresponding to 22 N-glycans	937:1057	Its overall conformation is similar to SOSIP.664 and native Env trimers but includes a covalent linker between gp120 and gp41, an engineered 201-433 disulfide bond, and density corresponding to 22 N-glycans.
28514686	3	35	theme	Env	582:584	arg1	machinery					593:601	the Env fusion machinery	578:601	the Env fusion machinery	578:601	Based on targeted glycine substitutions in the Env fusion machinery, we defined a general approach that disfavors helical transitions leading to post-fusion conformations, thereby favoring the pre-fusion state.
28514686	2	36	theme	C	463:463	arg1	trimers					469:475	well-ordered clade C Env trimers	444:475	well-ordered clade C Env trimers	444:475	However, a high-resolution clade C structure is critical, as this subtype accounts for the majority of HIV infections worldwide, but well-ordered clade C Env trimers are more challenging to produce due to their instability.
28514686	2	37	theme	worldwide	429:437	arg1	majority					402:409	the majority	398:409	the majority of HIV infections worldwide	398:437	However, a high-resolution clade C structure is critical, as this subtype accounts for the majority of HIV infections worldwide, but well-ordered clade C Env trimers are more challenging to produce due to their instability.
28514686	1	38	theme	high-resolution	230:244	arg1	structures					264:273	high-resolution clade A, B, and G structures	230:273	high-resolution clade A, B, and G structures	230:273	Advances in HIV-1 envelope glycoprotein (Env) design generate native-like trimers and high-resolution clade A, B, and G structures and elicit neutralizing antibodies.
28514686	0	39	theme	Structural	66:75	arg1	Determination					77:89	the Structural Determination	62:89	the Structural Determination of a Stabilized Subtype C HIV Envelope Glycoprotein	62:141	Glycine Substitution at Helix-to-Coil Transitions Facilitates the Structural Determination of a Stabilized Subtype C HIV Envelope Glycoprotein.
28514686	6	40	with	immunogens	1144:1153	arg1	potential					1164:1172	the potential to advance HIV vaccine development	1160:1207	the potential to advance HIV vaccine development	1160:1207	Env-structure-guided design strategies resulted in multiple homogeneous cross-clade immunogens with the potential to advance HIV vaccine development.
28514686	3	41	theme	general	617:623	arg1	approach					625:632	a general approach	615:632	a general approach that disfavors helical transitions leading to post-fusion conformations, thereby favoring the pre-fusion state	615:743	Based on targeted glycine substitutions in the Env fusion machinery, we defined a general approach that disfavors helical transitions leading to post-fusion conformations, thereby favoring the pre-fusion state.
28514686	5	42	theme	disulfide	1001:1009	arg1	bond					1011:1014	an engineered 201-433 disulfide bond	979:1014	an engineered 201-433 disulfide bond	979:1014	Its overall conformation is similar to SOSIP.664 and native Env trimers but includes a covalent linker between gp120 and gp41, an engineered 201-433 disulfide bond, and density corresponding to 22 N-glycans.
28514686	6	43	theme	cross-clade	1132:1142	arg1	immunogens					1144:1153	multiple homogeneous cross-clade immunogens	1111:1153	multiple homogeneous cross-clade immunogens with the potential to advance HIV vaccine development	1111:1207	Env-structure-guided design strategies resulted in multiple homogeneous cross-clade immunogens with the potential to advance HIV vaccine development.
28514686	2	44	theme	well-ordered	444:455	arg1	trimers					469:475	well-ordered clade C Env trimers	444:475	well-ordered clade C Env trimers	444:475	However, a high-resolution clade C structure is critical, as this subtype accounts for the majority of HIV infections worldwide, but well-ordered clade C Env trimers are more challenging to produce due to their instability.
28514686	2	45	theme	C	344:344	arg1	critical					359:366	critical	359:366	critical	359:366	However, a high-resolution clade C structure is critical, as this subtype accounts for the majority of HIV infections worldwide, but well-ordered clade C Env trimers are more challenging to produce due to their instability.
28514686	2	45	theme	C	344:344	arg1	structure					346:354	a high-resolution clade C structure	320:354	a high-resolution clade C structure	320:354	However, a high-resolution clade C structure is critical, as this subtype accounts for the majority of HIV infections worldwide, but well-ordered clade C Env trimers are more challenging to produce due to their instability.
28514686	4	46	theme	C	787:787	arg1	Env					789:791	soluble clade C Env	773:791	soluble clade C Env (16055 NFL)	773:803	We generated a stabilized, soluble clade C Env (16055 NFL) and determined its crystal structure at 3.9 Å.
28514686	4	46	theme	C	787:787	arg1	NFL					800:802	16055 NFL	794:802	16055 NFL	794:802	We generated a stabilized, soluble clade C Env (16055 NFL) and determined its crystal structure at 3.9 Å.
28514686	3	47	theme	fusion	586:591	arg1	machinery					593:601	the Env fusion machinery	578:601	the Env fusion machinery	578:601	Based on targeted glycine substitutions in the Env fusion machinery, we defined a general approach that disfavors helical transitions leading to post-fusion conformations, thereby favoring the pre-fusion state.
28514686	2	48	theme	clade	338:342	arg1	critical					359:366	critical	359:366	critical	359:366	However, a high-resolution clade C structure is critical, as this subtype accounts for the majority of HIV infections worldwide, but well-ordered clade C Env trimers are more challenging to produce due to their instability.
28514686	2	48	theme	clade	338:342	arg1	structure					346:354	a high-resolution clade C structure	320:354	a high-resolution clade C structure	320:354	However, a high-resolution clade C structure is critical, as this subtype accounts for the majority of HIV infections worldwide, but well-ordered clade C Env trimers are more challenging to produce due to their instability.
28514686	1	49	theme	clade	246:250	arg1	A					252:252	clade A	246:252	clade A	246:252	Advances in HIV-1 envelope glycoprotein (Env) design generate native-like trimers and high-resolution clade A, B, and G structures and elicit neutralizing antibodies.
28514686	1	50	gly	glycoprotein	171:182	arg1	Env					185:187	Env	185:187	Env	185:187	Advances in HIV-1 envelope glycoprotein (Env) design generate native-like trimers and high-resolution clade A, B, and G structures and elicit neutralizing antibodies.
28514686	1	50	gly	glycoprotein	171:182	arg1	glycoprotein					171:182	HIV-1 envelope glycoprotein	156:182	HIV-1 envelope glycoprotein (Env) design	156:195	Advances in HIV-1 envelope glycoprotein (Env) design generate native-like trimers and high-resolution clade A, B, and G structures and elicit neutralizing antibodies.
28514686	4	51	theme	clade	781:785	arg1	Env					789:791	soluble clade C Env	773:791	soluble clade C Env (16055 NFL)	773:803	We generated a stabilized, soluble clade C Env (16055 NFL) and determined its crystal structure at 3.9 Å.
28514686	4	51	theme	clade	781:785	arg1	NFL					800:802	16055 NFL	794:802	16055 NFL	794:802	We generated a stabilized, soluble clade C Env (16055 NFL) and determined its crystal structure at 3.9 Å.
28514686	2	52	theme	high-resolution	322:336	arg1	critical					359:366	critical	359:366	critical	359:366	However, a high-resolution clade C structure is critical, as this subtype accounts for the majority of HIV infections worldwide, but well-ordered clade C Env trimers are more challenging to produce due to their instability.
28514686	2	52	theme	high-resolution	322:336	arg1	structure					346:354	a high-resolution clade C structure	320:354	a high-resolution clade C structure	320:354	However, a high-resolution clade C structure is critical, as this subtype accounts for the majority of HIV infections worldwide, but well-ordered clade C Env trimers are more challenging to produce due to their instability.
28514686	1	53	theme	A	252:252	arg1	structures					264:273	high-resolution clade A, B, and G structures	230:273	high-resolution clade A, B, and G structures	230:273	Advances in HIV-1 envelope glycoprotein (Env) design generate native-like trimers and high-resolution clade A, B, and G structures and elicit neutralizing antibodies.
28514686	3	54	theme	helical	649:655	arg1	transitions					657:667	helical transitions	649:667	helical transitions leading to post-fusion conformations	649:704	Based on targeted glycine substitutions in the Env fusion machinery, we defined a general approach that disfavors helical transitions leading to post-fusion conformations, thereby favoring the pre-fusion state.
28514686	2	55	theme	HIV	414:416	arg1	worldwide					429:437	HIV infections worldwide	414:437	HIV infections worldwide	414:437	However, a high-resolution clade C structure is critical, as this subtype accounts for the majority of HIV infections worldwide, but well-ordered clade C Env trimers are more challenging to produce due to their instability.
28514686	1	56	theme	HIV-1	156:160	arg1	Env					185:187	Env	185:187	Env	185:187	Advances in HIV-1 envelope glycoprotein (Env) design generate native-like trimers and high-resolution clade A, B, and G structures and elicit neutralizing antibodies.
28514686	1	56	theme	HIV-1	156:160	arg1	glycoprotein					171:182	HIV-1 envelope glycoprotein	156:182	HIV-1 envelope glycoprotein (Env) design	156:195	Advances in HIV-1 envelope glycoprotein (Env) design generate native-like trimers and high-resolution clade A, B, and G structures and elicit neutralizing antibodies.
28514686	3	57	from	substitutions	561:573	arg1	machinery					593:601	the Env fusion machinery	578:601	the Env fusion machinery	578:601	Based on targeted glycine substitutions in the Env fusion machinery, we defined a general approach that disfavors helical transitions leading to post-fusion conformations, thereby favoring the pre-fusion state.
28514686	2	58	theme	clade	457:461	arg1	trimers					469:475	well-ordered clade C Env trimers	444:475	well-ordered clade C Env trimers	444:475	However, a high-resolution clade C structure is critical, as this subtype accounts for the majority of HIV infections worldwide, but well-ordered clade C Env trimers are more challenging to produce due to their instability.
28514686	1	59	theme	envelope	162:169	arg1	Env					185:187	Env	185:187	Env	185:187	Advances in HIV-1 envelope glycoprotein (Env) design generate native-like trimers and high-resolution clade A, B, and G structures and elicit neutralizing antibodies.
28514686	1	59	theme	envelope	162:169	arg1	glycoprotein					171:182	HIV-1 envelope glycoprotein	156:182	HIV-1 envelope glycoprotein (Env) design	156:195	Advances in HIV-1 envelope glycoprotein (Env) design generate native-like trimers and high-resolution clade A, B, and G structures and elicit neutralizing antibodies.
28514686	1	60	theme	B	255:255	arg1	structures					264:273	high-resolution clade A, B, and G structures	230:273	high-resolution clade A, B, and G structures	230:273	Advances in HIV-1 envelope glycoprotein (Env) design generate native-like trimers and high-resolution clade A, B, and G structures and elicit neutralizing antibodies.
26553286	0	0	theme	binding	88:94	arg1	inhibition					67:76	inhibition	67:76	inhibition of lectin binding by glycoclusters in the natural tissue context	67:141	Merging carbohydrate chemistry with lectin histochemistry to study inhibition of lectin binding by glycoclusters in the natural tissue context.
26553286	6	1	from	changes	960:966	arg1	α/β					994:996	α/β	994:996	α/β	994:996	Besides valency, this panel covers changes in the anomeric position (α/β) and the atom at the glycosidic linkage (O/S).
26553286	6	1	from	changes	960:966	arg1	position					984:991	the anomeric position	971:991	the anomeric position (α/β)	971:997	Besides valency, this panel covers changes in the anomeric position (α/β) and the atom at the glycosidic linkage (O/S).
26553286	1	2	theme	glycans	159:165	arg1	Recognition					144:154	Recognition	144:154	Recognition of glycans by lectins	144:176	Recognition of glycans by lectins leads to cell adhesion and growth regulation.
26553286	4	3	from	that	681:684	arg1	different					666:674	different	666:674	different	666:674	The drawback of these assays is that glycan presentation is different from that in tissues.
26553286	0	4	theme	lectin	81:86	arg1	binding					88:94	lectin binding	81:94	lectin binding	81:94	Merging carbohydrate chemistry with lectin histochemistry to study inhibition of lectin binding by glycoclusters in the natural tissue context.
26553286	8	5	theme	method	1414:1419	arg1	applicability					1392:1404	the applicability	1388:1404	the applicability of this method	1388:1419	The marked and differential sensitivity of signal generation to structural aspects of the glycoclusters proves the applicability of this method.
26553286	11	6	from	glycoclusters	1712:1724	arg1	histochemistry					1736:1749	lectin histochemistry	1729:1749	lectin histochemistry	1729:1749	Testing glycoclusters in lectin histochemistry will especially be attractive in cases of multi-target recognition (glycans, proteins and lipids) by a tissue lectin.
26553286	11	7	from	cases	1784:1788	arg1	attractive					1770:1779	attractive	1770:1779	attractive	1770:1779	Testing glycoclusters in lectin histochemistry will especially be attractive in cases of multi-target recognition (glycans, proteins and lipids) by a tissue lectin.
26553286	7	8	theme	stomach	1214:1220	arg1	sections					1186:1193	sections	1186:1193	sections of murine jejunum, stomach and epididymis	1186:1235	Flanked by cell and solid-phase assays with human tumor lines and two mucins, respectively, staining (intensity and profile) was analyzed in sections of murine jejunum, stomach and epididymis as a function of glycocluster presence.
26553286	2	9	theme	carbohydrate	290:301	arg1	structure					303:311	carbohydrate structure	290:311	carbohydrate structure (sequence and shape)	290:332	The specificity and selectivity of this process are determined by carbohydrate structure (sequence and shape) and topology of its presentation.
26553286	9	10	theme	data	1455:1458	arg1	sets					1460:1463	data sets	1455:1463	data sets obtained by using (neo)glycoconjugates, cells and the tissue context as platforms	1455:1545	This enables comparisons between data sets obtained by using (neo)glycoconjugates, cells and the tissue context as platforms.
26553286	6	11	from	atom	1007:1010	arg1	α/β					994:996	α/β	994:996	α/β	994:996	Besides valency, this panel covers changes in the anomeric position (α/β) and the atom at the glycosidic linkage (O/S).
26553286	6	11	from	atom	1007:1010	arg1	position					984:991	the anomeric position	971:991	the anomeric position (α/β)	971:997	Besides valency, this panel covers changes in the anomeric position (α/β) and the atom at the glycosidic linkage (O/S).
26553286	5	12	theme	glycoclusters	910:922	arg1	series					897:902	a series	895:902	a series of 10 glycoclusters	895:922	In order to approach the natural context, we here introduce lectin histochemistry on fixed tissue sections to determine the susceptibility of binding of two plant lectins, i.e., GSA-II and WGA, to a series of 10 glycoclusters.
26553286	5	13	theme	binding	840:846	arg1	susceptibility					822:835	the susceptibility	818:835	the susceptibility of binding of two plant lectins, i.e., GSA-II and WGA, to a series of 10 glycoclusters	818:922	In order to approach the natural context, we here introduce lectin histochemistry on fixed tissue sections to determine the susceptibility of binding of two plant lectins, i.e., GSA-II and WGA, to a series of 10 glycoclusters.
26553286	7	14	theme	tumor	1095:1099	arg1	lines					1101:1105	human tumor lines	1089:1105	human tumor lines	1089:1105	Flanked by cell and solid-phase assays with human tumor lines and two mucins, respectively, staining (intensity and profile) was analyzed in sections of murine jejunum, stomach and epididymis as a function of glycocluster presence.
26553286	3	15	theme	structure-activity	475:492	arg1	relationships					494:506	structure-activity relationships	475:506	structure-activity relationships	475:506	The synthesis of (neo)glycoconjugates with bi- to oligo-valency (glycoclusters) affords tools to delineate structure-activity relationships by blocking lectin binding to an artificial matrix, often a glycoprotein, or cultured cell lines.
26553286	5	16	theme	fixed	783:787	arg1	sections					796:803	fixed tissue sections	783:803	fixed tissue sections	783:803	In order to approach the natural context, we here introduce lectin histochemistry on fixed tissue sections to determine the susceptibility of binding of two plant lectins, i.e., GSA-II and WGA, to a series of 10 glycoclusters.
26553286	10	17	theme	association	1646:1656	arg1	sites					1661:1665	lectin association at sites	1639:1665	lectin association at sites that are relevant for functionality	1639:1701	The special advantage of processing tissue sections is the monitoring of interference with lectin association at sites that are relevant for functionality.
26553286	4	18	theme	glycan	643:648	arg1	presentation					650:661	glycan presentation	643:661	glycan presentation	643:661	The drawback of these assays is that glycan presentation is different from that in tissues.
26553286	5	19	theme	natural	723:729	arg1	context					731:737	the natural context	719:737	the natural context	719:737	In order to approach the natural context, we here introduce lectin histochemistry on fixed tissue sections to determine the susceptibility of binding of two plant lectins, i.e., GSA-II and WGA, to a series of 10 glycoclusters.
26553286	7	20	theme	cell	1056:1059	arg1	assays					1077:1082	cell and solid-phase assays	1056:1082	assays	1077:1082	Flanked by cell and solid-phase assays with human tumor lines and two mucins, respectively, staining (intensity and profile) was analyzed in sections of murine jejunum, stomach and epididymis as a function of glycocluster presence.
26553286	7	21	theme	murine	1198:1203	arg1	jejunum					1205:1211	murine jejunum	1198:1211	murine jejunum	1198:1211	Flanked by cell and solid-phase assays with human tumor lines and two mucins, respectively, staining (intensity and profile) was analyzed in sections of murine jejunum, stomach and epididymis as a function of glycocluster presence.
26553286	5	22	theme	tissue	789:794	arg1	sections					796:803	fixed tissue sections	783:803	fixed tissue sections	783:803	In order to approach the natural context, we here introduce lectin histochemistry on fixed tissue sections to determine the susceptibility of binding of two plant lectins, i.e., GSA-II and WGA, to a series of 10 glycoclusters.
26553286	8	23	theme	marked	1281:1286	arg1	sensitivity					1305:1315	The marked and differential sensitivity	1277:1315	The marked and differential sensitivity of signal generation to structural aspects of the glycoclusters	1277:1379	The marked and differential sensitivity of signal generation to structural aspects of the glycoclusters proves the applicability of this method.
26553286	0	24	theme	carbohydrate	8:19	arg1	chemistry					21:29	Merging carbohydrate chemistry	0:29	Merging carbohydrate chemistry with lectin histochemistry	0:56	Merging carbohydrate chemistry with lectin histochemistry to study inhibition of lectin binding by glycoclusters in the natural tissue context.
26553286	11	25	from	attractive	1770:1779	arg1	cases					1784:1788	cases	1784:1788	cases of multi-target recognition (glycans, proteins and lipids) by a tissue lectin	1784:1866	Testing glycoclusters in lectin histochemistry will especially be attractive in cases of multi-target recognition (glycans, proteins and lipids) by a tissue lectin.
26553286	7	26	dep	staining	1137:1144	arg1	intensity					1147:1155	intensity	1147:1155	intensity	1147:1155	Flanked by cell and solid-phase assays with human tumor lines and two mucins, respectively, staining (intensity and profile) was analyzed in sections of murine jejunum, stomach and epididymis as a function of glycocluster presence.
26553286	7	26	dep	staining	1137:1144	arg1	profile					1161:1167	profile	1161:1167	profile	1161:1167	Flanked by cell and solid-phase assays with human tumor lines and two mucins, respectively, staining (intensity and profile) was analyzed in sections of murine jejunum, stomach and epididymis as a function of glycocluster presence.
26553286	7	27	theme	jejunum	1205:1211	arg1	sections					1186:1193	sections	1186:1193	sections of murine jejunum, stomach and epididymis	1186:1235	Flanked by cell and solid-phase assays with human tumor lines and two mucins, respectively, staining (intensity and profile) was analyzed in sections of murine jejunum, stomach and epididymis as a function of glycocluster presence.
26553286	0	28	theme	Merging	0:6	arg1	chemistry					21:29	Merging carbohydrate chemistry	0:29	Merging carbohydrate chemistry with lectin histochemistry	0:56	Merging carbohydrate chemistry with lectin histochemistry to study inhibition of lectin binding by glycoclusters in the natural tissue context.
26553286	11	29	theme	Testing	1704:1710	arg1	glycoclusters					1712:1724	Testing glycoclusters	1704:1724	Testing glycoclusters in lectin histochemistry	1704:1749	Testing glycoclusters in lectin histochemistry will especially be attractive in cases of multi-target recognition (glycans, proteins and lipids) by a tissue lectin.
26553286	10	30	with	interference	1621:1632	arg1	sites					1661:1665	lectin association at sites	1639:1665	lectin association at sites that are relevant for functionality	1639:1701	The special advantage of processing tissue sections is the monitoring of interference with lectin association at sites that are relevant for functionality.
26553286	10	31	theme	tissue	1584:1589	arg1	sections					1591:1598	processing tissue sections	1573:1598	processing tissue sections	1573:1598	The special advantage of processing tissue sections is the monitoring of interference with lectin association at sites that are relevant for functionality.
26553286	3	32	with	synthesis	372:380	arg1	bi-					411:413	bi-	411:413	bi- to oligo-valency (glycoclusters)	411:446	The synthesis of (neo)glycoconjugates with bi- to oligo-valency (glycoclusters) affords tools to delineate structure-activity relationships by blocking lectin binding to an artificial matrix, often a glycoprotein, or cultured cell lines.
26553286	1	33	theme	cell	187:190	arg1	adhesion					192:199	cell adhesion	187:199	cell adhesion	187:199	Recognition of glycans by lectins leads to cell adhesion and growth regulation.
26553286	7	34	theme	solid-phase	1065:1075	arg1	assays					1077:1082	cell and solid-phase assays	1056:1082	assays	1077:1082	Flanked by cell and solid-phase assays with human tumor lines and two mucins, respectively, staining (intensity and profile) was analyzed in sections of murine jejunum, stomach and epididymis as a function of glycocluster presence.
26553286	8	35	theme	generation	1327:1336	arg1	sensitivity					1305:1315	The marked and differential sensitivity	1277:1315	The marked and differential sensitivity of signal generation to structural aspects of the glycoclusters	1277:1379	The marked and differential sensitivity of signal generation to structural aspects of the glycoclusters proves the applicability of this method.
26553286	0	36	theme	tissue	128:133	arg1	context					135:141	the natural tissue context	116:141	the natural tissue context	116:141	Merging carbohydrate chemistry with lectin histochemistry to study inhibition of lectin binding by glycoclusters in the natural tissue context.
26553286	11	37	theme	tissue	1854:1859	arg1	lectin					1861:1866	a tissue lectin	1852:1866	a tissue lectin	1852:1866	Testing glycoclusters in lectin histochemistry will especially be attractive in cases of multi-target recognition (glycans, proteins and lipids) by a tissue lectin.
26553286	5	38	dep	lectins	861:867	arg1	i.e.					870:873	two plant lectins, i.e., GSA-II and WGA,	851:890	i.e.	870:873	In order to approach the natural context, we here introduce lectin histochemistry on fixed tissue sections to determine the susceptibility of binding of two plant lectins, i.e., GSA-II and WGA, to a series of 10 glycoclusters.
26553286	7	39	theme	presence	1267:1274	arg1	staining					1137:1144	staining	1137:1144	staining (intensity and profile)	1137:1168	Flanked by cell and solid-phase assays with human tumor lines and two mucins, respectively, staining (intensity and profile) was analyzed in sections of murine jejunum, stomach and epididymis as a function of glycocluster presence.
26553286	7	39	theme	presence	1267:1274	arg1	function					1242:1249	a function	1240:1249	a function of glycocluster presence	1240:1274	Flanked by cell and solid-phase assays with human tumor lines and two mucins, respectively, staining (intensity and profile) was analyzed in sections of murine jejunum, stomach and epididymis as a function of glycocluster presence.
26553286	10	40	theme	processing	1573:1582	arg1	sections					1591:1598	processing tissue sections	1573:1598	processing tissue sections	1573:1598	The special advantage of processing tissue sections is the monitoring of interference with lectin association at sites that are relevant for functionality.
26553286	7	41	with	assays	1077:1082	arg1	lines					1101:1105	human tumor lines	1089:1105	human tumor lines	1089:1105	Flanked by cell and solid-phase assays with human tumor lines and two mucins, respectively, staining (intensity and profile) was analyzed in sections of murine jejunum, stomach and epididymis as a function of glycocluster presence.
26553286	7	41	with	assays	1077:1082	arg1	mucins					1115:1120	two mucins	1111:1120	two mucins	1111:1120	Flanked by cell and solid-phase assays with human tumor lines and two mucins, respectively, staining (intensity and profile) was analyzed in sections of murine jejunum, stomach and epididymis as a function of glycocluster presence.
26553286	0	42	theme	natural	120:126	arg1	context					135:141	the natural tissue context	116:141	the natural tissue context	116:141	Merging carbohydrate chemistry with lectin histochemistry to study inhibition of lectin binding by glycoclusters in the natural tissue context.
26553286	8	43	theme	structural	1341:1350	arg1	aspects					1352:1358	structural aspects	1341:1358	structural aspects of the glycoclusters	1341:1379	The marked and differential sensitivity of signal generation to structural aspects of the glycoclusters proves the applicability of this method.
26553286	11	44	dep	recognition	1806:1816	arg1	lipids					1841:1846	lipids	1841:1846	lipids	1841:1846	Testing glycoclusters in lectin histochemistry will especially be attractive in cases of multi-target recognition (glycans, proteins and lipids) by a tissue lectin.
26553286	11	44	dep	recognition	1806:1816	arg1	proteins					1828:1835	proteins	1828:1835	proteins	1828:1835	Testing glycoclusters in lectin histochemistry will especially be attractive in cases of multi-target recognition (glycans, proteins and lipids) by a tissue lectin.
26553286	11	44	dep	recognition	1806:1816	arg1	glycans					1819:1825	glycans	1819:1825	glycans	1819:1825	Testing glycoclusters in lectin histochemistry will especially be attractive in cases of multi-target recognition (glycans, proteins and lipids) by a tissue lectin.
26553286	10	45	theme	at	1658:1659	arg1	sites					1661:1665	lectin association at sites	1639:1665	lectin association at sites that are relevant for functionality	1639:1701	The special advantage of processing tissue sections is the monitoring of interference with lectin association at sites that are relevant for functionality.
26553286	5	46	theme	lectins	861:867	arg1	binding					840:846	binding	840:846	binding of two plant lectins, i.e., GSA-II and WGA,	840:890	In order to approach the natural context, we here introduce lectin histochemistry on fixed tissue sections to determine the susceptibility of binding of two plant lectins, i.e., GSA-II and WGA, to a series of 10 glycoclusters.
26553286	3	47	theme	glycoconjugates	390:404	arg1	synthesis					372:380	The synthesis	368:380	The synthesis of (neo)glycoconjugates with bi- to oligo-valency (glycoclusters)	368:446	The synthesis of (neo)glycoconjugates with bi- to oligo-valency (glycoclusters) affords tools to delineate structure-activity relationships by blocking lectin binding to an artificial matrix, often a glycoprotein, or cultured cell lines.
26553286	7	48	theme	human	1089:1093	arg1	lines					1101:1105	human tumor lines	1089:1105	human tumor lines	1089:1105	Flanked by cell and solid-phase assays with human tumor lines and two mucins, respectively, staining (intensity and profile) was analyzed in sections of murine jejunum, stomach and epididymis as a function of glycocluster presence.
26553286	3	49	theme	cultured	585:592	arg1	lines					599:603	cultured cell lines	585:603	cultured cell lines	585:603	The synthesis of (neo)glycoconjugates with bi- to oligo-valency (glycoclusters) affords tools to delineate structure-activity relationships by blocking lectin binding to an artificial matrix, often a glycoprotein, or cultured cell lines.
26553286	5	50	theme	plant	855:859	arg1	lectins					861:867	two plant lectins	851:867	two plant lectins	851:867	In order to approach the natural context, we here introduce lectin histochemistry on fixed tissue sections to determine the susceptibility of binding of two plant lectins, i.e., GSA-II and WGA, to a series of 10 glycoclusters.
26553286	5	50	theme	plant	855:859	arg1	WGA					887:889	WGA	887:889	WGA	887:889	In order to approach the natural context, we here introduce lectin histochemistry on fixed tissue sections to determine the susceptibility of binding of two plant lectins, i.e., GSA-II and WGA, to a series of 10 glycoclusters.
26553286	5	50	theme	plant	855:859	arg1	GSA-II					876:881	GSA-II	876:881	GSA-II	876:881	In order to approach the natural context, we here introduce lectin histochemistry on fixed tissue sections to determine the susceptibility of binding of two plant lectins, i.e., GSA-II and WGA, to a series of 10 glycoclusters.
26553286	1	51	theme	growth	205:210	arg1	regulation					212:221	growth regulation	205:221	growth regulation	205:221	Recognition of glycans by lectins leads to cell adhesion and growth regulation.
26553286	0	52	theme	lectin	36:41	arg1	histochemistry					43:56	lectin histochemistry	36:56	lectin histochemistry	36:56	Merging carbohydrate chemistry with lectin histochemistry to study inhibition of lectin binding by glycoclusters in the natural tissue context.
26553286	5	53	theme	lectin	758:763	arg1	histochemistry					765:778	lectin histochemistry	758:778	lectin histochemistry on fixed tissue sections	758:803	In order to approach the natural context, we here introduce lectin histochemistry on fixed tissue sections to determine the susceptibility of binding of two plant lectins, i.e., GSA-II and WGA, to a series of 10 glycoclusters.
26553286	3	54	theme	cell	594:597	arg1	lines					599:603	cultured cell lines	585:603	cultured cell lines	585:603	The synthesis of (neo)glycoconjugates with bi- to oligo-valency (glycoclusters) affords tools to delineate structure-activity relationships by blocking lectin binding to an artificial matrix, often a glycoprotein, or cultured cell lines.
26553286	9	55	theme	tissue	1519:1524	arg1	glycoconjugates					1488:1502	glycoconjugates	1488:1502	glycoconjugates	1488:1502	This enables comparisons between data sets obtained by using (neo)glycoconjugates, cells and the tissue context as platforms.
26553286	9	55	theme	tissue	1519:1524	arg1	cells					1505:1509	cells	1505:1509	cells	1505:1509	This enables comparisons between data sets obtained by using (neo)glycoconjugates, cells and the tissue context as platforms.
26553286	9	55	theme	tissue	1519:1524	arg1	platforms					1537:1545	platforms	1537:1545	platforms	1537:1545	This enables comparisons between data sets obtained by using (neo)glycoconjugates, cells and the tissue context as platforms.
26553286	9	55	theme	tissue	1519:1524	arg1	context					1526:1532	the tissue context	1515:1532	the tissue context	1515:1532	This enables comparisons between data sets obtained by using (neo)glycoconjugates, cells and the tissue context as platforms.
26553286	2	56	dep	specificity	228:238	arg1	The					224:226	The	224:226	The	224:226	The specificity and selectivity of this process are determined by carbohydrate structure (sequence and shape) and topology of its presentation.
26553286	3	57	theme	neo	386:388	arg1	glycoconjugates					390:404	(neo)glycoconjugates	385:404	(neo)glycoconjugates	385:404	The synthesis of (neo)glycoconjugates with bi- to oligo-valency (glycoclusters) affords tools to delineate structure-activity relationships by blocking lectin binding to an artificial matrix, often a glycoprotein, or cultured cell lines.
26553286	6	58	theme	anomeric	975:982	arg1	α/β					994:996	α/β	994:996	α/β	994:996	Besides valency, this panel covers changes in the anomeric position (α/β) and the atom at the glycosidic linkage (O/S).
26553286	6	58	theme	anomeric	975:982	arg1	position					984:991	the anomeric position	971:991	the anomeric position (α/β)	971:997	Besides valency, this panel covers changes in the anomeric position (α/β) and the atom at the glycosidic linkage (O/S).
26553286	11	59	theme	multi-target	1793:1804	arg1	recognition					1806:1816	multi-target recognition	1793:1816	multi-target recognition (glycans, proteins and lipids) by a tissue lectin	1793:1866	Testing glycoclusters in lectin histochemistry will especially be attractive in cases of multi-target recognition (glycans, proteins and lipids) by a tissue lectin.
26553286	8	60	theme	differential	1292:1303	arg1	sensitivity					1305:1315	The marked and differential sensitivity	1277:1315	The marked and differential sensitivity of signal generation to structural aspects of the glycoclusters	1277:1379	The marked and differential sensitivity of signal generation to structural aspects of the glycoclusters proves the applicability of this method.
26553286	0	61	with	chemistry	21:29	arg1	histochemistry					43:56	lectin histochemistry	36:56	lectin histochemistry	36:56	Merging carbohydrate chemistry with lectin histochemistry to study inhibition of lectin binding by glycoclusters in the natural tissue context.
26553286	3	62	gly	glycoprotein	568:579	arg1	glycoprotein					568:579	often a glycoprotein	560:579	often a glycoprotein	560:579	The synthesis of (neo)glycoconjugates with bi- to oligo-valency (glycoclusters) affords tools to delineate structure-activity relationships by blocking lectin binding to an artificial matrix, often a glycoprotein, or cultured cell lines.
26553286	10	63	theme	interference	1621:1632	arg1	advantage					1560:1568	The special advantage	1548:1568	The special advantage of processing tissue sections	1548:1598	The special advantage of processing tissue sections is the monitoring of interference with lectin association at sites that are relevant for functionality.
26553286	10	63	theme	interference	1621:1632	arg1	monitoring					1607:1616	the monitoring	1603:1616	the monitoring of interference with lectin association at sites that are relevant for functionality	1603:1701	The special advantage of processing tissue sections is the monitoring of interference with lectin association at sites that are relevant for functionality.
26553286	11	64	theme	recognition	1806:1816	arg1	cases					1784:1788	cases	1784:1788	cases of multi-target recognition (glycans, proteins and lipids) by a tissue lectin	1784:1866	Testing glycoclusters in lectin histochemistry will especially be attractive in cases of multi-target recognition (glycans, proteins and lipids) by a tissue lectin.
26553286	8	65	theme	glycoclusters	1367:1379	arg1	aspects					1352:1358	structural aspects	1341:1358	structural aspects of the glycoclusters	1341:1379	The marked and differential sensitivity of signal generation to structural aspects of the glycoclusters proves the applicability of this method.
26553286	7	66	theme	glycocluster	1254:1265	arg1	presence					1267:1274	glycocluster presence	1254:1274	glycocluster presence	1254:1274	Flanked by cell and solid-phase assays with human tumor lines and two mucins, respectively, staining (intensity and profile) was analyzed in sections of murine jejunum, stomach and epididymis as a function of glycocluster presence.
26553286	2	67	dep	structure	303:311	arg1	shape					327:331	shape	327:331	shape	327:331	The specificity and selectivity of this process are determined by carbohydrate structure (sequence and shape) and topology of its presentation.
26553286	2	67	dep	structure	303:311	arg1	sequence					314:321	sequence	314:321	sequence	314:321	The specificity and selectivity of this process are determined by carbohydrate structure (sequence and shape) and topology of its presentation.
26553286	11	68	theme	lectin	1729:1734	arg1	histochemistry					1736:1749	lectin histochemistry	1729:1749	lectin histochemistry	1729:1749	Testing glycoclusters in lectin histochemistry will especially be attractive in cases of multi-target recognition (glycans, proteins and lipids) by a tissue lectin.
26553286	2	69	theme	process	264:270	arg1	specificity					228:238	specificity	228:238	specificity	228:238	The specificity and selectivity of this process are determined by carbohydrate structure (sequence and shape) and topology of its presentation.
26553286	2	69	theme	process	264:270	arg1	selectivity					244:254	selectivity	244:254	selectivity	244:254	The specificity and selectivity of this process are determined by carbohydrate structure (sequence and shape) and topology of its presentation.
26553286	10	70	theme	sections	1591:1598	arg1	advantage					1560:1568	The special advantage	1548:1568	The special advantage of processing tissue sections	1548:1598	The special advantage of processing tissue sections is the monitoring of interference with lectin association at sites that are relevant for functionality.
26553286	10	70	theme	sections	1591:1598	arg1	monitoring					1607:1616	the monitoring	1603:1616	the monitoring of interference with lectin association at sites that are relevant for functionality	1603:1701	The special advantage of processing tissue sections is the monitoring of interference with lectin association at sites that are relevant for functionality.
26553286	10	71	theme	lectin	1639:1644	arg1	sites					1661:1665	lectin association at sites	1639:1665	lectin association at sites that are relevant for functionality	1639:1701	The special advantage of processing tissue sections is the monitoring of interference with lectin association at sites that are relevant for functionality.
26553286	0	72	from	glycoclusters	99:111	arg1	context					135:141	the natural tissue context	116:141	the natural tissue context	116:141	Merging carbohydrate chemistry with lectin histochemistry to study inhibition of lectin binding by glycoclusters in the natural tissue context.
26553286	3	73	theme	artificial	541:550	arg1	matrix					552:557	an artificial matrix	538:557	an artificial matrix	538:557	The synthesis of (neo)glycoconjugates with bi- to oligo-valency (glycoclusters) affords tools to delineate structure-activity relationships by blocking lectin binding to an artificial matrix, often a glycoprotein, or cultured cell lines.
26553286	10	74	theme	special	1552:1558	arg1	advantage					1560:1568	The special advantage	1548:1568	The special advantage of processing tissue sections	1548:1598	The special advantage of processing tissue sections is the monitoring of interference with lectin association at sites that are relevant for functionality.
26553286	10	74	theme	special	1552:1558	arg1	monitoring					1607:1616	the monitoring	1603:1616	the monitoring of interference with lectin association at sites that are relevant for functionality	1603:1701	The special advantage of processing tissue sections is the monitoring of interference with lectin association at sites that are relevant for functionality.
26553286	6	75	theme	glycosidic	1019:1028	arg1	O/S					1039:1041	O/S	1039:1041	O/S	1039:1041	Besides valency, this panel covers changes in the anomeric position (α/β) and the atom at the glycosidic linkage (O/S).
26553286	6	75	theme	glycosidic	1019:1028	arg1	linkage					1030:1036	the glycosidic linkage	1015:1036	the glycosidic linkage (O/S)	1015:1042	Besides valency, this panel covers changes in the anomeric position (α/β) and the atom at the glycosidic linkage (O/S).
26553286	10	76	from	sites	1661:1665	arg1	at					1658:1659	lectin association at sites	1639:1665	lectin association at sites that are relevant for functionality	1639:1701	The special advantage of processing tissue sections is the monitoring of interference with lectin association at sites that are relevant for functionality.
26553286	10	76	from	sites	1661:1665	arg1	lectin					1639:1644	lectin association at sites	1639:1665	lectin association at sites that are relevant for functionality	1639:1701	The special advantage of processing tissue sections is the monitoring of interference with lectin association at sites that are relevant for functionality.
26553286	10	76	from	sites	1661:1665	arg1	association					1646:1656	lectin association at sites	1639:1665	lectin association at sites that are relevant for functionality	1639:1701	The special advantage of processing tissue sections is the monitoring of interference with lectin association at sites that are relevant for functionality.
26553286	4	77	theme	assays	628:633	arg1	drawback					610:617	The drawback	606:617	The drawback of these assays	606:633	The drawback of these assays is that glycan presentation is different from that in tissues.
26553286	7	78	theme	epididymis	1226:1235	arg1	sections					1186:1193	sections	1186:1193	sections of murine jejunum, stomach and epididymis	1186:1235	Flanked by cell and solid-phase assays with human tumor lines and two mucins, respectively, staining (intensity and profile) was analyzed in sections of murine jejunum, stomach and epididymis as a function of glycocluster presence.
26553286	3	79	theme	lectin	520:525	arg1	binding					527:533	lectin binding	520:533	lectin binding to an artificial matrix, often a glycoprotein, or cultured cell lines	520:603	The synthesis of (neo)glycoconjugates with bi- to oligo-valency (glycoclusters) affords tools to delineate structure-activity relationships by blocking lectin binding to an artificial matrix, often a glycoprotein, or cultured cell lines.
26553286	5	80	from	histochemistry	765:778	arg1	sections					796:803	fixed tissue sections	783:803	fixed tissue sections	783:803	In order to approach the natural context, we here introduce lectin histochemistry on fixed tissue sections to determine the susceptibility of binding of two plant lectins, i.e., GSA-II and WGA, to a series of 10 glycoclusters.
26553286	8	81	theme	signal	1320:1325	arg1	generation					1327:1336	signal generation	1320:1336	signal generation	1320:1336	The marked and differential sensitivity of signal generation to structural aspects of the glycoclusters proves the applicability of this method.
26553286	6	82	from	linkage	1030:1036	arg1	changes					960:966	changes	960:966	changes in the anomeric position (α/β)	960:997	Besides valency, this panel covers changes in the anomeric position (α/β) and the atom at the glycosidic linkage (O/S).
26553286	6	82	from	linkage	1030:1036	arg1	atom					1007:1010	the atom	1003:1010	the atom at the glycosidic linkage (O/S)	1003:1042	Besides valency, this panel covers changes in the anomeric position (α/β) and the atom at the glycosidic linkage (O/S).
26553286	2	83	theme	presentation	354:365	arg1	topology					338:345	topology	338:345	topology of its presentation	338:365	The specificity and selectivity of this process are determined by carbohydrate structure (sequence and shape) and topology of its presentation.
26553286	2	83	theme	presentation	354:365	arg1	structure					303:311	carbohydrate structure	290:311	carbohydrate structure (sequence and shape)	290:332	The specificity and selectivity of this process are determined by carbohydrate structure (sequence and shape) and topology of its presentation.
26484870	6	0	theme	gB	1114:1115	arg1	plasticity					1100:1109	structural plasticity	1089:1109	structural plasticity of gB that may accommodate virus-specific functional requirements	1089:1175	Despite structural similarities, each gB has a unique domain arrangement, demonstrating structural plasticity of gB that may accommodate virus-specific functional requirements.
26484870	10	1	theme	immunogenic	1777:1787	arg1	properties					1789:1798	its antigenic and immunogenic properties	1759:1798	its antigenic and immunogenic properties	1759:1798	HCMV gB structure provides a starting point for elucidation of its antigenic and immunogenic properties and aid in the design of recombinant vaccines and monoclonal antibody therapies.
26484870	10	2	theme	antigenic	1763:1771	arg1	properties					1789:1798	its antigenic and immunogenic properties	1759:1798	its antigenic and immunogenic properties	1759:1798	HCMV gB structure provides a starting point for elucidation of its antigenic and immunogenic properties and aid in the design of recombinant vaccines and monoclonal antibody therapies.
26484870	4	3	theme	first	782:786	arg1	structure					801:809	the first atomic-level structure	778:809	the first atomic-level structure of any betaherpesvirus glycoprotein	778:845	Here, we report the crystal structure of the HCMV gB ectodomain determined to 3.6-Å resolution, which is the first atomic-level structure of any betaherpesvirus glycoprotein.
26484870	4	3	theme	first	782:786	arg1	structure					701:709	the crystal structure	689:709	the crystal structure	689:709	Here, we report the crystal structure of the HCMV gB ectodomain determined to 3.6-Å resolution, which is the first atomic-level structure of any betaherpesvirus glycoprotein.
26484870	5	4	theme	viral	985:989	arg1	fusogens					991:998	the class III viral fusogens	971:998	the class III viral fusogens	971:998	The structure of HCMV gB resembles the postfusion structures of HSV-1 and EBV homologs, establishing it as a new member of the class III viral fusogens.
26484870	6	5	contain	has	1042:1044	arg2	arrangement					1062:1072	a unique domain arrangement	1046:1072	a unique domain arrangement	1046:1072	Despite structural similarities, each gB has a unique domain arrangement, demonstrating structural plasticity of gB that may accommodate virus-specific functional requirements.
26484870	6	5	contain	has	1042:1044	arg1	gB					1039:1040	each gB	1034:1040	each gB	1034:1040	Despite structural similarities, each gB has a unique domain arrangement, demonstrating structural plasticity of gB that may accommodate virus-specific functional requirements.
26484870	10	6	theme	recombinant	1825:1835	arg1	vaccines					1837:1844	recombinant vaccines	1825:1844	recombinant vaccines	1825:1844	HCMV gB structure provides a starting point for elucidation of its antigenic and immunogenic properties and aid in the design of recombinant vaccines and monoclonal antibody therapies.
26484870	0	7	theme	organ	257:261	arg1	recipients					274:283	organ transplant recipients	257:283	organ transplant recipients	257:283	Crystal Structure of the Human Cytomegalovirus Glycoprotein B. Human cytomegalovirus (HCMV), a dsDNA, enveloped virus, is a ubiquitous pathogen that establishes lifelong latent infections and caused disease in persons with compromised immune systems, e.g., organ transplant recipients or AIDS patients.
26484870	4	8	theme	glycoprotein	834:845	arg1	structure					801:809	the first atomic-level structure	778:809	the first atomic-level structure of any betaherpesvirus glycoprotein	778:845	Here, we report the crystal structure of the HCMV gB ectodomain determined to 3.6-Å resolution, which is the first atomic-level structure of any betaherpesvirus glycoprotein.
26484870	4	8	theme	glycoprotein	834:845	arg1	structure					701:709	the crystal structure	689:709	the crystal structure	689:709	Here, we report the crystal structure of the HCMV gB ectodomain determined to 3.6-Å resolution, which is the first atomic-level structure of any betaherpesvirus glycoprotein.
26484870	2	9	theme	signaling	499:507	arg1	receptors					509:517	signaling receptors	499:517	signaling receptors	499:517	Entry of HCMV into cells requires the conserved glycoprotein B (gB), thought to function as a fusogen and reported to bind signaling receptors.
26484870	6	10	theme	virus-specific	1138:1151	arg1	requirements					1164:1175	virus-specific functional requirements	1138:1175	virus-specific functional requirements	1138:1175	Despite structural similarities, each gB has a unique domain arrangement, demonstrating structural plasticity of gB that may accommodate virus-specific functional requirements.
26484870	0	11	theme	ubiquitous	124:133	arg1	Structure					8:16	Crystal Structure	0:16	Crystal Structure of the Human Cytomegalovirus Glycoprotein B. Human cytomegalovirus (HCMV), a dsDNA, enveloped virus,	0:117	Crystal Structure of the Human Cytomegalovirus Glycoprotein B. Human cytomegalovirus (HCMV), a dsDNA, enveloped virus, is a ubiquitous pathogen that establishes lifelong latent infections and caused disease in persons with compromised immune systems, e.g., organ transplant recipients or AIDS patients.
26484870	0	11	theme	ubiquitous	124:133	arg1	pathogen					135:142	a ubiquitous pathogen	122:142	a ubiquitous pathogen that establishes lifelong latent infections and caused disease in persons with compromised immune systems, e.g., organ transplant recipients or AIDS patients	122:300	Crystal Structure of the Human Cytomegalovirus Glycoprotein B. Human cytomegalovirus (HCMV), a dsDNA, enveloped virus, is a ubiquitous pathogen that establishes lifelong latent infections and caused disease in persons with compromised immune systems, e.g., organ transplant recipients or AIDS patients.
26484870	5	12	theme	class	975:979	arg1	fusogens					991:998	the class III viral fusogens	971:998	the class III viral fusogens	971:998	The structure of HCMV gB resembles the postfusion structures of HSV-1 and EBV homologs, establishing it as a new member of the class III viral fusogens.
26484870	0	13	theme	AIDS	288:291	arg1	patients					293:300	AIDS patients	288:300	AIDS patients	288:300	Crystal Structure of the Human Cytomegalovirus Glycoprotein B. Human cytomegalovirus (HCMV), a dsDNA, enveloped virus, is a ubiquitous pathogen that establishes lifelong latent infections and caused disease in persons with compromised immune systems, e.g., organ transplant recipients or AIDS patients.
26484870	5	14	theme	gB	870:871	arg1	structure					852:860	The structure	848:860	The structure of HCMV gB	848:871	The structure of HCMV gB resembles the postfusion structures of HSV-1 and EBV homologs, establishing it as a new member of the class III viral fusogens.
26484870	8	15	theme	Antigenic	1286:1294	arg1	sites					1296:1300	Antigenic sites	1286:1300	Antigenic sites that elicit neutralizing antibodies	1286:1336	Antigenic sites that elicit neutralizing antibodies are more heavily glycosylated than those that elicit non-neutralizing antibodies, which suggest that HCMV gB uses glycans to shield neutralizing epitopes while exposing non-neutralizing epitopes.
26484870	0	16	theme	Human	63:67	arg1	virus					112:116	enveloped virus	102:116	enveloped virus	102:116	Crystal Structure of the Human Cytomegalovirus Glycoprotein B. Human cytomegalovirus (HCMV), a dsDNA, enveloped virus, is a ubiquitous pathogen that establishes lifelong latent infections and caused disease in persons with compromised immune systems, e.g., organ transplant recipients or AIDS patients.
26484870	0	16	theme	Human	63:67	arg1	HCMV					86:89	HCMV	86:89	HCMV	86:89	Crystal Structure of the Human Cytomegalovirus Glycoprotein B. Human cytomegalovirus (HCMV), a dsDNA, enveloped virus, is a ubiquitous pathogen that establishes lifelong latent infections and caused disease in persons with compromised immune systems, e.g., organ transplant recipients or AIDS patients.
26484870	0	16	theme	Human	63:67	arg1	cytomegalovirus					69:83	B. Human cytomegalovirus	60:83	B. Human cytomegalovirus (HCMV)	60:90	Crystal Structure of the Human Cytomegalovirus Glycoprotein B. Human cytomegalovirus (HCMV), a dsDNA, enveloped virus, is a ubiquitous pathogen that establishes lifelong latent infections and caused disease in persons with compromised immune systems, e.g., organ transplant recipients or AIDS patients.
26484870	0	16	theme	Human	63:67	arg1	dsDNA					95:99	a dsDNA	93:99	a dsDNA	93:99	Crystal Structure of the Human Cytomegalovirus Glycoprotein B. Human cytomegalovirus (HCMV), a dsDNA, enveloped virus, is a ubiquitous pathogen that establishes lifelong latent infections and caused disease in persons with compromised immune systems, e.g., organ transplant recipients or AIDS patients.
26484870	3	17	theme	immune	545:550	arg1	response					552:559	a strong immune response	536:559	a strong immune response	536:559	gB also elicits a strong immune response in humans and induces the production of neutralizing antibodies although most anti-gB Abs are non-neutralizing.
26484870	4	18	gly	glycoprotein	834:845	arg1	glycoprotein					834:845	any betaherpesvirus glycoprotein	814:845	any betaherpesvirus glycoprotein	814:845	Here, we report the crystal structure of the HCMV gB ectodomain determined to 3.6-Å resolution, which is the first atomic-level structure of any betaherpesvirus glycoprotein.
26484870	9	19	theme	glycosylation	1539:1551	arg1	pattern					1553:1559	This glycosylation pattern	1534:1559	This glycosylation pattern	1534:1559	This glycosylation pattern may have evolved to direct the immune response towards generation of non-neutralizing antibodies thus helping HCMV to avoid clearance.
26484870	8	20	theme	neutralizing	1314:1325	arg1	antibodies					1327:1336	neutralizing antibodies	1314:1336	neutralizing antibodies	1314:1336	Antigenic sites that elicit neutralizing antibodies are more heavily glycosylated than those that elicit non-neutralizing antibodies, which suggest that HCMV gB uses glycans to shield neutralizing epitopes while exposing non-neutralizing epitopes.
26484870	1	21	theme	leading	318:324	arg1	cause					326:330	a leading cause	316:330	a leading cause of congenital viral infections in newborns	316:373	HCMV is also a leading cause of congenital viral infections in newborns.
26484870	1	21	theme	leading	318:324	arg1	HCMV					303:306	HCMV	303:306	HCMV	303:306	HCMV is also a leading cause of congenital viral infections in newborns.
26484870	2	22	dep	B	437:437	arg1	reported					482:489	reported	482:489	reported to bind signaling receptors	482:517	Entry of HCMV into cells requires the conserved glycoprotein B (gB), thought to function as a fusogen and reported to bind signaling receptors.
26484870	2	22	dep	B	437:437	arg1	thought					445:451	thought	445:451	thought to function as a fusogen	445:476	Entry of HCMV into cells requires the conserved glycoprotein B (gB), thought to function as a fusogen and reported to bind signaling receptors.
26484870	0	23	theme	compromised	223:233	arg1	systems					242:248	compromised immune systems	223:248	compromised immune systems	223:248	Crystal Structure of the Human Cytomegalovirus Glycoprotein B. Human cytomegalovirus (HCMV), a dsDNA, enveloped virus, is a ubiquitous pathogen that establishes lifelong latent infections and caused disease in persons with compromised immune systems, e.g., organ transplant recipients or AIDS patients.
26484870	5	24	theme	HSV-1	912:916	arg1	homologs					926:933	HSV-1 and EBV homologs	912:933	HSV-1 and EBV homologs	912:933	The structure of HCMV gB resembles the postfusion structures of HSV-1 and EBV homologs, establishing it as a new member of the class III viral fusogens.
26484870	5	25	theme	EBV	922:924	arg1	homologs					926:933	HSV-1 and EBV homologs	912:933	HSV-1 and EBV homologs	912:933	The structure of HCMV gB resembles the postfusion structures of HSV-1 and EBV homologs, establishing it as a new member of the class III viral fusogens.
26484870	0	26	theme	Crystal	0:6	arg1	Structure					8:16	Crystal Structure	0:16	Crystal Structure of the Human Cytomegalovirus Glycoprotein B. Human cytomegalovirus (HCMV), a dsDNA, enveloped virus,	0:117	Crystal Structure of the Human Cytomegalovirus Glycoprotein B. Human cytomegalovirus (HCMV), a dsDNA, enveloped virus, is a ubiquitous pathogen that establishes lifelong latent infections and caused disease in persons with compromised immune systems, e.g., organ transplant recipients or AIDS patients.
26484870	0	26	theme	Crystal	0:6	arg1	pathogen					135:142	a ubiquitous pathogen	122:142	a ubiquitous pathogen that establishes lifelong latent infections and caused disease in persons with compromised immune systems, e.g., organ transplant recipients or AIDS patients	122:300	Crystal Structure of the Human Cytomegalovirus Glycoprotein B. Human cytomegalovirus (HCMV), a dsDNA, enveloped virus, is a ubiquitous pathogen that establishes lifelong latent infections and caused disease in persons with compromised immune systems, e.g., organ transplant recipients or AIDS patients.
26484870	3	27	theme	strong	538:543	arg1	response					552:559	a strong immune response	536:559	a strong immune response	536:559	gB also elicits a strong immune response in humans and induces the production of neutralizing antibodies although most anti-gB Abs are non-neutralizing.
26484870	2	28	theme	HCMV	385:388	arg1	Entry					376:380	Entry	376:380	Entry of HCMV into cells	376:399	Entry of HCMV into cells requires the conserved glycoprotein B (gB), thought to function as a fusogen and reported to bind signaling receptors.
26484870	1	29	from	cause	326:330	arg1	newborns					366:373	newborns	366:373	newborns	366:373	HCMV is also a leading cause of congenital viral infections in newborns.
26484870	9	30	theme	immune	1592:1597	arg1	response					1599:1606	the immune response	1588:1606	the immune response towards generation of non-neutralizing antibodies	1588:1656	This glycosylation pattern may have evolved to direct the immune response towards generation of non-neutralizing antibodies thus helping HCMV to avoid clearance.
26484870	4	31	theme	3.6-Å	751:755	arg1	resolution					757:766	3.6-Å resolution	751:766	3.6-Å resolution	751:766	Here, we report the crystal structure of the HCMV gB ectodomain determined to 3.6-Å resolution, which is the first atomic-level structure of any betaherpesvirus glycoprotein.
26484870	10	32	theme	HCMV	1696:1699	arg1	structure					1704:1712	HCMV gB structure	1696:1712	HCMV gB structure	1696:1712	HCMV gB structure provides a starting point for elucidation of its antigenic and immunogenic properties and aid in the design of recombinant vaccines and monoclonal antibody therapies.
26484870	0	33	theme	Human	25:29	arg1	Glycoprotein					47:58	the Human Cytomegalovirus Glycoprotein B. Human cytomegalovirus (HCMV), a dsDNA, enveloped virus,	21:117	the Human Cytomegalovirus Glycoprotein B. Human cytomegalovirus (HCMV), a dsDNA, enveloped virus,	21:117	Crystal Structure of the Human Cytomegalovirus Glycoprotein B. Human cytomegalovirus (HCMV), a dsDNA, enveloped virus, is a ubiquitous pathogen that establishes lifelong latent infections and caused disease in persons with compromised immune systems, e.g., organ transplant recipients or AIDS patients.
26484870	0	34	dep	recipients	274:283	arg1	e.g.					251:254	e.g.	251:254	e.g.	251:254	Crystal Structure of the Human Cytomegalovirus Glycoprotein B. Human cytomegalovirus (HCMV), a dsDNA, enveloped virus, is a ubiquitous pathogen that establishes lifelong latent infections and caused disease in persons with compromised immune systems, e.g., organ transplant recipients or AIDS patients.
26484870	0	35	dep	Glycoprotein	47:58	arg1	virus					112:116	enveloped virus	102:116	enveloped virus	102:116	Crystal Structure of the Human Cytomegalovirus Glycoprotein B. Human cytomegalovirus (HCMV), a dsDNA, enveloped virus, is a ubiquitous pathogen that establishes lifelong latent infections and caused disease in persons with compromised immune systems, e.g., organ transplant recipients or AIDS patients.
26484870	0	35	dep	Glycoprotein	47:58	arg1	HCMV					86:89	HCMV	86:89	HCMV	86:89	Crystal Structure of the Human Cytomegalovirus Glycoprotein B. Human cytomegalovirus (HCMV), a dsDNA, enveloped virus, is a ubiquitous pathogen that establishes lifelong latent infections and caused disease in persons with compromised immune systems, e.g., organ transplant recipients or AIDS patients.
26484870	0	35	dep	Glycoprotein	47:58	arg1	cytomegalovirus					69:83	B. Human cytomegalovirus	60:83	B. Human cytomegalovirus (HCMV)	60:90	Crystal Structure of the Human Cytomegalovirus Glycoprotein B. Human cytomegalovirus (HCMV), a dsDNA, enveloped virus, is a ubiquitous pathogen that establishes lifelong latent infections and caused disease in persons with compromised immune systems, e.g., organ transplant recipients or AIDS patients.
26484870	0	35	dep	Glycoprotein	47:58	arg1	dsDNA					95:99	a dsDNA	93:99	a dsDNA	93:99	Crystal Structure of the Human Cytomegalovirus Glycoprotein B. Human cytomegalovirus (HCMV), a dsDNA, enveloped virus, is a ubiquitous pathogen that establishes lifelong latent infections and caused disease in persons with compromised immune systems, e.g., organ transplant recipients or AIDS patients.
26484870	2	36	theme	glycoprotein	424:435	arg1	gB					440:441	gB	440:441	gB	440:441	Entry of HCMV into cells requires the conserved glycoprotein B (gB), thought to function as a fusogen and reported to bind signaling receptors.
26484870	2	36	theme	glycoprotein	424:435	arg1	B					437:437	glycoprotein B	424:437	the conserved glycoprotein B (gB)	410:442	Entry of HCMV into cells requires the conserved glycoprotein B (gB), thought to function as a fusogen and reported to bind signaling receptors.
26484870	8	37	theme	non-neutralizing	1391:1406	arg1	antibodies					1408:1417	non-neutralizing antibodies	1391:1417	non-neutralizing antibodies	1391:1417	Antigenic sites that elicit neutralizing antibodies are more heavily glycosylated than those that elicit non-neutralizing antibodies, which suggest that HCMV gB uses glycans to shield neutralizing epitopes while exposing non-neutralizing epitopes.
26484870	0	38	theme	Glycoprotein	47:58	arg1	Structure					8:16	Crystal Structure	0:16	Crystal Structure of the Human Cytomegalovirus Glycoprotein B. Human cytomegalovirus (HCMV), a dsDNA, enveloped virus,	0:117	Crystal Structure of the Human Cytomegalovirus Glycoprotein B. Human cytomegalovirus (HCMV), a dsDNA, enveloped virus, is a ubiquitous pathogen that establishes lifelong latent infections and caused disease in persons with compromised immune systems, e.g., organ transplant recipients or AIDS patients.
26484870	0	38	theme	Glycoprotein	47:58	arg1	pathogen					135:142	a ubiquitous pathogen	122:142	a ubiquitous pathogen that establishes lifelong latent infections and caused disease in persons with compromised immune systems, e.g., organ transplant recipients or AIDS patients	122:300	Crystal Structure of the Human Cytomegalovirus Glycoprotein B. Human cytomegalovirus (HCMV), a dsDNA, enveloped virus, is a ubiquitous pathogen that establishes lifelong latent infections and caused disease in persons with compromised immune systems, e.g., organ transplant recipients or AIDS patients.
26484870	8	39	gly	glycosylated	1355:1366	arg1	sites					1296:1300	Antigenic sites	1286:1300	Antigenic sites that elicit neutralizing antibodies	1286:1336	Antigenic sites that elicit neutralizing antibodies are more heavily glycosylated than those that elicit non-neutralizing antibodies, which suggest that HCMV gB uses glycans to shield neutralizing epitopes while exposing non-neutralizing epitopes.
26484870	2	40	gly	glycoprotein	424:435	arg1	glycoprotein					424:435	glycoprotein B	424:437	the conserved glycoprotein B (gB)	410:442	Entry of HCMV into cells requires the conserved glycoprotein B (gB), thought to function as a fusogen and reported to bind signaling receptors.
26484870	7	41	theme	gB	1239:1240	arg1	ectodomain					1242:1251	the gB ectodomain	1235:1251	the gB ectodomain	1235:1251	The structure illustrates how extensive glycosylation of the gB ectodomain influences antibody recognition.
26484870	3	42	theme	antibodies	614:623	arg1	production					587:596	the production	583:596	the production of neutralizing antibodies	583:623	gB also elicits a strong immune response in humans and induces the production of neutralizing antibodies although most anti-gB Abs are non-neutralizing.
26484870	4	43	theme	gB	723:724	arg1	ectodomain					726:735	the HCMV gB ectodomain	714:735	the HCMV gB ectodomain determined to 3.6-Å resolution	714:766	Here, we report the crystal structure of the HCMV gB ectodomain determined to 3.6-Å resolution, which is the first atomic-level structure of any betaherpesvirus glycoprotein.
26484870	7	44	gly	glycosylation	1218:1230	arg1	ectodomain					1242:1251	the gB ectodomain	1235:1251	the gB ectodomain	1235:1251	The structure illustrates how extensive glycosylation of the gB ectodomain influences antibody recognition.
26484870	9	45	theme	antibodies	1647:1656	arg1	generation					1616:1625	generation	1616:1625	generation of non-neutralizing antibodies	1616:1656	This glycosylation pattern may have evolved to direct the immune response towards generation of non-neutralizing antibodies thus helping HCMV to avoid clearance.
26484870	7	46	theme	extensive	1208:1216	arg1	glycosylation					1218:1230	extensive glycosylation	1208:1230	extensive glycosylation of the gB ectodomain	1208:1251	The structure illustrates how extensive glycosylation of the gB ectodomain influences antibody recognition.
26484870	0	47	with	persons	210:216	arg1	systems					242:248	compromised immune systems	223:248	compromised immune systems	223:248	Crystal Structure of the Human Cytomegalovirus Glycoprotein B. Human cytomegalovirus (HCMV), a dsDNA, enveloped virus, is a ubiquitous pathogen that establishes lifelong latent infections and caused disease in persons with compromised immune systems, e.g., organ transplant recipients or AIDS patients.
26484870	4	48	theme	atomic-level	788:799	arg1	structure					801:809	the first atomic-level structure	778:809	the first atomic-level structure of any betaherpesvirus glycoprotein	778:845	Here, we report the crystal structure of the HCMV gB ectodomain determined to 3.6-Å resolution, which is the first atomic-level structure of any betaherpesvirus glycoprotein.
26484870	4	48	theme	atomic-level	788:799	arg1	structure					701:709	the crystal structure	689:709	the crystal structure	689:709	Here, we report the crystal structure of the HCMV gB ectodomain determined to 3.6-Å resolution, which is the first atomic-level structure of any betaherpesvirus glycoprotein.
26484870	6	49	theme	structural	1009:1018	arg1	similarities					1020:1031	structural similarities	1009:1031	structural similarities	1009:1031	Despite structural similarities, each gB has a unique domain arrangement, demonstrating structural plasticity of gB that may accommodate virus-specific functional requirements.
26484870	3	50	theme	most	634:637	arg1	Abs					647:649	most anti-gB Abs	634:649	most anti-gB Abs	634:649	gB also elicits a strong immune response in humans and induces the production of neutralizing antibodies although most anti-gB Abs are non-neutralizing.
26484870	0	51	theme	latent	170:175	arg1	infections					177:186	lifelong latent infections	161:186	lifelong latent infections	161:186	Crystal Structure of the Human Cytomegalovirus Glycoprotein B. Human cytomegalovirus (HCMV), a dsDNA, enveloped virus, is a ubiquitous pathogen that establishes lifelong latent infections and caused disease in persons with compromised immune systems, e.g., organ transplant recipients or AIDS patients.
26484870	10	52	theme	properties	1789:1798	arg1	elucidation					1744:1754	elucidation	1744:1754	elucidation of its antigenic and immunogenic properties	1744:1798	HCMV gB structure provides a starting point for elucidation of its antigenic and immunogenic properties and aid in the design of recombinant vaccines and monoclonal antibody therapies.
26484870	6	53	theme	structural	1089:1098	arg1	plasticity					1100:1109	structural plasticity	1089:1109	structural plasticity of gB that may accommodate virus-specific functional requirements	1089:1175	Despite structural similarities, each gB has a unique domain arrangement, demonstrating structural plasticity of gB that may accommodate virus-specific functional requirements.
26484870	0	54	theme	enveloped	102:110	arg1	virus					112:116	enveloped virus	102:116	enveloped virus	102:116	Crystal Structure of the Human Cytomegalovirus Glycoprotein B. Human cytomegalovirus (HCMV), a dsDNA, enveloped virus, is a ubiquitous pathogen that establishes lifelong latent infections and caused disease in persons with compromised immune systems, e.g., organ transplant recipients or AIDS patients.
26484870	0	54	theme	enveloped	102:110	arg1	cytomegalovirus					69:83	B. Human cytomegalovirus	60:83	B. Human cytomegalovirus (HCMV)	60:90	Crystal Structure of the Human Cytomegalovirus Glycoprotein B. Human cytomegalovirus (HCMV), a dsDNA, enveloped virus, is a ubiquitous pathogen that establishes lifelong latent infections and caused disease in persons with compromised immune systems, e.g., organ transplant recipients or AIDS patients.
26484870	8	55	theme	HCMV	1439:1442	arg1	gB					1444:1445	HCMV gB	1439:1445	HCMV gB	1439:1445	Antigenic sites that elicit neutralizing antibodies are more heavily glycosylated than those that elicit non-neutralizing antibodies, which suggest that HCMV gB uses glycans to shield neutralizing epitopes while exposing non-neutralizing epitopes.
26484870	6	56	theme	domain	1055:1060	arg1	arrangement					1062:1072	a unique domain arrangement	1046:1072	a unique domain arrangement	1046:1072	Despite structural similarities, each gB has a unique domain arrangement, demonstrating structural plasticity of gB that may accommodate virus-specific functional requirements.
26484870	10	57	dep	design	1815:1820	arg1	therapies					1870:1878	therapies	1870:1878	therapies	1870:1878	HCMV gB structure provides a starting point for elucidation of its antigenic and immunogenic properties and aid in the design of recombinant vaccines and monoclonal antibody therapies.
26484870	5	58	theme	fusogens	991:998	arg1	it					949:950	it	949:950	it	949:950	The structure of HCMV gB resembles the postfusion structures of HSV-1 and EBV homologs, establishing it as a new member of the class III viral fusogens.
26484870	5	58	theme	fusogens	991:998	arg1	member					961:966	a new member	955:966	a new member of the class III viral fusogens	955:998	The structure of HCMV gB resembles the postfusion structures of HSV-1 and EBV homologs, establishing it as a new member of the class III viral fusogens.
26484870	10	59	theme	vaccines	1837:1844	arg1	design					1815:1820	the design	1811:1820	the design of recombinant vaccines	1811:1844	HCMV gB structure provides a starting point for elucidation of its antigenic and immunogenic properties and aid in the design of recombinant vaccines and monoclonal antibody therapies.
26484870	10	59	theme	vaccines	1837:1844	arg1	antibody					1861:1868	monoclonal antibody	1850:1868	monoclonal antibody	1850:1868	HCMV gB structure provides a starting point for elucidation of its antigenic and immunogenic properties and aid in the design of recombinant vaccines and monoclonal antibody therapies.
26484870	4	60	theme	betaherpesvirus	818:832	arg1	glycoprotein					834:845	any betaherpesvirus glycoprotein	814:845	any betaherpesvirus glycoprotein	814:845	Here, we report the crystal structure of the HCMV gB ectodomain determined to 3.6-Å resolution, which is the first atomic-level structure of any betaherpesvirus glycoprotein.
26484870	6	61	theme	functional	1153:1162	arg1	requirements					1164:1175	virus-specific functional requirements	1138:1175	virus-specific functional requirements	1138:1175	Despite structural similarities, each gB has a unique domain arrangement, demonstrating structural plasticity of gB that may accommodate virus-specific functional requirements.
26484870	9	62	theme	non-neutralizing	1630:1645	arg1	antibodies					1647:1656	non-neutralizing antibodies	1630:1656	non-neutralizing antibodies	1630:1656	This glycosylation pattern may have evolved to direct the immune response towards generation of non-neutralizing antibodies thus helping HCMV to avoid clearance.
26484870	0	63	theme	transplant	263:272	arg1	recipients					274:283	organ transplant recipients	257:283	organ transplant recipients	257:283	Crystal Structure of the Human Cytomegalovirus Glycoprotein B. Human cytomegalovirus (HCMV), a dsDNA, enveloped virus, is a ubiquitous pathogen that establishes lifelong latent infections and caused disease in persons with compromised immune systems, e.g., organ transplant recipients or AIDS patients.
26484870	5	64	theme	HCMV	865:868	arg1	gB					870:871	HCMV gB	865:871	HCMV gB	865:871	The structure of HCMV gB resembles the postfusion structures of HSV-1 and EBV homologs, establishing it as a new member of the class III viral fusogens.
26484870	8	65	theme	neutralizing	1470:1481	arg1	epitopes					1483:1490	neutralizing epitopes	1470:1490	neutralizing epitopes	1470:1490	Antigenic sites that elicit neutralizing antibodies are more heavily glycosylated than those that elicit non-neutralizing antibodies, which suggest that HCMV gB uses glycans to shield neutralizing epitopes while exposing non-neutralizing epitopes.
26484870	10	66	theme	monoclonal	1850:1859	arg1	antibody					1861:1868	monoclonal antibody	1850:1868	monoclonal antibody	1850:1868	HCMV gB structure provides a starting point for elucidation of its antigenic and immunogenic properties and aid in the design of recombinant vaccines and monoclonal antibody therapies.
26484870	8	67	theme	non-neutralizing	1507:1522	arg1	epitopes					1524:1531	non-neutralizing epitopes	1507:1531	non-neutralizing epitopes	1507:1531	Antigenic sites that elicit neutralizing antibodies are more heavily glycosylated than those that elicit non-neutralizing antibodies, which suggest that HCMV gB uses glycans to shield neutralizing epitopes while exposing non-neutralizing epitopes.
26484870	1	68	theme	viral	346:350	arg1	infections					352:361	congenital viral infections	335:361	congenital viral infections	335:361	HCMV is also a leading cause of congenital viral infections in newborns.
26484870	4	69	theme	crystal	693:699	arg1	structure					701:709	the crystal structure	689:709	the crystal structure	689:709	Here, we report the crystal structure of the HCMV gB ectodomain determined to 3.6-Å resolution, which is the first atomic-level structure of any betaherpesvirus glycoprotein.
26484870	4	69	theme	crystal	693:699	arg1	structure					801:809	the first atomic-level structure	778:809	the first atomic-level structure of any betaherpesvirus glycoprotein	778:845	Here, we report the crystal structure of the HCMV gB ectodomain determined to 3.6-Å resolution, which is the first atomic-level structure of any betaherpesvirus glycoprotein.
26484870	1	70	theme	congenital	335:344	arg1	infections					352:361	congenital viral infections	335:361	congenital viral infections	335:361	HCMV is also a leading cause of congenital viral infections in newborns.
26484870	0	71	theme	immune	235:240	arg1	systems					242:248	compromised immune systems	223:248	compromised immune systems	223:248	Crystal Structure of the Human Cytomegalovirus Glycoprotein B. Human cytomegalovirus (HCMV), a dsDNA, enveloped virus, is a ubiquitous pathogen that establishes lifelong latent infections and caused disease in persons with compromised immune systems, e.g., organ transplant recipients or AIDS patients.
26484870	1	72	theme	infections	352:361	arg1	cause					326:330	a leading cause	316:330	a leading cause of congenital viral infections in newborns	316:373	HCMV is also a leading cause of congenital viral infections in newborns.
26484870	1	72	theme	infections	352:361	arg1	HCMV					303:306	HCMV	303:306	HCMV	303:306	HCMV is also a leading cause of congenital viral infections in newborns.
26484870	5	73	theme	postfusion	887:896	arg1	structures					898:907	the postfusion structures	883:907	the postfusion structures of HSV-1 and EBV homologs	883:933	The structure of HCMV gB resembles the postfusion structures of HSV-1 and EBV homologs, establishing it as a new member of the class III viral fusogens.
26484870	7	74	theme	antibody	1264:1271	arg1	recognition					1273:1283	antibody recognition	1264:1283	antibody recognition	1264:1283	The structure illustrates how extensive glycosylation of the gB ectodomain influences antibody recognition.
26484870	10	75	theme	gB	1701:1702	arg1	structure					1704:1712	HCMV gB structure	1696:1712	HCMV gB structure	1696:1712	HCMV gB structure provides a starting point for elucidation of its antigenic and immunogenic properties and aid in the design of recombinant vaccines and monoclonal antibody therapies.
26484870	0	76	theme	Cytomegalovirus	31:45	arg1	Glycoprotein					47:58	the Human Cytomegalovirus Glycoprotein B. Human cytomegalovirus (HCMV), a dsDNA, enveloped virus,	21:117	the Human Cytomegalovirus Glycoprotein B. Human cytomegalovirus (HCMV), a dsDNA, enveloped virus,	21:117	Crystal Structure of the Human Cytomegalovirus Glycoprotein B. Human cytomegalovirus (HCMV), a dsDNA, enveloped virus, is a ubiquitous pathogen that establishes lifelong latent infections and caused disease in persons with compromised immune systems, e.g., organ transplant recipients or AIDS patients.
26484870	7	77	theme	ectodomain	1242:1251	arg1	glycosylation					1218:1230	extensive glycosylation	1208:1230	extensive glycosylation of the gB ectodomain	1208:1251	The structure illustrates how extensive glycosylation of the gB ectodomain influences antibody recognition.
26484870	0	78	theme	B.	60:61	arg1	virus					112:116	enveloped virus	102:116	enveloped virus	102:116	Crystal Structure of the Human Cytomegalovirus Glycoprotein B. Human cytomegalovirus (HCMV), a dsDNA, enveloped virus, is a ubiquitous pathogen that establishes lifelong latent infections and caused disease in persons with compromised immune systems, e.g., organ transplant recipients or AIDS patients.
26484870	0	78	theme	B.	60:61	arg1	HCMV					86:89	HCMV	86:89	HCMV	86:89	Crystal Structure of the Human Cytomegalovirus Glycoprotein B. Human cytomegalovirus (HCMV), a dsDNA, enveloped virus, is a ubiquitous pathogen that establishes lifelong latent infections and caused disease in persons with compromised immune systems, e.g., organ transplant recipients or AIDS patients.
26484870	0	78	theme	B.	60:61	arg1	cytomegalovirus					69:83	B. Human cytomegalovirus	60:83	B. Human cytomegalovirus (HCMV)	60:90	Crystal Structure of the Human Cytomegalovirus Glycoprotein B. Human cytomegalovirus (HCMV), a dsDNA, enveloped virus, is a ubiquitous pathogen that establishes lifelong latent infections and caused disease in persons with compromised immune systems, e.g., organ transplant recipients or AIDS patients.
26484870	0	78	theme	B.	60:61	arg1	dsDNA					95:99	a dsDNA	93:99	a dsDNA	93:99	Crystal Structure of the Human Cytomegalovirus Glycoprotein B. Human cytomegalovirus (HCMV), a dsDNA, enveloped virus, is a ubiquitous pathogen that establishes lifelong latent infections and caused disease in persons with compromised immune systems, e.g., organ transplant recipients or AIDS patients.
26484870	5	79	theme	new	957:959	arg1	it					949:950	it	949:950	it	949:950	The structure of HCMV gB resembles the postfusion structures of HSV-1 and EBV homologs, establishing it as a new member of the class III viral fusogens.
26484870	5	79	theme	new	957:959	arg1	member					961:966	a new member	955:966	a new member of the class III viral fusogens	955:998	The structure of HCMV gB resembles the postfusion structures of HSV-1 and EBV homologs, establishing it as a new member of the class III viral fusogens.
26484870	6	80	theme	unique	1048:1053	arg1	arrangement					1062:1072	a unique domain arrangement	1046:1072	a unique domain arrangement	1046:1072	Despite structural similarities, each gB has a unique domain arrangement, demonstrating structural plasticity of gB that may accommodate virus-specific functional requirements.
26484870	2	81	theme	conserved	414:422	arg1	gB					440:441	gB	440:441	gB	440:441	Entry of HCMV into cells requires the conserved glycoprotein B (gB), thought to function as a fusogen and reported to bind signaling receptors.
26484870	2	81	theme	conserved	414:422	arg1	B					437:437	glycoprotein B	424:437	the conserved glycoprotein B (gB)	410:442	Entry of HCMV into cells requires the conserved glycoprotein B (gB), thought to function as a fusogen and reported to bind signaling receptors.
26484870	3	82	theme	neutralizing	601:612	arg1	antibodies					614:623	neutralizing antibodies	601:623	neutralizing antibodies	601:623	gB also elicits a strong immune response in humans and induces the production of neutralizing antibodies although most anti-gB Abs are non-neutralizing.
26484870	10	83	theme	starting	1725:1732	arg1	point					1734:1738	a starting point	1723:1738	a starting point for elucidation of its antigenic and immunogenic properties	1723:1798	HCMV gB structure provides a starting point for elucidation of its antigenic and immunogenic properties and aid in the design of recombinant vaccines and monoclonal antibody therapies.
26484870	5	84	theme	homologs	926:933	arg1	structures					898:907	the postfusion structures	883:907	the postfusion structures of HSV-1 and EBV homologs	883:933	The structure of HCMV gB resembles the postfusion structures of HSV-1 and EBV homologs, establishing it as a new member of the class III viral fusogens.
26484870	4	85	theme	HCMV	718:721	arg1	ectodomain					726:735	the HCMV gB ectodomain	714:735	the HCMV gB ectodomain determined to 3.6-Å resolution	714:766	Here, we report the crystal structure of the HCMV gB ectodomain determined to 3.6-Å resolution, which is the first atomic-level structure of any betaherpesvirus glycoprotein.
26484870	3	86	theme	anti-gB	639:645	arg1	Abs					647:649	most anti-gB Abs	634:649	most anti-gB Abs	634:649	gB also elicits a strong immune response in humans and induces the production of neutralizing antibodies although most anti-gB Abs are non-neutralizing.
26484870	4	87	theme	ectodomain	726:735	arg1	structure					701:709	the crystal structure	689:709	the crystal structure	689:709	Here, we report the crystal structure of the HCMV gB ectodomain determined to 3.6-Å resolution, which is the first atomic-level structure of any betaherpesvirus glycoprotein.
26484870	4	87	theme	ectodomain	726:735	arg1	structure					801:809	the first atomic-level structure	778:809	the first atomic-level structure of any betaherpesvirus glycoprotein	778:845	Here, we report the crystal structure of the HCMV gB ectodomain determined to 3.6-Å resolution, which is the first atomic-level structure of any betaherpesvirus glycoprotein.
26196117	7	0	theme	sulfate	1328:1334	arg1	ester					1336:1340	the side chain sulfate ester	1313:1340	the side chain sulfate ester	1313:1340	Initial synthetic studies used a cassette-based solid-phase peptide synthesis (SPPS) approach in which the side chain sulfate ester was protected to enable it to withstand Fmoc-based SPPS conditions.
26196117	3	1	theme	heterogeneous	717:729	arg1	mixtures					731:738	heterogeneous mixtures	717:738	heterogeneous mixtures of sulfoforms	717:752	Tyrosine sulfation commonly occurs in sequences containing clusters of tyrosine residues and is incomplete at each site, resulting in heterogeneous mixtures of sulfoforms.
26196117	7	2	theme	synthetic	1218:1226	arg1	studies					1228:1234	Initial synthetic studies	1210:1234	Initial synthetic studies	1210:1234	Initial synthetic studies used a cassette-based solid-phase peptide synthesis (SPPS) approach in which the side chain sulfate ester was protected to enable it to withstand Fmoc-based SPPS conditions.
26196117	15	3	theme	different	2967:2975	arg1	modifications					2996:3008	different post-translational modifications	2967:3008	different post-translational modifications	2967:3008	Sulfation greatly enhanced inhibitory activity against thrombin, whereas addition of glycans to the sulfated protein decreased inhibition, indicating functional interplay between different post-translational modifications.
26196117	2	4	theme	side	313:316	arg1	chain					318:322	the phenolic side chain	300:322	the phenolic side chain of tyrosine residues to form sulfotyrosine (sTyr)	300:372	Sulfation of the phenolic side chain of tyrosine residues to form sulfotyrosine (sTyr) is a widespread modification of extracellular and integral membrane proteins, influencing the activities of these proteins in cellular adhesion, blood clotting, inflammatory responses, and pathogen infection.
26196117	5	5	dep	approach	849:856	arg1	elucidate					861:869	elucidate	861:869	to elucidate the influence of sulfation at each site	858:909	Therefore, the most promising approach to elucidate the influence of sulfation at each site is to prepare homogeneously sulfated proteins (or peptides) synthetically.
26196117	15	6	theme	functional	2938:2947	arg1	interplay					2949:2957	functional interplay	2938:2957	functional interplay between different post-translational modifications	2938:3008	Sulfation greatly enhanced inhibitory activity against thrombin, whereas addition of glycans to the sulfated protein decreased inhibition, indicating functional interplay between different post-translational modifications.
26196117	11	7	theme	leukocyte	2093:2101	arg1	trafficking					2103:2113	leukocyte trafficking	2093:2113	leukocyte trafficking	2093:2113	Several of these studies focused on sulfated fragments of chemokine receptors, key mediators of leukocyte trafficking and inflammation.
26196117	1	8	theme	critical	208:215	arg1	roles					217:221	critical roles	208:221	critical roles	208:221	Post-translational modification of proteins plays critical roles in regulating structure, stability, localization, and function.
26196117	12	9	theme	chemokine	2239:2247	arg1	ligands					2249:2255	chemokine ligands	2239:2255	chemokine ligands	2239:2255	For the receptor CCR3, we showed that tyrosine sulfation enhances affinity and selectivity for binding to chemokine ligands, and we determined the structural basis of these affinity enhancements by NMR spectroscopy.
26196117	16	10	theme	synthetic	3141:3149	arg1	sulfopeptides					3151:3163	synthetic sulfopeptides	3141:3163	synthetic sulfopeptides	3141:3163	In addition, the success of the ligation approach suggests that larger sulfoproteins could potentially be obtained by ligation of synthetic sulfopeptides to expressed proteins, using intein-based technology.
26196117	2	11	theme	residues	336:343	arg1	chain					318:322	the phenolic side chain	300:322	the phenolic side chain of tyrosine residues to form sulfotyrosine (sTyr)	300:372	Sulfation of the phenolic side chain of tyrosine residues to form sulfotyrosine (sTyr) is a widespread modification of extracellular and integral membrane proteins, influencing the activities of these proteins in cellular adhesion, blood clotting, inflammatory responses, and pathogen infection.
26196117	15	12	theme	post-translational	2977:2994	arg1	modifications					2996:3008	different post-translational modifications	2967:3008	different post-translational modifications	2967:3008	Sulfation greatly enhanced inhibitory activity against thrombin, whereas addition of glycans to the sulfated protein decreased inhibition, indicating functional interplay between different post-translational modifications.
26196117	8	13	theme	efficient	1448:1456	arg1	access					1458:1463	efficient access	1448:1463	efficient access to multiple sulfoforms of the same peptide	1448:1506	Subsequently, to address the need for efficient access to multiple sulfoforms of the same peptide, we developed a divergent solid-phase synthetic approach utilizing orthogonally side chain protected tyrosine residues.
26196117	6	14	theme	recent	1013:1018	arg1	progress					1020:1027	our recent progress	1009:1027	our recent progress in both development of such synthetic approaches and application of the resulting sulfopeptides and sulfoproteins to characterize the functional consequences of tyrosine sulfation	1009:1207	This Account describes our recent progress in both development of such synthetic approaches and application of the resulting sulfopeptides and sulfoproteins to characterize the functional consequences of tyrosine sulfation.
26196117	2	15	theme	pathogen	563:570	arg1	infection					572:580	pathogen infection	563:580	pathogen infection	563:580	Sulfation of the phenolic side chain of tyrosine residues to form sulfotyrosine (sTyr) is a widespread modification of extracellular and integral membrane proteins, influencing the activities of these proteins in cellular adhesion, blood clotting, inflammatory responses, and pathogen infection.
26196117	14	16	theme	protein	2714:2720	arg1	P6					2730:2731	the leech-derived antithrombotic protein hirudin P6	2681:2731	the leech-derived antithrombotic protein hirudin P6	2681:2731	Demonstrating the feasibility of producing homogeneously tyrosine-sulfated proteins, in addition to smaller peptides, we have used SPPS and native chemical ligation methods to synthesize the leech-derived antithrombotic protein hirudin P6, containing both tyrosine sulfation and glycosylation.
26196117	9	17	dep	three	1719:1723	arg1	to					1716:1717	to	1716:1717	to	1716:1717	Using this methodology, we have carried out orthogonal deprotection and sulfation of up to three tyrosine residues within a given sequence, allowing access to all eight sulfoforms of a given target from a single solid-phase synthesis.
26196117	16	18	theme	approach	3052:3059	arg1	success					3028:3034	the success	3024:3034	the success of the ligation approach	3024:3059	In addition, the success of the ligation approach suggests that larger sulfoproteins could potentially be obtained by ligation of synthetic sulfopeptides to expressed proteins, using intein-based technology.
26196117	11	19	theme	chemokine	2055:2063	arg1	receptors					2065:2073	chemokine receptors	2055:2073	chemokine receptors	2055:2073	Several of these studies focused on sulfated fragments of chemokine receptors, key mediators of leukocyte trafficking and inflammation.
26196117	14	20	theme	tyrosine	2750:2757	arg1	sulfation					2759:2767	tyrosine sulfation	2750:2767	tyrosine sulfation	2750:2767	Demonstrating the feasibility of producing homogeneously tyrosine-sulfated proteins, in addition to smaller peptides, we have used SPPS and native chemical ligation methods to synthesize the leech-derived antithrombotic protein hirudin P6, containing both tyrosine sulfation and glycosylation.
26196117	9	21	theme	given	1813:1817	arg1	target					1819:1824	a given target	1811:1824	a given target from a single solid-phase synthesis	1811:1860	Using this methodology, we have carried out orthogonal deprotection and sulfation of up to three tyrosine residues within a given sequence, allowing access to all eight sulfoforms of a given target from a single solid-phase synthesis.
26196117	8	22	theme	solid-phase	1534:1544	arg1	approach					1556:1563	a divergent solid-phase synthetic approach	1522:1563	a divergent solid-phase synthetic approach utilizing orthogonally side chain protected tyrosine residues	1522:1625	Subsequently, to address the need for efficient access to multiple sulfoforms of the same peptide, we developed a divergent solid-phase synthetic approach utilizing orthogonally side chain protected tyrosine residues.
26196117	13	23	theme	sulfopeptides	2373:2385	arg1	library					2357:2363	a library	2355:2363	a library of CCR5 sulfopeptides	2355:2385	Using a library of CCR5 sulfopeptides, we demonstrated the critical importance of sulfation at one specific site for supporting HIV-1 infection.
26196117	10	24	with	able	1922:1925	arg1	peptides					1891:1898	homogeneously sulfated peptides	1868:1898	homogeneously sulfated peptides in hand	1868:1906	With homogeneously sulfated peptides in hand, we have been able to probe the influence of tyrosine sulfation on biochemical function.
26196117	11	25	theme	key	2076:2078	arg1	fragments					2042:2050	sulfated fragments	2033:2050	sulfated fragments of chemokine receptors	2033:2073	Several of these studies focused on sulfated fragments of chemokine receptors, key mediators of leukocyte trafficking and inflammation.
26196117	11	25	theme	key	2076:2078	arg1	mediators					2080:2088	key mediators	2076:2088	key mediators of leukocyte trafficking and inflammation	2076:2130	Several of these studies focused on sulfated fragments of chemokine receptors, key mediators of leukocyte trafficking and inflammation.
26196117	4	26	theme	individual	771:780	arg1	sulfoforms					782:791	individual sulfoforms	771:791	individual sulfoforms	771:791	Purification of individual sulfoforms is typically impractical.
26196117	12	27	theme	enhancements	2315:2326	arg1	basis					2291:2295	the structural basis	2276:2295	the structural basis of these affinity enhancements	2276:2326	For the receptor CCR3, we showed that tyrosine sulfation enhances affinity and selectivity for binding to chemokine ligands, and we determined the structural basis of these affinity enhancements by NMR spectroscopy.
26196117	11	28	theme	trafficking	2103:2113	arg1	fragments					2042:2050	sulfated fragments	2033:2050	sulfated fragments of chemokine receptors	2033:2073	Several of these studies focused on sulfated fragments of chemokine receptors, key mediators of leukocyte trafficking and inflammation.
26196117	11	28	theme	trafficking	2103:2113	arg1	mediators					2080:2088	key mediators	2076:2088	key mediators of leukocyte trafficking and inflammation	2076:2130	Several of these studies focused on sulfated fragments of chemokine receptors, key mediators of leukocyte trafficking and inflammation.
26196117	9	29	theme	single	1833:1838	arg1	synthesis					1852:1860	a single solid-phase synthesis	1831:1860	a single solid-phase synthesis	1831:1860	Using this methodology, we have carried out orthogonal deprotection and sulfation of up to three tyrosine residues within a given sequence, allowing access to all eight sulfoforms of a given target from a single solid-phase synthesis.
26196117	0	30	from	effects	103:109	arg1	function					148:155	biochemical function	136:155	biochemical function	136:155	Homogeneous sulfopeptides and sulfoproteins: synthetic approaches and applications to characterize the effects of tyrosine sulfation on biochemical function.
26196117	14	31	contain	containing	2734:2743	arg2	sulfation					2759:2767	tyrosine sulfation	2750:2767	tyrosine sulfation	2750:2767	Demonstrating the feasibility of producing homogeneously tyrosine-sulfated proteins, in addition to smaller peptides, we have used SPPS and native chemical ligation methods to synthesize the leech-derived antithrombotic protein hirudin P6, containing both tyrosine sulfation and glycosylation.
26196117	14	31	contain	containing	2734:2743	arg2	glycosylation					2773:2785	glycosylation	2773:2785	glycosylation	2773:2785	Demonstrating the feasibility of producing homogeneously tyrosine-sulfated proteins, in addition to smaller peptides, we have used SPPS and native chemical ligation methods to synthesize the leech-derived antithrombotic protein hirudin P6, containing both tyrosine sulfation and glycosylation.
26196117	14	31	contain	containing	2734:2743	arg1	P6					2730:2731	the leech-derived antithrombotic protein hirudin P6	2681:2731	the leech-derived antithrombotic protein hirudin P6	2681:2731	Demonstrating the feasibility of producing homogeneously tyrosine-sulfated proteins, in addition to smaller peptides, we have used SPPS and native chemical ligation methods to synthesize the leech-derived antithrombotic protein hirudin P6, containing both tyrosine sulfation and glycosylation.
26196117	7	32	theme	chain	1322:1326	arg1	ester					1336:1340	the side chain sulfate ester	1313:1340	the side chain sulfate ester	1313:1340	Initial synthetic studies used a cassette-based solid-phase peptide synthesis (SPPS) approach in which the side chain sulfate ester was protected to enable it to withstand Fmoc-based SPPS conditions.
26196117	3	33	theme	tyrosine	654:661	arg1	residues					663:670	tyrosine residues	654:670	tyrosine residues	654:670	Tyrosine sulfation commonly occurs in sequences containing clusters of tyrosine residues and is incomplete at each site, resulting in heterogeneous mixtures of sulfoforms.
26196117	14	34	link	leech-derived	2685:2697	arg1	P6					2730:2731	the leech-derived antithrombotic protein hirudin P6	2681:2731	the leech-derived antithrombotic protein hirudin P6	2681:2731	Demonstrating the feasibility of producing homogeneously tyrosine-sulfated proteins, in addition to smaller peptides, we have used SPPS and native chemical ligation methods to synthesize the leech-derived antithrombotic protein hirudin P6, containing both tyrosine sulfation and glycosylation.
26196117	6	35	theme	approaches	1067:1076	arg1	development					1037:1047	development	1037:1047	development of such synthetic approaches	1037:1076	This Account describes our recent progress in both development of such synthetic approaches and application of the resulting sulfopeptides and sulfoproteins to characterize the functional consequences of tyrosine sulfation.
26196117	6	35	theme	approaches	1067:1076	arg1	application					1082:1092	application	1082:1092	application of the resulting sulfopeptides and sulfoproteins to characterize the functional consequences of tyrosine sulfation	1082:1207	This Account describes our recent progress in both development of such synthetic approaches and application of the resulting sulfopeptides and sulfoproteins to characterize the functional consequences of tyrosine sulfation.
26196117	8	36	theme	chain	1593:1597	arg1	residues					1618:1625	orthogonally side chain protected tyrosine residues	1575:1625	orthogonally side chain protected tyrosine residues	1575:1625	Subsequently, to address the need for efficient access to multiple sulfoforms of the same peptide, we developed a divergent solid-phase synthetic approach utilizing orthogonally side chain protected tyrosine residues.
26196117	1	37	theme	proteins	193:200	arg1	modification					177:188	Post-translational modification	158:188	Post-translational modification of proteins	158:200	Post-translational modification of proteins plays critical roles in regulating structure, stability, localization, and function.
26196117	10	38	from	peptides	1891:1898	arg1	hand					1903:1906	hand	1903:1906	hand	1903:1906	With homogeneously sulfated peptides in hand, we have been able to probe the influence of tyrosine sulfation on biochemical function.
26196117	6	39	theme	such	1052:1055	arg1	approaches					1067:1076	such synthetic approaches	1052:1076	such synthetic approaches	1052:1076	This Account describes our recent progress in both development of such synthetic approaches and application of the resulting sulfopeptides and sulfoproteins to characterize the functional consequences of tyrosine sulfation.
26196117	2	40	from	activities	468:477	arg1	infection					572:580	pathogen infection	563:580	pathogen infection	563:580	Sulfation of the phenolic side chain of tyrosine residues to form sulfotyrosine (sTyr) is a widespread modification of extracellular and integral membrane proteins, influencing the activities of these proteins in cellular adhesion, blood clotting, inflammatory responses, and pathogen infection.
26196117	2	40	from	activities	468:477	arg1	adhesion					509:516	cellular adhesion	500:516	cellular adhesion	500:516	Sulfation of the phenolic side chain of tyrosine residues to form sulfotyrosine (sTyr) is a widespread modification of extracellular and integral membrane proteins, influencing the activities of these proteins in cellular adhesion, blood clotting, inflammatory responses, and pathogen infection.
26196117	2	40	from	activities	468:477	arg1	clotting					525:532	blood clotting	519:532	blood clotting	519:532	Sulfation of the phenolic side chain of tyrosine residues to form sulfotyrosine (sTyr) is a widespread modification of extracellular and integral membrane proteins, influencing the activities of these proteins in cellular adhesion, blood clotting, inflammatory responses, and pathogen infection.
26196117	2	40	from	activities	468:477	arg1	responses					548:556	inflammatory responses	535:556	inflammatory responses	535:556	Sulfation of the phenolic side chain of tyrosine residues to form sulfotyrosine (sTyr) is a widespread modification of extracellular and integral membrane proteins, influencing the activities of these proteins in cellular adhesion, blood clotting, inflammatory responses, and pathogen infection.
26196117	12	41	theme	NMR	2331:2333	arg1	spectroscopy					2335:2346	NMR spectroscopy	2331:2346	NMR spectroscopy	2331:2346	For the receptor CCR3, we showed that tyrosine sulfation enhances affinity and selectivity for binding to chemokine ligands, and we determined the structural basis of these affinity enhancements by NMR spectroscopy.
26196117	9	42	from	sulfoforms	1797:1806	arg1	synthesis					1852:1860	a single solid-phase synthesis	1831:1860	a single solid-phase synthesis	1831:1860	Using this methodology, we have carried out orthogonal deprotection and sulfation of up to three tyrosine residues within a given sequence, allowing access to all eight sulfoforms of a given target from a single solid-phase synthesis.
26196117	2	43	theme	inflammatory	535:546	arg1	responses					548:556	inflammatory responses	535:556	inflammatory responses	535:556	Sulfation of the phenolic side chain of tyrosine residues to form sulfotyrosine (sTyr) is a widespread modification of extracellular and integral membrane proteins, influencing the activities of these proteins in cellular adhesion, blood clotting, inflammatory responses, and pathogen infection.
26196117	0	44	theme	tyrosine	114:121	arg1	sulfation					123:131	tyrosine sulfation	114:131	tyrosine sulfation	114:131	Homogeneous sulfopeptides and sulfoproteins: synthetic approaches and applications to characterize the effects of tyrosine sulfation on biochemical function.
26196117	15	45	theme	glycans	2873:2879	arg1	addition					2861:2868	addition	2861:2868	addition of glycans to the sulfated protein	2861:2903	Sulfation greatly enhanced inhibitory activity against thrombin, whereas addition of glycans to the sulfated protein decreased inhibition, indicating functional interplay between different post-translational modifications.
26196117	16	46	theme	ligation	3043:3050	arg1	approach					3052:3059	the ligation approach	3039:3059	the ligation approach	3039:3059	In addition, the success of the ligation approach suggests that larger sulfoproteins could potentially be obtained by ligation of synthetic sulfopeptides to expressed proteins, using intein-based technology.
26196117	11	47	theme	studies	2014:2020	arg1	Several					1997:2003	Several	1997:2003	Several	1997:2003	Several of these studies focused on sulfated fragments of chemokine receptors, key mediators of leukocyte trafficking and inflammation.
26196117	13	48	from	site	2457:2460	arg1	sulfation					2431:2439	sulfation	2431:2439	sulfation at one specific site for supporting HIV-1 infection	2431:2491	Using a library of CCR5 sulfopeptides, we demonstrated the critical importance of sulfation at one specific site for supporting HIV-1 infection.
26196117	13	48	from	site	2457:2460	arg1	importance					2417:2426	the critical importance	2404:2426	the critical importance of sulfation at one specific site for supporting HIV-1 infection	2404:2491	Using a library of CCR5 sulfopeptides, we demonstrated the critical importance of sulfation at one specific site for supporting HIV-1 infection.
26196117	0	49	theme	Homogeneous	0:10	arg1	sulfopeptides					12:24	Homogeneous sulfopeptides	0:24	Homogeneous sulfopeptides	0:24	Homogeneous sulfopeptides and sulfoproteins: synthetic approaches and applications to characterize the effects of tyrosine sulfation on biochemical function.
26196117	9	50	theme	orthogonal	1672:1681	arg1	deprotection					1683:1694	orthogonal deprotection	1672:1694	orthogonal deprotection	1672:1694	Using this methodology, we have carried out orthogonal deprotection and sulfation of up to three tyrosine residues within a given sequence, allowing access to all eight sulfoforms of a given target from a single solid-phase synthesis.
26196117	6	51	theme	sulfoproteins	1129:1141	arg1	development					1037:1047	development	1037:1047	development of such synthetic approaches	1037:1076	This Account describes our recent progress in both development of such synthetic approaches and application of the resulting sulfopeptides and sulfoproteins to characterize the functional consequences of tyrosine sulfation.
26196117	6	51	theme	sulfoproteins	1129:1141	arg1	application					1082:1092	application	1082:1092	application of the resulting sulfopeptides and sulfoproteins to characterize the functional consequences of tyrosine sulfation	1082:1207	This Account describes our recent progress in both development of such synthetic approaches and application of the resulting sulfopeptides and sulfoproteins to characterize the functional consequences of tyrosine sulfation.
26196117	16	52	theme	intein-based	3194:3205	arg1	technology					3207:3216	intein-based technology	3194:3216	intein-based technology	3194:3216	In addition, the success of the ligation approach suggests that larger sulfoproteins could potentially be obtained by ligation of synthetic sulfopeptides to expressed proteins, using intein-based technology.
26196117	6	53	from	progress	1020:1027	arg1	development					1037:1047	development	1037:1047	development of such synthetic approaches	1037:1076	This Account describes our recent progress in both development of such synthetic approaches and application of the resulting sulfopeptides and sulfoproteins to characterize the functional consequences of tyrosine sulfation.
26196117	6	53	from	progress	1020:1027	arg1	application					1082:1092	application	1082:1092	application of the resulting sulfopeptides and sulfoproteins to characterize the functional consequences of tyrosine sulfation	1082:1207	This Account describes our recent progress in both development of such synthetic approaches and application of the resulting sulfopeptides and sulfoproteins to characterize the functional consequences of tyrosine sulfation.
26196117	8	54	theme	multiple	1468:1475	arg1	sulfoforms					1477:1486	multiple sulfoforms	1468:1486	multiple sulfoforms of the same peptide	1468:1506	Subsequently, to address the need for efficient access to multiple sulfoforms of the same peptide, we developed a divergent solid-phase synthetic approach utilizing orthogonally side chain protected tyrosine residues.
26196117	7	55	theme	peptide	1270:1276	arg1	approach					1295:1302	a cassette-based solid-phase peptide synthesis (SPPS) approach	1241:1302	a cassette-based solid-phase peptide synthesis (SPPS) approach in which the side chain sulfate ester was protected to enable it to withstand Fmoc-based SPPS conditions	1241:1407	Initial synthetic studies used a cassette-based solid-phase peptide synthesis (SPPS) approach in which the side chain sulfate ester was protected to enable it to withstand Fmoc-based SPPS conditions.
26196117	6	56	theme	sulfopeptides	1111:1123	arg1	development					1037:1047	development	1037:1047	development of such synthetic approaches	1037:1076	This Account describes our recent progress in both development of such synthetic approaches and application of the resulting sulfopeptides and sulfoproteins to characterize the functional consequences of tyrosine sulfation.
26196117	6	56	theme	sulfopeptides	1111:1123	arg1	application					1082:1092	application	1082:1092	application of the resulting sulfopeptides and sulfoproteins to characterize the functional consequences of tyrosine sulfation	1082:1207	This Account describes our recent progress in both development of such synthetic approaches and application of the resulting sulfopeptides and sulfoproteins to characterize the functional consequences of tyrosine sulfation.
26196117	2	57	theme	cellular	500:507	arg1	adhesion					509:516	cellular adhesion	500:516	cellular adhesion	500:516	Sulfation of the phenolic side chain of tyrosine residues to form sulfotyrosine (sTyr) is a widespread modification of extracellular and integral membrane proteins, influencing the activities of these proteins in cellular adhesion, blood clotting, inflammatory responses, and pathogen infection.
26196117	2	58	theme	membrane	433:440	arg1	proteins					442:449	extracellular and integral membrane proteins	406:449	proteins	442:449	Sulfation of the phenolic side chain of tyrosine residues to form sulfotyrosine (sTyr) is a widespread modification of extracellular and integral membrane proteins, influencing the activities of these proteins in cellular adhesion, blood clotting, inflammatory responses, and pathogen infection.
26196117	5	59	theme	promising	839:847	arg1	approach					849:856	the most promising approach	830:856	the most promising approach to elucidate the influence of sulfation at each site	830:909	Therefore, the most promising approach to elucidate the influence of sulfation at each site is to prepare homogeneously sulfated proteins (or peptides) synthetically.
26196117	7	60	theme	cassette-based	1243:1256	arg1	approach					1295:1302	a cassette-based solid-phase peptide synthesis (SPPS) approach	1241:1302	a cassette-based solid-phase peptide synthesis (SPPS) approach in which the side chain sulfate ester was protected to enable it to withstand Fmoc-based SPPS conditions	1241:1407	Initial synthetic studies used a cassette-based solid-phase peptide synthesis (SPPS) approach in which the side chain sulfate ester was protected to enable it to withstand Fmoc-based SPPS conditions.
26196117	14	61	theme	ligation	2650:2657	arg1	methods					2659:2665	SPPS and native chemical ligation methods	2625:2665	SPPS and native chemical ligation methods	2625:2665	Demonstrating the feasibility of producing homogeneously tyrosine-sulfated proteins, in addition to smaller peptides, we have used SPPS and native chemical ligation methods to synthesize the leech-derived antithrombotic protein hirudin P6, containing both tyrosine sulfation and glycosylation.
26196117	7	62	theme	Fmoc-based	1382:1391	arg1	conditions					1398:1407	Fmoc-based SPPS conditions	1382:1407	Fmoc-based SPPS conditions	1382:1407	Initial synthetic studies used a cassette-based solid-phase peptide synthesis (SPPS) approach in which the side chain sulfate ester was protected to enable it to withstand Fmoc-based SPPS conditions.
26196117	2	63	theme	proteins	488:495	arg1	activities					468:477	the activities	464:477	the activities of these proteins in cellular adhesion, blood clotting, inflammatory responses, and pathogen infection	464:580	Sulfation of the phenolic side chain of tyrosine residues to form sulfotyrosine (sTyr) is a widespread modification of extracellular and integral membrane proteins, influencing the activities of these proteins in cellular adhesion, blood clotting, inflammatory responses, and pathogen infection.
26196117	2	64	theme	blood	519:523	arg1	clotting					525:532	blood clotting	519:532	blood clotting	519:532	Sulfation of the phenolic side chain of tyrosine residues to form sulfotyrosine (sTyr) is a widespread modification of extracellular and integral membrane proteins, influencing the activities of these proteins in cellular adhesion, blood clotting, inflammatory responses, and pathogen infection.
26196117	8	65	theme	same	1495:1498	arg1	peptide					1500:1506	the same peptide	1491:1506	the same peptide	1491:1506	Subsequently, to address the need for efficient access to multiple sulfoforms of the same peptide, we developed a divergent solid-phase synthetic approach utilizing orthogonally side chain protected tyrosine residues.
26196117	13	66	from	importance	2417:2426	arg1	site					2457:2460	one specific site	2444:2460	one specific site for supporting HIV-1 infection	2444:2491	Using a library of CCR5 sulfopeptides, we demonstrated the critical importance of sulfation at one specific site for supporting HIV-1 infection.
26196117	9	67	from	synthesis	1852:1860	arg1	target					1819:1824	a given target	1811:1824	a given target from a single solid-phase synthesis	1811:1860	Using this methodology, we have carried out orthogonal deprotection and sulfation of up to three tyrosine residues within a given sequence, allowing access to all eight sulfoforms of a given target from a single solid-phase synthesis.
26196117	9	67	from	synthesis	1852:1860	arg1	sulfoforms					1797:1806	all eight sulfoforms	1787:1806	all eight sulfoforms of a given target from a single solid-phase synthesis	1787:1860	Using this methodology, we have carried out orthogonal deprotection and sulfation of up to three tyrosine residues within a given sequence, allowing access to all eight sulfoforms of a given target from a single solid-phase synthesis.
26196117	14	68	theme	SPPS	2625:2628	arg1	methods					2659:2665	SPPS and native chemical ligation methods	2625:2665	SPPS and native chemical ligation methods	2625:2665	Demonstrating the feasibility of producing homogeneously tyrosine-sulfated proteins, in addition to smaller peptides, we have used SPPS and native chemical ligation methods to synthesize the leech-derived antithrombotic protein hirudin P6, containing both tyrosine sulfation and glycosylation.
26196117	9	69	theme	tyrosine	1725:1732	arg1	residues					1734:1741	up to three tyrosine residues	1713:1741	up to three tyrosine residues within a given sequence	1713:1765	Using this methodology, we have carried out orthogonal deprotection and sulfation of up to three tyrosine residues within a given sequence, allowing access to all eight sulfoforms of a given target from a single solid-phase synthesis.
26196117	6	70	theme	sulfation	1199:1207	arg1	consequences					1174:1185	the functional consequences	1159:1185	the functional consequences of tyrosine sulfation	1159:1207	This Account describes our recent progress in both development of such synthetic approaches and application of the resulting sulfopeptides and sulfoproteins to characterize the functional consequences of tyrosine sulfation.
26196117	3	71	from	site	698:701	arg1	incomplete					679:688	incomplete	679:688	incomplete	679:688	Tyrosine sulfation commonly occurs in sequences containing clusters of tyrosine residues and is incomplete at each site, resulting in heterogeneous mixtures of sulfoforms.
26196117	10	72	theme	tyrosine	1953:1960	arg1	sulfation					1962:1970	tyrosine sulfation	1953:1970	tyrosine sulfation	1953:1970	With homogeneously sulfated peptides in hand, we have been able to probe the influence of tyrosine sulfation on biochemical function.
26196117	2	73	theme	widespread	379:388	arg1	modification					390:401	a widespread modification	377:401	a widespread modification of extracellular and integral membrane proteins, influencing the activities of these proteins in cellular adhesion, blood clotting, inflammatory responses, and pathogen infection	377:580	Sulfation of the phenolic side chain of tyrosine residues to form sulfotyrosine (sTyr) is a widespread modification of extracellular and integral membrane proteins, influencing the activities of these proteins in cellular adhesion, blood clotting, inflammatory responses, and pathogen infection.
26196117	2	73	theme	widespread	379:388	arg1	Sulfation					287:295	Sulfation	287:295	Sulfation of the phenolic side chain of tyrosine residues to form sulfotyrosine (sTyr)	287:372	Sulfation of the phenolic side chain of tyrosine residues to form sulfotyrosine (sTyr) is a widespread modification of extracellular and integral membrane proteins, influencing the activities of these proteins in cellular adhesion, blood clotting, inflammatory responses, and pathogen infection.
26196117	13	74	theme	sulfation	2431:2439	arg1	importance					2417:2426	the critical importance	2404:2426	the critical importance of sulfation at one specific site for supporting HIV-1 infection	2404:2491	Using a library of CCR5 sulfopeptides, we demonstrated the critical importance of sulfation at one specific site for supporting HIV-1 infection.
26196117	7	75	theme	SPPS	1289:1292	arg1	approach					1295:1302	a cassette-based solid-phase peptide synthesis (SPPS) approach	1241:1302	a cassette-based solid-phase peptide synthesis (SPPS) approach in which the side chain sulfate ester was protected to enable it to withstand Fmoc-based SPPS conditions	1241:1407	Initial synthetic studies used a cassette-based solid-phase peptide synthesis (SPPS) approach in which the side chain sulfate ester was protected to enable it to withstand Fmoc-based SPPS conditions.
26196117	14	76	used	used	2620:2623	arg2	we					2612:2613	we	2612:2613	we	2612:2613	Demonstrating the feasibility of producing homogeneously tyrosine-sulfated proteins, in addition to smaller peptides, we have used SPPS and native chemical ligation methods to synthesize the leech-derived antithrombotic protein hirudin P6, containing both tyrosine sulfation and glycosylation.
26196117	3	77	theme	sulfoforms	743:752	arg1	mixtures					731:738	heterogeneous mixtures	717:738	heterogeneous mixtures of sulfoforms	717:752	Tyrosine sulfation commonly occurs in sequences containing clusters of tyrosine residues and is incomplete at each site, resulting in heterogeneous mixtures of sulfoforms.
26196117	7	78	theme	Initial	1210:1216	arg1	studies					1228:1234	Initial synthetic studies	1210:1234	Initial synthetic studies	1210:1234	Initial synthetic studies used a cassette-based solid-phase peptide synthesis (SPPS) approach in which the side chain sulfate ester was protected to enable it to withstand Fmoc-based SPPS conditions.
26196117	10	79	from	influence	1940:1948	arg1	function					1987:1994	biochemical function	1975:1994	biochemical function	1975:1994	With homogeneously sulfated peptides in hand, we have been able to probe the influence of tyrosine sulfation on biochemical function.
26196117	9	80	theme	given	1752:1756	arg1	sequence					1758:1765	a given sequence	1750:1765	a given sequence	1750:1765	Using this methodology, we have carried out orthogonal deprotection and sulfation of up to three tyrosine residues within a given sequence, allowing access to all eight sulfoforms of a given target from a single solid-phase synthesis.
26196117	6	81	theme	functional	1163:1172	arg1	consequences					1174:1185	the functional consequences	1159:1185	the functional consequences of tyrosine sulfation	1159:1207	This Account describes our recent progress in both development of such synthetic approaches and application of the resulting sulfopeptides and sulfoproteins to characterize the functional consequences of tyrosine sulfation.
26196117	2	82	theme	chain	318:322	arg1	modification					390:401	a widespread modification	377:401	a widespread modification of extracellular and integral membrane proteins, influencing the activities of these proteins in cellular adhesion, blood clotting, inflammatory responses, and pathogen infection	377:580	Sulfation of the phenolic side chain of tyrosine residues to form sulfotyrosine (sTyr) is a widespread modification of extracellular and integral membrane proteins, influencing the activities of these proteins in cellular adhesion, blood clotting, inflammatory responses, and pathogen infection.
26196117	2	82	theme	chain	318:322	arg1	Sulfation					287:295	Sulfation	287:295	Sulfation of the phenolic side chain of tyrosine residues to form sulfotyrosine (sTyr)	287:372	Sulfation of the phenolic side chain of tyrosine residues to form sulfotyrosine (sTyr) is a widespread modification of extracellular and integral membrane proteins, influencing the activities of these proteins in cellular adhesion, blood clotting, inflammatory responses, and pathogen infection.
26196117	14	83	theme	smaller	2594:2600	arg1	peptides					2602:2609	smaller peptides	2594:2609	smaller peptides	2594:2609	Demonstrating the feasibility of producing homogeneously tyrosine-sulfated proteins, in addition to smaller peptides, we have used SPPS and native chemical ligation methods to synthesize the leech-derived antithrombotic protein hirudin P6, containing both tyrosine sulfation and glycosylation.
26196117	3	84	contain	containing	631:640	arg2	clusters					642:649	clusters	642:649	clusters of tyrosine residues	642:670	Tyrosine sulfation commonly occurs in sequences containing clusters of tyrosine residues and is incomplete at each site, resulting in heterogeneous mixtures of sulfoforms.
26196117	3	84	contain	containing	631:640	arg1	sequences					621:629	sequences	621:629	sequences containing clusters of tyrosine residues	621:670	Tyrosine sulfation commonly occurs in sequences containing clusters of tyrosine residues and is incomplete at each site, resulting in heterogeneous mixtures of sulfoforms.
26196117	2	85	theme	phenolic	304:311	arg1	chain					318:322	the phenolic side chain	300:322	the phenolic side chain of tyrosine residues to form sulfotyrosine (sTyr)	300:372	Sulfation of the phenolic side chain of tyrosine residues to form sulfotyrosine (sTyr) is a widespread modification of extracellular and integral membrane proteins, influencing the activities of these proteins in cellular adhesion, blood clotting, inflammatory responses, and pathogen infection.
26196117	14	86	theme	antithrombotic	2699:2712	arg1	P6					2730:2731	the leech-derived antithrombotic protein hirudin P6	2681:2731	the leech-derived antithrombotic protein hirudin P6	2681:2731	Demonstrating the feasibility of producing homogeneously tyrosine-sulfated proteins, in addition to smaller peptides, we have used SPPS and native chemical ligation methods to synthesize the leech-derived antithrombotic protein hirudin P6, containing both tyrosine sulfation and glycosylation.
26196117	16	87	theme	sulfopeptides	3151:3163	arg1	ligation					3129:3136	ligation	3129:3136	ligation of synthetic sulfopeptides to expressed proteins	3129:3185	In addition, the success of the ligation approach suggests that larger sulfoproteins could potentially be obtained by ligation of synthetic sulfopeptides to expressed proteins, using intein-based technology.
26196117	14	88	theme	native	2634:2639	arg1	methods					2659:2665	SPPS and native chemical ligation methods	2625:2665	SPPS and native chemical ligation methods	2625:2665	Demonstrating the feasibility of producing homogeneously tyrosine-sulfated proteins, in addition to smaller peptides, we have used SPPS and native chemical ligation methods to synthesize the leech-derived antithrombotic protein hirudin P6, containing both tyrosine sulfation and glycosylation.
26196117	0	89	theme	biochemical	136:146	arg1	function					148:155	biochemical function	136:155	biochemical function	136:155	Homogeneous sulfopeptides and sulfoproteins: synthetic approaches and applications to characterize the effects of tyrosine sulfation on biochemical function.
26196117	12	90	theme	structural	2280:2289	arg1	basis					2291:2295	the structural basis	2276:2295	the structural basis of these affinity enhancements	2276:2326	For the receptor CCR3, we showed that tyrosine sulfation enhances affinity and selectivity for binding to chemokine ligands, and we determined the structural basis of these affinity enhancements by NMR spectroscopy.
26196117	2	91	theme	tyrosine	327:334	arg1	residues					336:343	tyrosine residues	327:343	tyrosine residues	327:343	Sulfation of the phenolic side chain of tyrosine residues to form sulfotyrosine (sTyr) is a widespread modification of extracellular and integral membrane proteins, influencing the activities of these proteins in cellular adhesion, blood clotting, inflammatory responses, and pathogen infection.
26196117	5	92	theme	sulfation	888:896	arg1	influence					875:883	the influence	871:883	the influence of sulfation at each site	871:909	Therefore, the most promising approach to elucidate the influence of sulfation at each site is to prepare homogeneously sulfated proteins (or peptides) synthetically.
26196117	10	93	theme	sulfated	1882:1889	arg1	peptides					1891:1898	homogeneously sulfated peptides	1868:1898	homogeneously sulfated peptides in hand	1868:1906	With homogeneously sulfated peptides in hand, we have been able to probe the influence of tyrosine sulfation on biochemical function.
26196117	8	94	theme	divergent	1524:1532	arg1	approach					1556:1563	a divergent solid-phase synthetic approach	1522:1563	a divergent solid-phase synthetic approach utilizing orthogonally side chain protected tyrosine residues	1522:1625	Subsequently, to address the need for efficient access to multiple sulfoforms of the same peptide, we developed a divergent solid-phase synthetic approach utilizing orthogonally side chain protected tyrosine residues.
26196117	1	95	theme	Post-translational	158:175	arg1	modification					177:188	Post-translational modification	158:188	Post-translational modification of proteins	158:200	Post-translational modification of proteins plays critical roles in regulating structure, stability, localization, and function.
26196117	13	96	theme	critical	2408:2415	arg1	importance					2417:2426	the critical importance	2404:2426	the critical importance of sulfation at one specific site for supporting HIV-1 infection	2404:2491	Using a library of CCR5 sulfopeptides, we demonstrated the critical importance of sulfation at one specific site for supporting HIV-1 infection.
26196117	14	97	theme	leech-derived	2685:2697	arg1	P6					2730:2731	the leech-derived antithrombotic protein hirudin P6	2681:2731	the leech-derived antithrombotic protein hirudin P6	2681:2731	Demonstrating the feasibility of producing homogeneously tyrosine-sulfated proteins, in addition to smaller peptides, we have used SPPS and native chemical ligation methods to synthesize the leech-derived antithrombotic protein hirudin P6, containing both tyrosine sulfation and glycosylation.
26196117	8	98	theme	synthetic	1546:1554	arg1	approach					1556:1563	a divergent solid-phase synthetic approach	1522:1563	a divergent solid-phase synthetic approach utilizing orthogonally side chain protected tyrosine residues	1522:1625	Subsequently, to address the need for efficient access to multiple sulfoforms of the same peptide, we developed a divergent solid-phase synthetic approach utilizing orthogonally side chain protected tyrosine residues.
26196117	15	99	theme	sulfated	2888:2895	arg1	protein					2897:2903	the sulfated protein	2884:2903	the sulfated protein	2884:2903	Sulfation greatly enhanced inhibitory activity against thrombin, whereas addition of glycans to the sulfated protein decreased inhibition, indicating functional interplay between different post-translational modifications.
26196117	16	100	theme	larger	3075:3080	arg1	sulfoproteins					3082:3094	larger sulfoproteins	3075:3094	larger sulfoproteins	3075:3094	In addition, the success of the ligation approach suggests that larger sulfoproteins could potentially be obtained by ligation of synthetic sulfopeptides to expressed proteins, using intein-based technology.
26196117	11	101	theme	sulfated	2033:2040	arg1	fragments					2042:2050	sulfated fragments	2033:2050	sulfated fragments of chemokine receptors	2033:2073	Several of these studies focused on sulfated fragments of chemokine receptors, key mediators of leukocyte trafficking and inflammation.
26196117	11	101	theme	sulfated	2033:2040	arg1	mediators					2080:2088	key mediators	2076:2088	key mediators of leukocyte trafficking and inflammation	2076:2130	Several of these studies focused on sulfated fragments of chemokine receptors, key mediators of leukocyte trafficking and inflammation.
26196117	5	102	from	site	906:909	arg1	influence					875:883	the influence	871:883	the influence of sulfation at each site	871:909	Therefore, the most promising approach to elucidate the influence of sulfation at each site is to prepare homogeneously sulfated proteins (or peptides) synthetically.
26196117	13	103	theme	CCR5	2368:2371	arg1	sulfopeptides					2373:2385	CCR5 sulfopeptides	2368:2385	CCR5 sulfopeptides	2368:2385	Using a library of CCR5 sulfopeptides, we demonstrated the critical importance of sulfation at one specific site for supporting HIV-1 infection.
26196117	8	104	theme	tyrosine	1609:1616	arg1	residues					1618:1625	orthogonally side chain protected tyrosine residues	1575:1625	orthogonally side chain protected tyrosine residues	1575:1625	Subsequently, to address the need for efficient access to multiple sulfoforms of the same peptide, we developed a divergent solid-phase synthetic approach utilizing orthogonally side chain protected tyrosine residues.
26196117	9	105	theme	target	1819:1824	arg1	sulfoforms					1797:1806	all eight sulfoforms	1787:1806	all eight sulfoforms of a given target from a single solid-phase synthesis	1787:1860	Using this methodology, we have carried out orthogonal deprotection and sulfation of up to three tyrosine residues within a given sequence, allowing access to all eight sulfoforms of a given target from a single solid-phase synthesis.
26196117	9	106	dep	carried	1660:1666	arg1	out					1668:1670	out	1668:1670	out	1668:1670	Using this methodology, we have carried out orthogonal deprotection and sulfation of up to three tyrosine residues within a given sequence, allowing access to all eight sulfoforms of a given target from a single solid-phase synthesis.
26196117	3	107	theme	residues	663:670	arg1	clusters					642:649	clusters	642:649	clusters of tyrosine residues	642:670	Tyrosine sulfation commonly occurs in sequences containing clusters of tyrosine residues and is incomplete at each site, resulting in heterogeneous mixtures of sulfoforms.
26196117	3	108	theme	Tyrosine	583:590	arg1	sulfation					592:600	Tyrosine sulfation	583:600	Tyrosine sulfation	583:600	Tyrosine sulfation commonly occurs in sequences containing clusters of tyrosine residues and is incomplete at each site, resulting in heterogeneous mixtures of sulfoforms.
26196117	8	109	theme	side	1588:1591	arg1	residues					1618:1625	orthogonally side chain protected tyrosine residues	1575:1625	orthogonally side chain protected tyrosine residues	1575:1625	Subsequently, to address the need for efficient access to multiple sulfoforms of the same peptide, we developed a divergent solid-phase synthetic approach utilizing orthogonally side chain protected tyrosine residues.
26196117	12	110	theme	tyrosine	2171:2178	arg1	sulfation					2180:2188	tyrosine sulfation	2171:2188	tyrosine sulfation	2171:2188	For the receptor CCR3, we showed that tyrosine sulfation enhances affinity and selectivity for binding to chemokine ligands, and we determined the structural basis of these affinity enhancements by NMR spectroscopy.
26196117	7	111	theme	side	1317:1320	arg1	chain					1322:1326	the side chain	1313:1326	the side chain sulfate ester	1313:1340	Initial synthetic studies used a cassette-based solid-phase peptide synthesis (SPPS) approach in which the side chain sulfate ester was protected to enable it to withstand Fmoc-based SPPS conditions.
26196117	11	112	theme	receptors	2065:2073	arg1	fragments					2042:2050	sulfated fragments	2033:2050	sulfated fragments of chemokine receptors	2033:2073	Several of these studies focused on sulfated fragments of chemokine receptors, key mediators of leukocyte trafficking and inflammation.
26196117	11	112	theme	receptors	2065:2073	arg1	mediators					2080:2088	key mediators	2076:2088	key mediators of leukocyte trafficking and inflammation	2076:2130	Several of these studies focused on sulfated fragments of chemokine receptors, key mediators of leukocyte trafficking and inflammation.
26196117	5	113	theme	sulfated	939:946	arg1	proteins					948:955	homogeneously sulfated proteins	925:955	homogeneously sulfated proteins (or peptides)	925:969	Therefore, the most promising approach to elucidate the influence of sulfation at each site is to prepare homogeneously sulfated proteins (or peptides) synthetically.
26196117	5	113	theme	sulfated	939:946	arg1	peptides					961:968	peptides	961:968	peptides	961:968	Therefore, the most promising approach to elucidate the influence of sulfation at each site is to prepare homogeneously sulfated proteins (or peptides) synthetically.
26196117	14	114	theme	hirudin	2722:2728	arg1	P6					2730:2731	the leech-derived antithrombotic protein hirudin P6	2681:2731	the leech-derived antithrombotic protein hirudin P6	2681:2731	Demonstrating the feasibility of producing homogeneously tyrosine-sulfated proteins, in addition to smaller peptides, we have used SPPS and native chemical ligation methods to synthesize the leech-derived antithrombotic protein hirudin P6, containing both tyrosine sulfation and glycosylation.
26196117	9	115	theme	solid-phase	1840:1850	arg1	synthesis					1852:1860	a single solid-phase synthesis	1831:1860	a single solid-phase synthesis	1831:1860	Using this methodology, we have carried out orthogonal deprotection and sulfation of up to three tyrosine residues within a given sequence, allowing access to all eight sulfoforms of a given target from a single solid-phase synthesis.
26196117	8	116	theme	protected	1599:1607	arg1	residues					1618:1625	orthogonally side chain protected tyrosine residues	1575:1625	orthogonally side chain protected tyrosine residues	1575:1625	Subsequently, to address the need for efficient access to multiple sulfoforms of the same peptide, we developed a divergent solid-phase synthetic approach utilizing orthogonally side chain protected tyrosine residues.
26196117	12	117	theme	receptor	2141:2148	arg1	CCR3					2150:2153	the receptor CCR3	2137:2153	the receptor CCR3	2137:2153	For the receptor CCR3, we showed that tyrosine sulfation enhances affinity and selectivity for binding to chemokine ligands, and we determined the structural basis of these affinity enhancements by NMR spectroscopy.
26196117	6	118	theme	synthetic	1057:1065	arg1	approaches					1067:1076	such synthetic approaches	1052:1076	such synthetic approaches	1052:1076	This Account describes our recent progress in both development of such synthetic approaches and application of the resulting sulfopeptides and sulfoproteins to characterize the functional consequences of tyrosine sulfation.
26196117	15	119	theme	inhibitory	2815:2824	arg1	activity					2826:2833	inhibitory activity	2815:2833	inhibitory activity	2815:2833	Sulfation greatly enhanced inhibitory activity against thrombin, whereas addition of glycans to the sulfated protein decreased inhibition, indicating functional interplay between different post-translational modifications.
26196117	0	120	theme	sulfation	123:131	arg1	effects					103:109	the effects	99:109	the effects of tyrosine sulfation on biochemical function	99:155	Homogeneous sulfopeptides and sulfoproteins: synthetic approaches and applications to characterize the effects of tyrosine sulfation on biochemical function.
26196117	2	121	theme	extracellular	406:418	arg1	proteins					442:449	extracellular and integral membrane proteins	406:449	proteins	442:449	Sulfation of the phenolic side chain of tyrosine residues to form sulfotyrosine (sTyr) is a widespread modification of extracellular and integral membrane proteins, influencing the activities of these proteins in cellular adhesion, blood clotting, inflammatory responses, and pathogen infection.
26196117	7	122	theme	synthesis	1278:1286	arg1	approach					1295:1302	a cassette-based solid-phase peptide synthesis (SPPS) approach	1241:1302	a cassette-based solid-phase peptide synthesis (SPPS) approach in which the side chain sulfate ester was protected to enable it to withstand Fmoc-based SPPS conditions	1241:1407	Initial synthetic studies used a cassette-based solid-phase peptide synthesis (SPPS) approach in which the side chain sulfate ester was protected to enable it to withstand Fmoc-based SPPS conditions.
26196117	4	123	theme	sulfoforms	782:791	arg1	Purification					755:766	Purification	755:766	Purification of individual sulfoforms	755:791	Purification of individual sulfoforms is typically impractical.
26196117	11	124	theme	inflammation	2119:2130	arg1	fragments					2042:2050	sulfated fragments	2033:2050	sulfated fragments of chemokine receptors	2033:2073	Several of these studies focused on sulfated fragments of chemokine receptors, key mediators of leukocyte trafficking and inflammation.
26196117	11	124	theme	inflammation	2119:2130	arg1	mediators					2080:2088	key mediators	2076:2088	key mediators of leukocyte trafficking and inflammation	2076:2130	Several of these studies focused on sulfated fragments of chemokine receptors, key mediators of leukocyte trafficking and inflammation.
26196117	7	125	theme	solid-phase	1258:1268	arg1	approach					1295:1302	a cassette-based solid-phase peptide synthesis (SPPS) approach	1241:1302	a cassette-based solid-phase peptide synthesis (SPPS) approach in which the side chain sulfate ester was protected to enable it to withstand Fmoc-based SPPS conditions	1241:1407	Initial synthetic studies used a cassette-based solid-phase peptide synthesis (SPPS) approach in which the side chain sulfate ester was protected to enable it to withstand Fmoc-based SPPS conditions.
26196117	7	126	theme	SPPS	1393:1396	arg1	conditions					1398:1407	Fmoc-based SPPS conditions	1382:1407	Fmoc-based SPPS conditions	1382:1407	Initial synthetic studies used a cassette-based solid-phase peptide synthesis (SPPS) approach in which the side chain sulfate ester was protected to enable it to withstand Fmoc-based SPPS conditions.
26196117	12	127	theme	affinity	2306:2313	arg1	enhancements					2315:2326	these affinity enhancements	2300:2326	these affinity enhancements	2300:2326	For the receptor CCR3, we showed that tyrosine sulfation enhances affinity and selectivity for binding to chemokine ligands, and we determined the structural basis of these affinity enhancements by NMR spectroscopy.
26196117	2	128	theme	proteins	442:449	arg1	modification					390:401	a widespread modification	377:401	a widespread modification of extracellular and integral membrane proteins, influencing the activities of these proteins in cellular adhesion, blood clotting, inflammatory responses, and pathogen infection	377:580	Sulfation of the phenolic side chain of tyrosine residues to form sulfotyrosine (sTyr) is a widespread modification of extracellular and integral membrane proteins, influencing the activities of these proteins in cellular adhesion, blood clotting, inflammatory responses, and pathogen infection.
26196117	2	128	theme	proteins	442:449	arg1	Sulfation					287:295	Sulfation	287:295	Sulfation of the phenolic side chain of tyrosine residues to form sulfotyrosine (sTyr)	287:372	Sulfation of the phenolic side chain of tyrosine residues to form sulfotyrosine (sTyr) is a widespread modification of extracellular and integral membrane proteins, influencing the activities of these proteins in cellular adhesion, blood clotting, inflammatory responses, and pathogen infection.
26196117	0	129	theme	synthetic	45:53	arg1	approaches					55:64	synthetic approaches	45:64	synthetic approaches	45:64	Homogeneous sulfopeptides and sulfoproteins: synthetic approaches and applications to characterize the effects of tyrosine sulfation on biochemical function.
26196117	13	130	theme	HIV-1	2477:2481	arg1	infection					2483:2491	HIV-1 infection	2477:2491	HIV-1 infection	2477:2491	Using a library of CCR5 sulfopeptides, we demonstrated the critical importance of sulfation at one specific site for supporting HIV-1 infection.
26196117	6	131	theme	resulting	1101:1109	arg1	sulfopeptides					1111:1123	the resulting sulfopeptides	1097:1123	the resulting sulfopeptides	1097:1123	This Account describes our recent progress in both development of such synthetic approaches and application of the resulting sulfopeptides and sulfoproteins to characterize the functional consequences of tyrosine sulfation.
26196117	2	132	theme	integral	424:431	arg1	proteins					442:449	extracellular and integral membrane proteins	406:449	proteins	442:449	Sulfation of the phenolic side chain of tyrosine residues to form sulfotyrosine (sTyr) is a widespread modification of extracellular and integral membrane proteins, influencing the activities of these proteins in cellular adhesion, blood clotting, inflammatory responses, and pathogen infection.
26196117	7	133	used	used	1236:1239	arg2	studies					1228:1234	Initial synthetic studies	1210:1234	Initial synthetic studies	1210:1234	Initial synthetic studies used a cassette-based solid-phase peptide synthesis (SPPS) approach in which the side chain sulfate ester was protected to enable it to withstand Fmoc-based SPPS conditions.
26196117	14	134	theme	chemical	2641:2648	arg1	methods					2659:2665	SPPS and native chemical ligation methods	2625:2665	SPPS and native chemical ligation methods	2625:2665	Demonstrating the feasibility of producing homogeneously tyrosine-sulfated proteins, in addition to smaller peptides, we have used SPPS and native chemical ligation methods to synthesize the leech-derived antithrombotic protein hirudin P6, containing both tyrosine sulfation and glycosylation.
26196117	10	135	theme	biochemical	1975:1985	arg1	function					1987:1994	biochemical function	1975:1994	biochemical function	1975:1994	With homogeneously sulfated peptides in hand, we have been able to probe the influence of tyrosine sulfation on biochemical function.
26196117	2	136	dep	proteins	442:449	arg1	influencing					452:462	influencing	452:462	influencing the activities of these proteins in cellular adhesion, blood clotting, inflammatory responses, and pathogen infection	452:580	Sulfation of the phenolic side chain of tyrosine residues to form sulfotyrosine (sTyr) is a widespread modification of extracellular and integral membrane proteins, influencing the activities of these proteins in cellular adhesion, blood clotting, inflammatory responses, and pathogen infection.
26196117	14	137	theme	tyrosine-sulfated	2551:2567	arg1	proteins					2569:2576	homogeneously tyrosine-sulfated proteins	2537:2576	homogeneously tyrosine-sulfated proteins	2537:2576	Demonstrating the feasibility of producing homogeneously tyrosine-sulfated proteins, in addition to smaller peptides, we have used SPPS and native chemical ligation methods to synthesize the leech-derived antithrombotic protein hirudin P6, containing both tyrosine sulfation and glycosylation.
26196117	13	138	theme	specific	2448:2455	arg1	site					2457:2460	one specific site	2444:2460	one specific site for supporting HIV-1 infection	2444:2491	Using a library of CCR5 sulfopeptides, we demonstrated the critical importance of sulfation at one specific site for supporting HIV-1 infection.
26196117	10	139	theme	sulfation	1962:1970	arg1	influence					1940:1948	the influence	1936:1948	the influence of tyrosine sulfation on biochemical function	1936:1994	With homogeneously sulfated peptides in hand, we have been able to probe the influence of tyrosine sulfation on biochemical function.
26196117	8	140	theme	peptide	1500:1506	arg1	sulfoforms					1477:1486	multiple sulfoforms	1468:1486	multiple sulfoforms of the same peptide	1468:1506	Subsequently, to address the need for efficient access to multiple sulfoforms of the same peptide, we developed a divergent solid-phase synthetic approach utilizing orthogonally side chain protected tyrosine residues.
26196117	9	141	theme	residues	1734:1741	arg1	sulfation					1700:1708	sulfation	1700:1708	sulfation	1700:1708	Using this methodology, we have carried out orthogonal deprotection and sulfation of up to three tyrosine residues within a given sequence, allowing access to all eight sulfoforms of a given target from a single solid-phase synthesis.
26196117	9	141	theme	residues	1734:1741	arg1	deprotection					1683:1694	orthogonal deprotection	1672:1694	orthogonal deprotection	1672:1694	Using this methodology, we have carried out orthogonal deprotection and sulfation of up to three tyrosine residues within a given sequence, allowing access to all eight sulfoforms of a given target from a single solid-phase synthesis.
26196117	6	142	theme	tyrosine	1190:1197	arg1	sulfation					1199:1207	tyrosine sulfation	1190:1207	tyrosine sulfation	1190:1207	This Account describes our recent progress in both development of such synthetic approaches and application of the resulting sulfopeptides and sulfoproteins to characterize the functional consequences of tyrosine sulfation.
26196117	16	143	theme	expressed	3168:3176	arg1	proteins					3178:3185	expressed proteins	3168:3185	expressed proteins	3168:3185	In addition, the success of the ligation approach suggests that larger sulfoproteins could potentially be obtained by ligation of synthetic sulfopeptides to expressed proteins, using intein-based technology.
26871442	7	0	theme	soluble	1118:1124	arg1	CD81					1126:1129	soluble CD81	1118:1129	soluble CD81	1118:1129	The effect of these mutations on viral fitness as well as on sensitivity to inhibition by lectins, soluble CD81 or the 3/11 neutralizing antibody was assessed.
26871442	4	1	theme	HCV	636:638	arg1	resistance					640:649	the HCV resistance	632:649	the HCV resistance to CBAs	632:657	In this study we aimed at evaluating the HCV resistance to CBAs in vitro.
26871442	2	2	theme	Dengue	310:315	arg1	Virus					317:321	Dengue Virus	310:321	Dengue Virus	310:321	These molecules are able to directly inhibit many viruses such as Human Immunodeficiency Virus (HIV), Hepatitis C Virus (HCV), Dengue Virus, Ebola Virus or Severe Acute Respiratory Syndrome Coronavirus through binding to envelope protein N-glycans.
26871442	7	3	theme	neutralizing	1143:1154	arg1	antibody					1156:1163	the 3/11 neutralizing antibody	1134:1163	the 3/11 neutralizing antibody	1134:1163	The effect of these mutations on viral fitness as well as on sensitivity to inhibition by lectins, soluble CD81 or the 3/11 neutralizing antibody was assessed.
26871442	6	4	theme	strains	902:908	arg1	genome					879:884	the genome	875:884	the genome of the isolated strains	875:908	At the end of lectin exposure, the genome of the isolated strains was sequenced and several potential resistance mutations in the E1E2 envelope glycoproteins were identified.
26871442	11	5	theme	HCV	1512:1514	arg1	resistance					1516:1525	HCV resistance	1512:1525	HCV resistance to CBAs	1512:1533	Thus, our results suggest that in contrast to HIV, HCV resistance to CBAs is not directly conferred by mutations in the envelope protein genes but could occur through an indirect mechanism involving mutations in other viral proteins.
26871442	1	6	theme	Carbohydrate	61:72	arg1	agents					82:87	Carbohydrate binding agents	61:87	Carbohydrate binding agents (CBAs)	61:94	Carbohydrate binding agents (CBAs), including natural lectins, are more and more considered as broad-spectrum antivirals.
26871442	1	6	theme	Carbohydrate	61:72	arg1	antivirals					171:180	broad-spectrum antivirals	156:180	broad-spectrum antivirals	156:180	Carbohydrate binding agents (CBAs), including natural lectins, are more and more considered as broad-spectrum antivirals.
26871442	1	6	theme	Carbohydrate	61:72	arg1	lectins					115:121	natural lectins	107:121	natural lectins	107:121	Carbohydrate binding agents (CBAs), including natural lectins, are more and more considered as broad-spectrum antivirals.
26871442	1	6	theme	Carbohydrate	61:72	arg1	CBAs					90:93	CBAs	90:93	CBAs	90:93	Carbohydrate binding agents (CBAs), including natural lectins, are more and more considered as broad-spectrum antivirals.
26871442	6	7	theme	isolated	893:900	arg1	strains					902:908	the isolated strains	889:908	the isolated strains	889:908	At the end of lectin exposure, the genome of the isolated strains was sequenced and several potential resistance mutations in the E1E2 envelope glycoproteins were identified.
26871442	1	8	theme	binding	74:80	arg1	agents					82:87	Carbohydrate binding agents	61:87	Carbohydrate binding agents (CBAs)	61:94	Carbohydrate binding agents (CBAs), including natural lectins, are more and more considered as broad-spectrum antivirals.
26871442	1	8	theme	binding	74:80	arg1	antivirals					171:180	broad-spectrum antivirals	156:180	broad-spectrum antivirals	156:180	Carbohydrate binding agents (CBAs), including natural lectins, are more and more considered as broad-spectrum antivirals.
26871442	1	8	theme	binding	74:80	arg1	lectins					115:121	natural lectins	107:121	natural lectins	107:121	Carbohydrate binding agents (CBAs), including natural lectins, are more and more considered as broad-spectrum antivirals.
26871442	1	8	theme	binding	74:80	arg1	CBAs					90:93	CBAs	90:93	CBAs	90:93	Carbohydrate binding agents (CBAs), including natural lectins, are more and more considered as broad-spectrum antivirals.
26871442	11	9	theme	indirect	1631:1638	arg1	mechanism					1640:1648	an indirect mechanism	1628:1648	an indirect mechanism involving mutations in other viral proteins	1628:1692	Thus, our results suggest that in contrast to HIV, HCV resistance to CBAs is not directly conferred by mutations in the envelope protein genes but could occur through an indirect mechanism involving mutations in other viral proteins.
26871442	11	10	from	mutations	1564:1572	arg1	genes					1598:1602	the envelope protein genes	1577:1602	the envelope protein genes	1577:1602	Thus, our results suggest that in contrast to HIV, HCV resistance to CBAs is not directly conferred by mutations in the envelope protein genes but could occur through an indirect mechanism involving mutations in other viral proteins.
26871442	11	11	from	mutations	1660:1668	arg1	proteins					1685:1692	other viral proteins	1673:1692	other viral proteins	1673:1692	Thus, our results suggest that in contrast to HIV, HCV resistance to CBAs is not directly conferred by mutations in the envelope protein genes but could occur through an indirect mechanism involving mutations in other viral proteins.
26871442	5	12	theme	Cyanovirin-N	754:765	arg1	concentrations					798:811	Galanthus nivalis agglutinin (GNA), Cyanovirin-N, Concanavalin-A or Griffithsin concentrations	718:811	Galanthus nivalis agglutinin (GNA), Cyanovirin-N, Concanavalin-A or Griffithsin concentrations	718:811	HCV was cultivated in the presence of increasing Galanthus nivalis agglutinin (GNA), Cyanovirin-N, Concanavalin-A or Griffithsin concentrations, during more than eight weeks.
26871442	2	13	theme	Severe	339:344	arg1	Coronavirus					373:383	Severe Acute Respiratory Syndrome Coronavirus	339:383	Severe Acute Respiratory Syndrome Coronavirus	339:383	These molecules are able to directly inhibit many viruses such as Human Immunodeficiency Virus (HIV), Hepatitis C Virus (HCV), Dengue Virus, Ebola Virus or Severe Acute Respiratory Syndrome Coronavirus through binding to envelope protein N-glycans.
26871442	5	14	theme	nivalis	728:734	arg1	GNA					748:750	GNA	748:750	GNA	748:750	HCV was cultivated in the presence of increasing Galanthus nivalis agglutinin (GNA), Cyanovirin-N, Concanavalin-A or Griffithsin concentrations, during more than eight weeks.
26871442	5	14	theme	nivalis	728:734	arg1	agglutinin					736:745	Galanthus nivalis agglutinin	718:745	Galanthus nivalis agglutinin (GNA)	718:751	HCV was cultivated in the presence of increasing Galanthus nivalis agglutinin (GNA), Cyanovirin-N, Concanavalin-A or Griffithsin concentrations, during more than eight weeks.
26871442	5	15	theme	agglutinin	736:745	arg1	concentrations					798:811	Galanthus nivalis agglutinin (GNA), Cyanovirin-N, Concanavalin-A or Griffithsin concentrations	718:811	Galanthus nivalis agglutinin (GNA), Cyanovirin-N, Concanavalin-A or Griffithsin concentrations	718:811	HCV was cultivated in the presence of increasing Galanthus nivalis agglutinin (GNA), Cyanovirin-N, Concanavalin-A or Griffithsin concentrations, during more than eight weeks.
26871442	6	16	from	mutations	957:965	arg1	glycoproteins					988:1000	the E1E2 envelope glycoproteins	970:1000	the E1E2 envelope glycoproteins	970:1000	At the end of lectin exposure, the genome of the isolated strains was sequenced and several potential resistance mutations in the E1E2 envelope glycoproteins were identified.
26871442	2	17	theme	Immunodeficiency	255:270	arg1	HIV					279:281	HIV	279:281	HIV	279:281	These molecules are able to directly inhibit many viruses such as Human Immunodeficiency Virus (HIV), Hepatitis C Virus (HCV), Dengue Virus, Ebola Virus or Severe Acute Respiratory Syndrome Coronavirus through binding to envelope protein N-glycans.
26871442	2	17	theme	Immunodeficiency	255:270	arg1	Virus					272:276	Human Immunodeficiency Virus	249:276	Human Immunodeficiency Virus (HIV)	249:282	These molecules are able to directly inhibit many viruses such as Human Immunodeficiency Virus (HIV), Hepatitis C Virus (HCV), Dengue Virus, Ebola Virus or Severe Acute Respiratory Syndrome Coronavirus through binding to envelope protein N-glycans.
26871442	1	18	theme	broad-spectrum	156:169	arg1	antivirals					171:180	broad-spectrum antivirals	156:180	broad-spectrum antivirals	156:180	Carbohydrate binding agents (CBAs), including natural lectins, are more and more considered as broad-spectrum antivirals.
26871442	1	18	theme	broad-spectrum	156:169	arg1	agents					82:87	Carbohydrate binding agents	61:87	Carbohydrate binding agents (CBAs)	61:94	Carbohydrate binding agents (CBAs), including natural lectins, are more and more considered as broad-spectrum antivirals.
26871442	1	18	theme	broad-spectrum	156:169	arg1	lectins					115:121	natural lectins	107:121	natural lectins	107:121	Carbohydrate binding agents (CBAs), including natural lectins, are more and more considered as broad-spectrum antivirals.
26871442	0	19	theme	C	10:10	arg1	Virus					12:16	Hepatitis C Virus	0:16	Hepatitis C Virus	0:16	Hepatitis C Virus Resistance to Carbohydrate-Binding Agents.
26871442	6	20	theme	resistance	946:955	arg1	mutations					957:965	several potential resistance mutations	928:965	several potential resistance mutations in the E1E2 envelope glycoproteins	928:1000	At the end of lectin exposure, the genome of the isolated strains was sequenced and several potential resistance mutations in the E1E2 envelope glycoproteins were identified.
26871442	2	21	theme	C	295:295	arg1	HCV					304:306	HCV	304:306	HCV	304:306	These molecules are able to directly inhibit many viruses such as Human Immunodeficiency Virus (HIV), Hepatitis C Virus (HCV), Dengue Virus, Ebola Virus or Severe Acute Respiratory Syndrome Coronavirus through binding to envelope protein N-glycans.
26871442	2	21	theme	C	295:295	arg1	Virus					297:301	Hepatitis C Virus	285:301	Hepatitis C Virus (HCV)	285:307	These molecules are able to directly inhibit many viruses such as Human Immunodeficiency Virus (HIV), Hepatitis C Virus (HCV), Dengue Virus, Ebola Virus or Severe Acute Respiratory Syndrome Coronavirus through binding to envelope protein N-glycans.
26871442	6	22	theme	envelope	979:986	arg1	glycoproteins					988:1000	the E1E2 envelope glycoproteins	970:1000	the E1E2 envelope glycoproteins	970:1000	At the end of lectin exposure, the genome of the isolated strains was sequenced and several potential resistance mutations in the E1E2 envelope glycoproteins were identified.
26871442	0	23	theme	Hepatitis	0:8	arg1	Virus					12:16	Hepatitis C Virus	0:16	Hepatitis C Virus	0:16	Hepatitis C Virus Resistance to Carbohydrate-Binding Agents.
26871442	11	24	theme	other	1673:1677	arg1	proteins					1685:1692	other viral proteins	1673:1692	other viral proteins	1673:1692	Thus, our results suggest that in contrast to HIV, HCV resistance to CBAs is not directly conferred by mutations in the envelope protein genes but could occur through an indirect mechanism involving mutations in other viral proteins.
26871442	5	25	dep	increasing	707:716	arg1	the					691:693	the	691:693	the	691:693	HCV was cultivated in the presence of increasing Galanthus nivalis agglutinin (GNA), Cyanovirin-N, Concanavalin-A or Griffithsin concentrations, during more than eight weeks.
26871442	5	25	dep	increasing	707:716	arg1	presence					695:702	presence	695:702	presence	695:702	HCV was cultivated in the presence of increasing Galanthus nivalis agglutinin (GNA), Cyanovirin-N, Concanavalin-A or Griffithsin concentrations, during more than eight weeks.
26871442	2	26	theme	Hepatitis	285:293	arg1	HCV					304:306	HCV	304:306	HCV	304:306	These molecules are able to directly inhibit many viruses such as Human Immunodeficiency Virus (HIV), Hepatitis C Virus (HCV), Dengue Virus, Ebola Virus or Severe Acute Respiratory Syndrome Coronavirus through binding to envelope protein N-glycans.
26871442	2	26	theme	Hepatitis	285:293	arg1	Virus					297:301	Hepatitis C Virus	285:301	Hepatitis C Virus (HCV)	285:307	These molecules are able to directly inhibit many viruses such as Human Immunodeficiency Virus (HIV), Hepatitis C Virus (HCV), Dengue Virus, Ebola Virus or Severe Acute Respiratory Syndrome Coronavirus through binding to envelope protein N-glycans.
26871442	2	27	theme	Ebola	324:328	arg1	Virus					330:334	Ebola Virus	324:334	Ebola Virus	324:334	These molecules are able to directly inhibit many viruses such as Human Immunodeficiency Virus (HIV), Hepatitis C Virus (HCV), Dengue Virus, Ebola Virus or Severe Acute Respiratory Syndrome Coronavirus through binding to envelope protein N-glycans.
26871442	11	28	theme	viral	1679:1683	arg1	proteins					1685:1692	other viral proteins	1673:1692	other viral proteins	1673:1692	Thus, our results suggest that in contrast to HIV, HCV resistance to CBAs is not directly conferred by mutations in the envelope protein genes but could occur through an indirect mechanism involving mutations in other viral proteins.
26871442	7	29	theme	mutations	1039:1047	arg1	effect					1023:1028	The effect	1019:1028	The effect of these mutations on viral fitness as well as on sensitivity to inhibition by lectins, soluble CD81 or the 3/11 neutralizing antibody	1019:1163	The effect of these mutations on viral fitness as well as on sensitivity to inhibition by lectins, soluble CD81 or the 3/11 neutralizing antibody was assessed.
26871442	9	30	theme	CD81-LEL	1347:1354	arg1	inhibition					1356:1365	CD81-LEL inhibition	1347:1365	CD81-LEL inhibition	1347:1365	In contrast, we observed that some mutants were more sensitive to 3/11 or CD81-LEL inhibition.
26871442	10	31	theme	several	1382:1388	arg1	mutations					1390:1398	several mutations	1382:1398	several mutations	1382:1398	Additionally, several mutations were identified in the Core and the non-structural proteins.
26871442	3	32	theme	mutant	491:496	arg1	viruses					498:504	mutant viruses	491:504	mutant viruses	491:504	In the case of HIV, it has been shown that CBAs select for mutant viruses with N-glycosylation site deletions which are more sensitive to neutralizing antibodies.
26871442	2	33	theme	Syndrome	364:371	arg1	Coronavirus					373:383	Severe Acute Respiratory Syndrome Coronavirus	339:383	Severe Acute Respiratory Syndrome Coronavirus	339:383	These molecules are able to directly inhibit many viruses such as Human Immunodeficiency Virus (HIV), Hepatitis C Virus (HCV), Dengue Virus, Ebola Virus or Severe Acute Respiratory Syndrome Coronavirus through binding to envelope protein N-glycans.
26871442	0	34	theme	Carbohydrate-Binding	32:51	arg1	Agents					53:58	Carbohydrate-Binding Agents	32:58	Carbohydrate-Binding Agents	32:58	Hepatitis C Virus Resistance to Carbohydrate-Binding Agents.
26871442	7	35	from	effect	1023:1028	arg1	sensitivity					1080:1090	sensitivity	1080:1090	sensitivity to inhibition by lectins, soluble CD81 or the 3/11 neutralizing antibody	1080:1163	The effect of these mutations on viral fitness as well as on sensitivity to inhibition by lectins, soluble CD81 or the 3/11 neutralizing antibody was assessed.
26871442	7	35	from	effect	1023:1028	arg1	fitness					1058:1064	viral fitness	1052:1064	viral fitness	1052:1064	The effect of these mutations on viral fitness as well as on sensitivity to inhibition by lectins, soluble CD81 or the 3/11 neutralizing antibody was assessed.
26871442	2	36	theme	many	228:231	arg1	Virus					317:321	Dengue Virus	310:321	Dengue Virus	310:321	These molecules are able to directly inhibit many viruses such as Human Immunodeficiency Virus (HIV), Hepatitis C Virus (HCV), Dengue Virus, Ebola Virus or Severe Acute Respiratory Syndrome Coronavirus through binding to envelope protein N-glycans.
26871442	2	36	theme	many	228:231	arg1	Virus					272:276	Human Immunodeficiency Virus	249:276	Human Immunodeficiency Virus (HIV)	249:282	These molecules are able to directly inhibit many viruses such as Human Immunodeficiency Virus (HIV), Hepatitis C Virus (HCV), Dengue Virus, Ebola Virus or Severe Acute Respiratory Syndrome Coronavirus through binding to envelope protein N-glycans.
26871442	2	36	theme	many	228:231	arg1	Virus					297:301	Hepatitis C Virus	285:301	Hepatitis C Virus (HCV)	285:307	These molecules are able to directly inhibit many viruses such as Human Immunodeficiency Virus (HIV), Hepatitis C Virus (HCV), Dengue Virus, Ebola Virus or Severe Acute Respiratory Syndrome Coronavirus through binding to envelope protein N-glycans.
26871442	2	36	theme	many	228:231	arg1	viruses					233:239	many viruses	228:239	many viruses such as Human Immunodeficiency Virus (HIV), Hepatitis C Virus (HCV), Dengue Virus, Ebola Virus or Severe Acute Respiratory Syndrome Coronavirus	228:383	These molecules are able to directly inhibit many viruses such as Human Immunodeficiency Virus (HIV), Hepatitis C Virus (HCV), Dengue Virus, Ebola Virus or Severe Acute Respiratory Syndrome Coronavirus through binding to envelope protein N-glycans.
26871442	2	36	theme	many	228:231	arg1	Coronavirus					373:383	Severe Acute Respiratory Syndrome Coronavirus	339:383	Severe Acute Respiratory Syndrome Coronavirus	339:383	These molecules are able to directly inhibit many viruses such as Human Immunodeficiency Virus (HIV), Hepatitis C Virus (HCV), Dengue Virus, Ebola Virus or Severe Acute Respiratory Syndrome Coronavirus through binding to envelope protein N-glycans.
26871442	2	36	theme	many	228:231	arg1	Virus					330:334	Ebola Virus	324:334	Ebola Virus	324:334	These molecules are able to directly inhibit many viruses such as Human Immunodeficiency Virus (HIV), Hepatitis C Virus (HCV), Dengue Virus, Ebola Virus or Severe Acute Respiratory Syndrome Coronavirus through binding to envelope protein N-glycans.
26871442	6	37	theme	E1E2	974:977	arg1	glycoproteins					988:1000	the E1E2 envelope glycoproteins	970:1000	the E1E2 envelope glycoproteins	970:1000	At the end of lectin exposure, the genome of the isolated strains was sequenced and several potential resistance mutations in the E1E2 envelope glycoproteins were identified.
26871442	2	38	theme	Respiratory	352:362	arg1	Coronavirus					373:383	Severe Acute Respiratory Syndrome Coronavirus	339:383	Severe Acute Respiratory Syndrome Coronavirus	339:383	These molecules are able to directly inhibit many viruses such as Human Immunodeficiency Virus (HIV), Hepatitis C Virus (HCV), Dengue Virus, Ebola Virus or Severe Acute Respiratory Syndrome Coronavirus through binding to envelope protein N-glycans.
26871442	6	39	theme	exposure	865:872	arg1	end					851:853	the end	847:853	the end of lectin exposure	847:872	At the end of lectin exposure, the genome of the isolated strains was sequenced and several potential resistance mutations in the E1E2 envelope glycoproteins were identified.
26871442	12	40	theme	underlying	1757:1766	arg1	mechanisms					1768:1777	the underlying mechanisms	1753:1777	the underlying mechanisms	1753:1777	Further investigations are needed to completely elucidate the underlying mechanisms.
26871442	3	41	theme	neutralizing	570:581	arg1	antibodies					583:592	neutralizing antibodies	570:592	neutralizing antibodies	570:592	In the case of HIV, it has been shown that CBAs select for mutant viruses with N-glycosylation site deletions which are more sensitive to neutralizing antibodies.
26871442	5	42	theme	Concanavalin-A	768:781	arg1	concentrations					798:811	Galanthus nivalis agglutinin (GNA), Cyanovirin-N, Concanavalin-A or Griffithsin concentrations	718:811	Galanthus nivalis agglutinin (GNA), Cyanovirin-N, Concanavalin-A or Griffithsin concentrations	718:811	HCV was cultivated in the presence of increasing Galanthus nivalis agglutinin (GNA), Cyanovirin-N, Concanavalin-A or Griffithsin concentrations, during more than eight weeks.
26871442	3	43	gly	N-glycosylation	511:525	arg2	site					527:530	N-glycosylation site deletions	511:540	N-glycosylation site deletions which are more sensitive to neutralizing antibodies	511:592	In the case of HIV, it has been shown that CBAs select for mutant viruses with N-glycosylation site deletions which are more sensitive to neutralizing antibodies.
26871442	3	43	gly	N-glycosylation	511:525	arg2	deletions					532:540	N-glycosylation site deletions	511:540	N-glycosylation site deletions which are more sensitive to neutralizing antibodies	511:592	In the case of HIV, it has been shown that CBAs select for mutant viruses with N-glycosylation site deletions which are more sensitive to neutralizing antibodies.
26871442	5	44	theme	Galanthus	718:726	arg1	GNA					748:750	GNA	748:750	GNA	748:750	HCV was cultivated in the presence of increasing Galanthus nivalis agglutinin (GNA), Cyanovirin-N, Concanavalin-A or Griffithsin concentrations, during more than eight weeks.
26871442	5	44	theme	Galanthus	718:726	arg1	agglutinin					736:745	Galanthus nivalis agglutinin	718:745	Galanthus nivalis agglutinin (GNA)	718:751	HCV was cultivated in the presence of increasing Galanthus nivalis agglutinin (GNA), Cyanovirin-N, Concanavalin-A or Griffithsin concentrations, during more than eight weeks.
26871442	6	45	theme	lectin	858:863	arg1	exposure					865:872	lectin exposure	858:872	lectin exposure	858:872	At the end of lectin exposure, the genome of the isolated strains was sequenced and several potential resistance mutations in the E1E2 envelope glycoproteins were identified.
26871442	6	46	gly	glycoproteins	988:1000	arg1	glycoproteins					988:1000	the E1E2 envelope glycoproteins	970:1000	the E1E2 envelope glycoproteins	970:1000	At the end of lectin exposure, the genome of the isolated strains was sequenced and several potential resistance mutations in the E1E2 envelope glycoproteins were identified.
26871442	3	47	theme	N-glycosylation	511:525	arg1	deletions					532:540	N-glycosylation site deletions	511:540	N-glycosylation site deletions which are more sensitive to neutralizing antibodies	511:592	In the case of HIV, it has been shown that CBAs select for mutant viruses with N-glycosylation site deletions which are more sensitive to neutralizing antibodies.
26871442	1	48	theme	natural	107:113	arg1	lectins					115:121	natural lectins	107:121	natural lectins	107:121	Carbohydrate binding agents (CBAs), including natural lectins, are more and more considered as broad-spectrum antivirals.
26871442	2	49	theme	protein	413:419	arg1	N-glycans					421:429	envelope protein N-glycans	404:429	envelope protein N-glycans	404:429	These molecules are able to directly inhibit many viruses such as Human Immunodeficiency Virus (HIV), Hepatitis C Virus (HCV), Dengue Virus, Ebola Virus or Severe Acute Respiratory Syndrome Coronavirus through binding to envelope protein N-glycans.
26871442	11	50	theme	protein	1590:1596	arg1	genes					1598:1602	the envelope protein genes	1577:1602	the envelope protein genes	1577:1602	Thus, our results suggest that in contrast to HIV, HCV resistance to CBAs is not directly conferred by mutations in the envelope protein genes but could occur through an indirect mechanism involving mutations in other viral proteins.
26871442	3	51	theme	site	527:530	arg1	deletions					532:540	N-glycosylation site deletions	511:540	N-glycosylation site deletions which are more sensitive to neutralizing antibodies	511:592	In the case of HIV, it has been shown that CBAs select for mutant viruses with N-glycosylation site deletions which are more sensitive to neutralizing antibodies.
26871442	3	52	theme	HIV	447:449	arg1	case					439:442	the case	435:442	the case of HIV	435:449	In the case of HIV, it has been shown that CBAs select for mutant viruses with N-glycosylation site deletions which are more sensitive to neutralizing antibodies.
26871442	2	53	theme	Human	249:253	arg1	HIV					279:281	HIV	279:281	HIV	279:281	These molecules are able to directly inhibit many viruses such as Human Immunodeficiency Virus (HIV), Hepatitis C Virus (HCV), Dengue Virus, Ebola Virus or Severe Acute Respiratory Syndrome Coronavirus through binding to envelope protein N-glycans.
26871442	2	53	theme	Human	249:253	arg1	Virus					272:276	Human Immunodeficiency Virus	249:276	Human Immunodeficiency Virus (HIV)	249:282	These molecules are able to directly inhibit many viruses such as Human Immunodeficiency Virus (HIV), Hepatitis C Virus (HCV), Dengue Virus, Ebola Virus or Severe Acute Respiratory Syndrome Coronavirus through binding to envelope protein N-glycans.
26871442	11	54	theme	envelope	1581:1588	arg1	genes					1598:1602	the envelope protein genes	1577:1602	the envelope protein genes	1577:1602	Thus, our results suggest that in contrast to HIV, HCV resistance to CBAs is not directly conferred by mutations in the envelope protein genes but could occur through an indirect mechanism involving mutations in other viral proteins.
26871442	8	55	theme	mutations	1207:1215	arg1	none					1193:1196	none	1193:1196	none	1193:1196	Surprisingly, none of these mutations, alone or in combination, conferred resistance to CBAs.
26871442	6	56	theme	potential	936:944	arg1	mutations					957:965	several potential resistance mutations	928:965	several potential resistance mutations in the E1E2 envelope glycoproteins	928:1000	At the end of lectin exposure, the genome of the isolated strains was sequenced and several potential resistance mutations in the E1E2 envelope glycoproteins were identified.
26871442	12	57	theme	Further	1695:1701	arg1	investigations					1703:1716	Further investigations	1695:1716	Further investigations	1695:1716	Further investigations are needed to completely elucidate the underlying mechanisms.
26871442	6	58	theme	several	928:934	arg1	mutations					957:965	several potential resistance mutations	928:965	several potential resistance mutations in the E1E2 envelope glycoproteins	928:1000	At the end of lectin exposure, the genome of the isolated strains was sequenced and several potential resistance mutations in the E1E2 envelope glycoproteins were identified.
26871442	10	59	theme	non-structural	1436:1449	arg1	proteins					1451:1458	the non-structural proteins	1432:1458	the non-structural proteins	1432:1458	Additionally, several mutations were identified in the Core and the non-structural proteins.
26871442	2	60	theme	Acute	346:350	arg1	Coronavirus					373:383	Severe Acute Respiratory Syndrome Coronavirus	339:383	Severe Acute Respiratory Syndrome Coronavirus	339:383	These molecules are able to directly inhibit many viruses such as Human Immunodeficiency Virus (HIV), Hepatitis C Virus (HCV), Dengue Virus, Ebola Virus or Severe Acute Respiratory Syndrome Coronavirus through binding to envelope protein N-glycans.
26871442	5	61	theme	Griffithsin	786:796	arg1	concentrations					798:811	Galanthus nivalis agglutinin (GNA), Cyanovirin-N, Concanavalin-A or Griffithsin concentrations	718:811	Galanthus nivalis agglutinin (GNA), Cyanovirin-N, Concanavalin-A or Griffithsin concentrations	718:811	HCV was cultivated in the presence of increasing Galanthus nivalis agglutinin (GNA), Cyanovirin-N, Concanavalin-A or Griffithsin concentrations, during more than eight weeks.
26871442	7	62	theme	viral	1052:1056	arg1	fitness					1058:1064	viral fitness	1052:1064	viral fitness	1052:1064	The effect of these mutations on viral fitness as well as on sensitivity to inhibition by lectins, soluble CD81 or the 3/11 neutralizing antibody was assessed.
26871442	2	63	theme	envelope	404:411	arg1	N-glycans					421:429	envelope protein N-glycans	404:429	envelope protein N-glycans	404:429	These molecules are able to directly inhibit many viruses such as Human Immunodeficiency Virus (HIV), Hepatitis C Virus (HCV), Dengue Virus, Ebola Virus or Severe Acute Respiratory Syndrome Coronavirus through binding to envelope protein N-glycans.
26871442	7	64	theme	3/11	1138:1141	arg1	antibody					1156:1163	the 3/11 neutralizing antibody	1134:1163	the 3/11 neutralizing antibody	1134:1163	The effect of these mutations on viral fitness as well as on sensitivity to inhibition by lectins, soluble CD81 or the 3/11 neutralizing antibody was assessed.
25770467	3	0	theme	emission	571:578	arg1	depletion					595:603	the carbon dioxide emission and fossil oil depletion	552:603	the carbon dioxide emission and fossil oil depletion	552:603	The comparative life cycle assessment results show that the carbon dioxide emission and fossil oil depletion could be reduced by 95% and 23% respectively in case of standalone bioethanol system, and 99% and 17% respectively in case of bioethanol and furfural system compared to that of conventional petrol and furfural systems.
25770467	4	1	theme	energy	939:944	arg1	source					929:934	a source	927:934	a source of energy	927:944	The sensitivity study indicates that the impact could be further reduced if vetiver biomass is used as a source of energy in biorefinery plant instead to the coal.
25770467	4	1	theme	energy	939:944	arg1	biomass					908:914	vetiver biomass	900:914	vetiver biomass	900:914	The sensitivity study indicates that the impact could be further reduced if vetiver biomass is used as a source of energy in biorefinery plant instead to the coal.
25770467	3	2	theme	carbon	556:561	arg1	emission					571:578	carbon dioxide emission	556:578	carbon dioxide emission	556:578	The comparative life cycle assessment results show that the carbon dioxide emission and fossil oil depletion could be reduced by 95% and 23% respectively in case of standalone bioethanol system, and 99% and 17% respectively in case of bioethanol and furfural system compared to that of conventional petrol and furfural systems.
25770467	3	3	dep	bioethanol	731:740	arg1	system					755:760	system	755:760	system	755:760	The comparative life cycle assessment results show that the carbon dioxide emission and fossil oil depletion could be reduced by 95% and 23% respectively in case of standalone bioethanol system, and 99% and 17% respectively in case of bioethanol and furfural system compared to that of conventional petrol and furfural systems.
25770467	1	4	dep	vetiver	133:139	arg1	leaves					141:146	leaves	141:146	leaves	141:146	In this study a prospective life cycle assessment of biorefinery system from vetiver leaves was carried out to know the environmental benefits of this system over conventional systems considering the geographical context of India.
25770467	4	5	theme	sensitivity	828:838	arg1	study					840:844	The sensitivity study	824:844	The sensitivity study	824:844	The sensitivity study indicates that the impact could be further reduced if vetiver biomass is used as a source of energy in biorefinery plant instead to the coal.
25770467	0	6	from	vetiver	47:53	arg1	LCA					0:2	LCA	0:2	LCA of bioethanol and furfural production from vetiver.	0:54	LCA of bioethanol and furfural production from vetiver.
25770467	1	7	theme	geographical	256:267	arg1	context					269:275	the geographical context	252:275	the geographical context of India	252:284	In this study a prospective life cycle assessment of biorefinery system from vetiver leaves was carried out to know the environmental benefits of this system over conventional systems considering the geographical context of India.
25770467	1	8	from	vetiver	133:139	arg1	assessment					95:104	a prospective life cycle assessment	70:104	a prospective life cycle assessment of biorefinery system from vetiver leaves	70:146	In this study a prospective life cycle assessment of biorefinery system from vetiver leaves was carried out to know the environmental benefits of this system over conventional systems considering the geographical context of India.
25770467	2	9	from	lignin	430:435	arg1	rich					377:380	rich	377:380	rich	377:380	The composition of vetiver leaves from the experimental analysis revealed that vetiver is rich in cellulose (32.6%), hemicellulose (31.5%) and lignin (17.3%) that could be used as a feedstock for biorefinery.
25770467	3	10	dep	emission	571:578	arg1	the					552:554	the	552:554	the	552:554	The comparative life cycle assessment results show that the carbon dioxide emission and fossil oil depletion could be reduced by 95% and 23% respectively in case of standalone bioethanol system, and 99% and 17% respectively in case of bioethanol and furfural system compared to that of conventional petrol and furfural systems.
25770467	2	11	dep	vetiver	306:312	arg1	leaves					314:319	leaves	314:319	leaves from the experimental analysis	314:350	The composition of vetiver leaves from the experimental analysis revealed that vetiver is rich in cellulose (32.6%), hemicellulose (31.5%) and lignin (17.3%) that could be used as a feedstock for biorefinery.
25770467	2	12	theme	vetiver	306:312	arg1	composition					291:301	The composition	287:301	The composition of vetiver leaves from the experimental analysis	287:350	The composition of vetiver leaves from the experimental analysis revealed that vetiver is rich in cellulose (32.6%), hemicellulose (31.5%) and lignin (17.3%) that could be used as a feedstock for biorefinery.
25770467	3	13	theme	conventional	782:793	arg1	petrol					795:800	conventional petrol and furfural systems	782:821	petrol	795:800	The comparative life cycle assessment results show that the carbon dioxide emission and fossil oil depletion could be reduced by 95% and 23% respectively in case of standalone bioethanol system, and 99% and 17% respectively in case of bioethanol and furfural system compared to that of conventional petrol and furfural systems.
25770467	1	14	theme	prospective	72:82	arg1	assessment					95:104	a prospective life cycle assessment	70:104	a prospective life cycle assessment of biorefinery system from vetiver leaves	70:146	In this study a prospective life cycle assessment of biorefinery system from vetiver leaves was carried out to know the environmental benefits of this system over conventional systems considering the geographical context of India.
25770467	3	15	theme	standalone	661:670	arg1	system					683:688	standalone bioethanol system	661:688	standalone bioethanol system	661:688	The comparative life cycle assessment results show that the carbon dioxide emission and fossil oil depletion could be reduced by 95% and 23% respectively in case of standalone bioethanol system, and 99% and 17% respectively in case of bioethanol and furfural system compared to that of conventional petrol and furfural systems.
25770467	1	16	theme	life	84:87	arg1	assessment					95:104	a prospective life cycle assessment	70:104	a prospective life cycle assessment of biorefinery system from vetiver leaves	70:146	In this study a prospective life cycle assessment of biorefinery system from vetiver leaves was carried out to know the environmental benefits of this system over conventional systems considering the geographical context of India.
25770467	0	17	theme	bioethanol	7:16	arg1	LCA					0:2	LCA	0:2	LCA of bioethanol and furfural production from vetiver.	0:54	LCA of bioethanol and furfural production from vetiver.
25770467	1	18	theme	India	280:284	arg1	context					269:275	the geographical context	252:275	the geographical context of India	252:284	In this study a prospective life cycle assessment of biorefinery system from vetiver leaves was carried out to know the environmental benefits of this system over conventional systems considering the geographical context of India.
25770467	1	19	theme	cycle	89:93	arg1	assessment					95:104	a prospective life cycle assessment	70:104	a prospective life cycle assessment of biorefinery system from vetiver leaves	70:146	In this study a prospective life cycle assessment of biorefinery system from vetiver leaves was carried out to know the environmental benefits of this system over conventional systems considering the geographical context of India.
25770467	3	20	theme	furfural	806:813	arg1	systems					815:821	conventional petrol and furfural systems	782:821	systems	815:821	The comparative life cycle assessment results show that the carbon dioxide emission and fossil oil depletion could be reduced by 95% and 23% respectively in case of standalone bioethanol system, and 99% and 17% respectively in case of bioethanol and furfural system compared to that of conventional petrol and furfural systems.
25770467	1	21	theme	environmental	176:188	arg1	benefits					190:197	the environmental benefits	172:197	the environmental benefits of this system over conventional systems considering the geographical context of India	172:284	In this study a prospective life cycle assessment of biorefinery system from vetiver leaves was carried out to know the environmental benefits of this system over conventional systems considering the geographical context of India.
25770467	3	22	theme	fossil	584:589	arg1	oil					591:593	fossil oil	584:593	fossil oil	584:593	The comparative life cycle assessment results show that the carbon dioxide emission and fossil oil depletion could be reduced by 95% and 23% respectively in case of standalone bioethanol system, and 99% and 17% respectively in case of bioethanol and furfural system compared to that of conventional petrol and furfural systems.
25770467	3	23	theme	assessment	523:532	arg1	results					534:540	The comparative life cycle assessment results	496:540	The comparative life cycle assessment results	496:540	The comparative life cycle assessment results show that the carbon dioxide emission and fossil oil depletion could be reduced by 95% and 23% respectively in case of standalone bioethanol system, and 99% and 17% respectively in case of bioethanol and furfural system compared to that of conventional petrol and furfural systems.
25770467	0	24	theme	furfural	22:29	arg1	LCA					0:2	LCA	0:2	LCA of bioethanol and furfural production from vetiver.	0:54	LCA of bioethanol and furfural production from vetiver.
25770467	3	25	theme	dioxide	563:569	arg1	emission					571:578	carbon dioxide emission	556:578	carbon dioxide emission	556:578	The comparative life cycle assessment results show that the carbon dioxide emission and fossil oil depletion could be reduced by 95% and 23% respectively in case of standalone bioethanol system, and 99% and 17% respectively in case of bioethanol and furfural system compared to that of conventional petrol and furfural systems.
25770467	2	26	from	rich	377:380	arg1	cellulose					385:393	cellulose	385:393	cellulose (32.6%)	385:401	The composition of vetiver leaves from the experimental analysis revealed that vetiver is rich in cellulose (32.6%), hemicellulose (31.5%) and lignin (17.3%) that could be used as a feedstock for biorefinery.
25770467	2	26	from	rich	377:380	arg1	hemicellulose					404:416	hemicellulose	404:416	hemicellulose (31.5%)	404:424	The composition of vetiver leaves from the experimental analysis revealed that vetiver is rich in cellulose (32.6%), hemicellulose (31.5%) and lignin (17.3%) that could be used as a feedstock for biorefinery.
25770467	2	26	from	rich	377:380	arg1	%					442:442	17.3%	438:442	17.3%	438:442	The composition of vetiver leaves from the experimental analysis revealed that vetiver is rich in cellulose (32.6%), hemicellulose (31.5%) and lignin (17.3%) that could be used as a feedstock for biorefinery.
25770467	2	26	from	rich	377:380	arg1	%					400:400	32.6%	396:400	32.6%	396:400	The composition of vetiver leaves from the experimental analysis revealed that vetiver is rich in cellulose (32.6%), hemicellulose (31.5%) and lignin (17.3%) that could be used as a feedstock for biorefinery.
25770467	2	26	from	rich	377:380	arg1	%					423:423	31.5%	419:423	31.5%	419:423	The composition of vetiver leaves from the experimental analysis revealed that vetiver is rich in cellulose (32.6%), hemicellulose (31.5%) and lignin (17.3%) that could be used as a feedstock for biorefinery.
25770467	2	26	from	rich	377:380	arg1	feedstock					469:477	a feedstock	467:477	a feedstock for biorefinery	467:493	The composition of vetiver leaves from the experimental analysis revealed that vetiver is rich in cellulose (32.6%), hemicellulose (31.5%) and lignin (17.3%) that could be used as a feedstock for biorefinery.
25770467	2	26	from	rich	377:380	arg1	lignin					430:435	lignin	430:435	lignin (17.3%) that could be used as a feedstock for biorefinery	430:493	The composition of vetiver leaves from the experimental analysis revealed that vetiver is rich in cellulose (32.6%), hemicellulose (31.5%) and lignin (17.3%) that could be used as a feedstock for biorefinery.
25770467	4	27	theme	vetiver	900:906	arg1	source					929:934	a source	927:934	a source of energy	927:944	The sensitivity study indicates that the impact could be further reduced if vetiver biomass is used as a source of energy in biorefinery plant instead to the coal.
25770467	4	27	theme	vetiver	900:906	arg1	biomass					908:914	vetiver biomass	900:914	vetiver biomass	900:914	The sensitivity study indicates that the impact could be further reduced if vetiver biomass is used as a source of energy in biorefinery plant instead to the coal.
25770467	2	28	used	used	459:462	arg2	lignin					430:435	lignin	430:435	lignin (17.3%) that could be used as a feedstock for biorefinery	430:493	The composition of vetiver leaves from the experimental analysis revealed that vetiver is rich in cellulose (32.6%), hemicellulose (31.5%) and lignin (17.3%) that could be used as a feedstock for biorefinery.
25770467	2	28	used	used	459:462	arg2	%					442:442	17.3%	438:442	17.3%	438:442	The composition of vetiver leaves from the experimental analysis revealed that vetiver is rich in cellulose (32.6%), hemicellulose (31.5%) and lignin (17.3%) that could be used as a feedstock for biorefinery.
25770467	2	28	used	used	459:462	arg2	feedstock					469:477	a feedstock	467:477	a feedstock for biorefinery	467:493	The composition of vetiver leaves from the experimental analysis revealed that vetiver is rich in cellulose (32.6%), hemicellulose (31.5%) and lignin (17.3%) that could be used as a feedstock for biorefinery.
25770467	3	29	theme	life	512:515	arg1	assessment					523:532	The comparative life cycle assessment	496:532	The comparative life cycle assessment results	496:540	The comparative life cycle assessment results show that the carbon dioxide emission and fossil oil depletion could be reduced by 95% and 23% respectively in case of standalone bioethanol system, and 99% and 17% respectively in case of bioethanol and furfural system compared to that of conventional petrol and furfural systems.
25770467	2	30	theme	experimental	330:341	arg1	analysis					343:350	the experimental analysis	326:350	the experimental analysis	326:350	The composition of vetiver leaves from the experimental analysis revealed that vetiver is rich in cellulose (32.6%), hemicellulose (31.5%) and lignin (17.3%) that could be used as a feedstock for biorefinery.
25770467	3	31	theme	bioethanol	672:681	arg1	system					683:688	standalone bioethanol system	661:688	standalone bioethanol system	661:688	The comparative life cycle assessment results show that the carbon dioxide emission and fossil oil depletion could be reduced by 95% and 23% respectively in case of standalone bioethanol system, and 99% and 17% respectively in case of bioethanol and furfural system compared to that of conventional petrol and furfural systems.
25770467	4	32	used	used	919:922	arg2	biomass					908:914	vetiver biomass	900:914	vetiver biomass	900:914	The sensitivity study indicates that the impact could be further reduced if vetiver biomass is used as a source of energy in biorefinery plant instead to the coal.
25770467	4	32	used	used	919:922	arg2	source					929:934	a source	927:934	a source of energy	927:944	The sensitivity study indicates that the impact could be further reduced if vetiver biomass is used as a source of energy in biorefinery plant instead to the coal.
25770467	3	33	theme	cycle	517:521	arg1	assessment					523:532	The comparative life cycle assessment	496:532	The comparative life cycle assessment results	496:540	The comparative life cycle assessment results show that the carbon dioxide emission and fossil oil depletion could be reduced by 95% and 23% respectively in case of standalone bioethanol system, and 99% and 17% respectively in case of bioethanol and furfural system compared to that of conventional petrol and furfural systems.
25770467	2	34	from	cellulose	385:393	arg1	rich					377:380	rich	377:380	rich	377:380	The composition of vetiver leaves from the experimental analysis revealed that vetiver is rich in cellulose (32.6%), hemicellulose (31.5%) and lignin (17.3%) that could be used as a feedstock for biorefinery.
25770467	4	35	theme	biorefinery	949:959	arg1	plant					961:965	biorefinery plant	949:965	biorefinery plant instead to the coal	949:985	The sensitivity study indicates that the impact could be further reduced if vetiver biomass is used as a source of energy in biorefinery plant instead to the coal.
25770467	1	36	theme	biorefinery	109:119	arg1	system					121:126	biorefinery system	109:126	biorefinery system	109:126	In this study a prospective life cycle assessment of biorefinery system from vetiver leaves was carried out to know the environmental benefits of this system over conventional systems considering the geographical context of India.
25770467	3	37	theme	oil	591:593	arg1	depletion					595:603	the carbon dioxide emission and fossil oil depletion	552:603	the carbon dioxide emission and fossil oil depletion	552:603	The comparative life cycle assessment results show that the carbon dioxide emission and fossil oil depletion could be reduced by 95% and 23% respectively in case of standalone bioethanol system, and 99% and 17% respectively in case of bioethanol and furfural system compared to that of conventional petrol and furfural systems.
25770467	0	38	dep	bioethanol	7:16	arg1	production					31:40	production	31:40	production	31:40	LCA of bioethanol and furfural production from vetiver.
25770467	1	39	theme	system	121:126	arg1	assessment					95:104	a prospective life cycle assessment	70:104	a prospective life cycle assessment of biorefinery system from vetiver leaves	70:146	In this study a prospective life cycle assessment of biorefinery system from vetiver leaves was carried out to know the environmental benefits of this system over conventional systems considering the geographical context of India.
25770467	1	40	theme	system	207:212	arg1	benefits					190:197	the environmental benefits	172:197	the environmental benefits of this system over conventional systems considering the geographical context of India	172:284	In this study a prospective life cycle assessment of biorefinery system from vetiver leaves was carried out to know the environmental benefits of this system over conventional systems considering the geographical context of India.
25770467	2	41	from	hemicellulose	404:416	arg1	rich					377:380	rich	377:380	rich	377:380	The composition of vetiver leaves from the experimental analysis revealed that vetiver is rich in cellulose (32.6%), hemicellulose (31.5%) and lignin (17.3%) that could be used as a feedstock for biorefinery.
25770467	3	42	theme	comparative	500:510	arg1	assessment					523:532	The comparative life cycle assessment	496:532	The comparative life cycle assessment results	496:540	The comparative life cycle assessment results show that the carbon dioxide emission and fossil oil depletion could be reduced by 95% and 23% respectively in case of standalone bioethanol system, and 99% and 17% respectively in case of bioethanol and furfural system compared to that of conventional petrol and furfural systems.
25770467	1	43	theme	conventional	219:230	arg1	systems					232:238	conventional systems	219:238	conventional systems considering the geographical context of India	219:284	In this study a prospective life cycle assessment of biorefinery system from vetiver leaves was carried out to know the environmental benefits of this system over conventional systems considering the geographical context of India.
25665785	1	0	theme	composition	281:291	arg1	analysis					293:300	composition analysis	281:300	composition analysis	281:300	O-Specific polysaccharides were obtained from the lipopolysaccharides isolated from the planktonic and biofilm cultures of Pseudomonas chlororaphis 449 and studied by composition analysis and 1D and 2D (1)H and (13)C NMR spectroscopy.
25665785	1	1	theme	C	329:329	arg1	spectroscopy					335:346	(13)C NMR spectroscopy	325:346	(13)C NMR spectroscopy	325:346	O-Specific polysaccharides were obtained from the lipopolysaccharides isolated from the planktonic and biofilm cultures of Pseudomonas chlororaphis 449 and studied by composition analysis and 1D and 2D (1)H and (13)C NMR spectroscopy.
25665785	2	2	theme	planktonic	557:566	arg1	form					568:571	the planktonic form	553:571	the planktonic form	553:571	The following structure was established: -->4)-α-D-GalpNAc6Ac-(1-->3)-β-D-QuipNAc-(1-->6)-α-D-GlcpNAc-(1-->β-D-GlcpNAc-(1-->3) where the degree of non-stoichiometric 6-O-acetylation of GalNAc is ∼ 60% in the planktonic form or ∼ 10% in biofilm.
25665785	0	3	theme	449	109:111	arg1	cultures					72:79	planktonic and biofilm cultures	49:79	planktonic and biofilm cultures of Pseudomonas chlororaphis 449	49:111	Structure of the O-specific polysaccharides from planktonic and biofilm cultures of Pseudomonas chlororaphis 449.
25665785	1	4	theme	NMR	331:333	arg1	spectroscopy					335:346	(13)C NMR spectroscopy	325:346	(13)C NMR spectroscopy	325:346	O-Specific polysaccharides were obtained from the lipopolysaccharides isolated from the planktonic and biofilm cultures of Pseudomonas chlororaphis 449 and studied by composition analysis and 1D and 2D (1)H and (13)C NMR spectroscopy.
25665785	2	5	from	%	548:548	arg1	form					568:571	the planktonic form	553:571	the planktonic form	553:571	The following structure was established: -->4)-α-D-GalpNAc6Ac-(1-->3)-β-D-QuipNAc-(1-->6)-α-D-GlcpNAc-(1-->β-D-GlcpNAc-(1-->3) where the degree of non-stoichiometric 6-O-acetylation of GalNAc is ∼ 60% in the planktonic form or ∼ 10% in biofilm.
25665785	2	5	from	%	548:548	arg1	biofilm					585:591	biofilm	585:591	biofilm	585:591	The following structure was established: -->4)-α-D-GalpNAc6Ac-(1-->3)-β-D-QuipNAc-(1-->6)-α-D-GlcpNAc-(1-->β-D-GlcpNAc-(1-->3) where the degree of non-stoichiometric 6-O-acetylation of GalNAc is ∼ 60% in the planktonic form or ∼ 10% in biofilm.
25665785	0	6	theme	planktonic	49:58	arg1	cultures					72:79	planktonic and biofilm cultures	49:79	planktonic and biofilm cultures of Pseudomonas chlororaphis 449	49:111	Structure of the O-specific polysaccharides from planktonic and biofilm cultures of Pseudomonas chlororaphis 449.
25665785	1	7	theme	planktonic	202:211	arg1	cultures					225:232	the planktonic and biofilm cultures	198:232	cultures	225:232	O-Specific polysaccharides were obtained from the lipopolysaccharides isolated from the planktonic and biofilm cultures of Pseudomonas chlororaphis 449 and studied by composition analysis and 1D and 2D (1)H and (13)C NMR spectroscopy.
25665785	1	8	attach	isolated	184:191	arg2	lipopolysaccharides					164:182	the lipopolysaccharides	160:182	the lipopolysaccharides isolated from the planktonic and biofilm cultures of Pseudomonas chlororaphis 449	160:264	O-Specific polysaccharides were obtained from the lipopolysaccharides isolated from the planktonic and biofilm cultures of Pseudomonas chlororaphis 449 and studied by composition analysis and 1D and 2D (1)H and (13)C NMR spectroscopy.
25665785	1	8	attach	isolated	184:191	arg1	cultures					225:232	the planktonic and biofilm cultures	198:232	cultures	225:232	O-Specific polysaccharides were obtained from the lipopolysaccharides isolated from the planktonic and biofilm cultures of Pseudomonas chlororaphis 449 and studied by composition analysis and 1D and 2D (1)H and (13)C NMR spectroscopy.
25665785	2	9	from	%	580:580	arg1	form					568:571	the planktonic form	553:571	the planktonic form	553:571	The following structure was established: -->4)-α-D-GalpNAc6Ac-(1-->3)-β-D-QuipNAc-(1-->6)-α-D-GlcpNAc-(1-->β-D-GlcpNAc-(1-->3) where the degree of non-stoichiometric 6-O-acetylation of GalNAc is ∼ 60% in the planktonic form or ∼ 10% in biofilm.
25665785	2	9	from	%	580:580	arg1	biofilm					585:591	biofilm	585:591	biofilm	585:591	The following structure was established: -->4)-α-D-GalpNAc6Ac-(1-->3)-β-D-QuipNAc-(1-->6)-α-D-GlcpNAc-(1-->β-D-GlcpNAc-(1-->3) where the degree of non-stoichiometric 6-O-acetylation of GalNAc is ∼ 60% in the planktonic form or ∼ 10% in biofilm.
25665785	2	10	theme	-β-D-QuipNAc-	418:430	arg1	1-->β-D-GlcpNAc-					452:467	1-->β-D-GlcpNAc-	452:467	1-->β-D-GlcpNAc-	452:467	The following structure was established: -->4)-α-D-GalpNAc6Ac-(1-->3)-β-D-QuipNAc-(1-->6)-α-D-GlcpNAc-(1-->β-D-GlcpNAc-(1-->3) where the degree of non-stoichiometric 6-O-acetylation of GalNAc is ∼ 60% in the planktonic form or ∼ 10% in biofilm.
25665785	2	10	theme	-β-D-QuipNAc-	418:430	arg1	-α-D-GlcpNAc-					438:450	-->4)-α-D-GalpNAc6Ac-(1-->3)-β-D-QuipNAc-(1-->6)-α-D-GlcpNAc-	390:450	-->4)-α-D-GalpNAc6Ac-(1-->3)-β-D-QuipNAc-(1-->6)-α-D-GlcpNAc-(1-->β-D-GlcpNAc-(1-->3) where the degree of non-stoichiometric 6-O-acetylation of GalNAc is ∼ 60% in the planktonic form or ∼ 10% in biofilm	390:591	The following structure was established: -->4)-α-D-GalpNAc6Ac-(1-->3)-β-D-QuipNAc-(1-->6)-α-D-GlcpNAc-(1-->β-D-GlcpNAc-(1-->3) where the degree of non-stoichiometric 6-O-acetylation of GalNAc is ∼ 60% in the planktonic form or ∼ 10% in biofilm.
25665785	1	11	theme	biofilm	217:223	arg1	cultures					225:232	the planktonic and biofilm cultures	198:232	cultures	225:232	O-Specific polysaccharides were obtained from the lipopolysaccharides isolated from the planktonic and biofilm cultures of Pseudomonas chlororaphis 449 and studied by composition analysis and 1D and 2D (1)H and (13)C NMR spectroscopy.
25665785	0	12	theme	O-specific	17:26	arg1	polysaccharides					28:42	the O-specific polysaccharides	13:42	the O-specific polysaccharides from planktonic and biofilm cultures of Pseudomonas chlororaphis 449	13:111	Structure of the O-specific polysaccharides from planktonic and biofilm cultures of Pseudomonas chlororaphis 449.
25665785	2	13	theme	GalNAc	534:539	arg1	6-O-acetylation					515:529	non-stoichiometric 6-O-acetylation	496:529	non-stoichiometric 6-O-acetylation of GalNAc	496:539	The following structure was established: -->4)-α-D-GalpNAc6Ac-(1-->3)-β-D-QuipNAc-(1-->6)-α-D-GlcpNAc-(1-->β-D-GlcpNAc-(1-->3) where the degree of non-stoichiometric 6-O-acetylation of GalNAc is ∼ 60% in the planktonic form or ∼ 10% in biofilm.
25665785	2	14	theme	1-->3	412:416	arg1	1-->β-D-GlcpNAc-					452:467	1-->β-D-GlcpNAc-	452:467	1-->β-D-GlcpNAc-	452:467	The following structure was established: -->4)-α-D-GalpNAc6Ac-(1-->3)-β-D-QuipNAc-(1-->6)-α-D-GlcpNAc-(1-->β-D-GlcpNAc-(1-->3) where the degree of non-stoichiometric 6-O-acetylation of GalNAc is ∼ 60% in the planktonic form or ∼ 10% in biofilm.
25665785	2	14	theme	1-->3	412:416	arg1	-α-D-GlcpNAc-					438:450	-->4)-α-D-GalpNAc6Ac-(1-->3)-β-D-QuipNAc-(1-->6)-α-D-GlcpNAc-	390:450	-->4)-α-D-GalpNAc6Ac-(1-->3)-β-D-QuipNAc-(1-->6)-α-D-GlcpNAc-(1-->β-D-GlcpNAc-(1-->3) where the degree of non-stoichiometric 6-O-acetylation of GalNAc is ∼ 60% in the planktonic form or ∼ 10% in biofilm	390:591	The following structure was established: -->4)-α-D-GalpNAc6Ac-(1-->3)-β-D-QuipNAc-(1-->6)-α-D-GlcpNAc-(1-->β-D-GlcpNAc-(1-->3) where the degree of non-stoichiometric 6-O-acetylation of GalNAc is ∼ 60% in the planktonic form or ∼ 10% in biofilm.
25665785	1	15	theme	O-Specific	114:123	arg1	polysaccharides					125:139	O-Specific polysaccharides	114:139	O-Specific polysaccharides	114:139	O-Specific polysaccharides were obtained from the lipopolysaccharides isolated from the planktonic and biofilm cultures of Pseudomonas chlororaphis 449 and studied by composition analysis and 1D and 2D (1)H and (13)C NMR spectroscopy.
25665785	2	16	theme	-α-D-GalpNAc6Ac-	395:410	arg1	1-->β-D-GlcpNAc-					452:467	1-->β-D-GlcpNAc-	452:467	1-->β-D-GlcpNAc-	452:467	The following structure was established: -->4)-α-D-GalpNAc6Ac-(1-->3)-β-D-QuipNAc-(1-->6)-α-D-GlcpNAc-(1-->β-D-GlcpNAc-(1-->3) where the degree of non-stoichiometric 6-O-acetylation of GalNAc is ∼ 60% in the planktonic form or ∼ 10% in biofilm.
25665785	2	16	theme	-α-D-GalpNAc6Ac-	395:410	arg1	-α-D-GlcpNAc-					438:450	-->4)-α-D-GalpNAc6Ac-(1-->3)-β-D-QuipNAc-(1-->6)-α-D-GlcpNAc-	390:450	-->4)-α-D-GalpNAc6Ac-(1-->3)-β-D-QuipNAc-(1-->6)-α-D-GlcpNAc-(1-->β-D-GlcpNAc-(1-->3) where the degree of non-stoichiometric 6-O-acetylation of GalNAc is ∼ 60% in the planktonic form or ∼ 10% in biofilm	390:591	The following structure was established: -->4)-α-D-GalpNAc6Ac-(1-->3)-β-D-QuipNAc-(1-->6)-α-D-GlcpNAc-(1-->β-D-GlcpNAc-(1-->3) where the degree of non-stoichiometric 6-O-acetylation of GalNAc is ∼ 60% in the planktonic form or ∼ 10% in biofilm.
25665785	2	17	theme	6-O-acetylation	515:529	arg1	degree					486:491	the degree	482:491	the degree of non-stoichiometric 6-O-acetylation of GalNAc	482:539	The following structure was established: -->4)-α-D-GalpNAc6Ac-(1-->3)-β-D-QuipNAc-(1-->6)-α-D-GlcpNAc-(1-->β-D-GlcpNAc-(1-->3) where the degree of non-stoichiometric 6-O-acetylation of GalNAc is ∼ 60% in the planktonic form or ∼ 10% in biofilm.
25665785	2	17	theme	6-O-acetylation	515:529	arg1	%					548:548	∼ 60%	544:548	∼ 60% in the planktonic form	544:571	The following structure was established: -->4)-α-D-GalpNAc6Ac-(1-->3)-β-D-QuipNAc-(1-->6)-α-D-GlcpNAc-(1-->β-D-GlcpNAc-(1-->3) where the degree of non-stoichiometric 6-O-acetylation of GalNAc is ∼ 60% in the planktonic form or ∼ 10% in biofilm.
25665785	0	18	theme	polysaccharides	28:42	arg1	Structure					0:8	Structure	0:8	Structure of the O-specific polysaccharides from planktonic and biofilm cultures of Pseudomonas chlororaphis 449.	0:112	Structure of the O-specific polysaccharides from planktonic and biofilm cultures of Pseudomonas chlororaphis 449.
25665785	2	19	theme	non-stoichiometric	496:513	arg1	6-O-acetylation					515:529	non-stoichiometric 6-O-acetylation	496:529	non-stoichiometric 6-O-acetylation of GalNAc	496:539	The following structure was established: -->4)-α-D-GalpNAc6Ac-(1-->3)-β-D-QuipNAc-(1-->6)-α-D-GlcpNAc-(1-->β-D-GlcpNAc-(1-->3) where the degree of non-stoichiometric 6-O-acetylation of GalNAc is ∼ 60% in the planktonic form or ∼ 10% in biofilm.
25665785	0	20	from	cultures	72:79	arg1	Structure					0:8	Structure	0:8	Structure of the O-specific polysaccharides from planktonic and biofilm cultures of Pseudomonas chlororaphis 449.	0:112	Structure of the O-specific polysaccharides from planktonic and biofilm cultures of Pseudomonas chlororaphis 449.
25665785	0	20	from	cultures	72:79	arg1	polysaccharides					28:42	the O-specific polysaccharides	13:42	the O-specific polysaccharides from planktonic and biofilm cultures of Pseudomonas chlororaphis 449	13:111	Structure of the O-specific polysaccharides from planktonic and biofilm cultures of Pseudomonas chlororaphis 449.
25665785	0	21	from	Structure	0:8	arg1	cultures					72:79	planktonic and biofilm cultures	49:79	planktonic and biofilm cultures of Pseudomonas chlororaphis 449	49:111	Structure of the O-specific polysaccharides from planktonic and biofilm cultures of Pseudomonas chlororaphis 449.
25665785	1	22	dep	2D	313:314	arg1	H					319:319	H	319:319	H	319:319	O-Specific polysaccharides were obtained from the lipopolysaccharides isolated from the planktonic and biofilm cultures of Pseudomonas chlororaphis 449 and studied by composition analysis and 1D and 2D (1)H and (13)C NMR spectroscopy.
25665785	1	22	dep	2D	313:314	arg1	1					317:317	1	317:317	1	317:317	O-Specific polysaccharides were obtained from the lipopolysaccharides isolated from the planktonic and biofilm cultures of Pseudomonas chlororaphis 449 and studied by composition analysis and 1D and 2D (1)H and (13)C NMR spectroscopy.
25665785	1	22	dep	2D	313:314	arg1	spectroscopy					335:346	(13)C NMR spectroscopy	325:346	(13)C NMR spectroscopy	325:346	O-Specific polysaccharides were obtained from the lipopolysaccharides isolated from the planktonic and biofilm cultures of Pseudomonas chlororaphis 449 and studied by composition analysis and 1D and 2D (1)H and (13)C NMR spectroscopy.
25665785	2	23	dep	1-->β-D-GlcpNAc-	452:467	arg1	1-->3					469:473	1-->3	469:473	1-->3	469:473	The following structure was established: -->4)-α-D-GalpNAc6Ac-(1-->3)-β-D-QuipNAc-(1-->6)-α-D-GlcpNAc-(1-->β-D-GlcpNAc-(1-->3) where the degree of non-stoichiometric 6-O-acetylation of GalNAc is ∼ 60% in the planktonic form or ∼ 10% in biofilm.
25665785	2	24	theme	1-->6	432:436	arg1	1-->β-D-GlcpNAc-					452:467	1-->β-D-GlcpNAc-	452:467	1-->β-D-GlcpNAc-	452:467	The following structure was established: -->4)-α-D-GalpNAc6Ac-(1-->3)-β-D-QuipNAc-(1-->6)-α-D-GlcpNAc-(1-->β-D-GlcpNAc-(1-->3) where the degree of non-stoichiometric 6-O-acetylation of GalNAc is ∼ 60% in the planktonic form or ∼ 10% in biofilm.
25665785	2	24	theme	1-->6	432:436	arg1	-α-D-GlcpNAc-					438:450	-->4)-α-D-GalpNAc6Ac-(1-->3)-β-D-QuipNAc-(1-->6)-α-D-GlcpNAc-	390:450	-->4)-α-D-GalpNAc6Ac-(1-->3)-β-D-QuipNAc-(1-->6)-α-D-GlcpNAc-(1-->β-D-GlcpNAc-(1-->3) where the degree of non-stoichiometric 6-O-acetylation of GalNAc is ∼ 60% in the planktonic form or ∼ 10% in biofilm	390:591	The following structure was established: -->4)-α-D-GalpNAc6Ac-(1-->3)-β-D-QuipNAc-(1-->6)-α-D-GlcpNAc-(1-->β-D-GlcpNAc-(1-->3) where the degree of non-stoichiometric 6-O-acetylation of GalNAc is ∼ 60% in the planktonic form or ∼ 10% in biofilm.
25665785	1	25	theme	449	262:264	arg1	cultures					225:232	the planktonic and biofilm cultures	198:232	cultures	225:232	O-Specific polysaccharides were obtained from the lipopolysaccharides isolated from the planktonic and biofilm cultures of Pseudomonas chlororaphis 449 and studied by composition analysis and 1D and 2D (1)H and (13)C NMR spectroscopy.
25665785	2	26	theme	following	353:361	arg1	structure					363:371	The following structure	349:371	The following structure	349:371	The following structure was established: -->4)-α-D-GalpNAc6Ac-(1-->3)-β-D-QuipNAc-(1-->6)-α-D-GlcpNAc-(1-->β-D-GlcpNAc-(1-->3) where the degree of non-stoichiometric 6-O-acetylation of GalNAc is ∼ 60% in the planktonic form or ∼ 10% in biofilm.
25665785	0	27	theme	biofilm	64:70	arg1	cultures					72:79	planktonic and biofilm cultures	49:79	planktonic and biofilm cultures of Pseudomonas chlororaphis 449	49:111	Structure of the O-specific polysaccharides from planktonic and biofilm cultures of Pseudomonas chlororaphis 449.
25766808	0	0	theme	antioxidant	81:91	arg1	activity					93:100	antioxidant activity	81:100	antioxidant activity	81:100	Sorghum flour fractions: correlations among polysaccharides, phenolic compounds, antioxidant activity and glycemic index.
25766808	8	1	theme	RS	1022:1023	arg1	content					1025:1031	RS content	1022:1031	RS content	1022:1031	RS content was not correlated to EGI.
25766808	7	2	theme	antioxidant	866:876	arg1	activities					878:887	antioxidant activities	866:887	antioxidant activities	866:887	Phenolic compounds, specific flavonoids and antioxidant activities, as well as total, insoluble and soluble dietary fiber and β-glucans of sorghum flour samples were all negatively correlated to EGI.
25766808	3	3	theme	highest	447:453	arg1	β-glucan					476:483	the highest protein, lipid, ash, β-glucan, total and insoluble dietary fiber contents; and the lowest non-resistant and total starch contents	443:583	β-glucan	476:483	SB presented the highest protein, lipid, ash, β-glucan, total and insoluble dietary fiber contents; and the lowest non-resistant and total starch contents.
25766808	5	4	theme	antioxidant	699:709	arg1	activities					711:720	antioxidant activities	699:720	antioxidant activities	699:720	Phenolic compounds and antioxidant activities were concentrated in SB.
25766808	6	5	theme	EGI	751:753	arg1	values					755:760	The EGI values	747:760	The EGI values	747:760	The EGI values were: DSF 84.5 ± 0.41; WSF 77.2 ± 0.33; and SB 60.3 ± 0.78.
25766808	3	6	theme	protein	455:461	arg1	β-glucan					476:483	the highest protein, lipid, ash, β-glucan, total and insoluble dietary fiber contents; and the lowest non-resistant and total starch contents	443:583	β-glucan	476:483	SB presented the highest protein, lipid, ash, β-glucan, total and insoluble dietary fiber contents; and the lowest non-resistant and total starch contents.
25766808	3	7	theme	starch	569:574	arg1	contents					576:583	total starch contents	563:583	total starch contents	563:583	SB presented the highest protein, lipid, ash, β-glucan, total and insoluble dietary fiber contents; and the lowest non-resistant and total starch contents.
25766808	4	8	theme	starch	625:630	arg1	contents					632:639	The highest carbohydrate and resistant starch contents	586:639	contents	632:639	The highest carbohydrate and resistant starch contents were in DSF and WSF, respectively.
25766808	7	9	theme	flour	969:973	arg1	samples					975:981	sorghum flour samples	961:981	sorghum flour samples	961:981	Phenolic compounds, specific flavonoids and antioxidant activities, as well as total, insoluble and soluble dietary fiber and β-glucans of sorghum flour samples were all negatively correlated to EGI.
25766808	0	10	theme	glycemic	106:113	arg1	index					115:119	glycemic index	106:119	glycemic index	106:119	Sorghum flour fractions: correlations among polysaccharides, phenolic compounds, antioxidant activity and glycemic index.
25766808	7	11	theme	samples	975:981	arg1	activities					878:887	antioxidant activities	866:887	antioxidant activities	866:887	Phenolic compounds, specific flavonoids and antioxidant activities, as well as total, insoluble and soluble dietary fiber and β-glucans of sorghum flour samples were all negatively correlated to EGI.
25766808	7	11	theme	samples	975:981	arg1	fiber					938:942	insoluble and soluble dietary fiber	908:942	insoluble and soluble dietary fiber	908:942	Phenolic compounds, specific flavonoids and antioxidant activities, as well as total, insoluble and soluble dietary fiber and β-glucans of sorghum flour samples were all negatively correlated to EGI.
25766808	7	11	theme	samples	975:981	arg1	flavonoids					851:860	specific flavonoids	842:860	specific flavonoids	842:860	Phenolic compounds, specific flavonoids and antioxidant activities, as well as total, insoluble and soluble dietary fiber and β-glucans of sorghum flour samples were all negatively correlated to EGI.
25766808	7	11	theme	samples	975:981	arg1	compounds					831:839	Phenolic compounds	822:839	Phenolic compounds	822:839	Phenolic compounds, specific flavonoids and antioxidant activities, as well as total, insoluble and soluble dietary fiber and β-glucans of sorghum flour samples were all negatively correlated to EGI.
25766808	7	11	theme	samples	975:981	arg1	β-glucans					948:956	β-glucans	948:956	β-glucans of sorghum flour samples	948:981	Phenolic compounds, specific flavonoids and antioxidant activities, as well as total, insoluble and soluble dietary fiber and β-glucans of sorghum flour samples were all negatively correlated to EGI.
25766808	1	12	dep	Nutrients	122:130	arg1	compounds					154:162	phenolic compounds	145:162	phenolic compounds	145:162	Nutrients composition, phenolic compounds, antioxidant activity and estimated glycemic index (EGI) were evaluated in sorghum bran (SB) and decorticated sorghum flour (DSF), obtained by a rice-polisher, as well as whole sorghum flour (WSF).
25766808	1	12	dep	Nutrients	122:130	arg1	index					209:213	estimated glycemic index	190:213	estimated glycemic index (EGI)	190:219	Nutrients composition, phenolic compounds, antioxidant activity and estimated glycemic index (EGI) were evaluated in sorghum bran (SB) and decorticated sorghum flour (DSF), obtained by a rice-polisher, as well as whole sorghum flour (WSF).
25766808	1	12	dep	Nutrients	122:130	arg1	EGI					216:218	EGI	216:218	EGI	216:218	Nutrients composition, phenolic compounds, antioxidant activity and estimated glycemic index (EGI) were evaluated in sorghum bran (SB) and decorticated sorghum flour (DSF), obtained by a rice-polisher, as well as whole sorghum flour (WSF).
25766808	1	12	dep	Nutrients	122:130	arg1	Nutrients					122:130	Nutrients composition, phenolic compounds, antioxidant activity and estimated glycemic index (EGI)	122:219	Nutrients composition, phenolic compounds, antioxidant activity and estimated glycemic index (EGI)	122:219	Nutrients composition, phenolic compounds, antioxidant activity and estimated glycemic index (EGI) were evaluated in sorghum bran (SB) and decorticated sorghum flour (DSF), obtained by a rice-polisher, as well as whole sorghum flour (WSF).
25766808	1	12	dep	Nutrients	122:130	arg1	composition					132:142	composition	132:142	composition	132:142	Nutrients composition, phenolic compounds, antioxidant activity and estimated glycemic index (EGI) were evaluated in sorghum bran (SB) and decorticated sorghum flour (DSF), obtained by a rice-polisher, as well as whole sorghum flour (WSF).
25766808	1	12	dep	Nutrients	122:130	arg1	activity					177:184	antioxidant activity	165:184	antioxidant activity	165:184	Nutrients composition, phenolic compounds, antioxidant activity and estimated glycemic index (EGI) were evaluated in sorghum bran (SB) and decorticated sorghum flour (DSF), obtained by a rice-polisher, as well as whole sorghum flour (WSF).
25766808	0	13	theme	flour	8:12	arg1	fractions					14:22	Sorghum flour fractions	0:22	Sorghum flour fractions: correlations among polysaccharides, phenolic compounds, antioxidant activity and glycemic index.	0:120	Sorghum flour fractions: correlations among polysaccharides, phenolic compounds, antioxidant activity and glycemic index.
25766808	7	14	theme	Phenolic	822:829	arg1	compounds					831:839	Phenolic compounds	822:839	Phenolic compounds	822:839	Phenolic compounds, specific flavonoids and antioxidant activities, as well as total, insoluble and soluble dietary fiber and β-glucans of sorghum flour samples were all negatively correlated to EGI.
25766808	1	15	theme	estimated	190:198	arg1	EGI					216:218	EGI	216:218	EGI	216:218	Nutrients composition, phenolic compounds, antioxidant activity and estimated glycemic index (EGI) were evaluated in sorghum bran (SB) and decorticated sorghum flour (DSF), obtained by a rice-polisher, as well as whole sorghum flour (WSF).
25766808	1	15	theme	estimated	190:198	arg1	Nutrients					122:130	Nutrients composition, phenolic compounds, antioxidant activity and estimated glycemic index (EGI)	122:219	Nutrients composition, phenolic compounds, antioxidant activity and estimated glycemic index (EGI)	122:219	Nutrients composition, phenolic compounds, antioxidant activity and estimated glycemic index (EGI) were evaluated in sorghum bran (SB) and decorticated sorghum flour (DSF), obtained by a rice-polisher, as well as whole sorghum flour (WSF).
25766808	1	15	theme	estimated	190:198	arg1	index					209:213	estimated glycemic index	190:213	estimated glycemic index (EGI)	190:219	Nutrients composition, phenolic compounds, antioxidant activity and estimated glycemic index (EGI) were evaluated in sorghum bran (SB) and decorticated sorghum flour (DSF), obtained by a rice-polisher, as well as whole sorghum flour (WSF).
25766808	0	16	theme	Sorghum	0:6	arg1	fractions					14:22	Sorghum flour fractions	0:22	Sorghum flour fractions: correlations among polysaccharides, phenolic compounds, antioxidant activity and glycemic index.	0:120	Sorghum flour fractions: correlations among polysaccharides, phenolic compounds, antioxidant activity and glycemic index.
25766808	7	17	theme	sorghum	961:967	arg1	samples					975:981	sorghum flour samples	961:981	sorghum flour samples	961:981	Phenolic compounds, specific flavonoids and antioxidant activities, as well as total, insoluble and soluble dietary fiber and β-glucans of sorghum flour samples were all negatively correlated to EGI.
25766808	3	18	theme	insoluble	496:504	arg1	contents					520:527	insoluble dietary fiber contents	496:527	insoluble dietary fiber contents	496:527	SB presented the highest protein, lipid, ash, β-glucan, total and insoluble dietary fiber contents; and the lowest non-resistant and total starch contents.
25766808	1	19	theme	glycemic	200:207	arg1	EGI					216:218	EGI	216:218	EGI	216:218	Nutrients composition, phenolic compounds, antioxidant activity and estimated glycemic index (EGI) were evaluated in sorghum bran (SB) and decorticated sorghum flour (DSF), obtained by a rice-polisher, as well as whole sorghum flour (WSF).
25766808	1	19	theme	glycemic	200:207	arg1	Nutrients					122:130	Nutrients composition, phenolic compounds, antioxidant activity and estimated glycemic index (EGI)	122:219	Nutrients composition, phenolic compounds, antioxidant activity and estimated glycemic index (EGI)	122:219	Nutrients composition, phenolic compounds, antioxidant activity and estimated glycemic index (EGI) were evaluated in sorghum bran (SB) and decorticated sorghum flour (DSF), obtained by a rice-polisher, as well as whole sorghum flour (WSF).
25766808	1	19	theme	glycemic	200:207	arg1	index					209:213	estimated glycemic index	190:213	estimated glycemic index (EGI)	190:219	Nutrients composition, phenolic compounds, antioxidant activity and estimated glycemic index (EGI) were evaluated in sorghum bran (SB) and decorticated sorghum flour (DSF), obtained by a rice-polisher, as well as whole sorghum flour (WSF).
25766808	1	20	theme	decorticated	261:272	arg1	DSF					289:291	DSF	289:291	DSF	289:291	Nutrients composition, phenolic compounds, antioxidant activity and estimated glycemic index (EGI) were evaluated in sorghum bran (SB) and decorticated sorghum flour (DSF), obtained by a rice-polisher, as well as whole sorghum flour (WSF).
25766808	1	20	theme	decorticated	261:272	arg1	flour					282:286	decorticated sorghum flour	261:286	decorticated sorghum flour (DSF)	261:292	Nutrients composition, phenolic compounds, antioxidant activity and estimated glycemic index (EGI) were evaluated in sorghum bran (SB) and decorticated sorghum flour (DSF), obtained by a rice-polisher, as well as whole sorghum flour (WSF).
25766808	7	21	theme	specific	842:849	arg1	flavonoids					851:860	specific flavonoids	842:860	specific flavonoids	842:860	Phenolic compounds, specific flavonoids and antioxidant activities, as well as total, insoluble and soluble dietary fiber and β-glucans of sorghum flour samples were all negatively correlated to EGI.
25766808	1	22	theme	sorghum	274:280	arg1	DSF					289:291	DSF	289:291	DSF	289:291	Nutrients composition, phenolic compounds, antioxidant activity and estimated glycemic index (EGI) were evaluated in sorghum bran (SB) and decorticated sorghum flour (DSF), obtained by a rice-polisher, as well as whole sorghum flour (WSF).
25766808	1	22	theme	sorghum	274:280	arg1	flour					282:286	decorticated sorghum flour	261:286	decorticated sorghum flour (DSF)	261:292	Nutrients composition, phenolic compounds, antioxidant activity and estimated glycemic index (EGI) were evaluated in sorghum bran (SB) and decorticated sorghum flour (DSF), obtained by a rice-polisher, as well as whole sorghum flour (WSF).
25766808	1	23	theme	whole	335:339	arg1	flour					349:353	whole sorghum flour	335:353	whole sorghum flour (WSF)	335:359	Nutrients composition, phenolic compounds, antioxidant activity and estimated glycemic index (EGI) were evaluated in sorghum bran (SB) and decorticated sorghum flour (DSF), obtained by a rice-polisher, as well as whole sorghum flour (WSF).
25766808	1	23	theme	whole	335:339	arg1	WSF					356:358	WSF	356:358	WSF	356:358	Nutrients composition, phenolic compounds, antioxidant activity and estimated glycemic index (EGI) were evaluated in sorghum bran (SB) and decorticated sorghum flour (DSF), obtained by a rice-polisher, as well as whole sorghum flour (WSF).
25766808	1	24	theme	antioxidant	165:175	arg1	activity					177:184	antioxidant activity	165:184	antioxidant activity	165:184	Nutrients composition, phenolic compounds, antioxidant activity and estimated glycemic index (EGI) were evaluated in sorghum bran (SB) and decorticated sorghum flour (DSF), obtained by a rice-polisher, as well as whole sorghum flour (WSF).
25766808	1	24	theme	antioxidant	165:175	arg1	Nutrients					122:130	Nutrients composition, phenolic compounds, antioxidant activity and estimated glycemic index (EGI)	122:219	Nutrients composition, phenolic compounds, antioxidant activity and estimated glycemic index (EGI)	122:219	Nutrients composition, phenolic compounds, antioxidant activity and estimated glycemic index (EGI) were evaluated in sorghum bran (SB) and decorticated sorghum flour (DSF), obtained by a rice-polisher, as well as whole sorghum flour (WSF).
25766808	4	25	theme	resistant	615:623	arg1	contents					632:639	The highest carbohydrate and resistant starch contents	586:639	contents	632:639	The highest carbohydrate and resistant starch contents were in DSF and WSF, respectively.
25766808	7	26	theme	dietary	930:936	arg1	fiber					938:942	insoluble and soluble dietary fiber	908:942	insoluble and soluble dietary fiber	908:942	Phenolic compounds, specific flavonoids and antioxidant activities, as well as total, insoluble and soluble dietary fiber and β-glucans of sorghum flour samples were all negatively correlated to EGI.
25766808	1	27	theme	sorghum	341:347	arg1	flour					349:353	whole sorghum flour	335:353	whole sorghum flour (WSF)	335:359	Nutrients composition, phenolic compounds, antioxidant activity and estimated glycemic index (EGI) were evaluated in sorghum bran (SB) and decorticated sorghum flour (DSF), obtained by a rice-polisher, as well as whole sorghum flour (WSF).
25766808	1	27	theme	sorghum	341:347	arg1	WSF					356:358	WSF	356:358	WSF	356:358	Nutrients composition, phenolic compounds, antioxidant activity and estimated glycemic index (EGI) were evaluated in sorghum bran (SB) and decorticated sorghum flour (DSF), obtained by a rice-polisher, as well as whole sorghum flour (WSF).
25766808	4	28	theme	highest	590:596	arg1	carbohydrate					598:609	The highest carbohydrate and resistant starch contents	586:639	carbohydrate	598:609	The highest carbohydrate and resistant starch contents were in DSF and WSF, respectively.
25766808	3	29	theme	total	563:567	arg1	contents					576:583	total starch contents	563:583	total starch contents	563:583	SB presented the highest protein, lipid, ash, β-glucan, total and insoluble dietary fiber contents; and the lowest non-resistant and total starch contents.
25766808	3	30	theme	dietary	506:512	arg1	contents					520:527	insoluble dietary fiber contents	496:527	insoluble dietary fiber contents	496:527	SB presented the highest protein, lipid, ash, β-glucan, total and insoluble dietary fiber contents; and the lowest non-resistant and total starch contents.
25766808	2	31	theme	studied	394:400	arg1	parameters					402:411	the studied parameters	390:411	the studied parameters	390:411	Correlation between EGI and the studied parameters were determined.
25766808	7	32	theme	soluble	922:928	arg1	fiber					938:942	insoluble and soluble dietary fiber	908:942	insoluble and soluble dietary fiber	908:942	Phenolic compounds, specific flavonoids and antioxidant activities, as well as total, insoluble and soluble dietary fiber and β-glucans of sorghum flour samples were all negatively correlated to EGI.
25766808	3	33	theme	fiber	514:518	arg1	contents					520:527	insoluble dietary fiber contents	496:527	insoluble dietary fiber contents	496:527	SB presented the highest protein, lipid, ash, β-glucan, total and insoluble dietary fiber contents; and the lowest non-resistant and total starch contents.
25766808	3	34	theme	lowest	538:543	arg1	non-resistant					545:557	the lowest non-resistant	534:557	the lowest non-resistant	534:557	SB presented the highest protein, lipid, ash, β-glucan, total and insoluble dietary fiber contents; and the lowest non-resistant and total starch contents.
25766808	7	35	theme	insoluble	908:916	arg1	fiber					938:942	insoluble and soluble dietary fiber	908:942	insoluble and soluble dietary fiber	908:942	Phenolic compounds, specific flavonoids and antioxidant activities, as well as total, insoluble and soluble dietary fiber and β-glucans of sorghum flour samples were all negatively correlated to EGI.
25766808	0	36	dep	fractions	14:22	arg1	correlations					25:36	correlations	25:36	Sorghum flour fractions: correlations among polysaccharides, phenolic compounds, antioxidant activity and glycemic index.	0:120	Sorghum flour fractions: correlations among polysaccharides, phenolic compounds, antioxidant activity and glycemic index.
25766808	3	37	dep	β-glucan	476:483	arg1	contents					520:527	insoluble dietary fiber contents	496:527	insoluble dietary fiber contents	496:527	SB presented the highest protein, lipid, ash, β-glucan, total and insoluble dietary fiber contents; and the lowest non-resistant and total starch contents.
25766808	3	37	dep	β-glucan	476:483	arg1	lipid					464:468	the highest protein, lipid, ash, β-glucan, total and insoluble dietary fiber contents; and the lowest non-resistant and total starch contents	443:583	lipid	464:468	SB presented the highest protein, lipid, ash, β-glucan, total and insoluble dietary fiber contents; and the lowest non-resistant and total starch contents.
25766808	3	37	dep	β-glucan	476:483	arg1	total					486:490	total	486:490	total	486:490	SB presented the highest protein, lipid, ash, β-glucan, total and insoluble dietary fiber contents; and the lowest non-resistant and total starch contents.
25766808	3	37	dep	β-glucan	476:483	arg1	contents					576:583	total starch contents	563:583	total starch contents	563:583	SB presented the highest protein, lipid, ash, β-glucan, total and insoluble dietary fiber contents; and the lowest non-resistant and total starch contents.
25766808	3	37	dep	β-glucan	476:483	arg1	ash					471:473	the highest protein, lipid, ash, β-glucan, total and insoluble dietary fiber contents; and the lowest non-resistant and total starch contents	443:583	ash	471:473	SB presented the highest protein, lipid, ash, β-glucan, total and insoluble dietary fiber contents; and the lowest non-resistant and total starch contents.
25766808	3	37	dep	β-glucan	476:483	arg1	non-resistant					545:557	the lowest non-resistant	534:557	the lowest non-resistant	534:557	SB presented the highest protein, lipid, ash, β-glucan, total and insoluble dietary fiber contents; and the lowest non-resistant and total starch contents.
25766808	5	38	theme	Phenolic	676:683	arg1	compounds					685:693	Phenolic compounds	676:693	Phenolic compounds	676:693	Phenolic compounds and antioxidant activities were concentrated in SB.
25766808	1	39	theme	phenolic	145:152	arg1	compounds					154:162	phenolic compounds	145:162	phenolic compounds	145:162	Nutrients composition, phenolic compounds, antioxidant activity and estimated glycemic index (EGI) were evaluated in sorghum bran (SB) and decorticated sorghum flour (DSF), obtained by a rice-polisher, as well as whole sorghum flour (WSF).
25766808	0	40	theme	phenolic	61:68	arg1	compounds					70:78	phenolic compounds	61:78	phenolic compounds	61:78	Sorghum flour fractions: correlations among polysaccharides, phenolic compounds, antioxidant activity and glycemic index.
25766808	1	41	theme	sorghum	239:245	arg1	SB					253:254	SB	253:254	SB	253:254	Nutrients composition, phenolic compounds, antioxidant activity and estimated glycemic index (EGI) were evaluated in sorghum bran (SB) and decorticated sorghum flour (DSF), obtained by a rice-polisher, as well as whole sorghum flour (WSF).
25766808	1	41	theme	sorghum	239:245	arg1	bran					247:250	sorghum bran	239:250	sorghum bran (SB)	239:255	Nutrients composition, phenolic compounds, antioxidant activity and estimated glycemic index (EGI) were evaluated in sorghum bran (SB) and decorticated sorghum flour (DSF), obtained by a rice-polisher, as well as whole sorghum flour (WSF).
25301698	6	0	theme	higher	1216:1221	arg1	stability					1231:1239	higher thermal stability	1216:1239	higher thermal stability of cryogel	1216:1250	Thermal studies revealed higher thermal stability of cryogel.
25301698	4	1	theme	calculated	831:840	arg1	concentration					858:870	concentration	858:870	concentration of carboxymethyl tamarind kernel polysaccharide-6.0% (w/v), concentration of polyvinyl alcohol-8.53% (w/v) and freeze-thaw cycles-4, which provided cryogels with a release of 75.77% over a period of 6h	858:1072	The optimal calculated parameters were concentration of carboxymethyl tamarind kernel polysaccharide-6.0% (w/v), concentration of polyvinyl alcohol-8.53% (w/v) and freeze-thaw cycles-4, which provided cryogels with a release of 75.77% over a period of 6h.
25301698	4	1	theme	calculated	831:840	arg1	parameters					842:851	The optimal calculated parameters	819:851	The optimal calculated parameters	819:851	The optimal calculated parameters were concentration of carboxymethyl tamarind kernel polysaccharide-6.0% (w/v), concentration of polyvinyl alcohol-8.53% (w/v) and freeze-thaw cycles-4, which provided cryogels with a release of 75.77% over a period of 6h.
25301698	2	2	theme	alcohol	422:428	arg1	concentrations					348:361	concentrations	348:361	concentrations of carboxymethyl tamarind kernel polysaccharide, polyvinyl alcohol, and freeze-thaw cycles	348:452	The effect of concentrations of carboxymethyl tamarind kernel polysaccharide, polyvinyl alcohol, and freeze-thaw cycles on the % release of metronidazole was studied employing central composite experimental design.
25301698	4	3	theme	tamarind	889:896	arg1	kernel					898:903	carboxymethyl tamarind kernel	875:903	carboxymethyl tamarind kernel polysaccharide-6.0% (w/v)	875:929	The optimal calculated parameters were concentration of carboxymethyl tamarind kernel polysaccharide-6.0% (w/v), concentration of polyvinyl alcohol-8.53% (w/v) and freeze-thaw cycles-4, which provided cryogels with a release of 75.77% over a period of 6h.
25301698	4	3	theme	tamarind	889:896	arg1	concentration					932:944	concentration	932:944	concentration of polyvinyl alcohol-8.53% (w/v) and freeze-thaw cycles-4	932:1002	The optimal calculated parameters were concentration of carboxymethyl tamarind kernel polysaccharide-6.0% (w/v), concentration of polyvinyl alcohol-8.53% (w/v) and freeze-thaw cycles-4, which provided cryogels with a release of 75.77% over a period of 6h.
25301698	5	4	theme	diffraction	1170:1180	arg1	studies					1182:1188	X-ray diffraction studies	1164:1188	X-ray diffraction studies	1164:1188	The formation of cryogels was confirmed by Fourier-transformed infrared spectroscopy and X-ray diffraction studies.
25301698	5	5	theme	infrared	1138:1145	arg1	spectroscopy					1147:1158	Fourier-transformed infrared spectroscopy	1118:1158	Fourier-transformed infrared spectroscopy	1118:1158	The formation of cryogels was confirmed by Fourier-transformed infrared spectroscopy and X-ray diffraction studies.
25301698	3	6	theme	tamarind	622:629	arg1	polysaccharide					638:651	carboxymethyl tamarind kernel polysaccharide	608:651	carboxymethyl tamarind kernel polysaccharide	608:651	The result of the study revealed that the concentration of carboxymethyl tamarind kernel polysaccharide and interaction effect of concentrations of carboxymethyl tamarind kernel polysaccharide and polyvinyl alcohol influenced the release of metronidazole significantly.
25301698	4	7	theme	kernel	898:903	arg1	concentration					858:870	concentration	858:870	concentration of carboxymethyl tamarind kernel polysaccharide-6.0% (w/v), concentration of polyvinyl alcohol-8.53% (w/v) and freeze-thaw cycles-4, which provided cryogels with a release of 75.77% over a period of 6h	858:1072	The optimal calculated parameters were concentration of carboxymethyl tamarind kernel polysaccharide-6.0% (w/v), concentration of polyvinyl alcohol-8.53% (w/v) and freeze-thaw cycles-4, which provided cryogels with a release of 75.77% over a period of 6h.
25301698	4	7	theme	kernel	898:903	arg1	parameters					842:851	The optimal calculated parameters	819:851	The optimal calculated parameters	819:851	The optimal calculated parameters were concentration of carboxymethyl tamarind kernel polysaccharide-6.0% (w/v), concentration of polyvinyl alcohol-8.53% (w/v) and freeze-thaw cycles-4, which provided cryogels with a release of 75.77% over a period of 6h.
25301698	1	8	theme	release	316:322	arg1	behavior					324:331	release behavior	316:331	release behavior	316:331	The purpose of present study was to prepare composite hydrogels of carboxymethyl tamarind kernel polysaccharide and polyvinyl alcohol employing freeze thaw-treatment and evaluate them for release behavior.
25301698	3	9	theme	interaction	657:667	arg1	effect					669:674	interaction effect	657:674	interaction effect of concentrations of carboxymethyl tamarind kernel polysaccharide and polyvinyl alcohol	657:762	The result of the study revealed that the concentration of carboxymethyl tamarind kernel polysaccharide and interaction effect of concentrations of carboxymethyl tamarind kernel polysaccharide and polyvinyl alcohol influenced the release of metronidazole significantly.
25301698	4	10	theme	%	923:923	arg1	kernel					898:903	carboxymethyl tamarind kernel	875:903	carboxymethyl tamarind kernel polysaccharide-6.0% (w/v)	875:929	The optimal calculated parameters were concentration of carboxymethyl tamarind kernel polysaccharide-6.0% (w/v), concentration of polyvinyl alcohol-8.53% (w/v) and freeze-thaw cycles-4, which provided cryogels with a release of 75.77% over a period of 6h.
25301698	4	10	theme	%	923:923	arg1	concentration					932:944	concentration	932:944	concentration of polyvinyl alcohol-8.53% (w/v) and freeze-thaw cycles-4	932:1002	The optimal calculated parameters were concentration of carboxymethyl tamarind kernel polysaccharide-6.0% (w/v), concentration of polyvinyl alcohol-8.53% (w/v) and freeze-thaw cycles-4, which provided cryogels with a release of 75.77% over a period of 6h.
25301698	3	11	theme	kernel	631:636	arg1	polysaccharide					638:651	carboxymethyl tamarind kernel polysaccharide	608:651	carboxymethyl tamarind kernel polysaccharide	608:651	The result of the study revealed that the concentration of carboxymethyl tamarind kernel polysaccharide and interaction effect of concentrations of carboxymethyl tamarind kernel polysaccharide and polyvinyl alcohol influenced the release of metronidazole significantly.
25301698	3	12	theme	effect	669:674	arg1	concentration					591:603	the concentration	587:603	the concentration of carboxymethyl tamarind kernel polysaccharide and interaction effect of concentrations of carboxymethyl tamarind kernel polysaccharide and polyvinyl alcohol	587:762	The result of the study revealed that the concentration of carboxymethyl tamarind kernel polysaccharide and interaction effect of concentrations of carboxymethyl tamarind kernel polysaccharide and polyvinyl alcohol influenced the release of metronidazole significantly.
25301698	4	13	theme	optimal	823:829	arg1	concentration					858:870	concentration	858:870	concentration of carboxymethyl tamarind kernel polysaccharide-6.0% (w/v), concentration of polyvinyl alcohol-8.53% (w/v) and freeze-thaw cycles-4, which provided cryogels with a release of 75.77% over a period of 6h	858:1072	The optimal calculated parameters were concentration of carboxymethyl tamarind kernel polysaccharide-6.0% (w/v), concentration of polyvinyl alcohol-8.53% (w/v) and freeze-thaw cycles-4, which provided cryogels with a release of 75.77% over a period of 6h.
25301698	4	13	theme	optimal	823:829	arg1	parameters					842:851	The optimal calculated parameters	819:851	The optimal calculated parameters	819:851	The optimal calculated parameters were concentration of carboxymethyl tamarind kernel polysaccharide-6.0% (w/v), concentration of polyvinyl alcohol-8.53% (w/v) and freeze-thaw cycles-4, which provided cryogels with a release of 75.77% over a period of 6h.
25301698	1	14	theme	present	143:149	arg1	study					151:155	present study	143:155	present study	143:155	The purpose of present study was to prepare composite hydrogels of carboxymethyl tamarind kernel polysaccharide and polyvinyl alcohol employing freeze thaw-treatment and evaluate them for release behavior.
25301698	2	15	theme	metronidazole	474:486	arg1	release					463:469	the % release	457:469	the % release of metronidazole	457:486	The effect of concentrations of carboxymethyl tamarind kernel polysaccharide, polyvinyl alcohol, and freeze-thaw cycles on the % release of metronidazole was studied employing central composite experimental design.
25301698	1	16	theme	carboxymethyl	195:207	arg1	polysaccharide					225:238	carboxymethyl tamarind kernel polysaccharide	195:238	carboxymethyl tamarind kernel polysaccharide	195:238	The purpose of present study was to prepare composite hydrogels of carboxymethyl tamarind kernel polysaccharide and polyvinyl alcohol employing freeze thaw-treatment and evaluate them for release behavior.
25301698	2	17	theme	experimental	528:539	arg1	design					541:546	central composite experimental design	510:546	central composite experimental design	510:546	The effect of concentrations of carboxymethyl tamarind kernel polysaccharide, polyvinyl alcohol, and freeze-thaw cycles on the % release of metronidazole was studied employing central composite experimental design.
25301698	4	18	theme	polyvinyl	949:957	arg1	%					971:971	polyvinyl alcohol-8.53%	949:971	polyvinyl alcohol-8.53% (w/v)	949:977	The optimal calculated parameters were concentration of carboxymethyl tamarind kernel polysaccharide-6.0% (w/v), concentration of polyvinyl alcohol-8.53% (w/v) and freeze-thaw cycles-4, which provided cryogels with a release of 75.77% over a period of 6h.
25301698	4	18	theme	polyvinyl	949:957	arg1	w/v					974:976	w/v	974:976	w/v	974:976	The optimal calculated parameters were concentration of carboxymethyl tamarind kernel polysaccharide-6.0% (w/v), concentration of polyvinyl alcohol-8.53% (w/v) and freeze-thaw cycles-4, which provided cryogels with a release of 75.77% over a period of 6h.
25301698	3	19	theme	carboxymethyl	608:620	arg1	polysaccharide					638:651	carboxymethyl tamarind kernel polysaccharide	608:651	carboxymethyl tamarind kernel polysaccharide	608:651	The result of the study revealed that the concentration of carboxymethyl tamarind kernel polysaccharide and interaction effect of concentrations of carboxymethyl tamarind kernel polysaccharide and polyvinyl alcohol influenced the release of metronidazole significantly.
25301698	1	20	theme	tamarind	209:216	arg1	polysaccharide					225:238	carboxymethyl tamarind kernel polysaccharide	195:238	carboxymethyl tamarind kernel polysaccharide	195:238	The purpose of present study was to prepare composite hydrogels of carboxymethyl tamarind kernel polysaccharide and polyvinyl alcohol employing freeze thaw-treatment and evaluate them for release behavior.
25301698	2	21	theme	composite	518:526	arg1	design					541:546	central composite experimental design	510:546	central composite experimental design	510:546	The effect of concentrations of carboxymethyl tamarind kernel polysaccharide, polyvinyl alcohol, and freeze-thaw cycles on the % release of metronidazole was studied employing central composite experimental design.
25301698	4	22	theme	cycles-4	995:1002	arg1	kernel					898:903	carboxymethyl tamarind kernel	875:903	carboxymethyl tamarind kernel polysaccharide-6.0% (w/v)	875:929	The optimal calculated parameters were concentration of carboxymethyl tamarind kernel polysaccharide-6.0% (w/v), concentration of polyvinyl alcohol-8.53% (w/v) and freeze-thaw cycles-4, which provided cryogels with a release of 75.77% over a period of 6h.
25301698	4	22	theme	cycles-4	995:1002	arg1	concentration					932:944	concentration	932:944	concentration of polyvinyl alcohol-8.53% (w/v) and freeze-thaw cycles-4	932:1002	The optimal calculated parameters were concentration of carboxymethyl tamarind kernel polysaccharide-6.0% (w/v), concentration of polyvinyl alcohol-8.53% (w/v) and freeze-thaw cycles-4, which provided cryogels with a release of 75.77% over a period of 6h.
25301698	2	23	theme	tamarind	380:387	arg1	polysaccharide					396:409	carboxymethyl tamarind kernel polysaccharide	366:409	carboxymethyl tamarind kernel polysaccharide	366:409	The effect of concentrations of carboxymethyl tamarind kernel polysaccharide, polyvinyl alcohol, and freeze-thaw cycles on the % release of metronidazole was studied employing central composite experimental design.
25301698	1	24	theme	kernel	218:223	arg1	polysaccharide					225:238	carboxymethyl tamarind kernel polysaccharide	195:238	carboxymethyl tamarind kernel polysaccharide	195:238	The purpose of present study was to prepare composite hydrogels of carboxymethyl tamarind kernel polysaccharide and polyvinyl alcohol employing freeze thaw-treatment and evaluate them for release behavior.
25301698	2	25	theme	central	510:516	arg1	design					541:546	central composite experimental design	510:546	central composite experimental design	510:546	The effect of concentrations of carboxymethyl tamarind kernel polysaccharide, polyvinyl alcohol, and freeze-thaw cycles on the % release of metronidazole was studied employing central composite experimental design.
25301698	4	26	theme	freeze-thaw	983:993	arg1	cycles-4					995:1002	freeze-thaw cycles-4	983:1002	freeze-thaw cycles-4	983:1002	The optimal calculated parameters were concentration of carboxymethyl tamarind kernel polysaccharide-6.0% (w/v), concentration of polyvinyl alcohol-8.53% (w/v) and freeze-thaw cycles-4, which provided cryogels with a release of 75.77% over a period of 6h.
25301698	2	27	theme	carboxymethyl	366:378	arg1	polysaccharide					396:409	carboxymethyl tamarind kernel polysaccharide	366:409	carboxymethyl tamarind kernel polysaccharide	366:409	The effect of concentrations of carboxymethyl tamarind kernel polysaccharide, polyvinyl alcohol, and freeze-thaw cycles on the % release of metronidazole was studied employing central composite experimental design.
25301698	6	28	theme	thermal	1223:1229	arg1	stability					1231:1239	higher thermal stability	1216:1239	higher thermal stability of cryogel	1216:1250	Thermal studies revealed higher thermal stability of cryogel.
25301698	1	29	theme	polysaccharide	225:238	arg1	hydrogels					182:190	composite hydrogels	172:190	composite hydrogels of carboxymethyl tamarind kernel polysaccharide and polyvinyl alcohol employing freeze thaw-treatment	172:292	The purpose of present study was to prepare composite hydrogels of carboxymethyl tamarind kernel polysaccharide and polyvinyl alcohol employing freeze thaw-treatment and evaluate them for release behavior.
25301698	0	30	theme	tamarind	35:42	arg1	cryogels					84:91	carboxymethyl tamarind kernel polysaccharide-polyvinyl alcohol cryogels	21:91	carboxymethyl tamarind kernel polysaccharide-polyvinyl alcohol cryogels: preparation and characterization	21:125	Metronidazole loaded carboxymethyl tamarind kernel polysaccharide-polyvinyl alcohol cryogels: preparation and characterization.
25301698	4	31	theme	carboxymethyl	875:887	arg1	kernel					898:903	carboxymethyl tamarind kernel	875:903	carboxymethyl tamarind kernel polysaccharide-6.0% (w/v)	875:929	The optimal calculated parameters were concentration of carboxymethyl tamarind kernel polysaccharide-6.0% (w/v), concentration of polyvinyl alcohol-8.53% (w/v) and freeze-thaw cycles-4, which provided cryogels with a release of 75.77% over a period of 6h.
25301698	4	31	theme	carboxymethyl	875:887	arg1	concentration					932:944	concentration	932:944	concentration of polyvinyl alcohol-8.53% (w/v) and freeze-thaw cycles-4	932:1002	The optimal calculated parameters were concentration of carboxymethyl tamarind kernel polysaccharide-6.0% (w/v), concentration of polyvinyl alcohol-8.53% (w/v) and freeze-thaw cycles-4, which provided cryogels with a release of 75.77% over a period of 6h.
25301698	4	32	theme	%	1052:1052	arg1	release					1036:1042	a release	1034:1042	a release of 75.77%	1034:1052	The optimal calculated parameters were concentration of carboxymethyl tamarind kernel polysaccharide-6.0% (w/v), concentration of polyvinyl alcohol-8.53% (w/v) and freeze-thaw cycles-4, which provided cryogels with a release of 75.77% over a period of 6h.
25301698	5	33	theme	X-ray	1164:1168	arg1	studies					1182:1188	X-ray diffraction studies	1164:1188	X-ray diffraction studies	1164:1188	The formation of cryogels was confirmed by Fourier-transformed infrared spectroscopy and X-ray diffraction studies.
25301698	3	34	theme	polysaccharide	638:651	arg1	concentration					591:603	the concentration	587:603	the concentration of carboxymethyl tamarind kernel polysaccharide and interaction effect of concentrations of carboxymethyl tamarind kernel polysaccharide and polyvinyl alcohol	587:762	The result of the study revealed that the concentration of carboxymethyl tamarind kernel polysaccharide and interaction effect of concentrations of carboxymethyl tamarind kernel polysaccharide and polyvinyl alcohol influenced the release of metronidazole significantly.
25301698	4	35	theme	%	971:971	arg1	kernel					898:903	carboxymethyl tamarind kernel	875:903	carboxymethyl tamarind kernel polysaccharide-6.0% (w/v)	875:929	The optimal calculated parameters were concentration of carboxymethyl tamarind kernel polysaccharide-6.0% (w/v), concentration of polyvinyl alcohol-8.53% (w/v) and freeze-thaw cycles-4, which provided cryogels with a release of 75.77% over a period of 6h.
25301698	4	35	theme	%	971:971	arg1	concentration					932:944	concentration	932:944	concentration of polyvinyl alcohol-8.53% (w/v) and freeze-thaw cycles-4	932:1002	The optimal calculated parameters were concentration of carboxymethyl tamarind kernel polysaccharide-6.0% (w/v), concentration of polyvinyl alcohol-8.53% (w/v) and freeze-thaw cycles-4, which provided cryogels with a release of 75.77% over a period of 6h.
25301698	6	36	theme	Thermal	1191:1197	arg1	studies					1199:1205	Thermal studies	1191:1205	Thermal studies	1191:1205	Thermal studies revealed higher thermal stability of cryogel.
25301698	0	37	theme	carboxymethyl	21:33	arg1	cryogels					84:91	carboxymethyl tamarind kernel polysaccharide-polyvinyl alcohol cryogels	21:91	carboxymethyl tamarind kernel polysaccharide-polyvinyl alcohol cryogels: preparation and characterization	21:125	Metronidazole loaded carboxymethyl tamarind kernel polysaccharide-polyvinyl alcohol cryogels: preparation and characterization.
25301698	2	38	theme	concentrations	348:361	arg1	effect					338:343	The effect	334:343	The effect of concentrations of carboxymethyl tamarind kernel polysaccharide, polyvinyl alcohol, and freeze-thaw cycles on the % release of metronidazole	334:486	The effect of concentrations of carboxymethyl tamarind kernel polysaccharide, polyvinyl alcohol, and freeze-thaw cycles on the % release of metronidazole was studied employing central composite experimental design.
25301698	1	39	theme	polyvinyl	244:252	arg1	alcohol					254:260	polyvinyl alcohol	244:260	polyvinyl alcohol	244:260	The purpose of present study was to prepare composite hydrogels of carboxymethyl tamarind kernel polysaccharide and polyvinyl alcohol employing freeze thaw-treatment and evaluate them for release behavior.
25301698	0	40	theme	polysaccharide-polyvinyl	51:74	arg1	cryogels					84:91	carboxymethyl tamarind kernel polysaccharide-polyvinyl alcohol cryogels	21:91	carboxymethyl tamarind kernel polysaccharide-polyvinyl alcohol cryogels: preparation and characterization	21:125	Metronidazole loaded carboxymethyl tamarind kernel polysaccharide-polyvinyl alcohol cryogels: preparation and characterization.
25301698	3	41	theme	study	567:571	arg1	result					553:558	The result	549:558	The result of the study	549:571	The result of the study revealed that the concentration of carboxymethyl tamarind kernel polysaccharide and interaction effect of concentrations of carboxymethyl tamarind kernel polysaccharide and polyvinyl alcohol influenced the release of metronidazole significantly.
25301698	0	42	dep	cryogels	84:91	arg1	characterization					110:125	characterization	110:125	characterization	110:125	Metronidazole loaded carboxymethyl tamarind kernel polysaccharide-polyvinyl alcohol cryogels: preparation and characterization.
25301698	0	42	dep	cryogels	84:91	arg1	preparation					94:104	preparation	94:104	preparation	94:104	Metronidazole loaded carboxymethyl tamarind kernel polysaccharide-polyvinyl alcohol cryogels: preparation and characterization.
25301698	2	43	theme	cycles	447:452	arg1	concentrations					348:361	concentrations	348:361	concentrations of carboxymethyl tamarind kernel polysaccharide, polyvinyl alcohol, and freeze-thaw cycles	348:452	The effect of concentrations of carboxymethyl tamarind kernel polysaccharide, polyvinyl alcohol, and freeze-thaw cycles on the % release of metronidazole was studied employing central composite experimental design.
25301698	1	44	theme	alcohol	254:260	arg1	hydrogels					182:190	composite hydrogels	172:190	composite hydrogels of carboxymethyl tamarind kernel polysaccharide and polyvinyl alcohol employing freeze thaw-treatment	172:292	The purpose of present study was to prepare composite hydrogels of carboxymethyl tamarind kernel polysaccharide and polyvinyl alcohol employing freeze thaw-treatment and evaluate them for release behavior.
25301698	2	45	theme	%	461:461	arg1	release					463:469	the % release	457:469	the % release of metronidazole	457:486	The effect of concentrations of carboxymethyl tamarind kernel polysaccharide, polyvinyl alcohol, and freeze-thaw cycles on the % release of metronidazole was studied employing central composite experimental design.
25301698	0	46	theme	kernel	44:49	arg1	cryogels					84:91	carboxymethyl tamarind kernel polysaccharide-polyvinyl alcohol cryogels	21:91	carboxymethyl tamarind kernel polysaccharide-polyvinyl alcohol cryogels: preparation and characterization	21:125	Metronidazole loaded carboxymethyl tamarind kernel polysaccharide-polyvinyl alcohol cryogels: preparation and characterization.
25301698	3	47	theme	polyvinyl	746:754	arg1	alcohol					756:762	polyvinyl alcohol	746:762	polyvinyl alcohol	746:762	The result of the study revealed that the concentration of carboxymethyl tamarind kernel polysaccharide and interaction effect of concentrations of carboxymethyl tamarind kernel polysaccharide and polyvinyl alcohol influenced the release of metronidazole significantly.
25301698	3	48	theme	carboxymethyl	697:709	arg1	polysaccharide					727:740	carboxymethyl tamarind kernel polysaccharide	697:740	carboxymethyl tamarind kernel polysaccharide	697:740	The result of the study revealed that the concentration of carboxymethyl tamarind kernel polysaccharide and interaction effect of concentrations of carboxymethyl tamarind kernel polysaccharide and polyvinyl alcohol influenced the release of metronidazole significantly.
25301698	4	49	theme	6h	1071:1072	arg1	period					1061:1066	a period	1059:1066	a period of 6h	1059:1072	The optimal calculated parameters were concentration of carboxymethyl tamarind kernel polysaccharide-6.0% (w/v), concentration of polyvinyl alcohol-8.53% (w/v) and freeze-thaw cycles-4, which provided cryogels with a release of 75.77% over a period of 6h.
25301698	3	50	theme	alcohol	756:762	arg1	concentrations					679:692	concentrations	679:692	concentrations of carboxymethyl tamarind kernel polysaccharide and polyvinyl alcohol	679:762	The result of the study revealed that the concentration of carboxymethyl tamarind kernel polysaccharide and interaction effect of concentrations of carboxymethyl tamarind kernel polysaccharide and polyvinyl alcohol influenced the release of metronidazole significantly.
25301698	2	51	theme	polyvinyl	412:420	arg1	alcohol					422:428	polyvinyl alcohol	412:428	polyvinyl alcohol	412:428	The effect of concentrations of carboxymethyl tamarind kernel polysaccharide, polyvinyl alcohol, and freeze-thaw cycles on the % release of metronidazole was studied employing central composite experimental design.
25301698	0	52	theme	alcohol	76:82	arg1	cryogels					84:91	carboxymethyl tamarind kernel polysaccharide-polyvinyl alcohol cryogels	21:91	carboxymethyl tamarind kernel polysaccharide-polyvinyl alcohol cryogels: preparation and characterization	21:125	Metronidazole loaded carboxymethyl tamarind kernel polysaccharide-polyvinyl alcohol cryogels: preparation and characterization.
25301698	3	53	theme	metronidazole	790:802	arg1	release					779:785	the release	775:785	the release of metronidazole	775:802	The result of the study revealed that the concentration of carboxymethyl tamarind kernel polysaccharide and interaction effect of concentrations of carboxymethyl tamarind kernel polysaccharide and polyvinyl alcohol influenced the release of metronidazole significantly.
25301698	3	54	theme	concentrations	679:692	arg1	polysaccharide					638:651	carboxymethyl tamarind kernel polysaccharide	608:651	carboxymethyl tamarind kernel polysaccharide	608:651	The result of the study revealed that the concentration of carboxymethyl tamarind kernel polysaccharide and interaction effect of concentrations of carboxymethyl tamarind kernel polysaccharide and polyvinyl alcohol influenced the release of metronidazole significantly.
25301698	3	54	theme	concentrations	679:692	arg1	effect					669:674	interaction effect	657:674	interaction effect of concentrations of carboxymethyl tamarind kernel polysaccharide and polyvinyl alcohol	657:762	The result of the study revealed that the concentration of carboxymethyl tamarind kernel polysaccharide and interaction effect of concentrations of carboxymethyl tamarind kernel polysaccharide and polyvinyl alcohol influenced the release of metronidazole significantly.
25301698	1	55	theme	study	151:155	arg1	purpose					132:138	The purpose	128:138	The purpose of present study	128:155	The purpose of present study was to prepare composite hydrogels of carboxymethyl tamarind kernel polysaccharide and polyvinyl alcohol employing freeze thaw-treatment and evaluate them for release behavior.
25301698	4	56	with	cryogels	1020:1027	arg1	release					1036:1042	a release	1034:1042	a release of 75.77%	1034:1052	The optimal calculated parameters were concentration of carboxymethyl tamarind kernel polysaccharide-6.0% (w/v), concentration of polyvinyl alcohol-8.53% (w/v) and freeze-thaw cycles-4, which provided cryogels with a release of 75.77% over a period of 6h.
25301698	5	57	theme	Fourier-transformed	1118:1136	arg1	spectroscopy					1147:1158	Fourier-transformed infrared spectroscopy	1118:1158	Fourier-transformed infrared spectroscopy	1118:1158	The formation of cryogels was confirmed by Fourier-transformed infrared spectroscopy and X-ray diffraction studies.
25301698	4	58	dep	%	923:923	arg1	w/v					926:928	w/v	926:928	w/v	926:928	The optimal calculated parameters were concentration of carboxymethyl tamarind kernel polysaccharide-6.0% (w/v), concentration of polyvinyl alcohol-8.53% (w/v) and freeze-thaw cycles-4, which provided cryogels with a release of 75.77% over a period of 6h.
25301698	3	59	theme	polysaccharide	727:740	arg1	concentrations					679:692	concentrations	679:692	concentrations of carboxymethyl tamarind kernel polysaccharide and polyvinyl alcohol	679:762	The result of the study revealed that the concentration of carboxymethyl tamarind kernel polysaccharide and interaction effect of concentrations of carboxymethyl tamarind kernel polysaccharide and polyvinyl alcohol influenced the release of metronidazole significantly.
25301698	2	60	theme	polysaccharide	396:409	arg1	concentrations					348:361	concentrations	348:361	concentrations of carboxymethyl tamarind kernel polysaccharide, polyvinyl alcohol, and freeze-thaw cycles	348:452	The effect of concentrations of carboxymethyl tamarind kernel polysaccharide, polyvinyl alcohol, and freeze-thaw cycles on the % release of metronidazole was studied employing central composite experimental design.
25301698	4	61	theme	polysaccharide-6.0	905:922	arg1	%					923:923	%	923:923	%	923:923	The optimal calculated parameters were concentration of carboxymethyl tamarind kernel polysaccharide-6.0% (w/v), concentration of polyvinyl alcohol-8.53% (w/v) and freeze-thaw cycles-4, which provided cryogels with a release of 75.77% over a period of 6h.
25301698	1	62	theme	freeze	272:277	arg1	thaw-treatment					279:292	freeze thaw-treatment	272:292	freeze thaw-treatment	272:292	The purpose of present study was to prepare composite hydrogels of carboxymethyl tamarind kernel polysaccharide and polyvinyl alcohol employing freeze thaw-treatment and evaluate them for release behavior.
25301698	5	63	theme	cryogels	1092:1099	arg1	formation					1079:1087	The formation	1075:1087	The formation of cryogels	1075:1099	The formation of cryogels was confirmed by Fourier-transformed infrared spectroscopy and X-ray diffraction studies.
25301698	2	64	theme	kernel	389:394	arg1	polysaccharide					396:409	carboxymethyl tamarind kernel polysaccharide	366:409	carboxymethyl tamarind kernel polysaccharide	366:409	The effect of concentrations of carboxymethyl tamarind kernel polysaccharide, polyvinyl alcohol, and freeze-thaw cycles on the % release of metronidazole was studied employing central composite experimental design.
25301698	6	65	theme	cryogel	1244:1250	arg1	stability					1231:1239	higher thermal stability	1216:1239	higher thermal stability of cryogel	1216:1250	Thermal studies revealed higher thermal stability of cryogel.
25301698	3	66	theme	tamarind	711:718	arg1	polysaccharide					727:740	carboxymethyl tamarind kernel polysaccharide	697:740	carboxymethyl tamarind kernel polysaccharide	697:740	The result of the study revealed that the concentration of carboxymethyl tamarind kernel polysaccharide and interaction effect of concentrations of carboxymethyl tamarind kernel polysaccharide and polyvinyl alcohol influenced the release of metronidazole significantly.
25301698	2	67	theme	freeze-thaw	435:445	arg1	cycles					447:452	freeze-thaw cycles	435:452	freeze-thaw cycles	435:452	The effect of concentrations of carboxymethyl tamarind kernel polysaccharide, polyvinyl alcohol, and freeze-thaw cycles on the % release of metronidazole was studied employing central composite experimental design.
25301698	2	68	from	effect	338:343	arg1	release					463:469	the % release	457:469	the % release of metronidazole	457:486	The effect of concentrations of carboxymethyl tamarind kernel polysaccharide, polyvinyl alcohol, and freeze-thaw cycles on the % release of metronidazole was studied employing central composite experimental design.
25301698	3	69	theme	kernel	720:725	arg1	polysaccharide					727:740	carboxymethyl tamarind kernel polysaccharide	697:740	carboxymethyl tamarind kernel polysaccharide	697:740	The result of the study revealed that the concentration of carboxymethyl tamarind kernel polysaccharide and interaction effect of concentrations of carboxymethyl tamarind kernel polysaccharide and polyvinyl alcohol influenced the release of metronidazole significantly.
25301698	1	70	theme	composite	172:180	arg1	hydrogels					182:190	composite hydrogels	172:190	composite hydrogels of carboxymethyl tamarind kernel polysaccharide and polyvinyl alcohol employing freeze thaw-treatment	172:292	The purpose of present study was to prepare composite hydrogels of carboxymethyl tamarind kernel polysaccharide and polyvinyl alcohol employing freeze thaw-treatment and evaluate them for release behavior.
27561466	2	0	theme	corner	520:525	arg1	regions					527:533	the cell corner regions	511:533	the cell corner regions	511:533	Transversal-section Raman images showed that the fiber secondary walls were preferentially degraded, whereas the compound middle lamellae, including the cell corner regions, were mainly intact after 16 weeks incubation.
27561466	4	1	theme	structural	730:739	arg1	properties					741:750	structural properties	730:750	structural properties	730:750	From wet chemistry analysis, the yields and structural properties for both hemicellulosic and lignin fractions were not significantly altered.
27561466	6	2	theme	lignin	1071:1076	arg1	biomacromolecule					1078:1093	lignin biomacromolecule	1071:1093	lignin biomacromolecule	1071:1093	Additionally, the preferential degradation of S units in lignin biomacromolecule was further confirmed by composition analysis of cell wall phenolics and the integration of 2D NMR correlations in the aromatic region.
27561466	2	3	theme	cell	515:518	arg1	regions					527:533	the cell corner regions	511:533	the cell corner regions	511:533	Transversal-section Raman images showed that the fiber secondary walls were preferentially degraded, whereas the compound middle lamellae, including the cell corner regions, were mainly intact after 16 weeks incubation.
27561466	1	4	theme	lignin	159:164	arg1	fractions					166:174	hemicellulosic and lignin fractions	140:174	hemicellulosic and lignin fractions	140:174	On the concept of biorefinery, hemicellulosic and lignin fractions were isolated from white-rot fungal Trametes velutina D10149 biodegraded poplar, and the structural modification was elucidated in detail according to the different incubation duration.
27561466	0	5	with	process	78:84	arg1	fungal					101:106	fungal	101:106	fungal	101:106	Structural modification of hemicelluloses and lignin based on the biorefinery process with white-rot fungal.
27561466	5	6	theme	remarkable	946:955	arg1	decrement					957:965	the remarkable decrement	942:965	the remarkable decrement of hemicellulose and lignin molecular weights	942:1011	The synergistic effect of ligninolytic system finally obviously appeared after 16 weeks incubation, evidenced by the remarkable decrement of hemicellulose and lignin molecular weights.
27561466	6	7	theme	aromatic	1214:1221	arg1	region					1223:1228	the aromatic region	1210:1228	the aromatic region	1210:1228	Additionally, the preferential degradation of S units in lignin biomacromolecule was further confirmed by composition analysis of cell wall phenolics and the integration of 2D NMR correlations in the aromatic region.
27561466	1	8	theme	structural	265:274	arg1	modification					276:287	the structural modification	261:287	the structural modification	261:287	On the concept of biorefinery, hemicellulosic and lignin fractions were isolated from white-rot fungal Trametes velutina D10149 biodegraded poplar, and the structural modification was elucidated in detail according to the different incubation duration.
27561466	5	9	theme	molecular	995:1003	arg1	weights					1005:1011	molecular weights	995:1011	molecular weights	995:1011	The synergistic effect of ligninolytic system finally obviously appeared after 16 weeks incubation, evidenced by the remarkable decrement of hemicellulose and lignin molecular weights.
27561466	5	10	theme	hemicellulose	970:982	arg1	decrement					957:965	the remarkable decrement	942:965	the remarkable decrement of hemicellulose and lignin molecular weights	942:1011	The synergistic effect of ligninolytic system finally obviously appeared after 16 weeks incubation, evidenced by the remarkable decrement of hemicellulose and lignin molecular weights.
27561466	5	11	theme	ligninolytic	855:866	arg1	system					868:873	ligninolytic system	855:873	ligninolytic system	855:873	The synergistic effect of ligninolytic system finally obviously appeared after 16 weeks incubation, evidenced by the remarkable decrement of hemicellulose and lignin molecular weights.
27561466	4	12	dep	yields	719:724	arg1	the					715:717	the	715:717	the	715:717	From wet chemistry analysis, the yields and structural properties for both hemicellulosic and lignin fractions were not significantly altered.
27561466	6	13	theme	wall	1149:1152	arg1	phenolics					1154:1162	cell wall phenolics	1144:1162	cell wall phenolics	1144:1162	Additionally, the preferential degradation of S units in lignin biomacromolecule was further confirmed by composition analysis of cell wall phenolics and the integration of 2D NMR correlations in the aromatic region.
27561466	4	14	theme	chemistry	695:703	arg1	analysis					705:712	wet chemistry analysis	691:712	wet chemistry analysis	691:712	From wet chemistry analysis, the yields and structural properties for both hemicellulosic and lignin fractions were not significantly altered.
27561466	6	15	theme	S	1060:1060	arg1	units					1062:1066	S units	1060:1066	S units	1060:1066	Additionally, the preferential degradation of S units in lignin biomacromolecule was further confirmed by composition analysis of cell wall phenolics and the integration of 2D NMR correlations in the aromatic region.
27561466	6	16	theme	correlations	1194:1205	arg1	analysis					1132:1139	composition analysis	1120:1139	composition analysis of cell wall phenolics	1120:1162	Additionally, the preferential degradation of S units in lignin biomacromolecule was further confirmed by composition analysis of cell wall phenolics and the integration of 2D NMR correlations in the aromatic region.
27561466	6	16	theme	correlations	1194:1205	arg1	integration					1172:1182	the integration	1168:1182	the integration of 2D NMR correlations in the aromatic region	1168:1228	Additionally, the preferential degradation of S units in lignin biomacromolecule was further confirmed by composition analysis of cell wall phenolics and the integration of 2D NMR correlations in the aromatic region.
27561466	2	17	theme	compound	475:482	arg1	intact					548:553	intact	548:553	intact	548:553	Transversal-section Raman images showed that the fiber secondary walls were preferentially degraded, whereas the compound middle lamellae, including the cell corner regions, were mainly intact after 16 weeks incubation.
27561466	2	17	theme	compound	475:482	arg1	lamellae					491:498	the compound middle lamellae	471:498	the compound middle lamellae	471:498	Transversal-section Raman images showed that the fiber secondary walls were preferentially degraded, whereas the compound middle lamellae, including the cell corner regions, were mainly intact after 16 weeks incubation.
27561466	2	18	theme	secondary	417:425	arg1	walls					427:431	the fiber secondary walls	407:431	the fiber secondary walls	407:431	Transversal-section Raman images showed that the fiber secondary walls were preferentially degraded, whereas the compound middle lamellae, including the cell corner regions, were mainly intact after 16 weeks incubation.
27561466	2	19	theme	middle	484:489	arg1	intact					548:553	intact	548:553	intact	548:553	Transversal-section Raman images showed that the fiber secondary walls were preferentially degraded, whereas the compound middle lamellae, including the cell corner regions, were mainly intact after 16 weeks incubation.
27561466	2	19	theme	middle	484:489	arg1	lamellae					491:498	the compound middle lamellae	471:498	the compound middle lamellae	471:498	Transversal-section Raman images showed that the fiber secondary walls were preferentially degraded, whereas the compound middle lamellae, including the cell corner regions, were mainly intact after 16 weeks incubation.
27561466	4	20	theme	lignin	780:785	arg1	fractions					787:795	lignin fractions	780:795	lignin fractions	780:795	From wet chemistry analysis, the yields and structural properties for both hemicellulosic and lignin fractions were not significantly altered.
27561466	2	21	theme	fiber	411:415	arg1	walls					427:431	the fiber secondary walls	407:431	the fiber secondary walls	407:431	Transversal-section Raman images showed that the fiber secondary walls were preferentially degraded, whereas the compound middle lamellae, including the cell corner regions, were mainly intact after 16 weeks incubation.
27561466	0	22	theme	Structural	0:9	arg1	modification					11:22	Structural modification	0:22	Structural modification of hemicelluloses and lignin	0:51	Structural modification of hemicelluloses and lignin based on the biorefinery process with white-rot fungal.
27561466	6	23	theme	cell	1144:1147	arg1	phenolics					1154:1162	cell wall phenolics	1144:1162	cell wall phenolics	1144:1162	Additionally, the preferential degradation of S units in lignin biomacromolecule was further confirmed by composition analysis of cell wall phenolics and the integration of 2D NMR correlations in the aromatic region.
27561466	0	24	theme	hemicelluloses	27:40	arg1	modification					11:22	Structural modification	0:22	Structural modification of hemicelluloses and lignin	0:51	Structural modification of hemicelluloses and lignin based on the biorefinery process with white-rot fungal.
27561466	5	25	theme	system	868:873	arg1	effect					845:850	The synergistic effect	829:850	The synergistic effect of ligninolytic system	829:873	The synergistic effect of ligninolytic system finally obviously appeared after 16 weeks incubation, evidenced by the remarkable decrement of hemicellulose and lignin molecular weights.
27561466	6	26	from	analysis	1132:1139	arg1	region					1223:1228	the aromatic region	1210:1228	the aromatic region	1210:1228	Additionally, the preferential degradation of S units in lignin biomacromolecule was further confirmed by composition analysis of cell wall phenolics and the integration of 2D NMR correlations in the aromatic region.
27561466	6	27	theme	phenolics	1154:1162	arg1	analysis					1132:1139	composition analysis	1120:1139	composition analysis of cell wall phenolics	1120:1162	Additionally, the preferential degradation of S units in lignin biomacromolecule was further confirmed by composition analysis of cell wall phenolics and the integration of 2D NMR correlations in the aromatic region.
27561466	6	27	theme	phenolics	1154:1162	arg1	integration					1172:1182	the integration	1168:1182	the integration of 2D NMR correlations in the aromatic region	1168:1228	Additionally, the preferential degradation of S units in lignin biomacromolecule was further confirmed by composition analysis of cell wall phenolics and the integration of 2D NMR correlations in the aromatic region.
27561466	1	28	theme	white-rot	195:203	arg1	poplar					249:254	white-rot fungal Trametes velutina D10149 biodegraded poplar	195:254	white-rot fungal Trametes velutina D10149 biodegraded poplar	195:254	On the concept of biorefinery, hemicellulosic and lignin fractions were isolated from white-rot fungal Trametes velutina D10149 biodegraded poplar, and the structural modification was elucidated in detail according to the different incubation duration.
27561466	6	29	theme	NMR	1190:1192	arg1	correlations					1194:1205	2D NMR correlations	1187:1205	2D NMR correlations	1187:1205	Additionally, the preferential degradation of S units in lignin biomacromolecule was further confirmed by composition analysis of cell wall phenolics and the integration of 2D NMR correlations in the aromatic region.
27561466	6	30	theme	preferential	1032:1043	arg1	degradation					1045:1055	the preferential degradation	1028:1055	the preferential degradation of S units in lignin biomacromolecule	1028:1093	Additionally, the preferential degradation of S units in lignin biomacromolecule was further confirmed by composition analysis of cell wall phenolics and the integration of 2D NMR correlations in the aromatic region.
27561466	5	31	dep	hemicellulose	970:982	arg1	weights					1005:1011	molecular weights	995:1011	molecular weights	995:1011	The synergistic effect of ligninolytic system finally obviously appeared after 16 weeks incubation, evidenced by the remarkable decrement of hemicellulose and lignin molecular weights.
27561466	2	32	theme	Raman	382:386	arg1	images					388:393	Transversal-section Raman images	362:393	Transversal-section Raman images	362:393	Transversal-section Raman images showed that the fiber secondary walls were preferentially degraded, whereas the compound middle lamellae, including the cell corner regions, were mainly intact after 16 weeks incubation.
27561466	1	33	theme	fungal	205:210	arg1	poplar					249:254	white-rot fungal Trametes velutina D10149 biodegraded poplar	195:254	white-rot fungal Trametes velutina D10149 biodegraded poplar	195:254	On the concept of biorefinery, hemicellulosic and lignin fractions were isolated from white-rot fungal Trametes velutina D10149 biodegraded poplar, and the structural modification was elucidated in detail according to the different incubation duration.
27561466	0	34	theme	lignin	46:51	arg1	modification					11:22	Structural modification	0:22	Structural modification of hemicelluloses and lignin	0:51	Structural modification of hemicelluloses and lignin based on the biorefinery process with white-rot fungal.
27561466	2	35	theme	weeks	564:568	arg1	incubation					570:579	16 weeks incubation	561:579	16 weeks incubation	561:579	Transversal-section Raman images showed that the fiber secondary walls were preferentially degraded, whereas the compound middle lamellae, including the cell corner regions, were mainly intact after 16 weeks incubation.
27561466	6	36	theme	composition	1120:1130	arg1	analysis					1132:1139	composition analysis	1120:1139	composition analysis of cell wall phenolics	1120:1162	Additionally, the preferential degradation of S units in lignin biomacromolecule was further confirmed by composition analysis of cell wall phenolics and the integration of 2D NMR correlations in the aromatic region.
27561466	1	37	theme	Trametes	212:219	arg1	poplar					249:254	white-rot fungal Trametes velutina D10149 biodegraded poplar	195:254	white-rot fungal Trametes velutina D10149 biodegraded poplar	195:254	On the concept of biorefinery, hemicellulosic and lignin fractions were isolated from white-rot fungal Trametes velutina D10149 biodegraded poplar, and the structural modification was elucidated in detail according to the different incubation duration.
27561466	5	38	theme	synergistic	833:843	arg1	effect					845:850	The synergistic effect	829:850	The synergistic effect of ligninolytic system	829:873	The synergistic effect of ligninolytic system finally obviously appeared after 16 weeks incubation, evidenced by the remarkable decrement of hemicellulose and lignin molecular weights.
27561466	6	39	from	degradation	1045:1055	arg1	biomacromolecule					1078:1093	lignin biomacromolecule	1071:1093	lignin biomacromolecule	1071:1093	Additionally, the preferential degradation of S units in lignin biomacromolecule was further confirmed by composition analysis of cell wall phenolics and the integration of 2D NMR correlations in the aromatic region.
27561466	3	40	theme	secondary	670:678	arg1	wall					680:683	the fiber secondary wall	660:683	the fiber secondary wall	660:683	More importantly, lignin and carbohydrates were simultaneously removed within the fiber secondary wall.
27561466	1	41	theme	velutina	221:228	arg1	poplar					249:254	white-rot fungal Trametes velutina D10149 biodegraded poplar	195:254	white-rot fungal Trametes velutina D10149 biodegraded poplar	195:254	On the concept of biorefinery, hemicellulosic and lignin fractions were isolated from white-rot fungal Trametes velutina D10149 biodegraded poplar, and the structural modification was elucidated in detail according to the different incubation duration.
27561466	1	42	attach	isolated	181:188	arg1	poplar					249:254	white-rot fungal Trametes velutina D10149 biodegraded poplar	195:254	white-rot fungal Trametes velutina D10149 biodegraded poplar	195:254	On the concept of biorefinery, hemicellulosic and lignin fractions were isolated from white-rot fungal Trametes velutina D10149 biodegraded poplar, and the structural modification was elucidated in detail according to the different incubation duration.
27561466	1	42	attach	isolated	181:188	arg2	fractions					166:174	hemicellulosic and lignin fractions	140:174	hemicellulosic and lignin fractions	140:174	On the concept of biorefinery, hemicellulosic and lignin fractions were isolated from white-rot fungal Trametes velutina D10149 biodegraded poplar, and the structural modification was elucidated in detail according to the different incubation duration.
27561466	2	43	theme	Transversal-section	362:380	arg1	images					388:393	Transversal-section Raman images	362:393	Transversal-section Raman images	362:393	Transversal-section Raman images showed that the fiber secondary walls were preferentially degraded, whereas the compound middle lamellae, including the cell corner regions, were mainly intact after 16 weeks incubation.
27561466	1	44	theme	different	331:339	arg1	duration					352:359	the different incubation duration	327:359	the different incubation duration	327:359	On the concept of biorefinery, hemicellulosic and lignin fractions were isolated from white-rot fungal Trametes velutina D10149 biodegraded poplar, and the structural modification was elucidated in detail according to the different incubation duration.
27561466	5	45	theme	lignin	988:993	arg1	decrement					957:965	the remarkable decrement	942:965	the remarkable decrement of hemicellulose and lignin molecular weights	942:1011	The synergistic effect of ligninolytic system finally obviously appeared after 16 weeks incubation, evidenced by the remarkable decrement of hemicellulose and lignin molecular weights.
27561466	1	46	theme	D10149	230:235	arg1	poplar					249:254	white-rot fungal Trametes velutina D10149 biodegraded poplar	195:254	white-rot fungal Trametes velutina D10149 biodegraded poplar	195:254	On the concept of biorefinery, hemicellulosic and lignin fractions were isolated from white-rot fungal Trametes velutina D10149 biodegraded poplar, and the structural modification was elucidated in detail according to the different incubation duration.
27561466	3	47	theme	fiber	664:668	arg1	wall					680:683	the fiber secondary wall	660:683	the fiber secondary wall	660:683	More importantly, lignin and carbohydrates were simultaneously removed within the fiber secondary wall.
27561466	6	48	from	integration	1172:1182	arg1	region					1223:1228	the aromatic region	1210:1228	the aromatic region	1210:1228	Additionally, the preferential degradation of S units in lignin biomacromolecule was further confirmed by composition analysis of cell wall phenolics and the integration of 2D NMR correlations in the aromatic region.
27561466	1	49	theme	biorefinery	127:137	arg1	concept					116:122	the concept	112:122	the concept of biorefinery	112:137	On the concept of biorefinery, hemicellulosic and lignin fractions were isolated from white-rot fungal Trametes velutina D10149 biodegraded poplar, and the structural modification was elucidated in detail according to the different incubation duration.
27561466	1	50	theme	biodegraded	237:247	arg1	poplar					249:254	white-rot fungal Trametes velutina D10149 biodegraded poplar	195:254	white-rot fungal Trametes velutina D10149 biodegraded poplar	195:254	On the concept of biorefinery, hemicellulosic and lignin fractions were isolated from white-rot fungal Trametes velutina D10149 biodegraded poplar, and the structural modification was elucidated in detail according to the different incubation duration.
27561466	0	51	theme	biorefinery	66:76	arg1	process					78:84	the biorefinery process	62:84	the biorefinery process with white-rot fungal	62:106	Structural modification of hemicelluloses and lignin based on the biorefinery process with white-rot fungal.
27561466	5	52	theme	weeks	911:915	arg1	incubation					917:926	16 weeks incubation	908:926	16 weeks incubation	908:926	The synergistic effect of ligninolytic system finally obviously appeared after 16 weeks incubation, evidenced by the remarkable decrement of hemicellulose and lignin molecular weights.
27561466	5	53	dep	appeared	893:900	arg1	evidenced					929:937	evidenced	929:937	evidenced by the remarkable decrement of hemicellulose and lignin molecular weights	929:1011	The synergistic effect of ligninolytic system finally obviously appeared after 16 weeks incubation, evidenced by the remarkable decrement of hemicellulose and lignin molecular weights.
27561466	6	54	theme	2D	1187:1188	arg1	correlations					1194:1205	2D NMR correlations	1187:1205	2D NMR correlations	1187:1205	Additionally, the preferential degradation of S units in lignin biomacromolecule was further confirmed by composition analysis of cell wall phenolics and the integration of 2D NMR correlations in the aromatic region.
27561466	4	55	theme	wet	691:693	arg1	analysis					705:712	wet chemistry analysis	691:712	wet chemistry analysis	691:712	From wet chemistry analysis, the yields and structural properties for both hemicellulosic and lignin fractions were not significantly altered.
27561466	6	56	theme	units	1062:1066	arg1	degradation					1045:1055	the preferential degradation	1028:1055	the preferential degradation of S units in lignin biomacromolecule	1028:1093	Additionally, the preferential degradation of S units in lignin biomacromolecule was further confirmed by composition analysis of cell wall phenolics and the integration of 2D NMR correlations in the aromatic region.
27561466	1	57	theme	incubation	341:350	arg1	duration					352:359	the different incubation duration	327:359	the different incubation duration	327:359	On the concept of biorefinery, hemicellulosic and lignin fractions were isolated from white-rot fungal Trametes velutina D10149 biodegraded poplar, and the structural modification was elucidated in detail according to the different incubation duration.
27561466	1	58	theme	hemicellulosic	140:153	arg1	fractions					166:174	hemicellulosic and lignin fractions	140:174	hemicellulosic and lignin fractions	140:174	On the concept of biorefinery, hemicellulosic and lignin fractions were isolated from white-rot fungal Trametes velutina D10149 biodegraded poplar, and the structural modification was elucidated in detail according to the different incubation duration.
28235467	0	0	theme	picoalgae	80:88	arg1	tauri					103:107	the picoalgae Ostreococcus tauri	76:107	the picoalgae Ostreococcus tauri	76:107	Identification and characterization of a novel starch branching enzyme from the picoalgae Ostreococcus tauri.
28235467	0	1	from	characterization	19:34	arg1	tauri					103:107	the picoalgae Ostreococcus tauri	76:107	the picoalgae Ostreococcus tauri	76:107	Identification and characterization of a novel starch branching enzyme from the picoalgae Ostreococcus tauri.
28235467	6	2	theme	acids	715:719	arg1	protein					721:727	The resulting 1058 amino acids protein	690:727	The resulting 1058 amino acids protein	690:727	The resulting 1058 amino acids protein has two in tandem carbohydrate binding domains (CBMs, from the CBM41 and CBM48 families) at the N-terminal (residues 64-403) followed by the C-terminal catalytic domain (residues 426-1058).
28235467	6	3	from	two	733:735	arg1	domains					768:774	tandem carbohydrate binding domains	740:774	tandem carbohydrate binding domains (CBMs, from the CBM41 and CBM48 families)	740:816	The resulting 1058 amino acids protein has two in tandem carbohydrate binding domains (CBMs, from the CBM41 and CBM48 families) at the N-terminal (residues 64-403) followed by the C-terminal catalytic domain (residues 426-1058).
28235467	9	4	theme	enzyme	1348:1353	arg1	function					1355:1362	enzyme function	1348:1362	enzyme function	1348:1362	Our results suggest that the Ot01g03030 gene codifies for a functional BE containing two CBMs from CBM41 and CBM48 families which are critical for enzyme function and regulation.
28235467	6	5	theme	catalytic	881:889	arg1	residues					899:906	residues 426-1058	899:915	residues 426-1058	899:915	The resulting 1058 amino acids protein has two in tandem carbohydrate binding domains (CBMs, from the CBM41 and CBM48 families) at the N-terminal (residues 64-403) followed by the C-terminal catalytic domain (residues 426-1058).
28235467	6	5	theme	catalytic	881:889	arg1	domain					891:896	the C-terminal catalytic domain	866:896	the C-terminal catalytic domain (residues 426-1058)	866:916	The resulting 1058 amino acids protein has two in tandem carbohydrate binding domains (CBMs, from the CBM41 and CBM48 families) at the N-terminal (residues 64-403) followed by the C-terminal catalytic domain (residues 426-1058).
28235467	6	6	theme	C-terminal	870:879	arg1	residues					899:906	residues 426-1058	899:915	residues 426-1058	899:915	The resulting 1058 amino acids protein has two in tandem carbohydrate binding domains (CBMs, from the CBM41 and CBM48 families) at the N-terminal (residues 64-403) followed by the C-terminal catalytic domain (residues 426-1058).
28235467	6	6	theme	C-terminal	870:879	arg1	domain					891:896	the C-terminal catalytic domain	866:896	the C-terminal catalytic domain (residues 426-1058)	866:916	The resulting 1058 amino acids protein has two in tandem carbohydrate binding domains (CBMs, from the CBM41 and CBM48 families) at the N-terminal (residues 64-403) followed by the C-terminal catalytic domain (residues 426-1058).
28235467	4	7	theme	Ot01g03030	463:472	arg1	gene					474:477	the Ot01g03030 gene	459:477	the Ot01g03030 gene	459:477	Herein, we describe the function of the Ot01g03030 gene from the picoalgae Ostreococcus tauri.
28235467	0	8	from	Identification	0:13	arg1	tauri					103:107	the picoalgae Ostreococcus tauri	76:107	the picoalgae Ostreococcus tauri	76:107	Identification and characterization of a novel starch branching enzyme from the picoalgae Ostreococcus tauri.
28235467	5	9	theme	branching	667:675	arg1	gene					652:655	this gene	647:655	this gene	647:655	Although in silico analysis suggested that this gene codes for a starch debranching enzyme, our biochemical studies support that this gene encodes a branching enzyme (BE).
28235467	5	9	theme	branching	667:675	arg1	enzyme					677:682	a branching enzyme	665:682	a branching enzyme (BE)	665:687	Although in silico analysis suggested that this gene codes for a starch debranching enzyme, our biochemical studies support that this gene encodes a branching enzyme (BE).
28235467	6	10	theme	CBM41	792:796	arg1	families					808:815	the CBM41 and CBM48 families	788:815	families	808:815	The resulting 1058 amino acids protein has two in tandem carbohydrate binding domains (CBMs, from the CBM41 and CBM48 families) at the N-terminal (residues 64-403) followed by the C-terminal catalytic domain (residues 426-1058).
28235467	7	11	theme	truncated	938:946	arg1	isoforms					948:955	the BE truncated isoforms	931:955	the BE truncated isoforms	931:955	Analysis of the BE truncated isoforms show that the CBMs bind differentially to whole starch, amylose or amylopectin.
28235467	9	12	theme	Ot01g03030	1230:1239	arg1	gene					1241:1244	the Ot01g03030 gene	1226:1244	the Ot01g03030 gene	1226:1244	Our results suggest that the Ot01g03030 gene codifies for a functional BE containing two CBMs from CBM41 and CBM48 families which are critical for enzyme function and regulation.
28235467	3	13	theme	final	382:386	arg1	composition					388:398	the final composition	378:398	the final composition of the starch granule	378:420	This modification determines the structure of amylopectin thus arranging the final composition of the starch granule.
28235467	4	14	from	tauri	511:515	arg1	function					447:454	the function	443:454	the function of the Ot01g03030 gene from the picoalgae Ostreococcus tauri	443:515	Herein, we describe the function of the Ot01g03030 gene from the picoalgae Ostreococcus tauri.
28235467	5	15	theme	biochemical	614:624	arg1	studies					626:632	our biochemical studies	610:632	our biochemical studies	610:632	Although in silico analysis suggested that this gene codes for a starch debranching enzyme, our biochemical studies support that this gene encodes a branching enzyme (BE).
28235467	6	16	theme	amino	709:713	arg1	acids					715:719	The resulting 1058 amino acids	690:719	The resulting 1058 amino acids protein	690:727	The resulting 1058 amino acids protein has two in tandem carbohydrate binding domains (CBMs, from the CBM41 and CBM48 families) at the N-terminal (residues 64-403) followed by the C-terminal catalytic domain (residues 426-1058).
28235467	8	17	theme	catalytic	1104:1112	arg1	activity					1114:1121	no catalytic activity	1101:1121	no catalytic activity	1101:1121	Furthermore, both CBMs seem to be essential for BE activity, as no catalytic activity was detected in the truncated enzyme comprising only by the catalytic domain.
28235467	8	18	dep	activity	1088:1095	arg1	BE					1085:1086	BE	1085:1086	BE	1085:1086	Furthermore, both CBMs seem to be essential for BE activity, as no catalytic activity was detected in the truncated enzyme comprising only by the catalytic domain.
28235467	6	19	dep	domains	768:774	arg1	CBMs					777:780	CBMs	777:780	CBMs	777:780	The resulting 1058 amino acids protein has two in tandem carbohydrate binding domains (CBMs, from the CBM41 and CBM48 families) at the N-terminal (residues 64-403) followed by the C-terminal catalytic domain (residues 426-1058).
28235467	4	20	theme	Ostreococcus	498:509	arg1	tauri					511:515	the picoalgae Ostreococcus tauri	484:515	the picoalgae Ostreococcus tauri	484:515	Herein, we describe the function of the Ot01g03030 gene from the picoalgae Ostreococcus tauri.
28235467	6	21	from	families	808:815	arg1	CBMs					777:780	CBMs	777:780	CBMs	777:780	The resulting 1058 amino acids protein has two in tandem carbohydrate binding domains (CBMs, from the CBM41 and CBM48 families) at the N-terminal (residues 64-403) followed by the C-terminal catalytic domain (residues 426-1058).
28235467	2	22	theme	branches	270:277	arg1	addition					245:252	the addition	241:252	the addition of α-1,6-glucan branches to the α-1,4-polyglucans	241:302	This enzyme participates in starch granule assembly by the addition of α-1,6-glucan branches to the α-1,4-polyglucans.
28235467	6	23	theme	resulting	694:702	arg1	acids					715:719	The resulting 1058 amino acids	690:719	The resulting 1058 amino acids protein	690:727	The resulting 1058 amino acids protein has two in tandem carbohydrate binding domains (CBMs, from the CBM41 and CBM48 families) at the N-terminal (residues 64-403) followed by the C-terminal catalytic domain (residues 426-1058).
28235467	4	24	theme	picoalgae	488:496	arg1	tauri					511:515	the picoalgae Ostreococcus tauri	484:515	the picoalgae Ostreococcus tauri	484:515	Herein, we describe the function of the Ot01g03030 gene from the picoalgae Ostreococcus tauri.
28235467	5	25	theme	starch	583:588	arg1	enzyme					602:607	a starch debranching enzyme	581:607	a starch debranching enzyme	581:607	Although in silico analysis suggested that this gene codes for a starch debranching enzyme, our biochemical studies support that this gene encodes a branching enzyme (BE).
28235467	5	26	theme	in	527:528	arg1	analysis					537:544	in silico analysis	527:544	in silico analysis	527:544	Although in silico analysis suggested that this gene codes for a starch debranching enzyme, our biochemical studies support that this gene encodes a branching enzyme (BE).
28235467	8	27	theme	catalytic	1183:1191	arg1	domain					1193:1198	the catalytic domain	1179:1198	the catalytic domain	1179:1198	Furthermore, both CBMs seem to be essential for BE activity, as no catalytic activity was detected in the truncated enzyme comprising only by the catalytic domain.
28235467	3	28	theme	starch	407:412	arg1	granule					414:420	the starch granule	403:420	the starch granule	403:420	This modification determines the structure of amylopectin thus arranging the final composition of the starch granule.
28235467	5	29	theme	debranching	590:600	arg1	enzyme					602:607	a starch debranching enzyme	581:607	a starch debranching enzyme	581:607	Although in silico analysis suggested that this gene codes for a starch debranching enzyme, our biochemical studies support that this gene encodes a branching enzyme (BE).
28235467	8	30	located	detected	1127:1134	arg2	activity					1114:1121	no catalytic activity	1101:1121	no catalytic activity	1101:1121	Furthermore, both CBMs seem to be essential for BE activity, as no catalytic activity was detected in the truncated enzyme comprising only by the catalytic domain.
28235467	8	30	located	detected	1127:1134	arg1	enzyme					1153:1158	the truncated enzyme	1139:1158	the truncated enzyme comprising only by the catalytic domain	1139:1198	Furthermore, both CBMs seem to be essential for BE activity, as no catalytic activity was detected in the truncated enzyme comprising only by the catalytic domain.
28235467	8	31	theme	truncated	1143:1151	arg1	enzyme					1153:1158	the truncated enzyme	1139:1158	the truncated enzyme comprising only by the catalytic domain	1139:1198	Furthermore, both CBMs seem to be essential for BE activity, as no catalytic activity was detected in the truncated enzyme comprising only by the catalytic domain.
28235467	6	32	dep	residues	899:906	arg1	426-1058					908:915	426-1058	908:915	426-1058	908:915	The resulting 1058 amino acids protein has two in tandem carbohydrate binding domains (CBMs, from the CBM41 and CBM48 families) at the N-terminal (residues 64-403) followed by the C-terminal catalytic domain (residues 426-1058).
28235467	1	33	theme	Starch	110:115	arg1	protein					156:162	a highly conserved protein	137:162	a highly conserved protein from plants to algae	137:183	Starch branching enzyme is a highly conserved protein from plants to algae.
28235467	1	33	theme	Starch	110:115	arg1	enzyme					127:132	Starch branching enzyme	110:132	Starch branching enzyme	110:132	Starch branching enzyme is a highly conserved protein from plants to algae.
28235467	0	34	theme	novel	41:45	arg1	enzyme					64:69	a novel starch branching enzyme	39:69	a novel starch branching enzyme from the picoalgae Ostreococcus tauri	39:107	Identification and characterization of a novel starch branching enzyme from the picoalgae Ostreococcus tauri.
28235467	0	35	theme	enzyme	64:69	arg1	characterization					19:34	characterization	19:34	characterization	19:34	Identification and characterization of a novel starch branching enzyme from the picoalgae Ostreococcus tauri.
28235467	0	35	theme	enzyme	64:69	arg1	Identification					0:13	Identification	0:13	Identification	0:13	Identification and characterization of a novel starch branching enzyme from the picoalgae Ostreococcus tauri.
28235467	4	36	theme	gene	474:477	arg1	function					447:454	the function	443:454	the function of the Ot01g03030 gene from the picoalgae Ostreococcus tauri	443:515	Herein, we describe the function of the Ot01g03030 gene from the picoalgae Ostreococcus tauri.
28235467	5	37	dep	enzyme	677:682	arg1	BE					685:686	BE	685:686	BE	685:686	Although in silico analysis suggested that this gene codes for a starch debranching enzyme, our biochemical studies support that this gene encodes a branching enzyme (BE).
28235467	6	38	theme	binding	760:766	arg1	domains					768:774	tandem carbohydrate binding domains	740:774	tandem carbohydrate binding domains (CBMs, from the CBM41 and CBM48 families)	740:816	The resulting 1058 amino acids protein has two in tandem carbohydrate binding domains (CBMs, from the CBM41 and CBM48 families) at the N-terminal (residues 64-403) followed by the C-terminal catalytic domain (residues 426-1058).
28235467	3	39	theme	granule	414:420	arg1	composition					388:398	the final composition	378:398	the final composition of the starch granule	378:420	This modification determines the structure of amylopectin thus arranging the final composition of the starch granule.
28235467	7	40	theme	whole	999:1003	arg1	starch					1005:1010	whole starch	999:1010	whole starch	999:1010	Analysis of the BE truncated isoforms show that the CBMs bind differentially to whole starch, amylose or amylopectin.
28235467	3	41	theme	amylopectin	351:361	arg1	structure					338:346	the structure	334:346	the structure of amylopectin	334:361	This modification determines the structure of amylopectin thus arranging the final composition of the starch granule.
28235467	2	42	theme	granule	221:227	arg1	assembly					229:236	starch granule assembly	214:236	starch granule assembly	214:236	This enzyme participates in starch granule assembly by the addition of α-1,6-glucan branches to the α-1,4-polyglucans.
28235467	5	43	dep	in	527:528	arg1	silico					530:535	silico	530:535	silico	530:535	Although in silico analysis suggested that this gene codes for a starch debranching enzyme, our biochemical studies support that this gene encodes a branching enzyme (BE).
28235467	1	44	theme	branching	117:125	arg1	protein					156:162	a highly conserved protein	137:162	a highly conserved protein from plants to algae	137:183	Starch branching enzyme is a highly conserved protein from plants to algae.
28235467	1	44	theme	branching	117:125	arg1	enzyme					127:132	Starch branching enzyme	110:132	Starch branching enzyme	110:132	Starch branching enzyme is a highly conserved protein from plants to algae.
28235467	0	45	theme	starch	47:52	arg1	enzyme					64:69	a novel starch branching enzyme	39:69	a novel starch branching enzyme from the picoalgae Ostreococcus tauri	39:107	Identification and characterization of a novel starch branching enzyme from the picoalgae Ostreococcus tauri.
28235467	6	46	dep	residues	837:844	arg1	64-403					846:851	64-403	846:851	64-403	846:851	The resulting 1058 amino acids protein has two in tandem carbohydrate binding domains (CBMs, from the CBM41 and CBM48 families) at the N-terminal (residues 64-403) followed by the C-terminal catalytic domain (residues 426-1058).
28235467	2	47	theme	starch	214:219	arg1	assembly					229:236	starch granule assembly	214:236	starch granule assembly	214:236	This enzyme participates in starch granule assembly by the addition of α-1,6-glucan branches to the α-1,4-polyglucans.
28235467	6	48	theme	tandem	740:745	arg1	domains					768:774	tandem carbohydrate binding domains	740:774	tandem carbohydrate binding domains (CBMs, from the CBM41 and CBM48 families)	740:816	The resulting 1058 amino acids protein has two in tandem carbohydrate binding domains (CBMs, from the CBM41 and CBM48 families) at the N-terminal (residues 64-403) followed by the C-terminal catalytic domain (residues 426-1058).
28235467	9	49	dep	functional	1261:1270	arg1	BE					1272:1273	BE	1272:1273	BE	1272:1273	Our results suggest that the Ot01g03030 gene codifies for a functional BE containing two CBMs from CBM41 and CBM48 families which are critical for enzyme function and regulation.
28235467	2	50	theme	α-1,6-glucan	257:268	arg1	branches					270:277	α-1,6-glucan branches	257:277	α-1,6-glucan branches	257:277	This enzyme participates in starch granule assembly by the addition of α-1,6-glucan branches to the α-1,4-polyglucans.
28235467	7	51	theme	the	931:933	arg1	isoforms					948:955	the BE truncated isoforms	931:955	the BE truncated isoforms	931:955	Analysis of the BE truncated isoforms show that the CBMs bind differentially to whole starch, amylose or amylopectin.
28235467	6	52	theme	carbohydrate	747:758	arg1	domains					768:774	tandem carbohydrate binding domains	740:774	tandem carbohydrate binding domains (CBMs, from the CBM41 and CBM48 families)	740:816	The resulting 1058 amino acids protein has two in tandem carbohydrate binding domains (CBMs, from the CBM41 and CBM48 families) at the N-terminal (residues 64-403) followed by the C-terminal catalytic domain (residues 426-1058).
28235467	0	53	from	tauri	103:107	arg1	characterization					19:34	characterization	19:34	characterization	19:34	Identification and characterization of a novel starch branching enzyme from the picoalgae Ostreococcus tauri.
28235467	0	53	from	tauri	103:107	arg1	Identification					0:13	Identification	0:13	Identification	0:13	Identification and characterization of a novel starch branching enzyme from the picoalgae Ostreococcus tauri.
28235467	0	53	from	tauri	103:107	arg1	enzyme					64:69	a novel starch branching enzyme	39:69	a novel starch branching enzyme from the picoalgae Ostreococcus tauri	39:107	Identification and characterization of a novel starch branching enzyme from the picoalgae Ostreococcus tauri.
28235467	7	54	theme	isoforms	948:955	arg1	Analysis					919:926	Analysis	919:926	Analysis of the BE truncated isoforms	919:955	Analysis of the BE truncated isoforms show that the CBMs bind differentially to whole starch, amylose or amylopectin.
28235467	6	55	contain	has	729:731	arg2	two					733:735	two	733:735	two	733:735	The resulting 1058 amino acids protein has two in tandem carbohydrate binding domains (CBMs, from the CBM41 and CBM48 families) at the N-terminal (residues 64-403) followed by the C-terminal catalytic domain (residues 426-1058).
28235467	6	55	contain	has	729:731	arg1	protein					721:727	The resulting 1058 amino acids protein	690:727	The resulting 1058 amino acids protein	690:727	The resulting 1058 amino acids protein has two in tandem carbohydrate binding domains (CBMs, from the CBM41 and CBM48 families) at the N-terminal (residues 64-403) followed by the C-terminal catalytic domain (residues 426-1058).
28235467	6	56	theme	CBM48	802:806	arg1	families					808:815	the CBM41 and CBM48 families	788:815	families	808:815	The resulting 1058 amino acids protein has two in tandem carbohydrate binding domains (CBMs, from the CBM41 and CBM48 families) at the N-terminal (residues 64-403) followed by the C-terminal catalytic domain (residues 426-1058).
28235467	7	57	dep	the	931:933	arg1	BE					935:936	BE	935:936	BE	935:936	Analysis of the BE truncated isoforms show that the CBMs bind differentially to whole starch, amylose or amylopectin.
28235467	0	58	theme	branching	54:62	arg1	enzyme					64:69	a novel starch branching enzyme	39:69	a novel starch branching enzyme from the picoalgae Ostreococcus tauri	39:107	Identification and characterization of a novel starch branching enzyme from the picoalgae Ostreococcus tauri.
28235467	9	59	contain	containing	1275:1284	arg1	functional					1261:1270	functional	1261:1270	functional	1261:1270	Our results suggest that the Ot01g03030 gene codifies for a functional BE containing two CBMs from CBM41 and CBM48 families which are critical for enzyme function and regulation.
28235467	9	59	contain	containing	1275:1284	arg2	CBMs					1290:1293	two CBMs	1286:1293	two CBMs	1286:1293	Our results suggest that the Ot01g03030 gene codifies for a functional BE containing two CBMs from CBM41 and CBM48 families which are critical for enzyme function and regulation.
28235467	1	60	from	plants	169:174	arg1	protein					156:162	a highly conserved protein	137:162	a highly conserved protein from plants to algae	137:183	Starch branching enzyme is a highly conserved protein from plants to algae.
28235467	1	60	from	plants	169:174	arg1	enzyme					127:132	Starch branching enzyme	110:132	Starch branching enzyme	110:132	Starch branching enzyme is a highly conserved protein from plants to algae.
28235467	1	61	theme	conserved	146:154	arg1	protein					156:162	a highly conserved protein	137:162	a highly conserved protein from plants to algae	137:183	Starch branching enzyme is a highly conserved protein from plants to algae.
28235467	1	61	theme	conserved	146:154	arg1	enzyme					127:132	Starch branching enzyme	110:132	Starch branching enzyme	110:132	Starch branching enzyme is a highly conserved protein from plants to algae.
28235467	0	62	theme	Ostreococcus	90:101	arg1	tauri					103:107	the picoalgae Ostreococcus tauri	76:107	the picoalgae Ostreococcus tauri	76:107	Identification and characterization of a novel starch branching enzyme from the picoalgae Ostreococcus tauri.
26428099	0	0	theme	phthalic	92:99	arg1	anhydride					101:109	phthalic anhydride	92:109	phthalic anhydride	92:109	Poly(butylene succinate-co-butylene adipate)/cellulose nanocrystal composites modified with phthalic anhydride.
26428099	3	1	theme	crystallization	719:733	arg1	curves					698:703	the curves	694:703	the curves of isothermal crystallization of the composites	694:751	The addition of phthalic anhydride could accelerate the crystallization of PBSA component as evidenced by the curves of isothermal crystallization of the composites, but had little effect on the crystalline polymorphs of PBSA component.
26428099	4	2	theme	anhydride	850:858	arg1	addition					829:836	The addition	825:836	The addition of phthalic anhydride	825:858	The addition of phthalic anhydride could strongly improve the hydrophobicity of the composites.
26428099	2	3	theme	phthalic	345:352	arg1	related					529:535	related	529:535	related	529:535	To improve the compatibility of poly(butylene succinate-co-butylene adipate) (PBSA)/CNC composites, phthalic anhydride was used as a compatilizer during melt mixing, leading to the significant improvement of the mechanical properties and thermal stability of the composites, which is related to the better dispersion of CNC in the composites.
26428099	2	3	theme	phthalic	345:352	arg1	compatilizer					378:389	a compatilizer	376:389	a compatilizer	376:389	To improve the compatibility of poly(butylene succinate-co-butylene adipate) (PBSA)/CNC composites, phthalic anhydride was used as a compatilizer during melt mixing, leading to the significant improvement of the mechanical properties and thermal stability of the composites, which is related to the better dispersion of CNC in the composites.
26428099	2	3	theme	phthalic	345:352	arg1	anhydride					354:362	phthalic anhydride	345:362	phthalic anhydride	345:362	To improve the compatibility of poly(butylene succinate-co-butylene adipate) (PBSA)/CNC composites, phthalic anhydride was used as a compatilizer during melt mixing, leading to the significant improvement of the mechanical properties and thermal stability of the composites, which is related to the better dispersion of CNC in the composites.
26428099	3	4	theme	PBSA	809:812	arg1	component					814:822	PBSA component	809:822	PBSA component	809:822	The addition of phthalic anhydride could accelerate the crystallization of PBSA component as evidenced by the curves of isothermal crystallization of the composites, but had little effect on the crystalline polymorphs of PBSA component.
26428099	2	5	theme	properties	468:477	arg1	improvement					438:448	the significant improvement	422:448	the significant improvement of the mechanical properties and thermal stability of the composites	422:517	To improve the compatibility of poly(butylene succinate-co-butylene adipate) (PBSA)/CNC composites, phthalic anhydride was used as a compatilizer during melt mixing, leading to the significant improvement of the mechanical properties and thermal stability of the composites, which is related to the better dispersion of CNC in the composites.
26428099	3	6	theme	component	668:676	arg1	crystallization					644:658	the crystallization	640:658	the crystallization of PBSA component	640:676	The addition of phthalic anhydride could accelerate the crystallization of PBSA component as evidenced by the curves of isothermal crystallization of the composites, but had little effect on the crystalline polymorphs of PBSA component.
26428099	4	7	theme	phthalic	841:848	arg1	anhydride					850:858	phthalic anhydride	841:858	phthalic anhydride	841:858	The addition of phthalic anhydride could strongly improve the hydrophobicity of the composites.
26428099	1	8	theme	cellulose	158:166	arg1	CNC					181:183	CNC	181:183	CNC	181:183	As a kind of biomass nanofiller for polymers, cellulose nanocrystal (CNC) has good mechanical properties and reinforcing capability.
26428099	1	8	theme	cellulose	158:166	arg1	nanocrystal					168:178	cellulose nanocrystal	158:178	cellulose nanocrystal (CNC)	158:184	As a kind of biomass nanofiller for polymers, cellulose nanocrystal (CNC) has good mechanical properties and reinforcing capability.
26428099	3	9	theme	component	814:822	arg1	polymorphs					795:804	the crystalline polymorphs	779:804	the crystalline polymorphs of PBSA component	779:822	The addition of phthalic anhydride could accelerate the crystallization of PBSA component as evidenced by the curves of isothermal crystallization of the composites, but had little effect on the crystalline polymorphs of PBSA component.
26428099	2	10	theme	butylene	282:289	arg1	adipate					313:319	butylene succinate-co-butylene adipate	282:319	butylene succinate-co-butylene adipate	282:319	To improve the compatibility of poly(butylene succinate-co-butylene adipate) (PBSA)/CNC composites, phthalic anhydride was used as a compatilizer during melt mixing, leading to the significant improvement of the mechanical properties and thermal stability of the composites, which is related to the better dispersion of CNC in the composites.
26428099	2	10	theme	butylene	282:289	arg1	poly					277:280	poly	277:280	poly(butylene succinate-co-butylene adipate) (PBSA)/CNC composites	277:342	To improve the compatibility of poly(butylene succinate-co-butylene adipate) (PBSA)/CNC composites, phthalic anhydride was used as a compatilizer during melt mixing, leading to the significant improvement of the mechanical properties and thermal stability of the composites, which is related to the better dispersion of CNC in the composites.
26428099	2	11	theme	composites	508:517	arg1	properties					468:477	mechanical properties	457:477	mechanical properties	457:477	To improve the compatibility of poly(butylene succinate-co-butylene adipate) (PBSA)/CNC composites, phthalic anhydride was used as a compatilizer during melt mixing, leading to the significant improvement of the mechanical properties and thermal stability of the composites, which is related to the better dispersion of CNC in the composites.
26428099	2	11	theme	composites	508:517	arg1	stability					491:499	thermal stability	483:499	thermal stability	483:499	To improve the compatibility of poly(butylene succinate-co-butylene adipate) (PBSA)/CNC composites, phthalic anhydride was used as a compatilizer during melt mixing, leading to the significant improvement of the mechanical properties and thermal stability of the composites, which is related to the better dispersion of CNC in the composites.
26428099	2	12	theme	CNC	565:567	arg1	dispersion					551:560	the better dispersion	540:560	the better dispersion of CNC in the composites	540:585	To improve the compatibility of poly(butylene succinate-co-butylene adipate) (PBSA)/CNC composites, phthalic anhydride was used as a compatilizer during melt mixing, leading to the significant improvement of the mechanical properties and thermal stability of the composites, which is related to the better dispersion of CNC in the composites.
26428099	2	13	from	dispersion	551:560	arg1	composites					576:585	the composites	572:585	the composites	572:585	To improve the compatibility of poly(butylene succinate-co-butylene adipate) (PBSA)/CNC composites, phthalic anhydride was used as a compatilizer during melt mixing, leading to the significant improvement of the mechanical properties and thermal stability of the composites, which is related to the better dispersion of CNC in the composites.
26428099	3	14	theme	isothermal	708:717	arg1	crystallization					719:733	isothermal crystallization	708:733	isothermal crystallization of the composites	708:751	The addition of phthalic anhydride could accelerate the crystallization of PBSA component as evidenced by the curves of isothermal crystallization of the composites, but had little effect on the crystalline polymorphs of PBSA component.
26428099	2	15	theme	poly	277:280	arg1	composites					333:342	poly(butylene succinate-co-butylene adipate) (PBSA)/CNC composites	277:342	poly(butylene succinate-co-butylene adipate) (PBSA)/CNC composites	277:342	To improve the compatibility of poly(butylene succinate-co-butylene adipate) (PBSA)/CNC composites, phthalic anhydride was used as a compatilizer during melt mixing, leading to the significant improvement of the mechanical properties and thermal stability of the composites, which is related to the better dispersion of CNC in the composites.
26428099	3	16	contain	had	758:760	arg2	effect					769:774	little effect	762:774	little effect	762:774	The addition of phthalic anhydride could accelerate the crystallization of PBSA component as evidenced by the curves of isothermal crystallization of the composites, but had little effect on the crystalline polymorphs of PBSA component.
26428099	3	16	contain	had	758:760	arg1	addition					592:599	The addition	588:599	The addition of phthalic anhydride	588:621	The addition of phthalic anhydride could accelerate the crystallization of PBSA component as evidenced by the curves of isothermal crystallization of the composites, but had little effect on the crystalline polymorphs of PBSA component.
26428099	2	17	theme	significant	426:436	arg1	improvement					438:448	the significant improvement	422:448	the significant improvement of the mechanical properties and thermal stability of the composites	422:517	To improve the compatibility of poly(butylene succinate-co-butylene adipate) (PBSA)/CNC composites, phthalic anhydride was used as a compatilizer during melt mixing, leading to the significant improvement of the mechanical properties and thermal stability of the composites, which is related to the better dispersion of CNC in the composites.
26428099	5	18	theme	good	925:928	arg1	properties					941:950	The good mechanical properties	921:950	The good mechanical properties	921:950	The good mechanical properties, fast crystallization and improved hydrophobicity of PBSA/CNC composites with phthalic anhydride are favor to their practical commercial utilization.
26428099	5	18	theme	good	925:928	arg1	favor					1053:1057	favor	1053:1057	favor	1053:1057	The good mechanical properties, fast crystallization and improved hydrophobicity of PBSA/CNC composites with phthalic anhydride are favor to their practical commercial utilization.
26428099	5	19	theme	practical	1068:1076	arg1	utilization					1089:1099	their practical commercial utilization	1062:1099	their practical commercial utilization	1062:1099	The good mechanical properties, fast crystallization and improved hydrophobicity of PBSA/CNC composites with phthalic anhydride are favor to their practical commercial utilization.
26428099	5	20	theme	mechanical	930:939	arg1	properties					941:950	The good mechanical properties	921:950	The good mechanical properties	921:950	The good mechanical properties, fast crystallization and improved hydrophobicity of PBSA/CNC composites with phthalic anhydride are favor to their practical commercial utilization.
26428099	5	20	theme	mechanical	930:939	arg1	favor					1053:1057	favor	1053:1057	favor	1053:1057	The good mechanical properties, fast crystallization and improved hydrophobicity of PBSA/CNC composites with phthalic anhydride are favor to their practical commercial utilization.
26428099	0	21	theme	succinate-co-butylene	14:34	arg1	Poly					0:3	Poly	0:3	Poly(butylene succinate-co-butylene adipate)	0:43	Poly(butylene succinate-co-butylene adipate)/cellulose nanocrystal composites modified with phthalic anhydride.
26428099	0	21	theme	succinate-co-butylene	14:34	arg1	adipate					36:42	butylene succinate-co-butylene adipate	5:42	butylene succinate-co-butylene adipate	5:42	Poly(butylene succinate-co-butylene adipate)/cellulose nanocrystal composites modified with phthalic anhydride.
26428099	2	22	from	CNC	565:567	arg1	composites					576:585	the composites	572:585	the composites	572:585	To improve the compatibility of poly(butylene succinate-co-butylene adipate) (PBSA)/CNC composites, phthalic anhydride was used as a compatilizer during melt mixing, leading to the significant improvement of the mechanical properties and thermal stability of the composites, which is related to the better dispersion of CNC in the composites.
26428099	4	23	theme	composites	909:918	arg1	hydrophobicity					887:900	the hydrophobicity	883:900	the hydrophobicity of the composites	883:918	The addition of phthalic anhydride could strongly improve the hydrophobicity of the composites.
26428099	0	24	theme	butylene	5:12	arg1	Poly					0:3	Poly	0:3	Poly(butylene succinate-co-butylene adipate)	0:43	Poly(butylene succinate-co-butylene adipate)/cellulose nanocrystal composites modified with phthalic anhydride.
26428099	0	24	theme	butylene	5:12	arg1	adipate					36:42	butylene succinate-co-butylene adipate	5:42	butylene succinate-co-butylene adipate	5:42	Poly(butylene succinate-co-butylene adipate)/cellulose nanocrystal composites modified with phthalic anhydride.
26428099	2	25	from	composites	576:585	arg1	dispersion					551:560	the better dispersion	540:560	the better dispersion of CNC in the composites	540:585	To improve the compatibility of poly(butylene succinate-co-butylene adipate) (PBSA)/CNC composites, phthalic anhydride was used as a compatilizer during melt mixing, leading to the significant improvement of the mechanical properties and thermal stability of the composites, which is related to the better dispersion of CNC in the composites.
26428099	5	26	theme	commercial	1078:1087	arg1	utilization					1089:1099	their practical commercial utilization	1062:1099	their practical commercial utilization	1062:1099	The good mechanical properties, fast crystallization and improved hydrophobicity of PBSA/CNC composites with phthalic anhydride are favor to their practical commercial utilization.
26428099	2	27	theme	succinate-co-butylene	291:311	arg1	adipate					313:319	butylene succinate-co-butylene adipate	282:319	butylene succinate-co-butylene adipate	282:319	To improve the compatibility of poly(butylene succinate-co-butylene adipate) (PBSA)/CNC composites, phthalic anhydride was used as a compatilizer during melt mixing, leading to the significant improvement of the mechanical properties and thermal stability of the composites, which is related to the better dispersion of CNC in the composites.
26428099	2	27	theme	succinate-co-butylene	291:311	arg1	poly					277:280	poly	277:280	poly(butylene succinate-co-butylene adipate) (PBSA)/CNC composites	277:342	To improve the compatibility of poly(butylene succinate-co-butylene adipate) (PBSA)/CNC composites, phthalic anhydride was used as a compatilizer during melt mixing, leading to the significant improvement of the mechanical properties and thermal stability of the composites, which is related to the better dispersion of CNC in the composites.
26428099	1	28	theme	good	190:193	arg1	kind					117:120	a kind	115:120	a kind of biomass nanofiller for polymers	115:155	As a kind of biomass nanofiller for polymers, cellulose nanocrystal (CNC) has good mechanical properties and reinforcing capability.
26428099	1	28	theme	good	190:193	arg1	properties					206:215	good mechanical properties	190:215	good mechanical properties	190:215	As a kind of biomass nanofiller for polymers, cellulose nanocrystal (CNC) has good mechanical properties and reinforcing capability.
26428099	2	29	theme	mechanical	457:466	arg1	properties					468:477	mechanical properties	457:477	mechanical properties	457:477	To improve the compatibility of poly(butylene succinate-co-butylene adipate) (PBSA)/CNC composites, phthalic anhydride was used as a compatilizer during melt mixing, leading to the significant improvement of the mechanical properties and thermal stability of the composites, which is related to the better dispersion of CNC in the composites.
26428099	1	30	theme	mechanical	195:204	arg1	kind					117:120	a kind	115:120	a kind of biomass nanofiller for polymers	115:155	As a kind of biomass nanofiller for polymers, cellulose nanocrystal (CNC) has good mechanical properties and reinforcing capability.
26428099	1	30	theme	mechanical	195:204	arg1	properties					206:215	good mechanical properties	190:215	good mechanical properties	190:215	As a kind of biomass nanofiller for polymers, cellulose nanocrystal (CNC) has good mechanical properties and reinforcing capability.
26428099	2	31	theme	melt	398:401	arg1	mixing					403:408	melt mixing	398:408	melt mixing	398:408	To improve the compatibility of poly(butylene succinate-co-butylene adipate) (PBSA)/CNC composites, phthalic anhydride was used as a compatilizer during melt mixing, leading to the significant improvement of the mechanical properties and thermal stability of the composites, which is related to the better dispersion of CNC in the composites.
26428099	3	32	theme	phthalic	604:611	arg1	anhydride					613:621	phthalic anhydride	604:621	phthalic anhydride	604:621	The addition of phthalic anhydride could accelerate the crystallization of PBSA component as evidenced by the curves of isothermal crystallization of the composites, but had little effect on the crystalline polymorphs of PBSA component.
26428099	5	33	theme	phthalic	1030:1037	arg1	anhydride					1039:1047	phthalic anhydride	1030:1047	phthalic anhydride	1030:1047	The good mechanical properties, fast crystallization and improved hydrophobicity of PBSA/CNC composites with phthalic anhydride are favor to their practical commercial utilization.
26428099	0	34	theme	nanocrystal	55:65	arg1	composites					67:76	nanocrystal composites	55:76	nanocrystal composites modified with phthalic anhydride	55:109	Poly(butylene succinate-co-butylene adipate)/cellulose nanocrystal composites modified with phthalic anhydride.
26428099	3	35	theme	little	762:767	arg1	effect					769:774	little effect	762:774	little effect	762:774	The addition of phthalic anhydride could accelerate the crystallization of PBSA component as evidenced by the curves of isothermal crystallization of the composites, but had little effect on the crystalline polymorphs of PBSA component.
26428099	3	36	theme	composites	742:751	arg1	crystallization					719:733	isothermal crystallization	708:733	isothermal crystallization of the composites	708:751	The addition of phthalic anhydride could accelerate the crystallization of PBSA component as evidenced by the curves of isothermal crystallization of the composites, but had little effect on the crystalline polymorphs of PBSA component.
26428099	2	37	theme	composites	333:342	arg1	compatibility					260:272	the compatibility	256:272	the compatibility of poly(butylene succinate-co-butylene adipate) (PBSA)/CNC composites	256:342	To improve the compatibility of poly(butylene succinate-co-butylene adipate) (PBSA)/CNC composites, phthalic anhydride was used as a compatilizer during melt mixing, leading to the significant improvement of the mechanical properties and thermal stability of the composites, which is related to the better dispersion of CNC in the composites.
26428099	2	38	dep	properties	468:477	arg1	the					453:455	the	453:455	the	453:455	To improve the compatibility of poly(butylene succinate-co-butylene adipate) (PBSA)/CNC composites, phthalic anhydride was used as a compatilizer during melt mixing, leading to the significant improvement of the mechanical properties and thermal stability of the composites, which is related to the better dispersion of CNC in the composites.
26428099	3	39	theme	anhydride	613:621	arg1	addition					592:599	The addition	588:599	The addition of phthalic anhydride	588:621	The addition of phthalic anhydride could accelerate the crystallization of PBSA component as evidenced by the curves of isothermal crystallization of the composites, but had little effect on the crystalline polymorphs of PBSA component.
26428099	2	40	theme	/CNC	328:331	arg1	composites					333:342	poly(butylene succinate-co-butylene adipate) (PBSA)/CNC composites	277:342	poly(butylene succinate-co-butylene adipate) (PBSA)/CNC composites	277:342	To improve the compatibility of poly(butylene succinate-co-butylene adipate) (PBSA)/CNC composites, phthalic anhydride was used as a compatilizer during melt mixing, leading to the significant improvement of the mechanical properties and thermal stability of the composites, which is related to the better dispersion of CNC in the composites.
26428099	5	41	with	composites	1014:1023	arg1	anhydride					1039:1047	phthalic anhydride	1030:1047	phthalic anhydride	1030:1047	The good mechanical properties, fast crystallization and improved hydrophobicity of PBSA/CNC composites with phthalic anhydride are favor to their practical commercial utilization.
26428099	2	42	theme	better	544:549	arg1	dispersion					551:560	the better dispersion	540:560	the better dispersion of CNC in the composites	540:585	To improve the compatibility of poly(butylene succinate-co-butylene adipate) (PBSA)/CNC composites, phthalic anhydride was used as a compatilizer during melt mixing, leading to the significant improvement of the mechanical properties and thermal stability of the composites, which is related to the better dispersion of CNC in the composites.
26428099	3	43	theme	PBSA	663:666	arg1	component					668:676	PBSA component	663:676	PBSA component	663:676	The addition of phthalic anhydride could accelerate the crystallization of PBSA component as evidenced by the curves of isothermal crystallization of the composites, but had little effect on the crystalline polymorphs of PBSA component.
26428099	1	44	theme	biomass	125:131	arg1	nanofiller					133:142	biomass nanofiller	125:142	biomass nanofiller for polymers	125:155	As a kind of biomass nanofiller for polymers, cellulose nanocrystal (CNC) has good mechanical properties and reinforcing capability.
26428099	5	45	theme	improved	978:985	arg1	hydrophobicity					987:1000	improved hydrophobicity	978:1000	improved hydrophobicity	978:1000	The good mechanical properties, fast crystallization and improved hydrophobicity of PBSA/CNC composites with phthalic anhydride are favor to their practical commercial utilization.
26428099	5	46	theme	fast	953:956	arg1	crystallization					958:972	fast crystallization	953:972	fast crystallization	953:972	The good mechanical properties, fast crystallization and improved hydrophobicity of PBSA/CNC composites with phthalic anhydride are favor to their practical commercial utilization.
26428099	2	47	used	used	368:371	arg2	anhydride					354:362	phthalic anhydride	345:362	phthalic anhydride	345:362	To improve the compatibility of poly(butylene succinate-co-butylene adipate) (PBSA)/CNC composites, phthalic anhydride was used as a compatilizer during melt mixing, leading to the significant improvement of the mechanical properties and thermal stability of the composites, which is related to the better dispersion of CNC in the composites.
26428099	2	47	used	used	368:371	arg2	compatilizer					378:389	a compatilizer	376:389	a compatilizer	376:389	To improve the compatibility of poly(butylene succinate-co-butylene adipate) (PBSA)/CNC composites, phthalic anhydride was used as a compatilizer during melt mixing, leading to the significant improvement of the mechanical properties and thermal stability of the composites, which is related to the better dispersion of CNC in the composites.
26428099	2	47	used	used	368:371	arg2	related					529:535	related	529:535	related	529:535	To improve the compatibility of poly(butylene succinate-co-butylene adipate) (PBSA)/CNC composites, phthalic anhydride was used as a compatilizer during melt mixing, leading to the significant improvement of the mechanical properties and thermal stability of the composites, which is related to the better dispersion of CNC in the composites.
26428099	1	48	theme	nanofiller	133:142	arg1	kind					117:120	a kind	115:120	a kind of biomass nanofiller for polymers	115:155	As a kind of biomass nanofiller for polymers, cellulose nanocrystal (CNC) has good mechanical properties and reinforcing capability.
26428099	1	48	theme	nanofiller	133:142	arg1	properties					206:215	good mechanical properties	190:215	good mechanical properties	190:215	As a kind of biomass nanofiller for polymers, cellulose nanocrystal (CNC) has good mechanical properties and reinforcing capability.
26428099	3	49	theme	crystalline	783:793	arg1	polymorphs					795:804	the crystalline polymorphs	779:804	the crystalline polymorphs of PBSA component	779:822	The addition of phthalic anhydride could accelerate the crystallization of PBSA component as evidenced by the curves of isothermal crystallization of the composites, but had little effect on the crystalline polymorphs of PBSA component.
26428099	2	50	theme	stability	491:499	arg1	improvement					438:448	the significant improvement	422:448	the significant improvement of the mechanical properties and thermal stability of the composites	422:517	To improve the compatibility of poly(butylene succinate-co-butylene adipate) (PBSA)/CNC composites, phthalic anhydride was used as a compatilizer during melt mixing, leading to the significant improvement of the mechanical properties and thermal stability of the composites, which is related to the better dispersion of CNC in the composites.
26428099	1	51	contain	has	186:188	arg2	properties					206:215	good mechanical properties	190:215	good mechanical properties	190:215	As a kind of biomass nanofiller for polymers, cellulose nanocrystal (CNC) has good mechanical properties and reinforcing capability.
26428099	1	51	contain	has	186:188	arg2	kind					117:120	a kind	115:120	a kind of biomass nanofiller for polymers	115:155	As a kind of biomass nanofiller for polymers, cellulose nanocrystal (CNC) has good mechanical properties and reinforcing capability.
26428099	1	51	contain	has	186:188	arg1	nanocrystal					168:178	cellulose nanocrystal	158:178	cellulose nanocrystal (CNC)	158:184	As a kind of biomass nanofiller for polymers, cellulose nanocrystal (CNC) has good mechanical properties and reinforcing capability.
26428099	1	51	contain	has	186:188	arg1	CNC					181:183	CNC	181:183	CNC	181:183	As a kind of biomass nanofiller for polymers, cellulose nanocrystal (CNC) has good mechanical properties and reinforcing capability.
26428099	5	52	theme	PBSA/CNC	1005:1012	arg1	composites					1014:1023	PBSA/CNC composites	1005:1023	PBSA/CNC composites with phthalic anhydride	1005:1047	The good mechanical properties, fast crystallization and improved hydrophobicity of PBSA/CNC composites with phthalic anhydride are favor to their practical commercial utilization.
26428099	2	53	theme	thermal	483:489	arg1	stability					491:499	thermal stability	483:499	thermal stability	483:499	To improve the compatibility of poly(butylene succinate-co-butylene adipate) (PBSA)/CNC composites, phthalic anhydride was used as a compatilizer during melt mixing, leading to the significant improvement of the mechanical properties and thermal stability of the composites, which is related to the better dispersion of CNC in the composites.
26428099	5	54	theme	composites	1014:1023	arg1	properties					941:950	The good mechanical properties	921:950	The good mechanical properties	921:950	The good mechanical properties, fast crystallization and improved hydrophobicity of PBSA/CNC composites with phthalic anhydride are favor to their practical commercial utilization.
26428099	5	54	theme	composites	1014:1023	arg1	crystallization					958:972	fast crystallization	953:972	fast crystallization	953:972	The good mechanical properties, fast crystallization and improved hydrophobicity of PBSA/CNC composites with phthalic anhydride are favor to their practical commercial utilization.
26428099	5	54	theme	composites	1014:1023	arg1	hydrophobicity					987:1000	improved hydrophobicity	978:1000	improved hydrophobicity	978:1000	The good mechanical properties, fast crystallization and improved hydrophobicity of PBSA/CNC composites with phthalic anhydride are favor to their practical commercial utilization.
26428099	5	54	theme	composites	1014:1023	arg1	favor					1053:1057	favor	1053:1057	favor	1053:1057	The good mechanical properties, fast crystallization and improved hydrophobicity of PBSA/CNC composites with phthalic anhydride are favor to their practical commercial utilization.
23913523	7	0	theme	dried	1094:1098	arg1	materials					1147:1155	high-DF materials	1139:1155	high-DF materials with a high proportion of soluble DF (∼20% of total DF) and considerable antioxidant capacity	1139:1249	The DF content changed in the dried residues, which could be considered as high-DF materials with a high proportion of soluble DF (∼20% of total DF) and considerable antioxidant capacity.
23913523	7	0	theme	dried	1094:1098	arg1	residues					1100:1107	the dried residues	1090:1107	the dried residues	1090:1107	The DF content changed in the dried residues, which could be considered as high-DF materials with a high proportion of soluble DF (∼20% of total DF) and considerable antioxidant capacity.
23913523	2	1	theme	food	313:316	arg1	industry					318:325	the food industry	309:325	the food industry	309:325	The market in this field is competitive and the search for new types of quality ingredients for the food industry is intensifying.
23913523	8	2	theme	antioxidant	1290:1300	arg1	source					1305:1310	an antioxidant DF source	1287:1310	an antioxidant DF source	1287:1310	These by-products could be used as an antioxidant DF source.
23913523	8	2	theme	antioxidant	1290:1300	arg1	by-products					1258:1268	These by-products	1252:1268	These by-products	1252:1268	These by-products could be used as an antioxidant DF source.
23913523	0	3	theme	polyphenols	83:93	arg1	source					73:78	a source	71:78	a source of polyphenols and dietary fiber	71:111	By-product from decoction process of Hibiscus sabdariffa L. calyces as a source of polyphenols and dietary fiber.
23913523	4	4	theme	decoction	775:783	arg1	residues					785:792	the decoction residues	771:792	the decoction residues	771:792	RESULTS Some calyx components were partially transferred to the beverage during the decoction process, while most were retained in the decoction residues.
23913523	6	5	theme	extractable	1039:1049	arg1	polyphenols					1051:1061	some extractable polyphenols	1034:1061	some extractable polyphenols	1034:1061	CONCLUSION The decoction process extracted some soluble carbohydrates, ash and some extractable polyphenols.
23913523	6	5	theme	extractable	1039:1049	arg1	carbohydrates					1011:1023	some soluble carbohydrates	998:1023	some soluble carbohydrates	998:1023	CONCLUSION The decoction process extracted some soluble carbohydrates, ash and some extractable polyphenols.
23913523	3	6	theme	sabdariffa	505:514	arg1	China					542:546	China'	542:547	China'	542:547	The aim of this study was to evaluate the composition and antioxidant activity of by-products generated during the decoction of calyces of four Mexican Hibiscus sabdariffa L. cultivars ('Criolla', 'China', 'Rosalis' and 'Tecoanapa') in order to assess them as a source of functional ingredients.
23913523	3	6	theme	sabdariffa	505:514	arg1	Tecoanapa					565:573	'Tecoanapa'	564:574	'Tecoanapa'	564:574	The aim of this study was to evaluate the composition and antioxidant activity of by-products generated during the decoction of calyces of four Mexican Hibiscus sabdariffa L. cultivars ('Criolla', 'China', 'Rosalis' and 'Tecoanapa') in order to assess them as a source of functional ingredients.
23913523	3	6	theme	sabdariffa	505:514	arg1	cultivars					519:527	Hibiscus sabdariffa L. cultivars	496:527	Hibiscus sabdariffa L. cultivars ('Criolla', 'China', 'Rosalis' and 'Tecoanapa') in order	496:584	The aim of this study was to evaluate the composition and antioxidant activity of by-products generated during the decoction of calyces of four Mexican Hibiscus sabdariffa L. cultivars ('Criolla', 'China', 'Rosalis' and 'Tecoanapa') in order to assess them as a source of functional ingredients.
23913523	3	6	theme	sabdariffa	505:514	arg1	Rosalis					551:557	'Rosalis'	550:558	'Rosalis'	550:558	The aim of this study was to evaluate the composition and antioxidant activity of by-products generated during the decoction of calyces of four Mexican Hibiscus sabdariffa L. cultivars ('Criolla', 'China', 'Rosalis' and 'Tecoanapa') in order to assess them as a source of functional ingredients.
23913523	5	7	theme	DF	843:844	arg1	by-products					801:811	These by-products	795:811	These by-products	795:811	These by-products proved to be a good source of DF (407.4-457.0 g kg⁻¹ dry matter) and natural antioxidants (50.7-121.8 µmol Trolox equivalent g⁻¹ dry matter).
23913523	5	7	theme	DF	843:844	arg1	source					833:838	a good source	826:838	a good source of DF (407.4-457.0 g kg⁻¹ dry matter) and natural antioxidants (50.7-121.8 µmol Trolox equivalent g⁻¹ dry matter)	826:952	These by-products proved to be a good source of DF (407.4-457.0 g kg⁻¹ dry matter) and natural antioxidants (50.7-121.8 µmol Trolox equivalent g⁻¹ dry matter).
23913523	4	8	theme	calyx	653:657	arg1	components					659:668	Some calyx components	648:668	Some calyx components	648:668	RESULTS Some calyx components were partially transferred to the beverage during the decoction process, while most were retained in the decoction residues.
23913523	3	9	theme	L.	516:517	arg1	China					542:546	China'	542:547	China'	542:547	The aim of this study was to evaluate the composition and antioxidant activity of by-products generated during the decoction of calyces of four Mexican Hibiscus sabdariffa L. cultivars ('Criolla', 'China', 'Rosalis' and 'Tecoanapa') in order to assess them as a source of functional ingredients.
23913523	3	9	theme	L.	516:517	arg1	Tecoanapa					565:573	'Tecoanapa'	564:574	'Tecoanapa'	564:574	The aim of this study was to evaluate the composition and antioxidant activity of by-products generated during the decoction of calyces of four Mexican Hibiscus sabdariffa L. cultivars ('Criolla', 'China', 'Rosalis' and 'Tecoanapa') in order to assess them as a source of functional ingredients.
23913523	3	9	theme	L.	516:517	arg1	cultivars					519:527	Hibiscus sabdariffa L. cultivars	496:527	Hibiscus sabdariffa L. cultivars ('Criolla', 'China', 'Rosalis' and 'Tecoanapa') in order	496:584	The aim of this study was to evaluate the composition and antioxidant activity of by-products generated during the decoction of calyces of four Mexican Hibiscus sabdariffa L. cultivars ('Criolla', 'China', 'Rosalis' and 'Tecoanapa') in order to assess them as a source of functional ingredients.
23913523	3	9	theme	L.	516:517	arg1	Rosalis					551:557	'Rosalis'	550:558	'Rosalis'	550:558	The aim of this study was to evaluate the composition and antioxidant activity of by-products generated during the decoction of calyces of four Mexican Hibiscus sabdariffa L. cultivars ('Criolla', 'China', 'Rosalis' and 'Tecoanapa') in order to assess them as a source of functional ingredients.
23913523	1	10	theme	Dietary	125:131	arg1	compounds					160:168	antioxidant compounds	148:168	antioxidant compounds	148:168	BACKGROUND Dietary fiber (DF) and antioxidant compounds are widely used as functional ingredients.
23913523	1	10	theme	Dietary	125:131	arg1	ingredients					200:210	functional ingredients	189:210	functional ingredients	189:210	BACKGROUND Dietary fiber (DF) and antioxidant compounds are widely used as functional ingredients.
23913523	1	10	theme	Dietary	125:131	arg1	DF					140:141	DF	140:141	DF	140:141	BACKGROUND Dietary fiber (DF) and antioxidant compounds are widely used as functional ingredients.
23913523	1	10	theme	Dietary	125:131	arg1	fiber					133:137	BACKGROUND Dietary fiber	114:137	BACKGROUND Dietary fiber (DF)	114:142	BACKGROUND Dietary fiber (DF) and antioxidant compounds are widely used as functional ingredients.
23913523	0	11	theme	dietary	99:105	arg1	fiber					107:111	dietary fiber	99:111	dietary fiber	99:111	By-product from decoction process of Hibiscus sabdariffa L. calyces as a source of polyphenols and dietary fiber.
23913523	3	12	theme	functional	616:625	arg1	ingredients					627:637	functional ingredients	616:637	functional ingredients	616:637	The aim of this study was to evaluate the composition and antioxidant activity of by-products generated during the decoction of calyces of four Mexican Hibiscus sabdariffa L. cultivars ('Criolla', 'China', 'Rosalis' and 'Tecoanapa') in order to assess them as a source of functional ingredients.
23913523	3	13	theme	Mexican	488:494	arg1	calyces					472:478	calyces	472:478	calyces of four Mexican Hibiscus sabdariffa L. cultivars ('Criolla', 'China', 'Rosalis' and 'Tecoanapa') in order to assess them as a source of functional ingredients	472:637	The aim of this study was to evaluate the composition and antioxidant activity of by-products generated during the decoction of calyces of four Mexican Hibiscus sabdariffa L. cultivars ('Criolla', 'China', 'Rosalis' and 'Tecoanapa') in order to assess them as a source of functional ingredients.
23913523	3	14	theme	study	360:364	arg1	aim					348:350	The aim	344:350	The aim of this study	344:364	The aim of this study was to evaluate the composition and antioxidant activity of by-products generated during the decoction of calyces of four Mexican Hibiscus sabdariffa L. cultivars ('Criolla', 'China', 'Rosalis' and 'Tecoanapa') in order to assess them as a source of functional ingredients.
23913523	3	15	theme	Hibiscus	496:503	arg1	China					542:546	China'	542:547	China'	542:547	The aim of this study was to evaluate the composition and antioxidant activity of by-products generated during the decoction of calyces of four Mexican Hibiscus sabdariffa L. cultivars ('Criolla', 'China', 'Rosalis' and 'Tecoanapa') in order to assess them as a source of functional ingredients.
23913523	3	15	theme	Hibiscus	496:503	arg1	Tecoanapa					565:573	'Tecoanapa'	564:574	'Tecoanapa'	564:574	The aim of this study was to evaluate the composition and antioxidant activity of by-products generated during the decoction of calyces of four Mexican Hibiscus sabdariffa L. cultivars ('Criolla', 'China', 'Rosalis' and 'Tecoanapa') in order to assess them as a source of functional ingredients.
23913523	3	15	theme	Hibiscus	496:503	arg1	cultivars					519:527	Hibiscus sabdariffa L. cultivars	496:527	Hibiscus sabdariffa L. cultivars ('Criolla', 'China', 'Rosalis' and 'Tecoanapa') in order	496:584	The aim of this study was to evaluate the composition and antioxidant activity of by-products generated during the decoction of calyces of four Mexican Hibiscus sabdariffa L. cultivars ('Criolla', 'China', 'Rosalis' and 'Tecoanapa') in order to assess them as a source of functional ingredients.
23913523	3	15	theme	Hibiscus	496:503	arg1	Rosalis					551:557	'Rosalis'	550:558	'Rosalis'	550:558	The aim of this study was to evaluate the composition and antioxidant activity of by-products generated during the decoction of calyces of four Mexican Hibiscus sabdariffa L. cultivars ('Criolla', 'China', 'Rosalis' and 'Tecoanapa') in order to assess them as a source of functional ingredients.
23913523	2	16	from	market	217:222	arg1	field					232:236	this field	227:236	this field	227:236	The market in this field is competitive and the search for new types of quality ingredients for the food industry is intensifying.
23913523	1	17	theme	antioxidant	148:158	arg1	compounds					160:168	antioxidant compounds	148:168	antioxidant compounds	148:168	BACKGROUND Dietary fiber (DF) and antioxidant compounds are widely used as functional ingredients.
23913523	1	17	theme	antioxidant	148:158	arg1	ingredients					200:210	functional ingredients	189:210	functional ingredients	189:210	BACKGROUND Dietary fiber (DF) and antioxidant compounds are widely used as functional ingredients.
23913523	1	17	theme	antioxidant	148:158	arg1	fiber					133:137	BACKGROUND Dietary fiber	114:137	BACKGROUND Dietary fiber (DF)	114:142	BACKGROUND Dietary fiber (DF) and antioxidant compounds are widely used as functional ingredients.
23913523	7	18	with	materials	1147:1155	arg1	proportion					1169:1178	a high proportion	1162:1178	a high proportion of soluble DF (∼20% of total DF) and considerable antioxidant capacity	1162:1249	The DF content changed in the dried residues, which could be considered as high-DF materials with a high proportion of soluble DF (∼20% of total DF) and considerable antioxidant capacity.
23913523	5	19	theme	good	828:831	arg1	by-products					801:811	These by-products	795:811	These by-products	795:811	These by-products proved to be a good source of DF (407.4-457.0 g kg⁻¹ dry matter) and natural antioxidants (50.7-121.8 µmol Trolox equivalent g⁻¹ dry matter).
23913523	5	19	theme	good	828:831	arg1	source					833:838	a good source	826:838	a good source of DF (407.4-457.0 g kg⁻¹ dry matter) and natural antioxidants (50.7-121.8 µmol Trolox equivalent g⁻¹ dry matter)	826:952	These by-products proved to be a good source of DF (407.4-457.0 g kg⁻¹ dry matter) and natural antioxidants (50.7-121.8 µmol Trolox equivalent g⁻¹ dry matter).
23913523	7	20	theme	high-DF	1139:1145	arg1	residues					1100:1107	the dried residues	1090:1107	the dried residues	1090:1107	The DF content changed in the dried residues, which could be considered as high-DF materials with a high proportion of soluble DF (∼20% of total DF) and considerable antioxidant capacity.
23913523	7	20	theme	high-DF	1139:1145	arg1	materials					1147:1155	high-DF materials	1139:1155	high-DF materials with a high proportion of soluble DF (∼20% of total DF) and considerable antioxidant capacity	1139:1249	The DF content changed in the dried residues, which could be considered as high-DF materials with a high proportion of soluble DF (∼20% of total DF) and considerable antioxidant capacity.
23913523	7	21	theme	soluble	1183:1189	arg1	DF					1191:1192	soluble DF	1183:1192	soluble DF (∼20% of total DF)	1183:1211	The DF content changed in the dried residues, which could be considered as high-DF materials with a high proportion of soluble DF (∼20% of total DF) and considerable antioxidant capacity.
23913523	5	22	theme	g⁻¹	938:940	arg1	matter					946:951	50.7-121.8 µmol Trolox equivalent g⁻¹ dry matter	904:951	50.7-121.8 µmol Trolox equivalent g⁻¹ dry matter	904:951	These by-products proved to be a good source of DF (407.4-457.0 g kg⁻¹ dry matter) and natural antioxidants (50.7-121.8 µmol Trolox equivalent g⁻¹ dry matter).
23913523	5	22	theme	g⁻¹	938:940	arg1	antioxidants					890:901	natural antioxidants	882:901	natural antioxidants (50.7-121.8 µmol Trolox equivalent g⁻¹ dry matter)	882:952	These by-products proved to be a good source of DF (407.4-457.0 g kg⁻¹ dry matter) and natural antioxidants (50.7-121.8 µmol Trolox equivalent g⁻¹ dry matter).
23913523	7	23	theme	total	1203:1207	arg1	DF					1209:1210	total DF	1203:1210	total DF	1203:1210	The DF content changed in the dried residues, which could be considered as high-DF materials with a high proportion of soluble DF (∼20% of total DF) and considerable antioxidant capacity.
23913523	8	24	used	used	1279:1282	arg2	source					1305:1310	an antioxidant DF source	1287:1310	an antioxidant DF source	1287:1310	These by-products could be used as an antioxidant DF source.
23913523	8	24	used	used	1279:1282	arg2	by-products					1258:1268	These by-products	1252:1268	These by-products	1252:1268	These by-products could be used as an antioxidant DF source.
23913523	7	25	theme	DF	1191:1192	arg1	proportion					1169:1178	a high proportion	1162:1178	a high proportion of soluble DF (∼20% of total DF) and considerable antioxidant capacity	1162:1249	The DF content changed in the dried residues, which could be considered as high-DF materials with a high proportion of soluble DF (∼20% of total DF) and considerable antioxidant capacity.
23913523	2	26	theme	new	272:274	arg1	types					276:280	new types	272:280	new types of quality ingredients	272:303	The market in this field is competitive and the search for new types of quality ingredients for the food industry is intensifying.
23913523	7	27	theme	considerable	1217:1228	arg1	capacity					1242:1249	considerable antioxidant capacity	1217:1249	considerable antioxidant capacity	1217:1249	The DF content changed in the dried residues, which could be considered as high-DF materials with a high proportion of soluble DF (∼20% of total DF) and considerable antioxidant capacity.
23913523	0	28	theme	decoction	16:24	arg1	process					26:32	decoction process	16:32	decoction process of Hibiscus sabdariffa L. calyces as a source of polyphenols and dietary fiber	16:111	By-product from decoction process of Hibiscus sabdariffa L. calyces as a source of polyphenols and dietary fiber.
23913523	3	29	theme	ingredients	627:637	arg1	them					596:599	them	596:599	them	596:599	The aim of this study was to evaluate the composition and antioxidant activity of by-products generated during the decoction of calyces of four Mexican Hibiscus sabdariffa L. cultivars ('Criolla', 'China', 'Rosalis' and 'Tecoanapa') in order to assess them as a source of functional ingredients.
23913523	3	29	theme	ingredients	627:637	arg1	source					606:611	a source	604:611	a source of functional ingredients	604:637	The aim of this study was to evaluate the composition and antioxidant activity of by-products generated during the decoction of calyces of four Mexican Hibiscus sabdariffa L. cultivars ('Criolla', 'China', 'Rosalis' and 'Tecoanapa') in order to assess them as a source of functional ingredients.
23913523	0	30	theme	fiber	107:111	arg1	source					73:78	a source	71:78	a source of polyphenols and dietary fiber	71:111	By-product from decoction process of Hibiscus sabdariffa L. calyces as a source of polyphenols and dietary fiber.
23913523	5	31	theme	dry	942:944	arg1	matter					946:951	50.7-121.8 µmol Trolox equivalent g⁻¹ dry matter	904:951	50.7-121.8 µmol Trolox equivalent g⁻¹ dry matter	904:951	These by-products proved to be a good source of DF (407.4-457.0 g kg⁻¹ dry matter) and natural antioxidants (50.7-121.8 µmol Trolox equivalent g⁻¹ dry matter).
23913523	5	31	theme	dry	942:944	arg1	antioxidants					890:901	natural antioxidants	882:901	natural antioxidants (50.7-121.8 µmol Trolox equivalent g⁻¹ dry matter)	882:952	These by-products proved to be a good source of DF (407.4-457.0 g kg⁻¹ dry matter) and natural antioxidants (50.7-121.8 µmol Trolox equivalent g⁻¹ dry matter).
23913523	3	32	theme	calyces	472:478	arg1	decoction					459:467	the decoction	455:467	the decoction of calyces of four Mexican Hibiscus sabdariffa L. cultivars ('Criolla', 'China', 'Rosalis' and 'Tecoanapa') in order to assess them as a source of functional ingredients	455:637	The aim of this study was to evaluate the composition and antioxidant activity of by-products generated during the decoction of calyces of four Mexican Hibiscus sabdariffa L. cultivars ('Criolla', 'China', 'Rosalis' and 'Tecoanapa') in order to assess them as a source of functional ingredients.
23913523	3	33	theme	by-products	426:436	arg1	composition					386:396	the composition	382:396	the composition	382:396	The aim of this study was to evaluate the composition and antioxidant activity of by-products generated during the decoction of calyces of four Mexican Hibiscus sabdariffa L. cultivars ('Criolla', 'China', 'Rosalis' and 'Tecoanapa') in order to assess them as a source of functional ingredients.
23913523	3	33	theme	by-products	426:436	arg1	activity					414:421	antioxidant activity	402:421	antioxidant activity of by-products generated during the decoction of calyces of four Mexican Hibiscus sabdariffa L. cultivars ('Criolla', 'China', 'Rosalis' and 'Tecoanapa') in order to assess them as a source of functional ingredients	402:637	The aim of this study was to evaluate the composition and antioxidant activity of by-products generated during the decoction of calyces of four Mexican Hibiscus sabdariffa L. cultivars ('Criolla', 'China', 'Rosalis' and 'Tecoanapa') in order to assess them as a source of functional ingredients.
23913523	0	34	theme	Hibiscus	37:44	arg1	calyces					60:66	Hibiscus sabdariffa L. calyces	37:66	Hibiscus sabdariffa L. calyces as a source of polyphenols and dietary fiber	37:111	By-product from decoction process of Hibiscus sabdariffa L. calyces as a source of polyphenols and dietary fiber.
23913523	6	35	theme	soluble	1003:1009	arg1	polyphenols					1051:1061	some extractable polyphenols	1034:1061	some extractable polyphenols	1034:1061	CONCLUSION The decoction process extracted some soluble carbohydrates, ash and some extractable polyphenols.
23913523	6	35	theme	soluble	1003:1009	arg1	ash					1026:1028	ash	1026:1028	ash	1026:1028	CONCLUSION The decoction process extracted some soluble carbohydrates, ash and some extractable polyphenols.
23913523	6	35	theme	soluble	1003:1009	arg1	carbohydrates					1011:1023	some soluble carbohydrates	998:1023	some soluble carbohydrates	998:1023	CONCLUSION The decoction process extracted some soluble carbohydrates, ash and some extractable polyphenols.
23913523	7	36	theme	DF	1068:1069	arg1	content					1071:1077	The DF content	1064:1077	The DF content	1064:1077	The DF content changed in the dried residues, which could be considered as high-DF materials with a high proportion of soluble DF (∼20% of total DF) and considerable antioxidant capacity.
23913523	5	37	theme	natural	882:888	arg1	antioxidants					890:901	natural antioxidants	882:901	natural antioxidants (50.7-121.8 µmol Trolox equivalent g⁻¹ dry matter)	882:952	These by-products proved to be a good source of DF (407.4-457.0 g kg⁻¹ dry matter) and natural antioxidants (50.7-121.8 µmol Trolox equivalent g⁻¹ dry matter).
23913523	5	37	theme	natural	882:888	arg1	matter					946:951	50.7-121.8 µmol Trolox equivalent g⁻¹ dry matter	904:951	50.7-121.8 µmol Trolox equivalent g⁻¹ dry matter	904:951	These by-products proved to be a good source of DF (407.4-457.0 g kg⁻¹ dry matter) and natural antioxidants (50.7-121.8 µmol Trolox equivalent g⁻¹ dry matter).
23913523	7	38	theme	high	1164:1167	arg1	proportion					1169:1178	a high proportion	1162:1178	a high proportion of soluble DF (∼20% of total DF) and considerable antioxidant capacity	1162:1249	The DF content changed in the dried residues, which could be considered as high-DF materials with a high proportion of soluble DF (∼20% of total DF) and considerable antioxidant capacity.
23913523	3	39	theme	antioxidant	402:412	arg1	activity					414:421	antioxidant activity	402:421	antioxidant activity of by-products generated during the decoction of calyces of four Mexican Hibiscus sabdariffa L. cultivars ('Criolla', 'China', 'Rosalis' and 'Tecoanapa') in order to assess them as a source of functional ingredients	402:637	The aim of this study was to evaluate the composition and antioxidant activity of by-products generated during the decoction of calyces of four Mexican Hibiscus sabdariffa L. cultivars ('Criolla', 'China', 'Rosalis' and 'Tecoanapa') in order to assess them as a source of functional ingredients.
23913523	5	40	theme	antioxidants	890:901	arg1	by-products					801:811	These by-products	795:811	These by-products	795:811	These by-products proved to be a good source of DF (407.4-457.0 g kg⁻¹ dry matter) and natural antioxidants (50.7-121.8 µmol Trolox equivalent g⁻¹ dry matter).
23913523	5	40	theme	antioxidants	890:901	arg1	source					833:838	a good source	826:838	a good source of DF (407.4-457.0 g kg⁻¹ dry matter) and natural antioxidants (50.7-121.8 µmol Trolox equivalent g⁻¹ dry matter)	826:952	These by-products proved to be a good source of DF (407.4-457.0 g kg⁻¹ dry matter) and natural antioxidants (50.7-121.8 µmol Trolox equivalent g⁻¹ dry matter).
23913523	1	41	theme	functional	189:198	arg1	compounds					160:168	antioxidant compounds	148:168	antioxidant compounds	148:168	BACKGROUND Dietary fiber (DF) and antioxidant compounds are widely used as functional ingredients.
23913523	1	41	theme	functional	189:198	arg1	ingredients					200:210	functional ingredients	189:210	functional ingredients	189:210	BACKGROUND Dietary fiber (DF) and antioxidant compounds are widely used as functional ingredients.
23913523	1	41	theme	functional	189:198	arg1	fiber					133:137	BACKGROUND Dietary fiber	114:137	BACKGROUND Dietary fiber (DF)	114:142	BACKGROUND Dietary fiber (DF) and antioxidant compounds are widely used as functional ingredients.
23913523	0	42	theme	L.	57:58	arg1	calyces					60:66	Hibiscus sabdariffa L. calyces	37:66	Hibiscus sabdariffa L. calyces as a source of polyphenols and dietary fiber	37:111	By-product from decoction process of Hibiscus sabdariffa L. calyces as a source of polyphenols and dietary fiber.
23913523	8	43	theme	DF	1302:1303	arg1	source					1305:1310	an antioxidant DF source	1287:1310	an antioxidant DF source	1287:1310	These by-products could be used as an antioxidant DF source.
23913523	8	43	theme	DF	1302:1303	arg1	by-products					1258:1268	These by-products	1252:1268	These by-products	1252:1268	These by-products could be used as an antioxidant DF source.
23913523	1	44	used	used	181:184	arg2	ingredients					200:210	functional ingredients	189:210	functional ingredients	189:210	BACKGROUND Dietary fiber (DF) and antioxidant compounds are widely used as functional ingredients.
23913523	1	44	used	used	181:184	arg2	DF					140:141	DF	140:141	DF	140:141	BACKGROUND Dietary fiber (DF) and antioxidant compounds are widely used as functional ingredients.
23913523	1	44	used	used	181:184	arg2	fiber					133:137	BACKGROUND Dietary fiber	114:137	BACKGROUND Dietary fiber (DF)	114:142	BACKGROUND Dietary fiber (DF) and antioxidant compounds are widely used as functional ingredients.
23913523	1	44	used	used	181:184	arg2	compounds					160:168	antioxidant compounds	148:168	antioxidant compounds	148:168	BACKGROUND Dietary fiber (DF) and antioxidant compounds are widely used as functional ingredients.
23913523	0	45	theme	sabdariffa	46:55	arg1	calyces					60:66	Hibiscus sabdariffa L. calyces	37:66	Hibiscus sabdariffa L. calyces as a source of polyphenols and dietary fiber	37:111	By-product from decoction process of Hibiscus sabdariffa L. calyces as a source of polyphenols and dietary fiber.
23913523	5	46	theme	µmol	915:918	arg1	matter					946:951	50.7-121.8 µmol Trolox equivalent g⁻¹ dry matter	904:951	50.7-121.8 µmol Trolox equivalent g⁻¹ dry matter	904:951	These by-products proved to be a good source of DF (407.4-457.0 g kg⁻¹ dry matter) and natural antioxidants (50.7-121.8 µmol Trolox equivalent g⁻¹ dry matter).
23913523	5	46	theme	µmol	915:918	arg1	antioxidants					890:901	natural antioxidants	882:901	natural antioxidants (50.7-121.8 µmol Trolox equivalent g⁻¹ dry matter)	882:952	These by-products proved to be a good source of DF (407.4-457.0 g kg⁻¹ dry matter) and natural antioxidants (50.7-121.8 µmol Trolox equivalent g⁻¹ dry matter).
23913523	4	47	theme	decoction	724:732	arg1	process					734:740	the decoction process	720:740	the decoction process	720:740	RESULTS Some calyx components were partially transferred to the beverage during the decoction process, while most were retained in the decoction residues.
23913523	4	48	dep	RESULTS	640:646	arg1	transferred					685:695	transferred	685:695	transferred to the beverage during the decoction process	685:740	RESULTS Some calyx components were partially transferred to the beverage during the decoction process, while most were retained in the decoction residues.
23913523	5	49	theme	Trolox	920:925	arg1	matter					946:951	50.7-121.8 µmol Trolox equivalent g⁻¹ dry matter	904:951	50.7-121.8 µmol Trolox equivalent g⁻¹ dry matter	904:951	These by-products proved to be a good source of DF (407.4-457.0 g kg⁻¹ dry matter) and natural antioxidants (50.7-121.8 µmol Trolox equivalent g⁻¹ dry matter).
23913523	5	49	theme	Trolox	920:925	arg1	antioxidants					890:901	natural antioxidants	882:901	natural antioxidants (50.7-121.8 µmol Trolox equivalent g⁻¹ dry matter)	882:952	These by-products proved to be a good source of DF (407.4-457.0 g kg⁻¹ dry matter) and natural antioxidants (50.7-121.8 µmol Trolox equivalent g⁻¹ dry matter).
23913523	5	50	theme	dry	866:868	arg1	DF					843:844	DF	843:844	DF (407.4-457.0 g kg⁻¹ dry matter)	843:876	These by-products proved to be a good source of DF (407.4-457.0 g kg⁻¹ dry matter) and natural antioxidants (50.7-121.8 µmol Trolox equivalent g⁻¹ dry matter).
23913523	5	50	theme	dry	866:868	arg1	matter					870:875	407.4-457.0 g kg⁻¹ dry matter	847:875	407.4-457.0 g kg⁻¹ dry matter	847:875	These by-products proved to be a good source of DF (407.4-457.0 g kg⁻¹ dry matter) and natural antioxidants (50.7-121.8 µmol Trolox equivalent g⁻¹ dry matter).
23913523	7	51	theme	antioxidant	1230:1240	arg1	capacity					1242:1249	considerable antioxidant capacity	1217:1249	considerable antioxidant capacity	1217:1249	The DF content changed in the dried residues, which could be considered as high-DF materials with a high proportion of soluble DF (∼20% of total DF) and considerable antioxidant capacity.
23913523	1	52	theme	BACKGROUND	114:123	arg1	compounds					160:168	antioxidant compounds	148:168	antioxidant compounds	148:168	BACKGROUND Dietary fiber (DF) and antioxidant compounds are widely used as functional ingredients.
23913523	1	52	theme	BACKGROUND	114:123	arg1	ingredients					200:210	functional ingredients	189:210	functional ingredients	189:210	BACKGROUND Dietary fiber (DF) and antioxidant compounds are widely used as functional ingredients.
23913523	1	52	theme	BACKGROUND	114:123	arg1	DF					140:141	DF	140:141	DF	140:141	BACKGROUND Dietary fiber (DF) and antioxidant compounds are widely used as functional ingredients.
23913523	1	52	theme	BACKGROUND	114:123	arg1	fiber					133:137	BACKGROUND Dietary fiber	114:137	BACKGROUND Dietary fiber (DF)	114:142	BACKGROUND Dietary fiber (DF) and antioxidant compounds are widely used as functional ingredients.
23913523	7	53	theme	capacity	1242:1249	arg1	proportion					1169:1178	a high proportion	1162:1178	a high proportion of soluble DF (∼20% of total DF) and considerable antioxidant capacity	1162:1249	The DF content changed in the dried residues, which could be considered as high-DF materials with a high proportion of soluble DF (∼20% of total DF) and considerable antioxidant capacity.
23913523	5	54	theme	kg⁻¹	861:864	arg1	DF					843:844	DF	843:844	DF (407.4-457.0 g kg⁻¹ dry matter)	843:876	These by-products proved to be a good source of DF (407.4-457.0 g kg⁻¹ dry matter) and natural antioxidants (50.7-121.8 µmol Trolox equivalent g⁻¹ dry matter).
23913523	5	54	theme	kg⁻¹	861:864	arg1	matter					870:875	407.4-457.0 g kg⁻¹ dry matter	847:875	407.4-457.0 g kg⁻¹ dry matter	847:875	These by-products proved to be a good source of DF (407.4-457.0 g kg⁻¹ dry matter) and natural antioxidants (50.7-121.8 µmol Trolox equivalent g⁻¹ dry matter).
23913523	5	55	theme	equivalent	927:936	arg1	matter					946:951	50.7-121.8 µmol Trolox equivalent g⁻¹ dry matter	904:951	50.7-121.8 µmol Trolox equivalent g⁻¹ dry matter	904:951	These by-products proved to be a good source of DF (407.4-457.0 g kg⁻¹ dry matter) and natural antioxidants (50.7-121.8 µmol Trolox equivalent g⁻¹ dry matter).
23913523	5	55	theme	equivalent	927:936	arg1	antioxidants					890:901	natural antioxidants	882:901	natural antioxidants (50.7-121.8 µmol Trolox equivalent g⁻¹ dry matter)	882:952	These by-products proved to be a good source of DF (407.4-457.0 g kg⁻¹ dry matter) and natural antioxidants (50.7-121.8 µmol Trolox equivalent g⁻¹ dry matter).
23913523	7	56	theme	DF	1209:1210	arg1	%					1198:1198	∼20%	1195:1198	∼20% of total DF	1195:1210	The DF content changed in the dried residues, which could be considered as high-DF materials with a high proportion of soluble DF (∼20% of total DF) and considerable antioxidant capacity.
23913523	7	56	theme	DF	1209:1210	arg1	DF					1209:1210	total DF	1203:1210	total DF	1203:1210	The DF content changed in the dried residues, which could be considered as high-DF materials with a high proportion of soluble DF (∼20% of total DF) and considerable antioxidant capacity.
23913523	2	57	theme	quality	285:291	arg1	ingredients					293:303	quality ingredients	285:303	quality ingredients	285:303	The market in this field is competitive and the search for new types of quality ingredients for the food industry is intensifying.
23913523	2	58	theme	ingredients	293:303	arg1	types					276:280	new types	272:280	new types of quality ingredients	272:303	The market in this field is competitive and the search for new types of quality ingredients for the food industry is intensifying.
23913523	5	59	theme	g	859:859	arg1	DF					843:844	DF	843:844	DF (407.4-457.0 g kg⁻¹ dry matter)	843:876	These by-products proved to be a good source of DF (407.4-457.0 g kg⁻¹ dry matter) and natural antioxidants (50.7-121.8 µmol Trolox equivalent g⁻¹ dry matter).
23913523	5	59	theme	g	859:859	arg1	matter					870:875	407.4-457.0 g kg⁻¹ dry matter	847:875	407.4-457.0 g kg⁻¹ dry matter	847:875	These by-products proved to be a good source of DF (407.4-457.0 g kg⁻¹ dry matter) and natural antioxidants (50.7-121.8 µmol Trolox equivalent g⁻¹ dry matter).
23913523	3	60	dep	cultivars	519:527	arg1	cultivars					519:527	Hibiscus sabdariffa L. cultivars	496:527	Hibiscus sabdariffa L. cultivars ('Criolla', 'China', 'Rosalis' and 'Tecoanapa') in order	496:584	The aim of this study was to evaluate the composition and antioxidant activity of by-products generated during the decoction of calyces of four Mexican Hibiscus sabdariffa L. cultivars ('Criolla', 'China', 'Rosalis' and 'Tecoanapa') in order to assess them as a source of functional ingredients.
23913523	3	60	dep	cultivars	519:527	arg1	China					542:546	China'	542:547	China'	542:547	The aim of this study was to evaluate the composition and antioxidant activity of by-products generated during the decoction of calyces of four Mexican Hibiscus sabdariffa L. cultivars ('Criolla', 'China', 'Rosalis' and 'Tecoanapa') in order to assess them as a source of functional ingredients.
23913523	3	60	dep	cultivars	519:527	arg1	Tecoanapa					565:573	'Tecoanapa'	564:574	'Tecoanapa'	564:574	The aim of this study was to evaluate the composition and antioxidant activity of by-products generated during the decoction of calyces of four Mexican Hibiscus sabdariffa L. cultivars ('Criolla', 'China', 'Rosalis' and 'Tecoanapa') in order to assess them as a source of functional ingredients.
23913523	3	60	dep	cultivars	519:527	arg1	Criolla					531:537	Criolla	531:537	Criolla	531:537	The aim of this study was to evaluate the composition and antioxidant activity of by-products generated during the decoction of calyces of four Mexican Hibiscus sabdariffa L. cultivars ('Criolla', 'China', 'Rosalis' and 'Tecoanapa') in order to assess them as a source of functional ingredients.
23913523	3	60	dep	cultivars	519:527	arg1	Rosalis					551:557	'Rosalis'	550:558	'Rosalis'	550:558	The aim of this study was to evaluate the composition and antioxidant activity of by-products generated during the decoction of calyces of four Mexican Hibiscus sabdariffa L. cultivars ('Criolla', 'China', 'Rosalis' and 'Tecoanapa') in order to assess them as a source of functional ingredients.
23913523	7	61	dep	DF	1191:1192	arg1	%					1198:1198	∼20%	1195:1198	∼20% of total DF	1195:1210	The DF content changed in the dried residues, which could be considered as high-DF materials with a high proportion of soluble DF (∼20% of total DF) and considerable antioxidant capacity.
23913523	7	61	dep	DF	1191:1192	arg1	DF					1209:1210	total DF	1203:1210	total DF	1203:1210	The DF content changed in the dried residues, which could be considered as high-DF materials with a high proportion of soluble DF (∼20% of total DF) and considerable antioxidant capacity.
23913523	0	62	theme	calyces	60:66	arg1	process					26:32	decoction process	16:32	decoction process of Hibiscus sabdariffa L. calyces as a source of polyphenols and dietary fiber	16:111	By-product from decoction process of Hibiscus sabdariffa L. calyces as a source of polyphenols and dietary fiber.
23913523	0	63	from	process	26:32	arg1	By-product					0:9	By-product	0:9	By-product	0:9	By-product from decoction process of Hibiscus sabdariffa L. calyces as a source of polyphenols and dietary fiber.
23913523	6	64	theme	decoction	970:978	arg1	process					980:986	The decoction process	966:986	The decoction process extracted some soluble carbohydrates, ash and some extractable polyphenols	966:1061	CONCLUSION The decoction process extracted some soluble carbohydrates, ash and some extractable polyphenols.
26820354	9	0	theme	potential	1262:1270	arg1	source					1272:1277	a potential source	1260:1277	a potential source of immunoregulatory polysaccharide	1260:1312	Current study suggested that the lactic bacteria strain AR333 could be a potential source of immunoregulatory polysaccharide.
26820354	9	0	theme	potential	1262:1270	arg1	AR333					1245:1249	the lactic bacteria strain AR333	1218:1249	the lactic bacteria strain AR333	1218:1249	Current study suggested that the lactic bacteria strain AR333 could be a potential source of immunoregulatory polysaccharide.
26820354	6	1	theme	pure	935:938	arg1	polysaccharide					948:961	a pure neutral polysaccharide	933:961	a pure neutral polysaccharide with monosaccharide composition of galactose and glucose	933:1018	The results showed that EPS333 was a pure neutral polysaccharide with monosaccharide composition of galactose and glucose in a molar ratio of 6:5.
26820354	6	1	theme	pure	935:938	arg1	EPS333					922:927	EPS333	922:927	EPS333	922:927	The results showed that EPS333 was a pure neutral polysaccharide with monosaccharide composition of galactose and glucose in a molar ratio of 6:5.
26820354	5	2	theme	size	859:862	arg1	chromatography					874:887	high-performance size exclusion chromatography	842:887	high-performance size exclusion chromatography (HPSEC)	842:895	The further work tried to elucidate the structural features of EPS333 via FT-IR spectrum, high-performance anion exchange chromatography (HPAEC) and high-performance size exclusion chromatography (HPSEC).
26820354	5	2	theme	size	859:862	arg1	HPSEC					890:894	HPSEC	890:894	HPSEC	890:894	The further work tried to elucidate the structural features of EPS333 via FT-IR spectrum, high-performance anion exchange chromatography (HPAEC) and high-performance size exclusion chromatography (HPSEC).
26820354	2	3	theme	biochemical	380:390	arg1	characteristics					392:406	physiological and biochemical characteristics	362:406	physiological and biochemical characteristics	362:406	It was identified as Streptococcus thermophilus by morphological observation, physiological and biochemical characteristics, and 16S rDNA sequence determination.
26820354	5	4	theme	high-performance	783:798	arg1	chromatography					815:828	high-performance anion exchange chromatography	783:828	high-performance anion exchange chromatography (HPAEC)	783:836	The further work tried to elucidate the structural features of EPS333 via FT-IR spectrum, high-performance anion exchange chromatography (HPAEC) and high-performance size exclusion chromatography (HPSEC).
26820354	5	4	theme	high-performance	783:798	arg1	HPAEC					831:835	HPAEC	831:835	HPAEC	831:835	The further work tried to elucidate the structural features of EPS333 via FT-IR spectrum, high-performance anion exchange chromatography (HPAEC) and high-performance size exclusion chromatography (HPSEC).
26820354	1	5	theme	exopolysaccharides	166:183	arg1	high-production					147:161	high-production	147:161	high-production of exopolysaccharides (EPS)	147:189	A lactic acid bacteria strain AR333 with high-production of exopolysaccharides (EPS) was screened out from 350 bacteria strains isolated from naturally fermented dairy products.
26820354	1	6	theme	fermented	258:266	arg1	products					274:281	naturally fermented dairy products	248:281	naturally fermented dairy products	248:281	A lactic acid bacteria strain AR333 with high-production of exopolysaccharides (EPS) was screened out from 350 bacteria strains isolated from naturally fermented dairy products.
26820354	7	7	theme	acetyl	1065:1070	arg1	groups					1072:1077	acetyl groups	1065:1077	acetyl groups	1065:1077	A certain amount of acetyl groups might exist in EPS333 according to the FT-IR spectrum.
26820354	9	8	theme	immunoregulatory	1282:1297	arg1	polysaccharide					1299:1312	immunoregulatory polysaccharide	1282:1312	immunoregulatory polysaccharide	1282:1312	Current study suggested that the lactic bacteria strain AR333 could be a potential source of immunoregulatory polysaccharide.
26820354	7	9	theme	FT-IR	1118:1122	arg1	spectrum					1124:1131	the FT-IR spectrum	1114:1131	the FT-IR spectrum	1114:1131	A certain amount of acetyl groups might exist in EPS333 according to the FT-IR spectrum.
26820354	1	10	theme	dairy	268:272	arg1	products					274:281	naturally fermented dairy products	248:281	naturally fermented dairy products	248:281	A lactic acid bacteria strain AR333 with high-production of exopolysaccharides (EPS) was screened out from 350 bacteria strains isolated from naturally fermented dairy products.
26820354	9	11	theme	polysaccharide	1299:1312	arg1	source					1272:1277	a potential source	1260:1277	a potential source of immunoregulatory polysaccharide	1260:1312	Current study suggested that the lactic bacteria strain AR333 could be a potential source of immunoregulatory polysaccharide.
26820354	9	11	theme	polysaccharide	1299:1312	arg1	AR333					1245:1249	the lactic bacteria strain AR333	1218:1249	the lactic bacteria strain AR333	1218:1249	Current study suggested that the lactic bacteria strain AR333 could be a potential source of immunoregulatory polysaccharide.
26820354	3	12	theme	Sepharose	516:524	arg1	CL-6B					526:530	Sepharose CL-6B	516:530	Sepharose CL-6B	516:530	The EPS from AR333 were purified through DEAE-Sepharose Fast Flow and Sepharose CL-6B, and the purified fraction was designated as EPS333.
26820354	5	13	theme	EPS333	756:761	arg1	features					744:751	the structural features	729:751	the structural features of EPS333	729:761	The further work tried to elucidate the structural features of EPS333 via FT-IR spectrum, high-performance anion exchange chromatography (HPAEC) and high-performance size exclusion chromatography (HPSEC).
26820354	1	14	theme	acid	115:118	arg1	bacteria					120:127	lactic acid bacteria	108:127	A lactic acid bacteria strain AR333 with high-production of exopolysaccharides (EPS)	106:189	A lactic acid bacteria strain AR333 with high-production of exopolysaccharides (EPS) was screened out from 350 bacteria strains isolated from naturally fermented dairy products.
26820354	2	15	theme	sequence	422:429	arg1	determination					431:443	sequence determination	422:443	sequence determination	422:443	It was identified as Streptococcus thermophilus by morphological observation, physiological and biochemical characteristics, and 16S rDNA sequence determination.
26820354	4	16	dep	In	585:586	arg1	vitro					588:592	vitro	588:592	vitro	588:592	In vitro test showed that EPS333 could stimulate macrophage RAW 264.7 to release NO significantly (p<0.05).
26820354	1	17	with	AR333	136:140	arg1	high-production					147:161	high-production	147:161	high-production of exopolysaccharides (EPS)	147:189	A lactic acid bacteria strain AR333 with high-production of exopolysaccharides (EPS) was screened out from 350 bacteria strains isolated from naturally fermented dairy products.
26820354	9	18	theme	Current	1189:1195	arg1	study					1197:1201	Current study	1189:1201	Current study	1189:1201	Current study suggested that the lactic bacteria strain AR333 could be a potential source of immunoregulatory polysaccharide.
26820354	9	19	theme	bacteria	1229:1236	arg1	source					1272:1277	a potential source	1260:1277	a potential source of immunoregulatory polysaccharide	1260:1312	Current study suggested that the lactic bacteria strain AR333 could be a potential source of immunoregulatory polysaccharide.
26820354	9	19	theme	bacteria	1229:1236	arg1	AR333					1245:1249	the lactic bacteria strain AR333	1218:1249	the lactic bacteria strain AR333	1218:1249	Current study suggested that the lactic bacteria strain AR333 could be a potential source of immunoregulatory polysaccharide.
26820354	6	20	theme	glucose	1012:1018	arg1	composition					983:993	monosaccharide composition	968:993	monosaccharide composition of galactose and glucose	968:1018	The results showed that EPS333 was a pure neutral polysaccharide with monosaccharide composition of galactose and glucose in a molar ratio of 6:5.
26820354	2	21	dep	observation	349:359	arg1	determination					431:443	sequence determination	422:443	sequence determination	422:443	It was identified as Streptococcus thermophilus by morphological observation, physiological and biochemical characteristics, and 16S rDNA sequence determination.
26820354	0	22	from	strain	52:57	arg1	purification					71:82	purification	71:82	purification	71:82	Bioactive exopolysaccharides from a S. thermophilus strain: Screening, purification and characterization.
26820354	0	22	from	strain	52:57	arg1	Screening					60:68	Screening	60:68	Screening	60:68	Bioactive exopolysaccharides from a S. thermophilus strain: Screening, purification and characterization.
26820354	0	22	from	strain	52:57	arg1	exopolysaccharides					10:27	Bioactive exopolysaccharides	0:27	Bioactive exopolysaccharides from a S. thermophilus strain: Screening, purification and characterization.	0:104	Bioactive exopolysaccharides from a S. thermophilus strain: Screening, purification and characterization.
26820354	0	22	from	strain	52:57	arg1	characterization					88:103	characterization	88:103	characterization	88:103	Bioactive exopolysaccharides from a S. thermophilus strain: Screening, purification and characterization.
26820354	2	23	theme	16S	413:415	arg1	rDNA					417:420	16S rDNA	413:420	16S rDNA	413:420	It was identified as Streptococcus thermophilus by morphological observation, physiological and biochemical characteristics, and 16S rDNA sequence determination.
26820354	0	24	theme	Bioactive	0:8	arg1	purification					71:82	purification	71:82	purification	71:82	Bioactive exopolysaccharides from a S. thermophilus strain: Screening, purification and characterization.
26820354	0	24	theme	Bioactive	0:8	arg1	Screening					60:68	Screening	60:68	Screening	60:68	Bioactive exopolysaccharides from a S. thermophilus strain: Screening, purification and characterization.
26820354	0	24	theme	Bioactive	0:8	arg1	exopolysaccharides					10:27	Bioactive exopolysaccharides	0:27	Bioactive exopolysaccharides from a S. thermophilus strain: Screening, purification and characterization.	0:104	Bioactive exopolysaccharides from a S. thermophilus strain: Screening, purification and characterization.
26820354	0	24	theme	Bioactive	0:8	arg1	characterization					88:103	characterization	88:103	characterization	88:103	Bioactive exopolysaccharides from a S. thermophilus strain: Screening, purification and characterization.
26820354	4	25	theme	In	585:586	arg1	test					594:597	In vitro test	585:597	In vitro test	585:597	In vitro test showed that EPS333 could stimulate macrophage RAW 264.7 to release NO significantly (p<0.05).
26820354	6	26	with	polysaccharide	948:961	arg1	composition					983:993	monosaccharide composition	968:993	monosaccharide composition of galactose and glucose	968:1018	The results showed that EPS333 was a pure neutral polysaccharide with monosaccharide composition of galactose and glucose in a molar ratio of 6:5.
26820354	1	27	attach	isolated	234:241	arg2	strains					226:232	350 bacteria strains	213:232	350 bacteria strains isolated from naturally fermented dairy products	213:281	A lactic acid bacteria strain AR333 with high-production of exopolysaccharides (EPS) was screened out from 350 bacteria strains isolated from naturally fermented dairy products.
26820354	1	27	attach	isolated	234:241	arg1	products					274:281	naturally fermented dairy products	248:281	naturally fermented dairy products	248:281	A lactic acid bacteria strain AR333 with high-production of exopolysaccharides (EPS) was screened out from 350 bacteria strains isolated from naturally fermented dairy products.
26820354	2	28	theme	Streptococcus	305:317	arg1	thermophilus					319:330	Streptococcus thermophilus	305:330	Streptococcus thermophilus	305:330	It was identified as Streptococcus thermophilus by morphological observation, physiological and biochemical characteristics, and 16S rDNA sequence determination.
26820354	2	28	theme	Streptococcus	305:317	arg1	It					284:285	It	284:285	It	284:285	It was identified as Streptococcus thermophilus by morphological observation, physiological and biochemical characteristics, and 16S rDNA sequence determination.
26820354	3	29	theme	Fast	502:505	arg1	Flow					507:510	DEAE-Sepharose Fast Flow	487:510	DEAE-Sepharose Fast Flow	487:510	The EPS from AR333 were purified through DEAE-Sepharose Fast Flow and Sepharose CL-6B, and the purified fraction was designated as EPS333.
26820354	5	30	theme	high-performance	842:857	arg1	chromatography					874:887	high-performance size exclusion chromatography	842:887	high-performance size exclusion chromatography (HPSEC)	842:895	The further work tried to elucidate the structural features of EPS333 via FT-IR spectrum, high-performance anion exchange chromatography (HPAEC) and high-performance size exclusion chromatography (HPSEC).
26820354	5	30	theme	high-performance	842:857	arg1	HPSEC					890:894	HPSEC	890:894	HPSEC	890:894	The further work tried to elucidate the structural features of EPS333 via FT-IR spectrum, high-performance anion exchange chromatography (HPAEC) and high-performance size exclusion chromatography (HPSEC).
26820354	6	31	theme	galactose	998:1006	arg1	composition					983:993	monosaccharide composition	968:993	monosaccharide composition of galactose and glucose	968:1018	The results showed that EPS333 was a pure neutral polysaccharide with monosaccharide composition of galactose and glucose in a molar ratio of 6:5.
26820354	1	32	theme	lactic	108:113	arg1	bacteria					120:127	lactic acid bacteria	108:127	A lactic acid bacteria strain AR333 with high-production of exopolysaccharides (EPS)	106:189	A lactic acid bacteria strain AR333 with high-production of exopolysaccharides (EPS) was screened out from 350 bacteria strains isolated from naturally fermented dairy products.
26820354	5	33	theme	exclusion	864:872	arg1	chromatography					874:887	high-performance size exclusion chromatography	842:887	high-performance size exclusion chromatography (HPSEC)	842:895	The further work tried to elucidate the structural features of EPS333 via FT-IR spectrum, high-performance anion exchange chromatography (HPAEC) and high-performance size exclusion chromatography (HPSEC).
26820354	5	33	theme	exclusion	864:872	arg1	HPSEC					890:894	HPSEC	890:894	HPSEC	890:894	The further work tried to elucidate the structural features of EPS333 via FT-IR spectrum, high-performance anion exchange chromatography (HPAEC) and high-performance size exclusion chromatography (HPSEC).
26820354	5	34	theme	anion	800:804	arg1	chromatography					815:828	high-performance anion exchange chromatography	783:828	high-performance anion exchange chromatography (HPAEC)	783:836	The further work tried to elucidate the structural features of EPS333 via FT-IR spectrum, high-performance anion exchange chromatography (HPAEC) and high-performance size exclusion chromatography (HPSEC).
26820354	5	34	theme	anion	800:804	arg1	HPAEC					831:835	HPAEC	831:835	HPAEC	831:835	The further work tried to elucidate the structural features of EPS333 via FT-IR spectrum, high-performance anion exchange chromatography (HPAEC) and high-performance size exclusion chromatography (HPSEC).
26820354	7	35	theme	groups	1072:1077	arg1	amount					1055:1060	A certain amount	1045:1060	A certain amount of acetyl groups	1045:1077	A certain amount of acetyl groups might exist in EPS333 according to the FT-IR spectrum.
26820354	7	35	theme	groups	1072:1077	arg1	groups					1072:1077	acetyl groups	1065:1077	acetyl groups	1065:1077	A certain amount of acetyl groups might exist in EPS333 according to the FT-IR spectrum.
26820354	9	36	theme	lactic	1222:1227	arg1	bacteria					1229:1236	lactic bacteria	1222:1236	the lactic bacteria strain AR333	1218:1249	Current study suggested that the lactic bacteria strain AR333 could be a potential source of immunoregulatory polysaccharide.
26820354	4	37	dep	significantly	669:681	arg1	p<0.05					684:689	p<0.05	684:689	p<0.05	684:689	In vitro test showed that EPS333 could stimulate macrophage RAW 264.7 to release NO significantly (p<0.05).
26820354	0	38	theme	S.	36:37	arg1	strain					52:57	a S. thermophilus strain	34:57	a S. thermophilus strain	34:57	Bioactive exopolysaccharides from a S. thermophilus strain: Screening, purification and characterization.
26820354	5	39	theme	exchange	806:813	arg1	chromatography					815:828	high-performance anion exchange chromatography	783:828	high-performance anion exchange chromatography (HPAEC)	783:836	The further work tried to elucidate the structural features of EPS333 via FT-IR spectrum, high-performance anion exchange chromatography (HPAEC) and high-performance size exclusion chromatography (HPSEC).
26820354	5	39	theme	exchange	806:813	arg1	HPAEC					831:835	HPAEC	831:835	HPAEC	831:835	The further work tried to elucidate the structural features of EPS333 via FT-IR spectrum, high-performance anion exchange chromatography (HPAEC) and high-performance size exclusion chromatography (HPSEC).
26820354	6	40	theme	molar	1025:1029	arg1	ratio					1031:1035	a molar ratio	1023:1035	a molar ratio of 6:5	1023:1042	The results showed that EPS333 was a pure neutral polysaccharide with monosaccharide composition of galactose and glucose in a molar ratio of 6:5.
26820354	3	41	theme	DEAE-Sepharose	487:500	arg1	Flow					507:510	DEAE-Sepharose Fast Flow	487:510	DEAE-Sepharose Fast Flow	487:510	The EPS from AR333 were purified through DEAE-Sepharose Fast Flow and Sepharose CL-6B, and the purified fraction was designated as EPS333.
26820354	6	42	theme	monosaccharide	968:981	arg1	composition					983:993	monosaccharide composition	968:993	monosaccharide composition of galactose and glucose	968:1018	The results showed that EPS333 was a pure neutral polysaccharide with monosaccharide composition of galactose and glucose in a molar ratio of 6:5.
26820354	9	43	theme	strain	1238:1243	arg1	source					1272:1277	a potential source	1260:1277	a potential source of immunoregulatory polysaccharide	1260:1312	Current study suggested that the lactic bacteria strain AR333 could be a potential source of immunoregulatory polysaccharide.
26820354	9	43	theme	strain	1238:1243	arg1	AR333					1245:1249	the lactic bacteria strain AR333	1218:1249	the lactic bacteria strain AR333	1218:1249	Current study suggested that the lactic bacteria strain AR333 could be a potential source of immunoregulatory polysaccharide.
26820354	2	44	theme	physiological	362:374	arg1	characteristics					392:406	physiological and biochemical characteristics	362:406	physiological and biochemical characteristics	362:406	It was identified as Streptococcus thermophilus by morphological observation, physiological and biochemical characteristics, and 16S rDNA sequence determination.
26820354	5	45	theme	structural	733:742	arg1	features					744:751	the structural features	729:751	the structural features of EPS333	729:761	The further work tried to elucidate the structural features of EPS333 via FT-IR spectrum, high-performance anion exchange chromatography (HPAEC) and high-performance size exclusion chromatography (HPSEC).
26820354	5	46	theme	further	697:703	arg1	work					705:708	The further work	693:708	The further work	693:708	The further work tried to elucidate the structural features of EPS333 via FT-IR spectrum, high-performance anion exchange chromatography (HPAEC) and high-performance size exclusion chromatography (HPSEC).
26820354	0	47	dep	S.	36:37	arg1	thermophilus					39:50	thermophilus	39:50	thermophilus	39:50	Bioactive exopolysaccharides from a S. thermophilus strain: Screening, purification and characterization.
26820354	1	48	theme	bacteria	120:127	arg1	AR333					136:140	A lactic acid bacteria strain AR333	106:140	A lactic acid bacteria strain AR333 with high-production of exopolysaccharides (EPS)	106:189	A lactic acid bacteria strain AR333 with high-production of exopolysaccharides (EPS) was screened out from 350 bacteria strains isolated from naturally fermented dairy products.
26820354	7	49	theme	certain	1047:1053	arg1	amount					1055:1060	A certain amount	1045:1060	A certain amount of acetyl groups	1045:1077	A certain amount of acetyl groups might exist in EPS333 according to the FT-IR spectrum.
26820354	7	49	theme	certain	1047:1053	arg1	groups					1072:1077	acetyl groups	1065:1077	acetyl groups	1065:1077	A certain amount of acetyl groups might exist in EPS333 according to the FT-IR spectrum.
26820354	1	50	theme	strain	129:134	arg1	AR333					136:140	A lactic acid bacteria strain AR333	106:140	A lactic acid bacteria strain AR333 with high-production of exopolysaccharides (EPS)	106:189	A lactic acid bacteria strain AR333 with high-production of exopolysaccharides (EPS) was screened out from 350 bacteria strains isolated from naturally fermented dairy products.
26820354	1	51	theme	bacteria	217:224	arg1	strains					226:232	350 bacteria strains	213:232	350 bacteria strains isolated from naturally fermented dairy products	213:281	A lactic acid bacteria strain AR333 with high-production of exopolysaccharides (EPS) was screened out from 350 bacteria strains isolated from naturally fermented dairy products.
26820354	2	52	theme	morphological	335:347	arg1	observation					349:359	morphological observation	335:359	morphological observation	335:359	It was identified as Streptococcus thermophilus by morphological observation, physiological and biochemical characteristics, and 16S rDNA sequence determination.
26820354	5	53	theme	FT-IR	767:771	arg1	spectrum					773:780	FT-IR spectrum	767:780	FT-IR spectrum	767:780	The further work tried to elucidate the structural features of EPS333 via FT-IR spectrum, high-performance anion exchange chromatography (HPAEC) and high-performance size exclusion chromatography (HPSEC).
26820354	6	54	from	polysaccharide	948:961	arg1	ratio					1031:1035	a molar ratio	1023:1035	a molar ratio of 6:5	1023:1042	The results showed that EPS333 was a pure neutral polysaccharide with monosaccharide composition of galactose and glucose in a molar ratio of 6:5.
26820354	0	55	dep	exopolysaccharides	10:27	arg1	purification					71:82	purification	71:82	purification	71:82	Bioactive exopolysaccharides from a S. thermophilus strain: Screening, purification and characterization.
26820354	0	55	dep	exopolysaccharides	10:27	arg1	Screening					60:68	Screening	60:68	Screening	60:68	Bioactive exopolysaccharides from a S. thermophilus strain: Screening, purification and characterization.
26820354	0	55	dep	exopolysaccharides	10:27	arg1	exopolysaccharides					10:27	Bioactive exopolysaccharides	0:27	Bioactive exopolysaccharides from a S. thermophilus strain: Screening, purification and characterization.	0:104	Bioactive exopolysaccharides from a S. thermophilus strain: Screening, purification and characterization.
26820354	0	55	dep	exopolysaccharides	10:27	arg1	characterization					88:103	characterization	88:103	characterization	88:103	Bioactive exopolysaccharides from a S. thermophilus strain: Screening, purification and characterization.
26820354	3	56	from	AR333	459:463	arg1	EPS					450:452	The EPS	446:452	The EPS from AR333	446:463	The EPS from AR333 were purified through DEAE-Sepharose Fast Flow and Sepharose CL-6B, and the purified fraction was designated as EPS333.
26820354	3	57	theme	purified	541:548	arg1	EPS333					577:582	EPS333	577:582	EPS333	577:582	The EPS from AR333 were purified through DEAE-Sepharose Fast Flow and Sepharose CL-6B, and the purified fraction was designated as EPS333.
26820354	3	57	theme	purified	541:548	arg1	fraction					550:557	the purified fraction	537:557	the purified fraction	537:557	The EPS from AR333 were purified through DEAE-Sepharose Fast Flow and Sepharose CL-6B, and the purified fraction was designated as EPS333.
26820354	6	58	theme	6:5	1040:1042	arg1	ratio					1031:1035	a molar ratio	1023:1035	a molar ratio of 6:5	1023:1042	The results showed that EPS333 was a pure neutral polysaccharide with monosaccharide composition of galactose and glucose in a molar ratio of 6:5.
26820354	8	59	theme	molecular	1138:1146	arg1	kDa					1184:1186	332 kDa	1180:1186	332 kDa	1180:1186	The molecular weight (Mw) was estimated to be 332 kDa.
26820354	8	59	theme	molecular	1138:1146	arg1	Mw					1156:1157	Mw	1156:1157	Mw	1156:1157	The molecular weight (Mw) was estimated to be 332 kDa.
26820354	8	59	theme	molecular	1138:1146	arg1	weight					1148:1153	The molecular weight	1134:1153	The molecular weight (Mw)	1134:1158	The molecular weight (Mw) was estimated to be 332 kDa.
26820354	6	60	theme	neutral	940:946	arg1	polysaccharide					948:961	a pure neutral polysaccharide	933:961	a pure neutral polysaccharide with monosaccharide composition of galactose and glucose	933:1018	The results showed that EPS333 was a pure neutral polysaccharide with monosaccharide composition of galactose and glucose in a molar ratio of 6:5.
26820354	6	60	theme	neutral	940:946	arg1	EPS333					922:927	EPS333	922:927	EPS333	922:927	The results showed that EPS333 was a pure neutral polysaccharide with monosaccharide composition of galactose and glucose in a molar ratio of 6:5.
26820354	4	61	theme	macrophage	634:643	arg1	RAW					645:647	macrophage RAW 264.7	634:653	macrophage RAW 264.7	634:653	In vitro test showed that EPS333 could stimulate macrophage RAW 264.7 to release NO significantly (p<0.05).
24510710	0	0	theme	polysaccharides	87:101	arg1	α-glucosidase					70:82	α-glucosidase	70:82	α-glucosidase of polysaccharides from fruit peel of tea (Camellia sinensis L.)	70:147	In vitro antioxidant activity and potential inhibitory action against α-glucosidase of polysaccharides from fruit peel of tea (Camellia sinensis L.).
24510710	4	1	from	activities	542:551	arg1	α-glucosidase					580:592	α-glucosidase	580:592	α-glucosidase of fractionated TFPPs (TFPP-0, TFPP-20, TFPP-40, and TFPP-60)	580:654	The physicochemical properties, in vitro antioxidant activities, and inhibitory effects on α-glucosidase of fractionated TFPPs (TFPP-0, TFPP-20, TFPP-40, and TFPP-60) were investigated.
24510710	6	2	theme	reducing	930:937	arg1	activity					939:946	reducing activity	930:946	reducing activity	930:946	They had not only remarkable scavenging activity on 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and reducing activity, but also excellent inhibitory potential against α-glucosidase in vitro.
24510710	1	3	theme	fruit	228:232	arg1	TFPPs					240:244	tea (Camellia sinensis L.) fruit peel (TFPPs)	201:245	tea (Camellia sinensis L.) fruit peel (TFPPs)	201:245	The conditions for extracting polysaccharides from tea (Camellia sinensis L.) fruit peel (TFPPs) were studied.
24510710	1	4	from	TFPPs	240:244	arg1	polysaccharides					180:194	polysaccharides	180:194	polysaccharides from tea (Camellia sinensis L.) fruit peel (TFPPs)	180:245	The conditions for extracting polysaccharides from tea (Camellia sinensis L.) fruit peel (TFPPs) were studied.
24510710	6	5	theme	2,2'-azinobis	849:861	arg1	ABTS					920:923	2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS)	849:924	2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS)	849:924	They had not only remarkable scavenging activity on 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and reducing activity, but also excellent inhibitory potential against α-glucosidase in vitro.
24510710	2	6	theme	liquid/solid	302:313	arg1	ratio					315:319	liquid/solid ratio	302:319	liquid/solid ratio	302:319	Three parameters (temperature, time, and liquid/solid ratio) affecting the extraction of TFPP were optimized using response surface methodology (RSM).
24510710	2	6	theme	liquid/solid	302:313	arg1	parameters					267:276	Three parameters	261:276	Three parameters (temperature, time, and liquid/solid ratio) affecting the extraction of TFPP	261:353	Three parameters (temperature, time, and liquid/solid ratio) affecting the extraction of TFPP were optimized using response surface methodology (RSM).
24510710	1	7	theme	peel	234:237	arg1	TFPPs					240:244	tea (Camellia sinensis L.) fruit peel (TFPPs)	201:245	tea (Camellia sinensis L.) fruit peel (TFPPs)	201:245	The conditions for extracting polysaccharides from tea (Camellia sinensis L.) fruit peel (TFPPs) were studied.
24510710	0	8	theme	fruit	108:112	arg1	peel					114:117	fruit peel	108:117	fruit peel of tea (Camellia sinensis L.)	108:147	In vitro antioxidant activity and potential inhibitory action against α-glucosidase of polysaccharides from fruit peel of tea (Camellia sinensis L.).
24510710	6	9	from	potential	979:987	arg1	ABTS					920:923	2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS)	849:924	2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS)	849:924	They had not only remarkable scavenging activity on 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and reducing activity, but also excellent inhibitory potential against α-glucosidase in vitro.
24510710	6	9	from	potential	979:987	arg1	activity					939:946	reducing activity	930:946	reducing activity	930:946	They had not only remarkable scavenging activity on 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and reducing activity, but also excellent inhibitory potential against α-glucosidase in vitro.
24510710	4	10	theme	fractionated	597:608	arg1	TFPPs					610:614	fractionated TFPPs	597:614	fractionated TFPPs (TFPP-0, TFPP-20, TFPP-40, and TFPP-60)	597:654	The physicochemical properties, in vitro antioxidant activities, and inhibitory effects on α-glucosidase of fractionated TFPPs (TFPP-0, TFPP-20, TFPP-40, and TFPP-60) were investigated.
24510710	4	10	theme	fractionated	597:608	arg1	TFPP-40					634:640	TFPP-40	634:640	TFPP-40	634:640	The physicochemical properties, in vitro antioxidant activities, and inhibitory effects on α-glucosidase of fractionated TFPPs (TFPP-0, TFPP-20, TFPP-40, and TFPP-60) were investigated.
24510710	4	10	theme	fractionated	597:608	arg1	TFPP-60					647:653	TFPP-60	647:653	TFPP-60	647:653	The physicochemical properties, in vitro antioxidant activities, and inhibitory effects on α-glucosidase of fractionated TFPPs (TFPP-0, TFPP-20, TFPP-40, and TFPP-60) were investigated.
24510710	4	11	theme	in	521:522	arg1	activities					542:551	in vitro antioxidant activities	521:551	in vitro antioxidant activities	521:551	The physicochemical properties, in vitro antioxidant activities, and inhibitory effects on α-glucosidase of fractionated TFPPs (TFPP-0, TFPP-20, TFPP-40, and TFPP-60) were investigated.
24510710	6	12	from	activity	837:844	arg1	ABTS					920:923	2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS)	849:924	2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS)	849:924	They had not only remarkable scavenging activity on 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and reducing activity, but also excellent inhibitory potential against α-glucosidase in vitro.
24510710	6	12	from	activity	837:844	arg1	activity					939:946	reducing activity	930:946	reducing activity	930:946	They had not only remarkable scavenging activity on 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and reducing activity, but also excellent inhibitory potential against α-glucosidase in vitro.
24510710	6	13	theme	scavenging	826:835	arg1	activity					837:844	remarkable scavenging activity	815:844	remarkable scavenging activity on 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and reducing activity	815:946	They had not only remarkable scavenging activity on 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and reducing activity, but also excellent inhibitory potential against α-glucosidase in vitro.
24510710	7	14	theme	fruit	1050:1054	arg1	bioresource					1093:1103	a potential bioresource	1081:1103	a potential bioresource for the development of polysaccharide antioxidants	1081:1154	Our results suggest that tea fruit peel could be treated as a potential bioresource for the development of polysaccharide antioxidants.
24510710	7	14	theme	fruit	1050:1054	arg1	peel					1056:1059	tea fruit peel	1046:1059	tea fruit peel	1046:1059	Our results suggest that tea fruit peel could be treated as a potential bioresource for the development of polysaccharide antioxidants.
24510710	5	15	theme	different	764:772	arg1	compositions					783:794	different chemical compositions	764:794	different chemical compositions	764:794	We found that the TFPPs were all acid protein-bound heteropolysaccharides, although with different chemical compositions.
24510710	2	16	theme	TFPP	350:353	arg1	extraction					336:345	the extraction	332:345	the extraction of TFPP	332:353	Three parameters (temperature, time, and liquid/solid ratio) affecting the extraction of TFPP were optimized using response surface methodology (RSM).
24510710	4	17	from	properties	509:518	arg1	α-glucosidase					580:592	α-glucosidase	580:592	α-glucosidase of fractionated TFPPs (TFPP-0, TFPP-20, TFPP-40, and TFPP-60)	580:654	The physicochemical properties, in vitro antioxidant activities, and inhibitory effects on α-glucosidase of fractionated TFPPs (TFPP-0, TFPP-20, TFPP-40, and TFPP-60) were investigated.
24510710	6	18	theme	remarkable	815:824	arg1	activity					837:844	remarkable scavenging activity	815:844	remarkable scavenging activity on 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and reducing activity	815:946	They had not only remarkable scavenging activity on 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and reducing activity, but also excellent inhibitory potential against α-glucosidase in vitro.
24510710	6	19	theme	inhibitory	968:977	arg1	potential					979:987	excellent inhibitory potential	958:987	excellent inhibitory potential	958:987	They had not only remarkable scavenging activity on 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and reducing activity, but also excellent inhibitory potential against α-glucosidase in vitro.
24510710	7	20	theme	antioxidants	1143:1154	arg1	development					1113:1123	the development	1109:1123	the development of polysaccharide antioxidants	1109:1154	Our results suggest that tea fruit peel could be treated as a potential bioresource for the development of polysaccharide antioxidants.
24510710	5	21	theme	protein-bound	713:725	arg1	heteropolysaccharides					727:747	all acid protein-bound heteropolysaccharides	704:747	all acid protein-bound heteropolysaccharides	704:747	We found that the TFPPs were all acid protein-bound heteropolysaccharides, although with different chemical compositions.
24510710	5	21	theme	protein-bound	713:725	arg1	TFPPs					693:697	the TFPPs	689:697	the TFPPs	689:697	We found that the TFPPs were all acid protein-bound heteropolysaccharides, although with different chemical compositions.
24510710	0	22	theme	In	0:1	arg1	activity					21:28	In vitro antioxidant activity	0:28	In vitro antioxidant activity	0:28	In vitro antioxidant activity and potential inhibitory action against α-glucosidase of polysaccharides from fruit peel of tea (Camellia sinensis L.).
24510710	7	23	theme	tea	1046:1048	arg1	bioresource					1093:1103	a potential bioresource	1081:1103	a potential bioresource for the development of polysaccharide antioxidants	1081:1154	Our results suggest that tea fruit peel could be treated as a potential bioresource for the development of polysaccharide antioxidants.
24510710	7	23	theme	tea	1046:1048	arg1	peel					1056:1059	tea fruit peel	1046:1059	tea fruit peel	1046:1059	Our results suggest that tea fruit peel could be treated as a potential bioresource for the development of polysaccharide antioxidants.
24510710	4	24	theme	TFPPs	610:614	arg1	α-glucosidase					580:592	α-glucosidase	580:592	α-glucosidase of fractionated TFPPs (TFPP-0, TFPP-20, TFPP-40, and TFPP-60)	580:654	The physicochemical properties, in vitro antioxidant activities, and inhibitory effects on α-glucosidase of fractionated TFPPs (TFPP-0, TFPP-20, TFPP-40, and TFPP-60) were investigated.
24510710	5	25	theme	chemical	774:781	arg1	compositions					783:794	different chemical compositions	764:794	different chemical compositions	764:794	We found that the TFPPs were all acid protein-bound heteropolysaccharides, although with different chemical compositions.
24510710	6	26	contain	had	802:804	arg2	potential					979:987	excellent inhibitory potential	958:987	excellent inhibitory potential	958:987	They had not only remarkable scavenging activity on 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and reducing activity, but also excellent inhibitory potential against α-glucosidase in vitro.
24510710	6	26	contain	had	802:804	arg1	They					797:800	They	797:800	They	797:800	They had not only remarkable scavenging activity on 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and reducing activity, but also excellent inhibitory potential against α-glucosidase in vitro.
24510710	6	26	contain	had	802:804	arg2	activity					837:844	remarkable scavenging activity	815:844	remarkable scavenging activity on 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and reducing activity	815:946	They had not only remarkable scavenging activity on 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and reducing activity, but also excellent inhibitory potential against α-glucosidase in vitro.
24510710	2	27	theme	response	376:383	arg1	RSM					406:408	RSM	406:408	RSM	406:408	Three parameters (temperature, time, and liquid/solid ratio) affecting the extraction of TFPP were optimized using response surface methodology (RSM).
24510710	2	27	theme	response	376:383	arg1	methodology					393:403	response surface methodology	376:403	response surface methodology (RSM)	376:409	Three parameters (temperature, time, and liquid/solid ratio) affecting the extraction of TFPP were optimized using response surface methodology (RSM).
24510710	2	28	theme	surface	385:391	arg1	RSM					406:408	RSM	406:408	RSM	406:408	Three parameters (temperature, time, and liquid/solid ratio) affecting the extraction of TFPP were optimized using response surface methodology (RSM).
24510710	2	28	theme	surface	385:391	arg1	methodology					393:403	response surface methodology	376:403	response surface methodology (RSM)	376:409	Three parameters (temperature, time, and liquid/solid ratio) affecting the extraction of TFPP were optimized using response surface methodology (RSM).
24510710	4	29	theme	physicochemical	493:507	arg1	properties					509:518	The physicochemical properties	489:518	The physicochemical properties	489:518	The physicochemical properties, in vitro antioxidant activities, and inhibitory effects on α-glucosidase of fractionated TFPPs (TFPP-0, TFPP-20, TFPP-40, and TFPP-60) were investigated.
24510710	0	30	theme	tea	122:124	arg1	peel					114:117	fruit peel	108:117	fruit peel of tea (Camellia sinensis L.)	108:147	In vitro antioxidant activity and potential inhibitory action against α-glucosidase of polysaccharides from fruit peel of tea (Camellia sinensis L.).
24510710	5	31	with	heteropolysaccharides	727:747	arg1	compositions					783:794	different chemical compositions	764:794	different chemical compositions	764:794	We found that the TFPPs were all acid protein-bound heteropolysaccharides, although with different chemical compositions.
24510710	4	32	from	effects	569:575	arg1	α-glucosidase					580:592	α-glucosidase	580:592	α-glucosidase of fractionated TFPPs (TFPP-0, TFPP-20, TFPP-40, and TFPP-60)	580:654	The physicochemical properties, in vitro antioxidant activities, and inhibitory effects on α-glucosidase of fractionated TFPPs (TFPP-0, TFPP-20, TFPP-40, and TFPP-60) were investigated.
24510710	4	33	theme	inhibitory	558:567	arg1	effects					569:575	inhibitory effects	558:575	inhibitory effects on α-glucosidase of fractionated TFPPs (TFPP-0, TFPP-20, TFPP-40, and TFPP-60)	558:654	The physicochemical properties, in vitro antioxidant activities, and inhibitory effects on α-glucosidase of fractionated TFPPs (TFPP-0, TFPP-20, TFPP-40, and TFPP-60) were investigated.
24510710	0	34	theme	potential	34:42	arg1	action					55:60	potential inhibitory action	34:60	potential inhibitory action	34:60	In vitro antioxidant activity and potential inhibitory action against α-glucosidase of polysaccharides from fruit peel of tea (Camellia sinensis L.).
24510710	0	35	theme	sinensis	136:143	arg1	tea					122:124	tea	122:124	tea (Camellia sinensis L.)	122:147	In vitro antioxidant activity and potential inhibitory action against α-glucosidase of polysaccharides from fruit peel of tea (Camellia sinensis L.).
24510710	0	35	theme	sinensis	136:143	arg1	L					145:145	Camellia sinensis L.	127:146	Camellia sinensis L.	127:146	In vitro antioxidant activity and potential inhibitory action against α-glucosidase of polysaccharides from fruit peel of tea (Camellia sinensis L.).
24510710	4	36	dep	in	521:522	arg1	vitro					524:528	vitro	524:528	vitro	524:528	The physicochemical properties, in vitro antioxidant activities, and inhibitory effects on α-glucosidase of fractionated TFPPs (TFPP-0, TFPP-20, TFPP-40, and TFPP-60) were investigated.
24510710	1	37	theme	tea	201:203	arg1	TFPPs					240:244	tea (Camellia sinensis L.) fruit peel (TFPPs)	201:245	tea (Camellia sinensis L.) fruit peel (TFPPs)	201:245	The conditions for extracting polysaccharides from tea (Camellia sinensis L.) fruit peel (TFPPs) were studied.
24510710	0	38	theme	antioxidant	9:19	arg1	activity					21:28	In vitro antioxidant activity	0:28	In vitro antioxidant activity	0:28	In vitro antioxidant activity and potential inhibitory action against α-glucosidase of polysaccharides from fruit peel of tea (Camellia sinensis L.).
24510710	6	39	theme	salt	914:917	arg1	ABTS					920:923	2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS)	849:924	2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS)	849:924	They had not only remarkable scavenging activity on 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and reducing activity, but also excellent inhibitory potential against α-glucosidase in vitro.
24510710	0	40	theme	Camellia	127:134	arg1	tea					122:124	tea	122:124	tea (Camellia sinensis L.)	122:147	In vitro antioxidant activity and potential inhibitory action against α-glucosidase of polysaccharides from fruit peel of tea (Camellia sinensis L.).
24510710	0	40	theme	Camellia	127:134	arg1	L					145:145	Camellia sinensis L.	127:146	Camellia sinensis L.	127:146	In vitro antioxidant activity and potential inhibitory action against α-glucosidase of polysaccharides from fruit peel of tea (Camellia sinensis L.).
24510710	0	41	from	α-glucosidase	70:82	arg1	peel					114:117	fruit peel	108:117	fruit peel of tea (Camellia sinensis L.)	108:147	In vitro antioxidant activity and potential inhibitory action against α-glucosidase of polysaccharides from fruit peel of tea (Camellia sinensis L.).
24510710	6	42	theme	diammonium	903:912	arg1	ABTS					920:923	2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS)	849:924	2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS)	849:924	They had not only remarkable scavenging activity on 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and reducing activity, but also excellent inhibitory potential against α-glucosidase in vitro.
24510710	5	43	theme	acid	708:711	arg1	heteropolysaccharides					727:747	all acid protein-bound heteropolysaccharides	704:747	all acid protein-bound heteropolysaccharides	704:747	We found that the TFPPs were all acid protein-bound heteropolysaccharides, although with different chemical compositions.
24510710	5	43	theme	acid	708:711	arg1	TFPPs					693:697	the TFPPs	689:697	the TFPPs	689:697	We found that the TFPPs were all acid protein-bound heteropolysaccharides, although with different chemical compositions.
24510710	2	44	dep	parameters	267:276	arg1	ratio					315:319	liquid/solid ratio	302:319	liquid/solid ratio	302:319	Three parameters (temperature, time, and liquid/solid ratio) affecting the extraction of TFPP were optimized using response surface methodology (RSM).
24510710	2	44	dep	parameters	267:276	arg1	parameters					267:276	Three parameters	261:276	Three parameters (temperature, time, and liquid/solid ratio) affecting the extraction of TFPP	261:353	Three parameters (temperature, time, and liquid/solid ratio) affecting the extraction of TFPP were optimized using response surface methodology (RSM).
24510710	2	44	dep	parameters	267:276	arg1	time					292:295	time	292:295	time	292:295	Three parameters (temperature, time, and liquid/solid ratio) affecting the extraction of TFPP were optimized using response surface methodology (RSM).
24510710	2	44	dep	parameters	267:276	arg1	temperature					279:289	temperature	279:289	temperature	279:289	Three parameters (temperature, time, and liquid/solid ratio) affecting the extraction of TFPP were optimized using response surface methodology (RSM).
24510710	0	45	theme	inhibitory	44:53	arg1	action					55:60	potential inhibitory action	34:60	potential inhibitory action	34:60	In vitro antioxidant activity and potential inhibitory action against α-glucosidase of polysaccharides from fruit peel of tea (Camellia sinensis L.).
24510710	1	46	dep	Camellia	206:213	arg1	L.					224:225	Camellia sinensis L.	206:225	Camellia sinensis L.	206:225	The conditions for extracting polysaccharides from tea (Camellia sinensis L.) fruit peel (TFPPs) were studied.
24510710	6	47	theme	acid	897:900	arg1	ABTS					920:923	2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS)	849:924	2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS)	849:924	They had not only remarkable scavenging activity on 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and reducing activity, but also excellent inhibitory potential against α-glucosidase in vitro.
24510710	0	48	from	peel	114:117	arg1	polysaccharides					87:101	polysaccharides	87:101	polysaccharides from fruit peel of tea (Camellia sinensis L.)	87:147	In vitro antioxidant activity and potential inhibitory action against α-glucosidase of polysaccharides from fruit peel of tea (Camellia sinensis L.).
24510710	0	48	from	peel	114:117	arg1	α-glucosidase					70:82	α-glucosidase	70:82	α-glucosidase of polysaccharides from fruit peel of tea (Camellia sinensis L.)	70:147	In vitro antioxidant activity and potential inhibitory action against α-glucosidase of polysaccharides from fruit peel of tea (Camellia sinensis L.).
24510710	7	49	theme	potential	1083:1091	arg1	bioresource					1093:1103	a potential bioresource	1081:1103	a potential bioresource for the development of polysaccharide antioxidants	1081:1154	Our results suggest that tea fruit peel could be treated as a potential bioresource for the development of polysaccharide antioxidants.
24510710	7	49	theme	potential	1083:1091	arg1	peel					1056:1059	tea fruit peel	1046:1059	tea fruit peel	1046:1059	Our results suggest that tea fruit peel could be treated as a potential bioresource for the development of polysaccharide antioxidants.
24510710	3	50	theme	optimized	422:430	arg1	conditions					432:441	the optimized conditions	418:441	the optimized conditions	418:441	Under the optimized conditions, the yield of TFPP was predicted to be 4.98%.
24510710	4	51	dep	TFPPs	610:614	arg1	TFPPs					610:614	fractionated TFPPs	597:614	fractionated TFPPs (TFPP-0, TFPP-20, TFPP-40, and TFPP-60)	597:654	The physicochemical properties, in vitro antioxidant activities, and inhibitory effects on α-glucosidase of fractionated TFPPs (TFPP-0, TFPP-20, TFPP-40, and TFPP-60) were investigated.
24510710	4	51	dep	TFPPs	610:614	arg1	TFPP-40					634:640	TFPP-40	634:640	TFPP-40	634:640	The physicochemical properties, in vitro antioxidant activities, and inhibitory effects on α-glucosidase of fractionated TFPPs (TFPP-0, TFPP-20, TFPP-40, and TFPP-60) were investigated.
24510710	4	51	dep	TFPPs	610:614	arg1	TFPP-60					647:653	TFPP-60	647:653	TFPP-60	647:653	The physicochemical properties, in vitro antioxidant activities, and inhibitory effects on α-glucosidase of fractionated TFPPs (TFPP-0, TFPP-20, TFPP-40, and TFPP-60) were investigated.
24510710	4	52	theme	antioxidant	530:540	arg1	activities					542:551	in vitro antioxidant activities	521:551	in vitro antioxidant activities	521:551	The physicochemical properties, in vitro antioxidant activities, and inhibitory effects on α-glucosidase of fractionated TFPPs (TFPP-0, TFPP-20, TFPP-40, and TFPP-60) were investigated.
24510710	3	53	theme	TFPP	457:460	arg1	%					486:486	4.98%	482:486	4.98%	482:486	Under the optimized conditions, the yield of TFPP was predicted to be 4.98%.
24510710	3	53	theme	TFPP	457:460	arg1	yield					448:452	the yield	444:452	the yield of TFPP	444:460	Under the optimized conditions, the yield of TFPP was predicted to be 4.98%.
24510710	6	54	theme	excellent	958:966	arg1	potential					979:987	excellent inhibitory potential	958:987	excellent inhibitory potential	958:987	They had not only remarkable scavenging activity on 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and reducing activity, but also excellent inhibitory potential against α-glucosidase in vitro.
24510710	6	55	theme	3-ethylbenzothiazoline-6-sulfonic	863:895	arg1	ABTS					920:923	2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS)	849:924	2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS)	849:924	They had not only remarkable scavenging activity on 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and reducing activity, but also excellent inhibitory potential against α-glucosidase in vitro.
24510710	0	56	dep	In	0:1	arg1	vitro					3:7	vitro	3:7	vitro	3:7	In vitro antioxidant activity and potential inhibitory action against α-glucosidase of polysaccharides from fruit peel of tea (Camellia sinensis L.).
24510710	7	57	theme	polysaccharide	1128:1141	arg1	antioxidants					1143:1154	polysaccharide antioxidants	1128:1154	polysaccharide antioxidants	1128:1154	Our results suggest that tea fruit peel could be treated as a potential bioresource for the development of polysaccharide antioxidants.
28498986	0	0	theme	biofilm	88:94	arg1	formation					96:104	biofilm formation	88:104	biofilm formation	88:104	Sequence of inoculation influences the nature of extracellular polymeric substances and biofilm formation in Azotobacter chroococcum and Trichoderma viride.
28498986	4	1	theme	i.e.	860:863	arg1	Az					865:866	Tv i.e. Az	857:866	Tv i.e. Az	857:866	The EPS produced during biofilm formation was found to differ quantitatively and qualitatively in individual cultures (Az alone, Tv alone), and in treatments differing in the sequence of inoculation of bacterium and fungus (Az + Tv coculture, staggered inoculation of Az followed by Tv i.e. Az - Tv, or Tv followed by Az i.e. Tv - Az).
28498986	2	2	theme	Trichoderma	378:388	arg1	Tv					398:399	Tv	398:399	Tv	398:399	In the present study, the EPS in biofilms developed using two agriculturally beneficial organisms-Azotobacter chroococcum (Az) and Trichoderma viride (Tv) were quantified and characterised.
28498986	2	2	theme	Trichoderma	378:388	arg1	viride					390:395	Trichoderma viride	378:395	Trichoderma viride (Tv)	378:400	In the present study, the EPS in biofilms developed using two agriculturally beneficial organisms-Azotobacter chroococcum (Az) and Trichoderma viride (Tv) were quantified and characterised.
28498986	4	3	theme	Tv	857:858	arg1	Az					865:866	Tv i.e. Az	857:866	Tv i.e. Az	857:866	The EPS produced during biofilm formation was found to differ quantitatively and qualitatively in individual cultures (Az alone, Tv alone), and in treatments differing in the sequence of inoculation of bacterium and fungus (Az + Tv coculture, staggered inoculation of Az followed by Tv i.e. Az - Tv, or Tv followed by Az i.e. Tv - Az).
28498986	6	4	theme	higher	1220:1225	arg1	carbohydrates					1233:1245	higher total carbohydrates	1220:1245	higher total carbohydrates	1220:1245	The EPS from biofilms showed significantly higher concentrations of protein, acetyl, and uronic acids, while planktonic EPS recorded higher total carbohydrates.
28498986	8	5	theme	cell	1462:1465	arg1	aggregation					1467:1477	cell aggregation	1462:1477	cell aggregation	1462:1477	This represents a first report correlating EPS production, cell aggregation and biofilm formation during bacterial-fungal biofilm development, which can have implications in the colonisation of soil and plants.
28498986	4	6	theme	bacterium	776:784	arg1	inoculation					761:771	inoculation	761:771	inoculation of bacterium and fungus	761:795	The EPS produced during biofilm formation was found to differ quantitatively and qualitatively in individual cultures (Az alone, Tv alone), and in treatments differing in the sequence of inoculation of bacterium and fungus (Az + Tv coculture, staggered inoculation of Az followed by Tv i.e. Az - Tv, or Tv followed by Az i.e. Tv - Az).
28498986	0	7	theme	formation	96:104	arg1	nature					39:44	the nature	35:44	the nature of extracellular polymeric substances and biofilm formation in Azotobacter chroococcum and Trichoderma viride	35:154	Sequence of inoculation influences the nature of extracellular polymeric substances and biofilm formation in Azotobacter chroococcum and Trichoderma viride.
28498986	8	8	theme	biofilm	1483:1489	arg1	formation					1491:1499	biofilm formation	1483:1499	biofilm formation	1483:1499	This represents a first report correlating EPS production, cell aggregation and biofilm formation during bacterial-fungal biofilm development, which can have implications in the colonisation of soil and plants.
28498986	6	9	theme	planktonic	1196:1205	arg1	EPS					1207:1209	planktonic EPS	1196:1209	planktonic EPS	1196:1209	The EPS from biofilms showed significantly higher concentrations of protein, acetyl, and uronic acids, while planktonic EPS recorded higher total carbohydrates.
28498986	8	10	theme	plants	1606:1611	arg1	colonisation					1581:1592	the colonisation	1577:1592	the colonisation of soil and plants	1577:1611	This represents a first report correlating EPS production, cell aggregation and biofilm formation during bacterial-fungal biofilm development, which can have implications in the colonisation of soil and plants.
28498986	5	11	theme	Significant	910:920	arg1	enhancement					922:932	Significant enhancement	910:932	Significant enhancement	910:932	Significant enhancement in terms of growth and biofilm formation, as compared to individual inoculation was recorded, with Tv - Az exhibiting higher values of these attributes.
28498986	6	12	from	biofilms	1100:1107	arg1	EPS					1091:1093	The EPS	1087:1093	The EPS from biofilms	1087:1107	The EPS from biofilms showed significantly higher concentrations of protein, acetyl, and uronic acids, while planktonic EPS recorded higher total carbohydrates.
28498986	7	13	theme	EPS	1373:1375	arg1	aspects					1362:1368	chemical and structural aspects	1338:1368	chemical and structural aspects of EPS (planktonic and biofilm)	1338:1400	Fourier transform infrared spectroscopy analyses illustrated the significant influence on chemical and structural aspects of EPS (planktonic and biofilm).
28498986	5	14	dep	enhancement	922:932	arg1	compared					979:986	compared	979:986	compared to individual inoculation	979:1012	Significant enhancement in terms of growth and biofilm formation, as compared to individual inoculation was recorded, with Tv - Az exhibiting higher values of these attributes.
28498986	4	15	theme	Az	842:843	arg1	inoculation					827:837	staggered inoculation	817:837	staggered inoculation of Az followed by Tv i.e. Az	817:866	The EPS produced during biofilm formation was found to differ quantitatively and qualitatively in individual cultures (Az alone, Tv alone), and in treatments differing in the sequence of inoculation of bacterium and fungus (Az + Tv coculture, staggered inoculation of Az followed by Tv i.e. Az - Tv, or Tv followed by Az i.e. Tv - Az).
28498986	3	16	theme	staggered	551:559	arg1	inoculation					561:571	staggered inoculation	551:571	staggered inoculation	551:571	Time course experiments were undertaken to optimise the EPS yield of biofilm samples resulting from coculture and staggered inoculation.
28498986	4	17	dep	Az	798:799	arg1	Az					905:906	Az	905:906	Az	905:906	The EPS produced during biofilm formation was found to differ quantitatively and qualitatively in individual cultures (Az alone, Tv alone), and in treatments differing in the sequence of inoculation of bacterium and fungus (Az + Tv coculture, staggered inoculation of Az followed by Tv i.e. Az - Tv, or Tv followed by Az i.e. Tv - Az).
28498986	4	17	dep	Az	798:799	arg1	Tv					870:871	Tv	870:871	Tv	870:871	The EPS produced during biofilm formation was found to differ quantitatively and qualitatively in individual cultures (Az alone, Tv alone), and in treatments differing in the sequence of inoculation of bacterium and fungus (Az + Tv coculture, staggered inoculation of Az followed by Tv i.e. Az - Tv, or Tv followed by Az i.e. Tv - Az).
28498986	4	17	dep	Az	798:799	arg1	Tv					877:878	Tv	877:878	Tv followed by Az i.e. Tv	877:901	The EPS produced during biofilm formation was found to differ quantitatively and qualitatively in individual cultures (Az alone, Tv alone), and in treatments differing in the sequence of inoculation of bacterium and fungus (Az + Tv coculture, staggered inoculation of Az followed by Tv i.e. Az - Tv, or Tv followed by Az i.e. Tv - Az).
28498986	4	17	dep	Az	798:799	arg1	inoculation					827:837	staggered inoculation	817:837	staggered inoculation of Az followed by Tv i.e. Az	817:866	The EPS produced during biofilm formation was found to differ quantitatively and qualitatively in individual cultures (Az alone, Tv alone), and in treatments differing in the sequence of inoculation of bacterium and fungus (Az + Tv coculture, staggered inoculation of Az followed by Tv i.e. Az - Tv, or Tv followed by Az i.e. Tv - Az).
28498986	7	18	theme	spectroscopy	1275:1286	arg1	analyses					1288:1295	spectroscopy analyses	1275:1295	spectroscopy analyses	1275:1295	Fourier transform infrared spectroscopy analyses illustrated the significant influence on chemical and structural aspects of EPS (planktonic and biofilm).
28498986	0	19	theme	Azotobacter	109:119	arg1	chroococcum					121:131	Azotobacter chroococcum	109:131	Azotobacter chroococcum	109:131	Sequence of inoculation influences the nature of extracellular polymeric substances and biofilm formation in Azotobacter chroococcum and Trichoderma viride.
28498986	0	20	from	nature	39:44	arg1	viride					149:154	Trichoderma viride	137:154	Trichoderma viride	137:154	Sequence of inoculation influences the nature of extracellular polymeric substances and biofilm formation in Azotobacter chroococcum and Trichoderma viride.
28498986	0	20	from	nature	39:44	arg1	chroococcum					121:131	Azotobacter chroococcum	109:131	Azotobacter chroococcum	109:131	Sequence of inoculation influences the nature of extracellular polymeric substances and biofilm formation in Azotobacter chroococcum and Trichoderma viride.
28498986	8	21	theme	bacterial-fungal	1508:1523	arg1	development					1533:1543	bacterial-fungal biofilm development	1508:1543	bacterial-fungal biofilm development	1508:1543	This represents a first report correlating EPS production, cell aggregation and biofilm formation during bacterial-fungal biofilm development, which can have implications in the colonisation of soil and plants.
28498986	6	22	theme	acids	1183:1187	arg1	concentrations					1137:1150	significantly higher concentrations	1116:1150	significantly higher concentrations of protein, acetyl, and uronic acids	1116:1187	The EPS from biofilms showed significantly higher concentrations of protein, acetyl, and uronic acids, while planktonic EPS recorded higher total carbohydrates.
28498986	2	23	theme	organisms-Azotobacter	335:355	arg1	Az					370:371	Az	370:371	Az	370:371	In the present study, the EPS in biofilms developed using two agriculturally beneficial organisms-Azotobacter chroococcum (Az) and Trichoderma viride (Tv) were quantified and characterised.
28498986	2	23	theme	organisms-Azotobacter	335:355	arg1	chroococcum					357:367	two agriculturally beneficial organisms-Azotobacter chroococcum	305:367	two agriculturally beneficial organisms-Azotobacter chroococcum (Az)	305:372	In the present study, the EPS in biofilms developed using two agriculturally beneficial organisms-Azotobacter chroococcum (Az) and Trichoderma viride (Tv) were quantified and characterised.
28498986	7	24	from	influence	1325:1333	arg1	aspects					1362:1368	chemical and structural aspects	1338:1368	chemical and structural aspects of EPS (planktonic and biofilm)	1338:1400	Fourier transform infrared spectroscopy analyses illustrated the significant influence on chemical and structural aspects of EPS (planktonic and biofilm).
28498986	3	25	theme	course	442:447	arg1	experiments					449:459	Time course experiments	437:459	Time course experiments	437:459	Time course experiments were undertaken to optimise the EPS yield of biofilm samples resulting from coculture and staggered inoculation.
28498986	8	26	theme	biofilm	1525:1531	arg1	development					1533:1543	bacterial-fungal biofilm development	1508:1543	bacterial-fungal biofilm development	1508:1543	This represents a first report correlating EPS production, cell aggregation and biofilm formation during bacterial-fungal biofilm development, which can have implications in the colonisation of soil and plants.
28498986	6	27	theme	uronic	1176:1181	arg1	acids					1183:1187	uronic acids	1176:1187	uronic acids	1176:1187	The EPS from biofilms showed significantly higher concentrations of protein, acetyl, and uronic acids, while planktonic EPS recorded higher total carbohydrates.
28498986	2	28	theme	beneficial	324:333	arg1	Az					370:371	Az	370:371	Az	370:371	In the present study, the EPS in biofilms developed using two agriculturally beneficial organisms-Azotobacter chroococcum (Az) and Trichoderma viride (Tv) were quantified and characterised.
28498986	2	28	theme	beneficial	324:333	arg1	chroococcum					357:367	two agriculturally beneficial organisms-Azotobacter chroococcum	305:367	two agriculturally beneficial organisms-Azotobacter chroococcum (Az)	305:372	In the present study, the EPS in biofilms developed using two agriculturally beneficial organisms-Azotobacter chroococcum (Az) and Trichoderma viride (Tv) were quantified and characterised.
28498986	1	29	theme	important	202:210	arg1	components					223:232	important structural components	202:232	important structural components of biofilms	202:244	Extracellular polymeric substances (EPS) are important structural components of biofilms.
28498986	1	29	theme	important	202:210	arg1	substances					181:190	Extracellular polymeric substances	157:190	Extracellular polymeric substances (EPS)	157:196	Extracellular polymeric substances (EPS) are important structural components of biofilms.
28498986	8	30	theme	EPS	1446:1448	arg1	production					1450:1459	EPS production	1446:1459	EPS production	1446:1459	This represents a first report correlating EPS production, cell aggregation and biofilm formation during bacterial-fungal biofilm development, which can have implications in the colonisation of soil and plants.
28498986	5	31	theme	growth	946:951	arg1	terms					937:941	terms	937:941	terms of growth and biofilm formation	937:973	Significant enhancement in terms of growth and biofilm formation, as compared to individual inoculation was recorded, with Tv - Az exhibiting higher values of these attributes.
28498986	0	32	theme	Trichoderma	137:147	arg1	viride					149:154	Trichoderma viride	137:154	Trichoderma viride	137:154	Sequence of inoculation influences the nature of extracellular polymeric substances and biofilm formation in Azotobacter chroococcum and Trichoderma viride.
28498986	7	33	theme	chemical	1338:1345	arg1	aspects					1362:1368	chemical and structural aspects	1338:1368	chemical and structural aspects of EPS (planktonic and biofilm)	1338:1400	Fourier transform infrared spectroscopy analyses illustrated the significant influence on chemical and structural aspects of EPS (planktonic and biofilm).
28498986	4	34	theme	inoculation	761:771	arg1	sequence					749:756	the sequence	745:756	the sequence of inoculation of bacterium and fungus	745:795	The EPS produced during biofilm formation was found to differ quantitatively and qualitatively in individual cultures (Az alone, Tv alone), and in treatments differing in the sequence of inoculation of bacterium and fungus (Az + Tv coculture, staggered inoculation of Az followed by Tv i.e. Az - Tv, or Tv followed by Az i.e. Tv - Az).
28498986	5	35	theme	biofilm	957:963	arg1	formation					965:973	biofilm formation	957:973	biofilm formation	957:973	Significant enhancement in terms of growth and biofilm formation, as compared to individual inoculation was recorded, with Tv - Az exhibiting higher values of these attributes.
28498986	1	36	theme	structural	212:221	arg1	components					223:232	important structural components	202:232	important structural components of biofilms	202:244	Extracellular polymeric substances (EPS) are important structural components of biofilms.
28498986	1	36	theme	structural	212:221	arg1	substances					181:190	Extracellular polymeric substances	157:190	Extracellular polymeric substances (EPS)	157:196	Extracellular polymeric substances (EPS) are important structural components of biofilms.
28498986	0	37	theme	inoculation	12:22	arg1	Sequence					0:7	Sequence	0:7	Sequence of inoculation	0:22	Sequence of inoculation influences the nature of extracellular polymeric substances and biofilm formation in Azotobacter chroococcum and Trichoderma viride.
28498986	4	38	theme	biofilm	598:604	arg1	formation					606:614	biofilm formation	598:614	biofilm formation	598:614	The EPS produced during biofilm formation was found to differ quantitatively and qualitatively in individual cultures (Az alone, Tv alone), and in treatments differing in the sequence of inoculation of bacterium and fungus (Az + Tv coculture, staggered inoculation of Az followed by Tv i.e. Az - Tv, or Tv followed by Az i.e. Tv - Az).
28498986	4	39	theme	fungus	790:795	arg1	inoculation					761:771	inoculation	761:771	inoculation of bacterium and fungus	761:795	The EPS produced during biofilm formation was found to differ quantitatively and qualitatively in individual cultures (Az alone, Tv alone), and in treatments differing in the sequence of inoculation of bacterium and fungus (Az + Tv coculture, staggered inoculation of Az followed by Tv i.e. Az - Tv, or Tv followed by Az i.e. Tv - Az).
28498986	2	40	from	EPS	273:275	arg1	biofilms					280:287	biofilms	280:287	biofilms developed using two agriculturally beneficial organisms-Azotobacter chroococcum (Az) and Trichoderma viride (Tv)	280:400	In the present study, the EPS in biofilms developed using two agriculturally beneficial organisms-Azotobacter chroococcum (Az) and Trichoderma viride (Tv) were quantified and characterised.
28498986	3	41	theme	EPS	493:495	arg1	yield					497:501	the EPS yield	489:501	the EPS yield of biofilm samples resulting from coculture and staggered inoculation	489:571	Time course experiments were undertaken to optimise the EPS yield of biofilm samples resulting from coculture and staggered inoculation.
28498986	4	42	theme	i.e.	895:898	arg1	Tv					900:901	Az i.e. Tv	892:901	Az i.e. Tv	892:901	The EPS produced during biofilm formation was found to differ quantitatively and qualitatively in individual cultures (Az alone, Tv alone), and in treatments differing in the sequence of inoculation of bacterium and fungus (Az + Tv coculture, staggered inoculation of Az followed by Tv i.e. Az - Tv, or Tv followed by Az i.e. Tv - Az).
28498986	2	43	theme	present	254:260	arg1	study					262:266	the present study	250:266	the present study	250:266	In the present study, the EPS in biofilms developed using two agriculturally beneficial organisms-Azotobacter chroococcum (Az) and Trichoderma viride (Tv) were quantified and characterised.
28498986	3	44	theme	Time	437:440	arg1	experiments					449:459	Time course experiments	437:459	Time course experiments	437:459	Time course experiments were undertaken to optimise the EPS yield of biofilm samples resulting from coculture and staggered inoculation.
28498986	5	45	theme	attributes	1075:1084	arg1	values					1059:1064	higher values	1052:1064	higher values of these attributes	1052:1084	Significant enhancement in terms of growth and biofilm formation, as compared to individual inoculation was recorded, with Tv - Az exhibiting higher values of these attributes.
28498986	4	46	theme	Az	892:893	arg1	Tv					900:901	Az i.e. Tv	892:901	Az i.e. Tv	892:901	The EPS produced during biofilm formation was found to differ quantitatively and qualitatively in individual cultures (Az alone, Tv alone), and in treatments differing in the sequence of inoculation of bacterium and fungus (Az + Tv coculture, staggered inoculation of Az followed by Tv i.e. Az - Tv, or Tv followed by Az i.e. Tv - Az).
28498986	4	47	dep	cultures	683:690	arg1	Az					693:694	Az	693:694	Az	693:694	The EPS produced during biofilm formation was found to differ quantitatively and qualitatively in individual cultures (Az alone, Tv alone), and in treatments differing in the sequence of inoculation of bacterium and fungus (Az + Tv coculture, staggered inoculation of Az followed by Tv i.e. Az - Tv, or Tv followed by Az i.e. Tv - Az).
28498986	6	48	dep	showed	1109:1114	arg1	while					1190:1194	while	1190:1194	while	1190:1194	The EPS from biofilms showed significantly higher concentrations of protein, acetyl, and uronic acids, while planktonic EPS recorded higher total carbohydrates.
28498986	1	49	theme	biofilms	237:244	arg1	components					223:232	important structural components	202:232	important structural components of biofilms	202:244	Extracellular polymeric substances (EPS) are important structural components of biofilms.
28498986	1	49	theme	biofilms	237:244	arg1	substances					181:190	Extracellular polymeric substances	157:190	Extracellular polymeric substances (EPS)	157:196	Extracellular polymeric substances (EPS) are important structural components of biofilms.
28498986	0	50	theme	extracellular	49:61	arg1	substances					73:82	extracellular polymeric substances	49:82	extracellular polymeric substances	49:82	Sequence of inoculation influences the nature of extracellular polymeric substances and biofilm formation in Azotobacter chroococcum and Trichoderma viride.
28498986	7	51	dep	EPS	1373:1375	arg1	biofilm					1393:1399	biofilm	1393:1399	biofilm	1393:1399	Fourier transform infrared spectroscopy analyses illustrated the significant influence on chemical and structural aspects of EPS (planktonic and biofilm).
28498986	7	51	dep	EPS	1373:1375	arg1	planktonic					1378:1387	planktonic	1378:1387	planktonic	1378:1387	Fourier transform infrared spectroscopy analyses illustrated the significant influence on chemical and structural aspects of EPS (planktonic and biofilm).
28498986	7	52	dep	Fourier	1248:1254	arg1	transform					1256:1264	transform	1256:1264	transform infrared spectroscopy analyses	1256:1295	Fourier transform infrared spectroscopy analyses illustrated the significant influence on chemical and structural aspects of EPS (planktonic and biofilm).
28498986	6	53	theme	acetyl	1164:1169	arg1	concentrations					1137:1150	significantly higher concentrations	1116:1150	significantly higher concentrations of protein, acetyl, and uronic acids	1116:1187	The EPS from biofilms showed significantly higher concentrations of protein, acetyl, and uronic acids, while planktonic EPS recorded higher total carbohydrates.
28498986	3	54	theme	biofilm	506:512	arg1	samples					514:520	biofilm samples	506:520	biofilm samples resulting from coculture and staggered inoculation	506:571	Time course experiments were undertaken to optimise the EPS yield of biofilm samples resulting from coculture and staggered inoculation.
28498986	4	55	theme	staggered	817:825	arg1	inoculation					827:837	staggered inoculation	817:837	staggered inoculation of Az followed by Tv i.e. Az	817:866	The EPS produced during biofilm formation was found to differ quantitatively and qualitatively in individual cultures (Az alone, Tv alone), and in treatments differing in the sequence of inoculation of bacterium and fungus (Az + Tv coculture, staggered inoculation of Az followed by Tv i.e. Az - Tv, or Tv followed by Az i.e. Tv - Az).
28498986	7	56	dep	transform	1256:1264	arg1	infrared					1266:1273	infrared	1266:1273	transform infrared spectroscopy analyses	1256:1295	Fourier transform infrared spectroscopy analyses illustrated the significant influence on chemical and structural aspects of EPS (planktonic and biofilm).
28498986	3	57	theme	samples	514:520	arg1	yield					497:501	the EPS yield	489:501	the EPS yield of biofilm samples resulting from coculture and staggered inoculation	489:571	Time course experiments were undertaken to optimise the EPS yield of biofilm samples resulting from coculture and staggered inoculation.
28498986	6	58	theme	higher	1130:1135	arg1	concentrations					1137:1150	significantly higher concentrations	1116:1150	significantly higher concentrations of protein, acetyl, and uronic acids	1116:1187	The EPS from biofilms showed significantly higher concentrations of protein, acetyl, and uronic acids, while planktonic EPS recorded higher total carbohydrates.
28498986	8	59	theme	soil	1597:1600	arg1	colonisation					1581:1592	the colonisation	1577:1592	the colonisation of soil and plants	1577:1611	This represents a first report correlating EPS production, cell aggregation and biofilm formation during bacterial-fungal biofilm development, which can have implications in the colonisation of soil and plants.
28498986	7	60	theme	significant	1313:1323	arg1	influence					1325:1333	the significant influence	1309:1333	the significant influence on chemical and structural aspects of EPS (planktonic and biofilm)	1309:1400	Fourier transform infrared spectroscopy analyses illustrated the significant influence on chemical and structural aspects of EPS (planktonic and biofilm).
28498986	6	61	theme	protein	1155:1161	arg1	concentrations					1137:1150	significantly higher concentrations	1116:1150	significantly higher concentrations of protein, acetyl, and uronic acids	1116:1187	The EPS from biofilms showed significantly higher concentrations of protein, acetyl, and uronic acids, while planktonic EPS recorded higher total carbohydrates.
28498986	5	62	theme	formation	965:973	arg1	terms					937:941	terms	937:941	terms of growth and biofilm formation	937:973	Significant enhancement in terms of growth and biofilm formation, as compared to individual inoculation was recorded, with Tv - Az exhibiting higher values of these attributes.
28498986	6	63	theme	total	1227:1231	arg1	carbohydrates					1233:1245	higher total carbohydrates	1220:1245	higher total carbohydrates	1220:1245	The EPS from biofilms showed significantly higher concentrations of protein, acetyl, and uronic acids, while planktonic EPS recorded higher total carbohydrates.
28498986	5	64	theme	individual	991:1000	arg1	inoculation					1002:1012	individual inoculation	991:1012	individual inoculation	991:1012	Significant enhancement in terms of growth and biofilm formation, as compared to individual inoculation was recorded, with Tv - Az exhibiting higher values of these attributes.
28498986	8	65	contain	have	1556:1559	arg1	development					1533:1543	bacterial-fungal biofilm development	1508:1543	bacterial-fungal biofilm development	1508:1543	This represents a first report correlating EPS production, cell aggregation and biofilm formation during bacterial-fungal biofilm development, which can have implications in the colonisation of soil and plants.
28498986	8	65	contain	have	1556:1559	arg2	implications					1561:1572	implications	1561:1572	implications	1561:1572	This represents a first report correlating EPS production, cell aggregation and biofilm formation during bacterial-fungal biofilm development, which can have implications in the colonisation of soil and plants.
28498986	8	66	theme	first	1421:1425	arg1	report					1427:1432	a first report	1419:1432	a first report correlating EPS production, cell aggregation and biofilm formation	1419:1499	This represents a first report correlating EPS production, cell aggregation and biofilm formation during bacterial-fungal biofilm development, which can have implications in the colonisation of soil and plants.
28498986	0	67	theme	substances	73:82	arg1	nature					39:44	the nature	35:44	the nature of extracellular polymeric substances and biofilm formation in Azotobacter chroococcum and Trichoderma viride	35:154	Sequence of inoculation influences the nature of extracellular polymeric substances and biofilm formation in Azotobacter chroococcum and Trichoderma viride.
28498986	4	68	dep	differ	629:634	arg1	Az					798:799	Az	798:799	Az	798:799	The EPS produced during biofilm formation was found to differ quantitatively and qualitatively in individual cultures (Az alone, Tv alone), and in treatments differing in the sequence of inoculation of bacterium and fungus (Az + Tv coculture, staggered inoculation of Az followed by Tv i.e. Az - Tv, or Tv followed by Az i.e. Tv - Az).
28498986	4	68	dep	differ	629:634	arg1	coculture					806:814	Tv coculture	803:814	Tv coculture	803:814	The EPS produced during biofilm formation was found to differ quantitatively and qualitatively in individual cultures (Az alone, Tv alone), and in treatments differing in the sequence of inoculation of bacterium and fungus (Az + Tv coculture, staggered inoculation of Az followed by Tv i.e. Az - Tv, or Tv followed by Az i.e. Tv - Az).
28498986	4	69	theme	Tv	803:804	arg1	coculture					806:814	Tv coculture	803:814	Tv coculture	803:814	The EPS produced during biofilm formation was found to differ quantitatively and qualitatively in individual cultures (Az alone, Tv alone), and in treatments differing in the sequence of inoculation of bacterium and fungus (Az + Tv coculture, staggered inoculation of Az followed by Tv i.e. Az - Tv, or Tv followed by Az i.e. Tv - Az).
28498986	4	70	theme	individual	672:681	arg1	cultures					683:690	individual cultures	672:690	individual cultures (Az alone, Tv alone)	672:711	The EPS produced during biofilm formation was found to differ quantitatively and qualitatively in individual cultures (Az alone, Tv alone), and in treatments differing in the sequence of inoculation of bacterium and fungus (Az + Tv coculture, staggered inoculation of Az followed by Tv i.e. Az - Tv, or Tv followed by Az i.e. Tv - Az).
28498986	1	71	theme	Extracellular	157:169	arg1	components					223:232	important structural components	202:232	important structural components of biofilms	202:244	Extracellular polymeric substances (EPS) are important structural components of biofilms.
28498986	1	71	theme	Extracellular	157:169	arg1	EPS					193:195	EPS	193:195	EPS	193:195	Extracellular polymeric substances (EPS) are important structural components of biofilms.
28498986	1	71	theme	Extracellular	157:169	arg1	substances					181:190	Extracellular polymeric substances	157:190	Extracellular polymeric substances (EPS)	157:196	Extracellular polymeric substances (EPS) are important structural components of biofilms.
28498986	0	72	theme	polymeric	63:71	arg1	substances					73:82	extracellular polymeric substances	49:82	extracellular polymeric substances	49:82	Sequence of inoculation influences the nature of extracellular polymeric substances and biofilm formation in Azotobacter chroococcum and Trichoderma viride.
28498986	5	73	theme	higher	1052:1057	arg1	values					1059:1064	higher values	1052:1064	higher values of these attributes	1052:1084	Significant enhancement in terms of growth and biofilm formation, as compared to individual inoculation was recorded, with Tv - Az exhibiting higher values of these attributes.
28498986	4	74	dep	Az	693:694	arg1	Tv					703:704	Tv	703:704	Tv alone	703:710	The EPS produced during biofilm formation was found to differ quantitatively and qualitatively in individual cultures (Az alone, Tv alone), and in treatments differing in the sequence of inoculation of bacterium and fungus (Az + Tv coculture, staggered inoculation of Az followed by Tv i.e. Az - Tv, or Tv followed by Az i.e. Tv - Az).
28498986	1	75	theme	polymeric	171:179	arg1	components					223:232	important structural components	202:232	important structural components of biofilms	202:244	Extracellular polymeric substances (EPS) are important structural components of biofilms.
28498986	1	75	theme	polymeric	171:179	arg1	EPS					193:195	EPS	193:195	EPS	193:195	Extracellular polymeric substances (EPS) are important structural components of biofilms.
28498986	1	75	theme	polymeric	171:179	arg1	substances					181:190	Extracellular polymeric substances	157:190	Extracellular polymeric substances (EPS)	157:196	Extracellular polymeric substances (EPS) are important structural components of biofilms.
28498986	5	76	from	enhancement	922:932	arg1	terms					937:941	terms	937:941	terms of growth and biofilm formation	937:973	Significant enhancement in terms of growth and biofilm formation, as compared to individual inoculation was recorded, with Tv - Az exhibiting higher values of these attributes.
28498986	7	77	theme	structural	1351:1360	arg1	aspects					1362:1368	chemical and structural aspects	1338:1368	chemical and structural aspects of EPS (planktonic and biofilm)	1338:1400	Fourier transform infrared spectroscopy analyses illustrated the significant influence on chemical and structural aspects of EPS (planktonic and biofilm).
26512808	3	0	theme	changes	339:345	arg1	influence					326:334	The influence	322:334	The influence of changes in cell properties and in the quality and composition of algal organic matter (AOM)	322:429	The influence of changes in cell properties and in the quality and composition of algal organic matter (AOM) were studied using Chlorella vulgaris as a model species.
26512808	4	1	theme	batch	492:496	arg1	cultivation					498:508	batch cultivation	492:508	batch cultivation	492:508	In batch cultivation, flocculation was increasingly inhibited over time and mainly influenced by changes in medium composition, rather than biological changes at the cell surface.
26512808	7	2	theme	complete	1116:1123	arg1	inhibition					1125:1134	a complete inhibition	1114:1134	a complete inhibition of flocculation	1114:1150	The addition of 30-50 mg L(-1) alginic acid, as a model for anionic carbohydrate polymers containing uronic acids, resulted in a complete inhibition of flocculation.
26512808	7	3	theme	30-50 mg L	1003:1012	arg1	acid					1026:1029	30-50 mg L(-1) alginic acid	1003:1029	30-50 mg L(-1) alginic acid	1003:1029	The addition of 30-50 mg L(-1) alginic acid, as a model for anionic carbohydrate polymers containing uronic acids, resulted in a complete inhibition of flocculation.
26512808	1	4	theme	microalgae	155:164	arg1	concentration					138:150	the concentration	134:150	the concentration of microalgae	134:164	Alkaline flocculation is a promising strategy for the concentration of microalgae for bulk biomass production.
26512808	7	5	theme	carbohydrate	1055:1066	arg1	polymers					1068:1075	anionic carbohydrate polymers	1047:1075	anionic carbohydrate polymers containing uronic acids	1047:1099	The addition of 30-50 mg L(-1) alginic acid, as a model for anionic carbohydrate polymers containing uronic acids, resulted in a complete inhibition of flocculation.
26512808	8	6	theme	anionic	1245:1251	arg1	polysaccharides					1253:1267	anionic polysaccharides	1245:1267	anionic polysaccharides leading to an increased flocculant demand over time	1245:1319	These results suggest that inhibition of alkaline flocculation was caused by interaction of anionic polysaccharides leading to an increased flocculant demand over time.
26512808	4	7	theme	medium	597:602	arg1	composition					604:614	medium composition	597:614	medium composition	597:614	In batch cultivation, flocculation was increasingly inhibited over time and mainly influenced by changes in medium composition, rather than biological changes at the cell surface.
26512808	6	8	theme	fraction	911:918	arg1	identification					888:901	The monosaccharide identification	869:901	The monosaccharide identification of this fraction	869:918	The monosaccharide identification of this fraction mainly showed the presence of neutral and anionic monosaccharides.
26512808	5	9	theme	Total	669:673	arg1	content					688:694	Total carbohydrate content	669:694	Total carbohydrate content of the organic matter fraction sized bigger than 3 kDa	669:749	Total carbohydrate content of the organic matter fraction sized bigger than 3 kDa increased over time and this fraction was shown to be mainly responsible for the inhibition of alkaline flocculation.
26512808	2	10	theme	flocculation	297:308	arg1	efficiency					310:319	flocculation efficiency	297:319	flocculation efficiency	297:319	However, previous studies have shown that biological changes during the cultivation negatively affect flocculation efficiency.
26512808	4	11	from	changes	586:592	arg1	composition					604:614	medium composition	597:614	medium composition	597:614	In batch cultivation, flocculation was increasingly inhibited over time and mainly influenced by changes in medium composition, rather than biological changes at the cell surface.
26512808	3	12	from	composition	389:399	arg1	influence					326:334	The influence	322:334	The influence of changes in cell properties and in the quality and composition of algal organic matter (AOM)	322:429	The influence of changes in cell properties and in the quality and composition of algal organic matter (AOM) were studied using Chlorella vulgaris as a model species.
26512808	1	13	theme	bulk	170:173	arg1	production					183:192	bulk biomass production	170:192	bulk biomass production	170:192	Alkaline flocculation is a promising strategy for the concentration of microalgae for bulk biomass production.
26512808	0	14	theme	organic	45:51	arg1	matter					53:58	algal organic matter	39:58	algal organic matter for Chlorella vulgaris	39:81	Inhibition of alkaline flocculation by algal organic matter for Chlorella vulgaris.
26512808	4	15	from	surface	660:666	arg1	changes					640:646	biological changes	629:646	biological changes at the cell surface	629:666	In batch cultivation, flocculation was increasingly inhibited over time and mainly influenced by changes in medium composition, rather than biological changes at the cell surface.
26512808	1	16	theme	biomass	175:181	arg1	production					183:192	bulk biomass production	170:192	bulk biomass production	170:192	Alkaline flocculation is a promising strategy for the concentration of microalgae for bulk biomass production.
26512808	8	17	theme	alkaline	1194:1201	arg1	flocculation					1203:1214	alkaline flocculation	1194:1214	alkaline flocculation	1194:1214	These results suggest that inhibition of alkaline flocculation was caused by interaction of anionic polysaccharides leading to an increased flocculant demand over time.
26512808	7	18	theme	uronic	1088:1093	arg1	acids					1095:1099	uronic acids	1088:1099	uronic acids	1088:1099	The addition of 30-50 mg L(-1) alginic acid, as a model for anionic carbohydrate polymers containing uronic acids, resulted in a complete inhibition of flocculation.
26512808	0	19	theme	Chlorella	64:72	arg1	vulgaris					74:81	Chlorella vulgaris	64:81	Chlorella vulgaris	64:81	Inhibition of alkaline flocculation by algal organic matter for Chlorella vulgaris.
26512808	1	20	theme	Alkaline	84:91	arg1	flocculation					93:104	Alkaline flocculation	84:104	Alkaline flocculation	84:104	Alkaline flocculation is a promising strategy for the concentration of microalgae for bulk biomass production.
26512808	1	20	theme	Alkaline	84:91	arg1	strategy					121:128	a promising strategy	109:128	a promising strategy for the concentration of microalgae for bulk biomass production	109:192	Alkaline flocculation is a promising strategy for the concentration of microalgae for bulk biomass production.
26512808	3	21	theme	algal	404:408	arg1	matter					418:423	algal organic matter	404:423	algal organic matter (AOM)	404:429	The influence of changes in cell properties and in the quality and composition of algal organic matter (AOM) were studied using Chlorella vulgaris as a model species.
26512808	3	21	theme	algal	404:408	arg1	AOM					426:428	AOM	426:428	AOM	426:428	The influence of changes in cell properties and in the quality and composition of algal organic matter (AOM) were studied using Chlorella vulgaris as a model species.
26512808	8	22	theme	polysaccharides	1253:1267	arg1	interaction					1230:1240	interaction	1230:1240	interaction of anionic polysaccharides leading to an increased flocculant demand over time	1230:1319	These results suggest that inhibition of alkaline flocculation was caused by interaction of anionic polysaccharides leading to an increased flocculant demand over time.
26512808	0	23	theme	flocculation	23:34	arg1	Inhibition					0:9	Inhibition	0:9	Inhibition of alkaline flocculation by algal organic matter for Chlorella vulgaris.	0:82	Inhibition of alkaline flocculation by algal organic matter for Chlorella vulgaris.
26512808	5	24	theme	organic	703:709	arg1	fraction					718:725	the organic matter fraction	699:725	the organic matter fraction sized bigger than 3 kDa	699:749	Total carbohydrate content of the organic matter fraction sized bigger than 3 kDa increased over time and this fraction was shown to be mainly responsible for the inhibition of alkaline flocculation.
26512808	8	25	theme	increased	1283:1291	arg1	demand					1304:1309	an increased flocculant demand	1280:1309	an increased flocculant demand over time	1280:1319	These results suggest that inhibition of alkaline flocculation was caused by interaction of anionic polysaccharides leading to an increased flocculant demand over time.
26512808	0	26	theme	alkaline	14:21	arg1	flocculation					23:34	alkaline flocculation	14:34	alkaline flocculation	14:34	Inhibition of alkaline flocculation by algal organic matter for Chlorella vulgaris.
26512808	7	27	theme	anionic	1047:1053	arg1	polymers					1068:1075	anionic carbohydrate polymers	1047:1075	anionic carbohydrate polymers containing uronic acids	1047:1099	The addition of 30-50 mg L(-1) alginic acid, as a model for anionic carbohydrate polymers containing uronic acids, resulted in a complete inhibition of flocculation.
26512808	5	28	theme	matter	711:716	arg1	fraction					718:725	the organic matter fraction	699:725	the organic matter fraction sized bigger than 3 kDa	699:749	Total carbohydrate content of the organic matter fraction sized bigger than 3 kDa increased over time and this fraction was shown to be mainly responsible for the inhibition of alkaline flocculation.
26512808	6	29	theme	monosaccharide	873:886	arg1	identification					888:901	The monosaccharide identification	869:901	The monosaccharide identification of this fraction	869:918	The monosaccharide identification of this fraction mainly showed the presence of neutral and anionic monosaccharides.
26512808	7	30	contain	containing	1077:1086	arg1	polymers					1068:1075	anionic carbohydrate polymers	1047:1075	anionic carbohydrate polymers containing uronic acids	1047:1099	The addition of 30-50 mg L(-1) alginic acid, as a model for anionic carbohydrate polymers containing uronic acids, resulted in a complete inhibition of flocculation.
26512808	7	30	contain	containing	1077:1086	arg2	acids					1095:1099	uronic acids	1088:1099	uronic acids	1088:1099	The addition of 30-50 mg L(-1) alginic acid, as a model for anionic carbohydrate polymers containing uronic acids, resulted in a complete inhibition of flocculation.
26512808	4	31	theme	biological	629:638	arg1	changes					640:646	biological changes	629:646	biological changes at the cell surface	629:666	In batch cultivation, flocculation was increasingly inhibited over time and mainly influenced by changes in medium composition, rather than biological changes at the cell surface.
26512808	0	32	theme	algal	39:43	arg1	matter					53:58	algal organic matter	39:58	algal organic matter for Chlorella vulgaris	39:81	Inhibition of alkaline flocculation by algal organic matter for Chlorella vulgaris.
26512808	6	33	theme	monosaccharides	970:984	arg1	presence					938:945	the presence	934:945	the presence of neutral and anionic monosaccharides	934:984	The monosaccharide identification of this fraction mainly showed the presence of neutral and anionic monosaccharides.
26512808	5	34	theme	fraction	718:725	arg1	content					688:694	Total carbohydrate content	669:694	Total carbohydrate content of the organic matter fraction sized bigger than 3 kDa	669:749	Total carbohydrate content of the organic matter fraction sized bigger than 3 kDa increased over time and this fraction was shown to be mainly responsible for the inhibition of alkaline flocculation.
26512808	2	35	theme	biological	237:246	arg1	changes					248:254	biological changes	237:254	biological changes during the cultivation	237:277	However, previous studies have shown that biological changes during the cultivation negatively affect flocculation efficiency.
26512808	5	36	theme	flocculation	855:866	arg1	inhibition					832:841	the inhibition	828:841	the inhibition of alkaline flocculation	828:866	Total carbohydrate content of the organic matter fraction sized bigger than 3 kDa increased over time and this fraction was shown to be mainly responsible for the inhibition of alkaline flocculation.
26512808	6	37	theme	anionic	962:968	arg1	monosaccharides					970:984	neutral and anionic monosaccharides	950:984	neutral and anionic monosaccharides	950:984	The monosaccharide identification of this fraction mainly showed the presence of neutral and anionic monosaccharides.
26512808	5	38	theme	sized	727:731	arg1	fraction					718:725	the organic matter fraction	699:725	the organic matter fraction sized bigger than 3 kDa	699:749	Total carbohydrate content of the organic matter fraction sized bigger than 3 kDa increased over time and this fraction was shown to be mainly responsible for the inhibition of alkaline flocculation.
26512808	7	39	theme	acid	1026:1029	arg1	addition					991:998	The addition	987:998	The addition of 30-50 mg L(-1) alginic acid	987:1029	The addition of 30-50 mg L(-1) alginic acid, as a model for anionic carbohydrate polymers containing uronic acids, resulted in a complete inhibition of flocculation.
26512808	5	40	theme	alkaline	846:853	arg1	flocculation					855:866	alkaline flocculation	846:866	alkaline flocculation	846:866	Total carbohydrate content of the organic matter fraction sized bigger than 3 kDa increased over time and this fraction was shown to be mainly responsible for the inhibition of alkaline flocculation.
26512808	3	41	theme	organic	410:416	arg1	matter					418:423	algal organic matter	404:423	algal organic matter (AOM)	404:429	The influence of changes in cell properties and in the quality and composition of algal organic matter (AOM) were studied using Chlorella vulgaris as a model species.
26512808	3	41	theme	organic	410:416	arg1	AOM					426:428	AOM	426:428	AOM	426:428	The influence of changes in cell properties and in the quality and composition of algal organic matter (AOM) were studied using Chlorella vulgaris as a model species.
26512808	5	42	theme	carbohydrate	675:686	arg1	content					688:694	Total carbohydrate content	669:694	Total carbohydrate content of the organic matter fraction sized bigger than 3 kDa	669:749	Total carbohydrate content of the organic matter fraction sized bigger than 3 kDa increased over time and this fraction was shown to be mainly responsible for the inhibition of alkaline flocculation.
26512808	3	43	from	quality	377:383	arg1	influence					326:334	The influence	322:334	The influence of changes in cell properties and in the quality and composition of algal organic matter (AOM)	322:429	The influence of changes in cell properties and in the quality and composition of algal organic matter (AOM) were studied using Chlorella vulgaris as a model species.
26512808	1	44	theme	promising	111:119	arg1	flocculation					93:104	Alkaline flocculation	84:104	Alkaline flocculation	84:104	Alkaline flocculation is a promising strategy for the concentration of microalgae for bulk biomass production.
26512808	1	44	theme	promising	111:119	arg1	strategy					121:128	a promising strategy	109:128	a promising strategy for the concentration of microalgae for bulk biomass production	109:192	Alkaline flocculation is a promising strategy for the concentration of microalgae for bulk biomass production.
26512808	3	45	theme	matter	418:423	arg1	composition					389:399	composition	389:399	composition	389:399	The influence of changes in cell properties and in the quality and composition of algal organic matter (AOM) were studied using Chlorella vulgaris as a model species.
26512808	3	45	theme	matter	418:423	arg1	quality					377:383	quality	377:383	quality	377:383	The influence of changes in cell properties and in the quality and composition of algal organic matter (AOM) were studied using Chlorella vulgaris as a model species.
26512808	6	46	theme	neutral	950:956	arg1	monosaccharides					970:984	neutral and anionic monosaccharides	950:984	neutral and anionic monosaccharides	950:984	The monosaccharide identification of this fraction mainly showed the presence of neutral and anionic monosaccharides.
26512808	3	47	from	changes	339:345	arg1	properties					355:364	cell properties	350:364	cell properties	350:364	The influence of changes in cell properties and in the quality and composition of algal organic matter (AOM) were studied using Chlorella vulgaris as a model species.
26512808	3	47	from	changes	339:345	arg1	composition					389:399	composition	389:399	composition	389:399	The influence of changes in cell properties and in the quality and composition of algal organic matter (AOM) were studied using Chlorella vulgaris as a model species.
26512808	3	47	from	changes	339:345	arg1	quality					377:383	quality	377:383	quality	377:383	The influence of changes in cell properties and in the quality and composition of algal organic matter (AOM) were studied using Chlorella vulgaris as a model species.
26512808	2	48	theme	previous	204:211	arg1	studies					213:219	previous studies	204:219	previous studies	204:219	However, previous studies have shown that biological changes during the cultivation negatively affect flocculation efficiency.
26512808	7	49	theme	flocculation	1139:1150	arg1	inhibition					1125:1134	a complete inhibition	1114:1134	a complete inhibition of flocculation	1114:1150	The addition of 30-50 mg L(-1) alginic acid, as a model for anionic carbohydrate polymers containing uronic acids, resulted in a complete inhibition of flocculation.
26512808	7	50	theme	alginic	1018:1024	arg1	acid					1026:1029	30-50 mg L(-1) alginic acid	1003:1029	30-50 mg L(-1) alginic acid	1003:1029	The addition of 30-50 mg L(-1) alginic acid, as a model for anionic carbohydrate polymers containing uronic acids, resulted in a complete inhibition of flocculation.
26512808	3	51	theme	model	474:478	arg1	vulgaris					460:467	Chlorella vulgaris	450:467	Chlorella vulgaris	450:467	The influence of changes in cell properties and in the quality and composition of algal organic matter (AOM) were studied using Chlorella vulgaris as a model species.
26512808	3	51	theme	model	474:478	arg1	species					480:486	a model species	472:486	a model species	472:486	The influence of changes in cell properties and in the quality and composition of algal organic matter (AOM) were studied using Chlorella vulgaris as a model species.
26512808	8	52	theme	flocculant	1293:1302	arg1	demand					1304:1309	an increased flocculant demand	1280:1309	an increased flocculant demand over time	1280:1319	These results suggest that inhibition of alkaline flocculation was caused by interaction of anionic polysaccharides leading to an increased flocculant demand over time.
26512808	3	53	dep	quality	377:383	arg1	the					373:375	the	373:375	the	373:375	The influence of changes in cell properties and in the quality and composition of algal organic matter (AOM) were studied using Chlorella vulgaris as a model species.
26512808	3	54	theme	cell	350:353	arg1	properties					355:364	cell properties	350:364	cell properties	350:364	The influence of changes in cell properties and in the quality and composition of algal organic matter (AOM) were studied using Chlorella vulgaris as a model species.
26512808	3	55	from	properties	355:364	arg1	influence					326:334	The influence	322:334	The influence of changes in cell properties and in the quality and composition of algal organic matter (AOM)	322:429	The influence of changes in cell properties and in the quality and composition of algal organic matter (AOM) were studied using Chlorella vulgaris as a model species.
26512808	4	56	theme	cell	655:658	arg1	surface					660:666	the cell surface	651:666	the cell surface	651:666	In batch cultivation, flocculation was increasingly inhibited over time and mainly influenced by changes in medium composition, rather than biological changes at the cell surface.
26512808	3	57	from	influence	326:334	arg1	properties					355:364	cell properties	350:364	cell properties	350:364	The influence of changes in cell properties and in the quality and composition of algal organic matter (AOM) were studied using Chlorella vulgaris as a model species.
26512808	3	57	from	influence	326:334	arg1	composition					389:399	composition	389:399	composition	389:399	The influence of changes in cell properties and in the quality and composition of algal organic matter (AOM) were studied using Chlorella vulgaris as a model species.
26512808	3	57	from	influence	326:334	arg1	quality					377:383	quality	377:383	quality	377:383	The influence of changes in cell properties and in the quality and composition of algal organic matter (AOM) were studied using Chlorella vulgaris as a model species.
26512808	5	58	dep	sized	727:731	arg1	bigger					733:738	bigger	733:738	bigger	733:738	Total carbohydrate content of the organic matter fraction sized bigger than 3 kDa increased over time and this fraction was shown to be mainly responsible for the inhibition of alkaline flocculation.
26512808	8	59	theme	flocculation	1203:1214	arg1	inhibition					1180:1189	inhibition	1180:1189	inhibition of alkaline flocculation	1180:1214	These results suggest that inhibition of alkaline flocculation was caused by interaction of anionic polysaccharides leading to an increased flocculant demand over time.
26572460	0	0	theme	n-methyl-2-pyrrolidone/water	92:119	arg1	mixtures					121:128	n-methyl-2-pyrrolidone/water mixtures	92:128	n-methyl-2-pyrrolidone/water mixtures	92:128	Solvent resistant chitosan/poly(ether-block-amide) composite membranes for pervaporation of n-methyl-2-pyrrolidone/water mixtures.
26572460	4	1	theme	method	725:730	arg1	model					738:742	A two-dimensional finite element method (FEM) model	692:742	A two-dimensional finite element method (FEM) model	692:742	A two-dimensional finite element method (FEM) model was developed to predict the concentration profile within the membrane through computational fluid dynamics (CFD).
26572460	4	2	theme	FEM	733:735	arg1	model					738:742	A two-dimensional finite element method (FEM) model	692:742	A two-dimensional finite element method (FEM) model	692:742	A two-dimensional finite element method (FEM) model was developed to predict the concentration profile within the membrane through computational fluid dynamics (CFD).
26572460	6	3	dep	chitosan	1088:1095	arg1	membranes					1148:1156	crosslinked composite membranes	1126:1156	crosslinked composite membranes	1126:1156	Both unmodified chitosan and tetraethyl orthosilicate crosslinked composite membranes successfully separated feed mixture containing 4.6 wt% water by exhibiting water fluxes of 0.024 and 0.019 kg/m(2)h, whereas the corresponding selectivities were found to be as high as 182 and 225, respectively.
26572460	6	4	link	crosslinked	1126:1136	arg1	membranes					1148:1156	crosslinked composite membranes	1126:1156	crosslinked composite membranes	1126:1156	Both unmodified chitosan and tetraethyl orthosilicate crosslinked composite membranes successfully separated feed mixture containing 4.6 wt% water by exhibiting water fluxes of 0.024 and 0.019 kg/m(2)h, whereas the corresponding selectivities were found to be as high as 182 and 225, respectively.
26572460	1	5	theme	resistant	195:203	arg1	CS					215:216	CS	215:216	CS	215:216	A novel composite barrier comprising of hydrophilic and solvent resistant chitosan (CS) membrane on porous solvent resistant poly(ether-block-amide) (PEBA-2533) substrate was synthesized for pervaporation (PV) based dehydration of the polar aprotic n-methyl-2-pyrolidone (NMP) green solvent.
26572460	1	5	theme	resistant	195:203	arg1	chitosan					205:212	hydrophilic and solvent resistant chitosan	171:212	hydrophilic and solvent resistant chitosan (CS) membrane on porous solvent resistant poly(ether-block-amide) (PEBA-2533) substrate	171:300	A novel composite barrier comprising of hydrophilic and solvent resistant chitosan (CS) membrane on porous solvent resistant poly(ether-block-amide) (PEBA-2533) substrate was synthesized for pervaporation (PV) based dehydration of the polar aprotic n-methyl-2-pyrolidone (NMP) green solvent.
26572460	6	6	dep	separated	1171:1179	arg1	whereas					1275:1281	whereas	1275:1281	whereas	1275:1281	Both unmodified chitosan and tetraethyl orthosilicate crosslinked composite membranes successfully separated feed mixture containing 4.6 wt% water by exhibiting water fluxes of 0.024 and 0.019 kg/m(2)h, whereas the corresponding selectivities were found to be as high as 182 and 225, respectively.
26572460	6	7	theme	2	1270:1270	arg1	h					1272:1272	0.024 and 0.019 kg/m(2)h	1249:1272	0.024 and 0.019 kg/m(2)h	1249:1272	Both unmodified chitosan and tetraethyl orthosilicate crosslinked composite membranes successfully separated feed mixture containing 4.6 wt% water by exhibiting water fluxes of 0.024 and 0.019 kg/m(2)h, whereas the corresponding selectivities were found to be as high as 182 and 225, respectively.
26572460	5	8	theme	numerical	906:914	arg1	simulation					916:925	a numerical simulation	904:925	a numerical simulation	904:925	On the basis of complete mixing experiments, a numerical simulation was performed to predict membrane area requirement and exit streams' compositions for commercial pervaporation units operated in plug flow mode.
26572460	6	9	theme	wt	1209:1210	arg1	%					1211:1211	4.6 wt%	1205:1211	4.6 wt% water	1205:1217	Both unmodified chitosan and tetraethyl orthosilicate crosslinked composite membranes successfully separated feed mixture containing 4.6 wt% water by exhibiting water fluxes of 0.024 and 0.019 kg/m(2)h, whereas the corresponding selectivities were found to be as high as 182 and 225, respectively.
26572460	6	10	theme	h	1272:1272	arg1	fluxes					1239:1244	water fluxes	1233:1244	water fluxes of 0.024 and 0.019 kg/m(2)h	1233:1272	Both unmodified chitosan and tetraethyl orthosilicate crosslinked composite membranes successfully separated feed mixture containing 4.6 wt% water by exhibiting water fluxes of 0.024 and 0.019 kg/m(2)h, whereas the corresponding selectivities were found to be as high as 182 and 225, respectively.
26572460	0	11	theme	mixtures	121:128	arg1	pervaporation					75:87	pervaporation	75:87	pervaporation of n-methyl-2-pyrrolidone/water mixtures	75:128	Solvent resistant chitosan/poly(ether-block-amide) composite membranes for pervaporation of n-methyl-2-pyrrolidone/water mixtures.
26572460	1	12	theme	polar	366:370	arg1	solvent					414:420	the polar aprotic n-methyl-2-pyrolidone (NMP) green solvent	362:420	the polar aprotic n-methyl-2-pyrolidone (NMP) green solvent	362:420	A novel composite barrier comprising of hydrophilic and solvent resistant chitosan (CS) membrane on porous solvent resistant poly(ether-block-amide) (PEBA-2533) substrate was synthesized for pervaporation (PV) based dehydration of the polar aprotic n-methyl-2-pyrolidone (NMP) green solvent.
26572460	3	13	theme	feed	572:575	arg1	concentration					583:595	feed water concentration	572:595	feed water concentration	572:595	Operating parameters such as feed water concentration, permeate pressure and membrane thickness were varied to assess membrane flux and selectivity.
26572460	5	14	theme	membrane	952:959	arg1	requirement					966:976	membrane area requirement	952:976	membrane area requirement	952:976	On the basis of complete mixing experiments, a numerical simulation was performed to predict membrane area requirement and exit streams' compositions for commercial pervaporation units operated in plug flow mode.
26572460	1	15	theme	aprotic	372:378	arg1	solvent					414:420	the polar aprotic n-methyl-2-pyrolidone (NMP) green solvent	362:420	the polar aprotic n-methyl-2-pyrolidone (NMP) green solvent	362:420	A novel composite barrier comprising of hydrophilic and solvent resistant chitosan (CS) membrane on porous solvent resistant poly(ether-block-amide) (PEBA-2533) substrate was synthesized for pervaporation (PV) based dehydration of the polar aprotic n-methyl-2-pyrolidone (NMP) green solvent.
26572460	6	16	theme	corresponding	1287:1299	arg1	high					1335:1338	high	1335:1338	high	1335:1338	Both unmodified chitosan and tetraethyl orthosilicate crosslinked composite membranes successfully separated feed mixture containing 4.6 wt% water by exhibiting water fluxes of 0.024 and 0.019 kg/m(2)h, whereas the corresponding selectivities were found to be as high as 182 and 225, respectively.
26572460	6	16	theme	corresponding	1287:1299	arg1	selectivities					1301:1313	the corresponding selectivities	1283:1313	the corresponding selectivities	1283:1313	Both unmodified chitosan and tetraethyl orthosilicate crosslinked composite membranes successfully separated feed mixture containing 4.6 wt% water by exhibiting water fluxes of 0.024 and 0.019 kg/m(2)h, whereas the corresponding selectivities were found to be as high as 182 and 225, respectively.
26572460	6	17	theme	unmodified	1077:1086	arg1	chitosan					1088:1095	unmodified chitosan	1077:1095	unmodified chitosan	1077:1095	Both unmodified chitosan and tetraethyl orthosilicate crosslinked composite membranes successfully separated feed mixture containing 4.6 wt% water by exhibiting water fluxes of 0.024 and 0.019 kg/m(2)h, whereas the corresponding selectivities were found to be as high as 182 and 225, respectively.
26572460	6	18	contain	containing	1194:1203	arg1	mixture					1186:1192	feed mixture	1181:1192	feed mixture containing 4.6 wt% water by exhibiting water fluxes of 0.024 and 0.019 kg/m(2)h	1181:1272	Both unmodified chitosan and tetraethyl orthosilicate crosslinked composite membranes successfully separated feed mixture containing 4.6 wt% water by exhibiting water fluxes of 0.024 and 0.019 kg/m(2)h, whereas the corresponding selectivities were found to be as high as 182 and 225, respectively.
26572460	6	18	contain	containing	1194:1203	arg2	water					1213:1217	4.6 wt% water	1205:1217	4.6 wt% water	1205:1217	Both unmodified chitosan and tetraethyl orthosilicate crosslinked composite membranes successfully separated feed mixture containing 4.6 wt% water by exhibiting water fluxes of 0.024 and 0.019 kg/m(2)h, whereas the corresponding selectivities were found to be as high as 182 and 225, respectively.
26572460	6	19	theme	tetraethyl	1101:1110	arg1	orthosilicate					1112:1124	tetraethyl orthosilicate	1101:1124	tetraethyl orthosilicate	1101:1124	Both unmodified chitosan and tetraethyl orthosilicate crosslinked composite membranes successfully separated feed mixture containing 4.6 wt% water by exhibiting water fluxes of 0.024 and 0.019 kg/m(2)h, whereas the corresponding selectivities were found to be as high as 182 and 225, respectively.
26572460	1	20	theme	chitosan	205:212	arg1	membrane					219:226	hydrophilic and solvent resistant chitosan (CS) membrane	171:226	hydrophilic and solvent resistant chitosan (CS) membrane on porous solvent resistant poly(ether-block-amide) (PEBA-2533) substrate	171:300	A novel composite barrier comprising of hydrophilic and solvent resistant chitosan (CS) membrane on porous solvent resistant poly(ether-block-amide) (PEBA-2533) substrate was synthesized for pervaporation (PV) based dehydration of the polar aprotic n-methyl-2-pyrolidone (NMP) green solvent.
26572460	1	21	theme	n-methyl-2-pyrolidone	380:400	arg1	solvent					414:420	the polar aprotic n-methyl-2-pyrolidone (NMP) green solvent	362:420	the polar aprotic n-methyl-2-pyrolidone (NMP) green solvent	362:420	A novel composite barrier comprising of hydrophilic and solvent resistant chitosan (CS) membrane on porous solvent resistant poly(ether-block-amide) (PEBA-2533) substrate was synthesized for pervaporation (PV) based dehydration of the polar aprotic n-methyl-2-pyrolidone (NMP) green solvent.
26572460	6	22	theme	composite	1138:1146	arg1	membranes					1148:1156	crosslinked composite membranes	1126:1156	crosslinked composite membranes	1126:1156	Both unmodified chitosan and tetraethyl orthosilicate crosslinked composite membranes successfully separated feed mixture containing 4.6 wt% water by exhibiting water fluxes of 0.024 and 0.019 kg/m(2)h, whereas the corresponding selectivities were found to be as high as 182 and 225, respectively.
26572460	5	23	theme	mixing	884:889	arg1	experiments					891:901	complete mixing experiments	875:901	complete mixing experiments	875:901	On the basis of complete mixing experiments, a numerical simulation was performed to predict membrane area requirement and exit streams' compositions for commercial pervaporation units operated in plug flow mode.
26572460	0	24	theme	resistant	8:16	arg1	chitosan/poly					18:30	Solvent resistant chitosan/poly	0:30	Solvent resistant chitosan/poly(ether-block-amide) composite membranes for pervaporation of n-methyl-2-pyrrolidone/water mixtures.	0:129	Solvent resistant chitosan/poly(ether-block-amide) composite membranes for pervaporation of n-methyl-2-pyrrolidone/water mixtures.
26572460	0	24	theme	resistant	8:16	arg1	ether-block-amide					32:48	ether-block-amide	32:48	ether-block-amide	32:48	Solvent resistant chitosan/poly(ether-block-amide) composite membranes for pervaporation of n-methyl-2-pyrrolidone/water mixtures.
26572460	6	25	theme	crosslinked	1126:1136	arg1	membranes					1148:1156	crosslinked composite membranes	1126:1156	crosslinked composite membranes	1126:1156	Both unmodified chitosan and tetraethyl orthosilicate crosslinked composite membranes successfully separated feed mixture containing 4.6 wt% water by exhibiting water fluxes of 0.024 and 0.019 kg/m(2)h, whereas the corresponding selectivities were found to be as high as 182 and 225, respectively.
26572460	5	26	theme	area	961:964	arg1	requirement					966:976	membrane area requirement	952:976	membrane area requirement	952:976	On the basis of complete mixing experiments, a numerical simulation was performed to predict membrane area requirement and exit streams' compositions for commercial pervaporation units operated in plug flow mode.
26572460	4	27	theme	finite	710:715	arg1	model					738:742	A two-dimensional finite element method (FEM) model	692:742	A two-dimensional finite element method (FEM) model	692:742	A two-dimensional finite element method (FEM) model was developed to predict the concentration profile within the membrane through computational fluid dynamics (CFD).
26572460	0	28	theme	Solvent	0:6	arg1	chitosan/poly					18:30	Solvent resistant chitosan/poly	0:30	Solvent resistant chitosan/poly(ether-block-amide) composite membranes for pervaporation of n-methyl-2-pyrrolidone/water mixtures.	0:129	Solvent resistant chitosan/poly(ether-block-amide) composite membranes for pervaporation of n-methyl-2-pyrrolidone/water mixtures.
26572460	0	28	theme	Solvent	0:6	arg1	ether-block-amide					32:48	ether-block-amide	32:48	ether-block-amide	32:48	Solvent resistant chitosan/poly(ether-block-amide) composite membranes for pervaporation of n-methyl-2-pyrrolidone/water mixtures.
26572460	5	29	theme	complete	875:882	arg1	experiments					891:901	complete mixing experiments	875:901	complete mixing experiments	875:901	On the basis of complete mixing experiments, a numerical simulation was performed to predict membrane area requirement and exit streams' compositions for commercial pervaporation units operated in plug flow mode.
26572460	4	30	theme	two-dimensional	694:708	arg1	model					738:742	A two-dimensional finite element method (FEM) model	692:742	A two-dimensional finite element method (FEM) model	692:742	A two-dimensional finite element method (FEM) model was developed to predict the concentration profile within the membrane through computational fluid dynamics (CFD).
26572460	3	31	theme	water	577:581	arg1	concentration					583:595	feed water concentration	572:595	feed water concentration	572:595	Operating parameters such as feed water concentration, permeate pressure and membrane thickness were varied to assess membrane flux and selectivity.
26572460	5	32	dep	experiments	891:901	arg1	basis					866:870	basis	866:870	basis	866:870	On the basis of complete mixing experiments, a numerical simulation was performed to predict membrane area requirement and exit streams' compositions for commercial pervaporation units operated in plug flow mode.
26572460	5	32	dep	experiments	891:901	arg1	the					862:864	the	862:864	the	862:864	On the basis of complete mixing experiments, a numerical simulation was performed to predict membrane area requirement and exit streams' compositions for commercial pervaporation units operated in plug flow mode.
26572460	0	33	theme	chitosan/poly	18:30	arg1	membranes					61:69	Solvent resistant chitosan/poly(ether-block-amide) composite membranes	0:69	Solvent resistant chitosan/poly(ether-block-amide) composite membranes for pervaporation of n-methyl-2-pyrrolidone/water mixtures.	0:129	Solvent resistant chitosan/poly(ether-block-amide) composite membranes for pervaporation of n-methyl-2-pyrrolidone/water mixtures.
26572460	6	34	theme	water	1233:1237	arg1	fluxes					1239:1244	water fluxes	1233:1244	water fluxes of 0.024 and 0.019 kg/m(2)h	1233:1272	Both unmodified chitosan and tetraethyl orthosilicate crosslinked composite membranes successfully separated feed mixture containing 4.6 wt% water by exhibiting water fluxes of 0.024 and 0.019 kg/m(2)h, whereas the corresponding selectivities were found to be as high as 182 and 225, respectively.
26572460	1	35	theme	NMP	403:405	arg1	solvent					414:420	the polar aprotic n-methyl-2-pyrolidone (NMP) green solvent	362:420	the polar aprotic n-methyl-2-pyrolidone (NMP) green solvent	362:420	A novel composite barrier comprising of hydrophilic and solvent resistant chitosan (CS) membrane on porous solvent resistant poly(ether-block-amide) (PEBA-2533) substrate was synthesized for pervaporation (PV) based dehydration of the polar aprotic n-methyl-2-pyrolidone (NMP) green solvent.
26572460	1	36	theme	novel	133:137	arg1	barrier					149:155	A novel composite barrier	131:155	A novel composite barrier comprising of hydrophilic and solvent resistant chitosan (CS) membrane on porous solvent resistant poly(ether-block-amide) (PEBA-2533) substrate	131:300	A novel composite barrier comprising of hydrophilic and solvent resistant chitosan (CS) membrane on porous solvent resistant poly(ether-block-amide) (PEBA-2533) substrate was synthesized for pervaporation (PV) based dehydration of the polar aprotic n-methyl-2-pyrolidone (NMP) green solvent.
26572460	2	37	theme	composite	427:435	arg1	barrier					437:443	The composite barrier	423:443	The composite barrier	423:443	The composite barrier was crosslinked with tetraethyl orthosilicate (TEOS) to control swelling and enhance selectivity.
26572460	3	38	theme	membrane	620:627	arg1	thickness					629:637	membrane thickness	620:637	membrane thickness	620:637	Operating parameters such as feed water concentration, permeate pressure and membrane thickness were varied to assess membrane flux and selectivity.
26572460	1	39	theme	porous	231:236	arg1	ether-block-amide					261:277	ether-block-amide	261:277	ether-block-amide	261:277	A novel composite barrier comprising of hydrophilic and solvent resistant chitosan (CS) membrane on porous solvent resistant poly(ether-block-amide) (PEBA-2533) substrate was synthesized for pervaporation (PV) based dehydration of the polar aprotic n-methyl-2-pyrolidone (NMP) green solvent.
26572460	1	39	theme	porous	231:236	arg1	PEBA-2533					281:289	PEBA-2533	281:289	PEBA-2533	281:289	A novel composite barrier comprising of hydrophilic and solvent resistant chitosan (CS) membrane on porous solvent resistant poly(ether-block-amide) (PEBA-2533) substrate was synthesized for pervaporation (PV) based dehydration of the polar aprotic n-methyl-2-pyrolidone (NMP) green solvent.
26572460	1	39	theme	porous	231:236	arg1	poly					256:259	porous solvent resistant poly	231:259	porous solvent resistant poly(ether-block-amide) (PEBA-2533) substrate	231:300	A novel composite barrier comprising of hydrophilic and solvent resistant chitosan (CS) membrane on porous solvent resistant poly(ether-block-amide) (PEBA-2533) substrate was synthesized for pervaporation (PV) based dehydration of the polar aprotic n-methyl-2-pyrolidone (NMP) green solvent.
26572460	3	40	dep	pressure	607:614	arg1	permeate					598:605	permeate	598:605	permeate	598:605	Operating parameters such as feed water concentration, permeate pressure and membrane thickness were varied to assess membrane flux and selectivity.
26572460	1	41	theme	green	408:412	arg1	solvent					414:420	the polar aprotic n-methyl-2-pyrolidone (NMP) green solvent	362:420	the polar aprotic n-methyl-2-pyrolidone (NMP) green solvent	362:420	A novel composite barrier comprising of hydrophilic and solvent resistant chitosan (CS) membrane on porous solvent resistant poly(ether-block-amide) (PEBA-2533) substrate was synthesized for pervaporation (PV) based dehydration of the polar aprotic n-methyl-2-pyrolidone (NMP) green solvent.
26572460	5	42	theme	plug	1056:1059	arg1	mode					1066:1069	plug flow mode	1056:1069	plug flow mode	1056:1069	On the basis of complete mixing experiments, a numerical simulation was performed to predict membrane area requirement and exit streams' compositions for commercial pervaporation units operated in plug flow mode.
26572460	1	43	theme	solvent	238:244	arg1	ether-block-amide					261:277	ether-block-amide	261:277	ether-block-amide	261:277	A novel composite barrier comprising of hydrophilic and solvent resistant chitosan (CS) membrane on porous solvent resistant poly(ether-block-amide) (PEBA-2533) substrate was synthesized for pervaporation (PV) based dehydration of the polar aprotic n-methyl-2-pyrolidone (NMP) green solvent.
26572460	1	43	theme	solvent	238:244	arg1	PEBA-2533					281:289	PEBA-2533	281:289	PEBA-2533	281:289	A novel composite barrier comprising of hydrophilic and solvent resistant chitosan (CS) membrane on porous solvent resistant poly(ether-block-amide) (PEBA-2533) substrate was synthesized for pervaporation (PV) based dehydration of the polar aprotic n-methyl-2-pyrolidone (NMP) green solvent.
26572460	1	43	theme	solvent	238:244	arg1	poly					256:259	porous solvent resistant poly	231:259	porous solvent resistant poly(ether-block-amide) (PEBA-2533) substrate	231:300	A novel composite barrier comprising of hydrophilic and solvent resistant chitosan (CS) membrane on porous solvent resistant poly(ether-block-amide) (PEBA-2533) substrate was synthesized for pervaporation (PV) based dehydration of the polar aprotic n-methyl-2-pyrolidone (NMP) green solvent.
26572460	1	44	theme	solvent	414:420	arg1	dehydration					347:357	dehydration	347:357	dehydration of the polar aprotic n-methyl-2-pyrolidone (NMP) green solvent	347:420	A novel composite barrier comprising of hydrophilic and solvent resistant chitosan (CS) membrane on porous solvent resistant poly(ether-block-amide) (PEBA-2533) substrate was synthesized for pervaporation (PV) based dehydration of the polar aprotic n-methyl-2-pyrolidone (NMP) green solvent.
26572460	5	45	theme	flow	1061:1064	arg1	mode					1066:1069	plug flow mode	1056:1069	plug flow mode	1056:1069	On the basis of complete mixing experiments, a numerical simulation was performed to predict membrane area requirement and exit streams' compositions for commercial pervaporation units operated in plug flow mode.
26572460	1	46	theme	composite	139:147	arg1	barrier					149:155	A novel composite barrier	131:155	A novel composite barrier comprising of hydrophilic and solvent resistant chitosan (CS) membrane on porous solvent resistant poly(ether-block-amide) (PEBA-2533) substrate	131:300	A novel composite barrier comprising of hydrophilic and solvent resistant chitosan (CS) membrane on porous solvent resistant poly(ether-block-amide) (PEBA-2533) substrate was synthesized for pervaporation (PV) based dehydration of the polar aprotic n-methyl-2-pyrolidone (NMP) green solvent.
26572460	0	47	theme	composite	51:59	arg1	membranes					61:69	Solvent resistant chitosan/poly(ether-block-amide) composite membranes	0:69	Solvent resistant chitosan/poly(ether-block-amide) composite membranes for pervaporation of n-methyl-2-pyrrolidone/water mixtures.	0:129	Solvent resistant chitosan/poly(ether-block-amide) composite membranes for pervaporation of n-methyl-2-pyrrolidone/water mixtures.
26572460	6	48	theme	%	1211:1211	arg1	water					1213:1217	4.6 wt% water	1205:1217	4.6 wt% water	1205:1217	Both unmodified chitosan and tetraethyl orthosilicate crosslinked composite membranes successfully separated feed mixture containing 4.6 wt% water by exhibiting water fluxes of 0.024 and 0.019 kg/m(2)h, whereas the corresponding selectivities were found to be as high as 182 and 225, respectively.
26572460	1	49	from	membrane	219:226	arg1	substrate					292:300	porous solvent resistant poly(ether-block-amide) (PEBA-2533) substrate	231:300	porous solvent resistant poly(ether-block-amide) (PEBA-2533) substrate	231:300	A novel composite barrier comprising of hydrophilic and solvent resistant chitosan (CS) membrane on porous solvent resistant poly(ether-block-amide) (PEBA-2533) substrate was synthesized for pervaporation (PV) based dehydration of the polar aprotic n-methyl-2-pyrolidone (NMP) green solvent.
26572460	3	50	theme	membrane	661:668	arg1	flux					670:673	membrane flux	661:673	membrane flux	661:673	Operating parameters such as feed water concentration, permeate pressure and membrane thickness were varied to assess membrane flux and selectivity.
26572460	5	51	theme	commercial	1013:1022	arg1	units					1038:1042	commercial pervaporation units	1013:1042	commercial pervaporation units operated in plug flow mode	1013:1069	On the basis of complete mixing experiments, a numerical simulation was performed to predict membrane area requirement and exit streams' compositions for commercial pervaporation units operated in plug flow mode.
26572460	4	52	theme	computational	823:835	arg1	CFD					853:855	CFD	853:855	CFD	853:855	A two-dimensional finite element method (FEM) model was developed to predict the concentration profile within the membrane through computational fluid dynamics (CFD).
26572460	4	52	theme	computational	823:835	arg1	dynamics					843:850	computational fluid dynamics	823:850	computational fluid dynamics (CFD)	823:856	A two-dimensional finite element method (FEM) model was developed to predict the concentration profile within the membrane through computational fluid dynamics (CFD).
26572460	4	53	theme	element	717:723	arg1	model					738:742	A two-dimensional finite element method (FEM) model	692:742	A two-dimensional finite element method (FEM) model	692:742	A two-dimensional finite element method (FEM) model was developed to predict the concentration profile within the membrane through computational fluid dynamics (CFD).
26572460	4	54	theme	concentration	773:785	arg1	profile					787:793	the concentration profile	769:793	the concentration profile	769:793	A two-dimensional finite element method (FEM) model was developed to predict the concentration profile within the membrane through computational fluid dynamics (CFD).
26572460	1	55	theme	resistant	246:254	arg1	ether-block-amide					261:277	ether-block-amide	261:277	ether-block-amide	261:277	A novel composite barrier comprising of hydrophilic and solvent resistant chitosan (CS) membrane on porous solvent resistant poly(ether-block-amide) (PEBA-2533) substrate was synthesized for pervaporation (PV) based dehydration of the polar aprotic n-methyl-2-pyrolidone (NMP) green solvent.
26572460	1	55	theme	resistant	246:254	arg1	PEBA-2533					281:289	PEBA-2533	281:289	PEBA-2533	281:289	A novel composite barrier comprising of hydrophilic and solvent resistant chitosan (CS) membrane on porous solvent resistant poly(ether-block-amide) (PEBA-2533) substrate was synthesized for pervaporation (PV) based dehydration of the polar aprotic n-methyl-2-pyrolidone (NMP) green solvent.
26572460	1	55	theme	resistant	246:254	arg1	poly					256:259	porous solvent resistant poly	231:259	porous solvent resistant poly(ether-block-amide) (PEBA-2533) substrate	231:300	A novel composite barrier comprising of hydrophilic and solvent resistant chitosan (CS) membrane on porous solvent resistant poly(ether-block-amide) (PEBA-2533) substrate was synthesized for pervaporation (PV) based dehydration of the polar aprotic n-methyl-2-pyrolidone (NMP) green solvent.
26572460	6	56	theme	feed	1181:1184	arg1	mixture					1186:1192	feed mixture	1181:1192	feed mixture containing 4.6 wt% water by exhibiting water fluxes of 0.024 and 0.019 kg/m(2)h	1181:1272	Both unmodified chitosan and tetraethyl orthosilicate crosslinked composite membranes successfully separated feed mixture containing 4.6 wt% water by exhibiting water fluxes of 0.024 and 0.019 kg/m(2)h, whereas the corresponding selectivities were found to be as high as 182 and 225, respectively.
26572460	5	57	theme	pervaporation	1024:1036	arg1	units					1038:1042	commercial pervaporation units	1013:1042	commercial pervaporation units operated in plug flow mode	1013:1069	On the basis of complete mixing experiments, a numerical simulation was performed to predict membrane area requirement and exit streams' compositions for commercial pervaporation units operated in plug flow mode.
26572460	3	58	theme	Operating	543:551	arg1	concentration					583:595	feed water concentration	572:595	feed water concentration	572:595	Operating parameters such as feed water concentration, permeate pressure and membrane thickness were varied to assess membrane flux and selectivity.
26572460	3	58	theme	Operating	543:551	arg1	parameters					553:562	Operating parameters	543:562	Operating parameters such as feed water concentration, permeate pressure and membrane thickness	543:637	Operating parameters such as feed water concentration, permeate pressure and membrane thickness were varied to assess membrane flux and selectivity.
26572460	3	58	theme	Operating	543:551	arg1	thickness					629:637	membrane thickness	620:637	membrane thickness	620:637	Operating parameters such as feed water concentration, permeate pressure and membrane thickness were varied to assess membrane flux and selectivity.
26572460	3	58	theme	Operating	543:551	arg1	pressure					607:614	permeate pressure	598:614	permeate pressure	598:614	Operating parameters such as feed water concentration, permeate pressure and membrane thickness were varied to assess membrane flux and selectivity.
26572460	1	59	theme	poly	256:259	arg1	substrate					292:300	porous solvent resistant poly(ether-block-amide) (PEBA-2533) substrate	231:300	porous solvent resistant poly(ether-block-amide) (PEBA-2533) substrate	231:300	A novel composite barrier comprising of hydrophilic and solvent resistant chitosan (CS) membrane on porous solvent resistant poly(ether-block-amide) (PEBA-2533) substrate was synthesized for pervaporation (PV) based dehydration of the polar aprotic n-methyl-2-pyrolidone (NMP) green solvent.
26572460	1	60	theme	hydrophilic	171:181	arg1	CS					215:216	CS	215:216	CS	215:216	A novel composite barrier comprising of hydrophilic and solvent resistant chitosan (CS) membrane on porous solvent resistant poly(ether-block-amide) (PEBA-2533) substrate was synthesized for pervaporation (PV) based dehydration of the polar aprotic n-methyl-2-pyrolidone (NMP) green solvent.
26572460	1	60	theme	hydrophilic	171:181	arg1	chitosan					205:212	hydrophilic and solvent resistant chitosan	171:212	hydrophilic and solvent resistant chitosan (CS) membrane on porous solvent resistant poly(ether-block-amide) (PEBA-2533) substrate	171:300	A novel composite barrier comprising of hydrophilic and solvent resistant chitosan (CS) membrane on porous solvent resistant poly(ether-block-amide) (PEBA-2533) substrate was synthesized for pervaporation (PV) based dehydration of the polar aprotic n-methyl-2-pyrolidone (NMP) green solvent.
26572460	4	61	theme	fluid	837:841	arg1	CFD					853:855	CFD	853:855	CFD	853:855	A two-dimensional finite element method (FEM) model was developed to predict the concentration profile within the membrane through computational fluid dynamics (CFD).
26572460	4	61	theme	fluid	837:841	arg1	dynamics					843:850	computational fluid dynamics	823:850	computational fluid dynamics (CFD)	823:856	A two-dimensional finite element method (FEM) model was developed to predict the concentration profile within the membrane through computational fluid dynamics (CFD).
26572460	2	62	theme	tetraethyl	466:475	arg1	TEOS					492:495	TEOS	492:495	TEOS	492:495	The composite barrier was crosslinked with tetraethyl orthosilicate (TEOS) to control swelling and enhance selectivity.
26572460	2	62	theme	tetraethyl	466:475	arg1	orthosilicate					477:489	tetraethyl orthosilicate	466:489	tetraethyl orthosilicate (TEOS)	466:496	The composite barrier was crosslinked with tetraethyl orthosilicate (TEOS) to control swelling and enhance selectivity.
24655156	1	0	theme	spoilage	356:363	arg1	yeasts					365:370	typical spoilage yeasts	348:370	typical spoilage yeasts	348:370	AIMS To investigate the effect of anionic polysaccharides often used in beverage applications (xanthan and λ-carrageenan) on the antimicrobial efficacy of the cationic surfactant lauric arginate (LAE) against typical spoilage yeasts.
24655156	7	1	theme	varying	1230:1236	arg1	charges					1249:1255	varying electrical charges	1230:1255	varying electrical charges	1230:1255	Electrophoresis measurements showed that complexes had varying electrical charges and dimensions depending on solution composition.
24655156	1	2	theme	anionic	173:179	arg1	polysaccharides					181:195	anionic polysaccharides	173:195	anionic polysaccharides often used in beverage applications (xanthan and λ-carrageenan)	173:259	AIMS To investigate the effect of anionic polysaccharides often used in beverage applications (xanthan and λ-carrageenan) on the antimicrobial efficacy of the cationic surfactant lauric arginate (LAE) against typical spoilage yeasts.
24655156	1	3	theme	antimicrobial	268:280	arg1	efficacy					282:289	the antimicrobial efficacy	264:289	the antimicrobial efficacy of the cationic surfactant lauric arginate (LAE) against typical spoilage yeasts	264:370	AIMS To investigate the effect of anionic polysaccharides often used in beverage applications (xanthan and λ-carrageenan) on the antimicrobial efficacy of the cationic surfactant lauric arginate (LAE) against typical spoilage yeasts.
24655156	0	4	theme	antimicrobial	91:103	arg1	activity					105:112	antimicrobial activity	91:112	antimicrobial activity against spoilage yeasts	91:136	Electrostatic interactions of cationic lauric arginate with anionic polysaccharides affect antimicrobial activity against spoilage yeasts.
24655156	7	5	theme	electrical	1238:1247	arg1	charges					1249:1255	varying electrical charges	1230:1255	varying electrical charges	1230:1255	Electrophoresis measurements showed that complexes had varying electrical charges and dimensions depending on solution composition.
24655156	1	6	theme	polysaccharides	181:195	arg1	effect					163:168	the effect	159:168	the effect of anionic polysaccharides often used in beverage applications (xanthan and λ-carrageenan) on the antimicrobial efficacy of the cationic surfactant lauric arginate (LAE) against typical spoilage yeasts	159:370	AIMS To investigate the effect of anionic polysaccharides often used in beverage applications (xanthan and λ-carrageenan) on the antimicrobial efficacy of the cationic surfactant lauric arginate (LAE) against typical spoilage yeasts.
24655156	6	7	theme	sedimentation	1066:1078	arg1	measurements					1080:1091	Turbidity and sedimentation measurements	1052:1091	measurements	1080:1091	Turbidity and sedimentation measurements indicated that complexes between LAE and anionic polysaccharides had been formed.
24655156	0	8	theme	spoilage	122:129	arg1	yeasts					131:136	spoilage yeasts	122:136	spoilage yeasts	122:136	Electrostatic interactions of cationic lauric arginate with anionic polysaccharides affect antimicrobial activity against spoilage yeasts.
24655156	3	9	theme	minimal	743:749	arg1	concentrations					773:786	the minimal inhibitory and lethal concentrations	739:786	the minimal inhibitory and lethal concentrations (MIC and MLC)	739:800	Combining LAE with either xanthan or λ-carrageenan caused a pronounced decrease in LAE's antimicrobial efficacy, with the minimal inhibitory and lethal concentrations (MIC and MLC) both increasing with increasing polysaccharide concentration.
24655156	3	9	theme	minimal	743:749	arg1	MLC					797:799	MLC	797:799	MLC	797:799	Combining LAE with either xanthan or λ-carrageenan caused a pronounced decrease in LAE's antimicrobial efficacy, with the minimal inhibitory and lethal concentrations (MIC and MLC) both increasing with increasing polysaccharide concentration.
24655156	3	9	theme	minimal	743:749	arg1	MIC					789:791	MIC	789:791	MIC	789:791	Combining LAE with either xanthan or λ-carrageenan caused a pronounced decrease in LAE's antimicrobial efficacy, with the minimal inhibitory and lethal concentrations (MIC and MLC) both increasing with increasing polysaccharide concentration.
24655156	2	10	dep	absence	514:520	arg1	the					510:512	the	510:512	the	510:512	METHODS AND RESULTS The antimicrobial efficacy of LAE against Saccharomyces cerevisiae, Candida albicans and Zygosaccharomyces bailii in the absence and presence of anionic polysaccharides was assessed by microtitre and macrobroth dilution assays.
24655156	9	11	from	agent	1601:1605	arg1	products					1628:1635	beverage and food products	1610:1635	products	1628:1635	SIGNIFICANCE AND IMPACT OF THE STUDY Results have important implications for the utilization of LAE as an antimicrobial agent in beverage and food products containing anionic polysaccharides.
24655156	5	12	theme	solutions	1027:1035	arg1	properties					1013:1022	physical properties	1004:1022	physical properties of solutions	1004:1035	To determine the cause of loss of activity, physical properties of solutions were examined.
24655156	9	13	theme	LAE	1577:1579	arg1	utilization					1562:1572	the utilization	1558:1572	the utilization of LAE	1558:1579	SIGNIFICANCE AND IMPACT OF THE STUDY Results have important implications for the utilization of LAE as an antimicrobial agent in beverage and food products containing anionic polysaccharides.
24655156	9	14	contain	have	1526:1529	arg1	IMPACT					1498:1503	IMPACT	1498:1503	IMPACT	1498:1503	SIGNIFICANCE AND IMPACT OF THE STUDY Results have important implications for the utilization of LAE as an antimicrobial agent in beverage and food products containing anionic polysaccharides.
24655156	9	14	contain	have	1526:1529	arg1	SIGNIFICANCE					1481:1492	SIGNIFICANCE	1481:1492	SIGNIFICANCE	1481:1492	SIGNIFICANCE AND IMPACT OF THE STUDY Results have important implications for the utilization of LAE as an antimicrobial agent in beverage and food products containing anionic polysaccharides.
24655156	9	14	contain	have	1526:1529	arg2	implications					1541:1552	important implications	1531:1552	important implications for the utilization of LAE	1531:1579	SIGNIFICANCE AND IMPACT OF THE STUDY Results have important implications for the utilization of LAE as an antimicrobial agent in beverage and food products containing anionic polysaccharides.
24655156	9	14	contain	have	1526:1529	arg2	agent					1601:1605	an antimicrobial agent	1584:1605	an antimicrobial agent in beverage and food products containing anionic polysaccharides	1584:1670	SIGNIFICANCE AND IMPACT OF THE STUDY Results have important implications for the utilization of LAE as an antimicrobial agent in beverage and food products containing anionic polysaccharides.
24655156	4	15	theme	λ-carrageenan	945:957	arg1	addition					933:940	the addition	929:940	the addition of λ-carrageenan	929:957	This reduction in antimicrobial efficacy was more pronounced for the addition of λ-carrageenan.
24655156	1	16	from	effect	163:168	arg1	efficacy					282:289	the antimicrobial efficacy	264:289	the antimicrobial efficacy of the cationic surfactant lauric arginate (LAE) against typical spoilage yeasts	264:370	AIMS To investigate the effect of anionic polysaccharides often used in beverage applications (xanthan and λ-carrageenan) on the antimicrobial efficacy of the cationic surfactant lauric arginate (LAE) against typical spoilage yeasts.
24655156	2	17	dep	METHODS	373:379	arg1	assessed					566:573	assessed	566:573	was assessed by microtitre and macrobroth dilution assays	562:618	METHODS AND RESULTS The antimicrobial efficacy of LAE against Saccharomyces cerevisiae, Candida albicans and Zygosaccharomyces bailii in the absence and presence of anionic polysaccharides was assessed by microtitre and macrobroth dilution assays.
24655156	9	18	theme	STUDY	1512:1516	arg1	Results					1518:1524	THE STUDY Results	1508:1524	THE STUDY Results	1508:1524	SIGNIFICANCE AND IMPACT OF THE STUDY Results have important implications for the utilization of LAE as an antimicrobial agent in beverage and food products containing anionic polysaccharides.
24655156	6	19	theme	Turbidity	1052:1060	arg1	measurements					1080:1091	Turbidity and sedimentation measurements	1052:1091	measurements	1080:1091	Turbidity and sedimentation measurements indicated that complexes between LAE and anionic polysaccharides had been formed.
24655156	8	20	theme	complex	1427:1433	arg1	formation					1435:1443	complex formation	1427:1443	complex formation	1427:1443	CONCLUSION Results suggest that electrostatic interactions between LAE and anionic polysaccharides play a major role in complex formation and loss of antimicrobial activity.
24655156	2	21	theme	LAE	423:425	arg1	efficacy					411:418	The antimicrobial efficacy	393:418	The antimicrobial efficacy of LAE against Saccharomyces cerevisiae, Candida albicans and Zygosaccharomyces bailii in the absence and presence of anionic polysaccharides	393:560	METHODS AND RESULTS The antimicrobial efficacy of LAE against Saccharomyces cerevisiae, Candida albicans and Zygosaccharomyces bailii in the absence and presence of anionic polysaccharides was assessed by microtitre and macrobroth dilution assays.
24655156	2	22	theme	polysaccharides	546:560	arg1	presence					526:533	presence	526:533	presence	526:533	METHODS AND RESULTS The antimicrobial efficacy of LAE against Saccharomyces cerevisiae, Candida albicans and Zygosaccharomyces bailii in the absence and presence of anionic polysaccharides was assessed by microtitre and macrobroth dilution assays.
24655156	2	22	theme	polysaccharides	546:560	arg1	absence					514:520	absence	514:520	absence	514:520	METHODS AND RESULTS The antimicrobial efficacy of LAE against Saccharomyces cerevisiae, Candida albicans and Zygosaccharomyces bailii in the absence and presence of anionic polysaccharides was assessed by microtitre and macrobroth dilution assays.
24655156	3	23	theme	lethal	766:771	arg1	concentrations					773:786	the minimal inhibitory and lethal concentrations	739:786	the minimal inhibitory and lethal concentrations (MIC and MLC)	739:800	Combining LAE with either xanthan or λ-carrageenan caused a pronounced decrease in LAE's antimicrobial efficacy, with the minimal inhibitory and lethal concentrations (MIC and MLC) both increasing with increasing polysaccharide concentration.
24655156	3	23	theme	lethal	766:771	arg1	MLC					797:799	MLC	797:799	MLC	797:799	Combining LAE with either xanthan or λ-carrageenan caused a pronounced decrease in LAE's antimicrobial efficacy, with the minimal inhibitory and lethal concentrations (MIC and MLC) both increasing with increasing polysaccharide concentration.
24655156	3	23	theme	lethal	766:771	arg1	MIC					789:791	MIC	789:791	MIC	789:791	Combining LAE with either xanthan or λ-carrageenan caused a pronounced decrease in LAE's antimicrobial efficacy, with the minimal inhibitory and lethal concentrations (MIC and MLC) both increasing with increasing polysaccharide concentration.
24655156	8	24	theme	CONCLUSION	1307:1316	arg1	Results					1318:1324	CONCLUSION Results	1307:1324	CONCLUSION Results	1307:1324	CONCLUSION Results suggest that electrostatic interactions between LAE and anionic polysaccharides play a major role in complex formation and loss of antimicrobial activity.
24655156	9	25	theme	antimicrobial	1587:1599	arg1	agent					1601:1605	an antimicrobial agent	1584:1605	an antimicrobial agent in beverage and food products containing anionic polysaccharides	1584:1670	SIGNIFICANCE AND IMPACT OF THE STUDY Results have important implications for the utilization of LAE as an antimicrobial agent in beverage and food products containing anionic polysaccharides.
24655156	9	25	theme	antimicrobial	1587:1599	arg1	implications					1541:1552	important implications	1531:1552	important implications for the utilization of LAE	1531:1579	SIGNIFICANCE AND IMPACT OF THE STUDY Results have important implications for the utilization of LAE as an antimicrobial agent in beverage and food products containing anionic polysaccharides.
24655156	7	26	theme	Electrophoresis	1175:1189	arg1	measurements					1191:1202	Electrophoresis measurements	1175:1202	Electrophoresis measurements	1175:1202	Electrophoresis measurements showed that complexes had varying electrical charges and dimensions depending on solution composition.
24655156	9	27	theme	anionic	1648:1654	arg1	polysaccharides					1656:1670	anionic polysaccharides	1648:1670	anionic polysaccharides	1648:1670	SIGNIFICANCE AND IMPACT OF THE STUDY Results have important implications for the utilization of LAE as an antimicrobial agent in beverage and food products containing anionic polysaccharides.
24655156	0	28	theme	Electrostatic	0:12	arg1	interactions					14:25	Electrostatic interactions	0:25	Electrostatic interactions of cationic lauric arginate with anionic polysaccharides	0:82	Electrostatic interactions of cationic lauric arginate with anionic polysaccharides affect antimicrobial activity against spoilage yeasts.
24655156	1	29	theme	cationic	298:305	arg1	LAE					335:337	LAE	335:337	LAE	335:337	AIMS To investigate the effect of anionic polysaccharides often used in beverage applications (xanthan and λ-carrageenan) on the antimicrobial efficacy of the cationic surfactant lauric arginate (LAE) against typical spoilage yeasts.
24655156	1	29	theme	cationic	298:305	arg1	arginate					325:332	the cationic surfactant lauric arginate	294:332	the cationic surfactant lauric arginate (LAE)	294:338	AIMS To investigate the effect of anionic polysaccharides often used in beverage applications (xanthan and λ-carrageenan) on the antimicrobial efficacy of the cationic surfactant lauric arginate (LAE) against typical spoilage yeasts.
24655156	8	30	theme	anionic	1382:1388	arg1	polysaccharides					1390:1404	anionic polysaccharides	1382:1404	anionic polysaccharides	1382:1404	CONCLUSION Results suggest that electrostatic interactions between LAE and anionic polysaccharides play a major role in complex formation and loss of antimicrobial activity.
24655156	5	31	theme	physical	1004:1011	arg1	properties					1013:1022	physical properties	1004:1022	physical properties of solutions	1004:1035	To determine the cause of loss of activity, physical properties of solutions were examined.
24655156	1	32	theme	beverage	211:218	arg1	λ-carrageenan					246:258	λ-carrageenan	246:258	λ-carrageenan	246:258	AIMS To investigate the effect of anionic polysaccharides often used in beverage applications (xanthan and λ-carrageenan) on the antimicrobial efficacy of the cationic surfactant lauric arginate (LAE) against typical spoilage yeasts.
24655156	1	32	theme	beverage	211:218	arg1	xanthan					234:240	xanthan	234:240	xanthan	234:240	AIMS To investigate the effect of anionic polysaccharides often used in beverage applications (xanthan and λ-carrageenan) on the antimicrobial efficacy of the cationic surfactant lauric arginate (LAE) against typical spoilage yeasts.
24655156	1	32	theme	beverage	211:218	arg1	applications					220:231	beverage applications	211:231	beverage applications (xanthan and λ-carrageenan)	211:259	AIMS To investigate the effect of anionic polysaccharides often used in beverage applications (xanthan and λ-carrageenan) on the antimicrobial efficacy of the cationic surfactant lauric arginate (LAE) against typical spoilage yeasts.
24655156	0	33	theme	cationic	30:37	arg1	arginate					46:53	cationic lauric arginate	30:53	cationic lauric arginate	30:53	Electrostatic interactions of cationic lauric arginate with anionic polysaccharides affect antimicrobial activity against spoilage yeasts.
24655156	1	34	theme	surfactant	307:316	arg1	LAE					335:337	LAE	335:337	LAE	335:337	AIMS To investigate the effect of anionic polysaccharides often used in beverage applications (xanthan and λ-carrageenan) on the antimicrobial efficacy of the cationic surfactant lauric arginate (LAE) against typical spoilage yeasts.
24655156	1	34	theme	surfactant	307:316	arg1	arginate					325:332	the cationic surfactant lauric arginate	294:332	the cationic surfactant lauric arginate (LAE)	294:338	AIMS To investigate the effect of anionic polysaccharides often used in beverage applications (xanthan and λ-carrageenan) on the antimicrobial efficacy of the cationic surfactant lauric arginate (LAE) against typical spoilage yeasts.
24655156	2	35	theme	dilution	604:611	arg1	assays					613:618	macrobroth dilution assays	593:618	macrobroth dilution assays	593:618	METHODS AND RESULTS The antimicrobial efficacy of LAE against Saccharomyces cerevisiae, Candida albicans and Zygosaccharomyces bailii in the absence and presence of anionic polysaccharides was assessed by microtitre and macrobroth dilution assays.
24655156	8	36	theme	major	1413:1417	arg1	role					1419:1422	a major role	1411:1422	a major role	1411:1422	CONCLUSION Results suggest that electrostatic interactions between LAE and anionic polysaccharides play a major role in complex formation and loss of antimicrobial activity.
24655156	2	37	theme	Candida	461:467	arg1	albicans					469:476	Candida albicans	461:476	Candida albicans	461:476	METHODS AND RESULTS The antimicrobial efficacy of LAE against Saccharomyces cerevisiae, Candida albicans and Zygosaccharomyces bailii in the absence and presence of anionic polysaccharides was assessed by microtitre and macrobroth dilution assays.
24655156	1	38	theme	lauric	318:323	arg1	LAE					335:337	LAE	335:337	LAE	335:337	AIMS To investigate the effect of anionic polysaccharides often used in beverage applications (xanthan and λ-carrageenan) on the antimicrobial efficacy of the cationic surfactant lauric arginate (LAE) against typical spoilage yeasts.
24655156	1	38	theme	lauric	318:323	arg1	arginate					325:332	the cationic surfactant lauric arginate	294:332	the cationic surfactant lauric arginate (LAE)	294:338	AIMS To investigate the effect of anionic polysaccharides often used in beverage applications (xanthan and λ-carrageenan) on the antimicrobial efficacy of the cationic surfactant lauric arginate (LAE) against typical spoilage yeasts.
24655156	2	39	theme	macrobroth	593:602	arg1	assays					613:618	macrobroth dilution assays	593:618	macrobroth dilution assays	593:618	METHODS AND RESULTS The antimicrobial efficacy of LAE against Saccharomyces cerevisiae, Candida albicans and Zygosaccharomyces bailii in the absence and presence of anionic polysaccharides was assessed by microtitre and macrobroth dilution assays.
24655156	8	40	theme	activity	1471:1478	arg1	loss					1449:1452	loss	1449:1452	loss of antimicrobial activity	1449:1478	CONCLUSION Results suggest that electrostatic interactions between LAE and anionic polysaccharides play a major role in complex formation and loss of antimicrobial activity.
24655156	8	40	theme	activity	1471:1478	arg1	formation					1435:1443	complex formation	1427:1443	complex formation	1427:1443	CONCLUSION Results suggest that electrostatic interactions between LAE and anionic polysaccharides play a major role in complex formation and loss of antimicrobial activity.
24655156	6	41	theme	anionic	1134:1140	arg1	polysaccharides					1142:1156	anionic polysaccharides	1134:1156	anionic polysaccharides	1134:1156	Turbidity and sedimentation measurements indicated that complexes between LAE and anionic polysaccharides had been formed.
24655156	0	42	theme	arginate	46:53	arg1	interactions					14:25	Electrostatic interactions	0:25	Electrostatic interactions of cationic lauric arginate with anionic polysaccharides	0:82	Electrostatic interactions of cationic lauric arginate with anionic polysaccharides affect antimicrobial activity against spoilage yeasts.
24655156	1	43	theme	arginate	325:332	arg1	efficacy					282:289	the antimicrobial efficacy	264:289	the antimicrobial efficacy of the cationic surfactant lauric arginate (LAE) against typical spoilage yeasts	264:370	AIMS To investigate the effect of anionic polysaccharides often used in beverage applications (xanthan and λ-carrageenan) on the antimicrobial efficacy of the cationic surfactant lauric arginate (LAE) against typical spoilage yeasts.
24655156	4	44	theme	antimicrobial	882:894	arg1	efficacy					896:903	antimicrobial efficacy	882:903	antimicrobial efficacy	882:903	This reduction in antimicrobial efficacy was more pronounced for the addition of λ-carrageenan.
24655156	2	45	theme	antimicrobial	397:409	arg1	efficacy					411:418	The antimicrobial efficacy	393:418	The antimicrobial efficacy of LAE against Saccharomyces cerevisiae, Candida albicans and Zygosaccharomyces bailii in the absence and presence of anionic polysaccharides	393:560	METHODS AND RESULTS The antimicrobial efficacy of LAE against Saccharomyces cerevisiae, Candida albicans and Zygosaccharomyces bailii in the absence and presence of anionic polysaccharides was assessed by microtitre and macrobroth dilution assays.
24655156	9	46	theme	food	1623:1626	arg1	products					1628:1635	beverage and food products	1610:1635	products	1628:1635	SIGNIFICANCE AND IMPACT OF THE STUDY Results have important implications for the utilization of LAE as an antimicrobial agent in beverage and food products containing anionic polysaccharides.
24655156	0	47	theme	lauric	39:44	arg1	arginate					46:53	cationic lauric arginate	30:53	cationic lauric arginate	30:53	Electrostatic interactions of cationic lauric arginate with anionic polysaccharides affect antimicrobial activity against spoilage yeasts.
24655156	8	48	theme	electrostatic	1339:1351	arg1	interactions					1353:1364	electrostatic interactions	1339:1364	electrostatic interactions between LAE and anionic polysaccharides	1339:1404	CONCLUSION Results suggest that electrostatic interactions between LAE and anionic polysaccharides play a major role in complex formation and loss of antimicrobial activity.
24655156	0	49	theme	anionic	60:66	arg1	polysaccharides					68:82	anionic polysaccharides	60:82	anionic polysaccharides	60:82	Electrostatic interactions of cationic lauric arginate with anionic polysaccharides affect antimicrobial activity against spoilage yeasts.
24655156	3	50	theme	polysaccharide	834:847	arg1	concentration					849:861	polysaccharide concentration	834:861	polysaccharide concentration	834:861	Combining LAE with either xanthan or λ-carrageenan caused a pronounced decrease in LAE's antimicrobial efficacy, with the minimal inhibitory and lethal concentrations (MIC and MLC) both increasing with increasing polysaccharide concentration.
24655156	3	51	dep	increasing	823:832	arg1	increasing					807:816	increasing	807:816	increasing	807:816	Combining LAE with either xanthan or λ-carrageenan caused a pronounced decrease in LAE's antimicrobial efficacy, with the minimal inhibitory and lethal concentrations (MIC and MLC) both increasing with increasing polysaccharide concentration.
24655156	9	52	contain	containing	1637:1646	arg2	polysaccharides					1656:1670	anionic polysaccharides	1648:1670	anionic polysaccharides	1648:1670	SIGNIFICANCE AND IMPACT OF THE STUDY Results have important implications for the utilization of LAE as an antimicrobial agent in beverage and food products containing anionic polysaccharides.
24655156	9	52	contain	containing	1637:1646	arg1	products					1628:1635	beverage and food products	1610:1635	products	1628:1635	SIGNIFICANCE AND IMPACT OF THE STUDY Results have important implications for the utilization of LAE as an antimicrobial agent in beverage and food products containing anionic polysaccharides.
24655156	9	53	theme	Results	1518:1524	arg1	IMPACT					1498:1503	IMPACT	1498:1503	IMPACT	1498:1503	SIGNIFICANCE AND IMPACT OF THE STUDY Results have important implications for the utilization of LAE as an antimicrobial agent in beverage and food products containing anionic polysaccharides.
24655156	9	53	theme	Results	1518:1524	arg1	SIGNIFICANCE					1481:1492	SIGNIFICANCE	1481:1492	SIGNIFICANCE	1481:1492	SIGNIFICANCE AND IMPACT OF THE STUDY Results have important implications for the utilization of LAE as an antimicrobial agent in beverage and food products containing anionic polysaccharides.
24655156	3	54	theme	inhibitory	751:760	arg1	concentrations					773:786	the minimal inhibitory and lethal concentrations	739:786	the minimal inhibitory and lethal concentrations (MIC and MLC)	739:800	Combining LAE with either xanthan or λ-carrageenan caused a pronounced decrease in LAE's antimicrobial efficacy, with the minimal inhibitory and lethal concentrations (MIC and MLC) both increasing with increasing polysaccharide concentration.
24655156	3	54	theme	inhibitory	751:760	arg1	MLC					797:799	MLC	797:799	MLC	797:799	Combining LAE with either xanthan or λ-carrageenan caused a pronounced decrease in LAE's antimicrobial efficacy, with the minimal inhibitory and lethal concentrations (MIC and MLC) both increasing with increasing polysaccharide concentration.
24655156	3	54	theme	inhibitory	751:760	arg1	MIC					789:791	MIC	789:791	MIC	789:791	Combining LAE with either xanthan or λ-carrageenan caused a pronounced decrease in LAE's antimicrobial efficacy, with the minimal inhibitory and lethal concentrations (MIC and MLC) both increasing with increasing polysaccharide concentration.
24655156	0	55	with	interactions	14:25	arg1	polysaccharides					68:82	anionic polysaccharides	60:82	anionic polysaccharides	60:82	Electrostatic interactions of cationic lauric arginate with anionic polysaccharides affect antimicrobial activity against spoilage yeasts.
24655156	5	56	theme	loss	986:989	arg1	cause					977:981	the cause	973:981	the cause of loss of activity	973:1001	To determine the cause of loss of activity, physical properties of solutions were examined.
24655156	3	57	from	decrease	692:699	arg1	efficacy					724:731	LAE's antimicrobial efficacy	704:731	LAE's antimicrobial efficacy	704:731	Combining LAE with either xanthan or λ-carrageenan caused a pronounced decrease in LAE's antimicrobial efficacy, with the minimal inhibitory and lethal concentrations (MIC and MLC) both increasing with increasing polysaccharide concentration.
24655156	9	58	theme	important	1531:1539	arg1	agent					1601:1605	an antimicrobial agent	1584:1605	an antimicrobial agent in beverage and food products containing anionic polysaccharides	1584:1670	SIGNIFICANCE AND IMPACT OF THE STUDY Results have important implications for the utilization of LAE as an antimicrobial agent in beverage and food products containing anionic polysaccharides.
24655156	9	58	theme	important	1531:1539	arg1	implications					1541:1552	important implications	1531:1552	important implications for the utilization of LAE	1531:1579	SIGNIFICANCE AND IMPACT OF THE STUDY Results have important implications for the utilization of LAE as an antimicrobial agent in beverage and food products containing anionic polysaccharides.
24655156	3	59	theme	antimicrobial	710:722	arg1	efficacy					724:731	LAE's antimicrobial efficacy	704:731	LAE's antimicrobial efficacy	704:731	Combining LAE with either xanthan or λ-carrageenan caused a pronounced decrease in LAE's antimicrobial efficacy, with the minimal inhibitory and lethal concentrations (MIC and MLC) both increasing with increasing polysaccharide concentration.
24655156	3	60	theme	pronounced	681:690	arg1	decrease					692:699	a pronounced decrease	679:699	a pronounced decrease in LAE's antimicrobial efficacy	679:731	Combining LAE with either xanthan or λ-carrageenan caused a pronounced decrease in LAE's antimicrobial efficacy, with the minimal inhibitory and lethal concentrations (MIC and MLC) both increasing with increasing polysaccharide concentration.
24655156	4	61	from	reduction	869:877	arg1	efficacy					896:903	antimicrobial efficacy	882:903	antimicrobial efficacy	882:903	This reduction in antimicrobial efficacy was more pronounced for the addition of λ-carrageenan.
24655156	5	62	theme	activity	994:1001	arg1	loss					986:989	loss	986:989	loss of activity	986:1001	To determine the cause of loss of activity, physical properties of solutions were examined.
24655156	2	63	theme	anionic	538:544	arg1	polysaccharides					546:560	anionic polysaccharides	538:560	anionic polysaccharides	538:560	METHODS AND RESULTS The antimicrobial efficacy of LAE against Saccharomyces cerevisiae, Candida albicans and Zygosaccharomyces bailii in the absence and presence of anionic polysaccharides was assessed by microtitre and macrobroth dilution assays.
24655156	1	64	dep	applications	220:231	arg1	λ-carrageenan					246:258	λ-carrageenan	246:258	λ-carrageenan	246:258	AIMS To investigate the effect of anionic polysaccharides often used in beverage applications (xanthan and λ-carrageenan) on the antimicrobial efficacy of the cationic surfactant lauric arginate (LAE) against typical spoilage yeasts.
24655156	1	64	dep	applications	220:231	arg1	xanthan					234:240	xanthan	234:240	xanthan	234:240	AIMS To investigate the effect of anionic polysaccharides often used in beverage applications (xanthan and λ-carrageenan) on the antimicrobial efficacy of the cationic surfactant lauric arginate (LAE) against typical spoilage yeasts.
24655156	1	64	dep	applications	220:231	arg1	applications					220:231	beverage applications	211:231	beverage applications (xanthan and λ-carrageenan)	211:259	AIMS To investigate the effect of anionic polysaccharides often used in beverage applications (xanthan and λ-carrageenan) on the antimicrobial efficacy of the cationic surfactant lauric arginate (LAE) against typical spoilage yeasts.
24655156	9	65	theme	beverage	1610:1617	arg1	products					1628:1635	beverage and food products	1610:1635	products	1628:1635	SIGNIFICANCE AND IMPACT OF THE STUDY Results have important implications for the utilization of LAE as an antimicrobial agent in beverage and food products containing anionic polysaccharides.
24655156	8	66	theme	antimicrobial	1457:1469	arg1	activity					1471:1478	antimicrobial activity	1457:1478	antimicrobial activity	1457:1478	CONCLUSION Results suggest that electrostatic interactions between LAE and anionic polysaccharides play a major role in complex formation and loss of antimicrobial activity.
24655156	1	67	theme	typical	348:354	arg1	yeasts					365:370	typical spoilage yeasts	348:370	typical spoilage yeasts	348:370	AIMS To investigate the effect of anionic polysaccharides often used in beverage applications (xanthan and λ-carrageenan) on the antimicrobial efficacy of the cationic surfactant lauric arginate (LAE) against typical spoilage yeasts.
24655156	7	68	theme	solution	1285:1292	arg1	composition					1294:1304	solution composition	1285:1304	solution composition	1285:1304	Electrophoresis measurements showed that complexes had varying electrical charges and dimensions depending on solution composition.
24655156	7	69	contain	had	1226:1228	arg2	charges					1249:1255	varying electrical charges	1230:1255	varying electrical charges	1230:1255	Electrophoresis measurements showed that complexes had varying electrical charges and dimensions depending on solution composition.
24655156	7	69	contain	had	1226:1228	arg1	complexes					1216:1224	complexes	1216:1224	complexes	1216:1224	Electrophoresis measurements showed that complexes had varying electrical charges and dimensions depending on solution composition.
24655156	7	69	contain	had	1226:1228	arg2	dimensions					1261:1270	dimensions	1261:1270	dimensions	1261:1270	Electrophoresis measurements showed that complexes had varying electrical charges and dimensions depending on solution composition.
24655156	3	70	dep	concentrations	773:786	arg1	concentrations					773:786	the minimal inhibitory and lethal concentrations	739:786	the minimal inhibitory and lethal concentrations (MIC and MLC)	739:800	Combining LAE with either xanthan or λ-carrageenan caused a pronounced decrease in LAE's antimicrobial efficacy, with the minimal inhibitory and lethal concentrations (MIC and MLC) both increasing with increasing polysaccharide concentration.
24655156	3	70	dep	concentrations	773:786	arg1	both					802:805	both	802:805	both	802:805	Combining LAE with either xanthan or λ-carrageenan caused a pronounced decrease in LAE's antimicrobial efficacy, with the minimal inhibitory and lethal concentrations (MIC and MLC) both increasing with increasing polysaccharide concentration.
24655156	3	70	dep	concentrations	773:786	arg1	MLC					797:799	MLC	797:799	MLC	797:799	Combining LAE with either xanthan or λ-carrageenan caused a pronounced decrease in LAE's antimicrobial efficacy, with the minimal inhibitory and lethal concentrations (MIC and MLC) both increasing with increasing polysaccharide concentration.
24655156	3	70	dep	concentrations	773:786	arg1	MIC					789:791	MIC	789:791	MIC	789:791	Combining LAE with either xanthan or λ-carrageenan caused a pronounced decrease in LAE's antimicrobial efficacy, with the minimal inhibitory and lethal concentrations (MIC and MLC) both increasing with increasing polysaccharide concentration.
25603144	0	0	theme	officinalis	93:103	arg1	L.					105:106	Althaea officinalis L.	85:106	Althaea officinalis L.	85:106	The extraction process optimization of antioxidant polysaccharides from Marshmallow (Althaea officinalis L.) roots.
25603144	1	1	theme	Althaea	445:451	arg1	roots					465:469	Althaea officinalis roots	445:469	Althaea officinalis roots	445:469	Response surface methodology (RSM) with a central composite rotatable design (CCRD) based on five levels was employed to model and optimize four experimental operating conditions of extraction temperature (10-90 °C) and time (6-30 h), particle size (6-24 mm) and water to solid (W/S, 10-50) ratio, obtaining polysaccharides from Althaea officinalis roots with high yield and antioxidant activity.
25603144	4	2	theme	40.0	1155:1158	arg1	°C					1088:1089	extraction temperature 60.90 °C	1059:1089	extraction temperature 60.90 °C	1059:1089	The optimum conditions to maximize yield (10.80%) and antioxidant activity (84.09%) for polysaccharides extraction from A. officinalis roots were extraction temperature 60.90 °C, extraction time 12.01 h, particle size 12.0mm and W/S ratio of 40.0.
25603144	4	2	theme	40.0	1155:1158	arg1	size					1126:1129	particle size 12.0mm	1117:1136	particle size 12.0mm	1117:1136	The optimum conditions to maximize yield (10.80%) and antioxidant activity (84.09%) for polysaccharides extraction from A. officinalis roots were extraction temperature 60.90 °C, extraction time 12.01 h, particle size 12.0mm and W/S ratio of 40.0.
25603144	4	2	theme	40.0	1155:1158	arg1	conditions					925:934	The optimum conditions	913:934	The optimum conditions to maximize yield (10.80%) and antioxidant activity (84.09%) for polysaccharides extraction from A. officinalis roots	913:1052	The optimum conditions to maximize yield (10.80%) and antioxidant activity (84.09%) for polysaccharides extraction from A. officinalis roots were extraction temperature 60.90 °C, extraction time 12.01 h, particle size 12.0mm and W/S ratio of 40.0.
25603144	4	2	theme	40.0	1155:1158	arg1	ratio					1146:1150	W/S ratio	1142:1150	W/S ratio	1142:1150	The optimum conditions to maximize yield (10.80%) and antioxidant activity (84.09%) for polysaccharides extraction from A. officinalis roots were extraction temperature 60.90 °C, extraction time 12.01 h, particle size 12.0mm and W/S ratio of 40.0.
25603144	4	2	theme	40.0	1155:1158	arg1	h					1114:1114	extraction time 12.01 h	1092:1114	extraction time 12.01 h	1092:1114	The optimum conditions to maximize yield (10.80%) and antioxidant activity (84.09%) for polysaccharides extraction from A. officinalis roots were extraction temperature 60.90 °C, extraction time 12.01 h, particle size 12.0mm and W/S ratio of 40.0.
25603144	3	3	theme	extracted	777:785	arg1	polysaccharides					787:801	extracted polysaccharides	777:801	extracted polysaccharides	777:801	Results showed that the most significant (P < 0.05) extraction conditions that affect the yield and antioxidant activity of extracted polysaccharides were the main effect of extraction temperature and the interaction effect of the particle size and W/S ratio.
25603144	0	4	theme	Althaea	85:91	arg1	L.					105:106	Althaea officinalis L.	85:106	Althaea officinalis L.	85:106	The extraction process optimization of antioxidant polysaccharides from Marshmallow (Althaea officinalis L.) roots.
25603144	2	5	theme	R	574:574	arg1	0.966					589:593	> 0.966	587:593	> 0.966	587:593	For each response, a second-order polynomial model with high R(2) values (> 0.966) was developed using multiple linear regression analysis.
25603144	2	5	theme	R	574:574	arg1	values					579:584	high R(2) values	569:584	high R(2) values (> 0.966)	569:594	For each response, a second-order polynomial model with high R(2) values (> 0.966) was developed using multiple linear regression analysis.
25603144	3	6	theme	extraction	705:714	arg1	conditions					716:725	the most significant (P < 0.05) extraction conditions	673:725	the most significant (P < 0.05) extraction conditions that affect the yield and antioxidant activity of extracted polysaccharides	673:801	Results showed that the most significant (P < 0.05) extraction conditions that affect the yield and antioxidant activity of extracted polysaccharides were the main effect of extraction temperature and the interaction effect of the particle size and W/S ratio.
25603144	3	6	theme	extraction	705:714	arg1	effect					817:822	the main effect	808:822	the main effect of extraction temperature	808:848	Results showed that the most significant (P < 0.05) extraction conditions that affect the yield and antioxidant activity of extracted polysaccharides were the main effect of extraction temperature and the interaction effect of the particle size and W/S ratio.
25603144	5	7	theme	models	1252:1257	arg1	suitability					1259:1269	the models suitability	1248:1269	the models suitability for optimizing the polysaccharides extraction conditions	1248:1326	The experimental values were found to be in agreement with those predicted, indicating the models suitability for optimizing the polysaccharides extraction conditions.
25603144	2	8	theme	high	569:572	arg1	0.966					589:593	> 0.966	587:593	> 0.966	587:593	For each response, a second-order polynomial model with high R(2) values (> 0.966) was developed using multiple linear regression analysis.
25603144	2	8	theme	high	569:572	arg1	values					579:584	high R(2) values	569:584	high R(2) values (> 0.966)	569:594	For each response, a second-order polynomial model with high R(2) values (> 0.966) was developed using multiple linear regression analysis.
25603144	5	9	theme	experimental	1165:1176	arg1	values					1178:1183	The experimental values	1161:1183	The experimental values	1161:1183	The experimental values were found to be in agreement with those predicted, indicating the models suitability for optimizing the polysaccharides extraction conditions.
25603144	3	10	theme	particle	884:891	arg1	size					893:896	the particle size	880:896	the particle size	880:896	Results showed that the most significant (P < 0.05) extraction conditions that affect the yield and antioxidant activity of extracted polysaccharides were the main effect of extraction temperature and the interaction effect of the particle size and W/S ratio.
25603144	1	11	theme	officinalis	453:463	arg1	roots					465:469	Althaea officinalis roots	445:469	Althaea officinalis roots	445:469	Response surface methodology (RSM) with a central composite rotatable design (CCRD) based on five levels was employed to model and optimize four experimental operating conditions of extraction temperature (10-90 °C) and time (6-30 h), particle size (6-24 mm) and water to solid (W/S, 10-50) ratio, obtaining polysaccharides from Althaea officinalis roots with high yield and antioxidant activity.
25603144	2	12	theme	linear	625:630	arg1	analysis					643:650	multiple linear regression analysis	616:650	multiple linear regression analysis	616:650	For each response, a second-order polynomial model with high R(2) values (> 0.966) was developed using multiple linear regression analysis.
25603144	3	13	theme	polysaccharides	787:801	arg1	yield					743:747	the yield	739:747	the yield	739:747	Results showed that the most significant (P < 0.05) extraction conditions that affect the yield and antioxidant activity of extracted polysaccharides were the main effect of extraction temperature and the interaction effect of the particle size and W/S ratio.
25603144	3	13	theme	polysaccharides	787:801	arg1	activity					765:772	antioxidant activity	753:772	antioxidant activity	753:772	Results showed that the most significant (P < 0.05) extraction conditions that affect the yield and antioxidant activity of extracted polysaccharides were the main effect of extraction temperature and the interaction effect of the particle size and W/S ratio.
25603144	1	14	with	methodology	133:143	arg1	CCRD					194:197	CCRD	194:197	CCRD	194:197	Response surface methodology (RSM) with a central composite rotatable design (CCRD) based on five levels was employed to model and optimize four experimental operating conditions of extraction temperature (10-90 °C) and time (6-30 h), particle size (6-24 mm) and water to solid (W/S, 10-50) ratio, obtaining polysaccharides from Althaea officinalis roots with high yield and antioxidant activity.
25603144	1	14	with	methodology	133:143	arg1	design					186:191	a central composite rotatable design	156:191	a central composite rotatable design (CCRD)	156:198	Response surface methodology (RSM) with a central composite rotatable design (CCRD) based on five levels was employed to model and optimize four experimental operating conditions of extraction temperature (10-90 °C) and time (6-30 h), particle size (6-24 mm) and water to solid (W/S, 10-50) ratio, obtaining polysaccharides from Althaea officinalis roots with high yield and antioxidant activity.
25603144	3	15	theme	size	893:896	arg1	effect					870:875	the interaction effect	854:875	the interaction effect of the particle size and W/S ratio	854:910	Results showed that the most significant (P < 0.05) extraction conditions that affect the yield and antioxidant activity of extracted polysaccharides were the main effect of extraction temperature and the interaction effect of the particle size and W/S ratio.
25603144	3	15	theme	size	893:896	arg1	conditions					716:725	the most significant (P < 0.05) extraction conditions	673:725	the most significant (P < 0.05) extraction conditions that affect the yield and antioxidant activity of extracted polysaccharides	673:801	Results showed that the most significant (P < 0.05) extraction conditions that affect the yield and antioxidant activity of extracted polysaccharides were the main effect of extraction temperature and the interaction effect of the particle size and W/S ratio.
25603144	3	15	theme	size	893:896	arg1	effect					817:822	the main effect	808:822	the main effect of extraction temperature	808:848	Results showed that the most significant (P < 0.05) extraction conditions that affect the yield and antioxidant activity of extracted polysaccharides were the main effect of extraction temperature and the interaction effect of the particle size and W/S ratio.
25603144	1	16	theme	central	158:164	arg1	CCRD					194:197	CCRD	194:197	CCRD	194:197	Response surface methodology (RSM) with a central composite rotatable design (CCRD) based on five levels was employed to model and optimize four experimental operating conditions of extraction temperature (10-90 °C) and time (6-30 h), particle size (6-24 mm) and water to solid (W/S, 10-50) ratio, obtaining polysaccharides from Althaea officinalis roots with high yield and antioxidant activity.
25603144	1	16	theme	central	158:164	arg1	design					186:191	a central composite rotatable design	156:191	a central composite rotatable design (CCRD)	156:198	Response surface methodology (RSM) with a central composite rotatable design (CCRD) based on five levels was employed to model and optimize four experimental operating conditions of extraction temperature (10-90 °C) and time (6-30 h), particle size (6-24 mm) and water to solid (W/S, 10-50) ratio, obtaining polysaccharides from Althaea officinalis roots with high yield and antioxidant activity.
25603144	2	17	theme	second-order	534:545	arg1	model					558:562	a second-order polynomial model	532:562	a second-order polynomial model with high R(2) values (> 0.966)	532:594	For each response, a second-order polynomial model with high R(2) values (> 0.966) was developed using multiple linear regression analysis.
25603144	1	18	theme	solid	388:392	arg1	ratio					407:411	solid (W/S, 10-50) ratio	388:411	ratio	407:411	Response surface methodology (RSM) with a central composite rotatable design (CCRD) based on five levels was employed to model and optimize four experimental operating conditions of extraction temperature (10-90 °C) and time (6-30 h), particle size (6-24 mm) and water to solid (W/S, 10-50) ratio, obtaining polysaccharides from Althaea officinalis roots with high yield and antioxidant activity.
25603144	2	19	theme	regression	632:641	arg1	analysis					643:650	multiple linear regression analysis	616:650	multiple linear regression analysis	616:650	For each response, a second-order polynomial model with high R(2) values (> 0.966) was developed using multiple linear regression analysis.
25603144	1	20	theme	composite	166:174	arg1	CCRD					194:197	CCRD	194:197	CCRD	194:197	Response surface methodology (RSM) with a central composite rotatable design (CCRD) based on five levels was employed to model and optimize four experimental operating conditions of extraction temperature (10-90 °C) and time (6-30 h), particle size (6-24 mm) and water to solid (W/S, 10-50) ratio, obtaining polysaccharides from Althaea officinalis roots with high yield and antioxidant activity.
25603144	1	20	theme	composite	166:174	arg1	design					186:191	a central composite rotatable design	156:191	a central composite rotatable design (CCRD)	156:198	Response surface methodology (RSM) with a central composite rotatable design (CCRD) based on five levels was employed to model and optimize four experimental operating conditions of extraction temperature (10-90 °C) and time (6-30 h), particle size (6-24 mm) and water to solid (W/S, 10-50) ratio, obtaining polysaccharides from Althaea officinalis roots with high yield and antioxidant activity.
25603144	4	21	theme	A.	1033:1034	arg1	roots					1048:1052	A. officinalis roots	1033:1052	A. officinalis roots	1033:1052	The optimum conditions to maximize yield (10.80%) and antioxidant activity (84.09%) for polysaccharides extraction from A. officinalis roots were extraction temperature 60.90 °C, extraction time 12.01 h, particle size 12.0mm and W/S ratio of 40.0.
25603144	4	22	theme	temperature	1070:1080	arg1	°C					1088:1089	extraction temperature 60.90 °C	1059:1089	extraction temperature 60.90 °C	1059:1089	The optimum conditions to maximize yield (10.80%) and antioxidant activity (84.09%) for polysaccharides extraction from A. officinalis roots were extraction temperature 60.90 °C, extraction time 12.01 h, particle size 12.0mm and W/S ratio of 40.0.
25603144	4	22	theme	temperature	1070:1080	arg1	conditions					925:934	The optimum conditions	913:934	The optimum conditions to maximize yield (10.80%) and antioxidant activity (84.09%) for polysaccharides extraction from A. officinalis roots	913:1052	The optimum conditions to maximize yield (10.80%) and antioxidant activity (84.09%) for polysaccharides extraction from A. officinalis roots were extraction temperature 60.90 °C, extraction time 12.01 h, particle size 12.0mm and W/S ratio of 40.0.
25603144	1	23	theme	high	476:479	arg1	yield					481:485	high yield	476:485	high yield	476:485	Response surface methodology (RSM) with a central composite rotatable design (CCRD) based on five levels was employed to model and optimize four experimental operating conditions of extraction temperature (10-90 °C) and time (6-30 h), particle size (6-24 mm) and water to solid (W/S, 10-50) ratio, obtaining polysaccharides from Althaea officinalis roots with high yield and antioxidant activity.
25603144	1	24	theme	rotatable	176:184	arg1	CCRD					194:197	CCRD	194:197	CCRD	194:197	Response surface methodology (RSM) with a central composite rotatable design (CCRD) based on five levels was employed to model and optimize four experimental operating conditions of extraction temperature (10-90 °C) and time (6-30 h), particle size (6-24 mm) and water to solid (W/S, 10-50) ratio, obtaining polysaccharides from Althaea officinalis roots with high yield and antioxidant activity.
25603144	1	24	theme	rotatable	176:184	arg1	design					186:191	a central composite rotatable design	156:191	a central composite rotatable design (CCRD)	156:198	Response surface methodology (RSM) with a central composite rotatable design (CCRD) based on five levels was employed to model and optimize four experimental operating conditions of extraction temperature (10-90 °C) and time (6-30 h), particle size (6-24 mm) and water to solid (W/S, 10-50) ratio, obtaining polysaccharides from Althaea officinalis roots with high yield and antioxidant activity.
25603144	2	25	with	model	558:562	arg1	0.966					589:593	> 0.966	587:593	> 0.966	587:593	For each response, a second-order polynomial model with high R(2) values (> 0.966) was developed using multiple linear regression analysis.
25603144	2	25	with	model	558:562	arg1	values					579:584	high R(2) values	569:584	high R(2) values (> 0.966)	569:594	For each response, a second-order polynomial model with high R(2) values (> 0.966) was developed using multiple linear regression analysis.
25603144	1	26	theme	W/S	395:397	arg1	ratio					407:411	solid (W/S, 10-50) ratio	388:411	ratio	407:411	Response surface methodology (RSM) with a central composite rotatable design (CCRD) based on five levels was employed to model and optimize four experimental operating conditions of extraction temperature (10-90 °C) and time (6-30 h), particle size (6-24 mm) and water to solid (W/S, 10-50) ratio, obtaining polysaccharides from Althaea officinalis roots with high yield and antioxidant activity.
25603144	2	27	theme	polynomial	547:556	arg1	model					558:562	a second-order polynomial model	532:562	a second-order polynomial model with high R(2) values (> 0.966)	532:594	For each response, a second-order polynomial model with high R(2) values (> 0.966) was developed using multiple linear regression analysis.
25603144	4	28	theme	antioxidant	967:977	arg1	activity					979:986	antioxidant activity	967:986	antioxidant activity (84.09%)	967:995	The optimum conditions to maximize yield (10.80%) and antioxidant activity (84.09%) for polysaccharides extraction from A. officinalis roots were extraction temperature 60.90 °C, extraction time 12.01 h, particle size 12.0mm and W/S ratio of 40.0.
25603144	4	28	theme	antioxidant	967:977	arg1	%					994:994	84.09%	989:994	84.09%	989:994	The optimum conditions to maximize yield (10.80%) and antioxidant activity (84.09%) for polysaccharides extraction from A. officinalis roots were extraction temperature 60.90 °C, extraction time 12.01 h, particle size 12.0mm and W/S ratio of 40.0.
25603144	4	29	theme	optimum	917:923	arg1	°C					1088:1089	extraction temperature 60.90 °C	1059:1089	extraction temperature 60.90 °C	1059:1089	The optimum conditions to maximize yield (10.80%) and antioxidant activity (84.09%) for polysaccharides extraction from A. officinalis roots were extraction temperature 60.90 °C, extraction time 12.01 h, particle size 12.0mm and W/S ratio of 40.0.
25603144	4	29	theme	optimum	917:923	arg1	conditions					925:934	The optimum conditions	913:934	The optimum conditions to maximize yield (10.80%) and antioxidant activity (84.09%) for polysaccharides extraction from A. officinalis roots	913:1052	The optimum conditions to maximize yield (10.80%) and antioxidant activity (84.09%) for polysaccharides extraction from A. officinalis roots were extraction temperature 60.90 °C, extraction time 12.01 h, particle size 12.0mm and W/S ratio of 40.0.
25603144	3	30	theme	ratio	906:910	arg1	effect					870:875	the interaction effect	854:875	the interaction effect of the particle size and W/S ratio	854:910	Results showed that the most significant (P < 0.05) extraction conditions that affect the yield and antioxidant activity of extracted polysaccharides were the main effect of extraction temperature and the interaction effect of the particle size and W/S ratio.
25603144	3	30	theme	ratio	906:910	arg1	conditions					716:725	the most significant (P < 0.05) extraction conditions	673:725	the most significant (P < 0.05) extraction conditions that affect the yield and antioxidant activity of extracted polysaccharides	673:801	Results showed that the most significant (P < 0.05) extraction conditions that affect the yield and antioxidant activity of extracted polysaccharides were the main effect of extraction temperature and the interaction effect of the particle size and W/S ratio.
25603144	3	30	theme	ratio	906:910	arg1	effect					817:822	the main effect	808:822	the main effect of extraction temperature	808:848	Results showed that the most significant (P < 0.05) extraction conditions that affect the yield and antioxidant activity of extracted polysaccharides were the main effect of extraction temperature and the interaction effect of the particle size and W/S ratio.
25603144	4	31	dep	conditions	925:934	arg1	maximize					939:946	maximize	939:946	to maximize yield (10.80%) and antioxidant activity (84.09%) for polysaccharides extraction from A. officinalis roots	936:1052	The optimum conditions to maximize yield (10.80%) and antioxidant activity (84.09%) for polysaccharides extraction from A. officinalis roots were extraction temperature 60.90 °C, extraction time 12.01 h, particle size 12.0mm and W/S ratio of 40.0.
25603144	5	32	theme	polysaccharides	1290:1304	arg1	conditions					1317:1326	the polysaccharides extraction conditions	1286:1326	the polysaccharides extraction conditions	1286:1326	The experimental values were found to be in agreement with those predicted, indicating the models suitability for optimizing the polysaccharides extraction conditions.
25603144	4	33	theme	time	1103:1106	arg1	h					1114:1114	extraction time 12.01 h	1092:1114	extraction time 12.01 h	1092:1114	The optimum conditions to maximize yield (10.80%) and antioxidant activity (84.09%) for polysaccharides extraction from A. officinalis roots were extraction temperature 60.90 °C, extraction time 12.01 h, particle size 12.0mm and W/S ratio of 40.0.
25603144	1	34	theme	experimental	261:272	arg1	conditions					284:293	four experimental operating conditions	256:293	four experimental operating conditions of extraction temperature (10-90 °C)	256:330	Response surface methodology (RSM) with a central composite rotatable design (CCRD) based on five levels was employed to model and optimize four experimental operating conditions of extraction temperature (10-90 °C) and time (6-30 h), particle size (6-24 mm) and water to solid (W/S, 10-50) ratio, obtaining polysaccharides from Althaea officinalis roots with high yield and antioxidant activity.
25603144	3	35	theme	extraction	827:836	arg1	temperature					838:848	extraction temperature	827:848	extraction temperature	827:848	Results showed that the most significant (P < 0.05) extraction conditions that affect the yield and antioxidant activity of extracted polysaccharides were the main effect of extraction temperature and the interaction effect of the particle size and W/S ratio.
25603144	4	36	theme	W/S	1142:1144	arg1	ratio					1146:1150	W/S ratio	1142:1150	W/S ratio	1142:1150	The optimum conditions to maximize yield (10.80%) and antioxidant activity (84.09%) for polysaccharides extraction from A. officinalis roots were extraction temperature 60.90 °C, extraction time 12.01 h, particle size 12.0mm and W/S ratio of 40.0.
25603144	0	37	dep	Marshmallow	72:82	arg1	L.					105:106	Althaea officinalis L.	85:106	Althaea officinalis L.	85:106	The extraction process optimization of antioxidant polysaccharides from Marshmallow (Althaea officinalis L.) roots.
25603144	1	38	theme	antioxidant	491:501	arg1	activity					503:510	antioxidant activity	491:510	antioxidant activity	491:510	Response surface methodology (RSM) with a central composite rotatable design (CCRD) based on five levels was employed to model and optimize four experimental operating conditions of extraction temperature (10-90 °C) and time (6-30 h), particle size (6-24 mm) and water to solid (W/S, 10-50) ratio, obtaining polysaccharides from Althaea officinalis roots with high yield and antioxidant activity.
25603144	0	39	theme	antioxidant	39:49	arg1	polysaccharides					51:65	antioxidant polysaccharides	39:65	antioxidant polysaccharides from Marshmallow (Althaea officinalis L.) roots	39:113	The extraction process optimization of antioxidant polysaccharides from Marshmallow (Althaea officinalis L.) roots.
25603144	1	40	theme	operating	274:282	arg1	conditions					284:293	four experimental operating conditions	256:293	four experimental operating conditions of extraction temperature (10-90 °C)	256:330	Response surface methodology (RSM) with a central composite rotatable design (CCRD) based on five levels was employed to model and optimize four experimental operating conditions of extraction temperature (10-90 °C) and time (6-30 h), particle size (6-24 mm) and water to solid (W/S, 10-50) ratio, obtaining polysaccharides from Althaea officinalis roots with high yield and antioxidant activity.
25603144	3	41	theme	main	812:815	arg1	conditions					716:725	the most significant (P < 0.05) extraction conditions	673:725	the most significant (P < 0.05) extraction conditions that affect the yield and antioxidant activity of extracted polysaccharides	673:801	Results showed that the most significant (P < 0.05) extraction conditions that affect the yield and antioxidant activity of extracted polysaccharides were the main effect of extraction temperature and the interaction effect of the particle size and W/S ratio.
25603144	3	41	theme	main	812:815	arg1	effect					817:822	the main effect	808:822	the main effect of extraction temperature	808:848	Results showed that the most significant (P < 0.05) extraction conditions that affect the yield and antioxidant activity of extracted polysaccharides were the main effect of extraction temperature and the interaction effect of the particle size and W/S ratio.
25603144	5	42	theme	extraction	1306:1315	arg1	conditions					1317:1326	the polysaccharides extraction conditions	1286:1326	the polysaccharides extraction conditions	1286:1326	The experimental values were found to be in agreement with those predicted, indicating the models suitability for optimizing the polysaccharides extraction conditions.
25603144	1	43	theme	particle	351:358	arg1	size					360:363	particle size	351:363	particle size (6-24 mm)	351:373	Response surface methodology (RSM) with a central composite rotatable design (CCRD) based on five levels was employed to model and optimize four experimental operating conditions of extraction temperature (10-90 °C) and time (6-30 h), particle size (6-24 mm) and water to solid (W/S, 10-50) ratio, obtaining polysaccharides from Althaea officinalis roots with high yield and antioxidant activity.
25603144	1	43	theme	particle	351:358	arg1	mm					371:372	6-24 mm	366:372	6-24 mm	366:372	Response surface methodology (RSM) with a central composite rotatable design (CCRD) based on five levels was employed to model and optimize four experimental operating conditions of extraction temperature (10-90 °C) and time (6-30 h), particle size (6-24 mm) and water to solid (W/S, 10-50) ratio, obtaining polysaccharides from Althaea officinalis roots with high yield and antioxidant activity.
25603144	4	44	from	roots	1048:1052	arg1	extraction					1017:1026	polysaccharides extraction	1001:1026	polysaccharides extraction from A. officinalis roots	1001:1052	The optimum conditions to maximize yield (10.80%) and antioxidant activity (84.09%) for polysaccharides extraction from A. officinalis roots were extraction temperature 60.90 °C, extraction time 12.01 h, particle size 12.0mm and W/S ratio of 40.0.
25603144	4	45	theme	officinalis	1036:1046	arg1	roots					1048:1052	A. officinalis roots	1033:1052	A. officinalis roots	1033:1052	The optimum conditions to maximize yield (10.80%) and antioxidant activity (84.09%) for polysaccharides extraction from A. officinalis roots were extraction temperature 60.90 °C, extraction time 12.01 h, particle size 12.0mm and W/S ratio of 40.0.
25603144	1	46	theme	Response	116:123	arg1	methodology					133:143	Response surface methodology	116:143	Response surface methodology (RSM) with a central composite rotatable design (CCRD) based on five levels	116:219	Response surface methodology (RSM) with a central composite rotatable design (CCRD) based on five levels was employed to model and optimize four experimental operating conditions of extraction temperature (10-90 °C) and time (6-30 h), particle size (6-24 mm) and water to solid (W/S, 10-50) ratio, obtaining polysaccharides from Althaea officinalis roots with high yield and antioxidant activity.
25603144	1	46	theme	Response	116:123	arg1	RSM					146:148	RSM	146:148	RSM	146:148	Response surface methodology (RSM) with a central composite rotatable design (CCRD) based on five levels was employed to model and optimize four experimental operating conditions of extraction temperature (10-90 °C) and time (6-30 h), particle size (6-24 mm) and water to solid (W/S, 10-50) ratio, obtaining polysaccharides from Althaea officinalis roots with high yield and antioxidant activity.
25603144	0	47	theme	polysaccharides	51:65	arg1	optimization					23:34	optimization	23:34	optimization of antioxidant polysaccharides from Marshmallow (Althaea officinalis L.) roots	23:113	The extraction process optimization of antioxidant polysaccharides from Marshmallow (Althaea officinalis L.) roots.
25603144	1	48	theme	extraction	298:307	arg1	°C					328:329	10-90 °C	322:329	10-90 °C	322:329	Response surface methodology (RSM) with a central composite rotatable design (CCRD) based on five levels was employed to model and optimize four experimental operating conditions of extraction temperature (10-90 °C) and time (6-30 h), particle size (6-24 mm) and water to solid (W/S, 10-50) ratio, obtaining polysaccharides from Althaea officinalis roots with high yield and antioxidant activity.
25603144	1	48	theme	extraction	298:307	arg1	temperature					309:319	extraction temperature	298:319	extraction temperature (10-90 °C)	298:330	Response surface methodology (RSM) with a central composite rotatable design (CCRD) based on five levels was employed to model and optimize four experimental operating conditions of extraction temperature (10-90 °C) and time (6-30 h), particle size (6-24 mm) and water to solid (W/S, 10-50) ratio, obtaining polysaccharides from Althaea officinalis roots with high yield and antioxidant activity.
25603144	4	49	theme	particle	1117:1124	arg1	size					1126:1129	particle size 12.0mm	1117:1136	particle size 12.0mm	1117:1136	The optimum conditions to maximize yield (10.80%) and antioxidant activity (84.09%) for polysaccharides extraction from A. officinalis roots were extraction temperature 60.90 °C, extraction time 12.01 h, particle size 12.0mm and W/S ratio of 40.0.
25603144	2	50	theme	>	587:587	arg1	0.966					589:593	> 0.966	587:593	> 0.966	587:593	For each response, a second-order polynomial model with high R(2) values (> 0.966) was developed using multiple linear regression analysis.
25603144	2	50	theme	>	587:587	arg1	values					579:584	high R(2) values	569:584	high R(2) values (> 0.966)	569:594	For each response, a second-order polynomial model with high R(2) values (> 0.966) was developed using multiple linear regression analysis.
25603144	1	51	theme	surface	125:131	arg1	methodology					133:143	Response surface methodology	116:143	Response surface methodology (RSM) with a central composite rotatable design (CCRD) based on five levels	116:219	Response surface methodology (RSM) with a central composite rotatable design (CCRD) based on five levels was employed to model and optimize four experimental operating conditions of extraction temperature (10-90 °C) and time (6-30 h), particle size (6-24 mm) and water to solid (W/S, 10-50) ratio, obtaining polysaccharides from Althaea officinalis roots with high yield and antioxidant activity.
25603144	1	51	theme	surface	125:131	arg1	RSM					146:148	RSM	146:148	RSM	146:148	Response surface methodology (RSM) with a central composite rotatable design (CCRD) based on five levels was employed to model and optimize four experimental operating conditions of extraction temperature (10-90 °C) and time (6-30 h), particle size (6-24 mm) and water to solid (W/S, 10-50) ratio, obtaining polysaccharides from Althaea officinalis roots with high yield and antioxidant activity.
25603144	1	52	theme	temperature	309:319	arg1	water					379:383	water	379:383	water	379:383	Response surface methodology (RSM) with a central composite rotatable design (CCRD) based on five levels was employed to model and optimize four experimental operating conditions of extraction temperature (10-90 °C) and time (6-30 h), particle size (6-24 mm) and water to solid (W/S, 10-50) ratio, obtaining polysaccharides from Althaea officinalis roots with high yield and antioxidant activity.
25603144	1	52	theme	temperature	309:319	arg1	time					336:339	time	336:339	time (6-30 h)	336:348	Response surface methodology (RSM) with a central composite rotatable design (CCRD) based on five levels was employed to model and optimize four experimental operating conditions of extraction temperature (10-90 °C) and time (6-30 h), particle size (6-24 mm) and water to solid (W/S, 10-50) ratio, obtaining polysaccharides from Althaea officinalis roots with high yield and antioxidant activity.
25603144	1	52	theme	temperature	309:319	arg1	size					360:363	particle size	351:363	particle size (6-24 mm)	351:373	Response surface methodology (RSM) with a central composite rotatable design (CCRD) based on five levels was employed to model and optimize four experimental operating conditions of extraction temperature (10-90 °C) and time (6-30 h), particle size (6-24 mm) and water to solid (W/S, 10-50) ratio, obtaining polysaccharides from Althaea officinalis roots with high yield and antioxidant activity.
25603144	1	52	theme	temperature	309:319	arg1	mm					371:372	6-24 mm	366:372	6-24 mm	366:372	Response surface methodology (RSM) with a central composite rotatable design (CCRD) based on five levels was employed to model and optimize four experimental operating conditions of extraction temperature (10-90 °C) and time (6-30 h), particle size (6-24 mm) and water to solid (W/S, 10-50) ratio, obtaining polysaccharides from Althaea officinalis roots with high yield and antioxidant activity.
25603144	1	52	theme	temperature	309:319	arg1	h					347:347	6-30 h	342:347	6-30 h	342:347	Response surface methodology (RSM) with a central composite rotatable design (CCRD) based on five levels was employed to model and optimize four experimental operating conditions of extraction temperature (10-90 °C) and time (6-30 h), particle size (6-24 mm) and water to solid (W/S, 10-50) ratio, obtaining polysaccharides from Althaea officinalis roots with high yield and antioxidant activity.
25603144	1	52	theme	temperature	309:319	arg1	conditions					284:293	four experimental operating conditions	256:293	four experimental operating conditions of extraction temperature (10-90 °C)	256:330	Response surface methodology (RSM) with a central composite rotatable design (CCRD) based on five levels was employed to model and optimize four experimental operating conditions of extraction temperature (10-90 °C) and time (6-30 h), particle size (6-24 mm) and water to solid (W/S, 10-50) ratio, obtaining polysaccharides from Althaea officinalis roots with high yield and antioxidant activity.
25603144	0	53	from	roots	109:113	arg1	optimization					23:34	optimization	23:34	optimization of antioxidant polysaccharides from Marshmallow (Althaea officinalis L.) roots	23:113	The extraction process optimization of antioxidant polysaccharides from Marshmallow (Althaea officinalis L.) roots.
25603144	0	53	from	roots	109:113	arg1	polysaccharides					51:65	antioxidant polysaccharides	39:65	antioxidant polysaccharides from Marshmallow (Althaea officinalis L.) roots	39:113	The extraction process optimization of antioxidant polysaccharides from Marshmallow (Althaea officinalis L.) roots.
25603144	3	54	theme	W/S	902:904	arg1	ratio					906:910	W/S ratio	902:910	W/S ratio	902:910	Results showed that the most significant (P < 0.05) extraction conditions that affect the yield and antioxidant activity of extracted polysaccharides were the main effect of extraction temperature and the interaction effect of the particle size and W/S ratio.
25603144	4	55	theme	extraction	1059:1068	arg1	°C					1088:1089	extraction temperature 60.90 °C	1059:1089	extraction temperature 60.90 °C	1059:1089	The optimum conditions to maximize yield (10.80%) and antioxidant activity (84.09%) for polysaccharides extraction from A. officinalis roots were extraction temperature 60.90 °C, extraction time 12.01 h, particle size 12.0mm and W/S ratio of 40.0.
25603144	4	55	theme	extraction	1059:1068	arg1	conditions					925:934	The optimum conditions	913:934	The optimum conditions to maximize yield (10.80%) and antioxidant activity (84.09%) for polysaccharides extraction from A. officinalis roots	913:1052	The optimum conditions to maximize yield (10.80%) and antioxidant activity (84.09%) for polysaccharides extraction from A. officinalis roots were extraction temperature 60.90 °C, extraction time 12.01 h, particle size 12.0mm and W/S ratio of 40.0.
25603144	5	56	with	agreement	1205:1213	arg1	those					1220:1224	those	1220:1224	those	1220:1224	The experimental values were found to be in agreement with those predicted, indicating the models suitability for optimizing the polysaccharides extraction conditions.
25603144	3	57	theme	temperature	838:848	arg1	effect					870:875	the interaction effect	854:875	the interaction effect of the particle size and W/S ratio	854:910	Results showed that the most significant (P < 0.05) extraction conditions that affect the yield and antioxidant activity of extracted polysaccharides were the main effect of extraction temperature and the interaction effect of the particle size and W/S ratio.
25603144	3	57	theme	temperature	838:848	arg1	conditions					716:725	the most significant (P < 0.05) extraction conditions	673:725	the most significant (P < 0.05) extraction conditions that affect the yield and antioxidant activity of extracted polysaccharides	673:801	Results showed that the most significant (P < 0.05) extraction conditions that affect the yield and antioxidant activity of extracted polysaccharides were the main effect of extraction temperature and the interaction effect of the particle size and W/S ratio.
25603144	3	57	theme	temperature	838:848	arg1	effect					817:822	the main effect	808:822	the main effect of extraction temperature	808:848	Results showed that the most significant (P < 0.05) extraction conditions that affect the yield and antioxidant activity of extracted polysaccharides were the main effect of extraction temperature and the interaction effect of the particle size and W/S ratio.
25603144	2	58	theme	multiple	616:623	arg1	analysis					643:650	multiple linear regression analysis	616:650	multiple linear regression analysis	616:650	For each response, a second-order polynomial model with high R(2) values (> 0.966) was developed using multiple linear regression analysis.
25603144	3	59	theme	P	695:695	arg1	significant					682:692	significant	682:692	significant	682:692	Results showed that the most significant (P < 0.05) extraction conditions that affect the yield and antioxidant activity of extracted polysaccharides were the main effect of extraction temperature and the interaction effect of the particle size and W/S ratio.
25603144	3	59	theme	P	695:695	arg1	<					697:697	P < 0.05	695:702	P < 0.05	695:702	Results showed that the most significant (P < 0.05) extraction conditions that affect the yield and antioxidant activity of extracted polysaccharides were the main effect of extraction temperature and the interaction effect of the particle size and W/S ratio.
25603144	4	60	theme	polysaccharides	1001:1015	arg1	extraction					1017:1026	polysaccharides extraction	1001:1026	polysaccharides extraction from A. officinalis roots	1001:1052	The optimum conditions to maximize yield (10.80%) and antioxidant activity (84.09%) for polysaccharides extraction from A. officinalis roots were extraction temperature 60.90 °C, extraction time 12.01 h, particle size 12.0mm and W/S ratio of 40.0.
25603144	4	61	theme	extraction	1092:1101	arg1	h					1114:1114	extraction time 12.01 h	1092:1114	extraction time 12.01 h	1092:1114	The optimum conditions to maximize yield (10.80%) and antioxidant activity (84.09%) for polysaccharides extraction from A. officinalis roots were extraction temperature 60.90 °C, extraction time 12.01 h, particle size 12.0mm and W/S ratio of 40.0.
25603144	0	62	theme	Marshmallow	72:82	arg1	roots					109:113	Marshmallow (Althaea officinalis L.) roots	72:113	Marshmallow (Althaea officinalis L.) roots	72:113	The extraction process optimization of antioxidant polysaccharides from Marshmallow (Althaea officinalis L.) roots.
25603144	0	63	from	optimization	23:34	arg1	roots					109:113	Marshmallow (Althaea officinalis L.) roots	72:113	Marshmallow (Althaea officinalis L.) roots	72:113	The extraction process optimization of antioxidant polysaccharides from Marshmallow (Althaea officinalis L.) roots.
25603144	1	64	from	roots	465:469	arg1	polysaccharides					424:438	polysaccharides	424:438	polysaccharides from Althaea officinalis roots	424:469	Response surface methodology (RSM) with a central composite rotatable design (CCRD) based on five levels was employed to model and optimize four experimental operating conditions of extraction temperature (10-90 °C) and time (6-30 h), particle size (6-24 mm) and water to solid (W/S, 10-50) ratio, obtaining polysaccharides from Althaea officinalis roots with high yield and antioxidant activity.
25603144	3	65	theme	interaction	858:868	arg1	effect					870:875	the interaction effect	854:875	the interaction effect of the particle size and W/S ratio	854:910	Results showed that the most significant (P < 0.05) extraction conditions that affect the yield and antioxidant activity of extracted polysaccharides were the main effect of extraction temperature and the interaction effect of the particle size and W/S ratio.
25603144	3	66	theme	antioxidant	753:763	arg1	activity					765:772	antioxidant activity	753:772	antioxidant activity	753:772	Results showed that the most significant (P < 0.05) extraction conditions that affect the yield and antioxidant activity of extracted polysaccharides were the main effect of extraction temperature and the interaction effect of the particle size and W/S ratio.
25603144	3	67	theme	significant	682:692	arg1	conditions					716:725	the most significant (P < 0.05) extraction conditions	673:725	the most significant (P < 0.05) extraction conditions that affect the yield and antioxidant activity of extracted polysaccharides	673:801	Results showed that the most significant (P < 0.05) extraction conditions that affect the yield and antioxidant activity of extracted polysaccharides were the main effect of extraction temperature and the interaction effect of the particle size and W/S ratio.
25603144	3	67	theme	significant	682:692	arg1	effect					817:822	the main effect	808:822	the main effect of extraction temperature	808:848	Results showed that the most significant (P < 0.05) extraction conditions that affect the yield and antioxidant activity of extracted polysaccharides were the main effect of extraction temperature and the interaction effect of the particle size and W/S ratio.
28347786	5	0	theme	insulin	1064:1070	arg1	receptor					1072:1079	the insulin receptor	1060:1079	the insulin receptor	1060:1079	Histopathological observations suggested that GP-1, GP-3, and GP-4 could alleviate injury in pancreatic islet cells, and Western blot analysis showed that these GPs upregulated gene expression of the insulin receptor, insulin receptor substrate 2, Akt, and glucose transporter type 4.
28347786	4	1	theme	protein	638:644	arg1	levels					586:591	levels	586:591	levels of fasting blood glucose, glucosylated serum protein, serum insulin, homeostasis model assessment of insulin resistance, total cholesterol, triglyceride and serum alanine transaminase	586:775	In addition, GPs efficiently decreased levels of fasting blood glucose, glucosylated serum protein, serum insulin, homeostasis model assessment of insulin resistance, total cholesterol, triglyceride and serum alanine transaminase, improved oral glucose tolerance, and increased insulin sensitivity in rats with T2DM.
28347786	2	2	theme	monosaccharide	325:338	arg1	compositions					340:351	monosaccharide compositions	325:351	monosaccharide compositions	325:351	The chemical characterization and monosaccharide compositions of GPs, named as GP-1, GP-2, GP-3, and GP-4, were determined by PMP-HPLC and FT-IR.
28347786	5	3	theme	glucose	1121:1127	arg1	type					1141:1144	glucose transporter type 4	1121:1146	glucose transporter type 4	1121:1146	Histopathological observations suggested that GP-1, GP-3, and GP-4 could alleviate injury in pancreatic islet cells, and Western blot analysis showed that these GPs upregulated gene expression of the insulin receptor, insulin receptor substrate 2, Akt, and glucose transporter type 4.
28347786	6	4	theme	cirrhosis	1291:1299	arg1	risk					1245:1248	the risk	1241:1248	the risk of hyperlipidemia, vascular disease, and cirrhosis	1241:1299	Taken together, these data suggest that GPs may be beneficial in treating T2DM and reducing the risk of hyperlipidemia, vascular disease, and cirrhosis via the PI3K/Akt signaling pathway.
28347786	5	5	theme	receptor	1072:1079	arg1	expression					1046:1055	gene expression	1041:1055	gene expression of the insulin receptor, insulin receptor substrate 2, Akt, and glucose transporter type 4	1041:1146	Histopathological observations suggested that GP-1, GP-3, and GP-4 could alleviate injury in pancreatic islet cells, and Western blot analysis showed that these GPs upregulated gene expression of the insulin receptor, insulin receptor substrate 2, Akt, and glucose transporter type 4.
28347786	4	6	theme	serum	632:636	arg1	protein					638:644	glucosylated serum protein	619:644	glucosylated serum protein	619:644	In addition, GPs efficiently decreased levels of fasting blood glucose, glucosylated serum protein, serum insulin, homeostasis model assessment of insulin resistance, total cholesterol, triglyceride and serum alanine transaminase, improved oral glucose tolerance, and increased insulin sensitivity in rats with T2DM.
28347786	5	7	theme	transporter	1129:1139	arg1	type					1141:1144	glucose transporter type 4	1121:1146	glucose transporter type 4	1121:1146	Histopathological observations suggested that GP-1, GP-3, and GP-4 could alleviate injury in pancreatic islet cells, and Western blot analysis showed that these GPs upregulated gene expression of the insulin receptor, insulin receptor substrate 2, Akt, and glucose transporter type 4.
28347786	0	8	theme	bioactive	71:79	arg1	perspective					81:91	A bioactive perspective	69:91	A bioactive perspective	69:91	Consumption of guava may have beneficial effects in type 2 diabetes: A bioactive perspective.
28347786	4	9	theme	insulin	825:831	arg1	sensitivity					833:843	insulin sensitivity	825:843	insulin sensitivity	825:843	In addition, GPs efficiently decreased levels of fasting blood glucose, glucosylated serum protein, serum insulin, homeostasis model assessment of insulin resistance, total cholesterol, triglyceride and serum alanine transaminase, improved oral glucose tolerance, and increased insulin sensitivity in rats with T2DM.
28347786	5	10	theme	Akt	1112:1114	arg1	expression					1046:1055	gene expression	1041:1055	gene expression of the insulin receptor, insulin receptor substrate 2, Akt, and glucose transporter type 4	1041:1146	Histopathological observations suggested that GP-1, GP-3, and GP-4 could alleviate injury in pancreatic islet cells, and Western blot analysis showed that these GPs upregulated gene expression of the insulin receptor, insulin receptor substrate 2, Akt, and glucose transporter type 4.
28347786	4	11	theme	resistance	702:711	arg1	triglyceride					733:744	triglyceride	733:744	triglyceride	733:744	In addition, GPs efficiently decreased levels of fasting blood glucose, glucosylated serum protein, serum insulin, homeostasis model assessment of insulin resistance, total cholesterol, triglyceride and serum alanine transaminase, improved oral glucose tolerance, and increased insulin sensitivity in rats with T2DM.
28347786	4	11	theme	resistance	702:711	arg1	insulin					653:659	serum insulin	647:659	serum insulin	647:659	In addition, GPs efficiently decreased levels of fasting blood glucose, glucosylated serum protein, serum insulin, homeostasis model assessment of insulin resistance, total cholesterol, triglyceride and serum alanine transaminase, improved oral glucose tolerance, and increased insulin sensitivity in rats with T2DM.
28347786	4	11	theme	resistance	702:711	arg1	protein					638:644	glucosylated serum protein	619:644	glucosylated serum protein	619:644	In addition, GPs efficiently decreased levels of fasting blood glucose, glucosylated serum protein, serum insulin, homeostasis model assessment of insulin resistance, total cholesterol, triglyceride and serum alanine transaminase, improved oral glucose tolerance, and increased insulin sensitivity in rats with T2DM.
28347786	4	11	theme	resistance	702:711	arg1	transaminase					764:775	serum alanine transaminase	750:775	serum alanine transaminase	750:775	In addition, GPs efficiently decreased levels of fasting blood glucose, glucosylated serum protein, serum insulin, homeostasis model assessment of insulin resistance, total cholesterol, triglyceride and serum alanine transaminase, improved oral glucose tolerance, and increased insulin sensitivity in rats with T2DM.
28347786	4	11	theme	resistance	702:711	arg1	assessment					680:689	homeostasis model assessment	662:689	homeostasis model assessment of insulin resistance	662:711	In addition, GPs efficiently decreased levels of fasting blood glucose, glucosylated serum protein, serum insulin, homeostasis model assessment of insulin resistance, total cholesterol, triglyceride and serum alanine transaminase, improved oral glucose tolerance, and increased insulin sensitivity in rats with T2DM.
28347786	4	11	theme	resistance	702:711	arg1	cholesterol					720:730	total cholesterol	714:730	total cholesterol	714:730	In addition, GPs efficiently decreased levels of fasting blood glucose, glucosylated serum protein, serum insulin, homeostasis model assessment of insulin resistance, total cholesterol, triglyceride and serum alanine transaminase, improved oral glucose tolerance, and increased insulin sensitivity in rats with T2DM.
28347786	4	11	theme	resistance	702:711	arg1	glucose					610:616	fasting blood glucose	596:616	fasting blood glucose	596:616	In addition, GPs efficiently decreased levels of fasting blood glucose, glucosylated serum protein, serum insulin, homeostasis model assessment of insulin resistance, total cholesterol, triglyceride and serum alanine transaminase, improved oral glucose tolerance, and increased insulin sensitivity in rats with T2DM.
28347786	4	12	theme	assessment	680:689	arg1	levels					586:591	levels	586:591	levels of fasting blood glucose, glucosylated serum protein, serum insulin, homeostasis model assessment of insulin resistance, total cholesterol, triglyceride and serum alanine transaminase	586:775	In addition, GPs efficiently decreased levels of fasting blood glucose, glucosylated serum protein, serum insulin, homeostasis model assessment of insulin resistance, total cholesterol, triglyceride and serum alanine transaminase, improved oral glucose tolerance, and increased insulin sensitivity in rats with T2DM.
28347786	5	13	theme	type	1141:1144	arg1	expression					1046:1055	gene expression	1041:1055	gene expression of the insulin receptor, insulin receptor substrate 2, Akt, and glucose transporter type 4	1041:1146	Histopathological observations suggested that GP-1, GP-3, and GP-4 could alleviate injury in pancreatic islet cells, and Western blot analysis showed that these GPs upregulated gene expression of the insulin receptor, insulin receptor substrate 2, Akt, and glucose transporter type 4.
28347786	6	14	theme	vascular	1269:1276	arg1	disease					1278:1284	vascular disease	1269:1284	vascular disease	1269:1284	Taken together, these data suggest that GPs may be beneficial in treating T2DM and reducing the risk of hyperlipidemia, vascular disease, and cirrhosis via the PI3K/Akt signaling pathway.
28347786	1	15	from	mechanisms	203:212	arg1	rats					248:251	rats	248:251	rats with type 2 diabetic mellitus (T2DM)	248:288	The objectives of this study were to evaluate the anti-diabetic and anti-hyperlipidemic effects and relative mechanisms of guava polysaccharides (GPs) in rats with type 2 diabetic mellitus (T2DM).
28347786	0	16	contain	have	25:28	arg1	Consumption					0:10	Consumption	0:10	Consumption of guava	0:19	Consumption of guava may have beneficial effects in type 2 diabetes: A bioactive perspective.
28347786	0	16	contain	have	25:28	arg2	effects					41:47	beneficial effects	30:47	beneficial effects	30:47	Consumption of guava may have beneficial effects in type 2 diabetes: A bioactive perspective.
28347786	4	17	theme	insulin	694:700	arg1	resistance					702:711	insulin resistance	694:711	insulin resistance	694:711	In addition, GPs efficiently decreased levels of fasting blood glucose, glucosylated serum protein, serum insulin, homeostasis model assessment of insulin resistance, total cholesterol, triglyceride and serum alanine transaminase, improved oral glucose tolerance, and increased insulin sensitivity in rats with T2DM.
28347786	5	18	from	injury	947:952	arg1	cells					974:978	pancreatic islet cells	957:978	pancreatic islet cells	957:978	Histopathological observations suggested that GP-1, GP-3, and GP-4 could alleviate injury in pancreatic islet cells, and Western blot analysis showed that these GPs upregulated gene expression of the insulin receptor, insulin receptor substrate 2, Akt, and glucose transporter type 4.
28347786	5	19	theme	gene	1041:1044	arg1	expression					1046:1055	gene expression	1041:1055	gene expression of the insulin receptor, insulin receptor substrate 2, Akt, and glucose transporter type 4	1041:1146	Histopathological observations suggested that GP-1, GP-3, and GP-4 could alleviate injury in pancreatic islet cells, and Western blot analysis showed that these GPs upregulated gene expression of the insulin receptor, insulin receptor substrate 2, Akt, and glucose transporter type 4.
28347786	4	20	theme	oral	787:790	arg1	tolerance					800:808	oral glucose tolerance	787:808	oral glucose tolerance	787:808	In addition, GPs efficiently decreased levels of fasting blood glucose, glucosylated serum protein, serum insulin, homeostasis model assessment of insulin resistance, total cholesterol, triglyceride and serum alanine transaminase, improved oral glucose tolerance, and increased insulin sensitivity in rats with T2DM.
28347786	4	21	theme	total	714:718	arg1	cholesterol					720:730	total cholesterol	714:730	total cholesterol	714:730	In addition, GPs efficiently decreased levels of fasting blood glucose, glucosylated serum protein, serum insulin, homeostasis model assessment of insulin resistance, total cholesterol, triglyceride and serum alanine transaminase, improved oral glucose tolerance, and increased insulin sensitivity in rats with T2DM.
28347786	4	22	theme	fasting	596:602	arg1	glucose					610:616	fasting blood glucose	596:616	fasting blood glucose	596:616	In addition, GPs efficiently decreased levels of fasting blood glucose, glucosylated serum protein, serum insulin, homeostasis model assessment of insulin resistance, total cholesterol, triglyceride and serum alanine transaminase, improved oral glucose tolerance, and increased insulin sensitivity in rats with T2DM.
28347786	6	23	theme	hyperlipidemia	1253:1266	arg1	risk					1245:1248	the risk	1241:1248	the risk of hyperlipidemia, vascular disease, and cirrhosis	1241:1299	Taken together, these data suggest that GPs may be beneficial in treating T2DM and reducing the risk of hyperlipidemia, vascular disease, and cirrhosis via the PI3K/Akt signaling pathway.
28347786	1	24	theme	anti-diabetic	144:156	arg1	effects					182:188	anti-diabetic and anti-hyperlipidemic effects	144:188	anti-diabetic and anti-hyperlipidemic effects	144:188	The objectives of this study were to evaluate the anti-diabetic and anti-hyperlipidemic effects and relative mechanisms of guava polysaccharides (GPs) in rats with type 2 diabetic mellitus (T2DM).
28347786	4	25	theme	model	674:678	arg1	assessment					680:689	homeostasis model assessment	662:689	homeostasis model assessment of insulin resistance	662:711	In addition, GPs efficiently decreased levels of fasting blood glucose, glucosylated serum protein, serum insulin, homeostasis model assessment of insulin resistance, total cholesterol, triglyceride and serum alanine transaminase, improved oral glucose tolerance, and increased insulin sensitivity in rats with T2DM.
28347786	4	26	theme	glucose	610:616	arg1	levels					586:591	levels	586:591	levels of fasting blood glucose, glucosylated serum protein, serum insulin, homeostasis model assessment of insulin resistance, total cholesterol, triglyceride and serum alanine transaminase	586:775	In addition, GPs efficiently decreased levels of fasting blood glucose, glucosylated serum protein, serum insulin, homeostasis model assessment of insulin resistance, total cholesterol, triglyceride and serum alanine transaminase, improved oral glucose tolerance, and increased insulin sensitivity in rats with T2DM.
28347786	4	27	theme	glucose	792:798	arg1	tolerance					800:808	oral glucose tolerance	787:808	oral glucose tolerance	787:808	In addition, GPs efficiently decreased levels of fasting blood glucose, glucosylated serum protein, serum insulin, homeostasis model assessment of insulin resistance, total cholesterol, triglyceride and serum alanine transaminase, improved oral glucose tolerance, and increased insulin sensitivity in rats with T2DM.
28347786	4	28	theme	homeostasis	662:672	arg1	assessment					680:689	homeostasis model assessment	662:689	homeostasis model assessment of insulin resistance	662:711	In addition, GPs efficiently decreased levels of fasting blood glucose, glucosylated serum protein, serum insulin, homeostasis model assessment of insulin resistance, total cholesterol, triglyceride and serum alanine transaminase, improved oral glucose tolerance, and increased insulin sensitivity in rats with T2DM.
28347786	1	29	theme	anti-hyperlipidemic	162:180	arg1	effects					182:188	anti-diabetic and anti-hyperlipidemic effects	144:188	anti-diabetic and anti-hyperlipidemic effects	144:188	The objectives of this study were to evaluate the anti-diabetic and anti-hyperlipidemic effects and relative mechanisms of guava polysaccharides (GPs) in rats with type 2 diabetic mellitus (T2DM).
28347786	1	30	theme	type	258:261	arg1	T2DM					284:287	T2DM	284:287	T2DM	284:287	The objectives of this study were to evaluate the anti-diabetic and anti-hyperlipidemic effects and relative mechanisms of guava polysaccharides (GPs) in rats with type 2 diabetic mellitus (T2DM).
28347786	1	30	theme	type	258:261	arg1	mellitus					274:281	type 2 diabetic mellitus	258:281	type 2 diabetic mellitus (T2DM)	258:288	The objectives of this study were to evaluate the anti-diabetic and anti-hyperlipidemic effects and relative mechanisms of guava polysaccharides (GPs) in rats with type 2 diabetic mellitus (T2DM).
28347786	2	31	dep	characterization	304:319	arg1	The					291:293	The	291:293	The	291:293	The chemical characterization and monosaccharide compositions of GPs, named as GP-1, GP-2, GP-3, and GP-4, were determined by PMP-HPLC and FT-IR.
28347786	4	32	with	rats	848:851	arg1	T2DM					858:861	T2DM	858:861	T2DM	858:861	In addition, GPs efficiently decreased levels of fasting blood glucose, glucosylated serum protein, serum insulin, homeostasis model assessment of insulin resistance, total cholesterol, triglyceride and serum alanine transaminase, improved oral glucose tolerance, and increased insulin sensitivity in rats with T2DM.
28347786	0	33	theme	guava	15:19	arg1	Consumption					0:10	Consumption	0:10	Consumption of guava	0:19	Consumption of guava may have beneficial effects in type 2 diabetes: A bioactive perspective.
28347786	3	34	theme	saccharide	487:496	arg1	absorptions					498:508	the typical saccharide absorptions	475:508	the typical saccharide absorptions	475:508	The results revealed that all GPs had the typical saccharide absorptions, and all were heteropolysaccharides.
28347786	4	35	theme	glucosylated	619:630	arg1	protein					638:644	glucosylated serum protein	619:644	glucosylated serum protein	619:644	In addition, GPs efficiently decreased levels of fasting blood glucose, glucosylated serum protein, serum insulin, homeostasis model assessment of insulin resistance, total cholesterol, triglyceride and serum alanine transaminase, improved oral glucose tolerance, and increased insulin sensitivity in rats with T2DM.
28347786	0	36	theme	beneficial	30:39	arg1	effects					41:47	beneficial effects	30:47	beneficial effects	30:47	Consumption of guava may have beneficial effects in type 2 diabetes: A bioactive perspective.
28347786	1	37	theme	diabetic	265:272	arg1	T2DM					284:287	T2DM	284:287	T2DM	284:287	The objectives of this study were to evaluate the anti-diabetic and anti-hyperlipidemic effects and relative mechanisms of guava polysaccharides (GPs) in rats with type 2 diabetic mellitus (T2DM).
28347786	1	37	theme	diabetic	265:272	arg1	mellitus					274:281	type 2 diabetic mellitus	258:281	type 2 diabetic mellitus (T2DM)	258:288	The objectives of this study were to evaluate the anti-diabetic and anti-hyperlipidemic effects and relative mechanisms of guava polysaccharides (GPs) in rats with type 2 diabetic mellitus (T2DM).
28347786	4	38	theme	alanine	756:762	arg1	transaminase					764:775	serum alanine transaminase	750:775	serum alanine transaminase	750:775	In addition, GPs efficiently decreased levels of fasting blood glucose, glucosylated serum protein, serum insulin, homeostasis model assessment of insulin resistance, total cholesterol, triglyceride and serum alanine transaminase, improved oral glucose tolerance, and increased insulin sensitivity in rats with T2DM.
28347786	2	39	theme	GPs	356:358	arg1	characterization					304:319	chemical characterization	295:319	chemical characterization	295:319	The chemical characterization and monosaccharide compositions of GPs, named as GP-1, GP-2, GP-3, and GP-4, were determined by PMP-HPLC and FT-IR.
28347786	2	39	theme	GPs	356:358	arg1	compositions					340:351	monosaccharide compositions	325:351	monosaccharide compositions	325:351	The chemical characterization and monosaccharide compositions of GPs, named as GP-1, GP-2, GP-3, and GP-4, were determined by PMP-HPLC and FT-IR.
28347786	1	40	theme	relative	194:201	arg1	mechanisms					203:212	relative mechanisms	194:212	relative mechanisms	194:212	The objectives of this study were to evaluate the anti-diabetic and anti-hyperlipidemic effects and relative mechanisms of guava polysaccharides (GPs) in rats with type 2 diabetic mellitus (T2DM).
28347786	6	41	theme	signaling	1318:1326	arg1	pathway					1328:1334	the PI3K/Akt signaling pathway	1305:1334	the PI3K/Akt signaling pathway	1305:1334	Taken together, these data suggest that GPs may be beneficial in treating T2DM and reducing the risk of hyperlipidemia, vascular disease, and cirrhosis via the PI3K/Akt signaling pathway.
28347786	5	42	theme	insulin	1082:1088	arg1	substrate					1099:1107	insulin receptor substrate 2	1082:1109	insulin receptor substrate 2	1082:1109	Histopathological observations suggested that GP-1, GP-3, and GP-4 could alleviate injury in pancreatic islet cells, and Western blot analysis showed that these GPs upregulated gene expression of the insulin receptor, insulin receptor substrate 2, Akt, and glucose transporter type 4.
28347786	4	43	theme	serum	750:754	arg1	transaminase					764:775	serum alanine transaminase	750:775	serum alanine transaminase	750:775	In addition, GPs efficiently decreased levels of fasting blood glucose, glucosylated serum protein, serum insulin, homeostasis model assessment of insulin resistance, total cholesterol, triglyceride and serum alanine transaminase, improved oral glucose tolerance, and increased insulin sensitivity in rats with T2DM.
28347786	5	44	theme	pancreatic	957:966	arg1	cells					974:978	pancreatic islet cells	957:978	pancreatic islet cells	957:978	Histopathological observations suggested that GP-1, GP-3, and GP-4 could alleviate injury in pancreatic islet cells, and Western blot analysis showed that these GPs upregulated gene expression of the insulin receptor, insulin receptor substrate 2, Akt, and glucose transporter type 4.
28347786	6	45	theme	PI3K/Akt	1309:1316	arg1	pathway					1328:1334	the PI3K/Akt signaling pathway	1305:1334	the PI3K/Akt signaling pathway	1305:1334	Taken together, these data suggest that GPs may be beneficial in treating T2DM and reducing the risk of hyperlipidemia, vascular disease, and cirrhosis via the PI3K/Akt signaling pathway.
28347786	5	46	theme	receptor	1090:1097	arg1	substrate					1099:1107	insulin receptor substrate 2	1082:1109	insulin receptor substrate 2	1082:1109	Histopathological observations suggested that GP-1, GP-3, and GP-4 could alleviate injury in pancreatic islet cells, and Western blot analysis showed that these GPs upregulated gene expression of the insulin receptor, insulin receptor substrate 2, Akt, and glucose transporter type 4.
28347786	6	47	theme	disease	1278:1284	arg1	risk					1245:1248	the risk	1241:1248	the risk of hyperlipidemia, vascular disease, and cirrhosis	1241:1299	Taken together, these data suggest that GPs may be beneficial in treating T2DM and reducing the risk of hyperlipidemia, vascular disease, and cirrhosis via the PI3K/Akt signaling pathway.
28347786	5	48	theme	Western	985:991	arg1	analysis					998:1005	Western blot analysis	985:1005	Western blot analysis	985:1005	Histopathological observations suggested that GP-1, GP-3, and GP-4 could alleviate injury in pancreatic islet cells, and Western blot analysis showed that these GPs upregulated gene expression of the insulin receptor, insulin receptor substrate 2, Akt, and glucose transporter type 4.
28347786	1	49	with	rats	248:251	arg1	T2DM					284:287	T2DM	284:287	T2DM	284:287	The objectives of this study were to evaluate the anti-diabetic and anti-hyperlipidemic effects and relative mechanisms of guava polysaccharides (GPs) in rats with type 2 diabetic mellitus (T2DM).
28347786	1	49	with	rats	248:251	arg1	mellitus					274:281	type 2 diabetic mellitus	258:281	type 2 diabetic mellitus (T2DM)	258:288	The objectives of this study were to evaluate the anti-diabetic and anti-hyperlipidemic effects and relative mechanisms of guava polysaccharides (GPs) in rats with type 2 diabetic mellitus (T2DM).
28347786	4	50	theme	serum	647:651	arg1	insulin					653:659	serum insulin	647:659	serum insulin	647:659	In addition, GPs efficiently decreased levels of fasting blood glucose, glucosylated serum protein, serum insulin, homeostasis model assessment of insulin resistance, total cholesterol, triglyceride and serum alanine transaminase, improved oral glucose tolerance, and increased insulin sensitivity in rats with T2DM.
28347786	5	51	theme	substrate	1099:1107	arg1	expression					1046:1055	gene expression	1041:1055	gene expression of the insulin receptor, insulin receptor substrate 2, Akt, and glucose transporter type 4	1041:1146	Histopathological observations suggested that GP-1, GP-3, and GP-4 could alleviate injury in pancreatic islet cells, and Western blot analysis showed that these GPs upregulated gene expression of the insulin receptor, insulin receptor substrate 2, Akt, and glucose transporter type 4.
28347786	4	52	theme	transaminase	764:775	arg1	levels					586:591	levels	586:591	levels of fasting blood glucose, glucosylated serum protein, serum insulin, homeostasis model assessment of insulin resistance, total cholesterol, triglyceride and serum alanine transaminase	586:775	In addition, GPs efficiently decreased levels of fasting blood glucose, glucosylated serum protein, serum insulin, homeostasis model assessment of insulin resistance, total cholesterol, triglyceride and serum alanine transaminase, improved oral glucose tolerance, and increased insulin sensitivity in rats with T2DM.
28347786	1	53	from	effects	182:188	arg1	rats					248:251	rats	248:251	rats with type 2 diabetic mellitus (T2DM)	248:288	The objectives of this study were to evaluate the anti-diabetic and anti-hyperlipidemic effects and relative mechanisms of guava polysaccharides (GPs) in rats with type 2 diabetic mellitus (T2DM).
28347786	2	54	theme	chemical	295:302	arg1	characterization					304:319	chemical characterization	295:319	chemical characterization	295:319	The chemical characterization and monosaccharide compositions of GPs, named as GP-1, GP-2, GP-3, and GP-4, were determined by PMP-HPLC and FT-IR.
28347786	3	55	contain	had	471:473	arg2	absorptions					498:508	the typical saccharide absorptions	475:508	the typical saccharide absorptions	475:508	The results revealed that all GPs had the typical saccharide absorptions, and all were heteropolysaccharides.
28347786	3	55	contain	had	471:473	arg1	GPs					467:469	all GPs	463:469	all GPs	463:469	The results revealed that all GPs had the typical saccharide absorptions, and all were heteropolysaccharides.
28347786	4	56	theme	blood	604:608	arg1	glucose					610:616	fasting blood glucose	596:616	fasting blood glucose	596:616	In addition, GPs efficiently decreased levels of fasting blood glucose, glucosylated serum protein, serum insulin, homeostasis model assessment of insulin resistance, total cholesterol, triglyceride and serum alanine transaminase, improved oral glucose tolerance, and increased insulin sensitivity in rats with T2DM.
28347786	5	57	theme	islet	968:972	arg1	cells					974:978	pancreatic islet cells	957:978	pancreatic islet cells	957:978	Histopathological observations suggested that GP-1, GP-3, and GP-4 could alleviate injury in pancreatic islet cells, and Western blot analysis showed that these GPs upregulated gene expression of the insulin receptor, insulin receptor substrate 2, Akt, and glucose transporter type 4.
28347786	5	58	theme	Histopathological	864:880	arg1	observations					882:893	Histopathological observations	864:893	Histopathological observations	864:893	Histopathological observations suggested that GP-1, GP-3, and GP-4 could alleviate injury in pancreatic islet cells, and Western blot analysis showed that these GPs upregulated gene expression of the insulin receptor, insulin receptor substrate 2, Akt, and glucose transporter type 4.
28347786	3	59	theme	typical	479:485	arg1	absorptions					498:508	the typical saccharide absorptions	475:508	the typical saccharide absorptions	475:508	The results revealed that all GPs had the typical saccharide absorptions, and all were heteropolysaccharides.
28347786	5	60	theme	blot	993:996	arg1	analysis					998:1005	Western blot analysis	985:1005	Western blot analysis	985:1005	Histopathological observations suggested that GP-1, GP-3, and GP-4 could alleviate injury in pancreatic islet cells, and Western blot analysis showed that these GPs upregulated gene expression of the insulin receptor, insulin receptor substrate 2, Akt, and glucose transporter type 4.
28347786	4	61	theme	cholesterol	720:730	arg1	levels					586:591	levels	586:591	levels of fasting blood glucose, glucosylated serum protein, serum insulin, homeostasis model assessment of insulin resistance, total cholesterol, triglyceride and serum alanine transaminase	586:775	In addition, GPs efficiently decreased levels of fasting blood glucose, glucosylated serum protein, serum insulin, homeostasis model assessment of insulin resistance, total cholesterol, triglyceride and serum alanine transaminase, improved oral glucose tolerance, and increased insulin sensitivity in rats with T2DM.
28347786	4	62	theme	insulin	653:659	arg1	levels					586:591	levels	586:591	levels of fasting blood glucose, glucosylated serum protein, serum insulin, homeostasis model assessment of insulin resistance, total cholesterol, triglyceride and serum alanine transaminase	586:775	In addition, GPs efficiently decreased levels of fasting blood glucose, glucosylated serum protein, serum insulin, homeostasis model assessment of insulin resistance, total cholesterol, triglyceride and serum alanine transaminase, improved oral glucose tolerance, and increased insulin sensitivity in rats with T2DM.
28347786	1	63	theme	guava	217:221	arg1	polysaccharides					223:237	guava polysaccharides	217:237	guava polysaccharides (GPs)	217:243	The objectives of this study were to evaluate the anti-diabetic and anti-hyperlipidemic effects and relative mechanisms of guava polysaccharides (GPs) in rats with type 2 diabetic mellitus (T2DM).
28347786	1	63	theme	guava	217:221	arg1	GPs					240:242	GPs	240:242	GPs	240:242	The objectives of this study were to evaluate the anti-diabetic and anti-hyperlipidemic effects and relative mechanisms of guava polysaccharides (GPs) in rats with type 2 diabetic mellitus (T2DM).
28347786	1	64	dep	effects	182:188	arg1	the					140:142	the	140:142	the	140:142	The objectives of this study were to evaluate the anti-diabetic and anti-hyperlipidemic effects and relative mechanisms of guava polysaccharides (GPs) in rats with type 2 diabetic mellitus (T2DM).
28347786	1	65	theme	study	117:121	arg1	objectives					98:107	The objectives	94:107	The objectives of this study	94:121	The objectives of this study were to evaluate the anti-diabetic and anti-hyperlipidemic effects and relative mechanisms of guava polysaccharides (GPs) in rats with type 2 diabetic mellitus (T2DM).
28347786	1	66	theme	polysaccharides	223:237	arg1	effects					182:188	anti-diabetic and anti-hyperlipidemic effects	144:188	anti-diabetic and anti-hyperlipidemic effects	144:188	The objectives of this study were to evaluate the anti-diabetic and anti-hyperlipidemic effects and relative mechanisms of guava polysaccharides (GPs) in rats with type 2 diabetic mellitus (T2DM).
28347786	1	66	theme	polysaccharides	223:237	arg1	mechanisms					203:212	relative mechanisms	194:212	relative mechanisms	194:212	The objectives of this study were to evaluate the anti-diabetic and anti-hyperlipidemic effects and relative mechanisms of guava polysaccharides (GPs) in rats with type 2 diabetic mellitus (T2DM).
28347786	0	67	theme	type	52:55	arg1	diabetes					59:66	type 2 diabetes	52:66	type 2 diabetes	52:66	Consumption of guava may have beneficial effects in type 2 diabetes: A bioactive perspective.
28347786	4	68	theme	triglyceride	733:744	arg1	levels					586:591	levels	586:591	levels of fasting blood glucose, glucosylated serum protein, serum insulin, homeostasis model assessment of insulin resistance, total cholesterol, triglyceride and serum alanine transaminase	586:775	In addition, GPs efficiently decreased levels of fasting blood glucose, glucosylated serum protein, serum insulin, homeostasis model assessment of insulin resistance, total cholesterol, triglyceride and serum alanine transaminase, improved oral glucose tolerance, and increased insulin sensitivity in rats with T2DM.
23670237	0	0	theme	wall	68:71	arg1	changes					85:91	cell wall composition changes	63:91	cell wall composition changes	63:91	Effects of freezing and thawing on texture, microstructure and cell wall composition changes in papaya tissues.
23670237	7	1	from	decrease	1089:1096	arg1	mass					1115:1118	the molecular mass	1101:1118	the molecular mass of pectic and hemicellulosic polymers in frozen-thawed papayas using high-performance size-exclusion chromatography	1101:1234	This was due to a decrease in the molecular mass of pectic and hemicellulosic polymers in frozen-thawed papayas using high-performance size-exclusion chromatography.
23670237	8	2	theme	tissues	1413:1419	arg1	firmness					1394:1401	firmness	1394:1401	firmness of papaya tissues	1394:1419	CONCLUSION The freezing and thawing processes caused fine structural damage and cell wall composition changes which contributed to a loss of drip volume and firmness of papaya tissues.
23670237	8	2	theme	tissues	1413:1419	arg1	loss					1370:1373	a loss	1368:1373	a loss of drip volume	1368:1388	CONCLUSION The freezing and thawing processes caused fine structural damage and cell wall composition changes which contributed to a loss of drip volume and firmness of papaya tissues.
23670237	1	3	theme	frozen	139:144	arg1	fruit					146:150	frozen fruit	139:150	frozen fruit	139:150	BACKGROUND During storage, frozen fruit may be thawed and refrozen many times before consumption, which may be extremely damaging to the texture of the frozen fruit and reverse the advantage of fast freezing.
23670237	3	4	from	increase	496:503	arg1	firmness					536:543	firmness	536:543	firmness	536:543	RESULTS The frozen-thawed papayas had an increase in drip loss and a decrease in firmness with increasing number of freeze-thaw cycles.
23670237	3	4	from	increase	496:503	arg1	loss					513:516	drip loss	508:516	drip loss	508:516	RESULTS The frozen-thawed papayas had an increase in drip loss and a decrease in firmness with increasing number of freeze-thaw cycles.
23670237	6	5	theme	24	978:979	arg1	%					980:980	%	980:980	%	980:980	These changes related with a significant decrease in alcohol-insoluble solids, Na₂CO₃- and 24% KOH-soluble fractions and an increase in the water-, EDTA- and 4% KOH-soluble fractions.
23670237	0	6	theme	cell	63:66	arg1	changes					85:91	cell wall composition changes	63:91	cell wall composition changes	63:91	Effects of freezing and thawing on texture, microstructure and cell wall composition changes in papaya tissues.
23670237	3	7	contain	had	489:491	arg2	decrease					524:531	a decrease	522:531	a decrease in firmness	522:543	RESULTS The frozen-thawed papayas had an increase in drip loss and a decrease in firmness with increasing number of freeze-thaw cycles.
23670237	3	7	contain	had	489:491	arg1	papayas					481:487	The frozen-thawed papayas	463:487	The frozen-thawed papayas	463:487	RESULTS The frozen-thawed papayas had an increase in drip loss and a decrease in firmness with increasing number of freeze-thaw cycles.
23670237	3	7	contain	had	489:491	arg2	increase					496:503	an increase	493:503	an increase in drip loss	493:516	RESULTS The frozen-thawed papayas had an increase in drip loss and a decrease in firmness with increasing number of freeze-thaw cycles.
23670237	8	8	dep	freezing	1252:1259	arg1	The					1248:1250	The	1248:1250	The	1248:1250	CONCLUSION The freezing and thawing processes caused fine structural damage and cell wall composition changes which contributed to a loss of drip volume and firmness of papaya tissues.
23670237	8	8	dep	freezing	1252:1259	arg1	processes					1273:1281	processes	1273:1281	processes	1273:1281	CONCLUSION The freezing and thawing processes caused fine structural damage and cell wall composition changes which contributed to a loss of drip volume and firmness of papaya tissues.
23670237	3	9	theme	frozen-thawed	467:479	arg1	papayas					481:487	The frozen-thawed papayas	463:487	The frozen-thawed papayas	463:487	RESULTS The frozen-thawed papayas had an increase in drip loss and a decrease in firmness with increasing number of freeze-thaw cycles.
23670237	7	10	theme	hemicellulosic	1134:1147	arg1	polymers					1149:1156	pectic and hemicellulosic polymers	1123:1156	polymers	1149:1156	This was due to a decrease in the molecular mass of pectic and hemicellulosic polymers in frozen-thawed papayas using high-performance size-exclusion chromatography.
23670237	0	11	from	Effects	0:6	arg1	changes					85:91	cell wall composition changes	63:91	cell wall composition changes	63:91	Effects of freezing and thawing on texture, microstructure and cell wall composition changes in papaya tissues.
23670237	0	11	from	Effects	0:6	arg1	microstructure					44:57	microstructure	44:57	microstructure	44:57	Effects of freezing and thawing on texture, microstructure and cell wall composition changes in papaya tissues.
23670237	0	11	from	Effects	0:6	arg1	texture					35:41	texture	35:41	texture	35:41	Effects of freezing and thawing on texture, microstructure and cell wall composition changes in papaya tissues.
23670237	1	12	dep	BACKGROUND	112:121	arg1	thawed					159:164	thawed	159:164	thawed	159:164	BACKGROUND During storage, frozen fruit may be thawed and refrozen many times before consumption, which may be extremely damaging to the texture of the frozen fruit and reverse the advantage of fast freezing.
23670237	1	12	dep	BACKGROUND	112:121	arg1	refrozen					170:177	refrozen	170:177	refrozen many times before consumption, which may be extremely damaging to the texture of the frozen fruit and reverse the advantage of fast freezing	170:318	BACKGROUND During storage, frozen fruit may be thawed and refrozen many times before consumption, which may be extremely damaging to the texture of the frozen fruit and reverse the advantage of fast freezing.
23670237	4	13	theme	electron	715:722	arg1	microscopy					724:733	transmission electron microscopy	702:733	transmission electron microscopy	702:733	Light microscopy showed irregular shapes and cell damage in parenchyma cells of frozen-thawed papayas, whereas transmission electron microscopy showed loss of cell wall materials in middle lamella.
23670237	0	14	theme	composition	73:83	arg1	changes					85:91	cell wall composition changes	63:91	cell wall composition changes	63:91	Effects of freezing and thawing on texture, microstructure and cell wall composition changes in papaya tissues.
23670237	1	15	theme	freezing	311:318	arg1	advantage					293:301	the advantage	289:301	the advantage of fast freezing	289:318	BACKGROUND During storage, frozen fruit may be thawed and refrozen many times before consumption, which may be extremely damaging to the texture of the frozen fruit and reverse the advantage of fast freezing.
23670237	8	16	theme	wall	1322:1325	arg1	changes					1339:1345	cell wall composition changes	1317:1345	cell wall composition changes which contributed to a loss of drip volume and firmness of papaya tissues	1317:1419	CONCLUSION The freezing and thawing processes caused fine structural damage and cell wall composition changes which contributed to a loss of drip volume and firmness of papaya tissues.
23670237	4	17	from	loss	742:745	arg1	lamella					780:786	middle lamella	773:786	middle lamella	773:786	Light microscopy showed irregular shapes and cell damage in parenchyma cells of frozen-thawed papayas, whereas transmission electron microscopy showed loss of cell wall materials in middle lamella.
23670237	3	18	theme	drip	508:511	arg1	loss					513:516	drip loss	508:516	drip loss	508:516	RESULTS The frozen-thawed papayas had an increase in drip loss and a decrease in firmness with increasing number of freeze-thaw cycles.
23670237	0	19	theme	papaya	96:101	arg1	tissues					103:109	papaya tissues	96:109	papaya tissues	96:109	Effects of freezing and thawing on texture, microstructure and cell wall composition changes in papaya tissues.
23670237	0	20	from	texture	35:41	arg1	tissues					103:109	papaya tissues	96:109	papaya tissues	96:109	Effects of freezing and thawing on texture, microstructure and cell wall composition changes in papaya tissues.
23670237	4	21	theme	cell	750:753	arg1	materials					760:768	cell wall materials	750:768	cell wall materials	750:768	Light microscopy showed irregular shapes and cell damage in parenchyma cells of frozen-thawed papayas, whereas transmission electron microscopy showed loss of cell wall materials in middle lamella.
23670237	4	22	theme	cell	636:639	arg1	damage					641:646	cell damage	636:646	cell damage	636:646	Light microscopy showed irregular shapes and cell damage in parenchyma cells of frozen-thawed papayas, whereas transmission electron microscopy showed loss of cell wall materials in middle lamella.
23670237	2	23	theme	thawing	349:355	arg1	effects					325:331	The effects	321:331	The effects of freezing and thawing on texture, microstructure and cell wall composition changes in papaya tissues	321:434	The effects of freezing and thawing on texture, microstructure and cell wall composition changes in papaya tissues were investigated.
23670237	5	24	theme	freeze-thaw	867:877	arg1	cycles					879:884	five freeze-thaw cycles	862:884	five freeze-thaw cycles	862:884	Moreover, destruction of cell wall was observed after being subjected to five freeze-thaw cycles.
23670237	6	25	from	increase	1011:1018	arg1	fractions					1060:1068	the water-, EDTA- and 4% KOH-soluble fractions	1023:1068	the water-, EDTA- and 4% KOH-soluble fractions	1023:1068	These changes related with a significant decrease in alcohol-insoluble solids, Na₂CO₃- and 24% KOH-soluble fractions and an increase in the water-, EDTA- and 4% KOH-soluble fractions.
23670237	6	25	from	increase	1011:1018	arg1	fractions					994:1002	24% KOH-soluble fractions	978:1002	24% KOH-soluble fractions	978:1002	These changes related with a significant decrease in alcohol-insoluble solids, Na₂CO₃- and 24% KOH-soluble fractions and an increase in the water-, EDTA- and 4% KOH-soluble fractions.
23670237	6	25	from	increase	1011:1018	arg1	Na₂CO₃-					966:972	Na₂CO₃-	966:972	Na₂CO₃-	966:972	These changes related with a significant decrease in alcohol-insoluble solids, Na₂CO₃- and 24% KOH-soluble fractions and an increase in the water-, EDTA- and 4% KOH-soluble fractions.
23670237	6	25	from	increase	1011:1018	arg1	solids					958:963	alcohol-insoluble solids	940:963	alcohol-insoluble solids	940:963	These changes related with a significant decrease in alcohol-insoluble solids, Na₂CO₃- and 24% KOH-soluble fractions and an increase in the water-, EDTA- and 4% KOH-soluble fractions.
23670237	3	26	dep	RESULTS	455:461	arg1	had					489:491	had	489:491	had an increase in drip loss and a decrease in firmness with increasing number of freeze-thaw cycles	489:588	RESULTS The frozen-thawed papayas had an increase in drip loss and a decrease in firmness with increasing number of freeze-thaw cycles.
23670237	8	27	theme	drip	1378:1381	arg1	volume					1383:1388	drip volume	1378:1388	drip volume	1378:1388	CONCLUSION The freezing and thawing processes caused fine structural damage and cell wall composition changes which contributed to a loss of drip volume and firmness of papaya tissues.
23670237	4	28	theme	wall	755:758	arg1	materials					760:768	cell wall materials	750:768	cell wall materials	750:768	Light microscopy showed irregular shapes and cell damage in parenchyma cells of frozen-thawed papayas, whereas transmission electron microscopy showed loss of cell wall materials in middle lamella.
23670237	2	29	theme	composition	398:408	arg1	changes					410:416	cell wall composition changes	388:416	cell wall composition changes	388:416	The effects of freezing and thawing on texture, microstructure and cell wall composition changes in papaya tissues were investigated.
23670237	7	30	theme	frozen-thawed	1161:1173	arg1	papayas					1175:1181	frozen-thawed papayas	1161:1181	frozen-thawed papayas using high-performance size-exclusion chromatography	1161:1234	This was due to a decrease in the molecular mass of pectic and hemicellulosic polymers in frozen-thawed papayas using high-performance size-exclusion chromatography.
23670237	4	31	theme	irregular	615:623	arg1	shapes					625:630	irregular shapes	615:630	irregular shapes	615:630	Light microscopy showed irregular shapes and cell damage in parenchyma cells of frozen-thawed papayas, whereas transmission electron microscopy showed loss of cell wall materials in middle lamella.
23670237	4	32	theme	transmission	702:713	arg1	microscopy					724:733	transmission electron microscopy	702:733	transmission electron microscopy	702:733	Light microscopy showed irregular shapes and cell damage in parenchyma cells of frozen-thawed papayas, whereas transmission electron microscopy showed loss of cell wall materials in middle lamella.
23670237	2	33	from	changes	410:416	arg1	tissues					428:434	papaya tissues	421:434	papaya tissues	421:434	The effects of freezing and thawing on texture, microstructure and cell wall composition changes in papaya tissues were investigated.
23670237	2	34	theme	freezing	336:343	arg1	effects					325:331	The effects	321:331	The effects of freezing and thawing on texture, microstructure and cell wall composition changes in papaya tissues	321:434	The effects of freezing and thawing on texture, microstructure and cell wall composition changes in papaya tissues were investigated.
23670237	2	35	theme	wall	393:396	arg1	changes					410:416	cell wall composition changes	388:416	cell wall composition changes	388:416	The effects of freezing and thawing on texture, microstructure and cell wall composition changes in papaya tissues were investigated.
23670237	2	36	from	effects	325:331	arg1	texture					360:366	texture	360:366	texture	360:366	The effects of freezing and thawing on texture, microstructure and cell wall composition changes in papaya tissues were investigated.
23670237	2	36	from	effects	325:331	arg1	changes					410:416	cell wall composition changes	388:416	cell wall composition changes	388:416	The effects of freezing and thawing on texture, microstructure and cell wall composition changes in papaya tissues were investigated.
23670237	2	36	from	effects	325:331	arg1	microstructure					369:382	microstructure	369:382	microstructure	369:382	The effects of freezing and thawing on texture, microstructure and cell wall composition changes in papaya tissues were investigated.
23670237	6	37	theme	water-	1027:1032	arg1	fractions					1060:1068	the water-, EDTA- and 4% KOH-soluble fractions	1023:1068	the water-, EDTA- and 4% KOH-soluble fractions	1023:1068	These changes related with a significant decrease in alcohol-insoluble solids, Na₂CO₃- and 24% KOH-soluble fractions and an increase in the water-, EDTA- and 4% KOH-soluble fractions.
23670237	2	38	theme	cell	388:391	arg1	changes					410:416	cell wall composition changes	388:416	cell wall composition changes	388:416	The effects of freezing and thawing on texture, microstructure and cell wall composition changes in papaya tissues were investigated.
23670237	6	39	theme	alcohol-insoluble	940:956	arg1	solids					958:963	alcohol-insoluble solids	940:963	alcohol-insoluble solids	940:963	These changes related with a significant decrease in alcohol-insoluble solids, Na₂CO₃- and 24% KOH-soluble fractions and an increase in the water-, EDTA- and 4% KOH-soluble fractions.
23670237	6	40	theme	EDTA-	1035:1039	arg1	fractions					1060:1068	the water-, EDTA- and 4% KOH-soluble fractions	1023:1068	the water-, EDTA- and 4% KOH-soluble fractions	1023:1068	These changes related with a significant decrease in alcohol-insoluble solids, Na₂CO₃- and 24% KOH-soluble fractions and an increase in the water-, EDTA- and 4% KOH-soluble fractions.
23670237	4	41	theme	Light	591:595	arg1	microscopy					597:606	Light microscopy	591:606	Light microscopy	591:606	Light microscopy showed irregular shapes and cell damage in parenchyma cells of frozen-thawed papayas, whereas transmission electron microscopy showed loss of cell wall materials in middle lamella.
23670237	0	42	theme	freezing	11:18	arg1	Effects					0:6	Effects	0:6	Effects of freezing and thawing on texture, microstructure and cell wall composition changes in papaya tissues.	0:110	Effects of freezing and thawing on texture, microstructure and cell wall composition changes in papaya tissues.
23670237	6	43	from	decrease	928:935	arg1	fractions					1060:1068	the water-, EDTA- and 4% KOH-soluble fractions	1023:1068	the water-, EDTA- and 4% KOH-soluble fractions	1023:1068	These changes related with a significant decrease in alcohol-insoluble solids, Na₂CO₃- and 24% KOH-soluble fractions and an increase in the water-, EDTA- and 4% KOH-soluble fractions.
23670237	6	43	from	decrease	928:935	arg1	fractions					994:1002	24% KOH-soluble fractions	978:1002	24% KOH-soluble fractions	978:1002	These changes related with a significant decrease in alcohol-insoluble solids, Na₂CO₃- and 24% KOH-soluble fractions and an increase in the water-, EDTA- and 4% KOH-soluble fractions.
23670237	6	43	from	decrease	928:935	arg1	Na₂CO₃-					966:972	Na₂CO₃-	966:972	Na₂CO₃-	966:972	These changes related with a significant decrease in alcohol-insoluble solids, Na₂CO₃- and 24% KOH-soluble fractions and an increase in the water-, EDTA- and 4% KOH-soluble fractions.
23670237	6	43	from	decrease	928:935	arg1	solids					958:963	alcohol-insoluble solids	940:963	alcohol-insoluble solids	940:963	These changes related with a significant decrease in alcohol-insoluble solids, Na₂CO₃- and 24% KOH-soluble fractions and an increase in the water-, EDTA- and 4% KOH-soluble fractions.
23670237	4	44	theme	parenchyma	651:660	arg1	cells					662:666	parenchyma cells	651:666	parenchyma cells of frozen-thawed papayas	651:691	Light microscopy showed irregular shapes and cell damage in parenchyma cells of frozen-thawed papayas, whereas transmission electron microscopy showed loss of cell wall materials in middle lamella.
23670237	1	45	theme	many	179:182	arg1	times					184:188	many times	179:188	many times before consumption, which may be extremely damaging to the texture of the frozen fruit and reverse the advantage of fast freezing	179:318	BACKGROUND During storage, frozen fruit may be thawed and refrozen many times before consumption, which may be extremely damaging to the texture of the frozen fruit and reverse the advantage of fast freezing.
23670237	4	46	dep	showed	608:613	arg1	whereas					694:700	whereas	694:700	whereas	694:700	Light microscopy showed irregular shapes and cell damage in parenchyma cells of frozen-thawed papayas, whereas transmission electron microscopy showed loss of cell wall materials in middle lamella.
23670237	5	47	theme	cell	814:817	arg1	wall					819:822	cell wall	814:822	cell wall	814:822	Moreover, destruction of cell wall was observed after being subjected to five freeze-thaw cycles.
23670237	2	48	theme	papaya	421:426	arg1	tissues					428:434	papaya tissues	421:434	papaya tissues	421:434	The effects of freezing and thawing on texture, microstructure and cell wall composition changes in papaya tissues were investigated.
23670237	1	49	theme	frozen	264:269	arg1	fruit					271:275	the frozen fruit	260:275	the frozen fruit	260:275	BACKGROUND During storage, frozen fruit may be thawed and refrozen many times before consumption, which may be extremely damaging to the texture of the frozen fruit and reverse the advantage of fast freezing.
23670237	0	50	theme	thawing	24:30	arg1	Effects					0:6	Effects	0:6	Effects of freezing and thawing on texture, microstructure and cell wall composition changes in papaya tissues.	0:110	Effects of freezing and thawing on texture, microstructure and cell wall composition changes in papaya tissues.
23670237	8	51	theme	structural	1295:1304	arg1	damage					1306:1311	fine structural damage	1290:1311	fine structural damage	1290:1311	CONCLUSION The freezing and thawing processes caused fine structural damage and cell wall composition changes which contributed to a loss of drip volume and firmness of papaya tissues.
23670237	5	52	theme	wall	819:822	arg1	destruction					799:809	destruction	799:809	destruction of cell wall	799:822	Moreover, destruction of cell wall was observed after being subjected to five freeze-thaw cycles.
23670237	1	53	theme	fruit	271:275	arg1	texture					249:255	the texture	245:255	the texture of the frozen fruit	245:275	BACKGROUND During storage, frozen fruit may be thawed and refrozen many times before consumption, which may be extremely damaging to the texture of the frozen fruit and reverse the advantage of fast freezing.
23670237	8	54	theme	composition	1327:1337	arg1	changes					1339:1345	cell wall composition changes	1317:1345	cell wall composition changes which contributed to a loss of drip volume and firmness of papaya tissues	1317:1419	CONCLUSION The freezing and thawing processes caused fine structural damage and cell wall composition changes which contributed to a loss of drip volume and firmness of papaya tissues.
23670237	0	55	from	microstructure	44:57	arg1	tissues					103:109	papaya tissues	96:109	papaya tissues	96:109	Effects of freezing and thawing on texture, microstructure and cell wall composition changes in papaya tissues.
23670237	7	56	from	mass	1115:1118	arg1	papayas					1175:1181	frozen-thawed papayas	1161:1181	frozen-thawed papayas using high-performance size-exclusion chromatography	1161:1234	This was due to a decrease in the molecular mass of pectic and hemicellulosic polymers in frozen-thawed papayas using high-performance size-exclusion chromatography.
23670237	4	57	theme	frozen-thawed	671:683	arg1	papayas					685:691	frozen-thawed papayas	671:691	frozen-thawed papayas	671:691	Light microscopy showed irregular shapes and cell damage in parenchyma cells of frozen-thawed papayas, whereas transmission electron microscopy showed loss of cell wall materials in middle lamella.
23670237	1	58	theme	fast	306:309	arg1	freezing					311:318	fast freezing	306:318	fast freezing	306:318	BACKGROUND During storage, frozen fruit may be thawed and refrozen many times before consumption, which may be extremely damaging to the texture of the frozen fruit and reverse the advantage of fast freezing.
23670237	7	59	theme	size-exclusion	1206:1219	arg1	chromatography					1221:1234	high-performance size-exclusion chromatography	1189:1234	high-performance size-exclusion chromatography	1189:1234	This was due to a decrease in the molecular mass of pectic and hemicellulosic polymers in frozen-thawed papayas using high-performance size-exclusion chromatography.
23670237	8	60	theme	volume	1383:1388	arg1	firmness					1394:1401	firmness	1394:1401	firmness of papaya tissues	1394:1419	CONCLUSION The freezing and thawing processes caused fine structural damage and cell wall composition changes which contributed to a loss of drip volume and firmness of papaya tissues.
23670237	8	60	theme	volume	1383:1388	arg1	loss					1370:1373	a loss	1368:1373	a loss of drip volume	1368:1388	CONCLUSION The freezing and thawing processes caused fine structural damage and cell wall composition changes which contributed to a loss of drip volume and firmness of papaya tissues.
23670237	7	61	theme	polymers	1149:1156	arg1	mass					1115:1118	the molecular mass	1101:1118	the molecular mass of pectic and hemicellulosic polymers in frozen-thawed papayas using high-performance size-exclusion chromatography	1101:1234	This was due to a decrease in the molecular mass of pectic and hemicellulosic polymers in frozen-thawed papayas using high-performance size-exclusion chromatography.
23670237	8	62	theme	papaya	1406:1411	arg1	tissues					1413:1419	papaya tissues	1406:1419	papaya tissues	1406:1419	CONCLUSION The freezing and thawing processes caused fine structural damage and cell wall composition changes which contributed to a loss of drip volume and firmness of papaya tissues.
23670237	6	63	theme	significant	916:926	arg1	decrease					928:935	a significant decrease	914:935	a significant decrease in alcohol-insoluble solids, Na₂CO₃- and 24% KOH-soluble fractions	914:1002	These changes related with a significant decrease in alcohol-insoluble solids, Na₂CO₃- and 24% KOH-soluble fractions and an increase in the water-, EDTA- and 4% KOH-soluble fractions.
23670237	4	64	theme	materials	760:768	arg1	loss					742:745	loss	742:745	loss of cell wall materials in middle lamella	742:786	Light microscopy showed irregular shapes and cell damage in parenchyma cells of frozen-thawed papayas, whereas transmission electron microscopy showed loss of cell wall materials in middle lamella.
23670237	3	65	theme	freeze-thaw	571:581	arg1	cycles					583:588	freeze-thaw cycles	571:588	freeze-thaw cycles	571:588	RESULTS The frozen-thawed papayas had an increase in drip loss and a decrease in firmness with increasing number of freeze-thaw cycles.
23670237	8	66	theme	fine	1290:1293	arg1	damage					1306:1311	fine structural damage	1290:1311	fine structural damage	1290:1311	CONCLUSION The freezing and thawing processes caused fine structural damage and cell wall composition changes which contributed to a loss of drip volume and firmness of papaya tissues.
23670237	8	67	dep	CONCLUSION	1237:1246	arg1	caused					1283:1288	caused	1283:1288	caused fine structural damage and cell wall composition changes which contributed to a loss of drip volume and firmness of papaya tissues	1283:1419	CONCLUSION The freezing and thawing processes caused fine structural damage and cell wall composition changes which contributed to a loss of drip volume and firmness of papaya tissues.
23670237	0	68	from	changes	85:91	arg1	tissues					103:109	papaya tissues	96:109	papaya tissues	96:109	Effects of freezing and thawing on texture, microstructure and cell wall composition changes in papaya tissues.
23670237	3	69	theme	cycles	583:588	arg1	number					561:566	number	561:566	number of freeze-thaw cycles	561:588	RESULTS The frozen-thawed papayas had an increase in drip loss and a decrease in firmness with increasing number of freeze-thaw cycles.
23670237	2	70	from	texture	360:366	arg1	tissues					428:434	papaya tissues	421:434	papaya tissues	421:434	The effects of freezing and thawing on texture, microstructure and cell wall composition changes in papaya tissues were investigated.
23670237	4	71	theme	papayas	685:691	arg1	cells					662:666	parenchyma cells	651:666	parenchyma cells of frozen-thawed papayas	651:691	Light microscopy showed irregular shapes and cell damage in parenchyma cells of frozen-thawed papayas, whereas transmission electron microscopy showed loss of cell wall materials in middle lamella.
23670237	8	72	theme	cell	1317:1320	arg1	changes					1339:1345	cell wall composition changes	1317:1345	cell wall composition changes which contributed to a loss of drip volume and firmness of papaya tissues	1317:1419	CONCLUSION The freezing and thawing processes caused fine structural damage and cell wall composition changes which contributed to a loss of drip volume and firmness of papaya tissues.
23670237	4	73	theme	middle	773:778	arg1	lamella					780:786	middle lamella	773:786	middle lamella	773:786	Light microscopy showed irregular shapes and cell damage in parenchyma cells of frozen-thawed papayas, whereas transmission electron microscopy showed loss of cell wall materials in middle lamella.
23670237	3	74	from	decrease	524:531	arg1	firmness					536:543	firmness	536:543	firmness	536:543	RESULTS The frozen-thawed papayas had an increase in drip loss and a decrease in firmness with increasing number of freeze-thaw cycles.
23670237	3	74	from	decrease	524:531	arg1	loss					513:516	drip loss	508:516	drip loss	508:516	RESULTS The frozen-thawed papayas had an increase in drip loss and a decrease in firmness with increasing number of freeze-thaw cycles.
23670237	6	75	theme	KOH-soluble	982:992	arg1	fractions					994:1002	24% KOH-soluble fractions	978:1002	24% KOH-soluble fractions	978:1002	These changes related with a significant decrease in alcohol-insoluble solids, Na₂CO₃- and 24% KOH-soluble fractions and an increase in the water-, EDTA- and 4% KOH-soluble fractions.
23670237	7	76	theme	pectic	1123:1128	arg1	polymers					1149:1156	pectic and hemicellulosic polymers	1123:1156	polymers	1149:1156	This was due to a decrease in the molecular mass of pectic and hemicellulosic polymers in frozen-thawed papayas using high-performance size-exclusion chromatography.
23670237	7	77	theme	molecular	1105:1113	arg1	mass					1115:1118	the molecular mass	1101:1118	the molecular mass of pectic and hemicellulosic polymers in frozen-thawed papayas using high-performance size-exclusion chromatography	1101:1234	This was due to a decrease in the molecular mass of pectic and hemicellulosic polymers in frozen-thawed papayas using high-performance size-exclusion chromatography.
23670237	2	78	from	microstructure	369:382	arg1	tissues					428:434	papaya tissues	421:434	papaya tissues	421:434	The effects of freezing and thawing on texture, microstructure and cell wall composition changes in papaya tissues were investigated.
23670237	6	79	theme	KOH-soluble	1048:1058	arg1	fractions					1060:1068	the water-, EDTA- and 4% KOH-soluble fractions	1023:1068	the water-, EDTA- and 4% KOH-soluble fractions	1023:1068	These changes related with a significant decrease in alcohol-insoluble solids, Na₂CO₃- and 24% KOH-soluble fractions and an increase in the water-, EDTA- and 4% KOH-soluble fractions.
23670237	7	80	theme	high-performance	1189:1204	arg1	chromatography					1221:1234	high-performance size-exclusion chromatography	1189:1234	high-performance size-exclusion chromatography	1189:1234	This was due to a decrease in the molecular mass of pectic and hemicellulosic polymers in frozen-thawed papayas using high-performance size-exclusion chromatography.
23670237	6	81	theme	%	980:980	arg1	fractions					994:1002	24% KOH-soluble fractions	978:1002	24% KOH-soluble fractions	978:1002	These changes related with a significant decrease in alcohol-insoluble solids, Na₂CO₃- and 24% KOH-soluble fractions and an increase in the water-, EDTA- and 4% KOH-soluble fractions.
25753715	2	0	from	support	394:400	arg1	tools					431:435	software tools	422:435	software tools for 3D structure determination and validation	422:481	There are various reasons for this, one of which is a comparably low support of carbohydrates in software tools for 3D structure determination and validation.
25753715	1	1	theme	Data	147:150	arg1	PDB					158:160	PDB	158:160	PDB	158:160	The frequency of glycosylated protein 3D structures in the Protein Data Bank (PDB) is significantly lower than the proportion of glycoproteins in nature, and if glycan 3D structures are present, then they often exhibit a large degree of errors.
25753715	1	1	theme	Data	147:150	arg1	Bank					152:155	the Protein Data Bank	135:155	the Protein Data Bank (PDB)	135:161	The frequency of glycosylated protein 3D structures in the Protein Data Bank (PDB) is significantly lower than the proportion of glycoproteins in nature, and if glycan 3D structures are present, then they often exhibit a large degree of errors.
25753715	2	2	theme	carbohydrates	405:417	arg1	reasons					343:349	various reasons	335:349	various reasons	335:349	There are various reasons for this, one of which is a comparably low support of carbohydrates in software tools for 3D structure determination and validation.
25753715	2	2	theme	carbohydrates	405:417	arg1	support					394:400	a comparably low support	377:400	a comparably low support of carbohydrates in software tools for 3D structure determination and validation	377:481	There are various reasons for this, one of which is a comparably low support of carbohydrates in software tools for 3D structure determination and validation.
25753715	2	2	theme	carbohydrates	405:417	arg1	one					361:363	one	361:363	one	361:363	There are various reasons for this, one of which is a comparably low support of carbohydrates in software tools for 3D structure determination and validation.
25753715	3	3	theme	structure	592:600	arg1	determination					602:614	3D structure determination	589:614	3D structure determination in Coot and CNS	589:630	This chapter illustrates the current features that assist crystallographers with handling glycans during 3D structure determination in Coot and CNS and with validation of the results.
25753715	1	4	theme	large	301:305	arg1	degree					307:312	a large degree	299:312	a large degree of errors	299:322	The frequency of glycosylated protein 3D structures in the Protein Data Bank (PDB) is significantly lower than the proportion of glycoproteins in nature, and if glycan 3D structures are present, then they often exhibit a large degree of errors.
25753715	2	5	theme	structure	444:452	arg1	determination					454:466	3D structure determination	441:466	3D structure determination	441:466	There are various reasons for this, one of which is a comparably low support of carbohydrates in software tools for 3D structure determination and validation.
25753715	3	6	from	determination	602:614	arg1	CNS					628:630	CNS	628:630	CNS	628:630	This chapter illustrates the current features that assist crystallographers with handling glycans during 3D structure determination in Coot and CNS and with validation of the results.
25753715	3	6	from	determination	602:614	arg1	Coot					619:622	Coot	619:622	Coot	619:622	This chapter illustrates the current features that assist crystallographers with handling glycans during 3D structure determination in Coot and CNS and with validation of the results.
25753715	1	7	gly	glycosylated	97:108	arg1	structures					121:130	glycosylated protein 3D structures	97:130	glycosylated protein 3D structures	97:130	The frequency of glycosylated protein 3D structures in the Protein Data Bank (PDB) is significantly lower than the proportion of glycoproteins in nature, and if glycan 3D structures are present, then they often exhibit a large degree of errors.
25753715	1	8	gly	glycoproteins	209:221	arg1	glycoproteins					209:221	glycoproteins	209:221	glycoproteins	209:221	The frequency of glycosylated protein 3D structures in the Protein Data Bank (PDB) is significantly lower than the proportion of glycoproteins in nature, and if glycan 3D structures are present, then they often exhibit a large degree of errors.
25753715	2	9	theme	reasons	343:349	arg1	reasons					343:349	various reasons	335:349	various reasons	335:349	There are various reasons for this, one of which is a comparably low support of carbohydrates in software tools for 3D structure determination and validation.
25753715	2	9	theme	reasons	343:349	arg1	support					394:400	a comparably low support	377:400	a comparably low support of carbohydrates in software tools for 3D structure determination and validation	377:481	There are various reasons for this, one of which is a comparably low support of carbohydrates in software tools for 3D structure determination and validation.
25753715	2	9	theme	reasons	343:349	arg1	one					361:363	one	361:363	one	361:363	There are various reasons for this, one of which is a comparably low support of carbohydrates in software tools for 3D structure determination and validation.
25753715	3	10	with	crystallographers	542:558	arg1	glycans					574:580	handling glycans	565:580	handling glycans	565:580	This chapter illustrates the current features that assist crystallographers with handling glycans during 3D structure determination in Coot and CNS and with validation of the results.
25753715	1	11	theme	errors	317:322	arg1	degree					307:312	a large degree	299:312	a large degree of errors	299:322	The frequency of glycosylated protein 3D structures in the Protein Data Bank (PDB) is significantly lower than the proportion of glycoproteins in nature, and if glycan 3D structures are present, then they often exhibit a large degree of errors.
25753715	2	12	theme	various	335:341	arg1	reasons					343:349	various reasons	335:349	various reasons	335:349	There are various reasons for this, one of which is a comparably low support of carbohydrates in software tools for 3D structure determination and validation.
25753715	0	13	theme	data	74:77	arg1	validation					34:43	validation	34:43	validation	34:43	Tools to assist determination and validation of carbohydrate 3D structure data.
25753715	0	13	theme	data	74:77	arg1	determination					16:28	determination	16:28	determination	16:28	Tools to assist determination and validation of carbohydrate 3D structure data.
25753715	1	14	theme	glycan	241:246	arg1	structures					251:260	glycan 3D structures	241:260	glycan 3D structures	241:260	The frequency of glycosylated protein 3D structures in the Protein Data Bank (PDB) is significantly lower than the proportion of glycoproteins in nature, and if glycan 3D structures are present, then they often exhibit a large degree of errors.
25753715	3	15	theme	current	513:519	arg1	features					521:528	the current features	509:528	the current features that assist crystallographers with handling glycans during 3D structure determination in Coot and CNS and with validation of the results	509:665	This chapter illustrates the current features that assist crystallographers with handling glycans during 3D structure determination in Coot and CNS and with validation of the results.
25753715	1	16	theme	3D	248:249	arg1	structures					251:260	glycan 3D structures	241:260	glycan 3D structures	241:260	The frequency of glycosylated protein 3D structures in the Protein Data Bank (PDB) is significantly lower than the proportion of glycoproteins in nature, and if glycan 3D structures are present, then they often exhibit a large degree of errors.
25753715	1	17	theme	glycosylated	97:108	arg1	structures					121:130	glycosylated protein 3D structures	97:130	glycosylated protein 3D structures	97:130	The frequency of glycosylated protein 3D structures in the Protein Data Bank (PDB) is significantly lower than the proportion of glycoproteins in nature, and if glycan 3D structures are present, then they often exhibit a large degree of errors.
25753715	2	18	theme	low	390:392	arg1	reasons					343:349	various reasons	335:349	various reasons	335:349	There are various reasons for this, one of which is a comparably low support of carbohydrates in software tools for 3D structure determination and validation.
25753715	2	18	theme	low	390:392	arg1	support					394:400	a comparably low support	377:400	a comparably low support of carbohydrates in software tools for 3D structure determination and validation	377:481	There are various reasons for this, one of which is a comparably low support of carbohydrates in software tools for 3D structure determination and validation.
25753715	2	18	theme	low	390:392	arg1	one					361:363	one	361:363	one	361:363	There are various reasons for this, one of which is a comparably low support of carbohydrates in software tools for 3D structure determination and validation.
25753715	2	19	from	carbohydrates	405:417	arg1	tools					431:435	software tools	422:435	software tools for 3D structure determination and validation	422:481	There are various reasons for this, one of which is a comparably low support of carbohydrates in software tools for 3D structure determination and validation.
25753715	3	20	theme	3D	589:590	arg1	determination					602:614	3D structure determination	589:614	3D structure determination in Coot and CNS	589:630	This chapter illustrates the current features that assist crystallographers with handling glycans during 3D structure determination in Coot and CNS and with validation of the results.
25753715	2	21	from	tools	431:435	arg1	reasons					343:349	various reasons	335:349	various reasons	335:349	There are various reasons for this, one of which is a comparably low support of carbohydrates in software tools for 3D structure determination and validation.
25753715	2	21	from	tools	431:435	arg1	support					394:400	a comparably low support	377:400	a comparably low support of carbohydrates in software tools for 3D structure determination and validation	377:481	There are various reasons for this, one of which is a comparably low support of carbohydrates in software tools for 3D structure determination and validation.
25753715	2	21	from	tools	431:435	arg1	one					361:363	one	361:363	one	361:363	There are various reasons for this, one of which is a comparably low support of carbohydrates in software tools for 3D structure determination and validation.
25753715	0	22	theme	carbohydrate	48:59	arg1	data					74:77	carbohydrate 3D structure data	48:77	carbohydrate 3D structure data	48:77	Tools to assist determination and validation of carbohydrate 3D structure data.
25753715	2	23	theme	3D	441:442	arg1	determination					454:466	3D structure determination	441:466	3D structure determination	441:466	There are various reasons for this, one of which is a comparably low support of carbohydrates in software tools for 3D structure determination and validation.
25753715	0	24	theme	3D	61:62	arg1	data					74:77	carbohydrate 3D structure data	48:77	carbohydrate 3D structure data	48:77	Tools to assist determination and validation of carbohydrate 3D structure data.
25753715	3	25	theme	results	659:665	arg1	validation					641:650	validation	641:650	validation of the results	641:665	This chapter illustrates the current features that assist crystallographers with handling glycans during 3D structure determination in Coot and CNS and with validation of the results.
25753715	1	26	theme	protein	110:116	arg1	structures					121:130	glycosylated protein 3D structures	97:130	glycosylated protein 3D structures	97:130	The frequency of glycosylated protein 3D structures in the Protein Data Bank (PDB) is significantly lower than the proportion of glycoproteins in nature, and if glycan 3D structures are present, then they often exhibit a large degree of errors.
25753715	1	27	theme	3D	118:119	arg1	structures					121:130	glycosylated protein 3D structures	97:130	glycosylated protein 3D structures	97:130	The frequency of glycosylated protein 3D structures in the Protein Data Bank (PDB) is significantly lower than the proportion of glycoproteins in nature, and if glycan 3D structures are present, then they often exhibit a large degree of errors.
25753715	1	28	theme	structures	121:130	arg1	frequency					84:92	The frequency	80:92	The frequency of glycosylated protein 3D structures in the Protein Data Bank (PDB)	80:161	The frequency of glycosylated protein 3D structures in the Protein Data Bank (PDB) is significantly lower than the proportion of glycoproteins in nature, and if glycan 3D structures are present, then they often exhibit a large degree of errors.
25753715	1	28	theme	structures	121:130	arg1	lower					180:184	lower	180:184	lower	180:184	The frequency of glycosylated protein 3D structures in the Protein Data Bank (PDB) is significantly lower than the proportion of glycoproteins in nature, and if glycan 3D structures are present, then they often exhibit a large degree of errors.
25753715	0	29	theme	structure	64:72	arg1	data					74:77	carbohydrate 3D structure data	48:77	carbohydrate 3D structure data	48:77	Tools to assist determination and validation of carbohydrate 3D structure data.
25753715	1	30	from	frequency	84:92	arg1	PDB					158:160	PDB	158:160	PDB	158:160	The frequency of glycosylated protein 3D structures in the Protein Data Bank (PDB) is significantly lower than the proportion of glycoproteins in nature, and if glycan 3D structures are present, then they often exhibit a large degree of errors.
25753715	1	30	from	frequency	84:92	arg1	Bank					152:155	the Protein Data Bank	135:155	the Protein Data Bank (PDB)	135:161	The frequency of glycosylated protein 3D structures in the Protein Data Bank (PDB) is significantly lower than the proportion of glycoproteins in nature, and if glycan 3D structures are present, then they often exhibit a large degree of errors.
25753715	1	31	theme	glycoproteins	209:221	arg1	proportion					195:204	the proportion	191:204	the proportion of glycoproteins	191:221	The frequency of glycosylated protein 3D structures in the Protein Data Bank (PDB) is significantly lower than the proportion of glycoproteins in nature, and if glycan 3D structures are present, then they often exhibit a large degree of errors.
25753715	2	32	theme	software	422:429	arg1	tools					431:435	software tools	422:435	software tools for 3D structure determination and validation	422:481	There are various reasons for this, one of which is a comparably low support of carbohydrates in software tools for 3D structure determination and validation.
25753715	3	33	theme	handling	565:572	arg1	glycans					574:580	handling glycans	565:580	handling glycans	565:580	This chapter illustrates the current features that assist crystallographers with handling glycans during 3D structure determination in Coot and CNS and with validation of the results.
25753715	1	34	theme	Protein	139:145	arg1	PDB					158:160	PDB	158:160	PDB	158:160	The frequency of glycosylated protein 3D structures in the Protein Data Bank (PDB) is significantly lower than the proportion of glycoproteins in nature, and if glycan 3D structures are present, then they often exhibit a large degree of errors.
25753715	1	34	theme	Protein	139:145	arg1	Bank					152:155	the Protein Data Bank	135:155	the Protein Data Bank (PDB)	135:161	The frequency of glycosylated protein 3D structures in the Protein Data Bank (PDB) is significantly lower than the proportion of glycoproteins in nature, and if glycan 3D structures are present, then they often exhibit a large degree of errors.
28620376	7	0	theme	mannose	1393:1399	arg1	structures					1401:1410	high mannose structures	1388:1410	high mannose structures	1388:1410	In addition, we report glycosylation features not previously reported in an Fc-specific way on murine IgG, including monoantennary, hybrid, and high mannose structures, as well as diantennary structures without a core fucose, with a bisecting N-acetylglucosamine, or with α1,3-galactosylation.
28620376	8	1	theme	Pronounced	1538:1547	arg1	differences					1549:1559	Pronounced differences	1538:1559	Pronounced differences	1538:1559	Pronounced differences were detected between strains and the IgG subclasses within each strain.
28620376	4	2	gly	Fc-glycosylation	593:608	arg1	disease					664:670	disease	664:670	disease	664:670	However, while human IgG Fc-glycosylation has been extensively characterized in both health and disease, this is not the case for mice.
28620376	4	2	gly	Fc-glycosylation	593:608	arg1	health					653:658	health	653:658	health	653:658	However, while human IgG Fc-glycosylation has been extensively characterized in both health and disease, this is not the case for mice.
28620376	3	3	from	effects	442:448	arg1	functions					486:494	IgG effector functions	473:494	IgG effector functions	473:494	The mouse is often used as experimental animal model to study the effects of Fc-glycosylation on IgG effector functions, and results are not uncommonly translated back to the human situation.
28620376	3	4	theme	effector	477:484	arg1	functions					486:494	IgG effector functions	473:494	IgG effector functions	473:494	The mouse is often used as experimental animal model to study the effects of Fc-glycosylation on IgG effector functions, and results are not uncommonly translated back to the human situation.
28620376	1	5	theme	large	231:235	arg1	influence					237:245	a large influence	229:245	a large influence	229:245	N-linked glycosylation of the fragment crystallizable (Fc)-region of immunoglobulin G (IgG) is known to have a large influence on the activity of the antibody, an effect reported to be IgG subclass specific.
28620376	4	6	theme	human	583:587	arg1	Fc-glycosylation					593:608	human IgG Fc-glycosylation	583:608	human IgG Fc-glycosylation	583:608	However, while human IgG Fc-glycosylation has been extensively characterized in both health and disease, this is not the case for mice.
28620376	1	7	theme	Fc	175:176	arg1	-region					178:184	the fragment crystallizable (Fc)-region	146:184	the fragment crystallizable (Fc)-region of immunoglobulin G (IgG)	146:210	N-linked glycosylation of the fragment crystallizable (Fc)-region of immunoglobulin G (IgG) is known to have a large influence on the activity of the antibody, an effect reported to be IgG subclass specific.
28620376	7	8	theme	Fc-specific	1320:1330	arg1	way					1332:1334	an Fc-specific way	1317:1334	an Fc-specific way on murine IgG, including monoantennary, hybrid, and high mannose structures	1317:1410	In addition, we report glycosylation features not previously reported in an Fc-specific way on murine IgG, including monoantennary, hybrid, and high mannose structures, as well as diantennary structures without a core fucose, with a bisecting N-acetylglucosamine, or with α1,3-galactosylation.
28620376	10	9	theme	mouse	1953:1957	arg1	studies					1959:1965	immunological and glycobiological mouse studies	1919:1965	immunological and glycobiological mouse studies involving IgG effector functions	1919:1998	Mouse strain-based and subclass-specific glycosylation differences should be taken into account when designing and interpreting immunological and glycobiological mouse studies involving IgG effector functions.
28620376	1	10	theme	-region	178:184	arg1	glycosylation					129:141	N-linked glycosylation	120:141	N-linked glycosylation of the fragment crystallizable (Fc)-region of immunoglobulin G (IgG)	120:210	N-linked glycosylation of the fragment crystallizable (Fc)-region of immunoglobulin G (IgG) is known to have a large influence on the activity of the antibody, an effect reported to be IgG subclass specific.
28620376	5	11	theme	subclass-specific	963:979	arg1	Fc-glycopeptides					985:1000	the subclass-specific IgG Fc-glycopeptides	959:1000	the subclass-specific IgG Fc-glycopeptides of male and female mice from the strains BALB/c, C57BL/6, CD-1, and Swiss Webster	959:1082	To characterize the glycosylation profile of murine IgG-Fc and in addition evaluate the systematic glycosylation differences between mouse strains, sexes, and IgG subclasses, we used nanoliquid chromatography mass spectrometry (nanoLC-MS(/MS)) to look at the subclass-specific IgG Fc-glycopeptides of male and female mice from the strains BALB/c, C57BL/6, CD-1, and Swiss Webster.
28620376	7	12	with	structures	1436:1445	arg1	α1,3-galactosylation					1516:1535	α1,3-galactosylation	1516:1535	α1,3-galactosylation	1516:1535	In addition, we report glycosylation features not previously reported in an Fc-specific way on murine IgG, including monoantennary, hybrid, and high mannose structures, as well as diantennary structures without a core fucose, with a bisecting N-acetylglucosamine, or with α1,3-galactosylation.
28620376	7	12	with	structures	1436:1445	arg1	N-acetylglucosamine					1487:1505	a bisecting N-acetylglucosamine	1475:1505	a bisecting N-acetylglucosamine	1475:1505	In addition, we report glycosylation features not previously reported in an Fc-specific way on murine IgG, including monoantennary, hybrid, and high mannose structures, as well as diantennary structures without a core fucose, with a bisecting N-acetylglucosamine, or with α1,3-galactosylation.
28620376	5	13	theme	IgG-Fc	756:761	arg1	profile					738:744	the glycosylation profile	720:744	the glycosylation profile of murine IgG-Fc and in addition	720:777	To characterize the glycosylation profile of murine IgG-Fc and in addition evaluate the systematic glycosylation differences between mouse strains, sexes, and IgG subclasses, we used nanoliquid chromatography mass spectrometry (nanoLC-MS(/MS)) to look at the subclass-specific IgG Fc-glycopeptides of male and female mice from the strains BALB/c, C57BL/6, CD-1, and Swiss Webster.
28620376	10	14	theme	effector	1981:1988	arg1	functions					1990:1998	IgG effector functions	1977:1998	IgG effector functions	1977:1998	Mouse strain-based and subclass-specific glycosylation differences should be taken into account when designing and interpreting immunological and glycobiological mouse studies involving IgG effector functions.
28620376	6	15	theme	α2,6-sialylation	1226:1241	arg1	galactosylation					1206:1220	galactosylation	1206:1220	galactosylation	1206:1220	The structural analysis revealed the presence of predominantly fucosylated, diantennary glycans, with varying amounts of galactosylation and α2,6-sialylation.
28620376	6	15	theme	α2,6-sialylation	1226:1241	arg1	amounts					1195:1201	varying amounts	1187:1201	varying amounts of galactosylation and α2,6-sialylation	1187:1241	The structural analysis revealed the presence of predominantly fucosylated, diantennary glycans, with varying amounts of galactosylation and α2,6-sialylation.
28620376	6	15	theme	α2,6-sialylation	1226:1241	arg1	α2,6-sialylation					1226:1241	α2,6-sialylation	1226:1241	α2,6-sialylation	1226:1241	The structural analysis revealed the presence of predominantly fucosylated, diantennary glycans, with varying amounts of galactosylation and α2,6-sialylation.
28620376	6	16	theme	galactosylation	1206:1220	arg1	galactosylation					1206:1220	galactosylation	1206:1220	galactosylation	1206:1220	The structural analysis revealed the presence of predominantly fucosylated, diantennary glycans, with varying amounts of galactosylation and α2,6-sialylation.
28620376	6	16	theme	galactosylation	1206:1220	arg1	amounts					1195:1201	varying amounts	1187:1201	varying amounts of galactosylation and α2,6-sialylation	1187:1241	The structural analysis revealed the presence of predominantly fucosylated, diantennary glycans, with varying amounts of galactosylation and α2,6-sialylation.
28620376	6	16	theme	galactosylation	1206:1220	arg1	α2,6-sialylation					1226:1241	α2,6-sialylation	1226:1241	α2,6-sialylation	1226:1241	The structural analysis revealed the presence of predominantly fucosylated, diantennary glycans, with varying amounts of galactosylation and α2,6-sialylation.
28620376	3	17	theme	human	551:555	arg1	situation					557:565	the human situation	547:565	the human situation	547:565	The mouse is often used as experimental animal model to study the effects of Fc-glycosylation on IgG effector functions, and results are not uncommonly translated back to the human situation.
28620376	6	18	gly	fucosylated	1148:1158	arg1	glycans					1173:1179	predominantly fucosylated, diantennary glycans	1134:1179	predominantly fucosylated, diantennary glycans	1134:1179	The structural analysis revealed the presence of predominantly fucosylated, diantennary glycans, with varying amounts of galactosylation and α2,6-sialylation.
28620376	9	19	theme	galactosylation	1665:1679	arg1	levels					1697:1702	galactosylation and sialylation levels	1665:1702	galactosylation and sialylation levels found between both strains and subclasses	1665:1744	Especially the large spread in galactosylation and sialylation levels found between both strains and subclasses may vastly influence IgG effector functions.
28620376	9	20	from	spread	1655:1660	arg1	levels					1697:1702	galactosylation and sialylation levels	1665:1702	galactosylation and sialylation levels found between both strains and subclasses	1665:1744	Especially the large spread in galactosylation and sialylation levels found between both strains and subclasses may vastly influence IgG effector functions.
28620376	9	21	theme	sialylation	1685:1695	arg1	levels					1697:1702	galactosylation and sialylation levels	1665:1702	galactosylation and sialylation levels found between both strains and subclasses	1665:1744	Especially the large spread in galactosylation and sialylation levels found between both strains and subclasses may vastly influence IgG effector functions.
28620376	0	22	theme	Crystallizable	61:74	arg1	G					44:44	Mouse Immunoglobulin G	23:44	Mouse Immunoglobulin G (IgG)-Fragment Crystallizable	23:74	The N-Glycosylation of Mouse Immunoglobulin G (IgG)-Fragment Crystallizable Differs Between IgG Subclasses and Strains.
28620376	0	22	theme	Crystallizable	61:74	arg1	IgG					47:49	IgG	47:49	IgG	47:49	The N-Glycosylation of Mouse Immunoglobulin G (IgG)-Fragment Crystallizable Differs Between IgG Subclasses and Strains.
28620376	5	23	theme	mouse	837:841	arg1	strains					843:849	mouse strains	837:849	mouse strains	837:849	To characterize the glycosylation profile of murine IgG-Fc and in addition evaluate the systematic glycosylation differences between mouse strains, sexes, and IgG subclasses, we used nanoliquid chromatography mass spectrometry (nanoLC-MS(/MS)) to look at the subclass-specific IgG Fc-glycopeptides of male and female mice from the strains BALB/c, C57BL/6, CD-1, and Swiss Webster.
28620376	7	24	theme	glycosylation	1267:1279	arg1	features					1281:1288	glycosylation features	1267:1288	glycosylation features not previously reported in an Fc-specific way on murine IgG, including monoantennary, hybrid, and high mannose structures	1267:1410	In addition, we report glycosylation features not previously reported in an Fc-specific way on murine IgG, including monoantennary, hybrid, and high mannose structures, as well as diantennary structures without a core fucose, with a bisecting N-acetylglucosamine, or with α1,3-galactosylation.
28620376	5	25	from	profile	738:744	arg1	addition					770:777	addition	770:777	addition	770:777	To characterize the glycosylation profile of murine IgG-Fc and in addition evaluate the systematic glycosylation differences between mouse strains, sexes, and IgG subclasses, we used nanoliquid chromatography mass spectrometry (nanoLC-MS(/MS)) to look at the subclass-specific IgG Fc-glycopeptides of male and female mice from the strains BALB/c, C57BL/6, CD-1, and Swiss Webster.
28620376	7	26	theme	core	1457:1460	arg1	fucose					1462:1467	a core fucose	1455:1467	a core fucose	1455:1467	In addition, we report glycosylation features not previously reported in an Fc-specific way on murine IgG, including monoantennary, hybrid, and high mannose structures, as well as diantennary structures without a core fucose, with a bisecting N-acetylglucosamine, or with α1,3-galactosylation.
28620376	5	27	theme	female	1014:1019	arg1	mice					1021:1024	male and female mice	1005:1024	male and female mice	1005:1024	To characterize the glycosylation profile of murine IgG-Fc and in addition evaluate the systematic glycosylation differences between mouse strains, sexes, and IgG subclasses, we used nanoliquid chromatography mass spectrometry (nanoLC-MS(/MS)) to look at the subclass-specific IgG Fc-glycopeptides of male and female mice from the strains BALB/c, C57BL/6, CD-1, and Swiss Webster.
28620376	0	28	theme	G	44:44	arg1	N-Glycosylation					4:18	The N-Glycosylation	0:18	The N-Glycosylation of Mouse Immunoglobulin G (IgG)-Fragment Crystallizable	0:74	The N-Glycosylation of Mouse Immunoglobulin G (IgG)-Fragment Crystallizable Differs Between IgG Subclasses and Strains.
28620376	5	29	theme	chromatography	898:911	arg1	spectrometry					918:929	nanoliquid chromatography mass spectrometry	887:929	nanoliquid chromatography mass spectrometry (nanoLC-MS(/MS))	887:946	To characterize the glycosylation profile of murine IgG-Fc and in addition evaluate the systematic glycosylation differences between mouse strains, sexes, and IgG subclasses, we used nanoliquid chromatography mass spectrometry (nanoLC-MS(/MS)) to look at the subclass-specific IgG Fc-glycopeptides of male and female mice from the strains BALB/c, C57BL/6, CD-1, and Swiss Webster.
28620376	5	30	theme	glycosylation	724:736	arg1	profile					738:744	the glycosylation profile	720:744	the glycosylation profile of murine IgG-Fc and in addition	720:777	To characterize the glycosylation profile of murine IgG-Fc and in addition evaluate the systematic glycosylation differences between mouse strains, sexes, and IgG subclasses, we used nanoliquid chromatography mass spectrometry (nanoLC-MS(/MS)) to look at the subclass-specific IgG Fc-glycopeptides of male and female mice from the strains BALB/c, C57BL/6, CD-1, and Swiss Webster.
28620376	5	31	theme	IgG	863:865	arg1	subclasses					867:876	IgG subclasses	863:876	IgG subclasses	863:876	To characterize the glycosylation profile of murine IgG-Fc and in addition evaluate the systematic glycosylation differences between mouse strains, sexes, and IgG subclasses, we used nanoliquid chromatography mass spectrometry (nanoLC-MS(/MS)) to look at the subclass-specific IgG Fc-glycopeptides of male and female mice from the strains BALB/c, C57BL/6, CD-1, and Swiss Webster.
28620376	5	32	gly	glycosylation	724:736	arg1	IgG-Fc					756:761	murine IgG-Fc	749:761	murine IgG-Fc	749:761	To characterize the glycosylation profile of murine IgG-Fc and in addition evaluate the systematic glycosylation differences between mouse strains, sexes, and IgG subclasses, we used nanoliquid chromatography mass spectrometry (nanoLC-MS(/MS)) to look at the subclass-specific IgG Fc-glycopeptides of male and female mice from the strains BALB/c, C57BL/6, CD-1, and Swiss Webster.
28620376	5	33	used	used	882:885	arg2	we					879:880	we	879:880	we	879:880	To characterize the glycosylation profile of murine IgG-Fc and in addition evaluate the systematic glycosylation differences between mouse strains, sexes, and IgG subclasses, we used nanoliquid chromatography mass spectrometry (nanoLC-MS(/MS)) to look at the subclass-specific IgG Fc-glycopeptides of male and female mice from the strains BALB/c, C57BL/6, CD-1, and Swiss Webster.
28620376	0	34	theme	Immunoglobulin	29:42	arg1	G					44:44	Mouse Immunoglobulin G	23:44	Mouse Immunoglobulin G (IgG)-Fragment Crystallizable	23:74	The N-Glycosylation of Mouse Immunoglobulin G (IgG)-Fragment Crystallizable Differs Between IgG Subclasses and Strains.
28620376	0	34	theme	Immunoglobulin	29:42	arg1	IgG					47:49	IgG	47:49	IgG	47:49	The N-Glycosylation of Mouse Immunoglobulin G (IgG)-Fragment Crystallizable Differs Between IgG Subclasses and Strains.
28620376	0	35	gly	N-Glycosylation	4:18	arg1	G					44:44	Mouse Immunoglobulin G	23:44	Mouse Immunoglobulin G (IgG)-Fragment Crystallizable	23:74	The N-Glycosylation of Mouse Immunoglobulin G (IgG)-Fragment Crystallizable Differs Between IgG Subclasses and Strains.
28620376	0	35	gly	N-Glycosylation	4:18	arg1	IgG					47:49	IgG	47:49	IgG	47:49	The N-Glycosylation of Mouse Immunoglobulin G (IgG)-Fragment Crystallizable Differs Between IgG Subclasses and Strains.
28620376	9	36	theme	effector	1771:1778	arg1	functions					1780:1788	IgG effector functions	1767:1788	IgG effector functions	1767:1788	Especially the large spread in galactosylation and sialylation levels found between both strains and subclasses may vastly influence IgG effector functions.
28620376	1	37	link	N-linked	120:127	arg1	glycosylation					129:141	N-linked glycosylation	120:141	N-linked glycosylation of the fragment crystallizable (Fc)-region of immunoglobulin G (IgG)	120:210	N-linked glycosylation of the fragment crystallizable (Fc)-region of immunoglobulin G (IgG) is known to have a large influence on the activity of the antibody, an effect reported to be IgG subclass specific.
28620376	1	38	theme	antibody	270:277	arg1	activity					254:261	the activity	250:261	the activity of the antibody	250:277	N-linked glycosylation of the fragment crystallizable (Fc)-region of immunoglobulin G (IgG) is known to have a large influence on the activity of the antibody, an effect reported to be IgG subclass specific.
28620376	1	38	theme	antibody	270:277	arg1	effect					283:288	an effect	280:288	an effect reported to be IgG subclass specific	280:325	N-linked glycosylation of the fragment crystallizable (Fc)-region of immunoglobulin G (IgG) is known to have a large influence on the activity of the antibody, an effect reported to be IgG subclass specific.
28620376	3	39	theme	experimental	403:414	arg1	model					423:427	experimental animal model	403:427	experimental animal model	403:427	The mouse is often used as experimental animal model to study the effects of Fc-glycosylation on IgG effector functions, and results are not uncommonly translated back to the human situation.
28620376	3	39	theme	experimental	403:414	arg1	mouse					380:384	The mouse	376:384	The mouse	376:384	The mouse is often used as experimental animal model to study the effects of Fc-glycosylation on IgG effector functions, and results are not uncommonly translated back to the human situation.
28620376	10	40	theme	subclass-specific	1814:1830	arg1	differences					1846:1856	Mouse strain-based and subclass-specific glycosylation differences	1791:1856	differences	1846:1856	Mouse strain-based and subclass-specific glycosylation differences should be taken into account when designing and interpreting immunological and glycobiological mouse studies involving IgG effector functions.
28620376	7	41	theme	high	1388:1391	arg1	structures					1401:1410	high mannose structures	1388:1410	high mannose structures	1388:1410	In addition, we report glycosylation features not previously reported in an Fc-specific way on murine IgG, including monoantennary, hybrid, and high mannose structures, as well as diantennary structures without a core fucose, with a bisecting N-acetylglucosamine, or with α1,3-galactosylation.
28620376	6	42	theme	varying	1187:1193	arg1	galactosylation					1206:1220	galactosylation	1206:1220	galactosylation	1206:1220	The structural analysis revealed the presence of predominantly fucosylated, diantennary glycans, with varying amounts of galactosylation and α2,6-sialylation.
28620376	6	42	theme	varying	1187:1193	arg1	amounts					1195:1201	varying amounts	1187:1201	varying amounts of galactosylation and α2,6-sialylation	1187:1241	The structural analysis revealed the presence of predominantly fucosylated, diantennary glycans, with varying amounts of galactosylation and α2,6-sialylation.
28620376	6	42	theme	varying	1187:1193	arg1	α2,6-sialylation					1226:1241	α2,6-sialylation	1226:1241	α2,6-sialylation	1226:1241	The structural analysis revealed the presence of predominantly fucosylated, diantennary glycans, with varying amounts of galactosylation and α2,6-sialylation.
28620376	3	43	theme	IgG	473:475	arg1	functions					486:494	IgG effector functions	473:494	IgG effector functions	473:494	The mouse is often used as experimental animal model to study the effects of Fc-glycosylation on IgG effector functions, and results are not uncommonly translated back to the human situation.
28620376	6	44	theme	glycans	1173:1179	arg1	presence					1122:1129	the presence	1118:1129	the presence of predominantly fucosylated, diantennary glycans	1118:1179	The structural analysis revealed the presence of predominantly fucosylated, diantennary glycans, with varying amounts of galactosylation and α2,6-sialylation.
28620376	3	45	theme	Fc-glycosylation	453:468	arg1	effects					442:448	the effects	438:448	the effects of Fc-glycosylation on IgG effector functions	438:494	The mouse is often used as experimental animal model to study the effects of Fc-glycosylation on IgG effector functions, and results are not uncommonly translated back to the human situation.
28620376	5	46	dep	characterize	707:718	arg1	evaluate					779:786	evaluate	779:786	evaluate the systematic glycosylation differences between mouse strains, sexes, and IgG subclasses	779:876	To characterize the glycosylation profile of murine IgG-Fc and in addition evaluate the systematic glycosylation differences between mouse strains, sexes, and IgG subclasses, we used nanoliquid chromatography mass spectrometry (nanoLC-MS(/MS)) to look at the subclass-specific IgG Fc-glycopeptides of male and female mice from the strains BALB/c, C57BL/6, CD-1, and Swiss Webster.
28620376	7	47	theme	murine	1339:1344	arg1	IgG					1346:1348	murine IgG	1339:1348	murine IgG	1339:1348	In addition, we report glycosylation features not previously reported in an Fc-specific way on murine IgG, including monoantennary, hybrid, and high mannose structures, as well as diantennary structures without a core fucose, with a bisecting N-acetylglucosamine, or with α1,3-galactosylation.
28620376	6	48	theme	fucosylated	1148:1158	arg1	glycans					1173:1179	predominantly fucosylated, diantennary glycans	1134:1179	predominantly fucosylated, diantennary glycans	1134:1179	The structural analysis revealed the presence of predominantly fucosylated, diantennary glycans, with varying amounts of galactosylation and α2,6-sialylation.
28620376	4	49	theme	IgG	589:591	arg1	Fc-glycosylation					593:608	human IgG Fc-glycosylation	583:608	human IgG Fc-glycosylation	583:608	However, while human IgG Fc-glycosylation has been extensively characterized in both health and disease, this is not the case for mice.
28620376	10	50	theme	glycobiological	1937:1951	arg1	studies					1959:1965	immunological and glycobiological mouse studies	1919:1965	immunological and glycobiological mouse studies involving IgG effector functions	1919:1998	Mouse strain-based and subclass-specific glycosylation differences should be taken into account when designing and interpreting immunological and glycobiological mouse studies involving IgG effector functions.
28620376	5	51	theme	murine	749:754	arg1	IgG-Fc					756:761	murine IgG-Fc	749:761	murine IgG-Fc	749:761	To characterize the glycosylation profile of murine IgG-Fc and in addition evaluate the systematic glycosylation differences between mouse strains, sexes, and IgG subclasses, we used nanoliquid chromatography mass spectrometry (nanoLC-MS(/MS)) to look at the subclass-specific IgG Fc-glycopeptides of male and female mice from the strains BALB/c, C57BL/6, CD-1, and Swiss Webster.
28620376	1	52	theme	crystallizable	159:172	arg1	-region					178:184	the fragment crystallizable (Fc)-region	146:184	the fragment crystallizable (Fc)-region of immunoglobulin G (IgG)	146:210	N-linked glycosylation of the fragment crystallizable (Fc)-region of immunoglobulin G (IgG) is known to have a large influence on the activity of the antibody, an effect reported to be IgG subclass specific.
28620376	10	53	theme	immunological	1919:1931	arg1	studies					1959:1965	immunological and glycobiological mouse studies	1919:1965	immunological and glycobiological mouse studies involving IgG effector functions	1919:1998	Mouse strain-based and subclass-specific glycosylation differences should be taken into account when designing and interpreting immunological and glycobiological mouse studies involving IgG effector functions.
28620376	1	54	gly	glycosylation	129:141	arg1	-region					178:184	the fragment crystallizable (Fc)-region	146:184	the fragment crystallizable (Fc)-region of immunoglobulin G (IgG)	146:210	N-linked glycosylation of the fragment crystallizable (Fc)-region of immunoglobulin G (IgG) is known to have a large influence on the activity of the antibody, an effect reported to be IgG subclass specific.
28620376	5	55	theme	IgG	981:983	arg1	Fc-glycopeptides					985:1000	the subclass-specific IgG Fc-glycopeptides	959:1000	the subclass-specific IgG Fc-glycopeptides of male and female mice from the strains BALB/c, C57BL/6, CD-1, and Swiss Webster	959:1082	To characterize the glycosylation profile of murine IgG-Fc and in addition evaluate the systematic glycosylation differences between mouse strains, sexes, and IgG subclasses, we used nanoliquid chromatography mass spectrometry (nanoLC-MS(/MS)) to look at the subclass-specific IgG Fc-glycopeptides of male and female mice from the strains BALB/c, C57BL/6, CD-1, and Swiss Webster.
28620376	5	56	theme	systematic	792:801	arg1	differences					817:827	the systematic glycosylation differences	788:827	the systematic glycosylation differences between mouse strains, sexes, and IgG subclasses	788:876	To characterize the glycosylation profile of murine IgG-Fc and in addition evaluate the systematic glycosylation differences between mouse strains, sexes, and IgG subclasses, we used nanoliquid chromatography mass spectrometry (nanoLC-MS(/MS)) to look at the subclass-specific IgG Fc-glycopeptides of male and female mice from the strains BALB/c, C57BL/6, CD-1, and Swiss Webster.
28620376	1	57	theme	G	204:204	arg1	-region					178:184	the fragment crystallizable (Fc)-region	146:184	the fragment crystallizable (Fc)-region of immunoglobulin G (IgG)	146:210	N-linked glycosylation of the fragment crystallizable (Fc)-region of immunoglobulin G (IgG) is known to have a large influence on the activity of the antibody, an effect reported to be IgG subclass specific.
28620376	5	58	dep	spectrometry	918:929	arg1	/MS					942:944	/MS	942:944	/MS	942:944	To characterize the glycosylation profile of murine IgG-Fc and in addition evaluate the systematic glycosylation differences between mouse strains, sexes, and IgG subclasses, we used nanoliquid chromatography mass spectrometry (nanoLC-MS(/MS)) to look at the subclass-specific IgG Fc-glycopeptides of male and female mice from the strains BALB/c, C57BL/6, CD-1, and Swiss Webster.
28620376	5	58	dep	spectrometry	918:929	arg1	nanoLC-MS					932:940	nanoLC-MS	932:940	nanoLC-MS(/MS)	932:945	To characterize the glycosylation profile of murine IgG-Fc and in addition evaluate the systematic glycosylation differences between mouse strains, sexes, and IgG subclasses, we used nanoliquid chromatography mass spectrometry (nanoLC-MS(/MS)) to look at the subclass-specific IgG Fc-glycopeptides of male and female mice from the strains BALB/c, C57BL/6, CD-1, and Swiss Webster.
28620376	5	59	from	Fc-glycopeptides	985:1000	arg1	look					951:954	look	951:954	look at the subclass-specific IgG Fc-glycopeptides of male and female mice from the strains BALB/c, C57BL/6, CD-1, and Swiss Webster	951:1082	To characterize the glycosylation profile of murine IgG-Fc and in addition evaluate the systematic glycosylation differences between mouse strains, sexes, and IgG subclasses, we used nanoliquid chromatography mass spectrometry (nanoLC-MS(/MS)) to look at the subclass-specific IgG Fc-glycopeptides of male and female mice from the strains BALB/c, C57BL/6, CD-1, and Swiss Webster.
28620376	9	60	theme	large	1649:1653	arg1	spread					1655:1660	Especially the large spread	1634:1660	Especially the large spread in galactosylation and sialylation levels found between both strains and subclasses	1634:1744	Especially the large spread in galactosylation and sialylation levels found between both strains and subclasses may vastly influence IgG effector functions.
28620376	1	61	theme	N-linked	120:127	arg1	glycosylation					129:141	N-linked glycosylation	120:141	N-linked glycosylation of the fragment crystallizable (Fc)-region of immunoglobulin G (IgG)	120:210	N-linked glycosylation of the fragment crystallizable (Fc)-region of immunoglobulin G (IgG) is known to have a large influence on the activity of the antibody, an effect reported to be IgG subclass specific.
28620376	7	62	theme	bisecting	1477:1485	arg1	N-acetylglucosamine					1487:1505	a bisecting N-acetylglucosamine	1475:1505	a bisecting N-acetylglucosamine	1475:1505	In addition, we report glycosylation features not previously reported in an Fc-specific way on murine IgG, including monoantennary, hybrid, and high mannose structures, as well as diantennary structures without a core fucose, with a bisecting N-acetylglucosamine, or with α1,3-galactosylation.
28620376	6	63	theme	structural	1089:1098	arg1	analysis					1100:1107	The structural analysis	1085:1107	The structural analysis	1085:1107	The structural analysis revealed the presence of predominantly fucosylated, diantennary glycans, with varying amounts of galactosylation and α2,6-sialylation.
28620376	1	64	contain	have	224:227	arg2	influence					237:245	a large influence	229:245	a large influence	229:245	N-linked glycosylation of the fragment crystallizable (Fc)-region of immunoglobulin G (IgG) is known to have a large influence on the activity of the antibody, an effect reported to be IgG subclass specific.
28620376	1	64	contain	have	224:227	arg1	glycosylation					129:141	N-linked glycosylation	120:141	N-linked glycosylation of the fragment crystallizable (Fc)-region of immunoglobulin G (IgG)	120:210	N-linked glycosylation of the fragment crystallizable (Fc)-region of immunoglobulin G (IgG) is known to have a large influence on the activity of the antibody, an effect reported to be IgG subclass specific.
28620376	5	65	theme	glycosylation	803:815	arg1	differences					817:827	the systematic glycosylation differences	788:827	the systematic glycosylation differences between mouse strains, sexes, and IgG subclasses	788:876	To characterize the glycosylation profile of murine IgG-Fc and in addition evaluate the systematic glycosylation differences between mouse strains, sexes, and IgG subclasses, we used nanoliquid chromatography mass spectrometry (nanoLC-MS(/MS)) to look at the subclass-specific IgG Fc-glycopeptides of male and female mice from the strains BALB/c, C57BL/6, CD-1, and Swiss Webster.
28620376	7	66	from	way	1332:1334	arg1	IgG					1346:1348	murine IgG	1339:1348	murine IgG	1339:1348	In addition, we report glycosylation features not previously reported in an Fc-specific way on murine IgG, including monoantennary, hybrid, and high mannose structures, as well as diantennary structures without a core fucose, with a bisecting N-acetylglucosamine, or with α1,3-galactosylation.
28620376	1	67	theme	fragment	150:157	arg1	-region					178:184	the fragment crystallizable (Fc)-region	146:184	the fragment crystallizable (Fc)-region of immunoglobulin G (IgG)	146:210	N-linked glycosylation of the fragment crystallizable (Fc)-region of immunoglobulin G (IgG) is known to have a large influence on the activity of the antibody, an effect reported to be IgG subclass specific.
28620376	5	68	theme	male	1005:1008	arg1	mice					1021:1024	male and female mice	1005:1024	male and female mice	1005:1024	To characterize the glycosylation profile of murine IgG-Fc and in addition evaluate the systematic glycosylation differences between mouse strains, sexes, and IgG subclasses, we used nanoliquid chromatography mass spectrometry (nanoLC-MS(/MS)) to look at the subclass-specific IgG Fc-glycopeptides of male and female mice from the strains BALB/c, C57BL/6, CD-1, and Swiss Webster.
28620376	3	69	gly	Fc-glycosylation	453:468	arg1	IgG					473:475	IgG effector functions	473:494	IgG effector functions	473:494	The mouse is often used as experimental animal model to study the effects of Fc-glycosylation on IgG effector functions, and results are not uncommonly translated back to the human situation.
28620376	3	69	gly	Fc-glycosylation	453:468	arg1	effector					477:484	IgG effector functions	473:494	IgG effector functions	473:494	The mouse is often used as experimental animal model to study the effects of Fc-glycosylation on IgG effector functions, and results are not uncommonly translated back to the human situation.
28620376	0	70	theme	IgG	92:94	arg1	Subclasses					96:105	IgG Subclasses	92:105	IgG Subclasses	92:105	The N-Glycosylation of Mouse Immunoglobulin G (IgG)-Fragment Crystallizable Differs Between IgG Subclasses and Strains.
28620376	1	71	theme	IgG	305:307	arg1	subclass					309:316	IgG subclass	305:316	IgG subclass	305:316	N-linked glycosylation of the fragment crystallizable (Fc)-region of immunoglobulin G (IgG) is known to have a large influence on the activity of the antibody, an effect reported to be IgG subclass specific.
28620376	1	72	theme	immunoglobulin	189:202	arg1	IgG					207:209	IgG	207:209	IgG	207:209	N-linked glycosylation of the fragment crystallizable (Fc)-region of immunoglobulin G (IgG) is known to have a large influence on the activity of the antibody, an effect reported to be IgG subclass specific.
28620376	1	72	theme	immunoglobulin	189:202	arg1	G					204:204	immunoglobulin G	189:204	immunoglobulin G (IgG)	189:210	N-linked glycosylation of the fragment crystallizable (Fc)-region of immunoglobulin G (IgG) is known to have a large influence on the activity of the antibody, an effect reported to be IgG subclass specific.
28620376	5	73	theme	nanoliquid	887:896	arg1	spectrometry					918:929	nanoliquid chromatography mass spectrometry	887:929	nanoliquid chromatography mass spectrometry (nanoLC-MS(/MS))	887:946	To characterize the glycosylation profile of murine IgG-Fc and in addition evaluate the systematic glycosylation differences between mouse strains, sexes, and IgG subclasses, we used nanoliquid chromatography mass spectrometry (nanoLC-MS(/MS)) to look at the subclass-specific IgG Fc-glycopeptides of male and female mice from the strains BALB/c, C57BL/6, CD-1, and Swiss Webster.
28620376	5	74	theme	mice	1021:1024	arg1	Fc-glycopeptides					985:1000	the subclass-specific IgG Fc-glycopeptides	959:1000	the subclass-specific IgG Fc-glycopeptides of male and female mice from the strains BALB/c, C57BL/6, CD-1, and Swiss Webster	959:1082	To characterize the glycosylation profile of murine IgG-Fc and in addition evaluate the systematic glycosylation differences between mouse strains, sexes, and IgG subclasses, we used nanoliquid chromatography mass spectrometry (nanoLC-MS(/MS)) to look at the subclass-specific IgG Fc-glycopeptides of male and female mice from the strains BALB/c, C57BL/6, CD-1, and Swiss Webster.
28620376	5	75	dep	strains	1035:1041	arg1	strains					1035:1041	the strains BALB/c, C57BL/6, CD-1, and Swiss Webster	1031:1082	the strains BALB/c, C57BL/6, CD-1, and Swiss Webster	1031:1082	To characterize the glycosylation profile of murine IgG-Fc and in addition evaluate the systematic glycosylation differences between mouse strains, sexes, and IgG subclasses, we used nanoliquid chromatography mass spectrometry (nanoLC-MS(/MS)) to look at the subclass-specific IgG Fc-glycopeptides of male and female mice from the strains BALB/c, C57BL/6, CD-1, and Swiss Webster.
28620376	5	75	dep	strains	1035:1041	arg1	CD-1					1060:1063	CD-1	1060:1063	CD-1	1060:1063	To characterize the glycosylation profile of murine IgG-Fc and in addition evaluate the systematic glycosylation differences between mouse strains, sexes, and IgG subclasses, we used nanoliquid chromatography mass spectrometry (nanoLC-MS(/MS)) to look at the subclass-specific IgG Fc-glycopeptides of male and female mice from the strains BALB/c, C57BL/6, CD-1, and Swiss Webster.
28620376	5	75	dep	strains	1035:1041	arg1	Webster					1076:1082	Webster	1076:1082	Webster	1076:1082	To characterize the glycosylation profile of murine IgG-Fc and in addition evaluate the systematic glycosylation differences between mouse strains, sexes, and IgG subclasses, we used nanoliquid chromatography mass spectrometry (nanoLC-MS(/MS)) to look at the subclass-specific IgG Fc-glycopeptides of male and female mice from the strains BALB/c, C57BL/6, CD-1, and Swiss Webster.
28620376	5	75	dep	strains	1035:1041	arg1	C57BL/6					1051:1057	C57BL/6	1051:1057	C57BL/6	1051:1057	To characterize the glycosylation profile of murine IgG-Fc and in addition evaluate the systematic glycosylation differences between mouse strains, sexes, and IgG subclasses, we used nanoliquid chromatography mass spectrometry (nanoLC-MS(/MS)) to look at the subclass-specific IgG Fc-glycopeptides of male and female mice from the strains BALB/c, C57BL/6, CD-1, and Swiss Webster.
28620376	5	75	dep	strains	1035:1041	arg1	BALB/c					1043:1048	BALB/c	1043:1048	BALB/c	1043:1048	To characterize the glycosylation profile of murine IgG-Fc and in addition evaluate the systematic glycosylation differences between mouse strains, sexes, and IgG subclasses, we used nanoliquid chromatography mass spectrometry (nanoLC-MS(/MS)) to look at the subclass-specific IgG Fc-glycopeptides of male and female mice from the strains BALB/c, C57BL/6, CD-1, and Swiss Webster.
28620376	0	76	theme	Mouse	23:27	arg1	G					44:44	Mouse Immunoglobulin G	23:44	Mouse Immunoglobulin G (IgG)-Fragment Crystallizable	23:74	The N-Glycosylation of Mouse Immunoglobulin G (IgG)-Fragment Crystallizable Differs Between IgG Subclasses and Strains.
28620376	0	76	theme	Mouse	23:27	arg1	IgG					47:49	IgG	47:49	IgG	47:49	The N-Glycosylation of Mouse Immunoglobulin G (IgG)-Fragment Crystallizable Differs Between IgG Subclasses and Strains.
28620376	3	77	used	used	395:398	arg2	mouse					380:384	The mouse	376:384	The mouse	376:384	The mouse is often used as experimental animal model to study the effects of Fc-glycosylation on IgG effector functions, and results are not uncommonly translated back to the human situation.
28620376	3	77	used	used	395:398	arg2	model					423:427	experimental animal model	403:427	experimental animal model	403:427	The mouse is often used as experimental animal model to study the effects of Fc-glycosylation on IgG effector functions, and results are not uncommonly translated back to the human situation.
28620376	5	78	from	strains	1035:1041	arg1	Fc-glycopeptides					985:1000	the subclass-specific IgG Fc-glycopeptides	959:1000	the subclass-specific IgG Fc-glycopeptides of male and female mice from the strains BALB/c, C57BL/6, CD-1, and Swiss Webster	959:1082	To characterize the glycosylation profile of murine IgG-Fc and in addition evaluate the systematic glycosylation differences between mouse strains, sexes, and IgG subclasses, we used nanoliquid chromatography mass spectrometry (nanoLC-MS(/MS)) to look at the subclass-specific IgG Fc-glycopeptides of male and female mice from the strains BALB/c, C57BL/6, CD-1, and Swiss Webster.
28620376	9	79	theme	IgG	1767:1769	arg1	functions					1780:1788	IgG effector functions	1767:1788	IgG effector functions	1767:1788	Especially the large spread in galactosylation and sialylation levels found between both strains and subclasses may vastly influence IgG effector functions.
28620376	7	80	with	features	1281:1288	arg1	α1,3-galactosylation					1516:1535	α1,3-galactosylation	1516:1535	α1,3-galactosylation	1516:1535	In addition, we report glycosylation features not previously reported in an Fc-specific way on murine IgG, including monoantennary, hybrid, and high mannose structures, as well as diantennary structures without a core fucose, with a bisecting N-acetylglucosamine, or with α1,3-galactosylation.
28620376	7	80	with	features	1281:1288	arg1	N-acetylglucosamine					1487:1505	a bisecting N-acetylglucosamine	1475:1505	a bisecting N-acetylglucosamine	1475:1505	In addition, we report glycosylation features not previously reported in an Fc-specific way on murine IgG, including monoantennary, hybrid, and high mannose structures, as well as diantennary structures without a core fucose, with a bisecting N-acetylglucosamine, or with α1,3-galactosylation.
28620376	7	81	theme	diantennary	1424:1434	arg1	structures					1436:1445	diantennary structures	1424:1445	diantennary structures without a core fucose, with a bisecting N-acetylglucosamine, or with α1,3-galactosylation	1424:1535	In addition, we report glycosylation features not previously reported in an Fc-specific way on murine IgG, including monoantennary, hybrid, and high mannose structures, as well as diantennary structures without a core fucose, with a bisecting N-acetylglucosamine, or with α1,3-galactosylation.
28620376	5	82	theme	mass	913:916	arg1	spectrometry					918:929	nanoliquid chromatography mass spectrometry	887:929	nanoliquid chromatography mass spectrometry (nanoLC-MS(/MS))	887:946	To characterize the glycosylation profile of murine IgG-Fc and in addition evaluate the systematic glycosylation differences between mouse strains, sexes, and IgG subclasses, we used nanoliquid chromatography mass spectrometry (nanoLC-MS(/MS)) to look at the subclass-specific IgG Fc-glycopeptides of male and female mice from the strains BALB/c, C57BL/6, CD-1, and Swiss Webster.
28620376	10	83	theme	glycosylation	1832:1844	arg1	differences					1846:1856	Mouse strain-based and subclass-specific glycosylation differences	1791:1856	differences	1846:1856	Mouse strain-based and subclass-specific glycosylation differences should be taken into account when designing and interpreting immunological and glycobiological mouse studies involving IgG effector functions.
28620376	3	84	theme	animal	416:421	arg1	model					423:427	experimental animal model	403:427	experimental animal model	403:427	The mouse is often used as experimental animal model to study the effects of Fc-glycosylation on IgG effector functions, and results are not uncommonly translated back to the human situation.
28620376	3	84	theme	animal	416:421	arg1	mouse					380:384	The mouse	376:384	The mouse	376:384	The mouse is often used as experimental animal model to study the effects of Fc-glycosylation on IgG effector functions, and results are not uncommonly translated back to the human situation.
28620376	6	85	dep	fucosylated	1148:1158	arg1	diantennary					1161:1171	diantennary	1161:1171	diantennary	1161:1171	The structural analysis revealed the presence of predominantly fucosylated, diantennary glycans, with varying amounts of galactosylation and α2,6-sialylation.
28620376	5	86	gly	Fc-glycopeptides	985:1000	arg1	strains					1035:1041	the strains BALB/c, C57BL/6, CD-1, and Swiss Webster	1031:1082	the strains BALB/c, C57BL/6, CD-1, and Swiss Webster	1031:1082	To characterize the glycosylation profile of murine IgG-Fc and in addition evaluate the systematic glycosylation differences between mouse strains, sexes, and IgG subclasses, we used nanoliquid chromatography mass spectrometry (nanoLC-MS(/MS)) to look at the subclass-specific IgG Fc-glycopeptides of male and female mice from the strains BALB/c, C57BL/6, CD-1, and Swiss Webster.
28620376	5	86	gly	Fc-glycopeptides	985:1000	arg1	BALB/c					1043:1048	BALB/c	1043:1048	BALB/c	1043:1048	To characterize the glycosylation profile of murine IgG-Fc and in addition evaluate the systematic glycosylation differences between mouse strains, sexes, and IgG subclasses, we used nanoliquid chromatography mass spectrometry (nanoLC-MS(/MS)) to look at the subclass-specific IgG Fc-glycopeptides of male and female mice from the strains BALB/c, C57BL/6, CD-1, and Swiss Webster.
28620376	5	86	gly	Fc-glycopeptides	985:1000	arg2	Fc-glycopeptides					985:1000	the subclass-specific IgG Fc-glycopeptides	959:1000	the subclass-specific IgG Fc-glycopeptides of male and female mice from the strains BALB/c, C57BL/6, CD-1, and Swiss Webster	959:1082	To characterize the glycosylation profile of murine IgG-Fc and in addition evaluate the systematic glycosylation differences between mouse strains, sexes, and IgG subclasses, we used nanoliquid chromatography mass spectrometry (nanoLC-MS(/MS)) to look at the subclass-specific IgG Fc-glycopeptides of male and female mice from the strains BALB/c, C57BL/6, CD-1, and Swiss Webster.
28620376	5	86	gly	Fc-glycopeptides	985:1000	arg1	Webster					1076:1082	Webster	1076:1082	Webster	1076:1082	To characterize the glycosylation profile of murine IgG-Fc and in addition evaluate the systematic glycosylation differences between mouse strains, sexes, and IgG subclasses, we used nanoliquid chromatography mass spectrometry (nanoLC-MS(/MS)) to look at the subclass-specific IgG Fc-glycopeptides of male and female mice from the strains BALB/c, C57BL/6, CD-1, and Swiss Webster.
28620376	5	86	gly	Fc-glycopeptides	985:1000	arg1	mice					1021:1024	male and female mice	1005:1024	male and female mice	1005:1024	To characterize the glycosylation profile of murine IgG-Fc and in addition evaluate the systematic glycosylation differences between mouse strains, sexes, and IgG subclasses, we used nanoliquid chromatography mass spectrometry (nanoLC-MS(/MS)) to look at the subclass-specific IgG Fc-glycopeptides of male and female mice from the strains BALB/c, C57BL/6, CD-1, and Swiss Webster.
28620376	5	86	gly	Fc-glycopeptides	985:1000	arg1	C57BL/6					1051:1057	C57BL/6	1051:1057	C57BL/6	1051:1057	To characterize the glycosylation profile of murine IgG-Fc and in addition evaluate the systematic glycosylation differences between mouse strains, sexes, and IgG subclasses, we used nanoliquid chromatography mass spectrometry (nanoLC-MS(/MS)) to look at the subclass-specific IgG Fc-glycopeptides of male and female mice from the strains BALB/c, C57BL/6, CD-1, and Swiss Webster.
28620376	5	86	gly	Fc-glycopeptides	985:1000	arg1	CD-1					1060:1063	CD-1	1060:1063	CD-1	1060:1063	To characterize the glycosylation profile of murine IgG-Fc and in addition evaluate the systematic glycosylation differences between mouse strains, sexes, and IgG subclasses, we used nanoliquid chromatography mass spectrometry (nanoLC-MS(/MS)) to look at the subclass-specific IgG Fc-glycopeptides of male and female mice from the strains BALB/c, C57BL/6, CD-1, and Swiss Webster.
28620376	8	87	theme	IgG	1599:1601	arg1	subclasses					1603:1612	the IgG subclasses	1595:1612	the IgG subclasses	1595:1612	Pronounced differences were detected between strains and the IgG subclasses within each strain.
28620376	10	88	theme	IgG	1977:1979	arg1	functions					1990:1998	IgG effector functions	1977:1998	IgG effector functions	1977:1998	Mouse strain-based and subclass-specific glycosylation differences should be taken into account when designing and interpreting immunological and glycobiological mouse studies involving IgG effector functions.
27418570	10	0	theme	biomimetic	1712:1721	arg1	hydroxyapatite					1723:1736	biomimetic hydroxyapatite	1712:1736	biomimetic hydroxyapatite	1712:1736	The best compromise should therefore go, in our opinion, to zones with intermediate-to-high chitosan concentration which can assure a large spectrum of antimicrobial protection concomitantly with a significant enhancement of osseointegration, favored by the presence of biomimetic hydroxyapatite.
27418570	6	1	theme	round	983:987	arg1	grains					996:1001	nanometric round shaped grains	972:1001	nanometric round shaped grains	972:1001	The obtained thin films were XRD amorphous and exhibited a morphology characteristic to the laser deposited structures composed of nanometric round shaped grains.
27418570	5	2	theme	antimicrobial	670:682	arg1	efficiency					684:693	The antimicrobial efficiency	666:693	The antimicrobial efficiency of the chitosan/biomimetic apatite layers against Staphylococcus aureus and Escherichia coli strains	666:794	The antimicrobial efficiency of the chitosan/biomimetic apatite layers against Staphylococcus aureus and Escherichia coli strains was interrogated by viable cell count assay.
27418570	1	3	theme	mild	174:177	arg1	conditions					179:188	mild conditions	174:188	mild conditions of temperature and pressure	174:216	Chitosan/biomimetic apatite thin films were grown in mild conditions of temperature and pressure by Combinatorial Matrix-Assisted Pulsed Laser Evaporation on Ti, Si or glass substrates.
27418570	8	4	theme	films	1175:1179	arg1	composition					1129:1139	the composition	1125:1139	the composition of the BmAp-CHT C-MAPLE composite films	1125:1179	FTIR, EDS and XPS analyses indicated that the composition of the BmAp-CHT C-MAPLE composite films gradually modified from pure apatite to chitosan.
27418570	9	5	theme	chitosan-rich	1325:1337	arg1	areas					1339:1343	chitosan-rich areas	1325:1343	chitosan-rich areas of the films	1325:1356	The bioevaluation tests indicated that S. aureus biofilm is more susceptible to the action of chitosan-rich areas of the films, whilst the E. coli biofilm proved more sensible to areas containing less chitosan.
27418570	10	6	theme	hydroxyapatite	1723:1736	arg1	presence					1700:1707	the presence	1696:1707	the presence of biomimetic hydroxyapatite	1696:1736	The best compromise should therefore go, in our opinion, to zones with intermediate-to-high chitosan concentration which can assure a large spectrum of antimicrobial protection concomitantly with a significant enhancement of osseointegration, favored by the presence of biomimetic hydroxyapatite.
27418570	5	7	theme	apatite	722:728	arg1	layers					730:735	the chitosan/biomimetic apatite layers	698:735	the chitosan/biomimetic apatite layers	698:735	The antimicrobial efficiency of the chitosan/biomimetic apatite layers against Staphylococcus aureus and Escherichia coli strains was interrogated by viable cell count assay.
27418570	6	8	theme	characteristic	911:924	arg1	morphology					900:909	a morphology	898:909	a morphology characteristic to the laser deposited structures composed of nanometric round shaped grains	898:1001	The obtained thin films were XRD amorphous and exhibited a morphology characteristic to the laser deposited structures composed of nanometric round shaped grains.
27418570	8	9	theme	FTIR	1083:1086	arg1	analyses					1101:1108	FTIR, EDS and XPS analyses	1083:1108	analyses	1101:1108	FTIR, EDS and XPS analyses indicated that the composition of the BmAp-CHT C-MAPLE composite films gradually modified from pure apatite to chitosan.
27418570	9	10	contain	containing	1416:1425	arg1	areas					1410:1414	areas	1410:1414	areas containing less chitosan	1410:1439	The bioevaluation tests indicated that S. aureus biofilm is more susceptible to the action of chitosan-rich areas of the films, whilst the E. coli biofilm proved more sensible to areas containing less chitosan.
27418570	9	10	contain	containing	1416:1425	arg2	chitosan					1432:1439	less chitosan	1427:1439	less chitosan	1427:1439	The bioevaluation tests indicated that S. aureus biofilm is more susceptible to the action of chitosan-rich areas of the films, whilst the E. coli biofilm proved more sensible to areas containing less chitosan.
27418570	10	11	theme	protection	1608:1617	arg1	spectrum					1582:1589	a large spectrum	1574:1589	a large spectrum of antimicrobial protection	1574:1617	The best compromise should therefore go, in our opinion, to zones with intermediate-to-high chitosan concentration which can assure a large spectrum of antimicrobial protection concomitantly with a significant enhancement of osseointegration, favored by the presence of biomimetic hydroxyapatite.
27418570	9	12	theme	areas	1339:1343	arg1	action					1315:1320	the action	1311:1320	the action of chitosan-rich areas of the films	1311:1356	The bioevaluation tests indicated that S. aureus biofilm is more susceptible to the action of chitosan-rich areas of the films, whilst the E. coli biofilm proved more sensible to areas containing less chitosan.
27418570	0	13	theme	apatite	104:110	arg1	powders					112:118	apatite powders	104:118	apatite powders	104:118	Combinatorial MAPLE deposition of antimicrobial orthopedic maps fabricated from chitosan and biomimetic apatite powders.
27418570	2	14	theme	Compositional	307:319	arg1	gradients					321:329	Compositional gradients	307:329	Compositional gradients	307:329	Compositional gradients were obtained by simultaneous laser vaporization of the two distinct material targets.
27418570	1	15	theme	glass	289:293	arg1	substrates					295:304	glass substrates	289:304	glass substrates	289:304	Chitosan/biomimetic apatite thin films were grown in mild conditions of temperature and pressure by Combinatorial Matrix-Assisted Pulsed Laser Evaporation on Ti, Si or glass substrates.
27418570	8	16	theme	EDS	1089:1091	arg1	analyses					1101:1108	FTIR, EDS and XPS analyses	1083:1108	analyses	1101:1108	FTIR, EDS and XPS analyses indicated that the composition of the BmAp-CHT C-MAPLE composite films gradually modified from pure apatite to chitosan.
27418570	5	17	theme	viable	816:821	arg1	count					828:832	viable cell count	816:832	viable cell count assay	816:838	The antimicrobial efficiency of the chitosan/biomimetic apatite layers against Staphylococcus aureus and Escherichia coli strains was interrogated by viable cell count assay.
27418570	10	18	theme	intermediate-to-high	1513:1532	arg1	concentration					1543:1555	intermediate-to-high chitosan concentration	1513:1555	intermediate-to-high chitosan concentration which can assure a large spectrum of antimicrobial protection concomitantly with a significant enhancement of osseointegration, favored by the presence of biomimetic hydroxyapatite	1513:1736	The best compromise should therefore go, in our opinion, to zones with intermediate-to-high chitosan concentration which can assure a large spectrum of antimicrobial protection concomitantly with a significant enhancement of osseointegration, favored by the presence of biomimetic hydroxyapatite.
27418570	3	19	theme	laser	455:459	arg1	source					461:466	A KrF* excimer (λ=248nm, τFWHM=25ns) laser source	418:466	source	461:466	A KrF* excimer (λ=248nm, τFWHM=25ns) laser source was used in all experiments.
27418570	8	20	theme	pure	1205:1208	arg1	apatite					1210:1216	pure apatite	1205:1216	pure apatite to chitosan	1205:1228	FTIR, EDS and XPS analyses indicated that the composition of the BmAp-CHT C-MAPLE composite films gradually modified from pure apatite to chitosan.
27418570	9	21	theme	films	1352:1356	arg1	areas					1339:1343	chitosan-rich areas	1325:1343	chitosan-rich areas of the films	1325:1356	The bioevaluation tests indicated that S. aureus biofilm is more susceptible to the action of chitosan-rich areas of the films, whilst the E. coli biofilm proved more sensible to areas containing less chitosan.
27418570	3	22	theme	λ=248nm	434:440	arg1	source					461:466	A KrF* excimer (λ=248nm, τFWHM=25ns) laser source	418:466	source	461:466	A KrF* excimer (λ=248nm, τFWHM=25ns) laser source was used in all experiments.
27418570	9	23	theme	bioevaluation	1235:1247	arg1	tests					1249:1253	The bioevaluation tests	1231:1253	The bioevaluation tests	1231:1253	The bioevaluation tests indicated that S. aureus biofilm is more susceptible to the action of chitosan-rich areas of the films, whilst the E. coli biofilm proved more sensible to areas containing less chitosan.
27418570	3	24	theme	*	423:423	arg1	source					461:466	A KrF* excimer (λ=248nm, τFWHM=25ns) laser source	418:466	source	461:466	A KrF* excimer (λ=248nm, τFWHM=25ns) laser source was used in all experiments.
27418570	10	25	theme	large	1576:1580	arg1	spectrum					1582:1589	a large spectrum	1574:1589	a large spectrum of antimicrobial protection	1574:1617	The best compromise should therefore go, in our opinion, to zones with intermediate-to-high chitosan concentration which can assure a large spectrum of antimicrobial protection concomitantly with a significant enhancement of osseointegration, favored by the presence of biomimetic hydroxyapatite.
27418570	1	26	theme	temperature	193:203	arg1	conditions					179:188	mild conditions	174:188	mild conditions of temperature and pressure	174:216	Chitosan/biomimetic apatite thin films were grown in mild conditions of temperature and pressure by Combinatorial Matrix-Assisted Pulsed Laser Evaporation on Ti, Si or glass substrates.
27418570	0	27	theme	MAPLE	14:18	arg1	deposition					20:29	Combinatorial MAPLE deposition	0:29	Combinatorial MAPLE deposition of antimicrobial orthopedic maps	0:62	Combinatorial MAPLE deposition of antimicrobial orthopedic maps fabricated from chitosan and biomimetic apatite powders.
27418570	8	28	theme	XPS	1097:1099	arg1	analyses					1101:1108	FTIR, EDS and XPS analyses	1083:1108	analyses	1101:1108	FTIR, EDS and XPS analyses indicated that the composition of the BmAp-CHT C-MAPLE composite films gradually modified from pure apatite to chitosan.
27418570	8	29	theme	composite	1165:1173	arg1	films					1175:1179	the BmAp-CHT C-MAPLE composite films	1144:1179	the BmAp-CHT C-MAPLE composite films	1144:1179	FTIR, EDS and XPS analyses indicated that the composition of the BmAp-CHT C-MAPLE composite films gradually modified from pure apatite to chitosan.
27418570	2	30	theme	simultaneous	348:359	arg1	vaporization					367:378	simultaneous laser vaporization	348:378	simultaneous laser vaporization of the two distinct material targets	348:415	Compositional gradients were obtained by simultaneous laser vaporization of the two distinct material targets.
27418570	0	31	theme	Combinatorial	0:12	arg1	deposition					20:29	Combinatorial MAPLE deposition	0:29	Combinatorial MAPLE deposition of antimicrobial orthopedic maps	0:62	Combinatorial MAPLE deposition of antimicrobial orthopedic maps fabricated from chitosan and biomimetic apatite powders.
27418570	4	32	dep	nature	501:506	arg1	The					497:499	The	497:499	The	497:499	The nature and surface composition of deposited materials and the spatial distribution of constituents were studied by SEM, EDS, AFM, GIXRD, FTIR, micro-Raman, and XPS.
27418570	5	33	theme	cell	823:826	arg1	count					828:832	viable cell count	816:832	viable cell count assay	816:838	The antimicrobial efficiency of the chitosan/biomimetic apatite layers against Staphylococcus aureus and Escherichia coli strains was interrogated by viable cell count assay.
27418570	1	34	theme	pressure	209:216	arg1	conditions					179:188	mild conditions	174:188	mild conditions of temperature and pressure	174:216	Chitosan/biomimetic apatite thin films were grown in mild conditions of temperature and pressure by Combinatorial Matrix-Assisted Pulsed Laser Evaporation on Ti, Si or glass substrates.
27418570	5	35	theme	count	828:832	arg1	assay					834:838	viable cell count assay	816:838	viable cell count assay	816:838	The antimicrobial efficiency of the chitosan/biomimetic apatite layers against Staphylococcus aureus and Escherichia coli strains was interrogated by viable cell count assay.
27418570	4	36	theme	spatial	563:569	arg1	distribution					571:582	the spatial distribution	559:582	the spatial distribution of constituents	559:598	The nature and surface composition of deposited materials and the spatial distribution of constituents were studied by SEM, EDS, AFM, GIXRD, FTIR, micro-Raman, and XPS.
27418570	0	37	dep	chitosan	80:87	arg1	powders					112:118	apatite powders	104:118	apatite powders	104:118	Combinatorial MAPLE deposition of antimicrobial orthopedic maps fabricated from chitosan and biomimetic apatite powders.
27418570	9	38	theme	aureus	1273:1278	arg1	biofilm					1280:1286	S. aureus biofilm	1270:1286	S. aureus biofilm	1270:1286	The bioevaluation tests indicated that S. aureus biofilm is more susceptible to the action of chitosan-rich areas of the films, whilst the E. coli biofilm proved more sensible to areas containing less chitosan.
27418570	4	39	theme	constituents	587:598	arg1	distribution					571:582	the spatial distribution	559:582	the spatial distribution of constituents	559:598	The nature and surface composition of deposited materials and the spatial distribution of constituents were studied by SEM, EDS, AFM, GIXRD, FTIR, micro-Raman, and XPS.
27418570	4	39	theme	constituents	587:598	arg1	nature					501:506	nature	501:506	nature	501:506	The nature and surface composition of deposited materials and the spatial distribution of constituents were studied by SEM, EDS, AFM, GIXRD, FTIR, micro-Raman, and XPS.
27418570	4	39	theme	constituents	587:598	arg1	composition					520:530	surface composition	512:530	surface composition	512:530	The nature and surface composition of deposited materials and the spatial distribution of constituents were studied by SEM, EDS, AFM, GIXRD, FTIR, micro-Raman, and XPS.
27418570	9	40	theme	S.	1270:1271	arg1	biofilm					1280:1286	S. aureus biofilm	1270:1286	S. aureus biofilm	1270:1286	The bioevaluation tests indicated that S. aureus biofilm is more susceptible to the action of chitosan-rich areas of the films, whilst the E. coli biofilm proved more sensible to areas containing less chitosan.
27418570	1	41	theme	Chitosan/biomimetic	121:139	arg1	films					154:158	Chitosan/biomimetic apatite thin films	121:158	Chitosan/biomimetic apatite thin films	121:158	Chitosan/biomimetic apatite thin films were grown in mild conditions of temperature and pressure by Combinatorial Matrix-Assisted Pulsed Laser Evaporation on Ti, Si or glass substrates.
27418570	1	42	theme	Combinatorial	221:233	arg1	Evaporation					264:274	Combinatorial Matrix-Assisted Pulsed Laser Evaporation	221:274	Combinatorial Matrix-Assisted Pulsed Laser Evaporation on Ti, Si or glass substrates	221:304	Chitosan/biomimetic apatite thin films were grown in mild conditions of temperature and pressure by Combinatorial Matrix-Assisted Pulsed Laser Evaporation on Ti, Si or glass substrates.
27418570	0	43	theme	orthopedic	48:57	arg1	maps					59:62	antimicrobial orthopedic maps	34:62	antimicrobial orthopedic maps	34:62	Combinatorial MAPLE deposition of antimicrobial orthopedic maps fabricated from chitosan and biomimetic apatite powders.
27418570	6	44	theme	nanometric	972:981	arg1	grains					996:1001	nanometric round shaped grains	972:1001	nanometric round shaped grains	972:1001	The obtained thin films were XRD amorphous and exhibited a morphology characteristic to the laser deposited structures composed of nanometric round shaped grains.
27418570	5	45	dep	aureus	760:765	arg1	strains					788:794	strains	788:794	strains	788:794	The antimicrobial efficiency of the chitosan/biomimetic apatite layers against Staphylococcus aureus and Escherichia coli strains was interrogated by viable cell count assay.
27418570	6	46	theme	XRD	870:872	arg1	films					859:863	The obtained thin films	841:863	The obtained thin films	841:863	The obtained thin films were XRD amorphous and exhibited a morphology characteristic to the laser deposited structures composed of nanometric round shaped grains.
27418570	6	46	theme	XRD	870:872	arg1	amorphous					874:882	XRD amorphous	870:882	XRD amorphous	870:882	The obtained thin films were XRD amorphous and exhibited a morphology characteristic to the laser deposited structures composed of nanometric round shaped grains.
27418570	10	47	with	zones	1502:1506	arg1	concentration					1543:1555	intermediate-to-high chitosan concentration	1513:1555	intermediate-to-high chitosan concentration which can assure a large spectrum of antimicrobial protection concomitantly with a significant enhancement of osseointegration, favored by the presence of biomimetic hydroxyapatite	1513:1736	The best compromise should therefore go, in our opinion, to zones with intermediate-to-high chitosan concentration which can assure a large spectrum of antimicrobial protection concomitantly with a significant enhancement of osseointegration, favored by the presence of biomimetic hydroxyapatite.
27418570	1	48	theme	Matrix-Assisted	235:249	arg1	Evaporation					264:274	Combinatorial Matrix-Assisted Pulsed Laser Evaporation	221:274	Combinatorial Matrix-Assisted Pulsed Laser Evaporation on Ti, Si or glass substrates	221:304	Chitosan/biomimetic apatite thin films were grown in mild conditions of temperature and pressure by Combinatorial Matrix-Assisted Pulsed Laser Evaporation on Ti, Si or glass substrates.
27418570	0	49	theme	antimicrobial	34:46	arg1	maps					59:62	antimicrobial orthopedic maps	34:62	antimicrobial orthopedic maps	34:62	Combinatorial MAPLE deposition of antimicrobial orthopedic maps fabricated from chitosan and biomimetic apatite powders.
27418570	2	50	theme	distinct	391:398	arg1	targets					409:415	the two distinct material targets	383:415	the two distinct material targets	383:415	Compositional gradients were obtained by simultaneous laser vaporization of the two distinct material targets.
27418570	5	51	theme	chitosan/biomimetic	702:720	arg1	layers					730:735	the chitosan/biomimetic apatite layers	698:735	the chitosan/biomimetic apatite layers	698:735	The antimicrobial efficiency of the chitosan/biomimetic apatite layers against Staphylococcus aureus and Escherichia coli strains was interrogated by viable cell count assay.
27418570	5	52	theme	layers	730:735	arg1	efficiency					684:693	The antimicrobial efficiency	666:693	The antimicrobial efficiency of the chitosan/biomimetic apatite layers against Staphylococcus aureus and Escherichia coli strains	666:794	The antimicrobial efficiency of the chitosan/biomimetic apatite layers against Staphylococcus aureus and Escherichia coli strains was interrogated by viable cell count assay.
27418570	1	53	theme	Pulsed	251:256	arg1	Evaporation					264:274	Combinatorial Matrix-Assisted Pulsed Laser Evaporation	221:274	Combinatorial Matrix-Assisted Pulsed Laser Evaporation on Ti, Si or glass substrates	221:304	Chitosan/biomimetic apatite thin films were grown in mild conditions of temperature and pressure by Combinatorial Matrix-Assisted Pulsed Laser Evaporation on Ti, Si or glass substrates.
27418570	3	54	theme	excimer	425:431	arg1	source					461:466	A KrF* excimer (λ=248nm, τFWHM=25ns) laser source	418:466	source	461:466	A KrF* excimer (λ=248nm, τFWHM=25ns) laser source was used in all experiments.
27418570	4	55	theme	materials	545:553	arg1	distribution					571:582	the spatial distribution	559:582	the spatial distribution of constituents	559:598	The nature and surface composition of deposited materials and the spatial distribution of constituents were studied by SEM, EDS, AFM, GIXRD, FTIR, micro-Raman, and XPS.
27418570	4	55	theme	materials	545:553	arg1	nature					501:506	nature	501:506	nature	501:506	The nature and surface composition of deposited materials and the spatial distribution of constituents were studied by SEM, EDS, AFM, GIXRD, FTIR, micro-Raman, and XPS.
27418570	4	55	theme	materials	545:553	arg1	composition					520:530	surface composition	512:530	surface composition	512:530	The nature and surface composition of deposited materials and the spatial distribution of constituents were studied by SEM, EDS, AFM, GIXRD, FTIR, micro-Raman, and XPS.
27418570	6	56	dep	deposited	939:947	arg1	laser					933:937	laser	933:937	laser	933:937	The obtained thin films were XRD amorphous and exhibited a morphology characteristic to the laser deposited structures composed of nanometric round shaped grains.
27418570	6	57	theme	shaped	989:994	arg1	grains					996:1001	nanometric round shaped grains	972:1001	nanometric round shaped grains	972:1001	The obtained thin films were XRD amorphous and exhibited a morphology characteristic to the laser deposited structures composed of nanometric round shaped grains.
27418570	8	58	theme	BmAp-CHT	1148:1155	arg1	films					1175:1179	the BmAp-CHT C-MAPLE composite films	1144:1179	the BmAp-CHT C-MAPLE composite films	1144:1179	FTIR, EDS and XPS analyses indicated that the composition of the BmAp-CHT C-MAPLE composite films gradually modified from pure apatite to chitosan.
27418570	1	59	theme	apatite	141:147	arg1	films					154:158	Chitosan/biomimetic apatite thin films	121:158	Chitosan/biomimetic apatite thin films	121:158	Chitosan/biomimetic apatite thin films were grown in mild conditions of temperature and pressure by Combinatorial Matrix-Assisted Pulsed Laser Evaporation on Ti, Si or glass substrates.
27418570	0	60	theme	maps	59:62	arg1	deposition					20:29	Combinatorial MAPLE deposition	0:29	Combinatorial MAPLE deposition of antimicrobial orthopedic maps	0:62	Combinatorial MAPLE deposition of antimicrobial orthopedic maps fabricated from chitosan and biomimetic apatite powders.
27418570	9	61	dep	E.	1370:1371	arg1	coli					1373:1376	coli	1373:1376	coli	1373:1376	The bioevaluation tests indicated that S. aureus biofilm is more susceptible to the action of chitosan-rich areas of the films, whilst the E. coli biofilm proved more sensible to areas containing less chitosan.
27418570	6	62	theme	thin	854:857	arg1	films					859:863	The obtained thin films	841:863	The obtained thin films	841:863	The obtained thin films were XRD amorphous and exhibited a morphology characteristic to the laser deposited structures composed of nanometric round shaped grains.
27418570	6	62	theme	thin	854:857	arg1	amorphous					874:882	XRD amorphous	870:882	XRD amorphous	870:882	The obtained thin films were XRD amorphous and exhibited a morphology characteristic to the laser deposited structures composed of nanometric round shaped grains.
27418570	3	63	dep	source	461:466	arg1	τFWHM=25ns					443:452	A KrF* excimer (λ=248nm, τFWHM=25ns) laser source	418:466	τFWHM=25ns	443:452	A KrF* excimer (λ=248nm, τFWHM=25ns) laser source was used in all experiments.
27418570	1	64	theme	thin	149:152	arg1	films					154:158	Chitosan/biomimetic apatite thin films	121:158	Chitosan/biomimetic apatite thin films	121:158	Chitosan/biomimetic apatite thin films were grown in mild conditions of temperature and pressure by Combinatorial Matrix-Assisted Pulsed Laser Evaporation on Ti, Si or glass substrates.
27418570	6	65	theme	deposited	939:947	arg1	structures					949:958	the laser deposited structures	929:958	the laser deposited structures composed of nanometric round shaped grains	929:1001	The obtained thin films were XRD amorphous and exhibited a morphology characteristic to the laser deposited structures composed of nanometric round shaped grains.
27418570	7	66	theme	chitosan	1059:1066	arg1	concentration					1068:1080	chitosan concentration	1059:1080	chitosan concentration	1059:1080	The surface roughness has progressively increased with chitosan concentration.
27418570	6	67	theme	obtained	845:852	arg1	films					859:863	The obtained thin films	841:863	The obtained thin films	841:863	The obtained thin films were XRD amorphous and exhibited a morphology characteristic to the laser deposited structures composed of nanometric round shaped grains.
27418570	6	67	theme	obtained	845:852	arg1	amorphous					874:882	XRD amorphous	870:882	XRD amorphous	870:882	The obtained thin films were XRD amorphous and exhibited a morphology characteristic to the laser deposited structures composed of nanometric round shaped grains.
27418570	10	68	theme	antimicrobial	1594:1606	arg1	protection					1608:1617	antimicrobial protection	1594:1617	antimicrobial protection	1594:1617	The best compromise should therefore go, in our opinion, to zones with intermediate-to-high chitosan concentration which can assure a large spectrum of antimicrobial protection concomitantly with a significant enhancement of osseointegration, favored by the presence of biomimetic hydroxyapatite.
27418570	9	69	theme	E.	1370:1371	arg1	biofilm					1378:1384	the E. coli biofilm	1366:1384	the E. coli biofilm	1366:1384	The bioevaluation tests indicated that S. aureus biofilm is more susceptible to the action of chitosan-rich areas of the films, whilst the E. coli biofilm proved more sensible to areas containing less chitosan.
27418570	1	70	theme	Laser	258:262	arg1	Evaporation					264:274	Combinatorial Matrix-Assisted Pulsed Laser Evaporation	221:274	Combinatorial Matrix-Assisted Pulsed Laser Evaporation on Ti, Si or glass substrates	221:304	Chitosan/biomimetic apatite thin films were grown in mild conditions of temperature and pressure by Combinatorial Matrix-Assisted Pulsed Laser Evaporation on Ti, Si or glass substrates.
27418570	1	71	from	Evaporation	264:274	arg1	Si					283:284	Si	283:284	Si	283:284	Chitosan/biomimetic apatite thin films were grown in mild conditions of temperature and pressure by Combinatorial Matrix-Assisted Pulsed Laser Evaporation on Ti, Si or glass substrates.
27418570	1	71	from	Evaporation	264:274	arg1	substrates					295:304	glass substrates	289:304	glass substrates	289:304	Chitosan/biomimetic apatite thin films were grown in mild conditions of temperature and pressure by Combinatorial Matrix-Assisted Pulsed Laser Evaporation on Ti, Si or glass substrates.
27418570	1	71	from	Evaporation	264:274	arg1	Ti					279:280	Ti	279:280	Ti	279:280	Chitosan/biomimetic apatite thin films were grown in mild conditions of temperature and pressure by Combinatorial Matrix-Assisted Pulsed Laser Evaporation on Ti, Si or glass substrates.
27418570	7	72	theme	surface	1008:1014	arg1	roughness					1016:1024	The surface roughness	1004:1024	The surface roughness	1004:1024	The surface roughness has progressively increased with chitosan concentration.
27418570	2	73	theme	laser	361:365	arg1	vaporization					367:378	simultaneous laser vaporization	348:378	simultaneous laser vaporization of the two distinct material targets	348:415	Compositional gradients were obtained by simultaneous laser vaporization of the two distinct material targets.
27418570	10	74	dep	best	1446:1449	arg1	compromise					1451:1460	compromise	1451:1460	compromise	1451:1460	The best compromise should therefore go, in our opinion, to zones with intermediate-to-high chitosan concentration which can assure a large spectrum of antimicrobial protection concomitantly with a significant enhancement of osseointegration, favored by the presence of biomimetic hydroxyapatite.
27418570	4	75	theme	deposited	535:543	arg1	materials					545:553	deposited materials	535:553	deposited materials	535:553	The nature and surface composition of deposited materials and the spatial distribution of constituents were studied by SEM, EDS, AFM, GIXRD, FTIR, micro-Raman, and XPS.
27418570	10	76	theme	chitosan	1534:1541	arg1	concentration					1543:1555	intermediate-to-high chitosan concentration	1513:1555	intermediate-to-high chitosan concentration which can assure a large spectrum of antimicrobial protection concomitantly with a significant enhancement of osseointegration, favored by the presence of biomimetic hydroxyapatite	1513:1736	The best compromise should therefore go, in our opinion, to zones with intermediate-to-high chitosan concentration which can assure a large spectrum of antimicrobial protection concomitantly with a significant enhancement of osseointegration, favored by the presence of biomimetic hydroxyapatite.
27418570	2	77	theme	targets	409:415	arg1	vaporization					367:378	simultaneous laser vaporization	348:378	simultaneous laser vaporization of the two distinct material targets	348:415	Compositional gradients were obtained by simultaneous laser vaporization of the two distinct material targets.
27418570	3	78	theme	KrF	420:422	arg1	source					461:466	A KrF* excimer (λ=248nm, τFWHM=25ns) laser source	418:466	source	461:466	A KrF* excimer (λ=248nm, τFWHM=25ns) laser source was used in all experiments.
27418570	8	79	theme	C-MAPLE	1157:1163	arg1	films					1175:1179	the BmAp-CHT C-MAPLE composite films	1144:1179	the BmAp-CHT C-MAPLE composite films	1144:1179	FTIR, EDS and XPS analyses indicated that the composition of the BmAp-CHT C-MAPLE composite films gradually modified from pure apatite to chitosan.
27418570	4	80	theme	surface	512:518	arg1	composition					520:530	surface composition	512:530	surface composition	512:530	The nature and surface composition of deposited materials and the spatial distribution of constituents were studied by SEM, EDS, AFM, GIXRD, FTIR, micro-Raman, and XPS.
27418570	10	81	theme	significant	1640:1650	arg1	enhancement					1652:1662	a significant enhancement	1638:1662	a significant enhancement	1638:1662	The best compromise should therefore go, in our opinion, to zones with intermediate-to-high chitosan concentration which can assure a large spectrum of antimicrobial protection concomitantly with a significant enhancement of osseointegration, favored by the presence of biomimetic hydroxyapatite.
27418570	2	82	theme	material	400:407	arg1	targets					409:415	the two distinct material targets	383:415	the two distinct material targets	383:415	Compositional gradients were obtained by simultaneous laser vaporization of the two distinct material targets.
27418570	10	83	theme	osseointegration	1667:1682	arg1	enhancement					1652:1662	a significant enhancement	1638:1662	a significant enhancement	1638:1662	The best compromise should therefore go, in our opinion, to zones with intermediate-to-high chitosan concentration which can assure a large spectrum of antimicrobial protection concomitantly with a significant enhancement of osseointegration, favored by the presence of biomimetic hydroxyapatite.
27418570	3	84	used	used	472:475	arg2	source					461:466	A KrF* excimer (λ=248nm, τFWHM=25ns) laser source	418:466	source	461:466	A KrF* excimer (λ=248nm, τFWHM=25ns) laser source was used in all experiments.
25909987	4	0	theme	ionization	818:827	arg1	Py-FIMS					848:854	Py-FIMS	848:854	Py-FIMS	848:854	Chemical analyses comprised isotopic composition of C and N, cellulosic (CPS), non-cellulosic (NCPS), and N-containing saccharides, and molecular composition screening using pyrolysis-field ionization mass spectrometry (Py-FIMS).
25909987	4	0	theme	ionization	818:827	arg1	spectrometry					834:845	pyrolysis-field ionization mass spectrometry	802:845	pyrolysis-field ionization mass spectrometry (Py-FIMS)	802:855	Chemical analyses comprised isotopic composition of C and N, cellulosic (CPS), non-cellulosic (NCPS), and N-containing saccharides, and molecular composition screening using pyrolysis-field ionization mass spectrometry (Py-FIMS).
25909987	9	1	theme	modification	1829:1840	arg1	evidence					1817:1824	evidence	1817:1824	evidence of modification by microbial processes	1817:1863	We therewith document that nest chemistry of rainforest termites shows variations and evidence of modification by microbial processes, but nevertheless it primarily reflects the trophic niches of the constructors.
25909987	9	1	theme	modification	1829:1840	arg1	variations					1802:1811	variations	1802:1811	variations	1802:1811	We therewith document that nest chemistry of rainforest termites shows variations and evidence of modification by microbial processes, but nevertheless it primarily reflects the trophic niches of the constructors.
25909987	9	2	theme	microbial	1845:1853	arg1	processes					1855:1863	microbial processes	1845:1863	microbial processes	1845:1863	We therewith document that nest chemistry of rainforest termites shows variations and evidence of modification by microbial processes, but nevertheless it primarily reflects the trophic niches of the constructors.
25909987	6	3	theme	elevated	1176:1183	arg1	NCPS					1185:1188	elevated NCPS	1176:1188	elevated NCPS	1176:1188	This result was confirmed by elevated NCPS:CPS ratios, indicating a preferential cellulose decomposition in the nests.
25909987	2	4	theme	food	277:280	arg1	sources					282:288	food sources	277:288	food sources	277:288	The identification of food sources, however, is difficult, because they are variable and changed by termite activity and nest construction.
25909987	7	5	theme	transformation	1352:1365	arg1	processes					1367:1375	the transformation processes	1348:1375	the transformation processes	1348:1375	High portions of muramic acid (MurAc) pointed to the participation of bacteria in the transformation processes.
25909987	4	6	theme	composition	774:784	arg1	screening					786:794	molecular composition screening	764:794	molecular composition screening using pyrolysis-field ionization mass spectrometry (Py-FIMS)	764:855	Chemical analyses comprised isotopic composition of C and N, cellulosic (CPS), non-cellulosic (NCPS), and N-containing saccharides, and molecular composition screening using pyrolysis-field ionization mass spectrometry (Py-FIMS).
25909987	4	7	theme	Chemical	628:635	arg1	analyses					637:644	Chemical analyses	628:644	Chemical analyses	628:644	Chemical analyses comprised isotopic composition of C and N, cellulosic (CPS), non-cellulosic (NCPS), and N-containing saccharides, and molecular composition screening using pyrolysis-field ionization mass spectrometry (Py-FIMS).
25909987	5	8	with	enrichment	1069:1078	arg1	alterations					1100:1110	organic matter alterations	1085:1110	organic matter alterations during or after nest construction	1085:1144	The isotopic analysis revealed higher soil δ13C (-27.4‰) and δ15N (6.6‰) values in nests of wood feeding Nasutitermes and Cornitermes than in wood samples (δ13C = -29.1‰, δ15N = 3.4‰), reflecting stable-isotope enrichment with organic matter alterations during or after nest construction.
25909987	3	9	theme	Firme	534:538	arg1	rainforests					540:550	the Terra Firme rainforests	524:550	the Terra Firme rainforests	524:550	Here, we related the sources and alteration of organic matter in nests from seven different termite genera and feeding habits in the Terra Firme rainforests to the properties of potential food sources soil, wood, and microepiphytes.
25909987	1	10	theme	soil	171:174	arg1	formation					176:184	tropical soil formation	162:184	tropical soil formation	162:184	The impact of termites on nutrient cycling and tropical soil formation depends on their feeding habits and related material transformation.
25909987	0	11	theme	rainforests	102:112	arg1	guilds					81:86	different feeding guilds	63:86	different feeding guilds of the Amazon rainforests	63:112	Origin and alteration of organic matter in termite mounds from different feeding guilds of the Amazon rainforests.
25909987	7	12	theme	muramic	1283:1289	arg1	MurAc					1297:1301	MurAc	1297:1301	MurAc	1297:1301	High portions of muramic acid (MurAc) pointed to the participation of bacteria in the transformation processes.
25909987	7	12	theme	muramic	1283:1289	arg1	acid					1291:1294	muramic acid	1283:1294	muramic acid (MurAc)	1283:1302	High portions of muramic acid (MurAc) pointed to the participation of bacteria in the transformation processes.
25909987	6	13	theme	preferential	1215:1226	arg1	decomposition					1238:1250	a preferential cellulose decomposition	1213:1250	a preferential cellulose decomposition in the nests	1213:1263	This result was confirmed by elevated NCPS:CPS ratios, indicating a preferential cellulose decomposition in the nests.
25909987	5	14	theme	wood	1000:1003	arg1	3.4‰					1036:1039	δ13C = -29.1‰, δ15N = 3.4‰	1014:1039	3.4‰	1036:1039	The isotopic analysis revealed higher soil δ13C (-27.4‰) and δ15N (6.6‰) values in nests of wood feeding Nasutitermes and Cornitermes than in wood samples (δ13C = -29.1‰, δ15N = 3.4‰), reflecting stable-isotope enrichment with organic matter alterations during or after nest construction.
25909987	5	14	theme	wood	1000:1003	arg1	samples					1005:1011	wood samples	1000:1011	wood samples (δ13C = -29.1‰, δ15N = 3.4‰)	1000:1040	The isotopic analysis revealed higher soil δ13C (-27.4‰) and δ15N (6.6‰) values in nests of wood feeding Nasutitermes and Cornitermes than in wood samples (δ13C = -29.1‰, δ15N = 3.4‰), reflecting stable-isotope enrichment with organic matter alterations during or after nest construction.
25909987	3	15	dep	sources	588:594	arg1	wood					602:605	wood	602:605	wood	602:605	Here, we related the sources and alteration of organic matter in nests from seven different termite genera and feeding habits in the Terra Firme rainforests to the properties of potential food sources soil, wood, and microepiphytes.
25909987	3	15	dep	sources	588:594	arg1	microepiphytes					612:625	microepiphytes	612:625	microepiphytes	612:625	Here, we related the sources and alteration of organic matter in nests from seven different termite genera and feeding habits in the Terra Firme rainforests to the properties of potential food sources soil, wood, and microepiphytes.
25909987	3	15	dep	sources	588:594	arg1	soil					596:599	soil	596:599	soil	596:599	Here, we related the sources and alteration of organic matter in nests from seven different termite genera and feeding habits in the Terra Firme rainforests to the properties of potential food sources soil, wood, and microepiphytes.
25909987	3	15	dep	sources	588:594	arg1	sources					588:594	potential food sources soil, wood, and microepiphytes	573:625	potential food sources soil, wood, and microepiphytes	573:625	Here, we related the sources and alteration of organic matter in nests from seven different termite genera and feeding habits in the Terra Firme rainforests to the properties of potential food sources soil, wood, and microepiphytes.
25909987	4	16	theme	C	680:680	arg1	screening					786:794	molecular composition screening	764:794	molecular composition screening using pyrolysis-field ionization mass spectrometry (Py-FIMS)	764:855	Chemical analyses comprised isotopic composition of C and N, cellulosic (CPS), non-cellulosic (NCPS), and N-containing saccharides, and molecular composition screening using pyrolysis-field ionization mass spectrometry (Py-FIMS).
25909987	4	16	theme	C	680:680	arg1	saccharides					747:757	cellulosic (CPS), non-cellulosic (NCPS), and N-containing saccharides	689:757	cellulosic (CPS), non-cellulosic (NCPS), and N-containing saccharides	689:757	Chemical analyses comprised isotopic composition of C and N, cellulosic (CPS), non-cellulosic (NCPS), and N-containing saccharides, and molecular composition screening using pyrolysis-field ionization mass spectrometry (Py-FIMS).
25909987	4	16	theme	C	680:680	arg1	composition					665:675	isotopic composition	656:675	isotopic composition of C and N	656:686	Chemical analyses comprised isotopic composition of C and N, cellulosic (CPS), non-cellulosic (NCPS), and N-containing saccharides, and molecular composition screening using pyrolysis-field ionization mass spectrometry (Py-FIMS).
25909987	9	17	dep	We	1731:1732	arg1	therewith					1734:1742	therewith	1734:1742	We therewith	1731:1742	We therewith document that nest chemistry of rainforest termites shows variations and evidence of modification by microbial processes, but nevertheless it primarily reflects the trophic niches of the constructors.
25909987	5	18	theme	feeding	955:961	arg1	nests					941:945	nests	941:945	nests of wood feeding	941:961	The isotopic analysis revealed higher soil δ13C (-27.4‰) and δ15N (6.6‰) values in nests of wood feeding Nasutitermes and Cornitermes than in wood samples (δ13C = -29.1‰, δ15N = 3.4‰), reflecting stable-isotope enrichment with organic matter alterations during or after nest construction.
25909987	5	19	theme	isotopic	862:869	arg1	analysis					871:878	The isotopic analysis	858:878	The isotopic analysis	858:878	The isotopic analysis revealed higher soil δ13C (-27.4‰) and δ15N (6.6‰) values in nests of wood feeding Nasutitermes and Cornitermes than in wood samples (δ13C = -29.1‰, δ15N = 3.4‰), reflecting stable-isotope enrichment with organic matter alterations during or after nest construction.
25909987	4	20	theme	N	686:686	arg1	screening					786:794	molecular composition screening	764:794	molecular composition screening using pyrolysis-field ionization mass spectrometry (Py-FIMS)	764:855	Chemical analyses comprised isotopic composition of C and N, cellulosic (CPS), non-cellulosic (NCPS), and N-containing saccharides, and molecular composition screening using pyrolysis-field ionization mass spectrometry (Py-FIMS).
25909987	4	20	theme	N	686:686	arg1	saccharides					747:757	cellulosic (CPS), non-cellulosic (NCPS), and N-containing saccharides	689:757	cellulosic (CPS), non-cellulosic (NCPS), and N-containing saccharides	689:757	Chemical analyses comprised isotopic composition of C and N, cellulosic (CPS), non-cellulosic (NCPS), and N-containing saccharides, and molecular composition screening using pyrolysis-field ionization mass spectrometry (Py-FIMS).
25909987	4	20	theme	N	686:686	arg1	composition					665:675	isotopic composition	656:675	isotopic composition of C and N	656:686	Chemical analyses comprised isotopic composition of C and N, cellulosic (CPS), non-cellulosic (NCPS), and N-containing saccharides, and molecular composition screening using pyrolysis-field ionization mass spectrometry (Py-FIMS).
25909987	3	21	theme	sources	588:594	arg1	properties					559:568	the properties	555:568	the properties of potential food sources soil, wood, and microepiphytes	555:625	Here, we related the sources and alteration of organic matter in nests from seven different termite genera and feeding habits in the Terra Firme rainforests to the properties of potential food sources soil, wood, and microepiphytes.
25909987	0	22	from	guilds	81:86	arg1	alteration					11:20	alteration	11:20	alteration	11:20	Origin and alteration of organic matter in termite mounds from different feeding guilds of the Amazon rainforests.
25909987	0	22	from	guilds	81:86	arg1	Origin					0:5	Origin	0:5	Origin	0:5	Origin and alteration of organic matter in termite mounds from different feeding guilds of the Amazon rainforests.
25909987	0	22	from	guilds	81:86	arg1	mounds					51:56	termite mounds	43:56	termite mounds from different feeding guilds of the Amazon rainforests	43:112	Origin and alteration of organic matter in termite mounds from different feeding guilds of the Amazon rainforests.
25909987	5	23	dep	δ13C	901:904	arg1	Cornitermes					980:990	Cornitermes	980:990	Cornitermes	980:990	The isotopic analysis revealed higher soil δ13C (-27.4‰) and δ15N (6.6‰) values in nests of wood feeding Nasutitermes and Cornitermes than in wood samples (δ13C = -29.1‰, δ15N = 3.4‰), reflecting stable-isotope enrichment with organic matter alterations during or after nest construction.
25909987	5	23	dep	δ13C	901:904	arg1	Nasutitermes					963:974	Nasutitermes	963:974	Nasutitermes	963:974	The isotopic analysis revealed higher soil δ13C (-27.4‰) and δ15N (6.6‰) values in nests of wood feeding Nasutitermes and Cornitermes than in wood samples (δ13C = -29.1‰, δ15N = 3.4‰), reflecting stable-isotope enrichment with organic matter alterations during or after nest construction.
25909987	3	24	theme	feeding	506:512	arg1	habits					514:519	feeding habits	506:519	feeding habits	506:519	Here, we related the sources and alteration of organic matter in nests from seven different termite genera and feeding habits in the Terra Firme rainforests to the properties of potential food sources soil, wood, and microepiphytes.
25909987	3	25	theme	potential	573:581	arg1	wood					602:605	wood	602:605	wood	602:605	Here, we related the sources and alteration of organic matter in nests from seven different termite genera and feeding habits in the Terra Firme rainforests to the properties of potential food sources soil, wood, and microepiphytes.
25909987	3	25	theme	potential	573:581	arg1	microepiphytes					612:625	microepiphytes	612:625	microepiphytes	612:625	Here, we related the sources and alteration of organic matter in nests from seven different termite genera and feeding habits in the Terra Firme rainforests to the properties of potential food sources soil, wood, and microepiphytes.
25909987	3	25	theme	potential	573:581	arg1	soil					596:599	soil	596:599	soil	596:599	Here, we related the sources and alteration of organic matter in nests from seven different termite genera and feeding habits in the Terra Firme rainforests to the properties of potential food sources soil, wood, and microepiphytes.
25909987	3	25	theme	potential	573:581	arg1	sources					588:594	potential food sources soil, wood, and microepiphytes	573:625	potential food sources soil, wood, and microepiphytes	573:625	Here, we related the sources and alteration of organic matter in nests from seven different termite genera and feeding habits in the Terra Firme rainforests to the properties of potential food sources soil, wood, and microepiphytes.
25909987	4	26	dep	non-cellulosic	707:720	arg1	NCPS					723:726	NCPS	723:726	NCPS	723:726	Chemical analyses comprised isotopic composition of C and N, cellulosic (CPS), non-cellulosic (NCPS), and N-containing saccharides, and molecular composition screening using pyrolysis-field ionization mass spectrometry (Py-FIMS).
25909987	0	27	from	alteration	11:20	arg1	mounds					51:56	termite mounds	43:56	termite mounds from different feeding guilds of the Amazon rainforests	43:112	Origin and alteration of organic matter in termite mounds from different feeding guilds of the Amazon rainforests.
25909987	0	27	from	alteration	11:20	arg1	guilds					81:86	different feeding guilds	63:86	different feeding guilds of the Amazon rainforests	63:112	Origin and alteration of organic matter in termite mounds from different feeding guilds of the Amazon rainforests.
25909987	0	28	theme	different	63:71	arg1	guilds					81:86	different feeding guilds	63:86	different feeding guilds of the Amazon rainforests	63:112	Origin and alteration of organic matter in termite mounds from different feeding guilds of the Amazon rainforests.
25909987	5	29	theme	=	1034:1034	arg1	samples					1005:1011	wood samples	1000:1011	wood samples (δ13C = -29.1‰, δ15N = 3.4‰)	1000:1040	The isotopic analysis revealed higher soil δ13C (-27.4‰) and δ15N (6.6‰) values in nests of wood feeding Nasutitermes and Cornitermes than in wood samples (δ13C = -29.1‰, δ15N = 3.4‰), reflecting stable-isotope enrichment with organic matter alterations during or after nest construction.
25909987	5	29	theme	=	1034:1034	arg1	3.4‰					1036:1039	δ13C = -29.1‰, δ15N = 3.4‰	1014:1039	3.4‰	1036:1039	The isotopic analysis revealed higher soil δ13C (-27.4‰) and δ15N (6.6‰) values in nests of wood feeding Nasutitermes and Cornitermes than in wood samples (δ13C = -29.1‰, δ15N = 3.4‰), reflecting stable-isotope enrichment with organic matter alterations during or after nest construction.
25909987	1	30	theme	nutrient	141:148	arg1	cycling					150:156	nutrient cycling	141:156	nutrient cycling	141:156	The impact of termites on nutrient cycling and tropical soil formation depends on their feeding habits and related material transformation.
25909987	7	31	from	participation	1319:1331	arg1	processes					1367:1375	the transformation processes	1348:1375	the transformation processes	1348:1375	High portions of muramic acid (MurAc) pointed to the participation of bacteria in the transformation processes.
25909987	0	32	from	Origin	0:5	arg1	mounds					51:56	termite mounds	43:56	termite mounds from different feeding guilds of the Amazon rainforests	43:112	Origin and alteration of organic matter in termite mounds from different feeding guilds of the Amazon rainforests.
25909987	0	32	from	Origin	0:5	arg1	guilds					81:86	different feeding guilds	63:86	different feeding guilds of the Amazon rainforests	63:112	Origin and alteration of organic matter in termite mounds from different feeding guilds of the Amazon rainforests.
25909987	3	33	from	habits	514:519	arg1	rainforests					540:550	the Terra Firme rainforests	524:550	the Terra Firme rainforests	524:550	Here, we related the sources and alteration of organic matter in nests from seven different termite genera and feeding habits in the Terra Firme rainforests to the properties of potential food sources soil, wood, and microepiphytes.
25909987	3	33	from	habits	514:519	arg1	nests					460:464	nests	460:464	nests from seven different termite genera and feeding habits in the Terra Firme rainforests	460:550	Here, we related the sources and alteration of organic matter in nests from seven different termite genera and feeding habits in the Terra Firme rainforests to the properties of potential food sources soil, wood, and microepiphytes.
25909987	3	33	from	habits	514:519	arg1	alteration					428:437	alteration	428:437	alteration of organic matter in nests from seven different termite genera and feeding habits in the Terra Firme rainforests	428:550	Here, we related the sources and alteration of organic matter in nests from seven different termite genera and feeding habits in the Terra Firme rainforests to the properties of potential food sources soil, wood, and microepiphytes.
25909987	3	33	from	habits	514:519	arg1	sources					416:422	the sources	412:422	the sources	412:422	Here, we related the sources and alteration of organic matter in nests from seven different termite genera and feeding habits in the Terra Firme rainforests to the properties of potential food sources soil, wood, and microepiphytes.
25909987	5	34	theme	=	1019:1019	arg1	samples					1005:1011	wood samples	1000:1011	wood samples (δ13C = -29.1‰, δ15N = 3.4‰)	1000:1040	The isotopic analysis revealed higher soil δ13C (-27.4‰) and δ15N (6.6‰) values in nests of wood feeding Nasutitermes and Cornitermes than in wood samples (δ13C = -29.1‰, δ15N = 3.4‰), reflecting stable-isotope enrichment with organic matter alterations during or after nest construction.
25909987	5	34	theme	=	1019:1019	arg1	3.4‰					1036:1039	δ13C = -29.1‰, δ15N = 3.4‰	1014:1039	3.4‰	1036:1039	The isotopic analysis revealed higher soil δ13C (-27.4‰) and δ15N (6.6‰) values in nests of wood feeding Nasutitermes and Cornitermes than in wood samples (δ13C = -29.1‰, δ15N = 3.4‰), reflecting stable-isotope enrichment with organic matter alterations during or after nest construction.
25909987	5	35	theme	δ15N	919:922	arg1	values					931:936	higher soil δ13C (-27.4‰) and δ15N (6.6‰) values	889:936	values	931:936	The isotopic analysis revealed higher soil δ13C (-27.4‰) and δ15N (6.6‰) values in nests of wood feeding Nasutitermes and Cornitermes than in wood samples (δ13C = -29.1‰, δ15N = 3.4‰), reflecting stable-isotope enrichment with organic matter alterations during or after nest construction.
25909987	5	35	theme	δ15N	919:922	arg1	6.6‰					925:928	6.6‰	925:928	6.6‰	925:928	The isotopic analysis revealed higher soil δ13C (-27.4‰) and δ15N (6.6‰) values in nests of wood feeding Nasutitermes and Cornitermes than in wood samples (δ13C = -29.1‰, δ15N = 3.4‰), reflecting stable-isotope enrichment with organic matter alterations during or after nest construction.
25909987	5	36	theme	matter	1093:1098	arg1	alterations					1100:1110	organic matter alterations	1085:1110	organic matter alterations during or after nest construction	1085:1144	The isotopic analysis revealed higher soil δ13C (-27.4‰) and δ15N (6.6‰) values in nests of wood feeding Nasutitermes and Cornitermes than in wood samples (δ13C = -29.1‰, δ15N = 3.4‰), reflecting stable-isotope enrichment with organic matter alterations during or after nest construction.
25909987	4	37	theme	cellulosic	689:698	arg1	saccharides					747:757	cellulosic (CPS), non-cellulosic (NCPS), and N-containing saccharides	689:757	cellulosic (CPS), non-cellulosic (NCPS), and N-containing saccharides	689:757	Chemical analyses comprised isotopic composition of C and N, cellulosic (CPS), non-cellulosic (NCPS), and N-containing saccharides, and molecular composition screening using pyrolysis-field ionization mass spectrometry (Py-FIMS).
25909987	2	38	theme	termite	355:361	arg1	activity					363:370	termite activity	355:370	termite activity	355:370	The identification of food sources, however, is difficult, because they are variable and changed by termite activity and nest construction.
25909987	8	39	theme	Non-metric	1378:1387	arg1	MDS					1415:1417	MDS	1415:1417	MDS	1415:1417	Non-metric multidimensional scaling (MDS) revealed increasing geophagy in the sequence Termes < Embiratermes < Anoplotermes and increasing xylophagy for Cornitermes < Nasutitermes., and that the nest material of Constrictotermes was similar to the microepiphytes sample, confirming the report that Constrictotermes belongs to the microepiphyte-feeders.
25909987	8	39	theme	Non-metric	1378:1387	arg1	scaling					1406:1412	Non-metric multidimensional scaling	1378:1412	Non-metric multidimensional scaling (MDS)	1378:1418	Non-metric multidimensional scaling (MDS) revealed increasing geophagy in the sequence Termes < Embiratermes < Anoplotermes and increasing xylophagy for Cornitermes < Nasutitermes., and that the nest material of Constrictotermes was similar to the microepiphytes sample, confirming the report that Constrictotermes belongs to the microepiphyte-feeders.
25909987	5	40	theme	soil	896:899	arg1	δ13C					901:904	higher soil δ13C (-27.4‰) and δ15N (6.6‰) values	889:936	δ13C	901:904	The isotopic analysis revealed higher soil δ13C (-27.4‰) and δ15N (6.6‰) values in nests of wood feeding Nasutitermes and Cornitermes than in wood samples (δ13C = -29.1‰, δ15N = 3.4‰), reflecting stable-isotope enrichment with organic matter alterations during or after nest construction.
25909987	5	40	theme	soil	896:899	arg1	-27.4‰					907:912	-27.4‰	907:912	-27.4‰	907:912	The isotopic analysis revealed higher soil δ13C (-27.4‰) and δ15N (6.6‰) values in nests of wood feeding Nasutitermes and Cornitermes than in wood samples (δ13C = -29.1‰, δ15N = 3.4‰), reflecting stable-isotope enrichment with organic matter alterations during or after nest construction.
25909987	5	41	theme	organic	1085:1091	arg1	alterations					1100:1110	organic matter alterations	1085:1110	organic matter alterations during or after nest construction	1085:1144	The isotopic analysis revealed higher soil δ13C (-27.4‰) and δ15N (6.6‰) values in nests of wood feeding Nasutitermes and Cornitermes than in wood samples (δ13C = -29.1‰, δ15N = 3.4‰), reflecting stable-isotope enrichment with organic matter alterations during or after nest construction.
25909987	0	42	theme	organic	25:31	arg1	matter					33:38	organic matter	25:38	organic matter	25:38	Origin and alteration of organic matter in termite mounds from different feeding guilds of the Amazon rainforests.
25909987	8	43	from	geophagy	1440:1447	arg1	Anoplotermes					1489:1500	Anoplotermes	1489:1500	Anoplotermes	1489:1500	Non-metric multidimensional scaling (MDS) revealed increasing geophagy in the sequence Termes < Embiratermes < Anoplotermes and increasing xylophagy for Cornitermes < Nasutitermes., and that the nest material of Constrictotermes was similar to the microepiphytes sample, confirming the report that Constrictotermes belongs to the microepiphyte-feeders.
25909987	9	44	theme	rainforest	1776:1785	arg1	termites					1787:1794	rainforest termites	1776:1794	rainforest termites	1776:1794	We therewith document that nest chemistry of rainforest termites shows variations and evidence of modification by microbial processes, but nevertheless it primarily reflects the trophic niches of the constructors.
25909987	5	45	theme	wood	950:953	arg1	feeding					955:961	wood feeding	950:961	wood feeding	950:961	The isotopic analysis revealed higher soil δ13C (-27.4‰) and δ15N (6.6‰) values in nests of wood feeding Nasutitermes and Cornitermes than in wood samples (δ13C = -29.1‰, δ15N = 3.4‰), reflecting stable-isotope enrichment with organic matter alterations during or after nest construction.
25909987	3	46	theme	organic	442:448	arg1	matter					450:455	organic matter	442:455	organic matter	442:455	Here, we related the sources and alteration of organic matter in nests from seven different termite genera and feeding habits in the Terra Firme rainforests to the properties of potential food sources soil, wood, and microepiphytes.
25909987	5	47	theme	nest	1128:1131	arg1	construction					1133:1144	nest construction	1128:1144	nest construction	1128:1144	The isotopic analysis revealed higher soil δ13C (-27.4‰) and δ15N (6.6‰) values in nests of wood feeding Nasutitermes and Cornitermes than in wood samples (δ13C = -29.1‰, δ15N = 3.4‰), reflecting stable-isotope enrichment with organic matter alterations during or after nest construction.
25909987	1	48	theme	termites	129:136	arg1	impact					119:124	The impact	115:124	The impact of termites on nutrient cycling and tropical soil formation	115:184	The impact of termites on nutrient cycling and tropical soil formation depends on their feeding habits and related material transformation.
25909987	3	49	theme	different	477:485	arg1	genera					495:500	seven different termite genera	471:500	seven different termite genera	471:500	Here, we related the sources and alteration of organic matter in nests from seven different termite genera and feeding habits in the Terra Firme rainforests to the properties of potential food sources soil, wood, and microepiphytes.
25909987	8	50	theme	nest	1573:1576	arg1	material					1578:1585	the nest material	1569:1585	the nest material of Constrictotermes	1569:1605	Non-metric multidimensional scaling (MDS) revealed increasing geophagy in the sequence Termes < Embiratermes < Anoplotermes and increasing xylophagy for Cornitermes < Nasutitermes., and that the nest material of Constrictotermes was similar to the microepiphytes sample, confirming the report that Constrictotermes belongs to the microepiphyte-feeders.
25909987	8	50	theme	nest	1573:1576	arg1	similar					1611:1617	similar	1611:1617	similar	1611:1617	Non-metric multidimensional scaling (MDS) revealed increasing geophagy in the sequence Termes < Embiratermes < Anoplotermes and increasing xylophagy for Cornitermes < Nasutitermes., and that the nest material of Constrictotermes was similar to the microepiphytes sample, confirming the report that Constrictotermes belongs to the microepiphyte-feeders.
25909987	6	51	theme	CPS	1190:1192	arg1	ratios					1194:1199	CPS ratios	1190:1199	CPS ratios	1190:1199	This result was confirmed by elevated NCPS:CPS ratios, indicating a preferential cellulose decomposition in the nests.
25909987	1	52	theme	material	230:237	arg1	transformation					239:252	related material transformation	222:252	related material transformation	222:252	The impact of termites on nutrient cycling and tropical soil formation depends on their feeding habits and related material transformation.
25909987	4	53	theme	pyrolysis-field	802:816	arg1	Py-FIMS					848:854	Py-FIMS	848:854	Py-FIMS	848:854	Chemical analyses comprised isotopic composition of C and N, cellulosic (CPS), non-cellulosic (NCPS), and N-containing saccharides, and molecular composition screening using pyrolysis-field ionization mass spectrometry (Py-FIMS).
25909987	4	53	theme	pyrolysis-field	802:816	arg1	spectrometry					834:845	pyrolysis-field ionization mass spectrometry	802:845	pyrolysis-field ionization mass spectrometry (Py-FIMS)	802:855	Chemical analyses comprised isotopic composition of C and N, cellulosic (CPS), non-cellulosic (NCPS), and N-containing saccharides, and molecular composition screening using pyrolysis-field ionization mass spectrometry (Py-FIMS).
25909987	2	54	theme	sources	282:288	arg1	identification					259:272	The identification	255:272	The identification of food sources	255:288	The identification of food sources, however, is difficult, because they are variable and changed by termite activity and nest construction.
25909987	2	54	theme	sources	282:288	arg1	difficult					303:311	difficult	303:311	difficult	303:311	The identification of food sources, however, is difficult, because they are variable and changed by termite activity and nest construction.
25909987	4	55	theme	mass	829:832	arg1	Py-FIMS					848:854	Py-FIMS	848:854	Py-FIMS	848:854	Chemical analyses comprised isotopic composition of C and N, cellulosic (CPS), non-cellulosic (NCPS), and N-containing saccharides, and molecular composition screening using pyrolysis-field ionization mass spectrometry (Py-FIMS).
25909987	4	55	theme	mass	829:832	arg1	spectrometry					834:845	pyrolysis-field ionization mass spectrometry	802:845	pyrolysis-field ionization mass spectrometry (Py-FIMS)	802:855	Chemical analyses comprised isotopic composition of C and N, cellulosic (CPS), non-cellulosic (NCPS), and N-containing saccharides, and molecular composition screening using pyrolysis-field ionization mass spectrometry (Py-FIMS).
25909987	5	56	from	values	931:936	arg1	nests					941:945	nests	941:945	nests of wood feeding	941:961	The isotopic analysis revealed higher soil δ13C (-27.4‰) and δ15N (6.6‰) values in nests of wood feeding Nasutitermes and Cornitermes than in wood samples (δ13C = -29.1‰, δ15N = 3.4‰), reflecting stable-isotope enrichment with organic matter alterations during or after nest construction.
25909987	7	57	theme	bacteria	1336:1343	arg1	participation					1319:1331	the participation	1315:1331	the participation of bacteria in the transformation processes	1315:1375	High portions of muramic acid (MurAc) pointed to the participation of bacteria in the transformation processes.
25909987	4	58	theme	molecular	764:772	arg1	screening					786:794	molecular composition screening	764:794	molecular composition screening using pyrolysis-field ionization mass spectrometry (Py-FIMS)	764:855	Chemical analyses comprised isotopic composition of C and N, cellulosic (CPS), non-cellulosic (NCPS), and N-containing saccharides, and molecular composition screening using pyrolysis-field ionization mass spectrometry (Py-FIMS).
25909987	3	59	theme	Terra	528:532	arg1	rainforests					540:550	the Terra Firme rainforests	524:550	the Terra Firme rainforests	524:550	Here, we related the sources and alteration of organic matter in nests from seven different termite genera and feeding habits in the Terra Firme rainforests to the properties of potential food sources soil, wood, and microepiphytes.
25909987	8	60	theme	microepiphytes	1626:1639	arg1	sample					1641:1646	the microepiphytes sample	1622:1646	the microepiphytes sample	1622:1646	Non-metric multidimensional scaling (MDS) revealed increasing geophagy in the sequence Termes < Embiratermes < Anoplotermes and increasing xylophagy for Cornitermes < Nasutitermes., and that the nest material of Constrictotermes was similar to the microepiphytes sample, confirming the report that Constrictotermes belongs to the microepiphyte-feeders.
25909987	1	61	theme	tropical	162:169	arg1	formation					176:184	tropical soil formation	162:184	tropical soil formation	162:184	The impact of termites on nutrient cycling and tropical soil formation depends on their feeding habits and related material transformation.
25909987	6	62	theme	cellulose	1228:1236	arg1	decomposition					1238:1250	a preferential cellulose decomposition	1213:1250	a preferential cellulose decomposition in the nests	1213:1263	This result was confirmed by elevated NCPS:CPS ratios, indicating a preferential cellulose decomposition in the nests.
25909987	7	63	theme	acid	1291:1294	arg1	portions					1271:1278	High portions	1266:1278	High portions of muramic acid (MurAc)	1266:1302	High portions of muramic acid (MurAc) pointed to the participation of bacteria in the transformation processes.
25909987	9	64	theme	trophic	1909:1915	arg1	niches					1917:1922	the trophic niches	1905:1922	the trophic niches of the constructors	1905:1942	We therewith document that nest chemistry of rainforest termites shows variations and evidence of modification by microbial processes, but nevertheless it primarily reflects the trophic niches of the constructors.
25909987	3	65	theme	food	583:586	arg1	wood					602:605	wood	602:605	wood	602:605	Here, we related the sources and alteration of organic matter in nests from seven different termite genera and feeding habits in the Terra Firme rainforests to the properties of potential food sources soil, wood, and microepiphytes.
25909987	3	65	theme	food	583:586	arg1	microepiphytes					612:625	microepiphytes	612:625	microepiphytes	612:625	Here, we related the sources and alteration of organic matter in nests from seven different termite genera and feeding habits in the Terra Firme rainforests to the properties of potential food sources soil, wood, and microepiphytes.
25909987	3	65	theme	food	583:586	arg1	soil					596:599	soil	596:599	soil	596:599	Here, we related the sources and alteration of organic matter in nests from seven different termite genera and feeding habits in the Terra Firme rainforests to the properties of potential food sources soil, wood, and microepiphytes.
25909987	3	65	theme	food	583:586	arg1	sources					588:594	potential food sources soil, wood, and microepiphytes	573:625	potential food sources soil, wood, and microepiphytes	573:625	Here, we related the sources and alteration of organic matter in nests from seven different termite genera and feeding habits in the Terra Firme rainforests to the properties of potential food sources soil, wood, and microepiphytes.
25909987	4	66	dep	cellulosic	689:698	arg1	CPS					701:703	CPS	701:703	CPS	701:703	Chemical analyses comprised isotopic composition of C and N, cellulosic (CPS), non-cellulosic (NCPS), and N-containing saccharides, and molecular composition screening using pyrolysis-field ionization mass spectrometry (Py-FIMS).
25909987	8	67	theme	multidimensional	1389:1404	arg1	MDS					1415:1417	MDS	1415:1417	MDS	1415:1417	Non-metric multidimensional scaling (MDS) revealed increasing geophagy in the sequence Termes < Embiratermes < Anoplotermes and increasing xylophagy for Cornitermes < Nasutitermes., and that the nest material of Constrictotermes was similar to the microepiphytes sample, confirming the report that Constrictotermes belongs to the microepiphyte-feeders.
25909987	8	67	theme	multidimensional	1389:1404	arg1	scaling					1406:1412	Non-metric multidimensional scaling	1378:1412	Non-metric multidimensional scaling (MDS)	1378:1418	Non-metric multidimensional scaling (MDS) revealed increasing geophagy in the sequence Termes < Embiratermes < Anoplotermes and increasing xylophagy for Cornitermes < Nasutitermes., and that the nest material of Constrictotermes was similar to the microepiphytes sample, confirming the report that Constrictotermes belongs to the microepiphyte-feeders.
25909987	7	68	theme	High	1266:1269	arg1	portions					1271:1278	High portions	1266:1278	High portions of muramic acid (MurAc)	1266:1302	High portions of muramic acid (MurAc) pointed to the participation of bacteria in the transformation processes.
25909987	9	69	theme	constructors	1931:1942	arg1	niches					1917:1922	the trophic niches	1905:1922	the trophic niches of the constructors	1905:1942	We therewith document that nest chemistry of rainforest termites shows variations and evidence of modification by microbial processes, but nevertheless it primarily reflects the trophic niches of the constructors.
25909987	1	70	theme	feeding	203:209	arg1	habits					211:216	their feeding habits	197:216	their feeding habits	197:216	The impact of termites on nutrient cycling and tropical soil formation depends on their feeding habits and related material transformation.
25909987	3	71	from	genera	495:500	arg1	rainforests					540:550	the Terra Firme rainforests	524:550	the Terra Firme rainforests	524:550	Here, we related the sources and alteration of organic matter in nests from seven different termite genera and feeding habits in the Terra Firme rainforests to the properties of potential food sources soil, wood, and microepiphytes.
25909987	3	71	from	genera	495:500	arg1	nests					460:464	nests	460:464	nests from seven different termite genera and feeding habits in the Terra Firme rainforests	460:550	Here, we related the sources and alteration of organic matter in nests from seven different termite genera and feeding habits in the Terra Firme rainforests to the properties of potential food sources soil, wood, and microepiphytes.
25909987	3	71	from	genera	495:500	arg1	alteration					428:437	alteration	428:437	alteration of organic matter in nests from seven different termite genera and feeding habits in the Terra Firme rainforests	428:550	Here, we related the sources and alteration of organic matter in nests from seven different termite genera and feeding habits in the Terra Firme rainforests to the properties of potential food sources soil, wood, and microepiphytes.
25909987	3	71	from	genera	495:500	arg1	sources					416:422	the sources	412:422	the sources	412:422	Here, we related the sources and alteration of organic matter in nests from seven different termite genera and feeding habits in the Terra Firme rainforests to the properties of potential food sources soil, wood, and microepiphytes.
25909987	0	72	theme	feeding	73:79	arg1	guilds					81:86	different feeding guilds	63:86	different feeding guilds of the Amazon rainforests	63:112	Origin and alteration of organic matter in termite mounds from different feeding guilds of the Amazon rainforests.
25909987	5	73	theme	δ15N	1029:1032	arg1	samples					1005:1011	wood samples	1000:1011	wood samples (δ13C = -29.1‰, δ15N = 3.4‰)	1000:1040	The isotopic analysis revealed higher soil δ13C (-27.4‰) and δ15N (6.6‰) values in nests of wood feeding Nasutitermes and Cornitermes than in wood samples (δ13C = -29.1‰, δ15N = 3.4‰), reflecting stable-isotope enrichment with organic matter alterations during or after nest construction.
25909987	5	73	theme	δ15N	1029:1032	arg1	3.4‰					1036:1039	δ13C = -29.1‰, δ15N = 3.4‰	1014:1039	3.4‰	1036:1039	The isotopic analysis revealed higher soil δ13C (-27.4‰) and δ15N (6.6‰) values in nests of wood feeding Nasutitermes and Cornitermes than in wood samples (δ13C = -29.1‰, δ15N = 3.4‰), reflecting stable-isotope enrichment with organic matter alterations during or after nest construction.
25909987	4	74	theme	isotopic	656:663	arg1	composition					665:675	isotopic composition	656:675	isotopic composition of C and N	656:686	Chemical analyses comprised isotopic composition of C and N, cellulosic (CPS), non-cellulosic (NCPS), and N-containing saccharides, and molecular composition screening using pyrolysis-field ionization mass spectrometry (Py-FIMS).
25909987	0	75	theme	Amazon	95:100	arg1	rainforests					102:112	the Amazon rainforests	91:112	the Amazon rainforests	91:112	Origin and alteration of organic matter in termite mounds from different feeding guilds of the Amazon rainforests.
25909987	5	76	theme	δ13C	1014:1017	arg1	samples					1005:1011	wood samples	1000:1011	wood samples (δ13C = -29.1‰, δ15N = 3.4‰)	1000:1040	The isotopic analysis revealed higher soil δ13C (-27.4‰) and δ15N (6.6‰) values in nests of wood feeding Nasutitermes and Cornitermes than in wood samples (δ13C = -29.1‰, δ15N = 3.4‰), reflecting stable-isotope enrichment with organic matter alterations during or after nest construction.
25909987	5	76	theme	δ13C	1014:1017	arg1	3.4‰					1036:1039	δ13C = -29.1‰, δ15N = 3.4‰	1014:1039	3.4‰	1036:1039	The isotopic analysis revealed higher soil δ13C (-27.4‰) and δ15N (6.6‰) values in nests of wood feeding Nasutitermes and Cornitermes than in wood samples (δ13C = -29.1‰, δ15N = 3.4‰), reflecting stable-isotope enrichment with organic matter alterations during or after nest construction.
25909987	4	77	theme	non-cellulosic	707:720	arg1	saccharides					747:757	cellulosic (CPS), non-cellulosic (NCPS), and N-containing saccharides	689:757	cellulosic (CPS), non-cellulosic (NCPS), and N-containing saccharides	689:757	Chemical analyses comprised isotopic composition of C and N, cellulosic (CPS), non-cellulosic (NCPS), and N-containing saccharides, and molecular composition screening using pyrolysis-field ionization mass spectrometry (Py-FIMS).
25909987	5	78	theme	-29.1‰	1021:1026	arg1	samples					1005:1011	wood samples	1000:1011	wood samples (δ13C = -29.1‰, δ15N = 3.4‰)	1000:1040	The isotopic analysis revealed higher soil δ13C (-27.4‰) and δ15N (6.6‰) values in nests of wood feeding Nasutitermes and Cornitermes than in wood samples (δ13C = -29.1‰, δ15N = 3.4‰), reflecting stable-isotope enrichment with organic matter alterations during or after nest construction.
25909987	5	78	theme	-29.1‰	1021:1026	arg1	3.4‰					1036:1039	δ13C = -29.1‰, δ15N = 3.4‰	1014:1039	3.4‰	1036:1039	The isotopic analysis revealed higher soil δ13C (-27.4‰) and δ15N (6.6‰) values in nests of wood feeding Nasutitermes and Cornitermes than in wood samples (δ13C = -29.1‰, δ15N = 3.4‰), reflecting stable-isotope enrichment with organic matter alterations during or after nest construction.
25909987	1	79	from	impact	119:124	arg1	cycling					150:156	nutrient cycling	141:156	nutrient cycling	141:156	The impact of termites on nutrient cycling and tropical soil formation depends on their feeding habits and related material transformation.
25909987	1	79	from	impact	119:124	arg1	formation					176:184	tropical soil formation	162:184	tropical soil formation	162:184	The impact of termites on nutrient cycling and tropical soil formation depends on their feeding habits and related material transformation.
25909987	5	80	theme	higher	889:894	arg1	δ13C					901:904	higher soil δ13C (-27.4‰) and δ15N (6.6‰) values	889:936	δ13C	901:904	The isotopic analysis revealed higher soil δ13C (-27.4‰) and δ15N (6.6‰) values in nests of wood feeding Nasutitermes and Cornitermes than in wood samples (δ13C = -29.1‰, δ15N = 3.4‰), reflecting stable-isotope enrichment with organic matter alterations during or after nest construction.
25909987	5	80	theme	higher	889:894	arg1	-27.4‰					907:912	-27.4‰	907:912	-27.4‰	907:912	The isotopic analysis revealed higher soil δ13C (-27.4‰) and δ15N (6.6‰) values in nests of wood feeding Nasutitermes and Cornitermes than in wood samples (δ13C = -29.1‰, δ15N = 3.4‰), reflecting stable-isotope enrichment with organic matter alterations during or after nest construction.
25909987	9	81	theme	nest	1758:1761	arg1	chemistry					1763:1771	nest chemistry	1758:1771	nest chemistry of rainforest termites	1758:1794	We therewith document that nest chemistry of rainforest termites shows variations and evidence of modification by microbial processes, but nevertheless it primarily reflects the trophic niches of the constructors.
25909987	0	82	theme	matter	33:38	arg1	alteration					11:20	alteration	11:20	alteration	11:20	Origin and alteration of organic matter in termite mounds from different feeding guilds of the Amazon rainforests.
25909987	0	82	theme	matter	33:38	arg1	Origin					0:5	Origin	0:5	Origin	0:5	Origin and alteration of organic matter in termite mounds from different feeding guilds of the Amazon rainforests.
25909987	6	83	from	decomposition	1238:1250	arg1	nests					1259:1263	the nests	1255:1263	the nests	1255:1263	This result was confirmed by elevated NCPS:CPS ratios, indicating a preferential cellulose decomposition in the nests.
25909987	4	84	theme	N-containing	734:745	arg1	saccharides					747:757	cellulosic (CPS), non-cellulosic (NCPS), and N-containing saccharides	689:757	cellulosic (CPS), non-cellulosic (NCPS), and N-containing saccharides	689:757	Chemical analyses comprised isotopic composition of C and N, cellulosic (CPS), non-cellulosic (NCPS), and N-containing saccharides, and molecular composition screening using pyrolysis-field ionization mass spectrometry (Py-FIMS).
25909987	3	85	from	sources	416:422	arg1	genera					495:500	seven different termite genera	471:500	seven different termite genera	471:500	Here, we related the sources and alteration of organic matter in nests from seven different termite genera and feeding habits in the Terra Firme rainforests to the properties of potential food sources soil, wood, and microepiphytes.
25909987	3	85	from	sources	416:422	arg1	habits					514:519	feeding habits	506:519	feeding habits	506:519	Here, we related the sources and alteration of organic matter in nests from seven different termite genera and feeding habits in the Terra Firme rainforests to the properties of potential food sources soil, wood, and microepiphytes.
25909987	3	85	from	sources	416:422	arg1	nests					460:464	nests	460:464	nests from seven different termite genera and feeding habits in the Terra Firme rainforests	460:550	Here, we related the sources and alteration of organic matter in nests from seven different termite genera and feeding habits in the Terra Firme rainforests to the properties of potential food sources soil, wood, and microepiphytes.
25909987	2	86	theme	nest	376:379	arg1	construction					381:392	nest construction	376:392	nest construction	376:392	The identification of food sources, however, is difficult, because they are variable and changed by termite activity and nest construction.
25909987	0	87	theme	termite	43:49	arg1	mounds					51:56	termite mounds	43:56	termite mounds from different feeding guilds of the Amazon rainforests	43:112	Origin and alteration of organic matter in termite mounds from different feeding guilds of the Amazon rainforests.
25909987	9	88	theme	termites	1787:1794	arg1	chemistry					1763:1771	nest chemistry	1758:1771	nest chemistry of rainforest termites	1758:1794	We therewith document that nest chemistry of rainforest termites shows variations and evidence of modification by microbial processes, but nevertheless it primarily reflects the trophic niches of the constructors.
25909987	3	89	from	alteration	428:437	arg1	genera					495:500	seven different termite genera	471:500	seven different termite genera	471:500	Here, we related the sources and alteration of organic matter in nests from seven different termite genera and feeding habits in the Terra Firme rainforests to the properties of potential food sources soil, wood, and microepiphytes.
25909987	3	89	from	alteration	428:437	arg1	habits					514:519	feeding habits	506:519	feeding habits	506:519	Here, we related the sources and alteration of organic matter in nests from seven different termite genera and feeding habits in the Terra Firme rainforests to the properties of potential food sources soil, wood, and microepiphytes.
25909987	3	89	from	alteration	428:437	arg1	nests					460:464	nests	460:464	nests from seven different termite genera and feeding habits in the Terra Firme rainforests	460:550	Here, we related the sources and alteration of organic matter in nests from seven different termite genera and feeding habits in the Terra Firme rainforests to the properties of potential food sources soil, wood, and microepiphytes.
25909987	5	90	from	δ13C	901:904	arg1	nests					941:945	nests	941:945	nests of wood feeding	941:961	The isotopic analysis revealed higher soil δ13C (-27.4‰) and δ15N (6.6‰) values in nests of wood feeding Nasutitermes and Cornitermes than in wood samples (δ13C = -29.1‰, δ15N = 3.4‰), reflecting stable-isotope enrichment with organic matter alterations during or after nest construction.
25909987	5	91	theme	stable-isotope	1054:1067	arg1	enrichment					1069:1078	stable-isotope enrichment	1054:1078	stable-isotope enrichment with organic matter alterations during or after nest construction	1054:1144	The isotopic analysis revealed higher soil δ13C (-27.4‰) and δ15N (6.6‰) values in nests of wood feeding Nasutitermes and Cornitermes than in wood samples (δ13C = -29.1‰, δ15N = 3.4‰), reflecting stable-isotope enrichment with organic matter alterations during or after nest construction.
25909987	3	92	theme	matter	450:455	arg1	alteration					428:437	alteration	428:437	alteration of organic matter in nests from seven different termite genera and feeding habits in the Terra Firme rainforests	428:550	Here, we related the sources and alteration of organic matter in nests from seven different termite genera and feeding habits in the Terra Firme rainforests to the properties of potential food sources soil, wood, and microepiphytes.
25909987	3	92	theme	matter	450:455	arg1	sources					416:422	the sources	412:422	the sources	412:422	Here, we related the sources and alteration of organic matter in nests from seven different termite genera and feeding habits in the Terra Firme rainforests to the properties of potential food sources soil, wood, and microepiphytes.
25909987	3	93	theme	termite	487:493	arg1	genera					495:500	seven different termite genera	471:500	seven different termite genera	471:500	Here, we related the sources and alteration of organic matter in nests from seven different termite genera and feeding habits in the Terra Firme rainforests to the properties of potential food sources soil, wood, and microepiphytes.
25909987	8	94	theme	Constrictotermes	1590:1605	arg1	material					1578:1585	the nest material	1569:1585	the nest material of Constrictotermes	1569:1605	Non-metric multidimensional scaling (MDS) revealed increasing geophagy in the sequence Termes < Embiratermes < Anoplotermes and increasing xylophagy for Cornitermes < Nasutitermes., and that the nest material of Constrictotermes was similar to the microepiphytes sample, confirming the report that Constrictotermes belongs to the microepiphyte-feeders.
25909987	8	94	theme	Constrictotermes	1590:1605	arg1	similar					1611:1617	similar	1611:1617	similar	1611:1617	Non-metric multidimensional scaling (MDS) revealed increasing geophagy in the sequence Termes < Embiratermes < Anoplotermes and increasing xylophagy for Cornitermes < Nasutitermes., and that the nest material of Constrictotermes was similar to the microepiphytes sample, confirming the report that Constrictotermes belongs to the microepiphyte-feeders.
25909987	1	95	theme	related	222:228	arg1	transformation					239:252	related material transformation	222:252	related material transformation	222:252	The impact of termites on nutrient cycling and tropical soil formation depends on their feeding habits and related material transformation.
27664921	4	0	theme	more	742:745	arg1	inhibition					765:774	more or less important inhibition	742:774	more or less important inhibition of plasma clots	742:790	In vitro anticoagulant activity tests showed in all fractions more or less important inhibition of plasma clots, however, So3 and So4 were the most active.
27664921	1	1	theme	plant	365:369	arg1	material					371:378	the plant material	361:378	the plant material	361:378	A macromolecular complex has been isolated from the dried flowering parts of medicinal plant Sanguisorba officinalis L. (So) by multi-step extraction procedure, including that with extraction by organic solvents to degrease the plant material, then with hot alkali, followed by neutralization, partitioning with organic solvents and dialysis.
27664921	6	2	theme	thrombin	1016:1023	arg1	activity					1004:1011	the activity	1000:1011	the activity of thrombin	1000:1023	These S. officinalis conjugates were able to inhibit mainly the activity of thrombin when they were mediated by heparin cofactor II, but what was unexpected they were the non-direct inhibitors of factor Xa, mediated by antitrombin, where such mechanism of action is typical for a highly sulphated glycosaminoglycans.
27664921	5	3	theme	unfractionated	913:926	arg1	complex					928:934	the unfractionated complex	909:934	the unfractionated complex	909:934	The anticoagulant activity of So3 was even more significant than that of the unfractionated complex So.
27664921	6	4	theme	action	1196:1201	arg1	mechanism					1183:1191	such mechanism	1178:1191	such mechanism of action	1178:1201	These S. officinalis conjugates were able to inhibit mainly the activity of thrombin when they were mediated by heparin cofactor II, but what was unexpected they were the non-direct inhibitors of factor Xa, mediated by antitrombin, where such mechanism of action is typical for a highly sulphated glycosaminoglycans.
27664921	6	5	theme	such	1178:1181	arg1	mechanism					1183:1191	such mechanism	1178:1191	such mechanism of action	1178:1201	These S. officinalis conjugates were able to inhibit mainly the activity of thrombin when they were mediated by heparin cofactor II, but what was unexpected they were the non-direct inhibitors of factor Xa, mediated by antitrombin, where such mechanism of action is typical for a highly sulphated glycosaminoglycans.
27664921	4	6	theme	activity	703:710	arg1	tests					712:716	In vitro anticoagulant activity tests	680:716	In vitro anticoagulant activity tests	680:716	In vitro anticoagulant activity tests showed in all fractions more or less important inhibition of plasma clots, however, So3 and So4 were the most active.
27664921	1	7	theme	multi-step	265:274	arg1	procedure					287:295	multi-step extraction procedure	265:295	multi-step extraction procedure	265:295	A macromolecular complex has been isolated from the dried flowering parts of medicinal plant Sanguisorba officinalis L. (So) by multi-step extraction procedure, including that with extraction by organic solvents to degrease the plant material, then with hot alkali, followed by neutralization, partitioning with organic solvents and dialysis.
27664921	0	8	theme	heparin	116:122	arg1	cofactor					124:131	heparin cofactor II	116:134	heparin cofactor II	116:134	Polyphenolic-polysaccharide conjugates of Sanguisorba officinalis L. with anticoagulant activity mediated mainly by heparin cofactor II.
27664921	1	9	attach	isolated	171:178	arg1	parts					205:209	the dried flowering parts	185:209	the dried flowering parts	185:209	A macromolecular complex has been isolated from the dried flowering parts of medicinal plant Sanguisorba officinalis L. (So) by multi-step extraction procedure, including that with extraction by organic solvents to degrease the plant material, then with hot alkali, followed by neutralization, partitioning with organic solvents and dialysis.
27664921	1	9	attach	isolated	171:178	arg1	that					308:311	that	308:311	that	308:311	A macromolecular complex has been isolated from the dried flowering parts of medicinal plant Sanguisorba officinalis L. (So) by multi-step extraction procedure, including that with extraction by organic solvents to degrease the plant material, then with hot alkali, followed by neutralization, partitioning with organic solvents and dialysis.
27664921	1	9	attach	isolated	171:178	arg2	complex					154:160	A macromolecular complex	137:160	A macromolecular complex	137:160	A macromolecular complex has been isolated from the dried flowering parts of medicinal plant Sanguisorba officinalis L. (So) by multi-step extraction procedure, including that with extraction by organic solvents to degrease the plant material, then with hot alkali, followed by neutralization, partitioning with organic solvents and dialysis.
27664921	1	10	theme	organic	449:455	arg1	solvents					457:464	organic solvents	449:464	organic solvents	449:464	A macromolecular complex has been isolated from the dried flowering parts of medicinal plant Sanguisorba officinalis L. (So) by multi-step extraction procedure, including that with extraction by organic solvents to degrease the plant material, then with hot alkali, followed by neutralization, partitioning with organic solvents and dialysis.
27664921	1	11	theme	extraction	276:285	arg1	procedure					287:295	multi-step extraction procedure	265:295	multi-step extraction procedure	265:295	A macromolecular complex has been isolated from the dried flowering parts of medicinal plant Sanguisorba officinalis L. (So) by multi-step extraction procedure, including that with extraction by organic solvents to degrease the plant material, then with hot alkali, followed by neutralization, partitioning with organic solvents and dialysis.
27664921	5	12	theme	So3	866:868	arg1	activity					854:861	The anticoagulant activity	836:861	The anticoagulant activity of So3	836:868	The anticoagulant activity of So3 was even more significant than that of the unfractionated complex So.
27664921	5	12	theme	So3	866:868	arg1	significant					884:894	significant	884:894	significant	884:894	The anticoagulant activity of So3 was even more significant than that of the unfractionated complex So.
27664921	1	13	theme	dried	189:193	arg1	parts					205:209	the dried flowering parts	185:209	the dried flowering parts	185:209	A macromolecular complex has been isolated from the dried flowering parts of medicinal plant Sanguisorba officinalis L. (So) by multi-step extraction procedure, including that with extraction by organic solvents to degrease the plant material, then with hot alkali, followed by neutralization, partitioning with organic solvents and dialysis.
27664921	1	13	theme	dried	189:193	arg1	that					308:311	that	308:311	that	308:311	A macromolecular complex has been isolated from the dried flowering parts of medicinal plant Sanguisorba officinalis L. (So) by multi-step extraction procedure, including that with extraction by organic solvents to degrease the plant material, then with hot alkali, followed by neutralization, partitioning with organic solvents and dialysis.
27664921	2	14	theme	size-exclusion	508:521	arg1	chromatography					523:536	size-exclusion chromatography	508:536	size-exclusion chromatography	508:536	The complex was purified by size-exclusion chromatography into five fractions labeled as So1-So5.
27664921	1	15	theme	flowering	195:203	arg1	parts					205:209	the dried flowering parts	185:209	the dried flowering parts	185:209	A macromolecular complex has been isolated from the dried flowering parts of medicinal plant Sanguisorba officinalis L. (So) by multi-step extraction procedure, including that with extraction by organic solvents to degrease the plant material, then with hot alkali, followed by neutralization, partitioning with organic solvents and dialysis.
27664921	1	15	theme	flowering	195:203	arg1	that					308:311	that	308:311	that	308:311	A macromolecular complex has been isolated from the dried flowering parts of medicinal plant Sanguisorba officinalis L. (So) by multi-step extraction procedure, including that with extraction by organic solvents to degrease the plant material, then with hot alkali, followed by neutralization, partitioning with organic solvents and dialysis.
27664921	4	16	theme	plasma	779:784	arg1	clots					786:790	plasma clots	779:790	plasma clots	779:790	In vitro anticoagulant activity tests showed in all fractions more or less important inhibition of plasma clots, however, So3 and So4 were the most active.
27664921	0	17	theme	Polyphenolic-polysaccharide	0:26	arg1	conjugates					28:37	Polyphenolic-polysaccharide conjugates	0:37	Polyphenolic-polysaccharide conjugates of Sanguisorba officinalis L. with anticoagulant activity mediated mainly by heparin cofactor II.	0:135	Polyphenolic-polysaccharide conjugates of Sanguisorba officinalis L. with anticoagulant activity mediated mainly by heparin cofactor II.
27664921	6	18	theme	sulphated	1227:1235	arg1	glycosaminoglycans					1237:1254	a highly sulphated glycosaminoglycans	1218:1254	a highly sulphated glycosaminoglycans	1218:1254	These S. officinalis conjugates were able to inhibit mainly the activity of thrombin when they were mediated by heparin cofactor II, but what was unexpected they were the non-direct inhibitors of factor Xa, mediated by antitrombin, where such mechanism of action is typical for a highly sulphated glycosaminoglycans.
27664921	0	19	theme	Sanguisorba	42:52	arg1	L.					66:67	Sanguisorba officinalis L.	42:67	Sanguisorba officinalis L.	42:67	Polyphenolic-polysaccharide conjugates of Sanguisorba officinalis L. with anticoagulant activity mediated mainly by heparin cofactor II.
27664921	6	20	theme	heparin	1052:1058	arg1	cofactor					1060:1067	heparin cofactor II	1052:1070	heparin cofactor II	1052:1070	These S. officinalis conjugates were able to inhibit mainly the activity of thrombin when they were mediated by heparin cofactor II, but what was unexpected they were the non-direct inhibitors of factor Xa, mediated by antitrombin, where such mechanism of action is typical for a highly sulphated glycosaminoglycans.
27664921	1	21	theme	medicinal	214:222	arg1	Sanguisorba					230:240	medicinal plant Sanguisorba	214:240	medicinal plant Sanguisorba officinalis L. (So) by multi-step extraction procedure	214:295	A macromolecular complex has been isolated from the dried flowering parts of medicinal plant Sanguisorba officinalis L. (So) by multi-step extraction procedure, including that with extraction by organic solvents to degrease the plant material, then with hot alkali, followed by neutralization, partitioning with organic solvents and dialysis.
27664921	5	22	theme	anticoagulant	840:852	arg1	activity					854:861	The anticoagulant activity	836:861	The anticoagulant activity of So3	836:868	The anticoagulant activity of So3 was even more significant than that of the unfractionated complex So.
27664921	5	22	theme	anticoagulant	840:852	arg1	significant					884:894	significant	884:894	significant	884:894	The anticoagulant activity of So3 was even more significant than that of the unfractionated complex So.
27664921	3	23	theme	Individual	578:587	arg1	fractions					589:597	Individual fractions	578:597	Individual fractions	578:597	Individual fractions differed in the chemical composition and molecular weight distribution patterns.
27664921	1	24	theme	plant	224:228	arg1	Sanguisorba					230:240	medicinal plant Sanguisorba	214:240	medicinal plant Sanguisorba officinalis L. (So) by multi-step extraction procedure	214:295	A macromolecular complex has been isolated from the dried flowering parts of medicinal plant Sanguisorba officinalis L. (So) by multi-step extraction procedure, including that with extraction by organic solvents to degrease the plant material, then with hot alkali, followed by neutralization, partitioning with organic solvents and dialysis.
27664921	0	25	theme	L.	66:67	arg1	conjugates					28:37	Polyphenolic-polysaccharide conjugates	0:37	Polyphenolic-polysaccharide conjugates of Sanguisorba officinalis L. with anticoagulant activity mediated mainly by heparin cofactor II.	0:135	Polyphenolic-polysaccharide conjugates of Sanguisorba officinalis L. with anticoagulant activity mediated mainly by heparin cofactor II.
27664921	1	26	theme	hot	391:393	arg1	alkali					395:400	hot alkali	391:400	hot alkali	391:400	A macromolecular complex has been isolated from the dried flowering parts of medicinal plant Sanguisorba officinalis L. (So) by multi-step extraction procedure, including that with extraction by organic solvents to degrease the plant material, then with hot alkali, followed by neutralization, partitioning with organic solvents and dialysis.
27664921	3	27	theme	chemical	615:622	arg1	composition					624:634	the chemical composition	611:634	the chemical composition	611:634	Individual fractions differed in the chemical composition and molecular weight distribution patterns.
27664921	1	28	theme	Sanguisorba	230:240	arg1	parts					205:209	the dried flowering parts	185:209	the dried flowering parts	185:209	A macromolecular complex has been isolated from the dried flowering parts of medicinal plant Sanguisorba officinalis L. (So) by multi-step extraction procedure, including that with extraction by organic solvents to degrease the plant material, then with hot alkali, followed by neutralization, partitioning with organic solvents and dialysis.
27664921	1	28	theme	Sanguisorba	230:240	arg1	that					308:311	that	308:311	that	308:311	A macromolecular complex has been isolated from the dried flowering parts of medicinal plant Sanguisorba officinalis L. (So) by multi-step extraction procedure, including that with extraction by organic solvents to degrease the plant material, then with hot alkali, followed by neutralization, partitioning with organic solvents and dialysis.
27664921	0	29	theme	officinalis	54:64	arg1	L.					66:67	Sanguisorba officinalis L.	42:67	Sanguisorba officinalis L.	42:67	Polyphenolic-polysaccharide conjugates of Sanguisorba officinalis L. with anticoagulant activity mediated mainly by heparin cofactor II.
27664921	4	30	dep	In	680:681	arg1	vitro					683:687	vitro	683:687	vitro	683:687	In vitro anticoagulant activity tests showed in all fractions more or less important inhibition of plasma clots, however, So3 and So4 were the most active.
27664921	1	31	theme	officinalis	242:252	arg1	L					254:254	officinalis L	242:254	medicinal plant Sanguisorba officinalis L. (So) by multi-step extraction procedure	214:295	A macromolecular complex has been isolated from the dried flowering parts of medicinal plant Sanguisorba officinalis L. (So) by multi-step extraction procedure, including that with extraction by organic solvents to degrease the plant material, then with hot alkali, followed by neutralization, partitioning with organic solvents and dialysis.
27664921	0	32	theme	anticoagulant	74:86	arg1	activity					88:95	anticoagulant activity	74:95	anticoagulant activity mediated mainly by heparin cofactor II	74:134	Polyphenolic-polysaccharide conjugates of Sanguisorba officinalis L. with anticoagulant activity mediated mainly by heparin cofactor II.
27664921	6	33	dep	conjugates	961:970	arg1	S.					946:947	These S. officinalis conjugates	940:970	These S. officinalis conjugates	940:970	These S. officinalis conjugates were able to inhibit mainly the activity of thrombin when they were mediated by heparin cofactor II, but what was unexpected they were the non-direct inhibitors of factor Xa, mediated by antitrombin, where such mechanism of action is typical for a highly sulphated glycosaminoglycans.
27664921	6	34	theme	factor	1136:1141	arg1	Xa					1143:1144	factor Xa	1136:1144	factor Xa	1136:1144	These S. officinalis conjugates were able to inhibit mainly the activity of thrombin when they were mediated by heparin cofactor II, but what was unexpected they were the non-direct inhibitors of factor Xa, mediated by antitrombin, where such mechanism of action is typical for a highly sulphated glycosaminoglycans.
27664921	6	35	theme	officinalis	949:959	arg1	conjugates					961:970	officinalis conjugates	949:970	These S. officinalis conjugates	940:970	These S. officinalis conjugates were able to inhibit mainly the activity of thrombin when they were mediated by heparin cofactor II, but what was unexpected they were the non-direct inhibitors of factor Xa, mediated by antitrombin, where such mechanism of action is typical for a highly sulphated glycosaminoglycans.
27664921	0	36	with	conjugates	28:37	arg1	activity					88:95	anticoagulant activity	74:95	anticoagulant activity mediated mainly by heparin cofactor II	74:134	Polyphenolic-polysaccharide conjugates of Sanguisorba officinalis L. with anticoagulant activity mediated mainly by heparin cofactor II.
27664921	1	37	theme	macromolecular	139:152	arg1	complex					154:160	A macromolecular complex	137:160	A macromolecular complex	137:160	A macromolecular complex has been isolated from the dried flowering parts of medicinal plant Sanguisorba officinalis L. (So) by multi-step extraction procedure, including that with extraction by organic solvents to degrease the plant material, then with hot alkali, followed by neutralization, partitioning with organic solvents and dialysis.
27664921	1	38	theme	organic	332:338	arg1	solvents					340:347	organic solvents	332:347	organic solvents	332:347	A macromolecular complex has been isolated from the dried flowering parts of medicinal plant Sanguisorba officinalis L. (So) by multi-step extraction procedure, including that with extraction by organic solvents to degrease the plant material, then with hot alkali, followed by neutralization, partitioning with organic solvents and dialysis.
27664921	3	39	theme	weight	650:655	arg1	patterns					670:677	molecular weight distribution patterns	640:677	molecular weight distribution patterns	640:677	Individual fractions differed in the chemical composition and molecular weight distribution patterns.
27664921	1	40	dep	Sanguisorba	230:240	arg1	L					254:254	officinalis L	242:254	medicinal plant Sanguisorba officinalis L. (So) by multi-step extraction procedure	214:295	A macromolecular complex has been isolated from the dried flowering parts of medicinal plant Sanguisorba officinalis L. (So) by multi-step extraction procedure, including that with extraction by organic solvents to degrease the plant material, then with hot alkali, followed by neutralization, partitioning with organic solvents and dialysis.
27664921	1	40	dep	Sanguisorba	230:240	arg1	So					258:259	So	258:259	So	258:259	A macromolecular complex has been isolated from the dried flowering parts of medicinal plant Sanguisorba officinalis L. (So) by multi-step extraction procedure, including that with extraction by organic solvents to degrease the plant material, then with hot alkali, followed by neutralization, partitioning with organic solvents and dialysis.
27664921	6	41	theme	non-direct	1111:1120	arg1	inhibitors					1122:1131	the non-direct inhibitors	1107:1131	the non-direct inhibitors	1107:1131	These S. officinalis conjugates were able to inhibit mainly the activity of thrombin when they were mediated by heparin cofactor II, but what was unexpected they were the non-direct inhibitors of factor Xa, mediated by antitrombin, where such mechanism of action is typical for a highly sulphated glycosaminoglycans.
27664921	6	41	theme	non-direct	1111:1120	arg1	they					1097:1100	they	1097:1100	they	1097:1100	These S. officinalis conjugates were able to inhibit mainly the activity of thrombin when they were mediated by heparin cofactor II, but what was unexpected they were the non-direct inhibitors of factor Xa, mediated by antitrombin, where such mechanism of action is typical for a highly sulphated glycosaminoglycans.
27664921	3	42	theme	distribution	657:668	arg1	patterns					670:677	molecular weight distribution patterns	640:677	molecular weight distribution patterns	640:677	Individual fractions differed in the chemical composition and molecular weight distribution patterns.
27664921	4	43	theme	clots	786:790	arg1	inhibition					765:774	more or less important inhibition	742:774	more or less important inhibition of plasma clots	742:790	In vitro anticoagulant activity tests showed in all fractions more or less important inhibition of plasma clots, however, So3 and So4 were the most active.
27664921	4	44	theme	important	755:763	arg1	inhibition					765:774	more or less important inhibition	742:774	more or less important inhibition of plasma clots	742:790	In vitro anticoagulant activity tests showed in all fractions more or less important inhibition of plasma clots, however, So3 and So4 were the most active.
27664921	4	45	theme	In	680:681	arg1	tests					712:716	In vitro anticoagulant activity tests	680:716	In vitro anticoagulant activity tests	680:716	In vitro anticoagulant activity tests showed in all fractions more or less important inhibition of plasma clots, however, So3 and So4 were the most active.
27664921	4	46	theme	anticoagulant	689:701	arg1	tests					712:716	In vitro anticoagulant activity tests	680:716	In vitro anticoagulant activity tests	680:716	In vitro anticoagulant activity tests showed in all fractions more or less important inhibition of plasma clots, however, So3 and So4 were the most active.
27664921	6	47	theme	Xa	1143:1144	arg1	inhibitors					1122:1131	the non-direct inhibitors	1107:1131	the non-direct inhibitors	1107:1131	These S. officinalis conjugates were able to inhibit mainly the activity of thrombin when they were mediated by heparin cofactor II, but what was unexpected they were the non-direct inhibitors of factor Xa, mediated by antitrombin, where such mechanism of action is typical for a highly sulphated glycosaminoglycans.
27664921	6	47	theme	Xa	1143:1144	arg1	they					1097:1100	they	1097:1100	they	1097:1100	These S. officinalis conjugates were able to inhibit mainly the activity of thrombin when they were mediated by heparin cofactor II, but what was unexpected they were the non-direct inhibitors of factor Xa, mediated by antitrombin, where such mechanism of action is typical for a highly sulphated glycosaminoglycans.
27664921	3	48	theme	molecular	640:648	arg1	patterns					670:677	molecular weight distribution patterns	640:677	molecular weight distribution patterns	640:677	Individual fractions differed in the chemical composition and molecular weight distribution patterns.
24808176	3	0	theme	polymerization	578:591	arg1	degree					568:573	the degree	564:573	the degree of polymerization	564:591	One promising approach to determining monomer composition and controlling the degree of polymerization involves the use of polysaccharide lyases, which catalyze the depolymerization of anionic polysaccharides via a β-elimination mechanism.
24808176	3	1	theme	lyases	628:633	arg1	use					606:608	the use	602:608	the use	602:608	One promising approach to determining monomer composition and controlling the degree of polymerization involves the use of polysaccharide lyases, which catalyze the depolymerization of anionic polysaccharides via a β-elimination mechanism.
24808176	6	2	theme	substrate-binding	1262:1278	arg1	residues					1280:1287	nine putative substrate-binding residues	1248:1287	nine putative substrate-binding residues	1248:1287	Here, we utilize a sequence structure guided approach based on a homology model of Smlt1473 to identify nine putative substrate-binding residues and examine their effect on substrate specificity via site-directed mutagenesis.
24808176	2	3	theme	polymer	335:341	arg1	length					343:348	polymer length	335:348	polymer length	335:348	Chemical composition and polymer length strongly influence the physical and biological properties of the polysaccharide and thus its potential industrial and medical applications.
24808176	9	4	with	comparison	1691:1700	arg1	structure					1727:1735	the high resolution structure	1707:1735	the high resolution structure of Sphingomonas sp	1707:1754	The effect of these mutations was analyzed by comparison with the high resolution structure of Sphingomonas sp.
24808176	3	5	theme	polysaccharides	683:697	arg1	depolymerization					655:670	the depolymerization	651:670	the depolymerization of anionic polysaccharides	651:697	One promising approach to determining monomer composition and controlling the degree of polymerization involves the use of polysaccharide lyases, which catalyze the depolymerization of anionic polysaccharides via a β-elimination mechanism.
24808176	1	6	theme	potential	234:242	arg1	applications					259:270	their potential pharmaceutical applications	228:270	their potential pharmaceutical applications in drug delivery and wound treatment	228:307	Anionic polysaccharides are of growing interest in the biotechnology industry due to their potential pharmaceutical applications in drug delivery and wound treatment.
24808176	6	7	theme	site-directed	1343:1355	arg1	mutagenesis					1357:1367	site-directed mutagenesis	1343:1367	site-directed mutagenesis	1343:1367	Here, we utilize a sequence structure guided approach based on a homology model of Smlt1473 to identify nine putative substrate-binding residues and examine their effect on substrate specificity via site-directed mutagenesis.
24808176	6	8	theme	homology	1209:1216	arg1	model					1218:1222	a homology model	1207:1222	a homology model of Smlt1473 to identify nine putative substrate-binding residues and examine their effect on substrate specificity via site-directed mutagenesis	1207:1367	Here, we utilize a sequence structure guided approach based on a homology model of Smlt1473 to identify nine putative substrate-binding residues and examine their effect on substrate specificity via site-directed mutagenesis.
24808176	4	9	theme	high	821:824	arg1	degree					826:831	a high degree	819:831	a high degree of control	819:842	Utilization of these enzymes for the production of custom-made oligosaccharides requires a high degree of control over substrate specificity.
24808176	11	10	theme	minor	1957:1961	arg1	changes					1963:1969	even minor changes	1952:1969	even minor changes in active site architecture	1952:1997	Overall, our results demonstrate that even minor changes in active site architecture have a significant effect on the substrate specificity of Smlt1473, whose structural plasticity could be applied to the design of highly active and specific polysaccharide lyases.
24808176	1	11	from	applications	259:270	arg1	delivery					280:287	drug delivery	275:287	drug delivery	275:287	Anionic polysaccharides are of growing interest in the biotechnology industry due to their potential pharmaceutical applications in drug delivery and wound treatment.
24808176	1	11	from	applications	259:270	arg1	treatment					299:307	wound treatment	293:307	wound treatment	293:307	Anionic polysaccharides are of growing interest in the biotechnology industry due to their potential pharmaceutical applications in drug delivery and wound treatment.
24808176	11	12	theme	specific	2147:2154	arg1	lyases					2171:2176	highly active and specific polysaccharide lyases	2129:2176	highly active and specific polysaccharide lyases	2129:2176	Overall, our results demonstrate that even minor changes in active site architecture have a significant effect on the substrate specificity of Smlt1473, whose structural plasticity could be applied to the design of highly active and specific polysaccharide lyases.
24808176	1	13	theme	Anionic	143:149	arg1	polysaccharides					151:165	Anionic polysaccharides	143:165	Anionic polysaccharides	143:165	Anionic polysaccharides are of growing interest in the biotechnology industry due to their potential pharmaceutical applications in drug delivery and wound treatment.
24808176	3	14	theme	monomer	528:534	arg1	composition					536:546	monomer composition	528:546	monomer composition	528:546	One promising approach to determining monomer composition and controlling the degree of polymerization involves the use of polysaccharide lyases, which catalyze the depolymerization of anionic polysaccharides via a β-elimination mechanism.
24808176	4	15	theme	custom-made	781:791	arg1	oligosaccharides					793:808	custom-made oligosaccharides	781:808	custom-made oligosaccharides	781:808	Utilization of these enzymes for the production of custom-made oligosaccharides requires a high degree of control over substrate specificity.
24808176	6	16	from	effect	1307:1312	arg1	specificity					1327:1337	substrate specificity	1317:1337	substrate specificity	1317:1337	Here, we utilize a sequence structure guided approach based on a homology model of Smlt1473 to identify nine putative substrate-binding residues and examine their effect on substrate specificity via site-directed mutagenesis.
24808176	9	17	theme	mutations	1665:1673	arg1	effect					1649:1654	The effect	1645:1654	The effect of these mutations	1645:1673	The effect of these mutations was analyzed by comparison with the high resolution structure of Sphingomonas sp.
24808176	11	18	from	effect	2018:2023	arg1	specificity					2042:2052	the substrate specificity	2028:2052	the substrate specificity of Smlt1473	2028:2064	Overall, our results demonstrate that even minor changes in active site architecture have a significant effect on the substrate specificity of Smlt1473, whose structural plasticity could be applied to the design of highly active and specific polysaccharide lyases.
24808176	11	19	theme	polysaccharide	2156:2169	arg1	lyases					2171:2176	highly active and specific polysaccharide lyases	2129:2176	highly active and specific polysaccharide lyases	2129:2176	Overall, our results demonstrate that even minor changes in active site architecture have a significant effect on the substrate specificity of Smlt1473, whose structural plasticity could be applied to the design of highly active and specific polysaccharide lyases.
24808176	5	20	theme	poly-β-d-mannuronic	1081:1099	arg1	poly-ManA					1107:1115	poly-ManA	1107:1115	poly-ManA	1107:1115	Previously, we characterized a polysaccharide lyase (Smlt1473) from Stenotrophomonas maltophilia k279a, which exhibited significant activity against hyaluronan (HA), poly-β-d-glucuronic acid (poly-GlcUA), and poly-β-d-mannuronic acid (poly-ManA) in a pH-regulated manner.
24808176	5	20	theme	poly-β-d-mannuronic	1081:1099	arg1	acid					1101:1104	poly-β-d-mannuronic acid	1081:1104	poly-β-d-mannuronic acid (poly-ManA)	1081:1116	Previously, we characterized a polysaccharide lyase (Smlt1473) from Stenotrophomonas maltophilia k279a, which exhibited significant activity against hyaluronan (HA), poly-β-d-glucuronic acid (poly-GlcUA), and poly-β-d-mannuronic acid (poly-ManA) in a pH-regulated manner.
24808176	10	21	theme	poly-ManA	1795:1803	arg1	tetrasaccharide					1805:1819	poly-ManA tetrasaccharide	1795:1819	poly-ManA tetrasaccharide	1795:1819	A1-III alginate lyase in complex with poly-ManA tetrasaccharide and by taking into account the structural differences between HA, poly-GlcUA, and poly-ManA.
24808176	0	22	theme	polysaccharide	112:125	arg1	Smlt1473					133:140	the multifunctional polysaccharide lyase Smlt1473	92:140	the multifunctional polysaccharide lyase Smlt1473	92:140	Insight into the role of substrate-binding residues in conferring substrate specificity for the multifunctional polysaccharide lyase Smlt1473.
24808176	6	23	theme	guided	1182:1187	arg1	approach					1189:1196	a sequence structure guided approach	1161:1196	a sequence structure guided approach based on a homology model of Smlt1473 to identify nine putative substrate-binding residues and examine their effect on substrate specificity via site-directed mutagenesis	1161:1367	Here, we utilize a sequence structure guided approach based on a homology model of Smlt1473 to identify nine putative substrate-binding residues and examine their effect on substrate specificity via site-directed mutagenesis.
24808176	11	24	theme	substrate	2032:2040	arg1	specificity					2042:2052	the substrate specificity	2028:2052	the substrate specificity of Smlt1473	2028:2064	Overall, our results demonstrate that even minor changes in active site architecture have a significant effect on the substrate specificity of Smlt1473, whose structural plasticity could be applied to the design of highly active and specific polysaccharide lyases.
24808176	6	25	theme	sequence	1163:1170	arg1	approach					1189:1196	a sequence structure guided approach	1161:1196	a sequence structure guided approach based on a homology model of Smlt1473 to identify nine putative substrate-binding residues and examine their effect on substrate specificity via site-directed mutagenesis	1161:1367	Here, we utilize a sequence structure guided approach based on a homology model of Smlt1473 to identify nine putative substrate-binding residues and examine their effect on substrate specificity via site-directed mutagenesis.
24808176	11	26	theme	structural	2073:2082	arg1	plasticity					2084:2093	plasticity	2084:2093	plasticity	2084:2093	Overall, our results demonstrate that even minor changes in active site architecture have a significant effect on the substrate specificity of Smlt1473, whose structural plasticity could be applied to the design of highly active and specific polysaccharide lyases.
24808176	11	27	theme	active	2136:2141	arg1	lyases					2171:2176	highly active and specific polysaccharide lyases	2129:2176	highly active and specific polysaccharide lyases	2129:2176	Overall, our results demonstrate that even minor changes in active site architecture have a significant effect on the substrate specificity of Smlt1473, whose structural plasticity could be applied to the design of highly active and specific polysaccharide lyases.
24808176	9	28	theme	resolution	1716:1725	arg1	structure					1727:1735	the high resolution structure	1707:1735	the high resolution structure of Sphingomonas sp	1707:1754	The effect of these mutations was analyzed by comparison with the high resolution structure of Sphingomonas sp.
24808176	3	29	theme	promising	494:502	arg1	approach					504:511	One promising approach	490:511	One promising approach to determining monomer composition and controlling the degree of polymerization	490:591	One promising approach to determining monomer composition and controlling the degree of polymerization involves the use of polysaccharide lyases, which catalyze the depolymerization of anionic polysaccharides via a β-elimination mechanism.
24808176	10	30	theme	structural	1852:1861	arg1	differences					1863:1873	the structural differences	1848:1873	the structural differences between HA, poly-GlcUA, and poly-ManA	1848:1911	A1-III alginate lyase in complex with poly-ManA tetrasaccharide and by taking into account the structural differences between HA, poly-GlcUA, and poly-ManA.
24808176	0	31	theme	residues	43:50	arg1	role					17:20	the role	13:20	the role of substrate-binding residues in conferring substrate specificity for the multifunctional polysaccharide lyase Smlt1473	13:140	Insight into the role of substrate-binding residues in conferring substrate specificity for the multifunctional polysaccharide lyase Smlt1473.
24808176	9	32	theme	sp	1753:1754	arg1	structure					1727:1735	the high resolution structure	1707:1735	the high resolution structure of Sphingomonas sp	1707:1754	The effect of these mutations was analyzed by comparison with the high resolution structure of Sphingomonas sp.
24808176	6	33	theme	Smlt1473	1227:1234	arg1	model					1218:1222	a homology model	1207:1222	a homology model of Smlt1473 to identify nine putative substrate-binding residues and examine their effect on substrate specificity via site-directed mutagenesis	1207:1367	Here, we utilize a sequence structure guided approach based on a homology model of Smlt1473 to identify nine putative substrate-binding residues and examine their effect on substrate specificity via site-directed mutagenesis.
24808176	11	34	theme	site	1981:1984	arg1	architecture					1986:1997	active site architecture	1974:1997	active site architecture	1974:1997	Overall, our results demonstrate that even minor changes in active site architecture have a significant effect on the substrate specificity of Smlt1473, whose structural plasticity could be applied to the design of highly active and specific polysaccharide lyases.
24808176	11	35	contain	have	1999:2002	arg1	changes					1963:1969	even minor changes	1952:1969	even minor changes in active site architecture	1952:1997	Overall, our results demonstrate that even minor changes in active site architecture have a significant effect on the substrate specificity of Smlt1473, whose structural plasticity could be applied to the design of highly active and specific polysaccharide lyases.
24808176	11	35	contain	have	1999:2002	arg2	effect					2018:2023	a significant effect	2004:2023	a significant effect	2004:2023	Overall, our results demonstrate that even minor changes in active site architecture have a significant effect on the substrate specificity of Smlt1473, whose structural plasticity could be applied to the design of highly active and specific polysaccharide lyases.
24808176	2	36	theme	polysaccharide	415:428	arg1	properties					397:406	the physical and biological properties	369:406	the physical and biological properties of the polysaccharide	369:428	Chemical composition and polymer length strongly influence the physical and biological properties of the polysaccharide and thus its potential industrial and medical applications.
24808176	10	37	with	lyase	1773:1777	arg1	tetrasaccharide					1805:1819	poly-ManA tetrasaccharide	1795:1819	poly-ManA tetrasaccharide	1795:1819	A1-III alginate lyase in complex with poly-ManA tetrasaccharide and by taking into account the structural differences between HA, poly-GlcUA, and poly-ManA.
24808176	10	37	with	lyase	1773:1777	arg1	taking					1828:1833	taking	1828:1833	taking into account the structural differences between HA, poly-GlcUA, and poly-ManA	1828:1911	A1-III alginate lyase in complex with poly-ManA tetrasaccharide and by taking into account the structural differences between HA, poly-GlcUA, and poly-ManA.
24808176	7	38	theme	point	1392:1396	arg1	H221F					1408:1412	H221F	1408:1412	H221F	1408:1412	Interestingly, single point mutations H221F and R312L resulted in increased activity and specificity toward poly-ManA and poly-GlcUA, respectively.
24808176	7	38	theme	point	1392:1396	arg1	mutations					1398:1406	single point mutations	1385:1406	single point mutations H221F and R312L	1385:1422	Interestingly, single point mutations H221F and R312L resulted in increased activity and specificity toward poly-ManA and poly-GlcUA, respectively.
24808176	7	38	theme	point	1392:1396	arg1	R312L					1418:1422	R312L	1418:1422	R312L	1418:1422	Interestingly, single point mutations H221F and R312L resulted in increased activity and specificity toward poly-ManA and poly-GlcUA, respectively.
24808176	11	39	theme	significant	2006:2016	arg1	effect					2018:2023	a significant effect	2004:2023	a significant effect	2004:2023	Overall, our results demonstrate that even minor changes in active site architecture have a significant effect on the substrate specificity of Smlt1473, whose structural plasticity could be applied to the design of highly active and specific polysaccharide lyases.
24808176	11	40	from	changes	1963:1969	arg1	architecture					1986:1997	active site architecture	1974:1997	active site architecture	1974:1997	Overall, our results demonstrate that even minor changes in active site architecture have a significant effect on the substrate specificity of Smlt1473, whose structural plasticity could be applied to the design of highly active and specific polysaccharide lyases.
24808176	3	41	theme	polysaccharide	613:626	arg1	lyases					628:633	polysaccharide lyases	613:633	polysaccharide lyases	613:633	One promising approach to determining monomer composition and controlling the degree of polymerization involves the use of polysaccharide lyases, which catalyze the depolymerization of anionic polysaccharides via a β-elimination mechanism.
24808176	1	42	theme	wound	293:297	arg1	treatment					299:307	wound treatment	293:307	wound treatment	293:307	Anionic polysaccharides are of growing interest in the biotechnology industry due to their potential pharmaceutical applications in drug delivery and wound treatment.
24808176	2	43	theme	biological	386:395	arg1	properties					397:406	the physical and biological properties	369:406	the physical and biological properties of the polysaccharide	369:428	Chemical composition and polymer length strongly influence the physical and biological properties of the polysaccharide and thus its potential industrial and medical applications.
24808176	2	44	theme	Chemical	310:317	arg1	composition					319:329	Chemical composition	310:329	Chemical composition	310:329	Chemical composition and polymer length strongly influence the physical and biological properties of the polysaccharide and thus its potential industrial and medical applications.
24808176	1	45	theme	biotechnology	198:210	arg1	industry					212:219	the biotechnology industry	194:219	the biotechnology industry due to their potential pharmaceutical applications in drug delivery and wound treatment	194:307	Anionic polysaccharides are of growing interest in the biotechnology industry due to their potential pharmaceutical applications in drug delivery and wound treatment.
24808176	10	46	from	lyase	1773:1777	arg1	complex					1782:1788	complex	1782:1788	complex	1782:1788	A1-III alginate lyase in complex with poly-ManA tetrasaccharide and by taking into account the structural differences between HA, poly-GlcUA, and poly-ManA.
24808176	4	47	theme	enzymes	751:757	arg1	Utilization					730:740	Utilization	730:740	Utilization of these enzymes for the production of custom-made oligosaccharides	730:808	Utilization of these enzymes for the production of custom-made oligosaccharides requires a high degree of control over substrate specificity.
24808176	5	48	theme	significant	992:1002	arg1	activity					1004:1011	significant activity	992:1011	significant activity against hyaluronan (HA), poly-β-d-glucuronic acid (poly-GlcUA), and poly-β-d-mannuronic acid (poly-ManA)	992:1116	Previously, we characterized a polysaccharide lyase (Smlt1473) from Stenotrophomonas maltophilia k279a, which exhibited significant activity against hyaluronan (HA), poly-β-d-glucuronic acid (poly-GlcUA), and poly-β-d-mannuronic acid (poly-ManA) in a pH-regulated manner.
24808176	6	49	theme	putative	1253:1260	arg1	residues					1280:1287	nine putative substrate-binding residues	1248:1287	nine putative substrate-binding residues	1248:1287	Here, we utilize a sequence structure guided approach based on a homology model of Smlt1473 to identify nine putative substrate-binding residues and examine their effect on substrate specificity via site-directed mutagenesis.
24808176	3	50	dep	approach	504:511	arg1	determining					516:526	determining	516:526	determining monomer composition	516:546	One promising approach to determining monomer composition and controlling the degree of polymerization involves the use of polysaccharide lyases, which catalyze the depolymerization of anionic polysaccharides via a β-elimination mechanism.
24808176	3	50	dep	approach	504:511	arg1	controlling					552:562	controlling	552:562	controlling the degree of polymerization	552:591	One promising approach to determining monomer composition and controlling the degree of polymerization involves the use of polysaccharide lyases, which catalyze the depolymerization of anionic polysaccharides via a β-elimination mechanism.
24808176	11	51	theme	lyases	2171:2176	arg1	design					2119:2124	the design	2115:2124	the design of highly active and specific polysaccharide lyases	2115:2176	Overall, our results demonstrate that even minor changes in active site architecture have a significant effect on the substrate specificity of Smlt1473, whose structural plasticity could be applied to the design of highly active and specific polysaccharide lyases.
24808176	1	52	theme	due	221:223	arg1	industry					212:219	the biotechnology industry	194:219	the biotechnology industry due to their potential pharmaceutical applications in drug delivery and wound treatment	194:307	Anionic polysaccharides are of growing interest in the biotechnology industry due to their potential pharmaceutical applications in drug delivery and wound treatment.
24808176	3	53	theme	anionic	675:681	arg1	polysaccharides					683:697	anionic polysaccharides	675:697	anionic polysaccharides	675:697	One promising approach to determining monomer composition and controlling the degree of polymerization involves the use of polysaccharide lyases, which catalyze the depolymerization of anionic polysaccharides via a β-elimination mechanism.
24808176	8	54	theme	poly-GlcUA	1608:1617	arg1	activity					1619:1626	poly-GlcUA activity	1608:1626	poly-GlcUA activity	1608:1626	Furthermore, a W171A mutant nearly eliminated HA activity, while increasing poly-ManA and poly-GlcUA activity by at least 35%.
24808176	10	55	theme	A1-III	1757:1762	arg1	lyase					1773:1777	A1-III alginate lyase	1757:1777	A1-III alginate lyase in complex with poly-ManA tetrasaccharide and by taking into account the structural differences between HA, poly-GlcUA, and poly-ManA.	1757:1912	A1-III alginate lyase in complex with poly-ManA tetrasaccharide and by taking into account the structural differences between HA, poly-GlcUA, and poly-ManA.
24808176	11	56	theme	active	1974:1979	arg1	architecture					1986:1997	active site architecture	1974:1997	active site architecture	1974:1997	Overall, our results demonstrate that even minor changes in active site architecture have a significant effect on the substrate specificity of Smlt1473, whose structural plasticity could be applied to the design of highly active and specific polysaccharide lyases.
24808176	3	57	theme	β-elimination	705:717	arg1	mechanism					719:727	a β-elimination mechanism	703:727	a β-elimination mechanism	703:727	One promising approach to determining monomer composition and controlling the degree of polymerization involves the use of polysaccharide lyases, which catalyze the depolymerization of anionic polysaccharides via a β-elimination mechanism.
24808176	5	58	theme	poly-β-d-glucuronic	1038:1056	arg1	poly-GlcUA					1064:1073	poly-GlcUA	1064:1073	poly-GlcUA	1064:1073	Previously, we characterized a polysaccharide lyase (Smlt1473) from Stenotrophomonas maltophilia k279a, which exhibited significant activity against hyaluronan (HA), poly-β-d-glucuronic acid (poly-GlcUA), and poly-β-d-mannuronic acid (poly-ManA) in a pH-regulated manner.
24808176	5	58	theme	poly-β-d-glucuronic	1038:1056	arg1	acid					1058:1061	poly-β-d-glucuronic acid	1038:1061	poly-β-d-glucuronic acid (poly-GlcUA)	1038:1074	Previously, we characterized a polysaccharide lyase (Smlt1473) from Stenotrophomonas maltophilia k279a, which exhibited significant activity against hyaluronan (HA), poly-β-d-glucuronic acid (poly-GlcUA), and poly-β-d-mannuronic acid (poly-ManA) in a pH-regulated manner.
24808176	6	59	theme	substrate	1317:1325	arg1	specificity					1327:1337	substrate specificity	1317:1337	substrate specificity	1317:1337	Here, we utilize a sequence structure guided approach based on a homology model of Smlt1473 to identify nine putative substrate-binding residues and examine their effect on substrate specificity via site-directed mutagenesis.
24808176	2	60	theme	physical	373:380	arg1	properties					397:406	the physical and biological properties	369:406	the physical and biological properties of the polysaccharide	369:428	Chemical composition and polymer length strongly influence the physical and biological properties of the polysaccharide and thus its potential industrial and medical applications.
24808176	0	61	theme	substrate	66:74	arg1	specificity					76:86	substrate specificity	66:86	substrate specificity for the multifunctional polysaccharide lyase Smlt1473	66:140	Insight into the role of substrate-binding residues in conferring substrate specificity for the multifunctional polysaccharide lyase Smlt1473.
24808176	1	62	theme	growing	174:180	arg1	interest					182:189	growing interest	174:189	growing interest	174:189	Anionic polysaccharides are of growing interest in the biotechnology industry due to their potential pharmaceutical applications in drug delivery and wound treatment.
24808176	4	63	theme	oligosaccharides	793:808	arg1	production					767:776	the production	763:776	the production of custom-made oligosaccharides	763:808	Utilization of these enzymes for the production of custom-made oligosaccharides requires a high degree of control over substrate specificity.
24808176	0	64	theme	multifunctional	96:110	arg1	Smlt1473					133:140	the multifunctional polysaccharide lyase Smlt1473	92:140	the multifunctional polysaccharide lyase Smlt1473	92:140	Insight into the role of substrate-binding residues in conferring substrate specificity for the multifunctional polysaccharide lyase Smlt1473.
24808176	1	65	dep	potential	234:242	arg1	pharmaceutical					244:257	pharmaceutical	244:257	pharmaceutical	244:257	Anionic polysaccharides are of growing interest in the biotechnology industry due to their potential pharmaceutical applications in drug delivery and wound treatment.
24808176	1	66	from	interest	182:189	arg1	industry					212:219	the biotechnology industry	194:219	the biotechnology industry due to their potential pharmaceutical applications in drug delivery and wound treatment	194:307	Anionic polysaccharides are of growing interest in the biotechnology industry due to their potential pharmaceutical applications in drug delivery and wound treatment.
24808176	7	67	dep	mutations	1398:1406	arg1	H221F					1408:1412	H221F	1408:1412	H221F	1408:1412	Interestingly, single point mutations H221F and R312L resulted in increased activity and specificity toward poly-ManA and poly-GlcUA, respectively.
24808176	7	67	dep	mutations	1398:1406	arg1	mutations					1398:1406	single point mutations	1385:1406	single point mutations H221F and R312L	1385:1422	Interestingly, single point mutations H221F and R312L resulted in increased activity and specificity toward poly-ManA and poly-GlcUA, respectively.
24808176	7	67	dep	mutations	1398:1406	arg1	R312L					1418:1422	R312L	1418:1422	R312L	1418:1422	Interestingly, single point mutations H221F and R312L resulted in increased activity and specificity toward poly-ManA and poly-GlcUA, respectively.
24808176	0	68	theme	lyase	127:131	arg1	Smlt1473					133:140	the multifunctional polysaccharide lyase Smlt1473	92:140	the multifunctional polysaccharide lyase Smlt1473	92:140	Insight into the role of substrate-binding residues in conferring substrate specificity for the multifunctional polysaccharide lyase Smlt1473.
24808176	11	69	theme	Smlt1473	2057:2064	arg1	specificity					2042:2052	the substrate specificity	2028:2052	the substrate specificity of Smlt1473	2028:2064	Overall, our results demonstrate that even minor changes in active site architecture have a significant effect on the substrate specificity of Smlt1473, whose structural plasticity could be applied to the design of highly active and specific polysaccharide lyases.
24808176	6	70	theme	structure	1172:1180	arg1	approach					1189:1196	a sequence structure guided approach	1161:1196	a sequence structure guided approach based on a homology model of Smlt1473 to identify nine putative substrate-binding residues and examine their effect on substrate specificity via site-directed mutagenesis	1161:1367	Here, we utilize a sequence structure guided approach based on a homology model of Smlt1473 to identify nine putative substrate-binding residues and examine their effect on substrate specificity via site-directed mutagenesis.
24808176	4	71	theme	control	836:842	arg1	degree					826:831	a high degree	819:831	a high degree of control	819:842	Utilization of these enzymes for the production of custom-made oligosaccharides requires a high degree of control over substrate specificity.
24808176	9	72	theme	high	1711:1714	arg1	structure					1727:1735	the high resolution structure	1707:1735	the high resolution structure of Sphingomonas sp	1707:1754	The effect of these mutations was analyzed by comparison with the high resolution structure of Sphingomonas sp.
24808176	7	73	theme	increased	1436:1444	arg1	activity					1446:1453	activity	1446:1453	activity	1446:1453	Interestingly, single point mutations H221F and R312L resulted in increased activity and specificity toward poly-ManA and poly-GlcUA, respectively.
24808176	4	74	theme	substrate	849:857	arg1	specificity					859:869	substrate specificity	849:869	substrate specificity	849:869	Utilization of these enzymes for the production of custom-made oligosaccharides requires a high degree of control over substrate specificity.
24808176	2	75	theme	medical	468:474	arg1	applications					476:487	its potential industrial and medical applications	439:487	its potential industrial and medical applications	439:487	Chemical composition and polymer length strongly influence the physical and biological properties of the polysaccharide and thus its potential industrial and medical applications.
24808176	0	76	theme	substrate-binding	25:41	arg1	residues					43:50	substrate-binding residues	25:50	substrate-binding residues	25:50	Insight into the role of substrate-binding residues in conferring substrate specificity for the multifunctional polysaccharide lyase Smlt1473.
24808176	5	77	theme	pH-regulated	1123:1134	arg1	manner					1136:1141	a pH-regulated manner	1121:1141	a pH-regulated manner	1121:1141	Previously, we characterized a polysaccharide lyase (Smlt1473) from Stenotrophomonas maltophilia k279a, which exhibited significant activity against hyaluronan (HA), poly-β-d-glucuronic acid (poly-GlcUA), and poly-β-d-mannuronic acid (poly-ManA) in a pH-regulated manner.
24808176	2	78	theme	potential	443:451	arg1	applications					476:487	its potential industrial and medical applications	439:487	its potential industrial and medical applications	439:487	Chemical composition and polymer length strongly influence the physical and biological properties of the polysaccharide and thus its potential industrial and medical applications.
24808176	10	79	theme	alginate	1764:1771	arg1	lyase					1773:1777	A1-III alginate lyase	1757:1777	A1-III alginate lyase in complex with poly-ManA tetrasaccharide and by taking into account the structural differences between HA, poly-GlcUA, and poly-ManA.	1757:1912	A1-III alginate lyase in complex with poly-ManA tetrasaccharide and by taking into account the structural differences between HA, poly-GlcUA, and poly-ManA.
24808176	8	80	theme	W171A	1533:1537	arg1	mutant					1539:1544	a W171A mutant	1531:1544	a W171A mutant	1531:1544	Furthermore, a W171A mutant nearly eliminated HA activity, while increasing poly-ManA and poly-GlcUA activity by at least 35%.
24808176	2	81	theme	industrial	453:462	arg1	applications					476:487	its potential industrial and medical applications	439:487	its potential industrial and medical applications	439:487	Chemical composition and polymer length strongly influence the physical and biological properties of the polysaccharide and thus its potential industrial and medical applications.
24808176	5	82	theme	polysaccharide	903:916	arg1	Smlt1473					925:932	Smlt1473	925:932	Smlt1473	925:932	Previously, we characterized a polysaccharide lyase (Smlt1473) from Stenotrophomonas maltophilia k279a, which exhibited significant activity against hyaluronan (HA), poly-β-d-glucuronic acid (poly-GlcUA), and poly-β-d-mannuronic acid (poly-ManA) in a pH-regulated manner.
24808176	5	82	theme	polysaccharide	903:916	arg1	lyase					918:922	a polysaccharide lyase	901:922	a polysaccharide lyase (Smlt1473)	901:933	Previously, we characterized a polysaccharide lyase (Smlt1473) from Stenotrophomonas maltophilia k279a, which exhibited significant activity against hyaluronan (HA), poly-β-d-glucuronic acid (poly-GlcUA), and poly-β-d-mannuronic acid (poly-ManA) in a pH-regulated manner.
24808176	9	83	theme	Sphingomonas	1740:1751	arg1	sp					1753:1754	Sphingomonas sp	1740:1754	Sphingomonas sp	1740:1754	The effect of these mutations was analyzed by comparison with the high resolution structure of Sphingomonas sp.
24808176	8	84	theme	HA	1564:1565	arg1	activity					1567:1574	HA activity	1564:1574	HA activity	1564:1574	Furthermore, a W171A mutant nearly eliminated HA activity, while increasing poly-ManA and poly-GlcUA activity by at least 35%.
24808176	7	85	theme	single	1385:1390	arg1	H221F					1408:1412	H221F	1408:1412	H221F	1408:1412	Interestingly, single point mutations H221F and R312L resulted in increased activity and specificity toward poly-ManA and poly-GlcUA, respectively.
24808176	7	85	theme	single	1385:1390	arg1	mutations					1398:1406	single point mutations	1385:1406	single point mutations H221F and R312L	1385:1422	Interestingly, single point mutations H221F and R312L resulted in increased activity and specificity toward poly-ManA and poly-GlcUA, respectively.
24808176	7	85	theme	single	1385:1390	arg1	R312L					1418:1422	R312L	1418:1422	R312L	1418:1422	Interestingly, single point mutations H221F and R312L resulted in increased activity and specificity toward poly-ManA and poly-GlcUA, respectively.
24808176	1	86	theme	drug	275:278	arg1	delivery					280:287	drug delivery	275:287	drug delivery	275:287	Anionic polysaccharides are of growing interest in the biotechnology industry due to their potential pharmaceutical applications in drug delivery and wound treatment.
24679094	4	0	theme	highest	771:777	arg1	content					785:791	The highest lipid content	767:791	The highest lipid content in the activated sludge	767:815	The highest lipid content in the activated sludge was 95.6 mg/g VSS, obtained at 30°C, 2 mg/L of DO and a SRT of 2 days.
24679094	4	0	theme	highest	771:777	arg1	VSS					831:833	95.6 mg/g VSS	821:833	95.6 mg/g VSS	821:833	The highest lipid content in the activated sludge was 95.6 mg/g VSS, obtained at 30°C, 2 mg/L of DO and a SRT of 2 days.
24679094	1	1	theme	activated	343:351	arg1	system					360:365	a conventional activated sludge system	328:365	a conventional activated sludge system fed with synthetic sewage water at an organic load rate of 1.0 kg COD/(m(3)·d)	328:444	The macromolecular composition of activated sludge (lipids, intracellular proteins and intracellular polysaccharides) was studied together with its capacity to store macromolecules such as polyhydroxybutyrate (PHB) in a conventional activated sludge system fed with synthetic sewage water at an organic load rate of 1.0 kg COD/(m(3)·d), varying the dissolved oxygen (DO) and temperature.
24679094	2	2	theme	retention	592:600	arg1	time					602:605	a sludge retention time	583:605	a sludge retention time (SRT) of 6 days	583:621	Six DO concentrations (0.8, 1.0, 1.5, 2.0, 2.5 and 8 mg/L) were studied at 20°C with a sludge retention time (SRT) of 6 days.
24679094	2	2	theme	retention	592:600	arg1	SRT					608:610	SRT	608:610	SRT	608:610	Six DO concentrations (0.8, 1.0, 1.5, 2.0, 2.5 and 8 mg/L) were studied at 20°C with a sludge retention time (SRT) of 6 days.
24679094	4	3	theme	mg/g	826:829	arg1	VSS					831:833	95.6 mg/g VSS	821:833	95.6 mg/g VSS	821:833	The highest lipid content in the activated sludge was 95.6 mg/g VSS, obtained at 30°C, 2 mg/L of DO and a SRT of 2 days.
24679094	4	3	theme	mg/g	826:829	arg1	content					785:791	The highest lipid content	767:791	The highest lipid content in the activated sludge	767:815	The highest lipid content in the activated sludge was 95.6 mg/g VSS, obtained at 30°C, 2 mg/L of DO and a SRT of 2 days.
24679094	7	4	theme	activated	1197:1205	arg1	storage					1218:1224	The activated sludge PHB storage	1193:1224	The activated sludge PHB storage	1193:1224	The activated sludge PHB storage was very low for all the conditions studied.
24679094	7	4	theme	activated	1197:1205	arg1	low					1235:1237	low	1235:1237	low	1235:1237	The activated sludge PHB storage was very low for all the conditions studied.
24679094	0	5	theme	PHB	77:79	arg1	storage					81:87	PHB storage	77:87	PHB storage	77:87	Effect of dissolved oxygen and temperature on macromolecular composition and PHB storage of activated sludge.
24679094	1	6	theme	sludge	353:358	arg1	system					360:365	a conventional activated sludge system	328:365	a conventional activated sludge system fed with synthetic sewage water at an organic load rate of 1.0 kg COD/(m(3)·d)	328:444	The macromolecular composition of activated sludge (lipids, intracellular proteins and intracellular polysaccharides) was studied together with its capacity to store macromolecules such as polyhydroxybutyrate (PHB) in a conventional activated sludge system fed with synthetic sewage water at an organic load rate of 1.0 kg COD/(m(3)·d), varying the dissolved oxygen (DO) and temperature.
24679094	1	7	theme	kg	430:431	arg1	m					438:438	m(3)·d	438:443	m(3)·d	438:443	The macromolecular composition of activated sludge (lipids, intracellular proteins and intracellular polysaccharides) was studied together with its capacity to store macromolecules such as polyhydroxybutyrate (PHB) in a conventional activated sludge system fed with synthetic sewage water at an organic load rate of 1.0 kg COD/(m(3)·d), varying the dissolved oxygen (DO) and temperature.
24679094	1	7	theme	kg	430:431	arg1	COD/					433:436	1.0 kg COD/	426:436	1.0 kg COD/(m(3)·d)	426:444	The macromolecular composition of activated sludge (lipids, intracellular proteins and intracellular polysaccharides) was studied together with its capacity to store macromolecules such as polyhydroxybutyrate (PHB) in a conventional activated sludge system fed with synthetic sewage water at an organic load rate of 1.0 kg COD/(m(3)·d), varying the dissolved oxygen (DO) and temperature.
24679094	7	8	theme	sludge	1207:1212	arg1	storage					1218:1224	The activated sludge PHB storage	1193:1224	The activated sludge PHB storage	1193:1224	The activated sludge PHB storage was very low for all the conditions studied.
24679094	7	8	theme	sludge	1207:1212	arg1	low					1235:1237	low	1235:1237	low	1235:1237	The activated sludge PHB storage was very low for all the conditions studied.
24679094	0	9	theme	activated	92:100	arg1	sludge					102:107	activated sludge	92:107	activated sludge	92:107	Effect of dissolved oxygen and temperature on macromolecular composition and PHB storage of activated sludge.
24679094	0	10	from	Effect	0:5	arg1	composition					61:71	macromolecular composition	46:71	macromolecular composition	46:71	Effect of dissolved oxygen and temperature on macromolecular composition and PHB storage of activated sludge.
24679094	0	10	from	Effect	0:5	arg1	storage					81:87	PHB storage	77:87	PHB storage	77:87	Effect of dissolved oxygen and temperature on macromolecular composition and PHB storage of activated sludge.
24679094	1	11	theme	COD/	433:436	arg1	rate					418:421	an organic load rate	402:421	an organic load rate of 1.0 kg COD/(m(3)·d)	402:444	The macromolecular composition of activated sludge (lipids, intracellular proteins and intracellular polysaccharides) was studied together with its capacity to store macromolecules such as polyhydroxybutyrate (PHB) in a conventional activated sludge system fed with synthetic sewage water at an organic load rate of 1.0 kg COD/(m(3)·d), varying the dissolved oxygen (DO) and temperature.
24679094	1	12	theme	store	270:274	arg1	polyhydroxybutyrate					299:317	polyhydroxybutyrate	299:317	polyhydroxybutyrate (PHB)	299:323	The macromolecular composition of activated sludge (lipids, intracellular proteins and intracellular polysaccharides) was studied together with its capacity to store macromolecules such as polyhydroxybutyrate (PHB) in a conventional activated sludge system fed with synthetic sewage water at an organic load rate of 1.0 kg COD/(m(3)·d), varying the dissolved oxygen (DO) and temperature.
24679094	1	12	theme	store	270:274	arg1	macromolecules					276:289	store macromolecules	270:289	store macromolecules such as polyhydroxybutyrate (PHB)	270:323	The macromolecular composition of activated sludge (lipids, intracellular proteins and intracellular polysaccharides) was studied together with its capacity to store macromolecules such as polyhydroxybutyrate (PHB) in a conventional activated sludge system fed with synthetic sewage water at an organic load rate of 1.0 kg COD/(m(3)·d), varying the dissolved oxygen (DO) and temperature.
24679094	5	13	theme	SRT	1014:1016	arg1	20°C					989:992	20°C	989:992	20°C	989:992	The highest content of intracellular proteins in the activated sludge was 87.8 mg/g VSS, obtained at 20°C, 8 mg/L of DO and a SRT of 6 days.
24679094	5	13	theme	SRT	1014:1016	arg1	mg/L					997:1000	8 mg/L	995:1000	8 mg/L of DO and a SRT of 6 days	995:1026	The highest content of intracellular proteins in the activated sludge was 87.8 mg/g VSS, obtained at 20°C, 8 mg/L of DO and a SRT of 6 days.
24679094	2	14	theme	days	618:621	arg1	time					602:605	a sludge retention time	583:605	a sludge retention time (SRT) of 6 days	583:621	Six DO concentrations (0.8, 1.0, 1.5, 2.0, 2.5 and 8 mg/L) were studied at 20°C with a sludge retention time (SRT) of 6 days.
24679094	2	14	theme	days	618:621	arg1	SRT					608:610	SRT	608:610	SRT	608:610	Six DO concentrations (0.8, 1.0, 1.5, 2.0, 2.5 and 8 mg/L) were studied at 20°C with a sludge retention time (SRT) of 6 days.
24679094	6	15	theme	highest	1033:1039	arg1	content					1041:1047	The highest content	1029:1047	The highest content of intracellular polysaccharides in the activated sludge	1029:1104	The highest content of intracellular polysaccharides in the activated sludge was 76.6 mg/g VSS, which was achieved at 20°C, a SRT of 6 days and a wide range of DO.
24679094	6	15	theme	highest	1033:1039	arg1	VSS					1120:1122	76.6 mg/g VSS	1110:1122	76.6 mg/g VSS	1110:1122	The highest content of intracellular polysaccharides in the activated sludge was 76.6 mg/g VSS, which was achieved at 20°C, a SRT of 6 days and a wide range of DO.
24679094	6	16	theme	DO	1189:1190	arg1	range					1180:1184	a wide range	1173:1184	a wide range of DO	1173:1190	The highest content of intracellular polysaccharides in the activated sludge was 76.6 mg/g VSS, which was achieved at 20°C, a SRT of 6 days and a wide range of DO.
24679094	6	16	theme	DO	1189:1190	arg1	20°C					1147:1150	20°C	1147:1150	20°C	1147:1150	The highest content of intracellular polysaccharides in the activated sludge was 76.6 mg/g VSS, which was achieved at 20°C, a SRT of 6 days and a wide range of DO.
24679094	6	16	theme	DO	1189:1190	arg1	SRT					1155:1157	a SRT	1153:1157	a SRT of 6 days	1153:1167	The highest content of intracellular polysaccharides in the activated sludge was 76.6 mg/g VSS, which was achieved at 20°C, a SRT of 6 days and a wide range of DO.
24679094	6	17	theme	polysaccharides	1066:1080	arg1	content					1041:1047	The highest content	1029:1047	The highest content of intracellular polysaccharides in the activated sludge	1029:1104	The highest content of intracellular polysaccharides in the activated sludge was 76.6 mg/g VSS, which was achieved at 20°C, a SRT of 6 days and a wide range of DO.
24679094	6	17	theme	polysaccharides	1066:1080	arg1	VSS					1120:1122	76.6 mg/g VSS	1110:1122	76.6 mg/g VSS	1110:1122	The highest content of intracellular polysaccharides in the activated sludge was 76.6 mg/g VSS, which was achieved at 20°C, a SRT of 6 days and a wide range of DO.
24679094	0	18	theme	sludge	102:107	arg1	composition					61:71	macromolecular composition	46:71	macromolecular composition	46:71	Effect of dissolved oxygen and temperature on macromolecular composition and PHB storage of activated sludge.
24679094	0	18	theme	sludge	102:107	arg1	storage					81:87	PHB storage	77:87	PHB storage	77:87	Effect of dissolved oxygen and temperature on macromolecular composition and PHB storage of activated sludge.
24679094	1	19	theme	synthetic	376:384	arg1	water					393:397	synthetic sewage water	376:397	synthetic sewage water at an organic load rate of 1.0 kg COD/(m(3)·d)	376:444	The macromolecular composition of activated sludge (lipids, intracellular proteins and intracellular polysaccharides) was studied together with its capacity to store macromolecules such as polyhydroxybutyrate (PHB) in a conventional activated sludge system fed with synthetic sewage water at an organic load rate of 1.0 kg COD/(m(3)·d), varying the dissolved oxygen (DO) and temperature.
24679094	6	20	theme	76.6	1110:1113	arg1	mg/g					1115:1118	mg/g	1115:1118	mg/g	1115:1118	The highest content of intracellular polysaccharides in the activated sludge was 76.6 mg/g VSS, which was achieved at 20°C, a SRT of 6 days and a wide range of DO.
24679094	4	21	theme	activated	800:808	arg1	sludge					810:815	the activated sludge	796:815	the activated sludge	796:815	The highest lipid content in the activated sludge was 95.6 mg/g VSS, obtained at 30°C, 2 mg/L of DO and a SRT of 2 days.
24679094	5	22	theme	days	1023:1026	arg1	SRT					1014:1016	a SRT	1012:1016	a SRT of 6 days	1012:1026	The highest content of intracellular proteins in the activated sludge was 87.8 mg/g VSS, obtained at 20°C, 8 mg/L of DO and a SRT of 6 days.
24679094	1	23	theme	intracellular	170:182	arg1	proteins					184:191	intracellular proteins	170:191	intracellular proteins	170:191	The macromolecular composition of activated sludge (lipids, intracellular proteins and intracellular polysaccharides) was studied together with its capacity to store macromolecules such as polyhydroxybutyrate (PHB) in a conventional activated sludge system fed with synthetic sewage water at an organic load rate of 1.0 kg COD/(m(3)·d), varying the dissolved oxygen (DO) and temperature.
24679094	1	24	theme	sewage	386:391	arg1	water					393:397	synthetic sewage water	376:397	synthetic sewage water at an organic load rate of 1.0 kg COD/(m(3)·d)	376:444	The macromolecular composition of activated sludge (lipids, intracellular proteins and intracellular polysaccharides) was studied together with its capacity to store macromolecules such as polyhydroxybutyrate (PHB) in a conventional activated sludge system fed with synthetic sewage water at an organic load rate of 1.0 kg COD/(m(3)·d), varying the dissolved oxygen (DO) and temperature.
24679094	5	25	from	content	900:906	arg1	sludge					951:956	the activated sludge	937:956	the activated sludge	937:956	The highest content of intracellular proteins in the activated sludge was 87.8 mg/g VSS, obtained at 20°C, 8 mg/L of DO and a SRT of 6 days.
24679094	6	26	theme	wide	1175:1178	arg1	range					1180:1184	a wide range	1173:1184	a wide range of DO	1173:1190	The highest content of intracellular polysaccharides in the activated sludge was 76.6 mg/g VSS, which was achieved at 20°C, a SRT of 6 days and a wide range of DO.
24679094	3	27	with	constant	700:707	arg1	SRT					733:735	2 days SRT	726:735	2 days SRT	726:735	In addition, four temperatures (10ºC, 15ºC, 20ºC and 30ºC) were assessed at constant DO (2 mg/L) with 2 days SRT in a second experimental run.
24679094	3	28	dep	constant	700:707	arg1	DO					709:710	DO	709:710	DO	709:710	In addition, four temperatures (10ºC, 15ºC, 20ºC and 30ºC) were assessed at constant DO (2 mg/L) with 2 days SRT in a second experimental run.
24679094	3	29	theme	days	728:731	arg1	SRT					733:735	2 days SRT	726:735	2 days SRT	726:735	In addition, four temperatures (10ºC, 15ºC, 20ºC and 30ºC) were assessed at constant DO (2 mg/L) with 2 days SRT in a second experimental run.
24679094	0	30	theme	oxygen	20:25	arg1	Effect					0:5	Effect	0:5	Effect of dissolved oxygen and temperature on macromolecular composition and PHB storage of activated sludge.	0:108	Effect of dissolved oxygen and temperature on macromolecular composition and PHB storage of activated sludge.
24679094	6	31	theme	intracellular	1052:1064	arg1	polysaccharides					1066:1080	intracellular polysaccharides	1052:1080	intracellular polysaccharides	1052:1080	The highest content of intracellular polysaccharides in the activated sludge was 76.6 mg/g VSS, which was achieved at 20°C, a SRT of 6 days and a wide range of DO.
24679094	6	32	from	content	1041:1047	arg1	sludge					1099:1104	the activated sludge	1085:1104	the activated sludge	1085:1104	The highest content of intracellular polysaccharides in the activated sludge was 76.6 mg/g VSS, which was achieved at 20°C, a SRT of 6 days and a wide range of DO.
24679094	1	33	theme	intracellular	197:209	arg1	polysaccharides					211:225	intracellular polysaccharides	197:225	intracellular polysaccharides	197:225	The macromolecular composition of activated sludge (lipids, intracellular proteins and intracellular polysaccharides) was studied together with its capacity to store macromolecules such as polyhydroxybutyrate (PHB) in a conventional activated sludge system fed with synthetic sewage water at an organic load rate of 1.0 kg COD/(m(3)·d), varying the dissolved oxygen (DO) and temperature.
24679094	0	34	theme	dissolved	10:18	arg1	oxygen					20:25	oxygen	20:25	oxygen	20:25	Effect of dissolved oxygen and temperature on macromolecular composition and PHB storage of activated sludge.
24679094	5	35	theme	intracellular	911:923	arg1	proteins					925:932	intracellular proteins	911:932	intracellular proteins	911:932	The highest content of intracellular proteins in the activated sludge was 87.8 mg/g VSS, obtained at 20°C, 8 mg/L of DO and a SRT of 6 days.
24679094	4	36	theme	DO	864:865	arg1	30°C					848:851	30°C	848:851	30°C	848:851	The highest lipid content in the activated sludge was 95.6 mg/g VSS, obtained at 30°C, 2 mg/L of DO and a SRT of 2 days.
24679094	4	36	theme	DO	864:865	arg1	mg/L					856:859	2 mg/L	854:859	2 mg/L of DO	854:865	The highest lipid content in the activated sludge was 95.6 mg/g VSS, obtained at 30°C, 2 mg/L of DO and a SRT of 2 days.
24679094	4	36	theme	DO	864:865	arg1	SRT					873:875	a SRT	871:875	a SRT of 2 days	871:885	The highest lipid content in the activated sludge was 95.6 mg/g VSS, obtained at 30°C, 2 mg/L of DO and a SRT of 2 days.
24679094	6	37	theme	days	1164:1167	arg1	range					1180:1184	a wide range	1173:1184	a wide range of DO	1173:1190	The highest content of intracellular polysaccharides in the activated sludge was 76.6 mg/g VSS, which was achieved at 20°C, a SRT of 6 days and a wide range of DO.
24679094	6	37	theme	days	1164:1167	arg1	20°C					1147:1150	20°C	1147:1150	20°C	1147:1150	The highest content of intracellular polysaccharides in the activated sludge was 76.6 mg/g VSS, which was achieved at 20°C, a SRT of 6 days and a wide range of DO.
24679094	6	37	theme	days	1164:1167	arg1	SRT					1155:1157	a SRT	1153:1157	a SRT of 6 days	1153:1167	The highest content of intracellular polysaccharides in the activated sludge was 76.6 mg/g VSS, which was achieved at 20°C, a SRT of 6 days and a wide range of DO.
24679094	5	38	theme	DO	1005:1006	arg1	20°C					989:992	20°C	989:992	20°C	989:992	The highest content of intracellular proteins in the activated sludge was 87.8 mg/g VSS, obtained at 20°C, 8 mg/L of DO and a SRT of 6 days.
24679094	5	38	theme	DO	1005:1006	arg1	mg/L					997:1000	8 mg/L	995:1000	8 mg/L of DO and a SRT of 6 days	995:1026	The highest content of intracellular proteins in the activated sludge was 87.8 mg/g VSS, obtained at 20°C, 8 mg/L of DO and a SRT of 6 days.
24679094	0	39	theme	temperature	31:41	arg1	Effect					0:5	Effect	0:5	Effect of dissolved oxygen and temperature on macromolecular composition and PHB storage of activated sludge.	0:108	Effect of dissolved oxygen and temperature on macromolecular composition and PHB storage of activated sludge.
24679094	5	40	theme	proteins	925:932	arg1	content					900:906	The highest content	888:906	The highest content of intracellular proteins in the activated sludge	888:956	The highest content of intracellular proteins in the activated sludge was 87.8 mg/g VSS, obtained at 20°C, 8 mg/L of DO and a SRT of 6 days.
24679094	5	40	theme	proteins	925:932	arg1	VSS					972:974	87.8 mg/g VSS	962:974	87.8 mg/g VSS	962:974	The highest content of intracellular proteins in the activated sludge was 87.8 mg/g VSS, obtained at 20°C, 8 mg/L of DO and a SRT of 6 days.
24679094	3	41	dep	temperatures	642:653	arg1	temperatures					642:653	four temperatures	637:653	four temperatures (10ºC, 15ºC, 20ºC and 30ºC)	637:681	In addition, four temperatures (10ºC, 15ºC, 20ºC and 30ºC) were assessed at constant DO (2 mg/L) with 2 days SRT in a second experimental run.
24679094	3	41	dep	temperatures	642:653	arg1	15ºC					662:665	15ºC	662:665	15ºC	662:665	In addition, four temperatures (10ºC, 15ºC, 20ºC and 30ºC) were assessed at constant DO (2 mg/L) with 2 days SRT in a second experimental run.
24679094	3	41	dep	temperatures	642:653	arg1	10ºC					656:659	10ºC	656:659	10ºC	656:659	In addition, four temperatures (10ºC, 15ºC, 20ºC and 30ºC) were assessed at constant DO (2 mg/L) with 2 days SRT in a second experimental run.
24679094	3	41	dep	temperatures	642:653	arg1	30ºC					677:680	30ºC	677:680	30ºC	677:680	In addition, four temperatures (10ºC, 15ºC, 20ºC and 30ºC) were assessed at constant DO (2 mg/L) with 2 days SRT in a second experimental run.
24679094	3	41	dep	temperatures	642:653	arg1	20ºC					668:671	20ºC	668:671	20ºC	668:671	In addition, four temperatures (10ºC, 15ºC, 20ºC and 30ºC) were assessed at constant DO (2 mg/L) with 2 days SRT in a second experimental run.
24679094	1	42	dep	oxygen	469:474	arg1	DO					477:478	DO	477:478	DO	477:478	The macromolecular composition of activated sludge (lipids, intracellular proteins and intracellular polysaccharides) was studied together with its capacity to store macromolecules such as polyhydroxybutyrate (PHB) in a conventional activated sludge system fed with synthetic sewage water at an organic load rate of 1.0 kg COD/(m(3)·d), varying the dissolved oxygen (DO) and temperature.
24679094	4	43	theme	lipid	779:783	arg1	content					785:791	The highest lipid content	767:791	The highest lipid content in the activated sludge	767:815	The highest lipid content in the activated sludge was 95.6 mg/g VSS, obtained at 30°C, 2 mg/L of DO and a SRT of 2 days.
24679094	4	43	theme	lipid	779:783	arg1	VSS					831:833	95.6 mg/g VSS	821:833	95.6 mg/g VSS	821:833	The highest lipid content in the activated sludge was 95.6 mg/g VSS, obtained at 30°C, 2 mg/L of DO and a SRT of 2 days.
24679094	1	44	dep	studied	232:238	arg1	proteins					184:191	intracellular proteins	170:191	intracellular proteins	170:191	The macromolecular composition of activated sludge (lipids, intracellular proteins and intracellular polysaccharides) was studied together with its capacity to store macromolecules such as polyhydroxybutyrate (PHB) in a conventional activated sludge system fed with synthetic sewage water at an organic load rate of 1.0 kg COD/(m(3)·d), varying the dissolved oxygen (DO) and temperature.
24679094	1	44	dep	studied	232:238	arg1	polysaccharides					211:225	intracellular polysaccharides	197:225	intracellular polysaccharides	197:225	The macromolecular composition of activated sludge (lipids, intracellular proteins and intracellular polysaccharides) was studied together with its capacity to store macromolecules such as polyhydroxybutyrate (PHB) in a conventional activated sludge system fed with synthetic sewage water at an organic load rate of 1.0 kg COD/(m(3)·d), varying the dissolved oxygen (DO) and temperature.
24679094	1	44	dep	studied	232:238	arg1	lipids					162:167	lipids	162:167	lipids	162:167	The macromolecular composition of activated sludge (lipids, intracellular proteins and intracellular polysaccharides) was studied together with its capacity to store macromolecules such as polyhydroxybutyrate (PHB) in a conventional activated sludge system fed with synthetic sewage water at an organic load rate of 1.0 kg COD/(m(3)·d), varying the dissolved oxygen (DO) and temperature.
24679094	4	45	theme	95.6	821:824	arg1	mg/g					826:829	mg/g	826:829	mg/g	826:829	The highest lipid content in the activated sludge was 95.6 mg/g VSS, obtained at 30°C, 2 mg/L of DO and a SRT of 2 days.
24679094	2	46	theme	sludge	585:590	arg1	time					602:605	a sludge retention time	583:605	a sludge retention time (SRT) of 6 days	583:621	Six DO concentrations (0.8, 1.0, 1.5, 2.0, 2.5 and 8 mg/L) were studied at 20°C with a sludge retention time (SRT) of 6 days.
24679094	2	46	theme	sludge	585:590	arg1	SRT					608:610	SRT	608:610	SRT	608:610	Six DO concentrations (0.8, 1.0, 1.5, 2.0, 2.5 and 8 mg/L) were studied at 20°C with a sludge retention time (SRT) of 6 days.
24679094	0	47	theme	macromolecular	46:59	arg1	composition					61:71	macromolecular composition	46:71	macromolecular composition	46:71	Effect of dissolved oxygen and temperature on macromolecular composition and PHB storage of activated sludge.
24679094	1	48	theme	organic	405:411	arg1	rate					418:421	an organic load rate	402:421	an organic load rate of 1.0 kg COD/(m(3)·d)	402:444	The macromolecular composition of activated sludge (lipids, intracellular proteins and intracellular polysaccharides) was studied together with its capacity to store macromolecules such as polyhydroxybutyrate (PHB) in a conventional activated sludge system fed with synthetic sewage water at an organic load rate of 1.0 kg COD/(m(3)·d), varying the dissolved oxygen (DO) and temperature.
24679094	3	49	theme	experimental	749:760	arg1	run					762:764	a second experimental run	740:764	a second experimental run	740:764	In addition, four temperatures (10ºC, 15ºC, 20ºC and 30ºC) were assessed at constant DO (2 mg/L) with 2 days SRT in a second experimental run.
24679094	6	50	theme	activated	1089:1097	arg1	sludge					1099:1104	the activated sludge	1085:1104	the activated sludge	1085:1104	The highest content of intracellular polysaccharides in the activated sludge was 76.6 mg/g VSS, which was achieved at 20°C, a SRT of 6 days and a wide range of DO.
24679094	4	51	from	content	785:791	arg1	sludge					810:815	the activated sludge	796:815	the activated sludge	796:815	The highest lipid content in the activated sludge was 95.6 mg/g VSS, obtained at 30°C, 2 mg/L of DO and a SRT of 2 days.
24679094	1	52	theme	load	413:416	arg1	rate					418:421	an organic load rate	402:421	an organic load rate of 1.0 kg COD/(m(3)·d)	402:444	The macromolecular composition of activated sludge (lipids, intracellular proteins and intracellular polysaccharides) was studied together with its capacity to store macromolecules such as polyhydroxybutyrate (PHB) in a conventional activated sludge system fed with synthetic sewage water at an organic load rate of 1.0 kg COD/(m(3)·d), varying the dissolved oxygen (DO) and temperature.
24679094	1	53	theme	macromolecular	114:127	arg1	composition					129:139	The macromolecular composition	110:139	The macromolecular composition of activated sludge	110:159	The macromolecular composition of activated sludge (lipids, intracellular proteins and intracellular polysaccharides) was studied together with its capacity to store macromolecules such as polyhydroxybutyrate (PHB) in a conventional activated sludge system fed with synthetic sewage water at an organic load rate of 1.0 kg COD/(m(3)·d), varying the dissolved oxygen (DO) and temperature.
24679094	5	54	theme	highest	892:898	arg1	content					900:906	The highest content	888:906	The highest content of intracellular proteins in the activated sludge	888:956	The highest content of intracellular proteins in the activated sludge was 87.8 mg/g VSS, obtained at 20°C, 8 mg/L of DO and a SRT of 6 days.
24679094	5	54	theme	highest	892:898	arg1	VSS					972:974	87.8 mg/g VSS	962:974	87.8 mg/g VSS	962:974	The highest content of intracellular proteins in the activated sludge was 87.8 mg/g VSS, obtained at 20°C, 8 mg/L of DO and a SRT of 6 days.
24679094	7	55	theme	PHB	1214:1216	arg1	storage					1218:1224	The activated sludge PHB storage	1193:1224	The activated sludge PHB storage	1193:1224	The activated sludge PHB storage was very low for all the conditions studied.
24679094	7	55	theme	PHB	1214:1216	arg1	low					1235:1237	low	1235:1237	low	1235:1237	The activated sludge PHB storage was very low for all the conditions studied.
24679094	3	56	theme	second	742:747	arg1	run					762:764	a second experimental run	740:764	a second experimental run	740:764	In addition, four temperatures (10ºC, 15ºC, 20ºC and 30ºC) were assessed at constant DO (2 mg/L) with 2 days SRT in a second experimental run.
24679094	1	57	theme	dissolved	459:467	arg1	oxygen					469:474	the dissolved oxygen	455:474	the dissolved oxygen (DO)	455:479	The macromolecular composition of activated sludge (lipids, intracellular proteins and intracellular polysaccharides) was studied together with its capacity to store macromolecules such as polyhydroxybutyrate (PHB) in a conventional activated sludge system fed with synthetic sewage water at an organic load rate of 1.0 kg COD/(m(3)·d), varying the dissolved oxygen (DO) and temperature.
24679094	5	58	theme	87.8	962:965	arg1	mg/g					967:970	mg/g	967:970	mg/g	967:970	The highest content of intracellular proteins in the activated sludge was 87.8 mg/g VSS, obtained at 20°C, 8 mg/L of DO and a SRT of 6 days.
24679094	4	59	theme	days	882:885	arg1	30°C					848:851	30°C	848:851	30°C	848:851	The highest lipid content in the activated sludge was 95.6 mg/g VSS, obtained at 30°C, 2 mg/L of DO and a SRT of 2 days.
24679094	4	59	theme	days	882:885	arg1	mg/L					856:859	2 mg/L	854:859	2 mg/L of DO	854:865	The highest lipid content in the activated sludge was 95.6 mg/g VSS, obtained at 30°C, 2 mg/L of DO and a SRT of 2 days.
24679094	4	59	theme	days	882:885	arg1	SRT					873:875	a SRT	871:875	a SRT of 2 days	871:885	The highest lipid content in the activated sludge was 95.6 mg/g VSS, obtained at 30°C, 2 mg/L of DO and a SRT of 2 days.
24679094	1	60	theme	activated	144:152	arg1	sludge					154:159	activated sludge	144:159	activated sludge	144:159	The macromolecular composition of activated sludge (lipids, intracellular proteins and intracellular polysaccharides) was studied together with its capacity to store macromolecules such as polyhydroxybutyrate (PHB) in a conventional activated sludge system fed with synthetic sewage water at an organic load rate of 1.0 kg COD/(m(3)·d), varying the dissolved oxygen (DO) and temperature.
24679094	5	61	theme	mg/g	967:970	arg1	content					900:906	The highest content	888:906	The highest content of intracellular proteins in the activated sludge	888:956	The highest content of intracellular proteins in the activated sludge was 87.8 mg/g VSS, obtained at 20°C, 8 mg/L of DO and a SRT of 6 days.
24679094	5	61	theme	mg/g	967:970	arg1	VSS					972:974	87.8 mg/g VSS	962:974	87.8 mg/g VSS	962:974	The highest content of intracellular proteins in the activated sludge was 87.8 mg/g VSS, obtained at 20°C, 8 mg/L of DO and a SRT of 6 days.
24679094	5	62	theme	activated	941:949	arg1	sludge					951:956	the activated sludge	937:956	the activated sludge	937:956	The highest content of intracellular proteins in the activated sludge was 87.8 mg/g VSS, obtained at 20°C, 8 mg/L of DO and a SRT of 6 days.
24679094	1	63	theme	conventional	330:341	arg1	system					360:365	a conventional activated sludge system	328:365	a conventional activated sludge system fed with synthetic sewage water at an organic load rate of 1.0 kg COD/(m(3)·d)	328:444	The macromolecular composition of activated sludge (lipids, intracellular proteins and intracellular polysaccharides) was studied together with its capacity to store macromolecules such as polyhydroxybutyrate (PHB) in a conventional activated sludge system fed with synthetic sewage water at an organic load rate of 1.0 kg COD/(m(3)·d), varying the dissolved oxygen (DO) and temperature.
24679094	6	64	theme	mg/g	1115:1118	arg1	content					1041:1047	The highest content	1029:1047	The highest content of intracellular polysaccharides in the activated sludge	1029:1104	The highest content of intracellular polysaccharides in the activated sludge was 76.6 mg/g VSS, which was achieved at 20°C, a SRT of 6 days and a wide range of DO.
24679094	6	64	theme	mg/g	1115:1118	arg1	VSS					1120:1122	76.6 mg/g VSS	1110:1122	76.6 mg/g VSS	1110:1122	The highest content of intracellular polysaccharides in the activated sludge was 76.6 mg/g VSS, which was achieved at 20°C, a SRT of 6 days and a wide range of DO.
24679094	1	65	from	rate	418:421	arg1	water					393:397	synthetic sewage water	376:397	synthetic sewage water at an organic load rate of 1.0 kg COD/(m(3)·d)	376:444	The macromolecular composition of activated sludge (lipids, intracellular proteins and intracellular polysaccharides) was studied together with its capacity to store macromolecules such as polyhydroxybutyrate (PHB) in a conventional activated sludge system fed with synthetic sewage water at an organic load rate of 1.0 kg COD/(m(3)·d), varying the dissolved oxygen (DO) and temperature.
24679094	1	66	theme	sludge	154:159	arg1	composition					129:139	The macromolecular composition	110:139	The macromolecular composition of activated sludge	110:159	The macromolecular composition of activated sludge (lipids, intracellular proteins and intracellular polysaccharides) was studied together with its capacity to store macromolecules such as polyhydroxybutyrate (PHB) in a conventional activated sludge system fed with synthetic sewage water at an organic load rate of 1.0 kg COD/(m(3)·d), varying the dissolved oxygen (DO) and temperature.
24507261	0	0	theme	gastric	95:101	arg1	cancer					103:108	human gastric cancer	89:108	human gastric cancer	89:108	Panax ginseng polysaccharide induces apoptosis by targeting Twist/AKR1C2/NF-1 pathway in human gastric cancer.
24507261	3	1	theme	molecular	323:331	arg1	weight					333:338	The average molecular weight	311:338	The average molecular weight	311:338	The average molecular weight was estimated to be 3.2 × 10(4)Da.
24507261	3	1	theme	molecular	323:331	arg1	Da					371:372	3.2 × 10(4)Da	360:372	3.2 × 10(4)Da	360:372	The average molecular weight was estimated to be 3.2 × 10(4)Da.
24507261	0	2	theme	human	89:93	arg1	cancer					103:108	human gastric cancer	89:108	human gastric cancer	89:108	Panax ginseng polysaccharide induces apoptosis by targeting Twist/AKR1C2/NF-1 pathway in human gastric cancer.
24507261	7	3	theme	protein	1004:1010	arg1	level					1012:1016	protein level	1004:1016	protein level	1004:1016	Moreover, western blotting analysis showed that protein expressions of Twist and AKR1C2 were suppressed by PGP2a, whereas an increase of NF1 was observed at protein level.
24507261	6	4	theme	increasing	835:844	arg1	concentration					821:833	concentration increasing	821:844	concentration increasing	821:844	Furthermore, the number of HGC-27 cells arrested in G2/M phase, and the percentage of apoptotic cells were increased in response to PGP2a treatment along with concentration increasing.
24507261	2	5	theme	acidic	280:285	arg1	PGP2a					267:271	PGP2a	267:271	PGP2a	267:271	Chemical composition analysis indicated PGP2a was an acidic protein-polysaccharide.
24507261	2	5	theme	acidic	280:285	arg1	protein-polysaccharide					287:308	an acidic protein-polysaccharide	277:308	an acidic protein-polysaccharide	277:308	Chemical composition analysis indicated PGP2a was an acidic protein-polysaccharide.
24507261	7	6	theme	western	857:863	arg1	analysis					874:881	western blotting analysis	857:881	western blotting analysis	857:881	Moreover, western blotting analysis showed that protein expressions of Twist and AKR1C2 were suppressed by PGP2a, whereas an increase of NF1 was observed at protein level.
24507261	3	7	theme	×	364:364	arg1	weight					333:338	The average molecular weight	311:338	The average molecular weight	311:338	The average molecular weight was estimated to be 3.2 × 10(4)Da.
24507261	3	7	theme	×	364:364	arg1	Da					371:372	3.2 × 10(4)Da	360:372	3.2 × 10(4)Da	360:372	The average molecular weight was estimated to be 3.2 × 10(4)Da.
24507261	6	8	theme	cells	696:700	arg1	number					679:684	the number	675:684	the number of HGC-27 cells arrested in G2/M phase	675:723	Furthermore, the number of HGC-27 cells arrested in G2/M phase, and the percentage of apoptotic cells were increased in response to PGP2a treatment along with concentration increasing.
24507261	6	8	theme	cells	696:700	arg1	percentage					734:743	the percentage	730:743	the percentage of apoptotic cells	730:762	Furthermore, the number of HGC-27 cells arrested in G2/M phase, and the percentage of apoptotic cells were increased in response to PGP2a treatment along with concentration increasing.
24507261	5	9	contain	had	572:574	arg2	effect					596:601	a potent inhibitory effect	576:601	a potent inhibitory effect	576:601	MTT assay showed that PGP2a had a potent inhibitory effect on the growth of HGC-27 cells in a dose-dependent fashion.
24507261	5	9	contain	had	572:574	arg1	PGP2a					566:570	PGP2a	566:570	PGP2a	566:570	MTT assay showed that PGP2a had a potent inhibitory effect on the growth of HGC-27 cells in a dose-dependent fashion.
24507261	7	10	theme	protein	895:901	arg1	expressions					903:913	protein expressions	895:913	protein expressions of Twist and AKR1C2	895:933	Moreover, western blotting analysis showed that protein expressions of Twist and AKR1C2 were suppressed by PGP2a, whereas an increase of NF1 was observed at protein level.
24507261	1	11	theme	antitumor	161:169	arg1	PGP2a					187:191	PGP2a	187:191	PGP2a	187:191	In the present study, we isolated and screened an antitumor polysaccharide (PGP2a) from the roots of Panax ginseng.
24507261	1	11	theme	antitumor	161:169	arg1	polysaccharide					171:184	an antitumor polysaccharide	158:184	an antitumor polysaccharide (PGP2a) from the roots of Panax ginseng	158:224	In the present study, we isolated and screened an antitumor polysaccharide (PGP2a) from the roots of Panax ginseng.
24507261	6	12	dep	treatment	800:808	arg1	response					782:789	response	782:789	response	782:789	Furthermore, the number of HGC-27 cells arrested in G2/M phase, and the percentage of apoptotic cells were increased in response to PGP2a treatment along with concentration increasing.
24507261	0	13	theme	ginseng	6:12	arg1	polysaccharide					14:27	Panax ginseng polysaccharide	0:27	Panax ginseng polysaccharide	0:27	Panax ginseng polysaccharide induces apoptosis by targeting Twist/AKR1C2/NF-1 pathway in human gastric cancer.
24507261	5	14	theme	potent	578:583	arg1	effect					596:601	a potent inhibitory effect	576:601	a potent inhibitory effect	576:601	MTT assay showed that PGP2a had a potent inhibitory effect on the growth of HGC-27 cells in a dose-dependent fashion.
24507261	5	15	theme	HGC-27	620:625	arg1	cells					627:631	HGC-27 cells	620:631	HGC-27 cells	620:631	MTT assay showed that PGP2a had a potent inhibitory effect on the growth of HGC-27 cells in a dose-dependent fashion.
24507261	8	16	theme	cancer	1164:1169	arg1	therapy					1171:1177	human gastric cancer therapy	1150:1177	human gastric cancer therapy	1150:1177	Taken together, these findings suggested that PGP2a could be developed as a novel antitumor agent acting on Twist related gene for human gastric cancer therapy.
24507261	0	17	theme	Panax	0:4	arg1	polysaccharide					14:27	Panax ginseng polysaccharide	0:27	Panax ginseng polysaccharide	0:27	Panax ginseng polysaccharide induces apoptosis by targeting Twist/AKR1C2/NF-1 pathway in human gastric cancer.
24507261	5	18	theme	inhibitory	585:594	arg1	effect					596:601	a potent inhibitory effect	576:601	a potent inhibitory effect	576:601	MTT assay showed that PGP2a had a potent inhibitory effect on the growth of HGC-27 cells in a dose-dependent fashion.
24507261	6	19	theme	G2/M	714:717	arg1	phase					719:723	G2/M phase	714:723	G2/M phase	714:723	Furthermore, the number of HGC-27 cells arrested in G2/M phase, and the percentage of apoptotic cells were increased in response to PGP2a treatment along with concentration increasing.
24507261	8	20	theme	gastric	1156:1162	arg1	therapy					1171:1177	human gastric cancer therapy	1150:1177	human gastric cancer therapy	1150:1177	Taken together, these findings suggested that PGP2a could be developed as a novel antitumor agent acting on Twist related gene for human gastric cancer therapy.
24507261	4	21	theme	molar	498:502	arg1	ratio					504:508	the molar ratio	494:508	the molar ratio of 3.7:1.6:0.5:5.4	494:527	According to gas chromatography (GC) result, PGP2a consisted of galactose, arabinose, glucose and galacturonic acid in the molar ratio of 3.7:1.6:0.5:5.4, respectively.
24507261	5	22	theme	MTT	544:546	arg1	assay					548:552	MTT assay	544:552	MTT assay	544:552	MTT assay showed that PGP2a had a potent inhibitory effect on the growth of HGC-27 cells in a dose-dependent fashion.
24507261	7	23	located	observed	992:999	arg1	level					1012:1016	protein level	1004:1016	protein level	1004:1016	Moreover, western blotting analysis showed that protein expressions of Twist and AKR1C2 were suppressed by PGP2a, whereas an increase of NF1 was observed at protein level.
24507261	7	23	located	observed	992:999	arg2	increase					972:979	an increase	969:979	an increase of NF1	969:986	Moreover, western blotting analysis showed that protein expressions of Twist and AKR1C2 were suppressed by PGP2a, whereas an increase of NF1 was observed at protein level.
24507261	4	24	theme	3.7:1.6:0.5:5.4	513:527	arg1	ratio					504:508	the molar ratio	494:508	the molar ratio of 3.7:1.6:0.5:5.4	494:527	According to gas chromatography (GC) result, PGP2a consisted of galactose, arabinose, glucose and galacturonic acid in the molar ratio of 3.7:1.6:0.5:5.4, respectively.
24507261	5	25	theme	dose-dependent	638:651	arg1	fashion					653:659	a dose-dependent fashion	636:659	a dose-dependent fashion	636:659	MTT assay showed that PGP2a had a potent inhibitory effect on the growth of HGC-27 cells in a dose-dependent fashion.
24507261	6	26	theme	PGP2a	794:798	arg1	treatment					800:808	PGP2a treatment	794:808	PGP2a treatment	794:808	Furthermore, the number of HGC-27 cells arrested in G2/M phase, and the percentage of apoptotic cells were increased in response to PGP2a treatment along with concentration increasing.
24507261	8	27	theme	Twist	1127:1131	arg1	gene					1141:1144	Twist related gene	1127:1144	Twist related gene for human gastric cancer therapy	1127:1177	Taken together, these findings suggested that PGP2a could be developed as a novel antitumor agent acting on Twist related gene for human gastric cancer therapy.
24507261	7	28	theme	NF1	984:986	arg1	increase					972:979	an increase	969:979	an increase of NF1	969:986	Moreover, western blotting analysis showed that protein expressions of Twist and AKR1C2 were suppressed by PGP2a, whereas an increase of NF1 was observed at protein level.
24507261	8	29	theme	human	1150:1154	arg1	therapy					1171:1177	human gastric cancer therapy	1150:1177	human gastric cancer therapy	1150:1177	Taken together, these findings suggested that PGP2a could be developed as a novel antitumor agent acting on Twist related gene for human gastric cancer therapy.
24507261	0	30	theme	Twist/AKR1C2/NF-1	60:76	arg1	pathway					78:84	Twist/AKR1C2/NF-1 pathway	60:84	Twist/AKR1C2/NF-1 pathway in human gastric cancer	60:108	Panax ginseng polysaccharide induces apoptosis by targeting Twist/AKR1C2/NF-1 pathway in human gastric cancer.
24507261	4	31	theme	galacturonic	473:484	arg1	acid					486:489	galacturonic acid	473:489	galacturonic acid	473:489	According to gas chromatography (GC) result, PGP2a consisted of galactose, arabinose, glucose and galacturonic acid in the molar ratio of 3.7:1.6:0.5:5.4, respectively.
24507261	0	32	from	pathway	78:84	arg1	cancer					103:108	human gastric cancer	89:108	human gastric cancer	89:108	Panax ginseng polysaccharide induces apoptosis by targeting Twist/AKR1C2/NF-1 pathway in human gastric cancer.
24507261	1	33	from	roots	203:207	arg1	PGP2a					187:191	PGP2a	187:191	PGP2a	187:191	In the present study, we isolated and screened an antitumor polysaccharide (PGP2a) from the roots of Panax ginseng.
24507261	1	33	from	roots	203:207	arg1	polysaccharide					171:184	an antitumor polysaccharide	158:184	an antitumor polysaccharide (PGP2a) from the roots of Panax ginseng	158:224	In the present study, we isolated and screened an antitumor polysaccharide (PGP2a) from the roots of Panax ginseng.
24507261	1	34	theme	present	118:124	arg1	study					126:130	the present study	114:130	the present study	114:130	In the present study, we isolated and screened an antitumor polysaccharide (PGP2a) from the roots of Panax ginseng.
24507261	8	35	theme	related	1133:1139	arg1	gene					1141:1144	Twist related gene	1127:1144	Twist related gene for human gastric cancer therapy	1127:1177	Taken together, these findings suggested that PGP2a could be developed as a novel antitumor agent acting on Twist related gene for human gastric cancer therapy.
24507261	3	36	theme	average	315:321	arg1	weight					333:338	The average molecular weight	311:338	The average molecular weight	311:338	The average molecular weight was estimated to be 3.2 × 10(4)Da.
24507261	3	36	theme	average	315:321	arg1	Da					371:372	3.2 × 10(4)Da	360:372	3.2 × 10(4)Da	360:372	The average molecular weight was estimated to be 3.2 × 10(4)Da.
24507261	4	37	theme	chromatography	392:405	arg1	result					412:417	gas chromatography (GC) result	388:417	gas chromatography (GC) result	388:417	According to gas chromatography (GC) result, PGP2a consisted of galactose, arabinose, glucose and galacturonic acid in the molar ratio of 3.7:1.6:0.5:5.4, respectively.
24507261	6	38	theme	HGC-27	689:694	arg1	cells					696:700	HGC-27 cells	689:700	HGC-27 cells arrested in G2/M phase	689:723	Furthermore, the number of HGC-27 cells arrested in G2/M phase, and the percentage of apoptotic cells were increased in response to PGP2a treatment along with concentration increasing.
24507261	7	39	theme	blotting	865:872	arg1	analysis					874:881	western blotting analysis	857:881	western blotting analysis	857:881	Moreover, western blotting analysis showed that protein expressions of Twist and AKR1C2 were suppressed by PGP2a, whereas an increase of NF1 was observed at protein level.
24507261	5	40	theme	cells	627:631	arg1	growth					610:615	the growth	606:615	the growth of HGC-27 cells in a dose-dependent fashion	606:659	MTT assay showed that PGP2a had a potent inhibitory effect on the growth of HGC-27 cells in a dose-dependent fashion.
24507261	4	41	theme	gas	388:390	arg1	GC					408:409	GC	408:409	GC	408:409	According to gas chromatography (GC) result, PGP2a consisted of galactose, arabinose, glucose and galacturonic acid in the molar ratio of 3.7:1.6:0.5:5.4, respectively.
24507261	4	41	theme	gas	388:390	arg1	chromatography					392:405	gas chromatography	388:405	gas chromatography (GC) result	388:417	According to gas chromatography (GC) result, PGP2a consisted of galactose, arabinose, glucose and galacturonic acid in the molar ratio of 3.7:1.6:0.5:5.4, respectively.
24507261	6	42	theme	cells	758:762	arg1	number					679:684	the number	675:684	the number of HGC-27 cells arrested in G2/M phase	675:723	Furthermore, the number of HGC-27 cells arrested in G2/M phase, and the percentage of apoptotic cells were increased in response to PGP2a treatment along with concentration increasing.
24507261	6	42	theme	cells	758:762	arg1	percentage					734:743	the percentage	730:743	the percentage of apoptotic cells	730:762	Furthermore, the number of HGC-27 cells arrested in G2/M phase, and the percentage of apoptotic cells were increased in response to PGP2a treatment along with concentration increasing.
24507261	8	43	theme	novel	1095:1099	arg1	agent					1111:1115	a novel antitumor agent	1093:1115	a novel antitumor agent acting on Twist related gene for human gastric cancer therapy	1093:1177	Taken together, these findings suggested that PGP2a could be developed as a novel antitumor agent acting on Twist related gene for human gastric cancer therapy.
24507261	8	43	theme	novel	1095:1099	arg1	PGP2a					1065:1069	PGP2a	1065:1069	PGP2a	1065:1069	Taken together, these findings suggested that PGP2a could be developed as a novel antitumor agent acting on Twist related gene for human gastric cancer therapy.
24507261	7	44	theme	AKR1C2	928:933	arg1	expressions					903:913	protein expressions	895:913	protein expressions of Twist and AKR1C2	895:933	Moreover, western blotting analysis showed that protein expressions of Twist and AKR1C2 were suppressed by PGP2a, whereas an increase of NF1 was observed at protein level.
24507261	1	45	theme	Panax	212:216	arg1	ginseng					218:224	Panax ginseng	212:224	Panax ginseng	212:224	In the present study, we isolated and screened an antitumor polysaccharide (PGP2a) from the roots of Panax ginseng.
24507261	6	46	theme	apoptotic	748:756	arg1	cells					758:762	apoptotic cells	748:762	apoptotic cells	748:762	Furthermore, the number of HGC-27 cells arrested in G2/M phase, and the percentage of apoptotic cells were increased in response to PGP2a treatment along with concentration increasing.
24507261	2	47	theme	composition	236:246	arg1	analysis					248:255	Chemical composition analysis	227:255	Chemical composition analysis	227:255	Chemical composition analysis indicated PGP2a was an acidic protein-polysaccharide.
24507261	1	48	theme	ginseng	218:224	arg1	roots					203:207	the roots	199:207	the roots of Panax ginseng	199:224	In the present study, we isolated and screened an antitumor polysaccharide (PGP2a) from the roots of Panax ginseng.
24507261	2	49	theme	Chemical	227:234	arg1	analysis					248:255	Chemical composition analysis	227:255	Chemical composition analysis	227:255	Chemical composition analysis indicated PGP2a was an acidic protein-polysaccharide.
24507261	7	50	theme	Twist	918:922	arg1	expressions					903:913	protein expressions	895:913	protein expressions of Twist and AKR1C2	895:933	Moreover, western blotting analysis showed that protein expressions of Twist and AKR1C2 were suppressed by PGP2a, whereas an increase of NF1 was observed at protein level.
24507261	8	51	theme	antitumor	1101:1109	arg1	agent					1111:1115	a novel antitumor agent	1093:1115	a novel antitumor agent acting on Twist related gene for human gastric cancer therapy	1093:1177	Taken together, these findings suggested that PGP2a could be developed as a novel antitumor agent acting on Twist related gene for human gastric cancer therapy.
24507261	8	51	theme	antitumor	1101:1109	arg1	PGP2a					1065:1069	PGP2a	1065:1069	PGP2a	1065:1069	Taken together, these findings suggested that PGP2a could be developed as a novel antitumor agent acting on Twist related gene for human gastric cancer therapy.
24507261	5	52	from	growth	610:615	arg1	fashion					653:659	a dose-dependent fashion	636:659	a dose-dependent fashion	636:659	MTT assay showed that PGP2a had a potent inhibitory effect on the growth of HGC-27 cells in a dose-dependent fashion.
24686371	5	0	theme	enzyme	858:863	arg1	accessibility					865:877	enzyme accessibility	858:877	enzyme accessibility	858:877	LCNF hydrolysis rate gradually decreased with lignin and hemicellulose concentration, both of which limited enzyme accessibility.
24686371	1	1	theme	lignin	181:186	arg1	hemicelluloses					192:205	hemicelluloses	192:205	hemicelluloses	192:205	The amount of residual lignin and hemicelluloses in softwood fibers was systematically varied by SO2-ethanol-water fractionation for integrated biorefinery with nanomaterial and biofuel production.
24686371	1	1	theme	lignin	181:186	arg1	amount					162:167	The amount	158:167	The amount of residual lignin and hemicelluloses in softwood fibers	158:224	The amount of residual lignin and hemicelluloses in softwood fibers was systematically varied by SO2-ethanol-water fractionation for integrated biorefinery with nanomaterial and biofuel production.
24686371	1	1	theme	lignin	181:186	arg1	lignin					181:186	residual lignin	172:186	residual lignin	172:186	The amount of residual lignin and hemicelluloses in softwood fibers was systematically varied by SO2-ethanol-water fractionation for integrated biorefinery with nanomaterial and biofuel production.
24686371	0	2	theme	softwood	77:84	arg1	bioconversion					60:72	the bioconversion	56:72	the bioconversion of softwood	56:84	Effects of residual lignin and heteropolysaccharides on the bioconversion of softwood lignocellulose nanofibrils obtained by SO2-ethanol-water fractionation.
24686371	2	3	theme	mechanical	399:408	arg1	processing					410:419	mechanical processing	399:419	mechanical processing	399:419	On the basis of their low energy demand in mechanical processing, the fibers were deconstructed to lignocellulose nanofibrils (LCNF) and used as substrate for bioconversion.
24686371	4	4	theme	LCNF	648:651	arg1	digestibility					653:665	LCNF digestibility	648:665	LCNF digestibility	648:665	LCNF digestibility was compared with the enzyme activity measured with a quartz crystal microbalance.
24686371	0	5	from	Effects	0:6	arg1	bioconversion					60:72	the bioconversion	56:72	the bioconversion of softwood	56:84	Effects of residual lignin and heteropolysaccharides on the bioconversion of softwood lignocellulose nanofibrils obtained by SO2-ethanol-water fractionation.
24686371	3	6	theme	different	628:636	arg1	loadings					638:645	different loadings	628:645	different loadings	628:645	The effect of LCNF composition on saccharification via multicomponent enzymes was investigated at different loadings.
24686371	3	7	from	effect	534:539	arg1	saccharification					564:579	saccharification	564:579	saccharification via multicomponent enzymes	564:606	The effect of LCNF composition on saccharification via multicomponent enzymes was investigated at different loadings.
24686371	5	8	theme	LCNF	750:753	arg1	rate					766:769	LCNF hydrolysis rate	750:769	LCNF hydrolysis rate	750:769	LCNF hydrolysis rate gradually decreased with lignin and hemicellulose concentration, both of which limited enzyme accessibility.
24686371	6	9	theme	non-productive	912:925	arg1	binding					927:933	non-productive binding	912:933	non-productive binding of proteins onto lignin	912:957	Enzyme inhibition resulted from non-productive binding of proteins onto lignin.
24686371	3	10	theme	composition	549:559	arg1	effect					534:539	The effect	530:539	The effect of LCNF composition on saccharification via multicomponent enzymes	530:606	The effect of LCNF composition on saccharification via multicomponent enzymes was investigated at different loadings.
24686371	7	11	theme	LCNF	974:977	arg1	hydrolysis					979:988	Near complete LCNF hydrolysis	960:988	Near complete LCNF hydrolysis	960:988	Near complete LCNF hydrolysis was achieved, even at high lignin and hemicellulose content.
24686371	0	12	theme	SO2-ethanol-water	125:141	arg1	fractionation					143:155	SO2-ethanol-water fractionation	125:155	SO2-ethanol-water fractionation	125:155	Effects of residual lignin and heteropolysaccharides on the bioconversion of softwood lignocellulose nanofibrils obtained by SO2-ethanol-water fractionation.
24686371	8	13	theme	precursor	1100:1108	arg1	fibers					1114:1119	precursor SEW fibers	1100:1119	precursor SEW fibers	1100:1119	Sugar yields for LCNF were higher than those for precursor SEW fibers, highlighting the benefits of high surface area in LCNF.
24686371	8	14	theme	surface	1156:1162	arg1	area					1164:1167	high surface area	1151:1167	high surface area	1151:1167	Sugar yields for LCNF were higher than those for precursor SEW fibers, highlighting the benefits of high surface area in LCNF.
24686371	2	15	from	demand	389:394	arg1	processing					410:419	mechanical processing	399:419	mechanical processing	399:419	On the basis of their low energy demand in mechanical processing, the fibers were deconstructed to lignocellulose nanofibrils (LCNF) and used as substrate for bioconversion.
24686371	3	16	theme	multicomponent	585:598	arg1	enzymes					600:606	multicomponent enzymes	585:606	multicomponent enzymes	585:606	The effect of LCNF composition on saccharification via multicomponent enzymes was investigated at different loadings.
24686371	1	17	theme	hemicelluloses	192:205	arg1	hemicelluloses					192:205	hemicelluloses	192:205	hemicelluloses	192:205	The amount of residual lignin and hemicelluloses in softwood fibers was systematically varied by SO2-ethanol-water fractionation for integrated biorefinery with nanomaterial and biofuel production.
24686371	1	17	theme	hemicelluloses	192:205	arg1	amount					162:167	The amount	158:167	The amount of residual lignin and hemicelluloses in softwood fibers	158:224	The amount of residual lignin and hemicelluloses in softwood fibers was systematically varied by SO2-ethanol-water fractionation for integrated biorefinery with nanomaterial and biofuel production.
24686371	1	17	theme	hemicelluloses	192:205	arg1	lignin					181:186	residual lignin	172:186	residual lignin	172:186	The amount of residual lignin and hemicelluloses in softwood fibers was systematically varied by SO2-ethanol-water fractionation for integrated biorefinery with nanomaterial and biofuel production.
24686371	2	18	used	used	493:496	arg2	fibers					426:431	the fibers	422:431	the fibers	422:431	On the basis of their low energy demand in mechanical processing, the fibers were deconstructed to lignocellulose nanofibrils (LCNF) and used as substrate for bioconversion.
24686371	2	18	used	used	493:496	arg2	substrate					501:509	substrate	501:509	substrate	501:509	On the basis of their low energy demand in mechanical processing, the fibers were deconstructed to lignocellulose nanofibrils (LCNF) and used as substrate for bioconversion.
24686371	8	19	theme	high	1151:1154	arg1	area					1164:1167	high surface area	1151:1167	high surface area	1151:1167	Sugar yields for LCNF were higher than those for precursor SEW fibers, highlighting the benefits of high surface area in LCNF.
24686371	8	20	theme	SEW	1110:1112	arg1	fibers					1114:1119	precursor SEW fibers	1100:1119	precursor SEW fibers	1100:1119	Sugar yields for LCNF were higher than those for precursor SEW fibers, highlighting the benefits of high surface area in LCNF.
24686371	4	21	theme	crystal	728:734	arg1	microbalance					736:747	a quartz crystal microbalance	719:747	a quartz crystal microbalance	719:747	LCNF digestibility was compared with the enzyme activity measured with a quartz crystal microbalance.
24686371	1	22	from	amount	162:167	arg1	fibers					219:224	softwood fibers	210:224	softwood fibers	210:224	The amount of residual lignin and hemicelluloses in softwood fibers was systematically varied by SO2-ethanol-water fractionation for integrated biorefinery with nanomaterial and biofuel production.
24686371	7	23	theme	complete	965:972	arg1	hydrolysis					979:988	Near complete LCNF hydrolysis	960:988	Near complete LCNF hydrolysis	960:988	Near complete LCNF hydrolysis was achieved, even at high lignin and hemicellulose content.
24686371	1	24	theme	softwood	210:217	arg1	fibers					219:224	softwood fibers	210:224	softwood fibers	210:224	The amount of residual lignin and hemicelluloses in softwood fibers was systematically varied by SO2-ethanol-water fractionation for integrated biorefinery with nanomaterial and biofuel production.
24686371	1	25	theme	biofuel	336:342	arg1	production					344:353	biofuel production	336:353	biofuel production	336:353	The amount of residual lignin and hemicelluloses in softwood fibers was systematically varied by SO2-ethanol-water fractionation for integrated biorefinery with nanomaterial and biofuel production.
24686371	8	26	theme	area	1164:1167	arg1	benefits					1139:1146	the benefits	1135:1146	the benefits of high surface area in LCNF	1135:1175	Sugar yields for LCNF were higher than those for precursor SEW fibers, highlighting the benefits of high surface area in LCNF.
24686371	6	27	theme	Enzyme	880:885	arg1	inhibition					887:896	Enzyme inhibition	880:896	Enzyme inhibition	880:896	Enzyme inhibition resulted from non-productive binding of proteins onto lignin.
24686371	0	28	theme	lignin	20:25	arg1	Effects					0:6	Effects	0:6	Effects of residual lignin and heteropolysaccharides on the bioconversion of softwood	0:84	Effects of residual lignin and heteropolysaccharides on the bioconversion of softwood lignocellulose nanofibrils obtained by SO2-ethanol-water fractionation.
24686371	7	29	theme	hemicellulose	1028:1040	arg1	content					1042:1048	hemicellulose content	1028:1048	hemicellulose content	1028:1048	Near complete LCNF hydrolysis was achieved, even at high lignin and hemicellulose content.
24686371	0	30	theme	residual	11:18	arg1	lignin					20:25	residual lignin	11:25	residual lignin	11:25	Effects of residual lignin and heteropolysaccharides on the bioconversion of softwood lignocellulose nanofibrils obtained by SO2-ethanol-water fractionation.
24686371	5	31	theme	lignin	796:801	arg1	concentration					821:833	lignin and hemicellulose concentration	796:833	lignin and hemicellulose concentration	796:833	LCNF hydrolysis rate gradually decreased with lignin and hemicellulose concentration, both of which limited enzyme accessibility.
24686371	2	32	dep	demand	389:394	arg1	basis					363:367	basis	363:367	basis	363:367	On the basis of their low energy demand in mechanical processing, the fibers were deconstructed to lignocellulose nanofibrils (LCNF) and used as substrate for bioconversion.
24686371	2	32	dep	demand	389:394	arg1	the					359:361	the	359:361	the	359:361	On the basis of their low energy demand in mechanical processing, the fibers were deconstructed to lignocellulose nanofibrils (LCNF) and used as substrate for bioconversion.
24686371	0	33	theme	heteropolysaccharides	31:51	arg1	Effects					0:6	Effects	0:6	Effects of residual lignin and heteropolysaccharides on the bioconversion of softwood	0:84	Effects of residual lignin and heteropolysaccharides on the bioconversion of softwood lignocellulose nanofibrils obtained by SO2-ethanol-water fractionation.
24686371	4	34	theme	quartz	721:726	arg1	microbalance					736:747	a quartz crystal microbalance	719:747	a quartz crystal microbalance	719:747	LCNF digestibility was compared with the enzyme activity measured with a quartz crystal microbalance.
24686371	5	35	theme	hemicellulose	807:819	arg1	concentration					821:833	lignin and hemicellulose concentration	796:833	lignin and hemicellulose concentration	796:833	LCNF hydrolysis rate gradually decreased with lignin and hemicellulose concentration, both of which limited enzyme accessibility.
24686371	5	36	theme	hydrolysis	755:764	arg1	rate					766:769	LCNF hydrolysis rate	750:769	LCNF hydrolysis rate	750:769	LCNF hydrolysis rate gradually decreased with lignin and hemicellulose concentration, both of which limited enzyme accessibility.
24686371	8	37	from	benefits	1139:1146	arg1	LCNF					1172:1175	LCNF	1172:1175	LCNF	1172:1175	Sugar yields for LCNF were higher than those for precursor SEW fibers, highlighting the benefits of high surface area in LCNF.
24686371	8	38	theme	Sugar	1051:1055	arg1	yields					1057:1062	Sugar yields	1051:1062	Sugar yields for LCNF	1051:1071	Sugar yields for LCNF were higher than those for precursor SEW fibers, highlighting the benefits of high surface area in LCNF.
24686371	1	39	with	biorefinery	302:312	arg1	nanomaterial					319:330	nanomaterial	319:330	nanomaterial	319:330	The amount of residual lignin and hemicelluloses in softwood fibers was systematically varied by SO2-ethanol-water fractionation for integrated biorefinery with nanomaterial and biofuel production.
24686371	1	39	with	biorefinery	302:312	arg1	production					344:353	biofuel production	336:353	biofuel production	336:353	The amount of residual lignin and hemicelluloses in softwood fibers was systematically varied by SO2-ethanol-water fractionation for integrated biorefinery with nanomaterial and biofuel production.
24686371	3	40	theme	LCNF	544:547	arg1	composition					549:559	LCNF composition	544:559	LCNF composition	544:559	The effect of LCNF composition on saccharification via multicomponent enzymes was investigated at different loadings.
24686371	2	41	theme	energy	382:387	arg1	demand					389:394	their low energy demand	372:394	their low energy demand in mechanical processing	372:419	On the basis of their low energy demand in mechanical processing, the fibers were deconstructed to lignocellulose nanofibrils (LCNF) and used as substrate for bioconversion.
24686371	1	42	theme	SO2-ethanol-water	255:271	arg1	fractionation					273:285	SO2-ethanol-water fractionation	255:285	SO2-ethanol-water fractionation	255:285	The amount of residual lignin and hemicelluloses in softwood fibers was systematically varied by SO2-ethanol-water fractionation for integrated biorefinery with nanomaterial and biofuel production.
24686371	2	43	theme	low	378:380	arg1	demand					389:394	their low energy demand	372:394	their low energy demand in mechanical processing	372:419	On the basis of their low energy demand in mechanical processing, the fibers were deconstructed to lignocellulose nanofibrils (LCNF) and used as substrate for bioconversion.
24686371	6	44	theme	proteins	938:945	arg1	binding					927:933	non-productive binding	912:933	non-productive binding of proteins onto lignin	912:957	Enzyme inhibition resulted from non-productive binding of proteins onto lignin.
24686371	7	45	theme	high	1012:1015	arg1	lignin					1017:1022	high lignin	1012:1022	high lignin	1012:1022	Near complete LCNF hydrolysis was achieved, even at high lignin and hemicellulose content.
24686371	4	46	theme	enzyme	689:694	arg1	activity					696:703	the enzyme activity	685:703	the enzyme activity measured with a quartz crystal microbalance	685:747	LCNF digestibility was compared with the enzyme activity measured with a quartz crystal microbalance.
24686371	1	47	theme	residual	172:179	arg1	lignin					181:186	residual lignin	172:186	residual lignin	172:186	The amount of residual lignin and hemicelluloses in softwood fibers was systematically varied by SO2-ethanol-water fractionation for integrated biorefinery with nanomaterial and biofuel production.
24686371	1	48	theme	integrated	291:300	arg1	biorefinery					302:312	integrated biorefinery	291:312	integrated biorefinery with nanomaterial and biofuel production	291:353	The amount of residual lignin and hemicelluloses in softwood fibers was systematically varied by SO2-ethanol-water fractionation for integrated biorefinery with nanomaterial and biofuel production.
27532123	4	0	theme	dismutase	959:967	arg1	activities					934:943	the activities	930:943	the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and total antioxidant capacity (T-AOC) in mice	930:1054	The in vivo anti-ageing evaluation showed that MSPS significantly reduced the malonaldehyde (MDA) contents and total cholesterol (CHOL) levels, and remarkably improved the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and total antioxidant capacity (T-AOC) in mice in response to D-galactose-induced ageing.
27532123	5	1	from	ratio	1342:1346	arg1	glucose					1309:1315	glucose	1309:1315	glucose	1309:1315	Furthermore, the characteristic analysis of MSPS indicated a selenium content of 1.76 ± 0.10 mg/g at a concentration of 6 μg/mL in liquid media and a monosaccharide composition of rhamnose, arabinose, mannose, glucose and galactose at a molar ratio of 29:3:1:18.8:2.7.
27532123	5	1	from	ratio	1342:1346	arg1	mannose					1300:1306	mannose	1300:1306	mannose	1300:1306	Furthermore, the characteristic analysis of MSPS indicated a selenium content of 1.76 ± 0.10 mg/g at a concentration of 6 μg/mL in liquid media and a monosaccharide composition of rhamnose, arabinose, mannose, glucose and galactose at a molar ratio of 29:3:1:18.8:2.7.
27532123	5	1	from	ratio	1342:1346	arg1	rhamnose					1279:1286	rhamnose	1279:1286	rhamnose	1279:1286	Furthermore, the characteristic analysis of MSPS indicated a selenium content of 1.76 ± 0.10 mg/g at a concentration of 6 μg/mL in liquid media and a monosaccharide composition of rhamnose, arabinose, mannose, glucose and galactose at a molar ratio of 29:3:1:18.8:2.7.
27532123	5	1	from	ratio	1342:1346	arg1	galactose					1321:1329	galactose	1321:1329	galactose	1321:1329	Furthermore, the characteristic analysis of MSPS indicated a selenium content of 1.76 ± 0.10 mg/g at a concentration of 6 μg/mL in liquid media and a monosaccharide composition of rhamnose, arabinose, mannose, glucose and galactose at a molar ratio of 29:3:1:18.8:2.7.
27532123	2	2	theme	precipitation	533:545	arg1	temperature					547:557	a precipitation temperature	531:557	a precipitation temperature of 12°C	531:565	The Box-Behnken experimental design (BBD) was evaluated, which showed that the optimum conditions included an extraction temperature of 94.99°C, a pH of 9 and a precipitation temperature of 12°C, and the predicted yield was 11.036 ± 0.31%.
27532123	1	3	theme	anti-ageing	348:358	arg1	activities					360:369	their in vitro antioxidant and in vivo anti-ageing activities	309:369	their in vitro antioxidant and in vivo anti-ageing activities	309:369	The aim of the present study was to optimize the purification of mycelia selenium polysaccharides (MSPS) from Agrocybe cylindracea SL-02 and characterize their in vitro antioxidant and in vivo anti-ageing activities.
27532123	2	4	theme	±	603:603	arg1	yield					586:590	the predicted yield	572:590	the predicted yield	572:590	The Box-Behnken experimental design (BBD) was evaluated, which showed that the optimum conditions included an extraction temperature of 94.99°C, a pH of 9 and a precipitation temperature of 12°C, and the predicted yield was 11.036 ± 0.31%.
27532123	2	4	theme	±	603:603	arg1	%					609:609	11.036 ± 0.31%	596:609	11.036 ± 0.31%	596:609	The Box-Behnken experimental design (BBD) was evaluated, which showed that the optimum conditions included an extraction temperature of 94.99°C, a pH of 9 and a precipitation temperature of 12°C, and the predicted yield was 11.036 ± 0.31%.
27532123	1	5	theme	present	170:176	arg1	study					178:182	the present study	166:182	the present study	166:182	The aim of the present study was to optimize the purification of mycelia selenium polysaccharides (MSPS) from Agrocybe cylindracea SL-02 and characterize their in vitro antioxidant and in vivo anti-ageing activities.
27532123	4	6	theme	total	1013:1017	arg1	capacity					1031:1038	total antioxidant capacity	1013:1038	total antioxidant capacity (T-AOC)	1013:1046	The in vivo anti-ageing evaluation showed that MSPS significantly reduced the malonaldehyde (MDA) contents and total cholesterol (CHOL) levels, and remarkably improved the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and total antioxidant capacity (T-AOC) in mice in response to D-galactose-induced ageing.
27532123	4	6	theme	total	1013:1017	arg1	T-AOC					1041:1045	T-AOC	1041:1045	T-AOC	1041:1045	The in vivo anti-ageing evaluation showed that MSPS significantly reduced the malonaldehyde (MDA) contents and total cholesterol (CHOL) levels, and remarkably improved the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and total antioxidant capacity (T-AOC) in mice in response to D-galactose-induced ageing.
27532123	3	7	theme	in	616:617	arg1	assay					637:641	The in vitro antioxidant assay	612:641	The in vitro antioxidant assay	612:641	The in vitro antioxidant assay demonstrated that MSPS had potential effects on scavenging and enhanced the reducing power of reactive oxygen species.
27532123	2	8	theme	9	525:525	arg1	pH					519:520	a pH	517:520	a pH of 9	517:525	The Box-Behnken experimental design (BBD) was evaluated, which showed that the optimum conditions included an extraction temperature of 94.99°C, a pH of 9 and a precipitation temperature of 12°C, and the predicted yield was 11.036 ± 0.31%.
27532123	2	8	theme	9	525:525	arg1	temperature					493:503	an extraction temperature	479:503	an extraction temperature of 94.99°C	479:514	The Box-Behnken experimental design (BBD) was evaluated, which showed that the optimum conditions included an extraction temperature of 94.99°C, a pH of 9 and a precipitation temperature of 12°C, and the predicted yield was 11.036 ± 0.31%.
27532123	2	8	theme	9	525:525	arg1	temperature					547:557	a precipitation temperature	531:557	a precipitation temperature of 12°C	531:565	The Box-Behnken experimental design (BBD) was evaluated, which showed that the optimum conditions included an extraction temperature of 94.99°C, a pH of 9 and a precipitation temperature of 12°C, and the predicted yield was 11.036 ± 0.31%.
27532123	0	9	from	Optimization	0:11	arg1	cylindracea					73:83	Agrocybe cylindracea SL-02	64:89	Agrocybe cylindracea SL-02	64:89	Optimization of Mycelia Selenium Polysaccharide Extraction from Agrocybe cylindracea SL-02 and Assessment of their Antioxidant and Anti-Ageing Activities.
27532123	2	10	theme	predicted	576:584	arg1	yield					586:590	the predicted yield	572:590	the predicted yield	572:590	The Box-Behnken experimental design (BBD) was evaluated, which showed that the optimum conditions included an extraction temperature of 94.99°C, a pH of 9 and a precipitation temperature of 12°C, and the predicted yield was 11.036 ± 0.31%.
27532123	2	10	theme	predicted	576:584	arg1	%					609:609	11.036 ± 0.31%	596:609	11.036 ± 0.31%	596:609	The Box-Behnken experimental design (BBD) was evaluated, which showed that the optimum conditions included an extraction temperature of 94.99°C, a pH of 9 and a precipitation temperature of 12°C, and the predicted yield was 11.036 ± 0.31%.
27532123	5	11	theme	29:3:1:18.8:2.7	1351:1365	arg1	ratio					1342:1346	a molar ratio	1334:1346	a molar ratio of 29:3:1:18.8:2.7	1334:1365	Furthermore, the characteristic analysis of MSPS indicated a selenium content of 1.76 ± 0.10 mg/g at a concentration of 6 μg/mL in liquid media and a monosaccharide composition of rhamnose, arabinose, mannose, glucose and galactose at a molar ratio of 29:3:1:18.8:2.7.
27532123	6	12	theme	ageing	1475:1480	arg1	progress					1482:1489	the ageing progress	1471:1489	the ageing progress induced by toxic chemicals	1471:1516	These results suggest that MSPS might be suitable for functional foods and natural drugs on preventing the ageing progress induced by toxic chemicals.
27532123	1	13	theme	Agrocybe	265:272	arg1	SL-02					286:290	Agrocybe cylindracea SL-02	265:290	Agrocybe cylindracea SL-02	265:290	The aim of the present study was to optimize the purification of mycelia selenium polysaccharides (MSPS) from Agrocybe cylindracea SL-02 and characterize their in vitro antioxidant and in vivo anti-ageing activities.
27532123	4	14	theme	peroxidase	988:997	arg1	activities					934:943	the activities	930:943	the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and total antioxidant capacity (T-AOC) in mice	930:1054	The in vivo anti-ageing evaluation showed that MSPS significantly reduced the malonaldehyde (MDA) contents and total cholesterol (CHOL) levels, and remarkably improved the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and total antioxidant capacity (T-AOC) in mice in response to D-galactose-induced ageing.
27532123	5	15	from	concentration	1202:1214	arg1	composition					1264:1274	a monosaccharide composition	1247:1274	a monosaccharide composition of rhamnose, arabinose, mannose, glucose and galactose at a molar ratio of 29:3:1:18.8:2.7	1247:1365	Furthermore, the characteristic analysis of MSPS indicated a selenium content of 1.76 ± 0.10 mg/g at a concentration of 6 μg/mL in liquid media and a monosaccharide composition of rhamnose, arabinose, mannose, glucose and galactose at a molar ratio of 29:3:1:18.8:2.7.
27532123	5	15	from	concentration	1202:1214	arg1	mg/g					1192:1195	1.76 ± 0.10 mg/g	1180:1195	1.76 ± 0.10 mg/g at a concentration of 6 μg/mL in liquid media	1180:1241	Furthermore, the characteristic analysis of MSPS indicated a selenium content of 1.76 ± 0.10 mg/g at a concentration of 6 μg/mL in liquid media and a monosaccharide composition of rhamnose, arabinose, mannose, glucose and galactose at a molar ratio of 29:3:1:18.8:2.7.
27532123	5	15	from	concentration	1202:1214	arg1	content					1169:1175	a selenium content	1158:1175	a selenium content of 1.76 ± 0.10 mg/g at a concentration of 6 μg/mL in liquid media	1158:1241	Furthermore, the characteristic analysis of MSPS indicated a selenium content of 1.76 ± 0.10 mg/g at a concentration of 6 μg/mL in liquid media and a monosaccharide composition of rhamnose, arabinose, mannose, glucose and galactose at a molar ratio of 29:3:1:18.8:2.7.
27532123	5	15	from	concentration	1202:1214	arg1	media					1237:1241	liquid media	1230:1241	liquid media	1230:1241	Furthermore, the characteristic analysis of MSPS indicated a selenium content of 1.76 ± 0.10 mg/g at a concentration of 6 μg/mL in liquid media and a monosaccharide composition of rhamnose, arabinose, mannose, glucose and galactose at a molar ratio of 29:3:1:18.8:2.7.
27532123	5	16	theme	monosaccharide	1249:1262	arg1	composition					1264:1274	a monosaccharide composition	1247:1274	a monosaccharide composition of rhamnose, arabinose, mannose, glucose and galactose at a molar ratio of 29:3:1:18.8:2.7	1247:1365	Furthermore, the characteristic analysis of MSPS indicated a selenium content of 1.76 ± 0.10 mg/g at a concentration of 6 μg/mL in liquid media and a monosaccharide composition of rhamnose, arabinose, mannose, glucose and galactose at a molar ratio of 29:3:1:18.8:2.7.
27532123	1	17	theme	cylindracea	274:284	arg1	SL-02					286:290	Agrocybe cylindracea SL-02	265:290	Agrocybe cylindracea SL-02	265:290	The aim of the present study was to optimize the purification of mycelia selenium polysaccharides (MSPS) from Agrocybe cylindracea SL-02 and characterize their in vitro antioxidant and in vivo anti-ageing activities.
27532123	1	18	theme	study	178:182	arg1	aim					159:161	The aim	155:161	The aim of the present study	155:182	The aim of the present study was to optimize the purification of mycelia selenium polysaccharides (MSPS) from Agrocybe cylindracea SL-02 and characterize their in vitro antioxidant and in vivo anti-ageing activities.
27532123	0	19	theme	Antioxidant	115:125	arg1	Activities					143:152	their Antioxidant and Anti-Ageing Activities	109:152	their Antioxidant and Anti-Ageing Activities	109:152	Optimization of Mycelia Selenium Polysaccharide Extraction from Agrocybe cylindracea SL-02 and Assessment of their Antioxidant and Anti-Ageing Activities.
27532123	1	20	dep	in	315:316	arg1	vitro					318:322	vitro	318:322	vitro	318:322	The aim of the present study was to optimize the purification of mycelia selenium polysaccharides (MSPS) from Agrocybe cylindracea SL-02 and characterize their in vitro antioxidant and in vivo anti-ageing activities.
27532123	1	20	dep	in	315:316	arg1	antioxidant					324:334	antioxidant	324:334	antioxidant	324:334	The aim of the present study was to optimize the purification of mycelia selenium polysaccharides (MSPS) from Agrocybe cylindracea SL-02 and characterize their in vitro antioxidant and in vivo anti-ageing activities.
27532123	2	21	theme	extraction	482:491	arg1	temperature					493:503	an extraction temperature	479:503	an extraction temperature of 94.99°C	479:514	The Box-Behnken experimental design (BBD) was evaluated, which showed that the optimum conditions included an extraction temperature of 94.99°C, a pH of 9 and a precipitation temperature of 12°C, and the predicted yield was 11.036 ± 0.31%.
27532123	2	22	theme	optimum	451:457	arg1	conditions					459:468	the optimum conditions	447:468	the optimum conditions	447:468	The Box-Behnken experimental design (BBD) was evaluated, which showed that the optimum conditions included an extraction temperature of 94.99°C, a pH of 9 and a precipitation temperature of 12°C, and the predicted yield was 11.036 ± 0.31%.
27532123	4	23	theme	superoxide	948:957	arg1	SOD					970:972	SOD	970:972	SOD	970:972	The in vivo anti-ageing evaluation showed that MSPS significantly reduced the malonaldehyde (MDA) contents and total cholesterol (CHOL) levels, and remarkably improved the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and total antioxidant capacity (T-AOC) in mice in response to D-galactose-induced ageing.
27532123	4	23	theme	superoxide	948:957	arg1	dismutase					959:967	superoxide dismutase	948:967	superoxide dismutase (SOD)	948:973	The in vivo anti-ageing evaluation showed that MSPS significantly reduced the malonaldehyde (MDA) contents and total cholesterol (CHOL) levels, and remarkably improved the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and total antioxidant capacity (T-AOC) in mice in response to D-galactose-induced ageing.
27532123	1	24	from	SL-02	286:290	arg1	purification					204:215	the purification	200:215	the purification of mycelia selenium polysaccharides (MSPS) from Agrocybe cylindracea SL-02	200:290	The aim of the present study was to optimize the purification of mycelia selenium polysaccharides (MSPS) from Agrocybe cylindracea SL-02 and characterize their in vitro antioxidant and in vivo anti-ageing activities.
27532123	5	25	theme	rhamnose	1279:1286	arg1	composition					1264:1274	a monosaccharide composition	1247:1274	a monosaccharide composition of rhamnose, arabinose, mannose, glucose and galactose at a molar ratio of 29:3:1:18.8:2.7	1247:1365	Furthermore, the characteristic analysis of MSPS indicated a selenium content of 1.76 ± 0.10 mg/g at a concentration of 6 μg/mL in liquid media and a monosaccharide composition of rhamnose, arabinose, mannose, glucose and galactose at a molar ratio of 29:3:1:18.8:2.7.
27532123	5	25	theme	rhamnose	1279:1286	arg1	content					1169:1175	a selenium content	1158:1175	a selenium content of 1.76 ± 0.10 mg/g at a concentration of 6 μg/mL in liquid media	1158:1241	Furthermore, the characteristic analysis of MSPS indicated a selenium content of 1.76 ± 0.10 mg/g at a concentration of 6 μg/mL in liquid media and a monosaccharide composition of rhamnose, arabinose, mannose, glucose and galactose at a molar ratio of 29:3:1:18.8:2.7.
27532123	4	26	theme	D-galactose-induced	1071:1089	arg1	ageing					1091:1096	D-galactose-induced ageing	1071:1096	D-galactose-induced ageing	1071:1096	The in vivo anti-ageing evaluation showed that MSPS significantly reduced the malonaldehyde (MDA) contents and total cholesterol (CHOL) levels, and remarkably improved the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and total antioxidant capacity (T-AOC) in mice in response to D-galactose-induced ageing.
27532123	4	27	dep	in	766:767	arg1	vivo					769:772	vivo	769:772	vivo	769:772	The in vivo anti-ageing evaluation showed that MSPS significantly reduced the malonaldehyde (MDA) contents and total cholesterol (CHOL) levels, and remarkably improved the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and total antioxidant capacity (T-AOC) in mice in response to D-galactose-induced ageing.
27532123	4	28	theme	glutathione	976:986	arg1	peroxidase					988:997	glutathione peroxidase	976:997	glutathione peroxidase (GSH-Px)	976:1006	The in vivo anti-ageing evaluation showed that MSPS significantly reduced the malonaldehyde (MDA) contents and total cholesterol (CHOL) levels, and remarkably improved the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and total antioxidant capacity (T-AOC) in mice in response to D-galactose-induced ageing.
27532123	4	28	theme	glutathione	976:986	arg1	GSH-Px					1000:1005	GSH-Px	1000:1005	GSH-Px	1000:1005	The in vivo anti-ageing evaluation showed that MSPS significantly reduced the malonaldehyde (MDA) contents and total cholesterol (CHOL) levels, and remarkably improved the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and total antioxidant capacity (T-AOC) in mice in response to D-galactose-induced ageing.
27532123	5	29	theme	characteristic	1116:1129	arg1	analysis					1131:1138	the characteristic analysis	1112:1138	the characteristic analysis of MSPS	1112:1146	Furthermore, the characteristic analysis of MSPS indicated a selenium content of 1.76 ± 0.10 mg/g at a concentration of 6 μg/mL in liquid media and a monosaccharide composition of rhamnose, arabinose, mannose, glucose and galactose at a molar ratio of 29:3:1:18.8:2.7.
27532123	2	30	theme	11.036	596:601	arg1	yield					586:590	the predicted yield	572:590	the predicted yield	572:590	The Box-Behnken experimental design (BBD) was evaluated, which showed that the optimum conditions included an extraction temperature of 94.99°C, a pH of 9 and a precipitation temperature of 12°C, and the predicted yield was 11.036 ± 0.31%.
27532123	2	30	theme	11.036	596:601	arg1	%					609:609	11.036 ± 0.31%	596:609	11.036 ± 0.31%	596:609	The Box-Behnken experimental design (BBD) was evaluated, which showed that the optimum conditions included an extraction temperature of 94.99°C, a pH of 9 and a precipitation temperature of 12°C, and the predicted yield was 11.036 ± 0.31%.
27532123	5	31	theme	molar	1336:1340	arg1	ratio					1342:1346	a molar ratio	1334:1346	a molar ratio of 29:3:1:18.8:2.7	1334:1365	Furthermore, the characteristic analysis of MSPS indicated a selenium content of 1.76 ± 0.10 mg/g at a concentration of 6 μg/mL in liquid media and a monosaccharide composition of rhamnose, arabinose, mannose, glucose and galactose at a molar ratio of 29:3:1:18.8:2.7.
27532123	5	32	theme	liquid	1230:1235	arg1	media					1237:1241	liquid media	1230:1241	liquid media	1230:1241	Furthermore, the characteristic analysis of MSPS indicated a selenium content of 1.76 ± 0.10 mg/g at a concentration of 6 μg/mL in liquid media and a monosaccharide composition of rhamnose, arabinose, mannose, glucose and galactose at a molar ratio of 29:3:1:18.8:2.7.
27532123	0	33	theme	Selenium	24:31	arg1	Extraction					48:57	Mycelia Selenium Polysaccharide Extraction	16:57	Mycelia Selenium Polysaccharide Extraction from Agrocybe cylindracea SL-02	16:89	Optimization of Mycelia Selenium Polysaccharide Extraction from Agrocybe cylindracea SL-02 and Assessment of their Antioxidant and Anti-Ageing Activities.
27532123	0	34	theme	Activities	143:152	arg1	Optimization					0:11	Optimization	0:11	Optimization of Mycelia Selenium Polysaccharide Extraction from Agrocybe cylindracea SL-02	0:89	Optimization of Mycelia Selenium Polysaccharide Extraction from Agrocybe cylindracea SL-02 and Assessment of their Antioxidant and Anti-Ageing Activities.
27532123	0	34	theme	Activities	143:152	arg1	Assessment					95:104	Assessment	95:104	Assessment of their Antioxidant and Anti-Ageing Activities	95:152	Optimization of Mycelia Selenium Polysaccharide Extraction from Agrocybe cylindracea SL-02 and Assessment of their Antioxidant and Anti-Ageing Activities.
27532123	0	35	theme	Mycelia	16:22	arg1	Extraction					48:57	Mycelia Selenium Polysaccharide Extraction	16:57	Mycelia Selenium Polysaccharide Extraction from Agrocybe cylindracea SL-02	16:89	Optimization of Mycelia Selenium Polysaccharide Extraction from Agrocybe cylindracea SL-02 and Assessment of their Antioxidant and Anti-Ageing Activities.
27532123	1	36	dep	in	340:341	arg1	vivo					343:346	vivo	343:346	vivo	343:346	The aim of the present study was to optimize the purification of mycelia selenium polysaccharides (MSPS) from Agrocybe cylindracea SL-02 and characterize their in vitro antioxidant and in vivo anti-ageing activities.
27532123	0	37	theme	Anti-Ageing	131:141	arg1	Activities					143:152	their Antioxidant and Anti-Ageing Activities	109:152	their Antioxidant and Anti-Ageing Activities	109:152	Optimization of Mycelia Selenium Polysaccharide Extraction from Agrocybe cylindracea SL-02 and Assessment of their Antioxidant and Anti-Ageing Activities.
27532123	0	38	from	Assessment	95:104	arg1	cylindracea					73:83	Agrocybe cylindracea SL-02	64:89	Agrocybe cylindracea SL-02	64:89	Optimization of Mycelia Selenium Polysaccharide Extraction from Agrocybe cylindracea SL-02 and Assessment of their Antioxidant and Anti-Ageing Activities.
27532123	4	39	theme	malonaldehyde	840:852	arg1	contents					860:867	the malonaldehyde (MDA) contents	836:867	the malonaldehyde (MDA) contents	836:867	The in vivo anti-ageing evaluation showed that MSPS significantly reduced the malonaldehyde (MDA) contents and total cholesterol (CHOL) levels, and remarkably improved the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and total antioxidant capacity (T-AOC) in mice in response to D-galactose-induced ageing.
27532123	3	40	contain	had	666:668	arg1	MSPS					661:664	MSPS	661:664	MSPS	661:664	The in vitro antioxidant assay demonstrated that MSPS had potential effects on scavenging and enhanced the reducing power of reactive oxygen species.
27532123	3	40	contain	had	666:668	arg2	effects					680:686	potential effects	670:686	potential effects	670:686	The in vitro antioxidant assay demonstrated that MSPS had potential effects on scavenging and enhanced the reducing power of reactive oxygen species.
27532123	0	41	theme	Extraction	48:57	arg1	Optimization					0:11	Optimization	0:11	Optimization of Mycelia Selenium Polysaccharide Extraction from Agrocybe cylindracea SL-02	0:89	Optimization of Mycelia Selenium Polysaccharide Extraction from Agrocybe cylindracea SL-02 and Assessment of their Antioxidant and Anti-Ageing Activities.
27532123	0	41	theme	Extraction	48:57	arg1	Assessment					95:104	Assessment	95:104	Assessment of their Antioxidant and Anti-Ageing Activities	95:152	Optimization of Mycelia Selenium Polysaccharide Extraction from Agrocybe cylindracea SL-02 and Assessment of their Antioxidant and Anti-Ageing Activities.
27532123	3	42	theme	reducing	719:726	arg1	power					728:732	the reducing power	715:732	the reducing power of reactive oxygen species	715:759	The in vitro antioxidant assay demonstrated that MSPS had potential effects on scavenging and enhanced the reducing power of reactive oxygen species.
27532123	6	43	theme	functional	1422:1431	arg1	foods					1433:1437	functional foods	1422:1437	functional foods	1422:1437	These results suggest that MSPS might be suitable for functional foods and natural drugs on preventing the ageing progress induced by toxic chemicals.
27532123	0	44	theme	Polysaccharide	33:46	arg1	Extraction					48:57	Mycelia Selenium Polysaccharide Extraction	16:57	Mycelia Selenium Polysaccharide Extraction from Agrocybe cylindracea SL-02	16:89	Optimization of Mycelia Selenium Polysaccharide Extraction from Agrocybe cylindracea SL-02 and Assessment of their Antioxidant and Anti-Ageing Activities.
27532123	1	45	theme	in	315:316	arg1	activities					360:369	their in vitro antioxidant and in vivo anti-ageing activities	309:369	their in vitro antioxidant and in vivo anti-ageing activities	309:369	The aim of the present study was to optimize the purification of mycelia selenium polysaccharides (MSPS) from Agrocybe cylindracea SL-02 and characterize their in vitro antioxidant and in vivo anti-ageing activities.
27532123	5	46	theme	±	1185:1185	arg1	mg/g					1192:1195	1.76 ± 0.10 mg/g	1180:1195	1.76 ± 0.10 mg/g at a concentration of 6 μg/mL in liquid media	1180:1241	Furthermore, the characteristic analysis of MSPS indicated a selenium content of 1.76 ± 0.10 mg/g at a concentration of 6 μg/mL in liquid media and a monosaccharide composition of rhamnose, arabinose, mannose, glucose and galactose at a molar ratio of 29:3:1:18.8:2.7.
27532123	5	47	theme	MSPS	1143:1146	arg1	analysis					1131:1138	the characteristic analysis	1112:1138	the characteristic analysis of MSPS	1112:1146	Furthermore, the characteristic analysis of MSPS indicated a selenium content of 1.76 ± 0.10 mg/g at a concentration of 6 μg/mL in liquid media and a monosaccharide composition of rhamnose, arabinose, mannose, glucose and galactose at a molar ratio of 29:3:1:18.8:2.7.
27532123	1	48	theme	mycelia	220:226	arg1	MSPS					254:257	MSPS	254:257	MSPS	254:257	The aim of the present study was to optimize the purification of mycelia selenium polysaccharides (MSPS) from Agrocybe cylindracea SL-02 and characterize their in vitro antioxidant and in vivo anti-ageing activities.
27532123	1	48	theme	mycelia	220:226	arg1	polysaccharides					237:251	mycelia selenium polysaccharides	220:251	mycelia selenium polysaccharides (MSPS)	220:258	The aim of the present study was to optimize the purification of mycelia selenium polysaccharides (MSPS) from Agrocybe cylindracea SL-02 and characterize their in vitro antioxidant and in vivo anti-ageing activities.
27532123	0	49	theme	Agrocybe	64:71	arg1	cylindracea					73:83	Agrocybe cylindracea SL-02	64:89	Agrocybe cylindracea SL-02	64:89	Optimization of Mycelia Selenium Polysaccharide Extraction from Agrocybe cylindracea SL-02 and Assessment of their Antioxidant and Anti-Ageing Activities.
27532123	3	50	theme	species	753:759	arg1	power					728:732	the reducing power	715:732	the reducing power of reactive oxygen species	715:759	The in vitro antioxidant assay demonstrated that MSPS had potential effects on scavenging and enhanced the reducing power of reactive oxygen species.
27532123	4	51	theme	total	873:877	arg1	cholesterol					879:889	total cholesterol	873:889	total cholesterol (CHOL)	873:896	The in vivo anti-ageing evaluation showed that MSPS significantly reduced the malonaldehyde (MDA) contents and total cholesterol (CHOL) levels, and remarkably improved the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and total antioxidant capacity (T-AOC) in mice in response to D-galactose-induced ageing.
27532123	4	51	theme	total	873:877	arg1	CHOL					892:895	CHOL	892:895	CHOL	892:895	The in vivo anti-ageing evaluation showed that MSPS significantly reduced the malonaldehyde (MDA) contents and total cholesterol (CHOL) levels, and remarkably improved the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and total antioxidant capacity (T-AOC) in mice in response to D-galactose-induced ageing.
27532123	0	52	from	cylindracea	73:83	arg1	Extraction					48:57	Mycelia Selenium Polysaccharide Extraction	16:57	Mycelia Selenium Polysaccharide Extraction from Agrocybe cylindracea SL-02	16:89	Optimization of Mycelia Selenium Polysaccharide Extraction from Agrocybe cylindracea SL-02 and Assessment of their Antioxidant and Anti-Ageing Activities.
27532123	0	52	from	cylindracea	73:83	arg1	Optimization					0:11	Optimization	0:11	Optimization of Mycelia Selenium Polysaccharide Extraction from Agrocybe cylindracea SL-02	0:89	Optimization of Mycelia Selenium Polysaccharide Extraction from Agrocybe cylindracea SL-02 and Assessment of their Antioxidant and Anti-Ageing Activities.
27532123	0	52	from	cylindracea	73:83	arg1	Assessment					95:104	Assessment	95:104	Assessment of their Antioxidant and Anti-Ageing Activities	95:152	Optimization of Mycelia Selenium Polysaccharide Extraction from Agrocybe cylindracea SL-02 and Assessment of their Antioxidant and Anti-Ageing Activities.
27532123	3	53	theme	reactive	737:744	arg1	species					753:759	reactive oxygen species	737:759	reactive oxygen species	737:759	The in vitro antioxidant assay demonstrated that MSPS had potential effects on scavenging and enhanced the reducing power of reactive oxygen species.
27532123	3	54	theme	potential	670:678	arg1	effects					680:686	potential effects	670:686	potential effects	670:686	The in vitro antioxidant assay demonstrated that MSPS had potential effects on scavenging and enhanced the reducing power of reactive oxygen species.
27532123	5	55	from	content	1169:1175	arg1	concentration					1202:1214	a concentration	1200:1214	a concentration of 6 μg/mL in liquid media	1200:1241	Furthermore, the characteristic analysis of MSPS indicated a selenium content of 1.76 ± 0.10 mg/g at a concentration of 6 μg/mL in liquid media and a monosaccharide composition of rhamnose, arabinose, mannose, glucose and galactose at a molar ratio of 29:3:1:18.8:2.7.
27532123	4	56	theme	contents	860:867	arg1	levels					898:903	the malonaldehyde (MDA) contents and total cholesterol (CHOL) levels	836:903	the malonaldehyde (MDA) contents and total cholesterol (CHOL) levels	836:903	The in vivo anti-ageing evaluation showed that MSPS significantly reduced the malonaldehyde (MDA) contents and total cholesterol (CHOL) levels, and remarkably improved the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and total antioxidant capacity (T-AOC) in mice in response to D-galactose-induced ageing.
27532123	4	57	theme	capacity	1031:1038	arg1	activities					934:943	the activities	930:943	the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and total antioxidant capacity (T-AOC) in mice	930:1054	The in vivo anti-ageing evaluation showed that MSPS significantly reduced the malonaldehyde (MDA) contents and total cholesterol (CHOL) levels, and remarkably improved the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and total antioxidant capacity (T-AOC) in mice in response to D-galactose-induced ageing.
27532123	3	58	theme	oxygen	746:751	arg1	species					753:759	reactive oxygen species	737:759	reactive oxygen species	737:759	The in vitro antioxidant assay demonstrated that MSPS had potential effects on scavenging and enhanced the reducing power of reactive oxygen species.
27532123	2	59	theme	12°C	562:565	arg1	pH					519:520	a pH	517:520	a pH of 9	517:525	The Box-Behnken experimental design (BBD) was evaluated, which showed that the optimum conditions included an extraction temperature of 94.99°C, a pH of 9 and a precipitation temperature of 12°C, and the predicted yield was 11.036 ± 0.31%.
27532123	2	59	theme	12°C	562:565	arg1	temperature					493:503	an extraction temperature	479:503	an extraction temperature of 94.99°C	479:514	The Box-Behnken experimental design (BBD) was evaluated, which showed that the optimum conditions included an extraction temperature of 94.99°C, a pH of 9 and a precipitation temperature of 12°C, and the predicted yield was 11.036 ± 0.31%.
27532123	2	59	theme	12°C	562:565	arg1	temperature					547:557	a precipitation temperature	531:557	a precipitation temperature of 12°C	531:565	The Box-Behnken experimental design (BBD) was evaluated, which showed that the optimum conditions included an extraction temperature of 94.99°C, a pH of 9 and a precipitation temperature of 12°C, and the predicted yield was 11.036 ± 0.31%.
27532123	4	60	theme	in	766:767	arg1	evaluation					786:795	The in vivo anti-ageing evaluation	762:795	The in vivo anti-ageing evaluation	762:795	The in vivo anti-ageing evaluation showed that MSPS significantly reduced the malonaldehyde (MDA) contents and total cholesterol (CHOL) levels, and remarkably improved the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and total antioxidant capacity (T-AOC) in mice in response to D-galactose-induced ageing.
27532123	6	61	theme	toxic	1502:1506	arg1	chemicals					1508:1516	toxic chemicals	1502:1516	toxic chemicals	1502:1516	These results suggest that MSPS might be suitable for functional foods and natural drugs on preventing the ageing progress induced by toxic chemicals.
27532123	4	62	theme	antioxidant	1019:1029	arg1	capacity					1031:1038	total antioxidant capacity	1013:1038	total antioxidant capacity (T-AOC)	1013:1046	The in vivo anti-ageing evaluation showed that MSPS significantly reduced the malonaldehyde (MDA) contents and total cholesterol (CHOL) levels, and remarkably improved the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and total antioxidant capacity (T-AOC) in mice in response to D-galactose-induced ageing.
27532123	4	62	theme	antioxidant	1019:1029	arg1	T-AOC					1041:1045	T-AOC	1041:1045	T-AOC	1041:1045	The in vivo anti-ageing evaluation showed that MSPS significantly reduced the malonaldehyde (MDA) contents and total cholesterol (CHOL) levels, and remarkably improved the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and total antioxidant capacity (T-AOC) in mice in response to D-galactose-induced ageing.
27532123	1	63	theme	selenium	228:235	arg1	MSPS					254:257	MSPS	254:257	MSPS	254:257	The aim of the present study was to optimize the purification of mycelia selenium polysaccharides (MSPS) from Agrocybe cylindracea SL-02 and characterize their in vitro antioxidant and in vivo anti-ageing activities.
27532123	1	63	theme	selenium	228:235	arg1	polysaccharides					237:251	mycelia selenium polysaccharides	220:251	mycelia selenium polysaccharides (MSPS)	220:258	The aim of the present study was to optimize the purification of mycelia selenium polysaccharides (MSPS) from Agrocybe cylindracea SL-02 and characterize their in vitro antioxidant and in vivo anti-ageing activities.
27532123	4	64	theme	cholesterol	879:889	arg1	levels					898:903	the malonaldehyde (MDA) contents and total cholesterol (CHOL) levels	836:903	the malonaldehyde (MDA) contents and total cholesterol (CHOL) levels	836:903	The in vivo anti-ageing evaluation showed that MSPS significantly reduced the malonaldehyde (MDA) contents and total cholesterol (CHOL) levels, and remarkably improved the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and total antioxidant capacity (T-AOC) in mice in response to D-galactose-induced ageing.
27532123	3	65	theme	antioxidant	625:635	arg1	assay					637:641	The in vitro antioxidant assay	612:641	The in vitro antioxidant assay	612:641	The in vitro antioxidant assay demonstrated that MSPS had potential effects on scavenging and enhanced the reducing power of reactive oxygen species.
27532123	5	66	theme	μg/mL	1221:1225	arg1	concentration					1202:1214	a concentration	1200:1214	a concentration of 6 μg/mL in liquid media	1200:1241	Furthermore, the characteristic analysis of MSPS indicated a selenium content of 1.76 ± 0.10 mg/g at a concentration of 6 μg/mL in liquid media and a monosaccharide composition of rhamnose, arabinose, mannose, glucose and galactose at a molar ratio of 29:3:1:18.8:2.7.
27532123	1	67	theme	polysaccharides	237:251	arg1	purification					204:215	the purification	200:215	the purification of mycelia selenium polysaccharides (MSPS) from Agrocybe cylindracea SL-02	200:290	The aim of the present study was to optimize the purification of mycelia selenium polysaccharides (MSPS) from Agrocybe cylindracea SL-02 and characterize their in vitro antioxidant and in vivo anti-ageing activities.
27532123	6	68	theme	natural	1443:1449	arg1	drugs					1451:1455	natural drugs	1443:1455	natural drugs	1443:1455	These results suggest that MSPS might be suitable for functional foods and natural drugs on preventing the ageing progress induced by toxic chemicals.
27532123	4	69	theme	anti-ageing	774:784	arg1	evaluation					786:795	The in vivo anti-ageing evaluation	762:795	The in vivo anti-ageing evaluation	762:795	The in vivo anti-ageing evaluation showed that MSPS significantly reduced the malonaldehyde (MDA) contents and total cholesterol (CHOL) levels, and remarkably improved the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and total antioxidant capacity (T-AOC) in mice in response to D-galactose-induced ageing.
27532123	2	70	theme	experimental	388:399	arg1	BBD					409:411	BBD	409:411	BBD	409:411	The Box-Behnken experimental design (BBD) was evaluated, which showed that the optimum conditions included an extraction temperature of 94.99°C, a pH of 9 and a precipitation temperature of 12°C, and the predicted yield was 11.036 ± 0.31%.
27532123	2	70	theme	experimental	388:399	arg1	design					401:406	The Box-Behnken experimental design	372:406	The Box-Behnken experimental design (BBD)	372:412	The Box-Behnken experimental design (BBD) was evaluated, which showed that the optimum conditions included an extraction temperature of 94.99°C, a pH of 9 and a precipitation temperature of 12°C, and the predicted yield was 11.036 ± 0.31%.
27532123	3	71	dep	in	616:617	arg1	vitro					619:623	vitro	619:623	vitro	619:623	The in vitro antioxidant assay demonstrated that MSPS had potential effects on scavenging and enhanced the reducing power of reactive oxygen species.
27532123	2	72	theme	94.99°C	508:514	arg1	pH					519:520	a pH	517:520	a pH of 9	517:525	The Box-Behnken experimental design (BBD) was evaluated, which showed that the optimum conditions included an extraction temperature of 94.99°C, a pH of 9 and a precipitation temperature of 12°C, and the predicted yield was 11.036 ± 0.31%.
27532123	2	72	theme	94.99°C	508:514	arg1	temperature					493:503	an extraction temperature	479:503	an extraction temperature of 94.99°C	479:514	The Box-Behnken experimental design (BBD) was evaluated, which showed that the optimum conditions included an extraction temperature of 94.99°C, a pH of 9 and a precipitation temperature of 12°C, and the predicted yield was 11.036 ± 0.31%.
27532123	2	72	theme	94.99°C	508:514	arg1	temperature					547:557	a precipitation temperature	531:557	a precipitation temperature of 12°C	531:565	The Box-Behnken experimental design (BBD) was evaluated, which showed that the optimum conditions included an extraction temperature of 94.99°C, a pH of 9 and a precipitation temperature of 12°C, and the predicted yield was 11.036 ± 0.31%.
27532123	1	73	theme	in	340:341	arg1	activities					360:369	their in vitro antioxidant and in vivo anti-ageing activities	309:369	their in vitro antioxidant and in vivo anti-ageing activities	309:369	The aim of the present study was to optimize the purification of mycelia selenium polysaccharides (MSPS) from Agrocybe cylindracea SL-02 and characterize their in vitro antioxidant and in vivo anti-ageing activities.
27532123	5	74	from	composition	1264:1274	arg1	concentration					1202:1214	a concentration	1200:1214	a concentration of 6 μg/mL in liquid media	1200:1241	Furthermore, the characteristic analysis of MSPS indicated a selenium content of 1.76 ± 0.10 mg/g at a concentration of 6 μg/mL in liquid media and a monosaccharide composition of rhamnose, arabinose, mannose, glucose and galactose at a molar ratio of 29:3:1:18.8:2.7.
27532123	5	75	theme	selenium	1160:1167	arg1	content					1169:1175	a selenium content	1158:1175	a selenium content of 1.76 ± 0.10 mg/g at a concentration of 6 μg/mL in liquid media	1158:1241	Furthermore, the characteristic analysis of MSPS indicated a selenium content of 1.76 ± 0.10 mg/g at a concentration of 6 μg/mL in liquid media and a monosaccharide composition of rhamnose, arabinose, mannose, glucose and galactose at a molar ratio of 29:3:1:18.8:2.7.
27532123	2	76	theme	Box-Behnken	376:386	arg1	BBD					409:411	BBD	409:411	BBD	409:411	The Box-Behnken experimental design (BBD) was evaluated, which showed that the optimum conditions included an extraction temperature of 94.99°C, a pH of 9 and a precipitation temperature of 12°C, and the predicted yield was 11.036 ± 0.31%.
27532123	2	76	theme	Box-Behnken	376:386	arg1	design					401:406	The Box-Behnken experimental design	372:406	The Box-Behnken experimental design (BBD)	372:412	The Box-Behnken experimental design (BBD) was evaluated, which showed that the optimum conditions included an extraction temperature of 94.99°C, a pH of 9 and a precipitation temperature of 12°C, and the predicted yield was 11.036 ± 0.31%.
27532123	5	77	theme	mg/g	1192:1195	arg1	composition					1264:1274	a monosaccharide composition	1247:1274	a monosaccharide composition of rhamnose, arabinose, mannose, glucose and galactose at a molar ratio of 29:3:1:18.8:2.7	1247:1365	Furthermore, the characteristic analysis of MSPS indicated a selenium content of 1.76 ± 0.10 mg/g at a concentration of 6 μg/mL in liquid media and a monosaccharide composition of rhamnose, arabinose, mannose, glucose and galactose at a molar ratio of 29:3:1:18.8:2.7.
27532123	5	77	theme	mg/g	1192:1195	arg1	content					1169:1175	a selenium content	1158:1175	a selenium content of 1.76 ± 0.10 mg/g at a concentration of 6 μg/mL in liquid media	1158:1241	Furthermore, the characteristic analysis of MSPS indicated a selenium content of 1.76 ± 0.10 mg/g at a concentration of 6 μg/mL in liquid media and a monosaccharide composition of rhamnose, arabinose, mannose, glucose and galactose at a molar ratio of 29:3:1:18.8:2.7.
27532123	4	78	from	activities	934:943	arg1	mice					1051:1054	mice	1051:1054	mice	1051:1054	The in vivo anti-ageing evaluation showed that MSPS significantly reduced the malonaldehyde (MDA) contents and total cholesterol (CHOL) levels, and remarkably improved the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and total antioxidant capacity (T-AOC) in mice in response to D-galactose-induced ageing.
25439906	0	0	theme	structural	62:71	arg1	variations					73:82	the structural variations	58:82	the structural variations in thermoplastic starch	58:106	Studies on the effect of storage time and plasticizers on the structural variations in thermoplastic starch.
25439906	1	1	theme	shear	239:243	arg1	extrusion					245:253	high shear extrusion	234:253	high shear extrusion process to prepare thermoplastic starch (TPS)	234:299	Starch was combined with plasticizers such as glycerol, sorbitol, glycerol/sorbitol and urea/ethanolamine blends by means of high shear extrusion process to prepare thermoplastic starch (TPS).
25439906	5	2	theme	peak	915:918	arg1	intensity					920:928	the peak intensity	911:928	the peak intensity of loss factor with aging time	911:959	Dynamical mechanical analysis (DMA) yields a decrease of the peak intensity of loss factor with aging time.
25439906	2	3	theme	plasticizers	329:340	arg1	Effect					302:307	Effect	302:307	Effect of storage time and plasticizers on the structural stability of melt processed TPS	302:390	Effect of storage time and plasticizers on the structural stability of melt processed TPS was investigated.
25439906	2	4	from	Effect	302:307	arg1	stability					360:368	the structural stability	345:368	the structural stability of melt processed TPS	345:390	Effect of storage time and plasticizers on the structural stability of melt processed TPS was investigated.
25439906	0	5	from	Studies	0:6	arg1	effect					15:20	the effect	11:20	the effect of storage time and plasticizers on the structural variations in thermoplastic starch	11:106	Studies on the effect of storage time and plasticizers on the structural variations in thermoplastic starch.
25439906	7	6	theme	plasticizer	1095:1105	arg1	combinations					1107:1118	different plasticizer combinations	1085:1118	different plasticizer combinations	1085:1118	Thus, through different plasticizer combinations and ageing, starch-based materials with significant differences in tensile properties can be obtained, which may be tuned to meet the requirements of a wide range of applications.
25439906	4	7	theme	storage	840:846	arg1	time					848:851	storage time	840:851	storage time	840:851	XRD analysis highlights major changes in the microstructure of plasticized starch, and dependence of crystalline type and degree of crystallinity mainly on the plasticizer composition and storage time.
25439906	3	8	theme	melt	524:527	arg1	process					539:545	melt extrusion process	524:545	melt extrusion process	524:545	Morphological observation, X-ray diffraction (XRD) and Fourier transform infrared (FTIR) spectroscopy reveal that melt extrusion process is efficient in transforming granular starch into a plasticized starch for all plasticizer compositions.
25439906	4	9	from	changes	682:688	arg1	microstructure					697:710	the microstructure	693:710	the microstructure of plasticized starch	693:732	XRD analysis highlights major changes in the microstructure of plasticized starch, and dependence of crystalline type and degree of crystallinity mainly on the plasticizer composition and storage time.
25439906	6	10	theme	ageing	976:981	arg1	dependent					1029:1037	dependent	1029:1037	dependent	1029:1037	The effect of ageing on tensile strength also appears to be highly dependent on the plasticizer composition.
25439906	6	10	theme	ageing	976:981	arg1	effect					966:971	The effect	962:971	The effect of ageing on tensile strength	962:1001	The effect of ageing on tensile strength also appears to be highly dependent on the plasticizer composition.
25439906	3	11	dep	Fourier	465:471	arg1	transform					473:481	transform	473:481	transform infrared (FTIR) spectroscopy	473:510	Morphological observation, X-ray diffraction (XRD) and Fourier transform infrared (FTIR) spectroscopy reveal that melt extrusion process is efficient in transforming granular starch into a plasticized starch for all plasticizer compositions.
25439906	3	12	theme	extrusion	529:537	arg1	process					539:545	melt extrusion process	524:545	melt extrusion process	524:545	Morphological observation, X-ray diffraction (XRD) and Fourier transform infrared (FTIR) spectroscopy reveal that melt extrusion process is efficient in transforming granular starch into a plasticized starch for all plasticizer compositions.
25439906	7	13	from	differences	1172:1182	arg1	properties					1195:1204	tensile properties	1187:1204	tensile properties	1187:1204	Thus, through different plasticizer combinations and ageing, starch-based materials with significant differences in tensile properties can be obtained, which may be tuned to meet the requirements of a wide range of applications.
25439906	5	14	with	intensity	920:928	arg1	time					956:959	aging time	950:959	aging time	950:959	Dynamical mechanical analysis (DMA) yields a decrease of the peak intensity of loss factor with aging time.
25439906	7	15	with	materials	1145:1153	arg1	differences					1172:1182	significant differences	1160:1182	significant differences in tensile properties	1160:1204	Thus, through different plasticizer combinations and ageing, starch-based materials with significant differences in tensile properties can be obtained, which may be tuned to meet the requirements of a wide range of applications.
25439906	4	16	theme	major	676:680	arg1	changes					682:688	major changes	676:688	major changes in the microstructure of plasticized starch	676:732	XRD analysis highlights major changes in the microstructure of plasticized starch, and dependence of crystalline type and degree of crystallinity mainly on the plasticizer composition and storage time.
25439906	2	17	theme	processed	378:386	arg1	TPS					388:390	melt processed TPS	373:390	melt processed TPS	373:390	Effect of storage time and plasticizers on the structural stability of melt processed TPS was investigated.
25439906	0	18	theme	thermoplastic	87:99	arg1	starch					101:106	thermoplastic starch	87:106	thermoplastic starch	87:106	Studies on the effect of storage time and plasticizers on the structural variations in thermoplastic starch.
25439906	6	19	theme	tensile	986:992	arg1	strength					994:1001	tensile strength	986:1001	tensile strength	986:1001	The effect of ageing on tensile strength also appears to be highly dependent on the plasticizer composition.
25439906	3	20	dep	infrared	483:490	arg1	FTIR					493:496	FTIR	493:496	FTIR	493:496	Morphological observation, X-ray diffraction (XRD) and Fourier transform infrared (FTIR) spectroscopy reveal that melt extrusion process is efficient in transforming granular starch into a plasticized starch for all plasticizer compositions.
25439906	2	21	theme	melt	373:376	arg1	TPS					388:390	melt processed TPS	373:390	melt processed TPS	373:390	Effect of storage time and plasticizers on the structural stability of melt processed TPS was investigated.
25439906	4	22	theme	XRD	652:654	arg1	analysis					656:663	XRD analysis	652:663	XRD analysis	652:663	XRD analysis highlights major changes in the microstructure of plasticized starch, and dependence of crystalline type and degree of crystallinity mainly on the plasticizer composition and storage time.
25439906	5	23	theme	loss	933:936	arg1	factor					938:943	loss factor	933:943	loss factor	933:943	Dynamical mechanical analysis (DMA) yields a decrease of the peak intensity of loss factor with aging time.
25439906	3	24	theme	Morphological	410:422	arg1	observation					424:434	Morphological observation	410:434	Morphological observation	410:434	Morphological observation, X-ray diffraction (XRD) and Fourier transform infrared (FTIR) spectroscopy reveal that melt extrusion process is efficient in transforming granular starch into a plasticized starch for all plasticizer compositions.
25439906	7	25	theme	tensile	1187:1193	arg1	properties					1195:1204	tensile properties	1187:1204	tensile properties	1187:1204	Thus, through different plasticizer combinations and ageing, starch-based materials with significant differences in tensile properties can be obtained, which may be tuned to meet the requirements of a wide range of applications.
25439906	0	26	from	variations	73:82	arg1	starch					101:106	thermoplastic starch	87:106	thermoplastic starch	87:106	Studies on the effect of storage time and plasticizers on the structural variations in thermoplastic starch.
25439906	4	27	from	dependence	739:748	arg1	microstructure					697:710	the microstructure	693:710	the microstructure of plasticized starch	693:732	XRD analysis highlights major changes in the microstructure of plasticized starch, and dependence of crystalline type and degree of crystallinity mainly on the plasticizer composition and storage time.
25439906	5	28	theme	factor	938:943	arg1	intensity					920:928	the peak intensity	911:928	the peak intensity of loss factor with aging time	911:959	Dynamical mechanical analysis (DMA) yields a decrease of the peak intensity of loss factor with aging time.
25439906	7	29	theme	significant	1160:1170	arg1	differences					1172:1182	significant differences	1160:1182	significant differences in tensile properties	1160:1204	Thus, through different plasticizer combinations and ageing, starch-based materials with significant differences in tensile properties can be obtained, which may be tuned to meet the requirements of a wide range of applications.
25439906	1	30	theme	thermoplastic	274:286	arg1	starch					288:293	thermoplastic starch	274:293	thermoplastic starch (TPS)	274:299	Starch was combined with plasticizers such as glycerol, sorbitol, glycerol/sorbitol and urea/ethanolamine blends by means of high shear extrusion process to prepare thermoplastic starch (TPS).
25439906	1	30	theme	thermoplastic	274:286	arg1	TPS					296:298	TPS	296:298	TPS	296:298	Starch was combined with plasticizers such as glycerol, sorbitol, glycerol/sorbitol and urea/ethanolamine blends by means of high shear extrusion process to prepare thermoplastic starch (TPS).
25439906	4	31	theme	crystalline	753:763	arg1	type					765:768	crystalline type	753:768	crystalline type	753:768	XRD analysis highlights major changes in the microstructure of plasticized starch, and dependence of crystalline type and degree of crystallinity mainly on the plasticizer composition and storage time.
25439906	7	32	theme	different	1085:1093	arg1	combinations					1107:1118	different plasticizer combinations	1085:1118	different plasticizer combinations	1085:1118	Thus, through different plasticizer combinations and ageing, starch-based materials with significant differences in tensile properties can be obtained, which may be tuned to meet the requirements of a wide range of applications.
25439906	7	33	theme	applications	1286:1297	arg1	range					1277:1281	a wide range	1270:1281	a wide range of applications	1270:1297	Thus, through different plasticizer combinations and ageing, starch-based materials with significant differences in tensile properties can be obtained, which may be tuned to meet the requirements of a wide range of applications.
25439906	5	34	theme	aging	950:954	arg1	time					956:959	aging time	950:959	aging time	950:959	Dynamical mechanical analysis (DMA) yields a decrease of the peak intensity of loss factor with aging time.
25439906	0	35	theme	storage	25:31	arg1	time					33:36	storage time	25:36	storage time	25:36	Studies on the effect of storage time and plasticizers on the structural variations in thermoplastic starch.
25439906	3	36	theme	infrared	483:490	arg1	spectroscopy					499:510	infrared (FTIR) spectroscopy	483:510	infrared (FTIR) spectroscopy	483:510	Morphological observation, X-ray diffraction (XRD) and Fourier transform infrared (FTIR) spectroscopy reveal that melt extrusion process is efficient in transforming granular starch into a plasticized starch for all plasticizer compositions.
25439906	6	37	from	effect	966:971	arg1	strength					994:1001	tensile strength	986:1001	tensile strength	986:1001	The effect of ageing on tensile strength also appears to be highly dependent on the plasticizer composition.
25439906	7	38	theme	range	1277:1281	arg1	requirements					1254:1265	the requirements	1250:1265	the requirements of a wide range of applications	1250:1297	Thus, through different plasticizer combinations and ageing, starch-based materials with significant differences in tensile properties can be obtained, which may be tuned to meet the requirements of a wide range of applications.
25439906	5	39	theme	Dynamical	854:862	arg1	DMA					885:887	DMA	885:887	DMA	885:887	Dynamical mechanical analysis (DMA) yields a decrease of the peak intensity of loss factor with aging time.
25439906	5	39	theme	Dynamical	854:862	arg1	analysis					875:882	Dynamical mechanical analysis	854:882	Dynamical mechanical analysis (DMA)	854:888	Dynamical mechanical analysis (DMA) yields a decrease of the peak intensity of loss factor with aging time.
25439906	6	40	theme	plasticizer	1046:1056	arg1	composition					1058:1068	the plasticizer composition	1042:1068	the plasticizer composition	1042:1068	The effect of ageing on tensile strength also appears to be highly dependent on the plasticizer composition.
25439906	4	41	theme	type	765:768	arg1	changes					682:688	major changes	676:688	major changes in the microstructure of plasticized starch	676:732	XRD analysis highlights major changes in the microstructure of plasticized starch, and dependence of crystalline type and degree of crystallinity mainly on the plasticizer composition and storage time.
25439906	4	41	theme	type	765:768	arg1	degree					774:779	degree	774:779	degree of crystallinity	774:796	XRD analysis highlights major changes in the microstructure of plasticized starch, and dependence of crystalline type and degree of crystallinity mainly on the plasticizer composition and storage time.
25439906	4	41	theme	type	765:768	arg1	dependence					739:748	dependence	739:748	dependence of crystalline type	739:768	XRD analysis highlights major changes in the microstructure of plasticized starch, and dependence of crystalline type and degree of crystallinity mainly on the plasticizer composition and storage time.
25439906	4	42	theme	plasticizer	812:822	arg1	composition					824:834	the plasticizer composition	808:834	the plasticizer composition	808:834	XRD analysis highlights major changes in the microstructure of plasticized starch, and dependence of crystalline type and degree of crystallinity mainly on the plasticizer composition and storage time.
25439906	5	43	theme	mechanical	864:873	arg1	DMA					885:887	DMA	885:887	DMA	885:887	Dynamical mechanical analysis (DMA) yields a decrease of the peak intensity of loss factor with aging time.
25439906	5	43	theme	mechanical	864:873	arg1	analysis					875:882	Dynamical mechanical analysis	854:882	Dynamical mechanical analysis (DMA)	854:888	Dynamical mechanical analysis (DMA) yields a decrease of the peak intensity of loss factor with aging time.
25439906	2	44	theme	TPS	388:390	arg1	stability					360:368	the structural stability	345:368	the structural stability of melt processed TPS	345:390	Effect of storage time and plasticizers on the structural stability of melt processed TPS was investigated.
25439906	3	45	theme	plasticizer	626:636	arg1	compositions					638:649	all plasticizer compositions	622:649	all plasticizer compositions	622:649	Morphological observation, X-ray diffraction (XRD) and Fourier transform infrared (FTIR) spectroscopy reveal that melt extrusion process is efficient in transforming granular starch into a plasticized starch for all plasticizer compositions.
25439906	1	46	dep	glycerol	155:162	arg1	blends					215:220	blends	215:220	blends	215:220	Starch was combined with plasticizers such as glycerol, sorbitol, glycerol/sorbitol and urea/ethanolamine blends by means of high shear extrusion process to prepare thermoplastic starch (TPS).
25439906	4	47	theme	plasticized	715:725	arg1	starch					727:732	plasticized starch	715:732	plasticized starch	715:732	XRD analysis highlights major changes in the microstructure of plasticized starch, and dependence of crystalline type and degree of crystallinity mainly on the plasticizer composition and storage time.
25439906	0	48	theme	time	33:36	arg1	effect					15:20	the effect	11:20	the effect of storage time and plasticizers on the structural variations in thermoplastic starch	11:106	Studies on the effect of storage time and plasticizers on the structural variations in thermoplastic starch.
25439906	3	49	theme	granular	576:583	arg1	starch					585:590	granular starch	576:590	granular starch	576:590	Morphological observation, X-ray diffraction (XRD) and Fourier transform infrared (FTIR) spectroscopy reveal that melt extrusion process is efficient in transforming granular starch into a plasticized starch for all plasticizer compositions.
25439906	2	50	theme	time	320:323	arg1	Effect					302:307	Effect	302:307	Effect of storage time and plasticizers on the structural stability of melt processed TPS	302:390	Effect of storage time and plasticizers on the structural stability of melt processed TPS was investigated.
25439906	7	51	theme	starch-based	1132:1143	arg1	materials					1145:1153	starch-based materials	1132:1153	starch-based materials with significant differences in tensile properties	1132:1204	Thus, through different plasticizer combinations and ageing, starch-based materials with significant differences in tensile properties can be obtained, which may be tuned to meet the requirements of a wide range of applications.
25439906	4	52	from	degree	774:779	arg1	microstructure					697:710	the microstructure	693:710	the microstructure of plasticized starch	693:732	XRD analysis highlights major changes in the microstructure of plasticized starch, and dependence of crystalline type and degree of crystallinity mainly on the plasticizer composition and storage time.
25439906	2	53	theme	storage	312:318	arg1	time					320:323	storage time	312:323	storage time	312:323	Effect of storage time and plasticizers on the structural stability of melt processed TPS was investigated.
25439906	7	54	theme	wide	1272:1275	arg1	range					1277:1281	a wide range	1270:1281	a wide range of applications	1270:1297	Thus, through different plasticizer combinations and ageing, starch-based materials with significant differences in tensile properties can be obtained, which may be tuned to meet the requirements of a wide range of applications.
25439906	4	55	theme	crystallinity	784:796	arg1	changes					682:688	major changes	676:688	major changes in the microstructure of plasticized starch	676:732	XRD analysis highlights major changes in the microstructure of plasticized starch, and dependence of crystalline type and degree of crystallinity mainly on the plasticizer composition and storage time.
25439906	4	55	theme	crystallinity	784:796	arg1	degree					774:779	degree	774:779	degree of crystallinity	774:796	XRD analysis highlights major changes in the microstructure of plasticized starch, and dependence of crystalline type and degree of crystallinity mainly on the plasticizer composition and storage time.
25439906	4	55	theme	crystallinity	784:796	arg1	dependence					739:748	dependence	739:748	dependence of crystalline type	739:768	XRD analysis highlights major changes in the microstructure of plasticized starch, and dependence of crystalline type and degree of crystallinity mainly on the plasticizer composition and storage time.
25439906	4	56	theme	starch	727:732	arg1	microstructure					697:710	the microstructure	693:710	the microstructure of plasticized starch	693:732	XRD analysis highlights major changes in the microstructure of plasticized starch, and dependence of crystalline type and degree of crystallinity mainly on the plasticizer composition and storage time.
25439906	5	57	theme	intensity	920:928	arg1	decrease					899:906	a decrease	897:906	a decrease of the peak intensity of loss factor with aging time	897:959	Dynamical mechanical analysis (DMA) yields a decrease of the peak intensity of loss factor with aging time.
25439906	1	58	dep	extrusion	245:253	arg1	process					255:261	process	255:261	process	255:261	Starch was combined with plasticizers such as glycerol, sorbitol, glycerol/sorbitol and urea/ethanolamine blends by means of high shear extrusion process to prepare thermoplastic starch (TPS).
25439906	2	59	theme	structural	349:358	arg1	stability					360:368	the structural stability	345:368	the structural stability of melt processed TPS	345:390	Effect of storage time and plasticizers on the structural stability of melt processed TPS was investigated.
25439906	3	60	theme	X-ray	437:441	arg1	XRD					456:458	XRD	456:458	XRD	456:458	Morphological observation, X-ray diffraction (XRD) and Fourier transform infrared (FTIR) spectroscopy reveal that melt extrusion process is efficient in transforming granular starch into a plasticized starch for all plasticizer compositions.
25439906	3	60	theme	X-ray	437:441	arg1	diffraction					443:453	X-ray diffraction	437:453	X-ray diffraction (XRD)	437:459	Morphological observation, X-ray diffraction (XRD) and Fourier transform infrared (FTIR) spectroscopy reveal that melt extrusion process is efficient in transforming granular starch into a plasticized starch for all plasticizer compositions.
25439906	0	61	theme	plasticizers	42:53	arg1	effect					15:20	the effect	11:20	the effect of storage time and plasticizers on the structural variations in thermoplastic starch	11:106	Studies on the effect of storage time and plasticizers on the structural variations in thermoplastic starch.
25439906	0	62	from	effect	15:20	arg1	variations					73:82	the structural variations	58:82	the structural variations in thermoplastic starch	58:106	Studies on the effect of storage time and plasticizers on the structural variations in thermoplastic starch.
25439906	3	63	theme	plasticized	599:609	arg1	starch					611:616	a plasticized starch	597:616	a plasticized starch for all plasticizer compositions	597:649	Morphological observation, X-ray diffraction (XRD) and Fourier transform infrared (FTIR) spectroscopy reveal that melt extrusion process is efficient in transforming granular starch into a plasticized starch for all plasticizer compositions.
25439906	1	64	theme	high	234:237	arg1	extrusion					245:253	high shear extrusion	234:253	high shear extrusion process to prepare thermoplastic starch (TPS)	234:299	Starch was combined with plasticizers such as glycerol, sorbitol, glycerol/sorbitol and urea/ethanolamine blends by means of high shear extrusion process to prepare thermoplastic starch (TPS).
25786398	5	0	theme	165	1122:1124	arg1	±					1136:1136	165 ± 54, 565 ± 50 and 993	1122:1147	±	1136:1136	The compressive strengths of them were 165 ± 54, 565 ± 50 and 993 ± 108 kPa, respectively and the strain of the gradient hydrogel decreased along the thickness direction, similar to the natural tissue.
25786398	5	0	theme	165	1122:1124	arg1	strengths					1099:1107	The compressive strengths	1083:1107	The compressive strengths of them	1083:1115	The compressive strengths of them were 165 ± 54, 565 ± 50 and 993 ± 108 kPa, respectively and the strain of the gradient hydrogel decreased along the thickness direction, similar to the natural tissue.
25786398	5	1	theme	them	1112:1115	arg1	±					1136:1136	165 ± 54, 565 ± 50 and 993	1122:1147	±	1136:1136	The compressive strengths of them were 165 ± 54, 565 ± 50 and 993 ± 108 kPa, respectively and the strain of the gradient hydrogel decreased along the thickness direction, similar to the natural tissue.
25786398	5	1	theme	them	1112:1115	arg1	strengths					1099:1107	The compressive strengths	1083:1107	The compressive strengths of them	1083:1115	The compressive strengths of them were 165 ± 54, 565 ± 50 and 993 ± 108 kPa, respectively and the strain of the gradient hydrogel decreased along the thickness direction, similar to the natural tissue.
25786398	2	2	theme	glycidyl	427:434	arg1	GMA					450:452	GMA	450:452	GMA	450:452	Firstly, gelatin and carboxymethyl chitosan were reacted with glycidyl methacrylate (GMA) to obtain methacrylated gelatin (Gtn-GMA) and carboxymethyl chitosan (CS-GMA).
25786398	2	2	theme	glycidyl	427:434	arg1	methacrylate					436:447	glycidyl methacrylate	427:447	glycidyl methacrylate (GMA)	427:453	Firstly, gelatin and carboxymethyl chitosan were reacted with glycidyl methacrylate (GMA) to obtain methacrylated gelatin (Gtn-GMA) and carboxymethyl chitosan (CS-GMA).
25786398	5	3	theme	hydrogel	1204:1211	arg1	strain					1181:1186	the strain	1177:1186	the strain of the gradient hydrogel	1177:1211	The compressive strengths of them were 165 ± 54, 565 ± 50 and 993 ± 108 kPa, respectively and the strain of the gradient hydrogel decreased along the thickness direction, similar to the natural tissue.
25786398	1	4	theme	gradient	306:313	arg1	structure					315:323	the multi-layered gradient structure	288:323	the multi-layered gradient structure of the cartilage-bone interface tissue	288:362	A layered gelatin-chitosan hydrogel with graded composition was prepared via photocrosslinking to simulate the polysaccharide/collagen composition of the natural tissue and mimic the multi-layered gradient structure of the cartilage-bone interface tissue.
25786398	5	5	theme	±	1126:1126	arg1	±					1136:1136	165 ± 54, 565 ± 50 and 993	1122:1147	±	1136:1136	The compressive strengths of them were 165 ± 54, 565 ± 50 and 993 ± 108 kPa, respectively and the strain of the gradient hydrogel decreased along the thickness direction, similar to the natural tissue.
25786398	5	5	theme	±	1126:1126	arg1	strengths					1099:1107	The compressive strengths	1083:1107	The compressive strengths of them	1083:1115	The compressive strengths of them were 165 ± 54, 565 ± 50 and 993 ± 108 kPa, respectively and the strain of the gradient hydrogel decreased along the thickness direction, similar to the natural tissue.
25786398	4	6	theme	superficial	920:930	arg1	layers					955:960	the superficial, transitional and deep layers	916:960	the superficial, transitional and deep layers of the layered hydrogel	916:984	Results suggested that the pore sizes of the superficial, transitional and deep layers of the layered hydrogel were 115 ± 30, 94 ± 34, 51 ± 12 μm, respectively and their porosities were all higher than 80 %.
25786398	3	7	from	solutions	550:558	arg1	degrees					599:605	different methacrylation degrees	574:605	different methacrylation degrees with CS-GMA	574:617	Then, the mixed solutions of Gtn-GMA in different methacrylation degrees with CS-GMA were prepared to form the superficial, transitional and deep layers of the hydrogel, respectively under the irradiation of ultraviolet light, while polyhedral oligomeric silsesquioxane was introduced in the deep layer to improve the mechanical properties.
25786398	3	8	from	degrees	599:605	arg1	solutions					550:558	the mixed solutions	540:558	the mixed solutions of Gtn-GMA in different methacrylation degrees with CS-GMA	540:617	Then, the mixed solutions of Gtn-GMA in different methacrylation degrees with CS-GMA were prepared to form the superficial, transitional and deep layers of the hydrogel, respectively under the irradiation of ultraviolet light, while polyhedral oligomeric silsesquioxane was introduced in the deep layer to improve the mechanical properties.
25786398	6	9	theme	in	1379:1380	arg1	results					1394:1400	the in vivo repair results	1375:1400	the in vivo repair results of osteochondral defect	1375:1424	The in vitro cytotoxicity results showed that the hydrogel had good cytocompatibility and the in vivo repair results of osteochondral defect demonstrated remarkable recovery by using the gradient gelatin-chitosan hydrogel, especially when the hydrogel loading transforming growth factor-β1.
25786398	5	10	theme	compressive	1087:1097	arg1	±					1136:1136	165 ± 54, 565 ± 50 and 993	1122:1147	±	1136:1136	The compressive strengths of them were 165 ± 54, 565 ± 50 and 993 ± 108 kPa, respectively and the strain of the gradient hydrogel decreased along the thickness direction, similar to the natural tissue.
25786398	5	10	theme	compressive	1087:1097	arg1	strengths					1099:1107	The compressive strengths	1083:1107	The compressive strengths of them	1083:1115	The compressive strengths of them were 165 ± 54, 565 ± 50 and 993 ± 108 kPa, respectively and the strain of the gradient hydrogel decreased along the thickness direction, similar to the natural tissue.
25786398	3	11	theme	ultraviolet	742:752	arg1	light					754:758	ultraviolet light	742:758	ultraviolet light	742:758	Then, the mixed solutions of Gtn-GMA in different methacrylation degrees with CS-GMA were prepared to form the superficial, transitional and deep layers of the hydrogel, respectively under the irradiation of ultraviolet light, while polyhedral oligomeric silsesquioxane was introduced in the deep layer to improve the mechanical properties.
25786398	7	12	used	used	1691:1694	arg2	hydrogel					1647:1654	the prepared layered gelatin-chitosan hydrogel	1609:1654	the prepared layered gelatin-chitosan hydrogel in this study	1609:1668	Therefore, it was suggested that the prepared layered gelatin-chitosan hydrogel in this study could be potentially used to promote cartilage-bone interface tissue repair.
25786398	6	13	theme	hydrogel	1528:1535	arg1	loading					1537:1543	the hydrogel loading	1524:1543	the hydrogel loading	1524:1543	The in vitro cytotoxicity results showed that the hydrogel had good cytocompatibility and the in vivo repair results of osteochondral defect demonstrated remarkable recovery by using the gradient gelatin-chitosan hydrogel, especially when the hydrogel loading transforming growth factor-β1.
25786398	6	14	theme	defect	1419:1424	arg1	results					1394:1400	the in vivo repair results	1375:1400	the in vivo repair results of osteochondral defect	1375:1424	The in vitro cytotoxicity results showed that the hydrogel had good cytocompatibility and the in vivo repair results of osteochondral defect demonstrated remarkable recovery by using the gradient gelatin-chitosan hydrogel, especially when the hydrogel loading transforming growth factor-β1.
25786398	4	15	theme	±	995:995	arg1	sizes					907:911	the pore sizes	898:911	the pore sizes of the superficial, transitional and deep layers of the layered hydrogel	898:984	Results suggested that the pore sizes of the superficial, transitional and deep layers of the layered hydrogel were 115 ± 30, 94 ± 34, 51 ± 12 μm, respectively and their porosities were all higher than 80 %.
25786398	4	15	theme	±	995:995	arg1	±					1004:1004	115 ± 30, 94 ± 34, 51 ± 12 μm	991:1019	±	1004:1004	Results suggested that the pore sizes of the superficial, transitional and deep layers of the layered hydrogel were 115 ± 30, 94 ± 34, 51 ± 12 μm, respectively and their porosities were all higher than 80 %.
25786398	3	16	theme	oligomeric	778:787	arg1	silsesquioxane					789:802	polyhedral oligomeric silsesquioxane	767:802	polyhedral oligomeric silsesquioxane	767:802	Then, the mixed solutions of Gtn-GMA in different methacrylation degrees with CS-GMA were prepared to form the superficial, transitional and deep layers of the hydrogel, respectively under the irradiation of ultraviolet light, while polyhedral oligomeric silsesquioxane was introduced in the deep layer to improve the mechanical properties.
25786398	4	17	theme	transitional	933:944	arg1	layers					955:960	the superficial, transitional and deep layers	916:960	the superficial, transitional and deep layers of the layered hydrogel	916:984	Results suggested that the pore sizes of the superficial, transitional and deep layers of the layered hydrogel were 115 ± 30, 94 ± 34, 51 ± 12 μm, respectively and their porosities were all higher than 80 %.
25786398	3	18	from	Gtn-GMA	563:569	arg1	degrees					599:605	different methacrylation degrees	574:605	different methacrylation degrees with CS-GMA	574:617	Then, the mixed solutions of Gtn-GMA in different methacrylation degrees with CS-GMA were prepared to form the superficial, transitional and deep layers of the hydrogel, respectively under the irradiation of ultraviolet light, while polyhedral oligomeric silsesquioxane was introduced in the deep layer to improve the mechanical properties.
25786398	6	19	theme	in	1289:1290	arg1	results					1311:1317	The in vitro cytotoxicity results	1285:1317	The in vitro cytotoxicity results	1285:1317	The in vitro cytotoxicity results showed that the hydrogel had good cytocompatibility and the in vivo repair results of osteochondral defect demonstrated remarkable recovery by using the gradient gelatin-chitosan hydrogel, especially when the hydrogel loading transforming growth factor-β1.
25786398	6	20	theme	cytotoxicity	1298:1309	arg1	results					1311:1317	The in vitro cytotoxicity results	1285:1317	The in vitro cytotoxicity results	1285:1317	The in vitro cytotoxicity results showed that the hydrogel had good cytocompatibility and the in vivo repair results of osteochondral defect demonstrated remarkable recovery by using the gradient gelatin-chitosan hydrogel, especially when the hydrogel loading transforming growth factor-β1.
25786398	6	21	contain	had	1344:1346	arg1	hydrogel					1335:1342	the hydrogel	1331:1342	the hydrogel	1331:1342	The in vitro cytotoxicity results showed that the hydrogel had good cytocompatibility and the in vivo repair results of osteochondral defect demonstrated remarkable recovery by using the gradient gelatin-chitosan hydrogel, especially when the hydrogel loading transforming growth factor-β1.
25786398	6	21	contain	had	1344:1346	arg2	cytocompatibility					1353:1369	good cytocompatibility	1348:1369	good cytocompatibility	1348:1369	The in vitro cytotoxicity results showed that the hydrogel had good cytocompatibility and the in vivo repair results of osteochondral defect demonstrated remarkable recovery by using the gradient gelatin-chitosan hydrogel, especially when the hydrogel loading transforming growth factor-β1.
25786398	3	22	theme	different	574:582	arg1	degrees					599:605	different methacrylation degrees	574:605	different methacrylation degrees with CS-GMA	574:617	Then, the mixed solutions of Gtn-GMA in different methacrylation degrees with CS-GMA were prepared to form the superficial, transitional and deep layers of the hydrogel, respectively under the irradiation of ultraviolet light, while polyhedral oligomeric silsesquioxane was introduced in the deep layer to improve the mechanical properties.
25786398	4	23	theme	pore	902:905	arg1	sizes					907:911	the pore sizes	898:911	the pore sizes of the superficial, transitional and deep layers of the layered hydrogel	898:984	Results suggested that the pore sizes of the superficial, transitional and deep layers of the layered hydrogel were 115 ± 30, 94 ± 34, 51 ± 12 μm, respectively and their porosities were all higher than 80 %.
25786398	4	23	theme	pore	902:905	arg1	±					1004:1004	115 ± 30, 94 ± 34, 51 ± 12 μm	991:1019	±	1004:1004	Results suggested that the pore sizes of the superficial, transitional and deep layers of the layered hydrogel were 115 ± 30, 94 ± 34, 51 ± 12 μm, respectively and their porosities were all higher than 80 %.
25786398	5	24	dep	±	1136:1136	arg1	kPa					1155:1157	± 108 kPa	1149:1157	± 108 kPa	1149:1157	The compressive strengths of them were 165 ± 54, 565 ± 50 and 993 ± 108 kPa, respectively and the strain of the gradient hydrogel decreased along the thickness direction, similar to the natural tissue.
25786398	1	25	with	hydrogel	136:143	arg1	composition					157:167	graded composition	150:167	graded composition	150:167	A layered gelatin-chitosan hydrogel with graded composition was prepared via photocrosslinking to simulate the polysaccharide/collagen composition of the natural tissue and mimic the multi-layered gradient structure of the cartilage-bone interface tissue.
25786398	0	26	theme	layered	17:23	arg1	hydrogel					42:49	layered gelatin-chitosan hydrogel	17:49	layered gelatin-chitosan hydrogel	17:49	Photocrosslinked layered gelatin-chitosan hydrogel with graded compositions for osteochondral defect repair.
25786398	3	27	theme	polyhedral	767:776	arg1	silsesquioxane					789:802	polyhedral oligomeric silsesquioxane	767:802	polyhedral oligomeric silsesquioxane	767:802	Then, the mixed solutions of Gtn-GMA in different methacrylation degrees with CS-GMA were prepared to form the superficial, transitional and deep layers of the hydrogel, respectively under the irradiation of ultraviolet light, while polyhedral oligomeric silsesquioxane was introduced in the deep layer to improve the mechanical properties.
25786398	5	28	theme	±	1149:1149	arg1	kPa					1155:1157	± 108 kPa	1149:1157	± 108 kPa	1149:1157	The compressive strengths of them were 165 ± 54, 565 ± 50 and 993 ± 108 kPa, respectively and the strain of the gradient hydrogel decreased along the thickness direction, similar to the natural tissue.
25786398	7	29	theme	layered	1622:1628	arg1	hydrogel					1647:1654	the prepared layered gelatin-chitosan hydrogel	1609:1654	the prepared layered gelatin-chitosan hydrogel in this study	1609:1668	Therefore, it was suggested that the prepared layered gelatin-chitosan hydrogel in this study could be potentially used to promote cartilage-bone interface tissue repair.
25786398	6	30	dep	in	1379:1380	arg1	vivo					1382:1385	vivo	1382:1385	vivo	1382:1385	The in vitro cytotoxicity results showed that the hydrogel had good cytocompatibility and the in vivo repair results of osteochondral defect demonstrated remarkable recovery by using the gradient gelatin-chitosan hydrogel, especially when the hydrogel loading transforming growth factor-β1.
25786398	1	31	theme	polysaccharide/collagen	220:242	arg1	composition					244:254	the polysaccharide/collagen composition	216:254	the polysaccharide/collagen composition of the natural tissue	216:276	A layered gelatin-chitosan hydrogel with graded composition was prepared via photocrosslinking to simulate the polysaccharide/collagen composition of the natural tissue and mimic the multi-layered gradient structure of the cartilage-bone interface tissue.
25786398	2	32	theme	carboxymethyl	501:513	arg1	CS-GMA					525:530	CS-GMA	525:530	CS-GMA	525:530	Firstly, gelatin and carboxymethyl chitosan were reacted with glycidyl methacrylate (GMA) to obtain methacrylated gelatin (Gtn-GMA) and carboxymethyl chitosan (CS-GMA).
25786398	2	32	theme	carboxymethyl	501:513	arg1	chitosan					515:522	carboxymethyl chitosan	501:522	carboxymethyl chitosan (CS-GMA)	501:531	Firstly, gelatin and carboxymethyl chitosan were reacted with glycidyl methacrylate (GMA) to obtain methacrylated gelatin (Gtn-GMA) and carboxymethyl chitosan (CS-GMA).
25786398	1	33	theme	cartilage-bone	332:345	arg1	tissue					357:362	the cartilage-bone interface tissue	328:362	the cartilage-bone interface tissue	328:362	A layered gelatin-chitosan hydrogel with graded composition was prepared via photocrosslinking to simulate the polysaccharide/collagen composition of the natural tissue and mimic the multi-layered gradient structure of the cartilage-bone interface tissue.
25786398	1	34	theme	layered	111:117	arg1	hydrogel					136:143	A layered gelatin-chitosan hydrogel	109:143	A layered gelatin-chitosan hydrogel with graded composition	109:167	A layered gelatin-chitosan hydrogel with graded composition was prepared via photocrosslinking to simulate the polysaccharide/collagen composition of the natural tissue and mimic the multi-layered gradient structure of the cartilage-bone interface tissue.
25786398	5	35	theme	similar	1254:1260	arg1	direction					1243:1251	the thickness direction	1229:1251	the thickness direction	1229:1251	The compressive strengths of them were 165 ± 54, 565 ± 50 and 993 ± 108 kPa, respectively and the strain of the gradient hydrogel decreased along the thickness direction, similar to the natural tissue.
25786398	1	36	theme	interface	347:355	arg1	tissue					357:362	the cartilage-bone interface tissue	328:362	the cartilage-bone interface tissue	328:362	A layered gelatin-chitosan hydrogel with graded composition was prepared via photocrosslinking to simulate the polysaccharide/collagen composition of the natural tissue and mimic the multi-layered gradient structure of the cartilage-bone interface tissue.
25786398	4	37	theme	hydrogel	977:984	arg1	layers					955:960	the superficial, transitional and deep layers	916:960	the superficial, transitional and deep layers of the layered hydrogel	916:984	Results suggested that the pore sizes of the superficial, transitional and deep layers of the layered hydrogel were 115 ± 30, 94 ± 34, 51 ± 12 μm, respectively and their porosities were all higher than 80 %.
25786398	1	38	theme	gelatin-chitosan	119:134	arg1	hydrogel					136:143	A layered gelatin-chitosan hydrogel	109:143	A layered gelatin-chitosan hydrogel with graded composition	109:167	A layered gelatin-chitosan hydrogel with graded composition was prepared via photocrosslinking to simulate the polysaccharide/collagen composition of the natural tissue and mimic the multi-layered gradient structure of the cartilage-bone interface tissue.
25786398	6	39	theme	gelatin-chitosan	1481:1496	arg1	hydrogel					1498:1505	the gradient gelatin-chitosan hydrogel	1468:1505	the gradient gelatin-chitosan hydrogel	1468:1505	The in vitro cytotoxicity results showed that the hydrogel had good cytocompatibility and the in vivo repair results of osteochondral defect demonstrated remarkable recovery by using the gradient gelatin-chitosan hydrogel, especially when the hydrogel loading transforming growth factor-β1.
25786398	0	40	theme	gelatin-chitosan	25:40	arg1	hydrogel					42:49	layered gelatin-chitosan hydrogel	17:49	layered gelatin-chitosan hydrogel	17:49	Photocrosslinked layered gelatin-chitosan hydrogel with graded compositions for osteochondral defect repair.
25786398	1	41	theme	tissue	357:362	arg1	structure					315:323	the multi-layered gradient structure	288:323	the multi-layered gradient structure of the cartilage-bone interface tissue	288:362	A layered gelatin-chitosan hydrogel with graded composition was prepared via photocrosslinking to simulate the polysaccharide/collagen composition of the natural tissue and mimic the multi-layered gradient structure of the cartilage-bone interface tissue.
25786398	4	42	theme	layered	969:975	arg1	hydrogel					977:984	the layered hydrogel	965:984	the layered hydrogel	965:984	Results suggested that the pore sizes of the superficial, transitional and deep layers of the layered hydrogel were 115 ± 30, 94 ± 34, 51 ± 12 μm, respectively and their porosities were all higher than 80 %.
25786398	3	43	theme	hydrogel	694:701	arg1	layers					680:685	the superficial, transitional and deep layers	641:685	the superficial, transitional and deep layers of the hydrogel	641:701	Then, the mixed solutions of Gtn-GMA in different methacrylation degrees with CS-GMA were prepared to form the superficial, transitional and deep layers of the hydrogel, respectively under the irradiation of ultraviolet light, while polyhedral oligomeric silsesquioxane was introduced in the deep layer to improve the mechanical properties.
25786398	6	44	theme	gradient	1472:1479	arg1	hydrogel					1498:1505	the gradient gelatin-chitosan hydrogel	1468:1505	the gradient gelatin-chitosan hydrogel	1468:1505	The in vitro cytotoxicity results showed that the hydrogel had good cytocompatibility and the in vivo repair results of osteochondral defect demonstrated remarkable recovery by using the gradient gelatin-chitosan hydrogel, especially when the hydrogel loading transforming growth factor-β1.
25786398	0	45	theme	graded	56:61	arg1	compositions					63:74	graded compositions	56:74	graded compositions for osteochondral defect repair	56:106	Photocrosslinked layered gelatin-chitosan hydrogel with graded compositions for osteochondral defect repair.
25786398	3	46	theme	mixed	544:548	arg1	solutions					550:558	the mixed solutions	540:558	the mixed solutions of Gtn-GMA in different methacrylation degrees with CS-GMA	540:617	Then, the mixed solutions of Gtn-GMA in different methacrylation degrees with CS-GMA were prepared to form the superficial, transitional and deep layers of the hydrogel, respectively under the irradiation of ultraviolet light, while polyhedral oligomeric silsesquioxane was introduced in the deep layer to improve the mechanical properties.
25786398	6	47	theme	osteochondral	1405:1417	arg1	defect					1419:1424	osteochondral defect	1405:1424	osteochondral defect	1405:1424	The in vitro cytotoxicity results showed that the hydrogel had good cytocompatibility and the in vivo repair results of osteochondral defect demonstrated remarkable recovery by using the gradient gelatin-chitosan hydrogel, especially when the hydrogel loading transforming growth factor-β1.
25786398	3	48	theme	superficial	645:655	arg1	layers					680:685	the superficial, transitional and deep layers	641:685	the superficial, transitional and deep layers of the hydrogel	641:701	Then, the mixed solutions of Gtn-GMA in different methacrylation degrees with CS-GMA were prepared to form the superficial, transitional and deep layers of the hydrogel, respectively under the irradiation of ultraviolet light, while polyhedral oligomeric silsesquioxane was introduced in the deep layer to improve the mechanical properties.
25786398	5	49	theme	natural	1269:1275	arg1	tissue					1277:1282	the natural tissue	1265:1282	the natural tissue	1265:1282	The compressive strengths of them were 165 ± 54, 565 ± 50 and 993 ± 108 kPa, respectively and the strain of the gradient hydrogel decreased along the thickness direction, similar to the natural tissue.
25786398	1	50	theme	natural	263:269	arg1	tissue					271:276	the natural tissue	259:276	the natural tissue	259:276	A layered gelatin-chitosan hydrogel with graded composition was prepared via photocrosslinking to simulate the polysaccharide/collagen composition of the natural tissue and mimic the multi-layered gradient structure of the cartilage-bone interface tissue.
25786398	1	51	theme	tissue	271:276	arg1	composition					244:254	the polysaccharide/collagen composition	216:254	the polysaccharide/collagen composition of the natural tissue	216:276	A layered gelatin-chitosan hydrogel with graded composition was prepared via photocrosslinking to simulate the polysaccharide/collagen composition of the natural tissue and mimic the multi-layered gradient structure of the cartilage-bone interface tissue.
25786398	3	52	with	degrees	599:605	arg1	CS-GMA					612:617	CS-GMA	612:617	CS-GMA	612:617	Then, the mixed solutions of Gtn-GMA in different methacrylation degrees with CS-GMA were prepared to form the superficial, transitional and deep layers of the hydrogel, respectively under the irradiation of ultraviolet light, while polyhedral oligomeric silsesquioxane was introduced in the deep layer to improve the mechanical properties.
25786398	4	53	theme	layers	955:960	arg1	sizes					907:911	the pore sizes	898:911	the pore sizes of the superficial, transitional and deep layers of the layered hydrogel	898:984	Results suggested that the pore sizes of the superficial, transitional and deep layers of the layered hydrogel were 115 ± 30, 94 ± 34, 51 ± 12 μm, respectively and their porosities were all higher than 80 %.
25786398	4	53	theme	layers	955:960	arg1	±					1004:1004	115 ± 30, 94 ± 34, 51 ± 12 μm	991:1019	±	1004:1004	Results suggested that the pore sizes of the superficial, transitional and deep layers of the layered hydrogel were 115 ± 30, 94 ± 34, 51 ± 12 μm, respectively and their porosities were all higher than 80 %.
25786398	7	54	theme	gelatin-chitosan	1630:1645	arg1	hydrogel					1647:1654	the prepared layered gelatin-chitosan hydrogel	1609:1654	the prepared layered gelatin-chitosan hydrogel in this study	1609:1668	Therefore, it was suggested that the prepared layered gelatin-chitosan hydrogel in this study could be potentially used to promote cartilage-bone interface tissue repair.
25786398	6	55	theme	repair	1387:1392	arg1	results					1394:1400	the in vivo repair results	1375:1400	the in vivo repair results of osteochondral defect	1375:1424	The in vitro cytotoxicity results showed that the hydrogel had good cytocompatibility and the in vivo repair results of osteochondral defect demonstrated remarkable recovery by using the gradient gelatin-chitosan hydrogel, especially when the hydrogel loading transforming growth factor-β1.
25786398	6	56	theme	good	1348:1351	arg1	cytocompatibility					1353:1369	good cytocompatibility	1348:1369	good cytocompatibility	1348:1369	The in vitro cytotoxicity results showed that the hydrogel had good cytocompatibility and the in vivo repair results of osteochondral defect demonstrated remarkable recovery by using the gradient gelatin-chitosan hydrogel, especially when the hydrogel loading transforming growth factor-β1.
25786398	3	57	theme	deep	826:829	arg1	layer					831:835	the deep layer	822:835	the deep layer	822:835	Then, the mixed solutions of Gtn-GMA in different methacrylation degrees with CS-GMA were prepared to form the superficial, transitional and deep layers of the hydrogel, respectively under the irradiation of ultraviolet light, while polyhedral oligomeric silsesquioxane was introduced in the deep layer to improve the mechanical properties.
25786398	4	58	theme	deep	950:953	arg1	layers					955:960	the superficial, transitional and deep layers	916:960	the superficial, transitional and deep layers of the layered hydrogel	916:984	Results suggested that the pore sizes of the superficial, transitional and deep layers of the layered hydrogel were 115 ± 30, 94 ± 34, 51 ± 12 μm, respectively and their porosities were all higher than 80 %.
25786398	6	59	dep	in	1289:1290	arg1	vitro					1292:1296	vitro	1292:1296	vitro	1292:1296	The in vitro cytotoxicity results showed that the hydrogel had good cytocompatibility and the in vivo repair results of osteochondral defect demonstrated remarkable recovery by using the gradient gelatin-chitosan hydrogel, especially when the hydrogel loading transforming growth factor-β1.
25786398	1	60	theme	graded	150:155	arg1	composition					157:167	graded composition	150:167	graded composition	150:167	A layered gelatin-chitosan hydrogel with graded composition was prepared via photocrosslinking to simulate the polysaccharide/collagen composition of the natural tissue and mimic the multi-layered gradient structure of the cartilage-bone interface tissue.
25786398	7	61	from	hydrogel	1647:1654	arg1	study					1664:1668	this study	1659:1668	this study	1659:1668	Therefore, it was suggested that the prepared layered gelatin-chitosan hydrogel in this study could be potentially used to promote cartilage-bone interface tissue repair.
25786398	7	62	theme	prepared	1613:1620	arg1	hydrogel					1647:1654	the prepared layered gelatin-chitosan hydrogel	1609:1654	the prepared layered gelatin-chitosan hydrogel in this study	1609:1668	Therefore, it was suggested that the prepared layered gelatin-chitosan hydrogel in this study could be potentially used to promote cartilage-bone interface tissue repair.
25786398	3	63	theme	deep	675:678	arg1	layers					680:685	the superficial, transitional and deep layers	641:685	the superficial, transitional and deep layers of the hydrogel	641:701	Then, the mixed solutions of Gtn-GMA in different methacrylation degrees with CS-GMA were prepared to form the superficial, transitional and deep layers of the hydrogel, respectively under the irradiation of ultraviolet light, while polyhedral oligomeric silsesquioxane was introduced in the deep layer to improve the mechanical properties.
25786398	7	64	theme	interface	1722:1730	arg1	repair					1739:1744	cartilage-bone interface tissue repair	1707:1744	cartilage-bone interface tissue repair	1707:1744	Therefore, it was suggested that the prepared layered gelatin-chitosan hydrogel in this study could be potentially used to promote cartilage-bone interface tissue repair.
25786398	5	65	theme	thickness	1233:1241	arg1	direction					1243:1251	the thickness direction	1229:1251	the thickness direction	1229:1251	The compressive strengths of them were 165 ± 54, 565 ± 50 and 993 ± 108 kPa, respectively and the strain of the gradient hydrogel decreased along the thickness direction, similar to the natural tissue.
25786398	3	66	theme	methacrylation	584:597	arg1	degrees					599:605	different methacrylation degrees	574:605	different methacrylation degrees with CS-GMA	574:617	Then, the mixed solutions of Gtn-GMA in different methacrylation degrees with CS-GMA were prepared to form the superficial, transitional and deep layers of the hydrogel, respectively under the irradiation of ultraviolet light, while polyhedral oligomeric silsesquioxane was introduced in the deep layer to improve the mechanical properties.
25786398	6	67	theme	growth	1558:1563	arg1	factor-β1					1565:1573	growth factor-β1	1558:1573	growth factor-β1	1558:1573	The in vitro cytotoxicity results showed that the hydrogel had good cytocompatibility and the in vivo repair results of osteochondral defect demonstrated remarkable recovery by using the gradient gelatin-chitosan hydrogel, especially when the hydrogel loading transforming growth factor-β1.
25786398	2	68	theme	carboxymethyl	386:398	arg1	chitosan					400:407	carboxymethyl chitosan	386:407	carboxymethyl chitosan	386:407	Firstly, gelatin and carboxymethyl chitosan were reacted with glycidyl methacrylate (GMA) to obtain methacrylated gelatin (Gtn-GMA) and carboxymethyl chitosan (CS-GMA).
25786398	7	69	theme	tissue	1732:1737	arg1	repair					1739:1744	cartilage-bone interface tissue repair	1707:1744	cartilage-bone interface tissue repair	1707:1744	Therefore, it was suggested that the prepared layered gelatin-chitosan hydrogel in this study could be potentially used to promote cartilage-bone interface tissue repair.
25786398	0	70	theme	defect	94:99	arg1	repair					101:106	osteochondral defect repair	80:106	osteochondral defect repair	80:106	Photocrosslinked layered gelatin-chitosan hydrogel with graded compositions for osteochondral defect repair.
25786398	2	71	theme	methacrylated	465:477	arg1	gelatin					479:485	methacrylated gelatin	465:485	methacrylated gelatin (Gtn-GMA)	465:495	Firstly, gelatin and carboxymethyl chitosan were reacted with glycidyl methacrylate (GMA) to obtain methacrylated gelatin (Gtn-GMA) and carboxymethyl chitosan (CS-GMA).
25786398	2	71	theme	methacrylated	465:477	arg1	Gtn-GMA					488:494	Gtn-GMA	488:494	Gtn-GMA	488:494	Firstly, gelatin and carboxymethyl chitosan were reacted with glycidyl methacrylate (GMA) to obtain methacrylated gelatin (Gtn-GMA) and carboxymethyl chitosan (CS-GMA).
25786398	3	72	theme	transitional	658:669	arg1	layers					680:685	the superficial, transitional and deep layers	641:685	the superficial, transitional and deep layers of the hydrogel	641:701	Then, the mixed solutions of Gtn-GMA in different methacrylation degrees with CS-GMA were prepared to form the superficial, transitional and deep layers of the hydrogel, respectively under the irradiation of ultraviolet light, while polyhedral oligomeric silsesquioxane was introduced in the deep layer to improve the mechanical properties.
25786398	7	73	theme	cartilage-bone	1707:1720	arg1	repair					1739:1744	cartilage-bone interface tissue repair	1707:1744	cartilage-bone interface tissue repair	1707:1744	Therefore, it was suggested that the prepared layered gelatin-chitosan hydrogel in this study could be potentially used to promote cartilage-bone interface tissue repair.
25786398	3	74	theme	Gtn-GMA	563:569	arg1	solutions					550:558	the mixed solutions	540:558	the mixed solutions of Gtn-GMA in different methacrylation degrees with CS-GMA	540:617	Then, the mixed solutions of Gtn-GMA in different methacrylation degrees with CS-GMA were prepared to form the superficial, transitional and deep layers of the hydrogel, respectively under the irradiation of ultraviolet light, while polyhedral oligomeric silsesquioxane was introduced in the deep layer to improve the mechanical properties.
25786398	6	75	theme	remarkable	1439:1448	arg1	recovery					1450:1457	remarkable recovery	1439:1457	remarkable recovery	1439:1457	The in vitro cytotoxicity results showed that the hydrogel had good cytocompatibility and the in vivo repair results of osteochondral defect demonstrated remarkable recovery by using the gradient gelatin-chitosan hydrogel, especially when the hydrogel loading transforming growth factor-β1.
25786398	0	76	theme	osteochondral	80:92	arg1	repair					101:106	osteochondral defect repair	80:106	osteochondral defect repair	80:106	Photocrosslinked layered gelatin-chitosan hydrogel with graded compositions for osteochondral defect repair.
25786398	3	77	theme	light	754:758	arg1	irradiation					727:737	the irradiation	723:737	the irradiation of ultraviolet light	723:758	Then, the mixed solutions of Gtn-GMA in different methacrylation degrees with CS-GMA were prepared to form the superficial, transitional and deep layers of the hydrogel, respectively under the irradiation of ultraviolet light, while polyhedral oligomeric silsesquioxane was introduced in the deep layer to improve the mechanical properties.
25786398	3	78	theme	mechanical	852:861	arg1	properties					863:872	the mechanical properties	848:872	the mechanical properties	848:872	Then, the mixed solutions of Gtn-GMA in different methacrylation degrees with CS-GMA were prepared to form the superficial, transitional and deep layers of the hydrogel, respectively under the irradiation of ultraviolet light, while polyhedral oligomeric silsesquioxane was introduced in the deep layer to improve the mechanical properties.
25786398	5	79	theme	gradient	1195:1202	arg1	hydrogel					1204:1211	the gradient hydrogel	1191:1211	the gradient hydrogel	1191:1211	The compressive strengths of them were 165 ± 54, 565 ± 50 and 993 ± 108 kPa, respectively and the strain of the gradient hydrogel decreased along the thickness direction, similar to the natural tissue.
25786398	1	80	theme	multi-layered	292:304	arg1	structure					315:323	the multi-layered gradient structure	288:323	the multi-layered gradient structure of the cartilage-bone interface tissue	288:362	A layered gelatin-chitosan hydrogel with graded composition was prepared via photocrosslinking to simulate the polysaccharide/collagen composition of the natural tissue and mimic the multi-layered gradient structure of the cartilage-bone interface tissue.
26484597	4	0	theme	mitochondrial	711:723	arg1	activity					725:732	the decreased mitochondrial activity	697:732	the decreased mitochondrial activity	697:732	The in vitro results found that DF1 and DF2 could reverse the decreased mitochondrial activity and the increased LDL release induced by MPP(+) (P<0.01 or P<0.001) which provides further evidence that DF1 and DF2 also exerts a direct protection against the neuronal injury caused by MPP(+).
26484597	1	1	theme	dopaminergic	168:179	arg1	neurons					181:187	dopaminergic neurons	168:187	dopaminergic neurons	168:187	Parkinson's disease (PD) is associated with progressive loss of dopaminergic neurons and more-widespread neuronal changes that cause complex symptoms.
26484597	7	2	contain	had	1298:1300	arg1	fraction					1289:1296	the DF1 fraction	1281:1296	the DF1 fraction	1281:1296	The chemical composition of DF1, DF2 and DF differed markedly, the DF1 fraction had the most complex chemical composition and showed the highest neuron protective activity.
26484597	7	2	contain	had	1298:1300	arg2	composition					1328:1338	the most complex chemical composition	1302:1338	the most complex chemical composition	1302:1338	The chemical composition of DF1, DF2 and DF differed markedly, the DF1 fraction had the most complex chemical composition and showed the highest neuron protective activity.
26484597	0	3	from	relationship	19:30	arg1	neuron					96:101	dopaminergic neuron	83:101	dopaminergic neuron	83:101	Structure-activity relationship of sulfated hetero/galactofucan polysaccharides on dopaminergic neuron.
26484597	4	4	dep	MPP	775:777	arg1	P<0.001					793:799	P<0.001	793:799	P<0.001	793:799	The in vitro results found that DF1 and DF2 could reverse the decreased mitochondrial activity and the increased LDL release induced by MPP(+) (P<0.01 or P<0.001) which provides further evidence that DF1 and DF2 also exerts a direct protection against the neuronal injury caused by MPP(+).
26484597	4	4	dep	MPP	775:777	arg1	P<0.01					783:788	P<0.01	783:788	P<0.01	783:788	The in vitro results found that DF1 and DF2 could reverse the decreased mitochondrial activity and the increased LDL release induced by MPP(+) (P<0.01 or P<0.001) which provides further evidence that DF1 and DF2 also exerts a direct protection against the neuronal injury caused by MPP(+).
26484597	5	5	from	GSH	1051:1053	arg1	mice					1084:1087	MPTP mice	1079:1087	MPTP mice	1079:1087	Furthermore, the administration of samples effectively decreased lipid peroxidation and increased the level/activities of GSH, GSH-PX, MDA and CAT in MPTP mice.
26484597	2	6	theme	galactofucan	386:397	arg1	polysaccharides					399:413	sulfated galactofucan polysaccharides	377:413	sulfated galactofucan polysaccharides (DF2)	377:419	The aim of this study was to investigate the structure-activity relationship of sulfated hetero-polysaccharides (DF1) and sulfated galactofucan polysaccharides (DF2) on dopaminergic neuron in vivo and in vitro.
26484597	2	6	theme	galactofucan	386:397	arg1	DF2					416:418	DF2	416:418	DF2	416:418	The aim of this study was to investigate the structure-activity relationship of sulfated hetero-polysaccharides (DF1) and sulfated galactofucan polysaccharides (DF2) on dopaminergic neuron in vivo and in vitro.
26484597	5	7	from	GSH-PX	1056:1061	arg1	mice					1084:1087	MPTP mice	1079:1087	MPTP mice	1079:1087	Furthermore, the administration of samples effectively decreased lipid peroxidation and increased the level/activities of GSH, GSH-PX, MDA and CAT in MPTP mice.
26484597	8	8	theme	uronic	1446:1451	arg1	acid					1453:1456	uronic acid	1446:1456	uronic acid	1446:1456	These results suggest that diverse monosaccharides and uronic acid might contribute to neuron protective activity.
26484597	6	9	theme	antioxidant	1159:1169	arg1	activity					1171:1178	antioxidant activity	1159:1178	antioxidant activity	1159:1178	Thus, the neuron protective effect may be mediated, in part, through antioxidant activity and the prevention of cell apoptosis.
26484597	3	10	theme	neurons	573:579	arg1	depletion					519:527	the depletion	515:527	the depletion of both DA and TH-, Bcl-2- and Bax-positive neurons in MPTP-induced PD mice	515:603	Treatment with samples significantly ameliorated the depletion of both DA and TH-, Bcl-2- and Bax-positive neurons in MPTP-induced PD mice, DF1 showed the highest activity.
26484597	3	11	theme	PD	597:598	arg1	mice					600:603	MPTP-induced PD mice	584:603	MPTP-induced PD mice	584:603	Treatment with samples significantly ameliorated the depletion of both DA and TH-, Bcl-2- and Bax-positive neurons in MPTP-induced PD mice, DF1 showed the highest activity.
26484597	5	12	theme	MDA	1064:1066	arg1	level/activities					1031:1046	the level/activities	1027:1046	the level/activities of GSH, GSH-PX, MDA and CAT in MPTP mice	1027:1087	Furthermore, the administration of samples effectively decreased lipid peroxidation and increased the level/activities of GSH, GSH-PX, MDA and CAT in MPTP mice.
26484597	6	13	theme	apoptosis	1207:1215	arg1	activity					1171:1178	antioxidant activity	1159:1178	antioxidant activity	1159:1178	Thus, the neuron protective effect may be mediated, in part, through antioxidant activity and the prevention of cell apoptosis.
26484597	6	13	theme	apoptosis	1207:1215	arg1	prevention					1188:1197	the prevention	1184:1197	the prevention of cell apoptosis	1184:1215	Thus, the neuron protective effect may be mediated, in part, through antioxidant activity and the prevention of cell apoptosis.
26484597	2	14	theme	structure-activity	300:317	arg1	relationship					319:330	the structure-activity relationship	296:330	the structure-activity relationship of sulfated hetero-polysaccharides (DF1) and sulfated galactofucan polysaccharides (DF2) on dopaminergic neuron	296:442	The aim of this study was to investigate the structure-activity relationship of sulfated hetero-polysaccharides (DF1) and sulfated galactofucan polysaccharides (DF2) on dopaminergic neuron in vivo and in vitro.
26484597	4	15	theme	decreased	701:709	arg1	activity					725:732	the decreased mitochondrial activity	697:732	the decreased mitochondrial activity	697:732	The in vitro results found that DF1 and DF2 could reverse the decreased mitochondrial activity and the increased LDL release induced by MPP(+) (P<0.01 or P<0.001) which provides further evidence that DF1 and DF2 also exerts a direct protection against the neuronal injury caused by MPP(+).
26484597	6	16	theme	cell	1202:1205	arg1	apoptosis					1207:1215	cell apoptosis	1202:1215	cell apoptosis	1202:1215	Thus, the neuron protective effect may be mediated, in part, through antioxidant activity and the prevention of cell apoptosis.
26484597	7	17	theme	DF2	1251:1253	arg1	composition					1231:1241	The chemical composition	1218:1241	The chemical composition of DF1, DF2 and DF	1218:1260	The chemical composition of DF1, DF2 and DF differed markedly, the DF1 fraction had the most complex chemical composition and showed the highest neuron protective activity.
26484597	7	18	theme	DF	1259:1260	arg1	composition					1231:1241	The chemical composition	1218:1241	The chemical composition of DF1, DF2 and DF	1218:1260	The chemical composition of DF1, DF2 and DF differed markedly, the DF1 fraction had the most complex chemical composition and showed the highest neuron protective activity.
26484597	5	19	theme	CAT	1072:1074	arg1	level/activities					1031:1046	the level/activities	1027:1046	the level/activities of GSH, GSH-PX, MDA and CAT in MPTP mice	1027:1087	Furthermore, the administration of samples effectively decreased lipid peroxidation and increased the level/activities of GSH, GSH-PX, MDA and CAT in MPTP mice.
26484597	3	20	from	depletion	519:527	arg1	mice					600:603	MPTP-induced PD mice	584:603	MPTP-induced PD mice	584:603	Treatment with samples significantly ameliorated the depletion of both DA and TH-, Bcl-2- and Bax-positive neurons in MPTP-induced PD mice, DF1 showed the highest activity.
26484597	3	21	theme	Bax-positive	560:571	arg1	neurons					573:579	Bax-positive neurons	560:579	Bax-positive neurons	560:579	Treatment with samples significantly ameliorated the depletion of both DA and TH-, Bcl-2- and Bax-positive neurons in MPTP-induced PD mice, DF1 showed the highest activity.
26484597	2	22	theme	dopaminergic	424:435	arg1	neuron					437:442	dopaminergic neuron	424:442	dopaminergic neuron	424:442	The aim of this study was to investigate the structure-activity relationship of sulfated hetero-polysaccharides (DF1) and sulfated galactofucan polysaccharides (DF2) on dopaminergic neuron in vivo and in vitro.
26484597	5	23	from	CAT	1072:1074	arg1	mice					1084:1087	MPTP mice	1079:1087	MPTP mice	1079:1087	Furthermore, the administration of samples effectively decreased lipid peroxidation and increased the level/activities of GSH, GSH-PX, MDA and CAT in MPTP mice.
26484597	6	24	theme	protective	1107:1116	arg1	effect					1118:1123	the neuron protective effect	1096:1123	the neuron protective effect	1096:1123	Thus, the neuron protective effect may be mediated, in part, through antioxidant activity and the prevention of cell apoptosis.
26484597	1	25	theme	neuronal	209:216	arg1	changes					218:224	more-widespread neuronal changes	193:224	more-widespread neuronal changes that cause complex symptoms	193:252	Parkinson's disease (PD) is associated with progressive loss of dopaminergic neurons and more-widespread neuronal changes that cause complex symptoms.
26484597	0	26	theme	Structure-activity	0:17	arg1	relationship					19:30	Structure-activity relationship	0:30	Structure-activity relationship of sulfated hetero/galactofucan polysaccharides on dopaminergic neuron	0:101	Structure-activity relationship of sulfated hetero/galactofucan polysaccharides on dopaminergic neuron.
26484597	7	27	theme	highest	1355:1361	arg1	activity					1381:1388	the highest neuron protective activity	1351:1388	the highest neuron protective activity	1351:1388	The chemical composition of DF1, DF2 and DF differed markedly, the DF1 fraction had the most complex chemical composition and showed the highest neuron protective activity.
26484597	2	28	theme	hetero-polysaccharides	344:365	arg1	relationship					319:330	the structure-activity relationship	296:330	the structure-activity relationship of sulfated hetero-polysaccharides (DF1) and sulfated galactofucan polysaccharides (DF2) on dopaminergic neuron	296:442	The aim of this study was to investigate the structure-activity relationship of sulfated hetero-polysaccharides (DF1) and sulfated galactofucan polysaccharides (DF2) on dopaminergic neuron in vivo and in vitro.
26484597	7	29	theme	neuron	1363:1368	arg1	activity					1381:1388	the highest neuron protective activity	1351:1388	the highest neuron protective activity	1351:1388	The chemical composition of DF1, DF2 and DF differed markedly, the DF1 fraction had the most complex chemical composition and showed the highest neuron protective activity.
26484597	0	30	theme	sulfated	35:42	arg1	polysaccharides					64:78	sulfated hetero/galactofucan polysaccharides	35:78	sulfated hetero/galactofucan polysaccharides	35:78	Structure-activity relationship of sulfated hetero/galactofucan polysaccharides on dopaminergic neuron.
26484597	5	31	theme	samples	964:970	arg1	administration					946:959	the administration	942:959	the administration of samples	942:970	Furthermore, the administration of samples effectively decreased lipid peroxidation and increased the level/activities of GSH, GSH-PX, MDA and CAT in MPTP mice.
26484597	2	32	theme	sulfated	335:342	arg1	DF1					368:370	DF1	368:370	DF1	368:370	The aim of this study was to investigate the structure-activity relationship of sulfated hetero-polysaccharides (DF1) and sulfated galactofucan polysaccharides (DF2) on dopaminergic neuron in vivo and in vitro.
26484597	2	32	theme	sulfated	335:342	arg1	hetero-polysaccharides					344:365	sulfated hetero-polysaccharides	335:365	sulfated hetero-polysaccharides (DF1)	335:371	The aim of this study was to investigate the structure-activity relationship of sulfated hetero-polysaccharides (DF1) and sulfated galactofucan polysaccharides (DF2) on dopaminergic neuron in vivo and in vitro.
26484597	7	33	theme	DF1	1246:1248	arg1	composition					1231:1241	The chemical composition	1218:1241	The chemical composition of DF1, DF2 and DF	1218:1260	The chemical composition of DF1, DF2 and DF differed markedly, the DF1 fraction had the most complex chemical composition and showed the highest neuron protective activity.
26484597	4	34	theme	LDL	752:754	arg1	release					756:762	the increased LDL release	738:762	the increased LDL release induced by MPP(+) (P<0.01 or P<0.001) which provides further evidence that DF1 and DF2 also exerts a direct protection against the neuronal injury caused by MPP(+)	738:926	The in vitro results found that DF1 and DF2 could reverse the decreased mitochondrial activity and the increased LDL release induced by MPP(+) (P<0.01 or P<0.001) which provides further evidence that DF1 and DF2 also exerts a direct protection against the neuronal injury caused by MPP(+).
26484597	4	35	theme	neuronal	895:902	arg1	injury					904:909	the neuronal injury	891:909	the neuronal injury caused by MPP(+)	891:926	The in vitro results found that DF1 and DF2 could reverse the decreased mitochondrial activity and the increased LDL release induced by MPP(+) (P<0.01 or P<0.001) which provides further evidence that DF1 and DF2 also exerts a direct protection against the neuronal injury caused by MPP(+).
26484597	5	36	theme	GSH	1051:1053	arg1	level/activities					1031:1046	the level/activities	1027:1046	the level/activities of GSH, GSH-PX, MDA and CAT in MPTP mice	1027:1087	Furthermore, the administration of samples effectively decreased lipid peroxidation and increased the level/activities of GSH, GSH-PX, MDA and CAT in MPTP mice.
26484597	1	37	theme	neurons	181:187	arg1	changes					218:224	more-widespread neuronal changes	193:224	more-widespread neuronal changes that cause complex symptoms	193:252	Parkinson's disease (PD) is associated with progressive loss of dopaminergic neurons and more-widespread neuronal changes that cause complex symptoms.
26484597	1	37	theme	neurons	181:187	arg1	loss					160:163	progressive loss	148:163	progressive loss of dopaminergic neurons	148:187	Parkinson's disease (PD) is associated with progressive loss of dopaminergic neurons and more-widespread neuronal changes that cause complex symptoms.
26484597	8	38	theme	neuron	1478:1483	arg1	activity					1496:1503	neuron protective activity	1478:1503	neuron protective activity	1478:1503	These results suggest that diverse monosaccharides and uronic acid might contribute to neuron protective activity.
26484597	6	39	theme	neuron	1100:1105	arg1	effect					1118:1123	the neuron protective effect	1096:1123	the neuron protective effect	1096:1123	Thus, the neuron protective effect may be mediated, in part, through antioxidant activity and the prevention of cell apoptosis.
26484597	0	40	theme	polysaccharides	64:78	arg1	relationship					19:30	Structure-activity relationship	0:30	Structure-activity relationship of sulfated hetero/galactofucan polysaccharides on dopaminergic neuron	0:101	Structure-activity relationship of sulfated hetero/galactofucan polysaccharides on dopaminergic neuron.
26484597	7	41	theme	chemical	1222:1229	arg1	composition					1231:1241	The chemical composition	1218:1241	The chemical composition of DF1, DF2 and DF	1218:1260	The chemical composition of DF1, DF2 and DF differed markedly, the DF1 fraction had the most complex chemical composition and showed the highest neuron protective activity.
26484597	1	42	theme	complex	237:243	arg1	symptoms					245:252	complex symptoms	237:252	complex symptoms	237:252	Parkinson's disease (PD) is associated with progressive loss of dopaminergic neurons and more-widespread neuronal changes that cause complex symptoms.
26484597	0	43	theme	hetero/galactofucan	44:62	arg1	polysaccharides					64:78	sulfated hetero/galactofucan polysaccharides	35:78	sulfated hetero/galactofucan polysaccharides	35:78	Structure-activity relationship of sulfated hetero/galactofucan polysaccharides on dopaminergic neuron.
26484597	7	44	theme	complex	1311:1317	arg1	composition					1328:1338	the most complex chemical composition	1302:1338	the most complex chemical composition	1302:1338	The chemical composition of DF1, DF2 and DF differed markedly, the DF1 fraction had the most complex chemical composition and showed the highest neuron protective activity.
26484597	5	45	theme	GSH-PX	1056:1061	arg1	level/activities					1031:1046	the level/activities	1027:1046	the level/activities of GSH, GSH-PX, MDA and CAT in MPTP mice	1027:1087	Furthermore, the administration of samples effectively decreased lipid peroxidation and increased the level/activities of GSH, GSH-PX, MDA and CAT in MPTP mice.
26484597	3	46	theme	highest	621:627	arg1	activity					629:636	the highest activity	617:636	the highest activity	617:636	Treatment with samples significantly ameliorated the depletion of both DA and TH-, Bcl-2- and Bax-positive neurons in MPTP-induced PD mice, DF1 showed the highest activity.
26484597	0	47	theme	dopaminergic	83:94	arg1	neuron					96:101	dopaminergic neuron	83:101	dopaminergic neuron	83:101	Structure-activity relationship of sulfated hetero/galactofucan polysaccharides on dopaminergic neuron.
26484597	8	48	theme	protective	1485:1494	arg1	activity					1496:1503	neuron protective activity	1478:1503	neuron protective activity	1478:1503	These results suggest that diverse monosaccharides and uronic acid might contribute to neuron protective activity.
26484597	4	49	theme	further	817:823	arg1	evidence					825:832	further evidence that DF1 and DF2 also exerts a direct protection against the neuronal injury caused by MPP(+)	817:926	further evidence that DF1 and DF2 also exerts a direct protection against the neuronal injury caused by MPP(+)	817:926	The in vitro results found that DF1 and DF2 could reverse the decreased mitochondrial activity and the increased LDL release induced by MPP(+) (P<0.01 or P<0.001) which provides further evidence that DF1 and DF2 also exerts a direct protection against the neuronal injury caused by MPP(+).
26484597	7	50	theme	protective	1370:1379	arg1	activity					1381:1388	the highest neuron protective activity	1351:1388	the highest neuron protective activity	1351:1388	The chemical composition of DF1, DF2 and DF differed markedly, the DF1 fraction had the most complex chemical composition and showed the highest neuron protective activity.
26484597	2	51	theme	sulfated	377:384	arg1	polysaccharides					399:413	sulfated galactofucan polysaccharides	377:413	sulfated galactofucan polysaccharides (DF2)	377:419	The aim of this study was to investigate the structure-activity relationship of sulfated hetero-polysaccharides (DF1) and sulfated galactofucan polysaccharides (DF2) on dopaminergic neuron in vivo and in vitro.
26484597	2	51	theme	sulfated	377:384	arg1	DF2					416:418	DF2	416:418	DF2	416:418	The aim of this study was to investigate the structure-activity relationship of sulfated hetero-polysaccharides (DF1) and sulfated galactofucan polysaccharides (DF2) on dopaminergic neuron in vivo and in vitro.
26484597	5	52	from	mice	1084:1087	arg1	level/activities					1031:1046	the level/activities	1027:1046	the level/activities of GSH, GSH-PX, MDA and CAT in MPTP mice	1027:1087	Furthermore, the administration of samples effectively decreased lipid peroxidation and increased the level/activities of GSH, GSH-PX, MDA and CAT in MPTP mice.
26484597	4	53	theme	direct	865:870	arg1	protection					872:881	a direct protection	863:881	a direct protection against the neuronal injury caused by MPP(+)	863:926	The in vitro results found that DF1 and DF2 could reverse the decreased mitochondrial activity and the increased LDL release induced by MPP(+) (P<0.01 or P<0.001) which provides further evidence that DF1 and DF2 also exerts a direct protection against the neuronal injury caused by MPP(+).
26484597	4	54	dep	in	643:644	arg1	vitro					646:650	vitro	646:650	vitro	646:650	The in vitro results found that DF1 and DF2 could reverse the decreased mitochondrial activity and the increased LDL release induced by MPP(+) (P<0.01 or P<0.001) which provides further evidence that DF1 and DF2 also exerts a direct protection against the neuronal injury caused by MPP(+).
26484597	3	55	theme	TH-	544:546	arg1	depletion					519:527	the depletion	515:527	the depletion of both DA and TH-, Bcl-2- and Bax-positive neurons in MPTP-induced PD mice	515:603	Treatment with samples significantly ameliorated the depletion of both DA and TH-, Bcl-2- and Bax-positive neurons in MPTP-induced PD mice, DF1 showed the highest activity.
26484597	3	56	theme	DA	537:538	arg1	depletion					519:527	the depletion	515:527	the depletion of both DA and TH-, Bcl-2- and Bax-positive neurons in MPTP-induced PD mice	515:603	Treatment with samples significantly ameliorated the depletion of both DA and TH-, Bcl-2- and Bax-positive neurons in MPTP-induced PD mice, DF1 showed the highest activity.
26484597	3	57	with	Treatment	466:474	arg1	samples					481:487	samples	481:487	samples	481:487	Treatment with samples significantly ameliorated the depletion of both DA and TH-, Bcl-2- and Bax-positive neurons in MPTP-induced PD mice, DF1 showed the highest activity.
26484597	5	58	theme	lipid	994:998	arg1	peroxidation					1000:1011	lipid peroxidation	994:1011	lipid peroxidation	994:1011	Furthermore, the administration of samples effectively decreased lipid peroxidation and increased the level/activities of GSH, GSH-PX, MDA and CAT in MPTP mice.
26484597	4	59	theme	increased	742:750	arg1	release					756:762	the increased LDL release	738:762	the increased LDL release induced by MPP(+) (P<0.01 or P<0.001) which provides further evidence that DF1 and DF2 also exerts a direct protection against the neuronal injury caused by MPP(+)	738:926	The in vitro results found that DF1 and DF2 could reverse the decreased mitochondrial activity and the increased LDL release induced by MPP(+) (P<0.01 or P<0.001) which provides further evidence that DF1 and DF2 also exerts a direct protection against the neuronal injury caused by MPP(+).
26484597	3	60	theme	MPTP-induced	584:595	arg1	mice					600:603	MPTP-induced PD mice	584:603	MPTP-induced PD mice	584:603	Treatment with samples significantly ameliorated the depletion of both DA and TH-, Bcl-2- and Bax-positive neurons in MPTP-induced PD mice, DF1 showed the highest activity.
26484597	7	61	theme	chemical	1319:1326	arg1	composition					1328:1338	the most complex chemical composition	1302:1338	the most complex chemical composition	1302:1338	The chemical composition of DF1, DF2 and DF differed markedly, the DF1 fraction had the most complex chemical composition and showed the highest neuron protective activity.
26484597	8	62	theme	diverse	1418:1424	arg1	monosaccharides					1426:1440	diverse monosaccharides	1418:1440	diverse monosaccharides	1418:1440	These results suggest that diverse monosaccharides and uronic acid might contribute to neuron protective activity.
26484597	7	63	theme	DF1	1285:1287	arg1	fraction					1289:1296	the DF1 fraction	1281:1296	the DF1 fraction	1281:1296	The chemical composition of DF1, DF2 and DF differed markedly, the DF1 fraction had the most complex chemical composition and showed the highest neuron protective activity.
26484597	1	64	theme	progressive	148:158	arg1	loss					160:163	progressive loss	148:163	progressive loss of dopaminergic neurons	148:187	Parkinson's disease (PD) is associated with progressive loss of dopaminergic neurons and more-widespread neuronal changes that cause complex symptoms.
26484597	2	65	from	relationship	319:330	arg1	neuron					437:442	dopaminergic neuron	424:442	dopaminergic neuron	424:442	The aim of this study was to investigate the structure-activity relationship of sulfated hetero-polysaccharides (DF1) and sulfated galactofucan polysaccharides (DF2) on dopaminergic neuron in vivo and in vitro.
26484597	5	66	from	level/activities	1031:1046	arg1	mice					1084:1087	MPTP mice	1079:1087	MPTP mice	1079:1087	Furthermore, the administration of samples effectively decreased lipid peroxidation and increased the level/activities of GSH, GSH-PX, MDA and CAT in MPTP mice.
26484597	5	67	from	MDA	1064:1066	arg1	mice					1084:1087	MPTP mice	1079:1087	MPTP mice	1079:1087	Furthermore, the administration of samples effectively decreased lipid peroxidation and increased the level/activities of GSH, GSH-PX, MDA and CAT in MPTP mice.
26484597	2	68	theme	study	271:275	arg1	aim					259:261	The aim	255:261	The aim of this study	255:275	The aim of this study was to investigate the structure-activity relationship of sulfated hetero-polysaccharides (DF1) and sulfated galactofucan polysaccharides (DF2) on dopaminergic neuron in vivo and in vitro.
26484597	2	69	theme	polysaccharides	399:413	arg1	relationship					319:330	the structure-activity relationship	296:330	the structure-activity relationship of sulfated hetero-polysaccharides (DF1) and sulfated galactofucan polysaccharides (DF2) on dopaminergic neuron	296:442	The aim of this study was to investigate the structure-activity relationship of sulfated hetero-polysaccharides (DF1) and sulfated galactofucan polysaccharides (DF2) on dopaminergic neuron in vivo and in vitro.
26484597	1	70	theme	more-widespread	193:207	arg1	changes					218:224	more-widespread neuronal changes	193:224	more-widespread neuronal changes that cause complex symptoms	193:252	Parkinson's disease (PD) is associated with progressive loss of dopaminergic neurons and more-widespread neuronal changes that cause complex symptoms.
26484597	4	71	theme	in	643:644	arg1	results					652:658	The in vitro results	639:658	The in vitro results	639:658	The in vitro results found that DF1 and DF2 could reverse the decreased mitochondrial activity and the increased LDL release induced by MPP(+) (P<0.01 or P<0.001) which provides further evidence that DF1 and DF2 also exerts a direct protection against the neuronal injury caused by MPP(+).
26484597	5	72	theme	MPTP	1079:1082	arg1	mice					1084:1087	MPTP mice	1079:1087	MPTP mice	1079:1087	Furthermore, the administration of samples effectively decreased lipid peroxidation and increased the level/activities of GSH, GSH-PX, MDA and CAT in MPTP mice.
26484597	3	73	theme	Bcl-2-	549:554	arg1	depletion					519:527	the depletion	515:527	the depletion of both DA and TH-, Bcl-2- and Bax-positive neurons in MPTP-induced PD mice	515:603	Treatment with samples significantly ameliorated the depletion of both DA and TH-, Bcl-2- and Bax-positive neurons in MPTP-induced PD mice, DF1 showed the highest activity.
24412921	0	0	theme	enzymatic	83:91	arg1	digestibility					93:105	enzymatic digestibility	83:105	enzymatic digestibility	83:105	Effects of compositional changes of AFEX-treated and H-AFEX-treated corn stover on enzymatic digestibility.
24412921	6	1	theme	ammonia	894:900	arg1	30wt					921:924	0.5 (w/w) 30wt	911:924	0.5 (w/w) 30wt	911:924	The maximum glucan and xylan digestibility of H-AFEX process were 87.78% and 90.64%, respectively, and were obtained using 0.7 (w/w) water loading, 1.0 (w/w) ammonia loading, 0.5 (w/w) 30wt.
24412921	6	1	theme	ammonia	894:900	arg1	loading					875:881	0.7 (w/w) water loading	859:881	0.7 (w/w) water loading	859:881	The maximum glucan and xylan digestibility of H-AFEX process were 87.78% and 90.64%, respectively, and were obtained using 0.7 (w/w) water loading, 1.0 (w/w) ammonia loading, 0.5 (w/w) 30wt.
24412921	6	1	theme	ammonia	894:900	arg1	loading					902:908	1.0 (w/w) ammonia loading	884:908	1.0 (w/w) ammonia loading	884:908	The maximum glucan and xylan digestibility of H-AFEX process were 87.78% and 90.64%, respectively, and were obtained using 0.7 (w/w) water loading, 1.0 (w/w) ammonia loading, 0.5 (w/w) 30wt.
24412921	5	2	theme	enzymatic	680:688	arg1	hydrolysis					690:699	enzymatic hydrolysis	680:699	enzymatic hydrolysis	680:699	Compared with AFEX pretreatment, the H-AFEX process enhanced delignification and enzymatic hydrolysis yields of both glucose and xylose.
24412921	1	3	theme	residues	152:159	arg1	residues					152:159	the main agricultural residues	130:159	the main agricultural residues being considered as a cellulosic ethanol feedstock	130:210	Corn stover is one of the main agricultural residues being considered as a cellulosic ethanol feedstock.
24412921	1	3	theme	residues	152:159	arg1	one					123:125	one	123:125	one	123:125	Corn stover is one of the main agricultural residues being considered as a cellulosic ethanol feedstock.
24412921	5	4	theme	hydrolysis	690:699	arg1	yields					701:706	delignification and enzymatic hydrolysis yields	660:706	delignification and enzymatic hydrolysis yields of both glucose and xylose	660:733	Compared with AFEX pretreatment, the H-AFEX process enhanced delignification and enzymatic hydrolysis yields of both glucose and xylose.
24412921	8	5	theme	stover	1102:1107	arg1	saccharification					1077:1092	the enzymatic saccharification	1063:1092	the enzymatic saccharification of corn stover for bioethanol production	1063:1133	The results of the present work show that H-AFEX is a feasible pretreatment to improve the enzymatic saccharification of corn stover for bioethanol production.
24412921	0	6	from	Effects	0:6	arg1	digestibility					93:105	enzymatic digestibility	83:105	enzymatic digestibility	83:105	Effects of compositional changes of AFEX-treated and H-AFEX-treated corn stover on enzymatic digestibility.
24412921	7	7	theme	%	926:926	arg1	peroxide					937:944	% hydrogen peroxide loading, and 130°C	926:963	peroxide	937:944	% hydrogen peroxide loading, and 130°C for 10min.
24412921	3	8	theme	compositional	386:398	arg1	changes					400:406	The compositional changes	382:406	The compositional changes	382:406	The compositional changes and enzymatic digestibility of AFEX-treated and H-AFEX-treated biomass were investigated.
24412921	8	9	theme	corn	1097:1100	arg1	stover					1102:1107	corn stover	1097:1107	corn stover	1097:1107	The results of the present work show that H-AFEX is a feasible pretreatment to improve the enzymatic saccharification of corn stover for bioethanol production.
24412921	2	10	dep	converting	280:289	arg1	both					326:329	both	326:329	both	326:329	This work evaluated the effectiveness of AFEX™(1) pretreatment for converting corn stover to fermentable sugars, both with and without pre-soaking in hydrogen peroxide.
24412921	8	11	theme	bioethanol	1113:1122	arg1	production					1124:1133	bioethanol production	1113:1133	bioethanol production	1113:1133	The results of the present work show that H-AFEX is a feasible pretreatment to improve the enzymatic saccharification of corn stover for bioethanol production.
24412921	8	12	theme	present	995:1001	arg1	work					1003:1006	the present work	991:1006	the present work	991:1006	The results of the present work show that H-AFEX is a feasible pretreatment to improve the enzymatic saccharification of corn stover for bioethanol production.
24412921	5	13	theme	H-AFEX	636:641	arg1	process					643:649	the H-AFEX process	632:649	the H-AFEX process	632:649	Compared with AFEX pretreatment, the H-AFEX process enhanced delignification and enzymatic hydrolysis yields of both glucose and xylose.
24412921	2	14	theme	pretreatment	263:274	arg1	effectiveness					237:249	the effectiveness	233:249	the effectiveness of AFEX™(1) pretreatment for converting corn stover to fermentable sugars, both with and without pre-soaking in hydrogen peroxide	233:379	This work evaluated the effectiveness of AFEX™(1) pretreatment for converting corn stover to fermentable sugars, both with and without pre-soaking in hydrogen peroxide.
24412921	6	15	theme	maximum	740:746	arg1	%					807:807	87.78%	802:807	87.78%	802:807	The maximum glucan and xylan digestibility of H-AFEX process were 87.78% and 90.64%, respectively, and were obtained using 0.7 (w/w) water loading, 1.0 (w/w) ammonia loading, 0.5 (w/w) 30wt.
24412921	6	15	theme	maximum	740:746	arg1	glucan					748:753	The maximum glucan and xylan digestibility	736:777	glucan	748:753	The maximum glucan and xylan digestibility of H-AFEX process were 87.78% and 90.64%, respectively, and were obtained using 0.7 (w/w) water loading, 1.0 (w/w) ammonia loading, 0.5 (w/w) 30wt.
24412921	3	16	theme	AFEX-treated	439:450	arg1	biomass					471:477	AFEX-treated and H-AFEX-treated biomass	439:477	AFEX-treated and H-AFEX-treated biomass	439:477	The compositional changes and enzymatic digestibility of AFEX-treated and H-AFEX-treated biomass were investigated.
24412921	5	17	theme	delignification	660:674	arg1	yields					701:706	delignification and enzymatic hydrolysis yields	660:706	delignification and enzymatic hydrolysis yields of both glucose and xylose	660:733	Compared with AFEX pretreatment, the H-AFEX process enhanced delignification and enzymatic hydrolysis yields of both glucose and xylose.
24412921	8	18	theme	enzymatic	1067:1075	arg1	saccharification					1077:1092	the enzymatic saccharification	1063:1092	the enzymatic saccharification of corn stover for bioethanol production	1063:1133	The results of the present work show that H-AFEX is a feasible pretreatment to improve the enzymatic saccharification of corn stover for bioethanol production.
24412921	2	19	theme	fermentable	306:316	arg1	sugars					318:323	fermentable sugars	306:323	fermentable sugars	306:323	This work evaluated the effectiveness of AFEX™(1) pretreatment for converting corn stover to fermentable sugars, both with and without pre-soaking in hydrogen peroxide.
24412921	8	20	theme	feasible	1030:1037	arg1	pretreatment					1039:1050	a feasible pretreatment to improve the enzymatic saccharification of corn stover for bioethanol production	1028:1133	a feasible pretreatment to improve the enzymatic saccharification of corn stover for bioethanol production	1028:1133	The results of the present work show that H-AFEX is a feasible pretreatment to improve the enzymatic saccharification of corn stover for bioethanol production.
24412921	8	20	theme	feasible	1030:1037	arg1	H-AFEX					1018:1023	H-AFEX	1018:1023	H-AFEX	1018:1023	The results of the present work show that H-AFEX is a feasible pretreatment to improve the enzymatic saccharification of corn stover for bioethanol production.
24412921	8	21	theme	work	1003:1006	arg1	results					980:986	The results	976:986	The results of the present work	976:1006	The results of the present work show that H-AFEX is a feasible pretreatment to improve the enzymatic saccharification of corn stover for bioethanol production.
24412921	2	22	theme	hydrogen	363:370	arg1	peroxide					372:379	hydrogen peroxide	363:379	hydrogen peroxide	363:379	This work evaluated the effectiveness of AFEX™(1) pretreatment for converting corn stover to fermentable sugars, both with and without pre-soaking in hydrogen peroxide.
24412921	0	23	theme	changes	25:31	arg1	Effects					0:6	Effects	0:6	Effects of compositional changes of AFEX-treated and H-AFEX-treated corn stover on enzymatic digestibility	0:105	Effects of compositional changes of AFEX-treated and H-AFEX-treated corn stover on enzymatic digestibility.
24412921	2	24	from	pre-soaking	348:358	arg1	peroxide					372:379	hydrogen peroxide	363:379	hydrogen peroxide	363:379	This work evaluated the effectiveness of AFEX™(1) pretreatment for converting corn stover to fermentable sugars, both with and without pre-soaking in hydrogen peroxide.
24412921	1	25	theme	Corn	108:111	arg1	stover					113:118	Corn stover	108:118	Corn stover	108:118	Corn stover is one of the main agricultural residues being considered as a cellulosic ethanol feedstock.
24412921	7	26	dep	peroxide	937:944	arg1	loading					946:952	loading	946:952	loading	946:952	% hydrogen peroxide loading, and 130°C for 10min.
24412921	1	27	theme	ethanol	194:200	arg1	feedstock					202:210	a cellulosic ethanol feedstock	181:210	a cellulosic ethanol feedstock	181:210	Corn stover is one of the main agricultural residues being considered as a cellulosic ethanol feedstock.
24412921	0	28	theme	compositional	11:23	arg1	changes					25:31	compositional changes	11:31	compositional changes of AFEX-treated and H-AFEX-treated corn stover	11:78	Effects of compositional changes of AFEX-treated and H-AFEX-treated corn stover on enzymatic digestibility.
24412921	6	29	theme	water	869:873	arg1	loading					875:881	0.7 (w/w) water loading	859:881	0.7 (w/w) water loading	859:881	The maximum glucan and xylan digestibility of H-AFEX process were 87.78% and 90.64%, respectively, and were obtained using 0.7 (w/w) water loading, 1.0 (w/w) ammonia loading, 0.5 (w/w) 30wt.
24412921	6	29	theme	water	869:873	arg1	loading					902:908	1.0 (w/w) ammonia loading	884:908	1.0 (w/w) ammonia loading	884:908	The maximum glucan and xylan digestibility of H-AFEX process were 87.78% and 90.64%, respectively, and were obtained using 0.7 (w/w) water loading, 1.0 (w/w) ammonia loading, 0.5 (w/w) 30wt.
24412921	2	30	theme	corn	291:294	arg1	stover					296:301	corn stover	291:301	corn stover	291:301	This work evaluated the effectiveness of AFEX™(1) pretreatment for converting corn stover to fermentable sugars, both with and without pre-soaking in hydrogen peroxide.
24412921	2	31	dep	pretreatment	263:274	arg1	1					260:260	1	260:260	1	260:260	This work evaluated the effectiveness of AFEX™(1) pretreatment for converting corn stover to fermentable sugars, both with and without pre-soaking in hydrogen peroxide.
24412921	0	32	theme	AFEX-treated	36:47	arg1	stover					73:78	AFEX-treated and H-AFEX-treated corn stover	36:78	AFEX-treated and H-AFEX-treated corn stover	36:78	Effects of compositional changes of AFEX-treated and H-AFEX-treated corn stover on enzymatic digestibility.
24412921	3	33	theme	H-AFEX-treated	456:469	arg1	biomass					471:477	AFEX-treated and H-AFEX-treated biomass	439:477	AFEX-treated and H-AFEX-treated biomass	439:477	The compositional changes and enzymatic digestibility of AFEX-treated and H-AFEX-treated biomass were investigated.
24412921	1	34	theme	cellulosic	183:192	arg1	feedstock					202:210	a cellulosic ethanol feedstock	181:210	a cellulosic ethanol feedstock	181:210	Corn stover is one of the main agricultural residues being considered as a cellulosic ethanol feedstock.
24412921	2	35	dep	effectiveness	237:249	arg1	converting					280:289	converting	280:289	converting corn stover to fermentable sugars	280:323	This work evaluated the effectiveness of AFEX™(1) pretreatment for converting corn stover to fermentable sugars, both with and without pre-soaking in hydrogen peroxide.
24412921	3	36	theme	enzymatic	412:420	arg1	digestibility					422:434	enzymatic digestibility	412:434	enzymatic digestibility	412:434	The compositional changes and enzymatic digestibility of AFEX-treated and H-AFEX-treated biomass were investigated.
24412921	3	37	theme	biomass	471:477	arg1	changes					400:406	The compositional changes	382:406	The compositional changes	382:406	The compositional changes and enzymatic digestibility of AFEX-treated and H-AFEX-treated biomass were investigated.
24412921	3	37	theme	biomass	471:477	arg1	digestibility					422:434	enzymatic digestibility	412:434	enzymatic digestibility	412:434	The compositional changes and enzymatic digestibility of AFEX-treated and H-AFEX-treated biomass were investigated.
24412921	0	38	theme	H-AFEX-treated	53:66	arg1	stover					73:78	AFEX-treated and H-AFEX-treated corn stover	36:78	AFEX-treated and H-AFEX-treated corn stover	36:78	Effects of compositional changes of AFEX-treated and H-AFEX-treated corn stover on enzymatic digestibility.
24412921	5	39	theme	glucose	716:722	arg1	yields					701:706	delignification and enzymatic hydrolysis yields	660:706	delignification and enzymatic hydrolysis yields of both glucose and xylose	660:733	Compared with AFEX pretreatment, the H-AFEX process enhanced delignification and enzymatic hydrolysis yields of both glucose and xylose.
24412921	6	40	theme	process	789:795	arg1	digestibility					765:777	The maximum glucan and xylan digestibility	736:777	digestibility	765:777	The maximum glucan and xylan digestibility of H-AFEX process were 87.78% and 90.64%, respectively, and were obtained using 0.7 (w/w) water loading, 1.0 (w/w) ammonia loading, 0.5 (w/w) 30wt.
24412921	6	40	theme	process	789:795	arg1	%					807:807	87.78%	802:807	87.78%	802:807	The maximum glucan and xylan digestibility of H-AFEX process were 87.78% and 90.64%, respectively, and were obtained using 0.7 (w/w) water loading, 1.0 (w/w) ammonia loading, 0.5 (w/w) 30wt.
24412921	6	40	theme	process	789:795	arg1	glucan					748:753	The maximum glucan and xylan digestibility	736:777	glucan	748:753	The maximum glucan and xylan digestibility of H-AFEX process were 87.78% and 90.64%, respectively, and were obtained using 0.7 (w/w) water loading, 1.0 (w/w) ammonia loading, 0.5 (w/w) 30wt.
24412921	5	41	theme	AFEX	613:616	arg1	pretreatment					618:629	AFEX pretreatment	613:629	AFEX pretreatment	613:629	Compared with AFEX pretreatment, the H-AFEX process enhanced delignification and enzymatic hydrolysis yields of both glucose and xylose.
24412921	6	42	theme	H-AFEX	782:787	arg1	process					789:795	H-AFEX process	782:795	H-AFEX process	782:795	The maximum glucan and xylan digestibility of H-AFEX process were 87.78% and 90.64%, respectively, and were obtained using 0.7 (w/w) water loading, 1.0 (w/w) ammonia loading, 0.5 (w/w) 30wt.
24412921	7	43	theme	hydrogen	928:935	arg1	peroxide					937:944	% hydrogen peroxide loading, and 130°C	926:963	peroxide	937:944	% hydrogen peroxide loading, and 130°C for 10min.
24412921	5	44	theme	xylose	728:733	arg1	yields					701:706	delignification and enzymatic hydrolysis yields	660:706	delignification and enzymatic hydrolysis yields of both glucose and xylose	660:733	Compared with AFEX pretreatment, the H-AFEX process enhanced delignification and enzymatic hydrolysis yields of both glucose and xylose.
24412921	0	45	theme	stover	73:78	arg1	changes					25:31	compositional changes	11:31	compositional changes of AFEX-treated and H-AFEX-treated corn stover	11:78	Effects of compositional changes of AFEX-treated and H-AFEX-treated corn stover on enzymatic digestibility.
24412921	2	46	theme	AFEX™	254:258	arg1	pretreatment					263:274	AFEX™(1) pretreatment	254:274	AFEX™(1) pretreatment	254:274	This work evaluated the effectiveness of AFEX™(1) pretreatment for converting corn stover to fermentable sugars, both with and without pre-soaking in hydrogen peroxide.
24412921	1	47	theme	main	134:137	arg1	residues					152:159	the main agricultural residues	130:159	the main agricultural residues being considered as a cellulosic ethanol feedstock	130:210	Corn stover is one of the main agricultural residues being considered as a cellulosic ethanol feedstock.
24412921	0	48	theme	corn	68:71	arg1	stover					73:78	AFEX-treated and H-AFEX-treated corn stover	36:78	AFEX-treated and H-AFEX-treated corn stover	36:78	Effects of compositional changes of AFEX-treated and H-AFEX-treated corn stover on enzymatic digestibility.
24412921	6	49	theme	xylan	759:763	arg1	digestibility					765:777	The maximum glucan and xylan digestibility	736:777	digestibility	765:777	The maximum glucan and xylan digestibility of H-AFEX process were 87.78% and 90.64%, respectively, and were obtained using 0.7 (w/w) water loading, 1.0 (w/w) ammonia loading, 0.5 (w/w) 30wt.
24412921	1	50	theme	agricultural	139:150	arg1	residues					152:159	the main agricultural residues	130:159	the main agricultural residues being considered as a cellulosic ethanol feedstock	130:210	Corn stover is one of the main agricultural residues being considered as a cellulosic ethanol feedstock.
25589358	0	0	from	analysis	22:29	arg1	matrix					75:80	the extracellular matrix	57:80	the extracellular matrix of the yeast Saccharomyces cerevisiae	57:118	Elemental biochemical analysis of the polysaccharides in the extracellular matrix of the yeast Saccharomyces cerevisiae.
25589358	6	1	theme	agarose	928:934	arg1	electrophoresis					940:954	diaminopropane agarose gel electrophoresis	913:954	diaminopropane agarose gel electrophoresis	913:954	In addition, these polysaccharide species were separated by diaminopropane agarose gel electrophoresis and induced metachromatic shift, suggesting chemical substitution, which was corroborated by anticoagulation activity.
25589358	6	2	theme	anticoagulation	1049:1063	arg1	activity					1065:1072	anticoagulation activity	1049:1072	anticoagulation activity	1049:1072	In addition, these polysaccharide species were separated by diaminopropane agarose gel electrophoresis and induced metachromatic shift, suggesting chemical substitution, which was corroborated by anticoagulation activity.
25589358	6	3	theme	metachromatic	968:980	arg1	shift					982:986	metachromatic shift	968:986	metachromatic shift	968:986	In addition, these polysaccharide species were separated by diaminopropane agarose gel electrophoresis and induced metachromatic shift, suggesting chemical substitution, which was corroborated by anticoagulation activity.
25589358	3	4	theme	gas	511:513	arg1	spectrometry					535:546	gas chromatography-mass spectrometry	511:546	gas chromatography-mass spectrometry	511:546	An analytical-grade pure glycoside fraction was obtained, fractionated by anionic exchange liquid chromatography and analyzed by gas chromatography-mass spectrometry and polyacrylamide gel electrophoresis.
25589358	4	5	theme	weight	612:617	arg1	polysaccharides					619:633	Two different molecular weight polysaccharides	588:633	Two different molecular weight polysaccharides	588:633	Two different molecular weight polysaccharides were found, composed of glucose, mannose and small relative amounts of galactose.
25589358	0	6	theme	Saccharomyces	95:107	arg1	cerevisiae					109:118	the yeast Saccharomyces cerevisiae	85:118	the yeast Saccharomyces cerevisiae	85:118	Elemental biochemical analysis of the polysaccharides in the extracellular matrix of the yeast Saccharomyces cerevisiae.
25589358	7	7	theme	Hedgehog	1173:1180	arg1	modulators					1182:1191	the mammalian Hedgehog modulators Hhatl and Hhat	1159:1206	the mammalian Hedgehog modulators Hhatl and Hhat	1159:1206	This was shown to be associated with the double deletion of the yeast homologues of the mammalian Hedgehog modulators Hhatl and Hhat, respectively yeast Gup1 and Gup2.
25589358	7	7	theme	Hedgehog	1173:1180	arg1	Hhat					1203:1206	Hhat	1203:1206	Hhat	1203:1206	This was shown to be associated with the double deletion of the yeast homologues of the mammalian Hedgehog modulators Hhatl and Hhat, respectively yeast Gup1 and Gup2.
25589358	7	7	theme	Hedgehog	1173:1180	arg1	Hhatl					1193:1197	Hhatl	1193:1197	Hhatl	1193:1197	This was shown to be associated with the double deletion of the yeast homologues of the mammalian Hedgehog modulators Hhatl and Hhat, respectively yeast Gup1 and Gup2.
25589358	7	7	theme	Hedgehog	1173:1180	arg1	Gup1					1228:1231	yeast Gup1	1222:1231	yeast Gup1	1222:1231	This was shown to be associated with the double deletion of the yeast homologues of the mammalian Hedgehog modulators Hhatl and Hhat, respectively yeast Gup1 and Gup2.
25589358	7	7	theme	Hedgehog	1173:1180	arg1	Gup2					1237:1240	Gup2	1237:1240	Gup2	1237:1240	This was shown to be associated with the double deletion of the yeast homologues of the mammalian Hedgehog modulators Hhatl and Hhat, respectively yeast Gup1 and Gup2.
25589358	7	8	dep	modulators	1182:1191	arg1	modulators					1182:1191	the mammalian Hedgehog modulators Hhatl and Hhat	1159:1206	the mammalian Hedgehog modulators Hhatl and Hhat	1159:1206	This was shown to be associated with the double deletion of the yeast homologues of the mammalian Hedgehog modulators Hhatl and Hhat, respectively yeast Gup1 and Gup2.
25589358	7	8	dep	modulators	1182:1191	arg1	Hhat					1203:1206	Hhat	1203:1206	Hhat	1203:1206	This was shown to be associated with the double deletion of the yeast homologues of the mammalian Hedgehog modulators Hhatl and Hhat, respectively yeast Gup1 and Gup2.
25589358	7	8	dep	modulators	1182:1191	arg1	Hhatl					1193:1197	Hhatl	1193:1197	Hhatl	1193:1197	This was shown to be associated with the double deletion of the yeast homologues of the mammalian Hedgehog modulators Hhatl and Hhat, respectively yeast Gup1 and Gup2.
25589358	3	9	theme	gel	567:569	arg1	electrophoresis					571:585	polyacrylamide gel electrophoresis	552:585	polyacrylamide gel electrophoresis	552:585	An analytical-grade pure glycoside fraction was obtained, fractionated by anionic exchange liquid chromatography and analyzed by gas chromatography-mass spectrometry and polyacrylamide gel electrophoresis.
25589358	0	10	theme	yeast	89:93	arg1	cerevisiae					109:118	the yeast Saccharomyces cerevisiae	85:118	the yeast Saccharomyces cerevisiae	85:118	Elemental biochemical analysis of the polysaccharides in the extracellular matrix of the yeast Saccharomyces cerevisiae.
25589358	7	11	theme	modulators	1182:1191	arg1	homologues					1145:1154	the yeast homologues	1135:1154	the yeast homologues of the mammalian Hedgehog modulators Hhatl and Hhat, respectively yeast Gup1 and Gup2	1135:1240	This was shown to be associated with the double deletion of the yeast homologues of the mammalian Hedgehog modulators Hhatl and Hhat, respectively yeast Gup1 and Gup2.
25589358	1	12	theme	glycosidic	249:258	arg1	nature					260:265	glycosidic nature	249:265	glycosidic nature	249:265	In yeast multicellular aggregates, such as biofilms and colonies, cells are supported by a yeast extracellular matrix (yECM) of glycosidic nature, the composition of which is mostly unknown.
25589358	8	13	dep	S.	1322:1323	arg1	cerevisiae					1325:1334	cerevisiae	1325:1334	cerevisiae	1325:1334	These results pioneer the study of the molecular biology of the ECM supporting S. cerevisiae multicellular aggregates such as biofilms.
25589358	8	14	theme	ECM	1307:1309	arg1	biology					1292:1298	the molecular biology	1278:1298	the molecular biology of the ECM supporting S. cerevisiae multicellular aggregates such as biofilms	1278:1376	These results pioneer the study of the molecular biology of the ECM supporting S. cerevisiae multicellular aggregates such as biofilms.
25589358	0	15	theme	cerevisiae	109:118	arg1	matrix					75:80	the extracellular matrix	57:80	the extracellular matrix of the yeast Saccharomyces cerevisiae	57:118	Elemental biochemical analysis of the polysaccharides in the extracellular matrix of the yeast Saccharomyces cerevisiae.
25589358	0	16	from	polysaccharides	38:52	arg1	matrix					75:80	the extracellular matrix	57:80	the extracellular matrix of the yeast Saccharomyces cerevisiae	57:118	Elemental biochemical analysis of the polysaccharides in the extracellular matrix of the yeast Saccharomyces cerevisiae.
25589358	3	17	theme	pure	402:405	arg1	fraction					417:424	An analytical-grade pure glycoside fraction	382:424	An analytical-grade pure glycoside fraction	382:424	An analytical-grade pure glycoside fraction was obtained, fractionated by anionic exchange liquid chromatography and analyzed by gas chromatography-mass spectrometry and polyacrylamide gel electrophoresis.
25589358	3	18	theme	polyacrylamide	552:565	arg1	electrophoresis					571:585	polyacrylamide gel electrophoresis	552:585	polyacrylamide gel electrophoresis	552:585	An analytical-grade pure glycoside fraction was obtained, fractionated by anionic exchange liquid chromatography and analyzed by gas chromatography-mass spectrometry and polyacrylamide gel electrophoresis.
25589358	4	19	theme	relative	686:693	arg1	amounts					695:701	small relative amounts	680:701	small relative amounts of galactose	680:714	Two different molecular weight polysaccharides were found, composed of glucose, mannose and small relative amounts of galactose.
25589358	4	19	theme	relative	686:693	arg1	galactose					706:714	galactose	706:714	galactose	706:714	Two different molecular weight polysaccharides were found, composed of glucose, mannose and small relative amounts of galactose.
25589358	0	20	theme	biochemical	10:20	arg1	analysis					22:29	Elemental biochemical analysis	0:29	Elemental biochemical analysis of the polysaccharides in the extracellular matrix of the yeast Saccharomyces cerevisiae	0:118	Elemental biochemical analysis of the polysaccharides in the extracellular matrix of the yeast Saccharomyces cerevisiae.
25589358	4	21	theme	different	592:600	arg1	polysaccharides					619:633	Two different molecular weight polysaccharides	588:633	Two different molecular weight polysaccharides	588:633	Two different molecular weight polysaccharides were found, composed of glucose, mannose and small relative amounts of galactose.
25589358	7	22	theme	homologues	1145:1154	arg1	deletion					1123:1130	the double deletion	1112:1130	the double deletion of the yeast homologues of the mammalian Hedgehog modulators Hhatl and Hhat, respectively yeast Gup1 and Gup2	1112:1240	This was shown to be associated with the double deletion of the yeast homologues of the mammalian Hedgehog modulators Hhatl and Hhat, respectively yeast Gup1 and Gup2.
25589358	7	23	theme	double	1116:1121	arg1	deletion					1123:1130	the double deletion	1112:1130	the double deletion of the yeast homologues of the mammalian Hedgehog modulators Hhatl and Hhat, respectively yeast Gup1 and Gup2	1112:1240	This was shown to be associated with the double deletion of the yeast homologues of the mammalian Hedgehog modulators Hhatl and Hhat, respectively yeast Gup1 and Gup2.
25589358	4	24	theme	galactose	706:714	arg1	amounts					695:701	small relative amounts	680:701	small relative amounts of galactose	680:714	Two different molecular weight polysaccharides were found, composed of glucose, mannose and small relative amounts of galactose.
25589358	4	24	theme	galactose	706:714	arg1	galactose					706:714	galactose	706:714	galactose	706:714	Two different molecular weight polysaccharides were found, composed of glucose, mannose and small relative amounts of galactose.
25589358	4	24	theme	galactose	706:714	arg1	mannose					668:674	mannose	668:674	mannose	668:674	Two different molecular weight polysaccharides were found, composed of glucose, mannose and small relative amounts of galactose.
25589358	4	24	theme	galactose	706:714	arg1	glucose					659:665	glucose	659:665	glucose	659:665	Two different molecular weight polysaccharides were found, composed of glucose, mannose and small relative amounts of galactose.
25589358	0	25	theme	Elemental	0:8	arg1	analysis					22:29	Elemental biochemical analysis	0:29	Elemental biochemical analysis of the polysaccharides in the extracellular matrix of the yeast Saccharomyces cerevisiae	0:118	Elemental biochemical analysis of the polysaccharides in the extracellular matrix of the yeast Saccharomyces cerevisiae.
25589358	1	26	theme	nature	260:265	arg1	matrix					232:237	a yeast extracellular matrix	210:237	a yeast extracellular matrix (yECM) of glycosidic nature, the composition of which is mostly unknown	210:309	In yeast multicellular aggregates, such as biofilms and colonies, cells are supported by a yeast extracellular matrix (yECM) of glycosidic nature, the composition of which is mostly unknown.
25589358	1	26	theme	nature	260:265	arg1	unknown					303:309	unknown	303:309	unknown	303:309	In yeast multicellular aggregates, such as biofilms and colonies, cells are supported by a yeast extracellular matrix (yECM) of glycosidic nature, the composition of which is mostly unknown.
25589358	1	26	theme	nature	260:265	arg1	composition					272:282	the composition	268:282	the composition	268:282	In yeast multicellular aggregates, such as biofilms and colonies, cells are supported by a yeast extracellular matrix (yECM) of glycosidic nature, the composition of which is mostly unknown.
25589358	1	26	theme	nature	260:265	arg1	yECM					240:243	yECM	240:243	yECM	240:243	In yeast multicellular aggregates, such as biofilms and colonies, cells are supported by a yeast extracellular matrix (yECM) of glycosidic nature, the composition of which is mostly unknown.
25589358	5	27	contain	had	744:746	arg1	polysaccharides					728:742	the polysaccharides	724:742	the polysaccharides	724:742	One of the polysaccharides had a low molecular weight, compatible with the association with glycoproteins abundantly occurring in yECM.
25589358	5	27	contain	had	744:746	arg1	One					717:719	One	717:719	One	717:719	One of the polysaccharides had a low molecular weight, compatible with the association with glycoproteins abundantly occurring in yECM.
25589358	5	27	contain	had	744:746	arg2	weight					764:769	a low molecular weight	748:769	a low molecular weight	748:769	One of the polysaccharides had a low molecular weight, compatible with the association with glycoproteins abundantly occurring in yECM.
25589358	5	28	theme	polysaccharides	728:742	arg1	One					717:719	One	717:719	One	717:719	One of the polysaccharides had a low molecular weight, compatible with the association with glycoproteins abundantly occurring in yECM.
25589358	5	28	theme	polysaccharides	728:742	arg1	polysaccharides					728:742	the polysaccharides	724:742	the polysaccharides	724:742	One of the polysaccharides had a low molecular weight, compatible with the association with glycoproteins abundantly occurring in yECM.
25589358	3	29	theme	anionic	456:462	arg1	chromatography					480:493	anionic exchange liquid chromatography	456:493	anionic exchange liquid chromatography	456:493	An analytical-grade pure glycoside fraction was obtained, fractionated by anionic exchange liquid chromatography and analyzed by gas chromatography-mass spectrometry and polyacrylamide gel electrophoresis.
25589358	4	30	theme	molecular	602:610	arg1	polysaccharides					619:633	Two different molecular weight polysaccharides	588:633	Two different molecular weight polysaccharides	588:633	Two different molecular weight polysaccharides were found, composed of glucose, mannose and small relative amounts of galactose.
25589358	7	31	theme	yeast	1139:1143	arg1	homologues					1145:1154	the yeast homologues	1135:1154	the yeast homologues of the mammalian Hedgehog modulators Hhatl and Hhat, respectively yeast Gup1 and Gup2	1135:1240	This was shown to be associated with the double deletion of the yeast homologues of the mammalian Hedgehog modulators Hhatl and Hhat, respectively yeast Gup1 and Gup2.
25589358	4	32	dep	found	640:644	arg1	composed					647:654	composed	647:654	composed of glucose, mannose and small relative amounts of galactose	647:714	Two different molecular weight polysaccharides were found, composed of glucose, mannose and small relative amounts of galactose.
25589358	2	33	dep	Saccharomyces	312:324	arg1	cerevisiae					326:335	cerevisiae	326:335	cerevisiae	326:335	Saccharomyces cerevisiae ECM was produced, extracted and partitioned.
25589358	7	34	theme	yeast	1222:1226	arg1	modulators					1182:1191	the mammalian Hedgehog modulators Hhatl and Hhat	1159:1206	the mammalian Hedgehog modulators Hhatl and Hhat	1159:1206	This was shown to be associated with the double deletion of the yeast homologues of the mammalian Hedgehog modulators Hhatl and Hhat, respectively yeast Gup1 and Gup2.
25589358	7	34	theme	yeast	1222:1226	arg1	Gup1					1228:1231	yeast Gup1	1222:1231	yeast Gup1	1222:1231	This was shown to be associated with the double deletion of the yeast homologues of the mammalian Hedgehog modulators Hhatl and Hhat, respectively yeast Gup1 and Gup2.
25589358	3	35	theme	exchange	464:471	arg1	chromatography					480:493	anionic exchange liquid chromatography	456:493	anionic exchange liquid chromatography	456:493	An analytical-grade pure glycoside fraction was obtained, fractionated by anionic exchange liquid chromatography and analyzed by gas chromatography-mass spectrometry and polyacrylamide gel electrophoresis.
25589358	0	36	theme	polysaccharides	38:52	arg1	analysis					22:29	Elemental biochemical analysis	0:29	Elemental biochemical analysis of the polysaccharides in the extracellular matrix of the yeast Saccharomyces cerevisiae	0:118	Elemental biochemical analysis of the polysaccharides in the extracellular matrix of the yeast Saccharomyces cerevisiae.
25589358	2	37	theme	Saccharomyces	312:324	arg1	ECM					337:339	Saccharomyces cerevisiae ECM	312:339	Saccharomyces cerevisiae ECM	312:339	Saccharomyces cerevisiae ECM was produced, extracted and partitioned.
25589358	8	38	theme	multicellular	1336:1348	arg1	aggregates					1350:1359	S. cerevisiae multicellular aggregates	1322:1359	S. cerevisiae multicellular aggregates such as biofilms	1322:1376	These results pioneer the study of the molecular biology of the ECM supporting S. cerevisiae multicellular aggregates such as biofilms.
25589358	8	38	theme	multicellular	1336:1348	arg1	biofilms					1369:1376	biofilms	1369:1376	biofilms	1369:1376	These results pioneer the study of the molecular biology of the ECM supporting S. cerevisiae multicellular aggregates such as biofilms.
25589358	4	39	theme	small	680:684	arg1	amounts					695:701	small relative amounts	680:701	small relative amounts of galactose	680:714	Two different molecular weight polysaccharides were found, composed of glucose, mannose and small relative amounts of galactose.
25589358	4	39	theme	small	680:684	arg1	galactose					706:714	galactose	706:714	galactose	706:714	Two different molecular weight polysaccharides were found, composed of glucose, mannose and small relative amounts of galactose.
25589358	3	40	theme	glycoside	407:415	arg1	fraction					417:424	An analytical-grade pure glycoside fraction	382:424	An analytical-grade pure glycoside fraction	382:424	An analytical-grade pure glycoside fraction was obtained, fractionated by anionic exchange liquid chromatography and analyzed by gas chromatography-mass spectrometry and polyacrylamide gel electrophoresis.
25589358	5	41	theme	low	750:752	arg1	weight					764:769	a low molecular weight	748:769	a low molecular weight	748:769	One of the polysaccharides had a low molecular weight, compatible with the association with glycoproteins abundantly occurring in yECM.
25589358	0	42	from	matrix	75:80	arg1	analysis					22:29	Elemental biochemical analysis	0:29	Elemental biochemical analysis of the polysaccharides in the extracellular matrix of the yeast Saccharomyces cerevisiae	0:118	Elemental biochemical analysis of the polysaccharides in the extracellular matrix of the yeast Saccharomyces cerevisiae.
25589358	5	43	with	compatible	772:781	arg1	association					792:802	the association	788:802	the association with glycoproteins abundantly occurring in yECM	788:850	One of the polysaccharides had a low molecular weight, compatible with the association with glycoproteins abundantly occurring in yECM.
25589358	5	44	theme	compatible	772:781	arg1	weight					764:769	a low molecular weight	748:769	a low molecular weight	748:769	One of the polysaccharides had a low molecular weight, compatible with the association with glycoproteins abundantly occurring in yECM.
25589358	8	45	theme	molecular	1282:1290	arg1	biology					1292:1298	the molecular biology	1278:1298	the molecular biology of the ECM supporting S. cerevisiae multicellular aggregates such as biofilms	1278:1376	These results pioneer the study of the molecular biology of the ECM supporting S. cerevisiae multicellular aggregates such as biofilms.
25589358	5	46	theme	molecular	754:762	arg1	weight					764:769	a low molecular weight	748:769	a low molecular weight	748:769	One of the polysaccharides had a low molecular weight, compatible with the association with glycoproteins abundantly occurring in yECM.
25589358	6	47	theme	diaminopropane	913:926	arg1	electrophoresis					940:954	diaminopropane agarose gel electrophoresis	913:954	diaminopropane agarose gel electrophoresis	913:954	In addition, these polysaccharide species were separated by diaminopropane agarose gel electrophoresis and induced metachromatic shift, suggesting chemical substitution, which was corroborated by anticoagulation activity.
25589358	6	48	theme	gel	936:938	arg1	electrophoresis					940:954	diaminopropane agarose gel electrophoresis	913:954	diaminopropane agarose gel electrophoresis	913:954	In addition, these polysaccharide species were separated by diaminopropane agarose gel electrophoresis and induced metachromatic shift, suggesting chemical substitution, which was corroborated by anticoagulation activity.
25589358	3	49	theme	analytical-grade	385:400	arg1	fraction					417:424	An analytical-grade pure glycoside fraction	382:424	An analytical-grade pure glycoside fraction	382:424	An analytical-grade pure glycoside fraction was obtained, fractionated by anionic exchange liquid chromatography and analyzed by gas chromatography-mass spectrometry and polyacrylamide gel electrophoresis.
25589358	6	50	theme	chemical	1000:1007	arg1	substitution					1009:1020	chemical substitution	1000:1020	chemical substitution	1000:1020	In addition, these polysaccharide species were separated by diaminopropane agarose gel electrophoresis and induced metachromatic shift, suggesting chemical substitution, which was corroborated by anticoagulation activity.
25589358	3	51	theme	liquid	473:478	arg1	chromatography					480:493	anionic exchange liquid chromatography	456:493	anionic exchange liquid chromatography	456:493	An analytical-grade pure glycoside fraction was obtained, fractionated by anionic exchange liquid chromatography and analyzed by gas chromatography-mass spectrometry and polyacrylamide gel electrophoresis.
25589358	7	52	theme	mammalian	1163:1171	arg1	modulators					1182:1191	the mammalian Hedgehog modulators Hhatl and Hhat	1159:1206	the mammalian Hedgehog modulators Hhatl and Hhat	1159:1206	This was shown to be associated with the double deletion of the yeast homologues of the mammalian Hedgehog modulators Hhatl and Hhat, respectively yeast Gup1 and Gup2.
25589358	7	52	theme	mammalian	1163:1171	arg1	Hhat					1203:1206	Hhat	1203:1206	Hhat	1203:1206	This was shown to be associated with the double deletion of the yeast homologues of the mammalian Hedgehog modulators Hhatl and Hhat, respectively yeast Gup1 and Gup2.
25589358	7	52	theme	mammalian	1163:1171	arg1	Hhatl					1193:1197	Hhatl	1193:1197	Hhatl	1193:1197	This was shown to be associated with the double deletion of the yeast homologues of the mammalian Hedgehog modulators Hhatl and Hhat, respectively yeast Gup1 and Gup2.
25589358	7	52	theme	mammalian	1163:1171	arg1	Gup1					1228:1231	yeast Gup1	1222:1231	yeast Gup1	1222:1231	This was shown to be associated with the double deletion of the yeast homologues of the mammalian Hedgehog modulators Hhatl and Hhat, respectively yeast Gup1 and Gup2.
25589358	7	52	theme	mammalian	1163:1171	arg1	Gup2					1237:1240	Gup2	1237:1240	Gup2	1237:1240	This was shown to be associated with the double deletion of the yeast homologues of the mammalian Hedgehog modulators Hhatl and Hhat, respectively yeast Gup1 and Gup2.
25589358	1	53	theme	yeast	124:128	arg1	aggregates					144:153	yeast multicellular aggregates	124:153	yeast multicellular aggregates	124:153	In yeast multicellular aggregates, such as biofilms and colonies, cells are supported by a yeast extracellular matrix (yECM) of glycosidic nature, the composition of which is mostly unknown.
25589358	1	53	theme	yeast	124:128	arg1	colonies					177:184	colonies	177:184	colonies	177:184	In yeast multicellular aggregates, such as biofilms and colonies, cells are supported by a yeast extracellular matrix (yECM) of glycosidic nature, the composition of which is mostly unknown.
25589358	1	53	theme	yeast	124:128	arg1	biofilms					164:171	biofilms	164:171	biofilms	164:171	In yeast multicellular aggregates, such as biofilms and colonies, cells are supported by a yeast extracellular matrix (yECM) of glycosidic nature, the composition of which is mostly unknown.
25589358	6	54	theme	polysaccharide	872:885	arg1	species					887:893	these polysaccharide species	866:893	these polysaccharide species	866:893	In addition, these polysaccharide species were separated by diaminopropane agarose gel electrophoresis and induced metachromatic shift, suggesting chemical substitution, which was corroborated by anticoagulation activity.
25589358	5	55	with	association	792:802	arg1	glycoproteins					809:821	glycoproteins	809:821	glycoproteins abundantly occurring in yECM	809:850	One of the polysaccharides had a low molecular weight, compatible with the association with glycoproteins abundantly occurring in yECM.
25589358	1	56	theme	multicellular	130:142	arg1	aggregates					144:153	yeast multicellular aggregates	124:153	yeast multicellular aggregates	124:153	In yeast multicellular aggregates, such as biofilms and colonies, cells are supported by a yeast extracellular matrix (yECM) of glycosidic nature, the composition of which is mostly unknown.
25589358	1	56	theme	multicellular	130:142	arg1	colonies					177:184	colonies	177:184	colonies	177:184	In yeast multicellular aggregates, such as biofilms and colonies, cells are supported by a yeast extracellular matrix (yECM) of glycosidic nature, the composition of which is mostly unknown.
25589358	1	56	theme	multicellular	130:142	arg1	biofilms					164:171	biofilms	164:171	biofilms	164:171	In yeast multicellular aggregates, such as biofilms and colonies, cells are supported by a yeast extracellular matrix (yECM) of glycosidic nature, the composition of which is mostly unknown.
25589358	1	57	theme	yeast	212:216	arg1	matrix					232:237	a yeast extracellular matrix	210:237	a yeast extracellular matrix (yECM) of glycosidic nature, the composition of which is mostly unknown	210:309	In yeast multicellular aggregates, such as biofilms and colonies, cells are supported by a yeast extracellular matrix (yECM) of glycosidic nature, the composition of which is mostly unknown.
25589358	1	57	theme	yeast	212:216	arg1	yECM					240:243	yECM	240:243	yECM	240:243	In yeast multicellular aggregates, such as biofilms and colonies, cells are supported by a yeast extracellular matrix (yECM) of glycosidic nature, the composition of which is mostly unknown.
25589358	5	58	gly	glycoproteins	809:821	arg1	glycoproteins					809:821	glycoproteins	809:821	glycoproteins abundantly occurring in yECM	809:850	One of the polysaccharides had a low molecular weight, compatible with the association with glycoproteins abundantly occurring in yECM.
25589358	1	59	theme	extracellular	218:230	arg1	matrix					232:237	a yeast extracellular matrix	210:237	a yeast extracellular matrix (yECM) of glycosidic nature, the composition of which is mostly unknown	210:309	In yeast multicellular aggregates, such as biofilms and colonies, cells are supported by a yeast extracellular matrix (yECM) of glycosidic nature, the composition of which is mostly unknown.
25589358	1	59	theme	extracellular	218:230	arg1	yECM					240:243	yECM	240:243	yECM	240:243	In yeast multicellular aggregates, such as biofilms and colonies, cells are supported by a yeast extracellular matrix (yECM) of glycosidic nature, the composition of which is mostly unknown.
25589358	8	60	theme	S.	1322:1323	arg1	aggregates					1350:1359	S. cerevisiae multicellular aggregates	1322:1359	S. cerevisiae multicellular aggregates such as biofilms	1322:1376	These results pioneer the study of the molecular biology of the ECM supporting S. cerevisiae multicellular aggregates such as biofilms.
25589358	8	60	theme	S.	1322:1323	arg1	biofilms					1369:1376	biofilms	1369:1376	biofilms	1369:1376	These results pioneer the study of the molecular biology of the ECM supporting S. cerevisiae multicellular aggregates such as biofilms.
25589358	3	61	theme	chromatography-mass	515:533	arg1	spectrometry					535:546	gas chromatography-mass spectrometry	511:546	gas chromatography-mass spectrometry	511:546	An analytical-grade pure glycoside fraction was obtained, fractionated by anionic exchange liquid chromatography and analyzed by gas chromatography-mass spectrometry and polyacrylamide gel electrophoresis.
25589358	8	62	theme	biology	1292:1298	arg1	study					1269:1273	the study	1265:1273	the study of the molecular biology of the ECM supporting S. cerevisiae multicellular aggregates such as biofilms	1265:1376	These results pioneer the study of the molecular biology of the ECM supporting S. cerevisiae multicellular aggregates such as biofilms.
25589358	0	63	theme	extracellular	61:73	arg1	matrix					75:80	the extracellular matrix	57:80	the extracellular matrix of the yeast Saccharomyces cerevisiae	57:118	Elemental biochemical analysis of the polysaccharides in the extracellular matrix of the yeast Saccharomyces cerevisiae.
24815400	0	0	theme	Phellinus	90:98	arg1	polysaccharides					108:122	Phellinus linteus polysaccharides	90:122	Phellinus linteus polysaccharides	90:122	Effect of extraction media on preliminary characterizations and antioxidant activities of Phellinus linteus polysaccharides.
24815400	2	1	theme	carbohydrate	429:440	arg1	contents					458:465	the highest carbohydrate and uronic acid contents	417:465	the highest carbohydrate and uronic acid contents	417:465	PL-N mainly comprised xylose and arabinose with a high molecular weight (Mw) and the highest carbohydrate and uronic acid contents.
24815400	1	2	theme	Phellinus	186:194	arg1	mycelia					204:210	Phellinus linteus mycelia	186:210	Phellinus linteus mycelia	186:210	Three partially purified polysaccharides were extracted from Phellinus linteus mycelia using hot water, 1% (NH4)2C2O4, and 1.25M NaOH/0.05% NaBH4, and the extracts were named PL-W, PL-A, and, PL-N respectively.
24815400	6	3	theme	chemical	890:897	arg1	structures					899:908	chemical structures	890:908	chemical structures	890:908	The Mws, monosaccharide compositions, and chemical structures of the polysaccharides also affected their antioxidant activities.
24815400	1	4	theme	linteus	196:202	arg1	mycelia					204:210	Phellinus linteus mycelia	186:210	Phellinus linteus mycelia	186:210	Three partially purified polysaccharides were extracted from Phellinus linteus mycelia using hot water, 1% (NH4)2C2O4, and 1.25M NaOH/0.05% NaBH4, and the extracts were named PL-W, PL-A, and, PL-N respectively.
24815400	1	5	theme	1.25M	248:252	arg1	NaBH4					265:269	1.25M NaOH/0.05% NaBH4	248:269	1.25M NaOH/0.05% NaBH4	248:269	Three partially purified polysaccharides were extracted from Phellinus linteus mycelia using hot water, 1% (NH4)2C2O4, and 1.25M NaOH/0.05% NaBH4, and the extracts were named PL-W, PL-A, and, PL-N respectively.
24815400	0	6	theme	polysaccharides	108:122	arg1	characterizations					42:58	preliminary characterizations	30:58	preliminary characterizations	30:58	Effect of extraction media on preliminary characterizations and antioxidant activities of Phellinus linteus polysaccharides.
24815400	0	6	theme	polysaccharides	108:122	arg1	activities					76:85	antioxidant activities	64:85	antioxidant activities	64:85	Effect of extraction media on preliminary characterizations and antioxidant activities of Phellinus linteus polysaccharides.
24815400	0	7	from	Effect	0:5	arg1	characterizations					42:58	preliminary characterizations	30:58	preliminary characterizations	30:58	Effect of extraction media on preliminary characterizations and antioxidant activities of Phellinus linteus polysaccharides.
24815400	0	7	from	Effect	0:5	arg1	activities					76:85	antioxidant activities	64:85	antioxidant activities	64:85	Effect of extraction media on preliminary characterizations and antioxidant activities of Phellinus linteus polysaccharides.
24815400	4	8	theme	concentration-dependent	709:731	arg1	manner					733:738	a concentration-dependent manner	707:738	a concentration-dependent manner	707:738	All three polysaccharides existed as compact coils in aqueous solutions and exhibited strong scavenging capacity and antioxidant activities in a concentration-dependent manner.
24815400	1	9	theme	NaOH/0.05	254:262	arg1	NaBH4					265:269	1.25M NaOH/0.05% NaBH4	248:269	1.25M NaOH/0.05% NaBH4	248:269	Three partially purified polysaccharides were extracted from Phellinus linteus mycelia using hot water, 1% (NH4)2C2O4, and 1.25M NaOH/0.05% NaBH4, and the extracts were named PL-W, PL-A, and, PL-N respectively.
24815400	0	10	theme	linteus	100:106	arg1	polysaccharides					108:122	Phellinus linteus polysaccharides	90:122	Phellinus linteus polysaccharides	90:122	Effect of extraction media on preliminary characterizations and antioxidant activities of Phellinus linteus polysaccharides.
24815400	7	11	theme	food	1113:1116	arg1	additives					1118:1126	food additives	1113:1126	food additives	1113:1126	PL-A and PL-N had better antioxidant activities and could thus be developed as potential natural antioxidant agents for applications in food additives and biomedical industries.
24815400	3	12	from	fractions	535:543	arg1	ratios					556:561	various ratios	548:561	various ratios	548:561	PL-W and PL-A were mainly composed of glucose with high and low Mw fractions in various ratios.
24815400	1	13	theme	%	263:263	arg1	NaBH4					265:269	1.25M NaOH/0.05% NaBH4	248:269	1.25M NaOH/0.05% NaBH4	248:269	Three partially purified polysaccharides were extracted from Phellinus linteus mycelia using hot water, 1% (NH4)2C2O4, and 1.25M NaOH/0.05% NaBH4, and the extracts were named PL-W, PL-A, and, PL-N respectively.
24815400	4	14	from	coils	609:613	arg1	solutions					626:634	aqueous solutions	618:634	aqueous solutions	618:634	All three polysaccharides existed as compact coils in aqueous solutions and exhibited strong scavenging capacity and antioxidant activities in a concentration-dependent manner.
24815400	5	15	theme	antioxidant	824:834	arg1	activities					836:845	strong antioxidant activities	817:845	strong antioxidant activities	817:845	The polysaccharides also had high uronic acid and carbohydrate contents and strong antioxidant activities.
24815400	2	16	theme	acid	453:456	arg1	contents					458:465	the highest carbohydrate and uronic acid contents	417:465	the highest carbohydrate and uronic acid contents	417:465	PL-N mainly comprised xylose and arabinose with a high molecular weight (Mw) and the highest carbohydrate and uronic acid contents.
24815400	5	17	theme	high	770:773	arg1	acid					782:785	high uronic acid and carbohydrate contents	770:811	acid	782:785	The polysaccharides also had high uronic acid and carbohydrate contents and strong antioxidant activities.
24815400	7	18	theme	antioxidant	1074:1084	arg1	agents					1086:1091	potential natural antioxidant agents	1056:1091	potential natural antioxidant agents for applications in food additives and biomedical industries	1056:1152	PL-A and PL-N had better antioxidant activities and could thus be developed as potential natural antioxidant agents for applications in food additives and biomedical industries.
24815400	7	18	theme	antioxidant	1074:1084	arg1	PL-A					977:980	PL-A	977:980	PL-A	977:980	PL-A and PL-N had better antioxidant activities and could thus be developed as potential natural antioxidant agents for applications in food additives and biomedical industries.
24815400	4	19	theme	antioxidant	681:691	arg1	activities					693:702	antioxidant activities	681:702	antioxidant activities	681:702	All three polysaccharides existed as compact coils in aqueous solutions and exhibited strong scavenging capacity and antioxidant activities in a concentration-dependent manner.
24815400	4	19	theme	antioxidant	681:691	arg1	capacity					668:675	strong scavenging capacity	650:675	strong scavenging capacity	650:675	All three polysaccharides existed as compact coils in aqueous solutions and exhibited strong scavenging capacity and antioxidant activities in a concentration-dependent manner.
24815400	4	19	theme	antioxidant	681:691	arg1	coils					609:613	compact coils	601:613	compact coils in aqueous solutions	601:634	All three polysaccharides existed as compact coils in aqueous solutions and exhibited strong scavenging capacity and antioxidant activities in a concentration-dependent manner.
24815400	6	20	theme	monosaccharide	857:870	arg1	compositions					872:883	monosaccharide compositions	857:883	monosaccharide compositions	857:883	The Mws, monosaccharide compositions, and chemical structures of the polysaccharides also affected their antioxidant activities.
24815400	1	21	theme	hot	218:220	arg1	water					222:226	hot water	218:226	hot water	218:226	Three partially purified polysaccharides were extracted from Phellinus linteus mycelia using hot water, 1% (NH4)2C2O4, and 1.25M NaOH/0.05% NaBH4, and the extracts were named PL-W, PL-A, and, PL-N respectively.
24815400	5	22	theme	uronic	775:780	arg1	acid					782:785	high uronic acid and carbohydrate contents	770:811	acid	782:785	The polysaccharides also had high uronic acid and carbohydrate contents and strong antioxidant activities.
24815400	4	23	theme	compact	601:607	arg1	activities					693:702	antioxidant activities	681:702	antioxidant activities	681:702	All three polysaccharides existed as compact coils in aqueous solutions and exhibited strong scavenging capacity and antioxidant activities in a concentration-dependent manner.
24815400	4	23	theme	compact	601:607	arg1	capacity					668:675	strong scavenging capacity	650:675	strong scavenging capacity	650:675	All three polysaccharides existed as compact coils in aqueous solutions and exhibited strong scavenging capacity and antioxidant activities in a concentration-dependent manner.
24815400	4	23	theme	compact	601:607	arg1	coils					609:613	compact coils	601:613	compact coils in aqueous solutions	601:634	All three polysaccharides existed as compact coils in aqueous solutions and exhibited strong scavenging capacity and antioxidant activities in a concentration-dependent manner.
24815400	2	24	theme	molecular	391:399	arg1	weight					401:406	a high molecular weight	384:406	a high molecular weight (Mw)	384:411	PL-N mainly comprised xylose and arabinose with a high molecular weight (Mw) and the highest carbohydrate and uronic acid contents.
24815400	2	24	theme	molecular	391:399	arg1	Mw					409:410	Mw	409:410	Mw	409:410	PL-N mainly comprised xylose and arabinose with a high molecular weight (Mw) and the highest carbohydrate and uronic acid contents.
24815400	6	25	theme	antioxidant	953:963	arg1	activities					965:974	their antioxidant activities	947:974	their antioxidant activities	947:974	The Mws, monosaccharide compositions, and chemical structures of the polysaccharides also affected their antioxidant activities.
24815400	7	26	theme	potential	1056:1064	arg1	agents					1086:1091	potential natural antioxidant agents	1056:1091	potential natural antioxidant agents for applications in food additives and biomedical industries	1056:1152	PL-A and PL-N had better antioxidant activities and could thus be developed as potential natural antioxidant agents for applications in food additives and biomedical industries.
24815400	7	26	theme	potential	1056:1064	arg1	PL-A					977:980	PL-A	977:980	PL-A	977:980	PL-A and PL-N had better antioxidant activities and could thus be developed as potential natural antioxidant agents for applications in food additives and biomedical industries.
24815400	5	27	theme	carbohydrate	791:802	arg1	contents					804:811	high uronic acid and carbohydrate contents	770:811	contents	804:811	The polysaccharides also had high uronic acid and carbohydrate contents and strong antioxidant activities.
24815400	2	28	theme	high	386:389	arg1	weight					401:406	a high molecular weight	384:406	a high molecular weight (Mw)	384:411	PL-N mainly comprised xylose and arabinose with a high molecular weight (Mw) and the highest carbohydrate and uronic acid contents.
24815400	2	28	theme	high	386:389	arg1	Mw					409:410	Mw	409:410	Mw	409:410	PL-N mainly comprised xylose and arabinose with a high molecular weight (Mw) and the highest carbohydrate and uronic acid contents.
24815400	7	29	theme	natural	1066:1072	arg1	agents					1086:1091	potential natural antioxidant agents	1056:1091	potential natural antioxidant agents for applications in food additives and biomedical industries	1056:1152	PL-A and PL-N had better antioxidant activities and could thus be developed as potential natural antioxidant agents for applications in food additives and biomedical industries.
24815400	7	29	theme	natural	1066:1072	arg1	PL-A					977:980	PL-A	977:980	PL-A	977:980	PL-A and PL-N had better antioxidant activities and could thus be developed as potential natural antioxidant agents for applications in food additives and biomedical industries.
24815400	1	30	theme	1	229:229	arg1	%					230:230	%	230:230	%	230:230	Three partially purified polysaccharides were extracted from Phellinus linteus mycelia using hot water, 1% (NH4)2C2O4, and 1.25M NaOH/0.05% NaBH4, and the extracts were named PL-W, PL-A, and, PL-N respectively.
24815400	0	31	theme	preliminary	30:40	arg1	characterizations					42:58	preliminary characterizations	30:58	preliminary characterizations	30:58	Effect of extraction media on preliminary characterizations and antioxidant activities of Phellinus linteus polysaccharides.
24815400	7	32	from	applications	1097:1108	arg1	additives					1118:1126	food additives	1113:1126	food additives	1113:1126	PL-A and PL-N had better antioxidant activities and could thus be developed as potential natural antioxidant agents for applications in food additives and biomedical industries.
24815400	7	32	from	applications	1097:1108	arg1	industries					1143:1152	biomedical industries	1132:1152	biomedical industries	1132:1152	PL-A and PL-N had better antioxidant activities and could thus be developed as potential natural antioxidant agents for applications in food additives and biomedical industries.
24815400	3	33	theme	low	528:530	arg1	fractions					535:543	high and low Mw fractions	519:543	high and low Mw fractions in various ratios	519:561	PL-W and PL-A were mainly composed of glucose with high and low Mw fractions in various ratios.
24815400	1	34	theme	%	230:230	arg1	2C2O4					237:241	1% (NH4)2C2O4	229:241	1% (NH4)2C2O4	229:241	Three partially purified polysaccharides were extracted from Phellinus linteus mycelia using hot water, 1% (NH4)2C2O4, and 1.25M NaOH/0.05% NaBH4, and the extracts were named PL-W, PL-A, and, PL-N respectively.
24815400	0	35	theme	media	21:25	arg1	Effect					0:5	Effect	0:5	Effect of extraction media on preliminary characterizations and antioxidant activities of Phellinus linteus polysaccharides.	0:123	Effect of extraction media on preliminary characterizations and antioxidant activities of Phellinus linteus polysaccharides.
24815400	7	36	theme	antioxidant	1002:1012	arg1	activities					1014:1023	better antioxidant activities	995:1023	better antioxidant activities	995:1023	PL-A and PL-N had better antioxidant activities and could thus be developed as potential natural antioxidant agents for applications in food additives and biomedical industries.
24815400	5	37	theme	strong	817:822	arg1	activities					836:845	strong antioxidant activities	817:845	strong antioxidant activities	817:845	The polysaccharides also had high uronic acid and carbohydrate contents and strong antioxidant activities.
24815400	7	38	theme	biomedical	1132:1141	arg1	industries					1143:1152	biomedical industries	1132:1152	biomedical industries	1132:1152	PL-A and PL-N had better antioxidant activities and could thus be developed as potential natural antioxidant agents for applications in food additives and biomedical industries.
24815400	6	39	theme	polysaccharides	917:931	arg1	Mws					852:854	The Mws	848:854	The Mws	848:854	The Mws, monosaccharide compositions, and chemical structures of the polysaccharides also affected their antioxidant activities.
24815400	6	39	theme	polysaccharides	917:931	arg1	compositions					872:883	monosaccharide compositions	857:883	monosaccharide compositions	857:883	The Mws, monosaccharide compositions, and chemical structures of the polysaccharides also affected their antioxidant activities.
24815400	6	39	theme	polysaccharides	917:931	arg1	structures					899:908	chemical structures	890:908	chemical structures	890:908	The Mws, monosaccharide compositions, and chemical structures of the polysaccharides also affected their antioxidant activities.
24815400	2	40	dep	carbohydrate	429:440	arg1	highest					421:427	highest	421:427	highest	421:427	PL-N mainly comprised xylose and arabinose with a high molecular weight (Mw) and the highest carbohydrate and uronic acid contents.
24815400	3	41	theme	high	519:522	arg1	fractions					535:543	high and low Mw fractions	519:543	high and low Mw fractions in various ratios	519:561	PL-W and PL-A were mainly composed of glucose with high and low Mw fractions in various ratios.
24815400	4	42	theme	strong	650:655	arg1	activities					693:702	antioxidant activities	681:702	antioxidant activities	681:702	All three polysaccharides existed as compact coils in aqueous solutions and exhibited strong scavenging capacity and antioxidant activities in a concentration-dependent manner.
24815400	4	42	theme	strong	650:655	arg1	coils					609:613	compact coils	601:613	compact coils in aqueous solutions	601:634	All three polysaccharides existed as compact coils in aqueous solutions and exhibited strong scavenging capacity and antioxidant activities in a concentration-dependent manner.
24815400	4	42	theme	strong	650:655	arg1	capacity					668:675	strong scavenging capacity	650:675	strong scavenging capacity	650:675	All three polysaccharides existed as compact coils in aqueous solutions and exhibited strong scavenging capacity and antioxidant activities in a concentration-dependent manner.
24815400	5	43	contain	had	766:768	arg2	activities					836:845	strong antioxidant activities	817:845	strong antioxidant activities	817:845	The polysaccharides also had high uronic acid and carbohydrate contents and strong antioxidant activities.
24815400	5	43	contain	had	766:768	arg2	acid					782:785	high uronic acid and carbohydrate contents	770:811	acid	782:785	The polysaccharides also had high uronic acid and carbohydrate contents and strong antioxidant activities.
24815400	5	43	contain	had	766:768	arg1	polysaccharides					745:759	The polysaccharides	741:759	The polysaccharides	741:759	The polysaccharides also had high uronic acid and carbohydrate contents and strong antioxidant activities.
24815400	5	43	contain	had	766:768	arg2	contents					804:811	high uronic acid and carbohydrate contents	770:811	contents	804:811	The polysaccharides also had high uronic acid and carbohydrate contents and strong antioxidant activities.
24815400	1	44	theme	purified	141:148	arg1	polysaccharides					150:164	Three partially purified polysaccharides	125:164	Three partially purified polysaccharides	125:164	Three partially purified polysaccharides were extracted from Phellinus linteus mycelia using hot water, 1% (NH4)2C2O4, and 1.25M NaOH/0.05% NaBH4, and the extracts were named PL-W, PL-A, and, PL-N respectively.
24815400	3	45	theme	various	548:554	arg1	ratios					556:561	various ratios	548:561	various ratios	548:561	PL-W and PL-A were mainly composed of glucose with high and low Mw fractions in various ratios.
24815400	1	46	dep	%	230:230	arg1	NH4					233:235	NH4	233:235	NH4	233:235	Three partially purified polysaccharides were extracted from Phellinus linteus mycelia using hot water, 1% (NH4)2C2O4, and 1.25M NaOH/0.05% NaBH4, and the extracts were named PL-W, PL-A, and, PL-N respectively.
24815400	3	47	theme	Mw	532:533	arg1	fractions					535:543	high and low Mw fractions	519:543	high and low Mw fractions in various ratios	519:561	PL-W and PL-A were mainly composed of glucose with high and low Mw fractions in various ratios.
24815400	7	48	theme	better	995:1000	arg1	activities					1014:1023	better antioxidant activities	995:1023	better antioxidant activities	995:1023	PL-A and PL-N had better antioxidant activities and could thus be developed as potential natural antioxidant agents for applications in food additives and biomedical industries.
24815400	4	49	theme	scavenging	657:666	arg1	activities					693:702	antioxidant activities	681:702	antioxidant activities	681:702	All three polysaccharides existed as compact coils in aqueous solutions and exhibited strong scavenging capacity and antioxidant activities in a concentration-dependent manner.
24815400	4	49	theme	scavenging	657:666	arg1	coils					609:613	compact coils	601:613	compact coils in aqueous solutions	601:634	All three polysaccharides existed as compact coils in aqueous solutions and exhibited strong scavenging capacity and antioxidant activities in a concentration-dependent manner.
24815400	4	49	theme	scavenging	657:666	arg1	capacity					668:675	strong scavenging capacity	650:675	strong scavenging capacity	650:675	All three polysaccharides existed as compact coils in aqueous solutions and exhibited strong scavenging capacity and antioxidant activities in a concentration-dependent manner.
24815400	2	50	theme	uronic	446:451	arg1	contents					458:465	the highest carbohydrate and uronic acid contents	417:465	the highest carbohydrate and uronic acid contents	417:465	PL-N mainly comprised xylose and arabinose with a high molecular weight (Mw) and the highest carbohydrate and uronic acid contents.
24815400	4	51	theme	aqueous	618:624	arg1	solutions					626:634	aqueous solutions	618:634	aqueous solutions	618:634	All three polysaccharides existed as compact coils in aqueous solutions and exhibited strong scavenging capacity and antioxidant activities in a concentration-dependent manner.
24815400	0	52	theme	antioxidant	64:74	arg1	activities					76:85	antioxidant activities	64:85	antioxidant activities	64:85	Effect of extraction media on preliminary characterizations and antioxidant activities of Phellinus linteus polysaccharides.
24815400	0	53	theme	extraction	10:19	arg1	media					21:25	extraction media	10:25	extraction media	10:25	Effect of extraction media on preliminary characterizations and antioxidant activities of Phellinus linteus polysaccharides.
24815400	7	54	contain	had	991:993	arg2	activities					1014:1023	better antioxidant activities	995:1023	better antioxidant activities	995:1023	PL-A and PL-N had better antioxidant activities and could thus be developed as potential natural antioxidant agents for applications in food additives and biomedical industries.
24815400	7	54	contain	had	991:993	arg1	PL-N					986:989	PL-N	986:989	PL-N	986:989	PL-A and PL-N had better antioxidant activities and could thus be developed as potential natural antioxidant agents for applications in food additives and biomedical industries.
24815400	7	54	contain	had	991:993	arg1	PL-A					977:980	PL-A	977:980	PL-A	977:980	PL-A and PL-N had better antioxidant activities and could thus be developed as potential natural antioxidant agents for applications in food additives and biomedical industries.
24815400	7	54	contain	had	991:993	arg1	agents					1086:1091	potential natural antioxidant agents	1056:1091	potential natural antioxidant agents for applications in food additives and biomedical industries	1056:1152	PL-A and PL-N had better antioxidant activities and could thus be developed as potential natural antioxidant agents for applications in food additives and biomedical industries.
25841382	8	0	dep	coli	1120:1123	arg1	aureus					1177:1182	Staphylococcus aureus	1162:1182	Staphylococcus aureus	1162:1182	We observed that the composites possess excellent antimicrobial activity against Gram negative bacteria Escherichia coli (E. coli) and Gram positive bacteria Staphylococcus aureus (S. aureus) than chitosan.
25841382	8	0	dep	coli	1120:1123	arg1	aureus					1188:1193	S. aureus	1185:1193	S. aureus	1185:1193	We observed that the composites possess excellent antimicrobial activity against Gram negative bacteria Escherichia coli (E. coli) and Gram positive bacteria Staphylococcus aureus (S. aureus) than chitosan.
25841382	8	0	dep	coli	1120:1123	arg1	coli					1129:1132	E. coli	1126:1132	E. coli	1126:1132	We observed that the composites possess excellent antimicrobial activity against Gram negative bacteria Escherichia coli (E. coli) and Gram positive bacteria Staphylococcus aureus (S. aureus) than chitosan.
25841382	1	1	theme	chitosan	144:151	arg1	matrix					153:158	the chitosan matrix	140:158	the chitosan matrix	140:158	ZnO nanoparticles are immobilized on the chitosan matrix by an in situ sol-gel conversion of precursor molecules in a single step.
25841382	5	2	from	immobilization	661:674	arg1	matrix					705:710	the matrix	701:710	the matrix	701:710	The direct evidence of the immobilization of nano ZnO particles in the matrix was identified by SEM.
25841382	7	3	contain	have	926:929	arg2	energy					949:954	the same band gap energy	931:954	the same band gap energy (3.28eV)	931:963	Optical studies proved that all the three composites studied have the same band gap energy (3.28eV) in agreement with the reported values.
25841382	7	3	contain	have	926:929	arg1	composites					907:916	all the three composites	893:916	all the three composites studied	893:924	Optical studies proved that all the three composites studied have the same band gap energy (3.28eV) in agreement with the reported values.
25841382	7	3	contain	have	926:929	arg2	3.28eV					957:962	3.28eV	957:962	3.28eV	957:962	Optical studies proved that all the three composites studied have the same band gap energy (3.28eV) in agreement with the reported values.
25841382	8	4	dep	bacteria	1099:1106	arg1	bacteria					1153:1160	Gram positive bacteria	1139:1160	Gram positive bacteria	1139:1160	We observed that the composites possess excellent antimicrobial activity against Gram negative bacteria Escherichia coli (E. coli) and Gram positive bacteria Staphylococcus aureus (S. aureus) than chitosan.
25841382	8	4	dep	bacteria	1099:1106	arg1	coli					1120:1123	Escherichia coli	1108:1123	Escherichia coli (E. coli)	1108:1133	We observed that the composites possess excellent antimicrobial activity against Gram negative bacteria Escherichia coli (E. coli) and Gram positive bacteria Staphylococcus aureus (S. aureus) than chitosan.
25841382	0	5	theme	antimicrobial	82:94	arg1	agent					96:100	an antimicrobial agent	79:100	an antimicrobial agent	79:100	Applications of chitosan powder with in situ synthesized nano ZnO particles as an antimicrobial agent.
25841382	4	6	theme	band	483:486	arg1	width					488:492	the band width	479:492	the band width corresponding to OH and NH2 group in the composites	479:544	The observed decrease in the band width corresponding to OH and NH2 group in the composites is ascribed to the reduction of hydrogen bond due to the presence of ZnO nanoparticles.
25841382	6	7	theme	size	756:759	arg1	values					761:766	The average particle size values	735:766	The average particle size values obtained for the nanoparticles, using Debye-Scherrer equation from XRD,	735:838	The average particle size values obtained for the nanoparticles, using Debye-Scherrer equation from XRD, is in the range 10-18nm.
25841382	8	8	theme	negative	1090:1097	arg1	bacteria					1099:1106	Gram negative bacteria Escherichia coli (E. coli) and Gram positive bacteria Staphylococcus aureus (S. aureus)	1085:1194	Gram negative bacteria Escherichia coli (E. coli) and Gram positive bacteria Staphylococcus aureus (S. aureus)	1085:1194	We observed that the composites possess excellent antimicrobial activity against Gram negative bacteria Escherichia coli (E. coli) and Gram positive bacteria Staphylococcus aureus (S. aureus) than chitosan.
25841382	5	9	theme	ZnO	684:686	arg1	particles					688:696	nano ZnO particles	679:696	nano ZnO particles	679:696	The direct evidence of the immobilization of nano ZnO particles in the matrix was identified by SEM.
25841382	6	10	theme	particle	747:754	arg1	size					756:759	average particle size	739:759	The average particle size values obtained for the nanoparticles, using Debye-Scherrer equation from XRD,	735:838	The average particle size values obtained for the nanoparticles, using Debye-Scherrer equation from XRD, is in the range 10-18nm.
25841382	9	11	theme	low-cost	1275:1282	arg1	powder					1294:1299	the biocompatible, eco-friendly and low-cost composite powder	1239:1299	the biocompatible, eco-friendly and low-cost composite powder	1239:1299	Based on the above studies, the biocompatible, eco-friendly and low-cost composite powder could be applied in various fields as an antimicrobial agent.
25841382	5	12	theme	particles	688:696	arg1	immobilization					661:674	the immobilization	657:674	the immobilization of nano ZnO particles in the matrix	657:710	The direct evidence of the immobilization of nano ZnO particles in the matrix was identified by SEM.
25841382	2	13	theme	same	328:331	arg1	quantity					333:340	same quantity	328:340	same quantity of chitosan and zinc acetate dihydrate	328:379	Three different composites are prepared by varying the concentration of sodium hydroxide with same quantity of chitosan and zinc acetate dihydrate.
25841382	4	14	from	decrease	467:474	arg1	width					488:492	the band width	479:492	the band width corresponding to OH and NH2 group in the composites	479:544	The observed decrease in the band width corresponding to OH and NH2 group in the composites is ascribed to the reduction of hydrogen bond due to the presence of ZnO nanoparticles.
25841382	6	15	theme	average	739:745	arg1	size					756:759	average particle size	739:759	The average particle size values obtained for the nanoparticles, using Debye-Scherrer equation from XRD,	735:838	The average particle size values obtained for the nanoparticles, using Debye-Scherrer equation from XRD, is in the range 10-18nm.
25841382	1	16	theme	in	166:167	arg1	conversion					182:191	an in situ sol-gel conversion	163:191	an in situ sol-gel conversion of precursor molecules in a single step	163:231	ZnO nanoparticles are immobilized on the chitosan matrix by an in situ sol-gel conversion of precursor molecules in a single step.
25841382	9	17	theme	antimicrobial	1342:1354	arg1	agent					1356:1360	an antimicrobial agent	1339:1360	an antimicrobial agent	1339:1360	Based on the above studies, the biocompatible, eco-friendly and low-cost composite powder could be applied in various fields as an antimicrobial agent.
25841382	9	18	theme	various	1321:1327	arg1	fields					1329:1334	various fields	1321:1334	various fields as an antimicrobial agent	1321:1360	Based on the above studies, the biocompatible, eco-friendly and low-cost composite powder could be applied in various fields as an antimicrobial agent.
25841382	2	19	theme	dihydrate	371:379	arg1	quantity					333:340	same quantity	328:340	same quantity of chitosan and zinc acetate dihydrate	328:379	Three different composites are prepared by varying the concentration of sodium hydroxide with same quantity of chitosan and zinc acetate dihydrate.
25841382	4	20	theme	observed	458:465	arg1	decrease					467:474	The observed decrease	454:474	The observed decrease in the band width corresponding to OH and NH2 group in the composites	454:544	The observed decrease in the band width corresponding to OH and NH2 group in the composites is ascribed to the reduction of hydrogen bond due to the presence of ZnO nanoparticles.
25841382	8	21	theme	antimicrobial	1054:1066	arg1	activity					1068:1075	excellent antimicrobial activity	1044:1075	excellent antimicrobial activity against Gram negative bacteria Escherichia coli (E. coli) and Gram positive bacteria Staphylococcus aureus (S. aureus) than chitosan	1044:1208	We observed that the composites possess excellent antimicrobial activity against Gram negative bacteria Escherichia coli (E. coli) and Gram positive bacteria Staphylococcus aureus (S. aureus) than chitosan.
25841382	9	22	theme	above	1224:1228	arg1	studies					1230:1236	the above studies	1220:1236	the above studies	1220:1236	Based on the above studies, the biocompatible, eco-friendly and low-cost composite powder could be applied in various fields as an antimicrobial agent.
25841382	2	23	theme	acetate	363:369	arg1	dihydrate					371:379	zinc acetate dihydrate	358:379	zinc acetate dihydrate	358:379	Three different composites are prepared by varying the concentration of sodium hydroxide with same quantity of chitosan and zinc acetate dihydrate.
25841382	9	24	theme	biocompatible	1243:1255	arg1	powder					1294:1299	the biocompatible, eco-friendly and low-cost composite powder	1239:1299	the biocompatible, eco-friendly and low-cost composite powder	1239:1299	Based on the above studies, the biocompatible, eco-friendly and low-cost composite powder could be applied in various fields as an antimicrobial agent.
25841382	6	25	from	XRD	835:837	arg1	equation					821:828	Debye-Scherrer equation	806:828	Debye-Scherrer equation from XRD	806:837	The average particle size values obtained for the nanoparticles, using Debye-Scherrer equation from XRD, is in the range 10-18nm.
25841382	2	26	theme	zinc	358:361	arg1	dihydrate					371:379	zinc acetate dihydrate	358:379	zinc acetate dihydrate	358:379	Three different composites are prepared by varying the concentration of sodium hydroxide with same quantity of chitosan and zinc acetate dihydrate.
25841382	0	27	theme	powder	25:30	arg1	Applications					0:11	Applications	0:11	Applications of chitosan powder with in situ synthesized nano ZnO particles as an antimicrobial agent.	0:101	Applications of chitosan powder with in situ synthesized nano ZnO particles as an antimicrobial agent.
25841382	4	28	theme	NH2	518:520	arg1	group					522:526	OH and NH2 group	511:526	group	522:526	The observed decrease in the band width corresponding to OH and NH2 group in the composites is ascribed to the reduction of hydrogen bond due to the presence of ZnO nanoparticles.
25841382	7	29	theme	Optical	865:871	arg1	studies					873:879	Optical studies	865:879	Optical studies	865:879	Optical studies proved that all the three composites studied have the same band gap energy (3.28eV) in agreement with the reported values.
25841382	1	30	theme	sol-gel	174:180	arg1	conversion					182:191	an in situ sol-gel conversion	163:191	an in situ sol-gel conversion of precursor molecules in a single step	163:231	ZnO nanoparticles are immobilized on the chitosan matrix by an in situ sol-gel conversion of precursor molecules in a single step.
25841382	0	31	theme	chitosan	16:23	arg1	powder					25:30	chitosan powder	16:30	chitosan powder	16:30	Applications of chitosan powder with in situ synthesized nano ZnO particles as an antimicrobial agent.
25841382	6	32	dep	nanoparticles	785:797	arg1	using					800:804	using	800:804	using Debye-Scherrer equation from XRD	800:837	The average particle size values obtained for the nanoparticles, using Debye-Scherrer equation from XRD, is in the range 10-18nm.
25841382	7	33	theme	reported	987:994	arg1	values					996:1001	the reported values	983:1001	the reported values	983:1001	Optical studies proved that all the three composites studied have the same band gap energy (3.28eV) in agreement with the reported values.
25841382	2	34	with	hydroxide	313:321	arg1	quantity					333:340	same quantity	328:340	same quantity of chitosan and zinc acetate dihydrate	328:379	Three different composites are prepared by varying the concentration of sodium hydroxide with same quantity of chitosan and zinc acetate dihydrate.
25841382	8	35	contain	possess	1036:1042	arg1	composites					1025:1034	the composites	1021:1034	the composites	1021:1034	We observed that the composites possess excellent antimicrobial activity against Gram negative bacteria Escherichia coli (E. coli) and Gram positive bacteria Staphylococcus aureus (S. aureus) than chitosan.
25841382	8	35	contain	possess	1036:1042	arg2	activity					1068:1075	excellent antimicrobial activity	1044:1075	excellent antimicrobial activity against Gram negative bacteria Escherichia coli (E. coli) and Gram positive bacteria Staphylococcus aureus (S. aureus) than chitosan	1044:1208	We observed that the composites possess excellent antimicrobial activity against Gram negative bacteria Escherichia coli (E. coli) and Gram positive bacteria Staphylococcus aureus (S. aureus) than chitosan.
25841382	4	36	theme	bond	587:590	arg1	reduction					565:573	the reduction	561:573	the reduction of hydrogen bond due to the presence of ZnO nanoparticles	561:631	The observed decrease in the band width corresponding to OH and NH2 group in the composites is ascribed to the reduction of hydrogen bond due to the presence of ZnO nanoparticles.
25841382	4	37	theme	nanoparticles	619:631	arg1	presence					603:610	the presence	599:610	the presence of ZnO nanoparticles	599:631	The observed decrease in the band width corresponding to OH and NH2 group in the composites is ascribed to the reduction of hydrogen bond due to the presence of ZnO nanoparticles.
25841382	5	38	theme	immobilization	661:674	arg1	evidence					645:652	The direct evidence	634:652	The direct evidence of the immobilization of nano ZnO particles in the matrix	634:710	The direct evidence of the immobilization of nano ZnO particles in the matrix was identified by SEM.
25841382	6	39	theme	Debye-Scherrer	806:819	arg1	equation					821:828	Debye-Scherrer equation	806:828	Debye-Scherrer equation from XRD	806:837	The average particle size values obtained for the nanoparticles, using Debye-Scherrer equation from XRD, is in the range 10-18nm.
25841382	1	40	dep	in	166:167	arg1	situ					169:172	situ	169:172	situ	169:172	ZnO nanoparticles are immobilized on the chitosan matrix by an in situ sol-gel conversion of precursor molecules in a single step.
25841382	4	41	theme	ZnO	615:617	arg1	nanoparticles					619:631	ZnO nanoparticles	615:631	ZnO nanoparticles	615:631	The observed decrease in the band width corresponding to OH and NH2 group in the composites is ascribed to the reduction of hydrogen bond due to the presence of ZnO nanoparticles.
25841382	4	42	from	group	522:526	arg1	composites					535:544	the composites	531:544	the composites	531:544	The observed decrease in the band width corresponding to OH and NH2 group in the composites is ascribed to the reduction of hydrogen bond due to the presence of ZnO nanoparticles.
25841382	7	43	theme	gap	945:947	arg1	3.28eV					957:962	3.28eV	957:962	3.28eV	957:962	Optical studies proved that all the three composites studied have the same band gap energy (3.28eV) in agreement with the reported values.
25841382	7	43	theme	gap	945:947	arg1	energy					949:954	the same band gap energy	931:954	the same band gap energy (3.28eV)	931:963	Optical studies proved that all the three composites studied have the same band gap energy (3.28eV) in agreement with the reported values.
25841382	1	44	theme	precursor	196:204	arg1	molecules					206:214	precursor molecules	196:214	precursor molecules	196:214	ZnO nanoparticles are immobilized on the chitosan matrix by an in situ sol-gel conversion of precursor molecules in a single step.
25841382	0	45	theme	synthesized	45:55	arg1	particles					66:74	in situ synthesized nano ZnO particles	37:74	in situ synthesized nano ZnO particles as an antimicrobial agent	37:100	Applications of chitosan powder with in situ synthesized nano ZnO particles as an antimicrobial agent.
25841382	5	46	theme	nano	679:682	arg1	particles					688:696	nano ZnO particles	679:696	nano ZnO particles	679:696	The direct evidence of the immobilization of nano ZnO particles in the matrix was identified by SEM.
25841382	8	47	theme	excellent	1044:1052	arg1	activity					1068:1075	excellent antimicrobial activity	1044:1075	excellent antimicrobial activity against Gram negative bacteria Escherichia coli (E. coli) and Gram positive bacteria Staphylococcus aureus (S. aureus) than chitosan	1044:1208	We observed that the composites possess excellent antimicrobial activity against Gram negative bacteria Escherichia coli (E. coli) and Gram positive bacteria Staphylococcus aureus (S. aureus) than chitosan.
25841382	1	48	theme	ZnO	103:105	arg1	nanoparticles					107:119	ZnO nanoparticles	103:119	ZnO nanoparticles	103:119	ZnO nanoparticles are immobilized on the chitosan matrix by an in situ sol-gel conversion of precursor molecules in a single step.
25841382	8	49	theme	Gram	1139:1142	arg1	bacteria					1153:1160	Gram positive bacteria	1139:1160	Gram positive bacteria	1139:1160	We observed that the composites possess excellent antimicrobial activity against Gram negative bacteria Escherichia coli (E. coli) and Gram positive bacteria Staphylococcus aureus (S. aureus) than chitosan.
25841382	9	50	theme	composite	1284:1292	arg1	powder					1294:1299	the biocompatible, eco-friendly and low-cost composite powder	1239:1299	the biocompatible, eco-friendly and low-cost composite powder	1239:1299	Based on the above studies, the biocompatible, eco-friendly and low-cost composite powder could be applied in various fields as an antimicrobial agent.
25841382	7	51	theme	band	940:943	arg1	3.28eV					957:962	3.28eV	957:962	3.28eV	957:962	Optical studies proved that all the three composites studied have the same band gap energy (3.28eV) in agreement with the reported values.
25841382	7	51	theme	band	940:943	arg1	energy					949:954	the same band gap energy	931:954	the same band gap energy (3.28eV)	931:963	Optical studies proved that all the three composites studied have the same band gap energy (3.28eV) in agreement with the reported values.
25841382	7	52	theme	same	935:938	arg1	3.28eV					957:962	3.28eV	957:962	3.28eV	957:962	Optical studies proved that all the three composites studied have the same band gap energy (3.28eV) in agreement with the reported values.
25841382	7	52	theme	same	935:938	arg1	energy					949:954	the same band gap energy	931:954	the same band gap energy (3.28eV)	931:963	Optical studies proved that all the three composites studied have the same band gap energy (3.28eV) in agreement with the reported values.
25841382	7	53	with	agreement	968:976	arg1	values					996:1001	the reported values	983:1001	the reported values	983:1001	Optical studies proved that all the three composites studied have the same band gap energy (3.28eV) in agreement with the reported values.
25841382	4	54	theme	OH	511:512	arg1	group					522:526	OH and NH2 group	511:526	group	522:526	The observed decrease in the band width corresponding to OH and NH2 group in the composites is ascribed to the reduction of hydrogen bond due to the presence of ZnO nanoparticles.
25841382	5	55	theme	direct	638:643	arg1	evidence					645:652	The direct evidence	634:652	The direct evidence of the immobilization of nano ZnO particles in the matrix	634:710	The direct evidence of the immobilization of nano ZnO particles in the matrix was identified by SEM.
25841382	8	56	theme	Gram	1085:1088	arg1	bacteria					1099:1106	Gram negative bacteria Escherichia coli (E. coli) and Gram positive bacteria Staphylococcus aureus (S. aureus)	1085:1194	Gram negative bacteria Escherichia coli (E. coli) and Gram positive bacteria Staphylococcus aureus (S. aureus)	1085:1194	We observed that the composites possess excellent antimicrobial activity against Gram negative bacteria Escherichia coli (E. coli) and Gram positive bacteria Staphylococcus aureus (S. aureus) than chitosan.
25841382	2	57	theme	different	240:248	arg1	composites					250:259	Three different composites	234:259	Three different composites	234:259	Three different composites are prepared by varying the concentration of sodium hydroxide with same quantity of chitosan and zinc acetate dihydrate.
25841382	0	58	with	Applications	0:11	arg1	particles					66:74	in situ synthesized nano ZnO particles	37:74	in situ synthesized nano ZnO particles as an antimicrobial agent	37:100	Applications of chitosan powder with in situ synthesized nano ZnO particles as an antimicrobial agent.
25841382	2	59	theme	hydroxide	313:321	arg1	concentration					289:301	the concentration	285:301	the concentration of sodium hydroxide with same quantity of chitosan and zinc acetate dihydrate	285:379	Three different composites are prepared by varying the concentration of sodium hydroxide with same quantity of chitosan and zinc acetate dihydrate.
25841382	1	60	theme	molecules	206:214	arg1	conversion					182:191	an in situ sol-gel conversion	163:191	an in situ sol-gel conversion of precursor molecules in a single step	163:231	ZnO nanoparticles are immobilized on the chitosan matrix by an in situ sol-gel conversion of precursor molecules in a single step.
25841382	1	61	from	conversion	182:191	arg1	step					228:231	a single step	219:231	a single step	219:231	ZnO nanoparticles are immobilized on the chitosan matrix by an in situ sol-gel conversion of precursor molecules in a single step.
25841382	2	62	theme	sodium	306:311	arg1	hydroxide					313:321	sodium hydroxide	306:321	sodium hydroxide with same quantity of chitosan and zinc acetate dihydrate	306:379	Three different composites are prepared by varying the concentration of sodium hydroxide with same quantity of chitosan and zinc acetate dihydrate.
25841382	8	63	theme	positive	1144:1151	arg1	bacteria					1153:1160	Gram positive bacteria	1139:1160	Gram positive bacteria	1139:1160	We observed that the composites possess excellent antimicrobial activity against Gram negative bacteria Escherichia coli (E. coli) and Gram positive bacteria Staphylococcus aureus (S. aureus) than chitosan.
25841382	5	64	from	evidence	645:652	arg1	matrix					705:710	the matrix	701:710	the matrix	701:710	The direct evidence of the immobilization of nano ZnO particles in the matrix was identified by SEM.
25841382	0	65	theme	ZnO	62:64	arg1	particles					66:74	in situ synthesized nano ZnO particles	37:74	in situ synthesized nano ZnO particles as an antimicrobial agent	37:100	Applications of chitosan powder with in situ synthesized nano ZnO particles as an antimicrobial agent.
25841382	5	66	from	matrix	705:710	arg1	evidence					645:652	The direct evidence	634:652	The direct evidence of the immobilization of nano ZnO particles in the matrix	634:710	The direct evidence of the immobilization of nano ZnO particles in the matrix was identified by SEM.
25841382	4	67	theme	due	592:594	arg1	bond					587:590	hydrogen bond	578:590	hydrogen bond due to the presence of ZnO nanoparticles	578:631	The observed decrease in the band width corresponding to OH and NH2 group in the composites is ascribed to the reduction of hydrogen bond due to the presence of ZnO nanoparticles.
25841382	3	68	theme	UV-visible	425:434	arg1	spectra					436:442	UV-visible spectra	425:442	UV-visible spectra	425:442	The composites were characterized by FTIR, UV-visible spectra, and XRD.
25841382	4	69	theme	hydrogen	578:585	arg1	bond					587:590	hydrogen bond	578:590	hydrogen bond due to the presence of ZnO nanoparticles	578:631	The observed decrease in the band width corresponding to OH and NH2 group in the composites is ascribed to the reduction of hydrogen bond due to the presence of ZnO nanoparticles.
25841382	0	70	theme	nano	57:60	arg1	particles					66:74	in situ synthesized nano ZnO particles	37:74	in situ synthesized nano ZnO particles as an antimicrobial agent	37:100	Applications of chitosan powder with in situ synthesized nano ZnO particles as an antimicrobial agent.
25841382	9	71	theme	eco-friendly	1258:1269	arg1	powder					1294:1299	the biocompatible, eco-friendly and low-cost composite powder	1239:1299	the biocompatible, eco-friendly and low-cost composite powder	1239:1299	Based on the above studies, the biocompatible, eco-friendly and low-cost composite powder could be applied in various fields as an antimicrobial agent.
25841382	2	72	theme	chitosan	345:352	arg1	quantity					333:340	same quantity	328:340	same quantity of chitosan and zinc acetate dihydrate	328:379	Three different composites are prepared by varying the concentration of sodium hydroxide with same quantity of chitosan and zinc acetate dihydrate.
25841382	1	73	theme	single	221:226	arg1	step					228:231	a single step	219:231	a single step	219:231	ZnO nanoparticles are immobilized on the chitosan matrix by an in situ sol-gel conversion of precursor molecules in a single step.
29023716	7	0	theme	microbiological	1237:1251	arg1	contamination					1253:1265	microbiological contamination	1237:1265	microbiological contamination	1237:1265	The values of hygroscopicity (g adsorbed water/100 g of dry matter) indicated the lyophilized liposomes were able to decrease the capacity of the agglomerated cornstarch to absorb water, decreasing the possibility of microbiological contamination.
29023716	11	1	theme	PRACTICAL	1905:1913	arg1	APPLICATION					1915:1925	PRACTICAL APPLICATION	1905:1925	PRACTICAL APPLICATION This study is an unprecedent association of 2 technologies, nanoencapsulation and wet agglomeration,	1905:2026	PRACTICAL APPLICATION This study is an unprecedent association of 2 technologies, nanoencapsulation and wet agglomeration, here used together to enrich cornstarch with quercetin.
29023716	9	2	theme	cornstarch	1459:1468	arg1	properties					1423:1432	The pasting properties	1411:1432	The pasting properties of enriched agglomerated cornstarch	1411:1468	The pasting properties of enriched agglomerated cornstarch decreased the pasting temperature about 10 °C, and the cornstarch agglomerated with 8% (w/w) of quercetin-loaded lyophilized liposomes showed no significance difference in the peak viscosity.
29023716	4	3	theme	native	634:639	arg1	cornstarch					641:650	native cornstarch	634:650	native cornstarch	634:650	In this study, the binary mixtures containing microparticles of native cornstarch and nanoparticles of quercetin liposomes were aggregated in high-shear batches, using maltodextrin solution as binder agent.
29023716	2	4	theme	high	295:298	arg1	activity					312:319	high antioxidant activity	295:319	high antioxidant activity	295:319	Quercetin is a flavonoid with high antioxidant activity that provides benefits to health.
29023716	4	5	theme	high-shear	712:721	arg1	batches					723:729	high-shear batches	712:729	high-shear batches	712:729	In this study, the binary mixtures containing microparticles of native cornstarch and nanoparticles of quercetin liposomes were aggregated in high-shear batches, using maltodextrin solution as binder agent.
29023716	5	6	theme	liposomes	882:890	arg1	%					833:833	22%	831:833	22%	831:833	The cornstarch was enriched by agglomeration with 8%, 22%, and 30% (w/w) of quercetin-loaded lyophilized liposomes and the physical properties were evaluated.
29023716	5	6	theme	liposomes	882:890	arg1	liposomes					882:890	quercetin-loaded lyophilized liposomes	853:890	quercetin-loaded lyophilized liposomes	853:890	The cornstarch was enriched by agglomeration with 8%, 22%, and 30% (w/w) of quercetin-loaded lyophilized liposomes and the physical properties were evaluated.
29023716	5	6	theme	liposomes	882:890	arg1	w/w					845:847	w/w	845:847	w/w	845:847	The cornstarch was enriched by agglomeration with 8%, 22%, and 30% (w/w) of quercetin-loaded lyophilized liposomes and the physical properties were evaluated.
29023716	5	6	theme	liposomes	882:890	arg1	%					842:842	30%	840:842	30%	840:842	The cornstarch was enriched by agglomeration with 8%, 22%, and 30% (w/w) of quercetin-loaded lyophilized liposomes and the physical properties were evaluated.
29023716	5	6	theme	liposomes	882:890	arg1	%					828:828	8%	827:828	8%	827:828	The cornstarch was enriched by agglomeration with 8%, 22%, and 30% (w/w) of quercetin-loaded lyophilized liposomes and the physical properties were evaluated.
29023716	4	7	theme	liposomes	683:691	arg1	nanoparticles					656:668	nanoparticles	656:668	nanoparticles	656:668	In this study, the binary mixtures containing microparticles of native cornstarch and nanoparticles of quercetin liposomes were aggregated in high-shear batches, using maltodextrin solution as binder agent.
29023716	4	7	theme	liposomes	683:691	arg1	mixtures					596:603	the binary mixtures	585:603	the binary mixtures containing microparticles of native cornstarch	585:650	In this study, the binary mixtures containing microparticles of native cornstarch and nanoparticles of quercetin liposomes were aggregated in high-shear batches, using maltodextrin solution as binder agent.
29023716	7	8	theme	dry	1076:1078	arg1	matter					1080:1085	dry matter	1076:1085	dry matter	1076:1085	The values of hygroscopicity (g adsorbed water/100 g of dry matter) indicated the lyophilized liposomes were able to decrease the capacity of the agglomerated cornstarch to absorb water, decreasing the possibility of microbiological contamination.
29023716	13	9	theme	value	2351:2355	arg1	goals					2262:2266	The goals	2258:2266	The goals	2258:2266	The goals were both the improvement of the nutritional quality and the increase of the added value of the cornstarch.
29023716	13	9	theme	value	2351:2355	arg1	increase					2329:2336	the increase	2325:2336	the increase of the added value of the cornstarch	2325:2373	The goals were both the improvement of the nutritional quality and the increase of the added value of the cornstarch.
29023716	13	9	theme	value	2351:2355	arg1	improvement					2282:2292	the improvement	2278:2292	the improvement of the nutritional quality	2278:2319	The goals were both the improvement of the nutritional quality and the increase of the added value of the cornstarch.
29023716	8	10	theme	liposomes	1313:1321	arg1	addition					1272:1279	The addition	1268:1279	The addition of quercetin-loaded lyophilized liposomes	1268:1321	The addition of quercetin-loaded lyophilized liposomes improved the flowability and turned the powder (agglomerated cornstarch) less cohesive.
29023716	8	11	theme	lyophilized	1301:1311	arg1	liposomes					1313:1321	quercetin-loaded lyophilized liposomes	1284:1321	quercetin-loaded lyophilized liposomes	1284:1321	The addition of quercetin-loaded lyophilized liposomes improved the flowability and turned the powder (agglomerated cornstarch) less cohesive.
29023716	11	12	theme	wet	2009:2011	arg1	agglomeration					2013:2025	wet agglomeration	2009:2025	wet agglomeration	2009:2025	PRACTICAL APPLICATION This study is an unprecedent association of 2 technologies, nanoencapsulation and wet agglomeration, here used together to enrich cornstarch with quercetin.
29023716	11	12	theme	wet	2009:2011	arg1	technologies					1973:1984	2 technologies	1971:1984	2 technologies	1971:1984	PRACTICAL APPLICATION This study is an unprecedent association of 2 technologies, nanoencapsulation and wet agglomeration, here used together to enrich cornstarch with quercetin.
29023716	1	13	theme	hydrophobic	227:237	arg1	bioctives					239:247	hydrophobic bioctives	227:247	hydrophobic bioctives	227:247	Liposomes are colloidal structures capable of encapsulating, protecting, and releasing hydrophobic bioctives, as flavonoids.
29023716	7	14	theme	adsorbed	1052:1059	arg1	g					1071:1071	g adsorbed water/100 g	1050:1071	g adsorbed water/100 g of dry matter	1050:1085	The values of hygroscopicity (g adsorbed water/100 g of dry matter) indicated the lyophilized liposomes were able to decrease the capacity of the agglomerated cornstarch to absorb water, decreasing the possibility of microbiological contamination.
29023716	11	15	dep	APPLICATION	1915:1925	arg1	study					1932:1936	This study	1927:1936	This study	1927:1936	PRACTICAL APPLICATION This study is an unprecedent association of 2 technologies, nanoencapsulation and wet agglomeration, here used together to enrich cornstarch with quercetin.
29023716	11	15	dep	APPLICATION	1915:1925	arg1	association					1956:1966	an unprecedent association	1941:1966	an unprecedent association of 2 technologies, nanoencapsulation and wet agglomeration,	1941:2026	PRACTICAL APPLICATION This study is an unprecedent association of 2 technologies, nanoencapsulation and wet agglomeration, here used together to enrich cornstarch with quercetin.
29023716	10	16	theme	retrogradation	1857:1870	arg1	levels					1847:1852	low levels	1843:1852	low levels of retrogradation of granules for their stability	1843:1902	Agglomeration of cornstarch with more than 8% (w/w) lyophilized liposomes decreased the tendency of starch to retrograde, which is very interesting for food products which requires low levels of retrogradation of granules for their stability.
29023716	2	17	with	flavonoid	280:288	arg1	activity					312:319	high antioxidant activity	295:319	high antioxidant activity	295:319	Quercetin is a flavonoid with high antioxidant activity that provides benefits to health.
29023716	4	18	theme	maltodextrin	738:749	arg1	solution					751:758	maltodextrin solution	738:758	maltodextrin solution	738:758	In this study, the binary mixtures containing microparticles of native cornstarch and nanoparticles of quercetin liposomes were aggregated in high-shear batches, using maltodextrin solution as binder agent.
29023716	1	19	theme	colloidal	154:162	arg1	Liposomes					140:148	Liposomes	140:148	Liposomes	140:148	Liposomes are colloidal structures capable of encapsulating, protecting, and releasing hydrophobic bioctives, as flavonoids.
29023716	1	19	theme	colloidal	154:162	arg1	structures					164:173	colloidal structures	154:173	colloidal structures capable of encapsulating, protecting, and releasing hydrophobic bioctives	154:247	Liposomes are colloidal structures capable of encapsulating, protecting, and releasing hydrophobic bioctives, as flavonoids.
29023716	1	20	theme	capable	175:181	arg1	Liposomes					140:148	Liposomes	140:148	Liposomes	140:148	Liposomes are colloidal structures capable of encapsulating, protecting, and releasing hydrophobic bioctives, as flavonoids.
29023716	1	20	theme	capable	175:181	arg1	structures					164:173	colloidal structures	154:173	colloidal structures capable of encapsulating, protecting, and releasing hydrophobic bioctives	154:247	Liposomes are colloidal structures capable of encapsulating, protecting, and releasing hydrophobic bioctives, as flavonoids.
29023716	8	21	theme	agglomerated	1371:1382	arg1	cornstarch					1384:1393	agglomerated cornstarch	1371:1393	agglomerated cornstarch	1371:1393	The addition of quercetin-loaded lyophilized liposomes improved the flowability and turned the powder (agglomerated cornstarch) less cohesive.
29023716	8	21	theme	agglomerated	1371:1382	arg1	powder					1363:1368	the powder	1359:1368	the powder (agglomerated cornstarch) less cohesive	1359:1408	The addition of quercetin-loaded lyophilized liposomes improved the flowability and turned the powder (agglomerated cornstarch) less cohesive.
29023716	0	22	theme	Binary	98:103	arg1	Mixtures					105:112	Particulate Binary Mixtures	86:112	Particulate Binary Mixtures Using High Shear Process	86:137	Production of Cornstarch Granules Enriched with Quercetin Liposomes by Aggregation of Particulate Binary Mixtures Using High Shear Process.
29023716	9	23	theme	quercetin-loaded	1566:1581	arg1	liposomes					1595:1603	quercetin-loaded lyophilized liposomes	1566:1603	quercetin-loaded lyophilized liposomes	1566:1603	The pasting properties of enriched agglomerated cornstarch decreased the pasting temperature about 10 °C, and the cornstarch agglomerated with 8% (w/w) of quercetin-loaded lyophilized liposomes showed no significance difference in the peak viscosity.
29023716	3	24	theme	wet-agglomeration	359:375	arg1	useful					415:420	useful	415:420	useful	415:420	The wet-agglomeration processes in high-shear equipment are useful to produce granules from binary mixtures, obtaining a powder with homogeneous composition and without segregation or elutriation of fine particles.
29023716	3	24	theme	wet-agglomeration	359:375	arg1	processes					377:385	The wet-agglomeration processes	355:385	The wet-agglomeration processes in high-shear equipment	355:409	The wet-agglomeration processes in high-shear equipment are useful to produce granules from binary mixtures, obtaining a powder with homogeneous composition and without segregation or elutriation of fine particles.
29023716	9	25	theme	liposomes	1595:1603	arg1	liposomes					1595:1603	quercetin-loaded lyophilized liposomes	1566:1603	quercetin-loaded lyophilized liposomes	1566:1603	The pasting properties of enriched agglomerated cornstarch decreased the pasting temperature about 10 °C, and the cornstarch agglomerated with 8% (w/w) of quercetin-loaded lyophilized liposomes showed no significance difference in the peak viscosity.
29023716	9	25	theme	liposomes	1595:1603	arg1	w/w					1558:1560	w/w	1558:1560	w/w	1558:1560	The pasting properties of enriched agglomerated cornstarch decreased the pasting temperature about 10 °C, and the cornstarch agglomerated with 8% (w/w) of quercetin-loaded lyophilized liposomes showed no significance difference in the peak viscosity.
29023716	9	25	theme	liposomes	1595:1603	arg1	%					1555:1555	8%	1554:1555	8% (w/w) of quercetin-loaded lyophilized liposomes	1554:1603	The pasting properties of enriched agglomerated cornstarch decreased the pasting temperature about 10 °C, and the cornstarch agglomerated with 8% (w/w) of quercetin-loaded lyophilized liposomes showed no significance difference in the peak viscosity.
29023716	3	26	theme	particles	559:567	arg1	segregation					524:534	segregation	524:534	segregation	524:534	The wet-agglomeration processes in high-shear equipment are useful to produce granules from binary mixtures, obtaining a powder with homogeneous composition and without segregation or elutriation of fine particles.
29023716	3	26	theme	particles	559:567	arg1	elutriation					539:549	elutriation	539:549	elutriation	539:549	The wet-agglomeration processes in high-shear equipment are useful to produce granules from binary mixtures, obtaining a powder with homogeneous composition and without segregation or elutriation of fine particles.
29023716	8	27	theme	cohesive	1401:1408	arg1	cornstarch					1384:1393	agglomerated cornstarch	1371:1393	agglomerated cornstarch	1371:1393	The addition of quercetin-loaded lyophilized liposomes improved the flowability and turned the powder (agglomerated cornstarch) less cohesive.
29023716	8	27	theme	cohesive	1401:1408	arg1	powder					1363:1368	the powder	1359:1368	the powder (agglomerated cornstarch) less cohesive	1359:1408	The addition of quercetin-loaded lyophilized liposomes improved the flowability and turned the powder (agglomerated cornstarch) less cohesive.
29023716	7	28	theme	cornstarch	1179:1188	arg1	capacity					1150:1157	the capacity	1146:1157	the capacity of the agglomerated cornstarch to absorb water	1146:1204	The values of hygroscopicity (g adsorbed water/100 g of dry matter) indicated the lyophilized liposomes were able to decrease the capacity of the agglomerated cornstarch to absorb water, decreasing the possibility of microbiological contamination.
29023716	3	29	theme	homogeneous	488:498	arg1	composition					500:510	homogeneous composition	488:510	homogeneous composition	488:510	The wet-agglomeration processes in high-shear equipment are useful to produce granules from binary mixtures, obtaining a powder with homogeneous composition and without segregation or elutriation of fine particles.
29023716	0	30	theme	Cornstarch	14:23	arg1	Granules					25:32	Cornstarch Granules	14:32	Cornstarch Granules	14:32	Production of Cornstarch Granules Enriched with Quercetin Liposomes by Aggregation of Particulate Binary Mixtures Using High Shear Process.
29023716	11	31	used	used	2033:2036	arg2	APPLICATION					1915:1925	PRACTICAL APPLICATION	1905:1925	PRACTICAL APPLICATION This study is an unprecedent association of 2 technologies, nanoencapsulation and wet agglomeration,	1905:2026	PRACTICAL APPLICATION This study is an unprecedent association of 2 technologies, nanoencapsulation and wet agglomeration, here used together to enrich cornstarch with quercetin.
29023716	4	32	theme	binary	589:594	arg1	mixtures					596:603	the binary mixtures	585:603	the binary mixtures containing microparticles of native cornstarch	585:650	In this study, the binary mixtures containing microparticles of native cornstarch and nanoparticles of quercetin liposomes were aggregated in high-shear batches, using maltodextrin solution as binder agent.
29023716	12	33	theme	quercetin-loaded	2218:2233	arg1	liposomes					2247:2255	quercetin-loaded lyophilized liposomes	2218:2255	quercetin-loaded lyophilized liposomes	2218:2255	The agglomeration process was used to obtain granules of cornstarch, an ingredient extremely used in the food industry, enriched with quercetin-loaded lyophilized liposomes.
29023716	9	34	theme	pasting	1484:1490	arg1	temperature					1492:1502	the pasting temperature	1480:1502	the pasting temperature about 10 °C	1480:1514	The pasting properties of enriched agglomerated cornstarch decreased the pasting temperature about 10 °C, and the cornstarch agglomerated with 8% (w/w) of quercetin-loaded lyophilized liposomes showed no significance difference in the peak viscosity.
29023716	10	35	theme	granules	1875:1882	arg1	retrogradation					1857:1870	retrogradation	1857:1870	retrogradation of granules for their stability	1857:1902	Agglomeration of cornstarch with more than 8% (w/w) lyophilized liposomes decreased the tendency of starch to retrograde, which is very interesting for food products which requires low levels of retrogradation of granules for their stability.
29023716	0	36	theme	Quercetin	48:56	arg1	Liposomes					58:66	Quercetin Liposomes	48:66	Quercetin Liposomes	48:66	Production of Cornstarch Granules Enriched with Quercetin Liposomes by Aggregation of Particulate Binary Mixtures Using High Shear Process.
29023716	9	37	theme	pasting	1415:1421	arg1	properties					1423:1432	The pasting properties	1411:1432	The pasting properties of enriched agglomerated cornstarch	1411:1468	The pasting properties of enriched agglomerated cornstarch decreased the pasting temperature about 10 °C, and the cornstarch agglomerated with 8% (w/w) of quercetin-loaded lyophilized liposomes showed no significance difference in the peak viscosity.
29023716	10	38	with	Agglomeration	1662:1674	arg1	liposomes					1726:1734	more than 8% (w/w) lyophilized liposomes	1695:1734	more than 8% (w/w) lyophilized liposomes	1695:1734	Agglomeration of cornstarch with more than 8% (w/w) lyophilized liposomes decreased the tendency of starch to retrograde, which is very interesting for food products which requires low levels of retrogradation of granules for their stability.
29023716	13	39	theme	nutritional	2301:2311	arg1	quality					2313:2319	the nutritional quality	2297:2319	the nutritional quality	2297:2319	The goals were both the improvement of the nutritional quality and the increase of the added value of the cornstarch.
29023716	4	40	theme	cornstarch	641:650	arg1	microparticles					616:629	microparticles	616:629	microparticles of native cornstarch	616:650	In this study, the binary mixtures containing microparticles of native cornstarch and nanoparticles of quercetin liposomes were aggregated in high-shear batches, using maltodextrin solution as binder agent.
29023716	3	41	theme	fine	554:557	arg1	particles					559:567	fine particles	554:567	fine particles	554:567	The wet-agglomeration processes in high-shear equipment are useful to produce granules from binary mixtures, obtaining a powder with homogeneous composition and without segregation or elutriation of fine particles.
29023716	9	42	theme	agglomerated	1446:1457	arg1	cornstarch					1459:1468	enriched agglomerated cornstarch	1437:1468	enriched agglomerated cornstarch	1437:1468	The pasting properties of enriched agglomerated cornstarch decreased the pasting temperature about 10 °C, and the cornstarch agglomerated with 8% (w/w) of quercetin-loaded lyophilized liposomes showed no significance difference in the peak viscosity.
29023716	7	43	theme	contamination	1253:1265	arg1	possibility					1222:1232	the possibility	1218:1232	the possibility of microbiological contamination	1218:1265	The values of hygroscopicity (g adsorbed water/100 g of dry matter) indicated the lyophilized liposomes were able to decrease the capacity of the agglomerated cornstarch to absorb water, decreasing the possibility of microbiological contamination.
29023716	7	44	dep	hygroscopicity	1034:1047	arg1	g					1071:1071	g adsorbed water/100 g	1050:1071	g adsorbed water/100 g of dry matter	1050:1085	The values of hygroscopicity (g adsorbed water/100 g of dry matter) indicated the lyophilized liposomes were able to decrease the capacity of the agglomerated cornstarch to absorb water, decreasing the possibility of microbiological contamination.
29023716	7	45	theme	lyophilized	1102:1112	arg1	liposomes					1114:1122	the lyophilized liposomes	1098:1122	the lyophilized liposomes	1098:1122	The values of hygroscopicity (g adsorbed water/100 g of dry matter) indicated the lyophilized liposomes were able to decrease the capacity of the agglomerated cornstarch to absorb water, decreasing the possibility of microbiological contamination.
29023716	7	45	theme	lyophilized	1102:1112	arg1	able					1129:1132	able	1129:1132	able	1129:1132	The values of hygroscopicity (g adsorbed water/100 g of dry matter) indicated the lyophilized liposomes were able to decrease the capacity of the agglomerated cornstarch to absorb water, decreasing the possibility of microbiological contamination.
29023716	2	46	theme	antioxidant	300:310	arg1	activity					312:319	high antioxidant activity	295:319	high antioxidant activity	295:319	Quercetin is a flavonoid with high antioxidant activity that provides benefits to health.
29023716	5	47	theme	lyophilized	870:880	arg1	liposomes					882:890	quercetin-loaded lyophilized liposomes	853:890	quercetin-loaded lyophilized liposomes	853:890	The cornstarch was enriched by agglomeration with 8%, 22%, and 30% (w/w) of quercetin-loaded lyophilized liposomes and the physical properties were evaluated.
29023716	13	48	theme	cornstarch	2364:2373	arg1	value					2351:2355	the added value	2341:2355	the added value of the cornstarch	2341:2373	The goals were both the improvement of the nutritional quality and the increase of the added value of the cornstarch.
29023716	7	49	theme	matter	1080:1085	arg1	g					1071:1071	g adsorbed water/100 g	1050:1071	g adsorbed water/100 g of dry matter	1050:1085	The values of hygroscopicity (g adsorbed water/100 g of dry matter) indicated the lyophilized liposomes were able to decrease the capacity of the agglomerated cornstarch to absorb water, decreasing the possibility of microbiological contamination.
29023716	10	50	theme	food	1814:1817	arg1	products					1819:1826	food products	1814:1826	food products which requires low levels of retrogradation of granules for their stability	1814:1902	Agglomeration of cornstarch with more than 8% (w/w) lyophilized liposomes decreased the tendency of starch to retrograde, which is very interesting for food products which requires low levels of retrogradation of granules for their stability.
29023716	3	51	with	powder	476:481	arg1	composition					500:510	homogeneous composition	488:510	homogeneous composition	488:510	The wet-agglomeration processes in high-shear equipment are useful to produce granules from binary mixtures, obtaining a powder with homogeneous composition and without segregation or elutriation of fine particles.
29023716	13	52	theme	added	2345:2349	arg1	value					2351:2355	the added value	2341:2355	the added value of the cornstarch	2341:2373	The goals were both the improvement of the nutritional quality and the increase of the added value of the cornstarch.
29023716	4	53	theme	quercetin	673:681	arg1	liposomes					683:691	quercetin liposomes	673:691	quercetin liposomes	673:691	In this study, the binary mixtures containing microparticles of native cornstarch and nanoparticles of quercetin liposomes were aggregated in high-shear batches, using maltodextrin solution as binder agent.
29023716	10	54	theme	cornstarch	1679:1688	arg1	Agglomeration					1662:1674	Agglomeration	1662:1674	Agglomeration of cornstarch with more than 8% (w/w) lyophilized liposomes	1662:1734	Agglomeration of cornstarch with more than 8% (w/w) lyophilized liposomes decreased the tendency of starch to retrograde, which is very interesting for food products which requires low levels of retrogradation of granules for their stability.
29023716	7	55	theme	water/100	1061:1069	arg1	g					1071:1071	g adsorbed water/100 g	1050:1071	g adsorbed water/100 g of dry matter	1050:1085	The values of hygroscopicity (g adsorbed water/100 g of dry matter) indicated the lyophilized liposomes were able to decrease the capacity of the agglomerated cornstarch to absorb water, decreasing the possibility of microbiological contamination.
29023716	3	56	from	processes	377:385	arg1	equipment					401:409	high-shear equipment	390:409	high-shear equipment	390:409	The wet-agglomeration processes in high-shear equipment are useful to produce granules from binary mixtures, obtaining a powder with homogeneous composition and without segregation or elutriation of fine particles.
29023716	10	57	theme	low	1843:1845	arg1	levels					1847:1852	low levels	1843:1852	low levels of retrogradation of granules for their stability	1843:1902	Agglomeration of cornstarch with more than 8% (w/w) lyophilized liposomes decreased the tendency of starch to retrograde, which is very interesting for food products which requires low levels of retrogradation of granules for their stability.
29023716	6	58	theme	similar	976:982	arg1	values					984:989	similar values	976:989	similar values ranging from 4.42% to 4.57%	976:1017	The moisture of all formulations showed similar values ranging from 4.42% to 4.57%.
29023716	8	59	theme	quercetin-loaded	1284:1299	arg1	liposomes					1313:1321	quercetin-loaded lyophilized liposomes	1284:1321	quercetin-loaded lyophilized liposomes	1284:1321	The addition of quercetin-loaded lyophilized liposomes improved the flowability and turned the powder (agglomerated cornstarch) less cohesive.
29023716	7	60	theme	g	1050:1050	arg1	g					1071:1071	g adsorbed water/100 g	1050:1071	g adsorbed water/100 g of dry matter	1050:1085	The values of hygroscopicity (g adsorbed water/100 g of dry matter) indicated the lyophilized liposomes were able to decrease the capacity of the agglomerated cornstarch to absorb water, decreasing the possibility of microbiological contamination.
29023716	4	61	contain	containing	605:614	arg2	microparticles					616:629	microparticles	616:629	microparticles of native cornstarch	616:650	In this study, the binary mixtures containing microparticles of native cornstarch and nanoparticles of quercetin liposomes were aggregated in high-shear batches, using maltodextrin solution as binder agent.
29023716	4	61	contain	containing	605:614	arg1	mixtures					596:603	the binary mixtures	585:603	the binary mixtures containing microparticles of native cornstarch	585:650	In this study, the binary mixtures containing microparticles of native cornstarch and nanoparticles of quercetin liposomes were aggregated in high-shear batches, using maltodextrin solution as binder agent.
29023716	6	62	theme	formulations	956:967	arg1	moisture					940:947	The moisture	936:947	The moisture of all formulations	936:967	The moisture of all formulations showed similar values ranging from 4.42% to 4.57%.
29023716	4	63	theme	binder	763:768	arg1	agent					770:774	binder agent	763:774	binder agent	763:774	In this study, the binary mixtures containing microparticles of native cornstarch and nanoparticles of quercetin liposomes were aggregated in high-shear batches, using maltodextrin solution as binder agent.
29023716	7	64	theme	hygroscopicity	1034:1047	arg1	values					1024:1029	The values	1020:1029	The values of hygroscopicity (g adsorbed water/100 g of dry matter)	1020:1086	The values of hygroscopicity (g adsorbed water/100 g of dry matter) indicated the lyophilized liposomes were able to decrease the capacity of the agglomerated cornstarch to absorb water, decreasing the possibility of microbiological contamination.
29023716	9	65	from	difference	1628:1637	arg1	viscosity					1651:1659	the peak viscosity	1642:1659	the peak viscosity	1642:1659	The pasting properties of enriched agglomerated cornstarch decreased the pasting temperature about 10 °C, and the cornstarch agglomerated with 8% (w/w) of quercetin-loaded lyophilized liposomes showed no significance difference in the peak viscosity.
29023716	12	66	used	used	2114:2117	arg2	process					2102:2108	The agglomeration process	2084:2108	The agglomeration process	2084:2108	The agglomeration process was used to obtain granules of cornstarch, an ingredient extremely used in the food industry, enriched with quercetin-loaded lyophilized liposomes.
29023716	5	67	theme	physical	900:907	arg1	properties					909:918	the physical properties	896:918	the physical properties	896:918	The cornstarch was enriched by agglomeration with 8%, 22%, and 30% (w/w) of quercetin-loaded lyophilized liposomes and the physical properties were evaluated.
29023716	12	68	theme	agglomeration	2088:2100	arg1	process					2102:2108	The agglomeration process	2084:2108	The agglomeration process	2084:2108	The agglomeration process was used to obtain granules of cornstarch, an ingredient extremely used in the food industry, enriched with quercetin-loaded lyophilized liposomes.
29023716	0	69	theme	Particulate	86:96	arg1	Mixtures					105:112	Particulate Binary Mixtures	86:112	Particulate Binary Mixtures Using High Shear Process	86:137	Production of Cornstarch Granules Enriched with Quercetin Liposomes by Aggregation of Particulate Binary Mixtures Using High Shear Process.
29023716	13	70	theme	quality	2313:2319	arg1	goals					2262:2266	The goals	2258:2266	The goals	2258:2266	The goals were both the improvement of the nutritional quality and the increase of the added value of the cornstarch.
29023716	13	70	theme	quality	2313:2319	arg1	increase					2329:2336	the increase	2325:2336	the increase of the added value of the cornstarch	2325:2373	The goals were both the improvement of the nutritional quality and the increase of the added value of the cornstarch.
29023716	13	70	theme	quality	2313:2319	arg1	improvement					2282:2292	the improvement	2278:2292	the improvement of the nutritional quality	2278:2319	The goals were both the improvement of the nutritional quality and the increase of the added value of the cornstarch.
29023716	0	71	theme	Shear	125:129	arg1	Process					131:137	High Shear Process	120:137	High Shear Process	120:137	Production of Cornstarch Granules Enriched with Quercetin Liposomes by Aggregation of Particulate Binary Mixtures Using High Shear Process.
29023716	0	72	theme	Mixtures	105:112	arg1	Aggregation					71:81	Aggregation	71:81	Aggregation of Particulate Binary Mixtures Using High Shear Process	71:137	Production of Cornstarch Granules Enriched with Quercetin Liposomes by Aggregation of Particulate Binary Mixtures Using High Shear Process.
29023716	3	73	theme	high-shear	390:399	arg1	equipment					401:409	high-shear equipment	390:409	high-shear equipment	390:409	The wet-agglomeration processes in high-shear equipment are useful to produce granules from binary mixtures, obtaining a powder with homogeneous composition and without segregation or elutriation of fine particles.
29023716	0	74	theme	High	120:123	arg1	Process					131:137	High Shear Process	120:137	High Shear Process	120:137	Production of Cornstarch Granules Enriched with Quercetin Liposomes by Aggregation of Particulate Binary Mixtures Using High Shear Process.
29023716	12	75	theme	cornstarch	2141:2150	arg1	granules					2129:2136	granules	2129:2136	granules of cornstarch, an ingredient extremely used in the food industry, enriched with quercetin-loaded lyophilized liposomes	2129:2255	The agglomeration process was used to obtain granules of cornstarch, an ingredient extremely used in the food industry, enriched with quercetin-loaded lyophilized liposomes.
29023716	9	76	theme	lyophilized	1583:1593	arg1	liposomes					1595:1603	quercetin-loaded lyophilized liposomes	1566:1603	quercetin-loaded lyophilized liposomes	1566:1603	The pasting properties of enriched agglomerated cornstarch decreased the pasting temperature about 10 °C, and the cornstarch agglomerated with 8% (w/w) of quercetin-loaded lyophilized liposomes showed no significance difference in the peak viscosity.
29023716	11	77	theme	unprecedent	1944:1954	arg1	study					1932:1936	This study	1927:1936	This study	1927:1936	PRACTICAL APPLICATION This study is an unprecedent association of 2 technologies, nanoencapsulation and wet agglomeration, here used together to enrich cornstarch with quercetin.
29023716	11	77	theme	unprecedent	1944:1954	arg1	association					1956:1966	an unprecedent association	1941:1966	an unprecedent association of 2 technologies, nanoencapsulation and wet agglomeration,	1941:2026	PRACTICAL APPLICATION This study is an unprecedent association of 2 technologies, nanoencapsulation and wet agglomeration, here used together to enrich cornstarch with quercetin.
29023716	7	78	theme	agglomerated	1166:1177	arg1	cornstarch					1179:1188	the agglomerated cornstarch	1162:1188	the agglomerated cornstarch	1162:1188	The values of hygroscopicity (g adsorbed water/100 g of dry matter) indicated the lyophilized liposomes were able to decrease the capacity of the agglomerated cornstarch to absorb water, decreasing the possibility of microbiological contamination.
29023716	0	79	theme	Granules	25:32	arg1	Production					0:9	Production	0:9	Production of Cornstarch Granules	0:32	Production of Cornstarch Granules Enriched with Quercetin Liposomes by Aggregation of Particulate Binary Mixtures Using High Shear Process.
29023716	9	80	theme	significance	1615:1626	arg1	difference					1628:1637	no significance difference	1612:1637	no significance difference in the peak viscosity	1612:1659	The pasting properties of enriched agglomerated cornstarch decreased the pasting temperature about 10 °C, and the cornstarch agglomerated with 8% (w/w) of quercetin-loaded lyophilized liposomes showed no significance difference in the peak viscosity.
29023716	11	81	theme	technologies	1973:1984	arg1	study					1932:1936	This study	1927:1936	This study	1927:1936	PRACTICAL APPLICATION This study is an unprecedent association of 2 technologies, nanoencapsulation and wet agglomeration, here used together to enrich cornstarch with quercetin.
29023716	11	81	theme	technologies	1973:1984	arg1	association					1956:1966	an unprecedent association	1941:1966	an unprecedent association of 2 technologies, nanoencapsulation and wet agglomeration,	1941:2026	PRACTICAL APPLICATION This study is an unprecedent association of 2 technologies, nanoencapsulation and wet agglomeration, here used together to enrich cornstarch with quercetin.
29023716	3	82	theme	binary	447:452	arg1	mixtures					454:461	binary mixtures	447:461	binary mixtures	447:461	The wet-agglomeration processes in high-shear equipment are useful to produce granules from binary mixtures, obtaining a powder with homogeneous composition and without segregation or elutriation of fine particles.
29023716	10	83	theme	%	1706:1706	arg1	liposomes					1726:1734	more than 8% (w/w) lyophilized liposomes	1695:1734	more than 8% (w/w) lyophilized liposomes	1695:1734	Agglomeration of cornstarch with more than 8% (w/w) lyophilized liposomes decreased the tendency of starch to retrograde, which is very interesting for food products which requires low levels of retrogradation of granules for their stability.
29023716	9	84	theme	peak	1646:1649	arg1	viscosity					1651:1659	the peak viscosity	1642:1659	the peak viscosity	1642:1659	The pasting properties of enriched agglomerated cornstarch decreased the pasting temperature about 10 °C, and the cornstarch agglomerated with 8% (w/w) of quercetin-loaded lyophilized liposomes showed no significance difference in the peak viscosity.
29023716	12	85	theme	lyophilized	2235:2245	arg1	liposomes					2247:2255	quercetin-loaded lyophilized liposomes	2218:2255	quercetin-loaded lyophilized liposomes	2218:2255	The agglomeration process was used to obtain granules of cornstarch, an ingredient extremely used in the food industry, enriched with quercetin-loaded lyophilized liposomes.
29023716	12	86	theme	food	2189:2192	arg1	industry					2194:2201	the food industry	2185:2201	the food industry	2185:2201	The agglomeration process was used to obtain granules of cornstarch, an ingredient extremely used in the food industry, enriched with quercetin-loaded lyophilized liposomes.
29023716	3	87	from	mixtures	454:461	arg1	granules					433:440	granules	433:440	granules from binary mixtures	433:461	The wet-agglomeration processes in high-shear equipment are useful to produce granules from binary mixtures, obtaining a powder with homogeneous composition and without segregation or elutriation of fine particles.
29023716	10	88	theme	lyophilized	1714:1724	arg1	liposomes					1726:1734	more than 8% (w/w) lyophilized liposomes	1695:1734	more than 8% (w/w) lyophilized liposomes	1695:1734	Agglomeration of cornstarch with more than 8% (w/w) lyophilized liposomes decreased the tendency of starch to retrograde, which is very interesting for food products which requires low levels of retrogradation of granules for their stability.
29023716	5	89	theme	quercetin-loaded	853:868	arg1	liposomes					882:890	quercetin-loaded lyophilized liposomes	853:890	quercetin-loaded lyophilized liposomes	853:890	The cornstarch was enriched by agglomeration with 8%, 22%, and 30% (w/w) of quercetin-loaded lyophilized liposomes and the physical properties were evaluated.
29023716	10	90	theme	starch	1762:1767	arg1	tendency					1750:1757	the tendency	1746:1757	the tendency of starch to retrograde, which is very interesting for food products which requires low levels of retrogradation of granules for their stability	1746:1902	Agglomeration of cornstarch with more than 8% (w/w) lyophilized liposomes decreased the tendency of starch to retrograde, which is very interesting for food products which requires low levels of retrogradation of granules for their stability.
29023716	9	91	theme	enriched	1437:1444	arg1	cornstarch					1459:1468	enriched agglomerated cornstarch	1437:1468	enriched agglomerated cornstarch	1437:1468	The pasting properties of enriched agglomerated cornstarch decreased the pasting temperature about 10 °C, and the cornstarch agglomerated with 8% (w/w) of quercetin-loaded lyophilized liposomes showed no significance difference in the peak viscosity.
28917898	1	0	theme	assisted	343:350	arg1	extraction					352:361	cellulose-enzyme assisted extraction	326:361	cellulose-enzyme assisted extraction (CAE)	326:367	Polysaccharides MP-A, MP-U and MP-C were extracted from Mesona chinensis Benth by hot-alkali extraction (HAE), ultrasonic-assisted extraction (UAE) and cellulose-enzyme assisted extraction (CAE), respectively.
28917898	1	0	theme	assisted	343:350	arg1	CAE					364:366	CAE	364:366	CAE	364:366	Polysaccharides MP-A, MP-U and MP-C were extracted from Mesona chinensis Benth by hot-alkali extraction (HAE), ultrasonic-assisted extraction (UAE) and cellulose-enzyme assisted extraction (CAE), respectively.
28917898	6	1	theme	slight	1038:1043	arg1	effects					1045:1051	only slight effects	1033:1051	only slight effects	1033:1051	The three extraction methods had only slight effects on chemical composition, while MP-C extracted by CAE exhibited the highest antioxidant activity, which could potentially be used for an addition ingredient as it was previously shown to have good gelling property in food products.
28917898	6	2	contain	have	1239:1242	arg2	property					1257:1264	good gelling property	1244:1264	good gelling property	1244:1264	The three extraction methods had only slight effects on chemical composition, while MP-C extracted by CAE exhibited the highest antioxidant activity, which could potentially be used for an addition ingredient as it was previously shown to have good gelling property in food products.
28917898	6	2	contain	have	1239:1242	arg1	it					1212:1213	it	1212:1213	it	1212:1213	The three extraction methods had only slight effects on chemical composition, while MP-C extracted by CAE exhibited the highest antioxidant activity, which could potentially be used for an addition ingredient as it was previously shown to have good gelling property in food products.
28917898	1	3	theme	hot-alkali	256:265	arg1	extraction					267:276	hot-alkali extraction	256:276	hot-alkali extraction (HAE)	256:282	Polysaccharides MP-A, MP-U and MP-C were extracted from Mesona chinensis Benth by hot-alkali extraction (HAE), ultrasonic-assisted extraction (UAE) and cellulose-enzyme assisted extraction (CAE), respectively.
28917898	1	3	theme	hot-alkali	256:265	arg1	HAE					279:281	HAE	279:281	HAE	279:281	Polysaccharides MP-A, MP-U and MP-C were extracted from Mesona chinensis Benth by hot-alkali extraction (HAE), ultrasonic-assisted extraction (UAE) and cellulose-enzyme assisted extraction (CAE), respectively.
28917898	6	4	theme	food	1269:1272	arg1	products					1274:1281	food products	1269:1281	food products	1269:1281	The three extraction methods had only slight effects on chemical composition, while MP-C extracted by CAE exhibited the highest antioxidant activity, which could potentially be used for an addition ingredient as it was previously shown to have good gelling property in food products.
28917898	0	5	from	characterization	16:31	arg1	Benth					96:100	Benth	96:100	Benth	96:100	Physicochemical characterization, antioxidant activity of polysaccharides from Mesona chinensis Benth and their protective effect on injured NCTC-1469 cells induced by H2O2.
28917898	0	5	from	characterization	16:31	arg1	cells					151:155	injured NCTC-1469 cells	133:155	injured NCTC-1469 cells induced by H2O2	133:171	Physicochemical characterization, antioxidant activity of polysaccharides from Mesona chinensis Benth and their protective effect on injured NCTC-1469 cells induced by H2O2.
28917898	4	6	theme	1.00:1.34:0.25	794:807	arg1	ratios					784:789	molar ratios	778:789	molar ratios of 1.00:1.34:0.25, 1.00:2.49:0.19 and 1.00:2.95:0.84	778:842	Glucose, galactose, and galacturonic acid were the main monosaccharides in MP-A, MP-U and MP-C with molar ratios of 1.00:1.34:0.25, 1.00:2.49:0.19 and 1.00:2.95:0.84, respectively.
28917898	6	7	contain	had	1029:1031	arg1	methods					1021:1027	The three extraction methods	1000:1027	The three extraction methods	1000:1027	The three extraction methods had only slight effects on chemical composition, while MP-C extracted by CAE exhibited the highest antioxidant activity, which could potentially be used for an addition ingredient as it was previously shown to have good gelling property in food products.
28917898	6	7	contain	had	1029:1031	arg2	effects					1045:1051	only slight effects	1033:1051	only slight effects	1033:1051	The three extraction methods had only slight effects on chemical composition, while MP-C extracted by CAE exhibited the highest antioxidant activity, which could potentially be used for an addition ingredient as it was previously shown to have good gelling property in food products.
28917898	0	8	theme	protective	112:121	arg1	effect					123:128	their protective effect	106:128	their protective effect on injured NCTC-1469 cells induced by H2O2	106:171	Physicochemical characterization, antioxidant activity of polysaccharides from Mesona chinensis Benth and their protective effect on injured NCTC-1469 cells induced by H2O2.
28917898	3	9	theme	1.9×105Da	645:653	arg1	weights					634:640	average molecular weights	616:640	average molecular weights of 1.9×105Da, 1.5×105Da, 1.4×105Da	616:675	Results showed that the yields of MP-A (11.14%), MP-U (10.62%) and MP-C (9.70%) were similar, and they were all heteropolysaccharides with average molecular weights of 1.9×105Da, 1.5×105Da, 1.4×105Da.
28917898	6	10	theme	antioxidant	1128:1138	arg1	activity					1140:1147	the highest antioxidant activity	1116:1147	the highest antioxidant activity	1116:1147	The three extraction methods had only slight effects on chemical composition, while MP-C extracted by CAE exhibited the highest antioxidant activity, which could potentially be used for an addition ingredient as it was previously shown to have good gelling property in food products.
28917898	5	11	theme	hydroxyl	936:943	arg1	assays					953:958	hydroxyl radical assays	936:958	hydroxyl radical assays	936:958	MP-C extracted by CAE exhibited higher antioxidant activities in FRAP, DPPH, hydroxyl radical assays and the H2O2-induced injury cell model.
28917898	4	12	with	monosaccharides	734:748	arg1	ratios					784:789	molar ratios	778:789	molar ratios of 1.00:1.34:0.25, 1.00:2.49:0.19 and 1.00:2.95:0.84	778:842	Glucose, galactose, and galacturonic acid were the main monosaccharides in MP-A, MP-U and MP-C with molar ratios of 1.00:1.34:0.25, 1.00:2.49:0.19 and 1.00:2.95:0.84, respectively.
28917898	5	13	theme	higher	891:896	arg1	activities					910:919	higher antioxidant activities	891:919	higher antioxidant activities	891:919	MP-C extracted by CAE exhibited higher antioxidant activities in FRAP, DPPH, hydroxyl radical assays and the H2O2-induced injury cell model.
28917898	4	14	theme	1.00:2.49:0.19	810:823	arg1	ratios					784:789	molar ratios	778:789	molar ratios of 1.00:1.34:0.25, 1.00:2.49:0.19 and 1.00:2.95:0.84	778:842	Glucose, galactose, and galacturonic acid were the main monosaccharides in MP-A, MP-U and MP-C with molar ratios of 1.00:1.34:0.25, 1.00:2.49:0.19 and 1.00:2.95:0.84, respectively.
28917898	4	15	theme	galacturonic	702:713	arg1	acid					715:718	galacturonic acid	702:718	galacturonic acid	702:718	Glucose, galactose, and galacturonic acid were the main monosaccharides in MP-A, MP-U and MP-C with molar ratios of 1.00:1.34:0.25, 1.00:2.49:0.19 and 1.00:2.95:0.84, respectively.
28917898	4	15	theme	galacturonic	702:713	arg1	galactose					687:695	galactose	687:695	galactose	687:695	Glucose, galactose, and galacturonic acid were the main monosaccharides in MP-A, MP-U and MP-C with molar ratios of 1.00:1.34:0.25, 1.00:2.49:0.19 and 1.00:2.95:0.84, respectively.
28917898	4	15	theme	galacturonic	702:713	arg1	monosaccharides					734:748	the main monosaccharides	725:748	the main monosaccharides in MP-A, MP-U and MP-C	725:771	Glucose, galactose, and galacturonic acid were the main monosaccharides in MP-A, MP-U and MP-C with molar ratios of 1.00:1.34:0.25, 1.00:2.49:0.19 and 1.00:2.95:0.84, respectively.
28917898	4	15	theme	galacturonic	702:713	arg1	Glucose					678:684	Glucose	678:684	Glucose	678:684	Glucose, galactose, and galacturonic acid were the main monosaccharides in MP-A, MP-U and MP-C with molar ratios of 1.00:1.34:0.25, 1.00:2.49:0.19 and 1.00:2.95:0.84, respectively.
28917898	4	16	theme	molar	778:782	arg1	ratios					784:789	molar ratios	778:789	molar ratios of 1.00:1.34:0.25, 1.00:2.49:0.19 and 1.00:2.95:0.84	778:842	Glucose, galactose, and galacturonic acid were the main monosaccharides in MP-A, MP-U and MP-C with molar ratios of 1.00:1.34:0.25, 1.00:2.49:0.19 and 1.00:2.95:0.84, respectively.
28917898	3	17	theme	MP-U	526:529	arg1	similar					562:568	similar	562:568	similar	562:568	Results showed that the yields of MP-A (11.14%), MP-U (10.62%) and MP-C (9.70%) were similar, and they were all heteropolysaccharides with average molecular weights of 1.9×105Da, 1.5×105Da, 1.4×105Da.
28917898	3	17	theme	MP-U	526:529	arg1	yields					501:506	the yields	497:506	the yields of MP-A (11.14%), MP-U (10.62%) and MP-C (9.70%)	497:555	Results showed that the yields of MP-A (11.14%), MP-U (10.62%) and MP-C (9.70%) were similar, and they were all heteropolysaccharides with average molecular weights of 1.9×105Da, 1.5×105Da, 1.4×105Da.
28917898	0	18	from	activity	46:53	arg1	Benth					96:100	Benth	96:100	Benth	96:100	Physicochemical characterization, antioxidant activity of polysaccharides from Mesona chinensis Benth and their protective effect on injured NCTC-1469 cells induced by H2O2.
28917898	0	18	from	activity	46:53	arg1	cells					151:155	injured NCTC-1469 cells	133:155	injured NCTC-1469 cells induced by H2O2	133:171	Physicochemical characterization, antioxidant activity of polysaccharides from Mesona chinensis Benth and their protective effect on injured NCTC-1469 cells induced by H2O2.
28917898	6	19	theme	addition	1189:1196	arg1	ingredient					1198:1207	an addition ingredient	1186:1207	an addition ingredient	1186:1207	The three extraction methods had only slight effects on chemical composition, while MP-C extracted by CAE exhibited the highest antioxidant activity, which could potentially be used for an addition ingredient as it was previously shown to have good gelling property in food products.
28917898	6	20	theme	good	1244:1247	arg1	property					1257:1264	good gelling property	1244:1264	good gelling property	1244:1264	The three extraction methods had only slight effects on chemical composition, while MP-C extracted by CAE exhibited the highest antioxidant activity, which could potentially be used for an addition ingredient as it was previously shown to have good gelling property in food products.
28917898	6	21	theme	extraction	1010:1019	arg1	methods					1021:1027	The three extraction methods	1000:1027	The three extraction methods	1000:1027	The three extraction methods had only slight effects on chemical composition, while MP-C extracted by CAE exhibited the highest antioxidant activity, which could potentially be used for an addition ingredient as it was previously shown to have good gelling property in food products.
28917898	0	22	theme	Physicochemical	0:14	arg1	characterization					16:31	Physicochemical characterization	0:31	Physicochemical characterization	0:31	Physicochemical characterization, antioxidant activity of polysaccharides from Mesona chinensis Benth and their protective effect on injured NCTC-1469 cells induced by H2O2.
28917898	1	23	dep	Polysaccharides	174:188	arg1	MP-U					196:199	MP-U	196:199	MP-U	196:199	Polysaccharides MP-A, MP-U and MP-C were extracted from Mesona chinensis Benth by hot-alkali extraction (HAE), ultrasonic-assisted extraction (UAE) and cellulose-enzyme assisted extraction (CAE), respectively.
28917898	1	23	dep	Polysaccharides	174:188	arg1	MP-A					190:193	MP-A	190:193	MP-A	190:193	Polysaccharides MP-A, MP-U and MP-C were extracted from Mesona chinensis Benth by hot-alkali extraction (HAE), ultrasonic-assisted extraction (UAE) and cellulose-enzyme assisted extraction (CAE), respectively.
28917898	1	23	dep	Polysaccharides	174:188	arg1	MP-C					205:208	MP-C	205:208	MP-C	205:208	Polysaccharides MP-A, MP-U and MP-C were extracted from Mesona chinensis Benth by hot-alkali extraction (HAE), ultrasonic-assisted extraction (UAE) and cellulose-enzyme assisted extraction (CAE), respectively.
28917898	1	23	dep	Polysaccharides	174:188	arg1	Polysaccharides					174:188	Polysaccharides MP-A, MP-U and MP-C	174:208	Polysaccharides MP-A, MP-U and MP-C	174:208	Polysaccharides MP-A, MP-U and MP-C were extracted from Mesona chinensis Benth by hot-alkali extraction (HAE), ultrasonic-assisted extraction (UAE) and cellulose-enzyme assisted extraction (CAE), respectively.
28917898	2	24	theme	polysaccharides	442:456	arg1	yields					388:393	The yields	384:393	The yields	384:393	The yields, physicochemical properties and antioxidant of polysaccharides were investigated.
28917898	2	24	theme	polysaccharides	442:456	arg1	properties					412:421	physicochemical properties	396:421	physicochemical properties	396:421	The yields, physicochemical properties and antioxidant of polysaccharides were investigated.
28917898	2	24	theme	polysaccharides	442:456	arg1	antioxidant					427:437	antioxidant	427:437	antioxidant of polysaccharides	427:456	The yields, physicochemical properties and antioxidant of polysaccharides were investigated.
28917898	0	25	theme	NCTC-1469	141:149	arg1	cells					151:155	injured NCTC-1469 cells	133:155	injured NCTC-1469 cells induced by H2O2	133:171	Physicochemical characterization, antioxidant activity of polysaccharides from Mesona chinensis Benth and their protective effect on injured NCTC-1469 cells induced by H2O2.
28917898	5	26	theme	cell	988:991	arg1	model					993:997	the H2O2-induced injury cell model	964:997	the H2O2-induced injury cell model	964:997	MP-C extracted by CAE exhibited higher antioxidant activities in FRAP, DPPH, hydroxyl radical assays and the H2O2-induced injury cell model.
28917898	3	27	theme	MP-C	544:547	arg1	similar					562:568	similar	562:568	similar	562:568	Results showed that the yields of MP-A (11.14%), MP-U (10.62%) and MP-C (9.70%) were similar, and they were all heteropolysaccharides with average molecular weights of 1.9×105Da, 1.5×105Da, 1.4×105Da.
28917898	3	27	theme	MP-C	544:547	arg1	yields					501:506	the yields	497:506	the yields of MP-A (11.14%), MP-U (10.62%) and MP-C (9.70%)	497:555	Results showed that the yields of MP-A (11.14%), MP-U (10.62%) and MP-C (9.70%) were similar, and they were all heteropolysaccharides with average molecular weights of 1.9×105Da, 1.5×105Da, 1.4×105Da.
28917898	1	28	theme	ultrasonic-assisted	285:303	arg1	UAE					317:319	UAE	317:319	UAE	317:319	Polysaccharides MP-A, MP-U and MP-C were extracted from Mesona chinensis Benth by hot-alkali extraction (HAE), ultrasonic-assisted extraction (UAE) and cellulose-enzyme assisted extraction (CAE), respectively.
28917898	1	28	theme	ultrasonic-assisted	285:303	arg1	extraction					305:314	ultrasonic-assisted extraction	285:314	ultrasonic-assisted extraction (UAE)	285:320	Polysaccharides MP-A, MP-U and MP-C were extracted from Mesona chinensis Benth by hot-alkali extraction (HAE), ultrasonic-assisted extraction (UAE) and cellulose-enzyme assisted extraction (CAE), respectively.
28917898	0	29	theme	injured	133:139	arg1	cells					151:155	injured NCTC-1469 cells	133:155	injured NCTC-1469 cells induced by H2O2	133:171	Physicochemical characterization, antioxidant activity of polysaccharides from Mesona chinensis Benth and their protective effect on injured NCTC-1469 cells induced by H2O2.
28917898	0	30	from	Benth	96:100	arg1	polysaccharides					58:72	polysaccharides	58:72	polysaccharides from Mesona chinensis Benth	58:100	Physicochemical characterization, antioxidant activity of polysaccharides from Mesona chinensis Benth and their protective effect on injured NCTC-1469 cells induced by H2O2.
28917898	0	30	from	Benth	96:100	arg1	effect					123:128	their protective effect	106:128	their protective effect on injured NCTC-1469 cells induced by H2O2	106:171	Physicochemical characterization, antioxidant activity of polysaccharides from Mesona chinensis Benth and their protective effect on injured NCTC-1469 cells induced by H2O2.
28917898	0	30	from	Benth	96:100	arg1	characterization					16:31	Physicochemical characterization	0:31	Physicochemical characterization	0:31	Physicochemical characterization, antioxidant activity of polysaccharides from Mesona chinensis Benth and their protective effect on injured NCTC-1469 cells induced by H2O2.
28917898	0	30	from	Benth	96:100	arg1	activity					46:53	antioxidant activity	34:53	antioxidant activity of polysaccharides from Mesona chinensis Benth	34:100	Physicochemical characterization, antioxidant activity of polysaccharides from Mesona chinensis Benth and their protective effect on injured NCTC-1469 cells induced by H2O2.
28917898	3	31	with	heteropolysaccharides	589:609	arg1	weights					634:640	average molecular weights	616:640	average molecular weights of 1.9×105Da, 1.5×105Da, 1.4×105Da	616:675	Results showed that the yields of MP-A (11.14%), MP-U (10.62%) and MP-C (9.70%) were similar, and they were all heteropolysaccharides with average molecular weights of 1.9×105Da, 1.5×105Da, 1.4×105Da.
28917898	0	32	theme	antioxidant	34:44	arg1	activity					46:53	antioxidant activity	34:53	antioxidant activity of polysaccharides from Mesona chinensis Benth	34:100	Physicochemical characterization, antioxidant activity of polysaccharides from Mesona chinensis Benth and their protective effect on injured NCTC-1469 cells induced by H2O2.
28917898	2	33	theme	physicochemical	396:410	arg1	properties					412:421	physicochemical properties	396:421	physicochemical properties	396:421	The yields, physicochemical properties and antioxidant of polysaccharides were investigated.
28917898	6	34	theme	gelling	1249:1255	arg1	property					1257:1264	good gelling property	1244:1264	good gelling property	1244:1264	The three extraction methods had only slight effects on chemical composition, while MP-C extracted by CAE exhibited the highest antioxidant activity, which could potentially be used for an addition ingredient as it was previously shown to have good gelling property in food products.
28917898	5	35	theme	H2O2-induced	968:979	arg1	model					993:997	the H2O2-induced injury cell model	964:997	the H2O2-induced injury cell model	964:997	MP-C extracted by CAE exhibited higher antioxidant activities in FRAP, DPPH, hydroxyl radical assays and the H2O2-induced injury cell model.
28917898	0	36	theme	polysaccharides	58:72	arg1	effect					123:128	their protective effect	106:128	their protective effect on injured NCTC-1469 cells induced by H2O2	106:171	Physicochemical characterization, antioxidant activity of polysaccharides from Mesona chinensis Benth and their protective effect on injured NCTC-1469 cells induced by H2O2.
28917898	0	36	theme	polysaccharides	58:72	arg1	characterization					16:31	Physicochemical characterization	0:31	Physicochemical characterization	0:31	Physicochemical characterization, antioxidant activity of polysaccharides from Mesona chinensis Benth and their protective effect on injured NCTC-1469 cells induced by H2O2.
28917898	0	36	theme	polysaccharides	58:72	arg1	activity					46:53	antioxidant activity	34:53	antioxidant activity of polysaccharides from Mesona chinensis Benth	34:100	Physicochemical characterization, antioxidant activity of polysaccharides from Mesona chinensis Benth and their protective effect on injured NCTC-1469 cells induced by H2O2.
28917898	6	37	theme	highest	1120:1126	arg1	activity					1140:1147	the highest antioxidant activity	1116:1147	the highest antioxidant activity	1116:1147	The three extraction methods had only slight effects on chemical composition, while MP-C extracted by CAE exhibited the highest antioxidant activity, which could potentially be used for an addition ingredient as it was previously shown to have good gelling property in food products.
28917898	4	38	theme	1.00:2.95:0.84	829:842	arg1	ratios					784:789	molar ratios	778:789	molar ratios of 1.00:1.34:0.25, 1.00:2.49:0.19 and 1.00:2.95:0.84	778:842	Glucose, galactose, and galacturonic acid were the main monosaccharides in MP-A, MP-U and MP-C with molar ratios of 1.00:1.34:0.25, 1.00:2.49:0.19 and 1.00:2.95:0.84, respectively.
28917898	3	39	theme	molecular	624:632	arg1	weights					634:640	average molecular weights	616:640	average molecular weights of 1.9×105Da, 1.5×105Da, 1.4×105Da	616:675	Results showed that the yields of MP-A (11.14%), MP-U (10.62%) and MP-C (9.70%) were similar, and they were all heteropolysaccharides with average molecular weights of 1.9×105Da, 1.5×105Da, 1.4×105Da.
28917898	5	40	theme	injury	981:986	arg1	model					993:997	the H2O2-induced injury cell model	964:997	the H2O2-induced injury cell model	964:997	MP-C extracted by CAE exhibited higher antioxidant activities in FRAP, DPPH, hydroxyl radical assays and the H2O2-induced injury cell model.
28917898	3	41	theme	MP-A	511:514	arg1	similar					562:568	similar	562:568	similar	562:568	Results showed that the yields of MP-A (11.14%), MP-U (10.62%) and MP-C (9.70%) were similar, and they were all heteropolysaccharides with average molecular weights of 1.9×105Da, 1.5×105Da, 1.4×105Da.
28917898	3	41	theme	MP-A	511:514	arg1	yields					501:506	the yields	497:506	the yields of MP-A (11.14%), MP-U (10.62%) and MP-C (9.70%)	497:555	Results showed that the yields of MP-A (11.14%), MP-U (10.62%) and MP-C (9.70%) were similar, and they were all heteropolysaccharides with average molecular weights of 1.9×105Da, 1.5×105Da, 1.4×105Da.
28917898	0	42	from	effect	123:128	arg1	Benth					96:100	Benth	96:100	Benth	96:100	Physicochemical characterization, antioxidant activity of polysaccharides from Mesona chinensis Benth and their protective effect on injured NCTC-1469 cells induced by H2O2.
28917898	0	42	from	effect	123:128	arg1	cells					151:155	injured NCTC-1469 cells	133:155	injured NCTC-1469 cells induced by H2O2	133:171	Physicochemical characterization, antioxidant activity of polysaccharides from Mesona chinensis Benth and their protective effect on injured NCTC-1469 cells induced by H2O2.
28917898	5	43	theme	antioxidant	898:908	arg1	activities					910:919	higher antioxidant activities	891:919	higher antioxidant activities	891:919	MP-C extracted by CAE exhibited higher antioxidant activities in FRAP, DPPH, hydroxyl radical assays and the H2O2-induced injury cell model.
28917898	4	44	theme	main	729:732	arg1	acid					715:718	galacturonic acid	702:718	galacturonic acid	702:718	Glucose, galactose, and galacturonic acid were the main monosaccharides in MP-A, MP-U and MP-C with molar ratios of 1.00:1.34:0.25, 1.00:2.49:0.19 and 1.00:2.95:0.84, respectively.
28917898	4	44	theme	main	729:732	arg1	galactose					687:695	galactose	687:695	galactose	687:695	Glucose, galactose, and galacturonic acid were the main monosaccharides in MP-A, MP-U and MP-C with molar ratios of 1.00:1.34:0.25, 1.00:2.49:0.19 and 1.00:2.95:0.84, respectively.
28917898	4	44	theme	main	729:732	arg1	monosaccharides					734:748	the main monosaccharides	725:748	the main monosaccharides in MP-A, MP-U and MP-C	725:771	Glucose, galactose, and galacturonic acid were the main monosaccharides in MP-A, MP-U and MP-C with molar ratios of 1.00:1.34:0.25, 1.00:2.49:0.19 and 1.00:2.95:0.84, respectively.
28917898	4	44	theme	main	729:732	arg1	Glucose					678:684	Glucose	678:684	Glucose	678:684	Glucose, galactose, and galacturonic acid were the main monosaccharides in MP-A, MP-U and MP-C with molar ratios of 1.00:1.34:0.25, 1.00:2.49:0.19 and 1.00:2.95:0.84, respectively.
28917898	6	45	theme	chemical	1056:1063	arg1	composition					1065:1075	chemical composition	1056:1075	chemical composition	1056:1075	The three extraction methods had only slight effects on chemical composition, while MP-C extracted by CAE exhibited the highest antioxidant activity, which could potentially be used for an addition ingredient as it was previously shown to have good gelling property in food products.
28917898	3	46	theme	average	616:622	arg1	weights					634:640	average molecular weights	616:640	average molecular weights of 1.9×105Da, 1.5×105Da, 1.4×105Da	616:675	Results showed that the yields of MP-A (11.14%), MP-U (10.62%) and MP-C (9.70%) were similar, and they were all heteropolysaccharides with average molecular weights of 1.9×105Da, 1.5×105Da, 1.4×105Da.
28917898	5	47	theme	radical	945:951	arg1	assays					953:958	hydroxyl radical assays	936:958	hydroxyl radical assays	936:958	MP-C extracted by CAE exhibited higher antioxidant activities in FRAP, DPPH, hydroxyl radical assays and the H2O2-induced injury cell model.
28917898	1	48	theme	cellulose-enzyme	326:341	arg1	extraction					352:361	cellulose-enzyme assisted extraction	326:361	cellulose-enzyme assisted extraction (CAE)	326:367	Polysaccharides MP-A, MP-U and MP-C were extracted from Mesona chinensis Benth by hot-alkali extraction (HAE), ultrasonic-assisted extraction (UAE) and cellulose-enzyme assisted extraction (CAE), respectively.
28917898	1	48	theme	cellulose-enzyme	326:341	arg1	CAE					364:366	CAE	364:366	CAE	364:366	Polysaccharides MP-A, MP-U and MP-C were extracted from Mesona chinensis Benth by hot-alkali extraction (HAE), ultrasonic-assisted extraction (UAE) and cellulose-enzyme assisted extraction (CAE), respectively.
28917898	4	49	from	monosaccharides	734:748	arg1	MP-U					759:762	MP-U	759:762	MP-U	759:762	Glucose, galactose, and galacturonic acid were the main monosaccharides in MP-A, MP-U and MP-C with molar ratios of 1.00:1.34:0.25, 1.00:2.49:0.19 and 1.00:2.95:0.84, respectively.
28917898	4	49	from	monosaccharides	734:748	arg1	MP-A					753:756	MP-A	753:756	MP-A	753:756	Glucose, galactose, and galacturonic acid were the main monosaccharides in MP-A, MP-U and MP-C with molar ratios of 1.00:1.34:0.25, 1.00:2.49:0.19 and 1.00:2.95:0.84, respectively.
28917898	4	49	from	monosaccharides	734:748	arg1	MP-C					768:771	MP-C	768:771	MP-C	768:771	Glucose, galactose, and galacturonic acid were the main monosaccharides in MP-A, MP-U and MP-C with molar ratios of 1.00:1.34:0.25, 1.00:2.49:0.19 and 1.00:2.95:0.84, respectively.
28917898	6	50	used	used	1177:1180	arg2	activity					1140:1147	the highest antioxidant activity	1116:1147	the highest antioxidant activity	1116:1147	The three extraction methods had only slight effects on chemical composition, while MP-C extracted by CAE exhibited the highest antioxidant activity, which could potentially be used for an addition ingredient as it was previously shown to have good gelling property in food products.
23729477	4	0	theme	different	804:812	arg1	strains					827:833	different francisellae strains	804:833	different francisellae strains grown in diverse bacteriologic media and within human phagocytes	804:898	The majority of lipid A of different francisellae strains grown in diverse bacteriologic media and within human phagocytes accumulated as very hydrophobic species, including free lipid A, with <10% of the lipid A molecules substituted with O-Ag polysaccharides.
23729477	3	1	with	labeling	493:500	arg1	14					525:526	14	525:526	14	525:526	We now demonstrate that metabolic labeling of francisellae with [(14)C]acetate, combined with fractionation of [(14)C]acetate-labeled lipids by ethanol precipitation rather than hot phenol-water extraction, permits a more sensitive and quantitative appraisal of overall compositional heterogeneity in lipid A and LPS.
23729477	3	1	with	labeling	493:500	arg1	acetate					530:536	[(14)C]acetate	523:536	[(14)C]acetate	523:536	We now demonstrate that metabolic labeling of francisellae with [(14)C]acetate, combined with fractionation of [(14)C]acetate-labeled lipids by ethanol precipitation rather than hot phenol-water extraction, permits a more sensitive and quantitative appraisal of overall compositional heterogeneity in lipid A and LPS.
23729477	4	2	theme	strains	827:833	arg1	A					799:799	lipid A	793:799	lipid A of different francisellae strains grown in diverse bacteriologic media and within human phagocytes	793:898	The majority of lipid A of different francisellae strains grown in diverse bacteriologic media and within human phagocytes accumulated as very hydrophobic species, including free lipid A, with <10% of the lipid A molecules substituted with O-Ag polysaccharides.
23729477	4	3	theme	A	988:988	arg1	molecules					990:998	the lipid A molecules	978:998	the lipid A molecules substituted with O-Ag polysaccharides	978:1036	The majority of lipid A of different francisellae strains grown in diverse bacteriologic media and within human phagocytes accumulated as very hydrophobic species, including free lipid A, with <10% of the lipid A molecules substituted with O-Ag polysaccharides.
23729477	6	4	theme	lipid	1436:1440	arg1	A					1442:1442	lipid A	1436:1442	lipid A	1436:1442	In summary, metabolic labeling with [(14)C]acetate greatly facilitates assessment of the effect of genotypic and/or environmental variables on the synthesis and accumulation of lipid A and LPS by Francisella, including during growth within the cytosol of infected host cells.
23729477	1	5	theme	tularensis	156:165	arg1	hallmark					132:139	A hallmark	130:139	A hallmark of Francisella tularensis	130:165	A hallmark of Francisella tularensis, a highly virulent Gram-negative bacterium, is an unusual LPS that possesses both structural heterogeneity and characteristics that may contribute to innate immune evasion.
23729477	1	5	theme	tularensis	156:165	arg1	LPS					225:227	an unusual LPS	214:227	an unusual LPS that possesses both structural heterogeneity and characteristics that may contribute to innate immune evasion	214:337	A hallmark of Francisella tularensis, a highly virulent Gram-negative bacterium, is an unusual LPS that possesses both structural heterogeneity and characteristics that may contribute to innate immune evasion.
23729477	1	5	theme	tularensis	156:165	arg1	bacterium					200:208	a highly virulent Gram-negative bacterium	168:208	a highly virulent Gram-negative bacterium	168:208	A hallmark of Francisella tularensis, a highly virulent Gram-negative bacterium, is an unusual LPS that possesses both structural heterogeneity and characteristics that may contribute to innate immune evasion.
23729477	4	6	theme	A	799:799	arg1	majority					781:788	The majority	777:788	The majority of lipid A of different francisellae strains grown in diverse bacteriologic media and within human phagocytes	777:898	The majority of lipid A of different francisellae strains grown in diverse bacteriologic media and within human phagocytes accumulated as very hydrophobic species, including free lipid A, with <10% of the lipid A molecules substituted with O-Ag polysaccharides.
23729477	1	7	theme	structural	249:258	arg1	heterogeneity					260:272	structural heterogeneity	249:272	structural heterogeneity	249:272	A hallmark of Francisella tularensis, a highly virulent Gram-negative bacterium, is an unusual LPS that possesses both structural heterogeneity and characteristics that may contribute to innate immune evasion.
23729477	3	8	theme	C	528:528	arg1	14					525:526	14	525:526	14	525:526	We now demonstrate that metabolic labeling of francisellae with [(14)C]acetate, combined with fractionation of [(14)C]acetate-labeled lipids by ethanol precipitation rather than hot phenol-water extraction, permits a more sensitive and quantitative appraisal of overall compositional heterogeneity in lipid A and LPS.
23729477	3	8	theme	C	528:528	arg1	acetate					530:536	[(14)C]acetate	523:536	[(14)C]acetate	523:536	We now demonstrate that metabolic labeling of francisellae with [(14)C]acetate, combined with fractionation of [(14)C]acetate-labeled lipids by ethanol precipitation rather than hot phenol-water extraction, permits a more sensitive and quantitative appraisal of overall compositional heterogeneity in lipid A and LPS.
23729477	1	9	theme	virulent	177:184	arg1	hallmark					132:139	A hallmark	130:139	A hallmark of Francisella tularensis	130:165	A hallmark of Francisella tularensis, a highly virulent Gram-negative bacterium, is an unusual LPS that possesses both structural heterogeneity and characteristics that may contribute to innate immune evasion.
23729477	1	9	theme	virulent	177:184	arg1	bacterium					200:208	a highly virulent Gram-negative bacterium	168:208	a highly virulent Gram-negative bacterium	168:208	A hallmark of Francisella tularensis, a highly virulent Gram-negative bacterium, is an unusual LPS that possesses both structural heterogeneity and characteristics that may contribute to innate immune evasion.
23729477	4	10	theme	human	883:887	arg1	phagocytes					889:898	human phagocytes	883:898	human phagocytes	883:898	The majority of lipid A of different francisellae strains grown in diverse bacteriologic media and within human phagocytes accumulated as very hydrophobic species, including free lipid A, with <10% of the lipid A molecules substituted with O-Ag polysaccharides.
23729477	3	11	theme	overall	721:727	arg1	heterogeneity					743:755	overall compositional heterogeneity	721:755	overall compositional heterogeneity in lipid A and LPS	721:774	We now demonstrate that metabolic labeling of francisellae with [(14)C]acetate, combined with fractionation of [(14)C]acetate-labeled lipids by ethanol precipitation rather than hot phenol-water extraction, permits a more sensitive and quantitative appraisal of overall compositional heterogeneity in lipid A and LPS.
23729477	0	12	theme	human	112:116	arg1	phagocytes					118:127	human phagocytes	112:127	human phagocytes	112:127	Metabolic labeling to characterize the overall composition of Francisella lipid A and LPS grown in broth and in human phagocytes.
23729477	6	13	with	labeling	1281:1288	arg1	acetate					1302:1308	[(14)C]acetate	1295:1308	[(14)C]acetate	1295:1308	In summary, metabolic labeling with [(14)C]acetate greatly facilitates assessment of the effect of genotypic and/or environmental variables on the synthesis and accumulation of lipid A and LPS by Francisella, including during growth within the cytosol of infected host cells.
23729477	6	13	with	labeling	1281:1288	arg1	14					1297:1298	14	1297:1298	14	1297:1298	In summary, metabolic labeling with [(14)C]acetate greatly facilitates assessment of the effect of genotypic and/or environmental variables on the synthesis and accumulation of lipid A and LPS by Francisella, including during growth within the cytosol of infected host cells.
23729477	4	14	theme	bacteriologic	852:864	arg1	media					866:870	diverse bacteriologic media	844:870	diverse bacteriologic media	844:870	The majority of lipid A of different francisellae strains grown in diverse bacteriologic media and within human phagocytes accumulated as very hydrophobic species, including free lipid A, with <10% of the lipid A molecules substituted with O-Ag polysaccharides.
23729477	3	15	theme	lipid	760:764	arg1	A					766:766	lipid A	760:766	lipid A	760:766	We now demonstrate that metabolic labeling of francisellae with [(14)C]acetate, combined with fractionation of [(14)C]acetate-labeled lipids by ethanol precipitation rather than hot phenol-water extraction, permits a more sensitive and quantitative appraisal of overall compositional heterogeneity in lipid A and LPS.
23729477	1	16	contain	possesses	234:242	arg1	hallmark					132:139	A hallmark	130:139	A hallmark of Francisella tularensis	130:165	A hallmark of Francisella tularensis, a highly virulent Gram-negative bacterium, is an unusual LPS that possesses both structural heterogeneity and characteristics that may contribute to innate immune evasion.
23729477	1	16	contain	possesses	234:242	arg2	characteristics					278:292	characteristics	278:292	characteristics that may contribute to innate immune evasion	278:337	A hallmark of Francisella tularensis, a highly virulent Gram-negative bacterium, is an unusual LPS that possesses both structural heterogeneity and characteristics that may contribute to innate immune evasion.
23729477	1	16	contain	possesses	234:242	arg2	heterogeneity					260:272	structural heterogeneity	249:272	structural heterogeneity	249:272	A hallmark of Francisella tularensis, a highly virulent Gram-negative bacterium, is an unusual LPS that possesses both structural heterogeneity and characteristics that may contribute to innate immune evasion.
23729477	1	16	contain	possesses	234:242	arg1	LPS					225:227	an unusual LPS	214:227	an unusual LPS that possesses both structural heterogeneity and characteristics that may contribute to innate immune evasion	214:337	A hallmark of Francisella tularensis, a highly virulent Gram-negative bacterium, is an unusual LPS that possesses both structural heterogeneity and characteristics that may contribute to innate immune evasion.
23729477	3	17	theme	heterogeneity	743:755	arg1	appraisal					708:716	a more sensitive and quantitative appraisal	674:716	a more sensitive and quantitative appraisal of overall compositional heterogeneity in lipid A and LPS	674:774	We now demonstrate that metabolic labeling of francisellae with [(14)C]acetate, combined with fractionation of [(14)C]acetate-labeled lipids by ethanol precipitation rather than hot phenol-water extraction, permits a more sensitive and quantitative appraisal of overall compositional heterogeneity in lipid A and LPS.
23729477	0	18	theme	Francisella	62:72	arg1	A					80:80	Francisella lipid A	62:80	Francisella lipid A	62:80	Metabolic labeling to characterize the overall composition of Francisella lipid A and LPS grown in broth and in human phagocytes.
23729477	5	19	theme	lipid	1055:1059	arg1	A					1061:1061	lipid A	1055:1061	lipid A	1055:1061	The spectrum of lipid A and LPS species varied in a medium- and strain-dependent fashion, and growth in THP-1 cells yielded lipid A species that were not present in the same bacteria grown in brain heart infusion broth.
23729477	3	20	from	appraisal	708:716	arg1	LPS					772:774	LPS	772:774	LPS	772:774	We now demonstrate that metabolic labeling of francisellae with [(14)C]acetate, combined with fractionation of [(14)C]acetate-labeled lipids by ethanol precipitation rather than hot phenol-water extraction, permits a more sensitive and quantitative appraisal of overall compositional heterogeneity in lipid A and LPS.
23729477	3	20	from	appraisal	708:716	arg1	A					766:766	lipid A	760:766	lipid A	760:766	We now demonstrate that metabolic labeling of francisellae with [(14)C]acetate, combined with fractionation of [(14)C]acetate-labeled lipids by ethanol precipitation rather than hot phenol-water extraction, permits a more sensitive and quantitative appraisal of overall compositional heterogeneity in lipid A and LPS.
23729477	0	21	theme	A	80:80	arg1	composition					47:57	the overall composition	35:57	the overall composition of Francisella lipid A	35:80	Metabolic labeling to characterize the overall composition of Francisella lipid A and LPS grown in broth and in human phagocytes.
23729477	6	22	theme	cells	1528:1532	arg1	cytosol					1503:1509	the cytosol	1499:1509	the cytosol of infected host cells	1499:1532	In summary, metabolic labeling with [(14)C]acetate greatly facilitates assessment of the effect of genotypic and/or environmental variables on the synthesis and accumulation of lipid A and LPS by Francisella, including during growth within the cytosol of infected host cells.
23729477	6	23	theme	infected	1514:1521	arg1	cells					1528:1532	infected host cells	1514:1532	infected host cells	1514:1532	In summary, metabolic labeling with [(14)C]acetate greatly facilitates assessment of the effect of genotypic and/or environmental variables on the synthesis and accumulation of lipid A and LPS by Francisella, including during growth within the cytosol of infected host cells.
23729477	5	24	theme	heart	1237:1241	arg1	broth					1252:1256	brain heart infusion broth	1231:1256	brain heart infusion broth	1231:1256	The spectrum of lipid A and LPS species varied in a medium- and strain-dependent fashion, and growth in THP-1 cells yielded lipid A species that were not present in the same bacteria grown in brain heart infusion broth.
23729477	0	25	theme	overall	39:45	arg1	composition					47:57	the overall composition	35:57	the overall composition of Francisella lipid A	35:80	Metabolic labeling to characterize the overall composition of Francisella lipid A and LPS grown in broth and in human phagocytes.
23729477	2	26	theme	LPS	427:429	arg1	composition					431:441	the overall LPS composition	415:441	the overall LPS composition of Francisella	415:456	However, none of the methods yet employed has been sufficient to determine the overall LPS composition of Francisella.
23729477	2	27	theme	methods	361:367	arg1	none					349:352	none	349:352	none of the methods yet employed	349:380	However, none of the methods yet employed has been sufficient to determine the overall LPS composition of Francisella.
23729477	0	28	theme	Metabolic	0:8	arg1	labeling					10:17	Metabolic labeling	0:17	Metabolic labeling	0:17	Metabolic labeling to characterize the overall composition of Francisella lipid A and LPS grown in broth and in human phagocytes.
23729477	5	29	theme	medium-	1091:1097	arg1	fashion					1120:1126	a medium- and strain-dependent fashion	1089:1126	a medium- and strain-dependent fashion	1089:1126	The spectrum of lipid A and LPS species varied in a medium- and strain-dependent fashion, and growth in THP-1 cells yielded lipid A species that were not present in the same bacteria grown in brain heart infusion broth.
23729477	5	30	theme	strain-dependent	1103:1118	arg1	fashion					1120:1126	a medium- and strain-dependent fashion	1089:1126	a medium- and strain-dependent fashion	1089:1126	The spectrum of lipid A and LPS species varied in a medium- and strain-dependent fashion, and growth in THP-1 cells yielded lipid A species that were not present in the same bacteria grown in brain heart infusion broth.
23729477	4	31	theme	lipid	956:960	arg1	A					962:962	free lipid A	951:962	free lipid A	951:962	The majority of lipid A of different francisellae strains grown in diverse bacteriologic media and within human phagocytes accumulated as very hydrophobic species, including free lipid A, with <10% of the lipid A molecules substituted with O-Ag polysaccharides.
23729477	3	32	theme	acetate-labeled	577:591	arg1	lipids					593:598	[(14)C]acetate-labeled lipids	570:598	[(14)C]acetate-labeled lipids	570:598	We now demonstrate that metabolic labeling of francisellae with [(14)C]acetate, combined with fractionation of [(14)C]acetate-labeled lipids by ethanol precipitation rather than hot phenol-water extraction, permits a more sensitive and quantitative appraisal of overall compositional heterogeneity in lipid A and LPS.
23729477	3	32	theme	acetate-labeled	577:591	arg1	14					572:573	14	572:573	14	572:573	We now demonstrate that metabolic labeling of francisellae with [(14)C]acetate, combined with fractionation of [(14)C]acetate-labeled lipids by ethanol precipitation rather than hot phenol-water extraction, permits a more sensitive and quantitative appraisal of overall compositional heterogeneity in lipid A and LPS.
23729477	5	33	theme	species	1071:1077	arg1	spectrum					1043:1050	The spectrum	1039:1050	The spectrum of lipid A and LPS species	1039:1077	The spectrum of lipid A and LPS species varied in a medium- and strain-dependent fashion, and growth in THP-1 cells yielded lipid A species that were not present in the same bacteria grown in brain heart infusion broth.
23729477	2	34	theme	Francisella	446:456	arg1	composition					431:441	the overall LPS composition	415:441	the overall LPS composition of Francisella	415:456	However, none of the methods yet employed has been sufficient to determine the overall LPS composition of Francisella.
23729477	3	35	from	heterogeneity	743:755	arg1	LPS					772:774	LPS	772:774	LPS	772:774	We now demonstrate that metabolic labeling of francisellae with [(14)C]acetate, combined with fractionation of [(14)C]acetate-labeled lipids by ethanol precipitation rather than hot phenol-water extraction, permits a more sensitive and quantitative appraisal of overall compositional heterogeneity in lipid A and LPS.
23729477	3	35	from	heterogeneity	743:755	arg1	A					766:766	lipid A	760:766	lipid A	760:766	We now demonstrate that metabolic labeling of francisellae with [(14)C]acetate, combined with fractionation of [(14)C]acetate-labeled lipids by ethanol precipitation rather than hot phenol-water extraction, permits a more sensitive and quantitative appraisal of overall compositional heterogeneity in lipid A and LPS.
23729477	3	36	theme	C	575:575	arg1	lipids					593:598	[(14)C]acetate-labeled lipids	570:598	[(14)C]acetate-labeled lipids	570:598	We now demonstrate that metabolic labeling of francisellae with [(14)C]acetate, combined with fractionation of [(14)C]acetate-labeled lipids by ethanol precipitation rather than hot phenol-water extraction, permits a more sensitive and quantitative appraisal of overall compositional heterogeneity in lipid A and LPS.
23729477	3	36	theme	C	575:575	arg1	14					572:573	14	572:573	14	572:573	We now demonstrate that metabolic labeling of francisellae with [(14)C]acetate, combined with fractionation of [(14)C]acetate-labeled lipids by ethanol precipitation rather than hot phenol-water extraction, permits a more sensitive and quantitative appraisal of overall compositional heterogeneity in lipid A and LPS.
23729477	1	37	theme	innate	317:322	arg1	evasion					331:337	innate immune evasion	317:337	innate immune evasion	317:337	A hallmark of Francisella tularensis, a highly virulent Gram-negative bacterium, is an unusual LPS that possesses both structural heterogeneity and characteristics that may contribute to innate immune evasion.
23729477	5	38	theme	THP-1	1143:1147	arg1	cells					1149:1153	THP-1 cells	1143:1153	THP-1 cells	1143:1153	The spectrum of lipid A and LPS species varied in a medium- and strain-dependent fashion, and growth in THP-1 cells yielded lipid A species that were not present in the same bacteria grown in brain heart infusion broth.
23729477	6	39	from	assessment	1330:1339	arg1	accumulation					1420:1431	accumulation	1420:1431	accumulation	1420:1431	In summary, metabolic labeling with [(14)C]acetate greatly facilitates assessment of the effect of genotypic and/or environmental variables on the synthesis and accumulation of lipid A and LPS by Francisella, including during growth within the cytosol of infected host cells.
23729477	6	39	from	assessment	1330:1339	arg1	synthesis					1406:1414	synthesis	1406:1414	synthesis	1406:1414	In summary, metabolic labeling with [(14)C]acetate greatly facilitates assessment of the effect of genotypic and/or environmental variables on the synthesis and accumulation of lipid A and LPS by Francisella, including during growth within the cytosol of infected host cells.
23729477	4	40	theme	lipid	982:986	arg1	molecules					990:998	the lipid A molecules	978:998	the lipid A molecules substituted with O-Ag polysaccharides	978:1036	The majority of lipid A of different francisellae strains grown in diverse bacteriologic media and within human phagocytes accumulated as very hydrophobic species, including free lipid A, with <10% of the lipid A molecules substituted with O-Ag polysaccharides.
23729477	3	41	theme	phenol-water	641:652	arg1	extraction					654:663	hot phenol-water extraction	637:663	fractionation of [(14)C]acetate-labeled lipids by ethanol precipitation rather than hot phenol-water extraction	553:663	We now demonstrate that metabolic labeling of francisellae with [(14)C]acetate, combined with fractionation of [(14)C]acetate-labeled lipids by ethanol precipitation rather than hot phenol-water extraction, permits a more sensitive and quantitative appraisal of overall compositional heterogeneity in lipid A and LPS.
23729477	5	42	attach	present	1193:1199	arg1	bacteria					1213:1220	the same bacteria	1204:1220	the same bacteria grown in brain heart infusion broth	1204:1256	The spectrum of lipid A and LPS species varied in a medium- and strain-dependent fashion, and growth in THP-1 cells yielded lipid A species that were not present in the same bacteria grown in brain heart infusion broth.
23729477	5	42	attach	present	1193:1199	arg2	species					1171:1177	lipid A species	1163:1177	lipid A species that were not present in the same bacteria grown in brain heart infusion broth	1163:1256	The spectrum of lipid A and LPS species varied in a medium- and strain-dependent fashion, and growth in THP-1 cells yielded lipid A species that were not present in the same bacteria grown in brain heart infusion broth.
23729477	3	43	theme	metabolic	483:491	arg1	labeling					493:500	metabolic labeling	483:500	metabolic labeling	483:500	We now demonstrate that metabolic labeling of francisellae with [(14)C]acetate, combined with fractionation of [(14)C]acetate-labeled lipids by ethanol precipitation rather than hot phenol-water extraction, permits a more sensitive and quantitative appraisal of overall compositional heterogeneity in lipid A and LPS.
23729477	5	44	theme	A	1061:1061	arg1	species					1071:1077	lipid A and LPS species	1055:1077	lipid A and LPS species	1055:1077	The spectrum of lipid A and LPS species varied in a medium- and strain-dependent fashion, and growth in THP-1 cells yielded lipid A species that were not present in the same bacteria grown in brain heart infusion broth.
23729477	4	45	theme	molecules	990:998	arg1	molecules					990:998	the lipid A molecules	978:998	the lipid A molecules substituted with O-Ag polysaccharides	978:1036	The majority of lipid A of different francisellae strains grown in diverse bacteriologic media and within human phagocytes accumulated as very hydrophobic species, including free lipid A, with <10% of the lipid A molecules substituted with O-Ag polysaccharides.
23729477	4	45	theme	molecules	990:998	arg1	%					973:973	<10%	970:973	<10% of the lipid A molecules substituted with O-Ag polysaccharides	970:1036	The majority of lipid A of different francisellae strains grown in diverse bacteriologic media and within human phagocytes accumulated as very hydrophobic species, including free lipid A, with <10% of the lipid A molecules substituted with O-Ag polysaccharides.
23729477	5	46	located	present	1193:1199	arg1	bacteria					1213:1220	the same bacteria	1204:1220	the same bacteria grown in brain heart infusion broth	1204:1256	The spectrum of lipid A and LPS species varied in a medium- and strain-dependent fashion, and growth in THP-1 cells yielded lipid A species that were not present in the same bacteria grown in brain heart infusion broth.
23729477	5	46	located	present	1193:1199	arg2	species					1171:1177	lipid A species	1163:1177	lipid A species that were not present in the same bacteria grown in brain heart infusion broth	1163:1256	The spectrum of lipid A and LPS species varied in a medium- and strain-dependent fashion, and growth in THP-1 cells yielded lipid A species that were not present in the same bacteria grown in brain heart infusion broth.
23729477	4	47	theme	francisellae	814:825	arg1	strains					827:833	different francisellae strains	804:833	different francisellae strains grown in diverse bacteriologic media and within human phagocytes	804:898	The majority of lipid A of different francisellae strains grown in diverse bacteriologic media and within human phagocytes accumulated as very hydrophobic species, including free lipid A, with <10% of the lipid A molecules substituted with O-Ag polysaccharides.
23729477	6	48	dep	synthesis	1406:1414	arg1	the					1402:1404	the	1402:1404	the	1402:1404	In summary, metabolic labeling with [(14)C]acetate greatly facilitates assessment of the effect of genotypic and/or environmental variables on the synthesis and accumulation of lipid A and LPS by Francisella, including during growth within the cytosol of infected host cells.
23729477	1	49	theme	Francisella	144:154	arg1	tularensis					156:165	Francisella tularensis	144:165	Francisella tularensis	144:165	A hallmark of Francisella tularensis, a highly virulent Gram-negative bacterium, is an unusual LPS that possesses both structural heterogeneity and characteristics that may contribute to innate immune evasion.
23729477	6	50	theme	metabolic	1271:1279	arg1	labeling					1281:1288	metabolic labeling	1271:1288	metabolic labeling with [(14)C]acetate	1271:1308	In summary, metabolic labeling with [(14)C]acetate greatly facilitates assessment of the effect of genotypic and/or environmental variables on the synthesis and accumulation of lipid A and LPS by Francisella, including during growth within the cytosol of infected host cells.
23729477	4	51	theme	lipid	793:797	arg1	A					799:799	lipid A	793:799	lipid A of different francisellae strains grown in diverse bacteriologic media and within human phagocytes	793:898	The majority of lipid A of different francisellae strains grown in diverse bacteriologic media and within human phagocytes accumulated as very hydrophobic species, including free lipid A, with <10% of the lipid A molecules substituted with O-Ag polysaccharides.
23729477	3	52	theme	quantitative	695:706	arg1	appraisal					708:716	a more sensitive and quantitative appraisal	674:716	a more sensitive and quantitative appraisal of overall compositional heterogeneity in lipid A and LPS	674:774	We now demonstrate that metabolic labeling of francisellae with [(14)C]acetate, combined with fractionation of [(14)C]acetate-labeled lipids by ethanol precipitation rather than hot phenol-water extraction, permits a more sensitive and quantitative appraisal of overall compositional heterogeneity in lipid A and LPS.
23729477	5	53	from	growth	1133:1138	arg1	cells					1149:1153	THP-1 cells	1143:1153	THP-1 cells	1143:1153	The spectrum of lipid A and LPS species varied in a medium- and strain-dependent fashion, and growth in THP-1 cells yielded lipid A species that were not present in the same bacteria grown in brain heart infusion broth.
23729477	5	54	from	present	1193:1199	arg1	bacteria					1213:1220	the same bacteria	1204:1220	the same bacteria grown in brain heart infusion broth	1204:1256	The spectrum of lipid A and LPS species varied in a medium- and strain-dependent fashion, and growth in THP-1 cells yielded lipid A species that were not present in the same bacteria grown in brain heart infusion broth.
23729477	3	55	theme	sensitive	681:689	arg1	appraisal					708:716	a more sensitive and quantitative appraisal	674:716	a more sensitive and quantitative appraisal of overall compositional heterogeneity in lipid A and LPS	674:774	We now demonstrate that metabolic labeling of francisellae with [(14)C]acetate, combined with fractionation of [(14)C]acetate-labeled lipids by ethanol precipitation rather than hot phenol-water extraction, permits a more sensitive and quantitative appraisal of overall compositional heterogeneity in lipid A and LPS.
23729477	6	56	theme	variables	1389:1397	arg1	effect					1348:1353	the effect	1344:1353	the effect of genotypic and/or environmental variables on the synthesis and accumulation of lipid A and LPS	1344:1450	In summary, metabolic labeling with [(14)C]acetate greatly facilitates assessment of the effect of genotypic and/or environmental variables on the synthesis and accumulation of lipid A and LPS by Francisella, including during growth within the cytosol of infected host cells.
23729477	1	57	theme	Gram-negative	186:198	arg1	hallmark					132:139	A hallmark	130:139	A hallmark of Francisella tularensis	130:165	A hallmark of Francisella tularensis, a highly virulent Gram-negative bacterium, is an unusual LPS that possesses both structural heterogeneity and characteristics that may contribute to innate immune evasion.
23729477	1	57	theme	Gram-negative	186:198	arg1	bacterium					200:208	a highly virulent Gram-negative bacterium	168:208	a highly virulent Gram-negative bacterium	168:208	A hallmark of Francisella tularensis, a highly virulent Gram-negative bacterium, is an unusual LPS that possesses both structural heterogeneity and characteristics that may contribute to innate immune evasion.
23729477	3	58	theme	compositional	729:741	arg1	heterogeneity					743:755	overall compositional heterogeneity	721:755	overall compositional heterogeneity in lipid A and LPS	721:774	We now demonstrate that metabolic labeling of francisellae with [(14)C]acetate, combined with fractionation of [(14)C]acetate-labeled lipids by ethanol precipitation rather than hot phenol-water extraction, permits a more sensitive and quantitative appraisal of overall compositional heterogeneity in lipid A and LPS.
23729477	5	59	from	bacteria	1213:1220	arg1	present					1193:1199	present	1193:1199	present	1193:1199	The spectrum of lipid A and LPS species varied in a medium- and strain-dependent fashion, and growth in THP-1 cells yielded lipid A species that were not present in the same bacteria grown in brain heart infusion broth.
23729477	4	60	theme	diverse	844:850	arg1	media					866:870	diverse bacteriologic media	844:870	diverse bacteriologic media	844:870	The majority of lipid A of different francisellae strains grown in diverse bacteriologic media and within human phagocytes accumulated as very hydrophobic species, including free lipid A, with <10% of the lipid A molecules substituted with O-Ag polysaccharides.
23729477	0	61	theme	lipid	74:78	arg1	A					80:80	Francisella lipid A	62:80	Francisella lipid A	62:80	Metabolic labeling to characterize the overall composition of Francisella lipid A and LPS grown in broth and in human phagocytes.
23729477	6	62	theme	LPS	1448:1450	arg1	accumulation					1420:1431	accumulation	1420:1431	accumulation	1420:1431	In summary, metabolic labeling with [(14)C]acetate greatly facilitates assessment of the effect of genotypic and/or environmental variables on the synthesis and accumulation of lipid A and LPS by Francisella, including during growth within the cytosol of infected host cells.
23729477	6	62	theme	LPS	1448:1450	arg1	synthesis					1406:1414	synthesis	1406:1414	synthesis	1406:1414	In summary, metabolic labeling with [(14)C]acetate greatly facilitates assessment of the effect of genotypic and/or environmental variables on the synthesis and accumulation of lipid A and LPS by Francisella, including during growth within the cytosol of infected host cells.
23729477	4	63	theme	hydrophobic	920:930	arg1	A					962:962	free lipid A	951:962	free lipid A	951:962	The majority of lipid A of different francisellae strains grown in diverse bacteriologic media and within human phagocytes accumulated as very hydrophobic species, including free lipid A, with <10% of the lipid A molecules substituted with O-Ag polysaccharides.
23729477	4	63	theme	hydrophobic	920:930	arg1	species					932:938	very hydrophobic species	915:938	very hydrophobic species	915:938	The majority of lipid A of different francisellae strains grown in diverse bacteriologic media and within human phagocytes accumulated as very hydrophobic species, including free lipid A, with <10% of the lipid A molecules substituted with O-Ag polysaccharides.
23729477	5	64	theme	A	1169:1169	arg1	species					1171:1177	lipid A species	1163:1177	lipid A species that were not present in the same bacteria grown in brain heart infusion broth	1163:1256	The spectrum of lipid A and LPS species varied in a medium- and strain-dependent fashion, and growth in THP-1 cells yielded lipid A species that were not present in the same bacteria grown in brain heart infusion broth.
23729477	1	65	theme	immune	324:329	arg1	evasion					331:337	innate immune evasion	317:337	innate immune evasion	317:337	A hallmark of Francisella tularensis, a highly virulent Gram-negative bacterium, is an unusual LPS that possesses both structural heterogeneity and characteristics that may contribute to innate immune evasion.
23729477	6	66	theme	A	1442:1442	arg1	accumulation					1420:1431	accumulation	1420:1431	accumulation	1420:1431	In summary, metabolic labeling with [(14)C]acetate greatly facilitates assessment of the effect of genotypic and/or environmental variables on the synthesis and accumulation of lipid A and LPS by Francisella, including during growth within the cytosol of infected host cells.
23729477	6	66	theme	A	1442:1442	arg1	synthesis					1406:1414	synthesis	1406:1414	synthesis	1406:1414	In summary, metabolic labeling with [(14)C]acetate greatly facilitates assessment of the effect of genotypic and/or environmental variables on the synthesis and accumulation of lipid A and LPS by Francisella, including during growth within the cytosol of infected host cells.
23729477	5	67	theme	LPS	1067:1069	arg1	species					1071:1077	lipid A and LPS species	1055:1077	lipid A and LPS species	1055:1077	The spectrum of lipid A and LPS species varied in a medium- and strain-dependent fashion, and growth in THP-1 cells yielded lipid A species that were not present in the same bacteria grown in brain heart infusion broth.
23729477	6	68	theme	host	1523:1526	arg1	cells					1528:1532	infected host cells	1514:1532	infected host cells	1514:1532	In summary, metabolic labeling with [(14)C]acetate greatly facilitates assessment of the effect of genotypic and/or environmental variables on the synthesis and accumulation of lipid A and LPS by Francisella, including during growth within the cytosol of infected host cells.
23729477	6	69	from	accumulation	1420:1431	arg1	assessment					1330:1339	assessment	1330:1339	assessment of the effect of genotypic and/or environmental variables on the synthesis and accumulation of lipid A and LPS	1330:1450	In summary, metabolic labeling with [(14)C]acetate greatly facilitates assessment of the effect of genotypic and/or environmental variables on the synthesis and accumulation of lipid A and LPS by Francisella, including during growth within the cytosol of infected host cells.
23729477	5	70	theme	brain	1231:1235	arg1	broth					1252:1256	brain heart infusion broth	1231:1256	brain heart infusion broth	1231:1256	The spectrum of lipid A and LPS species varied in a medium- and strain-dependent fashion, and growth in THP-1 cells yielded lipid A species that were not present in the same bacteria grown in brain heart infusion broth.
23729477	5	71	theme	infusion	1243:1250	arg1	broth					1252:1256	brain heart infusion broth	1231:1256	brain heart infusion broth	1231:1256	The spectrum of lipid A and LPS species varied in a medium- and strain-dependent fashion, and growth in THP-1 cells yielded lipid A species that were not present in the same bacteria grown in brain heart infusion broth.
23729477	2	72	theme	overall	419:425	arg1	composition					431:441	the overall LPS composition	415:441	the overall LPS composition of Francisella	415:456	However, none of the methods yet employed has been sufficient to determine the overall LPS composition of Francisella.
23729477	5	73	theme	same	1208:1211	arg1	bacteria					1213:1220	the same bacteria	1204:1220	the same bacteria grown in brain heart infusion broth	1204:1256	The spectrum of lipid A and LPS species varied in a medium- and strain-dependent fashion, and growth in THP-1 cells yielded lipid A species that were not present in the same bacteria grown in brain heart infusion broth.
23729477	6	74	from	synthesis	1406:1414	arg1	assessment					1330:1339	assessment	1330:1339	assessment of the effect of genotypic and/or environmental variables on the synthesis and accumulation of lipid A and LPS	1330:1450	In summary, metabolic labeling with [(14)C]acetate greatly facilitates assessment of the effect of genotypic and/or environmental variables on the synthesis and accumulation of lipid A and LPS by Francisella, including during growth within the cytosol of infected host cells.
23729477	3	75	from	LPS	772:774	arg1	appraisal					708:716	a more sensitive and quantitative appraisal	674:716	a more sensitive and quantitative appraisal of overall compositional heterogeneity in lipid A and LPS	674:774	We now demonstrate that metabolic labeling of francisellae with [(14)C]acetate, combined with fractionation of [(14)C]acetate-labeled lipids by ethanol precipitation rather than hot phenol-water extraction, permits a more sensitive and quantitative appraisal of overall compositional heterogeneity in lipid A and LPS.
23729477	6	76	theme	C	1300:1300	arg1	acetate					1302:1308	[(14)C]acetate	1295:1308	[(14)C]acetate	1295:1308	In summary, metabolic labeling with [(14)C]acetate greatly facilitates assessment of the effect of genotypic and/or environmental variables on the synthesis and accumulation of lipid A and LPS by Francisella, including during growth within the cytosol of infected host cells.
23729477	6	76	theme	C	1300:1300	arg1	14					1297:1298	14	1297:1298	14	1297:1298	In summary, metabolic labeling with [(14)C]acetate greatly facilitates assessment of the effect of genotypic and/or environmental variables on the synthesis and accumulation of lipid A and LPS by Francisella, including during growth within the cytosol of infected host cells.
23729477	4	77	theme	free	951:954	arg1	A					962:962	free lipid A	951:962	free lipid A	951:962	The majority of lipid A of different francisellae strains grown in diverse bacteriologic media and within human phagocytes accumulated as very hydrophobic species, including free lipid A, with <10% of the lipid A molecules substituted with O-Ag polysaccharides.
23729477	3	78	theme	ethanol	603:609	arg1	precipitation					611:623	ethanol precipitation	603:623	ethanol precipitation	603:623	We now demonstrate that metabolic labeling of francisellae with [(14)C]acetate, combined with fractionation of [(14)C]acetate-labeled lipids by ethanol precipitation rather than hot phenol-water extraction, permits a more sensitive and quantitative appraisal of overall compositional heterogeneity in lipid A and LPS.
23729477	6	79	theme	environmental	1375:1387	arg1	variables					1389:1397	genotypic and/or environmental variables	1358:1397	genotypic and/or environmental variables	1358:1397	In summary, metabolic labeling with [(14)C]acetate greatly facilitates assessment of the effect of genotypic and/or environmental variables on the synthesis and accumulation of lipid A and LPS by Francisella, including during growth within the cytosol of infected host cells.
23729477	3	80	from	A	766:766	arg1	appraisal					708:716	a more sensitive and quantitative appraisal	674:716	a more sensitive and quantitative appraisal of overall compositional heterogeneity in lipid A and LPS	674:774	We now demonstrate that metabolic labeling of francisellae with [(14)C]acetate, combined with fractionation of [(14)C]acetate-labeled lipids by ethanol precipitation rather than hot phenol-water extraction, permits a more sensitive and quantitative appraisal of overall compositional heterogeneity in lipid A and LPS.
23729477	3	81	theme	lipids	593:598	arg1	fractionation					553:565	fractionation	553:565	fractionation of [(14)C]acetate-labeled lipids by ethanol precipitation rather than hot phenol-water extraction	553:663	We now demonstrate that metabolic labeling of francisellae with [(14)C]acetate, combined with fractionation of [(14)C]acetate-labeled lipids by ethanol precipitation rather than hot phenol-water extraction, permits a more sensitive and quantitative appraisal of overall compositional heterogeneity in lipid A and LPS.
23729477	6	82	theme	genotypic	1358:1366	arg1	variables					1389:1397	genotypic and/or environmental variables	1358:1397	genotypic and/or environmental variables	1358:1397	In summary, metabolic labeling with [(14)C]acetate greatly facilitates assessment of the effect of genotypic and/or environmental variables on the synthesis and accumulation of lipid A and LPS by Francisella, including during growth within the cytosol of infected host cells.
23729477	6	83	from	effect	1348:1353	arg1	accumulation					1420:1431	accumulation	1420:1431	accumulation	1420:1431	In summary, metabolic labeling with [(14)C]acetate greatly facilitates assessment of the effect of genotypic and/or environmental variables on the synthesis and accumulation of lipid A and LPS by Francisella, including during growth within the cytosol of infected host cells.
23729477	6	83	from	effect	1348:1353	arg1	synthesis					1406:1414	synthesis	1406:1414	synthesis	1406:1414	In summary, metabolic labeling with [(14)C]acetate greatly facilitates assessment of the effect of genotypic and/or environmental variables on the synthesis and accumulation of lipid A and LPS by Francisella, including during growth within the cytosol of infected host cells.
23729477	4	84	theme	O-Ag	1017:1020	arg1	polysaccharides					1022:1036	O-Ag polysaccharides	1017:1036	O-Ag polysaccharides	1017:1036	The majority of lipid A of different francisellae strains grown in diverse bacteriologic media and within human phagocytes accumulated as very hydrophobic species, including free lipid A, with <10% of the lipid A molecules substituted with O-Ag polysaccharides.
23729477	3	85	theme	hot	637:639	arg1	extraction					654:663	hot phenol-water extraction	637:663	fractionation of [(14)C]acetate-labeled lipids by ethanol precipitation rather than hot phenol-water extraction	553:663	We now demonstrate that metabolic labeling of francisellae with [(14)C]acetate, combined with fractionation of [(14)C]acetate-labeled lipids by ethanol precipitation rather than hot phenol-water extraction, permits a more sensitive and quantitative appraisal of overall compositional heterogeneity in lipid A and LPS.
23729477	1	86	theme	unusual	217:223	arg1	hallmark					132:139	A hallmark	130:139	A hallmark of Francisella tularensis	130:165	A hallmark of Francisella tularensis, a highly virulent Gram-negative bacterium, is an unusual LPS that possesses both structural heterogeneity and characteristics that may contribute to innate immune evasion.
23729477	1	86	theme	unusual	217:223	arg1	LPS					225:227	an unusual LPS	214:227	an unusual LPS that possesses both structural heterogeneity and characteristics that may contribute to innate immune evasion	214:337	A hallmark of Francisella tularensis, a highly virulent Gram-negative bacterium, is an unusual LPS that possesses both structural heterogeneity and characteristics that may contribute to innate immune evasion.
23729477	3	87	theme	francisellae	505:516	arg1	labeling					493:500	metabolic labeling	483:500	metabolic labeling	483:500	We now demonstrate that metabolic labeling of francisellae with [(14)C]acetate, combined with fractionation of [(14)C]acetate-labeled lipids by ethanol precipitation rather than hot phenol-water extraction, permits a more sensitive and quantitative appraisal of overall compositional heterogeneity in lipid A and LPS.
23729477	6	88	theme	effect	1348:1353	arg1	assessment					1330:1339	assessment	1330:1339	assessment of the effect of genotypic and/or environmental variables on the synthesis and accumulation of lipid A and LPS	1330:1450	In summary, metabolic labeling with [(14)C]acetate greatly facilitates assessment of the effect of genotypic and/or environmental variables on the synthesis and accumulation of lipid A and LPS by Francisella, including during growth within the cytosol of infected host cells.
23729477	5	89	theme	lipid	1163:1167	arg1	species					1171:1177	lipid A species	1163:1177	lipid A species that were not present in the same bacteria grown in brain heart infusion broth	1163:1256	The spectrum of lipid A and LPS species varied in a medium- and strain-dependent fashion, and growth in THP-1 cells yielded lipid A species that were not present in the same bacteria grown in brain heart infusion broth.
27752801	6	0	theme	degraded	642:649	arg1	sulfate					659:665	heterogeneous partially degraded heparan sulfate	618:665	heterogeneous partially degraded heparan sulfate	618:665	Smaller amounts (12 %) of heparan sulfate was also present which was made of more highly sulfated chains of molecular weight of 21-22 kDa and a less sulfated low molecular weight (< 10 kDa) heterogeneous partially degraded heparan sulfate.
27752801	1	1	theme	chicken	145:151	arg1	gizzard					130:136	gizzard	130:136	gizzard	130:136	Glycosaminoglycans (GAGs) were prepared from the muscular stomach or gizzard of the chicken.
27752801	1	1	theme	chicken	145:151	arg1	stomach					119:125	stomach	119:125	stomach	119:125	Glycosaminoglycans (GAGs) were prepared from the muscular stomach or gizzard of the chicken.
27752801	6	2	theme	21-22 kDa	556:564	arg1	weight					546:551	molecular weight	536:551	molecular weight of 21-22 kDa	536:564	Smaller amounts (12 %) of heparan sulfate was also present which was made of more highly sulfated chains of molecular weight of 21-22 kDa and a less sulfated low molecular weight (< 10 kDa) heterogeneous partially degraded heparan sulfate.
27752801	6	2	theme	21-22 kDa	556:564	arg1	weight					600:605	a less sulfated low molecular weight	570:605	a less sulfated low molecular weight (< 10 kDa)	570:616	Smaller amounts (12 %) of heparan sulfate was also present which was made of more highly sulfated chains of molecular weight of 21-22 kDa and a less sulfated low molecular weight (< 10 kDa) heterogeneous partially degraded heparan sulfate.
27752801	6	2	theme	21-22 kDa	556:564	arg1	10 kDa					610:615	< 10 kDa	608:615	< 10 kDa	608:615	Smaller amounts (12 %) of heparan sulfate was also present which was made of more highly sulfated chains of molecular weight of 21-22 kDa and a less sulfated low molecular weight (< 10 kDa) heterogeneous partially degraded heparan sulfate.
27752801	4	3	theme	weight	359:364	arg1	chondroitin-4-sulfate					316:336	chondroitin-4-sulfate	316:336	chondroitin-4-sulfate	316:336	The major GAG components were chondroitin-6-sulfate and chondroitin-4-sulfate (~64 %) of molecular weight 21-22 kDa.
27752801	4	3	theme	weight	359:364	arg1	%					343:343	~64 %	339:343	~64 %	339:343	The major GAG components were chondroitin-6-sulfate and chondroitin-4-sulfate (~64 %) of molecular weight 21-22 kDa.
27752801	4	3	theme	weight	359:364	arg1	chondroitin-6-sulfate					290:310	chondroitin-6-sulfate	290:310	chondroitin-6-sulfate	290:310	The major GAG components were chondroitin-6-sulfate and chondroitin-4-sulfate (~64 %) of molecular weight 21-22 kDa.
27752801	4	3	theme	weight	359:364	arg1	components					274:283	The major GAG components	260:283	The major GAG components	260:283	The major GAG components were chondroitin-6-sulfate and chondroitin-4-sulfate (~64 %) of molecular weight 21-22 kDa.
27752801	6	4	theme	weight	546:551	arg1	chains					526:531	more highly sulfated chains	505:531	more highly sulfated chains of molecular weight of 21-22 kDa and a less sulfated low molecular weight (< 10 kDa) heterogeneous partially degraded heparan sulfate	505:665	Smaller amounts (12 %) of heparan sulfate was also present which was made of more highly sulfated chains of molecular weight of 21-22 kDa and a less sulfated low molecular weight (< 10 kDa) heterogeneous partially degraded heparan sulfate.
27752801	6	5	theme	sulfate	462:468	arg1	sulfate					462:468	heparan sulfate	454:468	heparan sulfate	454:468	Smaller amounts (12 %) of heparan sulfate was also present which was made of more highly sulfated chains of molecular weight of 21-22 kDa and a less sulfated low molecular weight (< 10 kDa) heterogeneous partially degraded heparan sulfate.
27752801	6	5	theme	sulfate	462:468	arg1	amounts					436:442	Smaller amounts	428:442	Smaller amounts (12 %) of heparan sulfate	428:468	Smaller amounts (12 %) of heparan sulfate was also present which was made of more highly sulfated chains of molecular weight of 21-22 kDa and a less sulfated low molecular weight (< 10 kDa) heterogeneous partially degraded heparan sulfate.
27752801	6	5	theme	sulfate	462:468	arg1	%					448:448	12 %	445:448	12 %	445:448	Smaller amounts (12 %) of heparan sulfate was also present which was made of more highly sulfated chains of molecular weight of 21-22 kDa and a less sulfated low molecular weight (< 10 kDa) heterogeneous partially degraded heparan sulfate.
27752801	7	6	theme	GAGs	764:767	arg1	source					732:737	an inexpensive and readily available source	695:737	an inexpensive and readily available source of muscle tissue-derived GAGs	695:767	Chicken gizzard represents an inexpensive and readily available source of muscle tissue-derived GAGs.
27752801	6	7	theme	heparan	454:460	arg1	sulfate					462:468	heparan sulfate	454:468	heparan sulfate	454:468	Smaller amounts (12 %) of heparan sulfate was also present which was made of more highly sulfated chains of molecular weight of 21-22 kDa and a less sulfated low molecular weight (< 10 kDa) heterogeneous partially degraded heparan sulfate.
27752801	0	8	from	gizzard	52:58	arg1	Glycosaminoglycans					0:17	Glycosaminoglycans	0:17	Glycosaminoglycans	0:17	Glycosaminoglycans from chicken muscular stomach or gizzard.
27752801	3	9	theme	typical	216:222	arg1	value					224:228	a typical value	214:228	% a typical value	212:228	% a typical value observed for a muscle tissue.
27752801	6	10	theme	heterogeneous	618:630	arg1	sulfate					659:665	heterogeneous partially degraded heparan sulfate	618:665	heterogeneous partially degraded heparan sulfate	618:665	Smaller amounts (12 %) of heparan sulfate was also present which was made of more highly sulfated chains of molecular weight of 21-22 kDa and a less sulfated low molecular weight (< 10 kDa) heterogeneous partially degraded heparan sulfate.
27752801	3	11	dep	%	212:212	arg1	value					224:228	a typical value	214:228	% a typical value	212:228	% a typical value observed for a muscle tissue.
27752801	1	12	dep	stomach	119:125	arg1	the					106:108	the	106:108	the	106:108	Glycosaminoglycans (GAGs) were prepared from the muscular stomach or gizzard of the chicken.
27752801	4	13	theme	major	264:268	arg1	chondroitin-6-sulfate					290:310	chondroitin-6-sulfate	290:310	chondroitin-6-sulfate	290:310	The major GAG components were chondroitin-6-sulfate and chondroitin-4-sulfate (~64 %) of molecular weight 21-22 kDa.
27752801	4	13	theme	major	264:268	arg1	components					274:283	The major GAG components	260:283	The major GAG components	260:283	The major GAG components were chondroitin-6-sulfate and chondroitin-4-sulfate (~64 %) of molecular weight 21-22 kDa.
27752801	6	14	theme	molecular	536:544	arg1	weight					546:551	molecular weight	536:551	molecular weight of 21-22 kDa	536:564	Smaller amounts (12 %) of heparan sulfate was also present which was made of more highly sulfated chains of molecular weight of 21-22 kDa and a less sulfated low molecular weight (< 10 kDa) heterogeneous partially degraded heparan sulfate.
27752801	7	15	link	tissue-derived	749:762	arg1	GAGs					764:767	muscle tissue-derived GAGs	742:767	muscle tissue-derived GAGs	742:767	Chicken gizzard represents an inexpensive and readily available source of muscle tissue-derived GAGs.
27752801	7	16	theme	muscle	742:747	arg1	GAGs					764:767	muscle tissue-derived GAGs	742:767	muscle tissue-derived GAGs	742:767	Chicken gizzard represents an inexpensive and readily available source of muscle tissue-derived GAGs.
27752801	4	17	theme	molecular	349:357	arg1	weight					359:364	molecular weight 21-22 kDa	349:374	molecular weight 21-22 kDa	349:374	The major GAG components were chondroitin-6-sulfate and chondroitin-4-sulfate (~64 %) of molecular weight 21-22 kDa.
27752801	6	18	theme	<	608:608	arg1	weight					600:605	a less sulfated low molecular weight	570:605	a less sulfated low molecular weight (< 10 kDa)	570:616	Smaller amounts (12 %) of heparan sulfate was also present which was made of more highly sulfated chains of molecular weight of 21-22 kDa and a less sulfated low molecular weight (< 10 kDa) heterogeneous partially degraded heparan sulfate.
27752801	6	18	theme	<	608:608	arg1	10 kDa					610:615	< 10 kDa	608:615	< 10 kDa	608:615	Smaller amounts (12 %) of heparan sulfate was also present which was made of more highly sulfated chains of molecular weight of 21-22 kDa and a less sulfated low molecular weight (< 10 kDa) heterogeneous partially degraded heparan sulfate.
27752801	0	19	theme	muscular	32:39	arg1	gizzard					52:58	chicken muscular stomach or gizzard	24:58	chicken muscular stomach or gizzard	24:58	Glycosaminoglycans from chicken muscular stomach or gizzard.
27752801	7	20	theme	tissue-derived	749:762	arg1	GAGs					764:767	muscle tissue-derived GAGs	742:767	muscle tissue-derived GAGs	742:767	Chicken gizzard represents an inexpensive and readily available source of muscle tissue-derived GAGs.
27752801	0	21	theme	chicken	24:30	arg1	gizzard					52:58	chicken muscular stomach or gizzard	24:58	chicken muscular stomach or gizzard	24:58	Glycosaminoglycans from chicken muscular stomach or gizzard.
27752801	3	22	theme	muscle	245:250	arg1	tissue					252:257	a muscle tissue	243:257	a muscle tissue	243:257	% a typical value observed for a muscle tissue.
27752801	2	23	theme	GAGs	169:172	arg1	content					158:164	The content	154:164	The content of GAGs on a dry weight basis	154:194	The content of GAGs on a dry weight basis contains 0.4 wt.
27752801	6	24	theme	weight	600:605	arg1	chains					526:531	more highly sulfated chains	505:531	more highly sulfated chains of molecular weight of 21-22 kDa and a less sulfated low molecular weight (< 10 kDa) heterogeneous partially degraded heparan sulfate	505:665	Smaller amounts (12 %) of heparan sulfate was also present which was made of more highly sulfated chains of molecular weight of 21-22 kDa and a less sulfated low molecular weight (< 10 kDa) heterogeneous partially degraded heparan sulfate.
27752801	0	25	theme	or	49:50	arg1	gizzard					52:58	chicken muscular stomach or gizzard	24:58	chicken muscular stomach or gizzard	24:58	Glycosaminoglycans from chicken muscular stomach or gizzard.
27752801	6	26	theme	molecular	590:598	arg1	weight					600:605	a less sulfated low molecular weight	570:605	a less sulfated low molecular weight (< 10 kDa)	570:616	Smaller amounts (12 %) of heparan sulfate was also present which was made of more highly sulfated chains of molecular weight of 21-22 kDa and a less sulfated low molecular weight (< 10 kDa) heterogeneous partially degraded heparan sulfate.
27752801	6	26	theme	molecular	590:598	arg1	10 kDa					610:615	< 10 kDa	608:615	< 10 kDa	608:615	Smaller amounts (12 %) of heparan sulfate was also present which was made of more highly sulfated chains of molecular weight of 21-22 kDa and a less sulfated low molecular weight (< 10 kDa) heterogeneous partially degraded heparan sulfate.
27752801	0	27	theme	stomach	41:47	arg1	gizzard					52:58	chicken muscular stomach or gizzard	24:58	chicken muscular stomach or gizzard	24:58	Glycosaminoglycans from chicken muscular stomach or gizzard.
27752801	7	28	theme	available	722:730	arg1	source					732:737	an inexpensive and readily available source	695:737	an inexpensive and readily available source of muscle tissue-derived GAGs	695:767	Chicken gizzard represents an inexpensive and readily available source of muscle tissue-derived GAGs.
27752801	6	29	dep	weight	546:551	arg1	sulfate					659:665	heterogeneous partially degraded heparan sulfate	618:665	heterogeneous partially degraded heparan sulfate	618:665	Smaller amounts (12 %) of heparan sulfate was also present which was made of more highly sulfated chains of molecular weight of 21-22 kDa and a less sulfated low molecular weight (< 10 kDa) heterogeneous partially degraded heparan sulfate.
27752801	1	30	theme	muscular	110:117	arg1	stomach					119:125	stomach	119:125	stomach	119:125	Glycosaminoglycans (GAGs) were prepared from the muscular stomach or gizzard of the chicken.
27752801	7	31	theme	inexpensive	698:708	arg1	source					732:737	an inexpensive and readily available source	695:737	an inexpensive and readily available source of muscle tissue-derived GAGs	695:767	Chicken gizzard represents an inexpensive and readily available source of muscle tissue-derived GAGs.
27752801	6	32	theme	Smaller	428:434	arg1	sulfate					462:468	heparan sulfate	454:468	heparan sulfate	454:468	Smaller amounts (12 %) of heparan sulfate was also present which was made of more highly sulfated chains of molecular weight of 21-22 kDa and a less sulfated low molecular weight (< 10 kDa) heterogeneous partially degraded heparan sulfate.
27752801	6	32	theme	Smaller	428:434	arg1	amounts					436:442	Smaller amounts	428:442	Smaller amounts (12 %) of heparan sulfate	428:468	Smaller amounts (12 %) of heparan sulfate was also present which was made of more highly sulfated chains of molecular weight of 21-22 kDa and a less sulfated low molecular weight (< 10 kDa) heterogeneous partially degraded heparan sulfate.
27752801	6	32	theme	Smaller	428:434	arg1	%					448:448	12 %	445:448	12 %	445:448	Smaller amounts (12 %) of heparan sulfate was also present which was made of more highly sulfated chains of molecular weight of 21-22 kDa and a less sulfated low molecular weight (< 10 kDa) heterogeneous partially degraded heparan sulfate.
27752801	2	33	theme	weight	183:188	arg1	basis					190:194	a dry weight basis	177:194	a dry weight basis	177:194	The content of GAGs on a dry weight basis contains 0.4 wt.
27752801	5	34	contain	had	396:398	arg1	%					393:393	~24 %	389:393	~24 %	389:393	Hyaluronan (~24 %) had a molecular weight 120 kDa.
27752801	5	34	contain	had	396:398	arg2	120 kDa					419:425	a molecular weight 120 kDa	400:425	a molecular weight 120 kDa	400:425	Hyaluronan (~24 %) had a molecular weight 120 kDa.
27752801	5	34	contain	had	396:398	arg1	Hyaluronan					377:386	Hyaluronan	377:386	Hyaluronan (~24 %)	377:394	Hyaluronan (~24 %) had a molecular weight 120 kDa.
27752801	6	35	theme	low	586:588	arg1	weight					600:605	a less sulfated low molecular weight	570:605	a less sulfated low molecular weight (< 10 kDa)	570:616	Smaller amounts (12 %) of heparan sulfate was also present which was made of more highly sulfated chains of molecular weight of 21-22 kDa and a less sulfated low molecular weight (< 10 kDa) heterogeneous partially degraded heparan sulfate.
27752801	6	35	theme	low	586:588	arg1	10 kDa					610:615	< 10 kDa	608:615	< 10 kDa	608:615	Smaller amounts (12 %) of heparan sulfate was also present which was made of more highly sulfated chains of molecular weight of 21-22 kDa and a less sulfated low molecular weight (< 10 kDa) heterogeneous partially degraded heparan sulfate.
27752801	2	36	theme	dry	179:181	arg1	basis					190:194	a dry weight basis	177:194	a dry weight basis	177:194	The content of GAGs on a dry weight basis contains 0.4 wt.
27752801	6	37	theme	sulfated	577:584	arg1	weight					600:605	a less sulfated low molecular weight	570:605	a less sulfated low molecular weight (< 10 kDa)	570:616	Smaller amounts (12 %) of heparan sulfate was also present which was made of more highly sulfated chains of molecular weight of 21-22 kDa and a less sulfated low molecular weight (< 10 kDa) heterogeneous partially degraded heparan sulfate.
27752801	6	37	theme	sulfated	577:584	arg1	10 kDa					610:615	< 10 kDa	608:615	< 10 kDa	608:615	Smaller amounts (12 %) of heparan sulfate was also present which was made of more highly sulfated chains of molecular weight of 21-22 kDa and a less sulfated low molecular weight (< 10 kDa) heterogeneous partially degraded heparan sulfate.
27752801	5	38	theme	molecular	402:410	arg1	120 kDa					419:425	a molecular weight 120 kDa	400:425	a molecular weight 120 kDa	400:425	Hyaluronan (~24 %) had a molecular weight 120 kDa.
27752801	2	39	contain	contains	196:203	arg1	content					158:164	The content	154:164	The content of GAGs on a dry weight basis	154:194	The content of GAGs on a dry weight basis contains 0.4 wt.
27752801	2	39	contain	contains	196:203	arg2	0.4 wt					205:210	0.4 wt	205:210	0.4 wt	205:210	The content of GAGs on a dry weight basis contains 0.4 wt.
27752801	2	40	from	content	158:164	arg1	basis					190:194	a dry weight basis	177:194	a dry weight basis	177:194	The content of GAGs on a dry weight basis contains 0.4 wt.
27752801	4	41	theme	GAG	270:272	arg1	chondroitin-6-sulfate					290:310	chondroitin-6-sulfate	290:310	chondroitin-6-sulfate	290:310	The major GAG components were chondroitin-6-sulfate and chondroitin-4-sulfate (~64 %) of molecular weight 21-22 kDa.
27752801	4	41	theme	GAG	270:272	arg1	components					274:283	The major GAG components	260:283	The major GAG components	260:283	The major GAG components were chondroitin-6-sulfate and chondroitin-4-sulfate (~64 %) of molecular weight 21-22 kDa.
27752801	5	42	theme	weight	412:417	arg1	120 kDa					419:425	a molecular weight 120 kDa	400:425	a molecular weight 120 kDa	400:425	Hyaluronan (~24 %) had a molecular weight 120 kDa.
27752801	7	43	theme	Chicken	668:674	arg1	gizzard					676:682	Chicken gizzard	668:682	Chicken gizzard	668:682	Chicken gizzard represents an inexpensive and readily available source of muscle tissue-derived GAGs.
27752801	6	44	theme	sulfated	517:524	arg1	chains					526:531	more highly sulfated chains	505:531	more highly sulfated chains of molecular weight of 21-22 kDa and a less sulfated low molecular weight (< 10 kDa) heterogeneous partially degraded heparan sulfate	505:665	Smaller amounts (12 %) of heparan sulfate was also present which was made of more highly sulfated chains of molecular weight of 21-22 kDa and a less sulfated low molecular weight (< 10 kDa) heterogeneous partially degraded heparan sulfate.
27752801	6	45	theme	heparan	651:657	arg1	sulfate					659:665	heterogeneous partially degraded heparan sulfate	618:665	heterogeneous partially degraded heparan sulfate	618:665	Smaller amounts (12 %) of heparan sulfate was also present which was made of more highly sulfated chains of molecular weight of 21-22 kDa and a less sulfated low molecular weight (< 10 kDa) heterogeneous partially degraded heparan sulfate.
29027584	9	0	theme	third	1303:1307	arg1	wall					1309:1312	The third wall	1299:1312	The third wall (W3)	1299:1317	The third wall (W3) is thick and composed entirely of callose, and the fourth wall (W4) is built of cellulose heavily crosslinked by galactoxyloglucan hemicelluloses.
29027584	9	0	theme	third	1303:1307	arg1	W3					1315:1316	W3	1315:1316	W3	1315:1316	The third wall (W3) is thick and composed entirely of callose, and the fourth wall (W4) is built of cellulose heavily crosslinked by galactoxyloglucan hemicelluloses.
29027584	0	1	contain	contain	55:61	arg1	walls					49:53	The Ceratopteris (fern) developing motile gamete walls	0:53	The Ceratopteris (fern) developing motile gamete walls	0:53	The Ceratopteris (fern) developing motile gamete walls contain diverse polysaccharides, but not pectin.
29027584	0	1	contain	contain	55:61	arg2	pectin					96:101	pectin	96:101	pectin	96:101	The Ceratopteris (fern) developing motile gamete walls contain diverse polysaccharides, but not pectin.
29027584	0	1	contain	contain	55:61	arg2	diverse polysaccharides					63:85	diverse polysaccharides	63:85	diverse polysaccharides	63:85	The Ceratopteris (fern) developing motile gamete walls contain diverse polysaccharides, but not pectin.
29027584	1	2	theme	sparse	233:238	arg1	cellulose					240:248	sparse cellulose	233:248	sparse cellulose	233:248	Unlike most plant cell walls, the five consecutive walls laid down during spermatogenesis in the model fern Ceratopteris contain sparse cellulose, lack pectin and are enriched with callose and hemicelluloses.
29027584	5	3	theme	xylan-containing	920:935	arg1	hemicelluloses					937:950	xylan-containing hemicelluloses	920:950	xylan-containing hemicelluloses	920:950	Using histochemistry and immunogold localizations, we examined the distribution of callose, cellulose, mannan and xylan-containing hemicelluloses, and homogalacturonan (HG) pectins in the special walls deposited during spermatogenesis in Ceratopteris.
29027584	6	4	contain	has	1103:1105	arg1	each					1098:1101	each	1098:1101	each	1098:1101	Five walls are produced in sequence and each has a unique fate.
29027584	6	4	contain	has	1103:1105	arg2	fate					1116:1119	a unique fate	1107:1119	a unique fate	1107:1119	Five walls are produced in sequence and each has a unique fate.
29027584	7	5	contain	contains	1142:1149	arg1	wall					1132:1135	The first wall	1122:1135	The first wall (W1)	1122:1140	The first wall (W1) contains callose and sparse xylan-containing hemicelluloses.
29027584	7	5	contain	contains	1142:1149	arg2	hemicelluloses					1187:1200	callose and sparse xylan-containing hemicelluloses	1151:1200	callose and sparse xylan-containing hemicelluloses	1151:1200	The first wall (W1) contains callose and sparse xylan-containing hemicelluloses.
29027584	7	5	contain	contains	1142:1149	arg1	W1					1138:1139	W1	1138:1139	W1	1138:1139	The first wall (W1) contains callose and sparse xylan-containing hemicelluloses.
29027584	4	6	theme	formation	688:696	arg1	composition					706:716	the composition	702:716	the composition of these walls during sperm cell differentiation in plants that produce swimming sperm	702:803	Other than the detection of callose and general wall polysaccharides in scattered groups, little is known about the sequence of wall formation and the composition of these walls during sperm cell differentiation in plants that produce swimming sperm.
29027584	4	6	theme	formation	688:696	arg1	sequence					671:678	the sequence	667:678	the sequence of wall formation	667:696	Other than the detection of callose and general wall polysaccharides in scattered groups, little is known about the sequence of wall formation and the composition of these walls during sperm cell differentiation in plants that produce swimming sperm.
29027584	5	7	theme	special	994:1000	arg1	walls					1002:1006	the special walls	990:1006	the special walls deposited during spermatogenesis in Ceratopteris	990:1055	Using histochemistry and immunogold localizations, we examined the distribution of callose, cellulose, mannan and xylan-containing hemicelluloses, and homogalacturonan (HG) pectins in the special walls deposited during spermatogenesis in Ceratopteris.
29027584	7	8	theme	first	1126:1130	arg1	wall					1132:1135	The first wall	1122:1135	The first wall (W1)	1122:1140	The first wall (W1) contains callose and sparse xylan-containing hemicelluloses.
29027584	7	8	theme	first	1126:1130	arg1	W1					1138:1139	W1	1138:1139	W1	1138:1139	The first wall (W1) contains callose and sparse xylan-containing hemicelluloses.
29027584	3	9	theme	gametes	546:552	arg1	maturation					521:530	maturation	521:530	maturation	521:530	During spermatogenesis, unique walls are formed that are essential to the appropriate development and maturation of the motile gametes.
29027584	3	9	theme	gametes	546:552	arg1	development					505:515	appropriate development	493:515	appropriate development	493:515	During spermatogenesis, unique walls are formed that are essential to the appropriate development and maturation of the motile gametes.
29027584	6	10	dep	has	1103:1105	arg1	sequence and					1085:1096	sequence and	1085:1096	sequence and	1085:1096	Five walls are produced in sequence and each has a unique fate.
29027584	4	11	theme	general	595:601	arg1	polysaccharides					608:622	callose and general wall polysaccharides	583:622	callose and general wall polysaccharides	583:622	Other than the detection of callose and general wall polysaccharides in scattered groups, little is known about the sequence of wall formation and the composition of these walls during sperm cell differentiation in plants that produce swimming sperm.
29027584	11	12	theme	HG	1637:1638	arg1	pectins					1640:1646	unesterified HG pectins	1624:1646	unesterified HG pectins	1624:1646	We detected no esterified or unesterified HG pectins in any of the walls laid down during spermatogenesis.
29027584	7	13	theme	callose	1151:1157	arg1	hemicelluloses					1187:1200	callose and sparse xylan-containing hemicelluloses	1151:1200	callose and sparse xylan-containing hemicelluloses	1151:1200	The first wall (W1) contains callose and sparse xylan-containing hemicelluloses.
29027584	12	14	from	modifications	1727:1739	arg1	walls					1749:1753	cell walls	1744:1753	cell walls associated with motile gametes	1744:1784	To consider evolutionary modifications in cell walls associated with motile gametes, comparisons are presented with male gametophyte and spermatogenous cell walls across plant groups.
29027584	10	15	dep	changes	1549:1555	arg1	shape					1557:1561	shape	1557:1561	shape	1557:1561	Wall five (W5) is an arabinogalactan protein (AGP)-rich matrix in which the gamete changes shape and multiple flagella elongate.
29027584	10	15	dep	changes	1549:1555	arg1	flagella					1576:1583	multiple flagella	1567:1583	multiple flagella	1567:1583	Wall five (W5) is an arabinogalactan protein (AGP)-rich matrix in which the gamete changes shape and multiple flagella elongate.
29027584	4	16	from	detection	570:578	arg1	groups					637:642	scattered groups	627:642	scattered groups	627:642	Other than the detection of callose and general wall polysaccharides in scattered groups, little is known about the sequence of wall formation and the composition of these walls during sperm cell differentiation in plants that produce swimming sperm.
29027584	5	17	from	spermatogenesis	1025:1039	arg1	Ceratopteris					1044:1055	Ceratopteris	1044:1055	Ceratopteris	1044:1055	Using histochemistry and immunogold localizations, we examined the distribution of callose, cellulose, mannan and xylan-containing hemicelluloses, and homogalacturonan (HG) pectins in the special walls deposited during spermatogenesis in Ceratopteris.
29027584	1	18	theme	consecutive	143:153	arg1	enriched					271:278	enriched	271:278	enriched	271:278	Unlike most plant cell walls, the five consecutive walls laid down during spermatogenesis in the model fern Ceratopteris contain sparse cellulose, lack pectin and are enriched with callose and hemicelluloses.
29027584	1	18	theme	consecutive	143:153	arg1	walls					155:159	the five consecutive walls	134:159	the five consecutive walls laid down during spermatogenesis in the model fern Ceratopteris	134:223	Unlike most plant cell walls, the five consecutive walls laid down during spermatogenesis in the model fern Ceratopteris contain sparse cellulose, lack pectin and are enriched with callose and hemicelluloses.
29027584	10	19	theme	multiple	1567:1574	arg1	flagella					1576:1583	multiple flagella	1567:1583	multiple flagella	1567:1583	Wall five (W5) is an arabinogalactan protein (AGP)-rich matrix in which the gamete changes shape and multiple flagella elongate.
29027584	4	20	theme	callose	583:589	arg1	polysaccharides					608:622	callose and general wall polysaccharides	583:622	callose and general wall polysaccharides	583:622	Other than the detection of callose and general wall polysaccharides in scattered groups, little is known about the sequence of wall formation and the composition of these walls during sperm cell differentiation in plants that produce swimming sperm.
29027584	1	21	theme	fern	207:210	arg1	Ceratopteris					212:223	the model fern Ceratopteris	197:223	the model fern Ceratopteris	197:223	Unlike most plant cell walls, the five consecutive walls laid down during spermatogenesis in the model fern Ceratopteris contain sparse cellulose, lack pectin and are enriched with callose and hemicelluloses.
29027584	6	22	theme	unique	1109:1114	arg1	fate					1116:1119	a unique fate	1107:1119	a unique fate	1107:1119	Five walls are produced in sequence and each has a unique fate.
29027584	0	23	theme	Ceratopteris	4:15	arg1	walls					49:53	The Ceratopteris (fern) developing motile gamete walls	0:53	The Ceratopteris (fern) developing motile gamete walls	0:53	The Ceratopteris (fern) developing motile gamete walls contain diverse polysaccharides, but not pectin.
29027584	11	24	theme	unesterified	1624:1635	arg1	pectins					1640:1646	unesterified HG pectins	1624:1646	unesterified HG pectins	1624:1646	We detected no esterified or unesterified HG pectins in any of the walls laid down during spermatogenesis.
29027584	1	25	with	enriched	271:278	arg1	callose					285:291	callose	285:291	callose	285:291	Unlike most plant cell walls, the five consecutive walls laid down during spermatogenesis in the model fern Ceratopteris contain sparse cellulose, lack pectin and are enriched with callose and hemicelluloses.
29027584	1	25	with	enriched	271:278	arg1	hemicelluloses					297:310	hemicelluloses	297:310	hemicelluloses	297:310	Unlike most plant cell walls, the five consecutive walls laid down during spermatogenesis in the model fern Ceratopteris contain sparse cellulose, lack pectin and are enriched with callose and hemicelluloses.
29027584	3	26	dep	development	505:515	arg1	the					489:491	the	489:491	the	489:491	During spermatogenesis, unique walls are formed that are essential to the appropriate development and maturation of the motile gametes.
29027584	2	27	theme	male	381:384	arg1	gametes					386:392	swimming male gametes	372:392	swimming male gametes for sexual reproduction	372:416	Seed-free plants like bryophytes and pteridophytes produce swimming male gametes for sexual reproduction.
29027584	12	28	theme	plant	1872:1876	arg1	groups					1878:1883	plant groups	1872:1883	plant groups	1872:1883	To consider evolutionary modifications in cell walls associated with motile gametes, comparisons are presented with male gametophyte and spermatogenous cell walls across plant groups.
29027584	4	29	theme	walls	727:731	arg1	composition					706:716	the composition	702:716	the composition of these walls during sperm cell differentiation in plants that produce swimming sperm	702:803	Other than the detection of callose and general wall polysaccharides in scattered groups, little is known about the sequence of wall formation and the composition of these walls during sperm cell differentiation in plants that produce swimming sperm.
29027584	4	29	theme	walls	727:731	arg1	sequence					671:678	the sequence	667:678	the sequence of wall formation	667:696	Other than the detection of callose and general wall polysaccharides in scattered groups, little is known about the sequence of wall formation and the composition of these walls during sperm cell differentiation in plants that produce swimming sperm.
29027584	2	30	theme	swimming	372:379	arg1	gametes					386:392	swimming male gametes	372:392	swimming male gametes for sexual reproduction	372:416	Seed-free plants like bryophytes and pteridophytes produce swimming male gametes for sexual reproduction.
29027584	0	31	theme	fern	18:21	arg1	walls					49:53	The Ceratopteris (fern) developing motile gamete walls	0:53	The Ceratopteris (fern) developing motile gamete walls	0:53	The Ceratopteris (fern) developing motile gamete walls contain diverse polysaccharides, but not pectin.
29027584	12	32	theme	evolutionary	1714:1725	arg1	modifications					1727:1739	evolutionary modifications	1714:1739	evolutionary modifications in cell walls associated with motile gametes	1714:1784	To consider evolutionary modifications in cell walls associated with motile gametes, comparisons are presented with male gametophyte and spermatogenous cell walls across plant groups.
29027584	12	33	theme	cell	1854:1857	arg1	walls					1859:1863	spermatogenous cell walls	1839:1863	spermatogenous cell walls across plant groups	1839:1883	To consider evolutionary modifications in cell walls associated with motile gametes, comparisons are presented with male gametophyte and spermatogenous cell walls across plant groups.
29027584	4	34	from	differentiation	751:765	arg1	plants					770:775	plants	770:775	plants that produce swimming sperm	770:803	Other than the detection of callose and general wall polysaccharides in scattered groups, little is known about the sequence of wall formation and the composition of these walls during sperm cell differentiation in plants that produce swimming sperm.
29027584	4	35	theme	cell	746:749	arg1	differentiation					751:765	sperm cell differentiation	740:765	sperm cell differentiation in plants that produce swimming sperm	740:803	Other than the detection of callose and general wall polysaccharides in scattered groups, little is known about the sequence of wall formation and the composition of these walls during sperm cell differentiation in plants that produce swimming sperm.
29027584	8	36	theme	xylan-containing	1266:1281	arg1	hemicelluloses					1283:1296	xylan-containing hemicelluloses	1266:1296	xylan-containing hemicelluloses	1266:1296	Wall two (W2) is thin and composed of cellulose crosslinked by xylan-containing hemicelluloses.
29027584	5	37	theme	immunogold	831:840	arg1	localizations					842:854	immunogold localizations	831:854	immunogold localizations	831:854	Using histochemistry and immunogold localizations, we examined the distribution of callose, cellulose, mannan and xylan-containing hemicelluloses, and homogalacturonan (HG) pectins in the special walls deposited during spermatogenesis in Ceratopteris.
29027584	12	38	theme	motile	1771:1776	arg1	gametes					1778:1784	motile gametes	1771:1784	motile gametes	1771:1784	To consider evolutionary modifications in cell walls associated with motile gametes, comparisons are presented with male gametophyte and spermatogenous cell walls across plant groups.
29027584	12	39	theme	spermatogenous	1839:1852	arg1	walls					1859:1863	spermatogenous cell walls	1839:1863	spermatogenous cell walls across plant groups	1839:1883	To consider evolutionary modifications in cell walls associated with motile gametes, comparisons are presented with male gametophyte and spermatogenous cell walls across plant groups.
29027584	4	40	theme	sperm	740:744	arg1	differentiation					751:765	sperm cell differentiation	740:765	sperm cell differentiation in plants that produce swimming sperm	740:803	Other than the detection of callose and general wall polysaccharides in scattered groups, little is known about the sequence of wall formation and the composition of these walls during sperm cell differentiation in plants that produce swimming sperm.
29027584	0	41	theme	developing	24:33	arg1	walls					49:53	The Ceratopteris (fern) developing motile gamete walls	0:53	The Ceratopteris (fern) developing motile gamete walls	0:53	The Ceratopteris (fern) developing motile gamete walls contain diverse polysaccharides, but not pectin.
29027584	3	42	theme	appropriate	493:503	arg1	development					505:515	appropriate development	493:515	appropriate development	493:515	During spermatogenesis, unique walls are formed that are essential to the appropriate development and maturation of the motile gametes.
29027584	10	43	theme	gamete	1542:1547	arg1	changes					1549:1555	the gamete changes shape and multiple flagella	1538:1583	the gamete changes shape and multiple flagella	1538:1583	Wall five (W5) is an arabinogalactan protein (AGP)-rich matrix in which the gamete changes shape and multiple flagella elongate.
29027584	1	44	contain	contain	225:231	arg2	cellulose					240:248	sparse cellulose	233:248	sparse cellulose	233:248	Unlike most plant cell walls, the five consecutive walls laid down during spermatogenesis in the model fern Ceratopteris contain sparse cellulose, lack pectin and are enriched with callose and hemicelluloses.
29027584	1	44	contain	contain	225:231	arg1	enriched					271:278	enriched	271:278	enriched	271:278	Unlike most plant cell walls, the five consecutive walls laid down during spermatogenesis in the model fern Ceratopteris contain sparse cellulose, lack pectin and are enriched with callose and hemicelluloses.
29027584	1	44	contain	contain	225:231	arg1	walls					155:159	the five consecutive walls	134:159	the five consecutive walls laid down during spermatogenesis in the model fern Ceratopteris	134:223	Unlike most plant cell walls, the five consecutive walls laid down during spermatogenesis in the model fern Ceratopteris contain sparse cellulose, lack pectin and are enriched with callose and hemicelluloses.
29027584	5	45	theme	callose	889:895	arg1	distribution					873:884	the distribution	869:884	the distribution of callose	869:895	Using histochemistry and immunogold localizations, we examined the distribution of callose, cellulose, mannan and xylan-containing hemicelluloses, and homogalacturonan (HG) pectins in the special walls deposited during spermatogenesis in Ceratopteris.
29027584	3	46	theme	unique	443:448	arg1	walls					450:454	unique walls	443:454	unique walls	443:454	During spermatogenesis, unique walls are formed that are essential to the appropriate development and maturation of the motile gametes.
29027584	7	47	theme	sparse	1163:1168	arg1	hemicelluloses					1187:1200	callose and sparse xylan-containing hemicelluloses	1151:1200	callose and sparse xylan-containing hemicelluloses	1151:1200	The first wall (W1) contains callose and sparse xylan-containing hemicelluloses.
29027584	12	48	theme	cell	1744:1747	arg1	walls					1749:1753	cell walls	1744:1753	cell walls associated with motile gametes	1744:1784	To consider evolutionary modifications in cell walls associated with motile gametes, comparisons are presented with male gametophyte and spermatogenous cell walls across plant groups.
29027584	2	49	theme	sexual	398:403	arg1	reproduction					405:416	sexual reproduction	398:416	sexual reproduction	398:416	Seed-free plants like bryophytes and pteridophytes produce swimming male gametes for sexual reproduction.
29027584	0	50	theme	gamete	42:47	arg1	walls					49:53	The Ceratopteris (fern) developing motile gamete walls	0:53	The Ceratopteris (fern) developing motile gamete walls	0:53	The Ceratopteris (fern) developing motile gamete walls contain diverse polysaccharides, but not pectin.
29027584	12	51	theme	male	1818:1821	arg1	gametophyte					1823:1833	male gametophyte	1818:1833	male gametophyte	1818:1833	To consider evolutionary modifications in cell walls associated with motile gametes, comparisons are presented with male gametophyte and spermatogenous cell walls across plant groups.
29027584	9	52	theme	cellulose	1399:1407	arg1	built					1390:1394	built	1390:1394	built of cellulose heavily crosslinked by galactoxyloglucan hemicelluloses	1390:1463	The third wall (W3) is thick and composed entirely of callose, and the fourth wall (W4) is built of cellulose heavily crosslinked by galactoxyloglucan hemicelluloses.
29027584	9	52	theme	cellulose	1399:1407	arg1	wall					1377:1380	the fourth wall	1366:1380	the fourth wall (W4)	1366:1385	The third wall (W3) is thick and composed entirely of callose, and the fourth wall (W4) is built of cellulose heavily crosslinked by galactoxyloglucan hemicelluloses.
29027584	0	53	theme	motile	35:40	arg1	walls					49:53	The Ceratopteris (fern) developing motile gamete walls	0:53	The Ceratopteris (fern) developing motile gamete walls	0:53	The Ceratopteris (fern) developing motile gamete walls contain diverse polysaccharides, but not pectin.
29027584	1	54	dep	walls	155:159	arg1	laid					161:164	laid	161:164	laid down during spermatogenesis in the model fern Ceratopteris	161:223	Unlike most plant cell walls, the five consecutive walls laid down during spermatogenesis in the model fern Ceratopteris contain sparse cellulose, lack pectin and are enriched with callose and hemicelluloses.
29027584	1	55	theme	most	111:114	arg1	walls					127:131	most plant cell walls	111:131	most plant cell walls	111:131	Unlike most plant cell walls, the five consecutive walls laid down during spermatogenesis in the model fern Ceratopteris contain sparse cellulose, lack pectin and are enriched with callose and hemicelluloses.
29027584	4	56	theme	swimming	790:797	arg1	sperm					799:803	swimming sperm	790:803	swimming sperm	790:803	Other than the detection of callose and general wall polysaccharides in scattered groups, little is known about the sequence of wall formation and the composition of these walls during sperm cell differentiation in plants that produce swimming sperm.
29027584	1	57	from	spermatogenesis	178:192	arg1	Ceratopteris					212:223	the model fern Ceratopteris	197:223	the model fern Ceratopteris	197:223	Unlike most plant cell walls, the five consecutive walls laid down during spermatogenesis in the model fern Ceratopteris contain sparse cellulose, lack pectin and are enriched with callose and hemicelluloses.
29027584	1	58	theme	plant	116:120	arg1	walls					127:131	most plant cell walls	111:131	most plant cell walls	111:131	Unlike most plant cell walls, the five consecutive walls laid down during spermatogenesis in the model fern Ceratopteris contain sparse cellulose, lack pectin and are enriched with callose and hemicelluloses.
29027584	9	59	theme	fourth	1370:1375	arg1	W4					1383:1384	W4	1383:1384	W4	1383:1384	The third wall (W3) is thick and composed entirely of callose, and the fourth wall (W4) is built of cellulose heavily crosslinked by galactoxyloglucan hemicelluloses.
29027584	9	59	theme	fourth	1370:1375	arg1	built					1390:1394	built	1390:1394	built of cellulose heavily crosslinked by galactoxyloglucan hemicelluloses	1390:1463	The third wall (W3) is thick and composed entirely of callose, and the fourth wall (W4) is built of cellulose heavily crosslinked by galactoxyloglucan hemicelluloses.
29027584	9	59	theme	fourth	1370:1375	arg1	wall					1377:1380	the fourth wall	1366:1380	the fourth wall (W4)	1366:1385	The third wall (W3) is thick and composed entirely of callose, and the fourth wall (W4) is built of cellulose heavily crosslinked by galactoxyloglucan hemicelluloses.
29027584	1	60	theme	model	201:205	arg1	Ceratopteris					212:223	the model fern Ceratopteris	197:223	the model fern Ceratopteris	197:223	Unlike most plant cell walls, the five consecutive walls laid down during spermatogenesis in the model fern Ceratopteris contain sparse cellulose, lack pectin and are enriched with callose and hemicelluloses.
29027584	3	61	theme	motile	539:544	arg1	gametes					546:552	the motile gametes	535:552	the motile gametes	535:552	During spermatogenesis, unique walls are formed that are essential to the appropriate development and maturation of the motile gametes.
29027584	1	62	theme	cell	122:125	arg1	walls					127:131	most plant cell walls	111:131	most plant cell walls	111:131	Unlike most plant cell walls, the five consecutive walls laid down during spermatogenesis in the model fern Ceratopteris contain sparse cellulose, lack pectin and are enriched with callose and hemicelluloses.
29027584	2	63	theme	Seed-free	313:321	arg1	plants					323:328	Seed-free plants	313:328	Seed-free plants like bryophytes and pteridophytes	313:362	Seed-free plants like bryophytes and pteridophytes produce swimming male gametes for sexual reproduction.
29027584	10	64	theme	-rich	1516:1520	arg1	matrix					1522:1527	an arabinogalactan protein (AGP)-rich matrix	1484:1527	an arabinogalactan protein (AGP)-rich matrix in which the gamete changes shape and multiple flagella elongate	1484:1592	Wall five (W5) is an arabinogalactan protein (AGP)-rich matrix in which the gamete changes shape and multiple flagella elongate.
29027584	10	64	theme	-rich	1516:1520	arg1	Wall					1466:1469	Wall five	1466:1474	Wall five (W5)	1466:1479	Wall five (W5) is an arabinogalactan protein (AGP)-rich matrix in which the gamete changes shape and multiple flagella elongate.
29027584	9	65	theme	galactoxyloglucan	1432:1448	arg1	hemicelluloses					1450:1463	galactoxyloglucan hemicelluloses	1432:1463	galactoxyloglucan hemicelluloses	1432:1463	The third wall (W3) is thick and composed entirely of callose, and the fourth wall (W4) is built of cellulose heavily crosslinked by galactoxyloglucan hemicelluloses.
29027584	4	66	theme	wall	683:686	arg1	formation					688:696	wall formation	683:696	wall formation	683:696	Other than the detection of callose and general wall polysaccharides in scattered groups, little is known about the sequence of wall formation and the composition of these walls during sperm cell differentiation in plants that produce swimming sperm.
29027584	4	67	theme	scattered	627:635	arg1	groups					637:642	scattered groups	627:642	scattered groups	627:642	Other than the detection of callose and general wall polysaccharides in scattered groups, little is known about the sequence of wall formation and the composition of these walls during sperm cell differentiation in plants that produce swimming sperm.
29027584	7	68	theme	xylan-containing	1170:1185	arg1	hemicelluloses					1187:1200	callose and sparse xylan-containing hemicelluloses	1151:1200	callose and sparse xylan-containing hemicelluloses	1151:1200	The first wall (W1) contains callose and sparse xylan-containing hemicelluloses.
29027584	4	69	theme	wall	603:606	arg1	polysaccharides					608:622	callose and general wall polysaccharides	583:622	callose and general wall polysaccharides	583:622	Other than the detection of callose and general wall polysaccharides in scattered groups, little is known about the sequence of wall formation and the composition of these walls during sperm cell differentiation in plants that produce swimming sperm.
29027584	4	70	theme	polysaccharides	608:622	arg1	detection					570:578	the detection	566:578	the detection of callose and general wall polysaccharides in scattered groups	566:642	Other than the detection of callose and general wall polysaccharides in scattered groups, little is known about the sequence of wall formation and the composition of these walls during sperm cell differentiation in plants that produce swimming sperm.
29027584	11	71	dep	the walls	1658:1666	arg1	laid					1668:1671	laid	1668:1671	laid down during spermatogenesis	1668:1699	We detected no esterified or unesterified HG pectins in any of the walls laid down during spermatogenesis.
27716345	3	0	theme	high	465:468	arg1	sources					558:564	(main) carbon sources	544:564	(main) carbon sources	544:564	Throughout cultivation of two K. baliensis strains (DSM 14400, NBRC 16680) on sucrose-deficient media, we found that both strains still produce high amounts of mucous, water-soluble substances from mannitol and glycerol as (main) carbon sources.
27716345	3	0	theme	high	465:468	arg1	substances					503:512	mucous, water-soluble substances	481:512	mucous, water-soluble substances from mannitol and glycerol	481:539	Throughout cultivation of two K. baliensis strains (DSM 14400, NBRC 16680) on sucrose-deficient media, we found that both strains still produce high amounts of mucous, water-soluble substances from mannitol and glycerol as (main) carbon sources.
27716345	3	0	theme	high	465:468	arg1	amounts					470:476	high amounts	465:476	high amounts of mucous, water-soluble substances from mannitol and glycerol	465:539	Throughout cultivation of two K. baliensis strains (DSM 14400, NBRC 16680) on sucrose-deficient media, we found that both strains still produce high amounts of mucous, water-soluble substances from mannitol and glycerol as (main) carbon sources.
27716345	8	1	theme	baliensis	1108:1116	arg1	strains					1118:1124	both K. baliensis strains	1100:1124	both K. baliensis strains	1100:1124	Additionally, both K. baliensis strains harbor identical gum-like clusters, which are related to the well characterized gum cluster coding for xanthan synthesis in Xanthomanas campestris and show highest similarity with gum-like heteropolysaccharide (HePS) clusters from other acetic acid bacteria such as Gluconacetobacter diazotrophicus and Komagataeibacter xylinus.
27716345	11	2	theme	genome	2058:2063	arg1	analysis					2065:2072	comparative sugar monomer and genome analysis	2028:2072	analysis	2065:2072	CONCLUSIONS By comparative sugar monomer and genome analysis, the polymeric substances secreted by K. baliensis can be considered as unique HePS.
27716345	7	3	theme	baliensis	1064:1072	arg1	NBRC					1074:1077	K. baliensis NBRC 16680	1061:1083	K. baliensis NBRC 16680	1061:1083	In K. baliensis DSM 14400 plasmid encoded cellulose synthase genes and fragments of truncated levansucrase operons could be assigned in contrast to K. baliensis NBRC 16680.
27716345	3	4	from	amounts	470:476	arg1	mannitol					519:526	mannitol	519:526	mannitol	519:526	Throughout cultivation of two K. baliensis strains (DSM 14400, NBRC 16680) on sucrose-deficient media, we found that both strains still produce high amounts of mucous, water-soluble substances from mannitol and glycerol as (main) carbon sources.
27716345	3	4	from	amounts	470:476	arg1	glycerol					532:539	glycerol	532:539	glycerol	532:539	Throughout cultivation of two K. baliensis strains (DSM 14400, NBRC 16680) on sucrose-deficient media, we found that both strains still produce high amounts of mucous, water-soluble substances from mannitol and glycerol as (main) carbon sources.
27716345	3	5	theme	substances	503:512	arg1	sources					558:564	(main) carbon sources	544:564	(main) carbon sources	544:564	Throughout cultivation of two K. baliensis strains (DSM 14400, NBRC 16680) on sucrose-deficient media, we found that both strains still produce high amounts of mucous, water-soluble substances from mannitol and glycerol as (main) carbon sources.
27716345	3	5	theme	substances	503:512	arg1	substances					503:512	mucous, water-soluble substances	481:512	mucous, water-soluble substances from mannitol and glycerol	481:539	Throughout cultivation of two K. baliensis strains (DSM 14400, NBRC 16680) on sucrose-deficient media, we found that both strains still produce high amounts of mucous, water-soluble substances from mannitol and glycerol as (main) carbon sources.
27716345	3	5	theme	substances	503:512	arg1	amounts					470:476	high amounts	465:476	high amounts of mucous, water-soluble substances from mannitol and glycerol	465:539	Throughout cultivation of two K. baliensis strains (DSM 14400, NBRC 16680) on sucrose-deficient media, we found that both strains still produce high amounts of mucous, water-soluble substances from mannitol and glycerol as (main) carbon sources.
27716345	8	6	from	bacteria	1375:1382	arg1	clusters					1343:1350	gum-like heteropolysaccharide (HePS) clusters	1306:1350	gum-like heteropolysaccharide (HePS) clusters from other acetic acid bacteria such as Gluconacetobacter diazotrophicus and Komagataeibacter xylinus	1306:1452	Additionally, both K. baliensis strains harbor identical gum-like clusters, which are related to the well characterized gum cluster coding for xanthan synthesis in Xanthomanas campestris and show highest similarity with gum-like heteropolysaccharide (HePS) clusters from other acetic acid bacteria such as Gluconacetobacter diazotrophicus and Komagataeibacter xylinus.
27716345	9	7	theme	wildtype	1650:1657	arg1	strain					1659:1664	the wildtype strain	1646:1664	the wildtype strain	1646:1664	A mutant strain of K. baliensis NBRC 16680 lacking EPS production on sucrose-deficient media exhibited a transposon insertion in front of the gumD gene of its gum-like cluster in contrast to the wildtype strain, which indicated the essential role of gumD and of the associated gum genes for production of these new EPS.
27716345	2	8	from	sucrose	312:318	arg1	levan					301:305	ultra-high molecular weight levan	273:305	ultra-high molecular weight levan from sucrose	273:318	Kozakia (K.) baliensis is a relatively new member of AAB, which produces ultra-high molecular weight levan from sucrose.
27716345	8	9	theme	gum-like	1143:1150	arg1	clusters					1152:1159	identical gum-like clusters	1133:1159	identical gum-like clusters	1133:1159	Additionally, both K. baliensis strains harbor identical gum-like clusters, which are related to the well characterized gum cluster coding for xanthan synthesis in Xanthomanas campestris and show highest similarity with gum-like heteropolysaccharide (HePS) clusters from other acetic acid bacteria such as Gluconacetobacter diazotrophicus and Komagataeibacter xylinus.
27716345	11	10	theme	polymeric	2079:2087	arg1	substances					2089:2098	the polymeric substances	2075:2098	the polymeric substances secreted by K. baliensis	2075:2123	CONCLUSIONS By comparative sugar monomer and genome analysis, the polymeric substances secreted by K. baliensis can be considered as unique HePS.
27716345	11	10	theme	polymeric	2079:2087	arg1	HePS					2153:2156	unique HePS	2146:2156	unique HePS	2146:2156	CONCLUSIONS By comparative sugar monomer and genome analysis, the polymeric substances secreted by K. baliensis can be considered as unique HePS.
27716345	8	11	theme	gum	1206:1208	arg1	cluster					1210:1216	the well characterized gum cluster	1183:1216	the well characterized gum cluster coding for xanthan synthesis in Xanthomanas campestris	1183:1271	Additionally, both K. baliensis strains harbor identical gum-like clusters, which are related to the well characterized gum cluster coding for xanthan synthesis in Xanthomanas campestris and show highest similarity with gum-like heteropolysaccharide (HePS) clusters from other acetic acid bacteria such as Gluconacetobacter diazotrophicus and Komagataeibacter xylinus.
27716345	10	12	theme	sugar	1911:1915	arg1	composition					1925:1935	the predicted sugar monomer composition	1897:1935	the predicted sugar monomer composition derived from in silico genome analysis of the respective gum-like clusters	1897:2010	The EPS secreted by K. baliensis are composed of glucose, galactose and mannose, respectively, which is in agreement with the predicted sugar monomer composition derived from in silico genome analysis of the respective gum-like clusters.
27716345	2	13	theme	molecular	284:292	arg1	levan					301:305	ultra-high molecular weight levan	273:305	ultra-high molecular weight levan from sucrose	273:318	Kozakia (K.) baliensis is a relatively new member of AAB, which produces ultra-high molecular weight levan from sucrose.
27716345	13	14	dep	relevant	2488:2495	arg1	unique					2498:2503	unique	2498:2503	unique	2498:2503	Consequently, the present study provides the basis for establishment of K. baliensis strains as novel microbial cell factories for biotechnologically relevant, unique polysaccharides.
27716345	13	15	theme	novel	2434:2438	arg1	factories					2455:2463	novel microbial cell factories	2434:2463	novel microbial cell factories	2434:2463	Consequently, the present study provides the basis for establishment of K. baliensis strains as novel microbial cell factories for biotechnologically relevant, unique polysaccharides.
27716345	5	16	theme	forming	784:790	arg1	clusters					792:799	typical EPS forming clusters	772:799	typical EPS forming clusters	772:799	RESULTS By whole genome sequencing of both strains, circularized genomes could be established and typical EPS forming clusters were identified.
27716345	9	17	theme	essential	1687:1695	arg1	role					1697:1700	the essential role	1683:1700	the essential role of gumD and of the associated gum genes for production of these new EPS	1683:1772	A mutant strain of K. baliensis NBRC 16680 lacking EPS production on sucrose-deficient media exhibited a transposon insertion in front of the gumD gene of its gum-like cluster in contrast to the wildtype strain, which indicated the essential role of gumD and of the associated gum genes for production of these new EPS.
27716345	12	18	theme	HePS	2279:2282	arg1	biosynthesis					2251:2262	the biosynthesis	2247:2262	the biosynthesis of these novel HePS	2247:2282	Via genome sequencing of K. baliensis DSM 14400 + NBRC 16680 we got first insights into the biosynthesis of these novel HePS, which is related to xanthan and acetan biosynthesis.
27716345	4	19	theme	Kozakia	592:598	arg1	strains					600:606	both Kozakia strains	587:606	both Kozakia strains	587:606	This indicated that both Kozakia strains additionally produce new classes of so far not characterized EPS.
27716345	7	20	theme	operons	1020:1026	arg1	fragments					984:992	fragments	984:992	fragments of truncated levansucrase operons	984:1026	In K. baliensis DSM 14400 plasmid encoded cellulose synthase genes and fragments of truncated levansucrase operons could be assigned in contrast to K. baliensis NBRC 16680.
27716345	11	21	theme	K.	2112:2113	arg1	baliensis					2115:2123	K. baliensis	2112:2123	K. baliensis	2112:2123	CONCLUSIONS By comparative sugar monomer and genome analysis, the polymeric substances secreted by K. baliensis can be considered as unique HePS.
27716345	7	22	theme	synthase	965:972	arg1	plasmid					939:945	DSM 14400 plasmid	929:945	DSM 14400 plasmid	929:945	In K. baliensis DSM 14400 plasmid encoded cellulose synthase genes and fragments of truncated levansucrase operons could be assigned in contrast to K. baliensis NBRC 16680.
27716345	7	22	theme	synthase	965:972	arg1	genes					974:978	cellulose synthase genes	955:978	cellulose synthase genes	955:978	In K. baliensis DSM 14400 plasmid encoded cellulose synthase genes and fragments of truncated levansucrase operons could be assigned in contrast to K. baliensis NBRC 16680.
27716345	4	23	theme	characterized	655:667	arg1	EPS					669:671	so far not characterized EPS	644:671	so far not characterized EPS	644:671	This indicated that both Kozakia strains additionally produce new classes of so far not characterized EPS.
27716345	7	24	theme	truncated	997:1005	arg1	operons					1020:1026	truncated levansucrase operons	997:1026	truncated levansucrase operons	997:1026	In K. baliensis DSM 14400 plasmid encoded cellulose synthase genes and fragments of truncated levansucrase operons could be assigned in contrast to K. baliensis NBRC 16680.
27716345	2	25	theme	K.	209:210	arg1	baliensis					213:221	Kozakia (K.) baliensis	200:221	Kozakia (K.) baliensis	200:221	Kozakia (K.) baliensis is a relatively new member of AAB, which produces ultra-high molecular weight levan from sucrose.
27716345	2	25	theme	K.	209:210	arg1	member					243:248	a relatively new member	226:248	a relatively new member	226:248	Kozakia (K.) baliensis is a relatively new member of AAB, which produces ultra-high molecular weight levan from sucrose.
27716345	9	26	theme	mutant	1457:1462	arg1	strain					1464:1469	A mutant strain	1455:1469	A mutant strain of K. baliensis NBRC 16680 lacking EPS production on sucrose-deficient media	1455:1546	A mutant strain of K. baliensis NBRC 16680 lacking EPS production on sucrose-deficient media exhibited a transposon insertion in front of the gumD gene of its gum-like cluster in contrast to the wildtype strain, which indicated the essential role of gumD and of the associated gum genes for production of these new EPS.
27716345	1	27	theme	known	114:118	arg1	producers					120:128	well known producers	109:128	well known producers	109:128	BACKGROUND Acetic acid bacteria (AAB) are well known producers of commercially used exopolysaccharides, such as cellulose and levan.
27716345	1	27	theme	known	114:118	arg1	cellulose					179:187	cellulose	179:187	cellulose	179:187	BACKGROUND Acetic acid bacteria (AAB) are well known producers of commercially used exopolysaccharides, such as cellulose and levan.
27716345	1	27	theme	known	114:118	arg1	bacteria					90:97	BACKGROUND Acetic acid bacteria	67:97	BACKGROUND Acetic acid bacteria (AAB)	67:103	BACKGROUND Acetic acid bacteria (AAB) are well known producers of commercially used exopolysaccharides, such as cellulose and levan.
27716345	1	27	theme	known	114:118	arg1	levan					193:197	levan	193:197	levan	193:197	BACKGROUND Acetic acid bacteria (AAB) are well known producers of commercially used exopolysaccharides, such as cellulose and levan.
27716345	11	28	theme	comparative	2028:2038	arg1	monomer					2046:2052	comparative sugar monomer and genome analysis	2028:2072	monomer	2046:2052	CONCLUSIONS By comparative sugar monomer and genome analysis, the polymeric substances secreted by K. baliensis can be considered as unique HePS.
27716345	10	29	dep	in	1950:1951	arg1	silico					1953:1958	silico	1953:1958	silico	1953:1958	The EPS secreted by K. baliensis are composed of glucose, galactose and mannose, respectively, which is in agreement with the predicted sugar monomer composition derived from in silico genome analysis of the respective gum-like clusters.
27716345	3	30	from	mannitol	519:526	arg1	sources					558:564	(main) carbon sources	544:564	(main) carbon sources	544:564	Throughout cultivation of two K. baliensis strains (DSM 14400, NBRC 16680) on sucrose-deficient media, we found that both strains still produce high amounts of mucous, water-soluble substances from mannitol and glycerol as (main) carbon sources.
27716345	3	30	from	mannitol	519:526	arg1	substances					503:512	mucous, water-soluble substances	481:512	mucous, water-soluble substances from mannitol and glycerol	481:539	Throughout cultivation of two K. baliensis strains (DSM 14400, NBRC 16680) on sucrose-deficient media, we found that both strains still produce high amounts of mucous, water-soluble substances from mannitol and glycerol as (main) carbon sources.
27716345	3	30	from	mannitol	519:526	arg1	amounts					470:476	high amounts	465:476	high amounts of mucous, water-soluble substances from mannitol and glycerol	465:539	Throughout cultivation of two K. baliensis strains (DSM 14400, NBRC 16680) on sucrose-deficient media, we found that both strains still produce high amounts of mucous, water-soluble substances from mannitol and glycerol as (main) carbon sources.
27716345	13	31	theme	baliensis	2413:2421	arg1	strains					2423:2429	K. baliensis strains	2410:2429	K. baliensis strains as novel microbial cell factories	2410:2463	Consequently, the present study provides the basis for establishment of K. baliensis strains as novel microbial cell factories for biotechnologically relevant, unique polysaccharides.
27716345	2	32	theme	new	239:241	arg1	baliensis					213:221	Kozakia (K.) baliensis	200:221	Kozakia (K.) baliensis	200:221	Kozakia (K.) baliensis is a relatively new member of AAB, which produces ultra-high molecular weight levan from sucrose.
27716345	2	32	theme	new	239:241	arg1	member					243:248	a relatively new member	226:248	a relatively new member	226:248	Kozakia (K.) baliensis is a relatively new member of AAB, which produces ultra-high molecular weight levan from sucrose.
27716345	3	33	from	glycerol	532:539	arg1	sources					558:564	(main) carbon sources	544:564	(main) carbon sources	544:564	Throughout cultivation of two K. baliensis strains (DSM 14400, NBRC 16680) on sucrose-deficient media, we found that both strains still produce high amounts of mucous, water-soluble substances from mannitol and glycerol as (main) carbon sources.
27716345	3	33	from	glycerol	532:539	arg1	substances					503:512	mucous, water-soluble substances	481:512	mucous, water-soluble substances from mannitol and glycerol	481:539	Throughout cultivation of two K. baliensis strains (DSM 14400, NBRC 16680) on sucrose-deficient media, we found that both strains still produce high amounts of mucous, water-soluble substances from mannitol and glycerol as (main) carbon sources.
27716345	3	33	from	glycerol	532:539	arg1	amounts					470:476	high amounts	465:476	high amounts of mucous, water-soluble substances from mannitol and glycerol	465:539	Throughout cultivation of two K. baliensis strains (DSM 14400, NBRC 16680) on sucrose-deficient media, we found that both strains still produce high amounts of mucous, water-soluble substances from mannitol and glycerol as (main) carbon sources.
27716345	0	34	theme	exopolysaccharide	14:30	arg1	biosynthesis					32:43	exopolysaccharide biosynthesis	14:43	exopolysaccharide biosynthesis	14:43	Dissection of exopolysaccharide biosynthesis in Kozakia baliensis.
27716345	1	35	theme	used	146:149	arg1	exopolysaccharides					151:168	commercially used exopolysaccharides	133:168	commercially used exopolysaccharides	133:168	BACKGROUND Acetic acid bacteria (AAB) are well known producers of commercially used exopolysaccharides, such as cellulose and levan.
27716345	9	36	theme	gum	1732:1734	arg1	genes					1736:1740	the associated gum genes	1717:1740	the associated gum genes	1717:1740	A mutant strain of K. baliensis NBRC 16680 lacking EPS production on sucrose-deficient media exhibited a transposon insertion in front of the gumD gene of its gum-like cluster in contrast to the wildtype strain, which indicated the essential role of gumD and of the associated gum genes for production of these new EPS.
27716345	12	37	theme	first	2227:2231	arg1	insights					2233:2240	first insights	2227:2240	first insights	2227:2240	Via genome sequencing of K. baliensis DSM 14400 + NBRC 16680 we got first insights into the biosynthesis of these novel HePS, which is related to xanthan and acetan biosynthesis.
27716345	10	38	theme	gum-like	1994:2001	arg1	clusters					2003:2010	the respective gum-like clusters	1979:2010	the respective gum-like clusters	1979:2010	The EPS secreted by K. baliensis are composed of glucose, galactose and mannose, respectively, which is in agreement with the predicted sugar monomer composition derived from in silico genome analysis of the respective gum-like clusters.
27716345	5	39	theme	strains	717:723	arg1	sequencing					698:707	whole genome sequencing	685:707	whole genome sequencing of both strains	685:723	RESULTS By whole genome sequencing of both strains, circularized genomes could be established and typical EPS forming clusters were identified.
27716345	0	40	from	Dissection	0:9	arg1	baliensis					56:64	Kozakia baliensis	48:64	Kozakia baliensis	48:64	Dissection of exopolysaccharide biosynthesis in Kozakia baliensis.
27716345	3	41	dep	strains	364:370	arg1	14400					377:381	DSM 14400	373:381	DSM 14400	373:381	Throughout cultivation of two K. baliensis strains (DSM 14400, NBRC 16680) on sucrose-deficient media, we found that both strains still produce high amounts of mucous, water-soluble substances from mannitol and glycerol as (main) carbon sources.
27716345	3	41	dep	strains	364:370	arg1	NBRC					384:387	NBRC 16680	384:393	NBRC 16680	384:393	Throughout cultivation of two K. baliensis strains (DSM 14400, NBRC 16680) on sucrose-deficient media, we found that both strains still produce high amounts of mucous, water-soluble substances from mannitol and glycerol as (main) carbon sources.
27716345	8	42	theme	gum-like	1306:1313	arg1	clusters					1343:1350	gum-like heteropolysaccharide (HePS) clusters	1306:1350	gum-like heteropolysaccharide (HePS) clusters from other acetic acid bacteria such as Gluconacetobacter diazotrophicus and Komagataeibacter xylinus	1306:1452	Additionally, both K. baliensis strains harbor identical gum-like clusters, which are related to the well characterized gum cluster coding for xanthan synthesis in Xanthomanas campestris and show highest similarity with gum-like heteropolysaccharide (HePS) clusters from other acetic acid bacteria such as Gluconacetobacter diazotrophicus and Komagataeibacter xylinus.
27716345	1	43	theme	Acetic	78:83	arg1	producers					120:128	well known producers	109:128	well known producers	109:128	BACKGROUND Acetic acid bacteria (AAB) are well known producers of commercially used exopolysaccharides, such as cellulose and levan.
27716345	1	43	theme	Acetic	78:83	arg1	AAB					100:102	AAB	100:102	AAB	100:102	BACKGROUND Acetic acid bacteria (AAB) are well known producers of commercially used exopolysaccharides, such as cellulose and levan.
27716345	1	43	theme	Acetic	78:83	arg1	cellulose					179:187	cellulose	179:187	cellulose	179:187	BACKGROUND Acetic acid bacteria (AAB) are well known producers of commercially used exopolysaccharides, such as cellulose and levan.
27716345	1	43	theme	Acetic	78:83	arg1	bacteria					90:97	BACKGROUND Acetic acid bacteria	67:97	BACKGROUND Acetic acid bacteria (AAB)	67:103	BACKGROUND Acetic acid bacteria (AAB) are well known producers of commercially used exopolysaccharides, such as cellulose and levan.
27716345	1	43	theme	Acetic	78:83	arg1	levan					193:197	levan	193:197	levan	193:197	BACKGROUND Acetic acid bacteria (AAB) are well known producers of commercially used exopolysaccharides, such as cellulose and levan.
27716345	12	44	theme	14400 + NBRC	2201:2212	arg1	sequencing					2170:2179	genome sequencing	2163:2179	genome sequencing of K. baliensis DSM 14400 + NBRC 16680	2163:2218	Via genome sequencing of K. baliensis DSM 14400 + NBRC 16680 we got first insights into the biosynthesis of these novel HePS, which is related to xanthan and acetan biosynthesis.
27716345	12	45	theme	DSM	2197:2199	arg1	14400 + NBRC					2201:2212	K. baliensis DSM 14400 + NBRC 16680	2184:2218	K. baliensis DSM 14400 + NBRC 16680	2184:2218	Via genome sequencing of K. baliensis DSM 14400 + NBRC 16680 we got first insights into the biosynthesis of these novel HePS, which is related to xanthan and acetan biosynthesis.
27716345	5	46	theme	genome	691:696	arg1	sequencing					698:707	whole genome sequencing	685:707	whole genome sequencing of both strains	685:723	RESULTS By whole genome sequencing of both strains, circularized genomes could be established and typical EPS forming clusters were identified.
27716345	3	47	from	strains	364:370	arg1	media					417:421	sucrose-deficient media	399:421	sucrose-deficient media	399:421	Throughout cultivation of two K. baliensis strains (DSM 14400, NBRC 16680) on sucrose-deficient media, we found that both strains still produce high amounts of mucous, water-soluble substances from mannitol and glycerol as (main) carbon sources.
27716345	3	48	theme	strains	364:370	arg1	cultivation					332:342	cultivation	332:342	cultivation of two K. baliensis strains (DSM 14400, NBRC 16680) on sucrose-deficient media	332:421	Throughout cultivation of two K. baliensis strains (DSM 14400, NBRC 16680) on sucrose-deficient media, we found that both strains still produce high amounts of mucous, water-soluble substances from mannitol and glycerol as (main) carbon sources.
27716345	3	49	theme	K.	351:352	arg1	strains					364:370	two K. baliensis strains	347:370	two K. baliensis strains (DSM 14400, NBRC 16680) on sucrose-deficient media	347:421	Throughout cultivation of two K. baliensis strains (DSM 14400, NBRC 16680) on sucrose-deficient media, we found that both strains still produce high amounts of mucous, water-soluble substances from mannitol and glycerol as (main) carbon sources.
27716345	5	50	theme	typical	772:778	arg1	clusters					792:799	typical EPS forming clusters	772:799	typical EPS forming clusters	772:799	RESULTS By whole genome sequencing of both strains, circularized genomes could be established and typical EPS forming clusters were identified.
27716345	2	51	theme	AAB	253:255	arg1	baliensis					213:221	Kozakia (K.) baliensis	200:221	Kozakia (K.) baliensis	200:221	Kozakia (K.) baliensis is a relatively new member of AAB, which produces ultra-high molecular weight levan from sucrose.
27716345	2	51	theme	AAB	253:255	arg1	member					243:248	a relatively new member	226:248	a relatively new member	226:248	Kozakia (K.) baliensis is a relatively new member of AAB, which produces ultra-high molecular weight levan from sucrose.
27716345	11	52	theme	unique	2146:2151	arg1	substances					2089:2098	the polymeric substances	2075:2098	the polymeric substances secreted by K. baliensis	2075:2123	CONCLUSIONS By comparative sugar monomer and genome analysis, the polymeric substances secreted by K. baliensis can be considered as unique HePS.
27716345	11	52	theme	unique	2146:2151	arg1	HePS					2153:2156	unique HePS	2146:2156	unique HePS	2146:2156	CONCLUSIONS By comparative sugar monomer and genome analysis, the polymeric substances secreted by K. baliensis can be considered as unique HePS.
27716345	8	53	theme	acetic	1363:1368	arg1	xylinus					1446:1452	Komagataeibacter xylinus	1429:1452	Komagataeibacter xylinus	1429:1452	Additionally, both K. baliensis strains harbor identical gum-like clusters, which are related to the well characterized gum cluster coding for xanthan synthesis in Xanthomanas campestris and show highest similarity with gum-like heteropolysaccharide (HePS) clusters from other acetic acid bacteria such as Gluconacetobacter diazotrophicus and Komagataeibacter xylinus.
27716345	8	53	theme	acetic	1363:1368	arg1	bacteria					1375:1382	other acetic acid bacteria	1357:1382	other acetic acid bacteria such as Gluconacetobacter diazotrophicus and Komagataeibacter xylinus	1357:1452	Additionally, both K. baliensis strains harbor identical gum-like clusters, which are related to the well characterized gum cluster coding for xanthan synthesis in Xanthomanas campestris and show highest similarity with gum-like heteropolysaccharide (HePS) clusters from other acetic acid bacteria such as Gluconacetobacter diazotrophicus and Komagataeibacter xylinus.
27716345	8	53	theme	acetic	1363:1368	arg1	diazotrophicus					1410:1423	Gluconacetobacter diazotrophicus	1392:1423	Gluconacetobacter diazotrophicus	1392:1423	Additionally, both K. baliensis strains harbor identical gum-like clusters, which are related to the well characterized gum cluster coding for xanthan synthesis in Xanthomanas campestris and show highest similarity with gum-like heteropolysaccharide (HePS) clusters from other acetic acid bacteria such as Gluconacetobacter diazotrophicus and Komagataeibacter xylinus.
27716345	5	54	theme	circularized	726:737	arg1	genomes					739:745	circularized genomes	726:745	circularized genomes	726:745	RESULTS By whole genome sequencing of both strains, circularized genomes could be established and typical EPS forming clusters were identified.
27716345	10	55	attach	derived	1937:1943	arg2	composition					1925:1935	the predicted sugar monomer composition	1897:1935	the predicted sugar monomer composition derived from in silico genome analysis of the respective gum-like clusters	1897:2010	The EPS secreted by K. baliensis are composed of glucose, galactose and mannose, respectively, which is in agreement with the predicted sugar monomer composition derived from in silico genome analysis of the respective gum-like clusters.
27716345	10	55	attach	derived	1937:1943	arg1	analysis					1967:1974	in silico genome analysis	1950:1974	in silico genome analysis of the respective gum-like clusters	1950:2010	The EPS secreted by K. baliensis are composed of glucose, galactose and mannose, respectively, which is in agreement with the predicted sugar monomer composition derived from in silico genome analysis of the respective gum-like clusters.
27716345	9	56	from	strain	1464:1469	arg1	media					1542:1546	sucrose-deficient media	1524:1546	sucrose-deficient media	1524:1546	A mutant strain of K. baliensis NBRC 16680 lacking EPS production on sucrose-deficient media exhibited a transposon insertion in front of the gumD gene of its gum-like cluster in contrast to the wildtype strain, which indicated the essential role of gumD and of the associated gum genes for production of these new EPS.
27716345	3	57	theme	DSM	373:375	arg1	14400					377:381	DSM 14400	373:381	DSM 14400	373:381	Throughout cultivation of two K. baliensis strains (DSM 14400, NBRC 16680) on sucrose-deficient media, we found that both strains still produce high amounts of mucous, water-soluble substances from mannitol and glycerol as (main) carbon sources.
27716345	3	57	theme	DSM	373:375	arg1	NBRC					384:387	NBRC 16680	384:393	NBRC 16680	384:393	Throughout cultivation of two K. baliensis strains (DSM 14400, NBRC 16680) on sucrose-deficient media, we found that both strains still produce high amounts of mucous, water-soluble substances from mannitol and glycerol as (main) carbon sources.
27716345	6	58	located	detected	879:886	arg1	strains					904:910	both Kozakia strains	891:910	both Kozakia strains	891:910	As expected, complete ORFs coding for levansucrases could be detected in both Kozakia strains.
27716345	6	58	located	detected	879:886	arg2	ORFs					840:843	complete ORFs	831:843	complete ORFs coding for levansucrases	831:868	As expected, complete ORFs coding for levansucrases could be detected in both Kozakia strains.
27716345	9	59	dep	strain	1659:1664	arg1	contrast					1634:1641	contrast	1634:1641	contrast	1634:1641	A mutant strain of K. baliensis NBRC 16680 lacking EPS production on sucrose-deficient media exhibited a transposon insertion in front of the gumD gene of its gum-like cluster in contrast to the wildtype strain, which indicated the essential role of gumD and of the associated gum genes for production of these new EPS.
27716345	2	60	theme	Kozakia	200:206	arg1	baliensis					213:221	Kozakia (K.) baliensis	200:221	Kozakia (K.) baliensis	200:221	Kozakia (K.) baliensis is a relatively new member of AAB, which produces ultra-high molecular weight levan from sucrose.
27716345	2	60	theme	Kozakia	200:206	arg1	member					243:248	a relatively new member	226:248	a relatively new member	226:248	Kozakia (K.) baliensis is a relatively new member of AAB, which produces ultra-high molecular weight levan from sucrose.
27716345	8	61	dep	Xanthomanas	1250:1260	arg1	campestris					1262:1271	Xanthomanas campestris	1250:1271	Xanthomanas campestris	1250:1271	Additionally, both K. baliensis strains harbor identical gum-like clusters, which are related to the well characterized gum cluster coding for xanthan synthesis in Xanthomanas campestris and show highest similarity with gum-like heteropolysaccharide (HePS) clusters from other acetic acid bacteria such as Gluconacetobacter diazotrophicus and Komagataeibacter xylinus.
27716345	9	62	theme	EPS	1506:1508	arg1	production					1510:1519	EPS production	1506:1519	EPS production	1506:1519	A mutant strain of K. baliensis NBRC 16680 lacking EPS production on sucrose-deficient media exhibited a transposon insertion in front of the gumD gene of its gum-like cluster in contrast to the wildtype strain, which indicated the essential role of gumD and of the associated gum genes for production of these new EPS.
27716345	12	63	theme	acetan	2317:2322	arg1	biosynthesis					2324:2335	acetan biosynthesis	2317:2335	acetan biosynthesis	2317:2335	Via genome sequencing of K. baliensis DSM 14400 + NBRC 16680 we got first insights into the biosynthesis of these novel HePS, which is related to xanthan and acetan biosynthesis.
27716345	9	64	theme	cluster	1623:1629	arg1	insertion					1571:1579	a transposon insertion	1558:1579	a transposon insertion in front of the gumD gene of its gum-like cluster	1558:1629	A mutant strain of K. baliensis NBRC 16680 lacking EPS production on sucrose-deficient media exhibited a transposon insertion in front of the gumD gene of its gum-like cluster in contrast to the wildtype strain, which indicated the essential role of gumD and of the associated gum genes for production of these new EPS.
27716345	11	65	dep	CONCLUSIONS	2013:2023	arg1	considered					2132:2141	considered	2132:2141	can be considered as unique HePS	2125:2156	CONCLUSIONS By comparative sugar monomer and genome analysis, the polymeric substances secreted by K. baliensis can be considered as unique HePS.
27716345	13	66	theme	relevant	2488:2495	arg1	polysaccharides					2505:2519	biotechnologically relevant, unique polysaccharides	2469:2519	biotechnologically relevant, unique polysaccharides	2469:2519	Consequently, the present study provides the basis for establishment of K. baliensis strains as novel microbial cell factories for biotechnologically relevant, unique polysaccharides.
27716345	7	67	theme	K.	1061:1062	arg1	NBRC					1074:1077	K. baliensis NBRC 16680	1061:1083	K. baliensis NBRC 16680	1061:1083	In K. baliensis DSM 14400 plasmid encoded cellulose synthase genes and fragments of truncated levansucrase operons could be assigned in contrast to K. baliensis NBRC 16680.
27716345	8	68	theme	K.	1105:1106	arg1	strains					1118:1124	both K. baliensis strains	1100:1124	both K. baliensis strains	1100:1124	Additionally, both K. baliensis strains harbor identical gum-like clusters, which are related to the well characterized gum cluster coding for xanthan synthesis in Xanthomanas campestris and show highest similarity with gum-like heteropolysaccharide (HePS) clusters from other acetic acid bacteria such as Gluconacetobacter diazotrophicus and Komagataeibacter xylinus.
27716345	13	69	theme	cell	2450:2453	arg1	factories					2455:2463	novel microbial cell factories	2434:2463	novel microbial cell factories	2434:2463	Consequently, the present study provides the basis for establishment of K. baliensis strains as novel microbial cell factories for biotechnologically relevant, unique polysaccharides.
27716345	3	70	theme	mucous	481:486	arg1	substances					503:512	mucous, water-soluble substances	481:512	mucous, water-soluble substances from mannitol and glycerol	481:539	Throughout cultivation of two K. baliensis strains (DSM 14400, NBRC 16680) on sucrose-deficient media, we found that both strains still produce high amounts of mucous, water-soluble substances from mannitol and glycerol as (main) carbon sources.
27716345	4	71	theme	new	629:631	arg1	classes					633:639	new classes	629:639	new classes of so far not characterized EPS	629:671	This indicated that both Kozakia strains additionally produce new classes of so far not characterized EPS.
27716345	6	72	theme	complete	831:838	arg1	ORFs					840:843	complete ORFs	831:843	complete ORFs coding for levansucrases	831:868	As expected, complete ORFs coding for levansucrases could be detected in both Kozakia strains.
27716345	2	73	theme	weight	294:299	arg1	levan					301:305	ultra-high molecular weight levan	273:305	ultra-high molecular weight levan from sucrose	273:318	Kozakia (K.) baliensis is a relatively new member of AAB, which produces ultra-high molecular weight levan from sucrose.
27716345	8	74	theme	identical	1133:1141	arg1	clusters					1152:1159	identical gum-like clusters	1133:1159	identical gum-like clusters	1133:1159	Additionally, both K. baliensis strains harbor identical gum-like clusters, which are related to the well characterized gum cluster coding for xanthan synthesis in Xanthomanas campestris and show highest similarity with gum-like heteropolysaccharide (HePS) clusters from other acetic acid bacteria such as Gluconacetobacter diazotrophicus and Komagataeibacter xylinus.
27716345	8	75	theme	characterized	1192:1204	arg1	cluster					1210:1216	the well characterized gum cluster	1183:1216	the well characterized gum cluster coding for xanthan synthesis in Xanthomanas campestris	1183:1271	Additionally, both K. baliensis strains harbor identical gum-like clusters, which are related to the well characterized gum cluster coding for xanthan synthesis in Xanthomanas campestris and show highest similarity with gum-like heteropolysaccharide (HePS) clusters from other acetic acid bacteria such as Gluconacetobacter diazotrophicus and Komagataeibacter xylinus.
27716345	10	76	theme	predicted	1901:1909	arg1	composition					1925:1935	the predicted sugar monomer composition	1897:1935	the predicted sugar monomer composition derived from in silico genome analysis of the respective gum-like clusters	1897:2010	The EPS secreted by K. baliensis are composed of glucose, galactose and mannose, respectively, which is in agreement with the predicted sugar monomer composition derived from in silico genome analysis of the respective gum-like clusters.
27716345	13	77	theme	strains	2423:2429	arg1	establishment					2393:2405	establishment	2393:2405	establishment of K. baliensis strains as novel microbial cell factories	2393:2463	Consequently, the present study provides the basis for establishment of K. baliensis strains as novel microbial cell factories for biotechnologically relevant, unique polysaccharides.
27716345	2	78	theme	ultra-high	273:282	arg1	levan					301:305	ultra-high molecular weight levan	273:305	ultra-high molecular weight levan from sucrose	273:318	Kozakia (K.) baliensis is a relatively new member of AAB, which produces ultra-high molecular weight levan from sucrose.
27716345	5	79	theme	EPS	780:782	arg1	clusters					792:799	typical EPS forming clusters	772:799	typical EPS forming clusters	772:799	RESULTS By whole genome sequencing of both strains, circularized genomes could be established and typical EPS forming clusters were identified.
27716345	8	80	theme	Komagataeibacter	1429:1444	arg1	xylinus					1446:1452	Komagataeibacter xylinus	1429:1452	Komagataeibacter xylinus	1429:1452	Additionally, both K. baliensis strains harbor identical gum-like clusters, which are related to the well characterized gum cluster coding for xanthan synthesis in Xanthomanas campestris and show highest similarity with gum-like heteropolysaccharide (HePS) clusters from other acetic acid bacteria such as Gluconacetobacter diazotrophicus and Komagataeibacter xylinus.
27716345	7	81	theme	levansucrase	1007:1018	arg1	operons					1020:1026	truncated levansucrase operons	997:1026	truncated levansucrase operons	997:1026	In K. baliensis DSM 14400 plasmid encoded cellulose synthase genes and fragments of truncated levansucrase operons could be assigned in contrast to K. baliensis NBRC 16680.
27716345	3	82	from	cultivation	332:342	arg1	media					417:421	sucrose-deficient media	399:421	sucrose-deficient media	399:421	Throughout cultivation of two K. baliensis strains (DSM 14400, NBRC 16680) on sucrose-deficient media, we found that both strains still produce high amounts of mucous, water-soluble substances from mannitol and glycerol as (main) carbon sources.
27716345	3	83	theme	main	545:548	arg1	sources					558:564	(main) carbon sources	544:564	(main) carbon sources	544:564	Throughout cultivation of two K. baliensis strains (DSM 14400, NBRC 16680) on sucrose-deficient media, we found that both strains still produce high amounts of mucous, water-soluble substances from mannitol and glycerol as (main) carbon sources.
27716345	3	83	theme	main	545:548	arg1	substances					503:512	mucous, water-soluble substances	481:512	mucous, water-soluble substances from mannitol and glycerol	481:539	Throughout cultivation of two K. baliensis strains (DSM 14400, NBRC 16680) on sucrose-deficient media, we found that both strains still produce high amounts of mucous, water-soluble substances from mannitol and glycerol as (main) carbon sources.
27716345	3	83	theme	main	545:548	arg1	amounts					470:476	high amounts	465:476	high amounts of mucous, water-soluble substances from mannitol and glycerol	465:539	Throughout cultivation of two K. baliensis strains (DSM 14400, NBRC 16680) on sucrose-deficient media, we found that both strains still produce high amounts of mucous, water-soluble substances from mannitol and glycerol as (main) carbon sources.
27716345	6	84	theme	Kozakia	896:902	arg1	strains					904:910	both Kozakia strains	891:910	both Kozakia strains	891:910	As expected, complete ORFs coding for levansucrases could be detected in both Kozakia strains.
27716345	3	85	dep	mucous	481:486	arg1	water-soluble					489:501	water-soluble	489:501	water-soluble	489:501	Throughout cultivation of two K. baliensis strains (DSM 14400, NBRC 16680) on sucrose-deficient media, we found that both strains still produce high amounts of mucous, water-soluble substances from mannitol and glycerol as (main) carbon sources.
27716345	9	86	theme	gumD	1705:1708	arg1	role					1697:1700	the essential role	1683:1700	the essential role of gumD and of the associated gum genes for production of these new EPS	1683:1772	A mutant strain of K. baliensis NBRC 16680 lacking EPS production on sucrose-deficient media exhibited a transposon insertion in front of the gumD gene of its gum-like cluster in contrast to the wildtype strain, which indicated the essential role of gumD and of the associated gum genes for production of these new EPS.
27716345	10	87	theme	monomer	1917:1923	arg1	composition					1925:1935	the predicted sugar monomer composition	1897:1935	the predicted sugar monomer composition derived from in silico genome analysis of the respective gum-like clusters	1897:2010	The EPS secreted by K. baliensis are composed of glucose, galactose and mannose, respectively, which is in agreement with the predicted sugar monomer composition derived from in silico genome analysis of the respective gum-like clusters.
27716345	13	88	theme	K.	2410:2411	arg1	strains					2423:2429	K. baliensis strains	2410:2429	K. baliensis strains as novel microbial cell factories	2410:2463	Consequently, the present study provides the basis for establishment of K. baliensis strains as novel microbial cell factories for biotechnologically relevant, unique polysaccharides.
27716345	10	89	theme	K.	1795:1796	arg1	baliensis					1798:1806	K. baliensis	1795:1806	K. baliensis	1795:1806	The EPS secreted by K. baliensis are composed of glucose, galactose and mannose, respectively, which is in agreement with the predicted sugar monomer composition derived from in silico genome analysis of the respective gum-like clusters.
27716345	7	90	theme	cellulose	955:963	arg1	plasmid					939:945	DSM 14400 plasmid	929:945	DSM 14400 plasmid	929:945	In K. baliensis DSM 14400 plasmid encoded cellulose synthase genes and fragments of truncated levansucrase operons could be assigned in contrast to K. baliensis NBRC 16680.
27716345	7	90	theme	cellulose	955:963	arg1	genes					974:978	cellulose synthase genes	955:978	cellulose synthase genes	955:978	In K. baliensis DSM 14400 plasmid encoded cellulose synthase genes and fragments of truncated levansucrase operons could be assigned in contrast to K. baliensis NBRC 16680.
27716345	9	91	theme	NBRC	1487:1490	arg1	strain					1464:1469	A mutant strain	1455:1469	A mutant strain of K. baliensis NBRC 16680 lacking EPS production on sucrose-deficient media	1455:1546	A mutant strain of K. baliensis NBRC 16680 lacking EPS production on sucrose-deficient media exhibited a transposon insertion in front of the gumD gene of its gum-like cluster in contrast to the wildtype strain, which indicated the essential role of gumD and of the associated gum genes for production of these new EPS.
27716345	11	92	theme	sugar	2040:2044	arg1	monomer					2046:2052	comparative sugar monomer and genome analysis	2028:2072	monomer	2046:2052	CONCLUSIONS By comparative sugar monomer and genome analysis, the polymeric substances secreted by K. baliensis can be considered as unique HePS.
27716345	12	93	theme	novel	2273:2277	arg1	HePS					2279:2282	these novel HePS	2267:2282	these novel HePS	2267:2282	Via genome sequencing of K. baliensis DSM 14400 + NBRC 16680 we got first insights into the biosynthesis of these novel HePS, which is related to xanthan and acetan biosynthesis.
27716345	9	94	theme	sucrose-deficient	1524:1540	arg1	media					1542:1546	sucrose-deficient media	1524:1546	sucrose-deficient media	1524:1546	A mutant strain of K. baliensis NBRC 16680 lacking EPS production on sucrose-deficient media exhibited a transposon insertion in front of the gumD gene of its gum-like cluster in contrast to the wildtype strain, which indicated the essential role of gumD and of the associated gum genes for production of these new EPS.
27716345	4	95	theme	EPS	669:671	arg1	classes					633:639	new classes	629:639	new classes of so far not characterized EPS	629:671	This indicated that both Kozakia strains additionally produce new classes of so far not characterized EPS.
27716345	10	96	theme	genome	1960:1965	arg1	analysis					1967:1974	in silico genome analysis	1950:1974	in silico genome analysis of the respective gum-like clusters	1950:2010	The EPS secreted by K. baliensis are composed of glucose, galactose and mannose, respectively, which is in agreement with the predicted sugar monomer composition derived from in silico genome analysis of the respective gum-like clusters.
27716345	3	97	theme	carbon	551:556	arg1	sources					558:564	(main) carbon sources	544:564	(main) carbon sources	544:564	Throughout cultivation of two K. baliensis strains (DSM 14400, NBRC 16680) on sucrose-deficient media, we found that both strains still produce high amounts of mucous, water-soluble substances from mannitol and glycerol as (main) carbon sources.
27716345	3	97	theme	carbon	551:556	arg1	substances					503:512	mucous, water-soluble substances	481:512	mucous, water-soluble substances from mannitol and glycerol	481:539	Throughout cultivation of two K. baliensis strains (DSM 14400, NBRC 16680) on sucrose-deficient media, we found that both strains still produce high amounts of mucous, water-soluble substances from mannitol and glycerol as (main) carbon sources.
27716345	3	97	theme	carbon	551:556	arg1	amounts					470:476	high amounts	465:476	high amounts of mucous, water-soluble substances from mannitol and glycerol	465:539	Throughout cultivation of two K. baliensis strains (DSM 14400, NBRC 16680) on sucrose-deficient media, we found that both strains still produce high amounts of mucous, water-soluble substances from mannitol and glycerol as (main) carbon sources.
27716345	9	98	theme	associated	1721:1730	arg1	genes					1736:1740	the associated gum genes	1717:1740	the associated gum genes	1717:1740	A mutant strain of K. baliensis NBRC 16680 lacking EPS production on sucrose-deficient media exhibited a transposon insertion in front of the gumD gene of its gum-like cluster in contrast to the wildtype strain, which indicated the essential role of gumD and of the associated gum genes for production of these new EPS.
27716345	10	99	theme	in	1950:1951	arg1	analysis					1967:1974	in silico genome analysis	1950:1974	in silico genome analysis of the respective gum-like clusters	1950:2010	The EPS secreted by K. baliensis are composed of glucose, galactose and mannose, respectively, which is in agreement with the predicted sugar monomer composition derived from in silico genome analysis of the respective gum-like clusters.
27716345	7	100	theme	DSM	929:931	arg1	plasmid					939:945	DSM 14400 plasmid	929:945	DSM 14400 plasmid	929:945	In K. baliensis DSM 14400 plasmid encoded cellulose synthase genes and fragments of truncated levansucrase operons could be assigned in contrast to K. baliensis NBRC 16680.
27716345	7	100	theme	DSM	929:931	arg1	genes					974:978	cellulose synthase genes	955:978	cellulose synthase genes	955:978	In K. baliensis DSM 14400 plasmid encoded cellulose synthase genes and fragments of truncated levansucrase operons could be assigned in contrast to K. baliensis NBRC 16680.
27716345	1	101	theme	exopolysaccharides	151:168	arg1	producers					120:128	well known producers	109:128	well known producers	109:128	BACKGROUND Acetic acid bacteria (AAB) are well known producers of commercially used exopolysaccharides, such as cellulose and levan.
27716345	1	101	theme	exopolysaccharides	151:168	arg1	cellulose					179:187	cellulose	179:187	cellulose	179:187	BACKGROUND Acetic acid bacteria (AAB) are well known producers of commercially used exopolysaccharides, such as cellulose and levan.
27716345	1	101	theme	exopolysaccharides	151:168	arg1	bacteria					90:97	BACKGROUND Acetic acid bacteria	67:97	BACKGROUND Acetic acid bacteria (AAB)	67:103	BACKGROUND Acetic acid bacteria (AAB) are well known producers of commercially used exopolysaccharides, such as cellulose and levan.
27716345	1	101	theme	exopolysaccharides	151:168	arg1	levan					193:197	levan	193:197	levan	193:197	BACKGROUND Acetic acid bacteria (AAB) are well known producers of commercially used exopolysaccharides, such as cellulose and levan.
27716345	9	102	theme	genes	1736:1740	arg1	role					1697:1700	the essential role	1683:1700	the essential role of gumD and of the associated gum genes for production of these new EPS	1683:1772	A mutant strain of K. baliensis NBRC 16680 lacking EPS production on sucrose-deficient media exhibited a transposon insertion in front of the gumD gene of its gum-like cluster in contrast to the wildtype strain, which indicated the essential role of gumD and of the associated gum genes for production of these new EPS.
27716345	8	103	theme	highest	1282:1288	arg1	similarity					1290:1299	highest similarity	1282:1299	highest similarity with gum-like heteropolysaccharide (HePS) clusters from other acetic acid bacteria such as Gluconacetobacter diazotrophicus and Komagataeibacter xylinus	1282:1452	Additionally, both K. baliensis strains harbor identical gum-like clusters, which are related to the well characterized gum cluster coding for xanthan synthesis in Xanthomanas campestris and show highest similarity with gum-like heteropolysaccharide (HePS) clusters from other acetic acid bacteria such as Gluconacetobacter diazotrophicus and Komagataeibacter xylinus.
27716345	10	104	theme	respective	1983:1992	arg1	clusters					2003:2010	the respective gum-like clusters	1979:2010	the respective gum-like clusters	1979:2010	The EPS secreted by K. baliensis are composed of glucose, galactose and mannose, respectively, which is in agreement with the predicted sugar monomer composition derived from in silico genome analysis of the respective gum-like clusters.
27716345	1	105	theme	BACKGROUND	67:76	arg1	producers					120:128	well known producers	109:128	well known producers	109:128	BACKGROUND Acetic acid bacteria (AAB) are well known producers of commercially used exopolysaccharides, such as cellulose and levan.
27716345	1	105	theme	BACKGROUND	67:76	arg1	AAB					100:102	AAB	100:102	AAB	100:102	BACKGROUND Acetic acid bacteria (AAB) are well known producers of commercially used exopolysaccharides, such as cellulose and levan.
27716345	1	105	theme	BACKGROUND	67:76	arg1	cellulose					179:187	cellulose	179:187	cellulose	179:187	BACKGROUND Acetic acid bacteria (AAB) are well known producers of commercially used exopolysaccharides, such as cellulose and levan.
27716345	1	105	theme	BACKGROUND	67:76	arg1	bacteria					90:97	BACKGROUND Acetic acid bacteria	67:97	BACKGROUND Acetic acid bacteria (AAB)	67:103	BACKGROUND Acetic acid bacteria (AAB) are well known producers of commercially used exopolysaccharides, such as cellulose and levan.
27716345	1	105	theme	BACKGROUND	67:76	arg1	levan					193:197	levan	193:197	levan	193:197	BACKGROUND Acetic acid bacteria (AAB) are well known producers of commercially used exopolysaccharides, such as cellulose and levan.
27716345	12	106	theme	K.	2184:2185	arg1	14400 + NBRC					2201:2212	K. baliensis DSM 14400 + NBRC 16680	2184:2218	K. baliensis DSM 14400 + NBRC 16680	2184:2218	Via genome sequencing of K. baliensis DSM 14400 + NBRC 16680 we got first insights into the biosynthesis of these novel HePS, which is related to xanthan and acetan biosynthesis.
27716345	5	107	theme	whole	685:689	arg1	sequencing					698:707	whole genome sequencing	685:707	whole genome sequencing of both strains	685:723	RESULTS By whole genome sequencing of both strains, circularized genomes could be established and typical EPS forming clusters were identified.
27716345	8	108	from	synthesis	1237:1245	arg1	Xanthomanas					1250:1260	Xanthomanas	1250:1260	Xanthomanas	1250:1260	Additionally, both K. baliensis strains harbor identical gum-like clusters, which are related to the well characterized gum cluster coding for xanthan synthesis in Xanthomanas campestris and show highest similarity with gum-like heteropolysaccharide (HePS) clusters from other acetic acid bacteria such as Gluconacetobacter diazotrophicus and Komagataeibacter xylinus.
27716345	1	109	theme	acid	85:88	arg1	producers					120:128	well known producers	109:128	well known producers	109:128	BACKGROUND Acetic acid bacteria (AAB) are well known producers of commercially used exopolysaccharides, such as cellulose and levan.
27716345	1	109	theme	acid	85:88	arg1	AAB					100:102	AAB	100:102	AAB	100:102	BACKGROUND Acetic acid bacteria (AAB) are well known producers of commercially used exopolysaccharides, such as cellulose and levan.
27716345	1	109	theme	acid	85:88	arg1	cellulose					179:187	cellulose	179:187	cellulose	179:187	BACKGROUND Acetic acid bacteria (AAB) are well known producers of commercially used exopolysaccharides, such as cellulose and levan.
27716345	1	109	theme	acid	85:88	arg1	bacteria					90:97	BACKGROUND Acetic acid bacteria	67:97	BACKGROUND Acetic acid bacteria (AAB)	67:103	BACKGROUND Acetic acid bacteria (AAB) are well known producers of commercially used exopolysaccharides, such as cellulose and levan.
27716345	1	109	theme	acid	85:88	arg1	levan					193:197	levan	193:197	levan	193:197	BACKGROUND Acetic acid bacteria (AAB) are well known producers of commercially used exopolysaccharides, such as cellulose and levan.
27716345	8	110	theme	heteropolysaccharide	1315:1334	arg1	clusters					1343:1350	gum-like heteropolysaccharide (HePS) clusters	1306:1350	gum-like heteropolysaccharide (HePS) clusters from other acetic acid bacteria such as Gluconacetobacter diazotrophicus and Komagataeibacter xylinus	1306:1452	Additionally, both K. baliensis strains harbor identical gum-like clusters, which are related to the well characterized gum cluster coding for xanthan synthesis in Xanthomanas campestris and show highest similarity with gum-like heteropolysaccharide (HePS) clusters from other acetic acid bacteria such as Gluconacetobacter diazotrophicus and Komagataeibacter xylinus.
27716345	3	111	theme	sucrose-deficient	399:415	arg1	media					417:421	sucrose-deficient media	399:421	sucrose-deficient media	399:421	Throughout cultivation of two K. baliensis strains (DSM 14400, NBRC 16680) on sucrose-deficient media, we found that both strains still produce high amounts of mucous, water-soluble substances from mannitol and glycerol as (main) carbon sources.
27716345	9	112	theme	transposon	1560:1569	arg1	insertion					1571:1579	a transposon insertion	1558:1579	a transposon insertion in front of the gumD gene of its gum-like cluster	1558:1629	A mutant strain of K. baliensis NBRC 16680 lacking EPS production on sucrose-deficient media exhibited a transposon insertion in front of the gumD gene of its gum-like cluster in contrast to the wildtype strain, which indicated the essential role of gumD and of the associated gum genes for production of these new EPS.
27716345	9	113	theme	EPS	1770:1772	arg1	production					1746:1755	production	1746:1755	production of these new EPS	1746:1772	A mutant strain of K. baliensis NBRC 16680 lacking EPS production on sucrose-deficient media exhibited a transposon insertion in front of the gumD gene of its gum-like cluster in contrast to the wildtype strain, which indicated the essential role of gumD and of the associated gum genes for production of these new EPS.
27716345	8	114	theme	HePS	1337:1340	arg1	clusters					1343:1350	gum-like heteropolysaccharide (HePS) clusters	1306:1350	gum-like heteropolysaccharide (HePS) clusters from other acetic acid bacteria such as Gluconacetobacter diazotrophicus and Komagataeibacter xylinus	1306:1452	Additionally, both K. baliensis strains harbor identical gum-like clusters, which are related to the well characterized gum cluster coding for xanthan synthesis in Xanthomanas campestris and show highest similarity with gum-like heteropolysaccharide (HePS) clusters from other acetic acid bacteria such as Gluconacetobacter diazotrophicus and Komagataeibacter xylinus.
27716345	0	115	theme	biosynthesis	32:43	arg1	Dissection					0:9	Dissection	0:9	Dissection of exopolysaccharide biosynthesis in Kozakia baliensis.	0:65	Dissection of exopolysaccharide biosynthesis in Kozakia baliensis.
27716345	13	116	theme	present	2356:2362	arg1	study					2364:2368	the present study	2352:2368	the present study	2352:2368	Consequently, the present study provides the basis for establishment of K. baliensis strains as novel microbial cell factories for biotechnologically relevant, unique polysaccharides.
27716345	3	117	theme	baliensis	354:362	arg1	strains					364:370	two K. baliensis strains	347:370	two K. baliensis strains (DSM 14400, NBRC 16680) on sucrose-deficient media	347:421	Throughout cultivation of two K. baliensis strains (DSM 14400, NBRC 16680) on sucrose-deficient media, we found that both strains still produce high amounts of mucous, water-soluble substances from mannitol and glycerol as (main) carbon sources.
27716345	12	118	theme	genome	2163:2168	arg1	sequencing					2170:2179	genome sequencing	2163:2179	genome sequencing of K. baliensis DSM 14400 + NBRC 16680	2163:2218	Via genome sequencing of K. baliensis DSM 14400 + NBRC 16680 we got first insights into the biosynthesis of these novel HePS, which is related to xanthan and acetan biosynthesis.
27716345	0	119	theme	Kozakia	48:54	arg1	baliensis					56:64	Kozakia baliensis	48:64	Kozakia baliensis	48:64	Dissection of exopolysaccharide biosynthesis in Kozakia baliensis.
27716345	8	120	theme	xanthan	1229:1235	arg1	synthesis					1237:1245	xanthan synthesis	1229:1245	xanthan synthesis in Xanthomanas campestris	1229:1271	Additionally, both K. baliensis strains harbor identical gum-like clusters, which are related to the well characterized gum cluster coding for xanthan synthesis in Xanthomanas campestris and show highest similarity with gum-like heteropolysaccharide (HePS) clusters from other acetic acid bacteria such as Gluconacetobacter diazotrophicus and Komagataeibacter xylinus.
27716345	10	121	with	agreement	1882:1890	arg1	composition					1925:1935	the predicted sugar monomer composition	1897:1935	the predicted sugar monomer composition derived from in silico genome analysis of the respective gum-like clusters	1897:2010	The EPS secreted by K. baliensis are composed of glucose, galactose and mannose, respectively, which is in agreement with the predicted sugar monomer composition derived from in silico genome analysis of the respective gum-like clusters.
27716345	8	122	theme	other	1357:1361	arg1	xylinus					1446:1452	Komagataeibacter xylinus	1429:1452	Komagataeibacter xylinus	1429:1452	Additionally, both K. baliensis strains harbor identical gum-like clusters, which are related to the well characterized gum cluster coding for xanthan synthesis in Xanthomanas campestris and show highest similarity with gum-like heteropolysaccharide (HePS) clusters from other acetic acid bacteria such as Gluconacetobacter diazotrophicus and Komagataeibacter xylinus.
27716345	8	122	theme	other	1357:1361	arg1	bacteria					1375:1382	other acetic acid bacteria	1357:1382	other acetic acid bacteria such as Gluconacetobacter diazotrophicus and Komagataeibacter xylinus	1357:1452	Additionally, both K. baliensis strains harbor identical gum-like clusters, which are related to the well characterized gum cluster coding for xanthan synthesis in Xanthomanas campestris and show highest similarity with gum-like heteropolysaccharide (HePS) clusters from other acetic acid bacteria such as Gluconacetobacter diazotrophicus and Komagataeibacter xylinus.
27716345	8	122	theme	other	1357:1361	arg1	diazotrophicus					1410:1423	Gluconacetobacter diazotrophicus	1392:1423	Gluconacetobacter diazotrophicus	1392:1423	Additionally, both K. baliensis strains harbor identical gum-like clusters, which are related to the well characterized gum cluster coding for xanthan synthesis in Xanthomanas campestris and show highest similarity with gum-like heteropolysaccharide (HePS) clusters from other acetic acid bacteria such as Gluconacetobacter diazotrophicus and Komagataeibacter xylinus.
27716345	3	123	from	media	417:421	arg1	cultivation					332:342	cultivation	332:342	cultivation of two K. baliensis strains (DSM 14400, NBRC 16680) on sucrose-deficient media	332:421	Throughout cultivation of two K. baliensis strains (DSM 14400, NBRC 16680) on sucrose-deficient media, we found that both strains still produce high amounts of mucous, water-soluble substances from mannitol and glycerol as (main) carbon sources.
27716345	9	124	theme	gumD	1597:1600	arg1	gene					1602:1605	the gumD gene	1593:1605	the gumD gene	1593:1605	A mutant strain of K. baliensis NBRC 16680 lacking EPS production on sucrose-deficient media exhibited a transposon insertion in front of the gumD gene of its gum-like cluster in contrast to the wildtype strain, which indicated the essential role of gumD and of the associated gum genes for production of these new EPS.
27716345	13	125	theme	microbial	2440:2448	arg1	factories					2455:2463	novel microbial cell factories	2434:2463	novel microbial cell factories	2434:2463	Consequently, the present study provides the basis for establishment of K. baliensis strains as novel microbial cell factories for biotechnologically relevant, unique polysaccharides.
27716345	8	126	with	similarity	1290:1299	arg1	clusters					1343:1350	gum-like heteropolysaccharide (HePS) clusters	1306:1350	gum-like heteropolysaccharide (HePS) clusters from other acetic acid bacteria such as Gluconacetobacter diazotrophicus and Komagataeibacter xylinus	1306:1452	Additionally, both K. baliensis strains harbor identical gum-like clusters, which are related to the well characterized gum cluster coding for xanthan synthesis in Xanthomanas campestris and show highest similarity with gum-like heteropolysaccharide (HePS) clusters from other acetic acid bacteria such as Gluconacetobacter diazotrophicus and Komagataeibacter xylinus.
27716345	8	127	theme	acid	1370:1373	arg1	xylinus					1446:1452	Komagataeibacter xylinus	1429:1452	Komagataeibacter xylinus	1429:1452	Additionally, both K. baliensis strains harbor identical gum-like clusters, which are related to the well characterized gum cluster coding for xanthan synthesis in Xanthomanas campestris and show highest similarity with gum-like heteropolysaccharide (HePS) clusters from other acetic acid bacteria such as Gluconacetobacter diazotrophicus and Komagataeibacter xylinus.
27716345	8	127	theme	acid	1370:1373	arg1	bacteria					1375:1382	other acetic acid bacteria	1357:1382	other acetic acid bacteria such as Gluconacetobacter diazotrophicus and Komagataeibacter xylinus	1357:1452	Additionally, both K. baliensis strains harbor identical gum-like clusters, which are related to the well characterized gum cluster coding for xanthan synthesis in Xanthomanas campestris and show highest similarity with gum-like heteropolysaccharide (HePS) clusters from other acetic acid bacteria such as Gluconacetobacter diazotrophicus and Komagataeibacter xylinus.
27716345	8	127	theme	acid	1370:1373	arg1	diazotrophicus					1410:1423	Gluconacetobacter diazotrophicus	1392:1423	Gluconacetobacter diazotrophicus	1392:1423	Additionally, both K. baliensis strains harbor identical gum-like clusters, which are related to the well characterized gum cluster coding for xanthan synthesis in Xanthomanas campestris and show highest similarity with gum-like heteropolysaccharide (HePS) clusters from other acetic acid bacteria such as Gluconacetobacter diazotrophicus and Komagataeibacter xylinus.
27716345	12	128	theme	baliensis	2187:2195	arg1	14400 + NBRC					2201:2212	K. baliensis DSM 14400 + NBRC 16680	2184:2218	K. baliensis DSM 14400 + NBRC 16680	2184:2218	Via genome sequencing of K. baliensis DSM 14400 + NBRC 16680 we got first insights into the biosynthesis of these novel HePS, which is related to xanthan and acetan biosynthesis.
27716345	9	129	theme	gum-like	1614:1621	arg1	cluster					1623:1629	its gum-like cluster	1610:1629	its gum-like cluster	1610:1629	A mutant strain of K. baliensis NBRC 16680 lacking EPS production on sucrose-deficient media exhibited a transposon insertion in front of the gumD gene of its gum-like cluster in contrast to the wildtype strain, which indicated the essential role of gumD and of the associated gum genes for production of these new EPS.
27716345	10	130	theme	clusters	2003:2010	arg1	analysis					1967:1974	in silico genome analysis	1950:1974	in silico genome analysis of the respective gum-like clusters	1950:2010	The EPS secreted by K. baliensis are composed of glucose, galactose and mannose, respectively, which is in agreement with the predicted sugar monomer composition derived from in silico genome analysis of the respective gum-like clusters.
27716345	8	131	theme	Gluconacetobacter	1392:1408	arg1	diazotrophicus					1410:1423	Gluconacetobacter diazotrophicus	1392:1423	Gluconacetobacter diazotrophicus	1392:1423	Additionally, both K. baliensis strains harbor identical gum-like clusters, which are related to the well characterized gum cluster coding for xanthan synthesis in Xanthomanas campestris and show highest similarity with gum-like heteropolysaccharide (HePS) clusters from other acetic acid bacteria such as Gluconacetobacter diazotrophicus and Komagataeibacter xylinus.
27716345	9	132	theme	new	1766:1768	arg1	EPS					1770:1772	these new EPS	1760:1772	these new EPS	1760:1772	A mutant strain of K. baliensis NBRC 16680 lacking EPS production on sucrose-deficient media exhibited a transposon insertion in front of the gumD gene of its gum-like cluster in contrast to the wildtype strain, which indicated the essential role of gumD and of the associated gum genes for production of these new EPS.
24506235	14	0	theme	major	1222:1226	arg1	influences					1228:1237	major influences	1222:1237	major influences	1222:1237	Granule size, polymer proportion, and molecular weights had major influences on gelatinization and pasting properties of Great Northern bean starches.
24506235	2	1	dep	cultivars	165:173	arg1	Beryl-R					176:182	Beryl-R	176:182	Beryl-R	176:182	UNLABELLED The compositions and properties of 5 Great Northern bean cultivars (Beryl-R, Coyne, Gemini, Marquis, and Orion) were investigated.
24506235	2	1	dep	cultivars	165:173	arg1	Marquis					200:206	Marquis	200:206	Marquis	200:206	UNLABELLED The compositions and properties of 5 Great Northern bean cultivars (Beryl-R, Coyne, Gemini, Marquis, and Orion) were investigated.
24506235	2	1	dep	cultivars	165:173	arg1	Orion					213:217	Orion	213:217	Orion	213:217	UNLABELLED The compositions and properties of 5 Great Northern bean cultivars (Beryl-R, Coyne, Gemini, Marquis, and Orion) were investigated.
24506235	2	1	dep	cultivars	165:173	arg1	Gemini					192:197	Gemini	192:197	Gemini	192:197	UNLABELLED The compositions and properties of 5 Great Northern bean cultivars (Beryl-R, Coyne, Gemini, Marquis, and Orion) were investigated.
24506235	4	2	theme	granular	357:364	arg1	properties					403:412	granular, molecular, thermal, and rheological properties	357:412	granular, molecular, thermal, and rheological properties	357:412	Isolated, unmodified starches were characterized for granular, molecular, thermal, and rheological properties.
24506235	2	3	theme	Northern	151:158	arg1	cultivars					165:173	5 Great Northern bean cultivars	143:173	5 Great Northern bean cultivars (Beryl-R, Coyne, Gemini, Marquis, and Orion)	143:218	UNLABELLED The compositions and properties of 5 Great Northern bean cultivars (Beryl-R, Coyne, Gemini, Marquis, and Orion) were investigated.
24506235	14	4	contain	had	1218:1220	arg2	influences					1228:1237	major influences	1222:1237	major influences	1222:1237	Granule size, polymer proportion, and molecular weights had major influences on gelatinization and pasting properties of Great Northern bean starches.
24506235	14	4	contain	had	1218:1220	arg1	size					1170:1173	Granule size	1162:1173	Granule size	1162:1173	Granule size, polymer proportion, and molecular weights had major influences on gelatinization and pasting properties of Great Northern bean starches.
24506235	14	4	contain	had	1218:1220	arg1	proportion					1184:1193	polymer proportion	1176:1193	polymer proportion	1176:1193	Granule size, polymer proportion, and molecular weights had major influences on gelatinization and pasting properties of Great Northern bean starches.
24506235	14	4	contain	had	1218:1220	arg1	weights					1210:1216	molecular weights	1200:1216	molecular weights	1200:1216	Granule size, polymer proportion, and molecular weights had major influences on gelatinization and pasting properties of Great Northern bean starches.
24506235	18	5	theme	Northern	1761:1768	arg1	cultivars					1775:1783	5 Great Northern bean cultivars	1753:1783	5 Great Northern bean cultivars	1753:1783	This study investigated the properties and thermal behaviors of 5 Great Northern bean cultivars.
24506235	4	6	theme	molecular	367:375	arg1	properties					403:412	granular, molecular, thermal, and rheological properties	357:412	granular, molecular, thermal, and rheological properties	357:412	Isolated, unmodified starches were characterized for granular, molecular, thermal, and rheological properties.
24506235	13	7	theme	final	1143:1147	arg1	viscosities					1149:1159	final viscosities	1143:1159	final viscosities	1143:1159	In pasting profile analysis, Coyne had the lowest peak and final viscosities.
24506235	12	8	theme	gelatinization	1057:1070	arg1	enthalpies					1072:1081	low gelatinization enthalpies	1053:1081	low gelatinization enthalpies	1053:1081	Coyne and Gemini had low gelatinization enthalpies.
24506235	15	9	theme	varieties	1375:1383	arg1	one					1358:1360	one	1358:1360	one	1358:1360	PRACTICAL APPLICATION Great Northern bean is one of the major varieties of dry-edible beans produced worldwide.
24506235	15	9	theme	varieties	1375:1383	arg1	varieties					1375:1383	the major varieties	1365:1383	the major varieties of dry-edible beans produced worldwide	1365:1422	PRACTICAL APPLICATION Great Northern bean is one of the major varieties of dry-edible beans produced worldwide.
24506235	15	9	theme	varieties	1375:1383	arg1	beans					1399:1403	dry-edible beans	1388:1403	dry-edible beans produced worldwide	1388:1422	PRACTICAL APPLICATION Great Northern bean is one of the major varieties of dry-edible beans produced worldwide.
24506235	6	10	theme	%	554:554	arg1	%					563:563	the range 21.0% to 22.6%	540:563	the range 21.0% to 22.6%	540:563	Amylose contents were in the range 21.0% to 22.6%.
24506235	5	11	theme	similar	447:453	arg1	sizes					463:467	granule sizes	455:467	granule sizes	455:467	Smooth surfaces and essentially similar granule sizes and shapes were observed among all cultivars.
24506235	14	12	theme	Granule	1162:1168	arg1	size					1170:1173	Granule size	1162:1173	Granule size	1162:1173	Granule size, polymer proportion, and molecular weights had major influences on gelatinization and pasting properties of Great Northern bean starches.
24506235	6	13	theme	range	544:548	arg1	%					563:563	the range 21.0% to 22.6%	540:563	the range 21.0% to 22.6%	540:563	Amylose contents were in the range 21.0% to 22.6%.
24506235	2	14	theme	UNLABELLED	97:106	arg1	compositions					112:123	compositions	112:123	compositions	112:123	UNLABELLED The compositions and properties of 5 Great Northern bean cultivars (Beryl-R, Coyne, Gemini, Marquis, and Orion) were investigated.
24506235	19	15	theme	new	1790:1792	arg1	information					1794:1804	The new information	1786:1804	The new information reported in this article	1786:1829	The new information reported in this article, on starch properties, would pave ways to find new ingredient and product applications for Great Northern beans in food processing.
24506235	8	16	theme	X-ray	676:680	arg1	pattern					682:688	Typical C-type X-ray pattern	661:688	Typical C-type X-ray pattern	661:688	Typical C-type X-ray pattern was observed in all cultivars.
24506235	11	17	theme	Great	948:952	arg1	cultivars					968:976	The 5 Great Northern bean cultivars	942:976	The 5 Great Northern bean cultivars	942:976	The 5 Great Northern bean cultivars differed in their thermal and rheological properties.
24506235	6	18	dep	%	563:563	arg1	to					556:557	to	556:557	to	556:557	Amylose contents were in the range 21.0% to 22.6%.
24506235	4	19	theme	thermal	378:384	arg1	properties					403:412	granular, molecular, thermal, and rheological properties	357:412	granular, molecular, thermal, and rheological properties	357:412	Isolated, unmodified starches were characterized for granular, molecular, thermal, and rheological properties.
24506235	13	20	theme	lowest	1127:1132	arg1	peak					1134:1137	peak	1134:1137	peak	1134:1137	In pasting profile analysis, Coyne had the lowest peak and final viscosities.
24506235	3	21	theme	milling	287:293	arg1	process					295:301	a wet milling process	281:301	a wet milling process	281:301	Starch was isolated from each cultivar by a wet milling process.
24506235	13	22	theme	profile	1095:1101	arg1	analysis					1103:1110	pasting profile analysis	1087:1110	pasting profile analysis	1087:1110	In pasting profile analysis, Coyne had the lowest peak and final viscosities.
24506235	7	23	theme	molecular	590:598	arg1	weights					600:606	Amylose and amylopectin molecular weights	566:606	Amylose and amylopectin molecular weights	566:606	Amylose and amylopectin molecular weights were approximately 10(5) and 10(9) Da, respectively.
24506235	11	24	theme	bean	963:966	arg1	cultivars					968:976	The 5 Great Northern bean cultivars	942:976	The 5 Great Northern bean cultivars	942:976	The 5 Great Northern bean cultivars differed in their thermal and rheological properties.
24506235	16	25	theme	major	1439:1443	arg1	Starch					1425:1430	Starch	1425:1430	Starch	1425:1430	Starch is the major component in Great Northern beans, which accounts for approximately 40% of its composition.
24506235	16	25	theme	major	1439:1443	arg1	component					1445:1453	the major component	1435:1453	the major component	1435:1453	Starch is the major component in Great Northern beans, which accounts for approximately 40% of its composition.
24506235	15	26	theme	dry-edible	1388:1397	arg1	beans					1399:1403	dry-edible beans	1388:1403	dry-edible beans produced worldwide	1388:1422	PRACTICAL APPLICATION Great Northern bean is one of the major varieties of dry-edible beans produced worldwide.
24506235	18	27	theme	thermal	1732:1738	arg1	behaviors					1740:1748	thermal behaviors	1732:1748	thermal behaviors	1732:1748	This study investigated the properties and thermal behaviors of 5 Great Northern bean cultivars.
24506235	14	28	theme	bean	1298:1301	arg1	starches					1303:1310	Great Northern bean starches	1283:1310	Great Northern bean starches	1283:1310	Granule size, polymer proportion, and molecular weights had major influences on gelatinization and pasting properties of Great Northern bean starches.
24506235	1	29	theme	Northern	75:82	arg1	starch					89:94	Great Northern bean starch	69:94	Great Northern bean starch.	69:95	Great Northern bean starch.
24506235	17	30	theme	most	1546:1549	arg1	starches					1558:1565	most legume starches	1546:1565	most legume starches	1546:1565	Although most legume starches have been studied in detail, physicochemical and functional properties of Great Northern bean starch are largely unknown.
24506235	14	31	theme	Great	1283:1287	arg1	starches					1303:1310	Great Northern bean starches	1283:1310	Great Northern bean starches	1283:1310	Granule size, polymer proportion, and molecular weights had major influences on gelatinization and pasting properties of Great Northern bean starches.
24506235	9	32	theme	percentage	778:787	arg1	crystallinities					798:812	percentage relative crystallinities	778:812	percentage relative crystallinities	778:812	Significant differences were observed among cultivars in percentage relative crystallinities, which were in the range 18.2% to 23.8%.
24506235	16	33	theme	Northern	1464:1471	arg1	beans					1473:1477	Great Northern beans	1458:1477	Great Northern beans	1458:1477	Starch is the major component in Great Northern beans, which accounts for approximately 40% of its composition.
24506235	0	34	theme	Physicochemical	0:14	arg1	properties					28:37	Physicochemical and thermal properties	0:37	Physicochemical and thermal properties of Phaseolus vulgaris	0:59	Physicochemical and thermal properties of Phaseolus vulgaris L. var.
24506235	10	35	theme	relative	859:866	arg1	independent					886:896	independent	886:896	independent	886:896	The relative crystallinity was independent of amylose proportion and molecular weight.
24506235	10	35	theme	relative	859:866	arg1	crystallinity					868:880	The relative crystallinity	855:880	The relative crystallinity	855:880	The relative crystallinity was independent of amylose proportion and molecular weight.
24506235	17	36	theme	starch	1661:1666	arg1	properties					1627:1636	physicochemical and functional properties	1596:1636	physicochemical and functional properties of Great Northern bean starch	1596:1666	Although most legume starches have been studied in detail, physicochemical and functional properties of Great Northern bean starch are largely unknown.
24506235	10	37	theme	amylose	901:907	arg1	proportion					909:918	amylose proportion	901:918	amylose proportion	901:918	The relative crystallinity was independent of amylose proportion and molecular weight.
24506235	0	38	theme	thermal	20:26	arg1	properties					28:37	Physicochemical and thermal properties	0:37	Physicochemical and thermal properties of Phaseolus vulgaris	0:59	Physicochemical and thermal properties of Phaseolus vulgaris L. var.
24506235	9	39	dep	%	852:852	arg1	to					845:846	to	845:846	to	845:846	Significant differences were observed among cultivars in percentage relative crystallinities, which were in the range 18.2% to 23.8%.
24506235	0	40	dep	var	64:66	arg1	properties					28:37	Physicochemical and thermal properties	0:37	Physicochemical and thermal properties of Phaseolus vulgaris	0:59	Physicochemical and thermal properties of Phaseolus vulgaris L. var.
24506235	4	41	theme	Isolated	304:311	arg1	starches					325:332	Isolated, unmodified starches	304:332	Isolated, unmodified starches	304:332	Isolated, unmodified starches were characterized for granular, molecular, thermal, and rheological properties.
24506235	2	42	dep	compositions	112:123	arg1	The					108:110	The	108:110	The	108:110	UNLABELLED The compositions and properties of 5 Great Northern bean cultivars (Beryl-R, Coyne, Gemini, Marquis, and Orion) were investigated.
24506235	2	43	theme	bean	160:163	arg1	cultivars					165:173	5 Great Northern bean cultivars	143:173	5 Great Northern bean cultivars (Beryl-R, Coyne, Gemini, Marquis, and Orion)	143:218	UNLABELLED The compositions and properties of 5 Great Northern bean cultivars (Beryl-R, Coyne, Gemini, Marquis, and Orion) were investigated.
24506235	15	44	theme	Great	1335:1339	arg1	bean					1350:1353	PRACTICAL APPLICATION Great Northern bean	1313:1353	PRACTICAL APPLICATION Great Northern bean	1313:1353	PRACTICAL APPLICATION Great Northern bean is one of the major varieties of dry-edible beans produced worldwide.
24506235	0	45	theme	vulgaris	52:59	arg1	properties					28:37	Physicochemical and thermal properties	0:37	Physicochemical and thermal properties of Phaseolus vulgaris	0:59	Physicochemical and thermal properties of Phaseolus vulgaris L. var.
24506235	19	46	from	beans	1937:1941	arg1	processing					1951:1960	food processing	1946:1960	food processing	1946:1960	The new information reported in this article, on starch properties, would pave ways to find new ingredient and product applications for Great Northern beans in food processing.
24506235	19	47	theme	product	1897:1903	arg1	applications					1905:1916	new ingredient and product applications	1878:1916	new ingredient and product applications	1878:1916	The new information reported in this article, on starch properties, would pave ways to find new ingredient and product applications for Great Northern beans in food processing.
24506235	9	48	theme	range	833:837	arg1	%					852:852	the range 18.2% to 23.8%	829:852	the range 18.2% to 23.8%	829:852	Significant differences were observed among cultivars in percentage relative crystallinities, which were in the range 18.2% to 23.8%.
24506235	15	49	theme	PRACTICAL	1313:1321	arg1	APPLICATION					1323:1333	PRACTICAL APPLICATION	1313:1333	PRACTICAL APPLICATION Great Northern bean	1313:1353	PRACTICAL APPLICATION Great Northern bean is one of the major varieties of dry-edible beans produced worldwide.
24506235	10	50	theme	molecular	924:932	arg1	weight					934:939	molecular weight	924:939	molecular weight	924:939	The relative crystallinity was independent of amylose proportion and molecular weight.
24506235	19	51	theme	ingredient	1882:1891	arg1	applications					1905:1916	new ingredient and product applications	1878:1916	new ingredient and product applications	1878:1916	The new information reported in this article, on starch properties, would pave ways to find new ingredient and product applications for Great Northern beans in food processing.
24506235	9	52	theme	%	843:843	arg1	%					852:852	the range 18.2% to 23.8%	829:852	the range 18.2% to 23.8%	829:852	Significant differences were observed among cultivars in percentage relative crystallinities, which were in the range 18.2% to 23.8%.
24506235	10	53	theme	proportion	909:918	arg1	independent					886:896	independent	886:896	independent	886:896	The relative crystallinity was independent of amylose proportion and molecular weight.
24506235	10	53	theme	proportion	909:918	arg1	crystallinity					868:880	The relative crystallinity	855:880	The relative crystallinity	855:880	The relative crystallinity was independent of amylose proportion and molecular weight.
24506235	19	54	theme	Northern	1928:1935	arg1	beans					1937:1941	Great Northern beans	1922:1941	Great Northern beans in food processing	1922:1960	The new information reported in this article, on starch properties, would pave ways to find new ingredient and product applications for Great Northern beans in food processing.
24506235	6	55	theme	Amylose	515:521	arg1	contents					523:530	Amylose contents	515:530	Amylose contents	515:530	Amylose contents were in the range 21.0% to 22.6%.
24506235	17	56	theme	Great	1641:1645	arg1	starch					1661:1666	Great Northern bean starch	1641:1666	Great Northern bean starch	1641:1666	Although most legume starches have been studied in detail, physicochemical and functional properties of Great Northern bean starch are largely unknown.
24506235	14	57	theme	molecular	1200:1208	arg1	weights					1210:1216	molecular weights	1200:1216	molecular weights	1200:1216	Granule size, polymer proportion, and molecular weights had major influences on gelatinization and pasting properties of Great Northern bean starches.
24506235	17	58	theme	bean	1656:1659	arg1	starch					1661:1666	Great Northern bean starch	1641:1666	Great Northern bean starch	1641:1666	Although most legume starches have been studied in detail, physicochemical and functional properties of Great Northern bean starch are largely unknown.
24506235	2	59	theme	Great	145:149	arg1	cultivars					165:173	5 Great Northern bean cultivars	143:173	5 Great Northern bean cultivars (Beryl-R, Coyne, Gemini, Marquis, and Orion)	143:218	UNLABELLED The compositions and properties of 5 Great Northern bean cultivars (Beryl-R, Coyne, Gemini, Marquis, and Orion) were investigated.
24506235	18	60	theme	Great	1755:1759	arg1	cultivars					1775:1783	5 Great Northern bean cultivars	1753:1783	5 Great Northern bean cultivars	1753:1783	This study investigated the properties and thermal behaviors of 5 Great Northern bean cultivars.
24506235	11	61	theme	thermal	996:1002	arg1	properties					1020:1029	their thermal and rheological properties	990:1029	their thermal and rheological properties	990:1029	The 5 Great Northern bean cultivars differed in their thermal and rheological properties.
24506235	14	62	theme	polymer	1176:1182	arg1	proportion					1184:1193	polymer proportion	1176:1193	polymer proportion	1176:1193	Granule size, polymer proportion, and molecular weights had major influences on gelatinization and pasting properties of Great Northern bean starches.
24506235	18	63	dep	properties	1717:1726	arg1	the					1713:1715	the	1713:1715	the	1713:1715	This study investigated the properties and thermal behaviors of 5 Great Northern bean cultivars.
24506235	15	64	theme	major	1369:1373	arg1	varieties					1375:1383	the major varieties	1365:1383	the major varieties of dry-edible beans produced worldwide	1365:1422	PRACTICAL APPLICATION Great Northern bean is one of the major varieties of dry-edible beans produced worldwide.
24506235	15	64	theme	major	1369:1373	arg1	beans					1399:1403	dry-edible beans	1388:1403	dry-edible beans produced worldwide	1388:1422	PRACTICAL APPLICATION Great Northern bean is one of the major varieties of dry-edible beans produced worldwide.
24506235	5	65	theme	granule	455:461	arg1	sizes					463:467	granule sizes	455:467	granule sizes	455:467	Smooth surfaces and essentially similar granule sizes and shapes were observed among all cultivars.
24506235	11	66	theme	rheological	1008:1018	arg1	properties					1020:1029	their thermal and rheological properties	990:1029	their thermal and rheological properties	990:1029	The 5 Great Northern bean cultivars differed in their thermal and rheological properties.
24506235	8	67	theme	C-type	669:674	arg1	pattern					682:688	Typical C-type X-ray pattern	661:688	Typical C-type X-ray pattern	661:688	Typical C-type X-ray pattern was observed in all cultivars.
24506235	4	68	theme	rheological	391:401	arg1	properties					403:412	granular, molecular, thermal, and rheological properties	357:412	granular, molecular, thermal, and rheological properties	357:412	Isolated, unmodified starches were characterized for granular, molecular, thermal, and rheological properties.
24506235	13	69	theme	pasting	1087:1093	arg1	analysis					1103:1110	pasting profile analysis	1087:1110	pasting profile analysis	1087:1110	In pasting profile analysis, Coyne had the lowest peak and final viscosities.
24506235	16	70	from	component	1445:1453	arg1	beans					1473:1477	Great Northern beans	1458:1477	Great Northern beans	1458:1477	Starch is the major component in Great Northern beans, which accounts for approximately 40% of its composition.
24506235	19	71	theme	food	1946:1949	arg1	processing					1951:1960	food processing	1946:1960	food processing	1946:1960	The new information reported in this article, on starch properties, would pave ways to find new ingredient and product applications for Great Northern beans in food processing.
24506235	17	72	theme	physicochemical	1596:1610	arg1	properties					1627:1636	physicochemical and functional properties	1596:1636	physicochemical and functional properties of Great Northern bean starch	1596:1666	Although most legume starches have been studied in detail, physicochemical and functional properties of Great Northern bean starch are largely unknown.
24506235	13	73	contain	had	1119:1121	arg2	peak					1134:1137	peak	1134:1137	peak	1134:1137	In pasting profile analysis, Coyne had the lowest peak and final viscosities.
24506235	13	73	contain	had	1119:1121	arg2	viscosities					1149:1159	final viscosities	1143:1159	final viscosities	1143:1159	In pasting profile analysis, Coyne had the lowest peak and final viscosities.
24506235	13	73	contain	had	1119:1121	arg1	Coyne					1113:1117	Coyne	1113:1117	Coyne	1113:1117	In pasting profile analysis, Coyne had the lowest peak and final viscosities.
24506235	9	74	theme	Significant	721:731	arg1	differences					733:743	Significant differences	721:743	Significant differences	721:743	Significant differences were observed among cultivars in percentage relative crystallinities, which were in the range 18.2% to 23.8%.
24506235	16	75	theme	composition	1524:1534	arg1	%					1515:1515	approximately 40%	1499:1515	approximately 40% of its composition	1499:1534	Starch is the major component in Great Northern beans, which accounts for approximately 40% of its composition.
24506235	16	75	theme	composition	1524:1534	arg1	composition					1524:1534	its composition	1520:1534	its composition	1520:1534	Starch is the major component in Great Northern beans, which accounts for approximately 40% of its composition.
24506235	11	76	theme	Northern	954:961	arg1	cultivars					968:976	The 5 Great Northern bean cultivars	942:976	The 5 Great Northern bean cultivars	942:976	The 5 Great Northern bean cultivars differed in their thermal and rheological properties.
24506235	17	77	theme	functional	1616:1625	arg1	properties					1627:1636	physicochemical and functional properties	1596:1636	physicochemical and functional properties of Great Northern bean starch	1596:1666	Although most legume starches have been studied in detail, physicochemical and functional properties of Great Northern bean starch are largely unknown.
24506235	7	78	dep	10	627:628	arg1	Da					643:644	Da	643:644	Da	643:644	Amylose and amylopectin molecular weights were approximately 10(5) and 10(9) Da, respectively.
24506235	3	79	theme	wet	283:285	arg1	process					295:301	a wet milling process	281:301	a wet milling process	281:301	Starch was isolated from each cultivar by a wet milling process.
24506235	16	80	theme	Great	1458:1462	arg1	beans					1473:1477	Great Northern beans	1458:1477	Great Northern beans	1458:1477	Starch is the major component in Great Northern beans, which accounts for approximately 40% of its composition.
24506235	14	81	theme	starches	1303:1310	arg1	properties					1269:1278	gelatinization and pasting properties	1242:1278	gelatinization and pasting properties of Great Northern bean starches	1242:1310	Granule size, polymer proportion, and molecular weights had major influences on gelatinization and pasting properties of Great Northern bean starches.
24506235	8	82	located	observed	694:701	arg1	cultivars					710:718	all cultivars	706:718	all cultivars	706:718	Typical C-type X-ray pattern was observed in all cultivars.
24506235	8	82	located	observed	694:701	arg2	pattern					682:688	Typical C-type X-ray pattern	661:688	Typical C-type X-ray pattern	661:688	Typical C-type X-ray pattern was observed in all cultivars.
24506235	18	83	theme	cultivars	1775:1783	arg1	properties					1717:1726	properties	1717:1726	properties	1717:1726	This study investigated the properties and thermal behaviors of 5 Great Northern bean cultivars.
24506235	18	83	theme	cultivars	1775:1783	arg1	behaviors					1740:1748	thermal behaviors	1732:1748	thermal behaviors	1732:1748	This study investigated the properties and thermal behaviors of 5 Great Northern bean cultivars.
24506235	15	84	theme	beans	1399:1403	arg1	varieties					1375:1383	the major varieties	1365:1383	the major varieties of dry-edible beans produced worldwide	1365:1422	PRACTICAL APPLICATION Great Northern bean is one of the major varieties of dry-edible beans produced worldwide.
24506235	15	84	theme	beans	1399:1403	arg1	beans					1399:1403	dry-edible beans	1388:1403	dry-edible beans produced worldwide	1388:1422	PRACTICAL APPLICATION Great Northern bean is one of the major varieties of dry-edible beans produced worldwide.
24506235	7	85	theme	amylopectin	578:588	arg1	weights					600:606	Amylose and amylopectin molecular weights	566:606	Amylose and amylopectin molecular weights	566:606	Amylose and amylopectin molecular weights were approximately 10(5) and 10(9) Da, respectively.
24506235	1	86	theme	Great	69:73	arg1	starch					89:94	Great Northern bean starch	69:94	Great Northern bean starch.	69:95	Great Northern bean starch.
24506235	14	87	theme	Northern	1289:1296	arg1	starches					1303:1310	Great Northern bean starches	1283:1310	Great Northern bean starches	1283:1310	Granule size, polymer proportion, and molecular weights had major influences on gelatinization and pasting properties of Great Northern bean starches.
24506235	17	88	theme	Northern	1647:1654	arg1	starch					1661:1666	Great Northern bean starch	1641:1666	Great Northern bean starch	1641:1666	Although most legume starches have been studied in detail, physicochemical and functional properties of Great Northern bean starch are largely unknown.
24506235	7	89	theme	Amylose	566:572	arg1	weights					600:606	Amylose and amylopectin molecular weights	566:606	Amylose and amylopectin molecular weights	566:606	Amylose and amylopectin molecular weights were approximately 10(5) and 10(9) Da, respectively.
24506235	1	90	theme	bean	84:87	arg1	starch					89:94	Great Northern bean starch	69:94	Great Northern bean starch.	69:95	Great Northern bean starch.
24506235	17	91	theme	legume	1551:1556	arg1	starches					1558:1565	most legume starches	1546:1565	most legume starches	1546:1565	Although most legume starches have been studied in detail, physicochemical and functional properties of Great Northern bean starch are largely unknown.
24506235	19	92	theme	starch	1835:1840	arg1	properties					1842:1851	starch properties	1835:1851	starch properties	1835:1851	The new information reported in this article, on starch properties, would pave ways to find new ingredient and product applications for Great Northern beans in food processing.
24506235	9	93	theme	relative	789:796	arg1	crystallinities					798:812	percentage relative crystallinities	778:812	percentage relative crystallinities	778:812	Significant differences were observed among cultivars in percentage relative crystallinities, which were in the range 18.2% to 23.8%.
24506235	14	94	theme	pasting	1261:1267	arg1	properties					1269:1278	gelatinization and pasting properties	1242:1278	gelatinization and pasting properties of Great Northern bean starches	1242:1310	Granule size, polymer proportion, and molecular weights had major influences on gelatinization and pasting properties of Great Northern bean starches.
24506235	9	95	from	cultivars	765:773	arg1	crystallinities					798:812	percentage relative crystallinities	778:812	percentage relative crystallinities	778:812	Significant differences were observed among cultivars in percentage relative crystallinities, which were in the range 18.2% to 23.8%.
24506235	18	96	theme	bean	1770:1773	arg1	cultivars					1775:1783	5 Great Northern bean cultivars	1753:1783	5 Great Northern bean cultivars	1753:1783	This study investigated the properties and thermal behaviors of 5 Great Northern bean cultivars.
24506235	4	97	dep	Isolated	304:311	arg1	unmodified					314:323	unmodified	314:323	unmodified	314:323	Isolated, unmodified starches were characterized for granular, molecular, thermal, and rheological properties.
24506235	0	98	theme	Phaseolus	42:50	arg1	vulgaris					52:59	Phaseolus vulgaris	42:59	Phaseolus vulgaris	42:59	Physicochemical and thermal properties of Phaseolus vulgaris L. var.
24506235	12	99	contain	had	1049:1051	arg2	enthalpies					1072:1081	low gelatinization enthalpies	1053:1081	low gelatinization enthalpies	1053:1081	Coyne and Gemini had low gelatinization enthalpies.
24506235	12	99	contain	had	1049:1051	arg1	Gemini					1042:1047	Gemini	1042:1047	Gemini	1042:1047	Coyne and Gemini had low gelatinization enthalpies.
24506235	12	99	contain	had	1049:1051	arg1	Coyne					1032:1036	Coyne	1032:1036	Coyne	1032:1036	Coyne and Gemini had low gelatinization enthalpies.
24506235	14	100	theme	gelatinization	1242:1255	arg1	properties					1269:1278	gelatinization and pasting properties	1242:1278	gelatinization and pasting properties of Great Northern bean starches	1242:1310	Granule size, polymer proportion, and molecular weights had major influences on gelatinization and pasting properties of Great Northern bean starches.
24506235	3	101	attach	isolated	250:257	arg1	cultivar					269:276	each cultivar	264:276	each cultivar	264:276	Starch was isolated from each cultivar by a wet milling process.
24506235	3	101	attach	isolated	250:257	arg2	Starch					239:244	Starch	239:244	Starch	239:244	Starch was isolated from each cultivar by a wet milling process.
24506235	2	102	theme	cultivars	165:173	arg1	properties					129:138	properties	129:138	properties	129:138	UNLABELLED The compositions and properties of 5 Great Northern bean cultivars (Beryl-R, Coyne, Gemini, Marquis, and Orion) were investigated.
24506235	2	102	theme	cultivars	165:173	arg1	compositions					112:123	compositions	112:123	compositions	112:123	UNLABELLED The compositions and properties of 5 Great Northern bean cultivars (Beryl-R, Coyne, Gemini, Marquis, and Orion) were investigated.
24506235	12	103	theme	low	1053:1055	arg1	enthalpies					1072:1081	low gelatinization enthalpies	1053:1081	low gelatinization enthalpies	1053:1081	Coyne and Gemini had low gelatinization enthalpies.
24506235	8	104	theme	Typical	661:667	arg1	pattern					682:688	Typical C-type X-ray pattern	661:688	Typical C-type X-ray pattern	661:688	Typical C-type X-ray pattern was observed in all cultivars.
24506235	15	105	theme	Northern	1341:1348	arg1	bean					1350:1353	PRACTICAL APPLICATION Great Northern bean	1313:1353	PRACTICAL APPLICATION Great Northern bean	1313:1353	PRACTICAL APPLICATION Great Northern bean is one of the major varieties of dry-edible beans produced worldwide.
24506235	10	106	theme	weight	934:939	arg1	independent					886:896	independent	886:896	independent	886:896	The relative crystallinity was independent of amylose proportion and molecular weight.
24506235	10	106	theme	weight	934:939	arg1	crystallinity					868:880	The relative crystallinity	855:880	The relative crystallinity	855:880	The relative crystallinity was independent of amylose proportion and molecular weight.
24506235	19	107	theme	new	1878:1880	arg1	applications					1905:1916	new ingredient and product applications	1878:1916	new ingredient and product applications	1878:1916	The new information reported in this article, on starch properties, would pave ways to find new ingredient and product applications for Great Northern beans in food processing.
24506235	15	108	theme	APPLICATION	1323:1333	arg1	bean					1350:1353	PRACTICAL APPLICATION Great Northern bean	1313:1353	PRACTICAL APPLICATION Great Northern bean	1313:1353	PRACTICAL APPLICATION Great Northern bean is one of the major varieties of dry-edible beans produced worldwide.
24506235	5	109	theme	Smooth	415:420	arg1	surfaces					422:429	Smooth surfaces	415:429	Smooth surfaces	415:429	Smooth surfaces and essentially similar granule sizes and shapes were observed among all cultivars.
24506235	19	110	theme	Great	1922:1926	arg1	beans					1937:1941	Great Northern beans	1922:1941	Great Northern beans in food processing	1922:1960	The new information reported in this article, on starch properties, would pave ways to find new ingredient and product applications for Great Northern beans in food processing.
25828510	4	0	theme	global	551:556	arg1	results					558:564	The global results	547:564	The global results	547:564	The global results allowed an evident separation between both varieties and the water treatments.
25828510	5	1	from	CW	847:848	arg1	TR					853:854	TR	853:854	TR	853:854	WS resulted in increased anthocyanin contents in both varieties, reduced amounts in cellulose and lignin at maturation, but an increase in arabinose-containing polysaccharides more tightly bound to the CW in TR.
25828510	0	2	from	Expression	76:85	arg1	Varieties					132:140	Two Vitis vinifera L. Varieties	110:140	Two Vitis vinifera L. Varieties	110:140	Relating Water Deficiency to Berry Texture, Skin Cell Wall Composition, and Expression of Remodeling Genes in Two Vitis vinifera L. Varieties.
25828510	5	3	from	maturation	753:762	arg1	lignin					743:748	lignin	743:748	lignin	743:748	WS resulted in increased anthocyanin contents in both varieties, reduced amounts in cellulose and lignin at maturation, but an increase in arabinose-containing polysaccharides more tightly bound to the CW in TR.
25828510	5	3	from	maturation	753:762	arg1	cellulose					729:737	cellulose	729:737	cellulose	729:737	WS resulted in increased anthocyanin contents in both varieties, reduced amounts in cellulose and lignin at maturation, but an increase in arabinose-containing polysaccharides more tightly bound to the CW in TR.
25828510	5	3	from	maturation	753:762	arg1	amounts					718:724	reduced amounts	710:724	reduced amounts in cellulose and lignin at maturation	710:762	WS resulted in increased anthocyanin contents in both varieties, reduced amounts in cellulose and lignin at maturation, but an increase in arabinose-containing polysaccharides more tightly bound to the CW in TR.
25828510	7	4	from	alteration	1059:1068	arg1	structure					1080:1088	pectin structure	1073:1088	pectin structure	1073:1088	The results support the assumption that WS affects grape berries by stiffening the CW through alteration in pectin structure, supporting its involvement in responses to environmental conditions.
25828510	3	5	theme	present	300:306	arg1	work					308:311	the present work	296:311	the present work	296:311	In the present work, berry texture, skin CW composition, and expression of remodeling genes were investigated in two V. vinifera varieties, Touriga Nacional (TN) and Trincadeira (TR), under two water regimes, Full Irrigation (FI) and No Irrigation (NI).
25828510	5	6	from	contents	682:689	arg1	varieties					699:707	both varieties	694:707	both varieties	694:707	WS resulted in increased anthocyanin contents in both varieties, reduced amounts in cellulose and lignin at maturation, but an increase in arabinose-containing polysaccharides more tightly bound to the CW in TR.
25828510	7	7	theme	pectin	1073:1078	arg1	structure					1080:1088	pectin structure	1073:1088	pectin structure	1073:1088	The results support the assumption that WS affects grape berries by stiffening the CW through alteration in pectin structure, supporting its involvement in responses to environmental conditions.
25828510	0	8	theme	Remodeling	90:99	arg1	Genes					101:105	Remodeling Genes	90:105	Remodeling Genes	90:105	Relating Water Deficiency to Berry Texture, Skin Cell Wall Composition, and Expression of Remodeling Genes in Two Vitis vinifera L. Varieties.
25828510	5	9	theme	increased	660:668	arg1	contents					682:689	increased anthocyanin contents	660:689	increased anthocyanin contents in both varieties	660:707	WS resulted in increased anthocyanin contents in both varieties, reduced amounts in cellulose and lignin at maturation, but an increase in arabinose-containing polysaccharides more tightly bound to the CW in TR.
25828510	0	10	from	Texture	35:41	arg1	Varieties					132:140	Two Vitis vinifera L. Varieties	110:140	Two Vitis vinifera L. Varieties	110:140	Relating Water Deficiency to Berry Texture, Skin Cell Wall Composition, and Expression of Remodeling Genes in Two Vitis vinifera L. Varieties.
25828510	5	11	from	increase	772:779	arg1	polysaccharides					805:819	arabinose-containing polysaccharides	784:819	arabinose-containing polysaccharides more tightly bound to the CW in TR	784:854	WS resulted in increased anthocyanin contents in both varieties, reduced amounts in cellulose and lignin at maturation, but an increase in arabinose-containing polysaccharides more tightly bound to the CW in TR.
25828510	4	12	theme	water	627:631	arg1	treatments					633:642	the water treatments	623:642	the water treatments	623:642	The global results allowed an evident separation between both varieties and the water treatments.
25828510	1	13	theme	cell	147:150	arg1	wall					152:155	The cell wall	143:155	The cell wall (CW)	143:160	The cell wall (CW) is a dynamic structure that responds to stress.
25828510	1	13	theme	cell	147:150	arg1	structure					175:183	a dynamic structure	165:183	a dynamic structure that responds to stress	165:207	The cell wall (CW) is a dynamic structure that responds to stress.
25828510	1	13	theme	cell	147:150	arg1	CW					158:159	CW	158:159	CW	158:159	The cell wall (CW) is a dynamic structure that responds to stress.
25828510	0	14	theme	Water	9:13	arg1	Deficiency					15:24	Water Deficiency	9:24	Water Deficiency	9:24	Relating Water Deficiency to Berry Texture, Skin Cell Wall Composition, and Expression of Remodeling Genes in Two Vitis vinifera L. Varieties.
25828510	6	15	dep	WS	872:873	arg1	response					860:867	response	860:867	response	860:867	In response to WS, the majority of the CW related genes were down-regulated in a variety dependent pattern.
25828510	7	16	from	involvement	1106:1116	arg1	responses					1121:1129	responses	1121:1129	responses to environmental conditions	1121:1157	The results support the assumption that WS affects grape berries by stiffening the CW through alteration in pectin structure, supporting its involvement in responses to environmental conditions.
25828510	5	17	theme	arabinose-containing	784:803	arg1	polysaccharides					805:819	arabinose-containing polysaccharides	784:819	arabinose-containing polysaccharides more tightly bound to the CW in TR	784:854	WS resulted in increased anthocyanin contents in both varieties, reduced amounts in cellulose and lignin at maturation, but an increase in arabinose-containing polysaccharides more tightly bound to the CW in TR.
25828510	6	18	theme	related	899:905	arg1	genes					907:911	the CW related genes	892:911	the CW related genes	892:911	In response to WS, the majority of the CW related genes were down-regulated in a variety dependent pattern.
25828510	5	19	theme	anthocyanin	670:680	arg1	contents					682:689	increased anthocyanin contents	660:689	increased anthocyanin contents in both varieties	660:707	WS resulted in increased anthocyanin contents in both varieties, reduced amounts in cellulose and lignin at maturation, but an increase in arabinose-containing polysaccharides more tightly bound to the CW in TR.
25828510	2	20	theme	berry	248:252	arg1	composition					254:264	grapevine berry composition	238:264	grapevine berry composition	238:264	Water shortage (WS) impacts grapevine berry composition and its sensorial quality.
25828510	2	21	theme	grapevine	238:246	arg1	composition					254:264	grapevine berry composition	238:264	grapevine berry composition	238:264	Water shortage (WS) impacts grapevine berry composition and its sensorial quality.
25828510	7	22	theme	environmental	1134:1146	arg1	conditions					1148:1157	environmental conditions	1134:1157	environmental conditions	1134:1157	The results support the assumption that WS affects grape berries by stiffening the CW through alteration in pectin structure, supporting its involvement in responses to environmental conditions.
25828510	0	23	theme	Genes	101:105	arg1	Composition					59:69	Skin Cell Wall Composition	44:69	Skin Cell Wall Composition	44:69	Relating Water Deficiency to Berry Texture, Skin Cell Wall Composition, and Expression of Remodeling Genes in Two Vitis vinifera L. Varieties.
25828510	0	23	theme	Genes	101:105	arg1	Texture					35:41	Berry Texture	29:41	Berry Texture	29:41	Relating Water Deficiency to Berry Texture, Skin Cell Wall Composition, and Expression of Remodeling Genes in Two Vitis vinifera L. Varieties.
25828510	0	23	theme	Genes	101:105	arg1	Expression					76:85	Expression	76:85	Expression of Remodeling Genes in Two Vitis vinifera L. Varieties	76:140	Relating Water Deficiency to Berry Texture, Skin Cell Wall Composition, and Expression of Remodeling Genes in Two Vitis vinifera L. Varieties.
25828510	3	24	theme	CW	334:335	arg1	composition					337:347	skin CW composition	329:347	skin CW composition	329:347	In the present work, berry texture, skin CW composition, and expression of remodeling genes were investigated in two V. vinifera varieties, Touriga Nacional (TN) and Trincadeira (TR), under two water regimes, Full Irrigation (FI) and No Irrigation (NI).
25828510	3	25	theme	vinifera	413:420	arg1	Trincadeira					459:469	Trincadeira	459:469	Trincadeira	459:469	In the present work, berry texture, skin CW composition, and expression of remodeling genes were investigated in two V. vinifera varieties, Touriga Nacional (TN) and Trincadeira (TR), under two water regimes, Full Irrigation (FI) and No Irrigation (NI).
25828510	3	25	theme	vinifera	413:420	arg1	varieties					422:430	two V. vinifera varieties	406:430	two V. vinifera varieties	406:430	In the present work, berry texture, skin CW composition, and expression of remodeling genes were investigated in two V. vinifera varieties, Touriga Nacional (TN) and Trincadeira (TR), under two water regimes, Full Irrigation (FI) and No Irrigation (NI).
25828510	3	25	theme	vinifera	413:420	arg1	Nacional					441:448	Nacional	441:448	Nacional	441:448	In the present work, berry texture, skin CW composition, and expression of remodeling genes were investigated in two V. vinifera varieties, Touriga Nacional (TN) and Trincadeira (TR), under two water regimes, Full Irrigation (FI) and No Irrigation (NI).
25828510	0	26	theme	Vitis	114:118	arg1	vinifera					120:127	Vitis vinifera	114:127	Two Vitis vinifera L. Varieties	110:140	Relating Water Deficiency to Berry Texture, Skin Cell Wall Composition, and Expression of Remodeling Genes in Two Vitis vinifera L. Varieties.
25828510	0	27	from	Composition	59:69	arg1	Varieties					132:140	Two Vitis vinifera L. Varieties	110:140	Two Vitis vinifera L. Varieties	110:140	Relating Water Deficiency to Berry Texture, Skin Cell Wall Composition, and Expression of Remodeling Genes in Two Vitis vinifera L. Varieties.
25828510	3	28	theme	remodeling	368:377	arg1	genes					379:383	remodeling genes	368:383	remodeling genes	368:383	In the present work, berry texture, skin CW composition, and expression of remodeling genes were investigated in two V. vinifera varieties, Touriga Nacional (TN) and Trincadeira (TR), under two water regimes, Full Irrigation (FI) and No Irrigation (NI).
25828510	0	29	theme	Berry	29:33	arg1	Texture					35:41	Berry Texture	29:41	Berry Texture	29:41	Relating Water Deficiency to Berry Texture, Skin Cell Wall Composition, and Expression of Remodeling Genes in Two Vitis vinifera L. Varieties.
25828510	3	30	theme	water	487:491	arg1	Irrigation					530:539	No Irrigation	527:539	No Irrigation (NI)	527:544	In the present work, berry texture, skin CW composition, and expression of remodeling genes were investigated in two V. vinifera varieties, Touriga Nacional (TN) and Trincadeira (TR), under two water regimes, Full Irrigation (FI) and No Irrigation (NI).
25828510	3	30	theme	water	487:491	arg1	regimes					493:499	two water regimes	483:499	two water regimes	483:499	In the present work, berry texture, skin CW composition, and expression of remodeling genes were investigated in two V. vinifera varieties, Touriga Nacional (TN) and Trincadeira (TR), under two water regimes, Full Irrigation (FI) and No Irrigation (NI).
25828510	3	30	theme	water	487:491	arg1	Irrigation					507:516	Full Irrigation	502:516	Full Irrigation (FI)	502:521	In the present work, berry texture, skin CW composition, and expression of remodeling genes were investigated in two V. vinifera varieties, Touriga Nacional (TN) and Trincadeira (TR), under two water regimes, Full Irrigation (FI) and No Irrigation (NI).
25828510	3	31	theme	genes	379:383	arg1	expression					354:363	expression	354:363	expression of remodeling genes	354:383	In the present work, berry texture, skin CW composition, and expression of remodeling genes were investigated in two V. vinifera varieties, Touriga Nacional (TN) and Trincadeira (TR), under two water regimes, Full Irrigation (FI) and No Irrigation (NI).
25828510	3	31	theme	genes	379:383	arg1	texture					320:326	berry texture	314:326	berry texture	314:326	In the present work, berry texture, skin CW composition, and expression of remodeling genes were investigated in two V. vinifera varieties, Touriga Nacional (TN) and Trincadeira (TR), under two water regimes, Full Irrigation (FI) and No Irrigation (NI).
25828510	3	31	theme	genes	379:383	arg1	composition					337:347	skin CW composition	329:347	skin CW composition	329:347	In the present work, berry texture, skin CW composition, and expression of remodeling genes were investigated in two V. vinifera varieties, Touriga Nacional (TN) and Trincadeira (TR), under two water regimes, Full Irrigation (FI) and No Irrigation (NI).
25828510	0	32	theme	Skin	44:47	arg1	Composition					59:69	Skin Cell Wall Composition	44:69	Skin Cell Wall Composition	44:69	Relating Water Deficiency to Berry Texture, Skin Cell Wall Composition, and Expression of Remodeling Genes in Two Vitis vinifera L. Varieties.
25828510	0	33	theme	L.	129:130	arg1	Varieties					132:140	Two Vitis vinifera L. Varieties	110:140	Two Vitis vinifera L. Varieties	110:140	Relating Water Deficiency to Berry Texture, Skin Cell Wall Composition, and Expression of Remodeling Genes in Two Vitis vinifera L. Varieties.
25828510	3	34	theme	V.	410:411	arg1	Trincadeira					459:469	Trincadeira	459:469	Trincadeira	459:469	In the present work, berry texture, skin CW composition, and expression of remodeling genes were investigated in two V. vinifera varieties, Touriga Nacional (TN) and Trincadeira (TR), under two water regimes, Full Irrigation (FI) and No Irrigation (NI).
25828510	3	34	theme	V.	410:411	arg1	varieties					422:430	two V. vinifera varieties	406:430	two V. vinifera varieties	406:430	In the present work, berry texture, skin CW composition, and expression of remodeling genes were investigated in two V. vinifera varieties, Touriga Nacional (TN) and Trincadeira (TR), under two water regimes, Full Irrigation (FI) and No Irrigation (NI).
25828510	3	34	theme	V.	410:411	arg1	Nacional					441:448	Nacional	441:448	Nacional	441:448	In the present work, berry texture, skin CW composition, and expression of remodeling genes were investigated in two V. vinifera varieties, Touriga Nacional (TN) and Trincadeira (TR), under two water regimes, Full Irrigation (FI) and No Irrigation (NI).
25828510	3	35	theme	skin	329:332	arg1	composition					337:347	skin CW composition	329:347	skin CW composition	329:347	In the present work, berry texture, skin CW composition, and expression of remodeling genes were investigated in two V. vinifera varieties, Touriga Nacional (TN) and Trincadeira (TR), under two water regimes, Full Irrigation (FI) and No Irrigation (NI).
25828510	0	36	theme	vinifera	120:127	arg1	Varieties					132:140	Two Vitis vinifera L. Varieties	110:140	Two Vitis vinifera L. Varieties	110:140	Relating Water Deficiency to Berry Texture, Skin Cell Wall Composition, and Expression of Remodeling Genes in Two Vitis vinifera L. Varieties.
25828510	2	37	theme	sensorial	274:282	arg1	quality					284:290	its sensorial quality	270:290	its sensorial quality	270:290	Water shortage (WS) impacts grapevine berry composition and its sensorial quality.
25828510	3	38	theme	Full	502:505	arg1	FI					519:520	FI	519:520	FI	519:520	In the present work, berry texture, skin CW composition, and expression of remodeling genes were investigated in two V. vinifera varieties, Touriga Nacional (TN) and Trincadeira (TR), under two water regimes, Full Irrigation (FI) and No Irrigation (NI).
25828510	3	38	theme	Full	502:505	arg1	regimes					493:499	two water regimes	483:499	two water regimes	483:499	In the present work, berry texture, skin CW composition, and expression of remodeling genes were investigated in two V. vinifera varieties, Touriga Nacional (TN) and Trincadeira (TR), under two water regimes, Full Irrigation (FI) and No Irrigation (NI).
25828510	3	38	theme	Full	502:505	arg1	Irrigation					507:516	Full Irrigation	502:516	Full Irrigation (FI)	502:521	In the present work, berry texture, skin CW composition, and expression of remodeling genes were investigated in two V. vinifera varieties, Touriga Nacional (TN) and Trincadeira (TR), under two water regimes, Full Irrigation (FI) and No Irrigation (NI).
25828510	2	39	theme	Water	210:214	arg1	WS					226:227	WS	226:227	WS	226:227	Water shortage (WS) impacts grapevine berry composition and its sensorial quality.
25828510	2	39	theme	Water	210:214	arg1	shortage					216:223	Water shortage	210:223	Water shortage (WS)	210:228	Water shortage (WS) impacts grapevine berry composition and its sensorial quality.
25828510	4	40	theme	evident	577:583	arg1	separation					585:594	an evident separation	574:594	an evident separation between both varieties and the water treatments	574:642	The global results allowed an evident separation between both varieties and the water treatments.
25828510	1	41	theme	dynamic	167:173	arg1	wall					152:155	The cell wall	143:155	The cell wall (CW)	143:160	The cell wall (CW) is a dynamic structure that responds to stress.
25828510	1	41	theme	dynamic	167:173	arg1	structure					175:183	a dynamic structure	165:183	a dynamic structure that responds to stress	165:207	The cell wall (CW) is a dynamic structure that responds to stress.
25828510	3	42	theme	No	527:528	arg1	Irrigation					530:539	No Irrigation	527:539	No Irrigation (NI)	527:544	In the present work, berry texture, skin CW composition, and expression of remodeling genes were investigated in two V. vinifera varieties, Touriga Nacional (TN) and Trincadeira (TR), under two water regimes, Full Irrigation (FI) and No Irrigation (NI).
25828510	3	42	theme	No	527:528	arg1	regimes					493:499	two water regimes	483:499	two water regimes	483:499	In the present work, berry texture, skin CW composition, and expression of remodeling genes were investigated in two V. vinifera varieties, Touriga Nacional (TN) and Trincadeira (TR), under two water regimes, Full Irrigation (FI) and No Irrigation (NI).
25828510	3	42	theme	No	527:528	arg1	NI					542:543	NI	542:543	NI	542:543	In the present work, berry texture, skin CW composition, and expression of remodeling genes were investigated in two V. vinifera varieties, Touriga Nacional (TN) and Trincadeira (TR), under two water regimes, Full Irrigation (FI) and No Irrigation (NI).
25828510	6	43	theme	dependent	946:954	arg1	pattern					956:962	a variety dependent pattern	936:962	a variety dependent pattern	936:962	In response to WS, the majority of the CW related genes were down-regulated in a variety dependent pattern.
25828510	0	44	theme	Wall	54:57	arg1	Composition					59:69	Skin Cell Wall Composition	44:69	Skin Cell Wall Composition	44:69	Relating Water Deficiency to Berry Texture, Skin Cell Wall Composition, and Expression of Remodeling Genes in Two Vitis vinifera L. Varieties.
25828510	5	45	theme	reduced	710:716	arg1	amounts					718:724	reduced amounts	710:724	reduced amounts in cellulose and lignin at maturation	710:762	WS resulted in increased anthocyanin contents in both varieties, reduced amounts in cellulose and lignin at maturation, but an increase in arabinose-containing polysaccharides more tightly bound to the CW in TR.
25828510	6	46	theme	genes	907:911	arg1	majority					880:887	the majority	876:887	the majority of the CW related genes	876:911	In response to WS, the majority of the CW related genes were down-regulated in a variety dependent pattern.
25828510	0	47	theme	Cell	49:52	arg1	Composition					59:69	Skin Cell Wall Composition	44:69	Skin Cell Wall Composition	44:69	Relating Water Deficiency to Berry Texture, Skin Cell Wall Composition, and Expression of Remodeling Genes in Two Vitis vinifera L. Varieties.
25828510	5	48	from	amounts	718:724	arg1	maturation					753:762	maturation	753:762	maturation	753:762	WS resulted in increased anthocyanin contents in both varieties, reduced amounts in cellulose and lignin at maturation, but an increase in arabinose-containing polysaccharides more tightly bound to the CW in TR.
25828510	5	48	from	amounts	718:724	arg1	lignin					743:748	lignin	743:748	lignin	743:748	WS resulted in increased anthocyanin contents in both varieties, reduced amounts in cellulose and lignin at maturation, but an increase in arabinose-containing polysaccharides more tightly bound to the CW in TR.
25828510	5	48	from	amounts	718:724	arg1	cellulose					729:737	cellulose	729:737	cellulose	729:737	WS resulted in increased anthocyanin contents in both varieties, reduced amounts in cellulose and lignin at maturation, but an increase in arabinose-containing polysaccharides more tightly bound to the CW in TR.
25828510	3	49	theme	berry	314:318	arg1	texture					320:326	berry texture	314:326	berry texture	314:326	In the present work, berry texture, skin CW composition, and expression of remodeling genes were investigated in two V. vinifera varieties, Touriga Nacional (TN) and Trincadeira (TR), under two water regimes, Full Irrigation (FI) and No Irrigation (NI).
25828510	7	50	theme	grape	1016:1020	arg1	berries					1022:1028	grape berries	1016:1028	grape berries	1016:1028	The results support the assumption that WS affects grape berries by stiffening the CW through alteration in pectin structure, supporting its involvement in responses to environmental conditions.
30198660	5	0	dep	infrared	1054:1061	arg1	FTIR					1064:1067	FTIR	1064:1067	FTIR	1064:1067	Spectral analysis of all DOM samples was completed with the application of Fourier transform infrared (FTIR) spectrometer.
30198660	3	1	theme	main	691:694	arg1	purpose					696:702	The main purpose	687:702	The main purpose of this study	687:716	The main purpose of this study was to elucidate the change of structural composition of dissolved organic matter (DOM) in piggery wastewater during the process of organic degradation.
30198660	4	2	theme	dried	875:879	arg1	samples					895:901	All dried and solid DOM samples	871:901	All dried and solid DOM samples	871:901	All dried and solid DOM samples were achieved using filtration and freeze-drying methods.
30198660	7	3	theme	related	1405:1411	arg1	groups					1397:1402	functional groups	1386:1402	functional groups	1386:1402	With the increase in the incubation days, the percent of functional groups, related to proteins, phenolic acids, and lipids, decreased gradually and kept stable eventually, while these functional groups, linked with fulvic acids and polysaccharides, showed a significant increase and leveled off at the end.
30198660	9	4	theme	degradation	1836:1846	arg1	faster					1908:1913	faster	1908:1913	faster	1908:1913	Meanwhile, the degradation rate of OH bonded by intermolecular H-bond of cellulose was faster than OH bonded by intra-molecular H-bond of cellulose, whereas the latter was more sensitive to microbial degradation.
30198660	9	4	theme	degradation	1836:1846	arg1	rate					1848:1851	the degradation rate	1832:1851	the degradation rate of OH bonded by intermolecular H-bond of cellulose	1832:1902	Meanwhile, the degradation rate of OH bonded by intermolecular H-bond of cellulose was faster than OH bonded by intra-molecular H-bond of cellulose, whereas the latter was more sensitive to microbial degradation.
30198660	5	5	dep	Fourier	1036:1042	arg1	transform					1044:1052	transform	1044:1052	transform infrared (FTIR) spectrometer	1044:1081	Spectral analysis of all DOM samples was completed with the application of Fourier transform infrared (FTIR) spectrometer.
30198660	8	6	theme	DOM	1816:1818	arg1	degree					1806:1811	a higher aromatic degree	1788:1811	a higher aromatic degree of DOM	1788:1818	Compared with primary samples, fulvic acids and polysaccharides were the predominant fractions of DOM at 20 days after organic degradation, indicating a higher aromatic degree of DOM.
30198660	7	7	theme	groups	1397:1402	arg1	percent					1375:1381	the percent	1371:1381	the percent of functional groups, related to proteins, phenolic acids, and lipids,	1371:1452	With the increase in the incubation days, the percent of functional groups, related to proteins, phenolic acids, and lipids, decreased gradually and kept stable eventually, while these functional groups, linked with fulvic acids and polysaccharides, showed a significant increase and leveled off at the end.
30198660	10	8	theme	phenolic	2058:2065	arg1	C—O					2076:2078	phenolic hydroxyl C—O	2058:2078	phenolic hydroxyl C—O	2058:2078	The degradation rate of phenolic hydroxyl C—O was the fastest, followed by aromatic COOH, carbohydrate C—O, and amide CO.
30198660	3	9	theme	study	712:716	arg1	purpose					696:702	The main purpose	687:702	The main purpose of this study	687:716	The main purpose of this study was to elucidate the change of structural composition of dissolved organic matter (DOM) in piggery wastewater during the process of organic degradation.
30198660	0	10	theme	[Structural	0:10	arg1	Analysis					12:19	[Structural Analysis	0:19	[Structural Analysis of Organic Matter Composition in Piggery Wastewater during the Process of Organic Degradation	0:113	[Structural Analysis of Organic Matter Composition in Piggery Wastewater during the Process of Organic Degradation Based on FTIR Spectroscopy].
30198660	8	11	theme	fulvic	1668:1673	arg1	fractions					1722:1730	the predominant fractions	1706:1730	the predominant fractions of DOM	1706:1737	Compared with primary samples, fulvic acids and polysaccharides were the predominant fractions of DOM at 20 days after organic degradation, indicating a higher aromatic degree of DOM.
30198660	8	11	theme	fulvic	1668:1673	arg1	polysaccharides					1685:1699	polysaccharides	1685:1699	polysaccharides	1685:1699	Compared with primary samples, fulvic acids and polysaccharides were the predominant fractions of DOM at 20 days after organic degradation, indicating a higher aromatic degree of DOM.
30198660	8	11	theme	fulvic	1668:1673	arg1	acids					1675:1679	fulvic acids	1668:1679	fulvic acids	1668:1679	Compared with primary samples, fulvic acids and polysaccharides were the predominant fractions of DOM at 20 days after organic degradation, indicating a higher aromatic degree of DOM.
30198660	12	12	from	change	2270:2275	arg1	wastewater					2303:2312	piggery wastewater	2295:2312	piggery wastewater	2295:2312	In sum, the structural change of various DOM in piggery wastewater was different during the process of organic degradation.
30198660	2	13	theme	pig	569:571	arg1	farms					573:577	different scale pig farms	553:577	different scale pig farms	553:577	In the present study, a laboratory-scale incubation experiment was carried out with piggery wastewater collected from different scale pig farms, and a continuous sampling was conducted at a certain interval during the process of incubation experiment.
30198660	8	14	from	days	1745:1748	arg1	fractions					1722:1730	the predominant fractions	1706:1730	the predominant fractions of DOM	1706:1737	Compared with primary samples, fulvic acids and polysaccharides were the predominant fractions of DOM at 20 days after organic degradation, indicating a higher aromatic degree of DOM.
30198660	8	14	from	days	1745:1748	arg1	polysaccharides					1685:1699	polysaccharides	1685:1699	polysaccharides	1685:1699	Compared with primary samples, fulvic acids and polysaccharides were the predominant fractions of DOM at 20 days after organic degradation, indicating a higher aromatic degree of DOM.
30198660	8	14	from	days	1745:1748	arg1	acids					1675:1679	fulvic acids	1668:1679	fulvic acids	1668:1679	Compared with primary samples, fulvic acids and polysaccharides were the predominant fractions of DOM at 20 days after organic degradation, indicating a higher aromatic degree of DOM.
30198660	1	15	theme	environmental	186:198	arg1	pollutions					200:209	the environmental pollutions	182:209	the environmental pollutions of wastewater discharged from scale pig farms	182:255	More and more attentions were paid on the environmental pollutions of wastewater discharged from scale pig farms, and it could provide scientific bases for formulating reasonable pollution control measures to study the structural changes of organic matter composition in piggery wastewater.
30198660	7	16	theme	significant	1588:1598	arg1	increase					1600:1607	a significant increase	1586:1607	a significant increase	1586:1607	With the increase in the incubation days, the percent of functional groups, related to proteins, phenolic acids, and lipids, decreased gradually and kept stable eventually, while these functional groups, linked with fulvic acids and polysaccharides, showed a significant increase and leveled off at the end.
30198660	2	17	theme	different	553:561	arg1	farms					573:577	different scale pig farms	553:577	different scale pig farms	553:577	In the present study, a laboratory-scale incubation experiment was carried out with piggery wastewater collected from different scale pig farms, and a continuous sampling was conducted at a certain interval during the process of incubation experiment.
30198660	10	18	theme	C—O	2076:2078	arg1	rate					2050:2053	The degradation rate	2034:2053	The degradation rate of phenolic hydroxyl C—O	2034:2078	The degradation rate of phenolic hydroxyl C—O was the fastest, followed by aromatic COOH, carbohydrate C—O, and amide CO.
30198660	10	18	theme	C—O	2076:2078	arg1	fastest					2088:2094	fastest	2088:2094	fastest	2088:2094	The degradation rate of phenolic hydroxyl C—O was the fastest, followed by aromatic COOH, carbohydrate C—O, and amide CO.
30198660	3	19	theme	dissolved	775:783	arg1	DOM					801:803	DOM	801:803	DOM	801:803	The main purpose of this study was to elucidate the change of structural composition of dissolved organic matter (DOM) in piggery wastewater during the process of organic degradation.
30198660	3	19	theme	dissolved	775:783	arg1	matter					793:798	dissolved organic matter	775:798	dissolved organic matter (DOM)	775:804	The main purpose of this study was to elucidate the change of structural composition of dissolved organic matter (DOM) in piggery wastewater during the process of organic degradation.
30198660	1	20	theme	matter	393:398	arg1	composition					400:410	organic matter composition	385:410	organic matter composition	385:410	More and more attentions were paid on the environmental pollutions of wastewater discharged from scale pig farms, and it could provide scientific bases for formulating reasonable pollution control measures to study the structural changes of organic matter composition in piggery wastewater.
30198660	8	21	theme	predominant	1710:1720	arg1	fractions					1722:1730	the predominant fractions	1706:1730	the predominant fractions of DOM	1706:1737	Compared with primary samples, fulvic acids and polysaccharides were the predominant fractions of DOM at 20 days after organic degradation, indicating a higher aromatic degree of DOM.
30198660	8	21	theme	predominant	1710:1720	arg1	polysaccharides					1685:1699	polysaccharides	1685:1699	polysaccharides	1685:1699	Compared with primary samples, fulvic acids and polysaccharides were the predominant fractions of DOM at 20 days after organic degradation, indicating a higher aromatic degree of DOM.
30198660	8	21	theme	predominant	1710:1720	arg1	acids					1675:1679	fulvic acids	1668:1679	fulvic acids	1668:1679	Compared with primary samples, fulvic acids and polysaccharides were the predominant fractions of DOM at 20 days after organic degradation, indicating a higher aromatic degree of DOM.
30198660	4	22	theme	solid	885:889	arg1	samples					895:901	All dried and solid DOM samples	871:901	All dried and solid DOM samples	871:901	All dried and solid DOM samples were achieved using filtration and freeze-drying methods.
30198660	0	23	from	Wastewater	62:71	arg1	Analysis					12:19	[Structural Analysis	0:19	[Structural Analysis of Organic Matter Composition in Piggery Wastewater during the Process of Organic Degradation	0:113	[Structural Analysis of Organic Matter Composition in Piggery Wastewater during the Process of Organic Degradation Based on FTIR Spectroscopy].
30198660	3	24	theme	composition	760:770	arg1	change					739:744	the change	735:744	the change of structural composition of dissolved organic matter (DOM) in piggery wastewater	735:826	The main purpose of this study was to elucidate the change of structural composition of dissolved organic matter (DOM) in piggery wastewater during the process of organic degradation.
30198660	5	25	theme	Fourier	1036:1042	arg1	application					1021:1031	the application	1017:1031	the application of Fourier transform infrared (FTIR) spectrometer	1017:1081	Spectral analysis of all DOM samples was completed with the application of Fourier transform infrared (FTIR) spectrometer.
30198660	6	26	theme	pig	1217:1219	arg1	farms					1221:1225	different scale pig farms	1201:1225	different scale pig farms	1201:1225	Results of spectral analysis showed a similar DOM structural composition was observed in the wastewater derived from different scale pig farms, and was mainly comprised of lipids, proteins, fulvic acids, polysaccharides, and phenolic compounds.
30198660	10	27	theme	aromatic	2109:2116	arg1	COOH					2118:2121	aromatic COOH	2109:2121	aromatic COOH	2109:2121	The degradation rate of phenolic hydroxyl C—O was the fastest, followed by aromatic COOH, carbohydrate C—O, and amide CO.
30198660	12	28	from	different	2318:2326	arg1	sum					2250:2252	sum	2250:2252	sum	2250:2252	In sum, the structural change of various DOM in piggery wastewater was different during the process of organic degradation.
30198660	5	29	theme	infrared	1054:1061	arg1	spectrometer					1070:1081	infrared (FTIR) spectrometer	1054:1081	infrared (FTIR) spectrometer	1054:1081	Spectral analysis of all DOM samples was completed with the application of Fourier transform infrared (FTIR) spectrometer.
30198660	6	30	theme	different	1201:1209	arg1	farms					1221:1225	different scale pig farms	1201:1225	different scale pig farms	1201:1225	Results of spectral analysis showed a similar DOM structural composition was observed in the wastewater derived from different scale pig farms, and was mainly comprised of lipids, proteins, fulvic acids, polysaccharides, and phenolic compounds.
30198660	10	31	theme	carbohydrate	2124:2135	arg1	C—O					2137:2139	carbohydrate C—O	2124:2139	carbohydrate C—O	2124:2139	The degradation rate of phenolic hydroxyl C—O was the fastest, followed by aromatic COOH, carbohydrate C—O, and amide CO.
30198660	3	32	theme	matter	793:798	arg1	composition					760:770	structural composition	749:770	structural composition of dissolved organic matter (DOM)	749:804	The main purpose of this study was to elucidate the change of structural composition of dissolved organic matter (DOM) in piggery wastewater during the process of organic degradation.
30198660	12	33	theme	DOM	2288:2290	arg1	change					2270:2275	the structural change	2255:2275	the structural change of various DOM in piggery wastewater	2255:2312	In sum, the structural change of various DOM in piggery wastewater was different during the process of organic degradation.
30198660	12	33	theme	DOM	2288:2290	arg1	different					2318:2326	different	2318:2326	different	2318:2326	In sum, the structural change of various DOM in piggery wastewater was different during the process of organic degradation.
30198660	1	34	theme	pollution	323:331	arg1	measures					341:348	reasonable pollution control measures	312:348	reasonable pollution control measures	312:348	More and more attentions were paid on the environmental pollutions of wastewater discharged from scale pig farms, and it could provide scientific bases for formulating reasonable pollution control measures to study the structural changes of organic matter composition in piggery wastewater.
30198660	1	35	theme	More	144:147	arg1	attentions					158:167	More and more attentions	144:167	More and more attentions	144:167	More and more attentions were paid on the environmental pollutions of wastewater discharged from scale pig farms, and it could provide scientific bases for formulating reasonable pollution control measures to study the structural changes of organic matter composition in piggery wastewater.
30198660	8	36	theme	organic	1756:1762	arg1	degradation					1764:1774	organic degradation	1756:1774	organic degradation	1756:1774	Compared with primary samples, fulvic acids and polysaccharides were the predominant fractions of DOM at 20 days after organic degradation, indicating a higher aromatic degree of DOM.
30198660	1	37	theme	more	153:156	arg1	attentions					158:167	More and more attentions	144:167	More and more attentions	144:167	More and more attentions were paid on the environmental pollutions of wastewater discharged from scale pig farms, and it could provide scientific bases for formulating reasonable pollution control measures to study the structural changes of organic matter composition in piggery wastewater.
30198660	3	38	theme	degradation	858:868	arg1	process					839:845	the process	835:845	the process of organic degradation	835:868	The main purpose of this study was to elucidate the change of structural composition of dissolved organic matter (DOM) in piggery wastewater during the process of organic degradation.
30198660	10	39	theme	amide	2146:2150	arg1	CO					2152:2153	amide CO	2146:2153	amide CO	2146:2153	The degradation rate of phenolic hydroxyl C—O was the fastest, followed by aromatic COOH, carbohydrate C—O, and amide CO.
30198660	7	40	theme	functional	1514:1523	arg1	groups					1525:1530	these functional groups	1508:1530	these functional groups	1508:1530	With the increase in the incubation days, the percent of functional groups, related to proteins, phenolic acids, and lipids, decreased gradually and kept stable eventually, while these functional groups, linked with fulvic acids and polysaccharides, showed a significant increase and leveled off at the end.
30198660	2	41	theme	piggery	519:525	arg1	collected					538:546	piggery wastewater collected	519:546	piggery wastewater collected from different scale pig farms	519:577	In the present study, a laboratory-scale incubation experiment was carried out with piggery wastewater collected from different scale pig farms, and a continuous sampling was conducted at a certain interval during the process of incubation experiment.
30198660	0	42	theme	Degradation	103:113	arg1	Process					84:90	the Process	80:90	the Process of Organic Degradation	80:113	[Structural Analysis of Organic Matter Composition in Piggery Wastewater during the Process of Organic Degradation Based on FTIR Spectroscopy].
30198660	1	43	theme	structural	363:372	arg1	changes					374:380	the structural changes	359:380	the structural changes of organic matter composition in piggery wastewater	359:432	More and more attentions were paid on the environmental pollutions of wastewater discharged from scale pig farms, and it could provide scientific bases for formulating reasonable pollution control measures to study the structural changes of organic matter composition in piggery wastewater.
30198660	7	44	from	increase	1338:1345	arg1	days					1365:1368	the incubation days	1350:1368	the incubation days	1350:1368	With the increase in the incubation days, the percent of functional groups, related to proteins, phenolic acids, and lipids, decreased gradually and kept stable eventually, while these functional groups, linked with fulvic acids and polysaccharides, showed a significant increase and leveled off at the end.
30198660	1	45	from	changes	374:380	arg1	wastewater					423:432	piggery wastewater	415:432	piggery wastewater	415:432	More and more attentions were paid on the environmental pollutions of wastewater discharged from scale pig farms, and it could provide scientific bases for formulating reasonable pollution control measures to study the structural changes of organic matter composition in piggery wastewater.
30198660	8	46	theme	aromatic	1797:1804	arg1	degree					1806:1811	a higher aromatic degree	1788:1811	a higher aromatic degree of DOM	1788:1818	Compared with primary samples, fulvic acids and polysaccharides were the predominant fractions of DOM at 20 days after organic degradation, indicating a higher aromatic degree of DOM.
30198660	2	47	theme	incubation	664:673	arg1	experiment					675:684	incubation experiment	664:684	incubation experiment	664:684	In the present study, a laboratory-scale incubation experiment was carried out with piggery wastewater collected from different scale pig farms, and a continuous sampling was conducted at a certain interval during the process of incubation experiment.
30198660	12	48	from	sum	2250:2252	arg1	different					2318:2326	different	2318:2326	different	2318:2326	In sum, the structural change of various DOM in piggery wastewater was different during the process of organic degradation.
30198660	12	48	from	sum	2250:2252	arg1	change					2270:2275	the structural change	2255:2275	the structural change of various DOM in piggery wastewater	2255:2312	In sum, the structural change of various DOM in piggery wastewater was different during the process of organic degradation.
30198660	2	49	theme	incubation	476:485	arg1	experiment					487:496	a laboratory-scale incubation experiment	457:496	a laboratory-scale incubation experiment	457:496	In the present study, a laboratory-scale incubation experiment was carried out with piggery wastewater collected from different scale pig farms, and a continuous sampling was conducted at a certain interval during the process of incubation experiment.
30198660	12	50	theme	degradation	2358:2368	arg1	process					2339:2345	the process	2335:2345	the process of organic degradation	2335:2368	In sum, the structural change of various DOM in piggery wastewater was different during the process of organic degradation.
30198660	0	51	theme	Matter	32:37	arg1	Composition					39:49	Organic Matter Composition	24:49	Organic Matter Composition in Piggery Wastewater	24:71	[Structural Analysis of Organic Matter Composition in Piggery Wastewater during the Process of Organic Degradation Based on FTIR Spectroscopy].
30198660	9	52	theme	microbial	2011:2019	arg1	degradation					2021:2031	microbial degradation	2011:2031	microbial degradation	2011:2031	Meanwhile, the degradation rate of OH bonded by intermolecular H-bond of cellulose was faster than OH bonded by intra-molecular H-bond of cellulose, whereas the latter was more sensitive to microbial degradation.
30198660	6	53	theme	similar	1122:1128	arg1	composition					1145:1155	a similar DOM structural composition	1120:1155	a similar DOM structural composition	1120:1155	Results of spectral analysis showed a similar DOM structural composition was observed in the wastewater derived from different scale pig farms, and was mainly comprised of lipids, proteins, fulvic acids, polysaccharides, and phenolic compounds.
30198660	1	54	theme	wastewater	214:223	arg1	pollutions					200:209	the environmental pollutions	182:209	the environmental pollutions of wastewater discharged from scale pig farms	182:255	More and more attentions were paid on the environmental pollutions of wastewater discharged from scale pig farms, and it could provide scientific bases for formulating reasonable pollution control measures to study the structural changes of organic matter composition in piggery wastewater.
30198660	5	55	theme	samples	990:996	arg1	analysis					970:977	Spectral analysis	961:977	Spectral analysis of all DOM samples	961:996	Spectral analysis of all DOM samples was completed with the application of Fourier transform infrared (FTIR) spectrometer.
30198660	11	56	theme	carbohydrate	2173:2184	arg1	C—O					2186:2188	the carbohydrate C—O	2169:2188	the carbohydrate C—O	2169:2188	Furthermore, the carbohydrate C—O was apt to be utilized preferentially by microorganisms.
30198660	4	57	dep	filtration	923:932	arg1	methods					952:958	methods	952:958	methods	952:958	All dried and solid DOM samples were achieved using filtration and freeze-drying methods.
30198660	6	58	theme	spectral	1095:1102	arg1	analysis					1104:1111	spectral analysis	1095:1111	spectral analysis	1095:1111	Results of spectral analysis showed a similar DOM structural composition was observed in the wastewater derived from different scale pig farms, and was mainly comprised of lipids, proteins, fulvic acids, polysaccharides, and phenolic compounds.
30198660	10	59	theme	degradation	2038:2048	arg1	rate					2050:2053	The degradation rate	2034:2053	The degradation rate of phenolic hydroxyl C—O	2034:2078	The degradation rate of phenolic hydroxyl C—O was the fastest, followed by aromatic COOH, carbohydrate C—O, and amide CO.
30198660	10	59	theme	degradation	2038:2048	arg1	fastest					2088:2094	fastest	2088:2094	fastest	2088:2094	The degradation rate of phenolic hydroxyl C—O was the fastest, followed by aromatic COOH, carbohydrate C—O, and amide CO.
30198660	6	60	theme	fulvic	1274:1279	arg1	acids					1281:1285	fulvic acids	1274:1285	fulvic acids	1274:1285	Results of spectral analysis showed a similar DOM structural composition was observed in the wastewater derived from different scale pig farms, and was mainly comprised of lipids, proteins, fulvic acids, polysaccharides, and phenolic compounds.
30198660	1	61	theme	pig	247:249	arg1	farms					251:255	scale pig farms	241:255	scale pig farms	241:255	More and more attentions were paid on the environmental pollutions of wastewater discharged from scale pig farms, and it could provide scientific bases for formulating reasonable pollution control measures to study the structural changes of organic matter composition in piggery wastewater.
30198660	2	62	from	farms	573:577	arg1	collected					538:546	piggery wastewater collected	519:546	piggery wastewater collected from different scale pig farms	519:577	In the present study, a laboratory-scale incubation experiment was carried out with piggery wastewater collected from different scale pig farms, and a continuous sampling was conducted at a certain interval during the process of incubation experiment.
30198660	7	63	theme	phenolic	1426:1433	arg1	acids					1435:1439	phenolic acids	1426:1439	phenolic acids	1426:1439	With the increase in the incubation days, the percent of functional groups, related to proteins, phenolic acids, and lipids, decreased gradually and kept stable eventually, while these functional groups, linked with fulvic acids and polysaccharides, showed a significant increase and leveled off at the end.
30198660	2	64	theme	continuous	586:595	arg1	sampling					597:604	a continuous sampling	584:604	a continuous sampling	584:604	In the present study, a laboratory-scale incubation experiment was carried out with piggery wastewater collected from different scale pig farms, and a continuous sampling was conducted at a certain interval during the process of incubation experiment.
30198660	10	65	theme	hydroxyl	2067:2074	arg1	C—O					2076:2078	phenolic hydroxyl C—O	2058:2078	phenolic hydroxyl C—O	2058:2078	The degradation rate of phenolic hydroxyl C—O was the fastest, followed by aromatic COOH, carbohydrate C—O, and amide CO.
30198660	12	66	theme	various	2280:2286	arg1	DOM					2288:2290	various DOM	2280:2290	various DOM	2280:2290	In sum, the structural change of various DOM in piggery wastewater was different during the process of organic degradation.
30198660	8	67	theme	primary	1651:1657	arg1	samples					1659:1665	primary samples	1651:1665	primary samples	1651:1665	Compared with primary samples, fulvic acids and polysaccharides were the predominant fractions of DOM at 20 days after organic degradation, indicating a higher aromatic degree of DOM.
30198660	2	68	theme	certain	625:631	arg1	interval					633:640	a certain interval	623:640	a certain interval	623:640	In the present study, a laboratory-scale incubation experiment was carried out with piggery wastewater collected from different scale pig farms, and a continuous sampling was conducted at a certain interval during the process of incubation experiment.
30198660	9	69	theme	OH	1856:1857	arg1	faster					1908:1913	faster	1908:1913	faster	1908:1913	Meanwhile, the degradation rate of OH bonded by intermolecular H-bond of cellulose was faster than OH bonded by intra-molecular H-bond of cellulose, whereas the latter was more sensitive to microbial degradation.
30198660	9	69	theme	OH	1856:1857	arg1	rate					1848:1851	the degradation rate	1832:1851	the degradation rate of OH bonded by intermolecular H-bond of cellulose	1832:1902	Meanwhile, the degradation rate of OH bonded by intermolecular H-bond of cellulose was faster than OH bonded by intra-molecular H-bond of cellulose, whereas the latter was more sensitive to microbial degradation.
30198660	7	70	theme	functional	1386:1395	arg1	groups					1397:1402	functional groups	1386:1402	functional groups	1386:1402	With the increase in the incubation days, the percent of functional groups, related to proteins, phenolic acids, and lipids, decreased gradually and kept stable eventually, while these functional groups, linked with fulvic acids and polysaccharides, showed a significant increase and leveled off at the end.
30198660	3	71	theme	structural	749:758	arg1	composition					760:770	structural composition	749:770	structural composition of dissolved organic matter (DOM)	749:804	The main purpose of this study was to elucidate the change of structural composition of dissolved organic matter (DOM) in piggery wastewater during the process of organic degradation.
30198660	0	72	theme	FTIR	124:127	arg1	Spectroscopy					129:140	FTIR Spectroscopy	124:140	FTIR Spectroscopy	124:140	[Structural Analysis of Organic Matter Composition in Piggery Wastewater during the Process of Organic Degradation Based on FTIR Spectroscopy].
30198660	2	73	theme	scale	563:567	arg1	farms					573:577	different scale pig farms	553:577	different scale pig farms	553:577	In the present study, a laboratory-scale incubation experiment was carried out with piggery wastewater collected from different scale pig farms, and a continuous sampling was conducted at a certain interval during the process of incubation experiment.
30198660	6	74	theme	structural	1134:1143	arg1	composition					1145:1155	a similar DOM structural composition	1120:1155	a similar DOM structural composition	1120:1155	Results of spectral analysis showed a similar DOM structural composition was observed in the wastewater derived from different scale pig farms, and was mainly comprised of lipids, proteins, fulvic acids, polysaccharides, and phenolic compounds.
30198660	6	75	theme	phenolic	1309:1316	arg1	compounds					1318:1326	phenolic compounds	1309:1326	phenolic compounds	1309:1326	Results of spectral analysis showed a similar DOM structural composition was observed in the wastewater derived from different scale pig farms, and was mainly comprised of lipids, proteins, fulvic acids, polysaccharides, and phenolic compounds.
30198660	1	76	theme	organic	385:391	arg1	composition					400:410	organic matter composition	385:410	organic matter composition	385:410	More and more attentions were paid on the environmental pollutions of wastewater discharged from scale pig farms, and it could provide scientific bases for formulating reasonable pollution control measures to study the structural changes of organic matter composition in piggery wastewater.
30198660	12	77	theme	structural	2259:2268	arg1	change					2270:2275	the structural change	2255:2275	the structural change of various DOM in piggery wastewater	2255:2312	In sum, the structural change of various DOM in piggery wastewater was different during the process of organic degradation.
30198660	12	77	theme	structural	2259:2268	arg1	different					2318:2326	different	2318:2326	different	2318:2326	In sum, the structural change of various DOM in piggery wastewater was different during the process of organic degradation.
30198660	9	78	theme	cellulose	1894:1902	arg1	H-bond					1884:1889	H-bond	1884:1889	H-bond	1884:1889	Meanwhile, the degradation rate of OH bonded by intermolecular H-bond of cellulose was faster than OH bonded by intra-molecular H-bond of cellulose, whereas the latter was more sensitive to microbial degradation.
30198660	3	79	theme	organic	785:791	arg1	DOM					801:803	DOM	801:803	DOM	801:803	The main purpose of this study was to elucidate the change of structural composition of dissolved organic matter (DOM) in piggery wastewater during the process of organic degradation.
30198660	3	79	theme	organic	785:791	arg1	matter					793:798	dissolved organic matter	775:798	dissolved organic matter (DOM)	775:804	The main purpose of this study was to elucidate the change of structural composition of dissolved organic matter (DOM) in piggery wastewater during the process of organic degradation.
30198660	1	80	theme	composition	400:410	arg1	changes					374:380	the structural changes	359:380	the structural changes of organic matter composition in piggery wastewater	359:432	More and more attentions were paid on the environmental pollutions of wastewater discharged from scale pig farms, and it could provide scientific bases for formulating reasonable pollution control measures to study the structural changes of organic matter composition in piggery wastewater.
30198660	7	81	theme	incubation	1354:1363	arg1	days					1365:1368	the incubation days	1350:1368	the incubation days	1350:1368	With the increase in the incubation days, the percent of functional groups, related to proteins, phenolic acids, and lipids, decreased gradually and kept stable eventually, while these functional groups, linked with fulvic acids and polysaccharides, showed a significant increase and leveled off at the end.
30198660	1	82	theme	piggery	415:421	arg1	wastewater					423:432	piggery wastewater	415:432	piggery wastewater	415:432	More and more attentions were paid on the environmental pollutions of wastewater discharged from scale pig farms, and it could provide scientific bases for formulating reasonable pollution control measures to study the structural changes of organic matter composition in piggery wastewater.
30198660	3	83	theme	organic	850:856	arg1	degradation					858:868	organic degradation	850:868	organic degradation	850:868	The main purpose of this study was to elucidate the change of structural composition of dissolved organic matter (DOM) in piggery wastewater during the process of organic degradation.
30198660	7	84	theme	fulvic	1545:1550	arg1	acids					1552:1556	fulvic acids	1545:1556	fulvic acids	1545:1556	With the increase in the incubation days, the percent of functional groups, related to proteins, phenolic acids, and lipids, decreased gradually and kept stable eventually, while these functional groups, linked with fulvic acids and polysaccharides, showed a significant increase and leveled off at the end.
30198660	4	85	theme	DOM	891:893	arg1	samples					895:901	All dried and solid DOM samples	871:901	All dried and solid DOM samples	871:901	All dried and solid DOM samples were achieved using filtration and freeze-drying methods.
30198660	6	86	theme	scale	1211:1215	arg1	farms					1221:1225	different scale pig farms	1201:1225	different scale pig farms	1201:1225	Results of spectral analysis showed a similar DOM structural composition was observed in the wastewater derived from different scale pig farms, and was mainly comprised of lipids, proteins, fulvic acids, polysaccharides, and phenolic compounds.
30198660	8	87	theme	DOM	1735:1737	arg1	fractions					1722:1730	the predominant fractions	1706:1730	the predominant fractions of DOM	1706:1737	Compared with primary samples, fulvic acids and polysaccharides were the predominant fractions of DOM at 20 days after organic degradation, indicating a higher aromatic degree of DOM.
30198660	8	87	theme	DOM	1735:1737	arg1	polysaccharides					1685:1699	polysaccharides	1685:1699	polysaccharides	1685:1699	Compared with primary samples, fulvic acids and polysaccharides were the predominant fractions of DOM at 20 days after organic degradation, indicating a higher aromatic degree of DOM.
30198660	8	87	theme	DOM	1735:1737	arg1	acids					1675:1679	fulvic acids	1668:1679	fulvic acids	1668:1679	Compared with primary samples, fulvic acids and polysaccharides were the predominant fractions of DOM at 20 days after organic degradation, indicating a higher aromatic degree of DOM.
30198660	1	88	theme	control	333:339	arg1	measures					341:348	reasonable pollution control measures	312:348	reasonable pollution control measures	312:348	More and more attentions were paid on the environmental pollutions of wastewater discharged from scale pig farms, and it could provide scientific bases for formulating reasonable pollution control measures to study the structural changes of organic matter composition in piggery wastewater.
30198660	0	89	from	Analysis	12:19	arg1	Wastewater					62:71	Piggery Wastewater	54:71	Piggery Wastewater	54:71	[Structural Analysis of Organic Matter Composition in Piggery Wastewater during the Process of Organic Degradation Based on FTIR Spectroscopy].
30198660	6	90	attach	derived	1188:1194	arg2	wastewater					1177:1186	the wastewater	1173:1186	the wastewater derived from different scale pig farms	1173:1225	Results of spectral analysis showed a similar DOM structural composition was observed in the wastewater derived from different scale pig farms, and was mainly comprised of lipids, proteins, fulvic acids, polysaccharides, and phenolic compounds.
30198660	6	90	attach	derived	1188:1194	arg1	farms					1221:1225	different scale pig farms	1201:1225	different scale pig farms	1201:1225	Results of spectral analysis showed a similar DOM structural composition was observed in the wastewater derived from different scale pig farms, and was mainly comprised of lipids, proteins, fulvic acids, polysaccharides, and phenolic compounds.
30198660	0	91	theme	Organic	95:101	arg1	Degradation					103:113	Organic Degradation	95:113	Organic Degradation	95:113	[Structural Analysis of Organic Matter Composition in Piggery Wastewater during the Process of Organic Degradation Based on FTIR Spectroscopy].
30198660	5	92	theme	Spectral	961:968	arg1	analysis					970:977	Spectral analysis	961:977	Spectral analysis of all DOM samples	961:996	Spectral analysis of all DOM samples was completed with the application of Fourier transform infrared (FTIR) spectrometer.
30198660	2	93	theme	wastewater	527:536	arg1	collected					538:546	piggery wastewater collected	519:546	piggery wastewater collected from different scale pig farms	519:577	In the present study, a laboratory-scale incubation experiment was carried out with piggery wastewater collected from different scale pig farms, and a continuous sampling was conducted at a certain interval during the process of incubation experiment.
30198660	9	94	theme	cellulose	1959:1967	arg1	H-bond					1949:1954	H-bond	1949:1954	H-bond	1949:1954	Meanwhile, the degradation rate of OH bonded by intermolecular H-bond of cellulose was faster than OH bonded by intra-molecular H-bond of cellulose, whereas the latter was more sensitive to microbial degradation.
30198660	3	95	theme	piggery	809:815	arg1	wastewater					817:826	piggery wastewater	809:826	piggery wastewater	809:826	The main purpose of this study was to elucidate the change of structural composition of dissolved organic matter (DOM) in piggery wastewater during the process of organic degradation.
30198660	0	96	from	Composition	39:49	arg1	Wastewater					62:71	Piggery Wastewater	54:71	Piggery Wastewater	54:71	[Structural Analysis of Organic Matter Composition in Piggery Wastewater during the Process of Organic Degradation Based on FTIR Spectroscopy].
30198660	3	97	from	change	739:744	arg1	wastewater					817:826	piggery wastewater	809:826	piggery wastewater	809:826	The main purpose of this study was to elucidate the change of structural composition of dissolved organic matter (DOM) in piggery wastewater during the process of organic degradation.
30198660	2	98	theme	present	442:448	arg1	study					450:454	the present study	438:454	the present study	438:454	In the present study, a laboratory-scale incubation experiment was carried out with piggery wastewater collected from different scale pig farms, and a continuous sampling was conducted at a certain interval during the process of incubation experiment.
30198660	12	99	theme	organic	2350:2356	arg1	degradation					2358:2368	organic degradation	2350:2368	organic degradation	2350:2368	In sum, the structural change of various DOM in piggery wastewater was different during the process of organic degradation.
30198660	2	100	theme	experiment	675:684	arg1	process					653:659	the process	649:659	the process of incubation experiment	649:684	In the present study, a laboratory-scale incubation experiment was carried out with piggery wastewater collected from different scale pig farms, and a continuous sampling was conducted at a certain interval during the process of incubation experiment.
30198660	0	101	theme	Organic	24:30	arg1	Composition					39:49	Organic Matter Composition	24:49	Organic Matter Composition in Piggery Wastewater	24:71	[Structural Analysis of Organic Matter Composition in Piggery Wastewater during the Process of Organic Degradation Based on FTIR Spectroscopy].
30198660	8	102	theme	higher	1790:1795	arg1	degree					1806:1811	a higher aromatic degree	1788:1811	a higher aromatic degree of DOM	1788:1818	Compared with primary samples, fulvic acids and polysaccharides were the predominant fractions of DOM at 20 days after organic degradation, indicating a higher aromatic degree of DOM.
30198660	0	103	theme	Composition	39:49	arg1	Analysis					12:19	[Structural Analysis	0:19	[Structural Analysis of Organic Matter Composition in Piggery Wastewater during the Process of Organic Degradation	0:113	[Structural Analysis of Organic Matter Composition in Piggery Wastewater during the Process of Organic Degradation Based on FTIR Spectroscopy].
30198660	1	104	theme	scientific	279:288	arg1	bases					290:294	scientific bases	279:294	scientific bases	279:294	More and more attentions were paid on the environmental pollutions of wastewater discharged from scale pig farms, and it could provide scientific bases for formulating reasonable pollution control measures to study the structural changes of organic matter composition in piggery wastewater.
30198660	2	105	theme	laboratory-scale	459:474	arg1	experiment					487:496	a laboratory-scale incubation experiment	457:496	a laboratory-scale incubation experiment	457:496	In the present study, a laboratory-scale incubation experiment was carried out with piggery wastewater collected from different scale pig farms, and a continuous sampling was conducted at a certain interval during the process of incubation experiment.
30198660	0	106	theme	Piggery	54:60	arg1	Wastewater					62:71	Piggery Wastewater	54:71	Piggery Wastewater	54:71	[Structural Analysis of Organic Matter Composition in Piggery Wastewater during the Process of Organic Degradation Based on FTIR Spectroscopy].
30198660	6	107	theme	DOM	1130:1132	arg1	composition					1145:1155	a similar DOM structural composition	1120:1155	a similar DOM structural composition	1120:1155	Results of spectral analysis showed a similar DOM structural composition was observed in the wastewater derived from different scale pig farms, and was mainly comprised of lipids, proteins, fulvic acids, polysaccharides, and phenolic compounds.
30198660	1	108	theme	reasonable	312:321	arg1	measures					341:348	reasonable pollution control measures	312:348	reasonable pollution control measures	312:348	More and more attentions were paid on the environmental pollutions of wastewater discharged from scale pig farms, and it could provide scientific bases for formulating reasonable pollution control measures to study the structural changes of organic matter composition in piggery wastewater.
30198660	5	109	theme	DOM	986:988	arg1	samples					990:996	all DOM samples	982:996	all DOM samples	982:996	Spectral analysis of all DOM samples was completed with the application of Fourier transform infrared (FTIR) spectrometer.
30198660	12	110	theme	piggery	2295:2301	arg1	wastewater					2303:2312	piggery wastewater	2295:2312	piggery wastewater	2295:2312	In sum, the structural change of various DOM in piggery wastewater was different during the process of organic degradation.
30198660	6	111	dep	showed	1113:1118	arg1	observed					1161:1168	observed	1161:1168	was observed in the wastewater derived from different scale pig farms	1157:1225	Results of spectral analysis showed a similar DOM structural composition was observed in the wastewater derived from different scale pig farms, and was mainly comprised of lipids, proteins, fulvic acids, polysaccharides, and phenolic compounds.
30198660	6	111	dep	showed	1113:1118	arg1	comprised					1243:1251	comprised	1243:1251	was mainly comprised of lipids, proteins, fulvic acids, polysaccharides, and phenolic compounds	1232:1326	Results of spectral analysis showed a similar DOM structural composition was observed in the wastewater derived from different scale pig farms, and was mainly comprised of lipids, proteins, fulvic acids, polysaccharides, and phenolic compounds.
30198660	6	112	theme	analysis	1104:1111	arg1	Results					1084:1090	Results	1084:1090	Results of spectral analysis	1084:1111	Results of spectral analysis showed a similar DOM structural composition was observed in the wastewater derived from different scale pig farms, and was mainly comprised of lipids, proteins, fulvic acids, polysaccharides, and phenolic compounds.
30198660	11	113	used	utilized	2204:2211	arg2	apt					2194:2196	apt	2194:2196	apt	2194:2196	Furthermore, the carbohydrate C—O was apt to be utilized preferentially by microorganisms.
30198660	1	114	theme	scale	241:245	arg1	farms					251:255	scale pig farms	241:255	scale pig farms	241:255	More and more attentions were paid on the environmental pollutions of wastewater discharged from scale pig farms, and it could provide scientific bases for formulating reasonable pollution control measures to study the structural changes of organic matter composition in piggery wastewater.
24740695	6	0	theme	potential	1198:1206	arg1	applications					1225:1236	potential biotechnological applications	1198:1236	potential biotechnological applications in the environmental field	1198:1263	We also demonstrate that AaPs were able to remove phenolic compounds from olive oil mill wastewaters with a higher catalytic efficiency with respect to horseradish peroxidase, thus representing candidate enzymes for potential biotechnological applications in the environmental field.
24740695	4	1	contain	containing	727:736	arg2	%					769:769	21.75, 22.27, 25.62, and 18.31 %	738:769	21.75, 22.27, 25.62, and 18.31 % of carbohydrates	738:786	In addition, AaP-1, AaP-2, AaP-3, and AaP-4 were found to be glycoproteins, containing 21.75, 22.27, 25.62, and 18.31 % of carbohydrates, respectively.
24740695	4	1	contain	containing	727:736	arg2	carbohydrates					774:786	carbohydrates	774:786	carbohydrates	774:786	In addition, AaP-1, AaP-2, AaP-3, and AaP-4 were found to be glycoproteins, containing 21.75, 22.27, 25.62, and 18.31 % of carbohydrates, respectively.
24740695	4	1	contain	containing	727:736	arg1	glycoproteins					712:724	glycoproteins	712:724	glycoproteins	712:724	In addition, AaP-1, AaP-2, AaP-3, and AaP-4 were found to be glycoproteins, containing 21.75, 22.27, 25.62, and 18.31 % of carbohydrates, respectively.
24740695	2	2	theme	41595.5	452:458	arg1	values					411:416	different Mr values	398:416	different Mr values of 42758.3, 41586.9, 42796.3, and 41595.5	398:458	The four proteins showed a similar electrophoretic mobility of 46 kDa while, by MALDI-TOF MS, different Mr values of 42758.3, 41586.9, 42796.3, and 41595.5 were determined for AaP-1, AaP-2, AaP-3, and AaP-4, respectively.
24740695	4	3	located	found	700:704	arg2	AaP-4					689:693	AaP-4	689:693	AaP-4	689:693	In addition, AaP-1, AaP-2, AaP-3, and AaP-4 were found to be glycoproteins, containing 21.75, 22.27, 25.62, and 18.31 % of carbohydrates, respectively.
24740695	4	3	located	found	700:704	arg2	AaP-1					664:668	AaP-1	664:668	AaP-1	664:668	In addition, AaP-1, AaP-2, AaP-3, and AaP-4 were found to be glycoproteins, containing 21.75, 22.27, 25.62, and 18.31 % of carbohydrates, respectively.
24740695	4	3	located	found	700:704	arg1	addition					654:661	addition	654:661	addition	654:661	In addition, AaP-1, AaP-2, AaP-3, and AaP-4 were found to be glycoproteins, containing 21.75, 22.27, 25.62, and 18.31 % of carbohydrates, respectively.
24740695	4	3	located	found	700:704	arg2	AaP-3					678:682	AaP-3	678:682	AaP-3	678:682	In addition, AaP-1, AaP-2, AaP-3, and AaP-4 were found to be glycoproteins, containing 21.75, 22.27, 25.62, and 18.31 % of carbohydrates, respectively.
24740695	4	3	located	found	700:704	arg2	AaP-2					671:675	AaP-2	671:675	AaP-2	671:675	In addition, AaP-1, AaP-2, AaP-3, and AaP-4 were found to be glycoproteins, containing 21.75, 22.27, 25.62, and 18.31 % of carbohydrates, respectively.
24740695	4	4	gly	glycoproteins	712:724	arg1	glycoproteins					712:724	glycoproteins	712:724	glycoproteins	712:724	In addition, AaP-1, AaP-2, AaP-3, and AaP-4 were found to be glycoproteins, containing 21.75, 22.27, 25.62, and 18.31 % of carbohydrates, respectively.
24740695	5	5	theme	AaPs	848:851	arg1	analysis					836:843	MALDI-TOF MS analysis	823:843	MALDI-TOF MS analysis	823:843	Peptide mapping and MALDI-TOF MS analysis of AaPs 1-4 showed that the structural differences between AaP-1 and AaP-2 and AaP-3 and AaPs-4 were mainly due to their glycan content.
24740695	5	5	theme	AaPs	848:851	arg1	mapping					811:817	Peptide mapping	803:817	Peptide mapping	803:817	Peptide mapping and MALDI-TOF MS analysis of AaPs 1-4 showed that the structural differences between AaP-1 and AaP-2 and AaP-3 and AaPs-4 were mainly due to their glycan content.
24740695	1	6	theme	cation-exchange	251:265	arg1	chromatographies					286:301	cation-exchange and gel filtration chromatographies	251:301	cation-exchange and gel filtration chromatographies	251:301	Four novel basic peroxidases, named AaP-1, AaP-2, AaP-3, and AaP-4, were purified from Asparagus acutifolius L. seeds by cation-exchange and gel filtration chromatographies.
24740695	0	7	theme	biotechnological	99:114	arg1	applications					116:127	biotechnological applications	99:127	biotechnological applications	99:127	Purification and characterization of novel cationic peroxidases from Asparagus acutifolius L. with biotechnological applications.
24740695	5	8	theme	glycan	966:971	arg1	content					973:979	their glycan content	960:979	their glycan content	960:979	Peptide mapping and MALDI-TOF MS analysis of AaPs 1-4 showed that the structural differences between AaP-1 and AaP-2 and AaP-3 and AaPs-4 were mainly due to their glycan content.
24740695	6	9	with	wastewaters	1071:1081	arg1	efficiency					1107:1116	a higher catalytic efficiency	1088:1116	a higher catalytic efficiency with respect to horseradish peroxidase	1088:1155	We also demonstrate that AaPs were able to remove phenolic compounds from olive oil mill wastewaters with a higher catalytic efficiency with respect to horseradish peroxidase, thus representing candidate enzymes for potential biotechnological applications in the environmental field.
24740695	6	10	theme	horseradish	1134:1144	arg1	peroxidase					1146:1155	horseradish peroxidase	1134:1155	horseradish peroxidase	1134:1155	We also demonstrate that AaPs were able to remove phenolic compounds from olive oil mill wastewaters with a higher catalytic efficiency with respect to horseradish peroxidase, thus representing candidate enzymes for potential biotechnological applications in the environmental field.
24740695	3	11	from	max	646:648	arg1	peroxidase					622:631	the peroxidase	618:631	the peroxidase from Glycine max	618:648	N-terminal sequences of AaPs 1-4 up to residue 20 showed a high percentage of identity with the peroxidase from Glycine max.
24740695	6	12	theme	phenolic	1032:1039	arg1	compounds					1041:1049	phenolic compounds	1032:1049	phenolic compounds	1032:1049	We also demonstrate that AaPs were able to remove phenolic compounds from olive oil mill wastewaters with a higher catalytic efficiency with respect to horseradish peroxidase, thus representing candidate enzymes for potential biotechnological applications in the environmental field.
24740695	2	13	theme	MALDI-TOF	384:392	arg1	MS					394:395	MALDI-TOF MS	384:395	MALDI-TOF MS	384:395	The four proteins showed a similar electrophoretic mobility of 46 kDa while, by MALDI-TOF MS, different Mr values of 42758.3, 41586.9, 42796.3, and 41595.5 were determined for AaP-1, AaP-2, AaP-3, and AaP-4, respectively.
24740695	2	14	theme	41586.9	430:436	arg1	values					411:416	different Mr values	398:416	different Mr values of 42758.3, 41586.9, 42796.3, and 41595.5	398:458	The four proteins showed a similar electrophoretic mobility of 46 kDa while, by MALDI-TOF MS, different Mr values of 42758.3, 41586.9, 42796.3, and 41595.5 were determined for AaP-1, AaP-2, AaP-3, and AaP-4, respectively.
24740695	6	15	from	applications	1225:1236	arg1	field					1259:1263	the environmental field	1241:1263	the environmental field	1241:1263	We also demonstrate that AaPs were able to remove phenolic compounds from olive oil mill wastewaters with a higher catalytic efficiency with respect to horseradish peroxidase, thus representing candidate enzymes for potential biotechnological applications in the environmental field.
24740695	5	16	theme	MALDI-TOF	823:831	arg1	analysis					836:843	MALDI-TOF MS analysis	823:843	MALDI-TOF MS analysis	823:843	Peptide mapping and MALDI-TOF MS analysis of AaPs 1-4 showed that the structural differences between AaP-1 and AaP-2 and AaP-3 and AaPs-4 were mainly due to their glycan content.
24740695	1	17	theme	gel	271:273	arg1	filtration					275:284	gel filtration	271:284	gel filtration	271:284	Four novel basic peroxidases, named AaP-1, AaP-2, AaP-3, and AaP-4, were purified from Asparagus acutifolius L. seeds by cation-exchange and gel filtration chromatographies.
24740695	3	18	theme	N-terminal	526:535	arg1	sequences					537:545	N-terminal sequences	526:545	N-terminal sequences of AaPs 1-4 up to residue 20	526:574	N-terminal sequences of AaPs 1-4 up to residue 20 showed a high percentage of identity with the peroxidase from Glycine max.
24740695	2	19	theme	42758.3	421:427	arg1	values					411:416	different Mr values	398:416	different Mr values of 42758.3, 41586.9, 42796.3, and 41595.5	398:458	The four proteins showed a similar electrophoretic mobility of 46 kDa while, by MALDI-TOF MS, different Mr values of 42758.3, 41586.9, 42796.3, and 41595.5 were determined for AaP-1, AaP-2, AaP-3, and AaP-4, respectively.
24740695	1	20	theme	filtration	275:284	arg1	chromatographies					286:301	cation-exchange and gel filtration chromatographies	251:301	cation-exchange and gel filtration chromatographies	251:301	Four novel basic peroxidases, named AaP-1, AaP-2, AaP-3, and AaP-4, were purified from Asparagus acutifolius L. seeds by cation-exchange and gel filtration chromatographies.
24740695	5	21	theme	MS	833:834	arg1	analysis					836:843	MALDI-TOF MS analysis	823:843	MALDI-TOF MS analysis	823:843	Peptide mapping and MALDI-TOF MS analysis of AaPs 1-4 showed that the structural differences between AaP-1 and AaP-2 and AaP-3 and AaPs-4 were mainly due to their glycan content.
24740695	6	22	theme	olive	1056:1060	arg1	oil					1062:1064	olive oil	1056:1064	olive oil mill wastewaters with a higher catalytic efficiency with respect to horseradish peroxidase	1056:1155	We also demonstrate that AaPs were able to remove phenolic compounds from olive oil mill wastewaters with a higher catalytic efficiency with respect to horseradish peroxidase, thus representing candidate enzymes for potential biotechnological applications in the environmental field.
24740695	3	23	theme	high	585:588	arg1	percentage					590:599	a high percentage	583:599	a high percentage of identity	583:611	N-terminal sequences of AaPs 1-4 up to residue 20 showed a high percentage of identity with the peroxidase from Glycine max.
24740695	2	24	theme	Mr	408:409	arg1	values					411:416	different Mr values	398:416	different Mr values of 42758.3, 41586.9, 42796.3, and 41595.5	398:458	The four proteins showed a similar electrophoretic mobility of 46 kDa while, by MALDI-TOF MS, different Mr values of 42758.3, 41586.9, 42796.3, and 41595.5 were determined for AaP-1, AaP-2, AaP-3, and AaP-4, respectively.
24740695	0	25	theme	cationic	43:50	arg1	peroxidases					52:62	novel cationic peroxidases	37:62	novel cationic peroxidases	37:62	Purification and characterization of novel cationic peroxidases from Asparagus acutifolius L. with biotechnological applications.
24740695	2	26	theme	electrophoretic	339:353	arg1	mobility					355:362	a similar electrophoretic mobility	329:362	a similar electrophoretic mobility of 46 kDa	329:372	The four proteins showed a similar electrophoretic mobility of 46 kDa while, by MALDI-TOF MS, different Mr values of 42758.3, 41586.9, 42796.3, and 41595.5 were determined for AaP-1, AaP-2, AaP-3, and AaP-4, respectively.
24740695	3	27	dep	residue	565:571	arg1	up					559:560	up	559:560	up	559:560	N-terminal sequences of AaPs 1-4 up to residue 20 showed a high percentage of identity with the peroxidase from Glycine max.
24740695	3	28	theme	AaPs	550:553	arg1	sequences					537:545	N-terminal sequences	526:545	N-terminal sequences of AaPs 1-4 up to residue 20	526:574	N-terminal sequences of AaPs 1-4 up to residue 20 showed a high percentage of identity with the peroxidase from Glycine max.
24740695	2	29	theme	different	398:406	arg1	values					411:416	different Mr values	398:416	different Mr values of 42758.3, 41586.9, 42796.3, and 41595.5	398:458	The four proteins showed a similar electrophoretic mobility of 46 kDa while, by MALDI-TOF MS, different Mr values of 42758.3, 41586.9, 42796.3, and 41595.5 were determined for AaP-1, AaP-2, AaP-3, and AaP-4, respectively.
24740695	0	30	theme	novel	37:41	arg1	peroxidases					52:62	novel cationic peroxidases	37:62	novel cationic peroxidases	37:62	Purification and characterization of novel cationic peroxidases from Asparagus acutifolius L. with biotechnological applications.
24740695	6	31	theme	biotechnological	1208:1223	arg1	applications					1225:1236	potential biotechnological applications	1198:1236	potential biotechnological applications in the environmental field	1198:1263	We also demonstrate that AaPs were able to remove phenolic compounds from olive oil mill wastewaters with a higher catalytic efficiency with respect to horseradish peroxidase, thus representing candidate enzymes for potential biotechnological applications in the environmental field.
24740695	5	32	theme	Peptide	803:809	arg1	mapping					811:817	Peptide mapping	803:817	Peptide mapping	803:817	Peptide mapping and MALDI-TOF MS analysis of AaPs 1-4 showed that the structural differences between AaP-1 and AaP-2 and AaP-3 and AaPs-4 were mainly due to their glycan content.
24740695	6	33	theme	environmental	1245:1257	arg1	field					1259:1263	the environmental field	1241:1263	the environmental field	1241:1263	We also demonstrate that AaPs were able to remove phenolic compounds from olive oil mill wastewaters with a higher catalytic efficiency with respect to horseradish peroxidase, thus representing candidate enzymes for potential biotechnological applications in the environmental field.
24740695	2	34	theme	42796.3	439:445	arg1	values					411:416	different Mr values	398:416	different Mr values of 42758.3, 41586.9, 42796.3, and 41595.5	398:458	The four proteins showed a similar electrophoretic mobility of 46 kDa while, by MALDI-TOF MS, different Mr values of 42758.3, 41586.9, 42796.3, and 41595.5 were determined for AaP-1, AaP-2, AaP-3, and AaP-4, respectively.
24740695	4	35	theme	carbohydrates	774:786	arg1	carbohydrates					774:786	carbohydrates	774:786	carbohydrates	774:786	In addition, AaP-1, AaP-2, AaP-3, and AaP-4 were found to be glycoproteins, containing 21.75, 22.27, 25.62, and 18.31 % of carbohydrates, respectively.
24740695	4	35	theme	carbohydrates	774:786	arg1	%					769:769	21.75, 22.27, 25.62, and 18.31 %	738:769	21.75, 22.27, 25.62, and 18.31 % of carbohydrates	738:786	In addition, AaP-1, AaP-2, AaP-3, and AaP-4 were found to be glycoproteins, containing 21.75, 22.27, 25.62, and 18.31 % of carbohydrates, respectively.
24740695	0	36	from	Asparagus	69:77	arg1	characterization					17:32	characterization	17:32	characterization	17:32	Purification and characterization of novel cationic peroxidases from Asparagus acutifolius L. with biotechnological applications.
24740695	0	36	from	Asparagus	69:77	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification and characterization of novel cationic peroxidases from Asparagus acutifolius L. with biotechnological applications.
24740695	6	37	theme	higher	1090:1095	arg1	efficiency					1107:1116	a higher catalytic efficiency	1088:1116	a higher catalytic efficiency with respect to horseradish peroxidase	1088:1155	We also demonstrate that AaPs were able to remove phenolic compounds from olive oil mill wastewaters with a higher catalytic efficiency with respect to horseradish peroxidase, thus representing candidate enzymes for potential biotechnological applications in the environmental field.
24740695	0	38	with	characterization	17:32	arg1	applications					116:127	biotechnological applications	99:127	biotechnological applications	99:127	Purification and characterization of novel cationic peroxidases from Asparagus acutifolius L. with biotechnological applications.
24740695	1	39	theme	novel	135:139	arg1	peroxidases					147:157	Four novel basic peroxidases	130:157	Four novel basic peroxidases	130:157	Four novel basic peroxidases, named AaP-1, AaP-2, AaP-3, and AaP-4, were purified from Asparagus acutifolius L. seeds by cation-exchange and gel filtration chromatographies.
24740695	1	39	theme	novel	135:139	arg1	AaP-1					166:170	AaP-1	166:170	AaP-1	166:170	Four novel basic peroxidases, named AaP-1, AaP-2, AaP-3, and AaP-4, were purified from Asparagus acutifolius L. seeds by cation-exchange and gel filtration chromatographies.
24740695	1	39	theme	novel	135:139	arg1	AaP-3					180:184	AaP-3	180:184	AaP-3	180:184	Four novel basic peroxidases, named AaP-1, AaP-2, AaP-3, and AaP-4, were purified from Asparagus acutifolius L. seeds by cation-exchange and gel filtration chromatographies.
24740695	1	39	theme	novel	135:139	arg1	AaP-4					191:195	AaP-4	191:195	AaP-4	191:195	Four novel basic peroxidases, named AaP-1, AaP-2, AaP-3, and AaP-4, were purified from Asparagus acutifolius L. seeds by cation-exchange and gel filtration chromatographies.
24740695	1	39	theme	novel	135:139	arg1	AaP-2					173:177	AaP-2	173:177	AaP-2	173:177	Four novel basic peroxidases, named AaP-1, AaP-2, AaP-3, and AaP-4, were purified from Asparagus acutifolius L. seeds by cation-exchange and gel filtration chromatographies.
24740695	0	40	theme	peroxidases	52:62	arg1	characterization					17:32	characterization	17:32	characterization	17:32	Purification and characterization of novel cationic peroxidases from Asparagus acutifolius L. with biotechnological applications.
24740695	0	40	theme	peroxidases	52:62	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification and characterization of novel cationic peroxidases from Asparagus acutifolius L. with biotechnological applications.
24740695	6	41	theme	catalytic	1097:1105	arg1	efficiency					1107:1116	a higher catalytic efficiency	1088:1116	a higher catalytic efficiency with respect to horseradish peroxidase	1088:1155	We also demonstrate that AaPs were able to remove phenolic compounds from olive oil mill wastewaters with a higher catalytic efficiency with respect to horseradish peroxidase, thus representing candidate enzymes for potential biotechnological applications in the environmental field.
24740695	1	42	theme	basic	141:145	arg1	peroxidases					147:157	Four novel basic peroxidases	130:157	Four novel basic peroxidases	130:157	Four novel basic peroxidases, named AaP-1, AaP-2, AaP-3, and AaP-4, were purified from Asparagus acutifolius L. seeds by cation-exchange and gel filtration chromatographies.
24740695	1	42	theme	basic	141:145	arg1	AaP-1					166:170	AaP-1	166:170	AaP-1	166:170	Four novel basic peroxidases, named AaP-1, AaP-2, AaP-3, and AaP-4, were purified from Asparagus acutifolius L. seeds by cation-exchange and gel filtration chromatographies.
24740695	1	42	theme	basic	141:145	arg1	AaP-3					180:184	AaP-3	180:184	AaP-3	180:184	Four novel basic peroxidases, named AaP-1, AaP-2, AaP-3, and AaP-4, were purified from Asparagus acutifolius L. seeds by cation-exchange and gel filtration chromatographies.
24740695	1	42	theme	basic	141:145	arg1	AaP-4					191:195	AaP-4	191:195	AaP-4	191:195	Four novel basic peroxidases, named AaP-1, AaP-2, AaP-3, and AaP-4, were purified from Asparagus acutifolius L. seeds by cation-exchange and gel filtration chromatographies.
24740695	1	42	theme	basic	141:145	arg1	AaP-2					173:177	AaP-2	173:177	AaP-2	173:177	Four novel basic peroxidases, named AaP-1, AaP-2, AaP-3, and AaP-4, were purified from Asparagus acutifolius L. seeds by cation-exchange and gel filtration chromatographies.
24740695	0	43	with	Purification	0:11	arg1	applications					116:127	biotechnological applications	99:127	biotechnological applications	99:127	Purification and characterization of novel cationic peroxidases from Asparagus acutifolius L. with biotechnological applications.
24740695	2	44	theme	similar	331:337	arg1	mobility					355:362	a similar electrophoretic mobility	329:362	a similar electrophoretic mobility of 46 kDa	329:372	The four proteins showed a similar electrophoretic mobility of 46 kDa while, by MALDI-TOF MS, different Mr values of 42758.3, 41586.9, 42796.3, and 41595.5 were determined for AaP-1, AaP-2, AaP-3, and AaP-4, respectively.
24740695	6	45	theme	candidate	1176:1184	arg1	enzymes					1186:1192	candidate enzymes	1176:1192	candidate enzymes for potential biotechnological applications in the environmental field	1176:1263	We also demonstrate that AaPs were able to remove phenolic compounds from olive oil mill wastewaters with a higher catalytic efficiency with respect to horseradish peroxidase, thus representing candidate enzymes for potential biotechnological applications in the environmental field.
24740695	5	46	theme	structural	873:882	arg1	differences					884:894	the structural differences	869:894	the structural differences between AaP-1 and AaP-2 and AaP-3 and AaPs-4	869:939	Peptide mapping and MALDI-TOF MS analysis of AaPs 1-4 showed that the structural differences between AaP-1 and AaP-2 and AaP-3 and AaPs-4 were mainly due to their glycan content.
24740695	5	46	theme	structural	873:882	arg1	due					953:955	due	953:955	due	953:955	Peptide mapping and MALDI-TOF MS analysis of AaPs 1-4 showed that the structural differences between AaP-1 and AaP-2 and AaP-3 and AaPs-4 were mainly due to their glycan content.
24740695	1	47	theme	Asparagus	217:225	arg1	seeds					242:246	Asparagus acutifolius L. seeds	217:246	Asparagus acutifolius L. seeds	217:246	Four novel basic peroxidases, named AaP-1, AaP-2, AaP-3, and AaP-4, were purified from Asparagus acutifolius L. seeds by cation-exchange and gel filtration chromatographies.
24740695	3	48	theme	identity	604:611	arg1	percentage					590:599	a high percentage	583:599	a high percentage of identity	583:611	N-terminal sequences of AaPs 1-4 up to residue 20 showed a high percentage of identity with the peroxidase from Glycine max.
24740695	6	49	theme	mill	1066:1069	arg1	wastewaters					1071:1081	olive oil mill wastewaters	1056:1081	olive oil mill wastewaters with a higher catalytic efficiency with respect to horseradish peroxidase	1056:1155	We also demonstrate that AaPs were able to remove phenolic compounds from olive oil mill wastewaters with a higher catalytic efficiency with respect to horseradish peroxidase, thus representing candidate enzymes for potential biotechnological applications in the environmental field.
24740695	1	50	theme	named	160:164	arg1	peroxidases					147:157	Four novel basic peroxidases	130:157	Four novel basic peroxidases	130:157	Four novel basic peroxidases, named AaP-1, AaP-2, AaP-3, and AaP-4, were purified from Asparagus acutifolius L. seeds by cation-exchange and gel filtration chromatographies.
24740695	1	50	theme	named	160:164	arg1	AaP-1					166:170	AaP-1	166:170	AaP-1	166:170	Four novel basic peroxidases, named AaP-1, AaP-2, AaP-3, and AaP-4, were purified from Asparagus acutifolius L. seeds by cation-exchange and gel filtration chromatographies.
24740695	1	51	theme	acutifolius	227:237	arg1	seeds					242:246	Asparagus acutifolius L. seeds	217:246	Asparagus acutifolius L. seeds	217:246	Four novel basic peroxidases, named AaP-1, AaP-2, AaP-3, and AaP-4, were purified from Asparagus acutifolius L. seeds by cation-exchange and gel filtration chromatographies.
24740695	3	52	theme	Glycine	638:644	arg1	max					646:648	Glycine max	638:648	Glycine max	638:648	N-terminal sequences of AaPs 1-4 up to residue 20 showed a high percentage of identity with the peroxidase from Glycine max.
24740695	6	53	theme	oil	1062:1064	arg1	wastewaters					1071:1081	olive oil mill wastewaters	1056:1081	olive oil mill wastewaters with a higher catalytic efficiency with respect to horseradish peroxidase	1056:1155	We also demonstrate that AaPs were able to remove phenolic compounds from olive oil mill wastewaters with a higher catalytic efficiency with respect to horseradish peroxidase, thus representing candidate enzymes for potential biotechnological applications in the environmental field.
24740695	2	54	theme	kDa	370:372	arg1	mobility					355:362	a similar electrophoretic mobility	329:362	a similar electrophoretic mobility of 46 kDa	329:372	The four proteins showed a similar electrophoretic mobility of 46 kDa while, by MALDI-TOF MS, different Mr values of 42758.3, 41586.9, 42796.3, and 41595.5 were determined for AaP-1, AaP-2, AaP-3, and AaP-4, respectively.
24740695	1	55	theme	L.	239:240	arg1	seeds					242:246	Asparagus acutifolius L. seeds	217:246	Asparagus acutifolius L. seeds	217:246	Four novel basic peroxidases, named AaP-1, AaP-2, AaP-3, and AaP-4, were purified from Asparagus acutifolius L. seeds by cation-exchange and gel filtration chromatographies.
27809330	7	0	dep	CONCLUSIONS	1024:1034	arg1	composites					1049:1058	The proposed composites	1036:1058	The proposed composites	1036:1058	CONCLUSIONS The proposed composites, named Poly-paper, appear to warrant further investigation as highly sustainable packaging.
27809330	1	1	theme	sustainability	137:150	arg1	issues					152:157	environmental sustainability issues	123:157	environmental sustainability issues	123:157	BACKGROUND Until now, environmental sustainability issues are almost entirely unsolved for packaging materials.
27809330	0	2	theme	recycled	59:66	arg1	paper					68:72	recycled paper	59:72	recycled paper	59:72	Poly-paper: a sustainable material for packaging, based on recycled paper and recyclable with paper.
27809330	5	3	theme	room	700:703	arg1	temperature					705:715	room temperature	700:715	room temperature	700:715	Solubility tests performed at room temperature and 45°C showed different behavior depending on the water-resistant film applied on the composite (50% cellulose fiber content).
27809330	6	4	theme	uncoated	865:872	arg1	composite					874:882	the uncoated composite	861:882	the uncoated composite	861:882	In particular, the uncoated composite showed complete solubility after 2 hours, whereas at the same time point, no solubility occurred when a non-water-soluble varnish was used.
27809330	6	5	theme	non-water-soluble	988:1004	arg1	varnish					1006:1012	a non-water-soluble varnish	986:1012	a non-water-soluble varnish	986:1012	In particular, the uncoated composite showed complete solubility after 2 hours, whereas at the same time point, no solubility occurred when a non-water-soluble varnish was used.
27809330	6	6	theme	time	946:949	arg1	point					951:955	the same time point	937:955	the same time point	937:955	In particular, the uncoated composite showed complete solubility after 2 hours, whereas at the same time point, no solubility occurred when a non-water-soluble varnish was used.
27809330	4	7	from	improvement	540:550	arg1	stiffness					565:573	composite stiffness	555:573	composite stiffness	555:573	RESULTS Tensile mechanical tests exhibited an improvement in composite stiffness when the reinforcement content was increased together with a decrease in composite elongation.
27809330	2	8	theme	recycling	267:275	arg1	channel					277:283	a single recycling channel	258:283	a single recycling channel	258:283	With the final aim of finding materials with a single recycling channel, cellulose fiber/poly(vinyl)alcohol composites were investigated.
27809330	4	9	from	decrease	636:643	arg1	elongation					658:667	composite elongation	648:667	composite elongation	648:667	RESULTS Tensile mechanical tests exhibited an improvement in composite stiffness when the reinforcement content was increased together with a decrease in composite elongation.
27809330	6	10	theme	same	941:944	arg1	point					951:955	the same time point	937:955	the same time point	937:955	In particular, the uncoated composite showed complete solubility after 2 hours, whereas at the same time point, no solubility occurred when a non-water-soluble varnish was used.
27809330	5	11	theme	50	816:817	arg1	%					818:818	%	818:818	%	818:818	Solubility tests performed at room temperature and 45°C showed different behavior depending on the water-resistant film applied on the composite (50% cellulose fiber content).
27809330	4	12	theme	reinforcement	584:596	arg1	content					598:604	the reinforcement content	580:604	the reinforcement content	580:604	RESULTS Tensile mechanical tests exhibited an improvement in composite stiffness when the reinforcement content was increased together with a decrease in composite elongation.
27809330	2	13	theme	single	260:265	arg1	channel					277:283	a single recycling channel	258:283	a single recycling channel	258:283	With the final aim of finding materials with a single recycling channel, cellulose fiber/poly(vinyl)alcohol composites were investigated.
27809330	3	14	theme	different	424:432	arg1	content					450:456	different cellulose fiber content	424:456	different cellulose fiber content (30%, 50% and 70% w/w)	424:479	METHODS After extrusion and injection molding, samples of composite with different cellulose fiber content (30%, 50% and 70% w/w) were tested.
27809330	6	15	used	used	1018:1021	arg2	varnish					1006:1012	a non-water-soluble varnish	986:1012	a non-water-soluble varnish	986:1012	In particular, the uncoated composite showed complete solubility after 2 hours, whereas at the same time point, no solubility occurred when a non-water-soluble varnish was used.
27809330	1	16	dep	BACKGROUND	101:110	arg1	now					118:120	now	118:120	now	118:120	BACKGROUND Until now, environmental sustainability issues are almost entirely unsolved for packaging materials.
27809330	3	17	theme	cellulose	434:442	arg1	content					450:456	different cellulose fiber content	424:456	different cellulose fiber content (30%, 50% and 70% w/w)	424:479	METHODS After extrusion and injection molding, samples of composite with different cellulose fiber content (30%, 50% and 70% w/w) were tested.
27809330	4	18	dep	RESULTS	494:500	arg1	exhibited					527:535	exhibited	527:535	exhibited an improvement in composite stiffness when the reinforcement content was increased together with a decrease in composite elongation	527:667	RESULTS Tensile mechanical tests exhibited an improvement in composite stiffness when the reinforcement content was increased together with a decrease in composite elongation.
27809330	7	19	theme	proposed	1040:1047	arg1	composites					1049:1058	The proposed composites	1036:1058	The proposed composites	1036:1058	CONCLUSIONS The proposed composites, named Poly-paper, appear to warrant further investigation as highly sustainable packaging.
27809330	3	20	theme	composite	409:417	arg1	samples					398:404	samples	398:404	samples of composite with different cellulose fiber content (30%, 50% and 70% w/w)	398:479	METHODS After extrusion and injection molding, samples of composite with different cellulose fiber content (30%, 50% and 70% w/w) were tested.
27809330	3	21	theme	injection	379:387	arg1	molding					389:395	injection molding	379:395	injection molding	379:395	METHODS After extrusion and injection molding, samples of composite with different cellulose fiber content (30%, 50% and 70% w/w) were tested.
27809330	3	21	theme	injection	379:387	arg1	METHODS					351:357	METHODS	351:357	METHODS	351:357	METHODS After extrusion and injection molding, samples of composite with different cellulose fiber content (30%, 50% and 70% w/w) were tested.
27809330	4	22	theme	Tensile	502:508	arg1	tests					521:525	Tensile mechanical tests	502:525	Tensile mechanical tests	502:525	RESULTS Tensile mechanical tests exhibited an improvement in composite stiffness when the reinforcement content was increased together with a decrease in composite elongation.
27809330	0	23	with	recyclable	78:87	arg1	paper					94:98	paper	94:98	paper	94:98	Poly-paper: a sustainable material for packaging, based on recycled paper and recyclable with paper.
27809330	2	24	theme	fiber/poly	296:305	arg1	composites					321:330	cellulose fiber/poly(vinyl)alcohol composites	286:330	cellulose fiber/poly(vinyl)alcohol composites	286:330	With the final aim of finding materials with a single recycling channel, cellulose fiber/poly(vinyl)alcohol composites were investigated.
27809330	0	25	theme	sustainable	14:24	arg1	material					26:33	a sustainable material	12:33	Poly-paper: a sustainable material for packaging, based on recycled paper and recyclable with paper.	0:99	Poly-paper: a sustainable material for packaging, based on recycled paper and recyclable with paper.
27809330	5	26	theme	Solubility	670:679	arg1	tests					681:685	Solubility tests	670:685	Solubility tests performed at room temperature and 45°C	670:724	Solubility tests performed at room temperature and 45°C showed different behavior depending on the water-resistant film applied on the composite (50% cellulose fiber content).
27809330	2	27	theme	cellulose	286:294	arg1	composites					321:330	cellulose fiber/poly(vinyl)alcohol composites	286:330	cellulose fiber/poly(vinyl)alcohol composites	286:330	With the final aim of finding materials with a single recycling channel, cellulose fiber/poly(vinyl)alcohol composites were investigated.
27809330	7	28	theme	sustainable	1129:1139	arg1	packaging					1141:1149	highly sustainable packaging	1122:1149	highly sustainable packaging	1122:1149	CONCLUSIONS The proposed composites, named Poly-paper, appear to warrant further investigation as highly sustainable packaging.
27809330	1	29	theme	packaging	192:200	arg1	materials					202:210	packaging materials	192:210	packaging materials	192:210	BACKGROUND Until now, environmental sustainability issues are almost entirely unsolved for packaging materials.
27809330	4	30	theme	mechanical	510:519	arg1	tests					521:525	Tensile mechanical tests	502:525	Tensile mechanical tests	502:525	RESULTS Tensile mechanical tests exhibited an improvement in composite stiffness when the reinforcement content was increased together with a decrease in composite elongation.
27809330	5	31	theme	%	818:818	arg1	content					836:842	50% cellulose fiber content	816:842	50% cellulose fiber content	816:842	Solubility tests performed at room temperature and 45°C showed different behavior depending on the water-resistant film applied on the composite (50% cellulose fiber content).
27809330	5	31	theme	%	818:818	arg1	composite					805:813	the composite	801:813	the composite (50% cellulose fiber content)	801:843	Solubility tests performed at room temperature and 45°C showed different behavior depending on the water-resistant film applied on the composite (50% cellulose fiber content).
27809330	3	32	theme	fiber	444:448	arg1	content					450:456	different cellulose fiber content	424:456	different cellulose fiber content (30%, 50% and 70% w/w)	424:479	METHODS After extrusion and injection molding, samples of composite with different cellulose fiber content (30%, 50% and 70% w/w) were tested.
27809330	5	33	theme	fiber	830:834	arg1	content					836:842	50% cellulose fiber content	816:842	50% cellulose fiber content	816:842	Solubility tests performed at room temperature and 45°C showed different behavior depending on the water-resistant film applied on the composite (50% cellulose fiber content).
27809330	5	33	theme	fiber	830:834	arg1	composite					805:813	the composite	801:813	the composite (50% cellulose fiber content)	801:843	Solubility tests performed at room temperature and 45°C showed different behavior depending on the water-resistant film applied on the composite (50% cellulose fiber content).
27809330	2	34	theme	final	222:226	arg1	aim					228:230	the final aim	218:230	the final aim of finding materials with a single recycling channel	218:283	With the final aim of finding materials with a single recycling channel, cellulose fiber/poly(vinyl)alcohol composites were investigated.
27809330	5	35	theme	cellulose	820:828	arg1	content					836:842	50% cellulose fiber content	816:842	50% cellulose fiber content	816:842	Solubility tests performed at room temperature and 45°C showed different behavior depending on the water-resistant film applied on the composite (50% cellulose fiber content).
27809330	5	35	theme	cellulose	820:828	arg1	composite					805:813	the composite	801:813	the composite (50% cellulose fiber content)	801:843	Solubility tests performed at room temperature and 45°C showed different behavior depending on the water-resistant film applied on the composite (50% cellulose fiber content).
27809330	5	36	theme	water-resistant	769:783	arg1	film					785:788	the water-resistant film	765:788	the water-resistant film applied on the composite (50% cellulose fiber content)	765:843	Solubility tests performed at room temperature and 45°C showed different behavior depending on the water-resistant film applied on the composite (50% cellulose fiber content).
27809330	3	37	with	composite	409:417	arg1	content					450:456	different cellulose fiber content	424:456	different cellulose fiber content (30%, 50% and 70% w/w)	424:479	METHODS After extrusion and injection molding, samples of composite with different cellulose fiber content (30%, 50% and 70% w/w) were tested.
27809330	7	38	theme	further	1097:1103	arg1	investigation					1105:1117	further investigation	1097:1117	further investigation	1097:1117	CONCLUSIONS The proposed composites, named Poly-paper, appear to warrant further investigation as highly sustainable packaging.
27809330	2	39	theme	alcohol	313:319	arg1	composites					321:330	cellulose fiber/poly(vinyl)alcohol composites	286:330	cellulose fiber/poly(vinyl)alcohol composites	286:330	With the final aim of finding materials with a single recycling channel, cellulose fiber/poly(vinyl)alcohol composites were investigated.
27809330	2	40	with	materials	243:251	arg1	channel					277:283	a single recycling channel	258:283	a single recycling channel	258:283	With the final aim of finding materials with a single recycling channel, cellulose fiber/poly(vinyl)alcohol composites were investigated.
27809330	3	41	dep	%	474:474	arg1	w/w					476:478	w/w	476:478	70% w/w	472:478	METHODS After extrusion and injection molding, samples of composite with different cellulose fiber content (30%, 50% and 70% w/w) were tested.
27809330	3	42	dep	METHODS	351:357	arg1	extrusion					365:373	extrusion	365:373	extrusion	365:373	METHODS After extrusion and injection molding, samples of composite with different cellulose fiber content (30%, 50% and 70% w/w) were tested.
27809330	3	42	dep	METHODS	351:357	arg1	molding					389:395	injection molding	379:395	injection molding	379:395	METHODS After extrusion and injection molding, samples of composite with different cellulose fiber content (30%, 50% and 70% w/w) were tested.
27809330	3	42	dep	METHODS	351:357	arg1	METHODS					351:357	METHODS	351:357	METHODS	351:357	METHODS After extrusion and injection molding, samples of composite with different cellulose fiber content (30%, 50% and 70% w/w) were tested.
27809330	3	43	dep	content	450:456	arg1	%					461:461	30%	459:461	30%	459:461	METHODS After extrusion and injection molding, samples of composite with different cellulose fiber content (30%, 50% and 70% w/w) were tested.
27809330	3	43	dep	content	450:456	arg1	%					466:466	50%	464:466	50%	464:466	METHODS After extrusion and injection molding, samples of composite with different cellulose fiber content (30%, 50% and 70% w/w) were tested.
27809330	3	43	dep	content	450:456	arg1	%					474:474	70%	472:474	70% w/w	472:478	METHODS After extrusion and injection molding, samples of composite with different cellulose fiber content (30%, 50% and 70% w/w) were tested.
27809330	2	44	theme	vinyl	307:311	arg1	composites					321:330	cellulose fiber/poly(vinyl)alcohol composites	286:330	cellulose fiber/poly(vinyl)alcohol composites	286:330	With the final aim of finding materials with a single recycling channel, cellulose fiber/poly(vinyl)alcohol composites were investigated.
27809330	4	45	theme	composite	555:563	arg1	stiffness					565:573	composite stiffness	555:573	composite stiffness	555:573	RESULTS Tensile mechanical tests exhibited an improvement in composite stiffness when the reinforcement content was increased together with a decrease in composite elongation.
27809330	4	46	theme	composite	648:656	arg1	elongation					658:667	composite elongation	648:667	composite elongation	648:667	RESULTS Tensile mechanical tests exhibited an improvement in composite stiffness when the reinforcement content was increased together with a decrease in composite elongation.
27809330	5	47	theme	different	733:741	arg1	behavior					743:750	different behavior	733:750	different behavior	733:750	Solubility tests performed at room temperature and 45°C showed different behavior depending on the water-resistant film applied on the composite (50% cellulose fiber content).
27809330	6	48	theme	complete	891:898	arg1	solubility					900:909	complete solubility	891:909	complete solubility	891:909	In particular, the uncoated composite showed complete solubility after 2 hours, whereas at the same time point, no solubility occurred when a non-water-soluble varnish was used.
27809330	0	49	dep	Poly-paper	0:9	arg1	material					26:33	a sustainable material	12:33	Poly-paper: a sustainable material for packaging, based on recycled paper and recyclable with paper.	0:99	Poly-paper: a sustainable material for packaging, based on recycled paper and recyclable with paper.
27809330	1	50	theme	environmental	123:135	arg1	issues					152:157	environmental sustainability issues	123:157	environmental sustainability issues	123:157	BACKGROUND Until now, environmental sustainability issues are almost entirely unsolved for packaging materials.
26652767	3	0	theme	following	914:922	arg1	results					924:930	the following results	910:930	the following results	910:930	For neutral pectic polysaccharides, the following results were achieved: BP (enzymatic-assisted extraction, 24 h, 85.9%), PP (nitric acid-assisted extraction, 6 h, 82.2%), and SBP (enzymatic assisted extraction, 48 h, 97.5%; and nitric acid-assisted extraction, 4 h, 83.2%).
26652767	1	1	theme	sugar	453:457	arg1	residues					372:379	four agro-industrial residues	351:379	four agro-industrial residues	351:379	The influence of different extraction methodologies was assessed on the composition of both neutral (arabinose, rhamnose, galactose) and acidic (galacturonic acid) pectic polysaccharides obtained from four agro-industrial residues, namely, berry pomace (BP), onion hulls (OH), pressed pumpkin (PP), and sugar beet pulp (SBP).
26652767	1	1	theme	sugar	453:457	arg1	pulp					464:467	sugar beet pulp	453:467	sugar beet pulp	453:467	The influence of different extraction methodologies was assessed on the composition of both neutral (arabinose, rhamnose, galactose) and acidic (galacturonic acid) pectic polysaccharides obtained from four agro-industrial residues, namely, berry pomace (BP), onion hulls (OH), pressed pumpkin (PP), and sugar beet pulp (SBP).
26652767	1	2	theme	neutral	242:248	arg1	galactose					272:280	both neutral (arabinose, rhamnose, galactose) and acidic (galacturonic acid) pectic polysaccharides	237:335	galactose	272:280	The influence of different extraction methodologies was assessed on the composition of both neutral (arabinose, rhamnose, galactose) and acidic (galacturonic acid) pectic polysaccharides obtained from four agro-industrial residues, namely, berry pomace (BP), onion hulls (OH), pressed pumpkin (PP), and sugar beet pulp (SBP).
26652767	3	3	theme	neutral	878:884	arg1	polysaccharides					893:907	neutral pectic polysaccharides	878:907	neutral pectic polysaccharides	878:907	For neutral pectic polysaccharides, the following results were achieved: BP (enzymatic-assisted extraction, 24 h, 85.9%), PP (nitric acid-assisted extraction, 6 h, 82.2%), and SBP (enzymatic assisted extraction, 48 h, 97.5%; and nitric acid-assisted extraction, 4 h, 83.2%).
26652767	1	4	theme	pectic	314:319	arg1	acid					308:311	galacturonic acid	295:311	galacturonic acid	295:311	The influence of different extraction methodologies was assessed on the composition of both neutral (arabinose, rhamnose, galactose) and acidic (galacturonic acid) pectic polysaccharides obtained from four agro-industrial residues, namely, berry pomace (BP), onion hulls (OH), pressed pumpkin (PP), and sugar beet pulp (SBP).
26652767	1	4	theme	pectic	314:319	arg1	polysaccharides					321:335	both neutral (arabinose, rhamnose, galactose) and acidic (galacturonic acid) pectic polysaccharides	237:335	polysaccharides	321:335	The influence of different extraction methodologies was assessed on the composition of both neutral (arabinose, rhamnose, galactose) and acidic (galacturonic acid) pectic polysaccharides obtained from four agro-industrial residues, namely, berry pomace (BP), onion hulls (OH), pressed pumpkin (PP), and sugar beet pulp (SBP).
26652767	2	5	theme	nitric	557:562	arg1	extraction					578:587	nitric acid-assisted extraction	557:587	nitric acid-assisted extraction	557:587	For acidic pectic polysaccharides, the extraction efficiency was obtained as BP (nitric acid-assisted extraction, 2 h, 62.9%), PP (enzymatic-assisted extraction, 12 h, 75.0%), SBP (enzymatic-assisted extraction, 48 h, 89.8%; and nitric acid-assisted extraction, 4 h, 76.5%), and OH (sodium hexametaphosphate-assisted extraction, 0.5 h, 100%; and ammonium oxalate-assisted extraction, 0.5 h, 100%).
26652767	2	5	theme	nitric	557:562	arg1	h					592:592	2 h	590:592	2 h	590:592	For acidic pectic polysaccharides, the extraction efficiency was obtained as BP (nitric acid-assisted extraction, 2 h, 62.9%), PP (enzymatic-assisted extraction, 12 h, 75.0%), SBP (enzymatic-assisted extraction, 48 h, 89.8%; and nitric acid-assisted extraction, 4 h, 76.5%), and OH (sodium hexametaphosphate-assisted extraction, 0.5 h, 100%; and ammonium oxalate-assisted extraction, 0.5 h, 100%).
26652767	2	5	theme	nitric	557:562	arg1	%					599:599	62.9%	595:599	62.9%	595:599	For acidic pectic polysaccharides, the extraction efficiency was obtained as BP (nitric acid-assisted extraction, 2 h, 62.9%), PP (enzymatic-assisted extraction, 12 h, 75.0%), SBP (enzymatic-assisted extraction, 48 h, 89.8%; and nitric acid-assisted extraction, 4 h, 76.5%), and OH (sodium hexametaphosphate-assisted extraction, 0.5 h, 100%; and ammonium oxalate-assisted extraction, 0.5 h, 100%).
26652767	2	6	theme	nitric	705:710	arg1	extraction					726:735	nitric acid-assisted extraction	705:735	nitric acid-assisted extraction	705:735	For acidic pectic polysaccharides, the extraction efficiency was obtained as BP (nitric acid-assisted extraction, 2 h, 62.9%), PP (enzymatic-assisted extraction, 12 h, 75.0%), SBP (enzymatic-assisted extraction, 48 h, 89.8%; and nitric acid-assisted extraction, 4 h, 76.5%), and OH (sodium hexametaphosphate-assisted extraction, 0.5 h, 100%; and ammonium oxalate-assisted extraction, 0.5 h, 100%).
26652767	1	7	theme	polysaccharides	321:335	arg1	composition					222:232	the composition	218:232	the composition of both neutral (arabinose, rhamnose, galactose) and acidic (galacturonic acid) pectic polysaccharides obtained from four agro-industrial residues, namely, berry pomace (BP), onion hulls (OH), pressed pumpkin (PP), and sugar beet pulp (SBP)	218:473	The influence of different extraction methodologies was assessed on the composition of both neutral (arabinose, rhamnose, galactose) and acidic (galacturonic acid) pectic polysaccharides obtained from four agro-industrial residues, namely, berry pomace (BP), onion hulls (OH), pressed pumpkin (PP), and sugar beet pulp (SBP).
26652767	3	8	theme	assisted	1065:1072	arg1	extraction					1074:1083	enzymatic assisted extraction	1055:1083	enzymatic assisted extraction	1055:1083	For neutral pectic polysaccharides, the following results were achieved: BP (enzymatic-assisted extraction, 24 h, 85.9%), PP (nitric acid-assisted extraction, 6 h, 82.2%), and SBP (enzymatic assisted extraction, 48 h, 97.5%; and nitric acid-assisted extraction, 4 h, 83.2%).
26652767	2	9	theme	enzymatic-assisted	657:674	arg1	extraction					676:685	enzymatic-assisted extraction	657:685	enzymatic-assisted extraction	657:685	For acidic pectic polysaccharides, the extraction efficiency was obtained as BP (nitric acid-assisted extraction, 2 h, 62.9%), PP (enzymatic-assisted extraction, 12 h, 75.0%), SBP (enzymatic-assisted extraction, 48 h, 89.8%; and nitric acid-assisted extraction, 4 h, 76.5%), and OH (sodium hexametaphosphate-assisted extraction, 0.5 h, 100%; and ammonium oxalate-assisted extraction, 0.5 h, 100%).
26652767	0	10	from	Residues	140:147	arg1	Extract					103:109	the Crude Pectic Extract	86:109	the Crude Pectic Extract from Various Agro-Industrial Residues	86:147	Effect of Extraction Conditions on the Saccharide (Neutral and Acidic) Composition of the Crude Pectic Extract from Various Agro-Industrial Residues.
26652767	0	10	from	Residues	140:147	arg1	Composition					71:81	the Saccharide (Neutral and Acidic) Composition	35:81	the Saccharide (Neutral and Acidic) Composition of the Crude Pectic Extract from Various Agro-Industrial Residues	35:147	Effect of Extraction Conditions on the Saccharide (Neutral and Acidic) Composition of the Crude Pectic Extract from Various Agro-Industrial Residues.
26652767	3	11	theme	enzymatic-assisted	951:968	arg1	extraction					970:979	enzymatic-assisted extraction	951:979	enzymatic-assisted extraction	951:979	For neutral pectic polysaccharides, the following results were achieved: BP (enzymatic-assisted extraction, 24 h, 85.9%), PP (nitric acid-assisted extraction, 6 h, 82.2%), and SBP (enzymatic assisted extraction, 48 h, 97.5%; and nitric acid-assisted extraction, 4 h, 83.2%).
26652767	3	11	theme	enzymatic-assisted	951:968	arg1	h					985:985	24 h	982:985	24 h	982:985	For neutral pectic polysaccharides, the following results were achieved: BP (enzymatic-assisted extraction, 24 h, 85.9%), PP (nitric acid-assisted extraction, 6 h, 82.2%), and SBP (enzymatic assisted extraction, 48 h, 97.5%; and nitric acid-assisted extraction, 4 h, 83.2%).
26652767	3	11	theme	enzymatic-assisted	951:968	arg1	%					992:992	85.9%	988:992	85.9%	988:992	For neutral pectic polysaccharides, the following results were achieved: BP (enzymatic-assisted extraction, 24 h, 85.9%), PP (nitric acid-assisted extraction, 6 h, 82.2%), and SBP (enzymatic assisted extraction, 48 h, 97.5%; and nitric acid-assisted extraction, 4 h, 83.2%).
26652767	2	12	theme	pectic	487:492	arg1	polysaccharides					494:508	acidic pectic polysaccharides	480:508	acidic pectic polysaccharides	480:508	For acidic pectic polysaccharides, the extraction efficiency was obtained as BP (nitric acid-assisted extraction, 2 h, 62.9%), PP (enzymatic-assisted extraction, 12 h, 75.0%), SBP (enzymatic-assisted extraction, 48 h, 89.8%; and nitric acid-assisted extraction, 4 h, 76.5%), and OH (sodium hexametaphosphate-assisted extraction, 0.5 h, 100%; and ammonium oxalate-assisted extraction, 0.5 h, 100%).
26652767	1	13	theme	onion	409:413	arg1	OH					422:423	OH	422:423	OH	422:423	The influence of different extraction methodologies was assessed on the composition of both neutral (arabinose, rhamnose, galactose) and acidic (galacturonic acid) pectic polysaccharides obtained from four agro-industrial residues, namely, berry pomace (BP), onion hulls (OH), pressed pumpkin (PP), and sugar beet pulp (SBP).
26652767	1	13	theme	onion	409:413	arg1	residues					372:379	four agro-industrial residues	351:379	four agro-industrial residues	351:379	The influence of different extraction methodologies was assessed on the composition of both neutral (arabinose, rhamnose, galactose) and acidic (galacturonic acid) pectic polysaccharides obtained from four agro-industrial residues, namely, berry pomace (BP), onion hulls (OH), pressed pumpkin (PP), and sugar beet pulp (SBP).
26652767	1	13	theme	onion	409:413	arg1	hulls					415:419	onion hulls	409:419	onion hulls (OH)	409:424	The influence of different extraction methodologies was assessed on the composition of both neutral (arabinose, rhamnose, galactose) and acidic (galacturonic acid) pectic polysaccharides obtained from four agro-industrial residues, namely, berry pomace (BP), onion hulls (OH), pressed pumpkin (PP), and sugar beet pulp (SBP).
26652767	1	14	theme	beet	459:462	arg1	residues					372:379	four agro-industrial residues	351:379	four agro-industrial residues	351:379	The influence of different extraction methodologies was assessed on the composition of both neutral (arabinose, rhamnose, galactose) and acidic (galacturonic acid) pectic polysaccharides obtained from four agro-industrial residues, namely, berry pomace (BP), onion hulls (OH), pressed pumpkin (PP), and sugar beet pulp (SBP).
26652767	1	14	theme	beet	459:462	arg1	pulp					464:467	sugar beet pulp	453:467	sugar beet pulp	453:467	The influence of different extraction methodologies was assessed on the composition of both neutral (arabinose, rhamnose, galactose) and acidic (galacturonic acid) pectic polysaccharides obtained from four agro-industrial residues, namely, berry pomace (BP), onion hulls (OH), pressed pumpkin (PP), and sugar beet pulp (SBP).
26652767	2	15	dep	PP	603:604	arg1	extraction					626:635	enzymatic-assisted extraction	607:635	enzymatic-assisted extraction	607:635	For acidic pectic polysaccharides, the extraction efficiency was obtained as BP (nitric acid-assisted extraction, 2 h, 62.9%), PP (enzymatic-assisted extraction, 12 h, 75.0%), SBP (enzymatic-assisted extraction, 48 h, 89.8%; and nitric acid-assisted extraction, 4 h, 76.5%), and OH (sodium hexametaphosphate-assisted extraction, 0.5 h, 100%; and ammonium oxalate-assisted extraction, 0.5 h, 100%).
26652767	2	15	dep	PP	603:604	arg1	h					641:641	12 h	638:641	12 h	638:641	For acidic pectic polysaccharides, the extraction efficiency was obtained as BP (nitric acid-assisted extraction, 2 h, 62.9%), PP (enzymatic-assisted extraction, 12 h, 75.0%), SBP (enzymatic-assisted extraction, 48 h, 89.8%; and nitric acid-assisted extraction, 4 h, 76.5%), and OH (sodium hexametaphosphate-assisted extraction, 0.5 h, 100%; and ammonium oxalate-assisted extraction, 0.5 h, 100%).
26652767	2	15	dep	PP	603:604	arg1	%					648:648	75.0%	644:648	75.0%	644:648	For acidic pectic polysaccharides, the extraction efficiency was obtained as BP (nitric acid-assisted extraction, 2 h, 62.9%), PP (enzymatic-assisted extraction, 12 h, 75.0%), SBP (enzymatic-assisted extraction, 48 h, 89.8%; and nitric acid-assisted extraction, 4 h, 76.5%), and OH (sodium hexametaphosphate-assisted extraction, 0.5 h, 100%; and ammonium oxalate-assisted extraction, 0.5 h, 100%).
26652767	0	16	theme	Crude	90:94	arg1	Extract					103:109	the Crude Pectic Extract	86:109	the Crude Pectic Extract from Various Agro-Industrial Residues	86:147	Effect of Extraction Conditions on the Saccharide (Neutral and Acidic) Composition of the Crude Pectic Extract from Various Agro-Industrial Residues.
26652767	4	17	theme	sugars	1193:1198	arg1	recovery					1174:1181	the high recovery	1165:1181	the high recovery of pectic sugars	1165:1198	On the basis of the high recovery of pectic sugars, SBP and OH are interesting candidates for the further purification of pectin and production of pectin-derived products.
26652767	3	18	dep	BP	947:948	arg1	extraction					970:979	enzymatic-assisted extraction	951:979	enzymatic-assisted extraction	951:979	For neutral pectic polysaccharides, the following results were achieved: BP (enzymatic-assisted extraction, 24 h, 85.9%), PP (nitric acid-assisted extraction, 6 h, 82.2%), and SBP (enzymatic assisted extraction, 48 h, 97.5%; and nitric acid-assisted extraction, 4 h, 83.2%).
26652767	3	18	dep	BP	947:948	arg1	h					985:985	24 h	982:985	24 h	982:985	For neutral pectic polysaccharides, the following results were achieved: BP (enzymatic-assisted extraction, 24 h, 85.9%), PP (nitric acid-assisted extraction, 6 h, 82.2%), and SBP (enzymatic assisted extraction, 48 h, 97.5%; and nitric acid-assisted extraction, 4 h, 83.2%).
26652767	3	18	dep	BP	947:948	arg1	%					992:992	85.9%	988:992	85.9%	988:992	For neutral pectic polysaccharides, the following results were achieved: BP (enzymatic-assisted extraction, 24 h, 85.9%), PP (nitric acid-assisted extraction, 6 h, 82.2%), and SBP (enzymatic assisted extraction, 48 h, 97.5%; and nitric acid-assisted extraction, 4 h, 83.2%).
26652767	0	19	theme	Saccharide	39:48	arg1	Composition					71:81	the Saccharide (Neutral and Acidic) Composition	35:81	the Saccharide (Neutral and Acidic) Composition of the Crude Pectic Extract from Various Agro-Industrial Residues	35:147	Effect of Extraction Conditions on the Saccharide (Neutral and Acidic) Composition of the Crude Pectic Extract from Various Agro-Industrial Residues.
26652767	2	20	theme	hexametaphosphate-assisted	766:791	arg1	extraction					793:802	sodium hexametaphosphate-assisted extraction	759:802	sodium hexametaphosphate-assisted extraction	759:802	For acidic pectic polysaccharides, the extraction efficiency was obtained as BP (nitric acid-assisted extraction, 2 h, 62.9%), PP (enzymatic-assisted extraction, 12 h, 75.0%), SBP (enzymatic-assisted extraction, 48 h, 89.8%; and nitric acid-assisted extraction, 4 h, 76.5%), and OH (sodium hexametaphosphate-assisted extraction, 0.5 h, 100%; and ammonium oxalate-assisted extraction, 0.5 h, 100%).
26652767	2	21	theme	acid-assisted	712:724	arg1	extraction					726:735	nitric acid-assisted extraction	705:735	nitric acid-assisted extraction	705:735	For acidic pectic polysaccharides, the extraction efficiency was obtained as BP (nitric acid-assisted extraction, 2 h, 62.9%), PP (enzymatic-assisted extraction, 12 h, 75.0%), SBP (enzymatic-assisted extraction, 48 h, 89.8%; and nitric acid-assisted extraction, 4 h, 76.5%), and OH (sodium hexametaphosphate-assisted extraction, 0.5 h, 100%; and ammonium oxalate-assisted extraction, 0.5 h, 100%).
26652767	3	22	theme	enzymatic	1055:1063	arg1	extraction					1074:1083	enzymatic assisted extraction	1055:1083	enzymatic assisted extraction	1055:1083	For neutral pectic polysaccharides, the following results were achieved: BP (enzymatic-assisted extraction, 24 h, 85.9%), PP (nitric acid-assisted extraction, 6 h, 82.2%), and SBP (enzymatic assisted extraction, 48 h, 97.5%; and nitric acid-assisted extraction, 4 h, 83.2%).
26652767	3	23	theme	nitric	1000:1005	arg1	extraction					1021:1030	nitric acid-assisted extraction	1000:1030	nitric acid-assisted extraction	1000:1030	For neutral pectic polysaccharides, the following results were achieved: BP (enzymatic-assisted extraction, 24 h, 85.9%), PP (nitric acid-assisted extraction, 6 h, 82.2%), and SBP (enzymatic assisted extraction, 48 h, 97.5%; and nitric acid-assisted extraction, 4 h, 83.2%).
26652767	3	23	theme	nitric	1000:1005	arg1	h					1035:1035	6 h	1033:1035	6 h	1033:1035	For neutral pectic polysaccharides, the following results were achieved: BP (enzymatic-assisted extraction, 24 h, 85.9%), PP (nitric acid-assisted extraction, 6 h, 82.2%), and SBP (enzymatic assisted extraction, 48 h, 97.5%; and nitric acid-assisted extraction, 4 h, 83.2%).
26652767	3	23	theme	nitric	1000:1005	arg1	%					1042:1042	82.2%	1038:1042	82.2%	1038:1042	For neutral pectic polysaccharides, the following results were achieved: BP (enzymatic-assisted extraction, 24 h, 85.9%), PP (nitric acid-assisted extraction, 6 h, 82.2%), and SBP (enzymatic assisted extraction, 48 h, 97.5%; and nitric acid-assisted extraction, 4 h, 83.2%).
26652767	1	24	theme	agro-industrial	356:370	arg1	pomace					396:401	berry pomace	390:401	berry pomace (BP)	390:406	The influence of different extraction methodologies was assessed on the composition of both neutral (arabinose, rhamnose, galactose) and acidic (galacturonic acid) pectic polysaccharides obtained from four agro-industrial residues, namely, berry pomace (BP), onion hulls (OH), pressed pumpkin (PP), and sugar beet pulp (SBP).
26652767	1	24	theme	agro-industrial	356:370	arg1	residues					372:379	four agro-industrial residues	351:379	four agro-industrial residues	351:379	The influence of different extraction methodologies was assessed on the composition of both neutral (arabinose, rhamnose, galactose) and acidic (galacturonic acid) pectic polysaccharides obtained from four agro-industrial residues, namely, berry pomace (BP), onion hulls (OH), pressed pumpkin (PP), and sugar beet pulp (SBP).
26652767	1	24	theme	agro-industrial	356:370	arg1	hulls					415:419	onion hulls	409:419	onion hulls (OH)	409:424	The influence of different extraction methodologies was assessed on the composition of both neutral (arabinose, rhamnose, galactose) and acidic (galacturonic acid) pectic polysaccharides obtained from four agro-industrial residues, namely, berry pomace (BP), onion hulls (OH), pressed pumpkin (PP), and sugar beet pulp (SBP).
26652767	1	24	theme	agro-industrial	356:370	arg1	pumpkin					435:441	pressed pumpkin	427:441	pressed pumpkin (PP)	427:446	The influence of different extraction methodologies was assessed on the composition of both neutral (arabinose, rhamnose, galactose) and acidic (galacturonic acid) pectic polysaccharides obtained from four agro-industrial residues, namely, berry pomace (BP), onion hulls (OH), pressed pumpkin (PP), and sugar beet pulp (SBP).
26652767	1	24	theme	agro-industrial	356:370	arg1	pulp					464:467	sugar beet pulp	453:467	sugar beet pulp	453:467	The influence of different extraction methodologies was assessed on the composition of both neutral (arabinose, rhamnose, galactose) and acidic (galacturonic acid) pectic polysaccharides obtained from four agro-industrial residues, namely, berry pomace (BP), onion hulls (OH), pressed pumpkin (PP), and sugar beet pulp (SBP).
26652767	4	25	theme	products	1311:1318	arg1	pectin					1271:1276	pectin	1271:1276	pectin	1271:1276	On the basis of the high recovery of pectic sugars, SBP and OH are interesting candidates for the further purification of pectin and production of pectin-derived products.
26652767	4	25	theme	products	1311:1318	arg1	production					1282:1291	production	1282:1291	production	1282:1291	On the basis of the high recovery of pectic sugars, SBP and OH are interesting candidates for the further purification of pectin and production of pectin-derived products.
26652767	2	26	theme	enzymatic-assisted	607:624	arg1	extraction					626:635	enzymatic-assisted extraction	607:635	enzymatic-assisted extraction	607:635	For acidic pectic polysaccharides, the extraction efficiency was obtained as BP (nitric acid-assisted extraction, 2 h, 62.9%), PP (enzymatic-assisted extraction, 12 h, 75.0%), SBP (enzymatic-assisted extraction, 48 h, 89.8%; and nitric acid-assisted extraction, 4 h, 76.5%), and OH (sodium hexametaphosphate-assisted extraction, 0.5 h, 100%; and ammonium oxalate-assisted extraction, 0.5 h, 100%).
26652767	2	26	theme	enzymatic-assisted	607:624	arg1	h					641:641	12 h	638:641	12 h	638:641	For acidic pectic polysaccharides, the extraction efficiency was obtained as BP (nitric acid-assisted extraction, 2 h, 62.9%), PP (enzymatic-assisted extraction, 12 h, 75.0%), SBP (enzymatic-assisted extraction, 48 h, 89.8%; and nitric acid-assisted extraction, 4 h, 76.5%), and OH (sodium hexametaphosphate-assisted extraction, 0.5 h, 100%; and ammonium oxalate-assisted extraction, 0.5 h, 100%).
26652767	2	26	theme	enzymatic-assisted	607:624	arg1	%					648:648	75.0%	644:648	75.0%	644:648	For acidic pectic polysaccharides, the extraction efficiency was obtained as BP (nitric acid-assisted extraction, 2 h, 62.9%), PP (enzymatic-assisted extraction, 12 h, 75.0%), SBP (enzymatic-assisted extraction, 48 h, 89.8%; and nitric acid-assisted extraction, 4 h, 76.5%), and OH (sodium hexametaphosphate-assisted extraction, 0.5 h, 100%; and ammonium oxalate-assisted extraction, 0.5 h, 100%).
26652767	1	27	theme	different	167:175	arg1	methodologies					188:200	different extraction methodologies	167:200	different extraction methodologies	167:200	The influence of different extraction methodologies was assessed on the composition of both neutral (arabinose, rhamnose, galactose) and acidic (galacturonic acid) pectic polysaccharides obtained from four agro-industrial residues, namely, berry pomace (BP), onion hulls (OH), pressed pumpkin (PP), and sugar beet pulp (SBP).
26652767	2	28	theme	extraction	515:524	arg1	SBP					652:654	SBP	652:654	SBP (enzymatic-assisted extraction, 48 h, 89.8%; and nitric acid-assisted extraction, 4 h, 76.5%)	652:748	For acidic pectic polysaccharides, the extraction efficiency was obtained as BP (nitric acid-assisted extraction, 2 h, 62.9%), PP (enzymatic-assisted extraction, 12 h, 75.0%), SBP (enzymatic-assisted extraction, 48 h, 89.8%; and nitric acid-assisted extraction, 4 h, 76.5%), and OH (sodium hexametaphosphate-assisted extraction, 0.5 h, 100%; and ammonium oxalate-assisted extraction, 0.5 h, 100%).
26652767	2	28	theme	extraction	515:524	arg1	efficiency					526:535	the extraction efficiency	511:535	the extraction efficiency	511:535	For acidic pectic polysaccharides, the extraction efficiency was obtained as BP (nitric acid-assisted extraction, 2 h, 62.9%), PP (enzymatic-assisted extraction, 12 h, 75.0%), SBP (enzymatic-assisted extraction, 48 h, 89.8%; and nitric acid-assisted extraction, 4 h, 76.5%), and OH (sodium hexametaphosphate-assisted extraction, 0.5 h, 100%; and ammonium oxalate-assisted extraction, 0.5 h, 100%).
26652767	2	28	theme	extraction	515:524	arg1	PP					603:604	PP	603:604	PP (enzymatic-assisted extraction, 12 h, 75.0%)	603:649	For acidic pectic polysaccharides, the extraction efficiency was obtained as BP (nitric acid-assisted extraction, 2 h, 62.9%), PP (enzymatic-assisted extraction, 12 h, 75.0%), SBP (enzymatic-assisted extraction, 48 h, 89.8%; and nitric acid-assisted extraction, 4 h, 76.5%), and OH (sodium hexametaphosphate-assisted extraction, 0.5 h, 100%; and ammonium oxalate-assisted extraction, 0.5 h, 100%).
26652767	2	28	theme	extraction	515:524	arg1	BP					553:554	BP	553:554	BP (nitric acid-assisted extraction, 2 h, 62.9%)	553:600	For acidic pectic polysaccharides, the extraction efficiency was obtained as BP (nitric acid-assisted extraction, 2 h, 62.9%), PP (enzymatic-assisted extraction, 12 h, 75.0%), SBP (enzymatic-assisted extraction, 48 h, 89.8%; and nitric acid-assisted extraction, 4 h, 76.5%), and OH (sodium hexametaphosphate-assisted extraction, 0.5 h, 100%; and ammonium oxalate-assisted extraction, 0.5 h, 100%).
26652767	2	28	theme	extraction	515:524	arg1	OH					755:756	OH	755:756	OH (sodium hexametaphosphate-assisted extraction, 0.5 h, 100%; and ammonium oxalate-assisted extraction, 0.5 h, 100%)	755:871	For acidic pectic polysaccharides, the extraction efficiency was obtained as BP (nitric acid-assisted extraction, 2 h, 62.9%), PP (enzymatic-assisted extraction, 12 h, 75.0%), SBP (enzymatic-assisted extraction, 48 h, 89.8%; and nitric acid-assisted extraction, 4 h, 76.5%), and OH (sodium hexametaphosphate-assisted extraction, 0.5 h, 100%; and ammonium oxalate-assisted extraction, 0.5 h, 100%).
26652767	2	29	theme	ammonium	822:829	arg1	extraction					848:857	ammonium oxalate-assisted extraction	822:857	ammonium oxalate-assisted extraction	822:857	For acidic pectic polysaccharides, the extraction efficiency was obtained as BP (nitric acid-assisted extraction, 2 h, 62.9%), PP (enzymatic-assisted extraction, 12 h, 75.0%), SBP (enzymatic-assisted extraction, 48 h, 89.8%; and nitric acid-assisted extraction, 4 h, 76.5%), and OH (sodium hexametaphosphate-assisted extraction, 0.5 h, 100%; and ammonium oxalate-assisted extraction, 0.5 h, 100%).
26652767	1	30	theme	extraction	177:186	arg1	methodologies					188:200	different extraction methodologies	167:200	different extraction methodologies	167:200	The influence of different extraction methodologies was assessed on the composition of both neutral (arabinose, rhamnose, galactose) and acidic (galacturonic acid) pectic polysaccharides obtained from four agro-industrial residues, namely, berry pomace (BP), onion hulls (OH), pressed pumpkin (PP), and sugar beet pulp (SBP).
26652767	0	31	theme	Conditions	21:30	arg1	Effect					0:5	Effect	0:5	Effect of Extraction Conditions on the Saccharide (Neutral and Acidic) Composition of the Crude Pectic Extract from Various Agro-Industrial Residues.	0:148	Effect of Extraction Conditions on the Saccharide (Neutral and Acidic) Composition of the Crude Pectic Extract from Various Agro-Industrial Residues.
26652767	0	32	theme	Extract	103:109	arg1	Composition					71:81	the Saccharide (Neutral and Acidic) Composition	35:81	the Saccharide (Neutral and Acidic) Composition of the Crude Pectic Extract from Various Agro-Industrial Residues	35:147	Effect of Extraction Conditions on the Saccharide (Neutral and Acidic) Composition of the Crude Pectic Extract from Various Agro-Industrial Residues.
26652767	2	33	theme	acid-assisted	564:576	arg1	extraction					578:587	nitric acid-assisted extraction	557:587	nitric acid-assisted extraction	557:587	For acidic pectic polysaccharides, the extraction efficiency was obtained as BP (nitric acid-assisted extraction, 2 h, 62.9%), PP (enzymatic-assisted extraction, 12 h, 75.0%), SBP (enzymatic-assisted extraction, 48 h, 89.8%; and nitric acid-assisted extraction, 4 h, 76.5%), and OH (sodium hexametaphosphate-assisted extraction, 0.5 h, 100%; and ammonium oxalate-assisted extraction, 0.5 h, 100%).
26652767	2	33	theme	acid-assisted	564:576	arg1	h					592:592	2 h	590:592	2 h	590:592	For acidic pectic polysaccharides, the extraction efficiency was obtained as BP (nitric acid-assisted extraction, 2 h, 62.9%), PP (enzymatic-assisted extraction, 12 h, 75.0%), SBP (enzymatic-assisted extraction, 48 h, 89.8%; and nitric acid-assisted extraction, 4 h, 76.5%), and OH (sodium hexametaphosphate-assisted extraction, 0.5 h, 100%; and ammonium oxalate-assisted extraction, 0.5 h, 100%).
26652767	2	33	theme	acid-assisted	564:576	arg1	%					599:599	62.9%	595:599	62.9%	595:599	For acidic pectic polysaccharides, the extraction efficiency was obtained as BP (nitric acid-assisted extraction, 2 h, 62.9%), PP (enzymatic-assisted extraction, 12 h, 75.0%), SBP (enzymatic-assisted extraction, 48 h, 89.8%; and nitric acid-assisted extraction, 4 h, 76.5%), and OH (sodium hexametaphosphate-assisted extraction, 0.5 h, 100%; and ammonium oxalate-assisted extraction, 0.5 h, 100%).
26652767	4	34	dep	recovery	1174:1181	arg1	the					1152:1154	the	1152:1154	the	1152:1154	On the basis of the high recovery of pectic sugars, SBP and OH are interesting candidates for the further purification of pectin and production of pectin-derived products.
26652767	4	34	dep	recovery	1174:1181	arg1	basis					1156:1160	basis	1156:1160	basis	1156:1160	On the basis of the high recovery of pectic sugars, SBP and OH are interesting candidates for the further purification of pectin and production of pectin-derived products.
26652767	1	35	theme	methodologies	188:200	arg1	influence					154:162	The influence	150:162	The influence of different extraction methodologies	150:200	The influence of different extraction methodologies was assessed on the composition of both neutral (arabinose, rhamnose, galactose) and acidic (galacturonic acid) pectic polysaccharides obtained from four agro-industrial residues, namely, berry pomace (BP), onion hulls (OH), pressed pumpkin (PP), and sugar beet pulp (SBP).
26652767	0	36	theme	Extraction	10:19	arg1	Conditions					21:30	Extraction Conditions	10:30	Extraction Conditions	10:30	Effect of Extraction Conditions on the Saccharide (Neutral and Acidic) Composition of the Crude Pectic Extract from Various Agro-Industrial Residues.
26652767	1	37	theme	galactose	272:280	arg1	composition					222:232	the composition	218:232	the composition of both neutral (arabinose, rhamnose, galactose) and acidic (galacturonic acid) pectic polysaccharides obtained from four agro-industrial residues, namely, berry pomace (BP), onion hulls (OH), pressed pumpkin (PP), and sugar beet pulp (SBP)	218:473	The influence of different extraction methodologies was assessed on the composition of both neutral (arabinose, rhamnose, galactose) and acidic (galacturonic acid) pectic polysaccharides obtained from four agro-industrial residues, namely, berry pomace (BP), onion hulls (OH), pressed pumpkin (PP), and sugar beet pulp (SBP).
26652767	0	38	theme	Pectic	96:101	arg1	Extract					103:109	the Crude Pectic Extract	86:109	the Crude Pectic Extract from Various Agro-Industrial Residues	86:147	Effect of Extraction Conditions on the Saccharide (Neutral and Acidic) Composition of the Crude Pectic Extract from Various Agro-Industrial Residues.
26652767	3	39	dep	SBP	1050:1052	arg1	h					1138:1138	4 h	1136:1138	4 h	1136:1138	For neutral pectic polysaccharides, the following results were achieved: BP (enzymatic-assisted extraction, 24 h, 85.9%), PP (nitric acid-assisted extraction, 6 h, 82.2%), and SBP (enzymatic assisted extraction, 48 h, 97.5%; and nitric acid-assisted extraction, 4 h, 83.2%).
26652767	3	39	dep	SBP	1050:1052	arg1	h					1089:1089	48 h	1086:1089	48 h	1086:1089	For neutral pectic polysaccharides, the following results were achieved: BP (enzymatic-assisted extraction, 24 h, 85.9%), PP (nitric acid-assisted extraction, 6 h, 82.2%), and SBP (enzymatic assisted extraction, 48 h, 97.5%; and nitric acid-assisted extraction, 4 h, 83.2%).
26652767	3	39	dep	SBP	1050:1052	arg1	extraction					1124:1133	nitric acid-assisted extraction	1103:1133	nitric acid-assisted extraction	1103:1133	For neutral pectic polysaccharides, the following results were achieved: BP (enzymatic-assisted extraction, 24 h, 85.9%), PP (nitric acid-assisted extraction, 6 h, 82.2%), and SBP (enzymatic assisted extraction, 48 h, 97.5%; and nitric acid-assisted extraction, 4 h, 83.2%).
26652767	3	39	dep	SBP	1050:1052	arg1	%					1145:1145	83.2%	1141:1145	83.2%	1141:1145	For neutral pectic polysaccharides, the following results were achieved: BP (enzymatic-assisted extraction, 24 h, 85.9%), PP (nitric acid-assisted extraction, 6 h, 82.2%), and SBP (enzymatic assisted extraction, 48 h, 97.5%; and nitric acid-assisted extraction, 4 h, 83.2%).
26652767	3	39	dep	SBP	1050:1052	arg1	extraction					1074:1083	enzymatic assisted extraction	1055:1083	enzymatic assisted extraction	1055:1083	For neutral pectic polysaccharides, the following results were achieved: BP (enzymatic-assisted extraction, 24 h, 85.9%), PP (nitric acid-assisted extraction, 6 h, 82.2%), and SBP (enzymatic assisted extraction, 48 h, 97.5%; and nitric acid-assisted extraction, 4 h, 83.2%).
26652767	3	39	dep	SBP	1050:1052	arg1	%					1096:1096	97.5%	1092:1096	97.5%	1092:1096	For neutral pectic polysaccharides, the following results were achieved: BP (enzymatic-assisted extraction, 24 h, 85.9%), PP (nitric acid-assisted extraction, 6 h, 82.2%), and SBP (enzymatic assisted extraction, 48 h, 97.5%; and nitric acid-assisted extraction, 4 h, 83.2%).
26652767	2	40	dep	extraction	793:802	arg1	h					864:864	0.5 h	860:864	0.5 h	860:864	For acidic pectic polysaccharides, the extraction efficiency was obtained as BP (nitric acid-assisted extraction, 2 h, 62.9%), PP (enzymatic-assisted extraction, 12 h, 75.0%), SBP (enzymatic-assisted extraction, 48 h, 89.8%; and nitric acid-assisted extraction, 4 h, 76.5%), and OH (sodium hexametaphosphate-assisted extraction, 0.5 h, 100%; and ammonium oxalate-assisted extraction, 0.5 h, 100%).
26652767	2	40	dep	extraction	793:802	arg1	extraction					848:857	ammonium oxalate-assisted extraction	822:857	ammonium oxalate-assisted extraction	822:857	For acidic pectic polysaccharides, the extraction efficiency was obtained as BP (nitric acid-assisted extraction, 2 h, 62.9%), PP (enzymatic-assisted extraction, 12 h, 75.0%), SBP (enzymatic-assisted extraction, 48 h, 89.8%; and nitric acid-assisted extraction, 4 h, 76.5%), and OH (sodium hexametaphosphate-assisted extraction, 0.5 h, 100%; and ammonium oxalate-assisted extraction, 0.5 h, 100%).
26652767	2	40	dep	extraction	793:802	arg1	h					809:809	0.5 h	805:809	0.5 h	805:809	For acidic pectic polysaccharides, the extraction efficiency was obtained as BP (nitric acid-assisted extraction, 2 h, 62.9%), PP (enzymatic-assisted extraction, 12 h, 75.0%), SBP (enzymatic-assisted extraction, 48 h, 89.8%; and nitric acid-assisted extraction, 4 h, 76.5%), and OH (sodium hexametaphosphate-assisted extraction, 0.5 h, 100%; and ammonium oxalate-assisted extraction, 0.5 h, 100%).
26652767	2	40	dep	extraction	793:802	arg1	%					815:815	100%	812:815	100%	812:815	For acidic pectic polysaccharides, the extraction efficiency was obtained as BP (nitric acid-assisted extraction, 2 h, 62.9%), PP (enzymatic-assisted extraction, 12 h, 75.0%), SBP (enzymatic-assisted extraction, 48 h, 89.8%; and nitric acid-assisted extraction, 4 h, 76.5%), and OH (sodium hexametaphosphate-assisted extraction, 0.5 h, 100%; and ammonium oxalate-assisted extraction, 0.5 h, 100%).
26652767	2	40	dep	extraction	793:802	arg1	%					870:870	100%	867:870	100%	867:870	For acidic pectic polysaccharides, the extraction efficiency was obtained as BP (nitric acid-assisted extraction, 2 h, 62.9%), PP (enzymatic-assisted extraction, 12 h, 75.0%), SBP (enzymatic-assisted extraction, 48 h, 89.8%; and nitric acid-assisted extraction, 4 h, 76.5%), and OH (sodium hexametaphosphate-assisted extraction, 0.5 h, 100%; and ammonium oxalate-assisted extraction, 0.5 h, 100%).
26652767	0	41	theme	Various	116:122	arg1	Residues					140:147	Various Agro-Industrial Residues	116:147	Various Agro-Industrial Residues	116:147	Effect of Extraction Conditions on the Saccharide (Neutral and Acidic) Composition of the Crude Pectic Extract from Various Agro-Industrial Residues.
26652767	4	42	theme	pectin	1271:1276	arg1	purification					1255:1266	the further purification	1243:1266	the further purification of pectin and production of pectin-derived products	1243:1318	On the basis of the high recovery of pectic sugars, SBP and OH are interesting candidates for the further purification of pectin and production of pectin-derived products.
26652767	3	43	theme	acid-assisted	1007:1019	arg1	extraction					1021:1030	nitric acid-assisted extraction	1000:1030	nitric acid-assisted extraction	1000:1030	For neutral pectic polysaccharides, the following results were achieved: BP (enzymatic-assisted extraction, 24 h, 85.9%), PP (nitric acid-assisted extraction, 6 h, 82.2%), and SBP (enzymatic assisted extraction, 48 h, 97.5%; and nitric acid-assisted extraction, 4 h, 83.2%).
26652767	3	43	theme	acid-assisted	1007:1019	arg1	h					1035:1035	6 h	1033:1035	6 h	1033:1035	For neutral pectic polysaccharides, the following results were achieved: BP (enzymatic-assisted extraction, 24 h, 85.9%), PP (nitric acid-assisted extraction, 6 h, 82.2%), and SBP (enzymatic assisted extraction, 48 h, 97.5%; and nitric acid-assisted extraction, 4 h, 83.2%).
26652767	3	43	theme	acid-assisted	1007:1019	arg1	%					1042:1042	82.2%	1038:1042	82.2%	1038:1042	For neutral pectic polysaccharides, the following results were achieved: BP (enzymatic-assisted extraction, 24 h, 85.9%), PP (nitric acid-assisted extraction, 6 h, 82.2%), and SBP (enzymatic assisted extraction, 48 h, 97.5%; and nitric acid-assisted extraction, 4 h, 83.2%).
26652767	4	44	theme	pectin-derived	1296:1309	arg1	products					1311:1318	pectin-derived products	1296:1318	pectin-derived products	1296:1318	On the basis of the high recovery of pectic sugars, SBP and OH are interesting candidates for the further purification of pectin and production of pectin-derived products.
26652767	2	45	dep	SBP	652:654	arg1	h					691:691	48 h	688:691	48 h	688:691	For acidic pectic polysaccharides, the extraction efficiency was obtained as BP (nitric acid-assisted extraction, 2 h, 62.9%), PP (enzymatic-assisted extraction, 12 h, 75.0%), SBP (enzymatic-assisted extraction, 48 h, 89.8%; and nitric acid-assisted extraction, 4 h, 76.5%), and OH (sodium hexametaphosphate-assisted extraction, 0.5 h, 100%; and ammonium oxalate-assisted extraction, 0.5 h, 100%).
26652767	2	45	dep	SBP	652:654	arg1	%					747:747	76.5%	743:747	76.5%	743:747	For acidic pectic polysaccharides, the extraction efficiency was obtained as BP (nitric acid-assisted extraction, 2 h, 62.9%), PP (enzymatic-assisted extraction, 12 h, 75.0%), SBP (enzymatic-assisted extraction, 48 h, 89.8%; and nitric acid-assisted extraction, 4 h, 76.5%), and OH (sodium hexametaphosphate-assisted extraction, 0.5 h, 100%; and ammonium oxalate-assisted extraction, 0.5 h, 100%).
26652767	2	45	dep	SBP	652:654	arg1	extraction					726:735	nitric acid-assisted extraction	705:735	nitric acid-assisted extraction	705:735	For acidic pectic polysaccharides, the extraction efficiency was obtained as BP (nitric acid-assisted extraction, 2 h, 62.9%), PP (enzymatic-assisted extraction, 12 h, 75.0%), SBP (enzymatic-assisted extraction, 48 h, 89.8%; and nitric acid-assisted extraction, 4 h, 76.5%), and OH (sodium hexametaphosphate-assisted extraction, 0.5 h, 100%; and ammonium oxalate-assisted extraction, 0.5 h, 100%).
26652767	2	45	dep	SBP	652:654	arg1	%					698:698	89.8%	694:698	89.8%	694:698	For acidic pectic polysaccharides, the extraction efficiency was obtained as BP (nitric acid-assisted extraction, 2 h, 62.9%), PP (enzymatic-assisted extraction, 12 h, 75.0%), SBP (enzymatic-assisted extraction, 48 h, 89.8%; and nitric acid-assisted extraction, 4 h, 76.5%), and OH (sodium hexametaphosphate-assisted extraction, 0.5 h, 100%; and ammonium oxalate-assisted extraction, 0.5 h, 100%).
26652767	2	45	dep	SBP	652:654	arg1	extraction					676:685	enzymatic-assisted extraction	657:685	enzymatic-assisted extraction	657:685	For acidic pectic polysaccharides, the extraction efficiency was obtained as BP (nitric acid-assisted extraction, 2 h, 62.9%), PP (enzymatic-assisted extraction, 12 h, 75.0%), SBP (enzymatic-assisted extraction, 48 h, 89.8%; and nitric acid-assisted extraction, 4 h, 76.5%), and OH (sodium hexametaphosphate-assisted extraction, 0.5 h, 100%; and ammonium oxalate-assisted extraction, 0.5 h, 100%).
26652767	2	45	dep	SBP	652:654	arg1	h					740:740	4 h	738:740	4 h	738:740	For acidic pectic polysaccharides, the extraction efficiency was obtained as BP (nitric acid-assisted extraction, 2 h, 62.9%), PP (enzymatic-assisted extraction, 12 h, 75.0%), SBP (enzymatic-assisted extraction, 48 h, 89.8%; and nitric acid-assisted extraction, 4 h, 76.5%), and OH (sodium hexametaphosphate-assisted extraction, 0.5 h, 100%; and ammonium oxalate-assisted extraction, 0.5 h, 100%).
26652767	0	46	dep	Saccharide	39:48	arg1	Acidic					63:68	Acidic	63:68	Acidic	63:68	Effect of Extraction Conditions on the Saccharide (Neutral and Acidic) Composition of the Crude Pectic Extract from Various Agro-Industrial Residues.
26652767	0	46	dep	Saccharide	39:48	arg1	Neutral					51:57	Neutral	51:57	Neutral	51:57	Effect of Extraction Conditions on the Saccharide (Neutral and Acidic) Composition of the Crude Pectic Extract from Various Agro-Industrial Residues.
26652767	1	47	theme	pressed	427:433	arg1	residues					372:379	four agro-industrial residues	351:379	four agro-industrial residues	351:379	The influence of different extraction methodologies was assessed on the composition of both neutral (arabinose, rhamnose, galactose) and acidic (galacturonic acid) pectic polysaccharides obtained from four agro-industrial residues, namely, berry pomace (BP), onion hulls (OH), pressed pumpkin (PP), and sugar beet pulp (SBP).
26652767	1	47	theme	pressed	427:433	arg1	PP					444:445	PP	444:445	PP	444:445	The influence of different extraction methodologies was assessed on the composition of both neutral (arabinose, rhamnose, galactose) and acidic (galacturonic acid) pectic polysaccharides obtained from four agro-industrial residues, namely, berry pomace (BP), onion hulls (OH), pressed pumpkin (PP), and sugar beet pulp (SBP).
26652767	1	47	theme	pressed	427:433	arg1	pumpkin					435:441	pressed pumpkin	427:441	pressed pumpkin (PP)	427:446	The influence of different extraction methodologies was assessed on the composition of both neutral (arabinose, rhamnose, galactose) and acidic (galacturonic acid) pectic polysaccharides obtained from four agro-industrial residues, namely, berry pomace (BP), onion hulls (OH), pressed pumpkin (PP), and sugar beet pulp (SBP).
26652767	2	48	dep	OH	755:756	arg1	extraction					793:802	sodium hexametaphosphate-assisted extraction	759:802	sodium hexametaphosphate-assisted extraction	759:802	For acidic pectic polysaccharides, the extraction efficiency was obtained as BP (nitric acid-assisted extraction, 2 h, 62.9%), PP (enzymatic-assisted extraction, 12 h, 75.0%), SBP (enzymatic-assisted extraction, 48 h, 89.8%; and nitric acid-assisted extraction, 4 h, 76.5%), and OH (sodium hexametaphosphate-assisted extraction, 0.5 h, 100%; and ammonium oxalate-assisted extraction, 0.5 h, 100%).
26652767	4	49	link	pectin-derived	1296:1309	arg1	products					1311:1318	pectin-derived products	1296:1318	pectin-derived products	1296:1318	On the basis of the high recovery of pectic sugars, SBP and OH are interesting candidates for the further purification of pectin and production of pectin-derived products.
26652767	1	50	theme	acidic	287:292	arg1	acid					308:311	galacturonic acid	295:311	galacturonic acid	295:311	The influence of different extraction methodologies was assessed on the composition of both neutral (arabinose, rhamnose, galactose) and acidic (galacturonic acid) pectic polysaccharides obtained from four agro-industrial residues, namely, berry pomace (BP), onion hulls (OH), pressed pumpkin (PP), and sugar beet pulp (SBP).
26652767	1	50	theme	acidic	287:292	arg1	polysaccharides					321:335	both neutral (arabinose, rhamnose, galactose) and acidic (galacturonic acid) pectic polysaccharides	237:335	polysaccharides	321:335	The influence of different extraction methodologies was assessed on the composition of both neutral (arabinose, rhamnose, galactose) and acidic (galacturonic acid) pectic polysaccharides obtained from four agro-industrial residues, namely, berry pomace (BP), onion hulls (OH), pressed pumpkin (PP), and sugar beet pulp (SBP).
26652767	2	51	theme	acidic	480:485	arg1	polysaccharides					494:508	acidic pectic polysaccharides	480:508	acidic pectic polysaccharides	480:508	For acidic pectic polysaccharides, the extraction efficiency was obtained as BP (nitric acid-assisted extraction, 2 h, 62.9%), PP (enzymatic-assisted extraction, 12 h, 75.0%), SBP (enzymatic-assisted extraction, 48 h, 89.8%; and nitric acid-assisted extraction, 4 h, 76.5%), and OH (sodium hexametaphosphate-assisted extraction, 0.5 h, 100%; and ammonium oxalate-assisted extraction, 0.5 h, 100%).
26652767	4	52	theme	production	1282:1291	arg1	purification					1255:1266	the further purification	1243:1266	the further purification of pectin and production of pectin-derived products	1243:1318	On the basis of the high recovery of pectic sugars, SBP and OH are interesting candidates for the further purification of pectin and production of pectin-derived products.
26652767	4	53	theme	recovery	1174:1181	arg1	SBP					1201:1203	SBP	1201:1203	SBP	1201:1203	On the basis of the high recovery of pectic sugars, SBP and OH are interesting candidates for the further purification of pectin and production of pectin-derived products.
26652767	4	53	theme	recovery	1174:1181	arg1	OH					1209:1210	OH	1209:1210	OH	1209:1210	On the basis of the high recovery of pectic sugars, SBP and OH are interesting candidates for the further purification of pectin and production of pectin-derived products.
26652767	4	53	theme	recovery	1174:1181	arg1	candidates					1228:1237	interesting candidates	1216:1237	interesting candidates for the further purification of pectin and production of pectin-derived products	1216:1318	On the basis of the high recovery of pectic sugars, SBP and OH are interesting candidates for the further purification of pectin and production of pectin-derived products.
26652767	0	54	from	Effect	0:5	arg1	Composition					71:81	the Saccharide (Neutral and Acidic) Composition	35:81	the Saccharide (Neutral and Acidic) Composition of the Crude Pectic Extract from Various Agro-Industrial Residues	35:147	Effect of Extraction Conditions on the Saccharide (Neutral and Acidic) Composition of the Crude Pectic Extract from Various Agro-Industrial Residues.
26652767	0	55	theme	Agro-Industrial	124:138	arg1	Residues					140:147	Various Agro-Industrial Residues	116:147	Various Agro-Industrial Residues	116:147	Effect of Extraction Conditions on the Saccharide (Neutral and Acidic) Composition of the Crude Pectic Extract from Various Agro-Industrial Residues.
26652767	1	56	theme	berry	390:394	arg1	pomace					396:401	berry pomace	390:401	berry pomace (BP)	390:406	The influence of different extraction methodologies was assessed on the composition of both neutral (arabinose, rhamnose, galactose) and acidic (galacturonic acid) pectic polysaccharides obtained from four agro-industrial residues, namely, berry pomace (BP), onion hulls (OH), pressed pumpkin (PP), and sugar beet pulp (SBP).
26652767	1	56	theme	berry	390:394	arg1	residues					372:379	four agro-industrial residues	351:379	four agro-industrial residues	351:379	The influence of different extraction methodologies was assessed on the composition of both neutral (arabinose, rhamnose, galactose) and acidic (galacturonic acid) pectic polysaccharides obtained from four agro-industrial residues, namely, berry pomace (BP), onion hulls (OH), pressed pumpkin (PP), and sugar beet pulp (SBP).
26652767	1	56	theme	berry	390:394	arg1	SBP					470:472	SBP	470:472	SBP	470:472	The influence of different extraction methodologies was assessed on the composition of both neutral (arabinose, rhamnose, galactose) and acidic (galacturonic acid) pectic polysaccharides obtained from four agro-industrial residues, namely, berry pomace (BP), onion hulls (OH), pressed pumpkin (PP), and sugar beet pulp (SBP).
26652767	1	56	theme	berry	390:394	arg1	BP					404:405	BP	404:405	BP	404:405	The influence of different extraction methodologies was assessed on the composition of both neutral (arabinose, rhamnose, galactose) and acidic (galacturonic acid) pectic polysaccharides obtained from four agro-industrial residues, namely, berry pomace (BP), onion hulls (OH), pressed pumpkin (PP), and sugar beet pulp (SBP).
26652767	2	57	theme	oxalate-assisted	831:846	arg1	extraction					848:857	ammonium oxalate-assisted extraction	822:857	ammonium oxalate-assisted extraction	822:857	For acidic pectic polysaccharides, the extraction efficiency was obtained as BP (nitric acid-assisted extraction, 2 h, 62.9%), PP (enzymatic-assisted extraction, 12 h, 75.0%), SBP (enzymatic-assisted extraction, 48 h, 89.8%; and nitric acid-assisted extraction, 4 h, 76.5%), and OH (sodium hexametaphosphate-assisted extraction, 0.5 h, 100%; and ammonium oxalate-assisted extraction, 0.5 h, 100%).
26652767	4	58	theme	high	1169:1172	arg1	recovery					1174:1181	the high recovery	1165:1181	the high recovery of pectic sugars	1165:1198	On the basis of the high recovery of pectic sugars, SBP and OH are interesting candidates for the further purification of pectin and production of pectin-derived products.
26652767	1	59	theme	galacturonic	295:306	arg1	acid					308:311	galacturonic acid	295:311	galacturonic acid	295:311	The influence of different extraction methodologies was assessed on the composition of both neutral (arabinose, rhamnose, galactose) and acidic (galacturonic acid) pectic polysaccharides obtained from four agro-industrial residues, namely, berry pomace (BP), onion hulls (OH), pressed pumpkin (PP), and sugar beet pulp (SBP).
26652767	1	59	theme	galacturonic	295:306	arg1	polysaccharides					321:335	both neutral (arabinose, rhamnose, galactose) and acidic (galacturonic acid) pectic polysaccharides	237:335	polysaccharides	321:335	The influence of different extraction methodologies was assessed on the composition of both neutral (arabinose, rhamnose, galactose) and acidic (galacturonic acid) pectic polysaccharides obtained from four agro-industrial residues, namely, berry pomace (BP), onion hulls (OH), pressed pumpkin (PP), and sugar beet pulp (SBP).
26652767	3	60	theme	pectic	886:891	arg1	polysaccharides					893:907	neutral pectic polysaccharides	878:907	neutral pectic polysaccharides	878:907	For neutral pectic polysaccharides, the following results were achieved: BP (enzymatic-assisted extraction, 24 h, 85.9%), PP (nitric acid-assisted extraction, 6 h, 82.2%), and SBP (enzymatic assisted extraction, 48 h, 97.5%; and nitric acid-assisted extraction, 4 h, 83.2%).
26652767	2	61	theme	sodium	759:764	arg1	extraction					793:802	sodium hexametaphosphate-assisted extraction	759:802	sodium hexametaphosphate-assisted extraction	759:802	For acidic pectic polysaccharides, the extraction efficiency was obtained as BP (nitric acid-assisted extraction, 2 h, 62.9%), PP (enzymatic-assisted extraction, 12 h, 75.0%), SBP (enzymatic-assisted extraction, 48 h, 89.8%; and nitric acid-assisted extraction, 4 h, 76.5%), and OH (sodium hexametaphosphate-assisted extraction, 0.5 h, 100%; and ammonium oxalate-assisted extraction, 0.5 h, 100%).
26652767	3	62	dep	PP	996:997	arg1	extraction					1021:1030	nitric acid-assisted extraction	1000:1030	nitric acid-assisted extraction	1000:1030	For neutral pectic polysaccharides, the following results were achieved: BP (enzymatic-assisted extraction, 24 h, 85.9%), PP (nitric acid-assisted extraction, 6 h, 82.2%), and SBP (enzymatic assisted extraction, 48 h, 97.5%; and nitric acid-assisted extraction, 4 h, 83.2%).
26652767	3	62	dep	PP	996:997	arg1	h					1035:1035	6 h	1033:1035	6 h	1033:1035	For neutral pectic polysaccharides, the following results were achieved: BP (enzymatic-assisted extraction, 24 h, 85.9%), PP (nitric acid-assisted extraction, 6 h, 82.2%), and SBP (enzymatic assisted extraction, 48 h, 97.5%; and nitric acid-assisted extraction, 4 h, 83.2%).
26652767	3	62	dep	PP	996:997	arg1	%					1042:1042	82.2%	1038:1042	82.2%	1038:1042	For neutral pectic polysaccharides, the following results were achieved: BP (enzymatic-assisted extraction, 24 h, 85.9%), PP (nitric acid-assisted extraction, 6 h, 82.2%), and SBP (enzymatic assisted extraction, 48 h, 97.5%; and nitric acid-assisted extraction, 4 h, 83.2%).
26652767	4	63	theme	pectic	1186:1191	arg1	sugars					1193:1198	pectic sugars	1186:1198	pectic sugars	1186:1198	On the basis of the high recovery of pectic sugars, SBP and OH are interesting candidates for the further purification of pectin and production of pectin-derived products.
26652767	4	64	theme	interesting	1216:1226	arg1	SBP					1201:1203	SBP	1201:1203	SBP	1201:1203	On the basis of the high recovery of pectic sugars, SBP and OH are interesting candidates for the further purification of pectin and production of pectin-derived products.
26652767	4	64	theme	interesting	1216:1226	arg1	OH					1209:1210	OH	1209:1210	OH	1209:1210	On the basis of the high recovery of pectic sugars, SBP and OH are interesting candidates for the further purification of pectin and production of pectin-derived products.
26652767	4	64	theme	interesting	1216:1226	arg1	candidates					1228:1237	interesting candidates	1216:1237	interesting candidates for the further purification of pectin and production of pectin-derived products	1216:1318	On the basis of the high recovery of pectic sugars, SBP and OH are interesting candidates for the further purification of pectin and production of pectin-derived products.
26652767	0	65	from	Composition	71:81	arg1	Residues					140:147	Various Agro-Industrial Residues	116:147	Various Agro-Industrial Residues	116:147	Effect of Extraction Conditions on the Saccharide (Neutral and Acidic) Composition of the Crude Pectic Extract from Various Agro-Industrial Residues.
26652767	3	66	theme	nitric	1103:1108	arg1	extraction					1124:1133	nitric acid-assisted extraction	1103:1133	nitric acid-assisted extraction	1103:1133	For neutral pectic polysaccharides, the following results were achieved: BP (enzymatic-assisted extraction, 24 h, 85.9%), PP (nitric acid-assisted extraction, 6 h, 82.2%), and SBP (enzymatic assisted extraction, 48 h, 97.5%; and nitric acid-assisted extraction, 4 h, 83.2%).
26652767	4	67	theme	further	1247:1253	arg1	purification					1255:1266	the further purification	1243:1266	the further purification of pectin and production of pectin-derived products	1243:1318	On the basis of the high recovery of pectic sugars, SBP and OH are interesting candidates for the further purification of pectin and production of pectin-derived products.
26652767	1	68	theme	arabinose	251:259	arg1	galactose					272:280	both neutral (arabinose, rhamnose, galactose) and acidic (galacturonic acid) pectic polysaccharides	237:335	galactose	272:280	The influence of different extraction methodologies was assessed on the composition of both neutral (arabinose, rhamnose, galactose) and acidic (galacturonic acid) pectic polysaccharides obtained from four agro-industrial residues, namely, berry pomace (BP), onion hulls (OH), pressed pumpkin (PP), and sugar beet pulp (SBP).
26652767	1	69	dep	galactose	272:280	arg1	rhamnose					262:269	both neutral (arabinose, rhamnose, galactose) and acidic (galacturonic acid) pectic polysaccharides	237:335	rhamnose	262:269	The influence of different extraction methodologies was assessed on the composition of both neutral (arabinose, rhamnose, galactose) and acidic (galacturonic acid) pectic polysaccharides obtained from four agro-industrial residues, namely, berry pomace (BP), onion hulls (OH), pressed pumpkin (PP), and sugar beet pulp (SBP).
26652767	3	70	theme	acid-assisted	1110:1122	arg1	extraction					1124:1133	nitric acid-assisted extraction	1103:1133	nitric acid-assisted extraction	1103:1133	For neutral pectic polysaccharides, the following results were achieved: BP (enzymatic-assisted extraction, 24 h, 85.9%), PP (nitric acid-assisted extraction, 6 h, 82.2%), and SBP (enzymatic assisted extraction, 48 h, 97.5%; and nitric acid-assisted extraction, 4 h, 83.2%).
26652767	2	71	dep	BP	553:554	arg1	extraction					578:587	nitric acid-assisted extraction	557:587	nitric acid-assisted extraction	557:587	For acidic pectic polysaccharides, the extraction efficiency was obtained as BP (nitric acid-assisted extraction, 2 h, 62.9%), PP (enzymatic-assisted extraction, 12 h, 75.0%), SBP (enzymatic-assisted extraction, 48 h, 89.8%; and nitric acid-assisted extraction, 4 h, 76.5%), and OH (sodium hexametaphosphate-assisted extraction, 0.5 h, 100%; and ammonium oxalate-assisted extraction, 0.5 h, 100%).
26652767	2	71	dep	BP	553:554	arg1	h					592:592	2 h	590:592	2 h	590:592	For acidic pectic polysaccharides, the extraction efficiency was obtained as BP (nitric acid-assisted extraction, 2 h, 62.9%), PP (enzymatic-assisted extraction, 12 h, 75.0%), SBP (enzymatic-assisted extraction, 48 h, 89.8%; and nitric acid-assisted extraction, 4 h, 76.5%), and OH (sodium hexametaphosphate-assisted extraction, 0.5 h, 100%; and ammonium oxalate-assisted extraction, 0.5 h, 100%).
26652767	2	71	dep	BP	553:554	arg1	%					599:599	62.9%	595:599	62.9%	595:599	For acidic pectic polysaccharides, the extraction efficiency was obtained as BP (nitric acid-assisted extraction, 2 h, 62.9%), PP (enzymatic-assisted extraction, 12 h, 75.0%), SBP (enzymatic-assisted extraction, 48 h, 89.8%; and nitric acid-assisted extraction, 4 h, 76.5%), and OH (sodium hexametaphosphate-assisted extraction, 0.5 h, 100%; and ammonium oxalate-assisted extraction, 0.5 h, 100%).
27876596	0	0	theme	promising	83:91	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and immunomodulatory effects of glucans from Pleurotus albidus, a promising species of mushroom for farming and biomass production.
27876596	0	0	theme	promising	83:91	arg1	species					93:99	a promising species	81:99	a promising species of mushroom for farming and biomass production	81:146	Characterization and immunomodulatory effects of glucans from Pleurotus albidus, a promising species of mushroom for farming and biomass production.
27876596	6	1	theme	immunomodulatory	1470:1485	arg1	effects					1487:1493	potential immunomodulatory effects	1460:1493	potential immunomodulatory effects	1460:1493	In conclusion, the basidiome and the submerged culture of P. albidus are sources of easily extractable α- and β-glucans with potential immunomodulatory effects.
27876596	1	2	with	ingredients	233:243	arg1	benefits					267:274	potential health benefits	250:274	potential health benefits	250:274	Polysaccharides from a number of mushroom species are recognized as functional food ingredients with potential health benefits, including immunomodulatory effects.
27876596	1	2	with	ingredients	233:243	arg1	effects					304:310	immunomodulatory effects	287:310	immunomodulatory effects	287:310	Polysaccharides from a number of mushroom species are recognized as functional food ingredients with potential health benefits, including immunomodulatory effects.
27876596	2	3	theme	cold	378:381	arg1	BaCW					390:393	BaCW	390:393	BaCW	390:393	In this study, polysaccharides extracted from the basidiome with cold water (BaCW), hot water (BaHW), and hot alkali (BaHA) solution, and exo- (MyEX) and endopolysaccharides (MyEN) from the submerged culture of Pleurotus albidus, a promising species for farming and biomass production, were analyzed for their chemical composition and structure and immunomodulatory effects on macrophages.
27876596	2	3	theme	cold	378:381	arg1	water					383:387	cold water	378:387	cold water (BaCW)	378:394	In this study, polysaccharides extracted from the basidiome with cold water (BaCW), hot water (BaHW), and hot alkali (BaHA) solution, and exo- (MyEX) and endopolysaccharides (MyEN) from the submerged culture of Pleurotus albidus, a promising species for farming and biomass production, were analyzed for their chemical composition and structure and immunomodulatory effects on macrophages.
27876596	3	4	theme	β-	823:824	arg1	MyEX					815:818	MyEX	815:818	MyEX	815:818	Compositional (HPAEC-PAD and HPSEC-RID/MWD) and structural (FT-IR, 1D- and 2D-NMR) analyses identified BaCW and MyEX as β-(1,6)-branched β-(1,3)-glucans, BaHW and MyEN as α-(1,3)-(1,2)-branched α-(1,6)-glucans, and BaHA as a mixture of α-(1,6)- and β-(1,3)-glucans.
27876596	3	4	theme	β-	823:824	arg1	-glucans					847:854	β-(1,6)-branched β-(1,3)-glucans	823:854	β-(1,6)-branched β-(1,3)-glucans	823:854	Compositional (HPAEC-PAD and HPSEC-RID/MWD) and structural (FT-IR, 1D- and 2D-NMR) analyses identified BaCW and MyEX as β-(1,6)-branched β-(1,3)-glucans, BaHW and MyEN as α-(1,3)-(1,2)-branched α-(1,6)-glucans, and BaHA as a mixture of α-(1,6)- and β-(1,3)-glucans.
27876596	3	4	theme	β-	823:824	arg1	BaCW					806:809	BaCW	806:809	BaCW	806:809	Compositional (HPAEC-PAD and HPSEC-RID/MWD) and structural (FT-IR, 1D- and 2D-NMR) analyses identified BaCW and MyEX as β-(1,6)-branched β-(1,3)-glucans, BaHW and MyEN as α-(1,3)-(1,2)-branched α-(1,6)-glucans, and BaHA as a mixture of α-(1,6)- and β-(1,3)-glucans.
27876596	1	5	theme	health	260:265	arg1	benefits					267:274	potential health benefits	250:274	potential health benefits	250:274	Polysaccharides from a number of mushroom species are recognized as functional food ingredients with potential health benefits, including immunomodulatory effects.
27876596	1	5	theme	health	260:265	arg1	effects					304:310	immunomodulatory effects	287:310	immunomodulatory effects	287:310	Polysaccharides from a number of mushroom species are recognized as functional food ingredients with potential health benefits, including immunomodulatory effects.
27876596	3	6	theme	β-	952:953	arg1	-glucans					959:966	α-(1,6)- and β-(1,3)-glucans	939:966	α-(1,6)- and β-(1,3)-glucans	939:966	Compositional (HPAEC-PAD and HPSEC-RID/MWD) and structural (FT-IR, 1D- and 2D-NMR) analyses identified BaCW and MyEX as β-(1,6)-branched β-(1,3)-glucans, BaHW and MyEN as α-(1,3)-(1,2)-branched α-(1,6)-glucans, and BaHA as a mixture of α-(1,6)- and β-(1,3)-glucans.
27876596	3	7	dep	Compositional	703:715	arg1	HPSEC-RID/MWD					732:744	HPSEC-RID/MWD	732:744	HPSEC-RID/MWD	732:744	Compositional (HPAEC-PAD and HPSEC-RID/MWD) and structural (FT-IR, 1D- and 2D-NMR) analyses identified BaCW and MyEX as β-(1,6)-branched β-(1,3)-glucans, BaHW and MyEN as α-(1,3)-(1,2)-branched α-(1,6)-glucans, and BaHA as a mixture of α-(1,6)- and β-(1,3)-glucans.
27876596	3	7	dep	Compositional	703:715	arg1	HPAEC-PAD					718:726	HPAEC-PAD	718:726	HPAEC-PAD	718:726	Compositional (HPAEC-PAD and HPSEC-RID/MWD) and structural (FT-IR, 1D- and 2D-NMR) analyses identified BaCW and MyEX as β-(1,6)-branched β-(1,3)-glucans, BaHW and MyEN as α-(1,3)-(1,2)-branched α-(1,6)-glucans, and BaHA as a mixture of α-(1,6)- and β-(1,3)-glucans.
27876596	5	8	theme	IL-6	1199:1202	arg1	production					1204:1213	TNF-α, NO and IL-6 production	1185:1213	production	1204:1213	In contrast, BaHW and MyEN induced TNF-α, NO and IL-6 production, and increased zymosan phagocytosis, while BaHA displayed intermediary effects in comparison the other polysaccharides.
27876596	6	9	theme	submerged	1372:1380	arg1	sources					1408:1414	sources	1408:1414	sources of easily extractable α-	1408:1439	In conclusion, the basidiome and the submerged culture of P. albidus are sources of easily extractable α- and β-glucans with potential immunomodulatory effects.
27876596	6	9	theme	submerged	1372:1380	arg1	culture					1382:1388	the submerged culture	1368:1388	the submerged culture of P. albidus	1368:1402	In conclusion, the basidiome and the submerged culture of P. albidus are sources of easily extractable α- and β-glucans with potential immunomodulatory effects.
27876596	6	9	theme	submerged	1372:1380	arg1	basidiome					1354:1362	the basidiome	1350:1362	the basidiome	1350:1362	In conclusion, the basidiome and the submerged culture of P. albidus are sources of easily extractable α- and β-glucans with potential immunomodulatory effects.
27876596	3	10	theme	1,6	900:902	arg1	-glucans					904:911	(1,2)-branched α-(1,6)-glucans	882:911	(1,2)-branched α-(1,6)-glucans	882:911	Compositional (HPAEC-PAD and HPSEC-RID/MWD) and structural (FT-IR, 1D- and 2D-NMR) analyses identified BaCW and MyEX as β-(1,6)-branched β-(1,3)-glucans, BaHW and MyEN as α-(1,3)-(1,2)-branched α-(1,6)-glucans, and BaHA as a mixture of α-(1,6)- and β-(1,3)-glucans.
27876596	4	11	theme	particles	1139:1147	arg1	phagocytosis					1115:1126	phagocytosis	1115:1126	phagocytosis of zymosan particles	1115:1147	BaCW and MyEX stimulated the production of tumor necrosis factor alpha (TNF-α) and nitric oxide (NO), but not interleukin-6 (IL-6), and decreased phagocytosis of zymosan particles.
27876596	5	12	theme	intermediary	1273:1284	arg1	effects					1286:1292	intermediary effects	1273:1292	intermediary effects	1273:1292	In contrast, BaHW and MyEN induced TNF-α, NO and IL-6 production, and increased zymosan phagocytosis, while BaHA displayed intermediary effects in comparison the other polysaccharides.
27876596	0	13	from	Characterization	0:15	arg1	albidus					72:78	Pleurotus albidus	62:78	Pleurotus albidus	62:78	Characterization and immunomodulatory effects of glucans from Pleurotus albidus, a promising species of mushroom for farming and biomass production.
27876596	2	14	theme	albidus	534:540	arg1	culture					513:519	the submerged culture	499:519	the submerged culture of Pleurotus albidus, a promising species for farming and biomass production,	499:597	In this study, polysaccharides extracted from the basidiome with cold water (BaCW), hot water (BaHW), and hot alkali (BaHA) solution, and exo- (MyEX) and endopolysaccharides (MyEN) from the submerged culture of Pleurotus albidus, a promising species for farming and biomass production, were analyzed for their chemical composition and structure and immunomodulatory effects on macrophages.
27876596	4	15	theme	necrosis	1018:1025	arg1	TNF-α					1041:1045	TNF-α	1041:1045	TNF-α	1041:1045	BaCW and MyEX stimulated the production of tumor necrosis factor alpha (TNF-α) and nitric oxide (NO), but not interleukin-6 (IL-6), and decreased phagocytosis of zymosan particles.
27876596	4	15	theme	necrosis	1018:1025	arg1	alpha					1034:1038	tumor necrosis factor alpha	1012:1038	tumor necrosis factor alpha (TNF-α)	1012:1046	BaCW and MyEX stimulated the production of tumor necrosis factor alpha (TNF-α) and nitric oxide (NO), but not interleukin-6 (IL-6), and decreased phagocytosis of zymosan particles.
27876596	2	16	theme	Pleurotus	524:532	arg1	species					555:561	a promising species	543:561	a promising species for farming and biomass production	543:596	In this study, polysaccharides extracted from the basidiome with cold water (BaCW), hot water (BaHW), and hot alkali (BaHA) solution, and exo- (MyEX) and endopolysaccharides (MyEN) from the submerged culture of Pleurotus albidus, a promising species for farming and biomass production, were analyzed for their chemical composition and structure and immunomodulatory effects on macrophages.
27876596	2	16	theme	Pleurotus	524:532	arg1	albidus					534:540	Pleurotus albidus	524:540	Pleurotus albidus	524:540	In this study, polysaccharides extracted from the basidiome with cold water (BaCW), hot water (BaHW), and hot alkali (BaHA) solution, and exo- (MyEX) and endopolysaccharides (MyEN) from the submerged culture of Pleurotus albidus, a promising species for farming and biomass production, were analyzed for their chemical composition and structure and immunomodulatory effects on macrophages.
27876596	2	17	theme	biomass	579:585	arg1	production					587:596	biomass production	579:596	biomass production	579:596	In this study, polysaccharides extracted from the basidiome with cold water (BaCW), hot water (BaHW), and hot alkali (BaHA) solution, and exo- (MyEX) and endopolysaccharides (MyEN) from the submerged culture of Pleurotus albidus, a promising species for farming and biomass production, were analyzed for their chemical composition and structure and immunomodulatory effects on macrophages.
27876596	6	18	with	β-glucans	1445:1453	arg1	effects					1487:1493	potential immunomodulatory effects	1460:1493	potential immunomodulatory effects	1460:1493	In conclusion, the basidiome and the submerged culture of P. albidus are sources of easily extractable α- and β-glucans with potential immunomodulatory effects.
27876596	3	19	theme	-glucans	959:966	arg1	MyEX					815:818	MyEX	815:818	MyEX	815:818	Compositional (HPAEC-PAD and HPSEC-RID/MWD) and structural (FT-IR, 1D- and 2D-NMR) analyses identified BaCW and MyEX as β-(1,6)-branched β-(1,3)-glucans, BaHW and MyEN as α-(1,3)-(1,2)-branched α-(1,6)-glucans, and BaHA as a mixture of α-(1,6)- and β-(1,3)-glucans.
27876596	3	19	theme	-glucans	959:966	arg1	BaCW					806:809	BaCW	806:809	BaCW	806:809	Compositional (HPAEC-PAD and HPSEC-RID/MWD) and structural (FT-IR, 1D- and 2D-NMR) analyses identified BaCW and MyEX as β-(1,6)-branched β-(1,3)-glucans, BaHW and MyEN as α-(1,3)-(1,2)-branched α-(1,6)-glucans, and BaHA as a mixture of α-(1,6)- and β-(1,3)-glucans.
27876596	3	19	theme	-glucans	959:966	arg1	mixture					928:934	a mixture	926:934	a mixture of α-(1,6)- and β-(1,3)-glucans	926:966	Compositional (HPAEC-PAD and HPSEC-RID/MWD) and structural (FT-IR, 1D- and 2D-NMR) analyses identified BaCW and MyEX as β-(1,6)-branched β-(1,3)-glucans, BaHW and MyEN as α-(1,3)-(1,2)-branched α-(1,6)-glucans, and BaHA as a mixture of α-(1,6)- and β-(1,3)-glucans.
27876596	4	20	theme	tumor	1012:1016	arg1	TNF-α					1041:1045	TNF-α	1041:1045	TNF-α	1041:1045	BaCW and MyEX stimulated the production of tumor necrosis factor alpha (TNF-α) and nitric oxide (NO), but not interleukin-6 (IL-6), and decreased phagocytosis of zymosan particles.
27876596	4	20	theme	tumor	1012:1016	arg1	alpha					1034:1038	tumor necrosis factor alpha	1012:1038	tumor necrosis factor alpha (TNF-α)	1012:1046	BaCW and MyEX stimulated the production of tumor necrosis factor alpha (TNF-α) and nitric oxide (NO), but not interleukin-6 (IL-6), and decreased phagocytosis of zymosan particles.
27876596	2	21	from	structure	648:656	arg1	macrophages					690:700	macrophages	690:700	macrophages	690:700	In this study, polysaccharides extracted from the basidiome with cold water (BaCW), hot water (BaHW), and hot alkali (BaHA) solution, and exo- (MyEX) and endopolysaccharides (MyEN) from the submerged culture of Pleurotus albidus, a promising species for farming and biomass production, were analyzed for their chemical composition and structure and immunomodulatory effects on macrophages.
27876596	2	22	theme	hot	397:399	arg1	BaHW					408:411	BaHW	408:411	BaHW	408:411	In this study, polysaccharides extracted from the basidiome with cold water (BaCW), hot water (BaHW), and hot alkali (BaHA) solution, and exo- (MyEX) and endopolysaccharides (MyEN) from the submerged culture of Pleurotus albidus, a promising species for farming and biomass production, were analyzed for their chemical composition and structure and immunomodulatory effects on macrophages.
27876596	2	22	theme	hot	397:399	arg1	water					401:405	hot water	397:405	hot water (BaHW)	397:412	In this study, polysaccharides extracted from the basidiome with cold water (BaCW), hot water (BaHW), and hot alkali (BaHA) solution, and exo- (MyEX) and endopolysaccharides (MyEN) from the submerged culture of Pleurotus albidus, a promising species for farming and biomass production, were analyzed for their chemical composition and structure and immunomodulatory effects on macrophages.
27876596	0	23	theme	mushroom	104:111	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and immunomodulatory effects of glucans from Pleurotus albidus, a promising species of mushroom for farming and biomass production.
27876596	0	23	theme	mushroom	104:111	arg1	species					93:99	a promising species	81:99	a promising species of mushroom for farming and biomass production	81:146	Characterization and immunomodulatory effects of glucans from Pleurotus albidus, a promising species of mushroom for farming and biomass production.
27876596	6	24	theme	α-	1438:1439	arg1	sources					1408:1414	sources	1408:1414	sources of easily extractable α-	1408:1439	In conclusion, the basidiome and the submerged culture of P. albidus are sources of easily extractable α- and β-glucans with potential immunomodulatory effects.
27876596	6	24	theme	α-	1438:1439	arg1	culture					1382:1388	the submerged culture	1368:1388	the submerged culture of P. albidus	1368:1402	In conclusion, the basidiome and the submerged culture of P. albidus are sources of easily extractable α- and β-glucans with potential immunomodulatory effects.
27876596	6	24	theme	α-	1438:1439	arg1	β-glucans					1445:1453	β-glucans	1445:1453	β-glucans with potential immunomodulatory effects	1445:1493	In conclusion, the basidiome and the submerged culture of P. albidus are sources of easily extractable α- and β-glucans with potential immunomodulatory effects.
27876596	6	24	theme	α-	1438:1439	arg1	basidiome					1354:1362	the basidiome	1350:1362	the basidiome	1350:1362	In conclusion, the basidiome and the submerged culture of P. albidus are sources of easily extractable α- and β-glucans with potential immunomodulatory effects.
27876596	5	25	theme	TNF-α	1185:1189	arg1	production					1204:1213	TNF-α, NO and IL-6 production	1185:1213	production	1204:1213	In contrast, BaHW and MyEN induced TNF-α, NO and IL-6 production, and increased zymosan phagocytosis, while BaHA displayed intermediary effects in comparison the other polysaccharides.
27876596	3	26	theme	-branched	887:895	arg1	-glucans					904:911	(1,2)-branched α-(1,6)-glucans	882:911	(1,2)-branched α-(1,6)-glucans	882:911	Compositional (HPAEC-PAD and HPSEC-RID/MWD) and structural (FT-IR, 1D- and 2D-NMR) analyses identified BaCW and MyEX as β-(1,6)-branched β-(1,3)-glucans, BaHW and MyEN as α-(1,3)-(1,2)-branched α-(1,6)-glucans, and BaHA as a mixture of α-(1,6)- and β-(1,3)-glucans.
27876596	6	27	from	β-glucans	1445:1453	arg1	conclusion					1338:1347	conclusion	1338:1347	conclusion	1338:1347	In conclusion, the basidiome and the submerged culture of P. albidus are sources of easily extractable α- and β-glucans with potential immunomodulatory effects.
27876596	2	28	theme	alkali	423:428	arg1	solution					437:444	hot alkali (BaHA) solution	419:444	hot alkali (BaHA) solution	419:444	In this study, polysaccharides extracted from the basidiome with cold water (BaCW), hot water (BaHW), and hot alkali (BaHA) solution, and exo- (MyEX) and endopolysaccharides (MyEN) from the submerged culture of Pleurotus albidus, a promising species for farming and biomass production, were analyzed for their chemical composition and structure and immunomodulatory effects on macrophages.
27876596	6	29	theme	extractable	1426:1436	arg1	α-					1438:1439	easily extractable α-	1419:1439	easily extractable α-	1419:1439	In conclusion, the basidiome and the submerged culture of P. albidus are sources of easily extractable α- and β-glucans with potential immunomodulatory effects.
27876596	3	30	theme	α-	897:898	arg1	-glucans					904:911	(1,2)-branched α-(1,6)-glucans	882:911	(1,2)-branched α-(1,6)-glucans	882:911	Compositional (HPAEC-PAD and HPSEC-RID/MWD) and structural (FT-IR, 1D- and 2D-NMR) analyses identified BaCW and MyEX as β-(1,6)-branched β-(1,3)-glucans, BaHW and MyEN as α-(1,3)-(1,2)-branched α-(1,6)-glucans, and BaHA as a mixture of α-(1,6)- and β-(1,3)-glucans.
27876596	6	31	from	sources	1408:1414	arg1	conclusion					1338:1347	conclusion	1338:1347	conclusion	1338:1347	In conclusion, the basidiome and the submerged culture of P. albidus are sources of easily extractable α- and β-glucans with potential immunomodulatory effects.
27876596	3	32	theme	1,6	826:828	arg1	MyEX					815:818	MyEX	815:818	MyEX	815:818	Compositional (HPAEC-PAD and HPSEC-RID/MWD) and structural (FT-IR, 1D- and 2D-NMR) analyses identified BaCW and MyEX as β-(1,6)-branched β-(1,3)-glucans, BaHW and MyEN as α-(1,3)-(1,2)-branched α-(1,6)-glucans, and BaHA as a mixture of α-(1,6)- and β-(1,3)-glucans.
27876596	3	32	theme	1,6	826:828	arg1	-glucans					847:854	β-(1,6)-branched β-(1,3)-glucans	823:854	β-(1,6)-branched β-(1,3)-glucans	823:854	Compositional (HPAEC-PAD and HPSEC-RID/MWD) and structural (FT-IR, 1D- and 2D-NMR) analyses identified BaCW and MyEX as β-(1,6)-branched β-(1,3)-glucans, BaHW and MyEN as α-(1,3)-(1,2)-branched α-(1,6)-glucans, and BaHA as a mixture of α-(1,6)- and β-(1,3)-glucans.
27876596	3	32	theme	1,6	826:828	arg1	BaCW					806:809	BaCW	806:809	BaCW	806:809	Compositional (HPAEC-PAD and HPSEC-RID/MWD) and structural (FT-IR, 1D- and 2D-NMR) analyses identified BaCW and MyEX as β-(1,6)-branched β-(1,3)-glucans, BaHW and MyEN as α-(1,3)-(1,2)-branched α-(1,6)-glucans, and BaHA as a mixture of α-(1,6)- and β-(1,3)-glucans.
27876596	1	33	theme	mushroom	182:189	arg1	species					191:197	mushroom species	182:197	mushroom species	182:197	Polysaccharides from a number of mushroom species are recognized as functional food ingredients with potential health benefits, including immunomodulatory effects.
27876596	2	34	theme	hot	419:421	arg1	solution					437:444	hot alkali (BaHA) solution	419:444	hot alkali (BaHA) solution	419:444	In this study, polysaccharides extracted from the basidiome with cold water (BaCW), hot water (BaHW), and hot alkali (BaHA) solution, and exo- (MyEX) and endopolysaccharides (MyEN) from the submerged culture of Pleurotus albidus, a promising species for farming and biomass production, were analyzed for their chemical composition and structure and immunomodulatory effects on macrophages.
27876596	1	35	theme	immunomodulatory	287:302	arg1	effects					304:310	immunomodulatory effects	287:310	immunomodulatory effects	287:310	Polysaccharides from a number of mushroom species are recognized as functional food ingredients with potential health benefits, including immunomodulatory effects.
27876596	2	36	theme	promising	545:553	arg1	species					555:561	a promising species	543:561	a promising species for farming and biomass production	543:596	In this study, polysaccharides extracted from the basidiome with cold water (BaCW), hot water (BaHW), and hot alkali (BaHA) solution, and exo- (MyEX) and endopolysaccharides (MyEN) from the submerged culture of Pleurotus albidus, a promising species for farming and biomass production, were analyzed for their chemical composition and structure and immunomodulatory effects on macrophages.
27876596	2	36	theme	promising	545:553	arg1	albidus					534:540	Pleurotus albidus	524:540	Pleurotus albidus	524:540	In this study, polysaccharides extracted from the basidiome with cold water (BaCW), hot water (BaHW), and hot alkali (BaHA) solution, and exo- (MyEX) and endopolysaccharides (MyEN) from the submerged culture of Pleurotus albidus, a promising species for farming and biomass production, were analyzed for their chemical composition and structure and immunomodulatory effects on macrophages.
27876596	3	37	theme	1,2	883:885	arg1	-glucans					904:911	(1,2)-branched α-(1,6)-glucans	882:911	(1,2)-branched α-(1,6)-glucans	882:911	Compositional (HPAEC-PAD and HPSEC-RID/MWD) and structural (FT-IR, 1D- and 2D-NMR) analyses identified BaCW and MyEX as β-(1,6)-branched β-(1,3)-glucans, BaHW and MyEN as α-(1,3)-(1,2)-branched α-(1,6)-glucans, and BaHA as a mixture of α-(1,6)- and β-(1,3)-glucans.
27876596	6	38	theme	albidus	1396:1402	arg1	sources					1408:1414	sources	1408:1414	sources of easily extractable α-	1408:1439	In conclusion, the basidiome and the submerged culture of P. albidus are sources of easily extractable α- and β-glucans with potential immunomodulatory effects.
27876596	6	38	theme	albidus	1396:1402	arg1	culture					1382:1388	the submerged culture	1368:1388	the submerged culture of P. albidus	1368:1402	In conclusion, the basidiome and the submerged culture of P. albidus are sources of easily extractable α- and β-glucans with potential immunomodulatory effects.
27876596	6	38	theme	albidus	1396:1402	arg1	basidiome					1354:1362	the basidiome	1350:1362	the basidiome	1350:1362	In conclusion, the basidiome and the submerged culture of P. albidus are sources of easily extractable α- and β-glucans with potential immunomodulatory effects.
27876596	3	39	theme	1,3	843:845	arg1	MyEX					815:818	MyEX	815:818	MyEX	815:818	Compositional (HPAEC-PAD and HPSEC-RID/MWD) and structural (FT-IR, 1D- and 2D-NMR) analyses identified BaCW and MyEX as β-(1,6)-branched β-(1,3)-glucans, BaHW and MyEN as α-(1,3)-(1,2)-branched α-(1,6)-glucans, and BaHA as a mixture of α-(1,6)- and β-(1,3)-glucans.
27876596	3	39	theme	1,3	843:845	arg1	-glucans					847:854	β-(1,6)-branched β-(1,3)-glucans	823:854	β-(1,6)-branched β-(1,3)-glucans	823:854	Compositional (HPAEC-PAD and HPSEC-RID/MWD) and structural (FT-IR, 1D- and 2D-NMR) analyses identified BaCW and MyEX as β-(1,6)-branched β-(1,3)-glucans, BaHW and MyEN as α-(1,3)-(1,2)-branched α-(1,6)-glucans, and BaHA as a mixture of α-(1,6)- and β-(1,3)-glucans.
27876596	3	39	theme	1,3	843:845	arg1	BaCW					806:809	BaCW	806:809	BaCW	806:809	Compositional (HPAEC-PAD and HPSEC-RID/MWD) and structural (FT-IR, 1D- and 2D-NMR) analyses identified BaCW and MyEX as β-(1,6)-branched β-(1,3)-glucans, BaHW and MyEN as α-(1,3)-(1,2)-branched α-(1,6)-glucans, and BaHA as a mixture of α-(1,6)- and β-(1,3)-glucans.
27876596	1	40	theme	species	191:197	arg1	number					172:177	a number	170:177	a number of mushroom species	170:197	Polysaccharides from a number of mushroom species are recognized as functional food ingredients with potential health benefits, including immunomodulatory effects.
27876596	0	41	theme	immunomodulatory	21:36	arg1	effects					38:44	immunomodulatory effects	21:44	immunomodulatory effects	21:44	Characterization and immunomodulatory effects of glucans from Pleurotus albidus, a promising species of mushroom for farming and biomass production.
27876596	4	42	theme	zymosan	1131:1137	arg1	particles					1139:1147	zymosan particles	1131:1147	zymosan particles	1131:1147	BaCW and MyEX stimulated the production of tumor necrosis factor alpha (TNF-α) and nitric oxide (NO), but not interleukin-6 (IL-6), and decreased phagocytosis of zymosan particles.
27876596	3	43	theme	α-	939:940	arg1	-glucans					959:966	α-(1,6)- and β-(1,3)-glucans	939:966	α-(1,6)- and β-(1,3)-glucans	939:966	Compositional (HPAEC-PAD and HPSEC-RID/MWD) and structural (FT-IR, 1D- and 2D-NMR) analyses identified BaCW and MyEX as β-(1,6)-branched β-(1,3)-glucans, BaHW and MyEN as α-(1,3)-(1,2)-branched α-(1,6)-glucans, and BaHA as a mixture of α-(1,6)- and β-(1,3)-glucans.
27876596	6	44	theme	potential	1460:1468	arg1	effects					1487:1493	potential immunomodulatory effects	1460:1493	potential immunomodulatory effects	1460:1493	In conclusion, the basidiome and the submerged culture of P. albidus are sources of easily extractable α- and β-glucans with potential immunomodulatory effects.
27876596	3	45	theme	β-	840:841	arg1	MyEX					815:818	MyEX	815:818	MyEX	815:818	Compositional (HPAEC-PAD and HPSEC-RID/MWD) and structural (FT-IR, 1D- and 2D-NMR) analyses identified BaCW and MyEX as β-(1,6)-branched β-(1,3)-glucans, BaHW and MyEN as α-(1,3)-(1,2)-branched α-(1,6)-glucans, and BaHA as a mixture of α-(1,6)- and β-(1,3)-glucans.
27876596	3	45	theme	β-	840:841	arg1	-glucans					847:854	β-(1,6)-branched β-(1,3)-glucans	823:854	β-(1,6)-branched β-(1,3)-glucans	823:854	Compositional (HPAEC-PAD and HPSEC-RID/MWD) and structural (FT-IR, 1D- and 2D-NMR) analyses identified BaCW and MyEX as β-(1,6)-branched β-(1,3)-glucans, BaHW and MyEN as α-(1,3)-(1,2)-branched α-(1,6)-glucans, and BaHA as a mixture of α-(1,6)- and β-(1,3)-glucans.
27876596	3	45	theme	β-	840:841	arg1	BaCW					806:809	BaCW	806:809	BaCW	806:809	Compositional (HPAEC-PAD and HPSEC-RID/MWD) and structural (FT-IR, 1D- and 2D-NMR) analyses identified BaCW and MyEX as β-(1,6)-branched β-(1,3)-glucans, BaHW and MyEN as α-(1,3)-(1,2)-branched α-(1,6)-glucans, and BaHA as a mixture of α-(1,6)- and β-(1,3)-glucans.
27876596	0	46	theme	glucans	49:55	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and immunomodulatory effects of glucans from Pleurotus albidus, a promising species of mushroom for farming and biomass production.
27876596	0	46	theme	glucans	49:55	arg1	effects					38:44	immunomodulatory effects	21:44	immunomodulatory effects	21:44	Characterization and immunomodulatory effects of glucans from Pleurotus albidus, a promising species of mushroom for farming and biomass production.
27876596	0	46	theme	glucans	49:55	arg1	species					93:99	a promising species	81:99	a promising species of mushroom for farming and biomass production	81:146	Characterization and immunomodulatory effects of glucans from Pleurotus albidus, a promising species of mushroom for farming and biomass production.
27876596	3	47	theme	-branched	830:838	arg1	MyEX					815:818	MyEX	815:818	MyEX	815:818	Compositional (HPAEC-PAD and HPSEC-RID/MWD) and structural (FT-IR, 1D- and 2D-NMR) analyses identified BaCW and MyEX as β-(1,6)-branched β-(1,3)-glucans, BaHW and MyEN as α-(1,3)-(1,2)-branched α-(1,6)-glucans, and BaHA as a mixture of α-(1,6)- and β-(1,3)-glucans.
27876596	3	47	theme	-branched	830:838	arg1	-glucans					847:854	β-(1,6)-branched β-(1,3)-glucans	823:854	β-(1,6)-branched β-(1,3)-glucans	823:854	Compositional (HPAEC-PAD and HPSEC-RID/MWD) and structural (FT-IR, 1D- and 2D-NMR) analyses identified BaCW and MyEX as β-(1,6)-branched β-(1,3)-glucans, BaHW and MyEN as α-(1,3)-(1,2)-branched α-(1,6)-glucans, and BaHA as a mixture of α-(1,6)- and β-(1,3)-glucans.
27876596	3	47	theme	-branched	830:838	arg1	BaCW					806:809	BaCW	806:809	BaCW	806:809	Compositional (HPAEC-PAD and HPSEC-RID/MWD) and structural (FT-IR, 1D- and 2D-NMR) analyses identified BaCW and MyEX as β-(1,6)-branched β-(1,3)-glucans, BaHW and MyEN as α-(1,3)-(1,2)-branched α-(1,6)-glucans, and BaHA as a mixture of α-(1,6)- and β-(1,3)-glucans.
27876596	3	48	dep	structural	751:760	arg1	1D-					770:772	1D-	770:772	1D-	770:772	Compositional (HPAEC-PAD and HPSEC-RID/MWD) and structural (FT-IR, 1D- and 2D-NMR) analyses identified BaCW and MyEX as β-(1,6)-branched β-(1,3)-glucans, BaHW and MyEN as α-(1,3)-(1,2)-branched α-(1,6)-glucans, and BaHA as a mixture of α-(1,6)- and β-(1,3)-glucans.
27876596	3	48	dep	structural	751:760	arg1	FT-IR					763:767	FT-IR	763:767	FT-IR	763:767	Compositional (HPAEC-PAD and HPSEC-RID/MWD) and structural (FT-IR, 1D- and 2D-NMR) analyses identified BaCW and MyEX as β-(1,6)-branched β-(1,3)-glucans, BaHW and MyEN as α-(1,3)-(1,2)-branched α-(1,6)-glucans, and BaHA as a mixture of α-(1,6)- and β-(1,3)-glucans.
27876596	3	48	dep	structural	751:760	arg1	2D-NMR					778:783	2D-NMR	778:783	2D-NMR	778:783	Compositional (HPAEC-PAD and HPSEC-RID/MWD) and structural (FT-IR, 1D- and 2D-NMR) analyses identified BaCW and MyEX as β-(1,6)-branched β-(1,3)-glucans, BaHW and MyEN as α-(1,3)-(1,2)-branched α-(1,6)-glucans, and BaHA as a mixture of α-(1,6)- and β-(1,3)-glucans.
27876596	2	49	from	composition	632:642	arg1	macrophages					690:700	macrophages	690:700	macrophages	690:700	In this study, polysaccharides extracted from the basidiome with cold water (BaCW), hot water (BaHW), and hot alkali (BaHA) solution, and exo- (MyEX) and endopolysaccharides (MyEN) from the submerged culture of Pleurotus albidus, a promising species for farming and biomass production, were analyzed for their chemical composition and structure and immunomodulatory effects on macrophages.
27876596	0	50	theme	biomass	129:135	arg1	production					137:146	biomass production	129:146	biomass production	129:146	Characterization and immunomodulatory effects of glucans from Pleurotus albidus, a promising species of mushroom for farming and biomass production.
27876596	2	51	with	basidiome	363:371	arg1	endopolysaccharides					467:485	endopolysaccharides	467:485	endopolysaccharides (MyEN)	467:492	In this study, polysaccharides extracted from the basidiome with cold water (BaCW), hot water (BaHW), and hot alkali (BaHA) solution, and exo- (MyEX) and endopolysaccharides (MyEN) from the submerged culture of Pleurotus albidus, a promising species for farming and biomass production, were analyzed for their chemical composition and structure and immunomodulatory effects on macrophages.
27876596	2	51	with	basidiome	363:371	arg1	water					383:387	cold water	378:387	cold water (BaCW)	378:394	In this study, polysaccharides extracted from the basidiome with cold water (BaCW), hot water (BaHW), and hot alkali (BaHA) solution, and exo- (MyEX) and endopolysaccharides (MyEN) from the submerged culture of Pleurotus albidus, a promising species for farming and biomass production, were analyzed for their chemical composition and structure and immunomodulatory effects on macrophages.
27876596	2	51	with	basidiome	363:371	arg1	water					401:405	hot water	397:405	hot water (BaHW)	397:412	In this study, polysaccharides extracted from the basidiome with cold water (BaCW), hot water (BaHW), and hot alkali (BaHA) solution, and exo- (MyEX) and endopolysaccharides (MyEN) from the submerged culture of Pleurotus albidus, a promising species for farming and biomass production, were analyzed for their chemical composition and structure and immunomodulatory effects on macrophages.
27876596	2	51	with	basidiome	363:371	arg1	exo-					451:454	exo-	451:454	exo- (MyEX)	451:461	In this study, polysaccharides extracted from the basidiome with cold water (BaCW), hot water (BaHW), and hot alkali (BaHA) solution, and exo- (MyEX) and endopolysaccharides (MyEN) from the submerged culture of Pleurotus albidus, a promising species for farming and biomass production, were analyzed for their chemical composition and structure and immunomodulatory effects on macrophages.
27876596	2	51	with	basidiome	363:371	arg1	BaHW					408:411	BaHW	408:411	BaHW	408:411	In this study, polysaccharides extracted from the basidiome with cold water (BaCW), hot water (BaHW), and hot alkali (BaHA) solution, and exo- (MyEX) and endopolysaccharides (MyEN) from the submerged culture of Pleurotus albidus, a promising species for farming and biomass production, were analyzed for their chemical composition and structure and immunomodulatory effects on macrophages.
27876596	2	51	with	basidiome	363:371	arg1	MyEN					488:491	MyEN	488:491	MyEN	488:491	In this study, polysaccharides extracted from the basidiome with cold water (BaCW), hot water (BaHW), and hot alkali (BaHA) solution, and exo- (MyEX) and endopolysaccharides (MyEN) from the submerged culture of Pleurotus albidus, a promising species for farming and biomass production, were analyzed for their chemical composition and structure and immunomodulatory effects on macrophages.
27876596	2	51	with	basidiome	363:371	arg1	BaCW					390:393	BaCW	390:393	BaCW	390:393	In this study, polysaccharides extracted from the basidiome with cold water (BaCW), hot water (BaHW), and hot alkali (BaHA) solution, and exo- (MyEX) and endopolysaccharides (MyEN) from the submerged culture of Pleurotus albidus, a promising species for farming and biomass production, were analyzed for their chemical composition and structure and immunomodulatory effects on macrophages.
27876596	2	51	with	basidiome	363:371	arg1	MyEX					457:460	MyEX	457:460	MyEX	457:460	In this study, polysaccharides extracted from the basidiome with cold water (BaCW), hot water (BaHW), and hot alkali (BaHA) solution, and exo- (MyEX) and endopolysaccharides (MyEN) from the submerged culture of Pleurotus albidus, a promising species for farming and biomass production, were analyzed for their chemical composition and structure and immunomodulatory effects on macrophages.
27876596	2	51	with	basidiome	363:371	arg1	solution					437:444	hot alkali (BaHA) solution	419:444	hot alkali (BaHA) solution	419:444	In this study, polysaccharides extracted from the basidiome with cold water (BaCW), hot water (BaHW), and hot alkali (BaHA) solution, and exo- (MyEX) and endopolysaccharides (MyEN) from the submerged culture of Pleurotus albidus, a promising species for farming and biomass production, were analyzed for their chemical composition and structure and immunomodulatory effects on macrophages.
27876596	1	52	theme	functional	217:226	arg1	Polysaccharides					149:163	Polysaccharides	149:163	Polysaccharides from a number of mushroom species	149:197	Polysaccharides from a number of mushroom species are recognized as functional food ingredients with potential health benefits, including immunomodulatory effects.
27876596	1	52	theme	functional	217:226	arg1	ingredients					233:243	functional food ingredients	217:243	functional food ingredients with potential health benefits, including immunomodulatory effects	217:310	Polysaccharides from a number of mushroom species are recognized as functional food ingredients with potential health benefits, including immunomodulatory effects.
27876596	0	53	theme	Pleurotus	62:70	arg1	albidus					72:78	Pleurotus albidus	62:78	Pleurotus albidus	62:78	Characterization and immunomodulatory effects of glucans from Pleurotus albidus, a promising species of mushroom for farming and biomass production.
27876596	5	54	theme	other	1312:1316	arg1	polysaccharides					1318:1332	the other polysaccharides	1308:1332	comparison the other polysaccharides	1297:1332	In contrast, BaHW and MyEN induced TNF-α, NO and IL-6 production, and increased zymosan phagocytosis, while BaHA displayed intermediary effects in comparison the other polysaccharides.
27876596	0	55	from	albidus	72:78	arg1	glucans					49:55	glucans	49:55	glucans from Pleurotus albidus	49:78	Characterization and immunomodulatory effects of glucans from Pleurotus albidus, a promising species of mushroom for farming and biomass production.
27876596	0	55	from	albidus	72:78	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and immunomodulatory effects of glucans from Pleurotus albidus, a promising species of mushroom for farming and biomass production.
27876596	0	55	from	albidus	72:78	arg1	effects					38:44	immunomodulatory effects	21:44	immunomodulatory effects	21:44	Characterization and immunomodulatory effects of glucans from Pleurotus albidus, a promising species of mushroom for farming and biomass production.
27876596	0	55	from	albidus	72:78	arg1	species					93:99	a promising species	81:99	a promising species of mushroom for farming and biomass production	81:146	Characterization and immunomodulatory effects of glucans from Pleurotus albidus, a promising species of mushroom for farming and biomass production.
27876596	5	56	theme	zymosan	1230:1236	arg1	phagocytosis					1238:1249	zymosan phagocytosis	1230:1249	zymosan phagocytosis	1230:1249	In contrast, BaHW and MyEN induced TNF-α, NO and IL-6 production, and increased zymosan phagocytosis, while BaHA displayed intermediary effects in comparison the other polysaccharides.
27876596	4	57	theme	interleukin-6	1079:1091	arg1	production					998:1007	the production	994:1007	the production of tumor necrosis factor alpha (TNF-α) and nitric oxide (NO), but not interleukin-6 (IL-6),	994:1099	BaCW and MyEX stimulated the production of tumor necrosis factor alpha (TNF-α) and nitric oxide (NO), but not interleukin-6 (IL-6), and decreased phagocytosis of zymosan particles.
27876596	5	58	dep	comparison	1297:1306	arg1	polysaccharides					1318:1332	the other polysaccharides	1308:1332	comparison the other polysaccharides	1297:1332	In contrast, BaHW and MyEN induced TNF-α, NO and IL-6 production, and increased zymosan phagocytosis, while BaHA displayed intermediary effects in comparison the other polysaccharides.
27876596	4	59	theme	alpha	1034:1038	arg1	production					998:1007	the production	994:1007	the production of tumor necrosis factor alpha (TNF-α) and nitric oxide (NO), but not interleukin-6 (IL-6),	994:1099	BaCW and MyEX stimulated the production of tumor necrosis factor alpha (TNF-α) and nitric oxide (NO), but not interleukin-6 (IL-6), and decreased phagocytosis of zymosan particles.
27876596	6	60	theme	P.	1393:1394	arg1	albidus					1396:1402	P. albidus	1393:1402	P. albidus	1393:1402	In conclusion, the basidiome and the submerged culture of P. albidus are sources of easily extractable α- and β-glucans with potential immunomodulatory effects.
27876596	4	61	theme	oxide	1059:1063	arg1	production					998:1007	the production	994:1007	the production of tumor necrosis factor alpha (TNF-α) and nitric oxide (NO), but not interleukin-6 (IL-6),	994:1099	BaCW and MyEX stimulated the production of tumor necrosis factor alpha (TNF-α) and nitric oxide (NO), but not interleukin-6 (IL-6), and decreased phagocytosis of zymosan particles.
27876596	6	62	with	sources	1408:1414	arg1	effects					1487:1493	potential immunomodulatory effects	1460:1493	potential immunomodulatory effects	1460:1493	In conclusion, the basidiome and the submerged culture of P. albidus are sources of easily extractable α- and β-glucans with potential immunomodulatory effects.
27876596	2	63	theme	submerged	503:511	arg1	culture					513:519	the submerged culture	499:519	the submerged culture of Pleurotus albidus, a promising species for farming and biomass production,	499:597	In this study, polysaccharides extracted from the basidiome with cold water (BaCW), hot water (BaHW), and hot alkali (BaHA) solution, and exo- (MyEX) and endopolysaccharides (MyEN) from the submerged culture of Pleurotus albidus, a promising species for farming and biomass production, were analyzed for their chemical composition and structure and immunomodulatory effects on macrophages.
27876596	4	64	theme	factor	1027:1032	arg1	TNF-α					1041:1045	TNF-α	1041:1045	TNF-α	1041:1045	BaCW and MyEX stimulated the production of tumor necrosis factor alpha (TNF-α) and nitric oxide (NO), but not interleukin-6 (IL-6), and decreased phagocytosis of zymosan particles.
27876596	4	64	theme	factor	1027:1032	arg1	alpha					1034:1038	tumor necrosis factor alpha	1012:1038	tumor necrosis factor alpha (TNF-α)	1012:1046	BaCW and MyEX stimulated the production of tumor necrosis factor alpha (TNF-α) and nitric oxide (NO), but not interleukin-6 (IL-6), and decreased phagocytosis of zymosan particles.
27876596	2	65	theme	chemical	623:630	arg1	composition					632:642	their chemical composition	617:642	their chemical composition	617:642	In this study, polysaccharides extracted from the basidiome with cold water (BaCW), hot water (BaHW), and hot alkali (BaHA) solution, and exo- (MyEX) and endopolysaccharides (MyEN) from the submerged culture of Pleurotus albidus, a promising species for farming and biomass production, were analyzed for their chemical composition and structure and immunomodulatory effects on macrophages.
27876596	2	66	theme	immunomodulatory	662:677	arg1	effects					679:685	immunomodulatory effects	662:685	immunomodulatory effects on macrophages	662:700	In this study, polysaccharides extracted from the basidiome with cold water (BaCW), hot water (BaHW), and hot alkali (BaHA) solution, and exo- (MyEX) and endopolysaccharides (MyEN) from the submerged culture of Pleurotus albidus, a promising species for farming and biomass production, were analyzed for their chemical composition and structure and immunomodulatory effects on macrophages.
27876596	1	67	theme	food	228:231	arg1	Polysaccharides					149:163	Polysaccharides	149:163	Polysaccharides from a number of mushroom species	149:197	Polysaccharides from a number of mushroom species are recognized as functional food ingredients with potential health benefits, including immunomodulatory effects.
27876596	1	67	theme	food	228:231	arg1	ingredients					233:243	functional food ingredients	217:243	functional food ingredients with potential health benefits, including immunomodulatory effects	217:310	Polysaccharides from a number of mushroom species are recognized as functional food ingredients with potential health benefits, including immunomodulatory effects.
27876596	2	68	from	effects	679:685	arg1	macrophages					690:700	macrophages	690:700	macrophages	690:700	In this study, polysaccharides extracted from the basidiome with cold water (BaCW), hot water (BaHW), and hot alkali (BaHA) solution, and exo- (MyEX) and endopolysaccharides (MyEN) from the submerged culture of Pleurotus albidus, a promising species for farming and biomass production, were analyzed for their chemical composition and structure and immunomodulatory effects on macrophages.
27876596	2	69	theme	BaHA	431:434	arg1	solution					437:444	hot alkali (BaHA) solution	419:444	hot alkali (BaHA) solution	419:444	In this study, polysaccharides extracted from the basidiome with cold water (BaCW), hot water (BaHW), and hot alkali (BaHA) solution, and exo- (MyEX) and endopolysaccharides (MyEN) from the submerged culture of Pleurotus albidus, a promising species for farming and biomass production, were analyzed for their chemical composition and structure and immunomodulatory effects on macrophages.
27876596	0	70	from	effects	38:44	arg1	albidus					72:78	Pleurotus albidus	62:78	Pleurotus albidus	62:78	Characterization and immunomodulatory effects of glucans from Pleurotus albidus, a promising species of mushroom for farming and biomass production.
27876596	3	71	theme	structural	751:760	arg1	analyses					786:793	structural (FT-IR, 1D- and 2D-NMR) analyses	751:793	structural (FT-IR, 1D- and 2D-NMR) analyses	751:793	Compositional (HPAEC-PAD and HPSEC-RID/MWD) and structural (FT-IR, 1D- and 2D-NMR) analyses identified BaCW and MyEX as β-(1,6)-branched β-(1,3)-glucans, BaHW and MyEN as α-(1,3)-(1,2)-branched α-(1,6)-glucans, and BaHA as a mixture of α-(1,6)- and β-(1,3)-glucans.
27876596	1	72	from	number	172:177	arg1	Polysaccharides					149:163	Polysaccharides	149:163	Polysaccharides from a number of mushroom species	149:197	Polysaccharides from a number of mushroom species are recognized as functional food ingredients with potential health benefits, including immunomodulatory effects.
27876596	1	72	from	number	172:177	arg1	ingredients					233:243	functional food ingredients	217:243	functional food ingredients with potential health benefits, including immunomodulatory effects	217:310	Polysaccharides from a number of mushroom species are recognized as functional food ingredients with potential health benefits, including immunomodulatory effects.
27876596	5	73	theme	NO	1192:1193	arg1	production					1204:1213	TNF-α, NO and IL-6 production	1185:1213	production	1204:1213	In contrast, BaHW and MyEN induced TNF-α, NO and IL-6 production, and increased zymosan phagocytosis, while BaHA displayed intermediary effects in comparison the other polysaccharides.
27876596	4	74	theme	nitric	1052:1057	arg1	NO					1066:1067	NO	1066:1067	NO	1066:1067	BaCW and MyEX stimulated the production of tumor necrosis factor alpha (TNF-α) and nitric oxide (NO), but not interleukin-6 (IL-6), and decreased phagocytosis of zymosan particles.
27876596	4	74	theme	nitric	1052:1057	arg1	oxide					1059:1063	nitric oxide	1052:1063	nitric oxide (NO)	1052:1068	BaCW and MyEX stimulated the production of tumor necrosis factor alpha (TNF-α) and nitric oxide (NO), but not interleukin-6 (IL-6), and decreased phagocytosis of zymosan particles.
27876596	1	75	theme	potential	250:258	arg1	benefits					267:274	potential health benefits	250:274	potential health benefits	250:274	Polysaccharides from a number of mushroom species are recognized as functional food ingredients with potential health benefits, including immunomodulatory effects.
27876596	1	75	theme	potential	250:258	arg1	effects					304:310	immunomodulatory effects	287:310	immunomodulatory effects	287:310	Polysaccharides from a number of mushroom species are recognized as functional food ingredients with potential health benefits, including immunomodulatory effects.
26113633	0	0	theme	algal	76:80	arg1	composition					6:16	Sugar composition	0:16	Sugar composition of the pectic polysaccharides of charophytes	0:61	Sugar composition of the pectic polysaccharides of charophytes, the closest algal relatives of land-plants: presence of 3-O-methyl-D-galactose residues.
26113633	0	0	theme	algal	76:80	arg1	relatives					82:90	the closest algal relatives	64:90	the closest algal relatives of land-plants	64:105	Sugar composition of the pectic polysaccharides of charophytes, the closest algal relatives of land-plants: presence of 3-O-methyl-D-galactose residues.
26113633	5	1	theme	magnetic	1059:1066	arg1	spectroscopy					1078:1089	(1)H/(13)C-nuclear magnetic resonance spectroscopy	1040:1089	(1)H/(13)C-nuclear magnetic resonance spectroscopy	1040:1089	One unusual monosaccharide, 'U', was characterized by (1)H/(13)C-nuclear magnetic resonance spectroscopy and also enzymically.
26113633	14	2	theme	major	2337:2341	arg1	grades					2356:2361	major phylogenetic grades	2337:2361	major phylogenetic grades	2337:2361	The presence of 3-MeGal in charophytes and lycophytes but not in the 'intervening' bryophytes confirms that cell-wall chemistry changed drastically between major phylogenetic grades.
26113633	1	3	theme	cell-wall	250:258	arg1	residues					238:245	diverse sugar residues	224:245	diverse sugar residues	224:245	BACKGROUND AND AIMS During evolution, plants have acquired and/or lost diverse sugar residues as cell-wall constituents.
26113633	1	3	theme	cell-wall	250:258	arg1	constituents					260:271	cell-wall constituents	250:271	cell-wall constituents	250:271	BACKGROUND AND AIMS During evolution, plants have acquired and/or lost diverse sugar residues as cell-wall constituents.
26113633	0	4	theme	closest	68:74	arg1	composition					6:16	Sugar composition	0:16	Sugar composition of the pectic polysaccharides of charophytes	0:61	Sugar composition of the pectic polysaccharides of charophytes, the closest algal relatives of land-plants: presence of 3-O-methyl-D-galactose residues.
26113633	0	4	theme	closest	68:74	arg1	relatives					82:90	the closest algal relatives	64:90	the closest algal relatives of land-plants	64:105	Sugar composition of the pectic polysaccharides of charophytes, the closest algal relatives of land-plants: presence of 3-O-methyl-D-galactose residues.
26113633	5	5	theme	resonance	1068:1076	arg1	spectroscopy					1078:1089	(1)H/(13)C-nuclear magnetic resonance spectroscopy	1040:1089	(1)H/(13)C-nuclear magnetic resonance spectroscopy	1040:1089	One unusual monosaccharide, 'U', was characterized by (1)H/(13)C-nuclear magnetic resonance spectroscopy and also enzymically.
26113633	10	6	theme	Anthoceros	1666:1675	arg1	hemicelluloses					1677:1690	Anthoceros hemicelluloses	1666:1690	Anthoceros hemicelluloses	1666:1690	Anthoceros hemicelluloses were rich in glucose, xylose, galactose and arabinose (suggesting xyloglucan and arabinoxylan), none of which was consistently present in charophyte hemicelluloses.
26113633	10	7	from	arabinose	1736:1744	arg1	rich					1697:1700	rich	1697:1700	rich	1697:1700	Anthoceros hemicelluloses were rich in glucose, xylose, galactose and arabinose (suggesting xyloglucan and arabinoxylan), none of which was consistently present in charophyte hemicelluloses.
26113633	14	8	attach	presence	2185:2192	arg1	bryophytes					2264:2273	the 'intervening' bryophytes	2246:2273	the 'intervening' bryophytes	2246:2273	The presence of 3-MeGal in charophytes and lycophytes but not in the 'intervening' bryophytes confirms that cell-wall chemistry changed drastically between major phylogenetic grades.
26113633	14	8	attach	presence	2185:2192	arg1	charophytes					2208:2218	charophytes	2208:2218	charophytes	2208:2218	The presence of 3-MeGal in charophytes and lycophytes but not in the 'intervening' bryophytes confirms that cell-wall chemistry changed drastically between major phylogenetic grades.
26113633	14	8	attach	presence	2185:2192	arg2	3-MeGal					2197:2203	3-MeGal	2197:2203	3-MeGal	2197:2203	The presence of 3-MeGal in charophytes and lycophytes but not in the 'intervening' bryophytes confirms that cell-wall chemistry changed drastically between major phylogenetic grades.
26113633	9	9	theme	higher	1571:1576	arg1	charophytes					1579:1589	'higher' charophytes	1570:1589	'higher' charophytes	1570:1589	Pectins of 'higher' charophytes, especially Chara, contained little arabinose, instead possessing 3-MeGal.
26113633	13	10	theme	charophyte	2066:2075	arg1	hemicelluloses					2092:2105	charophyte and land-plant hemicelluloses	2066:2105	charophyte and land-plant hemicelluloses	2066:2105	In contrast, charophyte and land-plant hemicelluloses differ substantially, indicating major changes during terrestrialization.
26113633	6	11	theme	KEY	1113:1115	arg1	RESULTS					1117:1123	KEY RESULTS	1113:1123	KEY RESULTS 'U'	1113:1127	KEY RESULTS 'U' was identified as 3-O-methyl-D-galactose (3-MeGal).
26113633	6	11	theme	KEY	1113:1115	arg1	3-O-methyl-D-galactose					1147:1168	3-O-methyl-D-galactose	1147:1168	3-O-methyl-D-galactose (3-MeGal)	1147:1178	KEY RESULTS 'U' was identified as 3-O-methyl-D-galactose (3-MeGal).
26113633	2	12	theme	primordial	301:310	arg1	features					322:329	primordial cell-wall features	301:329	primordial cell-wall features that existed, and in some cases abruptly changed, during the momentous step whereby land-plants arose from charophytic algal ancestors	301:464	Of particular interest are primordial cell-wall features that existed, and in some cases abruptly changed, during the momentous step whereby land-plants arose from charophytic algal ancestors.
26113633	1	13	dep	BACKGROUND	153:162	arg1	acquired					203:210	acquired	203:210	acquired	203:210	BACKGROUND AND AIMS During evolution, plants have acquired and/or lost diverse sugar residues as cell-wall constituents.
26113633	1	13	dep	BACKGROUND	153:162	arg1	lost					219:222	lost	219:222	lost	219:222	BACKGROUND AND AIMS During evolution, plants have acquired and/or lost diverse sugar residues as cell-wall constituents.
26113633	10	14	from	present	1819:1825	arg1	hemicelluloses					1841:1854	charophyte hemicelluloses	1830:1854	charophyte hemicelluloses	1830:1854	Anthoceros hemicelluloses were rich in glucose, xylose, galactose and arabinose (suggesting xyloglucan and arabinoxylan), none of which was consistently present in charophyte hemicelluloses.
26113633	4	15	theme	6 m	864:866	arg1	°C					877:878	37 °C	874:878	37 °C	874:878	'Pectins' and 'hemicelluloses', operationally defined as extractable in oxalate (100 °C) and 6 m NaOH (37 °C), respectively, were acid- or Driselase-hydrolysed, and the monosaccharides analysed chromatographically.
26113633	4	15	theme	6 m	864:866	arg1	NaOH					868:871	6 m NaOH	864:871	6 m NaOH (37 °C)	864:879	'Pectins' and 'hemicelluloses', operationally defined as extractable in oxalate (100 °C) and 6 m NaOH (37 °C), respectively, were acid- or Driselase-hydrolysed, and the monosaccharides analysed chromatographically.
26113633	7	16	from	pectins	1185:1191	arg1	Klebsormidium					1204:1216	Klebsormidium	1204:1216	Klebsormidium	1204:1216	All pectins, except in Klebsormidium, contained acid- and Driselase-releasable galacturonate, suggesting homogalacturonan.
26113633	10	17	from	hemicelluloses	1841:1854	arg1	present					1819:1825	present	1819:1825	present	1819:1825	Anthoceros hemicelluloses were rich in glucose, xylose, galactose and arabinose (suggesting xyloglucan and arabinoxylan), none of which was consistently present in charophyte hemicelluloses.
26113633	3	18	theme	Anthoceros	749:758	arg1	land-plant					737:746	land-plant	737:746	land-plant	737:746	METHODS Polysaccharides were extracted from four charophyte orders [Chlorokybales (Chlorokybus atmophyticus), Klebsormidiales (Klebsormidium fluitans, K. subtile), Charales (Chara vulgaris, Nitella flexilis), Coleochaetales (Coleochaete scutata)] and an early-diverging land-plant (Anthoceros agrestis).
26113633	3	18	theme	Anthoceros	749:758	arg1	agrestis					760:767	Anthoceros agrestis	749:767	Anthoceros agrestis	749:767	METHODS Polysaccharides were extracted from four charophyte orders [Chlorokybales (Chlorokybus atmophyticus), Klebsormidiales (Klebsormidium fluitans, K. subtile), Charales (Chara vulgaris, Nitella flexilis), Coleochaetales (Coleochaete scutata)] and an early-diverging land-plant (Anthoceros agrestis).
26113633	7	19	theme	Driselase-releasable	1239:1258	arg1	galacturonate					1260:1272	acid- and Driselase-releasable galacturonate	1229:1272	acid- and Driselase-releasable galacturonate	1229:1272	All pectins, except in Klebsormidium, contained acid- and Driselase-releasable galacturonate, suggesting homogalacturonan.
26113633	2	20	theme	interest	288:295	arg1	features					322:329	primordial cell-wall features	301:329	primordial cell-wall features that existed, and in some cases abruptly changed, during the momentous step whereby land-plants arose from charophytic algal ancestors	301:464	Of particular interest are primordial cell-wall features that existed, and in some cases abruptly changed, during the momentous step whereby land-plants arose from charophytic algal ancestors.
26113633	10	21	located	present	1819:1825	arg2	none					1788:1791	none	1788:1791	none	1788:1791	Anthoceros hemicelluloses were rich in glucose, xylose, galactose and arabinose (suggesting xyloglucan and arabinoxylan), none of which was consistently present in charophyte hemicelluloses.
26113633	10	21	located	present	1819:1825	arg1	hemicelluloses					1841:1854	charophyte hemicelluloses	1830:1854	charophyte hemicelluloses	1830:1854	Anthoceros hemicelluloses were rich in glucose, xylose, galactose and arabinose (suggesting xyloglucan and arabinoxylan), none of which was consistently present in charophyte hemicelluloses.
26113633	8	22	from	only	1397:1400	arg1	higher					1406:1411	higher	1406:1411	higher	1406:1411	All pectins, without exception, released rhamnose and galactose on acid hydrolysis; however, only in 'higher' charophytes (Charales, Coleochaetales) and Anthoceros were these sugars also efficiently released by Driselase, suggesting rhamnogalacturonan-I.
26113633	0	23	theme	land-plants	95:105	arg1	composition					6:16	Sugar composition	0:16	Sugar composition of the pectic polysaccharides of charophytes	0:61	Sugar composition of the pectic polysaccharides of charophytes, the closest algal relatives of land-plants: presence of 3-O-methyl-D-galactose residues.
26113633	0	23	theme	land-plants	95:105	arg1	relatives					82:90	the closest algal relatives	64:90	the closest algal relatives of land-plants	64:105	Sugar composition of the pectic polysaccharides of charophytes, the closest algal relatives of land-plants: presence of 3-O-methyl-D-galactose residues.
26113633	8	24	from	higher	1406:1411	arg1	only					1397:1400	only	1397:1400	only	1397:1400	All pectins, without exception, released rhamnose and galactose on acid hydrolysis; however, only in 'higher' charophytes (Charales, Coleochaetales) and Anthoceros were these sugars also efficiently released by Driselase, suggesting rhamnogalacturonan-I.
26113633	2	25	theme	particular	277:286	arg1	interest					288:295	particular interest	277:295	particular interest	277:295	Of particular interest are primordial cell-wall features that existed, and in some cases abruptly changed, during the momentous step whereby land-plants arose from charophytic algal ancestors.
26113633	14	26	theme	cell-wall	2289:2297	arg1	chemistry					2299:2307	cell-wall chemistry	2289:2307	cell-wall chemistry	2289:2307	The presence of 3-MeGal in charophytes and lycophytes but not in the 'intervening' bryophytes confirms that cell-wall chemistry changed drastically between major phylogenetic grades.
26113633	2	27	theme	momentous	392:400	arg1	step					402:405	the momentous step	388:405	the momentous step whereby land-plants arose from charophytic algal ancestors	388:464	Of particular interest are primordial cell-wall features that existed, and in some cases abruptly changed, during the momentous step whereby land-plants arose from charophytic algal ancestors.
26113633	3	28	dep	[Chlorokybales	534:547	arg1	atmophyticus					562:573	Chlorokybus atmophyticus	550:573	Chlorokybus atmophyticus	550:573	METHODS Polysaccharides were extracted from four charophyte orders [Chlorokybales (Chlorokybus atmophyticus), Klebsormidiales (Klebsormidium fluitans, K. subtile), Charales (Chara vulgaris, Nitella flexilis), Coleochaetales (Coleochaete scutata)] and an early-diverging land-plant (Anthoceros agrestis).
26113633	0	29	theme	Sugar	0:4	arg1	composition					6:16	Sugar composition	0:16	Sugar composition of the pectic polysaccharides of charophytes	0:61	Sugar composition of the pectic polysaccharides of charophytes, the closest algal relatives of land-plants: presence of 3-O-methyl-D-galactose residues.
26113633	0	29	theme	Sugar	0:4	arg1	relatives					82:90	the closest algal relatives	64:90	the closest algal relatives of land-plants	64:105	Sugar composition of the pectic polysaccharides of charophytes, the closest algal relatives of land-plants: presence of 3-O-methyl-D-galactose residues.
26113633	12	30	contain	possessed	2021:2029	arg1	embryophytes					2000:2011	the first embryophytes	1990:2011	the first embryophytes	1990:2011	When conquering the land, the first embryophytes already possessed rhamnogalacturonan-I.
26113633	12	30	contain	possessed	2021:2029	arg2	rhamnogalacturonan-I					2031:2050	rhamnogalacturonan-I	2031:2050	rhamnogalacturonan-I	2031:2050	When conquering the land, the first embryophytes already possessed rhamnogalacturonan-I.
26113633	10	31	from	rich	1697:1700	arg1	galactose					1722:1730	galactose	1722:1730	galactose	1722:1730	Anthoceros hemicelluloses were rich in glucose, xylose, galactose and arabinose (suggesting xyloglucan and arabinoxylan), none of which was consistently present in charophyte hemicelluloses.
26113633	10	31	from	rich	1697:1700	arg1	xylose					1714:1719	xylose	1714:1719	xylose	1714:1719	Anthoceros hemicelluloses were rich in glucose, xylose, galactose and arabinose (suggesting xyloglucan and arabinoxylan), none of which was consistently present in charophyte hemicelluloses.
26113633	10	31	from	rich	1697:1700	arg1	glucose					1705:1711	glucose	1705:1711	glucose	1705:1711	Anthoceros hemicelluloses were rich in glucose, xylose, galactose and arabinose (suggesting xyloglucan and arabinoxylan), none of which was consistently present in charophyte hemicelluloses.
26113633	10	31	from	rich	1697:1700	arg1	arabinose					1736:1744	arabinose	1736:1744	arabinose	1736:1744	Anthoceros hemicelluloses were rich in glucose, xylose, galactose and arabinose (suggesting xyloglucan and arabinoxylan), none of which was consistently present in charophyte hemicelluloses.
26113633	13	32	theme	land-plant	2081:2090	arg1	hemicelluloses					2092:2105	charophyte and land-plant hemicelluloses	2066:2105	charophyte and land-plant hemicelluloses	2066:2105	In contrast, charophyte and land-plant hemicelluloses differ substantially, indicating major changes during terrestrialization.
26113633	3	33	dep	Charales	631:638	arg1	Chara					641:645	Chara	641:645	Chara	641:645	METHODS Polysaccharides were extracted from four charophyte orders [Chlorokybales (Chlorokybus atmophyticus), Klebsormidiales (Klebsormidium fluitans, K. subtile), Charales (Chara vulgaris, Nitella flexilis), Coleochaetales (Coleochaete scutata)] and an early-diverging land-plant (Anthoceros agrestis).
26113633	3	33	dep	Charales	631:638	arg1	flexilis					665:672	flexilis	665:672	flexilis	665:672	METHODS Polysaccharides were extracted from four charophyte orders [Chlorokybales (Chlorokybus atmophyticus), Klebsormidiales (Klebsormidium fluitans, K. subtile), Charales (Chara vulgaris, Nitella flexilis), Coleochaetales (Coleochaete scutata)] and an early-diverging land-plant (Anthoceros agrestis).
26113633	2	34	theme	algal	450:454	arg1	ancestors					456:464	charophytic algal ancestors	438:464	charophytic algal ancestors	438:464	Of particular interest are primordial cell-wall features that existed, and in some cases abruptly changed, during the momentous step whereby land-plants arose from charophytic algal ancestors.
26113633	10	35	from	xylose	1714:1719	arg1	rich					1697:1700	rich	1697:1700	rich	1697:1700	Anthoceros hemicelluloses were rich in glucose, xylose, galactose and arabinose (suggesting xyloglucan and arabinoxylan), none of which was consistently present in charophyte hemicelluloses.
26113633	6	36	dep	RESULTS	1117:1123	arg1	U					1126:1126	U	1126:1126	U	1126:1126	KEY RESULTS 'U' was identified as 3-O-methyl-D-galactose (3-MeGal).
26113633	2	37	theme	charophytic	438:448	arg1	ancestors					456:464	charophytic algal ancestors	438:464	charophytic algal ancestors	438:464	Of particular interest are primordial cell-wall features that existed, and in some cases abruptly changed, during the momentous step whereby land-plants arose from charophytic algal ancestors.
26113633	8	38	theme	only	1397:1400	arg1	Coleochaetales					1437:1450	Coleochaetales	1437:1450	Coleochaetales	1437:1450	All pectins, without exception, released rhamnose and galactose on acid hydrolysis; however, only in 'higher' charophytes (Charales, Coleochaetales) and Anthoceros were these sugars also efficiently released by Driselase, suggesting rhamnogalacturonan-I.
26113633	8	38	theme	only	1397:1400	arg1	charophytes					1414:1424	only in 'higher' charophytes	1397:1424	only in 'higher' charophytes (Charales, Coleochaetales)	1397:1451	All pectins, without exception, released rhamnose and galactose on acid hydrolysis; however, only in 'higher' charophytes (Charales, Coleochaetales) and Anthoceros were these sugars also efficiently released by Driselase, suggesting rhamnogalacturonan-I.
26113633	2	39	theme	cell-wall	312:320	arg1	features					322:329	primordial cell-wall features	301:329	primordial cell-wall features that existed, and in some cases abruptly changed, during the momentous step whereby land-plants arose from charophytic algal ancestors	301:464	Of particular interest are primordial cell-wall features that existed, and in some cases abruptly changed, during the momentous step whereby land-plants arose from charophytic algal ancestors.
26113633	3	40	dep	Chara	641:645	arg1	vulgaris					647:654	Chara vulgaris	641:654	Chara vulgaris	641:654	METHODS Polysaccharides were extracted from four charophyte orders [Chlorokybales (Chlorokybus atmophyticus), Klebsormidiales (Klebsormidium fluitans, K. subtile), Charales (Chara vulgaris, Nitella flexilis), Coleochaetales (Coleochaete scutata)] and an early-diverging land-plant (Anthoceros agrestis).
26113633	0	41	theme	polysaccharides	32:46	arg1	composition					6:16	Sugar composition	0:16	Sugar composition of the pectic polysaccharides of charophytes	0:61	Sugar composition of the pectic polysaccharides of charophytes, the closest algal relatives of land-plants: presence of 3-O-methyl-D-galactose residues.
26113633	0	41	theme	polysaccharides	32:46	arg1	relatives					82:90	the closest algal relatives	64:90	the closest algal relatives of land-plants	64:105	Sugar composition of the pectic polysaccharides of charophytes, the closest algal relatives of land-plants: presence of 3-O-methyl-D-galactose residues.
26113633	14	42	theme	phylogenetic	2343:2354	arg1	grades					2356:2361	major phylogenetic grades	2337:2361	major phylogenetic grades	2337:2361	The presence of 3-MeGal in charophytes and lycophytes but not in the 'intervening' bryophytes confirms that cell-wall chemistry changed drastically between major phylogenetic grades.
26113633	0	43	theme	residues	143:150	arg1	presence					108:115	presence	108:115	presence of 3-O-methyl-D-galactose residues	108:150	Sugar composition of the pectic polysaccharides of charophytes, the closest algal relatives of land-plants: presence of 3-O-methyl-D-galactose residues.
26113633	7	44	contain	contained	1219:1227	arg1	pectins					1185:1191	All pectins	1181:1191	All pectins	1181:1191	All pectins, except in Klebsormidium, contained acid- and Driselase-releasable galacturonate, suggesting homogalacturonan.
26113633	7	44	contain	contained	1219:1227	arg2	galacturonate					1260:1272	acid- and Driselase-releasable galacturonate	1229:1272	acid- and Driselase-releasable galacturonate	1229:1272	All pectins, except in Klebsormidium, contained acid- and Driselase-releasable galacturonate, suggesting homogalacturonan.
26113633	11	45	theme	CONCLUSIONS	1857:1867	arg1	Homogalacturonan					1869:1884	CONCLUSIONS Homogalacturonan	1857:1884	CONCLUSIONS Homogalacturonan	1857:1884	CONCLUSIONS Homogalacturonan is an ancient streptophyte feature, albeit secondarily lost in Klebsormidium.
26113633	11	45	theme	CONCLUSIONS	1857:1867	arg1	feature					1913:1919	an ancient streptophyte feature	1889:1919	an ancient streptophyte feature	1889:1919	CONCLUSIONS Homogalacturonan is an ancient streptophyte feature, albeit secondarily lost in Klebsormidium.
26113633	3	46	theme	METHODS	467:473	arg1	Polysaccharides					475:489	METHODS Polysaccharides	467:489	METHODS Polysaccharides	467:489	METHODS Polysaccharides were extracted from four charophyte orders [Chlorokybales (Chlorokybus atmophyticus), Klebsormidiales (Klebsormidium fluitans, K. subtile), Charales (Chara vulgaris, Nitella flexilis), Coleochaetales (Coleochaete scutata)] and an early-diverging land-plant (Anthoceros agrestis).
26113633	0	47	theme	pectic	25:30	arg1	polysaccharides					32:46	the pectic polysaccharides	21:46	the pectic polysaccharides of charophytes	21:61	Sugar composition of the pectic polysaccharides of charophytes, the closest algal relatives of land-plants: presence of 3-O-methyl-D-galactose residues.
26113633	0	48	theme	3-O-methyl-D-galactose	120:141	arg1	residues					143:150	3-O-methyl-D-galactose residues	120:150	3-O-methyl-D-galactose residues	120:150	Sugar composition of the pectic polysaccharides of charophytes, the closest algal relatives of land-plants: presence of 3-O-methyl-D-galactose residues.
26113633	13	49	theme	major	2140:2144	arg1	changes					2146:2152	major changes	2140:2152	major changes	2140:2152	In contrast, charophyte and land-plant hemicelluloses differ substantially, indicating major changes during terrestrialization.
26113633	7	50	theme	acid-	1229:1233	arg1	galacturonate					1260:1272	acid- and Driselase-releasable galacturonate	1229:1272	acid- and Driselase-releasable galacturonate	1229:1272	All pectins, except in Klebsormidium, contained acid- and Driselase-releasable galacturonate, suggesting homogalacturonan.
26113633	0	51	theme	charophytes	51:61	arg1	polysaccharides					32:46	the pectic polysaccharides	21:46	the pectic polysaccharides of charophytes	21:61	Sugar composition of the pectic polysaccharides of charophytes, the closest algal relatives of land-plants: presence of 3-O-methyl-D-galactose residues.
26113633	14	52	theme	3-MeGal	2197:2203	arg1	presence					2185:2192	The presence	2181:2192	The presence of 3-MeGal in charophytes and lycophytes but not in the 'intervening' bryophytes	2181:2273	The presence of 3-MeGal in charophytes and lycophytes but not in the 'intervening' bryophytes confirms that cell-wall chemistry changed drastically between major phylogenetic grades.
26113633	14	53	from	presence	2185:2192	arg1	bryophytes					2264:2273	the 'intervening' bryophytes	2246:2273	the 'intervening' bryophytes	2246:2273	The presence of 3-MeGal in charophytes and lycophytes but not in the 'intervening' bryophytes confirms that cell-wall chemistry changed drastically between major phylogenetic grades.
26113633	14	53	from	presence	2185:2192	arg1	charophytes					2208:2218	charophytes	2208:2218	charophytes	2208:2218	The presence of 3-MeGal in charophytes and lycophytes but not in the 'intervening' bryophytes confirms that cell-wall chemistry changed drastically between major phylogenetic grades.
26113633	4	54	from	extractable	828:838	arg1	°C					877:878	37 °C	874:878	37 °C	874:878	'Pectins' and 'hemicelluloses', operationally defined as extractable in oxalate (100 °C) and 6 m NaOH (37 °C), respectively, were acid- or Driselase-hydrolysed, and the monosaccharides analysed chromatographically.
26113633	4	54	from	extractable	828:838	arg1	oxalate					843:849	oxalate	843:849	oxalate (100 °C)	843:858	'Pectins' and 'hemicelluloses', operationally defined as extractable in oxalate (100 °C) and 6 m NaOH (37 °C), respectively, were acid- or Driselase-hydrolysed, and the monosaccharides analysed chromatographically.
26113633	4	54	from	extractable	828:838	arg1	°C					856:857	100 °C	852:857	100 °C	852:857	'Pectins' and 'hemicelluloses', operationally defined as extractable in oxalate (100 °C) and 6 m NaOH (37 °C), respectively, were acid- or Driselase-hydrolysed, and the monosaccharides analysed chromatographically.
26113633	4	54	from	extractable	828:838	arg1	NaOH					868:871	6 m NaOH	864:871	6 m NaOH (37 °C)	864:879	'Pectins' and 'hemicelluloses', operationally defined as extractable in oxalate (100 °C) and 6 m NaOH (37 °C), respectively, were acid- or Driselase-hydrolysed, and the monosaccharides analysed chromatographically.
26113633	3	55	theme	charophyte	516:525	arg1	Klebsormidiales					577:591	Klebsormidiales	577:591	Klebsormidiales	577:591	METHODS Polysaccharides were extracted from four charophyte orders [Chlorokybales (Chlorokybus atmophyticus), Klebsormidiales (Klebsormidium fluitans, K. subtile), Charales (Chara vulgaris, Nitella flexilis), Coleochaetales (Coleochaete scutata)] and an early-diverging land-plant (Anthoceros agrestis).
26113633	3	55	theme	charophyte	516:525	arg1	orders					527:532	four charophyte orders [Chlorokybales (Chlorokybus atmophyticus), Klebsormidiales (Klebsormidium fluitans, K. subtile), Charales (Chara vulgaris, Nitella flexilis), Coleochaetales (Coleochaete scutata)] and an early-diverging land-plant (Anthoceros agrestis)	511:768	four charophyte orders [Chlorokybales (Chlorokybus atmophyticus), Klebsormidiales (Klebsormidium fluitans, K. subtile), Charales (Chara vulgaris, Nitella flexilis), Coleochaetales (Coleochaete scutata)] and an early-diverging land-plant (Anthoceros agrestis)	511:768	METHODS Polysaccharides were extracted from four charophyte orders [Chlorokybales (Chlorokybus atmophyticus), Klebsormidiales (Klebsormidium fluitans, K. subtile), Charales (Chara vulgaris, Nitella flexilis), Coleochaetales (Coleochaete scutata)] and an early-diverging land-plant (Anthoceros agrestis).
26113633	3	55	theme	charophyte	516:525	arg1	[Chlorokybales					534:547	[Chlorokybales	534:547	[Chlorokybales (Chlorokybus atmophyticus)	534:574	METHODS Polysaccharides were extracted from four charophyte orders [Chlorokybales (Chlorokybus atmophyticus), Klebsormidiales (Klebsormidium fluitans, K. subtile), Charales (Chara vulgaris, Nitella flexilis), Coleochaetales (Coleochaete scutata)] and an early-diverging land-plant (Anthoceros agrestis).
26113633	11	56	theme	ancient	1892:1898	arg1	Homogalacturonan					1869:1884	CONCLUSIONS Homogalacturonan	1857:1884	CONCLUSIONS Homogalacturonan	1857:1884	CONCLUSIONS Homogalacturonan is an ancient streptophyte feature, albeit secondarily lost in Klebsormidium.
26113633	11	56	theme	ancient	1892:1898	arg1	feature					1913:1919	an ancient streptophyte feature	1889:1919	an ancient streptophyte feature	1889:1919	CONCLUSIONS Homogalacturonan is an ancient streptophyte feature, albeit secondarily lost in Klebsormidium.
26113633	8	57	dep	released	1503:1510	arg1	sugars					1479:1484	these sugars	1473:1484	these sugars	1473:1484	All pectins, without exception, released rhamnose and galactose on acid hydrolysis; however, only in 'higher' charophytes (Charales, Coleochaetales) and Anthoceros were these sugars also efficiently released by Driselase, suggesting rhamnogalacturonan-I.
26113633	9	58	theme	little	1620:1625	arg1	arabinose					1627:1635	little arabinose	1620:1635	little arabinose	1620:1635	Pectins of 'higher' charophytes, especially Chara, contained little arabinose, instead possessing 3-MeGal.
26113633	10	59	from	glucose	1705:1711	arg1	rich					1697:1700	rich	1697:1700	rich	1697:1700	Anthoceros hemicelluloses were rich in glucose, xylose, galactose and arabinose (suggesting xyloglucan and arabinoxylan), none of which was consistently present in charophyte hemicelluloses.
26113633	9	60	theme	charophytes	1579:1589	arg1	Pectins					1559:1565	Pectins	1559:1565	Pectins of 'higher' charophytes	1559:1589	Pectins of 'higher' charophytes, especially Chara, contained little arabinose, instead possessing 3-MeGal.
26113633	9	60	theme	charophytes	1579:1589	arg1	Chara					1603:1607	Chara	1603:1607	Chara	1603:1607	Pectins of 'higher' charophytes, especially Chara, contained little arabinose, instead possessing 3-MeGal.
26113633	10	61	theme	charophyte	1830:1839	arg1	hemicelluloses					1841:1854	charophyte hemicelluloses	1830:1854	charophyte hemicelluloses	1830:1854	Anthoceros hemicelluloses were rich in glucose, xylose, galactose and arabinose (suggesting xyloglucan and arabinoxylan), none of which was consistently present in charophyte hemicelluloses.
26113633	14	62	theme	intervening	2251:2261	arg1	bryophytes					2264:2273	the 'intervening' bryophytes	2246:2273	the 'intervening' bryophytes	2246:2273	The presence of 3-MeGal in charophytes and lycophytes but not in the 'intervening' bryophytes confirms that cell-wall chemistry changed drastically between major phylogenetic grades.
26113633	3	63	dep	Klebsormidiales	577:591	arg1	Charales					631:638	Charales	631:638	Charales	631:638	METHODS Polysaccharides were extracted from four charophyte orders [Chlorokybales (Chlorokybus atmophyticus), Klebsormidiales (Klebsormidium fluitans, K. subtile), Charales (Chara vulgaris, Nitella flexilis), Coleochaetales (Coleochaete scutata)] and an early-diverging land-plant (Anthoceros agrestis).
26113633	3	63	dep	Klebsormidiales	577:591	arg1	Coleochaetales					676:689	Coleochaetales	676:689	Coleochaetales	676:689	METHODS Polysaccharides were extracted from four charophyte orders [Chlorokybales (Chlorokybus atmophyticus), Klebsormidiales (Klebsormidium fluitans, K. subtile), Charales (Chara vulgaris, Nitella flexilis), Coleochaetales (Coleochaete scutata)] and an early-diverging land-plant (Anthoceros agrestis).
26113633	1	64	theme	diverse	224:230	arg1	residues					238:245	diverse sugar residues	224:245	diverse sugar residues	224:245	BACKGROUND AND AIMS During evolution, plants have acquired and/or lost diverse sugar residues as cell-wall constituents.
26113633	1	64	theme	diverse	224:230	arg1	constituents					260:271	cell-wall constituents	250:271	cell-wall constituents	250:271	BACKGROUND AND AIMS During evolution, plants have acquired and/or lost diverse sugar residues as cell-wall constituents.
26113633	3	65	dep	orders	527:532	arg1	land-plant					737:746	land-plant	737:746	land-plant	737:746	METHODS Polysaccharides were extracted from four charophyte orders [Chlorokybales (Chlorokybus atmophyticus), Klebsormidiales (Klebsormidium fluitans, K. subtile), Charales (Chara vulgaris, Nitella flexilis), Coleochaetales (Coleochaete scutata)] and an early-diverging land-plant (Anthoceros agrestis).
26113633	3	65	dep	orders	527:532	arg1	agrestis					760:767	Anthoceros agrestis	749:767	Anthoceros agrestis	749:767	METHODS Polysaccharides were extracted from four charophyte orders [Chlorokybales (Chlorokybus atmophyticus), Klebsormidiales (Klebsormidium fluitans, K. subtile), Charales (Chara vulgaris, Nitella flexilis), Coleochaetales (Coleochaete scutata)] and an early-diverging land-plant (Anthoceros agrestis).
26113633	3	65	dep	orders	527:532	arg1	orders					527:532	four charophyte orders [Chlorokybales (Chlorokybus atmophyticus), Klebsormidiales (Klebsormidium fluitans, K. subtile), Charales (Chara vulgaris, Nitella flexilis), Coleochaetales (Coleochaete scutata)] and an early-diverging land-plant (Anthoceros agrestis)	511:768	four charophyte orders [Chlorokybales (Chlorokybus atmophyticus), Klebsormidiales (Klebsormidium fluitans, K. subtile), Charales (Chara vulgaris, Nitella flexilis), Coleochaetales (Coleochaete scutata)] and an early-diverging land-plant (Anthoceros agrestis)	511:768	METHODS Polysaccharides were extracted from four charophyte orders [Chlorokybales (Chlorokybus atmophyticus), Klebsormidiales (Klebsormidium fluitans, K. subtile), Charales (Chara vulgaris, Nitella flexilis), Coleochaetales (Coleochaete scutata)] and an early-diverging land-plant (Anthoceros agrestis).
26113633	3	65	dep	orders	527:532	arg1	Klebsormidiales					577:591	Klebsormidiales	577:591	Klebsormidiales	577:591	METHODS Polysaccharides were extracted from four charophyte orders [Chlorokybales (Chlorokybus atmophyticus), Klebsormidiales (Klebsormidium fluitans, K. subtile), Charales (Chara vulgaris, Nitella flexilis), Coleochaetales (Coleochaete scutata)] and an early-diverging land-plant (Anthoceros agrestis).
26113633	3	65	dep	orders	527:532	arg1	subtile					621:627	subtile	621:627	subtile	621:627	METHODS Polysaccharides were extracted from four charophyte orders [Chlorokybales (Chlorokybus atmophyticus), Klebsormidiales (Klebsormidium fluitans, K. subtile), Charales (Chara vulgaris, Nitella flexilis), Coleochaetales (Coleochaete scutata)] and an early-diverging land-plant (Anthoceros agrestis).
26113633	3	65	dep	orders	527:532	arg1	[Chlorokybales					534:547	[Chlorokybales	534:547	[Chlorokybales (Chlorokybus atmophyticus)	534:574	METHODS Polysaccharides were extracted from four charophyte orders [Chlorokybales (Chlorokybus atmophyticus), Klebsormidiales (Klebsormidium fluitans, K. subtile), Charales (Chara vulgaris, Nitella flexilis), Coleochaetales (Coleochaete scutata)] and an early-diverging land-plant (Anthoceros agrestis).
26113633	8	66	theme	acid	1371:1374	arg1	hydrolysis					1376:1385	acid hydrolysis	1371:1385	acid hydrolysis	1371:1385	All pectins, without exception, released rhamnose and galactose on acid hydrolysis; however, only in 'higher' charophytes (Charales, Coleochaetales) and Anthoceros were these sugars also efficiently released by Driselase, suggesting rhamnogalacturonan-I.
26113633	10	67	attach	present	1819:1825	arg2	none					1788:1791	none	1788:1791	none	1788:1791	Anthoceros hemicelluloses were rich in glucose, xylose, galactose and arabinose (suggesting xyloglucan and arabinoxylan), none of which was consistently present in charophyte hemicelluloses.
26113633	10	67	attach	present	1819:1825	arg1	hemicelluloses					1841:1854	charophyte hemicelluloses	1830:1854	charophyte hemicelluloses	1830:1854	Anthoceros hemicelluloses were rich in glucose, xylose, galactose and arabinose (suggesting xyloglucan and arabinoxylan), none of which was consistently present in charophyte hemicelluloses.
26113633	1	68	theme	sugar	232:236	arg1	residues					238:245	diverse sugar residues	224:245	diverse sugar residues	224:245	BACKGROUND AND AIMS During evolution, plants have acquired and/or lost diverse sugar residues as cell-wall constituents.
26113633	1	68	theme	sugar	232:236	arg1	constituents					260:271	cell-wall constituents	250:271	cell-wall constituents	250:271	BACKGROUND AND AIMS During evolution, plants have acquired and/or lost diverse sugar residues as cell-wall constituents.
26113633	12	69	theme	first	1994:1998	arg1	embryophytes					2000:2011	the first embryophytes	1990:2011	the first embryophytes	1990:2011	When conquering the land, the first embryophytes already possessed rhamnogalacturonan-I.
26113633	9	70	contain	contained	1610:1618	arg1	Pectins					1559:1565	Pectins	1559:1565	Pectins of 'higher' charophytes	1559:1589	Pectins of 'higher' charophytes, especially Chara, contained little arabinose, instead possessing 3-MeGal.
26113633	9	70	contain	contained	1610:1618	arg1	Chara					1603:1607	Chara	1603:1607	Chara	1603:1607	Pectins of 'higher' charophytes, especially Chara, contained little arabinose, instead possessing 3-MeGal.
26113633	9	70	contain	contained	1610:1618	arg2	arabinose					1627:1635	little arabinose	1620:1635	little arabinose	1620:1635	Pectins of 'higher' charophytes, especially Chara, contained little arabinose, instead possessing 3-MeGal.
26113633	0	71	dep	composition	6:16	arg1	presence					108:115	presence	108:115	presence of 3-O-methyl-D-galactose residues	108:150	Sugar composition of the pectic polysaccharides of charophytes, the closest algal relatives of land-plants: presence of 3-O-methyl-D-galactose residues.
26113633	11	72	theme	streptophyte	1900:1911	arg1	Homogalacturonan					1869:1884	CONCLUSIONS Homogalacturonan	1857:1884	CONCLUSIONS Homogalacturonan	1857:1884	CONCLUSIONS Homogalacturonan is an ancient streptophyte feature, albeit secondarily lost in Klebsormidium.
26113633	11	72	theme	streptophyte	1900:1911	arg1	feature					1913:1919	an ancient streptophyte feature	1889:1919	an ancient streptophyte feature	1889:1919	CONCLUSIONS Homogalacturonan is an ancient streptophyte feature, albeit secondarily lost in Klebsormidium.
26113633	10	73	dep	glucose	1705:1711	arg1	suggesting					1747:1756	suggesting	1747:1756	suggesting xyloglucan and arabinoxylan	1747:1784	Anthoceros hemicelluloses were rich in glucose, xylose, galactose and arabinose (suggesting xyloglucan and arabinoxylan), none of which was consistently present in charophyte hemicelluloses.
26113633	5	74	theme	C-nuclear	1049:1057	arg1	spectroscopy					1078:1089	(1)H/(13)C-nuclear magnetic resonance spectroscopy	1040:1089	(1)H/(13)C-nuclear magnetic resonance spectroscopy	1040:1089	One unusual monosaccharide, 'U', was characterized by (1)H/(13)C-nuclear magnetic resonance spectroscopy and also enzymically.
26113633	5	75	theme	unusual	990:996	arg1	monosaccharide					998:1011	One unusual monosaccharide	986:1011	One unusual monosaccharide	986:1011	One unusual monosaccharide, 'U', was characterized by (1)H/(13)C-nuclear magnetic resonance spectroscopy and also enzymically.
26113633	5	75	theme	unusual	990:996	arg1	U					1015:1015	'U'	1014:1016	'U'	1014:1016	One unusual monosaccharide, 'U', was characterized by (1)H/(13)C-nuclear magnetic resonance spectroscopy and also enzymically.
26113633	10	76	from	galactose	1722:1730	arg1	rich					1697:1700	rich	1697:1700	rich	1697:1700	Anthoceros hemicelluloses were rich in glucose, xylose, galactose and arabinose (suggesting xyloglucan and arabinoxylan), none of which was consistently present in charophyte hemicelluloses.
28281873	6	0	theme	low-abundant	1349:1360	arg1	glycoforms					1371:1380	low-abundant uncommon glycoforms	1349:1380	low-abundant uncommon glycoforms	1349:1380	The glycosylation characterization data derived from these approaches were found to be mutually consistent in composition profiling, and complementary in identification and relative-quantitation of low-abundant uncommon glycoforms.
28281873	6	1	from	complementary	1288:1300	arg1	identification					1305:1318	identification	1305:1318	identification	1305:1318	The glycosylation characterization data derived from these approaches were found to be mutually consistent in composition profiling, and complementary in identification and relative-quantitation of low-abundant uncommon glycoforms.
28281873	6	1	from	complementary	1288:1300	arg1	relative-quantitation					1324:1344	relative-quantitation	1324:1344	relative-quantitation	1324:1344	The glycosylation characterization data derived from these approaches were found to be mutually consistent in composition profiling, and complementary in identification and relative-quantitation of low-abundant uncommon glycoforms.
28281873	7	2	theme	light	1497:1501	arg1	chains					1503:1508	light chains	1497:1508	light chains	1497:1508	In addition, multiple other sources of micro-heterogeneity, such as glycation, lack of glycosylation, and loss of light chains, could be detected by this approach, and the contribution of multiple types of modifications to the overall micro-heterogeneity could be assessed using our superposition algorithm.
28281873	8	3	theme	mAbs	1786:1789	arg1	characterization					1766:1781	reliable and thorough characterization	1744:1781	reliable and thorough characterization of mAbs	1744:1789	Our data demonstrate that the hybrid strategy allows reliable and thorough characterization of mAbs, revealing product characteristics that would easily be missed if only a single approach were used.
28281873	1	4	theme	modifications	292:304	arg1	types					264:268	multiple types	255:268	multiple types of post-translational modifications (PTMs)	255:311	For therapeutic monoclonal antibodies (mAbs), detailed analysis of the structural integrity and heterogeneity, which results from multiple types of post-translational modifications (PTMs), is relevant to various processes, including product characterization, storage stability and quality control.
28281873	1	5	theme	therapeutic	129:139	arg1	antibodies					152:161	therapeutic monoclonal antibodies	129:161	therapeutic monoclonal antibodies (mAbs)	129:168	For therapeutic monoclonal antibodies (mAbs), detailed analysis of the structural integrity and heterogeneity, which results from multiple types of post-translational modifications (PTMs), is relevant to various processes, including product characterization, storage stability and quality control.
28281873	1	5	theme	therapeutic	129:139	arg1	mAbs					164:167	mAbs	164:167	mAbs	164:167	For therapeutic monoclonal antibodies (mAbs), detailed analysis of the structural integrity and heterogeneity, which results from multiple types of post-translational modifications (PTMs), is relevant to various processes, including product characterization, storage stability and quality control.
28281873	4	6	theme	glycan	897:902	arg1	approaches					846:855	2 state-of-the-art MS-based approaches	818:855	2 state-of-the-art MS-based approaches	818:855	Here, we tested a hybrid strategy for the comprehensive characterization of micro-heterogeneity by integrating 2 state-of-the-art MS-based approaches, high-resolution native MS and targeted glycan profiling, to perform complementary analysis at the intact protein level and released glycan level, respectively.
28281873	4	6	theme	glycan	897:902	arg1	profiling					904:912	targeted glycan profiling	888:912	targeted glycan profiling	888:912	Here, we tested a hybrid strategy for the comprehensive characterization of micro-heterogeneity by integrating 2 state-of-the-art MS-based approaches, high-resolution native MS and targeted glycan profiling, to perform complementary analysis at the intact protein level and released glycan level, respectively.
28281873	3	7	theme	indispensable	589:601	arg1	information					675:685	unique structural information	657:685	unique structural information at multiple levels	657:704	As a nearly indispensable tool in mAb analysis, mass spectrometry (MS) provides unique structural information at multiple levels.
28281873	3	7	theme	indispensable	589:601	arg1	tool					603:606	a nearly indispensable tool	580:606	a nearly indispensable tool in mAb analysis	580:622	As a nearly indispensable tool in mAb analysis, mass spectrometry (MS) provides unique structural information at multiple levels.
28281873	1	8	theme	structural	196:205	arg1	integrity					207:215	the structural integrity	192:215	the structural integrity	192:215	For therapeutic monoclonal antibodies (mAbs), detailed analysis of the structural integrity and heterogeneity, which results from multiple types of post-translational modifications (PTMs), is relevant to various processes, including product characterization, storage stability and quality control.
28281873	4	9	theme	protein	963:969	arg1	level					971:975	the intact protein level	952:975	the intact protein level	952:975	Here, we tested a hybrid strategy for the comprehensive characterization of micro-heterogeneity by integrating 2 state-of-the-art MS-based approaches, high-resolution native MS and targeted glycan profiling, to perform complementary analysis at the intact protein level and released glycan level, respectively.
28281873	5	10	theme	engineered	1080:1089	arg1	IgG4s					1101:1105	engineered half-body IgG4s	1080:1105	engineered half-body IgG4s	1080:1105	We compared the performance of these methods using samples of engineered half-body IgG4s and a panel of mAbs approved for human use.
28281873	1	11	theme	monoclonal	141:150	arg1	antibodies					152:161	therapeutic monoclonal antibodies	129:161	therapeutic monoclonal antibodies (mAbs)	129:168	For therapeutic monoclonal antibodies (mAbs), detailed analysis of the structural integrity and heterogeneity, which results from multiple types of post-translational modifications (PTMs), is relevant to various processes, including product characterization, storage stability and quality control.
28281873	1	11	theme	monoclonal	141:150	arg1	mAbs					164:167	mAbs	164:167	mAbs	164:167	For therapeutic monoclonal antibodies (mAbs), detailed analysis of the structural integrity and heterogeneity, which results from multiple types of post-translational modifications (PTMs), is relevant to various processes, including product characterization, storage stability and quality control.
28281873	7	12	theme	superposition	1666:1678	arg1	algorithm					1680:1688	our superposition algorithm	1662:1688	our superposition algorithm	1662:1688	In addition, multiple other sources of micro-heterogeneity, such as glycation, lack of glycosylation, and loss of light chains, could be detected by this approach, and the contribution of multiple types of modifications to the overall micro-heterogeneity could be assessed using our superposition algorithm.
28281873	4	13	theme	hybrid	725:730	arg1	strategy					732:739	a hybrid strategy	723:739	a hybrid strategy for the comprehensive characterization of micro-heterogeneity	723:801	Here, we tested a hybrid strategy for the comprehensive characterization of micro-heterogeneity by integrating 2 state-of-the-art MS-based approaches, high-resolution native MS and targeted glycan profiling, to perform complementary analysis at the intact protein level and released glycan level, respectively.
28281873	6	14	theme	glycoforms	1371:1380	arg1	identification					1305:1318	identification	1305:1318	identification	1305:1318	The glycosylation characterization data derived from these approaches were found to be mutually consistent in composition profiling, and complementary in identification and relative-quantitation of low-abundant uncommon glycoforms.
28281873	6	14	theme	glycoforms	1371:1380	arg1	relative-quantitation					1324:1344	relative-quantitation	1324:1344	relative-quantitation	1324:1344	The glycosylation characterization data derived from these approaches were found to be mutually consistent in composition profiling, and complementary in identification and relative-quantitation of low-abundant uncommon glycoforms.
28281873	5	15	theme	IgG4s	1101:1105	arg1	panel					1113:1117	a panel	1111:1117	a panel of mAbs approved for human use	1111:1148	We compared the performance of these methods using samples of engineered half-body IgG4s and a panel of mAbs approved for human use.
28281873	5	15	theme	IgG4s	1101:1105	arg1	samples					1069:1075	samples	1069:1075	samples of engineered half-body IgG4s	1069:1105	We compared the performance of these methods using samples of engineered half-body IgG4s and a panel of mAbs approved for human use.
28281873	0	16	theme	antibodies	73:82	arg1	micro-heterogeneity					14:32	micro-heterogeneity	14:32	micro-heterogeneity	14:32	Resolving the micro-heterogeneity and structural integrity of monoclonal antibodies by hybrid mass spectrometric approaches.
28281873	0	16	theme	antibodies	73:82	arg1	integrity					49:57	structural integrity	38:57	structural integrity	38:57	Resolving the micro-heterogeneity and structural integrity of monoclonal antibodies by hybrid mass spectrometric approaches.
28281873	3	17	theme	mass	625:628	arg1	MS					644:645	MS	644:645	MS	644:645	As a nearly indispensable tool in mAb analysis, mass spectrometry (MS) provides unique structural information at multiple levels.
28281873	3	17	theme	mass	625:628	arg1	spectrometry					630:641	mass spectrometry	625:641	mass spectrometry (MS)	625:646	As a nearly indispensable tool in mAb analysis, mass spectrometry (MS) provides unique structural information at multiple levels.
28281873	0	18	theme	hybrid	87:92	arg1	approaches					113:122	hybrid mass spectrometric approaches	87:122	hybrid mass spectrometric approaches	87:122	Resolving the micro-heterogeneity and structural integrity of monoclonal antibodies by hybrid mass spectrometric approaches.
28281873	1	19	theme	detailed	171:178	arg1	analysis					180:187	detailed analysis	171:187	detailed analysis	171:187	For therapeutic monoclonal antibodies (mAbs), detailed analysis of the structural integrity and heterogeneity, which results from multiple types of post-translational modifications (PTMs), is relevant to various processes, including product characterization, storage stability and quality control.
28281873	4	20	theme	glycan	990:995	arg1	level					997:1001	released glycan level	981:1001	released glycan level	981:1001	Here, we tested a hybrid strategy for the comprehensive characterization of micro-heterogeneity by integrating 2 state-of-the-art MS-based approaches, high-resolution native MS and targeted glycan profiling, to perform complementary analysis at the intact protein level and released glycan level, respectively.
28281873	6	21	from	consistent	1247:1256	arg1	profiling					1273:1281	composition profiling	1261:1281	composition profiling	1261:1281	The glycosylation characterization data derived from these approaches were found to be mutually consistent in composition profiling, and complementary in identification and relative-quantitation of low-abundant uncommon glycoforms.
28281873	0	22	theme	spectrometric	99:111	arg1	approaches					113:122	hybrid mass spectrometric approaches	87:122	hybrid mass spectrometric approaches	87:122	Resolving the micro-heterogeneity and structural integrity of monoclonal antibodies by hybrid mass spectrometric approaches.
28281873	5	23	theme	approved	1127:1134	arg1	mAbs					1122:1125	mAbs	1122:1125	mAbs approved for human use	1122:1148	We compared the performance of these methods using samples of engineered half-body IgG4s and a panel of mAbs approved for human use.
28281873	6	24	from	identification	1305:1318	arg1	complementary					1288:1300	complementary	1288:1300	complementary	1288:1300	The glycosylation characterization data derived from these approaches were found to be mutually consistent in composition profiling, and complementary in identification and relative-quantitation of low-abundant uncommon glycoforms.
28281873	1	25	theme	storage	384:390	arg1	stability					392:400	storage stability	384:400	storage stability	384:400	For therapeutic monoclonal antibodies (mAbs), detailed analysis of the structural integrity and heterogeneity, which results from multiple types of post-translational modifications (PTMs), is relevant to various processes, including product characterization, storage stability and quality control.
28281873	2	26	theme	bioanalytical	467:479	arg1	techniques					481:490	new bioanalytical techniques	463:490	new bioanalytical techniques	463:490	Despite the recent rapid development of new bioanalytical techniques, it is still challenging to completely characterize the proteoform profile of a mAb.
28281873	8	27	used	used	1885:1888	arg2	approach					1871:1878	only a single approach	1857:1878	only a single approach	1857:1878	Our data demonstrate that the hybrid strategy allows reliable and thorough characterization of mAbs, revealing product characteristics that would easily be missed if only a single approach were used.
28281873	7	28	theme	multiple	1396:1403	arg1	glycation					1451:1459	glycation	1451:1459	glycation	1451:1459	In addition, multiple other sources of micro-heterogeneity, such as glycation, lack of glycosylation, and loss of light chains, could be detected by this approach, and the contribution of multiple types of modifications to the overall micro-heterogeneity could be assessed using our superposition algorithm.
28281873	7	28	theme	multiple	1396:1403	arg1	lack					1462:1465	lack	1462:1465	lack of glycosylation	1462:1482	In addition, multiple other sources of micro-heterogeneity, such as glycation, lack of glycosylation, and loss of light chains, could be detected by this approach, and the contribution of multiple types of modifications to the overall micro-heterogeneity could be assessed using our superposition algorithm.
28281873	7	28	theme	multiple	1396:1403	arg1	loss					1489:1492	loss	1489:1492	loss of light chains	1489:1508	In addition, multiple other sources of micro-heterogeneity, such as glycation, lack of glycosylation, and loss of light chains, could be detected by this approach, and the contribution of multiple types of modifications to the overall micro-heterogeneity could be assessed using our superposition algorithm.
28281873	7	28	theme	multiple	1396:1403	arg1	sources					1411:1417	multiple other sources	1396:1417	multiple other sources	1396:1417	In addition, multiple other sources of micro-heterogeneity, such as glycation, lack of glycosylation, and loss of light chains, could be detected by this approach, and the contribution of multiple types of modifications to the overall micro-heterogeneity could be assessed using our superposition algorithm.
28281873	4	29	theme	comprehensive	749:761	arg1	characterization					763:778	the comprehensive characterization	745:778	the comprehensive characterization of micro-heterogeneity	745:801	Here, we tested a hybrid strategy for the comprehensive characterization of micro-heterogeneity by integrating 2 state-of-the-art MS-based approaches, high-resolution native MS and targeted glycan profiling, to perform complementary analysis at the intact protein level and released glycan level, respectively.
28281873	2	30	theme	rapid	442:446	arg1	development					448:458	the recent rapid development	431:458	the recent rapid development of new bioanalytical techniques	431:490	Despite the recent rapid development of new bioanalytical techniques, it is still challenging to completely characterize the proteoform profile of a mAb.
28281873	3	31	theme	unique	657:662	arg1	information					675:685	unique structural information	657:685	unique structural information at multiple levels	657:704	As a nearly indispensable tool in mAb analysis, mass spectrometry (MS) provides unique structural information at multiple levels.
28281873	3	31	theme	unique	657:662	arg1	tool					603:606	a nearly indispensable tool	580:606	a nearly indispensable tool in mAb analysis	580:622	As a nearly indispensable tool in mAb analysis, mass spectrometry (MS) provides unique structural information at multiple levels.
28281873	0	32	theme	structural	38:47	arg1	integrity					49:57	structural integrity	38:57	structural integrity	38:57	Resolving the micro-heterogeneity and structural integrity of monoclonal antibodies by hybrid mass spectrometric approaches.
28281873	7	33	theme	multiple	1571:1578	arg1	types					1580:1584	multiple types	1571:1584	multiple types of modifications	1571:1601	In addition, multiple other sources of micro-heterogeneity, such as glycation, lack of glycosylation, and loss of light chains, could be detected by this approach, and the contribution of multiple types of modifications to the overall micro-heterogeneity could be assessed using our superposition algorithm.
28281873	4	34	theme	native	874:879	arg1	MS					881:882	high-resolution native MS	858:882	high-resolution native MS	858:882	Here, we tested a hybrid strategy for the comprehensive characterization of micro-heterogeneity by integrating 2 state-of-the-art MS-based approaches, high-resolution native MS and targeted glycan profiling, to perform complementary analysis at the intact protein level and released glycan level, respectively.
28281873	4	34	theme	native	874:879	arg1	approaches					846:855	2 state-of-the-art MS-based approaches	818:855	2 state-of-the-art MS-based approaches	818:855	Here, we tested a hybrid strategy for the comprehensive characterization of micro-heterogeneity by integrating 2 state-of-the-art MS-based approaches, high-resolution native MS and targeted glycan profiling, to perform complementary analysis at the intact protein level and released glycan level, respectively.
28281873	6	35	theme	glycosylation	1155:1167	arg1	consistent					1247:1256	consistent	1247:1256	consistent	1247:1256	The glycosylation characterization data derived from these approaches were found to be mutually consistent in composition profiling, and complementary in identification and relative-quantitation of low-abundant uncommon glycoforms.
28281873	6	35	theme	glycosylation	1155:1167	arg1	data					1186:1189	The glycosylation characterization data	1151:1189	The glycosylation characterization data derived from these approaches	1151:1219	The glycosylation characterization data derived from these approaches were found to be mutually consistent in composition profiling, and complementary in identification and relative-quantitation of low-abundant uncommon glycoforms.
28281873	3	36	theme	multiple	690:697	arg1	levels					699:704	multiple levels	690:704	multiple levels	690:704	As a nearly indispensable tool in mAb analysis, mass spectrometry (MS) provides unique structural information at multiple levels.
28281873	0	37	dep	micro-heterogeneity	14:32	arg1	the					10:12	the	10:12	the	10:12	Resolving the micro-heterogeneity and structural integrity of monoclonal antibodies by hybrid mass spectrometric approaches.
28281873	4	38	theme	MS-based	837:844	arg1	MS					881:882	high-resolution native MS	858:882	high-resolution native MS	858:882	Here, we tested a hybrid strategy for the comprehensive characterization of micro-heterogeneity by integrating 2 state-of-the-art MS-based approaches, high-resolution native MS and targeted glycan profiling, to perform complementary analysis at the intact protein level and released glycan level, respectively.
28281873	4	38	theme	MS-based	837:844	arg1	profiling					904:912	targeted glycan profiling	888:912	targeted glycan profiling	888:912	Here, we tested a hybrid strategy for the comprehensive characterization of micro-heterogeneity by integrating 2 state-of-the-art MS-based approaches, high-resolution native MS and targeted glycan profiling, to perform complementary analysis at the intact protein level and released glycan level, respectively.
28281873	4	38	theme	MS-based	837:844	arg1	approaches					846:855	2 state-of-the-art MS-based approaches	818:855	2 state-of-the-art MS-based approaches	818:855	Here, we tested a hybrid strategy for the comprehensive characterization of micro-heterogeneity by integrating 2 state-of-the-art MS-based approaches, high-resolution native MS and targeted glycan profiling, to perform complementary analysis at the intact protein level and released glycan level, respectively.
28281873	6	39	attach	derived	1191:1197	arg2	data					1186:1189	The glycosylation characterization data	1151:1189	The glycosylation characterization data derived from these approaches	1151:1219	The glycosylation characterization data derived from these approaches were found to be mutually consistent in composition profiling, and complementary in identification and relative-quantitation of low-abundant uncommon glycoforms.
28281873	6	39	attach	derived	1191:1197	arg1	approaches					1210:1219	these approaches	1204:1219	these approaches	1204:1219	The glycosylation characterization data derived from these approaches were found to be mutually consistent in composition profiling, and complementary in identification and relative-quantitation of low-abundant uncommon glycoforms.
28281873	6	39	attach	derived	1191:1197	arg2	consistent					1247:1256	consistent	1247:1256	consistent	1247:1256	The glycosylation characterization data derived from these approaches were found to be mutually consistent in composition profiling, and complementary in identification and relative-quantitation of low-abundant uncommon glycoforms.
28281873	6	40	theme	uncommon	1362:1369	arg1	glycoforms					1371:1380	low-abundant uncommon glycoforms	1349:1380	low-abundant uncommon glycoforms	1349:1380	The glycosylation characterization data derived from these approaches were found to be mutually consistent in composition profiling, and complementary in identification and relative-quantitation of low-abundant uncommon glycoforms.
28281873	8	41	theme	reliable	1744:1751	arg1	characterization					1766:1781	reliable and thorough characterization	1744:1781	reliable and thorough characterization of mAbs	1744:1789	Our data demonstrate that the hybrid strategy allows reliable and thorough characterization of mAbs, revealing product characteristics that would easily be missed if only a single approach were used.
28281873	8	42	theme	thorough	1757:1764	arg1	characterization					1766:1781	reliable and thorough characterization	1744:1781	reliable and thorough characterization of mAbs	1744:1789	Our data demonstrate that the hybrid strategy allows reliable and thorough characterization of mAbs, revealing product characteristics that would easily be missed if only a single approach were used.
28281873	1	43	theme	post-translational	273:290	arg1	PTMs					307:310	PTMs	307:310	PTMs	307:310	For therapeutic monoclonal antibodies (mAbs), detailed analysis of the structural integrity and heterogeneity, which results from multiple types of post-translational modifications (PTMs), is relevant to various processes, including product characterization, storage stability and quality control.
28281873	1	43	theme	post-translational	273:290	arg1	modifications					292:304	post-translational modifications	273:304	post-translational modifications (PTMs)	273:311	For therapeutic monoclonal antibodies (mAbs), detailed analysis of the structural integrity and heterogeneity, which results from multiple types of post-translational modifications (PTMs), is relevant to various processes, including product characterization, storage stability and quality control.
28281873	7	44	theme	chains	1503:1508	arg1	glycation					1451:1459	glycation	1451:1459	glycation	1451:1459	In addition, multiple other sources of micro-heterogeneity, such as glycation, lack of glycosylation, and loss of light chains, could be detected by this approach, and the contribution of multiple types of modifications to the overall micro-heterogeneity could be assessed using our superposition algorithm.
28281873	7	44	theme	chains	1503:1508	arg1	loss					1489:1492	loss	1489:1492	loss of light chains	1489:1508	In addition, multiple other sources of micro-heterogeneity, such as glycation, lack of glycosylation, and loss of light chains, could be detected by this approach, and the contribution of multiple types of modifications to the overall micro-heterogeneity could be assessed using our superposition algorithm.
28281873	7	44	theme	chains	1503:1508	arg1	lack					1462:1465	lack	1462:1465	lack of glycosylation	1462:1482	In addition, multiple other sources of micro-heterogeneity, such as glycation, lack of glycosylation, and loss of light chains, could be detected by this approach, and the contribution of multiple types of modifications to the overall micro-heterogeneity could be assessed using our superposition algorithm.
28281873	4	45	theme	targeted	888:895	arg1	approaches					846:855	2 state-of-the-art MS-based approaches	818:855	2 state-of-the-art MS-based approaches	818:855	Here, we tested a hybrid strategy for the comprehensive characterization of micro-heterogeneity by integrating 2 state-of-the-art MS-based approaches, high-resolution native MS and targeted glycan profiling, to perform complementary analysis at the intact protein level and released glycan level, respectively.
28281873	4	45	theme	targeted	888:895	arg1	profiling					904:912	targeted glycan profiling	888:912	targeted glycan profiling	888:912	Here, we tested a hybrid strategy for the comprehensive characterization of micro-heterogeneity by integrating 2 state-of-the-art MS-based approaches, high-resolution native MS and targeted glycan profiling, to perform complementary analysis at the intact protein level and released glycan level, respectively.
28281873	8	46	theme	product	1802:1808	arg1	characteristics					1810:1824	product characteristics	1802:1824	product characteristics that would easily be missed if only a single approach were used	1802:1888	Our data demonstrate that the hybrid strategy allows reliable and thorough characterization of mAbs, revealing product characteristics that would easily be missed if only a single approach were used.
28281873	1	47	theme	integrity	207:215	arg1	analysis					180:187	detailed analysis	171:187	detailed analysis	171:187	For therapeutic monoclonal antibodies (mAbs), detailed analysis of the structural integrity and heterogeneity, which results from multiple types of post-translational modifications (PTMs), is relevant to various processes, including product characterization, storage stability and quality control.
28281873	4	48	theme	intact	956:961	arg1	level					971:975	the intact protein level	952:975	the intact protein level	952:975	Here, we tested a hybrid strategy for the comprehensive characterization of micro-heterogeneity by integrating 2 state-of-the-art MS-based approaches, high-resolution native MS and targeted glycan profiling, to perform complementary analysis at the intact protein level and released glycan level, respectively.
28281873	7	49	theme	glycosylation	1470:1482	arg1	glycation					1451:1459	glycation	1451:1459	glycation	1451:1459	In addition, multiple other sources of micro-heterogeneity, such as glycation, lack of glycosylation, and loss of light chains, could be detected by this approach, and the contribution of multiple types of modifications to the overall micro-heterogeneity could be assessed using our superposition algorithm.
28281873	7	49	theme	glycosylation	1470:1482	arg1	loss					1489:1492	loss	1489:1492	loss of light chains	1489:1508	In addition, multiple other sources of micro-heterogeneity, such as glycation, lack of glycosylation, and loss of light chains, could be detected by this approach, and the contribution of multiple types of modifications to the overall micro-heterogeneity could be assessed using our superposition algorithm.
28281873	7	49	theme	glycosylation	1470:1482	arg1	lack					1462:1465	lack	1462:1465	lack of glycosylation	1462:1482	In addition, multiple other sources of micro-heterogeneity, such as glycation, lack of glycosylation, and loss of light chains, could be detected by this approach, and the contribution of multiple types of modifications to the overall micro-heterogeneity could be assessed using our superposition algorithm.
28281873	3	50	from	levels	699:704	arg1	information					675:685	unique structural information	657:685	unique structural information at multiple levels	657:704	As a nearly indispensable tool in mAb analysis, mass spectrometry (MS) provides unique structural information at multiple levels.
28281873	3	50	from	levels	699:704	arg1	tool					603:606	a nearly indispensable tool	580:606	a nearly indispensable tool in mAb analysis	580:622	As a nearly indispensable tool in mAb analysis, mass spectrometry (MS) provides unique structural information at multiple levels.
28281873	1	51	theme	quality	406:412	arg1	control					414:420	quality control	406:420	quality control	406:420	For therapeutic monoclonal antibodies (mAbs), detailed analysis of the structural integrity and heterogeneity, which results from multiple types of post-translational modifications (PTMs), is relevant to various processes, including product characterization, storage stability and quality control.
28281873	3	52	theme	mAb	611:613	arg1	analysis					615:622	mAb analysis	611:622	mAb analysis	611:622	As a nearly indispensable tool in mAb analysis, mass spectrometry (MS) provides unique structural information at multiple levels.
28281873	1	53	theme	heterogeneity	221:233	arg1	analysis					180:187	detailed analysis	171:187	detailed analysis	171:187	For therapeutic monoclonal antibodies (mAbs), detailed analysis of the structural integrity and heterogeneity, which results from multiple types of post-translational modifications (PTMs), is relevant to various processes, including product characterization, storage stability and quality control.
28281873	5	54	theme	mAbs	1122:1125	arg1	panel					1113:1117	a panel	1111:1117	a panel of mAbs approved for human use	1111:1148	We compared the performance of these methods using samples of engineered half-body IgG4s and a panel of mAbs approved for human use.
28281873	5	54	theme	mAbs	1122:1125	arg1	samples					1069:1075	samples	1069:1075	samples of engineered half-body IgG4s	1069:1105	We compared the performance of these methods using samples of engineered half-body IgG4s and a panel of mAbs approved for human use.
28281873	7	55	located	detected	1520:1527	arg2	sources					1411:1417	multiple other sources	1396:1417	multiple other sources	1396:1417	In addition, multiple other sources of micro-heterogeneity, such as glycation, lack of glycosylation, and loss of light chains, could be detected by this approach, and the contribution of multiple types of modifications to the overall micro-heterogeneity could be assessed using our superposition algorithm.
28281873	7	55	located	detected	1520:1527	arg2	lack					1462:1465	lack	1462:1465	lack of glycosylation	1462:1482	In addition, multiple other sources of micro-heterogeneity, such as glycation, lack of glycosylation, and loss of light chains, could be detected by this approach, and the contribution of multiple types of modifications to the overall micro-heterogeneity could be assessed using our superposition algorithm.
28281873	7	55	located	detected	1520:1527	arg2	glycation					1451:1459	glycation	1451:1459	glycation	1451:1459	In addition, multiple other sources of micro-heterogeneity, such as glycation, lack of glycosylation, and loss of light chains, could be detected by this approach, and the contribution of multiple types of modifications to the overall micro-heterogeneity could be assessed using our superposition algorithm.
28281873	7	55	located	detected	1520:1527	arg1	addition					1386:1393	addition	1386:1393	addition	1386:1393	In addition, multiple other sources of micro-heterogeneity, such as glycation, lack of glycosylation, and loss of light chains, could be detected by this approach, and the contribution of multiple types of modifications to the overall micro-heterogeneity could be assessed using our superposition algorithm.
28281873	7	55	located	detected	1520:1527	arg2	loss					1489:1492	loss	1489:1492	loss of light chains	1489:1508	In addition, multiple other sources of micro-heterogeneity, such as glycation, lack of glycosylation, and loss of light chains, could be detected by this approach, and the contribution of multiple types of modifications to the overall micro-heterogeneity could be assessed using our superposition algorithm.
28281873	4	56	theme	complementary	926:938	arg1	analysis					940:947	complementary analysis	926:947	complementary analysis	926:947	Here, we tested a hybrid strategy for the comprehensive characterization of micro-heterogeneity by integrating 2 state-of-the-art MS-based approaches, high-resolution native MS and targeted glycan profiling, to perform complementary analysis at the intact protein level and released glycan level, respectively.
28281873	6	57	theme	composition	1261:1271	arg1	profiling					1273:1281	composition profiling	1261:1281	composition profiling	1261:1281	The glycosylation characterization data derived from these approaches were found to be mutually consistent in composition profiling, and complementary in identification and relative-quantitation of low-abundant uncommon glycoforms.
28281873	6	58	from	profiling	1273:1281	arg1	consistent					1247:1256	consistent	1247:1256	consistent	1247:1256	The glycosylation characterization data derived from these approaches were found to be mutually consistent in composition profiling, and complementary in identification and relative-quantitation of low-abundant uncommon glycoforms.
28281873	6	58	from	profiling	1273:1281	arg1	data					1186:1189	The glycosylation characterization data	1151:1189	The glycosylation characterization data derived from these approaches	1151:1219	The glycosylation characterization data derived from these approaches were found to be mutually consistent in composition profiling, and complementary in identification and relative-quantitation of low-abundant uncommon glycoforms.
28281873	5	59	theme	half-body	1091:1099	arg1	IgG4s					1101:1105	engineered half-body IgG4s	1080:1105	engineered half-body IgG4s	1080:1105	We compared the performance of these methods using samples of engineered half-body IgG4s and a panel of mAbs approved for human use.
28281873	7	60	theme	micro-heterogeneity	1422:1440	arg1	glycation					1451:1459	glycation	1451:1459	glycation	1451:1459	In addition, multiple other sources of micro-heterogeneity, such as glycation, lack of glycosylation, and loss of light chains, could be detected by this approach, and the contribution of multiple types of modifications to the overall micro-heterogeneity could be assessed using our superposition algorithm.
28281873	7	60	theme	micro-heterogeneity	1422:1440	arg1	lack					1462:1465	lack	1462:1465	lack of glycosylation	1462:1482	In addition, multiple other sources of micro-heterogeneity, such as glycation, lack of glycosylation, and loss of light chains, could be detected by this approach, and the contribution of multiple types of modifications to the overall micro-heterogeneity could be assessed using our superposition algorithm.
28281873	7	60	theme	micro-heterogeneity	1422:1440	arg1	loss					1489:1492	loss	1489:1492	loss of light chains	1489:1508	In addition, multiple other sources of micro-heterogeneity, such as glycation, lack of glycosylation, and loss of light chains, could be detected by this approach, and the contribution of multiple types of modifications to the overall micro-heterogeneity could be assessed using our superposition algorithm.
28281873	7	60	theme	micro-heterogeneity	1422:1440	arg1	sources					1411:1417	multiple other sources	1396:1417	multiple other sources	1396:1417	In addition, multiple other sources of micro-heterogeneity, such as glycation, lack of glycosylation, and loss of light chains, could be detected by this approach, and the contribution of multiple types of modifications to the overall micro-heterogeneity could be assessed using our superposition algorithm.
28281873	4	61	theme	released	981:988	arg1	level					997:1001	released glycan level	981:1001	released glycan level	981:1001	Here, we tested a hybrid strategy for the comprehensive characterization of micro-heterogeneity by integrating 2 state-of-the-art MS-based approaches, high-resolution native MS and targeted glycan profiling, to perform complementary analysis at the intact protein level and released glycan level, respectively.
28281873	0	62	theme	mass	94:97	arg1	approaches					113:122	hybrid mass spectrometric approaches	87:122	hybrid mass spectrometric approaches	87:122	Resolving the micro-heterogeneity and structural integrity of monoclonal antibodies by hybrid mass spectrometric approaches.
28281873	8	63	theme	single	1864:1869	arg1	approach					1871:1878	only a single approach	1857:1878	only a single approach	1857:1878	Our data demonstrate that the hybrid strategy allows reliable and thorough characterization of mAbs, revealing product characteristics that would easily be missed if only a single approach were used.
28281873	7	64	theme	overall	1610:1616	arg1	micro-heterogeneity					1618:1636	the overall micro-heterogeneity	1606:1636	the overall micro-heterogeneity	1606:1636	In addition, multiple other sources of micro-heterogeneity, such as glycation, lack of glycosylation, and loss of light chains, could be detected by this approach, and the contribution of multiple types of modifications to the overall micro-heterogeneity could be assessed using our superposition algorithm.
28281873	2	65	theme	mAb	572:574	arg1	profile					559:565	the proteoform profile	544:565	the proteoform profile of a mAb	544:574	Despite the recent rapid development of new bioanalytical techniques, it is still challenging to completely characterize the proteoform profile of a mAb.
28281873	1	66	theme	product	358:364	arg1	characterization					366:381	product characterization	358:381	product characterization	358:381	For therapeutic monoclonal antibodies (mAbs), detailed analysis of the structural integrity and heterogeneity, which results from multiple types of post-translational modifications (PTMs), is relevant to various processes, including product characterization, storage stability and quality control.
28281873	2	67	theme	proteoform	548:557	arg1	profile					559:565	the proteoform profile	544:565	the proteoform profile of a mAb	544:574	Despite the recent rapid development of new bioanalytical techniques, it is still challenging to completely characterize the proteoform profile of a mAb.
28281873	7	68	theme	other	1405:1409	arg1	glycation					1451:1459	glycation	1451:1459	glycation	1451:1459	In addition, multiple other sources of micro-heterogeneity, such as glycation, lack of glycosylation, and loss of light chains, could be detected by this approach, and the contribution of multiple types of modifications to the overall micro-heterogeneity could be assessed using our superposition algorithm.
28281873	7	68	theme	other	1405:1409	arg1	lack					1462:1465	lack	1462:1465	lack of glycosylation	1462:1482	In addition, multiple other sources of micro-heterogeneity, such as glycation, lack of glycosylation, and loss of light chains, could be detected by this approach, and the contribution of multiple types of modifications to the overall micro-heterogeneity could be assessed using our superposition algorithm.
28281873	7	68	theme	other	1405:1409	arg1	loss					1489:1492	loss	1489:1492	loss of light chains	1489:1508	In addition, multiple other sources of micro-heterogeneity, such as glycation, lack of glycosylation, and loss of light chains, could be detected by this approach, and the contribution of multiple types of modifications to the overall micro-heterogeneity could be assessed using our superposition algorithm.
28281873	7	68	theme	other	1405:1409	arg1	sources					1411:1417	multiple other sources	1396:1417	multiple other sources	1396:1417	In addition, multiple other sources of micro-heterogeneity, such as glycation, lack of glycosylation, and loss of light chains, could be detected by this approach, and the contribution of multiple types of modifications to the overall micro-heterogeneity could be assessed using our superposition algorithm.
28281873	4	69	theme	micro-heterogeneity	783:801	arg1	characterization					763:778	the comprehensive characterization	745:778	the comprehensive characterization of micro-heterogeneity	745:801	Here, we tested a hybrid strategy for the comprehensive characterization of micro-heterogeneity by integrating 2 state-of-the-art MS-based approaches, high-resolution native MS and targeted glycan profiling, to perform complementary analysis at the intact protein level and released glycan level, respectively.
28281873	2	70	theme	new	463:465	arg1	techniques					481:490	new bioanalytical techniques	463:490	new bioanalytical techniques	463:490	Despite the recent rapid development of new bioanalytical techniques, it is still challenging to completely characterize the proteoform profile of a mAb.
28281873	3	71	from	tool	603:606	arg1	analysis					615:622	mAb analysis	611:622	mAb analysis	611:622	As a nearly indispensable tool in mAb analysis, mass spectrometry (MS) provides unique structural information at multiple levels.
28281873	2	72	theme	recent	435:440	arg1	development					448:458	the recent rapid development	431:458	the recent rapid development of new bioanalytical techniques	431:490	Despite the recent rapid development of new bioanalytical techniques, it is still challenging to completely characterize the proteoform profile of a mAb.
28281873	7	73	theme	modifications	1589:1601	arg1	types					1580:1584	multiple types	1571:1584	multiple types of modifications	1571:1601	In addition, multiple other sources of micro-heterogeneity, such as glycation, lack of glycosylation, and loss of light chains, could be detected by this approach, and the contribution of multiple types of modifications to the overall micro-heterogeneity could be assessed using our superposition algorithm.
28281873	5	74	theme	methods	1055:1061	arg1	performance					1034:1044	the performance	1030:1044	the performance of these methods	1030:1061	We compared the performance of these methods using samples of engineered half-body IgG4s and a panel of mAbs approved for human use.
28281873	0	75	theme	monoclonal	62:71	arg1	antibodies					73:82	monoclonal antibodies	62:82	monoclonal antibodies	62:82	Resolving the micro-heterogeneity and structural integrity of monoclonal antibodies by hybrid mass spectrometric approaches.
28281873	7	76	theme	types	1580:1584	arg1	contribution					1555:1566	the contribution	1551:1566	the contribution of multiple types of modifications to the overall micro-heterogeneity	1551:1636	In addition, multiple other sources of micro-heterogeneity, such as glycation, lack of glycosylation, and loss of light chains, could be detected by this approach, and the contribution of multiple types of modifications to the overall micro-heterogeneity could be assessed using our superposition algorithm.
28281873	0	77	gly	micro-heterogeneity	14:32	arg1	antibodies					73:82	monoclonal antibodies	62:82	monoclonal antibodies	62:82	Resolving the micro-heterogeneity and structural integrity of monoclonal antibodies by hybrid mass spectrometric approaches.
28281873	5	78	theme	human	1140:1144	arg1	use					1146:1148	human use	1140:1148	human use	1140:1148	We compared the performance of these methods using samples of engineered half-body IgG4s and a panel of mAbs approved for human use.
28281873	6	79	theme	characterization	1169:1184	arg1	consistent					1247:1256	consistent	1247:1256	consistent	1247:1256	The glycosylation characterization data derived from these approaches were found to be mutually consistent in composition profiling, and complementary in identification and relative-quantitation of low-abundant uncommon glycoforms.
28281873	6	79	theme	characterization	1169:1184	arg1	data					1186:1189	The glycosylation characterization data	1151:1189	The glycosylation characterization data derived from these approaches	1151:1219	The glycosylation characterization data derived from these approaches were found to be mutually consistent in composition profiling, and complementary in identification and relative-quantitation of low-abundant uncommon glycoforms.
28281873	2	80	theme	techniques	481:490	arg1	development					448:458	the recent rapid development	431:458	the recent rapid development of new bioanalytical techniques	431:490	Despite the recent rapid development of new bioanalytical techniques, it is still challenging to completely characterize the proteoform profile of a mAb.
28281873	4	81	theme	high-resolution	858:872	arg1	MS					881:882	high-resolution native MS	858:882	high-resolution native MS	858:882	Here, we tested a hybrid strategy for the comprehensive characterization of micro-heterogeneity by integrating 2 state-of-the-art MS-based approaches, high-resolution native MS and targeted glycan profiling, to perform complementary analysis at the intact protein level and released glycan level, respectively.
28281873	4	81	theme	high-resolution	858:872	arg1	approaches					846:855	2 state-of-the-art MS-based approaches	818:855	2 state-of-the-art MS-based approaches	818:855	Here, we tested a hybrid strategy for the comprehensive characterization of micro-heterogeneity by integrating 2 state-of-the-art MS-based approaches, high-resolution native MS and targeted glycan profiling, to perform complementary analysis at the intact protein level and released glycan level, respectively.
28281873	8	82	theme	hybrid	1721:1726	arg1	strategy					1728:1735	the hybrid strategy	1717:1735	the hybrid strategy	1717:1735	Our data demonstrate that the hybrid strategy allows reliable and thorough characterization of mAbs, revealing product characteristics that would easily be missed if only a single approach were used.
28281873	1	83	theme	various	329:335	arg1	control					414:420	quality control	406:420	quality control	406:420	For therapeutic monoclonal antibodies (mAbs), detailed analysis of the structural integrity and heterogeneity, which results from multiple types of post-translational modifications (PTMs), is relevant to various processes, including product characterization, storage stability and quality control.
28281873	1	83	theme	various	329:335	arg1	processes					337:345	various processes	329:345	various processes	329:345	For therapeutic monoclonal antibodies (mAbs), detailed analysis of the structural integrity and heterogeneity, which results from multiple types of post-translational modifications (PTMs), is relevant to various processes, including product characterization, storage stability and quality control.
28281873	1	83	theme	various	329:335	arg1	characterization					366:381	product characterization	358:381	product characterization	358:381	For therapeutic monoclonal antibodies (mAbs), detailed analysis of the structural integrity and heterogeneity, which results from multiple types of post-translational modifications (PTMs), is relevant to various processes, including product characterization, storage stability and quality control.
28281873	1	83	theme	various	329:335	arg1	stability					392:400	storage stability	384:400	storage stability	384:400	For therapeutic monoclonal antibodies (mAbs), detailed analysis of the structural integrity and heterogeneity, which results from multiple types of post-translational modifications (PTMs), is relevant to various processes, including product characterization, storage stability and quality control.
28281873	3	84	theme	structural	664:673	arg1	information					675:685	unique structural information	657:685	unique structural information at multiple levels	657:704	As a nearly indispensable tool in mAb analysis, mass spectrometry (MS) provides unique structural information at multiple levels.
28281873	3	84	theme	structural	664:673	arg1	tool					603:606	a nearly indispensable tool	580:606	a nearly indispensable tool in mAb analysis	580:622	As a nearly indispensable tool in mAb analysis, mass spectrometry (MS) provides unique structural information at multiple levels.
28281873	6	85	from	relative-quantitation	1324:1344	arg1	complementary					1288:1300	complementary	1288:1300	complementary	1288:1300	The glycosylation characterization data derived from these approaches were found to be mutually consistent in composition profiling, and complementary in identification and relative-quantitation of low-abundant uncommon glycoforms.
28281873	4	86	theme	state-of-the-art	820:835	arg1	MS					881:882	high-resolution native MS	858:882	high-resolution native MS	858:882	Here, we tested a hybrid strategy for the comprehensive characterization of micro-heterogeneity by integrating 2 state-of-the-art MS-based approaches, high-resolution native MS and targeted glycan profiling, to perform complementary analysis at the intact protein level and released glycan level, respectively.
28281873	4	86	theme	state-of-the-art	820:835	arg1	profiling					904:912	targeted glycan profiling	888:912	targeted glycan profiling	888:912	Here, we tested a hybrid strategy for the comprehensive characterization of micro-heterogeneity by integrating 2 state-of-the-art MS-based approaches, high-resolution native MS and targeted glycan profiling, to perform complementary analysis at the intact protein level and released glycan level, respectively.
28281873	4	86	theme	state-of-the-art	820:835	arg1	approaches					846:855	2 state-of-the-art MS-based approaches	818:855	2 state-of-the-art MS-based approaches	818:855	Here, we tested a hybrid strategy for the comprehensive characterization of micro-heterogeneity by integrating 2 state-of-the-art MS-based approaches, high-resolution native MS and targeted glycan profiling, to perform complementary analysis at the intact protein level and released glycan level, respectively.
28281873	1	87	theme	multiple	255:262	arg1	types					264:268	multiple types	255:268	multiple types of post-translational modifications (PTMs)	255:311	For therapeutic monoclonal antibodies (mAbs), detailed analysis of the structural integrity and heterogeneity, which results from multiple types of post-translational modifications (PTMs), is relevant to various processes, including product characterization, storage stability and quality control.
26051342	6	0	theme	same	890:893	arg1	concentration					895:907	the same concentration	886:907	the same concentration	886:907	GUPs with lower molecular weight and higher ratio of glucose, basically exhibited higher antioxidant activities at the same concentration.
26051342	2	1	theme	G.	334:335	arg1	uralensis					337:345	G. uralensis	334:345	G. uralensis	334:345	In this study, three water-soluble polysaccharides fractions (GUPs-1, GUPs-2 and GUPs-3) were isolated and purified from the root of G. uralensis by DEAE-52 and Sephadex G-100 column chromatography.
26051342	0	2	theme	Glycyrrhiza	88:98	arg1	uralensis					100:108	Glycyrrhiza uralensis	88:108	Glycyrrhiza uralensis	88:108	Purification, partial characterization and antioxidant activity of polysaccharides from Glycyrrhiza uralensis.
26051342	6	3	theme	molecular	787:795	arg1	weight					797:802	lower molecular weight	781:802	lower molecular weight	781:802	GUPs with lower molecular weight and higher ratio of glucose, basically exhibited higher antioxidant activities at the same concentration.
26051342	1	4	theme	long	171:174	arg1	history					176:182	a long history	169:182	a long history of use in China	169:198	Glycyrrhiza uralensis, an important Chinese medicine, has a long history of use in China.
26051342	6	5	theme	lower	781:785	arg1	weight					797:802	lower molecular weight	781:802	lower molecular weight	781:802	GUPs with lower molecular weight and higher ratio of glucose, basically exhibited higher antioxidant activities at the same concentration.
26051342	7	6	theme	GUPs	1002:1005	arg1	composition					983:993	monosaccharide composition	968:993	monosaccharide composition of the GUPs	968:1005	This indicated that the molecular weight and the ratio of monosaccharide composition of the GUPs could affect the antioxidant activities.
26051342	6	7	theme	antioxidant	860:870	arg1	activities					872:881	higher antioxidant activities	853:881	higher antioxidant activities	853:881	GUPs with lower molecular weight and higher ratio of glucose, basically exhibited higher antioxidant activities at the same concentration.
26051342	7	8	theme	molecular	934:942	arg1	weight					944:949	the molecular weight	930:949	the molecular weight	930:949	This indicated that the molecular weight and the ratio of monosaccharide composition of the GUPs could affect the antioxidant activities.
26051342	2	9	dep	fractions	252:260	arg1	fractions					252:260	three water-soluble polysaccharides fractions	216:260	three water-soluble polysaccharides fractions (GUPs-1, GUPs-2 and GUPs-3)	216:288	In this study, three water-soluble polysaccharides fractions (GUPs-1, GUPs-2 and GUPs-3) were isolated and purified from the root of G. uralensis by DEAE-52 and Sephadex G-100 column chromatography.
26051342	2	9	dep	fractions	252:260	arg1	GUPs-3					282:287	GUPs-3	282:287	GUPs-3	282:287	In this study, three water-soluble polysaccharides fractions (GUPs-1, GUPs-2 and GUPs-3) were isolated and purified from the root of G. uralensis by DEAE-52 and Sephadex G-100 column chromatography.
26051342	2	9	dep	fractions	252:260	arg1	GUPs-2					271:276	GUPs-2	271:276	GUPs-2	271:276	In this study, three water-soluble polysaccharides fractions (GUPs-1, GUPs-2 and GUPs-3) were isolated and purified from the root of G. uralensis by DEAE-52 and Sephadex G-100 column chromatography.
26051342	2	9	dep	fractions	252:260	arg1	GUPs-1					263:268	GUPs-1	263:268	GUPs-1	263:268	In this study, three water-soluble polysaccharides fractions (GUPs-1, GUPs-2 and GUPs-3) were isolated and purified from the root of G. uralensis by DEAE-52 and Sephadex G-100 column chromatography.
26051342	1	10	contain	has	165:167	arg2	history					176:182	a long history	169:182	a long history of use in China	169:198	Glycyrrhiza uralensis, an important Chinese medicine, has a long history of use in China.
26051342	1	10	contain	has	165:167	arg1	medicine					155:162	an important Chinese medicine	134:162	an important Chinese medicine	134:162	Glycyrrhiza uralensis, an important Chinese medicine, has a long history of use in China.
26051342	1	10	contain	has	165:167	arg1	uralensis					123:131	Glycyrrhiza uralensis	111:131	Glycyrrhiza uralensis	111:131	Glycyrrhiza uralensis, an important Chinese medicine, has a long history of use in China.
26051342	5	11	theme	order	742:746	arg1	GUPs-1>GUPs-2>GUPs-3					749:768	the order: GUPs-1>GUPs-2>GUPs-3	738:768	the order: GUPs-1>GUPs-2>GUPs-3	738:768	The antioxidant activities of the three purified polysaccharides followed the order: GUPs-1>GUPs-2>GUPs-3.
26051342	2	12	theme	Sephadex	362:369	arg1	chromatography					384:397	Sephadex G-100 column chromatography	362:397	Sephadex G-100 column chromatography	362:397	In this study, three water-soluble polysaccharides fractions (GUPs-1, GUPs-2 and GUPs-3) were isolated and purified from the root of G. uralensis by DEAE-52 and Sephadex G-100 column chromatography.
26051342	1	13	theme	use	187:189	arg1	history					176:182	a long history	169:182	a long history of use in China	169:198	Glycyrrhiza uralensis, an important Chinese medicine, has a long history of use in China.
26051342	6	14	with	GUPs	771:774	arg1	weight					797:802	lower molecular weight	781:802	lower molecular weight	781:802	GUPs with lower molecular weight and higher ratio of glucose, basically exhibited higher antioxidant activities at the same concentration.
26051342	6	14	with	GUPs	771:774	arg1	ratio					815:819	higher ratio	808:819	higher ratio of glucose	808:830	GUPs with lower molecular weight and higher ratio of glucose, basically exhibited higher antioxidant activities at the same concentration.
26051342	1	15	theme	Glycyrrhiza	111:121	arg1	medicine					155:162	an important Chinese medicine	134:162	an important Chinese medicine	134:162	Glycyrrhiza uralensis, an important Chinese medicine, has a long history of use in China.
26051342	1	15	theme	Glycyrrhiza	111:121	arg1	uralensis					123:131	Glycyrrhiza uralensis	111:131	Glycyrrhiza uralensis	111:131	Glycyrrhiza uralensis, an important Chinese medicine, has a long history of use in China.
26051342	4	16	theme	GUPs-3	555:560	arg1	10,160					567:572	10,160	567:572	10,160	567:572	The molecular weights of GUPs-1, GUPs-2 and GUPs-3 were 10,160, 11,680 and 13,360 Da, and the ratios of glucose were 23.4%, 14% and 1.13%, respectively.
26051342	4	16	theme	GUPs-3	555:560	arg1	weights					525:531	The molecular weights	511:531	The molecular weights of GUPs-1, GUPs-2 and GUPs-3	511:560	The molecular weights of GUPs-1, GUPs-2 and GUPs-3 were 10,160, 11,680 and 13,360 Da, and the ratios of glucose were 23.4%, 14% and 1.13%, respectively.
26051342	4	17	theme	glucose	615:621	arg1	%					632:632	23.4%	628:632	23.4%	628:632	The molecular weights of GUPs-1, GUPs-2 and GUPs-3 were 10,160, 11,680 and 13,360 Da, and the ratios of glucose were 23.4%, 14% and 1.13%, respectively.
26051342	4	17	theme	glucose	615:621	arg1	ratios					605:610	the ratios	601:610	the ratios of glucose	601:621	The molecular weights of GUPs-1, GUPs-2 and GUPs-3 were 10,160, 11,680 and 13,360 Da, and the ratios of glucose were 23.4%, 14% and 1.13%, respectively.
26051342	0	18	theme	partial	14:20	arg1	characterization					22:37	partial characterization	14:37	partial characterization	14:37	Purification, partial characterization and antioxidant activity of polysaccharides from Glycyrrhiza uralensis.
26051342	0	19	from	uralensis	100:108	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification, partial characterization and antioxidant activity of polysaccharides from Glycyrrhiza uralensis.
26051342	0	19	from	uralensis	100:108	arg1	characterization					22:37	partial characterization	14:37	partial characterization	14:37	Purification, partial characterization and antioxidant activity of polysaccharides from Glycyrrhiza uralensis.
26051342	0	19	from	uralensis	100:108	arg1	activity					55:62	antioxidant activity	43:62	antioxidant activity	43:62	Purification, partial characterization and antioxidant activity of polysaccharides from Glycyrrhiza uralensis.
26051342	3	20	theme	purified	467:474	arg1	polysaccharides					476:490	the three purified polysaccharides	457:490	the three purified polysaccharides	457:490	Physicochemical properties and antioxidant activities of the three purified polysaccharides were investigated.
26051342	0	21	theme	antioxidant	43:53	arg1	activity					55:62	antioxidant activity	43:62	antioxidant activity	43:62	Purification, partial characterization and antioxidant activity of polysaccharides from Glycyrrhiza uralensis.
26051342	2	22	theme	water-soluble	222:234	arg1	fractions					252:260	three water-soluble polysaccharides fractions	216:260	three water-soluble polysaccharides fractions (GUPs-1, GUPs-2 and GUPs-3)	216:288	In this study, three water-soluble polysaccharides fractions (GUPs-1, GUPs-2 and GUPs-3) were isolated and purified from the root of G. uralensis by DEAE-52 and Sephadex G-100 column chromatography.
26051342	2	22	theme	water-soluble	222:234	arg1	GUPs-3					282:287	GUPs-3	282:287	GUPs-3	282:287	In this study, three water-soluble polysaccharides fractions (GUPs-1, GUPs-2 and GUPs-3) were isolated and purified from the root of G. uralensis by DEAE-52 and Sephadex G-100 column chromatography.
26051342	2	22	theme	water-soluble	222:234	arg1	GUPs-2					271:276	GUPs-2	271:276	GUPs-2	271:276	In this study, three water-soluble polysaccharides fractions (GUPs-1, GUPs-2 and GUPs-3) were isolated and purified from the root of G. uralensis by DEAE-52 and Sephadex G-100 column chromatography.
26051342	2	22	theme	water-soluble	222:234	arg1	GUPs-1					263:268	GUPs-1	263:268	GUPs-1	263:268	In this study, three water-soluble polysaccharides fractions (GUPs-1, GUPs-2 and GUPs-3) were isolated and purified from the root of G. uralensis by DEAE-52 and Sephadex G-100 column chromatography.
26051342	2	23	theme	G-100	371:375	arg1	chromatography					384:397	Sephadex G-100 column chromatography	362:397	Sephadex G-100 column chromatography	362:397	In this study, three water-soluble polysaccharides fractions (GUPs-1, GUPs-2 and GUPs-3) were isolated and purified from the root of G. uralensis by DEAE-52 and Sephadex G-100 column chromatography.
26051342	3	24	theme	Physicochemical	400:414	arg1	properties					416:425	Physicochemical properties	400:425	Physicochemical properties	400:425	Physicochemical properties and antioxidant activities of the three purified polysaccharides were investigated.
26051342	6	25	theme	glucose	824:830	arg1	weight					797:802	lower molecular weight	781:802	lower molecular weight	781:802	GUPs with lower molecular weight and higher ratio of glucose, basically exhibited higher antioxidant activities at the same concentration.
26051342	6	25	theme	glucose	824:830	arg1	ratio					815:819	higher ratio	808:819	higher ratio of glucose	808:830	GUPs with lower molecular weight and higher ratio of glucose, basically exhibited higher antioxidant activities at the same concentration.
26051342	2	26	theme	column	377:382	arg1	chromatography					384:397	Sephadex G-100 column chromatography	362:397	Sephadex G-100 column chromatography	362:397	In this study, three water-soluble polysaccharides fractions (GUPs-1, GUPs-2 and GUPs-3) were isolated and purified from the root of G. uralensis by DEAE-52 and Sephadex G-100 column chromatography.
26051342	4	27	theme	GUPs-1	536:541	arg1	10,160					567:572	10,160	567:572	10,160	567:572	The molecular weights of GUPs-1, GUPs-2 and GUPs-3 were 10,160, 11,680 and 13,360 Da, and the ratios of glucose were 23.4%, 14% and 1.13%, respectively.
26051342	4	27	theme	GUPs-1	536:541	arg1	weights					525:531	The molecular weights	511:531	The molecular weights of GUPs-1, GUPs-2 and GUPs-3	511:560	The molecular weights of GUPs-1, GUPs-2 and GUPs-3 were 10,160, 11,680 and 13,360 Da, and the ratios of glucose were 23.4%, 14% and 1.13%, respectively.
26051342	3	28	theme	antioxidant	431:441	arg1	activities					443:452	antioxidant activities	431:452	antioxidant activities	431:452	Physicochemical properties and antioxidant activities of the three purified polysaccharides were investigated.
26051342	6	29	theme	higher	853:858	arg1	activities					872:881	higher antioxidant activities	853:881	higher antioxidant activities	853:881	GUPs with lower molecular weight and higher ratio of glucose, basically exhibited higher antioxidant activities at the same concentration.
26051342	5	30	theme	antioxidant	668:678	arg1	activities					680:689	The antioxidant activities	664:689	The antioxidant activities of the three purified polysaccharides	664:727	The antioxidant activities of the three purified polysaccharides followed the order: GUPs-1>GUPs-2>GUPs-3.
26051342	7	31	theme	antioxidant	1024:1034	arg1	activities					1036:1045	the antioxidant activities	1020:1045	the antioxidant activities	1020:1045	This indicated that the molecular weight and the ratio of monosaccharide composition of the GUPs could affect the antioxidant activities.
26051342	7	32	theme	composition	983:993	arg1	weight					944:949	the molecular weight	930:949	the molecular weight	930:949	This indicated that the molecular weight and the ratio of monosaccharide composition of the GUPs could affect the antioxidant activities.
26051342	7	32	theme	composition	983:993	arg1	ratio					959:963	the ratio	955:963	the ratio of monosaccharide composition of the GUPs	955:1005	This indicated that the molecular weight and the ratio of monosaccharide composition of the GUPs could affect the antioxidant activities.
26051342	1	33	theme	important	137:145	arg1	medicine					155:162	an important Chinese medicine	134:162	an important Chinese medicine	134:162	Glycyrrhiza uralensis, an important Chinese medicine, has a long history of use in China.
26051342	1	33	theme	important	137:145	arg1	uralensis					123:131	Glycyrrhiza uralensis	111:131	Glycyrrhiza uralensis	111:131	Glycyrrhiza uralensis, an important Chinese medicine, has a long history of use in China.
26051342	4	34	theme	GUPs-2	544:549	arg1	10,160					567:572	10,160	567:572	10,160	567:572	The molecular weights of GUPs-1, GUPs-2 and GUPs-3 were 10,160, 11,680 and 13,360 Da, and the ratios of glucose were 23.4%, 14% and 1.13%, respectively.
26051342	4	34	theme	GUPs-2	544:549	arg1	weights					525:531	The molecular weights	511:531	The molecular weights of GUPs-1, GUPs-2 and GUPs-3	511:560	The molecular weights of GUPs-1, GUPs-2 and GUPs-3 were 10,160, 11,680 and 13,360 Da, and the ratios of glucose were 23.4%, 14% and 1.13%, respectively.
26051342	6	35	theme	higher	808:813	arg1	ratio					815:819	higher ratio	808:819	higher ratio of glucose	808:830	GUPs with lower molecular weight and higher ratio of glucose, basically exhibited higher antioxidant activities at the same concentration.
26051342	5	36	theme	polysaccharides	713:727	arg1	activities					680:689	The antioxidant activities	664:689	The antioxidant activities of the three purified polysaccharides	664:727	The antioxidant activities of the three purified polysaccharides followed the order: GUPs-1>GUPs-2>GUPs-3.
26051342	1	37	theme	Chinese	147:153	arg1	medicine					155:162	an important Chinese medicine	134:162	an important Chinese medicine	134:162	Glycyrrhiza uralensis, an important Chinese medicine, has a long history of use in China.
26051342	1	37	theme	Chinese	147:153	arg1	uralensis					123:131	Glycyrrhiza uralensis	111:131	Glycyrrhiza uralensis	111:131	Glycyrrhiza uralensis, an important Chinese medicine, has a long history of use in China.
26051342	3	38	theme	polysaccharides	476:490	arg1	properties					416:425	Physicochemical properties	400:425	Physicochemical properties	400:425	Physicochemical properties and antioxidant activities of the three purified polysaccharides were investigated.
26051342	3	38	theme	polysaccharides	476:490	arg1	activities					443:452	antioxidant activities	431:452	antioxidant activities	431:452	Physicochemical properties and antioxidant activities of the three purified polysaccharides were investigated.
26051342	5	39	theme	purified	704:711	arg1	polysaccharides					713:727	the three purified polysaccharides	694:727	the three purified polysaccharides	694:727	The antioxidant activities of the three purified polysaccharides followed the order: GUPs-1>GUPs-2>GUPs-3.
26051342	7	40	theme	monosaccharide	968:981	arg1	composition					983:993	monosaccharide composition	968:993	monosaccharide composition of the GUPs	968:1005	This indicated that the molecular weight and the ratio of monosaccharide composition of the GUPs could affect the antioxidant activities.
26051342	0	41	theme	polysaccharides	67:81	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification, partial characterization and antioxidant activity of polysaccharides from Glycyrrhiza uralensis.
26051342	0	41	theme	polysaccharides	67:81	arg1	characterization					22:37	partial characterization	14:37	partial characterization	14:37	Purification, partial characterization and antioxidant activity of polysaccharides from Glycyrrhiza uralensis.
26051342	0	41	theme	polysaccharides	67:81	arg1	activity					55:62	antioxidant activity	43:62	antioxidant activity	43:62	Purification, partial characterization and antioxidant activity of polysaccharides from Glycyrrhiza uralensis.
26051342	4	42	theme	molecular	515:523	arg1	10,160					567:572	10,160	567:572	10,160	567:572	The molecular weights of GUPs-1, GUPs-2 and GUPs-3 were 10,160, 11,680 and 13,360 Da, and the ratios of glucose were 23.4%, 14% and 1.13%, respectively.
26051342	4	42	theme	molecular	515:523	arg1	weights					525:531	The molecular weights	511:531	The molecular weights of GUPs-1, GUPs-2 and GUPs-3	511:560	The molecular weights of GUPs-1, GUPs-2 and GUPs-3 were 10,160, 11,680 and 13,360 Da, and the ratios of glucose were 23.4%, 14% and 1.13%, respectively.
26051342	2	43	theme	polysaccharides	236:250	arg1	fractions					252:260	three water-soluble polysaccharides fractions	216:260	three water-soluble polysaccharides fractions (GUPs-1, GUPs-2 and GUPs-3)	216:288	In this study, three water-soluble polysaccharides fractions (GUPs-1, GUPs-2 and GUPs-3) were isolated and purified from the root of G. uralensis by DEAE-52 and Sephadex G-100 column chromatography.
26051342	2	43	theme	polysaccharides	236:250	arg1	GUPs-3					282:287	GUPs-3	282:287	GUPs-3	282:287	In this study, three water-soluble polysaccharides fractions (GUPs-1, GUPs-2 and GUPs-3) were isolated and purified from the root of G. uralensis by DEAE-52 and Sephadex G-100 column chromatography.
26051342	2	43	theme	polysaccharides	236:250	arg1	GUPs-2					271:276	GUPs-2	271:276	GUPs-2	271:276	In this study, three water-soluble polysaccharides fractions (GUPs-1, GUPs-2 and GUPs-3) were isolated and purified from the root of G. uralensis by DEAE-52 and Sephadex G-100 column chromatography.
26051342	2	43	theme	polysaccharides	236:250	arg1	GUPs-1					263:268	GUPs-1	263:268	GUPs-1	263:268	In this study, three water-soluble polysaccharides fractions (GUPs-1, GUPs-2 and GUPs-3) were isolated and purified from the root of G. uralensis by DEAE-52 and Sephadex G-100 column chromatography.
26051342	1	44	from	history	176:182	arg1	China					194:198	China	194:198	China	194:198	Glycyrrhiza uralensis, an important Chinese medicine, has a long history of use in China.
26051342	2	45	theme	uralensis	337:345	arg1	root					326:329	the root	322:329	the root of G. uralensis	322:345	In this study, three water-soluble polysaccharides fractions (GUPs-1, GUPs-2 and GUPs-3) were isolated and purified from the root of G. uralensis by DEAE-52 and Sephadex G-100 column chromatography.
27112883	0	0	theme	Agaricus	94:101	arg1	fucogalactan					112:123	Agaricus bisporus fucogalactan	94:123	Agaricus bisporus fucogalactan	94:123	Optimization of chemical sulfation, structural characterization and anticoagulant activity of Agaricus bisporus fucogalactan.
27112883	9	1	theme	α-d-Galp	1421:1428	arg1	units					1430:1434	α-d-Galp units	1421:1434	α-d-Galp units of the main chain	1421:1452	NMR and methylation analyses showed that α-d-Galp units of the main chain were greatly sulfated on 2-O-, 3-O-, and 4-O-positions.
27112883	6	2	theme	Thromboplastin	914:927	arg1	APTT					935:938	APTT	935:938	APTT	935:938	The sulfated fucogalactan with the highest DS value (2.83) had the best anticoagulant activity on Activated Partial Thromboplastin Time (APTT) and Protrombin Time (PT) assays.
27112883	6	2	theme	Thromboplastin	914:927	arg1	Time					929:932	Activated Partial Thromboplastin Time	896:932	Activated Partial Thromboplastin Time (APTT)	896:939	The sulfated fucogalactan with the highest DS value (2.83) had the best anticoagulant activity on Activated Partial Thromboplastin Time (APTT) and Protrombin Time (PT) assays.
27112883	3	3	theme	1.28×10	475:481	arg1	gmol					485:488	1.28×10(4)gmol(-1)	475:492	1.28×10(4)gmol(-1)	475:492	HPSEC analysis showed it had Mw of 1.28×10(4)gmol(-1).
27112883	7	4	theme	ηClSO3H/OH	1058:1067	arg1	ratio					1069:1073	ηClSO3H/OH ratio	1058:1073	ηClSO3H/OH ratio of 18, VT/w ratio of 100	1058:1098	This sulfated fucogalactan, named E100, was obtained with the optimal conditions of ηClSO3H/OH ratio of 18, VT/w ratio of 100, in 6h of reaction.
27112883	4	5	theme	sulfation	567:575	arg1	agent					577:581	sulfation agent	567:581	sulfation agent	567:581	The polysaccharide was sulfated modifying reaction time, molar ratio of sulfation agent to hydroxyl group on the polysaccharide (ηClSO3H/OH ratio), and ratio of total reaction volume to weight of sample (VT/w ratio; μLmg(-1)).
27112883	1	6	theme	aqueous	163:169	arg1	extract					171:177	aqueous extract	163:177	aqueous extract of Agaricus bisporus	163:198	A fucogalactan (E) was isolated from aqueous extract of Agaricus bisporus.
27112883	7	7	theme	100	1096:1098	arg1	ratio					1087:1091	18, VT/w ratio	1078:1091	18, VT/w ratio of 100	1078:1098	This sulfated fucogalactan, named E100, was obtained with the optimal conditions of ηClSO3H/OH ratio of 18, VT/w ratio of 100, in 6h of reaction.
27112883	0	8	theme	fucogalactan	112:123	arg1	sulfation					25:33	chemical sulfation	16:33	chemical sulfation	16:33	Optimization of chemical sulfation, structural characterization and anticoagulant activity of Agaricus bisporus fucogalactan.
27112883	0	8	theme	fucogalactan	112:123	arg1	characterization					47:62	structural characterization	36:62	structural characterization	36:62	Optimization of chemical sulfation, structural characterization and anticoagulant activity of Agaricus bisporus fucogalactan.
27112883	0	8	theme	fucogalactan	112:123	arg1	activity					82:89	anticoagulant activity	68:89	anticoagulant activity	68:89	Optimization of chemical sulfation, structural characterization and anticoagulant activity of Agaricus bisporus fucogalactan.
27112883	8	9	theme	intrinsic	1340:1348	arg1	pathway					1350:1356	the intrinsic pathway	1336:1356	the intrinsic pathway of blood coagulation	1336:1377	The results showed that E100 produces a linear increment of APTT for concentrations of 15-45μgmL(-1), whereas PT was almost constant between 20 and 400μgmL(-1), suggesting an anticoagulant activity via inhibition of the intrinsic pathway of blood coagulation.
27112883	8	10	theme	APTT	1180:1183	arg1	increment					1167:1175	a linear increment	1158:1175	a linear increment of APTT	1158:1183	The results showed that E100 produces a linear increment of APTT for concentrations of 15-45μgmL(-1), whereas PT was almost constant between 20 and 400μgmL(-1), suggesting an anticoagulant activity via inhibition of the intrinsic pathway of blood coagulation.
27112883	0	11	theme	bisporus	103:110	arg1	fucogalactan					112:123	Agaricus bisporus fucogalactan	94:123	Agaricus bisporus fucogalactan	94:123	Optimization of chemical sulfation, structural characterization and anticoagulant activity of Agaricus bisporus fucogalactan.
27112883	1	12	attach	isolated	149:156	arg2	fucogalactan					128:139	A fucogalactan	126:139	A fucogalactan (E)	126:143	A fucogalactan (E) was isolated from aqueous extract of Agaricus bisporus.
27112883	1	12	attach	isolated	149:156	arg1	extract					171:177	aqueous extract	163:177	aqueous extract of Agaricus bisporus	163:198	A fucogalactan (E) was isolated from aqueous extract of Agaricus bisporus.
27112883	1	12	attach	isolated	149:156	arg2	E					142:142	E	142:142	E	142:142	A fucogalactan (E) was isolated from aqueous extract of Agaricus bisporus.
27112883	4	13	theme	reaction	537:544	arg1	time					546:549	reaction time	537:549	reaction time	537:549	The polysaccharide was sulfated modifying reaction time, molar ratio of sulfation agent to hydroxyl group on the polysaccharide (ηClSO3H/OH ratio), and ratio of total reaction volume to weight of sample (VT/w ratio; μLmg(-1)).
27112883	9	14	theme	main	1443:1446	arg1	chain					1448:1452	the main chain	1439:1452	the main chain	1439:1452	NMR and methylation analyses showed that α-d-Galp units of the main chain were greatly sulfated on 2-O-, 3-O-, and 4-O-positions.
27112883	4	15	theme	reaction	662:669	arg1	volume					671:676	total reaction volume	656:676	total reaction volume	656:676	The polysaccharide was sulfated modifying reaction time, molar ratio of sulfation agent to hydroxyl group on the polysaccharide (ηClSO3H/OH ratio), and ratio of total reaction volume to weight of sample (VT/w ratio; μLmg(-1)).
27112883	3	16	theme	gmol	485:488	arg1	Mw					469:470	Mw	469:470	Mw of 1.28×10(4)gmol(-1)	469:492	HPSEC analysis showed it had Mw of 1.28×10(4)gmol(-1).
27112883	6	17	with	fucogalactan	811:822	arg1	2.83					851:854	2.83	851:854	2.83	851:854	The sulfated fucogalactan with the highest DS value (2.83) had the best anticoagulant activity on Activated Partial Thromboplastin Time (APTT) and Protrombin Time (PT) assays.
27112883	6	17	with	fucogalactan	811:822	arg1	value					844:848	the highest DS value	829:848	the highest DS value (2.83)	829:855	The sulfated fucogalactan with the highest DS value (2.83) had the best anticoagulant activity on Activated Partial Thromboplastin Time (APTT) and Protrombin Time (PT) assays.
27112883	5	18	theme	substitution	736:747	arg1	degree					726:731	The degree	722:731	The degree of substitution (DS)	722:752	The degree of substitution (DS) was evaluated for all sulfated derivatives.
27112883	9	19	theme	chain	1448:1452	arg1	units					1430:1434	α-d-Galp units	1421:1434	α-d-Galp units of the main chain	1421:1452	NMR and methylation analyses showed that α-d-Galp units of the main chain were greatly sulfated on 2-O-, 3-O-, and 4-O-positions.
27112883	4	20	theme	sample	691:696	arg1	weight					681:686	weight	681:686	weight of sample	681:696	The polysaccharide was sulfated modifying reaction time, molar ratio of sulfation agent to hydroxyl group on the polysaccharide (ηClSO3H/OH ratio), and ratio of total reaction volume to weight of sample (VT/w ratio; μLmg(-1)).
27112883	6	21	theme	Protrombin	945:954	arg1	PT					962:963	PT	962:963	PT	962:963	The sulfated fucogalactan with the highest DS value (2.83) had the best anticoagulant activity on Activated Partial Thromboplastin Time (APTT) and Protrombin Time (PT) assays.
27112883	6	21	theme	Protrombin	945:954	arg1	Time					956:959	Protrombin Time	945:959	Protrombin Time (PT)	945:964	The sulfated fucogalactan with the highest DS value (2.83) had the best anticoagulant activity on Activated Partial Thromboplastin Time (APTT) and Protrombin Time (PT) assays.
27112883	8	22	theme	blood	1361:1365	arg1	coagulation					1367:1377	blood coagulation	1361:1377	blood coagulation	1361:1377	The results showed that E100 produces a linear increment of APTT for concentrations of 15-45μgmL(-1), whereas PT was almost constant between 20 and 400μgmL(-1), suggesting an anticoagulant activity via inhibition of the intrinsic pathway of blood coagulation.
27112883	2	23	theme	α-d-Galp	430:437	arg1	end-units					405:413	non-reducing end-units	392:413	non-reducing end-units of α-l-Fucp or α-d-Galp	392:437	The monosaccharide composition, methylation, and NMR analyses showed it is constituted by a (1→6)-linked α-d-Galp main-chain, partially methylated at O-3, and partially substituted at O-2 by non-reducing end-units of α-l-Fucp or α-d-Galp.
27112883	8	24	theme	anticoagulant	1295:1307	arg1	activity					1309:1316	an anticoagulant activity	1292:1316	an anticoagulant activity	1292:1316	The results showed that E100 produces a linear increment of APTT for concentrations of 15-45μgmL(-1), whereas PT was almost constant between 20 and 400μgmL(-1), suggesting an anticoagulant activity via inhibition of the intrinsic pathway of blood coagulation.
27112883	7	25	theme	ratio	1069:1073	arg1	conditions					1044:1053	the optimal conditions	1032:1053	the optimal conditions of ηClSO3H/OH ratio of 18, VT/w ratio of 100	1032:1098	This sulfated fucogalactan, named E100, was obtained with the optimal conditions of ηClSO3H/OH ratio of 18, VT/w ratio of 100, in 6h of reaction.
27112883	2	26	theme	-linked	298:304	arg1	main-chain					315:324	a (1→6)-linked α-d-Galp main-chain	291:324	a (1→6)-linked α-d-Galp main-chain	291:324	The monosaccharide composition, methylation, and NMR analyses showed it is constituted by a (1→6)-linked α-d-Galp main-chain, partially methylated at O-3, and partially substituted at O-2 by non-reducing end-units of α-l-Fucp or α-d-Galp.
27112883	4	27	from	group	595:599	arg1	ratio					635:639	ηClSO3H/OH ratio	624:639	ηClSO3H/OH ratio	624:639	The polysaccharide was sulfated modifying reaction time, molar ratio of sulfation agent to hydroxyl group on the polysaccharide (ηClSO3H/OH ratio), and ratio of total reaction volume to weight of sample (VT/w ratio; μLmg(-1)).
27112883	4	27	from	group	595:599	arg1	polysaccharide					608:621	the polysaccharide	604:621	the polysaccharide (ηClSO3H/OH ratio)	604:640	The polysaccharide was sulfated modifying reaction time, molar ratio of sulfation agent to hydroxyl group on the polysaccharide (ηClSO3H/OH ratio), and ratio of total reaction volume to weight of sample (VT/w ratio; μLmg(-1)).
27112883	4	28	dep	ratio	704:708	arg1	-1					716:717	-1	716:717	-1	716:717	The polysaccharide was sulfated modifying reaction time, molar ratio of sulfation agent to hydroxyl group on the polysaccharide (ηClSO3H/OH ratio), and ratio of total reaction volume to weight of sample (VT/w ratio; μLmg(-1)).
27112883	4	28	dep	ratio	704:708	arg1	μLmg					711:714	μLmg	711:714	VT/w ratio; μLmg	699:714	The polysaccharide was sulfated modifying reaction time, molar ratio of sulfation agent to hydroxyl group on the polysaccharide (ηClSO3H/OH ratio), and ratio of total reaction volume to weight of sample (VT/w ratio; μLmg(-1)).
27112883	6	29	theme	sulfated	802:809	arg1	fucogalactan					811:822	The sulfated fucogalactan	798:822	The sulfated fucogalactan with the highest DS value (2.83)	798:855	The sulfated fucogalactan with the highest DS value (2.83) had the best anticoagulant activity on Activated Partial Thromboplastin Time (APTT) and Protrombin Time (PT) assays.
27112883	4	30	theme	total	656:660	arg1	volume					671:676	total reaction volume	656:676	total reaction volume	656:676	The polysaccharide was sulfated modifying reaction time, molar ratio of sulfation agent to hydroxyl group on the polysaccharide (ηClSO3H/OH ratio), and ratio of total reaction volume to weight of sample (VT/w ratio; μLmg(-1)).
27112883	8	31	theme	linear	1160:1165	arg1	increment					1167:1175	a linear increment	1158:1175	a linear increment of APTT	1158:1183	The results showed that E100 produces a linear increment of APTT for concentrations of 15-45μgmL(-1), whereas PT was almost constant between 20 and 400μgmL(-1), suggesting an anticoagulant activity via inhibition of the intrinsic pathway of blood coagulation.
27112883	1	32	theme	bisporus	191:198	arg1	extract					171:177	aqueous extract	163:177	aqueous extract of Agaricus bisporus	163:198	A fucogalactan (E) was isolated from aqueous extract of Agaricus bisporus.
27112883	0	33	theme	sulfation	25:33	arg1	Optimization					0:11	Optimization	0:11	Optimization of chemical sulfation, structural characterization and anticoagulant activity of Agaricus bisporus fucogalactan	0:123	Optimization of chemical sulfation, structural characterization and anticoagulant activity of Agaricus bisporus fucogalactan.
27112883	6	34	contain	had	857:859	arg2	activity					884:891	the best anticoagulant activity	861:891	the best anticoagulant activity	861:891	The sulfated fucogalactan with the highest DS value (2.83) had the best anticoagulant activity on Activated Partial Thromboplastin Time (APTT) and Protrombin Time (PT) assays.
27112883	6	34	contain	had	857:859	arg1	fucogalactan					811:822	The sulfated fucogalactan	798:822	The sulfated fucogalactan with the highest DS value (2.83)	798:855	The sulfated fucogalactan with the highest DS value (2.83) had the best anticoagulant activity on Activated Partial Thromboplastin Time (APTT) and Protrombin Time (PT) assays.
27112883	4	35	theme	agent	577:581	arg1	ratio					558:562	molar ratio	552:562	molar ratio of sulfation agent to hydroxyl group on the polysaccharide (ηClSO3H/OH ratio)	552:640	The polysaccharide was sulfated modifying reaction time, molar ratio of sulfation agent to hydroxyl group on the polysaccharide (ηClSO3H/OH ratio), and ratio of total reaction volume to weight of sample (VT/w ratio; μLmg(-1)).
27112883	4	35	theme	agent	577:581	arg1	time					546:549	reaction time	537:549	reaction time	537:549	The polysaccharide was sulfated modifying reaction time, molar ratio of sulfation agent to hydroxyl group on the polysaccharide (ηClSO3H/OH ratio), and ratio of total reaction volume to weight of sample (VT/w ratio; μLmg(-1)).
27112883	4	35	theme	agent	577:581	arg1	ratio					647:651	ratio	647:651	ratio of total reaction volume	647:676	The polysaccharide was sulfated modifying reaction time, molar ratio of sulfation agent to hydroxyl group on the polysaccharide (ηClSO3H/OH ratio), and ratio of total reaction volume to weight of sample (VT/w ratio; μLmg(-1)).
27112883	0	36	theme	chemical	16:23	arg1	sulfation					25:33	chemical sulfation	16:33	chemical sulfation	16:33	Optimization of chemical sulfation, structural characterization and anticoagulant activity of Agaricus bisporus fucogalactan.
27112883	6	37	theme	DS	841:842	arg1	2.83					851:854	2.83	851:854	2.83	851:854	The sulfated fucogalactan with the highest DS value (2.83) had the best anticoagulant activity on Activated Partial Thromboplastin Time (APTT) and Protrombin Time (PT) assays.
27112883	6	37	theme	DS	841:842	arg1	value					844:848	the highest DS value	829:848	the highest DS value (2.83)	829:855	The sulfated fucogalactan with the highest DS value (2.83) had the best anticoagulant activity on Activated Partial Thromboplastin Time (APTT) and Protrombin Time (PT) assays.
27112883	6	38	dep	Time	929:932	arg1	assays					966:971	assays	966:971	assays	966:971	The sulfated fucogalactan with the highest DS value (2.83) had the best anticoagulant activity on Activated Partial Thromboplastin Time (APTT) and Protrombin Time (PT) assays.
27112883	4	39	theme	volume	671:676	arg1	ratio					558:562	molar ratio	552:562	molar ratio of sulfation agent to hydroxyl group on the polysaccharide (ηClSO3H/OH ratio)	552:640	The polysaccharide was sulfated modifying reaction time, molar ratio of sulfation agent to hydroxyl group on the polysaccharide (ηClSO3H/OH ratio), and ratio of total reaction volume to weight of sample (VT/w ratio; μLmg(-1)).
27112883	4	39	theme	volume	671:676	arg1	time					546:549	reaction time	537:549	reaction time	537:549	The polysaccharide was sulfated modifying reaction time, molar ratio of sulfation agent to hydroxyl group on the polysaccharide (ηClSO3H/OH ratio), and ratio of total reaction volume to weight of sample (VT/w ratio; μLmg(-1)).
27112883	4	39	theme	volume	671:676	arg1	ratio					647:651	ratio	647:651	ratio of total reaction volume	647:676	The polysaccharide was sulfated modifying reaction time, molar ratio of sulfation agent to hydroxyl group on the polysaccharide (ηClSO3H/OH ratio), and ratio of total reaction volume to weight of sample (VT/w ratio; μLmg(-1)).
27112883	2	40	theme	non-reducing	392:403	arg1	end-units					405:413	non-reducing end-units	392:413	non-reducing end-units of α-l-Fucp or α-d-Galp	392:437	The monosaccharide composition, methylation, and NMR analyses showed it is constituted by a (1→6)-linked α-d-Galp main-chain, partially methylated at O-3, and partially substituted at O-2 by non-reducing end-units of α-l-Fucp or α-d-Galp.
27112883	0	41	theme	structural	36:45	arg1	characterization					47:62	structural characterization	36:62	structural characterization	36:62	Optimization of chemical sulfation, structural characterization and anticoagulant activity of Agaricus bisporus fucogalactan.
27112883	6	42	theme	highest	833:839	arg1	2.83					851:854	2.83	851:854	2.83	851:854	The sulfated fucogalactan with the highest DS value (2.83) had the best anticoagulant activity on Activated Partial Thromboplastin Time (APTT) and Protrombin Time (PT) assays.
27112883	6	42	theme	highest	833:839	arg1	value					844:848	the highest DS value	829:848	the highest DS value (2.83)	829:855	The sulfated fucogalactan with the highest DS value (2.83) had the best anticoagulant activity on Activated Partial Thromboplastin Time (APTT) and Protrombin Time (PT) assays.
27112883	6	43	theme	Partial	906:912	arg1	APTT					935:938	APTT	935:938	APTT	935:938	The sulfated fucogalactan with the highest DS value (2.83) had the best anticoagulant activity on Activated Partial Thromboplastin Time (APTT) and Protrombin Time (PT) assays.
27112883	6	43	theme	Partial	906:912	arg1	Time					929:932	Activated Partial Thromboplastin Time	896:932	Activated Partial Thromboplastin Time (APTT)	896:939	The sulfated fucogalactan with the highest DS value (2.83) had the best anticoagulant activity on Activated Partial Thromboplastin Time (APTT) and Protrombin Time (PT) assays.
27112883	4	44	theme	hydroxyl	586:593	arg1	group					595:599	hydroxyl group	586:599	hydroxyl group on the polysaccharide (ηClSO3H/OH ratio)	586:640	The polysaccharide was sulfated modifying reaction time, molar ratio of sulfation agent to hydroxyl group on the polysaccharide (ηClSO3H/OH ratio), and ratio of total reaction volume to weight of sample (VT/w ratio; μLmg(-1)).
27112883	8	45	theme	pathway	1350:1356	arg1	inhibition					1322:1331	inhibition	1322:1331	inhibition of the intrinsic pathway of blood coagulation	1322:1377	The results showed that E100 produces a linear increment of APTT for concentrations of 15-45μgmL(-1), whereas PT was almost constant between 20 and 400μgmL(-1), suggesting an anticoagulant activity via inhibition of the intrinsic pathway of blood coagulation.
27112883	4	46	dep	modifying	527:535	arg1	ratio					704:708	VT/w ratio	699:708	VT/w ratio; μLmg	699:714	The polysaccharide was sulfated modifying reaction time, molar ratio of sulfation agent to hydroxyl group on the polysaccharide (ηClSO3H/OH ratio), and ratio of total reaction volume to weight of sample (VT/w ratio; μLmg(-1)).
27112883	2	47	theme	α-l-Fucp	418:425	arg1	end-units					405:413	non-reducing end-units	392:413	non-reducing end-units of α-l-Fucp or α-d-Galp	392:437	The monosaccharide composition, methylation, and NMR analyses showed it is constituted by a (1→6)-linked α-d-Galp main-chain, partially methylated at O-3, and partially substituted at O-2 by non-reducing end-units of α-l-Fucp or α-d-Galp.
27112883	3	48	theme	HPSEC	440:444	arg1	analysis					446:453	HPSEC analysis	440:453	HPSEC analysis	440:453	HPSEC analysis showed it had Mw of 1.28×10(4)gmol(-1).
27112883	7	49	theme	sulfated	979:986	arg1	fucogalactan					988:999	This sulfated fucogalactan	974:999	This sulfated fucogalactan	974:999	This sulfated fucogalactan, named E100, was obtained with the optimal conditions of ηClSO3H/OH ratio of 18, VT/w ratio of 100, in 6h of reaction.
27112883	7	50	theme	optimal	1036:1042	arg1	conditions					1044:1053	the optimal conditions	1032:1053	the optimal conditions of ηClSO3H/OH ratio of 18, VT/w ratio of 100	1032:1098	This sulfated fucogalactan, named E100, was obtained with the optimal conditions of ηClSO3H/OH ratio of 18, VT/w ratio of 100, in 6h of reaction.
27112883	2	51	theme	methylation	233:243	arg1	analyses					254:261	The monosaccharide composition, methylation, and NMR analyses	201:261	The monosaccharide composition, methylation, and NMR analyses	201:261	The monosaccharide composition, methylation, and NMR analyses showed it is constituted by a (1→6)-linked α-d-Galp main-chain, partially methylated at O-3, and partially substituted at O-2 by non-reducing end-units of α-l-Fucp or α-d-Galp.
27112883	5	52	theme	sulfated	776:783	arg1	derivatives					785:795	all sulfated derivatives	772:795	all sulfated derivatives	772:795	The degree of substitution (DS) was evaluated for all sulfated derivatives.
27112883	0	53	theme	characterization	47:62	arg1	Optimization					0:11	Optimization	0:11	Optimization of chemical sulfation, structural characterization and anticoagulant activity of Agaricus bisporus fucogalactan	0:123	Optimization of chemical sulfation, structural characterization and anticoagulant activity of Agaricus bisporus fucogalactan.
27112883	2	54	link	-linked	298:304	arg1	main-chain					315:324	a (1→6)-linked α-d-Galp main-chain	291:324	a (1→6)-linked α-d-Galp main-chain	291:324	The monosaccharide composition, methylation, and NMR analyses showed it is constituted by a (1→6)-linked α-d-Galp main-chain, partially methylated at O-3, and partially substituted at O-2 by non-reducing end-units of α-l-Fucp or α-d-Galp.
27112883	2	55	theme	composition	220:230	arg1	analyses					254:261	The monosaccharide composition, methylation, and NMR analyses	201:261	The monosaccharide composition, methylation, and NMR analyses	201:261	The monosaccharide composition, methylation, and NMR analyses showed it is constituted by a (1→6)-linked α-d-Galp main-chain, partially methylated at O-3, and partially substituted at O-2 by non-reducing end-units of α-l-Fucp or α-d-Galp.
27112883	8	56	theme	15-45μgmL	1207:1215	arg1	concentrations					1189:1202	concentrations	1189:1202	concentrations of 15-45μgmL(-1)	1189:1219	The results showed that E100 produces a linear increment of APTT for concentrations of 15-45μgmL(-1), whereas PT was almost constant between 20 and 400μgmL(-1), suggesting an anticoagulant activity via inhibition of the intrinsic pathway of blood coagulation.
27112883	3	57	dep	showed	455:460	arg1	had					465:467	had	465:467	showed it had Mw of 1.28×10(4)gmol(-1)	455:492	HPSEC analysis showed it had Mw of 1.28×10(4)gmol(-1).
27112883	2	58	theme	monosaccharide	205:218	arg1	composition					220:230	monosaccharide composition	205:230	monosaccharide composition	205:230	The monosaccharide composition, methylation, and NMR analyses showed it is constituted by a (1→6)-linked α-d-Galp main-chain, partially methylated at O-3, and partially substituted at O-2 by non-reducing end-units of α-l-Fucp or α-d-Galp.
27112883	9	59	theme	methylation	1388:1398	arg1	analyses					1400:1407	methylation analyses	1388:1407	methylation analyses	1388:1407	NMR and methylation analyses showed that α-d-Galp units of the main chain were greatly sulfated on 2-O-, 3-O-, and 4-O-positions.
27112883	8	60	dep	showed	1132:1137	arg1	whereas					1222:1228	whereas	1222:1228	whereas	1222:1228	The results showed that E100 produces a linear increment of APTT for concentrations of 15-45μgmL(-1), whereas PT was almost constant between 20 and 400μgmL(-1), suggesting an anticoagulant activity via inhibition of the intrinsic pathway of blood coagulation.
27112883	4	61	theme	molar	552:556	arg1	ratio					558:562	molar ratio	552:562	molar ratio of sulfation agent to hydroxyl group on the polysaccharide (ηClSO3H/OH ratio)	552:640	The polysaccharide was sulfated modifying reaction time, molar ratio of sulfation agent to hydroxyl group on the polysaccharide (ηClSO3H/OH ratio), and ratio of total reaction volume to weight of sample (VT/w ratio; μLmg(-1)).
27112883	4	62	theme	VT/w	699:702	arg1	ratio					704:708	VT/w ratio	699:708	VT/w ratio; μLmg	699:714	The polysaccharide was sulfated modifying reaction time, molar ratio of sulfation agent to hydroxyl group on the polysaccharide (ηClSO3H/OH ratio), and ratio of total reaction volume to weight of sample (VT/w ratio; μLmg(-1)).
27112883	2	63	theme	α-d-Galp	306:313	arg1	main-chain					315:324	a (1→6)-linked α-d-Galp main-chain	291:324	a (1→6)-linked α-d-Galp main-chain	291:324	The monosaccharide composition, methylation, and NMR analyses showed it is constituted by a (1→6)-linked α-d-Galp main-chain, partially methylated at O-3, and partially substituted at O-2 by non-reducing end-units of α-l-Fucp or α-d-Galp.
27112883	8	64	theme	coagulation	1367:1377	arg1	pathway					1350:1356	the intrinsic pathway	1336:1356	the intrinsic pathway of blood coagulation	1336:1377	The results showed that E100 produces a linear increment of APTT for concentrations of 15-45μgmL(-1), whereas PT was almost constant between 20 and 400μgmL(-1), suggesting an anticoagulant activity via inhibition of the intrinsic pathway of blood coagulation.
27112883	0	65	theme	activity	82:89	arg1	Optimization					0:11	Optimization	0:11	Optimization of chemical sulfation, structural characterization and anticoagulant activity of Agaricus bisporus fucogalactan	0:123	Optimization of chemical sulfation, structural characterization and anticoagulant activity of Agaricus bisporus fucogalactan.
27112883	6	66	theme	Activated	896:904	arg1	APTT					935:938	APTT	935:938	APTT	935:938	The sulfated fucogalactan with the highest DS value (2.83) had the best anticoagulant activity on Activated Partial Thromboplastin Time (APTT) and Protrombin Time (PT) assays.
27112883	6	66	theme	Activated	896:904	arg1	Time					929:932	Activated Partial Thromboplastin Time	896:932	Activated Partial Thromboplastin Time (APTT)	896:939	The sulfated fucogalactan with the highest DS value (2.83) had the best anticoagulant activity on Activated Partial Thromboplastin Time (APTT) and Protrombin Time (PT) assays.
27112883	7	67	theme	reaction	1110:1117	arg1	6h					1104:1105	6h	1104:1105	6h of reaction	1104:1117	This sulfated fucogalactan, named E100, was obtained with the optimal conditions of ηClSO3H/OH ratio of 18, VT/w ratio of 100, in 6h of reaction.
27112883	4	68	theme	ηClSO3H/OH	624:633	arg1	ratio					635:639	ηClSO3H/OH ratio	624:639	ηClSO3H/OH ratio	624:639	The polysaccharide was sulfated modifying reaction time, molar ratio of sulfation agent to hydroxyl group on the polysaccharide (ηClSO3H/OH ratio), and ratio of total reaction volume to weight of sample (VT/w ratio; μLmg(-1)).
27112883	4	68	theme	ηClSO3H/OH	624:633	arg1	polysaccharide					608:621	the polysaccharide	604:621	the polysaccharide (ηClSO3H/OH ratio)	604:640	The polysaccharide was sulfated modifying reaction time, molar ratio of sulfation agent to hydroxyl group on the polysaccharide (ηClSO3H/OH ratio), and ratio of total reaction volume to weight of sample (VT/w ratio; μLmg(-1)).
27112883	6	69	theme	anticoagulant	870:882	arg1	activity					884:891	the best anticoagulant activity	861:891	the best anticoagulant activity	861:891	The sulfated fucogalactan with the highest DS value (2.83) had the best anticoagulant activity on Activated Partial Thromboplastin Time (APTT) and Protrombin Time (PT) assays.
27112883	0	70	theme	anticoagulant	68:80	arg1	activity					82:89	anticoagulant activity	68:89	anticoagulant activity	68:89	Optimization of chemical sulfation, structural characterization and anticoagulant activity of Agaricus bisporus fucogalactan.
27112883	2	71	theme	NMR	250:252	arg1	analyses					254:261	The monosaccharide composition, methylation, and NMR analyses	201:261	The monosaccharide composition, methylation, and NMR analyses	201:261	The monosaccharide composition, methylation, and NMR analyses showed it is constituted by a (1→6)-linked α-d-Galp main-chain, partially methylated at O-3, and partially substituted at O-2 by non-reducing end-units of α-l-Fucp or α-d-Galp.
27112883	7	72	theme	ratio	1087:1091	arg1	ratio					1069:1073	ηClSO3H/OH ratio	1058:1073	ηClSO3H/OH ratio of 18, VT/w ratio of 100	1058:1098	This sulfated fucogalactan, named E100, was obtained with the optimal conditions of ηClSO3H/OH ratio of 18, VT/w ratio of 100, in 6h of reaction.
27112883	6	73	theme	best	865:868	arg1	activity					884:891	the best anticoagulant activity	861:891	the best anticoagulant activity	861:891	The sulfated fucogalactan with the highest DS value (2.83) had the best anticoagulant activity on Activated Partial Thromboplastin Time (APTT) and Protrombin Time (PT) assays.
28292682	0	0	theme	non-substituted	79:93	arg1	xylo-oligosaccharides					110:130	non-substituted and acetylated xylo-oligosaccharides	79:130	non-substituted and acetylated xylo-oligosaccharides	79:130	Development and validation of a HILIC-ELSD method for simultaneous analysis of non-substituted and acetylated xylo-oligosaccharides.
28292682	3	1	theme	temperature	456:466	arg1	influences					409:418	The influences	405:418	The influences of mobile phase composition, column temperature and flow rate on the retention time and resolution of XOS	405:524	The influences of mobile phase composition, column temperature and flow rate on the retention time and resolution of XOS were investigated.
28292682	5	2	theme	XOS	817:819	arg1	information					780:790	the structural information	765:790	the structural information of X7, X8 and acetylated XOS	765:819	In addition, LC-ESI-MS was employed to determine the structural information of X7, X8 and acetylated XOS.
28292682	5	3	theme	X8	799:800	arg1	information					780:790	the structural information	765:790	the structural information of X7, X8 and acetylated XOS	765:819	In addition, LC-ESI-MS was employed to determine the structural information of X7, X8 and acetylated XOS.
28292682	1	4	theme	compositional	175:187	arg1	analysis					189:196	compositional analysis	175:196	compositional analysis of both xylo-oligosaccharides (XOS) with degree of polymerization (DP) from 2 to 8 and acetylated XOS with DP from 3 to 8	175:318	A new HILIC-ELSD method was developed for compositional analysis of both xylo-oligosaccharides (XOS) with degree of polymerization (DP) from 2 to 8 and acetylated XOS with DP from 3 to 8.
28292682	0	5	theme	acetylated	99:108	arg1	xylo-oligosaccharides					110:130	non-substituted and acetylated xylo-oligosaccharides	79:130	non-substituted and acetylated xylo-oligosaccharides	79:130	Development and validation of a HILIC-ELSD method for simultaneous analysis of non-substituted and acetylated xylo-oligosaccharides.
28292682	1	6	dep	8	279:279	arg1	to					276:277	to	276:277	to	276:277	A new HILIC-ELSD method was developed for compositional analysis of both xylo-oligosaccharides (XOS) with degree of polymerization (DP) from 2 to 8 and acetylated XOS with DP from 3 to 8.
28292682	3	7	theme	retention	489:497	arg1	time					499:502	retention time	489:502	retention time	489:502	The influences of mobile phase composition, column temperature and flow rate on the retention time and resolution of XOS were investigated.
28292682	6	8	theme	derivatization	871:884	arg1	procedure					886:894	no derivatization procedure	868:894	no derivatization procedure	868:894	The proposed method was simple, reliable, and no derivatization procedure was needed.
28292682	4	9	theme	1mL/min	672:678	arg1	rate					664:667	a flow rate	657:667	a flow rate of 1mL/min	657:678	An excellent separation result was achieved with a linear gradient elution of 75%-50% acetonitrile in 30min, at a flow rate of 1mL/min and the column temperature at 35°C.
28292682	4	9	theme	1mL/min	672:678	arg1	temperature					695:705	the column temperature	684:705	the column temperature at 35°C	684:713	An excellent separation result was achieved with a linear gradient elution of 75%-50% acetonitrile in 30min, at a flow rate of 1mL/min and the column temperature at 35°C.
28292682	1	10	with	XOS	296:298	arg1	degree					239:244	degree	239:244	degree of polymerization (DP)	239:267	A new HILIC-ELSD method was developed for compositional analysis of both xylo-oligosaccharides (XOS) with degree of polymerization (DP) from 2 to 8 and acetylated XOS with DP from 3 to 8.
28292682	1	10	with	XOS	296:298	arg1	DP					305:306	DP	305:306	DP from 3 to 8	305:318	A new HILIC-ELSD method was developed for compositional analysis of both xylo-oligosaccharides (XOS) with degree of polymerization (DP) from 2 to 8 and acetylated XOS with DP from 3 to 8.
28292682	4	11	theme	acetonitrile	631:642	arg1	elution					612:618	a linear gradient elution	594:618	a linear gradient elution of 75%-50% acetonitrile in 30min	594:651	An excellent separation result was achieved with a linear gradient elution of 75%-50% acetonitrile in 30min, at a flow rate of 1mL/min and the column temperature at 35°C.
28292682	4	12	theme	%	629:629	arg1	acetonitrile					631:642	75%-50% acetonitrile	623:642	75%-50% acetonitrile in 30min	623:651	An excellent separation result was achieved with a linear gradient elution of 75%-50% acetonitrile in 30min, at a flow rate of 1mL/min and the column temperature at 35°C.
28292682	4	13	theme	column	688:693	arg1	temperature					695:705	the column temperature	684:705	the column temperature at 35°C	684:713	An excellent separation result was achieved with a linear gradient elution of 75%-50% acetonitrile in 30min, at a flow rate of 1mL/min and the column temperature at 35°C.
28292682	2	14	theme	zwitterionic	353:364	arg1	column					372:377	a zwitterionic HILIC column	351:377	a zwitterionic HILIC column using ELSD as a detector	351:402	The method was carried out on a zwitterionic HILIC column using ELSD as a detector.
28292682	0	15	theme	xylo-oligosaccharides	110:130	arg1	analysis					67:74	simultaneous analysis	54:74	simultaneous analysis of non-substituted and acetylated xylo-oligosaccharides	54:130	Development and validation of a HILIC-ELSD method for simultaneous analysis of non-substituted and acetylated xylo-oligosaccharides.
28292682	0	16	theme	HILIC-ELSD	32:41	arg1	method					43:48	a HILIC-ELSD method	30:48	a HILIC-ELSD method for simultaneous analysis of non-substituted and acetylated xylo-oligosaccharides	30:130	Development and validation of a HILIC-ELSD method for simultaneous analysis of non-substituted and acetylated xylo-oligosaccharides.
28292682	4	17	theme	excellent	548:556	arg1	result					569:574	An excellent separation result	545:574	An excellent separation result	545:574	An excellent separation result was achieved with a linear gradient elution of 75%-50% acetonitrile in 30min, at a flow rate of 1mL/min and the column temperature at 35°C.
28292682	4	18	from	elution	612:618	arg1	30min					647:651	30min	647:651	30min	647:651	An excellent separation result was achieved with a linear gradient elution of 75%-50% acetonitrile in 30min, at a flow rate of 1mL/min and the column temperature at 35°C.
28292682	4	19	theme	gradient	603:610	arg1	elution					612:618	a linear gradient elution	594:618	a linear gradient elution of 75%-50% acetonitrile in 30min	594:651	An excellent separation result was achieved with a linear gradient elution of 75%-50% acetonitrile in 30min, at a flow rate of 1mL/min and the column temperature at 35°C.
28292682	7	20	theme	XOS	973:975	arg1	analysis					941:948	compositional analysis	927:948	compositional analysis	927:948	It is suitable for compositional analysis and quality control of XOS.
28292682	7	20	theme	XOS	973:975	arg1	control					962:968	quality control	954:968	quality control	954:968	It is suitable for compositional analysis and quality control of XOS.
28292682	4	21	theme	flow	659:662	arg1	rate					664:667	a flow rate	657:667	a flow rate of 1mL/min	657:678	An excellent separation result was achieved with a linear gradient elution of 75%-50% acetonitrile in 30min, at a flow rate of 1mL/min and the column temperature at 35°C.
28292682	1	22	dep	8	318:318	arg1	to					315:316	to	315:316	to	315:316	A new HILIC-ELSD method was developed for compositional analysis of both xylo-oligosaccharides (XOS) with degree of polymerization (DP) from 2 to 8 and acetylated XOS with DP from 3 to 8.
28292682	1	23	from	8	279:279	arg1	XOS					229:231	XOS	229:231	XOS	229:231	A new HILIC-ELSD method was developed for compositional analysis of both xylo-oligosaccharides (XOS) with degree of polymerization (DP) from 2 to 8 and acetylated XOS with DP from 3 to 8.
28292682	1	23	from	8	279:279	arg1	analysis					189:196	compositional analysis	175:196	compositional analysis of both xylo-oligosaccharides (XOS) with degree of polymerization (DP) from 2 to 8 and acetylated XOS with DP from 3 to 8	175:318	A new HILIC-ELSD method was developed for compositional analysis of both xylo-oligosaccharides (XOS) with degree of polymerization (DP) from 2 to 8 and acetylated XOS with DP from 3 to 8.
28292682	1	23	from	8	279:279	arg1	xylo-oligosaccharides					206:226	xylo-oligosaccharides	206:226	xylo-oligosaccharides (XOS) with degree of polymerization (DP) from 2 to 8	206:279	A new HILIC-ELSD method was developed for compositional analysis of both xylo-oligosaccharides (XOS) with degree of polymerization (DP) from 2 to 8 and acetylated XOS with DP from 3 to 8.
28292682	1	23	from	8	279:279	arg1	XOS					296:298	acetylated XOS	285:298	acetylated XOS with DP from 3 to 8	285:318	A new HILIC-ELSD method was developed for compositional analysis of both xylo-oligosaccharides (XOS) with degree of polymerization (DP) from 2 to 8 and acetylated XOS with DP from 3 to 8.
28292682	3	24	theme	XOS	522:524	arg1	resolution					508:517	resolution	508:517	resolution	508:517	The influences of mobile phase composition, column temperature and flow rate on the retention time and resolution of XOS were investigated.
28292682	3	24	theme	XOS	522:524	arg1	time					499:502	retention time	489:502	retention time	489:502	The influences of mobile phase composition, column temperature and flow rate on the retention time and resolution of XOS were investigated.
28292682	1	25	theme	xylo-oligosaccharides	206:226	arg1	analysis					189:196	compositional analysis	175:196	compositional analysis of both xylo-oligosaccharides (XOS) with degree of polymerization (DP) from 2 to 8 and acetylated XOS with DP from 3 to 8	175:318	A new HILIC-ELSD method was developed for compositional analysis of both xylo-oligosaccharides (XOS) with degree of polymerization (DP) from 2 to 8 and acetylated XOS with DP from 3 to 8.
28292682	3	26	theme	column	449:454	arg1	temperature					456:466	column temperature	449:466	column temperature	449:466	The influences of mobile phase composition, column temperature and flow rate on the retention time and resolution of XOS were investigated.
28292682	3	27	theme	mobile	423:428	arg1	phase					430:434	mobile phase composition, column temperature and flow rate	423:480	phase	430:434	The influences of mobile phase composition, column temperature and flow rate on the retention time and resolution of XOS were investigated.
28292682	1	28	from	8	318:318	arg1	DP					305:306	DP	305:306	DP from 3 to 8	305:318	A new HILIC-ELSD method was developed for compositional analysis of both xylo-oligosaccharides (XOS) with degree of polymerization (DP) from 2 to 8 and acetylated XOS with DP from 3 to 8.
28292682	1	29	theme	acetylated	285:294	arg1	XOS					296:298	acetylated XOS	285:298	acetylated XOS with DP from 3 to 8	285:318	A new HILIC-ELSD method was developed for compositional analysis of both xylo-oligosaccharides (XOS) with degree of polymerization (DP) from 2 to 8 and acetylated XOS with DP from 3 to 8.
28292682	4	30	theme	linear	596:601	arg1	gradient					603:610	a linear gradient	594:610	a linear gradient elution of 75%-50% acetonitrile in 30min	594:651	An excellent separation result was achieved with a linear gradient elution of 75%-50% acetonitrile in 30min, at a flow rate of 1mL/min and the column temperature at 35°C.
28292682	1	31	theme	XOS	296:298	arg1	analysis					189:196	compositional analysis	175:196	compositional analysis of both xylo-oligosaccharides (XOS) with degree of polymerization (DP) from 2 to 8 and acetylated XOS with DP from 3 to 8	175:318	A new HILIC-ELSD method was developed for compositional analysis of both xylo-oligosaccharides (XOS) with degree of polymerization (DP) from 2 to 8 and acetylated XOS with DP from 3 to 8.
28292682	7	32	theme	quality	954:960	arg1	control					962:968	quality control	954:968	quality control	954:968	It is suitable for compositional analysis and quality control of XOS.
28292682	6	33	dep	simple	846:851	arg1	reliable					854:861	reliable	854:861	reliable	854:861	The proposed method was simple, reliable, and no derivatization procedure was needed.
28292682	6	33	dep	simple	846:851	arg1	simple					846:851	simple	846:851	simple	846:851	The proposed method was simple, reliable, and no derivatization procedure was needed.
28292682	6	33	dep	simple	846:851	arg1	method					835:840	The proposed method	822:840	The proposed method	822:840	The proposed method was simple, reliable, and no derivatization procedure was needed.
28292682	2	34	theme	HILIC	366:370	arg1	column					372:377	a zwitterionic HILIC column	351:377	a zwitterionic HILIC column using ELSD as a detector	351:402	The method was carried out on a zwitterionic HILIC column using ELSD as a detector.
28292682	5	35	theme	X7	795:796	arg1	information					780:790	the structural information	765:790	the structural information of X7, X8 and acetylated XOS	765:819	In addition, LC-ESI-MS was employed to determine the structural information of X7, X8 and acetylated XOS.
28292682	7	36	theme	compositional	927:939	arg1	analysis					941:948	compositional analysis	927:948	compositional analysis	927:948	It is suitable for compositional analysis and quality control of XOS.
28292682	3	37	from	influences	409:418	arg1	resolution					508:517	resolution	508:517	resolution	508:517	The influences of mobile phase composition, column temperature and flow rate on the retention time and resolution of XOS were investigated.
28292682	3	37	from	influences	409:418	arg1	time					499:502	retention time	489:502	retention time	489:502	The influences of mobile phase composition, column temperature and flow rate on the retention time and resolution of XOS were investigated.
28292682	5	38	theme	acetylated	806:815	arg1	XOS					817:819	acetylated XOS	806:819	acetylated XOS	806:819	In addition, LC-ESI-MS was employed to determine the structural information of X7, X8 and acetylated XOS.
28292682	0	39	theme	method	43:48	arg1	validation					16:25	validation	16:25	validation	16:25	Development and validation of a HILIC-ELSD method for simultaneous analysis of non-substituted and acetylated xylo-oligosaccharides.
28292682	0	39	theme	method	43:48	arg1	Development					0:10	Development	0:10	Development	0:10	Development and validation of a HILIC-ELSD method for simultaneous analysis of non-substituted and acetylated xylo-oligosaccharides.
28292682	1	40	with	xylo-oligosaccharides	206:226	arg1	degree					239:244	degree	239:244	degree of polymerization (DP)	239:267	A new HILIC-ELSD method was developed for compositional analysis of both xylo-oligosaccharides (XOS) with degree of polymerization (DP) from 2 to 8 and acetylated XOS with DP from 3 to 8.
28292682	1	40	with	xylo-oligosaccharides	206:226	arg1	DP					305:306	DP	305:306	DP from 3 to 8	305:318	A new HILIC-ELSD method was developed for compositional analysis of both xylo-oligosaccharides (XOS) with degree of polymerization (DP) from 2 to 8 and acetylated XOS with DP from 3 to 8.
28292682	1	41	theme	new	135:137	arg1	method					150:155	A new HILIC-ELSD method	133:155	A new HILIC-ELSD method	133:155	A new HILIC-ELSD method was developed for compositional analysis of both xylo-oligosaccharides (XOS) with degree of polymerization (DP) from 2 to 8 and acetylated XOS with DP from 3 to 8.
28292682	6	42	theme	proposed	826:833	arg1	reliable					854:861	reliable	854:861	reliable	854:861	The proposed method was simple, reliable, and no derivatization procedure was needed.
28292682	6	42	theme	proposed	826:833	arg1	simple					846:851	simple	846:851	simple	846:851	The proposed method was simple, reliable, and no derivatization procedure was needed.
28292682	6	42	theme	proposed	826:833	arg1	method					835:840	The proposed method	822:840	The proposed method	822:840	The proposed method was simple, reliable, and no derivatization procedure was needed.
28292682	4	43	theme	%	625:625	arg1	acetonitrile					631:642	75%-50% acetonitrile	623:642	75%-50% acetonitrile in 30min	623:651	An excellent separation result was achieved with a linear gradient elution of 75%-50% acetonitrile in 30min, at a flow rate of 1mL/min and the column temperature at 35°C.
28292682	1	44	theme	HILIC-ELSD	139:148	arg1	method					150:155	A new HILIC-ELSD method	133:155	A new HILIC-ELSD method	133:155	A new HILIC-ELSD method was developed for compositional analysis of both xylo-oligosaccharides (XOS) with degree of polymerization (DP) from 2 to 8 and acetylated XOS with DP from 3 to 8.
28292682	4	45	theme	separation	558:567	arg1	result					569:574	An excellent separation result	545:574	An excellent separation result	545:574	An excellent separation result was achieved with a linear gradient elution of 75%-50% acetonitrile in 30min, at a flow rate of 1mL/min and the column temperature at 35°C.
28292682	3	46	dep	phase	430:434	arg1	composition					436:446	composition	436:446	composition	436:446	The influences of mobile phase composition, column temperature and flow rate on the retention time and resolution of XOS were investigated.
28292682	3	47	dep	time	499:502	arg1	the					485:487	the	485:487	the	485:487	The influences of mobile phase composition, column temperature and flow rate on the retention time and resolution of XOS were investigated.
28292682	4	48	from	30min	647:651	arg1	elution					612:618	a linear gradient elution	594:618	a linear gradient elution of 75%-50% acetonitrile in 30min	594:651	An excellent separation result was achieved with a linear gradient elution of 75%-50% acetonitrile in 30min, at a flow rate of 1mL/min and the column temperature at 35°C.
28292682	3	49	theme	phase	430:434	arg1	influences					409:418	The influences	405:418	The influences of mobile phase composition, column temperature and flow rate on the retention time and resolution of XOS	405:524	The influences of mobile phase composition, column temperature and flow rate on the retention time and resolution of XOS were investigated.
28292682	4	50	from	35°C	710:713	arg1	rate					664:667	a flow rate	657:667	a flow rate of 1mL/min	657:678	An excellent separation result was achieved with a linear gradient elution of 75%-50% acetonitrile in 30min, at a flow rate of 1mL/min and the column temperature at 35°C.
28292682	4	50	from	35°C	710:713	arg1	temperature					695:705	the column temperature	684:705	the column temperature at 35°C	684:713	An excellent separation result was achieved with a linear gradient elution of 75%-50% acetonitrile in 30min, at a flow rate of 1mL/min and the column temperature at 35°C.
28292682	3	51	theme	flow	472:475	arg1	rate					477:480	flow rate	472:480	flow rate	472:480	The influences of mobile phase composition, column temperature and flow rate on the retention time and resolution of XOS were investigated.
28292682	5	52	theme	structural	769:778	arg1	information					780:790	the structural information	765:790	the structural information of X7, X8 and acetylated XOS	765:819	In addition, LC-ESI-MS was employed to determine the structural information of X7, X8 and acetylated XOS.
28292682	1	53	theme	polymerization	249:262	arg1	degree					239:244	degree	239:244	degree of polymerization (DP)	239:267	A new HILIC-ELSD method was developed for compositional analysis of both xylo-oligosaccharides (XOS) with degree of polymerization (DP) from 2 to 8 and acetylated XOS with DP from 3 to 8.
28292682	0	54	theme	simultaneous	54:65	arg1	analysis					67:74	simultaneous analysis	54:74	simultaneous analysis of non-substituted and acetylated xylo-oligosaccharides	54:130	Development and validation of a HILIC-ELSD method for simultaneous analysis of non-substituted and acetylated xylo-oligosaccharides.
28292682	1	55	from	analysis	189:196	arg1	8					279:279	8	279:279	8	279:279	A new HILIC-ELSD method was developed for compositional analysis of both xylo-oligosaccharides (XOS) with degree of polymerization (DP) from 2 to 8 and acetylated XOS with DP from 3 to 8.
28292682	3	56	theme	rate	477:480	arg1	influences					409:418	The influences	405:418	The influences of mobile phase composition, column temperature and flow rate on the retention time and resolution of XOS	405:524	The influences of mobile phase composition, column temperature and flow rate on the retention time and resolution of XOS were investigated.
28292682	4	57	from	acetonitrile	631:642	arg1	30min					647:651	30min	647:651	30min	647:651	An excellent separation result was achieved with a linear gradient elution of 75%-50% acetonitrile in 30min, at a flow rate of 1mL/min and the column temperature at 35°C.
29084904	2	0	theme	composition	497:507	arg1	characterization					458:473	molecular-level characterization	442:473	molecular-level characterization of the polysaccharide composition, mobility, hydration, and intermolecular interactions of the inflorescence cell wall using solid-state nuclear magnetic resonance spectroscopy and small-angle neutron scattering	442:685	We measured the spatial gradients for elongation rate, osmotic pressure, cell wall thickness, and wall mechanical compliances and coupled these macroscopic measurements with molecular-level characterization of the polysaccharide composition, mobility, hydration, and intermolecular interactions of the inflorescence cell wall using solid-state nuclear magnetic resonance spectroscopy and small-angle neutron scattering.
29084904	4	1	theme	microfibril	1019:1029	arg1	alignment					1031:1039	cellulose microfibril alignment	1009:1039	cellulose microfibril alignment along the growing stem	1009:1062	Neutron-scattering curves showed only subtle changes in wall structure, including a slight increase in cellulose microfibril alignment along the growing stem.
29084904	2	2	theme	resonance	629:637	arg1	spectroscopy					639:650	solid-state nuclear magnetic resonance spectroscopy	600:650	solid-state nuclear magnetic resonance spectroscopy	600:650	We measured the spatial gradients for elongation rate, osmotic pressure, cell wall thickness, and wall mechanical compliances and coupled these macroscopic measurements with molecular-level characterization of the polysaccharide composition, mobility, hydration, and intermolecular interactions of the inflorescence cell wall using solid-state nuclear magnetic resonance spectroscopy and small-angle neutron scattering.
29084904	5	3	theme	resonance	1107:1115	arg1	spectra					1117:1123	solid-state nuclear magnetic resonance spectra	1078:1123	solid-state nuclear magnetic resonance spectra	1078:1123	In contrast, solid-state nuclear magnetic resonance spectra showed substantial decreases in pectin amount, esterification, branching, hydration, and mobility in an apical-to-basal pattern, while the cellulose content increased modestly.
29084904	7	4	theme	stem	1717:1720	arg1	kinematics					1691:1700	the growth kinematics	1680:1700	the growth kinematics of the growing stem	1680:1720	These pectin structural changes may lessen the ability of the cell wall to undergo stress relaxation and irreversible expansion (e.g. induced by expansins), thus contributing to the growth kinematics of the growing stem.
29084904	1	5	theme	∼4	249:250	arg1	cm					252:253	the apical ∼4 cm	238:253	the apical ∼4 cm of the stem	238:265	At early stages of Arabidopsis (Arabidopsis thaliana) flowering, the inflorescence stem undergoes rapid growth, with elongation occurring predominantly in the apical ∼4 cm of the stem.
29084904	2	6	theme	polysaccharide	482:495	arg1	composition					497:507	the polysaccharide composition	478:507	the polysaccharide composition	478:507	We measured the spatial gradients for elongation rate, osmotic pressure, cell wall thickness, and wall mechanical compliances and coupled these macroscopic measurements with molecular-level characterization of the polysaccharide composition, mobility, hydration, and intermolecular interactions of the inflorescence cell wall using solid-state nuclear magnetic resonance spectroscopy and small-angle neutron scattering.
29084904	6	7	from	gradient	1477:1484	arg1	rate					1496:1499	growth rate	1489:1499	growth rate	1489:1499	These results suggest that pectin structural changes are connected with increases in pectin-cellulose interaction and reductions in wall compliances along the apical-to-basal gradient in growth rate.
29084904	2	8	theme	magnetic	620:627	arg1	resonance					629:637	solid-state nuclear magnetic resonance	600:637	solid-state nuclear magnetic resonance spectroscopy	600:650	We measured the spatial gradients for elongation rate, osmotic pressure, cell wall thickness, and wall mechanical compliances and coupled these macroscopic measurements with molecular-level characterization of the polysaccharide composition, mobility, hydration, and intermolecular interactions of the inflorescence cell wall using solid-state nuclear magnetic resonance spectroscopy and small-angle neutron scattering.
29084904	1	9	theme	inflorescence	152:164	arg1	stem					166:169	the inflorescence stem	148:169	the inflorescence stem	148:169	At early stages of Arabidopsis (Arabidopsis thaliana) flowering, the inflorescence stem undergoes rapid growth, with elongation occurring predominantly in the apical ∼4 cm of the stem.
29084904	3	10	theme	rate	850:853	arg1	plots					834:838	plots	834:838	plots of growth rate versus wall compliances	834:877	Force-extension curves revealed a gradient, from high to low, in the plastic and elastic compliances of cell walls along the elongation zone, but plots of growth rate versus wall compliances were strikingly nonlinear.
29084904	0	11	from	Gradients	0:8	arg1	Polysaccharides					32:46	Polysaccharides	32:46	Polysaccharides	32:46	Gradients in Wall Mechanics and Polysaccharides along Growing Inflorescence Stems.
29084904	0	11	from	Gradients	0:8	arg1	Mechanics					18:26	Wall Mechanics	13:26	Wall Mechanics	13:26	Gradients in Wall Mechanics and Polysaccharides along Growing Inflorescence Stems.
29084904	2	12	theme	nuclear	612:618	arg1	resonance					629:637	solid-state nuclear magnetic resonance	600:637	solid-state nuclear magnetic resonance spectroscopy	600:650	We measured the spatial gradients for elongation rate, osmotic pressure, cell wall thickness, and wall mechanical compliances and coupled these macroscopic measurements with molecular-level characterization of the polysaccharide composition, mobility, hydration, and intermolecular interactions of the inflorescence cell wall using solid-state nuclear magnetic resonance spectroscopy and small-angle neutron scattering.
29084904	7	13	theme	structural	1515:1524	arg1	changes					1526:1532	These pectin structural changes	1502:1532	These pectin structural changes	1502:1532	These pectin structural changes may lessen the ability of the cell wall to undergo stress relaxation and irreversible expansion (e.g. induced by expansins), thus contributing to the growth kinematics of the growing stem.
29084904	6	14	theme	wall	1434:1437	arg1	compliances					1439:1449	wall compliances	1434:1449	wall compliances along the apical-to-basal gradient in growth rate	1434:1499	These results suggest that pectin structural changes are connected with increases in pectin-cellulose interaction and reductions in wall compliances along the apical-to-basal gradient in growth rate.
29084904	7	15	theme	irreversible	1607:1618	arg1	expansion					1620:1628	irreversible expansion	1607:1628	irreversible expansion (e.g. induced by expansins)	1607:1656	These pectin structural changes may lessen the ability of the cell wall to undergo stress relaxation and irreversible expansion (e.g. induced by expansins), thus contributing to the growth kinematics of the growing stem.
29084904	2	16	theme	solid-state	600:610	arg1	resonance					629:637	solid-state nuclear magnetic resonance	600:637	solid-state nuclear magnetic resonance spectroscopy	600:650	We measured the spatial gradients for elongation rate, osmotic pressure, cell wall thickness, and wall mechanical compliances and coupled these macroscopic measurements with molecular-level characterization of the polysaccharide composition, mobility, hydration, and intermolecular interactions of the inflorescence cell wall using solid-state nuclear magnetic resonance spectroscopy and small-angle neutron scattering.
29084904	2	17	theme	hydration	520:528	arg1	characterization					458:473	molecular-level characterization	442:473	molecular-level characterization of the polysaccharide composition, mobility, hydration, and intermolecular interactions of the inflorescence cell wall using solid-state nuclear magnetic resonance spectroscopy and small-angle neutron scattering	442:685	We measured the spatial gradients for elongation rate, osmotic pressure, cell wall thickness, and wall mechanical compliances and coupled these macroscopic measurements with molecular-level characterization of the polysaccharide composition, mobility, hydration, and intermolecular interactions of the inflorescence cell wall using solid-state nuclear magnetic resonance spectroscopy and small-angle neutron scattering.
29084904	3	18	theme	plastic	757:763	arg1	compliances					777:787	the plastic and elastic compliances	753:787	the plastic and elastic compliances of cell walls along the elongation zone	753:827	Force-extension curves revealed a gradient, from high to low, in the plastic and elastic compliances of cell walls along the elongation zone, but plots of growth rate versus wall compliances were strikingly nonlinear.
29084904	2	19	theme	neutron	668:674	arg1	scattering					676:685	small-angle neutron scattering	656:685	small-angle neutron scattering	656:685	We measured the spatial gradients for elongation rate, osmotic pressure, cell wall thickness, and wall mechanical compliances and coupled these macroscopic measurements with molecular-level characterization of the polysaccharide composition, mobility, hydration, and intermolecular interactions of the inflorescence cell wall using solid-state nuclear magnetic resonance spectroscopy and small-angle neutron scattering.
29084904	2	20	theme	wall	366:369	arg1	compliances					382:392	wall mechanical compliances	366:392	wall mechanical compliances	366:392	We measured the spatial gradients for elongation rate, osmotic pressure, cell wall thickness, and wall mechanical compliances and coupled these macroscopic measurements with molecular-level characterization of the polysaccharide composition, mobility, hydration, and intermolecular interactions of the inflorescence cell wall using solid-state nuclear magnetic resonance spectroscopy and small-angle neutron scattering.
29084904	5	21	theme	pectin	1157:1162	arg1	amount					1164:1169	pectin amount	1157:1169	pectin amount	1157:1169	In contrast, solid-state nuclear magnetic resonance spectra showed substantial decreases in pectin amount, esterification, branching, hydration, and mobility in an apical-to-basal pattern, while the cellulose content increased modestly.
29084904	2	22	theme	small-angle	656:666	arg1	scattering					676:685	small-angle neutron scattering	656:685	small-angle neutron scattering	656:685	We measured the spatial gradients for elongation rate, osmotic pressure, cell wall thickness, and wall mechanical compliances and coupled these macroscopic measurements with molecular-level characterization of the polysaccharide composition, mobility, hydration, and intermolecular interactions of the inflorescence cell wall using solid-state nuclear magnetic resonance spectroscopy and small-angle neutron scattering.
29084904	7	23	theme	pectin	1508:1513	arg1	changes					1526:1532	These pectin structural changes	1502:1532	These pectin structural changes	1502:1532	These pectin structural changes may lessen the ability of the cell wall to undergo stress relaxation and irreversible expansion (e.g. induced by expansins), thus contributing to the growth kinematics of the growing stem.
29084904	4	24	theme	slight	990:995	arg1	increase					997:1004	a slight increase	988:1004	a slight increase in cellulose microfibril alignment along the growing stem	988:1062	Neutron-scattering curves showed only subtle changes in wall structure, including a slight increase in cellulose microfibril alignment along the growing stem.
29084904	4	25	theme	cellulose	1009:1017	arg1	alignment					1031:1039	cellulose microfibril alignment	1009:1039	cellulose microfibril alignment along the growing stem	1009:1062	Neutron-scattering curves showed only subtle changes in wall structure, including a slight increase in cellulose microfibril alignment along the growing stem.
29084904	7	26	theme	growing	1709:1715	arg1	stem					1717:1720	the growing stem	1705:1720	the growing stem	1705:1720	These pectin structural changes may lessen the ability of the cell wall to undergo stress relaxation and irreversible expansion (e.g. induced by expansins), thus contributing to the growth kinematics of the growing stem.
29084904	1	27	theme	early	86:90	arg1	stages					92:97	early stages	86:97	early stages of Arabidopsis (Arabidopsis thaliana) flowering	86:145	At early stages of Arabidopsis (Arabidopsis thaliana) flowering, the inflorescence stem undergoes rapid growth, with elongation occurring predominantly in the apical ∼4 cm of the stem.
29084904	6	28	from	reductions	1420:1429	arg1	compliances					1439:1449	wall compliances	1434:1449	wall compliances along the apical-to-basal gradient in growth rate	1434:1499	These results suggest that pectin structural changes are connected with increases in pectin-cellulose interaction and reductions in wall compliances along the apical-to-basal gradient in growth rate.
29084904	6	28	from	reductions	1420:1429	arg1	interaction					1404:1414	pectin-cellulose interaction	1387:1414	pectin-cellulose interaction	1387:1414	These results suggest that pectin structural changes are connected with increases in pectin-cellulose interaction and reductions in wall compliances along the apical-to-basal gradient in growth rate.
29084904	1	29	theme	rapid	181:185	arg1	growth					187:192	rapid growth	181:192	rapid growth	181:192	At early stages of Arabidopsis (Arabidopsis thaliana) flowering, the inflorescence stem undergoes rapid growth, with elongation occurring predominantly in the apical ∼4 cm of the stem.
29084904	6	30	theme	pectin-cellulose	1387:1402	arg1	interaction					1404:1414	pectin-cellulose interaction	1387:1414	pectin-cellulose interaction	1387:1414	These results suggest that pectin structural changes are connected with increases in pectin-cellulose interaction and reductions in wall compliances along the apical-to-basal gradient in growth rate.
29084904	1	31	theme	stem	262:265	arg1	cm					252:253	the apical ∼4 cm	238:253	the apical ∼4 cm of the stem	238:265	At early stages of Arabidopsis (Arabidopsis thaliana) flowering, the inflorescence stem undergoes rapid growth, with elongation occurring predominantly in the apical ∼4 cm of the stem.
29084904	4	32	theme	growing	1051:1057	arg1	stem					1059:1062	the growing stem	1047:1062	the growing stem	1047:1062	Neutron-scattering curves showed only subtle changes in wall structure, including a slight increase in cellulose microfibril alignment along the growing stem.
29084904	6	33	theme	structural	1336:1345	arg1	changes					1347:1353	pectin structural changes	1329:1353	pectin structural changes	1329:1353	These results suggest that pectin structural changes are connected with increases in pectin-cellulose interaction and reductions in wall compliances along the apical-to-basal gradient in growth rate.
29084904	2	34	theme	macroscopic	412:422	arg1	measurements					424:435	these macroscopic measurements	406:435	these macroscopic measurements	406:435	We measured the spatial gradients for elongation rate, osmotic pressure, cell wall thickness, and wall mechanical compliances and coupled these macroscopic measurements with molecular-level characterization of the polysaccharide composition, mobility, hydration, and intermolecular interactions of the inflorescence cell wall using solid-state nuclear magnetic resonance spectroscopy and small-angle neutron scattering.
29084904	3	35	dep	high	737:740	arg1	to					742:743	to	742:743	to	742:743	Force-extension curves revealed a gradient, from high to low, in the plastic and elastic compliances of cell walls along the elongation zone, but plots of growth rate versus wall compliances were strikingly nonlinear.
29084904	3	35	dep	high	737:740	arg1	low					745:747	low	745:747	low	745:747	Force-extension curves revealed a gradient, from high to low, in the plastic and elastic compliances of cell walls along the elongation zone, but plots of growth rate versus wall compliances were strikingly nonlinear.
29084904	5	36	theme	substantial	1132:1142	arg1	decreases					1144:1152	substantial decreases	1132:1152	substantial decreases in pectin amount, esterification, branching, hydration, and mobility	1132:1221	In contrast, solid-state nuclear magnetic resonance spectra showed substantial decreases in pectin amount, esterification, branching, hydration, and mobility in an apical-to-basal pattern, while the cellulose content increased modestly.
29084904	2	37	theme	spatial	284:290	arg1	gradients					292:300	the spatial gradients	280:300	the spatial gradients for elongation rate, osmotic pressure, cell wall thickness, and wall mechanical compliances	280:392	We measured the spatial gradients for elongation rate, osmotic pressure, cell wall thickness, and wall mechanical compliances and coupled these macroscopic measurements with molecular-level characterization of the polysaccharide composition, mobility, hydration, and intermolecular interactions of the inflorescence cell wall using solid-state nuclear magnetic resonance spectroscopy and small-angle neutron scattering.
29084904	0	38	theme	Wall	13:16	arg1	Mechanics					18:26	Wall Mechanics	13:26	Wall Mechanics	13:26	Gradients in Wall Mechanics and Polysaccharides along Growing Inflorescence Stems.
29084904	7	39	theme	wall	1569:1572	arg1	ability					1549:1555	the ability	1545:1555	the ability of the cell wall to undergo stress relaxation and irreversible expansion (e.g. induced by expansins)	1545:1656	These pectin structural changes may lessen the ability of the cell wall to undergo stress relaxation and irreversible expansion (e.g. induced by expansins), thus contributing to the growth kinematics of the growing stem.
29084904	3	40	theme	Force-extension	688:702	arg1	curves					704:709	Force-extension curves	688:709	Force-extension curves	688:709	Force-extension curves revealed a gradient, from high to low, in the plastic and elastic compliances of cell walls along the elongation zone, but plots of growth rate versus wall compliances were strikingly nonlinear.
29084904	1	41	theme	Arabidopsis	102:112	arg1	thaliana					127:134	Arabidopsis thaliana	115:134	Arabidopsis thaliana	115:134	At early stages of Arabidopsis (Arabidopsis thaliana) flowering, the inflorescence stem undergoes rapid growth, with elongation occurring predominantly in the apical ∼4 cm of the stem.
29084904	1	41	theme	Arabidopsis	102:112	arg1	flowering					137:145	Arabidopsis (Arabidopsis thaliana) flowering	102:145	Arabidopsis (Arabidopsis thaliana) flowering	102:145	At early stages of Arabidopsis (Arabidopsis thaliana) flowering, the inflorescence stem undergoes rapid growth, with elongation occurring predominantly in the apical ∼4 cm of the stem.
29084904	1	42	theme	flowering	137:145	arg1	stages					92:97	early stages	86:97	early stages of Arabidopsis (Arabidopsis thaliana) flowering	86:145	At early stages of Arabidopsis (Arabidopsis thaliana) flowering, the inflorescence stem undergoes rapid growth, with elongation occurring predominantly in the apical ∼4 cm of the stem.
29084904	5	43	theme	apical-to-basal	1229:1243	arg1	pattern					1245:1251	an apical-to-basal pattern	1226:1251	an apical-to-basal pattern	1226:1251	In contrast, solid-state nuclear magnetic resonance spectra showed substantial decreases in pectin amount, esterification, branching, hydration, and mobility in an apical-to-basal pattern, while the cellulose content increased modestly.
29084904	3	44	theme	elastic	769:775	arg1	compliances					777:787	the plastic and elastic compliances	753:787	the plastic and elastic compliances of cell walls along the elongation zone	753:827	Force-extension curves revealed a gradient, from high to low, in the plastic and elastic compliances of cell walls along the elongation zone, but plots of growth rate versus wall compliances were strikingly nonlinear.
29084904	2	45	theme	interactions	550:561	arg1	characterization					458:473	molecular-level characterization	442:473	molecular-level characterization of the polysaccharide composition, mobility, hydration, and intermolecular interactions of the inflorescence cell wall using solid-state nuclear magnetic resonance spectroscopy and small-angle neutron scattering	442:685	We measured the spatial gradients for elongation rate, osmotic pressure, cell wall thickness, and wall mechanical compliances and coupled these macroscopic measurements with molecular-level characterization of the polysaccharide composition, mobility, hydration, and intermolecular interactions of the inflorescence cell wall using solid-state nuclear magnetic resonance spectroscopy and small-angle neutron scattering.
29084904	4	46	theme	wall	962:965	arg1	structure					967:975	wall structure	962:975	wall structure	962:975	Neutron-scattering curves showed only subtle changes in wall structure, including a slight increase in cellulose microfibril alignment along the growing stem.
29084904	7	47	dep	expansion	1620:1628	arg1	e.g.					1631:1634	e.g.	1631:1634	e.g. induced by expansins	1631:1655	These pectin structural changes may lessen the ability of the cell wall to undergo stress relaxation and irreversible expansion (e.g. induced by expansins), thus contributing to the growth kinematics of the growing stem.
29084904	6	48	theme	apical-to-basal	1461:1475	arg1	gradient					1477:1484	the apical-to-basal gradient	1457:1484	the apical-to-basal gradient in growth rate	1457:1499	These results suggest that pectin structural changes are connected with increases in pectin-cellulose interaction and reductions in wall compliances along the apical-to-basal gradient in growth rate.
29084904	2	49	theme	intermolecular	535:548	arg1	interactions					550:561	intermolecular interactions	535:561	intermolecular interactions	535:561	We measured the spatial gradients for elongation rate, osmotic pressure, cell wall thickness, and wall mechanical compliances and coupled these macroscopic measurements with molecular-level characterization of the polysaccharide composition, mobility, hydration, and intermolecular interactions of the inflorescence cell wall using solid-state nuclear magnetic resonance spectroscopy and small-angle neutron scattering.
29084904	5	50	from	decreases	1144:1152	arg1	esterification					1172:1185	esterification	1172:1185	esterification	1172:1185	In contrast, solid-state nuclear magnetic resonance spectra showed substantial decreases in pectin amount, esterification, branching, hydration, and mobility in an apical-to-basal pattern, while the cellulose content increased modestly.
29084904	5	50	from	decreases	1144:1152	arg1	amount					1164:1169	pectin amount	1157:1169	pectin amount	1157:1169	In contrast, solid-state nuclear magnetic resonance spectra showed substantial decreases in pectin amount, esterification, branching, hydration, and mobility in an apical-to-basal pattern, while the cellulose content increased modestly.
29084904	5	50	from	decreases	1144:1152	arg1	hydration					1199:1207	hydration	1199:1207	hydration	1199:1207	In contrast, solid-state nuclear magnetic resonance spectra showed substantial decreases in pectin amount, esterification, branching, hydration, and mobility in an apical-to-basal pattern, while the cellulose content increased modestly.
29084904	5	50	from	decreases	1144:1152	arg1	mobility					1214:1221	mobility	1214:1221	mobility	1214:1221	In contrast, solid-state nuclear magnetic resonance spectra showed substantial decreases in pectin amount, esterification, branching, hydration, and mobility in an apical-to-basal pattern, while the cellulose content increased modestly.
29084904	5	50	from	decreases	1144:1152	arg1	branching					1188:1196	branching	1188:1196	branching	1188:1196	In contrast, solid-state nuclear magnetic resonance spectra showed substantial decreases in pectin amount, esterification, branching, hydration, and mobility in an apical-to-basal pattern, while the cellulose content increased modestly.
29084904	2	51	theme	mechanical	371:380	arg1	compliances					382:392	wall mechanical compliances	366:392	wall mechanical compliances	366:392	We measured the spatial gradients for elongation rate, osmotic pressure, cell wall thickness, and wall mechanical compliances and coupled these macroscopic measurements with molecular-level characterization of the polysaccharide composition, mobility, hydration, and intermolecular interactions of the inflorescence cell wall using solid-state nuclear magnetic resonance spectroscopy and small-angle neutron scattering.
29084904	0	52	theme	Growing	54:60	arg1	Stems					76:80	Growing Inflorescence Stems	54:80	Growing Inflorescence Stems	54:80	Gradients in Wall Mechanics and Polysaccharides along Growing Inflorescence Stems.
29084904	6	53	theme	growth	1489:1494	arg1	rate					1496:1499	growth rate	1489:1499	growth rate	1489:1499	These results suggest that pectin structural changes are connected with increases in pectin-cellulose interaction and reductions in wall compliances along the apical-to-basal gradient in growth rate.
29084904	4	54	theme	Neutron-scattering	906:923	arg1	curves					925:930	Neutron-scattering curves	906:930	Neutron-scattering curves	906:930	Neutron-scattering curves showed only subtle changes in wall structure, including a slight increase in cellulose microfibril alignment along the growing stem.
29084904	4	55	from	changes	951:957	arg1	structure					967:975	wall structure	962:975	wall structure	962:975	Neutron-scattering curves showed only subtle changes in wall structure, including a slight increase in cellulose microfibril alignment along the growing stem.
29084904	4	56	theme	subtle	944:949	arg1	increase					997:1004	a slight increase	988:1004	a slight increase in cellulose microfibril alignment along the growing stem	988:1062	Neutron-scattering curves showed only subtle changes in wall structure, including a slight increase in cellulose microfibril alignment along the growing stem.
29084904	4	56	theme	subtle	944:949	arg1	changes					951:957	only subtle changes	939:957	only subtle changes	939:957	Neutron-scattering curves showed only subtle changes in wall structure, including a slight increase in cellulose microfibril alignment along the growing stem.
29084904	2	57	theme	osmotic	323:329	arg1	pressure					331:338	osmotic pressure	323:338	osmotic pressure	323:338	We measured the spatial gradients for elongation rate, osmotic pressure, cell wall thickness, and wall mechanical compliances and coupled these macroscopic measurements with molecular-level characterization of the polysaccharide composition, mobility, hydration, and intermolecular interactions of the inflorescence cell wall using solid-state nuclear magnetic resonance spectroscopy and small-angle neutron scattering.
29084904	2	58	theme	molecular-level	442:456	arg1	characterization					458:473	molecular-level characterization	442:473	molecular-level characterization of the polysaccharide composition, mobility, hydration, and intermolecular interactions of the inflorescence cell wall using solid-state nuclear magnetic resonance spectroscopy and small-angle neutron scattering	442:685	We measured the spatial gradients for elongation rate, osmotic pressure, cell wall thickness, and wall mechanical compliances and coupled these macroscopic measurements with molecular-level characterization of the polysaccharide composition, mobility, hydration, and intermolecular interactions of the inflorescence cell wall using solid-state nuclear magnetic resonance spectroscopy and small-angle neutron scattering.
29084904	3	59	theme	cell	792:795	arg1	walls					797:801	cell walls	792:801	cell walls	792:801	Force-extension curves revealed a gradient, from high to low, in the plastic and elastic compliances of cell walls along the elongation zone, but plots of growth rate versus wall compliances were strikingly nonlinear.
29084904	2	60	theme	wall	589:592	arg1	hydration					520:528	hydration	520:528	hydration	520:528	We measured the spatial gradients for elongation rate, osmotic pressure, cell wall thickness, and wall mechanical compliances and coupled these macroscopic measurements with molecular-level characterization of the polysaccharide composition, mobility, hydration, and intermolecular interactions of the inflorescence cell wall using solid-state nuclear magnetic resonance spectroscopy and small-angle neutron scattering.
29084904	2	60	theme	wall	589:592	arg1	mobility					510:517	mobility	510:517	mobility	510:517	We measured the spatial gradients for elongation rate, osmotic pressure, cell wall thickness, and wall mechanical compliances and coupled these macroscopic measurements with molecular-level characterization of the polysaccharide composition, mobility, hydration, and intermolecular interactions of the inflorescence cell wall using solid-state nuclear magnetic resonance spectroscopy and small-angle neutron scattering.
29084904	2	60	theme	wall	589:592	arg1	composition					497:507	the polysaccharide composition	478:507	the polysaccharide composition	478:507	We measured the spatial gradients for elongation rate, osmotic pressure, cell wall thickness, and wall mechanical compliances and coupled these macroscopic measurements with molecular-level characterization of the polysaccharide composition, mobility, hydration, and intermolecular interactions of the inflorescence cell wall using solid-state nuclear magnetic resonance spectroscopy and small-angle neutron scattering.
29084904	2	60	theme	wall	589:592	arg1	interactions					550:561	intermolecular interactions	535:561	intermolecular interactions	535:561	We measured the spatial gradients for elongation rate, osmotic pressure, cell wall thickness, and wall mechanical compliances and coupled these macroscopic measurements with molecular-level characterization of the polysaccharide composition, mobility, hydration, and intermolecular interactions of the inflorescence cell wall using solid-state nuclear magnetic resonance spectroscopy and small-angle neutron scattering.
29084904	7	61	theme	stress	1585:1590	arg1	relaxation					1592:1601	stress relaxation	1585:1601	stress relaxation	1585:1601	These pectin structural changes may lessen the ability of the cell wall to undergo stress relaxation and irreversible expansion (e.g. induced by expansins), thus contributing to the growth kinematics of the growing stem.
29084904	1	62	theme	Arabidopsis	115:125	arg1	thaliana					127:134	Arabidopsis thaliana	115:134	Arabidopsis thaliana	115:134	At early stages of Arabidopsis (Arabidopsis thaliana) flowering, the inflorescence stem undergoes rapid growth, with elongation occurring predominantly in the apical ∼4 cm of the stem.
29084904	1	62	theme	Arabidopsis	115:125	arg1	flowering					137:145	Arabidopsis (Arabidopsis thaliana) flowering	102:145	Arabidopsis (Arabidopsis thaliana) flowering	102:145	At early stages of Arabidopsis (Arabidopsis thaliana) flowering, the inflorescence stem undergoes rapid growth, with elongation occurring predominantly in the apical ∼4 cm of the stem.
29084904	3	63	theme	compliances	867:877	arg1	plots					834:838	plots	834:838	plots of growth rate versus wall compliances	834:877	Force-extension curves revealed a gradient, from high to low, in the plastic and elastic compliances of cell walls along the elongation zone, but plots of growth rate versus wall compliances were strikingly nonlinear.
29084904	3	64	theme	walls	797:801	arg1	compliances					777:787	the plastic and elastic compliances	753:787	the plastic and elastic compliances of cell walls along the elongation zone	753:827	Force-extension curves revealed a gradient, from high to low, in the plastic and elastic compliances of cell walls along the elongation zone, but plots of growth rate versus wall compliances were strikingly nonlinear.
29084904	2	65	theme	cell	584:587	arg1	wall					589:592	the inflorescence cell wall	566:592	the inflorescence cell wall using solid-state nuclear magnetic resonance spectroscopy and small-angle neutron scattering	566:685	We measured the spatial gradients for elongation rate, osmotic pressure, cell wall thickness, and wall mechanical compliances and coupled these macroscopic measurements with molecular-level characterization of the polysaccharide composition, mobility, hydration, and intermolecular interactions of the inflorescence cell wall using solid-state nuclear magnetic resonance spectroscopy and small-angle neutron scattering.
29084904	5	66	theme	solid-state	1078:1088	arg1	resonance					1107:1115	solid-state nuclear magnetic resonance	1078:1115	solid-state nuclear magnetic resonance spectra	1078:1123	In contrast, solid-state nuclear magnetic resonance spectra showed substantial decreases in pectin amount, esterification, branching, hydration, and mobility in an apical-to-basal pattern, while the cellulose content increased modestly.
29084904	5	67	theme	cellulose	1264:1272	arg1	content					1274:1280	the cellulose content	1260:1280	the cellulose content	1260:1280	In contrast, solid-state nuclear magnetic resonance spectra showed substantial decreases in pectin amount, esterification, branching, hydration, and mobility in an apical-to-basal pattern, while the cellulose content increased modestly.
29084904	2	68	theme	inflorescence	570:582	arg1	wall					589:592	the inflorescence cell wall	566:592	the inflorescence cell wall using solid-state nuclear magnetic resonance spectroscopy and small-angle neutron scattering	566:685	We measured the spatial gradients for elongation rate, osmotic pressure, cell wall thickness, and wall mechanical compliances and coupled these macroscopic measurements with molecular-level characterization of the polysaccharide composition, mobility, hydration, and intermolecular interactions of the inflorescence cell wall using solid-state nuclear magnetic resonance spectroscopy and small-angle neutron scattering.
29084904	5	69	theme	nuclear	1090:1096	arg1	resonance					1107:1115	solid-state nuclear magnetic resonance	1078:1115	solid-state nuclear magnetic resonance spectra	1078:1123	In contrast, solid-state nuclear magnetic resonance spectra showed substantial decreases in pectin amount, esterification, branching, hydration, and mobility in an apical-to-basal pattern, while the cellulose content increased modestly.
29084904	3	70	theme	growth	843:848	arg1	rate					850:853	growth rate	843:853	growth rate	843:853	Force-extension curves revealed a gradient, from high to low, in the plastic and elastic compliances of cell walls along the elongation zone, but plots of growth rate versus wall compliances were strikingly nonlinear.
29084904	2	71	theme	elongation	306:315	arg1	rate					317:320	elongation rate	306:320	elongation rate	306:320	We measured the spatial gradients for elongation rate, osmotic pressure, cell wall thickness, and wall mechanical compliances and coupled these macroscopic measurements with molecular-level characterization of the polysaccharide composition, mobility, hydration, and intermolecular interactions of the inflorescence cell wall using solid-state nuclear magnetic resonance spectroscopy and small-angle neutron scattering.
29084904	2	72	theme	mobility	510:517	arg1	characterization					458:473	molecular-level characterization	442:473	molecular-level characterization of the polysaccharide composition, mobility, hydration, and intermolecular interactions of the inflorescence cell wall using solid-state nuclear magnetic resonance spectroscopy and small-angle neutron scattering	442:685	We measured the spatial gradients for elongation rate, osmotic pressure, cell wall thickness, and wall mechanical compliances and coupled these macroscopic measurements with molecular-level characterization of the polysaccharide composition, mobility, hydration, and intermolecular interactions of the inflorescence cell wall using solid-state nuclear magnetic resonance spectroscopy and small-angle neutron scattering.
29084904	5	73	theme	magnetic	1098:1105	arg1	resonance					1107:1115	solid-state nuclear magnetic resonance	1078:1115	solid-state nuclear magnetic resonance spectra	1078:1123	In contrast, solid-state nuclear magnetic resonance spectra showed substantial decreases in pectin amount, esterification, branching, hydration, and mobility in an apical-to-basal pattern, while the cellulose content increased modestly.
29084904	6	74	from	increases	1374:1382	arg1	compliances					1439:1449	wall compliances	1434:1449	wall compliances along the apical-to-basal gradient in growth rate	1434:1499	These results suggest that pectin structural changes are connected with increases in pectin-cellulose interaction and reductions in wall compliances along the apical-to-basal gradient in growth rate.
29084904	6	74	from	increases	1374:1382	arg1	interaction					1404:1414	pectin-cellulose interaction	1387:1414	pectin-cellulose interaction	1387:1414	These results suggest that pectin structural changes are connected with increases in pectin-cellulose interaction and reductions in wall compliances along the apical-to-basal gradient in growth rate.
29084904	7	75	theme	growth	1684:1689	arg1	kinematics					1691:1700	the growth kinematics	1680:1700	the growth kinematics of the growing stem	1680:1720	These pectin structural changes may lessen the ability of the cell wall to undergo stress relaxation and irreversible expansion (e.g. induced by expansins), thus contributing to the growth kinematics of the growing stem.
29084904	2	76	theme	wall	346:349	arg1	thickness					351:359	cell wall thickness	341:359	cell wall thickness	341:359	We measured the spatial gradients for elongation rate, osmotic pressure, cell wall thickness, and wall mechanical compliances and coupled these macroscopic measurements with molecular-level characterization of the polysaccharide composition, mobility, hydration, and intermolecular interactions of the inflorescence cell wall using solid-state nuclear magnetic resonance spectroscopy and small-angle neutron scattering.
29084904	3	77	theme	wall	862:865	arg1	compliances					867:877	wall compliances	862:877	wall compliances	862:877	Force-extension curves revealed a gradient, from high to low, in the plastic and elastic compliances of cell walls along the elongation zone, but plots of growth rate versus wall compliances were strikingly nonlinear.
29084904	3	78	theme	elongation	813:822	arg1	zone					824:827	the elongation zone	809:827	the elongation zone	809:827	Force-extension curves revealed a gradient, from high to low, in the plastic and elastic compliances of cell walls along the elongation zone, but plots of growth rate versus wall compliances were strikingly nonlinear.
29084904	0	79	theme	Inflorescence	62:74	arg1	Stems					76:80	Growing Inflorescence Stems	54:80	Growing Inflorescence Stems	54:80	Gradients in Wall Mechanics and Polysaccharides along Growing Inflorescence Stems.
29084904	7	80	theme	cell	1564:1567	arg1	wall					1569:1572	the cell wall	1560:1572	the cell wall	1560:1572	These pectin structural changes may lessen the ability of the cell wall to undergo stress relaxation and irreversible expansion (e.g. induced by expansins), thus contributing to the growth kinematics of the growing stem.
29084904	4	81	from	increase	997:1004	arg1	alignment					1031:1039	cellulose microfibril alignment	1009:1039	cellulose microfibril alignment along the growing stem	1009:1062	Neutron-scattering curves showed only subtle changes in wall structure, including a slight increase in cellulose microfibril alignment along the growing stem.
29084904	2	82	theme	cell	341:344	arg1	thickness					351:359	cell wall thickness	341:359	cell wall thickness	341:359	We measured the spatial gradients for elongation rate, osmotic pressure, cell wall thickness, and wall mechanical compliances and coupled these macroscopic measurements with molecular-level characterization of the polysaccharide composition, mobility, hydration, and intermolecular interactions of the inflorescence cell wall using solid-state nuclear magnetic resonance spectroscopy and small-angle neutron scattering.
29084904	6	83	theme	pectin	1329:1334	arg1	changes					1347:1353	pectin structural changes	1329:1353	pectin structural changes	1329:1353	These results suggest that pectin structural changes are connected with increases in pectin-cellulose interaction and reductions in wall compliances along the apical-to-basal gradient in growth rate.
29084904	1	84	theme	apical	242:247	arg1	cm					252:253	the apical ∼4 cm	238:253	the apical ∼4 cm of the stem	238:265	At early stages of Arabidopsis (Arabidopsis thaliana) flowering, the inflorescence stem undergoes rapid growth, with elongation occurring predominantly in the apical ∼4 cm of the stem.
29022073	6	0	theme	sites	1280:1284	arg1	reduction					1256:1264	the reduction	1252:1264	the reduction of Al binding sites	1252:1284	Meanwhile, the activity of pectin methylesterase, the level of pectin demethylation, the amounts and the average molecular mass of xyloglucan in the root apices significantly decreased upon exposure to Al, resulting in the reduction of Al binding sites.
29022073	7	1	theme	other	1294:1298	arg1	hand					1300:1303	the other hand	1290:1303	the other hand	1290:1303	On the other hand, the activity and the gene expression of peroxidase decreased, whereas the activity and gene expression of xyloglucan-degrading enzymes, the expression of expansin A and the H +-ATPase4 genes increased in the Al-treated plants.
29022073	4	2	theme	capacity	788:795	arg1	properties					710:719	The mechanical properties	695:719	The mechanical properties of the cell wall	695:736	The mechanical properties of the cell wall, the composition of its polysaccharides and their capacity to absorb Al, the expression of genes, and the activities of the wall-modifying proteins were studied.
29022073	4	2	theme	capacity	788:795	arg1	activities					844:853	the activities	840:853	the activities of the wall-modifying proteins	840:884	The mechanical properties of the cell wall, the composition of its polysaccharides and their capacity to absorb Al, the expression of genes, and the activities of the wall-modifying proteins were studied.
29022073	4	2	theme	capacity	788:795	arg1	composition					743:753	the composition	739:753	the composition of its polysaccharides and their capacity to absorb Al, the expression of genes	739:833	The mechanical properties of the cell wall, the composition of its polysaccharides and their capacity to absorb Al, the expression of genes, and the activities of the wall-modifying proteins were studied.
29022073	6	3	theme	demethylation	1103:1115	arg1	activity					1048:1055	the activity	1044:1055	the activity of pectin methylesterase	1044:1080	Meanwhile, the activity of pectin methylesterase, the level of pectin demethylation, the amounts and the average molecular mass of xyloglucan in the root apices significantly decreased upon exposure to Al, resulting in the reduction of Al binding sites.
29022073	6	3	theme	demethylation	1103:1115	arg1	xyloglucan					1164:1173	xyloglucan	1164:1173	xyloglucan	1164:1173	Meanwhile, the activity of pectin methylesterase, the level of pectin demethylation, the amounts and the average molecular mass of xyloglucan in the root apices significantly decreased upon exposure to Al, resulting in the reduction of Al binding sites.
29022073	6	3	theme	demethylation	1103:1115	arg1	level					1087:1091	the level	1083:1091	the level of pectin demethylation	1083:1115	Meanwhile, the activity of pectin methylesterase, the level of pectin demethylation, the amounts and the average molecular mass of xyloglucan in the root apices significantly decreased upon exposure to Al, resulting in the reduction of Al binding sites.
29022073	6	3	theme	demethylation	1103:1115	arg1	methylesterase					1067:1080	pectin methylesterase	1060:1080	pectin methylesterase	1060:1080	Meanwhile, the activity of pectin methylesterase, the level of pectin demethylation, the amounts and the average molecular mass of xyloglucan in the root apices significantly decreased upon exposure to Al, resulting in the reduction of Al binding sites.
29022073	6	3	theme	demethylation	1103:1115	arg1	demethylation					1103:1115	pectin demethylation	1096:1115	pectin demethylation	1096:1115	Meanwhile, the activity of pectin methylesterase, the level of pectin demethylation, the amounts and the average molecular mass of xyloglucan in the root apices significantly decreased upon exposure to Al, resulting in the reduction of Al binding sites.
29022073	6	3	theme	demethylation	1103:1115	arg1	Meanwhile					1033:1041	Meanwhile	1033:1041	Meanwhile	1033:1041	Meanwhile, the activity of pectin methylesterase, the level of pectin demethylation, the amounts and the average molecular mass of xyloglucan in the root apices significantly decreased upon exposure to Al, resulting in the reduction of Al binding sites.
29022073	6	3	theme	demethylation	1103:1115	arg1	mass					1156:1159	the average molecular mass	1134:1159	the average molecular mass of xyloglucan in the root apices	1134:1192	Meanwhile, the activity of pectin methylesterase, the level of pectin demethylation, the amounts and the average molecular mass of xyloglucan in the root apices significantly decreased upon exposure to Al, resulting in the reduction of Al binding sites.
29022073	6	3	theme	demethylation	1103:1115	arg1	amounts					1122:1128	the amounts	1118:1128	the amounts	1118:1128	Meanwhile, the activity of pectin methylesterase, the level of pectin demethylation, the amounts and the average molecular mass of xyloglucan in the root apices significantly decreased upon exposure to Al, resulting in the reduction of Al binding sites.
29022073	6	4	from	level	1087:1091	arg1	apices					1187:1192	the root apices	1178:1192	the root apices	1178:1192	Meanwhile, the activity of pectin methylesterase, the level of pectin demethylation, the amounts and the average molecular mass of xyloglucan in the root apices significantly decreased upon exposure to Al, resulting in the reduction of Al binding sites.
29022073	1	5	theme	tea	175:177	arg1	seedlings					179:187	tea seedlings	175:187	tea seedlings resulting in biochemical modification of the cell wall	175:242	Treatment with aluminum triggers a unique response in tea seedlings resulting in biochemical modification of the cell wall, regulation of the activity of the loosening agents, and elongation of root.
29022073	7	6	theme	expansin	1460:1467	arg1	A					1469:1469	expansin A	1460:1469	expansin A	1460:1469	On the other hand, the activity and the gene expression of peroxidase decreased, whereas the activity and gene expression of xyloglucan-degrading enzymes, the expression of expansin A and the H +-ATPase4 genes increased in the Al-treated plants.
29022073	9	7	theme	walls	1724:1728	arg1	modification					1699:1710	the biochemical modification	1683:1710	the biochemical modification of the cell walls	1683:1728	From the results, it can be suggested that the biochemical modification of the cell walls reduces sites of Al binding to roots and triggers the activity of the loosening agents, thereby increasing the length of tea roots.
29022073	6	8	theme	xyloglucan	1164:1173	arg1	activity					1048:1055	the activity	1044:1055	the activity of pectin methylesterase	1044:1080	Meanwhile, the activity of pectin methylesterase, the level of pectin demethylation, the amounts and the average molecular mass of xyloglucan in the root apices significantly decreased upon exposure to Al, resulting in the reduction of Al binding sites.
29022073	6	8	theme	xyloglucan	1164:1173	arg1	xyloglucan					1164:1173	xyloglucan	1164:1173	xyloglucan	1164:1173	Meanwhile, the activity of pectin methylesterase, the level of pectin demethylation, the amounts and the average molecular mass of xyloglucan in the root apices significantly decreased upon exposure to Al, resulting in the reduction of Al binding sites.
29022073	6	8	theme	xyloglucan	1164:1173	arg1	level					1087:1091	the level	1083:1091	the level of pectin demethylation	1083:1115	Meanwhile, the activity of pectin methylesterase, the level of pectin demethylation, the amounts and the average molecular mass of xyloglucan in the root apices significantly decreased upon exposure to Al, resulting in the reduction of Al binding sites.
29022073	6	8	theme	xyloglucan	1164:1173	arg1	methylesterase					1067:1080	pectin methylesterase	1060:1080	pectin methylesterase	1060:1080	Meanwhile, the activity of pectin methylesterase, the level of pectin demethylation, the amounts and the average molecular mass of xyloglucan in the root apices significantly decreased upon exposure to Al, resulting in the reduction of Al binding sites.
29022073	6	8	theme	xyloglucan	1164:1173	arg1	demethylation					1103:1115	pectin demethylation	1096:1115	pectin demethylation	1096:1115	Meanwhile, the activity of pectin methylesterase, the level of pectin demethylation, the amounts and the average molecular mass of xyloglucan in the root apices significantly decreased upon exposure to Al, resulting in the reduction of Al binding sites.
29022073	6	8	theme	xyloglucan	1164:1173	arg1	Meanwhile					1033:1041	Meanwhile	1033:1041	Meanwhile	1033:1041	Meanwhile, the activity of pectin methylesterase, the level of pectin demethylation, the amounts and the average molecular mass of xyloglucan in the root apices significantly decreased upon exposure to Al, resulting in the reduction of Al binding sites.
29022073	6	8	theme	xyloglucan	1164:1173	arg1	mass					1156:1159	the average molecular mass	1134:1159	the average molecular mass of xyloglucan in the root apices	1134:1192	Meanwhile, the activity of pectin methylesterase, the level of pectin demethylation, the amounts and the average molecular mass of xyloglucan in the root apices significantly decreased upon exposure to Al, resulting in the reduction of Al binding sites.
29022073	6	8	theme	xyloglucan	1164:1173	arg1	amounts					1122:1128	the amounts	1118:1128	the amounts	1118:1128	Meanwhile, the activity of pectin methylesterase, the level of pectin demethylation, the amounts and the average molecular mass of xyloglucan in the root apices significantly decreased upon exposure to Al, resulting in the reduction of Al binding sites.
29022073	7	9	dep	activity	1380:1387	arg1	the					1376:1378	the	1376:1378	the	1376:1378	On the other hand, the activity and the gene expression of peroxidase decreased, whereas the activity and gene expression of xyloglucan-degrading enzymes, the expression of expansin A and the H +-ATPase4 genes increased in the Al-treated plants.
29022073	4	10	theme	genes	829:833	arg1	expression					815:824	the expression	811:824	the expression of genes	811:833	The mechanical properties of the cell wall, the composition of its polysaccharides and their capacity to absorb Al, the expression of genes, and the activities of the wall-modifying proteins were studied.
29022073	1	11	theme	biochemical	202:212	arg1	modification					214:225	biochemical modification	202:225	biochemical modification of the cell wall	202:242	Treatment with aluminum triggers a unique response in tea seedlings resulting in biochemical modification of the cell wall, regulation of the activity of the loosening agents, and elongation of root.
29022073	6	12	from	mass	1156:1159	arg1	apices					1187:1192	the root apices	1178:1192	the root apices	1178:1192	Meanwhile, the activity of pectin methylesterase, the level of pectin demethylation, the amounts and the average molecular mass of xyloglucan in the root apices significantly decreased upon exposure to Al, resulting in the reduction of Al binding sites.
29022073	6	13	theme	pectin	1060:1065	arg1	methylesterase					1067:1080	pectin methylesterase	1060:1080	pectin methylesterase	1060:1080	Meanwhile, the activity of pectin methylesterase, the level of pectin demethylation, the amounts and the average molecular mass of xyloglucan in the root apices significantly decreased upon exposure to Al, resulting in the reduction of Al binding sites.
29022073	9	14	theme	Al	1747:1748	arg1	Al					1747:1748	Al	1747:1748	Al binding to roots	1747:1765	From the results, it can be suggested that the biochemical modification of the cell walls reduces sites of Al binding to roots and triggers the activity of the loosening agents, thereby increasing the length of tea roots.
29022073	9	14	theme	Al	1747:1748	arg1	sites					1738:1742	sites	1738:1742	sites of Al binding to roots	1738:1765	From the results, it can be suggested that the biochemical modification of the cell walls reduces sites of Al binding to roots and triggers the activity of the loosening agents, thereby increasing the length of tea roots.
29022073	6	15	theme	average	1138:1144	arg1	mass					1156:1159	the average molecular mass	1134:1159	the average molecular mass of xyloglucan in the root apices	1134:1192	Meanwhile, the activity of pectin methylesterase, the level of pectin demethylation, the amounts and the average molecular mass of xyloglucan in the root apices significantly decreased upon exposure to Al, resulting in the reduction of Al binding sites.
29022073	7	16	dep	decreased	1357:1365	arg1	whereas					1368:1374	whereas	1368:1374	whereas	1368:1374	On the other hand, the activity and the gene expression of peroxidase decreased, whereas the activity and gene expression of xyloglucan-degrading enzymes, the expression of expansin A and the H +-ATPase4 genes increased in the Al-treated plants.
29022073	7	17	theme	xyloglucan-degrading	1412:1431	arg1	enzymes					1433:1439	xyloglucan-degrading enzymes	1412:1439	xyloglucan-degrading enzymes	1412:1439	On the other hand, the activity and the gene expression of peroxidase decreased, whereas the activity and gene expression of xyloglucan-degrading enzymes, the expression of expansin A and the H +-ATPase4 genes increased in the Al-treated plants.
29022073	8	18	theme	elastic	1586:1592	arg1	extensibility					1606:1618	elastic and viscous extensibility	1586:1618	elastic and viscous extensibility of the root apices	1586:1637	Interestingly, it was accompanied by the increase of elastic and viscous extensibility of the root apices.
29022073	2	19	theme	elongation	440:449	arg1	promotion					418:426	the promotion	414:426	the promotion of its root elongation	414:449	Unlike most terrestrial plants, tea (Camellia sinensis L.) responds to aluminum (Al) through the promotion of its root elongation; but the real mechanism(s) behind this phenomenon is not well understood.
29022073	5	20	theme	cell	973:976	arg1	wall					978:981	the cell wall	969:981	the cell wall	969:981	With 6 h of the treatment, about 40% of the absorbed Al was bound to the cell wall; however, the amount did not increase thereafter.
29022073	3	21	theme	plausible	527:535	arg1	relationship					537:548	A plausible relationship	525:548	A plausible relationship between the modifications of the cell wall	525:591	A plausible relationship between the modifications of the cell wall and the promotion of root elongation was examined in tea seedlings treated for 8 days with 400 µM Al.
29022073	8	22	theme	viscous	1598:1604	arg1	extensibility					1606:1618	elastic and viscous extensibility	1586:1618	elastic and viscous extensibility of the root apices	1586:1637	Interestingly, it was accompanied by the increase of elastic and viscous extensibility of the root apices.
29022073	4	23	theme	proteins	877:884	arg1	properties					710:719	The mechanical properties	695:719	The mechanical properties of the cell wall	695:736	The mechanical properties of the cell wall, the composition of its polysaccharides and their capacity to absorb Al, the expression of genes, and the activities of the wall-modifying proteins were studied.
29022073	4	23	theme	proteins	877:884	arg1	activities					844:853	the activities	840:853	the activities of the wall-modifying proteins	840:884	The mechanical properties of the cell wall, the composition of its polysaccharides and their capacity to absorb Al, the expression of genes, and the activities of the wall-modifying proteins were studied.
29022073	4	23	theme	proteins	877:884	arg1	composition					743:753	the composition	739:753	the composition of its polysaccharides and their capacity to absorb Al, the expression of genes	739:833	The mechanical properties of the cell wall, the composition of its polysaccharides and their capacity to absorb Al, the expression of genes, and the activities of the wall-modifying proteins were studied.
29022073	6	24	from	Meanwhile	1033:1041	arg1	apices					1187:1192	the root apices	1178:1192	the root apices	1178:1192	Meanwhile, the activity of pectin methylesterase, the level of pectin demethylation, the amounts and the average molecular mass of xyloglucan in the root apices significantly decreased upon exposure to Al, resulting in the reduction of Al binding sites.
29022073	0	25	theme	Camellia	62:69	arg1	seedlings					80:88	Camellia sinensis seedlings	62:88	Camellia sinensis seedlings	62:88	The contribution of cell wall composition in the expansion of Camellia sinensis seedlings roots in response to aluminum.
29022073	1	26	theme	root	315:318	arg1	elongation					301:310	elongation	301:310	elongation of root	301:318	Treatment with aluminum triggers a unique response in tea seedlings resulting in biochemical modification of the cell wall, regulation of the activity of the loosening agents, and elongation of root.
29022073	1	26	theme	root	315:318	arg1	regulation					245:254	regulation	245:254	regulation of the activity of the loosening agents	245:294	Treatment with aluminum triggers a unique response in tea seedlings resulting in biochemical modification of the cell wall, regulation of the activity of the loosening agents, and elongation of root.
29022073	1	26	theme	root	315:318	arg1	response					163:170	a unique response	154:170	a unique response in tea seedlings resulting in biochemical modification of the cell wall	154:242	Treatment with aluminum triggers a unique response in tea seedlings resulting in biochemical modification of the cell wall, regulation of the activity of the loosening agents, and elongation of root.
29022073	2	27	theme	real	460:463	arg1	s					475:475	the real mechanism(s)	456:476	the real mechanism(s) behind this phenomenon	456:499	Unlike most terrestrial plants, tea (Camellia sinensis L.) responds to aluminum (Al) through the promotion of its root elongation; but the real mechanism(s) behind this phenomenon is not well understood.
29022073	0	28	theme	seedlings	80:88	arg1	expansion					49:57	the expansion	45:57	the expansion of Camellia sinensis seedlings	45:88	The contribution of cell wall composition in the expansion of Camellia sinensis seedlings roots in response to aluminum.
29022073	8	29	theme	root	1627:1630	arg1	apices					1632:1637	the root apices	1623:1637	the root apices	1623:1637	Interestingly, it was accompanied by the increase of elastic and viscous extensibility of the root apices.
29022073	9	30	theme	loosening	1800:1808	arg1	agents					1810:1815	the loosening agents	1796:1815	the loosening agents	1796:1815	From the results, it can be suggested that the biochemical modification of the cell walls reduces sites of Al binding to roots and triggers the activity of the loosening agents, thereby increasing the length of tea roots.
29022073	1	31	with	Treatment	121:129	arg1	aluminum					136:143	aluminum	136:143	aluminum	136:143	Treatment with aluminum triggers a unique response in tea seedlings resulting in biochemical modification of the cell wall, regulation of the activity of the loosening agents, and elongation of root.
29022073	7	32	theme	+-ATPase4	1481:1489	arg1	genes					1491:1495	the H +-ATPase4 genes	1475:1495	the H +-ATPase4 genes	1475:1495	On the other hand, the activity and the gene expression of peroxidase decreased, whereas the activity and gene expression of xyloglucan-degrading enzymes, the expression of expansin A and the H +-ATPase4 genes increased in the Al-treated plants.
29022073	7	33	theme	A	1469:1469	arg1	expression					1446:1455	the expression	1442:1455	the expression of expansin A	1442:1469	On the other hand, the activity and the gene expression of peroxidase decreased, whereas the activity and gene expression of xyloglucan-degrading enzymes, the expression of expansin A and the H +-ATPase4 genes increased in the Al-treated plants.
29022073	7	33	theme	A	1469:1469	arg1	genes					1491:1495	the H +-ATPase4 genes	1475:1495	the H +-ATPase4 genes	1475:1495	On the other hand, the activity and the gene expression of peroxidase decreased, whereas the activity and gene expression of xyloglucan-degrading enzymes, the expression of expansin A and the H +-ATPase4 genes increased in the Al-treated plants.
29022073	7	33	theme	A	1469:1469	arg1	activity					1380:1387	activity	1380:1387	activity	1380:1387	On the other hand, the activity and the gene expression of peroxidase decreased, whereas the activity and gene expression of xyloglucan-degrading enzymes, the expression of expansin A and the H +-ATPase4 genes increased in the Al-treated plants.
29022073	7	33	theme	A	1469:1469	arg1	expression					1398:1407	gene expression	1393:1407	gene expression	1393:1407	On the other hand, the activity and the gene expression of peroxidase decreased, whereas the activity and gene expression of xyloglucan-degrading enzymes, the expression of expansin A and the H +-ATPase4 genes increased in the Al-treated plants.
29022073	4	34	theme	cell	728:731	arg1	wall					733:736	the cell wall	724:736	the cell wall	724:736	The mechanical properties of the cell wall, the composition of its polysaccharides and their capacity to absorb Al, the expression of genes, and the activities of the wall-modifying proteins were studied.
29022073	0	35	theme	wall	25:28	arg1	composition					30:40	cell wall composition	20:40	cell wall composition	20:40	The contribution of cell wall composition in the expansion of Camellia sinensis seedlings roots in response to aluminum.
29022073	1	36	theme	loosening	279:287	arg1	agents					289:294	the loosening agents	275:294	the loosening agents	275:294	Treatment with aluminum triggers a unique response in tea seedlings resulting in biochemical modification of the cell wall, regulation of the activity of the loosening agents, and elongation of root.
29022073	7	37	theme	gene	1327:1330	arg1	expression					1332:1341	the gene expression	1323:1341	the gene expression of peroxidase	1323:1355	On the other hand, the activity and the gene expression of peroxidase decreased, whereas the activity and gene expression of xyloglucan-degrading enzymes, the expression of expansin A and the H +-ATPase4 genes increased in the Al-treated plants.
29022073	6	38	dep	sites	1280:1284	arg1	Al					1269:1270	Al binding sites	1269:1284	Al binding sites	1269:1284	Meanwhile, the activity of pectin methylesterase, the level of pectin demethylation, the amounts and the average molecular mass of xyloglucan in the root apices significantly decreased upon exposure to Al, resulting in the reduction of Al binding sites.
29022073	9	39	theme	tea	1851:1853	arg1	roots					1855:1859	tea roots	1851:1859	tea roots	1851:1859	From the results, it can be suggested that the biochemical modification of the cell walls reduces sites of Al binding to roots and triggers the activity of the loosening agents, thereby increasing the length of tea roots.
29022073	2	40	dep	responds	380:387	arg1	Camellia					358:365	Camellia	358:365	Camellia	358:365	Unlike most terrestrial plants, tea (Camellia sinensis L.) responds to aluminum (Al) through the promotion of its root elongation; but the real mechanism(s) behind this phenomenon is not well understood.
29022073	5	41	theme	treatment	916:924	arg1	6 h					905:907	6 h	905:907	6 h of the treatment	905:924	With 6 h of the treatment, about 40% of the absorbed Al was bound to the cell wall; however, the amount did not increase thereafter.
29022073	4	42	theme	mechanical	699:708	arg1	properties					710:719	The mechanical properties	695:719	The mechanical properties of the cell wall	695:736	The mechanical properties of the cell wall, the composition of its polysaccharides and their capacity to absorb Al, the expression of genes, and the activities of the wall-modifying proteins were studied.
29022073	3	43	theme	wall	588:591	arg1	modifications					562:574	the modifications	558:574	the modifications of the cell wall	558:591	A plausible relationship between the modifications of the cell wall and the promotion of root elongation was examined in tea seedlings treated for 8 days with 400 µM Al.
29022073	2	44	dep	Camellia	358:365	arg1	L.					376:377	Camellia sinensis L.	358:377	Camellia sinensis L.	358:377	Unlike most terrestrial plants, tea (Camellia sinensis L.) responds to aluminum (Al) through the promotion of its root elongation; but the real mechanism(s) behind this phenomenon is not well understood.
29022073	6	45	from	amounts	1122:1128	arg1	apices					1187:1192	the root apices	1178:1192	the root apices	1178:1192	Meanwhile, the activity of pectin methylesterase, the level of pectin demethylation, the amounts and the average molecular mass of xyloglucan in the root apices significantly decreased upon exposure to Al, resulting in the reduction of Al binding sites.
29022073	3	46	theme	elongation	619:628	arg1	promotion					601:609	the promotion	597:609	the promotion of root elongation	597:628	A plausible relationship between the modifications of the cell wall and the promotion of root elongation was examined in tea seedlings treated for 8 days with 400 µM Al.
29022073	3	46	theme	elongation	619:628	arg1	relationship					537:548	A plausible relationship	525:548	A plausible relationship between the modifications of the cell wall	525:591	A plausible relationship between the modifications of the cell wall and the promotion of root elongation was examined in tea seedlings treated for 8 days with 400 µM Al.
29022073	1	47	theme	wall	239:242	arg1	modification					214:225	biochemical modification	202:225	biochemical modification of the cell wall	202:242	Treatment with aluminum triggers a unique response in tea seedlings resulting in biochemical modification of the cell wall, regulation of the activity of the loosening agents, and elongation of root.
29022073	4	48	theme	polysaccharides	762:776	arg1	properties					710:719	The mechanical properties	695:719	The mechanical properties of the cell wall	695:736	The mechanical properties of the cell wall, the composition of its polysaccharides and their capacity to absorb Al, the expression of genes, and the activities of the wall-modifying proteins were studied.
29022073	4	48	theme	polysaccharides	762:776	arg1	activities					844:853	the activities	840:853	the activities of the wall-modifying proteins	840:884	The mechanical properties of the cell wall, the composition of its polysaccharides and their capacity to absorb Al, the expression of genes, and the activities of the wall-modifying proteins were studied.
29022073	4	48	theme	polysaccharides	762:776	arg1	composition					743:753	the composition	739:753	the composition of its polysaccharides and their capacity to absorb Al, the expression of genes	739:833	The mechanical properties of the cell wall, the composition of its polysaccharides and their capacity to absorb Al, the expression of genes, and the activities of the wall-modifying proteins were studied.
29022073	6	49	theme	binding	1272:1278	arg1	sites					1280:1284	binding sites	1272:1284	Al binding sites	1269:1284	Meanwhile, the activity of pectin methylesterase, the level of pectin demethylation, the amounts and the average molecular mass of xyloglucan in the root apices significantly decreased upon exposure to Al, resulting in the reduction of Al binding sites.
29022073	9	50	theme	biochemical	1687:1697	arg1	modification					1699:1710	the biochemical modification	1683:1710	the biochemical modification of the cell walls	1683:1728	From the results, it can be suggested that the biochemical modification of the cell walls reduces sites of Al binding to roots and triggers the activity of the loosening agents, thereby increasing the length of tea roots.
29022073	3	51	theme	tea	646:648	arg1	seedlings					650:658	tea seedlings	646:658	tea seedlings treated for 8 days with 400 µM Al	646:692	A plausible relationship between the modifications of the cell wall and the promotion of root elongation was examined in tea seedlings treated for 8 days with 400 µM Al.
29022073	6	52	theme	pectin	1096:1101	arg1	demethylation					1103:1115	pectin demethylation	1096:1115	pectin demethylation	1096:1115	Meanwhile, the activity of pectin methylesterase, the level of pectin demethylation, the amounts and the average molecular mass of xyloglucan in the root apices significantly decreased upon exposure to Al, resulting in the reduction of Al binding sites.
29022073	1	53	theme	unique	156:161	arg1	response					163:170	a unique response	154:170	a unique response in tea seedlings resulting in biochemical modification of the cell wall	154:242	Treatment with aluminum triggers a unique response in tea seedlings resulting in biochemical modification of the cell wall, regulation of the activity of the loosening agents, and elongation of root.
29022073	2	54	dep	s	475:475	arg1	behind					478:483	behind	478:483	behind this phenomenon	478:499	Unlike most terrestrial plants, tea (Camellia sinensis L.) responds to aluminum (Al) through the promotion of its root elongation; but the real mechanism(s) behind this phenomenon is not well understood.
29022073	9	55	theme	cell	1719:1722	arg1	walls					1724:1728	the cell walls	1715:1728	the cell walls	1715:1728	From the results, it can be suggested that the biochemical modification of the cell walls reduces sites of Al binding to roots and triggers the activity of the loosening agents, thereby increasing the length of tea roots.
29022073	1	56	from	regulation	245:254	arg1	seedlings					179:187	tea seedlings	175:187	tea seedlings resulting in biochemical modification of the cell wall	175:242	Treatment with aluminum triggers a unique response in tea seedlings resulting in biochemical modification of the cell wall, regulation of the activity of the loosening agents, and elongation of root.
29022073	7	57	theme	enzymes	1433:1439	arg1	expression					1446:1455	the expression	1442:1455	the expression of expansin A	1442:1469	On the other hand, the activity and the gene expression of peroxidase decreased, whereas the activity and gene expression of xyloglucan-degrading enzymes, the expression of expansin A and the H +-ATPase4 genes increased in the Al-treated plants.
29022073	7	57	theme	enzymes	1433:1439	arg1	genes					1491:1495	the H +-ATPase4 genes	1475:1495	the H +-ATPase4 genes	1475:1495	On the other hand, the activity and the gene expression of peroxidase decreased, whereas the activity and gene expression of xyloglucan-degrading enzymes, the expression of expansin A and the H +-ATPase4 genes increased in the Al-treated plants.
29022073	7	57	theme	enzymes	1433:1439	arg1	activity					1380:1387	activity	1380:1387	activity	1380:1387	On the other hand, the activity and the gene expression of peroxidase decreased, whereas the activity and gene expression of xyloglucan-degrading enzymes, the expression of expansin A and the H +-ATPase4 genes increased in the Al-treated plants.
29022073	7	57	theme	enzymes	1433:1439	arg1	expression					1398:1407	gene expression	1393:1407	gene expression	1393:1407	On the other hand, the activity and the gene expression of peroxidase decreased, whereas the activity and gene expression of xyloglucan-degrading enzymes, the expression of expansin A and the H +-ATPase4 genes increased in the Al-treated plants.
29022073	2	58	theme	mechanism	465:473	arg1	s					475:475	the real mechanism(s)	456:476	the real mechanism(s) behind this phenomenon	456:499	Unlike most terrestrial plants, tea (Camellia sinensis L.) responds to aluminum (Al) through the promotion of its root elongation; but the real mechanism(s) behind this phenomenon is not well understood.
29022073	6	59	theme	methylesterase	1067:1080	arg1	activity					1048:1055	the activity	1044:1055	the activity of pectin methylesterase	1044:1080	Meanwhile, the activity of pectin methylesterase, the level of pectin demethylation, the amounts and the average molecular mass of xyloglucan in the root apices significantly decreased upon exposure to Al, resulting in the reduction of Al binding sites.
29022073	6	59	theme	methylesterase	1067:1080	arg1	xyloglucan					1164:1173	xyloglucan	1164:1173	xyloglucan	1164:1173	Meanwhile, the activity of pectin methylesterase, the level of pectin demethylation, the amounts and the average molecular mass of xyloglucan in the root apices significantly decreased upon exposure to Al, resulting in the reduction of Al binding sites.
29022073	6	59	theme	methylesterase	1067:1080	arg1	level					1087:1091	the level	1083:1091	the level of pectin demethylation	1083:1115	Meanwhile, the activity of pectin methylesterase, the level of pectin demethylation, the amounts and the average molecular mass of xyloglucan in the root apices significantly decreased upon exposure to Al, resulting in the reduction of Al binding sites.
29022073	6	59	theme	methylesterase	1067:1080	arg1	methylesterase					1067:1080	pectin methylesterase	1060:1080	pectin methylesterase	1060:1080	Meanwhile, the activity of pectin methylesterase, the level of pectin demethylation, the amounts and the average molecular mass of xyloglucan in the root apices significantly decreased upon exposure to Al, resulting in the reduction of Al binding sites.
29022073	6	59	theme	methylesterase	1067:1080	arg1	demethylation					1103:1115	pectin demethylation	1096:1115	pectin demethylation	1096:1115	Meanwhile, the activity of pectin methylesterase, the level of pectin demethylation, the amounts and the average molecular mass of xyloglucan in the root apices significantly decreased upon exposure to Al, resulting in the reduction of Al binding sites.
29022073	6	59	theme	methylesterase	1067:1080	arg1	Meanwhile					1033:1041	Meanwhile	1033:1041	Meanwhile	1033:1041	Meanwhile, the activity of pectin methylesterase, the level of pectin demethylation, the amounts and the average molecular mass of xyloglucan in the root apices significantly decreased upon exposure to Al, resulting in the reduction of Al binding sites.
29022073	6	59	theme	methylesterase	1067:1080	arg1	mass					1156:1159	the average molecular mass	1134:1159	the average molecular mass of xyloglucan in the root apices	1134:1192	Meanwhile, the activity of pectin methylesterase, the level of pectin demethylation, the amounts and the average molecular mass of xyloglucan in the root apices significantly decreased upon exposure to Al, resulting in the reduction of Al binding sites.
29022073	6	59	theme	methylesterase	1067:1080	arg1	amounts					1122:1128	the amounts	1118:1128	the amounts	1118:1128	Meanwhile, the activity of pectin methylesterase, the level of pectin demethylation, the amounts and the average molecular mass of xyloglucan in the root apices significantly decreased upon exposure to Al, resulting in the reduction of Al binding sites.
29022073	6	60	theme	molecular	1146:1154	arg1	mass					1156:1159	the average molecular mass	1134:1159	the average molecular mass of xyloglucan in the root apices	1134:1192	Meanwhile, the activity of pectin methylesterase, the level of pectin demethylation, the amounts and the average molecular mass of xyloglucan in the root apices significantly decreased upon exposure to Al, resulting in the reduction of Al binding sites.
29022073	5	61	theme	Al	953:954	arg1	%					935:935	about 40%	927:935	about 40% of the absorbed Al	927:954	With 6 h of the treatment, about 40% of the absorbed Al was bound to the cell wall; however, the amount did not increase thereafter.
29022073	5	61	theme	Al	953:954	arg1	Al					953:954	the absorbed Al	940:954	the absorbed Al	940:954	With 6 h of the treatment, about 40% of the absorbed Al was bound to the cell wall; however, the amount did not increase thereafter.
29022073	7	62	theme	gene	1393:1396	arg1	expression					1398:1407	gene expression	1393:1407	gene expression	1393:1407	On the other hand, the activity and the gene expression of peroxidase decreased, whereas the activity and gene expression of xyloglucan-degrading enzymes, the expression of expansin A and the H +-ATPase4 genes increased in the Al-treated plants.
29022073	0	63	from	roots	90:94	arg1	response					99:106	response	99:106	response to aluminum	99:118	The contribution of cell wall composition in the expansion of Camellia sinensis seedlings roots in response to aluminum.
29022073	2	64	theme	root	435:438	arg1	elongation					440:449	its root elongation	431:449	its root elongation	431:449	Unlike most terrestrial plants, tea (Camellia sinensis L.) responds to aluminum (Al) through the promotion of its root elongation; but the real mechanism(s) behind this phenomenon is not well understood.
29022073	0	65	theme	sinensis	71:78	arg1	seedlings					80:88	Camellia sinensis seedlings	62:88	Camellia sinensis seedlings	62:88	The contribution of cell wall composition in the expansion of Camellia sinensis seedlings roots in response to aluminum.
29022073	1	66	from	response	163:170	arg1	seedlings					179:187	tea seedlings	175:187	tea seedlings resulting in biochemical modification of the cell wall	175:242	Treatment with aluminum triggers a unique response in tea seedlings resulting in biochemical modification of the cell wall, regulation of the activity of the loosening agents, and elongation of root.
29022073	4	67	theme	wall-modifying	862:875	arg1	proteins					877:884	the wall-modifying proteins	858:884	the wall-modifying proteins	858:884	The mechanical properties of the cell wall, the composition of its polysaccharides and their capacity to absorb Al, the expression of genes, and the activities of the wall-modifying proteins were studied.
29022073	0	68	from	contribution	4:15	arg1	expansion					49:57	the expansion	45:57	the expansion of Camellia sinensis seedlings	45:88	The contribution of cell wall composition in the expansion of Camellia sinensis seedlings roots in response to aluminum.
29022073	6	69	from	activity	1048:1055	arg1	apices					1187:1192	the root apices	1178:1192	the root apices	1178:1192	Meanwhile, the activity of pectin methylesterase, the level of pectin demethylation, the amounts and the average molecular mass of xyloglucan in the root apices significantly decreased upon exposure to Al, resulting in the reduction of Al binding sites.
29022073	8	70	theme	extensibility	1606:1618	arg1	increase					1574:1581	the increase	1570:1581	the increase of elastic and viscous extensibility of the root apices	1570:1637	Interestingly, it was accompanied by the increase of elastic and viscous extensibility of the root apices.
29022073	7	71	theme	Al-treated	1514:1523	arg1	plants					1525:1530	the Al-treated plants	1510:1530	the Al-treated plants	1510:1530	On the other hand, the activity and the gene expression of peroxidase decreased, whereas the activity and gene expression of xyloglucan-degrading enzymes, the expression of expansin A and the H +-ATPase4 genes increased in the Al-treated plants.
29022073	4	72	dep	expression	815:824	arg1	Al					807:808	Al	807:808	Al	807:808	The mechanical properties of the cell wall, the composition of its polysaccharides and their capacity to absorb Al, the expression of genes, and the activities of the wall-modifying proteins were studied.
29022073	2	73	dep	aluminum	392:399	arg1	Al					402:403	Al	402:403	Al	402:403	Unlike most terrestrial plants, tea (Camellia sinensis L.) responds to aluminum (Al) through the promotion of its root elongation; but the real mechanism(s) behind this phenomenon is not well understood.
29022073	9	74	theme	agents	1810:1815	arg1	activity					1784:1791	the activity	1780:1791	the activity of the loosening agents	1780:1815	From the results, it can be suggested that the biochemical modification of the cell walls reduces sites of Al binding to roots and triggers the activity of the loosening agents, thereby increasing the length of tea roots.
29022073	6	75	theme	root	1182:1185	arg1	apices					1187:1192	the root apices	1178:1192	the root apices	1178:1192	Meanwhile, the activity of pectin methylesterase, the level of pectin demethylation, the amounts and the average molecular mass of xyloglucan in the root apices significantly decreased upon exposure to Al, resulting in the reduction of Al binding sites.
29022073	8	76	theme	apices	1632:1637	arg1	extensibility					1606:1618	elastic and viscous extensibility	1586:1618	elastic and viscous extensibility of the root apices	1586:1637	Interestingly, it was accompanied by the increase of elastic and viscous extensibility of the root apices.
29022073	0	77	theme	cell	20:23	arg1	composition					30:40	cell wall composition	20:40	cell wall composition	20:40	The contribution of cell wall composition in the expansion of Camellia sinensis seedlings roots in response to aluminum.
29022073	1	78	theme	activity	263:270	arg1	elongation					301:310	elongation	301:310	elongation of root	301:318	Treatment with aluminum triggers a unique response in tea seedlings resulting in biochemical modification of the cell wall, regulation of the activity of the loosening agents, and elongation of root.
29022073	1	78	theme	activity	263:270	arg1	regulation					245:254	regulation	245:254	regulation of the activity of the loosening agents	245:294	Treatment with aluminum triggers a unique response in tea seedlings resulting in biochemical modification of the cell wall, regulation of the activity of the loosening agents, and elongation of root.
29022073	1	78	theme	activity	263:270	arg1	response					163:170	a unique response	154:170	a unique response in tea seedlings resulting in biochemical modification of the cell wall	154:242	Treatment with aluminum triggers a unique response in tea seedlings resulting in biochemical modification of the cell wall, regulation of the activity of the loosening agents, and elongation of root.
29022073	7	79	theme	H	1479:1479	arg1	genes					1491:1495	the H +-ATPase4 genes	1475:1495	the H +-ATPase4 genes	1475:1495	On the other hand, the activity and the gene expression of peroxidase decreased, whereas the activity and gene expression of xyloglucan-degrading enzymes, the expression of expansin A and the H +-ATPase4 genes increased in the Al-treated plants.
29022073	0	80	theme	composition	30:40	arg1	contribution					4:15	The contribution	0:15	The contribution of cell wall composition in the expansion of Camellia sinensis seedlings	0:88	The contribution of cell wall composition in the expansion of Camellia sinensis seedlings roots in response to aluminum.
29022073	7	81	theme	peroxidase	1346:1355	arg1	expression					1332:1341	the gene expression	1323:1341	the gene expression of peroxidase	1323:1355	On the other hand, the activity and the gene expression of peroxidase decreased, whereas the activity and gene expression of xyloglucan-degrading enzymes, the expression of expansin A and the H +-ATPase4 genes increased in the Al-treated plants.
29022073	7	81	theme	peroxidase	1346:1355	arg1	activity					1310:1317	the activity	1306:1317	the activity	1306:1317	On the other hand, the activity and the gene expression of peroxidase decreased, whereas the activity and gene expression of xyloglucan-degrading enzymes, the expression of expansin A and the H +-ATPase4 genes increased in the Al-treated plants.
29022073	1	82	from	elongation	301:310	arg1	seedlings					179:187	tea seedlings	175:187	tea seedlings resulting in biochemical modification of the cell wall	175:242	Treatment with aluminum triggers a unique response in tea seedlings resulting in biochemical modification of the cell wall, regulation of the activity of the loosening agents, and elongation of root.
29022073	1	83	theme	agents	289:294	arg1	activity					263:270	the activity	259:270	the activity of the loosening agents	259:294	Treatment with aluminum triggers a unique response in tea seedlings resulting in biochemical modification of the cell wall, regulation of the activity of the loosening agents, and elongation of root.
29022073	9	84	theme	roots	1855:1859	arg1	length					1841:1846	the length	1837:1846	the length of tea roots	1837:1859	From the results, it can be suggested that the biochemical modification of the cell walls reduces sites of Al binding to roots and triggers the activity of the loosening agents, thereby increasing the length of tea roots.
29022073	4	85	theme	wall	733:736	arg1	properties					710:719	The mechanical properties	695:719	The mechanical properties of the cell wall	695:736	The mechanical properties of the cell wall, the composition of its polysaccharides and their capacity to absorb Al, the expression of genes, and the activities of the wall-modifying proteins were studied.
29022073	4	85	theme	wall	733:736	arg1	activities					844:853	the activities	840:853	the activities of the wall-modifying proteins	840:884	The mechanical properties of the cell wall, the composition of its polysaccharides and their capacity to absorb Al, the expression of genes, and the activities of the wall-modifying proteins were studied.
29022073	4	85	theme	wall	733:736	arg1	composition					743:753	the composition	739:753	the composition of its polysaccharides and their capacity to absorb Al, the expression of genes	739:833	The mechanical properties of the cell wall, the composition of its polysaccharides and their capacity to absorb Al, the expression of genes, and the activities of the wall-modifying proteins were studied.
29022073	3	86	theme	cell	583:586	arg1	wall					588:591	the cell wall	579:591	the cell wall	579:591	A plausible relationship between the modifications of the cell wall and the promotion of root elongation was examined in tea seedlings treated for 8 days with 400 µM Al.
29022073	3	87	theme	root	614:617	arg1	elongation					619:628	root elongation	614:628	root elongation	614:628	A plausible relationship between the modifications of the cell wall and the promotion of root elongation was examined in tea seedlings treated for 8 days with 400 µM Al.
29022073	2	88	theme	terrestrial	333:343	arg1	plants					345:350	most terrestrial plants	328:350	most terrestrial plants	328:350	Unlike most terrestrial plants, tea (Camellia sinensis L.) responds to aluminum (Al) through the promotion of its root elongation; but the real mechanism(s) behind this phenomenon is not well understood.
29022073	1	89	theme	cell	234:237	arg1	wall					239:242	the cell wall	230:242	the cell wall	230:242	Treatment with aluminum triggers a unique response in tea seedlings resulting in biochemical modification of the cell wall, regulation of the activity of the loosening agents, and elongation of root.
25439870	2	0	theme	novel	476:480	arg1	nanofibers					498:507	the novel super-absorbent nanofibers	472:507	the novel super-absorbent nanofibers	472:507	Transmission electron microscopy (TEM) micrographs, X-ray diffraction (XRD) patterns, and Fourier transform infrared (FTIR) analysis of the novel super-absorbent nanofibers suggest the coexistence of PULL, PVA, and MMT through the exfoliation of MMT layers in the super-absorbent nanofiber composite.
25439870	3	1	theme	water	729:733	arg1	absorbency					735:744	a water absorbency	727:744	a water absorbency of 143.42 g g(-1) in distilled water	727:781	The heat-treated PULL/PVA/MMT webs loaded with 5 wt% MMT electrospun nanofibers exhibited a water absorbency of 143.42 g g(-1) in distilled water and a water absorbency of 39.75 g g(-1) in a 0.9 wt% NaCl solution.
25439870	2	2	theme	layers	586:591	arg1	exfoliation					567:577	the exfoliation	563:577	the exfoliation of MMT layers in the super-absorbent nanofiber composite	563:634	Transmission electron microscopy (TEM) micrographs, X-ray diffraction (XRD) patterns, and Fourier transform infrared (FTIR) analysis of the novel super-absorbent nanofibers suggest the coexistence of PULL, PVA, and MMT through the exfoliation of MMT layers in the super-absorbent nanofiber composite.
25439870	5	3	theme	stable	1159:1164	arg1	webs					1147:1150	the webs	1143:1150	the webs highly stable in aqueous environments	1143:1188	The heat treatment improved the crystallinity of the electrospun PULL/PVA/MMT super-absorbent webs and thus made the webs highly stable in aqueous environments.
25439870	3	4	theme	water	789:793	arg1	absorbency					795:804	a water absorbency	787:804	a water absorbency of 39.75 g g(-1) in a 0.9 wt% NaCl solution	787:848	The heat-treated PULL/PVA/MMT webs loaded with 5 wt% MMT electrospun nanofibers exhibited a water absorbency of 143.42 g g(-1) in distilled water and a water absorbency of 39.75 g g(-1) in a 0.9 wt% NaCl solution.
25439870	3	5	theme	MMT	690:692	arg1	nanofibers					706:715	5 wt% MMT electrospun nanofibers	684:715	5 wt% MMT electrospun nanofibers	684:715	The heat-treated PULL/PVA/MMT webs loaded with 5 wt% MMT electrospun nanofibers exhibited a water absorbency of 143.42 g g(-1) in distilled water and a water absorbency of 39.75 g g(-1) in a 0.9 wt% NaCl solution.
25439870	2	6	theme	MMT	582:584	arg1	layers					586:591	MMT layers	582:591	MMT layers in the super-absorbent nanofiber composite	582:634	Transmission electron microscopy (TEM) micrographs, X-ray diffraction (XRD) patterns, and Fourier transform infrared (FTIR) analysis of the novel super-absorbent nanofibers suggest the coexistence of PULL, PVA, and MMT through the exfoliation of MMT layers in the super-absorbent nanofiber composite.
25439870	4	7	theme	saline	965:970	arg1	water					972:976	38% saline water	961:976	38% saline water	961:976	Under extremely dry conditions, the PULL/PVA/MMT webs exhibited the ability to retain 43% distilled water and 38% saline water after being exposed to the atmosphere for one week.
25439870	5	8	theme	super-absorbent	1108:1122	arg1	webs					1124:1127	the electrospun PULL/PVA/MMT super-absorbent webs	1079:1127	the electrospun PULL/PVA/MMT super-absorbent webs	1079:1127	The heat treatment improved the crystallinity of the electrospun PULL/PVA/MMT super-absorbent webs and thus made the webs highly stable in aqueous environments.
25439870	4	9	theme	dry	867:869	arg1	conditions					871:880	extremely dry conditions	857:880	extremely dry conditions	857:880	Under extremely dry conditions, the PULL/PVA/MMT webs exhibited the ability to retain 43% distilled water and 38% saline water after being exposed to the atmosphere for one week.
25439870	3	10	theme	wt	686:687	arg1	nanofibers					706:715	5 wt% MMT electrospun nanofibers	684:715	5 wt% MMT electrospun nanofibers	684:715	The heat-treated PULL/PVA/MMT webs loaded with 5 wt% MMT electrospun nanofibers exhibited a water absorbency of 143.42 g g(-1) in distilled water and a water absorbency of 39.75 g g(-1) in a 0.9 wt% NaCl solution.
25439870	3	11	from	absorbency	795:804	arg1	solution					841:848	a 0.9 wt% NaCl solution	826:848	a 0.9 wt% NaCl solution	826:848	The heat-treated PULL/PVA/MMT webs loaded with 5 wt% MMT electrospun nanofibers exhibited a water absorbency of 143.42 g g(-1) in distilled water and a water absorbency of 39.75 g g(-1) in a 0.9 wt% NaCl solution.
25439870	3	11	from	absorbency	795:804	arg1	water					777:781	distilled water	767:781	distilled water	767:781	The heat-treated PULL/PVA/MMT webs loaded with 5 wt% MMT electrospun nanofibers exhibited a water absorbency of 143.42 g g(-1) in distilled water and a water absorbency of 39.75 g g(-1) in a 0.9 wt% NaCl solution.
25439870	5	12	from	stable	1159:1164	arg1	environments					1177:1188	aqueous environments	1169:1188	aqueous environments	1169:1188	The heat treatment improved the crystallinity of the electrospun PULL/PVA/MMT super-absorbent webs and thus made the webs highly stable in aqueous environments.
25439870	4	13	theme	%	963:963	arg1	water					972:976	38% saline water	961:976	38% saline water	961:976	Under extremely dry conditions, the PULL/PVA/MMT webs exhibited the ability to retain 43% distilled water and 38% saline water after being exposed to the atmosphere for one week.
25439870	5	14	theme	webs	1124:1127	arg1	crystallinity					1062:1074	the crystallinity	1058:1074	the crystallinity of the electrospun PULL/PVA/MMT super-absorbent webs	1058:1127	The heat treatment improved the crystallinity of the electrospun PULL/PVA/MMT super-absorbent webs and thus made the webs highly stable in aqueous environments.
25439870	6	15	theme	mechanical	1263:1272	arg1	properties					1274:1283	mechanical properties	1263:1283	mechanical properties	1263:1283	Overall, the addition of MMT resulted in improved thermal stability and mechanical properties and increased the water absorbency of the PULL/PVA/MMT composite.
25439870	1	16	dep	treated	327:333	arg1	heat					322:325	heat	322:325	heat	322:325	A novel super-absorbent material was fabricated by electrospinning the natural polysaccharide pullulan (PULL) with polyvinyl alcohol (PVA) and montmorillonite (MMT) clay to form nonwoven webs, which were then heat treated.
25439870	1	17	with	pullulan	207:214	arg1	PVA					247:249	PVA	247:249	PVA	247:249	A novel super-absorbent material was fabricated by electrospinning the natural polysaccharide pullulan (PULL) with polyvinyl alcohol (PVA) and montmorillonite (MMT) clay to form nonwoven webs, which were then heat treated.
25439870	1	17	with	pullulan	207:214	arg1	alcohol					238:244	polyvinyl alcohol	228:244	polyvinyl alcohol (PVA)	228:250	A novel super-absorbent material was fabricated by electrospinning the natural polysaccharide pullulan (PULL) with polyvinyl alcohol (PVA) and montmorillonite (MMT) clay to form nonwoven webs, which were then heat treated.
25439870	1	17	with	pullulan	207:214	arg1	clay					278:281	montmorillonite (MMT) clay	256:281	montmorillonite (MMT) clay	256:281	A novel super-absorbent material was fabricated by electrospinning the natural polysaccharide pullulan (PULL) with polyvinyl alcohol (PVA) and montmorillonite (MMT) clay to form nonwoven webs, which were then heat treated.
25439870	3	18	theme	wt	832:833	arg1	solution					841:848	a 0.9 wt% NaCl solution	826:848	a 0.9 wt% NaCl solution	826:848	The heat-treated PULL/PVA/MMT webs loaded with 5 wt% MMT electrospun nanofibers exhibited a water absorbency of 143.42 g g(-1) in distilled water and a water absorbency of 39.75 g g(-1) in a 0.9 wt% NaCl solution.
25439870	1	19	theme	nonwoven	291:298	arg1	webs					300:303	nonwoven webs	291:303	nonwoven webs	291:303	A novel super-absorbent material was fabricated by electrospinning the natural polysaccharide pullulan (PULL) with polyvinyl alcohol (PVA) and montmorillonite (MMT) clay to form nonwoven webs, which were then heat treated.
25439870	2	20	theme	nanofiber	616:624	arg1	composite					626:634	the super-absorbent nanofiber composite	596:634	the super-absorbent nanofiber composite	596:634	Transmission electron microscopy (TEM) micrographs, X-ray diffraction (XRD) patterns, and Fourier transform infrared (FTIR) analysis of the novel super-absorbent nanofibers suggest the coexistence of PULL, PVA, and MMT through the exfoliation of MMT layers in the super-absorbent nanofiber composite.
25439870	2	21	theme	diffraction	394:404	arg1	patterns					412:419	X-ray diffraction (XRD) patterns	388:419	X-ray diffraction (XRD) patterns	388:419	Transmission electron microscopy (TEM) micrographs, X-ray diffraction (XRD) patterns, and Fourier transform infrared (FTIR) analysis of the novel super-absorbent nanofibers suggest the coexistence of PULL, PVA, and MMT through the exfoliation of MMT layers in the super-absorbent nanofiber composite.
25439870	2	22	theme	XRD	407:409	arg1	patterns					412:419	X-ray diffraction (XRD) patterns	388:419	X-ray diffraction (XRD) patterns	388:419	Transmission electron microscopy (TEM) micrographs, X-ray diffraction (XRD) patterns, and Fourier transform infrared (FTIR) analysis of the novel super-absorbent nanofibers suggest the coexistence of PULL, PVA, and MMT through the exfoliation of MMT layers in the super-absorbent nanofiber composite.
25439870	3	23	theme	electrospun	694:704	arg1	nanofibers					706:715	5 wt% MMT electrospun nanofibers	684:715	5 wt% MMT electrospun nanofibers	684:715	The heat-treated PULL/PVA/MMT webs loaded with 5 wt% MMT electrospun nanofibers exhibited a water absorbency of 143.42 g g(-1) in distilled water and a water absorbency of 39.75 g g(-1) in a 0.9 wt% NaCl solution.
25439870	2	24	theme	super-absorbent	600:614	arg1	composite					626:634	the super-absorbent nanofiber composite	596:634	the super-absorbent nanofiber composite	596:634	Transmission electron microscopy (TEM) micrographs, X-ray diffraction (XRD) patterns, and Fourier transform infrared (FTIR) analysis of the novel super-absorbent nanofibers suggest the coexistence of PULL, PVA, and MMT through the exfoliation of MMT layers in the super-absorbent nanofiber composite.
25439870	1	25	theme	novel	115:119	arg1	material					137:144	A novel super-absorbent material	113:144	A novel super-absorbent material	113:144	A novel super-absorbent material was fabricated by electrospinning the natural polysaccharide pullulan (PULL) with polyvinyl alcohol (PVA) and montmorillonite (MMT) clay to form nonwoven webs, which were then heat treated.
25439870	6	26	theme	PULL/PVA/MMT	1327:1338	arg1	composite					1340:1348	the PULL/PVA/MMT composite	1323:1348	the PULL/PVA/MMT composite	1323:1348	Overall, the addition of MMT resulted in improved thermal stability and mechanical properties and increased the water absorbency of the PULL/PVA/MMT composite.
25439870	0	27	theme	novel	12:16	arg1	super-absorbent					18:32	Electrospun novel super-absorbent	0:32	Electrospun novel super-absorbent	0:32	Electrospun novel super-absorbent based on polysaccharide-polyvinyl alcohol-montmorillonite clay nanocomposites.
25439870	1	28	theme	natural	184:190	arg1	pullulan					207:214	the natural polysaccharide pullulan	180:214	the natural polysaccharide pullulan (PULL) with polyvinyl alcohol (PVA) and montmorillonite (MMT) clay	180:281	A novel super-absorbent material was fabricated by electrospinning the natural polysaccharide pullulan (PULL) with polyvinyl alcohol (PVA) and montmorillonite (MMT) clay to form nonwoven webs, which were then heat treated.
25439870	1	28	theme	natural	184:190	arg1	PULL					217:220	PULL	217:220	PULL	217:220	A novel super-absorbent material was fabricated by electrospinning the natural polysaccharide pullulan (PULL) with polyvinyl alcohol (PVA) and montmorillonite (MMT) clay to form nonwoven webs, which were then heat treated.
25439870	4	29	theme	PULL/PVA/MMT	887:898	arg1	webs					900:903	the PULL/PVA/MMT webs	883:903	the PULL/PVA/MMT webs	883:903	Under extremely dry conditions, the PULL/PVA/MMT webs exhibited the ability to retain 43% distilled water and 38% saline water after being exposed to the atmosphere for one week.
25439870	1	30	theme	super-absorbent	121:135	arg1	material					137:144	A novel super-absorbent material	113:144	A novel super-absorbent material	113:144	A novel super-absorbent material was fabricated by electrospinning the natural polysaccharide pullulan (PULL) with polyvinyl alcohol (PVA) and montmorillonite (MMT) clay to form nonwoven webs, which were then heat treated.
25439870	3	31	theme	0.9	828:830	arg1	wt					832:833	wt	832:833	wt	832:833	The heat-treated PULL/PVA/MMT webs loaded with 5 wt% MMT electrospun nanofibers exhibited a water absorbency of 143.42 g g(-1) in distilled water and a water absorbency of 39.75 g g(-1) in a 0.9 wt% NaCl solution.
25439870	1	32	theme	polyvinyl	228:236	arg1	PVA					247:249	PVA	247:249	PVA	247:249	A novel super-absorbent material was fabricated by electrospinning the natural polysaccharide pullulan (PULL) with polyvinyl alcohol (PVA) and montmorillonite (MMT) clay to form nonwoven webs, which were then heat treated.
25439870	1	32	theme	polyvinyl	228:236	arg1	alcohol					238:244	polyvinyl alcohol	228:244	polyvinyl alcohol (PVA)	228:250	A novel super-absorbent material was fabricated by electrospinning the natural polysaccharide pullulan (PULL) with polyvinyl alcohol (PVA) and montmorillonite (MMT) clay to form nonwoven webs, which were then heat treated.
25439870	0	33	theme	Electrospun	0:10	arg1	super-absorbent					18:32	Electrospun novel super-absorbent	0:32	Electrospun novel super-absorbent	0:32	Electrospun novel super-absorbent based on polysaccharide-polyvinyl alcohol-montmorillonite clay nanocomposites.
25439870	5	34	theme	PULL/PVA/MMT	1095:1106	arg1	webs					1124:1127	the electrospun PULL/PVA/MMT super-absorbent webs	1079:1127	the electrospun PULL/PVA/MMT super-absorbent webs	1079:1127	The heat treatment improved the crystallinity of the electrospun PULL/PVA/MMT super-absorbent webs and thus made the webs highly stable in aqueous environments.
25439870	2	35	dep	infrared	444:451	arg1	FTIR					454:457	FTIR	454:457	FTIR	454:457	Transmission electron microscopy (TEM) micrographs, X-ray diffraction (XRD) patterns, and Fourier transform infrared (FTIR) analysis of the novel super-absorbent nanofibers suggest the coexistence of PULL, PVA, and MMT through the exfoliation of MMT layers in the super-absorbent nanofiber composite.
25439870	4	36	theme	43	937:938	arg1	%					939:939	%	939:939	%	939:939	Under extremely dry conditions, the PULL/PVA/MMT webs exhibited the ability to retain 43% distilled water and 38% saline water after being exposed to the atmosphere for one week.
25439870	6	37	theme	improved	1232:1239	arg1	stability					1249:1257	improved thermal stability	1232:1257	improved thermal stability	1232:1257	Overall, the addition of MMT resulted in improved thermal stability and mechanical properties and increased the water absorbency of the PULL/PVA/MMT composite.
25439870	2	38	theme	X-ray	388:392	arg1	patterns					412:419	X-ray diffraction (XRD) patterns	388:419	X-ray diffraction (XRD) patterns	388:419	Transmission electron microscopy (TEM) micrographs, X-ray diffraction (XRD) patterns, and Fourier transform infrared (FTIR) analysis of the novel super-absorbent nanofibers suggest the coexistence of PULL, PVA, and MMT through the exfoliation of MMT layers in the super-absorbent nanofiber composite.
25439870	2	39	from	exfoliation	567:577	arg1	composite					626:634	the super-absorbent nanofiber composite	596:634	the super-absorbent nanofiber composite	596:634	Transmission electron microscopy (TEM) micrographs, X-ray diffraction (XRD) patterns, and Fourier transform infrared (FTIR) analysis of the novel super-absorbent nanofibers suggest the coexistence of PULL, PVA, and MMT through the exfoliation of MMT layers in the super-absorbent nanofiber composite.
25439870	6	40	theme	thermal	1241:1247	arg1	stability					1249:1257	improved thermal stability	1232:1257	improved thermal stability	1232:1257	Overall, the addition of MMT resulted in improved thermal stability and mechanical properties and increased the water absorbency of the PULL/PVA/MMT composite.
25439870	5	41	from	environments	1177:1188	arg1	stable					1159:1164	stable	1159:1164	stable	1159:1164	The heat treatment improved the crystallinity of the electrospun PULL/PVA/MMT super-absorbent webs and thus made the webs highly stable in aqueous environments.
25439870	4	42	theme	distilled	941:949	arg1	water					951:955	43% distilled water	937:955	43% distilled water	937:955	Under extremely dry conditions, the PULL/PVA/MMT webs exhibited the ability to retain 43% distilled water and 38% saline water after being exposed to the atmosphere for one week.
25439870	2	43	from	layers	586:591	arg1	composite					626:634	the super-absorbent nanofiber composite	596:634	the super-absorbent nanofiber composite	596:634	Transmission electron microscopy (TEM) micrographs, X-ray diffraction (XRD) patterns, and Fourier transform infrared (FTIR) analysis of the novel super-absorbent nanofibers suggest the coexistence of PULL, PVA, and MMT through the exfoliation of MMT layers in the super-absorbent nanofiber composite.
25439870	2	44	theme	infrared	444:451	arg1	analysis					460:467	infrared (FTIR) analysis	444:467	infrared (FTIR) analysis of the novel super-absorbent nanofibers	444:507	Transmission electron microscopy (TEM) micrographs, X-ray diffraction (XRD) patterns, and Fourier transform infrared (FTIR) analysis of the novel super-absorbent nanofibers suggest the coexistence of PULL, PVA, and MMT through the exfoliation of MMT layers in the super-absorbent nanofiber composite.
25439870	3	45	from	g	817:817	arg1	solution					841:848	a 0.9 wt% NaCl solution	826:848	a 0.9 wt% NaCl solution	826:848	The heat-treated PULL/PVA/MMT webs loaded with 5 wt% MMT electrospun nanofibers exhibited a water absorbency of 143.42 g g(-1) in distilled water and a water absorbency of 39.75 g g(-1) in a 0.9 wt% NaCl solution.
25439870	3	46	theme	NaCl	836:839	arg1	solution					841:848	a 0.9 wt% NaCl solution	826:848	a 0.9 wt% NaCl solution	826:848	The heat-treated PULL/PVA/MMT webs loaded with 5 wt% MMT electrospun nanofibers exhibited a water absorbency of 143.42 g g(-1) in distilled water and a water absorbency of 39.75 g g(-1) in a 0.9 wt% NaCl solution.
25439870	0	47	theme	polysaccharide-polyvinyl	43:66	arg1	nanocomposites					97:110	polysaccharide-polyvinyl alcohol-montmorillonite clay nanocomposites	43:110	polysaccharide-polyvinyl alcohol-montmorillonite clay nanocomposites	43:110	Electrospun novel super-absorbent based on polysaccharide-polyvinyl alcohol-montmorillonite clay nanocomposites.
25439870	2	48	from	composite	626:634	arg1	exfoliation					567:577	the exfoliation	563:577	the exfoliation of MMT layers in the super-absorbent nanofiber composite	563:634	Transmission electron microscopy (TEM) micrographs, X-ray diffraction (XRD) patterns, and Fourier transform infrared (FTIR) analysis of the novel super-absorbent nanofibers suggest the coexistence of PULL, PVA, and MMT through the exfoliation of MMT layers in the super-absorbent nanofiber composite.
25439870	3	49	theme	heat-treated	641:652	arg1	webs					667:670	The heat-treated PULL/PVA/MMT webs	637:670	The heat-treated PULL/PVA/MMT webs loaded with 5 wt% MMT electrospun nanofibers	637:715	The heat-treated PULL/PVA/MMT webs loaded with 5 wt% MMT electrospun nanofibers exhibited a water absorbency of 143.42 g g(-1) in distilled water and a water absorbency of 39.75 g g(-1) in a 0.9 wt% NaCl solution.
25439870	4	50	theme	%	939:939	arg1	water					951:955	43% distilled water	937:955	43% distilled water	937:955	Under extremely dry conditions, the PULL/PVA/MMT webs exhibited the ability to retain 43% distilled water and 38% saline water after being exposed to the atmosphere for one week.
25439870	5	51	theme	heat	1034:1037	arg1	treatment					1039:1047	The heat treatment	1030:1047	The heat treatment	1030:1047	The heat treatment improved the crystallinity of the electrospun PULL/PVA/MMT super-absorbent webs and thus made the webs highly stable in aqueous environments.
25439870	2	52	theme	PULL	536:539	arg1	PVA					542:544	PVA	542:544	PVA	542:544	Transmission electron microscopy (TEM) micrographs, X-ray diffraction (XRD) patterns, and Fourier transform infrared (FTIR) analysis of the novel super-absorbent nanofibers suggest the coexistence of PULL, PVA, and MMT through the exfoliation of MMT layers in the super-absorbent nanofiber composite.
25439870	2	52	theme	PULL	536:539	arg1	MMT					551:553	MMT	551:553	MMT	551:553	Transmission electron microscopy (TEM) micrographs, X-ray diffraction (XRD) patterns, and Fourier transform infrared (FTIR) analysis of the novel super-absorbent nanofibers suggest the coexistence of PULL, PVA, and MMT through the exfoliation of MMT layers in the super-absorbent nanofiber composite.
25439870	2	52	theme	PULL	536:539	arg1	coexistence					521:531	the coexistence	517:531	the coexistence of PULL	517:539	Transmission electron microscopy (TEM) micrographs, X-ray diffraction (XRD) patterns, and Fourier transform infrared (FTIR) analysis of the novel super-absorbent nanofibers suggest the coexistence of PULL, PVA, and MMT through the exfoliation of MMT layers in the super-absorbent nanofiber composite.
25439870	3	53	theme	%	834:834	arg1	solution					841:848	a 0.9 wt% NaCl solution	826:848	a 0.9 wt% NaCl solution	826:848	The heat-treated PULL/PVA/MMT webs loaded with 5 wt% MMT electrospun nanofibers exhibited a water absorbency of 143.42 g g(-1) in distilled water and a water absorbency of 39.75 g g(-1) in a 0.9 wt% NaCl solution.
25439870	5	54	theme	electrospun	1083:1093	arg1	webs					1124:1127	the electrospun PULL/PVA/MMT super-absorbent webs	1079:1127	the electrospun PULL/PVA/MMT super-absorbent webs	1079:1127	The heat treatment improved the crystallinity of the electrospun PULL/PVA/MMT super-absorbent webs and thus made the webs highly stable in aqueous environments.
25439870	0	55	theme	clay	92:95	arg1	nanocomposites					97:110	polysaccharide-polyvinyl alcohol-montmorillonite clay nanocomposites	43:110	polysaccharide-polyvinyl alcohol-montmorillonite clay nanocomposites	43:110	Electrospun novel super-absorbent based on polysaccharide-polyvinyl alcohol-montmorillonite clay nanocomposites.
25439870	6	56	theme	MMT	1216:1218	arg1	addition					1204:1211	the addition	1200:1211	the addition of MMT	1200:1218	Overall, the addition of MMT resulted in improved thermal stability and mechanical properties and increased the water absorbency of the PULL/PVA/MMT composite.
25439870	3	57	from	absorbency	735:744	arg1	solution					841:848	a 0.9 wt% NaCl solution	826:848	a 0.9 wt% NaCl solution	826:848	The heat-treated PULL/PVA/MMT webs loaded with 5 wt% MMT electrospun nanofibers exhibited a water absorbency of 143.42 g g(-1) in distilled water and a water absorbency of 39.75 g g(-1) in a 0.9 wt% NaCl solution.
25439870	3	57	from	absorbency	735:744	arg1	water					777:781	distilled water	767:781	distilled water	767:781	The heat-treated PULL/PVA/MMT webs loaded with 5 wt% MMT electrospun nanofibers exhibited a water absorbency of 143.42 g g(-1) in distilled water and a water absorbency of 39.75 g g(-1) in a 0.9 wt% NaCl solution.
25439870	0	58	theme	alcohol-montmorillonite	68:90	arg1	nanocomposites					97:110	polysaccharide-polyvinyl alcohol-montmorillonite clay nanocomposites	43:110	polysaccharide-polyvinyl alcohol-montmorillonite clay nanocomposites	43:110	Electrospun novel super-absorbent based on polysaccharide-polyvinyl alcohol-montmorillonite clay nanocomposites.
25439870	3	59	theme	g	756:756	arg1	g					758:758	143.42 g g	749:758	143.42 g g(-1) in distilled water	749:781	The heat-treated PULL/PVA/MMT webs loaded with 5 wt% MMT electrospun nanofibers exhibited a water absorbency of 143.42 g g(-1) in distilled water and a water absorbency of 39.75 g g(-1) in a 0.9 wt% NaCl solution.
25439870	3	59	theme	g	756:756	arg1	-1					760:761	-1	760:761	-1	760:761	The heat-treated PULL/PVA/MMT webs loaded with 5 wt% MMT electrospun nanofibers exhibited a water absorbency of 143.42 g g(-1) in distilled water and a water absorbency of 39.75 g g(-1) in a 0.9 wt% NaCl solution.
25439870	2	60	dep	Fourier	426:432	arg1	transform					434:442	transform	434:442	transform infrared (FTIR) analysis of the novel super-absorbent nanofibers	434:507	Transmission electron microscopy (TEM) micrographs, X-ray diffraction (XRD) patterns, and Fourier transform infrared (FTIR) analysis of the novel super-absorbent nanofibers suggest the coexistence of PULL, PVA, and MMT through the exfoliation of MMT layers in the super-absorbent nanofiber composite.
25439870	2	61	theme	microscopy	358:367	arg1	micrographs					375:385	Transmission electron microscopy (TEM) micrographs	336:385	Transmission electron microscopy (TEM) micrographs	336:385	Transmission electron microscopy (TEM) micrographs, X-ray diffraction (XRD) patterns, and Fourier transform infrared (FTIR) analysis of the novel super-absorbent nanofibers suggest the coexistence of PULL, PVA, and MMT through the exfoliation of MMT layers in the super-absorbent nanofiber composite.
25439870	3	62	theme	g	815:815	arg1	-1					819:820	-1	819:820	-1	819:820	The heat-treated PULL/PVA/MMT webs loaded with 5 wt% MMT electrospun nanofibers exhibited a water absorbency of 143.42 g g(-1) in distilled water and a water absorbency of 39.75 g g(-1) in a 0.9 wt% NaCl solution.
25439870	3	62	theme	g	815:815	arg1	g					817:817	39.75 g g	809:817	39.75 g g(-1) in a 0.9 wt% NaCl solution	809:848	The heat-treated PULL/PVA/MMT webs loaded with 5 wt% MMT electrospun nanofibers exhibited a water absorbency of 143.42 g g(-1) in distilled water and a water absorbency of 39.75 g g(-1) in a 0.9 wt% NaCl solution.
25439870	3	63	theme	g	758:758	arg1	absorbency					735:744	a water absorbency	727:744	a water absorbency of 143.42 g g(-1) in distilled water	727:781	The heat-treated PULL/PVA/MMT webs loaded with 5 wt% MMT electrospun nanofibers exhibited a water absorbency of 143.42 g g(-1) in distilled water and a water absorbency of 39.75 g g(-1) in a 0.9 wt% NaCl solution.
25439870	3	63	theme	g	758:758	arg1	absorbency					795:804	a water absorbency	787:804	a water absorbency of 39.75 g g(-1) in a 0.9 wt% NaCl solution	787:848	The heat-treated PULL/PVA/MMT webs loaded with 5 wt% MMT electrospun nanofibers exhibited a water absorbency of 143.42 g g(-1) in distilled water and a water absorbency of 39.75 g g(-1) in a 0.9 wt% NaCl solution.
25439870	3	64	from	solution	841:848	arg1	absorbency					735:744	a water absorbency	727:744	a water absorbency of 143.42 g g(-1) in distilled water	727:781	The heat-treated PULL/PVA/MMT webs loaded with 5 wt% MMT electrospun nanofibers exhibited a water absorbency of 143.42 g g(-1) in distilled water and a water absorbency of 39.75 g g(-1) in a 0.9 wt% NaCl solution.
25439870	3	64	from	solution	841:848	arg1	absorbency					795:804	a water absorbency	787:804	a water absorbency of 39.75 g g(-1) in a 0.9 wt% NaCl solution	787:848	The heat-treated PULL/PVA/MMT webs loaded with 5 wt% MMT electrospun nanofibers exhibited a water absorbency of 143.42 g g(-1) in distilled water and a water absorbency of 39.75 g g(-1) in a 0.9 wt% NaCl solution.
25439870	2	65	theme	electron	349:356	arg1	microscopy					358:367	Transmission electron microscopy	336:367	Transmission electron microscopy (TEM) micrographs	336:385	Transmission electron microscopy (TEM) micrographs, X-ray diffraction (XRD) patterns, and Fourier transform infrared (FTIR) analysis of the novel super-absorbent nanofibers suggest the coexistence of PULL, PVA, and MMT through the exfoliation of MMT layers in the super-absorbent nanofiber composite.
25439870	2	65	theme	electron	349:356	arg1	TEM					370:372	TEM	370:372	TEM	370:372	Transmission electron microscopy (TEM) micrographs, X-ray diffraction (XRD) patterns, and Fourier transform infrared (FTIR) analysis of the novel super-absorbent nanofibers suggest the coexistence of PULL, PVA, and MMT through the exfoliation of MMT layers in the super-absorbent nanofiber composite.
25439870	3	66	theme	g	817:817	arg1	absorbency					735:744	a water absorbency	727:744	a water absorbency of 143.42 g g(-1) in distilled water	727:781	The heat-treated PULL/PVA/MMT webs loaded with 5 wt% MMT electrospun nanofibers exhibited a water absorbency of 143.42 g g(-1) in distilled water and a water absorbency of 39.75 g g(-1) in a 0.9 wt% NaCl solution.
25439870	3	66	theme	g	817:817	arg1	absorbency					795:804	a water absorbency	787:804	a water absorbency of 39.75 g g(-1) in a 0.9 wt% NaCl solution	787:848	The heat-treated PULL/PVA/MMT webs loaded with 5 wt% MMT electrospun nanofibers exhibited a water absorbency of 143.42 g g(-1) in distilled water and a water absorbency of 39.75 g g(-1) in a 0.9 wt% NaCl solution.
25439870	1	67	theme	montmorillonite	256:270	arg1	clay					278:281	montmorillonite (MMT) clay	256:281	montmorillonite (MMT) clay	256:281	A novel super-absorbent material was fabricated by electrospinning the natural polysaccharide pullulan (PULL) with polyvinyl alcohol (PVA) and montmorillonite (MMT) clay to form nonwoven webs, which were then heat treated.
25439870	3	68	from	water	777:781	arg1	absorbency					735:744	a water absorbency	727:744	a water absorbency of 143.42 g g(-1) in distilled water	727:781	The heat-treated PULL/PVA/MMT webs loaded with 5 wt% MMT electrospun nanofibers exhibited a water absorbency of 143.42 g g(-1) in distilled water and a water absorbency of 39.75 g g(-1) in a 0.9 wt% NaCl solution.
25439870	3	68	from	water	777:781	arg1	absorbency					795:804	a water absorbency	787:804	a water absorbency of 39.75 g g(-1) in a 0.9 wt% NaCl solution	787:848	The heat-treated PULL/PVA/MMT webs loaded with 5 wt% MMT electrospun nanofibers exhibited a water absorbency of 143.42 g g(-1) in distilled water and a water absorbency of 39.75 g g(-1) in a 0.9 wt% NaCl solution.
25439870	3	69	theme	PULL/PVA/MMT	654:665	arg1	webs					667:670	The heat-treated PULL/PVA/MMT webs	637:670	The heat-treated PULL/PVA/MMT webs loaded with 5 wt% MMT electrospun nanofibers	637:715	The heat-treated PULL/PVA/MMT webs loaded with 5 wt% MMT electrospun nanofibers exhibited a water absorbency of 143.42 g g(-1) in distilled water and a water absorbency of 39.75 g g(-1) in a 0.9 wt% NaCl solution.
25439870	6	70	theme	water	1303:1307	arg1	absorbency					1309:1318	the water absorbency	1299:1318	the water absorbency of the PULL/PVA/MMT composite	1299:1348	Overall, the addition of MMT resulted in improved thermal stability and mechanical properties and increased the water absorbency of the PULL/PVA/MMT composite.
25439870	2	71	theme	Transmission	336:347	arg1	microscopy					358:367	Transmission electron microscopy	336:367	Transmission electron microscopy (TEM) micrographs	336:385	Transmission electron microscopy (TEM) micrographs, X-ray diffraction (XRD) patterns, and Fourier transform infrared (FTIR) analysis of the novel super-absorbent nanofibers suggest the coexistence of PULL, PVA, and MMT through the exfoliation of MMT layers in the super-absorbent nanofiber composite.
25439870	2	71	theme	Transmission	336:347	arg1	TEM					370:372	TEM	370:372	TEM	370:372	Transmission electron microscopy (TEM) micrographs, X-ray diffraction (XRD) patterns, and Fourier transform infrared (FTIR) analysis of the novel super-absorbent nanofibers suggest the coexistence of PULL, PVA, and MMT through the exfoliation of MMT layers in the super-absorbent nanofiber composite.
25439870	3	72	from	g	758:758	arg1	water					777:781	distilled water	767:781	distilled water	767:781	The heat-treated PULL/PVA/MMT webs loaded with 5 wt% MMT electrospun nanofibers exhibited a water absorbency of 143.42 g g(-1) in distilled water and a water absorbency of 39.75 g g(-1) in a 0.9 wt% NaCl solution.
25439870	2	73	theme	nanofibers	498:507	arg1	analysis					460:467	infrared (FTIR) analysis	444:467	infrared (FTIR) analysis of the novel super-absorbent nanofibers	444:507	Transmission electron microscopy (TEM) micrographs, X-ray diffraction (XRD) patterns, and Fourier transform infrared (FTIR) analysis of the novel super-absorbent nanofibers suggest the coexistence of PULL, PVA, and MMT through the exfoliation of MMT layers in the super-absorbent nanofiber composite.
25439870	5	74	theme	aqueous	1169:1175	arg1	environments					1177:1188	aqueous environments	1169:1188	aqueous environments	1169:1188	The heat treatment improved the crystallinity of the electrospun PULL/PVA/MMT super-absorbent webs and thus made the webs highly stable in aqueous environments.
25439870	1	75	theme	polysaccharide	192:205	arg1	pullulan					207:214	the natural polysaccharide pullulan	180:214	the natural polysaccharide pullulan (PULL) with polyvinyl alcohol (PVA) and montmorillonite (MMT) clay	180:281	A novel super-absorbent material was fabricated by electrospinning the natural polysaccharide pullulan (PULL) with polyvinyl alcohol (PVA) and montmorillonite (MMT) clay to form nonwoven webs, which were then heat treated.
25439870	1	75	theme	polysaccharide	192:205	arg1	PULL					217:220	PULL	217:220	PULL	217:220	A novel super-absorbent material was fabricated by electrospinning the natural polysaccharide pullulan (PULL) with polyvinyl alcohol (PVA) and montmorillonite (MMT) clay to form nonwoven webs, which were then heat treated.
25439870	1	76	theme	MMT	273:275	arg1	clay					278:281	montmorillonite (MMT) clay	256:281	montmorillonite (MMT) clay	256:281	A novel super-absorbent material was fabricated by electrospinning the natural polysaccharide pullulan (PULL) with polyvinyl alcohol (PVA) and montmorillonite (MMT) clay to form nonwoven webs, which were then heat treated.
25439870	2	77	theme	super-absorbent	482:496	arg1	nanofibers					498:507	the novel super-absorbent nanofibers	472:507	the novel super-absorbent nanofibers	472:507	Transmission electron microscopy (TEM) micrographs, X-ray diffraction (XRD) patterns, and Fourier transform infrared (FTIR) analysis of the novel super-absorbent nanofibers suggest the coexistence of PULL, PVA, and MMT through the exfoliation of MMT layers in the super-absorbent nanofiber composite.
25439870	3	78	theme	%	688:688	arg1	nanofibers					706:715	5 wt% MMT electrospun nanofibers	684:715	5 wt% MMT electrospun nanofibers	684:715	The heat-treated PULL/PVA/MMT webs loaded with 5 wt% MMT electrospun nanofibers exhibited a water absorbency of 143.42 g g(-1) in distilled water and a water absorbency of 39.75 g g(-1) in a 0.9 wt% NaCl solution.
25439870	6	79	theme	composite	1340:1348	arg1	absorbency					1309:1318	the water absorbency	1299:1318	the water absorbency of the PULL/PVA/MMT composite	1299:1348	Overall, the addition of MMT resulted in improved thermal stability and mechanical properties and increased the water absorbency of the PULL/PVA/MMT composite.
25439870	3	80	theme	distilled	767:775	arg1	water					777:781	distilled water	767:781	distilled water	767:781	The heat-treated PULL/PVA/MMT webs loaded with 5 wt% MMT electrospun nanofibers exhibited a water absorbency of 143.42 g g(-1) in distilled water and a water absorbency of 39.75 g g(-1) in a 0.9 wt% NaCl solution.
25439870	4	81	theme	38	961:962	arg1	%					963:963	%	963:963	%	963:963	Under extremely dry conditions, the PULL/PVA/MMT webs exhibited the ability to retain 43% distilled water and 38% saline water after being exposed to the atmosphere for one week.
27261754	0	0	theme	rheology	88:95	arg1	properties					21:30	The physico-chemical properties	0:30	The physico-chemical properties of chia seed polysaccharide and its microgel dispersion rheology	0:95	The physico-chemical properties of chia seed polysaccharide and its microgel dispersion rheology.
27261754	7	1	theme	polysaccharide	1098:1111	arg1	phase					1124:1128	soluble polysaccharide continuous phase	1090:1128	soluble polysaccharide continuous phase	1090:1128	The swollen microgel particles dispersed in soluble polysaccharide continuous phase provided complex and potentially useful rheological properties in food systems.
27261754	4	2	theme	∼700μm	715:720	arg1	D4,3					706:709	D4,3	706:709	D4,3	706:709	The whole polysaccharide (included soluble and insoluble fractions) when dispersed in water showed presence of irregular shape, fibrous microgel particles with an average size (D4,3) of ∼700μm.
27261754	4	2	theme	∼700μm	715:720	arg1	size					700:703	an average size	689:703	an average size (D4,3) of ∼700μm	689:720	The whole polysaccharide (included soluble and insoluble fractions) when dispersed in water showed presence of irregular shape, fibrous microgel particles with an average size (D4,3) of ∼700μm.
27261754	6	3	theme	ionic	966:970	arg1	strengths					972:980	ionic strengths	966:980	ionic strengths (0.01-0.5M NaCl)	966:997	The viscosity of the dispersion was fairly resistant to variations in temperatures (20-80°C), pH (4-12), ionic strengths (0.01-0.5M NaCl) and cation types (MgCl2, CaCl2, NaCl and KCl).
27261754	6	3	theme	ionic	966:970	arg1	NaCl					993:996	0.01-0.5M NaCl	983:996	0.01-0.5M NaCl	983:996	The viscosity of the dispersion was fairly resistant to variations in temperatures (20-80°C), pH (4-12), ionic strengths (0.01-0.5M NaCl) and cation types (MgCl2, CaCl2, NaCl and KCl).
27261754	2	4	theme	insoluble	358:366	arg1	fraction					368:375	negatively charged insoluble fraction	339:375	65% w/w negatively charged insoluble fraction	331:375	The freeze-dried sample consisted of ∼95% non-starch polysaccharides (35% w/w neutral soluble fraction and 65% w/w negatively charged insoluble fraction).
27261754	0	5	theme	dispersion	77:86	arg1	rheology					88:95	its microgel dispersion rheology	64:95	its microgel dispersion rheology	64:95	The physico-chemical properties of chia seed polysaccharide and its microgel dispersion rheology.
27261754	3	6	contain	has	415:417	arg2	mass					425:428	molar mass	419:428	molar mass	419:428	The soluble polysaccharide fraction has molar mass, root-mean square radius and intrinsic viscosity of ∼5×10(5)g/mol, 39nm and 719mL/g, respectively.
27261754	3	6	contain	has	415:417	arg2	719mL/g					506:512	719mL/g	506:512	719mL/g	506:512	The soluble polysaccharide fraction has molar mass, root-mean square radius and intrinsic viscosity of ∼5×10(5)g/mol, 39nm and 719mL/g, respectively.
27261754	3	6	contain	has	415:417	arg2	39nm					497:500	39nm	497:500	39nm	497:500	The soluble polysaccharide fraction has molar mass, root-mean square radius and intrinsic viscosity of ∼5×10(5)g/mol, 39nm and 719mL/g, respectively.
27261754	3	6	contain	has	415:417	arg2	radius					448:453	root-mean square radius	431:453	root-mean square radius	431:453	The soluble polysaccharide fraction has molar mass, root-mean square radius and intrinsic viscosity of ∼5×10(5)g/mol, 39nm and 719mL/g, respectively.
27261754	3	6	contain	has	415:417	arg1	fraction					406:413	The soluble polysaccharide fraction	379:413	The soluble polysaccharide fraction	379:413	The soluble polysaccharide fraction has molar mass, root-mean square radius and intrinsic viscosity of ∼5×10(5)g/mol, 39nm and 719mL/g, respectively.
27261754	2	7	theme	charged	350:356	arg1	fraction					368:375	negatively charged insoluble fraction	339:375	65% w/w negatively charged insoluble fraction	331:375	The freeze-dried sample consisted of ∼95% non-starch polysaccharides (35% w/w neutral soluble fraction and 65% w/w negatively charged insoluble fraction).
27261754	6	8	theme	cation	1003:1008	arg1	types					1010:1014	cation types	1003:1014	cation types (MgCl2, CaCl2, NaCl and KCl)	1003:1043	The viscosity of the dispersion was fairly resistant to variations in temperatures (20-80°C), pH (4-12), ionic strengths (0.01-0.5M NaCl) and cation types (MgCl2, CaCl2, NaCl and KCl).
27261754	6	8	theme	cation	1003:1008	arg1	KCl					1040:1042	KCl	1040:1042	KCl	1040:1042	The viscosity of the dispersion was fairly resistant to variations in temperatures (20-80°C), pH (4-12), ionic strengths (0.01-0.5M NaCl) and cation types (MgCl2, CaCl2, NaCl and KCl).
27261754	6	8	theme	cation	1003:1008	arg1	NaCl					1031:1034	NaCl	1031:1034	NaCl	1031:1034	The viscosity of the dispersion was fairly resistant to variations in temperatures (20-80°C), pH (4-12), ionic strengths (0.01-0.5M NaCl) and cation types (MgCl2, CaCl2, NaCl and KCl).
27261754	6	8	theme	cation	1003:1008	arg1	CaCl2					1024:1028	CaCl2	1024:1028	CaCl2	1024:1028	The viscosity of the dispersion was fairly resistant to variations in temperatures (20-80°C), pH (4-12), ionic strengths (0.01-0.5M NaCl) and cation types (MgCl2, CaCl2, NaCl and KCl).
27261754	6	8	theme	cation	1003:1008	arg1	MgCl2					1017:1021	MgCl2	1017:1021	MgCl2	1017:1021	The viscosity of the dispersion was fairly resistant to variations in temperatures (20-80°C), pH (4-12), ionic strengths (0.01-0.5M NaCl) and cation types (MgCl2, CaCl2, NaCl and KCl).
27261754	2	9	theme	w/w	298:300	arg1	%					296:296	35% w/w neutral soluble fraction and 65% w/w negatively charged insoluble fraction	294:375	%	296:296	The freeze-dried sample consisted of ∼95% non-starch polysaccharides (35% w/w neutral soluble fraction and 65% w/w negatively charged insoluble fraction).
27261754	2	9	theme	w/w	298:300	arg1	polysaccharides					277:291	∼95% non-starch polysaccharides	261:291	∼95% non-starch polysaccharides (35% w/w neutral soluble fraction and 65% w/w negatively charged insoluble fraction)	261:376	The freeze-dried sample consisted of ∼95% non-starch polysaccharides (35% w/w neutral soluble fraction and 65% w/w negatively charged insoluble fraction).
27261754	1	10	theme	gel	117:119	arg1	layer					121:125	The polysaccharide gel layer	98:125	The polysaccharide gel layer surrounding hydrated chia seeds	98:157	The polysaccharide gel layer surrounding hydrated chia seeds was extracted using water and isolated by ethanol precipitation.
27261754	7	11	theme	soluble	1090:1096	arg1	phase					1124:1128	soluble polysaccharide continuous phase	1090:1128	soluble polysaccharide continuous phase	1090:1128	The swollen microgel particles dispersed in soluble polysaccharide continuous phase provided complex and potentially useful rheological properties in food systems.
27261754	3	12	theme	g/mol	490:494	arg1	39nm					497:500	39nm	497:500	39nm	497:500	The soluble polysaccharide fraction has molar mass, root-mean square radius and intrinsic viscosity of ∼5×10(5)g/mol, 39nm and 719mL/g, respectively.
27261754	3	12	theme	g/mol	490:494	arg1	719mL/g					506:512	719mL/g	506:512	719mL/g	506:512	The soluble polysaccharide fraction has molar mass, root-mean square radius and intrinsic viscosity of ∼5×10(5)g/mol, 39nm and 719mL/g, respectively.
27261754	3	12	theme	g/mol	490:494	arg1	radius					448:453	root-mean square radius	431:453	root-mean square radius	431:453	The soluble polysaccharide fraction has molar mass, root-mean square radius and intrinsic viscosity of ∼5×10(5)g/mol, 39nm and 719mL/g, respectively.
27261754	3	12	theme	g/mol	490:494	arg1	viscosity					469:477	intrinsic viscosity	459:477	intrinsic viscosity	459:477	The soluble polysaccharide fraction has molar mass, root-mean square radius and intrinsic viscosity of ∼5×10(5)g/mol, 39nm and 719mL/g, respectively.
27261754	3	12	theme	g/mol	490:494	arg1	mass					425:428	molar mass	419:428	molar mass	419:428	The soluble polysaccharide fraction has molar mass, root-mean square radius and intrinsic viscosity of ∼5×10(5)g/mol, 39nm and 719mL/g, respectively.
27261754	4	13	with	particles	674:682	arg1	D4,3					706:709	D4,3	706:709	D4,3	706:709	The whole polysaccharide (included soluble and insoluble fractions) when dispersed in water showed presence of irregular shape, fibrous microgel particles with an average size (D4,3) of ∼700μm.
27261754	4	13	with	particles	674:682	arg1	size					700:703	an average size	689:703	an average size (D4,3) of ∼700μm	689:720	The whole polysaccharide (included soluble and insoluble fractions) when dispersed in water showed presence of irregular shape, fibrous microgel particles with an average size (D4,3) of ∼700μm.
27261754	5	14	theme	viscoelastic	767:778	arg1	gel					780:782	a 'weak' viscoelastic gel	758:782	a 'weak' viscoelastic gel	758:782	Rheological measurements indicated a 'weak' viscoelastic gel and strong shear dependent properties even at low concentration (0.05% w/w).
27261754	3	15	theme	square	441:446	arg1	radius					448:453	root-mean square radius	431:453	root-mean square radius	431:453	The soluble polysaccharide fraction has molar mass, root-mean square radius and intrinsic viscosity of ∼5×10(5)g/mol, 39nm and 719mL/g, respectively.
27261754	3	16	theme	molar	419:423	arg1	mass					425:428	molar mass	419:428	molar mass	419:428	The soluble polysaccharide fraction has molar mass, root-mean square radius and intrinsic viscosity of ∼5×10(5)g/mol, 39nm and 719mL/g, respectively.
27261754	5	17	theme	w/w	855:857	arg1	concentration					834:846	low concentration	830:846	low concentration (0.05% w/w)	830:858	Rheological measurements indicated a 'weak' viscoelastic gel and strong shear dependent properties even at low concentration (0.05% w/w).
27261754	5	17	theme	w/w	855:857	arg1	%					853:853	0.05% w/w	849:857	0.05% w/w	849:857	Rheological measurements indicated a 'weak' viscoelastic gel and strong shear dependent properties even at low concentration (0.05% w/w).
27261754	5	18	theme	low	830:832	arg1	concentration					834:846	low concentration	830:846	low concentration (0.05% w/w)	830:858	Rheological measurements indicated a 'weak' viscoelastic gel and strong shear dependent properties even at low concentration (0.05% w/w).
27261754	5	18	theme	low	830:832	arg1	%					853:853	0.05% w/w	849:857	0.05% w/w	849:857	Rheological measurements indicated a 'weak' viscoelastic gel and strong shear dependent properties even at low concentration (0.05% w/w).
27261754	0	19	theme	physico-chemical	4:19	arg1	properties					21:30	The physico-chemical properties	0:30	The physico-chemical properties of chia seed polysaccharide and its microgel dispersion rheology	0:95	The physico-chemical properties of chia seed polysaccharide and its microgel dispersion rheology.
27261754	7	20	theme	continuous	1113:1122	arg1	phase					1124:1128	soluble polysaccharide continuous phase	1090:1128	soluble polysaccharide continuous phase	1090:1128	The swollen microgel particles dispersed in soluble polysaccharide continuous phase provided complex and potentially useful rheological properties in food systems.
27261754	2	21	dep	%	296:296	arg1	fraction					318:325	soluble fraction	310:325	soluble fraction	310:325	The freeze-dried sample consisted of ∼95% non-starch polysaccharides (35% w/w neutral soluble fraction and 65% w/w negatively charged insoluble fraction).
27261754	2	22	dep	%	333:333	arg1	fraction					368:375	negatively charged insoluble fraction	339:375	65% w/w negatively charged insoluble fraction	331:375	The freeze-dried sample consisted of ∼95% non-starch polysaccharides (35% w/w neutral soluble fraction and 65% w/w negatively charged insoluble fraction).
27261754	4	23	dep	polysaccharide	539:552	arg1	insoluble					576:584	insoluble	576:584	insoluble	576:584	The whole polysaccharide (included soluble and insoluble fractions) when dispersed in water showed presence of irregular shape, fibrous microgel particles with an average size (D4,3) of ∼700μm.
27261754	4	23	dep	polysaccharide	539:552	arg1	soluble					564:570	soluble	564:570	soluble	564:570	The whole polysaccharide (included soluble and insoluble fractions) when dispersed in water showed presence of irregular shape, fibrous microgel particles with an average size (D4,3) of ∼700μm.
27261754	3	24	theme	polysaccharide	391:404	arg1	fraction					406:413	The soluble polysaccharide fraction	379:413	The soluble polysaccharide fraction	379:413	The soluble polysaccharide fraction has molar mass, root-mean square radius and intrinsic viscosity of ∼5×10(5)g/mol, 39nm and 719mL/g, respectively.
27261754	4	25	theme	whole	533:537	arg1	polysaccharide					539:552	The whole polysaccharide	529:552	The whole polysaccharide (included soluble and insoluble fractions) when dispersed in water	529:619	The whole polysaccharide (included soluble and insoluble fractions) when dispersed in water showed presence of irregular shape, fibrous microgel particles with an average size (D4,3) of ∼700μm.
27261754	4	26	theme	irregular	640:648	arg1	particles					674:682	fibrous microgel particles	657:682	fibrous microgel particles with an average size (D4,3) of ∼700μm	657:720	The whole polysaccharide (included soluble and insoluble fractions) when dispersed in water showed presence of irregular shape, fibrous microgel particles with an average size (D4,3) of ∼700μm.
27261754	4	26	theme	irregular	640:648	arg1	shape					650:654	irregular shape	640:654	irregular shape	640:654	The whole polysaccharide (included soluble and insoluble fractions) when dispersed in water showed presence of irregular shape, fibrous microgel particles with an average size (D4,3) of ∼700μm.
27261754	3	27	theme	root-mean	431:439	arg1	radius					448:453	root-mean square radius	431:453	root-mean square radius	431:453	The soluble polysaccharide fraction has molar mass, root-mean square radius and intrinsic viscosity of ∼5×10(5)g/mol, 39nm and 719mL/g, respectively.
27261754	6	28	dep	types	1010:1014	arg1	KCl					1040:1042	KCl	1040:1042	KCl	1040:1042	The viscosity of the dispersion was fairly resistant to variations in temperatures (20-80°C), pH (4-12), ionic strengths (0.01-0.5M NaCl) and cation types (MgCl2, CaCl2, NaCl and KCl).
27261754	6	28	dep	types	1010:1014	arg1	types					1010:1014	cation types	1003:1014	cation types (MgCl2, CaCl2, NaCl and KCl)	1003:1043	The viscosity of the dispersion was fairly resistant to variations in temperatures (20-80°C), pH (4-12), ionic strengths (0.01-0.5M NaCl) and cation types (MgCl2, CaCl2, NaCl and KCl).
27261754	6	28	dep	types	1010:1014	arg1	NaCl					1031:1034	NaCl	1031:1034	NaCl	1031:1034	The viscosity of the dispersion was fairly resistant to variations in temperatures (20-80°C), pH (4-12), ionic strengths (0.01-0.5M NaCl) and cation types (MgCl2, CaCl2, NaCl and KCl).
27261754	6	28	dep	types	1010:1014	arg1	CaCl2					1024:1028	CaCl2	1024:1028	CaCl2	1024:1028	The viscosity of the dispersion was fairly resistant to variations in temperatures (20-80°C), pH (4-12), ionic strengths (0.01-0.5M NaCl) and cation types (MgCl2, CaCl2, NaCl and KCl).
27261754	6	28	dep	types	1010:1014	arg1	MgCl2					1017:1021	MgCl2	1017:1021	MgCl2	1017:1021	The viscosity of the dispersion was fairly resistant to variations in temperatures (20-80°C), pH (4-12), ionic strengths (0.01-0.5M NaCl) and cation types (MgCl2, CaCl2, NaCl and KCl).
27261754	2	29	theme	soluble	310:316	arg1	fraction					318:325	soluble fraction	310:325	soluble fraction	310:325	The freeze-dried sample consisted of ∼95% non-starch polysaccharides (35% w/w neutral soluble fraction and 65% w/w negatively charged insoluble fraction).
27261754	1	30	theme	ethanol	201:207	arg1	precipitation					209:221	ethanol precipitation	201:221	ethanol precipitation	201:221	The polysaccharide gel layer surrounding hydrated chia seeds was extracted using water and isolated by ethanol precipitation.
27261754	5	31	theme	strong	788:793	arg1	properties					811:820	strong shear dependent properties	788:820	strong shear dependent properties	788:820	Rheological measurements indicated a 'weak' viscoelastic gel and strong shear dependent properties even at low concentration (0.05% w/w).
27261754	2	32	theme	neutral	302:308	arg1	%					296:296	35% w/w neutral soluble fraction and 65% w/w negatively charged insoluble fraction	294:375	%	296:296	The freeze-dried sample consisted of ∼95% non-starch polysaccharides (35% w/w neutral soluble fraction and 65% w/w negatively charged insoluble fraction).
27261754	2	32	theme	neutral	302:308	arg1	polysaccharides					277:291	∼95% non-starch polysaccharides	261:291	∼95% non-starch polysaccharides (35% w/w neutral soluble fraction and 65% w/w negatively charged insoluble fraction)	261:376	The freeze-dried sample consisted of ∼95% non-starch polysaccharides (35% w/w neutral soluble fraction and 65% w/w negatively charged insoluble fraction).
27261754	5	33	theme	dependent	801:809	arg1	properties					811:820	strong shear dependent properties	788:820	strong shear dependent properties	788:820	Rheological measurements indicated a 'weak' viscoelastic gel and strong shear dependent properties even at low concentration (0.05% w/w).
27261754	0	34	theme	seed	40:43	arg1	polysaccharide					45:58	chia seed polysaccharide	35:58	chia seed polysaccharide	35:58	The physico-chemical properties of chia seed polysaccharide and its microgel dispersion rheology.
27261754	7	35	theme	microgel	1058:1065	arg1	particles					1067:1075	The swollen microgel particles	1046:1075	The swollen microgel particles dispersed in soluble polysaccharide continuous phase	1046:1128	The swollen microgel particles dispersed in soluble polysaccharide continuous phase provided complex and potentially useful rheological properties in food systems.
27261754	4	36	theme	shape	650:654	arg1	presence					628:635	presence	628:635	presence of irregular shape, fibrous microgel particles with an average size (D4,3) of ∼700μm	628:720	The whole polysaccharide (included soluble and insoluble fractions) when dispersed in water showed presence of irregular shape, fibrous microgel particles with an average size (D4,3) of ∼700μm.
27261754	0	37	theme	chia	35:38	arg1	polysaccharide					45:58	chia seed polysaccharide	35:58	chia seed polysaccharide	35:58	The physico-chemical properties of chia seed polysaccharide and its microgel dispersion rheology.
27261754	6	38	from	variations	917:926	arg1	20-80°C					945:951	20-80°C	945:951	20-80°C	945:951	The viscosity of the dispersion was fairly resistant to variations in temperatures (20-80°C), pH (4-12), ionic strengths (0.01-0.5M NaCl) and cation types (MgCl2, CaCl2, NaCl and KCl).
27261754	6	38	from	variations	917:926	arg1	CaCl2					1024:1028	CaCl2	1024:1028	CaCl2	1024:1028	The viscosity of the dispersion was fairly resistant to variations in temperatures (20-80°C), pH (4-12), ionic strengths (0.01-0.5M NaCl) and cation types (MgCl2, CaCl2, NaCl and KCl).
27261754	6	38	from	variations	917:926	arg1	strengths					972:980	ionic strengths	966:980	ionic strengths (0.01-0.5M NaCl)	966:997	The viscosity of the dispersion was fairly resistant to variations in temperatures (20-80°C), pH (4-12), ionic strengths (0.01-0.5M NaCl) and cation types (MgCl2, CaCl2, NaCl and KCl).
27261754	6	38	from	variations	917:926	arg1	types					1010:1014	cation types	1003:1014	cation types (MgCl2, CaCl2, NaCl and KCl)	1003:1043	The viscosity of the dispersion was fairly resistant to variations in temperatures (20-80°C), pH (4-12), ionic strengths (0.01-0.5M NaCl) and cation types (MgCl2, CaCl2, NaCl and KCl).
27261754	6	38	from	variations	917:926	arg1	pH					955:956	pH	955:956	pH (4-12)	955:963	The viscosity of the dispersion was fairly resistant to variations in temperatures (20-80°C), pH (4-12), ionic strengths (0.01-0.5M NaCl) and cation types (MgCl2, CaCl2, NaCl and KCl).
27261754	6	38	from	variations	917:926	arg1	NaCl					1031:1034	NaCl	1031:1034	NaCl	1031:1034	The viscosity of the dispersion was fairly resistant to variations in temperatures (20-80°C), pH (4-12), ionic strengths (0.01-0.5M NaCl) and cation types (MgCl2, CaCl2, NaCl and KCl).
27261754	6	38	from	variations	917:926	arg1	4-12					959:962	4-12	959:962	4-12	959:962	The viscosity of the dispersion was fairly resistant to variations in temperatures (20-80°C), pH (4-12), ionic strengths (0.01-0.5M NaCl) and cation types (MgCl2, CaCl2, NaCl and KCl).
27261754	6	38	from	variations	917:926	arg1	KCl					1040:1042	KCl	1040:1042	KCl	1040:1042	The viscosity of the dispersion was fairly resistant to variations in temperatures (20-80°C), pH (4-12), ionic strengths (0.01-0.5M NaCl) and cation types (MgCl2, CaCl2, NaCl and KCl).
27261754	6	38	from	variations	917:926	arg1	temperatures					931:942	temperatures	931:942	temperatures (20-80°C)	931:952	The viscosity of the dispersion was fairly resistant to variations in temperatures (20-80°C), pH (4-12), ionic strengths (0.01-0.5M NaCl) and cation types (MgCl2, CaCl2, NaCl and KCl).
27261754	6	38	from	variations	917:926	arg1	NaCl					993:996	0.01-0.5M NaCl	983:996	0.01-0.5M NaCl	983:996	The viscosity of the dispersion was fairly resistant to variations in temperatures (20-80°C), pH (4-12), ionic strengths (0.01-0.5M NaCl) and cation types (MgCl2, CaCl2, NaCl and KCl).
27261754	6	38	from	variations	917:926	arg1	MgCl2					1017:1021	MgCl2	1017:1021	MgCl2	1017:1021	The viscosity of the dispersion was fairly resistant to variations in temperatures (20-80°C), pH (4-12), ionic strengths (0.01-0.5M NaCl) and cation types (MgCl2, CaCl2, NaCl and KCl).
27261754	4	39	dep	soluble	564:570	arg1	fractions					586:594	fractions	586:594	fractions	586:594	The whole polysaccharide (included soluble and insoluble fractions) when dispersed in water showed presence of irregular shape, fibrous microgel particles with an average size (D4,3) of ∼700μm.
27261754	3	40	theme	intrinsic	459:467	arg1	viscosity					469:477	intrinsic viscosity	459:477	intrinsic viscosity	459:477	The soluble polysaccharide fraction has molar mass, root-mean square radius and intrinsic viscosity of ∼5×10(5)g/mol, 39nm and 719mL/g, respectively.
27261754	5	41	theme	weak	761:764	arg1	gel					780:782	a 'weak' viscoelastic gel	758:782	a 'weak' viscoelastic gel	758:782	Rheological measurements indicated a 'weak' viscoelastic gel and strong shear dependent properties even at low concentration (0.05% w/w).
27261754	4	42	theme	average	692:698	arg1	D4,3					706:709	D4,3	706:709	D4,3	706:709	The whole polysaccharide (included soluble and insoluble fractions) when dispersed in water showed presence of irregular shape, fibrous microgel particles with an average size (D4,3) of ∼700μm.
27261754	4	42	theme	average	692:698	arg1	size					700:703	an average size	689:703	an average size (D4,3) of ∼700μm	689:720	The whole polysaccharide (included soluble and insoluble fractions) when dispersed in water showed presence of irregular shape, fibrous microgel particles with an average size (D4,3) of ∼700μm.
27261754	0	43	theme	polysaccharide	45:58	arg1	properties					21:30	The physico-chemical properties	0:30	The physico-chemical properties of chia seed polysaccharide and its microgel dispersion rheology	0:95	The physico-chemical properties of chia seed polysaccharide and its microgel dispersion rheology.
27261754	6	44	theme	dispersion	882:891	arg1	viscosity					865:873	The viscosity	861:873	The viscosity of the dispersion	861:891	The viscosity of the dispersion was fairly resistant to variations in temperatures (20-80°C), pH (4-12), ionic strengths (0.01-0.5M NaCl) and cation types (MgCl2, CaCl2, NaCl and KCl).
27261754	6	44	theme	dispersion	882:891	arg1	resistant					904:912	resistant	904:912	resistant	904:912	The viscosity of the dispersion was fairly resistant to variations in temperatures (20-80°C), pH (4-12), ionic strengths (0.01-0.5M NaCl) and cation types (MgCl2, CaCl2, NaCl and KCl).
27261754	2	45	theme	freeze-dried	228:239	arg1	sample					241:246	The freeze-dried sample	224:246	The freeze-dried sample	224:246	The freeze-dried sample consisted of ∼95% non-starch polysaccharides (35% w/w neutral soluble fraction and 65% w/w negatively charged insoluble fraction).
27261754	7	46	theme	food	1196:1199	arg1	systems					1201:1207	food systems	1196:1207	food systems	1196:1207	The swollen microgel particles dispersed in soluble polysaccharide continuous phase provided complex and potentially useful rheological properties in food systems.
27261754	7	47	theme	useful	1163:1168	arg1	properties					1182:1191	potentially useful rheological properties	1151:1191	potentially useful rheological properties	1151:1191	The swollen microgel particles dispersed in soluble polysaccharide continuous phase provided complex and potentially useful rheological properties in food systems.
27261754	7	48	theme	swollen	1050:1056	arg1	particles					1067:1075	The swollen microgel particles	1046:1075	The swollen microgel particles dispersed in soluble polysaccharide continuous phase	1046:1128	The swollen microgel particles dispersed in soluble polysaccharide continuous phase provided complex and potentially useful rheological properties in food systems.
27261754	4	49	theme	fibrous	657:663	arg1	particles					674:682	fibrous microgel particles	657:682	fibrous microgel particles with an average size (D4,3) of ∼700μm	657:720	The whole polysaccharide (included soluble and insoluble fractions) when dispersed in water showed presence of irregular shape, fibrous microgel particles with an average size (D4,3) of ∼700μm.
27261754	4	49	theme	fibrous	657:663	arg1	shape					650:654	irregular shape	640:654	irregular shape	640:654	The whole polysaccharide (included soluble and insoluble fractions) when dispersed in water showed presence of irregular shape, fibrous microgel particles with an average size (D4,3) of ∼700μm.
27261754	1	50	theme	hydrated	139:146	arg1	seeds					153:157	hydrated chia seeds	139:157	hydrated chia seeds	139:157	The polysaccharide gel layer surrounding hydrated chia seeds was extracted using water and isolated by ethanol precipitation.
27261754	1	51	theme	polysaccharide	102:115	arg1	layer					121:125	The polysaccharide gel layer	98:125	The polysaccharide gel layer surrounding hydrated chia seeds	98:157	The polysaccharide gel layer surrounding hydrated chia seeds was extracted using water and isolated by ethanol precipitation.
27261754	2	52	theme	non-starch	266:275	arg1	%					296:296	35% w/w neutral soluble fraction and 65% w/w negatively charged insoluble fraction	294:375	%	296:296	The freeze-dried sample consisted of ∼95% non-starch polysaccharides (35% w/w neutral soluble fraction and 65% w/w negatively charged insoluble fraction).
27261754	2	52	theme	non-starch	266:275	arg1	%					333:333	65% w/w	331:337	65% w/w negatively charged insoluble fraction	331:375	The freeze-dried sample consisted of ∼95% non-starch polysaccharides (35% w/w neutral soluble fraction and 65% w/w negatively charged insoluble fraction).
27261754	2	52	theme	non-starch	266:275	arg1	polysaccharides					277:291	∼95% non-starch polysaccharides	261:291	∼95% non-starch polysaccharides (35% w/w neutral soluble fraction and 65% w/w negatively charged insoluble fraction)	261:376	The freeze-dried sample consisted of ∼95% non-starch polysaccharides (35% w/w neutral soluble fraction and 65% w/w negatively charged insoluble fraction).
27261754	1	53	theme	chia	148:151	arg1	seeds					153:157	hydrated chia seeds	139:157	hydrated chia seeds	139:157	The polysaccharide gel layer surrounding hydrated chia seeds was extracted using water and isolated by ethanol precipitation.
27261754	7	54	theme	rheological	1170:1180	arg1	properties					1182:1191	potentially useful rheological properties	1151:1191	potentially useful rheological properties	1151:1191	The swollen microgel particles dispersed in soluble polysaccharide continuous phase provided complex and potentially useful rheological properties in food systems.
27261754	0	55	theme	microgel	68:75	arg1	rheology					88:95	its microgel dispersion rheology	64:95	its microgel dispersion rheology	64:95	The physico-chemical properties of chia seed polysaccharide and its microgel dispersion rheology.
27261754	2	56	theme	%	264:264	arg1	%					296:296	35% w/w neutral soluble fraction and 65% w/w negatively charged insoluble fraction	294:375	%	296:296	The freeze-dried sample consisted of ∼95% non-starch polysaccharides (35% w/w neutral soluble fraction and 65% w/w negatively charged insoluble fraction).
27261754	2	56	theme	%	264:264	arg1	%					333:333	65% w/w	331:337	65% w/w negatively charged insoluble fraction	331:375	The freeze-dried sample consisted of ∼95% non-starch polysaccharides (35% w/w neutral soluble fraction and 65% w/w negatively charged insoluble fraction).
27261754	2	56	theme	%	264:264	arg1	polysaccharides					277:291	∼95% non-starch polysaccharides	261:291	∼95% non-starch polysaccharides (35% w/w neutral soluble fraction and 65% w/w negatively charged insoluble fraction)	261:376	The freeze-dried sample consisted of ∼95% non-starch polysaccharides (35% w/w neutral soluble fraction and 65% w/w negatively charged insoluble fraction).
27261754	4	57	theme	microgel	665:672	arg1	particles					674:682	fibrous microgel particles	657:682	fibrous microgel particles with an average size (D4,3) of ∼700μm	657:720	The whole polysaccharide (included soluble and insoluble fractions) when dispersed in water showed presence of irregular shape, fibrous microgel particles with an average size (D4,3) of ∼700μm.
27261754	4	57	theme	microgel	665:672	arg1	shape					650:654	irregular shape	640:654	irregular shape	640:654	The whole polysaccharide (included soluble and insoluble fractions) when dispersed in water showed presence of irregular shape, fibrous microgel particles with an average size (D4,3) of ∼700μm.
27261754	5	58	theme	Rheological	723:733	arg1	measurements					735:746	Rheological measurements	723:746	Rheological measurements	723:746	Rheological measurements indicated a 'weak' viscoelastic gel and strong shear dependent properties even at low concentration (0.05% w/w).
27261754	2	59	theme	∼95	261:263	arg1	%					264:264	%	264:264	%	264:264	The freeze-dried sample consisted of ∼95% non-starch polysaccharides (35% w/w neutral soluble fraction and 65% w/w negatively charged insoluble fraction).
27261754	3	60	theme	soluble	383:389	arg1	fraction					406:413	The soluble polysaccharide fraction	379:413	The soluble polysaccharide fraction	379:413	The soluble polysaccharide fraction has molar mass, root-mean square radius and intrinsic viscosity of ∼5×10(5)g/mol, 39nm and 719mL/g, respectively.
28681217	0	0	theme	alpha-TCP	102:110	arg1	bone					118:121	alpha-TCP based bone	102:121	alpha-TCP based bone	102:121	How calcite and modified hydroxyapatite influence physicochemical properties and cytocompatibility of alpha-TCP based bone cements.
28681217	8	1	dep	multiphase	1367:1376	arg1	containing					1386:1395	containing	1386:1395	containing MgCHA, AgHA	1386:1407	Results of our research indicate that multiphase cements containing MgCHA, AgHA and calcite combined with αTCP enhanced cell viability in comparison to material based only on αTCP.
28681217	8	1	dep	multiphase	1367:1376	arg1	cements					1378:1384	cements	1378:1384	cements	1378:1384	Results of our research indicate that multiphase cements containing MgCHA, AgHA and calcite combined with αTCP enhanced cell viability in comparison to material based only on αTCP.
28681217	5	2	from	microstructure	846:859	arg1	fluid					905:909	simulated body fluid	890:909	simulated body fluid	890:909	The influence of materials composition on their setting times, microstructure and biochemical stability in simulated body fluid was determined.
28681217	1	3	theme	tissue	181:186	arg1	regeneration					152:163	Nowadays successful regeneration	132:163	Nowadays successful regeneration of damaged bone tissue	132:186	Nowadays successful regeneration of damaged bone tissue is a major problem of the reconstructive medicine and tissue engineering.
28681217	1	3	theme	tissue	181:186	arg1	problem					199:205	a major problem	191:205	a major problem of the reconstructive medicine and tissue engineering	191:259	Nowadays successful regeneration of damaged bone tissue is a major problem of the reconstructive medicine and tissue engineering.
28681217	6	4	theme	metabolic	987:995	arg1	test					1006:1009	metabolic activity test	987:1009	metabolic activity test	987:1009	A number of in vitro laboratory methods, including ICP-OES, metabolic activity test, time-lapse microscopic observation and SEM observations were performed in order to assess biocompatibility of the studied biomaterials.
28681217	7	5	theme	investigated	1225:1236	arg1	composites					1250:1259	all investigated cement-type composites	1221:1259	all investigated cement-type composites	1221:1259	The positive outcome of XTT tests for ceramic extracts demonstrated that all investigated cement-type composites may be considered cytocompatible according to ISO 10993-5 standard.
28681217	5	6	theme	simulated	890:898	arg1	fluid					905:909	simulated body fluid	890:909	simulated body fluid	890:909	The influence of materials composition on their setting times, microstructure and biochemical stability in simulated body fluid was determined.
28681217	3	7	theme	materials	505:513	arg1	properties					479:488	the physicochemical and biological properties	444:488	the physicochemical and biological properties of α-TCP based materials of various phase compositions	444:543	Despite a number of studies regarding CPCs, only a few compare the physicochemical and biological properties of α-TCP based materials of various phase compositions.
28681217	6	8	theme	studied	1126:1132	arg1	biomaterials					1134:1145	the studied biomaterials	1122:1145	the studied biomaterials	1122:1145	A number of in vitro laboratory methods, including ICP-OES, metabolic activity test, time-lapse microscopic observation and SEM observations were performed in order to assess biocompatibility of the studied biomaterials.
28681217	0	9	theme	bone	118:121	arg1	cytocompatibility					81:97	cytocompatibility	81:97	cytocompatibility of alpha-TCP based bone	81:121	How calcite and modified hydroxyapatite influence physicochemical properties and cytocompatibility of alpha-TCP based bone cements.
28681217	0	9	theme	bone	118:121	arg1	properties					66:75	physicochemical properties	50:75	physicochemical properties	50:75	How calcite and modified hydroxyapatite influence physicochemical properties and cytocompatibility of alpha-TCP based bone cements.
28681217	8	10	theme	cell	1449:1452	arg1	viability					1454:1462	cell viability	1449:1462	cell viability	1449:1462	Results of our research indicate that multiphase cements containing MgCHA, AgHA and calcite combined with αTCP enhanced cell viability in comparison to material based only on αTCP.
28681217	4	11	theme	several	585:591	arg1	hydroxyapatite					614:627	hydroxyapatite	614:627	hydroxyapatite doped with Mg2+, CO32- or Ag+ ions	614:662	In our study we compared the effect of several components (calcite, hydroxyapatite doped with Mg2+, CO32- or Ag+ ions, alginate, chitosan and methylcellulose) on the physicochemical and biological properties of α-TCP-based bone cements.
28681217	4	11	theme	several	585:591	arg1	methylcellulose					688:702	methylcellulose	688:702	methylcellulose	688:702	In our study we compared the effect of several components (calcite, hydroxyapatite doped with Mg2+, CO32- or Ag+ ions, alginate, chitosan and methylcellulose) on the physicochemical and biological properties of α-TCP-based bone cements.
28681217	4	11	theme	several	585:591	arg1	components					593:602	several components	585:602	several components (calcite, hydroxyapatite doped with Mg2+, CO32- or Ag+ ions, alginate, chitosan and methylcellulose)	585:703	In our study we compared the effect of several components (calcite, hydroxyapatite doped with Mg2+, CO32- or Ag+ ions, alginate, chitosan and methylcellulose) on the physicochemical and biological properties of α-TCP-based bone cements.
28681217	4	11	theme	several	585:591	arg1	alginate					665:672	alginate	665:672	alginate	665:672	In our study we compared the effect of several components (calcite, hydroxyapatite doped with Mg2+, CO32- or Ag+ ions, alginate, chitosan and methylcellulose) on the physicochemical and biological properties of α-TCP-based bone cements.
28681217	4	11	theme	several	585:591	arg1	calcite					605:611	calcite	605:611	calcite	605:611	In our study we compared the effect of several components (calcite, hydroxyapatite doped with Mg2+, CO32- or Ag+ ions, alginate, chitosan and methylcellulose) on the physicochemical and biological properties of α-TCP-based bone cements.
28681217	4	11	theme	several	585:591	arg1	chitosan					675:682	chitosan	675:682	chitosan	675:682	In our study we compared the effect of several components (calcite, hydroxyapatite doped with Mg2+, CO32- or Ag+ ions, alginate, chitosan and methylcellulose) on the physicochemical and biological properties of α-TCP-based bone cements.
28681217	0	12	theme	based	112:116	arg1	bone					118:121	alpha-TCP based bone	102:121	alpha-TCP based bone	102:121	How calcite and modified hydroxyapatite influence physicochemical properties and cytocompatibility of alpha-TCP based bone cements.
28681217	4	13	theme	biological	732:741	arg1	properties					743:752	the physicochemical and biological properties	708:752	the physicochemical and biological properties of α-TCP-based bone cements	708:780	In our study we compared the effect of several components (calcite, hydroxyapatite doped with Mg2+, CO32- or Ag+ ions, alginate, chitosan and methylcellulose) on the physicochemical and biological properties of α-TCP-based bone cements.
28681217	5	14	from	stability	877:885	arg1	fluid					905:909	simulated body fluid	890:909	simulated body fluid	890:909	The influence of materials composition on their setting times, microstructure and biochemical stability in simulated body fluid was determined.
28681217	2	15	theme	effective	357:365	arg1	fillers					372:378	the effective bone fillers	353:378	the effective bone fillers	353:378	Recently a great deal of attention has been focused on calcium phosphate cements (CPCs) as the effective bone fillers.
28681217	5	16	theme	setting	831:837	arg1	times					839:843	their setting times	825:843	their setting times	825:843	The influence of materials composition on their setting times, microstructure and biochemical stability in simulated body fluid was determined.
28681217	4	17	from	effect	575:580	arg1	properties					743:752	the physicochemical and biological properties	708:752	the physicochemical and biological properties of α-TCP-based bone cements	708:780	In our study we compared the effect of several components (calcite, hydroxyapatite doped with Mg2+, CO32- or Ag+ ions, alginate, chitosan and methylcellulose) on the physicochemical and biological properties of α-TCP-based bone cements.
28681217	6	18	theme	biomaterials	1134:1145	arg1	biocompatibility					1102:1117	biocompatibility	1102:1117	biocompatibility of the studied biomaterials	1102:1145	A number of in vitro laboratory methods, including ICP-OES, metabolic activity test, time-lapse microscopic observation and SEM observations were performed in order to assess biocompatibility of the studied biomaterials.
28681217	3	19	theme	phase	526:530	arg1	compositions					532:543	various phase compositions	518:543	various phase compositions	518:543	Despite a number of studies regarding CPCs, only a few compare the physicochemical and biological properties of α-TCP based materials of various phase compositions.
28681217	4	20	theme	CO32-	646:650	arg1	ions					659:662	Mg2+, CO32- or Ag+ ions	640:662	Mg2+, CO32- or Ag+ ions	640:662	In our study we compared the effect of several components (calcite, hydroxyapatite doped with Mg2+, CO32- or Ag+ ions, alginate, chitosan and methylcellulose) on the physicochemical and biological properties of α-TCP-based bone cements.
28681217	3	21	theme	studies	401:407	arg1	number					391:396	a number	389:396	a number of studies regarding CPCs	389:422	Despite a number of studies regarding CPCs, only a few compare the physicochemical and biological properties of α-TCP based materials of various phase compositions.
28681217	5	22	theme	body	900:903	arg1	fluid					905:909	simulated body fluid	890:909	simulated body fluid	890:909	The influence of materials composition on their setting times, microstructure and biochemical stability in simulated body fluid was determined.
28681217	7	23	theme	positive	1152:1159	arg1	outcome					1161:1167	The positive outcome	1148:1167	The positive outcome of XTT tests for ceramic extracts	1148:1201	The positive outcome of XTT tests for ceramic extracts demonstrated that all investigated cement-type composites may be considered cytocompatible according to ISO 10993-5 standard.
28681217	2	24	theme	attention	287:295	arg1	deal					279:282	a great deal	271:282	a great deal of attention	271:295	Recently a great deal of attention has been focused on calcium phosphate cements (CPCs) as the effective bone fillers.
28681217	1	25	theme	major	193:197	arg1	problem					199:205	a major problem	191:205	a major problem of the reconstructive medicine and tissue engineering	191:259	Nowadays successful regeneration of damaged bone tissue is a major problem of the reconstructive medicine and tissue engineering.
28681217	1	25	theme	major	193:197	arg1	regeneration					152:163	Nowadays successful regeneration	132:163	Nowadays successful regeneration of damaged bone tissue	132:186	Nowadays successful regeneration of damaged bone tissue is a major problem of the reconstructive medicine and tissue engineering.
28681217	7	26	theme	tests	1176:1180	arg1	outcome					1161:1167	The positive outcome	1148:1167	The positive outcome of XTT tests for ceramic extracts	1148:1201	The positive outcome of XTT tests for ceramic extracts demonstrated that all investigated cement-type composites may be considered cytocompatible according to ISO 10993-5 standard.
28681217	3	27	theme	various	518:524	arg1	compositions					532:543	various phase compositions	518:543	various phase compositions	518:543	Despite a number of studies regarding CPCs, only a few compare the physicochemical and biological properties of α-TCP based materials of various phase compositions.
28681217	7	28	theme	standard	1319:1326	arg1	ISO					1307:1309	ISO	1307:1309	ISO 10993-5 standard	1307:1326	The positive outcome of XTT tests for ceramic extracts demonstrated that all investigated cement-type composites may be considered cytocompatible according to ISO 10993-5 standard.
28681217	9	29	theme	higher	1582:1587	arg1	cytocompatibility					1589:1605	higher cytocompatibility	1582:1605	higher cytocompatibility	1582:1605	Furthermore materials containing chitosan and methylcellulose possessed higher cytocompatibility than those with alginate.
28681217	4	30	theme	Ag+	655:657	arg1	ions					659:662	Mg2+, CO32- or Ag+ ions	640:662	Mg2+, CO32- or Ag+ ions	640:662	In our study we compared the effect of several components (calcite, hydroxyapatite doped with Mg2+, CO32- or Ag+ ions, alginate, chitosan and methylcellulose) on the physicochemical and biological properties of α-TCP-based bone cements.
28681217	9	31	contain	containing	1532:1541	arg1	materials					1522:1530	materials	1522:1530	materials containing chitosan and methylcellulose	1522:1570	Furthermore materials containing chitosan and methylcellulose possessed higher cytocompatibility than those with alginate.
28681217	9	31	contain	containing	1532:1541	arg2	methylcellulose					1556:1570	methylcellulose	1556:1570	methylcellulose	1556:1570	Furthermore materials containing chitosan and methylcellulose possessed higher cytocompatibility than those with alginate.
28681217	9	31	contain	containing	1532:1541	arg2	chitosan					1543:1550	chitosan	1543:1550	chitosan	1543:1550	Furthermore materials containing chitosan and methylcellulose possessed higher cytocompatibility than those with alginate.
28681217	4	32	dep	bone	769:772	arg1	cements					774:780	cements	774:780	cements	774:780	In our study we compared the effect of several components (calcite, hydroxyapatite doped with Mg2+, CO32- or Ag+ ions, alginate, chitosan and methylcellulose) on the physicochemical and biological properties of α-TCP-based bone cements.
28681217	0	33	theme	modified	16:23	arg1	hydroxyapatite					25:38	modified hydroxyapatite	16:38	modified hydroxyapatite	16:38	How calcite and modified hydroxyapatite influence physicochemical properties and cytocompatibility of alpha-TCP based bone cements.
28681217	6	34	theme	methods	959:965	arg1	ICP-OES					978:984	ICP-OES	978:984	ICP-OES	978:984	A number of in vitro laboratory methods, including ICP-OES, metabolic activity test, time-lapse microscopic observation and SEM observations were performed in order to assess biocompatibility of the studied biomaterials.
28681217	6	34	theme	methods	959:965	arg1	test					1006:1009	metabolic activity test	987:1009	metabolic activity test	987:1009	A number of in vitro laboratory methods, including ICP-OES, metabolic activity test, time-lapse microscopic observation and SEM observations were performed in order to assess biocompatibility of the studied biomaterials.
28681217	6	34	theme	methods	959:965	arg1	SEM					1051:1053	SEM	1051:1053	SEM	1051:1053	A number of in vitro laboratory methods, including ICP-OES, metabolic activity test, time-lapse microscopic observation and SEM observations were performed in order to assess biocompatibility of the studied biomaterials.
28681217	6	34	theme	methods	959:965	arg1	number					929:934	A number	927:934	A number of in vitro laboratory methods, including ICP-OES, metabolic activity test, time-lapse microscopic observation and SEM observations	927:1066	A number of in vitro laboratory methods, including ICP-OES, metabolic activity test, time-lapse microscopic observation and SEM observations were performed in order to assess biocompatibility of the studied biomaterials.
28681217	6	34	theme	methods	959:965	arg1	observation					1035:1045	time-lapse microscopic observation	1012:1045	time-lapse microscopic observation	1012:1045	A number of in vitro laboratory methods, including ICP-OES, metabolic activity test, time-lapse microscopic observation and SEM observations were performed in order to assess biocompatibility of the studied biomaterials.
28681217	2	35	theme	calcium	317:323	arg1	phosphate					325:333	calcium phosphate cements	317:341	calcium phosphate cements (CPCs) as the effective bone fillers	317:378	Recently a great deal of attention has been focused on calcium phosphate cements (CPCs) as the effective bone fillers.
28681217	2	35	theme	calcium	317:323	arg1	CPCs					344:347	CPCs	344:347	CPCs	344:347	Recently a great deal of attention has been focused on calcium phosphate cements (CPCs) as the effective bone fillers.
28681217	3	36	theme	physicochemical	448:462	arg1	properties					479:488	the physicochemical and biological properties	444:488	the physicochemical and biological properties of α-TCP based materials of various phase compositions	444:543	Despite a number of studies regarding CPCs, only a few compare the physicochemical and biological properties of α-TCP based materials of various phase compositions.
28681217	5	37	theme	composition	810:820	arg1	influence					787:795	The influence	783:795	The influence of materials composition on their setting times, microstructure and biochemical stability in simulated body fluid	783:909	The influence of materials composition on their setting times, microstructure and biochemical stability in simulated body fluid was determined.
28681217	6	38	theme	laboratory	948:957	arg1	test					1006:1009	metabolic activity test	987:1009	metabolic activity test	987:1009	A number of in vitro laboratory methods, including ICP-OES, metabolic activity test, time-lapse microscopic observation and SEM observations were performed in order to assess biocompatibility of the studied biomaterials.
28681217	6	38	theme	laboratory	948:957	arg1	SEM					1051:1053	SEM	1051:1053	SEM	1051:1053	A number of in vitro laboratory methods, including ICP-OES, metabolic activity test, time-lapse microscopic observation and SEM observations were performed in order to assess biocompatibility of the studied biomaterials.
28681217	6	38	theme	laboratory	948:957	arg1	methods					959:965	in vitro laboratory methods	939:965	in vitro laboratory methods	939:965	A number of in vitro laboratory methods, including ICP-OES, metabolic activity test, time-lapse microscopic observation and SEM observations were performed in order to assess biocompatibility of the studied biomaterials.
28681217	6	38	theme	laboratory	948:957	arg1	observation					1035:1045	time-lapse microscopic observation	1012:1045	time-lapse microscopic observation	1012:1045	A number of in vitro laboratory methods, including ICP-OES, metabolic activity test, time-lapse microscopic observation and SEM observations were performed in order to assess biocompatibility of the studied biomaterials.
28681217	6	38	theme	laboratory	948:957	arg1	ICP-OES					978:984	ICP-OES	978:984	ICP-OES	978:984	A number of in vitro laboratory methods, including ICP-OES, metabolic activity test, time-lapse microscopic observation and SEM observations were performed in order to assess biocompatibility of the studied biomaterials.
28681217	4	39	theme	α-TCP-based	757:767	arg1	bone					769:772	α-TCP-based bone cements	757:780	α-TCP-based bone cements	757:780	In our study we compared the effect of several components (calcite, hydroxyapatite doped with Mg2+, CO32- or Ag+ ions, alginate, chitosan and methylcellulose) on the physicochemical and biological properties of α-TCP-based bone cements.
28681217	4	40	theme	physicochemical	712:726	arg1	properties					743:752	the physicochemical and biological properties	708:752	the physicochemical and biological properties of α-TCP-based bone cements	708:780	In our study we compared the effect of several components (calcite, hydroxyapatite doped with Mg2+, CO32- or Ag+ ions, alginate, chitosan and methylcellulose) on the physicochemical and biological properties of α-TCP-based bone cements.
28681217	1	41	theme	reconstructive	214:227	arg1	medicine					229:236	the reconstructive medicine	210:236	the reconstructive medicine	210:236	Nowadays successful regeneration of damaged bone tissue is a major problem of the reconstructive medicine and tissue engineering.
28681217	3	42	theme	based	499:503	arg1	materials					505:513	α-TCP based materials	493:513	α-TCP based materials of various phase compositions	493:543	Despite a number of studies regarding CPCs, only a few compare the physicochemical and biological properties of α-TCP based materials of various phase compositions.
28681217	3	43	theme	α-TCP	493:497	arg1	materials					505:513	α-TCP based materials	493:513	α-TCP based materials of various phase compositions	493:543	Despite a number of studies regarding CPCs, only a few compare the physicochemical and biological properties of α-TCP based materials of various phase compositions.
28681217	7	44	theme	ceramic	1186:1192	arg1	extracts					1194:1201	ceramic extracts	1186:1201	ceramic extracts	1186:1201	The positive outcome of XTT tests for ceramic extracts demonstrated that all investigated cement-type composites may be considered cytocompatible according to ISO 10993-5 standard.
28681217	4	45	theme	Mg2+	640:643	arg1	ions					659:662	Mg2+, CO32- or Ag+ ions	640:662	Mg2+, CO32- or Ag+ ions	640:662	In our study we compared the effect of several components (calcite, hydroxyapatite doped with Mg2+, CO32- or Ag+ ions, alginate, chitosan and methylcellulose) on the physicochemical and biological properties of α-TCP-based bone cements.
28681217	8	46	theme	research	1344:1351	arg1	Results					1329:1335	Results	1329:1335	Results of our research	1329:1351	Results of our research indicate that multiphase cements containing MgCHA, AgHA and calcite combined with αTCP enhanced cell viability in comparison to material based only on αTCP.
28681217	5	47	theme	biochemical	865:875	arg1	stability					877:885	biochemical stability	865:885	biochemical stability	865:885	The influence of materials composition on their setting times, microstructure and biochemical stability in simulated body fluid was determined.
28681217	1	48	theme	medicine	229:236	arg1	problem					199:205	a major problem	191:205	a major problem of the reconstructive medicine and tissue engineering	191:259	Nowadays successful regeneration of damaged bone tissue is a major problem of the reconstructive medicine and tissue engineering.
28681217	1	48	theme	medicine	229:236	arg1	regeneration					152:163	Nowadays successful regeneration	132:163	Nowadays successful regeneration of damaged bone tissue	132:186	Nowadays successful regeneration of damaged bone tissue is a major problem of the reconstructive medicine and tissue engineering.
28681217	6	49	theme	time-lapse	1012:1021	arg1	observation					1035:1045	time-lapse microscopic observation	1012:1045	time-lapse microscopic observation	1012:1045	A number of in vitro laboratory methods, including ICP-OES, metabolic activity test, time-lapse microscopic observation and SEM observations were performed in order to assess biocompatibility of the studied biomaterials.
28681217	6	50	theme	in	939:940	arg1	test					1006:1009	metabolic activity test	987:1009	metabolic activity test	987:1009	A number of in vitro laboratory methods, including ICP-OES, metabolic activity test, time-lapse microscopic observation and SEM observations were performed in order to assess biocompatibility of the studied biomaterials.
28681217	6	50	theme	in	939:940	arg1	SEM					1051:1053	SEM	1051:1053	SEM	1051:1053	A number of in vitro laboratory methods, including ICP-OES, metabolic activity test, time-lapse microscopic observation and SEM observations were performed in order to assess biocompatibility of the studied biomaterials.
28681217	6	50	theme	in	939:940	arg1	methods					959:965	in vitro laboratory methods	939:965	in vitro laboratory methods	939:965	A number of in vitro laboratory methods, including ICP-OES, metabolic activity test, time-lapse microscopic observation and SEM observations were performed in order to assess biocompatibility of the studied biomaterials.
28681217	6	50	theme	in	939:940	arg1	observation					1035:1045	time-lapse microscopic observation	1012:1045	time-lapse microscopic observation	1012:1045	A number of in vitro laboratory methods, including ICP-OES, metabolic activity test, time-lapse microscopic observation and SEM observations were performed in order to assess biocompatibility of the studied biomaterials.
28681217	6	50	theme	in	939:940	arg1	ICP-OES					978:984	ICP-OES	978:984	ICP-OES	978:984	A number of in vitro laboratory methods, including ICP-OES, metabolic activity test, time-lapse microscopic observation and SEM observations were performed in order to assess biocompatibility of the studied biomaterials.
28681217	6	51	dep	in	939:940	arg1	vitro					942:946	vitro	942:946	vitro	942:946	A number of in vitro laboratory methods, including ICP-OES, metabolic activity test, time-lapse microscopic observation and SEM observations were performed in order to assess biocompatibility of the studied biomaterials.
28681217	5	52	theme	materials	800:808	arg1	composition					810:820	materials composition	800:820	materials composition	800:820	The influence of materials composition on their setting times, microstructure and biochemical stability in simulated body fluid was determined.
28681217	6	53	theme	microscopic	1023:1033	arg1	observation					1035:1045	time-lapse microscopic observation	1012:1045	time-lapse microscopic observation	1012:1045	A number of in vitro laboratory methods, including ICP-OES, metabolic activity test, time-lapse microscopic observation and SEM observations were performed in order to assess biocompatibility of the studied biomaterials.
28681217	5	54	from	times	839:843	arg1	fluid					905:909	simulated body fluid	890:909	simulated body fluid	890:909	The influence of materials composition on their setting times, microstructure and biochemical stability in simulated body fluid was determined.
28681217	1	55	theme	Nowadays	132:139	arg1	regeneration					152:163	Nowadays successful regeneration	132:163	Nowadays successful regeneration of damaged bone tissue	132:186	Nowadays successful regeneration of damaged bone tissue is a major problem of the reconstructive medicine and tissue engineering.
28681217	1	55	theme	Nowadays	132:139	arg1	problem					199:205	a major problem	191:205	a major problem of the reconstructive medicine and tissue engineering	191:259	Nowadays successful regeneration of damaged bone tissue is a major problem of the reconstructive medicine and tissue engineering.
28681217	0	56	theme	physicochemical	50:64	arg1	properties					66:75	physicochemical properties	50:75	physicochemical properties	50:75	How calcite and modified hydroxyapatite influence physicochemical properties and cytocompatibility of alpha-TCP based bone cements.
28681217	8	57	dep	material	1481:1488	arg1	comparison					1467:1476	comparison	1467:1476	comparison	1467:1476	Results of our research indicate that multiphase cements containing MgCHA, AgHA and calcite combined with αTCP enhanced cell viability in comparison to material based only on αTCP.
28681217	1	58	theme	bone	176:179	arg1	tissue					181:186	damaged bone tissue	168:186	damaged bone tissue	168:186	Nowadays successful regeneration of damaged bone tissue is a major problem of the reconstructive medicine and tissue engineering.
28681217	1	59	theme	successful	141:150	arg1	regeneration					152:163	Nowadays successful regeneration	132:163	Nowadays successful regeneration of damaged bone tissue	132:186	Nowadays successful regeneration of damaged bone tissue is a major problem of the reconstructive medicine and tissue engineering.
28681217	1	59	theme	successful	141:150	arg1	problem					199:205	a major problem	191:205	a major problem of the reconstructive medicine and tissue engineering	191:259	Nowadays successful regeneration of damaged bone tissue is a major problem of the reconstructive medicine and tissue engineering.
28681217	5	60	from	influence	787:795	arg1	microstructure					846:859	microstructure	846:859	microstructure	846:859	The influence of materials composition on their setting times, microstructure and biochemical stability in simulated body fluid was determined.
28681217	5	60	from	influence	787:795	arg1	stability					877:885	biochemical stability	865:885	biochemical stability	865:885	The influence of materials composition on their setting times, microstructure and biochemical stability in simulated body fluid was determined.
28681217	5	60	from	influence	787:795	arg1	times					839:843	their setting times	825:843	their setting times	825:843	The influence of materials composition on their setting times, microstructure and biochemical stability in simulated body fluid was determined.
28681217	9	61	contain	possessed	1572:1580	arg2	cytocompatibility					1589:1605	higher cytocompatibility	1582:1605	higher cytocompatibility	1582:1605	Furthermore materials containing chitosan and methylcellulose possessed higher cytocompatibility than those with alginate.
28681217	9	61	contain	possessed	1572:1580	arg1	materials					1522:1530	materials	1522:1530	materials containing chitosan and methylcellulose	1522:1570	Furthermore materials containing chitosan and methylcellulose possessed higher cytocompatibility than those with alginate.
28681217	3	62	theme	biological	468:477	arg1	properties					479:488	the physicochemical and biological properties	444:488	the physicochemical and biological properties of α-TCP based materials of various phase compositions	444:543	Despite a number of studies regarding CPCs, only a few compare the physicochemical and biological properties of α-TCP based materials of various phase compositions.
28681217	1	63	theme	tissue	242:247	arg1	engineering					249:259	tissue engineering	242:259	tissue engineering	242:259	Nowadays successful regeneration of damaged bone tissue is a major problem of the reconstructive medicine and tissue engineering.
28681217	3	64	theme	compositions	532:543	arg1	materials					505:513	α-TCP based materials	493:513	α-TCP based materials of various phase compositions	493:543	Despite a number of studies regarding CPCs, only a few compare the physicochemical and biological properties of α-TCP based materials of various phase compositions.
28681217	4	65	theme	bone	769:772	arg1	properties					743:752	the physicochemical and biological properties	708:752	the physicochemical and biological properties of α-TCP-based bone cements	708:780	In our study we compared the effect of several components (calcite, hydroxyapatite doped with Mg2+, CO32- or Ag+ ions, alginate, chitosan and methylcellulose) on the physicochemical and biological properties of α-TCP-based bone cements.
28681217	2	66	dep	phosphate	325:333	arg1	cements					335:341	cements	335:341	cements	335:341	Recently a great deal of attention has been focused on calcium phosphate cements (CPCs) as the effective bone fillers.
28681217	1	67	theme	engineering	249:259	arg1	problem					199:205	a major problem	191:205	a major problem of the reconstructive medicine and tissue engineering	191:259	Nowadays successful regeneration of damaged bone tissue is a major problem of the reconstructive medicine and tissue engineering.
28681217	1	67	theme	engineering	249:259	arg1	regeneration					152:163	Nowadays successful regeneration	132:163	Nowadays successful regeneration of damaged bone tissue	132:186	Nowadays successful regeneration of damaged bone tissue is a major problem of the reconstructive medicine and tissue engineering.
28681217	7	68	theme	cement-type	1238:1248	arg1	composites					1250:1259	all investigated cement-type composites	1221:1259	all investigated cement-type composites	1221:1259	The positive outcome of XTT tests for ceramic extracts demonstrated that all investigated cement-type composites may be considered cytocompatible according to ISO 10993-5 standard.
28681217	4	69	dep	components	593:602	arg1	hydroxyapatite					614:627	hydroxyapatite	614:627	hydroxyapatite doped with Mg2+, CO32- or Ag+ ions	614:662	In our study we compared the effect of several components (calcite, hydroxyapatite doped with Mg2+, CO32- or Ag+ ions, alginate, chitosan and methylcellulose) on the physicochemical and biological properties of α-TCP-based bone cements.
28681217	4	69	dep	components	593:602	arg1	methylcellulose					688:702	methylcellulose	688:702	methylcellulose	688:702	In our study we compared the effect of several components (calcite, hydroxyapatite doped with Mg2+, CO32- or Ag+ ions, alginate, chitosan and methylcellulose) on the physicochemical and biological properties of α-TCP-based bone cements.
28681217	4	69	dep	components	593:602	arg1	components					593:602	several components	585:602	several components (calcite, hydroxyapatite doped with Mg2+, CO32- or Ag+ ions, alginate, chitosan and methylcellulose)	585:703	In our study we compared the effect of several components (calcite, hydroxyapatite doped with Mg2+, CO32- or Ag+ ions, alginate, chitosan and methylcellulose) on the physicochemical and biological properties of α-TCP-based bone cements.
28681217	4	69	dep	components	593:602	arg1	alginate					665:672	alginate	665:672	alginate	665:672	In our study we compared the effect of several components (calcite, hydroxyapatite doped with Mg2+, CO32- or Ag+ ions, alginate, chitosan and methylcellulose) on the physicochemical and biological properties of α-TCP-based bone cements.
28681217	4	69	dep	components	593:602	arg1	calcite					605:611	calcite	605:611	calcite	605:611	In our study we compared the effect of several components (calcite, hydroxyapatite doped with Mg2+, CO32- or Ag+ ions, alginate, chitosan and methylcellulose) on the physicochemical and biological properties of α-TCP-based bone cements.
28681217	4	69	dep	components	593:602	arg1	chitosan					675:682	chitosan	675:682	chitosan	675:682	In our study we compared the effect of several components (calcite, hydroxyapatite doped with Mg2+, CO32- or Ag+ ions, alginate, chitosan and methylcellulose) on the physicochemical and biological properties of α-TCP-based bone cements.
28681217	2	70	theme	great	273:277	arg1	deal					279:282	a great deal	271:282	a great deal of attention	271:295	Recently a great deal of attention has been focused on calcium phosphate cements (CPCs) as the effective bone fillers.
28681217	1	71	theme	damaged	168:174	arg1	tissue					181:186	damaged bone tissue	168:186	damaged bone tissue	168:186	Nowadays successful regeneration of damaged bone tissue is a major problem of the reconstructive medicine and tissue engineering.
28681217	6	72	dep	ICP-OES	978:984	arg1	observations					1055:1066	observations	1055:1066	observations	1055:1066	A number of in vitro laboratory methods, including ICP-OES, metabolic activity test, time-lapse microscopic observation and SEM observations were performed in order to assess biocompatibility of the studied biomaterials.
28681217	7	73	theme	XTT	1172:1174	arg1	tests					1176:1180	XTT tests	1172:1180	XTT tests for ceramic extracts	1172:1201	The positive outcome of XTT tests for ceramic extracts demonstrated that all investigated cement-type composites may be considered cytocompatible according to ISO 10993-5 standard.
28681217	4	74	theme	components	593:602	arg1	effect					575:580	the effect	571:580	the effect of several components (calcite, hydroxyapatite doped with Mg2+, CO32- or Ag+ ions, alginate, chitosan and methylcellulose) on the physicochemical and biological properties of α-TCP-based bone cements	571:780	In our study we compared the effect of several components (calcite, hydroxyapatite doped with Mg2+, CO32- or Ag+ ions, alginate, chitosan and methylcellulose) on the physicochemical and biological properties of α-TCP-based bone cements.
28681217	2	75	theme	bone	367:370	arg1	fillers					372:378	the effective bone fillers	353:378	the effective bone fillers	353:378	Recently a great deal of attention has been focused on calcium phosphate cements (CPCs) as the effective bone fillers.
28681217	6	76	theme	activity	997:1004	arg1	test					1006:1009	metabolic activity test	987:1009	metabolic activity test	987:1009	A number of in vitro laboratory methods, including ICP-OES, metabolic activity test, time-lapse microscopic observation and SEM observations were performed in order to assess biocompatibility of the studied biomaterials.
26341978	0	0	theme	Lepidium	68:75	arg1	mucilage					56:63	the mucilage	52:63	the mucilage of Lepidium	52:75	Differentiation of the seed coat and composition of the mucilage of Lepidium perfoliatum L.: a desert annual with typical myxospermy.
26341978	8	1	theme	possible	1460:1467	arg1	role					1480:1483	the possible ecological role	1456:1483	the possible ecological role of mucilage in L. perfoliatum	1456:1513	These results suggest that the possible ecological role of mucilage in L. perfoliatum is in the adaptation to habitats with well-watered and water-logged conditions, rather than water stress.
26341978	6	2	theme	immunohistochemical	981:999	arg1	staining					1001:1008	immunohistochemical staining	981:1008	immunohistochemical staining combined with the enzymatic digestion and infrared spectrum analysis of the mucilage	981:1093	Thirdly, immunohistochemical staining combined with the enzymatic digestion and infrared spectrum analysis of the mucilage indicated that, while one important component of the seed coat mucilage in L. perfoliatum was pectin, it also contained β-1,3-d-glucan and xyloglucan.
26341978	2	3	theme	mucilage	328:335	arg1	production					337:346	mucilage production	328:346	mucilage production	328:346	Fertilization can initiate differentiation of the seed coat epidermis into a specialized cell type with mucilage production.
26341978	7	4	from	seeds	1383:1387	arg1	conditions					1417:1426	abundant or excess water conditions	1392:1426	abundant or excess water conditions	1392:1426	Finally, seed germination showed that seeds with mucilage displayed significantly higher germination percentage than that of demucilaged seeds in abundant or excess water conditions.
26341978	3	5	theme	seed	484:487	arg1	germination					489:499	seed germination	484:499	seed germination	484:499	In the present study, comprehensive analyses were performed on the seed coat differentiation, mucilage production and composition, and seed germination in Lepidium perfoliatum (Brassicaceae), a desert annual with typical myxospermy in China.
26341978	6	6	from	mucilage	1158:1165	arg1	perfoliatum					1173:1183	L. perfoliatum	1170:1183	L. perfoliatum	1170:1183	Thirdly, immunohistochemical staining combined with the enzymatic digestion and infrared spectrum analysis of the mucilage indicated that, while one important component of the seed coat mucilage in L. perfoliatum was pectin, it also contained β-1,3-d-glucan and xyloglucan.
26341978	3	7	with	annual	550:555	arg1	myxospermy					570:579	typical myxospermy	562:579	typical myxospermy in China	562:588	In the present study, comprehensive analyses were performed on the seed coat differentiation, mucilage production and composition, and seed germination in Lepidium perfoliatum (Brassicaceae), a desert annual with typical myxospermy in China.
26341978	6	8	theme	coat	1153:1156	arg1	mucilage					1158:1165	the seed coat mucilage	1144:1165	the seed coat mucilage in L. perfoliatum	1144:1183	Thirdly, immunohistochemical staining combined with the enzymatic digestion and infrared spectrum analysis of the mucilage indicated that, while one important component of the seed coat mucilage in L. perfoliatum was pectin, it also contained β-1,3-d-glucan and xyloglucan.
26341978	5	9	theme	material	911:918	arg1	production					875:884	production	875:884	production	875:884	Secondly, the inner tangential wall and two radial walls of the subepidermal cells were apparently thickened by production of a secondary cell wall material, which resulted in a 'typical' palisade appearance.
26341978	5	10	theme	wall	906:909	arg1	material					911:918	a secondary cell wall material	889:918	a secondary cell wall material	889:918	Secondly, the inner tangential wall and two radial walls of the subepidermal cells were apparently thickened by production of a secondary cell wall material, which resulted in a 'typical' palisade appearance.
26341978	7	11	theme	demucilaged	1371:1381	arg1	seeds					1383:1387	demucilaged seeds	1371:1387	demucilaged seeds in abundant or excess water conditions	1371:1426	Finally, seed germination showed that seeds with mucilage displayed significantly higher germination percentage than that of demucilaged seeds in abundant or excess water conditions.
26341978	7	12	theme	excess	1404:1409	arg1	conditions					1417:1426	abundant or excess water conditions	1392:1426	abundant or excess water conditions	1392:1426	Finally, seed germination showed that seeds with mucilage displayed significantly higher germination percentage than that of demucilaged seeds in abundant or excess water conditions.
26341978	4	13	theme	tangential	666:675	arg1	wall					677:680	the outer tangential wall	656:680	the outer tangential wall	656:680	First, results indicated that mucilage was secreted uniformly at the outer tangential wall, resulting in compression of the cytoplasm to the bottom of the epidermal cells.
26341978	6	14	contain	contained	1205:1213	arg1	it					1197:1198	it	1197:1198	it	1197:1198	Thirdly, immunohistochemical staining combined with the enzymatic digestion and infrared spectrum analysis of the mucilage indicated that, while one important component of the seed coat mucilage in L. perfoliatum was pectin, it also contained β-1,3-d-glucan and xyloglucan.
26341978	6	14	contain	contained	1205:1213	arg2	xyloglucan					1234:1243	xyloglucan	1234:1243	xyloglucan	1234:1243	Thirdly, immunohistochemical staining combined with the enzymatic digestion and infrared spectrum analysis of the mucilage indicated that, while one important component of the seed coat mucilage in L. perfoliatum was pectin, it also contained β-1,3-d-glucan and xyloglucan.
26341978	6	14	contain	contained	1205:1213	arg2	β-1,3-d-glucan					1215:1228	β-1,3-d-glucan	1215:1228	β-1,3-d-glucan	1215:1228	Thirdly, immunohistochemical staining combined with the enzymatic digestion and infrared spectrum analysis of the mucilage indicated that, while one important component of the seed coat mucilage in L. perfoliatum was pectin, it also contained β-1,3-d-glucan and xyloglucan.
26341978	8	15	theme	water	1607:1611	arg1	stress					1613:1618	water stress	1607:1618	water stress	1607:1618	These results suggest that the possible ecological role of mucilage in L. perfoliatum is in the adaptation to habitats with well-watered and water-logged conditions, rather than water stress.
26341978	4	16	theme	outer	660:664	arg1	wall					677:680	the outer tangential wall	656:680	the outer tangential wall	656:680	First, results indicated that mucilage was secreted uniformly at the outer tangential wall, resulting in compression of the cytoplasm to the bottom of the epidermal cells.
26341978	8	17	theme	mucilage	1488:1495	arg1	role					1480:1483	the possible ecological role	1456:1483	the possible ecological role of mucilage in L. perfoliatum	1456:1513	These results suggest that the possible ecological role of mucilage in L. perfoliatum is in the adaptation to habitats with well-watered and water-logged conditions, rather than water stress.
26341978	5	18	theme	secondary	891:899	arg1	material					911:918	a secondary cell wall material	889:918	a secondary cell wall material	889:918	Secondly, the inner tangential wall and two radial walls of the subepidermal cells were apparently thickened by production of a secondary cell wall material, which resulted in a 'typical' palisade appearance.
26341978	6	19	theme	important	1121:1129	arg1	component					1131:1139	one important component	1117:1139	one important component of the seed coat mucilage in L. perfoliatum	1117:1183	Thirdly, immunohistochemical staining combined with the enzymatic digestion and infrared spectrum analysis of the mucilage indicated that, while one important component of the seed coat mucilage in L. perfoliatum was pectin, it also contained β-1,3-d-glucan and xyloglucan.
26341978	3	20	theme	Lepidium	504:511	arg1	annual					550:555	a desert annual	541:555	a desert annual with typical myxospermy in China	541:588	In the present study, comprehensive analyses were performed on the seed coat differentiation, mucilage production and composition, and seed germination in Lepidium perfoliatum (Brassicaceae), a desert annual with typical myxospermy in China.
26341978	3	20	theme	Lepidium	504:511	arg1	Brassicaceae					526:537	Brassicaceae	526:537	Brassicaceae	526:537	In the present study, comprehensive analyses were performed on the seed coat differentiation, mucilage production and composition, and seed germination in Lepidium perfoliatum (Brassicaceae), a desert annual with typical myxospermy in China.
26341978	3	20	theme	Lepidium	504:511	arg1	perfoliatum					513:523	Lepidium perfoliatum	504:523	Lepidium perfoliatum (Brassicaceae)	504:538	In the present study, comprehensive analyses were performed on the seed coat differentiation, mucilage production and composition, and seed germination in Lepidium perfoliatum (Brassicaceae), a desert annual with typical myxospermy in China.
26341978	5	21	theme	radial	807:812	arg1	walls					814:818	two radial walls	803:818	two radial walls of the subepidermal cells	803:844	Secondly, the inner tangential wall and two radial walls of the subepidermal cells were apparently thickened by production of a secondary cell wall material, which resulted in a 'typical' palisade appearance.
26341978	7	22	theme	water	1411:1415	arg1	conditions					1417:1426	abundant or excess water conditions	1392:1426	abundant or excess water conditions	1392:1426	Finally, seed germination showed that seeds with mucilage displayed significantly higher germination percentage than that of demucilaged seeds in abundant or excess water conditions.
26341978	7	23	theme	seed	1255:1258	arg1	germination					1260:1270	seed germination	1255:1270	seed germination	1255:1270	Finally, seed germination showed that seeds with mucilage displayed significantly higher germination percentage than that of demucilaged seeds in abundant or excess water conditions.
26341978	7	24	theme	abundant	1392:1399	arg1	conditions					1417:1426	abundant or excess water conditions	1392:1426	abundant or excess water conditions	1392:1426	Finally, seed germination showed that seeds with mucilage displayed significantly higher germination percentage than that of demucilaged seeds in abundant or excess water conditions.
26341978	1	25	theme	important	151:159	arg1	feature					161:167	an important feature	148:167	an important feature in seeds of many plant species grown in desert region	148:221	Myxospermy is an important feature in seeds of many plant species grown in desert region.
26341978	1	25	theme	important	151:159	arg1	Myxospermy					134:143	Myxospermy	134:143	Myxospermy	134:143	Myxospermy is an important feature in seeds of many plant species grown in desert region.
26341978	3	26	theme	present	356:362	arg1	study					364:368	the present study	352:368	the present study	352:368	In the present study, comprehensive analyses were performed on the seed coat differentiation, mucilage production and composition, and seed germination in Lepidium perfoliatum (Brassicaceae), a desert annual with typical myxospermy in China.
26341978	6	27	theme	enzymatic	1028:1036	arg1	digestion					1038:1046	the enzymatic digestion	1024:1046	the enzymatic digestion	1024:1046	Thirdly, immunohistochemical staining combined with the enzymatic digestion and infrared spectrum analysis of the mucilage indicated that, while one important component of the seed coat mucilage in L. perfoliatum was pectin, it also contained β-1,3-d-glucan and xyloglucan.
26341978	5	28	theme	cell	901:904	arg1	material					911:918	a secondary cell wall material	889:918	a secondary cell wall material	889:918	Secondly, the inner tangential wall and two radial walls of the subepidermal cells were apparently thickened by production of a secondary cell wall material, which resulted in a 'typical' palisade appearance.
26341978	6	29	from	component	1131:1139	arg1	perfoliatum					1173:1183	L. perfoliatum	1170:1183	L. perfoliatum	1170:1183	Thirdly, immunohistochemical staining combined with the enzymatic digestion and infrared spectrum analysis of the mucilage indicated that, while one important component of the seed coat mucilage in L. perfoliatum was pectin, it also contained β-1,3-d-glucan and xyloglucan.
26341978	8	30	from	role	1480:1483	arg1	perfoliatum					1503:1513	L. perfoliatum	1500:1513	L. perfoliatum	1500:1513	These results suggest that the possible ecological role of mucilage in L. perfoliatum is in the adaptation to habitats with well-watered and water-logged conditions, rather than water stress.
26341978	2	31	theme	epidermis	284:292	arg1	differentiation					251:265	differentiation	251:265	differentiation of the seed coat epidermis into a specialized cell type with mucilage production	251:346	Fertilization can initiate differentiation of the seed coat epidermis into a specialized cell type with mucilage production.
26341978	0	32	theme	seed	23:26	arg1	coat					28:31	the seed coat	19:31	the seed coat	19:31	Differentiation of the seed coat and composition of the mucilage of Lepidium perfoliatum L.: a desert annual with typical myxospermy.
26341978	8	33	with	habitats	1539:1546	arg1	conditions					1583:1592	well-watered and water-logged conditions	1553:1592	well-watered and water-logged conditions	1553:1592	These results suggest that the possible ecological role of mucilage in L. perfoliatum is in the adaptation to habitats with well-watered and water-logged conditions, rather than water stress.
26341978	8	33	with	habitats	1539:1546	arg1	stress					1613:1618	water stress	1607:1618	water stress	1607:1618	These results suggest that the possible ecological role of mucilage in L. perfoliatum is in the adaptation to habitats with well-watered and water-logged conditions, rather than water stress.
26341978	6	34	from	perfoliatum	1173:1183	arg1	component					1131:1139	one important component	1117:1139	one important component of the seed coat mucilage in L. perfoliatum	1117:1183	Thirdly, immunohistochemical staining combined with the enzymatic digestion and infrared spectrum analysis of the mucilage indicated that, while one important component of the seed coat mucilage in L. perfoliatum was pectin, it also contained β-1,3-d-glucan and xyloglucan.
26341978	2	35	theme	coat	279:282	arg1	epidermis					284:292	the seed coat epidermis	270:292	the seed coat epidermis	270:292	Fertilization can initiate differentiation of the seed coat epidermis into a specialized cell type with mucilage production.
26341978	5	36	theme	tangential	783:792	arg1	wall					794:797	the inner tangential wall	773:797	the inner tangential wall	773:797	Secondly, the inner tangential wall and two radial walls of the subepidermal cells were apparently thickened by production of a secondary cell wall material, which resulted in a 'typical' palisade appearance.
26341978	3	37	theme	desert	543:548	arg1	annual					550:555	a desert annual	541:555	a desert annual with typical myxospermy in China	541:588	In the present study, comprehensive analyses were performed on the seed coat differentiation, mucilage production and composition, and seed germination in Lepidium perfoliatum (Brassicaceae), a desert annual with typical myxospermy in China.
26341978	3	37	theme	desert	543:548	arg1	perfoliatum					513:523	Lepidium perfoliatum	504:523	Lepidium perfoliatum (Brassicaceae)	504:538	In the present study, comprehensive analyses were performed on the seed coat differentiation, mucilage production and composition, and seed germination in Lepidium perfoliatum (Brassicaceae), a desert annual with typical myxospermy in China.
26341978	0	38	theme	desert	95:100	arg1	annual					102:107	a desert annual	93:107	Differentiation of the seed coat and composition of the mucilage of Lepidium perfoliatum L.: a desert annual with typical myxospermy.	0:132	Differentiation of the seed coat and composition of the mucilage of Lepidium perfoliatum L.: a desert annual with typical myxospermy.
26341978	2	39	theme	seed	274:277	arg1	epidermis					284:292	the seed coat epidermis	270:292	the seed coat epidermis	270:292	Fertilization can initiate differentiation of the seed coat epidermis into a specialized cell type with mucilage production.
26341978	3	40	theme	mucilage	443:450	arg1	production					452:461	mucilage production	443:461	mucilage production	443:461	In the present study, comprehensive analyses were performed on the seed coat differentiation, mucilage production and composition, and seed germination in Lepidium perfoliatum (Brassicaceae), a desert annual with typical myxospermy in China.
26341978	5	41	theme	typical	942:948	arg1	appearance					960:969	a 'typical' palisade appearance	939:969	a 'typical' palisade appearance	939:969	Secondly, the inner tangential wall and two radial walls of the subepidermal cells were apparently thickened by production of a secondary cell wall material, which resulted in a 'typical' palisade appearance.
26341978	0	42	theme	typical	114:120	arg1	myxospermy					122:131	typical myxospermy	114:131	typical myxospermy	114:131	Differentiation of the seed coat and composition of the mucilage of Lepidium perfoliatum L.: a desert annual with typical myxospermy.
26341978	3	43	theme	coat	421:424	arg1	differentiation					426:440	the seed coat differentiation	412:440	the seed coat differentiation	412:440	In the present study, comprehensive analyses were performed on the seed coat differentiation, mucilage production and composition, and seed germination in Lepidium perfoliatum (Brassicaceae), a desert annual with typical myxospermy in China.
26341978	3	44	theme	typical	562:568	arg1	myxospermy					570:579	typical myxospermy	562:579	typical myxospermy in China	562:588	In the present study, comprehensive analyses were performed on the seed coat differentiation, mucilage production and composition, and seed germination in Lepidium perfoliatum (Brassicaceae), a desert annual with typical myxospermy in China.
26341978	3	45	from	myxospermy	570:579	arg1	China					584:588	China	584:588	China	584:588	In the present study, comprehensive analyses were performed on the seed coat differentiation, mucilage production and composition, and seed germination in Lepidium perfoliatum (Brassicaceae), a desert annual with typical myxospermy in China.
26341978	1	46	theme	many	181:184	arg1	species					192:198	many plant species	181:198	many plant species grown in desert region	181:221	Myxospermy is an important feature in seeds of many plant species grown in desert region.
26341978	0	47	theme	coat	28:31	arg1	Differentiation					0:14	Differentiation	0:14	Differentiation of the seed coat	0:31	Differentiation of the seed coat and composition of the mucilage of Lepidium perfoliatum L.: a desert annual with typical myxospermy.
26341978	0	47	theme	coat	28:31	arg1	composition					37:47	composition	37:47	composition	37:47	Differentiation of the seed coat and composition of the mucilage of Lepidium perfoliatum L.: a desert annual with typical myxospermy.
26341978	2	48	with	type	318:321	arg1	production					337:346	mucilage production	328:346	mucilage production	328:346	Fertilization can initiate differentiation of the seed coat epidermis into a specialized cell type with mucilage production.
26341978	5	49	theme	palisade	951:958	arg1	appearance					960:969	a 'typical' palisade appearance	939:969	a 'typical' palisade appearance	939:969	Secondly, the inner tangential wall and two radial walls of the subepidermal cells were apparently thickened by production of a secondary cell wall material, which resulted in a 'typical' palisade appearance.
26341978	1	50	theme	plant	186:190	arg1	species					192:198	many plant species	181:198	many plant species grown in desert region	181:221	Myxospermy is an important feature in seeds of many plant species grown in desert region.
26341978	7	51	theme	higher	1328:1333	arg1	percentage					1347:1356	significantly higher germination percentage	1314:1356	significantly higher germination percentage	1314:1356	Finally, seed germination showed that seeds with mucilage displayed significantly higher germination percentage than that of demucilaged seeds in abundant or excess water conditions.
26341978	6	52	theme	spectrum	1061:1068	arg1	analysis					1070:1077	infrared spectrum analysis	1052:1077	infrared spectrum analysis	1052:1077	Thirdly, immunohistochemical staining combined with the enzymatic digestion and infrared spectrum analysis of the mucilage indicated that, while one important component of the seed coat mucilage in L. perfoliatum was pectin, it also contained β-1,3-d-glucan and xyloglucan.
26341978	6	53	theme	mucilage	1086:1093	arg1	digestion					1038:1046	the enzymatic digestion	1024:1046	the enzymatic digestion	1024:1046	Thirdly, immunohistochemical staining combined with the enzymatic digestion and infrared spectrum analysis of the mucilage indicated that, while one important component of the seed coat mucilage in L. perfoliatum was pectin, it also contained β-1,3-d-glucan and xyloglucan.
26341978	6	53	theme	mucilage	1086:1093	arg1	analysis					1070:1077	infrared spectrum analysis	1052:1077	infrared spectrum analysis	1052:1077	Thirdly, immunohistochemical staining combined with the enzymatic digestion and infrared spectrum analysis of the mucilage indicated that, while one important component of the seed coat mucilage in L. perfoliatum was pectin, it also contained β-1,3-d-glucan and xyloglucan.
26341978	3	54	theme	comprehensive	371:383	arg1	analyses					385:392	comprehensive analyses	371:392	comprehensive analyses	371:392	In the present study, comprehensive analyses were performed on the seed coat differentiation, mucilage production and composition, and seed germination in Lepidium perfoliatum (Brassicaceae), a desert annual with typical myxospermy in China.
26341978	8	55	theme	well-watered	1553:1564	arg1	conditions					1583:1592	well-watered and water-logged conditions	1553:1592	well-watered and water-logged conditions	1553:1592	These results suggest that the possible ecological role of mucilage in L. perfoliatum is in the adaptation to habitats with well-watered and water-logged conditions, rather than water stress.
26341978	7	56	theme	germination	1335:1345	arg1	percentage					1347:1356	significantly higher germination percentage	1314:1356	significantly higher germination percentage	1314:1356	Finally, seed germination showed that seeds with mucilage displayed significantly higher germination percentage than that of demucilaged seeds in abundant or excess water conditions.
26341978	2	57	theme	cell	313:316	arg1	type					318:321	a specialized cell type	299:321	a specialized cell type with mucilage production	299:346	Fertilization can initiate differentiation of the seed coat epidermis into a specialized cell type with mucilage production.
26341978	4	58	theme	cells	756:760	arg1	bottom					732:737	the bottom	728:737	the bottom of the epidermal cells	728:760	First, results indicated that mucilage was secreted uniformly at the outer tangential wall, resulting in compression of the cytoplasm to the bottom of the epidermal cells.
26341978	6	59	theme	mucilage	1158:1165	arg1	component					1131:1139	one important component	1117:1139	one important component of the seed coat mucilage in L. perfoliatum	1117:1183	Thirdly, immunohistochemical staining combined with the enzymatic digestion and infrared spectrum analysis of the mucilage indicated that, while one important component of the seed coat mucilage in L. perfoliatum was pectin, it also contained β-1,3-d-glucan and xyloglucan.
26341978	4	60	theme	epidermal	746:754	arg1	cells					756:760	the epidermal cells	742:760	the epidermal cells	742:760	First, results indicated that mucilage was secreted uniformly at the outer tangential wall, resulting in compression of the cytoplasm to the bottom of the epidermal cells.
26341978	2	61	theme	specialized	301:311	arg1	type					318:321	a specialized cell type	299:321	a specialized cell type with mucilage production	299:346	Fertilization can initiate differentiation of the seed coat epidermis into a specialized cell type with mucilage production.
26341978	4	62	theme	cytoplasm	715:723	arg1	compression					696:706	compression	696:706	compression of the cytoplasm to the bottom of the epidermal cells	696:760	First, results indicated that mucilage was secreted uniformly at the outer tangential wall, resulting in compression of the cytoplasm to the bottom of the epidermal cells.
26341978	1	63	theme	species	192:198	arg1	seeds					172:176	seeds	172:176	seeds of many plant species grown in desert region	172:221	Myxospermy is an important feature in seeds of many plant species grown in desert region.
26341978	7	64	with	seeds	1284:1288	arg1	mucilage					1295:1302	mucilage	1295:1302	mucilage	1295:1302	Finally, seed germination showed that seeds with mucilage displayed significantly higher germination percentage than that of demucilaged seeds in abundant or excess water conditions.
26341978	5	65	theme	inner	777:781	arg1	wall					794:797	the inner tangential wall	773:797	the inner tangential wall	773:797	Secondly, the inner tangential wall and two radial walls of the subepidermal cells were apparently thickened by production of a secondary cell wall material, which resulted in a 'typical' palisade appearance.
26341978	1	66	from	feature	161:167	arg1	seeds					172:176	seeds	172:176	seeds of many plant species grown in desert region	172:221	Myxospermy is an important feature in seeds of many plant species grown in desert region.
26341978	6	67	theme	infrared	1052:1059	arg1	analysis					1070:1077	infrared spectrum analysis	1052:1077	infrared spectrum analysis	1052:1077	Thirdly, immunohistochemical staining combined with the enzymatic digestion and infrared spectrum analysis of the mucilage indicated that, while one important component of the seed coat mucilage in L. perfoliatum was pectin, it also contained β-1,3-d-glucan and xyloglucan.
26341978	0	68	with	annual	102:107	arg1	myxospermy					122:131	typical myxospermy	114:131	typical myxospermy	114:131	Differentiation of the seed coat and composition of the mucilage of Lepidium perfoliatum L.: a desert annual with typical myxospermy.
26341978	8	69	theme	water-logged	1570:1581	arg1	conditions					1583:1592	well-watered and water-logged conditions	1553:1592	well-watered and water-logged conditions	1553:1592	These results suggest that the possible ecological role of mucilage in L. perfoliatum is in the adaptation to habitats with well-watered and water-logged conditions, rather than water stress.
26341978	0	70	theme	mucilage	56:63	arg1	Differentiation					0:14	Differentiation	0:14	Differentiation of the seed coat	0:31	Differentiation of the seed coat and composition of the mucilage of Lepidium perfoliatum L.: a desert annual with typical myxospermy.
26341978	0	70	theme	mucilage	56:63	arg1	composition					37:47	composition	37:47	composition	37:47	Differentiation of the seed coat and composition of the mucilage of Lepidium perfoliatum L.: a desert annual with typical myxospermy.
26341978	6	71	theme	seed	1148:1151	arg1	mucilage					1158:1165	the seed coat mucilage	1144:1165	the seed coat mucilage in L. perfoliatum	1144:1183	Thirdly, immunohistochemical staining combined with the enzymatic digestion and infrared spectrum analysis of the mucilage indicated that, while one important component of the seed coat mucilage in L. perfoliatum was pectin, it also contained β-1,3-d-glucan and xyloglucan.
26341978	5	72	theme	subepidermal	827:838	arg1	cells					840:844	the subepidermal cells	823:844	the subepidermal cells	823:844	Secondly, the inner tangential wall and two radial walls of the subepidermal cells were apparently thickened by production of a secondary cell wall material, which resulted in a 'typical' palisade appearance.
26341978	3	73	theme	seed	416:419	arg1	differentiation					426:440	the seed coat differentiation	412:440	the seed coat differentiation	412:440	In the present study, comprehensive analyses were performed on the seed coat differentiation, mucilage production and composition, and seed germination in Lepidium perfoliatum (Brassicaceae), a desert annual with typical myxospermy in China.
26341978	8	74	theme	ecological	1469:1478	arg1	role					1480:1483	the possible ecological role	1456:1483	the possible ecological role of mucilage in L. perfoliatum	1456:1513	These results suggest that the possible ecological role of mucilage in L. perfoliatum is in the adaptation to habitats with well-watered and water-logged conditions, rather than water stress.
26341978	0	75	dep	Differentiation	0:14	arg1	annual					102:107	a desert annual	93:107	Differentiation of the seed coat and composition of the mucilage of Lepidium perfoliatum L.: a desert annual with typical myxospermy.	0:132	Differentiation of the seed coat and composition of the mucilage of Lepidium perfoliatum L.: a desert annual with typical myxospermy.
26341978	5	76	theme	cells	840:844	arg1	walls					814:818	two radial walls	803:818	two radial walls of the subepidermal cells	803:844	Secondly, the inner tangential wall and two radial walls of the subepidermal cells were apparently thickened by production of a secondary cell wall material, which resulted in a 'typical' palisade appearance.
26341978	5	76	theme	cells	840:844	arg1	wall					794:797	the inner tangential wall	773:797	the inner tangential wall	773:797	Secondly, the inner tangential wall and two radial walls of the subepidermal cells were apparently thickened by production of a secondary cell wall material, which resulted in a 'typical' palisade appearance.
26341978	1	77	theme	desert	209:214	arg1	region					216:221	desert region	209:221	desert region	209:221	Myxospermy is an important feature in seeds of many plant species grown in desert region.
25863346	0	0	theme	single-chain	76:87	arg1	analogs					100:106	single-chain bovine FSH analogs	76:106	single-chain bovine FSH analogs	76:106	Modulation of the steroidogenic related activity according to the design of single-chain bovine FSH analogs.
25863346	4	1	theme	secretion	829:837	arg1	efficiency					839:848	the secretion efficiency	825:848	the secretion efficiency of these variants from the transfected cells	825:893	The data suggested that the secretion efficiency of these variants from the transfected cells was unaffected by the presence or absence of the CTP linker, N-glycans were attached to the analogs and the hCGβ-CTP domain in the FSHβCTPα variant was O-glycosylated.
25863346	5	2	theme	granulosa	1085:1093	arg1	bioassay					1100:1107	a rat immortalized granulosa cell bioassay	1066:1107	a rat immortalized granulosa cell bioassay	1066:1107	In a rat immortalized granulosa cell bioassay the potency of the three variants towards progesterone secretion varied.
25863346	9	3	theme	new	1677:1679	arg1	factor/s					1692:1699	new regulatory factor/s	1677:1699	new regulatory factor/s involved in the pleiotropic FSH activity	1677:1740	A systematic analysis of receptor activation with ligands carrying structural differences may identify new regulatory factor/s involved in the pleiotropic FSH activity.
25863346	2	4	theme	related	330:336	arg1	activity					338:345	the steroidogenic related activity	312:345	the steroidogenic related activity of SC FSH analogs carrying structural differences	312:395	The major aim of the current study was to examine the steroidogenic related activity of SC FSH analogs carrying structural differences.
25863346	5	5	theme	cell	1095:1098	arg1	bioassay					1100:1107	a rat immortalized granulosa cell bioassay	1066:1107	a rat immortalized granulosa cell bioassay	1066:1107	In a rat immortalized granulosa cell bioassay the potency of the three variants towards progesterone secretion varied.
25863346	1	6	theme	domestic	244:251	arg1	animals					253:259	domestic animals	244:259	domestic animals	244:259	Single-chain (SC) gonadotropins have been genetically engineered to increase the repertoire of analogs for potential use in humans and domestic animals.
25863346	0	7	theme	FSH	96:98	arg1	analogs					100:106	single-chain bovine FSH analogs	76:106	single-chain bovine FSH analogs	76:106	Modulation of the steroidogenic related activity according to the design of single-chain bovine FSH analogs.
25863346	9	8	theme	systematic	1576:1585	arg1	analysis					1587:1594	A systematic analysis	1574:1594	A systematic analysis of receptor activation with ligands carrying structural differences	1574:1662	A systematic analysis of receptor activation with ligands carrying structural differences may identify new regulatory factor/s involved in the pleiotropic FSH activity.
25863346	4	9	from	domain	1012:1017	arg1	variant					1035:1041	the FSHβCTPα variant	1022:1041	the FSHβCTPα variant	1022:1041	The data suggested that the secretion efficiency of these variants from the transfected cells was unaffected by the presence or absence of the CTP linker, N-glycans were attached to the analogs and the hCGβ-CTP domain in the FSHβCTPα variant was O-glycosylated.
25863346	9	10	theme	FSH	1729:1731	arg1	activity					1733:1740	the pleiotropic FSH activity	1713:1740	the pleiotropic FSH activity	1713:1740	A systematic analysis of receptor activation with ligands carrying structural differences may identify new regulatory factor/s involved in the pleiotropic FSH activity.
25863346	6	11	theme	Cyp17	1275:1279	arg1	transcripts					1317:1327	StAR, Cyp11a (P450scc), Cyp17 (P450c17) and Cyp19 (P450aromatase) transcripts	1251:1327	StAR, Cyp11a (P450scc), Cyp17 (P450c17) and Cyp19 (P450aromatase) transcripts	1251:1327	In immature mice, the analogs increased the ovary weight and induced StAR, Cyp11a (P450scc), Cyp17 (P450c17) and Cyp19 (P450aromatase) transcripts.
25863346	0	12	theme	bovine	89:94	arg1	analogs					100:106	single-chain bovine FSH analogs	76:106	single-chain bovine FSH analogs	76:106	Modulation of the steroidogenic related activity according to the design of single-chain bovine FSH analogs.
25863346	3	13	theme	human	629:633	arg1	hCG					655:657	hCG	655:657	hCG	655:657	To address this issue, we designed and expressed three SC bovine FSH analogs in CHO cells: (i) FSHβα in which the tethered subunit domains are linked in tandem; (ii) FSHβCTPα that contains the carboxy terminal peptide (CTP) of the human choriogonadotropin (hCG) β subunit as a spacer, and (iii) FSHβboCTPα in which the linker is derived from a CTP-like sequence (boCTP) decoded from the bovine LHβ DNA.
25863346	3	13	theme	human	629:633	arg1	choriogonadotropin					635:652	the human choriogonadotropin	625:652	the human choriogonadotropin (hCG) β subunit	625:668	To address this issue, we designed and expressed three SC bovine FSH analogs in CHO cells: (i) FSHβα in which the tethered subunit domains are linked in tandem; (ii) FSHβCTPα that contains the carboxy terminal peptide (CTP) of the human choriogonadotropin (hCG) β subunit as a spacer, and (iii) FSHβboCTPα in which the linker is derived from a CTP-like sequence (boCTP) decoded from the bovine LHβ DNA.
25863346	3	14	dep	FSHβα	493:497	arg1	i					490:490	i	490:490	i	490:490	To address this issue, we designed and expressed three SC bovine FSH analogs in CHO cells: (i) FSHβα in which the tethered subunit domains are linked in tandem; (ii) FSHβCTPα that contains the carboxy terminal peptide (CTP) of the human choriogonadotropin (hCG) β subunit as a spacer, and (iii) FSHβboCTPα in which the linker is derived from a CTP-like sequence (boCTP) decoded from the bovine LHβ DNA.
25863346	2	15	theme	steroidogenic	316:328	arg1	activity					338:345	the steroidogenic related activity	312:345	the steroidogenic related activity of SC FSH analogs carrying structural differences	312:395	The major aim of the current study was to examine the steroidogenic related activity of SC FSH analogs carrying structural differences.
25863346	5	16	theme	immortalized	1072:1083	arg1	bioassay					1100:1107	a rat immortalized granulosa cell bioassay	1066:1107	a rat immortalized granulosa cell bioassay	1066:1107	In a rat immortalized granulosa cell bioassay the potency of the three variants towards progesterone secretion varied.
25863346	2	17	theme	major	266:270	arg1	aim					272:274	The major aim	262:274	The major aim of the current study	262:295	The major aim of the current study was to examine the steroidogenic related activity of SC FSH analogs carrying structural differences.
25863346	8	18	theme	analog	1516:1521	arg1	design					1498:1503	the design	1494:1503	the design of the FSH analog	1494:1521	Collectively, these data suggest that the design of the FSH analog can modulate the bioactivity in vitro and in vivo.
25863346	2	19	theme	structural	374:383	arg1	differences					385:395	structural differences	374:395	structural differences	374:395	The major aim of the current study was to examine the steroidogenic related activity of SC FSH analogs carrying structural differences.
25863346	0	20	theme	analogs	100:106	arg1	design					66:71	the design	62:71	the design of single-chain bovine FSH analogs	62:106	Modulation of the steroidogenic related activity according to the design of single-chain bovine FSH analogs.
25863346	6	21	theme	StAR	1251:1254	arg1	transcripts					1317:1327	StAR, Cyp11a (P450scc), Cyp17 (P450c17) and Cyp19 (P450aromatase) transcripts	1251:1327	StAR, Cyp11a (P450scc), Cyp17 (P450c17) and Cyp19 (P450aromatase) transcripts	1251:1327	In immature mice, the analogs increased the ovary weight and induced StAR, Cyp11a (P450scc), Cyp17 (P450c17) and Cyp19 (P450aromatase) transcripts.
25863346	5	22	theme	variants	1134:1141	arg1	potency					1113:1119	the potency	1109:1119	the potency of the three variants towards progesterone secretion	1109:1172	In a rat immortalized granulosa cell bioassay the potency of the three variants towards progesterone secretion varied.
25863346	3	23	dep	analogs	467:473	arg1	FSHβCTPα					564:571	(ii) FSHβCTPα	559:571	three SC bovine FSH analogs in CHO cells: (i) FSHβα in which the tethered subunit domains are linked in tandem; (ii) FSHβCTPα that contains the carboxy terminal peptide (CTP) of the human choriogonadotropin (hCG) β subunit as a spacer, and (iii) FSHβboCTPα in which the linker is derived from a CTP-like sequence (boCTP) decoded from the bovine LHβ DNA	447:798	To address this issue, we designed and expressed three SC bovine FSH analogs in CHO cells: (i) FSHβα in which the tethered subunit domains are linked in tandem; (ii) FSHβCTPα that contains the carboxy terminal peptide (CTP) of the human choriogonadotropin (hCG) β subunit as a spacer, and (iii) FSHβboCTPα in which the linker is derived from a CTP-like sequence (boCTP) decoded from the bovine LHβ DNA.
25863346	3	23	dep	analogs	467:473	arg1	FSHβα					493:497	(i) FSHβα	489:497	three SC bovine FSH analogs in CHO cells: (i) FSHβα in which the tethered subunit domains are linked in tandem; (ii) FSHβCTPα that contains the carboxy terminal peptide (CTP) of the human choriogonadotropin (hCG) β subunit as a spacer, and (iii) FSHβboCTPα in which the linker is derived from a CTP-like sequence (boCTP) decoded from the bovine LHβ DNA	447:798	To address this issue, we designed and expressed three SC bovine FSH analogs in CHO cells: (i) FSHβα in which the tethered subunit domains are linked in tandem; (ii) FSHβCTPα that contains the carboxy terminal peptide (CTP) of the human choriogonadotropin (hCG) β subunit as a spacer, and (iii) FSHβboCTPα in which the linker is derived from a CTP-like sequence (boCTP) decoded from the bovine LHβ DNA.
25863346	3	24	theme	FSH	463:465	arg1	analogs					467:473	three SC bovine FSH analogs	447:473	three SC bovine FSH analogs in CHO cells: (i) FSHβα in which the tethered subunit domains are linked in tandem; (ii) FSHβCTPα that contains the carboxy terminal peptide (CTP) of the human choriogonadotropin (hCG) β subunit as a spacer, and (iii) FSHβboCTPα in which the linker is derived from a CTP-like sequence (boCTP) decoded from the bovine LHβ DNA	447:798	To address this issue, we designed and expressed three SC bovine FSH analogs in CHO cells: (i) FSHβα in which the tethered subunit domains are linked in tandem; (ii) FSHβCTPα that contains the carboxy terminal peptide (CTP) of the human choriogonadotropin (hCG) β subunit as a spacer, and (iii) FSHβboCTPα in which the linker is derived from a CTP-like sequence (boCTP) decoded from the bovine LHβ DNA.
25863346	3	25	contain	contains	578:585	arg1	FSHβCTPα					564:571	(ii) FSHβCTPα	559:571	three SC bovine FSH analogs in CHO cells: (i) FSHβα in which the tethered subunit domains are linked in tandem; (ii) FSHβCTPα that contains the carboxy terminal peptide (CTP) of the human choriogonadotropin (hCG) β subunit as a spacer, and (iii) FSHβboCTPα in which the linker is derived from a CTP-like sequence (boCTP) decoded from the bovine LHβ DNA	447:798	To address this issue, we designed and expressed three SC bovine FSH analogs in CHO cells: (i) FSHβα in which the tethered subunit domains are linked in tandem; (ii) FSHβCTPα that contains the carboxy terminal peptide (CTP) of the human choriogonadotropin (hCG) β subunit as a spacer, and (iii) FSHβboCTPα in which the linker is derived from a CTP-like sequence (boCTP) decoded from the bovine LHβ DNA.
25863346	3	25	contain	contains	578:585	arg2	peptide					608:614	the carboxy terminal peptide	587:614	the carboxy terminal peptide (CTP) of the human choriogonadotropin (hCG) β subunit	587:668	To address this issue, we designed and expressed three SC bovine FSH analogs in CHO cells: (i) FSHβα in which the tethered subunit domains are linked in tandem; (ii) FSHβCTPα that contains the carboxy terminal peptide (CTP) of the human choriogonadotropin (hCG) β subunit as a spacer, and (iii) FSHβboCTPα in which the linker is derived from a CTP-like sequence (boCTP) decoded from the bovine LHβ DNA.
25863346	3	25	contain	contains	578:585	arg2	CTP					617:619	CTP	617:619	CTP	617:619	To address this issue, we designed and expressed three SC bovine FSH analogs in CHO cells: (i) FSHβα in which the tethered subunit domains are linked in tandem; (ii) FSHβCTPα that contains the carboxy terminal peptide (CTP) of the human choriogonadotropin (hCG) β subunit as a spacer, and (iii) FSHβboCTPα in which the linker is derived from a CTP-like sequence (boCTP) decoded from the bovine LHβ DNA.
25863346	3	25	contain	contains	578:585	arg2	spacer					675:680	a spacer	673:680	a spacer	673:680	To address this issue, we designed and expressed three SC bovine FSH analogs in CHO cells: (i) FSHβα in which the tethered subunit domains are linked in tandem; (ii) FSHβCTPα that contains the carboxy terminal peptide (CTP) of the human choriogonadotropin (hCG) β subunit as a spacer, and (iii) FSHβboCTPα in which the linker is derived from a CTP-like sequence (boCTP) decoded from the bovine LHβ DNA.
25863346	4	26	theme	FSHβCTPα	1026:1033	arg1	variant					1035:1041	the FSHβCTPα variant	1022:1041	the FSHβCTPα variant	1022:1041	The data suggested that the secretion efficiency of these variants from the transfected cells was unaffected by the presence or absence of the CTP linker, N-glycans were attached to the analogs and the hCGβ-CTP domain in the FSHβCTPα variant was O-glycosylated.
25863346	3	27	theme	choriogonadotropin	635:652	arg1	subunit					662:668	the human choriogonadotropin (hCG) β subunit	625:668	the human choriogonadotropin (hCG) β subunit	625:668	To address this issue, we designed and expressed three SC bovine FSH analogs in CHO cells: (i) FSHβα in which the tethered subunit domains are linked in tandem; (ii) FSHβCTPα that contains the carboxy terminal peptide (CTP) of the human choriogonadotropin (hCG) β subunit as a spacer, and (iii) FSHβboCTPα in which the linker is derived from a CTP-like sequence (boCTP) decoded from the bovine LHβ DNA.
25863346	2	28	theme	analogs	357:363	arg1	activity					338:345	the steroidogenic related activity	312:345	the steroidogenic related activity of SC FSH analogs carrying structural differences	312:395	The major aim of the current study was to examine the steroidogenic related activity of SC FSH analogs carrying structural differences.
25863346	8	29	theme	FSH	1512:1514	arg1	analog					1516:1521	the FSH analog	1508:1521	the FSH analog	1508:1521	Collectively, these data suggest that the design of the FSH analog can modulate the bioactivity in vitro and in vivo.
25863346	4	30	from	cells	889:893	arg1	efficiency					839:848	the secretion efficiency	825:848	the secretion efficiency of these variants from the transfected cells	825:893	The data suggested that the secretion efficiency of these variants from the transfected cells was unaffected by the presence or absence of the CTP linker, N-glycans were attached to the analogs and the hCGβ-CTP domain in the FSHβCTPα variant was O-glycosylated.
25863346	2	31	theme	study	291:295	arg1	aim					272:274	The major aim	262:274	The major aim of the current study	262:295	The major aim of the current study was to examine the steroidogenic related activity of SC FSH analogs carrying structural differences.
25863346	3	32	theme	bovine	785:790	arg1	DNA					796:798	the bovine LHβ DNA	781:798	the bovine LHβ DNA	781:798	To address this issue, we designed and expressed three SC bovine FSH analogs in CHO cells: (i) FSHβα in which the tethered subunit domains are linked in tandem; (ii) FSHβCTPα that contains the carboxy terminal peptide (CTP) of the human choriogonadotropin (hCG) β subunit as a spacer, and (iii) FSHβboCTPα in which the linker is derived from a CTP-like sequence (boCTP) decoded from the bovine LHβ DNA.
25863346	2	33	theme	FSH	353:355	arg1	analogs					357:363	SC FSH analogs	350:363	SC FSH analogs carrying structural differences	350:395	The major aim of the current study was to examine the steroidogenic related activity of SC FSH analogs carrying structural differences.
25863346	0	34	theme	steroidogenic	18:30	arg1	activity					40:47	the steroidogenic related activity	14:47	the steroidogenic related activity	14:47	Modulation of the steroidogenic related activity according to the design of single-chain bovine FSH analogs.
25863346	3	35	theme	β	660:660	arg1	subunit					662:668	the human choriogonadotropin (hCG) β subunit	625:668	the human choriogonadotropin (hCG) β subunit	625:668	To address this issue, we designed and expressed three SC bovine FSH analogs in CHO cells: (i) FSHβα in which the tethered subunit domains are linked in tandem; (ii) FSHβCTPα that contains the carboxy terminal peptide (CTP) of the human choriogonadotropin (hCG) β subunit as a spacer, and (iii) FSHβboCTPα in which the linker is derived from a CTP-like sequence (boCTP) decoded from the bovine LHβ DNA.
25863346	3	36	from	analogs	467:473	arg1	cells					482:486	CHO cells	478:486	CHO cells	478:486	To address this issue, we designed and expressed three SC bovine FSH analogs in CHO cells: (i) FSHβα in which the tethered subunit domains are linked in tandem; (ii) FSHβCTPα that contains the carboxy terminal peptide (CTP) of the human choriogonadotropin (hCG) β subunit as a spacer, and (iii) FSHβboCTPα in which the linker is derived from a CTP-like sequence (boCTP) decoded from the bovine LHβ DNA.
25863346	2	37	theme	current	283:289	arg1	study					291:295	the current study	279:295	the current study	279:295	The major aim of the current study was to examine the steroidogenic related activity of SC FSH analogs carrying structural differences.
25863346	3	38	theme	LHβ	792:794	arg1	DNA					796:798	the bovine LHβ DNA	781:798	the bovine LHβ DNA	781:798	To address this issue, we designed and expressed three SC bovine FSH analogs in CHO cells: (i) FSHβα in which the tethered subunit domains are linked in tandem; (ii) FSHβCTPα that contains the carboxy terminal peptide (CTP) of the human choriogonadotropin (hCG) β subunit as a spacer, and (iii) FSHβboCTPα in which the linker is derived from a CTP-like sequence (boCTP) decoded from the bovine LHβ DNA.
25863346	3	39	theme	tethered	512:519	arg1	domains					529:535	the tethered subunit domains	508:535	the tethered subunit domains	508:535	To address this issue, we designed and expressed three SC bovine FSH analogs in CHO cells: (i) FSHβα in which the tethered subunit domains are linked in tandem; (ii) FSHβCTPα that contains the carboxy terminal peptide (CTP) of the human choriogonadotropin (hCG) β subunit as a spacer, and (iii) FSHβboCTPα in which the linker is derived from a CTP-like sequence (boCTP) decoded from the bovine LHβ DNA.
25863346	9	40	with	activation	1608:1617	arg1	ligands					1624:1630	ligands	1624:1630	ligands carrying structural differences	1624:1662	A systematic analysis of receptor activation with ligands carrying structural differences may identify new regulatory factor/s involved in the pleiotropic FSH activity.
25863346	3	41	theme	CHO	478:480	arg1	cells					482:486	CHO cells	478:486	CHO cells	478:486	To address this issue, we designed and expressed three SC bovine FSH analogs in CHO cells: (i) FSHβα in which the tethered subunit domains are linked in tandem; (ii) FSHβCTPα that contains the carboxy terminal peptide (CTP) of the human choriogonadotropin (hCG) β subunit as a spacer, and (iii) FSHβboCTPα in which the linker is derived from a CTP-like sequence (boCTP) decoded from the bovine LHβ DNA.
25863346	4	42	theme	CTP	944:946	arg1	linker					948:953	the CTP linker	940:953	the CTP linker	940:953	The data suggested that the secretion efficiency of these variants from the transfected cells was unaffected by the presence or absence of the CTP linker, N-glycans were attached to the analogs and the hCGβ-CTP domain in the FSHβCTPα variant was O-glycosylated.
25863346	0	43	theme	activity	40:47	arg1	Modulation					0:9	Modulation	0:9	Modulation of the steroidogenic related activity	0:47	Modulation of the steroidogenic related activity according to the design of single-chain bovine FSH analogs.
25863346	4	44	attach	attached	971:978	arg2	N-glycans					956:964	N-glycans	956:964	N-glycans	956:964	The data suggested that the secretion efficiency of these variants from the transfected cells was unaffected by the presence or absence of the CTP linker, N-glycans were attached to the analogs and the hCGβ-CTP domain in the FSHβCTPα variant was O-glycosylated.
25863346	4	44	attach	attached	971:978	arg1	analogs					987:993	the analogs	983:993	the analogs	983:993	The data suggested that the secretion efficiency of these variants from the transfected cells was unaffected by the presence or absence of the CTP linker, N-glycans were attached to the analogs and the hCGβ-CTP domain in the FSHβCTPα variant was O-glycosylated.
25863346	9	45	theme	regulatory	1681:1690	arg1	factor/s					1692:1699	new regulatory factor/s	1677:1699	new regulatory factor/s involved in the pleiotropic FSH activity	1677:1740	A systematic analysis of receptor activation with ligands carrying structural differences may identify new regulatory factor/s involved in the pleiotropic FSH activity.
25863346	4	46	theme	linker	948:953	arg1	absence					929:935	absence	929:935	absence	929:935	The data suggested that the secretion efficiency of these variants from the transfected cells was unaffected by the presence or absence of the CTP linker, N-glycans were attached to the analogs and the hCGβ-CTP domain in the FSHβCTPα variant was O-glycosylated.
25863346	4	46	theme	linker	948:953	arg1	presence					917:924	presence	917:924	presence	917:924	The data suggested that the secretion efficiency of these variants from the transfected cells was unaffected by the presence or absence of the CTP linker, N-glycans were attached to the analogs and the hCGβ-CTP domain in the FSHβCTPα variant was O-glycosylated.
25863346	1	47	theme	analogs	204:210	arg1	analogs					204:210	analogs	204:210	analogs	204:210	Single-chain (SC) gonadotropins have been genetically engineered to increase the repertoire of analogs for potential use in humans and domestic animals.
25863346	1	47	theme	analogs	204:210	arg1	repertoire					190:199	the repertoire	186:199	the repertoire of analogs	186:210	Single-chain (SC) gonadotropins have been genetically engineered to increase the repertoire of analogs for potential use in humans and domestic animals.
25863346	0	48	theme	related	32:38	arg1	activity					40:47	the steroidogenic related activity	14:47	the steroidogenic related activity	14:47	Modulation of the steroidogenic related activity according to the design of single-chain bovine FSH analogs.
25863346	9	49	theme	activation	1608:1617	arg1	analysis					1587:1594	A systematic analysis	1574:1594	A systematic analysis of receptor activation with ligands carrying structural differences	1574:1662	A systematic analysis of receptor activation with ligands carrying structural differences may identify new regulatory factor/s involved in the pleiotropic FSH activity.
25863346	2	50	contain	carrying	365:372	arg1	analogs					357:363	SC FSH analogs	350:363	SC FSH analogs carrying structural differences	350:395	The major aim of the current study was to examine the steroidogenic related activity of SC FSH analogs carrying structural differences.
25863346	2	50	contain	carrying	365:372	arg2	differences					385:395	structural differences	374:395	structural differences	374:395	The major aim of the current study was to examine the steroidogenic related activity of SC FSH analogs carrying structural differences.
25863346	6	51	theme	Cyp11a	1257:1262	arg1	transcripts					1317:1327	StAR, Cyp11a (P450scc), Cyp17 (P450c17) and Cyp19 (P450aromatase) transcripts	1251:1327	StAR, Cyp11a (P450scc), Cyp17 (P450c17) and Cyp19 (P450aromatase) transcripts	1251:1327	In immature mice, the analogs increased the ovary weight and induced StAR, Cyp11a (P450scc), Cyp17 (P450c17) and Cyp19 (P450aromatase) transcripts.
25863346	9	52	theme	pleiotropic	1717:1727	arg1	activity					1733:1740	the pleiotropic FSH activity	1713:1740	the pleiotropic FSH activity	1713:1740	A systematic analysis of receptor activation with ligands carrying structural differences may identify new regulatory factor/s involved in the pleiotropic FSH activity.
25863346	6	53	theme	immature	1185:1192	arg1	mice					1194:1197	immature mice	1185:1197	immature mice	1185:1197	In immature mice, the analogs increased the ovary weight and induced StAR, Cyp11a (P450scc), Cyp17 (P450c17) and Cyp19 (P450aromatase) transcripts.
25863346	4	54	theme	variants	859:866	arg1	efficiency					839:848	the secretion efficiency	825:848	the secretion efficiency of these variants from the transfected cells	825:893	The data suggested that the secretion efficiency of these variants from the transfected cells was unaffected by the presence or absence of the CTP linker, N-glycans were attached to the analogs and the hCGβ-CTP domain in the FSHβCTPα variant was O-glycosylated.
25863346	9	55	contain	carrying	1632:1639	arg2	differences					1652:1662	structural differences	1641:1662	structural differences	1641:1662	A systematic analysis of receptor activation with ligands carrying structural differences may identify new regulatory factor/s involved in the pleiotropic FSH activity.
25863346	9	55	contain	carrying	1632:1639	arg1	ligands					1624:1630	ligands	1624:1630	ligands carrying structural differences	1624:1662	A systematic analysis of receptor activation with ligands carrying structural differences may identify new regulatory factor/s involved in the pleiotropic FSH activity.
25863346	3	56	theme	subunit	521:527	arg1	domains					529:535	the tethered subunit domains	508:535	the tethered subunit domains	508:535	To address this issue, we designed and expressed three SC bovine FSH analogs in CHO cells: (i) FSHβα in which the tethered subunit domains are linked in tandem; (ii) FSHβCTPα that contains the carboxy terminal peptide (CTP) of the human choriogonadotropin (hCG) β subunit as a spacer, and (iii) FSHβboCTPα in which the linker is derived from a CTP-like sequence (boCTP) decoded from the bovine LHβ DNA.
25863346	4	57	theme	hCGβ-CTP	1003:1010	arg1	domain					1012:1017	the hCGβ-CTP domain	999:1017	the hCGβ-CTP domain in the FSHβCTPα variant	999:1041	The data suggested that the secretion efficiency of these variants from the transfected cells was unaffected by the presence or absence of the CTP linker, N-glycans were attached to the analogs and the hCGβ-CTP domain in the FSHβCTPα variant was O-glycosylated.
25863346	4	57	theme	hCGβ-CTP	1003:1010	arg1	O-glycosylated					1047:1060	O-glycosylated	1047:1060	O-glycosylated	1047:1060	The data suggested that the secretion efficiency of these variants from the transfected cells was unaffected by the presence or absence of the CTP linker, N-glycans were attached to the analogs and the hCGβ-CTP domain in the FSHβCTPα variant was O-glycosylated.
25863346	1	58	theme	Single-chain	109:120	arg1	SC					123:124	SC	123:124	SC	123:124	Single-chain (SC) gonadotropins have been genetically engineered to increase the repertoire of analogs for potential use in humans and domestic animals.
25863346	1	58	theme	Single-chain	109:120	arg1	gonadotropins					127:139	Single-chain (SC) gonadotropins	109:139	Single-chain (SC) gonadotropins	109:139	Single-chain (SC) gonadotropins have been genetically engineered to increase the repertoire of analogs for potential use in humans and domestic animals.
25863346	3	59	theme	subunit	662:668	arg1	CTP					617:619	CTP	617:619	CTP	617:619	To address this issue, we designed and expressed three SC bovine FSH analogs in CHO cells: (i) FSHβα in which the tethered subunit domains are linked in tandem; (ii) FSHβCTPα that contains the carboxy terminal peptide (CTP) of the human choriogonadotropin (hCG) β subunit as a spacer, and (iii) FSHβboCTPα in which the linker is derived from a CTP-like sequence (boCTP) decoded from the bovine LHβ DNA.
25863346	3	59	theme	subunit	662:668	arg1	peptide					608:614	the carboxy terminal peptide	587:614	the carboxy terminal peptide (CTP) of the human choriogonadotropin (hCG) β subunit	587:668	To address this issue, we designed and expressed three SC bovine FSH analogs in CHO cells: (i) FSHβα in which the tethered subunit domains are linked in tandem; (ii) FSHβCTPα that contains the carboxy terminal peptide (CTP) of the human choriogonadotropin (hCG) β subunit as a spacer, and (iii) FSHβboCTPα in which the linker is derived from a CTP-like sequence (boCTP) decoded from the bovine LHβ DNA.
25863346	3	59	theme	subunit	662:668	arg1	spacer					675:680	a spacer	673:680	a spacer	673:680	To address this issue, we designed and expressed three SC bovine FSH analogs in CHO cells: (i) FSHβα in which the tethered subunit domains are linked in tandem; (ii) FSHβCTPα that contains the carboxy terminal peptide (CTP) of the human choriogonadotropin (hCG) β subunit as a spacer, and (iii) FSHβboCTPα in which the linker is derived from a CTP-like sequence (boCTP) decoded from the bovine LHβ DNA.
25863346	3	60	theme	carboxy	591:597	arg1	CTP					617:619	CTP	617:619	CTP	617:619	To address this issue, we designed and expressed three SC bovine FSH analogs in CHO cells: (i) FSHβα in which the tethered subunit domains are linked in tandem; (ii) FSHβCTPα that contains the carboxy terminal peptide (CTP) of the human choriogonadotropin (hCG) β subunit as a spacer, and (iii) FSHβboCTPα in which the linker is derived from a CTP-like sequence (boCTP) decoded from the bovine LHβ DNA.
25863346	3	60	theme	carboxy	591:597	arg1	peptide					608:614	the carboxy terminal peptide	587:614	the carboxy terminal peptide (CTP) of the human choriogonadotropin (hCG) β subunit	587:668	To address this issue, we designed and expressed three SC bovine FSH analogs in CHO cells: (i) FSHβα in which the tethered subunit domains are linked in tandem; (ii) FSHβCTPα that contains the carboxy terminal peptide (CTP) of the human choriogonadotropin (hCG) β subunit as a spacer, and (iii) FSHβboCTPα in which the linker is derived from a CTP-like sequence (boCTP) decoded from the bovine LHβ DNA.
25863346	3	60	theme	carboxy	591:597	arg1	spacer					675:680	a spacer	673:680	a spacer	673:680	To address this issue, we designed and expressed three SC bovine FSH analogs in CHO cells: (i) FSHβα in which the tethered subunit domains are linked in tandem; (ii) FSHβCTPα that contains the carboxy terminal peptide (CTP) of the human choriogonadotropin (hCG) β subunit as a spacer, and (iii) FSHβboCTPα in which the linker is derived from a CTP-like sequence (boCTP) decoded from the bovine LHβ DNA.
25863346	3	61	dep	FSHβboCTPα	693:702	arg1	iii					688:690	iii	688:690	iii	688:690	To address this issue, we designed and expressed three SC bovine FSH analogs in CHO cells: (i) FSHβα in which the tethered subunit domains are linked in tandem; (ii) FSHβCTPα that contains the carboxy terminal peptide (CTP) of the human choriogonadotropin (hCG) β subunit as a spacer, and (iii) FSHβboCTPα in which the linker is derived from a CTP-like sequence (boCTP) decoded from the bovine LHβ DNA.
25863346	5	62	theme	progesterone	1151:1162	arg1	secretion					1164:1172	progesterone secretion	1151:1172	progesterone secretion	1151:1172	In a rat immortalized granulosa cell bioassay the potency of the three variants towards progesterone secretion varied.
25863346	6	63	theme	Cyp19	1295:1299	arg1	transcripts					1317:1327	StAR, Cyp11a (P450scc), Cyp17 (P450c17) and Cyp19 (P450aromatase) transcripts	1251:1327	StAR, Cyp11a (P450scc), Cyp17 (P450c17) and Cyp19 (P450aromatase) transcripts	1251:1327	In immature mice, the analogs increased the ovary weight and induced StAR, Cyp11a (P450scc), Cyp17 (P450c17) and Cyp19 (P450aromatase) transcripts.
25863346	4	64	dep	presence	917:924	arg1	the					913:915	the	913:915	the	913:915	The data suggested that the secretion efficiency of these variants from the transfected cells was unaffected by the presence or absence of the CTP linker, N-glycans were attached to the analogs and the hCGβ-CTP domain in the FSHβCTPα variant was O-glycosylated.
25863346	3	65	theme	terminal	599:606	arg1	CTP					617:619	CTP	617:619	CTP	617:619	To address this issue, we designed and expressed three SC bovine FSH analogs in CHO cells: (i) FSHβα in which the tethered subunit domains are linked in tandem; (ii) FSHβCTPα that contains the carboxy terminal peptide (CTP) of the human choriogonadotropin (hCG) β subunit as a spacer, and (iii) FSHβboCTPα in which the linker is derived from a CTP-like sequence (boCTP) decoded from the bovine LHβ DNA.
25863346	3	65	theme	terminal	599:606	arg1	peptide					608:614	the carboxy terminal peptide	587:614	the carboxy terminal peptide (CTP) of the human choriogonadotropin (hCG) β subunit	587:668	To address this issue, we designed and expressed three SC bovine FSH analogs in CHO cells: (i) FSHβα in which the tethered subunit domains are linked in tandem; (ii) FSHβCTPα that contains the carboxy terminal peptide (CTP) of the human choriogonadotropin (hCG) β subunit as a spacer, and (iii) FSHβboCTPα in which the linker is derived from a CTP-like sequence (boCTP) decoded from the bovine LHβ DNA.
25863346	3	65	theme	terminal	599:606	arg1	spacer					675:680	a spacer	673:680	a spacer	673:680	To address this issue, we designed and expressed three SC bovine FSH analogs in CHO cells: (i) FSHβα in which the tethered subunit domains are linked in tandem; (ii) FSHβCTPα that contains the carboxy terminal peptide (CTP) of the human choriogonadotropin (hCG) β subunit as a spacer, and (iii) FSHβboCTPα in which the linker is derived from a CTP-like sequence (boCTP) decoded from the bovine LHβ DNA.
25863346	3	66	attach	derived	727:733	arg1	boCTP					761:765	boCTP	761:765	boCTP	761:765	To address this issue, we designed and expressed three SC bovine FSH analogs in CHO cells: (i) FSHβα in which the tethered subunit domains are linked in tandem; (ii) FSHβCTPα that contains the carboxy terminal peptide (CTP) of the human choriogonadotropin (hCG) β subunit as a spacer, and (iii) FSHβboCTPα in which the linker is derived from a CTP-like sequence (boCTP) decoded from the bovine LHβ DNA.
25863346	3	66	attach	derived	727:733	arg2	linker					717:722	the linker	713:722	the linker	713:722	To address this issue, we designed and expressed three SC bovine FSH analogs in CHO cells: (i) FSHβα in which the tethered subunit domains are linked in tandem; (ii) FSHβCTPα that contains the carboxy terminal peptide (CTP) of the human choriogonadotropin (hCG) β subunit as a spacer, and (iii) FSHβboCTPα in which the linker is derived from a CTP-like sequence (boCTP) decoded from the bovine LHβ DNA.
25863346	3	66	attach	derived	727:733	arg1	sequence					751:758	a CTP-like sequence	740:758	a CTP-like sequence (boCTP) decoded from the bovine LHβ DNA	740:798	To address this issue, we designed and expressed three SC bovine FSH analogs in CHO cells: (i) FSHβα in which the tethered subunit domains are linked in tandem; (ii) FSHβCTPα that contains the carboxy terminal peptide (CTP) of the human choriogonadotropin (hCG) β subunit as a spacer, and (iii) FSHβboCTPα in which the linker is derived from a CTP-like sequence (boCTP) decoded from the bovine LHβ DNA.
25863346	3	67	theme	SC	453:454	arg1	analogs					467:473	three SC bovine FSH analogs	447:473	three SC bovine FSH analogs in CHO cells: (i) FSHβα in which the tethered subunit domains are linked in tandem; (ii) FSHβCTPα that contains the carboxy terminal peptide (CTP) of the human choriogonadotropin (hCG) β subunit as a spacer, and (iii) FSHβboCTPα in which the linker is derived from a CTP-like sequence (boCTP) decoded from the bovine LHβ DNA	447:798	To address this issue, we designed and expressed three SC bovine FSH analogs in CHO cells: (i) FSHβα in which the tethered subunit domains are linked in tandem; (ii) FSHβCTPα that contains the carboxy terminal peptide (CTP) of the human choriogonadotropin (hCG) β subunit as a spacer, and (iii) FSHβboCTPα in which the linker is derived from a CTP-like sequence (boCTP) decoded from the bovine LHβ DNA.
25863346	9	68	theme	structural	1641:1650	arg1	differences					1652:1662	structural differences	1641:1662	structural differences	1641:1662	A systematic analysis of receptor activation with ligands carrying structural differences may identify new regulatory factor/s involved in the pleiotropic FSH activity.
25863346	1	69	theme	potential	216:224	arg1	use					226:228	potential use	216:228	potential use in humans and domestic animals	216:259	Single-chain (SC) gonadotropins have been genetically engineered to increase the repertoire of analogs for potential use in humans and domestic animals.
25863346	1	70	from	use	226:228	arg1	humans					233:238	humans	233:238	humans	233:238	Single-chain (SC) gonadotropins have been genetically engineered to increase the repertoire of analogs for potential use in humans and domestic animals.
25863346	1	70	from	use	226:228	arg1	animals					253:259	domestic animals	244:259	domestic animals	244:259	Single-chain (SC) gonadotropins have been genetically engineered to increase the repertoire of analogs for potential use in humans and domestic animals.
25863346	3	71	theme	bovine	456:461	arg1	analogs					467:473	three SC bovine FSH analogs	447:473	three SC bovine FSH analogs in CHO cells: (i) FSHβα in which the tethered subunit domains are linked in tandem; (ii) FSHβCTPα that contains the carboxy terminal peptide (CTP) of the human choriogonadotropin (hCG) β subunit as a spacer, and (iii) FSHβboCTPα in which the linker is derived from a CTP-like sequence (boCTP) decoded from the bovine LHβ DNA	447:798	To address this issue, we designed and expressed three SC bovine FSH analogs in CHO cells: (i) FSHβα in which the tethered subunit domains are linked in tandem; (ii) FSHβCTPα that contains the carboxy terminal peptide (CTP) of the human choriogonadotropin (hCG) β subunit as a spacer, and (iii) FSHβboCTPα in which the linker is derived from a CTP-like sequence (boCTP) decoded from the bovine LHβ DNA.
25863346	4	72	gly	O-glycosylated	1047:1060	arg1	domain					1012:1017	the hCGβ-CTP domain	999:1017	the hCGβ-CTP domain in the FSHβCTPα variant	999:1041	The data suggested that the secretion efficiency of these variants from the transfected cells was unaffected by the presence or absence of the CTP linker, N-glycans were attached to the analogs and the hCGβ-CTP domain in the FSHβCTPα variant was O-glycosylated.
25863346	4	72	gly	O-glycosylated	1047:1060	arg1	O-glycosylated					1047:1060	O-glycosylated	1047:1060	O-glycosylated	1047:1060	The data suggested that the secretion efficiency of these variants from the transfected cells was unaffected by the presence or absence of the CTP linker, N-glycans were attached to the analogs and the hCGβ-CTP domain in the FSHβCTPα variant was O-glycosylated.
25863346	7	73	theme	levels	1393:1398	arg1	amplitude					1363:1371	amplitude	1363:1371	amplitude	1363:1371	However, the dose dependence and amplitude of these transcript levels differed in response to FSHβα, FSHβboCTPα and FSHβCTPα.
25863346	7	73	theme	levels	1393:1398	arg1	dependence					1348:1357	the dose dependence	1339:1357	the dose dependence	1339:1357	However, the dose dependence and amplitude of these transcript levels differed in response to FSHβα, FSHβboCTPα and FSHβCTPα.
25863346	6	74	theme	ovary	1226:1230	arg1	weight					1232:1237	the ovary weight	1222:1237	the ovary weight	1222:1237	In immature mice, the analogs increased the ovary weight and induced StAR, Cyp11a (P450scc), Cyp17 (P450c17) and Cyp19 (P450aromatase) transcripts.
25863346	3	75	theme	CTP-like	742:749	arg1	boCTP					761:765	boCTP	761:765	boCTP	761:765	To address this issue, we designed and expressed three SC bovine FSH analogs in CHO cells: (i) FSHβα in which the tethered subunit domains are linked in tandem; (ii) FSHβCTPα that contains the carboxy terminal peptide (CTP) of the human choriogonadotropin (hCG) β subunit as a spacer, and (iii) FSHβboCTPα in which the linker is derived from a CTP-like sequence (boCTP) decoded from the bovine LHβ DNA.
25863346	3	75	theme	CTP-like	742:749	arg1	sequence					751:758	a CTP-like sequence	740:758	a CTP-like sequence (boCTP) decoded from the bovine LHβ DNA	740:798	To address this issue, we designed and expressed three SC bovine FSH analogs in CHO cells: (i) FSHβα in which the tethered subunit domains are linked in tandem; (ii) FSHβCTPα that contains the carboxy terminal peptide (CTP) of the human choriogonadotropin (hCG) β subunit as a spacer, and (iii) FSHβboCTPα in which the linker is derived from a CTP-like sequence (boCTP) decoded from the bovine LHβ DNA.
25863346	2	76	theme	SC	350:351	arg1	analogs					357:363	SC FSH analogs	350:363	SC FSH analogs carrying structural differences	350:395	The major aim of the current study was to examine the steroidogenic related activity of SC FSH analogs carrying structural differences.
25863346	9	77	theme	receptor	1599:1606	arg1	activation					1608:1617	receptor activation	1599:1617	receptor activation with ligands carrying structural differences	1599:1662	A systematic analysis of receptor activation with ligands carrying structural differences may identify new regulatory factor/s involved in the pleiotropic FSH activity.
25863346	3	78	dep	FSHβCTPα	564:571	arg1	ii					560:561	ii	560:561	ii	560:561	To address this issue, we designed and expressed three SC bovine FSH analogs in CHO cells: (i) FSHβα in which the tethered subunit domains are linked in tandem; (ii) FSHβCTPα that contains the carboxy terminal peptide (CTP) of the human choriogonadotropin (hCG) β subunit as a spacer, and (iii) FSHβboCTPα in which the linker is derived from a CTP-like sequence (boCTP) decoded from the bovine LHβ DNA.
25863346	7	79	theme	dose	1343:1346	arg1	dependence					1348:1357	the dose dependence	1339:1357	the dose dependence	1339:1357	However, the dose dependence and amplitude of these transcript levels differed in response to FSHβα, FSHβboCTPα and FSHβCTPα.
25863346	7	80	dep	FSHβα	1424:1428	arg1	response					1412:1419	response	1412:1419	response	1412:1419	However, the dose dependence and amplitude of these transcript levels differed in response to FSHβα, FSHβboCTPα and FSHβCTPα.
25863346	4	81	theme	transfected	877:887	arg1	cells					889:893	the transfected cells	873:893	the transfected cells	873:893	The data suggested that the secretion efficiency of these variants from the transfected cells was unaffected by the presence or absence of the CTP linker, N-glycans were attached to the analogs and the hCGβ-CTP domain in the FSHβCTPα variant was O-glycosylated.
25863346	7	82	theme	transcript	1382:1391	arg1	levels					1393:1398	these transcript levels	1376:1398	these transcript levels	1376:1398	However, the dose dependence and amplitude of these transcript levels differed in response to FSHβα, FSHβboCTPα and FSHβCTPα.
25863346	3	83	attach	linked	541:546	arg3	FSHβα					493:497	(i) FSHβα	489:497	three SC bovine FSH analogs in CHO cells: (i) FSHβα in which the tethered subunit domains are linked in tandem; (ii) FSHβCTPα that contains the carboxy terminal peptide (CTP) of the human choriogonadotropin (hCG) β subunit as a spacer, and (iii) FSHβboCTPα in which the linker is derived from a CTP-like sequence (boCTP) decoded from the bovine LHβ DNA	447:798	To address this issue, we designed and expressed three SC bovine FSH analogs in CHO cells: (i) FSHβα in which the tethered subunit domains are linked in tandem; (ii) FSHβCTPα that contains the carboxy terminal peptide (CTP) of the human choriogonadotropin (hCG) β subunit as a spacer, and (iii) FSHβboCTPα in which the linker is derived from a CTP-like sequence (boCTP) decoded from the bovine LHβ DNA.
25863346	3	83	attach	linked	541:546	arg2	domains					529:535	the tethered subunit domains	508:535	the tethered subunit domains	508:535	To address this issue, we designed and expressed three SC bovine FSH analogs in CHO cells: (i) FSHβα in which the tethered subunit domains are linked in tandem; (ii) FSHβCTPα that contains the carboxy terminal peptide (CTP) of the human choriogonadotropin (hCG) β subunit as a spacer, and (iii) FSHβboCTPα in which the linker is derived from a CTP-like sequence (boCTP) decoded from the bovine LHβ DNA.
25863346	3	83	attach	linked	541:546	arg3	tandem					551:556	tandem	551:556	tandem	551:556	To address this issue, we designed and expressed three SC bovine FSH analogs in CHO cells: (i) FSHβα in which the tethered subunit domains are linked in tandem; (ii) FSHβCTPα that contains the carboxy terminal peptide (CTP) of the human choriogonadotropin (hCG) β subunit as a spacer, and (iii) FSHβboCTPα in which the linker is derived from a CTP-like sequence (boCTP) decoded from the bovine LHβ DNA.
25863346	5	84	theme	rat	1068:1070	arg1	bioassay					1100:1107	a rat immortalized granulosa cell bioassay	1066:1107	a rat immortalized granulosa cell bioassay	1066:1107	In a rat immortalized granulosa cell bioassay the potency of the three variants towards progesterone secretion varied.
26917395	2	0	from	selenium	425:432	arg1	medium					449:454	the culture medium	437:454	the culture medium	437:454	The end product of metabolic process is selenium-bearing exopolysaccharide (Se-EPS), which contains a higher content of uronic acid than the exopolysaccharide (EPS) by the strain without selenium in the culture medium.
26917395	6	1	with	mannose	906:912	arg1	ratio					950:954	a molar ratio	942:954	a molar ratio of 19.58:19.28:5.97:18.99:23.70:12.48, respectively	942:1006	Results showed that Se-EPS-2 was a homogenous polysaccharide with molecular weight of 7.3 kDa, consisting of monosaccharides, rhamnose, arabinose, xylose, mannose, glucose and galactose with a molar ratio of 19.58:19.28:5.97:18.99:23.70:12.48, respectively.
26917395	3	2	theme	mg/kg	517:521	arg1	yield					502:506	a maximum yield	492:506	a maximum yield of 256.7 mg/kg	492:521	Selenium content in Se-EPS reached a maximum yield of 256.7 mg/kg when using an optimized culture condition.
26917395	3	3	theme	optimized	537:545	arg1	condition					555:563	an optimized culture condition	534:563	an optimized culture condition	534:563	Selenium content in Se-EPS reached a maximum yield of 256.7 mg/kg when using an optimized culture condition.
26917395	5	4	theme	chemical	671:678	arg1	composition					680:690	chemical composition	671:690	chemical composition	671:690	Structure and chemical composition of Se-EPS-2 were investigated by chromatographic analyses.
26917395	3	5	theme	culture	547:553	arg1	condition					555:563	an optimized culture condition	534:563	an optimized culture condition	534:563	Selenium content in Se-EPS reached a maximum yield of 256.7 mg/kg when using an optimized culture condition.
26917395	0	6	theme	antioxidant	105:115	arg1	activities					117:126	its antioxidant activities	101:126	its antioxidant activities	101:126	Biosynthesis of selenium rich exopolysaccharide (Se-EPS) by Pseudomonas PT-8 and characterization of its antioxidant activities.
26917395	3	7	theme	maximum	494:500	arg1	yield					502:506	a maximum yield	492:506	a maximum yield of 256.7 mg/kg	492:521	Selenium content in Se-EPS reached a maximum yield of 256.7 mg/kg when using an optimized culture condition.
26917395	1	8	theme	submerged	176:184	arg1	fermentation					186:197	submerged fermentation	176:197	submerged fermentation of selenium-tolerant Pseudomonas PT-8	176:235	Biosynthesis of organo-selenium is achieved by submerged fermentation of selenium-tolerant Pseudomonas PT-8.
26917395	7	9	theme	rhamnose	1045:1052	arg1	content					1034:1040	the content	1030:1040	the content of rhamnose in Se-EPS	1030:1062	Compared to the EPS, the content of rhamnose in Se-EPS increased and molecular weight decreased.
26917395	6	10	theme	19.58:19.28:5.97:18.99:23.70:12.48	959:992	arg1	ratio					950:954	a molar ratio	942:954	a molar ratio of 19.58:19.28:5.97:18.99:23.70:12.48, respectively	942:1006	Results showed that Se-EPS-2 was a homogenous polysaccharide with molecular weight of 7.3 kDa, consisting of monosaccharides, rhamnose, arabinose, xylose, mannose, glucose and galactose with a molar ratio of 19.58:19.28:5.97:18.99:23.70:12.48, respectively.
26917395	6	11	theme	molecular	817:825	arg1	weight					827:832	molecular weight	817:832	molecular weight of 7.3 kDa	817:843	Results showed that Se-EPS-2 was a homogenous polysaccharide with molecular weight of 7.3 kDa, consisting of monosaccharides, rhamnose, arabinose, xylose, mannose, glucose and galactose with a molar ratio of 19.58:19.28:5.97:18.99:23.70:12.48, respectively.
26917395	2	12	theme	higher	340:345	arg1	content					347:353	a higher content	338:353	a higher content of uronic acid than the exopolysaccharide (EPS) by the strain without selenium in the culture medium	338:454	The end product of metabolic process is selenium-bearing exopolysaccharide (Se-EPS), which contains a higher content of uronic acid than the exopolysaccharide (EPS) by the strain without selenium in the culture medium.
26917395	7	13	theme	molecular	1078:1086	arg1	weight					1088:1093	molecular weight	1078:1093	molecular weight	1078:1093	Compared to the EPS, the content of rhamnose in Se-EPS increased and molecular weight decreased.
26917395	0	14	dep	PT-8	72:75	arg1	Pseudomonas					60:70	Pseudomonas PT-8 and characterization of its antioxidant activities	60:126	Pseudomonas PT-8 and characterization of its antioxidant activities	60:126	Biosynthesis of selenium rich exopolysaccharide (Se-EPS) by Pseudomonas PT-8 and characterization of its antioxidant activities.
26917395	1	15	theme	selenium-tolerant	202:218	arg1	PT-8					232:235	selenium-tolerant Pseudomonas PT-8	202:235	selenium-tolerant Pseudomonas PT-8	202:235	Biosynthesis of organo-selenium is achieved by submerged fermentation of selenium-tolerant Pseudomonas PT-8.
26917395	2	16	theme	culture	441:447	arg1	medium					449:454	the culture medium	437:454	the culture medium	437:454	The end product of metabolic process is selenium-bearing exopolysaccharide (Se-EPS), which contains a higher content of uronic acid than the exopolysaccharide (EPS) by the strain without selenium in the culture medium.
26917395	1	17	theme	Pseudomonas	220:230	arg1	PT-8					232:235	selenium-tolerant Pseudomonas PT-8	202:235	selenium-tolerant Pseudomonas PT-8	202:235	Biosynthesis of organo-selenium is achieved by submerged fermentation of selenium-tolerant Pseudomonas PT-8.
26917395	0	18	theme	selenium	16:23	arg1	Se-EPS					49:54	selenium rich exopolysaccharide (Se-EPS)	16:55	selenium rich exopolysaccharide (Se-EPS)	16:55	Biosynthesis of selenium rich exopolysaccharide (Se-EPS) by Pseudomonas PT-8 and characterization of its antioxidant activities.
26917395	0	19	theme	activities	117:126	arg1	PT-8					72:75	PT-8	72:75	PT-8	72:75	Biosynthesis of selenium rich exopolysaccharide (Se-EPS) by Pseudomonas PT-8 and characterization of its antioxidant activities.
26917395	0	19	theme	activities	117:126	arg1	characterization					81:96	characterization	81:96	characterization	81:96	Biosynthesis of selenium rich exopolysaccharide (Se-EPS) by Pseudomonas PT-8 and characterization of its antioxidant activities.
26917395	1	20	theme	PT-8	232:235	arg1	fermentation					186:197	submerged fermentation	176:197	submerged fermentation of selenium-tolerant Pseudomonas PT-8	176:235	Biosynthesis of organo-selenium is achieved by submerged fermentation of selenium-tolerant Pseudomonas PT-8.
26917395	4	21	theme	Crude	566:570	arg1	Se-EPS					572:577	Crude Se-EPS	566:577	Crude Se-EPS	566:577	Crude Se-EPS was purified into two fractions-a pH neutral Se-EPS-1 and an acidic Se-EPS-2.
26917395	5	22	theme	chromatographic	725:739	arg1	analyses					741:748	chromatographic analyses	725:748	chromatographic analyses	725:748	Structure and chemical composition of Se-EPS-2 were investigated by chromatographic analyses.
26917395	7	23	from	content	1034:1040	arg1	Se-EPS					1057:1062	Se-EPS	1057:1062	Se-EPS	1057:1062	Compared to the EPS, the content of rhamnose in Se-EPS increased and molecular weight decreased.
26917395	2	24	theme	metabolic	257:265	arg1	process					267:273	metabolic process	257:273	metabolic process	257:273	The end product of metabolic process is selenium-bearing exopolysaccharide (Se-EPS), which contains a higher content of uronic acid than the exopolysaccharide (EPS) by the strain without selenium in the culture medium.
26917395	3	25	theme	Selenium	457:464	arg1	content					466:472	Selenium content	457:472	Selenium content in Se-EPS	457:482	Selenium content in Se-EPS reached a maximum yield of 256.7 mg/kg when using an optimized culture condition.
26917395	0	26	theme	exopolysaccharide	30:46	arg1	Se-EPS					49:54	selenium rich exopolysaccharide (Se-EPS)	16:55	selenium rich exopolysaccharide (Se-EPS)	16:55	Biosynthesis of selenium rich exopolysaccharide (Se-EPS) by Pseudomonas PT-8 and characterization of its antioxidant activities.
26917395	6	27	with	polysaccharide	797:810	arg1	weight					827:832	molecular weight	817:832	molecular weight of 7.3 kDa	817:843	Results showed that Se-EPS-2 was a homogenous polysaccharide with molecular weight of 7.3 kDa, consisting of monosaccharides, rhamnose, arabinose, xylose, mannose, glucose and galactose with a molar ratio of 19.58:19.28:5.97:18.99:23.70:12.48, respectively.
26917395	0	28	theme	rich	25:28	arg1	Se-EPS					49:54	selenium rich exopolysaccharide (Se-EPS)	16:55	selenium rich exopolysaccharide (Se-EPS)	16:55	Biosynthesis of selenium rich exopolysaccharide (Se-EPS) by Pseudomonas PT-8 and characterization of its antioxidant activities.
26917395	6	29	theme	homogenous	786:795	arg1	polysaccharide					797:810	a homogenous polysaccharide	784:810	a homogenous polysaccharide	784:810	Results showed that Se-EPS-2 was a homogenous polysaccharide with molecular weight of 7.3 kDa, consisting of monosaccharides, rhamnose, arabinose, xylose, mannose, glucose and galactose with a molar ratio of 19.58:19.28:5.97:18.99:23.70:12.48, respectively.
26917395	6	29	theme	homogenous	786:795	arg1	Se-EPS-2					771:778	Se-EPS-2	771:778	Se-EPS-2	771:778	Results showed that Se-EPS-2 was a homogenous polysaccharide with molecular weight of 7.3 kDa, consisting of monosaccharides, rhamnose, arabinose, xylose, mannose, glucose and galactose with a molar ratio of 19.58:19.28:5.97:18.99:23.70:12.48, respectively.
26917395	4	30	theme	acidic	640:645	arg1	Se-EPS-2					647:654	an acidic Se-EPS-2	637:654	an acidic Se-EPS-2	637:654	Crude Se-EPS was purified into two fractions-a pH neutral Se-EPS-1 and an acidic Se-EPS-2.
26917395	2	31	contain	contains	329:336	arg1	Se-EPS					314:319	Se-EPS	314:319	Se-EPS	314:319	The end product of metabolic process is selenium-bearing exopolysaccharide (Se-EPS), which contains a higher content of uronic acid than the exopolysaccharide (EPS) by the strain without selenium in the culture medium.
26917395	2	31	contain	contains	329:336	arg2	content					347:353	a higher content	338:353	a higher content of uronic acid than the exopolysaccharide (EPS) by the strain without selenium in the culture medium	338:454	The end product of metabolic process is selenium-bearing exopolysaccharide (Se-EPS), which contains a higher content of uronic acid than the exopolysaccharide (EPS) by the strain without selenium in the culture medium.
26917395	2	31	contain	contains	329:336	arg1	product					246:252	The end product	238:252	The end product of metabolic process	238:273	The end product of metabolic process is selenium-bearing exopolysaccharide (Se-EPS), which contains a higher content of uronic acid than the exopolysaccharide (EPS) by the strain without selenium in the culture medium.
26917395	2	31	contain	contains	329:336	arg1	exopolysaccharide					295:311	selenium-bearing exopolysaccharide	278:311	selenium-bearing exopolysaccharide (Se-EPS)	278:320	The end product of metabolic process is selenium-bearing exopolysaccharide (Se-EPS), which contains a higher content of uronic acid than the exopolysaccharide (EPS) by the strain without selenium in the culture medium.
26917395	8	32	theme	scavenging	1128:1137	arg1	actions					1139:1145	strong scavenging actions	1121:1145	strong scavenging actions	1121:1145	The Se-EPS had strong scavenging actions on DPPH•, •OH and •O2(-), which is much higher than the EPS.
26917395	3	33	from	content	466:472	arg1	Se-EPS					477:482	Se-EPS	477:482	Se-EPS	477:482	Selenium content in Se-EPS reached a maximum yield of 256.7 mg/kg when using an optimized culture condition.
26917395	2	34	theme	acid	365:368	arg1	content					347:353	a higher content	338:353	a higher content of uronic acid than the exopolysaccharide (EPS) by the strain without selenium in the culture medium	338:454	The end product of metabolic process is selenium-bearing exopolysaccharide (Se-EPS), which contains a higher content of uronic acid than the exopolysaccharide (EPS) by the strain without selenium in the culture medium.
26917395	0	35	theme	Se-EPS	49:54	arg1	Biosynthesis					0:11	Biosynthesis	0:11	Biosynthesis of selenium rich exopolysaccharide (Se-EPS) by Pseudomonas PT-8 and characterization of its antioxidant activities.	0:127	Biosynthesis of selenium rich exopolysaccharide (Se-EPS) by Pseudomonas PT-8 and characterization of its antioxidant activities.
26917395	6	36	theme	molar	944:948	arg1	ratio					950:954	a molar ratio	942:954	a molar ratio of 19.58:19.28:5.97:18.99:23.70:12.48, respectively	942:1006	Results showed that Se-EPS-2 was a homogenous polysaccharide with molecular weight of 7.3 kDa, consisting of monosaccharides, rhamnose, arabinose, xylose, mannose, glucose and galactose with a molar ratio of 19.58:19.28:5.97:18.99:23.70:12.48, respectively.
26917395	8	37	theme	strong	1121:1126	arg1	actions					1139:1145	strong scavenging actions	1121:1145	strong scavenging actions	1121:1145	The Se-EPS had strong scavenging actions on DPPH•, •OH and •O2(-), which is much higher than the EPS.
26917395	2	38	theme	end	242:244	arg1	product					246:252	The end product	238:252	The end product of metabolic process	238:273	The end product of metabolic process is selenium-bearing exopolysaccharide (Se-EPS), which contains a higher content of uronic acid than the exopolysaccharide (EPS) by the strain without selenium in the culture medium.
26917395	2	38	theme	end	242:244	arg1	exopolysaccharide					295:311	selenium-bearing exopolysaccharide	278:311	selenium-bearing exopolysaccharide (Se-EPS)	278:320	The end product of metabolic process is selenium-bearing exopolysaccharide (Se-EPS), which contains a higher content of uronic acid than the exopolysaccharide (EPS) by the strain without selenium in the culture medium.
26917395	2	39	theme	uronic	358:363	arg1	acid					365:368	uronic acid	358:368	uronic acid	358:368	The end product of metabolic process is selenium-bearing exopolysaccharide (Se-EPS), which contains a higher content of uronic acid than the exopolysaccharide (EPS) by the strain without selenium in the culture medium.
26917395	5	40	theme	Se-EPS-2	695:702	arg1	Structure					657:665	Structure	657:665	Structure	657:665	Structure and chemical composition of Se-EPS-2 were investigated by chromatographic analyses.
26917395	5	40	theme	Se-EPS-2	695:702	arg1	composition					680:690	chemical composition	671:690	chemical composition	671:690	Structure and chemical composition of Se-EPS-2 were investigated by chromatographic analyses.
26917395	6	41	theme	kDa	841:843	arg1	weight					827:832	molecular weight	817:832	molecular weight of 7.3 kDa	817:843	Results showed that Se-EPS-2 was a homogenous polysaccharide with molecular weight of 7.3 kDa, consisting of monosaccharides, rhamnose, arabinose, xylose, mannose, glucose and galactose with a molar ratio of 19.58:19.28:5.97:18.99:23.70:12.48, respectively.
26917395	4	42	theme	pH	613:614	arg1	Se-EPS-1					624:631	two fractions-a pH neutral Se-EPS-1	597:631	two fractions-a pH neutral Se-EPS-1	597:631	Crude Se-EPS was purified into two fractions-a pH neutral Se-EPS-1 and an acidic Se-EPS-2.
26917395	6	43	with	glucose	915:921	arg1	ratio					950:954	a molar ratio	942:954	a molar ratio of 19.58:19.28:5.97:18.99:23.70:12.48, respectively	942:1006	Results showed that Se-EPS-2 was a homogenous polysaccharide with molecular weight of 7.3 kDa, consisting of monosaccharides, rhamnose, arabinose, xylose, mannose, glucose and galactose with a molar ratio of 19.58:19.28:5.97:18.99:23.70:12.48, respectively.
26917395	2	44	theme	selenium-bearing	278:293	arg1	Se-EPS					314:319	Se-EPS	314:319	Se-EPS	314:319	The end product of metabolic process is selenium-bearing exopolysaccharide (Se-EPS), which contains a higher content of uronic acid than the exopolysaccharide (EPS) by the strain without selenium in the culture medium.
26917395	2	44	theme	selenium-bearing	278:293	arg1	product					246:252	The end product	238:252	The end product of metabolic process	238:273	The end product of metabolic process is selenium-bearing exopolysaccharide (Se-EPS), which contains a higher content of uronic acid than the exopolysaccharide (EPS) by the strain without selenium in the culture medium.
26917395	2	44	theme	selenium-bearing	278:293	arg1	exopolysaccharide					295:311	selenium-bearing exopolysaccharide	278:311	selenium-bearing exopolysaccharide (Se-EPS)	278:320	The end product of metabolic process is selenium-bearing exopolysaccharide (Se-EPS), which contains a higher content of uronic acid than the exopolysaccharide (EPS) by the strain without selenium in the culture medium.
26917395	6	45	with	galactose	927:935	arg1	ratio					950:954	a molar ratio	942:954	a molar ratio of 19.58:19.28:5.97:18.99:23.70:12.48, respectively	942:1006	Results showed that Se-EPS-2 was a homogenous polysaccharide with molecular weight of 7.3 kDa, consisting of monosaccharides, rhamnose, arabinose, xylose, mannose, glucose and galactose with a molar ratio of 19.58:19.28:5.97:18.99:23.70:12.48, respectively.
26917395	4	46	theme	fractions-a	601:611	arg1	Se-EPS-1					624:631	two fractions-a pH neutral Se-EPS-1	597:631	two fractions-a pH neutral Se-EPS-1	597:631	Crude Se-EPS was purified into two fractions-a pH neutral Se-EPS-1 and an acidic Se-EPS-2.
26917395	1	47	theme	organo-selenium	145:159	arg1	Biosynthesis					129:140	Biosynthesis	129:140	Biosynthesis of organo-selenium	129:159	Biosynthesis of organo-selenium is achieved by submerged fermentation of selenium-tolerant Pseudomonas PT-8.
26917395	2	48	theme	process	267:273	arg1	product					246:252	The end product	238:252	The end product of metabolic process	238:273	The end product of metabolic process is selenium-bearing exopolysaccharide (Se-EPS), which contains a higher content of uronic acid than the exopolysaccharide (EPS) by the strain without selenium in the culture medium.
26917395	2	48	theme	process	267:273	arg1	exopolysaccharide					295:311	selenium-bearing exopolysaccharide	278:311	selenium-bearing exopolysaccharide (Se-EPS)	278:320	The end product of metabolic process is selenium-bearing exopolysaccharide (Se-EPS), which contains a higher content of uronic acid than the exopolysaccharide (EPS) by the strain without selenium in the culture medium.
26917395	8	49	contain	had	1117:1119	arg1	Se-EPS					1110:1115	The Se-EPS	1106:1115	The Se-EPS	1106:1115	The Se-EPS had strong scavenging actions on DPPH•, •OH and •O2(-), which is much higher than the EPS.
26917395	8	49	contain	had	1117:1119	arg2	actions					1139:1145	strong scavenging actions	1121:1145	strong scavenging actions	1121:1145	The Se-EPS had strong scavenging actions on DPPH•, •OH and •O2(-), which is much higher than the EPS.
26917395	4	50	theme	neutral	616:622	arg1	Se-EPS-1					624:631	two fractions-a pH neutral Se-EPS-1	597:631	two fractions-a pH neutral Se-EPS-1	597:631	Crude Se-EPS was purified into two fractions-a pH neutral Se-EPS-1 and an acidic Se-EPS-2.
27295251	4	0	theme	Lewis	589:593	arg1	acid					595:598	the Lewis acid	585:598	the Lewis acid	585:598	Surface area and pore volume increased with the hardness of the Lewis acid, in correspondence with the decrease of the amount of lignin and hemicellulose in the pulp.
27295251	7	1	theme	/g	980:981	arg1	OM					983:984	295Ncm(3)/g OM	971:984	295Ncm(3)/g OM	971:984	A methane evolution of 295Ncm(3)/g OM was reached by a moderate improvement of the accessibility of the native straw.
27295251	0	2	theme	Lewis	71:75	arg1	acids					77:81	Lewis acids	71:81	Lewis acids	71:81	Composition, texture and methane potential of cellulosic residues from Lewis acids organosolv pulping of wheat straw.
27295251	4	3	theme	pore	542:545	arg1	volume					547:552	pore volume	542:552	pore volume	542:552	Surface area and pore volume increased with the hardness of the Lewis acid, in correspondence with the decrease of the amount of lignin and hemicellulose in the pulp.
27295251	5	4	theme	fibrils	806:812	arg1	agglomeration					779:791	an agglomeration	776:791	an agglomeration of cellulose fibrils	776:812	The non linearity of the correlation between porosity and composition suggests that an agglomeration of cellulose fibrils occurs in the early stages of pulping.
27295251	4	5	theme	acid	595:598	arg1	hardness					573:580	the hardness	569:580	the hardness of the Lewis acid	569:598	Surface area and pore volume increased with the hardness of the Lewis acid, in correspondence with the decrease of the amount of lignin and hemicellulose in the pulp.
27295251	4	6	from	pulp	686:689	arg1	decrease					628:635	the decrease	624:635	the decrease of the amount of lignin and hemicellulose in the pulp	624:689	Surface area and pore volume increased with the hardness of the Lewis acid, in correspondence with the decrease of the amount of lignin and hemicellulose in the pulp.
27295251	3	7	theme	chemical	374:381	arg1	composition					383:393	chemical composition	374:393	chemical composition	374:393	The cellulosic residue was characterised by chemical composition, X-ray diffraction, FT-IR spectroscopy, N2 physisorption, scanning electron microscopy and potential for anaerobic digestibility.
27295251	7	8	theme	moderate	1003:1010	arg1	improvement					1012:1022	a moderate improvement	1001:1022	a moderate improvement of the accessibility of the native straw	1001:1063	A methane evolution of 295Ncm(3)/g OM was reached by a moderate improvement of the accessibility of the native straw.
27295251	7	9	theme	straw	1059:1063	arg1	accessibility					1031:1043	the accessibility	1027:1043	the accessibility of the native straw	1027:1063	A methane evolution of 295Ncm(3)/g OM was reached by a moderate improvement of the accessibility of the native straw.
27295251	4	10	theme	lignin	654:659	arg1	hemicellulose					665:677	hemicellulose	665:677	hemicellulose	665:677	Surface area and pore volume increased with the hardness of the Lewis acid, in correspondence with the decrease of the amount of lignin and hemicellulose in the pulp.
27295251	4	10	theme	lignin	654:659	arg1	lignin					654:659	lignin	654:659	lignin	654:659	Surface area and pore volume increased with the hardness of the Lewis acid, in correspondence with the decrease of the amount of lignin and hemicellulose in the pulp.
27295251	4	10	theme	lignin	654:659	arg1	amount					644:649	the amount	640:649	the amount of lignin and hemicellulose in the pulp	640:689	Surface area and pore volume increased with the hardness of the Lewis acid, in correspondence with the decrease of the amount of lignin and hemicellulose in the pulp.
27295251	0	11	theme	wheat	105:109	arg1	straw					111:115	wheat straw	105:115	wheat straw	105:115	Composition, texture and methane potential of cellulosic residues from Lewis acids organosolv pulping of wheat straw.
27295251	6	12	theme	methane	907:913	arg1	potential					915:923	a significantly higher methane potential	884:923	a significantly higher methane potential than the parent straw	884:945	All organosolv pulps presented a significantly higher methane potential than the parent straw.
27295251	6	13	theme	parent	934:939	arg1	straw					941:945	the parent straw	930:945	the parent straw	930:945	All organosolv pulps presented a significantly higher methane potential than the parent straw.
27295251	5	14	theme	pulping	844:850	arg1	stages					834:839	the early stages	824:839	the early stages of pulping	824:850	The non linearity of the correlation between porosity and composition suggests that an agglomeration of cellulose fibrils occurs in the early stages of pulping.
27295251	4	15	theme	hemicellulose	665:677	arg1	hemicellulose					665:677	hemicellulose	665:677	hemicellulose	665:677	Surface area and pore volume increased with the hardness of the Lewis acid, in correspondence with the decrease of the amount of lignin and hemicellulose in the pulp.
27295251	4	15	theme	hemicellulose	665:677	arg1	lignin					654:659	lignin	654:659	lignin	654:659	Surface area and pore volume increased with the hardness of the Lewis acid, in correspondence with the decrease of the amount of lignin and hemicellulose in the pulp.
27295251	4	15	theme	hemicellulose	665:677	arg1	amount					644:649	the amount	640:649	the amount of lignin and hemicellulose in the pulp	640:689	Surface area and pore volume increased with the hardness of the Lewis acid, in correspondence with the decrease of the amount of lignin and hemicellulose in the pulp.
27295251	1	16	attach	isolated	158:165	arg1	straw					178:182	wheat straw	172:182	wheat straw	172:182	Cellulosic pulps have been successfully isolated from wheat straw through a Lewis acids organosolv treatment.
27295251	1	16	attach	isolated	158:165	arg2	pulps					129:133	Cellulosic pulps	118:133	Cellulosic pulps	118:133	Cellulosic pulps have been successfully isolated from wheat straw through a Lewis acids organosolv treatment.
27295251	6	17	theme	organosolv	857:866	arg1	pulps					868:872	All organosolv pulps	853:872	All organosolv pulps	853:872	All organosolv pulps presented a significantly higher methane potential than the parent straw.
27295251	2	18	theme	different	256:264	arg1	hardness					266:273	different hardness	256:273	different hardness	256:273	The use of Lewis acids with different hardness produced pulps with different delignification degrees.
27295251	1	19	theme	wheat	172:176	arg1	straw					178:182	wheat straw	172:182	wheat straw	172:182	Cellulosic pulps have been successfully isolated from wheat straw through a Lewis acids organosolv treatment.
27295251	3	20	theme	X-ray	396:400	arg1	diffraction					402:412	X-ray diffraction	396:412	X-ray diffraction	396:412	The cellulosic residue was characterised by chemical composition, X-ray diffraction, FT-IR spectroscopy, N2 physisorption, scanning electron microscopy and potential for anaerobic digestibility.
27295251	3	21	theme	N2	435:436	arg1	physisorption					438:450	N2 physisorption	435:450	N2 physisorption	435:450	The cellulosic residue was characterised by chemical composition, X-ray diffraction, FT-IR spectroscopy, N2 physisorption, scanning electron microscopy and potential for anaerobic digestibility.
27295251	2	22	theme	delignification	305:319	arg1	degrees					321:327	different delignification degrees	295:327	different delignification degrees	295:327	The use of Lewis acids with different hardness produced pulps with different delignification degrees.
27295251	4	23	theme	Surface	525:531	arg1	area					533:536	Surface area	525:536	Surface area	525:536	Surface area and pore volume increased with the hardness of the Lewis acid, in correspondence with the decrease of the amount of lignin and hemicellulose in the pulp.
27295251	7	24	theme	native	1052:1057	arg1	straw					1059:1063	the native straw	1048:1063	the native straw	1048:1063	A methane evolution of 295Ncm(3)/g OM was reached by a moderate improvement of the accessibility of the native straw.
27295251	7	25	theme	295Ncm	971:976	arg1	OM					983:984	295Ncm(3)/g OM	971:984	295Ncm(3)/g OM	971:984	A methane evolution of 295Ncm(3)/g OM was reached by a moderate improvement of the accessibility of the native straw.
27295251	2	26	theme	different	295:303	arg1	degrees					321:327	different delignification degrees	295:327	different delignification degrees	295:327	The use of Lewis acids with different hardness produced pulps with different delignification degrees.
27295251	4	27	theme	amount	644:649	arg1	decrease					628:635	the decrease	624:635	the decrease of the amount of lignin and hemicellulose in the pulp	624:689	Surface area and pore volume increased with the hardness of the Lewis acid, in correspondence with the decrease of the amount of lignin and hemicellulose in the pulp.
27295251	2	28	with	use	232:234	arg1	hardness					266:273	different hardness	256:273	different hardness	256:273	The use of Lewis acids with different hardness produced pulps with different delignification degrees.
27295251	0	29	theme	straw	111:115	arg1	pulping					94:100	pulping	94:100	pulping of wheat straw	94:115	Composition, texture and methane potential of cellulosic residues from Lewis acids organosolv pulping of wheat straw.
27295251	0	30	from	acids	77:81	arg1	texture					13:19	texture	13:19	texture	13:19	Composition, texture and methane potential of cellulosic residues from Lewis acids organosolv pulping of wheat straw.
27295251	0	30	from	acids	77:81	arg1	potential					33:41	methane potential	25:41	methane potential	25:41	Composition, texture and methane potential of cellulosic residues from Lewis acids organosolv pulping of wheat straw.
27295251	0	30	from	acids	77:81	arg1	Composition					0:10	Composition	0:10	Composition	0:10	Composition, texture and methane potential of cellulosic residues from Lewis acids organosolv pulping of wheat straw.
27295251	4	31	from	amount	644:649	arg1	pulp					686:689	the pulp	682:689	the pulp	682:689	Surface area and pore volume increased with the hardness of the Lewis acid, in correspondence with the decrease of the amount of lignin and hemicellulose in the pulp.
27295251	1	32	theme	Lewis	194:198	arg1	acids					200:204	Lewis acids	194:204	a Lewis acids organosolv treatment	192:225	Cellulosic pulps have been successfully isolated from wheat straw through a Lewis acids organosolv treatment.
27295251	3	33	theme	electron	462:469	arg1	microscopy					471:480	scanning electron microscopy	453:480	scanning electron microscopy	453:480	The cellulosic residue was characterised by chemical composition, X-ray diffraction, FT-IR spectroscopy, N2 physisorption, scanning electron microscopy and potential for anaerobic digestibility.
27295251	5	34	theme	correlation	717:727	arg1	linearity					700:708	The non linearity	692:708	The non linearity of the correlation between porosity and composition	692:760	The non linearity of the correlation between porosity and composition suggests that an agglomeration of cellulose fibrils occurs in the early stages of pulping.
27295251	1	35	theme	acids	200:204	arg1	treatment					217:225	a Lewis acids organosolv treatment	192:225	a Lewis acids organosolv treatment	192:225	Cellulosic pulps have been successfully isolated from wheat straw through a Lewis acids organosolv treatment.
27295251	0	36	theme	methane	25:31	arg1	potential					33:41	methane potential	25:41	methane potential	25:41	Composition, texture and methane potential of cellulosic residues from Lewis acids organosolv pulping of wheat straw.
27295251	5	37	theme	cellulose	796:804	arg1	fibrils					806:812	cellulose fibrils	796:812	cellulose fibrils	796:812	The non linearity of the correlation between porosity and composition suggests that an agglomeration of cellulose fibrils occurs in the early stages of pulping.
27295251	5	38	theme	early	828:832	arg1	stages					834:839	the early stages	824:839	the early stages of pulping	824:850	The non linearity of the correlation between porosity and composition suggests that an agglomeration of cellulose fibrils occurs in the early stages of pulping.
27295251	1	39	theme	organosolv	206:215	arg1	treatment					217:225	a Lewis acids organosolv treatment	192:225	a Lewis acids organosolv treatment	192:225	Cellulosic pulps have been successfully isolated from wheat straw through a Lewis acids organosolv treatment.
27295251	0	40	theme	cellulosic	46:55	arg1	residues					57:64	cellulosic residues	46:64	cellulosic residues	46:64	Composition, texture and methane potential of cellulosic residues from Lewis acids organosolv pulping of wheat straw.
27295251	4	41	from	decrease	628:635	arg1	pulp					686:689	the pulp	682:689	the pulp	682:689	Surface area and pore volume increased with the hardness of the Lewis acid, in correspondence with the decrease of the amount of lignin and hemicellulose in the pulp.
27295251	6	42	theme	higher	900:905	arg1	potential					915:923	a significantly higher methane potential	884:923	a significantly higher methane potential than the parent straw	884:945	All organosolv pulps presented a significantly higher methane potential than the parent straw.
27295251	3	43	theme	FT-IR	415:419	arg1	spectroscopy					421:432	FT-IR spectroscopy	415:432	FT-IR spectroscopy	415:432	The cellulosic residue was characterised by chemical composition, X-ray diffraction, FT-IR spectroscopy, N2 physisorption, scanning electron microscopy and potential for anaerobic digestibility.
27295251	7	44	theme	accessibility	1031:1043	arg1	improvement					1012:1022	a moderate improvement	1001:1022	a moderate improvement of the accessibility of the native straw	1001:1063	A methane evolution of 295Ncm(3)/g OM was reached by a moderate improvement of the accessibility of the native straw.
27295251	7	45	theme	methane	950:956	arg1	evolution					958:966	A methane evolution	948:966	A methane evolution of 295Ncm(3)/g OM	948:984	A methane evolution of 295Ncm(3)/g OM was reached by a moderate improvement of the accessibility of the native straw.
27295251	4	46	with	correspondence	604:617	arg1	decrease					628:635	the decrease	624:635	the decrease of the amount of lignin and hemicellulose in the pulp	624:689	Surface area and pore volume increased with the hardness of the Lewis acid, in correspondence with the decrease of the amount of lignin and hemicellulose in the pulp.
27295251	1	47	theme	Cellulosic	118:127	arg1	pulps					129:133	Cellulosic pulps	118:133	Cellulosic pulps	118:133	Cellulosic pulps have been successfully isolated from wheat straw through a Lewis acids organosolv treatment.
27295251	5	48	theme	non	696:698	arg1	linearity					700:708	The non linearity	692:708	The non linearity of the correlation between porosity and composition	692:760	The non linearity of the correlation between porosity and composition suggests that an agglomeration of cellulose fibrils occurs in the early stages of pulping.
27295251	2	49	theme	acids	245:249	arg1	use					232:234	The use	228:234	The use of Lewis acids with different hardness	228:273	The use of Lewis acids with different hardness produced pulps with different delignification degrees.
27295251	7	50	theme	OM	983:984	arg1	evolution					958:966	A methane evolution	948:966	A methane evolution of 295Ncm(3)/g OM	948:984	A methane evolution of 295Ncm(3)/g OM was reached by a moderate improvement of the accessibility of the native straw.
27295251	3	51	theme	anaerobic	500:508	arg1	digestibility					510:522	anaerobic digestibility	500:522	anaerobic digestibility	500:522	The cellulosic residue was characterised by chemical composition, X-ray diffraction, FT-IR spectroscopy, N2 physisorption, scanning electron microscopy and potential for anaerobic digestibility.
27295251	3	52	theme	cellulosic	334:343	arg1	residue					345:351	The cellulosic residue	330:351	The cellulosic residue	330:351	The cellulosic residue was characterised by chemical composition, X-ray diffraction, FT-IR spectroscopy, N2 physisorption, scanning electron microscopy and potential for anaerobic digestibility.
27295251	2	53	theme	Lewis	239:243	arg1	acids					245:249	Lewis acids	239:249	Lewis acids	239:249	The use of Lewis acids with different hardness produced pulps with different delignification degrees.
27295251	3	54	theme	scanning	453:460	arg1	microscopy					471:480	scanning electron microscopy	453:480	scanning electron microscopy	453:480	The cellulosic residue was characterised by chemical composition, X-ray diffraction, FT-IR spectroscopy, N2 physisorption, scanning electron microscopy and potential for anaerobic digestibility.
27295251	2	55	with	pulps	284:288	arg1	degrees					321:327	different delignification degrees	295:327	different delignification degrees	295:327	The use of Lewis acids with different hardness produced pulps with different delignification degrees.
27295251	0	56	theme	residues	57:64	arg1	texture					13:19	texture	13:19	texture	13:19	Composition, texture and methane potential of cellulosic residues from Lewis acids organosolv pulping of wheat straw.
27295251	0	56	theme	residues	57:64	arg1	potential					33:41	methane potential	25:41	methane potential	25:41	Composition, texture and methane potential of cellulosic residues from Lewis acids organosolv pulping of wheat straw.
27295251	0	56	theme	residues	57:64	arg1	Composition					0:10	Composition	0:10	Composition	0:10	Composition, texture and methane potential of cellulosic residues from Lewis acids organosolv pulping of wheat straw.
24702938	0	0	theme	optimization	98:109	arg1	studies					111:117	medium optimization studies	91:117	medium optimization studies	91:117	Enhancing exopolysaccharide antioxidant formation and yield from Phellinus species through medium optimization studies.
24702938	4	1	theme	surface	684:690	arg1	models					692:697	response surface models	675:697	response surface models	675:697	Based on regression analysis of the experimental results from central composite design in response surface models, two second-order polynomial models adequately representing the relationship between these variables and EPS TEAC and yield were obtained, and the optimum concentration of these variables were determined and validated.
24702938	0	2	theme	medium	91:96	arg1	studies					111:117	medium optimization studies	91:117	medium optimization studies	91:117	Enhancing exopolysaccharide antioxidant formation and yield from Phellinus species through medium optimization studies.
24702938	4	3	theme	response	675:682	arg1	models					692:697	response surface models	675:697	response surface models	675:697	Based on regression analysis of the experimental results from central composite design in response surface models, two second-order polynomial models adequately representing the relationship between these variables and EPS TEAC and yield were obtained, and the optimum concentration of these variables were determined and validated.
24702938	1	4	theme	Phellinus	266:274	arg1	sp					276:277	Phellinus sp	266:277	Phellinus sp	266:277	The study was conducted to enhance exopolysaccharide (EPS) antioxidant formation (as measured by Trolox-Equivalent Antioxidant Capacity, TEAC) by Phellinus sp.
24702938	3	5	theme	medium	359:364	arg1	components					366:375	seven medium components	353:375	seven medium components	353:375	Out of seven medium components, four significant variables were identified based on linear models with statistical significance (p<0.05), namely rutin, FeSO4, and aspartate, which influenced TEAC, and malt extract, which influenced yield.
24702938	4	6	theme	variables	877:885	arg1	concentration					854:866	the optimum concentration	842:866	the optimum concentration of these variables	842:885	Based on regression analysis of the experimental results from central composite design in response surface models, two second-order polynomial models adequately representing the relationship between these variables and EPS TEAC and yield were obtained, and the optimum concentration of these variables were determined and validated.
24702938	6	7	theme	optimum	1141:1147	arg1	combinations					1158:1169	the optimum variable combinations	1137:1169	the optimum variable combinations	1137:1169	The maximum EPS TEAC and yield reached 8.49 ± 0.32 mM and 10.92 ± 0.68 g/L, respectively, in a 7 L bioreactor under the optimum variable combinations.
24702938	4	8	from	design	665:670	arg1	analysis					605:612	regression analysis	594:612	regression analysis of the experimental results from central composite design in response surface models	594:697	Based on regression analysis of the experimental results from central composite design in response surface models, two second-order polynomial models adequately representing the relationship between these variables and EPS TEAC and yield were obtained, and the optimum concentration of these variables were determined and validated.
24702938	4	8	from	design	665:670	arg1	results					634:640	the experimental results	617:640	the experimental results from central composite design in response surface models	617:697	Based on regression analysis of the experimental results from central composite design in response surface models, two second-order polynomial models adequately representing the relationship between these variables and EPS TEAC and yield were obtained, and the optimum concentration of these variables were determined and validated.
24702938	5	9	theme	high	944:947	arg1	TEAC					953:956	TEAC	953:956	TEAC	953:956	Variable combinations for high EPS TEAC and yield were optimized through a desirability function test.
24702938	5	9	theme	high	944:947	arg1	EPS					949:951	high EPS TEAC and yield	944:966	high EPS TEAC and yield	944:966	Variable combinations for high EPS TEAC and yield were optimized through a desirability function test.
24702938	5	9	theme	high	944:947	arg1	yield					962:966	yield	962:966	yield	962:966	Variable combinations for high EPS TEAC and yield were optimized through a desirability function test.
24702938	6	10	from	mM	1072:1073	arg1	bioreactor					1120:1129	a 7 L bioreactor	1114:1129	a 7 L bioreactor	1114:1129	The maximum EPS TEAC and yield reached 8.49 ± 0.32 mM and 10.92 ± 0.68 g/L, respectively, in a 7 L bioreactor under the optimum variable combinations.
24702938	4	11	theme	composite	655:663	arg1	design					665:670	central composite design	647:670	central composite design	647:670	Based on regression analysis of the experimental results from central composite design in response surface models, two second-order polynomial models adequately representing the relationship between these variables and EPS TEAC and yield were obtained, and the optimum concentration of these variables were determined and validated.
24702938	1	12	theme	antioxidant	179:189	arg1	formation					191:199	exopolysaccharide (EPS) antioxidant formation	155:199	exopolysaccharide (EPS) antioxidant formation (as measured by Trolox-Equivalent Antioxidant Capacity, TEAC)	155:261	The study was conducted to enhance exopolysaccharide (EPS) antioxidant formation (as measured by Trolox-Equivalent Antioxidant Capacity, TEAC) by Phellinus sp.
24702938	1	12	theme	antioxidant	179:189	arg1	measured					205:212	measured	205:212	measured by Trolox-Equivalent Antioxidant Capacity	205:254	The study was conducted to enhance exopolysaccharide (EPS) antioxidant formation (as measured by Trolox-Equivalent Antioxidant Capacity, TEAC) by Phellinus sp.
24702938	6	13	theme	maximum	1025:1031	arg1	TEAC					1037:1040	The maximum EPS TEAC	1021:1040	The maximum EPS TEAC	1021:1040	The maximum EPS TEAC and yield reached 8.49 ± 0.32 mM and 10.92 ± 0.68 g/L, respectively, in a 7 L bioreactor under the optimum variable combinations.
24702938	3	14	theme	statistical	449:459	arg1	p<0.05					475:480	p<0.05	475:480	p<0.05	475:480	Out of seven medium components, four significant variables were identified based on linear models with statistical significance (p<0.05), namely rutin, FeSO4, and aspartate, which influenced TEAC, and malt extract, which influenced yield.
24702938	3	14	theme	statistical	449:459	arg1	significance					461:472	statistical significance	449:472	statistical significance (p<0.05)	449:481	Out of seven medium components, four significant variables were identified based on linear models with statistical significance (p<0.05), namely rutin, FeSO4, and aspartate, which influenced TEAC, and malt extract, which influenced yield.
24702938	0	15	theme	exopolysaccharide	10:26	arg1	formation					40:48	exopolysaccharide antioxidant formation	10:48	exopolysaccharide antioxidant formation	10:48	Enhancing exopolysaccharide antioxidant formation and yield from Phellinus species through medium optimization studies.
24702938	6	16	theme	L	1118:1118	arg1	bioreactor					1120:1129	a 7 L bioreactor	1114:1129	a 7 L bioreactor	1114:1129	The maximum EPS TEAC and yield reached 8.49 ± 0.32 mM and 10.92 ± 0.68 g/L, respectively, in a 7 L bioreactor under the optimum variable combinations.
24702938	4	17	theme	optimum	846:852	arg1	concentration					854:866	the optimum concentration	842:866	the optimum concentration of these variables	842:885	Based on regression analysis of the experimental results from central composite design in response surface models, two second-order polynomial models adequately representing the relationship between these variables and EPS TEAC and yield were obtained, and the optimum concentration of these variables were determined and validated.
24702938	3	18	theme	malt	547:550	arg1	extract					552:558	malt extract	547:558	malt extract	547:558	Out of seven medium components, four significant variables were identified based on linear models with statistical significance (p<0.05), namely rutin, FeSO4, and aspartate, which influenced TEAC, and malt extract, which influenced yield.
24702938	4	19	from	results	634:640	arg1	models					692:697	response surface models	675:697	response surface models	675:697	Based on regression analysis of the experimental results from central composite design in response surface models, two second-order polynomial models adequately representing the relationship between these variables and EPS TEAC and yield were obtained, and the optimum concentration of these variables were determined and validated.
24702938	3	20	theme	linear	430:435	arg1	rutin					491:495	rutin	491:495	rutin	491:495	Out of seven medium components, four significant variables were identified based on linear models with statistical significance (p<0.05), namely rutin, FeSO4, and aspartate, which influenced TEAC, and malt extract, which influenced yield.
24702938	3	20	theme	linear	430:435	arg1	models					437:442	linear models	430:442	linear models with statistical significance (p<0.05)	430:481	Out of seven medium components, four significant variables were identified based on linear models with statistical significance (p<0.05), namely rutin, FeSO4, and aspartate, which influenced TEAC, and malt extract, which influenced yield.
24702938	3	20	theme	linear	430:435	arg1	FeSO4					498:502	FeSO4	498:502	FeSO4	498:502	Out of seven medium components, four significant variables were identified based on linear models with statistical significance (p<0.05), namely rutin, FeSO4, and aspartate, which influenced TEAC, and malt extract, which influenced yield.
24702938	3	20	theme	linear	430:435	arg1	aspartate					509:517	aspartate	509:517	aspartate	509:517	Out of seven medium components, four significant variables were identified based on linear models with statistical significance (p<0.05), namely rutin, FeSO4, and aspartate, which influenced TEAC, and malt extract, which influenced yield.
24702938	4	21	theme	polynomial	717:726	arg1	models					728:733	two second-order polynomial models	700:733	two second-order polynomial models adequately representing the relationship between these variables and EPS TEAC and yield	700:821	Based on regression analysis of the experimental results from central composite design in response surface models, two second-order polynomial models adequately representing the relationship between these variables and EPS TEAC and yield were obtained, and the optimum concentration of these variables were determined and validated.
24702938	4	22	theme	central	647:653	arg1	design					665:670	central composite design	647:670	central composite design	647:670	Based on regression analysis of the experimental results from central composite design in response surface models, two second-order polynomial models adequately representing the relationship between these variables and EPS TEAC and yield were obtained, and the optimum concentration of these variables were determined and validated.
24702938	5	23	theme	function	1006:1013	arg1	test					1015:1018	a desirability function test	991:1018	a desirability function test	991:1018	Variable combinations for high EPS TEAC and yield were optimized through a desirability function test.
24702938	5	24	dep	EPS	949:951	arg1	TEAC					953:956	TEAC	953:956	TEAC	953:956	Variable combinations for high EPS TEAC and yield were optimized through a desirability function test.
24702938	5	24	dep	EPS	949:951	arg1	EPS					949:951	high EPS TEAC and yield	944:966	high EPS TEAC and yield	944:966	Variable combinations for high EPS TEAC and yield were optimized through a desirability function test.
24702938	5	24	dep	EPS	949:951	arg1	yield					962:966	yield	962:966	yield	962:966	Variable combinations for high EPS TEAC and yield were optimized through a desirability function test.
24702938	0	25	theme	antioxidant	28:38	arg1	formation					40:48	exopolysaccharide antioxidant formation	10:48	exopolysaccharide antioxidant formation	10:48	Enhancing exopolysaccharide antioxidant formation and yield from Phellinus species through medium optimization studies.
24702938	4	26	from	analysis	605:612	arg1	design					665:670	central composite design	647:670	central composite design	647:670	Based on regression analysis of the experimental results from central composite design in response surface models, two second-order polynomial models adequately representing the relationship between these variables and EPS TEAC and yield were obtained, and the optimum concentration of these variables were determined and validated.
24702938	4	26	from	analysis	605:612	arg1	models					692:697	response surface models	675:697	response surface models	675:697	Based on regression analysis of the experimental results from central composite design in response surface models, two second-order polynomial models adequately representing the relationship between these variables and EPS TEAC and yield were obtained, and the optimum concentration of these variables were determined and validated.
24702938	4	27	theme	experimental	621:632	arg1	results					634:640	the experimental results	617:640	the experimental results from central composite design in response surface models	617:697	Based on regression analysis of the experimental results from central composite design in response surface models, two second-order polynomial models adequately representing the relationship between these variables and EPS TEAC and yield were obtained, and the optimum concentration of these variables were determined and validated.
24702938	4	28	from	models	692:697	arg1	analysis					605:612	regression analysis	594:612	regression analysis of the experimental results from central composite design in response surface models	594:697	Based on regression analysis of the experimental results from central composite design in response surface models, two second-order polynomial models adequately representing the relationship between these variables and EPS TEAC and yield were obtained, and the optimum concentration of these variables were determined and validated.
24702938	1	29	theme	Trolox-Equivalent	217:233	arg1	Capacity					247:254	Trolox-Equivalent Antioxidant Capacity	217:254	Trolox-Equivalent Antioxidant Capacity	217:254	The study was conducted to enhance exopolysaccharide (EPS) antioxidant formation (as measured by Trolox-Equivalent Antioxidant Capacity, TEAC) by Phellinus sp.
24702938	6	30	theme	±	1065:1065	arg1	mM					1072:1073	8.49 ± 0.32 mM	1060:1073	8.49 ± 0.32 mM	1060:1073	The maximum EPS TEAC and yield reached 8.49 ± 0.32 mM and 10.92 ± 0.68 g/L, respectively, in a 7 L bioreactor under the optimum variable combinations.
24702938	1	31	theme	Antioxidant	235:245	arg1	Capacity					247:254	Trolox-Equivalent Antioxidant Capacity	217:254	Trolox-Equivalent Antioxidant Capacity	217:254	The study was conducted to enhance exopolysaccharide (EPS) antioxidant formation (as measured by Trolox-Equivalent Antioxidant Capacity, TEAC) by Phellinus sp.
24702938	0	32	theme	Phellinus	65:73	arg1	species					75:81	Phellinus species	65:81	Phellinus species	65:81	Enhancing exopolysaccharide antioxidant formation and yield from Phellinus species through medium optimization studies.
24702938	0	33	from	species	75:81	arg1	yield					54:58	yield	54:58	yield	54:58	Enhancing exopolysaccharide antioxidant formation and yield from Phellinus species through medium optimization studies.
24702938	0	33	from	species	75:81	arg1	formation					40:48	exopolysaccharide antioxidant formation	10:48	exopolysaccharide antioxidant formation	10:48	Enhancing exopolysaccharide antioxidant formation and yield from Phellinus species through medium optimization studies.
24702938	6	34	from	g/L	1092:1094	arg1	bioreactor					1120:1129	a 7 L bioreactor	1114:1129	a 7 L bioreactor	1114:1129	The maximum EPS TEAC and yield reached 8.49 ± 0.32 mM and 10.92 ± 0.68 g/L, respectively, in a 7 L bioreactor under the optimum variable combinations.
24702938	3	35	with	models	437:442	arg1	p<0.05					475:480	p<0.05	475:480	p<0.05	475:480	Out of seven medium components, four significant variables were identified based on linear models with statistical significance (p<0.05), namely rutin, FeSO4, and aspartate, which influenced TEAC, and malt extract, which influenced yield.
24702938	3	35	with	models	437:442	arg1	significance					461:472	statistical significance	449:472	statistical significance (p<0.05)	449:481	Out of seven medium components, four significant variables were identified based on linear models with statistical significance (p<0.05), namely rutin, FeSO4, and aspartate, which influenced TEAC, and malt extract, which influenced yield.
24702938	5	36	theme	Variable	918:925	arg1	combinations					927:938	Variable combinations	918:938	Variable combinations for high EPS TEAC and yield	918:966	Variable combinations for high EPS TEAC and yield were optimized through a desirability function test.
24702938	6	37	theme	variable	1149:1156	arg1	combinations					1158:1169	the optimum variable combinations	1137:1169	the optimum variable combinations	1137:1169	The maximum EPS TEAC and yield reached 8.49 ± 0.32 mM and 10.92 ± 0.68 g/L, respectively, in a 7 L bioreactor under the optimum variable combinations.
24702938	3	38	dep	influenced	526:535	arg1	influenced					567:576	influenced	567:576	influenced yield	567:582	Out of seven medium components, four significant variables were identified based on linear models with statistical significance (p<0.05), namely rutin, FeSO4, and aspartate, which influenced TEAC, and malt extract, which influenced yield.
24702938	6	39	theme	EPS	1033:1035	arg1	TEAC					1037:1040	The maximum EPS TEAC	1021:1040	The maximum EPS TEAC	1021:1040	The maximum EPS TEAC and yield reached 8.49 ± 0.32 mM and 10.92 ± 0.68 g/L, respectively, in a 7 L bioreactor under the optimum variable combinations.
24702938	5	40	theme	desirability	993:1004	arg1	test					1015:1018	a desirability function test	991:1018	a desirability function test	991:1018	Variable combinations for high EPS TEAC and yield were optimized through a desirability function test.
24702938	2	41	theme	EPS	335:337	arg1	yield					339:343	EPS yield	335:343	EPS yield	335:343	P0988 through medium studies, while jointly increasing EPS yield.
24702938	4	42	theme	second-order	704:715	arg1	models					728:733	two second-order polynomial models	700:733	two second-order polynomial models adequately representing the relationship between these variables and EPS TEAC and yield	700:821	Based on regression analysis of the experimental results from central composite design in response surface models, two second-order polynomial models adequately representing the relationship between these variables and EPS TEAC and yield were obtained, and the optimum concentration of these variables were determined and validated.
24702938	6	43	theme	±	1085:1085	arg1	g/L					1092:1094	10.92 ± 0.68 g/L	1079:1094	10.92 ± 0.68 g/L	1079:1094	The maximum EPS TEAC and yield reached 8.49 ± 0.32 mM and 10.92 ± 0.68 g/L, respectively, in a 7 L bioreactor under the optimum variable combinations.
24702938	1	44	dep	measured	205:212	arg1	TEAC					257:260	TEAC	257:260	TEAC	257:260	The study was conducted to enhance exopolysaccharide (EPS) antioxidant formation (as measured by Trolox-Equivalent Antioxidant Capacity, TEAC) by Phellinus sp.
24702938	1	45	theme	exopolysaccharide	155:171	arg1	formation					191:199	exopolysaccharide (EPS) antioxidant formation	155:199	exopolysaccharide (EPS) antioxidant formation (as measured by Trolox-Equivalent Antioxidant Capacity, TEAC)	155:261	The study was conducted to enhance exopolysaccharide (EPS) antioxidant formation (as measured by Trolox-Equivalent Antioxidant Capacity, TEAC) by Phellinus sp.
24702938	1	45	theme	exopolysaccharide	155:171	arg1	measured					205:212	measured	205:212	measured by Trolox-Equivalent Antioxidant Capacity	205:254	The study was conducted to enhance exopolysaccharide (EPS) antioxidant formation (as measured by Trolox-Equivalent Antioxidant Capacity, TEAC) by Phellinus sp.
24702938	4	46	theme	results	634:640	arg1	analysis					605:612	regression analysis	594:612	regression analysis of the experimental results from central composite design in response surface models	594:697	Based on regression analysis of the experimental results from central composite design in response surface models, two second-order polynomial models adequately representing the relationship between these variables and EPS TEAC and yield were obtained, and the optimum concentration of these variables were determined and validated.
24702938	4	47	theme	regression	594:603	arg1	analysis					605:612	regression analysis	594:612	regression analysis of the experimental results from central composite design in response surface models	594:697	Based on regression analysis of the experimental results from central composite design in response surface models, two second-order polynomial models adequately representing the relationship between these variables and EPS TEAC and yield were obtained, and the optimum concentration of these variables were determined and validated.
24702938	4	48	theme	EPS	804:806	arg1	TEAC					808:811	EPS TEAC	804:811	EPS TEAC	804:811	Based on regression analysis of the experimental results from central composite design in response surface models, two second-order polynomial models adequately representing the relationship between these variables and EPS TEAC and yield were obtained, and the optimum concentration of these variables were determined and validated.
24702938	3	49	theme	significant	383:393	arg1	variables					395:403	four significant variables	378:403	four significant variables	378:403	Out of seven medium components, four significant variables were identified based on linear models with statistical significance (p<0.05), namely rutin, FeSO4, and aspartate, which influenced TEAC, and malt extract, which influenced yield.
25909978	5	0	theme	forest	668:673	arg1	harvesting					675:684	forest harvesting	668:684	forest harvesting	668:684	The subsequent metagenome analysis revealed that, 12 years after forest harvesting, there were 16% and 8% reductions in relative abundances of biomass decomposition genes in the organic and mineral soil layers, respectively.
25909978	1	1	theme	sustainable	140:150	arg1	productivity					159:170	sustainable forest productivity	140:170	sustainable forest productivity	140:170	Soil is the key resource that must be managed to ensure sustainable forest productivity.
25909978	5	2	from	reductions	709:718	arg1	abundances					732:741	relative abundances	723:741	relative abundances of biomass decomposition genes	723:772	The subsequent metagenome analysis revealed that, 12 years after forest harvesting, there were 16% and 8% reductions in relative abundances of biomass decomposition genes in the organic and mineral soil layers, respectively.
25909978	3	3	theme	long-term	337:345	arg1	site					371:374	a long-term soil productivity study site	335:374	a long-term soil productivity study site in a temperate coniferous forest in British Columbia	335:427	From a long-term soil productivity study site in a temperate coniferous forest in British Columbia, 21 forest soil shotgun metagenomes were generated, totaling 187 Gb.
25909978	5	4	theme	metagenome	618:627	arg1	analysis					629:636	The subsequent metagenome analysis	603:636	The subsequent metagenome analysis	603:636	The subsequent metagenome analysis revealed that, 12 years after forest harvesting, there were 16% and 8% reductions in relative abundances of biomass decomposition genes in the organic and mineral soil layers, respectively.
25909978	1	5	theme	forest	152:157	arg1	productivity					159:170	sustainable forest productivity	140:170	sustainable forest productivity	140:170	Soil is the key resource that must be managed to ensure sustainable forest productivity.
25909978	5	6	theme	relative	723:730	arg1	abundances					732:741	relative abundances	723:741	relative abundances of biomass decomposition genes	723:772	The subsequent metagenome analysis revealed that, 12 years after forest harvesting, there were 16% and 8% reductions in relative abundances of biomass decomposition genes in the organic and mineral soil layers, respectively.
25909978	4	7	theme	unassembled	518:528	arg1	metagenome					530:539	unassembled metagenome reads	518:545	unassembled metagenome reads from the complex community	518:572	A method to analyze unassembled metagenome reads from the complex community was optimized and validated.
25909978	2	8	theme	soil	302:305	arg1	composition					317:327	soil community composition	302:327	soil community composition	302:327	Soil microbial communities mediate numerous essential ecosystem functions, and recent studies show that forest harvesting alters soil community composition.
25909978	6	9	theme	biomass	903:909	arg1	degradation					911:921	biomass degradation	903:921	biomass degradation	903:921	Organic and mineral soil layers differed markedly in genetic potential for biomass degradation, with the organic layer having greater potential and being more strongly affected by harvesting.
25909978	9	10	theme	forests	1611:1617	arg1	characteristic					1583:1596	characteristic	1583:1596	characteristic	1583:1596	Thus, it is important to determine whether these changes foreshadow long-term changes in forest productivity or resilience and whether these changes are broadly characteristic of harvested forests.
25909978	8	11	theme	important	1374:1382	arg1	consequences					1384:1395	potentially important consequences	1362:1395	potentially important consequences for forest regeneration	1362:1419	The results strongly suggest that harvesting profoundly altered below-ground cycling of carbon and other nutrients at this site, with potentially important consequences for forest regeneration.
25909978	3	12	theme	study	365:369	arg1	site					371:374	a long-term soil productivity study site	335:374	a long-term soil productivity study site in a temperate coniferous forest in British Columbia	335:427	From a long-term soil productivity study site in a temperate coniferous forest in British Columbia, 21 forest soil shotgun metagenomes were generated, totaling 187 Gb.
25909978	5	13	dep	years	656:660	arg1	harvesting					675:684	forest harvesting	668:684	forest harvesting	668:684	The subsequent metagenome analysis revealed that, 12 years after forest harvesting, there were 16% and 8% reductions in relative abundances of biomass decomposition genes in the organic and mineral soil layers, respectively.
25909978	8	14	theme	below-ground	1292:1303	arg1	cycling					1305:1311	below-ground cycling	1292:1311	below-ground cycling of carbon and other nutrients	1292:1341	The results strongly suggest that harvesting profoundly altered below-ground cycling of carbon and other nutrients at this site, with potentially important consequences for forest regeneration.
25909978	3	15	theme	shotgun	445:451	arg1	metagenomes					453:463	21 forest soil shotgun metagenomes	430:463	21 forest soil shotgun metagenomes	430:463	From a long-term soil productivity study site in a temperate coniferous forest in British Columbia, 21 forest soil shotgun metagenomes were generated, totaling 187 Gb.
25909978	5	16	theme	biomass	746:752	arg1	genes					768:772	biomass decomposition genes	746:772	biomass decomposition genes	746:772	The subsequent metagenome analysis revealed that, 12 years after forest harvesting, there were 16% and 8% reductions in relative abundances of biomass decomposition genes in the organic and mineral soil layers, respectively.
25909978	3	17	from	forest	402:407	arg1	Columbia					420:427	Columbia	420:427	Columbia	420:427	From a long-term soil productivity study site in a temperate coniferous forest in British Columbia, 21 forest soil shotgun metagenomes were generated, totaling 187 Gb.
25909978	5	18	theme	organic	781:787	arg1	layers					806:811	the organic and mineral soil layers	777:811	the organic and mineral soil layers	777:811	The subsequent metagenome analysis revealed that, 12 years after forest harvesting, there were 16% and 8% reductions in relative abundances of biomass decomposition genes in the organic and mineral soil layers, respectively.
25909978	2	19	theme	forest	277:282	arg1	harvesting					284:293	forest harvesting	277:293	forest harvesting	277:293	Soil microbial communities mediate numerous essential ecosystem functions, and recent studies show that forest harvesting alters soil community composition.
25909978	9	20	theme	long-term	1490:1498	arg1	changes					1500:1506	long-term changes	1490:1506	long-term changes in forest productivity or resilience	1490:1543	Thus, it is important to determine whether these changes foreshadow long-term changes in forest productivity or resilience and whether these changes are broadly characteristic of harvested forests.
25909978	6	21	theme	genetic	881:887	arg1	potential					889:897	genetic potential	881:897	genetic potential for biomass degradation	881:921	Organic and mineral soil layers differed markedly in genetic potential for biomass degradation, with the organic layer having greater potential and being more strongly affected by harvesting.
25909978	7	22	dep	lignin	1171:1176	arg1	degradation					1215:1225	degradation	1215:1225	degradation	1215:1225	Gene families were disproportionately affected, and we identified 41 gene families consistently affected by harvesting, including families involved in lignin, cellulose, hemicellulose and pectin degradation.
25909978	5	23	theme	decomposition	754:766	arg1	genes					768:772	biomass decomposition genes	746:772	biomass decomposition genes	746:772	The subsequent metagenome analysis revealed that, 12 years after forest harvesting, there were 16% and 8% reductions in relative abundances of biomass decomposition genes in the organic and mineral soil layers, respectively.
25909978	3	24	theme	forest	433:438	arg1	soil					440:443	21 forest soil	430:443	21 forest soil shotgun metagenomes	430:463	From a long-term soil productivity study site in a temperate coniferous forest in British Columbia, 21 forest soil shotgun metagenomes were generated, totaling 187 Gb.
25909978	0	25	theme	Forest	0:5	arg1	harvesting					7:16	Forest harvesting	0:16	Forest harvesting	0:16	Forest harvesting reduces the soil metagenomic potential for biomass decomposition.
25909978	4	26	dep	method	500:505	arg1	analyze					510:516	analyze	510:516	to analyze unassembled metagenome reads from the complex community	507:572	A method to analyze unassembled metagenome reads from the complex community was optimized and validated.
25909978	6	27	theme	soil	848:851	arg1	layers					853:858	Organic and mineral soil layers	828:858	Organic and mineral soil layers	828:858	Organic and mineral soil layers differed markedly in genetic potential for biomass degradation, with the organic layer having greater potential and being more strongly affected by harvesting.
25909978	8	28	theme	forest	1401:1406	arg1	regeneration					1408:1419	forest regeneration	1401:1419	forest regeneration	1401:1419	The results strongly suggest that harvesting profoundly altered below-ground cycling of carbon and other nutrients at this site, with potentially important consequences for forest regeneration.
25909978	3	29	theme	soil	440:443	arg1	metagenomes					453:463	21 forest soil shotgun metagenomes	430:463	21 forest soil shotgun metagenomes	430:463	From a long-term soil productivity study site in a temperate coniferous forest in British Columbia, 21 forest soil shotgun metagenomes were generated, totaling 187 Gb.
25909978	3	30	theme	temperate	381:389	arg1	forest					402:407	a temperate coniferous forest	379:407	a temperate coniferous forest in British Columbia	379:427	From a long-term soil productivity study site in a temperate coniferous forest in British Columbia, 21 forest soil shotgun metagenomes were generated, totaling 187 Gb.
25909978	5	31	theme	subsequent	607:616	arg1	analysis					629:636	The subsequent metagenome analysis	603:636	The subsequent metagenome analysis	603:636	The subsequent metagenome analysis revealed that, 12 years after forest harvesting, there were 16% and 8% reductions in relative abundances of biomass decomposition genes in the organic and mineral soil layers, respectively.
25909978	1	32	theme	key	96:98	arg1	Soil					84:87	Soil	84:87	Soil	84:87	Soil is the key resource that must be managed to ensure sustainable forest productivity.
25909978	1	32	theme	key	96:98	arg1	resource					100:107	the key resource	92:107	the key resource that must be managed to ensure sustainable forest productivity	92:170	Soil is the key resource that must be managed to ensure sustainable forest productivity.
25909978	9	33	theme	forest	1511:1516	arg1	productivity					1518:1529	forest productivity	1511:1529	forest productivity	1511:1529	Thus, it is important to determine whether these changes foreshadow long-term changes in forest productivity or resilience and whether these changes are broadly characteristic of harvested forests.
25909978	3	34	theme	coniferous	391:400	arg1	forest					402:407	a temperate coniferous forest	379:407	a temperate coniferous forest in British Columbia	379:427	From a long-term soil productivity study site in a temperate coniferous forest in British Columbia, 21 forest soil shotgun metagenomes were generated, totaling 187 Gb.
25909978	7	35	theme	gene	1089:1092	arg1	families					1094:1101	41 gene families	1086:1101	41 gene families	1086:1101	Gene families were disproportionately affected, and we identified 41 gene families consistently affected by harvesting, including families involved in lignin, cellulose, hemicellulose and pectin degradation.
25909978	7	35	theme	gene	1089:1092	arg1	families					1150:1157	families	1150:1157	families involved in lignin, cellulose, hemicellulose and pectin degradation	1150:1225	Gene families were disproportionately affected, and we identified 41 gene families consistently affected by harvesting, including families involved in lignin, cellulose, hemicellulose and pectin degradation.
25909978	2	36	theme	microbial	178:186	arg1	communities					188:198	Soil microbial communities	173:198	Soil microbial communities	173:198	Soil microbial communities mediate numerous essential ecosystem functions, and recent studies show that forest harvesting alters soil community composition.
25909978	2	37	theme	community	307:315	arg1	composition					317:327	soil community composition	302:327	soil community composition	302:327	Soil microbial communities mediate numerous essential ecosystem functions, and recent studies show that forest harvesting alters soil community composition.
25909978	0	38	theme	metagenomic	35:45	arg1	potential					47:55	the soil metagenomic potential	26:55	the soil metagenomic potential for biomass decomposition	26:81	Forest harvesting reduces the soil metagenomic potential for biomass decomposition.
25909978	2	39	theme	Soil	173:176	arg1	communities					188:198	Soil microbial communities	173:198	Soil microbial communities	173:198	Soil microbial communities mediate numerous essential ecosystem functions, and recent studies show that forest harvesting alters soil community composition.
25909978	0	40	theme	soil	30:33	arg1	potential					47:55	the soil metagenomic potential	26:55	the soil metagenomic potential for biomass decomposition	26:81	Forest harvesting reduces the soil metagenomic potential for biomass decomposition.
25909978	4	41	dep	metagenome	530:539	arg1	reads					541:545	reads	541:545	reads	541:545	A method to analyze unassembled metagenome reads from the complex community was optimized and validated.
25909978	8	42	theme	carbon	1316:1321	arg1	cycling					1305:1311	below-ground cycling	1292:1311	below-ground cycling of carbon and other nutrients	1292:1341	The results strongly suggest that harvesting profoundly altered below-ground cycling of carbon and other nutrients at this site, with potentially important consequences for forest regeneration.
25909978	5	43	theme	mineral	793:799	arg1	layers					806:811	the organic and mineral soil layers	777:811	the organic and mineral soil layers	777:811	The subsequent metagenome analysis revealed that, 12 years after forest harvesting, there were 16% and 8% reductions in relative abundances of biomass decomposition genes in the organic and mineral soil layers, respectively.
25909978	5	44	dep	%	700:700	arg1	reductions					709:718	reductions	709:718	reductions in relative abundances of biomass decomposition genes	709:772	The subsequent metagenome analysis revealed that, 12 years after forest harvesting, there were 16% and 8% reductions in relative abundances of biomass decomposition genes in the organic and mineral soil layers, respectively.
25909978	0	45	theme	biomass	61:67	arg1	decomposition					69:81	biomass decomposition	61:81	biomass decomposition	61:81	Forest harvesting reduces the soil metagenomic potential for biomass decomposition.
25909978	8	46	theme	nutrients	1333:1341	arg1	cycling					1305:1311	below-ground cycling	1292:1311	below-ground cycling of carbon and other nutrients	1292:1341	The results strongly suggest that harvesting profoundly altered below-ground cycling of carbon and other nutrients at this site, with potentially important consequences for forest regeneration.
25909978	5	47	theme	soil	801:804	arg1	layers					806:811	the organic and mineral soil layers	777:811	the organic and mineral soil layers	777:811	The subsequent metagenome analysis revealed that, 12 years after forest harvesting, there were 16% and 8% reductions in relative abundances of biomass decomposition genes in the organic and mineral soil layers, respectively.
25909978	4	48	theme	complex	556:562	arg1	community					564:572	the complex community	552:572	the complex community	552:572	A method to analyze unassembled metagenome reads from the complex community was optimized and validated.
25909978	2	49	theme	essential	217:225	arg1	functions					237:245	numerous essential ecosystem functions	208:245	numerous essential ecosystem functions	208:245	Soil microbial communities mediate numerous essential ecosystem functions, and recent studies show that forest harvesting alters soil community composition.
25909978	2	50	theme	ecosystem	227:235	arg1	functions					237:245	numerous essential ecosystem functions	208:245	numerous essential ecosystem functions	208:245	Soil microbial communities mediate numerous essential ecosystem functions, and recent studies show that forest harvesting alters soil community composition.
25909978	8	51	theme	other	1327:1331	arg1	nutrients					1333:1341	other nutrients	1327:1341	other nutrients	1327:1341	The results strongly suggest that harvesting profoundly altered below-ground cycling of carbon and other nutrients at this site, with potentially important consequences for forest regeneration.
25909978	6	52	theme	organic	933:939	arg1	layer					941:945	the organic layer	929:945	the organic layer	929:945	Organic and mineral soil layers differed markedly in genetic potential for biomass degradation, with the organic layer having greater potential and being more strongly affected by harvesting.
25909978	6	53	theme	greater	954:960	arg1	potential					962:970	greater potential	954:970	greater potential	954:970	Organic and mineral soil layers differed markedly in genetic potential for biomass degradation, with the organic layer having greater potential and being more strongly affected by harvesting.
25909978	2	54	theme	numerous	208:215	arg1	functions					237:245	numerous essential ecosystem functions	208:245	numerous essential ecosystem functions	208:245	Soil microbial communities mediate numerous essential ecosystem functions, and recent studies show that forest harvesting alters soil community composition.
25909978	5	55	theme	genes	768:772	arg1	abundances					732:741	relative abundances	723:741	relative abundances of biomass decomposition genes	723:772	The subsequent metagenome analysis revealed that, 12 years after forest harvesting, there were 16% and 8% reductions in relative abundances of biomass decomposition genes in the organic and mineral soil layers, respectively.
25909978	6	56	theme	mineral	840:846	arg1	layers					853:858	Organic and mineral soil layers	828:858	Organic and mineral soil layers	828:858	Organic and mineral soil layers differed markedly in genetic potential for biomass degradation, with the organic layer having greater potential and being more strongly affected by harvesting.
25909978	7	57	theme	Gene	1020:1023	arg1	families					1025:1032	Gene families	1020:1032	Gene families	1020:1032	Gene families were disproportionately affected, and we identified 41 gene families consistently affected by harvesting, including families involved in lignin, cellulose, hemicellulose and pectin degradation.
25909978	9	58	theme	harvested	1601:1609	arg1	forests					1611:1617	harvested forests	1601:1617	harvested forests	1601:1617	Thus, it is important to determine whether these changes foreshadow long-term changes in forest productivity or resilience and whether these changes are broadly characteristic of harvested forests.
25909978	4	59	from	community	564:572	arg1	metagenome					530:539	unassembled metagenome reads	518:545	unassembled metagenome reads from the complex community	518:572	A method to analyze unassembled metagenome reads from the complex community was optimized and validated.
25909978	9	60	from	changes	1500:1506	arg1	productivity					1518:1529	forest productivity	1511:1529	forest productivity	1511:1529	Thus, it is important to determine whether these changes foreshadow long-term changes in forest productivity or resilience and whether these changes are broadly characteristic of harvested forests.
25909978	9	60	from	changes	1500:1506	arg1	resilience					1534:1543	resilience	1534:1543	resilience	1534:1543	Thus, it is important to determine whether these changes foreshadow long-term changes in forest productivity or resilience and whether these changes are broadly characteristic of harvested forests.
25909978	3	61	theme	soil	347:350	arg1	site					371:374	a long-term soil productivity study site	335:374	a long-term soil productivity study site in a temperate coniferous forest in British Columbia	335:427	From a long-term soil productivity study site in a temperate coniferous forest in British Columbia, 21 forest soil shotgun metagenomes were generated, totaling 187 Gb.
25909978	6	62	theme	Organic	828:834	arg1	layers					853:858	Organic and mineral soil layers	828:858	Organic and mineral soil layers	828:858	Organic and mineral soil layers differed markedly in genetic potential for biomass degradation, with the organic layer having greater potential and being more strongly affected by harvesting.
25909978	3	63	theme	productivity	352:363	arg1	site					371:374	a long-term soil productivity study site	335:374	a long-term soil productivity study site in a temperate coniferous forest in British Columbia	335:427	From a long-term soil productivity study site in a temperate coniferous forest in British Columbia, 21 forest soil shotgun metagenomes were generated, totaling 187 Gb.
25909978	2	64	theme	recent	252:257	arg1	studies					259:265	recent studies	252:265	recent studies	252:265	Soil microbial communities mediate numerous essential ecosystem functions, and recent studies show that forest harvesting alters soil community composition.
25909978	3	65	from	site	371:374	arg1	forest					402:407	a temperate coniferous forest	379:407	a temperate coniferous forest in British Columbia	379:427	From a long-term soil productivity study site in a temperate coniferous forest in British Columbia, 21 forest soil shotgun metagenomes were generated, totaling 187 Gb.
26572340	2	0	theme	wall	558:561	arg1	formation					563:571	woody-cell wall formation	547:571	woody-cell wall formation	547:571	To the best of our knowledge, this is the first time that cross-linked galactoglucomannan-lignin networks have been used for the potential development of composite films inspired by woody-cell wall formation.
26572340	5	1	from	increase	873:880	arg1	cellulose					902:910	microfibrillated cellulose	885:910	microfibrillated cellulose	885:910	In general, an increase in microfibrillated cellulose resulted in a film with better thermal, mechanical and oxygen barrier performance.
26572340	1	2	theme	wood-inspired	162:174	arg1	films					176:180	new wood-inspired films	158:180	new wood-inspired films	158:180	In this study, new wood-inspired films were developed from microfibrillated cellulose and galactoglucomannan-lignin networks isolated from chemothermomechanical pulping side streams and cross-linked using laccase enzymes.
26572340	1	3	theme	side	312:315	arg1	streams					317:323	chemothermomechanical pulping side streams	282:323	chemothermomechanical pulping side streams	282:323	In this study, new wood-inspired films were developed from microfibrillated cellulose and galactoglucomannan-lignin networks isolated from chemothermomechanical pulping side streams and cross-linked using laccase enzymes.
26572340	2	4	theme	woody-cell	547:556	arg1	formation					563:571	woody-cell wall formation	547:571	woody-cell wall formation	547:571	To the best of our knowledge, this is the first time that cross-linked galactoglucomannan-lignin networks have been used for the potential development of composite films inspired by woody-cell wall formation.
26572340	0	5	link	cross-linked	28:39	arg1	galactoglucomannan					41:58	cross-linked galactoglucomannan	28:58	cross-linked galactoglucomannan	28:58	Bioinspired composites from cross-linked galactoglucomannan and microfibrillated cellulose: Thermal, mechanical and oxygen barrier properties.
26572340	6	6	theme	oxygen	1076:1081	arg1	properties					1091:1100	oxygen barrier properties	1076:1100	oxygen barrier properties	1076:1100	However, the presence of glycerol decreased the thermal stability, stiffness and oxygen barrier properties of the films but improved their elongation.
26572340	0	7	theme	oxygen	116:121	arg1	properties					131:140	oxygen barrier properties	116:140	oxygen barrier properties	116:140	Bioinspired composites from cross-linked galactoglucomannan and microfibrillated cellulose: Thermal, mechanical and oxygen barrier properties.
26572340	5	8	theme	oxygen	967:972	arg1	performance					982:992	better thermal, mechanical and oxygen barrier performance	936:992	better thermal, mechanical and oxygen barrier performance	936:992	In general, an increase in microfibrillated cellulose resulted in a film with better thermal, mechanical and oxygen barrier performance.
26572340	4	9	theme	film	825:828	arg1	performances					830:841	the film performances	821:841	the film performances	821:841	The addition of different amounts of microfibrillated cellulose as a reinforcing agent and glycerol as a plasticizer on the film performances was evaluated.
26572340	0	10	from	cellulose	81:89	arg1	composites					12:21	Bioinspired composites	0:21	Bioinspired composites from cross-linked galactoglucomannan and microfibrillated cellulose: Thermal, mechanical and oxygen barrier properties.	0:141	Bioinspired composites from cross-linked galactoglucomannan and microfibrillated cellulose: Thermal, mechanical and oxygen barrier properties.
26572340	7	11	theme	film	1191:1194	arg1	properties					1196:1205	the film properties	1187:1205	the film properties	1187:1205	Therefore, depending on the application, the film properties can be tailored by adjusting the amounts of reinforcing agent and plasticizer in the film formulation.
26572340	5	12	theme	barrier	974:980	arg1	performance					982:992	better thermal, mechanical and oxygen barrier performance	936:992	better thermal, mechanical and oxygen barrier performance	936:992	In general, an increase in microfibrillated cellulose resulted in a film with better thermal, mechanical and oxygen barrier performance.
26572340	2	13	theme	potential	494:502	arg1	development					504:514	the potential development	490:514	the potential development of composite films inspired by woody-cell wall formation	490:571	To the best of our knowledge, this is the first time that cross-linked galactoglucomannan-lignin networks have been used for the potential development of composite films inspired by woody-cell wall formation.
26572340	2	14	dep	time	413:416	arg1	used					481:484	used	481:484	have been used for the potential development of composite films inspired by woody-cell wall formation	471:571	To the best of our knowledge, this is the first time that cross-linked galactoglucomannan-lignin networks have been used for the potential development of composite films inspired by woody-cell wall formation.
26572340	4	15	theme	microfibrillated	738:753	arg1	cellulose					755:763	microfibrillated cellulose	738:763	microfibrillated cellulose as a reinforcing agent and glycerol as a plasticizer on the film performances	738:841	The addition of different amounts of microfibrillated cellulose as a reinforcing agent and glycerol as a plasticizer on the film performances was evaluated.
26572340	0	16	theme	barrier	123:129	arg1	properties					131:140	oxygen barrier properties	116:140	oxygen barrier properties	116:140	Bioinspired composites from cross-linked galactoglucomannan and microfibrillated cellulose: Thermal, mechanical and oxygen barrier properties.
26572340	6	17	theme	barrier	1083:1089	arg1	properties					1091:1100	oxygen barrier properties	1076:1100	oxygen barrier properties	1076:1100	However, the presence of glycerol decreased the thermal stability, stiffness and oxygen barrier properties of the films but improved their elongation.
26572340	5	18	theme	microfibrillated	885:900	arg1	cellulose					902:910	microfibrillated cellulose	885:910	microfibrillated cellulose	885:910	In general, an increase in microfibrillated cellulose resulted in a film with better thermal, mechanical and oxygen barrier performance.
26572340	2	19	theme	galactoglucomannan-lignin	436:460	arg1	networks					462:469	cross-linked galactoglucomannan-lignin networks	423:469	cross-linked galactoglucomannan-lignin networks	423:469	To the best of our knowledge, this is the first time that cross-linked galactoglucomannan-lignin networks have been used for the potential development of composite films inspired by woody-cell wall formation.
26572340	0	20	from	galactoglucomannan	41:58	arg1	composites					12:21	Bioinspired composites	0:21	Bioinspired composites from cross-linked galactoglucomannan and microfibrillated cellulose: Thermal, mechanical and oxygen barrier properties.	0:141	Bioinspired composites from cross-linked galactoglucomannan and microfibrillated cellulose: Thermal, mechanical and oxygen barrier properties.
26572340	7	21	theme	film	1292:1295	arg1	formulation					1297:1307	the film formulation	1288:1307	the film formulation	1288:1307	Therefore, depending on the application, the film properties can be tailored by adjusting the amounts of reinforcing agent and plasticizer in the film formulation.
26572340	2	22	theme	cross-linked	423:434	arg1	networks					462:469	cross-linked galactoglucomannan-lignin networks	423:469	cross-linked galactoglucomannan-lignin networks	423:469	To the best of our knowledge, this is the first time that cross-linked galactoglucomannan-lignin networks have been used for the potential development of composite films inspired by woody-cell wall formation.
26572340	0	23	theme	Bioinspired	0:10	arg1	composites					12:21	Bioinspired composites	0:21	Bioinspired composites from cross-linked galactoglucomannan and microfibrillated cellulose: Thermal, mechanical and oxygen barrier properties.	0:141	Bioinspired composites from cross-linked galactoglucomannan and microfibrillated cellulose: Thermal, mechanical and oxygen barrier properties.
26572340	3	24	theme	structural	644:653	arg1	analyses					691:698	thermal, structural, mechanical and oxygen permeability analyses	635:698	thermal, structural, mechanical and oxygen permeability analyses	635:698	Their capability as polymeric matrices was assessed based on thermal, structural, mechanical and oxygen permeability analyses.
26572340	1	25	theme	microfibrillated	202:217	arg1	cellulose					219:227	microfibrillated cellulose and galactoglucomannan-lignin networks	202:266	cellulose	219:227	In this study, new wood-inspired films were developed from microfibrillated cellulose and galactoglucomannan-lignin networks isolated from chemothermomechanical pulping side streams and cross-linked using laccase enzymes.
26572340	0	26	theme	cross-linked	28:39	arg1	galactoglucomannan					41:58	cross-linked galactoglucomannan	28:58	cross-linked galactoglucomannan	28:58	Bioinspired composites from cross-linked galactoglucomannan and microfibrillated cellulose: Thermal, mechanical and oxygen barrier properties.
26572340	1	27	theme	laccase	348:354	arg1	enzymes					356:362	laccase enzymes	348:362	laccase enzymes	348:362	In this study, new wood-inspired films were developed from microfibrillated cellulose and galactoglucomannan-lignin networks isolated from chemothermomechanical pulping side streams and cross-linked using laccase enzymes.
26572340	5	28	with	film	926:929	arg1	performance					982:992	better thermal, mechanical and oxygen barrier performance	936:992	better thermal, mechanical and oxygen barrier performance	936:992	In general, an increase in microfibrillated cellulose resulted in a film with better thermal, mechanical and oxygen barrier performance.
26572340	0	29	dep	Thermal	92:98	arg1	mechanical					101:110	mechanical	101:110	mechanical	101:110	Bioinspired composites from cross-linked galactoglucomannan and microfibrillated cellulose: Thermal, mechanical and oxygen barrier properties.
26572340	3	30	dep	permeability	678:689	arg1	oxygen					671:676	oxygen	671:676	oxygen	671:676	Their capability as polymeric matrices was assessed based on thermal, structural, mechanical and oxygen permeability analyses.
26572340	3	31	theme	polymeric	594:602	arg1	matrices					604:611	polymeric matrices	594:611	polymeric matrices	594:611	Their capability as polymeric matrices was assessed based on thermal, structural, mechanical and oxygen permeability analyses.
26572340	6	32	theme	thermal	1043:1049	arg1	stability					1051:1059	the thermal stability	1039:1059	the thermal stability	1039:1059	However, the presence of glycerol decreased the thermal stability, stiffness and oxygen barrier properties of the films but improved their elongation.
26572340	2	33	link	cross-linked	423:434	arg1	networks					462:469	cross-linked galactoglucomannan-lignin networks	423:469	cross-linked galactoglucomannan-lignin networks	423:469	To the best of our knowledge, this is the first time that cross-linked galactoglucomannan-lignin networks have been used for the potential development of composite films inspired by woody-cell wall formation.
26572340	3	34	theme	thermal	635:641	arg1	analyses					691:698	thermal, structural, mechanical and oxygen permeability analyses	635:698	thermal, structural, mechanical and oxygen permeability analyses	635:698	Their capability as polymeric matrices was assessed based on thermal, structural, mechanical and oxygen permeability analyses.
26572340	4	35	theme	amounts	727:733	arg1	addition					705:712	The addition	701:712	The addition of different amounts of microfibrillated cellulose as a reinforcing agent and glycerol as a plasticizer on the film performances	701:841	The addition of different amounts of microfibrillated cellulose as a reinforcing agent and glycerol as a plasticizer on the film performances was evaluated.
26572340	4	36	theme	cellulose	755:763	arg1	amounts					727:733	different amounts	717:733	different amounts of microfibrillated cellulose as a reinforcing agent and glycerol as a plasticizer on the film performances	717:841	The addition of different amounts of microfibrillated cellulose as a reinforcing agent and glycerol as a plasticizer on the film performances was evaluated.
26572340	4	36	theme	cellulose	755:763	arg1	cellulose					755:763	microfibrillated cellulose	738:763	microfibrillated cellulose as a reinforcing agent and glycerol as a plasticizer on the film performances	738:841	The addition of different amounts of microfibrillated cellulose as a reinforcing agent and glycerol as a plasticizer on the film performances was evaluated.
26572340	1	37	theme	galactoglucomannan-lignin	233:257	arg1	networks					259:266	microfibrillated cellulose and galactoglucomannan-lignin networks	202:266	networks	259:266	In this study, new wood-inspired films were developed from microfibrillated cellulose and galactoglucomannan-lignin networks isolated from chemothermomechanical pulping side streams and cross-linked using laccase enzymes.
26572340	7	38	from	plasticizer	1273:1283	arg1	formulation					1297:1307	the film formulation	1288:1307	the film formulation	1288:1307	Therefore, depending on the application, the film properties can be tailored by adjusting the amounts of reinforcing agent and plasticizer in the film formulation.
26572340	1	39	attach	isolated	268:275	arg1	streams					317:323	chemothermomechanical pulping side streams	282:323	chemothermomechanical pulping side streams	282:323	In this study, new wood-inspired films were developed from microfibrillated cellulose and galactoglucomannan-lignin networks isolated from chemothermomechanical pulping side streams and cross-linked using laccase enzymes.
26572340	1	39	attach	isolated	268:275	arg2	cellulose					219:227	microfibrillated cellulose and galactoglucomannan-lignin networks	202:266	cellulose	219:227	In this study, new wood-inspired films were developed from microfibrillated cellulose and galactoglucomannan-lignin networks isolated from chemothermomechanical pulping side streams and cross-linked using laccase enzymes.
26572340	4	40	theme	different	717:725	arg1	amounts					727:733	different amounts	717:733	different amounts of microfibrillated cellulose as a reinforcing agent and glycerol as a plasticizer on the film performances	717:841	The addition of different amounts of microfibrillated cellulose as a reinforcing agent and glycerol as a plasticizer on the film performances was evaluated.
26572340	4	40	theme	different	717:725	arg1	cellulose					755:763	microfibrillated cellulose	738:763	microfibrillated cellulose as a reinforcing agent and glycerol as a plasticizer on the film performances	738:841	The addition of different amounts of microfibrillated cellulose as a reinforcing agent and glycerol as a plasticizer on the film performances was evaluated.
26572340	2	41	theme	films	529:533	arg1	development					504:514	the potential development	490:514	the potential development of composite films inspired by woody-cell wall formation	490:571	To the best of our knowledge, this is the first time that cross-linked galactoglucomannan-lignin networks have been used for the potential development of composite films inspired by woody-cell wall formation.
26572340	6	42	theme	glycerol	1020:1027	arg1	presence					1008:1015	the presence	1004:1015	the presence of glycerol	1004:1027	However, the presence of glycerol decreased the thermal stability, stiffness and oxygen barrier properties of the films but improved their elongation.
26572340	0	43	theme	microfibrillated	64:79	arg1	cellulose					81:89	microfibrillated cellulose	64:89	microfibrillated cellulose	64:89	Bioinspired composites from cross-linked galactoglucomannan and microfibrillated cellulose: Thermal, mechanical and oxygen barrier properties.
26572340	2	44	theme	composite	519:527	arg1	films					529:533	composite films	519:533	composite films inspired by woody-cell wall formation	519:571	To the best of our knowledge, this is the first time that cross-linked galactoglucomannan-lignin networks have been used for the potential development of composite films inspired by woody-cell wall formation.
26572340	3	45	theme	mechanical	656:665	arg1	analyses					691:698	thermal, structural, mechanical and oxygen permeability analyses	635:698	thermal, structural, mechanical and oxygen permeability analyses	635:698	Their capability as polymeric matrices was assessed based on thermal, structural, mechanical and oxygen permeability analyses.
26572340	2	46	used	used	481:484	arg2	networks					462:469	cross-linked galactoglucomannan-lignin networks	423:469	cross-linked galactoglucomannan-lignin networks	423:469	To the best of our knowledge, this is the first time that cross-linked galactoglucomannan-lignin networks have been used for the potential development of composite films inspired by woody-cell wall formation.
26572340	4	47	from	plasticizer	806:816	arg1	performances					830:841	the film performances	821:841	the film performances	821:841	The addition of different amounts of microfibrillated cellulose as a reinforcing agent and glycerol as a plasticizer on the film performances was evaluated.
26572340	5	48	theme	mechanical	952:961	arg1	performance					982:992	better thermal, mechanical and oxygen barrier performance	936:992	better thermal, mechanical and oxygen barrier performance	936:992	In general, an increase in microfibrillated cellulose resulted in a film with better thermal, mechanical and oxygen barrier performance.
26572340	3	49	theme	permeability	678:689	arg1	analyses					691:698	thermal, structural, mechanical and oxygen permeability analyses	635:698	thermal, structural, mechanical and oxygen permeability analyses	635:698	Their capability as polymeric matrices was assessed based on thermal, structural, mechanical and oxygen permeability analyses.
26572340	6	50	theme	films	1109:1113	arg1	stiffness					1062:1070	stiffness	1062:1070	stiffness	1062:1070	However, the presence of glycerol decreased the thermal stability, stiffness and oxygen barrier properties of the films but improved their elongation.
26572340	6	50	theme	films	1109:1113	arg1	stability					1051:1059	the thermal stability	1039:1059	the thermal stability	1039:1059	However, the presence of glycerol decreased the thermal stability, stiffness and oxygen barrier properties of the films but improved their elongation.
26572340	6	50	theme	films	1109:1113	arg1	properties					1091:1100	oxygen barrier properties	1076:1100	oxygen barrier properties	1076:1100	However, the presence of glycerol decreased the thermal stability, stiffness and oxygen barrier properties of the films but improved their elongation.
26572340	2	51	theme	first	407:411	arg1	this					395:398	this	395:398	this	395:398	To the best of our knowledge, this is the first time that cross-linked galactoglucomannan-lignin networks have been used for the potential development of composite films inspired by woody-cell wall formation.
26572340	2	51	theme	first	407:411	arg1	time					413:416	the first time	403:416	the first time that cross-linked galactoglucomannan-lignin networks have been used for the potential development of composite films inspired by woody-cell wall formation	403:571	To the best of our knowledge, this is the first time that cross-linked galactoglucomannan-lignin networks have been used for the potential development of composite films inspired by woody-cell wall formation.
26572340	0	52	dep	composites	12:21	arg1	Thermal					92:98	Thermal	92:98	Thermal	92:98	Bioinspired composites from cross-linked galactoglucomannan and microfibrillated cellulose: Thermal, mechanical and oxygen barrier properties.
26572340	0	52	dep	composites	12:21	arg1	properties					131:140	oxygen barrier properties	116:140	oxygen barrier properties	116:140	Bioinspired composites from cross-linked galactoglucomannan and microfibrillated cellulose: Thermal, mechanical and oxygen barrier properties.
26572340	4	53	theme	reinforcing	770:780	arg1	agent					782:786	a reinforcing agent	768:786	a reinforcing agent	768:786	The addition of different amounts of microfibrillated cellulose as a reinforcing agent and glycerol as a plasticizer on the film performances was evaluated.
26572340	5	54	theme	thermal	943:949	arg1	performance					982:992	better thermal, mechanical and oxygen barrier performance	936:992	better thermal, mechanical and oxygen barrier performance	936:992	In general, an increase in microfibrillated cellulose resulted in a film with better thermal, mechanical and oxygen barrier performance.
26572340	1	55	theme	chemothermomechanical	282:302	arg1	streams					317:323	chemothermomechanical pulping side streams	282:323	chemothermomechanical pulping side streams	282:323	In this study, new wood-inspired films were developed from microfibrillated cellulose and galactoglucomannan-lignin networks isolated from chemothermomechanical pulping side streams and cross-linked using laccase enzymes.
26572340	7	56	from	agent	1263:1267	arg1	formulation					1297:1307	the film formulation	1288:1307	the film formulation	1288:1307	Therefore, depending on the application, the film properties can be tailored by adjusting the amounts of reinforcing agent and plasticizer in the film formulation.
26572340	1	57	theme	new	158:160	arg1	films					176:180	new wood-inspired films	158:180	new wood-inspired films	158:180	In this study, new wood-inspired films were developed from microfibrillated cellulose and galactoglucomannan-lignin networks isolated from chemothermomechanical pulping side streams and cross-linked using laccase enzymes.
26572340	1	58	theme	pulping	304:310	arg1	streams					317:323	chemothermomechanical pulping side streams	282:323	chemothermomechanical pulping side streams	282:323	In this study, new wood-inspired films were developed from microfibrillated cellulose and galactoglucomannan-lignin networks isolated from chemothermomechanical pulping side streams and cross-linked using laccase enzymes.
28629160	0	0	theme	Inhibitory	82:91	arg1	Effect					93:98	Its Inhibitory Effect	78:98	Its Inhibitory Effect on Galectin-3	78:112	Structural Characterization of a Rhamnogalacturonan I Domain from Ginseng and Its Inhibitory Effect on Galectin-3.
28629160	3	1	theme	detection	499:507	arg1	analysis					526:533	Partial acid hydrolysis, monoclonal antibody detection, and NMR spectra analysis	454:533	Partial acid hydrolysis, monoclonal antibody detection, and NMR spectra analysis	454:533	Partial acid hydrolysis, monoclonal antibody detection, and NMR spectra analysis suggested RG-I-3A was composed of →4)-α-GalpA-(1→2)-α-Rhap-(1→disaccharide repeating units as backbone, with β-1,4-galactan, α-1,5-arabinan, AG-I, and AG-II side chains substituted via the O-4 of Rhap.
28629160	3	2	theme	side	692:695	arg1	chains					697:702	β-1,4-galactan, α-1,5-arabinan, AG-I, and AG-II side chains	644:702	β-1,4-galactan, α-1,5-arabinan, AG-I, and AG-II side chains substituted via the O-4 of Rhap	644:734	Partial acid hydrolysis, monoclonal antibody detection, and NMR spectra analysis suggested RG-I-3A was composed of →4)-α-GalpA-(1→2)-α-Rhap-(1→disaccharide repeating units as backbone, with β-1,4-galactan, α-1,5-arabinan, AG-I, and AG-II side chains substituted via the O-4 of Rhap.
28629160	0	3	from	Characterization	11:26	arg1	Ginseng					66:72	Ginseng	66:72	Ginseng	66:72	Structural Characterization of a Rhamnogalacturonan I Domain from Ginseng and Its Inhibitory Effect on Galectin-3.
28629160	0	3	from	Characterization	11:26	arg1	Galectin-3					103:112	Galectin-3	103:112	Galectin-3	103:112	Structural Characterization of a Rhamnogalacturonan I Domain from Ginseng and Its Inhibitory Effect on Galectin-3.
28629160	2	4	from	rhamnose	343:350	arg1	ratio					389:393	a molar ratio	381:393	a molar ratio of 32.5:11.2:31.9:16.5	381:416	Monosaccharide composition analysis revealed that it was mainly composed of galacturonic acid, rhamnose, galactose, and arabinose in a molar ratio of 32.5:11.2:31.9:16.5, with a molecular weight of 50 kDa.
28629160	5	5	theme	drug	968:971	arg1	applications					985:996	drug development applications	968:996	drug development applications	968:996	These findings suggest the potential use of this ginseng RG-I domain as a galectin-3 inhibitor in drug development applications.
28629160	2	6	from	acid	337:340	arg1	ratio					389:393	a molar ratio	381:393	a molar ratio of 32.5:11.2:31.9:16.5	381:416	Monosaccharide composition analysis revealed that it was mainly composed of galacturonic acid, rhamnose, galactose, and arabinose in a molar ratio of 32.5:11.2:31.9:16.5, with a molecular weight of 50 kDa.
28629160	3	7	theme	α-1,5-arabinan	660:673	arg1	chains					697:702	β-1,4-galactan, α-1,5-arabinan, AG-I, and AG-II side chains	644:702	β-1,4-galactan, α-1,5-arabinan, AG-I, and AG-II side chains substituted via the O-4 of Rhap	644:734	Partial acid hydrolysis, monoclonal antibody detection, and NMR spectra analysis suggested RG-I-3A was composed of →4)-α-GalpA-(1→2)-α-Rhap-(1→disaccharide repeating units as backbone, with β-1,4-galactan, α-1,5-arabinan, AG-I, and AG-II side chains substituted via the O-4 of Rhap.
28629160	2	8	from	galactose	353:361	arg1	ratio					389:393	a molar ratio	381:393	a molar ratio of 32.5:11.2:31.9:16.5	381:416	Monosaccharide composition analysis revealed that it was mainly composed of galacturonic acid, rhamnose, galactose, and arabinose in a molar ratio of 32.5:11.2:31.9:16.5, with a molecular weight of 50 kDa.
28629160	5	9	theme	potential	897:905	arg1	use					907:909	the potential use	893:909	the potential use of this ginseng RG-I domain as a galectin-3 inhibitor in drug development applications	893:996	These findings suggest the potential use of this ginseng RG-I domain as a galectin-3 inhibitor in drug development applications.
28629160	4	10	theme	inhibitory	835:844	arg1	activity					846:853	inhibitory activity	835:853	inhibitory activity	835:853	Galectin-3-mediated hemagglutination and biolayer interferometry assay indicated that RG-I-3A had inhibitory activity on galectin-3.
28629160	3	11	dep	-α-Rhap-	586:593	arg1	1→2					582:584	1→2	582:584	1→2	582:584	Partial acid hydrolysis, monoclonal antibody detection, and NMR spectra analysis suggested RG-I-3A was composed of →4)-α-GalpA-(1→2)-α-Rhap-(1→disaccharide repeating units as backbone, with β-1,4-galactan, α-1,5-arabinan, AG-I, and AG-II side chains substituted via the O-4 of Rhap.
28629160	3	11	dep	-α-Rhap-	586:593	arg1	→4					569:570	→4	569:570	→4	569:570	Partial acid hydrolysis, monoclonal antibody detection, and NMR spectra analysis suggested RG-I-3A was composed of →4)-α-GalpA-(1→2)-α-Rhap-(1→disaccharide repeating units as backbone, with β-1,4-galactan, α-1,5-arabinan, AG-I, and AG-II side chains substituted via the O-4 of Rhap.
28629160	3	12	theme	spectra	518:524	arg1	analysis					526:533	Partial acid hydrolysis, monoclonal antibody detection, and NMR spectra analysis	454:533	Partial acid hydrolysis, monoclonal antibody detection, and NMR spectra analysis	454:533	Partial acid hydrolysis, monoclonal antibody detection, and NMR spectra analysis suggested RG-I-3A was composed of →4)-α-GalpA-(1→2)-α-Rhap-(1→disaccharide repeating units as backbone, with β-1,4-galactan, α-1,5-arabinan, AG-I, and AG-II side chains substituted via the O-4 of Rhap.
28629160	5	13	theme	ginseng	919:925	arg1	domain					932:937	this ginseng RG-I domain	914:937	this ginseng RG-I domain	914:937	These findings suggest the potential use of this ginseng RG-I domain as a galectin-3 inhibitor in drug development applications.
28629160	2	14	theme	kDa	449:451	arg1	weight					436:441	a molecular weight	424:441	a molecular weight of 50 kDa	424:451	Monosaccharide composition analysis revealed that it was mainly composed of galacturonic acid, rhamnose, galactose, and arabinose in a molar ratio of 32.5:11.2:31.9:16.5, with a molecular weight of 50 kDa.
28629160	5	15	theme	development	973:983	arg1	applications					985:996	drug development applications	968:996	drug development applications	968:996	These findings suggest the potential use of this ginseng RG-I domain as a galectin-3 inhibitor in drug development applications.
28629160	3	16	theme	AG-II	686:690	arg1	chains					697:702	β-1,4-galactan, α-1,5-arabinan, AG-I, and AG-II side chains	644:702	β-1,4-galactan, α-1,5-arabinan, AG-I, and AG-II side chains substituted via the O-4 of Rhap	644:734	Partial acid hydrolysis, monoclonal antibody detection, and NMR spectra analysis suggested RG-I-3A was composed of →4)-α-GalpA-(1→2)-α-Rhap-(1→disaccharide repeating units as backbone, with β-1,4-galactan, α-1,5-arabinan, AG-I, and AG-II side chains substituted via the O-4 of Rhap.
28629160	5	17	theme	domain	932:937	arg1	use					907:909	the potential use	893:909	the potential use of this ginseng RG-I domain as a galectin-3 inhibitor in drug development applications	893:996	These findings suggest the potential use of this ginseng RG-I domain as a galectin-3 inhibitor in drug development applications.
28629160	2	18	theme	molar	383:387	arg1	ratio					389:393	a molar ratio	381:393	a molar ratio of 32.5:11.2:31.9:16.5	381:416	Monosaccharide composition analysis revealed that it was mainly composed of galacturonic acid, rhamnose, galactose, and arabinose in a molar ratio of 32.5:11.2:31.9:16.5, with a molecular weight of 50 kDa.
28629160	0	19	theme	Structural	0:9	arg1	Characterization					11:26	Structural Characterization	0:26	Structural Characterization of a Rhamnogalacturonan I Domain from Ginseng	0:72	Structural Characterization of a Rhamnogalacturonan I Domain from Ginseng and Its Inhibitory Effect on Galectin-3.
28629160	2	20	theme	galacturonic	324:335	arg1	acid					337:340	galacturonic acid	324:340	galacturonic acid	324:340	Monosaccharide composition analysis revealed that it was mainly composed of galacturonic acid, rhamnose, galactose, and arabinose in a molar ratio of 32.5:11.2:31.9:16.5, with a molecular weight of 50 kDa.
28629160	1	21	theme	ginseng	179:185	arg1	pectin					187:192	ginseng pectin	179:192	ginseng pectin	179:192	A rhamnogalacturonan I domain, named RG-I-3A, was prepared from ginseng pectin by pectinase digestion and chromatography separation.
28629160	4	22	theme	interferometry	787:800	arg1	assay					802:806	Galectin-3-mediated hemagglutination and biolayer interferometry assay	737:806	assay	802:806	Galectin-3-mediated hemagglutination and biolayer interferometry assay indicated that RG-I-3A had inhibitory activity on galectin-3.
28629160	2	23	from	arabinose	368:376	arg1	ratio					389:393	a molar ratio	381:393	a molar ratio of 32.5:11.2:31.9:16.5	381:416	Monosaccharide composition analysis revealed that it was mainly composed of galacturonic acid, rhamnose, galactose, and arabinose in a molar ratio of 32.5:11.2:31.9:16.5, with a molecular weight of 50 kDa.
28629160	3	24	theme	Partial	454:460	arg1	hydrolysis					467:476	Partial acid hydrolysis	454:476	Partial acid hydrolysis	454:476	Partial acid hydrolysis, monoclonal antibody detection, and NMR spectra analysis suggested RG-I-3A was composed of →4)-α-GalpA-(1→2)-α-Rhap-(1→disaccharide repeating units as backbone, with β-1,4-galactan, α-1,5-arabinan, AG-I, and AG-II side chains substituted via the O-4 of Rhap.
28629160	2	25	theme	molecular	426:434	arg1	weight					436:441	a molecular weight	424:441	a molecular weight of 50 kDa	424:451	Monosaccharide composition analysis revealed that it was mainly composed of galacturonic acid, rhamnose, galactose, and arabinose in a molar ratio of 32.5:11.2:31.9:16.5, with a molecular weight of 50 kDa.
28629160	3	26	dep	suggested	535:543	arg1	composed					557:564	composed	557:564	was composed of →4)-α-GalpA-(1→2)-α-Rhap-	553:593	Partial acid hydrolysis, monoclonal antibody detection, and NMR spectra analysis suggested RG-I-3A was composed of →4)-α-GalpA-(1→2)-α-Rhap-(1→disaccharide repeating units as backbone, with β-1,4-galactan, α-1,5-arabinan, AG-I, and AG-II side chains substituted via the O-4 of Rhap.
28629160	5	27	theme	galectin-3	944:953	arg1	inhibitor					955:963	a galectin-3 inhibitor	942:963	a galectin-3 inhibitor in drug development applications	942:996	These findings suggest the potential use of this ginseng RG-I domain as a galectin-3 inhibitor in drug development applications.
28629160	3	28	theme	acid	462:465	arg1	hydrolysis					467:476	Partial acid hydrolysis	454:476	Partial acid hydrolysis	454:476	Partial acid hydrolysis, monoclonal antibody detection, and NMR spectra analysis suggested RG-I-3A was composed of →4)-α-GalpA-(1→2)-α-Rhap-(1→disaccharide repeating units as backbone, with β-1,4-galactan, α-1,5-arabinan, AG-I, and AG-II side chains substituted via the O-4 of Rhap.
28629160	0	29	theme	I	52:52	arg1	Domain					54:59	a Rhamnogalacturonan I Domain	31:59	a Rhamnogalacturonan I Domain from Ginseng	31:72	Structural Characterization of a Rhamnogalacturonan I Domain from Ginseng and Its Inhibitory Effect on Galectin-3.
28629160	3	30	theme	antibody	490:497	arg1	detection					499:507	monoclonal antibody detection	479:507	monoclonal antibody detection	479:507	Partial acid hydrolysis, monoclonal antibody detection, and NMR spectra analysis suggested RG-I-3A was composed of →4)-α-GalpA-(1→2)-α-Rhap-(1→disaccharide repeating units as backbone, with β-1,4-galactan, α-1,5-arabinan, AG-I, and AG-II side chains substituted via the O-4 of Rhap.
28629160	5	31	from	inhibitor	955:963	arg1	applications					985:996	drug development applications	968:996	drug development applications	968:996	These findings suggest the potential use of this ginseng RG-I domain as a galectin-3 inhibitor in drug development applications.
28629160	4	32	theme	biolayer	778:785	arg1	assay					802:806	Galectin-3-mediated hemagglutination and biolayer interferometry assay	737:806	assay	802:806	Galectin-3-mediated hemagglutination and biolayer interferometry assay indicated that RG-I-3A had inhibitory activity on galectin-3.
28629160	1	33	theme	pectinase	197:205	arg1	digestion					207:215	pectinase digestion	197:215	pectinase digestion	197:215	A rhamnogalacturonan I domain, named RG-I-3A, was prepared from ginseng pectin by pectinase digestion and chromatography separation.
28629160	0	34	theme	Rhamnogalacturonan	33:50	arg1	Domain					54:59	a Rhamnogalacturonan I Domain	31:59	a Rhamnogalacturonan I Domain from Ginseng	31:72	Structural Characterization of a Rhamnogalacturonan I Domain from Ginseng and Its Inhibitory Effect on Galectin-3.
28629160	3	35	theme	repeating	610:618	arg1	units					620:624	1→disaccharide repeating units	595:624	1→disaccharide repeating units	595:624	Partial acid hydrolysis, monoclonal antibody detection, and NMR spectra analysis suggested RG-I-3A was composed of →4)-α-GalpA-(1→2)-α-Rhap-(1→disaccharide repeating units as backbone, with β-1,4-galactan, α-1,5-arabinan, AG-I, and AG-II side chains substituted via the O-4 of Rhap.
28629160	2	36	theme	32.5:11.2:31.9:16.5	398:416	arg1	ratio					389:393	a molar ratio	381:393	a molar ratio of 32.5:11.2:31.9:16.5	381:416	Monosaccharide composition analysis revealed that it was mainly composed of galacturonic acid, rhamnose, galactose, and arabinose in a molar ratio of 32.5:11.2:31.9:16.5, with a molecular weight of 50 kDa.
28629160	0	37	from	Ginseng	66:72	arg1	Domain					54:59	a Rhamnogalacturonan I Domain	31:59	a Rhamnogalacturonan I Domain from Ginseng	31:72	Structural Characterization of a Rhamnogalacturonan I Domain from Ginseng and Its Inhibitory Effect on Galectin-3.
28629160	0	37	from	Ginseng	66:72	arg1	Characterization					11:26	Structural Characterization	0:26	Structural Characterization of a Rhamnogalacturonan I Domain from Ginseng	0:72	Structural Characterization of a Rhamnogalacturonan I Domain from Ginseng and Its Inhibitory Effect on Galectin-3.
28629160	0	37	from	Ginseng	66:72	arg1	Effect					93:98	Its Inhibitory Effect	78:98	Its Inhibitory Effect on Galectin-3	78:112	Structural Characterization of a Rhamnogalacturonan I Domain from Ginseng and Its Inhibitory Effect on Galectin-3.
28629160	3	38	theme	monoclonal	479:488	arg1	antibody					490:497	monoclonal antibody	479:497	monoclonal antibody detection	479:507	Partial acid hydrolysis, monoclonal antibody detection, and NMR spectra analysis suggested RG-I-3A was composed of →4)-α-GalpA-(1→2)-α-Rhap-(1→disaccharide repeating units as backbone, with β-1,4-galactan, α-1,5-arabinan, AG-I, and AG-II side chains substituted via the O-4 of Rhap.
28629160	0	39	theme	Domain	54:59	arg1	Characterization					11:26	Structural Characterization	0:26	Structural Characterization of a Rhamnogalacturonan I Domain from Ginseng	0:72	Structural Characterization of a Rhamnogalacturonan I Domain from Ginseng and Its Inhibitory Effect on Galectin-3.
28629160	0	39	theme	Domain	54:59	arg1	Effect					93:98	Its Inhibitory Effect	78:98	Its Inhibitory Effect on Galectin-3	78:112	Structural Characterization of a Rhamnogalacturonan I Domain from Ginseng and Its Inhibitory Effect on Galectin-3.
28629160	3	40	theme	hydrolysis	467:476	arg1	analysis					526:533	Partial acid hydrolysis, monoclonal antibody detection, and NMR spectra analysis	454:533	Partial acid hydrolysis, monoclonal antibody detection, and NMR spectra analysis	454:533	Partial acid hydrolysis, monoclonal antibody detection, and NMR spectra analysis suggested RG-I-3A was composed of →4)-α-GalpA-(1→2)-α-Rhap-(1→disaccharide repeating units as backbone, with β-1,4-galactan, α-1,5-arabinan, AG-I, and AG-II side chains substituted via the O-4 of Rhap.
28629160	3	41	theme	β-1,4-galactan	644:657	arg1	chains					697:702	β-1,4-galactan, α-1,5-arabinan, AG-I, and AG-II side chains	644:702	β-1,4-galactan, α-1,5-arabinan, AG-I, and AG-II side chains substituted via the O-4 of Rhap	644:734	Partial acid hydrolysis, monoclonal antibody detection, and NMR spectra analysis suggested RG-I-3A was composed of →4)-α-GalpA-(1→2)-α-Rhap-(1→disaccharide repeating units as backbone, with β-1,4-galactan, α-1,5-arabinan, AG-I, and AG-II side chains substituted via the O-4 of Rhap.
28629160	3	42	theme	-α-GalpA-	572:580	arg1	-α-Rhap-					586:593	→4)-α-GalpA-(1→2)-α-Rhap-	569:593	→4)-α-GalpA-(1→2)-α-Rhap-	569:593	Partial acid hydrolysis, monoclonal antibody detection, and NMR spectra analysis suggested RG-I-3A was composed of →4)-α-GalpA-(1→2)-α-Rhap-(1→disaccharide repeating units as backbone, with β-1,4-galactan, α-1,5-arabinan, AG-I, and AG-II side chains substituted via the O-4 of Rhap.
28629160	1	43	theme	rhamnogalacturonan	117:134	arg1	domain					138:143	A rhamnogalacturonan I domain	115:143	A rhamnogalacturonan I domain	115:143	A rhamnogalacturonan I domain, named RG-I-3A, was prepared from ginseng pectin by pectinase digestion and chromatography separation.
28629160	3	44	theme	Rhap	731:734	arg1	O-4					724:726	the O-4	720:726	the O-4 of Rhap	720:734	Partial acid hydrolysis, monoclonal antibody detection, and NMR spectra analysis suggested RG-I-3A was composed of →4)-α-GalpA-(1→2)-α-Rhap-(1→disaccharide repeating units as backbone, with β-1,4-galactan, α-1,5-arabinan, AG-I, and AG-II side chains substituted via the O-4 of Rhap.
28629160	1	45	theme	I	136:136	arg1	domain					138:143	A rhamnogalacturonan I domain	115:143	A rhamnogalacturonan I domain	115:143	A rhamnogalacturonan I domain, named RG-I-3A, was prepared from ginseng pectin by pectinase digestion and chromatography separation.
28629160	4	46	theme	Galectin-3-mediated	737:755	arg1	hemagglutination					757:772	Galectin-3-mediated hemagglutination and biolayer interferometry assay	737:806	hemagglutination	757:772	Galectin-3-mediated hemagglutination and biolayer interferometry assay indicated that RG-I-3A had inhibitory activity on galectin-3.
28629160	3	47	theme	1→disaccharide	595:608	arg1	units					620:624	1→disaccharide repeating units	595:624	1→disaccharide repeating units	595:624	Partial acid hydrolysis, monoclonal antibody detection, and NMR spectra analysis suggested RG-I-3A was composed of →4)-α-GalpA-(1→2)-α-Rhap-(1→disaccharide repeating units as backbone, with β-1,4-galactan, α-1,5-arabinan, AG-I, and AG-II side chains substituted via the O-4 of Rhap.
28629160	5	48	theme	RG-I	927:930	arg1	domain					932:937	this ginseng RG-I domain	914:937	this ginseng RG-I domain	914:937	These findings suggest the potential use of this ginseng RG-I domain as a galectin-3 inhibitor in drug development applications.
28629160	4	49	contain	had	831:833	arg2	activity					846:853	inhibitory activity	835:853	inhibitory activity	835:853	Galectin-3-mediated hemagglutination and biolayer interferometry assay indicated that RG-I-3A had inhibitory activity on galectin-3.
28629160	4	49	contain	had	831:833	arg1	RG-I-3A					823:829	RG-I-3A	823:829	RG-I-3A	823:829	Galectin-3-mediated hemagglutination and biolayer interferometry assay indicated that RG-I-3A had inhibitory activity on galectin-3.
28629160	1	50	theme	chromatography	221:234	arg1	separation					236:245	chromatography separation	221:245	chromatography separation	221:245	A rhamnogalacturonan I domain, named RG-I-3A, was prepared from ginseng pectin by pectinase digestion and chromatography separation.
28629160	0	51	from	Effect	93:98	arg1	Ginseng					66:72	Ginseng	66:72	Ginseng	66:72	Structural Characterization of a Rhamnogalacturonan I Domain from Ginseng and Its Inhibitory Effect on Galectin-3.
28629160	0	51	from	Effect	93:98	arg1	Galectin-3					103:112	Galectin-3	103:112	Galectin-3	103:112	Structural Characterization of a Rhamnogalacturonan I Domain from Ginseng and Its Inhibitory Effect on Galectin-3.
28629160	2	52	theme	composition	263:273	arg1	analysis					275:282	Monosaccharide composition analysis	248:282	Monosaccharide composition analysis	248:282	Monosaccharide composition analysis revealed that it was mainly composed of galacturonic acid, rhamnose, galactose, and arabinose in a molar ratio of 32.5:11.2:31.9:16.5, with a molecular weight of 50 kDa.
28629160	3	53	theme	AG-I	676:679	arg1	chains					697:702	β-1,4-galactan, α-1,5-arabinan, AG-I, and AG-II side chains	644:702	β-1,4-galactan, α-1,5-arabinan, AG-I, and AG-II side chains substituted via the O-4 of Rhap	644:734	Partial acid hydrolysis, monoclonal antibody detection, and NMR spectra analysis suggested RG-I-3A was composed of →4)-α-GalpA-(1→2)-α-Rhap-(1→disaccharide repeating units as backbone, with β-1,4-galactan, α-1,5-arabinan, AG-I, and AG-II side chains substituted via the O-4 of Rhap.
28629160	2	54	theme	Monosaccharide	248:261	arg1	analysis					275:282	Monosaccharide composition analysis	248:282	Monosaccharide composition analysis	248:282	Monosaccharide composition analysis revealed that it was mainly composed of galacturonic acid, rhamnose, galactose, and arabinose in a molar ratio of 32.5:11.2:31.9:16.5, with a molecular weight of 50 kDa.
28629160	3	55	theme	NMR	514:516	arg1	spectra					518:524	NMR spectra	514:524	NMR spectra	514:524	Partial acid hydrolysis, monoclonal antibody detection, and NMR spectra analysis suggested RG-I-3A was composed of →4)-α-GalpA-(1→2)-α-Rhap-(1→disaccharide repeating units as backbone, with β-1,4-galactan, α-1,5-arabinan, AG-I, and AG-II side chains substituted via the O-4 of Rhap.
26988509	0	0	theme	nutraceutical	76:88	arg1	applications					90:101	food and nutraceutical applications	67:101	food and nutraceutical applications	67:101	Microencapsulation of Nigella sativa oleoresin by spray drying for food and nutraceutical applications.
26988509	4	1	theme	chromatographic-mass	664:683	arg1	analysis					699:706	gas chromatographic-mass spectroscopic analysis	660:706	gas chromatographic-mass spectroscopic analysis	660:706	The effect of the spray drying on the chemical composition of the volatile oil fraction of N. sativa oleoresin was also evaluated using gas chromatographic-mass spectroscopic analysis.
26988509	6	2	theme	oil	974:976	arg1	fraction					978:985	the volatile oil fraction	961:985	the volatile oil fraction	961:985	On the other hand the encapsulation efficiency of the volatile oil fraction was 86.2% ±4.7.
26988509	5	3	theme	whole	768:772	arg1	oleoresin					774:782	the whole oleoresin	764:782	the whole oleoresin	764:782	Results indicated that the encapsulation efficiency of the whole oleoresin in the powder can range from 84.2±1.5% to 96.2±0.2% depending on the conditions of extracting the surface oil from the powder.
26988509	4	4	theme	gas	660:662	arg1	analysis					699:706	gas chromatographic-mass spectroscopic analysis	660:706	gas chromatographic-mass spectroscopic analysis	660:706	The effect of the spray drying on the chemical composition of the volatile oil fraction of N. sativa oleoresin was also evaluated using gas chromatographic-mass spectroscopic analysis.
26988509	4	5	theme	drying	548:553	arg1	effect					528:533	The effect	524:533	The effect of the spray drying on the chemical composition of the volatile oil fraction of N. sativa oleoresin	524:633	The effect of the spray drying on the chemical composition of the volatile oil fraction of N. sativa oleoresin was also evaluated using gas chromatographic-mass spectroscopic analysis.
26988509	6	6	from	%	995:995	arg1	hand					924:927	the other hand	914:927	the other hand	914:927	On the other hand the encapsulation efficiency of the volatile oil fraction was 86.2% ±4.7.
26988509	4	7	theme	oleoresin	625:633	arg1	fraction					603:610	the volatile oil fraction	586:610	the volatile oil fraction of N. sativa oleoresin	586:633	The effect of the spray drying on the chemical composition of the volatile oil fraction of N. sativa oleoresin was also evaluated using gas chromatographic-mass spectroscopic analysis.
26988509	4	8	theme	chemical	562:569	arg1	composition					571:581	the chemical composition	558:581	the chemical composition of the volatile oil fraction of N. sativa oleoresin	558:633	The effect of the spray drying on the chemical composition of the volatile oil fraction of N. sativa oleoresin was also evaluated using gas chromatographic-mass spectroscopic analysis.
26988509	2	9	theme	aqueous	257:263	arg1	solution					265:272	an aqueous solution	254:272	an aqueous solution containing gum Arabic/maltodextrin (1:1 w/w)	254:317	The oleoresin was emulsified in an aqueous solution containing gum Arabic/maltodextrin (1:1 w/w) and then encapsulated in powder form by spray drying.
26988509	5	10	theme	96.2±0.2	826:833	arg1	%					821:821	%	821:821	%	821:821	Results indicated that the encapsulation efficiency of the whole oleoresin in the powder can range from 84.2±1.5% to 96.2±0.2% depending on the conditions of extracting the surface oil from the powder.
26988509	7	11	used	used	1055:1058	arg2	powder					1041:1046	The formulated N. sativa L. oleoresin powder	1003:1046	The formulated N. sativa L. oleoresin powder	1003:1046	The formulated N. sativa L. oleoresin powder can be used in the fortification of processed food and nutraceuticals.
26988509	4	12	theme	oil	599:601	arg1	fraction					603:610	the volatile oil fraction	586:610	the volatile oil fraction of N. sativa oleoresin	586:633	The effect of the spray drying on the chemical composition of the volatile oil fraction of N. sativa oleoresin was also evaluated using gas chromatographic-mass spectroscopic analysis.
26988509	1	13	dep	Nigella	117:123	arg1	L					132:132	L.	132:133	Nigella sativa L. (Black cumin)	117:147	Oleoresin of Nigella sativa L. (Black cumin) was obtained from the seeds using hexane extraction at room temperature.
26988509	1	13	dep	Nigella	117:123	arg1	cumin					142:146	Black cumin	136:146	Black cumin	136:146	Oleoresin of Nigella sativa L. (Black cumin) was obtained from the seeds using hexane extraction at room temperature.
26988509	1	13	dep	Nigella	117:123	arg1	sativa					125:130	Nigella sativa L. (Black cumin)	117:147	Nigella sativa L. (Black cumin)	117:147	Oleoresin of Nigella sativa L. (Black cumin) was obtained from the seeds using hexane extraction at room temperature.
26988509	3	14	theme	powder	409:414	arg1	content					435:441	moisture content	426:441	moisture content	426:441	The characteristics of the obtained powder including moisture content, bulk density, wettability, morphology, encapsulation efficiency were evaluated.
26988509	3	14	theme	powder	409:414	arg1	characteristics					377:391	The characteristics	373:391	The characteristics of the obtained powder including moisture content, bulk density, wettability, morphology, encapsulation efficiency	373:506	The characteristics of the obtained powder including moisture content, bulk density, wettability, morphology, encapsulation efficiency were evaluated.
26988509	5	15	theme	encapsulation	736:748	arg1	efficiency					750:759	the encapsulation efficiency	732:759	the encapsulation efficiency of the whole oleoresin in the powder	732:796	Results indicated that the encapsulation efficiency of the whole oleoresin in the powder can range from 84.2±1.5% to 96.2±0.2% depending on the conditions of extracting the surface oil from the powder.
26988509	7	16	theme	oleoresin	1031:1039	arg1	powder					1041:1046	The formulated N. sativa L. oleoresin powder	1003:1046	The formulated N. sativa L. oleoresin powder	1003:1046	The formulated N. sativa L. oleoresin powder can be used in the fortification of processed food and nutraceuticals.
26988509	2	17	theme	spray	359:363	arg1	drying					365:370	spray drying	359:370	spray drying	359:370	The oleoresin was emulsified in an aqueous solution containing gum Arabic/maltodextrin (1:1 w/w) and then encapsulated in powder form by spray drying.
26988509	7	18	dep	N.	1018:1019	arg1	L.					1028:1029	N. sativa L.	1018:1029	The formulated N. sativa L. oleoresin powder	1003:1046	The formulated N. sativa L. oleoresin powder can be used in the fortification of processed food and nutraceuticals.
26988509	4	19	theme	N.	615:616	arg1	oleoresin					625:633	N. sativa oleoresin	615:633	N. sativa oleoresin	615:633	The effect of the spray drying on the chemical composition of the volatile oil fraction of N. sativa oleoresin was also evaluated using gas chromatographic-mass spectroscopic analysis.
26988509	7	20	theme	nutraceuticals	1103:1116	arg1	fortification					1067:1079	the fortification	1063:1079	the fortification of processed food and nutraceuticals	1063:1116	The formulated N. sativa L. oleoresin powder can be used in the fortification of processed food and nutraceuticals.
26988509	3	21	theme	obtained	400:407	arg1	powder					409:414	the obtained powder	396:414	the obtained powder	396:414	The characteristics of the obtained powder including moisture content, bulk density, wettability, morphology, encapsulation efficiency were evaluated.
26988509	0	22	theme	sativa	30:35	arg1	oleoresin					37:45	Nigella sativa oleoresin	22:45	Nigella sativa oleoresin	22:45	Microencapsulation of Nigella sativa oleoresin by spray drying for food and nutraceutical applications.
26988509	6	23	theme	encapsulation	933:945	arg1	%					995:995	86.2% ±4.7	991:1000	86.2% ±4.7	991:1000	On the other hand the encapsulation efficiency of the volatile oil fraction was 86.2% ±4.7.
26988509	6	23	theme	encapsulation	933:945	arg1	efficiency					947:956	the encapsulation efficiency	929:956	the encapsulation efficiency of the volatile oil fraction	929:985	On the other hand the encapsulation efficiency of the volatile oil fraction was 86.2% ±4.7.
26988509	2	24	theme	gum	285:287	arg1	Arabic/maltodextrin					289:307	gum Arabic/maltodextrin	285:307	gum Arabic/maltodextrin (1:1 w/w)	285:317	The oleoresin was emulsified in an aqueous solution containing gum Arabic/maltodextrin (1:1 w/w) and then encapsulated in powder form by spray drying.
26988509	2	24	theme	gum	285:287	arg1	w/w					314:316	1:1 w/w	310:316	1:1 w/w	310:316	The oleoresin was emulsified in an aqueous solution containing gum Arabic/maltodextrin (1:1 w/w) and then encapsulated in powder form by spray drying.
26988509	4	25	theme	fraction	603:610	arg1	composition					571:581	the chemical composition	558:581	the chemical composition of the volatile oil fraction of N. sativa oleoresin	558:633	The effect of the spray drying on the chemical composition of the volatile oil fraction of N. sativa oleoresin was also evaluated using gas chromatographic-mass spectroscopic analysis.
26988509	0	26	theme	Nigella	22:28	arg1	oleoresin					37:45	Nigella sativa oleoresin	22:45	Nigella sativa oleoresin	22:45	Microencapsulation of Nigella sativa oleoresin by spray drying for food and nutraceutical applications.
26988509	4	27	theme	spray	542:546	arg1	drying					548:553	the spray drying	538:553	the spray drying	538:553	The effect of the spray drying on the chemical composition of the volatile oil fraction of N. sativa oleoresin was also evaluated using gas chromatographic-mass spectroscopic analysis.
26988509	2	28	theme	powder	344:349	arg1	form					351:354	powder form	344:354	powder form	344:354	The oleoresin was emulsified in an aqueous solution containing gum Arabic/maltodextrin (1:1 w/w) and then encapsulated in powder form by spray drying.
26988509	5	29	theme	surface	882:888	arg1	oil					890:892	the surface oil	878:892	the surface oil	878:892	Results indicated that the encapsulation efficiency of the whole oleoresin in the powder can range from 84.2±1.5% to 96.2±0.2% depending on the conditions of extracting the surface oil from the powder.
26988509	3	30	theme	bulk	444:447	arg1	content					435:441	moisture content	426:441	moisture content	426:441	The characteristics of the obtained powder including moisture content, bulk density, wettability, morphology, encapsulation efficiency were evaluated.
26988509	3	30	theme	bulk	444:447	arg1	density					449:455	bulk density	444:455	bulk density	444:455	The characteristics of the obtained powder including moisture content, bulk density, wettability, morphology, encapsulation efficiency were evaluated.
26988509	5	31	theme	oleoresin	774:782	arg1	efficiency					750:759	the encapsulation efficiency	732:759	the encapsulation efficiency of the whole oleoresin in the powder	732:796	Results indicated that the encapsulation efficiency of the whole oleoresin in the powder can range from 84.2±1.5% to 96.2±0.2% depending on the conditions of extracting the surface oil from the powder.
26988509	7	32	theme	N.	1018:1019	arg1	powder					1041:1046	The formulated N. sativa L. oleoresin powder	1003:1046	The formulated N. sativa L. oleoresin powder	1003:1046	The formulated N. sativa L. oleoresin powder can be used in the fortification of processed food and nutraceuticals.
26988509	3	33	theme	moisture	426:433	arg1	wettability					458:468	wettability	458:468	wettability	458:468	The characteristics of the obtained powder including moisture content, bulk density, wettability, morphology, encapsulation efficiency were evaluated.
26988509	3	33	theme	moisture	426:433	arg1	content					435:441	moisture content	426:441	moisture content	426:441	The characteristics of the obtained powder including moisture content, bulk density, wettability, morphology, encapsulation efficiency were evaluated.
26988509	3	33	theme	moisture	426:433	arg1	density					449:455	bulk density	444:455	bulk density	444:455	The characteristics of the obtained powder including moisture content, bulk density, wettability, morphology, encapsulation efficiency were evaluated.
26988509	3	33	theme	moisture	426:433	arg1	morphology					471:480	morphology	471:480	morphology	471:480	The characteristics of the obtained powder including moisture content, bulk density, wettability, morphology, encapsulation efficiency were evaluated.
26988509	3	33	theme	moisture	426:433	arg1	efficiency					497:506	encapsulation efficiency	483:506	encapsulation efficiency	483:506	The characteristics of the obtained powder including moisture content, bulk density, wettability, morphology, encapsulation efficiency were evaluated.
26988509	1	34	theme	hexane	183:188	arg1	extraction					190:199	hexane extraction	183:199	hexane extraction	183:199	Oleoresin of Nigella sativa L. (Black cumin) was obtained from the seeds using hexane extraction at room temperature.
26988509	0	35	theme	oleoresin	37:45	arg1	Microencapsulation					0:17	Microencapsulation	0:17	Microencapsulation of Nigella sativa oleoresin by spray drying for food and nutraceutical applications.	0:102	Microencapsulation of Nigella sativa oleoresin by spray drying for food and nutraceutical applications.
26988509	2	36	contain	containing	274:283	arg1	solution					265:272	an aqueous solution	254:272	an aqueous solution containing gum Arabic/maltodextrin (1:1 w/w)	254:317	The oleoresin was emulsified in an aqueous solution containing gum Arabic/maltodextrin (1:1 w/w) and then encapsulated in powder form by spray drying.
26988509	2	36	contain	containing	274:283	arg2	Arabic/maltodextrin					289:307	gum Arabic/maltodextrin	285:307	gum Arabic/maltodextrin (1:1 w/w)	285:317	The oleoresin was emulsified in an aqueous solution containing gum Arabic/maltodextrin (1:1 w/w) and then encapsulated in powder form by spray drying.
26988509	2	36	contain	containing	274:283	arg2	w/w					314:316	1:1 w/w	310:316	1:1 w/w	310:316	The oleoresin was emulsified in an aqueous solution containing gum Arabic/maltodextrin (1:1 w/w) and then encapsulated in powder form by spray drying.
26988509	6	37	theme	volatile	965:972	arg1	fraction					978:985	the volatile oil fraction	961:985	the volatile oil fraction	961:985	On the other hand the encapsulation efficiency of the volatile oil fraction was 86.2% ±4.7.
26988509	7	38	theme	processed	1084:1092	arg1	food					1094:1097	food	1094:1097	food	1094:1097	The formulated N. sativa L. oleoresin powder can be used in the fortification of processed food and nutraceuticals.
26988509	6	39	theme	other	918:922	arg1	hand					924:927	the other hand	914:927	the other hand	914:927	On the other hand the encapsulation efficiency of the volatile oil fraction was 86.2% ±4.7.
26988509	5	40	from	%	834:834	arg1	range					802:806	range	802:806	range	802:806	Results indicated that the encapsulation efficiency of the whole oleoresin in the powder can range from 84.2±1.5% to 96.2±0.2% depending on the conditions of extracting the surface oil from the powder.
26988509	1	41	theme	Nigella	117:123	arg1	Oleoresin					104:112	Oleoresin	104:112	Oleoresin of Nigella sativa L. (Black cumin)	104:147	Oleoresin of Nigella sativa L. (Black cumin) was obtained from the seeds using hexane extraction at room temperature.
26988509	0	42	theme	spray	50:54	arg1	drying					56:61	spray drying	50:61	spray drying for food and nutraceutical applications	50:101	Microencapsulation of Nigella sativa oleoresin by spray drying for food and nutraceutical applications.
26988509	7	43	theme	formulated	1007:1016	arg1	powder					1041:1046	The formulated N. sativa L. oleoresin powder	1003:1046	The formulated N. sativa L. oleoresin powder	1003:1046	The formulated N. sativa L. oleoresin powder can be used in the fortification of processed food and nutraceuticals.
26988509	4	44	theme	spectroscopic	685:697	arg1	analysis					699:706	gas chromatographic-mass spectroscopic analysis	660:706	gas chromatographic-mass spectroscopic analysis	660:706	The effect of the spray drying on the chemical composition of the volatile oil fraction of N. sativa oleoresin was also evaluated using gas chromatographic-mass spectroscopic analysis.
26988509	7	45	theme	food	1094:1097	arg1	fortification					1067:1079	the fortification	1063:1079	the fortification of processed food and nutraceuticals	1063:1116	The formulated N. sativa L. oleoresin powder can be used in the fortification of processed food and nutraceuticals.
26988509	5	46	from	efficiency	750:759	arg1	powder					791:796	the powder	787:796	the powder	787:796	Results indicated that the encapsulation efficiency of the whole oleoresin in the powder can range from 84.2±1.5% to 96.2±0.2% depending on the conditions of extracting the surface oil from the powder.
26988509	5	47	theme	84.2±1.5	813:820	arg1	%					821:821	%	821:821	%	821:821	Results indicated that the encapsulation efficiency of the whole oleoresin in the powder can range from 84.2±1.5% to 96.2±0.2% depending on the conditions of extracting the surface oil from the powder.
26988509	1	48	theme	room	204:207	arg1	temperature					209:219	room temperature	204:219	room temperature	204:219	Oleoresin of Nigella sativa L. (Black cumin) was obtained from the seeds using hexane extraction at room temperature.
26988509	0	49	theme	food	67:70	arg1	applications					90:101	food and nutraceutical applications	67:101	food and nutraceutical applications	67:101	Microencapsulation of Nigella sativa oleoresin by spray drying for food and nutraceutical applications.
26988509	5	50	dep	%	821:821	arg1	to					823:824	to	823:824	to	823:824	Results indicated that the encapsulation efficiency of the whole oleoresin in the powder can range from 84.2±1.5% to 96.2±0.2% depending on the conditions of extracting the surface oil from the powder.
26988509	3	51	theme	encapsulation	483:495	arg1	content					435:441	moisture content	426:441	moisture content	426:441	The characteristics of the obtained powder including moisture content, bulk density, wettability, morphology, encapsulation efficiency were evaluated.
26988509	3	51	theme	encapsulation	483:495	arg1	efficiency					497:506	encapsulation efficiency	483:506	encapsulation efficiency	483:506	The characteristics of the obtained powder including moisture content, bulk density, wettability, morphology, encapsulation efficiency were evaluated.
26988509	4	52	theme	sativa	618:623	arg1	oleoresin					625:633	N. sativa oleoresin	615:633	N. sativa oleoresin	615:633	The effect of the spray drying on the chemical composition of the volatile oil fraction of N. sativa oleoresin was also evaluated using gas chromatographic-mass spectroscopic analysis.
26988509	4	53	theme	volatile	590:597	arg1	fraction					603:610	the volatile oil fraction	586:610	the volatile oil fraction of N. sativa oleoresin	586:633	The effect of the spray drying on the chemical composition of the volatile oil fraction of N. sativa oleoresin was also evaluated using gas chromatographic-mass spectroscopic analysis.
26988509	4	54	from	effect	528:533	arg1	composition					571:581	the chemical composition	558:581	the chemical composition of the volatile oil fraction of N. sativa oleoresin	558:633	The effect of the spray drying on the chemical composition of the volatile oil fraction of N. sativa oleoresin was also evaluated using gas chromatographic-mass spectroscopic analysis.
26988509	1	55	theme	Black	136:140	arg1	L					132:132	L.	132:133	Nigella sativa L. (Black cumin)	117:147	Oleoresin of Nigella sativa L. (Black cumin) was obtained from the seeds using hexane extraction at room temperature.
26988509	1	55	theme	Black	136:140	arg1	cumin					142:146	Black cumin	136:146	Black cumin	136:146	Oleoresin of Nigella sativa L. (Black cumin) was obtained from the seeds using hexane extraction at room temperature.
26988509	6	56	theme	fraction	978:985	arg1	%					995:995	86.2% ±4.7	991:1000	86.2% ±4.7	991:1000	On the other hand the encapsulation efficiency of the volatile oil fraction was 86.2% ±4.7.
26988509	6	56	theme	fraction	978:985	arg1	efficiency					947:956	the encapsulation efficiency	929:956	the encapsulation efficiency of the volatile oil fraction	929:985	On the other hand the encapsulation efficiency of the volatile oil fraction was 86.2% ±4.7.
29233711	8	0	theme	anticancer	1426:1435	arg1	agent					1437:1441	a natural anticancer agent	1416:1441	a natural anticancer agent with lower cost and cytotoxicity	1416:1474	These findings for the first time reveal the potential anti-tumor mechanism of SMP, suggesting that SMP may serve a natural anticancer agent with lower cost and cytotoxicity, as well as a bioactive factor for functional food development.
29233711	4	1	theme	hydroxyl	826:833	arg1	radicals					840:847	hydroxyl free radicals	826:847	hydroxyl free radicals	826:847	In addition, the antioxidant potential of SMP was evaluated in terms of reducing power, scavenging ability against DPPH, superoxide and hydroxyl free radicals.
29233711	6	2	theme	tumor	1072:1076	arg1	cells					1078:1082	typical tumor cells	1064:1082	typical tumor cells	1064:1082	Furthermore, SMP is observed with high inhibition ratio against LoVo cells (typical tumor cells) in both dose- and time-dependent manners.
29233711	6	2	theme	tumor	1072:1076	arg1	cells					1057:1061	LoVo cells	1052:1061	LoVo cells (typical tumor cells)	1052:1083	Furthermore, SMP is observed with high inhibition ratio against LoVo cells (typical tumor cells) in both dose- and time-dependent manners.
29233711	5	3	theme	versatile	927:935	arg1	activities					949:958	versatile antioxidant activities	927:958	versatile antioxidant activities	927:958	The results indicate that polysaccharides from S. miltiorrhiza Bunge possess versatile antioxidant activities in a dose-dependent manner.
29233711	0	4	from	effect	10:15	arg1	Bunge					97:101	Bunge	97:101	Bunge	97:101	Antitumor effect and molecular mechanism of antioxidant polysaccharides from Salvia miltiorrhiza Bunge in human colorectal carcinoma LoVo cells.
29233711	0	4	from	effect	10:15	arg1	cells					138:142	human colorectal carcinoma LoVo cells	106:142	human colorectal carcinoma LoVo cells	106:142	Antitumor effect and molecular mechanism of antioxidant polysaccharides from Salvia miltiorrhiza Bunge in human colorectal carcinoma LoVo cells.
29233711	4	5	theme	free	835:838	arg1	radicals					840:847	hydroxyl free radicals	826:847	hydroxyl free radicals	826:847	In addition, the antioxidant potential of SMP was evaluated in terms of reducing power, scavenging ability against DPPH, superoxide and hydroxyl free radicals.
29233711	0	6	theme	human	106:110	arg1	cells					138:142	human colorectal carcinoma LoVo cells	106:142	human colorectal carcinoma LoVo cells	106:142	Antitumor effect and molecular mechanism of antioxidant polysaccharides from Salvia miltiorrhiza Bunge in human colorectal carcinoma LoVo cells.
29233711	8	7	theme	anti-tumor	1357:1366	arg1	mechanism					1368:1376	the potential anti-tumor mechanism	1343:1376	the potential anti-tumor mechanism of SMP	1343:1383	These findings for the first time reveal the potential anti-tumor mechanism of SMP, suggesting that SMP may serve a natural anticancer agent with lower cost and cytotoxicity, as well as a bioactive factor for functional food development.
29233711	8	8	theme	potential	1347:1355	arg1	mechanism					1368:1376	the potential anti-tumor mechanism	1343:1376	the potential anti-tumor mechanism of SMP	1343:1383	These findings for the first time reveal the potential anti-tumor mechanism of SMP, suggesting that SMP may serve a natural anticancer agent with lower cost and cytotoxicity, as well as a bioactive factor for functional food development.
29233711	4	9	theme	SMP	732:734	arg1	potential					719:727	the antioxidant potential	703:727	the antioxidant potential of SMP	703:734	In addition, the antioxidant potential of SMP was evaluated in terms of reducing power, scavenging ability against DPPH, superoxide and hydroxyl free radicals.
29233711	4	10	theme	antioxidant	707:717	arg1	potential					719:727	the antioxidant potential	703:727	the antioxidant potential of SMP	703:734	In addition, the antioxidant potential of SMP was evaluated in terms of reducing power, scavenging ability against DPPH, superoxide and hydroxyl free radicals.
29233711	3	11	theme	HPLC-UV	429:435	arg1	protocol					437:444	the established HPLC-UV protocol	413:444	the established HPLC-UV protocol with PMP precolumn derivatization	413:478	The monosaccharide composition of SMP was characterized using the established HPLC-UV protocol with PMP precolumn derivatization, and the results indicate that the polysaccharides are mainly composed of d-galactose (Gal), d-glucose (Glc) and d-galacturonic acid (GalUA), and their mole percentages are 64.5%, 31.1% and 4.4%, respectively.
29233711	3	12	with	protocol	437:444	arg1	derivatization					465:478	PMP precolumn derivatization	451:478	PMP precolumn derivatization	451:478	The monosaccharide composition of SMP was characterized using the established HPLC-UV protocol with PMP precolumn derivatization, and the results indicate that the polysaccharides are mainly composed of d-galactose (Gal), d-glucose (Glc) and d-galacturonic acid (GalUA), and their mole percentages are 64.5%, 31.1% and 4.4%, respectively.
29233711	2	13	theme	boiling	299:305	arg1	method					283:288	the method	279:288	the method of water boiling and ethanol precipitation with high purity	279:348	The polysaccharides were extracted by the method of water boiling and ethanol precipitation with high purity.
29233711	0	14	theme	carcinoma	123:131	arg1	cells					138:142	human colorectal carcinoma LoVo cells	106:142	human colorectal carcinoma LoVo cells	106:142	Antitumor effect and molecular mechanism of antioxidant polysaccharides from Salvia miltiorrhiza Bunge in human colorectal carcinoma LoVo cells.
29233711	2	15	theme	water	293:297	arg1	boiling					299:305	water boiling	293:305	water boiling	293:305	The polysaccharides were extracted by the method of water boiling and ethanol precipitation with high purity.
29233711	6	16	theme	LoVo	1052:1055	arg1	cells					1078:1082	typical tumor cells	1064:1082	typical tumor cells	1064:1082	Furthermore, SMP is observed with high inhibition ratio against LoVo cells (typical tumor cells) in both dose- and time-dependent manners.
29233711	6	16	theme	LoVo	1052:1055	arg1	cells					1057:1061	LoVo cells	1052:1061	LoVo cells (typical tumor cells)	1052:1083	Furthermore, SMP is observed with high inhibition ratio against LoVo cells (typical tumor cells) in both dose- and time-dependent manners.
29233711	7	17	theme	intracellular	1262:1274	arg1	pressure					1292:1299	the intracellular reactive oxygen pressure	1258:1299	the intracellular reactive oxygen pressure	1258:1299	FCM analysis demonstrates that SMP is able to induce apoptosis of LoVo cells, arrest the cell cycle at S phase, as well as elevate the intracellular reactive oxygen pressure.
29233711	0	18	theme	colorectal	112:121	arg1	cells					138:142	human colorectal carcinoma LoVo cells	106:142	human colorectal carcinoma LoVo cells	106:142	Antitumor effect and molecular mechanism of antioxidant polysaccharides from Salvia miltiorrhiza Bunge in human colorectal carcinoma LoVo cells.
29233711	3	19	theme	established	417:427	arg1	protocol					437:444	the established HPLC-UV protocol	413:444	the established HPLC-UV protocol with PMP precolumn derivatization	413:478	The monosaccharide composition of SMP was characterized using the established HPLC-UV protocol with PMP precolumn derivatization, and the results indicate that the polysaccharides are mainly composed of d-galactose (Gal), d-glucose (Glc) and d-galacturonic acid (GalUA), and their mole percentages are 64.5%, 31.1% and 4.4%, respectively.
29233711	6	20	theme	typical	1064:1070	arg1	cells					1078:1082	typical tumor cells	1064:1082	typical tumor cells	1064:1082	Furthermore, SMP is observed with high inhibition ratio against LoVo cells (typical tumor cells) in both dose- and time-dependent manners.
29233711	6	20	theme	typical	1064:1070	arg1	cells					1057:1061	LoVo cells	1052:1061	LoVo cells (typical tumor cells)	1052:1083	Furthermore, SMP is observed with high inhibition ratio against LoVo cells (typical tumor cells) in both dose- and time-dependent manners.
29233711	7	21	theme	reactive	1276:1283	arg1	pressure					1292:1299	the intracellular reactive oxygen pressure	1258:1299	the intracellular reactive oxygen pressure	1258:1299	FCM analysis demonstrates that SMP is able to induce apoptosis of LoVo cells, arrest the cell cycle at S phase, as well as elevate the intracellular reactive oxygen pressure.
29233711	0	22	from	mechanism	31:39	arg1	Bunge					97:101	Bunge	97:101	Bunge	97:101	Antitumor effect and molecular mechanism of antioxidant polysaccharides from Salvia miltiorrhiza Bunge in human colorectal carcinoma LoVo cells.
29233711	0	22	from	mechanism	31:39	arg1	cells					138:142	human colorectal carcinoma LoVo cells	106:142	human colorectal carcinoma LoVo cells	106:142	Antitumor effect and molecular mechanism of antioxidant polysaccharides from Salvia miltiorrhiza Bunge in human colorectal carcinoma LoVo cells.
29233711	8	23	theme	functional	1511:1520	arg1	development					1527:1537	functional food development	1511:1537	functional food development	1511:1537	These findings for the first time reveal the potential anti-tumor mechanism of SMP, suggesting that SMP may serve a natural anticancer agent with lower cost and cytotoxicity, as well as a bioactive factor for functional food development.
29233711	2	24	theme	high	338:341	arg1	purity					343:348	high purity	338:348	high purity	338:348	The polysaccharides were extracted by the method of water boiling and ethanol precipitation with high purity.
29233711	3	25	theme	precolumn	455:463	arg1	derivatization					465:478	PMP precolumn derivatization	451:478	PMP precolumn derivatization	451:478	The monosaccharide composition of SMP was characterized using the established HPLC-UV protocol with PMP precolumn derivatization, and the results indicate that the polysaccharides are mainly composed of d-galactose (Gal), d-glucose (Glc) and d-galacturonic acid (GalUA), and their mole percentages are 64.5%, 31.1% and 4.4%, respectively.
29233711	0	26	theme	Antitumor	0:8	arg1	effect					10:15	Antitumor effect	0:15	Antitumor effect	0:15	Antitumor effect and molecular mechanism of antioxidant polysaccharides from Salvia miltiorrhiza Bunge in human colorectal carcinoma LoVo cells.
29233711	6	27	located	observed	1008:1015	arg2	SMP					1001:1003	SMP	1001:1003	SMP	1001:1003	Furthermore, SMP is observed with high inhibition ratio against LoVo cells (typical tumor cells) in both dose- and time-dependent manners.
29233711	6	27	located	observed	1008:1015	arg1	dose-					1093:1097	dose-	1093:1097	dose-	1093:1097	Furthermore, SMP is observed with high inhibition ratio against LoVo cells (typical tumor cells) in both dose- and time-dependent manners.
29233711	6	27	located	observed	1008:1015	arg1	manners					1118:1124	time-dependent manners	1103:1124	time-dependent manners	1103:1124	Furthermore, SMP is observed with high inhibition ratio against LoVo cells (typical tumor cells) in both dose- and time-dependent manners.
29233711	0	28	theme	molecular	21:29	arg1	mechanism					31:39	molecular mechanism	21:39	molecular mechanism	21:39	Antitumor effect and molecular mechanism of antioxidant polysaccharides from Salvia miltiorrhiza Bunge in human colorectal carcinoma LoVo cells.
29233711	8	29	theme	SMP	1381:1383	arg1	mechanism					1368:1376	the potential anti-tumor mechanism	1343:1376	the potential anti-tumor mechanism of SMP	1343:1383	These findings for the first time reveal the potential anti-tumor mechanism of SMP, suggesting that SMP may serve a natural anticancer agent with lower cost and cytotoxicity, as well as a bioactive factor for functional food development.
29233711	8	30	with	agent	1437:1441	arg1	cytotoxicity					1463:1474	cytotoxicity	1463:1474	cytotoxicity	1463:1474	These findings for the first time reveal the potential anti-tumor mechanism of SMP, suggesting that SMP may serve a natural anticancer agent with lower cost and cytotoxicity, as well as a bioactive factor for functional food development.
29233711	8	30	with	agent	1437:1441	arg1	cost					1454:1457	lower cost	1448:1457	lower cost	1448:1457	These findings for the first time reveal the potential anti-tumor mechanism of SMP, suggesting that SMP may serve a natural anticancer agent with lower cost and cytotoxicity, as well as a bioactive factor for functional food development.
29233711	2	31	theme	precipitation	319:331	arg1	method					283:288	the method	279:288	the method of water boiling and ethanol precipitation with high purity	279:348	The polysaccharides were extracted by the method of water boiling and ethanol precipitation with high purity.
29233711	5	32	contain	possess	919:925	arg1	polysaccharides					876:890	polysaccharides	876:890	polysaccharides from S. miltiorrhiza Bunge	876:917	The results indicate that polysaccharides from S. miltiorrhiza Bunge possess versatile antioxidant activities in a dose-dependent manner.
29233711	5	32	contain	possess	919:925	arg2	activities					949:958	versatile antioxidant activities	927:958	versatile antioxidant activities	927:958	The results indicate that polysaccharides from S. miltiorrhiza Bunge possess versatile antioxidant activities in a dose-dependent manner.
29233711	0	33	theme	LoVo	133:136	arg1	cells					138:142	human colorectal carcinoma LoVo cells	106:142	human colorectal carcinoma LoVo cells	106:142	Antitumor effect and molecular mechanism of antioxidant polysaccharides from Salvia miltiorrhiza Bunge in human colorectal carcinoma LoVo cells.
29233711	0	34	from	Bunge	97:101	arg1	effect					10:15	Antitumor effect	0:15	Antitumor effect	0:15	Antitumor effect and molecular mechanism of antioxidant polysaccharides from Salvia miltiorrhiza Bunge in human colorectal carcinoma LoVo cells.
29233711	0	34	from	Bunge	97:101	arg1	mechanism					31:39	molecular mechanism	21:39	molecular mechanism	21:39	Antitumor effect and molecular mechanism of antioxidant polysaccharides from Salvia miltiorrhiza Bunge in human colorectal carcinoma LoVo cells.
29233711	0	34	from	Bunge	97:101	arg1	polysaccharides					56:70	antioxidant polysaccharides	44:70	antioxidant polysaccharides from Salvia miltiorrhiza Bunge	44:101	Antitumor effect and molecular mechanism of antioxidant polysaccharides from Salvia miltiorrhiza Bunge in human colorectal carcinoma LoVo cells.
29233711	2	35	theme	ethanol	311:317	arg1	precipitation					319:331	ethanol precipitation	311:331	ethanol precipitation	311:331	The polysaccharides were extracted by the method of water boiling and ethanol precipitation with high purity.
29233711	6	36	theme	inhibition	1027:1036	arg1	ratio					1038:1042	high inhibition ratio	1022:1042	high inhibition ratio against LoVo cells (typical tumor cells)	1022:1083	Furthermore, SMP is observed with high inhibition ratio against LoVo cells (typical tumor cells) in both dose- and time-dependent manners.
29233711	6	37	theme	high	1022:1025	arg1	ratio					1038:1042	high inhibition ratio	1022:1042	high inhibition ratio against LoVo cells (typical tumor cells)	1022:1083	Furthermore, SMP is observed with high inhibition ratio against LoVo cells (typical tumor cells) in both dose- and time-dependent manners.
29233711	3	38	theme	PMP	451:453	arg1	derivatization					465:478	PMP precolumn derivatization	451:478	PMP precolumn derivatization	451:478	The monosaccharide composition of SMP was characterized using the established HPLC-UV protocol with PMP precolumn derivatization, and the results indicate that the polysaccharides are mainly composed of d-galactose (Gal), d-glucose (Glc) and d-galacturonic acid (GalUA), and their mole percentages are 64.5%, 31.1% and 4.4%, respectively.
29233711	7	39	theme	FCM	1127:1129	arg1	analysis					1131:1138	FCM analysis	1127:1138	FCM analysis	1127:1138	FCM analysis demonstrates that SMP is able to induce apoptosis of LoVo cells, arrest the cell cycle at S phase, as well as elevate the intracellular reactive oxygen pressure.
29233711	3	40	theme	mole	632:635	arg1	percentages					637:647	their mole percentages	626:647	their mole percentages	626:647	The monosaccharide composition of SMP was characterized using the established HPLC-UV protocol with PMP precolumn derivatization, and the results indicate that the polysaccharides are mainly composed of d-galactose (Gal), d-glucose (Glc) and d-galacturonic acid (GalUA), and their mole percentages are 64.5%, 31.1% and 4.4%, respectively.
29233711	3	40	theme	mole	632:635	arg1	%					657:657	64.5%	653:657	64.5%	653:657	The monosaccharide composition of SMP was characterized using the established HPLC-UV protocol with PMP precolumn derivatization, and the results indicate that the polysaccharides are mainly composed of d-galactose (Gal), d-glucose (Glc) and d-galacturonic acid (GalUA), and their mole percentages are 64.5%, 31.1% and 4.4%, respectively.
29233711	8	41	with	factor	1500:1505	arg1	cytotoxicity					1463:1474	cytotoxicity	1463:1474	cytotoxicity	1463:1474	These findings for the first time reveal the potential anti-tumor mechanism of SMP, suggesting that SMP may serve a natural anticancer agent with lower cost and cytotoxicity, as well as a bioactive factor for functional food development.
29233711	8	41	with	factor	1500:1505	arg1	cost					1454:1457	lower cost	1448:1457	lower cost	1448:1457	These findings for the first time reveal the potential anti-tumor mechanism of SMP, suggesting that SMP may serve a natural anticancer agent with lower cost and cytotoxicity, as well as a bioactive factor for functional food development.
29233711	0	42	theme	polysaccharides	56:70	arg1	effect					10:15	Antitumor effect	0:15	Antitumor effect	0:15	Antitumor effect and molecular mechanism of antioxidant polysaccharides from Salvia miltiorrhiza Bunge in human colorectal carcinoma LoVo cells.
29233711	0	42	theme	polysaccharides	56:70	arg1	mechanism					31:39	molecular mechanism	21:39	molecular mechanism	21:39	Antitumor effect and molecular mechanism of antioxidant polysaccharides from Salvia miltiorrhiza Bunge in human colorectal carcinoma LoVo cells.
29233711	3	43	theme	SMP	385:387	arg1	composition					370:380	The monosaccharide composition	351:380	The monosaccharide composition of SMP	351:387	The monosaccharide composition of SMP was characterized using the established HPLC-UV protocol with PMP precolumn derivatization, and the results indicate that the polysaccharides are mainly composed of d-galactose (Gal), d-glucose (Glc) and d-galacturonic acid (GalUA), and their mole percentages are 64.5%, 31.1% and 4.4%, respectively.
29233711	7	44	theme	cells	1198:1202	arg1	apoptosis					1180:1188	apoptosis	1180:1188	apoptosis of LoVo cells	1180:1202	FCM analysis demonstrates that SMP is able to induce apoptosis of LoVo cells, arrest the cell cycle at S phase, as well as elevate the intracellular reactive oxygen pressure.
29233711	3	45	theme	d-galacturonic	593:606	arg1	GalUA					614:618	GalUA	614:618	GalUA	614:618	The monosaccharide composition of SMP was characterized using the established HPLC-UV protocol with PMP precolumn derivatization, and the results indicate that the polysaccharides are mainly composed of d-galactose (Gal), d-glucose (Glc) and d-galacturonic acid (GalUA), and their mole percentages are 64.5%, 31.1% and 4.4%, respectively.
29233711	3	45	theme	d-galacturonic	593:606	arg1	acid					608:611	d-galacturonic acid	593:611	d-galacturonic acid (GalUA)	593:619	The monosaccharide composition of SMP was characterized using the established HPLC-UV protocol with PMP precolumn derivatization, and the results indicate that the polysaccharides are mainly composed of d-galactose (Gal), d-glucose (Glc) and d-galacturonic acid (GalUA), and their mole percentages are 64.5%, 31.1% and 4.4%, respectively.
29233711	7	46	theme	S	1230:1230	arg1	phase					1232:1236	S phase	1230:1236	S phase	1230:1236	FCM analysis demonstrates that SMP is able to induce apoptosis of LoVo cells, arrest the cell cycle at S phase, as well as elevate the intracellular reactive oxygen pressure.
29233711	0	47	theme	antioxidant	44:54	arg1	polysaccharides					56:70	antioxidant polysaccharides	44:70	antioxidant polysaccharides from Salvia miltiorrhiza Bunge	44:101	Antitumor effect and molecular mechanism of antioxidant polysaccharides from Salvia miltiorrhiza Bunge in human colorectal carcinoma LoVo cells.
29233711	2	48	with	method	283:288	arg1	purity					343:348	high purity	338:348	high purity	338:348	The polysaccharides were extracted by the method of water boiling and ethanol precipitation with high purity.
29233711	4	49	theme	scavenging	778:787	arg1	power					771:775	power	771:775	power	771:775	In addition, the antioxidant potential of SMP was evaluated in terms of reducing power, scavenging ability against DPPH, superoxide and hydroxyl free radicals.
29233711	4	49	theme	scavenging	778:787	arg1	ability					789:795	scavenging ability	778:795	scavenging ability	778:795	In addition, the antioxidant potential of SMP was evaluated in terms of reducing power, scavenging ability against DPPH, superoxide and hydroxyl free radicals.
29233711	7	50	theme	cell	1216:1219	arg1	cycle					1221:1225	the cell cycle	1212:1225	the cell cycle	1212:1225	FCM analysis demonstrates that SMP is able to induce apoptosis of LoVo cells, arrest the cell cycle at S phase, as well as elevate the intracellular reactive oxygen pressure.
29233711	5	51	theme	dose-dependent	965:978	arg1	manner					980:985	a dose-dependent manner	963:985	a dose-dependent manner	963:985	The results indicate that polysaccharides from S. miltiorrhiza Bunge possess versatile antioxidant activities in a dose-dependent manner.
29233711	8	52	theme	first	1325:1329	arg1	time					1331:1334	the first time	1321:1334	the first time	1321:1334	These findings for the first time reveal the potential anti-tumor mechanism of SMP, suggesting that SMP may serve a natural anticancer agent with lower cost and cytotoxicity, as well as a bioactive factor for functional food development.
29233711	8	53	theme	natural	1418:1424	arg1	agent					1437:1441	a natural anticancer agent	1416:1441	a natural anticancer agent with lower cost and cytotoxicity	1416:1474	These findings for the first time reveal the potential anti-tumor mechanism of SMP, suggesting that SMP may serve a natural anticancer agent with lower cost and cytotoxicity, as well as a bioactive factor for functional food development.
29233711	5	54	theme	antioxidant	937:947	arg1	activities					949:958	versatile antioxidant activities	927:958	versatile antioxidant activities	927:958	The results indicate that polysaccharides from S. miltiorrhiza Bunge possess versatile antioxidant activities in a dose-dependent manner.
29233711	1	55	theme	Salvia	145:150	arg1	SMP					188:190	SMP	188:190	SMP	188:190	Salvia miltiorrhiza Bunge polysaccharides (SMP) was comprehensively investigated in this study.
29233711	1	55	theme	Salvia	145:150	arg1	polysaccharides					171:185	Salvia miltiorrhiza Bunge polysaccharides	145:185	Salvia miltiorrhiza Bunge polysaccharides (SMP)	145:191	Salvia miltiorrhiza Bunge polysaccharides (SMP) was comprehensively investigated in this study.
29233711	8	56	theme	food	1522:1525	arg1	development					1527:1537	functional food development	1511:1537	functional food development	1511:1537	These findings for the first time reveal the potential anti-tumor mechanism of SMP, suggesting that SMP may serve a natural anticancer agent with lower cost and cytotoxicity, as well as a bioactive factor for functional food development.
29233711	7	57	theme	LoVo	1193:1196	arg1	cells					1198:1202	LoVo cells	1193:1202	LoVo cells	1193:1202	FCM analysis demonstrates that SMP is able to induce apoptosis of LoVo cells, arrest the cell cycle at S phase, as well as elevate the intracellular reactive oxygen pressure.
29233711	7	58	theme	oxygen	1285:1290	arg1	pressure					1292:1299	the intracellular reactive oxygen pressure	1258:1299	the intracellular reactive oxygen pressure	1258:1299	FCM analysis demonstrates that SMP is able to induce apoptosis of LoVo cells, arrest the cell cycle at S phase, as well as elevate the intracellular reactive oxygen pressure.
29233711	1	59	theme	miltiorrhiza	152:163	arg1	SMP					188:190	SMP	188:190	SMP	188:190	Salvia miltiorrhiza Bunge polysaccharides (SMP) was comprehensively investigated in this study.
29233711	1	59	theme	miltiorrhiza	152:163	arg1	polysaccharides					171:185	Salvia miltiorrhiza Bunge polysaccharides	145:185	Salvia miltiorrhiza Bunge polysaccharides (SMP)	145:191	Salvia miltiorrhiza Bunge polysaccharides (SMP) was comprehensively investigated in this study.
29233711	5	60	from	Bunge	913:917	arg1	polysaccharides					876:890	polysaccharides	876:890	polysaccharides from S. miltiorrhiza Bunge	876:917	The results indicate that polysaccharides from S. miltiorrhiza Bunge possess versatile antioxidant activities in a dose-dependent manner.
29233711	3	61	theme	monosaccharide	355:368	arg1	composition					370:380	The monosaccharide composition	351:380	The monosaccharide composition of SMP	351:387	The monosaccharide composition of SMP was characterized using the established HPLC-UV protocol with PMP precolumn derivatization, and the results indicate that the polysaccharides are mainly composed of d-galactose (Gal), d-glucose (Glc) and d-galacturonic acid (GalUA), and their mole percentages are 64.5%, 31.1% and 4.4%, respectively.
29233711	6	62	theme	time-dependent	1103:1116	arg1	manners					1118:1124	time-dependent manners	1103:1124	time-dependent manners	1103:1124	Furthermore, SMP is observed with high inhibition ratio against LoVo cells (typical tumor cells) in both dose- and time-dependent manners.
29233711	1	63	theme	Bunge	165:169	arg1	SMP					188:190	SMP	188:190	SMP	188:190	Salvia miltiorrhiza Bunge polysaccharides (SMP) was comprehensively investigated in this study.
29233711	1	63	theme	Bunge	165:169	arg1	polysaccharides					171:185	Salvia miltiorrhiza Bunge polysaccharides	145:185	Salvia miltiorrhiza Bunge polysaccharides (SMP)	145:191	Salvia miltiorrhiza Bunge polysaccharides (SMP) was comprehensively investigated in this study.
29233711	8	64	theme	lower	1448:1452	arg1	cost					1454:1457	lower cost	1448:1457	lower cost	1448:1457	These findings for the first time reveal the potential anti-tumor mechanism of SMP, suggesting that SMP may serve a natural anticancer agent with lower cost and cytotoxicity, as well as a bioactive factor for functional food development.
29233711	8	65	theme	bioactive	1490:1498	arg1	factor					1500:1505	a bioactive factor	1488:1505	a bioactive factor for functional food development	1488:1537	These findings for the first time reveal the potential anti-tumor mechanism of SMP, suggesting that SMP may serve a natural anticancer agent with lower cost and cytotoxicity, as well as a bioactive factor for functional food development.
24875633	9	0	dep	RESULTS	1155:1161	arg1	indicate					1172:1179	indicate	1172:1179	indicate that FCSPs are tightly associated with proteins and cellulose within the walls	1172:1258	KEY RESULTS The data indicate that FCSPs are tightly associated with proteins and cellulose within the walls.
24875633	6	1	theme	wall	925:928	arg1	partners					930:937	cell wall partners	920:937	cell wall partners	920:937	Dedicated enzymatic degradations were used to release and identify cell wall partners.
24875633	4	2	theme	biosynthetic	591:602	arg1	routes					604:609	the corresponding biosynthetic routes	573:609	the corresponding biosynthetic routes	573:609	Recent molecular analysis of the corresponding biosynthetic routes points toward a complex evolutionary history that shaped the ECM structure in brown algae.
24875633	13	3	theme	sulfated	1818:1825	arg1	polysaccharides					1827:1841	sulfated polysaccharides	1818:1841	sulfated polysaccharides	1818:1841	Enzymatic cross-linking of alginates by phenols may regulate the strengthening of the wall, and sulfated polysaccharides may play a key role in the adaptation to osmotic stress.
24875633	4	4	from	structure	676:684	arg1	algae					695:699	brown algae	689:699	brown algae	689:699	Recent molecular analysis of the corresponding biosynthetic routes points toward a complex evolutionary history that shaped the ECM structure in brown algae.
24875633	5	5	theme	order	823:827	arg1	Fucales					829:835	the order Fucales	819:835	the order Fucales	819:835	METHODS Exhaustive sequential extractions and composition analyses of cell wall material from various brown algae of the order Fucales were performed.
24875633	1	6	theme	Brown	157:161	arg1	algae					163:167	Brown algae	157:167	Brown algae	157:167	BACKGROUND AND AIMS Brown algae are photosynthetic multicellular marine organisms evolutionarily distant from land plants, with a distinctive cell wall.
24875633	12	7	theme	architecture	1583:1594	arg1	snapshot					1557:1564	a global snapshot	1548:1564	a global snapshot of the cell wall architecture in brown algae	1548:1609	CONCLUSIONS The data provide a global snapshot of the cell wall architecture in brown algae, and contribute to the understanding of the structure-function relationships of the main cell wall components.
24875633	8	8	theme	additional	1050:1059	arg1	assessment					1072:1081	An additional structural assessment	1047:1081	An additional structural assessment of the sulfated fucan extracted from Himanthalia elongata	1047:1139	An additional structural assessment of the sulfated fucan extracted from Himanthalia elongata was made.
24875633	4	9	theme	evolutionary	635:646	arg1	history					648:654	a complex evolutionary history	625:654	a complex evolutionary history that shaped the ECM structure in brown algae	625:699	Recent molecular analysis of the corresponding biosynthetic routes points toward a complex evolutionary history that shaped the ECM structure in brown algae.
24875633	11	10	theme	regular	1374:1380	arg1	structure					1399:1407	a regular α-(1→3) backbone structure	1372:1407	a regular α-(1→3) backbone structure	1372:1407	The sulfated fucans from H. elongata were shown to have a regular α-(1→3) backbone structure, while an alternating α-(1→3), (1→4) structure has been described in some brown algae from the order Fucales.
24875633	5	11	theme	Exhaustive	710:719	arg1	extractions					732:742	Exhaustive sequential extractions	710:742	Exhaustive sequential extractions	710:742	METHODS Exhaustive sequential extractions and composition analyses of cell wall material from various brown algae of the order Fucales were performed.
24875633	5	12	from	analyses	760:767	arg1	algae					810:814	various brown algae	796:814	various brown algae of the order Fucales	796:835	METHODS Exhaustive sequential extractions and composition analyses of cell wall material from various brown algae of the order Fucales were performed.
24875633	1	13	theme	multicellular	188:200	arg1	AIMS					152:155	AIMS	152:155	AIMS	152:155	BACKGROUND AND AIMS Brown algae are photosynthetic multicellular marine organisms evolutionarily distant from land plants, with a distinctive cell wall.
24875633	1	13	theme	multicellular	188:200	arg1	BACKGROUND					137:146	BACKGROUND	137:146	BACKGROUND	137:146	BACKGROUND AND AIMS Brown algae are photosynthetic multicellular marine organisms evolutionarily distant from land plants, with a distinctive cell wall.
24875633	1	13	theme	multicellular	188:200	arg1	organisms					209:217	photosynthetic multicellular marine organisms	173:217	photosynthetic multicellular marine organisms evolutionarily distant from land plants	173:257	BACKGROUND AND AIMS Brown algae are photosynthetic multicellular marine organisms evolutionarily distant from land plants, with a distinctive cell wall.
24875633	12	14	theme	brown	1599:1603	arg1	algae					1605:1609	brown algae	1599:1609	brown algae	1599:1609	CONCLUSIONS The data provide a global snapshot of the cell wall architecture in brown algae, and contribute to the understanding of the structure-function relationships of the main cell wall components.
24875633	10	15	theme	phenolic	1296:1303	arg1	compounds					1305:1313	most phenolic compounds	1291:1313	most phenolic compounds	1291:1313	Alginates are associated with most phenolic compounds.
24875633	0	16	from	Fucales	56:62	arg1	fractionation					23:35	Chemical and enzymatic fractionation	0:35	Chemical and enzymatic fractionation of cell walls from Fucales: insights into the structure of the extracellular matrix of brown algae.	0:135	Chemical and enzymatic fractionation of cell walls from Fucales: insights into the structure of the extracellular matrix of brown algae.
24875633	0	17	theme	algae	130:134	arg1	matrix					114:119	the extracellular matrix	96:119	the extracellular matrix of brown algae	96:134	Chemical and enzymatic fractionation of cell walls from Fucales: insights into the structure of the extracellular matrix of brown algae.
24875633	12	18	theme	cell	1700:1703	arg1	components					1710:1719	the main cell wall components	1691:1719	the main cell wall components	1691:1719	CONCLUSIONS The data provide a global snapshot of the cell wall architecture in brown algae, and contribute to the understanding of the structure-function relationships of the main cell wall components.
24875633	12	19	theme	cell	1573:1576	arg1	architecture					1583:1594	the cell wall architecture	1569:1594	the cell wall architecture	1569:1594	CONCLUSIONS The data provide a global snapshot of the cell wall architecture in brown algae, and contribute to the understanding of the structure-function relationships of the main cell wall components.
24875633	10	20	theme	most	1291:1294	arg1	compounds					1305:1313	most phenolic compounds	1291:1313	most phenolic compounds	1291:1313	Alginates are associated with most phenolic compounds.
24875633	12	21	theme	components	1710:1719	arg1	relationships					1674:1686	the structure-function relationships	1651:1686	the structure-function relationships of the main cell wall components	1651:1719	CONCLUSIONS The data provide a global snapshot of the cell wall architecture in brown algae, and contribute to the understanding of the structure-function relationships of the main cell wall components.
24875633	4	22	theme	brown	689:693	arg1	algae					695:699	brown algae	689:699	brown algae	689:699	Recent molecular analysis of the corresponding biosynthetic routes points toward a complex evolutionary history that shaped the ECM structure in brown algae.
24875633	5	23	theme	wall	777:780	arg1	material					782:789	cell wall material	772:789	cell wall material from various brown algae of the order Fucales	772:835	METHODS Exhaustive sequential extractions and composition analyses of cell wall material from various brown algae of the order Fucales were performed.
24875633	5	24	from	algae	810:814	arg1	analyses					760:767	composition analyses	748:767	composition analyses	748:767	METHODS Exhaustive sequential extractions and composition analyses of cell wall material from various brown algae of the order Fucales were performed.
24875633	5	24	from	algae	810:814	arg1	material					782:789	cell wall material	772:789	cell wall material from various brown algae of the order Fucales	772:835	METHODS Exhaustive sequential extractions and composition analyses of cell wall material from various brown algae of the order Fucales were performed.
24875633	5	24	from	algae	810:814	arg1	METHODS					702:708	METHODS	702:708	METHODS	702:708	METHODS Exhaustive sequential extractions and composition analyses of cell wall material from various brown algae of the order Fucales were performed.
24875633	12	25	theme	relationships	1674:1686	arg1	understanding					1634:1646	the understanding	1630:1646	the understanding of the structure-function relationships of the main cell wall components	1630:1719	CONCLUSIONS The data provide a global snapshot of the cell wall architecture in brown algae, and contribute to the understanding of the structure-function relationships of the main cell wall components.
24875633	12	26	theme	global	1550:1555	arg1	snapshot					1557:1564	a global snapshot	1548:1564	a global snapshot of the cell wall architecture in brown algae	1548:1609	CONCLUSIONS The data provide a global snapshot of the cell wall architecture in brown algae, and contribute to the understanding of the structure-function relationships of the main cell wall components.
24875633	5	27	dep	METHODS	702:708	arg1	extractions					732:742	Exhaustive sequential extractions	710:742	Exhaustive sequential extractions	710:742	METHODS Exhaustive sequential extractions and composition analyses of cell wall material from various brown algae of the order Fucales were performed.
24875633	0	28	theme	brown	124:128	arg1	algae					130:134	brown algae	124:134	brown algae	124:134	Chemical and enzymatic fractionation of cell walls from Fucales: insights into the structure of the extracellular matrix of brown algae.
24875633	13	29	theme	osmotic	1884:1890	arg1	stress					1892:1897	osmotic stress	1884:1897	osmotic stress	1884:1897	Enzymatic cross-linking of alginates by phenols may regulate the strengthening of the wall, and sulfated polysaccharides may play a key role in the adaptation to osmotic stress.
24875633	5	30	theme	composition	748:758	arg1	analyses					760:767	composition analyses	748:767	composition analyses	748:767	METHODS Exhaustive sequential extractions and composition analyses of cell wall material from various brown algae of the order Fucales were performed.
24875633	7	31	theme	systematic	974:983	arg1	analysis					1001:1008	systematic chromatographic analysis	974:1008	systematic chromatographic analysis to study polymer interlinks further	974:1044	This approach was complemented by systematic chromatographic analysis to study polymer interlinks further.
24875633	14	32	theme	multicellularity	1999:2014	arg1	evolution					1986:1994	the evolution	1982:1994	the evolution of multicellularity in brown algae	1982:2029	The emergence and evolution of ECM components is further discussed in relation to the evolution of multicellularity in brown algae.
24875633	13	33	theme	key	1854:1856	arg1	role					1858:1861	a key role	1852:1861	a key role	1852:1861	Enzymatic cross-linking of alginates by phenols may regulate the strengthening of the wall, and sulfated polysaccharides may play a key role in the adaptation to osmotic stress.
24875633	0	34	theme	cell	40:43	arg1	walls					45:49	cell walls	40:49	cell walls	40:49	Chemical and enzymatic fractionation of cell walls from Fucales: insights into the structure of the extracellular matrix of brown algae.
24875633	11	35	from	Fucales	1510:1516	arg1	algae					1489:1493	some brown algae	1478:1493	some brown algae from the order Fucales	1478:1516	The sulfated fucans from H. elongata were shown to have a regular α-(1→3) backbone structure, while an alternating α-(1→3), (1→4) structure has been described in some brown algae from the order Fucales.
24875633	1	36	dep	BACKGROUND	137:146	arg1	algae					163:167	Brown algae	157:167	Brown algae	157:167	BACKGROUND AND AIMS Brown algae are photosynthetic multicellular marine organisms evolutionarily distant from land plants, with a distinctive cell wall.
24875633	2	37	theme	sulfated	376:383	arg1	polysaccharides					385:399	fucose-containing sulfated polysaccharides	358:399	fucose-containing sulfated polysaccharides	358:399	They feature carbohydrates shared with plants (cellulose), animals (fucose-containing sulfated polysaccharides, FCSPs) or bacteria (alginates).
24875633	2	37	theme	sulfated	376:383	arg1	FCSPs					402:406	FCSPs	402:406	FCSPs	402:406	They feature carbohydrates shared with plants (cellulose), animals (fucose-containing sulfated polysaccharides, FCSPs) or bacteria (alginates).
24875633	0	38	theme	Chemical	0:7	arg1	fractionation					23:35	Chemical and enzymatic fractionation	0:35	Chemical and enzymatic fractionation of cell walls from Fucales: insights into the structure of the extracellular matrix of brown algae.	0:135	Chemical and enzymatic fractionation of cell walls from Fucales: insights into the structure of the extracellular matrix of brown algae.
24875633	1	39	theme	distinctive	267:277	arg1	wall					284:287	a distinctive cell wall	265:287	a distinctive cell wall	265:287	BACKGROUND AND AIMS Brown algae are photosynthetic multicellular marine organisms evolutionarily distant from land plants, with a distinctive cell wall.
24875633	11	40	dep	H.	1341:1342	arg1	elongata					1344:1351	H. elongata	1341:1351	H. elongata	1341:1351	The sulfated fucans from H. elongata were shown to have a regular α-(1→3) backbone structure, while an alternating α-(1→3), (1→4) structure has been described in some brown algae from the order Fucales.
24875633	0	41	theme	enzymatic	13:21	arg1	fractionation					23:35	Chemical and enzymatic fractionation	0:35	Chemical and enzymatic fractionation of cell walls from Fucales: insights into the structure of the extracellular matrix of brown algae.	0:135	Chemical and enzymatic fractionation of cell walls from Fucales: insights into the structure of the extracellular matrix of brown algae.
24875633	1	42	with	organisms	209:217	arg1	wall					284:287	a distinctive cell wall	265:287	a distinctive cell wall	265:287	BACKGROUND AND AIMS Brown algae are photosynthetic multicellular marine organisms evolutionarily distant from land plants, with a distinctive cell wall.
24875633	4	43	theme	molecular	551:559	arg1	analysis					561:568	Recent molecular analysis	544:568	Recent molecular analysis of the corresponding biosynthetic routes	544:609	Recent molecular analysis of the corresponding biosynthetic routes points toward a complex evolutionary history that shaped the ECM structure in brown algae.
24875633	14	44	dep	emergence	1904:1912	arg1	The					1900:1902	The	1900:1902	The	1900:1902	The emergence and evolution of ECM components is further discussed in relation to the evolution of multicellularity in brown algae.
24875633	5	45	theme	brown	804:808	arg1	algae					810:814	various brown algae	796:814	various brown algae of the order Fucales	796:835	METHODS Exhaustive sequential extractions and composition analyses of cell wall material from various brown algae of the order Fucales were performed.
24875633	0	46	theme	walls	45:49	arg1	fractionation					23:35	Chemical and enzymatic fractionation	0:35	Chemical and enzymatic fractionation of cell walls from Fucales: insights into the structure of the extracellular matrix of brown algae.	0:135	Chemical and enzymatic fractionation of cell walls from Fucales: insights into the structure of the extracellular matrix of brown algae.
24875633	11	47	from	H.	1341:1342	arg1	fucans					1329:1334	The sulfated fucans	1316:1334	The sulfated fucans from H. elongata	1316:1351	The sulfated fucans from H. elongata were shown to have a regular α-(1→3) backbone structure, while an alternating α-(1→3), (1→4) structure has been described in some brown algae from the order Fucales.
24875633	6	48	theme	enzymatic	863:871	arg1	degradations					873:884	Dedicated enzymatic degradations	853:884	Dedicated enzymatic degradations	853:884	Dedicated enzymatic degradations were used to release and identify cell wall partners.
24875633	14	49	theme	components	1935:1944	arg1	evolution					1918:1926	evolution	1918:1926	evolution	1918:1926	The emergence and evolution of ECM components is further discussed in relation to the evolution of multicellularity in brown algae.
24875633	14	49	theme	components	1935:1944	arg1	emergence					1904:1912	emergence	1904:1912	emergence	1904:1912	The emergence and evolution of ECM components is further discussed in relation to the evolution of multicellularity in brown algae.
24875633	4	50	theme	routes	604:609	arg1	analysis					561:568	Recent molecular analysis	544:568	Recent molecular analysis of the corresponding biosynthetic routes	544:609	Recent molecular analysis of the corresponding biosynthetic routes points toward a complex evolutionary history that shaped the ECM structure in brown algae.
24875633	8	51	dep	Himanthalia	1120:1130	arg1	elongata					1132:1139	Himanthalia elongata	1120:1139	Himanthalia elongata	1120:1139	An additional structural assessment of the sulfated fucan extracted from Himanthalia elongata was made.
24875633	13	52	theme	alginates	1749:1757	arg1	cross-linking					1732:1744	Enzymatic cross-linking	1722:1744	Enzymatic cross-linking of alginates by phenols	1722:1768	Enzymatic cross-linking of alginates by phenols may regulate the strengthening of the wall, and sulfated polysaccharides may play a key role in the adaptation to osmotic stress.
24875633	3	53	theme	extracellular	494:506	arg1	matrix					508:513	a three-dimensional extracellular matrix	474:513	a three-dimensional extracellular matrix (ECM)	474:519	How these components are organized into a three-dimensional extracellular matrix (ECM) still remains unclear.
24875633	3	53	theme	extracellular	494:506	arg1	ECM					516:518	ECM	516:518	ECM	516:518	How these components are organized into a three-dimensional extracellular matrix (ECM) still remains unclear.
24875633	5	54	from	METHODS	702:708	arg1	algae					810:814	various brown algae	796:814	various brown algae of the order Fucales	796:835	METHODS Exhaustive sequential extractions and composition analyses of cell wall material from various brown algae of the order Fucales were performed.
24875633	6	55	theme	cell	920:923	arg1	partners					930:937	cell wall partners	920:937	cell wall partners	920:937	Dedicated enzymatic degradations were used to release and identify cell wall partners.
24875633	4	56	theme	corresponding	577:589	arg1	routes					604:609	the corresponding biosynthetic routes	573:609	the corresponding biosynthetic routes	573:609	Recent molecular analysis of the corresponding biosynthetic routes points toward a complex evolutionary history that shaped the ECM structure in brown algae.
24875633	11	57	theme	α-	1382:1383	arg1	structure					1399:1407	a regular α-(1→3) backbone structure	1372:1407	a regular α-(1→3) backbone structure	1372:1407	The sulfated fucans from H. elongata were shown to have a regular α-(1→3) backbone structure, while an alternating α-(1→3), (1→4) structure has been described in some brown algae from the order Fucales.
24875633	8	58	theme	structural	1061:1070	arg1	assessment					1072:1081	An additional structural assessment	1047:1081	An additional structural assessment of the sulfated fucan extracted from Himanthalia elongata	1047:1139	An additional structural assessment of the sulfated fucan extracted from Himanthalia elongata was made.
24875633	4	59	theme	ECM	672:674	arg1	structure					676:684	the ECM structure	668:684	the ECM structure in brown algae	668:699	Recent molecular analysis of the corresponding biosynthetic routes points toward a complex evolutionary history that shaped the ECM structure in brown algae.
24875633	0	60	theme	matrix	114:119	arg1	structure					83:91	the structure	79:91	the structure of the extracellular matrix of brown algae	79:134	Chemical and enzymatic fractionation of cell walls from Fucales: insights into the structure of the extracellular matrix of brown algae.
24875633	1	61	theme	photosynthetic	173:186	arg1	AIMS					152:155	AIMS	152:155	AIMS	152:155	BACKGROUND AND AIMS Brown algae are photosynthetic multicellular marine organisms evolutionarily distant from land plants, with a distinctive cell wall.
24875633	1	61	theme	photosynthetic	173:186	arg1	BACKGROUND					137:146	BACKGROUND	137:146	BACKGROUND	137:146	BACKGROUND AND AIMS Brown algae are photosynthetic multicellular marine organisms evolutionarily distant from land plants, with a distinctive cell wall.
24875633	1	61	theme	photosynthetic	173:186	arg1	organisms					209:217	photosynthetic multicellular marine organisms	173:217	photosynthetic multicellular marine organisms evolutionarily distant from land plants	173:257	BACKGROUND AND AIMS Brown algae are photosynthetic multicellular marine organisms evolutionarily distant from land plants, with a distinctive cell wall.
24875633	4	62	theme	complex	627:633	arg1	history					648:654	a complex evolutionary history	625:654	a complex evolutionary history that shaped the ECM structure in brown algae	625:699	Recent molecular analysis of the corresponding biosynthetic routes points toward a complex evolutionary history that shaped the ECM structure in brown algae.
24875633	11	63	theme	backbone	1390:1397	arg1	structure					1399:1407	a regular α-(1→3) backbone structure	1372:1407	a regular α-(1→3) backbone structure	1372:1407	The sulfated fucans from H. elongata were shown to have a regular α-(1→3) backbone structure, while an alternating α-(1→3), (1→4) structure has been described in some brown algae from the order Fucales.
24875633	5	64	theme	sequential	721:730	arg1	extractions					732:742	Exhaustive sequential extractions	710:742	Exhaustive sequential extractions	710:742	METHODS Exhaustive sequential extractions and composition analyses of cell wall material from various brown algae of the order Fucales were performed.
24875633	1	65	theme	marine	202:207	arg1	AIMS					152:155	AIMS	152:155	AIMS	152:155	BACKGROUND AND AIMS Brown algae are photosynthetic multicellular marine organisms evolutionarily distant from land plants, with a distinctive cell wall.
24875633	1	65	theme	marine	202:207	arg1	BACKGROUND					137:146	BACKGROUND	137:146	BACKGROUND	137:146	BACKGROUND AND AIMS Brown algae are photosynthetic multicellular marine organisms evolutionarily distant from land plants, with a distinctive cell wall.
24875633	1	65	theme	marine	202:207	arg1	organisms					209:217	photosynthetic multicellular marine organisms	173:217	photosynthetic multicellular marine organisms evolutionarily distant from land plants	173:257	BACKGROUND AND AIMS Brown algae are photosynthetic multicellular marine organisms evolutionarily distant from land plants, with a distinctive cell wall.
24875633	11	66	theme	1→3	1385:1387	arg1	structure					1399:1407	a regular α-(1→3) backbone structure	1372:1407	a regular α-(1→3) backbone structure	1372:1407	The sulfated fucans from H. elongata were shown to have a regular α-(1→3) backbone structure, while an alternating α-(1→3), (1→4) structure has been described in some brown algae from the order Fucales.
24875633	12	67	theme	wall	1578:1581	arg1	architecture					1583:1594	the cell wall architecture	1569:1594	the cell wall architecture	1569:1594	CONCLUSIONS The data provide a global snapshot of the cell wall architecture in brown algae, and contribute to the understanding of the structure-function relationships of the main cell wall components.
24875633	11	68	theme	brown	1483:1487	arg1	algae					1489:1493	some brown algae	1478:1493	some brown algae from the order Fucales	1478:1516	The sulfated fucans from H. elongata were shown to have a regular α-(1→3) backbone structure, while an alternating α-(1→3), (1→4) structure has been described in some brown algae from the order Fucales.
24875633	14	69	theme	ECM	1931:1933	arg1	components					1935:1944	ECM components	1931:1944	ECM components	1931:1944	The emergence and evolution of ECM components is further discussed in relation to the evolution of multicellularity in brown algae.
24875633	12	70	theme	main	1695:1698	arg1	components					1710:1719	the main cell wall components	1691:1719	the main cell wall components	1691:1719	CONCLUSIONS The data provide a global snapshot of the cell wall architecture in brown algae, and contribute to the understanding of the structure-function relationships of the main cell wall components.
24875633	5	71	theme	cell	772:775	arg1	material					782:789	cell wall material	772:789	cell wall material from various brown algae of the order Fucales	772:835	METHODS Exhaustive sequential extractions and composition analyses of cell wall material from various brown algae of the order Fucales were performed.
24875633	12	72	theme	wall	1705:1708	arg1	components					1710:1719	the main cell wall components	1691:1719	the main cell wall components	1691:1719	CONCLUSIONS The data provide a global snapshot of the cell wall architecture in brown algae, and contribute to the understanding of the structure-function relationships of the main cell wall components.
24875633	11	73	theme	order	1504:1508	arg1	Fucales					1510:1516	the order Fucales	1500:1516	the order Fucales	1500:1516	The sulfated fucans from H. elongata were shown to have a regular α-(1→3) backbone structure, while an alternating α-(1→3), (1→4) structure has been described in some brown algae from the order Fucales.
24875633	5	74	theme	material	782:789	arg1	analyses					760:767	composition analyses	748:767	composition analyses	748:767	METHODS Exhaustive sequential extractions and composition analyses of cell wall material from various brown algae of the order Fucales were performed.
24875633	5	74	theme	material	782:789	arg1	METHODS					702:708	METHODS	702:708	METHODS	702:708	METHODS Exhaustive sequential extractions and composition analyses of cell wall material from various brown algae of the order Fucales were performed.
24875633	12	75	from	snapshot	1557:1564	arg1	algae					1605:1609	brown algae	1599:1609	brown algae	1599:1609	CONCLUSIONS The data provide a global snapshot of the cell wall architecture in brown algae, and contribute to the understanding of the structure-function relationships of the main cell wall components.
24875633	7	76	theme	polymer	1019:1025	arg1	interlinks					1027:1036	polymer interlinks	1019:1036	polymer interlinks	1019:1036	This approach was complemented by systematic chromatographic analysis to study polymer interlinks further.
24875633	12	77	theme	structure-function	1655:1672	arg1	relationships					1674:1686	the structure-function relationships	1651:1686	the structure-function relationships of the main cell wall components	1651:1719	CONCLUSIONS The data provide a global snapshot of the cell wall architecture in brown algae, and contribute to the understanding of the structure-function relationships of the main cell wall components.
24875633	0	78	theme	extracellular	100:112	arg1	matrix					114:119	the extracellular matrix	96:119	the extracellular matrix of brown algae	96:134	Chemical and enzymatic fractionation of cell walls from Fucales: insights into the structure of the extracellular matrix of brown algae.
24875633	8	79	theme	sulfated	1090:1097	arg1	fucan					1099:1103	the sulfated fucan	1086:1103	the sulfated fucan extracted from Himanthalia elongata	1086:1139	An additional structural assessment of the sulfated fucan extracted from Himanthalia elongata was made.
24875633	14	80	from	evolution	1986:1994	arg1	algae					2025:2029	brown algae	2019:2029	brown algae	2019:2029	The emergence and evolution of ECM components is further discussed in relation to the evolution of multicellularity in brown algae.
24875633	11	81	theme	1→4	1441:1443	arg1	α-					1431:1432	an alternating α-	1416:1432	an alternating α-(1→3)	1416:1437	The sulfated fucans from H. elongata were shown to have a regular α-(1→3) backbone structure, while an alternating α-(1→3), (1→4) structure has been described in some brown algae from the order Fucales.
24875633	11	81	theme	1→4	1441:1443	arg1	structure					1446:1454	(1→4) structure	1440:1454	(1→4) structure	1440:1454	The sulfated fucans from H. elongata were shown to have a regular α-(1→3) backbone structure, while an alternating α-(1→3), (1→4) structure has been described in some brown algae from the order Fucales.
24875633	7	82	theme	chromatographic	985:999	arg1	analysis					1001:1008	systematic chromatographic analysis	974:1008	systematic chromatographic analysis to study polymer interlinks further	974:1044	This approach was complemented by systematic chromatographic analysis to study polymer interlinks further.
24875633	2	83	dep	animals	349:355	arg1	polysaccharides					385:399	fucose-containing sulfated polysaccharides	358:399	fucose-containing sulfated polysaccharides	358:399	They feature carbohydrates shared with plants (cellulose), animals (fucose-containing sulfated polysaccharides, FCSPs) or bacteria (alginates).
24875633	2	83	dep	animals	349:355	arg1	FCSPs					402:406	FCSPs	402:406	FCSPs	402:406	They feature carbohydrates shared with plants (cellulose), animals (fucose-containing sulfated polysaccharides, FCSPs) or bacteria (alginates).
24875633	11	84	contain	have	1367:1370	arg2	structure					1399:1407	a regular α-(1→3) backbone structure	1372:1407	a regular α-(1→3) backbone structure	1372:1407	The sulfated fucans from H. elongata were shown to have a regular α-(1→3) backbone structure, while an alternating α-(1→3), (1→4) structure has been described in some brown algae from the order Fucales.
24875633	11	84	contain	have	1367:1370	arg1	fucans					1329:1334	The sulfated fucans	1316:1334	The sulfated fucans from H. elongata	1316:1351	The sulfated fucans from H. elongata were shown to have a regular α-(1→3) backbone structure, while an alternating α-(1→3), (1→4) structure has been described in some brown algae from the order Fucales.
24875633	1	85	from	plants	252:257	arg1	distant					234:240	distant	234:240	distant	234:240	BACKGROUND AND AIMS Brown algae are photosynthetic multicellular marine organisms evolutionarily distant from land plants, with a distinctive cell wall.
24875633	4	86	theme	Recent	544:549	arg1	analysis					561:568	Recent molecular analysis	544:568	Recent molecular analysis of the corresponding biosynthetic routes	544:609	Recent molecular analysis of the corresponding biosynthetic routes points toward a complex evolutionary history that shaped the ECM structure in brown algae.
24875633	9	87	theme	KEY	1151:1153	arg1	RESULTS					1155:1161	KEY RESULTS	1151:1161	KEY RESULTS The data indicate that FCSPs are tightly associated with proteins and cellulose within the walls.	1151:1259	KEY RESULTS The data indicate that FCSPs are tightly associated with proteins and cellulose within the walls.
24875633	2	88	theme	fucose-containing	358:374	arg1	polysaccharides					385:399	fucose-containing sulfated polysaccharides	358:399	fucose-containing sulfated polysaccharides	358:399	They feature carbohydrates shared with plants (cellulose), animals (fucose-containing sulfated polysaccharides, FCSPs) or bacteria (alginates).
24875633	2	88	theme	fucose-containing	358:374	arg1	FCSPs					402:406	FCSPs	402:406	FCSPs	402:406	They feature carbohydrates shared with plants (cellulose), animals (fucose-containing sulfated polysaccharides, FCSPs) or bacteria (alginates).
24875633	5	89	theme	Fucales	829:835	arg1	algae					810:814	various brown algae	796:814	various brown algae of the order Fucales	796:835	METHODS Exhaustive sequential extractions and composition analyses of cell wall material from various brown algae of the order Fucales were performed.
24875633	1	90	theme	cell	279:282	arg1	wall					284:287	a distinctive cell wall	265:287	a distinctive cell wall	265:287	BACKGROUND AND AIMS Brown algae are photosynthetic multicellular marine organisms evolutionarily distant from land plants, with a distinctive cell wall.
24875633	5	91	theme	various	796:802	arg1	algae					810:814	various brown algae	796:814	various brown algae of the order Fucales	796:835	METHODS Exhaustive sequential extractions and composition analyses of cell wall material from various brown algae of the order Fucales were performed.
24875633	13	92	theme	Enzymatic	1722:1730	arg1	cross-linking					1732:1744	Enzymatic cross-linking	1722:1744	Enzymatic cross-linking of alginates by phenols	1722:1768	Enzymatic cross-linking of alginates by phenols may regulate the strengthening of the wall, and sulfated polysaccharides may play a key role in the adaptation to osmotic stress.
24875633	12	93	dep	CONCLUSIONS	1519:1529	arg1	contribute					1616:1625	contribute	1616:1625	contribute to the understanding of the structure-function relationships of the main cell wall components	1616:1719	CONCLUSIONS The data provide a global snapshot of the cell wall architecture in brown algae, and contribute to the understanding of the structure-function relationships of the main cell wall components.
24875633	12	93	dep	CONCLUSIONS	1519:1529	arg1	provide					1540:1546	provide	1540:1546	provide a global snapshot of the cell wall architecture in brown algae	1540:1609	CONCLUSIONS The data provide a global snapshot of the cell wall architecture in brown algae, and contribute to the understanding of the structure-function relationships of the main cell wall components.
24875633	6	94	used	used	891:894	arg2	degradations					873:884	Dedicated enzymatic degradations	853:884	Dedicated enzymatic degradations	853:884	Dedicated enzymatic degradations were used to release and identify cell wall partners.
24875633	13	95	theme	wall	1808:1811	arg1	strengthening					1787:1799	the strengthening	1783:1799	the strengthening of the wall	1783:1811	Enzymatic cross-linking of alginates by phenols may regulate the strengthening of the wall, and sulfated polysaccharides may play a key role in the adaptation to osmotic stress.
24875633	11	96	theme	sulfated	1320:1327	arg1	fucans					1329:1334	The sulfated fucans	1316:1334	The sulfated fucans from H. elongata	1316:1351	The sulfated fucans from H. elongata were shown to have a regular α-(1→3) backbone structure, while an alternating α-(1→3), (1→4) structure has been described in some brown algae from the order Fucales.
24875633	8	97	theme	fucan	1099:1103	arg1	assessment					1072:1081	An additional structural assessment	1047:1081	An additional structural assessment of the sulfated fucan extracted from Himanthalia elongata	1047:1139	An additional structural assessment of the sulfated fucan extracted from Himanthalia elongata was made.
24875633	3	98	theme	three-dimensional	476:492	arg1	matrix					508:513	a three-dimensional extracellular matrix	474:513	a three-dimensional extracellular matrix (ECM)	474:519	How these components are organized into a three-dimensional extracellular matrix (ECM) still remains unclear.
24875633	3	98	theme	three-dimensional	476:492	arg1	ECM					516:518	ECM	516:518	ECM	516:518	How these components are organized into a three-dimensional extracellular matrix (ECM) still remains unclear.
24875633	6	99	theme	Dedicated	853:861	arg1	degradations					873:884	Dedicated enzymatic degradations	853:884	Dedicated enzymatic degradations	853:884	Dedicated enzymatic degradations were used to release and identify cell wall partners.
24875633	0	100	dep	fractionation	23:35	arg1	insights					65:72	insights	65:72	Chemical and enzymatic fractionation of cell walls from Fucales: insights into the structure of the extracellular matrix of brown algae.	0:135	Chemical and enzymatic fractionation of cell walls from Fucales: insights into the structure of the extracellular matrix of brown algae.
24875633	14	101	theme	brown	2019:2023	arg1	algae					2025:2029	brown algae	2019:2029	brown algae	2019:2029	The emergence and evolution of ECM components is further discussed in relation to the evolution of multicellularity in brown algae.
24875633	1	102	theme	distant	234:240	arg1	AIMS					152:155	AIMS	152:155	AIMS	152:155	BACKGROUND AND AIMS Brown algae are photosynthetic multicellular marine organisms evolutionarily distant from land plants, with a distinctive cell wall.
24875633	1	102	theme	distant	234:240	arg1	BACKGROUND					137:146	BACKGROUND	137:146	BACKGROUND	137:146	BACKGROUND AND AIMS Brown algae are photosynthetic multicellular marine organisms evolutionarily distant from land plants, with a distinctive cell wall.
24875633	1	102	theme	distant	234:240	arg1	organisms					209:217	photosynthetic multicellular marine organisms	173:217	photosynthetic multicellular marine organisms evolutionarily distant from land plants	173:257	BACKGROUND AND AIMS Brown algae are photosynthetic multicellular marine organisms evolutionarily distant from land plants, with a distinctive cell wall.
24875633	11	103	theme	alternating	1419:1429	arg1	1→3					1434:1436	1→3	1434:1436	1→3	1434:1436	The sulfated fucans from H. elongata were shown to have a regular α-(1→3) backbone structure, while an alternating α-(1→3), (1→4) structure has been described in some brown algae from the order Fucales.
24875633	11	103	theme	alternating	1419:1429	arg1	structure					1446:1454	(1→4) structure	1440:1454	(1→4) structure	1440:1454	The sulfated fucans from H. elongata were shown to have a regular α-(1→3) backbone structure, while an alternating α-(1→3), (1→4) structure has been described in some brown algae from the order Fucales.
24875633	11	103	theme	alternating	1419:1429	arg1	α-					1431:1432	an alternating α-	1416:1432	an alternating α-(1→3)	1416:1437	The sulfated fucans from H. elongata were shown to have a regular α-(1→3) backbone structure, while an alternating α-(1→3), (1→4) structure has been described in some brown algae from the order Fucales.
24875633	1	104	theme	land	247:250	arg1	plants					252:257	land plants	247:257	land plants	247:257	BACKGROUND AND AIMS Brown algae are photosynthetic multicellular marine organisms evolutionarily distant from land plants, with a distinctive cell wall.
27664924	6	0	theme	size	876:879	arg1	measurements					881:892	the pore size measurements	867:892	the pore size measurements	867:892	In spite of such decrease, the pore size measurements were in the optimal range for bone regeneration.
27664924	6	1	theme	optimal	906:912	arg1	range					914:918	the optimal range	902:918	the optimal range for bone regeneration	902:940	In spite of such decrease, the pore size measurements were in the optimal range for bone regeneration.
27664924	7	2	theme	mineralization	965:978	arg1	ratio					1034:1038	The bone-like apatite mineralization, compressive strength, carboxyl content, and swelling ratio	943:1038	The bone-like apatite mineralization, compressive strength, carboxyl content, and swelling ratio of the composites	943:1056	The bone-like apatite mineralization, compressive strength, carboxyl content, and swelling ratio of the composites increased with additional starch.
27664924	1	3	theme	bone	197:200	arg1	regeneration					202:213	bone regeneration	197:213	bone regeneration	197:213	This study offers new bioactive composite scaffolds from carboxylated starch-chitosan for bone regeneration.
27664924	8	4	theme	ALP	1184:1186	arg1	activity					1188:1195	ALP activity	1184:1195	ALP activity	1184:1195	Cell culture experiments demonstrated that higher starch content can enhance proliferation, ALP activity, and mineralization of osteoblast-like cells (MG63).
27664924	7	5	theme	strength	993:1000	arg1	ratio					1034:1038	The bone-like apatite mineralization, compressive strength, carboxyl content, and swelling ratio	943:1038	The bone-like apatite mineralization, compressive strength, carboxyl content, and swelling ratio of the composites	943:1056	The bone-like apatite mineralization, compressive strength, carboxyl content, and swelling ratio of the composites increased with additional starch.
27664924	4	6	theme	pore	628:631	arg1	size					633:636	pore size	628:636	pore size	628:636	The results indicated that chitosan scaffolds showed the highest pore size and porosity, while no apatite deposition was observed even after 14days of soaking in simulated body fluid.
27664924	5	7	theme	composite	751:759	arg1	samples					761:767	composite samples	751:767	composite samples	751:767	For composite samples, the pore size and porosity decreased as the starch content increased.
27664924	7	8	theme	bone-like	947:955	arg1	mineralization					965:978	bone-like apatite mineralization	947:978	bone-like apatite mineralization	947:978	The bone-like apatite mineralization, compressive strength, carboxyl content, and swelling ratio of the composites increased with additional starch.
27664924	4	9	theme	highest	620:626	arg1	size					633:636	pore size	628:636	pore size	628:636	The results indicated that chitosan scaffolds showed the highest pore size and porosity, while no apatite deposition was observed even after 14days of soaking in simulated body fluid.
27664924	3	10	theme	Various	363:369	arg1	techniques					388:397	Various characterization techniques	363:397	Various characterization techniques	363:397	Various characterization techniques were used to analyze the structure, morphology, compressive strength, and apatite mineralization of the composites, which were compared to pure chitosan scaffolds.
27664924	4	11	theme	body	735:738	arg1	fluid					740:744	simulated body fluid	725:744	simulated body fluid	725:744	The results indicated that chitosan scaffolds showed the highest pore size and porosity, while no apatite deposition was observed even after 14days of soaking in simulated body fluid.
27664924	1	12	from	starch-chitosan	177:191	arg1	scaffolds					149:157	new bioactive composite scaffolds	125:157	new bioactive composite scaffolds from carboxylated starch-chitosan for bone regeneration	125:213	This study offers new bioactive composite scaffolds from carboxylated starch-chitosan for bone regeneration.
27664924	2	13	theme	citric	302:307	arg1	acid					309:312	citric acid	302:312	citric acid	302:312	In order to introduce COOH groups into the scaffolds, chitosan was first dissolved in citric acid and then mixed with different amounts of starch.
27664924	3	14	theme	characterization	371:386	arg1	techniques					388:397	Various characterization techniques	363:397	Various characterization techniques	363:397	Various characterization techniques were used to analyze the structure, morphology, compressive strength, and apatite mineralization of the composites, which were compared to pure chitosan scaffolds.
27664924	5	15	theme	starch	814:819	arg1	content					821:827	the starch content	810:827	the starch content	810:827	For composite samples, the pore size and porosity decreased as the starch content increased.
27664924	4	16	theme	chitosan	590:597	arg1	scaffolds					599:607	chitosan scaffolds	590:607	chitosan scaffolds	590:607	The results indicated that chitosan scaffolds showed the highest pore size and porosity, while no apatite deposition was observed even after 14days of soaking in simulated body fluid.
27664924	2	17	theme	different	334:342	arg1	starch					355:360	starch	355:360	starch	355:360	In order to introduce COOH groups into the scaffolds, chitosan was first dissolved in citric acid and then mixed with different amounts of starch.
27664924	2	17	theme	different	334:342	arg1	amounts					344:350	different amounts	334:350	different amounts of starch	334:360	In order to introduce COOH groups into the scaffolds, chitosan was first dissolved in citric acid and then mixed with different amounts of starch.
27664924	8	18	theme	Cell	1092:1095	arg1	experiments					1105:1115	Cell culture experiments	1092:1115	Cell culture experiments	1092:1115	Cell culture experiments demonstrated that higher starch content can enhance proliferation, ALP activity, and mineralization of osteoblast-like cells (MG63).
27664924	3	19	theme	compressive	447:457	arg1	strength					459:466	compressive strength	447:466	compressive strength	447:466	Various characterization techniques were used to analyze the structure, morphology, compressive strength, and apatite mineralization of the composites, which were compared to pure chitosan scaffolds.
27664924	4	20	theme	soaking	714:720	arg1	14days					704:709	14days	704:709	14days of soaking	704:720	The results indicated that chitosan scaffolds showed the highest pore size and porosity, while no apatite deposition was observed even after 14days of soaking in simulated body fluid.
27664924	8	21	theme	osteoblast-like	1220:1234	arg1	MG63					1243:1246	MG63	1243:1246	MG63	1243:1246	Cell culture experiments demonstrated that higher starch content can enhance proliferation, ALP activity, and mineralization of osteoblast-like cells (MG63).
27664924	8	21	theme	osteoblast-like	1220:1234	arg1	cells					1236:1240	osteoblast-like cells	1220:1240	osteoblast-like cells (MG63)	1220:1247	Cell culture experiments demonstrated that higher starch content can enhance proliferation, ALP activity, and mineralization of osteoblast-like cells (MG63).
27664924	7	22	theme	additional	1073:1082	arg1	starch					1084:1089	additional starch	1073:1089	additional starch	1073:1089	The bone-like apatite mineralization, compressive strength, carboxyl content, and swelling ratio of the composites increased with additional starch.
27664924	7	23	theme	apatite	957:963	arg1	mineralization					965:978	bone-like apatite mineralization	947:978	bone-like apatite mineralization	947:978	The bone-like apatite mineralization, compressive strength, carboxyl content, and swelling ratio of the composites increased with additional starch.
27664924	3	24	used	used	404:407	arg2	techniques					388:397	Various characterization techniques	363:397	Various characterization techniques	363:397	Various characterization techniques were used to analyze the structure, morphology, compressive strength, and apatite mineralization of the composites, which were compared to pure chitosan scaffolds.
27664924	6	25	theme	pore	871:874	arg1	measurements					881:892	the pore size measurements	867:892	the pore size measurements	867:892	In spite of such decrease, the pore size measurements were in the optimal range for bone regeneration.
27664924	1	26	theme	new	125:127	arg1	scaffolds					149:157	new bioactive composite scaffolds	125:157	new bioactive composite scaffolds from carboxylated starch-chitosan for bone regeneration	125:213	This study offers new bioactive composite scaffolds from carboxylated starch-chitosan for bone regeneration.
27664924	1	27	theme	carboxylated	164:175	arg1	starch-chitosan					177:191	carboxylated starch-chitosan	164:191	carboxylated starch-chitosan for bone regeneration	164:213	This study offers new bioactive composite scaffolds from carboxylated starch-chitosan for bone regeneration.
27664924	0	28	theme	starch-chitosan	49:63	arg1	scaffold					75:82	starch-chitosan bioactive scaffold	49:82	starch-chitosan bioactive scaffold for bone regeneration	49:104	Fabrication and characterization of carboxylated starch-chitosan bioactive scaffold for bone regeneration.
27664924	2	29	theme	starch	355:360	arg1	starch					355:360	starch	355:360	starch	355:360	In order to introduce COOH groups into the scaffolds, chitosan was first dissolved in citric acid and then mixed with different amounts of starch.
27664924	2	29	theme	starch	355:360	arg1	amounts					344:350	different amounts	334:350	different amounts of starch	334:360	In order to introduce COOH groups into the scaffolds, chitosan was first dissolved in citric acid and then mixed with different amounts of starch.
27664924	4	30	located	observed	684:691	arg2	deposition					669:678	no apatite deposition	658:678	no apatite deposition	658:678	The results indicated that chitosan scaffolds showed the highest pore size and porosity, while no apatite deposition was observed even after 14days of soaking in simulated body fluid.
27664924	4	30	located	observed	684:691	arg1	fluid					740:744	simulated body fluid	725:744	simulated body fluid	725:744	The results indicated that chitosan scaffolds showed the highest pore size and porosity, while no apatite deposition was observed even after 14days of soaking in simulated body fluid.
27664924	3	31	theme	apatite	473:479	arg1	mineralization					481:494	apatite mineralization	473:494	apatite mineralization	473:494	Various characterization techniques were used to analyze the structure, morphology, compressive strength, and apatite mineralization of the composites, which were compared to pure chitosan scaffolds.
27664924	8	32	theme	cells	1236:1240	arg1	proliferation					1169:1181	proliferation	1169:1181	proliferation	1169:1181	Cell culture experiments demonstrated that higher starch content can enhance proliferation, ALP activity, and mineralization of osteoblast-like cells (MG63).
27664924	8	32	theme	cells	1236:1240	arg1	activity					1188:1195	ALP activity	1184:1195	ALP activity	1184:1195	Cell culture experiments demonstrated that higher starch content can enhance proliferation, ALP activity, and mineralization of osteoblast-like cells (MG63).
27664924	8	32	theme	cells	1236:1240	arg1	mineralization					1202:1215	mineralization	1202:1215	mineralization	1202:1215	Cell culture experiments demonstrated that higher starch content can enhance proliferation, ALP activity, and mineralization of osteoblast-like cells (MG63).
27664924	8	33	theme	culture	1097:1103	arg1	experiments					1105:1115	Cell culture experiments	1092:1115	Cell culture experiments	1092:1115	Cell culture experiments demonstrated that higher starch content can enhance proliferation, ALP activity, and mineralization of osteoblast-like cells (MG63).
27664924	4	34	theme	apatite	661:667	arg1	deposition					669:678	no apatite deposition	658:678	no apatite deposition	658:678	The results indicated that chitosan scaffolds showed the highest pore size and porosity, while no apatite deposition was observed even after 14days of soaking in simulated body fluid.
27664924	7	35	theme	swelling	1025:1032	arg1	ratio					1034:1038	The bone-like apatite mineralization, compressive strength, carboxyl content, and swelling ratio	943:1038	The bone-like apatite mineralization, compressive strength, carboxyl content, and swelling ratio of the composites	943:1056	The bone-like apatite mineralization, compressive strength, carboxyl content, and swelling ratio of the composites increased with additional starch.
27664924	1	36	theme	bioactive	129:137	arg1	scaffolds					149:157	new bioactive composite scaffolds	125:157	new bioactive composite scaffolds from carboxylated starch-chitosan for bone regeneration	125:213	This study offers new bioactive composite scaffolds from carboxylated starch-chitosan for bone regeneration.
27664924	0	37	theme	bioactive	65:73	arg1	scaffold					75:82	starch-chitosan bioactive scaffold	49:82	starch-chitosan bioactive scaffold for bone regeneration	49:104	Fabrication and characterization of carboxylated starch-chitosan bioactive scaffold for bone regeneration.
27664924	7	38	theme	composites	1047:1056	arg1	ratio					1034:1038	The bone-like apatite mineralization, compressive strength, carboxyl content, and swelling ratio	943:1038	The bone-like apatite mineralization, compressive strength, carboxyl content, and swelling ratio of the composites	943:1056	The bone-like apatite mineralization, compressive strength, carboxyl content, and swelling ratio of the composites increased with additional starch.
27664924	6	39	theme	such	852:855	arg1	decrease					857:864	such decrease	852:864	such decrease	852:864	In spite of such decrease, the pore size measurements were in the optimal range for bone regeneration.
27664924	7	40	theme	content	1012:1018	arg1	ratio					1034:1038	The bone-like apatite mineralization, compressive strength, carboxyl content, and swelling ratio	943:1038	The bone-like apatite mineralization, compressive strength, carboxyl content, and swelling ratio of the composites	943:1056	The bone-like apatite mineralization, compressive strength, carboxyl content, and swelling ratio of the composites increased with additional starch.
27664924	1	41	theme	composite	139:147	arg1	scaffolds					149:157	new bioactive composite scaffolds	125:157	new bioactive composite scaffolds from carboxylated starch-chitosan for bone regeneration	125:213	This study offers new bioactive composite scaffolds from carboxylated starch-chitosan for bone regeneration.
27664924	6	42	theme	bone	924:927	arg1	regeneration					929:940	bone regeneration	924:940	bone regeneration	924:940	In spite of such decrease, the pore size measurements were in the optimal range for bone regeneration.
27664924	8	43	theme	starch	1142:1147	arg1	content					1149:1155	higher starch content	1135:1155	higher starch content	1135:1155	Cell culture experiments demonstrated that higher starch content can enhance proliferation, ALP activity, and mineralization of osteoblast-like cells (MG63).
27664924	2	44	theme	COOH	238:241	arg1	groups					243:248	COOH groups	238:248	COOH groups	238:248	In order to introduce COOH groups into the scaffolds, chitosan was first dissolved in citric acid and then mixed with different amounts of starch.
27664924	3	45	theme	composites	503:512	arg1	mineralization					481:494	apatite mineralization	473:494	apatite mineralization	473:494	Various characterization techniques were used to analyze the structure, morphology, compressive strength, and apatite mineralization of the composites, which were compared to pure chitosan scaffolds.
27664924	3	45	theme	composites	503:512	arg1	morphology					435:444	morphology	435:444	morphology	435:444	Various characterization techniques were used to analyze the structure, morphology, compressive strength, and apatite mineralization of the composites, which were compared to pure chitosan scaffolds.
27664924	3	45	theme	composites	503:512	arg1	strength					459:466	compressive strength	447:466	compressive strength	447:466	Various characterization techniques were used to analyze the structure, morphology, compressive strength, and apatite mineralization of the composites, which were compared to pure chitosan scaffolds.
27664924	3	45	theme	composites	503:512	arg1	structure					424:432	structure	424:432	structure	424:432	Various characterization techniques were used to analyze the structure, morphology, compressive strength, and apatite mineralization of the composites, which were compared to pure chitosan scaffolds.
27664924	8	46	theme	higher	1135:1140	arg1	content					1149:1155	higher starch content	1135:1155	higher starch content	1135:1155	Cell culture experiments demonstrated that higher starch content can enhance proliferation, ALP activity, and mineralization of osteoblast-like cells (MG63).
27664924	0	47	theme	bone	88:91	arg1	regeneration					93:104	bone regeneration	88:104	bone regeneration	88:104	Fabrication and characterization of carboxylated starch-chitosan bioactive scaffold for bone regeneration.
27664924	7	48	theme	carboxyl	1003:1010	arg1	content					1012:1018	carboxyl content	1003:1018	carboxyl content	1003:1018	The bone-like apatite mineralization, compressive strength, carboxyl content, and swelling ratio of the composites increased with additional starch.
27664924	3	49	theme	pure	538:541	arg1	scaffolds					552:560	pure chitosan scaffolds	538:560	pure chitosan scaffolds	538:560	Various characterization techniques were used to analyze the structure, morphology, compressive strength, and apatite mineralization of the composites, which were compared to pure chitosan scaffolds.
27664924	5	50	theme	pore	774:777	arg1	size					779:782	the pore size	770:782	the pore size	770:782	For composite samples, the pore size and porosity decreased as the starch content increased.
27664924	7	51	theme	compressive	981:991	arg1	strength					993:1000	compressive strength	981:1000	compressive strength	981:1000	The bone-like apatite mineralization, compressive strength, carboxyl content, and swelling ratio of the composites increased with additional starch.
27664924	3	52	theme	chitosan	543:550	arg1	scaffolds					552:560	pure chitosan scaffolds	538:560	pure chitosan scaffolds	538:560	Various characterization techniques were used to analyze the structure, morphology, compressive strength, and apatite mineralization of the composites, which were compared to pure chitosan scaffolds.
27664924	4	53	theme	simulated	725:733	arg1	fluid					740:744	simulated body fluid	725:744	simulated body fluid	725:744	The results indicated that chitosan scaffolds showed the highest pore size and porosity, while no apatite deposition was observed even after 14days of soaking in simulated body fluid.
24906775	5	0	theme	specific	722:729	arg1	strength					731:738	high specific strength	717:738	high specific strength	717:738	Due to its light weight and the presence of high cellulose content (77.17%) with very little wax (0.14%) provide high specific strength and good bonding properties.
24906775	3	1	theme	root	359:362	arg1	fiber					364:368	Cissus quadrangularis root fiber	337:368	Cissus quadrangularis root fiber	337:368	Comprehensive characterization of Cissus quadrangularis root fiber such as anatomical study, chemical analysis, physical analysis, FTIR, XRD, SEM analysis and thermo gravimetric analysis are done.
24906775	6	2	theme	high	886:889	arg1	modulus					891:897	its high modulus	882:897	its high modulus	882:897	The flaky honeycomb outer surface and low microfibril angle revealed through electron microscopy contributes for its high modulus.
24906775	5	3	theme	high	648:651	arg1	%					677:677	77.17%	672:677	77.17%	672:677	Due to its light weight and the presence of high cellulose content (77.17%) with very little wax (0.14%) provide high specific strength and good bonding properties.
24906775	5	3	theme	high	648:651	arg1	content					663:669	high cellulose content	648:669	high cellulose content (77.17%)	648:678	Due to its light weight and the presence of high cellulose content (77.17%) with very little wax (0.14%) provide high specific strength and good bonding properties.
24906775	6	4	theme	honeycomb	779:787	arg1	surface					795:801	The flaky honeycomb outer surface	769:801	The flaky honeycomb outer surface	769:801	The flaky honeycomb outer surface and low microfibril angle revealed through electron microscopy contributes for its high modulus.
24906775	1	5	theme	high	168:171	arg1	strength					182:189	its high specific strength	164:189	its high specific strength	164:189	Fiber reinforced polymer composites are replacing many metallic structures due to its high specific strength and modulus.
24906775	0	6	from	Characterization	0:15	arg1	root					76:79	Cissus quadrangularis root	54:79	Cissus quadrangularis root	54:79	Characterization of new natural cellulosic fiber from Cissus quadrangularis root.
24906775	5	7	theme	cellulose	653:661	arg1	%					677:677	77.17%	672:677	77.17%	672:677	Due to its light weight and the presence of high cellulose content (77.17%) with very little wax (0.14%) provide high specific strength and good bonding properties.
24906775	5	7	theme	cellulose	653:661	arg1	content					663:669	high cellulose content	648:669	high cellulose content (77.17%)	648:678	Due to its light weight and the presence of high cellulose content (77.17%) with very little wax (0.14%) provide high specific strength and good bonding properties.
24906775	6	8	theme	flaky	773:777	arg1	surface					795:801	The flaky honeycomb outer surface	769:801	The flaky honeycomb outer surface	769:801	The flaky honeycomb outer surface and low microfibril angle revealed through electron microscopy contributes for its high modulus.
24906775	5	9	theme	content	663:669	arg1	weight					621:626	its light weight	611:626	its light weight	611:626	Due to its light weight and the presence of high cellulose content (77.17%) with very little wax (0.14%) provide high specific strength and good bonding properties.
24906775	5	9	theme	content	663:669	arg1	presence					636:643	the presence	632:643	the presence of high cellulose content (77.17%) with very little wax (0.14%)	632:707	Due to its light weight and the presence of high cellulose content (77.17%) with very little wax (0.14%) provide high specific strength and good bonding properties.
24906775	5	10	theme	light	615:619	arg1	weight					621:626	its light weight	611:626	its light weight	611:626	Due to its light weight and the presence of high cellulose content (77.17%) with very little wax (0.14%) provide high specific strength and good bonding properties.
24906775	1	11	theme	specific	173:180	arg1	strength					182:189	its high specific strength	164:189	its high specific strength	164:189	Fiber reinforced polymer composites are replacing many metallic structures due to its high specific strength and modulus.
24906775	3	12	theme	anatomical	378:387	arg1	study					389:393	anatomical study	378:393	anatomical study	378:393	Comprehensive characterization of Cissus quadrangularis root fiber such as anatomical study, chemical analysis, physical analysis, FTIR, XRD, SEM analysis and thermo gravimetric analysis are done.
24906775	6	13	theme	microfibril	811:821	arg1	angle					823:827	low microfibril angle	807:827	low microfibril angle	807:827	The flaky honeycomb outer surface and low microfibril angle revealed through electron microscopy contributes for its high modulus.
24906775	1	14	theme	Fiber	82:86	arg1	structures					146:155	replacing many metallic structures	122:155	replacing many metallic structures due to its high specific strength and modulus	122:201	Fiber reinforced polymer composites are replacing many metallic structures due to its high specific strength and modulus.
24906775	1	14	theme	Fiber	82:86	arg1	composites					107:116	Fiber reinforced polymer composites	82:116	Fiber reinforced polymer composites	82:116	Fiber reinforced polymer composites are replacing many metallic structures due to its high specific strength and modulus.
24906775	6	15	theme	electron	846:853	arg1	microscopy					855:864	electron microscopy	846:864	electron microscopy	846:864	The flaky honeycomb outer surface and low microfibril angle revealed through electron microscopy contributes for its high modulus.
24906775	7	16	theme	polymerization	1018:1031	arg1	process					1033:1039	the polymerization process	1014:1039	the polymerization process temperature	1014:1051	The thermo gravimetric analysis indicates better thermal stability of the fiber up to 230°C, which is well within the polymerization process temperature.
24906775	1	17	theme	reinforced	88:97	arg1	structures					146:155	replacing many metallic structures	122:155	replacing many metallic structures due to its high specific strength and modulus	122:201	Fiber reinforced polymer composites are replacing many metallic structures due to its high specific strength and modulus.
24906775	1	17	theme	reinforced	88:97	arg1	composites					107:116	Fiber reinforced polymer composites	82:116	Fiber reinforced polymer composites	82:116	Fiber reinforced polymer composites are replacing many metallic structures due to its high specific strength and modulus.
24906775	3	18	theme	physical	415:422	arg1	analysis					424:431	physical analysis	415:431	physical analysis	415:431	Comprehensive characterization of Cissus quadrangularis root fiber such as anatomical study, chemical analysis, physical analysis, FTIR, XRD, SEM analysis and thermo gravimetric analysis are done.
24906775	1	19	theme	polymer	99:105	arg1	structures					146:155	replacing many metallic structures	122:155	replacing many metallic structures due to its high specific strength and modulus	122:201	Fiber reinforced polymer composites are replacing many metallic structures due to its high specific strength and modulus.
24906775	1	19	theme	polymer	99:105	arg1	composites					107:116	Fiber reinforced polymer composites	82:116	Fiber reinforced polymer composites	82:116	Fiber reinforced polymer composites are replacing many metallic structures due to its high specific strength and modulus.
24906775	0	20	theme	natural	24:30	arg1	fiber					43:47	new natural cellulosic fiber	20:47	new natural cellulosic fiber from Cissus quadrangularis root	20:79	Characterization of new natural cellulosic fiber from Cissus quadrangularis root.
24906775	4	21	theme	fiber	556:560	arg1	industries					562:571	fiber industries	556:571	fiber industries	556:571	The results are very encouraging for its application in fiber industries, composite manufacturing, etc.
24906775	7	22	theme	thermo	904:909	arg1	analysis					923:930	The thermo gravimetric analysis	900:930	The thermo gravimetric analysis	900:930	The thermo gravimetric analysis indicates better thermal stability of the fiber up to 230°C, which is well within the polymerization process temperature.
24906775	5	23	theme	bonding	749:755	arg1	properties					757:766	good bonding properties	744:766	good bonding properties	744:766	Due to its light weight and the presence of high cellulose content (77.17%) with very little wax (0.14%) provide high specific strength and good bonding properties.
24906775	7	24	theme	thermal	949:955	arg1	stability					957:965	better thermal stability	942:965	better thermal stability of the fiber up to 230°C, which is well within the polymerization process temperature	942:1051	The thermo gravimetric analysis indicates better thermal stability of the fiber up to 230°C, which is well within the polymerization process temperature.
24906775	3	25	theme	gravimetric	469:479	arg1	analysis					481:488	thermo gravimetric analysis	462:488	thermo gravimetric analysis	462:488	Comprehensive characterization of Cissus quadrangularis root fiber such as anatomical study, chemical analysis, physical analysis, FTIR, XRD, SEM analysis and thermo gravimetric analysis are done.
24906775	0	26	theme	new	20:22	arg1	fiber					43:47	new natural cellulosic fiber	20:47	new natural cellulosic fiber from Cissus quadrangularis root	20:79	Characterization of new natural cellulosic fiber from Cissus quadrangularis root.
24906775	4	27	theme	composite	574:582	arg1	application					541:551	its application	537:551	its application in fiber industries	537:571	The results are very encouraging for its application in fiber industries, composite manufacturing, etc.
24906775	4	27	theme	composite	574:582	arg1	manufacturing					584:596	composite manufacturing	574:596	composite manufacturing	574:596	The results are very encouraging for its application in fiber industries, composite manufacturing, etc.
24906775	3	28	dep	Cissus	337:342	arg1	quadrangularis					344:357	quadrangularis	344:357	quadrangularis	344:357	Comprehensive characterization of Cissus quadrangularis root fiber such as anatomical study, chemical analysis, physical analysis, FTIR, XRD, SEM analysis and thermo gravimetric analysis are done.
24906775	6	29	theme	low	807:809	arg1	angle					823:827	low microfibril angle	807:827	low microfibril angle	807:827	The flaky honeycomb outer surface and low microfibril angle revealed through electron microscopy contributes for its high modulus.
24906775	7	30	theme	better	942:947	arg1	stability					957:965	better thermal stability	942:965	better thermal stability of the fiber up to 230°C, which is well within the polymerization process temperature	942:1051	The thermo gravimetric analysis indicates better thermal stability of the fiber up to 230°C, which is well within the polymerization process temperature.
24906775	0	31	theme	fiber	43:47	arg1	Characterization					0:15	Characterization	0:15	Characterization of new natural cellulosic fiber from Cissus quadrangularis root.	0:80	Characterization of new natural cellulosic fiber from Cissus quadrangularis root.
24906775	5	32	with	presence	636:643	arg1	%					706:706	0.14%	702:706	0.14%	702:706	Due to its light weight and the presence of high cellulose content (77.17%) with very little wax (0.14%) provide high specific strength and good bonding properties.
24906775	5	32	with	presence	636:643	arg1	wax					697:699	very little wax	685:699	very little wax (0.14%)	685:707	Due to its light weight and the presence of high cellulose content (77.17%) with very little wax (0.14%) provide high specific strength and good bonding properties.
24906775	5	33	theme	little	690:695	arg1	%					706:706	0.14%	702:706	0.14%	702:706	Due to its light weight and the presence of high cellulose content (77.17%) with very little wax (0.14%) provide high specific strength and good bonding properties.
24906775	5	33	theme	little	690:695	arg1	wax					697:699	very little wax	685:699	very little wax (0.14%)	685:707	Due to its light weight and the presence of high cellulose content (77.17%) with very little wax (0.14%) provide high specific strength and good bonding properties.
24906775	0	34	theme	cellulosic	32:41	arg1	fiber					43:47	new natural cellulosic fiber	20:47	new natural cellulosic fiber from Cissus quadrangularis root	20:79	Characterization of new natural cellulosic fiber from Cissus quadrangularis root.
24906775	4	35	from	application	541:551	arg1	industries					562:571	fiber industries	556:571	fiber industries	556:571	The results are very encouraging for its application in fiber industries, composite manufacturing, etc.
24906775	0	36	from	root	76:79	arg1	fiber					43:47	new natural cellulosic fiber	20:47	new natural cellulosic fiber from Cissus quadrangularis root	20:79	Characterization of new natural cellulosic fiber from Cissus quadrangularis root.
24906775	0	36	from	root	76:79	arg1	Characterization					0:15	Characterization	0:15	Characterization of new natural cellulosic fiber from Cissus quadrangularis root.	0:80	Characterization of new natural cellulosic fiber from Cissus quadrangularis root.
24906775	3	37	theme	chemical	396:403	arg1	analysis					405:412	chemical analysis	396:412	chemical analysis	396:412	Comprehensive characterization of Cissus quadrangularis root fiber such as anatomical study, chemical analysis, physical analysis, FTIR, XRD, SEM analysis and thermo gravimetric analysis are done.
24906775	3	38	theme	thermo	462:467	arg1	analysis					481:488	thermo gravimetric analysis	462:488	thermo gravimetric analysis	462:488	Comprehensive characterization of Cissus quadrangularis root fiber such as anatomical study, chemical analysis, physical analysis, FTIR, XRD, SEM analysis and thermo gravimetric analysis are done.
24906775	0	39	theme	Cissus	54:59	arg1	root					76:79	Cissus quadrangularis root	54:79	Cissus quadrangularis root	54:79	Characterization of new natural cellulosic fiber from Cissus quadrangularis root.
24906775	3	40	theme	SEM	445:447	arg1	analysis					449:456	SEM analysis	445:456	SEM analysis	445:456	Comprehensive characterization of Cissus quadrangularis root fiber such as anatomical study, chemical analysis, physical analysis, FTIR, XRD, SEM analysis and thermo gravimetric analysis are done.
24906775	2	41	theme	E-glass	235:241	arg1	fibers					243:248	commonly used man-made E-glass fibers	212:248	commonly used man-made E-glass fibers	212:248	However commonly used man-made E-glass fibers are hazardous for health and carcinogenic by nature.
24906775	1	42	theme	replacing	122:130	arg1	structures					146:155	replacing many metallic structures	122:155	replacing many metallic structures due to its high specific strength and modulus	122:201	Fiber reinforced polymer composites are replacing many metallic structures due to its high specific strength and modulus.
24906775	1	42	theme	replacing	122:130	arg1	composites					107:116	Fiber reinforced polymer composites	82:116	Fiber reinforced polymer composites	82:116	Fiber reinforced polymer composites are replacing many metallic structures due to its high specific strength and modulus.
24906775	7	43	theme	process	1033:1039	arg1	temperature					1041:1051	the polymerization process temperature	1014:1051	the polymerization process temperature	1014:1051	The thermo gravimetric analysis indicates better thermal stability of the fiber up to 230°C, which is well within the polymerization process temperature.
24906775	3	44	theme	Cissus	337:342	arg1	fiber					364:368	Cissus quadrangularis root fiber	337:368	Cissus quadrangularis root fiber	337:368	Comprehensive characterization of Cissus quadrangularis root fiber such as anatomical study, chemical analysis, physical analysis, FTIR, XRD, SEM analysis and thermo gravimetric analysis are done.
24906775	2	45	theme	used	221:224	arg1	fibers					243:248	commonly used man-made E-glass fibers	212:248	commonly used man-made E-glass fibers	212:248	However commonly used man-made E-glass fibers are hazardous for health and carcinogenic by nature.
24906775	2	46	theme	man-made	226:233	arg1	fibers					243:248	commonly used man-made E-glass fibers	212:248	commonly used man-made E-glass fibers	212:248	However commonly used man-made E-glass fibers are hazardous for health and carcinogenic by nature.
24906775	1	47	theme	many	132:135	arg1	structures					146:155	replacing many metallic structures	122:155	replacing many metallic structures due to its high specific strength and modulus	122:201	Fiber reinforced polymer composites are replacing many metallic structures due to its high specific strength and modulus.
24906775	1	47	theme	many	132:135	arg1	composites					107:116	Fiber reinforced polymer composites	82:116	Fiber reinforced polymer composites	82:116	Fiber reinforced polymer composites are replacing many metallic structures due to its high specific strength and modulus.
24906775	3	48	theme	Comprehensive	303:315	arg1	characterization					317:332	Comprehensive characterization	303:332	Comprehensive characterization of Cissus quadrangularis root fiber such as anatomical study	303:393	Comprehensive characterization of Cissus quadrangularis root fiber such as anatomical study, chemical analysis, physical analysis, FTIR, XRD, SEM analysis and thermo gravimetric analysis are done.
24906775	7	49	theme	gravimetric	911:921	arg1	analysis					923:930	The thermo gravimetric analysis	900:930	The thermo gravimetric analysis	900:930	The thermo gravimetric analysis indicates better thermal stability of the fiber up to 230°C, which is well within the polymerization process temperature.
24906775	5	50	theme	high	717:720	arg1	strength					731:738	high specific strength	717:738	high specific strength	717:738	Due to its light weight and the presence of high cellulose content (77.17%) with very little wax (0.14%) provide high specific strength and good bonding properties.
24906775	1	51	theme	metallic	137:144	arg1	structures					146:155	replacing many metallic structures	122:155	replacing many metallic structures due to its high specific strength and modulus	122:201	Fiber reinforced polymer composites are replacing many metallic structures due to its high specific strength and modulus.
24906775	1	51	theme	metallic	137:144	arg1	composites					107:116	Fiber reinforced polymer composites	82:116	Fiber reinforced polymer composites	82:116	Fiber reinforced polymer composites are replacing many metallic structures due to its high specific strength and modulus.
24906775	7	52	theme	fiber	974:978	arg1	up					980:981	the fiber up	970:981	the fiber up to 230°C, which is well within the polymerization process temperature	970:1051	The thermo gravimetric analysis indicates better thermal stability of the fiber up to 230°C, which is well within the polymerization process temperature.
24906775	5	53	with	weight	621:626	arg1	%					706:706	0.14%	702:706	0.14%	702:706	Due to its light weight and the presence of high cellulose content (77.17%) with very little wax (0.14%) provide high specific strength and good bonding properties.
24906775	5	53	with	weight	621:626	arg1	wax					697:699	very little wax	685:699	very little wax (0.14%)	685:707	Due to its light weight and the presence of high cellulose content (77.17%) with very little wax (0.14%) provide high specific strength and good bonding properties.
24906775	3	54	theme	fiber	364:368	arg1	characterization					317:332	Comprehensive characterization	303:332	Comprehensive characterization of Cissus quadrangularis root fiber such as anatomical study	303:393	Comprehensive characterization of Cissus quadrangularis root fiber such as anatomical study, chemical analysis, physical analysis, FTIR, XRD, SEM analysis and thermo gravimetric analysis are done.
24906775	3	54	theme	fiber	364:368	arg1	XRD					440:442	XRD	440:442	XRD	440:442	Comprehensive characterization of Cissus quadrangularis root fiber such as anatomical study, chemical analysis, physical analysis, FTIR, XRD, SEM analysis and thermo gravimetric analysis are done.
24906775	3	54	theme	fiber	364:368	arg1	analysis					449:456	SEM analysis	445:456	SEM analysis	445:456	Comprehensive characterization of Cissus quadrangularis root fiber such as anatomical study, chemical analysis, physical analysis, FTIR, XRD, SEM analysis and thermo gravimetric analysis are done.
24906775	3	54	theme	fiber	364:368	arg1	analysis					424:431	physical analysis	415:431	physical analysis	415:431	Comprehensive characterization of Cissus quadrangularis root fiber such as anatomical study, chemical analysis, physical analysis, FTIR, XRD, SEM analysis and thermo gravimetric analysis are done.
24906775	3	54	theme	fiber	364:368	arg1	FTIR					434:437	FTIR	434:437	FTIR	434:437	Comprehensive characterization of Cissus quadrangularis root fiber such as anatomical study, chemical analysis, physical analysis, FTIR, XRD, SEM analysis and thermo gravimetric analysis are done.
24906775	3	54	theme	fiber	364:368	arg1	analysis					405:412	chemical analysis	396:412	chemical analysis	396:412	Comprehensive characterization of Cissus quadrangularis root fiber such as anatomical study, chemical analysis, physical analysis, FTIR, XRD, SEM analysis and thermo gravimetric analysis are done.
24906775	3	54	theme	fiber	364:368	arg1	analysis					481:488	thermo gravimetric analysis	462:488	thermo gravimetric analysis	462:488	Comprehensive characterization of Cissus quadrangularis root fiber such as anatomical study, chemical analysis, physical analysis, FTIR, XRD, SEM analysis and thermo gravimetric analysis are done.
24906775	6	55	theme	outer	789:793	arg1	surface					795:801	The flaky honeycomb outer surface	769:801	The flaky honeycomb outer surface	769:801	The flaky honeycomb outer surface and low microfibril angle revealed through electron microscopy contributes for its high modulus.
24906775	7	56	theme	up	980:981	arg1	stability					957:965	better thermal stability	942:965	better thermal stability of the fiber up to 230°C, which is well within the polymerization process temperature	942:1051	The thermo gravimetric analysis indicates better thermal stability of the fiber up to 230°C, which is well within the polymerization process temperature.
24906775	1	57	theme	due	157:159	arg1	structures					146:155	replacing many metallic structures	122:155	replacing many metallic structures due to its high specific strength and modulus	122:201	Fiber reinforced polymer composites are replacing many metallic structures due to its high specific strength and modulus.
24906775	1	57	theme	due	157:159	arg1	composites					107:116	Fiber reinforced polymer composites	82:116	Fiber reinforced polymer composites	82:116	Fiber reinforced polymer composites are replacing many metallic structures due to its high specific strength and modulus.
24906775	0	58	theme	quadrangularis	61:74	arg1	root					76:79	Cissus quadrangularis root	54:79	Cissus quadrangularis root	54:79	Characterization of new natural cellulosic fiber from Cissus quadrangularis root.
24906775	5	59	theme	good	744:747	arg1	properties					757:766	good bonding properties	744:766	good bonding properties	744:766	Due to its light weight and the presence of high cellulose content (77.17%) with very little wax (0.14%) provide high specific strength and good bonding properties.
26454110	0	0	theme	compost	91:97	arg1	characterization					12:27	characterization	12:27	characterization	12:27	Extraction, characterization and antioxidant activity of polysaccharides of spent mushroom compost of Ganoderma lucidum.
26454110	0	0	theme	compost	91:97	arg1	activity					45:52	antioxidant activity	33:52	antioxidant activity	33:52	Extraction, characterization and antioxidant activity of polysaccharides of spent mushroom compost of Ganoderma lucidum.
26454110	0	0	theme	compost	91:97	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction, characterization and antioxidant activity of polysaccharides of spent mushroom compost of Ganoderma lucidum.
26454110	5	1	theme	SMC-G	1049:1053	arg1	exploitation					1033:1044	the exploitation	1029:1044	the exploitation of SMC-G which would be significant to sustainable development of industry and agriculture, environmental protection and full utilization of resources	1029:1195	The results provided a reference for the exploitation of SMC-G which would be significant to sustainable development of industry and agriculture, environmental protection and full utilization of resources.
26454110	4	2	theme	potential	745:753	arg1	activities					767:776	potential antioxidant activities	745:776	potential antioxidant activities	745:776	Both GPS and its fractions showed potential antioxidant activities by scavenging hydroxyl and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, and increasing the reducing power in vitro; and by improving the CAT activities, and lowing the LPO and MDA contents in vivo, respectively.
26454110	5	3	theme	agriculture	1125:1135	arg1	development					1097:1107	sustainable development	1085:1107	sustainable development of industry and agriculture	1085:1135	The results provided a reference for the exploitation of SMC-G which would be significant to sustainable development of industry and agriculture, environmental protection and full utilization of resources.
26454110	5	3	theme	agriculture	1125:1135	arg1	protection					1152:1161	environmental protection	1138:1161	environmental protection	1138:1161	The results provided a reference for the exploitation of SMC-G which would be significant to sustainable development of industry and agriculture, environmental protection and full utilization of resources.
26454110	5	3	theme	agriculture	1125:1135	arg1	utilization					1172:1182	full utilization	1167:1182	full utilization of resources	1167:1195	The results provided a reference for the exploitation of SMC-G which would be significant to sustainable development of industry and agriculture, environmental protection and full utilization of resources.
26454110	0	4	theme	mushroom	82:89	arg1	compost					91:97	spent mushroom compost	76:97	spent mushroom compost of Ganoderma lucidum	76:118	Extraction, characterization and antioxidant activity of polysaccharides of spent mushroom compost of Ganoderma lucidum.
26454110	2	5	theme	34.5	525:528	arg1	material					513:520	material	513:520	material of 34.5	513:528	The optimum conditions for GPS extraction were optimized by the central composite design (CCD) and the GPS yield reached 3.84% at a ratio of water to material of 34.5, a precipitation time of 19.82h, and pH of 7.88.
26454110	2	5	theme	34.5	525:528	arg1	pH					567:568	pH	567:568	pH of 7.88	567:576	The optimum conditions for GPS extraction were optimized by the central composite design (CCD) and the GPS yield reached 3.84% at a ratio of water to material of 34.5, a precipitation time of 19.82h, and pH of 7.88.
26454110	2	5	theme	34.5	525:528	arg1	time					547:550	a precipitation time	531:550	a precipitation time of 19.82h	531:560	The optimum conditions for GPS extraction were optimized by the central composite design (CCD) and the GPS yield reached 3.84% at a ratio of water to material of 34.5, a precipitation time of 19.82h, and pH of 7.88.
26454110	0	6	theme	Ganoderma	102:110	arg1	lucidum					112:118	Ganoderma lucidum	102:118	Ganoderma lucidum	102:118	Extraction, characterization and antioxidant activity of polysaccharides of spent mushroom compost of Ganoderma lucidum.
26454110	5	7	theme	environmental	1138:1150	arg1	protection					1152:1161	environmental protection	1138:1161	environmental protection	1138:1161	The results provided a reference for the exploitation of SMC-G which would be significant to sustainable development of industry and agriculture, environmental protection and full utilization of resources.
26454110	1	8	theme	water-soluble	245:257	arg1	polysaccharide					259:272	a water-soluble polysaccharide	243:272	a water-soluble polysaccharide of GPS	243:279	To contribute toward effective exploitation and utilization of spent mushroom compost (SMC) of Ganoderma lucidum (SMC-G), a water-soluble polysaccharide of GPS was extracted, and then two fractions (GPS-1 and GPS-2) were purified from SMC-G.
26454110	3	9	dep	structures	678:687	arg1	CO					698:699	CO	698:699	CO	698:699	Characteristic analysis showed that GPS-1 and GPS-2 were heteropolysaccharides, and had glycosidic structures (OH, CH, CO and COC).
26454110	3	9	dep	structures	678:687	arg1	CH					694:695	CH	694:695	CH	694:695	Characteristic analysis showed that GPS-1 and GPS-2 were heteropolysaccharides, and had glycosidic structures (OH, CH, CO and COC).
26454110	3	9	dep	structures	678:687	arg1	OH					690:691	OH	690:691	OH	690:691	Characteristic analysis showed that GPS-1 and GPS-2 were heteropolysaccharides, and had glycosidic structures (OH, CH, CO and COC).
26454110	3	9	dep	structures	678:687	arg1	structures					678:687	glycosidic structures	667:687	glycosidic structures (OH, CH, CO and COC)	667:708	Characteristic analysis showed that GPS-1 and GPS-2 were heteropolysaccharides, and had glycosidic structures (OH, CH, CO and COC).
26454110	3	9	dep	structures	678:687	arg1	COC					705:707	COC	705:707	COC	705:707	Characteristic analysis showed that GPS-1 and GPS-2 were heteropolysaccharides, and had glycosidic structures (OH, CH, CO and COC).
26454110	4	10	theme	antioxidant	755:765	arg1	activities					767:776	potential antioxidant activities	745:776	potential antioxidant activities	745:776	Both GPS and its fractions showed potential antioxidant activities by scavenging hydroxyl and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, and increasing the reducing power in vitro; and by improving the CAT activities, and lowing the LPO and MDA contents in vivo, respectively.
26454110	2	11	theme	GPS	466:468	arg1	yield					470:474	the GPS yield	462:474	the GPS yield	462:474	The optimum conditions for GPS extraction were optimized by the central composite design (CCD) and the GPS yield reached 3.84% at a ratio of water to material of 34.5, a precipitation time of 19.82h, and pH of 7.88.
26454110	2	12	theme	19.82h	555:560	arg1	material					513:520	material	513:520	material of 34.5	513:528	The optimum conditions for GPS extraction were optimized by the central composite design (CCD) and the GPS yield reached 3.84% at a ratio of water to material of 34.5, a precipitation time of 19.82h, and pH of 7.88.
26454110	2	12	theme	19.82h	555:560	arg1	pH					567:568	pH	567:568	pH of 7.88	567:576	The optimum conditions for GPS extraction were optimized by the central composite design (CCD) and the GPS yield reached 3.84% at a ratio of water to material of 34.5, a precipitation time of 19.82h, and pH of 7.88.
26454110	2	12	theme	19.82h	555:560	arg1	time					547:550	a precipitation time	531:550	a precipitation time of 19.82h	531:560	The optimum conditions for GPS extraction were optimized by the central composite design (CCD) and the GPS yield reached 3.84% at a ratio of water to material of 34.5, a precipitation time of 19.82h, and pH of 7.88.
26454110	1	13	theme	spent	184:188	arg1	compost					199:205	spent mushroom compost	184:205	spent mushroom compost (SMC) of Ganoderma lucidum (SMC-G)	184:240	To contribute toward effective exploitation and utilization of spent mushroom compost (SMC) of Ganoderma lucidum (SMC-G), a water-soluble polysaccharide of GPS was extracted, and then two fractions (GPS-1 and GPS-2) were purified from SMC-G.
26454110	1	13	theme	spent	184:188	arg1	SMC					208:210	SMC	208:210	SMC	208:210	To contribute toward effective exploitation and utilization of spent mushroom compost (SMC) of Ganoderma lucidum (SMC-G), a water-soluble polysaccharide of GPS was extracted, and then two fractions (GPS-1 and GPS-2) were purified from SMC-G.
26454110	0	14	theme	lucidum	112:118	arg1	compost					91:97	spent mushroom compost	76:97	spent mushroom compost of Ganoderma lucidum	76:118	Extraction, characterization and antioxidant activity of polysaccharides of spent mushroom compost of Ganoderma lucidum.
26454110	1	15	theme	mushroom	190:197	arg1	compost					199:205	spent mushroom compost	184:205	spent mushroom compost (SMC) of Ganoderma lucidum (SMC-G)	184:240	To contribute toward effective exploitation and utilization of spent mushroom compost (SMC) of Ganoderma lucidum (SMC-G), a water-soluble polysaccharide of GPS was extracted, and then two fractions (GPS-1 and GPS-2) were purified from SMC-G.
26454110	1	15	theme	mushroom	190:197	arg1	SMC					208:210	SMC	208:210	SMC	208:210	To contribute toward effective exploitation and utilization of spent mushroom compost (SMC) of Ganoderma lucidum (SMC-G), a water-soluble polysaccharide of GPS was extracted, and then two fractions (GPS-1 and GPS-2) were purified from SMC-G.
26454110	2	16	theme	precipitation	533:545	arg1	time					547:550	a precipitation time	531:550	a precipitation time of 19.82h	531:560	The optimum conditions for GPS extraction were optimized by the central composite design (CCD) and the GPS yield reached 3.84% at a ratio of water to material of 34.5, a precipitation time of 19.82h, and pH of 7.88.
26454110	4	17	theme	reducing	871:878	arg1	power					880:884	the reducing power	867:884	the reducing power in vitro	867:893	Both GPS and its fractions showed potential antioxidant activities by scavenging hydroxyl and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, and increasing the reducing power in vitro; and by improving the CAT activities, and lowing the LPO and MDA contents in vivo, respectively.
26454110	1	18	theme	compost	199:205	arg1	utilization					169:179	utilization	169:179	utilization	169:179	To contribute toward effective exploitation and utilization of spent mushroom compost (SMC) of Ganoderma lucidum (SMC-G), a water-soluble polysaccharide of GPS was extracted, and then two fractions (GPS-1 and GPS-2) were purified from SMC-G.
26454110	1	18	theme	compost	199:205	arg1	exploitation					152:163	effective exploitation	142:163	effective exploitation	142:163	To contribute toward effective exploitation and utilization of spent mushroom compost (SMC) of Ganoderma lucidum (SMC-G), a water-soluble polysaccharide of GPS was extracted, and then two fractions (GPS-1 and GPS-2) were purified from SMC-G.
26454110	2	19	theme	7.88	573:576	arg1	material					513:520	material	513:520	material of 34.5	513:528	The optimum conditions for GPS extraction were optimized by the central composite design (CCD) and the GPS yield reached 3.84% at a ratio of water to material of 34.5, a precipitation time of 19.82h, and pH of 7.88.
26454110	2	19	theme	7.88	573:576	arg1	pH					567:568	pH	567:568	pH of 7.88	567:576	The optimum conditions for GPS extraction were optimized by the central composite design (CCD) and the GPS yield reached 3.84% at a ratio of water to material of 34.5, a precipitation time of 19.82h, and pH of 7.88.
26454110	2	19	theme	7.88	573:576	arg1	time					547:550	a precipitation time	531:550	a precipitation time of 19.82h	531:560	The optimum conditions for GPS extraction were optimized by the central composite design (CCD) and the GPS yield reached 3.84% at a ratio of water to material of 34.5, a precipitation time of 19.82h, and pH of 7.88.
26454110	1	20	theme	GPS	277:279	arg1	polysaccharide					259:272	a water-soluble polysaccharide	243:272	a water-soluble polysaccharide of GPS	243:279	To contribute toward effective exploitation and utilization of spent mushroom compost (SMC) of Ganoderma lucidum (SMC-G), a water-soluble polysaccharide of GPS was extracted, and then two fractions (GPS-1 and GPS-2) were purified from SMC-G.
26454110	5	21	theme	resources	1187:1195	arg1	development					1097:1107	sustainable development	1085:1107	sustainable development of industry and agriculture	1085:1135	The results provided a reference for the exploitation of SMC-G which would be significant to sustainable development of industry and agriculture, environmental protection and full utilization of resources.
26454110	5	21	theme	resources	1187:1195	arg1	protection					1152:1161	environmental protection	1138:1161	environmental protection	1138:1161	The results provided a reference for the exploitation of SMC-G which would be significant to sustainable development of industry and agriculture, environmental protection and full utilization of resources.
26454110	5	21	theme	resources	1187:1195	arg1	utilization					1172:1182	full utilization	1167:1182	full utilization of resources	1167:1195	The results provided a reference for the exploitation of SMC-G which would be significant to sustainable development of industry and agriculture, environmental protection and full utilization of resources.
26454110	2	22	theme	GPS	390:392	arg1	extraction					394:403	GPS extraction	390:403	GPS extraction	390:403	The optimum conditions for GPS extraction were optimized by the central composite design (CCD) and the GPS yield reached 3.84% at a ratio of water to material of 34.5, a precipitation time of 19.82h, and pH of 7.88.
26454110	4	23	theme	CAT	917:919	arg1	activities					921:930	the CAT activities	913:930	the CAT activities	913:930	Both GPS and its fractions showed potential antioxidant activities by scavenging hydroxyl and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, and increasing the reducing power in vitro; and by improving the CAT activities, and lowing the LPO and MDA contents in vivo, respectively.
26454110	1	24	dep	fractions	309:317	arg1	GPS-1					320:324	GPS-1	320:324	GPS-1	320:324	To contribute toward effective exploitation and utilization of spent mushroom compost (SMC) of Ganoderma lucidum (SMC-G), a water-soluble polysaccharide of GPS was extracted, and then two fractions (GPS-1 and GPS-2) were purified from SMC-G.
26454110	1	24	dep	fractions	309:317	arg1	fractions					309:317	two fractions	305:317	two fractions (GPS-1 and GPS-2)	305:335	To contribute toward effective exploitation and utilization of spent mushroom compost (SMC) of Ganoderma lucidum (SMC-G), a water-soluble polysaccharide of GPS was extracted, and then two fractions (GPS-1 and GPS-2) were purified from SMC-G.
26454110	1	24	dep	fractions	309:317	arg1	GPS-2					330:334	GPS-2	330:334	GPS-2	330:334	To contribute toward effective exploitation and utilization of spent mushroom compost (SMC) of Ganoderma lucidum (SMC-G), a water-soluble polysaccharide of GPS was extracted, and then two fractions (GPS-1 and GPS-2) were purified from SMC-G.
26454110	4	25	theme	1,1-diphenyl-2-picrylhydrazyl	805:833	arg1	radicals					842:849	scavenging hydroxyl and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals	781:849	radicals	842:849	Both GPS and its fractions showed potential antioxidant activities by scavenging hydroxyl and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, and increasing the reducing power in vitro; and by improving the CAT activities, and lowing the LPO and MDA contents in vivo, respectively.
26454110	3	26	contain	had	663:665	arg2	CH					694:695	CH	694:695	CH	694:695	Characteristic analysis showed that GPS-1 and GPS-2 were heteropolysaccharides, and had glycosidic structures (OH, CH, CO and COC).
26454110	3	26	contain	had	663:665	arg1	GPS-1					615:619	GPS-1	615:619	GPS-1	615:619	Characteristic analysis showed that GPS-1 and GPS-2 were heteropolysaccharides, and had glycosidic structures (OH, CH, CO and COC).
26454110	3	26	contain	had	663:665	arg2	structures					678:687	glycosidic structures	667:687	glycosidic structures (OH, CH, CO and COC)	667:708	Characteristic analysis showed that GPS-1 and GPS-2 were heteropolysaccharides, and had glycosidic structures (OH, CH, CO and COC).
26454110	3	26	contain	had	663:665	arg2	CO					698:699	CO	698:699	CO	698:699	Characteristic analysis showed that GPS-1 and GPS-2 were heteropolysaccharides, and had glycosidic structures (OH, CH, CO and COC).
26454110	3	26	contain	had	663:665	arg2	OH					690:691	OH	690:691	OH	690:691	Characteristic analysis showed that GPS-1 and GPS-2 were heteropolysaccharides, and had glycosidic structures (OH, CH, CO and COC).
26454110	3	26	contain	had	663:665	arg2	COC					705:707	COC	705:707	COC	705:707	Characteristic analysis showed that GPS-1 and GPS-2 were heteropolysaccharides, and had glycosidic structures (OH, CH, CO and COC).
26454110	0	27	theme	antioxidant	33:43	arg1	activity					45:52	antioxidant activity	33:52	antioxidant activity	33:52	Extraction, characterization and antioxidant activity of polysaccharides of spent mushroom compost of Ganoderma lucidum.
26454110	2	28	theme	composite	435:443	arg1	CCD					453:455	CCD	453:455	CCD	453:455	The optimum conditions for GPS extraction were optimized by the central composite design (CCD) and the GPS yield reached 3.84% at a ratio of water to material of 34.5, a precipitation time of 19.82h, and pH of 7.88.
26454110	2	28	theme	composite	435:443	arg1	design					445:450	the central composite design	423:450	the central composite design (CCD)	423:456	The optimum conditions for GPS extraction were optimized by the central composite design (CCD) and the GPS yield reached 3.84% at a ratio of water to material of 34.5, a precipitation time of 19.82h, and pH of 7.88.
26454110	1	29	theme	Ganoderma	216:224	arg1	SMC-G					235:239	SMC-G	235:239	SMC-G	235:239	To contribute toward effective exploitation and utilization of spent mushroom compost (SMC) of Ganoderma lucidum (SMC-G), a water-soluble polysaccharide of GPS was extracted, and then two fractions (GPS-1 and GPS-2) were purified from SMC-G.
26454110	1	29	theme	Ganoderma	216:224	arg1	lucidum					226:232	Ganoderma lucidum	216:232	Ganoderma lucidum (SMC-G)	216:240	To contribute toward effective exploitation and utilization of spent mushroom compost (SMC) of Ganoderma lucidum (SMC-G), a water-soluble polysaccharide of GPS was extracted, and then two fractions (GPS-1 and GPS-2) were purified from SMC-G.
26454110	0	30	theme	polysaccharides	57:71	arg1	characterization					12:27	characterization	12:27	characterization	12:27	Extraction, characterization and antioxidant activity of polysaccharides of spent mushroom compost of Ganoderma lucidum.
26454110	0	30	theme	polysaccharides	57:71	arg1	activity					45:52	antioxidant activity	33:52	antioxidant activity	33:52	Extraction, characterization and antioxidant activity of polysaccharides of spent mushroom compost of Ganoderma lucidum.
26454110	0	30	theme	polysaccharides	57:71	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction, characterization and antioxidant activity of polysaccharides of spent mushroom compost of Ganoderma lucidum.
26454110	4	31	theme	scavenging	781:790	arg1	hydroxyl					792:799	scavenging hydroxyl and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals	781:849	hydroxyl	792:799	Both GPS and its fractions showed potential antioxidant activities by scavenging hydroxyl and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, and increasing the reducing power in vitro; and by improving the CAT activities, and lowing the LPO and MDA contents in vivo, respectively.
26454110	3	32	theme	glycosidic	667:676	arg1	CO					698:699	CO	698:699	CO	698:699	Characteristic analysis showed that GPS-1 and GPS-2 were heteropolysaccharides, and had glycosidic structures (OH, CH, CO and COC).
26454110	3	32	theme	glycosidic	667:676	arg1	CH					694:695	CH	694:695	CH	694:695	Characteristic analysis showed that GPS-1 and GPS-2 were heteropolysaccharides, and had glycosidic structures (OH, CH, CO and COC).
26454110	3	32	theme	glycosidic	667:676	arg1	OH					690:691	OH	690:691	OH	690:691	Characteristic analysis showed that GPS-1 and GPS-2 were heteropolysaccharides, and had glycosidic structures (OH, CH, CO and COC).
26454110	3	32	theme	glycosidic	667:676	arg1	COC					705:707	COC	705:707	COC	705:707	Characteristic analysis showed that GPS-1 and GPS-2 were heteropolysaccharides, and had glycosidic structures (OH, CH, CO and COC).
26454110	3	32	theme	glycosidic	667:676	arg1	structures					678:687	glycosidic structures	667:687	glycosidic structures (OH, CH, CO and COC)	667:708	Characteristic analysis showed that GPS-1 and GPS-2 were heteropolysaccharides, and had glycosidic structures (OH, CH, CO and COC).
26454110	4	33	theme	DPPH	836:839	arg1	radicals					842:849	scavenging hydroxyl and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals	781:849	radicals	842:849	Both GPS and its fractions showed potential antioxidant activities by scavenging hydroxyl and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, and increasing the reducing power in vitro; and by improving the CAT activities, and lowing the LPO and MDA contents in vivo, respectively.
26454110	2	34	theme	water	504:508	arg1	ratio					495:499	a ratio	493:499	a ratio of water to material of 34.5, a precipitation time of 19.82h, and pH of 7.88	493:576	The optimum conditions for GPS extraction were optimized by the central composite design (CCD) and the GPS yield reached 3.84% at a ratio of water to material of 34.5, a precipitation time of 19.82h, and pH of 7.88.
26454110	3	35	theme	Characteristic	579:592	arg1	analysis					594:601	Characteristic analysis	579:601	Characteristic analysis	579:601	Characteristic analysis showed that GPS-1 and GPS-2 were heteropolysaccharides, and had glycosidic structures (OH, CH, CO and COC).
26454110	4	36	theme	LPO	948:950	arg1	contents					960:967	the LPO and MDA contents	944:967	the LPO and MDA contents	944:967	Both GPS and its fractions showed potential antioxidant activities by scavenging hydroxyl and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, and increasing the reducing power in vitro; and by improving the CAT activities, and lowing the LPO and MDA contents in vivo, respectively.
26454110	1	37	theme	lucidum	226:232	arg1	compost					199:205	spent mushroom compost	184:205	spent mushroom compost (SMC) of Ganoderma lucidum (SMC-G)	184:240	To contribute toward effective exploitation and utilization of spent mushroom compost (SMC) of Ganoderma lucidum (SMC-G), a water-soluble polysaccharide of GPS was extracted, and then two fractions (GPS-1 and GPS-2) were purified from SMC-G.
26454110	1	37	theme	lucidum	226:232	arg1	SMC					208:210	SMC	208:210	SMC	208:210	To contribute toward effective exploitation and utilization of spent mushroom compost (SMC) of Ganoderma lucidum (SMC-G), a water-soluble polysaccharide of GPS was extracted, and then two fractions (GPS-1 and GPS-2) were purified from SMC-G.
26454110	1	38	theme	effective	142:150	arg1	exploitation					152:163	effective exploitation	142:163	effective exploitation	142:163	To contribute toward effective exploitation and utilization of spent mushroom compost (SMC) of Ganoderma lucidum (SMC-G), a water-soluble polysaccharide of GPS was extracted, and then two fractions (GPS-1 and GPS-2) were purified from SMC-G.
26454110	5	39	theme	full	1167:1170	arg1	utilization					1172:1182	full utilization	1167:1182	full utilization of resources	1167:1195	The results provided a reference for the exploitation of SMC-G which would be significant to sustainable development of industry and agriculture, environmental protection and full utilization of resources.
26454110	0	40	theme	spent	76:80	arg1	compost					91:97	spent mushroom compost	76:97	spent mushroom compost of Ganoderma lucidum	76:118	Extraction, characterization and antioxidant activity of polysaccharides of spent mushroom compost of Ganoderma lucidum.
26454110	2	41	theme	optimum	367:373	arg1	conditions					375:384	The optimum conditions	363:384	The optimum conditions for GPS extraction	363:403	The optimum conditions for GPS extraction were optimized by the central composite design (CCD) and the GPS yield reached 3.84% at a ratio of water to material of 34.5, a precipitation time of 19.82h, and pH of 7.88.
26454110	4	42	theme	MDA	956:958	arg1	contents					960:967	the LPO and MDA contents	944:967	the LPO and MDA contents	944:967	Both GPS and its fractions showed potential antioxidant activities by scavenging hydroxyl and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, and increasing the reducing power in vitro; and by improving the CAT activities, and lowing the LPO and MDA contents in vivo, respectively.
26454110	2	43	theme	central	427:433	arg1	CCD					453:455	CCD	453:455	CCD	453:455	The optimum conditions for GPS extraction were optimized by the central composite design (CCD) and the GPS yield reached 3.84% at a ratio of water to material of 34.5, a precipitation time of 19.82h, and pH of 7.88.
26454110	2	43	theme	central	427:433	arg1	design					445:450	the central composite design	423:450	the central composite design (CCD)	423:456	The optimum conditions for GPS extraction were optimized by the central composite design (CCD) and the GPS yield reached 3.84% at a ratio of water to material of 34.5, a precipitation time of 19.82h, and pH of 7.88.
26454110	5	44	theme	sustainable	1085:1095	arg1	development					1097:1107	sustainable development	1085:1107	sustainable development of industry and agriculture	1085:1135	The results provided a reference for the exploitation of SMC-G which would be significant to sustainable development of industry and agriculture, environmental protection and full utilization of resources.
26454110	5	45	theme	industry	1112:1119	arg1	development					1097:1107	sustainable development	1085:1107	sustainable development of industry and agriculture	1085:1135	The results provided a reference for the exploitation of SMC-G which would be significant to sustainable development of industry and agriculture, environmental protection and full utilization of resources.
26454110	5	45	theme	industry	1112:1119	arg1	protection					1152:1161	environmental protection	1138:1161	environmental protection	1138:1161	The results provided a reference for the exploitation of SMC-G which would be significant to sustainable development of industry and agriculture, environmental protection and full utilization of resources.
26454110	5	45	theme	industry	1112:1119	arg1	utilization					1172:1182	full utilization	1167:1182	full utilization of resources	1167:1195	The results provided a reference for the exploitation of SMC-G which would be significant to sustainable development of industry and agriculture, environmental protection and full utilization of resources.
28934870	0	0	theme	Berteroa	82:89	arg1	herb					101:104	Berteroa incana L. herb	82:104	Berteroa incana L. herb	82:104	A bioanalytical approach of chemical composition, bioactivity and cytotoxicity of Berteroa incana L. herb.
28934870	3	1	theme	acid	720:723	arg1	5.23 ± 0.12					725:735	sinapic acid 5.23 ± 0.12	712:735	sinapic acid 5.23 ± 0.12	712:735	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	4	2	theme	high	926:929	arg1	content					931:937	high content	926:937	high content in saccharides	926:952	The polysaccharide fraction showed high content in saccharides, especially in arabinose (312.22 ± 7.54 mg g-1 polysaccharide extract) and glucose (279.22 ± 5.59), and promising cytostatic effect.
28934870	1	3	from	zones	162:166	arg1	widespread					138:147	widespread	138:147	widespread	138:147	Berteroa incana is a wild herb widespread in temperate zones which was practically not studied for its biological effects.
28934870	1	4	theme	widespread	138:147	arg1	herb					133:136	a wild herb	126:136	a wild herb widespread in temperate zones which was practically not studied for its biological effects	126:227	Berteroa incana is a wild herb widespread in temperate zones which was practically not studied for its biological effects.
28934870	1	4	theme	widespread	138:147	arg1	incana					116:121	Berteroa incana	107:121	Berteroa incana	107:121	Berteroa incana is a wild herb widespread in temperate zones which was practically not studied for its biological effects.
28934870	3	5	theme	antibacterial	867:879	arg1	activity					881:888	absent antibacterial activity	860:888	absent antibacterial activity	860:888	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	4	6	from	content	931:937	arg1	saccharides					942:952	saccharides	942:952	saccharides	942:952	The polysaccharide fraction showed high content in saccharides, especially in arabinose (312.22 ± 7.54 mg g-1 polysaccharide extract) and glucose (279.22 ± 5.59), and promising cytostatic effect.
28934870	1	7	from	widespread	138:147	arg1	zones					162:166	temperate zones	152:166	temperate zones which was practically not studied for its biological effects	152:227	Berteroa incana is a wild herb widespread in temperate zones which was practically not studied for its biological effects.
28934870	2	8	from	content	297:303	arg1	polyphenols					308:318	polyphenols	308:318	polyphenols	308:318	Methanolic and aqueous extracts of B. incana were assessed for the content in polyphenols and the related antioxidant and antimicrobial activities and the polysaccharide extract for the content in saccharides and the associated cytostatic effect.
28934870	0	9	theme	L.	98:99	arg1	herb					101:104	Berteroa incana L. herb	82:104	Berteroa incana L. herb	82:104	A bioanalytical approach of chemical composition, bioactivity and cytotoxicity of Berteroa incana L. herb.
28934870	3	10	theme	mg100 g-1dry	648:659	arg1	material					674:681	the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material	593:681	the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW)	593:686	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	10	theme	mg100 g-1dry	648:659	arg1	DW					684:685	DW	684:685	DW	684:685	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	11	theme	methanolic	519:528	arg1	extracts					530:537	the methanolic extracts	515:537	the methanolic extracts of B. incana	515:550	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	12	theme	incana	545:550	arg1	extracts					530:537	the methanolic extracts	515:537	the methanolic extracts of B. incana	515:550	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	0	13	theme	incana	91:96	arg1	herb					101:104	Berteroa incana L. herb	82:104	Berteroa incana L. herb	82:104	A bioanalytical approach of chemical composition, bioactivity and cytotoxicity of Berteroa incana L. herb.
28934870	3	14	theme	ferulic	741:747	arg1	100 g-1DW					769:777	ferulic acid 5.05 ± 0.12 mg 100 g-1DW	741:777	ferulic acid 5.05 ± 0.12 mg 100 g-1DW	741:777	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	2	15	theme	incana	268:273	arg1	extracts					253:260	Methanolic and aqueous extracts	230:260	Methanolic and aqueous extracts of B. incana	230:273	Methanolic and aqueous extracts of B. incana were assessed for the content in polyphenols and the related antioxidant and antimicrobial activities and the polysaccharide extract for the content in saccharides and the associated cytostatic effect.
28934870	3	16	theme	absent	860:865	arg1	activity					881:888	absent antibacterial activity	860:888	absent antibacterial activity	860:888	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	17	theme	acid	749:752	arg1	100 g-1DW					769:777	ferulic acid 5.05 ± 0.12 mg 100 g-1DW	741:777	ferulic acid 5.05 ± 0.12 mg 100 g-1DW	741:777	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	18	dep	obtained	489:496	arg1	highlighted					498:508	highlighted	498:508	obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity	489:888	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	1	19	theme	temperate	152:160	arg1	zones					162:166	temperate zones	152:166	temperate zones which was practically not studied for its biological effects	152:227	Berteroa incana is a wild herb widespread in temperate zones which was practically not studied for its biological effects.
28934870	0	20	theme	herb	101:104	arg1	composition					37:47	chemical composition	28:47	chemical composition	28:47	A bioanalytical approach of chemical composition, bioactivity and cytotoxicity of Berteroa incana L. herb.
28934870	0	20	theme	herb	101:104	arg1	cytotoxicity					66:77	cytotoxicity	66:77	cytotoxicity	66:77	A bioanalytical approach of chemical composition, bioactivity and cytotoxicity of Berteroa incana L. herb.
28934870	0	20	theme	herb	101:104	arg1	bioactivity					50:60	bioactivity	50:60	bioactivity	50:60	A bioanalytical approach of chemical composition, bioactivity and cytotoxicity of Berteroa incana L. herb.
28934870	3	21	theme	5.23 ± 0.12	725:735	arg1	material					674:681	the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material	593:681	the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW)	593:686	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	21	theme	5.23 ± 0.12	725:735	arg1	amounts					569:575	moderate amounts	560:575	moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW	560:777	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	21	theme	5.23 ± 0.12	725:735	arg1	polyphenols					580:590	polyphenols	580:590	polyphenols	580:590	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	21	theme	5.23 ± 0.12	725:735	arg1	100 g-1DW					769:777	ferulic acid 5.05 ± 0.12 mg 100 g-1DW	741:777	ferulic acid 5.05 ± 0.12 mg 100 g-1DW	741:777	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	21	theme	5.23 ± 0.12	725:735	arg1	DW					684:685	DW	684:685	DW	684:685	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	21	theme	5.23 ± 0.12	725:735	arg1	5.23 ± 0.12					725:735	sinapic acid 5.23 ± 0.12	712:735	sinapic acid 5.23 ± 0.12	712:735	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	21	theme	5.23 ± 0.12	725:735	arg1	quercetin					689:697	quercetin 4.21 ± 0.05	689:709	quercetin 4.21 ± 0.05	689:709	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	4	22	theme	promising	1058:1066	arg1	effect					1079:1084	promising cytostatic effect	1058:1084	promising cytostatic effect	1058:1084	The polysaccharide fraction showed high content in saccharides, especially in arabinose (312.22 ± 7.54 mg g-1 polysaccharide extract) and glucose (279.22 ± 5.59), and promising cytostatic effect.
28934870	3	23	theme	correlated	785:794	arg1	13.40 ± 0.01 μg mL-1					834:853	IC50 13.40 ± 0.01 μg mL-1	829:853	IC50 13.40 ± 0.01 μg mL-1	829:853	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	23	theme	correlated	785:794	arg1	activities					817:826	correlated moderate antioxidant activities	785:826	correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1)	785:854	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	0	24	theme	bioanalytical	2:14	arg1	approach					16:23	A bioanalytical approach	0:23	A bioanalytical approach of chemical composition, bioactivity and cytotoxicity of Berteroa incana L. herb	0:104	A bioanalytical approach of chemical composition, bioactivity and cytotoxicity of Berteroa incana L. herb.
28934870	4	25	theme	polysaccharide	895:908	arg1	fraction					910:917	The polysaccharide fraction	891:917	The polysaccharide fraction	891:917	The polysaccharide fraction showed high content in saccharides, especially in arabinose (312.22 ± 7.54 mg g-1 polysaccharide extract) and glucose (279.22 ± 5.59), and promising cytostatic effect.
28934870	3	26	theme	polyphenols	580:590	arg1	material					674:681	the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material	593:681	the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW)	593:686	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	26	theme	polyphenols	580:590	arg1	amounts					569:575	moderate amounts	560:575	moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW	560:777	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	26	theme	polyphenols	580:590	arg1	polyphenols					580:590	polyphenols	580:590	polyphenols	580:590	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	26	theme	polyphenols	580:590	arg1	100 g-1DW					769:777	ferulic acid 5.05 ± 0.12 mg 100 g-1DW	741:777	ferulic acid 5.05 ± 0.12 mg 100 g-1DW	741:777	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	26	theme	polyphenols	580:590	arg1	DW					684:685	DW	684:685	DW	684:685	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	26	theme	polyphenols	580:590	arg1	5.23 ± 0.12					725:735	sinapic acid 5.23 ± 0.12	712:735	sinapic acid 5.23 ± 0.12	712:735	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	26	theme	polyphenols	580:590	arg1	quercetin					689:697	quercetin 4.21 ± 0.05	689:709	quercetin 4.21 ± 0.05	689:709	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	27	theme	moderate	560:567	arg1	material					674:681	the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material	593:681	the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW)	593:686	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	27	theme	moderate	560:567	arg1	amounts					569:575	moderate amounts	560:575	moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW	560:777	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	27	theme	moderate	560:567	arg1	polyphenols					580:590	polyphenols	580:590	polyphenols	580:590	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	27	theme	moderate	560:567	arg1	100 g-1DW					769:777	ferulic acid 5.05 ± 0.12 mg 100 g-1DW	741:777	ferulic acid 5.05 ± 0.12 mg 100 g-1DW	741:777	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	27	theme	moderate	560:567	arg1	DW					684:685	DW	684:685	DW	684:685	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	27	theme	moderate	560:567	arg1	5.23 ± 0.12					725:735	sinapic acid 5.23 ± 0.12	712:735	sinapic acid 5.23 ± 0.12	712:735	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	27	theme	moderate	560:567	arg1	quercetin					689:697	quercetin 4.21 ± 0.05	689:709	quercetin 4.21 ± 0.05	689:709	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	28	theme	5.05 ± 0.12 mg	754:767	arg1	100 g-1DW					769:777	ferulic acid 5.05 ± 0.12 mg 100 g-1DW	741:777	ferulic acid 5.05 ± 0.12 mg 100 g-1DW	741:777	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	29	theme	material	674:681	arg1	material					674:681	the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material	593:681	the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW)	593:686	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	29	theme	material	674:681	arg1	amounts					569:575	moderate amounts	560:575	moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW	560:777	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	29	theme	material	674:681	arg1	polyphenols					580:590	polyphenols	580:590	polyphenols	580:590	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	29	theme	material	674:681	arg1	100 g-1DW					769:777	ferulic acid 5.05 ± 0.12 mg 100 g-1DW	741:777	ferulic acid 5.05 ± 0.12 mg 100 g-1DW	741:777	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	29	theme	material	674:681	arg1	DW					684:685	DW	684:685	DW	684:685	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	29	theme	material	674:681	arg1	5.23 ± 0.12					725:735	sinapic acid 5.23 ± 0.12	712:735	sinapic acid 5.23 ± 0.12	712:735	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	29	theme	material	674:681	arg1	quercetin					689:697	quercetin 4.21 ± 0.05	689:709	quercetin 4.21 ± 0.05	689:709	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	1	30	theme	wild	128:131	arg1	herb					133:136	a wild herb	126:136	a wild herb widespread in temperate zones which was practically not studied for its biological effects	126:227	Berteroa incana is a wild herb widespread in temperate zones which was practically not studied for its biological effects.
28934870	1	30	theme	wild	128:131	arg1	incana					116:121	Berteroa incana	107:121	Berteroa incana	107:121	Berteroa incana is a wild herb widespread in temperate zones which was practically not studied for its biological effects.
28934870	0	31	theme	composition	37:47	arg1	approach					16:23	A bioanalytical approach	0:23	A bioanalytical approach of chemical composition, bioactivity and cytotoxicity of Berteroa incana L. herb	0:104	A bioanalytical approach of chemical composition, bioactivity and cytotoxicity of Berteroa incana L. herb.
28934870	3	32	theme	100 g-1DW	769:777	arg1	material					674:681	the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material	593:681	the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW)	593:686	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	32	theme	100 g-1DW	769:777	arg1	amounts					569:575	moderate amounts	560:575	moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW	560:777	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	32	theme	100 g-1DW	769:777	arg1	polyphenols					580:590	polyphenols	580:590	polyphenols	580:590	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	32	theme	100 g-1DW	769:777	arg1	100 g-1DW					769:777	ferulic acid 5.05 ± 0.12 mg 100 g-1DW	741:777	ferulic acid 5.05 ± 0.12 mg 100 g-1DW	741:777	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	32	theme	100 g-1DW	769:777	arg1	DW					684:685	DW	684:685	DW	684:685	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	32	theme	100 g-1DW	769:777	arg1	5.23 ± 0.12					725:735	sinapic acid 5.23 ± 0.12	712:735	sinapic acid 5.23 ± 0.12	712:735	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	32	theme	100 g-1DW	769:777	arg1	quercetin					689:697	quercetin 4.21 ± 0.05	689:709	quercetin 4.21 ± 0.05	689:709	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	4	33	theme	polysaccharide	1001:1014	arg1	extract					1016:1022	312.22 ± 7.54 mg g-1 polysaccharide extract	980:1022	312.22 ± 7.54 mg g-1 polysaccharide extract	980:1022	The polysaccharide fraction showed high content in saccharides, especially in arabinose (312.22 ± 7.54 mg g-1 polysaccharide extract) and glucose (279.22 ± 5.59), and promising cytostatic effect.
28934870	4	33	theme	polysaccharide	1001:1014	arg1	arabinose					969:977	arabinose	969:977	arabinose (312.22 ± 7.54 mg g-1 polysaccharide extract)	969:1023	The polysaccharide fraction showed high content in saccharides, especially in arabinose (312.22 ± 7.54 mg g-1 polysaccharide extract) and glucose (279.22 ± 5.59), and promising cytostatic effect.
28934870	4	34	theme	312.22 ± 7.54 mg g-1	980:999	arg1	extract					1016:1022	312.22 ± 7.54 mg g-1 polysaccharide extract	980:1022	312.22 ± 7.54 mg g-1 polysaccharide extract	980:1022	The polysaccharide fraction showed high content in saccharides, especially in arabinose (312.22 ± 7.54 mg g-1 polysaccharide extract) and glucose (279.22 ± 5.59), and promising cytostatic effect.
28934870	4	34	theme	312.22 ± 7.54 mg g-1	980:999	arg1	arabinose					969:977	arabinose	969:977	arabinose (312.22 ± 7.54 mg g-1 polysaccharide extract)	969:1023	The polysaccharide fraction showed high content in saccharides, especially in arabinose (312.22 ± 7.54 mg g-1 polysaccharide extract) and glucose (279.22 ± 5.59), and promising cytostatic effect.
28934870	2	35	theme	polysaccharide	385:398	arg1	extract					400:406	the polysaccharide extract	381:406	the polysaccharide extract	381:406	Methanolic and aqueous extracts of B. incana were assessed for the content in polyphenols and the related antioxidant and antimicrobial activities and the polysaccharide extract for the content in saccharides and the associated cytostatic effect.
28934870	3	36	theme	antioxidant	805:815	arg1	13.40 ± 0.01 μg mL-1					834:853	IC50 13.40 ± 0.01 μg mL-1	829:853	IC50 13.40 ± 0.01 μg mL-1	829:853	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	36	theme	antioxidant	805:815	arg1	activities					817:826	correlated moderate antioxidant activities	785:826	correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1)	785:854	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	0	37	theme	chemical	28:35	arg1	composition					37:47	chemical composition	28:47	chemical composition	28:47	A bioanalytical approach of chemical composition, bioactivity and cytotoxicity of Berteroa incana L. herb.
28934870	3	38	theme	weight	661:666	arg1	material					674:681	the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material	593:681	the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW)	593:686	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	38	theme	weight	661:666	arg1	DW					684:685	DW	684:685	DW	684:685	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	39	theme	representative	602:615	arg1	material					674:681	the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material	593:681	the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW)	593:686	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	39	theme	representative	602:615	arg1	DW					684:685	DW	684:685	DW	684:685	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	40	theme	moderate	796:803	arg1	13.40 ± 0.01 μg mL-1					834:853	IC50 13.40 ± 0.01 μg mL-1	829:853	IC50 13.40 ± 0.01 μg mL-1	829:853	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	40	theme	moderate	796:803	arg1	activities					817:826	correlated moderate antioxidant activities	785:826	correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1)	785:854	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	0	41	theme	bioactivity	50:60	arg1	approach					16:23	A bioanalytical approach	0:23	A bioanalytical approach of chemical composition, bioactivity and cytotoxicity of Berteroa incana L. herb	0:104	A bioanalytical approach of chemical composition, bioactivity and cytotoxicity of Berteroa incana L. herb.
28934870	3	42	theme	plant	668:672	arg1	material					674:681	the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material	593:681	the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW)	593:686	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	42	theme	plant	668:672	arg1	DW					684:685	DW	684:685	DW	684:685	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	2	43	from	activities	366:375	arg1	polyphenols					308:318	polyphenols	308:318	polyphenols	308:318	Methanolic and aqueous extracts of B. incana were assessed for the content in polyphenols and the related antioxidant and antimicrobial activities and the polysaccharide extract for the content in saccharides and the associated cytostatic effect.
28934870	1	44	theme	Berteroa	107:114	arg1	herb					133:136	a wild herb	126:136	a wild herb widespread in temperate zones which was practically not studied for its biological effects	126:227	Berteroa incana is a wild herb widespread in temperate zones which was practically not studied for its biological effects.
28934870	1	44	theme	Berteroa	107:114	arg1	incana					116:121	Berteroa incana	107:121	Berteroa incana	107:121	Berteroa incana is a wild herb widespread in temperate zones which was practically not studied for its biological effects.
28934870	2	45	theme	associated	447:456	arg1	effect					469:474	the associated cytostatic effect	443:474	the associated cytostatic effect	443:474	Methanolic and aqueous extracts of B. incana were assessed for the content in polyphenols and the related antioxidant and antimicrobial activities and the polysaccharide extract for the content in saccharides and the associated cytostatic effect.
28934870	3	46	contain	contain	552:558	arg2	DW					684:685	DW	684:685	DW	684:685	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	46	contain	contain	552:558	arg2	100 g-1DW					769:777	ferulic acid 5.05 ± 0.12 mg 100 g-1DW	741:777	ferulic acid 5.05 ± 0.12 mg 100 g-1DW	741:777	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	46	contain	contain	552:558	arg1	extracts					530:537	the methanolic extracts	515:537	the methanolic extracts of B. incana	515:550	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	46	contain	contain	552:558	arg2	quercetin					689:697	quercetin 4.21 ± 0.05	689:709	quercetin 4.21 ± 0.05	689:709	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	46	contain	contain	552:558	arg2	5.23 ± 0.12					725:735	sinapic acid 5.23 ± 0.12	712:735	sinapic acid 5.23 ± 0.12	712:735	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	46	contain	contain	552:558	arg2	amounts					569:575	moderate amounts	560:575	moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW	560:777	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	46	contain	contain	552:558	arg2	polyphenols					580:590	polyphenols	580:590	polyphenols	580:590	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	46	contain	contain	552:558	arg2	material					674:681	the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material	593:681	the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW)	593:686	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	4	47	theme	cytostatic	1068:1077	arg1	effect					1079:1084	promising cytostatic effect	1058:1084	promising cytostatic effect	1058:1084	The polysaccharide fraction showed high content in saccharides, especially in arabinose (312.22 ± 7.54 mg g-1 polysaccharide extract) and glucose (279.22 ± 5.59), and promising cytostatic effect.
28934870	3	48	theme	isoquercitrin	622:634	arg1	material					674:681	the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material	593:681	the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW)	593:686	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	48	theme	isoquercitrin	622:634	arg1	DW					684:685	DW	684:685	DW	684:685	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	49	theme	quercetin	689:697	arg1	material					674:681	the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material	593:681	the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW)	593:686	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	49	theme	quercetin	689:697	arg1	amounts					569:575	moderate amounts	560:575	moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW	560:777	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	49	theme	quercetin	689:697	arg1	polyphenols					580:590	polyphenols	580:590	polyphenols	580:590	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	49	theme	quercetin	689:697	arg1	100 g-1DW					769:777	ferulic acid 5.05 ± 0.12 mg 100 g-1DW	741:777	ferulic acid 5.05 ± 0.12 mg 100 g-1DW	741:777	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	49	theme	quercetin	689:697	arg1	DW					684:685	DW	684:685	DW	684:685	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	49	theme	quercetin	689:697	arg1	5.23 ± 0.12					725:735	sinapic acid 5.23 ± 0.12	712:735	sinapic acid 5.23 ± 0.12	712:735	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	49	theme	quercetin	689:697	arg1	quercetin					689:697	quercetin 4.21 ± 0.05	689:709	quercetin 4.21 ± 0.05	689:709	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	50	theme	4.41 ± 0.02	636:646	arg1	material					674:681	the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material	593:681	the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW)	593:686	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	50	theme	4.41 ± 0.02	636:646	arg1	DW					684:685	DW	684:685	DW	684:685	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	2	51	theme	aqueous	245:251	arg1	extracts					253:260	Methanolic and aqueous extracts	230:260	Methanolic and aqueous extracts of B. incana	230:273	Methanolic and aqueous extracts of B. incana were assessed for the content in polyphenols and the related antioxidant and antimicrobial activities and the polysaccharide extract for the content in saccharides and the associated cytostatic effect.
28934870	1	52	theme	biological	210:219	arg1	effects					221:227	its biological effects	206:227	its biological effects	206:227	Berteroa incana is a wild herb widespread in temperate zones which was practically not studied for its biological effects.
28934870	0	53	theme	cytotoxicity	66:77	arg1	approach					16:23	A bioanalytical approach	0:23	A bioanalytical approach of chemical composition, bioactivity and cytotoxicity of Berteroa incana L. herb	0:104	A bioanalytical approach of chemical composition, bioactivity and cytotoxicity of Berteroa incana L. herb.
28934870	3	54	theme	been	617:620	arg1	material					674:681	the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material	593:681	the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW)	593:686	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	54	theme	been	617:620	arg1	DW					684:685	DW	684:685	DW	684:685	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	2	55	from	extract	400:406	arg1	polyphenols					308:318	polyphenols	308:318	polyphenols	308:318	Methanolic and aqueous extracts of B. incana were assessed for the content in polyphenols and the related antioxidant and antimicrobial activities and the polysaccharide extract for the content in saccharides and the associated cytostatic effect.
28934870	2	56	theme	antimicrobial	352:364	arg1	activities					366:375	the related antioxidant and antimicrobial activities	324:375	activities	366:375	Methanolic and aqueous extracts of B. incana were assessed for the content in polyphenols and the related antioxidant and antimicrobial activities and the polysaccharide extract for the content in saccharides and the associated cytostatic effect.
28934870	2	57	theme	cytostatic	458:467	arg1	effect					469:474	the associated cytostatic effect	443:474	the associated cytostatic effect	443:474	Methanolic and aqueous extracts of B. incana were assessed for the content in polyphenols and the related antioxidant and antimicrobial activities and the polysaccharide extract for the content in saccharides and the associated cytostatic effect.
28934870	3	58	theme	sinapic	712:718	arg1	5.23 ± 0.12					725:735	sinapic acid 5.23 ± 0.12	712:735	sinapic acid 5.23 ± 0.12	712:735	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	2	59	theme	Methanolic	230:239	arg1	extracts					253:260	Methanolic and aqueous extracts	230:260	Methanolic and aqueous extracts of B. incana	230:273	Methanolic and aqueous extracts of B. incana were assessed for the content in polyphenols and the related antioxidant and antimicrobial activities and the polysaccharide extract for the content in saccharides and the associated cytostatic effect.
28934870	2	60	from	content	416:422	arg1	effect					469:474	the associated cytostatic effect	443:474	the associated cytostatic effect	443:474	Methanolic and aqueous extracts of B. incana were assessed for the content in polyphenols and the related antioxidant and antimicrobial activities and the polysaccharide extract for the content in saccharides and the associated cytostatic effect.
28934870	2	60	from	content	416:422	arg1	saccharides					427:437	saccharides	427:437	saccharides	427:437	Methanolic and aqueous extracts of B. incana were assessed for the content in polyphenols and the related antioxidant and antimicrobial activities and the polysaccharide extract for the content in saccharides and the associated cytostatic effect.
28934870	3	61	theme	IC50	829:832	arg1	13.40 ± 0.01 μg mL-1					834:853	IC50 13.40 ± 0.01 μg mL-1	829:853	IC50 13.40 ± 0.01 μg mL-1	829:853	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28934870	3	61	theme	IC50	829:832	arg1	activities					817:826	correlated moderate antioxidant activities	785:826	correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1)	785:854	The results obtained highlighted that the methanolic extracts of B. incana contain moderate amounts of polyphenols, the most representative been isoquercitrin 4.41 ± 0.02 mg100 g-1dry weight plant material (DW), quercetin 4.21 ± 0.05, sinapic acid 5.23 ± 0.12 and ferulic acid 5.05 ± 0.12 mg 100 g-1DW, with correlated moderate antioxidant activities (IC50 13.40 ± 0.01 μg mL-1) and absent antibacterial activity.
28318581	7	0	theme	root	1387:1390	arg1	square					1397:1402	z-average root mean square	1377:1402	z-average root mean square	1377:1402	The EPS from each strain was isolated and the weight-average molar mass and z-average root mean square radius determined using size-exclusion chromatography multiangle laser light scattering.
28318581	8	1	theme	chromatography-mass	1556:1574	arg1	spectrometry					1576:1587	gas chromatography-mass spectrometry	1552:1587	gas chromatography-mass spectrometry	1552:1587	The monosaccharide composition of EPS was determined using gas chromatography-mass spectrometry, and repeating unit structure was determined using nuclear magnetic resonance spectroscopy.
28318581	12	2	from	galactose	1965:1973	arg1	DGCC					2082:2085	DGCC 7785	2082:2090	DGCC 7785	2082:2090	The monosaccharide compositions of the various EPS were mainly composed of glucose and galactose, with low levels of rhamnose in the EPS isolated from DGCC 7698, and N-acetylgalactosamine in the EPS from DGCC 7785, ST-10255y, and ST4239.
28318581	12	2	from	galactose	1965:1973	arg1	ST-10255y					2093:2101	ST-10255y	2093:2101	ST-10255y	2093:2101	The monosaccharide compositions of the various EPS were mainly composed of glucose and galactose, with low levels of rhamnose in the EPS isolated from DGCC 7698, and N-acetylgalactosamine in the EPS from DGCC 7785, ST-10255y, and ST4239.
28318581	12	2	from	galactose	1965:1973	arg1	EPS					2073:2075	the EPS	2069:2075	the EPS from DGCC 7785, ST-10255y, and ST4239	2069:2113	The monosaccharide compositions of the various EPS were mainly composed of glucose and galactose, with low levels of rhamnose in the EPS isolated from DGCC 7698, and N-acetylgalactosamine in the EPS from DGCC 7785, ST-10255y, and ST4239.
28318581	12	2	from	galactose	1965:1973	arg1	ST4239					2108:2113	ST4239	2108:2113	ST4239	2108:2113	The monosaccharide compositions of the various EPS were mainly composed of glucose and galactose, with low levels of rhamnose in the EPS isolated from DGCC 7698, and N-acetylgalactosamine in the EPS from DGCC 7785, ST-10255y, and ST4239.
28318581	12	3	with	glucose	1953:1959	arg1	levels					1985:1990	low levels	1981:1990	low levels of rhamnose in the EPS isolated from DGCC 7698	1981:2037	The monosaccharide compositions of the various EPS were mainly composed of glucose and galactose, with low levels of rhamnose in the EPS isolated from DGCC 7698, and N-acetylgalactosamine in the EPS from DGCC 7785, ST-10255y, and ST4239.
28318581	8	4	theme	nuclear	1640:1646	arg1	resonance					1657:1665	nuclear magnetic resonance	1640:1665	nuclear magnetic resonance spectroscopy	1640:1678	The monosaccharide composition of EPS was determined using gas chromatography-mass spectrometry, and repeating unit structure was determined using nuclear magnetic resonance spectroscopy.
28318581	1	5	theme	rheological	261:271	arg1	properties					286:295	the rheological and physical properties	257:295	the rheological and physical properties of yogurt	257:305	Exopolysaccharides (EPS) produced by some lactic acid bacteria are often used by the dairy industry to improve the rheological and physical properties of yogurt, but the relationship between their structure and functional effect is still unclear.
28318581	12	6	from	glucose	1953:1959	arg1	DGCC					2082:2085	DGCC 7785	2082:2090	DGCC 7785	2082:2090	The monosaccharide compositions of the various EPS were mainly composed of glucose and galactose, with low levels of rhamnose in the EPS isolated from DGCC 7698, and N-acetylgalactosamine in the EPS from DGCC 7785, ST-10255y, and ST4239.
28318581	12	6	from	glucose	1953:1959	arg1	ST-10255y					2093:2101	ST-10255y	2093:2101	ST-10255y	2093:2101	The monosaccharide compositions of the various EPS were mainly composed of glucose and galactose, with low levels of rhamnose in the EPS isolated from DGCC 7698, and N-acetylgalactosamine in the EPS from DGCC 7785, ST-10255y, and ST4239.
28318581	12	6	from	glucose	1953:1959	arg1	EPS					2073:2075	the EPS	2069:2075	the EPS from DGCC 7785, ST-10255y, and ST4239	2069:2113	The monosaccharide compositions of the various EPS were mainly composed of glucose and galactose, with low levels of rhamnose in the EPS isolated from DGCC 7698, and N-acetylgalactosamine in the EPS from DGCC 7785, ST-10255y, and ST4239.
28318581	12	6	from	glucose	1953:1959	arg1	ST4239					2108:2113	ST4239	2108:2113	ST4239	2108:2113	The monosaccharide compositions of the various EPS were mainly composed of glucose and galactose, with low levels of rhamnose in the EPS isolated from DGCC 7698, and N-acetylgalactosamine in the EPS from DGCC 7785, ST-10255y, and ST4239.
28318581	2	7	theme	milk	567:570	arg1	fermentation					551:562	fermentation	551:562	fermentation of milk	551:570	The EPS from different species, or different strains from the same species, may differ in terms of molar mass, repeating unit structure, and EPS yield during fermentation of milk.
28318581	1	8	used	used	219:222	arg2	EPS					166:168	EPS	166:168	EPS	166:168	Exopolysaccharides (EPS) produced by some lactic acid bacteria are often used by the dairy industry to improve the rheological and physical properties of yogurt, but the relationship between their structure and functional effect is still unclear.
28318581	1	8	used	used	219:222	arg2	Exopolysaccharides					146:163	Exopolysaccharides	146:163	Exopolysaccharides (EPS) produced by some lactic acid bacteria	146:207	Exopolysaccharides (EPS) produced by some lactic acid bacteria are often used by the dairy industry to improve the rheological and physical properties of yogurt, but the relationship between their structure and functional effect is still unclear.
28318581	14	9	dep	EPS	2257:2259	arg1	addition					2238:2245	addition	2238:2245	addition	2238:2245	In addition to (free) EPS, some strains were also able to produce capsular polysaccharide (associated with the bacterial cells) when observed with negative staining technique.
28318581	8	10	theme	resonance	1657:1665	arg1	spectroscopy					1667:1678	nuclear magnetic resonance spectroscopy	1640:1678	nuclear magnetic resonance spectroscopy	1640:1678	The monosaccharide composition of EPS was determined using gas chromatography-mass spectrometry, and repeating unit structure was determined using nuclear magnetic resonance spectroscopy.
28318581	1	11	theme	physical	277:284	arg1	properties					286:295	the rheological and physical properties	257:295	the rheological and physical properties of yogurt	257:305	Exopolysaccharides (EPS) produced by some lactic acid bacteria are often used by the dairy industry to improve the rheological and physical properties of yogurt, but the relationship between their structure and functional effect is still unclear.
28318581	7	12	theme	molar	1362:1366	arg1	mass					1368:1371	the weight-average molar mass	1343:1371	the weight-average molar mass	1343:1371	The EPS from each strain was isolated and the weight-average molar mass and z-average root mean square radius determined using size-exclusion chromatography multiangle laser light scattering.
28318581	4	13	theme	DGCC	797:800	arg1	7710					802:805	DGCC 7710	797:805	DGCC 7710	797:805	Milk was fermented with strains DGCC 7698, DGCC 7710, DGCC 7785, ST-10255y, St-143, STCth-9204, and ST4239.
28318581	6	14	theme	similar	1081:1087	arg1	rate					1103:1106	a similar acidification rate	1079:1106	a similar acidification rate	1079:1106	All strains were fermented under a similar acidification rate by adjusting the level of supplementation with peptone or the inoculation level, which allowed for a comparison of EPS yields under similar growth conditions (reconstituted skim milk at 40°C).
28318581	12	15	theme	monosaccharide	1882:1895	arg1	compositions					1897:1908	The monosaccharide compositions	1878:1908	The monosaccharide compositions of the various EPS	1878:1927	The monosaccharide compositions of the various EPS were mainly composed of glucose and galactose, with low levels of rhamnose in the EPS isolated from DGCC 7698, and N-acetylgalactosamine in the EPS from DGCC 7785, ST-10255y, and ST4239.
28318581	6	16	theme	inoculation	1170:1180	arg1	level					1182:1186	the inoculation level	1166:1186	the inoculation level	1166:1186	All strains were fermented under a similar acidification rate by adjusting the level of supplementation with peptone or the inoculation level, which allowed for a comparison of EPS yields under similar growth conditions (reconstituted skim milk at 40°C).
28318581	1	17	theme	acid	195:198	arg1	bacteria					200:207	some lactic acid bacteria	183:207	some lactic acid bacteria	183:207	Exopolysaccharides (EPS) produced by some lactic acid bacteria are often used by the dairy industry to improve the rheological and physical properties of yogurt, but the relationship between their structure and functional effect is still unclear.
28318581	3	18	theme	key	712:714	arg1	cultures					716:723	the key cultures	708:723	the key cultures used for yogurt manufacture	708:751	This study aimed to characterize the detailed properties of EPS produced from 7 strains of Streptococcus thermophilus, which is one of the key cultures used for yogurt manufacture.
28318581	15	19	theme	different	2509:2517	arg1	strains					2519:2525	different strains	2509:2525	different strains of Streptococcus thermophilus	2509:2555	The results of our study will help the dairy industry to better understand the mechanism by which different strains of Streptococcus thermophilus affect yogurt texture.
28318581	13	20	theme	glucose	2211:2217	arg1	equivalents/kg					2219:2232	glucose equivalents/kg	2211:2232	glucose equivalents/kg	2211:2232	The yields of EPS (measured when fermented milks reached pH 4.6) ranged from 8.0 to 76.4 mg of glucose equivalents/kg.
28318581	12	21	theme	EPS	1925:1927	arg1	compositions					1897:1908	The monosaccharide compositions	1878:1908	The monosaccharide compositions of the various EPS	1878:1927	The monosaccharide compositions of the various EPS were mainly composed of glucose and galactose, with low levels of rhamnose in the EPS isolated from DGCC 7698, and N-acetylgalactosamine in the EPS from DGCC 7785, ST-10255y, and ST4239.
28318581	6	22	theme	similar	1240:1246	arg1	conditions					1255:1264	similar growth conditions	1240:1264	similar growth conditions	1240:1264	All strains were fermented under a similar acidification rate by adjusting the level of supplementation with peptone or the inoculation level, which allowed for a comparison of EPS yields under similar growth conditions (reconstituted skim milk at 40°C).
28318581	12	23	from	ST4239	2108:2113	arg1	N-acetylgalactosamine					2044:2064	N-acetylgalactosamine	2044:2064	N-acetylgalactosamine	2044:2064	The monosaccharide compositions of the various EPS were mainly composed of glucose and galactose, with low levels of rhamnose in the EPS isolated from DGCC 7698, and N-acetylgalactosamine in the EPS from DGCC 7785, ST-10255y, and ST4239.
28318581	12	23	from	ST4239	2108:2113	arg1	galactose					1965:1973	galactose	1965:1973	galactose	1965:1973	The monosaccharide compositions of the various EPS were mainly composed of glucose and galactose, with low levels of rhamnose in the EPS isolated from DGCC 7698, and N-acetylgalactosamine in the EPS from DGCC 7785, ST-10255y, and ST4239.
28318581	12	23	from	ST4239	2108:2113	arg1	EPS					2073:2075	the EPS	2069:2075	the EPS from DGCC 7785, ST-10255y, and ST4239	2069:2113	The monosaccharide compositions of the various EPS were mainly composed of glucose and galactose, with low levels of rhamnose in the EPS isolated from DGCC 7698, and N-acetylgalactosamine in the EPS from DGCC 7785, ST-10255y, and ST4239.
28318581	12	23	from	ST4239	2108:2113	arg1	glucose					1953:1959	glucose	1953:1959	glucose	1953:1959	The monosaccharide compositions of the various EPS were mainly composed of glucose and galactose, with low levels of rhamnose in the EPS isolated from DGCC 7698, and N-acetylgalactosamine in the EPS from DGCC 7785, ST-10255y, and ST4239.
28318581	2	24	theme	structure	519:527	arg1	terms					483:487	terms	483:487	terms of molar mass, repeating unit structure, and EPS yield	483:542	The EPS from different species, or different strains from the same species, may differ in terms of molar mass, repeating unit structure, and EPS yield during fermentation of milk.
28318581	3	25	theme	yogurt	734:739	arg1	manufacture					741:751	yogurt manufacture	734:751	yogurt manufacture	734:751	This study aimed to characterize the detailed properties of EPS produced from 7 strains of Streptococcus thermophilus, which is one of the key cultures used for yogurt manufacture.
28318581	6	26	theme	yields	1227:1232	arg1	comparison					1209:1218	a comparison	1207:1218	a comparison of EPS yields	1207:1232	All strains were fermented under a similar acidification rate by adjusting the level of supplementation with peptone or the inoculation level, which allowed for a comparison of EPS yields under similar growth conditions (reconstituted skim milk at 40°C).
28318581	5	27	theme	EPS	993:995	arg1	properties					1008:1017	their EPS structural properties	987:1017	their EPS structural properties	987:1017	These strains were selected because they have been used in previous studies on yogurt texture, but a complete description of their EPS structural properties has not yet been reported.
28318581	6	28	theme	supplementation	1134:1148	arg1	level					1125:1129	the level	1121:1129	the level of supplementation with peptone	1121:1161	All strains were fermented under a similar acidification rate by adjusting the level of supplementation with peptone or the inoculation level, which allowed for a comparison of EPS yields under similar growth conditions (reconstituted skim milk at 40°C).
28318581	6	28	theme	supplementation	1134:1148	arg1	level					1182:1186	the inoculation level	1166:1186	the inoculation level	1166:1186	All strains were fermented under a similar acidification rate by adjusting the level of supplementation with peptone or the inoculation level, which allowed for a comparison of EPS yields under similar growth conditions (reconstituted skim milk at 40°C).
28318581	9	29	theme	molar	1700:1704	arg1	values					1711:1716	The weight-average molar mass values	1681:1716	The weight-average molar mass values of EPS	1681:1723	The weight-average molar mass values of EPS ranged from 0.14 to 1.61 × 106 g/mol.
28318581	11	30	with	EPS	1834:1836	arg1	structure					1867:1875	the same repeating unit structure	1843:1875	the same repeating unit structure	1843:1875	The strains ST-10255y and ST4239 had EPS with the same repeating unit structure.
28318581	2	31	theme	yield	538:542	arg1	terms					483:487	terms	483:487	terms of molar mass, repeating unit structure, and EPS yield	483:542	The EPS from different species, or different strains from the same species, may differ in terms of molar mass, repeating unit structure, and EPS yield during fermentation of milk.
28318581	5	32	theme	complete	963:970	arg1	description					972:982	a complete description	961:982	a complete description of their EPS structural properties	961:1017	These strains were selected because they have been used in previous studies on yogurt texture, but a complete description of their EPS structural properties has not yet been reported.
28318581	10	33	theme	EPS	1769:1771	arg1	samples					1773:1779	All 7 EPS samples	1763:1779	All 7 EPS samples	1763:1779	All 7 EPS samples were uncharged.
28318581	12	34	theme	low	1981:1983	arg1	levels					1985:1990	low levels	1981:1990	low levels of rhamnose in the EPS isolated from DGCC 7698	1981:2037	The monosaccharide compositions of the various EPS were mainly composed of glucose and galactose, with low levels of rhamnose in the EPS isolated from DGCC 7698, and N-acetylgalactosamine in the EPS from DGCC 7785, ST-10255y, and ST4239.
28318581	9	35	theme	EPS	1721:1723	arg1	values					1711:1716	The weight-average molar mass values	1681:1716	The weight-average molar mass values of EPS	1681:1723	The weight-average molar mass values of EPS ranged from 0.14 to 1.61 × 106 g/mol.
28318581	13	36	theme	EPS	2130:2132	arg1	yields					2120:2125	The yields	2116:2125	The yields of EPS (measured when fermented milks reached pH 4.6)	2116:2179	The yields of EPS (measured when fermented milks reached pH 4.6) ranged from 8.0 to 76.4 mg of glucose equivalents/kg.
28318581	9	37	theme	mass	1706:1709	arg1	values					1711:1716	The weight-average molar mass values	1681:1716	The weight-average molar mass values of EPS	1681:1723	The weight-average molar mass values of EPS ranged from 0.14 to 1.61 × 106 g/mol.
28318581	15	38	theme	Streptococcus	2530:2542	arg1	thermophilus					2544:2555	Streptococcus thermophilus	2530:2555	Streptococcus thermophilus	2530:2555	The results of our study will help the dairy industry to better understand the mechanism by which different strains of Streptococcus thermophilus affect yogurt texture.
28318581	2	39	theme	repeating	504:512	arg1	structure					519:527	repeating unit structure	504:527	repeating unit structure	504:527	The EPS from different species, or different strains from the same species, may differ in terms of molar mass, repeating unit structure, and EPS yield during fermentation of milk.
28318581	0	40	theme	various	102:108	arg1	strains					137:143	various Streptococcus thermophilus strains	102:143	various Streptococcus thermophilus strains	102:143	Characterization of the chemical structures and physical properties of exopolysaccharides produced by various Streptococcus thermophilus strains.
28318581	5	41	theme	properties	1008:1017	arg1	description					972:982	a complete description	961:982	a complete description of their EPS structural properties	961:1017	These strains were selected because they have been used in previous studies on yogurt texture, but a complete description of their EPS structural properties has not yet been reported.
28318581	7	42	theme	light	1475:1479	arg1	scattering					1481:1490	size-exclusion chromatography multiangle laser light scattering	1428:1490	size-exclusion chromatography multiangle laser light scattering	1428:1490	The EPS from each strain was isolated and the weight-average molar mass and z-average root mean square radius determined using size-exclusion chromatography multiangle laser light scattering.
28318581	2	43	theme	mass	498:501	arg1	terms					483:487	terms	483:487	terms of molar mass, repeating unit structure, and EPS yield	483:542	The EPS from different species, or different strains from the same species, may differ in terms of molar mass, repeating unit structure, and EPS yield during fermentation of milk.
28318581	11	44	contain	had	1830:1832	arg1	ST-10255y					1809:1817	ST-10255y	1809:1817	ST-10255y	1809:1817	The strains ST-10255y and ST4239 had EPS with the same repeating unit structure.
28318581	11	44	contain	had	1830:1832	arg2	EPS					1834:1836	EPS	1834:1836	EPS with the same repeating unit structure	1834:1875	The strains ST-10255y and ST4239 had EPS with the same repeating unit structure.
28318581	11	44	contain	had	1830:1832	arg1	strains					1801:1807	The strains ST-10255y and ST4239	1797:1828	The strains ST-10255y and ST4239	1797:1828	The strains ST-10255y and ST4239 had EPS with the same repeating unit structure.
28318581	11	44	contain	had	1830:1832	arg1	ST4239					1823:1828	ST4239	1823:1828	ST4239	1823:1828	The strains ST-10255y and ST4239 had EPS with the same repeating unit structure.
28318581	15	45	theme	thermophilus	2544:2555	arg1	strains					2519:2525	different strains	2509:2525	different strains of Streptococcus thermophilus	2509:2555	The results of our study will help the dairy industry to better understand the mechanism by which different strains of Streptococcus thermophilus affect yogurt texture.
28318581	12	46	attach	isolated	2015:2022	arg2	EPS					2011:2013	the EPS	2007:2013	the EPS isolated from DGCC 7698	2007:2037	The monosaccharide compositions of the various EPS were mainly composed of glucose and galactose, with low levels of rhamnose in the EPS isolated from DGCC 7698, and N-acetylgalactosamine in the EPS from DGCC 7785, ST-10255y, and ST4239.
28318581	12	46	attach	isolated	2015:2022	arg1	DGCC					2029:2032	DGCC 7698	2029:2037	DGCC 7698	2029:2037	The monosaccharide compositions of the various EPS were mainly composed of glucose and galactose, with low levels of rhamnose in the EPS isolated from DGCC 7698, and N-acetylgalactosamine in the EPS from DGCC 7785, ST-10255y, and ST4239.
28318581	14	47	theme	staining	2391:2398	arg1	technique					2400:2408	negative staining technique	2382:2408	negative staining technique	2382:2408	In addition to (free) EPS, some strains were also able to produce capsular polysaccharide (associated with the bacterial cells) when observed with negative staining technique.
28318581	11	48	theme	unit	1862:1865	arg1	structure					1867:1875	the same repeating unit structure	1843:1875	the same repeating unit structure	1843:1875	The strains ST-10255y and ST4239 had EPS with the same repeating unit structure.
28318581	1	49	theme	yogurt	300:305	arg1	properties					286:295	the rheological and physical properties	257:295	the rheological and physical properties of yogurt	257:305	Exopolysaccharides (EPS) produced by some lactic acid bacteria are often used by the dairy industry to improve the rheological and physical properties of yogurt, but the relationship between their structure and functional effect is still unclear.
28318581	7	50	theme	chromatography	1443:1456	arg1	scattering					1481:1490	size-exclusion chromatography multiangle laser light scattering	1428:1490	size-exclusion chromatography multiangle laser light scattering	1428:1490	The EPS from each strain was isolated and the weight-average molar mass and z-average root mean square radius determined using size-exclusion chromatography multiangle laser light scattering.
28318581	8	51	theme	magnetic	1648:1655	arg1	resonance					1657:1665	nuclear magnetic resonance	1640:1665	nuclear magnetic resonance spectroscopy	1640:1678	The monosaccharide composition of EPS was determined using gas chromatography-mass spectrometry, and repeating unit structure was determined using nuclear magnetic resonance spectroscopy.
28318581	11	52	theme	same	1847:1850	arg1	structure					1867:1875	the same repeating unit structure	1843:1875	the same repeating unit structure	1843:1875	The strains ST-10255y and ST4239 had EPS with the same repeating unit structure.
28318581	0	53	theme	structures	33:42	arg1	Characterization					0:15	Characterization	0:15	Characterization of the chemical structures and physical properties of exopolysaccharides	0:88	Characterization of the chemical structures and physical properties of exopolysaccharides produced by various Streptococcus thermophilus strains.
28318581	14	54	theme	negative	2382:2389	arg1	technique					2400:2408	negative staining technique	2382:2408	negative staining technique	2382:2408	In addition to (free) EPS, some strains were also able to produce capsular polysaccharide (associated with the bacterial cells) when observed with negative staining technique.
28318581	5	55	theme	yogurt	941:946	arg1	texture					948:954	yogurt texture	941:954	yogurt texture	941:954	These strains were selected because they have been used in previous studies on yogurt texture, but a complete description of their EPS structural properties has not yet been reported.
28318581	3	56	theme	detailed	610:617	arg1	properties					619:628	the detailed properties	606:628	the detailed properties of EPS produced from 7 strains of Streptococcus thermophilus	606:689	This study aimed to characterize the detailed properties of EPS produced from 7 strains of Streptococcus thermophilus, which is one of the key cultures used for yogurt manufacture.
28318581	13	57	dep	76.4	2200:2203	arg1	to					2197:2198	to	2197:2198	to	2197:2198	The yields of EPS (measured when fermented milks reached pH 4.6) ranged from 8.0 to 76.4 mg of glucose equivalents/kg.
28318581	12	58	with	N-acetylgalactosamine	2044:2064	arg1	levels					1985:1990	low levels	1981:1990	low levels of rhamnose in the EPS isolated from DGCC 7698	1981:2037	The monosaccharide compositions of the various EPS were mainly composed of glucose and galactose, with low levels of rhamnose in the EPS isolated from DGCC 7698, and N-acetylgalactosamine in the EPS from DGCC 7785, ST-10255y, and ST4239.
28318581	0	59	theme	physical	48:55	arg1	properties					57:66	physical properties	48:66	physical properties	48:66	Characterization of the chemical structures and physical properties of exopolysaccharides produced by various Streptococcus thermophilus strains.
28318581	0	60	dep	structures	33:42	arg1	the					20:22	the	20:22	the	20:22	Characterization of the chemical structures and physical properties of exopolysaccharides produced by various Streptococcus thermophilus strains.
28318581	12	61	from	ST-10255y	2093:2101	arg1	N-acetylgalactosamine					2044:2064	N-acetylgalactosamine	2044:2064	N-acetylgalactosamine	2044:2064	The monosaccharide compositions of the various EPS were mainly composed of glucose and galactose, with low levels of rhamnose in the EPS isolated from DGCC 7698, and N-acetylgalactosamine in the EPS from DGCC 7785, ST-10255y, and ST4239.
28318581	12	61	from	ST-10255y	2093:2101	arg1	galactose					1965:1973	galactose	1965:1973	galactose	1965:1973	The monosaccharide compositions of the various EPS were mainly composed of glucose and galactose, with low levels of rhamnose in the EPS isolated from DGCC 7698, and N-acetylgalactosamine in the EPS from DGCC 7785, ST-10255y, and ST4239.
28318581	12	61	from	ST-10255y	2093:2101	arg1	EPS					2073:2075	the EPS	2069:2075	the EPS from DGCC 7785, ST-10255y, and ST4239	2069:2113	The monosaccharide compositions of the various EPS were mainly composed of glucose and galactose, with low levels of rhamnose in the EPS isolated from DGCC 7698, and N-acetylgalactosamine in the EPS from DGCC 7785, ST-10255y, and ST4239.
28318581	12	61	from	ST-10255y	2093:2101	arg1	glucose					1953:1959	glucose	1953:1959	glucose	1953:1959	The monosaccharide compositions of the various EPS were mainly composed of glucose and galactose, with low levels of rhamnose in the EPS isolated from DGCC 7698, and N-acetylgalactosamine in the EPS from DGCC 7785, ST-10255y, and ST4239.
28318581	1	62	theme	dairy	231:235	arg1	industry					237:244	the dairy industry	227:244	the dairy industry	227:244	Exopolysaccharides (EPS) produced by some lactic acid bacteria are often used by the dairy industry to improve the rheological and physical properties of yogurt, but the relationship between their structure and functional effect is still unclear.
28318581	7	63	theme	mean	1392:1395	arg1	square					1397:1402	z-average root mean square	1377:1402	z-average root mean square	1377:1402	The EPS from each strain was isolated and the weight-average molar mass and z-average root mean square radius determined using size-exclusion chromatography multiangle laser light scattering.
28318581	9	64	theme	1.61	1745:1748	arg1	×					1750:1750	×	1750:1750	×	1750:1750	The weight-average molar mass values of EPS ranged from 0.14 to 1.61 × 106 g/mol.
28318581	2	65	from	species	416:422	arg1	EPS					397:399	The EPS	393:399	The EPS from different species	393:422	The EPS from different species, or different strains from the same species, may differ in terms of molar mass, repeating unit structure, and EPS yield during fermentation of milk.
28318581	2	65	from	species	416:422	arg1	strains					438:444	different strains	428:444	different strains from the same species	428:466	The EPS from different species, or different strains from the same species, may differ in terms of molar mass, repeating unit structure, and EPS yield during fermentation of milk.
28318581	8	66	theme	EPS	1527:1529	arg1	composition					1512:1522	The monosaccharide composition	1493:1522	The monosaccharide composition of EPS	1493:1529	The monosaccharide composition of EPS was determined using gas chromatography-mass spectrometry, and repeating unit structure was determined using nuclear magnetic resonance spectroscopy.
28318581	11	67	theme	repeating	1852:1860	arg1	structure					1867:1875	the same repeating unit structure	1843:1875	the same repeating unit structure	1843:1875	The strains ST-10255y and ST4239 had EPS with the same repeating unit structure.
28318581	8	68	theme	unit	1604:1607	arg1	structure					1609:1617	repeating unit structure	1594:1617	repeating unit structure	1594:1617	The monosaccharide composition of EPS was determined using gas chromatography-mass spectrometry, and repeating unit structure was determined using nuclear magnetic resonance spectroscopy.
28318581	15	69	theme	yogurt	2564:2569	arg1	texture					2571:2577	yogurt texture	2564:2577	yogurt texture	2564:2577	The results of our study will help the dairy industry to better understand the mechanism by which different strains of Streptococcus thermophilus affect yogurt texture.
28318581	7	70	theme	z-average	1377:1385	arg1	square					1397:1402	z-average root mean square	1377:1402	z-average root mean square	1377:1402	The EPS from each strain was isolated and the weight-average molar mass and z-average root mean square radius determined using size-exclusion chromatography multiangle laser light scattering.
28318581	3	71	theme	thermophilus	678:689	arg1	strains					653:659	7 strains	651:659	7 strains of Streptococcus thermophilus	651:689	This study aimed to characterize the detailed properties of EPS produced from 7 strains of Streptococcus thermophilus, which is one of the key cultures used for yogurt manufacture.
28318581	7	72	theme	mass	1368:1371	arg1	radius					1404:1409	the weight-average molar mass and z-average root mean square radius	1343:1409	the weight-average molar mass and z-average root mean square radius	1343:1409	The EPS from each strain was isolated and the weight-average molar mass and z-average root mean square radius determined using size-exclusion chromatography multiangle laser light scattering.
28318581	4	73	theme	DGCC	808:811	arg1	7785					813:816	DGCC 7785	808:816	DGCC 7785	808:816	Milk was fermented with strains DGCC 7698, DGCC 7710, DGCC 7785, ST-10255y, St-143, STCth-9204, and ST4239.
28318581	8	74	theme	gas	1552:1554	arg1	spectrometry					1576:1587	gas chromatography-mass spectrometry	1552:1587	gas chromatography-mass spectrometry	1552:1587	The monosaccharide composition of EPS was determined using gas chromatography-mass spectrometry, and repeating unit structure was determined using nuclear magnetic resonance spectroscopy.
28318581	9	75	dep	×	1750:1750	arg1	to					1742:1743	to	1742:1743	to	1742:1743	The weight-average molar mass values of EPS ranged from 0.14 to 1.61 × 106 g/mol.
28318581	14	76	theme	free	2251:2254	arg1	EPS					2257:2259	(free) EPS	2250:2259	(free) EPS	2250:2259	In addition to (free) EPS, some strains were also able to produce capsular polysaccharide (associated with the bacterial cells) when observed with negative staining technique.
28318581	7	77	theme	weight-average	1347:1360	arg1	mass					1368:1371	the weight-average molar mass	1343:1371	the weight-average molar mass	1343:1371	The EPS from each strain was isolated and the weight-average molar mass and z-average root mean square radius determined using size-exclusion chromatography multiangle laser light scattering.
28318581	6	78	theme	acidification	1089:1101	arg1	rate					1103:1106	a similar acidification rate	1079:1106	a similar acidification rate	1079:1106	All strains were fermented under a similar acidification rate by adjusting the level of supplementation with peptone or the inoculation level, which allowed for a comparison of EPS yields under similar growth conditions (reconstituted skim milk at 40°C).
28318581	5	79	from	studies	930:936	arg1	texture					948:954	yogurt texture	941:954	yogurt texture	941:954	These strains were selected because they have been used in previous studies on yogurt texture, but a complete description of their EPS structural properties has not yet been reported.
28318581	1	80	theme	lactic	188:193	arg1	bacteria					200:207	some lactic acid bacteria	183:207	some lactic acid bacteria	183:207	Exopolysaccharides (EPS) produced by some lactic acid bacteria are often used by the dairy industry to improve the rheological and physical properties of yogurt, but the relationship between their structure and functional effect is still unclear.
28318581	6	81	dep	allowed	1195:1201	arg1	milk					1286:1289	reconstituted skim milk	1267:1289	reconstituted skim milk at 40°C	1267:1297	All strains were fermented under a similar acidification rate by adjusting the level of supplementation with peptone or the inoculation level, which allowed for a comparison of EPS yields under similar growth conditions (reconstituted skim milk at 40°C).
28318581	14	82	theme	capsular	2301:2308	arg1	polysaccharide					2310:2323	capsular polysaccharide	2301:2323	capsular polysaccharide (associated with the bacterial cells)	2301:2361	In addition to (free) EPS, some strains were also able to produce capsular polysaccharide (associated with the bacterial cells) when observed with negative staining technique.
28318581	3	83	theme	cultures	716:723	arg1	cultures					716:723	the key cultures	708:723	the key cultures used for yogurt manufacture	708:751	This study aimed to characterize the detailed properties of EPS produced from 7 strains of Streptococcus thermophilus, which is one of the key cultures used for yogurt manufacture.
28318581	3	83	theme	cultures	716:723	arg1	one					701:703	one	701:703	one	701:703	This study aimed to characterize the detailed properties of EPS produced from 7 strains of Streptococcus thermophilus, which is one of the key cultures used for yogurt manufacture.
28318581	6	84	theme	growth	1248:1253	arg1	conditions					1255:1264	similar growth conditions	1240:1264	similar growth conditions	1240:1264	All strains were fermented under a similar acidification rate by adjusting the level of supplementation with peptone or the inoculation level, which allowed for a comparison of EPS yields under similar growth conditions (reconstituted skim milk at 40°C).
28318581	12	85	theme	various	1917:1923	arg1	EPS					1925:1927	the various EPS	1913:1927	the various EPS	1913:1927	The monosaccharide compositions of the various EPS were mainly composed of glucose and galactose, with low levels of rhamnose in the EPS isolated from DGCC 7698, and N-acetylgalactosamine in the EPS from DGCC 7785, ST-10255y, and ST4239.
28318581	12	86	with	galactose	1965:1973	arg1	levels					1985:1990	low levels	1981:1990	low levels of rhamnose in the EPS isolated from DGCC 7698	1981:2037	The monosaccharide compositions of the various EPS were mainly composed of glucose and galactose, with low levels of rhamnose in the EPS isolated from DGCC 7698, and N-acetylgalactosamine in the EPS from DGCC 7785, ST-10255y, and ST4239.
28318581	15	87	theme	dairy	2450:2454	arg1	industry					2456:2463	the dairy industry	2446:2463	the dairy industry	2446:2463	The results of our study will help the dairy industry to better understand the mechanism by which different strains of Streptococcus thermophilus affect yogurt texture.
28318581	4	88	dep	strains	778:784	arg1	ST4239					854:859	ST4239	854:859	ST4239	854:859	Milk was fermented with strains DGCC 7698, DGCC 7710, DGCC 7785, ST-10255y, St-143, STCth-9204, and ST4239.
28318581	4	88	dep	strains	778:784	arg1	7710					802:805	DGCC 7710	797:805	DGCC 7710	797:805	Milk was fermented with strains DGCC 7698, DGCC 7710, DGCC 7785, ST-10255y, St-143, STCth-9204, and ST4239.
28318581	4	88	dep	strains	778:784	arg1	St-143					830:835	St-143	830:835	St-143	830:835	Milk was fermented with strains DGCC 7698, DGCC 7710, DGCC 7785, ST-10255y, St-143, STCth-9204, and ST4239.
28318581	4	88	dep	strains	778:784	arg1	STCth-9204					838:847	STCth-9204	838:847	STCth-9204	838:847	Milk was fermented with strains DGCC 7698, DGCC 7710, DGCC 7785, ST-10255y, St-143, STCth-9204, and ST4239.
28318581	4	88	dep	strains	778:784	arg1	7698					791:794	DGCC 7698	786:794	DGCC 7698	786:794	Milk was fermented with strains DGCC 7698, DGCC 7710, DGCC 7785, ST-10255y, St-143, STCth-9204, and ST4239.
28318581	4	88	dep	strains	778:784	arg1	7785					813:816	DGCC 7785	808:816	DGCC 7785	808:816	Milk was fermented with strains DGCC 7698, DGCC 7710, DGCC 7785, ST-10255y, St-143, STCth-9204, and ST4239.
28318581	4	88	dep	strains	778:784	arg1	ST-10255y					819:827	ST-10255y	819:827	ST-10255y	819:827	Milk was fermented with strains DGCC 7698, DGCC 7710, DGCC 7785, ST-10255y, St-143, STCth-9204, and ST4239.
28318581	2	89	theme	unit	514:517	arg1	structure					519:527	repeating unit structure	504:527	repeating unit structure	504:527	The EPS from different species, or different strains from the same species, may differ in terms of molar mass, repeating unit structure, and EPS yield during fermentation of milk.
28318581	0	90	theme	Streptococcus	110:122	arg1	strains					137:143	various Streptococcus thermophilus strains	102:143	various Streptococcus thermophilus strains	102:143	Characterization of the chemical structures and physical properties of exopolysaccharides produced by various Streptococcus thermophilus strains.
28318581	6	91	theme	EPS	1223:1225	arg1	yields					1227:1232	EPS yields	1223:1232	EPS yields	1223:1232	All strains were fermented under a similar acidification rate by adjusting the level of supplementation with peptone or the inoculation level, which allowed for a comparison of EPS yields under similar growth conditions (reconstituted skim milk at 40°C).
28318581	5	92	theme	structural	997:1006	arg1	properties					1008:1017	their EPS structural properties	987:1017	their EPS structural properties	987:1017	These strains were selected because they have been used in previous studies on yogurt texture, but a complete description of their EPS structural properties has not yet been reported.
28318581	6	93	from	40°C	1294:1297	arg1	milk					1286:1289	reconstituted skim milk	1267:1289	reconstituted skim milk at 40°C	1267:1297	All strains were fermented under a similar acidification rate by adjusting the level of supplementation with peptone or the inoculation level, which allowed for a comparison of EPS yields under similar growth conditions (reconstituted skim milk at 40°C).
28318581	9	94	theme	weight-average	1685:1698	arg1	values					1711:1716	The weight-average molar mass values	1681:1716	The weight-average molar mass values of EPS	1681:1723	The weight-average molar mass values of EPS ranged from 0.14 to 1.61 × 106 g/mol.
28318581	12	95	theme	rhamnose	1995:2002	arg1	levels					1985:1990	low levels	1981:1990	low levels of rhamnose in the EPS isolated from DGCC 7698	1981:2037	The monosaccharide compositions of the various EPS were mainly composed of glucose and galactose, with low levels of rhamnose in the EPS isolated from DGCC 7698, and N-acetylgalactosamine in the EPS from DGCC 7785, ST-10255y, and ST4239.
28318581	6	96	with	supplementation	1134:1148	arg1	peptone					1155:1161	peptone	1155:1161	peptone	1155:1161	All strains were fermented under a similar acidification rate by adjusting the level of supplementation with peptone or the inoculation level, which allowed for a comparison of EPS yields under similar growth conditions (reconstituted skim milk at 40°C).
28318581	2	97	theme	EPS	534:536	arg1	yield					538:542	EPS yield	534:542	EPS yield	534:542	The EPS from different species, or different strains from the same species, may differ in terms of molar mass, repeating unit structure, and EPS yield during fermentation of milk.
28318581	0	98	theme	exopolysaccharides	71:88	arg1	structures					33:42	chemical structures	24:42	chemical structures	24:42	Characterization of the chemical structures and physical properties of exopolysaccharides produced by various Streptococcus thermophilus strains.
28318581	0	98	theme	exopolysaccharides	71:88	arg1	properties					57:66	physical properties	48:66	physical properties	48:66	Characterization of the chemical structures and physical properties of exopolysaccharides produced by various Streptococcus thermophilus strains.
28318581	12	99	from	levels	1985:1990	arg1	EPS					2011:2013	the EPS	2007:2013	the EPS isolated from DGCC 7698	2007:2037	The monosaccharide compositions of the various EPS were mainly composed of glucose and galactose, with low levels of rhamnose in the EPS isolated from DGCC 7698, and N-acetylgalactosamine in the EPS from DGCC 7785, ST-10255y, and ST4239.
28318581	6	100	theme	reconstituted	1267:1279	arg1	milk					1286:1289	reconstituted skim milk	1267:1289	reconstituted skim milk at 40°C	1267:1297	All strains were fermented under a similar acidification rate by adjusting the level of supplementation with peptone or the inoculation level, which allowed for a comparison of EPS yields under similar growth conditions (reconstituted skim milk at 40°C).
28318581	13	101	theme	fermented	2149:2157	arg1	milks					2159:2163	fermented milks	2149:2163	fermented milks	2149:2163	The yields of EPS (measured when fermented milks reached pH 4.6) ranged from 8.0 to 76.4 mg of glucose equivalents/kg.
28318581	7	102	from	strain	1319:1324	arg1	EPS					1305:1307	The EPS	1301:1307	The EPS from each strain	1301:1324	The EPS from each strain was isolated and the weight-average molar mass and z-average root mean square radius determined using size-exclusion chromatography multiangle laser light scattering.
28318581	6	103	theme	skim	1281:1284	arg1	milk					1286:1289	reconstituted skim milk	1267:1289	reconstituted skim milk at 40°C	1267:1297	All strains were fermented under a similar acidification rate by adjusting the level of supplementation with peptone or the inoculation level, which allowed for a comparison of EPS yields under similar growth conditions (reconstituted skim milk at 40°C).
28318581	1	104	theme	functional	357:366	arg1	effect					368:373	functional effect	357:373	functional effect	357:373	Exopolysaccharides (EPS) produced by some lactic acid bacteria are often used by the dairy industry to improve the rheological and physical properties of yogurt, but the relationship between their structure and functional effect is still unclear.
28318581	12	105	from	N-acetylgalactosamine	2044:2064	arg1	DGCC					2082:2085	DGCC 7785	2082:2090	DGCC 7785	2082:2090	The monosaccharide compositions of the various EPS were mainly composed of glucose and galactose, with low levels of rhamnose in the EPS isolated from DGCC 7698, and N-acetylgalactosamine in the EPS from DGCC 7785, ST-10255y, and ST4239.
28318581	12	105	from	N-acetylgalactosamine	2044:2064	arg1	ST-10255y					2093:2101	ST-10255y	2093:2101	ST-10255y	2093:2101	The monosaccharide compositions of the various EPS were mainly composed of glucose and galactose, with low levels of rhamnose in the EPS isolated from DGCC 7698, and N-acetylgalactosamine in the EPS from DGCC 7785, ST-10255y, and ST4239.
28318581	12	105	from	N-acetylgalactosamine	2044:2064	arg1	EPS					2073:2075	the EPS	2069:2075	the EPS from DGCC 7785, ST-10255y, and ST4239	2069:2113	The monosaccharide compositions of the various EPS were mainly composed of glucose and galactose, with low levels of rhamnose in the EPS isolated from DGCC 7698, and N-acetylgalactosamine in the EPS from DGCC 7785, ST-10255y, and ST4239.
28318581	12	105	from	N-acetylgalactosamine	2044:2064	arg1	ST4239					2108:2113	ST4239	2108:2113	ST4239	2108:2113	The monosaccharide compositions of the various EPS were mainly composed of glucose and galactose, with low levels of rhamnose in the EPS isolated from DGCC 7698, and N-acetylgalactosamine in the EPS from DGCC 7785, ST-10255y, and ST4239.
28318581	7	106	theme	laser	1469:1473	arg1	scattering					1481:1490	size-exclusion chromatography multiangle laser light scattering	1428:1490	size-exclusion chromatography multiangle laser light scattering	1428:1490	The EPS from each strain was isolated and the weight-average molar mass and z-average root mean square radius determined using size-exclusion chromatography multiangle laser light scattering.
28318581	9	107	theme	0.14	1737:1740	arg1	×					1750:1750	×	1750:1750	×	1750:1750	The weight-average molar mass values of EPS ranged from 0.14 to 1.61 × 106 g/mol.
28318581	2	108	from	species	460:466	arg1	EPS					397:399	The EPS	393:399	The EPS from different species	393:422	The EPS from different species, or different strains from the same species, may differ in terms of molar mass, repeating unit structure, and EPS yield during fermentation of milk.
28318581	2	108	from	species	460:466	arg1	strains					438:444	different strains	428:444	different strains from the same species	428:466	The EPS from different species, or different strains from the same species, may differ in terms of molar mass, repeating unit structure, and EPS yield during fermentation of milk.
28318581	0	109	dep	Streptococcus	110:122	arg1	thermophilus					124:135	thermophilus	124:135	thermophilus	124:135	Characterization of the chemical structures and physical properties of exopolysaccharides produced by various Streptococcus thermophilus strains.
28318581	2	110	theme	molar	492:496	arg1	mass					498:501	molar mass	492:501	molar mass	492:501	The EPS from different species, or different strains from the same species, may differ in terms of molar mass, repeating unit structure, and EPS yield during fermentation of milk.
28318581	11	111	dep	strains	1801:1807	arg1	ST-10255y					1809:1817	ST-10255y	1809:1817	ST-10255y	1809:1817	The strains ST-10255y and ST4239 had EPS with the same repeating unit structure.
28318581	11	111	dep	strains	1801:1807	arg1	strains					1801:1807	The strains ST-10255y and ST4239	1797:1828	The strains ST-10255y and ST4239	1797:1828	The strains ST-10255y and ST4239 had EPS with the same repeating unit structure.
28318581	11	111	dep	strains	1801:1807	arg1	ST4239					1823:1828	ST4239	1823:1828	ST4239	1823:1828	The strains ST-10255y and ST4239 had EPS with the same repeating unit structure.
28318581	9	112	theme	106	1752:1754	arg1	×					1750:1750	×	1750:1750	×	1750:1750	The weight-average molar mass values of EPS ranged from 0.14 to 1.61 × 106 g/mol.
28318581	0	113	theme	chemical	24:31	arg1	structures					33:42	chemical structures	24:42	chemical structures	24:42	Characterization of the chemical structures and physical properties of exopolysaccharides produced by various Streptococcus thermophilus strains.
28318581	2	114	theme	different	428:436	arg1	strains					438:444	different strains	428:444	different strains from the same species	428:466	The EPS from different species, or different strains from the same species, may differ in terms of molar mass, repeating unit structure, and EPS yield during fermentation of milk.
28318581	7	115	theme	multiangle	1458:1467	arg1	scattering					1481:1490	size-exclusion chromatography multiangle laser light scattering	1428:1490	size-exclusion chromatography multiangle laser light scattering	1428:1490	The EPS from each strain was isolated and the weight-average molar mass and z-average root mean square radius determined using size-exclusion chromatography multiangle laser light scattering.
28318581	15	116	theme	study	2430:2434	arg1	results					2415:2421	The results	2411:2421	The results of our study	2411:2434	The results of our study will help the dairy industry to better understand the mechanism by which different strains of Streptococcus thermophilus affect yogurt texture.
28318581	8	117	theme	monosaccharide	1497:1510	arg1	composition					1512:1522	The monosaccharide composition	1493:1522	The monosaccharide composition of EPS	1493:1529	The monosaccharide composition of EPS was determined using gas chromatography-mass spectrometry, and repeating unit structure was determined using nuclear magnetic resonance spectroscopy.
28318581	2	118	theme	different	406:414	arg1	species					416:422	different species	406:422	different species	406:422	The EPS from different species, or different strains from the same species, may differ in terms of molar mass, repeating unit structure, and EPS yield during fermentation of milk.
28318581	0	119	theme	properties	57:66	arg1	Characterization					0:15	Characterization	0:15	Characterization of the chemical structures and physical properties of exopolysaccharides	0:88	Characterization of the chemical structures and physical properties of exopolysaccharides produced by various Streptococcus thermophilus strains.
28318581	7	120	theme	size-exclusion	1428:1441	arg1	chromatography					1443:1456	size-exclusion chromatography	1428:1456	size-exclusion chromatography multiangle laser light scattering	1428:1490	The EPS from each strain was isolated and the weight-average molar mass and z-average root mean square radius determined using size-exclusion chromatography multiangle laser light scattering.
28318581	2	121	theme	same	455:458	arg1	species					460:466	the same species	451:466	the same species	451:466	The EPS from different species, or different strains from the same species, may differ in terms of molar mass, repeating unit structure, and EPS yield during fermentation of milk.
28318581	14	122	theme	bacterial	2346:2354	arg1	cells					2356:2360	the bacterial cells	2342:2360	the bacterial cells	2342:2360	In addition to (free) EPS, some strains were also able to produce capsular polysaccharide (associated with the bacterial cells) when observed with negative staining technique.
28318581	4	123	theme	DGCC	786:789	arg1	7698					791:794	DGCC 7698	786:794	DGCC 7698	786:794	Milk was fermented with strains DGCC 7698, DGCC 7710, DGCC 7785, ST-10255y, St-143, STCth-9204, and ST4239.
28318581	8	124	theme	repeating	1594:1602	arg1	structure					1609:1617	repeating unit structure	1594:1617	repeating unit structure	1594:1617	The monosaccharide composition of EPS was determined using gas chromatography-mass spectrometry, and repeating unit structure was determined using nuclear magnetic resonance spectroscopy.
28318581	13	125	theme	equivalents/kg	2219:2232	arg1	mg					2205:2206	8.0 to 76.4 mg	2193:2206	8.0 to 76.4 mg of glucose equivalents/kg	2193:2232	The yields of EPS (measured when fermented milks reached pH 4.6) ranged from 8.0 to 76.4 mg of glucose equivalents/kg.
28318581	3	126	theme	EPS	633:635	arg1	properties					619:628	the detailed properties	606:628	the detailed properties of EPS produced from 7 strains of Streptococcus thermophilus	606:689	This study aimed to characterize the detailed properties of EPS produced from 7 strains of Streptococcus thermophilus, which is one of the key cultures used for yogurt manufacture.
28318581	5	127	used	used	913:916	arg2	they					898:901	they	898:901	they	898:901	These strains were selected because they have been used in previous studies on yogurt texture, but a complete description of their EPS structural properties has not yet been reported.
28318581	7	128	theme	square	1397:1402	arg1	radius					1404:1409	the weight-average molar mass and z-average root mean square radius	1343:1409	the weight-average molar mass and z-average root mean square radius	1343:1409	The EPS from each strain was isolated and the weight-average molar mass and z-average root mean square radius determined using size-exclusion chromatography multiangle laser light scattering.
28318581	5	129	theme	previous	921:928	arg1	studies					930:936	previous studies	921:936	previous studies on yogurt texture	921:954	These strains were selected because they have been used in previous studies on yogurt texture, but a complete description of their EPS structural properties has not yet been reported.
28318581	12	130	from	DGCC	2082:2085	arg1	N-acetylgalactosamine					2044:2064	N-acetylgalactosamine	2044:2064	N-acetylgalactosamine	2044:2064	The monosaccharide compositions of the various EPS were mainly composed of glucose and galactose, with low levels of rhamnose in the EPS isolated from DGCC 7698, and N-acetylgalactosamine in the EPS from DGCC 7785, ST-10255y, and ST4239.
28318581	12	130	from	DGCC	2082:2085	arg1	galactose					1965:1973	galactose	1965:1973	galactose	1965:1973	The monosaccharide compositions of the various EPS were mainly composed of glucose and galactose, with low levels of rhamnose in the EPS isolated from DGCC 7698, and N-acetylgalactosamine in the EPS from DGCC 7785, ST-10255y, and ST4239.
28318581	12	130	from	DGCC	2082:2085	arg1	EPS					2073:2075	the EPS	2069:2075	the EPS from DGCC 7785, ST-10255y, and ST4239	2069:2113	The monosaccharide compositions of the various EPS were mainly composed of glucose and galactose, with low levels of rhamnose in the EPS isolated from DGCC 7698, and N-acetylgalactosamine in the EPS from DGCC 7785, ST-10255y, and ST4239.
28318581	12	130	from	DGCC	2082:2085	arg1	glucose					1953:1959	glucose	1953:1959	glucose	1953:1959	The monosaccharide compositions of the various EPS were mainly composed of glucose and galactose, with low levels of rhamnose in the EPS isolated from DGCC 7698, and N-acetylgalactosamine in the EPS from DGCC 7785, ST-10255y, and ST4239.
27515282	4	0	theme	glycanated	882:891	arg1	biglycan					893:900	glycanated biglycan	882:900	glycanated biglycan	882:900	In separate experiments, we have shown that similarly to glycanated biglycan, recombinant human biglycan core protein (rhBGNc) protects cardiomyocytes against SI/R injury.
27515282	8	1	theme	ERK1/2	1721:1726	arg1	phosphorylation					1702:1716	phosphorylation	1702:1716	phosphorylation of ERK1/2, STAT3	1702:1733	Pretreatment of cardiomyocytes with rhBGNc for 20h resulted in increased Akt phosphorylation and NO production without having significant effect on phosphorylation of ERK1/2, STAT3, and on the production of superoxide.
27515282	1	2	theme	ischemia/reperfusion	284:303	arg1	SI/R					313:316	SI/R	313:316	SI/R	313:316	AIMS Exogenously administered biglycan (core protein with high-molecular weight glycosaminoglycan chains) has been shown to protect neonatal cardiomyocytes against simulated ischemia/reperfusion injury (SI/R), however, the mechanism of action is not clear.
27515282	1	2	theme	ischemia/reperfusion	284:303	arg1	injury					305:310	simulated ischemia/reperfusion injury	274:310	simulated ischemia/reperfusion injury (SI/R)	274:317	AIMS Exogenously administered biglycan (core protein with high-molecular weight glycosaminoglycan chains) has been shown to protect neonatal cardiomyocytes against simulated ischemia/reperfusion injury (SI/R), however, the mechanism of action is not clear.
27515282	11	3	theme	ERK	2158:2160	arg1	production					2204:2213	increased NO production	2191:2213	increased NO production	2191:2213	CONCLUSIONS The core protein of exogenous biglycan protects myocardial cells from SI/R injury via TLR4-mediated mechanisms involving activation of ERK, JNK and p38 MAP kinases and increased NO production.
27515282	11	3	theme	ERK	2158:2160	arg1	activation					2144:2153	activation	2144:2153	activation of ERK, JNK and p38 MAP kinases	2144:2185	CONCLUSIONS The core protein of exogenous biglycan protects myocardial cells from SI/R injury via TLR4-mediated mechanisms involving activation of ERK, JNK and p38 MAP kinases and increased NO production.
27515282	3	4	theme	pilot	598:602	arg1	study					604:608	A pilot study	596:608	A pilot study	596:608	METHODS AND RESULTS A pilot study was conducted to demonstrate that both native (glycanated) and deglycanated biglycan can attenuate cell death induced by SI/R in a dose-dependent manner in primary neonatal cardiomyocytes isolated from Wistar rats.
27515282	6	5	theme	potential	1303:1311	arg1	markers					1248:1254	markers	1248:1254	markers of necrosis, apoptosis, mitochondrial membrane potential, and autophagy	1248:1326	Treatment of cardiomyocytes with rhBGNc reverses the effect of SI/R upon markers of necrosis, apoptosis, mitochondrial membrane potential, and autophagy.
27515282	11	6	theme	biglycan	2053:2060	arg1	protein					2032:2038	The core protein	2023:2038	The core protein of exogenous biglycan protects myocardial cells from SI/R injury via TLR4-mediated mechanisms involving activation of ERK, JNK and p38 MAP kinases and increased NO production	2023:2213	CONCLUSIONS The core protein of exogenous biglycan protects myocardial cells from SI/R injury via TLR4-mediated mechanisms involving activation of ERK, JNK and p38 MAP kinases and increased NO production.
27515282	12	7	from	changes	2289:2295	arg1	autophagy					2324:2332	autophagy	2324:2332	autophagy	2324:2332	The cytoprotective effect of rhBGNc is due to modulation of SI/R-induced changes in necrosis, apoptosis and autophagy.
27515282	12	7	from	changes	2289:2295	arg1	apoptosis					2310:2318	apoptosis	2310:2318	apoptosis	2310:2318	The cytoprotective effect of rhBGNc is due to modulation of SI/R-induced changes in necrosis, apoptosis and autophagy.
27515282	12	7	from	changes	2289:2295	arg1	necrosis					2300:2307	necrosis	2300:2307	necrosis	2300:2307	The cytoprotective effect of rhBGNc is due to modulation of SI/R-induced changes in necrosis, apoptosis and autophagy.
27515282	6	8	theme	mitochondrial	1280:1292	arg1	potential					1303:1311	mitochondrial membrane potential	1280:1311	mitochondrial membrane potential	1280:1311	Treatment of cardiomyocytes with rhBGNc reverses the effect of SI/R upon markers of necrosis, apoptosis, mitochondrial membrane potential, and autophagy.
27515282	1	9	theme	glycosaminoglycan	190:206	arg1	chains					208:213	high-molecular weight glycosaminoglycan chains	168:213	high-molecular weight glycosaminoglycan chains	168:213	AIMS Exogenously administered biglycan (core protein with high-molecular weight glycosaminoglycan chains) has been shown to protect neonatal cardiomyocytes against simulated ischemia/reperfusion injury (SI/R), however, the mechanism of action is not clear.
27515282	11	10	theme	p38	2171:2173	arg1	production					2204:2213	increased NO production	2191:2213	increased NO production	2191:2213	CONCLUSIONS The core protein of exogenous biglycan protects myocardial cells from SI/R injury via TLR4-mediated mechanisms involving activation of ERK, JNK and p38 MAP kinases and increased NO production.
27515282	11	10	theme	p38	2171:2173	arg1	activation					2144:2153	activation	2144:2153	activation of ERK, JNK and p38 MAP kinases	2144:2185	CONCLUSIONS The core protein of exogenous biglycan protects myocardial cells from SI/R injury via TLR4-mediated mechanisms involving activation of ERK, JNK and p38 MAP kinases and increased NO production.
27515282	7	11	theme	downstream	1426:1435	arg1	mediators					1437:1445	its downstream mediators	1422:1445	its downstream mediators (IRAK1/4, ERK, JNK and p38 MAP kinases)	1422:1485	We have also shown that pharmacological blockade of Toll-like receptor 4 (TLR4) signaling or its downstream mediators (IRAK1/4, ERK, JNK and p38 MAP kinases) abolished the cytoprotective effect of rhBGNc against SI/R injury.
27515282	10	12	theme	rhBGNc	2003:2008	arg1	effect					1993:1998	the cardiocytoprotective effect	1968:1998	the cardiocytoprotective effect of rhBGNc	1968:2008	Blockade of NO synthesis also prevented the cardiocytoprotective effect of rhBGNc.
27515282	6	13	theme	apoptosis	1269:1277	arg1	markers					1248:1254	markers	1248:1254	markers of necrosis, apoptosis, mitochondrial membrane potential, and autophagy	1248:1326	Treatment of cardiomyocytes with rhBGNc reverses the effect of SI/R upon markers of necrosis, apoptosis, mitochondrial membrane potential, and autophagy.
27515282	9	14	from	increase	1868:1875	arg1	production					1891:1900	superoxide production	1880:1900	superoxide production	1880:1900	Treatment over 10min and 1h with rhBGNc increased ERK1 phosphorylation, while the SI/R-induced increase in superoxide production was attenuated by rhBGNc.
27515282	3	15	theme	dose-dependent	741:754	arg1	manner					756:761	a dose-dependent manner	739:761	a dose-dependent manner in primary neonatal cardiomyocytes isolated from Wistar rats	739:822	METHODS AND RESULTS A pilot study was conducted to demonstrate that both native (glycanated) and deglycanated biglycan can attenuate cell death induced by SI/R in a dose-dependent manner in primary neonatal cardiomyocytes isolated from Wistar rats.
27515282	11	16	theme	myocardial	2071:2080	arg1	cells					2082:2086	myocardial cells	2071:2086	myocardial cells	2071:2086	CONCLUSIONS The core protein of exogenous biglycan protects myocardial cells from SI/R injury via TLR4-mediated mechanisms involving activation of ERK, JNK and p38 MAP kinases and increased NO production.
27515282	1	17	theme	administered	127:138	arg1	biglycan					140:147	biglycan	140:147	AIMS Exogenously administered biglycan (core protein with high-molecular weight glycosaminoglycan chains)	110:214	AIMS Exogenously administered biglycan (core protein with high-molecular weight glycosaminoglycan chains) has been shown to protect neonatal cardiomyocytes against simulated ischemia/reperfusion injury (SI/R), however, the mechanism of action is not clear.
27515282	11	18	theme	SI/R	2093:2096	arg1	injury					2098:2103	SI/R injury	2093:2103	SI/R injury	2093:2103	CONCLUSIONS The core protein of exogenous biglycan protects myocardial cells from SI/R injury via TLR4-mediated mechanisms involving activation of ERK, JNK and p38 MAP kinases and increased NO production.
27515282	11	19	theme	increased	2191:2199	arg1	production					2204:2213	increased NO production	2191:2213	increased NO production	2191:2213	CONCLUSIONS The core protein of exogenous biglycan protects myocardial cells from SI/R injury via TLR4-mediated mechanisms involving activation of ERK, JNK and p38 MAP kinases and increased NO production.
27515282	9	20	theme	ERK1	1823:1826	arg1	phosphorylation					1828:1842	ERK1 phosphorylation	1823:1842	ERK1 phosphorylation	1823:1842	Treatment over 10min and 1h with rhBGNc increased ERK1 phosphorylation, while the SI/R-induced increase in superoxide production was attenuated by rhBGNc.
27515282	4	21	theme	human	915:919	arg1	rhBGNc					944:949	rhBGNc	944:949	rhBGNc	944:949	In separate experiments, we have shown that similarly to glycanated biglycan, recombinant human biglycan core protein (rhBGNc) protects cardiomyocytes against SI/R injury.
27515282	4	21	theme	human	915:919	arg1	protein					935:941	recombinant human biglycan core protein	903:941	recombinant human biglycan core protein (rhBGNc)	903:950	In separate experiments, we have shown that similarly to glycanated biglycan, recombinant human biglycan core protein (rhBGNc) protects cardiomyocytes against SI/R injury.
27515282	3	22	theme	neonatal	774:781	arg1	cardiomyocytes					783:796	primary neonatal cardiomyocytes	766:796	primary neonatal cardiomyocytes isolated from Wistar rats	766:822	METHODS AND RESULTS A pilot study was conducted to demonstrate that both native (glycanated) and deglycanated biglycan can attenuate cell death induced by SI/R in a dose-dependent manner in primary neonatal cardiomyocytes isolated from Wistar rats.
27515282	5	23	theme	significant	1066:1076	arg1	effect					1078:1083	no significant effect	1063:1083	no significant effect	1063:1083	In contrast, the glycosaminoglycan component dermatan sulfate had no significant effect on cell viability, while chondroitin sulfate further enhanced cell death induced by SI/R.
27515282	7	24	dep	IRAK1/4	1448:1454	arg1	p38					1470:1472	p38	1470:1472	p38	1470:1472	We have also shown that pharmacological blockade of Toll-like receptor 4 (TLR4) signaling or its downstream mediators (IRAK1/4, ERK, JNK and p38 MAP kinases) abolished the cytoprotective effect of rhBGNc against SI/R injury.
27515282	7	24	dep	IRAK1/4	1448:1454	arg1	JNK					1462:1464	JNK	1462:1464	JNK	1462:1464	We have also shown that pharmacological blockade of Toll-like receptor 4 (TLR4) signaling or its downstream mediators (IRAK1/4, ERK, JNK and p38 MAP kinases) abolished the cytoprotective effect of rhBGNc against SI/R injury.
27515282	7	24	dep	IRAK1/4	1448:1454	arg1	ERK					1457:1459	ERK	1457:1459	ERK	1457:1459	We have also shown that pharmacological blockade of Toll-like receptor 4 (TLR4) signaling or its downstream mediators (IRAK1/4, ERK, JNK and p38 MAP kinases) abolished the cytoprotective effect of rhBGNc against SI/R injury.
27515282	4	25	theme	core	930:933	arg1	rhBGNc					944:949	rhBGNc	944:949	rhBGNc	944:949	In separate experiments, we have shown that similarly to glycanated biglycan, recombinant human biglycan core protein (rhBGNc) protects cardiomyocytes against SI/R injury.
27515282	4	25	theme	core	930:933	arg1	protein					935:941	recombinant human biglycan core protein	903:941	recombinant human biglycan core protein (rhBGNc)	903:950	In separate experiments, we have shown that similarly to glycanated biglycan, recombinant human biglycan core protein (rhBGNc) protects cardiomyocytes against SI/R injury.
27515282	7	26	theme	receptor	1391:1398	arg1	signaling					1409:1417	Toll-like receptor 4 (TLR4) signaling	1381:1417	Toll-like receptor 4 (TLR4) signaling	1381:1417	We have also shown that pharmacological blockade of Toll-like receptor 4 (TLR4) signaling or its downstream mediators (IRAK1/4, ERK, JNK and p38 MAP kinases) abolished the cytoprotective effect of rhBGNc against SI/R injury.
27515282	11	27	theme	core	2027:2030	arg1	protein					2032:2038	The core protein	2023:2038	The core protein of exogenous biglycan protects myocardial cells from SI/R injury via TLR4-mediated mechanisms involving activation of ERK, JNK and p38 MAP kinases and increased NO production	2023:2213	CONCLUSIONS The core protein of exogenous biglycan protects myocardial cells from SI/R injury via TLR4-mediated mechanisms involving activation of ERK, JNK and p38 MAP kinases and increased NO production.
27515282	7	28	dep	ERK	1457:1459	arg1	kinases					1478:1484	MAP kinases	1474:1484	MAP kinases	1474:1484	We have also shown that pharmacological blockade of Toll-like receptor 4 (TLR4) signaling or its downstream mediators (IRAK1/4, ERK, JNK and p38 MAP kinases) abolished the cytoprotective effect of rhBGNc against SI/R injury.
27515282	3	29	theme	Wistar	812:817	arg1	rats					819:822	Wistar rats	812:822	Wistar rats	812:822	METHODS AND RESULTS A pilot study was conducted to demonstrate that both native (glycanated) and deglycanated biglycan can attenuate cell death induced by SI/R in a dose-dependent manner in primary neonatal cardiomyocytes isolated from Wistar rats.
27515282	0	30	theme	Toll-like	60:68	arg1	signaling					81:89	Toll-like receptor 4 signaling	60:89	Toll-like receptor 4 signaling in cardiomyocytes	60:107	The cytoprotective effect of biglycan core protein involves Toll-like receptor 4 signaling in cardiomyocytes.
27515282	1	31	theme	action	346:351	arg1	clear					360:364	clear	360:364	clear	360:364	AIMS Exogenously administered biglycan (core protein with high-molecular weight glycosaminoglycan chains) has been shown to protect neonatal cardiomyocytes against simulated ischemia/reperfusion injury (SI/R), however, the mechanism of action is not clear.
27515282	1	31	theme	action	346:351	arg1	mechanism					333:341	the mechanism	329:341	the mechanism of action	329:351	AIMS Exogenously administered biglycan (core protein with high-molecular weight glycosaminoglycan chains) has been shown to protect neonatal cardiomyocytes against simulated ischemia/reperfusion injury (SI/R), however, the mechanism of action is not clear.
27515282	1	32	theme	high-molecular	168:181	arg1	chains					208:213	high-molecular weight glycosaminoglycan chains	168:213	high-molecular weight glycosaminoglycan chains	168:213	AIMS Exogenously administered biglycan (core protein with high-molecular weight glycosaminoglycan chains) has been shown to protect neonatal cardiomyocytes against simulated ischemia/reperfusion injury (SI/R), however, the mechanism of action is not clear.
27515282	6	33	theme	SI/R	1238:1241	arg1	effect					1228:1233	the effect	1224:1233	the effect of SI/R upon markers of necrosis, apoptosis, mitochondrial membrane potential, and autophagy	1224:1326	Treatment of cardiomyocytes with rhBGNc reverses the effect of SI/R upon markers of necrosis, apoptosis, mitochondrial membrane potential, and autophagy.
27515282	1	34	dep	biglycan	140:147	arg1	protein					155:161	core protein	150:161	core protein with high-molecular weight glycosaminoglycan chains	150:213	AIMS Exogenously administered biglycan (core protein with high-molecular weight glycosaminoglycan chains) has been shown to protect neonatal cardiomyocytes against simulated ischemia/reperfusion injury (SI/R), however, the mechanism of action is not clear.
27515282	1	35	with	protein	155:161	arg1	chains					208:213	high-molecular weight glycosaminoglycan chains	168:213	high-molecular weight glycosaminoglycan chains	168:213	AIMS Exogenously administered biglycan (core protein with high-molecular weight glycosaminoglycan chains) has been shown to protect neonatal cardiomyocytes against simulated ischemia/reperfusion injury (SI/R), however, the mechanism of action is not clear.
27515282	11	36	dep	biglycan	2053:2060	arg1	protects					2062:2069	protects	2062:2069	protects myocardial cells from SI/R injury via TLR4-mediated mechanisms involving activation of ERK, JNK and p38 MAP kinases and increased NO production	2062:2213	CONCLUSIONS The core protein of exogenous biglycan protects myocardial cells from SI/R injury via TLR4-mediated mechanisms involving activation of ERK, JNK and p38 MAP kinases and increased NO production.
27515282	2	37	theme	molecular	523:531	arg1	mechanisms					533:542	the molecular mechanisms	519:542	the molecular mechanisms involved in the cytoprotection	519:573	In this study we aimed to investigate, which structural component of biglycan is responsible for its cardiocytoprotective effect and to further explore the molecular mechanisms involved in the cytoprotection.
27515282	12	38	theme	changes	2289:2295	arg1	modulation					2262:2271	modulation	2262:2271	modulation of SI/R-induced changes in necrosis, apoptosis and autophagy	2262:2332	The cytoprotective effect of rhBGNc is due to modulation of SI/R-induced changes in necrosis, apoptosis and autophagy.
27515282	11	39	theme	JNK	2163:2165	arg1	production					2204:2213	increased NO production	2191:2213	increased NO production	2191:2213	CONCLUSIONS The core protein of exogenous biglycan protects myocardial cells from SI/R injury via TLR4-mediated mechanisms involving activation of ERK, JNK and p38 MAP kinases and increased NO production.
27515282	11	39	theme	JNK	2163:2165	arg1	activation					2144:2153	activation	2144:2153	activation of ERK, JNK and p38 MAP kinases	2144:2185	CONCLUSIONS The core protein of exogenous biglycan protects myocardial cells from SI/R injury via TLR4-mediated mechanisms involving activation of ERK, JNK and p38 MAP kinases and increased NO production.
27515282	2	40	theme	biglycan	436:443	arg1	component					423:431	structural component	412:431	structural component of biglycan	412:443	In this study we aimed to investigate, which structural component of biglycan is responsible for its cardiocytoprotective effect and to further explore the molecular mechanisms involved in the cytoprotection.
27515282	3	41	attach	isolated	798:805	arg2	cardiomyocytes					783:796	primary neonatal cardiomyocytes	766:796	primary neonatal cardiomyocytes isolated from Wistar rats	766:822	METHODS AND RESULTS A pilot study was conducted to demonstrate that both native (glycanated) and deglycanated biglycan can attenuate cell death induced by SI/R in a dose-dependent manner in primary neonatal cardiomyocytes isolated from Wistar rats.
27515282	3	41	attach	isolated	798:805	arg1	rats					819:822	Wistar rats	812:822	Wistar rats	812:822	METHODS AND RESULTS A pilot study was conducted to demonstrate that both native (glycanated) and deglycanated biglycan can attenuate cell death induced by SI/R in a dose-dependent manner in primary neonatal cardiomyocytes isolated from Wistar rats.
27515282	7	42	theme	SI/R	1541:1544	arg1	injury					1546:1551	SI/R injury	1541:1551	SI/R injury	1541:1551	We have also shown that pharmacological blockade of Toll-like receptor 4 (TLR4) signaling or its downstream mediators (IRAK1/4, ERK, JNK and p38 MAP kinases) abolished the cytoprotective effect of rhBGNc against SI/R injury.
27515282	1	43	theme	core	150:153	arg1	protein					155:161	core protein	150:161	core protein with high-molecular weight glycosaminoglycan chains	150:213	AIMS Exogenously administered biglycan (core protein with high-molecular weight glycosaminoglycan chains) has been shown to protect neonatal cardiomyocytes against simulated ischemia/reperfusion injury (SI/R), however, the mechanism of action is not clear.
27515282	12	44	theme	cytoprotective	2220:2233	arg1	due					2255:2257	due	2255:2257	due	2255:2257	The cytoprotective effect of rhBGNc is due to modulation of SI/R-induced changes in necrosis, apoptosis and autophagy.
27515282	12	44	theme	cytoprotective	2220:2233	arg1	effect					2235:2240	The cytoprotective effect	2216:2240	The cytoprotective effect of rhBGNc	2216:2250	The cytoprotective effect of rhBGNc is due to modulation of SI/R-induced changes in necrosis, apoptosis and autophagy.
27515282	0	45	theme	biglycan	29:36	arg1	protein					43:49	biglycan core protein	29:49	biglycan core protein	29:49	The cytoprotective effect of biglycan core protein involves Toll-like receptor 4 signaling in cardiomyocytes.
27515282	7	46	theme	rhBGNc	1526:1531	arg1	effect					1516:1521	the cytoprotective effect	1497:1521	the cytoprotective effect of rhBGNc against SI/R injury	1497:1551	We have also shown that pharmacological blockade of Toll-like receptor 4 (TLR4) signaling or its downstream mediators (IRAK1/4, ERK, JNK and p38 MAP kinases) abolished the cytoprotective effect of rhBGNc against SI/R injury.
27515282	0	47	theme	protein	43:49	arg1	effect					19:24	The cytoprotective effect	0:24	The cytoprotective effect of biglycan core protein	0:49	The cytoprotective effect of biglycan core protein involves Toll-like receptor 4 signaling in cardiomyocytes.
27515282	3	48	dep	native	649:654	arg1	glycanated					657:666	glycanated	657:666	glycanated	657:666	METHODS AND RESULTS A pilot study was conducted to demonstrate that both native (glycanated) and deglycanated biglycan can attenuate cell death induced by SI/R in a dose-dependent manner in primary neonatal cardiomyocytes isolated from Wistar rats.
27515282	9	49	theme	superoxide	1880:1889	arg1	production					1891:1900	superoxide production	1880:1900	superoxide production	1880:1900	Treatment over 10min and 1h with rhBGNc increased ERK1 phosphorylation, while the SI/R-induced increase in superoxide production was attenuated by rhBGNc.
27515282	3	50	theme	deglycanated	673:684	arg1	biglycan					686:693	both native (glycanated) and deglycanated biglycan	644:693	both native (glycanated) and deglycanated biglycan	644:693	METHODS AND RESULTS A pilot study was conducted to demonstrate that both native (glycanated) and deglycanated biglycan can attenuate cell death induced by SI/R in a dose-dependent manner in primary neonatal cardiomyocytes isolated from Wistar rats.
27515282	8	51	theme	significant	1680:1690	arg1	effect					1692:1697	significant effect	1680:1697	significant effect on phosphorylation of ERK1/2, STAT3, and on the production of superoxide	1680:1770	Pretreatment of cardiomyocytes with rhBGNc for 20h resulted in increased Akt phosphorylation and NO production without having significant effect on phosphorylation of ERK1/2, STAT3, and on the production of superoxide.
27515282	5	52	theme	component	1032:1040	arg1	sulfate					1051:1057	the glycosaminoglycan component dermatan sulfate	1010:1057	the glycosaminoglycan component dermatan sulfate	1010:1057	In contrast, the glycosaminoglycan component dermatan sulfate had no significant effect on cell viability, while chondroitin sulfate further enhanced cell death induced by SI/R.
27515282	8	53	theme	increased	1617:1625	arg1	phosphorylation					1631:1645	increased Akt phosphorylation	1617:1645	increased Akt phosphorylation	1617:1645	Pretreatment of cardiomyocytes with rhBGNc for 20h resulted in increased Akt phosphorylation and NO production without having significant effect on phosphorylation of ERK1/2, STAT3, and on the production of superoxide.
27515282	6	54	theme	autophagy	1318:1326	arg1	markers					1248:1254	markers	1248:1254	markers of necrosis, apoptosis, mitochondrial membrane potential, and autophagy	1248:1326	Treatment of cardiomyocytes with rhBGNc reverses the effect of SI/R upon markers of necrosis, apoptosis, mitochondrial membrane potential, and autophagy.
27515282	1	55	theme	simulated	274:282	arg1	SI/R					313:316	SI/R	313:316	SI/R	313:316	AIMS Exogenously administered biglycan (core protein with high-molecular weight glycosaminoglycan chains) has been shown to protect neonatal cardiomyocytes against simulated ischemia/reperfusion injury (SI/R), however, the mechanism of action is not clear.
27515282	1	55	theme	simulated	274:282	arg1	injury					305:310	simulated ischemia/reperfusion injury	274:310	simulated ischemia/reperfusion injury (SI/R)	274:317	AIMS Exogenously administered biglycan (core protein with high-molecular weight glycosaminoglycan chains) has been shown to protect neonatal cardiomyocytes against simulated ischemia/reperfusion injury (SI/R), however, the mechanism of action is not clear.
27515282	7	56	theme	MAP	1474:1476	arg1	kinases					1478:1484	MAP kinases	1474:1484	MAP kinases	1474:1484	We have also shown that pharmacological blockade of Toll-like receptor 4 (TLR4) signaling or its downstream mediators (IRAK1/4, ERK, JNK and p38 MAP kinases) abolished the cytoprotective effect of rhBGNc against SI/R injury.
27515282	3	57	theme	cell	709:712	arg1	death					714:718	cell death	709:718	cell death induced by SI/R in a dose-dependent manner in primary neonatal cardiomyocytes isolated from Wistar rats	709:822	METHODS AND RESULTS A pilot study was conducted to demonstrate that both native (glycanated) and deglycanated biglycan can attenuate cell death induced by SI/R in a dose-dependent manner in primary neonatal cardiomyocytes isolated from Wistar rats.
27515282	11	58	theme	exogenous	2043:2051	arg1	biglycan					2053:2060	exogenous biglycan	2043:2060	exogenous biglycan protects myocardial cells from SI/R injury via TLR4-mediated mechanisms involving activation of ERK, JNK and p38 MAP kinases and increased NO production	2043:2213	CONCLUSIONS The core protein of exogenous biglycan protects myocardial cells from SI/R injury via TLR4-mediated mechanisms involving activation of ERK, JNK and p38 MAP kinases and increased NO production.
27515282	6	59	theme	cardiomyocytes	1188:1201	arg1	Treatment					1175:1183	Treatment	1175:1183	Treatment of cardiomyocytes with rhBGNc	1175:1213	Treatment of cardiomyocytes with rhBGNc reverses the effect of SI/R upon markers of necrosis, apoptosis, mitochondrial membrane potential, and autophagy.
27515282	8	60	theme	NO	1651:1652	arg1	production					1654:1663	NO production	1651:1663	NO production	1651:1663	Pretreatment of cardiomyocytes with rhBGNc for 20h resulted in increased Akt phosphorylation and NO production without having significant effect on phosphorylation of ERK1/2, STAT3, and on the production of superoxide.
27515282	6	61	theme	membrane	1294:1301	arg1	potential					1303:1311	mitochondrial membrane potential	1280:1311	mitochondrial membrane potential	1280:1311	Treatment of cardiomyocytes with rhBGNc reverses the effect of SI/R upon markers of necrosis, apoptosis, mitochondrial membrane potential, and autophagy.
27515282	1	62	theme	weight	183:188	arg1	chains					208:213	high-molecular weight glycosaminoglycan chains	168:213	high-molecular weight glycosaminoglycan chains	168:213	AIMS Exogenously administered biglycan (core protein with high-molecular weight glycosaminoglycan chains) has been shown to protect neonatal cardiomyocytes against simulated ischemia/reperfusion injury (SI/R), however, the mechanism of action is not clear.
27515282	11	63	dep	ERK	2158:2160	arg1	kinases					2179:2185	MAP kinases	2175:2185	MAP kinases	2175:2185	CONCLUSIONS The core protein of exogenous biglycan protects myocardial cells from SI/R injury via TLR4-mediated mechanisms involving activation of ERK, JNK and p38 MAP kinases and increased NO production.
27515282	10	64	theme	synthesis	1943:1951	arg1	Blockade					1928:1935	Blockade	1928:1935	Blockade of NO synthesis	1928:1951	Blockade of NO synthesis also prevented the cardiocytoprotective effect of rhBGNc.
27515282	12	65	from	modulation	2262:2271	arg1	autophagy					2324:2332	autophagy	2324:2332	autophagy	2324:2332	The cytoprotective effect of rhBGNc is due to modulation of SI/R-induced changes in necrosis, apoptosis and autophagy.
27515282	12	65	from	modulation	2262:2271	arg1	apoptosis					2310:2318	apoptosis	2310:2318	apoptosis	2310:2318	The cytoprotective effect of rhBGNc is due to modulation of SI/R-induced changes in necrosis, apoptosis and autophagy.
27515282	12	65	from	modulation	2262:2271	arg1	necrosis					2300:2307	necrosis	2300:2307	necrosis	2300:2307	The cytoprotective effect of rhBGNc is due to modulation of SI/R-induced changes in necrosis, apoptosis and autophagy.
27515282	11	66	theme	MAP	2175:2177	arg1	kinases					2179:2185	MAP kinases	2175:2185	MAP kinases	2175:2185	CONCLUSIONS The core protein of exogenous biglycan protects myocardial cells from SI/R injury via TLR4-mediated mechanisms involving activation of ERK, JNK and p38 MAP kinases and increased NO production.
27515282	7	67	theme	mediators	1437:1445	arg1	blockade					1369:1376	pharmacological blockade	1353:1376	pharmacological blockade of Toll-like receptor 4 (TLR4) signaling or its downstream mediators (IRAK1/4, ERK, JNK and p38 MAP kinases)	1353:1485	We have also shown that pharmacological blockade of Toll-like receptor 4 (TLR4) signaling or its downstream mediators (IRAK1/4, ERK, JNK and p38 MAP kinases) abolished the cytoprotective effect of rhBGNc against SI/R injury.
27515282	3	68	theme	primary	766:772	arg1	cardiomyocytes					783:796	primary neonatal cardiomyocytes	766:796	primary neonatal cardiomyocytes isolated from Wistar rats	766:822	METHODS AND RESULTS A pilot study was conducted to demonstrate that both native (glycanated) and deglycanated biglycan can attenuate cell death induced by SI/R in a dose-dependent manner in primary neonatal cardiomyocytes isolated from Wistar rats.
27515282	11	69	theme	TLR4-mediated	2109:2121	arg1	mechanisms					2123:2132	TLR4-mediated mechanisms	2109:2132	TLR4-mediated mechanisms involving activation of ERK, JNK and p38 MAP kinases and increased NO production	2109:2213	CONCLUSIONS The core protein of exogenous biglycan protects myocardial cells from SI/R injury via TLR4-mediated mechanisms involving activation of ERK, JNK and p38 MAP kinases and increased NO production.
27515282	0	70	from	signaling	81:89	arg1	cardiomyocytes					94:107	cardiomyocytes	94:107	cardiomyocytes	94:107	The cytoprotective effect of biglycan core protein involves Toll-like receptor 4 signaling in cardiomyocytes.
27515282	7	71	dep	mediators	1437:1445	arg1	IRAK1/4					1448:1454	IRAK1/4	1448:1454	IRAK1/4	1448:1454	We have also shown that pharmacological blockade of Toll-like receptor 4 (TLR4) signaling or its downstream mediators (IRAK1/4, ERK, JNK and p38 MAP kinases) abolished the cytoprotective effect of rhBGNc against SI/R injury.
27515282	10	72	theme	cardiocytoprotective	1972:1991	arg1	effect					1993:1998	the cardiocytoprotective effect	1968:1998	the cardiocytoprotective effect of rhBGNc	1968:2008	Blockade of NO synthesis also prevented the cardiocytoprotective effect of rhBGNc.
27515282	6	73	with	Treatment	1175:1183	arg1	rhBGNc					1208:1213	rhBGNc	1208:1213	rhBGNc	1208:1213	Treatment of cardiomyocytes with rhBGNc reverses the effect of SI/R upon markers of necrosis, apoptosis, mitochondrial membrane potential, and autophagy.
27515282	6	74	theme	necrosis	1259:1266	arg1	markers					1248:1254	markers	1248:1254	markers of necrosis, apoptosis, mitochondrial membrane potential, and autophagy	1248:1326	Treatment of cardiomyocytes with rhBGNc reverses the effect of SI/R upon markers of necrosis, apoptosis, mitochondrial membrane potential, and autophagy.
27515282	5	75	theme	chondroitin	1110:1120	arg1	sulfate					1122:1128	chondroitin sulfate	1110:1128	chondroitin sulfate	1110:1128	In contrast, the glycosaminoglycan component dermatan sulfate had no significant effect on cell viability, while chondroitin sulfate further enhanced cell death induced by SI/R.
27515282	8	76	with	Pretreatment	1554:1565	arg1	rhBGNc					1590:1595	rhBGNc	1590:1595	rhBGNc for 20h	1590:1603	Pretreatment of cardiomyocytes with rhBGNc for 20h resulted in increased Akt phosphorylation and NO production without having significant effect on phosphorylation of ERK1/2, STAT3, and on the production of superoxide.
27515282	8	77	theme	superoxide	1761:1770	arg1	production					1747:1756	the production	1743:1756	the production of superoxide	1743:1770	Pretreatment of cardiomyocytes with rhBGNc for 20h resulted in increased Akt phosphorylation and NO production without having significant effect on phosphorylation of ERK1/2, STAT3, and on the production of superoxide.
27515282	4	78	theme	recombinant	903:913	arg1	rhBGNc					944:949	rhBGNc	944:949	rhBGNc	944:949	In separate experiments, we have shown that similarly to glycanated biglycan, recombinant human biglycan core protein (rhBGNc) protects cardiomyocytes against SI/R injury.
27515282	4	78	theme	recombinant	903:913	arg1	protein					935:941	recombinant human biglycan core protein	903:941	recombinant human biglycan core protein (rhBGNc)	903:950	In separate experiments, we have shown that similarly to glycanated biglycan, recombinant human biglycan core protein (rhBGNc) protects cardiomyocytes against SI/R injury.
27515282	9	79	with	Treatment	1773:1781	arg1	rhBGNc					1806:1811	rhBGNc	1806:1811	rhBGNc	1806:1811	Treatment over 10min and 1h with rhBGNc increased ERK1 phosphorylation, while the SI/R-induced increase in superoxide production was attenuated by rhBGNc.
27515282	4	80	theme	biglycan	921:928	arg1	rhBGNc					944:949	rhBGNc	944:949	rhBGNc	944:949	In separate experiments, we have shown that similarly to glycanated biglycan, recombinant human biglycan core protein (rhBGNc) protects cardiomyocytes against SI/R injury.
27515282	4	80	theme	biglycan	921:928	arg1	protein					935:941	recombinant human biglycan core protein	903:941	recombinant human biglycan core protein (rhBGNc)	903:950	In separate experiments, we have shown that similarly to glycanated biglycan, recombinant human biglycan core protein (rhBGNc) protects cardiomyocytes against SI/R injury.
27515282	8	81	from	effect	1692:1697	arg1	phosphorylation					1702:1716	phosphorylation	1702:1716	phosphorylation of ERK1/2, STAT3	1702:1733	Pretreatment of cardiomyocytes with rhBGNc for 20h resulted in increased Akt phosphorylation and NO production without having significant effect on phosphorylation of ERK1/2, STAT3, and on the production of superoxide.
27515282	8	81	from	effect	1692:1697	arg1	production					1747:1756	the production	1743:1756	the production of superoxide	1743:1770	Pretreatment of cardiomyocytes with rhBGNc for 20h resulted in increased Akt phosphorylation and NO production without having significant effect on phosphorylation of ERK1/2, STAT3, and on the production of superoxide.
27515282	0	82	theme	receptor	70:77	arg1	signaling					81:89	Toll-like receptor 4 signaling	60:89	Toll-like receptor 4 signaling in cardiomyocytes	60:107	The cytoprotective effect of biglycan core protein involves Toll-like receptor 4 signaling in cardiomyocytes.
27515282	5	83	theme	cell	1088:1091	arg1	viability					1093:1101	cell viability	1088:1101	cell viability	1088:1101	In contrast, the glycosaminoglycan component dermatan sulfate had no significant effect on cell viability, while chondroitin sulfate further enhanced cell death induced by SI/R.
27515282	3	84	dep	METHODS	576:582	arg1	conducted					614:622	conducted	614:622	was conducted to demonstrate that both native (glycanated) and deglycanated biglycan can attenuate cell death induced by SI/R in a dose-dependent manner in primary neonatal cardiomyocytes isolated from Wistar rats	610:822	METHODS AND RESULTS A pilot study was conducted to demonstrate that both native (glycanated) and deglycanated biglycan can attenuate cell death induced by SI/R in a dose-dependent manner in primary neonatal cardiomyocytes isolated from Wistar rats.
27515282	7	85	theme	Toll-like	1381:1389	arg1	receptor					1391:1398	Toll-like receptor 4	1381:1400	Toll-like receptor 4 (TLR4) signaling	1381:1417	We have also shown that pharmacological blockade of Toll-like receptor 4 (TLR4) signaling or its downstream mediators (IRAK1/4, ERK, JNK and p38 MAP kinases) abolished the cytoprotective effect of rhBGNc against SI/R injury.
27515282	7	85	theme	Toll-like	1381:1389	arg1	TLR4					1403:1406	TLR4	1403:1406	TLR4	1403:1406	We have also shown that pharmacological blockade of Toll-like receptor 4 (TLR4) signaling or its downstream mediators (IRAK1/4, ERK, JNK and p38 MAP kinases) abolished the cytoprotective effect of rhBGNc against SI/R injury.
27515282	1	86	dep	AIMS	110:113	arg1	biglycan					140:147	biglycan	140:147	AIMS Exogenously administered biglycan (core protein with high-molecular weight glycosaminoglycan chains)	110:214	AIMS Exogenously administered biglycan (core protein with high-molecular weight glycosaminoglycan chains) has been shown to protect neonatal cardiomyocytes against simulated ischemia/reperfusion injury (SI/R), however, the mechanism of action is not clear.
27515282	12	87	from	autophagy	2324:2332	arg1	modulation					2262:2271	modulation	2262:2271	modulation of SI/R-induced changes in necrosis, apoptosis and autophagy	2262:2332	The cytoprotective effect of rhBGNc is due to modulation of SI/R-induced changes in necrosis, apoptosis and autophagy.
27515282	12	88	from	apoptosis	2310:2318	arg1	modulation					2262:2271	modulation	2262:2271	modulation of SI/R-induced changes in necrosis, apoptosis and autophagy	2262:2332	The cytoprotective effect of rhBGNc is due to modulation of SI/R-induced changes in necrosis, apoptosis and autophagy.
27515282	12	89	from	necrosis	2300:2307	arg1	modulation					2262:2271	modulation	2262:2271	modulation of SI/R-induced changes in necrosis, apoptosis and autophagy	2262:2332	The cytoprotective effect of rhBGNc is due to modulation of SI/R-induced changes in necrosis, apoptosis and autophagy.
27515282	7	90	theme	pharmacological	1353:1367	arg1	blockade					1369:1376	pharmacological blockade	1353:1376	pharmacological blockade of Toll-like receptor 4 (TLR4) signaling or its downstream mediators (IRAK1/4, ERK, JNK and p38 MAP kinases)	1353:1485	We have also shown that pharmacological blockade of Toll-like receptor 4 (TLR4) signaling or its downstream mediators (IRAK1/4, ERK, JNK and p38 MAP kinases) abolished the cytoprotective effect of rhBGNc against SI/R injury.
27515282	3	91	from	manner	756:761	arg1	cardiomyocytes					783:796	primary neonatal cardiomyocytes	766:796	primary neonatal cardiomyocytes isolated from Wistar rats	766:822	METHODS AND RESULTS A pilot study was conducted to demonstrate that both native (glycanated) and deglycanated biglycan can attenuate cell death induced by SI/R in a dose-dependent manner in primary neonatal cardiomyocytes isolated from Wistar rats.
27515282	12	92	theme	SI/R-induced	2276:2287	arg1	changes					2289:2295	SI/R-induced changes	2276:2295	SI/R-induced changes in necrosis, apoptosis and autophagy	2276:2332	The cytoprotective effect of rhBGNc is due to modulation of SI/R-induced changes in necrosis, apoptosis and autophagy.
27515282	4	93	theme	SI/R	984:987	arg1	injury					989:994	SI/R injury	984:994	SI/R injury	984:994	In separate experiments, we have shown that similarly to glycanated biglycan, recombinant human biglycan core protein (rhBGNc) protects cardiomyocytes against SI/R injury.
27515282	0	94	theme	cytoprotective	4:17	arg1	effect					19:24	The cytoprotective effect	0:24	The cytoprotective effect of biglycan core protein	0:49	The cytoprotective effect of biglycan core protein involves Toll-like receptor 4 signaling in cardiomyocytes.
27515282	5	95	theme	cell	1147:1150	arg1	death					1152:1156	cell death	1147:1156	cell death induced by SI/R	1147:1172	In contrast, the glycosaminoglycan component dermatan sulfate had no significant effect on cell viability, while chondroitin sulfate further enhanced cell death induced by SI/R.
27515282	12	96	theme	rhBGNc	2245:2250	arg1	due					2255:2257	due	2255:2257	due	2255:2257	The cytoprotective effect of rhBGNc is due to modulation of SI/R-induced changes in necrosis, apoptosis and autophagy.
27515282	12	96	theme	rhBGNc	2245:2250	arg1	effect					2235:2240	The cytoprotective effect	2216:2240	The cytoprotective effect of rhBGNc	2216:2250	The cytoprotective effect of rhBGNc is due to modulation of SI/R-induced changes in necrosis, apoptosis and autophagy.
27515282	11	97	theme	NO	2201:2202	arg1	production					2204:2213	increased NO production	2191:2213	increased NO production	2191:2213	CONCLUSIONS The core protein of exogenous biglycan protects myocardial cells from SI/R injury via TLR4-mediated mechanisms involving activation of ERK, JNK and p38 MAP kinases and increased NO production.
27515282	8	98	theme	cardiomyocytes	1570:1583	arg1	Pretreatment					1554:1565	Pretreatment	1554:1565	Pretreatment of cardiomyocytes with rhBGNc for 20h	1554:1603	Pretreatment of cardiomyocytes with rhBGNc for 20h resulted in increased Akt phosphorylation and NO production without having significant effect on phosphorylation of ERK1/2, STAT3, and on the production of superoxide.
27515282	2	99	theme	structural	412:421	arg1	component					423:431	structural component	412:431	structural component of biglycan	412:443	In this study we aimed to investigate, which structural component of biglycan is responsible for its cardiocytoprotective effect and to further explore the molecular mechanisms involved in the cytoprotection.
27515282	0	100	theme	core	38:41	arg1	protein					43:49	biglycan core protein	29:49	biglycan core protein	29:49	The cytoprotective effect of biglycan core protein involves Toll-like receptor 4 signaling in cardiomyocytes.
27515282	5	101	theme	dermatan	1042:1049	arg1	sulfate					1051:1057	the glycosaminoglycan component dermatan sulfate	1010:1057	the glycosaminoglycan component dermatan sulfate	1010:1057	In contrast, the glycosaminoglycan component dermatan sulfate had no significant effect on cell viability, while chondroitin sulfate further enhanced cell death induced by SI/R.
27515282	2	102	theme	cardiocytoprotective	468:487	arg1	effect					489:494	its cardiocytoprotective effect	464:494	its cardiocytoprotective effect	464:494	In this study we aimed to investigate, which structural component of biglycan is responsible for its cardiocytoprotective effect and to further explore the molecular mechanisms involved in the cytoprotection.
27515282	3	103	theme	native	649:654	arg1	biglycan					686:693	both native (glycanated) and deglycanated biglycan	644:693	both native (glycanated) and deglycanated biglycan	644:693	METHODS AND RESULTS A pilot study was conducted to demonstrate that both native (glycanated) and deglycanated biglycan can attenuate cell death induced by SI/R in a dose-dependent manner in primary neonatal cardiomyocytes isolated from Wistar rats.
27515282	4	104	theme	separate	828:835	arg1	experiments					837:847	separate experiments	828:847	separate experiments	828:847	In separate experiments, we have shown that similarly to glycanated biglycan, recombinant human biglycan core protein (rhBGNc) protects cardiomyocytes against SI/R injury.
27515282	9	105	theme	SI/R-induced	1855:1866	arg1	increase					1868:1875	the SI/R-induced increase	1851:1875	the SI/R-induced increase in superoxide production	1851:1900	Treatment over 10min and 1h with rhBGNc increased ERK1 phosphorylation, while the SI/R-induced increase in superoxide production was attenuated by rhBGNc.
27515282	7	106	theme	signaling	1409:1417	arg1	blockade					1369:1376	pharmacological blockade	1353:1376	pharmacological blockade of Toll-like receptor 4 (TLR4) signaling or its downstream mediators (IRAK1/4, ERK, JNK and p38 MAP kinases)	1353:1485	We have also shown that pharmacological blockade of Toll-like receptor 4 (TLR4) signaling or its downstream mediators (IRAK1/4, ERK, JNK and p38 MAP kinases) abolished the cytoprotective effect of rhBGNc against SI/R injury.
27515282	5	107	contain	had	1059:1061	arg1	sulfate					1051:1057	the glycosaminoglycan component dermatan sulfate	1010:1057	the glycosaminoglycan component dermatan sulfate	1010:1057	In contrast, the glycosaminoglycan component dermatan sulfate had no significant effect on cell viability, while chondroitin sulfate further enhanced cell death induced by SI/R.
27515282	5	107	contain	had	1059:1061	arg2	effect					1078:1083	no significant effect	1063:1083	no significant effect	1063:1083	In contrast, the glycosaminoglycan component dermatan sulfate had no significant effect on cell viability, while chondroitin sulfate further enhanced cell death induced by SI/R.
27515282	1	108	theme	neonatal	242:249	arg1	cardiomyocytes					251:264	neonatal cardiomyocytes	242:264	neonatal cardiomyocytes	242:264	AIMS Exogenously administered biglycan (core protein with high-molecular weight glycosaminoglycan chains) has been shown to protect neonatal cardiomyocytes against simulated ischemia/reperfusion injury (SI/R), however, the mechanism of action is not clear.
27515282	7	109	theme	cytoprotective	1501:1514	arg1	effect					1516:1521	the cytoprotective effect	1497:1521	the cytoprotective effect of rhBGNc against SI/R injury	1497:1551	We have also shown that pharmacological blockade of Toll-like receptor 4 (TLR4) signaling or its downstream mediators (IRAK1/4, ERK, JNK and p38 MAP kinases) abolished the cytoprotective effect of rhBGNc against SI/R injury.
27515282	5	110	theme	glycosaminoglycan	1014:1030	arg1	sulfate					1051:1057	the glycosaminoglycan component dermatan sulfate	1010:1057	the glycosaminoglycan component dermatan sulfate	1010:1057	In contrast, the glycosaminoglycan component dermatan sulfate had no significant effect on cell viability, while chondroitin sulfate further enhanced cell death induced by SI/R.
27515282	8	111	theme	Akt	1627:1629	arg1	phosphorylation					1631:1645	increased Akt phosphorylation	1617:1645	increased Akt phosphorylation	1617:1645	Pretreatment of cardiomyocytes with rhBGNc for 20h resulted in increased Akt phosphorylation and NO production without having significant effect on phosphorylation of ERK1/2, STAT3, and on the production of superoxide.
27515282	10	112	theme	NO	1940:1941	arg1	synthesis					1943:1951	NO synthesis	1940:1951	NO synthesis	1940:1951	Blockade of NO synthesis also prevented the cardiocytoprotective effect of rhBGNc.
26325080	0	0	theme	macromolecular	82:95	arg1	agents					106:111	macromolecular crowding agents	82:111	macromolecular crowding agents alone	82:117	Relationship between protein stability and functional activity in the presence of macromolecular crowding agents alone and in mixture: An insight into stability-activity trade-off.
26325080	7	1	theme	crowding	1219:1226	arg1	dextran					1236:1242	dextran 70	1236:1245	dextran 70	1236:1245	Mixed macromolecular crowding exerts greater effect than the sum of constituent crowding agents (dextran 70 and ficoll 70).
26325080	7	1	theme	crowding	1219:1226	arg1	ficoll					1251:1256	ficoll 70	1251:1259	ficoll 70	1251:1259	Mixed macromolecular crowding exerts greater effect than the sum of constituent crowding agents (dextran 70 and ficoll 70).
26325080	7	1	theme	crowding	1219:1226	arg1	agents					1228:1233	constituent crowding agents	1207:1233	constituent crowding agents (dextran 70 and ficoll 70)	1207:1260	Mixed macromolecular crowding exerts greater effect than the sum of constituent crowding agents (dextran 70 and ficoll 70).
26325080	1	2	theme	varying	256:262	arg1	sizes					264:268	varying sizes	256:268	varying sizes	256:268	The cellular environment is crowded with different kinds of molecules with varying sizes, shapes and compositions.
26325080	2	3	theme	in vivo	467:473	arg1	situation					475:483	the in vivo situation	463:483	the in vivo situation	463:483	Most of the experiments studying the nature and behaviour of a protein have been done on the isolated protein in dilute buffer solutions which actually do not imitate the in vivo situation.
26325080	5	4	theme	mixtures	862:869	arg1	effect					852:857	the effect	848:857	the effect of mixtures of both the crowding agents	848:897	To mimic the cellular condition from physiological point of view, the effect of mixtures of both the crowding agents has been also studied.
26325080	5	5	theme	agents	892:897	arg1	mixtures					862:869	mixtures	862:869	mixtures of both the crowding agents	862:897	To mimic the cellular condition from physiological point of view, the effect of mixtures of both the crowding agents has been also studied.
26325080	0	6	theme	agents	106:111	arg1	presence					70:77	the presence	66:77	the presence of macromolecular crowding agents alone	66:117	Relationship between protein stability and functional activity in the presence of macromolecular crowding agents alone and in mixture: An insight into stability-activity trade-off.
26325080	7	7	dep	agents	1228:1233	arg1	dextran					1236:1242	dextran 70	1236:1245	dextran 70	1236:1245	Mixed macromolecular crowding exerts greater effect than the sum of constituent crowding agents (dextran 70 and ficoll 70).
26325080	7	7	dep	agents	1228:1233	arg1	ficoll					1251:1256	ficoll 70	1251:1259	ficoll 70	1251:1259	Mixed macromolecular crowding exerts greater effect than the sum of constituent crowding agents (dextran 70 and ficoll 70).
26325080	7	7	dep	agents	1228:1233	arg1	agents					1228:1233	constituent crowding agents	1207:1233	constituent crowding agents (dextran 70 and ficoll 70)	1207:1260	Mixed macromolecular crowding exerts greater effect than the sum of constituent crowding agents (dextran 70 and ficoll 70).
26325080	3	8	theme	environment	533:543	arg1	consequences					504:515	the consequences	500:515	the consequences of such crowded environment	500:543	To understand the consequences of such crowded environment, we investigated the effect of macromolecular crowding on the stability and activity of hen egg white lysozyme.
26325080	1	9	with	molecules	241:249	arg1	sizes					264:268	varying sizes	256:268	varying sizes	256:268	The cellular environment is crowded with different kinds of molecules with varying sizes, shapes and compositions.
26325080	1	9	with	molecules	241:249	arg1	compositions					282:293	compositions	282:293	compositions	282:293	The cellular environment is crowded with different kinds of molecules with varying sizes, shapes and compositions.
26325080	1	9	with	molecules	241:249	arg1	shapes					271:276	shapes	271:276	shapes	271:276	The cellular environment is crowded with different kinds of molecules with varying sizes, shapes and compositions.
26325080	7	10	theme	agents	1228:1233	arg1	sum					1200:1202	the sum	1196:1202	the sum of constituent crowding agents (dextran 70 and ficoll 70)	1196:1260	Mixed macromolecular crowding exerts greater effect than the sum of constituent crowding agents (dextran 70 and ficoll 70).
26325080	6	11	theme	volume	957:962	arg1	exclusion					964:972	volume exclusion	957:972	volume exclusion	957:972	The results indicate that owing to volume exclusion, lysozyme is stabilized while its activity decays with the increasing concentration of both the crowders elucidating the hypothesis of stability-activity trade-off.
26325080	0	12	from	activity	54:61	arg1	mixture					126:132	mixture	126:132	mixture	126:132	Relationship between protein stability and functional activity in the presence of macromolecular crowding agents alone and in mixture: An insight into stability-activity trade-off.
26325080	0	12	from	activity	54:61	arg1	presence					70:77	the presence	66:77	the presence of macromolecular crowding agents alone	66:117	Relationship between protein stability and functional activity in the presence of macromolecular crowding agents alone and in mixture: An insight into stability-activity trade-off.
26325080	7	13	theme	Mixed	1139:1143	arg1	crowding					1160:1167	Mixed macromolecular crowding	1139:1167	Mixed macromolecular crowding	1139:1167	Mixed macromolecular crowding exerts greater effect than the sum of constituent crowding agents (dextran 70 and ficoll 70).
26325080	4	14	theme	different	714:722	arg1	shapes					724:729	different shapes	714:729	different shapes	714:729	Two crowding agents, dextran 70 and ficoll 70 which have different shapes and composition, have been employed in this study.
26325080	3	15	theme	such	520:523	arg1	environment					533:543	such crowded environment	520:543	such crowded environment	520:543	To understand the consequences of such crowded environment, we investigated the effect of macromolecular crowding on the stability and activity of hen egg white lysozyme.
26325080	4	16	theme	crowding	661:668	arg1	dextran					678:684	dextran 70	678:687	dextran 70	678:687	Two crowding agents, dextran 70 and ficoll 70 which have different shapes and composition, have been employed in this study.
26325080	4	16	theme	crowding	661:668	arg1	ficoll					693:698	ficoll 70	693:701	ficoll 70	693:701	Two crowding agents, dextran 70 and ficoll 70 which have different shapes and composition, have been employed in this study.
26325080	4	16	theme	crowding	661:668	arg1	agents					670:675	Two crowding agents	657:675	Two crowding agents	657:675	Two crowding agents, dextran 70 and ficoll 70 which have different shapes and composition, have been employed in this study.
26325080	7	17	theme	greater	1176:1182	arg1	effect					1184:1189	greater effect	1176:1189	greater effect	1176:1189	Mixed macromolecular crowding exerts greater effect than the sum of constituent crowding agents (dextran 70 and ficoll 70).
26325080	3	18	theme	crowded	525:531	arg1	environment					533:543	such crowded environment	520:543	such crowded environment	520:543	To understand the consequences of such crowded environment, we investigated the effect of macromolecular crowding on the stability and activity of hen egg white lysozyme.
26325080	7	19	theme	macromolecular	1145:1158	arg1	crowding					1160:1167	Mixed macromolecular crowding	1139:1167	Mixed macromolecular crowding	1139:1167	Mixed macromolecular crowding exerts greater effect than the sum of constituent crowding agents (dextran 70 and ficoll 70).
26325080	6	20	theme	trade-off	1128:1136	arg1	hypothesis					1095:1104	the hypothesis	1091:1104	the hypothesis of stability-activity trade-off	1091:1136	The results indicate that owing to volume exclusion, lysozyme is stabilized while its activity decays with the increasing concentration of both the crowders elucidating the hypothesis of stability-activity trade-off.
26325080	2	21	dep	nature	333:338	arg1	the					329:331	the	329:331	the	329:331	Most of the experiments studying the nature and behaviour of a protein have been done on the isolated protein in dilute buffer solutions which actually do not imitate the in vivo situation.
26325080	1	22	theme	cellular	185:192	arg1	environment					194:204	The cellular environment	181:204	The cellular environment	181:204	The cellular environment is crowded with different kinds of molecules with varying sizes, shapes and compositions.
26325080	6	23	theme	stability-activity	1109:1126	arg1	trade-off					1128:1136	stability-activity trade-off	1109:1136	stability-activity trade-off	1109:1136	The results indicate that owing to volume exclusion, lysozyme is stabilized while its activity decays with the increasing concentration of both the crowders elucidating the hypothesis of stability-activity trade-off.
26325080	2	24	theme	dilute	409:414	arg1	solutions					423:431	dilute buffer solutions	409:431	dilute buffer solutions which actually do not imitate the in vivo situation	409:483	Most of the experiments studying the nature and behaviour of a protein have been done on the isolated protein in dilute buffer solutions which actually do not imitate the in vivo situation.
26325080	2	25	theme	buffer	416:421	arg1	solutions					423:431	dilute buffer solutions	409:431	dilute buffer solutions which actually do not imitate the in vivo situation	409:483	Most of the experiments studying the nature and behaviour of a protein have been done on the isolated protein in dilute buffer solutions which actually do not imitate the in vivo situation.
26325080	4	26	contain	have	709:712	arg1	dextran					678:684	dextran 70	678:687	dextran 70	678:687	Two crowding agents, dextran 70 and ficoll 70 which have different shapes and composition, have been employed in this study.
26325080	4	26	contain	have	709:712	arg1	ficoll					693:698	ficoll 70	693:701	ficoll 70	693:701	Two crowding agents, dextran 70 and ficoll 70 which have different shapes and composition, have been employed in this study.
26325080	4	26	contain	have	709:712	arg1	agents					670:675	Two crowding agents	657:675	Two crowding agents	657:675	Two crowding agents, dextran 70 and ficoll 70 which have different shapes and composition, have been employed in this study.
26325080	4	26	contain	have	709:712	arg2	composition					735:745	composition	735:745	composition	735:745	Two crowding agents, dextran 70 and ficoll 70 which have different shapes and composition, have been employed in this study.
26325080	4	26	contain	have	709:712	arg2	shapes					724:729	different shapes	714:729	different shapes	714:729	Two crowding agents, dextran 70 and ficoll 70 which have different shapes and composition, have been employed in this study.
26325080	3	27	theme	egg	637:639	arg1	lysozyme					647:654	hen egg white lysozyme	633:654	hen egg white lysozyme	633:654	To understand the consequences of such crowded environment, we investigated the effect of macromolecular crowding on the stability and activity of hen egg white lysozyme.
26325080	6	28	theme	increasing	1033:1042	arg1	concentration					1044:1056	the increasing concentration	1029:1056	the increasing concentration of both the crowders elucidating the hypothesis of stability-activity trade-off	1029:1136	The results indicate that owing to volume exclusion, lysozyme is stabilized while its activity decays with the increasing concentration of both the crowders elucidating the hypothesis of stability-activity trade-off.
26325080	3	29	dep	stability	607:615	arg1	the					603:605	the	603:605	the	603:605	To understand the consequences of such crowded environment, we investigated the effect of macromolecular crowding on the stability and activity of hen egg white lysozyme.
26325080	0	30	theme	functional	43:52	arg1	activity					54:61	functional activity	43:61	functional activity	43:61	Relationship between protein stability and functional activity in the presence of macromolecular crowding agents alone and in mixture: An insight into stability-activity trade-off.
26325080	3	31	theme	white	641:645	arg1	lysozyme					647:654	hen egg white lysozyme	633:654	hen egg white lysozyme	633:654	To understand the consequences of such crowded environment, we investigated the effect of macromolecular crowding on the stability and activity of hen egg white lysozyme.
26325080	3	32	theme	crowding	591:598	arg1	effect					566:571	the effect	562:571	the effect of macromolecular crowding on the stability and activity of hen egg white lysozyme	562:654	To understand the consequences of such crowded environment, we investigated the effect of macromolecular crowding on the stability and activity of hen egg white lysozyme.
26325080	0	33	theme	protein	21:27	arg1	stability					29:37	protein stability	21:37	protein stability	21:37	Relationship between protein stability and functional activity in the presence of macromolecular crowding agents alone and in mixture: An insight into stability-activity trade-off.
26325080	5	34	theme	view	842:845	arg1	point					833:837	physiological point	819:837	physiological point of view	819:845	To mimic the cellular condition from physiological point of view, the effect of mixtures of both the crowding agents has been also studied.
26325080	5	35	theme	physiological	819:831	arg1	point					833:837	physiological point	819:837	physiological point of view	819:845	To mimic the cellular condition from physiological point of view, the effect of mixtures of both the crowding agents has been also studied.
26325080	2	36	theme	isolated	389:396	arg1	protein					398:404	the isolated protein	385:404	the isolated protein in dilute buffer solutions which actually do not imitate the in vivo situation	385:483	Most of the experiments studying the nature and behaviour of a protein have been done on the isolated protein in dilute buffer solutions which actually do not imitate the in vivo situation.
26325080	3	37	theme	hen	633:635	arg1	lysozyme					647:654	hen egg white lysozyme	633:654	hen egg white lysozyme	633:654	To understand the consequences of such crowded environment, we investigated the effect of macromolecular crowding on the stability and activity of hen egg white lysozyme.
26325080	3	38	theme	macromolecular	576:589	arg1	crowding					591:598	macromolecular crowding	576:598	macromolecular crowding	576:598	To understand the consequences of such crowded environment, we investigated the effect of macromolecular crowding on the stability and activity of hen egg white lysozyme.
26325080	6	39	theme	crowders	1070:1077	arg1	concentration					1044:1056	the increasing concentration	1029:1056	the increasing concentration of both the crowders elucidating the hypothesis of stability-activity trade-off	1029:1136	The results indicate that owing to volume exclusion, lysozyme is stabilized while its activity decays with the increasing concentration of both the crowders elucidating the hypothesis of stability-activity trade-off.
26325080	0	40	theme	stability-activity	151:168	arg1	trade-off					170:178	stability-activity trade-off	151:178	stability-activity trade-off	151:178	Relationship between protein stability and functional activity in the presence of macromolecular crowding agents alone and in mixture: An insight into stability-activity trade-off.
26325080	3	41	theme	lysozyme	647:654	arg1	stability					607:615	stability	607:615	stability	607:615	To understand the consequences of such crowded environment, we investigated the effect of macromolecular crowding on the stability and activity of hen egg white lysozyme.
26325080	3	41	theme	lysozyme	647:654	arg1	activity					621:628	activity	621:628	activity	621:628	To understand the consequences of such crowded environment, we investigated the effect of macromolecular crowding on the stability and activity of hen egg white lysozyme.
26325080	2	42	from	protein	398:404	arg1	solutions					423:431	dilute buffer solutions	409:431	dilute buffer solutions which actually do not imitate the in vivo situation	409:483	Most of the experiments studying the nature and behaviour of a protein have been done on the isolated protein in dilute buffer solutions which actually do not imitate the in vivo situation.
26325080	5	43	theme	crowding	883:890	arg1	agents					892:897	both the crowding agents	874:897	both the crowding agents	874:897	To mimic the cellular condition from physiological point of view, the effect of mixtures of both the crowding agents has been also studied.
26325080	1	44	theme	different	222:230	arg1	kinds					232:236	different kinds	222:236	different kinds of molecules with varying sizes, shapes and compositions	222:293	The cellular environment is crowded with different kinds of molecules with varying sizes, shapes and compositions.
26325080	5	45	theme	cellular	795:802	arg1	condition					804:812	the cellular condition	791:812	the cellular condition	791:812	To mimic the cellular condition from physiological point of view, the effect of mixtures of both the crowding agents has been also studied.
26325080	3	46	from	effect	566:571	arg1	stability					607:615	stability	607:615	stability	607:615	To understand the consequences of such crowded environment, we investigated the effect of macromolecular crowding on the stability and activity of hen egg white lysozyme.
26325080	3	46	from	effect	566:571	arg1	activity					621:628	activity	621:628	activity	621:628	To understand the consequences of such crowded environment, we investigated the effect of macromolecular crowding on the stability and activity of hen egg white lysozyme.
26325080	1	47	theme	molecules	241:249	arg1	kinds					232:236	different kinds	222:236	different kinds of molecules with varying sizes, shapes and compositions	222:293	The cellular environment is crowded with different kinds of molecules with varying sizes, shapes and compositions.
26325080	7	48	theme	constituent	1207:1217	arg1	dextran					1236:1242	dextran 70	1236:1245	dextran 70	1236:1245	Mixed macromolecular crowding exerts greater effect than the sum of constituent crowding agents (dextran 70 and ficoll 70).
26325080	7	48	theme	constituent	1207:1217	arg1	ficoll					1251:1256	ficoll 70	1251:1259	ficoll 70	1251:1259	Mixed macromolecular crowding exerts greater effect than the sum of constituent crowding agents (dextran 70 and ficoll 70).
26325080	7	48	theme	constituent	1207:1217	arg1	agents					1228:1233	constituent crowding agents	1207:1233	constituent crowding agents (dextran 70 and ficoll 70)	1207:1260	Mixed macromolecular crowding exerts greater effect than the sum of constituent crowding agents (dextran 70 and ficoll 70).
26325080	0	49	from	stability	29:37	arg1	mixture					126:132	mixture	126:132	mixture	126:132	Relationship between protein stability and functional activity in the presence of macromolecular crowding agents alone and in mixture: An insight into stability-activity trade-off.
26325080	0	49	from	stability	29:37	arg1	presence					70:77	the presence	66:77	the presence of macromolecular crowding agents alone	66:117	Relationship between protein stability and functional activity in the presence of macromolecular crowding agents alone and in mixture: An insight into stability-activity trade-off.
26325080	0	50	dep	Relationship	0:11	arg1	insight					138:144	An insight	135:144	Relationship between protein stability and functional activity in the presence of macromolecular crowding agents alone and in mixture: An insight into stability-activity trade-off.	0:179	Relationship between protein stability and functional activity in the presence of macromolecular crowding agents alone and in mixture: An insight into stability-activity trade-off.
26325080	2	51	theme	protein	359:365	arg1	behaviour					344:352	behaviour	344:352	behaviour	344:352	Most of the experiments studying the nature and behaviour of a protein have been done on the isolated protein in dilute buffer solutions which actually do not imitate the in vivo situation.
26325080	2	51	theme	protein	359:365	arg1	nature					333:338	nature	333:338	nature	333:338	Most of the experiments studying the nature and behaviour of a protein have been done on the isolated protein in dilute buffer solutions which actually do not imitate the in vivo situation.
26325080	0	52	theme	crowding	97:104	arg1	agents					106:111	macromolecular crowding agents	82:111	macromolecular crowding agents alone	82:117	Relationship between protein stability and functional activity in the presence of macromolecular crowding agents alone and in mixture: An insight into stability-activity trade-off.
27310468	6	0	theme	reduced	907:913	arg1	levels					922:927	reduced oxygen levels	907:927	reduced oxygen levels	907:927	Targeted transcriptomics and metabolomics confirmed that reduced oxygen levels resulted in increased mannose levels, a shift towards glycolysis, and increased oxidative stress response similar to the manufacturing scale.
27310468	1	1	gly	sialylated	135:144	arg1	protein					156:162	a sialylated Fc-fusion protein	133:162	a sialylated Fc-fusion protein	133:162	A Chinese hamster ovary (CHO) bioprocess, where the product is a sialylated Fc-fusion protein, was operated at pilot and manufacturing scale and significant variation of sialylation level was observed.
27310468	1	1	gly	sialylated	135:144	arg1	product					122:128	the product	118:128	the product	118:128	A Chinese hamster ovary (CHO) bioprocess, where the product is a sialylated Fc-fusion protein, was operated at pilot and manufacturing scale and significant variation of sialylation level was observed.
27310468	3	2	from	runs	481:484	arg1	samples					452:458	samples	452:458	samples from the large scale runs	452:484	Untargeted metabolomics and transcriptomics methods were applied to select samples from the large scale runs.
27310468	9	3	theme	structures	1619:1628	arg1	N-acetylglucosamine					1533:1551	N-acetylglucosamine	1533:1551	N-acetylglucosamine	1533:1551	The lower flux through the hexosamine pathway and reduced intracellular pools of acetyl-CoA led to reduced formation of N-acetylglucosamine and N-acetylneuraminic acid, both key building blocks of N-glycan structures.
27310468	9	3	theme	structures	1619:1628	arg1	acid					1576:1579	N-acetylneuraminic acid	1557:1579	N-acetylneuraminic acid	1557:1579	The lower flux through the hexosamine pathway and reduced intracellular pools of acetyl-CoA led to reduced formation of N-acetylglucosamine and N-acetylneuraminic acid, both key building blocks of N-glycan structures.
27310468	9	3	theme	structures	1619:1628	arg1	blocks					1600:1605	key building blocks	1587:1605	key building blocks of N-glycan structures	1587:1628	The lower flux through the hexosamine pathway and reduced intracellular pools of acetyl-CoA led to reduced formation of N-acetylglucosamine and N-acetylneuraminic acid, both key building blocks of N-glycan structures.
27310468	9	4	theme	reduced	1463:1469	arg1	pools					1485:1489	reduced intracellular pools	1463:1489	reduced intracellular pools of acetyl-CoA	1463:1503	The lower flux through the hexosamine pathway and reduced intracellular pools of acetyl-CoA led to reduced formation of N-acetylglucosamine and N-acetylneuraminic acid, both key building blocks of N-glycan structures.
27310468	9	5	theme	hexosamine	1440:1449	arg1	pathway					1451:1457	the hexosamine pathway	1436:1457	the hexosamine pathway	1436:1457	The lower flux through the hexosamine pathway and reduced intracellular pools of acetyl-CoA led to reduced formation of N-acetylglucosamine and N-acetylneuraminic acid, both key building blocks of N-glycan structures.
27310468	1	6	theme	Fc-fusion	146:154	arg1	protein					156:162	a sialylated Fc-fusion protein	133:162	a sialylated Fc-fusion protein	133:162	A Chinese hamster ovary (CHO) bioprocess, where the product is a sialylated Fc-fusion protein, was operated at pilot and manufacturing scale and significant variation of sialylation level was observed.
27310468	1	6	theme	Fc-fusion	146:154	arg1	product					122:128	the product	118:128	the product	118:128	A Chinese hamster ovary (CHO) bioprocess, where the product is a sialylated Fc-fusion protein, was operated at pilot and manufacturing scale and significant variation of sialylation level was observed.
27310468	6	7	theme	Targeted	850:857	arg1	transcriptomics					859:873	transcriptomics	859:873	transcriptomics	859:873	Targeted transcriptomics and metabolomics confirmed that reduced oxygen levels resulted in increased mannose levels, a shift towards glycolysis, and increased oxidative stress response similar to the manufacturing scale.
27310468	11	8	theme	laboratory	1837:1846	arg1	scales					1848:1853	laboratory scales	1837:1853	laboratory scales	1837:1853	In this study, process, analytical, metabolomic, and transcriptomic data at manufacturing, pilot, and laboratory scales were taken together to develop a systems level understanding of the process and identify oxygen limitation as the root cause of glycosylation variability.
27310468	5	9	theme	5-L	630:632	arg1	model					640:644	a 5-L scale model	628:644	a 5-L scale model operated with a reduced dissolved oxygen set point	628:695	Using a 5-L scale model operated with a reduced dissolved oxygen set point, we were able to reproduce the phenotypic profiles observed at manufacturing scale including lower sialylation, higher lactate and lower ammonia levels.
27310468	8	10	theme	transfer	1205:1212	arg1	limitations					1214:1224	gas transfer limitations	1201:1224	gas transfer limitations at large scale and the presence of oxygen dead-zones inducing upregulation of glycolysis and mannose biosynthesis, and downregulation of hexosamine biosynthesis and acetyl-CoA formation	1201:1410	Oxidative stress results from gas transfer limitations at large scale and the presence of oxygen dead-zones inducing upregulation of glycolysis and mannose biosynthesis, and downregulation of hexosamine biosynthesis and acetyl-CoA formation.
27310468	8	11	theme	dead-zones	1268:1277	arg1	presence					1249:1256	the presence	1245:1256	the presence of oxygen dead-zones inducing upregulation of glycolysis and mannose biosynthesis, and downregulation of hexosamine biosynthesis and acetyl-CoA formation	1245:1410	Oxidative stress results from gas transfer limitations at large scale and the presence of oxygen dead-zones inducing upregulation of glycolysis and mannose biosynthesis, and downregulation of hexosamine biosynthesis and acetyl-CoA formation.
27310468	8	11	theme	dead-zones	1268:1277	arg1	scale					1235:1239	large scale	1229:1239	large scale	1229:1239	Oxidative stress results from gas transfer limitations at large scale and the presence of oxygen dead-zones inducing upregulation of glycolysis and mannose biosynthesis, and downregulation of hexosamine biosynthesis and acetyl-CoA formation.
27310468	5	12	theme	oxygen	680:685	arg1	point					691:695	a reduced dissolved oxygen set point	660:695	a reduced dissolved oxygen set point	660:695	Using a 5-L scale model operated with a reduced dissolved oxygen set point, we were able to reproduce the phenotypic profiles observed at manufacturing scale including lower sialylation, higher lactate and lower ammonia levels.
27310468	4	13	from	response	612:619	arg1	metabolism					569:578	glucose metabolism	561:578	glucose metabolism	561:578	Lower sialylation was correlated with elevated mannose levels, a shift in glucose metabolism, and increased oxidative stress response.
27310468	5	14	theme	higher	809:814	arg1	lactate					816:822	higher lactate	809:822	higher lactate	809:822	Using a 5-L scale model operated with a reduced dissolved oxygen set point, we were able to reproduce the phenotypic profiles observed at manufacturing scale including lower sialylation, higher lactate and lower ammonia levels.
27310468	6	15	theme	mannose	951:957	arg1	levels					959:964	increased mannose levels	941:964	increased mannose levels	941:964	Targeted transcriptomics and metabolomics confirmed that reduced oxygen levels resulted in increased mannose levels, a shift towards glycolysis, and increased oxidative stress response similar to the manufacturing scale.
27310468	5	16	theme	lower	790:794	arg1	sialylation					796:806	lower sialylation	790:806	lower sialylation	790:806	Using a 5-L scale model operated with a reduced dissolved oxygen set point, we were able to reproduce the phenotypic profiles observed at manufacturing scale including lower sialylation, higher lactate and lower ammonia levels.
27310468	1	17	theme	pilot	181:185	arg1	scale					205:209	pilot and manufacturing scale	181:209	scale	205:209	A Chinese hamster ovary (CHO) bioprocess, where the product is a sialylated Fc-fusion protein, was operated at pilot and manufacturing scale and significant variation of sialylation level was observed.
27310468	7	18	theme	large	1122:1126	arg1	operation					1134:1142	large scale operation	1122:1142	large scale operation	1122:1142	Finally, we propose a biological mechanism linking large scale operation and sialylation variation.
27310468	8	19	theme	glycolysis	1304:1313	arg1	downregulation					1345:1358	downregulation	1345:1358	downregulation of hexosamine biosynthesis and acetyl-CoA formation	1345:1410	Oxidative stress results from gas transfer limitations at large scale and the presence of oxygen dead-zones inducing upregulation of glycolysis and mannose biosynthesis, and downregulation of hexosamine biosynthesis and acetyl-CoA formation.
27310468	8	19	theme	glycolysis	1304:1313	arg1	upregulation					1288:1299	upregulation	1288:1299	upregulation of glycolysis and mannose biosynthesis	1288:1338	Oxidative stress results from gas transfer limitations at large scale and the presence of oxygen dead-zones inducing upregulation of glycolysis and mannose biosynthesis, and downregulation of hexosamine biosynthesis and acetyl-CoA formation.
27310468	11	20	theme	glycosylation	1983:1995	arg1	variability					1997:2007	glycosylation variability	1983:2007	glycosylation variability	1983:2007	In this study, process, analytical, metabolomic, and transcriptomic data at manufacturing, pilot, and laboratory scales were taken together to develop a systems level understanding of the process and identify oxygen limitation as the root cause of glycosylation variability.
27310468	7	21	theme	biological	1093:1102	arg1	mechanism					1104:1112	a biological mechanism	1091:1112	a biological mechanism linking large scale operation and sialylation variation	1091:1168	Finally, we propose a biological mechanism linking large scale operation and sialylation variation.
27310468	8	22	theme	mannose	1319:1325	arg1	biosynthesis					1327:1338	mannose biosynthesis	1319:1338	mannose biosynthesis	1319:1338	Oxidative stress results from gas transfer limitations at large scale and the presence of oxygen dead-zones inducing upregulation of glycolysis and mannose biosynthesis, and downregulation of hexosamine biosynthesis and acetyl-CoA formation.
27310468	11	23	theme	transcriptomic	1788:1801	arg1	data					1803:1806	process, analytical, metabolomic, and transcriptomic data	1750:1806	process, analytical, metabolomic, and transcriptomic data at manufacturing, pilot, and laboratory scales	1750:1853	In this study, process, analytical, metabolomic, and transcriptomic data at manufacturing, pilot, and laboratory scales were taken together to develop a systems level understanding of the process and identify oxygen limitation as the root cause of glycosylation variability.
27310468	5	24	theme	reduced	662:668	arg1	point					691:695	a reduced dissolved oxygen set point	660:695	a reduced dissolved oxygen set point	660:695	Using a 5-L scale model operated with a reduced dissolved oxygen set point, we were able to reproduce the phenotypic profiles observed at manufacturing scale including lower sialylation, higher lactate and lower ammonia levels.
27310468	9	25	theme	N-acetylneuraminic	1557:1574	arg1	acid					1576:1579	N-acetylneuraminic acid	1557:1579	N-acetylneuraminic acid	1557:1579	The lower flux through the hexosamine pathway and reduced intracellular pools of acetyl-CoA led to reduced formation of N-acetylglucosamine and N-acetylneuraminic acid, both key building blocks of N-glycan structures.
27310468	6	26	theme	oxidative	1009:1017	arg1	response					1026:1033	increased oxidative stress response	999:1033	increased oxidative stress response	999:1033	Targeted transcriptomics and metabolomics confirmed that reduced oxygen levels resulted in increased mannose levels, a shift towards glycolysis, and increased oxidative stress response similar to the manufacturing scale.
27310468	9	27	theme	N-acetylglucosamine	1533:1551	arg1	formation					1520:1528	reduced formation	1512:1528	reduced formation of N-acetylglucosamine and N-acetylneuraminic acid, both key building blocks of N-glycan structures	1512:1628	The lower flux through the hexosamine pathway and reduced intracellular pools of acetyl-CoA led to reduced formation of N-acetylglucosamine and N-acetylneuraminic acid, both key building blocks of N-glycan structures.
27310468	5	28	theme	phenotypic	728:737	arg1	profiles					739:746	the phenotypic profiles	724:746	the phenotypic profiles observed at manufacturing scale including lower sialylation, higher lactate and lower ammonia levels	724:847	Using a 5-L scale model operated with a reduced dissolved oxygen set point, we were able to reproduce the phenotypic profiles observed at manufacturing scale including lower sialylation, higher lactate and lower ammonia levels.
27310468	9	29	theme	key	1587:1589	arg1	blocks					1600:1605	key building blocks	1587:1605	key building blocks of N-glycan structures	1587:1628	The lower flux through the hexosamine pathway and reduced intracellular pools of acetyl-CoA led to reduced formation of N-acetylglucosamine and N-acetylneuraminic acid, both key building blocks of N-glycan structures.
27310468	4	30	theme	Lower	487:491	arg1	sialylation					493:503	Lower sialylation	487:503	Lower sialylation	487:503	Lower sialylation was correlated with elevated mannose levels, a shift in glucose metabolism, and increased oxidative stress response.
27310468	8	31	theme	hexosamine	1363:1372	arg1	biosynthesis					1374:1385	hexosamine biosynthesis	1363:1385	hexosamine biosynthesis	1363:1385	Oxidative stress results from gas transfer limitations at large scale and the presence of oxygen dead-zones inducing upregulation of glycolysis and mannose biosynthesis, and downregulation of hexosamine biosynthesis and acetyl-CoA formation.
27310468	9	32	theme	blocks	1600:1605	arg1	formation					1520:1528	reduced formation	1512:1528	reduced formation of N-acetylglucosamine and N-acetylneuraminic acid, both key building blocks of N-glycan structures	1512:1628	The lower flux through the hexosamine pathway and reduced intracellular pools of acetyl-CoA led to reduced formation of N-acetylglucosamine and N-acetylneuraminic acid, both key building blocks of N-glycan structures.
27310468	10	33	theme	mammalian	1705:1713	arg1	sialyation					1723:1732	mammalian protein sialyation	1705:1732	mammalian protein sialyation	1705:1732	This study reports for the first time a link between oxidative stress and mammalian protein sialyation.
27310468	4	34	from	shift	552:556	arg1	metabolism					569:578	glucose metabolism	561:578	glucose metabolism	561:578	Lower sialylation was correlated with elevated mannose levels, a shift in glucose metabolism, and increased oxidative stress response.
27310468	5	35	located	observed	748:755	arg2	profiles					739:746	the phenotypic profiles	724:746	the phenotypic profiles observed at manufacturing scale including lower sialylation, higher lactate and lower ammonia levels	724:847	Using a 5-L scale model operated with a reduced dissolved oxygen set point, we were able to reproduce the phenotypic profiles observed at manufacturing scale including lower sialylation, higher lactate and lower ammonia levels.
27310468	5	35	located	observed	748:755	arg1	manufacturing					760:772	manufacturing scale	760:778	manufacturing scale including lower sialylation, higher lactate and lower ammonia levels	760:847	Using a 5-L scale model operated with a reduced dissolved oxygen set point, we were able to reproduce the phenotypic profiles observed at manufacturing scale including lower sialylation, higher lactate and lower ammonia levels.
27310468	1	36	theme	Chinese	72:78	arg1	ovary					88:92	Chinese hamster ovary	72:92	A Chinese hamster ovary (CHO) bioprocess	70:109	A Chinese hamster ovary (CHO) bioprocess, where the product is a sialylated Fc-fusion protein, was operated at pilot and manufacturing scale and significant variation of sialylation level was observed.
27310468	1	36	theme	Chinese	72:78	arg1	CHO					95:97	CHO	95:97	CHO	95:97	A Chinese hamster ovary (CHO) bioprocess, where the product is a sialylated Fc-fusion protein, was operated at pilot and manufacturing scale and significant variation of sialylation level was observed.
27310468	8	37	theme	formation	1402:1410	arg1	downregulation					1345:1358	downregulation	1345:1358	downregulation of hexosamine biosynthesis and acetyl-CoA formation	1345:1410	Oxidative stress results from gas transfer limitations at large scale and the presence of oxygen dead-zones inducing upregulation of glycolysis and mannose biosynthesis, and downregulation of hexosamine biosynthesis and acetyl-CoA formation.
27310468	8	37	theme	formation	1402:1410	arg1	upregulation					1288:1299	upregulation	1288:1299	upregulation of glycolysis and mannose biosynthesis	1288:1338	Oxidative stress results from gas transfer limitations at large scale and the presence of oxygen dead-zones inducing upregulation of glycolysis and mannose biosynthesis, and downregulation of hexosamine biosynthesis and acetyl-CoA formation.
27310468	1	38	theme	level	252:256	arg1	variation					227:235	significant variation	215:235	significant variation of sialylation level	215:256	A Chinese hamster ovary (CHO) bioprocess, where the product is a sialylated Fc-fusion protein, was operated at pilot and manufacturing scale and significant variation of sialylation level was observed.
27310468	1	39	theme	ovary	88:92	arg1	bioprocess					100:109	A Chinese hamster ovary (CHO) bioprocess	70:109	A Chinese hamster ovary (CHO) bioprocess	70:109	A Chinese hamster ovary (CHO) bioprocess, where the product is a sialylated Fc-fusion protein, was operated at pilot and manufacturing scale and significant variation of sialylation level was observed.
27310468	6	40	theme	manufacturing	1050:1062	arg1	scale					1064:1068	the manufacturing scale	1046:1068	the manufacturing scale	1046:1068	Targeted transcriptomics and metabolomics confirmed that reduced oxygen levels resulted in increased mannose levels, a shift towards glycolysis, and increased oxidative stress response similar to the manufacturing scale.
27310468	4	41	theme	mannose	534:540	arg1	levels					542:547	elevated mannose levels	525:547	elevated mannose levels	525:547	Lower sialylation was correlated with elevated mannose levels, a shift in glucose metabolism, and increased oxidative stress response.
27310468	11	42	theme	root	1969:1972	arg1	limitation					1951:1960	oxygen limitation	1944:1960	oxygen limitation	1944:1960	In this study, process, analytical, metabolomic, and transcriptomic data at manufacturing, pilot, and laboratory scales were taken together to develop a systems level understanding of the process and identify oxygen limitation as the root cause of glycosylation variability.
27310468	11	42	theme	root	1969:1972	arg1	cause					1974:1978	the root cause	1965:1978	the root cause of glycosylation variability	1965:2007	In this study, process, analytical, metabolomic, and transcriptomic data at manufacturing, pilot, and laboratory scales were taken together to develop a systems level understanding of the process and identify oxygen limitation as the root cause of glycosylation variability.
27310468	3	43	theme	Untargeted	377:386	arg1	metabolomics					388:399	metabolomics	388:399	metabolomics	388:399	Untargeted metabolomics and transcriptomics methods were applied to select samples from the large scale runs.
27310468	11	44	theme	process	1750:1756	arg1	data					1803:1806	process, analytical, metabolomic, and transcriptomic data	1750:1806	process, analytical, metabolomic, and transcriptomic data at manufacturing, pilot, and laboratory scales	1750:1853	In this study, process, analytical, metabolomic, and transcriptomic data at manufacturing, pilot, and laboratory scales were taken together to develop a systems level understanding of the process and identify oxygen limitation as the root cause of glycosylation variability.
27310468	9	45	theme	intracellular	1471:1483	arg1	pools					1485:1489	reduced intracellular pools	1463:1489	reduced intracellular pools of acetyl-CoA	1463:1503	The lower flux through the hexosamine pathway and reduced intracellular pools of acetyl-CoA led to reduced formation of N-acetylglucosamine and N-acetylneuraminic acid, both key building blocks of N-glycan structures.
27310468	7	46	theme	sialylation	1148:1158	arg1	variation					1160:1168	sialylation variation	1148:1168	sialylation variation	1148:1168	Finally, we propose a biological mechanism linking large scale operation and sialylation variation.
27310468	3	47	theme	large	469:473	arg1	runs					481:484	the large scale runs	465:484	the large scale runs	465:484	Untargeted metabolomics and transcriptomics methods were applied to select samples from the large scale runs.
27310468	0	48	theme	CHO	47:49	arg1	Process					61:67	a CHO Fc-Fusion Process	45:67	a CHO Fc-Fusion Process	45:67	Understanding and Controlling Sialylation in a CHO Fc-Fusion Process.
27310468	11	49	theme	metabolomic	1771:1781	arg1	process					1750:1756	process	1750:1756	process	1750:1756	In this study, process, analytical, metabolomic, and transcriptomic data at manufacturing, pilot, and laboratory scales were taken together to develop a systems level understanding of the process and identify oxygen limitation as the root cause of glycosylation variability.
27310468	9	50	theme	lower	1417:1421	arg1	flux					1423:1426	The lower flux	1413:1426	The lower flux through the hexosamine pathway and reduced intracellular pools of acetyl-CoA	1413:1503	The lower flux through the hexosamine pathway and reduced intracellular pools of acetyl-CoA led to reduced formation of N-acetylglucosamine and N-acetylneuraminic acid, both key building blocks of N-glycan structures.
27310468	10	51	theme	protein	1715:1721	arg1	sialyation					1723:1732	mammalian protein sialyation	1705:1732	mammalian protein sialyation	1705:1732	This study reports for the first time a link between oxidative stress and mammalian protein sialyation.
27310468	5	52	theme	ammonia	834:840	arg1	levels					842:847	lower ammonia levels	828:847	lower ammonia levels	828:847	Using a 5-L scale model operated with a reduced dissolved oxygen set point, we were able to reproduce the phenotypic profiles observed at manufacturing scale including lower sialylation, higher lactate and lower ammonia levels.
27310468	7	53	theme	scale	1128:1132	arg1	operation					1134:1142	large scale operation	1122:1142	large scale operation	1122:1142	Finally, we propose a biological mechanism linking large scale operation and sialylation variation.
27310468	6	54	theme	oxygen	915:920	arg1	levels					922:927	reduced oxygen levels	907:927	reduced oxygen levels	907:927	Targeted transcriptomics and metabolomics confirmed that reduced oxygen levels resulted in increased mannose levels, a shift towards glycolysis, and increased oxidative stress response similar to the manufacturing scale.
27310468	4	55	theme	increased	585:593	arg1	response					612:619	increased oxidative stress response	585:619	increased oxidative stress response	585:619	Lower sialylation was correlated with elevated mannose levels, a shift in glucose metabolism, and increased oxidative stress response.
27310468	1	56	theme	significant	215:225	arg1	variation					227:235	significant variation	215:235	significant variation of sialylation level	215:256	A Chinese hamster ovary (CHO) bioprocess, where the product is a sialylated Fc-fusion protein, was operated at pilot and manufacturing scale and significant variation of sialylation level was observed.
27310468	5	57	theme	scale	634:638	arg1	model					640:644	a 5-L scale model	628:644	a 5-L scale model operated with a reduced dissolved oxygen set point	628:695	Using a 5-L scale model operated with a reduced dissolved oxygen set point, we were able to reproduce the phenotypic profiles observed at manufacturing scale including lower sialylation, higher lactate and lower ammonia levels.
27310468	8	58	from	scale	1235:1239	arg1	limitations					1214:1224	gas transfer limitations	1201:1224	gas transfer limitations at large scale and the presence of oxygen dead-zones inducing upregulation of glycolysis and mannose biosynthesis, and downregulation of hexosamine biosynthesis and acetyl-CoA formation	1201:1410	Oxidative stress results from gas transfer limitations at large scale and the presence of oxygen dead-zones inducing upregulation of glycolysis and mannose biosynthesis, and downregulation of hexosamine biosynthesis and acetyl-CoA formation.
27310468	4	59	theme	glucose	561:567	arg1	metabolism					569:578	glucose metabolism	561:578	glucose metabolism	561:578	Lower sialylation was correlated with elevated mannose levels, a shift in glucose metabolism, and increased oxidative stress response.
27310468	11	60	theme	systems	1888:1894	arg1	understanding					1902:1914	a systems level understanding	1886:1914	a systems level understanding of the process	1886:1929	In this study, process, analytical, metabolomic, and transcriptomic data at manufacturing, pilot, and laboratory scales were taken together to develop a systems level understanding of the process and identify oxygen limitation as the root cause of glycosylation variability.
27310468	1	61	theme	sialylated	135:144	arg1	protein					156:162	a sialylated Fc-fusion protein	133:162	a sialylated Fc-fusion protein	133:162	A Chinese hamster ovary (CHO) bioprocess, where the product is a sialylated Fc-fusion protein, was operated at pilot and manufacturing scale and significant variation of sialylation level was observed.
27310468	1	61	theme	sialylated	135:144	arg1	product					122:128	the product	118:128	the product	118:128	A Chinese hamster ovary (CHO) bioprocess, where the product is a sialylated Fc-fusion protein, was operated at pilot and manufacturing scale and significant variation of sialylation level was observed.
27310468	8	62	theme	Oxidative	1171:1179	arg1	stress					1181:1186	Oxidative stress	1171:1186	Oxidative stress	1171:1186	Oxidative stress results from gas transfer limitations at large scale and the presence of oxygen dead-zones inducing upregulation of glycolysis and mannose biosynthesis, and downregulation of hexosamine biosynthesis and acetyl-CoA formation.
27310468	10	63	theme	first	1658:1662	arg1	time					1664:1667	the first time	1654:1667	the first time	1654:1667	This study reports for the first time a link between oxidative stress and mammalian protein sialyation.
27310468	11	64	from	scales	1848:1853	arg1	data					1803:1806	process, analytical, metabolomic, and transcriptomic data	1750:1806	process, analytical, metabolomic, and transcriptomic data at manufacturing, pilot, and laboratory scales	1750:1853	In this study, process, analytical, metabolomic, and transcriptomic data at manufacturing, pilot, and laboratory scales were taken together to develop a systems level understanding of the process and identify oxygen limitation as the root cause of glycosylation variability.
27310468	5	65	theme	dissolved	670:678	arg1	oxygen					680:685	dissolved oxygen	670:685	a reduced dissolved oxygen set point	660:695	Using a 5-L scale model operated with a reduced dissolved oxygen set point, we were able to reproduce the phenotypic profiles observed at manufacturing scale including lower sialylation, higher lactate and lower ammonia levels.
27310468	8	66	theme	gas	1201:1203	arg1	limitations					1214:1224	gas transfer limitations	1201:1224	gas transfer limitations at large scale and the presence of oxygen dead-zones inducing upregulation of glycolysis and mannose biosynthesis, and downregulation of hexosamine biosynthesis and acetyl-CoA formation	1201:1410	Oxidative stress results from gas transfer limitations at large scale and the presence of oxygen dead-zones inducing upregulation of glycolysis and mannose biosynthesis, and downregulation of hexosamine biosynthesis and acetyl-CoA formation.
27310468	11	67	from	manufacturing	1811:1823	arg1	data					1803:1806	process, analytical, metabolomic, and transcriptomic data	1750:1806	process, analytical, metabolomic, and transcriptomic data at manufacturing, pilot, and laboratory scales	1750:1853	In this study, process, analytical, metabolomic, and transcriptomic data at manufacturing, pilot, and laboratory scales were taken together to develop a systems level understanding of the process and identify oxygen limitation as the root cause of glycosylation variability.
27310468	8	68	theme	oxygen	1261:1266	arg1	dead-zones					1268:1277	oxygen dead-zones	1261:1277	oxygen dead-zones inducing upregulation of glycolysis and mannose biosynthesis, and downregulation of hexosamine biosynthesis and acetyl-CoA formation	1261:1410	Oxidative stress results from gas transfer limitations at large scale and the presence of oxygen dead-zones inducing upregulation of glycolysis and mannose biosynthesis, and downregulation of hexosamine biosynthesis and acetyl-CoA formation.
27310468	3	69	theme	scale	475:479	arg1	runs					481:484	the large scale runs	465:484	the large scale runs	465:484	Untargeted metabolomics and transcriptomics methods were applied to select samples from the large scale runs.
27310468	5	70	theme	set	687:689	arg1	point					691:695	a reduced dissolved oxygen set point	660:695	a reduced dissolved oxygen set point	660:695	Using a 5-L scale model operated with a reduced dissolved oxygen set point, we were able to reproduce the phenotypic profiles observed at manufacturing scale including lower sialylation, higher lactate and lower ammonia levels.
27310468	11	71	theme	variability	1997:2007	arg1	limitation					1951:1960	oxygen limitation	1944:1960	oxygen limitation	1944:1960	In this study, process, analytical, metabolomic, and transcriptomic data at manufacturing, pilot, and laboratory scales were taken together to develop a systems level understanding of the process and identify oxygen limitation as the root cause of glycosylation variability.
27310468	11	71	theme	variability	1997:2007	arg1	cause					1974:1978	the root cause	1965:1978	the root cause of glycosylation variability	1965:2007	In this study, process, analytical, metabolomic, and transcriptomic data at manufacturing, pilot, and laboratory scales were taken together to develop a systems level understanding of the process and identify oxygen limitation as the root cause of glycosylation variability.
27310468	9	72	theme	acetyl-CoA	1494:1503	arg1	pathway					1451:1457	the hexosamine pathway	1436:1457	the hexosamine pathway	1436:1457	The lower flux through the hexosamine pathway and reduced intracellular pools of acetyl-CoA led to reduced formation of N-acetylglucosamine and N-acetylneuraminic acid, both key building blocks of N-glycan structures.
27310468	9	72	theme	acetyl-CoA	1494:1503	arg1	pools					1485:1489	reduced intracellular pools	1463:1489	reduced intracellular pools of acetyl-CoA	1463:1503	The lower flux through the hexosamine pathway and reduced intracellular pools of acetyl-CoA led to reduced formation of N-acetylglucosamine and N-acetylneuraminic acid, both key building blocks of N-glycan structures.
27310468	9	73	theme	reduced	1512:1518	arg1	formation					1520:1528	reduced formation	1512:1528	reduced formation of N-acetylglucosamine and N-acetylneuraminic acid, both key building blocks of N-glycan structures	1512:1628	The lower flux through the hexosamine pathway and reduced intracellular pools of acetyl-CoA led to reduced formation of N-acetylglucosamine and N-acetylneuraminic acid, both key building blocks of N-glycan structures.
27310468	6	74	theme	increased	941:949	arg1	levels					959:964	increased mannose levels	941:964	increased mannose levels	941:964	Targeted transcriptomics and metabolomics confirmed that reduced oxygen levels resulted in increased mannose levels, a shift towards glycolysis, and increased oxidative stress response similar to the manufacturing scale.
27310468	11	75	from	pilot	1826:1830	arg1	data					1803:1806	process, analytical, metabolomic, and transcriptomic data	1750:1806	process, analytical, metabolomic, and transcriptomic data at manufacturing, pilot, and laboratory scales	1750:1853	In this study, process, analytical, metabolomic, and transcriptomic data at manufacturing, pilot, and laboratory scales were taken together to develop a systems level understanding of the process and identify oxygen limitation as the root cause of glycosylation variability.
27310468	8	76	from	presence	1249:1256	arg1	limitations					1214:1224	gas transfer limitations	1201:1224	gas transfer limitations at large scale and the presence of oxygen dead-zones inducing upregulation of glycolysis and mannose biosynthesis, and downregulation of hexosamine biosynthesis and acetyl-CoA formation	1201:1410	Oxidative stress results from gas transfer limitations at large scale and the presence of oxygen dead-zones inducing upregulation of glycolysis and mannose biosynthesis, and downregulation of hexosamine biosynthesis and acetyl-CoA formation.
27310468	8	77	theme	large	1229:1233	arg1	scale					1235:1239	large scale	1229:1239	large scale	1229:1239	Oxidative stress results from gas transfer limitations at large scale and the presence of oxygen dead-zones inducing upregulation of glycolysis and mannose biosynthesis, and downregulation of hexosamine biosynthesis and acetyl-CoA formation.
27310468	11	78	theme	analytical	1759:1768	arg1	process					1750:1756	process	1750:1756	process	1750:1756	In this study, process, analytical, metabolomic, and transcriptomic data at manufacturing, pilot, and laboratory scales were taken together to develop a systems level understanding of the process and identify oxygen limitation as the root cause of glycosylation variability.
27310468	6	79	theme	stress	1019:1024	arg1	response					1026:1033	increased oxidative stress response	999:1033	increased oxidative stress response	999:1033	Targeted transcriptomics and metabolomics confirmed that reduced oxygen levels resulted in increased mannose levels, a shift towards glycolysis, and increased oxidative stress response similar to the manufacturing scale.
27310468	9	80	dep	N-acetylglucosamine	1533:1551	arg1	both					1582:1585	both	1582:1585	both	1582:1585	The lower flux through the hexosamine pathway and reduced intracellular pools of acetyl-CoA led to reduced formation of N-acetylglucosamine and N-acetylneuraminic acid, both key building blocks of N-glycan structures.
27310468	1	81	theme	sialylation	240:250	arg1	level					252:256	sialylation level	240:256	sialylation level	240:256	A Chinese hamster ovary (CHO) bioprocess, where the product is a sialylated Fc-fusion protein, was operated at pilot and manufacturing scale and significant variation of sialylation level was observed.
27310468	9	82	theme	acid	1576:1579	arg1	formation					1520:1528	reduced formation	1512:1528	reduced formation of N-acetylglucosamine and N-acetylneuraminic acid, both key building blocks of N-glycan structures	1512:1628	The lower flux through the hexosamine pathway and reduced intracellular pools of acetyl-CoA led to reduced formation of N-acetylglucosamine and N-acetylneuraminic acid, both key building blocks of N-glycan structures.
27310468	6	83	theme	increased	999:1007	arg1	response					1026:1033	increased oxidative stress response	999:1033	increased oxidative stress response	999:1033	Targeted transcriptomics and metabolomics confirmed that reduced oxygen levels resulted in increased mannose levels, a shift towards glycolysis, and increased oxidative stress response similar to the manufacturing scale.
27310468	8	84	theme	biosynthesis	1327:1338	arg1	downregulation					1345:1358	downregulation	1345:1358	downregulation of hexosamine biosynthesis and acetyl-CoA formation	1345:1410	Oxidative stress results from gas transfer limitations at large scale and the presence of oxygen dead-zones inducing upregulation of glycolysis and mannose biosynthesis, and downregulation of hexosamine biosynthesis and acetyl-CoA formation.
27310468	8	84	theme	biosynthesis	1327:1338	arg1	upregulation					1288:1299	upregulation	1288:1299	upregulation of glycolysis and mannose biosynthesis	1288:1338	Oxidative stress results from gas transfer limitations at large scale and the presence of oxygen dead-zones inducing upregulation of glycolysis and mannose biosynthesis, and downregulation of hexosamine biosynthesis and acetyl-CoA formation.
27310468	9	85	theme	building	1591:1598	arg1	blocks					1600:1605	key building blocks	1587:1605	key building blocks of N-glycan structures	1587:1628	The lower flux through the hexosamine pathway and reduced intracellular pools of acetyl-CoA led to reduced formation of N-acetylglucosamine and N-acetylneuraminic acid, both key building blocks of N-glycan structures.
27310468	5	86	theme	lower	828:832	arg1	levels					842:847	lower ammonia levels	828:847	lower ammonia levels	828:847	Using a 5-L scale model operated with a reduced dissolved oxygen set point, we were able to reproduce the phenotypic profiles observed at manufacturing scale including lower sialylation, higher lactate and lower ammonia levels.
27310468	4	87	theme	stress	605:610	arg1	response					612:619	increased oxidative stress response	585:619	increased oxidative stress response	585:619	Lower sialylation was correlated with elevated mannose levels, a shift in glucose metabolism, and increased oxidative stress response.
27310468	8	88	theme	biosynthesis	1374:1385	arg1	downregulation					1345:1358	downregulation	1345:1358	downregulation of hexosamine biosynthesis and acetyl-CoA formation	1345:1410	Oxidative stress results from gas transfer limitations at large scale and the presence of oxygen dead-zones inducing upregulation of glycolysis and mannose biosynthesis, and downregulation of hexosamine biosynthesis and acetyl-CoA formation.
27310468	8	88	theme	biosynthesis	1374:1385	arg1	upregulation					1288:1299	upregulation	1288:1299	upregulation of glycolysis and mannose biosynthesis	1288:1338	Oxidative stress results from gas transfer limitations at large scale and the presence of oxygen dead-zones inducing upregulation of glycolysis and mannose biosynthesis, and downregulation of hexosamine biosynthesis and acetyl-CoA formation.
27310468	5	89	theme	scale	774:778	arg1	manufacturing					760:772	manufacturing scale	760:778	manufacturing scale including lower sialylation, higher lactate and lower ammonia levels	760:847	Using a 5-L scale model operated with a reduced dissolved oxygen set point, we were able to reproduce the phenotypic profiles observed at manufacturing scale including lower sialylation, higher lactate and lower ammonia levels.
27310468	1	90	theme	hamster	80:86	arg1	ovary					88:92	Chinese hamster ovary	72:92	A Chinese hamster ovary (CHO) bioprocess	70:109	A Chinese hamster ovary (CHO) bioprocess, where the product is a sialylated Fc-fusion protein, was operated at pilot and manufacturing scale and significant variation of sialylation level was observed.
27310468	1	90	theme	hamster	80:86	arg1	CHO					95:97	CHO	95:97	CHO	95:97	A Chinese hamster ovary (CHO) bioprocess, where the product is a sialylated Fc-fusion protein, was operated at pilot and manufacturing scale and significant variation of sialylation level was observed.
27310468	3	91	dep	metabolomics	388:399	arg1	methods					421:427	methods	421:427	methods	421:427	Untargeted metabolomics and transcriptomics methods were applied to select samples from the large scale runs.
27310468	8	92	theme	acetyl-CoA	1391:1400	arg1	formation					1402:1410	acetyl-CoA formation	1391:1410	acetyl-CoA formation	1391:1410	Oxidative stress results from gas transfer limitations at large scale and the presence of oxygen dead-zones inducing upregulation of glycolysis and mannose biosynthesis, and downregulation of hexosamine biosynthesis and acetyl-CoA formation.
27310468	10	93	theme	oxidative	1684:1692	arg1	stress					1694:1699	oxidative stress	1684:1699	oxidative stress	1684:1699	This study reports for the first time a link between oxidative stress and mammalian protein sialyation.
27310468	4	94	from	levels	542:547	arg1	metabolism					569:578	glucose metabolism	561:578	glucose metabolism	561:578	Lower sialylation was correlated with elevated mannose levels, a shift in glucose metabolism, and increased oxidative stress response.
27310468	6	95	theme	similar	1035:1041	arg1	levels					959:964	increased mannose levels	941:964	increased mannose levels	941:964	Targeted transcriptomics and metabolomics confirmed that reduced oxygen levels resulted in increased mannose levels, a shift towards glycolysis, and increased oxidative stress response similar to the manufacturing scale.
27310468	11	96	theme	oxygen	1944:1949	arg1	limitation					1951:1960	oxygen limitation	1944:1960	oxygen limitation	1944:1960	In this study, process, analytical, metabolomic, and transcriptomic data at manufacturing, pilot, and laboratory scales were taken together to develop a systems level understanding of the process and identify oxygen limitation as the root cause of glycosylation variability.
27310468	11	96	theme	oxygen	1944:1949	arg1	cause					1974:1978	the root cause	1965:1978	the root cause of glycosylation variability	1965:2007	In this study, process, analytical, metabolomic, and transcriptomic data at manufacturing, pilot, and laboratory scales were taken together to develop a systems level understanding of the process and identify oxygen limitation as the root cause of glycosylation variability.
27310468	2	97	theme	variability	364:374	arg1	cause					355:359	the cause	351:359	the cause of variability	351:374	In order to more tightly control glycosylation profiles, we sought to identify the cause of variability.
27310468	0	98	theme	Fc-Fusion	51:59	arg1	Process					61:67	a CHO Fc-Fusion Process	45:67	a CHO Fc-Fusion Process	45:67	Understanding and Controlling Sialylation in a CHO Fc-Fusion Process.
27310468	4	99	theme	elevated	525:532	arg1	levels					542:547	elevated mannose levels	525:547	elevated mannose levels	525:547	Lower sialylation was correlated with elevated mannose levels, a shift in glucose metabolism, and increased oxidative stress response.
27310468	2	100	theme	glycosylation	305:317	arg1	profiles					319:326	glycosylation profiles	305:326	glycosylation profiles	305:326	In order to more tightly control glycosylation profiles, we sought to identify the cause of variability.
27310468	11	101	theme	level	1896:1900	arg1	understanding					1902:1914	a systems level understanding	1886:1914	a systems level understanding of the process	1886:1929	In this study, process, analytical, metabolomic, and transcriptomic data at manufacturing, pilot, and laboratory scales were taken together to develop a systems level understanding of the process and identify oxygen limitation as the root cause of glycosylation variability.
27310468	9	102	theme	N-glycan	1610:1617	arg1	structures					1619:1628	N-glycan structures	1610:1628	N-glycan structures	1610:1628	The lower flux through the hexosamine pathway and reduced intracellular pools of acetyl-CoA led to reduced formation of N-acetylglucosamine and N-acetylneuraminic acid, both key building blocks of N-glycan structures.
27310468	1	103	theme	manufacturing	191:203	arg1	scale					205:209	pilot and manufacturing scale	181:209	scale	205:209	A Chinese hamster ovary (CHO) bioprocess, where the product is a sialylated Fc-fusion protein, was operated at pilot and manufacturing scale and significant variation of sialylation level was observed.
27310468	4	104	theme	oxidative	595:603	arg1	response					612:619	increased oxidative stress response	585:619	increased oxidative stress response	585:619	Lower sialylation was correlated with elevated mannose levels, a shift in glucose metabolism, and increased oxidative stress response.
27310468	11	105	theme	process	1923:1929	arg1	understanding					1902:1914	a systems level understanding	1886:1914	a systems level understanding of the process	1886:1929	In this study, process, analytical, metabolomic, and transcriptomic data at manufacturing, pilot, and laboratory scales were taken together to develop a systems level understanding of the process and identify oxygen limitation as the root cause of glycosylation variability.
26655459	9	0	theme	high	1737:1740	arg1	concentrations					1749:1762	high tablet concentrations	1737:1762	high tablet concentrations	1737:1762	These models show that the mechanical performance significantly degrades with tablet misalignment, and especially at high tablet concentrations.
26655459	5	1	theme	different	1078:1086	arg1	techniques					1088:1097	different techniques	1078:1097	different techniques	1078:1097	This phenomenon, also reported in the past for most of nacre-like materials, composed of micro/nano tablets, obtained from different techniques, has been limiting our ability to produce large volumes of high-performance nacre-like materials.
26655459	6	2	theme	lower	1256:1260	arg1	concentrations					1269:1282	lower tablet concentrations	1256:1282	lower tablet concentrations	1256:1282	Examination of the structure of the films revealed that at lower tablet concentrations the tablets are well-aligned and well dispersed thorough the volume of the film.
26655459	5	3	theme	micro/nano	1044:1053	arg1	tablets					1055:1061	micro/nano tablets	1044:1061	micro/nano tablets	1044:1061	This phenomenon, also reported in the past for most of nacre-like materials, composed of micro/nano tablets, obtained from different techniques, has been limiting our ability to produce large volumes of high-performance nacre-like materials.
26655459	9	4	theme	tablet	1742:1747	arg1	concentrations					1749:1762	high tablet concentrations	1737:1762	high tablet concentrations	1737:1762	These models show that the mechanical performance significantly degrades with tablet misalignment, and especially at high tablet concentrations.
26655459	3	5	theme	microscopic	671:681	arg1	tablets					691:697	microscopic alumina tablets	671:697	microscopic alumina tablets	671:697	Here, we report the use of a simple and versatile technique based on doctor-blading to fabricate staggered composites of microscopic alumina tablets with high alignment in a chitosan matrix.
26655459	5	6	theme	nacre-like	1010:1019	arg1	materials					1021:1029	nacre-like materials	1010:1029	nacre-like materials	1010:1029	This phenomenon, also reported in the past for most of nacre-like materials, composed of micro/nano tablets, obtained from different techniques, has been limiting our ability to produce large volumes of high-performance nacre-like materials.
26655459	8	7	theme	structure	1586:1594	arg1	representative					1564:1577	representative	1564:1577	representative	1564:1577	In order to investigate the impact of these imperfections on material performance we developed large scale finite element models representative of the structure of the composite films.
26655459	3	8	theme	alumina	683:689	arg1	tablets					691:697	microscopic alumina tablets	671:697	microscopic alumina tablets	671:697	Here, we report the use of a simple and versatile technique based on doctor-blading to fabricate staggered composites of microscopic alumina tablets with high alignment in a chitosan matrix.
26655459	10	9	theme	high	1914:1917	arg1	contents					1926:1933	high tablet contents	1914:1933	high tablet contents	1914:1933	The simulations along with the SEM images therefore quantitatively explain the experimental trends, e.g. the degradation of mechanical properties at high tablet contents.
26655459	1	10	theme	stiff	247:251	arg1	reinforcements					264:277	stiff and strong reinforcements	247:277	stiff and strong reinforcements	247:277	The remarkable mechanical performance of biological materials such as bone, nacre, and spider silk stems from their staggered microstructure in which stiff and strong reinforcements are elongated in the direction of loading, separated by softer interfaces, and shifted relative to each other.
26655459	4	11	theme	15vol	912:916	arg1	%					917:917	15vol%	912:917	15vol% after which all properties degrade	912:952	Tensile tests on these nacre-like materials show that the modulus and strength of the composite films are enhanced by the incorporation of ceramic tablets, but only up to 15vol% after which all properties degrade.
26655459	1	12	theme	softer	335:340	arg1	interfaces					342:351	softer interfaces	335:351	softer interfaces	335:351	The remarkable mechanical performance of biological materials such as bone, nacre, and spider silk stems from their staggered microstructure in which stiff and strong reinforcements are elongated in the direction of loading, separated by softer interfaces, and shifted relative to each other.
26655459	6	13	theme	films	1233:1237	arg1	structure					1216:1224	the structure	1212:1224	the structure of the films	1212:1237	Examination of the structure of the films revealed that at lower tablet concentrations the tablets are well-aligned and well dispersed thorough the volume of the film.
26655459	10	14	from	degradation	1874:1884	arg1	contents					1926:1933	high tablet contents	1914:1933	high tablet contents	1914:1933	The simulations along with the SEM images therefore quantitatively explain the experimental trends, e.g. the degradation of mechanical properties at high tablet contents.
26655459	8	15	theme	finite	1542:1547	arg1	models					1557:1562	large scale finite element models	1530:1562	large scale finite element models representative of the structure of the composite films	1530:1617	In order to investigate the impact of these imperfections on material performance we developed large scale finite element models representative of the structure of the composite films.
26655459	5	16	theme	nacre-like	1175:1184	arg1	materials					1186:1194	high-performance nacre-like materials	1158:1194	high-performance nacre-like materials	1158:1194	This phenomenon, also reported in the past for most of nacre-like materials, composed of micro/nano tablets, obtained from different techniques, has been limiting our ability to produce large volumes of high-performance nacre-like materials.
26655459	4	17	theme	composite	827:835	arg1	films					837:841	the composite films	823:841	the composite films	823:841	Tensile tests on these nacre-like materials show that the modulus and strength of the composite films are enhanced by the incorporation of ceramic tablets, but only up to 15vol% after which all properties degrade.
26655459	4	18	from	tests	749:753	arg1	materials					775:783	these nacre-like materials	758:783	these nacre-like materials	758:783	Tensile tests on these nacre-like materials show that the modulus and strength of the composite films are enhanced by the incorporation of ceramic tablets, but only up to 15vol% after which all properties degrade.
26655459	4	19	theme	nacre-like	764:773	arg1	materials					775:783	these nacre-like materials	758:783	these nacre-like materials	758:783	Tensile tests on these nacre-like materials show that the modulus and strength of the composite films are enhanced by the incorporation of ceramic tablets, but only up to 15vol% after which all properties degrade.
26655459	10	20	theme	tablet	1919:1924	arg1	contents					1926:1933	high tablet contents	1914:1933	high tablet contents	1914:1933	The simulations along with the SEM images therefore quantitatively explain the experimental trends, e.g. the degradation of mechanical properties at high tablet contents.
26655459	3	21	theme	high	704:707	arg1	alignment					709:717	high alignment	704:717	high alignment	704:717	Here, we report the use of a simple and versatile technique based on doctor-blading to fabricate staggered composites of microscopic alumina tablets with high alignment in a chitosan matrix.
26655459	10	22	from	contents	1926:1933	arg1	degradation					1874:1884	the degradation	1870:1884	the degradation of mechanical properties at high tablet contents	1870:1933	The simulations along with the SEM images therefore quantitatively explain the experimental trends, e.g. the degradation of mechanical properties at high tablet contents.
26655459	10	22	from	contents	1926:1933	arg1	properties					1900:1909	mechanical properties	1889:1909	mechanical properties at high tablet contents	1889:1933	The simulations along with the SEM images therefore quantitatively explain the experimental trends, e.g. the degradation of mechanical properties at high tablet contents.
26655459	10	23	theme	properties	1900:1909	arg1	degradation					1874:1884	the degradation	1870:1884	the degradation of mechanical properties at high tablet contents	1870:1933	The simulations along with the SEM images therefore quantitatively explain the experimental trends, e.g. the degradation of mechanical properties at high tablet contents.
26655459	0	24	theme	Nacre-like	0:9	arg1	materials					11:19	Nacre-like materials	0:19	Nacre-like materials using a simple doctor blading technique	0:59	Nacre-like materials using a simple doctor blading technique: Fabrication, testing and modeling.
26655459	3	25	with	composites	657:666	arg1	alignment					709:717	high alignment	704:717	high alignment	704:717	Here, we report the use of a simple and versatile technique based on doctor-blading to fabricate staggered composites of microscopic alumina tablets with high alignment in a chitosan matrix.
26655459	8	26	from	impact	1463:1468	arg1	performance					1505:1515	material performance	1496:1515	material performance	1496:1515	In order to investigate the impact of these imperfections on material performance we developed large scale finite element models representative of the structure of the composite films.
26655459	7	27	theme	tablet	1399:1404	arg1	misalignment					1406:1417	tablet misalignment	1399:1417	tablet misalignment	1399:1417	At 15vol% and beyond, we observed tablet misalignment and clustering.
26655459	6	28	theme	structure	1216:1224	arg1	Examination					1197:1207	Examination	1197:1207	Examination of the structure of the films	1197:1237	Examination of the structure of the films revealed that at lower tablet concentrations the tablets are well-aligned and well dispersed thorough the volume of the film.
26655459	7	29	theme	15vol	1368:1372	arg1	%					1373:1373	15vol%	1368:1373	15vol%	1368:1373	At 15vol% and beyond, we observed tablet misalignment and clustering.
26655459	2	30	theme	useful	416:421	arg1	combinations					423:434	useful combinations	416:434	useful combinations of modulus	416:445	This structure results in useful combinations of modulus, strength and toughness and therefore is increasingly mimicked in bio-inspired engineering composites.
26655459	2	30	theme	useful	416:421	arg1	toughness					461:469	toughness	461:469	toughness	461:469	This structure results in useful combinations of modulus, strength and toughness and therefore is increasingly mimicked in bio-inspired engineering composites.
26655459	2	30	theme	useful	416:421	arg1	strength					448:455	strength	448:455	strength	448:455	This structure results in useful combinations of modulus, strength and toughness and therefore is increasingly mimicked in bio-inspired engineering composites.
26655459	1	31	theme	strong	257:262	arg1	reinforcements					264:277	stiff and strong reinforcements	247:277	stiff and strong reinforcements	247:277	The remarkable mechanical performance of biological materials such as bone, nacre, and spider silk stems from their staggered microstructure in which stiff and strong reinforcements are elongated in the direction of loading, separated by softer interfaces, and shifted relative to each other.
26655459	4	32	theme	films	837:841	arg1	strength					811:818	strength	811:818	strength	811:818	Tensile tests on these nacre-like materials show that the modulus and strength of the composite films are enhanced by the incorporation of ceramic tablets, but only up to 15vol% after which all properties degrade.
26655459	4	32	theme	films	837:841	arg1	modulus					799:805	modulus	799:805	modulus	799:805	Tensile tests on these nacre-like materials show that the modulus and strength of the composite films are enhanced by the incorporation of ceramic tablets, but only up to 15vol% after which all properties degrade.
26655459	8	33	theme	material	1496:1503	arg1	performance					1505:1515	material performance	1496:1515	material performance	1496:1515	In order to investigate the impact of these imperfections on material performance we developed large scale finite element models representative of the structure of the composite films.
26655459	0	34	dep	Fabrication	62:72	arg1	materials					11:19	Nacre-like materials	0:19	Nacre-like materials using a simple doctor blading technique	0:59	Nacre-like materials using a simple doctor blading technique: Fabrication, testing and modeling.
26655459	3	35	theme	chitosan	724:731	arg1	matrix					733:738	a chitosan matrix	722:738	a chitosan matrix	722:738	Here, we report the use of a simple and versatile technique based on doctor-blading to fabricate staggered composites of microscopic alumina tablets with high alignment in a chitosan matrix.
26655459	1	36	theme	spider	184:189	arg1	silk					191:194	spider silk	184:194	spider silk	184:194	The remarkable mechanical performance of biological materials such as bone, nacre, and spider silk stems from their staggered microstructure in which stiff and strong reinforcements are elongated in the direction of loading, separated by softer interfaces, and shifted relative to each other.
26655459	0	37	theme	doctor	36:41	arg1	technique					51:59	a simple doctor blading technique	27:59	a simple doctor blading technique	27:59	Nacre-like materials using a simple doctor blading technique: Fabrication, testing and modeling.
26655459	5	38	theme	large	1141:1145	arg1	volumes					1147:1153	large volumes	1141:1153	large volumes of high-performance nacre-like materials	1141:1194	This phenomenon, also reported in the past for most of nacre-like materials, composed of micro/nano tablets, obtained from different techniques, has been limiting our ability to produce large volumes of high-performance nacre-like materials.
26655459	2	39	theme	engineering	526:536	arg1	composites					538:547	bio-inspired engineering composites	513:547	bio-inspired engineering composites	513:547	This structure results in useful combinations of modulus, strength and toughness and therefore is increasingly mimicked in bio-inspired engineering composites.
26655459	9	40	theme	mechanical	1647:1656	arg1	performance					1658:1668	the mechanical performance	1643:1668	the mechanical performance	1643:1668	These models show that the mechanical performance significantly degrades with tablet misalignment, and especially at high tablet concentrations.
26655459	10	41	theme	SEM	1796:1798	arg1	images					1800:1805	the SEM images	1792:1805	the SEM images	1792:1805	The simulations along with the SEM images therefore quantitatively explain the experimental trends, e.g. the degradation of mechanical properties at high tablet contents.
26655459	9	42	theme	tablet	1698:1703	arg1	misalignment					1705:1716	tablet misalignment	1698:1716	tablet misalignment	1698:1716	These models show that the mechanical performance significantly degrades with tablet misalignment, and especially at high tablet concentrations.
26655459	0	43	theme	simple	29:34	arg1	technique					51:59	a simple doctor blading technique	27:59	a simple doctor blading technique	27:59	Nacre-like materials using a simple doctor blading technique: Fabrication, testing and modeling.
26655459	8	44	theme	scale	1536:1540	arg1	models					1557:1562	large scale finite element models	1530:1562	large scale finite element models representative of the structure of the composite films	1530:1617	In order to investigate the impact of these imperfections on material performance we developed large scale finite element models representative of the structure of the composite films.
26655459	2	45	theme	bio-inspired	513:524	arg1	composites					538:547	bio-inspired engineering composites	513:547	bio-inspired engineering composites	513:547	This structure results in useful combinations of modulus, strength and toughness and therefore is increasingly mimicked in bio-inspired engineering composites.
26655459	10	46	theme	experimental	1844:1855	arg1	trends					1857:1862	the experimental trends	1840:1862	the experimental trends	1840:1862	The simulations along with the SEM images therefore quantitatively explain the experimental trends, e.g. the degradation of mechanical properties at high tablet contents.
26655459	4	47	dep	modulus	799:805	arg1	the					795:797	the	795:797	the	795:797	Tensile tests on these nacre-like materials show that the modulus and strength of the composite films are enhanced by the incorporation of ceramic tablets, but only up to 15vol% after which all properties degrade.
26655459	2	48	theme	modulus	439:445	arg1	combinations					423:434	useful combinations	416:434	useful combinations of modulus	416:445	This structure results in useful combinations of modulus, strength and toughness and therefore is increasingly mimicked in bio-inspired engineering composites.
26655459	2	48	theme	modulus	439:445	arg1	toughness					461:469	toughness	461:469	toughness	461:469	This structure results in useful combinations of modulus, strength and toughness and therefore is increasingly mimicked in bio-inspired engineering composites.
26655459	2	48	theme	modulus	439:445	arg1	strength					448:455	strength	448:455	strength	448:455	This structure results in useful combinations of modulus, strength and toughness and therefore is increasingly mimicked in bio-inspired engineering composites.
26655459	8	49	theme	large	1530:1534	arg1	models					1557:1562	large scale finite element models	1530:1562	large scale finite element models representative of the structure of the composite films	1530:1617	In order to investigate the impact of these imperfections on material performance we developed large scale finite element models representative of the structure of the composite films.
26655459	4	50	theme	ceramic	880:886	arg1	tablets					888:894	ceramic tablets	880:894	ceramic tablets	880:894	Tensile tests on these nacre-like materials show that the modulus and strength of the composite films are enhanced by the incorporation of ceramic tablets, but only up to 15vol% after which all properties degrade.
26655459	1	51	theme	remarkable	101:110	arg1	performance					123:133	The remarkable mechanical performance	97:133	The remarkable mechanical performance of biological materials such as bone, nacre, and spider silk	97:194	The remarkable mechanical performance of biological materials such as bone, nacre, and spider silk stems from their staggered microstructure in which stiff and strong reinforcements are elongated in the direction of loading, separated by softer interfaces, and shifted relative to each other.
26655459	0	52	theme	blading	43:49	arg1	technique					51:59	a simple doctor blading technique	27:59	a simple doctor blading technique	27:59	Nacre-like materials using a simple doctor blading technique: Fabrication, testing and modeling.
26655459	8	53	theme	element	1549:1555	arg1	models					1557:1562	large scale finite element models	1530:1562	large scale finite element models representative of the structure of the composite films	1530:1617	In order to investigate the impact of these imperfections on material performance we developed large scale finite element models representative of the structure of the composite films.
26655459	1	54	theme	loading	313:319	arg1	direction					300:308	the direction	296:308	the direction of loading	296:319	The remarkable mechanical performance of biological materials such as bone, nacre, and spider silk stems from their staggered microstructure in which stiff and strong reinforcements are elongated in the direction of loading, separated by softer interfaces, and shifted relative to each other.
26655459	10	55	theme	mechanical	1889:1898	arg1	properties					1900:1909	mechanical properties	1889:1909	mechanical properties at high tablet contents	1889:1933	The simulations along with the SEM images therefore quantitatively explain the experimental trends, e.g. the degradation of mechanical properties at high tablet contents.
26655459	1	56	theme	mechanical	112:121	arg1	performance					123:133	The remarkable mechanical performance	97:133	The remarkable mechanical performance of biological materials such as bone, nacre, and spider silk	97:194	The remarkable mechanical performance of biological materials such as bone, nacre, and spider silk stems from their staggered microstructure in which stiff and strong reinforcements are elongated in the direction of loading, separated by softer interfaces, and shifted relative to each other.
26655459	6	57	from	concentrations	1269:1282	arg1	well-aligned					1300:1311	well-aligned	1300:1311	well-aligned	1300:1311	Examination of the structure of the films revealed that at lower tablet concentrations the tablets are well-aligned and well dispersed thorough the volume of the film.
26655459	6	58	theme	tablet	1262:1267	arg1	concentrations					1269:1282	lower tablet concentrations	1256:1282	lower tablet concentrations	1256:1282	Examination of the structure of the films revealed that at lower tablet concentrations the tablets are well-aligned and well dispersed thorough the volume of the film.
26655459	3	59	theme	versatile	590:598	arg1	technique					600:608	a simple and versatile technique	577:608	a simple and versatile technique based on doctor-blading	577:632	Here, we report the use of a simple and versatile technique based on doctor-blading to fabricate staggered composites of microscopic alumina tablets with high alignment in a chitosan matrix.
26655459	4	60	theme	Tensile	741:747	arg1	tests					749:753	Tensile tests	741:753	Tensile tests on these nacre-like materials	741:783	Tensile tests on these nacre-like materials show that the modulus and strength of the composite films are enhanced by the incorporation of ceramic tablets, but only up to 15vol% after which all properties degrade.
26655459	5	61	theme	materials	1186:1194	arg1	volumes					1147:1153	large volumes	1141:1153	large volumes of high-performance nacre-like materials	1141:1194	This phenomenon, also reported in the past for most of nacre-like materials, composed of micro/nano tablets, obtained from different techniques, has been limiting our ability to produce large volumes of high-performance nacre-like materials.
26655459	4	62	theme	tablets	888:894	arg1	incorporation					863:875	the incorporation	859:875	the incorporation of ceramic tablets	859:894	Tensile tests on these nacre-like materials show that the modulus and strength of the composite films are enhanced by the incorporation of ceramic tablets, but only up to 15vol% after which all properties degrade.
26655459	3	63	theme	technique	600:608	arg1	use					570:572	the use	566:572	the use of a simple and versatile technique based on doctor-blading to fabricate staggered composites of microscopic alumina tablets with high alignment in a chitosan matrix	566:738	Here, we report the use of a simple and versatile technique based on doctor-blading to fabricate staggered composites of microscopic alumina tablets with high alignment in a chitosan matrix.
26655459	4	64	dep	%	917:917	arg1	up					906:907	up	906:907	up	906:907	Tensile tests on these nacre-like materials show that the modulus and strength of the composite films are enhanced by the incorporation of ceramic tablets, but only up to 15vol% after which all properties degrade.
26655459	8	65	theme	representative	1564:1577	arg1	models					1557:1562	large scale finite element models	1530:1562	large scale finite element models representative of the structure of the composite films	1530:1617	In order to investigate the impact of these imperfections on material performance we developed large scale finite element models representative of the structure of the composite films.
26655459	6	66	theme	film	1359:1362	arg1	volume					1345:1350	the volume	1341:1350	the volume of the film	1341:1362	Examination of the structure of the films revealed that at lower tablet concentrations the tablets are well-aligned and well dispersed thorough the volume of the film.
26655459	3	67	theme	staggered	647:655	arg1	tablets					691:697	microscopic alumina tablets	671:697	microscopic alumina tablets	671:697	Here, we report the use of a simple and versatile technique based on doctor-blading to fabricate staggered composites of microscopic alumina tablets with high alignment in a chitosan matrix.
26655459	3	67	theme	staggered	647:655	arg1	composites					657:666	staggered composites	647:666	staggered composites of microscopic alumina tablets with high alignment	647:717	Here, we report the use of a simple and versatile technique based on doctor-blading to fabricate staggered composites of microscopic alumina tablets with high alignment in a chitosan matrix.
26655459	3	68	theme	simple	579:584	arg1	technique					600:608	a simple and versatile technique	577:608	a simple and versatile technique based on doctor-blading	577:632	Here, we report the use of a simple and versatile technique based on doctor-blading to fabricate staggered composites of microscopic alumina tablets with high alignment in a chitosan matrix.
26655459	8	69	theme	imperfections	1479:1491	arg1	impact					1463:1468	the impact	1459:1468	the impact of these imperfections on material performance	1459:1515	In order to investigate the impact of these imperfections on material performance we developed large scale finite element models representative of the structure of the composite films.
26655459	1	70	theme	biological	138:147	arg1	nacre					173:177	nacre	173:177	nacre	173:177	The remarkable mechanical performance of biological materials such as bone, nacre, and spider silk stems from their staggered microstructure in which stiff and strong reinforcements are elongated in the direction of loading, separated by softer interfaces, and shifted relative to each other.
26655459	1	70	theme	biological	138:147	arg1	materials					149:157	biological materials	138:157	biological materials such as bone, nacre, and spider silk	138:194	The remarkable mechanical performance of biological materials such as bone, nacre, and spider silk stems from their staggered microstructure in which stiff and strong reinforcements are elongated in the direction of loading, separated by softer interfaces, and shifted relative to each other.
26655459	1	70	theme	biological	138:147	arg1	bone					167:170	bone	167:170	bone	167:170	The remarkable mechanical performance of biological materials such as bone, nacre, and spider silk stems from their staggered microstructure in which stiff and strong reinforcements are elongated in the direction of loading, separated by softer interfaces, and shifted relative to each other.
26655459	1	70	theme	biological	138:147	arg1	silk					191:194	spider silk	184:194	spider silk	184:194	The remarkable mechanical performance of biological materials such as bone, nacre, and spider silk stems from their staggered microstructure in which stiff and strong reinforcements are elongated in the direction of loading, separated by softer interfaces, and shifted relative to each other.
26655459	8	71	theme	films	1613:1617	arg1	structure					1586:1594	the structure	1582:1594	the structure of the composite films	1582:1617	In order to investigate the impact of these imperfections on material performance we developed large scale finite element models representative of the structure of the composite films.
26655459	1	72	theme	staggered	213:221	arg1	microstructure					223:236	their staggered microstructure	207:236	their staggered microstructure in which stiff and strong reinforcements are elongated in the direction of loading, separated by softer interfaces, and shifted relative to each other	207:387	The remarkable mechanical performance of biological materials such as bone, nacre, and spider silk stems from their staggered microstructure in which stiff and strong reinforcements are elongated in the direction of loading, separated by softer interfaces, and shifted relative to each other.
26655459	5	73	theme	high-performance	1158:1173	arg1	materials					1186:1194	high-performance nacre-like materials	1158:1194	high-performance nacre-like materials	1158:1194	This phenomenon, also reported in the past for most of nacre-like materials, composed of micro/nano tablets, obtained from different techniques, has been limiting our ability to produce large volumes of high-performance nacre-like materials.
26655459	3	74	theme	tablets	691:697	arg1	tablets					691:697	microscopic alumina tablets	671:697	microscopic alumina tablets	671:697	Here, we report the use of a simple and versatile technique based on doctor-blading to fabricate staggered composites of microscopic alumina tablets with high alignment in a chitosan matrix.
26655459	3	74	theme	tablets	691:697	arg1	composites					657:666	staggered composites	647:666	staggered composites of microscopic alumina tablets with high alignment	647:717	Here, we report the use of a simple and versatile technique based on doctor-blading to fabricate staggered composites of microscopic alumina tablets with high alignment in a chitosan matrix.
26655459	1	75	theme	materials	149:157	arg1	performance					123:133	The remarkable mechanical performance	97:133	The remarkable mechanical performance of biological materials such as bone, nacre, and spider silk	97:194	The remarkable mechanical performance of biological materials such as bone, nacre, and spider silk stems from their staggered microstructure in which stiff and strong reinforcements are elongated in the direction of loading, separated by softer interfaces, and shifted relative to each other.
26655459	8	76	theme	composite	1603:1611	arg1	films					1613:1617	the composite films	1599:1617	the composite films	1599:1617	In order to investigate the impact of these imperfections on material performance we developed large scale finite element models representative of the structure of the composite films.
26655459	10	77	dep	degradation	1874:1884	arg1	e.g.					1865:1868	e.g.	1865:1868	e.g.	1865:1868	The simulations along with the SEM images therefore quantitatively explain the experimental trends, e.g. the degradation of mechanical properties at high tablet contents.
26572391	0	0	theme	Pleurotus	98:106	arg1	djamor					108:113	Pleurotus djamor	98:113	Pleurotus djamor	98:113	Purification, characterization and hepatoprotective activities of mycelia zinc polysaccharides by Pleurotus djamor.
26572391	2	1	theme	In	479:480	arg1	assays					488:493	In vitro assays	479:493	In vitro assays	479:493	In vitro assays, the MZPS-3 demonstrated relatively strong antioxidant activities in dose-dependent manners.
26572391	2	2	theme	antioxidant	538:548	arg1	activities					550:559	relatively strong antioxidant activities	520:559	relatively strong antioxidant activities	520:559	In vitro assays, the MZPS-3 demonstrated relatively strong antioxidant activities in dose-dependent manners.
26572391	1	3	from	properties	175:184	arg1	damage					355:360	carbon tetrachloride (CCl4)-induced acute liver damage	307:360	carbon tetrachloride (CCl4)-induced acute liver damage	307:360	This study was designed to investigate the physicochemical properties (molecular weights, bond types and monosaccharide compositions), antioxidant activities, and hepatoprotective effects on carbon tetrachloride (CCl4)-induced acute liver damage of mycelia zinc polysaccharides (MZPSs) and its major fractions (MZPS-1, -2 and -3) separated from Pleurotus djamor.
26572391	3	4	theme	GSH-Px	827:832	arg1	activities					808:817	the activities	804:817	the activities of SOD, GSH-Px, CAT and T-AOC in serum/liver homogenate against CCl4-induced injures	804:902	For in vivo hepatoprotective activities, administration of MZPS-3 at 800 mg/kg significantly decreased the levels of AST, ALT, MDA and LPO, remarkably increased the levels of TC, TG and ALB, and prominently restored the activities of SOD, GSH-Px, CAT and T-AOC in serum/liver homogenate against CCl4-induced injures.
26572391	1	5	theme	mycelia	365:371	arg1	polysaccharides					378:392	mycelia zinc polysaccharides	365:392	mycelia zinc polysaccharides (MZPSs)	365:400	This study was designed to investigate the physicochemical properties (molecular weights, bond types and monosaccharide compositions), antioxidant activities, and hepatoprotective effects on carbon tetrachloride (CCl4)-induced acute liver damage of mycelia zinc polysaccharides (MZPSs) and its major fractions (MZPS-1, -2 and -3) separated from Pleurotus djamor.
26572391	1	5	theme	mycelia	365:371	arg1	MZPSs					395:399	MZPSs	395:399	MZPSs	395:399	This study was designed to investigate the physicochemical properties (molecular weights, bond types and monosaccharide compositions), antioxidant activities, and hepatoprotective effects on carbon tetrachloride (CCl4)-induced acute liver damage of mycelia zinc polysaccharides (MZPSs) and its major fractions (MZPS-1, -2 and -3) separated from Pleurotus djamor.
26572391	3	6	from	GSH-Px	827:832	arg1	homogenate					864:873	serum/liver homogenate	852:873	serum/liver homogenate	852:873	For in vivo hepatoprotective activities, administration of MZPS-3 at 800 mg/kg significantly decreased the levels of AST, ALT, MDA and LPO, remarkably increased the levels of TC, TG and ALB, and prominently restored the activities of SOD, GSH-Px, CAT and T-AOC in serum/liver homogenate against CCl4-induced injures.
26572391	1	7	theme	hepatoprotective	279:294	arg1	effects					296:302	hepatoprotective effects	279:302	hepatoprotective effects on carbon tetrachloride (CCl4)-induced acute liver damage of mycelia zinc polysaccharides (MZPSs) and its major fractions (MZPS-1, -2 and -3) separated from Pleurotus djamor	279:476	This study was designed to investigate the physicochemical properties (molecular weights, bond types and monosaccharide compositions), antioxidant activities, and hepatoprotective effects on carbon tetrachloride (CCl4)-induced acute liver damage of mycelia zinc polysaccharides (MZPSs) and its major fractions (MZPS-1, -2 and -3) separated from Pleurotus djamor.
26572391	3	8	from	activities	808:817	arg1	homogenate					864:873	serum/liver homogenate	852:873	serum/liver homogenate	852:873	For in vivo hepatoprotective activities, administration of MZPS-3 at 800 mg/kg significantly decreased the levels of AST, ALT, MDA and LPO, remarkably increased the levels of TC, TG and ALB, and prominently restored the activities of SOD, GSH-Px, CAT and T-AOC in serum/liver homogenate against CCl4-induced injures.
26572391	1	9	theme	zinc	373:376	arg1	polysaccharides					378:392	mycelia zinc polysaccharides	365:392	mycelia zinc polysaccharides (MZPSs)	365:400	This study was designed to investigate the physicochemical properties (molecular weights, bond types and monosaccharide compositions), antioxidant activities, and hepatoprotective effects on carbon tetrachloride (CCl4)-induced acute liver damage of mycelia zinc polysaccharides (MZPSs) and its major fractions (MZPS-1, -2 and -3) separated from Pleurotus djamor.
26572391	1	9	theme	zinc	373:376	arg1	MZPSs					395:399	MZPSs	395:399	MZPSs	395:399	This study was designed to investigate the physicochemical properties (molecular weights, bond types and monosaccharide compositions), antioxidant activities, and hepatoprotective effects on carbon tetrachloride (CCl4)-induced acute liver damage of mycelia zinc polysaccharides (MZPSs) and its major fractions (MZPS-1, -2 and -3) separated from Pleurotus djamor.
26572391	3	10	theme	serum/liver	852:862	arg1	homogenate					864:873	serum/liver homogenate	852:873	serum/liver homogenate	852:873	For in vivo hepatoprotective activities, administration of MZPS-3 at 800 mg/kg significantly decreased the levels of AST, ALT, MDA and LPO, remarkably increased the levels of TC, TG and ALB, and prominently restored the activities of SOD, GSH-Px, CAT and T-AOC in serum/liver homogenate against CCl4-induced injures.
26572391	1	11	theme	polysaccharides	378:392	arg1	activities					263:272	antioxidant activities	251:272	antioxidant activities	251:272	This study was designed to investigate the physicochemical properties (molecular weights, bond types and monosaccharide compositions), antioxidant activities, and hepatoprotective effects on carbon tetrachloride (CCl4)-induced acute liver damage of mycelia zinc polysaccharides (MZPSs) and its major fractions (MZPS-1, -2 and -3) separated from Pleurotus djamor.
26572391	1	11	theme	polysaccharides	378:392	arg1	properties					175:184	the physicochemical properties	155:184	the physicochemical properties (molecular weights, bond types and monosaccharide compositions)	155:248	This study was designed to investigate the physicochemical properties (molecular weights, bond types and monosaccharide compositions), antioxidant activities, and hepatoprotective effects on carbon tetrachloride (CCl4)-induced acute liver damage of mycelia zinc polysaccharides (MZPSs) and its major fractions (MZPS-1, -2 and -3) separated from Pleurotus djamor.
26572391	1	11	theme	polysaccharides	378:392	arg1	effects					296:302	hepatoprotective effects	279:302	hepatoprotective effects on carbon tetrachloride (CCl4)-induced acute liver damage of mycelia zinc polysaccharides (MZPSs) and its major fractions (MZPS-1, -2 and -3) separated from Pleurotus djamor	279:476	This study was designed to investigate the physicochemical properties (molecular weights, bond types and monosaccharide compositions), antioxidant activities, and hepatoprotective effects on carbon tetrachloride (CCl4)-induced acute liver damage of mycelia zinc polysaccharides (MZPSs) and its major fractions (MZPS-1, -2 and -3) separated from Pleurotus djamor.
26572391	3	12	from	CAT	835:837	arg1	homogenate					864:873	serum/liver homogenate	852:873	serum/liver homogenate	852:873	For in vivo hepatoprotective activities, administration of MZPS-3 at 800 mg/kg significantly decreased the levels of AST, ALT, MDA and LPO, remarkably increased the levels of TC, TG and ALB, and prominently restored the activities of SOD, GSH-Px, CAT and T-AOC in serum/liver homogenate against CCl4-induced injures.
26572391	1	13	theme	molecular	187:195	arg1	weights					197:203	molecular weights	187:203	molecular weights	187:203	This study was designed to investigate the physicochemical properties (molecular weights, bond types and monosaccharide compositions), antioxidant activities, and hepatoprotective effects on carbon tetrachloride (CCl4)-induced acute liver damage of mycelia zinc polysaccharides (MZPSs) and its major fractions (MZPS-1, -2 and -3) separated from Pleurotus djamor.
26572391	3	14	from	mg/kg	661:665	arg1	administration					629:642	administration	629:642	administration of MZPS-3 at 800 mg/kg	629:665	For in vivo hepatoprotective activities, administration of MZPS-3 at 800 mg/kg significantly decreased the levels of AST, ALT, MDA and LPO, remarkably increased the levels of TC, TG and ALB, and prominently restored the activities of SOD, GSH-Px, CAT and T-AOC in serum/liver homogenate against CCl4-induced injures.
26572391	3	15	theme	T-AOC	843:847	arg1	activities					808:817	the activities	804:817	the activities of SOD, GSH-Px, CAT and T-AOC in serum/liver homogenate against CCl4-induced injures	804:902	For in vivo hepatoprotective activities, administration of MZPS-3 at 800 mg/kg significantly decreased the levels of AST, ALT, MDA and LPO, remarkably increased the levels of TC, TG and ALB, and prominently restored the activities of SOD, GSH-Px, CAT and T-AOC in serum/liver homogenate against CCl4-induced injures.
26572391	3	16	dep	in	592:593	arg1	vivo					595:598	vivo	595:598	vivo	595:598	For in vivo hepatoprotective activities, administration of MZPS-3 at 800 mg/kg significantly decreased the levels of AST, ALT, MDA and LPO, remarkably increased the levels of TC, TG and ALB, and prominently restored the activities of SOD, GSH-Px, CAT and T-AOC in serum/liver homogenate against CCl4-induced injures.
26572391	3	17	theme	MDA	715:717	arg1	levels					695:700	the levels	691:700	the levels of AST, ALT, MDA and LPO	691:725	For in vivo hepatoprotective activities, administration of MZPS-3 at 800 mg/kg significantly decreased the levels of AST, ALT, MDA and LPO, remarkably increased the levels of TC, TG and ALB, and prominently restored the activities of SOD, GSH-Px, CAT and T-AOC in serum/liver homogenate against CCl4-induced injures.
26572391	1	18	dep	properties	175:184	arg1	weights					197:203	molecular weights	187:203	molecular weights	187:203	This study was designed to investigate the physicochemical properties (molecular weights, bond types and monosaccharide compositions), antioxidant activities, and hepatoprotective effects on carbon tetrachloride (CCl4)-induced acute liver damage of mycelia zinc polysaccharides (MZPSs) and its major fractions (MZPS-1, -2 and -3) separated from Pleurotus djamor.
26572391	1	18	dep	properties	175:184	arg1	types					211:215	bond types	206:215	bond types	206:215	This study was designed to investigate the physicochemical properties (molecular weights, bond types and monosaccharide compositions), antioxidant activities, and hepatoprotective effects on carbon tetrachloride (CCl4)-induced acute liver damage of mycelia zinc polysaccharides (MZPSs) and its major fractions (MZPS-1, -2 and -3) separated from Pleurotus djamor.
26572391	1	18	dep	properties	175:184	arg1	compositions					236:247	monosaccharide compositions	221:247	monosaccharide compositions	221:247	This study was designed to investigate the physicochemical properties (molecular weights, bond types and monosaccharide compositions), antioxidant activities, and hepatoprotective effects on carbon tetrachloride (CCl4)-induced acute liver damage of mycelia zinc polysaccharides (MZPSs) and its major fractions (MZPS-1, -2 and -3) separated from Pleurotus djamor.
26572391	3	19	theme	LPO	723:725	arg1	levels					695:700	the levels	691:700	the levels of AST, ALT, MDA and LPO	691:725	For in vivo hepatoprotective activities, administration of MZPS-3 at 800 mg/kg significantly decreased the levels of AST, ALT, MDA and LPO, remarkably increased the levels of TC, TG and ALB, and prominently restored the activities of SOD, GSH-Px, CAT and T-AOC in serum/liver homogenate against CCl4-induced injures.
26572391	1	20	theme	Pleurotus	461:469	arg1	djamor					471:476	Pleurotus djamor	461:476	Pleurotus djamor	461:476	This study was designed to investigate the physicochemical properties (molecular weights, bond types and monosaccharide compositions), antioxidant activities, and hepatoprotective effects on carbon tetrachloride (CCl4)-induced acute liver damage of mycelia zinc polysaccharides (MZPSs) and its major fractions (MZPS-1, -2 and -3) separated from Pleurotus djamor.
26572391	4	21	theme	CCl4-induced	1065:1076	arg1	damage					1090:1095	the CCl4-induced acute liver damage	1061:1095	the CCl4-induced acute liver damage	1061:1095	Findings presented in this study clearly demonstrated that MZPSs, especially MZPS-3, might be suitable for functional foods and natural drugs in preventing the CCl4-induced acute liver damage.
26572391	1	22	theme	bond	206:209	arg1	types					211:215	bond types	206:215	bond types	206:215	This study was designed to investigate the physicochemical properties (molecular weights, bond types and monosaccharide compositions), antioxidant activities, and hepatoprotective effects on carbon tetrachloride (CCl4)-induced acute liver damage of mycelia zinc polysaccharides (MZPSs) and its major fractions (MZPS-1, -2 and -3) separated from Pleurotus djamor.
26572391	1	23	theme	carbon	307:312	arg1	tetrachloride					314:326	carbon tetrachloride	307:326	carbon tetrachloride (CCl4)-induced acute liver damage	307:360	This study was designed to investigate the physicochemical properties (molecular weights, bond types and monosaccharide compositions), antioxidant activities, and hepatoprotective effects on carbon tetrachloride (CCl4)-induced acute liver damage of mycelia zinc polysaccharides (MZPSs) and its major fractions (MZPS-1, -2 and -3) separated from Pleurotus djamor.
26572391	1	23	theme	carbon	307:312	arg1	CCl4					329:332	CCl4	329:332	CCl4	329:332	This study was designed to investigate the physicochemical properties (molecular weights, bond types and monosaccharide compositions), antioxidant activities, and hepatoprotective effects on carbon tetrachloride (CCl4)-induced acute liver damage of mycelia zinc polysaccharides (MZPSs) and its major fractions (MZPS-1, -2 and -3) separated from Pleurotus djamor.
26572391	3	24	from	SOD	822:824	arg1	homogenate					864:873	serum/liver homogenate	852:873	serum/liver homogenate	852:873	For in vivo hepatoprotective activities, administration of MZPS-3 at 800 mg/kg significantly decreased the levels of AST, ALT, MDA and LPO, remarkably increased the levels of TC, TG and ALB, and prominently restored the activities of SOD, GSH-Px, CAT and T-AOC in serum/liver homogenate against CCl4-induced injures.
26572391	1	25	theme	tetrachloride	314:326	arg1	damage					355:360	carbon tetrachloride (CCl4)-induced acute liver damage	307:360	carbon tetrachloride (CCl4)-induced acute liver damage	307:360	This study was designed to investigate the physicochemical properties (molecular weights, bond types and monosaccharide compositions), antioxidant activities, and hepatoprotective effects on carbon tetrachloride (CCl4)-induced acute liver damage of mycelia zinc polysaccharides (MZPSs) and its major fractions (MZPS-1, -2 and -3) separated from Pleurotus djamor.
26572391	1	26	from	effects	296:302	arg1	damage					355:360	carbon tetrachloride (CCl4)-induced acute liver damage	307:360	carbon tetrachloride (CCl4)-induced acute liver damage	307:360	This study was designed to investigate the physicochemical properties (molecular weights, bond types and monosaccharide compositions), antioxidant activities, and hepatoprotective effects on carbon tetrachloride (CCl4)-induced acute liver damage of mycelia zinc polysaccharides (MZPSs) and its major fractions (MZPS-1, -2 and -3) separated from Pleurotus djamor.
26572391	4	27	theme	acute	1078:1082	arg1	damage					1090:1095	the CCl4-induced acute liver damage	1061:1095	the CCl4-induced acute liver damage	1061:1095	Findings presented in this study clearly demonstrated that MZPSs, especially MZPS-3, might be suitable for functional foods and natural drugs in preventing the CCl4-induced acute liver damage.
26572391	4	28	theme	liver	1084:1088	arg1	damage					1090:1095	the CCl4-induced acute liver damage	1061:1095	the CCl4-induced acute liver damage	1061:1095	Findings presented in this study clearly demonstrated that MZPSs, especially MZPS-3, might be suitable for functional foods and natural drugs in preventing the CCl4-induced acute liver damage.
26572391	2	29	theme	strong	531:536	arg1	activities					550:559	relatively strong antioxidant activities	520:559	relatively strong antioxidant activities	520:559	In vitro assays, the MZPS-3 demonstrated relatively strong antioxidant activities in dose-dependent manners.
26572391	3	30	theme	CCl4-induced	883:894	arg1	injures					896:902	CCl4-induced injures	883:902	CCl4-induced injures	883:902	For in vivo hepatoprotective activities, administration of MZPS-3 at 800 mg/kg significantly decreased the levels of AST, ALT, MDA and LPO, remarkably increased the levels of TC, TG and ALB, and prominently restored the activities of SOD, GSH-Px, CAT and T-AOC in serum/liver homogenate against CCl4-induced injures.
26572391	1	31	theme	monosaccharide	221:234	arg1	compositions					236:247	monosaccharide compositions	221:247	monosaccharide compositions	221:247	This study was designed to investigate the physicochemical properties (molecular weights, bond types and monosaccharide compositions), antioxidant activities, and hepatoprotective effects on carbon tetrachloride (CCl4)-induced acute liver damage of mycelia zinc polysaccharides (MZPSs) and its major fractions (MZPS-1, -2 and -3) separated from Pleurotus djamor.
26572391	0	32	theme	hepatoprotective	35:50	arg1	activities					52:61	hepatoprotective activities	35:61	hepatoprotective activities	35:61	Purification, characterization and hepatoprotective activities of mycelia zinc polysaccharides by Pleurotus djamor.
26572391	3	33	theme	TC	763:764	arg1	levels					753:758	the levels	749:758	the levels of TC, TG and ALB	749:776	For in vivo hepatoprotective activities, administration of MZPS-3 at 800 mg/kg significantly decreased the levels of AST, ALT, MDA and LPO, remarkably increased the levels of TC, TG and ALB, and prominently restored the activities of SOD, GSH-Px, CAT and T-AOC in serum/liver homogenate against CCl4-induced injures.
26572391	3	34	theme	MZPS-3	647:652	arg1	administration					629:642	administration	629:642	administration of MZPS-3 at 800 mg/kg	629:665	For in vivo hepatoprotective activities, administration of MZPS-3 at 800 mg/kg significantly decreased the levels of AST, ALT, MDA and LPO, remarkably increased the levels of TC, TG and ALB, and prominently restored the activities of SOD, GSH-Px, CAT and T-AOC in serum/liver homogenate against CCl4-induced injures.
26572391	3	35	theme	TG	767:768	arg1	levels					753:758	the levels	749:758	the levels of TC, TG and ALB	749:776	For in vivo hepatoprotective activities, administration of MZPS-3 at 800 mg/kg significantly decreased the levels of AST, ALT, MDA and LPO, remarkably increased the levels of TC, TG and ALB, and prominently restored the activities of SOD, GSH-Px, CAT and T-AOC in serum/liver homogenate against CCl4-induced injures.
26572391	1	36	dep	polysaccharides	378:392	arg1	MZPS-1					427:432	MZPS-1	427:432	MZPS-1	427:432	This study was designed to investigate the physicochemical properties (molecular weights, bond types and monosaccharide compositions), antioxidant activities, and hepatoprotective effects on carbon tetrachloride (CCl4)-induced acute liver damage of mycelia zinc polysaccharides (MZPSs) and its major fractions (MZPS-1, -2 and -3) separated from Pleurotus djamor.
26572391	0	37	theme	mycelia	66:72	arg1	polysaccharides					79:93	mycelia zinc polysaccharides	66:93	mycelia zinc polysaccharides	66:93	Purification, characterization and hepatoprotective activities of mycelia zinc polysaccharides by Pleurotus djamor.
26572391	2	38	dep	In	479:480	arg1	vitro					482:486	vitro	482:486	vitro	482:486	In vitro assays, the MZPS-3 demonstrated relatively strong antioxidant activities in dose-dependent manners.
26572391	1	39	theme	major	410:414	arg1	fractions					416:424	its major fractions	406:424	its major fractions	406:424	This study was designed to investigate the physicochemical properties (molecular weights, bond types and monosaccharide compositions), antioxidant activities, and hepatoprotective effects on carbon tetrachloride (CCl4)-induced acute liver damage of mycelia zinc polysaccharides (MZPSs) and its major fractions (MZPS-1, -2 and -3) separated from Pleurotus djamor.
26572391	3	40	theme	AST	705:707	arg1	levels					695:700	the levels	691:700	the levels of AST, ALT, MDA and LPO	691:725	For in vivo hepatoprotective activities, administration of MZPS-3 at 800 mg/kg significantly decreased the levels of AST, ALT, MDA and LPO, remarkably increased the levels of TC, TG and ALB, and prominently restored the activities of SOD, GSH-Px, CAT and T-AOC in serum/liver homogenate against CCl4-induced injures.
26572391	1	41	theme	-induced	334:341	arg1	damage					355:360	carbon tetrachloride (CCl4)-induced acute liver damage	307:360	carbon tetrachloride (CCl4)-induced acute liver damage	307:360	This study was designed to investigate the physicochemical properties (molecular weights, bond types and monosaccharide compositions), antioxidant activities, and hepatoprotective effects on carbon tetrachloride (CCl4)-induced acute liver damage of mycelia zinc polysaccharides (MZPSs) and its major fractions (MZPS-1, -2 and -3) separated from Pleurotus djamor.
26572391	1	42	from	activities	263:272	arg1	damage					355:360	carbon tetrachloride (CCl4)-induced acute liver damage	307:360	carbon tetrachloride (CCl4)-induced acute liver damage	307:360	This study was designed to investigate the physicochemical properties (molecular weights, bond types and monosaccharide compositions), antioxidant activities, and hepatoprotective effects on carbon tetrachloride (CCl4)-induced acute liver damage of mycelia zinc polysaccharides (MZPSs) and its major fractions (MZPS-1, -2 and -3) separated from Pleurotus djamor.
26572391	1	43	theme	fractions	416:424	arg1	activities					263:272	antioxidant activities	251:272	antioxidant activities	251:272	This study was designed to investigate the physicochemical properties (molecular weights, bond types and monosaccharide compositions), antioxidant activities, and hepatoprotective effects on carbon tetrachloride (CCl4)-induced acute liver damage of mycelia zinc polysaccharides (MZPSs) and its major fractions (MZPS-1, -2 and -3) separated from Pleurotus djamor.
26572391	1	43	theme	fractions	416:424	arg1	properties					175:184	the physicochemical properties	155:184	the physicochemical properties (molecular weights, bond types and monosaccharide compositions)	155:248	This study was designed to investigate the physicochemical properties (molecular weights, bond types and monosaccharide compositions), antioxidant activities, and hepatoprotective effects on carbon tetrachloride (CCl4)-induced acute liver damage of mycelia zinc polysaccharides (MZPSs) and its major fractions (MZPS-1, -2 and -3) separated from Pleurotus djamor.
26572391	1	43	theme	fractions	416:424	arg1	effects					296:302	hepatoprotective effects	279:302	hepatoprotective effects on carbon tetrachloride (CCl4)-induced acute liver damage of mycelia zinc polysaccharides (MZPSs) and its major fractions (MZPS-1, -2 and -3) separated from Pleurotus djamor	279:476	This study was designed to investigate the physicochemical properties (molecular weights, bond types and monosaccharide compositions), antioxidant activities, and hepatoprotective effects on carbon tetrachloride (CCl4)-induced acute liver damage of mycelia zinc polysaccharides (MZPSs) and its major fractions (MZPS-1, -2 and -3) separated from Pleurotus djamor.
26572391	3	44	theme	in	592:593	arg1	activities					617:626	in vivo hepatoprotective activities	592:626	in vivo hepatoprotective activities	592:626	For in vivo hepatoprotective activities, administration of MZPS-3 at 800 mg/kg significantly decreased the levels of AST, ALT, MDA and LPO, remarkably increased the levels of TC, TG and ALB, and prominently restored the activities of SOD, GSH-Px, CAT and T-AOC in serum/liver homogenate against CCl4-induced injures.
26572391	3	45	theme	SOD	822:824	arg1	activities					808:817	the activities	804:817	the activities of SOD, GSH-Px, CAT and T-AOC in serum/liver homogenate against CCl4-induced injures	804:902	For in vivo hepatoprotective activities, administration of MZPS-3 at 800 mg/kg significantly decreased the levels of AST, ALT, MDA and LPO, remarkably increased the levels of TC, TG and ALB, and prominently restored the activities of SOD, GSH-Px, CAT and T-AOC in serum/liver homogenate against CCl4-induced injures.
26572391	1	46	theme	acute	343:347	arg1	damage					355:360	carbon tetrachloride (CCl4)-induced acute liver damage	307:360	carbon tetrachloride (CCl4)-induced acute liver damage	307:360	This study was designed to investigate the physicochemical properties (molecular weights, bond types and monosaccharide compositions), antioxidant activities, and hepatoprotective effects on carbon tetrachloride (CCl4)-induced acute liver damage of mycelia zinc polysaccharides (MZPSs) and its major fractions (MZPS-1, -2 and -3) separated from Pleurotus djamor.
26572391	2	47	theme	dose-dependent	564:577	arg1	manners					579:585	dose-dependent manners	564:585	dose-dependent manners	564:585	In vitro assays, the MZPS-3 demonstrated relatively strong antioxidant activities in dose-dependent manners.
26572391	3	48	from	T-AOC	843:847	arg1	homogenate					864:873	serum/liver homogenate	852:873	serum/liver homogenate	852:873	For in vivo hepatoprotective activities, administration of MZPS-3 at 800 mg/kg significantly decreased the levels of AST, ALT, MDA and LPO, remarkably increased the levels of TC, TG and ALB, and prominently restored the activities of SOD, GSH-Px, CAT and T-AOC in serum/liver homogenate against CCl4-induced injures.
26572391	3	49	theme	hepatoprotective	600:615	arg1	activities					617:626	in vivo hepatoprotective activities	592:626	in vivo hepatoprotective activities	592:626	For in vivo hepatoprotective activities, administration of MZPS-3 at 800 mg/kg significantly decreased the levels of AST, ALT, MDA and LPO, remarkably increased the levels of TC, TG and ALB, and prominently restored the activities of SOD, GSH-Px, CAT and T-AOC in serum/liver homogenate against CCl4-induced injures.
26572391	4	50	theme	natural	1033:1039	arg1	drugs					1041:1045	natural drugs	1033:1045	natural drugs	1033:1045	Findings presented in this study clearly demonstrated that MZPSs, especially MZPS-3, might be suitable for functional foods and natural drugs in preventing the CCl4-induced acute liver damage.
26572391	3	51	theme	ALB	774:776	arg1	levels					753:758	the levels	749:758	the levels of TC, TG and ALB	749:776	For in vivo hepatoprotective activities, administration of MZPS-3 at 800 mg/kg significantly decreased the levels of AST, ALT, MDA and LPO, remarkably increased the levels of TC, TG and ALB, and prominently restored the activities of SOD, GSH-Px, CAT and T-AOC in serum/liver homogenate against CCl4-induced injures.
26572391	0	52	theme	polysaccharides	79:93	arg1	characterization					14:29	characterization	14:29	characterization	14:29	Purification, characterization and hepatoprotective activities of mycelia zinc polysaccharides by Pleurotus djamor.
26572391	0	52	theme	polysaccharides	79:93	arg1	activities					52:61	hepatoprotective activities	35:61	hepatoprotective activities	35:61	Purification, characterization and hepatoprotective activities of mycelia zinc polysaccharides by Pleurotus djamor.
26572391	0	52	theme	polysaccharides	79:93	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification, characterization and hepatoprotective activities of mycelia zinc polysaccharides by Pleurotus djamor.
26572391	4	53	theme	functional	1012:1021	arg1	foods					1023:1027	functional foods	1012:1027	functional foods	1012:1027	Findings presented in this study clearly demonstrated that MZPSs, especially MZPS-3, might be suitable for functional foods and natural drugs in preventing the CCl4-induced acute liver damage.
26572391	1	54	theme	antioxidant	251:261	arg1	activities					263:272	antioxidant activities	251:272	antioxidant activities	251:272	This study was designed to investigate the physicochemical properties (molecular weights, bond types and monosaccharide compositions), antioxidant activities, and hepatoprotective effects on carbon tetrachloride (CCl4)-induced acute liver damage of mycelia zinc polysaccharides (MZPSs) and its major fractions (MZPS-1, -2 and -3) separated from Pleurotus djamor.
26572391	0	55	theme	zinc	74:77	arg1	polysaccharides					79:93	mycelia zinc polysaccharides	66:93	mycelia zinc polysaccharides	66:93	Purification, characterization and hepatoprotective activities of mycelia zinc polysaccharides by Pleurotus djamor.
26572391	1	56	theme	liver	349:353	arg1	damage					355:360	carbon tetrachloride (CCl4)-induced acute liver damage	307:360	carbon tetrachloride (CCl4)-induced acute liver damage	307:360	This study was designed to investigate the physicochemical properties (molecular weights, bond types and monosaccharide compositions), antioxidant activities, and hepatoprotective effects on carbon tetrachloride (CCl4)-induced acute liver damage of mycelia zinc polysaccharides (MZPSs) and its major fractions (MZPS-1, -2 and -3) separated from Pleurotus djamor.
26572391	3	57	from	homogenate	864:873	arg1	activities					808:817	the activities	804:817	the activities of SOD, GSH-Px, CAT and T-AOC in serum/liver homogenate against CCl4-induced injures	804:902	For in vivo hepatoprotective activities, administration of MZPS-3 at 800 mg/kg significantly decreased the levels of AST, ALT, MDA and LPO, remarkably increased the levels of TC, TG and ALB, and prominently restored the activities of SOD, GSH-Px, CAT and T-AOC in serum/liver homogenate against CCl4-induced injures.
26572391	3	58	theme	ALT	710:712	arg1	levels					695:700	the levels	691:700	the levels of AST, ALT, MDA and LPO	691:725	For in vivo hepatoprotective activities, administration of MZPS-3 at 800 mg/kg significantly decreased the levels of AST, ALT, MDA and LPO, remarkably increased the levels of TC, TG and ALB, and prominently restored the activities of SOD, GSH-Px, CAT and T-AOC in serum/liver homogenate against CCl4-induced injures.
26572391	1	59	theme	physicochemical	159:173	arg1	properties					175:184	the physicochemical properties	155:184	the physicochemical properties (molecular weights, bond types and monosaccharide compositions)	155:248	This study was designed to investigate the physicochemical properties (molecular weights, bond types and monosaccharide compositions), antioxidant activities, and hepatoprotective effects on carbon tetrachloride (CCl4)-induced acute liver damage of mycelia zinc polysaccharides (MZPSs) and its major fractions (MZPS-1, -2 and -3) separated from Pleurotus djamor.
26572391	3	60	theme	CAT	835:837	arg1	activities					808:817	the activities	804:817	the activities of SOD, GSH-Px, CAT and T-AOC in serum/liver homogenate against CCl4-induced injures	804:902	For in vivo hepatoprotective activities, administration of MZPS-3 at 800 mg/kg significantly decreased the levels of AST, ALT, MDA and LPO, remarkably increased the levels of TC, TG and ALB, and prominently restored the activities of SOD, GSH-Px, CAT and T-AOC in serum/liver homogenate against CCl4-induced injures.
27923477	7	0	theme	mixtures	1260:1267	arg1	separation					1234:1243	improved separation	1225:1243	improved separation of complicated mixtures of acidic disaccharides and oligosaccharides	1225:1312	A maltose modified hydrophilic interaction column offers improved separation of complicated mixtures of acidic disaccharides and oligosaccharides.
27923477	6	1	theme	sensitive	909:917	arg1	approach					937:944	A sensitive and comprehensive approach	907:944	A sensitive and comprehensive approach	907:944	A sensitive and comprehensive approach is needed for detailed investigation of the structure of LMWHs prepared through nitrous acid depolymerization, especially their characteristic saturated non-reducing end (NRE) and 2,5-anhydro-d-mannitol reducing end (RE).
27923477	4	2	from	information	775:785	arg1	LMWHs					802:806	LMWHs	802:806	LMWHs	802:806	Compositional analysis of their basic building blocks is an effective way to provide structural information on heparin and LMWHs.
27923477	4	2	from	information	775:785	arg1	heparin					790:796	heparin	790:796	heparin	790:796	Compositional analysis of their basic building blocks is an effective way to provide structural information on heparin and LMWHs.
27923477	6	3	theme	LMWHs	1003:1007	arg1	structure					990:998	the structure	986:998	the structure of LMWHs prepared through nitrous acid depolymerization, especially their characteristic saturated non-reducing end (NRE) and 2,5-anhydro-d-mannitol reducing end (RE)	986:1165	A sensitive and comprehensive approach is needed for detailed investigation of the structure of LMWHs prepared through nitrous acid depolymerization, especially their characteristic saturated non-reducing end (NRE) and 2,5-anhydro-d-mannitol reducing end (RE).
27923477	4	4	theme	Compositional	679:691	arg1	way					749:751	an effective way	736:751	an effective way to provide structural information on heparin and LMWHs	736:806	Compositional analysis of their basic building blocks is an effective way to provide structural information on heparin and LMWHs.
27923477	4	4	theme	Compositional	679:691	arg1	analysis					693:700	Compositional analysis	679:700	Compositional analysis of their basic building blocks	679:731	Compositional analysis of their basic building blocks is an effective way to provide structural information on heparin and LMWHs.
27923477	3	5	theme	nitrous	614:620	arg1	depolymerization					627:642	nitrous acid depolymerization	614:642	nitrous acid depolymerization followed by borohydride reduction	614:676	Enoxaparin is prepared by benzyl esterification and alkaline depolymerization, while dalteparin and nadroparin are prepared through nitrous acid depolymerization followed by borohydride reduction.
27923477	6	6	theme	structure	990:998	arg1	investigation					969:981	detailed investigation	960:981	detailed investigation of the structure of LMWHs prepared through nitrous acid depolymerization, especially their characteristic saturated non-reducing end (NRE) and 2,5-anhydro-d-mannitol reducing end (RE)	960:1165	A sensitive and comprehensive approach is needed for detailed investigation of the structure of LMWHs prepared through nitrous acid depolymerization, especially their characteristic saturated non-reducing end (NRE) and 2,5-anhydro-d-mannitol reducing end (RE).
27923477	8	7	theme	high-resolution	1384:1398	arg1	MS					1426:1427	MS	1426:1427	MS	1426:1427	A total of 36 basic building blocks were unambiguously identified by high-resolution tandem mass spectrometry (MS).
27923477	8	7	theme	high-resolution	1384:1398	arg1	spectrometry					1412:1423	high-resolution tandem mass spectrometry	1384:1423	high-resolution tandem mass spectrometry (MS)	1384:1428	A total of 36 basic building blocks were unambiguously identified by high-resolution tandem mass spectrometry (MS).
27923477	7	8	theme	improved	1225:1232	arg1	separation					1234:1243	improved separation	1225:1243	improved separation of complicated mixtures of acidic disaccharides and oligosaccharides	1225:1312	A maltose modified hydrophilic interaction column offers improved separation of complicated mixtures of acidic disaccharides and oligosaccharides.
27923477	1	9	theme	molecular	212:220	arg1	drugs					274:278	important anticoagulant drugs	250:278	important anticoagulant drugs that are prepared through depolymerization of unfractionated heparin	250:347	Low molecular weight heparins (LMWHs) are important anticoagulant drugs that are prepared through depolymerization of unfractionated heparin.
27923477	1	9	theme	molecular	212:220	arg1	LMWHs					239:243	LMWHs	239:243	LMWHs	239:243	Low molecular weight heparins (LMWHs) are important anticoagulant drugs that are prepared through depolymerization of unfractionated heparin.
27923477	1	9	theme	molecular	212:220	arg1	heparins					229:236	Low molecular weight heparins	208:236	Low molecular weight heparins (LMWHs)	208:244	Low molecular weight heparins (LMWHs) are important anticoagulant drugs that are prepared through depolymerization of unfractionated heparin.
27923477	8	10	theme	mass	1407:1410	arg1	MS					1426:1427	MS	1426:1427	MS	1426:1427	A total of 36 basic building blocks were unambiguously identified by high-resolution tandem mass spectrometry (MS).
27923477	8	10	theme	mass	1407:1410	arg1	spectrometry					1412:1423	high-resolution tandem mass spectrometry	1384:1423	high-resolution tandem mass spectrometry (MS)	1384:1428	A total of 36 basic building blocks were unambiguously identified by high-resolution tandem mass spectrometry (MS).
27923477	10	11	theme	materials	1751:1759	arg1	structure					1721:1729	the structure	1717:1729	the structure of heparin starting materials, cleavage sites in the depolymerization reaction	1717:1808	Each group of building blocks revealed different aspects of the properties of LMWHs, such as functional motifs required for anticoagulant activity, the structure of heparin starting materials, cleavage sites in the depolymerization reaction, and undesired structural modifications resulting from side reactions.
27923477	10	11	theme	materials	1751:1759	arg1	activity					1707:1714	anticoagulant activity	1693:1714	anticoagulant activity	1693:1714	Each group of building blocks revealed different aspects of the properties of LMWHs, such as functional motifs required for anticoagulant activity, the structure of heparin starting materials, cleavage sites in the depolymerization reaction, and undesired structural modifications resulting from side reactions.
27923477	10	11	theme	materials	1751:1759	arg1	modifications					1836:1848	undesired structural modifications	1815:1848	undesired structural modifications resulting from side reactions	1815:1878	Each group of building blocks revealed different aspects of the properties of LMWHs, such as functional motifs required for anticoagulant activity, the structure of heparin starting materials, cleavage sites in the depolymerization reaction, and undesired structural modifications resulting from side reactions.
27923477	8	12	theme	building	1335:1342	arg1	blocks					1344:1349	36 basic building blocks	1326:1349	36 basic building blocks	1326:1349	A total of 36 basic building blocks were unambiguously identified by high-resolution tandem mass spectrometry (MS).
27923477	6	13	theme	saturated	1089:1097	arg1	NRE					1117:1119	NRE	1117:1119	NRE	1117:1119	A sensitive and comprehensive approach is needed for detailed investigation of the structure of LMWHs prepared through nitrous acid depolymerization, especially their characteristic saturated non-reducing end (NRE) and 2,5-anhydro-d-mannitol reducing end (RE).
27923477	6	13	theme	saturated	1089:1097	arg1	end					1112:1114	their characteristic saturated non-reducing end	1068:1114	their characteristic saturated non-reducing end (NRE)	1068:1120	A sensitive and comprehensive approach is needed for detailed investigation of the structure of LMWHs prepared through nitrous acid depolymerization, especially their characteristic saturated non-reducing end (NRE) and 2,5-anhydro-d-mannitol reducing end (RE).
27923477	6	13	theme	saturated	1089:1097	arg1	LMWHs					1003:1007	LMWHs	1003:1007	LMWHs prepared through nitrous acid depolymerization	1003:1054	A sensitive and comprehensive approach is needed for detailed investigation of the structure of LMWHs prepared through nitrous acid depolymerization, especially their characteristic saturated non-reducing end (NRE) and 2,5-anhydro-d-mannitol reducing end (RE).
27923477	4	14	theme	blocks	726:731	arg1	way					749:751	an effective way	736:751	an effective way to provide structural information on heparin and LMWHs	736:806	Compositional analysis of their basic building blocks is an effective way to provide structural information on heparin and LMWHs.
27923477	4	14	theme	blocks	726:731	arg1	analysis					693:700	Compositional analysis	679:700	Compositional analysis of their basic building blocks	679:731	Compositional analysis of their basic building blocks is an effective way to provide structural information on heparin and LMWHs.
27923477	10	15	theme	heparin	1734:1740	arg1	materials					1751:1759	heparin starting materials	1734:1759	heparin starting materials	1734:1759	Each group of building blocks revealed different aspects of the properties of LMWHs, such as functional motifs required for anticoagulant activity, the structure of heparin starting materials, cleavage sites in the depolymerization reaction, and undesired structural modifications resulting from side reactions.
27923477	10	15	theme	heparin	1734:1740	arg1	sites					1771:1775	cleavage sites	1762:1775	cleavage sites in the depolymerization reaction	1762:1808	Each group of building blocks revealed different aspects of the properties of LMWHs, such as functional motifs required for anticoagulant activity, the structure of heparin starting materials, cleavage sites in the depolymerization reaction, and undesired structural modifications resulting from side reactions.
27923477	7	16	theme	hydrophilic	1187:1197	arg1	column					1211:1216	A maltose modified hydrophilic interaction column	1168:1216	A maltose modified hydrophilic interaction column	1168:1216	A maltose modified hydrophilic interaction column offers improved separation of complicated mixtures of acidic disaccharides and oligosaccharides.
27923477	10	17	theme	depolymerization	1784:1799	arg1	reaction					1801:1808	the depolymerization reaction	1780:1808	the depolymerization reaction	1780:1808	Each group of building blocks revealed different aspects of the properties of LMWHs, such as functional motifs required for anticoagulant activity, the structure of heparin starting materials, cleavage sites in the depolymerization reaction, and undesired structural modifications resulting from side reactions.
27923477	0	18	theme	reaction	48:55	arg1	spectrometry					73:84	Hydrophilic interaction chromatography-multiple reaction monitoring mass spectrometry	0:84	Hydrophilic interaction chromatography-multiple reaction monitoring mass spectrometry method for basic building	0:110	Hydrophilic interaction chromatography-multiple reaction monitoring mass spectrometry method for basic building block analysis of low molecular weight heparins prepared through nitrous acid depolymerization.
27923477	10	19	theme	cleavage	1762:1769	arg1	materials					1751:1759	heparin starting materials	1734:1759	heparin starting materials	1734:1759	Each group of building blocks revealed different aspects of the properties of LMWHs, such as functional motifs required for anticoagulant activity, the structure of heparin starting materials, cleavage sites in the depolymerization reaction, and undesired structural modifications resulting from side reactions.
27923477	10	19	theme	cleavage	1762:1769	arg1	sites					1771:1775	cleavage sites	1762:1775	cleavage sites in the depolymerization reaction	1762:1808	Each group of building blocks revealed different aspects of the properties of LMWHs, such as functional motifs required for anticoagulant activity, the structure of heparin starting materials, cleavage sites in the depolymerization reaction, and undesired structural modifications resulting from side reactions.
27923477	0	20	theme	nitrous	177:183	arg1	depolymerization					190:205	nitrous acid depolymerization	177:205	nitrous acid depolymerization	177:205	Hydrophilic interaction chromatography-multiple reaction monitoring mass spectrometry method for basic building block analysis of low molecular weight heparins prepared through nitrous acid depolymerization.
27923477	6	21	theme	acid	1034:1037	arg1	depolymerization					1039:1054	nitrous acid depolymerization	1026:1054	nitrous acid depolymerization	1026:1054	A sensitive and comprehensive approach is needed for detailed investigation of the structure of LMWHs prepared through nitrous acid depolymerization, especially their characteristic saturated non-reducing end (NRE) and 2,5-anhydro-d-mannitol reducing end (RE).
27923477	6	22	theme	reducing	1149:1156	arg1	RE					1163:1164	RE	1163:1164	RE	1163:1164	A sensitive and comprehensive approach is needed for detailed investigation of the structure of LMWHs prepared through nitrous acid depolymerization, especially their characteristic saturated non-reducing end (NRE) and 2,5-anhydro-d-mannitol reducing end (RE).
27923477	6	22	theme	reducing	1149:1156	arg1	LMWHs					1003:1007	LMWHs	1003:1007	LMWHs prepared through nitrous acid depolymerization	1003:1054	A sensitive and comprehensive approach is needed for detailed investigation of the structure of LMWHs prepared through nitrous acid depolymerization, especially their characteristic saturated non-reducing end (NRE) and 2,5-anhydro-d-mannitol reducing end (RE).
27923477	6	22	theme	reducing	1149:1156	arg1	end					1158:1160	2,5-anhydro-d-mannitol reducing end	1126:1160	2,5-anhydro-d-mannitol reducing end (RE)	1126:1165	A sensitive and comprehensive approach is needed for detailed investigation of the structure of LMWHs prepared through nitrous acid depolymerization, especially their characteristic saturated non-reducing end (NRE) and 2,5-anhydro-d-mannitol reducing end (RE).
27923477	3	23	theme	benzyl	508:513	arg1	esterification					515:528	benzyl esterification	508:528	benzyl esterification	508:528	Enoxaparin is prepared by benzyl esterification and alkaline depolymerization, while dalteparin and nadroparin are prepared through nitrous acid depolymerization followed by borohydride reduction.
27923477	9	24	theme	reaction	1440:1447	arg1	MRM					1461:1463	MRM	1461:1463	MRM	1461:1463	Multiple reaction monitoring (MRM) MS/MS quantification was developed and validated in the analysis of dalteparin and nadroparin samples.
27923477	9	24	theme	reaction	1440:1447	arg1	monitoring					1449:1458	Multiple reaction monitoring	1431:1458	Multiple reaction monitoring (MRM) MS/MS quantification	1431:1485	Multiple reaction monitoring (MRM) MS/MS quantification was developed and validated in the analysis of dalteparin and nadroparin samples.
27923477	10	25	theme	building	1583:1590	arg1	blocks					1592:1597	building blocks	1583:1597	building blocks	1583:1597	Each group of building blocks revealed different aspects of the properties of LMWHs, such as functional motifs required for anticoagulant activity, the structure of heparin starting materials, cleavage sites in the depolymerization reaction, and undesired structural modifications resulting from side reactions.
27923477	5	26	theme	most	818:821	arg1	methods					854:860	most current compositional analysis methods	818:860	most current compositional analysis methods	818:860	However, most current compositional analysis methods have been limited to heparin and enoxaparin.
27923477	0	27	theme	low	130:132	arg1	heparins					151:158	low molecular weight heparins	130:158	low molecular weight heparins prepared through nitrous acid depolymerization	130:205	Hydrophilic interaction chromatography-multiple reaction monitoring mass spectrometry method for basic building block analysis of low molecular weight heparins prepared through nitrous acid depolymerization.
27923477	0	28	theme	mass	68:71	arg1	spectrometry					73:84	Hydrophilic interaction chromatography-multiple reaction monitoring mass spectrometry	0:84	Hydrophilic interaction chromatography-multiple reaction monitoring mass spectrometry method for basic building	0:110	Hydrophilic interaction chromatography-multiple reaction monitoring mass spectrometry method for basic building block analysis of low molecular weight heparins prepared through nitrous acid depolymerization.
27923477	0	29	theme	basic	97:101	arg1	building					103:110	basic building	97:110	basic building	97:110	Hydrophilic interaction chromatography-multiple reaction monitoring mass spectrometry method for basic building block analysis of low molecular weight heparins prepared through nitrous acid depolymerization.
27923477	0	30	theme	Hydrophilic	0:10	arg1	spectrometry					73:84	Hydrophilic interaction chromatography-multiple reaction monitoring mass spectrometry	0:84	Hydrophilic interaction chromatography-multiple reaction monitoring mass spectrometry method for basic building	0:110	Hydrophilic interaction chromatography-multiple reaction monitoring mass spectrometry method for basic building block analysis of low molecular weight heparins prepared through nitrous acid depolymerization.
27923477	2	31	theme	different	455:463	arg1	classifications					465:479	different classifications	455:479	different classifications	455:479	Based on the types of processing reactions and the structures of the products, LMWHs can be divided into different classifications.
27923477	0	32	theme	chromatography-multiple	24:46	arg1	spectrometry					73:84	Hydrophilic interaction chromatography-multiple reaction monitoring mass spectrometry	0:84	Hydrophilic interaction chromatography-multiple reaction monitoring mass spectrometry method for basic building	0:110	Hydrophilic interaction chromatography-multiple reaction monitoring mass spectrometry method for basic building block analysis of low molecular weight heparins prepared through nitrous acid depolymerization.
27923477	1	33	theme	heparin	341:347	arg1	depolymerization					306:321	depolymerization	306:321	depolymerization of unfractionated heparin	306:347	Low molecular weight heparins (LMWHs) are important anticoagulant drugs that are prepared through depolymerization of unfractionated heparin.
27923477	10	34	theme	structural	1825:1834	arg1	modifications					1836:1848	undesired structural modifications	1815:1848	undesired structural modifications resulting from side reactions	1815:1878	Each group of building blocks revealed different aspects of the properties of LMWHs, such as functional motifs required for anticoagulant activity, the structure of heparin starting materials, cleavage sites in the depolymerization reaction, and undesired structural modifications resulting from side reactions.
27923477	0	35	theme	monitoring	57:66	arg1	spectrometry					73:84	Hydrophilic interaction chromatography-multiple reaction monitoring mass spectrometry	0:84	Hydrophilic interaction chromatography-multiple reaction monitoring mass spectrometry method for basic building	0:110	Hydrophilic interaction chromatography-multiple reaction monitoring mass spectrometry method for basic building block analysis of low molecular weight heparins prepared through nitrous acid depolymerization.
27923477	0	36	theme	spectrometry	73:84	arg1	method					86:91	Hydrophilic interaction chromatography-multiple reaction monitoring mass spectrometry method	0:91	Hydrophilic interaction chromatography-multiple reaction monitoring mass spectrometry method for basic building	0:110	Hydrophilic interaction chromatography-multiple reaction monitoring mass spectrometry method for basic building block analysis of low molecular weight heparins prepared through nitrous acid depolymerization.
27923477	2	37	theme	processing	372:381	arg1	reactions					383:391	processing reactions	372:391	processing reactions	372:391	Based on the types of processing reactions and the structures of the products, LMWHs can be divided into different classifications.
27923477	1	38	theme	important	250:258	arg1	drugs					274:278	important anticoagulant drugs	250:278	important anticoagulant drugs that are prepared through depolymerization of unfractionated heparin	250:347	Low molecular weight heparins (LMWHs) are important anticoagulant drugs that are prepared through depolymerization of unfractionated heparin.
27923477	1	38	theme	important	250:258	arg1	heparins					229:236	Low molecular weight heparins	208:236	Low molecular weight heparins (LMWHs)	208:244	Low molecular weight heparins (LMWHs) are important anticoagulant drugs that are prepared through depolymerization of unfractionated heparin.
27923477	6	39	theme	comprehensive	923:935	arg1	approach					937:944	A sensitive and comprehensive approach	907:944	A sensitive and comprehensive approach	907:944	A sensitive and comprehensive approach is needed for detailed investigation of the structure of LMWHs prepared through nitrous acid depolymerization, especially their characteristic saturated non-reducing end (NRE) and 2,5-anhydro-d-mannitol reducing end (RE).
27923477	10	40	theme	undesired	1815:1823	arg1	modifications					1836:1848	undesired structural modifications	1815:1848	undesired structural modifications resulting from side reactions	1815:1878	Each group of building blocks revealed different aspects of the properties of LMWHs, such as functional motifs required for anticoagulant activity, the structure of heparin starting materials, cleavage sites in the depolymerization reaction, and undesired structural modifications resulting from side reactions.
27923477	6	41	theme	nitrous	1026:1032	arg1	depolymerization					1039:1054	nitrous acid depolymerization	1026:1054	nitrous acid depolymerization	1026:1054	A sensitive and comprehensive approach is needed for detailed investigation of the structure of LMWHs prepared through nitrous acid depolymerization, especially their characteristic saturated non-reducing end (NRE) and 2,5-anhydro-d-mannitol reducing end (RE).
27923477	7	42	dep	modified	1178:1185	arg1	maltose					1170:1176	maltose	1170:1176	maltose	1170:1176	A maltose modified hydrophilic interaction column offers improved separation of complicated mixtures of acidic disaccharides and oligosaccharides.
27923477	7	43	theme	complicated	1248:1258	arg1	mixtures					1260:1267	complicated mixtures	1248:1267	complicated mixtures of acidic disaccharides and oligosaccharides	1248:1312	A maltose modified hydrophilic interaction column offers improved separation of complicated mixtures of acidic disaccharides and oligosaccharides.
27923477	9	44	theme	samples	1560:1566	arg1	analysis					1522:1529	the analysis	1518:1529	the analysis of dalteparin and nadroparin samples	1518:1566	Multiple reaction monitoring (MRM) MS/MS quantification was developed and validated in the analysis of dalteparin and nadroparin samples.
27923477	10	45	from	sites	1771:1775	arg1	reaction					1801:1808	the depolymerization reaction	1780:1808	the depolymerization reaction	1780:1808	Each group of building blocks revealed different aspects of the properties of LMWHs, such as functional motifs required for anticoagulant activity, the structure of heparin starting materials, cleavage sites in the depolymerization reaction, and undesired structural modifications resulting from side reactions.
27923477	1	46	theme	Low	208:210	arg1	drugs					274:278	important anticoagulant drugs	250:278	important anticoagulant drugs that are prepared through depolymerization of unfractionated heparin	250:347	Low molecular weight heparins (LMWHs) are important anticoagulant drugs that are prepared through depolymerization of unfractionated heparin.
27923477	1	46	theme	Low	208:210	arg1	LMWHs					239:243	LMWHs	239:243	LMWHs	239:243	Low molecular weight heparins (LMWHs) are important anticoagulant drugs that are prepared through depolymerization of unfractionated heparin.
27923477	1	46	theme	Low	208:210	arg1	heparins					229:236	Low molecular weight heparins	208:236	Low molecular weight heparins (LMWHs)	208:244	Low molecular weight heparins (LMWHs) are important anticoagulant drugs that are prepared through depolymerization of unfractionated heparin.
27923477	7	47	theme	acidic	1272:1277	arg1	disaccharides					1279:1291	acidic disaccharides	1272:1291	acidic disaccharides	1272:1291	A maltose modified hydrophilic interaction column offers improved separation of complicated mixtures of acidic disaccharides and oligosaccharides.
27923477	4	48	theme	effective	739:747	arg1	way					749:751	an effective way	736:751	an effective way to provide structural information on heparin and LMWHs	736:806	Compositional analysis of their basic building blocks is an effective way to provide structural information on heparin and LMWHs.
27923477	4	48	theme	effective	739:747	arg1	analysis					693:700	Compositional analysis	679:700	Compositional analysis of their basic building blocks	679:731	Compositional analysis of their basic building blocks is an effective way to provide structural information on heparin and LMWHs.
27923477	7	49	theme	interaction	1199:1209	arg1	column					1211:1216	A maltose modified hydrophilic interaction column	1168:1216	A maltose modified hydrophilic interaction column	1168:1216	A maltose modified hydrophilic interaction column offers improved separation of complicated mixtures of acidic disaccharides and oligosaccharides.
27923477	1	50	theme	weight	222:227	arg1	drugs					274:278	important anticoagulant drugs	250:278	important anticoagulant drugs that are prepared through depolymerization of unfractionated heparin	250:347	Low molecular weight heparins (LMWHs) are important anticoagulant drugs that are prepared through depolymerization of unfractionated heparin.
27923477	1	50	theme	weight	222:227	arg1	LMWHs					239:243	LMWHs	239:243	LMWHs	239:243	Low molecular weight heparins (LMWHs) are important anticoagulant drugs that are prepared through depolymerization of unfractionated heparin.
27923477	1	50	theme	weight	222:227	arg1	heparins					229:236	Low molecular weight heparins	208:236	Low molecular weight heparins (LMWHs)	208:244	Low molecular weight heparins (LMWHs) are important anticoagulant drugs that are prepared through depolymerization of unfractionated heparin.
27923477	6	51	theme	non-reducing	1099:1110	arg1	NRE					1117:1119	NRE	1117:1119	NRE	1117:1119	A sensitive and comprehensive approach is needed for detailed investigation of the structure of LMWHs prepared through nitrous acid depolymerization, especially their characteristic saturated non-reducing end (NRE) and 2,5-anhydro-d-mannitol reducing end (RE).
27923477	6	51	theme	non-reducing	1099:1110	arg1	end					1112:1114	their characteristic saturated non-reducing end	1068:1114	their characteristic saturated non-reducing end (NRE)	1068:1120	A sensitive and comprehensive approach is needed for detailed investigation of the structure of LMWHs prepared through nitrous acid depolymerization, especially their characteristic saturated non-reducing end (NRE) and 2,5-anhydro-d-mannitol reducing end (RE).
27923477	6	51	theme	non-reducing	1099:1110	arg1	LMWHs					1003:1007	LMWHs	1003:1007	LMWHs prepared through nitrous acid depolymerization	1003:1054	A sensitive and comprehensive approach is needed for detailed investigation of the structure of LMWHs prepared through nitrous acid depolymerization, especially their characteristic saturated non-reducing end (NRE) and 2,5-anhydro-d-mannitol reducing end (RE).
27923477	8	52	theme	tandem	1400:1405	arg1	MS					1426:1427	MS	1426:1427	MS	1426:1427	A total of 36 basic building blocks were unambiguously identified by high-resolution tandem mass spectrometry (MS).
27923477	8	52	theme	tandem	1400:1405	arg1	spectrometry					1412:1423	high-resolution tandem mass spectrometry	1384:1423	high-resolution tandem mass spectrometry (MS)	1384:1428	A total of 36 basic building blocks were unambiguously identified by high-resolution tandem mass spectrometry (MS).
27923477	10	53	theme	starting	1742:1749	arg1	materials					1751:1759	heparin starting materials	1734:1759	heparin starting materials	1734:1759	Each group of building blocks revealed different aspects of the properties of LMWHs, such as functional motifs required for anticoagulant activity, the structure of heparin starting materials, cleavage sites in the depolymerization reaction, and undesired structural modifications resulting from side reactions.
27923477	10	53	theme	starting	1742:1749	arg1	sites					1771:1775	cleavage sites	1762:1775	cleavage sites in the depolymerization reaction	1762:1808	Each group of building blocks revealed different aspects of the properties of LMWHs, such as functional motifs required for anticoagulant activity, the structure of heparin starting materials, cleavage sites in the depolymerization reaction, and undesired structural modifications resulting from side reactions.
27923477	7	54	theme	modified	1178:1185	arg1	column					1211:1216	A maltose modified hydrophilic interaction column	1168:1216	A maltose modified hydrophilic interaction column	1168:1216	A maltose modified hydrophilic interaction column offers improved separation of complicated mixtures of acidic disaccharides and oligosaccharides.
27923477	3	55	theme	acid	622:625	arg1	depolymerization					627:642	nitrous acid depolymerization	614:642	nitrous acid depolymerization followed by borohydride reduction	614:676	Enoxaparin is prepared by benzyl esterification and alkaline depolymerization, while dalteparin and nadroparin are prepared through nitrous acid depolymerization followed by borohydride reduction.
27923477	8	56	theme	basic	1329:1333	arg1	blocks					1344:1349	36 basic building blocks	1326:1349	36 basic building blocks	1326:1349	A total of 36 basic building blocks were unambiguously identified by high-resolution tandem mass spectrometry (MS).
27923477	6	57	theme	characteristic	1074:1087	arg1	NRE					1117:1119	NRE	1117:1119	NRE	1117:1119	A sensitive and comprehensive approach is needed for detailed investigation of the structure of LMWHs prepared through nitrous acid depolymerization, especially their characteristic saturated non-reducing end (NRE) and 2,5-anhydro-d-mannitol reducing end (RE).
27923477	6	57	theme	characteristic	1074:1087	arg1	end					1112:1114	their characteristic saturated non-reducing end	1068:1114	their characteristic saturated non-reducing end (NRE)	1068:1120	A sensitive and comprehensive approach is needed for detailed investigation of the structure of LMWHs prepared through nitrous acid depolymerization, especially their characteristic saturated non-reducing end (NRE) and 2,5-anhydro-d-mannitol reducing end (RE).
27923477	6	57	theme	characteristic	1074:1087	arg1	LMWHs					1003:1007	LMWHs	1003:1007	LMWHs prepared through nitrous acid depolymerization	1003:1054	A sensitive and comprehensive approach is needed for detailed investigation of the structure of LMWHs prepared through nitrous acid depolymerization, especially their characteristic saturated non-reducing end (NRE) and 2,5-anhydro-d-mannitol reducing end (RE).
27923477	4	58	theme	building	717:724	arg1	blocks					726:731	their basic building blocks	705:731	their basic building blocks	705:731	Compositional analysis of their basic building blocks is an effective way to provide structural information on heparin and LMWHs.
27923477	8	59	theme	blocks	1344:1349	arg1	total					1317:1321	A total	1315:1321	A total of 36 basic building blocks	1315:1349	A total of 36 basic building blocks were unambiguously identified by high-resolution tandem mass spectrometry (MS).
27923477	0	60	theme	acid	185:188	arg1	depolymerization					190:205	nitrous acid depolymerization	177:205	nitrous acid depolymerization	177:205	Hydrophilic interaction chromatography-multiple reaction monitoring mass spectrometry method for basic building block analysis of low molecular weight heparins prepared through nitrous acid depolymerization.
27923477	3	61	theme	borohydride	656:666	arg1	reduction					668:676	borohydride reduction	656:676	borohydride reduction	656:676	Enoxaparin is prepared by benzyl esterification and alkaline depolymerization, while dalteparin and nadroparin are prepared through nitrous acid depolymerization followed by borohydride reduction.
27923477	0	62	theme	heparins	151:158	arg1	analysis					118:125	analysis	118:125	analysis of low molecular weight heparins prepared through nitrous acid depolymerization	118:205	Hydrophilic interaction chromatography-multiple reaction monitoring mass spectrometry method for basic building block analysis of low molecular weight heparins prepared through nitrous acid depolymerization.
27923477	10	63	theme	side	1865:1868	arg1	reactions					1870:1878	side reactions	1865:1878	side reactions	1865:1878	Each group of building blocks revealed different aspects of the properties of LMWHs, such as functional motifs required for anticoagulant activity, the structure of heparin starting materials, cleavage sites in the depolymerization reaction, and undesired structural modifications resulting from side reactions.
27923477	5	64	theme	analysis	845:852	arg1	methods					854:860	most current compositional analysis methods	818:860	most current compositional analysis methods	818:860	However, most current compositional analysis methods have been limited to heparin and enoxaparin.
27923477	6	65	theme	2,5-anhydro-d-mannitol	1126:1147	arg1	RE					1163:1164	RE	1163:1164	RE	1163:1164	A sensitive and comprehensive approach is needed for detailed investigation of the structure of LMWHs prepared through nitrous acid depolymerization, especially their characteristic saturated non-reducing end (NRE) and 2,5-anhydro-d-mannitol reducing end (RE).
27923477	6	65	theme	2,5-anhydro-d-mannitol	1126:1147	arg1	LMWHs					1003:1007	LMWHs	1003:1007	LMWHs prepared through nitrous acid depolymerization	1003:1054	A sensitive and comprehensive approach is needed for detailed investigation of the structure of LMWHs prepared through nitrous acid depolymerization, especially their characteristic saturated non-reducing end (NRE) and 2,5-anhydro-d-mannitol reducing end (RE).
27923477	6	65	theme	2,5-anhydro-d-mannitol	1126:1147	arg1	end					1158:1160	2,5-anhydro-d-mannitol reducing end	1126:1160	2,5-anhydro-d-mannitol reducing end (RE)	1126:1165	A sensitive and comprehensive approach is needed for detailed investigation of the structure of LMWHs prepared through nitrous acid depolymerization, especially their characteristic saturated non-reducing end (NRE) and 2,5-anhydro-d-mannitol reducing end (RE).
27923477	4	66	theme	structural	764:773	arg1	information					775:785	structural information	764:785	structural information on heparin and LMWHs	764:806	Compositional analysis of their basic building blocks is an effective way to provide structural information on heparin and LMWHs.
27923477	9	67	theme	Multiple	1431:1438	arg1	MRM					1461:1463	MRM	1461:1463	MRM	1461:1463	Multiple reaction monitoring (MRM) MS/MS quantification was developed and validated in the analysis of dalteparin and nadroparin samples.
27923477	9	67	theme	Multiple	1431:1438	arg1	monitoring					1449:1458	Multiple reaction monitoring	1431:1458	Multiple reaction monitoring (MRM) MS/MS quantification	1431:1485	Multiple reaction monitoring (MRM) MS/MS quantification was developed and validated in the analysis of dalteparin and nadroparin samples.
27923477	0	68	theme	weight	144:149	arg1	heparins					151:158	low molecular weight heparins	130:158	low molecular weight heparins prepared through nitrous acid depolymerization	130:205	Hydrophilic interaction chromatography-multiple reaction monitoring mass spectrometry method for basic building block analysis of low molecular weight heparins prepared through nitrous acid depolymerization.
27923477	10	69	theme	blocks	1592:1597	arg1	blocks					1592:1597	building blocks	1583:1597	building blocks	1583:1597	Each group of building blocks revealed different aspects of the properties of LMWHs, such as functional motifs required for anticoagulant activity, the structure of heparin starting materials, cleavage sites in the depolymerization reaction, and undesired structural modifications resulting from side reactions.
27923477	10	69	theme	blocks	1592:1597	arg1	group					1574:1578	Each group	1569:1578	Each group of building blocks	1569:1597	Each group of building blocks revealed different aspects of the properties of LMWHs, such as functional motifs required for anticoagulant activity, the structure of heparin starting materials, cleavage sites in the depolymerization reaction, and undesired structural modifications resulting from side reactions.
27923477	9	70	theme	monitoring	1449:1458	arg1	quantification					1472:1485	Multiple reaction monitoring (MRM) MS/MS quantification	1431:1485	Multiple reaction monitoring (MRM) MS/MS quantification	1431:1485	Multiple reaction monitoring (MRM) MS/MS quantification was developed and validated in the analysis of dalteparin and nadroparin samples.
27923477	0	71	theme	molecular	134:142	arg1	heparins					151:158	low molecular weight heparins	130:158	low molecular weight heparins prepared through nitrous acid depolymerization	130:205	Hydrophilic interaction chromatography-multiple reaction monitoring mass spectrometry method for basic building block analysis of low molecular weight heparins prepared through nitrous acid depolymerization.
27923477	1	72	theme	anticoagulant	260:272	arg1	drugs					274:278	important anticoagulant drugs	250:278	important anticoagulant drugs that are prepared through depolymerization of unfractionated heparin	250:347	Low molecular weight heparins (LMWHs) are important anticoagulant drugs that are prepared through depolymerization of unfractionated heparin.
27923477	1	72	theme	anticoagulant	260:272	arg1	heparins					229:236	Low molecular weight heparins	208:236	Low molecular weight heparins (LMWHs)	208:244	Low molecular weight heparins (LMWHs) are important anticoagulant drugs that are prepared through depolymerization of unfractionated heparin.
27923477	5	73	theme	current	823:829	arg1	methods					854:860	most current compositional analysis methods	818:860	most current compositional analysis methods	818:860	However, most current compositional analysis methods have been limited to heparin and enoxaparin.
27923477	0	74	theme	interaction	12:22	arg1	spectrometry					73:84	Hydrophilic interaction chromatography-multiple reaction monitoring mass spectrometry	0:84	Hydrophilic interaction chromatography-multiple reaction monitoring mass spectrometry method for basic building	0:110	Hydrophilic interaction chromatography-multiple reaction monitoring mass spectrometry method for basic building block analysis of low molecular weight heparins prepared through nitrous acid depolymerization.
27923477	9	75	theme	MS/MS	1466:1470	arg1	quantification					1472:1485	Multiple reaction monitoring (MRM) MS/MS quantification	1431:1485	Multiple reaction monitoring (MRM) MS/MS quantification	1431:1485	Multiple reaction monitoring (MRM) MS/MS quantification was developed and validated in the analysis of dalteparin and nadroparin samples.
27923477	10	76	theme	different	1608:1616	arg1	aspects					1618:1624	different aspects	1608:1624	different aspects of the properties of LMWHs, such as functional motifs required for anticoagulant activity, the structure of heparin starting materials, cleavage sites in the depolymerization reaction, and undesired structural modifications resulting from side reactions	1608:1878	Each group of building blocks revealed different aspects of the properties of LMWHs, such as functional motifs required for anticoagulant activity, the structure of heparin starting materials, cleavage sites in the depolymerization reaction, and undesired structural modifications resulting from side reactions.
27923477	2	77	theme	products	419:426	arg1	structures					401:410	the structures	397:410	the structures of the products	397:426	Based on the types of processing reactions and the structures of the products, LMWHs can be divided into different classifications.
27923477	2	77	theme	products	419:426	arg1	types					363:367	the types	359:367	the types of processing reactions	359:391	Based on the types of processing reactions and the structures of the products, LMWHs can be divided into different classifications.
27923477	7	78	theme	oligosaccharides	1297:1312	arg1	mixtures					1260:1267	complicated mixtures	1248:1267	complicated mixtures of acidic disaccharides and oligosaccharides	1248:1312	A maltose modified hydrophilic interaction column offers improved separation of complicated mixtures of acidic disaccharides and oligosaccharides.
27923477	1	79	theme	unfractionated	326:339	arg1	heparin					341:347	unfractionated heparin	326:347	unfractionated heparin	326:347	Low molecular weight heparins (LMWHs) are important anticoagulant drugs that are prepared through depolymerization of unfractionated heparin.
27923477	10	80	theme	LMWHs	1647:1651	arg1	properties					1633:1642	the properties	1629:1642	the properties of LMWHs, such as functional motifs required for anticoagulant activity, the structure of heparin starting materials, cleavage sites in the depolymerization reaction, and undesired structural modifications resulting from side reactions	1629:1878	Each group of building blocks revealed different aspects of the properties of LMWHs, such as functional motifs required for anticoagulant activity, the structure of heparin starting materials, cleavage sites in the depolymerization reaction, and undesired structural modifications resulting from side reactions.
27923477	7	81	theme	disaccharides	1279:1291	arg1	mixtures					1260:1267	complicated mixtures	1248:1267	complicated mixtures of acidic disaccharides and oligosaccharides	1248:1312	A maltose modified hydrophilic interaction column offers improved separation of complicated mixtures of acidic disaccharides and oligosaccharides.
27923477	6	82	theme	detailed	960:967	arg1	investigation					969:981	detailed investigation	960:981	detailed investigation of the structure of LMWHs prepared through nitrous acid depolymerization, especially their characteristic saturated non-reducing end (NRE) and 2,5-anhydro-d-mannitol reducing end (RE)	960:1165	A sensitive and comprehensive approach is needed for detailed investigation of the structure of LMWHs prepared through nitrous acid depolymerization, especially their characteristic saturated non-reducing end (NRE) and 2,5-anhydro-d-mannitol reducing end (RE).
27923477	10	83	theme	properties	1633:1642	arg1	aspects					1618:1624	different aspects	1608:1624	different aspects of the properties of LMWHs, such as functional motifs required for anticoagulant activity, the structure of heparin starting materials, cleavage sites in the depolymerization reaction, and undesired structural modifications resulting from side reactions	1608:1878	Each group of building blocks revealed different aspects of the properties of LMWHs, such as functional motifs required for anticoagulant activity, the structure of heparin starting materials, cleavage sites in the depolymerization reaction, and undesired structural modifications resulting from side reactions.
27923477	5	84	theme	compositional	831:843	arg1	methods					854:860	most current compositional analysis methods	818:860	most current compositional analysis methods	818:860	However, most current compositional analysis methods have been limited to heparin and enoxaparin.
27923477	10	85	theme	functional	1662:1671	arg1	motifs					1673:1678	functional motifs	1662:1678	functional motifs required for anticoagulant activity, the structure of heparin starting materials, cleavage sites in the depolymerization reaction, and undesired structural modifications resulting from side reactions	1662:1878	Each group of building blocks revealed different aspects of the properties of LMWHs, such as functional motifs required for anticoagulant activity, the structure of heparin starting materials, cleavage sites in the depolymerization reaction, and undesired structural modifications resulting from side reactions.
27923477	2	86	theme	reactions	383:391	arg1	structures					401:410	the structures	397:410	the structures of the products	397:426	Based on the types of processing reactions and the structures of the products, LMWHs can be divided into different classifications.
27923477	2	86	theme	reactions	383:391	arg1	types					363:367	the types	359:367	the types of processing reactions	359:391	Based on the types of processing reactions and the structures of the products, LMWHs can be divided into different classifications.
27923477	3	87	theme	alkaline	534:541	arg1	depolymerization					543:558	alkaline depolymerization	534:558	alkaline depolymerization	534:558	Enoxaparin is prepared by benzyl esterification and alkaline depolymerization, while dalteparin and nadroparin are prepared through nitrous acid depolymerization followed by borohydride reduction.
27923477	9	88	theme	dalteparin	1534:1543	arg1	samples					1560:1566	dalteparin and nadroparin samples	1534:1566	dalteparin and nadroparin samples	1534:1566	Multiple reaction monitoring (MRM) MS/MS quantification was developed and validated in the analysis of dalteparin and nadroparin samples.
27923477	4	89	theme	basic	711:715	arg1	blocks					726:731	their basic building blocks	705:731	their basic building blocks	705:731	Compositional analysis of their basic building blocks is an effective way to provide structural information on heparin and LMWHs.
27923477	10	90	theme	anticoagulant	1693:1705	arg1	activity					1707:1714	anticoagulant activity	1693:1714	anticoagulant activity	1693:1714	Each group of building blocks revealed different aspects of the properties of LMWHs, such as functional motifs required for anticoagulant activity, the structure of heparin starting materials, cleavage sites in the depolymerization reaction, and undesired structural modifications resulting from side reactions.
27923477	9	91	theme	nadroparin	1549:1558	arg1	samples					1560:1566	dalteparin and nadroparin samples	1534:1566	dalteparin and nadroparin samples	1534:1566	Multiple reaction monitoring (MRM) MS/MS quantification was developed and validated in the analysis of dalteparin and nadroparin samples.
28262124	4	0	theme	composition	683:693	arg1	analysis					695:702	composition analysis	683:702	composition analysis	683:702	Two well-established methods, determination of the total polysaccharides content and monosaccharides composition analysis, were both employed.
28262124	7	1	with	acid	1261:1264	arg1	concentrations					1295:1308	the opposite concentrations	1282:1308	the opposite concentrations in the two drugs	1282:1325	Principal component analysis and orthogonal partial least squares discriminant analysis using the peak areas of monosaccharides derivatives as the variables were performed, and the results indicated that mannuronic acid and xylose with the opposite concentrations in the two drugs were the differential components.
28262124	1	2	theme	Chinese	169:175	arg1	medicines					177:185	numerous traditional Chinese medicines	148:185	numerous traditional Chinese medicines	148:185	Polysaccharides from numerous traditional Chinese medicines have been proven as the bioactive ingredients and are hence used as the quality control markers.
28262124	5	3	theme	evaluation	766:775	arg1	criteria					777:784	the evaluation criteria	762:784	the evaluation criteria of the polysaccharides contents of not less than 2.0% and 1.7%	762:847	Based upon the quantitative results, the evaluation criteria of the polysaccharides contents of not less than 2.0% and 1.7% were proposed for sea-tangle and sargassum, respectively.
28262124	7	4	theme	peak	1144:1147	arg1	areas					1149:1153	the peak areas	1140:1153	the peak areas of monosaccharides derivatives	1140:1184	Principal component analysis and orthogonal partial least squares discriminant analysis using the peak areas of monosaccharides derivatives as the variables were performed, and the results indicated that mannuronic acid and xylose with the opposite concentrations in the two drugs were the differential components.
28262124	7	4	theme	peak	1144:1147	arg1	variables					1193:1201	the variables	1189:1201	the variables	1189:1201	Principal component analysis and orthogonal partial least squares discriminant analysis using the peak areas of monosaccharides derivatives as the variables were performed, and the results indicated that mannuronic acid and xylose with the opposite concentrations in the two drugs were the differential components.
28262124	2	5	theme	due	349:351	arg1	specificity					336:346	a poor specificity	329:346	a poor specificity	329:346	However, the assessment criteria always show a poor specificity, due to the lack of systematic comparison among the analogous herbs.
28262124	1	6	theme	quality	259:265	arg1	Polysaccharides					127:141	Polysaccharides	127:141	Polysaccharides from numerous traditional Chinese medicines	127:185	Polysaccharides from numerous traditional Chinese medicines have been proven as the bioactive ingredients and are hence used as the quality control markers.
28262124	1	6	theme	quality	259:265	arg1	markers					275:281	the quality control markers	255:281	the quality control markers	255:281	Polysaccharides from numerous traditional Chinese medicines have been proven as the bioactive ingredients and are hence used as the quality control markers.
28262124	8	7	theme	area	1403:1406	arg1	ratio					1408:1412	the peak area ratio	1394:1412	the peak area ratio of these two monosaccharides derivatives	1394:1453	A discriminative criterion using the peak area ratio of these two monosaccharides derivatives was proposed for the qualitative identification.
28262124	6	8	dep	hydrolysis	991:1000	arg1	the					987:989	the	987:989	the	987:989	Nine identical monosaccharide derivatives appeared on the HPLC chromatograms of the hydrolysis and derivatized solutions of the two drugs.
28262124	7	9	with	xylose	1270:1275	arg1	concentrations					1295:1308	the opposite concentrations	1282:1308	the opposite concentrations in the two drugs	1282:1325	Principal component analysis and orthogonal partial least squares discriminant analysis using the peak areas of monosaccharides derivatives as the variables were performed, and the results indicated that mannuronic acid and xylose with the opposite concentrations in the two drugs were the differential components.
28262124	1	10	from	medicines	177:185	arg1	Polysaccharides					127:141	Polysaccharides	127:141	Polysaccharides from numerous traditional Chinese medicines	127:185	Polysaccharides from numerous traditional Chinese medicines have been proven as the bioactive ingredients and are hence used as the quality control markers.
28262124	1	10	from	medicines	177:185	arg1	ingredients					221:231	the bioactive ingredients	207:231	the bioactive ingredients	207:231	Polysaccharides from numerous traditional Chinese medicines have been proven as the bioactive ingredients and are hence used as the quality control markers.
28262124	1	10	from	medicines	177:185	arg1	markers					275:281	the quality control markers	255:281	the quality control markers	255:281	Polysaccharides from numerous traditional Chinese medicines have been proven as the bioactive ingredients and are hence used as the quality control markers.
28262124	8	11	theme	discriminative	1363:1376	arg1	criterion					1378:1386	A discriminative criterion	1361:1386	A discriminative criterion using the peak area ratio of these two monosaccharides derivatives	1361:1453	A discriminative criterion using the peak area ratio of these two monosaccharides derivatives was proposed for the qualitative identification.
28262124	4	12	theme	polysaccharides	639:653	arg1	content					655:661	the total polysaccharides content	629:661	the total polysaccharides content	629:661	Two well-established methods, determination of the total polysaccharides content and monosaccharides composition analysis, were both employed.
28262124	1	13	theme	control	267:273	arg1	Polysaccharides					127:141	Polysaccharides	127:141	Polysaccharides from numerous traditional Chinese medicines	127:185	Polysaccharides from numerous traditional Chinese medicines have been proven as the bioactive ingredients and are hence used as the quality control markers.
28262124	1	13	theme	control	267:273	arg1	markers					275:281	the quality control markers	255:281	the quality control markers	255:281	Polysaccharides from numerous traditional Chinese medicines have been proven as the bioactive ingredients and are hence used as the quality control markers.
28262124	0	14	theme	polysaccharides	80:94	arg1	control					65:71	the quality control	53:71	the quality control of the polysaccharides from sea-tangle and sargassum	53:124	Development of specific and quantitative methods for the quality control of the polysaccharides from sea-tangle and sargassum.
28262124	9	15	theme	quantitative	1539:1550	arg1	method					1568:1573	a more specific and quantitative quality control method	1519:1573	a more specific and quantitative quality control method	1519:1573	In conclusion, a more specific and quantitative quality control method was developed for sea-tangle and sargassum.
28262124	6	16	theme	derivatized	1006:1016	arg1	solutions					1018:1026	derivatized solutions	1006:1026	derivatized solutions	1006:1026	Nine identical monosaccharide derivatives appeared on the HPLC chromatograms of the hydrolysis and derivatized solutions of the two drugs.
28262124	7	17	theme	squares	1104:1110	arg1	analysis					1125:1132	orthogonal partial least squares discriminant analysis	1079:1132	orthogonal partial least squares discriminant analysis	1079:1132	Principal component analysis and orthogonal partial least squares discriminant analysis using the peak areas of monosaccharides derivatives as the variables were performed, and the results indicated that mannuronic acid and xylose with the opposite concentrations in the two drugs were the differential components.
28262124	5	18	theme	2.0	835:837	arg1	%					838:838	%	838:838	%	838:838	Based upon the quantitative results, the evaluation criteria of the polysaccharides contents of not less than 2.0% and 1.7% were proposed for sea-tangle and sargassum, respectively.
28262124	3	19	theme	present	424:430	arg1	study					432:436	the present study	420:436	the present study	420:436	In the present study, two similar materials, namely sea-tangle and sargassum, were selected as the model herbs to develop more specific methods for quality control.
28262124	5	20	theme	quantitative	740:751	arg1	results					753:759	the quantitative results	736:759	the quantitative results	736:759	Based upon the quantitative results, the evaluation criteria of the polysaccharides contents of not less than 2.0% and 1.7% were proposed for sea-tangle and sargassum, respectively.
28262124	7	21	theme	monosaccharides	1158:1172	arg1	derivatives					1174:1184	monosaccharides derivatives	1158:1184	monosaccharides derivatives	1158:1184	Principal component analysis and orthogonal partial least squares discriminant analysis using the peak areas of monosaccharides derivatives as the variables were performed, and the results indicated that mannuronic acid and xylose with the opposite concentrations in the two drugs were the differential components.
28262124	2	22	theme	comparison	379:388	arg1	lack					360:363	the lack	356:363	the lack of systematic comparison among the analogous herbs	356:414	However, the assessment criteria always show a poor specificity, due to the lack of systematic comparison among the analogous herbs.
28262124	5	23	theme	contents	809:816	arg1	criteria					777:784	the evaluation criteria	762:784	the evaluation criteria of the polysaccharides contents of not less than 2.0% and 1.7%	762:847	Based upon the quantitative results, the evaluation criteria of the polysaccharides contents of not less than 2.0% and 1.7% were proposed for sea-tangle and sargassum, respectively.
28262124	7	24	theme	partial	1090:1096	arg1	squares					1104:1110	partial least squares	1090:1110	orthogonal partial least squares discriminant analysis	1079:1132	Principal component analysis and orthogonal partial least squares discriminant analysis using the peak areas of monosaccharides derivatives as the variables were performed, and the results indicated that mannuronic acid and xylose with the opposite concentrations in the two drugs were the differential components.
28262124	8	25	theme	peak	1398:1401	arg1	ratio					1408:1412	the peak area ratio	1394:1412	the peak area ratio of these two monosaccharides derivatives	1394:1453	A discriminative criterion using the peak area ratio of these two monosaccharides derivatives was proposed for the qualitative identification.
28262124	2	26	theme	systematic	368:377	arg1	comparison					379:388	systematic comparison	368:388	systematic comparison among the analogous herbs	368:414	However, the assessment criteria always show a poor specificity, due to the lack of systematic comparison among the analogous herbs.
28262124	7	27	theme	derivatives	1174:1184	arg1	areas					1149:1153	the peak areas	1140:1153	the peak areas of monosaccharides derivatives	1140:1184	Principal component analysis and orthogonal partial least squares discriminant analysis using the peak areas of monosaccharides derivatives as the variables were performed, and the results indicated that mannuronic acid and xylose with the opposite concentrations in the two drugs were the differential components.
28262124	7	27	theme	derivatives	1174:1184	arg1	variables					1193:1201	the variables	1189:1201	the variables	1189:1201	Principal component analysis and orthogonal partial least squares discriminant analysis using the peak areas of monosaccharides derivatives as the variables were performed, and the results indicated that mannuronic acid and xylose with the opposite concentrations in the two drugs were the differential components.
28262124	7	28	theme	least	1098:1102	arg1	squares					1104:1110	partial least squares	1090:1110	orthogonal partial least squares discriminant analysis	1079:1132	Principal component analysis and orthogonal partial least squares discriminant analysis using the peak areas of monosaccharides derivatives as the variables were performed, and the results indicated that mannuronic acid and xylose with the opposite concentrations in the two drugs were the differential components.
28262124	7	29	from	concentrations	1295:1308	arg1	drugs					1321:1325	the two drugs	1313:1325	the two drugs	1313:1325	Principal component analysis and orthogonal partial least squares discriminant analysis using the peak areas of monosaccharides derivatives as the variables were performed, and the results indicated that mannuronic acid and xylose with the opposite concentrations in the two drugs were the differential components.
28262124	3	30	theme	specific	544:551	arg1	methods					553:559	more specific methods	539:559	more specific methods	539:559	In the present study, two similar materials, namely sea-tangle and sargassum, were selected as the model herbs to develop more specific methods for quality control.
28262124	4	31	theme	content	655:661	arg1	monosaccharides					667:681	monosaccharides	667:681	monosaccharides	667:681	Two well-established methods, determination of the total polysaccharides content and monosaccharides composition analysis, were both employed.
28262124	4	31	theme	content	655:661	arg1	determination					612:624	determination	612:624	determination of the total polysaccharides content	612:661	Two well-established methods, determination of the total polysaccharides content and monosaccharides composition analysis, were both employed.
28262124	4	31	theme	content	655:661	arg1	methods					603:609	Two well-established methods	582:609	Two well-established methods	582:609	Two well-established methods, determination of the total polysaccharides content and monosaccharides composition analysis, were both employed.
28262124	6	32	theme	HPLC	965:968	arg1	chromatograms					970:982	the HPLC chromatograms	961:982	the HPLC chromatograms of the hydrolysis and derivatized solutions of the two drugs	961:1043	Nine identical monosaccharide derivatives appeared on the HPLC chromatograms of the hydrolysis and derivatized solutions of the two drugs.
28262124	7	33	theme	discriminant	1112:1123	arg1	analysis					1125:1132	orthogonal partial least squares discriminant analysis	1079:1132	orthogonal partial least squares discriminant analysis	1079:1132	Principal component analysis and orthogonal partial least squares discriminant analysis using the peak areas of monosaccharides derivatives as the variables were performed, and the results indicated that mannuronic acid and xylose with the opposite concentrations in the two drugs were the differential components.
28262124	3	34	theme	similar	443:449	arg1	model					516:520	the model herbs to develop more specific methods for quality control	512:579	the model herbs to develop more specific methods for quality control	512:579	In the present study, two similar materials, namely sea-tangle and sargassum, were selected as the model herbs to develop more specific methods for quality control.
28262124	3	34	theme	similar	443:449	arg1	sea-tangle					469:478	sea-tangle	469:478	sea-tangle	469:478	In the present study, two similar materials, namely sea-tangle and sargassum, were selected as the model herbs to develop more specific methods for quality control.
28262124	3	34	theme	similar	443:449	arg1	sargassum					484:492	sargassum	484:492	sargassum	484:492	In the present study, two similar materials, namely sea-tangle and sargassum, were selected as the model herbs to develop more specific methods for quality control.
28262124	3	34	theme	similar	443:449	arg1	materials					451:459	two similar materials	439:459	two similar materials	439:459	In the present study, two similar materials, namely sea-tangle and sargassum, were selected as the model herbs to develop more specific methods for quality control.
28262124	0	35	theme	specific	15:22	arg1	methods					41:47	specific and quantitative methods	15:47	specific and quantitative methods for the quality control of the polysaccharides from sea-tangle and sargassum	15:124	Development of specific and quantitative methods for the quality control of the polysaccharides from sea-tangle and sargassum.
28262124	7	36	theme	orthogonal	1079:1088	arg1	analysis					1125:1132	orthogonal partial least squares discriminant analysis	1079:1132	orthogonal partial least squares discriminant analysis	1079:1132	Principal component analysis and orthogonal partial least squares discriminant analysis using the peak areas of monosaccharides derivatives as the variables were performed, and the results indicated that mannuronic acid and xylose with the opposite concentrations in the two drugs were the differential components.
28262124	8	37	theme	qualitative	1476:1486	arg1	identification					1488:1501	the qualitative identification	1472:1501	the qualitative identification	1472:1501	A discriminative criterion using the peak area ratio of these two monosaccharides derivatives was proposed for the qualitative identification.
28262124	2	38	theme	assessment	297:306	arg1	criteria					308:315	the assessment criteria	293:315	the assessment criteria	293:315	However, the assessment criteria always show a poor specificity, due to the lack of systematic comparison among the analogous herbs.
28262124	1	39	theme	bioactive	211:219	arg1	Polysaccharides					127:141	Polysaccharides	127:141	Polysaccharides from numerous traditional Chinese medicines	127:185	Polysaccharides from numerous traditional Chinese medicines have been proven as the bioactive ingredients and are hence used as the quality control markers.
28262124	1	39	theme	bioactive	211:219	arg1	ingredients					221:231	the bioactive ingredients	207:231	the bioactive ingredients	207:231	Polysaccharides from numerous traditional Chinese medicines have been proven as the bioactive ingredients and are hence used as the quality control markers.
28262124	0	40	theme	methods	41:47	arg1	Development					0:10	Development	0:10	Development of specific and quantitative methods for the quality control of the polysaccharides from sea-tangle and sargassum.	0:125	Development of specific and quantitative methods for the quality control of the polysaccharides from sea-tangle and sargassum.
28262124	7	41	theme	component	1056:1064	arg1	analysis					1066:1073	Principal component analysis	1046:1073	Principal component analysis	1046:1073	Principal component analysis and orthogonal partial least squares discriminant analysis using the peak areas of monosaccharides derivatives as the variables were performed, and the results indicated that mannuronic acid and xylose with the opposite concentrations in the two drugs were the differential components.
28262124	6	42	theme	drugs	1039:1043	arg1	hydrolysis					991:1000	hydrolysis	991:1000	hydrolysis	991:1000	Nine identical monosaccharide derivatives appeared on the HPLC chromatograms of the hydrolysis and derivatized solutions of the two drugs.
28262124	6	42	theme	drugs	1039:1043	arg1	solutions					1018:1026	derivatized solutions	1006:1026	derivatized solutions	1006:1026	Nine identical monosaccharide derivatives appeared on the HPLC chromatograms of the hydrolysis and derivatized solutions of the two drugs.
28262124	3	43	theme	quality	565:571	arg1	control					573:579	quality control	565:579	quality control	565:579	In the present study, two similar materials, namely sea-tangle and sargassum, were selected as the model herbs to develop more specific methods for quality control.
28262124	7	44	theme	differential	1336:1347	arg1	xylose					1270:1275	xylose	1270:1275	xylose	1270:1275	Principal component analysis and orthogonal partial least squares discriminant analysis using the peak areas of monosaccharides derivatives as the variables were performed, and the results indicated that mannuronic acid and xylose with the opposite concentrations in the two drugs were the differential components.
28262124	7	44	theme	differential	1336:1347	arg1	acid					1261:1264	mannuronic acid	1250:1264	mannuronic acid	1250:1264	Principal component analysis and orthogonal partial least squares discriminant analysis using the peak areas of monosaccharides derivatives as the variables were performed, and the results indicated that mannuronic acid and xylose with the opposite concentrations in the two drugs were the differential components.
28262124	7	44	theme	differential	1336:1347	arg1	components					1349:1358	the differential components	1332:1358	the differential components	1332:1358	Principal component analysis and orthogonal partial least squares discriminant analysis using the peak areas of monosaccharides derivatives as the variables were performed, and the results indicated that mannuronic acid and xylose with the opposite concentrations in the two drugs were the differential components.
28262124	2	45	theme	analogous	400:408	arg1	herbs					410:414	the analogous herbs	396:414	the analogous herbs	396:414	However, the assessment criteria always show a poor specificity, due to the lack of systematic comparison among the analogous herbs.
28262124	0	46	theme	quantitative	28:39	arg1	methods					41:47	specific and quantitative methods	15:47	specific and quantitative methods for the quality control of the polysaccharides from sea-tangle and sargassum	15:124	Development of specific and quantitative methods for the quality control of the polysaccharides from sea-tangle and sargassum.
28262124	5	47	theme	%	847:847	arg1	contents					809:816	the polysaccharides contents	789:816	the polysaccharides contents of not less than 2.0% and 1.7%	789:847	Based upon the quantitative results, the evaluation criteria of the polysaccharides contents of not less than 2.0% and 1.7% were proposed for sea-tangle and sargassum, respectively.
28262124	6	48	theme	hydrolysis	991:1000	arg1	chromatograms					970:982	the HPLC chromatograms	961:982	the HPLC chromatograms of the hydrolysis and derivatized solutions of the two drugs	961:1043	Nine identical monosaccharide derivatives appeared on the HPLC chromatograms of the hydrolysis and derivatized solutions of the two drugs.
28262124	0	49	from	control	65:71	arg1	sargassum					116:124	sargassum	116:124	sargassum	116:124	Development of specific and quantitative methods for the quality control of the polysaccharides from sea-tangle and sargassum.
28262124	0	49	from	control	65:71	arg1	sea-tangle					101:110	sea-tangle	101:110	sea-tangle	101:110	Development of specific and quantitative methods for the quality control of the polysaccharides from sea-tangle and sargassum.
28262124	7	50	theme	mannuronic	1250:1259	arg1	xylose					1270:1275	xylose	1270:1275	xylose	1270:1275	Principal component analysis and orthogonal partial least squares discriminant analysis using the peak areas of monosaccharides derivatives as the variables were performed, and the results indicated that mannuronic acid and xylose with the opposite concentrations in the two drugs were the differential components.
28262124	7	50	theme	mannuronic	1250:1259	arg1	acid					1261:1264	mannuronic acid	1250:1264	mannuronic acid	1250:1264	Principal component analysis and orthogonal partial least squares discriminant analysis using the peak areas of monosaccharides derivatives as the variables were performed, and the results indicated that mannuronic acid and xylose with the opposite concentrations in the two drugs were the differential components.
28262124	7	50	theme	mannuronic	1250:1259	arg1	components					1349:1358	the differential components	1332:1358	the differential components	1332:1358	Principal component analysis and orthogonal partial least squares discriminant analysis using the peak areas of monosaccharides derivatives as the variables were performed, and the results indicated that mannuronic acid and xylose with the opposite concentrations in the two drugs were the differential components.
28262124	4	51	dep	determination	612:624	arg1	analysis					695:702	composition analysis	683:702	composition analysis	683:702	Two well-established methods, determination of the total polysaccharides content and monosaccharides composition analysis, were both employed.
28262124	5	52	theme	polysaccharides	793:807	arg1	contents					809:816	the polysaccharides contents	789:816	the polysaccharides contents of not less than 2.0% and 1.7%	789:847	Based upon the quantitative results, the evaluation criteria of the polysaccharides contents of not less than 2.0% and 1.7% were proposed for sea-tangle and sargassum, respectively.
28262124	6	53	theme	monosaccharide	922:935	arg1	derivatives					937:947	Nine identical monosaccharide derivatives	907:947	Nine identical monosaccharide derivatives	907:947	Nine identical monosaccharide derivatives appeared on the HPLC chromatograms of the hydrolysis and derivatized solutions of the two drugs.
28262124	7	54	theme	Principal	1046:1054	arg1	analysis					1066:1073	Principal component analysis	1046:1073	Principal component analysis	1046:1073	Principal component analysis and orthogonal partial least squares discriminant analysis using the peak areas of monosaccharides derivatives as the variables were performed, and the results indicated that mannuronic acid and xylose with the opposite concentrations in the two drugs were the differential components.
28262124	0	55	from	sea-tangle	101:110	arg1	control					65:71	the quality control	53:71	the quality control of the polysaccharides from sea-tangle and sargassum	53:124	Development of specific and quantitative methods for the quality control of the polysaccharides from sea-tangle and sargassum.
28262124	0	55	from	sea-tangle	101:110	arg1	polysaccharides					80:94	the polysaccharides	76:94	the polysaccharides from sea-tangle and sargassum	76:124	Development of specific and quantitative methods for the quality control of the polysaccharides from sea-tangle and sargassum.
28262124	9	56	theme	specific	1526:1533	arg1	method					1568:1573	a more specific and quantitative quality control method	1519:1573	a more specific and quantitative quality control method	1519:1573	In conclusion, a more specific and quantitative quality control method was developed for sea-tangle and sargassum.
28262124	6	57	theme	identical	912:920	arg1	derivatives					937:947	Nine identical monosaccharide derivatives	907:947	Nine identical monosaccharide derivatives	907:947	Nine identical monosaccharide derivatives appeared on the HPLC chromatograms of the hydrolysis and derivatized solutions of the two drugs.
28262124	1	58	used	used	247:250	arg2	markers					275:281	the quality control markers	255:281	the quality control markers	255:281	Polysaccharides from numerous traditional Chinese medicines have been proven as the bioactive ingredients and are hence used as the quality control markers.
28262124	1	58	used	used	247:250	arg2	ingredients					221:231	the bioactive ingredients	207:231	the bioactive ingredients	207:231	Polysaccharides from numerous traditional Chinese medicines have been proven as the bioactive ingredients and are hence used as the quality control markers.
28262124	1	58	used	used	247:250	arg2	Polysaccharides					127:141	Polysaccharides	127:141	Polysaccharides from numerous traditional Chinese medicines	127:185	Polysaccharides from numerous traditional Chinese medicines have been proven as the bioactive ingredients and are hence used as the quality control markers.
28262124	9	59	theme	control	1560:1566	arg1	method					1568:1573	a more specific and quantitative quality control method	1519:1573	a more specific and quantitative quality control method	1519:1573	In conclusion, a more specific and quantitative quality control method was developed for sea-tangle and sargassum.
28262124	2	60	theme	poor	331:334	arg1	specificity					336:346	a poor specificity	329:346	a poor specificity	329:346	However, the assessment criteria always show a poor specificity, due to the lack of systematic comparison among the analogous herbs.
28262124	8	61	theme	derivatives	1443:1453	arg1	ratio					1408:1412	the peak area ratio	1394:1412	the peak area ratio of these two monosaccharides derivatives	1394:1453	A discriminative criterion using the peak area ratio of these two monosaccharides derivatives was proposed for the qualitative identification.
28262124	7	62	theme	opposite	1286:1293	arg1	concentrations					1295:1308	the opposite concentrations	1282:1308	the opposite concentrations in the two drugs	1282:1325	Principal component analysis and orthogonal partial least squares discriminant analysis using the peak areas of monosaccharides derivatives as the variables were performed, and the results indicated that mannuronic acid and xylose with the opposite concentrations in the two drugs were the differential components.
28262124	8	63	theme	monosaccharides	1427:1441	arg1	derivatives					1443:1453	these two monosaccharides derivatives	1417:1453	these two monosaccharides derivatives	1417:1453	A discriminative criterion using the peak area ratio of these two monosaccharides derivatives was proposed for the qualitative identification.
28262124	9	64	theme	quality	1552:1558	arg1	method					1568:1573	a more specific and quantitative quality control method	1519:1573	a more specific and quantitative quality control method	1519:1573	In conclusion, a more specific and quantitative quality control method was developed for sea-tangle and sargassum.
28262124	1	65	theme	numerous	148:155	arg1	medicines					177:185	numerous traditional Chinese medicines	148:185	numerous traditional Chinese medicines	148:185	Polysaccharides from numerous traditional Chinese medicines have been proven as the bioactive ingredients and are hence used as the quality control markers.
28262124	4	66	theme	total	633:637	arg1	content					655:661	the total polysaccharides content	629:661	the total polysaccharides content	629:661	Two well-established methods, determination of the total polysaccharides content and monosaccharides composition analysis, were both employed.
28262124	6	67	theme	solutions	1018:1026	arg1	chromatograms					970:982	the HPLC chromatograms	961:982	the HPLC chromatograms of the hydrolysis and derivatized solutions of the two drugs	961:1043	Nine identical monosaccharide derivatives appeared on the HPLC chromatograms of the hydrolysis and derivatized solutions of the two drugs.
28262124	0	68	theme	quality	57:63	arg1	control					65:71	the quality control	53:71	the quality control of the polysaccharides from sea-tangle and sargassum	53:124	Development of specific and quantitative methods for the quality control of the polysaccharides from sea-tangle and sargassum.
28262124	4	69	theme	well-established	586:601	arg1	monosaccharides					667:681	monosaccharides	667:681	monosaccharides	667:681	Two well-established methods, determination of the total polysaccharides content and monosaccharides composition analysis, were both employed.
28262124	4	69	theme	well-established	586:601	arg1	determination					612:624	determination	612:624	determination of the total polysaccharides content	612:661	Two well-established methods, determination of the total polysaccharides content and monosaccharides composition analysis, were both employed.
28262124	4	69	theme	well-established	586:601	arg1	methods					603:609	Two well-established methods	582:609	Two well-established methods	582:609	Two well-established methods, determination of the total polysaccharides content and monosaccharides composition analysis, were both employed.
28262124	1	70	theme	traditional	157:167	arg1	medicines					177:185	numerous traditional Chinese medicines	148:185	numerous traditional Chinese medicines	148:185	Polysaccharides from numerous traditional Chinese medicines have been proven as the bioactive ingredients and are hence used as the quality control markers.
28262124	0	71	from	sargassum	116:124	arg1	control					65:71	the quality control	53:71	the quality control of the polysaccharides from sea-tangle and sargassum	53:124	Development of specific and quantitative methods for the quality control of the polysaccharides from sea-tangle and sargassum.
28262124	0	71	from	sargassum	116:124	arg1	polysaccharides					80:94	the polysaccharides	76:94	the polysaccharides from sea-tangle and sargassum	76:124	Development of specific and quantitative methods for the quality control of the polysaccharides from sea-tangle and sargassum.
26769222	0	0	theme	extracellular	71:83	arg1	substances					95:104	extracellular polymeric substances	71:104	extracellular polymeric substances from bacterial cultures	71:128	Proteins dominate in the surface layers formed on materials exposed to extracellular polymeric substances from bacterial cultures.
26769222	1	1	theme	surface	164:170	arg1	layers					185:190	the surface conditioning layers	160:190	the surface conditioning layers formed by different types of solutions (from isolated EPS to whole culture media), involving different bacterial strains relevant for biocorrosion	160:337	The chemical compositions of the surface conditioning layers formed by different types of solutions (from isolated EPS to whole culture media), involving different bacterial strains relevant for biocorrosion were compared, as they may influence the initial step in biofilm formation.
26769222	1	2	theme	whole	253:257	arg1	media					267:271	whole culture media	253:271	whole culture media	253:271	The chemical compositions of the surface conditioning layers formed by different types of solutions (from isolated EPS to whole culture media), involving different bacterial strains relevant for biocorrosion were compared, as they may influence the initial step in biofilm formation.
26769222	1	3	from	step	388:391	arg1	formation					404:412	biofilm formation	396:412	biofilm formation	396:412	The chemical compositions of the surface conditioning layers formed by different types of solutions (from isolated EPS to whole culture media), involving different bacterial strains relevant for biocorrosion were compared, as they may influence the initial step in biofilm formation.
26769222	4	4	theme	conditioning	793:804	arg1	layers					806:811	all conditioning layers	789:811	all conditioning layers	789:811	Proteins or peptides were found to be a major constituent of all conditioning layers and polysaccharides were not present in appreciable concentrations; the proportion of nitrogen which may be due to DNA was lower than 15%.
26769222	1	5	theme	culture	259:265	arg1	media					267:271	whole culture media	253:271	whole culture media	253:271	The chemical compositions of the surface conditioning layers formed by different types of solutions (from isolated EPS to whole culture media), involving different bacterial strains relevant for biocorrosion were compared, as they may influence the initial step in biofilm formation.
26769222	0	6	theme	polymeric	85:93	arg1	substances					95:104	extracellular polymeric substances	71:104	extracellular polymeric substances from bacterial cultures	71:128	Proteins dominate in the surface layers formed on materials exposed to extracellular polymeric substances from bacterial cultures.
26769222	4	7	theme	nitrogen	899:906	arg1	due					921:923	due	921:923	due	921:923	Proteins or peptides were found to be a major constituent of all conditioning layers and polysaccharides were not present in appreciable concentrations; the proportion of nitrogen which may be due to DNA was lower than 15%.
26769222	4	7	theme	nitrogen	899:906	arg1	lower					936:940	lower	936:940	lower	936:940	Proteins or peptides were found to be a major constituent of all conditioning layers and polysaccharides were not present in appreciable concentrations; the proportion of nitrogen which may be due to DNA was lower than 15%.
26769222	4	7	theme	nitrogen	899:906	arg1	proportion					885:894	the proportion	881:894	the proportion of nitrogen which may be due to DNA	881:930	Proteins or peptides were found to be a major constituent of all conditioning layers and polysaccharides were not present in appreciable concentrations; the proportion of nitrogen which may be due to DNA was lower than 15%.
26769222	4	8	attach	present	842:848	arg2	polysaccharides					817:831	polysaccharides	817:831	polysaccharides	817:831	Proteins or peptides were found to be a major constituent of all conditioning layers and polysaccharides were not present in appreciable concentrations; the proportion of nitrogen which may be due to DNA was lower than 15%.
26769222	4	8	attach	present	842:848	arg1	concentrations					865:878	appreciable concentrations	853:878	appreciable concentrations; the proportion of nitrogen which may be due to DNA was lower than 15%	853:949	Proteins or peptides were found to be a major constituent of all conditioning layers and polysaccharides were not present in appreciable concentrations; the proportion of nitrogen which may be due to DNA was lower than 15%.
26769222	0	9	theme	bacterial	111:119	arg1	cultures					121:128	bacterial cultures	111:128	bacterial cultures	111:128	Proteins dominate in the surface layers formed on materials exposed to extracellular polymeric substances from bacterial cultures.
26769222	5	10	theme	significant	965:975	arg1	difference					977:986	no significant difference	962:986	no significant difference between the compositions of the adlayers formed from different conditioning solutions	962:1072	There was no significant difference between the compositions of the adlayers formed from different conditioning solutions, except for the adlayers produced with tightly bound EPS extracted from D. alaskensis.
26769222	1	11	theme	conditioning	172:183	arg1	layers					185:190	the surface conditioning layers	160:190	the surface conditioning layers formed by different types of solutions (from isolated EPS to whole culture media), involving different bacterial strains relevant for biocorrosion	160:337	The chemical compositions of the surface conditioning layers formed by different types of solutions (from isolated EPS to whole culture media), involving different bacterial strains relevant for biocorrosion were compared, as they may influence the initial step in biofilm formation.
26769222	4	12	from	concentrations	865:878	arg1	present					842:848	present	842:848	present	842:848	Proteins or peptides were found to be a major constituent of all conditioning layers and polysaccharides were not present in appreciable concentrations; the proportion of nitrogen which may be due to DNA was lower than 15%.
26769222	2	13	dep	substrata	425:433	arg1	polystyrene					436:446	polystyrene	436:446	polystyrene	436:446	Different substrata (polystyrene, glass, steel) were conditioned and analyzed by X-ray photoelectron spectroscopy.
26769222	2	13	dep	substrata	425:433	arg1	steel					456:460	steel	456:460	steel	456:460	Different substrata (polystyrene, glass, steel) were conditioned and analyzed by X-ray photoelectron spectroscopy.
26769222	2	13	dep	substrata	425:433	arg1	glass					449:453	glass	449:453	glass	449:453	Different substrata (polystyrene, glass, steel) were conditioned and analyzed by X-ray photoelectron spectroscopy.
26769222	4	14	located	present	842:848	arg2	polysaccharides					817:831	polysaccharides	817:831	polysaccharides	817:831	Proteins or peptides were found to be a major constituent of all conditioning layers and polysaccharides were not present in appreciable concentrations; the proportion of nitrogen which may be due to DNA was lower than 15%.
26769222	4	14	located	present	842:848	arg1	concentrations					865:878	appreciable concentrations	853:878	appreciable concentrations; the proportion of nitrogen which may be due to DNA was lower than 15%	853:949	Proteins or peptides were found to be a major constituent of all conditioning layers and polysaccharides were not present in appreciable concentrations; the proportion of nitrogen which may be due to DNA was lower than 15%.
26769222	3	15	theme	Peak	530:533	arg1	decomposition					535:547	Peak decomposition	530:547	Peak decomposition	530:547	Peak decomposition and assignment were validated by correlations between independent spectral data and the ubiquitous presence of organic contaminants on inorganic substrata was taken into account.
26769222	1	16	theme	layers	185:190	arg1	compositions					144:155	The chemical compositions	131:155	The chemical compositions of the surface conditioning layers formed by different types of solutions (from isolated EPS to whole culture media), involving different bacterial strains relevant for biocorrosion	131:337	The chemical compositions of the surface conditioning layers formed by different types of solutions (from isolated EPS to whole culture media), involving different bacterial strains relevant for biocorrosion were compared, as they may influence the initial step in biofilm formation.
26769222	5	17	theme	adlayers	1020:1027	arg1	compositions					1000:1011	the compositions	996:1011	the compositions of the adlayers formed from different conditioning solutions	996:1072	There was no significant difference between the compositions of the adlayers formed from different conditioning solutions, except for the adlayers produced with tightly bound EPS extracted from D. alaskensis.
26769222	5	18	theme	bound	1121:1125	arg1	EPS					1127:1129	tightly bound EPS	1113:1129	tightly bound EPS extracted from D. alaskensis	1113:1158	There was no significant difference between the compositions of the adlayers formed from different conditioning solutions, except for the adlayers produced with tightly bound EPS extracted from D. alaskensis.
26769222	5	19	theme	different	1041:1049	arg1	solutions					1064:1072	different conditioning solutions	1041:1072	different conditioning solutions	1041:1072	There was no significant difference between the compositions of the adlayers formed from different conditioning solutions, except for the adlayers produced with tightly bound EPS extracted from D. alaskensis.
26769222	3	20	theme	contaminants	668:679	arg1	presence					648:655	the ubiquitous presence	633:655	the ubiquitous presence of organic contaminants on inorganic substrata	633:702	Peak decomposition and assignment were validated by correlations between independent spectral data and the ubiquitous presence of organic contaminants on inorganic substrata was taken into account.
26769222	4	21	dep	concentrations	865:878	arg1	due					921:923	due	921:923	due	921:923	Proteins or peptides were found to be a major constituent of all conditioning layers and polysaccharides were not present in appreciable concentrations; the proportion of nitrogen which may be due to DNA was lower than 15%.
26769222	4	21	dep	concentrations	865:878	arg1	lower					936:940	lower	936:940	lower	936:940	Proteins or peptides were found to be a major constituent of all conditioning layers and polysaccharides were not present in appreciable concentrations; the proportion of nitrogen which may be due to DNA was lower than 15%.
26769222	4	21	dep	concentrations	865:878	arg1	proportion					885:894	the proportion	881:894	the proportion of nitrogen which may be due to DNA	881:930	Proteins or peptides were found to be a major constituent of all conditioning layers and polysaccharides were not present in appreciable concentrations; the proportion of nitrogen which may be due to DNA was lower than 15%.
26769222	1	22	theme	different	202:210	arg1	types					212:216	different types	202:216	different types of solutions (from isolated EPS to whole culture media), involving different bacterial strains relevant for biocorrosion	202:337	The chemical compositions of the surface conditioning layers formed by different types of solutions (from isolated EPS to whole culture media), involving different bacterial strains relevant for biocorrosion were compared, as they may influence the initial step in biofilm formation.
26769222	3	23	theme	organic	660:666	arg1	contaminants					668:679	organic contaminants	660:679	organic contaminants	660:679	Peak decomposition and assignment were validated by correlations between independent spectral data and the ubiquitous presence of organic contaminants on inorganic substrata was taken into account.
26769222	1	24	theme	different	285:293	arg1	strains					305:311	different bacterial strains	285:311	different bacterial strains relevant for biocorrosion	285:337	The chemical compositions of the surface conditioning layers formed by different types of solutions (from isolated EPS to whole culture media), involving different bacterial strains relevant for biocorrosion were compared, as they may influence the initial step in biofilm formation.
26769222	1	25	theme	initial	380:386	arg1	step					388:391	the initial step	376:391	the initial step in biofilm formation	376:412	The chemical compositions of the surface conditioning layers formed by different types of solutions (from isolated EPS to whole culture media), involving different bacterial strains relevant for biocorrosion were compared, as they may influence the initial step in biofilm formation.
26769222	3	26	theme	independent	603:613	arg1	data					624:627	independent spectral data	603:627	independent spectral data	603:627	Peak decomposition and assignment were validated by correlations between independent spectral data and the ubiquitous presence of organic contaminants on inorganic substrata was taken into account.
26769222	0	27	theme	surface	25:31	arg1	layers					33:38	the surface layers	21:38	the surface layers formed on materials exposed to extracellular polymeric substances from bacterial cultures	21:128	Proteins dominate in the surface layers formed on materials exposed to extracellular polymeric substances from bacterial cultures.
26769222	1	28	theme	bacterial	295:303	arg1	strains					305:311	different bacterial strains	285:311	different bacterial strains relevant for biocorrosion	285:337	The chemical compositions of the surface conditioning layers formed by different types of solutions (from isolated EPS to whole culture media), involving different bacterial strains relevant for biocorrosion were compared, as they may influence the initial step in biofilm formation.
26769222	4	29	theme	major	768:772	arg1	constituent					774:784	a major constituent	766:784	a major constituent of all conditioning layers	766:811	Proteins or peptides were found to be a major constituent of all conditioning layers and polysaccharides were not present in appreciable concentrations; the proportion of nitrogen which may be due to DNA was lower than 15%.
26769222	4	29	theme	major	768:772	arg1	peptides					740:747	peptides	740:747	peptides	740:747	Proteins or peptides were found to be a major constituent of all conditioning layers and polysaccharides were not present in appreciable concentrations; the proportion of nitrogen which may be due to DNA was lower than 15%.
26769222	4	29	theme	major	768:772	arg1	Proteins					728:735	Proteins	728:735	Proteins	728:735	Proteins or peptides were found to be a major constituent of all conditioning layers and polysaccharides were not present in appreciable concentrations; the proportion of nitrogen which may be due to DNA was lower than 15%.
26769222	3	30	theme	spectral	615:622	arg1	data					624:627	independent spectral data	603:627	independent spectral data	603:627	Peak decomposition and assignment were validated by correlations between independent spectral data and the ubiquitous presence of organic contaminants on inorganic substrata was taken into account.
26769222	1	31	theme	solutions	221:229	arg1	types					212:216	different types	202:216	different types of solutions (from isolated EPS to whole culture media), involving different bacterial strains relevant for biocorrosion	202:337	The chemical compositions of the surface conditioning layers formed by different types of solutions (from isolated EPS to whole culture media), involving different bacterial strains relevant for biocorrosion were compared, as they may influence the initial step in biofilm formation.
26769222	3	32	from	presence	648:655	arg1	substrata					694:702	inorganic substrata	684:702	inorganic substrata	684:702	Peak decomposition and assignment were validated by correlations between independent spectral data and the ubiquitous presence of organic contaminants on inorganic substrata was taken into account.
26769222	2	33	theme	photoelectron	502:514	arg1	spectroscopy					516:527	X-ray photoelectron spectroscopy	496:527	X-ray photoelectron spectroscopy	496:527	Different substrata (polystyrene, glass, steel) were conditioned and analyzed by X-ray photoelectron spectroscopy.
26769222	4	34	from	present	842:848	arg1	concentrations					865:878	appreciable concentrations	853:878	appreciable concentrations; the proportion of nitrogen which may be due to DNA was lower than 15%	853:949	Proteins or peptides were found to be a major constituent of all conditioning layers and polysaccharides were not present in appreciable concentrations; the proportion of nitrogen which may be due to DNA was lower than 15%.
26769222	3	35	attach	presence	648:655	arg1	substrata					694:702	inorganic substrata	684:702	inorganic substrata	684:702	Peak decomposition and assignment were validated by correlations between independent spectral data and the ubiquitous presence of organic contaminants on inorganic substrata was taken into account.
26769222	3	35	attach	presence	648:655	arg2	contaminants					668:679	organic contaminants	660:679	organic contaminants	660:679	Peak decomposition and assignment were validated by correlations between independent spectral data and the ubiquitous presence of organic contaminants on inorganic substrata was taken into account.
26769222	1	36	theme	relevant	313:320	arg1	strains					305:311	different bacterial strains	285:311	different bacterial strains relevant for biocorrosion	285:337	The chemical compositions of the surface conditioning layers formed by different types of solutions (from isolated EPS to whole culture media), involving different bacterial strains relevant for biocorrosion were compared, as they may influence the initial step in biofilm formation.
26769222	1	37	from	EPS	246:248	arg1	types					212:216	different types	202:216	different types of solutions (from isolated EPS to whole culture media), involving different bacterial strains relevant for biocorrosion	202:337	The chemical compositions of the surface conditioning layers formed by different types of solutions (from isolated EPS to whole culture media), involving different bacterial strains relevant for biocorrosion were compared, as they may influence the initial step in biofilm formation.
26769222	1	37	from	EPS	246:248	arg1	solutions					221:229	solutions	221:229	solutions (from isolated EPS to whole culture media)	221:272	The chemical compositions of the surface conditioning layers formed by different types of solutions (from isolated EPS to whole culture media), involving different bacterial strains relevant for biocorrosion were compared, as they may influence the initial step in biofilm formation.
26769222	1	38	theme	biofilm	396:402	arg1	formation					404:412	biofilm formation	396:412	biofilm formation	396:412	The chemical compositions of the surface conditioning layers formed by different types of solutions (from isolated EPS to whole culture media), involving different bacterial strains relevant for biocorrosion were compared, as they may influence the initial step in biofilm formation.
26769222	4	39	theme	appreciable	853:863	arg1	concentrations					865:878	appreciable concentrations	853:878	appreciable concentrations; the proportion of nitrogen which may be due to DNA was lower than 15%	853:949	Proteins or peptides were found to be a major constituent of all conditioning layers and polysaccharides were not present in appreciable concentrations; the proportion of nitrogen which may be due to DNA was lower than 15%.
26769222	5	40	theme	conditioning	1051:1062	arg1	solutions					1064:1072	different conditioning solutions	1041:1072	different conditioning solutions	1041:1072	There was no significant difference between the compositions of the adlayers formed from different conditioning solutions, except for the adlayers produced with tightly bound EPS extracted from D. alaskensis.
26769222	4	41	theme	layers	806:811	arg1	constituent					774:784	a major constituent	766:784	a major constituent of all conditioning layers	766:811	Proteins or peptides were found to be a major constituent of all conditioning layers and polysaccharides were not present in appreciable concentrations; the proportion of nitrogen which may be due to DNA was lower than 15%.
26769222	4	41	theme	layers	806:811	arg1	peptides					740:747	peptides	740:747	peptides	740:747	Proteins or peptides were found to be a major constituent of all conditioning layers and polysaccharides were not present in appreciable concentrations; the proportion of nitrogen which may be due to DNA was lower than 15%.
26769222	4	41	theme	layers	806:811	arg1	Proteins					728:735	Proteins	728:735	Proteins	728:735	Proteins or peptides were found to be a major constituent of all conditioning layers and polysaccharides were not present in appreciable concentrations; the proportion of nitrogen which may be due to DNA was lower than 15%.
26769222	1	42	from	types	212:216	arg1	EPS					246:248	isolated EPS	237:248	isolated EPS to whole culture media	237:271	The chemical compositions of the surface conditioning layers formed by different types of solutions (from isolated EPS to whole culture media), involving different bacterial strains relevant for biocorrosion were compared, as they may influence the initial step in biofilm formation.
26769222	3	43	theme	ubiquitous	637:646	arg1	presence					648:655	the ubiquitous presence	633:655	the ubiquitous presence of organic contaminants on inorganic substrata	633:702	Peak decomposition and assignment were validated by correlations between independent spectral data and the ubiquitous presence of organic contaminants on inorganic substrata was taken into account.
26769222	1	44	theme	chemical	135:142	arg1	compositions					144:155	The chemical compositions	131:155	The chemical compositions of the surface conditioning layers formed by different types of solutions (from isolated EPS to whole culture media), involving different bacterial strains relevant for biocorrosion	131:337	The chemical compositions of the surface conditioning layers formed by different types of solutions (from isolated EPS to whole culture media), involving different bacterial strains relevant for biocorrosion were compared, as they may influence the initial step in biofilm formation.
26769222	2	45	theme	X-ray	496:500	arg1	spectroscopy					516:527	X-ray photoelectron spectroscopy	496:527	X-ray photoelectron spectroscopy	496:527	Different substrata (polystyrene, glass, steel) were conditioned and analyzed by X-ray photoelectron spectroscopy.
26769222	3	46	theme	inorganic	684:692	arg1	substrata					694:702	inorganic substrata	684:702	inorganic substrata	684:702	Peak decomposition and assignment were validated by correlations between independent spectral data and the ubiquitous presence of organic contaminants on inorganic substrata was taken into account.
26769222	2	47	theme	Different	415:423	arg1	substrata					425:433	Different substrata	415:433	Different substrata (polystyrene, glass, steel)	415:461	Different substrata (polystyrene, glass, steel) were conditioned and analyzed by X-ray photoelectron spectroscopy.
26769222	1	48	theme	isolated	237:244	arg1	EPS					246:248	isolated EPS	237:248	isolated EPS to whole culture media	237:271	The chemical compositions of the surface conditioning layers formed by different types of solutions (from isolated EPS to whole culture media), involving different bacterial strains relevant for biocorrosion were compared, as they may influence the initial step in biofilm formation.
26769222	0	49	from	cultures	121:128	arg1	substances					95:104	extracellular polymeric substances	71:104	extracellular polymeric substances from bacterial cultures	71:128	Proteins dominate in the surface layers formed on materials exposed to extracellular polymeric substances from bacterial cultures.
24288113	5	0	theme	biological	765:774	arg1	recognition					804:814	molecular recognition	794:814	molecular recognition	794:814	Such a system might allow selective biological functions such as molecular recognition and specific catalysis and also for probing the detailed genetic and molecular mechanisms of life.
24288113	5	0	theme	biological	765:774	arg1	catalysis					829:837	specific catalysis	820:837	specific catalysis	820:837	Such a system might allow selective biological functions such as molecular recognition and specific catalysis and also for probing the detailed genetic and molecular mechanisms of life.
24288113	5	0	theme	biological	765:774	arg1	functions					776:784	selective biological functions	755:784	selective biological functions such as molecular recognition and specific catalysis	755:837	Such a system might allow selective biological functions such as molecular recognition and specific catalysis and also for probing the detailed genetic and molecular mechanisms of life.
24288113	6	1	theme	composite	923:931	arg1	hydrogel					933:940	A BC-CP composite hydrogel	915:940	A BC-CP composite hydrogel	915:940	A BC-CP composite hydrogel could then lead to a biology-device interface.
24288113	10	2	theme	skin	1362:1365	arg1	fibroblasts					1367:1377	human normal skin fibroblasts	1349:1377	human normal skin fibroblasts	1349:1377	BC hydrogels were found to be able to support the growth, spreading and migration of human normal skin fibroblasts without causing any cytotoxic effect on the cells in the cell culture.
24288113	5	3	theme	life	909:912	arg1	mechanisms					895:904	the detailed genetic and molecular mechanisms	860:904	the detailed genetic and molecular mechanisms of life	860:912	Such a system might allow selective biological functions such as molecular recognition and specific catalysis and also for probing the detailed genetic and molecular mechanisms of life.
24288113	8	4	theme	voltage	1159:1165	arg1	changes					1167:1173	voltage changes	1159:1173	voltage changes	1159:1173	BC-PAni and BC-PPy respond to voltage changes.
24288113	6	5	theme	BC-CP	917:921	arg1	hydrogel					933:940	A BC-CP composite hydrogel	915:940	A BC-CP composite hydrogel	915:940	A BC-CP composite hydrogel could then lead to a biology-device interface.
24288113	11	6	theme	biphasic	1491:1498	arg1	hydrogels					1506:1514	biphasic Janus hydrogels	1491:1514	biphasic Janus hydrogels which integrate electroactivity with biocompatibility, and might provide a biology-device interface to produce implantable devices for personalized and regenerative medicine	1491:1688	These double network BC-CP hydrogels are biphasic Janus hydrogels which integrate electroactivity with biocompatibility, and might provide a biology-device interface to produce implantable devices for personalized and regenerative medicine.
24288113	11	6	theme	biphasic	1491:1498	arg1	hydrogels					1477:1485	These double network BC-CP hydrogels	1450:1485	These double network BC-CP hydrogels	1450:1485	These double network BC-CP hydrogels are biphasic Janus hydrogels which integrate electroactivity with biocompatibility, and might provide a biology-device interface to produce implantable devices for personalized and regenerative medicine.
24288113	4	7	theme	bioadhesion	686:696	arg1	properties					717:726	good biocompatibility, biodegradability, bioadhesion and mass transport properties	645:726	good biocompatibility, biodegradability, bioadhesion and mass transport properties	645:726	The electroconductivity provides the composites with capabilities for voltage and current response, and the BC hydrogel layer provides good biocompatibility, biodegradability, bioadhesion and mass transport properties.
24288113	10	8	theme	fibroblasts	1367:1377	arg1	migration					1336:1344	migration	1336:1344	migration	1336:1344	BC hydrogels were found to be able to support the growth, spreading and migration of human normal skin fibroblasts without causing any cytotoxic effect on the cells in the cell culture.
24288113	10	8	theme	fibroblasts	1367:1377	arg1	spreading					1322:1330	spreading	1322:1330	spreading	1322:1330	BC hydrogels were found to be able to support the growth, spreading and migration of human normal skin fibroblasts without causing any cytotoxic effect on the cells in the cell culture.
24288113	10	8	theme	fibroblasts	1367:1377	arg1	growth					1314:1319	growth	1314:1319	growth	1314:1319	BC hydrogels were found to be able to support the growth, spreading and migration of human normal skin fibroblasts without causing any cytotoxic effect on the cells in the cell culture.
24288113	10	9	theme	cytotoxic	1399:1407	arg1	effect					1409:1414	any cytotoxic effect	1395:1414	any cytotoxic effect on the cells in the cell culture	1395:1447	BC hydrogels were found to be able to support the growth, spreading and migration of human normal skin fibroblasts without causing any cytotoxic effect on the cells in the cell culture.
24288113	11	10	theme	personalized	1651:1662	arg1	medicine					1681:1688	personalized and regenerative medicine	1651:1688	medicine	1681:1688	These double network BC-CP hydrogels are biphasic Janus hydrogels which integrate electroactivity with biocompatibility, and might provide a biology-device interface to produce implantable devices for personalized and regenerative medicine.
24288113	10	11	theme	cell	1436:1439	arg1	culture					1441:1447	the cell culture	1432:1447	the cell culture	1432:1447	BC hydrogels were found to be able to support the growth, spreading and migration of human normal skin fibroblasts without causing any cytotoxic effect on the cells in the cell culture.
24288113	3	12	contain	have	412:415	arg1	structures					376:385	The structures	372:385	The structures of these composites	372:405	The structures of these composites thus have double networks, one of which is a layer of electroactive hydrogels combined with BC and CP.
24288113	3	12	contain	have	412:415	arg2	networks					424:431	double networks	417:431	double networks	417:431	The structures of these composites thus have double networks, one of which is a layer of electroactive hydrogels combined with BC and CP.
24288113	4	13	with	composites	547:556	arg1	capabilities					563:574	capabilities	563:574	capabilities	563:574	The electroconductivity provides the composites with capabilities for voltage and current response, and the BC hydrogel layer provides good biocompatibility, biodegradability, bioadhesion and mass transport properties.
24288113	4	14	theme	voltage	580:586	arg1	response					600:607	voltage and current response	580:607	voltage and current response	580:607	The electroconductivity provides the composites with capabilities for voltage and current response, and the BC hydrogel layer provides good biocompatibility, biodegradability, bioadhesion and mass transport properties.
24288113	4	15	theme	transport	707:715	arg1	properties					717:726	good biocompatibility, biodegradability, bioadhesion and mass transport properties	645:726	good biocompatibility, biodegradability, bioadhesion and mass transport properties	645:726	The electroconductivity provides the composites with capabilities for voltage and current response, and the BC hydrogel layer provides good biocompatibility, biodegradability, bioadhesion and mass transport properties.
24288113	7	16	theme	electroactive	1105:1117	arg1	property					1119:1126	the composite hydrogels' electroactive property	1080:1126	the composite hydrogels' electroactive property	1080:1126	Cyclic voltammetry and electrochemical impedance spectroscopy (EIS) are used here to study the composite hydrogels' electroactive property.
24288113	9	17	theme	electrochemical	1213:1227	arg1	signals					1229:1235	electrochemical signals	1213:1235	electrochemical signals for analysis or detection	1213:1261	This provides a mechanism to amplify electrochemical signals for analysis or detection.
24288113	2	18	theme	polymer	298:304	arg1	deposited					311:319	bacterial cellulose (BC) and conducting polymer (CP) deposited	258:319	bacterial cellulose (BC) and conducting polymer (CP) deposited on the BC hydrogel surface	258:346	In this study, electroactive hydrogels have been produced using bacterial cellulose (BC) and conducting polymer (CP) deposited on the BC hydrogel surface to cover the BC fibers.
24288113	11	19	with	electroactivity	1532:1546	arg1	biocompatibility					1553:1568	biocompatibility	1553:1568	biocompatibility	1553:1568	These double network BC-CP hydrogels are biphasic Janus hydrogels which integrate electroactivity with biocompatibility, and might provide a biology-device interface to produce implantable devices for personalized and regenerative medicine.
24288113	4	20	theme	good	645:648	arg1	properties					717:726	good biocompatibility, biodegradability, bioadhesion and mass transport properties	645:726	good biocompatibility, biodegradability, bioadhesion and mass transport properties	645:726	The electroconductivity provides the composites with capabilities for voltage and current response, and the BC hydrogel layer provides good biocompatibility, biodegradability, bioadhesion and mass transport properties.
24288113	7	21	used	used	1061:1064	arg2	EIS					1052:1054	EIS	1052:1054	EIS	1052:1054	Cyclic voltammetry and electrochemical impedance spectroscopy (EIS) are used here to study the composite hydrogels' electroactive property.
24288113	7	21	used	used	1061:1064	arg2	spectroscopy					1038:1049	electrochemical impedance spectroscopy	1012:1049	electrochemical impedance spectroscopy (EIS)	1012:1055	Cyclic voltammetry and electrochemical impedance spectroscopy (EIS) are used here to study the composite hydrogels' electroactive property.
24288113	7	21	used	used	1061:1064	arg2	voltammetry					996:1006	Cyclic voltammetry	989:1006	Cyclic voltammetry	989:1006	Cyclic voltammetry and electrochemical impedance spectroscopy (EIS) are used here to study the composite hydrogels' electroactive property.
24288113	11	22	theme	biology-device	1591:1604	arg1	interface					1606:1614	a biology-device interface	1589:1614	a biology-device interface	1589:1614	These double network BC-CP hydrogels are biphasic Janus hydrogels which integrate electroactivity with biocompatibility, and might provide a biology-device interface to produce implantable devices for personalized and regenerative medicine.
24288113	2	23	theme	conducting	287:296	arg1	CP					307:308	CP	307:308	CP	307:308	In this study, electroactive hydrogels have been produced using bacterial cellulose (BC) and conducting polymer (CP) deposited on the BC hydrogel surface to cover the BC fibers.
24288113	2	23	theme	conducting	287:296	arg1	polymer					298:304	conducting polymer	287:304	conducting polymer (CP)	287:309	In this study, electroactive hydrogels have been produced using bacterial cellulose (BC) and conducting polymer (CP) deposited on the BC hydrogel surface to cover the BC fibers.
24288113	5	24	theme	genetic	873:879	arg1	mechanisms					895:904	the detailed genetic and molecular mechanisms	860:904	the detailed genetic and molecular mechanisms of life	860:912	Such a system might allow selective biological functions such as molecular recognition and specific catalysis and also for probing the detailed genetic and molecular mechanisms of life.
24288113	0	25	theme	network	7:13	arg1	hydrogel					35:42	Double network bacterial cellulose hydrogel	0:42	Double network bacterial cellulose hydrogel	0:42	Double network bacterial cellulose hydrogel to build a biology-device interface.
24288113	3	26	theme	double	417:422	arg1	networks					424:431	double networks	417:431	double networks	417:431	The structures of these composites thus have double networks, one of which is a layer of electroactive hydrogels combined with BC and CP.
24288113	0	27	theme	Double	0:5	arg1	hydrogel					35:42	Double network bacterial cellulose hydrogel	0:42	Double network bacterial cellulose hydrogel	0:42	Double network bacterial cellulose hydrogel to build a biology-device interface.
24288113	1	28	theme	biology-device	96:109	arg1	interface					111:119	a biology-device interface	94:119	a biology-device interface	94:119	Establishing a biology-device interface might enable the interaction between microelectronics and biotechnology.
24288113	11	29	theme	BC-CP	1471:1475	arg1	hydrogels					1506:1514	biphasic Janus hydrogels	1491:1514	biphasic Janus hydrogels which integrate electroactivity with biocompatibility, and might provide a biology-device interface to produce implantable devices for personalized and regenerative medicine	1491:1688	These double network BC-CP hydrogels are biphasic Janus hydrogels which integrate electroactivity with biocompatibility, and might provide a biology-device interface to produce implantable devices for personalized and regenerative medicine.
24288113	11	29	theme	BC-CP	1471:1475	arg1	hydrogels					1477:1485	These double network BC-CP hydrogels	1450:1485	These double network BC-CP hydrogels	1450:1485	These double network BC-CP hydrogels are biphasic Janus hydrogels which integrate electroactivity with biocompatibility, and might provide a biology-device interface to produce implantable devices for personalized and regenerative medicine.
24288113	4	30	theme	biocompatibility	650:665	arg1	properties					717:726	good biocompatibility, biodegradability, bioadhesion and mass transport properties	645:726	good biocompatibility, biodegradability, bioadhesion and mass transport properties	645:726	The electroconductivity provides the composites with capabilities for voltage and current response, and the BC hydrogel layer provides good biocompatibility, biodegradability, bioadhesion and mass transport properties.
24288113	0	31	theme	cellulose	25:33	arg1	hydrogel					35:42	Double network bacterial cellulose hydrogel	0:42	Double network bacterial cellulose hydrogel	0:42	Double network bacterial cellulose hydrogel to build a biology-device interface.
24288113	5	32	theme	specific	820:827	arg1	catalysis					829:837	specific catalysis	820:837	specific catalysis	820:837	Such a system might allow selective biological functions such as molecular recognition and specific catalysis and also for probing the detailed genetic and molecular mechanisms of life.
24288113	4	33	theme	BC	618:619	arg1	layer					630:634	the BC hydrogel layer	614:634	the BC hydrogel layer	614:634	The electroconductivity provides the composites with capabilities for voltage and current response, and the BC hydrogel layer provides good biocompatibility, biodegradability, bioadhesion and mass transport properties.
24288113	0	34	theme	bacterial	15:23	arg1	hydrogel					35:42	Double network bacterial cellulose hydrogel	0:42	Double network bacterial cellulose hydrogel	0:42	Double network bacterial cellulose hydrogel to build a biology-device interface.
24288113	6	35	theme	biology-device	963:976	arg1	interface					978:986	a biology-device interface	961:986	a biology-device interface	961:986	A BC-CP composite hydrogel could then lead to a biology-device interface.
24288113	7	36	theme	composite	1084:1092	arg1	property					1119:1126	the composite hydrogels' electroactive property	1080:1126	the composite hydrogels' electroactive property	1080:1126	Cyclic voltammetry and electrochemical impedance spectroscopy (EIS) are used here to study the composite hydrogels' electroactive property.
24288113	11	37	theme	network	1463:1469	arg1	hydrogels					1506:1514	biphasic Janus hydrogels	1491:1514	biphasic Janus hydrogels which integrate electroactivity with biocompatibility, and might provide a biology-device interface to produce implantable devices for personalized and regenerative medicine	1491:1688	These double network BC-CP hydrogels are biphasic Janus hydrogels which integrate electroactivity with biocompatibility, and might provide a biology-device interface to produce implantable devices for personalized and regenerative medicine.
24288113	11	37	theme	network	1463:1469	arg1	hydrogels					1477:1485	These double network BC-CP hydrogels	1450:1485	These double network BC-CP hydrogels	1450:1485	These double network BC-CP hydrogels are biphasic Janus hydrogels which integrate electroactivity with biocompatibility, and might provide a biology-device interface to produce implantable devices for personalized and regenerative medicine.
24288113	11	38	theme	double	1456:1461	arg1	hydrogels					1506:1514	biphasic Janus hydrogels	1491:1514	biphasic Janus hydrogels which integrate electroactivity with biocompatibility, and might provide a biology-device interface to produce implantable devices for personalized and regenerative medicine	1491:1688	These double network BC-CP hydrogels are biphasic Janus hydrogels which integrate electroactivity with biocompatibility, and might provide a biology-device interface to produce implantable devices for personalized and regenerative medicine.
24288113	11	38	theme	double	1456:1461	arg1	hydrogels					1477:1485	These double network BC-CP hydrogels	1450:1485	These double network BC-CP hydrogels	1450:1485	These double network BC-CP hydrogels are biphasic Janus hydrogels which integrate electroactivity with biocompatibility, and might provide a biology-device interface to produce implantable devices for personalized and regenerative medicine.
24288113	4	39	theme	mass	702:705	arg1	properties					717:726	good biocompatibility, biodegradability, bioadhesion and mass transport properties	645:726	good biocompatibility, biodegradability, bioadhesion and mass transport properties	645:726	The electroconductivity provides the composites with capabilities for voltage and current response, and the BC hydrogel layer provides good biocompatibility, biodegradability, bioadhesion and mass transport properties.
24288113	2	40	theme	cellulose	268:276	arg1	deposited					311:319	bacterial cellulose (BC) and conducting polymer (CP) deposited	258:319	bacterial cellulose (BC) and conducting polymer (CP) deposited on the BC hydrogel surface	258:346	In this study, electroactive hydrogels have been produced using bacterial cellulose (BC) and conducting polymer (CP) deposited on the BC hydrogel surface to cover the BC fibers.
24288113	3	41	theme	networks	424:431	arg1	layer					452:456	a layer	450:456	a layer of electroactive hydrogels combined with BC and CP	450:507	The structures of these composites thus have double networks, one of which is a layer of electroactive hydrogels combined with BC and CP.
24288113	3	41	theme	networks	424:431	arg1	one					434:436	one	434:436	one	434:436	The structures of these composites thus have double networks, one of which is a layer of electroactive hydrogels combined with BC and CP.
24288113	3	41	theme	networks	424:431	arg1	networks					424:431	double networks	417:431	double networks	417:431	The structures of these composites thus have double networks, one of which is a layer of electroactive hydrogels combined with BC and CP.
24288113	5	42	theme	detailed	864:871	arg1	mechanisms					895:904	the detailed genetic and molecular mechanisms	860:904	the detailed genetic and molecular mechanisms of life	860:912	Such a system might allow selective biological functions such as molecular recognition and specific catalysis and also for probing the detailed genetic and molecular mechanisms of life.
24288113	10	43	from	effect	1409:1414	arg1	cells					1423:1427	the cells	1419:1427	the cells in the cell culture	1419:1447	BC hydrogels were found to be able to support the growth, spreading and migration of human normal skin fibroblasts without causing any cytotoxic effect on the cells in the cell culture.
24288113	2	44	theme	bacterial	258:266	arg1	BC					279:280	BC	279:280	BC	279:280	In this study, electroactive hydrogels have been produced using bacterial cellulose (BC) and conducting polymer (CP) deposited on the BC hydrogel surface to cover the BC fibers.
24288113	2	44	theme	bacterial	258:266	arg1	cellulose					268:276	bacterial cellulose	258:276	bacterial cellulose (BC)	258:281	In this study, electroactive hydrogels have been produced using bacterial cellulose (BC) and conducting polymer (CP) deposited on the BC hydrogel surface to cover the BC fibers.
24288113	10	45	theme	normal	1355:1360	arg1	fibroblasts					1367:1377	human normal skin fibroblasts	1349:1377	human normal skin fibroblasts	1349:1377	BC hydrogels were found to be able to support the growth, spreading and migration of human normal skin fibroblasts without causing any cytotoxic effect on the cells in the cell culture.
24288113	4	46	theme	hydrogel	621:628	arg1	layer					630:634	the BC hydrogel layer	614:634	the BC hydrogel layer	614:634	The electroconductivity provides the composites with capabilities for voltage and current response, and the BC hydrogel layer provides good biocompatibility, biodegradability, bioadhesion and mass transport properties.
24288113	5	47	theme	molecular	794:802	arg1	recognition					804:814	molecular recognition	794:814	molecular recognition	794:814	Such a system might allow selective biological functions such as molecular recognition and specific catalysis and also for probing the detailed genetic and molecular mechanisms of life.
24288113	5	48	theme	selective	755:763	arg1	recognition					804:814	molecular recognition	794:814	molecular recognition	794:814	Such a system might allow selective biological functions such as molecular recognition and specific catalysis and also for probing the detailed genetic and molecular mechanisms of life.
24288113	5	48	theme	selective	755:763	arg1	catalysis					829:837	specific catalysis	820:837	specific catalysis	820:837	Such a system might allow selective biological functions such as molecular recognition and specific catalysis and also for probing the detailed genetic and molecular mechanisms of life.
24288113	5	48	theme	selective	755:763	arg1	functions					776:784	selective biological functions	755:784	selective biological functions such as molecular recognition and specific catalysis	755:837	Such a system might allow selective biological functions such as molecular recognition and specific catalysis and also for probing the detailed genetic and molecular mechanisms of life.
24288113	10	49	theme	human	1349:1353	arg1	fibroblasts					1367:1377	human normal skin fibroblasts	1349:1377	human normal skin fibroblasts	1349:1377	BC hydrogels were found to be able to support the growth, spreading and migration of human normal skin fibroblasts without causing any cytotoxic effect on the cells in the cell culture.
24288113	3	50	theme	electroactive	461:473	arg1	hydrogels					475:483	electroactive hydrogels	461:483	electroactive hydrogels combined with BC and CP	461:507	The structures of these composites thus have double networks, one of which is a layer of electroactive hydrogels combined with BC and CP.
24288113	3	51	theme	composites	396:405	arg1	structures					376:385	The structures	372:385	The structures of these composites	372:405	The structures of these composites thus have double networks, one of which is a layer of electroactive hydrogels combined with BC and CP.
24288113	7	52	theme	impedance	1028:1036	arg1	spectroscopy					1038:1049	electrochemical impedance spectroscopy	1012:1049	electrochemical impedance spectroscopy (EIS)	1012:1055	Cyclic voltammetry and electrochemical impedance spectroscopy (EIS) are used here to study the composite hydrogels' electroactive property.
24288113	7	52	theme	impedance	1028:1036	arg1	EIS					1052:1054	EIS	1052:1054	EIS	1052:1054	Cyclic voltammetry and electrochemical impedance spectroscopy (EIS) are used here to study the composite hydrogels' electroactive property.
24288113	3	53	theme	hydrogels	475:483	arg1	layer					452:456	a layer	450:456	a layer of electroactive hydrogels combined with BC and CP	450:507	The structures of these composites thus have double networks, one of which is a layer of electroactive hydrogels combined with BC and CP.
24288113	3	53	theme	hydrogels	475:483	arg1	one					434:436	one	434:436	one	434:436	The structures of these composites thus have double networks, one of which is a layer of electroactive hydrogels combined with BC and CP.
24288113	3	53	theme	hydrogels	475:483	arg1	networks					424:431	double networks	417:431	double networks	417:431	The structures of these composites thus have double networks, one of which is a layer of electroactive hydrogels combined with BC and CP.
24288113	11	54	theme	Janus	1500:1504	arg1	hydrogels					1506:1514	biphasic Janus hydrogels	1491:1514	biphasic Janus hydrogels which integrate electroactivity with biocompatibility, and might provide a biology-device interface to produce implantable devices for personalized and regenerative medicine	1491:1688	These double network BC-CP hydrogels are biphasic Janus hydrogels which integrate electroactivity with biocompatibility, and might provide a biology-device interface to produce implantable devices for personalized and regenerative medicine.
24288113	11	54	theme	Janus	1500:1504	arg1	hydrogels					1477:1485	These double network BC-CP hydrogels	1450:1485	These double network BC-CP hydrogels	1450:1485	These double network BC-CP hydrogels are biphasic Janus hydrogels which integrate electroactivity with biocompatibility, and might provide a biology-device interface to produce implantable devices for personalized and regenerative medicine.
24288113	11	55	theme	regenerative	1668:1679	arg1	medicine					1681:1688	personalized and regenerative medicine	1651:1688	medicine	1681:1688	These double network BC-CP hydrogels are biphasic Janus hydrogels which integrate electroactivity with biocompatibility, and might provide a biology-device interface to produce implantable devices for personalized and regenerative medicine.
24288113	4	56	theme	biodegradability	668:683	arg1	properties					717:726	good biocompatibility, biodegradability, bioadhesion and mass transport properties	645:726	good biocompatibility, biodegradability, bioadhesion and mass transport properties	645:726	The electroconductivity provides the composites with capabilities for voltage and current response, and the BC hydrogel layer provides good biocompatibility, biodegradability, bioadhesion and mass transport properties.
24288113	2	57	theme	hydrogel	331:338	arg1	surface					340:346	the BC hydrogel surface	324:346	the BC hydrogel surface	324:346	In this study, electroactive hydrogels have been produced using bacterial cellulose (BC) and conducting polymer (CP) deposited on the BC hydrogel surface to cover the BC fibers.
24288113	5	58	theme	molecular	885:893	arg1	mechanisms					895:904	the detailed genetic and molecular mechanisms	860:904	the detailed genetic and molecular mechanisms of life	860:912	Such a system might allow selective biological functions such as molecular recognition and specific catalysis and also for probing the detailed genetic and molecular mechanisms of life.
24288113	11	59	theme	implantable	1627:1637	arg1	devices					1639:1645	implantable devices	1627:1645	implantable devices for personalized and regenerative medicine	1627:1688	These double network BC-CP hydrogels are biphasic Janus hydrogels which integrate electroactivity with biocompatibility, and might provide a biology-device interface to produce implantable devices for personalized and regenerative medicine.
24288113	2	60	theme	BC	328:329	arg1	surface					340:346	the BC hydrogel surface	324:346	the BC hydrogel surface	324:346	In this study, electroactive hydrogels have been produced using bacterial cellulose (BC) and conducting polymer (CP) deposited on the BC hydrogel surface to cover the BC fibers.
24288113	4	61	theme	current	592:598	arg1	response					600:607	voltage and current response	580:607	voltage and current response	580:607	The electroconductivity provides the composites with capabilities for voltage and current response, and the BC hydrogel layer provides good biocompatibility, biodegradability, bioadhesion and mass transport properties.
24288113	2	62	theme	electroactive	209:221	arg1	hydrogels					223:231	electroactive hydrogels	209:231	electroactive hydrogels	209:231	In this study, electroactive hydrogels have been produced using bacterial cellulose (BC) and conducting polymer (CP) deposited on the BC hydrogel surface to cover the BC fibers.
24288113	7	63	theme	electrochemical	1012:1026	arg1	spectroscopy					1038:1049	electrochemical impedance spectroscopy	1012:1049	electrochemical impedance spectroscopy (EIS)	1012:1055	Cyclic voltammetry and electrochemical impedance spectroscopy (EIS) are used here to study the composite hydrogels' electroactive property.
24288113	7	63	theme	electrochemical	1012:1026	arg1	EIS					1052:1054	EIS	1052:1054	EIS	1052:1054	Cyclic voltammetry and electrochemical impedance spectroscopy (EIS) are used here to study the composite hydrogels' electroactive property.
24288113	10	64	from	cells	1423:1427	arg1	culture					1441:1447	the cell culture	1432:1447	the cell culture	1432:1447	BC hydrogels were found to be able to support the growth, spreading and migration of human normal skin fibroblasts without causing any cytotoxic effect on the cells in the cell culture.
24288113	10	65	theme	BC	1264:1265	arg1	hydrogels					1267:1275	BC hydrogels	1264:1275	BC hydrogels	1264:1275	BC hydrogels were found to be able to support the growth, spreading and migration of human normal skin fibroblasts without causing any cytotoxic effect on the cells in the cell culture.
24288113	0	66	theme	biology-device	55:68	arg1	interface					70:78	a biology-device interface	53:78	a biology-device interface	53:78	Double network bacterial cellulose hydrogel to build a biology-device interface.
24288113	7	67	theme	Cyclic	989:994	arg1	voltammetry					996:1006	Cyclic voltammetry	989:1006	Cyclic voltammetry	989:1006	Cyclic voltammetry and electrochemical impedance spectroscopy (EIS) are used here to study the composite hydrogels' electroactive property.
24288113	2	68	theme	BC	361:362	arg1	fibers					364:369	the BC fibers	357:369	the BC fibers	357:369	In this study, electroactive hydrogels have been produced using bacterial cellulose (BC) and conducting polymer (CP) deposited on the BC hydrogel surface to cover the BC fibers.
24288113	2	69	from	deposited	311:319	arg1	surface					340:346	the BC hydrogel surface	324:346	the BC hydrogel surface	324:346	In this study, electroactive hydrogels have been produced using bacterial cellulose (BC) and conducting polymer (CP) deposited on the BC hydrogel surface to cover the BC fibers.
29169470	13	0	with	Patients	2334:2341	arg1	Type					2387:2390	Acute Heparin-induced Thrombocytopenia Type II	2348:2393	Acute Heparin-induced Thrombocytopenia Type II	2348:2393	(Retrospective Registry of Patients With Acute Heparin-induced Thrombocytopenia Type II; NCT01304238).
29169470	7	1	theme	patients	1203:1210	arg1	patients					1203:1210	patients	1203:1210	patients treated with approved alternative anticoagulation	1203:1260	The composite endpoint of HIT-specific complications (thromboembolic events, amputation, skin necrosis) occurred in 11.7% of patients treated with approved alternative anticoagulation and in 0.0% of fondaparinux-treated patients.
29169470	7	1	theme	patients	1203:1210	arg1	%					1198:1198	11.7%	1194:1198	11.7% of patients treated with approved alternative anticoagulation	1194:1260	The composite endpoint of HIT-specific complications (thromboembolic events, amputation, skin necrosis) occurred in 11.7% of patients treated with approved alternative anticoagulation and in 0.0% of fondaparinux-treated patients.
29169470	3	2	dep	OBJECTIVES	336:345	arg1	sought					359:364	sought	359:364	sought to investigate the safety and efficacy of the different anticoagulants for treating HIT	359:452	OBJECTIVES The authors sought to investigate the safety and efficacy of the different anticoagulants for treating HIT.
29169470	10	3	theme	patients	1692:1699	arg1	%					1689:1689	38.0%	1685:1689	38.0%	1685:1689	Post hoc analysis of clinical and laboratory features confirmed "true" HIT in at least 74 of 195 (38.0%) patients; 35 of 74 (47.3%) were treated with fondaparinux.
29169470	10	3	theme	patients	1692:1699	arg1	74					1674:1675	74	1674:1675	74	1674:1675	Post hoc analysis of clinical and laboratory features confirmed "true" HIT in at least 74 of 195 (38.0%) patients; 35 of 74 (47.3%) were treated with fondaparinux.
29169470	10	3	theme	patients	1692:1699	arg1	patients					1692:1699	195 (38.0%) patients	1680:1699	195 (38.0%) patients	1680:1699	Post hoc analysis of clinical and laboratory features confirmed "true" HIT in at least 74 of 195 (38.0%) patients; 35 of 74 (47.3%) were treated with fondaparinux.
29169470	10	4	theme	hoc	1592:1594	arg1	analysis					1596:1603	Post hoc analysis	1587:1603	Post hoc analysis of clinical and laboratory features	1587:1639	Post hoc analysis of clinical and laboratory features confirmed "true" HIT in at least 74 of 195 (38.0%) patients; 35 of 74 (47.3%) were treated with fondaparinux.
29169470	7	5	theme	composite	1082:1090	arg1	endpoint					1092:1099	The composite endpoint	1078:1099	The composite endpoint of HIT-specific complications (thromboembolic events, amputation, skin necrosis)	1078:1180	The composite endpoint of HIT-specific complications (thromboembolic events, amputation, skin necrosis) occurred in 11.7% of patients treated with approved alternative anticoagulation and in 0.0% of fondaparinux-treated patients.
29169470	12	6	theme	randomized	1972:1981	arg1	trials					1994:1999	randomized controlled trials	1972:1999	randomized controlled trials	1972:1999	Further data from randomized controlled trials are urgently needed because lepirudin was recalled from the market; danaparoid access has been limited and is not approved in the United States; and argatroban is contraindicated in patients with impaired liver function, and activated partial thromboplastin time confounding may interfere with monitoring.
29169470	4	7	from	METHODS	455:461	arg1	study					499:503	a national, multicenter registry study	466:503	study	499:503	METHODS In a national, multicenter registry study, hospitalized patients who were diagnosed with HIT, an at least intermediate clinical HIT-risk (4Ts score ≥4 points), and received treatment with ≥1 dose of the aforementioned anticoagulants were included.
29169470	4	7	from	METHODS	455:461	arg1	patients					519:526	hospitalized patients	506:526	hospitalized patients who were diagnosed with HIT, an at least intermediate clinical HIT-risk (4Ts score ≥4 points), and received treatment with ≥1 dose of the aforementioned anticoagulants	506:694	METHODS In a national, multicenter registry study, hospitalized patients who were diagnosed with HIT, an at least intermediate clinical HIT-risk (4Ts score ≥4 points), and received treatment with ≥1 dose of the aforementioned anticoagulants were included.
29169470	1	8	theme	nonheparin	208:217	arg1	anticoagulants					219:232	the alternative nonheparin anticoagulants argatroban, lepirudin, or danaparoid	192:269	the alternative nonheparin anticoagulants argatroban, lepirudin, or danaparoid	192:269	BACKGROUND Life-threatening heparin-induced thrombocytopenia (HIT) is treated with the alternative nonheparin anticoagulants argatroban, lepirudin, or danaparoid.
29169470	1	8	theme	nonheparin	208:217	arg1	lepirudin					246:254	lepirudin	246:254	lepirudin	246:254	BACKGROUND Life-threatening heparin-induced thrombocytopenia (HIT) is treated with the alternative nonheparin anticoagulants argatroban, lepirudin, or danaparoid.
29169470	1	8	theme	nonheparin	208:217	arg1	danaparoid					260:269	danaparoid	260:269	danaparoid	260:269	BACKGROUND Life-threatening heparin-induced thrombocytopenia (HIT) is treated with the alternative nonheparin anticoagulants argatroban, lepirudin, or danaparoid.
29169470	1	8	theme	nonheparin	208:217	arg1	argatroban					234:243	argatroban	234:243	argatroban	234:243	BACKGROUND Life-threatening heparin-induced thrombocytopenia (HIT) is treated with the alternative nonheparin anticoagulants argatroban, lepirudin, or danaparoid.
29169470	9	9	from	patients	1546:1553	arg1	%					1561:1561	6.3%	1558:1561	6.3%	1558:1561	Bleeding complications occurred in alternatively anticoagulated patients and in fondaparinux-treated patients in 6.3% and 4.8%, respectively.
29169470	9	9	from	patients	1546:1553	arg1	%					1570:1570	4.8%	1567:1570	4.8%	1567:1570	Bleeding complications occurred in alternatively anticoagulated patients and in fondaparinux-treated patients in 6.3% and 4.8%, respectively.
29169470	11	10	theme	CONCLUSIONS	1751:1761	arg1	Fondaparinux					1763:1774	CONCLUSIONS Fondaparinux	1751:1774	CONCLUSIONS Fondaparinux	1751:1774	CONCLUSIONS Fondaparinux is effective and safe in suspected acute HIT; no HIT-specific complications occurred in the fondaparinux-treated patients, even among those with a high clinical HIT probability.
29169470	4	11	theme	aforementioned	666:679	arg1	anticoagulants					681:694	the aforementioned anticoagulants	662:694	the aforementioned anticoagulants	662:694	METHODS In a national, multicenter registry study, hospitalized patients who were diagnosed with HIT, an at least intermediate clinical HIT-risk (4Ts score ≥4 points), and received treatment with ≥1 dose of the aforementioned anticoagulants were included.
29169470	5	12	theme	HIT-specific	756:767	arg1	complications					769:781	HIT-specific complications	756:781	HIT-specific complications (thromboembolic venous/arterial events, amputations, recurrent/persistent thrombocytopenia, skin lesions)	756:887	Main outcome measures were the incidences of HIT-specific complications (thromboembolic venous/arterial events, amputations, recurrent/persistent thrombocytopenia, skin lesions) and bleedings.
29169470	11	13	theme	high	1923:1926	arg1	probability					1941:1951	a high clinical HIT probability	1921:1951	a high clinical HIT probability	1921:1951	CONCLUSIONS Fondaparinux is effective and safe in suspected acute HIT; no HIT-specific complications occurred in the fondaparinux-treated patients, even among those with a high clinical HIT probability.
29169470	4	14	theme	hospitalized	506:517	arg1	study					499:503	a national, multicenter registry study	466:503	study	499:503	METHODS In a national, multicenter registry study, hospitalized patients who were diagnosed with HIT, an at least intermediate clinical HIT-risk (4Ts score ≥4 points), and received treatment with ≥1 dose of the aforementioned anticoagulants were included.
29169470	4	14	theme	hospitalized	506:517	arg1	patients					519:526	hospitalized patients	506:526	hospitalized patients who were diagnosed with HIT, an at least intermediate clinical HIT-risk (4Ts score ≥4 points), and received treatment with ≥1 dose of the aforementioned anticoagulants	506:694	METHODS In a national, multicenter registry study, hospitalized patients who were diagnosed with HIT, an at least intermediate clinical HIT-risk (4Ts score ≥4 points), and received treatment with ≥1 dose of the aforementioned anticoagulants were included.
29169470	6	15	with	first-line	995:1004	arg1	danaparoid					1034:1043	danaparoid	1034:1043	danaparoid	1034:1043	RESULTS Of 195 patients, 46 (23.6%), 4 (2.1%), 61 (31.3%), and 84 (43.1%) had been treated first-line with argatroban, lepirudin, danaparoid, and fondaparinux, respectively.
29169470	6	15	with	first-line	995:1004	arg1	fondaparinux					1050:1061	fondaparinux	1050:1061	fondaparinux	1050:1061	RESULTS Of 195 patients, 46 (23.6%), 4 (2.1%), 61 (31.3%), and 84 (43.1%) had been treated first-line with argatroban, lepirudin, danaparoid, and fondaparinux, respectively.
29169470	6	15	with	first-line	995:1004	arg1	 lepirudin					1022:1031	 lepirudin	1022:1031	 lepirudin	1022:1031	RESULTS Of 195 patients, 46 (23.6%), 4 (2.1%), 61 (31.3%), and 84 (43.1%) had been treated first-line with argatroban, lepirudin, danaparoid, and fondaparinux, respectively.
29169470	6	15	with	first-line	995:1004	arg1	argatroban					1011:1020	argatroban	1011:1020	argatroban	1011:1020	RESULTS Of 195 patients, 46 (23.6%), 4 (2.1%), 61 (31.3%), and 84 (43.1%) had been treated first-line with argatroban, lepirudin, danaparoid, and fondaparinux, respectively.
29169470	10	16	theme	features	1632:1639	arg1	analysis					1596:1603	Post hoc analysis	1587:1603	Post hoc analysis of clinical and laboratory features	1587:1639	Post hoc analysis of clinical and laboratory features confirmed "true" HIT in at least 74 of 195 (38.0%) patients; 35 of 74 (47.3%) were treated with fondaparinux.
29169470	4	17	theme	national	468:475	arg1	study					499:503	a national, multicenter registry study	466:503	study	499:503	METHODS In a national, multicenter registry study, hospitalized patients who were diagnosed with HIT, an at least intermediate clinical HIT-risk (4Ts score ≥4 points), and received treatment with ≥1 dose of the aforementioned anticoagulants were included.
29169470	4	17	theme	national	468:475	arg1	patients					519:526	hospitalized patients	506:526	hospitalized patients who were diagnosed with HIT, an at least intermediate clinical HIT-risk (4Ts score ≥4 points), and received treatment with ≥1 dose of the aforementioned anticoagulants	506:694	METHODS In a national, multicenter registry study, hospitalized patients who were diagnosed with HIT, an at least intermediate clinical HIT-risk (4Ts score ≥4 points), and received treatment with ≥1 dose of the aforementioned anticoagulants were included.
29169470	5	18	dep	complications	769:781	arg1	events					815:820	thromboembolic venous/arterial events	784:820	thromboembolic venous/arterial events	784:820	Main outcome measures were the incidences of HIT-specific complications (thromboembolic venous/arterial events, amputations, recurrent/persistent thrombocytopenia, skin lesions) and bleedings.
29169470	5	18	dep	complications	769:781	arg1	lesions					880:886	skin lesions	875:886	skin lesions	875:886	Main outcome measures were the incidences of HIT-specific complications (thromboembolic venous/arterial events, amputations, recurrent/persistent thrombocytopenia, skin lesions) and bleedings.
29169470	5	18	dep	complications	769:781	arg1	amputations					823:833	amputations	823:833	amputations	823:833	Main outcome measures were the incidences of HIT-specific complications (thromboembolic venous/arterial events, amputations, recurrent/persistent thrombocytopenia, skin lesions) and bleedings.
29169470	5	18	dep	complications	769:781	arg1	thrombocytopenia					857:872	recurrent/persistent thrombocytopenia	836:872	recurrent/persistent thrombocytopenia	836:872	Main outcome measures were the incidences of HIT-specific complications (thromboembolic venous/arterial events, amputations, recurrent/persistent thrombocytopenia, skin lesions) and bleedings.
29169470	4	19	theme	multicenter	478:488	arg1	study					499:503	a national, multicenter registry study	466:503	study	499:503	METHODS In a national, multicenter registry study, hospitalized patients who were diagnosed with HIT, an at least intermediate clinical HIT-risk (4Ts score ≥4 points), and received treatment with ≥1 dose of the aforementioned anticoagulants were included.
29169470	4	19	theme	multicenter	478:488	arg1	patients					519:526	hospitalized patients	506:526	hospitalized patients who were diagnosed with HIT, an at least intermediate clinical HIT-risk (4Ts score ≥4 points), and received treatment with ≥1 dose of the aforementioned anticoagulants	506:694	METHODS In a national, multicenter registry study, hospitalized patients who were diagnosed with HIT, an at least intermediate clinical HIT-risk (4Ts score ≥4 points), and received treatment with ≥1 dose of the aforementioned anticoagulants were included.
29169470	11	20	theme	HIT	1937:1939	arg1	probability					1941:1951	a high clinical HIT probability	1921:1951	a high clinical HIT probability	1921:1951	CONCLUSIONS Fondaparinux is effective and safe in suspected acute HIT; no HIT-specific complications occurred in the fondaparinux-treated patients, even among those with a high clinical HIT probability.
29169470	12	21	theme	thromboplastin	2244:2257	arg1	confounding					2264:2274	activated partial thromboplastin time confounding	2226:2274	activated partial thromboplastin time confounding	2226:2274	Further data from randomized controlled trials are urgently needed because lepirudin was recalled from the market; danaparoid access has been limited and is not approved in the United States; and argatroban is contraindicated in patients with impaired liver function, and activated partial thromboplastin time confounding may interfere with monitoring.
29169470	8	22	theme	all-cause	1312:1320	arg1	rates					1344:1348	The all-cause in-hospital mortality rates	1308:1348	The all-cause in-hospital mortality rates	1308:1348	The all-cause in-hospital mortality rates were 14.4% during approved alternative anticoagulation and 0.0% during fondaparinux treatment.
29169470	8	22	theme	all-cause	1312:1320	arg1	%					1359:1359	14.4%	1355:1359	14.4% during approved alternative anticoagulation	1355:1403	The all-cause in-hospital mortality rates were 14.4% during approved alternative anticoagulation and 0.0% during fondaparinux treatment.
29169470	0	23	theme	Heparin-Induced	75:89	arg1	Thrombocytopenia					91:106	Heparin-Induced Thrombocytopenia	75:106	Heparin-Induced Thrombocytopenia	75:106	Use of Fondaparinux Off-Label or Approved Anticoagulants for Management of Heparin-Induced Thrombocytopenia.
29169470	5	24	theme	outcome	716:722	arg1	measures					724:731	Main outcome measures	711:731	Main outcome measures	711:731	Main outcome measures were the incidences of HIT-specific complications (thromboembolic venous/arterial events, amputations, recurrent/persistent thrombocytopenia, skin lesions) and bleedings.
29169470	5	24	theme	outcome	716:722	arg1	incidences					742:751	the incidences	738:751	the incidences of HIT-specific complications (thromboembolic venous/arterial events, amputations, recurrent/persistent thrombocytopenia, skin lesions)	738:887	Main outcome measures were the incidences of HIT-specific complications (thromboembolic venous/arterial events, amputations, recurrent/persistent thrombocytopenia, skin lesions) and bleedings.
29169470	8	25	theme	mortality	1334:1342	arg1	rates					1344:1348	The all-cause in-hospital mortality rates	1308:1348	The all-cause in-hospital mortality rates	1308:1348	The all-cause in-hospital mortality rates were 14.4% during approved alternative anticoagulation and 0.0% during fondaparinux treatment.
29169470	8	25	theme	mortality	1334:1342	arg1	%					1359:1359	14.4%	1355:1359	14.4% during approved alternative anticoagulation	1355:1403	The all-cause in-hospital mortality rates were 14.4% during approved alternative anticoagulation and 0.0% during fondaparinux treatment.
29169470	5	26	theme	venous/arterial	799:813	arg1	events					815:820	thromboembolic venous/arterial events	784:820	thromboembolic venous/arterial events	784:820	Main outcome measures were the incidences of HIT-specific complications (thromboembolic venous/arterial events, amputations, recurrent/persistent thrombocytopenia, skin lesions) and bleedings.
29169470	5	26	theme	venous/arterial	799:813	arg1	lesions					880:886	skin lesions	875:886	skin lesions	875:886	Main outcome measures were the incidences of HIT-specific complications (thromboembolic venous/arterial events, amputations, recurrent/persistent thrombocytopenia, skin lesions) and bleedings.
29169470	5	26	theme	venous/arterial	799:813	arg1	amputations					823:833	amputations	823:833	amputations	823:833	Main outcome measures were the incidences of HIT-specific complications (thromboembolic venous/arterial events, amputations, recurrent/persistent thrombocytopenia, skin lesions) and bleedings.
29169470	5	26	theme	venous/arterial	799:813	arg1	thrombocytopenia					857:872	recurrent/persistent thrombocytopenia	836:872	recurrent/persistent thrombocytopenia	836:872	Main outcome measures were the incidences of HIT-specific complications (thromboembolic venous/arterial events, amputations, recurrent/persistent thrombocytopenia, skin lesions) and bleedings.
29169470	12	27	theme	activated	2226:2234	arg1	confounding					2264:2274	activated partial thromboplastin time confounding	2226:2274	activated partial thromboplastin time confounding	2226:2274	Further data from randomized controlled trials are urgently needed because lepirudin was recalled from the market; danaparoid access has been limited and is not approved in the United States; and argatroban is contraindicated in patients with impaired liver function, and activated partial thromboplastin time confounding may interfere with monitoring.
29169470	1	28	theme	BACKGROUND	109:118	arg1	HIT					171:173	HIT	171:173	HIT	171:173	BACKGROUND Life-threatening heparin-induced thrombocytopenia (HIT) is treated with the alternative nonheparin anticoagulants argatroban, lepirudin, or danaparoid.
29169470	1	28	theme	BACKGROUND	109:118	arg1	thrombocytopenia					153:168	BACKGROUND Life-threatening heparin-induced thrombocytopenia	109:168	BACKGROUND Life-threatening heparin-induced thrombocytopenia (HIT)	109:174	BACKGROUND Life-threatening heparin-induced thrombocytopenia (HIT) is treated with the alternative nonheparin anticoagulants argatroban, lepirudin, or danaparoid.
29169470	3	29	theme	anticoagulants	422:435	arg1	safety					385:390	safety	385:390	safety	385:390	OBJECTIVES The authors sought to investigate the safety and efficacy of the different anticoagulants for treating HIT.
29169470	3	29	theme	anticoagulants	422:435	arg1	efficacy					396:403	efficacy	396:403	efficacy	396:403	OBJECTIVES The authors sought to investigate the safety and efficacy of the different anticoagulants for treating HIT.
29169470	2	30	theme	pentasaccharide	288:302	arg1	fondaparinux					304:315	the pentasaccharide fondaparinux	284:315	the pentasaccharide fondaparinux	284:315	Frequently, the pentasaccharide fondaparinux is used off-label.
29169470	11	31	from	HIT	1817:1819	arg1	effective					1779:1787	effective	1779:1787	effective	1779:1787	CONCLUSIONS Fondaparinux is effective and safe in suspected acute HIT; no HIT-specific complications occurred in the fondaparinux-treated patients, even among those with a high clinical HIT probability.
29169470	8	32	theme	approved	1368:1375	arg1	anticoagulation					1389:1403	approved alternative anticoagulation	1368:1403	approved alternative anticoagulation	1368:1403	The all-cause in-hospital mortality rates were 14.4% during approved alternative anticoagulation and 0.0% during fondaparinux treatment.
29169470	13	33	dep	Registry	2322:2329	arg1	NCT01304238					2396:2406	NCT01304238	2396:2406	NCT01304238	2396:2406	(Retrospective Registry of Patients With Acute Heparin-induced Thrombocytopenia Type II; NCT01304238).
29169470	0	34	theme	Fondaparinux	7:18	arg1	Anticoagulants					42:55	Fondaparinux Off-Label or Approved Anticoagulants	7:55	Fondaparinux Off-Label or Approved Anticoagulants	7:55	Use of Fondaparinux Off-Label or Approved Anticoagulants for Management of Heparin-Induced Thrombocytopenia.
29169470	1	35	theme	heparin-induced	137:151	arg1	HIT					171:173	HIT	171:173	HIT	171:173	BACKGROUND Life-threatening heparin-induced thrombocytopenia (HIT) is treated with the alternative nonheparin anticoagulants argatroban, lepirudin, or danaparoid.
29169470	1	35	theme	heparin-induced	137:151	arg1	thrombocytopenia					153:168	BACKGROUND Life-threatening heparin-induced thrombocytopenia	109:168	BACKGROUND Life-threatening heparin-induced thrombocytopenia (HIT)	109:174	BACKGROUND Life-threatening heparin-induced thrombocytopenia (HIT) is treated with the alternative nonheparin anticoagulants argatroban, lepirudin, or danaparoid.
29169470	7	36	theme	fondaparinux-treated	1277:1296	arg1	patients					1298:1305	fondaparinux-treated patients	1277:1305	fondaparinux-treated patients	1277:1305	The composite endpoint of HIT-specific complications (thromboembolic events, amputation, skin necrosis) occurred in 11.7% of patients treated with approved alternative anticoagulation and in 0.0% of fondaparinux-treated patients.
29169470	1	37	dep	anticoagulants	219:232	arg1	anticoagulants					219:232	the alternative nonheparin anticoagulants argatroban, lepirudin, or danaparoid	192:269	the alternative nonheparin anticoagulants argatroban, lepirudin, or danaparoid	192:269	BACKGROUND Life-threatening heparin-induced thrombocytopenia (HIT) is treated with the alternative nonheparin anticoagulants argatroban, lepirudin, or danaparoid.
29169470	1	37	dep	anticoagulants	219:232	arg1	lepirudin					246:254	lepirudin	246:254	lepirudin	246:254	BACKGROUND Life-threatening heparin-induced thrombocytopenia (HIT) is treated with the alternative nonheparin anticoagulants argatroban, lepirudin, or danaparoid.
29169470	1	37	dep	anticoagulants	219:232	arg1	danaparoid					260:269	danaparoid	260:269	danaparoid	260:269	BACKGROUND Life-threatening heparin-induced thrombocytopenia (HIT) is treated with the alternative nonheparin anticoagulants argatroban, lepirudin, or danaparoid.
29169470	1	37	dep	anticoagulants	219:232	arg1	argatroban					234:243	argatroban	234:243	argatroban	234:243	BACKGROUND Life-threatening heparin-induced thrombocytopenia (HIT) is treated with the alternative nonheparin anticoagulants argatroban, lepirudin, or danaparoid.
29169470	5	38	theme	skin	875:878	arg1	lesions					880:886	skin lesions	875:886	skin lesions	875:886	Main outcome measures were the incidences of HIT-specific complications (thromboembolic venous/arterial events, amputations, recurrent/persistent thrombocytopenia, skin lesions) and bleedings.
29169470	5	38	theme	skin	875:878	arg1	events					815:820	thromboembolic venous/arterial events	784:820	thromboembolic venous/arterial events	784:820	Main outcome measures were the incidences of HIT-specific complications (thromboembolic venous/arterial events, amputations, recurrent/persistent thrombocytopenia, skin lesions) and bleedings.
29169470	7	39	theme	thromboembolic	1132:1145	arg1	amputation					1155:1164	amputation	1155:1164	amputation	1155:1164	The composite endpoint of HIT-specific complications (thromboembolic events, amputation, skin necrosis) occurred in 11.7% of patients treated with approved alternative anticoagulation and in 0.0% of fondaparinux-treated patients.
29169470	7	39	theme	thromboembolic	1132:1145	arg1	necrosis					1172:1179	skin necrosis	1167:1179	skin necrosis	1167:1179	The composite endpoint of HIT-specific complications (thromboembolic events, amputation, skin necrosis) occurred in 11.7% of patients treated with approved alternative anticoagulation and in 0.0% of fondaparinux-treated patients.
29169470	7	39	theme	thromboembolic	1132:1145	arg1	events					1147:1152	thromboembolic events	1132:1152	thromboembolic events	1132:1152	The composite endpoint of HIT-specific complications (thromboembolic events, amputation, skin necrosis) occurred in 11.7% of patients treated with approved alternative anticoagulation and in 0.0% of fondaparinux-treated patients.
29169470	11	40	theme	acute	1811:1815	arg1	HIT					1817:1819	suspected acute HIT	1801:1819	suspected acute HIT	1801:1819	CONCLUSIONS Fondaparinux is effective and safe in suspected acute HIT; no HIT-specific complications occurred in the fondaparinux-treated patients, even among those with a high clinical HIT probability.
29169470	7	41	theme	complications	1117:1129	arg1	endpoint					1092:1099	The composite endpoint	1078:1099	The composite endpoint of HIT-specific complications (thromboembolic events, amputation, skin necrosis)	1078:1180	The composite endpoint of HIT-specific complications (thromboembolic events, amputation, skin necrosis) occurred in 11.7% of patients treated with approved alternative anticoagulation and in 0.0% of fondaparinux-treated patients.
29169470	4	42	theme	clinical	582:589	arg1	HIT-risk					591:598	an at least intermediate clinical HIT-risk	557:598	an at least intermediate clinical HIT-risk (4Ts score ≥4 points)	557:620	METHODS In a national, multicenter registry study, hospitalized patients who were diagnosed with HIT, an at least intermediate clinical HIT-risk (4Ts score ≥4 points), and received treatment with ≥1 dose of the aforementioned anticoagulants were included.
29169470	4	42	theme	clinical	582:589	arg1	HIT					552:554	HIT	552:554	HIT	552:554	METHODS In a national, multicenter registry study, hospitalized patients who were diagnosed with HIT, an at least intermediate clinical HIT-risk (4Ts score ≥4 points), and received treatment with ≥1 dose of the aforementioned anticoagulants were included.
29169470	4	42	theme	clinical	582:589	arg1	points					614:619	4Ts score ≥4 points	601:619	4Ts score ≥4 points	601:619	METHODS In a national, multicenter registry study, hospitalized patients who were diagnosed with HIT, an at least intermediate clinical HIT-risk (4Ts score ≥4 points), and received treatment with ≥1 dose of the aforementioned anticoagulants were included.
29169470	4	43	theme	treatment	636:644	arg1	dose					654:657	treatment with ≥1 dose	636:657	treatment with ≥1 dose of the aforementioned anticoagulants	636:694	METHODS In a national, multicenter registry study, hospitalized patients who were diagnosed with HIT, an at least intermediate clinical HIT-risk (4Ts score ≥4 points), and received treatment with ≥1 dose of the aforementioned anticoagulants were included.
29169470	12	44	theme	United	2131:2136	arg1	States					2138:2143	the United States	2127:2143	the United States	2127:2143	Further data from randomized controlled trials are urgently needed because lepirudin was recalled from the market; danaparoid access has been limited and is not approved in the United States; and argatroban is contraindicated in patients with impaired liver function, and activated partial thromboplastin time confounding may interfere with monitoring.
29169470	11	45	theme	HIT-specific	1825:1836	arg1	complications					1838:1850	no HIT-specific complications	1822:1850	no HIT-specific complications	1822:1850	CONCLUSIONS Fondaparinux is effective and safe in suspected acute HIT; no HIT-specific complications occurred in the fondaparinux-treated patients, even among those with a high clinical HIT probability.
29169470	9	46	theme	Bleeding	1445:1452	arg1	complications					1454:1466	Bleeding complications	1445:1466	Bleeding complications	1445:1466	Bleeding complications occurred in alternatively anticoagulated patients and in fondaparinux-treated patients in 6.3% and 4.8%, respectively.
29169470	12	47	theme	controlled	1983:1992	arg1	trials					1994:1999	randomized controlled trials	1972:1999	randomized controlled trials	1972:1999	Further data from randomized controlled trials are urgently needed because lepirudin was recalled from the market; danaparoid access has been limited and is not approved in the United States; and argatroban is contraindicated in patients with impaired liver function, and activated partial thromboplastin time confounding may interfere with monitoring.
29169470	13	48	theme	Heparin-induced	2354:2368	arg1	Thrombocytopenia					2370:2385	Acute Heparin-induced Thrombocytopenia	2348:2385	Acute Heparin-induced Thrombocytopenia Type II	2348:2393	(Retrospective Registry of Patients With Acute Heparin-induced Thrombocytopenia Type II; NCT01304238).
29169470	7	49	theme	approved	1225:1232	arg1	anticoagulation					1246:1260	approved alternative anticoagulation	1225:1260	approved alternative anticoagulation	1225:1260	The composite endpoint of HIT-specific complications (thromboembolic events, amputation, skin necrosis) occurred in 11.7% of patients treated with approved alternative anticoagulation and in 0.0% of fondaparinux-treated patients.
29169470	2	50	used	used	320:323	arg2	fondaparinux					304:315	the pentasaccharide fondaparinux	284:315	the pentasaccharide fondaparinux	284:315	Frequently, the pentasaccharide fondaparinux is used off-label.
29169470	5	51	theme	recurrent/persistent	836:855	arg1	events					815:820	thromboembolic venous/arterial events	784:820	thromboembolic venous/arterial events	784:820	Main outcome measures were the incidences of HIT-specific complications (thromboembolic venous/arterial events, amputations, recurrent/persistent thrombocytopenia, skin lesions) and bleedings.
29169470	5	51	theme	recurrent/persistent	836:855	arg1	thrombocytopenia					857:872	recurrent/persistent thrombocytopenia	836:872	recurrent/persistent thrombocytopenia	836:872	Main outcome measures were the incidences of HIT-specific complications (thromboembolic venous/arterial events, amputations, recurrent/persistent thrombocytopenia, skin lesions) and bleedings.
29169470	12	52	from	trials	1994:1999	arg1	data					1962:1965	Further data	1954:1965	Further data from randomized controlled trials	1954:1999	Further data from randomized controlled trials are urgently needed because lepirudin was recalled from the market; danaparoid access has been limited and is not approved in the United States; and argatroban is contraindicated in patients with impaired liver function, and activated partial thromboplastin time confounding may interfere with monitoring.
29169470	11	53	from	effective	1779:1787	arg1	HIT					1817:1819	suspected acute HIT	1801:1819	suspected acute HIT	1801:1819	CONCLUSIONS Fondaparinux is effective and safe in suspected acute HIT; no HIT-specific complications occurred in the fondaparinux-treated patients, even among those with a high clinical HIT probability.
29169470	5	54	theme	Main	711:714	arg1	measures					724:731	Main outcome measures	711:731	Main outcome measures	711:731	Main outcome measures were the incidences of HIT-specific complications (thromboembolic venous/arterial events, amputations, recurrent/persistent thrombocytopenia, skin lesions) and bleedings.
29169470	5	54	theme	Main	711:714	arg1	incidences					742:751	the incidences	738:751	the incidences of HIT-specific complications (thromboembolic venous/arterial events, amputations, recurrent/persistent thrombocytopenia, skin lesions)	738:887	Main outcome measures were the incidences of HIT-specific complications (thromboembolic venous/arterial events, amputations, recurrent/persistent thrombocytopenia, skin lesions) and bleedings.
29169470	12	55	from	approved	2115:2122	arg1	States					2138:2143	the United States	2127:2143	the United States	2127:2143	Further data from randomized controlled trials are urgently needed because lepirudin was recalled from the market; danaparoid access has been limited and is not approved in the United States; and argatroban is contraindicated in patients with impaired liver function, and activated partial thromboplastin time confounding may interfere with monitoring.
29169470	12	56	theme	Further	1954:1960	arg1	data					1962:1965	Further data	1954:1965	Further data from randomized controlled trials	1954:1999	Further data from randomized controlled trials are urgently needed because lepirudin was recalled from the market; danaparoid access has been limited and is not approved in the United States; and argatroban is contraindicated in patients with impaired liver function, and activated partial thromboplastin time confounding may interfere with monitoring.
29169470	10	57	theme	Post	1587:1590	arg1	analysis					1596:1603	Post hoc analysis	1587:1603	Post hoc analysis of clinical and laboratory features	1587:1639	Post hoc analysis of clinical and laboratory features confirmed "true" HIT in at least 74 of 195 (38.0%) patients; 35 of 74 (47.3%) were treated with fondaparinux.
29169470	1	58	theme	alternative	196:206	arg1	anticoagulants					219:232	the alternative nonheparin anticoagulants argatroban, lepirudin, or danaparoid	192:269	the alternative nonheparin anticoagulants argatroban, lepirudin, or danaparoid	192:269	BACKGROUND Life-threatening heparin-induced thrombocytopenia (HIT) is treated with the alternative nonheparin anticoagulants argatroban, lepirudin, or danaparoid.
29169470	1	58	theme	alternative	196:206	arg1	lepirudin					246:254	lepirudin	246:254	lepirudin	246:254	BACKGROUND Life-threatening heparin-induced thrombocytopenia (HIT) is treated with the alternative nonheparin anticoagulants argatroban, lepirudin, or danaparoid.
29169470	1	58	theme	alternative	196:206	arg1	danaparoid					260:269	danaparoid	260:269	danaparoid	260:269	BACKGROUND Life-threatening heparin-induced thrombocytopenia (HIT) is treated with the alternative nonheparin anticoagulants argatroban, lepirudin, or danaparoid.
29169470	1	58	theme	alternative	196:206	arg1	argatroban					234:243	argatroban	234:243	argatroban	234:243	BACKGROUND Life-threatening heparin-induced thrombocytopenia (HIT) is treated with the alternative nonheparin anticoagulants argatroban, lepirudin, or danaparoid.
29169470	0	59	theme	Anticoagulants	42:55	arg1	Use					0:2	Use	0:2	Use of Fondaparinux Off-Label or Approved Anticoagulants for Management of Heparin-Induced Thrombocytopenia.	0:107	Use of Fondaparinux Off-Label or Approved Anticoagulants for Management of Heparin-Induced Thrombocytopenia.
29169470	10	60	theme	laboratory	1621:1630	arg1	features					1632:1639	clinical and laboratory features	1608:1639	clinical and laboratory features	1608:1639	Post hoc analysis of clinical and laboratory features confirmed "true" HIT in at least 74 of 195 (38.0%) patients; 35 of 74 (47.3%) were treated with fondaparinux.
29169470	10	61	theme	clinical	1608:1615	arg1	features					1632:1639	clinical and laboratory features	1608:1639	clinical and laboratory features	1608:1639	Post hoc analysis of clinical and laboratory features confirmed "true" HIT in at least 74 of 195 (38.0%) patients; 35 of 74 (47.3%) were treated with fondaparinux.
29169470	10	62	theme	true	1652:1655	arg1	HIT					1658:1660	"true" HIT	1651:1660	"true" HIT	1651:1660	Post hoc analysis of clinical and laboratory features confirmed "true" HIT in at least 74 of 195 (38.0%) patients; 35 of 74 (47.3%) were treated with fondaparinux.
29169470	5	63	theme	complications	769:781	arg1	bleedings					893:901	bleedings	893:901	bleedings	893:901	Main outcome measures were the incidences of HIT-specific complications (thromboembolic venous/arterial events, amputations, recurrent/persistent thrombocytopenia, skin lesions) and bleedings.
29169470	5	63	theme	complications	769:781	arg1	measures					724:731	Main outcome measures	711:731	Main outcome measures	711:731	Main outcome measures were the incidences of HIT-specific complications (thromboembolic venous/arterial events, amputations, recurrent/persistent thrombocytopenia, skin lesions) and bleedings.
29169470	5	63	theme	complications	769:781	arg1	incidences					742:751	the incidences	738:751	the incidences of HIT-specific complications (thromboembolic venous/arterial events, amputations, recurrent/persistent thrombocytopenia, skin lesions)	738:887	Main outcome measures were the incidences of HIT-specific complications (thromboembolic venous/arterial events, amputations, recurrent/persistent thrombocytopenia, skin lesions) and bleedings.
29169470	9	64	theme	anticoagulated	1494:1507	arg1	patients					1509:1516	alternatively anticoagulated patients	1480:1516	alternatively anticoagulated patients	1480:1516	Bleeding complications occurred in alternatively anticoagulated patients and in fondaparinux-treated patients in 6.3% and 4.8%, respectively.
29169470	4	65	theme	anticoagulants	681:694	arg1	dose					654:657	treatment with ≥1 dose	636:657	treatment with ≥1 dose of the aforementioned anticoagulants	636:694	METHODS In a national, multicenter registry study, hospitalized patients who were diagnosed with HIT, an at least intermediate clinical HIT-risk (4Ts score ≥4 points), and received treatment with ≥1 dose of the aforementioned anticoagulants were included.
29169470	9	66	theme	fondaparinux-treated	1525:1544	arg1	patients					1546:1553	fondaparinux-treated patients	1525:1553	fondaparinux-treated patients in 6.3% and 4.8%, respectively	1525:1584	Bleeding complications occurred in alternatively anticoagulated patients and in fondaparinux-treated patients in 6.3% and 4.8%, respectively.
29169470	4	67	theme	registry	490:497	arg1	study					499:503	a national, multicenter registry study	466:503	study	499:503	METHODS In a national, multicenter registry study, hospitalized patients who were diagnosed with HIT, an at least intermediate clinical HIT-risk (4Ts score ≥4 points), and received treatment with ≥1 dose of the aforementioned anticoagulants were included.
29169470	4	67	theme	registry	490:497	arg1	patients					519:526	hospitalized patients	506:526	hospitalized patients who were diagnosed with HIT, an at least intermediate clinical HIT-risk (4Ts score ≥4 points), and received treatment with ≥1 dose of the aforementioned anticoagulants	506:694	METHODS In a national, multicenter registry study, hospitalized patients who were diagnosed with HIT, an at least intermediate clinical HIT-risk (4Ts score ≥4 points), and received treatment with ≥1 dose of the aforementioned anticoagulants were included.
29169470	12	68	theme	impaired	2197:2204	arg1	function					2212:2219	impaired liver function	2197:2219	impaired liver function	2197:2219	Further data from randomized controlled trials are urgently needed because lepirudin was recalled from the market; danaparoid access has been limited and is not approved in the United States; and argatroban is contraindicated in patients with impaired liver function, and activated partial thromboplastin time confounding may interfere with monitoring.
29169470	11	69	theme	clinical	1928:1935	arg1	probability					1941:1951	a high clinical HIT probability	1921:1951	a high clinical HIT probability	1921:1951	CONCLUSIONS Fondaparinux is effective and safe in suspected acute HIT; no HIT-specific complications occurred in the fondaparinux-treated patients, even among those with a high clinical HIT probability.
29169470	12	70	theme	danaparoid	2069:2078	arg1	access					2080:2085	danaparoid access	2069:2085	danaparoid access	2069:2085	Further data from randomized controlled trials are urgently needed because lepirudin was recalled from the market; danaparoid access has been limited and is not approved in the United States; and argatroban is contraindicated in patients with impaired liver function, and activated partial thromboplastin time confounding may interfere with monitoring.
29169470	12	71	theme	time	2259:2262	arg1	confounding					2264:2274	activated partial thromboplastin time confounding	2226:2274	activated partial thromboplastin time confounding	2226:2274	Further data from randomized controlled trials are urgently needed because lepirudin was recalled from the market; danaparoid access has been limited and is not approved in the United States; and argatroban is contraindicated in patients with impaired liver function, and activated partial thromboplastin time confounding may interfere with monitoring.
29169470	12	72	with	patients	2183:2190	arg1	function					2212:2219	impaired liver function	2197:2219	impaired liver function	2197:2219	Further data from randomized controlled trials are urgently needed because lepirudin was recalled from the market; danaparoid access has been limited and is not approved in the United States; and argatroban is contraindicated in patients with impaired liver function, and activated partial thromboplastin time confounding may interfere with monitoring.
29169470	0	73	theme	Thrombocytopenia	91:106	arg1	Management					61:70	Management	61:70	Management of Heparin-Induced Thrombocytopenia	61:106	Use of Fondaparinux Off-Label or Approved Anticoagulants for Management of Heparin-Induced Thrombocytopenia.
29169470	5	74	theme	thromboembolic	784:797	arg1	events					815:820	thromboembolic venous/arterial events	784:820	thromboembolic venous/arterial events	784:820	Main outcome measures were the incidences of HIT-specific complications (thromboembolic venous/arterial events, amputations, recurrent/persistent thrombocytopenia, skin lesions) and bleedings.
29169470	5	74	theme	thromboembolic	784:797	arg1	lesions					880:886	skin lesions	875:886	skin lesions	875:886	Main outcome measures were the incidences of HIT-specific complications (thromboembolic venous/arterial events, amputations, recurrent/persistent thrombocytopenia, skin lesions) and bleedings.
29169470	5	74	theme	thromboembolic	784:797	arg1	amputations					823:833	amputations	823:833	amputations	823:833	Main outcome measures were the incidences of HIT-specific complications (thromboembolic venous/arterial events, amputations, recurrent/persistent thrombocytopenia, skin lesions) and bleedings.
29169470	5	74	theme	thromboembolic	784:797	arg1	thrombocytopenia					857:872	recurrent/persistent thrombocytopenia	836:872	recurrent/persistent thrombocytopenia	836:872	Main outcome measures were the incidences of HIT-specific complications (thromboembolic venous/arterial events, amputations, recurrent/persistent thrombocytopenia, skin lesions) and bleedings.
29169470	3	75	theme	different	412:420	arg1	anticoagulants					422:435	the different anticoagulants	408:435	the different anticoagulants	408:435	OBJECTIVES The authors sought to investigate the safety and efficacy of the different anticoagulants for treating HIT.
29169470	8	76	theme	in-hospital	1322:1332	arg1	rates					1344:1348	The all-cause in-hospital mortality rates	1308:1348	The all-cause in-hospital mortality rates	1308:1348	The all-cause in-hospital mortality rates were 14.4% during approved alternative anticoagulation and 0.0% during fondaparinux treatment.
29169470	8	76	theme	in-hospital	1322:1332	arg1	%					1359:1359	14.4%	1355:1359	14.4% during approved alternative anticoagulation	1355:1403	The all-cause in-hospital mortality rates were 14.4% during approved alternative anticoagulation and 0.0% during fondaparinux treatment.
29169470	7	77	theme	skin	1167:1170	arg1	necrosis					1172:1179	skin necrosis	1167:1179	skin necrosis	1167:1179	The composite endpoint of HIT-specific complications (thromboembolic events, amputation, skin necrosis) occurred in 11.7% of patients treated with approved alternative anticoagulation and in 0.0% of fondaparinux-treated patients.
29169470	7	77	theme	skin	1167:1170	arg1	events					1147:1152	thromboembolic events	1132:1152	thromboembolic events	1132:1152	The composite endpoint of HIT-specific complications (thromboembolic events, amputation, skin necrosis) occurred in 11.7% of patients treated with approved alternative anticoagulation and in 0.0% of fondaparinux-treated patients.
29169470	12	78	theme	partial	2236:2242	arg1	confounding					2264:2274	activated partial thromboplastin time confounding	2226:2274	activated partial thromboplastin time confounding	2226:2274	Further data from randomized controlled trials are urgently needed because lepirudin was recalled from the market; danaparoid access has been limited and is not approved in the United States; and argatroban is contraindicated in patients with impaired liver function, and activated partial thromboplastin time confounding may interfere with monitoring.
29169470	7	79	dep	complications	1117:1129	arg1	amputation					1155:1164	amputation	1155:1164	amputation	1155:1164	The composite endpoint of HIT-specific complications (thromboembolic events, amputation, skin necrosis) occurred in 11.7% of patients treated with approved alternative anticoagulation and in 0.0% of fondaparinux-treated patients.
29169470	7	79	dep	complications	1117:1129	arg1	necrosis					1172:1179	skin necrosis	1167:1179	skin necrosis	1167:1179	The composite endpoint of HIT-specific complications (thromboembolic events, amputation, skin necrosis) occurred in 11.7% of patients treated with approved alternative anticoagulation and in 0.0% of fondaparinux-treated patients.
29169470	7	79	dep	complications	1117:1129	arg1	events					1147:1152	thromboembolic events	1132:1152	thromboembolic events	1132:1152	The composite endpoint of HIT-specific complications (thromboembolic events, amputation, skin necrosis) occurred in 11.7% of patients treated with approved alternative anticoagulation and in 0.0% of fondaparinux-treated patients.
29169470	12	80	theme	liver	2206:2210	arg1	function					2212:2219	impaired liver function	2197:2219	impaired liver function	2197:2219	Further data from randomized controlled trials are urgently needed because lepirudin was recalled from the market; danaparoid access has been limited and is not approved in the United States; and argatroban is contraindicated in patients with impaired liver function, and activated partial thromboplastin time confounding may interfere with monitoring.
29169470	11	81	theme	fondaparinux-treated	1868:1887	arg1	patients					1889:1896	the fondaparinux-treated patients	1864:1896	the fondaparinux-treated patients	1864:1896	CONCLUSIONS Fondaparinux is effective and safe in suspected acute HIT; no HIT-specific complications occurred in the fondaparinux-treated patients, even among those with a high clinical HIT probability.
29169470	7	82	theme	patients	1298:1305	arg1	%					1272:1272	0.0%	1269:1272	0.0% of fondaparinux-treated patients	1269:1305	The composite endpoint of HIT-specific complications (thromboembolic events, amputation, skin necrosis) occurred in 11.7% of patients treated with approved alternative anticoagulation and in 0.0% of fondaparinux-treated patients.
29169470	7	82	theme	patients	1298:1305	arg1	patients					1298:1305	fondaparinux-treated patients	1277:1305	fondaparinux-treated patients	1277:1305	The composite endpoint of HIT-specific complications (thromboembolic events, amputation, skin necrosis) occurred in 11.7% of patients treated with approved alternative anticoagulation and in 0.0% of fondaparinux-treated patients.
29169470	4	83	theme	4Ts	601:603	arg1	HIT-risk					591:598	an at least intermediate clinical HIT-risk	557:598	an at least intermediate clinical HIT-risk (4Ts score ≥4 points)	557:620	METHODS In a national, multicenter registry study, hospitalized patients who were diagnosed with HIT, an at least intermediate clinical HIT-risk (4Ts score ≥4 points), and received treatment with ≥1 dose of the aforementioned anticoagulants were included.
29169470	4	83	theme	4Ts	601:603	arg1	points					614:619	4Ts score ≥4 points	601:619	4Ts score ≥4 points	601:619	METHODS In a national, multicenter registry study, hospitalized patients who were diagnosed with HIT, an at least intermediate clinical HIT-risk (4Ts score ≥4 points), and received treatment with ≥1 dose of the aforementioned anticoagulants were included.
29169470	10	84	theme	"	1656:1656	arg1	HIT					1658:1660	"true" HIT	1651:1660	"true" HIT	1651:1660	Post hoc analysis of clinical and laboratory features confirmed "true" HIT in at least 74 of 195 (38.0%) patients; 35 of 74 (47.3%) were treated with fondaparinux.
29169470	0	85	theme	Off-Label	20:28	arg1	Anticoagulants					42:55	Fondaparinux Off-Label or Approved Anticoagulants	7:55	Fondaparinux Off-Label or Approved Anticoagulants	7:55	Use of Fondaparinux Off-Label or Approved Anticoagulants for Management of Heparin-Induced Thrombocytopenia.
29169470	1	86	theme	Life-threatening	120:135	arg1	HIT					171:173	HIT	171:173	HIT	171:173	BACKGROUND Life-threatening heparin-induced thrombocytopenia (HIT) is treated with the alternative nonheparin anticoagulants argatroban, lepirudin, or danaparoid.
29169470	1	86	theme	Life-threatening	120:135	arg1	thrombocytopenia					153:168	BACKGROUND Life-threatening heparin-induced thrombocytopenia	109:168	BACKGROUND Life-threatening heparin-induced thrombocytopenia (HIT)	109:174	BACKGROUND Life-threatening heparin-induced thrombocytopenia (HIT) is treated with the alternative nonheparin anticoagulants argatroban, lepirudin, or danaparoid.
29169470	13	87	theme	Retrospective	2308:2320	arg1	Registry					2322:2329	Retrospective Registry	2308:2329	Retrospective Registry of Patients With Acute Heparin-induced Thrombocytopenia Type II; NCT01304238	2308:2406	(Retrospective Registry of Patients With Acute Heparin-induced Thrombocytopenia Type II; NCT01304238).
29169470	0	88	theme	Approved	33:40	arg1	Anticoagulants					42:55	Fondaparinux Off-Label or Approved Anticoagulants	7:55	Fondaparinux Off-Label or Approved Anticoagulants	7:55	Use of Fondaparinux Off-Label or Approved Anticoagulants for Management of Heparin-Induced Thrombocytopenia.
29169470	13	89	theme	Thrombocytopenia	2370:2385	arg1	Type					2387:2390	Acute Heparin-induced Thrombocytopenia Type II	2348:2393	Acute Heparin-induced Thrombocytopenia Type II	2348:2393	(Retrospective Registry of Patients With Acute Heparin-induced Thrombocytopenia Type II; NCT01304238).
29169470	12	90	from	States	2138:2143	arg1	approved					2115:2122	approved	2115:2122	approved	2115:2122	Further data from randomized controlled trials are urgently needed because lepirudin was recalled from the market; danaparoid access has been limited and is not approved in the United States; and argatroban is contraindicated in patients with impaired liver function, and activated partial thromboplastin time confounding may interfere with monitoring.
29169470	8	91	theme	alternative	1377:1387	arg1	anticoagulation					1389:1403	approved alternative anticoagulation	1368:1403	approved alternative anticoagulation	1368:1403	The all-cause in-hospital mortality rates were 14.4% during approved alternative anticoagulation and 0.0% during fondaparinux treatment.
29169470	7	92	theme	HIT-specific	1104:1115	arg1	complications					1117:1129	HIT-specific complications	1104:1129	HIT-specific complications (thromboembolic events, amputation, skin necrosis)	1104:1180	The composite endpoint of HIT-specific complications (thromboembolic events, amputation, skin necrosis) occurred in 11.7% of patients treated with approved alternative anticoagulation and in 0.0% of fondaparinux-treated patients.
29169470	6	93	theme	patients	919:926	arg1	RESULTS					904:910	RESULTS	904:910	RESULTS Of 195 patients, 46 (23.6%), 4 (2.1%), 61 (31.3%), and 84 (43.1%)	904:976	RESULTS Of 195 patients, 46 (23.6%), 4 (2.1%), 61 (31.3%), and 84 (43.1%) had been treated first-line with argatroban, lepirudin, danaparoid, and fondaparinux, respectively.
29169470	13	94	theme	Patients	2334:2341	arg1	Registry					2322:2329	Retrospective Registry	2308:2329	Retrospective Registry of Patients With Acute Heparin-induced Thrombocytopenia Type II; NCT01304238	2308:2406	(Retrospective Registry of Patients With Acute Heparin-induced Thrombocytopenia Type II; NCT01304238).
29169470	7	95	theme	alternative	1234:1244	arg1	anticoagulation					1246:1260	approved alternative anticoagulation	1225:1260	approved alternative anticoagulation	1225:1260	The composite endpoint of HIT-specific complications (thromboembolic events, amputation, skin necrosis) occurred in 11.7% of patients treated with approved alternative anticoagulation and in 0.0% of fondaparinux-treated patients.
29169470	4	96	theme	with ≥1	646:652	arg1	dose					654:657	treatment with ≥1 dose	636:657	treatment with ≥1 dose of the aforementioned anticoagulants	636:694	METHODS In a national, multicenter registry study, hospitalized patients who were diagnosed with HIT, an at least intermediate clinical HIT-risk (4Ts score ≥4 points), and received treatment with ≥1 dose of the aforementioned anticoagulants were included.
29169470	11	97	theme	suspected	1801:1809	arg1	HIT					1817:1819	suspected acute HIT	1801:1819	suspected acute HIT	1801:1819	CONCLUSIONS Fondaparinux is effective and safe in suspected acute HIT; no HIT-specific complications occurred in the fondaparinux-treated patients, even among those with a high clinical HIT probability.
29169470	13	98	theme	Acute	2348:2352	arg1	Thrombocytopenia					2370:2385	Acute Heparin-induced Thrombocytopenia	2348:2385	Acute Heparin-induced Thrombocytopenia Type II	2348:2393	(Retrospective Registry of Patients With Acute Heparin-induced Thrombocytopenia Type II; NCT01304238).
29169470	8	99	theme	fondaparinux	1421:1432	arg1	treatment					1434:1442	fondaparinux treatment	1421:1442	fondaparinux treatment	1421:1442	The all-cause in-hospital mortality rates were 14.4% during approved alternative anticoagulation and 0.0% during fondaparinux treatment.
29166575	14	0	theme	test	2187:2190	arg1	hours					2174:2178	all hours	2170:2178	all hours of the test	2170:2190	With the same dosage, CGE also reduced the carrageenan-induced paw edema at all hours of the test, confirming its anti-inflammatory effect.
29166575	9	1	from	levels	1453:1458	arg1	CGE					1421:1423	CGE depend	1421:1430	CGE depend of reduction in PGE2 levels	1421:1458	We investigated if the analgesic and anti-inflammatory effects of CGE depend of reduction in PGE2 levels, were performed the carrageenan or PGE2-induced hyperalgesia tests.
29166575	7	2	theme	acetic	1120:1125	arg1	test					1149:1152	acetic acid-induced writhing test	1120:1152	acetic acid-induced writhing test	1120:1152	CGE was analyzed via the Irwin test, acetic acid-induced writhing test, formalin-induced pain test, and carrageenan-induced paw edema test.
29166575	10	3	theme	anacardic	1596:1604	arg1	acids					1606:1610	anacardic acids	1596:1610	anacardic acids	1596:1610	RESULTS The chemical characterization of CGE showed the presence of anacardic acids as the predominant phytoconstituents.
29166575	1	4	from	diabetes	311:318	arg1	treatment					265:273	the treatment	261:273	the treatment of inflammatory conditions	261:300	ETHNOPHARMACOLOGICAL RELEVANCE The cashew gum (Anacardium occidentale L.) is used in traditional Brazilian medicine in the treatment of inflammatory conditions, asthma, diabetes, and gastrointestinal disturbances.
29166575	3	5	dep	precipitation	673:685	arg1	the					669:671	the	669:671	the	669:671	MATERIALS AND METHODS The CGE was obtained after the precipitation and removal of polysaccharides through the use of acetone.
29166575	5	6	theme	mass	919:922	arg1	spectrometry					924:935	liquid chromatography mass spectrometry	897:935	liquid chromatography mass spectrometry (LC-MS)	897:943	The chemical characterization of CGE was performed by liquid chromatography mass spectrometry (LC-MS) and tandem mass spectrometry (MS/MS) analyses.
29166575	5	6	theme	mass	919:922	arg1	LC-MS					938:942	LC-MS	938:942	LC-MS	938:942	The chemical characterization of CGE was performed by liquid chromatography mass spectrometry (LC-MS) and tandem mass spectrometry (MS/MS) analyses.
29166575	1	7	from	asthma	303:308	arg1	treatment					265:273	the treatment	261:273	the treatment of inflammatory conditions	261:300	ETHNOPHARMACOLOGICAL RELEVANCE The cashew gum (Anacardium occidentale L.) is used in traditional Brazilian medicine in the treatment of inflammatory conditions, asthma, diabetes, and gastrointestinal disturbances.
29166575	2	8	attach	presence	566:573	arg1	CGE					614:616	CGE	614:616	CGE	614:616	AIM OF THE STUDY In the present study, we aimed at forming a chemical characterization and investigation of the antinociceptive and anti-inflammatory activities of the aqueous extract of cashew gum without the presence of polysaccharides in its composition (CGE).
29166575	2	8	attach	presence	566:573	arg2	polysaccharides					578:592	polysaccharides	578:592	polysaccharides	578:592	AIM OF THE STUDY In the present study, we aimed at forming a chemical characterization and investigation of the antinociceptive and anti-inflammatory activities of the aqueous extract of cashew gum without the presence of polysaccharides in its composition (CGE).
29166575	2	8	attach	presence	566:573	arg1	composition					601:611	its composition	597:611	its composition (CGE)	597:617	AIM OF THE STUDY In the present study, we aimed at forming a chemical characterization and investigation of the antinociceptive and anti-inflammatory activities of the aqueous extract of cashew gum without the presence of polysaccharides in its composition (CGE).
29166575	11	9	dep	CGE	1669:1671	arg1	75					1674:1675	75	1674:1675	75	1674:1675	The treatment with CGE (75, 150, and 300mg/kg, p.o.) inhibited the number of writhing in a dose-dependent manner.
29166575	11	9	dep	CGE	1669:1671	arg1	p.o.					1697:1700	p.o.	1697:1700	p.o.	1697:1700	The treatment with CGE (75, 150, and 300mg/kg, p.o.) inhibited the number of writhing in a dose-dependent manner.
29166575	11	9	dep	CGE	1669:1671	arg1	150					1678:1680	150	1678:1680	150	1678:1680	The treatment with CGE (75, 150, and 300mg/kg, p.o.) inhibited the number of writhing in a dose-dependent manner.
29166575	11	9	dep	CGE	1669:1671	arg1	300mg/kg					1687:1694	300mg/kg	1687:1694	300mg/kg	1687:1694	The treatment with CGE (75, 150, and 300mg/kg, p.o.) inhibited the number of writhing in a dose-dependent manner.
29166575	10	10	theme	predominant	1619:1629	arg1	phytoconstituents					1631:1647	the predominant phytoconstituents	1615:1647	the predominant phytoconstituents	1615:1647	RESULTS The chemical characterization of CGE showed the presence of anacardic acids as the predominant phytoconstituents.
29166575	10	10	theme	predominant	1619:1629	arg1	presence					1584:1591	the presence	1580:1591	the presence of anacardic acids	1580:1610	RESULTS The chemical characterization of CGE showed the presence of anacardic acids as the predominant phytoconstituents.
29166575	13	11	theme	pain	1951:1954	arg1	test					1956:1959	the formalin-induced pain test	1930:1959	the formalin-induced pain test	1930:1959	In the formalin-induced pain test, CGE (150mg/kg, p.o.) produced an antinociceptive effect only in the first phase of the test, suggesting anti-inflammatory activity.
29166575	16	12	theme	PGE2	2538:2541	arg1	levels					2543:2548	the PGE2 levels	2534:2548	the PGE2 levels	2534:2548	However, this dose of CGE was not able to reduce the hyperalgesia induced by PGE2, suggesting that the anti-inflammatory effect of this extract depends on the reduction in the PGE2 levels.
29166575	19	13	theme	effects	2850:2856	arg1	part					2800:2803	part	2800:2803	part of its antinociceptive and anti-inflammatory effects	2800:2856	These findings support the use of cashew gum in popular medicine and demonstrate that part of its antinociceptive and anti-inflammatory effects should also be attributed to the presence of anacardic acids in its composition, independent of the presence of polysaccharides.
29166575	3	14	theme	acetone	737:743	arg1	use					730:732	the use	726:732	the use of acetone	726:743	MATERIALS AND METHODS The CGE was obtained after the precipitation and removal of polysaccharides through the use of acetone.
29166575	15	15	theme	hyperalgesia	2343:2354	arg1	test					2356:2359	the carrageenan-induced hyperalgesia test	2319:2359	the carrageenan-induced hyperalgesia test	2319:2359	Furthermore, CGE (150mg/kg, p.o.) presented an antihyperalgic effect at all hours of the carrageenan-induced hyperalgesia test.
29166575	19	16	theme	cashew	2748:2753	arg1	gum					2755:2757	cashew gum	2748:2757	cashew gum	2748:2757	These findings support the use of cashew gum in popular medicine and demonstrate that part of its antinociceptive and anti-inflammatory effects should also be attributed to the presence of anacardic acids in its composition, independent of the presence of polysaccharides.
29166575	2	17	theme	gum	550:552	arg1	extract					532:538	the aqueous extract	520:538	the aqueous extract of cashew gum	520:552	AIM OF THE STUDY In the present study, we aimed at forming a chemical characterization and investigation of the antinociceptive and anti-inflammatory activities of the aqueous extract of cashew gum without the presence of polysaccharides in its composition (CGE).
29166575	9	18	dep	CGE	1421:1423	arg1	depend					1425:1430	depend	1425:1430	depend	1425:1430	We investigated if the analgesic and anti-inflammatory effects of CGE depend of reduction in PGE2 levels, were performed the carrageenan or PGE2-induced hyperalgesia tests.
29166575	1	19	used	used	219:222	arg2	RELEVANCE					163:171	ETHNOPHARMACOLOGICAL RELEVANCE The cashew gum (Anacardium occidentale L.)	142:214	ETHNOPHARMACOLOGICAL RELEVANCE The cashew gum (Anacardium occidentale L.)	142:214	ETHNOPHARMACOLOGICAL RELEVANCE The cashew gum (Anacardium occidentale L.) is used in traditional Brazilian medicine in the treatment of inflammatory conditions, asthma, diabetes, and gastrointestinal disturbances.
29166575	7	20	theme	formalin-induced	1155:1170	arg1	test					1177:1180	formalin-induced pain test	1155:1180	formalin-induced pain test	1155:1180	CGE was analyzed via the Irwin test, acetic acid-induced writhing test, formalin-induced pain test, and carrageenan-induced paw edema test.
29166575	5	21	theme	chemical	847:854	arg1	characterization					856:871	The chemical characterization	843:871	The chemical characterization of CGE	843:878	The chemical characterization of CGE was performed by liquid chromatography mass spectrometry (LC-MS) and tandem mass spectrometry (MS/MS) analyses.
29166575	8	22	theme	motor	1227:1231	arg1	activity					1233:1240	The motor activity	1223:1240	The motor activity	1223:1240	The motor activity or probable sedation was verified through the chimney, open-field, and sodium pentobarbital-induced sleep tests.
29166575	19	23	theme	antinociceptive	2812:2826	arg1	effects					2850:2856	its antinociceptive and anti-inflammatory effects	2808:2856	its antinociceptive and anti-inflammatory effects	2808:2856	These findings support the use of cashew gum in popular medicine and demonstrate that part of its antinociceptive and anti-inflammatory effects should also be attributed to the presence of anacardic acids in its composition, independent of the presence of polysaccharides.
29166575	1	24	from	medicine	249:256	arg1	treatment					265:273	the treatment	261:273	the treatment of inflammatory conditions	261:300	ETHNOPHARMACOLOGICAL RELEVANCE The cashew gum (Anacardium occidentale L.) is used in traditional Brazilian medicine in the treatment of inflammatory conditions, asthma, diabetes, and gastrointestinal disturbances.
29166575	9	25	theme	hyperalgesia	1508:1519	arg1	tests					1521:1525	the carrageenan or PGE2-induced hyperalgesia tests	1476:1525	the carrageenan or PGE2-induced hyperalgesia tests	1476:1525	We investigated if the analgesic and anti-inflammatory effects of CGE depend of reduction in PGE2 levels, were performed the carrageenan or PGE2-induced hyperalgesia tests.
29166575	7	26	theme	writhing	1140:1147	arg1	test					1149:1152	acetic acid-induced writhing test	1120:1152	acetic acid-induced writhing test	1120:1152	CGE was analyzed via the Irwin test, acetic acid-induced writhing test, formalin-induced pain test, and carrageenan-induced paw edema test.
29166575	18	27	from	reduction	2684:2692	arg1	levels					2706:2711	the PGE2 levels	2697:2711	the PGE2 levels	2697:2711	The action mechanisms of CGE suggest the reduction in the PGE2 levels.
29166575	11	28	theme	dose-dependent	1741:1754	arg1	manner					1756:1761	a dose-dependent manner	1739:1761	a dose-dependent manner	1739:1761	The treatment with CGE (75, 150, and 300mg/kg, p.o.) inhibited the number of writhing in a dose-dependent manner.
29166575	17	29	theme	anacardic	2566:2574	arg1	phytoconstituents					2602:2618	the predominant phytoconstituents	2586:2618	the predominant phytoconstituents identified in the CGE	2586:2640	CONCLUSION The anacardic acids are the predominant phytoconstituents identified in the CGE.
29166575	17	29	theme	anacardic	2566:2574	arg1	acids					2576:2580	The anacardic acids	2562:2580	The anacardic acids	2562:2580	CONCLUSION The anacardic acids are the predominant phytoconstituents identified in the CGE.
29166575	10	30	dep	RESULTS	1528:1534	arg1	showed					1573:1578	showed	1573:1578	showed the presence of anacardic acids as the predominant phytoconstituents	1573:1647	RESULTS The chemical characterization of CGE showed the presence of anacardic acids as the predominant phytoconstituents.
29166575	1	31	theme	gastrointestinal	325:340	arg1	disturbances					342:353	gastrointestinal disturbances	325:353	gastrointestinal disturbances	325:353	ETHNOPHARMACOLOGICAL RELEVANCE The cashew gum (Anacardium occidentale L.) is used in traditional Brazilian medicine in the treatment of inflammatory conditions, asthma, diabetes, and gastrointestinal disturbances.
29166575	2	32	theme	anti-inflammatory	488:504	arg1	activities					506:515	the antinociceptive and anti-inflammatory activities	464:515	the antinociceptive and anti-inflammatory activities of the aqueous extract of cashew gum	464:552	AIM OF THE STUDY In the present study, we aimed at forming a chemical characterization and investigation of the antinociceptive and anti-inflammatory activities of the aqueous extract of cashew gum without the presence of polysaccharides in its composition (CGE).
29166575	19	33	theme	anacardic	2903:2911	arg1	acids					2913:2917	anacardic acids	2903:2917	anacardic acids	2903:2917	These findings support the use of cashew gum in popular medicine and demonstrate that part of its antinociceptive and anti-inflammatory effects should also be attributed to the presence of anacardic acids in its composition, independent of the presence of polysaccharides.
29166575	8	34	theme	sleep	1342:1346	arg1	tests					1348:1352	the chimney, open-field, and sodium pentobarbital-induced sleep tests	1284:1352	the chimney, open-field, and sodium pentobarbital-induced sleep tests	1284:1352	The motor activity or probable sedation was verified through the chimney, open-field, and sodium pentobarbital-induced sleep tests.
29166575	0	35	theme	cashew	94:99	arg1	L					137:137	Anacardium occidentale L.	114:138	Anacardium occidentale L.	114:138	Chemical characterization and pharmacological assessment of polysaccharide free, standardized cashew gum extract (Anacardium occidentale L.).
29166575	0	35	theme	cashew	94:99	arg1	polysaccharide					60:73	polysaccharide free	60:78	polysaccharide free	60:78	Chemical characterization and pharmacological assessment of polysaccharide free, standardized cashew gum extract (Anacardium occidentale L.).
29166575	0	35	theme	cashew	94:99	arg1	extract					105:111	standardized cashew gum extract	81:111	standardized cashew gum extract (Anacardium occidentale L.)	81:139	Chemical characterization and pharmacological assessment of polysaccharide free, standardized cashew gum extract (Anacardium occidentale L.).
29166575	13	36	theme	test	2049:2052	arg1	phase					2036:2040	the first phase	2026:2040	the first phase of the test	2026:2052	In the formalin-induced pain test, CGE (150mg/kg, p.o.) produced an antinociceptive effect only in the first phase of the test, suggesting anti-inflammatory activity.
29166575	12	37	theme	pentobarbital-induced	1898:1918	arg1	sleep					1920:1924	either the open-field or sodium pentobarbital-induced sleep	1866:1924	sleep	1920:1924	With an intermediate dose, CGE did not cause motor impairment with the chimney test or alterations in either the open-field or sodium pentobarbital-induced sleep.
29166575	1	38	theme	cashew	177:182	arg1	gum					184:186	The cashew gum	173:186	ETHNOPHARMACOLOGICAL RELEVANCE The cashew gum (Anacardium occidentale L.)	142:214	ETHNOPHARMACOLOGICAL RELEVANCE The cashew gum (Anacardium occidentale L.) is used in traditional Brazilian medicine in the treatment of inflammatory conditions, asthma, diabetes, and gastrointestinal disturbances.
29166575	1	38	theme	cashew	177:182	arg1	L.					212:213	L.	212:213	L.	212:213	ETHNOPHARMACOLOGICAL RELEVANCE The cashew gum (Anacardium occidentale L.) is used in traditional Brazilian medicine in the treatment of inflammatory conditions, asthma, diabetes, and gastrointestinal disturbances.
29166575	18	39	theme	CGE	2668:2670	arg1	mechanisms					2654:2663	The action mechanisms	2643:2663	The action mechanisms of CGE	2643:2670	The action mechanisms of CGE suggest the reduction in the PGE2 levels.
29166575	7	40	theme	edema	1211:1215	arg1	test					1217:1220	carrageenan-induced paw edema test	1187:1220	carrageenan-induced paw edema test	1187:1220	CGE was analyzed via the Irwin test, acetic acid-induced writhing test, formalin-induced pain test, and carrageenan-induced paw edema test.
29166575	2	41	theme	aqueous	524:530	arg1	extract					532:538	the aqueous extract	520:538	the aqueous extract of cashew gum	520:552	AIM OF THE STUDY In the present study, we aimed at forming a chemical characterization and investigation of the antinociceptive and anti-inflammatory activities of the aqueous extract of cashew gum without the presence of polysaccharides in its composition (CGE).
29166575	17	42	dep	CONCLUSION	2551:2560	arg1	phytoconstituents					2602:2618	the predominant phytoconstituents	2586:2618	the predominant phytoconstituents identified in the CGE	2586:2640	CONCLUSION The anacardic acids are the predominant phytoconstituents identified in the CGE.
29166575	17	42	dep	CONCLUSION	2551:2560	arg1	acids					2576:2580	The anacardic acids	2562:2580	The anacardic acids	2562:2580	CONCLUSION The anacardic acids are the predominant phytoconstituents identified in the CGE.
29166575	0	43	theme	Anacardium	114:123	arg1	L					137:137	Anacardium occidentale L.	114:138	Anacardium occidentale L.	114:138	Chemical characterization and pharmacological assessment of polysaccharide free, standardized cashew gum extract (Anacardium occidentale L.).
29166575	0	43	theme	Anacardium	114:123	arg1	extract					105:111	standardized cashew gum extract	81:111	standardized cashew gum extract (Anacardium occidentale L.)	81:139	Chemical characterization and pharmacological assessment of polysaccharide free, standardized cashew gum extract (Anacardium occidentale L.).
29166575	13	44	theme	first	2030:2034	arg1	phase					2036:2040	the first phase	2026:2040	the first phase of the test	2026:2052	In the formalin-induced pain test, CGE (150mg/kg, p.o.) produced an antinociceptive effect only in the first phase of the test, suggesting anti-inflammatory activity.
29166575	2	45	theme	present	380:386	arg1	study					388:392	the present study	376:392	the present study	376:392	AIM OF THE STUDY In the present study, we aimed at forming a chemical characterization and investigation of the antinociceptive and anti-inflammatory activities of the aqueous extract of cashew gum without the presence of polysaccharides in its composition (CGE).
29166575	0	46	theme	Chemical	0:7	arg1	characterization					9:24	Chemical characterization	0:24	Chemical characterization	0:24	Chemical characterization and pharmacological assessment of polysaccharide free, standardized cashew gum extract (Anacardium occidentale L.).
29166575	1	47	theme	inflammatory	278:289	arg1	conditions					291:300	inflammatory conditions	278:300	inflammatory conditions	278:300	ETHNOPHARMACOLOGICAL RELEVANCE The cashew gum (Anacardium occidentale L.) is used in traditional Brazilian medicine in the treatment of inflammatory conditions, asthma, diabetes, and gastrointestinal disturbances.
29166575	6	48	theme	anti-inflammatory	1053:1069	arg1	activities					1071:1080	the antinociceptive and anti-inflammatory activities	1029:1080	the antinociceptive and anti-inflammatory activities	1029:1080	Mice were used for the evaluation of the antinociceptive and anti-inflammatory activities.
29166575	2	49	theme	chemical	417:424	arg1	characterization					426:441	a chemical characterization	415:441	a chemical characterization	415:441	AIM OF THE STUDY In the present study, we aimed at forming a chemical characterization and investigation of the antinociceptive and anti-inflammatory activities of the aqueous extract of cashew gum without the presence of polysaccharides in its composition (CGE).
29166575	7	50	theme	pain	1172:1175	arg1	test					1177:1180	formalin-induced pain test	1155:1180	formalin-induced pain test	1155:1180	CGE was analyzed via the Irwin test, acetic acid-induced writhing test, formalin-induced pain test, and carrageenan-induced paw edema test.
29166575	19	51	theme	acids	2913:2917	arg1	presence					2891:2898	the presence	2887:2898	the presence of anacardic acids in its composition, independent of the presence of polysaccharides	2887:2984	These findings support the use of cashew gum in popular medicine and demonstrate that part of its antinociceptive and anti-inflammatory effects should also be attributed to the presence of anacardic acids in its composition, independent of the presence of polysaccharides.
29166575	5	52	theme	mass	956:959	arg1	MS/MS					975:979	MS/MS	975:979	MS/MS	975:979	The chemical characterization of CGE was performed by liquid chromatography mass spectrometry (LC-MS) and tandem mass spectrometry (MS/MS) analyses.
29166575	5	52	theme	mass	956:959	arg1	spectrometry					961:972	tandem mass spectrometry	949:972	tandem mass spectrometry (MS/MS)	949:980	The chemical characterization of CGE was performed by liquid chromatography mass spectrometry (LC-MS) and tandem mass spectrometry (MS/MS) analyses.
29166575	6	53	theme	antinociceptive	1033:1047	arg1	activities					1071:1080	the antinociceptive and anti-inflammatory activities	1029:1080	the antinociceptive and anti-inflammatory activities	1029:1080	Mice were used for the evaluation of the antinociceptive and anti-inflammatory activities.
29166575	2	54	theme	antinociceptive	468:482	arg1	activities					506:515	the antinociceptive and anti-inflammatory activities	464:515	the antinociceptive and anti-inflammatory activities of the aqueous extract of cashew gum	464:552	AIM OF THE STUDY In the present study, we aimed at forming a chemical characterization and investigation of the antinociceptive and anti-inflammatory activities of the aqueous extract of cashew gum without the presence of polysaccharides in its composition (CGE).
29166575	19	55	theme	independent	2939:2949	arg1	composition					2926:2936	its composition	2922:2936	its composition	2922:2936	These findings support the use of cashew gum in popular medicine and demonstrate that part of its antinociceptive and anti-inflammatory effects should also be attributed to the presence of anacardic acids in its composition, independent of the presence of polysaccharides.
29166575	4	56	theme	concentrated	805:816	arg1	extract					818:824	the concentrated extract	801:824	the concentrated extract	801:824	After, the acetone was removed by rotaevaporation, and the concentrated extract was lyophilized.
29166575	19	57	from	presence	2891:2898	arg1	composition					2926:2936	its composition	2922:2936	its composition	2922:2936	These findings support the use of cashew gum in popular medicine and demonstrate that part of its antinociceptive and anti-inflammatory effects should also be attributed to the presence of anacardic acids in its composition, independent of the presence of polysaccharides.
29166575	13	58	theme	antinociceptive	1995:2009	arg1	effect					2011:2016	an antinociceptive effect	1992:2016	an antinociceptive effect	1992:2016	In the formalin-induced pain test, CGE (150mg/kg, p.o.) produced an antinociceptive effect only in the first phase of the test, suggesting anti-inflammatory activity.
29166575	1	59	from	disturbances	342:353	arg1	treatment					265:273	the treatment	261:273	the treatment of inflammatory conditions	261:300	ETHNOPHARMACOLOGICAL RELEVANCE The cashew gum (Anacardium occidentale L.) is used in traditional Brazilian medicine in the treatment of inflammatory conditions, asthma, diabetes, and gastrointestinal disturbances.
29166575	9	60	theme	analgesic	1378:1386	arg1	effects					1410:1416	the analgesic and anti-inflammatory effects	1374:1416	the analgesic and anti-inflammatory effects	1374:1416	We investigated if the analgesic and anti-inflammatory effects of CGE depend of reduction in PGE2 levels, were performed the carrageenan or PGE2-induced hyperalgesia tests.
29166575	9	61	from	CGE	1421:1423	arg1	levels					1453:1458	PGE2 levels	1448:1458	PGE2 levels	1448:1458	We investigated if the analgesic and anti-inflammatory effects of CGE depend of reduction in PGE2 levels, were performed the carrageenan or PGE2-induced hyperalgesia tests.
29166575	16	62	theme	anti-inflammatory	2465:2481	arg1	effect					2483:2488	the anti-inflammatory effect	2461:2488	the anti-inflammatory effect of this extract	2461:2504	However, this dose of CGE was not able to reduce the hyperalgesia induced by PGE2, suggesting that the anti-inflammatory effect of this extract depends on the reduction in the PGE2 levels.
29166575	11	63	theme	writhing	1727:1734	arg1	number					1717:1722	the number	1713:1722	the number of writhing	1713:1734	The treatment with CGE (75, 150, and 300mg/kg, p.o.) inhibited the number of writhing in a dose-dependent manner.
29166575	9	64	theme	anti-inflammatory	1392:1408	arg1	effects					1410:1416	the analgesic and anti-inflammatory effects	1374:1416	the analgesic and anti-inflammatory effects	1374:1416	We investigated if the analgesic and anti-inflammatory effects of CGE depend of reduction in PGE2 levels, were performed the carrageenan or PGE2-induced hyperalgesia tests.
29166575	12	65	theme	chimney	1835:1841	arg1	test					1843:1846	the chimney test	1831:1846	the chimney test	1831:1846	With an intermediate dose, CGE did not cause motor impairment with the chimney test or alterations in either the open-field or sodium pentobarbital-induced sleep.
29166575	1	66	theme	Brazilian	239:247	arg1	medicine					249:256	traditional Brazilian medicine	227:256	traditional Brazilian medicine in the treatment of inflammatory conditions	227:300	ETHNOPHARMACOLOGICAL RELEVANCE The cashew gum (Anacardium occidentale L.) is used in traditional Brazilian medicine in the treatment of inflammatory conditions, asthma, diabetes, and gastrointestinal disturbances.
29166575	0	67	theme	free	75:78	arg1	polysaccharide					60:73	polysaccharide free	60:78	polysaccharide free	60:78	Chemical characterization and pharmacological assessment of polysaccharide free, standardized cashew gum extract (Anacardium occidentale L.).
29166575	0	67	theme	free	75:78	arg1	extract					105:111	standardized cashew gum extract	81:111	standardized cashew gum extract (Anacardium occidentale L.)	81:139	Chemical characterization and pharmacological assessment of polysaccharide free, standardized cashew gum extract (Anacardium occidentale L.).
29166575	10	68	theme	CGE	1569:1571	arg1	characterization					1549:1564	The chemical characterization	1536:1564	The chemical characterization of CGE	1536:1571	RESULTS The chemical characterization of CGE showed the presence of anacardic acids as the predominant phytoconstituents.
29166575	3	69	theme	polysaccharides	702:716	arg1	removal					691:697	removal	691:697	removal	691:697	MATERIALS AND METHODS The CGE was obtained after the precipitation and removal of polysaccharides through the use of acetone.
29166575	3	69	theme	polysaccharides	702:716	arg1	precipitation					673:685	precipitation	673:685	precipitation	673:685	MATERIALS AND METHODS The CGE was obtained after the precipitation and removal of polysaccharides through the use of acetone.
29166575	16	70	theme	CGE	2384:2386	arg1	dose					2376:2379	this dose	2371:2379	this dose of CGE	2371:2386	However, this dose of CGE was not able to reduce the hyperalgesia induced by PGE2, suggesting that the anti-inflammatory effect of this extract depends on the reduction in the PGE2 levels.
29166575	15	71	dep	CGE	2247:2249	arg1	p.o.					2262:2265	p.o.	2262:2265	p.o.	2262:2265	Furthermore, CGE (150mg/kg, p.o.) presented an antihyperalgic effect at all hours of the carrageenan-induced hyperalgesia test.
29166575	15	71	dep	CGE	2247:2249	arg1	150mg/kg					2252:2259	150mg/kg	2252:2259	150mg/kg	2252:2259	Furthermore, CGE (150mg/kg, p.o.) presented an antihyperalgic effect at all hours of the carrageenan-induced hyperalgesia test.
29166575	2	72	from	presence	566:573	arg1	CGE					614:616	CGE	614:616	CGE	614:616	AIM OF THE STUDY In the present study, we aimed at forming a chemical characterization and investigation of the antinociceptive and anti-inflammatory activities of the aqueous extract of cashew gum without the presence of polysaccharides in its composition (CGE).
29166575	2	72	from	presence	566:573	arg1	composition					601:611	its composition	597:611	its composition (CGE)	597:617	AIM OF THE STUDY In the present study, we aimed at forming a chemical characterization and investigation of the antinociceptive and anti-inflammatory activities of the aqueous extract of cashew gum without the presence of polysaccharides in its composition (CGE).
29166575	10	73	theme	acids	1606:1610	arg1	phytoconstituents					1631:1647	the predominant phytoconstituents	1615:1647	the predominant phytoconstituents	1615:1647	RESULTS The chemical characterization of CGE showed the presence of anacardic acids as the predominant phytoconstituents.
29166575	10	73	theme	acids	1606:1610	arg1	presence					1584:1591	the presence	1580:1591	the presence of anacardic acids	1580:1610	RESULTS The chemical characterization of CGE showed the presence of anacardic acids as the predominant phytoconstituents.
29166575	19	74	from	use	2741:2743	arg1	medicine					2770:2777	popular medicine	2762:2777	popular medicine	2762:2777	These findings support the use of cashew gum in popular medicine and demonstrate that part of its antinociceptive and anti-inflammatory effects should also be attributed to the presence of anacardic acids in its composition, independent of the presence of polysaccharides.
29166575	7	75	theme	Irwin	1108:1112	arg1	test					1114:1117	the Irwin test	1104:1117	the Irwin test	1104:1117	CGE was analyzed via the Irwin test, acetic acid-induced writhing test, formalin-induced pain test, and carrageenan-induced paw edema test.
29166575	18	76	theme	PGE2	2701:2704	arg1	levels					2706:2711	the PGE2 levels	2697:2711	the PGE2 levels	2697:2711	The action mechanisms of CGE suggest the reduction in the PGE2 levels.
29166575	9	77	theme	reduction	1435:1443	arg1	CGE					1421:1423	CGE depend	1421:1430	CGE depend of reduction in PGE2 levels	1421:1458	We investigated if the analgesic and anti-inflammatory effects of CGE depend of reduction in PGE2 levels, were performed the carrageenan or PGE2-induced hyperalgesia tests.
29166575	12	78	theme	intermediate	1772:1783	arg1	dose					1785:1788	an intermediate dose	1769:1788	an intermediate dose	1769:1788	With an intermediate dose, CGE did not cause motor impairment with the chimney test or alterations in either the open-field or sodium pentobarbital-induced sleep.
29166575	19	79	theme	gum	2755:2757	arg1	use					2741:2743	the use	2737:2743	the use of cashew gum in popular medicine	2737:2777	These findings support the use of cashew gum in popular medicine and demonstrate that part of its antinociceptive and anti-inflammatory effects should also be attributed to the presence of anacardic acids in its composition, independent of the presence of polysaccharides.
29166575	6	80	used	used	1002:1005	arg2	Mice					992:995	Mice	992:995	Mice	992:995	Mice were used for the evaluation of the antinociceptive and anti-inflammatory activities.
29166575	9	81	theme	PGE2	1448:1451	arg1	levels					1453:1458	PGE2 levels	1448:1458	PGE2 levels	1448:1458	We investigated if the analgesic and anti-inflammatory effects of CGE depend of reduction in PGE2 levels, were performed the carrageenan or PGE2-induced hyperalgesia tests.
29166575	0	82	theme	polysaccharide	60:73	arg1	characterization					9:24	Chemical characterization	0:24	Chemical characterization	0:24	Chemical characterization and pharmacological assessment of polysaccharide free, standardized cashew gum extract (Anacardium occidentale L.).
29166575	0	82	theme	polysaccharide	60:73	arg1	assessment					46:55	pharmacological assessment	30:55	pharmacological assessment	30:55	Chemical characterization and pharmacological assessment of polysaccharide free, standardized cashew gum extract (Anacardium occidentale L.).
29166575	14	83	theme	paw	2157:2159	arg1	edema					2161:2165	the carrageenan-induced paw edema	2133:2165	the carrageenan-induced paw edema	2133:2165	With the same dosage, CGE also reduced the carrageenan-induced paw edema at all hours of the test, confirming its anti-inflammatory effect.
29166575	13	84	theme	formalin-induced	1934:1949	arg1	test					1956:1959	the formalin-induced pain test	1930:1959	the formalin-induced pain test	1930:1959	In the formalin-induced pain test, CGE (150mg/kg, p.o.) produced an antinociceptive effect only in the first phase of the test, suggesting anti-inflammatory activity.
29166575	5	85	theme	CGE	876:878	arg1	characterization					856:871	The chemical characterization	843:871	The chemical characterization of CGE	843:878	The chemical characterization of CGE was performed by liquid chromatography mass spectrometry (LC-MS) and tandem mass spectrometry (MS/MS) analyses.
29166575	19	86	theme	popular	2762:2768	arg1	medicine					2770:2777	popular medicine	2762:2777	popular medicine	2762:2777	These findings support the use of cashew gum in popular medicine and demonstrate that part of its antinociceptive and anti-inflammatory effects should also be attributed to the presence of anacardic acids in its composition, independent of the presence of polysaccharides.
29166575	8	87	theme	probable	1245:1252	arg1	sedation					1254:1261	probable sedation	1245:1261	probable sedation	1245:1261	The motor activity or probable sedation was verified through the chimney, open-field, and sodium pentobarbital-induced sleep tests.
29166575	14	88	theme	carrageenan-induced	2137:2155	arg1	edema					2161:2165	the carrageenan-induced paw edema	2133:2165	the carrageenan-induced paw edema	2133:2165	With the same dosage, CGE also reduced the carrageenan-induced paw edema at all hours of the test, confirming its anti-inflammatory effect.
29166575	12	89	from	alterations	1851:1861	arg1	sleep					1920:1924	either the open-field or sodium pentobarbital-induced sleep	1866:1924	sleep	1920:1924	With an intermediate dose, CGE did not cause motor impairment with the chimney test or alterations in either the open-field or sodium pentobarbital-induced sleep.
29166575	17	90	theme	predominant	2590:2600	arg1	phytoconstituents					2602:2618	the predominant phytoconstituents	2586:2618	the predominant phytoconstituents identified in the CGE	2586:2640	CONCLUSION The anacardic acids are the predominant phytoconstituents identified in the CGE.
29166575	17	90	theme	predominant	2590:2600	arg1	acids					2576:2580	The anacardic acids	2562:2580	The anacardic acids	2562:2580	CONCLUSION The anacardic acids are the predominant phytoconstituents identified in the CGE.
29166575	5	91	theme	chromatography	904:917	arg1	spectrometry					924:935	liquid chromatography mass spectrometry	897:935	liquid chromatography mass spectrometry (LC-MS)	897:943	The chemical characterization of CGE was performed by liquid chromatography mass spectrometry (LC-MS) and tandem mass spectrometry (MS/MS) analyses.
29166575	5	91	theme	chromatography	904:917	arg1	LC-MS					938:942	LC-MS	938:942	LC-MS	938:942	The chemical characterization of CGE was performed by liquid chromatography mass spectrometry (LC-MS) and tandem mass spectrometry (MS/MS) analyses.
29166575	15	92	theme	test	2356:2359	arg1	hours					2310:2314	all hours	2306:2314	all hours of the carrageenan-induced hyperalgesia test	2306:2359	Furthermore, CGE (150mg/kg, p.o.) presented an antihyperalgic effect at all hours of the carrageenan-induced hyperalgesia test.
29166575	3	93	dep	MATERIALS	620:628	arg1	obtained					654:661	obtained	654:661	was obtained after the precipitation and removal of polysaccharides through the use of acetone	650:743	MATERIALS AND METHODS The CGE was obtained after the precipitation and removal of polysaccharides through the use of acetone.
29166575	12	94	from	test	1843:1846	arg1	sleep					1920:1924	either the open-field or sodium pentobarbital-induced sleep	1866:1924	sleep	1920:1924	With an intermediate dose, CGE did not cause motor impairment with the chimney test or alterations in either the open-field or sodium pentobarbital-induced sleep.
29166575	2	95	theme	cashew	543:548	arg1	gum					550:552	cashew gum	543:552	cashew gum	543:552	AIM OF THE STUDY In the present study, we aimed at forming a chemical characterization and investigation of the antinociceptive and anti-inflammatory activities of the aqueous extract of cashew gum without the presence of polysaccharides in its composition (CGE).
29166575	19	96	theme	anti-inflammatory	2832:2848	arg1	effects					2850:2856	its antinociceptive and anti-inflammatory effects	2808:2856	its antinociceptive and anti-inflammatory effects	2808:2856	These findings support the use of cashew gum in popular medicine and demonstrate that part of its antinociceptive and anti-inflammatory effects should also be attributed to the presence of anacardic acids in its composition, independent of the presence of polysaccharides.
29166575	12	97	dep	sleep	1920:1924	arg1	either					1866:1871	either	1866:1871	either	1866:1871	With an intermediate dose, CGE did not cause motor impairment with the chimney test or alterations in either the open-field or sodium pentobarbital-induced sleep.
29166575	15	98	theme	carrageenan-induced	2323:2341	arg1	test					2356:2359	the carrageenan-induced hyperalgesia test	2319:2359	the carrageenan-induced hyperalgesia test	2319:2359	Furthermore, CGE (150mg/kg, p.o.) presented an antihyperalgic effect at all hours of the carrageenan-induced hyperalgesia test.
29166575	9	99	theme	PGE2-induced	1495:1506	arg1	hyperalgesia					1508:1519	PGE2-induced hyperalgesia	1495:1519	PGE2-induced hyperalgesia	1495:1519	We investigated if the analgesic and anti-inflammatory effects of CGE depend of reduction in PGE2 levels, were performed the carrageenan or PGE2-induced hyperalgesia tests.
29166575	2	100	theme	polysaccharides	578:592	arg1	presence					566:573	the presence	562:573	the presence of polysaccharides in its composition (CGE)	562:617	AIM OF THE STUDY In the present study, we aimed at forming a chemical characterization and investigation of the antinociceptive and anti-inflammatory activities of the aqueous extract of cashew gum without the presence of polysaccharides in its composition (CGE).
29166575	8	101	theme	chimney	1288:1294	arg1	tests					1348:1352	the chimney, open-field, and sodium pentobarbital-induced sleep tests	1284:1352	the chimney, open-field, and sodium pentobarbital-induced sleep tests	1284:1352	The motor activity or probable sedation was verified through the chimney, open-field, and sodium pentobarbital-induced sleep tests.
29166575	16	102	from	reduction	2521:2529	arg1	levels					2543:2548	the PGE2 levels	2534:2548	the PGE2 levels	2534:2548	However, this dose of CGE was not able to reduce the hyperalgesia induced by PGE2, suggesting that the anti-inflammatory effect of this extract depends on the reduction in the PGE2 levels.
29166575	13	103	theme	anti-inflammatory	2066:2082	arg1	activity					2084:2091	anti-inflammatory activity	2066:2091	anti-inflammatory activity	2066:2091	In the formalin-induced pain test, CGE (150mg/kg, p.o.) produced an antinociceptive effect only in the first phase of the test, suggesting anti-inflammatory activity.
29166575	16	104	theme	extract	2498:2504	arg1	effect					2483:2488	the anti-inflammatory effect	2461:2488	the anti-inflammatory effect of this extract	2461:2504	However, this dose of CGE was not able to reduce the hyperalgesia induced by PGE2, suggesting that the anti-inflammatory effect of this extract depends on the reduction in the PGE2 levels.
29166575	8	105	theme	open-field	1297:1306	arg1	tests					1348:1352	the chimney, open-field, and sodium pentobarbital-induced sleep tests	1284:1352	the chimney, open-field, and sodium pentobarbital-induced sleep tests	1284:1352	The motor activity or probable sedation was verified through the chimney, open-field, and sodium pentobarbital-induced sleep tests.
29166575	18	106	theme	action	2647:2652	arg1	mechanisms					2654:2663	The action mechanisms	2643:2663	The action mechanisms of CGE	2643:2670	The action mechanisms of CGE suggest the reduction in the PGE2 levels.
29166575	0	107	theme	standardized	81:92	arg1	L					137:137	Anacardium occidentale L.	114:138	Anacardium occidentale L.	114:138	Chemical characterization and pharmacological assessment of polysaccharide free, standardized cashew gum extract (Anacardium occidentale L.).
29166575	0	107	theme	standardized	81:92	arg1	polysaccharide					60:73	polysaccharide free	60:78	polysaccharide free	60:78	Chemical characterization and pharmacological assessment of polysaccharide free, standardized cashew gum extract (Anacardium occidentale L.).
29166575	0	107	theme	standardized	81:92	arg1	extract					105:111	standardized cashew gum extract	81:111	standardized cashew gum extract (Anacardium occidentale L.)	81:139	Chemical characterization and pharmacological assessment of polysaccharide free, standardized cashew gum extract (Anacardium occidentale L.).
29166575	1	108	theme	ETHNOPHARMACOLOGICAL	142:161	arg1	RELEVANCE					163:171	ETHNOPHARMACOLOGICAL RELEVANCE The cashew gum (Anacardium occidentale L.)	142:214	ETHNOPHARMACOLOGICAL RELEVANCE The cashew gum (Anacardium occidentale L.)	142:214	ETHNOPHARMACOLOGICAL RELEVANCE The cashew gum (Anacardium occidentale L.) is used in traditional Brazilian medicine in the treatment of inflammatory conditions, asthma, diabetes, and gastrointestinal disturbances.
29166575	13	109	dep	CGE	1962:1964	arg1	p.o.					1977:1980	p.o.	1977:1980	p.o.	1977:1980	In the formalin-induced pain test, CGE (150mg/kg, p.o.) produced an antinociceptive effect only in the first phase of the test, suggesting anti-inflammatory activity.
29166575	13	109	dep	CGE	1962:1964	arg1	150mg/kg					1967:1974	150mg/kg	1967:1974	150mg/kg	1967:1974	In the formalin-induced pain test, CGE (150mg/kg, p.o.) produced an antinociceptive effect only in the first phase of the test, suggesting anti-inflammatory activity.
29166575	2	110	theme	activities	506:515	arg1	characterization					426:441	a chemical characterization	415:441	a chemical characterization	415:441	AIM OF THE STUDY In the present study, we aimed at forming a chemical characterization and investigation of the antinociceptive and anti-inflammatory activities of the aqueous extract of cashew gum without the presence of polysaccharides in its composition (CGE).
29166575	2	110	theme	activities	506:515	arg1	investigation					447:459	investigation	447:459	investigation	447:459	AIM OF THE STUDY In the present study, we aimed at forming a chemical characterization and investigation of the antinociceptive and anti-inflammatory activities of the aqueous extract of cashew gum without the presence of polysaccharides in its composition (CGE).
29166575	15	111	theme	antihyperalgic	2281:2294	arg1	effect					2296:2301	an antihyperalgic effect	2278:2301	an antihyperalgic effect	2278:2301	Furthermore, CGE (150mg/kg, p.o.) presented an antihyperalgic effect at all hours of the carrageenan-induced hyperalgesia test.
29166575	0	112	theme	gum	101:103	arg1	L					137:137	Anacardium occidentale L.	114:138	Anacardium occidentale L.	114:138	Chemical characterization and pharmacological assessment of polysaccharide free, standardized cashew gum extract (Anacardium occidentale L.).
29166575	0	112	theme	gum	101:103	arg1	polysaccharide					60:73	polysaccharide free	60:78	polysaccharide free	60:78	Chemical characterization and pharmacological assessment of polysaccharide free, standardized cashew gum extract (Anacardium occidentale L.).
29166575	0	112	theme	gum	101:103	arg1	extract					105:111	standardized cashew gum extract	81:111	standardized cashew gum extract (Anacardium occidentale L.)	81:139	Chemical characterization and pharmacological assessment of polysaccharide free, standardized cashew gum extract (Anacardium occidentale L.).
29166575	5	113	theme	liquid	897:902	arg1	spectrometry					924:935	liquid chromatography mass spectrometry	897:935	liquid chromatography mass spectrometry (LC-MS)	897:943	The chemical characterization of CGE was performed by liquid chromatography mass spectrometry (LC-MS) and tandem mass spectrometry (MS/MS) analyses.
29166575	5	113	theme	liquid	897:902	arg1	LC-MS					938:942	LC-MS	938:942	LC-MS	938:942	The chemical characterization of CGE was performed by liquid chromatography mass spectrometry (LC-MS) and tandem mass spectrometry (MS/MS) analyses.
29166575	14	114	theme	same	2103:2106	arg1	dosage					2108:2113	the same dosage	2099:2113	the same dosage	2099:2113	With the same dosage, CGE also reduced the carrageenan-induced paw edema at all hours of the test, confirming its anti-inflammatory effect.
29166575	8	115	theme	pentobarbital-induced	1320:1340	arg1	tests					1348:1352	the chimney, open-field, and sodium pentobarbital-induced sleep tests	1284:1352	the chimney, open-field, and sodium pentobarbital-induced sleep tests	1284:1352	The motor activity or probable sedation was verified through the chimney, open-field, and sodium pentobarbital-induced sleep tests.
29166575	7	116	theme	paw	1207:1209	arg1	test					1217:1220	carrageenan-induced paw edema test	1187:1220	carrageenan-induced paw edema test	1187:1220	CGE was analyzed via the Irwin test, acetic acid-induced writhing test, formalin-induced pain test, and carrageenan-induced paw edema test.
29166575	2	117	theme	extract	532:538	arg1	activities					506:515	the antinociceptive and anti-inflammatory activities	464:515	the antinociceptive and anti-inflammatory activities of the aqueous extract of cashew gum	464:552	AIM OF THE STUDY In the present study, we aimed at forming a chemical characterization and investigation of the antinociceptive and anti-inflammatory activities of the aqueous extract of cashew gum without the presence of polysaccharides in its composition (CGE).
29166575	0	118	theme	occidentale	125:135	arg1	L					137:137	Anacardium occidentale L.	114:138	Anacardium occidentale L.	114:138	Chemical characterization and pharmacological assessment of polysaccharide free, standardized cashew gum extract (Anacardium occidentale L.).
29166575	0	118	theme	occidentale	125:135	arg1	extract					105:111	standardized cashew gum extract	81:111	standardized cashew gum extract (Anacardium occidentale L.)	81:139	Chemical characterization and pharmacological assessment of polysaccharide free, standardized cashew gum extract (Anacardium occidentale L.).
29166575	12	119	theme	open-field	1877:1886	arg1	sleep					1920:1924	either the open-field or sodium pentobarbital-induced sleep	1866:1924	sleep	1920:1924	With an intermediate dose, CGE did not cause motor impairment with the chimney test or alterations in either the open-field or sodium pentobarbital-induced sleep.
29166575	5	120	dep	spectrometry	924:935	arg1	analyses					982:989	analyses	982:989	analyses	982:989	The chemical characterization of CGE was performed by liquid chromatography mass spectrometry (LC-MS) and tandem mass spectrometry (MS/MS) analyses.
29166575	6	121	theme	activities	1071:1080	arg1	evaluation					1015:1024	the evaluation	1011:1024	the evaluation of the antinociceptive and anti-inflammatory activities	1011:1080	Mice were used for the evaluation of the antinociceptive and anti-inflammatory activities.
29166575	0	122	theme	pharmacological	30:44	arg1	assessment					46:55	pharmacological assessment	30:55	pharmacological assessment	30:55	Chemical characterization and pharmacological assessment of polysaccharide free, standardized cashew gum extract (Anacardium occidentale L.).
29166575	1	123	theme	conditions	291:300	arg1	treatment					265:273	the treatment	261:273	the treatment of inflammatory conditions	261:300	ETHNOPHARMACOLOGICAL RELEVANCE The cashew gum (Anacardium occidentale L.) is used in traditional Brazilian medicine in the treatment of inflammatory conditions, asthma, diabetes, and gastrointestinal disturbances.
29166575	9	124	from	reduction	1435:1443	arg1	levels					1453:1458	PGE2 levels	1448:1458	PGE2 levels	1448:1458	We investigated if the analgesic and anti-inflammatory effects of CGE depend of reduction in PGE2 levels, were performed the carrageenan or PGE2-induced hyperalgesia tests.
29166575	5	125	theme	tandem	949:954	arg1	MS/MS					975:979	MS/MS	975:979	MS/MS	975:979	The chemical characterization of CGE was performed by liquid chromatography mass spectrometry (LC-MS) and tandem mass spectrometry (MS/MS) analyses.
29166575	5	125	theme	tandem	949:954	arg1	spectrometry					961:972	tandem mass spectrometry	949:972	tandem mass spectrometry (MS/MS)	949:980	The chemical characterization of CGE was performed by liquid chromatography mass spectrometry (LC-MS) and tandem mass spectrometry (MS/MS) analyses.
29166575	19	126	theme	presence	2958:2965	arg1	independent					2939:2949	independent	2939:2949	independent	2939:2949	These findings support the use of cashew gum in popular medicine and demonstrate that part of its antinociceptive and anti-inflammatory effects should also be attributed to the presence of anacardic acids in its composition, independent of the presence of polysaccharides.
29166575	1	127	dep	RELEVANCE	163:171	arg1	gum					184:186	The cashew gum	173:186	ETHNOPHARMACOLOGICAL RELEVANCE The cashew gum (Anacardium occidentale L.)	142:214	ETHNOPHARMACOLOGICAL RELEVANCE The cashew gum (Anacardium occidentale L.) is used in traditional Brazilian medicine in the treatment of inflammatory conditions, asthma, diabetes, and gastrointestinal disturbances.
29166575	1	127	dep	RELEVANCE	163:171	arg1	L.					212:213	L.	212:213	L.	212:213	ETHNOPHARMACOLOGICAL RELEVANCE The cashew gum (Anacardium occidentale L.) is used in traditional Brazilian medicine in the treatment of inflammatory conditions, asthma, diabetes, and gastrointestinal disturbances.
29166575	14	128	theme	anti-inflammatory	2208:2224	arg1	effect					2226:2231	its anti-inflammatory effect	2204:2231	its anti-inflammatory effect	2204:2231	With the same dosage, CGE also reduced the carrageenan-induced paw edema at all hours of the test, confirming its anti-inflammatory effect.
29166575	9	129	theme	carrageenan	1480:1490	arg1	tests					1521:1525	the carrageenan or PGE2-induced hyperalgesia tests	1476:1525	the carrageenan or PGE2-induced hyperalgesia tests	1476:1525	We investigated if the analgesic and anti-inflammatory effects of CGE depend of reduction in PGE2 levels, were performed the carrageenan or PGE2-induced hyperalgesia tests.
29166575	7	130	theme	carrageenan-induced	1187:1205	arg1	test					1217:1220	carrageenan-induced paw edema test	1187:1220	carrageenan-induced paw edema test	1187:1220	CGE was analyzed via the Irwin test, acetic acid-induced writhing test, formalin-induced pain test, and carrageenan-induced paw edema test.
29166575	7	131	theme	acid-induced	1127:1138	arg1	test					1149:1152	acetic acid-induced writhing test	1120:1152	acetic acid-induced writhing test	1120:1152	CGE was analyzed via the Irwin test, acetic acid-induced writhing test, formalin-induced pain test, and carrageenan-induced paw edema test.
29166575	10	132	theme	chemical	1540:1547	arg1	characterization					1549:1564	The chemical characterization	1536:1564	The chemical characterization of CGE	1536:1571	RESULTS The chemical characterization of CGE showed the presence of anacardic acids as the predominant phytoconstituents.
29166575	19	133	attach	presence	2891:2898	arg1	composition					2926:2936	its composition	2922:2936	its composition	2922:2936	These findings support the use of cashew gum in popular medicine and demonstrate that part of its antinociceptive and anti-inflammatory effects should also be attributed to the presence of anacardic acids in its composition, independent of the presence of polysaccharides.
29166575	19	133	attach	presence	2891:2898	arg2	acids					2913:2917	anacardic acids	2903:2917	anacardic acids	2903:2917	These findings support the use of cashew gum in popular medicine and demonstrate that part of its antinociceptive and anti-inflammatory effects should also be attributed to the presence of anacardic acids in its composition, independent of the presence of polysaccharides.
29166575	12	134	theme	motor	1809:1813	arg1	impairment					1815:1824	motor impairment	1809:1824	motor impairment	1809:1824	With an intermediate dose, CGE did not cause motor impairment with the chimney test or alterations in either the open-field or sodium pentobarbital-induced sleep.
29166575	19	135	theme	polysaccharides	2970:2984	arg1	presence					2958:2965	the presence	2954:2965	the presence of polysaccharides	2954:2984	These findings support the use of cashew gum in popular medicine and demonstrate that part of its antinociceptive and anti-inflammatory effects should also be attributed to the presence of anacardic acids in its composition, independent of the presence of polysaccharides.
29166575	1	136	theme	traditional	227:237	arg1	medicine					249:256	traditional Brazilian medicine	227:256	traditional Brazilian medicine in the treatment of inflammatory conditions	227:300	ETHNOPHARMACOLOGICAL RELEVANCE The cashew gum (Anacardium occidentale L.) is used in traditional Brazilian medicine in the treatment of inflammatory conditions, asthma, diabetes, and gastrointestinal disturbances.
29166575	11	137	with	treatment	1654:1662	arg1	CGE					1669:1671	CGE	1669:1671	CGE (75, 150, and 300mg/kg, p.o.)	1669:1701	The treatment with CGE (75, 150, and 300mg/kg, p.o.) inhibited the number of writhing in a dose-dependent manner.
29166575	15	138	attach	presented	2268:2276	arg1	hours					2310:2314	all hours	2306:2314	all hours of the carrageenan-induced hyperalgesia test	2306:2359	Furthermore, CGE (150mg/kg, p.o.) presented an antihyperalgic effect at all hours of the carrageenan-induced hyperalgesia test.
29166575	15	138	attach	presented	2268:2276	arg2	CGE					2247:2249	CGE	2247:2249	CGE (150mg/kg, p.o.)	2247:2266	Furthermore, CGE (150mg/kg, p.o.) presented an antihyperalgic effect at all hours of the carrageenan-induced hyperalgesia test.
29166575	2	139	theme	STUDY	367:371	arg1	AIM					356:358	AIM	356:358	AIM OF THE STUDY	356:371	AIM OF THE STUDY In the present study, we aimed at forming a chemical characterization and investigation of the antinociceptive and anti-inflammatory activities of the aqueous extract of cashew gum without the presence of polysaccharides in its composition (CGE).
29166575	9	140	theme	CGE	1421:1423	arg1	effects					1410:1416	the analgesic and anti-inflammatory effects	1374:1416	the analgesic and anti-inflammatory effects	1374:1416	We investigated if the analgesic and anti-inflammatory effects of CGE depend of reduction in PGE2 levels, were performed the carrageenan or PGE2-induced hyperalgesia tests.
28196780	1	0	theme	optimized	171:179	arg1	composition					190:200	the optimized chemical composition	167:200	the optimized chemical composition of biochar used as porous material for biodiesel synthesis via pseudo-catalytic transesterification	167:300	This study focuses on investigating the optimized chemical composition of biochar used as porous material for biodiesel synthesis via pseudo-catalytic transesterification.
28196780	0	1	theme	biodiesel	69:77	arg1	synthesis					79:87	biodiesel synthesis	69:87	biodiesel synthesis via pseudo-catalytic transesterification	69:128	Evaluating the effectiveness of various biochars as porous media for biodiesel synthesis via pseudo-catalytic transesterification.
28196780	1	2	theme	chemical	181:188	arg1	composition					190:200	the optimized chemical composition	167:200	the optimized chemical composition of biochar used as porous material for biodiesel synthesis via pseudo-catalytic transesterification	167:300	This study focuses on investigating the optimized chemical composition of biochar used as porous material for biodiesel synthesis via pseudo-catalytic transesterification.
28196780	0	3	theme	pseudo-catalytic	93:108	arg1	transesterification					110:128	pseudo-catalytic transesterification	93:128	pseudo-catalytic transesterification	93:128	Evaluating the effectiveness of various biochars as porous media for biodiesel synthesis via pseudo-catalytic transesterification.
28196780	5	4	theme	source	926:931	arg1	content					907:913	the lignin content	896:913	the lignin content of biomass source of biochar	896:942	The maximum achievable yield of biodiesel was sensitive to the lignin content of biomass source of biochar but not sensitive to the cellulose and hemicellulose content.
28196780	2	5	from	sources	344:350	arg1	biochars					320:327	six biochars	316:327	six biochars from different sources	316:350	To this end, six biochars from different sources were prepared and biodiesel yield obtained from pseudo-catalytic transesterification of waste cooking oil using six biochars were measured.
28196780	4	6	theme	maize	674:678	arg1	residue					680:686	maize residue	674:686	maize residue	674:686	For example, biochar generated from maize residue exhibited the best performance, which yield was reached ∼90% at 300°C; however, the maximum biodiesel yield with pine cone biochar was 43% at 380°C.
28196780	3	7	theme	biochar	629:635	arg1	material					617:624	the raw material	609:624	the raw material of biochar	609:635	Biodiesel yield and optimal reaction temperature for pseudo-catalytic transesterification were strongly dependent on the raw material of biochar.
28196780	4	8	theme	maximum	772:778	arg1	yield					790:794	the maximum biodiesel yield	768:794	the maximum biodiesel yield with pine cone biochar	768:817	For example, biochar generated from maize residue exhibited the best performance, which yield was reached ∼90% at 300°C; however, the maximum biodiesel yield with pine cone biochar was 43% at 380°C.
28196780	4	8	theme	maximum	772:778	arg1	%					825:825	43%	823:825	43%	823:825	For example, biochar generated from maize residue exhibited the best performance, which yield was reached ∼90% at 300°C; however, the maximum biodiesel yield with pine cone biochar was 43% at 380°C.
28196780	6	9	theme	pseudo-catalytic	1081:1096	arg1	material					1105:1112	pseudo-catalytic porous material	1081:1112	pseudo-catalytic porous material	1081:1112	This study provides an insight for screening the most effective biochar as pseudo-catalytic porous material, thereby helping develop more sustainable and economically viable biodiesel synthesis process.
28196780	3	10	theme	raw	613:615	arg1	material					617:624	the raw material	609:624	the raw material of biochar	609:635	Biodiesel yield and optimal reaction temperature for pseudo-catalytic transesterification were strongly dependent on the raw material of biochar.
28196780	2	11	theme	waste	440:444	arg1	transesterification					417:435	pseudo-catalytic transesterification	400:435	pseudo-catalytic transesterification of waste cooking oil	400:456	To this end, six biochars from different sources were prepared and biodiesel yield obtained from pseudo-catalytic transesterification of waste cooking oil using six biochars were measured.
28196780	3	12	theme	Biodiesel	492:500	arg1	yield					502:506	Biodiesel yield	492:506	Biodiesel yield	492:506	Biodiesel yield and optimal reaction temperature for pseudo-catalytic transesterification were strongly dependent on the raw material of biochar.
28196780	5	13	theme	biochar	936:942	arg1	source					926:931	biomass source	918:931	biomass source of biochar	918:942	The maximum achievable yield of biodiesel was sensitive to the lignin content of biomass source of biochar but not sensitive to the cellulose and hemicellulose content.
28196780	5	14	theme	lignin	900:905	arg1	content					907:913	the lignin content	896:913	the lignin content of biomass source of biochar	896:942	The maximum achievable yield of biodiesel was sensitive to the lignin content of biomass source of biochar but not sensitive to the cellulose and hemicellulose content.
28196780	5	15	theme	maximum	841:847	arg1	sensitive					883:891	sensitive	883:891	sensitive	883:891	The maximum achievable yield of biodiesel was sensitive to the lignin content of biomass source of biochar but not sensitive to the cellulose and hemicellulose content.
28196780	5	15	theme	maximum	841:847	arg1	sensitive					952:960	sensitive	952:960	sensitive	952:960	The maximum achievable yield of biodiesel was sensitive to the lignin content of biomass source of biochar but not sensitive to the cellulose and hemicellulose content.
28196780	5	15	theme	maximum	841:847	arg1	yield					860:864	The maximum achievable yield	837:864	The maximum achievable yield of biodiesel	837:877	The maximum achievable yield of biodiesel was sensitive to the lignin content of biomass source of biochar but not sensitive to the cellulose and hemicellulose content.
28196780	6	16	theme	effective	1060:1068	arg1	biochar					1070:1076	the most effective biochar	1051:1076	the most effective biochar	1051:1076	This study provides an insight for screening the most effective biochar as pseudo-catalytic porous material, thereby helping develop more sustainable and economically viable biodiesel synthesis process.
28196780	1	17	theme	biochar	205:211	arg1	composition					190:200	the optimized chemical composition	167:200	the optimized chemical composition of biochar used as porous material for biodiesel synthesis via pseudo-catalytic transesterification	167:300	This study focuses on investigating the optimized chemical composition of biochar used as porous material for biodiesel synthesis via pseudo-catalytic transesterification.
28196780	5	18	theme	achievable	849:858	arg1	sensitive					883:891	sensitive	883:891	sensitive	883:891	The maximum achievable yield of biodiesel was sensitive to the lignin content of biomass source of biochar but not sensitive to the cellulose and hemicellulose content.
28196780	5	18	theme	achievable	849:858	arg1	sensitive					952:960	sensitive	952:960	sensitive	952:960	The maximum achievable yield of biodiesel was sensitive to the lignin content of biomass source of biochar but not sensitive to the cellulose and hemicellulose content.
28196780	5	18	theme	achievable	849:858	arg1	yield					860:864	The maximum achievable yield	837:864	The maximum achievable yield of biodiesel	837:877	The maximum achievable yield of biodiesel was sensitive to the lignin content of biomass source of biochar but not sensitive to the cellulose and hemicellulose content.
28196780	5	19	theme	cellulose	969:977	arg1	content					997:1003	the cellulose and hemicellulose content	965:1003	content	997:1003	The maximum achievable yield of biodiesel was sensitive to the lignin content of biomass source of biochar but not sensitive to the cellulose and hemicellulose content.
28196780	2	20	theme	different	334:342	arg1	sources					344:350	different sources	334:350	different sources	334:350	To this end, six biochars from different sources were prepared and biodiesel yield obtained from pseudo-catalytic transesterification of waste cooking oil using six biochars were measured.
28196780	5	21	theme	hemicellulose	983:995	arg1	content					997:1003	the cellulose and hemicellulose content	965:1003	content	997:1003	The maximum achievable yield of biodiesel was sensitive to the lignin content of biomass source of biochar but not sensitive to the cellulose and hemicellulose content.
28196780	3	22	theme	reaction	520:527	arg1	temperature					529:539	optimal reaction temperature	512:539	optimal reaction temperature	512:539	Biodiesel yield and optimal reaction temperature for pseudo-catalytic transesterification were strongly dependent on the raw material of biochar.
28196780	4	23	theme	pine	801:804	arg1	biochar					811:817	pine cone biochar	801:817	pine cone biochar	801:817	For example, biochar generated from maize residue exhibited the best performance, which yield was reached ∼90% at 300°C; however, the maximum biodiesel yield with pine cone biochar was 43% at 380°C.
28196780	1	24	theme	porous	221:226	arg1	material					228:235	porous material	221:235	porous material for biodiesel synthesis via pseudo-catalytic transesterification	221:300	This study focuses on investigating the optimized chemical composition of biochar used as porous material for biodiesel synthesis via pseudo-catalytic transesterification.
28196780	0	25	theme	biochars	40:47	arg1	effectiveness					15:27	the effectiveness	11:27	the effectiveness of various biochars	11:47	Evaluating the effectiveness of various biochars as porous media for biodiesel synthesis via pseudo-catalytic transesterification.
28196780	0	25	theme	biochars	40:47	arg1	media					59:63	porous media	52:63	porous media for biodiesel synthesis via pseudo-catalytic transesterification	52:128	Evaluating the effectiveness of various biochars as porous media for biodiesel synthesis via pseudo-catalytic transesterification.
28196780	6	26	theme	biodiesel	1180:1188	arg1	process					1200:1206	more sustainable and economically viable biodiesel synthesis process	1139:1206	more sustainable and economically viable biodiesel synthesis process	1139:1206	This study provides an insight for screening the most effective biochar as pseudo-catalytic porous material, thereby helping develop more sustainable and economically viable biodiesel synthesis process.
28196780	0	27	theme	various	32:38	arg1	biochars					40:47	various biochars	32:47	various biochars	32:47	Evaluating the effectiveness of various biochars as porous media for biodiesel synthesis via pseudo-catalytic transesterification.
28196780	3	28	theme	optimal	512:518	arg1	temperature					529:539	optimal reaction temperature	512:539	optimal reaction temperature	512:539	Biodiesel yield and optimal reaction temperature for pseudo-catalytic transesterification were strongly dependent on the raw material of biochar.
28196780	0	29	theme	porous	52:57	arg1	effectiveness					15:27	the effectiveness	11:27	the effectiveness of various biochars	11:47	Evaluating the effectiveness of various biochars as porous media for biodiesel synthesis via pseudo-catalytic transesterification.
28196780	0	29	theme	porous	52:57	arg1	media					59:63	porous media	52:63	porous media for biodiesel synthesis via pseudo-catalytic transesterification	52:128	Evaluating the effectiveness of various biochars as porous media for biodiesel synthesis via pseudo-catalytic transesterification.
28196780	6	30	theme	synthesis	1190:1198	arg1	process					1200:1206	more sustainable and economically viable biodiesel synthesis process	1139:1206	more sustainable and economically viable biodiesel synthesis process	1139:1206	This study provides an insight for screening the most effective biochar as pseudo-catalytic porous material, thereby helping develop more sustainable and economically viable biodiesel synthesis process.
28196780	2	31	theme	biodiesel	370:378	arg1	yield					380:384	biodiesel yield	370:384	biodiesel yield obtained from pseudo-catalytic transesterification of waste cooking oil using six biochars	370:475	To this end, six biochars from different sources were prepared and biodiesel yield obtained from pseudo-catalytic transesterification of waste cooking oil using six biochars were measured.
28196780	4	32	with	yield	790:794	arg1	biochar					811:817	pine cone biochar	801:817	pine cone biochar	801:817	For example, biochar generated from maize residue exhibited the best performance, which yield was reached ∼90% at 300°C; however, the maximum biodiesel yield with pine cone biochar was 43% at 380°C.
28196780	5	33	dep	sensitive	883:891	arg1	sensitive					883:891	sensitive	883:891	sensitive	883:891	The maximum achievable yield of biodiesel was sensitive to the lignin content of biomass source of biochar but not sensitive to the cellulose and hemicellulose content.
28196780	5	33	dep	sensitive	883:891	arg1	yield					860:864	The maximum achievable yield	837:864	The maximum achievable yield of biodiesel	837:877	The maximum achievable yield of biodiesel was sensitive to the lignin content of biomass source of biochar but not sensitive to the cellulose and hemicellulose content.
28196780	5	33	dep	sensitive	883:891	arg1	sensitive					952:960	sensitive	952:960	sensitive	952:960	The maximum achievable yield of biodiesel was sensitive to the lignin content of biomass source of biochar but not sensitive to the cellulose and hemicellulose content.
28196780	4	34	theme	biodiesel	780:788	arg1	yield					790:794	the maximum biodiesel yield	768:794	the maximum biodiesel yield with pine cone biochar	768:817	For example, biochar generated from maize residue exhibited the best performance, which yield was reached ∼90% at 300°C; however, the maximum biodiesel yield with pine cone biochar was 43% at 380°C.
28196780	4	34	theme	biodiesel	780:788	arg1	%					825:825	43%	823:825	43%	823:825	For example, biochar generated from maize residue exhibited the best performance, which yield was reached ∼90% at 300°C; however, the maximum biodiesel yield with pine cone biochar was 43% at 380°C.
28196780	4	35	theme	cone	806:809	arg1	biochar					811:817	pine cone biochar	801:817	pine cone biochar	801:817	For example, biochar generated from maize residue exhibited the best performance, which yield was reached ∼90% at 300°C; however, the maximum biodiesel yield with pine cone biochar was 43% at 380°C.
28196780	1	36	theme	biodiesel	241:249	arg1	synthesis					251:259	biodiesel synthesis	241:259	biodiesel synthesis via pseudo-catalytic transesterification	241:300	This study focuses on investigating the optimized chemical composition of biochar used as porous material for biodiesel synthesis via pseudo-catalytic transesterification.
28196780	4	37	dep	exhibited	688:696	arg1	reached					736:742	reached	736:742	was reached ∼90% at 300°C	732:756	For example, biochar generated from maize residue exhibited the best performance, which yield was reached ∼90% at 300°C; however, the maximum biodiesel yield with pine cone biochar was 43% at 380°C.
28196780	6	38	theme	porous	1098:1103	arg1	material					1105:1112	pseudo-catalytic porous material	1081:1112	pseudo-catalytic porous material	1081:1112	This study provides an insight for screening the most effective biochar as pseudo-catalytic porous material, thereby helping develop more sustainable and economically viable biodiesel synthesis process.
28196780	3	39	theme	pseudo-catalytic	545:560	arg1	transesterification					562:580	pseudo-catalytic transesterification	545:580	pseudo-catalytic transesterification	545:580	Biodiesel yield and optimal reaction temperature for pseudo-catalytic transesterification were strongly dependent on the raw material of biochar.
28196780	6	40	theme	viable	1173:1178	arg1	process					1200:1206	more sustainable and economically viable biodiesel synthesis process	1139:1206	more sustainable and economically viable biodiesel synthesis process	1139:1206	This study provides an insight for screening the most effective biochar as pseudo-catalytic porous material, thereby helping develop more sustainable and economically viable biodiesel synthesis process.
28196780	6	41	theme	sustainable	1144:1154	arg1	process					1200:1206	more sustainable and economically viable biodiesel synthesis process	1139:1206	more sustainable and economically viable biodiesel synthesis process	1139:1206	This study provides an insight for screening the most effective biochar as pseudo-catalytic porous material, thereby helping develop more sustainable and economically viable biodiesel synthesis process.
28196780	4	42	dep	reached	736:742	arg1	which					720:724	which	720:724	which	720:724	For example, biochar generated from maize residue exhibited the best performance, which yield was reached ∼90% at 300°C; however, the maximum biodiesel yield with pine cone biochar was 43% at 380°C.
28196780	4	42	dep	reached	736:742	arg1	yield					790:794	the maximum biodiesel yield	768:794	the maximum biodiesel yield with pine cone biochar	768:817	For example, biochar generated from maize residue exhibited the best performance, which yield was reached ∼90% at 300°C; however, the maximum biodiesel yield with pine cone biochar was 43% at 380°C.
28196780	4	42	dep	reached	736:742	arg1	%					825:825	43%	823:825	43%	823:825	For example, biochar generated from maize residue exhibited the best performance, which yield was reached ∼90% at 300°C; however, the maximum biodiesel yield with pine cone biochar was 43% at 380°C.
28196780	5	43	theme	biodiesel	869:877	arg1	sensitive					883:891	sensitive	883:891	sensitive	883:891	The maximum achievable yield of biodiesel was sensitive to the lignin content of biomass source of biochar but not sensitive to the cellulose and hemicellulose content.
28196780	5	43	theme	biodiesel	869:877	arg1	sensitive					952:960	sensitive	952:960	sensitive	952:960	The maximum achievable yield of biodiesel was sensitive to the lignin content of biomass source of biochar but not sensitive to the cellulose and hemicellulose content.
28196780	5	43	theme	biodiesel	869:877	arg1	yield					860:864	The maximum achievable yield	837:864	The maximum achievable yield of biodiesel	837:877	The maximum achievable yield of biodiesel was sensitive to the lignin content of biomass source of biochar but not sensitive to the cellulose and hemicellulose content.
28196780	5	44	theme	biomass	918:924	arg1	source					926:931	biomass source	918:931	biomass source of biochar	918:942	The maximum achievable yield of biodiesel was sensitive to the lignin content of biomass source of biochar but not sensitive to the cellulose and hemicellulose content.
28196780	4	45	theme	best	702:705	arg1	performance					707:717	the best performance	698:717	the best performance	698:717	For example, biochar generated from maize residue exhibited the best performance, which yield was reached ∼90% at 300°C; however, the maximum biodiesel yield with pine cone biochar was 43% at 380°C.
28196780	4	46	from	380°C	830:834	arg1	yield					790:794	the maximum biodiesel yield	768:794	the maximum biodiesel yield with pine cone biochar	768:817	For example, biochar generated from maize residue exhibited the best performance, which yield was reached ∼90% at 300°C; however, the maximum biodiesel yield with pine cone biochar was 43% at 380°C.
28196780	4	46	from	380°C	830:834	arg1	%					825:825	43%	823:825	43%	823:825	For example, biochar generated from maize residue exhibited the best performance, which yield was reached ∼90% at 300°C; however, the maximum biodiesel yield with pine cone biochar was 43% at 380°C.
28196780	2	47	theme	pseudo-catalytic	400:415	arg1	transesterification					417:435	pseudo-catalytic transesterification	400:435	pseudo-catalytic transesterification of waste cooking oil	400:456	To this end, six biochars from different sources were prepared and biodiesel yield obtained from pseudo-catalytic transesterification of waste cooking oil using six biochars were measured.
28196780	1	48	theme	pseudo-catalytic	265:280	arg1	transesterification					282:300	pseudo-catalytic transesterification	265:300	pseudo-catalytic transesterification	265:300	This study focuses on investigating the optimized chemical composition of biochar used as porous material for biodiesel synthesis via pseudo-catalytic transesterification.
27508003	10	0	from	proteins	1526:1533	arg1	conservation					1468:1479	conservation	1468:1479	conservation of the N-glyc sites in Aspergilli homologous proteins	1468:1533	Selected proteins were evaluated for conservation of the N-glyc sites in Aspergilli homologous proteins, but a pattern of conservation was not observed.
27508003	13	1	theme	common	1864:1869	arg1	motifs					1899:1904	The most common and frequent N-glycosylated motifs	1855:1904	CONCLUSIONS The most common and frequent N-glycosylated motifs	1843:1904	CONCLUSIONS The most common and frequent N-glycosylated motifs, an overview of the N-glycosylation of the CAZymes and the number of mannoses found in N-glycans were analyzed.
27508003	15	2	theme	functions	2443:2451	arg1	understanding					2376:2388	a better understanding	2367:2388	a better understanding of glycoprotein structures, profiles, activities and functions	2367:2451	A comprehensive analysis of the protein glycosylation processes in A. nidulans will assist with a better understanding of glycoprotein structures, profiles, activities and functions.
27508003	9	3	theme	validated	1339:1347	arg1	sites					1356:1360	the validated N-glyc sites	1335:1360	the validated N-glyc sites	1335:1360	The amino acids flanking the validated N-glyc sites were mainly composed of hydrophobic and polar uncharged amino acids.
27508003	10	4	theme	Aspergilli	1504:1513	arg1	proteins					1526:1533	Aspergilli homologous proteins	1504:1533	Aspergilli homologous proteins	1504:1533	Selected proteins were evaluated for conservation of the N-glyc sites in Aspergilli homologous proteins, but a pattern of conservation was not observed.
27508003	16	5	from	optimization	2485:2496	arg1	host					2561:2564	the fungal host	2550:2564	the fungal host	2550:2564	This knowledge can help in the optimization of heterologous expression and protein secretion in the fungal host.
27508003	14	6	theme	proteins	2261:2268	arg1	secretion					2241:2249	secretion	2241:2249	secretion of target proteins	2241:2268	There are many bottlenecks in protein production by filamentous fungi, such as folding, transport by vesicles and secretion, but N-glycosylation in the correct context is a fundamental event for defining the high levels of secretion of target proteins.
27508003	10	7	theme	sites	1495:1499	arg1	conservation					1468:1479	conservation	1468:1479	conservation of the N-glyc sites in Aspergilli homologous proteins	1468:1533	Selected proteins were evaluated for conservation of the N-glyc sites in Aspergilli homologous proteins, but a pattern of conservation was not observed.
27508003	11	8	theme	global	1586:1591	arg1	analysis					1593:1600	A global analysis	1584:1600	A global analysis of N-glycans released from the proteins secreted by A. nidulans	1584:1664	A global analysis of N-glycans released from the proteins secreted by A. nidulans was also performed.
27508003	3	9	theme	N-linked	568:575	arg1	glycosylation					577:589	the N-linked glycosylation	564:589	the N-linked glycosylation of CAZymes identified in the secretome of Aspergillus nidulans grown on lignocellulose	564:676	In this study, the N-linked glycosylation of CAZymes identified in the secretome of Aspergillus nidulans grown on lignocellulose was mapped.
27508003	13	10	located	found	1984:1988	arg2	mannoses					1975:1982	mannoses	1975:1982	mannoses found in N-glycans	1975:2001	CONCLUSIONS The most common and frequent N-glycosylated motifs, an overview of the N-glycosylation of the CAZymes and the number of mannoses found in N-glycans were analyzed.
27508003	13	10	located	found	1984:1988	arg1	N-glycans					1993:2001	N-glycans	1993:2001	N-glycans	1993:2001	CONCLUSIONS The most common and frequent N-glycosylated motifs, an overview of the N-glycosylation of the CAZymes and the number of mannoses found in N-glycans were analyzed.
27508003	1	11	theme	lignocellulosic	208:222	arg1	biomass					224:230	lignocellulosic biomass	208:230	lignocellulosic biomass	208:230	BACKGROUND The genus Aspergillus includes microorganisms that naturally degrade lignocellulosic biomass, secreting large amounts of carbohydrate-active enzymes (CAZymes) that characterize their saprophyte lifestyle.
27508003	5	12	theme	265	909:911	arg1	proteins					913:920	265 proteins	909:920	265 proteins	909:920	A total of 265 proteins were identified, with 153, 210 and 182 proteins in the glucose, xylan and SCB substrates, respectively.
27508003	13	13	gly	N-glycosylated	1884:1897	arg1	motifs					1899:1904	The most common and frequent N-glycosylated motifs	1855:1904	CONCLUSIONS The most common and frequent N-glycosylated motifs	1843:1904	CONCLUSIONS The most common and frequent N-glycosylated motifs, an overview of the N-glycosylation of the CAZymes and the number of mannoses found in N-glycans were analyzed.
27508003	14	14	gly	N-glycosylation	2147:2161	arg1	context					2178:2184	the correct context	2166:2184	the correct context	2166:2184	There are many bottlenecks in protein production by filamentous fungi, such as folding, transport by vesicles and secretion, but N-glycosylation in the correct context is a fundamental event for defining the high levels of secretion of target proteins.
27508003	8	15	theme	sequon	1229:1234	arg1	N-X-T					1236:1240	the N-glyc sequon N-X-T	1218:1240	the N-glyc sequon N-X-T (72.2 %)	1218:1249	A prevalence of the N-glyc sequon N-X-T (72.2 %) was observed in N-glyc sites compared with N-X-S (27.8 %).
27508003	8	15	theme	sequon	1229:1234	arg1	%					1248:1248	72.2 %	1243:1248	72.2 %	1243:1248	A prevalence of the N-glyc sequon N-X-T (72.2 %) was observed in N-glyc sites compared with N-X-S (27.8 %).
27508003	1	16	theme	large	243:247	arg1	CAZymes					289:295	CAZymes	289:295	CAZymes	289:295	BACKGROUND The genus Aspergillus includes microorganisms that naturally degrade lignocellulosic biomass, secreting large amounts of carbohydrate-active enzymes (CAZymes) that characterize their saprophyte lifestyle.
27508003	1	16	theme	large	243:247	arg1	amounts					249:255	large amounts	243:255	large amounts of carbohydrate-active enzymes (CAZymes) that characterize their saprophyte lifestyle	243:341	BACKGROUND The genus Aspergillus includes microorganisms that naturally degrade lignocellulosic biomass, secreting large amounts of carbohydrate-active enzymes (CAZymes) that characterize their saprophyte lifestyle.
27508003	1	16	theme	large	243:247	arg1	enzymes					280:286	carbohydrate-active enzymes	260:286	carbohydrate-active enzymes (CAZymes) that characterize their saprophyte lifestyle	260:341	BACKGROUND The genus Aspergillus includes microorganisms that naturally degrade lignocellulosic biomass, secreting large amounts of carbohydrate-active enzymes (CAZymes) that characterize their saprophyte lifestyle.
27508003	16	17	theme	heterologous	2501:2512	arg1	expression					2514:2523	heterologous expression	2501:2523	heterologous expression	2501:2523	This knowledge can help in the optimization of heterologous expression and protein secretion in the fungal host.
27508003	4	18	theme	Aspergillus	698:708	arg1	RESULTS					690:696	RESULTS	690:696	RESULTS Aspergillus nidulans	690:717	RESULTS Aspergillus nidulans was grown in glucose, xylan and pretreated sugarcane bagasse (SCB) for 96 h, after which glycoproteomics and glycomics were carried out on the extracellular proteins (secretome).
27508003	10	19	theme	conservation	1553:1564	arg1	pattern					1542:1548	a pattern	1540:1548	a pattern of conservation	1540:1564	Selected proteins were evaluated for conservation of the N-glyc sites in Aspergilli homologous proteins, but a pattern of conservation was not observed.
27508003	3	20	theme	nidulans	645:652	arg1	secretome					620:628	the secretome	616:628	the secretome of Aspergillus nidulans grown on lignocellulose	616:676	In this study, the N-linked glycosylation of CAZymes identified in the secretome of Aspergillus nidulans grown on lignocellulose was mapped.
27508003	15	21	theme	better	2369:2374	arg1	understanding					2376:2388	a better understanding	2367:2388	a better understanding of glycoprotein structures, profiles, activities and functions	2367:2451	A comprehensive analysis of the protein glycosylation processes in A. nidulans will assist with a better understanding of glycoprotein structures, profiles, activities and functions.
27508003	9	22	theme	uncharged	1408:1416	arg1	acids					1424:1428	hydrophobic and polar uncharged amino acids	1386:1428	hydrophobic and polar uncharged amino acids	1386:1428	The amino acids flanking the validated N-glyc sites were mainly composed of hydrophobic and polar uncharged amino acids.
27508003	12	23	from	condition	1763:1771	arg1	proportion					1696:1705	the proportion	1692:1705	the proportion of N-glycans with Hex5 to Hex9	1692:1736	While the proportion of N-glycans with Hex5 to Hex9 was similar in the xylan condition, a prevalence of Hex5 was observed in the SCB and glucose conditions.
27508003	12	23	from	condition	1763:1771	arg1	similar					1742:1748	similar	1742:1748	similar	1742:1748	While the proportion of N-glycans with Hex5 to Hex9 was similar in the xylan condition, a prevalence of Hex5 was observed in the SCB and glucose conditions.
27508003	12	24	with	proportion	1696:1705	arg1	Hex5					1725:1728	Hex5	1725:1728	Hex5	1725:1728	While the proportion of N-glycans with Hex5 to Hex9 was similar in the xylan condition, a prevalence of Hex5 was observed in the SCB and glucose conditions.
27508003	16	25	theme	secretion	2537:2545	arg1	optimization					2485:2496	the optimization	2481:2496	the optimization of heterologous expression and protein secretion in the fungal host	2481:2564	This knowledge can help in the optimization of heterologous expression and protein secretion in the fungal host.
27508003	15	26	theme	processes	2325:2333	arg1	analysis					2287:2294	A comprehensive analysis	2271:2294	A comprehensive analysis of the protein glycosylation processes in A. nidulans	2271:2348	A comprehensive analysis of the protein glycosylation processes in A. nidulans will assist with a better understanding of glycoprotein structures, profiles, activities and functions.
27508003	2	27	theme	heterologous	526:537	arg1	proteins					539:546	heterologous proteins	526:546	heterologous proteins	526:546	Aspergillus has the capacity to perform post-translational modifications (PTM), which provides an additional advantage for the use of these organisms as a host for the production of heterologous proteins.
27508003	0	28	from	glycosylation	17:29	arg1	secretome					69:77	the secretome	65:77	the secretome of Aspergillus nidulans grown on lignocellulose	65:125	Mapping N-linked glycosylation of carbohydrate-active enzymes in the secretome of Aspergillus nidulans grown on lignocellulose.
27508003	14	29	theme	many	2028:2031	arg1	bottlenecks					2033:2043	many bottlenecks	2028:2043	many bottlenecks in protein production by filamentous fungi, such as folding, transport by vesicles and secretion	2028:2140	There are many bottlenecks in protein production by filamentous fungi, such as folding, transport by vesicles and secretion, but N-glycosylation in the correct context is a fundamental event for defining the high levels of secretion of target proteins.
27508003	14	30	theme	fundamental	2191:2201	arg1	event					2203:2207	a fundamental event	2189:2207	a fundamental event for defining the high levels of secretion of target proteins	2189:2268	There are many bottlenecks in protein production by filamentous fungi, such as folding, transport by vesicles and secretion, but N-glycosylation in the correct context is a fundamental event for defining the high levels of secretion of target proteins.
27508003	14	30	theme	fundamental	2191:2201	arg1	N-glycosylation					2147:2161	N-glycosylation	2147:2161	N-glycosylation in the correct context	2147:2184	There are many bottlenecks in protein production by filamentous fungi, such as folding, transport by vesicles and secretion, but N-glycosylation in the correct context is a fundamental event for defining the high levels of secretion of target proteins.
27508003	0	31	theme	Aspergillus	82:92	arg1	nidulans					94:101	Aspergillus nidulans	82:101	Aspergillus nidulans grown on lignocellulose	82:125	Mapping N-linked glycosylation of carbohydrate-active enzymes in the secretome of Aspergillus nidulans grown on lignocellulose.
27508003	13	32	theme	mannoses	1975:1982	arg1	overview					1910:1917	an overview	1907:1917	an overview of the N-glycosylation of the CAZymes	1907:1955	CONCLUSIONS The most common and frequent N-glycosylated motifs, an overview of the N-glycosylation of the CAZymes and the number of mannoses found in N-glycans were analyzed.
27508003	13	32	theme	mannoses	1975:1982	arg1	CONCLUSIONS					1843:1853	CONCLUSIONS	1843:1853	CONCLUSIONS The most common and frequent N-glycosylated motifs	1843:1904	CONCLUSIONS The most common and frequent N-glycosylated motifs, an overview of the N-glycosylation of the CAZymes and the number of mannoses found in N-glycans were analyzed.
27508003	13	32	theme	mannoses	1975:1982	arg1	number					1965:1970	the number	1961:1970	the number of mannoses found in N-glycans	1961:2001	CONCLUSIONS The most common and frequent N-glycosylated motifs, an overview of the N-glycosylation of the CAZymes and the number of mannoses found in N-glycans were analyzed.
27508003	12	33	theme	SCB	1815:1817	arg1	conditions					1831:1840	the SCB and glucose conditions	1811:1840	conditions	1831:1840	While the proportion of N-glycans with Hex5 to Hex9 was similar in the xylan condition, a prevalence of Hex5 was observed in the SCB and glucose conditions.
27508003	15	34	theme	activities	2428:2437	arg1	understanding					2376:2388	a better understanding	2367:2388	a better understanding of glycoprotein structures, profiles, activities and functions	2367:2451	A comprehensive analysis of the protein glycosylation processes in A. nidulans will assist with a better understanding of glycoprotein structures, profiles, activities and functions.
27508003	12	35	theme	glucose	1823:1829	arg1	conditions					1831:1840	the SCB and glucose conditions	1811:1840	conditions	1831:1840	While the proportion of N-glycans with Hex5 to Hex9 was similar in the xylan condition, a prevalence of Hex5 was observed in the SCB and glucose conditions.
27508003	15	36	theme	profiles	2418:2425	arg1	understanding					2376:2388	a better understanding	2367:2388	a better understanding of glycoprotein structures, profiles, activities and functions	2367:2451	A comprehensive analysis of the protein glycosylation processes in A. nidulans will assist with a better understanding of glycoprotein structures, profiles, activities and functions.
27508003	5	37	theme	glucose	977:983	arg1	substrates					1000:1009	the glucose, xylan and SCB substrates	973:1009	the glucose, xylan and SCB substrates	973:1009	A total of 265 proteins were identified, with 153, 210 and 182 proteins in the glucose, xylan and SCB substrates, respectively.
27508003	1	38	dep	BACKGROUND	128:137	arg1	includes					161:168	includes	161:168	includes microorganisms that naturally degrade lignocellulosic biomass, secreting large amounts of carbohydrate-active enzymes (CAZymes) that characterize their saprophyte lifestyle	161:341	BACKGROUND The genus Aspergillus includes microorganisms that naturally degrade lignocellulosic biomass, secreting large amounts of carbohydrate-active enzymes (CAZymes) that characterize their saprophyte lifestyle.
27508003	14	39	theme	correct	2170:2176	arg1	context					2178:2184	the correct context	2166:2184	the correct context	2166:2184	There are many bottlenecks in protein production by filamentous fungi, such as folding, transport by vesicles and secretion, but N-glycosylation in the correct context is a fundamental event for defining the high levels of secretion of target proteins.
27508003	15	40	theme	comprehensive	2273:2285	arg1	analysis					2287:2294	A comprehensive analysis	2271:2294	A comprehensive analysis of the protein glycosylation processes in A. nidulans	2271:2348	A comprehensive analysis of the protein glycosylation processes in A. nidulans will assist with a better understanding of glycoprotein structures, profiles, activities and functions.
27508003	0	41	theme	enzymes	54:60	arg1	glycosylation					17:29	N-linked glycosylation	8:29	N-linked glycosylation of carbohydrate-active enzymes in the secretome of Aspergillus nidulans grown on lignocellulose	8:125	Mapping N-linked glycosylation of carbohydrate-active enzymes in the secretome of Aspergillus nidulans grown on lignocellulose.
27508003	15	42	theme	structures	2406:2415	arg1	understanding					2376:2388	a better understanding	2367:2388	a better understanding of glycoprotein structures, profiles, activities and functions	2367:2451	A comprehensive analysis of the protein glycosylation processes in A. nidulans will assist with a better understanding of glycoprotein structures, profiles, activities and functions.
27508003	5	43	theme	xylan	986:990	arg1	substrates					1000:1009	the glucose, xylan and SCB substrates	973:1009	the glucose, xylan and SCB substrates	973:1009	A total of 265 proteins were identified, with 153, 210 and 182 proteins in the glucose, xylan and SCB substrates, respectively.
27508003	1	44	theme	saprophyte	322:331	arg1	lifestyle					333:341	their saprophyte lifestyle	316:341	their saprophyte lifestyle	316:341	BACKGROUND The genus Aspergillus includes microorganisms that naturally degrade lignocellulosic biomass, secreting large amounts of carbohydrate-active enzymes (CAZymes) that characterize their saprophyte lifestyle.
27508003	2	45	theme	organisms	484:492	arg1	use					471:473	the use	467:473	the use of these organisms as a host for the production of heterologous proteins	467:546	Aspergillus has the capacity to perform post-translational modifications (PTM), which provides an additional advantage for the use of these organisms as a host for the production of heterologous proteins.
27508003	10	46	theme	Selected	1431:1438	arg1	proteins					1440:1447	Selected proteins	1431:1447	Selected proteins	1431:1447	Selected proteins were evaluated for conservation of the N-glyc sites in Aspergilli homologous proteins, but a pattern of conservation was not observed.
27508003	14	47	theme	target	2254:2259	arg1	proteins					2261:2268	target proteins	2254:2268	target proteins	2254:2268	There are many bottlenecks in protein production by filamentous fungi, such as folding, transport by vesicles and secretion, but N-glycosylation in the correct context is a fundamental event for defining the high levels of secretion of target proteins.
27508003	0	48	theme	carbohydrate-active	34:52	arg1	enzymes					54:60	carbohydrate-active enzymes	34:60	carbohydrate-active enzymes	34:60	Mapping N-linked glycosylation of carbohydrate-active enzymes in the secretome of Aspergillus nidulans grown on lignocellulose.
27508003	2	49	contain	has	356:358	arg1	Aspergillus					344:354	Aspergillus	344:354	Aspergillus	344:354	Aspergillus has the capacity to perform post-translational modifications (PTM), which provides an additional advantage for the use of these organisms as a host for the production of heterologous proteins.
27508003	2	49	contain	has	356:358	arg2	capacity					364:371	the capacity to perform post-translational modifications (PTM), which provides an additional advantage for the use of these organisms as a host for the production of heterologous proteins	360:546	the capacity to perform post-translational modifications (PTM), which provides an additional advantage for the use of these organisms as a host for the production of heterologous proteins	360:546	Aspergillus has the capacity to perform post-translational modifications (PTM), which provides an additional advantage for the use of these organisms as a host for the production of heterologous proteins.
27508003	7	50	theme	sites	1137:1141	arg1	total					1108:1112	A total	1106:1112	A total of 182 N-glycosylation sites	1106:1141	A total of 182 N-glycosylation sites were identified, of which 121 were detected in 67 CAZymes.
27508003	15	51	from	processes	2325:2333	arg1	nidulans					2341:2348	A. nidulans	2338:2348	A. nidulans	2338:2348	A comprehensive analysis of the protein glycosylation processes in A. nidulans will assist with a better understanding of glycoprotein structures, profiles, activities and functions.
27508003	6	52	from	SCB	1101:1103	arg1	secretome					1078:1086	the total secretome	1068:1086	the total secretome in xylan and SCB	1068:1103	CAZymes corresponded to more than 50 % of the total secretome in xylan and SCB.
27508003	6	52	from	SCB	1101:1103	arg1	%					1063:1063	more than 50 %	1050:1063	more than 50 % of the total secretome in xylan and SCB	1050:1103	CAZymes corresponded to more than 50 % of the total secretome in xylan and SCB.
27508003	12	53	from	similar	1742:1748	arg1	condition					1763:1771	the xylan condition	1753:1771	the xylan condition	1753:1771	While the proportion of N-glycans with Hex5 to Hex9 was similar in the xylan condition, a prevalence of Hex5 was observed in the SCB and glucose conditions.
27508003	2	54	theme	post-translational	384:401	arg1	modifications					403:415	post-translational modifications	384:415	post-translational modifications (PTM)	384:421	Aspergillus has the capacity to perform post-translational modifications (PTM), which provides an additional advantage for the use of these organisms as a host for the production of heterologous proteins.
27508003	2	54	theme	post-translational	384:401	arg1	PTM					418:420	PTM	418:420	PTM	418:420	Aspergillus has the capacity to perform post-translational modifications (PTM), which provides an additional advantage for the use of these organisms as a host for the production of heterologous proteins.
27508003	1	55	theme	carbohydrate-active	260:278	arg1	CAZymes					289:295	CAZymes	289:295	CAZymes	289:295	BACKGROUND The genus Aspergillus includes microorganisms that naturally degrade lignocellulosic biomass, secreting large amounts of carbohydrate-active enzymes (CAZymes) that characterize their saprophyte lifestyle.
27508003	1	55	theme	carbohydrate-active	260:278	arg1	enzymes					280:286	carbohydrate-active enzymes	260:286	carbohydrate-active enzymes (CAZymes) that characterize their saprophyte lifestyle	260:341	BACKGROUND The genus Aspergillus includes microorganisms that naturally degrade lignocellulosic biomass, secreting large amounts of carbohydrate-active enzymes (CAZymes) that characterize their saprophyte lifestyle.
27508003	5	56	theme	SCB	996:998	arg1	substrates					1000:1009	the glucose, xylan and SCB substrates	973:1009	the glucose, xylan and SCB substrates	973:1009	A total of 265 proteins were identified, with 153, 210 and 182 proteins in the glucose, xylan and SCB substrates, respectively.
27508003	10	57	theme	N-glyc	1488:1493	arg1	sites					1495:1499	the N-glyc sites	1484:1499	the N-glyc sites in Aspergilli homologous proteins	1484:1533	Selected proteins were evaluated for conservation of the N-glyc sites in Aspergilli homologous proteins, but a pattern of conservation was not observed.
27508003	6	58	from	secretome	1078:1086	arg1	xylan					1091:1095	xylan	1091:1095	xylan	1091:1095	CAZymes corresponded to more than 50 % of the total secretome in xylan and SCB.
27508003	6	58	from	secretome	1078:1086	arg1	SCB					1101:1103	SCB	1101:1103	SCB	1101:1103	CAZymes corresponded to more than 50 % of the total secretome in xylan and SCB.
27508003	6	59	from	xylan	1091:1095	arg1	secretome					1078:1086	the total secretome	1068:1086	the total secretome in xylan and SCB	1068:1103	CAZymes corresponded to more than 50 % of the total secretome in xylan and SCB.
27508003	6	59	from	xylan	1091:1095	arg1	%					1063:1063	more than 50 %	1050:1063	more than 50 % of the total secretome in xylan and SCB	1050:1103	CAZymes corresponded to more than 50 % of the total secretome in xylan and SCB.
27508003	8	60	located	observed	1255:1262	arg2	prevalence					1204:1213	A prevalence	1202:1213	A prevalence of the N-glyc sequon N-X-T (72.2 %)	1202:1249	A prevalence of the N-glyc sequon N-X-T (72.2 %) was observed in N-glyc sites compared with N-X-S (27.8 %).
27508003	8	60	located	observed	1255:1262	arg1	sites					1274:1278	N-glyc sites	1267:1278	N-glyc sites	1267:1278	A prevalence of the N-glyc sequon N-X-T (72.2 %) was observed in N-glyc sites compared with N-X-S (27.8 %).
27508003	13	61	gly	N-glycosylation	1926:1940	arg1	CAZymes					1949:1955	the CAZymes	1945:1955	the CAZymes	1945:1955	CONCLUSIONS The most common and frequent N-glycosylated motifs, an overview of the N-glycosylation of the CAZymes and the number of mannoses found in N-glycans were analyzed.
27508003	6	62	theme	secretome	1078:1086	arg1	secretome					1078:1086	the total secretome	1068:1086	the total secretome in xylan and SCB	1068:1103	CAZymes corresponded to more than 50 % of the total secretome in xylan and SCB.
27508003	6	62	theme	secretome	1078:1086	arg1	%					1063:1063	more than 50 %	1050:1063	more than 50 % of the total secretome in xylan and SCB	1050:1103	CAZymes corresponded to more than 50 % of the total secretome in xylan and SCB.
27508003	10	63	theme	homologous	1515:1524	arg1	proteins					1526:1533	Aspergilli homologous proteins	1504:1533	Aspergilli homologous proteins	1504:1533	Selected proteins were evaluated for conservation of the N-glyc sites in Aspergilli homologous proteins, but a pattern of conservation was not observed.
27508003	6	64	from	%	1063:1063	arg1	xylan					1091:1095	xylan	1091:1095	xylan	1091:1095	CAZymes corresponded to more than 50 % of the total secretome in xylan and SCB.
27508003	6	64	from	%	1063:1063	arg1	SCB					1101:1103	SCB	1101:1103	SCB	1101:1103	CAZymes corresponded to more than 50 % of the total secretome in xylan and SCB.
27508003	9	65	theme	N-glyc	1349:1354	arg1	sites					1356:1360	the validated N-glyc sites	1335:1360	the validated N-glyc sites	1335:1360	The amino acids flanking the validated N-glyc sites were mainly composed of hydrophobic and polar uncharged amino acids.
27508003	0	66	link	N-linked	8:15	arg1	glycosylation					17:29	N-linked glycosylation	8:29	N-linked glycosylation of carbohydrate-active enzymes in the secretome of Aspergillus nidulans grown on lignocellulose	8:125	Mapping N-linked glycosylation of carbohydrate-active enzymes in the secretome of Aspergillus nidulans grown on lignocellulose.
27508003	11	67	theme	N-glycans	1605:1613	arg1	analysis					1593:1600	A global analysis	1584:1600	A global analysis of N-glycans released from the proteins secreted by A. nidulans	1584:1664	A global analysis of N-glycans released from the proteins secreted by A. nidulans was also performed.
27508003	13	68	theme	N-glycosylated	1884:1897	arg1	motifs					1899:1904	The most common and frequent N-glycosylated motifs	1855:1904	CONCLUSIONS The most common and frequent N-glycosylated motifs	1843:1904	CONCLUSIONS The most common and frequent N-glycosylated motifs, an overview of the N-glycosylation of the CAZymes and the number of mannoses found in N-glycans were analyzed.
27508003	15	69	from	nidulans	2341:2348	arg1	analysis					2287:2294	A comprehensive analysis	2271:2294	A comprehensive analysis of the protein glycosylation processes in A. nidulans	2271:2348	A comprehensive analysis of the protein glycosylation processes in A. nidulans will assist with a better understanding of glycoprotein structures, profiles, activities and functions.
27508003	14	70	theme	secretion	2241:2249	arg1	levels					2231:2236	the high levels	2222:2236	the high levels of secretion of target proteins	2222:2268	There are many bottlenecks in protein production by filamentous fungi, such as folding, transport by vesicles and secretion, but N-glycosylation in the correct context is a fundamental event for defining the high levels of secretion of target proteins.
27508003	15	71	theme	protein	2303:2309	arg1	processes					2325:2333	the protein glycosylation processes	2299:2333	the protein glycosylation processes in A. nidulans	2299:2348	A comprehensive analysis of the protein glycosylation processes in A. nidulans will assist with a better understanding of glycoprotein structures, profiles, activities and functions.
27508003	14	72	theme	filamentous	2070:2080	arg1	folding					2097:2103	folding	2097:2103	folding	2097:2103	There are many bottlenecks in protein production by filamentous fungi, such as folding, transport by vesicles and secretion, but N-glycosylation in the correct context is a fundamental event for defining the high levels of secretion of target proteins.
27508003	14	72	theme	filamentous	2070:2080	arg1	secretion					2132:2140	secretion	2132:2140	secretion	2132:2140	There are many bottlenecks in protein production by filamentous fungi, such as folding, transport by vesicles and secretion, but N-glycosylation in the correct context is a fundamental event for defining the high levels of secretion of target proteins.
27508003	14	72	theme	filamentous	2070:2080	arg1	fungi					2082:2086	filamentous fungi	2070:2086	filamentous fungi	2070:2086	There are many bottlenecks in protein production by filamentous fungi, such as folding, transport by vesicles and secretion, but N-glycosylation in the correct context is a fundamental event for defining the high levels of secretion of target proteins.
27508003	14	72	theme	filamentous	2070:2080	arg1	transport					2106:2114	transport	2106:2114	transport by vesicles	2106:2126	There are many bottlenecks in protein production by filamentous fungi, such as folding, transport by vesicles and secretion, but N-glycosylation in the correct context is a fundamental event for defining the high levels of secretion of target proteins.
27508003	3	73	theme	CAZymes	594:600	arg1	glycosylation					577:589	the N-linked glycosylation	564:589	the N-linked glycosylation of CAZymes identified in the secretome of Aspergillus nidulans grown on lignocellulose	564:676	In this study, the N-linked glycosylation of CAZymes identified in the secretome of Aspergillus nidulans grown on lignocellulose was mapped.
27508003	9	74	theme	hydrophobic	1386:1396	arg1	acids					1424:1428	hydrophobic and polar uncharged amino acids	1386:1428	hydrophobic and polar uncharged amino acids	1386:1428	The amino acids flanking the validated N-glyc sites were mainly composed of hydrophobic and polar uncharged amino acids.
27508003	16	75	theme	expression	2514:2523	arg1	optimization					2485:2496	the optimization	2481:2496	the optimization of heterologous expression and protein secretion in the fungal host	2481:2564	This knowledge can help in the optimization of heterologous expression and protein secretion in the fungal host.
27508003	8	76	theme	N-glyc	1222:1227	arg1	N-X-T					1236:1240	the N-glyc sequon N-X-T	1218:1240	the N-glyc sequon N-X-T (72.2 %)	1218:1249	A prevalence of the N-glyc sequon N-X-T (72.2 %) was observed in N-glyc sites compared with N-X-S (27.8 %).
27508003	8	76	theme	N-glyc	1222:1227	arg1	%					1248:1248	72.2 %	1243:1248	72.2 %	1243:1248	A prevalence of the N-glyc sequon N-X-T (72.2 %) was observed in N-glyc sites compared with N-X-S (27.8 %).
27508003	3	77	theme	Aspergillus	633:643	arg1	nidulans					645:652	Aspergillus nidulans	633:652	Aspergillus nidulans grown on lignocellulose	633:676	In this study, the N-linked glycosylation of CAZymes identified in the secretome of Aspergillus nidulans grown on lignocellulose was mapped.
27508003	11	78	attach	released	1615:1622	arg2	N-glycans					1605:1613	N-glycans	1605:1613	N-glycans released from the proteins secreted by A. nidulans	1605:1664	A global analysis of N-glycans released from the proteins secreted by A. nidulans was also performed.
27508003	11	78	attach	released	1615:1622	arg1	proteins					1633:1640	the proteins	1629:1640	the proteins secreted by A. nidulans	1629:1664	A global analysis of N-glycans released from the proteins secreted by A. nidulans was also performed.
27508003	9	79	theme	polar	1402:1406	arg1	acids					1424:1428	hydrophobic and polar uncharged amino acids	1386:1428	hydrophobic and polar uncharged amino acids	1386:1428	The amino acids flanking the validated N-glyc sites were mainly composed of hydrophobic and polar uncharged amino acids.
27508003	16	80	theme	protein	2529:2535	arg1	secretion					2537:2545	protein secretion	2529:2545	protein secretion	2529:2545	This knowledge can help in the optimization of heterologous expression and protein secretion in the fungal host.
27508003	8	81	theme	N-X-T	1236:1240	arg1	prevalence					1204:1213	A prevalence	1202:1213	A prevalence of the N-glyc sequon N-X-T (72.2 %)	1202:1249	A prevalence of the N-glyc sequon N-X-T (72.2 %) was observed in N-glyc sites compared with N-X-S (27.8 %).
27508003	9	82	theme	amino	1418:1422	arg1	acids					1424:1428	hydrophobic and polar uncharged amino acids	1386:1428	hydrophobic and polar uncharged amino acids	1386:1428	The amino acids flanking the validated N-glyc sites were mainly composed of hydrophobic and polar uncharged amino acids.
27508003	4	83	theme	pretreated sugarcane	751:770	arg1	bagasse 					772:779	pretreated sugarcane bagasse 	751:779	pretreated sugarcane bagasse (SCB)	751:784	RESULTS Aspergillus nidulans was grown in glucose, xylan and pretreated sugarcane bagasse (SCB) for 96 h, after which glycoproteomics and glycomics were carried out on the extracellular proteins (secretome).
27508003	4	83	theme	pretreated sugarcane	751:770	arg1	SCB					781:783	SCB	781:783	SCB	781:783	RESULTS Aspergillus nidulans was grown in glucose, xylan and pretreated sugarcane bagasse (SCB) for 96 h, after which glycoproteomics and glycomics were carried out on the extracellular proteins (secretome).
27508003	14	84	theme	high	2226:2229	arg1	levels					2231:2236	the high levels	2222:2236	the high levels of secretion of target proteins	2222:2268	There are many bottlenecks in protein production by filamentous fungi, such as folding, transport by vesicles and secretion, but N-glycosylation in the correct context is a fundamental event for defining the high levels of secretion of target proteins.
27508003	1	85	theme	genus	143:147	arg1	Aspergillus					149:159	The genus Aspergillus	139:159	The genus Aspergillus	139:159	BACKGROUND The genus Aspergillus includes microorganisms that naturally degrade lignocellulosic biomass, secreting large amounts of carbohydrate-active enzymes (CAZymes) that characterize their saprophyte lifestyle.
27508003	5	86	theme	proteins	913:920	arg1	total					900:904	A total	898:904	A total of 265 proteins	898:920	A total of 265 proteins were identified, with 153, 210 and 182 proteins in the glucose, xylan and SCB substrates, respectively.
27508003	2	87	theme	proteins	539:546	arg1	production					512:521	the production	508:521	the production of heterologous proteins	508:546	Aspergillus has the capacity to perform post-translational modifications (PTM), which provides an additional advantage for the use of these organisms as a host for the production of heterologous proteins.
27508003	3	88	gly	glycosylation	577:589	arg1	study					557:561	this study	552:561	this study	552:561	In this study, the N-linked glycosylation of CAZymes identified in the secretome of Aspergillus nidulans grown on lignocellulose was mapped.
27508003	3	88	gly	glycosylation	577:589	arg1	CAZymes					594:600	CAZymes	594:600	CAZymes identified in the secretome of Aspergillus nidulans grown on lignocellulose	594:676	In this study, the N-linked glycosylation of CAZymes identified in the secretome of Aspergillus nidulans grown on lignocellulose was mapped.
27508003	0	89	theme	nidulans	94:101	arg1	secretome					69:77	the secretome	65:77	the secretome of Aspergillus nidulans grown on lignocellulose	65:125	Mapping N-linked glycosylation of carbohydrate-active enzymes in the secretome of Aspergillus nidulans grown on lignocellulose.
27508003	8	90	theme	N-glyc	1267:1272	arg1	sites					1274:1278	N-glyc sites	1267:1278	N-glyc sites	1267:1278	A prevalence of the N-glyc sequon N-X-T (72.2 %) was observed in N-glyc sites compared with N-X-S (27.8 %).
27508003	15	91	theme	glycosylation	2311:2323	arg1	processes					2325:2333	the protein glycosylation processes	2299:2333	the protein glycosylation processes in A. nidulans	2299:2348	A comprehensive analysis of the protein glycosylation processes in A. nidulans will assist with a better understanding of glycoprotein structures, profiles, activities and functions.
27508003	13	92	dep	CONCLUSIONS	1843:1853	arg1	motifs					1899:1904	The most common and frequent N-glycosylated motifs	1855:1904	CONCLUSIONS The most common and frequent N-glycosylated motifs	1843:1904	CONCLUSIONS The most common and frequent N-glycosylated motifs, an overview of the N-glycosylation of the CAZymes and the number of mannoses found in N-glycans were analyzed.
27508003	3	93	link	N-linked	568:575	arg1	glycosylation					577:589	the N-linked glycosylation	564:589	the N-linked glycosylation of CAZymes identified in the secretome of Aspergillus nidulans grown on lignocellulose	564:676	In this study, the N-linked glycosylation of CAZymes identified in the secretome of Aspergillus nidulans grown on lignocellulose was mapped.
27508003	13	94	theme	CAZymes	1949:1955	arg1	N-glycosylation					1926:1940	the N-glycosylation	1922:1940	the N-glycosylation of the CAZymes	1922:1955	CONCLUSIONS The most common and frequent N-glycosylated motifs, an overview of the N-glycosylation of the CAZymes and the number of mannoses found in N-glycans were analyzed.
27508003	12	95	located	observed	1799:1806	arg2	prevalence					1776:1785	a prevalence	1774:1785	a prevalence of Hex5	1774:1793	While the proportion of N-glycans with Hex5 to Hex9 was similar in the xylan condition, a prevalence of Hex5 was observed in the SCB and glucose conditions.
27508003	12	95	located	observed	1799:1806	arg1	conditions					1831:1840	the SCB and glucose conditions	1811:1840	conditions	1831:1840	While the proportion of N-glycans with Hex5 to Hex9 was similar in the xylan condition, a prevalence of Hex5 was observed in the SCB and glucose conditions.
27508003	6	96	theme	total	1072:1076	arg1	secretome					1078:1086	the total secretome	1068:1086	the total secretome in xylan and SCB	1068:1103	CAZymes corresponded to more than 50 % of the total secretome in xylan and SCB.
27508003	9	97	theme	amino	1314:1318	arg1	acids					1320:1324	The amino acids	1310:1324	The amino acids flanking the validated N-glyc sites	1310:1360	The amino acids flanking the validated N-glyc sites were mainly composed of hydrophobic and polar uncharged amino acids.
27508003	2	98	theme	additional	442:451	arg1	advantage					453:461	an additional advantage	439:461	an additional advantage for the use of these organisms as a host for the production of heterologous proteins	439:546	Aspergillus has the capacity to perform post-translational modifications (PTM), which provides an additional advantage for the use of these organisms as a host for the production of heterologous proteins.
27508003	0	99	theme	N-linked	8:15	arg1	glycosylation					17:29	N-linked glycosylation	8:29	N-linked glycosylation of carbohydrate-active enzymes in the secretome of Aspergillus nidulans grown on lignocellulose	8:125	Mapping N-linked glycosylation of carbohydrate-active enzymes in the secretome of Aspergillus nidulans grown on lignocellulose.
27508003	15	100	from	analysis	2287:2294	arg1	nidulans					2341:2348	A. nidulans	2338:2348	A. nidulans	2338:2348	A comprehensive analysis of the protein glycosylation processes in A. nidulans will assist with a better understanding of glycoprotein structures, profiles, activities and functions.
27508003	15	101	theme	glycoprotein	2393:2404	arg1	structures					2406:2415	glycoprotein structures	2393:2415	glycoprotein structures	2393:2415	A comprehensive analysis of the protein glycosylation processes in A. nidulans will assist with a better understanding of glycoprotein structures, profiles, activities and functions.
27508003	7	102	theme	N-glycosylation	1121:1135	arg1	sites					1137:1141	182 N-glycosylation sites	1117:1141	182 N-glycosylation sites	1117:1141	A total of 182 N-glycosylation sites were identified, of which 121 were detected in 67 CAZymes.
27508003	12	103	theme	Hex5	1790:1793	arg1	prevalence					1776:1785	a prevalence	1774:1785	a prevalence of Hex5	1774:1793	While the proportion of N-glycans with Hex5 to Hex9 was similar in the xylan condition, a prevalence of Hex5 was observed in the SCB and glucose conditions.
27508003	10	104	from	conservation	1468:1479	arg1	proteins					1526:1533	Aspergilli homologous proteins	1504:1533	Aspergilli homologous proteins	1504:1533	Selected proteins were evaluated for conservation of the N-glyc sites in Aspergilli homologous proteins, but a pattern of conservation was not observed.
27508003	4	105	dep	Aspergillus	698:708	arg1	nidulans					710:717	nidulans	710:717	nidulans	710:717	RESULTS Aspergillus nidulans was grown in glucose, xylan and pretreated sugarcane bagasse (SCB) for 96 h, after which glycoproteomics and glycomics were carried out on the extracellular proteins (secretome).
27508003	14	106	from	N-glycosylation	2147:2161	arg1	context					2178:2184	the correct context	2166:2184	the correct context	2166:2184	There are many bottlenecks in protein production by filamentous fungi, such as folding, transport by vesicles and secretion, but N-glycosylation in the correct context is a fundamental event for defining the high levels of secretion of target proteins.
27508003	14	107	theme	protein	2048:2054	arg1	production					2056:2065	protein production	2048:2065	protein production by filamentous fungi, such as folding, transport by vesicles and secretion	2048:2140	There are many bottlenecks in protein production by filamentous fungi, such as folding, transport by vesicles and secretion, but N-glycosylation in the correct context is a fundamental event for defining the high levels of secretion of target proteins.
27508003	7	108	gly	N-glycosylation	1121:1135	arg2	sites					1137:1141	182 N-glycosylation sites	1117:1141	182 N-glycosylation sites	1117:1141	A total of 182 N-glycosylation sites were identified, of which 121 were detected in 67 CAZymes.
27508003	7	108	gly	N-glycosylation	1121:1135	arg2	182					1117:1119	182	1117:1119	182	1117:1119	A total of 182 N-glycosylation sites were identified, of which 121 were detected in 67 CAZymes.
27508003	7	109	located	detected	1178:1185	arg1	CAZymes					1193:1199	67 CAZymes	1190:1199	67 CAZymes	1190:1199	A total of 182 N-glycosylation sites were identified, of which 121 were detected in 67 CAZymes.
27508003	7	109	located	detected	1178:1185	arg2	121					1169:1171	121	1169:1171	121	1169:1171	A total of 182 N-glycosylation sites were identified, of which 121 were detected in 67 CAZymes.
27508003	12	110	theme	xylan	1757:1761	arg1	condition					1763:1771	the xylan condition	1753:1771	the xylan condition	1753:1771	While the proportion of N-glycans with Hex5 to Hex9 was similar in the xylan condition, a prevalence of Hex5 was observed in the SCB and glucose conditions.
27508003	0	111	gly	glycosylation	17:29	arg1	enzymes					54:60	carbohydrate-active enzymes	34:60	carbohydrate-active enzymes	34:60	Mapping N-linked glycosylation of carbohydrate-active enzymes in the secretome of Aspergillus nidulans grown on lignocellulose.
27508003	0	111	gly	glycosylation	17:29	arg1	secretome					69:77	the secretome	65:77	the secretome of Aspergillus nidulans grown on lignocellulose	65:125	Mapping N-linked glycosylation of carbohydrate-active enzymes in the secretome of Aspergillus nidulans grown on lignocellulose.
27508003	16	112	theme	fungal	2554:2559	arg1	host					2561:2564	the fungal host	2550:2564	the fungal host	2550:2564	This knowledge can help in the optimization of heterologous expression and protein secretion in the fungal host.
27508003	4	113	theme	extracellular	862:874	arg1	secretome					886:894	secretome	886:894	secretome	886:894	RESULTS Aspergillus nidulans was grown in glucose, xylan and pretreated sugarcane bagasse (SCB) for 96 h, after which glycoproteomics and glycomics were carried out on the extracellular proteins (secretome).
27508003	4	113	theme	extracellular	862:874	arg1	proteins					876:883	the extracellular proteins	858:883	the extracellular proteins (secretome)	858:895	RESULTS Aspergillus nidulans was grown in glucose, xylan and pretreated sugarcane bagasse (SCB) for 96 h, after which glycoproteomics and glycomics were carried out on the extracellular proteins (secretome).
27508003	13	114	theme	N-glycosylation	1926:1940	arg1	overview					1910:1917	an overview	1907:1917	an overview of the N-glycosylation of the CAZymes	1907:1955	CONCLUSIONS The most common and frequent N-glycosylated motifs, an overview of the N-glycosylation of the CAZymes and the number of mannoses found in N-glycans were analyzed.
27508003	13	114	theme	N-glycosylation	1926:1940	arg1	CONCLUSIONS					1843:1853	CONCLUSIONS	1843:1853	CONCLUSIONS The most common and frequent N-glycosylated motifs	1843:1904	CONCLUSIONS The most common and frequent N-glycosylated motifs, an overview of the N-glycosylation of the CAZymes and the number of mannoses found in N-glycans were analyzed.
27508003	13	114	theme	N-glycosylation	1926:1940	arg1	number					1965:1970	the number	1961:1970	the number of mannoses found in N-glycans	1961:2001	CONCLUSIONS The most common and frequent N-glycosylated motifs, an overview of the N-glycosylation of the CAZymes and the number of mannoses found in N-glycans were analyzed.
27508003	5	115	from	proteins	961:968	arg1	substrates					1000:1009	the glucose, xylan and SCB substrates	973:1009	the glucose, xylan and SCB substrates	973:1009	A total of 265 proteins were identified, with 153, 210 and 182 proteins in the glucose, xylan and SCB substrates, respectively.
27508003	10	116	from	sites	1495:1499	arg1	proteins					1526:1533	Aspergilli homologous proteins	1504:1533	Aspergilli homologous proteins	1504:1533	Selected proteins were evaluated for conservation of the N-glyc sites in Aspergilli homologous proteins, but a pattern of conservation was not observed.
27508003	1	117	theme	enzymes	280:286	arg1	CAZymes					289:295	CAZymes	289:295	CAZymes	289:295	BACKGROUND The genus Aspergillus includes microorganisms that naturally degrade lignocellulosic biomass, secreting large amounts of carbohydrate-active enzymes (CAZymes) that characterize their saprophyte lifestyle.
27508003	1	117	theme	enzymes	280:286	arg1	amounts					249:255	large amounts	243:255	large amounts of carbohydrate-active enzymes (CAZymes) that characterize their saprophyte lifestyle	243:341	BACKGROUND The genus Aspergillus includes microorganisms that naturally degrade lignocellulosic biomass, secreting large amounts of carbohydrate-active enzymes (CAZymes) that characterize their saprophyte lifestyle.
27508003	1	117	theme	enzymes	280:286	arg1	enzymes					280:286	carbohydrate-active enzymes	260:286	carbohydrate-active enzymes (CAZymes) that characterize their saprophyte lifestyle	260:341	BACKGROUND The genus Aspergillus includes microorganisms that naturally degrade lignocellulosic biomass, secreting large amounts of carbohydrate-active enzymes (CAZymes) that characterize their saprophyte lifestyle.
27508003	12	118	theme	N-glycans	1710:1718	arg1	similar					1742:1748	similar	1742:1748	similar	1742:1748	While the proportion of N-glycans with Hex5 to Hex9 was similar in the xylan condition, a prevalence of Hex5 was observed in the SCB and glucose conditions.
27508003	12	118	theme	N-glycans	1710:1718	arg1	proportion					1696:1705	the proportion	1692:1705	the proportion of N-glycans with Hex5 to Hex9	1692:1736	While the proportion of N-glycans with Hex5 to Hex9 was similar in the xylan condition, a prevalence of Hex5 was observed in the SCB and glucose conditions.
27508003	15	119	gly	glycoprotein	2393:2404	arg1	glycoprotein					2393:2404	glycoprotein structures	2393:2415	glycoprotein structures	2393:2415	A comprehensive analysis of the protein glycosylation processes in A. nidulans will assist with a better understanding of glycoprotein structures, profiles, activities and functions.
27508003	14	120	from	bottlenecks	2033:2043	arg1	production					2056:2065	protein production	2048:2065	protein production by filamentous fungi, such as folding, transport by vesicles and secretion	2048:2140	There are many bottlenecks in protein production by filamentous fungi, such as folding, transport by vesicles and secretion, but N-glycosylation in the correct context is a fundamental event for defining the high levels of secretion of target proteins.
27035484	5	0	theme	pyrolysis	788:796	arg1	behavior					798:805	the pyrolysis behavior	784:805	the pyrolysis behavior of the pine sawdust	784:825	It was found that the model is capable of predicting the pyrolysis behavior of the pine sawdust.
27035484	7	1	theme	evolution	990:998	arg1	profiles					1000:1007	The evolution profiles	986:1007	The evolution profiles of H2, CH4, CO, and CO2	986:1031	The evolution profiles of H2, CH4, CO, and CO2 were well predicted using the three-parallel Gaussian distribution model.
27035484	4	2	theme	first	612:616	arg1	pseudocomponents					636:651	The first, second and third pseudocomponents	608:651	The first, second and third pseudocomponents	608:651	The first, second and third pseudocomponents represent the fractions of hemicellulose, cellulose and lignin, respectively.
27035484	8	3	theme	chemical	1124:1131	arg1	composition					1133:1143	the chemical composition	1120:1143	the chemical composition of bio-oil	1120:1154	In addition, the chemical composition of bio-oil was also obtained by pyrolysis-gas chromatography/mass spectrometry instrument (Py-GC/MS).
27035484	6	4	theme	energy	843:848	arg1	peaks					863:867	The activation energy distribution peaks	828:867	The activation energy distribution peaks for the three pseudo-components	828:899	The activation energy distribution peaks for the three pseudo-components were centered at 186.8, 197.5 and 203.9kJmol(-1) for the pine sawdust, respectively.
27035484	1	5	theme	pyrolysis	165:173	arg1	productions					150:160	The kinetic and energy productions	127:160	productions	150:160	The kinetic and energy productions of pyrolysis of a lignocellulosic biomass were investigated using a three-parallel Gaussian distribution method in this work.
27035484	0	6	theme	Gaussian	102:109	arg1	model					120:124	a three-parallel Gaussian reaction model	85:124	a three-parallel Gaussian reaction model	85:124	Kinetic and energy production analysis of pyrolysis of lignocellulosic biomass using a three-parallel Gaussian reaction model.
27035484	6	7	theme	activation	832:841	arg1	peaks					863:867	The activation energy distribution peaks	828:867	The activation energy distribution peaks for the three pseudo-components	828:899	The activation energy distribution peaks for the three pseudo-components were centered at 186.8, 197.5 and 203.9kJmol(-1) for the pine sawdust, respectively.
27035484	5	8	theme	pine	814:817	arg1	sawdust					819:825	the pine sawdust	810:825	the pine sawdust	810:825	It was found that the model is capable of predicting the pyrolysis behavior of the pine sawdust.
27035484	4	9	theme	second	619:624	arg1	pseudocomponents					636:651	The first, second and third pseudocomponents	608:651	The first, second and third pseudocomponents	608:651	The first, second and third pseudocomponents represent the fractions of hemicellulose, cellulose and lignin, respectively.
27035484	0	10	theme	three-parallel	87:100	arg1	model					120:124	a three-parallel Gaussian reaction model	85:124	a three-parallel Gaussian reaction model	85:124	Kinetic and energy production analysis of pyrolysis of lignocellulosic biomass using a three-parallel Gaussian reaction model.
27035484	3	11	theme	thermal	514:520	arg1	behaviors					536:544	thermal decomposition behaviors	514:544	thermal decomposition behaviors of the three components, hemicellulose, cellulose and lignin	514:605	A three-parallel Gaussian distributed activation energy model (DAEM)-reaction model was used to describe thermal decomposition behaviors of the three components, hemicellulose, cellulose and lignin.
27035484	7	12	theme	Gaussian	1078:1085	arg1	model					1100:1104	the three-parallel Gaussian distribution model	1059:1104	the three-parallel Gaussian distribution model	1059:1104	The evolution profiles of H2, CH4, CO, and CO2 were well predicted using the three-parallel Gaussian distribution model.
27035484	2	13	theme	pyrolysis	292:300	arg1	experiment					302:311	The pyrolysis experiment	288:311	The pyrolysis experiment of the pine sawdust	288:331	The pyrolysis experiment of the pine sawdust was performed using a thermogravimetric-mass spectroscopy (TG-MS) analyzer.
27035484	4	14	theme	lignin	709:714	arg1	fractions					667:675	the fractions	663:675	the fractions of hemicellulose, cellulose and lignin	663:714	The first, second and third pseudocomponents represent the fractions of hemicellulose, cellulose and lignin, respectively.
27035484	4	15	theme	cellulose	695:703	arg1	fractions					667:675	the fractions	663:675	the fractions of hemicellulose, cellulose and lignin	663:714	The first, second and third pseudocomponents represent the fractions of hemicellulose, cellulose and lignin, respectively.
27035484	0	16	theme	reaction	111:118	arg1	model					120:124	a three-parallel Gaussian reaction model	85:124	a three-parallel Gaussian reaction model	85:124	Kinetic and energy production analysis of pyrolysis of lignocellulosic biomass using a three-parallel Gaussian reaction model.
27035484	7	17	theme	distribution	1087:1098	arg1	model					1100:1104	the three-parallel Gaussian distribution model	1059:1104	the three-parallel Gaussian distribution model	1059:1104	The evolution profiles of H2, CH4, CO, and CO2 were well predicted using the three-parallel Gaussian distribution model.
27035484	3	18	theme	three-parallel	411:424	arg1	model					487:491	A three-parallel Gaussian distributed activation energy model (DAEM)-reaction model	409:491	A three-parallel Gaussian distributed activation energy model (DAEM)-reaction model	409:491	A three-parallel Gaussian distributed activation energy model (DAEM)-reaction model was used to describe thermal decomposition behaviors of the three components, hemicellulose, cellulose and lignin.
27035484	3	19	theme	distributed	435:445	arg1	model					487:491	A three-parallel Gaussian distributed activation energy model (DAEM)-reaction model	409:491	A three-parallel Gaussian distributed activation energy model (DAEM)-reaction model	409:491	A three-parallel Gaussian distributed activation energy model (DAEM)-reaction model was used to describe thermal decomposition behaviors of the three components, hemicellulose, cellulose and lignin.
27035484	1	20	theme	lignocellulosic	180:194	arg1	biomass					196:202	a lignocellulosic biomass	178:202	a lignocellulosic biomass	178:202	The kinetic and energy productions of pyrolysis of a lignocellulosic biomass were investigated using a three-parallel Gaussian distribution method in this work.
27035484	0	21	theme	Kinetic	0:6	arg1	analysis					30:37	Kinetic and energy production analysis	0:37	analysis	30:37	Kinetic and energy production analysis of pyrolysis of lignocellulosic biomass using a three-parallel Gaussian reaction model.
27035484	7	22	theme	three-parallel	1063:1076	arg1	model					1100:1104	the three-parallel Gaussian distribution model	1059:1104	the three-parallel Gaussian distribution model	1059:1104	The evolution profiles of H2, CH4, CO, and CO2 were well predicted using the three-parallel Gaussian distribution model.
27035484	2	23	theme	sawdust	325:331	arg1	experiment					302:311	The pyrolysis experiment	288:311	The pyrolysis experiment of the pine sawdust	288:331	The pyrolysis experiment of the pine sawdust was performed using a thermogravimetric-mass spectroscopy (TG-MS) analyzer.
27035484	1	24	theme	biomass	196:202	arg1	pyrolysis					165:173	pyrolysis	165:173	pyrolysis of a lignocellulosic biomass	165:202	The kinetic and energy productions of pyrolysis of a lignocellulosic biomass were investigated using a three-parallel Gaussian distribution method in this work.
27035484	0	25	theme	production	19:28	arg1	analysis					30:37	Kinetic and energy production analysis	0:37	analysis	30:37	Kinetic and energy production analysis of pyrolysis of lignocellulosic biomass using a three-parallel Gaussian reaction model.
27035484	2	26	theme	pine	320:323	arg1	sawdust					325:331	the pine sawdust	316:331	the pine sawdust	316:331	The pyrolysis experiment of the pine sawdust was performed using a thermogravimetric-mass spectroscopy (TG-MS) analyzer.
27035484	0	27	theme	energy	12:17	arg1	analysis					30:37	Kinetic and energy production analysis	0:37	analysis	30:37	Kinetic and energy production analysis of pyrolysis of lignocellulosic biomass using a three-parallel Gaussian reaction model.
27035484	3	28	theme	components	559:568	arg1	behaviors					536:544	thermal decomposition behaviors	514:544	thermal decomposition behaviors of the three components, hemicellulose, cellulose and lignin	514:605	A three-parallel Gaussian distributed activation energy model (DAEM)-reaction model was used to describe thermal decomposition behaviors of the three components, hemicellulose, cellulose and lignin.
27035484	2	29	theme	spectroscopy	378:389	arg1	analyzer					399:406	a thermogravimetric-mass spectroscopy (TG-MS) analyzer	353:406	a thermogravimetric-mass spectroscopy (TG-MS) analyzer	353:406	The pyrolysis experiment of the pine sawdust was performed using a thermogravimetric-mass spectroscopy (TG-MS) analyzer.
27035484	8	30	theme	instrument	1224:1233	arg1	spectrometry					1211:1222	pyrolysis-gas chromatography/mass spectrometry instrument	1177:1233	pyrolysis-gas chromatography/mass spectrometry instrument (Py-GC/MS)	1177:1244	In addition, the chemical composition of bio-oil was also obtained by pyrolysis-gas chromatography/mass spectrometry instrument (Py-GC/MS).
27035484	8	30	theme	instrument	1224:1233	arg1	Py-GC/MS					1236:1243	Py-GC/MS	1236:1243	Py-GC/MS	1236:1243	In addition, the chemical composition of bio-oil was also obtained by pyrolysis-gas chromatography/mass spectrometry instrument (Py-GC/MS).
27035484	4	31	theme	hemicellulose	680:692	arg1	fractions					667:675	the fractions	663:675	the fractions of hemicellulose, cellulose and lignin	663:714	The first, second and third pseudocomponents represent the fractions of hemicellulose, cellulose and lignin, respectively.
27035484	3	32	theme	-reaction	477:485	arg1	model					487:491	A three-parallel Gaussian distributed activation energy model (DAEM)-reaction model	409:491	A three-parallel Gaussian distributed activation energy model (DAEM)-reaction model	409:491	A three-parallel Gaussian distributed activation energy model (DAEM)-reaction model was used to describe thermal decomposition behaviors of the three components, hemicellulose, cellulose and lignin.
27035484	6	33	theme	pine	958:961	arg1	sawdust					963:969	the pine sawdust	954:969	the pine sawdust	954:969	The activation energy distribution peaks for the three pseudo-components were centered at 186.8, 197.5 and 203.9kJmol(-1) for the pine sawdust, respectively.
27035484	8	34	theme	chromatography/mass	1191:1209	arg1	spectrometry					1211:1222	pyrolysis-gas chromatography/mass spectrometry instrument	1177:1233	pyrolysis-gas chromatography/mass spectrometry instrument (Py-GC/MS)	1177:1244	In addition, the chemical composition of bio-oil was also obtained by pyrolysis-gas chromatography/mass spectrometry instrument (Py-GC/MS).
27035484	8	34	theme	chromatography/mass	1191:1209	arg1	Py-GC/MS					1236:1243	Py-GC/MS	1236:1243	Py-GC/MS	1236:1243	In addition, the chemical composition of bio-oil was also obtained by pyrolysis-gas chromatography/mass spectrometry instrument (Py-GC/MS).
27035484	7	35	theme	CH4	1016:1018	arg1	profiles					1000:1007	The evolution profiles	986:1007	The evolution profiles of H2, CH4, CO, and CO2	986:1031	The evolution profiles of H2, CH4, CO, and CO2 were well predicted using the three-parallel Gaussian distribution model.
27035484	3	36	theme	Gaussian	426:433	arg1	model					487:491	A three-parallel Gaussian distributed activation energy model (DAEM)-reaction model	409:491	A three-parallel Gaussian distributed activation energy model (DAEM)-reaction model	409:491	A three-parallel Gaussian distributed activation energy model (DAEM)-reaction model was used to describe thermal decomposition behaviors of the three components, hemicellulose, cellulose and lignin.
27035484	8	37	theme	bio-oil	1148:1154	arg1	composition					1133:1143	the chemical composition	1120:1143	the chemical composition of bio-oil	1120:1154	In addition, the chemical composition of bio-oil was also obtained by pyrolysis-gas chromatography/mass spectrometry instrument (Py-GC/MS).
27035484	5	38	theme	sawdust	819:825	arg1	behavior					798:805	the pyrolysis behavior	784:805	the pyrolysis behavior of the pine sawdust	784:825	It was found that the model is capable of predicting the pyrolysis behavior of the pine sawdust.
27035484	2	39	theme	thermogravimetric-mass	355:376	arg1	TG-MS					392:396	TG-MS	392:396	TG-MS	392:396	The pyrolysis experiment of the pine sawdust was performed using a thermogravimetric-mass spectroscopy (TG-MS) analyzer.
27035484	2	39	theme	thermogravimetric-mass	355:376	arg1	spectroscopy					378:389	thermogravimetric-mass spectroscopy	355:389	a thermogravimetric-mass spectroscopy (TG-MS) analyzer	353:406	The pyrolysis experiment of the pine sawdust was performed using a thermogravimetric-mass spectroscopy (TG-MS) analyzer.
27035484	0	40	theme	pyrolysis	42:50	arg1	analysis					30:37	Kinetic and energy production analysis	0:37	analysis	30:37	Kinetic and energy production analysis of pyrolysis of lignocellulosic biomass using a three-parallel Gaussian reaction model.
27035484	7	41	theme	CO2	1029:1031	arg1	profiles					1000:1007	The evolution profiles	986:1007	The evolution profiles of H2, CH4, CO, and CO2	986:1031	The evolution profiles of H2, CH4, CO, and CO2 were well predicted using the three-parallel Gaussian distribution model.
27035484	7	42	theme	H2	1012:1013	arg1	profiles					1000:1007	The evolution profiles	986:1007	The evolution profiles of H2, CH4, CO, and CO2	986:1031	The evolution profiles of H2, CH4, CO, and CO2 were well predicted using the three-parallel Gaussian distribution model.
27035484	3	43	theme	decomposition	522:534	arg1	behaviors					536:544	thermal decomposition behaviors	514:544	thermal decomposition behaviors of the three components, hemicellulose, cellulose and lignin	514:605	A three-parallel Gaussian distributed activation energy model (DAEM)-reaction model was used to describe thermal decomposition behaviors of the three components, hemicellulose, cellulose and lignin.
27035484	7	44	theme	CO	1021:1022	arg1	profiles					1000:1007	The evolution profiles	986:1007	The evolution profiles of H2, CH4, CO, and CO2	986:1031	The evolution profiles of H2, CH4, CO, and CO2 were well predicted using the three-parallel Gaussian distribution model.
27035484	1	45	theme	kinetic	131:137	arg1	productions					150:160	The kinetic and energy productions	127:160	productions	150:160	The kinetic and energy productions of pyrolysis of a lignocellulosic biomass were investigated using a three-parallel Gaussian distribution method in this work.
27035484	3	46	used	used	497:500	arg2	model					487:491	A three-parallel Gaussian distributed activation energy model (DAEM)-reaction model	409:491	A three-parallel Gaussian distributed activation energy model (DAEM)-reaction model	409:491	A three-parallel Gaussian distributed activation energy model (DAEM)-reaction model was used to describe thermal decomposition behaviors of the three components, hemicellulose, cellulose and lignin.
27035484	1	47	theme	three-parallel	230:243	arg1	method					267:272	a three-parallel Gaussian distribution method	228:272	a three-parallel Gaussian distribution method	228:272	The kinetic and energy productions of pyrolysis of a lignocellulosic biomass were investigated using a three-parallel Gaussian distribution method in this work.
27035484	1	48	theme	Gaussian	245:252	arg1	method					267:272	a three-parallel Gaussian distribution method	228:272	a three-parallel Gaussian distribution method	228:272	The kinetic and energy productions of pyrolysis of a lignocellulosic biomass were investigated using a three-parallel Gaussian distribution method in this work.
27035484	0	49	theme	biomass	71:77	arg1	pyrolysis					42:50	pyrolysis	42:50	pyrolysis of lignocellulosic biomass	42:77	Kinetic and energy production analysis of pyrolysis of lignocellulosic biomass using a three-parallel Gaussian reaction model.
27035484	6	50	theme	distribution	850:861	arg1	peaks					863:867	The activation energy distribution peaks	828:867	The activation energy distribution peaks for the three pseudo-components	828:899	The activation energy distribution peaks for the three pseudo-components were centered at 186.8, 197.5 and 203.9kJmol(-1) for the pine sawdust, respectively.
27035484	1	51	theme	energy	143:148	arg1	productions					150:160	The kinetic and energy productions	127:160	productions	150:160	The kinetic and energy productions of pyrolysis of a lignocellulosic biomass were investigated using a three-parallel Gaussian distribution method in this work.
27035484	1	52	theme	distribution	254:265	arg1	method					267:272	a three-parallel Gaussian distribution method	228:272	a three-parallel Gaussian distribution method	228:272	The kinetic and energy productions of pyrolysis of a lignocellulosic biomass were investigated using a three-parallel Gaussian distribution method in this work.
27035484	0	53	theme	lignocellulosic	55:69	arg1	biomass					71:77	lignocellulosic biomass	55:77	lignocellulosic biomass	55:77	Kinetic and energy production analysis of pyrolysis of lignocellulosic biomass using a three-parallel Gaussian reaction model.
27035484	8	54	theme	pyrolysis-gas	1177:1189	arg1	spectrometry					1211:1222	pyrolysis-gas chromatography/mass spectrometry instrument	1177:1233	pyrolysis-gas chromatography/mass spectrometry instrument (Py-GC/MS)	1177:1244	In addition, the chemical composition of bio-oil was also obtained by pyrolysis-gas chromatography/mass spectrometry instrument (Py-GC/MS).
27035484	8	54	theme	pyrolysis-gas	1177:1189	arg1	Py-GC/MS					1236:1243	Py-GC/MS	1236:1243	Py-GC/MS	1236:1243	In addition, the chemical composition of bio-oil was also obtained by pyrolysis-gas chromatography/mass spectrometry instrument (Py-GC/MS).
27035484	4	55	theme	third	630:634	arg1	pseudocomponents					636:651	The first, second and third pseudocomponents	608:651	The first, second and third pseudocomponents	608:651	The first, second and third pseudocomponents represent the fractions of hemicellulose, cellulose and lignin, respectively.
26524992	13	0	theme	copper	1870:1875	arg1	silver					1860:1865	silver	1860:1865	silver	1860:1865	The amount of silver or copper released daily by composite membranes represented roughly 1% of their total load of metals.
26524992	13	0	theme	copper	1870:1875	arg1	amount					1850:1855	The amount	1846:1855	The amount of silver or copper released daily by composite membranes	1846:1913	The amount of silver or copper released daily by composite membranes represented roughly 1% of their total load of metals.
26524992	13	0	theme	copper	1870:1875	arg1	copper					1870:1875	copper	1870:1875	copper	1870:1875	The amount of silver or copper released daily by composite membranes represented roughly 1% of their total load of metals.
26524992	9	1	with	different	1431:1439	arg1	reductions					1470:1479	average reductions	1462:1479	average reductions around 70% for bare particles and 50% for membranes	1462:1531	The effect of copper and silver loaded materials was not significantly different from each other with average reductions around 70% for bare particles and 50% for membranes.
26524992	1	2	theme	Aspergillus	306:316	arg1	niger					318:322	the fungus Aspergillus niger	295:322	the fungus Aspergillus niger	295:322	Electrospun cellulose acetate composites containing silver and copper nanoparticles supported in sepiolite and mesoporous silica were prepared and tested as fungistatic membranes against the fungus Aspergillus niger.
26524992	5	3	dep	incubated	924:932	arg1	included					977:984	included	977:984	included in cellulose acetate composite membranes	977:1025	The results showed that silver and copper nanomaterials significantly impaired the growth of fungi when the spores were incubated either in direct contact with particles or included in cellulose acetate composite membranes.
26524992	5	3	dep	incubated	924:932	arg1	either					934:939	either	934:939	either	934:939	The results showed that silver and copper nanomaterials significantly impaired the growth of fungi when the spores were incubated either in direct contact with particles or included in cellulose acetate composite membranes.
26524992	3	4	theme	micrometer	571:580	arg1	range					582:586	the micrometer range	567:586	the micrometer range	567:586	Sepiolite and silica were well dispersed within the fibers, with larger aggregates in the micrometer range, and allowed a controlled release of metals to create a fungistatic environment.
26524992	10	5	theme	activity	1610:1617	arg1	decrease					1588:1595	a decrease	1586:1595	a decrease of metabolic activity of up to 80% with respect to controls	1586:1655	Copper on sepiolite was particularly efficient with a decrease of metabolic activity of up to 80% with respect to controls.
26524992	10	6	dep	80	1628:1629	arg1	to					1625:1626	to	1625:1626	to	1625:1626	Copper on sepiolite was particularly efficient with a decrease of metabolic activity of up to 80% with respect to controls.
26524992	1	7	theme	mesoporous	219:228	arg1	silica					230:235	mesoporous silica	219:235	mesoporous silica	219:235	Electrospun cellulose acetate composites containing silver and copper nanoparticles supported in sepiolite and mesoporous silica were prepared and tested as fungistatic membranes against the fungus Aspergillus niger.
26524992	5	8	theme	fungi	897:901	arg1	growth					887:892	the growth	883:892	the growth of fungi	883:901	The results showed that silver and copper nanomaterials significantly impaired the growth of fungi when the spores were incubated either in direct contact with particles or included in cellulose acetate composite membranes.
26524992	8	9	theme	Growth	1255:1260	arg1	reduction					1262:1270	Growth reduction	1255:1270	Growth reduction	1255:1270	Growth reduction was important for all the particles and membranes with respect to non-exposed controls.
26524992	8	10	dep	particles	1298:1306	arg1	all					1290:1292	all	1290:1292	all	1290:1292	Growth reduction was important for all the particles and membranes with respect to non-exposed controls.
26524992	8	10	dep	particles	1298:1306	arg1	the					1294:1296	the	1294:1296	the	1294:1296	Growth reduction was important for all the particles and membranes with respect to non-exposed controls.
26524992	1	11	theme	silver	160:165	arg1	nanoparticles					178:190	silver and copper nanoparticles	160:190	silver and copper nanoparticles supported in sepiolite and mesoporous silica	160:235	Electrospun cellulose acetate composites containing silver and copper nanoparticles supported in sepiolite and mesoporous silica were prepared and tested as fungistatic membranes against the fungus Aspergillus niger.
26524992	4	12	theme	fungal	734:739	arg1	rate					748:751	fungal growth rate	734:751	fungal growth rate	734:751	The effect was assessed using digital image analysis to evaluate fungal growth rate and fluorescence readings using a viability stain.
26524992	3	13	theme	controlled	603:612	arg1	release					614:620	a controlled release	601:620	a controlled release of metals	601:630	Sepiolite and silica were well dispersed within the fibers, with larger aggregates in the micrometer range, and allowed a controlled release of metals to create a fungistatic environment.
26524992	5	14	theme	direct	944:949	arg1	contact					951:957	direct contact	944:957	direct contact with particles	944:972	The results showed that silver and copper nanomaterials significantly impaired the growth of fungi when the spores were incubated either in direct contact with particles or included in cellulose acetate composite membranes.
26524992	1	15	theme	copper	171:176	arg1	nanoparticles					178:190	silver and copper nanoparticles	160:190	silver and copper nanoparticles supported in sepiolite and mesoporous silica	160:235	Electrospun cellulose acetate composites containing silver and copper nanoparticles supported in sepiolite and mesoporous silica were prepared and tested as fungistatic membranes against the fungus Aspergillus niger.
26524992	4	16	theme	image	707:711	arg1	analysis					713:720	digital image analysis	699:720	digital image analysis	699:720	The effect was assessed using digital image analysis to evaluate fungal growth rate and fluorescence readings using a viability stain.
26524992	14	17	theme	antifungal	2065:2074	arg1	performance					2076:2086	high antifungal performance	2060:2086	high antifungal performance at the same time	2060:2103	Supported nanomaterials encapsulated in nanofibers allow formulating active membranes with high antifungal performance at the same time minimizing the risk of nanoparticle release into the environment.
26524992	10	18	from	Copper	1534:1539	arg1	sepiolite					1544:1552	sepiolite	1544:1552	sepiolite	1544:1552	Copper on sepiolite was particularly efficient with a decrease of metabolic activity of up to 80% with respect to controls.
26524992	6	19	theme	fungistatic	1032:1042	arg1	effect					1044:1049	The fungistatic effect	1028:1049	The fungistatic effect	1028:1049	The fungistatic effect took place on germinating spores before hyphae growth conidiophore formation.
26524992	4	20	theme	viability	787:795	arg1	stain					797:801	a viability stain	785:801	a viability stain	785:801	The effect was assessed using digital image analysis to evaluate fungal growth rate and fluorescence readings using a viability stain.
26524992	13	21	theme	total	1947:1951	arg1	load					1953:1956	their total load	1941:1956	their total load of metals	1941:1966	The amount of silver or copper released daily by composite membranes represented roughly 1% of their total load of metals.
26524992	5	22	theme	acetate	999:1005	arg1	membranes					1017:1025	cellulose acetate composite membranes	989:1025	cellulose acetate composite membranes	989:1025	The results showed that silver and copper nanomaterials significantly impaired the growth of fungi when the spores were incubated either in direct contact with particles or included in cellulose acetate composite membranes.
26524992	5	23	with	contact	951:957	arg1	particles					964:972	particles	964:972	particles	964:972	The results showed that silver and copper nanomaterials significantly impaired the growth of fungi when the spores were incubated either in direct contact with particles or included in cellulose acetate composite membranes.
26524992	9	24	theme	copper	1374:1379	arg1	materials					1399:1407	copper and silver loaded materials	1374:1407	copper and silver loaded materials	1374:1407	The effect of copper and silver loaded materials was not significantly different from each other with average reductions around 70% for bare particles and 50% for membranes.
26524992	3	25	theme	larger	546:551	arg1	aggregates					553:562	larger aggregates	546:562	larger aggregates in the micrometer range	546:586	Sepiolite and silica were well dispersed within the fibers, with larger aggregates in the micrometer range, and allowed a controlled release of metals to create a fungistatic environment.
26524992	4	26	theme	fluorescence	757:768	arg1	readings					770:777	fluorescence readings	757:777	fluorescence readings	757:777	The effect was assessed using digital image analysis to evaluate fungal growth rate and fluorescence readings using a viability stain.
26524992	2	27	theme	mesopores	429:437	arg1	size					421:424	the size	417:424	the size of mesopores (5-8nm) in the case of mesoporous silica	417:478	The nanoparticles were in the 3-50nm range for sepiolite supported materials and limited by the size of mesopores (5-8nm) in the case of mesoporous silica.
26524992	0	28	theme	Electrospun	0:10	arg1	composites					30:39	Electrospun cellulose acetate composites	0:39	Electrospun cellulose acetate composites containing	0:50	Electrospun cellulose acetate composites containing supported metal nanoparticles for antifungal membranes.
26524992	9	29	theme	silver	1385:1390	arg1	materials					1399:1407	copper and silver loaded materials	1374:1407	copper and silver loaded materials	1374:1407	The effect of copper and silver loaded materials was not significantly different from each other with average reductions around 70% for bare particles and 50% for membranes.
26524992	1	30	theme	cellulose	120:128	arg1	membranes					277:285	fungistatic membranes	265:285	fungistatic membranes	265:285	Electrospun cellulose acetate composites containing silver and copper nanoparticles supported in sepiolite and mesoporous silica were prepared and tested as fungistatic membranes against the fungus Aspergillus niger.
26524992	1	30	theme	cellulose	120:128	arg1	composites					138:147	Electrospun cellulose acetate composites	108:147	Electrospun cellulose acetate composites containing silver and copper nanoparticles supported in sepiolite and mesoporous silica	108:235	Electrospun cellulose acetate composites containing silver and copper nanoparticles supported in sepiolite and mesoporous silica were prepared and tested as fungistatic membranes against the fungus Aspergillus niger.
26524992	6	31	theme	conidiophore	1105:1116	arg1	formation					1118:1126	hyphae growth conidiophore formation	1091:1126	hyphae growth conidiophore formation	1091:1126	The fungistatic effect took place on germinating spores before hyphae growth conidiophore formation.
26524992	2	32	theme	mesoporous	462:471	arg1	silica					473:478	mesoporous silica	462:478	mesoporous silica	462:478	The nanoparticles were in the 3-50nm range for sepiolite supported materials and limited by the size of mesopores (5-8nm) in the case of mesoporous silica.
26524992	0	33	theme	acetate	22:28	arg1	composites					30:39	Electrospun cellulose acetate composites	0:39	Electrospun cellulose acetate composites containing	0:50	Electrospun cellulose acetate composites containing supported metal nanoparticles for antifungal membranes.
26524992	9	34	theme	materials	1399:1407	arg1	different					1431:1439	different	1431:1439	different	1431:1439	The effect of copper and silver loaded materials was not significantly different from each other with average reductions around 70% for bare particles and 50% for membranes.
26524992	9	34	theme	materials	1399:1407	arg1	effect					1364:1369	The effect	1360:1369	The effect of copper and silver loaded materials	1360:1407	The effect of copper and silver loaded materials was not significantly different from each other with average reductions around 70% for bare particles and 50% for membranes.
26524992	6	35	theme	hyphae	1091:1096	arg1	formation					1118:1126	hyphae growth conidiophore formation	1091:1126	hyphae growth conidiophore formation	1091:1126	The fungistatic effect took place on germinating spores before hyphae growth conidiophore formation.
26524992	14	36	theme	nanoparticle	2128:2139	arg1	release					2141:2147	nanoparticle release	2128:2147	nanoparticle release into the environment	2128:2168	Supported nanomaterials encapsulated in nanofibers allow formulating active membranes with high antifungal performance at the same time minimizing the risk of nanoparticle release into the environment.
26524992	13	37	theme	composite	1895:1903	arg1	membranes					1905:1913	composite membranes	1895:1913	composite membranes	1895:1913	The amount of silver or copper released daily by composite membranes represented roughly 1% of their total load of metals.
26524992	2	38	from	mesopores	429:437	arg1	case					454:457	the case	450:457	the case of mesoporous silica	450:478	The nanoparticles were in the 3-50nm range for sepiolite supported materials and limited by the size of mesopores (5-8nm) in the case of mesoporous silica.
26524992	0	39	theme	metal	62:66	arg1	nanoparticles					68:80	metal nanoparticles	62:80	metal nanoparticles for antifungal membranes	62:105	Electrospun cellulose acetate composites containing supported metal nanoparticles for antifungal membranes.
26524992	2	40	theme	supported	382:390	arg1	materials					392:400	sepiolite supported materials	372:400	sepiolite supported materials	372:400	The nanoparticles were in the 3-50nm range for sepiolite supported materials and limited by the size of mesopores (5-8nm) in the case of mesoporous silica.
26524992	1	41	theme	fungistatic	265:275	arg1	membranes					277:285	fungistatic membranes	265:285	fungistatic membranes	265:285	Electrospun cellulose acetate composites containing silver and copper nanoparticles supported in sepiolite and mesoporous silica were prepared and tested as fungistatic membranes against the fungus Aspergillus niger.
26524992	1	41	theme	fungistatic	265:275	arg1	composites					138:147	Electrospun cellulose acetate composites	108:147	Electrospun cellulose acetate composites containing silver and copper nanoparticles supported in sepiolite and mesoporous silica	108:235	Electrospun cellulose acetate composites containing silver and copper nanoparticles supported in sepiolite and mesoporous silica were prepared and tested as fungistatic membranes against the fungus Aspergillus niger.
26524992	9	42	from	other	1451:1455	arg1	different					1431:1439	different	1431:1439	different	1431:1439	The effect of copper and silver loaded materials was not significantly different from each other with average reductions around 70% for bare particles and 50% for membranes.
26524992	9	42	from	other	1451:1455	arg1	effect					1364:1369	The effect	1360:1369	The effect of copper and silver loaded materials	1360:1407	The effect of copper and silver loaded materials was not significantly different from each other with average reductions around 70% for bare particles and 50% for membranes.
26524992	10	43	theme	metabolic	1600:1608	arg1	activity					1610:1617	metabolic activity	1600:1617	metabolic activity of up to 80% with respect to controls	1600:1655	Copper on sepiolite was particularly efficient with a decrease of metabolic activity of up to 80% with respect to controls.
26524992	11	44	theme	Copper	1658:1663	arg1	materials					1665:1673	Copper materials	1658:1673	Copper materials	1658:1673	Copper materials induced rapid maturation and conidiation with fungi splitting in sets of subcolonies.
26524992	9	45	theme	bare	1496:1499	arg1	particles					1501:1509	bare particles	1496:1509	bare particles	1496:1509	The effect of copper and silver loaded materials was not significantly different from each other with average reductions around 70% for bare particles and 50% for membranes.
26524992	14	46	theme	Supported	1969:1977	arg1	nanomaterials					1979:1991	Supported nanomaterials	1969:1991	Supported nanomaterials encapsulated in nanofibers	1969:2018	Supported nanomaterials encapsulated in nanofibers allow formulating active membranes with high antifungal performance at the same time minimizing the risk of nanoparticle release into the environment.
26524992	2	47	from	case	454:457	arg1	size					421:424	the size	417:424	the size of mesopores (5-8nm) in the case of mesoporous silica	417:478	The nanoparticles were in the 3-50nm range for sepiolite supported materials and limited by the size of mesopores (5-8nm) in the case of mesoporous silica.
26524992	3	48	with	fibers	533:538	arg1	aggregates					553:562	larger aggregates	546:562	larger aggregates in the micrometer range	546:586	Sepiolite and silica were well dispersed within the fibers, with larger aggregates in the micrometer range, and allowed a controlled release of metals to create a fungistatic environment.
26524992	1	49	theme	fungus	299:304	arg1	niger					318:322	the fungus Aspergillus niger	295:322	the fungus Aspergillus niger	295:322	Electrospun cellulose acetate composites containing silver and copper nanoparticles supported in sepiolite and mesoporous silica were prepared and tested as fungistatic membranes against the fungus Aspergillus niger.
26524992	12	50	theme	controlled	1816:1825	arg1	release					1827:1833	the controlled release	1812:1833	the controlled release of metals	1812:1843	Metal-loaded nanomaterials acted as reservoirs for the controlled release of metals.
26524992	1	51	contain	containing	149:158	arg1	membranes					277:285	fungistatic membranes	265:285	fungistatic membranes	265:285	Electrospun cellulose acetate composites containing silver and copper nanoparticles supported in sepiolite and mesoporous silica were prepared and tested as fungistatic membranes against the fungus Aspergillus niger.
26524992	1	51	contain	containing	149:158	arg2	nanoparticles					178:190	silver and copper nanoparticles	160:190	silver and copper nanoparticles supported in sepiolite and mesoporous silica	160:235	Electrospun cellulose acetate composites containing silver and copper nanoparticles supported in sepiolite and mesoporous silica were prepared and tested as fungistatic membranes against the fungus Aspergillus niger.
26524992	1	51	contain	containing	149:158	arg1	composites					138:147	Electrospun cellulose acetate composites	108:147	Electrospun cellulose acetate composites containing silver and copper nanoparticles supported in sepiolite and mesoporous silica	108:235	Electrospun cellulose acetate composites containing silver and copper nanoparticles supported in sepiolite and mesoporous silica were prepared and tested as fungistatic membranes against the fungus Aspergillus niger.
26524992	14	52	theme	high	2060:2063	arg1	performance					2076:2086	high antifungal performance	2060:2086	high antifungal performance at the same time	2060:2103	Supported nanomaterials encapsulated in nanofibers allow formulating active membranes with high antifungal performance at the same time minimizing the risk of nanoparticle release into the environment.
26524992	2	53	from	size	421:424	arg1	case					454:457	the case	450:457	the case of mesoporous silica	450:478	The nanoparticles were in the 3-50nm range for sepiolite supported materials and limited by the size of mesopores (5-8nm) in the case of mesoporous silica.
26524992	10	54	theme	%	1630:1630	arg1	activity					1610:1617	metabolic activity	1600:1617	metabolic activity of up to 80% with respect to controls	1600:1655	Copper on sepiolite was particularly efficient with a decrease of metabolic activity of up to 80% with respect to controls.
26524992	2	55	theme	3-50nm	355:360	arg1	range					362:366	the 3-50nm range	351:366	the 3-50nm range for sepiolite supported materials	351:400	The nanoparticles were in the 3-50nm range for sepiolite supported materials and limited by the size of mesopores (5-8nm) in the case of mesoporous silica.
26524992	8	56	theme	non-exposed	1338:1348	arg1	controls					1350:1357	non-exposed controls	1338:1357	non-exposed controls	1338:1357	Growth reduction was important for all the particles and membranes with respect to non-exposed controls.
26524992	13	57	theme	load	1953:1956	arg1	%					1936:1936	roughly 1%	1927:1936	roughly 1% of their total load of metals	1927:1966	The amount of silver or copper released daily by composite membranes represented roughly 1% of their total load of metals.
26524992	13	57	theme	load	1953:1956	arg1	load					1953:1956	their total load	1941:1956	their total load of metals	1941:1966	The amount of silver or copper released daily by composite membranes represented roughly 1% of their total load of metals.
26524992	6	58	theme	germinating	1065:1075	arg1	spores					1077:1082	germinating spores	1065:1082	germinating spores	1065:1082	The fungistatic effect took place on germinating spores before hyphae growth conidiophore formation.
26524992	14	59	theme	same	2095:2098	arg1	time					2100:2103	the same time	2091:2103	the same time	2091:2103	Supported nanomaterials encapsulated in nanofibers allow formulating active membranes with high antifungal performance at the same time minimizing the risk of nanoparticle release into the environment.
26524992	4	60	theme	growth	741:746	arg1	rate					748:751	fungal growth rate	734:751	fungal growth rate	734:751	The effect was assessed using digital image analysis to evaluate fungal growth rate and fluorescence readings using a viability stain.
26524992	13	61	theme	metals	1961:1966	arg1	load					1953:1956	their total load	1941:1956	their total load of metals	1941:1966	The amount of silver or copper released daily by composite membranes represented roughly 1% of their total load of metals.
26524992	0	62	theme	antifungal	86:95	arg1	membranes					97:105	antifungal membranes	86:105	antifungal membranes	86:105	Electrospun cellulose acetate composites containing supported metal nanoparticles for antifungal membranes.
26524992	3	63	theme	metals	625:630	arg1	release					614:620	a controlled release	601:620	a controlled release of metals	601:630	Sepiolite and silica were well dispersed within the fibers, with larger aggregates in the micrometer range, and allowed a controlled release of metals to create a fungistatic environment.
26524992	12	64	theme	Metal-loaded	1761:1772	arg1	reservoirs					1797:1806	reservoirs	1797:1806	reservoirs for the controlled release of metals	1797:1843	Metal-loaded nanomaterials acted as reservoirs for the controlled release of metals.
26524992	12	64	theme	Metal-loaded	1761:1772	arg1	nanomaterials					1774:1786	Metal-loaded nanomaterials	1761:1786	Metal-loaded nanomaterials	1761:1786	Metal-loaded nanomaterials acted as reservoirs for the controlled release of metals.
26524992	4	65	theme	digital	699:705	arg1	analysis					713:720	digital image analysis	699:720	digital image analysis	699:720	The effect was assessed using digital image analysis to evaluate fungal growth rate and fluorescence readings using a viability stain.
26524992	3	66	from	aggregates	553:562	arg1	range					582:586	the micrometer range	567:586	the micrometer range	567:586	Sepiolite and silica were well dispersed within the fibers, with larger aggregates in the micrometer range, and allowed a controlled release of metals to create a fungistatic environment.
26524992	5	67	theme	cellulose	989:997	arg1	membranes					1017:1025	cellulose acetate composite membranes	989:1025	cellulose acetate composite membranes	989:1025	The results showed that silver and copper nanomaterials significantly impaired the growth of fungi when the spores were incubated either in direct contact with particles or included in cellulose acetate composite membranes.
26524992	10	68	with	efficient	1571:1579	arg1	decrease					1588:1595	a decrease	1586:1595	a decrease of metabolic activity of up to 80% with respect to controls	1586:1655	Copper on sepiolite was particularly efficient with a decrease of metabolic activity of up to 80% with respect to controls.
26524992	7	69	theme	prior	1239:1243	arg1	exposure					1245:1252	the prior exposure	1235:1252	the prior exposure	1235:1252	After 24h the cultures were separated from fungistatic materials and showed growth impairment only due to the prior exposure.
26524992	5	70	theme	composite	1007:1015	arg1	membranes					1017:1025	cellulose acetate composite membranes	989:1025	cellulose acetate composite membranes	989:1025	The results showed that silver and copper nanomaterials significantly impaired the growth of fungi when the spores were incubated either in direct contact with particles or included in cellulose acetate composite membranes.
26524992	5	71	theme	silver	828:833	arg1	nanomaterials					846:858	silver and copper nanomaterials	828:858	silver and copper nanomaterials	828:858	The results showed that silver and copper nanomaterials significantly impaired the growth of fungi when the spores were incubated either in direct contact with particles or included in cellulose acetate composite membranes.
26524992	1	72	theme	Electrospun	108:118	arg1	membranes					277:285	fungistatic membranes	265:285	fungistatic membranes	265:285	Electrospun cellulose acetate composites containing silver and copper nanoparticles supported in sepiolite and mesoporous silica were prepared and tested as fungistatic membranes against the fungus Aspergillus niger.
26524992	1	72	theme	Electrospun	108:118	arg1	composites					138:147	Electrospun cellulose acetate composites	108:147	Electrospun cellulose acetate composites containing silver and copper nanoparticles supported in sepiolite and mesoporous silica	108:235	Electrospun cellulose acetate composites containing silver and copper nanoparticles supported in sepiolite and mesoporous silica were prepared and tested as fungistatic membranes against the fungus Aspergillus niger.
26524992	2	73	theme	silica	473:478	arg1	case					454:457	the case	450:457	the case of mesoporous silica	450:478	The nanoparticles were in the 3-50nm range for sepiolite supported materials and limited by the size of mesopores (5-8nm) in the case of mesoporous silica.
26524992	5	74	dep	either	934:939	arg1	contact					951:957	direct contact	944:957	direct contact with particles	944:972	The results showed that silver and copper nanomaterials significantly impaired the growth of fungi when the spores were incubated either in direct contact with particles or included in cellulose acetate composite membranes.
26524992	5	75	theme	copper	839:844	arg1	nanomaterials					846:858	silver and copper nanomaterials	828:858	silver and copper nanomaterials	828:858	The results showed that silver and copper nanomaterials significantly impaired the growth of fungi when the spores were incubated either in direct contact with particles or included in cellulose acetate composite membranes.
26524992	9	76	theme	loaded	1392:1397	arg1	materials					1399:1407	copper and silver loaded materials	1374:1407	copper and silver loaded materials	1374:1407	The effect of copper and silver loaded materials was not significantly different from each other with average reductions around 70% for bare particles and 50% for membranes.
26524992	7	77	theme	fungistatic	1172:1182	arg1	materials					1184:1192	fungistatic materials	1172:1192	fungistatic materials	1172:1192	After 24h the cultures were separated from fungistatic materials and showed growth impairment only due to the prior exposure.
26524992	1	78	theme	acetate	130:136	arg1	membranes					277:285	fungistatic membranes	265:285	fungistatic membranes	265:285	Electrospun cellulose acetate composites containing silver and copper nanoparticles supported in sepiolite and mesoporous silica were prepared and tested as fungistatic membranes against the fungus Aspergillus niger.
26524992	1	78	theme	acetate	130:136	arg1	composites					138:147	Electrospun cellulose acetate composites	108:147	Electrospun cellulose acetate composites containing silver and copper nanoparticles supported in sepiolite and mesoporous silica	108:235	Electrospun cellulose acetate composites containing silver and copper nanoparticles supported in sepiolite and mesoporous silica were prepared and tested as fungistatic membranes against the fungus Aspergillus niger.
26524992	6	79	theme	growth	1098:1103	arg1	formation					1118:1126	hyphae growth conidiophore formation	1091:1126	hyphae growth conidiophore formation	1091:1126	The fungistatic effect took place on germinating spores before hyphae growth conidiophore formation.
26524992	14	80	theme	release	2141:2147	arg1	risk					2120:2123	the risk	2116:2123	the risk of nanoparticle release into the environment	2116:2168	Supported nanomaterials encapsulated in nanofibers allow formulating active membranes with high antifungal performance at the same time minimizing the risk of nanoparticle release into the environment.
26524992	7	81	theme	growth	1205:1210	arg1	impairment					1212:1221	growth impairment	1205:1221	growth impairment	1205:1221	After 24h the cultures were separated from fungistatic materials and showed growth impairment only due to the prior exposure.
26524992	14	82	theme	active	2038:2043	arg1	membranes					2045:2053	active membranes	2038:2053	active membranes	2038:2053	Supported nanomaterials encapsulated in nanofibers allow formulating active membranes with high antifungal performance at the same time minimizing the risk of nanoparticle release into the environment.
26524992	3	83	theme	fungistatic	644:654	arg1	environment					656:666	a fungistatic environment	642:666	a fungistatic environment	642:666	Sepiolite and silica were well dispersed within the fibers, with larger aggregates in the micrometer range, and allowed a controlled release of metals to create a fungistatic environment.
26524992	14	84	from	time	2100:2103	arg1	performance					2076:2086	high antifungal performance	2060:2086	high antifungal performance at the same time	2060:2103	Supported nanomaterials encapsulated in nanofibers allow formulating active membranes with high antifungal performance at the same time minimizing the risk of nanoparticle release into the environment.
26524992	11	85	theme	subcolonies	1748:1758	arg1	sets					1740:1743	sets	1740:1743	sets of subcolonies	1740:1758	Copper materials induced rapid maturation and conidiation with fungi splitting in sets of subcolonies.
26524992	11	85	theme	subcolonies	1748:1758	arg1	subcolonies					1748:1758	subcolonies	1748:1758	subcolonies	1748:1758	Copper materials induced rapid maturation and conidiation with fungi splitting in sets of subcolonies.
26524992	2	86	theme	sepiolite	372:380	arg1	materials					392:400	sepiolite supported materials	372:400	sepiolite supported materials	372:400	The nanoparticles were in the 3-50nm range for sepiolite supported materials and limited by the size of mesopores (5-8nm) in the case of mesoporous silica.
26524992	12	87	theme	metals	1838:1843	arg1	release					1827:1833	the controlled release	1812:1833	the controlled release of metals	1812:1843	Metal-loaded nanomaterials acted as reservoirs for the controlled release of metals.
26524992	13	88	theme	silver	1860:1865	arg1	silver					1860:1865	silver	1860:1865	silver	1860:1865	The amount of silver or copper released daily by composite membranes represented roughly 1% of their total load of metals.
26524992	13	88	theme	silver	1860:1865	arg1	amount					1850:1855	The amount	1846:1855	The amount of silver or copper released daily by composite membranes	1846:1913	The amount of silver or copper released daily by composite membranes represented roughly 1% of their total load of metals.
26524992	13	88	theme	silver	1860:1865	arg1	copper					1870:1875	copper	1870:1875	copper	1870:1875	The amount of silver or copper released daily by composite membranes represented roughly 1% of their total load of metals.
26524992	11	89	theme	rapid	1683:1687	arg1	maturation					1689:1698	maturation	1689:1698	maturation	1689:1698	Copper materials induced rapid maturation and conidiation with fungi splitting in sets of subcolonies.
26524992	0	90	theme	cellulose	12:20	arg1	composites					30:39	Electrospun cellulose acetate composites	0:39	Electrospun cellulose acetate composites containing	0:50	Electrospun cellulose acetate composites containing supported metal nanoparticles for antifungal membranes.
26524992	9	91	theme	average	1462:1468	arg1	reductions					1470:1479	average reductions	1462:1479	average reductions around 70% for bare particles and 50% for membranes	1462:1531	The effect of copper and silver loaded materials was not significantly different from each other with average reductions around 70% for bare particles and 50% for membranes.
25582492	8	0	theme	saccharide	1610:1619	arg1	polymer					1621:1627	the crosslinked saccharide polymer	1594:1627	the crosslinked saccharide polymer	1594:1627	Thus, the ee of 1E was low in solution and suspension, but suddenly leaped at the phase border of flowing gel and rigid gel to give the highest ee of 22–24%, which are much higher than those obtained with CD-NSs (6–12% ee), revealing the positive roles of the chiral void space formed upon gelation of the crosslinked saccharide polymer.
25582492	2	1	theme	different	398:406	arg1	degrees					408:414	different degrees	398:414	different degrees	398:414	Cyclic nigerosylnigerose (CNN), a saucer-shaped cyclic tetrasaccharide with a shallow concave surface, was reacted with pyromellitic dianhydride in 1:2 and 1:4 ratios to give two CNN-based polymers of different degrees of crosslinking, both of which swelled upon soaking in water, acting as a ‘nanosponge’ (NS).
25582492	8	2	from	suspension	1335:1344	arg1	low					1315:1317	low	1315:1317	low	1315:1317	Thus, the ee of 1E was low in solution and suspension, but suddenly leaped at the phase border of flowing gel and rigid gel to give the highest ee of 22–24%, which are much higher than those obtained with CD-NSs (6–12% ee), revealing the positive roles of the chiral void space formed upon gelation of the crosslinked saccharide polymer.
25582492	8	3	theme	gel	1398:1400	arg1	border					1380:1385	the phase border	1370:1385	the phase border of flowing gel and rigid gel	1370:1414	Thus, the ee of 1E was low in solution and suspension, but suddenly leaped at the phase border of flowing gel and rigid gel to give the highest ee of 22–24%, which are much higher than those obtained with CD-NSs (6–12% ee), revealing the positive roles of the chiral void space formed upon gelation of the crosslinked saccharide polymer.
25582492	8	4	theme	phase	1374:1378	arg1	border					1380:1385	the phase border	1370:1385	the phase border of flowing gel and rigid gel	1370:1414	Thus, the ee of 1E was low in solution and suspension, but suddenly leaped at the phase border of flowing gel and rigid gel to give the highest ee of 22–24%, which are much higher than those obtained with CD-NSs (6–12% ee), revealing the positive roles of the chiral void space formed upon gelation of the crosslinked saccharide polymer.
25582492	2	5	with	tetrasaccharide	252:266	arg1	surface					291:297	a shallow concave surface	273:297	a shallow concave surface	273:297	Cyclic nigerosylnigerose (CNN), a saucer-shaped cyclic tetrasaccharide with a shallow concave surface, was reacted with pyromellitic dianhydride in 1:2 and 1:4 ratios to give two CNN-based polymers of different degrees of crosslinking, both of which swelled upon soaking in water, acting as a ‘nanosponge’ (NS).
25582492	3	6	from	solution	557:564	arg1	phases					535:540	several phases	527:540	several phases from isotropic solution to flowing and rigid gels	527:590	These NSs evolved several phases from isotropic solution to flowing and rigid gels via suspension by gradually increasing the concentration in water.
25582492	6	7	theme	isotropic	968:976	arg1	solution					978:985	isotropic solution	968:985	isotropic solution	968:985	In isotropic solution, the enantioselectivity was generally low (−4% to +6% ee) but the chiral sense of 1E was inverted by increasing the methanol content.
25582492	8	8	theme	flowing	1390:1396	arg1	gel					1398:1400	flowing gel and rigid gel	1390:1414	gel	1398:1400	Thus, the ee of 1E was low in solution and suspension, but suddenly leaped at the phase border of flowing gel and rigid gel to give the highest ee of 22–24%, which are much higher than those obtained with CD-NSs (6–12% ee), revealing the positive roles of the chiral void space formed upon gelation of the crosslinked saccharide polymer.
25582492	2	9	dep	1:2	345:347	arg1	give					367:370	give	367:370	to give two CNN-based polymers of different degrees of crosslinking, both of which swelled upon soaking in water, acting as a ‘nanosponge’ (NS)	364:506	Cyclic nigerosylnigerose (CNN), a saucer-shaped cyclic tetrasaccharide with a shallow concave surface, was reacted with pyromellitic dianhydride in 1:2 and 1:4 ratios to give two CNN-based polymers of different degrees of crosslinking, both of which swelled upon soaking in water, acting as a ‘nanosponge’ (NS).
25582492	5	10	theme	different	923:931	arg1	concentrations					940:953	different CNN-NS concentrations	923:953	different CNN-NS concentrations in water	923:962	The enantiomeric excess (ee) of 1E obtained was a critical function of the solvent composition and the phase evolved at different CNN-NS concentrations in water.
25582492	6	11	dep	low	1025:1027	arg1	ee					1041:1042	−4% to +6% ee	1030:1042	−4% to +6% ee	1030:1042	In isotropic solution, the enantioselectivity was generally low (−4% to +6% ee) but the chiral sense of 1E was inverted by increasing the methanol content.
25582492	6	11	dep	low	1025:1027	arg1	enantioselectivity					992:1009	the enantioselectivity	988:1009	the enantioselectivity	988:1009	In isotropic solution, the enantioselectivity was generally low (−4% to +6% ee) but the chiral sense of 1E was inverted by increasing the methanol content.
25582492	6	11	dep	low	1025:1027	arg1	low					1025:1027	low	1025:1027	low	1025:1027	In isotropic solution, the enantioselectivity was generally low (−4% to +6% ee) but the chiral sense of 1E was inverted by increasing the methanol content.
25582492	8	12	theme	rigid	1406:1410	arg1	gel					1412:1414	flowing gel and rigid gel	1390:1414	gel	1412:1414	Thus, the ee of 1E was low in solution and suspension, but suddenly leaped at the phase border of flowing gel and rigid gel to give the highest ee of 22–24%, which are much higher than those obtained with CD-NSs (6–12% ee), revealing the positive roles of the chiral void space formed upon gelation of the crosslinked saccharide polymer.
25582492	3	13	theme	flowing	569:575	arg1	gels					587:590	flowing and rigid gels	569:590	flowing and rigid gels	569:590	These NSs evolved several phases from isotropic solution to flowing and rigid gels via suspension by gradually increasing the concentration in water.
25582492	1	14	theme	Z	95:95	arg1	-cyclooctene					97:108	(Z)-cyclooctene	94:108	(Z)-cyclooctene	94:108	Enantiodifferentiating photoisomerization of (Z)-cyclooctene sensitized by cyclic nigerosylnigerose-based nanosponges crosslinked by pyromellitate.
25582492	8	15	theme	positive	1530:1537	arg1	roles					1539:1543	the positive roles	1526:1543	the positive roles of the chiral void space formed upon gelation of the crosslinked saccharide polymer	1526:1627	Thus, the ee of 1E was low in solution and suspension, but suddenly leaped at the phase border of flowing gel and rigid gel to give the highest ee of 22–24%, which are much higher than those obtained with CD-NSs (6–12% ee), revealing the positive roles of the chiral void space formed upon gelation of the crosslinked saccharide polymer.
25582492	6	16	dep	%	1039:1039	arg1	to					1034:1035	to	1034:1035	to	1034:1035	In isotropic solution, the enantioselectivity was generally low (−4% to +6% ee) but the chiral sense of 1E was inverted by increasing the methanol content.
25582492	6	16	dep	%	1039:1039	arg1	%					1032:1032	%	1032:1032	%	1032:1032	In isotropic solution, the enantioselectivity was generally low (−4% to +6% ee) but the chiral sense of 1E was inverted by increasing the methanol content.
25582492	8	17	link	crosslinked	1598:1608	arg1	polymer					1621:1627	the crosslinked saccharide polymer	1594:1627	the crosslinked saccharide polymer	1594:1627	Thus, the ee of 1E was low in solution and suspension, but suddenly leaped at the phase border of flowing gel and rigid gel to give the highest ee of 22–24%, which are much higher than those obtained with CD-NSs (6–12% ee), revealing the positive roles of the chiral void space formed upon gelation of the crosslinked saccharide polymer.
25582492	5	18	theme	solvent	878:884	arg1	composition					886:896	the solvent composition	874:896	the solvent composition	874:896	The enantiomeric excess (ee) of 1E obtained was a critical function of the solvent composition and the phase evolved at different CNN-NS concentrations in water.
25582492	6	19	theme	methanol	1103:1110	arg1	content					1112:1118	the methanol content	1099:1118	the methanol content	1099:1118	In isotropic solution, the enantioselectivity was generally low (−4% to +6% ee) but the chiral sense of 1E was inverted by increasing the methanol content.
25582492	8	20	theme	%	1509:1509	arg1	CD-NSs					1497:1502	CD-NSs	1497:1502	CD-NSs (6–12% ee)	1497:1513	Thus, the ee of 1E was low in solution and suspension, but suddenly leaped at the phase border of flowing gel and rigid gel to give the highest ee of 22–24%, which are much higher than those obtained with CD-NSs (6–12% ee), revealing the positive roles of the chiral void space formed upon gelation of the crosslinked saccharide polymer.
25582492	8	20	theme	%	1509:1509	arg1	ee					1511:1512	6–12% ee	1505:1512	6–12% ee	1505:1512	Thus, the ee of 1E was low in solution and suspension, but suddenly leaped at the phase border of flowing gel and rigid gel to give the highest ee of 22–24%, which are much higher than those obtained with CD-NSs (6–12% ee), revealing the positive roles of the chiral void space formed upon gelation of the crosslinked saccharide polymer.
25582492	5	21	theme	1E	835:836	arg1	function					862:869	a critical function	851:869	a critical function of the solvent composition	851:896	The enantiomeric excess (ee) of 1E obtained was a critical function of the solvent composition and the phase evolved at different CNN-NS concentrations in water.
25582492	5	21	theme	1E	835:836	arg1	ee					828:829	ee	828:829	ee	828:829	The enantiomeric excess (ee) of 1E obtained was a critical function of the solvent composition and the phase evolved at different CNN-NS concentrations in water.
25582492	5	21	theme	1E	835:836	arg1	excess					820:825	The enantiomeric excess	803:825	The enantiomeric excess (ee) of 1E obtained	803:845	The enantiomeric excess (ee) of 1E obtained was a critical function of the solvent composition and the phase evolved at different CNN-NS concentrations in water.
25582492	2	22	theme	Cyclic	197:202	arg1	tetrasaccharide					252:266	a saucer-shaped cyclic tetrasaccharide	229:266	a saucer-shaped cyclic tetrasaccharide with a shallow concave surface	229:297	Cyclic nigerosylnigerose (CNN), a saucer-shaped cyclic tetrasaccharide with a shallow concave surface, was reacted with pyromellitic dianhydride in 1:2 and 1:4 ratios to give two CNN-based polymers of different degrees of crosslinking, both of which swelled upon soaking in water, acting as a ‘nanosponge’ (NS).
25582492	2	22	theme	Cyclic	197:202	arg1	CNN					223:225	CNN	223:225	CNN	223:225	Cyclic nigerosylnigerose (CNN), a saucer-shaped cyclic tetrasaccharide with a shallow concave surface, was reacted with pyromellitic dianhydride in 1:2 and 1:4 ratios to give two CNN-based polymers of different degrees of crosslinking, both of which swelled upon soaking in water, acting as a ‘nanosponge’ (NS).
25582492	2	22	theme	Cyclic	197:202	arg1	nigerosylnigerose					204:220	Cyclic nigerosylnigerose	197:220	Cyclic nigerosylnigerose (CNN)	197:226	Cyclic nigerosylnigerose (CNN), a saucer-shaped cyclic tetrasaccharide with a shallow concave surface, was reacted with pyromellitic dianhydride in 1:2 and 1:4 ratios to give two CNN-based polymers of different degrees of crosslinking, both of which swelled upon soaking in water, acting as a ‘nanosponge’ (NS).
25582492	6	23	from	low	1025:1027	arg1	solution					978:985	isotropic solution	968:985	isotropic solution	968:985	In isotropic solution, the enantioselectivity was generally low (−4% to +6% ee) but the chiral sense of 1E was inverted by increasing the methanol content.
25582492	1	24	theme	-cyclooctene	97:108	arg1	photoisomerization					72:89	Enantiodifferentiating photoisomerization	49:89	Enantiodifferentiating photoisomerization of (Z)-cyclooctene	49:108	Enantiodifferentiating photoisomerization of (Z)-cyclooctene sensitized by cyclic nigerosylnigerose-based nanosponges crosslinked by pyromellitate.
25582492	3	25	from	concentration	635:647	arg1	water					652:656	water	652:656	water	652:656	These NSs evolved several phases from isotropic solution to flowing and rigid gels via suspension by gradually increasing the concentration in water.
25582492	6	26	theme	+6	1037:1038	arg1	%					1039:1039	%	1039:1039	%	1039:1039	In isotropic solution, the enantioselectivity was generally low (−4% to +6% ee) but the chiral sense of 1E was inverted by increasing the methanol content.
25582492	8	27	theme	gel	1412:1414	arg1	border					1380:1385	the phase border	1370:1385	the phase border of flowing gel and rigid gel	1370:1414	Thus, the ee of 1E was low in solution and suspension, but suddenly leaped at the phase border of flowing gel and rigid gel to give the highest ee of 22–24%, which are much higher than those obtained with CD-NSs (6–12% ee), revealing the positive roles of the chiral void space formed upon gelation of the crosslinked saccharide polymer.
25582492	6	28	from	solution	978:985	arg1	ee					1041:1042	−4% to +6% ee	1030:1042	−4% to +6% ee	1030:1042	In isotropic solution, the enantioselectivity was generally low (−4% to +6% ee) but the chiral sense of 1E was inverted by increasing the methanol content.
25582492	6	28	from	solution	978:985	arg1	enantioselectivity					992:1009	the enantioselectivity	988:1009	the enantioselectivity	988:1009	In isotropic solution, the enantioselectivity was generally low (−4% to +6% ee) but the chiral sense of 1E was inverted by increasing the methanol content.
25582492	6	28	from	solution	978:985	arg1	low					1025:1027	low	1025:1027	low	1025:1027	In isotropic solution, the enantioselectivity was generally low (−4% to +6% ee) but the chiral sense of 1E was inverted by increasing the methanol content.
25582492	3	29	theme	rigid	581:585	arg1	gels					587:590	flowing and rigid gels	569:590	flowing and rigid gels	569:590	These NSs evolved several phases from isotropic solution to flowing and rigid gels via suspension by gradually increasing the concentration in water.
25582492	0	30	theme	Solvent-	0:7	arg1	photochirogenesis					30:46	Solvent- and phase-controlled photochirogenesis	0:46	Solvent- and phase-controlled photochirogenesis.	0:47	Solvent- and phase-controlled photochirogenesis.
25582492	8	31	theme	chiral	1552:1557	arg1	space					1564:1568	the chiral void space	1548:1568	the chiral void space formed upon gelation of the crosslinked saccharide polymer	1548:1627	Thus, the ee of 1E was low in solution and suspension, but suddenly leaped at the phase border of flowing gel and rigid gel to give the highest ee of 22–24%, which are much higher than those obtained with CD-NSs (6–12% ee), revealing the positive roles of the chiral void space formed upon gelation of the crosslinked saccharide polymer.
25582492	2	32	theme	crosslinking	419:430	arg1	degrees					408:414	different degrees	398:414	different degrees	398:414	Cyclic nigerosylnigerose (CNN), a saucer-shaped cyclic tetrasaccharide with a shallow concave surface, was reacted with pyromellitic dianhydride in 1:2 and 1:4 ratios to give two CNN-based polymers of different degrees of crosslinking, both of which swelled upon soaking in water, acting as a ‘nanosponge’ (NS).
25582492	5	33	theme	enantiomeric	807:818	arg1	function					862:869	a critical function	851:869	a critical function of the solvent composition	851:896	The enantiomeric excess (ee) of 1E obtained was a critical function of the solvent composition and the phase evolved at different CNN-NS concentrations in water.
25582492	5	33	theme	enantiomeric	807:818	arg1	ee					828:829	ee	828:829	ee	828:829	The enantiomeric excess (ee) of 1E obtained was a critical function of the solvent composition and the phase evolved at different CNN-NS concentrations in water.
25582492	5	33	theme	enantiomeric	807:818	arg1	excess					820:825	The enantiomeric excess	803:825	The enantiomeric excess (ee) of 1E obtained	803:845	The enantiomeric excess (ee) of 1E obtained was a critical function of the solvent composition and the phase evolved at different CNN-NS concentrations in water.
25582492	7	34	dep	case	1219:1222	arg1	was					1211:1213	was	1211:1213	was	1211:1213	Interestingly, the product's ee was controlled more dramatically by the phase evolved, as was the case with the cyclodextrin-based nanosponge (CD-NS) reported previously.
25582492	7	34	dep	case	1219:1222	arg1	as					1208:1209	as	1208:1209	as	1208:1209	Interestingly, the product's ee was controlled more dramatically by the phase evolved, as was the case with the cyclodextrin-based nanosponge (CD-NS) reported previously.
25582492	2	35	theme	1:4	353:355	arg1	ratios					357:362	1:4 ratios	353:362	1:4 ratios	353:362	Cyclic nigerosylnigerose (CNN), a saucer-shaped cyclic tetrasaccharide with a shallow concave surface, was reacted with pyromellitic dianhydride in 1:2 and 1:4 ratios to give two CNN-based polymers of different degrees of crosslinking, both of which swelled upon soaking in water, acting as a ‘nanosponge’ (NS).
25582492	8	36	from	solution	1322:1329	arg1	low					1315:1317	low	1315:1317	low	1315:1317	Thus, the ee of 1E was low in solution and suspension, but suddenly leaped at the phase border of flowing gel and rigid gel to give the highest ee of 22–24%, which are much higher than those obtained with CD-NSs (6–12% ee), revealing the positive roles of the chiral void space formed upon gelation of the crosslinked saccharide polymer.
25582492	5	37	theme	CNN-NS	933:938	arg1	concentrations					940:953	different CNN-NS concentrations	923:953	different CNN-NS concentrations in water	923:962	The enantiomeric excess (ee) of 1E obtained was a critical function of the solvent composition and the phase evolved at different CNN-NS concentrations in water.
25582492	0	38	theme	phase-controlled	13:28	arg1	photochirogenesis					30:46	Solvent- and phase-controlled photochirogenesis	0:46	Solvent- and phase-controlled photochirogenesis.	0:47	Solvent- and phase-controlled photochirogenesis.
25582492	1	39	theme	nigerosylnigerose-based	131:153	arg1	nanosponges					155:165	cyclic nigerosylnigerose-based nanosponges	124:165	cyclic nigerosylnigerose-based nanosponges crosslinked by pyromellitate	124:194	Enantiodifferentiating photoisomerization of (Z)-cyclooctene sensitized by cyclic nigerosylnigerose-based nanosponges crosslinked by pyromellitate.
25582492	8	40	theme	space	1564:1568	arg1	roles					1539:1543	the positive roles	1526:1543	the positive roles of the chiral void space formed upon gelation of the crosslinked saccharide polymer	1526:1627	Thus, the ee of 1E was low in solution and suspension, but suddenly leaped at the phase border of flowing gel and rigid gel to give the highest ee of 22–24%, which are much higher than those obtained with CD-NSs (6–12% ee), revealing the positive roles of the chiral void space formed upon gelation of the crosslinked saccharide polymer.
25582492	4	41	theme	enantiodifferentiating	710:731	arg1	photoisomerization					733:750	the enantiodifferentiating photoisomerization	706:750	the enantiodifferentiating photoisomerization of (Z)-cyclooctene (1Z) to chiral (E)-isomer (1E)	706:800	The CNN-NSs thus prepared effectively mediated the enantiodifferentiating photoisomerization of (Z)-cyclooctene (1Z) to chiral (E)-isomer (1E).
25582492	5	42	theme	composition	886:896	arg1	function					862:869	a critical function	851:869	a critical function of the solvent composition	851:896	The enantiomeric excess (ee) of 1E obtained was a critical function of the solvent composition and the phase evolved at different CNN-NS concentrations in water.
25582492	5	42	theme	composition	886:896	arg1	excess					820:825	The enantiomeric excess	803:825	The enantiomeric excess (ee) of 1E obtained	803:845	The enantiomeric excess (ee) of 1E obtained was a critical function of the solvent composition and the phase evolved at different CNN-NS concentrations in water.
25582492	5	43	from	concentrations	940:953	arg1	water					958:962	water	958:962	water	958:962	The enantiomeric excess (ee) of 1E obtained was a critical function of the solvent composition and the phase evolved at different CNN-NS concentrations in water.
25582492	3	44	theme	isotropic	547:555	arg1	solution					557:564	isotropic solution	547:564	isotropic solution	547:564	These NSs evolved several phases from isotropic solution to flowing and rigid gels via suspension by gradually increasing the concentration in water.
25582492	8	45	theme	void	1559:1562	arg1	space					1564:1568	the chiral void space	1548:1568	the chiral void space formed upon gelation of the crosslinked saccharide polymer	1548:1627	Thus, the ee of 1E was low in solution and suspension, but suddenly leaped at the phase border of flowing gel and rigid gel to give the highest ee of 22–24%, which are much higher than those obtained with CD-NSs (6–12% ee), revealing the positive roles of the chiral void space formed upon gelation of the crosslinked saccharide polymer.
25582492	7	46	theme	cyclodextrin-based	1233:1250	arg1	CD-NS					1264:1268	the cyclodextrin-based nanosponge (CD-NS)	1229:1269	the cyclodextrin-based nanosponge (CD-NS) reported previously	1229:1289	Interestingly, the product's ee was controlled more dramatically by the phase evolved, as was the case with the cyclodextrin-based nanosponge (CD-NS) reported previously.
25582492	7	47	theme	nanosponge	1252:1261	arg1	CD-NS					1264:1268	the cyclodextrin-based nanosponge (CD-NS)	1229:1269	the cyclodextrin-based nanosponge (CD-NS) reported previously	1229:1289	Interestingly, the product's ee was controlled more dramatically by the phase evolved, as was the case with the cyclodextrin-based nanosponge (CD-NS) reported previously.
25582492	4	48	theme	chiral	779:784	arg1	1E					798:799	1E	798:799	1E	798:799	The CNN-NSs thus prepared effectively mediated the enantiodifferentiating photoisomerization of (Z)-cyclooctene (1Z) to chiral (E)-isomer (1E).
25582492	4	48	theme	chiral	779:784	arg1	-isomer					789:795	chiral (E)-isomer	779:795	chiral (E)-isomer (1E)	779:800	The CNN-NSs thus prepared effectively mediated the enantiodifferentiating photoisomerization of (Z)-cyclooctene (1Z) to chiral (E)-isomer (1E).
25582492	4	48	theme	chiral	779:784	arg1	E					787:787	E	787:787	E	787:787	The CNN-NSs thus prepared effectively mediated the enantiodifferentiating photoisomerization of (Z)-cyclooctene (1Z) to chiral (E)-isomer (1E).
25582492	2	49	theme	concave	283:289	arg1	surface					291:297	a shallow concave surface	273:297	a shallow concave surface	273:297	Cyclic nigerosylnigerose (CNN), a saucer-shaped cyclic tetrasaccharide with a shallow concave surface, was reacted with pyromellitic dianhydride in 1:2 and 1:4 ratios to give two CNN-based polymers of different degrees of crosslinking, both of which swelled upon soaking in water, acting as a ‘nanosponge’ (NS).
25582492	5	50	theme	critical	853:860	arg1	function					862:869	a critical function	851:869	a critical function of the solvent composition	851:896	The enantiomeric excess (ee) of 1E obtained was a critical function of the solvent composition and the phase evolved at different CNN-NS concentrations in water.
25582492	5	50	theme	critical	853:860	arg1	excess					820:825	The enantiomeric excess	803:825	The enantiomeric excess (ee) of 1E obtained	803:845	The enantiomeric excess (ee) of 1E obtained was a critical function of the solvent composition and the phase evolved at different CNN-NS concentrations in water.
25582492	2	51	theme	shallow	275:281	arg1	surface					291:297	a shallow concave surface	273:297	a shallow concave surface	273:297	Cyclic nigerosylnigerose (CNN), a saucer-shaped cyclic tetrasaccharide with a shallow concave surface, was reacted with pyromellitic dianhydride in 1:2 and 1:4 ratios to give two CNN-based polymers of different degrees of crosslinking, both of which swelled upon soaking in water, acting as a ‘nanosponge’ (NS).
25582492	4	52	theme	Z	756:756	arg1	1Z					772:773	1Z	772:773	1Z	772:773	The CNN-NSs thus prepared effectively mediated the enantiodifferentiating photoisomerization of (Z)-cyclooctene (1Z) to chiral (E)-isomer (1E).
25582492	4	52	theme	Z	756:756	arg1	-cyclooctene					758:769	(Z)-cyclooctene	755:769	(Z)-cyclooctene (1Z)	755:774	The CNN-NSs thus prepared effectively mediated the enantiodifferentiating photoisomerization of (Z)-cyclooctene (1Z) to chiral (E)-isomer (1E).
25582492	8	53	from	low	1315:1317	arg1	suspension					1335:1344	suspension	1335:1344	suspension	1335:1344	Thus, the ee of 1E was low in solution and suspension, but suddenly leaped at the phase border of flowing gel and rigid gel to give the highest ee of 22–24%, which are much higher than those obtained with CD-NSs (6–12% ee), revealing the positive roles of the chiral void space formed upon gelation of the crosslinked saccharide polymer.
25582492	8	53	from	low	1315:1317	arg1	solution					1322:1329	solution	1322:1329	solution	1322:1329	Thus, the ee of 1E was low in solution and suspension, but suddenly leaped at the phase border of flowing gel and rigid gel to give the highest ee of 22–24%, which are much higher than those obtained with CD-NSs (6–12% ee), revealing the positive roles of the chiral void space formed upon gelation of the crosslinked saccharide polymer.
25582492	8	54	theme	highest	1428:1434	arg1	ee					1436:1437	the highest ee	1424:1437	the highest ee of 22–24%, which are much higher than those obtained with CD-NSs (6–12% ee), revealing the positive roles of the chiral void space formed upon gelation of the crosslinked saccharide polymer	1424:1627	Thus, the ee of 1E was low in solution and suspension, but suddenly leaped at the phase border of flowing gel and rigid gel to give the highest ee of 22–24%, which are much higher than those obtained with CD-NSs (6–12% ee), revealing the positive roles of the chiral void space formed upon gelation of the crosslinked saccharide polymer.
25582492	8	55	theme	1E	1308:1309	arg1	ee					1302:1303	the ee	1298:1303	the ee of 1E	1298:1309	Thus, the ee of 1E was low in solution and suspension, but suddenly leaped at the phase border of flowing gel and rigid gel to give the highest ee of 22–24%, which are much higher than those obtained with CD-NSs (6–12% ee), revealing the positive roles of the chiral void space formed upon gelation of the crosslinked saccharide polymer.
25582492	7	56	with	case	1219:1222	arg1	CD-NS					1264:1268	the cyclodextrin-based nanosponge (CD-NS)	1229:1269	the cyclodextrin-based nanosponge (CD-NS) reported previously	1229:1289	Interestingly, the product's ee was controlled more dramatically by the phase evolved, as was the case with the cyclodextrin-based nanosponge (CD-NS) reported previously.
25582492	2	57	theme	CNN-based	376:384	arg1	polymers					386:393	two CNN-based polymers	372:393	two CNN-based polymers of different degrees of crosslinking, both of which swelled upon soaking in water, acting as a ‘nanosponge’ (NS)	372:506	Cyclic nigerosylnigerose (CNN), a saucer-shaped cyclic tetrasaccharide with a shallow concave surface, was reacted with pyromellitic dianhydride in 1:2 and 1:4 ratios to give two CNN-based polymers of different degrees of crosslinking, both of which swelled upon soaking in water, acting as a ‘nanosponge’ (NS).
25582492	2	58	from	dianhydride	330:340	arg1	1:2					345:347	1:2	345:347	1:2	345:347	Cyclic nigerosylnigerose (CNN), a saucer-shaped cyclic tetrasaccharide with a shallow concave surface, was reacted with pyromellitic dianhydride in 1:2 and 1:4 ratios to give two CNN-based polymers of different degrees of crosslinking, both of which swelled upon soaking in water, acting as a ‘nanosponge’ (NS).
25582492	2	58	from	dianhydride	330:340	arg1	ratios					357:362	1:4 ratios	353:362	1:4 ratios	353:362	Cyclic nigerosylnigerose (CNN), a saucer-shaped cyclic tetrasaccharide with a shallow concave surface, was reacted with pyromellitic dianhydride in 1:2 and 1:4 ratios to give two CNN-based polymers of different degrees of crosslinking, both of which swelled upon soaking in water, acting as a ‘nanosponge’ (NS).
25582492	8	59	theme	%	1447:1447	arg1	ee					1436:1437	the highest ee	1424:1437	the highest ee of 22–24%, which are much higher than those obtained with CD-NSs (6–12% ee), revealing the positive roles of the chiral void space formed upon gelation of the crosslinked saccharide polymer	1424:1627	Thus, the ee of 1E was low in solution and suspension, but suddenly leaped at the phase border of flowing gel and rigid gel to give the highest ee of 22–24%, which are much higher than those obtained with CD-NSs (6–12% ee), revealing the positive roles of the chiral void space formed upon gelation of the crosslinked saccharide polymer.
25582492	4	60	theme	-cyclooctene	758:769	arg1	photoisomerization					733:750	the enantiodifferentiating photoisomerization	706:750	the enantiodifferentiating photoisomerization of (Z)-cyclooctene (1Z) to chiral (E)-isomer (1E)	706:800	The CNN-NSs thus prepared effectively mediated the enantiodifferentiating photoisomerization of (Z)-cyclooctene (1Z) to chiral (E)-isomer (1E).
25582492	1	61	theme	cyclic	124:129	arg1	nanosponges					155:165	cyclic nigerosylnigerose-based nanosponges	124:165	cyclic nigerosylnigerose-based nanosponges crosslinked by pyromellitate	124:194	Enantiodifferentiating photoisomerization of (Z)-cyclooctene sensitized by cyclic nigerosylnigerose-based nanosponges crosslinked by pyromellitate.
25582492	2	62	theme	pyromellitic	317:328	arg1	dianhydride					330:340	pyromellitic dianhydride	317:340	pyromellitic dianhydride in 1:2 and 1:4 ratios to give two CNN-based polymers of different degrees of crosslinking, both of which swelled upon soaking in water, acting as a ‘nanosponge’ (NS)	317:506	Cyclic nigerosylnigerose (CNN), a saucer-shaped cyclic tetrasaccharide with a shallow concave surface, was reacted with pyromellitic dianhydride in 1:2 and 1:4 ratios to give two CNN-based polymers of different degrees of crosslinking, both of which swelled upon soaking in water, acting as a ‘nanosponge’ (NS).
25582492	2	63	theme	cyclic	245:250	arg1	nigerosylnigerose					204:220	Cyclic nigerosylnigerose	197:220	Cyclic nigerosylnigerose (CNN)	197:226	Cyclic nigerosylnigerose (CNN), a saucer-shaped cyclic tetrasaccharide with a shallow concave surface, was reacted with pyromellitic dianhydride in 1:2 and 1:4 ratios to give two CNN-based polymers of different degrees of crosslinking, both of which swelled upon soaking in water, acting as a ‘nanosponge’ (NS).
25582492	2	63	theme	cyclic	245:250	arg1	tetrasaccharide					252:266	a saucer-shaped cyclic tetrasaccharide	229:266	a saucer-shaped cyclic tetrasaccharide with a shallow concave surface	229:297	Cyclic nigerosylnigerose (CNN), a saucer-shaped cyclic tetrasaccharide with a shallow concave surface, was reacted with pyromellitic dianhydride in 1:2 and 1:4 ratios to give two CNN-based polymers of different degrees of crosslinking, both of which swelled upon soaking in water, acting as a ‘nanosponge’ (NS).
25582492	1	64	theme	Enantiodifferentiating	49:70	arg1	photoisomerization					72:89	Enantiodifferentiating photoisomerization	49:89	Enantiodifferentiating photoisomerization of (Z)-cyclooctene	49:108	Enantiodifferentiating photoisomerization of (Z)-cyclooctene sensitized by cyclic nigerosylnigerose-based nanosponges crosslinked by pyromellitate.
25582492	8	65	theme	crosslinked	1598:1608	arg1	polymer					1621:1627	the crosslinked saccharide polymer	1594:1627	the crosslinked saccharide polymer	1594:1627	Thus, the ee of 1E was low in solution and suspension, but suddenly leaped at the phase border of flowing gel and rigid gel to give the highest ee of 22–24%, which are much higher than those obtained with CD-NSs (6–12% ee), revealing the positive roles of the chiral void space formed upon gelation of the crosslinked saccharide polymer.
25582492	6	66	theme	chiral	1053:1058	arg1	sense					1060:1064	the chiral sense	1049:1064	the chiral sense of 1E	1049:1070	In isotropic solution, the enantioselectivity was generally low (−4% to +6% ee) but the chiral sense of 1E was inverted by increasing the methanol content.
25582492	6	67	theme	1E	1069:1070	arg1	sense					1060:1064	the chiral sense	1049:1064	the chiral sense of 1E	1049:1070	In isotropic solution, the enantioselectivity was generally low (−4% to +6% ee) but the chiral sense of 1E was inverted by increasing the methanol content.
25582492	2	68	theme	saucer-shaped	231:243	arg1	nigerosylnigerose					204:220	Cyclic nigerosylnigerose	197:220	Cyclic nigerosylnigerose (CNN)	197:226	Cyclic nigerosylnigerose (CNN), a saucer-shaped cyclic tetrasaccharide with a shallow concave surface, was reacted with pyromellitic dianhydride in 1:2 and 1:4 ratios to give two CNN-based polymers of different degrees of crosslinking, both of which swelled upon soaking in water, acting as a ‘nanosponge’ (NS).
25582492	2	68	theme	saucer-shaped	231:243	arg1	tetrasaccharide					252:266	a saucer-shaped cyclic tetrasaccharide	229:266	a saucer-shaped cyclic tetrasaccharide with a shallow concave surface	229:297	Cyclic nigerosylnigerose (CNN), a saucer-shaped cyclic tetrasaccharide with a shallow concave surface, was reacted with pyromellitic dianhydride in 1:2 and 1:4 ratios to give two CNN-based polymers of different degrees of crosslinking, both of which swelled upon soaking in water, acting as a ‘nanosponge’ (NS).
25582492	4	69	dep	prepared	676:683	arg1	mediated					697:704	mediated	697:704	prepared effectively mediated the enantiodifferentiating photoisomerization of (Z)-cyclooctene (1Z) to chiral (E)-isomer (1E)	676:800	The CNN-NSs thus prepared effectively mediated the enantiodifferentiating photoisomerization of (Z)-cyclooctene (1Z) to chiral (E)-isomer (1E).
25582492	6	70	theme	−4	1030:1031	arg1	%					1039:1039	%	1039:1039	%	1039:1039	In isotropic solution, the enantioselectivity was generally low (−4% to +6% ee) but the chiral sense of 1E was inverted by increasing the methanol content.
25582492	3	71	theme	several	527:533	arg1	phases					535:540	several phases	527:540	several phases from isotropic solution to flowing and rigid gels	527:590	These NSs evolved several phases from isotropic solution to flowing and rigid gels via suspension by gradually increasing the concentration in water.
25582492	8	72	theme	6–12	1505:1508	arg1	CD-NSs					1497:1502	CD-NSs	1497:1502	CD-NSs (6–12% ee)	1497:1513	Thus, the ee of 1E was low in solution and suspension, but suddenly leaped at the phase border of flowing gel and rigid gel to give the highest ee of 22–24%, which are much higher than those obtained with CD-NSs (6–12% ee), revealing the positive roles of the chiral void space formed upon gelation of the crosslinked saccharide polymer.
25582492	8	72	theme	6–12	1505:1508	arg1	ee					1511:1512	6–12% ee	1505:1512	6–12% ee	1505:1512	Thus, the ee of 1E was low in solution and suspension, but suddenly leaped at the phase border of flowing gel and rigid gel to give the highest ee of 22–24%, which are much higher than those obtained with CD-NSs (6–12% ee), revealing the positive roles of the chiral void space formed upon gelation of the crosslinked saccharide polymer.
25582492	8	73	theme	polymer	1621:1627	arg1	gelation					1582:1589	gelation	1582:1589	gelation of the crosslinked saccharide polymer	1582:1627	Thus, the ee of 1E was low in solution and suspension, but suddenly leaped at the phase border of flowing gel and rigid gel to give the highest ee of 22–24%, which are much higher than those obtained with CD-NSs (6–12% ee), revealing the positive roles of the chiral void space formed upon gelation of the crosslinked saccharide polymer.
25582492	2	74	theme	degrees	408:414	arg1	polymers					386:393	two CNN-based polymers	372:393	two CNN-based polymers of different degrees of crosslinking, both of which swelled upon soaking in water, acting as a ‘nanosponge’ (NS)	372:506	Cyclic nigerosylnigerose (CNN), a saucer-shaped cyclic tetrasaccharide with a shallow concave surface, was reacted with pyromellitic dianhydride in 1:2 and 1:4 ratios to give two CNN-based polymers of different degrees of crosslinking, both of which swelled upon soaking in water, acting as a ‘nanosponge’ (NS).
24721094	0	0	theme	cell	94:97	arg1	formation					99:107	macrophage-derived foam cell formation	70:107	macrophage-derived foam cell formation	70:107	Jellyfish skin polysaccharides: extraction and inhibitory activity on macrophage-derived foam cell formation.
24721094	5	1	theme	%	618:618	arg1	concentration					586:598	final concentration	580:598	final concentration of 40%, 60% and 80% in turn	580:626	After precipitation with ethanol at final concentration of 40%, 60% and 80% in turn, three polysaccharide fractions of JSP1, JSP2 and JSP3 were obtained from JSP, respectively.
24721094	7	2	theme	oxidized	977:984	arg1	oxLDL					1011:1015	oxLDL	1011:1015	oxLDL	1011:1015	In addition, JSP3 showed strong inhibitory effects on oxidized low-density lipoprotein (oxLDL) induced conversion of macrophages into foam cells, which possibly attributed to the down-regulation of some atherogenesis-related gene expressions.
24721094	7	2	theme	oxidized	977:984	arg1	lipoprotein					998:1008	oxidized low-density lipoprotein	977:1008	oxidized low-density lipoprotein (oxLDL)	977:1016	In addition, JSP3 showed strong inhibitory effects on oxidized low-density lipoprotein (oxLDL) induced conversion of macrophages into foam cells, which possibly attributed to the down-regulation of some atherogenesis-related gene expressions.
24721094	0	3	theme	foam	89:92	arg1	formation					99:107	macrophage-derived foam cell formation	70:107	macrophage-derived foam cell formation	70:107	Jellyfish skin polysaccharides: extraction and inhibitory activity on macrophage-derived foam cell formation.
24721094	6	4	theme	monosaccharide	835:848	arg1	composition					850:860	monosaccharide composition	835:860	monosaccharide composition	835:860	The three fractions exhibited different physicochemical properties with respect to molecular weight distribution, monosaccharide composition, infrared absorption spectra, and glycosyl bond composition.
24721094	5	5	theme	%	605:605	arg1	concentration					586:598	final concentration	580:598	final concentration of 40%, 60% and 80% in turn	580:626	After precipitation with ethanol at final concentration of 40%, 60% and 80% in turn, three polysaccharide fractions of JSP1, JSP2 and JSP3 were obtained from JSP, respectively.
24721094	5	6	theme	polysaccharide	635:648	arg1	fractions					650:658	three polysaccharide fractions	629:658	three polysaccharide fractions of JSP1, JSP2 and JSP3	629:681	After precipitation with ethanol at final concentration of 40%, 60% and 80% in turn, three polysaccharide fractions of JSP1, JSP2 and JSP3 were obtained from JSP, respectively.
24721094	7	7	theme	gene	1148:1151	arg1	expressions					1153:1163	some atherogenesis-related gene expressions	1121:1163	some atherogenesis-related gene expressions	1121:1163	In addition, JSP3 showed strong inhibitory effects on oxidized low-density lipoprotein (oxLDL) induced conversion of macrophages into foam cells, which possibly attributed to the down-regulation of some atherogenesis-related gene expressions.
24721094	0	8	from	activity	58:65	arg1	formation					99:107	macrophage-derived foam cell formation	70:107	macrophage-derived foam cell formation	70:107	Jellyfish skin polysaccharides: extraction and inhibitory activity on macrophage-derived foam cell formation.
24721094	7	9	theme	foam	1057:1060	arg1	cells					1062:1066	foam cells	1057:1066	foam cells	1057:1066	In addition, JSP3 showed strong inhibitory effects on oxidized low-density lipoprotein (oxLDL) induced conversion of macrophages into foam cells, which possibly attributed to the down-regulation of some atherogenesis-related gene expressions.
24721094	1	10	used	used	157:160	arg2	methodology					141:151	response surface methodology	124:151	response surface methodology	124:151	In this work, response surface methodology was used to determine optimum conditions for extraction of polysaccharides from jellyfish skin (JSP).
24721094	2	11	theme	water	311:315	arg1	1:7.5					323:327	water ratio 1:7.5	311:327	water ratio 1:7.5 (w/v)	311:333	The optimum parameters were found to be raw material to water ratio 1:7.5 (w/v), extraction temperature 100°C and extraction time 4h.
24721094	2	11	theme	water	311:315	arg1	w/v					330:332	w/v	330:332	w/v	330:332	The optimum parameters were found to be raw material to water ratio 1:7.5 (w/v), extraction temperature 100°C and extraction time 4h.
24721094	5	12	theme	JSP3	678:681	arg1	fractions					650:658	three polysaccharide fractions	629:658	three polysaccharide fractions of JSP1, JSP2 and JSP3	629:681	After precipitation with ethanol at final concentration of 40%, 60% and 80% in turn, three polysaccharide fractions of JSP1, JSP2 and JSP3 were obtained from JSP, respectively.
24721094	6	13	theme	weight	814:819	arg1	distribution					821:832	molecular weight distribution	804:832	molecular weight distribution	804:832	The three fractions exhibited different physicochemical properties with respect to molecular weight distribution, monosaccharide composition, infrared absorption spectra, and glycosyl bond composition.
24721094	5	14	from	turn	623:626	arg1	concentration					586:598	final concentration	580:598	final concentration of 40%, 60% and 80% in turn	580:626	After precipitation with ethanol at final concentration of 40%, 60% and 80% in turn, three polysaccharide fractions of JSP1, JSP2 and JSP3 were obtained from JSP, respectively.
24721094	7	15	dep	showed	941:946	arg1	induced					1018:1024	induced	1018:1024	showed strong inhibitory effects on oxidized low-density lipoprotein (oxLDL) induced conversion of macrophages into foam cells, which possibly attributed to the down-regulation of some atherogenesis-related gene expressions	941:1163	In addition, JSP3 showed strong inhibitory effects on oxidized low-density lipoprotein (oxLDL) induced conversion of macrophages into foam cells, which possibly attributed to the down-regulation of some atherogenesis-related gene expressions.
24721094	2	16	theme	extraction	369:378	arg1	4h					385:386	extraction time 4h	369:386	extraction time 4h	369:386	The optimum parameters were found to be raw material to water ratio 1:7.5 (w/v), extraction temperature 100°C and extraction time 4h.
24721094	5	17	theme	JSP1	663:666	arg1	fractions					650:658	three polysaccharide fractions	629:658	three polysaccharide fractions of JSP1, JSP2 and JSP3	629:681	After precipitation with ethanol at final concentration of 40%, 60% and 80% in turn, three polysaccharide fractions of JSP1, JSP2 and JSP3 were obtained from JSP, respectively.
24721094	6	18	theme	molecular	804:812	arg1	distribution					821:832	molecular weight distribution	804:832	molecular weight distribution	804:832	The three fractions exhibited different physicochemical properties with respect to molecular weight distribution, monosaccharide composition, infrared absorption spectra, and glycosyl bond composition.
24721094	4	19	from	Papain	447:452	arg1	combination					467:477	combination	467:477	combination with Sevag reagent	467:496	Papain (15 U/mL) in combination with Sevag reagent was beneficial in removing proteins from JSP.
24721094	5	20	from	%	618:618	arg1	turn					623:626	turn	623:626	turn	623:626	After precipitation with ethanol at final concentration of 40%, 60% and 80% in turn, three polysaccharide fractions of JSP1, JSP2 and JSP3 were obtained from JSP, respectively.
24721094	1	21	theme	optimum	175:181	arg1	conditions					183:192	optimum conditions	175:192	optimum conditions for extraction of polysaccharides from jellyfish skin (JSP)	175:252	In this work, response surface methodology was used to determine optimum conditions for extraction of polysaccharides from jellyfish skin (JSP).
24721094	0	22	theme	skin	10:13	arg1	polysaccharides					15:29	Jellyfish skin polysaccharides	0:29	Jellyfish skin polysaccharides: extraction and inhibitory activity on macrophage-derived foam cell formation.	0:108	Jellyfish skin polysaccharides: extraction and inhibitory activity on macrophage-derived foam cell formation.
24721094	0	22	theme	skin	10:13	arg1	extraction					32:41	extraction	32:41	extraction	32:41	Jellyfish skin polysaccharides: extraction and inhibitory activity on macrophage-derived foam cell formation.
24721094	0	22	theme	skin	10:13	arg1	activity					58:65	inhibitory activity	47:65	inhibitory activity	47:65	Jellyfish skin polysaccharides: extraction and inhibitory activity on macrophage-derived foam cell formation.
24721094	0	23	from	extraction	32:41	arg1	formation					99:107	macrophage-derived foam cell formation	70:107	macrophage-derived foam cell formation	70:107	Jellyfish skin polysaccharides: extraction and inhibitory activity on macrophage-derived foam cell formation.
24721094	0	24	theme	Jellyfish	0:8	arg1	polysaccharides					15:29	Jellyfish skin polysaccharides	0:29	Jellyfish skin polysaccharides: extraction and inhibitory activity on macrophage-derived foam cell formation.	0:108	Jellyfish skin polysaccharides: extraction and inhibitory activity on macrophage-derived foam cell formation.
24721094	0	24	theme	Jellyfish	0:8	arg1	extraction					32:41	extraction	32:41	extraction	32:41	Jellyfish skin polysaccharides: extraction and inhibitory activity on macrophage-derived foam cell formation.
24721094	0	24	theme	Jellyfish	0:8	arg1	activity					58:65	inhibitory activity	47:65	inhibitory activity	47:65	Jellyfish skin polysaccharides: extraction and inhibitory activity on macrophage-derived foam cell formation.
24721094	5	25	from	concentration	586:598	arg1	turn					623:626	turn	623:626	turn	623:626	After precipitation with ethanol at final concentration of 40%, 60% and 80% in turn, three polysaccharide fractions of JSP1, JSP2 and JSP3 were obtained from JSP, respectively.
24721094	5	25	from	concentration	586:598	arg1	ethanol					569:575	ethanol	569:575	ethanol at final concentration of 40%, 60% and 80% in turn	569:626	After precipitation with ethanol at final concentration of 40%, 60% and 80% in turn, three polysaccharide fractions of JSP1, JSP2 and JSP3 were obtained from JSP, respectively.
24721094	6	26	theme	infrared	863:870	arg1	spectra					883:889	infrared absorption spectra	863:889	infrared absorption spectra	863:889	The three fractions exhibited different physicochemical properties with respect to molecular weight distribution, monosaccharide composition, infrared absorption spectra, and glycosyl bond composition.
24721094	2	27	theme	temperature	347:357	arg1	100°C					359:363	extraction temperature 100°C	336:363	extraction temperature 100°C	336:363	The optimum parameters were found to be raw material to water ratio 1:7.5 (w/v), extraction temperature 100°C and extraction time 4h.
24721094	6	28	theme	bond	905:908	arg1	composition					910:920	glycosyl bond composition	896:920	glycosyl bond composition	896:920	The three fractions exhibited different physicochemical properties with respect to molecular weight distribution, monosaccharide composition, infrared absorption spectra, and glycosyl bond composition.
24721094	2	29	theme	raw	295:297	arg1	parameters					267:276	The optimum parameters	255:276	The optimum parameters	255:276	The optimum parameters were found to be raw material to water ratio 1:7.5 (w/v), extraction temperature 100°C and extraction time 4h.
24721094	2	29	theme	raw	295:297	arg1	material					299:306	raw material	295:306	raw material	295:306	The optimum parameters were found to be raw material to water ratio 1:7.5 (w/v), extraction temperature 100°C and extraction time 4h.
24721094	7	30	theme	macrophages	1040:1050	arg1	conversion					1026:1035	conversion	1026:1035	conversion of macrophages into foam cells, which possibly attributed to the down-regulation of some atherogenesis-related gene expressions	1026:1163	In addition, JSP3 showed strong inhibitory effects on oxidized low-density lipoprotein (oxLDL) induced conversion of macrophages into foam cells, which possibly attributed to the down-regulation of some atherogenesis-related gene expressions.
24721094	6	31	theme	glycosyl	896:903	arg1	composition					910:920	glycosyl bond composition	896:920	glycosyl bond composition	896:920	The three fractions exhibited different physicochemical properties with respect to molecular weight distribution, monosaccharide composition, infrared absorption spectra, and glycosyl bond composition.
24721094	1	32	from	extraction	198:207	arg1	JSP					249:251	JSP	249:251	JSP	249:251	In this work, response surface methodology was used to determine optimum conditions for extraction of polysaccharides from jellyfish skin (JSP).
24721094	1	32	from	extraction	198:207	arg1	skin					243:246	jellyfish skin	233:246	jellyfish skin (JSP)	233:252	In this work, response surface methodology was used to determine optimum conditions for extraction of polysaccharides from jellyfish skin (JSP).
24721094	4	33	with	combination	467:477	arg1	reagent					490:496	Sevag reagent	484:496	Sevag reagent	484:496	Papain (15 U/mL) in combination with Sevag reagent was beneficial in removing proteins from JSP.
24721094	3	34	theme	JSP	417:419	arg1	yield					421:425	the JSP yield	413:425	the JSP yield	413:425	Under these conditions, the JSP yield reached 1.007 mg/g.
24721094	1	35	theme	polysaccharides	212:226	arg1	extraction					198:207	extraction	198:207	extraction of polysaccharides from jellyfish skin (JSP)	198:252	In this work, response surface methodology was used to determine optimum conditions for extraction of polysaccharides from jellyfish skin (JSP).
24721094	5	36	from	%	605:605	arg1	turn					623:626	turn	623:626	turn	623:626	After precipitation with ethanol at final concentration of 40%, 60% and 80% in turn, three polysaccharide fractions of JSP1, JSP2 and JSP3 were obtained from JSP, respectively.
24721094	7	37	theme	strong	948:953	arg1	effects					966:972	strong inhibitory effects	948:972	strong inhibitory effects on oxidized low-density lipoprotein (oxLDL)	948:1016	In addition, JSP3 showed strong inhibitory effects on oxidized low-density lipoprotein (oxLDL) induced conversion of macrophages into foam cells, which possibly attributed to the down-regulation of some atherogenesis-related gene expressions.
24721094	2	38	theme	time	380:383	arg1	4h					385:386	extraction time 4h	369:386	extraction time 4h	369:386	The optimum parameters were found to be raw material to water ratio 1:7.5 (w/v), extraction temperature 100°C and extraction time 4h.
24721094	7	39	theme	expressions	1153:1163	arg1	down-regulation					1102:1116	the down-regulation	1098:1116	the down-regulation of some atherogenesis-related gene expressions	1098:1163	In addition, JSP3 showed strong inhibitory effects on oxidized low-density lipoprotein (oxLDL) induced conversion of macrophages into foam cells, which possibly attributed to the down-regulation of some atherogenesis-related gene expressions.
24721094	5	40	theme	%	610:610	arg1	concentration					586:598	final concentration	580:598	final concentration of 40%, 60% and 80% in turn	580:626	After precipitation with ethanol at final concentration of 40%, 60% and 80% in turn, three polysaccharide fractions of JSP1, JSP2 and JSP3 were obtained from JSP, respectively.
24721094	6	41	theme	physicochemical	761:775	arg1	properties					777:786	different physicochemical properties	751:786	different physicochemical properties	751:786	The three fractions exhibited different physicochemical properties with respect to molecular weight distribution, monosaccharide composition, infrared absorption spectra, and glycosyl bond composition.
24721094	7	42	theme	inhibitory	955:964	arg1	effects					966:972	strong inhibitory effects	948:972	strong inhibitory effects on oxidized low-density lipoprotein (oxLDL)	948:1016	In addition, JSP3 showed strong inhibitory effects on oxidized low-density lipoprotein (oxLDL) induced conversion of macrophages into foam cells, which possibly attributed to the down-regulation of some atherogenesis-related gene expressions.
24721094	0	43	theme	inhibitory	47:56	arg1	polysaccharides					15:29	Jellyfish skin polysaccharides	0:29	Jellyfish skin polysaccharides: extraction and inhibitory activity on macrophage-derived foam cell formation.	0:108	Jellyfish skin polysaccharides: extraction and inhibitory activity on macrophage-derived foam cell formation.
24721094	0	43	theme	inhibitory	47:56	arg1	activity					58:65	inhibitory activity	47:65	inhibitory activity	47:65	Jellyfish skin polysaccharides: extraction and inhibitory activity on macrophage-derived foam cell formation.
24721094	6	44	theme	absorption	872:881	arg1	spectra					883:889	infrared absorption spectra	863:889	infrared absorption spectra	863:889	The three fractions exhibited different physicochemical properties with respect to molecular weight distribution, monosaccharide composition, infrared absorption spectra, and glycosyl bond composition.
24721094	7	45	theme	low-density	986:996	arg1	oxLDL					1011:1015	oxLDL	1011:1015	oxLDL	1011:1015	In addition, JSP3 showed strong inhibitory effects on oxidized low-density lipoprotein (oxLDL) induced conversion of macrophages into foam cells, which possibly attributed to the down-regulation of some atherogenesis-related gene expressions.
24721094	7	45	theme	low-density	986:996	arg1	lipoprotein					998:1008	oxidized low-density lipoprotein	977:1008	oxidized low-density lipoprotein (oxLDL)	977:1016	In addition, JSP3 showed strong inhibitory effects on oxidized low-density lipoprotein (oxLDL) induced conversion of macrophages into foam cells, which possibly attributed to the down-regulation of some atherogenesis-related gene expressions.
24721094	6	46	theme	different	751:759	arg1	properties					777:786	different physicochemical properties	751:786	different physicochemical properties	751:786	The three fractions exhibited different physicochemical properties with respect to molecular weight distribution, monosaccharide composition, infrared absorption spectra, and glycosyl bond composition.
24721094	5	47	theme	final	580:584	arg1	concentration					586:598	final concentration	580:598	final concentration of 40%, 60% and 80% in turn	580:626	After precipitation with ethanol at final concentration of 40%, 60% and 80% in turn, three polysaccharide fractions of JSP1, JSP2 and JSP3 were obtained from JSP, respectively.
24721094	0	48	link	macrophage-derived	70:87	arg1	formation					99:107	macrophage-derived foam cell formation	70:107	macrophage-derived foam cell formation	70:107	Jellyfish skin polysaccharides: extraction and inhibitory activity on macrophage-derived foam cell formation.
24721094	4	49	theme	Sevag	484:488	arg1	reagent					490:496	Sevag reagent	484:496	Sevag reagent	484:496	Papain (15 U/mL) in combination with Sevag reagent was beneficial in removing proteins from JSP.
24721094	2	50	theme	optimum	259:265	arg1	parameters					267:276	The optimum parameters	255:276	The optimum parameters	255:276	The optimum parameters were found to be raw material to water ratio 1:7.5 (w/v), extraction temperature 100°C and extraction time 4h.
24721094	2	50	theme	optimum	259:265	arg1	material					299:306	raw material	295:306	raw material	295:306	The optimum parameters were found to be raw material to water ratio 1:7.5 (w/v), extraction temperature 100°C and extraction time 4h.
24721094	1	51	theme	response	124:131	arg1	methodology					141:151	response surface methodology	124:151	response surface methodology	124:151	In this work, response surface methodology was used to determine optimum conditions for extraction of polysaccharides from jellyfish skin (JSP).
24721094	1	52	theme	jellyfish	233:241	arg1	JSP					249:251	JSP	249:251	JSP	249:251	In this work, response surface methodology was used to determine optimum conditions for extraction of polysaccharides from jellyfish skin (JSP).
24721094	1	52	theme	jellyfish	233:241	arg1	skin					243:246	jellyfish skin	233:246	jellyfish skin (JSP)	233:252	In this work, response surface methodology was used to determine optimum conditions for extraction of polysaccharides from jellyfish skin (JSP).
24721094	1	53	from	skin	243:246	arg1	polysaccharides					212:226	polysaccharides	212:226	polysaccharides from jellyfish skin (JSP)	212:252	In this work, response surface methodology was used to determine optimum conditions for extraction of polysaccharides from jellyfish skin (JSP).
24721094	1	53	from	skin	243:246	arg1	extraction					198:207	extraction	198:207	extraction of polysaccharides from jellyfish skin (JSP)	198:252	In this work, response surface methodology was used to determine optimum conditions for extraction of polysaccharides from jellyfish skin (JSP).
24721094	0	54	dep	polysaccharides	15:29	arg1	polysaccharides					15:29	Jellyfish skin polysaccharides	0:29	Jellyfish skin polysaccharides: extraction and inhibitory activity on macrophage-derived foam cell formation.	0:108	Jellyfish skin polysaccharides: extraction and inhibitory activity on macrophage-derived foam cell formation.
24721094	0	54	dep	polysaccharides	15:29	arg1	extraction					32:41	extraction	32:41	extraction	32:41	Jellyfish skin polysaccharides: extraction and inhibitory activity on macrophage-derived foam cell formation.
24721094	0	54	dep	polysaccharides	15:29	arg1	activity					58:65	inhibitory activity	47:65	inhibitory activity	47:65	Jellyfish skin polysaccharides: extraction and inhibitory activity on macrophage-derived foam cell formation.
24721094	1	55	theme	surface	133:139	arg1	methodology					141:151	response surface methodology	124:151	response surface methodology	124:151	In this work, response surface methodology was used to determine optimum conditions for extraction of polysaccharides from jellyfish skin (JSP).
24721094	2	56	theme	ratio	317:321	arg1	1:7.5					323:327	water ratio 1:7.5	311:327	water ratio 1:7.5 (w/v)	311:333	The optimum parameters were found to be raw material to water ratio 1:7.5 (w/v), extraction temperature 100°C and extraction time 4h.
24721094	2	56	theme	ratio	317:321	arg1	w/v					330:332	w/v	330:332	w/v	330:332	The optimum parameters were found to be raw material to water ratio 1:7.5 (w/v), extraction temperature 100°C and extraction time 4h.
24721094	0	57	theme	macrophage-derived	70:87	arg1	formation					99:107	macrophage-derived foam cell formation	70:107	macrophage-derived foam cell formation	70:107	Jellyfish skin polysaccharides: extraction and inhibitory activity on macrophage-derived foam cell formation.
24721094	5	58	with	precipitation	550:562	arg1	ethanol					569:575	ethanol	569:575	ethanol at final concentration of 40%, 60% and 80% in turn	569:626	After precipitation with ethanol at final concentration of 40%, 60% and 80% in turn, three polysaccharide fractions of JSP1, JSP2 and JSP3 were obtained from JSP, respectively.
24721094	2	59	theme	extraction	336:345	arg1	100°C					359:363	extraction temperature 100°C	336:363	extraction temperature 100°C	336:363	The optimum parameters were found to be raw material to water ratio 1:7.5 (w/v), extraction temperature 100°C and extraction time 4h.
24721094	5	60	from	%	610:610	arg1	turn					623:626	turn	623:626	turn	623:626	After precipitation with ethanol at final concentration of 40%, 60% and 80% in turn, three polysaccharide fractions of JSP1, JSP2 and JSP3 were obtained from JSP, respectively.
24721094	5	61	theme	JSP2	669:672	arg1	fractions					650:658	three polysaccharide fractions	629:658	three polysaccharide fractions of JSP1, JSP2 and JSP3	629:681	After precipitation with ethanol at final concentration of 40%, 60% and 80% in turn, three polysaccharide fractions of JSP1, JSP2 and JSP3 were obtained from JSP, respectively.
24721094	7	62	theme	atherogenesis-related	1126:1146	arg1	expressions					1153:1163	some atherogenesis-related gene expressions	1121:1163	some atherogenesis-related gene expressions	1121:1163	In addition, JSP3 showed strong inhibitory effects on oxidized low-density lipoprotein (oxLDL) induced conversion of macrophages into foam cells, which possibly attributed to the down-regulation of some atherogenesis-related gene expressions.
24721094	7	63	from	effects	966:972	arg1	oxLDL					1011:1015	oxLDL	1011:1015	oxLDL	1011:1015	In addition, JSP3 showed strong inhibitory effects on oxidized low-density lipoprotein (oxLDL) induced conversion of macrophages into foam cells, which possibly attributed to the down-regulation of some atherogenesis-related gene expressions.
24721094	7	63	from	effects	966:972	arg1	lipoprotein					998:1008	oxidized low-density lipoprotein	977:1008	oxidized low-density lipoprotein (oxLDL)	977:1016	In addition, JSP3 showed strong inhibitory effects on oxidized low-density lipoprotein (oxLDL) induced conversion of macrophages into foam cells, which possibly attributed to the down-regulation of some atherogenesis-related gene expressions.
25727745	1	0	theme	easy	370:373	arg1	production					375:384	the easy production	366:384	the easy production of white hide powder/cellulose composite	366:425	Gemini imidazolium ionic liquid, 3,3'-[1,2-ethanediylbis (oxy-2,1-ethanediyl)]-bis[1-methyl-imidazolium]-dibromide ([C6O2(mim)2][Br]2), was used for the dissolution and regeneration of white hide powder (from pigskin), and blend white hide powder with cellulose for the easy production of white hide powder/cellulose composite.
25727745	6	1	theme	ionic	897:901	arg1	liquids					903:909	common ionic liquids	890:909	common ionic liquids	890:909	The dissolution time of [C6O2(mim)2][Br]2 for white hide powder was shorter than those of common ionic liquids.
25727745	1	2	from	regeneration	269:280	arg1	pigskin					309:315	pigskin	309:315	pigskin	309:315	Gemini imidazolium ionic liquid, 3,3'-[1,2-ethanediylbis (oxy-2,1-ethanediyl)]-bis[1-methyl-imidazolium]-dibromide ([C6O2(mim)2][Br]2), was used for the dissolution and regeneration of white hide powder (from pigskin), and blend white hide powder with cellulose for the easy production of white hide powder/cellulose composite.
25727745	3	3	dep	[Br	552:554	arg1	powder					581:586	regenerated white hide powder	558:586	The native white hide powder and [C6O2(mim)2][Br]2 regenerated white hide powder	507:586	The native white hide powder and [C6O2(mim)2][Br]2 regenerated white hide powder were characterized by FT-IR, XRD, DSC-TG and FE-SEM.
25727745	3	3	dep	[Br	552:554	arg1	hide					524:527	hide	524:527	hide powder and [C6O2(mim)	524:549	The native white hide powder and [C6O2(mim)2][Br]2 regenerated white hide powder were characterized by FT-IR, XRD, DSC-TG and FE-SEM.
25727745	3	3	dep	[Br	552:554	arg1	2					550:550	2	550:550	2	550:550	The native white hide powder and [C6O2(mim)2][Br]2 regenerated white hide powder were characterized by FT-IR, XRD, DSC-TG and FE-SEM.
25727745	1	4	with	regeneration	269:280	arg1	cellulose					352:360	cellulose	352:360	cellulose	352:360	Gemini imidazolium ionic liquid, 3,3'-[1,2-ethanediylbis (oxy-2,1-ethanediyl)]-bis[1-methyl-imidazolium]-dibromide ([C6O2(mim)2][Br]2), was used for the dissolution and regeneration of white hide powder (from pigskin), and blend white hide powder with cellulose for the easy production of white hide powder/cellulose composite.
25727745	1	5	from	dissolution	253:263	arg1	pigskin					309:315	pigskin	309:315	pigskin	309:315	Gemini imidazolium ionic liquid, 3,3'-[1,2-ethanediylbis (oxy-2,1-ethanediyl)]-bis[1-methyl-imidazolium]-dibromide ([C6O2(mim)2][Br]2), was used for the dissolution and regeneration of white hide powder (from pigskin), and blend white hide powder with cellulose for the easy production of white hide powder/cellulose composite.
25727745	1	6	theme	hide	395:398	arg1	composite					417:425	white hide powder/cellulose composite	389:425	white hide powder/cellulose composite	389:425	Gemini imidazolium ionic liquid, 3,3'-[1,2-ethanediylbis (oxy-2,1-ethanediyl)]-bis[1-methyl-imidazolium]-dibromide ([C6O2(mim)2][Br]2), was used for the dissolution and regeneration of white hide powder (from pigskin), and blend white hide powder with cellulose for the easy production of white hide powder/cellulose composite.
25727745	1	7	used	used	240:243	arg2	Gemini					100:105	Gemini	100:105	Gemini imidazolium ionic liquid	100:130	Gemini imidazolium ionic liquid, 3,3'-[1,2-ethanediylbis (oxy-2,1-ethanediyl)]-bis[1-methyl-imidazolium]-dibromide ([C6O2(mim)2][Br]2), was used for the dissolution and regeneration of white hide powder (from pigskin), and blend white hide powder with cellulose for the easy production of white hide powder/cellulose composite.
25727745	1	7	used	used	240:243	arg2	-dibromide					204:213	3,3'-[1,2-ethanediylbis (oxy-2,1-ethanediyl)]-bis[1-methyl-imidazolium]-dibromide	133:213	3,3'-[1,2-ethanediylbis (oxy-2,1-ethanediyl)]-bis[1-methyl-imidazolium]-dibromide ([C6O2(mim)2][Br]2)	133:233	Gemini imidazolium ionic liquid, 3,3'-[1,2-ethanediylbis (oxy-2,1-ethanediyl)]-bis[1-methyl-imidazolium]-dibromide ([C6O2(mim)2][Br]2), was used for the dissolution and regeneration of white hide powder (from pigskin), and blend white hide powder with cellulose for the easy production of white hide powder/cellulose composite.
25727745	1	8	with	dissolution	253:263	arg1	cellulose					352:360	cellulose	352:360	cellulose	352:360	Gemini imidazolium ionic liquid, 3,3'-[1,2-ethanediylbis (oxy-2,1-ethanediyl)]-bis[1-methyl-imidazolium]-dibromide ([C6O2(mim)2][Br]2), was used for the dissolution and regeneration of white hide powder (from pigskin), and blend white hide powder with cellulose for the easy production of white hide powder/cellulose composite.
25727745	1	9	theme	composite	417:425	arg1	production					375:384	the easy production	366:384	the easy production of white hide powder/cellulose composite	366:425	Gemini imidazolium ionic liquid, 3,3'-[1,2-ethanediylbis (oxy-2,1-ethanediyl)]-bis[1-methyl-imidazolium]-dibromide ([C6O2(mim)2][Br]2), was used for the dissolution and regeneration of white hide powder (from pigskin), and blend white hide powder with cellulose for the easy production of white hide powder/cellulose composite.
25727745	5	10	from	120°C	793:797	arg1	powder					776:781	the white hide powder	761:781	the white hide powder	761:781	The dissolution time was 55 min when the white hide powder was 8% at 120°C.
25727745	5	10	from	120°C	793:797	arg1	%					788:788	8%	787:788	8%	787:788	The dissolution time was 55 min when the white hide powder was 8% at 120°C.
25727745	9	11	dep	[Br	1253:1255	arg1	2					1251:1251	2	1251:1251	2	1251:1251	White hide powder/cellulose composites were successfully dissolved in [C6O2(mim)2][Br]2.
25727745	1	12	theme	blend	323:327	arg1	powder					340:345	blend white hide powder	323:345	blend white hide powder	323:345	Gemini imidazolium ionic liquid, 3,3'-[1,2-ethanediylbis (oxy-2,1-ethanediyl)]-bis[1-methyl-imidazolium]-dibromide ([C6O2(mim)2][Br]2), was used for the dissolution and regeneration of white hide powder (from pigskin), and blend white hide powder with cellulose for the easy production of white hide powder/cellulose composite.
25727745	2	13	theme	mim	482:484	arg1	[Br					488:490	[C6O2(mim)2][Br	476:490	[C6O2(mim)2][Br	476:490	Dissolution performance of white hide powder in [C6O2(mim)2][Br]2 was studied.
25727745	8	14	theme	[Br	1092:1094	arg1	mechanism					1034:1042	The possible mechanism	1021:1042	The possible mechanism of white hide powder dissolution in [C6O2(mim)2][Br]2 and the regeneration of white hide powder in methanol	1021:1150	The possible mechanism of white hide powder dissolution in [C6O2(mim)2][Br]2 and the regeneration of white hide powder in methanol had been proposed.
25727745	11	15	dep	strength	1354:1361	arg1	The					1342:1344	The	1342:1344	The	1342:1344	The tensile strength, and elongation at break of white hide powder/cellulose composite films were tested.
25727745	8	16	from	powder	1133:1138	arg1	methanol					1143:1150	methanol	1143:1150	methanol	1143:1150	The possible mechanism of white hide powder dissolution in [C6O2(mim)2][Br]2 and the regeneration of white hide powder in methanol had been proposed.
25727745	0	17	from	Gemini	67:72	arg1	composite					54:62	composite	54:62	composite	54:62	Dissolution and regeneration of hide powder/cellulose composite in Gemini imidazolium ionic liquid.
25727745	11	18	theme	composite	1419:1427	arg1	films					1429:1433	white hide powder/cellulose composite films	1391:1433	white hide powder/cellulose composite films	1391:1433	The tensile strength, and elongation at break of white hide powder/cellulose composite films were tested.
25727745	4	19	theme	white	705:709	arg1	powder					716:721	white hide powder	705:721	white hide powder	705:721	The results showed that [C6O2(mim)2][Br]2 was a good solvent to white hide powder.
25727745	1	20	theme	[1,2-ethanediylbis	138:155	arg1	-dibromide					204:213	3,3'-[1,2-ethanediylbis (oxy-2,1-ethanediyl)]-bis[1-methyl-imidazolium]-dibromide	133:213	3,3'-[1,2-ethanediylbis (oxy-2,1-ethanediyl)]-bis[1-methyl-imidazolium]-dibromide ([C6O2(mim)2][Br]2)	133:233	Gemini imidazolium ionic liquid, 3,3'-[1,2-ethanediylbis (oxy-2,1-ethanediyl)]-bis[1-methyl-imidazolium]-dibromide ([C6O2(mim)2][Br]2), was used for the dissolution and regeneration of white hide powder (from pigskin), and blend white hide powder with cellulose for the easy production of white hide powder/cellulose composite.
25727745	1	20	theme	[1,2-ethanediylbis	138:155	arg1	[Br					228:230	[C6O2(mim)2][Br]2	216:232	[C6O2(mim)2][Br]2	216:232	Gemini imidazolium ionic liquid, 3,3'-[1,2-ethanediylbis (oxy-2,1-ethanediyl)]-bis[1-methyl-imidazolium]-dibromide ([C6O2(mim)2][Br]2), was used for the dissolution and regeneration of white hide powder (from pigskin), and blend white hide powder with cellulose for the easy production of white hide powder/cellulose composite.
25727745	1	20	theme	[1,2-ethanediylbis	138:155	arg1	Gemini					100:105	Gemini	100:105	Gemini imidazolium ionic liquid	100:130	Gemini imidazolium ionic liquid, 3,3'-[1,2-ethanediylbis (oxy-2,1-ethanediyl)]-bis[1-methyl-imidazolium]-dibromide ([C6O2(mim)2][Br]2), was used for the dissolution and regeneration of white hide powder (from pigskin), and blend white hide powder with cellulose for the easy production of white hide powder/cellulose composite.
25727745	2	21	theme	white	455:459	arg1	powder					466:471	white hide powder	455:471	white hide powder	455:471	Dissolution performance of white hide powder in [C6O2(mim)2][Br]2 was studied.
25727745	7	22	theme	white	944:948	arg1	powder					955:960	white hide powder	944:960	white hide powder	944:960	The triple helical structure of white hide powder was partly destroyed during [C6O2(mim)2][Br]2 dissolution.
25727745	3	23	theme	white	518:522	arg1	[Br					552:554	The native white hide powder and [C6O2(mim)2][Br]2	507:556	The native white hide powder and [C6O2(mim)2][Br]2 regenerated white hide powder	507:586	The native white hide powder and [C6O2(mim)2][Br]2 regenerated white hide powder were characterized by FT-IR, XRD, DSC-TG and FE-SEM.
25727745	1	24	dep	dissolution	253:263	arg1	the					249:251	the	249:251	the	249:251	Gemini imidazolium ionic liquid, 3,3'-[1,2-ethanediylbis (oxy-2,1-ethanediyl)]-bis[1-methyl-imidazolium]-dibromide ([C6O2(mim)2][Br]2), was used for the dissolution and regeneration of white hide powder (from pigskin), and blend white hide powder with cellulose for the easy production of white hide powder/cellulose composite.
25727745	8	25	from	[Br	1092:1094	arg1	methanol					1143:1150	methanol	1143:1150	methanol	1143:1150	The possible mechanism of white hide powder dissolution in [C6O2(mim)2][Br]2 and the regeneration of white hide powder in methanol had been proposed.
25727745	2	26	theme	[C6O2	476:480	arg1	[Br					488:490	[C6O2(mim)2][Br	476:490	[C6O2(mim)2][Br	476:490	Dissolution performance of white hide powder in [C6O2(mim)2][Br]2 was studied.
25727745	7	27	theme	triple	916:921	arg1	structure					931:939	The triple helical structure	912:939	The triple helical structure of white hide powder	912:960	The triple helical structure of white hide powder was partly destroyed during [C6O2(mim)2][Br]2 dissolution.
25727745	12	28	theme	collagen-based	1546:1559	arg1	engineering					1568:1578	collagen-based tissue engineering	1546:1578	collagen-based tissue engineering	1546:1578	This work demonstrated that the white hide powder/cellulose composite exhibited some potential in collagen-based tissue engineering.
25727745	2	29	theme	powder	466:471	arg1	performance					440:450	Dissolution performance	428:450	Dissolution performance of white hide powder in [C6O2(mim)2][Br]2	428:492	Dissolution performance of white hide powder in [C6O2(mim)2][Br]2 was studied.
25727745	0	30	from	composite	54:62	arg1	Gemini					67:72	Gemini	67:72	Gemini	67:72	Dissolution and regeneration of hide powder/cellulose composite in Gemini imidazolium ionic liquid.
25727745	1	31	theme	white	285:289	arg1	powder					296:301	powder	296:301	powder (from pigskin)	296:316	Gemini imidazolium ionic liquid, 3,3'-[1,2-ethanediylbis (oxy-2,1-ethanediyl)]-bis[1-methyl-imidazolium]-dibromide ([C6O2(mim)2][Br]2), was used for the dissolution and regeneration of white hide powder (from pigskin), and blend white hide powder with cellulose for the easy production of white hide powder/cellulose composite.
25727745	12	32	theme	powder/cellulose	1491:1506	arg1	composite					1508:1516	the white hide powder/cellulose composite	1476:1516	the white hide powder/cellulose composite	1476:1516	This work demonstrated that the white hide powder/cellulose composite exhibited some potential in collagen-based tissue engineering.
25727745	5	33	theme	white	765:769	arg1	powder					776:781	the white hide powder	761:781	the white hide powder	761:781	The dissolution time was 55 min when the white hide powder was 8% at 120°C.
25727745	5	33	theme	white	765:769	arg1	%					788:788	8%	787:788	8%	787:788	The dissolution time was 55 min when the white hide powder was 8% at 120°C.
25727745	6	34	theme	mim	830:832	arg1	[Br					836:838	[C6O2(mim)2][Br]2	824:840	[C6O2(mim)2][Br]2 for white hide powder	824:862	The dissolution time of [C6O2(mim)2][Br]2 for white hide powder was shorter than those of common ionic liquids.
25727745	8	35	theme	white	1047:1051	arg1	[Br					1092:1094	white hide powder dissolution in [C6O2(mim)2][Br]2	1047:1096	white hide powder dissolution in [C6O2(mim)2][Br]2	1047:1096	The possible mechanism of white hide powder dissolution in [C6O2(mim)2][Br]2 and the regeneration of white hide powder in methanol had been proposed.
25727745	7	36	dep	powder	955:960	arg1	hide					950:953	hide	950:953	hide	950:953	The triple helical structure of white hide powder was partly destroyed during [C6O2(mim)2][Br]2 dissolution.
25727745	6	37	theme	[C6O2	824:828	arg1	[Br					836:838	[C6O2(mim)2][Br]2	824:840	[C6O2(mim)2][Br]2 for white hide powder	824:862	The dissolution time of [C6O2(mim)2][Br]2 for white hide powder was shorter than those of common ionic liquids.
25727745	8	38	theme	powder	1058:1063	arg1	dissolution					1065:1075	powder dissolution	1058:1075	powder dissolution in [C6O2(mim)	1058:1089	The possible mechanism of white hide powder dissolution in [C6O2(mim)2][Br]2 and the regeneration of white hide powder in methanol had been proposed.
25727745	10	39	theme	powder/cellulose	1290:1305	arg1	film					1307:1310	white hide powder/cellulose film	1279:1310	white hide powder/cellulose film	1279:1310	The performance of white hide powder/cellulose film was measured by FT-IR and TG.
25727745	1	40	dep	powder	296:301	arg1	hide					291:294	hide	291:294	hide	291:294	Gemini imidazolium ionic liquid, 3,3'-[1,2-ethanediylbis (oxy-2,1-ethanediyl)]-bis[1-methyl-imidazolium]-dibromide ([C6O2(mim)2][Br]2), was used for the dissolution and regeneration of white hide powder (from pigskin), and blend white hide powder with cellulose for the easy production of white hide powder/cellulose composite.
25727745	6	41	theme	dissolution	804:814	arg1	time					816:819	The dissolution time	800:819	The dissolution time of [C6O2(mim)2][Br]2 for white hide powder	800:862	The dissolution time of [C6O2(mim)2][Br]2 for white hide powder was shorter than those of common ionic liquids.
25727745	6	41	theme	dissolution	804:814	arg1	shorter					868:874	shorter	868:874	shorter	868:874	The dissolution time of [C6O2(mim)2][Br]2 for white hide powder was shorter than those of common ionic liquids.
25727745	4	42	theme	[C6O2	665:669	arg1	[Br					677:679	[C6O2(mim)2][Br]2	665:681	[C6O2(mim)2][Br]2	665:681	The results showed that [C6O2(mim)2][Br]2 was a good solvent to white hide powder.
25727745	4	42	theme	[C6O2	665:669	arg1	solvent					694:700	a good solvent	687:700	a good solvent to white hide powder	687:721	The results showed that [C6O2(mim)2][Br]2 was a good solvent to white hide powder.
25727745	10	43	theme	white	1279:1283	arg1	film					1307:1310	white hide powder/cellulose film	1279:1310	white hide powder/cellulose film	1279:1310	The performance of white hide powder/cellulose film was measured by FT-IR and TG.
25727745	12	44	theme	white	1480:1484	arg1	composite					1508:1516	the white hide powder/cellulose composite	1476:1516	the white hide powder/cellulose composite	1476:1516	This work demonstrated that the white hide powder/cellulose composite exhibited some potential in collagen-based tissue engineering.
25727745	4	45	theme	mim	671:673	arg1	[Br					677:679	[C6O2(mim)2][Br]2	665:681	[C6O2(mim)2][Br]2	665:681	The results showed that [C6O2(mim)2][Br]2 was a good solvent to white hide powder.
25727745	4	45	theme	mim	671:673	arg1	solvent					694:700	a good solvent	687:700	a good solvent to white hide powder	687:721	The results showed that [C6O2(mim)2][Br]2 was a good solvent to white hide powder.
25727745	7	46	dep	dissolution	1008:1018	arg1	2					1000:1000	2	1000:1000	2	1000:1000	The triple helical structure of white hide powder was partly destroyed during [C6O2(mim)2][Br]2 dissolution.
25727745	11	47	dep	white	1391:1395	arg1	hide					1397:1400	hide	1397:1400	hide	1397:1400	The tensile strength, and elongation at break of white hide powder/cellulose composite films were tested.
25727745	6	48	theme	white	846:850	arg1	powder					857:862	white hide powder	846:862	white hide powder	846:862	The dissolution time of [C6O2(mim)2][Br]2 for white hide powder was shorter than those of common ionic liquids.
25727745	1	49	dep	powder	340:345	arg1	hide					335:338	hide	335:338	hide	335:338	Gemini imidazolium ionic liquid, 3,3'-[1,2-ethanediylbis (oxy-2,1-ethanediyl)]-bis[1-methyl-imidazolium]-dibromide ([C6O2(mim)2][Br]2), was used for the dissolution and regeneration of white hide powder (from pigskin), and blend white hide powder with cellulose for the easy production of white hide powder/cellulose composite.
25727745	6	50	theme	[Br	836:838	arg1	time					816:819	The dissolution time	800:819	The dissolution time of [C6O2(mim)2][Br]2 for white hide powder	800:862	The dissolution time of [C6O2(mim)2][Br]2 for white hide powder was shorter than those of common ionic liquids.
25727745	6	50	theme	[Br	836:838	arg1	shorter					868:874	shorter	868:874	shorter	868:874	The dissolution time of [C6O2(mim)2][Br]2 for white hide powder was shorter than those of common ionic liquids.
25727745	3	51	theme	white	570:574	arg1	powder					581:586	regenerated white hide powder	558:586	The native white hide powder and [C6O2(mim)2][Br]2 regenerated white hide powder	507:586	The native white hide powder and [C6O2(mim)2][Br]2 regenerated white hide powder were characterized by FT-IR, XRD, DSC-TG and FE-SEM.
25727745	8	52	theme	hide	1128:1131	arg1	powder					1133:1138	white hide powder	1122:1138	white hide powder in methanol	1122:1150	The possible mechanism of white hide powder dissolution in [C6O2(mim)2][Br]2 and the regeneration of white hide powder in methanol had been proposed.
25727745	7	53	theme	powder	955:960	arg1	structure					931:939	The triple helical structure	912:939	The triple helical structure of white hide powder	912:960	The triple helical structure of white hide powder was partly destroyed during [C6O2(mim)2][Br]2 dissolution.
25727745	9	54	theme	mim	1247:1249	arg1	[Br					1253:1255	[C6O2(mim)2][Br]2	1241:1257	[C6O2(mim)2][Br]2	1241:1257	White hide powder/cellulose composites were successfully dissolved in [C6O2(mim)2][Br]2.
25727745	6	55	theme	common	890:895	arg1	liquids					903:909	common ionic liquids	890:909	common ionic liquids	890:909	The dissolution time of [C6O2(mim)2][Br]2 for white hide powder was shorter than those of common ionic liquids.
25727745	1	56	theme	white	389:393	arg1	composite					417:425	white hide powder/cellulose composite	389:425	white hide powder/cellulose composite	389:425	Gemini imidazolium ionic liquid, 3,3'-[1,2-ethanediylbis (oxy-2,1-ethanediyl)]-bis[1-methyl-imidazolium]-dibromide ([C6O2(mim)2][Br]2), was used for the dissolution and regeneration of white hide powder (from pigskin), and blend white hide powder with cellulose for the easy production of white hide powder/cellulose composite.
25727745	1	57	theme	-bis[1-methyl-imidazolium	178:202	arg1	-dibromide					204:213	3,3'-[1,2-ethanediylbis (oxy-2,1-ethanediyl)]-bis[1-methyl-imidazolium]-dibromide	133:213	3,3'-[1,2-ethanediylbis (oxy-2,1-ethanediyl)]-bis[1-methyl-imidazolium]-dibromide ([C6O2(mim)2][Br]2)	133:233	Gemini imidazolium ionic liquid, 3,3'-[1,2-ethanediylbis (oxy-2,1-ethanediyl)]-bis[1-methyl-imidazolium]-dibromide ([C6O2(mim)2][Br]2), was used for the dissolution and regeneration of white hide powder (from pigskin), and blend white hide powder with cellulose for the easy production of white hide powder/cellulose composite.
25727745	1	57	theme	-bis[1-methyl-imidazolium	178:202	arg1	[Br					228:230	[C6O2(mim)2][Br]2	216:232	[C6O2(mim)2][Br]2	216:232	Gemini imidazolium ionic liquid, 3,3'-[1,2-ethanediylbis (oxy-2,1-ethanediyl)]-bis[1-methyl-imidazolium]-dibromide ([C6O2(mim)2][Br]2), was used for the dissolution and regeneration of white hide powder (from pigskin), and blend white hide powder with cellulose for the easy production of white hide powder/cellulose composite.
25727745	1	57	theme	-bis[1-methyl-imidazolium	178:202	arg1	Gemini					100:105	Gemini	100:105	Gemini imidazolium ionic liquid	100:130	Gemini imidazolium ionic liquid, 3,3'-[1,2-ethanediylbis (oxy-2,1-ethanediyl)]-bis[1-methyl-imidazolium]-dibromide ([C6O2(mim)2][Br]2), was used for the dissolution and regeneration of white hide powder (from pigskin), and blend white hide powder with cellulose for the easy production of white hide powder/cellulose composite.
25727745	6	58	dep	powder	857:862	arg1	hide					852:855	hide	852:855	hide	852:855	The dissolution time of [C6O2(mim)2][Br]2 for white hide powder was shorter than those of common ionic liquids.
25727745	1	59	theme	powder/cellulose	400:415	arg1	composite					417:425	white hide powder/cellulose composite	389:425	white hide powder/cellulose composite	389:425	Gemini imidazolium ionic liquid, 3,3'-[1,2-ethanediylbis (oxy-2,1-ethanediyl)]-bis[1-methyl-imidazolium]-dibromide ([C6O2(mim)2][Br]2), was used for the dissolution and regeneration of white hide powder (from pigskin), and blend white hide powder with cellulose for the easy production of white hide powder/cellulose composite.
25727745	8	60	from	dissolution	1065:1075	arg1	[C6O2					1080:1084	[C6O2	1080:1084	[C6O2(mim)	1080:1089	The possible mechanism of white hide powder dissolution in [C6O2(mim)2][Br]2 and the regeneration of white hide powder in methanol had been proposed.
25727745	8	60	from	dissolution	1065:1075	arg1	mim					1086:1088	mim	1086:1088	mim	1086:1088	The possible mechanism of white hide powder dissolution in [C6O2(mim)2][Br]2 and the regeneration of white hide powder in methanol had been proposed.
25727745	11	61	theme	powder/cellulose	1402:1417	arg1	films					1429:1433	white hide powder/cellulose composite films	1391:1433	white hide powder/cellulose composite films	1391:1433	The tensile strength, and elongation at break of white hide powder/cellulose composite films were tested.
25727745	1	62	theme	[C6O2	216:220	arg1	[Br					228:230	[C6O2(mim)2][Br]2	216:232	[C6O2(mim)2][Br]2	216:232	Gemini imidazolium ionic liquid, 3,3'-[1,2-ethanediylbis (oxy-2,1-ethanediyl)]-bis[1-methyl-imidazolium]-dibromide ([C6O2(mim)2][Br]2), was used for the dissolution and regeneration of white hide powder (from pigskin), and blend white hide powder with cellulose for the easy production of white hide powder/cellulose composite.
25727745	1	62	theme	[C6O2	216:220	arg1	-dibromide					204:213	3,3'-[1,2-ethanediylbis (oxy-2,1-ethanediyl)]-bis[1-methyl-imidazolium]-dibromide	133:213	3,3'-[1,2-ethanediylbis (oxy-2,1-ethanediyl)]-bis[1-methyl-imidazolium]-dibromide ([C6O2(mim)2][Br]2)	133:233	Gemini imidazolium ionic liquid, 3,3'-[1,2-ethanediylbis (oxy-2,1-ethanediyl)]-bis[1-methyl-imidazolium]-dibromide ([C6O2(mim)2][Br]2), was used for the dissolution and regeneration of white hide powder (from pigskin), and blend white hide powder with cellulose for the easy production of white hide powder/cellulose composite.
25727745	1	63	dep	[Br	228:230	arg1	2					226:226	2	226:226	2	226:226	Gemini imidazolium ionic liquid, 3,3'-[1,2-ethanediylbis (oxy-2,1-ethanediyl)]-bis[1-methyl-imidazolium]-dibromide ([C6O2(mim)2][Br]2), was used for the dissolution and regeneration of white hide powder (from pigskin), and blend white hide powder with cellulose for the easy production of white hide powder/cellulose composite.
25727745	11	64	theme	white	1391:1395	arg1	films					1429:1433	white hide powder/cellulose composite films	1391:1433	white hide powder/cellulose composite films	1391:1433	The tensile strength, and elongation at break of white hide powder/cellulose composite films were tested.
25727745	1	65	theme	white	329:333	arg1	powder					340:345	blend white hide powder	323:345	blend white hide powder	323:345	Gemini imidazolium ionic liquid, 3,3'-[1,2-ethanediylbis (oxy-2,1-ethanediyl)]-bis[1-methyl-imidazolium]-dibromide ([C6O2(mim)2][Br]2), was used for the dissolution and regeneration of white hide powder (from pigskin), and blend white hide powder with cellulose for the easy production of white hide powder/cellulose composite.
25727745	1	66	theme	mim	222:224	arg1	[Br					228:230	[C6O2(mim)2][Br]2	216:232	[C6O2(mim)2][Br]2	216:232	Gemini imidazolium ionic liquid, 3,3'-[1,2-ethanediylbis (oxy-2,1-ethanediyl)]-bis[1-methyl-imidazolium]-dibromide ([C6O2(mim)2][Br]2), was used for the dissolution and regeneration of white hide powder (from pigskin), and blend white hide powder with cellulose for the easy production of white hide powder/cellulose composite.
25727745	1	66	theme	mim	222:224	arg1	-dibromide					204:213	3,3'-[1,2-ethanediylbis (oxy-2,1-ethanediyl)]-bis[1-methyl-imidazolium]-dibromide	133:213	3,3'-[1,2-ethanediylbis (oxy-2,1-ethanediyl)]-bis[1-methyl-imidazolium]-dibromide ([C6O2(mim)2][Br]2)	133:233	Gemini imidazolium ionic liquid, 3,3'-[1,2-ethanediylbis (oxy-2,1-ethanediyl)]-bis[1-methyl-imidazolium]-dibromide ([C6O2(mim)2][Br]2), was used for the dissolution and regeneration of white hide powder (from pigskin), and blend white hide powder with cellulose for the easy production of white hide powder/cellulose composite.
25727745	11	67	from	break	1382:1386	arg1	strength					1354:1361	tensile strength	1346:1361	tensile strength	1346:1361	The tensile strength, and elongation at break of white hide powder/cellulose composite films were tested.
25727745	11	67	from	break	1382:1386	arg1	elongation					1368:1377	elongation	1368:1377	elongation	1368:1377	The tensile strength, and elongation at break of white hide powder/cellulose composite films were tested.
25727745	5	68	dep	powder	776:781	arg1	hide					771:774	hide	771:774	hide	771:774	The dissolution time was 55 min when the white hide powder was 8% at 120°C.
25727745	1	69	theme	powder	340:345	arg1	regeneration					269:280	regeneration	269:280	regeneration	269:280	Gemini imidazolium ionic liquid, 3,3'-[1,2-ethanediylbis (oxy-2,1-ethanediyl)]-bis[1-methyl-imidazolium]-dibromide ([C6O2(mim)2][Br]2), was used for the dissolution and regeneration of white hide powder (from pigskin), and blend white hide powder with cellulose for the easy production of white hide powder/cellulose composite.
25727745	1	69	theme	powder	340:345	arg1	dissolution					253:263	dissolution	253:263	dissolution	253:263	Gemini imidazolium ionic liquid, 3,3'-[1,2-ethanediylbis (oxy-2,1-ethanediyl)]-bis[1-methyl-imidazolium]-dibromide ([C6O2(mim)2][Br]2), was used for the dissolution and regeneration of white hide powder (from pigskin), and blend white hide powder with cellulose for the easy production of white hide powder/cellulose composite.
25727745	1	70	theme	liquid	125:130	arg1	-dibromide					204:213	3,3'-[1,2-ethanediylbis (oxy-2,1-ethanediyl)]-bis[1-methyl-imidazolium]-dibromide	133:213	3,3'-[1,2-ethanediylbis (oxy-2,1-ethanediyl)]-bis[1-methyl-imidazolium]-dibromide ([C6O2(mim)2][Br]2)	133:233	Gemini imidazolium ionic liquid, 3,3'-[1,2-ethanediylbis (oxy-2,1-ethanediyl)]-bis[1-methyl-imidazolium]-dibromide ([C6O2(mim)2][Br]2), was used for the dissolution and regeneration of white hide powder (from pigskin), and blend white hide powder with cellulose for the easy production of white hide powder/cellulose composite.
25727745	1	70	theme	liquid	125:130	arg1	Gemini					100:105	Gemini	100:105	Gemini imidazolium ionic liquid	100:130	Gemini imidazolium ionic liquid, 3,3'-[1,2-ethanediylbis (oxy-2,1-ethanediyl)]-bis[1-methyl-imidazolium]-dibromide ([C6O2(mim)2][Br]2), was used for the dissolution and regeneration of white hide powder (from pigskin), and blend white hide powder with cellulose for the easy production of white hide powder/cellulose composite.
25727745	4	71	theme	good	689:692	arg1	[Br					677:679	[C6O2(mim)2][Br]2	665:681	[C6O2(mim)2][Br]2	665:681	The results showed that [C6O2(mim)2][Br]2 was a good solvent to white hide powder.
25727745	4	71	theme	good	689:692	arg1	solvent					694:700	a good solvent	687:700	a good solvent to white hide powder	687:721	The results showed that [C6O2(mim)2][Br]2 was a good solvent to white hide powder.
25727745	8	72	theme	regeneration	1106:1117	arg1	mechanism					1034:1042	The possible mechanism	1021:1042	The possible mechanism of white hide powder dissolution in [C6O2(mim)2][Br]2 and the regeneration of white hide powder in methanol	1021:1150	The possible mechanism of white hide powder dissolution in [C6O2(mim)2][Br]2 and the regeneration of white hide powder in methanol had been proposed.
25727745	9	73	dep	composites	1199:1208	arg1	hide					1177:1180	hide	1177:1180	hide	1177:1180	White hide powder/cellulose composites were successfully dissolved in [C6O2(mim)2][Br]2.
25727745	11	74	theme	films	1429:1433	arg1	strength					1354:1361	tensile strength	1346:1361	tensile strength	1346:1361	The tensile strength, and elongation at break of white hide powder/cellulose composite films were tested.
25727745	11	74	theme	films	1429:1433	arg1	elongation					1368:1377	elongation	1368:1377	elongation	1368:1377	The tensile strength, and elongation at break of white hide powder/cellulose composite films were tested.
25727745	1	75	theme	3,3'-	133:137	arg1	-dibromide					204:213	3,3'-[1,2-ethanediylbis (oxy-2,1-ethanediyl)]-bis[1-methyl-imidazolium]-dibromide	133:213	3,3'-[1,2-ethanediylbis (oxy-2,1-ethanediyl)]-bis[1-methyl-imidazolium]-dibromide ([C6O2(mim)2][Br]2)	133:233	Gemini imidazolium ionic liquid, 3,3'-[1,2-ethanediylbis (oxy-2,1-ethanediyl)]-bis[1-methyl-imidazolium]-dibromide ([C6O2(mim)2][Br]2), was used for the dissolution and regeneration of white hide powder (from pigskin), and blend white hide powder with cellulose for the easy production of white hide powder/cellulose composite.
25727745	1	75	theme	3,3'-	133:137	arg1	[Br					228:230	[C6O2(mim)2][Br]2	216:232	[C6O2(mim)2][Br]2	216:232	Gemini imidazolium ionic liquid, 3,3'-[1,2-ethanediylbis (oxy-2,1-ethanediyl)]-bis[1-methyl-imidazolium]-dibromide ([C6O2(mim)2][Br]2), was used for the dissolution and regeneration of white hide powder (from pigskin), and blend white hide powder with cellulose for the easy production of white hide powder/cellulose composite.
25727745	1	75	theme	3,3'-	133:137	arg1	Gemini					100:105	Gemini	100:105	Gemini imidazolium ionic liquid	100:130	Gemini imidazolium ionic liquid, 3,3'-[1,2-ethanediylbis (oxy-2,1-ethanediyl)]-bis[1-methyl-imidazolium]-dibromide ([C6O2(mim)2][Br]2), was used for the dissolution and regeneration of white hide powder (from pigskin), and blend white hide powder with cellulose for the easy production of white hide powder/cellulose composite.
25727745	8	76	theme	white	1122:1126	arg1	powder					1133:1138	white hide powder	1122:1138	white hide powder in methanol	1122:1150	The possible mechanism of white hide powder dissolution in [C6O2(mim)2][Br]2 and the regeneration of white hide powder in methanol had been proposed.
25727745	4	77	dep	white	705:709	arg1	hide					711:714	hide	711:714	hide	711:714	The results showed that [C6O2(mim)2][Br]2 was a good solvent to white hide powder.
25727745	3	78	theme	native	511:516	arg1	[Br					552:554	The native white hide powder and [C6O2(mim)2][Br]2	507:556	The native white hide powder and [C6O2(mim)2][Br]2 regenerated white hide powder	507:586	The native white hide powder and [C6O2(mim)2][Br]2 regenerated white hide powder were characterized by FT-IR, XRD, DSC-TG and FE-SEM.
25727745	8	79	theme	powder	1133:1138	arg1	regeneration					1106:1117	the regeneration	1102:1117	the regeneration of white hide powder in methanol	1102:1150	The possible mechanism of white hide powder dissolution in [C6O2(mim)2][Br]2 and the regeneration of white hide powder in methanol had been proposed.
25727745	8	79	theme	powder	1133:1138	arg1	[Br					1092:1094	white hide powder dissolution in [C6O2(mim)2][Br]2	1047:1096	white hide powder dissolution in [C6O2(mim)2][Br]2	1047:1096	The possible mechanism of white hide powder dissolution in [C6O2(mim)2][Br]2 and the regeneration of white hide powder in methanol had been proposed.
25727745	11	80	theme	tensile	1346:1352	arg1	strength					1354:1361	tensile strength	1346:1361	tensile strength	1346:1361	The tensile strength, and elongation at break of white hide powder/cellulose composite films were tested.
25727745	7	81	theme	helical	923:929	arg1	structure					931:939	The triple helical structure	912:939	The triple helical structure of white hide powder	912:960	The triple helical structure of white hide powder was partly destroyed during [C6O2(mim)2][Br]2 dissolution.
25727745	1	82	theme	powder	296:301	arg1	regeneration					269:280	regeneration	269:280	regeneration	269:280	Gemini imidazolium ionic liquid, 3,3'-[1,2-ethanediylbis (oxy-2,1-ethanediyl)]-bis[1-methyl-imidazolium]-dibromide ([C6O2(mim)2][Br]2), was used for the dissolution and regeneration of white hide powder (from pigskin), and blend white hide powder with cellulose for the easy production of white hide powder/cellulose composite.
25727745	1	82	theme	powder	296:301	arg1	dissolution					253:263	dissolution	253:263	dissolution	253:263	Gemini imidazolium ionic liquid, 3,3'-[1,2-ethanediylbis (oxy-2,1-ethanediyl)]-bis[1-methyl-imidazolium]-dibromide ([C6O2(mim)2][Br]2), was used for the dissolution and regeneration of white hide powder (from pigskin), and blend white hide powder with cellulose for the easy production of white hide powder/cellulose composite.
25727745	2	83	theme	Dissolution	428:438	arg1	performance					440:450	Dissolution performance	428:450	Dissolution performance of white hide powder in [C6O2(mim)2][Br]2	428:492	Dissolution performance of white hide powder in [C6O2(mim)2][Br]2 was studied.
25727745	2	84	from	performance	440:450	arg1	[Br					488:490	[C6O2(mim)2][Br	476:490	[C6O2(mim)2][Br	476:490	Dissolution performance of white hide powder in [C6O2(mim)2][Br]2 was studied.
25727745	12	85	theme	tissue	1561:1566	arg1	engineering					1568:1578	collagen-based tissue engineering	1546:1578	collagen-based tissue engineering	1546:1578	This work demonstrated that the white hide powder/cellulose composite exhibited some potential in collagen-based tissue engineering.
25727745	9	86	theme	White	1171:1175	arg1	composites					1199:1208	White hide powder/cellulose composites	1171:1208	White hide powder/cellulose composites	1171:1208	White hide powder/cellulose composites were successfully dissolved in [C6O2(mim)2][Br]2.
25727745	8	87	from	regeneration	1106:1117	arg1	methanol					1143:1150	methanol	1143:1150	methanol	1143:1150	The possible mechanism of white hide powder dissolution in [C6O2(mim)2][Br]2 and the regeneration of white hide powder in methanol had been proposed.
25727745	9	88	theme	powder/cellulose	1182:1197	arg1	composites					1199:1208	White hide powder/cellulose composites	1171:1208	White hide powder/cellulose composites	1171:1208	White hide powder/cellulose composites were successfully dissolved in [C6O2(mim)2][Br]2.
25727745	8	89	theme	possible	1025:1032	arg1	mechanism					1034:1042	The possible mechanism	1021:1042	The possible mechanism of white hide powder dissolution in [C6O2(mim)2][Br]2 and the regeneration of white hide powder in methanol	1021:1150	The possible mechanism of white hide powder dissolution in [C6O2(mim)2][Br]2 and the regeneration of white hide powder in methanol had been proposed.
25727745	4	90	dep	[Br	677:679	arg1	2					675:675	2	675:675	2	675:675	The results showed that [C6O2(mim)2][Br]2 was a good solvent to white hide powder.
25727745	5	91	theme	dissolution	728:738	arg1	time					740:743	The dissolution time	724:743	The dissolution time	724:743	The dissolution time was 55 min when the white hide powder was 8% at 120°C.
25727745	5	91	theme	dissolution	728:738	arg1	min					752:754	55 min	749:754	55 min	749:754	The dissolution time was 55 min when the white hide powder was 8% at 120°C.
25727745	7	92	theme	[Br	1002:1004	arg1	dissolution					1008:1018	[C6O2(mim)2][Br]2 dissolution	990:1018	[C6O2(mim)2][Br]2 dissolution	990:1018	The triple helical structure of white hide powder was partly destroyed during [C6O2(mim)2][Br]2 dissolution.
25727745	1	93	from	pigskin	309:315	arg1	regeneration					269:280	regeneration	269:280	regeneration	269:280	Gemini imidazolium ionic liquid, 3,3'-[1,2-ethanediylbis (oxy-2,1-ethanediyl)]-bis[1-methyl-imidazolium]-dibromide ([C6O2(mim)2][Br]2), was used for the dissolution and regeneration of white hide powder (from pigskin), and blend white hide powder with cellulose for the easy production of white hide powder/cellulose composite.
25727745	1	93	from	pigskin	309:315	arg1	dissolution					253:263	dissolution	253:263	dissolution	253:263	Gemini imidazolium ionic liquid, 3,3'-[1,2-ethanediylbis (oxy-2,1-ethanediyl)]-bis[1-methyl-imidazolium]-dibromide ([C6O2(mim)2][Br]2), was used for the dissolution and regeneration of white hide powder (from pigskin), and blend white hide powder with cellulose for the easy production of white hide powder/cellulose composite.
25727745	1	93	from	pigskin	309:315	arg1	powder					340:345	blend white hide powder	323:345	blend white hide powder	323:345	Gemini imidazolium ionic liquid, 3,3'-[1,2-ethanediylbis (oxy-2,1-ethanediyl)]-bis[1-methyl-imidazolium]-dibromide ([C6O2(mim)2][Br]2), was used for the dissolution and regeneration of white hide powder (from pigskin), and blend white hide powder with cellulose for the easy production of white hide powder/cellulose composite.
25727745	1	93	from	pigskin	309:315	arg1	powder					296:301	powder	296:301	powder (from pigskin)	296:316	Gemini imidazolium ionic liquid, 3,3'-[1,2-ethanediylbis (oxy-2,1-ethanediyl)]-bis[1-methyl-imidazolium]-dibromide ([C6O2(mim)2][Br]2), was used for the dissolution and regeneration of white hide powder (from pigskin), and blend white hide powder with cellulose for the easy production of white hide powder/cellulose composite.
25727745	2	94	dep	powder	466:471	arg1	hide					461:464	hide	461:464	hide	461:464	Dissolution performance of white hide powder in [C6O2(mim)2][Br]2 was studied.
25727745	10	95	theme	hide	1285:1288	arg1	film					1307:1310	white hide powder/cellulose film	1279:1310	white hide powder/cellulose film	1279:1310	The performance of white hide powder/cellulose film was measured by FT-IR and TG.
25727745	0	96	theme	powder/cellulose	37:52	arg1	regeneration					16:27	regeneration	16:27	regeneration	16:27	Dissolution and regeneration of hide powder/cellulose composite in Gemini imidazolium ionic liquid.
25727745	0	96	theme	powder/cellulose	37:52	arg1	Dissolution					0:10	Dissolution	0:10	Dissolution	0:10	Dissolution and regeneration of hide powder/cellulose composite in Gemini imidazolium ionic liquid.
25727745	12	97	theme	hide	1486:1489	arg1	composite					1508:1516	the white hide powder/cellulose composite	1476:1516	the white hide powder/cellulose composite	1476:1516	This work demonstrated that the white hide powder/cellulose composite exhibited some potential in collagen-based tissue engineering.
25727745	9	98	theme	[C6O2	1241:1245	arg1	[Br					1253:1255	[C6O2(mim)2][Br]2	1241:1257	[C6O2(mim)2][Br]2	1241:1257	White hide powder/cellulose composites were successfully dissolved in [C6O2(mim)2][Br]2.
25727745	7	99	theme	mim	996:998	arg1	dissolution					1008:1018	[C6O2(mim)2][Br]2 dissolution	990:1018	[C6O2(mim)2][Br]2 dissolution	990:1018	The triple helical structure of white hide powder was partly destroyed during [C6O2(mim)2][Br]2 dissolution.
25727745	3	100	dep	powder	581:586	arg1	hide					576:579	hide	576:579	hide	576:579	The native white hide powder and [C6O2(mim)2][Br]2 regenerated white hide powder were characterized by FT-IR, XRD, DSC-TG and FE-SEM.
25727745	0	101	dep	powder/cellulose	37:52	arg1	hide					32:35	hide	32:35	hide	32:35	Dissolution and regeneration of hide powder/cellulose composite in Gemini imidazolium ionic liquid.
25727745	7	102	theme	[C6O2	990:994	arg1	dissolution					1008:1018	[C6O2(mim)2][Br]2 dissolution	990:1018	[C6O2(mim)2][Br]2 dissolution	990:1018	The triple helical structure of white hide powder was partly destroyed during [C6O2(mim)2][Br]2 dissolution.
25727745	6	103	dep	[Br	836:838	arg1	2					834:834	2	834:834	2	834:834	The dissolution time of [C6O2(mim)2][Br]2 for white hide powder was shorter than those of common ionic liquids.
25727745	8	104	dep	[Br	1092:1094	arg1	2					1090:1090	2	1090:1090	2	1090:1090	The possible mechanism of white hide powder dissolution in [C6O2(mim)2][Br]2 and the regeneration of white hide powder in methanol had been proposed.
25727745	8	104	dep	[Br	1092:1094	arg1	hide					1053:1056	hide	1053:1056	hide powder dissolution in [C6O2(mim)	1053:1089	The possible mechanism of white hide powder dissolution in [C6O2(mim)2][Br]2 and the regeneration of white hide powder in methanol had been proposed.
25727745	10	105	theme	film	1307:1310	arg1	performance					1264:1274	The performance	1260:1274	The performance of white hide powder/cellulose film	1260:1310	The performance of white hide powder/cellulose film was measured by FT-IR and TG.
25727745	2	106	dep	[Br	488:490	arg1	2					486:486	2	486:486	2	486:486	Dissolution performance of white hide powder in [C6O2(mim)2][Br]2 was studied.
25727745	3	107	theme	regenerated	558:568	arg1	powder					581:586	regenerated white hide powder	558:586	The native white hide powder and [C6O2(mim)2][Br]2 regenerated white hide powder	507:586	The native white hide powder and [C6O2(mim)2][Br]2 regenerated white hide powder were characterized by FT-IR, XRD, DSC-TG and FE-SEM.
25727745	8	108	from	methanol	1143:1150	arg1	regeneration					1106:1117	the regeneration	1102:1117	the regeneration of white hide powder in methanol	1102:1150	The possible mechanism of white hide powder dissolution in [C6O2(mim)2][Br]2 and the regeneration of white hide powder in methanol had been proposed.
25727745	8	108	from	methanol	1143:1150	arg1	[Br					1092:1094	white hide powder dissolution in [C6O2(mim)2][Br]2	1047:1096	white hide powder dissolution in [C6O2(mim)2][Br]2	1047:1096	The possible mechanism of white hide powder dissolution in [C6O2(mim)2][Br]2 and the regeneration of white hide powder in methanol had been proposed.
24632517	9	0	theme	optimum	1218:1224	arg1	source					1233:1238	the optimum carbon source	1214:1238	the optimum carbon source	1214:1238	Glucose was found to be the optimum carbon source.
24632517	9	0	theme	optimum	1218:1224	arg1	Glucose					1190:1196	Glucose	1190:1196	Glucose	1190:1196	Glucose was found to be the optimum carbon source.
24632517	7	1	theme	acid	935:938	arg1	hydrolysate					940:950	casein acid hydrolysate	928:950	casein acid hydrolysate	928:950	Both beef extract and casein acid hydrolysate required chemical pre-treatment to remove polysaccharide components before the medium was free of EPS-E materials.
24632517	0	2	theme	structural	77:86	arg1	characterisation					88:103	structural characterisation	77:103	structural characterisation	77:103	Development of a growth medium suitable for exopolysaccharide production and structural characterisation by Bifidobacterium animalis ssp.
24632517	9	3	theme	carbon	1226:1231	arg1	source					1233:1238	the optimum carbon source	1214:1238	the optimum carbon source	1214:1238	Glucose was found to be the optimum carbon source.
24632517	9	3	theme	carbon	1226:1231	arg1	Glucose					1190:1196	Glucose	1190:1196	Glucose	1190:1196	Glucose was found to be the optimum carbon source.
24632517	0	4	theme	Bifidobacterium	108:122	arg1	ssp					133:135	Bifidobacterium animalis ssp	108:135	Bifidobacterium animalis ssp	108:135	Development of a growth medium suitable for exopolysaccharide production and structural characterisation by Bifidobacterium animalis ssp.
24632517	2	5	theme	dairy	323:327	arg1	products					329:336	dairy products	323:336	dairy products	323:336	Exopolysaccharides (EPSs) produced by Bifidobacteria have received considerable attention due to their ability to modify the rheological and physicochemical properties of dairy products.
24632517	10	6	theme	medium	1251:1256	arg1	composition					1258:1268	The final medium composition	1241:1268	The final medium composition	1241:1268	The final medium composition supported growth to the same extent as RCM providing significant EPS yields and no interferences during analysis.
24632517	10	7	theme	same	1294:1297	arg1	extent					1299:1304	the same extent	1290:1304	the same extent	1290:1304	The final medium composition supported growth to the same extent as RCM providing significant EPS yields and no interferences during analysis.
24632517	10	8	theme	final	1245:1249	arg1	composition					1258:1268	The final medium composition	1241:1268	The final medium composition	1241:1268	The final medium composition supported growth to the same extent as RCM providing significant EPS yields and no interferences during analysis.
24632517	5	9	dep	quantification	695:708	arg1	the					691:693	the	691:693	the	691:693	lactis AD011 and does not interfere with the quantification and characterisation of the EPS generated.
24632517	7	10	theme	beef	911:914	arg1	extract					916:922	beef extract	911:922	beef extract	911:922	Both beef extract and casein acid hydrolysate required chemical pre-treatment to remove polysaccharide components before the medium was free of EPS-E materials.
24632517	0	11	theme	animalis	124:131	arg1	ssp					133:135	Bifidobacterium animalis ssp	108:135	Bifidobacterium animalis ssp	108:135	Development of a growth medium suitable for exopolysaccharide production and structural characterisation by Bifidobacterium animalis ssp.
24632517	5	12	theme	lactis	650:655	arg1	AD011					657:661	lactis AD011	650:661	lactis AD011	650:661	lactis AD011 and does not interfere with the quantification and characterisation of the EPS generated.
24632517	1	13	dep	AD011	145:149	arg1	lactis					138:143	lactis	138:143	lactis	138:143	lactis AD011.
24632517	2	14	theme	physicochemical	293:307	arg1	properties					309:318	the rheological and physicochemical properties	273:318	properties	309:318	Exopolysaccharides (EPSs) produced by Bifidobacteria have received considerable attention due to their ability to modify the rheological and physicochemical properties of dairy products.
24632517	10	15	theme	EPS	1335:1337	arg1	yields					1339:1344	significant EPS yields	1323:1344	significant EPS yields	1323:1344	The final medium composition supported growth to the same extent as RCM providing significant EPS yields and no interferences during analysis.
24632517	3	16	theme	complex	480:486	arg1	Medium					525:530	Reinforced Clostridial Medium	502:530	Reinforced Clostridial Medium (RCM)	502:536	However, the quantification and characterisation of Bifidobacterial EPS are hampered by the presence of EPS-equivalent (EPS-E) substances in complex media such as Reinforced Clostridial Medium (RCM).
24632517	3	16	theme	complex	480:486	arg1	media					488:492	complex media	480:492	complex media such as Reinforced Clostridial Medium (RCM)	480:536	However, the quantification and characterisation of Bifidobacterial EPS are hampered by the presence of EPS-equivalent (EPS-E) substances in complex media such as Reinforced Clostridial Medium (RCM).
24632517	3	17	from	presence	431:438	arg1	Medium					525:530	Reinforced Clostridial Medium	502:530	Reinforced Clostridial Medium (RCM)	502:536	However, the quantification and characterisation of Bifidobacterial EPS are hampered by the presence of EPS-equivalent (EPS-E) substances in complex media such as Reinforced Clostridial Medium (RCM).
24632517	3	17	from	presence	431:438	arg1	media					488:492	complex media	480:492	complex media such as Reinforced Clostridial Medium (RCM)	480:536	However, the quantification and characterisation of Bifidobacterial EPS are hampered by the presence of EPS-equivalent (EPS-E) substances in complex media such as Reinforced Clostridial Medium (RCM).
24632517	10	18	theme	significant	1323:1333	arg1	yields					1339:1344	significant EPS yields	1323:1344	significant EPS yields	1323:1344	The final medium composition supported growth to the same extent as RCM providing significant EPS yields and no interferences during analysis.
24632517	6	19	theme	components	820:829	arg1	identification					785:798	the identification	781:798	the identification of EPE-E containing components	781:829	Medium development involved the identification of EPE-E containing components via NMR analysis followed by their removal, substitution or pre-treatment.
24632517	3	20	theme	substances	466:475	arg1	presence					431:438	the presence	427:438	the presence of EPS-equivalent (EPS-E) substances in complex media such as Reinforced Clostridial Medium (RCM)	427:536	However, the quantification and characterisation of Bifidobacterial EPS are hampered by the presence of EPS-equivalent (EPS-E) substances in complex media such as Reinforced Clostridial Medium (RCM).
24632517	0	21	theme	growth	17:22	arg1	medium					24:29	a growth medium	15:29	a growth medium suitable for exopolysaccharide production and structural characterisation by Bifidobacterium animalis ssp	15:135	Development of a growth medium suitable for exopolysaccharide production and structural characterisation by Bifidobacterium animalis ssp.
24632517	8	22	theme	free	1078:1081	arg1	components					1083:1092	EPS-E free components	1072:1092	EPS-E free components	1072:1092	Once EPS-E free components had been identified, lactose, glucose and galactose were evaluated as potential carbon sources.
24632517	6	23	theme	containing	809:818	arg1	components					820:829	EPE-E containing components	803:829	EPE-E containing components	803:829	Medium development involved the identification of EPE-E containing components via NMR analysis followed by their removal, substitution or pre-treatment.
24632517	4	24	from	based	573:577	arg1	RCM					582:584	RCM	582:584	RCM	582:584	This study has developed a medium based on RCM which both supports the growth of Bifidobacterium animalis ssp.
24632517	3	25	dep	quantification	352:365	arg1	the					348:350	the	348:350	the	348:350	However, the quantification and characterisation of Bifidobacterial EPS are hampered by the presence of EPS-equivalent (EPS-E) substances in complex media such as Reinforced Clostridial Medium (RCM).
24632517	2	26	theme	products	329:336	arg1	properties					309:318	the rheological and physicochemical properties	273:318	properties	309:318	Exopolysaccharides (EPSs) produced by Bifidobacteria have received considerable attention due to their ability to modify the rheological and physicochemical properties of dairy products.
24632517	3	27	theme	Reinforced	502:511	arg1	RCM					533:535	RCM	533:535	RCM	533:535	However, the quantification and characterisation of Bifidobacterial EPS are hampered by the presence of EPS-equivalent (EPS-E) substances in complex media such as Reinforced Clostridial Medium (RCM).
24632517	3	27	theme	Reinforced	502:511	arg1	Medium					525:530	Reinforced Clostridial Medium	502:530	Reinforced Clostridial Medium (RCM)	502:536	However, the quantification and characterisation of Bifidobacterial EPS are hampered by the presence of EPS-equivalent (EPS-E) substances in complex media such as Reinforced Clostridial Medium (RCM).
24632517	0	28	theme	suitable	31:38	arg1	medium					24:29	a growth medium	15:29	a growth medium suitable for exopolysaccharide production and structural characterisation by Bifidobacterium animalis ssp	15:135	Development of a growth medium suitable for exopolysaccharide production and structural characterisation by Bifidobacterium animalis ssp.
24632517	5	29	theme	EPS	738:740	arg1	characterisation					714:729	characterisation	714:729	characterisation	714:729	lactis AD011 and does not interfere with the quantification and characterisation of the EPS generated.
24632517	5	29	theme	EPS	738:740	arg1	quantification					695:708	quantification	695:708	quantification	695:708	lactis AD011 and does not interfere with the quantification and characterisation of the EPS generated.
24632517	0	30	theme	medium	24:29	arg1	Development					0:10	Development	0:10	Development of a growth medium suitable for exopolysaccharide production and structural characterisation by Bifidobacterium animalis ssp.	0:136	Development of a growth medium suitable for exopolysaccharide production and structural characterisation by Bifidobacterium animalis ssp.
24632517	3	31	theme	EPS-E	459:463	arg1	substances					466:475	EPS-equivalent (EPS-E) substances	443:475	EPS-equivalent (EPS-E) substances in complex media such as Reinforced Clostridial Medium (RCM)	443:536	However, the quantification and characterisation of Bifidobacterial EPS are hampered by the presence of EPS-equivalent (EPS-E) substances in complex media such as Reinforced Clostridial Medium (RCM).
24632517	6	32	theme	EPE-E	803:807	arg1	components					820:829	EPE-E containing components	803:829	EPE-E containing components	803:829	Medium development involved the identification of EPE-E containing components via NMR analysis followed by their removal, substitution or pre-treatment.
24632517	8	33	theme	EPS-E	1072:1076	arg1	components					1083:1092	EPS-E free components	1072:1092	EPS-E free components	1072:1092	Once EPS-E free components had been identified, lactose, glucose and galactose were evaluated as potential carbon sources.
24632517	3	34	from	media	488:492	arg1	presence					431:438	the presence	427:438	the presence of EPS-equivalent (EPS-E) substances in complex media such as Reinforced Clostridial Medium (RCM)	427:536	However, the quantification and characterisation of Bifidobacterial EPS are hampered by the presence of EPS-equivalent (EPS-E) substances in complex media such as Reinforced Clostridial Medium (RCM).
24632517	3	35	theme	Bifidobacterial	391:405	arg1	EPS					407:409	Bifidobacterial EPS	391:409	Bifidobacterial EPS	391:409	However, the quantification and characterisation of Bifidobacterial EPS are hampered by the presence of EPS-equivalent (EPS-E) substances in complex media such as Reinforced Clostridial Medium (RCM).
24632517	0	36	theme	exopolysaccharide	44:60	arg1	production					62:71	exopolysaccharide production	44:71	exopolysaccharide production	44:71	Development of a growth medium suitable for exopolysaccharide production and structural characterisation by Bifidobacterium animalis ssp.
24632517	3	37	theme	EPS	407:409	arg1	characterisation					371:386	characterisation	371:386	characterisation	371:386	However, the quantification and characterisation of Bifidobacterial EPS are hampered by the presence of EPS-equivalent (EPS-E) substances in complex media such as Reinforced Clostridial Medium (RCM).
24632517	3	37	theme	EPS	407:409	arg1	quantification					352:365	quantification	352:365	quantification	352:365	However, the quantification and characterisation of Bifidobacterial EPS are hampered by the presence of EPS-equivalent (EPS-E) substances in complex media such as Reinforced Clostridial Medium (RCM).
24632517	8	38	theme	potential	1164:1172	arg1	lactose					1115:1121	lactose	1115:1121	lactose	1115:1121	Once EPS-E free components had been identified, lactose, glucose and galactose were evaluated as potential carbon sources.
24632517	8	38	theme	potential	1164:1172	arg1	glucose					1124:1130	glucose	1124:1130	glucose	1124:1130	Once EPS-E free components had been identified, lactose, glucose and galactose were evaluated as potential carbon sources.
24632517	8	38	theme	potential	1164:1172	arg1	galactose					1136:1144	galactose	1136:1144	galactose	1136:1144	Once EPS-E free components had been identified, lactose, glucose and galactose were evaluated as potential carbon sources.
24632517	8	38	theme	potential	1164:1172	arg1	sources					1181:1187	potential carbon sources	1164:1187	potential carbon sources	1164:1187	Once EPS-E free components had been identified, lactose, glucose and galactose were evaluated as potential carbon sources.
24632517	3	39	theme	EPS-equivalent	443:456	arg1	substances					466:475	EPS-equivalent (EPS-E) substances	443:475	EPS-equivalent (EPS-E) substances in complex media such as Reinforced Clostridial Medium (RCM)	443:536	However, the quantification and characterisation of Bifidobacterial EPS are hampered by the presence of EPS-equivalent (EPS-E) substances in complex media such as Reinforced Clostridial Medium (RCM).
24632517	4	40	theme	medium	566:571	arg1	based					573:577	a medium based	564:577	a medium based on RCM which both supports the growth of Bifidobacterium animalis ssp	564:647	This study has developed a medium based on RCM which both supports the growth of Bifidobacterium animalis ssp.
24632517	7	41	theme	polysaccharide	994:1007	arg1	components					1009:1018	polysaccharide components	994:1018	polysaccharide components	994:1018	Both beef extract and casein acid hydrolysate required chemical pre-treatment to remove polysaccharide components before the medium was free of EPS-E materials.
24632517	7	42	theme	chemical	961:968	arg1	pre-treatment					970:982	chemical pre-treatment	961:982	chemical pre-treatment	961:982	Both beef extract and casein acid hydrolysate required chemical pre-treatment to remove polysaccharide components before the medium was free of EPS-E materials.
24632517	7	43	theme	materials	1056:1064	arg1	free					1042:1045	free	1042:1045	free	1042:1045	Both beef extract and casein acid hydrolysate required chemical pre-treatment to remove polysaccharide components before the medium was free of EPS-E materials.
24632517	7	43	theme	materials	1056:1064	arg1	medium					1031:1036	the medium	1027:1036	the medium	1027:1036	Both beef extract and casein acid hydrolysate required chemical pre-treatment to remove polysaccharide components before the medium was free of EPS-E materials.
24632517	6	44	theme	NMR	835:837	arg1	analysis					839:846	NMR analysis	835:846	NMR analysis followed by their removal, substitution or pre-treatment	835:903	Medium development involved the identification of EPE-E containing components via NMR analysis followed by their removal, substitution or pre-treatment.
24632517	3	45	theme	Clostridial	513:523	arg1	RCM					533:535	RCM	533:535	RCM	533:535	However, the quantification and characterisation of Bifidobacterial EPS are hampered by the presence of EPS-equivalent (EPS-E) substances in complex media such as Reinforced Clostridial Medium (RCM).
24632517	3	45	theme	Clostridial	513:523	arg1	Medium					525:530	Reinforced Clostridial Medium	502:530	Reinforced Clostridial Medium (RCM)	502:536	However, the quantification and characterisation of Bifidobacterial EPS are hampered by the presence of EPS-equivalent (EPS-E) substances in complex media such as Reinforced Clostridial Medium (RCM).
24632517	4	46	theme	Bifidobacterium	620:634	arg1	ssp					645:647	Bifidobacterium animalis ssp	620:647	Bifidobacterium animalis ssp	620:647	This study has developed a medium based on RCM which both supports the growth of Bifidobacterium animalis ssp.
24632517	8	47	theme	carbon	1174:1179	arg1	lactose					1115:1121	lactose	1115:1121	lactose	1115:1121	Once EPS-E free components had been identified, lactose, glucose and galactose were evaluated as potential carbon sources.
24632517	8	47	theme	carbon	1174:1179	arg1	glucose					1124:1130	glucose	1124:1130	glucose	1124:1130	Once EPS-E free components had been identified, lactose, glucose and galactose were evaluated as potential carbon sources.
24632517	8	47	theme	carbon	1174:1179	arg1	galactose					1136:1144	galactose	1136:1144	galactose	1136:1144	Once EPS-E free components had been identified, lactose, glucose and galactose were evaluated as potential carbon sources.
24632517	8	47	theme	carbon	1174:1179	arg1	sources					1181:1187	potential carbon sources	1164:1187	potential carbon sources	1164:1187	Once EPS-E free components had been identified, lactose, glucose and galactose were evaluated as potential carbon sources.
24632517	2	48	theme	considerable	219:230	arg1	attention					232:240	considerable attention	219:240	considerable attention due to their ability to modify the rheological and physicochemical properties of dairy products	219:336	Exopolysaccharides (EPSs) produced by Bifidobacteria have received considerable attention due to their ability to modify the rheological and physicochemical properties of dairy products.
24632517	2	49	theme	rheological	277:287	arg1	properties					309:318	the rheological and physicochemical properties	273:318	properties	309:318	Exopolysaccharides (EPSs) produced by Bifidobacteria have received considerable attention due to their ability to modify the rheological and physicochemical properties of dairy products.
24632517	7	50	theme	EPS-E	1050:1054	arg1	materials					1056:1064	EPS-E materials	1050:1064	EPS-E materials	1050:1064	Both beef extract and casein acid hydrolysate required chemical pre-treatment to remove polysaccharide components before the medium was free of EPS-E materials.
24632517	3	51	from	substances	466:475	arg1	Medium					525:530	Reinforced Clostridial Medium	502:530	Reinforced Clostridial Medium (RCM)	502:536	However, the quantification and characterisation of Bifidobacterial EPS are hampered by the presence of EPS-equivalent (EPS-E) substances in complex media such as Reinforced Clostridial Medium (RCM).
24632517	3	51	from	substances	466:475	arg1	media					488:492	complex media	480:492	complex media such as Reinforced Clostridial Medium (RCM)	480:536	However, the quantification and characterisation of Bifidobacterial EPS are hampered by the presence of EPS-equivalent (EPS-E) substances in complex media such as Reinforced Clostridial Medium (RCM).
24632517	4	52	theme	ssp	645:647	arg1	growth					610:615	the growth	606:615	the growth of Bifidobacterium animalis ssp	606:647	This study has developed a medium based on RCM which both supports the growth of Bifidobacterium animalis ssp.
24632517	6	53	theme	Medium	753:758	arg1	development					760:770	Medium development	753:770	Medium development	753:770	Medium development involved the identification of EPE-E containing components via NMR analysis followed by their removal, substitution or pre-treatment.
24632517	7	54	theme	casein	928:933	arg1	hydrolysate					940:950	casein acid hydrolysate	928:950	casein acid hydrolysate	928:950	Both beef extract and casein acid hydrolysate required chemical pre-treatment to remove polysaccharide components before the medium was free of EPS-E materials.
24632517	4	55	theme	animalis	636:643	arg1	ssp					645:647	Bifidobacterium animalis ssp	620:647	Bifidobacterium animalis ssp	620:647	This study has developed a medium based on RCM which both supports the growth of Bifidobacterium animalis ssp.
26190688	0	0	theme	Phellinus	103:111	arg1	igniarius					113:121	Phellinus igniarius	103:121	Phellinus igniarius	103:121	Structural characterization and immunomodulatory effects of polysaccharides from Phellinus linteus and Phellinus igniarius on the IL-6/IL-10 cytokine balance of the mouse macrophage cell lines (RAW 264.7).
26190688	3	1	from	bodies	384:389	arg1	Polysaccharides					348:362	Polysaccharides	348:362	Polysaccharides from their fruiting bodies	348:389	Polysaccharides from their fruiting bodies show strong immunomodulatory activity.
26190688	2	2	theme	diseases	338:345	arg1	diseases					338:345	diseases	338:345	diseases	338:345	have been used in traditional Asian medicine for over two centuries against a variety of diseases.
26190688	2	2	theme	diseases	338:345	arg1	variety					327:333	a variety	325:333	a variety of diseases	325:345	have been used in traditional Asian medicine for over two centuries against a variety of diseases.
26190688	8	3	dep	→	1068:1068	arg1	→					1075:1075	1 → 6	1073:1077	1 → 3; 1 → 6	1066:1077	In vitro assays by RT-PCR and ELISA showed that (1 → 3; 1 → 6)-β-D-polysaccharides from P. linteus and P. igniarius decreased TNF-α in RAW 264.7 cells, suggesting an immuno-suppressive activity.
26190688	4	4	dep	structure	465:473	arg1	the					461:463	the	461:463	the	461:463	In this study we characterized the structure and composition of polysaccharides from Phellinus linteus and Phellinus igniarius by HPLC, GC-MS and NMR (1-H, 13-C, COSY, NOESY and TOCSY).
26190688	5	5	theme	xylose	740:745	arg1	proportions					705:715	minor proportions	699:715	minor proportions of mannose, galactose, xylose, arabinose and rhamnose	699:769	The polysaccharides from P. linteus and P. igniarius mainly contained glucose with minor proportions of mannose, galactose, xylose, arabinose and rhamnose.
26190688	0	6	from	characterization	11:26	arg1	linteus					91:97	Phellinus linteus	81:97	Phellinus linteus	81:97	Structural characterization and immunomodulatory effects of polysaccharides from Phellinus linteus and Phellinus igniarius on the IL-6/IL-10 cytokine balance of the mouse macrophage cell lines (RAW 264.7).
26190688	0	6	from	characterization	11:26	arg1	igniarius					113:121	Phellinus igniarius	103:121	Phellinus igniarius	103:121	Structural characterization and immunomodulatory effects of polysaccharides from Phellinus linteus and Phellinus igniarius on the IL-6/IL-10 cytokine balance of the mouse macrophage cell lines (RAW 264.7).
26190688	0	6	from	characterization	11:26	arg1	balance					150:156	the IL-6/IL-10 cytokine balance	126:156	the IL-6/IL-10 cytokine balance of the mouse macrophage cell lines (RAW 264.7)	126:203	Structural characterization and immunomodulatory effects of polysaccharides from Phellinus linteus and Phellinus igniarius on the IL-6/IL-10 cytokine balance of the mouse macrophage cell lines (RAW 264.7).
26190688	4	7	from	linteus	525:531	arg1	polysaccharides					494:508	polysaccharides	494:508	polysaccharides from Phellinus linteus and Phellinus igniarius	494:555	In this study we characterized the structure and composition of polysaccharides from Phellinus linteus and Phellinus igniarius by HPLC, GC-MS and NMR (1-H, 13-C, COSY, NOESY and TOCSY).
26190688	4	7	from	linteus	525:531	arg1	composition					479:489	composition	479:489	composition	479:489	In this study we characterized the structure and composition of polysaccharides from Phellinus linteus and Phellinus igniarius by HPLC, GC-MS and NMR (1-H, 13-C, COSY, NOESY and TOCSY).
26190688	4	7	from	linteus	525:531	arg1	structure					465:473	structure	465:473	structure	465:473	In this study we characterized the structure and composition of polysaccharides from Phellinus linteus and Phellinus igniarius by HPLC, GC-MS and NMR (1-H, 13-C, COSY, NOESY and TOCSY).
26190688	10	8	theme	chronic	1534:1540	arg1	obesity					1564:1570	obesity	1564:1570	obesity	1564:1570	The results suggest that polysaccharides from P. linteus and P. igniarius could possibly find applications in restoring the IL-6/IL-10 balance, the disturbance of which is thought to be related to chronic inflammatory disease, obesity, diabetes type 2, and to mania and depression.
26190688	10	8	theme	chronic	1534:1540	arg1	type					1582:1585	diabetes type 2	1573:1587	diabetes type 2	1573:1587	The results suggest that polysaccharides from P. linteus and P. igniarius could possibly find applications in restoring the IL-6/IL-10 balance, the disturbance of which is thought to be related to chronic inflammatory disease, obesity, diabetes type 2, and to mania and depression.
26190688	10	8	theme	chronic	1534:1540	arg1	disease					1555:1561	chronic inflammatory disease	1534:1561	chronic inflammatory disease	1534:1561	The results suggest that polysaccharides from P. linteus and P. igniarius could possibly find applications in restoring the IL-6/IL-10 balance, the disturbance of which is thought to be related to chronic inflammatory disease, obesity, diabetes type 2, and to mania and depression.
26190688	7	9	theme	side	1005:1008	arg1	chain					1010:1014	1→ side chain	1002:1014	→6)-β-D-Glcp-(1→ side chain	988:1014	The two-dimensional COSY, NOESY and TOCSY confirmed that these polysaccharides have a main chain of →3)-β-D-Glcp-(1→ with →6)-β-D-Glcp-(1→ side chain.
26190688	5	10	theme	minor	699:703	arg1	proportions					705:715	minor proportions	699:715	minor proportions of mannose, galactose, xylose, arabinose and rhamnose	699:769	The polysaccharides from P. linteus and P. igniarius mainly contained glucose with minor proportions of mannose, galactose, xylose, arabinose and rhamnose.
26190688	7	11	theme	1→	1002:1003	arg1	chain					1010:1014	1→ side chain	1002:1014	→6)-β-D-Glcp-(1→ side chain	988:1014	The two-dimensional COSY, NOESY and TOCSY confirmed that these polysaccharides have a main chain of →3)-β-D-Glcp-(1→ with →6)-β-D-Glcp-(1→ side chain.
26190688	8	12	from	TNF-α	1143:1147	arg1	cells					1162:1166	RAW 264.7 cells	1152:1166	RAW 264.7 cells	1152:1166	In vitro assays by RT-PCR and ELISA showed that (1 → 3; 1 → 6)-β-D-polysaccharides from P. linteus and P. igniarius decreased TNF-α in RAW 264.7 cells, suggesting an immuno-suppressive activity.
26190688	2	13	theme	Asian	279:283	arg1	medicine					285:292	traditional Asian medicine	267:292	traditional Asian medicine	267:292	have been used in traditional Asian medicine for over two centuries against a variety of diseases.
26190688	10	14	from	linteus	1386:1392	arg1	polysaccharides					1362:1376	polysaccharides	1362:1376	polysaccharides from P. linteus and P. igniarius	1362:1409	The results suggest that polysaccharides from P. linteus and P. igniarius could possibly find applications in restoring the IL-6/IL-10 balance, the disturbance of which is thought to be related to chronic inflammatory disease, obesity, diabetes type 2, and to mania and depression.
26190688	5	15	theme	P.	641:642	arg1	linteus					644:650	P. linteus	641:650	P. linteus	641:650	The polysaccharides from P. linteus and P. igniarius mainly contained glucose with minor proportions of mannose, galactose, xylose, arabinose and rhamnose.
26190688	6	16	theme	Methylation	772:782	arg1	analyses					784:791	Methylation analyses	772:791	Methylation analyses	772:791	Methylation analyses showed that the glycosidic linkages were mostly 1 → 3, 1 → 6 or 1 → 3,6.
26190688	2	17	theme	traditional	267:277	arg1	medicine					285:292	traditional Asian medicine	267:292	traditional Asian medicine	267:292	have been used in traditional Asian medicine for over two centuries against a variety of diseases.
26190688	8	18	theme	In	1017:1018	arg1	assays					1026:1031	In vitro assays	1017:1031	In vitro assays by RT-PCR and ELISA	1017:1051	In vitro assays by RT-PCR and ELISA showed that (1 → 3; 1 → 6)-β-D-polysaccharides from P. linteus and P. igniarius decreased TNF-α in RAW 264.7 cells, suggesting an immuno-suppressive activity.
26190688	0	19	theme	IL-6/IL-10	130:139	arg1	balance					150:156	the IL-6/IL-10 cytokine balance	126:156	the IL-6/IL-10 cytokine balance of the mouse macrophage cell lines (RAW 264.7)	126:203	Structural characterization and immunomodulatory effects of polysaccharides from Phellinus linteus and Phellinus igniarius on the IL-6/IL-10 cytokine balance of the mouse macrophage cell lines (RAW 264.7).
26190688	7	20	with	chain	957:961	arg1	-β-D-Glcp-					991:1000	→6)-β-D-Glcp-(1→ side chain	988:1014	→6)-β-D-Glcp-(1→ side chain	988:1014	The two-dimensional COSY, NOESY and TOCSY confirmed that these polysaccharides have a main chain of →3)-β-D-Glcp-(1→ with →6)-β-D-Glcp-(1→ side chain.
26190688	7	21	contain	have	945:948	arg2	chain					957:961	a main chain	950:961	a main chain of →3)-β-D-Glcp-(1→ with →6)-β-D-Glcp-(1→ side chain	950:1014	The two-dimensional COSY, NOESY and TOCSY confirmed that these polysaccharides have a main chain of →3)-β-D-Glcp-(1→ with →6)-β-D-Glcp-(1→ side chain.
26190688	7	21	contain	have	945:948	arg1	polysaccharides					929:943	these polysaccharides	923:943	these polysaccharides	923:943	The two-dimensional COSY, NOESY and TOCSY confirmed that these polysaccharides have a main chain of →3)-β-D-Glcp-(1→ with →6)-β-D-Glcp-(1→ side chain.
26190688	3	22	theme	strong	396:401	arg1	activity					420:427	strong immunomodulatory activity	396:427	strong immunomodulatory activity	396:427	Polysaccharides from their fruiting bodies show strong immunomodulatory activity.
26190688	4	23	theme	polysaccharides	494:508	arg1	composition					479:489	composition	479:489	composition	479:489	In this study we characterized the structure and composition of polysaccharides from Phellinus linteus and Phellinus igniarius by HPLC, GC-MS and NMR (1-H, 13-C, COSY, NOESY and TOCSY).
26190688	4	23	theme	polysaccharides	494:508	arg1	structure					465:473	structure	465:473	structure	465:473	In this study we characterized the structure and composition of polysaccharides from Phellinus linteus and Phellinus igniarius by HPLC, GC-MS and NMR (1-H, 13-C, COSY, NOESY and TOCSY).
26190688	9	24	theme	IL-10	1265:1269	arg1	response					1271:1278	a high IL-10 response	1258:1278	a high IL-10 response	1258:1278	Furthermore, these polysaccharides stimulated a high IL-10 response and induced strong suppression of transcription of IL-6.
26190688	8	25	theme	immuno-suppressive	1183:1200	arg1	activity					1202:1209	an immuno-suppressive activity	1180:1209	an immuno-suppressive activity	1180:1209	In vitro assays by RT-PCR and ELISA showed that (1 → 3; 1 → 6)-β-D-polysaccharides from P. linteus and P. igniarius decreased TNF-α in RAW 264.7 cells, suggesting an immuno-suppressive activity.
26190688	7	26	dep	-β-D-Glcp-	991:1000	arg1	chain					1010:1014	1→ side chain	1002:1014	→6)-β-D-Glcp-(1→ side chain	988:1014	The two-dimensional COSY, NOESY and TOCSY confirmed that these polysaccharides have a main chain of →3)-β-D-Glcp-(1→ with →6)-β-D-Glcp-(1→ side chain.
26190688	10	27	theme	applications	1431:1442	arg1	disturbance					1485:1495	the disturbance	1481:1495	the disturbance	1481:1495	The results suggest that polysaccharides from P. linteus and P. igniarius could possibly find applications in restoring the IL-6/IL-10 balance, the disturbance of which is thought to be related to chronic inflammatory disease, obesity, diabetes type 2, and to mania and depression.
26190688	10	27	theme	applications	1431:1442	arg1	related					1523:1529	related	1523:1529	related	1523:1529	The results suggest that polysaccharides from P. linteus and P. igniarius could possibly find applications in restoring the IL-6/IL-10 balance, the disturbance of which is thought to be related to chronic inflammatory disease, obesity, diabetes type 2, and to mania and depression.
26190688	3	28	theme	fruiting	375:382	arg1	bodies					384:389	their fruiting bodies	369:389	their fruiting bodies	369:389	Polysaccharides from their fruiting bodies show strong immunomodulatory activity.
26190688	0	29	from	effects	49:55	arg1	linteus					91:97	Phellinus linteus	81:97	Phellinus linteus	81:97	Structural characterization and immunomodulatory effects of polysaccharides from Phellinus linteus and Phellinus igniarius on the IL-6/IL-10 cytokine balance of the mouse macrophage cell lines (RAW 264.7).
26190688	0	29	from	effects	49:55	arg1	igniarius					113:121	Phellinus igniarius	103:121	Phellinus igniarius	103:121	Structural characterization and immunomodulatory effects of polysaccharides from Phellinus linteus and Phellinus igniarius on the IL-6/IL-10 cytokine balance of the mouse macrophage cell lines (RAW 264.7).
26190688	0	29	from	effects	49:55	arg1	balance					150:156	the IL-6/IL-10 cytokine balance	126:156	the IL-6/IL-10 cytokine balance of the mouse macrophage cell lines (RAW 264.7)	126:203	Structural characterization and immunomodulatory effects of polysaccharides from Phellinus linteus and Phellinus igniarius on the IL-6/IL-10 cytokine balance of the mouse macrophage cell lines (RAW 264.7).
26190688	4	30	from	composition	479:489	arg1	linteus					525:531	Phellinus linteus	515:531	Phellinus linteus	515:531	In this study we characterized the structure and composition of polysaccharides from Phellinus linteus and Phellinus igniarius by HPLC, GC-MS and NMR (1-H, 13-C, COSY, NOESY and TOCSY).
26190688	4	30	from	composition	479:489	arg1	igniarius					547:555	Phellinus igniarius	537:555	Phellinus igniarius	537:555	In this study we characterized the structure and composition of polysaccharides from Phellinus linteus and Phellinus igniarius by HPLC, GC-MS and NMR (1-H, 13-C, COSY, NOESY and TOCSY).
26190688	7	31	theme	two-dimensional	870:884	arg1	COSY					886:889	The two-dimensional COSY	866:889	The two-dimensional COSY	866:889	The two-dimensional COSY, NOESY and TOCSY confirmed that these polysaccharides have a main chain of →3)-β-D-Glcp-(1→ with →6)-β-D-Glcp-(1→ side chain.
26190688	9	32	theme	IL-6	1331:1334	arg1	transcription					1314:1326	transcription	1314:1326	transcription of IL-6	1314:1334	Furthermore, these polysaccharides stimulated a high IL-10 response and induced strong suppression of transcription of IL-6.
26190688	0	33	theme	Structural	0:9	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and immunomodulatory effects of polysaccharides from Phellinus linteus and Phellinus igniarius on the IL-6/IL-10 cytokine balance of the mouse macrophage cell lines (RAW 264.7).
26190688	8	34	dep	In	1017:1018	arg1	vitro					1020:1024	vitro	1020:1024	vitro	1020:1024	In vitro assays by RT-PCR and ELISA showed that (1 → 3; 1 → 6)-β-D-polysaccharides from P. linteus and P. igniarius decreased TNF-α in RAW 264.7 cells, suggesting an immuno-suppressive activity.
26190688	8	35	dep	-β-D-polysaccharides	1079:1098	arg1	→					1068:1068	1 → 3	1066:1070	1 → 3; 1 → 6	1066:1077	In vitro assays by RT-PCR and ELISA showed that (1 → 3; 1 → 6)-β-D-polysaccharides from P. linteus and P. igniarius decreased TNF-α in RAW 264.7 cells, suggesting an immuno-suppressive activity.
26190688	8	36	theme	P.	1105:1106	arg1	linteus					1108:1114	P. linteus	1105:1114	P. linteus	1105:1114	In vitro assays by RT-PCR and ELISA showed that (1 → 3; 1 → 6)-β-D-polysaccharides from P. linteus and P. igniarius decreased TNF-α in RAW 264.7 cells, suggesting an immuno-suppressive activity.
26190688	9	37	theme	transcription	1314:1326	arg1	suppression					1299:1309	strong suppression	1292:1309	strong suppression of transcription of IL-6	1292:1334	Furthermore, these polysaccharides stimulated a high IL-10 response and induced strong suppression of transcription of IL-6.
26190688	0	38	theme	immunomodulatory	32:47	arg1	effects					49:55	immunomodulatory effects	32:55	immunomodulatory effects of polysaccharides from Phellinus linteus and Phellinus igniarius on the IL-6/IL-10 cytokine balance of the mouse macrophage cell lines (RAW 264.7)	32:203	Structural characterization and immunomodulatory effects of polysaccharides from Phellinus linteus and Phellinus igniarius on the IL-6/IL-10 cytokine balance of the mouse macrophage cell lines (RAW 264.7).
26190688	4	39	from	structure	465:473	arg1	linteus					525:531	Phellinus linteus	515:531	Phellinus linteus	515:531	In this study we characterized the structure and composition of polysaccharides from Phellinus linteus and Phellinus igniarius by HPLC, GC-MS and NMR (1-H, 13-C, COSY, NOESY and TOCSY).
26190688	4	39	from	structure	465:473	arg1	igniarius					547:555	Phellinus igniarius	537:555	Phellinus igniarius	537:555	In this study we characterized the structure and composition of polysaccharides from Phellinus linteus and Phellinus igniarius by HPLC, GC-MS and NMR (1-H, 13-C, COSY, NOESY and TOCSY).
26190688	9	40	theme	strong	1292:1297	arg1	suppression					1299:1309	strong suppression	1292:1309	strong suppression of transcription of IL-6	1292:1334	Furthermore, these polysaccharides stimulated a high IL-10 response and induced strong suppression of transcription of IL-6.
26190688	7	41	theme	main	952:955	arg1	chain					957:961	a main chain	950:961	a main chain of →3)-β-D-Glcp-(1→ with →6)-β-D-Glcp-(1→ side chain	950:1014	The two-dimensional COSY, NOESY and TOCSY confirmed that these polysaccharides have a main chain of →3)-β-D-Glcp-(1→ with →6)-β-D-Glcp-(1→ side chain.
26190688	5	42	contain	contained	676:684	arg1	polysaccharides					620:634	The polysaccharides	616:634	The polysaccharides from P. linteus and P. igniarius	616:667	The polysaccharides from P. linteus and P. igniarius mainly contained glucose with minor proportions of mannose, galactose, xylose, arabinose and rhamnose.
26190688	5	42	contain	contained	676:684	arg2	glucose					686:692	glucose	686:692	glucose	686:692	The polysaccharides from P. linteus and P. igniarius mainly contained glucose with minor proportions of mannose, galactose, xylose, arabinose and rhamnose.
26190688	0	43	theme	cytokine	141:148	arg1	balance					150:156	the IL-6/IL-10 cytokine balance	126:156	the IL-6/IL-10 cytokine balance of the mouse macrophage cell lines (RAW 264.7)	126:203	Structural characterization and immunomodulatory effects of polysaccharides from Phellinus linteus and Phellinus igniarius on the IL-6/IL-10 cytokine balance of the mouse macrophage cell lines (RAW 264.7).
26190688	10	44	from	igniarius	1401:1409	arg1	polysaccharides					1362:1376	polysaccharides	1362:1376	polysaccharides from P. linteus and P. igniarius	1362:1409	The results suggest that polysaccharides from P. linteus and P. igniarius could possibly find applications in restoring the IL-6/IL-10 balance, the disturbance of which is thought to be related to chronic inflammatory disease, obesity, diabetes type 2, and to mania and depression.
26190688	10	45	theme	diabetes	1573:1580	arg1	type					1582:1585	diabetes type 2	1573:1587	diabetes type 2	1573:1587	The results suggest that polysaccharides from P. linteus and P. igniarius could possibly find applications in restoring the IL-6/IL-10 balance, the disturbance of which is thought to be related to chronic inflammatory disease, obesity, diabetes type 2, and to mania and depression.
26190688	10	45	theme	diabetes	1573:1580	arg1	disease					1555:1561	chronic inflammatory disease	1534:1561	chronic inflammatory disease	1534:1561	The results suggest that polysaccharides from P. linteus and P. igniarius could possibly find applications in restoring the IL-6/IL-10 balance, the disturbance of which is thought to be related to chronic inflammatory disease, obesity, diabetes type 2, and to mania and depression.
26190688	5	46	theme	rhamnose	762:769	arg1	proportions					705:715	minor proportions	699:715	minor proportions of mannose, galactose, xylose, arabinose and rhamnose	699:769	The polysaccharides from P. linteus and P. igniarius mainly contained glucose with minor proportions of mannose, galactose, xylose, arabinose and rhamnose.
26190688	1	47	theme	Phellinus	206:214	arg1	linteus					216:222	Phellinus linteus	206:222	Phellinus linteus	206:222	Phellinus linteus and igniarius (L.) Quel.
26190688	3	48	theme	immunomodulatory	403:418	arg1	activity					420:427	strong immunomodulatory activity	396:427	strong immunomodulatory activity	396:427	Polysaccharides from their fruiting bodies show strong immunomodulatory activity.
26190688	7	49	theme	-β-D-Glcp-	969:978	arg1	chain					957:961	a main chain	950:961	a main chain of →3)-β-D-Glcp-(1→ with →6)-β-D-Glcp-(1→ side chain	950:1014	The two-dimensional COSY, NOESY and TOCSY confirmed that these polysaccharides have a main chain of →3)-β-D-Glcp-(1→ with →6)-β-D-Glcp-(1→ side chain.
26190688	5	50	theme	arabinose	748:756	arg1	proportions					705:715	minor proportions	699:715	minor proportions of mannose, galactose, xylose, arabinose and rhamnose	699:769	The polysaccharides from P. linteus and P. igniarius mainly contained glucose with minor proportions of mannose, galactose, xylose, arabinose and rhamnose.
26190688	0	51	from	linteus	91:97	arg1	polysaccharides					60:74	polysaccharides	60:74	polysaccharides from Phellinus linteus and Phellinus igniarius	60:121	Structural characterization and immunomodulatory effects of polysaccharides from Phellinus linteus and Phellinus igniarius on the IL-6/IL-10 cytokine balance of the mouse macrophage cell lines (RAW 264.7).
26190688	0	51	from	linteus	91:97	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and immunomodulatory effects of polysaccharides from Phellinus linteus and Phellinus igniarius on the IL-6/IL-10 cytokine balance of the mouse macrophage cell lines (RAW 264.7).
26190688	0	51	from	linteus	91:97	arg1	effects					49:55	immunomodulatory effects	32:55	immunomodulatory effects of polysaccharides from Phellinus linteus and Phellinus igniarius on the IL-6/IL-10 cytokine balance of the mouse macrophage cell lines (RAW 264.7)	32:203	Structural characterization and immunomodulatory effects of polysaccharides from Phellinus linteus and Phellinus igniarius on the IL-6/IL-10 cytokine balance of the mouse macrophage cell lines (RAW 264.7).
26190688	10	52	theme	IL-6/IL-10	1461:1470	arg1	balance					1472:1478	the IL-6/IL-10 balance	1457:1478	the IL-6/IL-10 balance	1457:1478	The results suggest that polysaccharides from P. linteus and P. igniarius could possibly find applications in restoring the IL-6/IL-10 balance, the disturbance of which is thought to be related to chronic inflammatory disease, obesity, diabetes type 2, and to mania and depression.
26190688	5	53	theme	mannose	720:726	arg1	proportions					705:715	minor proportions	699:715	minor proportions of mannose, galactose, xylose, arabinose and rhamnose	699:769	The polysaccharides from P. linteus and P. igniarius mainly contained glucose with minor proportions of mannose, galactose, xylose, arabinose and rhamnose.
26190688	4	54	from	igniarius	547:555	arg1	polysaccharides					494:508	polysaccharides	494:508	polysaccharides from Phellinus linteus and Phellinus igniarius	494:555	In this study we characterized the structure and composition of polysaccharides from Phellinus linteus and Phellinus igniarius by HPLC, GC-MS and NMR (1-H, 13-C, COSY, NOESY and TOCSY).
26190688	4	54	from	igniarius	547:555	arg1	composition					479:489	composition	479:489	composition	479:489	In this study we characterized the structure and composition of polysaccharides from Phellinus linteus and Phellinus igniarius by HPLC, GC-MS and NMR (1-H, 13-C, COSY, NOESY and TOCSY).
26190688	4	54	from	igniarius	547:555	arg1	structure					465:473	structure	465:473	structure	465:473	In this study we characterized the structure and composition of polysaccharides from Phellinus linteus and Phellinus igniarius by HPLC, GC-MS and NMR (1-H, 13-C, COSY, NOESY and TOCSY).
26190688	4	55	theme	Phellinus	537:545	arg1	igniarius					547:555	Phellinus igniarius	537:555	Phellinus igniarius	537:555	In this study we characterized the structure and composition of polysaccharides from Phellinus linteus and Phellinus igniarius by HPLC, GC-MS and NMR (1-H, 13-C, COSY, NOESY and TOCSY).
26190688	0	56	theme	macrophage	171:180	arg1	RAW					194:196	RAW 264.7	194:202	RAW 264.7	194:202	Structural characterization and immunomodulatory effects of polysaccharides from Phellinus linteus and Phellinus igniarius on the IL-6/IL-10 cytokine balance of the mouse macrophage cell lines (RAW 264.7).
26190688	0	56	theme	macrophage	171:180	arg1	lines					187:191	the mouse macrophage cell lines	161:191	the mouse macrophage cell lines (RAW 264.7)	161:203	Structural characterization and immunomodulatory effects of polysaccharides from Phellinus linteus and Phellinus igniarius on the IL-6/IL-10 cytokine balance of the mouse macrophage cell lines (RAW 264.7).
26190688	5	57	theme	galactose	729:737	arg1	proportions					705:715	minor proportions	699:715	minor proportions of mannose, galactose, xylose, arabinose and rhamnose	699:769	The polysaccharides from P. linteus and P. igniarius mainly contained glucose with minor proportions of mannose, galactose, xylose, arabinose and rhamnose.
26190688	4	58	dep	GC-MS	566:570	arg1	COSY					592:595	COSY	592:595	COSY	592:595	In this study we characterized the structure and composition of polysaccharides from Phellinus linteus and Phellinus igniarius by HPLC, GC-MS and NMR (1-H, 13-C, COSY, NOESY and TOCSY).
26190688	4	58	dep	GC-MS	566:570	arg1	13-C					586:589	13-C	586:589	13-C	586:589	In this study we characterized the structure and composition of polysaccharides from Phellinus linteus and Phellinus igniarius by HPLC, GC-MS and NMR (1-H, 13-C, COSY, NOESY and TOCSY).
26190688	4	58	dep	GC-MS	566:570	arg1	NOESY					598:602	NOESY	598:602	NOESY	598:602	In this study we characterized the structure and composition of polysaccharides from Phellinus linteus and Phellinus igniarius by HPLC, GC-MS and NMR (1-H, 13-C, COSY, NOESY and TOCSY).
26190688	4	58	dep	GC-MS	566:570	arg1	1-H					581:583	1-H	581:583	1-H	581:583	In this study we characterized the structure and composition of polysaccharides from Phellinus linteus and Phellinus igniarius by HPLC, GC-MS and NMR (1-H, 13-C, COSY, NOESY and TOCSY).
26190688	4	58	dep	GC-MS	566:570	arg1	TOCSY					608:612	TOCSY	608:612	TOCSY	608:612	In this study we characterized the structure and composition of polysaccharides from Phellinus linteus and Phellinus igniarius by HPLC, GC-MS and NMR (1-H, 13-C, COSY, NOESY and TOCSY).
26190688	0	59	theme	polysaccharides	60:74	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and immunomodulatory effects of polysaccharides from Phellinus linteus and Phellinus igniarius on the IL-6/IL-10 cytokine balance of the mouse macrophage cell lines (RAW 264.7).
26190688	0	59	theme	polysaccharides	60:74	arg1	effects					49:55	immunomodulatory effects	32:55	immunomodulatory effects of polysaccharides from Phellinus linteus and Phellinus igniarius on the IL-6/IL-10 cytokine balance of the mouse macrophage cell lines (RAW 264.7)	32:203	Structural characterization and immunomodulatory effects of polysaccharides from Phellinus linteus and Phellinus igniarius on the IL-6/IL-10 cytokine balance of the mouse macrophage cell lines (RAW 264.7).
26190688	0	60	theme	mouse	165:169	arg1	RAW					194:196	RAW 264.7	194:202	RAW 264.7	194:202	Structural characterization and immunomodulatory effects of polysaccharides from Phellinus linteus and Phellinus igniarius on the IL-6/IL-10 cytokine balance of the mouse macrophage cell lines (RAW 264.7).
26190688	0	60	theme	mouse	165:169	arg1	lines					187:191	the mouse macrophage cell lines	161:191	the mouse macrophage cell lines (RAW 264.7)	161:203	Structural characterization and immunomodulatory effects of polysaccharides from Phellinus linteus and Phellinus igniarius on the IL-6/IL-10 cytokine balance of the mouse macrophage cell lines (RAW 264.7).
26190688	0	61	theme	lines	187:191	arg1	balance					150:156	the IL-6/IL-10 cytokine balance	126:156	the IL-6/IL-10 cytokine balance of the mouse macrophage cell lines (RAW 264.7)	126:203	Structural characterization and immunomodulatory effects of polysaccharides from Phellinus linteus and Phellinus igniarius on the IL-6/IL-10 cytokine balance of the mouse macrophage cell lines (RAW 264.7).
26190688	6	62	theme	glycosidic	809:818	arg1	linkages					820:827	the glycosidic linkages	805:827	the glycosidic linkages	805:827	Methylation analyses showed that the glycosidic linkages were mostly 1 → 3, 1 → 6 or 1 → 3,6.
26190688	6	62	theme	glycosidic	809:818	arg1	1					841:841	1	841:841	1	841:841	Methylation analyses showed that the glycosidic linkages were mostly 1 → 3, 1 → 6 or 1 → 3,6.
26190688	0	63	theme	cell	182:185	arg1	RAW					194:196	RAW 264.7	194:202	RAW 264.7	194:202	Structural characterization and immunomodulatory effects of polysaccharides from Phellinus linteus and Phellinus igniarius on the IL-6/IL-10 cytokine balance of the mouse macrophage cell lines (RAW 264.7).
26190688	0	63	theme	cell	182:185	arg1	lines					187:191	the mouse macrophage cell lines	161:191	the mouse macrophage cell lines (RAW 264.7)	161:203	Structural characterization and immunomodulatory effects of polysaccharides from Phellinus linteus and Phellinus igniarius on the IL-6/IL-10 cytokine balance of the mouse macrophage cell lines (RAW 264.7).
26190688	10	64	theme	inflammatory	1542:1553	arg1	obesity					1564:1570	obesity	1564:1570	obesity	1564:1570	The results suggest that polysaccharides from P. linteus and P. igniarius could possibly find applications in restoring the IL-6/IL-10 balance, the disturbance of which is thought to be related to chronic inflammatory disease, obesity, diabetes type 2, and to mania and depression.
26190688	10	64	theme	inflammatory	1542:1553	arg1	type					1582:1585	diabetes type 2	1573:1587	diabetes type 2	1573:1587	The results suggest that polysaccharides from P. linteus and P. igniarius could possibly find applications in restoring the IL-6/IL-10 balance, the disturbance of which is thought to be related to chronic inflammatory disease, obesity, diabetes type 2, and to mania and depression.
26190688	10	64	theme	inflammatory	1542:1553	arg1	disease					1555:1561	chronic inflammatory disease	1534:1561	chronic inflammatory disease	1534:1561	The results suggest that polysaccharides from P. linteus and P. igniarius could possibly find applications in restoring the IL-6/IL-10 balance, the disturbance of which is thought to be related to chronic inflammatory disease, obesity, diabetes type 2, and to mania and depression.
26190688	5	65	from	igniarius	659:667	arg1	polysaccharides					620:634	The polysaccharides	616:634	The polysaccharides from P. linteus and P. igniarius	616:667	The polysaccharides from P. linteus and P. igniarius mainly contained glucose with minor proportions of mannose, galactose, xylose, arabinose and rhamnose.
26190688	7	66	dep	-β-D-Glcp-	969:978	arg1	→3					966:967	→3	966:967	→3	966:967	The two-dimensional COSY, NOESY and TOCSY confirmed that these polysaccharides have a main chain of →3)-β-D-Glcp-(1→ with →6)-β-D-Glcp-(1→ side chain.
26190688	0	67	from	igniarius	113:121	arg1	polysaccharides					60:74	polysaccharides	60:74	polysaccharides from Phellinus linteus and Phellinus igniarius	60:121	Structural characterization and immunomodulatory effects of polysaccharides from Phellinus linteus and Phellinus igniarius on the IL-6/IL-10 cytokine balance of the mouse macrophage cell lines (RAW 264.7).
26190688	0	67	from	igniarius	113:121	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and immunomodulatory effects of polysaccharides from Phellinus linteus and Phellinus igniarius on the IL-6/IL-10 cytokine balance of the mouse macrophage cell lines (RAW 264.7).
26190688	0	67	from	igniarius	113:121	arg1	effects					49:55	immunomodulatory effects	32:55	immunomodulatory effects of polysaccharides from Phellinus linteus and Phellinus igniarius on the IL-6/IL-10 cytokine balance of the mouse macrophage cell lines (RAW 264.7)	32:203	Structural characterization and immunomodulatory effects of polysaccharides from Phellinus linteus and Phellinus igniarius on the IL-6/IL-10 cytokine balance of the mouse macrophage cell lines (RAW 264.7).
26190688	10	68	theme	P.	1383:1384	arg1	linteus					1386:1392	P. linteus	1383:1392	P. linteus	1383:1392	The results suggest that polysaccharides from P. linteus and P. igniarius could possibly find applications in restoring the IL-6/IL-10 balance, the disturbance of which is thought to be related to chronic inflammatory disease, obesity, diabetes type 2, and to mania and depression.
26190688	5	69	from	linteus	644:650	arg1	polysaccharides					620:634	The polysaccharides	616:634	The polysaccharides from P. linteus and P. igniarius	616:667	The polysaccharides from P. linteus and P. igniarius mainly contained glucose with minor proportions of mannose, galactose, xylose, arabinose and rhamnose.
26190688	0	70	theme	Phellinus	81:89	arg1	linteus					91:97	Phellinus linteus	81:97	Phellinus linteus	81:97	Structural characterization and immunomodulatory effects of polysaccharides from Phellinus linteus and Phellinus igniarius on the IL-6/IL-10 cytokine balance of the mouse macrophage cell lines (RAW 264.7).
26190688	4	71	theme	Phellinus	515:523	arg1	linteus					525:531	Phellinus linteus	515:531	Phellinus linteus	515:531	In this study we characterized the structure and composition of polysaccharides from Phellinus linteus and Phellinus igniarius by HPLC, GC-MS and NMR (1-H, 13-C, COSY, NOESY and TOCSY).
26190688	8	72	from	linteus	1108:1114	arg1	-β-D-polysaccharides					1079:1098	(1 → 3; 1 → 6)-β-D-polysaccharides	1065:1098	(1 → 3; 1 → 6)-β-D-polysaccharides from P. linteus	1065:1114	In vitro assays by RT-PCR and ELISA showed that (1 → 3; 1 → 6)-β-D-polysaccharides from P. linteus and P. igniarius decreased TNF-α in RAW 264.7 cells, suggesting an immuno-suppressive activity.
26190688	8	73	theme	RAW	1152:1154	arg1	cells					1162:1166	RAW 264.7 cells	1152:1166	RAW 264.7 cells	1152:1166	In vitro assays by RT-PCR and ELISA showed that (1 → 3; 1 → 6)-β-D-polysaccharides from P. linteus and P. igniarius decreased TNF-α in RAW 264.7 cells, suggesting an immuno-suppressive activity.
26190688	9	74	theme	high	1260:1263	arg1	response					1271:1278	a high IL-10 response	1258:1278	a high IL-10 response	1258:1278	Furthermore, these polysaccharides stimulated a high IL-10 response and induced strong suppression of transcription of IL-6.
28363654	0	0	theme	composition	85:95	arg1	Optimization					63:74	Optimization	63:74	Production of exopolysaccharides by selected Bacillus strains: Optimization of media composition to maximize the yield and structural characterization.	0:150	Production of exopolysaccharides by selected Bacillus strains: Optimization of media composition to maximize the yield and structural characterization.
28363654	5	1	theme	response	1260:1267	arg1	methodology					1277:1287	response surface methodology	1260:1287	response surface methodology analysis	1260:1296	The effect of the concentrations of yeast extract, sodium succinate and sucrose on the EPS production by B. licheniformis 14580 were studied using response surface methodology analysis.
28363654	1	2	dep	tryptone	434:441	arg1	iii					429:431	iii	429:431	iii	429:431	Selected Bacillus strains were investigated for the production of exopolysaccharides (EPS) by fermentation in three different culture media: (i) mineral base-medium with added yeast extract (M1), (ii) succinate-containing mineral base-medium with added yeast extract (M2) and (iii) tryptone and yeast extract-containing base-medium (M3).
28363654	3	3	with	profile	866:872	arg1	galactose					879:887	galactose	879:887	galactose	879:887	The most significant yield (48.57g/L) was obtained with Bacillus licheniformis 14580 in M2; the EPS produced was 5-30kDa in size and characterized by an exceptionally heterogeneous monosaccharide profile with galactose, fructose and glucose as the predominating monomers.
28363654	3	3	with	profile	866:872	arg1	glucose					903:909	glucose	903:909	glucose	903:909	The most significant yield (48.57g/L) was obtained with Bacillus licheniformis 14580 in M2; the EPS produced was 5-30kDa in size and characterized by an exceptionally heterogeneous monosaccharide profile with galactose, fructose and glucose as the predominating monomers.
28363654	3	3	with	profile	866:872	arg1	fructose					890:897	fructose	890:897	fructose	890:897	The most significant yield (48.57g/L) was obtained with Bacillus licheniformis 14580 in M2; the EPS produced was 5-30kDa in size and characterized by an exceptionally heterogeneous monosaccharide profile with galactose, fructose and glucose as the predominating monomers.
28363654	0	4	theme	media	79:83	arg1	composition					85:95	media composition	79:95	media composition to maximize the yield and structural characterization	79:149	Production of exopolysaccharides by selected Bacillus strains: Optimization of media composition to maximize the yield and structural characterization.
28363654	5	5	theme	extract	1155:1161	arg1	concentrations					1131:1144	the concentrations	1127:1144	the concentrations of yeast extract, sodium succinate and sucrose	1127:1191	The effect of the concentrations of yeast extract, sodium succinate and sucrose on the EPS production by B. licheniformis 14580 were studied using response surface methodology analysis.
28363654	1	6	theme	different	268:276	arg1	media					286:290	three different culture media	262:290	three different culture media	262:290	Selected Bacillus strains were investigated for the production of exopolysaccharides (EPS) by fermentation in three different culture media: (i) mineral base-medium with added yeast extract (M1), (ii) succinate-containing mineral base-medium with added yeast extract (M2) and (iii) tryptone and yeast extract-containing base-medium (M3).
28363654	4	7	theme	obtained	1040:1047	arg1	EPS					1064:1066	the obtained heteropolymers EPS	1036:1066	the obtained heteropolymers EPS	1036:1066	The use of M1 medium for the growth of B. licheniformis 14580 resulted in low yield; however, the obtained heteropolymers EPS were characterized by a higher MW 30-100kDa.
28363654	5	8	theme	yeast	1149:1153	arg1	extract					1155:1161	yeast extract	1149:1161	yeast extract	1149:1161	The effect of the concentrations of yeast extract, sodium succinate and sucrose on the EPS production by B. licheniformis 14580 were studied using response surface methodology analysis.
28363654	6	9	theme	decreased	1403:1411	arg1	concentrations					1413:1426	decreased concentrations	1403:1426	decreased concentrations of yeast extract in the mineral media	1403:1464	Greater EPS yields were achieved with increased concentrations of sodium succinate and sucrose and with decreased concentrations of yeast extract in the mineral media.
28363654	1	10	with	extract	334:340	arg1	extract					411:417	added yeast extract	399:417	added yeast extract (M2)	399:422	Selected Bacillus strains were investigated for the production of exopolysaccharides (EPS) by fermentation in three different culture media: (i) mineral base-medium with added yeast extract (M1), (ii) succinate-containing mineral base-medium with added yeast extract (M2) and (iii) tryptone and yeast extract-containing base-medium (M3).
28363654	1	10	with	extract	334:340	arg1	M2					420:421	M2	420:421	M2	420:421	Selected Bacillus strains were investigated for the production of exopolysaccharides (EPS) by fermentation in three different culture media: (i) mineral base-medium with added yeast extract (M1), (ii) succinate-containing mineral base-medium with added yeast extract (M2) and (iii) tryptone and yeast extract-containing base-medium (M3).
28363654	1	10	with	extract	334:340	arg1	tryptone					434:441	(iii) tryptone	428:441	(iii) tryptone	428:441	Selected Bacillus strains were investigated for the production of exopolysaccharides (EPS) by fermentation in three different culture media: (i) mineral base-medium with added yeast extract (M1), (ii) succinate-containing mineral base-medium with added yeast extract (M2) and (iii) tryptone and yeast extract-containing base-medium (M3).
28363654	1	11	theme	culture	278:284	arg1	media					286:290	three different culture media	262:290	three different culture media	262:290	Selected Bacillus strains were investigated for the production of exopolysaccharides (EPS) by fermentation in three different culture media: (i) mineral base-medium with added yeast extract (M1), (ii) succinate-containing mineral base-medium with added yeast extract (M2) and (iii) tryptone and yeast extract-containing base-medium (M3).
28363654	5	12	theme	methodology	1277:1287	arg1	analysis					1289:1296	response surface methodology analysis	1260:1296	response surface methodology analysis	1260:1296	The effect of the concentrations of yeast extract, sodium succinate and sucrose on the EPS production by B. licheniformis 14580 were studied using response surface methodology analysis.
28363654	1	13	with	base-medium	305:315	arg1	M3					485:486	M3	485:486	M3	485:486	Selected Bacillus strains were investigated for the production of exopolysaccharides (EPS) by fermentation in three different culture media: (i) mineral base-medium with added yeast extract (M1), (ii) succinate-containing mineral base-medium with added yeast extract (M2) and (iii) tryptone and yeast extract-containing base-medium (M3).
28363654	1	13	with	base-medium	305:315	arg1	base-medium					382:392	(ii) succinate-containing mineral base-medium	348:392	(ii) succinate-containing mineral base-medium with added yeast extract (M2) and (iii) tryptone	348:441	Selected Bacillus strains were investigated for the production of exopolysaccharides (EPS) by fermentation in three different culture media: (i) mineral base-medium with added yeast extract (M1), (ii) succinate-containing mineral base-medium with added yeast extract (M2) and (iii) tryptone and yeast extract-containing base-medium (M3).
28363654	1	13	with	base-medium	305:315	arg1	M1					343:344	M1	343:344	M1	343:344	Selected Bacillus strains were investigated for the production of exopolysaccharides (EPS) by fermentation in three different culture media: (i) mineral base-medium with added yeast extract (M1), (ii) succinate-containing mineral base-medium with added yeast extract (M2) and (iii) tryptone and yeast extract-containing base-medium (M3).
28363654	1	13	with	base-medium	305:315	arg1	extract					334:340	added yeast extract	322:340	added yeast extract (M1)	322:345	Selected Bacillus strains were investigated for the production of exopolysaccharides (EPS) by fermentation in three different culture media: (i) mineral base-medium with added yeast extract (M1), (ii) succinate-containing mineral base-medium with added yeast extract (M2) and (iii) tryptone and yeast extract-containing base-medium (M3).
28363654	1	13	with	base-medium	305:315	arg1	base-medium					472:482	yeast extract-containing base-medium	447:482	yeast extract-containing base-medium (M3)	447:487	Selected Bacillus strains were investigated for the production of exopolysaccharides (EPS) by fermentation in three different culture media: (i) mineral base-medium with added yeast extract (M1), (ii) succinate-containing mineral base-medium with added yeast extract (M2) and (iii) tryptone and yeast extract-containing base-medium (M3).
28363654	3	14	from	5-30kDa	783:789	arg1	size					794:797	size	794:797	size	794:797	The most significant yield (48.57g/L) was obtained with Bacillus licheniformis 14580 in M2; the EPS produced was 5-30kDa in size and characterized by an exceptionally heterogeneous monosaccharide profile with galactose, fructose and glucose as the predominating monomers.
28363654	5	15	theme	B.	1218:1219	arg1	14580					1235:1239	B. licheniformis 14580	1218:1239	B. licheniformis 14580	1218:1239	The effect of the concentrations of yeast extract, sodium succinate and sucrose on the EPS production by B. licheniformis 14580 were studied using response surface methodology analysis.
28363654	6	16	theme	extract	1437:1443	arg1	concentrations					1413:1426	decreased concentrations	1403:1426	decreased concentrations of yeast extract in the mineral media	1403:1464	Greater EPS yields were achieved with increased concentrations of sodium succinate and sucrose and with decreased concentrations of yeast extract in the mineral media.
28363654	2	17	dep	amyloliquefaciens	543:559	arg1	23350					561:565	23350	561:565	23350	561:565	Modest EPS yield of 6.7g/L was recorded for Bacillus amyloliquefaciens 23350 grown on M1, where the EPS produced was characterized by a low MW (<5kDa) and of being mainly glucans.
28363654	2	18	theme	low	626:628	arg1	<5kDa					634:638	<5kDa	634:638	<5kDa	634:638	Modest EPS yield of 6.7g/L was recorded for Bacillus amyloliquefaciens 23350 grown on M1, where the EPS produced was characterized by a low MW (<5kDa) and of being mainly glucans.
28363654	2	18	theme	low	626:628	arg1	MW					630:631	a low MW	624:631	a low MW (<5kDa)	624:639	Modest EPS yield of 6.7g/L was recorded for Bacillus amyloliquefaciens 23350 grown on M1, where the EPS produced was characterized by a low MW (<5kDa) and of being mainly glucans.
28363654	6	19	theme	increased	1337:1345	arg1	concentrations					1347:1360	increased concentrations	1337:1360	increased concentrations of sodium succinate and sucrose	1337:1392	Greater EPS yields were achieved with increased concentrations of sodium succinate and sucrose and with decreased concentrations of yeast extract in the mineral media.
28363654	5	20	theme	licheniformis	1221:1233	arg1	14580					1235:1239	B. licheniformis 14580	1218:1239	B. licheniformis 14580	1218:1239	The effect of the concentrations of yeast extract, sodium succinate and sucrose on the EPS production by B. licheniformis 14580 were studied using response surface methodology analysis.
28363654	4	21	theme	medium	956:961	arg1	use					946:948	The use	942:948	The use of M1 medium for the growth of B. licheniformis 14580	942:1002	The use of M1 medium for the growth of B. licheniformis 14580 resulted in low yield; however, the obtained heteropolymers EPS were characterized by a higher MW 30-100kDa.
28363654	1	22	dep	base-medium	305:315	arg1	i					294:294	i	294:294	i	294:294	Selected Bacillus strains were investigated for the production of exopolysaccharides (EPS) by fermentation in three different culture media: (i) mineral base-medium with added yeast extract (M1), (ii) succinate-containing mineral base-medium with added yeast extract (M2) and (iii) tryptone and yeast extract-containing base-medium (M3).
28363654	6	23	theme	succinate	1372:1380	arg1	concentrations					1347:1360	increased concentrations	1337:1360	increased concentrations of sodium succinate and sucrose	1337:1392	Greater EPS yields were achieved with increased concentrations of sodium succinate and sucrose and with decreased concentrations of yeast extract in the mineral media.
28363654	4	24	theme	MW	1099:1100	arg1	30-100kDa					1102:1110	a higher MW 30-100kDa	1090:1110	a higher MW 30-100kDa	1090:1110	The use of M1 medium for the growth of B. licheniformis 14580 resulted in low yield; however, the obtained heteropolymers EPS were characterized by a higher MW 30-100kDa.
28363654	6	25	theme	sodium	1365:1370	arg1	succinate					1372:1380	sodium succinate	1365:1380	sodium succinate	1365:1380	Greater EPS yields were achieved with increased concentrations of sodium succinate and sucrose and with decreased concentrations of yeast extract in the mineral media.
28363654	3	26	from	14580	749:753	arg1	M2					758:759	M2	758:759	M2	758:759	The most significant yield (48.57g/L) was obtained with Bacillus licheniformis 14580 in M2; the EPS produced was 5-30kDa in size and characterized by an exceptionally heterogeneous monosaccharide profile with galactose, fructose and glucose as the predominating monomers.
28363654	6	27	theme	yeast	1431:1435	arg1	extract					1437:1443	yeast extract	1431:1443	yeast extract in the mineral media	1431:1464	Greater EPS yields were achieved with increased concentrations of sodium succinate and sucrose and with decreased concentrations of yeast extract in the mineral media.
28363654	6	28	from	concentrations	1413:1426	arg1	media					1460:1464	the mineral media	1448:1464	the mineral media	1448:1464	Greater EPS yields were achieved with increased concentrations of sodium succinate and sucrose and with decreased concentrations of yeast extract in the mineral media.
28363654	3	29	theme	heterogeneous	837:849	arg1	profile					866:872	an exceptionally heterogeneous monosaccharide profile	820:872	an exceptionally heterogeneous monosaccharide profile with galactose, fructose and glucose	820:909	The most significant yield (48.57g/L) was obtained with Bacillus licheniformis 14580 in M2; the EPS produced was 5-30kDa in size and characterized by an exceptionally heterogeneous monosaccharide profile with galactose, fructose and glucose as the predominating monomers.
28363654	4	30	theme	M1	953:954	arg1	medium					956:961	M1 medium	953:961	M1 medium	953:961	The use of M1 medium for the growth of B. licheniformis 14580 resulted in low yield; however, the obtained heteropolymers EPS were characterized by a higher MW 30-100kDa.
28363654	0	31	theme	structural	123:132	arg1	characterization					134:149	structural characterization	123:149	structural characterization	123:149	Production of exopolysaccharides by selected Bacillus strains: Optimization of media composition to maximize the yield and structural characterization.
28363654	3	32	theme	predominating	918:930	arg1	monomers					932:939	the predominating monomers	914:939	the predominating monomers	914:939	The most significant yield (48.57g/L) was obtained with Bacillus licheniformis 14580 in M2; the EPS produced was 5-30kDa in size and characterized by an exceptionally heterogeneous monosaccharide profile with galactose, fructose and glucose as the predominating monomers.
28363654	3	32	theme	predominating	918:930	arg1	EPS					766:768	the EPS	762:768	the EPS produced	762:777	The most significant yield (48.57g/L) was obtained with Bacillus licheniformis 14580 in M2; the EPS produced was 5-30kDa in size and characterized by an exceptionally heterogeneous monosaccharide profile with galactose, fructose and glucose as the predominating monomers.
28363654	1	33	dep	Selected	152:159	arg1	base-medium					305:315	(i) mineral base-medium	293:315	(i) mineral base-medium with added yeast extract (M1), (ii) succinate-containing mineral base-medium with added yeast extract (M2) and (iii) tryptone and yeast extract-containing base-medium (M3)	293:487	Selected Bacillus strains were investigated for the production of exopolysaccharides (EPS) by fermentation in three different culture media: (i) mineral base-medium with added yeast extract (M1), (ii) succinate-containing mineral base-medium with added yeast extract (M2) and (iii) tryptone and yeast extract-containing base-medium (M3).
28363654	1	34	with	base-medium	382:392	arg1	extract					411:417	added yeast extract	399:417	added yeast extract (M2)	399:422	Selected Bacillus strains were investigated for the production of exopolysaccharides (EPS) by fermentation in three different culture media: (i) mineral base-medium with added yeast extract (M1), (ii) succinate-containing mineral base-medium with added yeast extract (M2) and (iii) tryptone and yeast extract-containing base-medium (M3).
28363654	1	34	with	base-medium	382:392	arg1	M2					420:421	M2	420:421	M2	420:421	Selected Bacillus strains were investigated for the production of exopolysaccharides (EPS) by fermentation in three different culture media: (i) mineral base-medium with added yeast extract (M1), (ii) succinate-containing mineral base-medium with added yeast extract (M2) and (iii) tryptone and yeast extract-containing base-medium (M3).
28363654	1	34	with	base-medium	382:392	arg1	tryptone					434:441	(iii) tryptone	428:441	(iii) tryptone	428:441	Selected Bacillus strains were investigated for the production of exopolysaccharides (EPS) by fermentation in three different culture media: (i) mineral base-medium with added yeast extract (M1), (ii) succinate-containing mineral base-medium with added yeast extract (M2) and (iii) tryptone and yeast extract-containing base-medium (M3).
28363654	0	35	theme	exopolysaccharides	14:31	arg1	Production					0:9	Production	0:9	Production of exopolysaccharides by selected Bacillus strains: Optimization of media composition to maximize the yield and structural characterization.	0:150	Production of exopolysaccharides by selected Bacillus strains: Optimization of media composition to maximize the yield and structural characterization.
28363654	4	36	theme	higher	1092:1097	arg1	30-100kDa					1102:1110	a higher MW 30-100kDa	1090:1110	a higher MW 30-100kDa	1090:1110	The use of M1 medium for the growth of B. licheniformis 14580 resulted in low yield; however, the obtained heteropolymers EPS were characterized by a higher MW 30-100kDa.
28363654	5	37	theme	EPS	1200:1202	arg1	production					1204:1213	the EPS production	1196:1213	the EPS production by B. licheniformis 14580	1196:1239	The effect of the concentrations of yeast extract, sodium succinate and sucrose on the EPS production by B. licheniformis 14580 were studied using response surface methodology analysis.
28363654	1	38	theme	exopolysaccharides	218:235	arg1	production					204:213	the production	200:213	the production of exopolysaccharides (EPS) by fermentation in three different culture media	200:290	Selected Bacillus strains were investigated for the production of exopolysaccharides (EPS) by fermentation in three different culture media: (i) mineral base-medium with added yeast extract (M1), (ii) succinate-containing mineral base-medium with added yeast extract (M2) and (iii) tryptone and yeast extract-containing base-medium (M3).
28363654	0	39	theme	Bacillus	45:52	arg1	strains					54:60	selected Bacillus strains	36:60	selected Bacillus strains	36:60	Production of exopolysaccharides by selected Bacillus strains: Optimization of media composition to maximize the yield and structural characterization.
28363654	6	40	theme	mineral	1452:1458	arg1	media					1460:1464	the mineral media	1448:1464	the mineral media	1448:1464	Greater EPS yields were achieved with increased concentrations of sodium succinate and sucrose and with decreased concentrations of yeast extract in the mineral media.
28363654	2	41	theme	EPS	497:499	arg1	yield					501:505	Modest EPS yield	490:505	Modest EPS yield of 6.7g/L	490:515	Modest EPS yield of 6.7g/L was recorded for Bacillus amyloliquefaciens 23350 grown on M1, where the EPS produced was characterized by a low MW (<5kDa) and of being mainly glucans.
28363654	1	42	theme	succinate-containing	353:372	arg1	base-medium					382:392	(ii) succinate-containing mineral base-medium	348:392	(ii) succinate-containing mineral base-medium with added yeast extract (M2) and (iii) tryptone	348:441	Selected Bacillus strains were investigated for the production of exopolysaccharides (EPS) by fermentation in three different culture media: (i) mineral base-medium with added yeast extract (M1), (ii) succinate-containing mineral base-medium with added yeast extract (M2) and (iii) tryptone and yeast extract-containing base-medium (M3).
28363654	4	43	theme	low	1016:1018	arg1	yield					1020:1024	low yield	1016:1024	low yield	1016:1024	The use of M1 medium for the growth of B. licheniformis 14580 resulted in low yield; however, the obtained heteropolymers EPS were characterized by a higher MW 30-100kDa.
28363654	0	44	theme	selected	36:43	arg1	strains					54:60	selected Bacillus strains	36:60	selected Bacillus strains	36:60	Production of exopolysaccharides by selected Bacillus strains: Optimization of media composition to maximize the yield and structural characterization.
28363654	1	45	theme	mineral	297:303	arg1	base-medium					305:315	(i) mineral base-medium	293:315	(i) mineral base-medium with added yeast extract (M1), (ii) succinate-containing mineral base-medium with added yeast extract (M2) and (iii) tryptone and yeast extract-containing base-medium (M3)	293:487	Selected Bacillus strains were investigated for the production of exopolysaccharides (EPS) by fermentation in three different culture media: (i) mineral base-medium with added yeast extract (M1), (ii) succinate-containing mineral base-medium with added yeast extract (M2) and (iii) tryptone and yeast extract-containing base-medium (M3).
28363654	1	46	with	base-medium	472:482	arg1	extract					411:417	added yeast extract	399:417	added yeast extract (M2)	399:422	Selected Bacillus strains were investigated for the production of exopolysaccharides (EPS) by fermentation in three different culture media: (i) mineral base-medium with added yeast extract (M1), (ii) succinate-containing mineral base-medium with added yeast extract (M2) and (iii) tryptone and yeast extract-containing base-medium (M3).
28363654	1	46	with	base-medium	472:482	arg1	M2					420:421	M2	420:421	M2	420:421	Selected Bacillus strains were investigated for the production of exopolysaccharides (EPS) by fermentation in three different culture media: (i) mineral base-medium with added yeast extract (M1), (ii) succinate-containing mineral base-medium with added yeast extract (M2) and (iii) tryptone and yeast extract-containing base-medium (M3).
28363654	1	46	with	base-medium	472:482	arg1	tryptone					434:441	(iii) tryptone	428:441	(iii) tryptone	428:441	Selected Bacillus strains were investigated for the production of exopolysaccharides (EPS) by fermentation in three different culture media: (i) mineral base-medium with added yeast extract (M1), (ii) succinate-containing mineral base-medium with added yeast extract (M2) and (iii) tryptone and yeast extract-containing base-medium (M3).
28363654	1	47	theme	mineral	374:380	arg1	base-medium					382:392	(ii) succinate-containing mineral base-medium	348:392	(ii) succinate-containing mineral base-medium with added yeast extract (M2) and (iii) tryptone	348:441	Selected Bacillus strains were investigated for the production of exopolysaccharides (EPS) by fermentation in three different culture media: (i) mineral base-medium with added yeast extract (M1), (ii) succinate-containing mineral base-medium with added yeast extract (M2) and (iii) tryptone and yeast extract-containing base-medium (M3).
28363654	5	48	theme	surface	1269:1275	arg1	methodology					1277:1287	response surface methodology	1260:1287	response surface methodology analysis	1260:1296	The effect of the concentrations of yeast extract, sodium succinate and sucrose on the EPS production by B. licheniformis 14580 were studied using response surface methodology analysis.
28363654	6	49	from	media	1460:1464	arg1	concentrations					1413:1426	decreased concentrations	1403:1426	decreased concentrations of yeast extract in the mineral media	1403:1464	Greater EPS yields were achieved with increased concentrations of sodium succinate and sucrose and with decreased concentrations of yeast extract in the mineral media.
28363654	6	50	theme	EPS	1307:1309	arg1	yields					1311:1316	Greater EPS yields	1299:1316	Greater EPS yields	1299:1316	Greater EPS yields were achieved with increased concentrations of sodium succinate and sucrose and with decreased concentrations of yeast extract in the mineral media.
28363654	6	51	from	extract	1437:1443	arg1	media					1460:1464	the mineral media	1448:1464	the mineral media	1448:1464	Greater EPS yields were achieved with increased concentrations of sodium succinate and sucrose and with decreased concentrations of yeast extract in the mineral media.
28363654	1	52	theme	yeast	447:451	arg1	M3					485:486	M3	485:486	M3	485:486	Selected Bacillus strains were investigated for the production of exopolysaccharides (EPS) by fermentation in three different culture media: (i) mineral base-medium with added yeast extract (M1), (ii) succinate-containing mineral base-medium with added yeast extract (M2) and (iii) tryptone and yeast extract-containing base-medium (M3).
28363654	1	52	theme	yeast	447:451	arg1	base-medium					472:482	yeast extract-containing base-medium	447:482	yeast extract-containing base-medium (M3)	447:487	Selected Bacillus strains were investigated for the production of exopolysaccharides (EPS) by fermentation in three different culture media: (i) mineral base-medium with added yeast extract (M1), (ii) succinate-containing mineral base-medium with added yeast extract (M2) and (iii) tryptone and yeast extract-containing base-medium (M3).
28363654	2	53	theme	Modest	490:495	arg1	yield					501:505	Modest EPS yield	490:505	Modest EPS yield of 6.7g/L	490:515	Modest EPS yield of 6.7g/L was recorded for Bacillus amyloliquefaciens 23350 grown on M1, where the EPS produced was characterized by a low MW (<5kDa) and of being mainly glucans.
28363654	3	54	theme	significant	679:689	arg1	yield					691:695	The most significant yield	670:695	The most significant yield (48.57g/L)	670:706	The most significant yield (48.57g/L) was obtained with Bacillus licheniformis 14580 in M2; the EPS produced was 5-30kDa in size and characterized by an exceptionally heterogeneous monosaccharide profile with galactose, fructose and glucose as the predominating monomers.
28363654	3	54	theme	significant	679:689	arg1	48.57g/L					698:705	48.57g/L	698:705	48.57g/L	698:705	The most significant yield (48.57g/L) was obtained with Bacillus licheniformis 14580 in M2; the EPS produced was 5-30kDa in size and characterized by an exceptionally heterogeneous monosaccharide profile with galactose, fructose and glucose as the predominating monomers.
28363654	3	55	dep	Bacillus	726:733	arg1	licheniformis					735:747	licheniformis	735:747	licheniformis	735:747	The most significant yield (48.57g/L) was obtained with Bacillus licheniformis 14580 in M2; the EPS produced was 5-30kDa in size and characterized by an exceptionally heterogeneous monosaccharide profile with galactose, fructose and glucose as the predominating monomers.
28363654	6	56	theme	sucrose	1386:1392	arg1	concentrations					1347:1360	increased concentrations	1337:1360	increased concentrations of sodium succinate and sucrose	1337:1392	Greater EPS yields were achieved with increased concentrations of sodium succinate and sucrose and with decreased concentrations of yeast extract in the mineral media.
28363654	1	57	theme	extract-containing	453:470	arg1	M3					485:486	M3	485:486	M3	485:486	Selected Bacillus strains were investigated for the production of exopolysaccharides (EPS) by fermentation in three different culture media: (i) mineral base-medium with added yeast extract (M1), (ii) succinate-containing mineral base-medium with added yeast extract (M2) and (iii) tryptone and yeast extract-containing base-medium (M3).
28363654	1	57	theme	extract-containing	453:470	arg1	base-medium					472:482	yeast extract-containing base-medium	447:482	yeast extract-containing base-medium (M3)	447:487	Selected Bacillus strains were investigated for the production of exopolysaccharides (EPS) by fermentation in three different culture media: (i) mineral base-medium with added yeast extract (M1), (ii) succinate-containing mineral base-medium with added yeast extract (M2) and (iii) tryptone and yeast extract-containing base-medium (M3).
28363654	5	58	theme	sodium	1164:1169	arg1	succinate					1171:1179	sodium succinate	1164:1179	sodium succinate	1164:1179	The effect of the concentrations of yeast extract, sodium succinate and sucrose on the EPS production by B. licheniformis 14580 were studied using response surface methodology analysis.
28363654	1	59	theme	added	322:326	arg1	M1					343:344	M1	343:344	M1	343:344	Selected Bacillus strains were investigated for the production of exopolysaccharides (EPS) by fermentation in three different culture media: (i) mineral base-medium with added yeast extract (M1), (ii) succinate-containing mineral base-medium with added yeast extract (M2) and (iii) tryptone and yeast extract-containing base-medium (M3).
28363654	1	59	theme	added	322:326	arg1	extract					334:340	added yeast extract	322:340	added yeast extract (M1)	322:345	Selected Bacillus strains were investigated for the production of exopolysaccharides (EPS) by fermentation in three different culture media: (i) mineral base-medium with added yeast extract (M1), (ii) succinate-containing mineral base-medium with added yeast extract (M2) and (iii) tryptone and yeast extract-containing base-medium (M3).
28363654	5	60	from	effect	1117:1122	arg1	production					1204:1213	the EPS production	1196:1213	the EPS production by B. licheniformis 14580	1196:1239	The effect of the concentrations of yeast extract, sodium succinate and sucrose on the EPS production by B. licheniformis 14580 were studied using response surface methodology analysis.
28363654	1	61	theme	added	399:403	arg1	M2					420:421	M2	420:421	M2	420:421	Selected Bacillus strains were investigated for the production of exopolysaccharides (EPS) by fermentation in three different culture media: (i) mineral base-medium with added yeast extract (M1), (ii) succinate-containing mineral base-medium with added yeast extract (M2) and (iii) tryptone and yeast extract-containing base-medium (M3).
28363654	1	61	theme	added	399:403	arg1	extract					411:417	added yeast extract	399:417	added yeast extract (M2)	399:422	Selected Bacillus strains were investigated for the production of exopolysaccharides (EPS) by fermentation in three different culture media: (i) mineral base-medium with added yeast extract (M1), (ii) succinate-containing mineral base-medium with added yeast extract (M2) and (iii) tryptone and yeast extract-containing base-medium (M3).
28363654	3	62	from	size	794:797	arg1	5-30kDa					783:789	5-30kDa	783:789	5-30kDa	783:789	The most significant yield (48.57g/L) was obtained with Bacillus licheniformis 14580 in M2; the EPS produced was 5-30kDa in size and characterized by an exceptionally heterogeneous monosaccharide profile with galactose, fructose and glucose as the predominating monomers.
28363654	5	63	theme	succinate	1171:1179	arg1	concentrations					1131:1144	the concentrations	1127:1144	the concentrations of yeast extract, sodium succinate and sucrose	1127:1191	The effect of the concentrations of yeast extract, sodium succinate and sucrose on the EPS production by B. licheniformis 14580 were studied using response surface methodology analysis.
28363654	2	64	theme	6.7g/L	510:515	arg1	yield					501:505	Modest EPS yield	490:505	Modest EPS yield of 6.7g/L	490:515	Modest EPS yield of 6.7g/L was recorded for Bacillus amyloliquefaciens 23350 grown on M1, where the EPS produced was characterized by a low MW (<5kDa) and of being mainly glucans.
28363654	1	65	theme	yeast	405:409	arg1	M2					420:421	M2	420:421	M2	420:421	Selected Bacillus strains were investigated for the production of exopolysaccharides (EPS) by fermentation in three different culture media: (i) mineral base-medium with added yeast extract (M1), (ii) succinate-containing mineral base-medium with added yeast extract (M2) and (iii) tryptone and yeast extract-containing base-medium (M3).
28363654	1	65	theme	yeast	405:409	arg1	extract					411:417	added yeast extract	399:417	added yeast extract (M2)	399:422	Selected Bacillus strains were investigated for the production of exopolysaccharides (EPS) by fermentation in three different culture media: (i) mineral base-medium with added yeast extract (M1), (ii) succinate-containing mineral base-medium with added yeast extract (M2) and (iii) tryptone and yeast extract-containing base-medium (M3).
28363654	4	66	theme	heteropolymers	1049:1062	arg1	EPS					1064:1066	the obtained heteropolymers EPS	1036:1066	the obtained heteropolymers EPS	1036:1066	The use of M1 medium for the growth of B. licheniformis 14580 resulted in low yield; however, the obtained heteropolymers EPS were characterized by a higher MW 30-100kDa.
28363654	3	67	theme	monosaccharide	851:864	arg1	profile					866:872	an exceptionally heterogeneous monosaccharide profile	820:872	an exceptionally heterogeneous monosaccharide profile with galactose, fructose and glucose	820:909	The most significant yield (48.57g/L) was obtained with Bacillus licheniformis 14580 in M2; the EPS produced was 5-30kDa in size and characterized by an exceptionally heterogeneous monosaccharide profile with galactose, fructose and glucose as the predominating monomers.
28363654	4	68	theme	14580	998:1002	arg1	growth					971:976	the growth	967:976	the growth of B. licheniformis 14580	967:1002	The use of M1 medium for the growth of B. licheniformis 14580 resulted in low yield; however, the obtained heteropolymers EPS were characterized by a higher MW 30-100kDa.
28363654	1	69	from	production	204:213	arg1	media					286:290	three different culture media	262:290	three different culture media	262:290	Selected Bacillus strains were investigated for the production of exopolysaccharides (EPS) by fermentation in three different culture media: (i) mineral base-medium with added yeast extract (M1), (ii) succinate-containing mineral base-medium with added yeast extract (M2) and (iii) tryptone and yeast extract-containing base-medium (M3).
28363654	6	70	theme	Greater	1299:1305	arg1	yields					1311:1316	Greater EPS yields	1299:1316	Greater EPS yields	1299:1316	Greater EPS yields were achieved with increased concentrations of sodium succinate and sucrose and with decreased concentrations of yeast extract in the mineral media.
28363654	5	71	theme	sucrose	1185:1191	arg1	concentrations					1131:1144	the concentrations	1127:1144	the concentrations of yeast extract, sodium succinate and sucrose	1127:1191	The effect of the concentrations of yeast extract, sodium succinate and sucrose on the EPS production by B. licheniformis 14580 were studied using response surface methodology analysis.
28363654	3	72	theme	Bacillus	726:733	arg1	14580					749:753	Bacillus licheniformis 14580	726:753	Bacillus licheniformis 14580 in M2	726:759	The most significant yield (48.57g/L) was obtained with Bacillus licheniformis 14580 in M2; the EPS produced was 5-30kDa in size and characterized by an exceptionally heterogeneous monosaccharide profile with galactose, fructose and glucose as the predominating monomers.
28363654	1	73	theme	yeast	328:332	arg1	M1					343:344	M1	343:344	M1	343:344	Selected Bacillus strains were investigated for the production of exopolysaccharides (EPS) by fermentation in three different culture media: (i) mineral base-medium with added yeast extract (M1), (ii) succinate-containing mineral base-medium with added yeast extract (M2) and (iii) tryptone and yeast extract-containing base-medium (M3).
28363654	1	73	theme	yeast	328:332	arg1	extract					334:340	added yeast extract	322:340	added yeast extract (M1)	322:345	Selected Bacillus strains were investigated for the production of exopolysaccharides (EPS) by fermentation in three different culture media: (i) mineral base-medium with added yeast extract (M1), (ii) succinate-containing mineral base-medium with added yeast extract (M2) and (iii) tryptone and yeast extract-containing base-medium (M3).
28363654	0	74	dep	Production	0:9	arg1	Optimization					63:74	Optimization	63:74	Production of exopolysaccharides by selected Bacillus strains: Optimization of media composition to maximize the yield and structural characterization.	0:150	Production of exopolysaccharides by selected Bacillus strains: Optimization of media composition to maximize the yield and structural characterization.
28363654	1	75	theme	Bacillus	161:168	arg1	strains					170:176	Bacillus strains	161:176	Bacillus strains	161:176	Selected Bacillus strains were investigated for the production of exopolysaccharides (EPS) by fermentation in three different culture media: (i) mineral base-medium with added yeast extract (M1), (ii) succinate-containing mineral base-medium with added yeast extract (M2) and (iii) tryptone and yeast extract-containing base-medium (M3).
28363654	5	76	theme	concentrations	1131:1144	arg1	effect					1117:1122	The effect	1113:1122	The effect of the concentrations of yeast extract, sodium succinate and sucrose on the EPS production by B. licheniformis 14580	1113:1239	The effect of the concentrations of yeast extract, sodium succinate and sucrose on the EPS production by B. licheniformis 14580 were studied using response surface methodology analysis.
28464099	10	0	from	EPL	1766:1768	arg1	pigs					1785:1788	pigs	1785:1788	pigs	1785:1788	The results of this study indicate that EPL (mg/kg DMI) in pigs substantially increase with increasing BW.
28464099	9	1	theme	INU	1613:1615	arg1	diet					1617:1620	the INU diet	1609:1620	the INU diet	1609:1620	When expressed in mg/(kg BW·d), however, P excretion was similar in GF pigs and sows that were fed the STA diet and in those that were fed the INU diet, whereas it was greater in sows than in GF pigs that were fed the CEL diet (11.6 vs. 7.3 mg/(kg BW·d)).
28464099	12	2	theme	increasing	2010:2019	arg1	content					2021:2027	an increasing content	2007:2027	an increasing content of dietary nonstarch polysaccharides (NSP)	2007:2070	Moreover, EPL is diet dependent and increases with an increasing content of dietary nonstarch polysaccharides (NSP).
28464099	3	3	dep	composition	519:529	arg1	INU					569:571	INU	569:571	INU	569:571	Treatments were animal type (GF pigs or sows), diet composition (a semipurified starch (STA), inulin (INU), or lignocellulose (CEL) based low-P diet), and FL (2.0 or 3.0 kg/d).
28464099	3	3	dep	composition	519:529	arg1	STA					555:557	STA	555:557	STA	555:557	Treatments were animal type (GF pigs or sows), diet composition (a semipurified starch (STA), inulin (INU), or lignocellulose (CEL) based low-P diet), and FL (2.0 or 3.0 kg/d).
28464099	3	3	dep	composition	519:529	arg1	inulin					561:566	inulin	561:566	inulin (INU)	561:572	Treatments were animal type (GF pigs or sows), diet composition (a semipurified starch (STA), inulin (INU), or lignocellulose (CEL) based low-P diet), and FL (2.0 or 3.0 kg/d).
28464099	3	3	dep	composition	519:529	arg1	starch					547:552	a semipurified starch	532:552	a semipurified starch (STA)	532:558	Treatments were animal type (GF pigs or sows), diet composition (a semipurified starch (STA), inulin (INU), or lignocellulose (CEL) based low-P diet), and FL (2.0 or 3.0 kg/d).
28464099	3	3	dep	composition	519:529	arg1	diet					611:614	lignocellulose (CEL) based low-P diet	578:614	lignocellulose (CEL) based low-P diet	578:614	Treatments were animal type (GF pigs or sows), diet composition (a semipurified starch (STA), inulin (INU), or lignocellulose (CEL) based low-P diet), and FL (2.0 or 3.0 kg/d).
28464099	7	4	theme	P	1251:1251	arg1	g/d					1264:1266	g/d	1264:1266	g/d	1264:1266	Both BW and FL increased fecal P excretion (g/d).
28464099	7	4	theme	P	1251:1251	arg1	excretion					1253:1261	fecal P excretion	1245:1261	fecal P excretion (g/d)	1245:1267	Both BW and FL increased fecal P excretion (g/d).
28464099	11	5	theme	GF	1878:1879	arg1	pigs					1881:1884	GF pigs	1878:1884	GF pigs	1878:1884	Application of EPL (mg/kg DMI) determined in GF pigs may underestimate EPL and therefore P requirements in gestating sows.
28464099	0	6	theme	Endogenous	0:9	arg1	losses					22:27	Endogenous phosphorus losses	0:27	Endogenous phosphorus losses in growing-finishing pigs and gestating sows	0:72	Endogenous phosphorus losses in growing-finishing pigs and gestating sows.
28464099	9	7	dep	diet	1692:1695	arg1	mg/					1711:1713	11.6 vs. 7.3 mg/	1698:1713	11.6 vs. 7.3 mg/(kg BW·d)	1698:1722	When expressed in mg/(kg BW·d), however, P excretion was similar in GF pigs and sows that were fed the STA diet and in those that were fed the INU diet, whereas it was greater in sows than in GF pigs that were fed the CEL diet (11.6 vs. 7.3 mg/(kg BW·d)).
28464099	9	7	dep	diet	1692:1695	arg1	BW·d					1718:1721	kg BW·d	1715:1721	kg BW·d	1715:1721	When expressed in mg/(kg BW·d), however, P excretion was similar in GF pigs and sows that were fed the STA diet and in those that were fed the INU diet, whereas it was greater in sows than in GF pigs that were fed the CEL diet (11.6 vs. 7.3 mg/(kg BW·d)).
28464099	9	8	from	pigs	1541:1544	arg1	similar					1527:1533	similar	1527:1533	similar	1527:1533	When expressed in mg/(kg BW·d), however, P excretion was similar in GF pigs and sows that were fed the STA diet and in those that were fed the INU diet, whereas it was greater in sows than in GF pigs that were fed the CEL diet (11.6 vs. 7.3 mg/(kg BW·d)).
28464099	6	9	from	sows	1021:1024	arg1	similar					1010:1016	similar	1010:1016	similar	1010:1016	While digestibility of OM and COH was similar in sows and GF pigs that were fed the STA diet and the CEL diet, on the INU diet, sows had, compared with GF pigs, a greater digestibility of OM (92.2 vs. 87.2%) and COH (92.5 vs. 88.4%), respectively.
28464099	2	10	theme	GF	290:291	arg1	kg					315:316	initial BW 90.5 kg	299:316	initial BW 90.5 kg	299:316	After an adaptation period, 48 GF pigs (initial BW 90.5 kg) and 48 just-weaned sows (initial BW 195 kg), both individually housed, were allotted to 12 dietary treatments in a 2 × 3 × 2 factorial arrangement.
28464099	2	10	theme	GF	290:291	arg1	pigs					293:296	48 GF pigs	287:296	48 GF pigs (initial BW 90.5 kg)	287:317	After an adaptation period, 48 GF pigs (initial BW 90.5 kg) and 48 just-weaned sows (initial BW 195 kg), both individually housed, were allotted to 12 dietary treatments in a 2 × 3 × 2 factorial arrangement.
28464099	6	11	theme	CEL	1073:1075	arg1	diet					1077:1080	the CEL diet	1069:1080	the CEL diet	1069:1080	While digestibility of OM and COH was similar in sows and GF pigs that were fed the STA diet and the CEL diet, on the INU diet, sows had, compared with GF pigs, a greater digestibility of OM (92.2 vs. 87.2%) and COH (92.5 vs. 88.4%), respectively.
28464099	1	12	theme	BW	173:174	arg1	effects					120:126	the effects	116:126	the effects of diet composition, feeding level (FL), and BW on endogenous phosphorous losses (EPL) using growing-finishing (GF) pigs and sows	116:256	An experiment was conducted to determine the effects of diet composition, feeding level (FL), and BW on endogenous phosphorous losses (EPL) using growing-finishing (GF) pigs and sows.
28464099	13	13	theme	fiber	2182:2186	arg1	properties					2160:2169	properties	2160:2169	properties of dietary fiber	2160:2186	The degree of this increase may differ between sows and GF pigs and seems to depend on properties of dietary fiber.
28464099	3	14	theme	lignocellulose	578:591	arg1	diet					611:614	lignocellulose (CEL) based low-P diet	578:614	lignocellulose (CEL) based low-P diet	578:614	Treatments were animal type (GF pigs or sows), diet composition (a semipurified starch (STA), inulin (INU), or lignocellulose (CEL) based low-P diet), and FL (2.0 or 3.0 kg/d).
28464099	1	15	theme	endogenous	179:188	arg1	EPL					210:212	EPL	210:212	EPL	210:212	An experiment was conducted to determine the effects of diet composition, feeding level (FL), and BW on endogenous phosphorous losses (EPL) using growing-finishing (GF) pigs and sows.
28464099	1	15	theme	endogenous	179:188	arg1	losses					202:207	endogenous phosphorous losses	179:207	endogenous phosphorous losses (EPL) using growing-finishing (GF) pigs and sows	179:256	An experiment was conducted to determine the effects of diet composition, feeding level (FL), and BW on endogenous phosphorous losses (EPL) using growing-finishing (GF) pigs and sows.
28464099	6	16	contain	had	1105:1107	arg2	COH					1184:1186	COH	1184:1186	COH (92.5 vs. 88.4%)	1184:1203	While digestibility of OM and COH was similar in sows and GF pigs that were fed the STA diet and the CEL diet, on the INU diet, sows had, compared with GF pigs, a greater digestibility of OM (92.2 vs. 87.2%) and COH (92.5 vs. 88.4%), respectively.
28464099	6	16	contain	had	1105:1107	arg2	%					1177:1177	92.2 vs. 87.2%	1164:1177	92.2 vs. 87.2%	1164:1177	While digestibility of OM and COH was similar in sows and GF pigs that were fed the STA diet and the CEL diet, on the INU diet, sows had, compared with GF pigs, a greater digestibility of OM (92.2 vs. 87.2%) and COH (92.5 vs. 88.4%), respectively.
28464099	6	16	contain	had	1105:1107	arg2	digestibility					1143:1155	a greater digestibility	1133:1155	a greater digestibility of OM (92.2 vs. 87.2%)	1133:1178	While digestibility of OM and COH was similar in sows and GF pigs that were fed the STA diet and the CEL diet, on the INU diet, sows had, compared with GF pigs, a greater digestibility of OM (92.2 vs. 87.2%) and COH (92.5 vs. 88.4%), respectively.
28464099	6	16	contain	had	1105:1107	arg1	sows					1100:1103	sows	1100:1103	sows	1100:1103	While digestibility of OM and COH was similar in sows and GF pigs that were fed the STA diet and the CEL diet, on the INU diet, sows had, compared with GF pigs, a greater digestibility of OM (92.2 vs. 87.2%) and COH (92.5 vs. 88.4%), respectively.
28464099	6	16	contain	had	1105:1107	arg2	%					1202:1202	92.5 vs. 88.4%	1189:1202	%	1202:1202	While digestibility of OM and COH was similar in sows and GF pigs that were fed the STA diet and the CEL diet, on the INU diet, sows had, compared with GF pigs, a greater digestibility of OM (92.2 vs. 87.2%) and COH (92.5 vs. 88.4%), respectively.
28464099	8	17	theme	mg/kg	1288:1292	arg1	DMI					1294:1296	mg/kg DMI	1288:1296	mg/kg DMI	1288:1296	When expressed in mg/kg DMI, P excretion was higher in sows than in GF pigs on the STA diet (498 versus 236 mg/kg DMI), the INU diet (526 vs. 316 mg/kg DMI), and the CEL diet (928 vs. 342 mg/kg DMI).
28464099	3	18	theme	based	599:603	arg1	diet					611:614	lignocellulose (CEL) based low-P diet	578:614	lignocellulose (CEL) based low-P diet	578:614	Treatments were animal type (GF pigs or sows), diet composition (a semipurified starch (STA), inulin (INU), or lignocellulose (CEL) based low-P diet), and FL (2.0 or 3.0 kg/d).
28464099	6	19	from	similar	1010:1016	arg1	sows					1021:1024	sows	1021:1024	sows	1021:1024	While digestibility of OM and COH was similar in sows and GF pigs that were fed the STA diet and the CEL diet, on the INU diet, sows had, compared with GF pigs, a greater digestibility of OM (92.2 vs. 87.2%) and COH (92.5 vs. 88.4%), respectively.
28464099	6	19	from	similar	1010:1016	arg1	pigs					1033:1036	GF pigs	1030:1036	GF pigs	1030:1036	While digestibility of OM and COH was similar in sows and GF pigs that were fed the STA diet and the CEL diet, on the INU diet, sows had, compared with GF pigs, a greater digestibility of OM (92.2 vs. 87.2%) and COH (92.5 vs. 88.4%), respectively.
28464099	1	20	theme	diet	131:134	arg1	composition					136:146	diet composition	131:146	diet composition	131:146	An experiment was conducted to determine the effects of diet composition, feeding level (FL), and BW on endogenous phosphorous losses (EPL) using growing-finishing (GF) pigs and sows.
28464099	13	21	theme	GF	2129:2130	arg1	pigs					2132:2135	GF pigs	2129:2135	GF pigs	2129:2135	The degree of this increase may differ between sows and GF pigs and seems to depend on properties of dietary fiber.
28464099	4	22	theme	indigestible	797:808	arg1	marker					810:815	indigestible marker	797:815	indigestible marker	797:815	Digestibility of DM, OM, CP, crude fat, and carbohydrates (COH), and fecal P excretion (in g/d, mg/kg DMI, and g/(kg BW·d)) were determined using TiO as indigestible marker.
28464099	3	23	theme	CEL	594:596	arg1	diet					611:614	lignocellulose (CEL) based low-P diet	578:614	lignocellulose (CEL) based low-P diet	578:614	Treatments were animal type (GF pigs or sows), diet composition (a semipurified starch (STA), inulin (INU), or lignocellulose (CEL) based low-P diet), and FL (2.0 or 3.0 kg/d).
28464099	4	24	from	fat	679:681	arg1	DMI					746:748	mg/kg DMI	740:748	mg/kg DMI	740:748	Digestibility of DM, OM, CP, crude fat, and carbohydrates (COH), and fecal P excretion (in g/d, mg/kg DMI, and g/(kg BW·d)) were determined using TiO as indigestible marker.
28464099	4	24	from	fat	679:681	arg1	g/					755:756	g/	755:756	g/(kg BW·d)	755:765	Digestibility of DM, OM, CP, crude fat, and carbohydrates (COH), and fecal P excretion (in g/d, mg/kg DMI, and g/(kg BW·d)) were determined using TiO as indigestible marker.
28464099	4	24	from	fat	679:681	arg1	BW·d					761:764	kg BW·d	758:764	kg BW·d	758:764	Digestibility of DM, OM, CP, crude fat, and carbohydrates (COH), and fecal P excretion (in g/d, mg/kg DMI, and g/(kg BW·d)) were determined using TiO as indigestible marker.
28464099	4	24	from	fat	679:681	arg1	g/d					735:737	g/d	735:737	g/d	735:737	Digestibility of DM, OM, CP, crude fat, and carbohydrates (COH), and fecal P excretion (in g/d, mg/kg DMI, and g/(kg BW·d)) were determined using TiO as indigestible marker.
28464099	3	25	theme	animal	483:488	arg1	type					490:493	animal type	483:493	animal type (GF pigs or sows)	483:511	Treatments were animal type (GF pigs or sows), diet composition (a semipurified starch (STA), inulin (INU), or lignocellulose (CEL) based low-P diet), and FL (2.0 or 3.0 kg/d).
28464099	0	26	from	losses	22:27	arg1	pigs					50:53	growing-finishing pigs	32:53	growing-finishing pigs	32:53	Endogenous phosphorus losses in growing-finishing pigs and gestating sows.
28464099	0	26	from	losses	22:27	arg1	sows					69:72	gestating sows	59:72	gestating sows	59:72	Endogenous phosphorus losses in growing-finishing pigs and gestating sows.
28464099	2	27	theme	×	436:436	arg1	arrangement					454:464	a 2 × 3 × 2 factorial arrangement	432:464	a 2 × 3 × 2 factorial arrangement	432:464	After an adaptation period, 48 GF pigs (initial BW 90.5 kg) and 48 just-weaned sows (initial BW 195 kg), both individually housed, were allotted to 12 dietary treatments in a 2 × 3 × 2 factorial arrangement.
28464099	1	28	theme	level	157:161	arg1	effects					120:126	the effects	116:126	the effects of diet composition, feeding level (FL), and BW on endogenous phosphorous losses (EPL) using growing-finishing (GF) pigs and sows	116:256	An experiment was conducted to determine the effects of diet composition, feeding level (FL), and BW on endogenous phosphorous losses (EPL) using growing-finishing (GF) pigs and sows.
28464099	2	29	theme	dietary	410:416	arg1	treatments					418:427	12 dietary treatments	407:427	12 dietary treatments in a 2 × 3 × 2 factorial arrangement	407:464	After an adaptation period, 48 GF pigs (initial BW 90.5 kg) and 48 just-weaned sows (initial BW 195 kg), both individually housed, were allotted to 12 dietary treatments in a 2 × 3 × 2 factorial arrangement.
28464099	9	30	theme	GF	1538:1539	arg1	pigs					1541:1544	GF pigs	1538:1544	GF pigs	1538:1544	When expressed in mg/(kg BW·d), however, P excretion was similar in GF pigs and sows that were fed the STA diet and in those that were fed the INU diet, whereas it was greater in sows than in GF pigs that were fed the CEL diet (11.6 vs. 7.3 mg/(kg BW·d)).
28464099	6	31	theme	OM	1160:1161	arg1	%					1202:1202	92.5 vs. 88.4%	1189:1202	%	1202:1202	While digestibility of OM and COH was similar in sows and GF pigs that were fed the STA diet and the CEL diet, on the INU diet, sows had, compared with GF pigs, a greater digestibility of OM (92.2 vs. 87.2%) and COH (92.5 vs. 88.4%), respectively.
28464099	6	31	theme	OM	1160:1161	arg1	COH					1184:1186	COH	1184:1186	COH (92.5 vs. 88.4%)	1184:1203	While digestibility of OM and COH was similar in sows and GF pigs that were fed the STA diet and the CEL diet, on the INU diet, sows had, compared with GF pigs, a greater digestibility of OM (92.2 vs. 87.2%) and COH (92.5 vs. 88.4%), respectively.
28464099	6	31	theme	OM	1160:1161	arg1	%					1177:1177	92.2 vs. 87.2%	1164:1177	92.2 vs. 87.2%	1164:1177	While digestibility of OM and COH was similar in sows and GF pigs that were fed the STA diet and the CEL diet, on the INU diet, sows had, compared with GF pigs, a greater digestibility of OM (92.2 vs. 87.2%) and COH (92.5 vs. 88.4%), respectively.
28464099	6	31	theme	OM	1160:1161	arg1	digestibility					1143:1155	a greater digestibility	1133:1155	a greater digestibility of OM (92.2 vs. 87.2%)	1133:1178	While digestibility of OM and COH was similar in sows and GF pigs that were fed the STA diet and the CEL diet, on the INU diet, sows had, compared with GF pigs, a greater digestibility of OM (92.2 vs. 87.2%) and COH (92.5 vs. 88.4%), respectively.
28464099	10	32	theme	study	1746:1750	arg1	results					1730:1736	The results	1726:1736	The results of this study	1726:1750	The results of this study indicate that EPL (mg/kg DMI) in pigs substantially increase with increasing BW.
28464099	9	33	from	those	1589:1593	arg1	similar					1527:1533	similar	1527:1533	similar	1527:1533	When expressed in mg/(kg BW·d), however, P excretion was similar in GF pigs and sows that were fed the STA diet and in those that were fed the INU diet, whereas it was greater in sows than in GF pigs that were fed the CEL diet (11.6 vs. 7.3 mg/(kg BW·d)).
28464099	3	34	theme	low-P	605:609	arg1	diet					611:614	lignocellulose (CEL) based low-P diet	578:614	lignocellulose (CEL) based low-P diet	578:614	Treatments were animal type (GF pigs or sows), diet composition (a semipurified starch (STA), inulin (INU), or lignocellulose (CEL) based low-P diet), and FL (2.0 or 3.0 kg/d).
28464099	5	35	theme	COH	842:844	arg1	Digestibility					818:830	Digestibility	818:830	Digestibility of OM and COH	818:844	Digestibility of OM and COH differed among diets ( < 0.001) and was greatest in both types of pigs fed the STA diet and lowest in those fed the CEL diet.
28464099	8	36	theme	INU	1394:1396	arg1	diet					1398:1401	the INU diet	1390:1401	the INU diet (526 vs. 316 mg/kg DMI)	1390:1425	When expressed in mg/kg DMI, P excretion was higher in sows than in GF pigs on the STA diet (498 versus 236 mg/kg DMI), the INU diet (526 vs. 316 mg/kg DMI), and the CEL diet (928 vs. 342 mg/kg DMI).
28464099	8	36	theme	INU	1394:1396	arg1	DMI					1422:1424	526 vs. 316 mg/kg DMI	1404:1424	526 vs. 316 mg/kg DMI	1404:1424	When expressed in mg/kg DMI, P excretion was higher in sows than in GF pigs on the STA diet (498 versus 236 mg/kg DMI), the INU diet (526 vs. 316 mg/kg DMI), and the CEL diet (928 vs. 342 mg/kg DMI).
28464099	1	37	theme	GF	240:241	arg1	pigs					244:247	growing-finishing (GF) pigs	221:247	growing-finishing (GF) pigs	221:247	An experiment was conducted to determine the effects of diet composition, feeding level (FL), and BW on endogenous phosphorous losses (EPL) using growing-finishing (GF) pigs and sows.
28464099	2	38	theme	initial	344:350	arg1	kg					359:360	initial BW 195 kg	344:360	initial BW 195 kg	344:360	After an adaptation period, 48 GF pigs (initial BW 90.5 kg) and 48 just-weaned sows (initial BW 195 kg), both individually housed, were allotted to 12 dietary treatments in a 2 × 3 × 2 factorial arrangement.
28464099	2	38	theme	initial	344:350	arg1	sows					338:341	48 just-weaned sows	323:341	48 just-weaned sows (initial BW 195 kg)	323:361	After an adaptation period, 48 GF pigs (initial BW 90.5 kg) and 48 just-weaned sows (initial BW 195 kg), both individually housed, were allotted to 12 dietary treatments in a 2 × 3 × 2 factorial arrangement.
28464099	5	39	theme	STA	925:927	arg1	diet					929:932	the STA diet	921:932	the STA diet	921:932	Digestibility of OM and COH differed among diets ( < 0.001) and was greatest in both types of pigs fed the STA diet and lowest in those fed the CEL diet.
28464099	8	40	theme	CEL	1436:1438	arg1	diet					1440:1443	the CEL diet	1432:1443	the CEL diet (928 vs. 342 mg/kg DMI)	1432:1467	When expressed in mg/kg DMI, P excretion was higher in sows than in GF pigs on the STA diet (498 versus 236 mg/kg DMI), the INU diet (526 vs. 316 mg/kg DMI), and the CEL diet (928 vs. 342 mg/kg DMI).
28464099	8	40	theme	CEL	1436:1438	arg1	DMI					1464:1466	928 vs. 342 mg/kg DMI	1446:1466	928 vs. 342 mg/kg DMI	1446:1466	When expressed in mg/kg DMI, P excretion was higher in sows than in GF pigs on the STA diet (498 versus 236 mg/kg DMI), the INU diet (526 vs. 316 mg/kg DMI), and the CEL diet (928 vs. 342 mg/kg DMI).
28464099	9	41	theme	STA	1573:1575	arg1	diet					1577:1580	the STA diet	1569:1580	the STA diet	1569:1580	When expressed in mg/(kg BW·d), however, P excretion was similar in GF pigs and sows that were fed the STA diet and in those that were fed the INU diet, whereas it was greater in sows than in GF pigs that were fed the CEL diet (11.6 vs. 7.3 mg/(kg BW·d)).
28464099	0	42	theme	growing-finishing	32:48	arg1	pigs					50:53	growing-finishing pigs	32:53	growing-finishing pigs	32:53	Endogenous phosphorus losses in growing-finishing pigs and gestating sows.
28464099	4	43	theme	P	719:719	arg1	excretion					721:729	fecal P excretion	713:729	fecal P excretion (in g/d, mg/kg DMI, and g/(kg BW·d))	713:766	Digestibility of DM, OM, CP, crude fat, and carbohydrates (COH), and fecal P excretion (in g/d, mg/kg DMI, and g/(kg BW·d)) were determined using TiO as indigestible marker.
28464099	12	44	theme	nonstarch	2040:2048	arg1	NSP					2067:2069	NSP	2067:2069	NSP	2067:2069	Moreover, EPL is diet dependent and increases with an increasing content of dietary nonstarch polysaccharides (NSP).
28464099	12	44	theme	nonstarch	2040:2048	arg1	polysaccharides					2050:2064	dietary nonstarch polysaccharides	2032:2064	dietary nonstarch polysaccharides (NSP)	2032:2070	Moreover, EPL is diet dependent and increases with an increasing content of dietary nonstarch polysaccharides (NSP).
28464099	8	45	theme	mg/kg	1416:1420	arg1	diet					1398:1401	the INU diet	1390:1401	the INU diet (526 vs. 316 mg/kg DMI)	1390:1425	When expressed in mg/kg DMI, P excretion was higher in sows than in GF pigs on the STA diet (498 versus 236 mg/kg DMI), the INU diet (526 vs. 316 mg/kg DMI), and the CEL diet (928 vs. 342 mg/kg DMI).
28464099	8	45	theme	mg/kg	1416:1420	arg1	DMI					1422:1424	526 vs. 316 mg/kg DMI	1404:1424	526 vs. 316 mg/kg DMI	1404:1424	When expressed in mg/kg DMI, P excretion was higher in sows than in GF pigs on the STA diet (498 versus 236 mg/kg DMI), the INU diet (526 vs. 316 mg/kg DMI), and the CEL diet (928 vs. 342 mg/kg DMI).
28464099	2	46	dep	individually	369:380	arg1	housed					382:387	housed	382:387	housed	382:387	After an adaptation period, 48 GF pigs (initial BW 90.5 kg) and 48 just-weaned sows (initial BW 195 kg), both individually housed, were allotted to 12 dietary treatments in a 2 × 3 × 2 factorial arrangement.
28464099	1	47	theme	phosphorous	190:200	arg1	EPL					210:212	EPL	210:212	EPL	210:212	An experiment was conducted to determine the effects of diet composition, feeding level (FL), and BW on endogenous phosphorous losses (EPL) using growing-finishing (GF) pigs and sows.
28464099	1	47	theme	phosphorous	190:200	arg1	losses					202:207	endogenous phosphorous losses	179:207	endogenous phosphorous losses (EPL) using growing-finishing (GF) pigs and sows	179:256	An experiment was conducted to determine the effects of diet composition, feeding level (FL), and BW on endogenous phosphorous losses (EPL) using growing-finishing (GF) pigs and sows.
28464099	6	48	from	pigs	1033:1036	arg1	similar					1010:1016	similar	1010:1016	similar	1010:1016	While digestibility of OM and COH was similar in sows and GF pigs that were fed the STA diet and the CEL diet, on the INU diet, sows had, compared with GF pigs, a greater digestibility of OM (92.2 vs. 87.2%) and COH (92.5 vs. 88.4%), respectively.
28464099	8	49	theme	mg/kg	1458:1462	arg1	diet					1440:1443	the CEL diet	1432:1443	the CEL diet (928 vs. 342 mg/kg DMI)	1432:1467	When expressed in mg/kg DMI, P excretion was higher in sows than in GF pigs on the STA diet (498 versus 236 mg/kg DMI), the INU diet (526 vs. 316 mg/kg DMI), and the CEL diet (928 vs. 342 mg/kg DMI).
28464099	8	49	theme	mg/kg	1458:1462	arg1	DMI					1464:1466	928 vs. 342 mg/kg DMI	1446:1466	928 vs. 342 mg/kg DMI	1446:1466	When expressed in mg/kg DMI, P excretion was higher in sows than in GF pigs on the STA diet (498 versus 236 mg/kg DMI), the INU diet (526 vs. 316 mg/kg DMI), and the CEL diet (928 vs. 342 mg/kg DMI).
28464099	8	50	from	pigs	1341:1344	arg1	diet					1357:1360	the STA diet	1349:1360	the STA diet (498 versus 236 mg/kg DMI)	1349:1387	When expressed in mg/kg DMI, P excretion was higher in sows than in GF pigs on the STA diet (498 versus 236 mg/kg DMI), the INU diet (526 vs. 316 mg/kg DMI), and the CEL diet (928 vs. 342 mg/kg DMI).
28464099	8	50	from	pigs	1341:1344	arg1	DMI					1384:1386	498 versus 236 mg/kg DMI	1363:1386	498 versus 236 mg/kg DMI	1363:1386	When expressed in mg/kg DMI, P excretion was higher in sows than in GF pigs on the STA diet (498 versus 236 mg/kg DMI), the INU diet (526 vs. 316 mg/kg DMI), and the CEL diet (928 vs. 342 mg/kg DMI).
28464099	8	50	from	pigs	1341:1344	arg1	DMI					1464:1466	928 vs. 342 mg/kg DMI	1446:1466	928 vs. 342 mg/kg DMI	1446:1466	When expressed in mg/kg DMI, P excretion was higher in sows than in GF pigs on the STA diet (498 versus 236 mg/kg DMI), the INU diet (526 vs. 316 mg/kg DMI), and the CEL diet (928 vs. 342 mg/kg DMI).
28464099	8	50	from	pigs	1341:1344	arg1	DMI					1422:1424	526 vs. 316 mg/kg DMI	1404:1424	526 vs. 316 mg/kg DMI	1404:1424	When expressed in mg/kg DMI, P excretion was higher in sows than in GF pigs on the STA diet (498 versus 236 mg/kg DMI), the INU diet (526 vs. 316 mg/kg DMI), and the CEL diet (928 vs. 342 mg/kg DMI).
28464099	8	50	from	pigs	1341:1344	arg1	diet					1440:1443	the CEL diet	1432:1443	the CEL diet (928 vs. 342 mg/kg DMI)	1432:1467	When expressed in mg/kg DMI, P excretion was higher in sows than in GF pigs on the STA diet (498 versus 236 mg/kg DMI), the INU diet (526 vs. 316 mg/kg DMI), and the CEL diet (928 vs. 342 mg/kg DMI).
28464099	8	50	from	pigs	1341:1344	arg1	diet					1398:1401	the INU diet	1390:1401	the INU diet (526 vs. 316 mg/kg DMI)	1390:1425	When expressed in mg/kg DMI, P excretion was higher in sows than in GF pigs on the STA diet (498 versus 236 mg/kg DMI), the INU diet (526 vs. 316 mg/kg DMI), and the CEL diet (928 vs. 342 mg/kg DMI).
28464099	11	51	theme	gestating	1940:1948	arg1	sows					1950:1953	gestating sows	1940:1953	gestating sows	1940:1953	Application of EPL (mg/kg DMI) determined in GF pigs may underestimate EPL and therefore P requirements in gestating sows.
28464099	5	52	theme	CEL	962:964	arg1	diet					966:969	the CEL diet	958:969	the CEL diet	958:969	Digestibility of OM and COH differed among diets ( < 0.001) and was greatest in both types of pigs fed the STA diet and lowest in those fed the CEL diet.
28464099	2	53	theme	initial	299:305	arg1	kg					315:316	initial BW 90.5 kg	299:316	initial BW 90.5 kg	299:316	After an adaptation period, 48 GF pigs (initial BW 90.5 kg) and 48 just-weaned sows (initial BW 195 kg), both individually housed, were allotted to 12 dietary treatments in a 2 × 3 × 2 factorial arrangement.
28464099	2	53	theme	initial	299:305	arg1	pigs					293:296	48 GF pigs	287:296	48 GF pigs (initial BW 90.5 kg)	287:317	After an adaptation period, 48 GF pigs (initial BW 90.5 kg) and 48 just-weaned sows (initial BW 195 kg), both individually housed, were allotted to 12 dietary treatments in a 2 × 3 × 2 factorial arrangement.
28464099	6	54	theme	COH	1002:1004	arg1	digestibility					978:990	digestibility	978:990	digestibility of OM and COH	978:1004	While digestibility of OM and COH was similar in sows and GF pigs that were fed the STA diet and the CEL diet, on the INU diet, sows had, compared with GF pigs, a greater digestibility of OM (92.2 vs. 87.2%) and COH (92.5 vs. 88.4%), respectively.
28464099	4	55	theme	mg/kg	740:744	arg1	DMI					746:748	mg/kg DMI	740:748	mg/kg DMI	740:748	Digestibility of DM, OM, CP, crude fat, and carbohydrates (COH), and fecal P excretion (in g/d, mg/kg DMI, and g/(kg BW·d)) were determined using TiO as indigestible marker.
28464099	8	56	theme	P	1299:1299	arg1	excretion					1301:1309	P excretion	1299:1309	P excretion	1299:1309	When expressed in mg/kg DMI, P excretion was higher in sows than in GF pigs on the STA diet (498 versus 236 mg/kg DMI), the INU diet (526 vs. 316 mg/kg DMI), and the CEL diet (928 vs. 342 mg/kg DMI).
28464099	1	57	theme	growing-finishing	221:237	arg1	pigs					244:247	growing-finishing (GF) pigs	221:247	growing-finishing (GF) pigs	221:247	An experiment was conducted to determine the effects of diet composition, feeding level (FL), and BW on endogenous phosphorous losses (EPL) using growing-finishing (GF) pigs and sows.
28464099	2	58	theme	factorial	444:452	arg1	arrangement					454:464	a 2 × 3 × 2 factorial arrangement	432:464	a 2 × 3 × 2 factorial arrangement	432:464	After an adaptation period, 48 GF pigs (initial BW 90.5 kg) and 48 just-weaned sows (initial BW 195 kg), both individually housed, were allotted to 12 dietary treatments in a 2 × 3 × 2 factorial arrangement.
28464099	7	59	theme	fecal	1245:1249	arg1	g/d					1264:1266	g/d	1264:1266	g/d	1264:1266	Both BW and FL increased fecal P excretion (g/d).
28464099	7	59	theme	fecal	1245:1249	arg1	excretion					1253:1261	fecal P excretion	1245:1261	fecal P excretion (g/d)	1245:1267	Both BW and FL increased fecal P excretion (g/d).
28464099	6	60	theme	INU	1090:1092	arg1	diet					1094:1097	the INU diet	1086:1097	the INU diet	1086:1097	While digestibility of OM and COH was similar in sows and GF pigs that were fed the STA diet and the CEL diet, on the INU diet, sows had, compared with GF pigs, a greater digestibility of OM (92.2 vs. 87.2%) and COH (92.5 vs. 88.4%), respectively.
28464099	8	61	theme	GF	1338:1339	arg1	pigs					1341:1344	GF pigs	1338:1344	GF pigs on the STA diet (498 versus 236 mg/kg DMI), the INU diet (526 vs. 316 mg/kg DMI), and the CEL diet (928 vs. 342 mg/kg DMI)	1338:1467	When expressed in mg/kg DMI, P excretion was higher in sows than in GF pigs on the STA diet (498 versus 236 mg/kg DMI), the INU diet (526 vs. 316 mg/kg DMI), and the CEL diet (928 vs. 342 mg/kg DMI).
28464099	13	62	theme	dietary	2174:2180	arg1	fiber					2182:2186	dietary fiber	2174:2186	dietary fiber	2174:2186	The degree of this increase may differ between sows and GF pigs and seems to depend on properties of dietary fiber.
28464099	6	63	theme	92.5	1189:1192	arg1	%					1202:1202	92.5 vs. 88.4%	1189:1202	%	1202:1202	While digestibility of OM and COH was similar in sows and GF pigs that were fed the STA diet and the CEL diet, on the INU diet, sows had, compared with GF pigs, a greater digestibility of OM (92.2 vs. 87.2%) and COH (92.5 vs. 88.4%), respectively.
28464099	6	63	theme	92.5	1189:1192	arg1	COH					1184:1186	COH	1184:1186	COH (92.5 vs. 88.4%)	1184:1203	While digestibility of OM and COH was similar in sows and GF pigs that were fed the STA diet and the CEL diet, on the INU diet, sows had, compared with GF pigs, a greater digestibility of OM (92.2 vs. 87.2%) and COH (92.5 vs. 88.4%), respectively.
28464099	9	64	from	similar	1527:1533	arg1	those					1589:1593	those	1589:1593	those	1589:1593	When expressed in mg/(kg BW·d), however, P excretion was similar in GF pigs and sows that were fed the STA diet and in those that were fed the INU diet, whereas it was greater in sows than in GF pigs that were fed the CEL diet (11.6 vs. 7.3 mg/(kg BW·d)).
28464099	9	64	from	similar	1527:1533	arg1	pigs					1541:1544	GF pigs	1538:1544	GF pigs	1538:1544	When expressed in mg/(kg BW·d), however, P excretion was similar in GF pigs and sows that were fed the STA diet and in those that were fed the INU diet, whereas it was greater in sows than in GF pigs that were fed the CEL diet (11.6 vs. 7.3 mg/(kg BW·d)).
28464099	4	65	from	CP	669:670	arg1	DMI					746:748	mg/kg DMI	740:748	mg/kg DMI	740:748	Digestibility of DM, OM, CP, crude fat, and carbohydrates (COH), and fecal P excretion (in g/d, mg/kg DMI, and g/(kg BW·d)) were determined using TiO as indigestible marker.
28464099	4	65	from	CP	669:670	arg1	g/					755:756	g/	755:756	g/(kg BW·d)	755:765	Digestibility of DM, OM, CP, crude fat, and carbohydrates (COH), and fecal P excretion (in g/d, mg/kg DMI, and g/(kg BW·d)) were determined using TiO as indigestible marker.
28464099	4	65	from	CP	669:670	arg1	BW·d					761:764	kg BW·d	758:764	kg BW·d	758:764	Digestibility of DM, OM, CP, crude fat, and carbohydrates (COH), and fecal P excretion (in g/d, mg/kg DMI, and g/(kg BW·d)) were determined using TiO as indigestible marker.
28464099	4	65	from	CP	669:670	arg1	g/d					735:737	g/d	735:737	g/d	735:737	Digestibility of DM, OM, CP, crude fat, and carbohydrates (COH), and fecal P excretion (in g/d, mg/kg DMI, and g/(kg BW·d)) were determined using TiO as indigestible marker.
28464099	3	66	dep	type	490:493	arg1	sows					507:510	sows	507:510	sows	507:510	Treatments were animal type (GF pigs or sows), diet composition (a semipurified starch (STA), inulin (INU), or lignocellulose (CEL) based low-P diet), and FL (2.0 or 3.0 kg/d).
28464099	3	66	dep	type	490:493	arg1	pigs					499:502	GF pigs	496:502	GF pigs	496:502	Treatments were animal type (GF pigs or sows), diet composition (a semipurified starch (STA), inulin (INU), or lignocellulose (CEL) based low-P diet), and FL (2.0 or 3.0 kg/d).
28464099	8	67	theme	STA	1353:1355	arg1	diet					1357:1360	the STA diet	1349:1360	the STA diet (498 versus 236 mg/kg DMI)	1349:1387	When expressed in mg/kg DMI, P excretion was higher in sows than in GF pigs on the STA diet (498 versus 236 mg/kg DMI), the INU diet (526 vs. 316 mg/kg DMI), and the CEL diet (928 vs. 342 mg/kg DMI).
28464099	8	67	theme	STA	1353:1355	arg1	DMI					1384:1386	498 versus 236 mg/kg DMI	1363:1386	498 versus 236 mg/kg DMI	1363:1386	When expressed in mg/kg DMI, P excretion was higher in sows than in GF pigs on the STA diet (498 versus 236 mg/kg DMI), the INU diet (526 vs. 316 mg/kg DMI), and the CEL diet (928 vs. 342 mg/kg DMI).
28464099	4	68	from	OM	665:666	arg1	DMI					746:748	mg/kg DMI	740:748	mg/kg DMI	740:748	Digestibility of DM, OM, CP, crude fat, and carbohydrates (COH), and fecal P excretion (in g/d, mg/kg DMI, and g/(kg BW·d)) were determined using TiO as indigestible marker.
28464099	4	68	from	OM	665:666	arg1	g/					755:756	g/	755:756	g/(kg BW·d)	755:765	Digestibility of DM, OM, CP, crude fat, and carbohydrates (COH), and fecal P excretion (in g/d, mg/kg DMI, and g/(kg BW·d)) were determined using TiO as indigestible marker.
28464099	4	68	from	OM	665:666	arg1	BW·d					761:764	kg BW·d	758:764	kg BW·d	758:764	Digestibility of DM, OM, CP, crude fat, and carbohydrates (COH), and fecal P excretion (in g/d, mg/kg DMI, and g/(kg BW·d)) were determined using TiO as indigestible marker.
28464099	4	68	from	OM	665:666	arg1	g/d					735:737	g/d	735:737	g/d	735:737	Digestibility of DM, OM, CP, crude fat, and carbohydrates (COH), and fecal P excretion (in g/d, mg/kg DMI, and g/(kg BW·d)) were determined using TiO as indigestible marker.
28464099	9	69	theme	GF	1662:1663	arg1	pigs					1665:1668	GF pigs	1662:1668	GF pigs that were fed the CEL diet (11.6 vs. 7.3 mg/(kg BW·d))	1662:1723	When expressed in mg/(kg BW·d), however, P excretion was similar in GF pigs and sows that were fed the STA diet and in those that were fed the INU diet, whereas it was greater in sows than in GF pigs that were fed the CEL diet (11.6 vs. 7.3 mg/(kg BW·d)).
28464099	6	70	theme	GF	1124:1125	arg1	pigs					1127:1130	GF pigs	1124:1130	GF pigs	1124:1130	While digestibility of OM and COH was similar in sows and GF pigs that were fed the STA diet and the CEL diet, on the INU diet, sows had, compared with GF pigs, a greater digestibility of OM (92.2 vs. 87.2%) and COH (92.5 vs. 88.4%), respectively.
28464099	2	71	theme	×	440:440	arg1	arrangement					454:464	a 2 × 3 × 2 factorial arrangement	432:464	a 2 × 3 × 2 factorial arrangement	432:464	After an adaptation period, 48 GF pigs (initial BW 90.5 kg) and 48 just-weaned sows (initial BW 195 kg), both individually housed, were allotted to 12 dietary treatments in a 2 × 3 × 2 factorial arrangement.
28464099	11	72	theme	EPL	1848:1850	arg1	Application					1833:1843	Application	1833:1843	Application of EPL (mg/kg DMI) determined in GF pigs	1833:1884	Application of EPL (mg/kg DMI) determined in GF pigs may underestimate EPL and therefore P requirements in gestating sows.
28464099	4	73	theme	DM	661:662	arg1	CP					669:670	CP	669:670	CP	669:670	Digestibility of DM, OM, CP, crude fat, and carbohydrates (COH), and fecal P excretion (in g/d, mg/kg DMI, and g/(kg BW·d)) were determined using TiO as indigestible marker.
28464099	4	73	theme	DM	661:662	arg1	carbohydrates					688:700	carbohydrates	688:700	carbohydrates (COH)	688:706	Digestibility of DM, OM, CP, crude fat, and carbohydrates (COH), and fecal P excretion (in g/d, mg/kg DMI, and g/(kg BW·d)) were determined using TiO as indigestible marker.
28464099	4	73	theme	DM	661:662	arg1	Digestibility					644:656	Digestibility	644:656	Digestibility of DM	644:662	Digestibility of DM, OM, CP, crude fat, and carbohydrates (COH), and fecal P excretion (in g/d, mg/kg DMI, and g/(kg BW·d)) were determined using TiO as indigestible marker.
28464099	4	73	theme	DM	661:662	arg1	COH					703:705	COH	703:705	COH	703:705	Digestibility of DM, OM, CP, crude fat, and carbohydrates (COH), and fecal P excretion (in g/d, mg/kg DMI, and g/(kg BW·d)) were determined using TiO as indigestible marker.
28464099	4	73	theme	DM	661:662	arg1	excretion					721:729	fecal P excretion	713:729	fecal P excretion (in g/d, mg/kg DMI, and g/(kg BW·d))	713:766	Digestibility of DM, OM, CP, crude fat, and carbohydrates (COH), and fecal P excretion (in g/d, mg/kg DMI, and g/(kg BW·d)) were determined using TiO as indigestible marker.
28464099	4	73	theme	DM	661:662	arg1	fat					679:681	crude fat	673:681	crude fat	673:681	Digestibility of DM, OM, CP, crude fat, and carbohydrates (COH), and fecal P excretion (in g/d, mg/kg DMI, and g/(kg BW·d)) were determined using TiO as indigestible marker.
28464099	4	73	theme	DM	661:662	arg1	OM					665:666	OM	665:666	OM	665:666	Digestibility of DM, OM, CP, crude fat, and carbohydrates (COH), and fecal P excretion (in g/d, mg/kg DMI, and g/(kg BW·d)) were determined using TiO as indigestible marker.
28464099	5	74	theme	pigs	912:915	arg1	types					903:907	both types	898:907	both types of pigs fed the STA diet	898:932	Digestibility of OM and COH differed among diets ( < 0.001) and was greatest in both types of pigs fed the STA diet and lowest in those fed the CEL diet.
28464099	9	75	theme	P	1511:1511	arg1	excretion					1513:1521	P excretion	1511:1521	P excretion	1511:1521	When expressed in mg/(kg BW·d), however, P excretion was similar in GF pigs and sows that were fed the STA diet and in those that were fed the INU diet, whereas it was greater in sows than in GF pigs that were fed the CEL diet (11.6 vs. 7.3 mg/(kg BW·d)).
28464099	6	76	theme	OM	995:996	arg1	digestibility					978:990	digestibility	978:990	digestibility of OM and COH	978:1004	While digestibility of OM and COH was similar in sows and GF pigs that were fed the STA diet and the CEL diet, on the INU diet, sows had, compared with GF pigs, a greater digestibility of OM (92.2 vs. 87.2%) and COH (92.5 vs. 88.4%), respectively.
28464099	4	77	from	Digestibility	644:656	arg1	DMI					746:748	mg/kg DMI	740:748	mg/kg DMI	740:748	Digestibility of DM, OM, CP, crude fat, and carbohydrates (COH), and fecal P excretion (in g/d, mg/kg DMI, and g/(kg BW·d)) were determined using TiO as indigestible marker.
28464099	4	77	from	Digestibility	644:656	arg1	g/					755:756	g/	755:756	g/(kg BW·d)	755:765	Digestibility of DM, OM, CP, crude fat, and carbohydrates (COH), and fecal P excretion (in g/d, mg/kg DMI, and g/(kg BW·d)) were determined using TiO as indigestible marker.
28464099	4	77	from	Digestibility	644:656	arg1	BW·d					761:764	kg BW·d	758:764	kg BW·d	758:764	Digestibility of DM, OM, CP, crude fat, and carbohydrates (COH), and fecal P excretion (in g/d, mg/kg DMI, and g/(kg BW·d)) were determined using TiO as indigestible marker.
28464099	4	77	from	Digestibility	644:656	arg1	g/d					735:737	g/d	735:737	g/d	735:737	Digestibility of DM, OM, CP, crude fat, and carbohydrates (COH), and fecal P excretion (in g/d, mg/kg DMI, and g/(kg BW·d)) were determined using TiO as indigestible marker.
28464099	1	78	theme	composition	136:146	arg1	effects					120:126	the effects	116:126	the effects of diet composition, feeding level (FL), and BW on endogenous phosphorous losses (EPL) using growing-finishing (GF) pigs and sows	116:256	An experiment was conducted to determine the effects of diet composition, feeding level (FL), and BW on endogenous phosphorous losses (EPL) using growing-finishing (GF) pigs and sows.
28464099	8	79	theme	mg/kg	1378:1382	arg1	diet					1357:1360	the STA diet	1349:1360	the STA diet (498 versus 236 mg/kg DMI)	1349:1387	When expressed in mg/kg DMI, P excretion was higher in sows than in GF pigs on the STA diet (498 versus 236 mg/kg DMI), the INU diet (526 vs. 316 mg/kg DMI), and the CEL diet (928 vs. 342 mg/kg DMI).
28464099	8	79	theme	mg/kg	1378:1382	arg1	DMI					1384:1386	498 versus 236 mg/kg DMI	1363:1386	498 versus 236 mg/kg DMI	1363:1386	When expressed in mg/kg DMI, P excretion was higher in sows than in GF pigs on the STA diet (498 versus 236 mg/kg DMI), the INU diet (526 vs. 316 mg/kg DMI), and the CEL diet (928 vs. 342 mg/kg DMI).
28464099	9	80	theme	CEL	1688:1690	arg1	diet					1692:1695	the CEL diet	1684:1695	the CEL diet (11.6 vs. 7.3 mg/(kg BW·d))	1684:1723	When expressed in mg/(kg BW·d), however, P excretion was similar in GF pigs and sows that were fed the STA diet and in those that were fed the INU diet, whereas it was greater in sows than in GF pigs that were fed the CEL diet (11.6 vs. 7.3 mg/(kg BW·d)).
28464099	1	81	theme	feeding	149:155	arg1	FL					164:165	FL	164:165	FL	164:165	An experiment was conducted to determine the effects of diet composition, feeding level (FL), and BW on endogenous phosphorous losses (EPL) using growing-finishing (GF) pigs and sows.
28464099	1	81	theme	feeding	149:155	arg1	level					157:161	feeding level	149:161	feeding level (FL)	149:166	An experiment was conducted to determine the effects of diet composition, feeding level (FL), and BW on endogenous phosphorous losses (EPL) using growing-finishing (GF) pigs and sows.
28464099	11	82	theme	mg/kg	1853:1857	arg1	EPL					1848:1850	EPL	1848:1850	EPL (mg/kg DMI) determined in GF pigs	1848:1884	Application of EPL (mg/kg DMI) determined in GF pigs may underestimate EPL and therefore P requirements in gestating sows.
28464099	11	82	theme	mg/kg	1853:1857	arg1	DMI					1859:1861	mg/kg DMI	1853:1861	mg/kg DMI	1853:1861	Application of EPL (mg/kg DMI) determined in GF pigs may underestimate EPL and therefore P requirements in gestating sows.
28464099	4	83	theme	crude	673:677	arg1	fat					679:681	crude fat	673:681	crude fat	673:681	Digestibility of DM, OM, CP, crude fat, and carbohydrates (COH), and fecal P excretion (in g/d, mg/kg DMI, and g/(kg BW·d)) were determined using TiO as indigestible marker.
28464099	12	84	theme	polysaccharides	2050:2064	arg1	content					2021:2027	an increasing content	2007:2027	an increasing content of dietary nonstarch polysaccharides (NSP)	2007:2070	Moreover, EPL is diet dependent and increases with an increasing content of dietary nonstarch polysaccharides (NSP).
28464099	6	85	theme	88.4	1198:1201	arg1	%					1202:1202	92.5 vs. 88.4%	1189:1202	%	1202:1202	While digestibility of OM and COH was similar in sows and GF pigs that were fed the STA diet and the CEL diet, on the INU diet, sows had, compared with GF pigs, a greater digestibility of OM (92.2 vs. 87.2%) and COH (92.5 vs. 88.4%), respectively.
28464099	6	85	theme	88.4	1198:1201	arg1	COH					1184:1186	COH	1184:1186	COH (92.5 vs. 88.4%)	1184:1203	While digestibility of OM and COH was similar in sows and GF pigs that were fed the STA diet and the CEL diet, on the INU diet, sows had, compared with GF pigs, a greater digestibility of OM (92.2 vs. 87.2%) and COH (92.5 vs. 88.4%), respectively.
28464099	2	86	from	treatments	418:427	arg1	arrangement					454:464	a 2 × 3 × 2 factorial arrangement	432:464	a 2 × 3 × 2 factorial arrangement	432:464	After an adaptation period, 48 GF pigs (initial BW 90.5 kg) and 48 just-weaned sows (initial BW 195 kg), both individually housed, were allotted to 12 dietary treatments in a 2 × 3 × 2 factorial arrangement.
28464099	0	87	theme	phosphorus	11:20	arg1	losses					22:27	Endogenous phosphorus losses	0:27	Endogenous phosphorus losses in growing-finishing pigs and gestating sows	0:72	Endogenous phosphorus losses in growing-finishing pigs and gestating sows.
28464099	6	88	theme	greater	1135:1141	arg1	%					1177:1177	92.2 vs. 87.2%	1164:1177	92.2 vs. 87.2%	1164:1177	While digestibility of OM and COH was similar in sows and GF pigs that were fed the STA diet and the CEL diet, on the INU diet, sows had, compared with GF pigs, a greater digestibility of OM (92.2 vs. 87.2%) and COH (92.5 vs. 88.4%), respectively.
28464099	6	88	theme	greater	1135:1141	arg1	digestibility					1143:1155	a greater digestibility	1133:1155	a greater digestibility of OM (92.2 vs. 87.2%)	1133:1178	While digestibility of OM and COH was similar in sows and GF pigs that were fed the STA diet and the CEL diet, on the INU diet, sows had, compared with GF pigs, a greater digestibility of OM (92.2 vs. 87.2%) and COH (92.5 vs. 88.4%), respectively.
28464099	2	89	theme	BW	352:353	arg1	kg					359:360	initial BW 195 kg	344:360	initial BW 195 kg	344:360	After an adaptation period, 48 GF pigs (initial BW 90.5 kg) and 48 just-weaned sows (initial BW 195 kg), both individually housed, were allotted to 12 dietary treatments in a 2 × 3 × 2 factorial arrangement.
28464099	2	89	theme	BW	352:353	arg1	sows					338:341	48 just-weaned sows	323:341	48 just-weaned sows (initial BW 195 kg)	323:361	After an adaptation period, 48 GF pigs (initial BW 90.5 kg) and 48 just-weaned sows (initial BW 195 kg), both individually housed, were allotted to 12 dietary treatments in a 2 × 3 × 2 factorial arrangement.
28464099	10	90	theme	mg/kg	1771:1775	arg1	EPL					1766:1768	EPL	1766:1768	EPL (mg/kg DMI) in pigs	1766:1788	The results of this study indicate that EPL (mg/kg DMI) in pigs substantially increase with increasing BW.
28464099	10	90	theme	mg/kg	1771:1775	arg1	DMI					1777:1779	mg/kg DMI	1771:1779	mg/kg DMI	1771:1779	The results of this study indicate that EPL (mg/kg DMI) in pigs substantially increase with increasing BW.
28464099	2	91	theme	BW	307:308	arg1	kg					315:316	initial BW 90.5 kg	299:316	initial BW 90.5 kg	299:316	After an adaptation period, 48 GF pigs (initial BW 90.5 kg) and 48 just-weaned sows (initial BW 195 kg), both individually housed, were allotted to 12 dietary treatments in a 2 × 3 × 2 factorial arrangement.
28464099	2	91	theme	BW	307:308	arg1	pigs					293:296	48 GF pigs	287:296	48 GF pigs (initial BW 90.5 kg)	287:317	After an adaptation period, 48 GF pigs (initial BW 90.5 kg) and 48 just-weaned sows (initial BW 195 kg), both individually housed, were allotted to 12 dietary treatments in a 2 × 3 × 2 factorial arrangement.
28464099	5	92	theme	OM	835:836	arg1	Digestibility					818:830	Digestibility	818:830	Digestibility of OM and COH	818:844	Digestibility of OM and COH differed among diets ( < 0.001) and was greatest in both types of pigs fed the STA diet and lowest in those fed the CEL diet.
28464099	1	93	from	effects	120:126	arg1	EPL					210:212	EPL	210:212	EPL	210:212	An experiment was conducted to determine the effects of diet composition, feeding level (FL), and BW on endogenous phosphorous losses (EPL) using growing-finishing (GF) pigs and sows.
28464099	1	93	from	effects	120:126	arg1	losses					202:207	endogenous phosphorous losses	179:207	endogenous phosphorous losses (EPL) using growing-finishing (GF) pigs and sows	179:256	An experiment was conducted to determine the effects of diet composition, feeding level (FL), and BW on endogenous phosphorous losses (EPL) using growing-finishing (GF) pigs and sows.
28464099	2	94	theme	just-weaned	326:336	arg1	kg					359:360	initial BW 195 kg	344:360	initial BW 195 kg	344:360	After an adaptation period, 48 GF pigs (initial BW 90.5 kg) and 48 just-weaned sows (initial BW 195 kg), both individually housed, were allotted to 12 dietary treatments in a 2 × 3 × 2 factorial arrangement.
28464099	2	94	theme	just-weaned	326:336	arg1	sows					338:341	48 just-weaned sows	323:341	48 just-weaned sows (initial BW 195 kg)	323:361	After an adaptation period, 48 GF pigs (initial BW 90.5 kg) and 48 just-weaned sows (initial BW 195 kg), both individually housed, were allotted to 12 dietary treatments in a 2 × 3 × 2 factorial arrangement.
28464099	4	95	from	excretion	721:729	arg1	DMI					746:748	mg/kg DMI	740:748	mg/kg DMI	740:748	Digestibility of DM, OM, CP, crude fat, and carbohydrates (COH), and fecal P excretion (in g/d, mg/kg DMI, and g/(kg BW·d)) were determined using TiO as indigestible marker.
28464099	4	95	from	excretion	721:729	arg1	g/					755:756	g/	755:756	g/(kg BW·d)	755:765	Digestibility of DM, OM, CP, crude fat, and carbohydrates (COH), and fecal P excretion (in g/d, mg/kg DMI, and g/(kg BW·d)) were determined using TiO as indigestible marker.
28464099	4	95	from	excretion	721:729	arg1	BW·d					761:764	kg BW·d	758:764	kg BW·d	758:764	Digestibility of DM, OM, CP, crude fat, and carbohydrates (COH), and fecal P excretion (in g/d, mg/kg DMI, and g/(kg BW·d)) were determined using TiO as indigestible marker.
28464099	4	95	from	excretion	721:729	arg1	g/d					735:737	g/d	735:737	g/d	735:737	Digestibility of DM, OM, CP, crude fat, and carbohydrates (COH), and fecal P excretion (in g/d, mg/kg DMI, and g/(kg BW·d)) were determined using TiO as indigestible marker.
28464099	3	96	theme	GF	496:497	arg1	pigs					499:502	GF pigs	496:502	GF pigs	496:502	Treatments were animal type (GF pigs or sows), diet composition (a semipurified starch (STA), inulin (INU), or lignocellulose (CEL) based low-P diet), and FL (2.0 or 3.0 kg/d).
28464099	0	97	theme	gestating	59:67	arg1	sows					69:72	gestating sows	59:72	gestating sows	59:72	Endogenous phosphorus losses in growing-finishing pigs and gestating sows.
28464099	13	98	theme	increase	2092:2099	arg1	degree					2077:2082	The degree	2073:2082	The degree of this increase	2073:2099	The degree of this increase may differ between sows and GF pigs and seems to depend on properties of dietary fiber.
28464099	4	99	theme	fecal	713:717	arg1	excretion					721:729	fecal P excretion	713:729	fecal P excretion (in g/d, mg/kg DMI, and g/(kg BW·d))	713:766	Digestibility of DM, OM, CP, crude fat, and carbohydrates (COH), and fecal P excretion (in g/d, mg/kg DMI, and g/(kg BW·d)) were determined using TiO as indigestible marker.
28464099	3	100	theme	diet	514:517	arg1	composition					519:529	diet composition	514:529	diet composition (a semipurified starch (STA), inulin (INU), or lignocellulose (CEL) based low-P diet)	514:615	Treatments were animal type (GF pigs or sows), diet composition (a semipurified starch (STA), inulin (INU), or lignocellulose (CEL) based low-P diet), and FL (2.0 or 3.0 kg/d).
28464099	11	101	theme	P	1922:1922	arg1	requirements					1924:1935	P requirements	1922:1935	P requirements	1922:1935	Application of EPL (mg/kg DMI) determined in GF pigs may underestimate EPL and therefore P requirements in gestating sows.
28464099	12	102	theme	dietary	2032:2038	arg1	NSP					2067:2069	NSP	2067:2069	NSP	2067:2069	Moreover, EPL is diet dependent and increases with an increasing content of dietary nonstarch polysaccharides (NSP).
28464099	12	102	theme	dietary	2032:2038	arg1	polysaccharides					2050:2064	dietary nonstarch polysaccharides	2032:2064	dietary nonstarch polysaccharides (NSP)	2032:2070	Moreover, EPL is diet dependent and increases with an increasing content of dietary nonstarch polysaccharides (NSP).
28464099	2	103	theme	adaptation	268:277	arg1	period					279:284	an adaptation period	265:284	an adaptation period	265:284	After an adaptation period, 48 GF pigs (initial BW 90.5 kg) and 48 just-weaned sows (initial BW 195 kg), both individually housed, were allotted to 12 dietary treatments in a 2 × 3 × 2 factorial arrangement.
28464099	6	104	theme	STA	1056:1058	arg1	diet					1060:1063	the STA diet	1052:1063	the STA diet	1052:1063	While digestibility of OM and COH was similar in sows and GF pigs that were fed the STA diet and the CEL diet, on the INU diet, sows had, compared with GF pigs, a greater digestibility of OM (92.2 vs. 87.2%) and COH (92.5 vs. 88.4%), respectively.
28464099	3	105	theme	semipurified	534:545	arg1	STA					555:557	STA	555:557	STA	555:557	Treatments were animal type (GF pigs or sows), diet composition (a semipurified starch (STA), inulin (INU), or lignocellulose (CEL) based low-P diet), and FL (2.0 or 3.0 kg/d).
28464099	3	105	theme	semipurified	534:545	arg1	starch					547:552	a semipurified starch	532:552	a semipurified starch (STA)	532:558	Treatments were animal type (GF pigs or sows), diet composition (a semipurified starch (STA), inulin (INU), or lignocellulose (CEL) based low-P diet), and FL (2.0 or 3.0 kg/d).
28464099	6	106	theme	GF	1030:1031	arg1	pigs					1033:1036	GF pigs	1030:1036	GF pigs	1030:1036	While digestibility of OM and COH was similar in sows and GF pigs that were fed the STA diet and the CEL diet, on the INU diet, sows had, compared with GF pigs, a greater digestibility of OM (92.2 vs. 87.2%) and COH (92.5 vs. 88.4%), respectively.
28464099	4	107	from	carbohydrates	688:700	arg1	DMI					746:748	mg/kg DMI	740:748	mg/kg DMI	740:748	Digestibility of DM, OM, CP, crude fat, and carbohydrates (COH), and fecal P excretion (in g/d, mg/kg DMI, and g/(kg BW·d)) were determined using TiO as indigestible marker.
28464099	4	107	from	carbohydrates	688:700	arg1	g/					755:756	g/	755:756	g/(kg BW·d)	755:765	Digestibility of DM, OM, CP, crude fat, and carbohydrates (COH), and fecal P excretion (in g/d, mg/kg DMI, and g/(kg BW·d)) were determined using TiO as indigestible marker.
28464099	4	107	from	carbohydrates	688:700	arg1	BW·d					761:764	kg BW·d	758:764	kg BW·d	758:764	Digestibility of DM, OM, CP, crude fat, and carbohydrates (COH), and fecal P excretion (in g/d, mg/kg DMI, and g/(kg BW·d)) were determined using TiO as indigestible marker.
28464099	4	107	from	carbohydrates	688:700	arg1	g/d					735:737	g/d	735:737	g/d	735:737	Digestibility of DM, OM, CP, crude fat, and carbohydrates (COH), and fecal P excretion (in g/d, mg/kg DMI, and g/(kg BW·d)) were determined using TiO as indigestible marker.
25434103	0	0	theme	recovery--a	79:89	arg1	approach					103:110	sugar recovery--a statistical approach	73:110	sugar recovery--a statistical approach	73:110	Dilute acid pretreatment and enzymatic hydrolysis of sorghum biomass for sugar recovery--a statistical approach.
25434103	5	1	theme	sugars	811:816	arg1	production					778:787	production	778:787	production of the total reducing sugars released during the process	778:844	The efficiency of the process was evaluated on the basis of production of the total reducing sugars released during the process.
25434103	8	2	theme	cellulose	1082:1090	arg1	content					1092:1098	The cellulose content	1078:1098	The cellulose content in the solid portion obtained after pretreatment using optimised conditions	1078:1174	The cellulose content in the solid portion obtained after pretreatment using optimised conditions was found to be increased by 43.37% with lesser production of inhibitors in acid pretreated liquor.
25434103	1	3	theme	lignocellulosic	157:171	arg1	biomass					173:179	the commercially feasible lignocellulosic biomass	131:179	the commercially feasible lignocellulosic biomass	131:179	Sorghum is one of the commercially feasible lignocellulosic biomass and has a great potential of being sustainable feedstock for renewable energy.
25434103	0	4	theme	sugar	73:77	arg1	approach					103:110	sugar recovery--a statistical approach	73:110	sugar recovery--a statistical approach	73:110	Dilute acid pretreatment and enzymatic hydrolysis of sorghum biomass for sugar recovery--a statistical approach.
25434103	6	5	theme	native	883:888	arg1	biomass					912:918	native as well as pretreated biomass	883:918	native as well as pretreated biomass	883:918	Compositional analysis was done for native as well as pretreated biomass and compared.
25434103	7	6	theme	reducing	1010:1017	arg1	sugars					1019:1024	reducing sugars	1010:1024	reducing sugars	1010:1024	The biomass pretreated with the optimized conditions could yield 0.408 g of reducing sugars /g of pretreated biomass upon enzymatic hydrolysis.
25434103	3	7	theme	maximum	522:528	arg1	sugars					542:547	maximum fermentable sugars	522:547	maximum fermentable sugars	522:547	In the present study, sorghum biomass was evaluated for deriving maximum fermentable sugars by optimizing various pretreatment parameters using statistical optimization methods.
25434103	7	8	theme	sugars	1019:1024	arg1	g					1005:1005	0.408 g	999:1005	0.408 g of reducing sugars /g of pretreated biomass	999:1049	The biomass pretreated with the optimized conditions could yield 0.408 g of reducing sugars /g of pretreated biomass upon enzymatic hydrolysis.
25434103	3	9	theme	various	563:569	arg1	parameters					584:593	various pretreatment parameters	563:593	various pretreatment parameters using statistical optimization methods	563:632	In the present study, sorghum biomass was evaluated for deriving maximum fermentable sugars by optimizing various pretreatment parameters using statistical optimization methods.
25434103	0	10	theme	statistical	91:101	arg1	approach					103:110	sugar recovery--a statistical approach	73:110	sugar recovery--a statistical approach	73:110	Dilute acid pretreatment and enzymatic hydrolysis of sorghum biomass for sugar recovery--a statistical approach.
25434103	3	11	theme	pretreatment	571:582	arg1	parameters					584:593	various pretreatment parameters	563:593	various pretreatment parameters using statistical optimization methods	563:632	In the present study, sorghum biomass was evaluated for deriving maximum fermentable sugars by optimizing various pretreatment parameters using statistical optimization methods.
25434103	4	12	theme	Pretreatment	635:646	arg1	studies					648:654	Pretreatment studies	635:654	Pretreatment studies	635:654	Pretreatment studies were done with H2SO4, followed by enzymatic saccharification.
25434103	8	13	theme	solid	1107:1111	arg1	portion					1113:1119	the solid portion	1103:1119	the solid portion obtained after pretreatment using optimised conditions	1103:1174	The cellulose content in the solid portion obtained after pretreatment using optimised conditions was found to be increased by 43.37% with lesser production of inhibitors in acid pretreated liquor.
25434103	6	14	theme	Compositional	847:859	arg1	analysis					861:868	Compositional analysis	847:868	Compositional analysis	847:868	Compositional analysis was done for native as well as pretreated biomass and compared.
25434103	8	15	theme	lesser	1217:1222	arg1	production					1224:1233	lesser production	1217:1233	lesser production of inhibitors in acid pretreated liquor	1217:1273	The cellulose content in the solid portion obtained after pretreatment using optimised conditions was found to be increased by 43.37% with lesser production of inhibitors in acid pretreated liquor.
25434103	2	16	theme	lignocellulosic	272:286	arg1	biomass					288:294	any lignocellulosic biomass	268:294	any lignocellulosic biomass	268:294	As with any lignocellulosic biomass, sorghum also requires pretreatment which increases its susceptibility to hydrolysis by enzymes for generating sugars which can be further fermented to alcohol.
25434103	0	17	theme	acid	7:10	arg1	pretreatment					12:23	Dilute acid pretreatment	0:23	Dilute acid pretreatment	0:23	Dilute acid pretreatment and enzymatic hydrolysis of sorghum biomass for sugar recovery--a statistical approach.
25434103	3	18	theme	statistical	601:611	arg1	methods					626:632	statistical optimization methods	601:632	statistical optimization methods	601:632	In the present study, sorghum biomass was evaluated for deriving maximum fermentable sugars by optimizing various pretreatment parameters using statistical optimization methods.
25434103	0	19	theme	Dilute	0:5	arg1	pretreatment					12:23	Dilute acid pretreatment	0:23	Dilute acid pretreatment	0:23	Dilute acid pretreatment and enzymatic hydrolysis of sorghum biomass for sugar recovery--a statistical approach.
25434103	3	20	theme	optimization	613:624	arg1	methods					626:632	statistical optimization methods	601:632	statistical optimization methods	601:632	In the present study, sorghum biomass was evaluated for deriving maximum fermentable sugars by optimizing various pretreatment parameters using statistical optimization methods.
25434103	1	21	theme	great	191:195	arg1	potential					197:205	a great potential	189:205	a great potential of being sustainable feedstock for renewable energy	189:257	Sorghum is one of the commercially feasible lignocellulosic biomass and has a great potential of being sustainable feedstock for renewable energy.
25434103	8	22	theme	acid	1252:1255	arg1	liquor					1268:1273	acid pretreated liquor	1252:1273	acid pretreated liquor	1252:1273	The cellulose content in the solid portion obtained after pretreatment using optimised conditions was found to be increased by 43.37% with lesser production of inhibitors in acid pretreated liquor.
25434103	8	23	from	content	1092:1098	arg1	portion					1113:1119	the solid portion	1103:1119	the solid portion obtained after pretreatment using optimised conditions	1103:1174	The cellulose content in the solid portion obtained after pretreatment using optimised conditions was found to be increased by 43.37% with lesser production of inhibitors in acid pretreated liquor.
25434103	1	24	theme	biomass	173:179	arg1	one					124:126	one	124:126	one	124:126	Sorghum is one of the commercially feasible lignocellulosic biomass and has a great potential of being sustainable feedstock for renewable energy.
25434103	1	24	theme	biomass	173:179	arg1	biomass					173:179	the commercially feasible lignocellulosic biomass	131:179	the commercially feasible lignocellulosic biomass	131:179	Sorghum is one of the commercially feasible lignocellulosic biomass and has a great potential of being sustainable feedstock for renewable energy.
25434103	3	25	theme	sorghum	479:485	arg1	biomass					487:493	sorghum biomass	479:493	sorghum biomass	479:493	In the present study, sorghum biomass was evaluated for deriving maximum fermentable sugars by optimizing various pretreatment parameters using statistical optimization methods.
25434103	8	26	theme	optimised	1155:1163	arg1	conditions					1165:1174	optimised conditions	1155:1174	optimised conditions	1155:1174	The cellulose content in the solid portion obtained after pretreatment using optimised conditions was found to be increased by 43.37% with lesser production of inhibitors in acid pretreated liquor.
25434103	4	27	theme	enzymatic	690:698	arg1	saccharification					700:715	enzymatic saccharification	690:715	enzymatic saccharification	690:715	Pretreatment studies were done with H2SO4, followed by enzymatic saccharification.
25434103	3	28	theme	present	464:470	arg1	study					472:476	the present study	460:476	the present study	460:476	In the present study, sorghum biomass was evaluated for deriving maximum fermentable sugars by optimizing various pretreatment parameters using statistical optimization methods.
25434103	0	29	theme	enzymatic	29:37	arg1	hydrolysis					39:48	enzymatic hydrolysis	29:48	enzymatic hydrolysis	29:48	Dilute acid pretreatment and enzymatic hydrolysis of sorghum biomass for sugar recovery--a statistical approach.
25434103	8	30	theme	pretreated	1257:1266	arg1	liquor					1268:1273	acid pretreated liquor	1252:1273	acid pretreated liquor	1252:1273	The cellulose content in the solid portion obtained after pretreatment using optimised conditions was found to be increased by 43.37% with lesser production of inhibitors in acid pretreated liquor.
25434103	1	31	contain	has	185:187	arg2	potential					197:205	a great potential	189:205	a great potential of being sustainable feedstock for renewable energy	189:257	Sorghum is one of the commercially feasible lignocellulosic biomass and has a great potential of being sustainable feedstock for renewable energy.
25434103	1	31	contain	has	185:187	arg1	Sorghum					113:119	Sorghum	113:119	Sorghum	113:119	Sorghum is one of the commercially feasible lignocellulosic biomass and has a great potential of being sustainable feedstock for renewable energy.
25434103	1	32	theme	sustainable	216:226	arg1	feedstock					228:236	sustainable feedstock	216:236	sustainable feedstock for renewable energy	216:257	Sorghum is one of the commercially feasible lignocellulosic biomass and has a great potential of being sustainable feedstock for renewable energy.
25434103	0	33	theme	sorghum	53:59	arg1	biomass					61:67	sorghum biomass	53:67	sorghum biomass	53:67	Dilute acid pretreatment and enzymatic hydrolysis of sorghum biomass for sugar recovery--a statistical approach.
25434103	5	34	theme	reducing	802:809	arg1	sugars					811:816	the total reducing sugars	792:816	the total reducing sugars released during the process	792:844	The efficiency of the process was evaluated on the basis of production of the total reducing sugars released during the process.
25434103	8	35	from	production	1224:1233	arg1	liquor					1268:1273	acid pretreated liquor	1252:1273	acid pretreated liquor	1252:1273	The cellulose content in the solid portion obtained after pretreatment using optimised conditions was found to be increased by 43.37% with lesser production of inhibitors in acid pretreated liquor.
25434103	6	36	theme	pretreated	901:910	arg1	biomass					912:918	native as well as pretreated biomass	883:918	native as well as pretreated biomass	883:918	Compositional analysis was done for native as well as pretreated biomass and compared.
25434103	7	37	dep	g	1005:1005	arg1	/g					1026:1027	/g	1026:1027	0.408 g of reducing sugars /g of pretreated biomass	999:1049	The biomass pretreated with the optimized conditions could yield 0.408 g of reducing sugars /g of pretreated biomass upon enzymatic hydrolysis.
25434103	8	38	theme	inhibitors	1238:1247	arg1	production					1224:1233	lesser production	1217:1233	lesser production of inhibitors in acid pretreated liquor	1217:1273	The cellulose content in the solid portion obtained after pretreatment using optimised conditions was found to be increased by 43.37% with lesser production of inhibitors in acid pretreated liquor.
25434103	5	39	theme	process	740:746	arg1	efficiency					722:731	The efficiency	718:731	The efficiency of the process	718:746	The efficiency of the process was evaluated on the basis of production of the total reducing sugars released during the process.
25434103	7	40	theme	enzymatic	1056:1064	arg1	hydrolysis					1066:1075	enzymatic hydrolysis	1056:1075	enzymatic hydrolysis	1056:1075	The biomass pretreated with the optimized conditions could yield 0.408 g of reducing sugars /g of pretreated biomass upon enzymatic hydrolysis.
25434103	7	41	theme	optimized	966:974	arg1	conditions					976:985	the optimized conditions	962:985	the optimized conditions	962:985	The biomass pretreated with the optimized conditions could yield 0.408 g of reducing sugars /g of pretreated biomass upon enzymatic hydrolysis.
25434103	4	42	dep	done	661:664	arg1	followed					678:685	followed	678:685	followed by enzymatic saccharification	678:715	Pretreatment studies were done with H2SO4, followed by enzymatic saccharification.
25434103	7	43	theme	pretreated	1032:1041	arg1	biomass					1043:1049	pretreated biomass	1032:1049	pretreated biomass	1032:1049	The biomass pretreated with the optimized conditions could yield 0.408 g of reducing sugars /g of pretreated biomass upon enzymatic hydrolysis.
25434103	5	44	theme	total	796:800	arg1	sugars					811:816	the total reducing sugars	792:816	the total reducing sugars released during the process	792:844	The efficiency of the process was evaluated on the basis of production of the total reducing sugars released during the process.
25434103	7	45	theme	biomass	1043:1049	arg1	/g					1026:1027	/g	1026:1027	0.408 g of reducing sugars /g of pretreated biomass	999:1049	The biomass pretreated with the optimized conditions could yield 0.408 g of reducing sugars /g of pretreated biomass upon enzymatic hydrolysis.
25434103	3	46	theme	fermentable	530:540	arg1	sugars					542:547	maximum fermentable sugars	522:547	maximum fermentable sugars	522:547	In the present study, sorghum biomass was evaluated for deriving maximum fermentable sugars by optimizing various pretreatment parameters using statistical optimization methods.
25434103	1	47	theme	renewable	242:250	arg1	energy					252:257	renewable energy	242:257	renewable energy	242:257	Sorghum is one of the commercially feasible lignocellulosic biomass and has a great potential of being sustainable feedstock for renewable energy.
25434103	0	48	theme	biomass	61:67	arg1	pretreatment					12:23	Dilute acid pretreatment	0:23	Dilute acid pretreatment	0:23	Dilute acid pretreatment and enzymatic hydrolysis of sorghum biomass for sugar recovery--a statistical approach.
25434103	0	48	theme	biomass	61:67	arg1	hydrolysis					39:48	enzymatic hydrolysis	29:48	enzymatic hydrolysis	29:48	Dilute acid pretreatment and enzymatic hydrolysis of sorghum biomass for sugar recovery--a statistical approach.
25434103	1	49	theme	feasible	148:155	arg1	biomass					173:179	the commercially feasible lignocellulosic biomass	131:179	the commercially feasible lignocellulosic biomass	131:179	Sorghum is one of the commercially feasible lignocellulosic biomass and has a great potential of being sustainable feedstock for renewable energy.
25434103	5	50	theme	production	778:787	arg1	basis					769:773	the basis	765:773	the basis of production of the total reducing sugars released during the process	765:844	The efficiency of the process was evaluated on the basis of production of the total reducing sugars released during the process.
25880659	10	0	used	used	1189:1192	arg2	evaluation					1131:1140	The evaluation	1127:1140	The evaluation of monosaccharide compositions and proportions	1127:1187	The evaluation of monosaccharide compositions and proportions used gas chromatogram.
25880659	19	1	theme	radical	2416:2422	arg1	radicals					2465:2472	hydroxyl radical and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals	2407:2472	hydroxyl radical and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals	2407:2472	Compared with MPS, MZPS showed in vitro positive rising of reducing power and certain scavenging effects on hydroxyl radical and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals.
25880659	8	2	dep	polysaccharides	847:861	arg1	Mycelia					839:845	Mycelia	839:845	Mycelia	839:845	METHODS Mycelia polysaccharides (MPS) and mycelia zinc polysaccharides (MZPS) of Lentinus edodes SD-08 were extracted by hot water leaching and purified by DEAE-52 cellulose anion-exchange column chromatography separately.
25880659	21	3	theme	edodes	2924:2929	arg1	MZPS					2913:2916	the MZPS	2909:2916	the MZPS of L. edodes SD-08	2909:2935	CONCLUSIONS MZPS effectively showed potential anti-aging activities in vivo and antioxidant activities in vitro, and the molecular constituents, chemical bonds and functional groups of MZPS were superior to MPS, suggesting that the MZPS of L. edodes SD-08 could be used as a potential natural antioxidant.
25880659	21	3	theme	edodes	2924:2929	arg1	antioxidant					2974:2984	a potential natural antioxidant	2954:2984	a potential natural antioxidant	2954:2984	CONCLUSIONS MZPS effectively showed potential anti-aging activities in vivo and antioxidant activities in vitro, and the molecular constituents, chemical bonds and functional groups of MZPS were superior to MPS, suggesting that the MZPS of L. edodes SD-08 could be used as a potential natural antioxidant.
25880659	7	4	theme	present	799:805	arg1	studies					807:813	the present studies	795:813	the present studies	795:813	To investigate the bioconversion of zinc and the physiological effects of their complex (MZPS), the present studies were processed.
25880659	8	5	theme	edodes	921:926	arg1	METHODS					831:837	METHODS	831:837	METHODS Mycelia polysaccharides (MPS) and mycelia zinc polysaccharides (MZPS) of Lentinus edodes SD-08	831:932	METHODS Mycelia polysaccharides (MPS) and mycelia zinc polysaccharides (MZPS) of Lentinus edodes SD-08 were extracted by hot water leaching and purified by DEAE-52 cellulose anion-exchange column chromatography separately.
25880659	19	6	theme	hydroxyl	2407:2414	arg1	radical					2416:2422	hydroxyl radical	2407:2422	hydroxyl radical	2407:2422	Compared with MPS, MZPS showed in vitro positive rising of reducing power and certain scavenging effects on hydroxyl radical and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals.
25880659	20	7	theme	anti-aging	2517:2526	arg1	activities					2528:2537	the anti-aging activities	2513:2537	the anti-aging activities of total antioxidant capacity (T-AOC), GSH peroxide (GSH-Px), superoxide dismutase (SOD),	2513:2627	MZPS were found to upregulate in vivo the anti-aging activities of total antioxidant capacity (T-AOC), GSH peroxide (GSH-Px), superoxide dismutase (SOD), and decrease the contents of malondialdehyde (MDA).
25880659	1	8	theme	major	163:167	arg1	problem					169:175	a major problem	161:175	a major problem	161:175	BACKGROUND In the modern society, aging had been a major problem.
25880659	1	8	theme	major	163:167	arg1	BACKGROUND					112:121	BACKGROUND	112:121	BACKGROUND In the modern society, aging	112:150	BACKGROUND In the modern society, aging had been a major problem.
25880659	13	9	dep	in	1367:1368	arg1	vitro					1370:1374	vitro	1370:1374	vitro	1370:1374	The antioxidant activities in vitro measured through reducing power, the scavenging effects on hydroxyl radical and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals.
25880659	21	10	theme	CONCLUSIONS	2681:2691	arg1	MZPS					2693:2696	CONCLUSIONS MZPS	2681:2696	CONCLUSIONS MZPS	2681:2696	CONCLUSIONS MZPS effectively showed potential anti-aging activities in vivo and antioxidant activities in vitro, and the molecular constituents, chemical bonds and functional groups of MZPS were superior to MPS, suggesting that the MZPS of L. edodes SD-08 could be used as a potential natural antioxidant.
25880659	16	11	theme	proportion	2065:2074	arg1	7.19:2.26:1.00:8.39					2078:2096	molar proportion = 7.19:2.26:1.00:8.39	2059:2096	molar proportion = 7.19:2.26:1.00:8.39	2059:2096	In addition, MPS composing of rhamnose, arabinose and mannose (molar proportion = 1.75:1.00:3.02) and MZPS containing rhamnose, arabinose, mannose and glucose (molar proportion = 7.19:2.26:1.00:8.39) were investigated by gas chromatography.
25880659	16	11	theme	proportion	2065:2074	arg1	rhamnose					2017:2024	rhamnose	2017:2024	rhamnose	2017:2024	In addition, MPS composing of rhamnose, arabinose and mannose (molar proportion = 1.75:1.00:3.02) and MZPS containing rhamnose, arabinose, mannose and glucose (molar proportion = 7.19:2.26:1.00:8.39) were investigated by gas chromatography.
25880659	21	12	theme	anti-aging	2727:2736	arg1	activities					2738:2747	potential anti-aging activities	2717:2747	potential anti-aging activities	2717:2747	CONCLUSIONS MZPS effectively showed potential anti-aging activities in vivo and antioxidant activities in vitro, and the molecular constituents, chemical bonds and functional groups of MZPS were superior to MPS, suggesting that the MZPS of L. edodes SD-08 could be used as a potential natural antioxidant.
25880659	3	13	theme	medicines	242:250	arg1	medicines					242:250	medicines	242:250	medicines	242:250	However, lots of medicines were chemosynthetic, and they would do a bad side-effect on human body.
25880659	3	13	theme	medicines	242:250	arg1	lots					234:237	lots	234:237	lots of medicines	234:250	However, lots of medicines were chemosynthetic, and they would do a bad side-effect on human body.
25880659	3	14	from	side-effect	297:307	arg1	body					318:321	human body	312:321	human body	312:321	However, lots of medicines were chemosynthetic, and they would do a bad side-effect on human body.
25880659	7	15	theme	complex	779:785	arg1	effects					762:768	the physiological effects	744:768	the physiological effects of their complex (MZPS)	744:792	To investigate the bioconversion of zinc and the physiological effects of their complex (MZPS), the present studies were processed.
25880659	7	15	theme	complex	779:785	arg1	bioconversion					718:730	the bioconversion	714:730	the bioconversion of zinc	714:738	To investigate the bioconversion of zinc and the physiological effects of their complex (MZPS), the present studies were processed.
25880659	4	16	theme	natural	390:396	arg1	antioxidants					398:409	producing natural antioxidants	380:409	producing natural antioxidants	380:409	Microbial sources could be used as a potential means of producing natural antioxidants.
25880659	18	17	theme	-NH3	2273:2276	arg1	absorption					2259:2268	the typical absorption	2247:2268	the typical absorption of -NH3 (+), -NH2 and -COOH	2247:2296	MPS also had the typical absorption of -NH3 (+), -NH2 and -COOH.
25880659	17	18	from	bonds	2211:2215	arg1	MZPS					2228:2231	MZPS	2228:2231	MZPS	2228:2231	Infrared spectrum analysis indicated that there were C-H, C=O and -CH2 bonds in MPS and MZPS.
25880659	17	18	from	bonds	2211:2215	arg1	MPS					2220:2222	MPS	2220:2222	MPS	2220:2222	Infrared spectrum analysis indicated that there were C-H, C=O and -CH2 bonds in MPS and MZPS.
25880659	17	19	from	C=O	2198:2200	arg1	MZPS					2228:2231	MZPS	2228:2231	MZPS	2228:2231	Infrared spectrum analysis indicated that there were C-H, C=O and -CH2 bonds in MPS and MZPS.
25880659	17	19	from	C=O	2198:2200	arg1	MPS					2220:2222	MPS	2220:2222	MPS	2220:2222	Infrared spectrum analysis indicated that there were C-H, C=O and -CH2 bonds in MPS and MZPS.
25880659	21	20	theme	natural	2966:2972	arg1	MZPS					2913:2916	the MZPS	2909:2916	the MZPS of L. edodes SD-08	2909:2935	CONCLUSIONS MZPS effectively showed potential anti-aging activities in vivo and antioxidant activities in vitro, and the molecular constituents, chemical bonds and functional groups of MZPS were superior to MPS, suggesting that the MZPS of L. edodes SD-08 could be used as a potential natural antioxidant.
25880659	21	20	theme	natural	2966:2972	arg1	antioxidant					2974:2984	a potential natural antioxidant	2954:2984	a potential natural antioxidant	2954:2984	CONCLUSIONS MZPS effectively showed potential anti-aging activities in vivo and antioxidant activities in vitro, and the molecular constituents, chemical bonds and functional groups of MZPS were superior to MPS, suggesting that the MZPS of L. edodes SD-08 could be used as a potential natural antioxidant.
25880659	17	21	from	C-H	2193:2195	arg1	MZPS					2228:2231	MZPS	2228:2231	MZPS	2228:2231	Infrared spectrum analysis indicated that there were C-H, C=O and -CH2 bonds in MPS and MZPS.
25880659	17	21	from	C-H	2193:2195	arg1	MPS					2220:2222	MPS	2220:2222	MPS	2220:2222	Infrared spectrum analysis indicated that there were C-H, C=O and -CH2 bonds in MPS and MZPS.
25880659	19	22	from	effects	2396:2402	arg1	radicals					2465:2472	hydroxyl radical and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals	2407:2472	hydroxyl radical and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals	2407:2472	Compared with MPS, MZPS showed in vitro positive rising of reducing power and certain scavenging effects on hydroxyl radical and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals.
25880659	14	23	theme	malondialdehyde	1664:1678	arg1	dismutase					1628:1636	superoxide dismutase	1617:1636	superoxide dismutase (SOD)	1617:1642	The anti-aging activities in vivo measured through the total antioxidant capacity (T-AOC), GSH peroxide (GSH-Px), superoxide dismutase (SOD) and the contents of malondialdehyde (MDA).
25880659	14	23	theme	malondialdehyde	1664:1678	arg1	contents					1652:1659	the contents	1648:1659	the contents of malondialdehyde (MDA)	1648:1684	The anti-aging activities in vivo measured through the total antioxidant capacity (T-AOC), GSH peroxide (GSH-Px), superoxide dismutase (SOD) and the contents of malondialdehyde (MDA).
25880659	14	23	theme	malondialdehyde	1664:1678	arg1	capacity					1576:1583	the total antioxidant capacity	1554:1583	the total antioxidant capacity (T-AOC)	1554:1591	The anti-aging activities in vivo measured through the total antioxidant capacity (T-AOC), GSH peroxide (GSH-Px), superoxide dismutase (SOD) and the contents of malondialdehyde (MDA).
25880659	14	23	theme	malondialdehyde	1664:1678	arg1	peroxide					1598:1605	GSH peroxide	1594:1605	GSH peroxide (GSH-Px)	1594:1614	The anti-aging activities in vivo measured through the total antioxidant capacity (T-AOC), GSH peroxide (GSH-Px), superoxide dismutase (SOD) and the contents of malondialdehyde (MDA).
25880659	14	23	theme	malondialdehyde	1664:1678	arg1	GSH-Px					1608:1613	GSH-Px	1608:1613	GSH-Px	1608:1613	The anti-aging activities in vivo measured through the total antioxidant capacity (T-AOC), GSH peroxide (GSH-Px), superoxide dismutase (SOD) and the contents of malondialdehyde (MDA).
25880659	14	23	theme	malondialdehyde	1664:1678	arg1	SOD					1639:1641	SOD	1639:1641	SOD	1639:1641	The anti-aging activities in vivo measured through the total antioxidant capacity (T-AOC), GSH peroxide (GSH-Px), superoxide dismutase (SOD) and the contents of malondialdehyde (MDA).
25880659	14	23	theme	malondialdehyde	1664:1678	arg1	T-AOC					1586:1590	T-AOC	1586:1590	T-AOC	1586:1590	The anti-aging activities in vivo measured through the total antioxidant capacity (T-AOC), GSH peroxide (GSH-Px), superoxide dismutase (SOD) and the contents of malondialdehyde (MDA).
25880659	2	24	theme	many	197:200	arg1	medicines					202:210	many medicines	197:210	many medicines	197:210	People may rely on many medicines to delay it.
25880659	5	25	theme	physiological	503:515	arg1	research					517:524	physiological research	503:524	physiological research	503:524	Lentinus edodes, commercial obtained in daily life, had recently become more attractive in physiological research.
25880659	4	26	theme	potential	361:369	arg1	sources					334:340	Microbial sources	324:340	Microbial sources	324:340	Microbial sources could be used as a potential means of producing natural antioxidants.
25880659	4	26	theme	potential	361:369	arg1	means					371:375	a potential means	359:375	a potential means of producing natural antioxidants	359:409	Microbial sources could be used as a potential means of producing natural antioxidants.
25880659	14	27	theme	GSH	1594:1596	arg1	peroxide					1598:1605	GSH peroxide	1594:1605	GSH peroxide (GSH-Px)	1594:1614	The anti-aging activities in vivo measured through the total antioxidant capacity (T-AOC), GSH peroxide (GSH-Px), superoxide dismutase (SOD) and the contents of malondialdehyde (MDA).
25880659	14	27	theme	GSH	1594:1596	arg1	GSH-Px					1608:1613	GSH-Px	1608:1613	GSH-Px	1608:1613	The anti-aging activities in vivo measured through the total antioxidant capacity (T-AOC), GSH peroxide (GSH-Px), superoxide dismutase (SOD) and the contents of malondialdehyde (MDA).
25880659	14	28	from	activities	1518:1527	arg1	vivo					1532:1535	vivo	1532:1535	vivo	1532:1535	The anti-aging activities in vivo measured through the total antioxidant capacity (T-AOC), GSH peroxide (GSH-Px), superoxide dismutase (SOD) and the contents of malondialdehyde (MDA).
25880659	20	29	theme	malondialdehyde	2658:2672	arg1	contents					2646:2653	the contents	2642:2653	the contents of malondialdehyde (MDA)	2642:2678	MZPS were found to upregulate in vivo the anti-aging activities of total antioxidant capacity (T-AOC), GSH peroxide (GSH-Px), superoxide dismutase (SOD), and decrease the contents of malondialdehyde (MDA).
25880659	15	30	theme	Lentinus	1711:1718	arg1	edodes					1720:1725	Lentinus edodes SD-08	1711:1731	Lentinus edodes SD-08	1711:1731	RESULTS MPS and MZPS of Lentinus edodes SD-08 were extracted and purified by DEAE-52 cellulose anion-exchange column chromatography separately, and four fractions (MPS-1, MPS-2, MZPS-1 and MZPS-2) were obtained.
25880659	0	31	theme	zinc	66:69	arg1	polysaccharide					71:84	mycelia zinc polysaccharide	58:84	mycelia zinc polysaccharide	58:84	Purification, characterization and anti-aging capacity of mycelia zinc polysaccharide by Lentinus edodes SD-08.
25880659	8	32	theme	water	956:960	arg1	leaching					962:969	hot water leaching	952:969	hot water leaching	952:969	METHODS Mycelia polysaccharides (MPS) and mycelia zinc polysaccharides (MZPS) of Lentinus edodes SD-08 were extracted by hot water leaching and purified by DEAE-52 cellulose anion-exchange column chromatography separately.
25880659	3	33	theme	human	312:316	arg1	body					318:321	human body	312:321	human body	312:321	However, lots of medicines were chemosynthetic, and they would do a bad side-effect on human body.
25880659	11	34	theme	HPGPC	1250:1254	arg1	chromatogram					1256:1267	HPGPC chromatogram	1250:1267	HPGPC chromatogram	1250:1267	The analysis of molecular weight used HPGPC chromatogram.
25880659	14	35	theme	antioxidant	1564:1574	arg1	T-AOC					1586:1590	T-AOC	1586:1590	T-AOC	1586:1590	The anti-aging activities in vivo measured through the total antioxidant capacity (T-AOC), GSH peroxide (GSH-Px), superoxide dismutase (SOD) and the contents of malondialdehyde (MDA).
25880659	14	35	theme	antioxidant	1564:1574	arg1	capacity					1576:1583	the total antioxidant capacity	1554:1583	the total antioxidant capacity (T-AOC)	1554:1591	The anti-aging activities in vivo measured through the total antioxidant capacity (T-AOC), GSH peroxide (GSH-Px), superoxide dismutase (SOD) and the contents of malondialdehyde (MDA).
25880659	16	36	contain	containing	2006:2015	arg2	mannose					2038:2044	mannose	2038:2044	mannose	2038:2044	In addition, MPS composing of rhamnose, arabinose and mannose (molar proportion = 1.75:1.00:3.02) and MZPS containing rhamnose, arabinose, mannose and glucose (molar proportion = 7.19:2.26:1.00:8.39) were investigated by gas chromatography.
25880659	16	36	contain	containing	2006:2015	arg2	rhamnose					2017:2024	rhamnose	2017:2024	rhamnose	2017:2024	In addition, MPS composing of rhamnose, arabinose and mannose (molar proportion = 1.75:1.00:3.02) and MZPS containing rhamnose, arabinose, mannose and glucose (molar proportion = 7.19:2.26:1.00:8.39) were investigated by gas chromatography.
25880659	16	36	contain	containing	2006:2015	arg2	glucose					2050:2056	glucose	2050:2056	glucose	2050:2056	In addition, MPS composing of rhamnose, arabinose and mannose (molar proportion = 1.75:1.00:3.02) and MZPS containing rhamnose, arabinose, mannose and glucose (molar proportion = 7.19:2.26:1.00:8.39) were investigated by gas chromatography.
25880659	16	36	contain	containing	2006:2015	arg2	7.19:2.26:1.00:8.39					2078:2096	molar proportion = 7.19:2.26:1.00:8.39	2059:2096	molar proportion = 7.19:2.26:1.00:8.39	2059:2096	In addition, MPS composing of rhamnose, arabinose and mannose (molar proportion = 1.75:1.00:3.02) and MZPS containing rhamnose, arabinose, mannose and glucose (molar proportion = 7.19:2.26:1.00:8.39) were investigated by gas chromatography.
25880659	16	36	contain	containing	2006:2015	arg1	MZPS					2001:2004	MZPS	2001:2004	MZPS containing rhamnose, arabinose, mannose and glucose (molar proportion = 7.19:2.26:1.00:8.39)	2001:2097	In addition, MPS composing of rhamnose, arabinose and mannose (molar proportion = 1.75:1.00:3.02) and MZPS containing rhamnose, arabinose, mannose and glucose (molar proportion = 7.19:2.26:1.00:8.39) were investigated by gas chromatography.
25880659	16	36	contain	containing	2006:2015	arg2	arabinose					2027:2035	arabinose	2027:2035	arabinose	2027:2035	In addition, MPS composing of rhamnose, arabinose and mannose (molar proportion = 1.75:1.00:3.02) and MZPS containing rhamnose, arabinose, mannose and glucose (molar proportion = 7.19:2.26:1.00:8.39) were investigated by gas chromatography.
25880659	17	37	theme	-CH2	2206:2209	arg1	bonds					2211:2215	-CH2 bonds	2206:2215	-CH2 bonds	2206:2215	Infrared spectrum analysis indicated that there were C-H, C=O and -CH2 bonds in MPS and MZPS.
25880659	17	38	theme	Infrared	2140:2147	arg1	analysis					2158:2165	Infrared spectrum analysis	2140:2165	Infrared spectrum analysis	2140:2165	Infrared spectrum analysis indicated that there were C-H, C=O and -CH2 bonds in MPS and MZPS.
25880659	15	39	theme	anion-exchange	1782:1795	arg1	chromatography					1804:1817	DEAE-52 cellulose anion-exchange column chromatography	1764:1817	DEAE-52 cellulose anion-exchange column chromatography	1764:1817	RESULTS MPS and MZPS of Lentinus edodes SD-08 were extracted and purified by DEAE-52 cellulose anion-exchange column chromatography separately, and four fractions (MPS-1, MPS-2, MZPS-1 and MZPS-2) were obtained.
25880659	20	40	theme	total	2542:2546	arg1	peroxide					2582:2589	GSH peroxide	2578:2589	GSH peroxide (GSH-Px)	2578:2598	MZPS were found to upregulate in vivo the anti-aging activities of total antioxidant capacity (T-AOC), GSH peroxide (GSH-Px), superoxide dismutase (SOD), and decrease the contents of malondialdehyde (MDA).
25880659	20	40	theme	total	2542:2546	arg1	dismutase					2612:2620	superoxide dismutase	2601:2620	superoxide dismutase (SOD)	2601:2626	MZPS were found to upregulate in vivo the anti-aging activities of total antioxidant capacity (T-AOC), GSH peroxide (GSH-Px), superoxide dismutase (SOD), and decrease the contents of malondialdehyde (MDA).
25880659	20	40	theme	total	2542:2546	arg1	T-AOC					2570:2574	T-AOC	2570:2574	T-AOC	2570:2574	MZPS were found to upregulate in vivo the anti-aging activities of total antioxidant capacity (T-AOC), GSH peroxide (GSH-Px), superoxide dismutase (SOD), and decrease the contents of malondialdehyde (MDA).
25880659	20	40	theme	total	2542:2546	arg1	capacity					2560:2567	total antioxidant capacity	2542:2567	total antioxidant capacity (T-AOC)	2542:2575	MZPS were found to upregulate in vivo the anti-aging activities of total antioxidant capacity (T-AOC), GSH peroxide (GSH-Px), superoxide dismutase (SOD), and decrease the contents of malondialdehyde (MDA).
25880659	15	41	dep	RESULTS	1687:1693	arg1	RESULTS					1687:1693	RESULTS MPS and MZPS	1687:1706	RESULTS MPS and MZPS of Lentinus edodes SD-08	1687:1731	RESULTS MPS and MZPS of Lentinus edodes SD-08 were extracted and purified by DEAE-52 cellulose anion-exchange column chromatography separately, and four fractions (MPS-1, MPS-2, MZPS-1 and MZPS-2) were obtained.
25880659	15	41	dep	RESULTS	1687:1693	arg1	MZPS					1703:1706	MZPS	1703:1706	MZPS	1703:1706	RESULTS MPS and MZPS of Lentinus edodes SD-08 were extracted and purified by DEAE-52 cellulose anion-exchange column chromatography separately, and four fractions (MPS-1, MPS-2, MZPS-1 and MZPS-2) were obtained.
25880659	15	41	dep	RESULTS	1687:1693	arg1	MPS					1695:1697	MPS	1695:1697	MPS	1695:1697	RESULTS MPS and MZPS of Lentinus edodes SD-08 were extracted and purified by DEAE-52 cellulose anion-exchange column chromatography separately, and four fractions (MPS-1, MPS-2, MZPS-1 and MZPS-2) were obtained.
25880659	12	42	theme	typical	1274:1280	arg1	structure					1282:1290	The typical structure	1270:1290	The typical structure of polysaccharide	1270:1308	The typical structure of polysaccharide was evaluated by IR spectrum.
25880659	16	43	theme	molar	2059:2063	arg1	7.19:2.26:1.00:8.39					2078:2096	molar proportion = 7.19:2.26:1.00:8.39	2059:2096	molar proportion = 7.19:2.26:1.00:8.39	2059:2096	In addition, MPS composing of rhamnose, arabinose and mannose (molar proportion = 1.75:1.00:3.02) and MZPS containing rhamnose, arabinose, mannose and glucose (molar proportion = 7.19:2.26:1.00:8.39) were investigated by gas chromatography.
25880659	16	43	theme	molar	2059:2063	arg1	rhamnose					2017:2024	rhamnose	2017:2024	rhamnose	2017:2024	In addition, MPS composing of rhamnose, arabinose and mannose (molar proportion = 1.75:1.00:3.02) and MZPS containing rhamnose, arabinose, mannose and glucose (molar proportion = 7.19:2.26:1.00:8.39) were investigated by gas chromatography.
25880659	19	44	theme	in	2330:2331	arg1	rising					2348:2353	in vitro positive rising	2330:2353	in vitro positive rising of reducing power	2330:2371	Compared with MPS, MZPS showed in vitro positive rising of reducing power and certain scavenging effects on hydroxyl radical and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals.
25880659	8	45	theme	cellulose	995:1003	arg1	chromatography					1027:1040	DEAE-52 cellulose anion-exchange column chromatography	987:1040	DEAE-52 cellulose anion-exchange column chromatography	987:1040	METHODS Mycelia polysaccharides (MPS) and mycelia zinc polysaccharides (MZPS) of Lentinus edodes SD-08 were extracted by hot water leaching and purified by DEAE-52 cellulose anion-exchange column chromatography separately.
25880659	16	46	theme	=	1979:1979	arg1	1.75:1.00:3.02					1981:1994	molar proportion = 1.75:1.00:3.02	1962:1994	molar proportion = 1.75:1.00:3.02	1962:1994	In addition, MPS composing of rhamnose, arabinose and mannose (molar proportion = 1.75:1.00:3.02) and MZPS containing rhamnose, arabinose, mannose and glucose (molar proportion = 7.19:2.26:1.00:8.39) were investigated by gas chromatography.
25880659	16	46	theme	=	1979:1979	arg1	rhamnose					1929:1936	rhamnose	1929:1936	rhamnose	1929:1936	In addition, MPS composing of rhamnose, arabinose and mannose (molar proportion = 1.75:1.00:3.02) and MZPS containing rhamnose, arabinose, mannose and glucose (molar proportion = 7.19:2.26:1.00:8.39) were investigated by gas chromatography.
25880659	9	47	theme	zinc	1058:1061	arg1	content					1063:1069	The zinc content	1054:1069	The zinc content	1054:1069	The zinc content was determined by flame atomic absorption spectrometry.
25880659	7	48	theme	physiological	748:760	arg1	effects					762:768	the physiological effects	744:768	the physiological effects of their complex (MZPS)	744:792	To investigate the bioconversion of zinc and the physiological effects of their complex (MZPS), the present studies were processed.
25880659	8	49	theme	column	1020:1025	arg1	chromatography					1027:1040	DEAE-52 cellulose anion-exchange column chromatography	987:1040	DEAE-52 cellulose anion-exchange column chromatography	987:1040	METHODS Mycelia polysaccharides (MPS) and mycelia zinc polysaccharides (MZPS) of Lentinus edodes SD-08 were extracted by hot water leaching and purified by DEAE-52 cellulose anion-exchange column chromatography separately.
25880659	6	50	theme	organism	612:619	arg1	correct					586:592	correct	586:592	correct	586:592	Zinc was now considered as a major element in assuring the correct functioning of an organism and essential for maintaining coordination of the major homeostatic networks.
25880659	0	51	theme	anti-aging	35:44	arg1	capacity					46:53	anti-aging capacity	35:53	anti-aging capacity	35:53	Purification, characterization and anti-aging capacity of mycelia zinc polysaccharide by Lentinus edodes SD-08.
25880659	11	52	theme	weight	1238:1243	arg1	analysis					1216:1223	The analysis	1212:1223	The analysis of molecular weight	1212:1243	The analysis of molecular weight used HPGPC chromatogram.
25880659	20	53	theme	capacity	2560:2567	arg1	activities					2528:2537	the anti-aging activities	2513:2537	the anti-aging activities of total antioxidant capacity (T-AOC), GSH peroxide (GSH-Px), superoxide dismutase (SOD),	2513:2627	MZPS were found to upregulate in vivo the anti-aging activities of total antioxidant capacity (T-AOC), GSH peroxide (GSH-Px), superoxide dismutase (SOD), and decrease the contents of malondialdehyde (MDA).
25880659	13	54	theme	hydroxyl	1435:1442	arg1	radical					1444:1450	hydroxyl radical	1435:1450	hydroxyl radical	1435:1450	The antioxidant activities in vitro measured through reducing power, the scavenging effects on hydroxyl radical and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals.
25880659	13	54	theme	hydroxyl	1435:1442	arg1	DPPH					1487:1490	DPPH	1487:1490	DPPH	1487:1490	The antioxidant activities in vitro measured through reducing power, the scavenging effects on hydroxyl radical and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals.
25880659	19	55	theme	positive	2339:2346	arg1	rising					2348:2353	in vitro positive rising	2330:2353	in vitro positive rising of reducing power	2330:2371	Compared with MPS, MZPS showed in vitro positive rising of reducing power and certain scavenging effects on hydroxyl radical and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals.
25880659	8	56	theme	zinc	881:884	arg1	MZPS					903:906	MZPS	903:906	MZPS	903:906	METHODS Mycelia polysaccharides (MPS) and mycelia zinc polysaccharides (MZPS) of Lentinus edodes SD-08 were extracted by hot water leaching and purified by DEAE-52 cellulose anion-exchange column chromatography separately.
25880659	8	56	theme	zinc	881:884	arg1	polysaccharides					886:900	mycelia zinc polysaccharides	873:900	mycelia zinc polysaccharides (MZPS)	873:907	METHODS Mycelia polysaccharides (MPS) and mycelia zinc polysaccharides (MZPS) of Lentinus edodes SD-08 were extracted by hot water leaching and purified by DEAE-52 cellulose anion-exchange column chromatography separately.
25880659	9	57	theme	atomic	1095:1100	arg1	spectrometry					1113:1124	flame atomic absorption spectrometry	1089:1124	flame atomic absorption spectrometry	1089:1124	The zinc content was determined by flame atomic absorption spectrometry.
25880659	1	58	from	BACKGROUND	112:121	arg1	society					137:143	society	137:143	society	137:143	BACKGROUND In the modern society, aging had been a major problem.
25880659	10	59	theme	compositions	1160:1171	arg1	evaluation					1131:1140	The evaluation	1127:1140	The evaluation of monosaccharide compositions and proportions	1127:1187	The evaluation of monosaccharide compositions and proportions used gas chromatogram.
25880659	18	60	contain	had	2243:2245	arg2	absorption					2259:2268	the typical absorption	2247:2268	the typical absorption of -NH3 (+), -NH2 and -COOH	2247:2296	MPS also had the typical absorption of -NH3 (+), -NH2 and -COOH.
25880659	18	60	contain	had	2243:2245	arg1	MPS					2234:2236	MPS	2234:2236	MPS	2234:2236	MPS also had the typical absorption of -NH3 (+), -NH2 and -COOH.
25880659	4	61	used	used	351:354	arg2	means					371:375	a potential means	359:375	a potential means of producing natural antioxidants	359:409	Microbial sources could be used as a potential means of producing natural antioxidants.
25880659	4	61	used	used	351:354	arg2	sources					334:340	Microbial sources	324:340	Microbial sources	324:340	Microbial sources could be used as a potential means of producing natural antioxidants.
25880659	21	62	used	used	2946:2949	arg2	antioxidant					2974:2984	a potential natural antioxidant	2954:2984	a potential natural antioxidant	2954:2984	CONCLUSIONS MZPS effectively showed potential anti-aging activities in vivo and antioxidant activities in vitro, and the molecular constituents, chemical bonds and functional groups of MZPS were superior to MPS, suggesting that the MZPS of L. edodes SD-08 could be used as a potential natural antioxidant.
25880659	21	62	used	used	2946:2949	arg2	MZPS					2913:2916	the MZPS	2909:2916	the MZPS of L. edodes SD-08	2909:2935	CONCLUSIONS MZPS effectively showed potential anti-aging activities in vivo and antioxidant activities in vitro, and the molecular constituents, chemical bonds and functional groups of MZPS were superior to MPS, suggesting that the MZPS of L. edodes SD-08 could be used as a potential natural antioxidant.
25880659	21	63	theme	chemical	2826:2833	arg1	bonds					2835:2839	chemical bonds	2826:2839	chemical bonds	2826:2839	CONCLUSIONS MZPS effectively showed potential anti-aging activities in vivo and antioxidant activities in vitro, and the molecular constituents, chemical bonds and functional groups of MZPS were superior to MPS, suggesting that the MZPS of L. edodes SD-08 could be used as a potential natural antioxidant.
25880659	6	64	theme	networks	689:696	arg1	coordination					651:662	coordination	651:662	coordination of the major homeostatic networks	651:696	Zinc was now considered as a major element in assuring the correct functioning of an organism and essential for maintaining coordination of the major homeostatic networks.
25880659	5	65	theme	Lentinus	412:419	arg1	commercial					429:438	commercial	429:438	commercial	429:438	Lentinus edodes, commercial obtained in daily life, had recently become more attractive in physiological research.
25880659	5	65	theme	Lentinus	412:419	arg1	edodes					421:426	Lentinus edodes	412:426	Lentinus edodes	412:426	Lentinus edodes, commercial obtained in daily life, had recently become more attractive in physiological research.
25880659	19	66	theme	power	2367:2371	arg1	rising					2348:2353	in vitro positive rising	2330:2353	in vitro positive rising of reducing power	2330:2371	Compared with MPS, MZPS showed in vitro positive rising of reducing power and certain scavenging effects on hydroxyl radical and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals.
25880659	19	66	theme	power	2367:2371	arg1	effects					2396:2402	certain scavenging effects	2377:2402	certain scavenging effects on hydroxyl radical and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals	2377:2472	Compared with MPS, MZPS showed in vitro positive rising of reducing power and certain scavenging effects on hydroxyl radical and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals.
25880659	16	67	theme	=	2076:2076	arg1	7.19:2.26:1.00:8.39					2078:2096	molar proportion = 7.19:2.26:1.00:8.39	2059:2096	molar proportion = 7.19:2.26:1.00:8.39	2059:2096	In addition, MPS composing of rhamnose, arabinose and mannose (molar proportion = 1.75:1.00:3.02) and MZPS containing rhamnose, arabinose, mannose and glucose (molar proportion = 7.19:2.26:1.00:8.39) were investigated by gas chromatography.
25880659	16	67	theme	=	2076:2076	arg1	rhamnose					2017:2024	rhamnose	2017:2024	rhamnose	2017:2024	In addition, MPS composing of rhamnose, arabinose and mannose (molar proportion = 1.75:1.00:3.02) and MZPS containing rhamnose, arabinose, mannose and glucose (molar proportion = 7.19:2.26:1.00:8.39) were investigated by gas chromatography.
25880659	6	68	theme	major	671:675	arg1	networks					689:696	the major homeostatic networks	667:696	the major homeostatic networks	667:696	Zinc was now considered as a major element in assuring the correct functioning of an organism and essential for maintaining coordination of the major homeostatic networks.
25880659	10	69	theme	gas	1194:1196	arg1	chromatogram					1198:1209	gas chromatogram	1194:1209	gas chromatogram	1194:1209	The evaluation of monosaccharide compositions and proportions used gas chromatogram.
25880659	10	70	theme	proportions	1177:1187	arg1	evaluation					1131:1140	The evaluation	1127:1140	The evaluation of monosaccharide compositions and proportions	1127:1187	The evaluation of monosaccharide compositions and proportions used gas chromatogram.
25880659	19	71	dep	in	2330:2331	arg1	vitro					2333:2337	vitro	2333:2337	vitro	2333:2337	Compared with MPS, MZPS showed in vitro positive rising of reducing power and certain scavenging effects on hydroxyl radical and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals.
25880659	15	72	theme	column	1797:1802	arg1	chromatography					1804:1817	DEAE-52 cellulose anion-exchange column chromatography	1764:1817	DEAE-52 cellulose anion-exchange column chromatography	1764:1817	RESULTS MPS and MZPS of Lentinus edodes SD-08 were extracted and purified by DEAE-52 cellulose anion-exchange column chromatography separately, and four fractions (MPS-1, MPS-2, MZPS-1 and MZPS-2) were obtained.
25880659	21	73	theme	MZPS	2866:2869	arg1	MZPS					2866:2869	MZPS	2866:2869	MZPS	2866:2869	CONCLUSIONS MZPS effectively showed potential anti-aging activities in vivo and antioxidant activities in vitro, and the molecular constituents, chemical bonds and functional groups of MZPS were superior to MPS, suggesting that the MZPS of L. edodes SD-08 could be used as a potential natural antioxidant.
25880659	21	73	theme	MZPS	2866:2869	arg1	superior					2876:2883	superior	2876:2883	superior	2876:2883	CONCLUSIONS MZPS effectively showed potential anti-aging activities in vivo and antioxidant activities in vitro, and the molecular constituents, chemical bonds and functional groups of MZPS were superior to MPS, suggesting that the MZPS of L. edodes SD-08 could be used as a potential natural antioxidant.
25880659	21	73	theme	MZPS	2866:2869	arg1	constituents					2812:2823	the molecular constituents	2798:2823	the molecular constituents	2798:2823	CONCLUSIONS MZPS effectively showed potential anti-aging activities in vivo and antioxidant activities in vitro, and the molecular constituents, chemical bonds and functional groups of MZPS were superior to MPS, suggesting that the MZPS of L. edodes SD-08 could be used as a potential natural antioxidant.
25880659	21	73	theme	MZPS	2866:2869	arg1	bonds					2835:2839	chemical bonds	2826:2839	chemical bonds	2826:2839	CONCLUSIONS MZPS effectively showed potential anti-aging activities in vivo and antioxidant activities in vitro, and the molecular constituents, chemical bonds and functional groups of MZPS were superior to MPS, suggesting that the MZPS of L. edodes SD-08 could be used as a potential natural antioxidant.
25880659	21	73	theme	MZPS	2866:2869	arg1	groups					2856:2861	functional groups	2845:2861	functional groups of MZPS	2845:2869	CONCLUSIONS MZPS effectively showed potential anti-aging activities in vivo and antioxidant activities in vitro, and the molecular constituents, chemical bonds and functional groups of MZPS were superior to MPS, suggesting that the MZPS of L. edodes SD-08 could be used as a potential natural antioxidant.
25880659	5	74	theme	daily	452:456	arg1	life					458:461	daily life	452:461	daily life	452:461	Lentinus edodes, commercial obtained in daily life, had recently become more attractive in physiological research.
25880659	4	75	theme	Microbial	324:332	arg1	sources					334:340	Microbial sources	324:340	Microbial sources	324:340	Microbial sources could be used as a potential means of producing natural antioxidants.
25880659	4	75	theme	Microbial	324:332	arg1	means					371:375	a potential means	359:375	a potential means of producing natural antioxidants	359:409	Microbial sources could be used as a potential means of producing natural antioxidants.
25880659	6	76	theme	major	556:560	arg1	element					562:568	a major element	554:568	a major element in assuring the correct functioning of an organism and essential for maintaining coordination of the major homeostatic networks	554:696	Zinc was now considered as a major element in assuring the correct functioning of an organism and essential for maintaining coordination of the major homeostatic networks.
25880659	6	76	theme	major	556:560	arg1	Zinc					527:530	Zinc	527:530	Zinc	527:530	Zinc was now considered as a major element in assuring the correct functioning of an organism and essential for maintaining coordination of the major homeostatic networks.
25880659	8	77	theme	hot	952:954	arg1	leaching					962:969	hot water leaching	952:969	hot water leaching	952:969	METHODS Mycelia polysaccharides (MPS) and mycelia zinc polysaccharides (MZPS) of Lentinus edodes SD-08 were extracted by hot water leaching and purified by DEAE-52 cellulose anion-exchange column chromatography separately.
25880659	4	78	theme	antioxidants	398:409	arg1	sources					334:340	Microbial sources	324:340	Microbial sources	324:340	Microbial sources could be used as a potential means of producing natural antioxidants.
25880659	4	78	theme	antioxidants	398:409	arg1	means					371:375	a potential means	359:375	a potential means of producing natural antioxidants	359:409	Microbial sources could be used as a potential means of producing natural antioxidants.
25880659	15	79	dep	fractions	1840:1848	arg1	MPS-2					1858:1862	MPS-2	1858:1862	MPS-2	1858:1862	RESULTS MPS and MZPS of Lentinus edodes SD-08 were extracted and purified by DEAE-52 cellulose anion-exchange column chromatography separately, and four fractions (MPS-1, MPS-2, MZPS-1 and MZPS-2) were obtained.
25880659	15	79	dep	fractions	1840:1848	arg1	fractions					1840:1848	four fractions	1835:1848	four fractions (MPS-1, MPS-2, MZPS-1 and MZPS-2)	1835:1882	RESULTS MPS and MZPS of Lentinus edodes SD-08 were extracted and purified by DEAE-52 cellulose anion-exchange column chromatography separately, and four fractions (MPS-1, MPS-2, MZPS-1 and MZPS-2) were obtained.
25880659	15	79	dep	fractions	1840:1848	arg1	MPS-1					1851:1855	MPS-1	1851:1855	MPS-1	1851:1855	RESULTS MPS and MZPS of Lentinus edodes SD-08 were extracted and purified by DEAE-52 cellulose anion-exchange column chromatography separately, and four fractions (MPS-1, MPS-2, MZPS-1 and MZPS-2) were obtained.
25880659	15	79	dep	fractions	1840:1848	arg1	MZPS-2					1876:1881	MZPS-2	1876:1881	MZPS-2	1876:1881	RESULTS MPS and MZPS of Lentinus edodes SD-08 were extracted and purified by DEAE-52 cellulose anion-exchange column chromatography separately, and four fractions (MPS-1, MPS-2, MZPS-1 and MZPS-2) were obtained.
25880659	15	79	dep	fractions	1840:1848	arg1	MZPS-1					1865:1870	MZPS-1	1865:1870	MZPS-1	1865:1870	RESULTS MPS and MZPS of Lentinus edodes SD-08 were extracted and purified by DEAE-52 cellulose anion-exchange column chromatography separately, and four fractions (MPS-1, MPS-2, MZPS-1 and MZPS-2) were obtained.
25880659	19	80	theme	DPPH	2459:2462	arg1	radicals					2465:2472	hydroxyl radical and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals	2407:2472	hydroxyl radical and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals	2407:2472	Compared with MPS, MZPS showed in vitro positive rising of reducing power and certain scavenging effects on hydroxyl radical and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals.
25880659	21	81	theme	potential	2956:2964	arg1	MZPS					2913:2916	the MZPS	2909:2916	the MZPS of L. edodes SD-08	2909:2935	CONCLUSIONS MZPS effectively showed potential anti-aging activities in vivo and antioxidant activities in vitro, and the molecular constituents, chemical bonds and functional groups of MZPS were superior to MPS, suggesting that the MZPS of L. edodes SD-08 could be used as a potential natural antioxidant.
25880659	21	81	theme	potential	2956:2964	arg1	antioxidant					2974:2984	a potential natural antioxidant	2954:2984	a potential natural antioxidant	2954:2984	CONCLUSIONS MZPS effectively showed potential anti-aging activities in vivo and antioxidant activities in vitro, and the molecular constituents, chemical bonds and functional groups of MZPS were superior to MPS, suggesting that the MZPS of L. edodes SD-08 could be used as a potential natural antioxidant.
25880659	16	82	theme	gas	2120:2122	arg1	chromatography					2124:2137	gas chromatography	2120:2137	gas chromatography	2120:2137	In addition, MPS composing of rhamnose, arabinose and mannose (molar proportion = 1.75:1.00:3.02) and MZPS containing rhamnose, arabinose, mannose and glucose (molar proportion = 7.19:2.26:1.00:8.39) were investigated by gas chromatography.
25880659	13	83	theme	in	1367:1368	arg1	activities					1356:1365	The antioxidant activities	1340:1365	The antioxidant activities in vitro	1340:1374	The antioxidant activities in vitro measured through reducing power, the scavenging effects on hydroxyl radical and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals.
25880659	19	84	theme	1,1-diphenyl-2-picrylhydrazyl	2428:2456	arg1	radicals					2465:2472	hydroxyl radical and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals	2407:2472	hydroxyl radical and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals	2407:2472	Compared with MPS, MZPS showed in vitro positive rising of reducing power and certain scavenging effects on hydroxyl radical and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals.
25880659	18	85	theme	typical	2251:2257	arg1	absorption					2259:2268	the typical absorption	2247:2268	the typical absorption of -NH3 (+), -NH2 and -COOH	2247:2296	MPS also had the typical absorption of -NH3 (+), -NH2 and -COOH.
25880659	15	86	theme	edodes	1720:1725	arg1	RESULTS					1687:1693	RESULTS MPS and MZPS	1687:1706	RESULTS MPS and MZPS of Lentinus edodes SD-08	1687:1731	RESULTS MPS and MZPS of Lentinus edodes SD-08 were extracted and purified by DEAE-52 cellulose anion-exchange column chromatography separately, and four fractions (MPS-1, MPS-2, MZPS-1 and MZPS-2) were obtained.
25880659	15	86	theme	edodes	1720:1725	arg1	MZPS					1703:1706	MZPS	1703:1706	MZPS	1703:1706	RESULTS MPS and MZPS of Lentinus edodes SD-08 were extracted and purified by DEAE-52 cellulose anion-exchange column chromatography separately, and four fractions (MPS-1, MPS-2, MZPS-1 and MZPS-2) were obtained.
25880659	15	86	theme	edodes	1720:1725	arg1	MPS					1695:1697	MPS	1695:1697	MPS	1695:1697	RESULTS MPS and MZPS of Lentinus edodes SD-08 were extracted and purified by DEAE-52 cellulose anion-exchange column chromatography separately, and four fractions (MPS-1, MPS-2, MZPS-1 and MZPS-2) were obtained.
25880659	21	87	theme	potential	2717:2725	arg1	activities					2738:2747	potential anti-aging activities	2717:2747	potential anti-aging activities	2717:2747	CONCLUSIONS MZPS effectively showed potential anti-aging activities in vivo and antioxidant activities in vitro, and the molecular constituents, chemical bonds and functional groups of MZPS were superior to MPS, suggesting that the MZPS of L. edodes SD-08 could be used as a potential natural antioxidant.
25880659	4	88	theme	producing	380:388	arg1	antioxidants					398:409	producing natural antioxidants	380:409	producing natural antioxidants	380:409	Microbial sources could be used as a potential means of producing natural antioxidants.
25880659	0	89	theme	polysaccharide	71:84	arg1	characterization					14:29	characterization	14:29	characterization	14:29	Purification, characterization and anti-aging capacity of mycelia zinc polysaccharide by Lentinus edodes SD-08.
25880659	0	89	theme	polysaccharide	71:84	arg1	capacity					46:53	anti-aging capacity	35:53	anti-aging capacity	35:53	Purification, characterization and anti-aging capacity of mycelia zinc polysaccharide by Lentinus edodes SD-08.
25880659	0	89	theme	polysaccharide	71:84	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification, characterization and anti-aging capacity of mycelia zinc polysaccharide by Lentinus edodes SD-08.
25880659	3	90	theme	bad	293:295	arg1	side-effect					297:307	a bad side-effect	291:307	a bad side-effect on human body	291:321	However, lots of medicines were chemosynthetic, and they would do a bad side-effect on human body.
25880659	19	91	theme	certain	2377:2383	arg1	effects					2396:2402	certain scavenging effects	2377:2402	certain scavenging effects on hydroxyl radical and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals	2377:2472	Compared with MPS, MZPS showed in vitro positive rising of reducing power and certain scavenging effects on hydroxyl radical and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals.
25880659	13	92	theme	antioxidant	1344:1354	arg1	activities					1356:1365	The antioxidant activities	1340:1365	The antioxidant activities in vitro	1340:1374	The antioxidant activities in vitro measured through reducing power, the scavenging effects on hydroxyl radical and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals.
25880659	19	93	from	rising	2348:2353	arg1	radicals					2465:2472	hydroxyl radical and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals	2407:2472	hydroxyl radical and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals	2407:2472	Compared with MPS, MZPS showed in vitro positive rising of reducing power and certain scavenging effects on hydroxyl radical and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals.
25880659	13	94	from	effects	1424:1430	arg1	radicals					1493:1500	hydroxyl radical and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals	1435:1500	hydroxyl radical and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals	1435:1500	The antioxidant activities in vitro measured through reducing power, the scavenging effects on hydroxyl radical and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals.
25880659	21	95	theme	antioxidant	2761:2771	arg1	activities					2773:2782	antioxidant activities	2761:2782	antioxidant activities	2761:2782	CONCLUSIONS MZPS effectively showed potential anti-aging activities in vivo and antioxidant activities in vitro, and the molecular constituents, chemical bonds and functional groups of MZPS were superior to MPS, suggesting that the MZPS of L. edodes SD-08 could be used as a potential natural antioxidant.
25880659	11	96	used	used	1245:1248	arg2	analysis					1216:1223	The analysis	1212:1223	The analysis of molecular weight	1212:1243	The analysis of molecular weight used HPGPC chromatogram.
25880659	14	97	theme	total	1558:1562	arg1	T-AOC					1586:1590	T-AOC	1586:1590	T-AOC	1586:1590	The anti-aging activities in vivo measured through the total antioxidant capacity (T-AOC), GSH peroxide (GSH-Px), superoxide dismutase (SOD) and the contents of malondialdehyde (MDA).
25880659	14	97	theme	total	1558:1562	arg1	capacity					1576:1583	the total antioxidant capacity	1554:1583	the total antioxidant capacity (T-AOC)	1554:1591	The anti-aging activities in vivo measured through the total antioxidant capacity (T-AOC), GSH peroxide (GSH-Px), superoxide dismutase (SOD) and the contents of malondialdehyde (MDA).
25880659	1	98	dep	society	137:143	arg1	aging					146:150	aging	146:150	aging	146:150	BACKGROUND In the modern society, aging had been a major problem.
25880659	15	99	theme	cellulose	1772:1780	arg1	chromatography					1804:1817	DEAE-52 cellulose anion-exchange column chromatography	1764:1817	DEAE-52 cellulose anion-exchange column chromatography	1764:1817	RESULTS MPS and MZPS of Lentinus edodes SD-08 were extracted and purified by DEAE-52 cellulose anion-exchange column chromatography separately, and four fractions (MPS-1, MPS-2, MZPS-1 and MZPS-2) were obtained.
25880659	20	100	theme	antioxidant	2548:2558	arg1	peroxide					2582:2589	GSH peroxide	2578:2589	GSH peroxide (GSH-Px)	2578:2598	MZPS were found to upregulate in vivo the anti-aging activities of total antioxidant capacity (T-AOC), GSH peroxide (GSH-Px), superoxide dismutase (SOD), and decrease the contents of malondialdehyde (MDA).
25880659	20	100	theme	antioxidant	2548:2558	arg1	dismutase					2612:2620	superoxide dismutase	2601:2620	superoxide dismutase (SOD)	2601:2626	MZPS were found to upregulate in vivo the anti-aging activities of total antioxidant capacity (T-AOC), GSH peroxide (GSH-Px), superoxide dismutase (SOD), and decrease the contents of malondialdehyde (MDA).
25880659	20	100	theme	antioxidant	2548:2558	arg1	T-AOC					2570:2574	T-AOC	2570:2574	T-AOC	2570:2574	MZPS were found to upregulate in vivo the anti-aging activities of total antioxidant capacity (T-AOC), GSH peroxide (GSH-Px), superoxide dismutase (SOD), and decrease the contents of malondialdehyde (MDA).
25880659	20	100	theme	antioxidant	2548:2558	arg1	capacity					2560:2567	total antioxidant capacity	2542:2567	total antioxidant capacity (T-AOC)	2542:2575	MZPS were found to upregulate in vivo the anti-aging activities of total antioxidant capacity (T-AOC), GSH peroxide (GSH-Px), superoxide dismutase (SOD), and decrease the contents of malondialdehyde (MDA).
25880659	14	101	theme	superoxide	1617:1626	arg1	SOD					1639:1641	SOD	1639:1641	SOD	1639:1641	The anti-aging activities in vivo measured through the total antioxidant capacity (T-AOC), GSH peroxide (GSH-Px), superoxide dismutase (SOD) and the contents of malondialdehyde (MDA).
25880659	14	101	theme	superoxide	1617:1626	arg1	dismutase					1628:1636	superoxide dismutase	1617:1636	superoxide dismutase (SOD)	1617:1642	The anti-aging activities in vivo measured through the total antioxidant capacity (T-AOC), GSH peroxide (GSH-Px), superoxide dismutase (SOD) and the contents of malondialdehyde (MDA).
25880659	8	102	theme	DEAE-52	987:993	arg1	cellulose					995:1003	cellulose	995:1003	cellulose	995:1003	METHODS Mycelia polysaccharides (MPS) and mycelia zinc polysaccharides (MZPS) of Lentinus edodes SD-08 were extracted by hot water leaching and purified by DEAE-52 cellulose anion-exchange column chromatography separately.
25880659	17	103	theme	spectrum	2149:2156	arg1	analysis					2158:2165	Infrared spectrum analysis	2140:2165	Infrared spectrum analysis	2140:2165	Infrared spectrum analysis indicated that there were C-H, C=O and -CH2 bonds in MPS and MZPS.
25880659	8	104	theme	anion-exchange	1005:1018	arg1	chromatography					1027:1040	DEAE-52 cellulose anion-exchange column chromatography	987:1040	DEAE-52 cellulose anion-exchange column chromatography	987:1040	METHODS Mycelia polysaccharides (MPS) and mycelia zinc polysaccharides (MZPS) of Lentinus edodes SD-08 were extracted by hot water leaching and purified by DEAE-52 cellulose anion-exchange column chromatography separately.
25880659	12	105	theme	polysaccharide	1295:1308	arg1	structure					1282:1290	The typical structure	1270:1290	The typical structure of polysaccharide	1270:1308	The typical structure of polysaccharide was evaluated by IR spectrum.
25880659	20	106	theme	superoxide	2601:2610	arg1	SOD					2623:2625	SOD	2623:2625	SOD	2623:2625	MZPS were found to upregulate in vivo the anti-aging activities of total antioxidant capacity (T-AOC), GSH peroxide (GSH-Px), superoxide dismutase (SOD), and decrease the contents of malondialdehyde (MDA).
25880659	20	106	theme	superoxide	2601:2610	arg1	dismutase					2612:2620	superoxide dismutase	2601:2620	superoxide dismutase (SOD)	2601:2626	MZPS were found to upregulate in vivo the anti-aging activities of total antioxidant capacity (T-AOC), GSH peroxide (GSH-Px), superoxide dismutase (SOD), and decrease the contents of malondialdehyde (MDA).
25880659	20	106	theme	superoxide	2601:2610	arg1	capacity					2560:2567	total antioxidant capacity	2542:2567	total antioxidant capacity (T-AOC)	2542:2575	MZPS were found to upregulate in vivo the anti-aging activities of total antioxidant capacity (T-AOC), GSH peroxide (GSH-Px), superoxide dismutase (SOD), and decrease the contents of malondialdehyde (MDA).
25880659	16	107	theme	molar	1962:1966	arg1	1.75:1.00:3.02					1981:1994	molar proportion = 1.75:1.00:3.02	1962:1994	molar proportion = 1.75:1.00:3.02	1962:1994	In addition, MPS composing of rhamnose, arabinose and mannose (molar proportion = 1.75:1.00:3.02) and MZPS containing rhamnose, arabinose, mannose and glucose (molar proportion = 7.19:2.26:1.00:8.39) were investigated by gas chromatography.
25880659	16	107	theme	molar	1962:1966	arg1	rhamnose					1929:1936	rhamnose	1929:1936	rhamnose	1929:1936	In addition, MPS composing of rhamnose, arabinose and mannose (molar proportion = 1.75:1.00:3.02) and MZPS containing rhamnose, arabinose, mannose and glucose (molar proportion = 7.19:2.26:1.00:8.39) were investigated by gas chromatography.
25880659	16	108	theme	proportion	1968:1977	arg1	1.75:1.00:3.02					1981:1994	molar proportion = 1.75:1.00:3.02	1962:1994	molar proportion = 1.75:1.00:3.02	1962:1994	In addition, MPS composing of rhamnose, arabinose and mannose (molar proportion = 1.75:1.00:3.02) and MZPS containing rhamnose, arabinose, mannose and glucose (molar proportion = 7.19:2.26:1.00:8.39) were investigated by gas chromatography.
25880659	16	108	theme	proportion	1968:1977	arg1	rhamnose					1929:1936	rhamnose	1929:1936	rhamnose	1929:1936	In addition, MPS composing of rhamnose, arabinose and mannose (molar proportion = 1.75:1.00:3.02) and MZPS containing rhamnose, arabinose, mannose and glucose (molar proportion = 7.19:2.26:1.00:8.39) were investigated by gas chromatography.
25880659	19	109	theme	scavenging	2385:2394	arg1	effects					2396:2402	certain scavenging effects	2377:2402	certain scavenging effects on hydroxyl radical and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals	2377:2472	Compared with MPS, MZPS showed in vitro positive rising of reducing power and certain scavenging effects on hydroxyl radical and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals.
25880659	20	110	theme	GSH	2578:2580	arg1	peroxide					2582:2589	GSH peroxide	2578:2589	GSH peroxide (GSH-Px)	2578:2598	MZPS were found to upregulate in vivo the anti-aging activities of total antioxidant capacity (T-AOC), GSH peroxide (GSH-Px), superoxide dismutase (SOD), and decrease the contents of malondialdehyde (MDA).
25880659	20	110	theme	GSH	2578:2580	arg1	capacity					2560:2567	total antioxidant capacity	2542:2567	total antioxidant capacity (T-AOC)	2542:2575	MZPS were found to upregulate in vivo the anti-aging activities of total antioxidant capacity (T-AOC), GSH peroxide (GSH-Px), superoxide dismutase (SOD), and decrease the contents of malondialdehyde (MDA).
25880659	20	110	theme	GSH	2578:2580	arg1	GSH-Px					2592:2597	GSH-Px	2592:2597	GSH-Px	2592:2597	MZPS were found to upregulate in vivo the anti-aging activities of total antioxidant capacity (T-AOC), GSH peroxide (GSH-Px), superoxide dismutase (SOD), and decrease the contents of malondialdehyde (MDA).
25880659	21	111	theme	functional	2845:2854	arg1	MZPS					2866:2869	MZPS	2866:2869	MZPS	2866:2869	CONCLUSIONS MZPS effectively showed potential anti-aging activities in vivo and antioxidant activities in vitro, and the molecular constituents, chemical bonds and functional groups of MZPS were superior to MPS, suggesting that the MZPS of L. edodes SD-08 could be used as a potential natural antioxidant.
25880659	21	111	theme	functional	2845:2854	arg1	groups					2856:2861	functional groups	2845:2861	functional groups of MZPS	2845:2869	CONCLUSIONS MZPS effectively showed potential anti-aging activities in vivo and antioxidant activities in vitro, and the molecular constituents, chemical bonds and functional groups of MZPS were superior to MPS, suggesting that the MZPS of L. edodes SD-08 could be used as a potential natural antioxidant.
25880659	0	112	theme	mycelia	58:64	arg1	polysaccharide					71:84	mycelia zinc polysaccharide	58:84	mycelia zinc polysaccharide	58:84	Purification, characterization and anti-aging capacity of mycelia zinc polysaccharide by Lentinus edodes SD-08.
25880659	19	113	theme	reducing	2358:2365	arg1	power					2367:2371	reducing power	2358:2371	reducing power	2358:2371	Compared with MPS, MZPS showed in vitro positive rising of reducing power and certain scavenging effects on hydroxyl radical and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals.
25880659	21	114	theme	molecular	2802:2810	arg1	superior					2876:2883	superior	2876:2883	superior	2876:2883	CONCLUSIONS MZPS effectively showed potential anti-aging activities in vivo and antioxidant activities in vitro, and the molecular constituents, chemical bonds and functional groups of MZPS were superior to MPS, suggesting that the MZPS of L. edodes SD-08 could be used as a potential natural antioxidant.
25880659	21	114	theme	molecular	2802:2810	arg1	constituents					2812:2823	the molecular constituents	2798:2823	the molecular constituents	2798:2823	CONCLUSIONS MZPS effectively showed potential anti-aging activities in vivo and antioxidant activities in vitro, and the molecular constituents, chemical bonds and functional groups of MZPS were superior to MPS, suggesting that the MZPS of L. edodes SD-08 could be used as a potential natural antioxidant.
25880659	9	115	theme	flame	1089:1093	arg1	spectrometry					1113:1124	flame atomic absorption spectrometry	1089:1124	flame atomic absorption spectrometry	1089:1124	The zinc content was determined by flame atomic absorption spectrometry.
25880659	13	116	theme	radical	1444:1450	arg1	radicals					1493:1500	hydroxyl radical and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals	1435:1500	hydroxyl radical and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals	1435:1500	The antioxidant activities in vitro measured through reducing power, the scavenging effects on hydroxyl radical and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals.
25880659	7	117	theme	zinc	735:738	arg1	effects					762:768	the physiological effects	744:768	the physiological effects of their complex (MZPS)	744:792	To investigate the bioconversion of zinc and the physiological effects of their complex (MZPS), the present studies were processed.
25880659	7	117	theme	zinc	735:738	arg1	bioconversion					718:730	the bioconversion	714:730	the bioconversion of zinc	714:738	To investigate the bioconversion of zinc and the physiological effects of their complex (MZPS), the present studies were processed.
25880659	8	118	theme	mycelia	873:879	arg1	MZPS					903:906	MZPS	903:906	MZPS	903:906	METHODS Mycelia polysaccharides (MPS) and mycelia zinc polysaccharides (MZPS) of Lentinus edodes SD-08 were extracted by hot water leaching and purified by DEAE-52 cellulose anion-exchange column chromatography separately.
25880659	8	118	theme	mycelia	873:879	arg1	polysaccharides					886:900	mycelia zinc polysaccharides	873:900	mycelia zinc polysaccharides (MZPS)	873:907	METHODS Mycelia polysaccharides (MPS) and mycelia zinc polysaccharides (MZPS) of Lentinus edodes SD-08 were extracted by hot water leaching and purified by DEAE-52 cellulose anion-exchange column chromatography separately.
25880659	14	119	theme	anti-aging	1507:1516	arg1	activities					1518:1527	The anti-aging activities	1503:1527	The anti-aging activities in vivo	1503:1535	The anti-aging activities in vivo measured through the total antioxidant capacity (T-AOC), GSH peroxide (GSH-Px), superoxide dismutase (SOD) and the contents of malondialdehyde (MDA).
25880659	11	120	theme	molecular	1228:1236	arg1	weight					1238:1243	molecular weight	1228:1243	molecular weight	1228:1243	The analysis of molecular weight used HPGPC chromatogram.
25880659	9	121	theme	absorption	1102:1111	arg1	spectrometry					1113:1124	flame atomic absorption spectrometry	1089:1124	flame atomic absorption spectrometry	1089:1124	The zinc content was determined by flame atomic absorption spectrometry.
25880659	13	122	theme	1,1-diphenyl-2-picrylhydrazyl	1456:1484	arg1	radicals					1493:1500	hydroxyl radical and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals	1435:1500	hydroxyl radical and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals	1435:1500	The antioxidant activities in vitro measured through reducing power, the scavenging effects on hydroxyl radical and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals.
25880659	10	123	theme	monosaccharide	1145:1158	arg1	compositions					1160:1171	monosaccharide compositions	1145:1171	monosaccharide compositions	1145:1171	The evaluation of monosaccharide compositions and proportions used gas chromatogram.
25880659	6	124	theme	homeostatic	677:687	arg1	networks					689:696	the major homeostatic networks	667:696	the major homeostatic networks	667:696	Zinc was now considered as a major element in assuring the correct functioning of an organism and essential for maintaining coordination of the major homeostatic networks.
25880659	13	125	theme	scavenging	1413:1422	arg1	effects					1424:1430	the scavenging effects	1409:1430	the scavenging effects on hydroxyl radical and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals	1409:1500	The antioxidant activities in vitro measured through reducing power, the scavenging effects on hydroxyl radical and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals.
27796438	6	0	from	protein	809:815	arg1	layer					869:873	each layer	864:873	each layer of EPS	864:880	The protein (PN) and polysaccharide (PS) concentrations in each layer of EPS decreased during the composite conditioning, especially in tightly bound EPS (TB-EPS) and slime layer EPS (S-EPS).
27796438	7	1	theme	water	1034:1038	arg1	%					1019:1019	approximately 68 %	1002:1019	approximately 68 % of the bound water	1002:1038	And, approximately 68 % of the bound water was released in this process.
27796438	7	1	theme	water	1034:1038	arg1	water					1034:1038	the bound water	1024:1038	the bound water	1024:1038	And, approximately 68 % of the bound water was released in this process.
27796438	1	2	theme	dimethyl	148:155	arg1	DDBAC					183:187	DDBAC	183:187	DDBAC	183:187	The effect of Fenton's reagent combined with dodecyl dimethyl benzyl ammonium chloride (DDBAC) on sludge dewaterability was studied.
27796438	1	2	theme	dimethyl	148:155	arg1	chloride					173:180	dodecyl dimethyl benzyl ammonium chloride	140:180	dodecyl dimethyl benzyl ammonium chloride (DDBAC)	140:188	The effect of Fenton's reagent combined with dodecyl dimethyl benzyl ammonium chloride (DDBAC) on sludge dewaterability was studied.
27796438	5	3	theme	DDBAC	712:716	arg1	dosage					686:691	the dosage	682:691	the dosage of Fe2+, H2O2, and DDBAC	682:716	When the dosage of Fe2+, H2O2, and DDBAC were 40, 40, and 60 mg g-1 at pH 4, WC of 57.17 % and CST of 17.2 s could be achieved.
27796438	5	3	theme	DDBAC	712:716	arg1	40					723:724	40	723:724	40	723:724	When the dosage of Fe2+, H2O2, and DDBAC were 40, 40, and 60 mg g-1 at pH 4, WC of 57.17 % and CST of 17.2 s could be achieved.
27796438	2	4	used	used	304:307	arg2	CST					256:258	CST	256:258	CST	256:258	The capillary suction time (CST) and water content (WC) of sludge cake were used to evaluate sludge dewaterability.
27796438	2	4	used	used	304:307	arg2	time					250:253	The capillary suction time	228:253	The capillary suction time (CST)	228:259	The capillary suction time (CST) and water content (WC) of sludge cake were used to evaluate sludge dewaterability.
27796438	2	4	used	used	304:307	arg2	WC					280:281	WC	280:281	WC	280:281	The capillary suction time (CST) and water content (WC) of sludge cake were used to evaluate sludge dewaterability.
27796438	2	4	used	used	304:307	arg2	content					271:277	water content	265:277	water content (WC)	265:282	The capillary suction time (CST) and water content (WC) of sludge cake were used to evaluate sludge dewaterability.
27796438	1	5	theme	benzyl	157:162	arg1	DDBAC					183:187	DDBAC	183:187	DDBAC	183:187	The effect of Fenton's reagent combined with dodecyl dimethyl benzyl ammonium chloride (DDBAC) on sludge dewaterability was studied.
27796438	1	5	theme	benzyl	157:162	arg1	chloride					173:180	dodecyl dimethyl benzyl ammonium chloride	140:180	dodecyl dimethyl benzyl ammonium chloride (DDBAC)	140:188	The effect of Fenton's reagent combined with dodecyl dimethyl benzyl ammonium chloride (DDBAC) on sludge dewaterability was studied.
27796438	3	6	from	concentration	443:455	arg1	supernatant					471:481	the sludge supernatant	460:481	the sludge supernatant	460:481	Bound water content (W B), extracellular polymeric substance (EPS) concentration, and organic acid concentration in the sludge supernatant were measured to explain the change of dewaterability in the conditioning process.
27796438	2	7	theme	water	265:269	arg1	WC					280:281	WC	280:281	WC	280:281	The capillary suction time (CST) and water content (WC) of sludge cake were used to evaluate sludge dewaterability.
27796438	2	7	theme	water	265:269	arg1	content					271:277	water content	265:277	water content (WC)	265:282	The capillary suction time (CST) and water content (WC) of sludge cake were used to evaluate sludge dewaterability.
27796438	7	8	theme	bound	1028:1032	arg1	water					1034:1038	the bound water	1024:1038	the bound water	1024:1038	And, approximately 68 % of the bound water was released in this process.
27796438	8	9	theme	conditioning	1172:1183	arg1	process					1185:1191	the composite conditioning process	1158:1191	the composite conditioning process	1158:1191	Further studies through high-performance liquid chromatography (HPLC) demonstrated that the composite conditioning process could oxidize and hydrolyze EPS into small organic molecules, resulting in an increase of small-molecule organics in the species and quantities.
27796438	3	10	theme	acid	438:441	arg1	concentration					443:455	organic acid concentration	430:455	organic acid concentration in the sludge supernatant	430:481	Bound water content (W B), extracellular polymeric substance (EPS) concentration, and organic acid concentration in the sludge supernatant were measured to explain the change of dewaterability in the conditioning process.
27796438	5	11	theme	Fe2+	696:699	arg1	dosage					686:691	the dosage	682:691	the dosage of Fe2+, H2O2, and DDBAC	682:716	When the dosage of Fe2+, H2O2, and DDBAC were 40, 40, and 60 mg g-1 at pH 4, WC of 57.17 % and CST of 17.2 s could be achieved.
27796438	5	11	theme	Fe2+	696:699	arg1	40					723:724	40	723:724	40	723:724	When the dosage of Fe2+, H2O2, and DDBAC were 40, 40, and 60 mg g-1 at pH 4, WC of 57.17 % and CST of 17.2 s could be achieved.
27796438	6	12	theme	bound	949:953	arg1	TB-EPS					960:965	TB-EPS	960:965	TB-EPS	960:965	The protein (PN) and polysaccharide (PS) concentrations in each layer of EPS decreased during the composite conditioning, especially in tightly bound EPS (TB-EPS) and slime layer EPS (S-EPS).
27796438	6	12	theme	bound	949:953	arg1	EPS					955:957	tightly bound EPS	941:957	tightly bound EPS (TB-EPS)	941:966	The protein (PN) and polysaccharide (PS) concentrations in each layer of EPS decreased during the composite conditioning, especially in tightly bound EPS (TB-EPS) and slime layer EPS (S-EPS).
27796438	3	13	theme	sludge	464:469	arg1	supernatant					471:481	the sludge supernatant	460:481	the sludge supernatant	460:481	Bound water content (W B), extracellular polymeric substance (EPS) concentration, and organic acid concentration in the sludge supernatant were measured to explain the change of dewaterability in the conditioning process.
27796438	8	14	theme	Further	1070:1076	arg1	studies					1078:1084	Further studies	1070:1084	Further studies through high-performance liquid chromatography (HPLC)	1070:1138	Further studies through high-performance liquid chromatography (HPLC) demonstrated that the composite conditioning process could oxidize and hydrolyze EPS into small organic molecules, resulting in an increase of small-molecule organics in the species and quantities.
27796438	1	15	theme	ammonium	164:171	arg1	DDBAC					183:187	DDBAC	183:187	DDBAC	183:187	The effect of Fenton's reagent combined with dodecyl dimethyl benzyl ammonium chloride (DDBAC) on sludge dewaterability was studied.
27796438	1	15	theme	ammonium	164:171	arg1	chloride					173:180	dodecyl dimethyl benzyl ammonium chloride	140:180	dodecyl dimethyl benzyl ammonium chloride (DDBAC)	140:188	The effect of Fenton's reagent combined with dodecyl dimethyl benzyl ammonium chloride (DDBAC) on sludge dewaterability was studied.
27796438	6	16	theme	slime	972:976	arg1	S-EPS					989:993	S-EPS	989:993	S-EPS	989:993	The protein (PN) and polysaccharide (PS) concentrations in each layer of EPS decreased during the composite conditioning, especially in tightly bound EPS (TB-EPS) and slime layer EPS (S-EPS).
27796438	6	16	theme	slime	972:976	arg1	EPS					984:986	slime layer EPS	972:986	slime layer EPS (S-EPS)	972:994	The protein (PN) and polysaccharide (PS) concentrations in each layer of EPS decreased during the composite conditioning, especially in tightly bound EPS (TB-EPS) and slime layer EPS (S-EPS).
27796438	3	17	from	concentration	411:423	arg1	supernatant					471:481	the sludge supernatant	460:481	the sludge supernatant	460:481	Bound water content (W B), extracellular polymeric substance (EPS) concentration, and organic acid concentration in the sludge supernatant were measured to explain the change of dewaterability in the conditioning process.
27796438	3	18	theme	polymeric	385:393	arg1	EPS					406:408	EPS	406:408	EPS	406:408	Bound water content (W B), extracellular polymeric substance (EPS) concentration, and organic acid concentration in the sludge supernatant were measured to explain the change of dewaterability in the conditioning process.
27796438	3	18	theme	polymeric	385:393	arg1	substance					395:403	polymeric substance	385:403	extracellular polymeric substance (EPS) concentration	371:423	Bound water content (W B), extracellular polymeric substance (EPS) concentration, and organic acid concentration in the sludge supernatant were measured to explain the change of dewaterability in the conditioning process.
27796438	0	19	theme	sludge	10:15	arg1	dewaterability					17:30	sludge dewaterability	10:30	sludge dewaterability	10:30	Improving sludge dewaterability by combined conditioning with Fenton's reagent and surfactant.
27796438	6	20	theme	polysaccharide	826:839	arg1	concentrations					846:859	The protein (PN) and polysaccharide (PS) concentrations	805:859	concentrations	846:859	The protein (PN) and polysaccharide (PS) concentrations in each layer of EPS decreased during the composite conditioning, especially in tightly bound EPS (TB-EPS) and slime layer EPS (S-EPS).
27796438	6	20	theme	polysaccharide	826:839	arg1	PS					842:843	PS	842:843	PS	842:843	The protein (PN) and polysaccharide (PS) concentrations in each layer of EPS decreased during the composite conditioning, especially in tightly bound EPS (TB-EPS) and slime layer EPS (S-EPS).
27796438	6	21	theme	layer	978:982	arg1	S-EPS					989:993	S-EPS	989:993	S-EPS	989:993	The protein (PN) and polysaccharide (PS) concentrations in each layer of EPS decreased during the composite conditioning, especially in tightly bound EPS (TB-EPS) and slime layer EPS (S-EPS).
27796438	6	21	theme	layer	978:982	arg1	EPS					984:986	slime layer EPS	972:986	slime layer EPS (S-EPS)	972:994	The protein (PN) and polysaccharide (PS) concentrations in each layer of EPS decreased during the composite conditioning, especially in tightly bound EPS (TB-EPS) and slime layer EPS (S-EPS).
27796438	3	22	theme	water	350:354	arg1	content					356:362	Bound water content	344:362	Bound water content (W B)	344:368	Bound water content (W B), extracellular polymeric substance (EPS) concentration, and organic acid concentration in the sludge supernatant were measured to explain the change of dewaterability in the conditioning process.
27796438	3	22	theme	water	350:354	arg1	B					367:367	W B	365:367	W B	365:367	Bound water content (W B), extracellular polymeric substance (EPS) concentration, and organic acid concentration in the sludge supernatant were measured to explain the change of dewaterability in the conditioning process.
27796438	5	23	theme	%	766:766	arg1	WC					754:755	WC	754:755	WC of 57.17 % and CST of 17.2 s	754:784	When the dosage of Fe2+, H2O2, and DDBAC were 40, 40, and 60 mg g-1 at pH 4, WC of 57.17 % and CST of 17.2 s could be achieved.
27796438	8	24	from	increase	1271:1278	arg1	species					1314:1320	the species	1310:1320	the species	1310:1320	Further studies through high-performance liquid chromatography (HPLC) demonstrated that the composite conditioning process could oxidize and hydrolyze EPS into small organic molecules, resulting in an increase of small-molecule organics in the species and quantities.
27796438	8	24	from	increase	1271:1278	arg1	quantities					1326:1335	quantities	1326:1335	quantities	1326:1335	Further studies through high-performance liquid chromatography (HPLC) demonstrated that the composite conditioning process could oxidize and hydrolyze EPS into small organic molecules, resulting in an increase of small-molecule organics in the species and quantities.
27796438	5	25	theme	CST	772:774	arg1	WC					754:755	WC	754:755	WC of 57.17 % and CST of 17.2 s	754:784	When the dosage of Fe2+, H2O2, and DDBAC were 40, 40, and 60 mg g-1 at pH 4, WC of 57.17 % and CST of 17.2 s could be achieved.
27796438	3	26	theme	conditioning	544:555	arg1	process					557:563	the conditioning process	540:563	the conditioning process	540:563	Bound water content (W B), extracellular polymeric substance (EPS) concentration, and organic acid concentration in the sludge supernatant were measured to explain the change of dewaterability in the conditioning process.
27796438	8	27	theme	organics	1298:1305	arg1	increase					1271:1278	an increase	1268:1278	an increase of small-molecule organics in the species and quantities	1268:1335	Further studies through high-performance liquid chromatography (HPLC) demonstrated that the composite conditioning process could oxidize and hydrolyze EPS into small organic molecules, resulting in an increase of small-molecule organics in the species and quantities.
27796438	6	28	theme	composite	903:911	arg1	conditioning					913:924	the composite conditioning	899:924	the composite conditioning	899:924	The protein (PN) and polysaccharide (PS) concentrations in each layer of EPS decreased during the composite conditioning, especially in tightly bound EPS (TB-EPS) and slime layer EPS (S-EPS).
27796438	8	29	theme	small-molecule	1283:1296	arg1	organics					1298:1305	small-molecule organics	1283:1305	small-molecule organics	1283:1305	Further studies through high-performance liquid chromatography (HPLC) demonstrated that the composite conditioning process could oxidize and hydrolyze EPS into small organic molecules, resulting in an increase of small-molecule organics in the species and quantities.
27796438	4	30	theme	sludge	640:645	arg1	dewaterability					647:660	sludge dewaterability	640:660	sludge dewaterability	640:660	Results indicated that Fenton's reagent combined with DDBAC could enhance sludge dewaterability significantly.
27796438	3	31	theme	W	365:365	arg1	content					356:362	Bound water content	344:362	Bound water content (W B)	344:368	Bound water content (W B), extracellular polymeric substance (EPS) concentration, and organic acid concentration in the sludge supernatant were measured to explain the change of dewaterability in the conditioning process.
27796438	3	31	theme	W	365:365	arg1	B					367:367	W B	365:367	W B	365:367	Bound water content (W B), extracellular polymeric substance (EPS) concentration, and organic acid concentration in the sludge supernatant were measured to explain the change of dewaterability in the conditioning process.
27796438	3	32	theme	organic	430:436	arg1	concentration					443:455	organic acid concentration	430:455	organic acid concentration in the sludge supernatant	430:481	Bound water content (W B), extracellular polymeric substance (EPS) concentration, and organic acid concentration in the sludge supernatant were measured to explain the change of dewaterability in the conditioning process.
27796438	1	33	theme	sludge	193:198	arg1	dewaterability					200:213	sludge dewaterability	193:213	sludge dewaterability	193:213	The effect of Fenton's reagent combined with dodecyl dimethyl benzyl ammonium chloride (DDBAC) on sludge dewaterability was studied.
27796438	8	34	theme	organic	1236:1242	arg1	molecules					1244:1252	small organic molecules	1230:1252	small organic molecules	1230:1252	Further studies through high-performance liquid chromatography (HPLC) demonstrated that the composite conditioning process could oxidize and hydrolyze EPS into small organic molecules, resulting in an increase of small-molecule organics in the species and quantities.
27796438	3	35	from	change	512:517	arg1	process					557:563	the conditioning process	540:563	the conditioning process	540:563	Bound water content (W B), extracellular polymeric substance (EPS) concentration, and organic acid concentration in the sludge supernatant were measured to explain the change of dewaterability in the conditioning process.
27796438	3	36	theme	substance	395:403	arg1	concentration					411:423	extracellular polymeric substance (EPS) concentration	371:423	extracellular polymeric substance (EPS) concentration	371:423	Bound water content (W B), extracellular polymeric substance (EPS) concentration, and organic acid concentration in the sludge supernatant were measured to explain the change of dewaterability in the conditioning process.
27796438	0	37	theme	combined	35:42	arg1	conditioning					44:55	combined conditioning	35:55	combined conditioning with Fenton's reagent and surfactant	35:92	Improving sludge dewaterability by combined conditioning with Fenton's reagent and surfactant.
27796438	8	38	theme	liquid	1111:1116	arg1	HPLC					1134:1137	HPLC	1134:1137	HPLC	1134:1137	Further studies through high-performance liquid chromatography (HPLC) demonstrated that the composite conditioning process could oxidize and hydrolyze EPS into small organic molecules, resulting in an increase of small-molecule organics in the species and quantities.
27796438	8	38	theme	liquid	1111:1116	arg1	chromatography					1118:1131	high-performance liquid chromatography	1094:1131	high-performance liquid chromatography (HPLC)	1094:1138	Further studies through high-performance liquid chromatography (HPLC) demonstrated that the composite conditioning process could oxidize and hydrolyze EPS into small organic molecules, resulting in an increase of small-molecule organics in the species and quantities.
27796438	8	39	theme	small	1230:1234	arg1	molecules					1244:1252	small organic molecules	1230:1252	small organic molecules	1230:1252	Further studies through high-performance liquid chromatography (HPLC) demonstrated that the composite conditioning process could oxidize and hydrolyze EPS into small organic molecules, resulting in an increase of small-molecule organics in the species and quantities.
27796438	8	40	theme	high-performance	1094:1109	arg1	HPLC					1134:1137	HPLC	1134:1137	HPLC	1134:1137	Further studies through high-performance liquid chromatography (HPLC) demonstrated that the composite conditioning process could oxidize and hydrolyze EPS into small organic molecules, resulting in an increase of small-molecule organics in the species and quantities.
27796438	8	40	theme	high-performance	1094:1109	arg1	chromatography					1118:1131	high-performance liquid chromatography	1094:1131	high-performance liquid chromatography (HPLC)	1094:1138	Further studies through high-performance liquid chromatography (HPLC) demonstrated that the composite conditioning process could oxidize and hydrolyze EPS into small organic molecules, resulting in an increase of small-molecule organics in the species and quantities.
27796438	3	41	theme	extracellular	371:383	arg1	concentration					411:423	extracellular polymeric substance (EPS) concentration	371:423	extracellular polymeric substance (EPS) concentration	371:423	Bound water content (W B), extracellular polymeric substance (EPS) concentration, and organic acid concentration in the sludge supernatant were measured to explain the change of dewaterability in the conditioning process.
27796438	3	42	theme	dewaterability	522:535	arg1	change					512:517	the change	508:517	the change of dewaterability in the conditioning process	508:563	Bound water content (W B), extracellular polymeric substance (EPS) concentration, and organic acid concentration in the sludge supernatant were measured to explain the change of dewaterability in the conditioning process.
27796438	5	43	from	pH 4	748:751	arg1	dosage					686:691	the dosage	682:691	the dosage of Fe2+, H2O2, and DDBAC	682:716	When the dosage of Fe2+, H2O2, and DDBAC were 40, 40, and 60 mg g-1 at pH 4, WC of 57.17 % and CST of 17.2 s could be achieved.
27796438	5	43	from	pH 4	748:751	arg1	40					723:724	40	723:724	40	723:724	When the dosage of Fe2+, H2O2, and DDBAC were 40, 40, and 60 mg g-1 at pH 4, WC of 57.17 % and CST of 17.2 s could be achieved.
27796438	2	44	theme	cake	294:297	arg1	WC					280:281	WC	280:281	WC	280:281	The capillary suction time (CST) and water content (WC) of sludge cake were used to evaluate sludge dewaterability.
27796438	2	44	theme	cake	294:297	arg1	CST					256:258	CST	256:258	CST	256:258	The capillary suction time (CST) and water content (WC) of sludge cake were used to evaluate sludge dewaterability.
27796438	2	44	theme	cake	294:297	arg1	time					250:253	The capillary suction time	228:253	The capillary suction time (CST)	228:259	The capillary suction time (CST) and water content (WC) of sludge cake were used to evaluate sludge dewaterability.
27796438	2	44	theme	cake	294:297	arg1	content					271:277	water content	265:277	water content (WC)	265:282	The capillary suction time (CST) and water content (WC) of sludge cake were used to evaluate sludge dewaterability.
27796438	1	45	from	effect	99:104	arg1	dewaterability					200:213	sludge dewaterability	193:213	sludge dewaterability	193:213	The effect of Fenton's reagent combined with dodecyl dimethyl benzyl ammonium chloride (DDBAC) on sludge dewaterability was studied.
27796438	1	46	theme	reagent	118:124	arg1	effect					99:104	The effect	95:104	The effect of Fenton's reagent combined with dodecyl dimethyl benzyl ammonium chloride (DDBAC) on sludge dewaterability	95:213	The effect of Fenton's reagent combined with dodecyl dimethyl benzyl ammonium chloride (DDBAC) on sludge dewaterability was studied.
27796438	6	47	from	concentrations	846:859	arg1	layer					869:873	each layer	864:873	each layer of EPS	864:880	The protein (PN) and polysaccharide (PS) concentrations in each layer of EPS decreased during the composite conditioning, especially in tightly bound EPS (TB-EPS) and slime layer EPS (S-EPS).
27796438	5	48	theme	17.2 s	779:784	arg1	%					766:766	57.17 %	760:766	57.17 %	760:766	When the dosage of Fe2+, H2O2, and DDBAC were 40, 40, and 60 mg g-1 at pH 4, WC of 57.17 % and CST of 17.2 s could be achieved.
27796438	5	48	theme	17.2 s	779:784	arg1	17.2 s					779:784	17.2 s	779:784	17.2 s	779:784	When the dosage of Fe2+, H2O2, and DDBAC were 40, 40, and 60 mg g-1 at pH 4, WC of 57.17 % and CST of 17.2 s could be achieved.
27796438	5	48	theme	17.2 s	779:784	arg1	CST					772:774	CST	772:774	CST	772:774	When the dosage of Fe2+, H2O2, and DDBAC were 40, 40, and 60 mg g-1 at pH 4, WC of 57.17 % and CST of 17.2 s could be achieved.
27796438	8	49	theme	composite	1162:1170	arg1	process					1185:1191	the composite conditioning process	1158:1191	the composite conditioning process	1158:1191	Further studies through high-performance liquid chromatography (HPLC) demonstrated that the composite conditioning process could oxidize and hydrolyze EPS into small organic molecules, resulting in an increase of small-molecule organics in the species and quantities.
27796438	2	50	theme	sludge	287:292	arg1	cake					294:297	sludge cake	287:297	sludge cake	287:297	The capillary suction time (CST) and water content (WC) of sludge cake were used to evaluate sludge dewaterability.
27796438	5	51	theme	H2O2	702:705	arg1	dosage					686:691	the dosage	682:691	the dosage of Fe2+, H2O2, and DDBAC	682:716	When the dosage of Fe2+, H2O2, and DDBAC were 40, 40, and 60 mg g-1 at pH 4, WC of 57.17 % and CST of 17.2 s could be achieved.
27796438	5	51	theme	H2O2	702:705	arg1	40					723:724	40	723:724	40	723:724	When the dosage of Fe2+, H2O2, and DDBAC were 40, 40, and 60 mg g-1 at pH 4, WC of 57.17 % and CST of 17.2 s could be achieved.
27796438	2	52	theme	suction	242:248	arg1	CST					256:258	CST	256:258	CST	256:258	The capillary suction time (CST) and water content (WC) of sludge cake were used to evaluate sludge dewaterability.
27796438	2	52	theme	suction	242:248	arg1	time					250:253	The capillary suction time	228:253	The capillary suction time (CST)	228:259	The capillary suction time (CST) and water content (WC) of sludge cake were used to evaluate sludge dewaterability.
27796438	2	53	theme	sludge	321:326	arg1	dewaterability					328:341	sludge dewaterability	321:341	sludge dewaterability	321:341	The capillary suction time (CST) and water content (WC) of sludge cake were used to evaluate sludge dewaterability.
27796438	3	54	theme	Bound	344:348	arg1	content					356:362	Bound water content	344:362	Bound water content (W B)	344:368	Bound water content (W B), extracellular polymeric substance (EPS) concentration, and organic acid concentration in the sludge supernatant were measured to explain the change of dewaterability in the conditioning process.
27796438	3	54	theme	Bound	344:348	arg1	B					367:367	W B	365:367	W B	365:367	Bound water content (W B), extracellular polymeric substance (EPS) concentration, and organic acid concentration in the sludge supernatant were measured to explain the change of dewaterability in the conditioning process.
27796438	6	55	theme	EPS	878:880	arg1	layer					869:873	each layer	864:873	each layer of EPS	864:880	The protein (PN) and polysaccharide (PS) concentrations in each layer of EPS decreased during the composite conditioning, especially in tightly bound EPS (TB-EPS) and slime layer EPS (S-EPS).
27796438	3	56	from	content	356:362	arg1	supernatant					471:481	the sludge supernatant	460:481	the sludge supernatant	460:481	Bound water content (W B), extracellular polymeric substance (EPS) concentration, and organic acid concentration in the sludge supernatant were measured to explain the change of dewaterability in the conditioning process.
27796438	2	57	theme	capillary	232:240	arg1	CST					256:258	CST	256:258	CST	256:258	The capillary suction time (CST) and water content (WC) of sludge cake were used to evaluate sludge dewaterability.
27796438	2	57	theme	capillary	232:240	arg1	time					250:253	The capillary suction time	228:253	The capillary suction time (CST)	228:259	The capillary suction time (CST) and water content (WC) of sludge cake were used to evaluate sludge dewaterability.
27796438	1	58	theme	dodecyl	140:146	arg1	DDBAC					183:187	DDBAC	183:187	DDBAC	183:187	The effect of Fenton's reagent combined with dodecyl dimethyl benzyl ammonium chloride (DDBAC) on sludge dewaterability was studied.
27796438	1	58	theme	dodecyl	140:146	arg1	chloride					173:180	dodecyl dimethyl benzyl ammonium chloride	140:180	dodecyl dimethyl benzyl ammonium chloride (DDBAC)	140:188	The effect of Fenton's reagent combined with dodecyl dimethyl benzyl ammonium chloride (DDBAC) on sludge dewaterability was studied.
27481297	0	0	theme	Ganoderma	49:57	arg1	Agaricomycetes					69:82	Agaricomycetes	69:82	Agaricomycetes	69:82	β-(1→3)-Glucan of the Southern Bracket Mushroom, Ganoderma australe (Agaricomycetes), Stimulates Phagocytosis and Interleukin-6 Production in Mouse Peritoneal Macrophages.
27481297	0	0	theme	Ganoderma	49:57	arg1	Mushroom					39:46	the Southern Bracket Mushroom	18:46	the Southern Bracket Mushroom	18:46	β-(1→3)-Glucan of the Southern Bracket Mushroom, Ganoderma australe (Agaricomycetes), Stimulates Phagocytosis and Interleukin-6 Production in Mouse Peritoneal Macrophages.
27481297	0	0	theme	Ganoderma	49:57	arg1	australe					59:66	Ganoderma australe	49:66	Ganoderma australe (Agaricomycetes)	49:83	β-(1→3)-Glucan of the Southern Bracket Mushroom, Ganoderma australe (Agaricomycetes), Stimulates Phagocytosis and Interleukin-6 Production in Mouse Peritoneal Macrophages.
27481297	1	1	theme	aqueous	319:325	arg1	extraction					350:359	an aqueous 5% potassium hydroxide extraction	316:359	an aqueous 5% potassium hydroxide extraction	316:359	Ganoderma australe was studied to determine the composition of the cell wall, and polysaccharide fraction SK5 was obtained after freeze-thawing an aqueous 5% potassium hydroxide extraction.
27481297	6	2	theme	response	1271:1278	arg1	-glucan					1203:1209	a β-(1→3)-glucan	1194:1209	a β-(1→3)-glucan isolated from G. australe	1194:1235	The results indicate that a β-(1→3)-glucan isolated from G. australe can be classified as a biological response modifier.
27481297	6	2	theme	response	1271:1278	arg1	modifier					1280:1287	a biological response modifier	1258:1287	a biological response modifier	1258:1287	The results indicate that a β-(1→3)-glucan isolated from G. australe can be classified as a biological response modifier.
27481297	3	3	theme	G.	696:697	arg1	australe					699:706	G. australe	696:706	G. australe	696:706	In addition, the biological effect of the β-glucan from G. australe was evaluated via in vitro cell cultures of peritoneal macrophages isolated from Swiss mice.
27481297	1	4	theme	5	327:327	arg1	%					328:328	%	328:328	%	328:328	Ganoderma australe was studied to determine the composition of the cell wall, and polysaccharide fraction SK5 was obtained after freeze-thawing an aqueous 5% potassium hydroxide extraction.
27481297	0	5	theme	Peritoneal	148:157	arg1	Macrophages					159:169	Mouse Peritoneal Macrophages	142:169	Mouse Peritoneal Macrophages	142:169	β-(1→3)-Glucan of the Southern Bracket Mushroom, Ganoderma australe (Agaricomycetes), Stimulates Phagocytosis and Interleukin-6 Production in Mouse Peritoneal Macrophages.
27481297	3	6	theme	biological	657:666	arg1	effect					668:673	the biological effect	653:673	the biological effect of the β-glucan from G. australe	653:706	In addition, the biological effect of the β-glucan from G. australe was evaluated via in vitro cell cultures of peritoneal macrophages isolated from Swiss mice.
27481297	1	7	theme	cell	239:242	arg1	wall					244:247	the cell wall	235:247	the cell wall	235:247	Ganoderma australe was studied to determine the composition of the cell wall, and polysaccharide fraction SK5 was obtained after freeze-thawing an aqueous 5% potassium hydroxide extraction.
27481297	2	8	theme	resonance	521:529	arg1	spectroscopy					531:542	13C nuclear magnetic resonance spectroscopy	500:542	13C nuclear magnetic resonance spectroscopy	500:542	The monosaccharide composition of the SK5 fraction revealed by gas chromatography-mass spectrometry showed 81.3% glucose, and analyses by 13C nuclear magnetic resonance spectroscopy confirmed a β-glucan with glycosidic links of the (1→3)-β type and most likely 4-O substituted.
27481297	1	9	theme	%	328:328	arg1	extraction					350:359	an aqueous 5% potassium hydroxide extraction	316:359	an aqueous 5% potassium hydroxide extraction	316:359	Ganoderma australe was studied to determine the composition of the cell wall, and polysaccharide fraction SK5 was obtained after freeze-thawing an aqueous 5% potassium hydroxide extraction.
27481297	1	10	theme	wall	244:247	arg1	composition					220:230	the composition	216:230	the composition of the cell wall	216:247	Ganoderma australe was studied to determine the composition of the cell wall, and polysaccharide fraction SK5 was obtained after freeze-thawing an aqueous 5% potassium hydroxide extraction.
27481297	2	11	theme	magnetic	512:519	arg1	resonance					521:529	13C nuclear magnetic resonance	500:529	13C nuclear magnetic resonance spectroscopy	500:542	The monosaccharide composition of the SK5 fraction revealed by gas chromatography-mass spectrometry showed 81.3% glucose, and analyses by 13C nuclear magnetic resonance spectroscopy confirmed a β-glucan with glycosidic links of the (1→3)-β type and most likely 4-O substituted.
27481297	1	12	theme	potassium	330:338	arg1	extraction					350:359	an aqueous 5% potassium hydroxide extraction	316:359	an aqueous 5% potassium hydroxide extraction	316:359	Ganoderma australe was studied to determine the composition of the cell wall, and polysaccharide fraction SK5 was obtained after freeze-thawing an aqueous 5% potassium hydroxide extraction.
27481297	4	13	theme	cell	850:853	arg1	activation					855:864	cell activation	850:864	cell activation	850:864	Biological assays were assessed for toxicity and cell activation, interleukin-6 cytokine concentrations, and the ability to stimulate phagocytic activity.
27481297	2	14	theme	4-O	623:625	arg1	links					581:585	glycosidic links	570:585	glycosidic links of the (1→3)-β type and most likely 4-O substituted	570:637	The monosaccharide composition of the SK5 fraction revealed by gas chromatography-mass spectrometry showed 81.3% glucose, and analyses by 13C nuclear magnetic resonance spectroscopy confirmed a β-glucan with glycosidic links of the (1→3)-β type and most likely 4-O substituted.
27481297	6	15	attach	isolated	1211:1218	arg2	-glucan					1203:1209	a β-(1→3)-glucan	1194:1209	a β-(1→3)-glucan isolated from G. australe	1194:1235	The results indicate that a β-(1→3)-glucan isolated from G. australe can be classified as a biological response modifier.
27481297	6	15	attach	isolated	1211:1218	arg2	modifier					1280:1287	a biological response modifier	1258:1287	a biological response modifier	1258:1287	The results indicate that a β-(1→3)-glucan isolated from G. australe can be classified as a biological response modifier.
27481297	6	15	attach	isolated	1211:1218	arg1	australe					1228:1235	G. australe	1225:1235	G. australe	1225:1235	The results indicate that a β-(1→3)-glucan isolated from G. australe can be classified as a biological response modifier.
27481297	2	16	theme	nuclear	504:510	arg1	resonance					521:529	13C nuclear magnetic resonance	500:529	13C nuclear magnetic resonance spectroscopy	500:542	The monosaccharide composition of the SK5 fraction revealed by gas chromatography-mass spectrometry showed 81.3% glucose, and analyses by 13C nuclear magnetic resonance spectroscopy confirmed a β-glucan with glycosidic links of the (1→3)-β type and most likely 4-O substituted.
27481297	1	17	theme	hydroxide	340:348	arg1	extraction					350:359	an aqueous 5% potassium hydroxide extraction	316:359	an aqueous 5% potassium hydroxide extraction	316:359	Ganoderma australe was studied to determine the composition of the cell wall, and polysaccharide fraction SK5 was obtained after freeze-thawing an aqueous 5% potassium hydroxide extraction.
27481297	6	18	theme	G.	1225:1226	arg1	australe					1228:1235	G. australe	1225:1235	G. australe	1225:1235	The results indicate that a β-(1→3)-glucan isolated from G. australe can be classified as a biological response modifier.
27481297	2	19	theme	likely	616:621	arg1	4-O					623:625	most likely 4-O	611:625	most likely 4-O	611:625	The monosaccharide composition of the SK5 fraction revealed by gas chromatography-mass spectrometry showed 81.3% glucose, and analyses by 13C nuclear magnetic resonance spectroscopy confirmed a β-glucan with glycosidic links of the (1→3)-β type and most likely 4-O substituted.
27481297	6	20	theme	biological	1260:1269	arg1	-glucan					1203:1209	a β-(1→3)-glucan	1194:1209	a β-(1→3)-glucan isolated from G. australe	1194:1235	The results indicate that a β-(1→3)-glucan isolated from G. australe can be classified as a biological response modifier.
27481297	6	20	theme	biological	1260:1269	arg1	modifier					1280:1287	a biological response modifier	1258:1287	a biological response modifier	1258:1287	The results indicate that a β-(1→3)-glucan isolated from G. australe can be classified as a biological response modifier.
27481297	3	21	theme	Swiss	789:793	arg1	mice					795:798	Swiss mice	789:798	Swiss mice	789:798	In addition, the biological effect of the β-glucan from G. australe was evaluated via in vitro cell cultures of peritoneal macrophages isolated from Swiss mice.
27481297	2	22	theme	13C	500:502	arg1	resonance					521:529	13C nuclear magnetic resonance	500:529	13C nuclear magnetic resonance spectroscopy	500:542	The monosaccharide composition of the SK5 fraction revealed by gas chromatography-mass spectrometry showed 81.3% glucose, and analyses by 13C nuclear magnetic resonance spectroscopy confirmed a β-glucan with glycosidic links of the (1→3)-β type and most likely 4-O substituted.
27481297	3	23	theme	β-glucan	682:689	arg1	effect					668:673	the biological effect	653:673	the biological effect of the β-glucan from G. australe	653:706	In addition, the biological effect of the β-glucan from G. australe was evaluated via in vitro cell cultures of peritoneal macrophages isolated from Swiss mice.
27481297	3	24	attach	isolated	775:782	arg1	mice					795:798	Swiss mice	789:798	Swiss mice	789:798	In addition, the biological effect of the β-glucan from G. australe was evaluated via in vitro cell cultures of peritoneal macrophages isolated from Swiss mice.
27481297	3	24	attach	isolated	775:782	arg2	macrophages					763:773	peritoneal macrophages	752:773	peritoneal macrophages isolated from Swiss mice	752:798	In addition, the biological effect of the β-glucan from G. australe was evaluated via in vitro cell cultures of peritoneal macrophages isolated from Swiss mice.
27481297	2	25	theme	chromatography-mass	429:447	arg1	spectrometry					449:460	gas chromatography-mass spectrometry	425:460	gas chromatography-mass spectrometry	425:460	The monosaccharide composition of the SK5 fraction revealed by gas chromatography-mass spectrometry showed 81.3% glucose, and analyses by 13C nuclear magnetic resonance spectroscopy confirmed a β-glucan with glycosidic links of the (1→3)-β type and most likely 4-O substituted.
27481297	5	26	from	increase	969:976	arg1	interleukin-6					981:993	interleukin-6	981:993	interleukin-6	981:993	There was an increase in interleukin-6 by approximately 111% with 1.0 µg/mL of polysaccharide, and phagocyte activity was increased in all concentrations examined, obtaining 52.3% with 0.25 µg/mL polysaccharide.
27481297	2	27	dep	type	602:605	arg1	1→3					595:597	1→3	595:597	1→3	595:597	The monosaccharide composition of the SK5 fraction revealed by gas chromatography-mass spectrometry showed 81.3% glucose, and analyses by 13C nuclear magnetic resonance spectroscopy confirmed a β-glucan with glycosidic links of the (1→3)-β type and most likely 4-O substituted.
27481297	2	27	dep	type	602:605	arg1	the					590:592	the	590:592	the	590:592	The monosaccharide composition of the SK5 fraction revealed by gas chromatography-mass spectrometry showed 81.3% glucose, and analyses by 13C nuclear magnetic resonance spectroscopy confirmed a β-glucan with glycosidic links of the (1→3)-β type and most likely 4-O substituted.
27481297	2	28	theme	type	602:605	arg1	links					581:585	glycosidic links	570:585	glycosidic links of the (1→3)-β type and most likely 4-O substituted	570:637	The monosaccharide composition of the SK5 fraction revealed by gas chromatography-mass spectrometry showed 81.3% glucose, and analyses by 13C nuclear magnetic resonance spectroscopy confirmed a β-glucan with glycosidic links of the (1→3)-β type and most likely 4-O substituted.
27481297	5	29	theme	0.25	1141:1144	arg1	µg/mL					1146:1150	µg/mL	1146:1150	µg/mL	1146:1150	There was an increase in interleukin-6 by approximately 111% with 1.0 µg/mL of polysaccharide, and phagocyte activity was increased in all concentrations examined, obtaining 52.3% with 0.25 µg/mL polysaccharide.
27481297	2	30	theme	monosaccharide	366:379	arg1	composition					381:391	The monosaccharide composition	362:391	The monosaccharide composition of the SK5 fraction revealed by gas chromatography-mass spectrometry	362:460	The monosaccharide composition of the SK5 fraction revealed by gas chromatography-mass spectrometry showed 81.3% glucose, and analyses by 13C nuclear magnetic resonance spectroscopy confirmed a β-glucan with glycosidic links of the (1→3)-β type and most likely 4-O substituted.
27481297	0	31	theme	β-	0:1	arg1	-Glucan					7:13	β-(1→3)-Glucan	0:13	β-(1→3)-Glucan of the Southern Bracket Mushroom, Ganoderma australe (Agaricomycetes),	0:84	β-(1→3)-Glucan of the Southern Bracket Mushroom, Ganoderma australe (Agaricomycetes), Stimulates Phagocytosis and Interleukin-6 Production in Mouse Peritoneal Macrophages.
27481297	5	32	theme	µg/mL	1146:1150	arg1	polysaccharide					1152:1165	0.25 µg/mL polysaccharide	1141:1165	0.25 µg/mL polysaccharide	1141:1165	There was an increase in interleukin-6 by approximately 111% with 1.0 µg/mL of polysaccharide, and phagocyte activity was increased in all concentrations examined, obtaining 52.3% with 0.25 µg/mL polysaccharide.
27481297	2	33	theme	-β	599:600	arg1	type					602:605	(1→3)-β type	594:605	(1→3)-β type	594:605	The monosaccharide composition of the SK5 fraction revealed by gas chromatography-mass spectrometry showed 81.3% glucose, and analyses by 13C nuclear magnetic resonance spectroscopy confirmed a β-glucan with glycosidic links of the (1→3)-β type and most likely 4-O substituted.
27481297	1	34	theme	Ganoderma	172:180	arg1	australe					182:189	Ganoderma australe	172:189	Ganoderma australe	172:189	Ganoderma australe was studied to determine the composition of the cell wall, and polysaccharide fraction SK5 was obtained after freeze-thawing an aqueous 5% potassium hydroxide extraction.
27481297	2	35	theme	gas	425:427	arg1	spectrometry					449:460	gas chromatography-mass spectrometry	425:460	gas chromatography-mass spectrometry	425:460	The monosaccharide composition of the SK5 fraction revealed by gas chromatography-mass spectrometry showed 81.3% glucose, and analyses by 13C nuclear magnetic resonance spectroscopy confirmed a β-glucan with glycosidic links of the (1→3)-β type and most likely 4-O substituted.
27481297	1	36	theme	polysaccharide	254:267	arg1	fraction					269:276	polysaccharide fraction SK5	254:280	polysaccharide fraction SK5	254:280	Ganoderma australe was studied to determine the composition of the cell wall, and polysaccharide fraction SK5 was obtained after freeze-thawing an aqueous 5% potassium hydroxide extraction.
27481297	5	37	with	%	1134:1134	arg1	polysaccharide					1152:1165	0.25 µg/mL polysaccharide	1141:1165	0.25 µg/mL polysaccharide	1141:1165	There was an increase in interleukin-6 by approximately 111% with 1.0 µg/mL of polysaccharide, and phagocyte activity was increased in all concentrations examined, obtaining 52.3% with 0.25 µg/mL polysaccharide.
27481297	0	38	dep	Mushroom	39:46	arg1	Bracket					31:37	Bracket	31:37	Bracket	31:37	β-(1→3)-Glucan of the Southern Bracket Mushroom, Ganoderma australe (Agaricomycetes), Stimulates Phagocytosis and Interleukin-6 Production in Mouse Peritoneal Macrophages.
27481297	0	39	theme	1→3	3:5	arg1	-Glucan					7:13	β-(1→3)-Glucan	0:13	β-(1→3)-Glucan of the Southern Bracket Mushroom, Ganoderma australe (Agaricomycetes),	0:84	β-(1→3)-Glucan of the Southern Bracket Mushroom, Ganoderma australe (Agaricomycetes), Stimulates Phagocytosis and Interleukin-6 Production in Mouse Peritoneal Macrophages.
27481297	2	40	theme	81.3	469:472	arg1	%					473:473	%	473:473	%	473:473	The monosaccharide composition of the SK5 fraction revealed by gas chromatography-mass spectrometry showed 81.3% glucose, and analyses by 13C nuclear magnetic resonance spectroscopy confirmed a β-glucan with glycosidic links of the (1→3)-β type and most likely 4-O substituted.
27481297	2	41	theme	fraction	404:411	arg1	composition					381:391	The monosaccharide composition	362:391	The monosaccharide composition of the SK5 fraction revealed by gas chromatography-mass spectrometry	362:460	The monosaccharide composition of the SK5 fraction revealed by gas chromatography-mass spectrometry showed 81.3% glucose, and analyses by 13C nuclear magnetic resonance spectroscopy confirmed a β-glucan with glycosidic links of the (1→3)-β type and most likely 4-O substituted.
27481297	2	42	theme	SK5	400:402	arg1	fraction					404:411	the SK5 fraction	396:411	the SK5 fraction revealed by gas chromatography-mass spectrometry	396:460	The monosaccharide composition of the SK5 fraction revealed by gas chromatography-mass spectrometry showed 81.3% glucose, and analyses by 13C nuclear magnetic resonance spectroscopy confirmed a β-glucan with glycosidic links of the (1→3)-β type and most likely 4-O substituted.
27481297	2	43	theme	%	473:473	arg1	glucose					475:481	81.3% glucose	469:481	81.3% glucose	469:481	The monosaccharide composition of the SK5 fraction revealed by gas chromatography-mass spectrometry showed 81.3% glucose, and analyses by 13C nuclear magnetic resonance spectroscopy confirmed a β-glucan with glycosidic links of the (1→3)-β type and most likely 4-O substituted.
27481297	5	44	theme	phagocyte	1055:1063	arg1	activity					1065:1072	phagocyte activity	1055:1072	phagocyte activity	1055:1072	There was an increase in interleukin-6 by approximately 111% with 1.0 µg/mL of polysaccharide, and phagocyte activity was increased in all concentrations examined, obtaining 52.3% with 0.25 µg/mL polysaccharide.
27481297	6	45	theme	1→3	1199:1201	arg1	-glucan					1203:1209	a β-(1→3)-glucan	1194:1209	a β-(1→3)-glucan isolated from G. australe	1194:1235	The results indicate that a β-(1→3)-glucan isolated from G. australe can be classified as a biological response modifier.
27481297	6	45	theme	1→3	1199:1201	arg1	modifier					1280:1287	a biological response modifier	1258:1287	a biological response modifier	1258:1287	The results indicate that a β-(1→3)-glucan isolated from G. australe can be classified as a biological response modifier.
27481297	4	46	theme	phagocytic	935:944	arg1	activity					946:953	phagocytic activity	935:953	phagocytic activity	935:953	Biological assays were assessed for toxicity and cell activation, interleukin-6 cytokine concentrations, and the ability to stimulate phagocytic activity.
27481297	0	47	theme	Southern	22:29	arg1	Mushroom					39:46	the Southern Bracket Mushroom	18:46	the Southern Bracket Mushroom	18:46	β-(1→3)-Glucan of the Southern Bracket Mushroom, Ganoderma australe (Agaricomycetes), Stimulates Phagocytosis and Interleukin-6 Production in Mouse Peritoneal Macrophages.
27481297	0	47	theme	Southern	22:29	arg1	australe					59:66	Ganoderma australe	49:66	Ganoderma australe (Agaricomycetes)	49:83	β-(1→3)-Glucan of the Southern Bracket Mushroom, Ganoderma australe (Agaricomycetes), Stimulates Phagocytosis and Interleukin-6 Production in Mouse Peritoneal Macrophages.
27481297	5	48	with	%	1015:1015	arg1	µg/mL					1026:1030	1.0 µg/mL	1022:1030	1.0 µg/mL of polysaccharide	1022:1048	There was an increase in interleukin-6 by approximately 111% with 1.0 µg/mL of polysaccharide, and phagocyte activity was increased in all concentrations examined, obtaining 52.3% with 0.25 µg/mL polysaccharide.
27481297	4	49	theme	interleukin-6	867:879	arg1	concentrations					890:903	interleukin-6 cytokine concentrations	867:903	interleukin-6 cytokine concentrations	867:903	Biological assays were assessed for toxicity and cell activation, interleukin-6 cytokine concentrations, and the ability to stimulate phagocytic activity.
27481297	3	50	theme	in	726:727	arg1	cultures					740:747	in vitro cell cultures	726:747	in vitro cell cultures of peritoneal macrophages isolated from Swiss mice	726:798	In addition, the biological effect of the β-glucan from G. australe was evaluated via in vitro cell cultures of peritoneal macrophages isolated from Swiss mice.
27481297	4	51	theme	Biological	801:810	arg1	assays					812:817	Biological assays	801:817	Biological assays	801:817	Biological assays were assessed for toxicity and cell activation, interleukin-6 cytokine concentrations, and the ability to stimulate phagocytic activity.
27481297	2	52	theme	glycosidic	570:579	arg1	links					581:585	glycosidic links	570:585	glycosidic links of the (1→3)-β type and most likely 4-O substituted	570:637	The monosaccharide composition of the SK5 fraction revealed by gas chromatography-mass spectrometry showed 81.3% glucose, and analyses by 13C nuclear magnetic resonance spectroscopy confirmed a β-glucan with glycosidic links of the (1→3)-β type and most likely 4-O substituted.
27481297	3	53	dep	in	726:727	arg1	vitro					729:733	vitro	729:733	vitro	729:733	In addition, the biological effect of the β-glucan from G. australe was evaluated via in vitro cell cultures of peritoneal macrophages isolated from Swiss mice.
27481297	0	54	theme	Interleukin-6	114:126	arg1	Production					128:137	Interleukin-6 Production	114:137	Interleukin-6 Production	114:137	β-(1→3)-Glucan of the Southern Bracket Mushroom, Ganoderma australe (Agaricomycetes), Stimulates Phagocytosis and Interleukin-6 Production in Mouse Peritoneal Macrophages.
27481297	6	55	theme	β-	1196:1197	arg1	-glucan					1203:1209	a β-(1→3)-glucan	1194:1209	a β-(1→3)-glucan isolated from G. australe	1194:1235	The results indicate that a β-(1→3)-glucan isolated from G. australe can be classified as a biological response modifier.
27481297	6	55	theme	β-	1196:1197	arg1	modifier					1280:1287	a biological response modifier	1258:1287	a biological response modifier	1258:1287	The results indicate that a β-(1→3)-glucan isolated from G. australe can be classified as a biological response modifier.
27481297	3	56	theme	cell	735:738	arg1	cultures					740:747	in vitro cell cultures	726:747	in vitro cell cultures of peritoneal macrophages isolated from Swiss mice	726:798	In addition, the biological effect of the β-glucan from G. australe was evaluated via in vitro cell cultures of peritoneal macrophages isolated from Swiss mice.
27481297	0	57	theme	Mouse	142:146	arg1	Macrophages					159:169	Mouse Peritoneal Macrophages	142:169	Mouse Peritoneal Macrophages	142:169	β-(1→3)-Glucan of the Southern Bracket Mushroom, Ganoderma australe (Agaricomycetes), Stimulates Phagocytosis and Interleukin-6 Production in Mouse Peritoneal Macrophages.
27481297	4	58	theme	cytokine	881:888	arg1	concentrations					890:903	interleukin-6 cytokine concentrations	867:903	interleukin-6 cytokine concentrations	867:903	Biological assays were assessed for toxicity and cell activation, interleukin-6 cytokine concentrations, and the ability to stimulate phagocytic activity.
27481297	2	59	with	β-glucan	556:563	arg1	links					581:585	glycosidic links	570:585	glycosidic links of the (1→3)-β type and most likely 4-O substituted	570:637	The monosaccharide composition of the SK5 fraction revealed by gas chromatography-mass spectrometry showed 81.3% glucose, and analyses by 13C nuclear magnetic resonance spectroscopy confirmed a β-glucan with glycosidic links of the (1→3)-β type and most likely 4-O substituted.
27481297	0	60	from	Phagocytosis	97:108	arg1	Macrophages					159:169	Mouse Peritoneal Macrophages	142:169	Mouse Peritoneal Macrophages	142:169	β-(1→3)-Glucan of the Southern Bracket Mushroom, Ganoderma australe (Agaricomycetes), Stimulates Phagocytosis and Interleukin-6 Production in Mouse Peritoneal Macrophages.
27481297	0	61	theme	Mushroom	39:46	arg1	-Glucan					7:13	β-(1→3)-Glucan	0:13	β-(1→3)-Glucan of the Southern Bracket Mushroom, Ganoderma australe (Agaricomycetes),	0:84	β-(1→3)-Glucan of the Southern Bracket Mushroom, Ganoderma australe (Agaricomycetes), Stimulates Phagocytosis and Interleukin-6 Production in Mouse Peritoneal Macrophages.
27481297	5	62	theme	polysaccharide	1035:1048	arg1	µg/mL					1026:1030	1.0 µg/mL	1022:1030	1.0 µg/mL of polysaccharide	1022:1048	There was an increase in interleukin-6 by approximately 111% with 1.0 µg/mL of polysaccharide, and phagocyte activity was increased in all concentrations examined, obtaining 52.3% with 0.25 µg/mL polysaccharide.
27481297	3	63	theme	peritoneal	752:761	arg1	macrophages					763:773	peritoneal macrophages	752:773	peritoneal macrophages isolated from Swiss mice	752:798	In addition, the biological effect of the β-glucan from G. australe was evaluated via in vitro cell cultures of peritoneal macrophages isolated from Swiss mice.
27481297	0	64	from	Production	128:137	arg1	Macrophages					159:169	Mouse Peritoneal Macrophages	142:169	Mouse Peritoneal Macrophages	142:169	β-(1→3)-Glucan of the Southern Bracket Mushroom, Ganoderma australe (Agaricomycetes), Stimulates Phagocytosis and Interleukin-6 Production in Mouse Peritoneal Macrophages.
27481297	3	65	from	australe	699:706	arg1	effect					668:673	the biological effect	653:673	the biological effect of the β-glucan from G. australe	653:706	In addition, the biological effect of the β-glucan from G. australe was evaluated via in vitro cell cultures of peritoneal macrophages isolated from Swiss mice.
27481297	3	66	theme	macrophages	763:773	arg1	cultures					740:747	in vitro cell cultures	726:747	in vitro cell cultures of peritoneal macrophages isolated from Swiss mice	726:798	In addition, the biological effect of the β-glucan from G. australe was evaluated via in vitro cell cultures of peritoneal macrophages isolated from Swiss mice.
27275603	7	0	theme	concentration	1166:1178	arg1	dependence					1180:1189	concentration dependence	1166:1189	concentration dependence	1166:1189	In this study, the influence of sucrose on bacterial adhesion, biofilm composition (dry weight, bacterial counts, and water-insoluble extracellular polysaccharide (EPS) content), and acidogenicity followed a 2nd order polynomial curve with concentration dependence, and the maximum effective concentrations (MECs) of sucrose ranged from 0.45 to 2.4%.
27275603	6	1	theme	polynomial	886:895	arg1	fitting					903:909	2nd order polynomial curve fitting	876:909	2nd order polynomial curve fitting	876:909	To determine the relationships, 2nd order polynomial curve fitting was performed.
27275603	7	2	theme	EPS	1090:1092	arg1	content					1095:1101	water-insoluble extracellular polysaccharide (EPS) content	1044:1101	water-insoluble extracellular polysaccharide (EPS) content	1044:1101	In this study, the influence of sucrose on bacterial adhesion, biofilm composition (dry weight, bacterial counts, and water-insoluble extracellular polysaccharide (EPS) content), and acidogenicity followed a 2nd order polynomial curve with concentration dependence, and the maximum effective concentrations (MECs) of sucrose ranged from 0.45 to 2.4%.
27275603	11	3	theme	oral	1800:1803	arg1	cavity					1805:1810	the oral cavity	1796:1810	the oral cavity	1796:1810	These results suggest that the relationship between sucrose concentration and cariogenic biofilm formation in the oral cavity could be described by a functional relationship.
27275603	4	4	from	discs	511:515	arg1	medium					528:533	culture medium	520:533	culture medium containing 0, 0.05, 0.1, 0.5, 1, 2, 5, 10, 20, or 40% (w/v) sucrose	520:601	S. mutans biofilms were formed on saliva-coated hydroxyapatite discs in culture medium containing 0, 0.05, 0.1, 0.5, 1, 2, 5, 10, 20, or 40% (w/v) sucrose.
27275603	5	5	theme	microbiological	721:735	arg1	methods					835:841	microbiological, biochemical, laser scanning confocal fluorescence microscopic, and scanning electron microscopic methods	721:841	microbiological, biochemical, laser scanning confocal fluorescence microscopic, and scanning electron microscopic methods	721:841	The adherence (in 4-hour biofilms) and biofilm composition (in 46-hour biofilms) of the biofilms were analyzed using microbiological, biochemical, laser scanning confocal fluorescence microscopic, and scanning electron microscopic methods.
27275603	6	6	theme	order	880:884	arg1	fitting					903:909	2nd order polynomial curve fitting	876:909	2nd order polynomial curve fitting	876:909	To determine the relationships, 2nd order polynomial curve fitting was performed.
27275603	4	7	dep	discs	511:515	arg1	biofilms					458:465	biofilms	458:465	biofilms	458:465	S. mutans biofilms were formed on saliva-coated hydroxyapatite discs in culture medium containing 0, 0.05, 0.1, 0.5, 1, 2, 5, 10, 20, or 40% (w/v) sucrose.
27275603	4	7	dep	discs	511:515	arg1	on					479:480	on	479:480	on	479:480	S. mutans biofilms were formed on saliva-coated hydroxyapatite discs in culture medium containing 0, 0.05, 0.1, 0.5, 1, 2, 5, 10, 20, or 40% (w/v) sucrose.
27275603	4	7	dep	discs	511:515	arg1	were					467:470	were	467:470	were	467:470	S. mutans biofilms were formed on saliva-coated hydroxyapatite discs in culture medium containing 0, 0.05, 0.1, 0.5, 1, 2, 5, 10, 20, or 40% (w/v) sucrose.
27275603	4	7	dep	discs	511:515	arg1	mutans					451:456	S. mutans	448:456	S. mutans	448:456	S. mutans biofilms were formed on saliva-coated hydroxyapatite discs in culture medium containing 0, 0.05, 0.1, 0.5, 1, 2, 5, 10, 20, or 40% (w/v) sucrose.
27275603	4	7	dep	discs	511:515	arg1	formed					472:477	formed	472:477	formed	472:477	S. mutans biofilms were formed on saliva-coated hydroxyapatite discs in culture medium containing 0, 0.05, 0.1, 0.5, 1, 2, 5, 10, 20, or 40% (w/v) sucrose.
27275603	7	8	theme	maximum	1200:1206	arg1	MECs					1234:1237	MECs	1234:1237	MECs	1234:1237	In this study, the influence of sucrose on bacterial adhesion, biofilm composition (dry weight, bacterial counts, and water-insoluble extracellular polysaccharide (EPS) content), and acidogenicity followed a 2nd order polynomial curve with concentration dependence, and the maximum effective concentrations (MECs) of sucrose ranged from 0.45 to 2.4%.
27275603	7	8	theme	maximum	1200:1206	arg1	concentrations					1218:1231	the maximum effective concentrations	1196:1231	the maximum effective concentrations (MECs) of sucrose	1196:1249	In this study, the influence of sucrose on bacterial adhesion, biofilm composition (dry weight, bacterial counts, and water-insoluble extracellular polysaccharide (EPS) content), and acidogenicity followed a 2nd order polynomial curve with concentration dependence, and the maximum effective concentrations (MECs) of sucrose ranged from 0.45 to 2.4%.
27275603	8	9	theme	sucrose	1387:1393	arg1	concentration					1395:1407	sucrose concentration	1387:1407	sucrose concentration	1387:1407	The bacterial and EPS bio-volume and thickness in the biofilms also gradually increased and then decreased as sucrose concentration increased.
27275603	6	10	theme	2nd	876:878	arg1	fitting					903:909	2nd order polynomial curve fitting	876:909	2nd order polynomial curve fitting	876:909	To determine the relationships, 2nd order polynomial curve fitting was performed.
27275603	5	11	theme	4-hour	622:627	arg1	biofilms					629:636	4-hour biofilms	622:636	4-hour biofilms	622:636	The adherence (in 4-hour biofilms) and biofilm composition (in 46-hour biofilms) of the biofilms were analyzed using microbiological, biochemical, laser scanning confocal fluorescence microscopic, and scanning electron microscopic methods.
27275603	11	12	theme	functional	1836:1845	arg1	relationship					1847:1858	a functional relationship	1834:1858	a functional relationship	1834:1858	These results suggest that the relationship between sucrose concentration and cariogenic biofilm formation in the oral cavity could be described by a functional relationship.
27275603	7	13	dep	2.4	1271:1273	arg1	to					1268:1269	to	1268:1269	to	1268:1269	In this study, the influence of sucrose on bacterial adhesion, biofilm composition (dry weight, bacterial counts, and water-insoluble extracellular polysaccharide (EPS) content), and acidogenicity followed a 2nd order polynomial curve with concentration dependence, and the maximum effective concentrations (MECs) of sucrose ranged from 0.45 to 2.4%.
27275603	8	14	from	bio-volume	1299:1308	arg1	biofilms					1331:1338	the biofilms	1327:1338	the biofilms	1327:1338	The bacterial and EPS bio-volume and thickness in the biofilms also gradually increased and then decreased as sucrose concentration increased.
27275603	7	15	theme	polynomial	1144:1153	arg1	curve					1155:1159	a 2nd order polynomial curve	1132:1159	a 2nd order polynomial curve with concentration dependence	1132:1189	In this study, the influence of sucrose on bacterial adhesion, biofilm composition (dry weight, bacterial counts, and water-insoluble extracellular polysaccharide (EPS) content), and acidogenicity followed a 2nd order polynomial curve with concentration dependence, and the maximum effective concentrations (MECs) of sucrose ranged from 0.45 to 2.4%.
27275603	7	16	theme	order	1138:1142	arg1	curve					1155:1159	a 2nd order polynomial curve	1132:1159	a 2nd order polynomial curve with concentration dependence	1132:1189	In this study, the influence of sucrose on bacterial adhesion, biofilm composition (dry weight, bacterial counts, and water-insoluble extracellular polysaccharide (EPS) content), and acidogenicity followed a 2nd order polynomial curve with concentration dependence, and the maximum effective concentrations (MECs) of sucrose ranged from 0.45 to 2.4%.
27275603	7	17	theme	water-insoluble	1044:1058	arg1	content					1095:1101	water-insoluble extracellular polysaccharide (EPS) content	1044:1101	water-insoluble extracellular polysaccharide (EPS) content	1044:1101	In this study, the influence of sucrose on bacterial adhesion, biofilm composition (dry weight, bacterial counts, and water-insoluble extracellular polysaccharide (EPS) content), and acidogenicity followed a 2nd order polynomial curve with concentration dependence, and the maximum effective concentrations (MECs) of sucrose ranged from 0.45 to 2.4%.
27275603	8	18	from	thickness	1314:1322	arg1	biofilms					1331:1338	the biofilms	1327:1338	the biofilms	1327:1338	The bacterial and EPS bio-volume and thickness in the biofilms also gradually increased and then decreased as sucrose concentration increased.
27275603	2	19	theme	biofilm	313:319	arg1	formation					321:329	biofilm formation	313:329	biofilm formation	313:329	This study investigated the functional relationships between sucrose concentration and Streptococcus mutans adherence and biofilm formation.
27275603	1	20	theme	dental	176:181	arg1	caries					183:188	dental caries	176:188	dental caries	176:188	Sucrose is an important dietary factor in cariogenic biofilm formation and subsequent initiation of dental caries.
27275603	3	21	theme	sucrose	405:411	arg1	concentration					413:425	sucrose concentration	405:425	sucrose concentration	405:425	Changes in morphological characteristics of the biofilms with increasing sucrose concentration were also evaluated.
27275603	7	22	theme	extracellular	1060:1072	arg1	content					1095:1101	water-insoluble extracellular polysaccharide (EPS) content	1044:1101	water-insoluble extracellular polysaccharide (EPS) content	1044:1101	In this study, the influence of sucrose on bacterial adhesion, biofilm composition (dry weight, bacterial counts, and water-insoluble extracellular polysaccharide (EPS) content), and acidogenicity followed a 2nd order polynomial curve with concentration dependence, and the maximum effective concentrations (MECs) of sucrose ranged from 0.45 to 2.4%.
27275603	5	23	from	composition	651:661	arg1	biofilms					675:682	46-hour biofilms	667:682	46-hour biofilms	667:682	The adherence (in 4-hour biofilms) and biofilm composition (in 46-hour biofilms) of the biofilms were analyzed using microbiological, biochemical, laser scanning confocal fluorescence microscopic, and scanning electron microscopic methods.
27275603	5	23	from	composition	651:661	arg1	biofilms					629:636	4-hour biofilms	622:636	4-hour biofilms	622:636	The adherence (in 4-hour biofilms) and biofilm composition (in 46-hour biofilms) of the biofilms were analyzed using microbiological, biochemical, laser scanning confocal fluorescence microscopic, and scanning electron microscopic methods.
27275603	1	24	theme	caries	183:188	arg1	formation					137:145	cariogenic biofilm formation	118:145	cariogenic biofilm formation	118:145	Sucrose is an important dietary factor in cariogenic biofilm formation and subsequent initiation of dental caries.
27275603	1	24	theme	caries	183:188	arg1	initiation					162:171	subsequent initiation	151:171	subsequent initiation	151:171	Sucrose is an important dietary factor in cariogenic biofilm formation and subsequent initiation of dental caries.
27275603	11	25	theme	biofilm	1775:1781	arg1	formation					1783:1791	cariogenic biofilm formation	1764:1791	cariogenic biofilm formation	1764:1791	These results suggest that the relationship between sucrose concentration and cariogenic biofilm formation in the oral cavity could be described by a functional relationship.
27275603	9	26	theme	sucrose	1506:1512	arg1	concentration					1514:1526	the sucrose concentration	1502:1526	the sucrose concentration	1502:1526	Furthermore, the size and shape of the micro-colonies of the biofilms depended on the sucrose concentration.
27275603	0	27	theme	Functional	0:9	arg1	Relationship					11:22	Functional Relationship	0:22	Functional Relationship between Sucrose and a Cariogenic Biofilm Formation.	0:74	Functional Relationship between Sucrose and a Cariogenic Biofilm Formation.
27275603	4	28	theme	saliva-coated	482:494	arg1	discs					511:515	saliva-coated hydroxyapatite discs	482:515	saliva-coated hydroxyapatite discs in culture medium containing 0, 0.05, 0.1, 0.5, 1, 2, 5, 10, 20, or 40% (w/v) sucrose	482:601	S. mutans biofilms were formed on saliva-coated hydroxyapatite discs in culture medium containing 0, 0.05, 0.1, 0.5, 1, 2, 5, 10, 20, or 40% (w/v) sucrose.
27275603	1	29	theme	important	90:98	arg1	Sucrose					76:82	Sucrose	76:82	Sucrose	76:82	Sucrose is an important dietary factor in cariogenic biofilm formation and subsequent initiation of dental caries.
27275603	1	29	theme	important	90:98	arg1	factor					108:113	an important dietary factor	87:113	an important dietary factor in cariogenic biofilm formation and subsequent initiation of dental caries	87:188	Sucrose is an important dietary factor in cariogenic biofilm formation and subsequent initiation of dental caries.
27275603	5	30	from	adherence	608:616	arg1	biofilms					675:682	46-hour biofilms	667:682	46-hour biofilms	667:682	The adherence (in 4-hour biofilms) and biofilm composition (in 46-hour biofilms) of the biofilms were analyzed using microbiological, biochemical, laser scanning confocal fluorescence microscopic, and scanning electron microscopic methods.
27275603	5	30	from	adherence	608:616	arg1	biofilms					629:636	4-hour biofilms	622:636	4-hour biofilms	622:636	The adherence (in 4-hour biofilms) and biofilm composition (in 46-hour biofilms) of the biofilms were analyzed using microbiological, biochemical, laser scanning confocal fluorescence microscopic, and scanning electron microscopic methods.
27275603	7	31	theme	bacterial	1022:1030	arg1	counts					1032:1037	bacterial counts	1022:1037	bacterial counts	1022:1037	In this study, the influence of sucrose on bacterial adhesion, biofilm composition (dry weight, bacterial counts, and water-insoluble extracellular polysaccharide (EPS) content), and acidogenicity followed a 2nd order polynomial curve with concentration dependence, and the maximum effective concentrations (MECs) of sucrose ranged from 0.45 to 2.4%.
27275603	1	32	theme	dietary	100:106	arg1	Sucrose					76:82	Sucrose	76:82	Sucrose	76:82	Sucrose is an important dietary factor in cariogenic biofilm formation and subsequent initiation of dental caries.
27275603	1	32	theme	dietary	100:106	arg1	factor					108:113	an important dietary factor	87:113	an important dietary factor in cariogenic biofilm formation and subsequent initiation of dental caries	87:188	Sucrose is an important dietary factor in cariogenic biofilm formation and subsequent initiation of dental caries.
27275603	5	33	theme	microscopic	788:798	arg1	methods					835:841	microbiological, biochemical, laser scanning confocal fluorescence microscopic, and scanning electron microscopic methods	721:841	microbiological, biochemical, laser scanning confocal fluorescence microscopic, and scanning electron microscopic methods	721:841	The adherence (in 4-hour biofilms) and biofilm composition (in 46-hour biofilms) of the biofilms were analyzed using microbiological, biochemical, laser scanning confocal fluorescence microscopic, and scanning electron microscopic methods.
27275603	8	34	theme	EPS	1295:1297	arg1	bio-volume					1299:1308	EPS bio-volume	1295:1308	EPS bio-volume	1295:1308	The bacterial and EPS bio-volume and thickness in the biofilms also gradually increased and then decreased as sucrose concentration increased.
27275603	4	35	theme	culture	520:526	arg1	medium					528:533	culture medium	520:533	culture medium containing 0, 0.05, 0.1, 0.5, 1, 2, 5, 10, 20, or 40% (w/v) sucrose	520:601	S. mutans biofilms were formed on saliva-coated hydroxyapatite discs in culture medium containing 0, 0.05, 0.1, 0.5, 1, 2, 5, 10, 20, or 40% (w/v) sucrose.
27275603	4	36	contain	containing	535:544	arg2	sucrose					595:601	0, 0.05, 0.1, 0.5, 1, 2, 5, 10, 20, or 40% (w/v) sucrose	546:601	0, 0.05, 0.1, 0.5, 1, 2, 5, 10, 20, or 40% (w/v) sucrose	546:601	S. mutans biofilms were formed on saliva-coated hydroxyapatite discs in culture medium containing 0, 0.05, 0.1, 0.5, 1, 2, 5, 10, 20, or 40% (w/v) sucrose.
27275603	4	36	contain	containing	535:544	arg1	medium					528:533	culture medium	520:533	culture medium containing 0, 0.05, 0.1, 0.5, 1, 2, 5, 10, 20, or 40% (w/v) sucrose	520:601	S. mutans biofilms were formed on saliva-coated hydroxyapatite discs in culture medium containing 0, 0.05, 0.1, 0.5, 1, 2, 5, 10, 20, or 40% (w/v) sucrose.
27275603	5	37	theme	46-hour	667:673	arg1	biofilms					675:682	46-hour biofilms	667:682	46-hour biofilms	667:682	The adherence (in 4-hour biofilms) and biofilm composition (in 46-hour biofilms) of the biofilms were analyzed using microbiological, biochemical, laser scanning confocal fluorescence microscopic, and scanning electron microscopic methods.
27275603	7	38	theme	2nd	1134:1136	arg1	curve					1155:1159	a 2nd order polynomial curve	1132:1159	a 2nd order polynomial curve with concentration dependence	1132:1189	In this study, the influence of sucrose on bacterial adhesion, biofilm composition (dry weight, bacterial counts, and water-insoluble extracellular polysaccharide (EPS) content), and acidogenicity followed a 2nd order polynomial curve with concentration dependence, and the maximum effective concentrations (MECs) of sucrose ranged from 0.45 to 2.4%.
27275603	7	39	theme	sucrose	1243:1249	arg1	MECs					1234:1237	MECs	1234:1237	MECs	1234:1237	In this study, the influence of sucrose on bacterial adhesion, biofilm composition (dry weight, bacterial counts, and water-insoluble extracellular polysaccharide (EPS) content), and acidogenicity followed a 2nd order polynomial curve with concentration dependence, and the maximum effective concentrations (MECs) of sucrose ranged from 0.45 to 2.4%.
27275603	7	39	theme	sucrose	1243:1249	arg1	concentrations					1218:1231	the maximum effective concentrations	1196:1231	the maximum effective concentrations (MECs) of sucrose	1196:1249	In this study, the influence of sucrose on bacterial adhesion, biofilm composition (dry weight, bacterial counts, and water-insoluble extracellular polysaccharide (EPS) content), and acidogenicity followed a 2nd order polynomial curve with concentration dependence, and the maximum effective concentrations (MECs) of sucrose ranged from 0.45 to 2.4%.
27275603	5	40	theme	microscopic	823:833	arg1	methods					835:841	microbiological, biochemical, laser scanning confocal fluorescence microscopic, and scanning electron microscopic methods	721:841	microbiological, biochemical, laser scanning confocal fluorescence microscopic, and scanning electron microscopic methods	721:841	The adherence (in 4-hour biofilms) and biofilm composition (in 46-hour biofilms) of the biofilms were analyzed using microbiological, biochemical, laser scanning confocal fluorescence microscopic, and scanning electron microscopic methods.
27275603	5	41	theme	biochemical	738:748	arg1	methods					835:841	microbiological, biochemical, laser scanning confocal fluorescence microscopic, and scanning electron microscopic methods	721:841	microbiological, biochemical, laser scanning confocal fluorescence microscopic, and scanning electron microscopic methods	721:841	The adherence (in 4-hour biofilms) and biofilm composition (in 46-hour biofilms) of the biofilms were analyzed using microbiological, biochemical, laser scanning confocal fluorescence microscopic, and scanning electron microscopic methods.
27275603	2	42	theme	sucrose	252:258	arg1	concentration					260:272	sucrose concentration	252:272	sucrose concentration	252:272	This study investigated the functional relationships between sucrose concentration and Streptococcus mutans adherence and biofilm formation.
27275603	5	43	theme	biofilms	692:699	arg1	adherence					608:616	The adherence	604:616	The adherence (in 4-hour biofilms)	604:637	The adherence (in 4-hour biofilms) and biofilm composition (in 46-hour biofilms) of the biofilms were analyzed using microbiological, biochemical, laser scanning confocal fluorescence microscopic, and scanning electron microscopic methods.
27275603	5	43	theme	biofilms	692:699	arg1	composition					651:661	biofilm composition	643:661	biofilm composition (in 46-hour biofilms)	643:683	The adherence (in 4-hour biofilms) and biofilm composition (in 46-hour biofilms) of the biofilms were analyzed using microbiological, biochemical, laser scanning confocal fluorescence microscopic, and scanning electron microscopic methods.
27275603	0	44	theme	Biofilm	57:63	arg1	Formation					65:73	a Cariogenic Biofilm Formation	44:73	a Cariogenic Biofilm Formation	44:73	Functional Relationship between Sucrose and a Cariogenic Biofilm Formation.
27275603	11	45	theme	cariogenic	1764:1773	arg1	formation					1783:1791	cariogenic biofilm formation	1764:1791	cariogenic biofilm formation	1764:1791	These results suggest that the relationship between sucrose concentration and cariogenic biofilm formation in the oral cavity could be described by a functional relationship.
27275603	3	46	theme	morphological	343:355	arg1	characteristics					357:371	morphological characteristics	343:371	morphological characteristics of the biofilms with increasing sucrose concentration	343:425	Changes in morphological characteristics of the biofilms with increasing sucrose concentration were also evaluated.
27275603	7	47	with	curve	1155:1159	arg1	dependence					1180:1189	concentration dependence	1166:1189	concentration dependence	1166:1189	In this study, the influence of sucrose on bacterial adhesion, biofilm composition (dry weight, bacterial counts, and water-insoluble extracellular polysaccharide (EPS) content), and acidogenicity followed a 2nd order polynomial curve with concentration dependence, and the maximum effective concentrations (MECs) of sucrose ranged from 0.45 to 2.4%.
27275603	7	48	theme	biofilm	989:995	arg1	composition					997:1007	biofilm composition	989:1007	biofilm composition (dry weight, bacterial counts, and water-insoluble extracellular polysaccharide (EPS) content)	989:1102	In this study, the influence of sucrose on bacterial adhesion, biofilm composition (dry weight, bacterial counts, and water-insoluble extracellular polysaccharide (EPS) content), and acidogenicity followed a 2nd order polynomial curve with concentration dependence, and the maximum effective concentrations (MECs) of sucrose ranged from 0.45 to 2.4%.
27275603	0	49	theme	Cariogenic	46:55	arg1	Formation					65:73	a Cariogenic Biofilm Formation	44:73	a Cariogenic Biofilm Formation	44:73	Functional Relationship between Sucrose and a Cariogenic Biofilm Formation.
27275603	11	50	theme	sucrose	1738:1744	arg1	concentration					1746:1758	sucrose concentration	1738:1758	sucrose concentration	1738:1758	These results suggest that the relationship between sucrose concentration and cariogenic biofilm formation in the oral cavity could be described by a functional relationship.
27275603	7	51	theme	dry	1010:1012	arg1	weight					1014:1019	dry weight	1010:1019	dry weight	1010:1019	In this study, the influence of sucrose on bacterial adhesion, biofilm composition (dry weight, bacterial counts, and water-insoluble extracellular polysaccharide (EPS) content), and acidogenicity followed a 2nd order polynomial curve with concentration dependence, and the maximum effective concentrations (MECs) of sucrose ranged from 0.45 to 2.4%.
27275603	2	52	theme	functional	219:228	arg1	relationships					230:242	the functional relationships	215:242	the functional relationships between sucrose concentration and Streptococcus mutans adherence and biofilm formation	215:329	This study investigated the functional relationships between sucrose concentration and Streptococcus mutans adherence and biofilm formation.
27275603	1	53	theme	cariogenic	118:127	arg1	formation					137:145	cariogenic biofilm formation	118:145	cariogenic biofilm formation	118:145	Sucrose is an important dietary factor in cariogenic biofilm formation and subsequent initiation of dental caries.
27275603	5	54	theme	biofilm	643:649	arg1	composition					651:661	biofilm composition	643:661	biofilm composition (in 46-hour biofilms)	643:683	The adherence (in 4-hour biofilms) and biofilm composition (in 46-hour biofilms) of the biofilms were analyzed using microbiological, biochemical, laser scanning confocal fluorescence microscopic, and scanning electron microscopic methods.
27275603	11	55	from	relationship	1717:1728	arg1	cavity					1805:1810	the oral cavity	1796:1810	the oral cavity	1796:1810	These results suggest that the relationship between sucrose concentration and cariogenic biofilm formation in the oral cavity could be described by a functional relationship.
27275603	7	56	theme	bacterial	969:977	arg1	adhesion					979:986	bacterial adhesion	969:986	bacterial adhesion	969:986	In this study, the influence of sucrose on bacterial adhesion, biofilm composition (dry weight, bacterial counts, and water-insoluble extracellular polysaccharide (EPS) content), and acidogenicity followed a 2nd order polynomial curve with concentration dependence, and the maximum effective concentrations (MECs) of sucrose ranged from 0.45 to 2.4%.
27275603	1	57	theme	biofilm	129:135	arg1	formation					137:145	cariogenic biofilm formation	118:145	cariogenic biofilm formation	118:145	Sucrose is an important dietary factor in cariogenic biofilm formation and subsequent initiation of dental caries.
27275603	1	58	from	factor	108:113	arg1	formation					137:145	cariogenic biofilm formation	118:145	cariogenic biofilm formation	118:145	Sucrose is an important dietary factor in cariogenic biofilm formation and subsequent initiation of dental caries.
27275603	1	58	from	factor	108:113	arg1	initiation					162:171	subsequent initiation	151:171	subsequent initiation	151:171	Sucrose is an important dietary factor in cariogenic biofilm formation and subsequent initiation of dental caries.
27275603	3	59	from	Changes	332:338	arg1	characteristics					357:371	morphological characteristics	343:371	morphological characteristics of the biofilms with increasing sucrose concentration	343:425	Changes in morphological characteristics of the biofilms with increasing sucrose concentration were also evaluated.
27275603	9	60	theme	biofilms	1481:1488	arg1	micro-colonies					1459:1472	the micro-colonies	1455:1472	the micro-colonies of the biofilms	1455:1488	Furthermore, the size and shape of the micro-colonies of the biofilms depended on the sucrose concentration.
27275603	2	61	theme	mutans	292:297	arg1	adherence					299:307	Streptococcus mutans adherence	278:307	Streptococcus mutans adherence	278:307	This study investigated the functional relationships between sucrose concentration and Streptococcus mutans adherence and biofilm formation.
27275603	7	62	theme	polysaccharide	1074:1087	arg1	content					1095:1101	water-insoluble extracellular polysaccharide (EPS) content	1044:1101	water-insoluble extracellular polysaccharide (EPS) content	1044:1101	In this study, the influence of sucrose on bacterial adhesion, biofilm composition (dry weight, bacterial counts, and water-insoluble extracellular polysaccharide (EPS) content), and acidogenicity followed a 2nd order polynomial curve with concentration dependence, and the maximum effective concentrations (MECs) of sucrose ranged from 0.45 to 2.4%.
27275603	9	63	theme	micro-colonies	1459:1472	arg1	shape					1446:1450	shape	1446:1450	shape	1446:1450	Furthermore, the size and shape of the micro-colonies of the biofilms depended on the sucrose concentration.
27275603	9	63	theme	micro-colonies	1459:1472	arg1	size					1437:1440	size	1437:1440	size	1437:1440	Furthermore, the size and shape of the micro-colonies of the biofilms depended on the sucrose concentration.
27275603	4	64	theme	%	587:587	arg1	sucrose					595:601	0, 0.05, 0.1, 0.5, 1, 2, 5, 10, 20, or 40% (w/v) sucrose	546:601	0, 0.05, 0.1, 0.5, 1, 2, 5, 10, 20, or 40% (w/v) sucrose	546:601	S. mutans biofilms were formed on saliva-coated hydroxyapatite discs in culture medium containing 0, 0.05, 0.1, 0.5, 1, 2, 5, 10, 20, or 40% (w/v) sucrose.
27275603	3	65	theme	biofilms	380:387	arg1	characteristics					357:371	morphological characteristics	343:371	morphological characteristics of the biofilms with increasing sucrose concentration	343:425	Changes in morphological characteristics of the biofilms with increasing sucrose concentration were also evaluated.
27275603	4	66	theme	hydroxyapatite	496:509	arg1	discs					511:515	saliva-coated hydroxyapatite discs	482:515	saliva-coated hydroxyapatite discs in culture medium containing 0, 0.05, 0.1, 0.5, 1, 2, 5, 10, 20, or 40% (w/v) sucrose	482:601	S. mutans biofilms were formed on saliva-coated hydroxyapatite discs in culture medium containing 0, 0.05, 0.1, 0.5, 1, 2, 5, 10, 20, or 40% (w/v) sucrose.
27275603	8	67	theme	bacterial	1281:1289	arg1	bio-volume					1299:1308	EPS bio-volume	1295:1308	EPS bio-volume	1295:1308	The bacterial and EPS bio-volume and thickness in the biofilms also gradually increased and then decreased as sucrose concentration increased.
27275603	7	68	theme	sucrose	958:964	arg1	influence					945:953	the influence	941:953	the influence of sucrose on bacterial adhesion, biofilm composition (dry weight, bacterial counts, and water-insoluble extracellular polysaccharide (EPS) content), and acidogenicity	941:1121	In this study, the influence of sucrose on bacterial adhesion, biofilm composition (dry weight, bacterial counts, and water-insoluble extracellular polysaccharide (EPS) content), and acidogenicity followed a 2nd order polynomial curve with concentration dependence, and the maximum effective concentrations (MECs) of sucrose ranged from 0.45 to 2.4%.
27275603	7	69	theme	effective	1208:1216	arg1	MECs					1234:1237	MECs	1234:1237	MECs	1234:1237	In this study, the influence of sucrose on bacterial adhesion, biofilm composition (dry weight, bacterial counts, and water-insoluble extracellular polysaccharide (EPS) content), and acidogenicity followed a 2nd order polynomial curve with concentration dependence, and the maximum effective concentrations (MECs) of sucrose ranged from 0.45 to 2.4%.
27275603	7	69	theme	effective	1208:1216	arg1	concentrations					1218:1231	the maximum effective concentrations	1196:1231	the maximum effective concentrations (MECs) of sucrose	1196:1249	In this study, the influence of sucrose on bacterial adhesion, biofilm composition (dry weight, bacterial counts, and water-insoluble extracellular polysaccharide (EPS) content), and acidogenicity followed a 2nd order polynomial curve with concentration dependence, and the maximum effective concentrations (MECs) of sucrose ranged from 0.45 to 2.4%.
27275603	7	70	from	influence	945:953	arg1	adhesion					979:986	bacterial adhesion	969:986	bacterial adhesion	969:986	In this study, the influence of sucrose on bacterial adhesion, biofilm composition (dry weight, bacterial counts, and water-insoluble extracellular polysaccharide (EPS) content), and acidogenicity followed a 2nd order polynomial curve with concentration dependence, and the maximum effective concentrations (MECs) of sucrose ranged from 0.45 to 2.4%.
27275603	7	70	from	influence	945:953	arg1	composition					997:1007	biofilm composition	989:1007	biofilm composition (dry weight, bacterial counts, and water-insoluble extracellular polysaccharide (EPS) content)	989:1102	In this study, the influence of sucrose on bacterial adhesion, biofilm composition (dry weight, bacterial counts, and water-insoluble extracellular polysaccharide (EPS) content), and acidogenicity followed a 2nd order polynomial curve with concentration dependence, and the maximum effective concentrations (MECs) of sucrose ranged from 0.45 to 2.4%.
27275603	7	70	from	influence	945:953	arg1	acidogenicity					1109:1121	acidogenicity	1109:1121	acidogenicity	1109:1121	In this study, the influence of sucrose on bacterial adhesion, biofilm composition (dry weight, bacterial counts, and water-insoluble extracellular polysaccharide (EPS) content), and acidogenicity followed a 2nd order polynomial curve with concentration dependence, and the maximum effective concentrations (MECs) of sucrose ranged from 0.45 to 2.4%.
27275603	6	71	theme	curve	897:901	arg1	fitting					903:909	2nd order polynomial curve fitting	876:909	2nd order polynomial curve fitting	876:909	To determine the relationships, 2nd order polynomial curve fitting was performed.
27275603	1	72	theme	subsequent	151:160	arg1	initiation					162:171	subsequent initiation	151:171	subsequent initiation	151:171	Sucrose is an important dietary factor in cariogenic biofilm formation and subsequent initiation of dental caries.
27275603	7	73	dep	composition	997:1007	arg1	content					1095:1101	water-insoluble extracellular polysaccharide (EPS) content	1044:1101	water-insoluble extracellular polysaccharide (EPS) content	1044:1101	In this study, the influence of sucrose on bacterial adhesion, biofilm composition (dry weight, bacterial counts, and water-insoluble extracellular polysaccharide (EPS) content), and acidogenicity followed a 2nd order polynomial curve with concentration dependence, and the maximum effective concentrations (MECs) of sucrose ranged from 0.45 to 2.4%.
27275603	7	73	dep	composition	997:1007	arg1	weight					1014:1019	dry weight	1010:1019	dry weight	1010:1019	In this study, the influence of sucrose on bacterial adhesion, biofilm composition (dry weight, bacterial counts, and water-insoluble extracellular polysaccharide (EPS) content), and acidogenicity followed a 2nd order polynomial curve with concentration dependence, and the maximum effective concentrations (MECs) of sucrose ranged from 0.45 to 2.4%.
27275603	7	73	dep	composition	997:1007	arg1	counts					1032:1037	bacterial counts	1022:1037	bacterial counts	1022:1037	In this study, the influence of sucrose on bacterial adhesion, biofilm composition (dry weight, bacterial counts, and water-insoluble extracellular polysaccharide (EPS) content), and acidogenicity followed a 2nd order polynomial curve with concentration dependence, and the maximum effective concentrations (MECs) of sucrose ranged from 0.45 to 2.4%.
24965000	5	0	theme	simulated	585:593	arg1	urine					595:599	simulated urine	585:599	simulated urine	585:599	In vitro studies carried out with simulated urine demonstrated that the tubes present a high fluid uptake ability, about 1000%.
24965000	11	1	theme	higher	1384:1389	arg1	inhibition					1391:1400	a higher inhibition	1382:1400	a higher inhibition of bacterial adhesion	1382:1422	For K. oxytoca, no differences were observed in comparison with a commercial stent (Biosoft(®) duo, Porges), although, for S. aureus all tested compositions had a higher inhibition of bacterial adhesion compared to the commercial stents.
24965000	8	2	theme	60	969:970	arg1	days					972:975	14 and 60 days	962:975	days	972:975	Complete dissolution of the materials may occur between 14 and 60 days.
24965000	4	3	theme	stents	471:476	arg1	encrustation					436:447	encrustation	436:447	encrustation	436:447	Indwelling time, encrustation, and stability of the stents in artificial urine solution was carried out up to 60 days of immersion.
24965000	4	3	theme	stents	471:476	arg1	stability					454:462	stability	454:462	stability	454:462	Indwelling time, encrustation, and stability of the stents in artificial urine solution was carried out up to 60 days of immersion.
24965000	4	3	theme	stents	471:476	arg1	time					430:433	Indwelling time	419:433	Indwelling time	419:433	Indwelling time, encrustation, and stability of the stents in artificial urine solution was carried out up to 60 days of immersion.
24965000	11	4	theme	bacterial	1405:1413	arg1	adhesion					1415:1422	bacterial adhesion	1405:1422	bacterial adhesion	1405:1422	For K. oxytoca, no differences were observed in comparison with a commercial stent (Biosoft(®) duo, Porges), although, for S. aureus all tested compositions had a higher inhibition of bacterial adhesion compared to the commercial stents.
24965000	8	5	theme	materials	934:942	arg1	dissolution					915:925	Complete dissolution	906:925	Complete dissolution of the materials	906:942	Complete dissolution of the materials may occur between 14 and 60 days.
24965000	6	6	theme	extensive	762:770	arg1	behavior					781:788	an extensive swelling behavior	759:788	an extensive swelling behavior	759:788	Despite this, the materials are able to maintain their shape and do not present an extensive swelling behavior.
24965000	3	7	theme	Morphological	318:330	arg1	analysis					332:339	Morphological analysis	318:339	Morphological analysis of the surface of the stents	318:368	Morphological analysis of the surface of the stents was carried out by scanning electron microscopy.
24965000	7	8	theme	stent	879:883	arg1	composition					860:870	the composition	856:870	the composition of the stent	856:883	The bioresorption profile was observed to be highly dependent on the composition of the stent and it can be tuned.
24965000	4	9	from	stability	454:462	arg1	solution					498:505	artificial urine solution	481:505	artificial urine solution	481:505	Indwelling time, encrustation, and stability of the stents in artificial urine solution was carried out up to 60 days of immersion.
24965000	5	10	theme	In	551:552	arg1	studies					560:566	In vitro studies	551:566	In vitro studies carried out with simulated urine	551:599	In vitro studies carried out with simulated urine demonstrated that the tubes present a high fluid uptake ability, about 1000%.
24965000	2	11	theme	critical	280:287	arg1	dioxide					302:308	critical point carbon dioxide	280:308	critical point carbon dioxide	280:308	Alginate, gellan gum, and a blend of these with gelatin were used to produce hollow tube (stents) following a combination of templated gelation and critical point carbon dioxide drying.
24965000	2	12	theme	gellan	142:147	arg1	gum					149:151	gellan gum	142:151	gellan gum	142:151	Alginate, gellan gum, and a blend of these with gelatin were used to produce hollow tube (stents) following a combination of templated gelation and critical point carbon dioxide drying.
24965000	11	13	with	comparison	1269:1278	arg1	stent					1298:1302	a commercial stent	1285:1302	a commercial stent (Biosoft(®) duo, Porges)	1285:1327	For K. oxytoca, no differences were observed in comparison with a commercial stent (Biosoft(®) duo, Porges), although, for S. aureus all tested compositions had a higher inhibition of bacterial adhesion compared to the commercial stents.
24965000	3	14	theme	scanning	389:396	arg1	microscopy					407:416	scanning electron microscopy	389:416	scanning electron microscopy	389:416	Morphological analysis of the surface of the stents was carried out by scanning electron microscopy.
24965000	13	15	theme	developed	1647:1655	arg1	technology					1657:1666	the developed technology	1643:1666	the developed technology	1643:1666	The stents produced by the developed technology fulfill the requirements for ureteral stents and will contribute in the development of biocompatible and bioresorbable urinary stents.
24965000	13	16	theme	bioresorbable	1773:1785	arg1	stents					1795:1800	biocompatible and bioresorbable urinary stents	1755:1800	biocompatible and bioresorbable urinary stents	1755:1800	The stents produced by the developed technology fulfill the requirements for ureteral stents and will contribute in the development of biocompatible and bioresorbable urinary stents.
24965000	10	17	dep	aureus	1140:1145	arg1	alpha					1191:1195	DH5 alpha	1187:1195	DH5 alpha	1187:1195	The ability to resist bacterial adherence was evaluated with Gram-positive Staphylococcus aureus and two Gram-negatives Escherichia coli DH5 alpha and Klebsiella oxytoca.
24965000	10	18	theme	bacterial	1072:1080	arg1	adherence					1082:1090	bacterial adherence	1072:1090	bacterial adherence	1072:1090	The ability to resist bacterial adherence was evaluated with Gram-positive Staphylococcus aureus and two Gram-negatives Escherichia coli DH5 alpha and Klebsiella oxytoca.
24965000	13	19	theme	ureteral	1697:1704	arg1	stents					1706:1711	ureteral stents	1697:1711	ureteral stents	1697:1711	The stents produced by the developed technology fulfill the requirements for ureteral stents and will contribute in the development of biocompatible and bioresorbable urinary stents.
24965000	3	20	theme	electron	398:405	arg1	microscopy					407:416	scanning electron microscopy	389:416	scanning electron microscopy	389:416	Morphological analysis of the surface of the stents was carried out by scanning electron microscopy.
24965000	3	21	theme	surface	348:354	arg1	analysis					332:339	Morphological analysis	318:339	Morphological analysis of the surface of the stents	318:368	Morphological analysis of the surface of the stents was carried out by scanning electron microscopy.
24965000	11	22	theme	commercial	1287:1296	arg1	stent					1298:1302	a commercial stent	1285:1302	a commercial stent (Biosoft(®) duo, Porges)	1285:1327	For K. oxytoca, no differences were observed in comparison with a commercial stent (Biosoft(®) duo, Porges), although, for S. aureus all tested compositions had a higher inhibition of bacterial adhesion compared to the commercial stents.
24965000	4	23	from	encrustation	436:447	arg1	solution					498:505	artificial urine solution	481:505	artificial urine solution	481:505	Indwelling time, encrustation, and stability of the stents in artificial urine solution was carried out up to 60 days of immersion.
24965000	11	24	theme	commercial	1440:1449	arg1	stents					1451:1456	the commercial stents	1436:1456	the commercial stents	1436:1456	For K. oxytoca, no differences were observed in comparison with a commercial stent (Biosoft(®) duo, Porges), although, for S. aureus all tested compositions had a higher inhibition of bacterial adhesion compared to the commercial stents.
24965000	0	25	from	polymers	50:57	arg1	stents					23:28	ureteral stents	14:28	ureteral stents from natural origin polymers	14:57	Bioresorbable ureteral stents from natural origin polymers.
24965000	11	26	dep	stent	1298:1302	arg1	®					1313:1313	®	1313:1313	®	1313:1313	For K. oxytoca, no differences were observed in comparison with a commercial stent (Biosoft(®) duo, Porges), although, for S. aureus all tested compositions had a higher inhibition of bacterial adhesion compared to the commercial stents.
24965000	11	26	dep	stent	1298:1302	arg1	Biosoft					1305:1311	Biosoft	1305:1311	Biosoft	1305:1311	For K. oxytoca, no differences were observed in comparison with a commercial stent (Biosoft(®) duo, Porges), although, for S. aureus all tested compositions had a higher inhibition of bacterial adhesion compared to the commercial stents.
24965000	8	27	theme	14	962:963	arg1	days					972:975	14 and 60 days	962:975	days	972:975	Complete dissolution of the materials may occur between 14 and 60 days.
24965000	12	28	theme	commercial	1601:1610	arg1	stents					1612:1617	the commercial stents	1597:1617	the commercial stents	1597:1617	In case of E. coli, the addition of gelatin to the formulations reduced the bacterial adhesion in a highly significant manner compared to the commercial stents.
24965000	2	29	theme	gelation	267:274	arg1	combination					242:252	a combination	240:252	a combination of templated gelation and critical point carbon dioxide drying	240:315	Alginate, gellan gum, and a blend of these with gelatin were used to produce hollow tube (stents) following a combination of templated gelation and critical point carbon dioxide drying.
24965000	13	30	theme	urinary	1787:1793	arg1	stents					1795:1800	biocompatible and bioresorbable urinary stents	1755:1800	biocompatible and bioresorbable urinary stents	1755:1800	The stents produced by the developed technology fulfill the requirements for ureteral stents and will contribute in the development of biocompatible and bioresorbable urinary stents.
24965000	9	31	located	observed	1012:1019	arg2	encrustation					995:1006	no encrustation	992:1006	no encrustation	992:1006	Additionally, no encrustation was observed within the tested timeframe.
24965000	9	31	located	observed	1012:1019	arg1	timeframe					1039:1047	the tested timeframe	1028:1047	the tested timeframe	1028:1047	Additionally, no encrustation was observed within the tested timeframe.
24965000	4	32	from	time	430:433	arg1	solution					498:505	artificial urine solution	481:505	artificial urine solution	481:505	Indwelling time, encrustation, and stability of the stents in artificial urine solution was carried out up to 60 days of immersion.
24965000	8	33	theme	Complete	906:913	arg1	dissolution					915:925	Complete dissolution	906:925	Complete dissolution of the materials	906:942	Complete dissolution of the materials may occur between 14 and 60 days.
24965000	2	34	theme	templated	257:265	arg1	gelation					267:274	templated gelation	257:274	templated gelation	257:274	Alginate, gellan gum, and a blend of these with gelatin were used to produce hollow tube (stents) following a combination of templated gelation and critical point carbon dioxide drying.
24965000	2	35	used	used	193:196	arg2	gum					149:151	gellan gum	142:151	gellan gum	142:151	Alginate, gellan gum, and a blend of these with gelatin were used to produce hollow tube (stents) following a combination of templated gelation and critical point carbon dioxide drying.
24965000	2	35	used	used	193:196	arg2	blend					160:164	a blend	158:164	a blend of these with gelatin	158:186	Alginate, gellan gum, and a blend of these with gelatin were used to produce hollow tube (stents) following a combination of templated gelation and critical point carbon dioxide drying.
24965000	2	35	used	used	193:196	arg2	Alginate					132:139	Alginate	132:139	Alginate	132:139	Alginate, gellan gum, and a blend of these with gelatin were used to produce hollow tube (stents) following a combination of templated gelation and critical point carbon dioxide drying.
24965000	5	36	theme	fluid	644:648	arg1	%					676:676	about 1000%	666:676	about 1000%	666:676	In vitro studies carried out with simulated urine demonstrated that the tubes present a high fluid uptake ability, about 1000%.
24965000	5	36	theme	fluid	644:648	arg1	ability					657:663	a high fluid uptake ability	637:663	a high fluid uptake ability	637:663	In vitro studies carried out with simulated urine demonstrated that the tubes present a high fluid uptake ability, about 1000%.
24965000	11	37	contain	had	1378:1380	arg2	inhibition					1391:1400	a higher inhibition	1382:1400	a higher inhibition of bacterial adhesion	1382:1422	For K. oxytoca, no differences were observed in comparison with a commercial stent (Biosoft(®) duo, Porges), although, for S. aureus all tested compositions had a higher inhibition of bacterial adhesion compared to the commercial stents.
24965000	11	37	contain	had	1378:1380	arg1	compositions					1365:1376	all tested compositions	1354:1376	all tested compositions	1354:1376	For K. oxytoca, no differences were observed in comparison with a commercial stent (Biosoft(®) duo, Porges), although, for S. aureus all tested compositions had a higher inhibition of bacterial adhesion compared to the commercial stents.
24965000	12	38	theme	gelatin	1495:1501	arg1	addition					1483:1490	the addition	1479:1490	the addition of gelatin to the formulations	1479:1521	In case of E. coli, the addition of gelatin to the formulations reduced the bacterial adhesion in a highly significant manner compared to the commercial stents.
24965000	0	39	theme	origin	43:48	arg1	polymers					50:57	natural origin polymers	35:57	natural origin polymers	35:57	Bioresorbable ureteral stents from natural origin polymers.
24965000	2	40	theme	carbon	295:300	arg1	dioxide					302:308	critical point carbon dioxide	280:308	critical point carbon dioxide	280:308	Alginate, gellan gum, and a blend of these with gelatin were used to produce hollow tube (stents) following a combination of templated gelation and critical point carbon dioxide drying.
24965000	5	41	theme	uptake	650:655	arg1	%					676:676	about 1000%	666:676	about 1000%	666:676	In vitro studies carried out with simulated urine demonstrated that the tubes present a high fluid uptake ability, about 1000%.
24965000	5	41	theme	uptake	650:655	arg1	ability					657:663	a high fluid uptake ability	637:663	a high fluid uptake ability	637:663	In vitro studies carried out with simulated urine demonstrated that the tubes present a high fluid uptake ability, about 1000%.
24965000	6	42	theme	swelling	772:779	arg1	behavior					781:788	an extensive swelling behavior	759:788	an extensive swelling behavior	759:788	Despite this, the materials are able to maintain their shape and do not present an extensive swelling behavior.
24965000	12	43	theme	bacterial	1535:1543	arg1	adhesion					1545:1552	the bacterial adhesion	1531:1552	the bacterial adhesion	1531:1552	In case of E. coli, the addition of gelatin to the formulations reduced the bacterial adhesion in a highly significant manner compared to the commercial stents.
24965000	11	44	theme	adhesion	1415:1422	arg1	inhibition					1391:1400	a higher inhibition	1382:1400	a higher inhibition of bacterial adhesion	1382:1422	For K. oxytoca, no differences were observed in comparison with a commercial stent (Biosoft(®) duo, Porges), although, for S. aureus all tested compositions had a higher inhibition of bacterial adhesion compared to the commercial stents.
24965000	0	45	theme	natural	35:41	arg1	polymers					50:57	natural origin polymers	35:57	natural origin polymers	35:57	Bioresorbable ureteral stents from natural origin polymers.
24965000	4	46	theme	artificial	481:490	arg1	solution					498:505	artificial urine solution	481:505	artificial urine solution	481:505	Indwelling time, encrustation, and stability of the stents in artificial urine solution was carried out up to 60 days of immersion.
24965000	4	47	dep	60	529:530	arg1	to					526:527	to	526:527	to	526:527	Indwelling time, encrustation, and stability of the stents in artificial urine solution was carried out up to 60 days of immersion.
24965000	13	48	theme	biocompatible	1755:1767	arg1	stents					1795:1800	biocompatible and bioresorbable urinary stents	1755:1800	biocompatible and bioresorbable urinary stents	1755:1800	The stents produced by the developed technology fulfill the requirements for ureteral stents and will contribute in the development of biocompatible and bioresorbable urinary stents.
24965000	11	49	dep	Biosoft	1305:1311	arg1	Porges					1321:1326	Porges	1321:1326	Porges	1321:1326	For K. oxytoca, no differences were observed in comparison with a commercial stent (Biosoft(®) duo, Porges), although, for S. aureus all tested compositions had a higher inhibition of bacterial adhesion compared to the commercial stents.
24965000	4	50	theme	Indwelling	419:428	arg1	time					430:433	Indwelling time	419:433	Indwelling time	419:433	Indwelling time, encrustation, and stability of the stents in artificial urine solution was carried out up to 60 days of immersion.
24965000	2	51	theme	dioxide	302:308	arg1	combination					242:252	a combination	240:252	a combination of templated gelation and critical point carbon dioxide drying	240:315	Alginate, gellan gum, and a blend of these with gelatin were used to produce hollow tube (stents) following a combination of templated gelation and critical point carbon dioxide drying.
24965000	3	52	theme	stents	363:368	arg1	surface					348:354	the surface	344:354	the surface of the stents	344:368	Morphological analysis of the surface of the stents was carried out by scanning electron microscopy.
24965000	1	53	theme	natural	100:106	arg1	polysaccharides					115:129	natural origin polysaccharides	100:129	natural origin polysaccharides	100:129	In this work, stents were produced from natural origin polysaccharides.
24965000	5	54	theme	high	639:642	arg1	%					676:676	about 1000%	666:676	about 1000%	666:676	In vitro studies carried out with simulated urine demonstrated that the tubes present a high fluid uptake ability, about 1000%.
24965000	5	54	theme	high	639:642	arg1	ability					657:663	a high fluid uptake ability	637:663	a high fluid uptake ability	637:663	In vitro studies carried out with simulated urine demonstrated that the tubes present a high fluid uptake ability, about 1000%.
24965000	1	55	theme	origin	108:113	arg1	polysaccharides					115:129	natural origin polysaccharides	100:129	natural origin polysaccharides	100:129	In this work, stents were produced from natural origin polysaccharides.
24965000	5	56	dep	In	551:552	arg1	vitro					554:558	vitro	554:558	vitro	554:558	In vitro studies carried out with simulated urine demonstrated that the tubes present a high fluid uptake ability, about 1000%.
24965000	4	57	theme	urine	492:496	arg1	solution					498:505	artificial urine solution	481:505	artificial urine solution	481:505	Indwelling time, encrustation, and stability of the stents in artificial urine solution was carried out up to 60 days of immersion.
24965000	9	58	theme	tested	1032:1037	arg1	timeframe					1039:1047	the tested timeframe	1028:1047	the tested timeframe	1028:1047	Additionally, no encrustation was observed within the tested timeframe.
24965000	2	59	theme	point	289:293	arg1	dioxide					302:308	critical point carbon dioxide	280:308	critical point carbon dioxide	280:308	Alginate, gellan gum, and a blend of these with gelatin were used to produce hollow tube (stents) following a combination of templated gelation and critical point carbon dioxide drying.
24965000	11	60	theme	tested	1358:1363	arg1	compositions					1365:1376	all tested compositions	1354:1376	all tested compositions	1354:1376	For K. oxytoca, no differences were observed in comparison with a commercial stent (Biosoft(®) duo, Porges), although, for S. aureus all tested compositions had a higher inhibition of bacterial adhesion compared to the commercial stents.
24965000	11	61	located	observed	1257:1264	arg1	comparison					1269:1278	comparison	1269:1278	comparison with a commercial stent (Biosoft(®) duo, Porges)	1269:1327	For K. oxytoca, no differences were observed in comparison with a commercial stent (Biosoft(®) duo, Porges), although, for S. aureus all tested compositions had a higher inhibition of bacterial adhesion compared to the commercial stents.
24965000	11	61	located	observed	1257:1264	arg2	differences					1240:1250	no differences	1237:1250	no differences	1237:1250	For K. oxytoca, no differences were observed in comparison with a commercial stent (Biosoft(®) duo, Porges), although, for S. aureus all tested compositions had a higher inhibition of bacterial adhesion compared to the commercial stents.
24965000	2	62	theme	these	169:173	arg1	blend					160:164	a blend	158:164	a blend of these with gelatin	158:186	Alginate, gellan gum, and a blend of these with gelatin were used to produce hollow tube (stents) following a combination of templated gelation and critical point carbon dioxide drying.
24965000	2	62	theme	these	169:173	arg1	Alginate					132:139	Alginate	132:139	Alginate	132:139	Alginate, gellan gum, and a blend of these with gelatin were used to produce hollow tube (stents) following a combination of templated gelation and critical point carbon dioxide drying.
24965000	2	62	theme	these	169:173	arg1	gum					149:151	gellan gum	142:151	gellan gum	142:151	Alginate, gellan gum, and a blend of these with gelatin were used to produce hollow tube (stents) following a combination of templated gelation and critical point carbon dioxide drying.
24965000	2	63	dep	gelation	267:274	arg1	drying					310:315	drying	310:315	drying	310:315	Alginate, gellan gum, and a blend of these with gelatin were used to produce hollow tube (stents) following a combination of templated gelation and critical point carbon dioxide drying.
24965000	13	64	theme	stents	1795:1800	arg1	development					1740:1750	the development	1736:1750	the development of biocompatible and bioresorbable urinary stents	1736:1800	The stents produced by the developed technology fulfill the requirements for ureteral stents and will contribute in the development of biocompatible and bioresorbable urinary stents.
24965000	4	65	theme	immersion	540:548	arg1	days					532:535	up to 60 days	523:535	up to 60 days of immersion	523:548	Indwelling time, encrustation, and stability of the stents in artificial urine solution was carried out up to 60 days of immersion.
24965000	2	66	theme	tube	216:219	arg1	stents					222:227	hollow tube (stents)	209:228	hollow tube (stents)	209:228	Alginate, gellan gum, and a blend of these with gelatin were used to produce hollow tube (stents) following a combination of templated gelation and critical point carbon dioxide drying.
24965000	10	67	theme	DH5	1187:1189	arg1	alpha					1191:1195	DH5 alpha	1187:1195	DH5 alpha	1187:1195	The ability to resist bacterial adherence was evaluated with Gram-positive Staphylococcus aureus and two Gram-negatives Escherichia coli DH5 alpha and Klebsiella oxytoca.
24965000	12	68	theme	significant	1566:1576	arg1	manner					1578:1583	a highly significant manner	1557:1583	a highly significant manner	1557:1583	In case of E. coli, the addition of gelatin to the formulations reduced the bacterial adhesion in a highly significant manner compared to the commercial stents.
24965000	2	69	theme	hollow	209:214	arg1	stents					222:227	hollow tube (stents)	209:228	hollow tube (stents)	209:228	Alginate, gellan gum, and a blend of these with gelatin were used to produce hollow tube (stents) following a combination of templated gelation and critical point carbon dioxide drying.
24965000	0	70	theme	ureteral	14:21	arg1	stents					23:28	ureteral stents	14:28	ureteral stents from natural origin polymers	14:57	Bioresorbable ureteral stents from natural origin polymers.
24965000	7	71	theme	bioresorption	795:807	arg1	profile					809:815	The bioresorption profile	791:815	The bioresorption profile	791:815	The bioresorption profile was observed to be highly dependent on the composition of the stent and it can be tuned.
24965000	7	71	theme	bioresorption	795:807	arg1	dependent					843:851	dependent	843:851	dependent	843:851	The bioresorption profile was observed to be highly dependent on the composition of the stent and it can be tuned.
27783048	3	0	theme	Yuanzhi	474:480	arg1	component					461:469	the index component	451:469	the index component of Yuanzhi (a well-known Traditional Chinese Medicine or TCM)	451:531	In particular the compound 3',6-disinapoylsucrose, the index component of Yuanzhi (a well-known Traditional Chinese Medicine or TCM), presents antidepressant effects at a molecular level, and has become a hotspot of research on new lead drug compounds.
27783048	3	0	theme	Yuanzhi	474:480	arg1	compound					418:425	the compound 3',6-disinapoylsucrose, the index component of Yuanzhi (a well-known Traditional Chinese Medicine or TCM),	414:532	compound	418:425	In particular the compound 3',6-disinapoylsucrose, the index component of Yuanzhi (a well-known Traditional Chinese Medicine or TCM), presents antidepressant effects at a molecular level, and has become a hotspot of research on new lead drug compounds.
27783048	1	1	theme	skeleton	230:237	arg1	part					239:242	a glycosyl skeleton part	219:242	a glycosyl skeleton part through ester bonds	219:262	Cinnamic acid sugar ester derivatives (CASEDs) are a class of natural product with one or several phenylacrylic moieties linked with the non-anomeric carbon of a glycosyl skeleton part through ester bonds.
27783048	3	2	theme	lead	632:635	arg1	compounds					642:650	new lead drug compounds	628:650	new lead drug compounds	628:650	In particular the compound 3',6-disinapoylsucrose, the index component of Yuanzhi (a well-known Traditional Chinese Medicine or TCM), presents antidepressant effects at a molecular level, and has become a hotspot of research on new lead drug compounds.
27783048	6	3	theme	distribution	1068:1079	arg1	discussion					1048:1057	a systematic discussion	1035:1057	a systematic discussion of their distribution	1035:1079	This overview discusses the isolation of cinnamic acid sugar ester derivatives from plants, together with a systematic discussion of their distribution, chemical structures and properties and pharmacological activities, with the hope of providing references for natural product researchers and draw attention to these interesting compounds.
27783048	4	4	theme	traditional	729:739	arg1	medicine					741:748	traditional medicine	729:748	traditional medicine	729:748	Several other similar cinnamic acid sugar ester derivatives are reported in traditional medicine as compounds to calm the nerves and display anti-depression and neuroprotective activity.
27783048	1	5	theme	part	239:242	arg1	carbon					209:214	the non-anomeric carbon	192:214	the non-anomeric carbon of a glycosyl skeleton part through ester bonds	192:262	Cinnamic acid sugar ester derivatives (CASEDs) are a class of natural product with one or several phenylacrylic moieties linked with the non-anomeric carbon of a glycosyl skeleton part through ester bonds.
27783048	6	6	from	isolation	957:965	arg1	activities					1137:1146	pharmacological activities	1121:1146	pharmacological activities	1121:1146	This overview discusses the isolation of cinnamic acid sugar ester derivatives from plants, together with a systematic discussion of their distribution, chemical structures and properties and pharmacological activities, with the hope of providing references for natural product researchers and draw attention to these interesting compounds.
27783048	6	6	from	isolation	957:965	arg1	structures					1091:1100	chemical structures	1082:1100	chemical structures	1082:1100	This overview discusses the isolation of cinnamic acid sugar ester derivatives from plants, together with a systematic discussion of their distribution, chemical structures and properties and pharmacological activities, with the hope of providing references for natural product researchers and draw attention to these interesting compounds.
27783048	6	6	from	isolation	957:965	arg1	plants					1013:1018	plants	1013:1018	plants	1013:1018	This overview discusses the isolation of cinnamic acid sugar ester derivatives from plants, together with a systematic discussion of their distribution, chemical structures and properties and pharmacological activities, with the hope of providing references for natural product researchers and draw attention to these interesting compounds.
27783048	6	6	from	isolation	957:965	arg1	properties					1106:1115	properties	1106:1115	properties	1106:1115	This overview discusses the isolation of cinnamic acid sugar ester derivatives from plants, together with a systematic discussion of their distribution, chemical structures and properties and pharmacological activities, with the hope of providing references for natural product researchers and draw attention to these interesting compounds.
27783048	3	7	theme	drug	637:640	arg1	compounds					642:650	new lead drug compounds	628:650	new lead drug compounds	628:650	In particular the compound 3',6-disinapoylsucrose, the index component of Yuanzhi (a well-known Traditional Chinese Medicine or TCM), presents antidepressant effects at a molecular level, and has become a hotspot of research on new lead drug compounds.
27783048	3	8	dep	compound	418:425	arg1	6-disinapoylsucrose					430:448	6-disinapoylsucrose	430:448	6-disinapoylsucrose	430:448	In particular the compound 3',6-disinapoylsucrose, the index component of Yuanzhi (a well-known Traditional Chinese Medicine or TCM), presents antidepressant effects at a molecular level, and has become a hotspot of research on new lead drug compounds.
27783048	1	9	theme	Cinnamic	59:66	arg1	class					112:116	a class	110:116	a class of natural product	110:135	Cinnamic acid sugar ester derivatives (CASEDs) are a class of natural product with one or several phenylacrylic moieties linked with the non-anomeric carbon of a glycosyl skeleton part through ester bonds.
27783048	1	9	theme	Cinnamic	59:66	arg1	CASEDs					98:103	CASEDs	98:103	CASEDs	98:103	Cinnamic acid sugar ester derivatives (CASEDs) are a class of natural product with one or several phenylacrylic moieties linked with the non-anomeric carbon of a glycosyl skeleton part through ester bonds.
27783048	1	9	theme	Cinnamic	59:66	arg1	derivatives					85:95	Cinnamic acid sugar ester derivatives	59:95	Cinnamic acid sugar ester derivatives (CASEDs)	59:104	Cinnamic acid sugar ester derivatives (CASEDs) are a class of natural product with one or several phenylacrylic moieties linked with the non-anomeric carbon of a glycosyl skeleton part through ester bonds.
27783048	4	10	theme	ester	695:699	arg1	compounds					753:761	compounds	753:761	compounds to calm the nerves and display anti-depression and neuroprotective activity	753:837	Several other similar cinnamic acid sugar ester derivatives are reported in traditional medicine as compounds to calm the nerves and display anti-depression and neuroprotective activity.
27783048	4	10	theme	ester	695:699	arg1	derivatives					701:711	Several other similar cinnamic acid sugar ester derivatives	653:711	Several other similar cinnamic acid sugar ester derivatives	653:711	Several other similar cinnamic acid sugar ester derivatives are reported in traditional medicine as compounds to calm the nerves and display anti-depression and neuroprotective activity.
27783048	3	11	dep	Yuanzhi	474:480	arg1	Medicine					516:523	a well-known Traditional Chinese Medicine	483:523	a well-known Traditional Chinese Medicine	483:523	In particular the compound 3',6-disinapoylsucrose, the index component of Yuanzhi (a well-known Traditional Chinese Medicine or TCM), presents antidepressant effects at a molecular level, and has become a hotspot of research on new lead drug compounds.
27783048	3	11	dep	Yuanzhi	474:480	arg1	TCM					528:530	TCM	528:530	TCM	528:530	In particular the compound 3',6-disinapoylsucrose, the index component of Yuanzhi (a well-known Traditional Chinese Medicine or TCM), presents antidepressant effects at a molecular level, and has become a hotspot of research on new lead drug compounds.
27783048	1	12	theme	acid	68:71	arg1	class					112:116	a class	110:116	a class of natural product	110:135	Cinnamic acid sugar ester derivatives (CASEDs) are a class of natural product with one or several phenylacrylic moieties linked with the non-anomeric carbon of a glycosyl skeleton part through ester bonds.
27783048	1	12	theme	acid	68:71	arg1	CASEDs					98:103	CASEDs	98:103	CASEDs	98:103	Cinnamic acid sugar ester derivatives (CASEDs) are a class of natural product with one or several phenylacrylic moieties linked with the non-anomeric carbon of a glycosyl skeleton part through ester bonds.
27783048	1	12	theme	acid	68:71	arg1	derivatives					85:95	Cinnamic acid sugar ester derivatives	59:95	Cinnamic acid sugar ester derivatives (CASEDs)	59:104	Cinnamic acid sugar ester derivatives (CASEDs) are a class of natural product with one or several phenylacrylic moieties linked with the non-anomeric carbon of a glycosyl skeleton part through ester bonds.
27783048	3	13	attach	presents	534:541	arg1	level					581:585	a molecular level	569:585	a molecular level	569:585	In particular the compound 3',6-disinapoylsucrose, the index component of Yuanzhi (a well-known Traditional Chinese Medicine or TCM), presents antidepressant effects at a molecular level, and has become a hotspot of research on new lead drug compounds.
27783048	3	13	attach	presents	534:541	arg1	particular					403:412	particular	403:412	particular	403:412	In particular the compound 3',6-disinapoylsucrose, the index component of Yuanzhi (a well-known Traditional Chinese Medicine or TCM), presents antidepressant effects at a molecular level, and has become a hotspot of research on new lead drug compounds.
27783048	3	13	attach	presents	534:541	arg2	component					461:469	the index component	451:469	the index component of Yuanzhi (a well-known Traditional Chinese Medicine or TCM)	451:531	In particular the compound 3',6-disinapoylsucrose, the index component of Yuanzhi (a well-known Traditional Chinese Medicine or TCM), presents antidepressant effects at a molecular level, and has become a hotspot of research on new lead drug compounds.
27783048	3	13	attach	presents	534:541	arg2	compound					418:425	the compound 3',6-disinapoylsucrose, the index component of Yuanzhi (a well-known Traditional Chinese Medicine or TCM),	414:532	compound	418:425	In particular the compound 3',6-disinapoylsucrose, the index component of Yuanzhi (a well-known Traditional Chinese Medicine or TCM), presents antidepressant effects at a molecular level, and has become a hotspot of research on new lead drug compounds.
27783048	1	14	theme	ester	252:256	arg1	bonds					258:262	ester bonds	252:262	ester bonds	252:262	Cinnamic acid sugar ester derivatives (CASEDs) are a class of natural product with one or several phenylacrylic moieties linked with the non-anomeric carbon of a glycosyl skeleton part through ester bonds.
27783048	1	15	with	class	112:116	arg1	moieties					171:178	several phenylacrylic moieties	149:178	several phenylacrylic moieties	149:178	Cinnamic acid sugar ester derivatives (CASEDs) are a class of natural product with one or several phenylacrylic moieties linked with the non-anomeric carbon of a glycosyl skeleton part through ester bonds.
27783048	1	15	with	class	112:116	arg1	one					142:144	one	142:144	one	142:144	Cinnamic acid sugar ester derivatives (CASEDs) are a class of natural product with one or several phenylacrylic moieties linked with the non-anomeric carbon of a glycosyl skeleton part through ester bonds.
27783048	4	16	theme	sugar	689:693	arg1	compounds					753:761	compounds	753:761	compounds to calm the nerves and display anti-depression and neuroprotective activity	753:837	Several other similar cinnamic acid sugar ester derivatives are reported in traditional medicine as compounds to calm the nerves and display anti-depression and neuroprotective activity.
27783048	4	16	theme	sugar	689:693	arg1	derivatives					701:711	Several other similar cinnamic acid sugar ester derivatives	653:711	Several other similar cinnamic acid sugar ester derivatives	653:711	Several other similar cinnamic acid sugar ester derivatives are reported in traditional medicine as compounds to calm the nerves and display anti-depression and neuroprotective activity.
27783048	1	17	theme	sugar	73:77	arg1	class					112:116	a class	110:116	a class of natural product	110:135	Cinnamic acid sugar ester derivatives (CASEDs) are a class of natural product with one or several phenylacrylic moieties linked with the non-anomeric carbon of a glycosyl skeleton part through ester bonds.
27783048	1	17	theme	sugar	73:77	arg1	CASEDs					98:103	CASEDs	98:103	CASEDs	98:103	Cinnamic acid sugar ester derivatives (CASEDs) are a class of natural product with one or several phenylacrylic moieties linked with the non-anomeric carbon of a glycosyl skeleton part through ester bonds.
27783048	1	17	theme	sugar	73:77	arg1	derivatives					85:95	Cinnamic acid sugar ester derivatives	59:95	Cinnamic acid sugar ester derivatives (CASEDs)	59:104	Cinnamic acid sugar ester derivatives (CASEDs) are a class of natural product with one or several phenylacrylic moieties linked with the non-anomeric carbon of a glycosyl skeleton part through ester bonds.
27783048	2	18	theme	past	378:381	arg1	decades					391:397	the past several decades	374:397	the past several decades	374:397	Their notable anti-depressant and brains protective activities have made them a topic of great interest over the past several decades.
27783048	3	19	from	hotspot	605:611	arg1	compounds					642:650	new lead drug compounds	628:650	new lead drug compounds	628:650	In particular the compound 3',6-disinapoylsucrose, the index component of Yuanzhi (a well-known Traditional Chinese Medicine or TCM), presents antidepressant effects at a molecular level, and has become a hotspot of research on new lead drug compounds.
27783048	6	20	theme	cinnamic	970:977	arg1	derivatives					996:1006	cinnamic acid sugar ester derivatives	970:1006	cinnamic acid sugar ester derivatives from plants, together with a systematic discussion of their distribution, chemical structures and properties and pharmacological activities	970:1146	This overview discusses the isolation of cinnamic acid sugar ester derivatives from plants, together with a systematic discussion of their distribution, chemical structures and properties and pharmacological activities, with the hope of providing references for natural product researchers and draw attention to these interesting compounds.
27783048	6	21	theme	pharmacological	1121:1135	arg1	activities					1137:1146	pharmacological activities	1121:1146	pharmacological activities	1121:1146	This overview discusses the isolation of cinnamic acid sugar ester derivatives from plants, together with a systematic discussion of their distribution, chemical structures and properties and pharmacological activities, with the hope of providing references for natural product researchers and draw attention to these interesting compounds.
27783048	6	21	theme	pharmacological	1121:1135	arg1	plants					1013:1018	plants	1013:1018	plants	1013:1018	This overview discusses the isolation of cinnamic acid sugar ester derivatives from plants, together with a systematic discussion of their distribution, chemical structures and properties and pharmacological activities, with the hope of providing references for natural product researchers and draw attention to these interesting compounds.
27783048	1	22	theme	ester	79:83	arg1	class					112:116	a class	110:116	a class of natural product	110:135	Cinnamic acid sugar ester derivatives (CASEDs) are a class of natural product with one or several phenylacrylic moieties linked with the non-anomeric carbon of a glycosyl skeleton part through ester bonds.
27783048	1	22	theme	ester	79:83	arg1	CASEDs					98:103	CASEDs	98:103	CASEDs	98:103	Cinnamic acid sugar ester derivatives (CASEDs) are a class of natural product with one or several phenylacrylic moieties linked with the non-anomeric carbon of a glycosyl skeleton part through ester bonds.
27783048	1	22	theme	ester	79:83	arg1	derivatives					85:95	Cinnamic acid sugar ester derivatives	59:95	Cinnamic acid sugar ester derivatives (CASEDs)	59:104	Cinnamic acid sugar ester derivatives (CASEDs) are a class of natural product with one or several phenylacrylic moieties linked with the non-anomeric carbon of a glycosyl skeleton part through ester bonds.
27783048	1	23	theme	several	149:155	arg1	moieties					171:178	several phenylacrylic moieties	149:178	several phenylacrylic moieties	149:178	Cinnamic acid sugar ester derivatives (CASEDs) are a class of natural product with one or several phenylacrylic moieties linked with the non-anomeric carbon of a glycosyl skeleton part through ester bonds.
27783048	3	24	theme	well-known	485:494	arg1	Medicine					516:523	a well-known Traditional Chinese Medicine	483:523	a well-known Traditional Chinese Medicine	483:523	In particular the compound 3',6-disinapoylsucrose, the index component of Yuanzhi (a well-known Traditional Chinese Medicine or TCM), presents antidepressant effects at a molecular level, and has become a hotspot of research on new lead drug compounds.
27783048	4	25	theme	neuroprotective	814:828	arg1	activity					830:837	neuroprotective activity	814:837	neuroprotective activity	814:837	Several other similar cinnamic acid sugar ester derivatives are reported in traditional medicine as compounds to calm the nerves and display anti-depression and neuroprotective activity.
27783048	2	26	theme	protective	306:315	arg1	activities					317:326	Their notable anti-depressant and brains protective activities	265:326	Their notable anti-depressant and brains protective activities	265:326	Their notable anti-depressant and brains protective activities have made them a topic of great interest over the past several decades.
27783048	1	27	theme	phenylacrylic	157:169	arg1	moieties					171:178	several phenylacrylic moieties	149:178	several phenylacrylic moieties	149:178	Cinnamic acid sugar ester derivatives (CASEDs) are a class of natural product with one or several phenylacrylic moieties linked with the non-anomeric carbon of a glycosyl skeleton part through ester bonds.
27783048	6	28	theme	interesting	1247:1257	arg1	compounds					1259:1267	these interesting compounds	1241:1267	these interesting compounds	1241:1267	This overview discusses the isolation of cinnamic acid sugar ester derivatives from plants, together with a systematic discussion of their distribution, chemical structures and properties and pharmacological activities, with the hope of providing references for natural product researchers and draw attention to these interesting compounds.
27783048	3	29	theme	research	616:623	arg1	hotspot					605:611	a hotspot	603:611	a hotspot of research on new lead drug compounds	603:650	In particular the compound 3',6-disinapoylsucrose, the index component of Yuanzhi (a well-known Traditional Chinese Medicine or TCM), presents antidepressant effects at a molecular level, and has become a hotspot of research on new lead drug compounds.
27783048	2	30	theme	brains	299:304	arg1	activities					317:326	Their notable anti-depressant and brains protective activities	265:326	Their notable anti-depressant and brains protective activities	265:326	Their notable anti-depressant and brains protective activities have made them a topic of great interest over the past several decades.
27783048	2	31	theme	interest	360:367	arg1	topic					345:349	a topic	343:349	a topic of great interest	343:367	Their notable anti-depressant and brains protective activities have made them a topic of great interest over the past several decades.
27783048	0	32	theme	Acid	29:32	arg1	Derivatives					46:56	Cinnamic Acid Sugar Ester Derivatives	20:56	Cinnamic Acid Sugar Ester Derivatives	20:56	Naturally Occurring Cinnamic Acid Sugar Ester Derivatives.
27783048	4	33	theme	acid	684:687	arg1	compounds					753:761	compounds	753:761	compounds to calm the nerves and display anti-depression and neuroprotective activity	753:837	Several other similar cinnamic acid sugar ester derivatives are reported in traditional medicine as compounds to calm the nerves and display anti-depression and neuroprotective activity.
27783048	4	33	theme	acid	684:687	arg1	derivatives					701:711	Several other similar cinnamic acid sugar ester derivatives	653:711	Several other similar cinnamic acid sugar ester derivatives	653:711	Several other similar cinnamic acid sugar ester derivatives are reported in traditional medicine as compounds to calm the nerves and display anti-depression and neuroprotective activity.
27783048	5	34	theme	family	908:913	arg1	Polygalaceae					915:926	the family Polygalaceae	904:926	the family Polygalaceae	904:926	Interestingly, more than one third of CASEDs are distributed in the family Polygalaceae.
27783048	0	35	theme	Cinnamic	20:27	arg1	Derivatives					46:56	Cinnamic Acid Sugar Ester Derivatives	20:56	Cinnamic Acid Sugar Ester Derivatives	20:56	Naturally Occurring Cinnamic Acid Sugar Ester Derivatives.
27783048	3	36	theme	Traditional	496:506	arg1	Medicine					516:523	a well-known Traditional Chinese Medicine	483:523	a well-known Traditional Chinese Medicine	483:523	In particular the compound 3',6-disinapoylsucrose, the index component of Yuanzhi (a well-known Traditional Chinese Medicine or TCM), presents antidepressant effects at a molecular level, and has become a hotspot of research on new lead drug compounds.
27783048	2	37	theme	anti-depressant	279:293	arg1	activities					317:326	Their notable anti-depressant and brains protective activities	265:326	Their notable anti-depressant and brains protective activities	265:326	Their notable anti-depressant and brains protective activities have made them a topic of great interest over the past several decades.
27783048	1	38	theme	natural	121:127	arg1	product					129:135	natural product	121:135	natural product	121:135	Cinnamic acid sugar ester derivatives (CASEDs) are a class of natural product with one or several phenylacrylic moieties linked with the non-anomeric carbon of a glycosyl skeleton part through ester bonds.
27783048	0	39	theme	Ester	40:44	arg1	Derivatives					46:56	Cinnamic Acid Sugar Ester Derivatives	20:56	Cinnamic Acid Sugar Ester Derivatives	20:56	Naturally Occurring Cinnamic Acid Sugar Ester Derivatives.
27783048	5	40	theme	CASEDs	878:883	arg1	third					869:873	third	869:873	third	869:873	Interestingly, more than one third of CASEDs are distributed in the family Polygalaceae.
27783048	6	41	from	plants	1013:1018	arg1	isolation					957:965	the isolation	953:965	the isolation of cinnamic acid sugar ester derivatives from plants, together with a systematic discussion of their distribution, chemical structures and properties and pharmacological activities	953:1146	This overview discusses the isolation of cinnamic acid sugar ester derivatives from plants, together with a systematic discussion of their distribution, chemical structures and properties and pharmacological activities, with the hope of providing references for natural product researchers and draw attention to these interesting compounds.
27783048	6	41	from	plants	1013:1018	arg1	derivatives					996:1006	cinnamic acid sugar ester derivatives	970:1006	cinnamic acid sugar ester derivatives from plants, together with a systematic discussion of their distribution, chemical structures and properties and pharmacological activities	970:1146	This overview discusses the isolation of cinnamic acid sugar ester derivatives from plants, together with a systematic discussion of their distribution, chemical structures and properties and pharmacological activities, with the hope of providing references for natural product researchers and draw attention to these interesting compounds.
27783048	0	42	theme	Sugar	34:38	arg1	Derivatives					46:56	Cinnamic Acid Sugar Ester Derivatives	20:56	Cinnamic Acid Sugar Ester Derivatives	20:56	Naturally Occurring Cinnamic Acid Sugar Ester Derivatives.
27783048	2	43	theme	several	383:389	arg1	decades					391:397	the past several decades	374:397	the past several decades	374:397	Their notable anti-depressant and brains protective activities have made them a topic of great interest over the past several decades.
27783048	3	44	theme	antidepressant	543:556	arg1	effects					558:564	antidepressant effects	543:564	antidepressant effects	543:564	In particular the compound 3',6-disinapoylsucrose, the index component of Yuanzhi (a well-known Traditional Chinese Medicine or TCM), presents antidepressant effects at a molecular level, and has become a hotspot of research on new lead drug compounds.
27783048	4	45	theme	other	661:665	arg1	compounds					753:761	compounds	753:761	compounds to calm the nerves and display anti-depression and neuroprotective activity	753:837	Several other similar cinnamic acid sugar ester derivatives are reported in traditional medicine as compounds to calm the nerves and display anti-depression and neuroprotective activity.
27783048	4	45	theme	other	661:665	arg1	derivatives					701:711	Several other similar cinnamic acid sugar ester derivatives	653:711	Several other similar cinnamic acid sugar ester derivatives	653:711	Several other similar cinnamic acid sugar ester derivatives are reported in traditional medicine as compounds to calm the nerves and display anti-depression and neuroprotective activity.
27783048	6	46	theme	product	1199:1205	arg1	researchers					1207:1217	natural product researchers	1191:1217	natural product researchers	1191:1217	This overview discusses the isolation of cinnamic acid sugar ester derivatives from plants, together with a systematic discussion of their distribution, chemical structures and properties and pharmacological activities, with the hope of providing references for natural product researchers and draw attention to these interesting compounds.
27783048	4	47	theme	Several	653:659	arg1	compounds					753:761	compounds	753:761	compounds to calm the nerves and display anti-depression and neuroprotective activity	753:837	Several other similar cinnamic acid sugar ester derivatives are reported in traditional medicine as compounds to calm the nerves and display anti-depression and neuroprotective activity.
27783048	4	47	theme	Several	653:659	arg1	derivatives					701:711	Several other similar cinnamic acid sugar ester derivatives	653:711	Several other similar cinnamic acid sugar ester derivatives	653:711	Several other similar cinnamic acid sugar ester derivatives are reported in traditional medicine as compounds to calm the nerves and display anti-depression and neuroprotective activity.
27783048	6	48	theme	providing	1166:1174	arg1	references					1176:1185	providing references	1166:1185	providing references	1166:1185	This overview discusses the isolation of cinnamic acid sugar ester derivatives from plants, together with a systematic discussion of their distribution, chemical structures and properties and pharmacological activities, with the hope of providing references for natural product researchers and draw attention to these interesting compounds.
27783048	3	49	theme	new	628:630	arg1	compounds					642:650	new lead drug compounds	628:650	new lead drug compounds	628:650	In particular the compound 3',6-disinapoylsucrose, the index component of Yuanzhi (a well-known Traditional Chinese Medicine or TCM), presents antidepressant effects at a molecular level, and has become a hotspot of research on new lead drug compounds.
27783048	6	50	theme	natural	1191:1197	arg1	researchers					1207:1217	natural product researchers	1191:1217	natural product researchers	1191:1217	This overview discusses the isolation of cinnamic acid sugar ester derivatives from plants, together with a systematic discussion of their distribution, chemical structures and properties and pharmacological activities, with the hope of providing references for natural product researchers and draw attention to these interesting compounds.
27783048	6	51	theme	ester	990:994	arg1	derivatives					996:1006	cinnamic acid sugar ester derivatives	970:1006	cinnamic acid sugar ester derivatives from plants, together with a systematic discussion of their distribution, chemical structures and properties and pharmacological activities	970:1146	This overview discusses the isolation of cinnamic acid sugar ester derivatives from plants, together with a systematic discussion of their distribution, chemical structures and properties and pharmacological activities, with the hope of providing references for natural product researchers and draw attention to these interesting compounds.
27783048	4	52	theme	cinnamic	675:682	arg1	compounds					753:761	compounds	753:761	compounds to calm the nerves and display anti-depression and neuroprotective activity	753:837	Several other similar cinnamic acid sugar ester derivatives are reported in traditional medicine as compounds to calm the nerves and display anti-depression and neuroprotective activity.
27783048	4	52	theme	cinnamic	675:682	arg1	derivatives					701:711	Several other similar cinnamic acid sugar ester derivatives	653:711	Several other similar cinnamic acid sugar ester derivatives	653:711	Several other similar cinnamic acid sugar ester derivatives are reported in traditional medicine as compounds to calm the nerves and display anti-depression and neuroprotective activity.
27783048	6	53	theme	chemical	1082:1089	arg1	structures					1091:1100	chemical structures	1082:1100	chemical structures	1082:1100	This overview discusses the isolation of cinnamic acid sugar ester derivatives from plants, together with a systematic discussion of their distribution, chemical structures and properties and pharmacological activities, with the hope of providing references for natural product researchers and draw attention to these interesting compounds.
27783048	6	53	theme	chemical	1082:1089	arg1	plants					1013:1018	plants	1013:1018	plants	1013:1018	This overview discusses the isolation of cinnamic acid sugar ester derivatives from plants, together with a systematic discussion of their distribution, chemical structures and properties and pharmacological activities, with the hope of providing references for natural product researchers and draw attention to these interesting compounds.
27783048	6	54	theme	derivatives	996:1006	arg1	isolation					957:965	the isolation	953:965	the isolation of cinnamic acid sugar ester derivatives from plants, together with a systematic discussion of their distribution, chemical structures and properties and pharmacological activities	953:1146	This overview discusses the isolation of cinnamic acid sugar ester derivatives from plants, together with a systematic discussion of their distribution, chemical structures and properties and pharmacological activities, with the hope of providing references for natural product researchers and draw attention to these interesting compounds.
27783048	3	55	theme	Chinese	508:514	arg1	Medicine					516:523	a well-known Traditional Chinese Medicine	483:523	a well-known Traditional Chinese Medicine	483:523	In particular the compound 3',6-disinapoylsucrose, the index component of Yuanzhi (a well-known Traditional Chinese Medicine or TCM), presents antidepressant effects at a molecular level, and has become a hotspot of research on new lead drug compounds.
27783048	2	56	theme	notable	271:277	arg1	activities					317:326	Their notable anti-depressant and brains protective activities	265:326	Their notable anti-depressant and brains protective activities	265:326	Their notable anti-depressant and brains protective activities have made them a topic of great interest over the past several decades.
27783048	3	57	theme	index	455:459	arg1	component					461:469	the index component	451:469	the index component of Yuanzhi (a well-known Traditional Chinese Medicine or TCM)	451:531	In particular the compound 3',6-disinapoylsucrose, the index component of Yuanzhi (a well-known Traditional Chinese Medicine or TCM), presents antidepressant effects at a molecular level, and has become a hotspot of research on new lead drug compounds.
27783048	3	57	theme	index	455:459	arg1	compound					418:425	the compound 3',6-disinapoylsucrose, the index component of Yuanzhi (a well-known Traditional Chinese Medicine or TCM),	414:532	compound	418:425	In particular the compound 3',6-disinapoylsucrose, the index component of Yuanzhi (a well-known Traditional Chinese Medicine or TCM), presents antidepressant effects at a molecular level, and has become a hotspot of research on new lead drug compounds.
27783048	4	58	theme	similar	667:673	arg1	compounds					753:761	compounds	753:761	compounds to calm the nerves and display anti-depression and neuroprotective activity	753:837	Several other similar cinnamic acid sugar ester derivatives are reported in traditional medicine as compounds to calm the nerves and display anti-depression and neuroprotective activity.
27783048	4	58	theme	similar	667:673	arg1	derivatives					701:711	Several other similar cinnamic acid sugar ester derivatives	653:711	Several other similar cinnamic acid sugar ester derivatives	653:711	Several other similar cinnamic acid sugar ester derivatives are reported in traditional medicine as compounds to calm the nerves and display anti-depression and neuroprotective activity.
27783048	1	59	theme	non-anomeric	196:207	arg1	carbon					209:214	the non-anomeric carbon	192:214	the non-anomeric carbon of a glycosyl skeleton part through ester bonds	192:262	Cinnamic acid sugar ester derivatives (CASEDs) are a class of natural product with one or several phenylacrylic moieties linked with the non-anomeric carbon of a glycosyl skeleton part through ester bonds.
27783048	3	60	theme	molecular	571:579	arg1	level					581:585	a molecular level	569:585	a molecular level	569:585	In particular the compound 3',6-disinapoylsucrose, the index component of Yuanzhi (a well-known Traditional Chinese Medicine or TCM), presents antidepressant effects at a molecular level, and has become a hotspot of research on new lead drug compounds.
27783048	2	61	theme	great	354:358	arg1	interest					360:367	great interest	354:367	great interest	354:367	Their notable anti-depressant and brains protective activities have made them a topic of great interest over the past several decades.
27783048	6	62	theme	systematic	1037:1046	arg1	discussion					1048:1057	a systematic discussion	1035:1057	a systematic discussion of their distribution	1035:1079	This overview discusses the isolation of cinnamic acid sugar ester derivatives from plants, together with a systematic discussion of their distribution, chemical structures and properties and pharmacological activities, with the hope of providing references for natural product researchers and draw attention to these interesting compounds.
27783048	6	63	theme	sugar	984:988	arg1	derivatives					996:1006	cinnamic acid sugar ester derivatives	970:1006	cinnamic acid sugar ester derivatives from plants, together with a systematic discussion of their distribution, chemical structures and properties and pharmacological activities	970:1146	This overview discusses the isolation of cinnamic acid sugar ester derivatives from plants, together with a systematic discussion of their distribution, chemical structures and properties and pharmacological activities, with the hope of providing references for natural product researchers and draw attention to these interesting compounds.
27783048	6	64	theme	acid	979:982	arg1	derivatives					996:1006	cinnamic acid sugar ester derivatives	970:1006	cinnamic acid sugar ester derivatives from plants, together with a systematic discussion of their distribution, chemical structures and properties and pharmacological activities	970:1146	This overview discusses the isolation of cinnamic acid sugar ester derivatives from plants, together with a systematic discussion of their distribution, chemical structures and properties and pharmacological activities, with the hope of providing references for natural product researchers and draw attention to these interesting compounds.
27783048	1	65	theme	product	129:135	arg1	class					112:116	a class	110:116	a class of natural product	110:135	Cinnamic acid sugar ester derivatives (CASEDs) are a class of natural product with one or several phenylacrylic moieties linked with the non-anomeric carbon of a glycosyl skeleton part through ester bonds.
27783048	1	65	theme	product	129:135	arg1	derivatives					85:95	Cinnamic acid sugar ester derivatives	59:95	Cinnamic acid sugar ester derivatives (CASEDs)	59:104	Cinnamic acid sugar ester derivatives (CASEDs) are a class of natural product with one or several phenylacrylic moieties linked with the non-anomeric carbon of a glycosyl skeleton part through ester bonds.
27783048	1	66	theme	glycosyl	221:228	arg1	part					239:242	a glycosyl skeleton part	219:242	a glycosyl skeleton part through ester bonds	219:262	Cinnamic acid sugar ester derivatives (CASEDs) are a class of natural product with one or several phenylacrylic moieties linked with the non-anomeric carbon of a glycosyl skeleton part through ester bonds.
25799882	0	0	theme	Streptomyces	94:105	arg1	sp					107:108	Streptomyces sp	94:108	Streptomyces sp	94:108	Production of polysaccharide-based bioflocculant for the synthesis of silver nanoparticles by Streptomyces sp.
25799882	3	1	theme	green	384:388	arg1	method					390:395	A simple, cost-effective and green method	355:395	A simple, cost-effective and green method	355:395	A simple, cost-effective and green method was developed for the biosynthesis of silver nanoparticles using polysaccharides as reducing and stabilizing agents.
25799882	8	2	theme	silver	1054:1059	arg1	nanoparticles					1061:1073	The biosynthesized silver nanoparticles	1035:1073	The biosynthesized silver nanoparticles	1035:1073	The biosynthesized silver nanoparticles were characterized by UV-vis spectroscopy, XRD, FTIR, FESEM, EDXA and HRTEM.
25799882	0	3	theme	polysaccharide-based	14:33	arg1	bioflocculant					35:47	polysaccharide-based bioflocculant	14:47	polysaccharide-based bioflocculant for the synthesis of silver	14:75	Production of polysaccharide-based bioflocculant for the synthesis of silver nanoparticles by Streptomyces sp.
25799882	4	4	theme	bioflocculant	598:610	arg1	production					546:555	production	546:555	production	546:555	In this paper, we report on the production and optimization of polysaccharide-based bioflocculant for the green synthesis of silver nanoparticles by Streptomyces sp.
25799882	4	4	theme	bioflocculant	598:610	arg1	optimization					561:572	optimization	561:572	optimization	561:572	In this paper, we report on the production and optimization of polysaccharide-based bioflocculant for the green synthesis of silver nanoparticles by Streptomyces sp.
25799882	10	5	theme	bionanomaterials	1441:1456	arg1	development					1409:1419	the development	1405:1419	the development of new bactericidal bionanomaterials for wastewater treatment and biotechnological applications	1405:1515	Therefore, the biosynthesized silver nanoparticles can be extended as an alternative for the development of new bactericidal bionanomaterials for wastewater treatment and biotechnological applications.
25799882	4	6	theme	Streptomyces	663:674	arg1	sp					676:677	Streptomyces sp	663:677	Streptomyces sp	663:677	In this paper, we report on the production and optimization of polysaccharide-based bioflocculant for the green synthesis of silver nanoparticles by Streptomyces sp.
25799882	4	7	theme	polysaccharide-based	577:596	arg1	bioflocculant					598:610	polysaccharide-based bioflocculant	577:610	polysaccharide-based bioflocculant for the green synthesis of silver nanoparticles by Streptomyces sp	577:677	In this paper, we report on the production and optimization of polysaccharide-based bioflocculant for the green synthesis of silver nanoparticles by Streptomyces sp.
25799882	9	8	theme	new	1276:1278	arg1	avenue					1280:1285	a new avenue	1274:1285	a new avenue in the wastewater treatment	1274:1313	The biosynthesized silver nanoparticles revealed strong antibacterial activity in sewage water and this result could make a new avenue in the wastewater treatment.
25799882	6	9	theme	culture	712:718	arg1	conditions					720:729	culture conditions	712:729	culture conditions	712:729	Medium composition and culture conditions for polysaccharide-based bioflocculants were statistically optimized by response surface methodology (RSM).
25799882	10	10	theme	biotechnological	1487:1502	arg1	applications					1504:1515	biotechnological applications	1487:1515	biotechnological applications	1487:1515	Therefore, the biosynthesized silver nanoparticles can be extended as an alternative for the development of new bactericidal bionanomaterials for wastewater treatment and biotechnological applications.
25799882	6	11	theme	Medium	689:694	arg1	composition					696:706	Medium composition	689:706	Medium composition	689:706	Medium composition and culture conditions for polysaccharide-based bioflocculants were statistically optimized by response surface methodology (RSM).
25799882	8	12	theme	UV-vis	1097:1102	arg1	spectroscopy					1104:1115	UV-vis spectroscopy	1097:1115	UV-vis spectroscopy	1097:1115	The biosynthesized silver nanoparticles were characterized by UV-vis spectroscopy, XRD, FTIR, FESEM, EDXA and HRTEM.
25799882	9	13	theme	antibacterial	1208:1220	arg1	activity					1222:1229	strong antibacterial activity	1201:1229	strong antibacterial activity in sewage water	1201:1245	The biosynthesized silver nanoparticles revealed strong antibacterial activity in sewage water and this result could make a new avenue in the wastewater treatment.
25799882	7	14	theme	bioflocculant	843:855	arg1	production					857:866	The bioflocculant production	839:866	The bioflocculant production	839:866	The bioflocculant production was statistically optimized with most significant factors, namely palm jaggery (18.73g/L), yeast extract (2.07g/L), K2HPO4 (3.74g/L) and NaCl (0.38g/L), respectively.
25799882	10	15	theme	applications	1504:1515	arg1	development					1409:1419	the development	1405:1419	the development of new bactericidal bionanomaterials for wastewater treatment and biotechnological applications	1405:1515	Therefore, the biosynthesized silver nanoparticles can be extended as an alternative for the development of new bactericidal bionanomaterials for wastewater treatment and biotechnological applications.
25799882	9	16	theme	biosynthesized	1156:1169	arg1	nanoparticles					1178:1190	The biosynthesized silver nanoparticles	1152:1190	The biosynthesized silver nanoparticles	1152:1190	The biosynthesized silver nanoparticles revealed strong antibacterial activity in sewage water and this result could make a new avenue in the wastewater treatment.
25799882	9	17	theme	wastewater	1294:1303	arg1	treatment					1305:1313	the wastewater treatment	1290:1313	the wastewater treatment	1290:1313	The biosynthesized silver nanoparticles revealed strong antibacterial activity in sewage water and this result could make a new avenue in the wastewater treatment.
25799882	9	18	theme	silver	1171:1176	arg1	nanoparticles					1178:1190	The biosynthesized silver nanoparticles	1152:1190	The biosynthesized silver nanoparticles	1152:1190	The biosynthesized silver nanoparticles revealed strong antibacterial activity in sewage water and this result could make a new avenue in the wastewater treatment.
25799882	1	19	from	attention	175:183	arg1	years					195:199	recent years	188:199	recent years	188:199	Polysaccharide-based bioflocculants have attracted considerable attention in recent years due to their biodegradable, harmless and negligible secondary pollution.
25799882	1	20	theme	biodegradable	214:226	arg1	pollution					263:271	their biodegradable, harmless and negligible secondary pollution	208:271	their biodegradable, harmless and negligible secondary pollution	208:271	Polysaccharide-based bioflocculants have attracted considerable attention in recent years due to their biodegradable, harmless and negligible secondary pollution.
25799882	4	21	theme	silver	639:644	arg1	nanoparticles					646:658	silver nanoparticles	639:658	silver nanoparticles	639:658	In this paper, we report on the production and optimization of polysaccharide-based bioflocculant for the green synthesis of silver nanoparticles by Streptomyces sp.
25799882	7	22	theme	yeast	959:963	arg1	2.07g/L					974:980	2.07g/L	974:980	2.07g/L	974:980	The bioflocculant production was statistically optimized with most significant factors, namely palm jaggery (18.73g/L), yeast extract (2.07g/L), K2HPO4 (3.74g/L) and NaCl (0.38g/L), respectively.
25799882	7	22	theme	yeast	959:963	arg1	extract					965:971	yeast extract	959:971	yeast extract (2.07g/L)	959:981	The bioflocculant production was statistically optimized with most significant factors, namely palm jaggery (18.73g/L), yeast extract (2.07g/L), K2HPO4 (3.74g/L) and NaCl (0.38g/L), respectively.
25799882	7	22	theme	yeast	959:963	arg1	factors					918:924	most significant factors	901:924	most significant factors	901:924	The bioflocculant production was statistically optimized with most significant factors, namely palm jaggery (18.73g/L), yeast extract (2.07g/L), K2HPO4 (3.74g/L) and NaCl (0.38g/L), respectively.
25799882	10	23	theme	wastewater	1462:1471	arg1	treatment					1473:1481	wastewater treatment	1462:1481	wastewater treatment	1462:1481	Therefore, the biosynthesized silver nanoparticles can be extended as an alternative for the development of new bactericidal bionanomaterials for wastewater treatment and biotechnological applications.
25799882	3	24	theme	silver	435:440	arg1	nanoparticles					442:454	silver nanoparticles	435:454	silver nanoparticles using polysaccharides as reducing and stabilizing agents	435:511	A simple, cost-effective and green method was developed for the biosynthesis of silver nanoparticles using polysaccharides as reducing and stabilizing agents.
25799882	4	25	dep	production	546:555	arg1	the					542:544	the	542:544	the	542:544	In this paper, we report on the production and optimization of polysaccharide-based bioflocculant for the green synthesis of silver nanoparticles by Streptomyces sp.
25799882	1	26	theme	Polysaccharide-based	111:130	arg1	bioflocculants					132:145	Polysaccharide-based bioflocculants	111:145	Polysaccharide-based bioflocculants	111:145	Polysaccharide-based bioflocculants have attracted considerable attention in recent years due to their biodegradable, harmless and negligible secondary pollution.
25799882	6	27	theme	surface	812:818	arg1	RSM					833:835	RSM	833:835	RSM	833:835	Medium composition and culture conditions for polysaccharide-based bioflocculants were statistically optimized by response surface methodology (RSM).
25799882	6	27	theme	surface	812:818	arg1	methodology					820:830	response surface methodology	803:830	response surface methodology (RSM)	803:836	Medium composition and culture conditions for polysaccharide-based bioflocculants were statistically optimized by response surface methodology (RSM).
25799882	1	28	theme	harmless	229:236	arg1	pollution					263:271	their biodegradable, harmless and negligible secondary pollution	208:271	their biodegradable, harmless and negligible secondary pollution	208:271	Polysaccharide-based bioflocculants have attracted considerable attention in recent years due to their biodegradable, harmless and negligible secondary pollution.
25799882	3	29	theme	nanoparticles	442:454	arg1	biosynthesis					419:430	the biosynthesis	415:430	the biosynthesis of silver nanoparticles using polysaccharides as reducing and stabilizing agents	415:511	A simple, cost-effective and green method was developed for the biosynthesis of silver nanoparticles using polysaccharides as reducing and stabilizing agents.
25799882	7	30	theme	palm	934:937	arg1	jaggery					939:945	palm jaggery	934:945	palm jaggery (18.73g/L)	934:956	The bioflocculant production was statistically optimized with most significant factors, namely palm jaggery (18.73g/L), yeast extract (2.07g/L), K2HPO4 (3.74g/L) and NaCl (0.38g/L), respectively.
25799882	7	30	theme	palm	934:937	arg1	factors					918:924	most significant factors	901:924	most significant factors	901:924	The bioflocculant production was statistically optimized with most significant factors, namely palm jaggery (18.73g/L), yeast extract (2.07g/L), K2HPO4 (3.74g/L) and NaCl (0.38g/L), respectively.
25799882	7	30	theme	palm	934:937	arg1	18.73g/L					948:955	18.73g/L	948:955	18.73g/L	948:955	The bioflocculant production was statistically optimized with most significant factors, namely palm jaggery (18.73g/L), yeast extract (2.07g/L), K2HPO4 (3.74g/L) and NaCl (0.38g/L), respectively.
25799882	6	31	theme	response	803:810	arg1	RSM					833:835	RSM	833:835	RSM	833:835	Medium composition and culture conditions for polysaccharide-based bioflocculants were statistically optimized by response surface methodology (RSM).
25799882	6	31	theme	response	803:810	arg1	methodology					820:830	response surface methodology	803:830	response surface methodology (RSM)	803:836	Medium composition and culture conditions for polysaccharide-based bioflocculants were statistically optimized by response surface methodology (RSM).
25799882	4	32	theme	nanoparticles	646:658	arg1	synthesis					626:634	the green synthesis	616:634	the green synthesis of silver nanoparticles by Streptomyces sp	616:677	In this paper, we report on the production and optimization of polysaccharide-based bioflocculant for the green synthesis of silver nanoparticles by Streptomyces sp.
25799882	1	33	theme	negligible	242:251	arg1	pollution					263:271	their biodegradable, harmless and negligible secondary pollution	208:271	their biodegradable, harmless and negligible secondary pollution	208:271	Polysaccharide-based bioflocculants have attracted considerable attention in recent years due to their biodegradable, harmless and negligible secondary pollution.
25799882	3	34	theme	stabilizing	494:504	arg1	agents					506:511	reducing and stabilizing agents	481:511	reducing and stabilizing agents	481:511	A simple, cost-effective and green method was developed for the biosynthesis of silver nanoparticles using polysaccharides as reducing and stabilizing agents.
25799882	3	34	theme	stabilizing	494:504	arg1	polysaccharides					462:476	polysaccharides	462:476	polysaccharides	462:476	A simple, cost-effective and green method was developed for the biosynthesis of silver nanoparticles using polysaccharides as reducing and stabilizing agents.
25799882	0	35	theme	bioflocculant	35:47	arg1	Production					0:9	Production	0:9	Production of polysaccharide-based bioflocculant for the synthesis of silver	0:75	Production of polysaccharide-based bioflocculant for the synthesis of silver nanoparticles by Streptomyces sp.
25799882	1	36	theme	secondary	253:261	arg1	pollution					263:271	their biodegradable, harmless and negligible secondary pollution	208:271	their biodegradable, harmless and negligible secondary pollution	208:271	Polysaccharide-based bioflocculants have attracted considerable attention in recent years due to their biodegradable, harmless and negligible secondary pollution.
25799882	3	37	theme	cost-effective	365:378	arg1	method					390:395	A simple, cost-effective and green method	355:395	A simple, cost-effective and green method	355:395	A simple, cost-effective and green method was developed for the biosynthesis of silver nanoparticles using polysaccharides as reducing and stabilizing agents.
25799882	9	38	theme	sewage	1234:1239	arg1	water					1241:1245	sewage water	1234:1245	sewage water	1234:1245	The biosynthesized silver nanoparticles revealed strong antibacterial activity in sewage water and this result could make a new avenue in the wastewater treatment.
25799882	3	39	theme	reducing	481:488	arg1	agents					506:511	reducing and stabilizing agents	481:511	reducing and stabilizing agents	481:511	A simple, cost-effective and green method was developed for the biosynthesis of silver nanoparticles using polysaccharides as reducing and stabilizing agents.
25799882	3	39	theme	reducing	481:488	arg1	polysaccharides					462:476	polysaccharides	462:476	polysaccharides	462:476	A simple, cost-effective and green method was developed for the biosynthesis of silver nanoparticles using polysaccharides as reducing and stabilizing agents.
25799882	7	40	theme	significant	906:916	arg1	jaggery					939:945	palm jaggery	934:945	palm jaggery (18.73g/L)	934:956	The bioflocculant production was statistically optimized with most significant factors, namely palm jaggery (18.73g/L), yeast extract (2.07g/L), K2HPO4 (3.74g/L) and NaCl (0.38g/L), respectively.
25799882	7	40	theme	significant	906:916	arg1	K2HPO4					984:989	K2HPO4	984:989	K2HPO4 (3.74g/L)	984:999	The bioflocculant production was statistically optimized with most significant factors, namely palm jaggery (18.73g/L), yeast extract (2.07g/L), K2HPO4 (3.74g/L) and NaCl (0.38g/L), respectively.
25799882	7	40	theme	significant	906:916	arg1	extract					965:971	yeast extract	959:971	yeast extract (2.07g/L)	959:981	The bioflocculant production was statistically optimized with most significant factors, namely palm jaggery (18.73g/L), yeast extract (2.07g/L), K2HPO4 (3.74g/L) and NaCl (0.38g/L), respectively.
25799882	7	40	theme	significant	906:916	arg1	factors					918:924	most significant factors	901:924	most significant factors	901:924	The bioflocculant production was statistically optimized with most significant factors, namely palm jaggery (18.73g/L), yeast extract (2.07g/L), K2HPO4 (3.74g/L) and NaCl (0.38g/L), respectively.
25799882	7	40	theme	significant	906:916	arg1	NaCl					1005:1008	NaCl	1005:1008	NaCl (0.38g/L)	1005:1018	The bioflocculant production was statistically optimized with most significant factors, namely palm jaggery (18.73g/L), yeast extract (2.07g/L), K2HPO4 (3.74g/L) and NaCl (0.38g/L), respectively.
25799882	10	41	theme	bactericidal	1428:1439	arg1	bionanomaterials					1441:1456	new bactericidal bionanomaterials	1424:1456	new bactericidal bionanomaterials for wastewater treatment	1424:1481	Therefore, the biosynthesized silver nanoparticles can be extended as an alternative for the development of new bactericidal bionanomaterials for wastewater treatment and biotechnological applications.
25799882	10	42	theme	biosynthesized	1331:1344	arg1	nanoparticles					1353:1365	the biosynthesized silver nanoparticles	1327:1365	the biosynthesized silver nanoparticles	1327:1365	Therefore, the biosynthesized silver nanoparticles can be extended as an alternative for the development of new bactericidal bionanomaterials for wastewater treatment and biotechnological applications.
25799882	10	42	theme	biosynthesized	1331:1344	arg1	alternative					1389:1399	an alternative	1386:1399	an alternative for the development of new bactericidal bionanomaterials for wastewater treatment and biotechnological applications	1386:1515	Therefore, the biosynthesized silver nanoparticles can be extended as an alternative for the development of new bactericidal bionanomaterials for wastewater treatment and biotechnological applications.
25799882	4	43	theme	green	620:624	arg1	synthesis					626:634	the green synthesis	616:634	the green synthesis of silver nanoparticles by Streptomyces sp	616:677	In this paper, we report on the production and optimization of polysaccharide-based bioflocculant for the green synthesis of silver nanoparticles by Streptomyces sp.
25799882	3	44	theme	simple	357:362	arg1	method					390:395	A simple, cost-effective and green method	355:395	A simple, cost-effective and green method	355:395	A simple, cost-effective and green method was developed for the biosynthesis of silver nanoparticles using polysaccharides as reducing and stabilizing agents.
25799882	9	45	from	activity	1222:1229	arg1	water					1241:1245	sewage water	1234:1245	sewage water	1234:1245	The biosynthesized silver nanoparticles revealed strong antibacterial activity in sewage water and this result could make a new avenue in the wastewater treatment.
25799882	1	46	theme	considerable	162:173	arg1	attention					175:183	considerable attention	162:183	considerable attention in recent years due to their biodegradable, harmless and negligible secondary pollution	162:271	Polysaccharide-based bioflocculants have attracted considerable attention in recent years due to their biodegradable, harmless and negligible secondary pollution.
25799882	10	47	theme	new	1424:1426	arg1	bionanomaterials					1441:1456	new bactericidal bionanomaterials	1424:1456	new bactericidal bionanomaterials for wastewater treatment	1424:1481	Therefore, the biosynthesized silver nanoparticles can be extended as an alternative for the development of new bactericidal bionanomaterials for wastewater treatment and biotechnological applications.
25799882	8	48	theme	biosynthesized	1039:1052	arg1	nanoparticles					1061:1073	The biosynthesized silver nanoparticles	1035:1073	The biosynthesized silver nanoparticles	1035:1073	The biosynthesized silver nanoparticles were characterized by UV-vis spectroscopy, XRD, FTIR, FESEM, EDXA and HRTEM.
25799882	9	49	theme	strong	1201:1206	arg1	activity					1222:1229	strong antibacterial activity	1201:1229	strong antibacterial activity in sewage water	1201:1245	The biosynthesized silver nanoparticles revealed strong antibacterial activity in sewage water and this result could make a new avenue in the wastewater treatment.
25799882	10	50	theme	silver	1346:1351	arg1	nanoparticles					1353:1365	the biosynthesized silver nanoparticles	1327:1365	the biosynthesized silver nanoparticles	1327:1365	Therefore, the biosynthesized silver nanoparticles can be extended as an alternative for the development of new bactericidal bionanomaterials for wastewater treatment and biotechnological applications.
25799882	10	50	theme	silver	1346:1351	arg1	alternative					1389:1399	an alternative	1386:1399	an alternative for the development of new bactericidal bionanomaterials for wastewater treatment and biotechnological applications	1386:1515	Therefore, the biosynthesized silver nanoparticles can be extended as an alternative for the development of new bactericidal bionanomaterials for wastewater treatment and biotechnological applications.
25799882	2	51	theme	macromolecular	301:314	arg1	Bioflocculants					274:287	Bioflocculants	274:287	Bioflocculants	274:287	Bioflocculants are organic macromolecular substances secreted by microorganisms.
25799882	2	51	theme	macromolecular	301:314	arg1	substances					316:325	organic macromolecular substances	293:325	organic macromolecular substances secreted by microorganisms	293:352	Bioflocculants are organic macromolecular substances secreted by microorganisms.
25799882	9	52	from	avenue	1280:1285	arg1	treatment					1305:1313	the wastewater treatment	1290:1313	the wastewater treatment	1290:1313	The biosynthesized silver nanoparticles revealed strong antibacterial activity in sewage water and this result could make a new avenue in the wastewater treatment.
25799882	0	53	theme	silver	70:75	arg1	synthesis					57:65	the synthesis	53:65	the synthesis of silver	53:75	Production of polysaccharide-based bioflocculant for the synthesis of silver nanoparticles by Streptomyces sp.
25799882	2	54	theme	organic	293:299	arg1	Bioflocculants					274:287	Bioflocculants	274:287	Bioflocculants	274:287	Bioflocculants are organic macromolecular substances secreted by microorganisms.
25799882	2	54	theme	organic	293:299	arg1	substances					316:325	organic macromolecular substances	293:325	organic macromolecular substances secreted by microorganisms	293:352	Bioflocculants are organic macromolecular substances secreted by microorganisms.
25799882	1	55	theme	recent	188:193	arg1	years					195:199	recent years	188:199	recent years	188:199	Polysaccharide-based bioflocculants have attracted considerable attention in recent years due to their biodegradable, harmless and negligible secondary pollution.
25799882	6	56	theme	polysaccharide-based	735:754	arg1	bioflocculants					756:769	polysaccharide-based bioflocculants	735:769	polysaccharide-based bioflocculants	735:769	Medium composition and culture conditions for polysaccharide-based bioflocculants were statistically optimized by response surface methodology (RSM).
25629585	9	0	theme	proteome	1615:1622	arg1	set					1629:1631	the HeLa cell proteome data set	1601:1631	the HeLa cell proteome data set	1601:1631	In the HeLa cell proteome data set, MAGIC processed over a thousand MS(2) spectra in 3 min on a PC and reported 36 glycopeptides from 26 glycoproteins.
25629585	10	1	theme	N-glycosylation-free	1818:1837	arg1	set					1861:1863	the N-glycosylation-free Escherichia coli data set	1814:1863	the N-glycosylation-free Escherichia coli data set	1814:1863	Finally, a remarkable false discovery rate of 0 was achieved on the N-glycosylation-free Escherichia coli data set.
25629585	8	2	from	HRP	1544:1546	arg1	glycopeptides					1555:1567	16 glycopeptides	1552:1567	16 glycopeptides from the mixture via Mascot	1552:1595	For the model glycoprotein horseradish peroxidase (HRP) and a 5-glycoprotein mixture, a 2- to 31-fold increase in the relative intensities of the peptide fragments was achieved, which led to the identification of 7 tryptic glycopeptides from HRP and 16 glycopeptides from the mixture via Mascot.
25629585	8	2	from	HRP	1544:1546	arg1	glycopeptides					1525:1537	7 tryptic glycopeptides	1515:1537	7 tryptic glycopeptides from HRP	1515:1546	For the model glycoprotein horseradish peroxidase (HRP) and a 5-glycoprotein mixture, a 2- to 31-fold increase in the relative intensities of the peptide fragments was achieved, which led to the identification of 7 tryptic glycopeptides from HRP and 16 glycopeptides from the mixture via Mascot.
25629585	8	2	from	HRP	1544:1546	arg1	identification					1497:1510	the identification	1493:1510	the identification of 7 tryptic glycopeptides from HRP and 16 glycopeptides from the mixture via Mascot	1493:1595	For the model glycoprotein horseradish peroxidase (HRP) and a 5-glycoprotein mixture, a 2- to 31-fold increase in the relative intensities of the peptide fragments was achieved, which led to the identification of 7 tryptic glycopeptides from HRP and 16 glycopeptides from the mixture via Mascot.
25629585	4	3	from	present	731:737	arg1	sample					746:751	the sample	742:751	the sample	742:751	The identification of the Y1 (peptideY0 + GlcNAc) ion is critical for the correct analysis of unknown glycoproteins, especially without prior knowledge of the proteins and glycans present in the sample.
25629585	3	4	link	N-linked	489:496	arg1	spectra					542:548	intact N-linked glycopeptide collision-induced-dissociation spectra	482:548	intact N-linked glycopeptide collision-induced-dissociation spectra	482:548	In this paper, we present a mass spectrometry-based automated glycopeptide identification platform (MAGIC) to identify peptide sequences and glycan compositions directly from intact N-linked glycopeptide collision-induced-dissociation spectra.
25629585	6	5	theme	peptide	1181:1187	arg1	m/z					1189:1191	the naked peptide m/z	1171:1191	the naked peptide m/z	1171:1191	To facilitate the subsequent peptide sequence identification by common database search engines, MAGIC generates in silico spectra by overwriting the original precursor with the naked peptide m/z and removing all of the glycan-related ions.
25629585	11	6	dep	http	1888:1891	arg1	//ms.iis.sinica.edu.tw/COmics/Software_MAGIC.html					1893:1941	//ms.iis.sinica.edu.tw/COmics/Software_MAGIC.html	1893:1941	http://ms.iis.sinica.edu.tw/COmics/Software_MAGIC.html	1888:1941	MAGIC is available at http://ms.iis.sinica.edu.tw/COmics/Software_MAGIC.html .
25629585	5	7	link	N-linked	974:981	arg1	glycopeptides					983:995	N-linked glycopeptides	974:995	N-linked glycopeptides	974:995	To ensure accurate Y1-ion assignment, we propose a novel algorithm called Trident that detects a triplet pattern corresponding to [Y0, Y1, Y2] or [Y0-NH3, Y0, Y1] from the fragmentation of the common trimannosyl core of N-linked glycopeptides.
25629585	9	8	theme	thousand	1657:1664	arg1	2					1669:1669	2	1669:1669	2	1669:1669	In the HeLa cell proteome data set, MAGIC processed over a thousand MS(2) spectra in 3 min on a PC and reported 36 glycopeptides from 26 glycoproteins.
25629585	9	8	theme	thousand	1657:1664	arg1	MS					1666:1667	a thousand MS	1655:1667	a thousand MS(2) spectra	1655:1678	In the HeLa cell proteome data set, MAGIC processed over a thousand MS(2) spectra in 3 min on a PC and reported 36 glycopeptides from 26 glycoproteins.
25629585	0	9	link	N-linked	20:27	arg1	tool					57:60	an automated N-linked glycoprotein identification tool	7:60	MAGIC: an automated N-linked glycoprotein identification tool	0:60	MAGIC: an automated N-linked glycoprotein identification tool using a Y1-ion pattern matching algorithm and in silico MS² approach.
25629585	2	10	gly	glycopeptide	258:269	arg2	glycopeptide					258:269	intact glycopeptide spectra	251:277	intact glycopeptide spectra	251:277	Interpretation of intact glycopeptide spectra is crucial but challenging.
25629585	11	11	from	http	1888:1891	arg1	available					1875:1883	available	1875:1883	available	1875:1883	MAGIC is available at http://ms.iis.sinica.edu.tw/COmics/Software_MAGIC.html .
25629585	8	12	from	mixture	1578:1584	arg1	glycopeptides					1555:1567	16 glycopeptides	1552:1567	16 glycopeptides from the mixture via Mascot	1552:1595	For the model glycoprotein horseradish peroxidase (HRP) and a 5-glycoprotein mixture, a 2- to 31-fold increase in the relative intensities of the peptide fragments was achieved, which led to the identification of 7 tryptic glycopeptides from HRP and 16 glycopeptides from the mixture via Mascot.
25629585	8	12	from	mixture	1578:1584	arg1	glycopeptides					1525:1537	7 tryptic glycopeptides	1515:1537	7 tryptic glycopeptides from HRP	1515:1546	For the model glycoprotein horseradish peroxidase (HRP) and a 5-glycoprotein mixture, a 2- to 31-fold increase in the relative intensities of the peptide fragments was achieved, which led to the identification of 7 tryptic glycopeptides from HRP and 16 glycopeptides from the mixture via Mascot.
25629585	8	12	from	mixture	1578:1584	arg1	identification					1497:1510	the identification	1493:1510	the identification of 7 tryptic glycopeptides from HRP and 16 glycopeptides from the mixture via Mascot	1493:1595	For the model glycoprotein horseradish peroxidase (HRP) and a 5-glycoprotein mixture, a 2- to 31-fold increase in the relative intensities of the peptide fragments was achieved, which led to the identification of 7 tryptic glycopeptides from HRP and 16 glycopeptides from the mixture via Mascot.
25629585	5	13	theme	triplet	851:857	arg1	pattern					859:865	a triplet pattern	849:865	a triplet pattern corresponding to [Y0, Y1, Y2] or [Y0-NH3, Y0, Y1] from the fragmentation of the common trimannosyl core of N-linked glycopeptides	849:995	To ensure accurate Y1-ion assignment, we propose a novel algorithm called Trident that detects a triplet pattern corresponding to [Y0, Y1, Y2] or [Y0-NH3, Y0, Y1] from the fragmentation of the common trimannosyl core of N-linked glycopeptides.
25629585	8	14	theme	fragments	1456:1464	arg1	intensities					1429:1439	the relative intensities	1416:1439	the relative intensities of the peptide fragments	1416:1464	For the model glycoprotein horseradish peroxidase (HRP) and a 5-glycoprotein mixture, a 2- to 31-fold increase in the relative intensities of the peptide fragments was achieved, which led to the identification of 7 tryptic glycopeptides from HRP and 16 glycopeptides from the mixture via Mascot.
25629585	2	15	theme	spectra	271:277	arg1	Interpretation					233:246	Interpretation	233:246	Interpretation of intact glycopeptide spectra	233:277	Interpretation of intact glycopeptide spectra is crucial but challenging.
25629585	6	16	theme	subsequent	1016:1025	arg1	identification					1044:1057	the subsequent peptide sequence identification	1012:1057	the subsequent peptide sequence identification by common database search engines	1012:1091	To facilitate the subsequent peptide sequence identification by common database search engines, MAGIC generates in silico spectra by overwriting the original precursor with the naked peptide m/z and removing all of the glycan-related ions.
25629585	8	17	theme	glycoprotein	1316:1327	arg1	HRP					1353:1355	HRP	1353:1355	HRP	1353:1355	For the model glycoprotein horseradish peroxidase (HRP) and a 5-glycoprotein mixture, a 2- to 31-fold increase in the relative intensities of the peptide fragments was achieved, which led to the identification of 7 tryptic glycopeptides from HRP and 16 glycopeptides from the mixture via Mascot.
25629585	8	17	theme	glycoprotein	1316:1327	arg1	peroxidase					1341:1350	the model glycoprotein horseradish peroxidase	1306:1350	the model glycoprotein horseradish peroxidase (HRP)	1306:1356	For the model glycoprotein horseradish peroxidase (HRP) and a 5-glycoprotein mixture, a 2- to 31-fold increase in the relative intensities of the peptide fragments was achieved, which led to the identification of 7 tryptic glycopeptides from HRP and 16 glycopeptides from the mixture via Mascot.
25629585	2	18	theme	intact	251:256	arg1	spectra					271:277	intact glycopeptide spectra	251:277	intact glycopeptide spectra	251:277	Interpretation of intact glycopeptide spectra is crucial but challenging.
25629585	4	19	dep	proteins	710:717	arg1	the					706:708	the	706:708	the	706:708	The identification of the Y1 (peptideY0 + GlcNAc) ion is critical for the correct analysis of unknown glycoproteins, especially without prior knowledge of the proteins and glycans present in the sample.
25629585	3	20	dep	platform	397:404	arg1	MAGIC					407:411	MAGIC	407:411	MAGIC	407:411	In this paper, we present a mass spectrometry-based automated glycopeptide identification platform (MAGIC) to identify peptide sequences and glycan compositions directly from intact N-linked glycopeptide collision-induced-dissociation spectra.
25629585	0	21	theme	Y1-ion	70:75	arg1	pattern					77:83	a Y1-ion pattern	68:83	a Y1-ion pattern matching algorithm and in silico MS² approach	68:129	MAGIC: an automated N-linked glycoprotein identification tool using a Y1-ion pattern matching algorithm and in silico MS² approach.
25629585	3	22	theme	glycopeptide	369:380	arg1	platform					397:404	a mass spectrometry-based automated glycopeptide identification platform	333:404	a mass spectrometry-based automated glycopeptide identification platform (MAGIC) to identify peptide sequences and glycan compositions directly from intact N-linked glycopeptide collision-induced-dissociation spectra	333:548	In this paper, we present a mass spectrometry-based automated glycopeptide identification platform (MAGIC) to identify peptide sequences and glycan compositions directly from intact N-linked glycopeptide collision-induced-dissociation spectra.
25629585	5	23	theme	Y1-ion	773:778	arg1	assignment					780:789	accurate Y1-ion assignment	764:789	accurate Y1-ion assignment	764:789	To ensure accurate Y1-ion assignment, we propose a novel algorithm called Trident that detects a triplet pattern corresponding to [Y0, Y1, Y2] or [Y0-NH3, Y0, Y1] from the fragmentation of the common trimannosyl core of N-linked glycopeptides.
25629585	4	24	gly	glycoproteins	653:665	arg1	glycoproteins					653:665	unknown glycoproteins	645:665	unknown glycoproteins	645:665	The identification of the Y1 (peptideY0 + GlcNAc) ion is critical for the correct analysis of unknown glycoproteins, especially without prior knowledge of the proteins and glycans present in the sample.
25629585	7	25	dep	compositions	1273:1284	arg1	them					1296:1299	them	1296:1299	them	1296:1299	Finally, MAGIC computes the glycan compositions and ranks them.
25629585	3	26	theme	spectrometry-based	340:357	arg1	platform					397:404	a mass spectrometry-based automated glycopeptide identification platform	333:404	a mass spectrometry-based automated glycopeptide identification platform (MAGIC) to identify peptide sequences and glycan compositions directly from intact N-linked glycopeptide collision-induced-dissociation spectra	333:548	In this paper, we present a mass spectrometry-based automated glycopeptide identification platform (MAGIC) to identify peptide sequences and glycan compositions directly from intact N-linked glycopeptide collision-induced-dissociation spectra.
25629585	4	27	dep	ion	601:603	arg1	GlcNAc					593:598	GlcNAc	593:598	GlcNAc	593:598	The identification of the Y1 (peptideY0 + GlcNAc) ion is critical for the correct analysis of unknown glycoproteins, especially without prior knowledge of the proteins and glycans present in the sample.
25629585	4	27	dep	ion	601:603	arg1	peptideY0					581:589	peptideY0	581:589	peptideY0	581:589	The identification of the Y1 (peptideY0 + GlcNAc) ion is critical for the correct analysis of unknown glycoproteins, especially without prior knowledge of the proteins and glycans present in the sample.
25629585	5	28	gly	glycopeptides	983:995	arg2	glycopeptides					983:995	N-linked glycopeptides	974:995	N-linked glycopeptides	974:995	To ensure accurate Y1-ion assignment, we propose a novel algorithm called Trident that detects a triplet pattern corresponding to [Y0, Y1, Y2] or [Y0-NH3, Y0, Y1] from the fragmentation of the common trimannosyl core of N-linked glycopeptides.
25629585	8	29	theme	5-glycoprotein	1364:1377	arg1	mixture					1379:1385	a 5-glycoprotein mixture	1362:1385	a 5-glycoprotein mixture	1362:1385	For the model glycoprotein horseradish peroxidase (HRP) and a 5-glycoprotein mixture, a 2- to 31-fold increase in the relative intensities of the peptide fragments was achieved, which led to the identification of 7 tryptic glycopeptides from HRP and 16 glycopeptides from the mixture via Mascot.
25629585	8	30	gly	glycopeptides	1555:1567	arg1	HRP					1544:1546	HRP	1544:1546	HRP	1544:1546	For the model glycoprotein horseradish peroxidase (HRP) and a 5-glycoprotein mixture, a 2- to 31-fold increase in the relative intensities of the peptide fragments was achieved, which led to the identification of 7 tryptic glycopeptides from HRP and 16 glycopeptides from the mixture via Mascot.
25629585	8	30	gly	glycopeptides	1555:1567	arg2	glycopeptides					1555:1567	16 glycopeptides	1552:1567	16 glycopeptides from the mixture via Mascot	1552:1595	For the model glycoprotein horseradish peroxidase (HRP) and a 5-glycoprotein mixture, a 2- to 31-fold increase in the relative intensities of the peptide fragments was achieved, which led to the identification of 7 tryptic glycopeptides from HRP and 16 glycopeptides from the mixture via Mascot.
25629585	8	30	gly	glycopeptides	1555:1567	arg1	mixture					1578:1584	the mixture	1574:1584	the mixture via Mascot	1574:1595	For the model glycoprotein horseradish peroxidase (HRP) and a 5-glycoprotein mixture, a 2- to 31-fold increase in the relative intensities of the peptide fragments was achieved, which led to the identification of 7 tryptic glycopeptides from HRP and 16 glycopeptides from the mixture via Mascot.
25629585	6	31	theme	database	1069:1076	arg1	engines					1085:1091	common database search engines	1062:1091	common database search engines	1062:1091	To facilitate the subsequent peptide sequence identification by common database search engines, MAGIC generates in silico spectra by overwriting the original precursor with the naked peptide m/z and removing all of the glycan-related ions.
25629585	8	32	theme	glycopeptides	1525:1537	arg1	identification					1497:1510	the identification	1493:1510	the identification of 7 tryptic glycopeptides from HRP and 16 glycopeptides from the mixture via Mascot	1493:1595	For the model glycoprotein horseradish peroxidase (HRP) and a 5-glycoprotein mixture, a 2- to 31-fold increase in the relative intensities of the peptide fragments was achieved, which led to the identification of 7 tryptic glycopeptides from HRP and 16 glycopeptides from the mixture via Mascot.
25629585	3	33	gly	glycopeptide	498:509	arg2	glycopeptide					498:509	intact N-linked glycopeptide collision-induced-dissociation spectra	482:548	intact N-linked glycopeptide collision-induced-dissociation spectra	482:548	In this paper, we present a mass spectrometry-based automated glycopeptide identification platform (MAGIC) to identify peptide sequences and glycan compositions directly from intact N-linked glycopeptide collision-induced-dissociation spectra.
25629585	3	34	theme	N-linked	489:496	arg1	spectra					542:548	intact N-linked glycopeptide collision-induced-dissociation spectra	482:548	intact N-linked glycopeptide collision-induced-dissociation spectra	482:548	In this paper, we present a mass spectrometry-based automated glycopeptide identification platform (MAGIC) to identify peptide sequences and glycan compositions directly from intact N-linked glycopeptide collision-induced-dissociation spectra.
25629585	3	35	gly	glycopeptide	369:380	arg2	glycopeptide					369:380	a mass spectrometry-based automated glycopeptide identification platform	333:404	a mass spectrometry-based automated glycopeptide identification platform (MAGIC) to identify peptide sequences and glycan compositions directly from intact N-linked glycopeptide collision-induced-dissociation spectra	333:548	In this paper, we present a mass spectrometry-based automated glycopeptide identification platform (MAGIC) to identify peptide sequences and glycan compositions directly from intact N-linked glycopeptide collision-induced-dissociation spectra.
25629585	4	36	theme	present	731:737	arg1	proteins					710:717	proteins	710:717	proteins	710:717	The identification of the Y1 (peptideY0 + GlcNAc) ion is critical for the correct analysis of unknown glycoproteins, especially without prior knowledge of the proteins and glycans present in the sample.
25629585	3	37	theme	peptide	426:432	arg1	sequences					434:442	peptide sequences	426:442	peptide sequences	426:442	In this paper, we present a mass spectrometry-based automated glycopeptide identification platform (MAGIC) to identify peptide sequences and glycan compositions directly from intact N-linked glycopeptide collision-induced-dissociation spectra.
25629585	8	38	theme	2-	1390:1391	arg1	increase					1404:1411	a 2- to 31-fold increase	1388:1411	a 2- to 31-fold increase in the relative intensities of the peptide fragments	1388:1464	For the model glycoprotein horseradish peroxidase (HRP) and a 5-glycoprotein mixture, a 2- to 31-fold increase in the relative intensities of the peptide fragments was achieved, which led to the identification of 7 tryptic glycopeptides from HRP and 16 glycopeptides from the mixture via Mascot.
25629585	0	39	theme	N-linked	20:27	arg1	tool					57:60	an automated N-linked glycoprotein identification tool	7:60	MAGIC: an automated N-linked glycoprotein identification tool	0:60	MAGIC: an automated N-linked glycoprotein identification tool using a Y1-ion pattern matching algorithm and in silico MS² approach.
25629585	6	40	theme	sequence	1035:1042	arg1	identification					1044:1057	the subsequent peptide sequence identification	1012:1057	the subsequent peptide sequence identification by common database search engines	1012:1091	To facilitate the subsequent peptide sequence identification by common database search engines, MAGIC generates in silico spectra by overwriting the original precursor with the naked peptide m/z and removing all of the glycan-related ions.
25629585	10	41	theme	false	1772:1776	arg1	rate					1788:1791	a remarkable false discovery rate	1759:1791	a remarkable false discovery rate of 0	1759:1796	Finally, a remarkable false discovery rate of 0 was achieved on the N-glycosylation-free Escherichia coli data set.
25629585	5	42	dep	Y1	889:890	arg1	Y1					913:914	Y1	913:914	Y1	913:914	To ensure accurate Y1-ion assignment, we propose a novel algorithm called Trident that detects a triplet pattern corresponding to [Y0, Y1, Y2] or [Y0-NH3, Y0, Y1] from the fragmentation of the common trimannosyl core of N-linked glycopeptides.
25629585	0	43	theme	identification	42:55	arg1	tool					57:60	an automated N-linked glycoprotein identification tool	7:60	MAGIC: an automated N-linked glycoprotein identification tool	0:60	MAGIC: an automated N-linked glycoprotein identification tool using a Y1-ion pattern matching algorithm and in silico MS² approach.
25629585	10	44	dep	Escherichia	1839:1849	arg1	coli					1851:1854	coli	1851:1854	coli	1851:1854	Finally, a remarkable false discovery rate of 0 was achieved on the N-glycosylation-free Escherichia coli data set.
25629585	3	45	theme	collision-induced-dissociation	511:540	arg1	spectra					542:548	intact N-linked glycopeptide collision-induced-dissociation spectra	482:548	intact N-linked glycopeptide collision-induced-dissociation spectra	482:548	In this paper, we present a mass spectrometry-based automated glycopeptide identification platform (MAGIC) to identify peptide sequences and glycan compositions directly from intact N-linked glycopeptide collision-induced-dissociation spectra.
25629585	9	46	gly	glycopeptides	1713:1725	arg2	glycopeptides					1713:1725	36 glycopeptides	1710:1725	36 glycopeptides from 26 glycoproteins	1710:1747	In the HeLa cell proteome data set, MAGIC processed over a thousand MS(2) spectra in 3 min on a PC and reported 36 glycopeptides from 26 glycoproteins.
25629585	9	46	gly	glycopeptides	1713:1725	arg1	glycoproteins					1735:1747	26 glycoproteins	1732:1747	26 glycoproteins	1732:1747	In the HeLa cell proteome data set, MAGIC processed over a thousand MS(2) spectra in 3 min on a PC and reported 36 glycopeptides from 26 glycoproteins.
25629585	1	47	theme	proteins	223:230	arg1	activities					209:218	activities	209:218	activities	209:218	Glycosylation is a highly complex modification influencing the functions and activities of proteins.
25629585	1	47	theme	proteins	223:230	arg1	functions					195:203	functions	195:203	functions	195:203	Glycosylation is a highly complex modification influencing the functions and activities of proteins.
25629585	9	48	from	min	1685:1687	arg1	PC					1694:1695	a PC	1692:1695	a PC	1692:1695	In the HeLa cell proteome data set, MAGIC processed over a thousand MS(2) spectra in 3 min on a PC and reported 36 glycopeptides from 26 glycoproteins.
25629585	5	49	theme	glycopeptides	983:995	arg1	core					966:969	the common trimannosyl core	943:969	the common trimannosyl core of N-linked glycopeptides	943:995	To ensure accurate Y1-ion assignment, we propose a novel algorithm called Trident that detects a triplet pattern corresponding to [Y0, Y1, Y2] or [Y0-NH3, Y0, Y1] from the fragmentation of the common trimannosyl core of N-linked glycopeptides.
25629585	8	50	theme	model	1310:1314	arg1	HRP					1353:1355	HRP	1353:1355	HRP	1353:1355	For the model glycoprotein horseradish peroxidase (HRP) and a 5-glycoprotein mixture, a 2- to 31-fold increase in the relative intensities of the peptide fragments was achieved, which led to the identification of 7 tryptic glycopeptides from HRP and 16 glycopeptides from the mixture via Mascot.
25629585	8	50	theme	model	1310:1314	arg1	peroxidase					1341:1350	the model glycoprotein horseradish peroxidase	1306:1350	the model glycoprotein horseradish peroxidase (HRP)	1306:1356	For the model glycoprotein horseradish peroxidase (HRP) and a 5-glycoprotein mixture, a 2- to 31-fold increase in the relative intensities of the peptide fragments was achieved, which led to the identification of 7 tryptic glycopeptides from HRP and 16 glycopeptides from the mixture via Mascot.
25629585	10	51	theme	0	1796:1796	arg1	rate					1788:1791	a remarkable false discovery rate	1759:1791	a remarkable false discovery rate of 0	1759:1796	Finally, a remarkable false discovery rate of 0 was achieved on the N-glycosylation-free Escherichia coli data set.
25629585	1	52	dep	functions	195:203	arg1	the					191:193	the	191:193	the	191:193	Glycosylation is a highly complex modification influencing the functions and activities of proteins.
25629585	5	53	from	fragmentation	926:938	arg1	[Y0					884:886	[Y0	884:886	[Y0	884:886	To ensure accurate Y1-ion assignment, we propose a novel algorithm called Trident that detects a triplet pattern corresponding to [Y0, Y1, Y2] or [Y0-NH3, Y0, Y1] from the fragmentation of the common trimannosyl core of N-linked glycopeptides.
25629585	5	53	from	fragmentation	926:938	arg1	[Y0-NH3					900:906	[Y0-NH3	900:906	[Y0-NH3	900:906	To ensure accurate Y1-ion assignment, we propose a novel algorithm called Trident that detects a triplet pattern corresponding to [Y0, Y1, Y2] or [Y0-NH3, Y0, Y1] from the fragmentation of the common trimannosyl core of N-linked glycopeptides.
25629585	8	54	gly	glycopeptides	1525:1537	arg1	HRP					1544:1546	HRP	1544:1546	HRP	1544:1546	For the model glycoprotein horseradish peroxidase (HRP) and a 5-glycoprotein mixture, a 2- to 31-fold increase in the relative intensities of the peptide fragments was achieved, which led to the identification of 7 tryptic glycopeptides from HRP and 16 glycopeptides from the mixture via Mascot.
25629585	8	54	gly	glycopeptides	1525:1537	arg2	glycopeptides					1525:1537	7 tryptic glycopeptides	1515:1537	7 tryptic glycopeptides from HRP	1515:1546	For the model glycoprotein horseradish peroxidase (HRP) and a 5-glycoprotein mixture, a 2- to 31-fold increase in the relative intensities of the peptide fragments was achieved, which led to the identification of 7 tryptic glycopeptides from HRP and 16 glycopeptides from the mixture via Mascot.
25629585	8	54	gly	glycopeptides	1525:1537	arg1	mixture					1578:1584	the mixture	1574:1584	the mixture via Mascot	1574:1595	For the model glycoprotein horseradish peroxidase (HRP) and a 5-glycoprotein mixture, a 2- to 31-fold increase in the relative intensities of the peptide fragments was achieved, which led to the identification of 7 tryptic glycopeptides from HRP and 16 glycopeptides from the mixture via Mascot.
25629585	0	55	dep	MAGIC	0:4	arg1	tool					57:60	an automated N-linked glycoprotein identification tool	7:60	MAGIC: an automated N-linked glycoprotein identification tool	0:60	MAGIC: an automated N-linked glycoprotein identification tool using a Y1-ion pattern matching algorithm and in silico MS² approach.
25629585	6	56	theme	in	1110:1111	arg1	spectra					1120:1126	in silico spectra	1110:1126	in silico spectra	1110:1126	To facilitate the subsequent peptide sequence identification by common database search engines, MAGIC generates in silico spectra by overwriting the original precursor with the naked peptide m/z and removing all of the glycan-related ions.
25629585	4	57	theme	glycoproteins	653:665	arg1	analysis					633:640	the correct analysis	621:640	the correct analysis of unknown glycoproteins	621:665	The identification of the Y1 (peptideY0 + GlcNAc) ion is critical for the correct analysis of unknown glycoproteins, especially without prior knowledge of the proteins and glycans present in the sample.
25629585	10	58	theme	Escherichia	1839:1849	arg1	set					1861:1863	the N-glycosylation-free Escherichia coli data set	1814:1863	the N-glycosylation-free Escherichia coli data set	1814:1863	Finally, a remarkable false discovery rate of 0 was achieved on the N-glycosylation-free Escherichia coli data set.
25629585	10	59	theme	data	1856:1859	arg1	set					1861:1863	the N-glycosylation-free Escherichia coli data set	1814:1863	the N-glycosylation-free Escherichia coli data set	1814:1863	Finally, a remarkable false discovery rate of 0 was achieved on the N-glycosylation-free Escherichia coli data set.
25629585	9	60	theme	data	1624:1627	arg1	set					1629:1631	the HeLa cell proteome data set	1601:1631	the HeLa cell proteome data set	1601:1631	In the HeLa cell proteome data set, MAGIC processed over a thousand MS(2) spectra in 3 min on a PC and reported 36 glycopeptides from 26 glycoproteins.
25629585	9	61	from	glycoproteins	1735:1747	arg1	glycopeptides					1713:1725	36 glycopeptides	1710:1725	36 glycopeptides from 26 glycoproteins	1710:1747	In the HeLa cell proteome data set, MAGIC processed over a thousand MS(2) spectra in 3 min on a PC and reported 36 glycopeptides from 26 glycoproteins.
25629585	4	62	theme	correct	625:631	arg1	analysis					633:640	the correct analysis	621:640	the correct analysis of unknown glycoproteins	621:665	The identification of the Y1 (peptideY0 + GlcNAc) ion is critical for the correct analysis of unknown glycoproteins, especially without prior knowledge of the proteins and glycans present in the sample.
25629585	4	63	attach	present	731:737	arg2	proteins					710:717	proteins	710:717	proteins	710:717	The identification of the Y1 (peptideY0 + GlcNAc) ion is critical for the correct analysis of unknown glycoproteins, especially without prior knowledge of the proteins and glycans present in the sample.
25629585	4	63	attach	present	731:737	arg1	sample					746:751	the sample	742:751	the sample	742:751	The identification of the Y1 (peptideY0 + GlcNAc) ion is critical for the correct analysis of unknown glycoproteins, especially without prior knowledge of the proteins and glycans present in the sample.
25629585	1	64	theme	complex	158:164	arg1	modification					166:177	a highly complex modification	149:177	a highly complex modification influencing the functions and activities of proteins	149:230	Glycosylation is a highly complex modification influencing the functions and activities of proteins.
25629585	1	64	theme	complex	158:164	arg1	Glycosylation					132:144	Glycosylation	132:144	Glycosylation	132:144	Glycosylation is a highly complex modification influencing the functions and activities of proteins.
25629585	8	65	from	identification	1497:1510	arg1	HRP					1544:1546	HRP	1544:1546	HRP	1544:1546	For the model glycoprotein horseradish peroxidase (HRP) and a 5-glycoprotein mixture, a 2- to 31-fold increase in the relative intensities of the peptide fragments was achieved, which led to the identification of 7 tryptic glycopeptides from HRP and 16 glycopeptides from the mixture via Mascot.
25629585	8	65	from	identification	1497:1510	arg1	mixture					1578:1584	the mixture	1574:1584	the mixture via Mascot	1574:1595	For the model glycoprotein horseradish peroxidase (HRP) and a 5-glycoprotein mixture, a 2- to 31-fold increase in the relative intensities of the peptide fragments was achieved, which led to the identification of 7 tryptic glycopeptides from HRP and 16 glycopeptides from the mixture via Mascot.
25629585	6	66	theme	naked	1175:1179	arg1	m/z					1189:1191	the naked peptide m/z	1171:1191	the naked peptide m/z	1171:1191	To facilitate the subsequent peptide sequence identification by common database search engines, MAGIC generates in silico spectra by overwriting the original precursor with the naked peptide m/z and removing all of the glycan-related ions.
25629585	5	67	theme	novel	805:809	arg1	algorithm					811:819	a novel algorithm	803:819	a novel algorithm called Trident that detects a triplet pattern corresponding to [Y0, Y1, Y2] or [Y0-NH3, Y0, Y1] from the fragmentation of the common trimannosyl core of N-linked glycopeptides	803:995	To ensure accurate Y1-ion assignment, we propose a novel algorithm called Trident that detects a triplet pattern corresponding to [Y0, Y1, Y2] or [Y0-NH3, Y0, Y1] from the fragmentation of the common trimannosyl core of N-linked glycopeptides.
25629585	3	68	from	spectra	542:548	arg1	compositions					455:466	glycan compositions	448:466	glycan compositions	448:466	In this paper, we present a mass spectrometry-based automated glycopeptide identification platform (MAGIC) to identify peptide sequences and glycan compositions directly from intact N-linked glycopeptide collision-induced-dissociation spectra.
25629585	3	68	from	spectra	542:548	arg1	sequences					434:442	peptide sequences	426:442	peptide sequences	426:442	In this paper, we present a mass spectrometry-based automated glycopeptide identification platform (MAGIC) to identify peptide sequences and glycan compositions directly from intact N-linked glycopeptide collision-induced-dissociation spectra.
25629585	4	69	theme	proteins	710:717	arg1	knowledge					693:701	prior knowledge	687:701	prior knowledge of the proteins and glycans present in the sample	687:751	The identification of the Y1 (peptideY0 + GlcNAc) ion is critical for the correct analysis of unknown glycoproteins, especially without prior knowledge of the proteins and glycans present in the sample.
25629585	0	70	theme	MS²	118:120	arg1	approach					122:129	in silico MS² approach	108:129	in silico MS² approach	108:129	MAGIC: an automated N-linked glycoprotein identification tool using a Y1-ion pattern matching algorithm and in silico MS² approach.
25629585	9	71	theme	MS	1666:1667	arg1	spectra					1672:1678	a thousand MS(2) spectra	1655:1678	a thousand MS(2) spectra	1655:1678	In the HeLa cell proteome data set, MAGIC processed over a thousand MS(2) spectra in 3 min on a PC and reported 36 glycopeptides from 26 glycoproteins.
25629585	6	72	theme	original	1147:1154	arg1	precursor					1156:1164	the original precursor	1143:1164	the original precursor with the naked peptide m/z	1143:1191	To facilitate the subsequent peptide sequence identification by common database search engines, MAGIC generates in silico spectra by overwriting the original precursor with the naked peptide m/z and removing all of the glycan-related ions.
25629585	8	73	from	increase	1404:1411	arg1	intensities					1429:1439	the relative intensities	1416:1439	the relative intensities of the peptide fragments	1416:1464	For the model glycoprotein horseradish peroxidase (HRP) and a 5-glycoprotein mixture, a 2- to 31-fold increase in the relative intensities of the peptide fragments was achieved, which led to the identification of 7 tryptic glycopeptides from HRP and 16 glycopeptides from the mixture via Mascot.
25629585	6	74	with	precursor	1156:1164	arg1	m/z					1189:1191	the naked peptide m/z	1171:1191	the naked peptide m/z	1171:1191	To facilitate the subsequent peptide sequence identification by common database search engines, MAGIC generates in silico spectra by overwriting the original precursor with the naked peptide m/z and removing all of the glycan-related ions.
25629585	8	75	theme	peptide	1448:1454	arg1	fragments					1456:1464	the peptide fragments	1444:1464	the peptide fragments	1444:1464	For the model glycoprotein horseradish peroxidase (HRP) and a 5-glycoprotein mixture, a 2- to 31-fold increase in the relative intensities of the peptide fragments was achieved, which led to the identification of 7 tryptic glycopeptides from HRP and 16 glycopeptides from the mixture via Mascot.
25629585	4	76	theme	prior	687:691	arg1	knowledge					693:701	prior knowledge	687:701	prior knowledge of the proteins and glycans present in the sample	687:751	The identification of the Y1 (peptideY0 + GlcNAc) ion is critical for the correct analysis of unknown glycoproteins, especially without prior knowledge of the proteins and glycans present in the sample.
25629585	6	77	theme	glycan-related	1217:1230	arg1	ions					1232:1235	the glycan-related ions	1213:1235	the glycan-related ions	1213:1235	To facilitate the subsequent peptide sequence identification by common database search engines, MAGIC generates in silico spectra by overwriting the original precursor with the naked peptide m/z and removing all of the glycan-related ions.
25629585	10	78	theme	remarkable	1761:1770	arg1	rate					1788:1791	a remarkable false discovery rate	1759:1791	a remarkable false discovery rate of 0	1759:1796	Finally, a remarkable false discovery rate of 0 was achieved on the N-glycosylation-free Escherichia coli data set.
25629585	6	79	theme	peptide	1027:1033	arg1	identification					1044:1057	the subsequent peptide sequence identification	1012:1057	the subsequent peptide sequence identification by common database search engines	1012:1091	To facilitate the subsequent peptide sequence identification by common database search engines, MAGIC generates in silico spectra by overwriting the original precursor with the naked peptide m/z and removing all of the glycan-related ions.
25629585	5	80	theme	common	947:952	arg1	core					966:969	the common trimannosyl core	943:969	the common trimannosyl core of N-linked glycopeptides	943:995	To ensure accurate Y1-ion assignment, we propose a novel algorithm called Trident that detects a triplet pattern corresponding to [Y0, Y1, Y2] or [Y0-NH3, Y0, Y1] from the fragmentation of the common trimannosyl core of N-linked glycopeptides.
25629585	2	81	theme	glycopeptide	258:269	arg1	spectra					271:277	intact glycopeptide spectra	251:277	intact glycopeptide spectra	251:277	Interpretation of intact glycopeptide spectra is crucial but challenging.
25629585	4	82	theme	glycans	723:729	arg1	knowledge					693:701	prior knowledge	687:701	prior knowledge of the proteins and glycans present in the sample	687:751	The identification of the Y1 (peptideY0 + GlcNAc) ion is critical for the correct analysis of unknown glycoproteins, especially without prior knowledge of the proteins and glycans present in the sample.
25629585	8	83	dep	2-	1390:1391	arg1	to					1393:1394	to	1393:1394	to	1393:1394	For the model glycoprotein horseradish peroxidase (HRP) and a 5-glycoprotein mixture, a 2- to 31-fold increase in the relative intensities of the peptide fragments was achieved, which led to the identification of 7 tryptic glycopeptides from HRP and 16 glycopeptides from the mixture via Mascot.
25629585	4	84	theme	Y1	577:578	arg1	ion					601:603	the Y1 (peptideY0 + GlcNAc) ion	573:603	the Y1 (peptideY0 + GlcNAc) ion	573:603	The identification of the Y1 (peptideY0 + GlcNAc) ion is critical for the correct analysis of unknown glycoproteins, especially without prior knowledge of the proteins and glycans present in the sample.
25629585	8	85	theme	horseradish	1329:1339	arg1	HRP					1353:1355	HRP	1353:1355	HRP	1353:1355	For the model glycoprotein horseradish peroxidase (HRP) and a 5-glycoprotein mixture, a 2- to 31-fold increase in the relative intensities of the peptide fragments was achieved, which led to the identification of 7 tryptic glycopeptides from HRP and 16 glycopeptides from the mixture via Mascot.
25629585	8	85	theme	horseradish	1329:1339	arg1	peroxidase					1341:1350	the model glycoprotein horseradish peroxidase	1306:1350	the model glycoprotein horseradish peroxidase (HRP)	1306:1356	For the model glycoprotein horseradish peroxidase (HRP) and a 5-glycoprotein mixture, a 2- to 31-fold increase in the relative intensities of the peptide fragments was achieved, which led to the identification of 7 tryptic glycopeptides from HRP and 16 glycopeptides from the mixture via Mascot.
25629585	5	86	theme	accurate	764:771	arg1	assignment					780:789	accurate Y1-ion assignment	764:789	accurate Y1-ion assignment	764:789	To ensure accurate Y1-ion assignment, we propose a novel algorithm called Trident that detects a triplet pattern corresponding to [Y0, Y1, Y2] or [Y0-NH3, Y0, Y1] from the fragmentation of the common trimannosyl core of N-linked glycopeptides.
25629585	0	87	gly	glycoprotein	29:40	arg1	glycoprotein					29:40	an automated N-linked glycoprotein identification tool	7:60	MAGIC: an automated N-linked glycoprotein identification tool	0:60	MAGIC: an automated N-linked glycoprotein identification tool using a Y1-ion pattern matching algorithm and in silico MS² approach.
25629585	0	88	dep	in	108:109	arg1	silico					111:116	silico	111:116	silico	111:116	MAGIC: an automated N-linked glycoprotein identification tool using a Y1-ion pattern matching algorithm and in silico MS² approach.
25629585	3	89	theme	identification	382:395	arg1	platform					397:404	a mass spectrometry-based automated glycopeptide identification platform	333:404	a mass spectrometry-based automated glycopeptide identification platform (MAGIC) to identify peptide sequences and glycan compositions directly from intact N-linked glycopeptide collision-induced-dissociation spectra	333:548	In this paper, we present a mass spectrometry-based automated glycopeptide identification platform (MAGIC) to identify peptide sequences and glycan compositions directly from intact N-linked glycopeptide collision-induced-dissociation spectra.
25629585	5	90	theme	trimannosyl	954:964	arg1	core					966:969	the common trimannosyl core	943:969	the common trimannosyl core of N-linked glycopeptides	943:995	To ensure accurate Y1-ion assignment, we propose a novel algorithm called Trident that detects a triplet pattern corresponding to [Y0, Y1, Y2] or [Y0-NH3, Y0, Y1] from the fragmentation of the common trimannosyl core of N-linked glycopeptides.
25629585	0	91	theme	in	108:109	arg1	approach					122:129	in silico MS² approach	108:129	in silico MS² approach	108:129	MAGIC: an automated N-linked glycoprotein identification tool using a Y1-ion pattern matching algorithm and in silico MS² approach.
25629585	8	92	gly	glycoprotein	1316:1327	arg1	glycoprotein					1316:1327	the model glycoprotein horseradish peroxidase	1306:1350	the model glycoprotein horseradish peroxidase (HRP)	1306:1356	For the model glycoprotein horseradish peroxidase (HRP) and a 5-glycoprotein mixture, a 2- to 31-fold increase in the relative intensities of the peptide fragments was achieved, which led to the identification of 7 tryptic glycopeptides from HRP and 16 glycopeptides from the mixture via Mascot.
25629585	9	93	gly	glycoproteins	1735:1747	arg1	glycoproteins					1735:1747	26 glycoproteins	1732:1747	26 glycoproteins	1732:1747	In the HeLa cell proteome data set, MAGIC processed over a thousand MS(2) spectra in 3 min on a PC and reported 36 glycopeptides from 26 glycoproteins.
25629585	5	94	theme	core	966:969	arg1	fragmentation					926:938	the fragmentation	922:938	the fragmentation of the common trimannosyl core of N-linked glycopeptides	922:995	To ensure accurate Y1-ion assignment, we propose a novel algorithm called Trident that detects a triplet pattern corresponding to [Y0, Y1, Y2] or [Y0-NH3, Y0, Y1] from the fragmentation of the common trimannosyl core of N-linked glycopeptides.
25629585	3	95	theme	automated	359:367	arg1	platform					397:404	a mass spectrometry-based automated glycopeptide identification platform	333:404	a mass spectrometry-based automated glycopeptide identification platform (MAGIC) to identify peptide sequences and glycan compositions directly from intact N-linked glycopeptide collision-induced-dissociation spectra	333:548	In this paper, we present a mass spectrometry-based automated glycopeptide identification platform (MAGIC) to identify peptide sequences and glycan compositions directly from intact N-linked glycopeptide collision-induced-dissociation spectra.
25629585	3	96	attach	present	325:331	arg2	we					322:323	we	322:323	we	322:323	In this paper, we present a mass spectrometry-based automated glycopeptide identification platform (MAGIC) to identify peptide sequences and glycan compositions directly from intact N-linked glycopeptide collision-induced-dissociation spectra.
25629585	3	96	attach	present	325:331	arg1	paper					315:319	this paper	310:319	this paper	310:319	In this paper, we present a mass spectrometry-based automated glycopeptide identification platform (MAGIC) to identify peptide sequences and glycan compositions directly from intact N-linked glycopeptide collision-induced-dissociation spectra.
25629585	6	97	theme	search	1078:1083	arg1	engines					1085:1091	common database search engines	1062:1091	common database search engines	1062:1091	To facilitate the subsequent peptide sequence identification by common database search engines, MAGIC generates in silico spectra by overwriting the original precursor with the naked peptide m/z and removing all of the glycan-related ions.
25629585	4	98	theme	ion	601:603	arg1	identification					555:568	The identification	551:568	The identification of the Y1 (peptideY0 + GlcNAc) ion	551:603	The identification of the Y1 (peptideY0 + GlcNAc) ion is critical for the correct analysis of unknown glycoproteins, especially without prior knowledge of the proteins and glycans present in the sample.
25629585	4	98	theme	ion	601:603	arg1	critical					608:615	critical	608:615	critical	608:615	The identification of the Y1 (peptideY0 + GlcNAc) ion is critical for the correct analysis of unknown glycoproteins, especially without prior knowledge of the proteins and glycans present in the sample.
25629585	3	99	theme	glycan	448:453	arg1	compositions					455:466	glycan compositions	448:466	glycan compositions	448:466	In this paper, we present a mass spectrometry-based automated glycopeptide identification platform (MAGIC) to identify peptide sequences and glycan compositions directly from intact N-linked glycopeptide collision-induced-dissociation spectra.
25629585	0	100	theme	automated	10:18	arg1	tool					57:60	an automated N-linked glycoprotein identification tool	7:60	MAGIC: an automated N-linked glycoprotein identification tool	0:60	MAGIC: an automated N-linked glycoprotein identification tool using a Y1-ion pattern matching algorithm and in silico MS² approach.
25629585	8	101	theme	relative	1420:1427	arg1	intensities					1429:1439	the relative intensities	1416:1439	the relative intensities of the peptide fragments	1416:1464	For the model glycoprotein horseradish peroxidase (HRP) and a 5-glycoprotein mixture, a 2- to 31-fold increase in the relative intensities of the peptide fragments was achieved, which led to the identification of 7 tryptic glycopeptides from HRP and 16 glycopeptides from the mixture via Mascot.
25629585	6	102	theme	common	1062:1067	arg1	engines					1085:1091	common database search engines	1062:1091	common database search engines	1062:1091	To facilitate the subsequent peptide sequence identification by common database search engines, MAGIC generates in silico spectra by overwriting the original precursor with the naked peptide m/z and removing all of the glycan-related ions.
25629585	3	103	theme	glycopeptide	498:509	arg1	spectra					542:548	intact N-linked glycopeptide collision-induced-dissociation spectra	482:548	intact N-linked glycopeptide collision-induced-dissociation spectra	482:548	In this paper, we present a mass spectrometry-based automated glycopeptide identification platform (MAGIC) to identify peptide sequences and glycan compositions directly from intact N-linked glycopeptide collision-induced-dissociation spectra.
25629585	0	104	theme	glycoprotein	29:40	arg1	tool					57:60	an automated N-linked glycoprotein identification tool	7:60	MAGIC: an automated N-linked glycoprotein identification tool	0:60	MAGIC: an automated N-linked glycoprotein identification tool using a Y1-ion pattern matching algorithm and in silico MS² approach.
25629585	4	105	from	sample	746:751	arg1	present					731:737	present	731:737	present	731:737	The identification of the Y1 (peptideY0 + GlcNAc) ion is critical for the correct analysis of unknown glycoproteins, especially without prior knowledge of the proteins and glycans present in the sample.
25629585	8	106	theme	glycopeptides	1555:1567	arg1	identification					1497:1510	the identification	1493:1510	the identification of 7 tryptic glycopeptides from HRP and 16 glycopeptides from the mixture via Mascot	1493:1595	For the model glycoprotein horseradish peroxidase (HRP) and a 5-glycoprotein mixture, a 2- to 31-fold increase in the relative intensities of the peptide fragments was achieved, which led to the identification of 7 tryptic glycopeptides from HRP and 16 glycopeptides from the mixture via Mascot.
25629585	10	107	theme	discovery	1778:1786	arg1	rate					1788:1791	a remarkable false discovery rate	1759:1791	a remarkable false discovery rate of 0	1759:1796	Finally, a remarkable false discovery rate of 0 was achieved on the N-glycosylation-free Escherichia coli data set.
25629585	3	108	theme	intact	482:487	arg1	spectra					542:548	intact N-linked glycopeptide collision-induced-dissociation spectra	482:548	intact N-linked glycopeptide collision-induced-dissociation spectra	482:548	In this paper, we present a mass spectrometry-based automated glycopeptide identification platform (MAGIC) to identify peptide sequences and glycan compositions directly from intact N-linked glycopeptide collision-induced-dissociation spectra.
25629585	8	109	gly	5-glycoprotein	1364:1377	arg1	5-glycoprotein					1364:1377	5-glycoprotein	1364:1377	5-glycoprotein	1364:1377	For the model glycoprotein horseradish peroxidase (HRP) and a 5-glycoprotein mixture, a 2- to 31-fold increase in the relative intensities of the peptide fragments was achieved, which led to the identification of 7 tryptic glycopeptides from HRP and 16 glycopeptides from the mixture via Mascot.
25629585	8	110	theme	tryptic	1517:1523	arg1	glycopeptides					1525:1537	7 tryptic glycopeptides	1515:1537	7 tryptic glycopeptides from HRP	1515:1546	For the model glycoprotein horseradish peroxidase (HRP) and a 5-glycoprotein mixture, a 2- to 31-fold increase in the relative intensities of the peptide fragments was achieved, which led to the identification of 7 tryptic glycopeptides from HRP and 16 glycopeptides from the mixture via Mascot.
25629585	6	111	dep	in	1110:1111	arg1	silico					1113:1118	silico	1113:1118	silico	1113:1118	To facilitate the subsequent peptide sequence identification by common database search engines, MAGIC generates in silico spectra by overwriting the original precursor with the naked peptide m/z and removing all of the glycan-related ions.
25629585	9	112	theme	cell	1610:1613	arg1	set					1629:1631	the HeLa cell proteome data set	1601:1631	the HeLa cell proteome data set	1601:1631	In the HeLa cell proteome data set, MAGIC processed over a thousand MS(2) spectra in 3 min on a PC and reported 36 glycopeptides from 26 glycoproteins.
25629585	5	113	theme	N-linked	974:981	arg1	glycopeptides					983:995	N-linked glycopeptides	974:995	N-linked glycopeptides	974:995	To ensure accurate Y1-ion assignment, we propose a novel algorithm called Trident that detects a triplet pattern corresponding to [Y0, Y1, Y2] or [Y0-NH3, Y0, Y1] from the fragmentation of the common trimannosyl core of N-linked glycopeptides.
25629585	7	114	theme	glycan	1266:1271	arg1	compositions					1273:1284	the glycan compositions	1262:1284	the glycan compositions	1262:1284	Finally, MAGIC computes the glycan compositions and ranks them.
25629585	4	115	theme	unknown	645:651	arg1	glycoproteins					653:665	unknown glycoproteins	645:665	unknown glycoproteins	645:665	The identification of the Y1 (peptideY0 + GlcNAc) ion is critical for the correct analysis of unknown glycoproteins, especially without prior knowledge of the proteins and glycans present in the sample.
25629585	9	116	theme	HeLa	1605:1608	arg1	set					1629:1631	the HeLa cell proteome data set	1601:1631	the HeLa cell proteome data set	1601:1631	In the HeLa cell proteome data set, MAGIC processed over a thousand MS(2) spectra in 3 min on a PC and reported 36 glycopeptides from 26 glycoproteins.
27112859	7	0	theme	mechanical	1197:1206	arg1	properties					1208:1217	the mechanical properties	1193:1217	the mechanical properties of chitosan-GO composites showing that higher molecular weight chitosan composite (GOCC-1000) showed best strength (higher than 3GPa) compared to other composite films	1193:1385	Moreover, mechanical analysis confirmed the effect of molecular weight on the mechanical properties of chitosan-GO composites showing that higher molecular weight chitosan composite (GOCC-1000) showed best strength (higher than 3GPa) compared to other composite films.
27112859	5	1	from	groups	943:948	arg1	surface					957:963	the surface	953:963	the surface of GO	953:969	FTIR studies revealed the successful synthesis of GO from graphite powder and it was confirmed that homogenous blending of chitosan and GO was promising due to oxygenated functional groups on the surface of GO.
27112859	8	2	dep	56	1475:1476	arg1	to					1472:1473	to	1472:1473	to	1472:1473	Thermal stability of GOCC-1000 was enhanced for which residual content increased up to 56% as compared to the thermal stability of GOCC-200 whose residue was restricted to only 24%.
27112859	9	3	theme	composites	1604:1613	arg1	sheets					1615:1620	the composites sheets	1600:1620	the composites sheets	1600:1620	The morphological analysis of the composites sheets by SEM was smooth having dense structure and showed excellent interaction, miscibility, compatibility and dispersion of GO with chitosan.
27112859	1	4	theme	standard	171:178	arg1	method					188:193	standard Hummers method	171:193	standard Hummers method	171:193	Graphene oxide (GO) was indigenously synthesized from graphite using standard Hummers method.
27112859	7	5	theme	composite	1371:1379	arg1	films					1381:1385	other composite films	1365:1385	other composite films	1365:1385	Moreover, mechanical analysis confirmed the effect of molecular weight on the mechanical properties of chitosan-GO composites showing that higher molecular weight chitosan composite (GOCC-1000) showed best strength (higher than 3GPa) compared to other composite films.
27112859	7	6	theme	mechanical	1129:1138	arg1	analysis					1140:1147	mechanical analysis	1129:1147	mechanical analysis	1129:1147	Moreover, mechanical analysis confirmed the effect of molecular weight on the mechanical properties of chitosan-GO composites showing that higher molecular weight chitosan composite (GOCC-1000) showed best strength (higher than 3GPa) compared to other composite films.
27112859	2	7	theme	acetic	317:322	arg1	solvent					334:340	a solvent	332:340	a solvent for chitosan	332:353	Chitosan-graphene oxide green composite films were fabricated by mixing aqueous solution of chitosan and GO using dilute acetic acid as a solvent for chitosan.
27112859	2	7	theme	acetic	317:322	arg1	acid					324:327	dilute acetic acid	310:327	dilute acetic acid	310:327	Chitosan-graphene oxide green composite films were fabricated by mixing aqueous solution of chitosan and GO using dilute acetic acid as a solvent for chitosan.
27112859	4	8	dep	transform	609:617	arg1	infrared					619:626	infrared	619:626	transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), thermogravimetric analysis (TGA) and tensile test	609:758	The structural properties, thermal stability and mechanical properties of the composite films were investigated using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), thermogravimetric analysis (TGA) and tensile test.
27112859	5	9	theme	graphite	819:826	arg1	powder					828:833	graphite powder	819:833	graphite powder	819:833	FTIR studies revealed the successful synthesis of GO from graphite powder and it was confirmed that homogenous blending of chitosan and GO was promising due to oxygenated functional groups on the surface of GO.
27112859	8	10	theme	GOCC-1000	1409:1417	arg1	stability					1396:1404	Thermal stability	1388:1404	Thermal stability of GOCC-1000	1388:1417	Thermal stability of GOCC-1000 was enhanced for which residual content increased up to 56% as compared to the thermal stability of GOCC-200 whose residue was restricted to only 24%.
27112859	5	11	from	powder	828:833	arg1	synthesis					798:806	the successful synthesis	783:806	the successful synthesis of GO from graphite powder	783:833	FTIR studies revealed the successful synthesis of GO from graphite powder and it was confirmed that homogenous blending of chitosan and GO was promising due to oxygenated functional groups on the surface of GO.
27112859	7	12	theme	than	1342:1345	arg1	3GPa					1347:1350	higher than 3GPa	1335:1350	higher than 3GPa	1335:1350	Moreover, mechanical analysis confirmed the effect of molecular weight on the mechanical properties of chitosan-GO composites showing that higher molecular weight chitosan composite (GOCC-1000) showed best strength (higher than 3GPa) compared to other composite films.
27112859	7	12	theme	than	1342:1345	arg1	strength					1325:1332	best strength	1320:1332	best strength (higher than 3GPa)	1320:1351	Moreover, mechanical analysis confirmed the effect of molecular weight on the mechanical properties of chitosan-GO composites showing that higher molecular weight chitosan composite (GOCC-1000) showed best strength (higher than 3GPa) compared to other composite films.
27112859	2	13	theme	composite	226:234	arg1	films					236:240	Chitosan-graphene oxide green composite films	196:240	Chitosan-graphene oxide green composite films	196:240	Chitosan-graphene oxide green composite films were fabricated by mixing aqueous solution of chitosan and GO using dilute acetic acid as a solvent for chitosan.
27112859	4	14	theme	thermogravimetric	710:726	arg1	TGA					738:740	TGA	738:740	TGA	738:740	The structural properties, thermal stability and mechanical properties of the composite films were investigated using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), thermogravimetric analysis (TGA) and tensile test.
27112859	4	14	theme	thermogravimetric	710:726	arg1	analysis					728:735	thermogravimetric analysis	710:735	thermogravimetric analysis (TGA)	710:741	The structural properties, thermal stability and mechanical properties of the composite films were investigated using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), thermogravimetric analysis (TGA) and tensile test.
27112859	1	15	theme	Graphene	102:109	arg1	oxide					111:115	Graphene oxide	102:115	Graphene oxide (GO)	102:120	Graphene oxide (GO) was indigenously synthesized from graphite using standard Hummers method.
27112859	2	16	theme	aqueous	268:274	arg1	solution					276:283	aqueous solution	268:283	aqueous solution of chitosan	268:295	Chitosan-graphene oxide green composite films were fabricated by mixing aqueous solution of chitosan and GO using dilute acetic acid as a solvent for chitosan.
27112859	7	17	theme	best	1320:1323	arg1	3GPa					1347:1350	higher than 3GPa	1335:1350	higher than 3GPa	1335:1350	Moreover, mechanical analysis confirmed the effect of molecular weight on the mechanical properties of chitosan-GO composites showing that higher molecular weight chitosan composite (GOCC-1000) showed best strength (higher than 3GPa) compared to other composite films.
27112859	7	17	theme	best	1320:1323	arg1	strength					1325:1332	best strength	1320:1332	best strength (higher than 3GPa)	1320:1351	Moreover, mechanical analysis confirmed the effect of molecular weight on the mechanical properties of chitosan-GO composites showing that higher molecular weight chitosan composite (GOCC-1000) showed best strength (higher than 3GPa) compared to other composite films.
27112859	4	18	theme	electron	683:690	arg1	microscopy					692:701	scanning electron microscopy	674:701	scanning electron microscopy (SEM)	674:707	The structural properties, thermal stability and mechanical properties of the composite films were investigated using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), thermogravimetric analysis (TGA) and tensile test.
27112859	4	18	theme	electron	683:690	arg1	SEM					704:706	SEM	704:706	SEM	704:706	The structural properties, thermal stability and mechanical properties of the composite films were investigated using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), thermogravimetric analysis (TGA) and tensile test.
27112859	0	19	theme	synthesized	61:71	arg1	oxide					82:86	indigenously synthesized graphene oxide	48:86	indigenously synthesized graphene oxide	48:86	Novel green nano composites films fabricated by indigenously synthesized graphene oxide and chitosan.
27112859	0	20	theme	nano	12:15	arg1	films					28:32	Novel green nano composites films	0:32	Novel green nano composites films	0:32	Novel green nano composites films fabricated by indigenously synthesized graphene oxide and chitosan.
27112859	7	21	theme	weight	1183:1188	arg1	effect					1163:1168	the effect	1159:1168	the effect of molecular weight on the mechanical properties of chitosan-GO composites showing that higher molecular weight chitosan composite (GOCC-1000) showed best strength (higher than 3GPa) compared to other composite films	1159:1385	Moreover, mechanical analysis confirmed the effect of molecular weight on the mechanical properties of chitosan-GO composites showing that higher molecular weight chitosan composite (GOCC-1000) showed best strength (higher than 3GPa) compared to other composite films.
27112859	5	22	theme	chitosan	884:891	arg1	blending					872:879	homogenous blending	861:879	homogenous blending of chitosan	861:891	FTIR studies revealed the successful synthesis of GO from graphite powder and it was confirmed that homogenous blending of chitosan and GO was promising due to oxygenated functional groups on the surface of GO.
27112859	4	23	theme	thermal	510:516	arg1	stability					518:526	thermal stability	510:526	thermal stability	510:526	The structural properties, thermal stability and mechanical properties of the composite films were investigated using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), thermogravimetric analysis (TGA) and tensile test.
27112859	4	24	theme	tensile	747:753	arg1	test					755:758	tensile test	747:758	tensile test	747:758	The structural properties, thermal stability and mechanical properties of the composite films were investigated using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), thermogravimetric analysis (TGA) and tensile test.
27112859	9	25	theme	GO	1742:1743	arg1	compatibility					1710:1722	compatibility	1710:1722	compatibility	1710:1722	The morphological analysis of the composites sheets by SEM was smooth having dense structure and showed excellent interaction, miscibility, compatibility and dispersion of GO with chitosan.
27112859	9	25	theme	GO	1742:1743	arg1	miscibility					1697:1707	miscibility	1697:1707	miscibility	1697:1707	The morphological analysis of the composites sheets by SEM was smooth having dense structure and showed excellent interaction, miscibility, compatibility and dispersion of GO with chitosan.
27112859	9	25	theme	GO	1742:1743	arg1	interaction					1684:1694	excellent interaction	1674:1694	excellent interaction	1674:1694	The morphological analysis of the composites sheets by SEM was smooth having dense structure and showed excellent interaction, miscibility, compatibility and dispersion of GO with chitosan.
27112859	9	25	theme	GO	1742:1743	arg1	dispersion					1728:1737	dispersion	1728:1737	dispersion	1728:1737	The morphological analysis of the composites sheets by SEM was smooth having dense structure and showed excellent interaction, miscibility, compatibility and dispersion of GO with chitosan.
27112859	6	26	located	observed	1044:1051	arg2	peak					1039:1042	its strong peak	1028:1042	its strong peak observed at 11.06°	1028:1061	XRD indicated effective conversion of graphite to GO as its strong peak observed at 11.06° as compared to pristine graphite which appeared at 26°.
27112859	6	26	located	observed	1044:1051	arg1	11.06°					1056:1061	11.06°	1056:1061	11.06°	1056:1061	XRD indicated effective conversion of graphite to GO as its strong peak observed at 11.06° as compared to pristine graphite which appeared at 26°.
27112859	9	27	with	interaction	1684:1694	arg1	chitosan					1750:1757	chitosan	1750:1757	chitosan	1750:1757	The morphological analysis of the composites sheets by SEM was smooth having dense structure and showed excellent interaction, miscibility, compatibility and dispersion of GO with chitosan.
27112859	0	28	theme	Novel	0:4	arg1	films					28:32	Novel green nano composites films	0:32	Novel green nano composites films	0:32	Novel green nano composites films fabricated by indigenously synthesized graphene oxide and chitosan.
27112859	3	29	theme	different	368:376	arg1	viscosity					378:386	different viscosity	368:386	different viscosity	368:386	Chitosan of different viscosity and calculated molecular weight was used keeping amount of GO constant in each composite film.
27112859	8	30	theme	thermal	1498:1504	arg1	stability					1506:1514	the thermal stability	1494:1514	the thermal stability of GOCC-200 whose residue was restricted to only 24%	1494:1567	Thermal stability of GOCC-1000 was enhanced for which residual content increased up to 56% as compared to the thermal stability of GOCC-200 whose residue was restricted to only 24%.
27112859	5	31	theme	GO	811:812	arg1	synthesis					798:806	the successful synthesis	783:806	the successful synthesis of GO from graphite powder	783:833	FTIR studies revealed the successful synthesis of GO from graphite powder and it was confirmed that homogenous blending of chitosan and GO was promising due to oxygenated functional groups on the surface of GO.
27112859	5	32	theme	functional	932:941	arg1	groups					943:948	oxygenated functional groups	921:948	oxygenated functional groups on the surface of GO	921:969	FTIR studies revealed the successful synthesis of GO from graphite powder and it was confirmed that homogenous blending of chitosan and GO was promising due to oxygenated functional groups on the surface of GO.
27112859	7	33	theme	composite	1291:1299	arg1	chitosan					1282:1289	higher molecular weight chitosan composite	1258:1299	higher molecular weight chitosan composite (GOCC-1000)	1258:1311	Moreover, mechanical analysis confirmed the effect of molecular weight on the mechanical properties of chitosan-GO composites showing that higher molecular weight chitosan composite (GOCC-1000) showed best strength (higher than 3GPa) compared to other composite films.
27112859	7	33	theme	composite	1291:1299	arg1	GOCC-1000					1302:1310	GOCC-1000	1302:1310	GOCC-1000	1302:1310	Moreover, mechanical analysis confirmed the effect of molecular weight on the mechanical properties of chitosan-GO composites showing that higher molecular weight chitosan composite (GOCC-1000) showed best strength (higher than 3GPa) compared to other composite films.
27112859	4	34	theme	films	571:575	arg1	properties					543:552	mechanical properties	532:552	mechanical properties	532:552	The structural properties, thermal stability and mechanical properties of the composite films were investigated using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), thermogravimetric analysis (TGA) and tensile test.
27112859	4	34	theme	films	571:575	arg1	properties					498:507	The structural properties	483:507	The structural properties	483:507	The structural properties, thermal stability and mechanical properties of the composite films were investigated using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), thermogravimetric analysis (TGA) and tensile test.
27112859	4	34	theme	films	571:575	arg1	stability					518:526	thermal stability	510:526	thermal stability	510:526	The structural properties, thermal stability and mechanical properties of the composite films were investigated using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), thermogravimetric analysis (TGA) and tensile test.
27112859	3	35	theme	constant	450:457	arg1	amount					437:442	amount	437:442	amount of GO constant	437:457	Chitosan of different viscosity and calculated molecular weight was used keeping amount of GO constant in each composite film.
27112859	3	35	theme	constant	450:457	arg1	constant					450:457	GO constant	447:457	GO constant	447:457	Chitosan of different viscosity and calculated molecular weight was used keeping amount of GO constant in each composite film.
27112859	9	36	dep	smooth	1633:1638	arg1	having					1640:1645	having	1640:1645	having dense structure	1640:1661	The morphological analysis of the composites sheets by SEM was smooth having dense structure and showed excellent interaction, miscibility, compatibility and dispersion of GO with chitosan.
27112859	9	36	dep	smooth	1633:1638	arg1	smooth					1633:1638	smooth	1633:1638	smooth	1633:1638	The morphological analysis of the composites sheets by SEM was smooth having dense structure and showed excellent interaction, miscibility, compatibility and dispersion of GO with chitosan.
27112859	9	36	dep	smooth	1633:1638	arg1	analysis					1588:1595	The morphological analysis	1570:1595	The morphological analysis of the composites sheets by SEM	1570:1627	The morphological analysis of the composites sheets by SEM was smooth having dense structure and showed excellent interaction, miscibility, compatibility and dispersion of GO with chitosan.
27112859	7	37	theme	weight	1275:1280	arg1	chitosan					1282:1289	higher molecular weight chitosan composite	1258:1299	higher molecular weight chitosan composite (GOCC-1000)	1258:1311	Moreover, mechanical analysis confirmed the effect of molecular weight on the mechanical properties of chitosan-GO composites showing that higher molecular weight chitosan composite (GOCC-1000) showed best strength (higher than 3GPa) compared to other composite films.
27112859	7	37	theme	weight	1275:1280	arg1	GOCC-1000					1302:1310	GOCC-1000	1302:1310	GOCC-1000	1302:1310	Moreover, mechanical analysis confirmed the effect of molecular weight on the mechanical properties of chitosan-GO composites showing that higher molecular weight chitosan composite (GOCC-1000) showed best strength (higher than 3GPa) compared to other composite films.
27112859	3	38	theme	molecular	403:411	arg1	weight					413:418	calculated molecular weight	392:418	calculated molecular weight	392:418	Chitosan of different viscosity and calculated molecular weight was used keeping amount of GO constant in each composite film.
27112859	7	39	theme	higher	1258:1263	arg1	chitosan					1282:1289	higher molecular weight chitosan composite	1258:1299	higher molecular weight chitosan composite (GOCC-1000)	1258:1311	Moreover, mechanical analysis confirmed the effect of molecular weight on the mechanical properties of chitosan-GO composites showing that higher molecular weight chitosan composite (GOCC-1000) showed best strength (higher than 3GPa) compared to other composite films.
27112859	7	39	theme	higher	1258:1263	arg1	GOCC-1000					1302:1310	GOCC-1000	1302:1310	GOCC-1000	1302:1310	Moreover, mechanical analysis confirmed the effect of molecular weight on the mechanical properties of chitosan-GO composites showing that higher molecular weight chitosan composite (GOCC-1000) showed best strength (higher than 3GPa) compared to other composite films.
27112859	10	40	theme	composite	1773:1781	arg1	films					1783:1787	The prepared composite films	1760:1787	The prepared composite films	1760:1787	The prepared composite films find their applications as biomaterials in different biomedical fields.
27112859	2	41	theme	oxide	214:218	arg1	films					236:240	Chitosan-graphene oxide green composite films	196:240	Chitosan-graphene oxide green composite films	196:240	Chitosan-graphene oxide green composite films were fabricated by mixing aqueous solution of chitosan and GO using dilute acetic acid as a solvent for chitosan.
27112859	8	42	theme	Thermal	1388:1394	arg1	stability					1396:1404	Thermal stability	1388:1404	Thermal stability of GOCC-1000	1388:1417	Thermal stability of GOCC-1000 was enhanced for which residual content increased up to 56% as compared to the thermal stability of GOCC-200 whose residue was restricted to only 24%.
27112859	5	43	theme	GO	968:969	arg1	surface					957:963	the surface	953:963	the surface of GO	953:969	FTIR studies revealed the successful synthesis of GO from graphite powder and it was confirmed that homogenous blending of chitosan and GO was promising due to oxygenated functional groups on the surface of GO.
27112859	1	44	dep	oxide	111:115	arg1	GO					118:119	GO	118:119	GO	118:119	Graphene oxide (GO) was indigenously synthesized from graphite using standard Hummers method.
27112859	6	45	theme	graphite	1010:1017	arg1	conversion					996:1005	effective conversion	986:1005	effective conversion of graphite to GO as its strong peak observed at 11.06° as compared to pristine graphite which appeared at 26°	986:1116	XRD indicated effective conversion of graphite to GO as its strong peak observed at 11.06° as compared to pristine graphite which appeared at 26°.
27112859	7	46	theme	chitosan-GO	1222:1232	arg1	composites					1234:1243	chitosan-GO composites	1222:1243	chitosan-GO composites showing that higher molecular weight chitosan composite (GOCC-1000) showed best strength (higher than 3GPa) compared to other composite films	1222:1385	Moreover, mechanical analysis confirmed the effect of molecular weight on the mechanical properties of chitosan-GO composites showing that higher molecular weight chitosan composite (GOCC-1000) showed best strength (higher than 3GPa) compared to other composite films.
27112859	4	47	theme	FTIR	629:632	arg1	spectroscopy					635:646	(FTIR) spectroscopy	628:646	(FTIR) spectroscopy	628:646	The structural properties, thermal stability and mechanical properties of the composite films were investigated using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), thermogravimetric analysis (TGA) and tensile test.
27112859	10	48	from	biomaterials	1816:1827	arg1	fields					1853:1858	different biomedical fields	1832:1858	different biomedical fields	1832:1858	The prepared composite films find their applications as biomaterials in different biomedical fields.
27112859	9	49	theme	sheets	1615:1620	arg1	having					1640:1645	having	1640:1645	having dense structure	1640:1661	The morphological analysis of the composites sheets by SEM was smooth having dense structure and showed excellent interaction, miscibility, compatibility and dispersion of GO with chitosan.
27112859	9	49	theme	sheets	1615:1620	arg1	smooth					1633:1638	smooth	1633:1638	smooth	1633:1638	The morphological analysis of the composites sheets by SEM was smooth having dense structure and showed excellent interaction, miscibility, compatibility and dispersion of GO with chitosan.
27112859	9	49	theme	sheets	1615:1620	arg1	analysis					1588:1595	The morphological analysis	1570:1595	The morphological analysis of the composites sheets by SEM	1570:1627	The morphological analysis of the composites sheets by SEM was smooth having dense structure and showed excellent interaction, miscibility, compatibility and dispersion of GO with chitosan.
27112859	2	50	theme	chitosan	288:295	arg1	solution					276:283	aqueous solution	268:283	aqueous solution of chitosan	268:295	Chitosan-graphene oxide green composite films were fabricated by mixing aqueous solution of chitosan and GO using dilute acetic acid as a solvent for chitosan.
27112859	1	51	theme	Hummers	180:186	arg1	method					188:193	standard Hummers method	171:193	standard Hummers method	171:193	Graphene oxide (GO) was indigenously synthesized from graphite using standard Hummers method.
27112859	2	52	theme	dilute	310:315	arg1	solvent					334:340	a solvent	332:340	a solvent for chitosan	332:353	Chitosan-graphene oxide green composite films were fabricated by mixing aqueous solution of chitosan and GO using dilute acetic acid as a solvent for chitosan.
27112859	2	52	theme	dilute	310:315	arg1	acid					324:327	dilute acetic acid	310:327	dilute acetic acid	310:327	Chitosan-graphene oxide green composite films were fabricated by mixing aqueous solution of chitosan and GO using dilute acetic acid as a solvent for chitosan.
27112859	9	53	theme	dense	1647:1651	arg1	structure					1653:1661	dense structure	1647:1661	dense structure	1647:1661	The morphological analysis of the composites sheets by SEM was smooth having dense structure and showed excellent interaction, miscibility, compatibility and dispersion of GO with chitosan.
27112859	7	54	theme	other	1365:1369	arg1	films					1381:1385	other composite films	1365:1385	other composite films	1365:1385	Moreover, mechanical analysis confirmed the effect of molecular weight on the mechanical properties of chitosan-GO composites showing that higher molecular weight chitosan composite (GOCC-1000) showed best strength (higher than 3GPa) compared to other composite films.
27112859	4	55	dep	Fourier	601:607	arg1	transform					609:617	transform	609:617	transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), thermogravimetric analysis (TGA) and tensile test	609:758	The structural properties, thermal stability and mechanical properties of the composite films were investigated using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), thermogravimetric analysis (TGA) and tensile test.
27112859	10	56	theme	prepared	1764:1771	arg1	films					1783:1787	The prepared composite films	1760:1787	The prepared composite films	1760:1787	The prepared composite films find their applications as biomaterials in different biomedical fields.
27112859	7	57	from	effect	1163:1168	arg1	properties					1208:1217	the mechanical properties	1193:1217	the mechanical properties of chitosan-GO composites showing that higher molecular weight chitosan composite (GOCC-1000) showed best strength (higher than 3GPa) compared to other composite films	1193:1385	Moreover, mechanical analysis confirmed the effect of molecular weight on the mechanical properties of chitosan-GO composites showing that higher molecular weight chitosan composite (GOCC-1000) showed best strength (higher than 3GPa) compared to other composite films.
27112859	4	58	theme	X-ray	649:653	arg1	XRD					668:670	XRD	668:670	XRD	668:670	The structural properties, thermal stability and mechanical properties of the composite films were investigated using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), thermogravimetric analysis (TGA) and tensile test.
27112859	4	58	theme	X-ray	649:653	arg1	diffraction					655:665	X-ray diffraction	649:665	X-ray diffraction (XRD)	649:671	The structural properties, thermal stability and mechanical properties of the composite films were investigated using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), thermogravimetric analysis (TGA) and tensile test.
27112859	9	59	with	dispersion	1728:1737	arg1	chitosan					1750:1757	chitosan	1750:1757	chitosan	1750:1757	The morphological analysis of the composites sheets by SEM was smooth having dense structure and showed excellent interaction, miscibility, compatibility and dispersion of GO with chitosan.
27112859	9	60	theme	excellent	1674:1682	arg1	interaction					1684:1694	excellent interaction	1674:1694	excellent interaction	1674:1694	The morphological analysis of the composites sheets by SEM was smooth having dense structure and showed excellent interaction, miscibility, compatibility and dispersion of GO with chitosan.
27112859	8	61	theme	residual	1442:1449	arg1	content					1451:1457	residual content	1442:1457	residual content	1442:1457	Thermal stability of GOCC-1000 was enhanced for which residual content increased up to 56% as compared to the thermal stability of GOCC-200 whose residue was restricted to only 24%.
27112859	9	62	with	compatibility	1710:1722	arg1	chitosan					1750:1757	chitosan	1750:1757	chitosan	1750:1757	The morphological analysis of the composites sheets by SEM was smooth having dense structure and showed excellent interaction, miscibility, compatibility and dispersion of GO with chitosan.
27112859	7	63	theme	higher	1335:1340	arg1	3GPa					1347:1350	higher than 3GPa	1335:1350	higher than 3GPa	1335:1350	Moreover, mechanical analysis confirmed the effect of molecular weight on the mechanical properties of chitosan-GO composites showing that higher molecular weight chitosan composite (GOCC-1000) showed best strength (higher than 3GPa) compared to other composite films.
27112859	7	63	theme	higher	1335:1340	arg1	strength					1325:1332	best strength	1320:1332	best strength (higher than 3GPa)	1320:1351	Moreover, mechanical analysis confirmed the effect of molecular weight on the mechanical properties of chitosan-GO composites showing that higher molecular weight chitosan composite (GOCC-1000) showed best strength (higher than 3GPa) compared to other composite films.
27112859	6	64	theme	pristine	1078:1085	arg1	graphite					1087:1094	pristine graphite	1078:1094	pristine graphite which appeared at 26°	1078:1116	XRD indicated effective conversion of graphite to GO as its strong peak observed at 11.06° as compared to pristine graphite which appeared at 26°.
27112859	0	65	theme	graphene	73:80	arg1	oxide					82:86	indigenously synthesized graphene oxide	48:86	indigenously synthesized graphene oxide	48:86	Novel green nano composites films fabricated by indigenously synthesized graphene oxide and chitosan.
27112859	9	66	with	miscibility	1697:1707	arg1	chitosan					1750:1757	chitosan	1750:1757	chitosan	1750:1757	The morphological analysis of the composites sheets by SEM was smooth having dense structure and showed excellent interaction, miscibility, compatibility and dispersion of GO with chitosan.
27112859	5	67	theme	homogenous	861:870	arg1	blending					872:879	homogenous blending	861:879	homogenous blending of chitosan	861:891	FTIR studies revealed the successful synthesis of GO from graphite powder and it was confirmed that homogenous blending of chitosan and GO was promising due to oxygenated functional groups on the surface of GO.
27112859	6	68	theme	effective	986:994	arg1	conversion					996:1005	effective conversion	986:1005	effective conversion of graphite to GO as its strong peak observed at 11.06° as compared to pristine graphite which appeared at 26°	986:1116	XRD indicated effective conversion of graphite to GO as its strong peak observed at 11.06° as compared to pristine graphite which appeared at 26°.
27112859	4	69	theme	scanning	674:681	arg1	microscopy					692:701	scanning electron microscopy	674:701	scanning electron microscopy (SEM)	674:707	The structural properties, thermal stability and mechanical properties of the composite films were investigated using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), thermogravimetric analysis (TGA) and tensile test.
27112859	4	69	theme	scanning	674:681	arg1	SEM					704:706	SEM	704:706	SEM	704:706	The structural properties, thermal stability and mechanical properties of the composite films were investigated using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), thermogravimetric analysis (TGA) and tensile test.
27112859	7	70	theme	molecular	1173:1181	arg1	weight					1183:1188	molecular weight	1173:1188	molecular weight	1173:1188	Moreover, mechanical analysis confirmed the effect of molecular weight on the mechanical properties of chitosan-GO composites showing that higher molecular weight chitosan composite (GOCC-1000) showed best strength (higher than 3GPa) compared to other composite films.
27112859	10	71	theme	biomedical	1842:1851	arg1	fields					1853:1858	different biomedical fields	1832:1858	different biomedical fields	1832:1858	The prepared composite films find their applications as biomaterials in different biomedical fields.
27112859	4	72	theme	structural	487:496	arg1	properties					498:507	The structural properties	483:507	The structural properties	483:507	The structural properties, thermal stability and mechanical properties of the composite films were investigated using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), thermogravimetric analysis (TGA) and tensile test.
27112859	5	73	theme	successful	787:796	arg1	synthesis					798:806	the successful synthesis	783:806	the successful synthesis of GO from graphite powder	783:833	FTIR studies revealed the successful synthesis of GO from graphite powder and it was confirmed that homogenous blending of chitosan and GO was promising due to oxygenated functional groups on the surface of GO.
27112859	5	74	from	synthesis	798:806	arg1	powder					828:833	graphite powder	819:833	graphite powder	819:833	FTIR studies revealed the successful synthesis of GO from graphite powder and it was confirmed that homogenous blending of chitosan and GO was promising due to oxygenated functional groups on the surface of GO.
27112859	6	75	theme	strong	1032:1037	arg1	peak					1039:1042	its strong peak	1028:1042	its strong peak observed at 11.06°	1028:1061	XRD indicated effective conversion of graphite to GO as its strong peak observed at 11.06° as compared to pristine graphite which appeared at 26°.
27112859	3	76	theme	calculated	392:401	arg1	weight					413:418	calculated molecular weight	392:418	calculated molecular weight	392:418	Chitosan of different viscosity and calculated molecular weight was used keeping amount of GO constant in each composite film.
27112859	4	77	theme	composite	561:569	arg1	films					571:575	the composite films	557:575	the composite films	557:575	The structural properties, thermal stability and mechanical properties of the composite films were investigated using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), thermogravimetric analysis (TGA) and tensile test.
27112859	10	78	theme	different	1832:1840	arg1	fields					1853:1858	different biomedical fields	1832:1858	different biomedical fields	1832:1858	The prepared composite films find their applications as biomaterials in different biomedical fields.
27112859	0	79	theme	composites	17:26	arg1	films					28:32	Novel green nano composites films	0:32	Novel green nano composites films	0:32	Novel green nano composites films fabricated by indigenously synthesized graphene oxide and chitosan.
27112859	5	80	theme	oxygenated	921:930	arg1	groups					943:948	oxygenated functional groups	921:948	oxygenated functional groups on the surface of GO	921:969	FTIR studies revealed the successful synthesis of GO from graphite powder and it was confirmed that homogenous blending of chitosan and GO was promising due to oxygenated functional groups on the surface of GO.
27112859	3	81	theme	viscosity	378:386	arg1	Chitosan					356:363	Chitosan	356:363	Chitosan of different viscosity and calculated molecular weight	356:418	Chitosan of different viscosity and calculated molecular weight was used keeping amount of GO constant in each composite film.
27112859	8	82	theme	GOCC-200	1519:1526	arg1	stability					1506:1514	the thermal stability	1494:1514	the thermal stability of GOCC-200 whose residue was restricted to only 24%	1494:1567	Thermal stability of GOCC-1000 was enhanced for which residual content increased up to 56% as compared to the thermal stability of GOCC-200 whose residue was restricted to only 24%.
27112859	3	83	theme	composite	467:475	arg1	film					477:480	each composite film	462:480	each composite film	462:480	Chitosan of different viscosity and calculated molecular weight was used keeping amount of GO constant in each composite film.
27112859	5	84	theme	FTIR	761:764	arg1	studies					766:772	FTIR studies	761:772	FTIR studies	761:772	FTIR studies revealed the successful synthesis of GO from graphite powder and it was confirmed that homogenous blending of chitosan and GO was promising due to oxygenated functional groups on the surface of GO.
27112859	7	85	theme	molecular	1265:1273	arg1	chitosan					1282:1289	higher molecular weight chitosan composite	1258:1299	higher molecular weight chitosan composite (GOCC-1000)	1258:1311	Moreover, mechanical analysis confirmed the effect of molecular weight on the mechanical properties of chitosan-GO composites showing that higher molecular weight chitosan composite (GOCC-1000) showed best strength (higher than 3GPa) compared to other composite films.
27112859	7	85	theme	molecular	1265:1273	arg1	GOCC-1000					1302:1310	GOCC-1000	1302:1310	GOCC-1000	1302:1310	Moreover, mechanical analysis confirmed the effect of molecular weight on the mechanical properties of chitosan-GO composites showing that higher molecular weight chitosan composite (GOCC-1000) showed best strength (higher than 3GPa) compared to other composite films.
27112859	4	86	theme	mechanical	532:541	arg1	properties					543:552	mechanical properties	532:552	mechanical properties	532:552	The structural properties, thermal stability and mechanical properties of the composite films were investigated using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), thermogravimetric analysis (TGA) and tensile test.
27112859	3	87	theme	weight	413:418	arg1	Chitosan					356:363	Chitosan	356:363	Chitosan of different viscosity and calculated molecular weight	356:418	Chitosan of different viscosity and calculated molecular weight was used keeping amount of GO constant in each composite film.
27112859	2	88	theme	green	220:224	arg1	films					236:240	Chitosan-graphene oxide green composite films	196:240	Chitosan-graphene oxide green composite films	196:240	Chitosan-graphene oxide green composite films were fabricated by mixing aqueous solution of chitosan and GO using dilute acetic acid as a solvent for chitosan.
27112859	3	89	used	used	424:427	arg2	Chitosan					356:363	Chitosan	356:363	Chitosan of different viscosity and calculated molecular weight	356:418	Chitosan of different viscosity and calculated molecular weight was used keeping amount of GO constant in each composite film.
27112859	3	90	dep	constant	450:457	arg1	GO					447:448	GO	447:448	GO	447:448	Chitosan of different viscosity and calculated molecular weight was used keeping amount of GO constant in each composite film.
27112859	7	91	theme	composites	1234:1243	arg1	properties					1208:1217	the mechanical properties	1193:1217	the mechanical properties of chitosan-GO composites showing that higher molecular weight chitosan composite (GOCC-1000) showed best strength (higher than 3GPa) compared to other composite films	1193:1385	Moreover, mechanical analysis confirmed the effect of molecular weight on the mechanical properties of chitosan-GO composites showing that higher molecular weight chitosan composite (GOCC-1000) showed best strength (higher than 3GPa) compared to other composite films.
27112859	2	92	theme	Chitosan-graphene	196:212	arg1	oxide					214:218	Chitosan-graphene oxide	196:218	Chitosan-graphene oxide green composite films	196:240	Chitosan-graphene oxide green composite films were fabricated by mixing aqueous solution of chitosan and GO using dilute acetic acid as a solvent for chitosan.
27112859	9	93	theme	morphological	1574:1586	arg1	having					1640:1645	having	1640:1645	having dense structure	1640:1661	The morphological analysis of the composites sheets by SEM was smooth having dense structure and showed excellent interaction, miscibility, compatibility and dispersion of GO with chitosan.
27112859	9	93	theme	morphological	1574:1586	arg1	smooth					1633:1638	smooth	1633:1638	smooth	1633:1638	The morphological analysis of the composites sheets by SEM was smooth having dense structure and showed excellent interaction, miscibility, compatibility and dispersion of GO with chitosan.
27112859	9	93	theme	morphological	1574:1586	arg1	analysis					1588:1595	The morphological analysis	1570:1595	The morphological analysis of the composites sheets by SEM	1570:1627	The morphological analysis of the composites sheets by SEM was smooth having dense structure and showed excellent interaction, miscibility, compatibility and dispersion of GO with chitosan.
27112859	0	94	theme	green	6:10	arg1	films					28:32	Novel green nano composites films	0:32	Novel green nano composites films	0:32	Novel green nano composites films fabricated by indigenously synthesized graphene oxide and chitosan.
24704479	0	0	theme	Poria	109:113	arg1	micromorphology					90:104	chemical micromorphology	81:104	Vibrational microspectroscopic identification of powdered traditional medicines: chemical micromorphology of Poria observed by infrared and Raman microspectroscopy.	0:163	Vibrational microspectroscopic identification of powdered traditional medicines: chemical micromorphology of Poria observed by infrared and Raman microspectroscopy.
24704479	4	1	theme	microscopic	964:974	arg1	characteristics					976:990	microscopic characteristics	964:990	microscopic characteristics	964:990	The chemical micromorphology, i.e., chemical compositions and related physical morphologies, of the drug powder can be profiled objectively and quantitatively by infrared and Raman microspectroscopy, leading to better understanding about the formation mechanisms of microscopic characteristics and more accurate identification criteria.
24704479	4	2	theme	identification	1010:1023	arg1	criteria					1025:1032	more accurate identification criteria	996:1032	more accurate identification criteria	996:1032	The chemical micromorphology, i.e., chemical compositions and related physical morphologies, of the drug powder can be profiled objectively and quantitatively by infrared and Raman microspectroscopy, leading to better understanding about the formation mechanisms of microscopic characteristics and more accurate identification criteria.
24704479	1	3	theme	optical	198:204	arg1	microscopes					206:216	optical microscopes	198:216	optical microscopes	198:216	Microscopic identification using optical microscopes is a simple and effective method to identify powdered traditional medicines made from plants, animals and fungi.
24704479	9	4	theme	spherical	1550:1558	arg1	particles					1560:1568	The newfound spherical particles	1537:1568	The newfound spherical particles	1537:1568	The newfound spherical particles may originate from the exuded droplets in the fresh Poria because they are both composed of α-D-glucan.
24704479	8	5	theme	mature	1497:1502	arg1	hyphae					1504:1509	the mature hyphae	1493:1509	the mature hyphae	1493:1509	The granular and branched clumps observed by the optical microscope may be formed from the aggregation of the mature hyphae with β-D-glucan reserves.
24704479	4	6	dep	compositions	743:754	arg1	i.e.					728:731	i.e.	728:731	i.e.	728:731	The chemical micromorphology, i.e., chemical compositions and related physical morphologies, of the drug powder can be profiled objectively and quantitatively by infrared and Raman microspectroscopy, leading to better understanding about the formation mechanisms of microscopic characteristics and more accurate identification criteria.
24704479	8	7	theme	granular	1391:1398	arg1	clumps					1413:1418	The granular and branched clumps	1387:1418	The granular and branched clumps observed by the optical microscope	1387:1453	The granular and branched clumps observed by the optical microscope may be formed from the aggregation of the mature hyphae with β-D-glucan reserves.
24704479	5	8	theme	Chinese	1113:1119	arg1	medicines					1121:1129	the most used traditional Chinese medicines	1087:1129	the most used traditional Chinese medicines	1087:1129	As an example, the powder of Poria, which is one of the most used traditional Chinese medicines, is studied in this research.
24704479	3	9	theme	identification	596:609	arg1	method					611:616	the vibrational microspectroscopic identification method	561:616	the vibrational microspectroscopic identification method	561:616	In this research, the vibrational microspectroscopic identification method is proposed for more explicit discrimination of powdered traditional medicines.
24704479	6	10	from	cm	1275:1276	arg1	features					1235:1242	their infrared spectral features	1211:1242	their infrared spectral features in the region from 1200 to 900 cm(-1)	1211:1280	Three types of hyphae are classified according to their infrared spectral features in the region from 1200 to 900 cm(-1).
24704479	6	10	from	cm	1275:1276	arg1	region					1251:1256	the region	1247:1256	the region from 1200 to 900 cm(-1)	1247:1280	Three types of hyphae are classified according to their infrared spectral features in the region from 1200 to 900 cm(-1).
24704479	4	11	theme	characteristics	976:990	arg1	mechanisms					950:959	the formation mechanisms	936:959	the formation mechanisms of microscopic characteristics and more accurate identification criteria	936:1032	The chemical micromorphology, i.e., chemical compositions and related physical morphologies, of the drug powder can be profiled objectively and quantitatively by infrared and Raman microspectroscopy, leading to better understanding about the formation mechanisms of microscopic characteristics and more accurate identification criteria.
24704479	10	12	from	formation	1750:1758	arg1	Poria					1789:1793	Poria	1789:1793	Poria	1789:1793	The results are helpful to understand the development of the hyphae and the formation of active polysaccharides in Poria and to establish accurate microspectroscopic identification criteria.
24704479	2	13	theme	microscopic	391:401	arg1	characteristics					403:417	the microscopic characteristics	387:417	the microscopic characteristics of drug powder	387:432	Sometimes, the criteria based on physical properties of the microscopic characteristics of drug powder may be ambiguous, which makes the microscopic identification method subjective and empirical to some extent.
24704479	4	14	theme	drug	798:801	arg1	powder					803:808	the drug powder	794:808	the drug powder	794:808	The chemical micromorphology, i.e., chemical compositions and related physical morphologies, of the drug powder can be profiled objectively and quantitatively by infrared and Raman microspectroscopy, leading to better understanding about the formation mechanisms of microscopic characteristics and more accurate identification criteria.
24704479	10	15	from	Poria	1789:1793	arg1	development					1716:1726	the development	1712:1726	the development of the hyphae	1712:1740	The results are helpful to understand the development of the hyphae and the formation of active polysaccharides in Poria and to establish accurate microspectroscopic identification criteria.
24704479	10	15	from	Poria	1789:1793	arg1	formation					1750:1758	the formation	1746:1758	the formation of active polysaccharides in Poria	1746:1793	The results are helpful to understand the development of the hyphae and the formation of active polysaccharides in Poria and to establish accurate microspectroscopic identification criteria.
24704479	3	16	theme	vibrational	565:575	arg1	method					611:616	the vibrational microspectroscopic identification method	561:616	the vibrational microspectroscopic identification method	561:616	In this research, the vibrational microspectroscopic identification method is proposed for more explicit discrimination of powdered traditional medicines.
24704479	2	17	theme	powder	427:432	arg1	characteristics					403:417	the microscopic characteristics	387:417	the microscopic characteristics of drug powder	387:432	Sometimes, the criteria based on physical properties of the microscopic characteristics of drug powder may be ambiguous, which makes the microscopic identification method subjective and empirical to some extent.
24704479	4	18	theme	chemical	734:741	arg1	compositions					743:754	chemical compositions	734:754	chemical compositions	734:754	The chemical micromorphology, i.e., chemical compositions and related physical morphologies, of the drug powder can be profiled objectively and quantitatively by infrared and Raman microspectroscopy, leading to better understanding about the formation mechanisms of microscopic characteristics and more accurate identification criteria.
24704479	0	19	theme	medicines	70:78	arg1	identification					31:44	Vibrational microspectroscopic identification	0:44	Vibrational microspectroscopic identification of powdered traditional medicines: chemical micromorphology of Poria observed by infrared and Raman microspectroscopy.	0:163	Vibrational microspectroscopic identification of powdered traditional medicines: chemical micromorphology of Poria observed by infrared and Raman microspectroscopy.
24704479	9	20	dep	composed	1650:1657	arg1	both					1645:1648	both	1645:1648	both	1645:1648	The newfound spherical particles may originate from the exuded droplets in the fresh Poria because they are both composed of α-D-glucan.
24704479	0	21	theme	Vibrational	0:10	arg1	identification					31:44	Vibrational microspectroscopic identification	0:44	Vibrational microspectroscopic identification of powdered traditional medicines: chemical micromorphology of Poria observed by infrared and Raman microspectroscopy.	0:163	Vibrational microspectroscopic identification of powdered traditional medicines: chemical micromorphology of Poria observed by infrared and Raman microspectroscopy.
24704479	10	22	theme	active	1763:1768	arg1	polysaccharides					1770:1784	active polysaccharides	1763:1784	active polysaccharides in Poria	1763:1793	The results are helpful to understand the development of the hyphae and the formation of active polysaccharides in Poria and to establish accurate microspectroscopic identification criteria.
24704479	0	23	theme	microspectroscopic	12:29	arg1	identification					31:44	Vibrational microspectroscopic identification	0:44	Vibrational microspectroscopic identification of powdered traditional medicines: chemical micromorphology of Poria observed by infrared and Raman microspectroscopy.	0:163	Vibrational microspectroscopic identification of powdered traditional medicines: chemical micromorphology of Poria observed by infrared and Raman microspectroscopy.
24704479	9	24	theme	fresh	1616:1620	arg1	Poria					1622:1626	the fresh Poria	1612:1626	the fresh Poria	1612:1626	The newfound spherical particles may originate from the exuded droplets in the fresh Poria because they are both composed of α-D-glucan.
24704479	1	25	theme	simple	223:228	arg1	method					244:249	a simple and effective method	221:249	a simple and effective method to identify powdered traditional medicines made from plants, animals and fungi	221:328	Microscopic identification using optical microscopes is a simple and effective method to identify powdered traditional medicines made from plants, animals and fungi.
24704479	1	25	theme	simple	223:228	arg1	identification					177:190	Microscopic identification	165:190	Microscopic identification using optical microscopes	165:216	Microscopic identification using optical microscopes is a simple and effective method to identify powdered traditional medicines made from plants, animals and fungi.
24704479	2	26	theme	identification	480:493	arg1	method					495:500	the microscopic identification method	464:500	the microscopic identification method subjective and empirical	464:525	Sometimes, the criteria based on physical properties of the microscopic characteristics of drug powder may be ambiguous, which makes the microscopic identification method subjective and empirical to some extent.
24704479	4	27	theme	formation	940:948	arg1	mechanisms					950:959	the formation mechanisms	936:959	the formation mechanisms of microscopic characteristics and more accurate identification criteria	936:1032	The chemical micromorphology, i.e., chemical compositions and related physical morphologies, of the drug powder can be profiled objectively and quantitatively by infrared and Raman microspectroscopy, leading to better understanding about the formation mechanisms of microscopic characteristics and more accurate identification criteria.
24704479	10	28	theme	hyphae	1735:1740	arg1	development					1716:1726	the development	1712:1726	the development of the hyphae	1712:1740	The results are helpful to understand the development of the hyphae and the formation of active polysaccharides in Poria and to establish accurate microspectroscopic identification criteria.
24704479	10	28	theme	hyphae	1735:1740	arg1	formation					1750:1758	the formation	1746:1758	the formation of active polysaccharides in Poria	1746:1793	The results are helpful to understand the development of the hyphae and the formation of active polysaccharides in Poria and to establish accurate microspectroscopic identification criteria.
24704479	10	29	theme	identification	1840:1853	arg1	criteria					1855:1862	accurate microspectroscopic identification criteria	1812:1862	accurate microspectroscopic identification criteria	1812:1862	The results are helpful to understand the development of the hyphae and the formation of active polysaccharides in Poria and to establish accurate microspectroscopic identification criteria.
24704479	8	30	theme	branched	1404:1411	arg1	clumps					1413:1418	The granular and branched clumps	1387:1418	The granular and branched clumps observed by the optical microscope	1387:1453	The granular and branched clumps observed by the optical microscope may be formed from the aggregation of the mature hyphae with β-D-glucan reserves.
24704479	2	31	theme	drug	422:425	arg1	powder					427:432	drug powder	422:432	drug powder	422:432	Sometimes, the criteria based on physical properties of the microscopic characteristics of drug powder may be ambiguous, which makes the microscopic identification method subjective and empirical to some extent.
24704479	2	32	theme	microscopic	468:478	arg1	method					495:500	the microscopic identification method	464:500	the microscopic identification method subjective and empirical	464:525	Sometimes, the criteria based on physical properties of the microscopic characteristics of drug powder may be ambiguous, which makes the microscopic identification method subjective and empirical to some extent.
24704479	5	33	theme	medicines	1121:1129	arg1	powder					1054:1059	the powder	1050:1059	the powder	1050:1059	As an example, the powder of Poria, which is one of the most used traditional Chinese medicines, is studied in this research.
24704479	5	33	theme	medicines	1121:1129	arg1	one					1080:1082	one	1080:1082	one	1080:1082	As an example, the powder of Poria, which is one of the most used traditional Chinese medicines, is studied in this research.
24704479	5	33	theme	medicines	1121:1129	arg1	medicines					1121:1129	the most used traditional Chinese medicines	1087:1129	the most used traditional Chinese medicines	1087:1129	As an example, the powder of Poria, which is one of the most used traditional Chinese medicines, is studied in this research.
24704479	6	34	theme	spectral	1226:1233	arg1	features					1235:1242	their infrared spectral features	1211:1242	their infrared spectral features in the region from 1200 to 900 cm(-1)	1211:1280	Three types of hyphae are classified according to their infrared spectral features in the region from 1200 to 900 cm(-1).
24704479	1	35	theme	effective	234:242	arg1	method					244:249	a simple and effective method	221:249	a simple and effective method to identify powdered traditional medicines made from plants, animals and fungi	221:328	Microscopic identification using optical microscopes is a simple and effective method to identify powdered traditional medicines made from plants, animals and fungi.
24704479	1	35	theme	effective	234:242	arg1	identification					177:190	Microscopic identification	165:190	Microscopic identification using optical microscopes	165:216	Microscopic identification using optical microscopes is a simple and effective method to identify powdered traditional medicines made from plants, animals and fungi.
24704479	10	36	dep	helpful	1690:1696	arg1	understand					1701:1710	understand	1701:1710	to understand the development of the hyphae and the formation of active polysaccharides in Poria	1698:1793	The results are helpful to understand the development of the hyphae and the formation of active polysaccharides in Poria and to establish accurate microspectroscopic identification criteria.
24704479	10	36	dep	helpful	1690:1696	arg1	helpful					1690:1696	helpful	1690:1696	helpful	1690:1696	The results are helpful to understand the development of the hyphae and the formation of active polysaccharides in Poria and to establish accurate microspectroscopic identification criteria.
24704479	10	36	dep	helpful	1690:1696	arg1	results					1678:1684	The results	1674:1684	The results	1674:1684	The results are helpful to understand the development of the hyphae and the formation of active polysaccharides in Poria and to establish accurate microspectroscopic identification criteria.
24704479	10	36	dep	helpful	1690:1696	arg1	establish					1802:1810	establish	1802:1810	to establish accurate microspectroscopic identification criteria	1799:1862	The results are helpful to understand the development of the hyphae and the formation of active polysaccharides in Poria and to establish accurate microspectroscopic identification criteria.
24704479	0	37	theme	Raman	140:144	arg1	microspectroscopy					146:162	Raman microspectroscopy	140:162	Raman microspectroscopy	140:162	Vibrational microspectroscopic identification of powdered traditional medicines: chemical micromorphology of Poria observed by infrared and Raman microspectroscopy.
24704479	8	38	theme	optical	1436:1442	arg1	microscope					1444:1453	the optical microscope	1432:1453	the optical microscope	1432:1453	The granular and branched clumps observed by the optical microscope may be formed from the aggregation of the mature hyphae with β-D-glucan reserves.
24704479	6	39	theme	infrared	1217:1224	arg1	features					1235:1242	their infrared spectral features	1211:1242	their infrared spectral features in the region from 1200 to 900 cm(-1)	1211:1280	Three types of hyphae are classified according to their infrared spectral features in the region from 1200 to 900 cm(-1).
24704479	4	40	theme	chemical	702:709	arg1	micromorphology					711:725	The chemical micromorphology	698:725	The chemical micromorphology	698:725	The chemical micromorphology, i.e., chemical compositions and related physical morphologies, of the drug powder can be profiled objectively and quantitatively by infrared and Raman microspectroscopy, leading to better understanding about the formation mechanisms of microscopic characteristics and more accurate identification criteria.
24704479	0	41	theme	traditional	58:68	arg1	medicines					70:78	powdered traditional medicines	49:78	powdered traditional medicines	49:78	Vibrational microspectroscopic identification of powdered traditional medicines: chemical micromorphology of Poria observed by infrared and Raman microspectroscopy.
24704479	5	42	theme	Poria	1064:1068	arg1	powder					1054:1059	the powder	1050:1059	the powder	1050:1059	As an example, the powder of Poria, which is one of the most used traditional Chinese medicines, is studied in this research.
24704479	5	42	theme	Poria	1064:1068	arg1	one					1080:1082	one	1080:1082	one	1080:1082	As an example, the powder of Poria, which is one of the most used traditional Chinese medicines, is studied in this research.
24704479	5	42	theme	Poria	1064:1068	arg1	medicines					1121:1129	the most used traditional Chinese medicines	1087:1129	the most used traditional Chinese medicines	1087:1129	As an example, the powder of Poria, which is one of the most used traditional Chinese medicines, is studied in this research.
24704479	5	42	theme	Poria	1064:1068	arg1	example					1041:1047	an example	1038:1047	an example	1038:1047	As an example, the powder of Poria, which is one of the most used traditional Chinese medicines, is studied in this research.
24704479	9	43	theme	newfound	1541:1548	arg1	particles					1560:1568	The newfound spherical particles	1537:1568	The newfound spherical particles	1537:1568	The newfound spherical particles may originate from the exuded droplets in the fresh Poria because they are both composed of α-D-glucan.
24704479	7	44	theme	polysaccharides	1302:1316	arg1	kinds					1293:1297	Different kinds	1283:1297	Different kinds of polysaccharides	1283:1316	Different kinds of polysaccharides indicate that these hyphae may be in different stages of the growth.
24704479	5	45	theme	used	1096:1099	arg1	medicines					1121:1129	the most used traditional Chinese medicines	1087:1129	the most used traditional Chinese medicines	1087:1129	As an example, the powder of Poria, which is one of the most used traditional Chinese medicines, is studied in this research.
24704479	6	46	from	features	1235:1242	arg1	cm					1275:1276	1200 to 900 cm	1263:1276	1200 to 900 cm(-1)	1263:1280	Three types of hyphae are classified according to their infrared spectral features in the region from 1200 to 900 cm(-1).
24704479	6	46	from	features	1235:1242	arg1	region					1251:1256	the region	1247:1256	the region from 1200 to 900 cm(-1)	1247:1280	Three types of hyphae are classified according to their infrared spectral features in the region from 1200 to 900 cm(-1).
24704479	6	46	from	features	1235:1242	arg1	-1					1278:1279	-1	1278:1279	-1	1278:1279	Three types of hyphae are classified according to their infrared spectral features in the region from 1200 to 900 cm(-1).
24704479	0	47	theme	powdered	49:56	arg1	medicines					70:78	powdered traditional medicines	49:78	powdered traditional medicines	49:78	Vibrational microspectroscopic identification of powdered traditional medicines: chemical micromorphology of Poria observed by infrared and Raman microspectroscopy.
24704479	8	48	with	aggregation	1478:1488	arg1	reserves					1527:1534	β-D-glucan reserves	1516:1534	β-D-glucan reserves	1516:1534	The granular and branched clumps observed by the optical microscope may be formed from the aggregation of the mature hyphae with β-D-glucan reserves.
24704479	2	49	theme	empirical	517:525	arg1	method					495:500	the microscopic identification method	464:500	the microscopic identification method subjective and empirical	464:525	Sometimes, the criteria based on physical properties of the microscopic characteristics of drug powder may be ambiguous, which makes the microscopic identification method subjective and empirical to some extent.
24704479	7	50	theme	growth	1379:1384	arg1	stages					1365:1370	different stages	1355:1370	different stages of the growth	1355:1384	Different kinds of polysaccharides indicate that these hyphae may be in different stages of the growth.
24704479	3	51	theme	powdered	666:673	arg1	medicines					687:695	powdered traditional medicines	666:695	powdered traditional medicines	666:695	In this research, the vibrational microspectroscopic identification method is proposed for more explicit discrimination of powdered traditional medicines.
24704479	10	52	theme	polysaccharides	1770:1784	arg1	development					1716:1726	the development	1712:1726	the development of the hyphae	1712:1740	The results are helpful to understand the development of the hyphae and the formation of active polysaccharides in Poria and to establish accurate microspectroscopic identification criteria.
24704479	10	52	theme	polysaccharides	1770:1784	arg1	formation					1750:1758	the formation	1746:1758	the formation of active polysaccharides in Poria	1746:1793	The results are helpful to understand the development of the hyphae and the formation of active polysaccharides in Poria and to establish accurate microspectroscopic identification criteria.
24704479	4	53	theme	accurate	1001:1008	arg1	criteria					1025:1032	more accurate identification criteria	996:1032	more accurate identification criteria	996:1032	The chemical micromorphology, i.e., chemical compositions and related physical morphologies, of the drug powder can be profiled objectively and quantitatively by infrared and Raman microspectroscopy, leading to better understanding about the formation mechanisms of microscopic characteristics and more accurate identification criteria.
24704479	7	54	theme	Different	1283:1291	arg1	kinds					1293:1297	Different kinds	1283:1297	Different kinds of polysaccharides	1283:1316	Different kinds of polysaccharides indicate that these hyphae may be in different stages of the growth.
24704479	3	55	theme	explicit	639:646	arg1	discrimination					648:661	more explicit discrimination	634:661	more explicit discrimination of powdered traditional medicines	634:695	In this research, the vibrational microspectroscopic identification method is proposed for more explicit discrimination of powdered traditional medicines.
24704479	6	56	dep	900	1271:1273	arg1	to					1268:1269	to	1268:1269	to	1268:1269	Three types of hyphae are classified according to their infrared spectral features in the region from 1200 to 900 cm(-1).
24704479	2	57	theme	subjective	502:511	arg1	method					495:500	the microscopic identification method	464:500	the microscopic identification method subjective and empirical	464:525	Sometimes, the criteria based on physical properties of the microscopic characteristics of drug powder may be ambiguous, which makes the microscopic identification method subjective and empirical to some extent.
24704479	8	58	theme	β-D-glucan	1516:1525	arg1	reserves					1527:1534	β-D-glucan reserves	1516:1534	β-D-glucan reserves	1516:1534	The granular and branched clumps observed by the optical microscope may be formed from the aggregation of the mature hyphae with β-D-glucan reserves.
24704479	4	59	theme	better	909:914	arg1	understanding					916:928	better understanding	909:928	better understanding about the formation mechanisms of microscopic characteristics and more accurate identification criteria	909:1032	The chemical micromorphology, i.e., chemical compositions and related physical morphologies, of the drug powder can be profiled objectively and quantitatively by infrared and Raman microspectroscopy, leading to better understanding about the formation mechanisms of microscopic characteristics and more accurate identification criteria.
24704479	4	60	theme	powder	803:808	arg1	micromorphology					711:725	The chemical micromorphology	698:725	The chemical micromorphology	698:725	The chemical micromorphology, i.e., chemical compositions and related physical morphologies, of the drug powder can be profiled objectively and quantitatively by infrared and Raman microspectroscopy, leading to better understanding about the formation mechanisms of microscopic characteristics and more accurate identification criteria.
24704479	10	61	theme	accurate	1812:1819	arg1	criteria					1855:1862	accurate microspectroscopic identification criteria	1812:1862	accurate microspectroscopic identification criteria	1812:1862	The results are helpful to understand the development of the hyphae and the formation of active polysaccharides in Poria and to establish accurate microspectroscopic identification criteria.
24704479	10	62	from	polysaccharides	1770:1784	arg1	Poria					1789:1793	Poria	1789:1793	Poria	1789:1793	The results are helpful to understand the development of the hyphae and the formation of active polysaccharides in Poria and to establish accurate microspectroscopic identification criteria.
24704479	4	63	theme	criteria	1025:1032	arg1	mechanisms					950:959	the formation mechanisms	936:959	the formation mechanisms of microscopic characteristics and more accurate identification criteria	936:1032	The chemical micromorphology, i.e., chemical compositions and related physical morphologies, of the drug powder can be profiled objectively and quantitatively by infrared and Raman microspectroscopy, leading to better understanding about the formation mechanisms of microscopic characteristics and more accurate identification criteria.
24704479	5	64	theme	traditional	1101:1111	arg1	medicines					1121:1129	the most used traditional Chinese medicines	1087:1129	the most used traditional Chinese medicines	1087:1129	As an example, the powder of Poria, which is one of the most used traditional Chinese medicines, is studied in this research.
24704479	4	65	theme	Raman	873:877	arg1	microspectroscopy					879:895	Raman microspectroscopy	873:895	Raman microspectroscopy	873:895	The chemical micromorphology, i.e., chemical compositions and related physical morphologies, of the drug powder can be profiled objectively and quantitatively by infrared and Raman microspectroscopy, leading to better understanding about the formation mechanisms of microscopic characteristics and more accurate identification criteria.
24704479	4	66	theme	related	760:766	arg1	morphologies					777:788	related physical morphologies	760:788	related physical morphologies	760:788	The chemical micromorphology, i.e., chemical compositions and related physical morphologies, of the drug powder can be profiled objectively and quantitatively by infrared and Raman microspectroscopy, leading to better understanding about the formation mechanisms of microscopic characteristics and more accurate identification criteria.
24704479	2	67	theme	physical	364:371	arg1	properties					373:382	physical properties	364:382	physical properties of the microscopic characteristics of drug powder	364:432	Sometimes, the criteria based on physical properties of the microscopic characteristics of drug powder may be ambiguous, which makes the microscopic identification method subjective and empirical to some extent.
24704479	10	68	theme	microspectroscopic	1821:1838	arg1	criteria					1855:1862	accurate microspectroscopic identification criteria	1812:1862	accurate microspectroscopic identification criteria	1812:1862	The results are helpful to understand the development of the hyphae and the formation of active polysaccharides in Poria and to establish accurate microspectroscopic identification criteria.
24704479	1	69	theme	powdered	263:270	arg1	medicines					284:292	powdered traditional medicines	263:292	powdered traditional medicines made from plants, animals and fungi	263:328	Microscopic identification using optical microscopes is a simple and effective method to identify powdered traditional medicines made from plants, animals and fungi.
24704479	3	70	theme	microspectroscopic	577:594	arg1	method					611:616	the vibrational microspectroscopic identification method	561:616	the vibrational microspectroscopic identification method	561:616	In this research, the vibrational microspectroscopic identification method is proposed for more explicit discrimination of powdered traditional medicines.
24704479	9	71	theme	exuded	1593:1598	arg1	droplets					1600:1607	the exuded droplets	1589:1607	the exuded droplets in the fresh Poria	1589:1626	The newfound spherical particles may originate from the exuded droplets in the fresh Poria because they are both composed of α-D-glucan.
24704479	0	72	dep	identification	31:44	arg1	micromorphology					90:104	chemical micromorphology	81:104	Vibrational microspectroscopic identification of powdered traditional medicines: chemical micromorphology of Poria observed by infrared and Raman microspectroscopy.	0:163	Vibrational microspectroscopic identification of powdered traditional medicines: chemical micromorphology of Poria observed by infrared and Raman microspectroscopy.
24704479	10	73	from	development	1716:1726	arg1	Poria					1789:1793	Poria	1789:1793	Poria	1789:1793	The results are helpful to understand the development of the hyphae and the formation of active polysaccharides in Poria and to establish accurate microspectroscopic identification criteria.
24704479	1	74	theme	Microscopic	165:175	arg1	method					244:249	a simple and effective method	221:249	a simple and effective method to identify powdered traditional medicines made from plants, animals and fungi	221:328	Microscopic identification using optical microscopes is a simple and effective method to identify powdered traditional medicines made from plants, animals and fungi.
24704479	1	74	theme	Microscopic	165:175	arg1	identification					177:190	Microscopic identification	165:190	Microscopic identification using optical microscopes	165:216	Microscopic identification using optical microscopes is a simple and effective method to identify powdered traditional medicines made from plants, animals and fungi.
24704479	1	75	theme	traditional	272:282	arg1	medicines					284:292	powdered traditional medicines	263:292	powdered traditional medicines made from plants, animals and fungi	263:328	Microscopic identification using optical microscopes is a simple and effective method to identify powdered traditional medicines made from plants, animals and fungi.
24704479	8	76	theme	hyphae	1504:1509	arg1	aggregation					1478:1488	the aggregation	1474:1488	the aggregation of the mature hyphae with β-D-glucan reserves	1474:1534	The granular and branched clumps observed by the optical microscope may be formed from the aggregation of the mature hyphae with β-D-glucan reserves.
24704479	6	77	theme	hyphae	1176:1181	arg1	types					1167:1171	Three types	1161:1171	Three types of hyphae	1161:1181	Three types of hyphae are classified according to their infrared spectral features in the region from 1200 to 900 cm(-1).
24704479	3	78	theme	traditional	675:685	arg1	medicines					687:695	powdered traditional medicines	666:695	powdered traditional medicines	666:695	In this research, the vibrational microspectroscopic identification method is proposed for more explicit discrimination of powdered traditional medicines.
24704479	7	79	theme	different	1355:1363	arg1	stages					1365:1370	different stages	1355:1370	different stages of the growth	1355:1384	Different kinds of polysaccharides indicate that these hyphae may be in different stages of the growth.
24704479	4	80	theme	physical	768:775	arg1	morphologies					777:788	related physical morphologies	760:788	related physical morphologies	760:788	The chemical micromorphology, i.e., chemical compositions and related physical morphologies, of the drug powder can be profiled objectively and quantitatively by infrared and Raman microspectroscopy, leading to better understanding about the formation mechanisms of microscopic characteristics and more accurate identification criteria.
24704479	9	81	from	droplets	1600:1607	arg1	Poria					1622:1626	the fresh Poria	1612:1626	the fresh Poria	1612:1626	The newfound spherical particles may originate from the exuded droplets in the fresh Poria because they are both composed of α-D-glucan.
24704479	0	82	theme	chemical	81:88	arg1	micromorphology					90:104	chemical micromorphology	81:104	Vibrational microspectroscopic identification of powdered traditional medicines: chemical micromorphology of Poria observed by infrared and Raman microspectroscopy.	0:163	Vibrational microspectroscopic identification of powdered traditional medicines: chemical micromorphology of Poria observed by infrared and Raman microspectroscopy.
24704479	3	83	theme	medicines	687:695	arg1	discrimination					648:661	more explicit discrimination	634:661	more explicit discrimination of powdered traditional medicines	634:695	In this research, the vibrational microspectroscopic identification method is proposed for more explicit discrimination of powdered traditional medicines.
24704479	2	84	theme	characteristics	403:417	arg1	properties					373:382	physical properties	364:382	physical properties of the microscopic characteristics of drug powder	364:432	Sometimes, the criteria based on physical properties of the microscopic characteristics of drug powder may be ambiguous, which makes the microscopic identification method subjective and empirical to some extent.
28891420	0	0	theme	polymeric	54:62	arg1	substances					64:73	its extracellular polymeric substances	36:73	its extracellular polymeric substances in sequencing batch biofilm reactors	36:110	Influence of Al(III) on biofilm and its extracellular polymeric substances in sequencing batch biofilm reactors.
28891420	1	1	theme	different	310:318	arg1	phases					327:332	different growth phases	310:332	different growth phases	310:332	This paper presented the influence of Al(III) on biodegradability, micromorphology, composition and functional groups characteristics of the biofilm extracellular polymeric substances (EPS) during different growth phases.
28891420	4	2	from	phases	788:793	arg1	promotion					747:755	promotion	747:755	promotion of the EPS secretion in growth phases of the biofilm	747:808	The micromorphological observation indicated that Al(III) led to a reduction of the filaments and promotion of the EPS secretion in growth phases of the biofilm, also Al(III) could promote microorganisms to form larger colonies for mature biofilm.
28891420	4	2	from	phases	788:793	arg1	reduction					717:725	a reduction	715:725	a reduction of the filaments	715:741	The micromorphological observation indicated that Al(III) led to a reduction of the filaments and promotion of the EPS secretion in growth phases of the biofilm, also Al(III) could promote microorganisms to form larger colonies for mature biofilm.
28891420	1	3	theme	functional	213:222	arg1	groups					224:229	functional groups	213:229	functional groups	213:229	This paper presented the influence of Al(III) on biodegradability, micromorphology, composition and functional groups characteristics of the biofilm extracellular polymeric substances (EPS) during different growth phases.
28891420	0	4	theme	extracellular	40:52	arg1	substances					64:73	its extracellular polymeric substances	36:73	its extracellular polymeric substances in sequencing batch biofilm reactors	36:110	Influence of Al(III) on biofilm and its extracellular polymeric substances in sequencing batch biofilm reactors.
28891420	1	5	theme	growth	320:325	arg1	phases					327:332	different growth phases	310:332	different growth phases	310:332	This paper presented the influence of Al(III) on biodegradability, micromorphology, composition and functional groups characteristics of the biofilm extracellular polymeric substances (EPS) during different growth phases.
28891420	4	6	from	reduction	717:725	arg1	phases					788:793	growth phases	781:793	growth phases of the biofilm	781:808	The micromorphological observation indicated that Al(III) led to a reduction of the filaments and promotion of the EPS secretion in growth phases of the biofilm, also Al(III) could promote microorganisms to form larger colonies for mature biofilm.
28891420	4	7	dep	led	708:710	arg1	III					703:705	III	703:705	III	703:705	The micromorphological observation indicated that Al(III) led to a reduction of the filaments and promotion of the EPS secretion in growth phases of the biofilm, also Al(III) could promote microorganisms to form larger colonies for mature biofilm.
28891420	4	7	dep	led	708:710	arg1	promote					830:836	promote	830:836	(III) could promote microorganisms to form larger colonies for mature biofilm	818:894	The micromorphological observation indicated that Al(III) led to a reduction of the filaments and promotion of the EPS secretion in growth phases of the biofilm, also Al(III) could promote microorganisms to form larger colonies for mature biofilm.
28891420	1	8	theme	groups	224:229	arg1	characteristics					231:245	biodegradability, micromorphology, composition and functional groups characteristics	162:245	biodegradability, micromorphology, composition and functional groups characteristics of the biofilm extracellular polymeric substances (EPS)	162:301	This paper presented the influence of Al(III) on biodegradability, micromorphology, composition and functional groups characteristics of the biofilm extracellular polymeric substances (EPS) during different growth phases.
28891420	0	9	from	Influence	0:8	arg1	substances					64:73	its extracellular polymeric substances	36:73	its extracellular polymeric substances in sequencing batch biofilm reactors	36:110	Influence of Al(III) on biofilm and its extracellular polymeric substances in sequencing batch biofilm reactors.
28891420	0	9	from	Influence	0:8	arg1	biofilm					24:30	biofilm	24:30	biofilm	24:30	Influence of Al(III) on biofilm and its extracellular polymeric substances in sequencing batch biofilm reactors.
28891420	1	10	from	influence	138:146	arg1	characteristics					231:245	biodegradability, micromorphology, composition and functional groups characteristics	162:245	biodegradability, micromorphology, composition and functional groups characteristics of the biofilm extracellular polymeric substances (EPS)	162:301	This paper presented the influence of Al(III) on biodegradability, micromorphology, composition and functional groups characteristics of the biofilm extracellular polymeric substances (EPS) during different growth phases.
28891420	5	11	theme	biofilm	1123:1129	arg1	growth					1131:1136	biofilm growth	1123:1136	biofilm growth	1123:1136	Then, the analysis of EPS contents and components suggested that Al(III) could increase the protein (PN) of tightly bound EPS (TB-EPS) which alleviated the metal toxicity inhibition on the biofilm during the initial phases of biofilm growth.
28891420	4	12	dep	promote	830:836	arg1	III					819:821	III	819:821	III	819:821	The micromorphological observation indicated that Al(III) led to a reduction of the filaments and promotion of the EPS secretion in growth phases of the biofilm, also Al(III) could promote microorganisms to form larger colonies for mature biofilm.
28891420	0	13	from	biofilm	24:30	arg1	reactors					103:110	sequencing batch biofilm reactors	78:110	sequencing batch biofilm reactors	78:110	Influence of Al(III) on biofilm and its extracellular polymeric substances in sequencing batch biofilm reactors.
28891420	4	14	theme	biofilm	802:808	arg1	phases					788:793	growth phases	781:793	growth phases of the biofilm	781:808	The micromorphological observation indicated that Al(III) led to a reduction of the filaments and promotion of the EPS secretion in growth phases of the biofilm, also Al(III) could promote microorganisms to form larger colonies for mature biofilm.
28891420	7	15	theme	TB-EPS	1385:1390	arg1	groups					1437:1442	the functional groups	1422:1442	the functional groups of protein and polysaccharides	1422:1473	Finally, through the Fourier transform infrared spectroscopy, it was found that Al(III) was beneficial for the proliferation and secretion of TB-EPS functional groups, especially the functional groups of protein and polysaccharides.
28891420	7	15	theme	TB-EPS	1385:1390	arg1	groups					1403:1408	TB-EPS functional groups	1385:1408	TB-EPS functional groups	1385:1408	Finally, through the Fourier transform infrared spectroscopy, it was found that Al(III) was beneficial for the proliferation and secretion of TB-EPS functional groups, especially the functional groups of protein and polysaccharides.
28891420	7	15	theme	TB-EPS	1385:1390	arg1	polysaccharides					1459:1473	polysaccharides	1459:1473	polysaccharides	1459:1473	Finally, through the Fourier transform infrared spectroscopy, it was found that Al(III) was beneficial for the proliferation and secretion of TB-EPS functional groups, especially the functional groups of protein and polysaccharides.
28891420	7	15	theme	TB-EPS	1385:1390	arg1	protein					1447:1453	protein	1447:1453	protein	1447:1453	Finally, through the Fourier transform infrared spectroscopy, it was found that Al(III) was beneficial for the proliferation and secretion of TB-EPS functional groups, especially the functional groups of protein and polysaccharides.
28891420	0	16	theme	batch	89:93	arg1	reactors					103:110	sequencing batch biofilm reactors	78:110	sequencing batch biofilm reactors	78:110	Influence of Al(III) on biofilm and its extracellular polymeric substances in sequencing batch biofilm reactors.
28891420	3	17	theme	biofilm	488:494	arg1	development					496:506	biofilm development	488:506	biofilm development	488:506	The results elucidated that Al(III) affected biofilm development adversely at the beginning of biofilm growth, but promoted the biofilm mass and improved the biofilm activity with the growth of the biofilm.
28891420	0	18	theme	sequencing	78:87	arg1	reactors					103:110	sequencing batch biofilm reactors	78:110	sequencing batch biofilm reactors	78:110	Influence of Al(III) on biofilm and its extracellular polymeric substances in sequencing batch biofilm reactors.
28891420	4	19	theme	secretion	768:776	arg1	promotion					747:755	promotion	747:755	promotion of the EPS secretion in growth phases of the biofilm	747:808	The micromorphological observation indicated that Al(III) led to a reduction of the filaments and promotion of the EPS secretion in growth phases of the biofilm, also Al(III) could promote microorganisms to form larger colonies for mature biofilm.
28891420	4	19	theme	secretion	768:776	arg1	reduction					717:725	a reduction	715:725	a reduction of the filaments	715:741	The micromorphological observation indicated that Al(III) led to a reduction of the filaments and promotion of the EPS secretion in growth phases of the biofilm, also Al(III) could promote microorganisms to form larger colonies for mature biofilm.
28891420	1	20	theme	Al	151:152	arg1	influence					138:146	the influence	134:146	the influence of Al(III) on biodegradability, micromorphology, composition and functional groups characteristics of the biofilm extracellular polymeric substances (EPS)	134:301	This paper presented the influence of Al(III) on biodegradability, micromorphology, composition and functional groups characteristics of the biofilm extracellular polymeric substances (EPS) during different growth phases.
28891420	4	21	from	secretion	768:776	arg1	phases					788:793	growth phases	781:793	growth phases of the biofilm	781:808	The micromorphological observation indicated that Al(III) led to a reduction of the filaments and promotion of the EPS secretion in growth phases of the biofilm, also Al(III) could promote microorganisms to form larger colonies for mature biofilm.
28891420	6	22	theme	maturation	1224:1233	arg1	moment					1235:1240	the biofilm maturation moment	1212:1240	the biofilm maturation moment	1212:1240	The biofilm could gradually adapt to the inhibition caused by Al(III) at the biofilm maturation moment.
28891420	7	23	dep	proliferation	1354:1366	arg1	the					1350:1352	the	1350:1352	the	1350:1352	Finally, through the Fourier transform infrared spectroscopy, it was found that Al(III) was beneficial for the proliferation and secretion of TB-EPS functional groups, especially the functional groups of protein and polysaccharides.
28891420	5	24	theme	metal	1053:1057	arg1	toxicity					1059:1066	the metal toxicity	1049:1066	the metal toxicity inhibition	1049:1077	Then, the analysis of EPS contents and components suggested that Al(III) could increase the protein (PN) of tightly bound EPS (TB-EPS) which alleviated the metal toxicity inhibition on the biofilm during the initial phases of biofilm growth.
28891420	4	25	theme	growth	781:786	arg1	phases					788:793	growth phases	781:793	growth phases of the biofilm	781:808	The micromorphological observation indicated that Al(III) led to a reduction of the filaments and promotion of the EPS secretion in growth phases of the biofilm, also Al(III) could promote microorganisms to form larger colonies for mature biofilm.
28891420	7	26	theme	functional	1426:1435	arg1	groups					1437:1442	the functional groups	1422:1442	the functional groups of protein and polysaccharides	1422:1473	Finally, through the Fourier transform infrared spectroscopy, it was found that Al(III) was beneficial for the proliferation and secretion of TB-EPS functional groups, especially the functional groups of protein and polysaccharides.
28891420	7	26	theme	functional	1426:1435	arg1	groups					1403:1408	TB-EPS functional groups	1385:1408	TB-EPS functional groups	1385:1408	Finally, through the Fourier transform infrared spectroscopy, it was found that Al(III) was beneficial for the proliferation and secretion of TB-EPS functional groups, especially the functional groups of protein and polysaccharides.
28891420	7	26	theme	functional	1426:1435	arg1	polysaccharides					1459:1473	polysaccharides	1459:1473	polysaccharides	1459:1473	Finally, through the Fourier transform infrared spectroscopy, it was found that Al(III) was beneficial for the proliferation and secretion of TB-EPS functional groups, especially the functional groups of protein and polysaccharides.
28891420	7	26	theme	functional	1426:1435	arg1	protein					1447:1453	protein	1447:1453	protein	1447:1453	Finally, through the Fourier transform infrared spectroscopy, it was found that Al(III) was beneficial for the proliferation and secretion of TB-EPS functional groups, especially the functional groups of protein and polysaccharides.
28891420	3	27	theme	biofilm	538:544	arg1	growth					546:551	biofilm growth	538:551	biofilm growth	538:551	The results elucidated that Al(III) affected biofilm development adversely at the beginning of biofilm growth, but promoted the biofilm mass and improved the biofilm activity with the growth of the biofilm.
28891420	2	28	theme	biofilm	356:362	arg1	reactors					364:371	The sequencing batch biofilm reactors	335:371	The sequencing batch biofilm reactors	335:371	The sequencing batch biofilm reactors were developed to cultivate biofilms under different Al(III) dosages.
28891420	2	29	theme	Al	426:427	arg1	dosages					434:440	different Al(III) dosages	416:440	different Al(III) dosages	416:440	The sequencing batch biofilm reactors were developed to cultivate biofilms under different Al(III) dosages.
28891420	1	30	theme	biofilm	254:260	arg1	EPS					298:300	EPS	298:300	EPS	298:300	This paper presented the influence of Al(III) on biodegradability, micromorphology, composition and functional groups characteristics of the biofilm extracellular polymeric substances (EPS) during different growth phases.
28891420	1	30	theme	biofilm	254:260	arg1	substances					286:295	the biofilm extracellular polymeric substances	250:295	the biofilm extracellular polymeric substances (EPS)	250:301	This paper presented the influence of Al(III) on biodegradability, micromorphology, composition and functional groups characteristics of the biofilm extracellular polymeric substances (EPS) during different growth phases.
28891420	4	31	theme	EPS	764:766	arg1	secretion					768:776	the EPS secretion	760:776	the EPS secretion in growth phases of the biofilm	760:808	The micromorphological observation indicated that Al(III) led to a reduction of the filaments and promotion of the EPS secretion in growth phases of the biofilm, also Al(III) could promote microorganisms to form larger colonies for mature biofilm.
28891420	0	32	theme	Al	13:14	arg1	Influence					0:8	Influence	0:8	Influence of Al(III) on biofilm and its extracellular polymeric substances in sequencing batch biofilm reactors.	0:111	Influence of Al(III) on biofilm and its extracellular polymeric substances in sequencing batch biofilm reactors.
28891420	1	33	theme	extracellular	262:274	arg1	EPS					298:300	EPS	298:300	EPS	298:300	This paper presented the influence of Al(III) on biodegradability, micromorphology, composition and functional groups characteristics of the biofilm extracellular polymeric substances (EPS) during different growth phases.
28891420	1	33	theme	extracellular	262:274	arg1	substances					286:295	the biofilm extracellular polymeric substances	250:295	the biofilm extracellular polymeric substances (EPS)	250:301	This paper presented the influence of Al(III) on biodegradability, micromorphology, composition and functional groups characteristics of the biofilm extracellular polymeric substances (EPS) during different growth phases.
28891420	0	34	theme	biofilm	95:101	arg1	reactors					103:110	sequencing batch biofilm reactors	78:110	sequencing batch biofilm reactors	78:110	Influence of Al(III) on biofilm and its extracellular polymeric substances in sequencing batch biofilm reactors.
28891420	3	35	theme	biofilm	601:607	arg1	activity					609:616	the biofilm activity	597:616	the biofilm activity	597:616	The results elucidated that Al(III) affected biofilm development adversely at the beginning of biofilm growth, but promoted the biofilm mass and improved the biofilm activity with the growth of the biofilm.
28891420	3	36	dep	affected	479:486	arg1	III					474:476	III	474:476	III	474:476	The results elucidated that Al(III) affected biofilm development adversely at the beginning of biofilm growth, but promoted the biofilm mass and improved the biofilm activity with the growth of the biofilm.
28891420	5	37	theme	contents	923:930	arg1	analysis					907:914	the analysis	903:914	the analysis of EPS contents and components	903:945	Then, the analysis of EPS contents and components suggested that Al(III) could increase the protein (PN) of tightly bound EPS (TB-EPS) which alleviated the metal toxicity inhibition on the biofilm during the initial phases of biofilm growth.
28891420	1	38	theme	polymeric	276:284	arg1	EPS					298:300	EPS	298:300	EPS	298:300	This paper presented the influence of Al(III) on biodegradability, micromorphology, composition and functional groups characteristics of the biofilm extracellular polymeric substances (EPS) during different growth phases.
28891420	1	38	theme	polymeric	276:284	arg1	substances					286:295	the biofilm extracellular polymeric substances	250:295	the biofilm extracellular polymeric substances (EPS)	250:301	This paper presented the influence of Al(III) on biodegradability, micromorphology, composition and functional groups characteristics of the biofilm extracellular polymeric substances (EPS) during different growth phases.
28891420	4	39	theme	filaments	734:741	arg1	promotion					747:755	promotion	747:755	promotion of the EPS secretion in growth phases of the biofilm	747:808	The micromorphological observation indicated that Al(III) led to a reduction of the filaments and promotion of the EPS secretion in growth phases of the biofilm, also Al(III) could promote microorganisms to form larger colonies for mature biofilm.
28891420	4	39	theme	filaments	734:741	arg1	reduction					717:725	a reduction	715:725	a reduction of the filaments	715:741	The micromorphological observation indicated that Al(III) led to a reduction of the filaments and promotion of the EPS secretion in growth phases of the biofilm, also Al(III) could promote microorganisms to form larger colonies for mature biofilm.
28891420	1	40	theme	biodegradability	162:177	arg1	characteristics					231:245	biodegradability, micromorphology, composition and functional groups characteristics	162:245	biodegradability, micromorphology, composition and functional groups characteristics of the biofilm extracellular polymeric substances (EPS)	162:301	This paper presented the influence of Al(III) on biodegradability, micromorphology, composition and functional groups characteristics of the biofilm extracellular polymeric substances (EPS) during different growth phases.
28891420	1	41	theme	substances	286:295	arg1	characteristics					231:245	biodegradability, micromorphology, composition and functional groups characteristics	162:245	biodegradability, micromorphology, composition and functional groups characteristics of the biofilm extracellular polymeric substances (EPS)	162:301	This paper presented the influence of Al(III) on biodegradability, micromorphology, composition and functional groups characteristics of the biofilm extracellular polymeric substances (EPS) during different growth phases.
28891420	5	42	theme	toxicity	1059:1066	arg1	inhibition					1068:1077	the metal toxicity inhibition	1049:1077	the metal toxicity inhibition	1049:1077	Then, the analysis of EPS contents and components suggested that Al(III) could increase the protein (PN) of tightly bound EPS (TB-EPS) which alleviated the metal toxicity inhibition on the biofilm during the initial phases of biofilm growth.
28891420	5	43	theme	EPS	919:921	arg1	contents					923:930	EPS contents	919:930	EPS contents	919:930	Then, the analysis of EPS contents and components suggested that Al(III) could increase the protein (PN) of tightly bound EPS (TB-EPS) which alleviated the metal toxicity inhibition on the biofilm during the initial phases of biofilm growth.
28891420	4	44	theme	larger	861:866	arg1	colonies					868:875	larger colonies	861:875	larger colonies for mature biofilm	861:894	The micromorphological observation indicated that Al(III) led to a reduction of the filaments and promotion of the EPS secretion in growth phases of the biofilm, also Al(III) could promote microorganisms to form larger colonies for mature biofilm.
28891420	5	45	theme	EPS	1019:1021	arg1	protein					989:995	the protein	985:995	the protein (PN) of tightly bound EPS (TB-EPS) which alleviated the metal toxicity inhibition on the biofilm during the initial phases of biofilm growth	985:1136	Then, the analysis of EPS contents and components suggested that Al(III) could increase the protein (PN) of tightly bound EPS (TB-EPS) which alleviated the metal toxicity inhibition on the biofilm during the initial phases of biofilm growth.
28891420	5	45	theme	EPS	1019:1021	arg1	PN					998:999	PN	998:999	PN	998:999	Then, the analysis of EPS contents and components suggested that Al(III) could increase the protein (PN) of tightly bound EPS (TB-EPS) which alleviated the metal toxicity inhibition on the biofilm during the initial phases of biofilm growth.
28891420	3	46	theme	growth	546:551	arg1	beginning					525:533	the beginning	521:533	the beginning of biofilm growth	521:551	The results elucidated that Al(III) affected biofilm development adversely at the beginning of biofilm growth, but promoted the biofilm mass and improved the biofilm activity with the growth of the biofilm.
28891420	3	47	theme	biofilm	641:647	arg1	growth					627:632	the growth	623:632	the growth of the biofilm	623:647	The results elucidated that Al(III) affected biofilm development adversely at the beginning of biofilm growth, but promoted the biofilm mass and improved the biofilm activity with the growth of the biofilm.
28891420	7	48	theme	protein	1447:1453	arg1	groups					1437:1442	the functional groups	1422:1442	the functional groups of protein and polysaccharides	1422:1473	Finally, through the Fourier transform infrared spectroscopy, it was found that Al(III) was beneficial for the proliferation and secretion of TB-EPS functional groups, especially the functional groups of protein and polysaccharides.
28891420	7	48	theme	protein	1447:1453	arg1	groups					1403:1408	TB-EPS functional groups	1385:1408	TB-EPS functional groups	1385:1408	Finally, through the Fourier transform infrared spectroscopy, it was found that Al(III) was beneficial for the proliferation and secretion of TB-EPS functional groups, especially the functional groups of protein and polysaccharides.
28891420	7	48	theme	protein	1447:1453	arg1	polysaccharides					1459:1473	polysaccharides	1459:1473	polysaccharides	1459:1473	Finally, through the Fourier transform infrared spectroscopy, it was found that Al(III) was beneficial for the proliferation and secretion of TB-EPS functional groups, especially the functional groups of protein and polysaccharides.
28891420	7	48	theme	protein	1447:1453	arg1	protein					1447:1453	protein	1447:1453	protein	1447:1453	Finally, through the Fourier transform infrared spectroscopy, it was found that Al(III) was beneficial for the proliferation and secretion of TB-EPS functional groups, especially the functional groups of protein and polysaccharides.
28891420	5	49	theme	components	936:945	arg1	analysis					907:914	the analysis	903:914	the analysis of EPS contents and components	903:945	Then, the analysis of EPS contents and components suggested that Al(III) could increase the protein (PN) of tightly bound EPS (TB-EPS) which alleviated the metal toxicity inhibition on the biofilm during the initial phases of biofilm growth.
28891420	5	50	theme	bound	1013:1017	arg1	TB-EPS					1024:1029	TB-EPS	1024:1029	TB-EPS	1024:1029	Then, the analysis of EPS contents and components suggested that Al(III) could increase the protein (PN) of tightly bound EPS (TB-EPS) which alleviated the metal toxicity inhibition on the biofilm during the initial phases of biofilm growth.
28891420	5	50	theme	bound	1013:1017	arg1	EPS					1019:1021	tightly bound EPS	1005:1021	tightly bound EPS (TB-EPS) which alleviated the metal toxicity inhibition on the biofilm during the initial phases of biofilm growth	1005:1136	Then, the analysis of EPS contents and components suggested that Al(III) could increase the protein (PN) of tightly bound EPS (TB-EPS) which alleviated the metal toxicity inhibition on the biofilm during the initial phases of biofilm growth.
28891420	4	51	theme	micromorphological	654:671	arg1	observation					673:683	The micromorphological observation	650:683	The micromorphological observation	650:683	The micromorphological observation indicated that Al(III) led to a reduction of the filaments and promotion of the EPS secretion in growth phases of the biofilm, also Al(III) could promote microorganisms to form larger colonies for mature biofilm.
28891420	5	52	theme	growth	1131:1136	arg1	phases					1113:1118	the initial phases	1101:1118	the initial phases of biofilm growth	1101:1136	Then, the analysis of EPS contents and components suggested that Al(III) could increase the protein (PN) of tightly bound EPS (TB-EPS) which alleviated the metal toxicity inhibition on the biofilm during the initial phases of biofilm growth.
28891420	3	53	theme	biofilm	571:577	arg1	mass					579:582	the biofilm mass	567:582	the biofilm mass	567:582	The results elucidated that Al(III) affected biofilm development adversely at the beginning of biofilm growth, but promoted the biofilm mass and improved the biofilm activity with the growth of the biofilm.
28891420	4	54	from	promotion	747:755	arg1	phases					788:793	growth phases	781:793	growth phases of the biofilm	781:808	The micromorphological observation indicated that Al(III) led to a reduction of the filaments and promotion of the EPS secretion in growth phases of the biofilm, also Al(III) could promote microorganisms to form larger colonies for mature biofilm.
28891420	7	55	dep	Fourier	1264:1270	arg1	transform					1272:1280	transform	1272:1280	transform infrared spectroscopy	1272:1302	Finally, through the Fourier transform infrared spectroscopy, it was found that Al(III) was beneficial for the proliferation and secretion of TB-EPS functional groups, especially the functional groups of protein and polysaccharides.
28891420	1	56	theme	micromorphology	180:194	arg1	characteristics					231:245	biodegradability, micromorphology, composition and functional groups characteristics	162:245	biodegradability, micromorphology, composition and functional groups characteristics of the biofilm extracellular polymeric substances (EPS)	162:301	This paper presented the influence of Al(III) on biodegradability, micromorphology, composition and functional groups characteristics of the biofilm extracellular polymeric substances (EPS) during different growth phases.
28891420	7	57	theme	polysaccharides	1459:1473	arg1	groups					1437:1442	the functional groups	1422:1442	the functional groups of protein and polysaccharides	1422:1473	Finally, through the Fourier transform infrared spectroscopy, it was found that Al(III) was beneficial for the proliferation and secretion of TB-EPS functional groups, especially the functional groups of protein and polysaccharides.
28891420	7	57	theme	polysaccharides	1459:1473	arg1	groups					1403:1408	TB-EPS functional groups	1385:1408	TB-EPS functional groups	1385:1408	Finally, through the Fourier transform infrared spectroscopy, it was found that Al(III) was beneficial for the proliferation and secretion of TB-EPS functional groups, especially the functional groups of protein and polysaccharides.
28891420	7	57	theme	polysaccharides	1459:1473	arg1	polysaccharides					1459:1473	polysaccharides	1459:1473	polysaccharides	1459:1473	Finally, through the Fourier transform infrared spectroscopy, it was found that Al(III) was beneficial for the proliferation and secretion of TB-EPS functional groups, especially the functional groups of protein and polysaccharides.
28891420	7	57	theme	polysaccharides	1459:1473	arg1	protein					1447:1453	protein	1447:1453	protein	1447:1453	Finally, through the Fourier transform infrared spectroscopy, it was found that Al(III) was beneficial for the proliferation and secretion of TB-EPS functional groups, especially the functional groups of protein and polysaccharides.
28891420	7	58	dep	transform	1272:1280	arg1	infrared					1282:1289	infrared	1282:1289	transform infrared spectroscopy	1272:1302	Finally, through the Fourier transform infrared spectroscopy, it was found that Al(III) was beneficial for the proliferation and secretion of TB-EPS functional groups, especially the functional groups of protein and polysaccharides.
28891420	7	59	theme	functional	1392:1401	arg1	groups					1437:1442	the functional groups	1422:1442	the functional groups of protein and polysaccharides	1422:1473	Finally, through the Fourier transform infrared spectroscopy, it was found that Al(III) was beneficial for the proliferation and secretion of TB-EPS functional groups, especially the functional groups of protein and polysaccharides.
28891420	7	59	theme	functional	1392:1401	arg1	groups					1403:1408	TB-EPS functional groups	1385:1408	TB-EPS functional groups	1385:1408	Finally, through the Fourier transform infrared spectroscopy, it was found that Al(III) was beneficial for the proliferation and secretion of TB-EPS functional groups, especially the functional groups of protein and polysaccharides.
28891420	7	59	theme	functional	1392:1401	arg1	polysaccharides					1459:1473	polysaccharides	1459:1473	polysaccharides	1459:1473	Finally, through the Fourier transform infrared spectroscopy, it was found that Al(III) was beneficial for the proliferation and secretion of TB-EPS functional groups, especially the functional groups of protein and polysaccharides.
28891420	7	59	theme	functional	1392:1401	arg1	protein					1447:1453	protein	1447:1453	protein	1447:1453	Finally, through the Fourier transform infrared spectroscopy, it was found that Al(III) was beneficial for the proliferation and secretion of TB-EPS functional groups, especially the functional groups of protein and polysaccharides.
28891420	4	60	theme	mature	881:886	arg1	biofilm					888:894	mature biofilm	881:894	mature biofilm	881:894	The micromorphological observation indicated that Al(III) led to a reduction of the filaments and promotion of the EPS secretion in growth phases of the biofilm, also Al(III) could promote microorganisms to form larger colonies for mature biofilm.
28891420	0	61	from	substances	64:73	arg1	reactors					103:110	sequencing batch biofilm reactors	78:110	sequencing batch biofilm reactors	78:110	Influence of Al(III) on biofilm and its extracellular polymeric substances in sequencing batch biofilm reactors.
28891420	2	62	theme	batch	350:354	arg1	reactors					364:371	The sequencing batch biofilm reactors	335:371	The sequencing batch biofilm reactors	335:371	The sequencing batch biofilm reactors were developed to cultivate biofilms under different Al(III) dosages.
28891420	1	63	theme	composition	197:207	arg1	characteristics					231:245	biodegradability, micromorphology, composition and functional groups characteristics	162:245	biodegradability, micromorphology, composition and functional groups characteristics of the biofilm extracellular polymeric substances (EPS)	162:301	This paper presented the influence of Al(III) on biodegradability, micromorphology, composition and functional groups characteristics of the biofilm extracellular polymeric substances (EPS) during different growth phases.
28891420	6	64	theme	biofilm	1216:1222	arg1	moment					1235:1240	the biofilm maturation moment	1212:1240	the biofilm maturation moment	1212:1240	The biofilm could gradually adapt to the inhibition caused by Al(III) at the biofilm maturation moment.
28891420	2	65	theme	different	416:424	arg1	dosages					434:440	different Al(III) dosages	416:440	different Al(III) dosages	416:440	The sequencing batch biofilm reactors were developed to cultivate biofilms under different Al(III) dosages.
28891420	2	66	theme	sequencing	339:348	arg1	reactors					364:371	The sequencing batch biofilm reactors	335:371	The sequencing batch biofilm reactors	335:371	The sequencing batch biofilm reactors were developed to cultivate biofilms under different Al(III) dosages.
28891420	5	67	theme	initial	1105:1111	arg1	phases					1113:1118	the initial phases	1101:1118	the initial phases of biofilm growth	1101:1136	Then, the analysis of EPS contents and components suggested that Al(III) could increase the protein (PN) of tightly bound EPS (TB-EPS) which alleviated the metal toxicity inhibition on the biofilm during the initial phases of biofilm growth.
28891420	7	68	theme	groups	1403:1408	arg1	secretion					1372:1380	secretion	1372:1380	secretion	1372:1380	Finally, through the Fourier transform infrared spectroscopy, it was found that Al(III) was beneficial for the proliferation and secretion of TB-EPS functional groups, especially the functional groups of protein and polysaccharides.
28891420	7	68	theme	groups	1403:1408	arg1	proliferation					1354:1366	proliferation	1354:1366	proliferation	1354:1366	Finally, through the Fourier transform infrared spectroscopy, it was found that Al(III) was beneficial for the proliferation and secretion of TB-EPS functional groups, especially the functional groups of protein and polysaccharides.
27294905	2	0	theme	structural	489:498	arg1	evaluations					519:529	Compositional, structural, and morphological evaluations	474:529	Compositional, structural, and morphological evaluations	474:529	Compositional, structural, and morphological evaluations were performed by using the thermogravimetric analysis (TGA), scanning electron microscopy (SEM), and fourier transform infrared spectroscopy (FTIR) analytical techniques.
27294905	4	1	theme	count	979:983	arg1	assay					985:989	the viable cell count assay	963:989	the viable cell count assay on strains belonging to two clinically relevant bacterial species (i.e., Escherichia coli and Staphylococcus aureus)	963:1106	The evaluation of antimicrobial potential was evaluated by the viable cell count assay on strains belonging to two clinically relevant bacterial species (i.e., Escherichia coli and Staphylococcus aureus).
27294905	4	2	theme	bacterial	1039:1047	arg1	species					1049:1055	two clinically relevant bacterial species	1015:1055	two clinically relevant bacterial species (i.e., Escherichia coli and Staphylococcus aureus)	1015:1106	The evaluation of antimicrobial potential was evaluated by the viable cell count assay on strains belonging to two clinically relevant bacterial species (i.e., Escherichia coli and Staphylococcus aureus).
27294905	0	3	theme	Natural	76:82	arg1	Polymers					84:91	Natural Polymers	76:91	Natural Polymers	76:91	Fabrication, Characterization, and Evaluation of Bionanocomposites Based on Natural Polymers and Antibiotics for Wound Healing Applications.
27294905	2	4	theme	electron	602:609	arg1	SEM					623:625	SEM	623:625	SEM	623:625	Compositional, structural, and morphological evaluations were performed by using the thermogravimetric analysis (TGA), scanning electron microscopy (SEM), and fourier transform infrared spectroscopy (FTIR) analytical techniques.
27294905	2	4	theme	electron	602:609	arg1	microscopy					611:620	scanning electron microscopy	593:620	scanning electron microscopy (SEM)	593:626	Compositional, structural, and morphological evaluations were performed by using the thermogravimetric analysis (TGA), scanning electron microscopy (SEM), and fourier transform infrared spectroscopy (FTIR) analytical techniques.
27294905	1	5	theme	local	433:437	arg1	delivery					439:446	the local delivery	429:446	the local delivery of the loaded antibiotic	429:471	The aim of our research activity was to obtain a biocompatible nanostructured composite based on naturally derived biopolymers (chitin and sodium alginate) loaded with commercial antibiotics (either Cefuroxime or Cefepime) with dual functions, namely promoting wound healing and assuring the local delivery of the loaded antibiotic.
27294905	1	6	theme	research	156:163	arg1	activity					165:172	our research activity	152:172	our research activity	152:172	The aim of our research activity was to obtain a biocompatible nanostructured composite based on naturally derived biopolymers (chitin and sodium alginate) loaded with commercial antibiotics (either Cefuroxime or Cefepime) with dual functions, namely promoting wound healing and assuring the local delivery of the loaded antibiotic.
27294905	1	7	theme	antibiotic	462:471	arg1	delivery					439:446	the local delivery	429:446	the local delivery of the loaded antibiotic	429:471	The aim of our research activity was to obtain a biocompatible nanostructured composite based on naturally derived biopolymers (chitin and sodium alginate) loaded with commercial antibiotics (either Cefuroxime or Cefepime) with dual functions, namely promoting wound healing and assuring the local delivery of the loaded antibiotic.
27294905	2	8	theme	scanning	593:600	arg1	SEM					623:625	SEM	623:625	SEM	623:625	Compositional, structural, and morphological evaluations were performed by using the thermogravimetric analysis (TGA), scanning electron microscopy (SEM), and fourier transform infrared spectroscopy (FTIR) analytical techniques.
27294905	2	8	theme	scanning	593:600	arg1	microscopy					611:620	scanning electron microscopy	593:620	scanning electron microscopy (SEM)	593:626	Compositional, structural, and morphological evaluations were performed by using the thermogravimetric analysis (TGA), scanning electron microscopy (SEM), and fourier transform infrared spectroscopy (FTIR) analytical techniques.
27294905	4	9	theme	potential	936:944	arg1	evaluation					908:917	The evaluation	904:917	The evaluation of antimicrobial potential	904:944	The evaluation of antimicrobial potential was evaluated by the viable cell count assay on strains belonging to two clinically relevant bacterial species (i.e., Escherichia coli and Staphylococcus aureus).
27294905	1	10	dep	antibiotics	320:330	arg1	Cefepime					354:361	Cefepime	354:361	Cefepime	354:361	The aim of our research activity was to obtain a biocompatible nanostructured composite based on naturally derived biopolymers (chitin and sodium alginate) loaded with commercial antibiotics (either Cefuroxime or Cefepime) with dual functions, namely promoting wound healing and assuring the local delivery of the loaded antibiotic.
27294905	1	10	dep	antibiotics	320:330	arg1	antibiotics					320:330	commercial antibiotics	309:330	commercial antibiotics (either Cefuroxime or Cefepime)	309:362	The aim of our research activity was to obtain a biocompatible nanostructured composite based on naturally derived biopolymers (chitin and sodium alginate) loaded with commercial antibiotics (either Cefuroxime or Cefepime) with dual functions, namely promoting wound healing and assuring the local delivery of the loaded antibiotic.
27294905	1	10	dep	antibiotics	320:330	arg1	Cefuroxime					340:349	Cefuroxime	340:349	Cefuroxime	340:349	The aim of our research activity was to obtain a biocompatible nanostructured composite based on naturally derived biopolymers (chitin and sodium alginate) loaded with commercial antibiotics (either Cefuroxime or Cefepime) with dual functions, namely promoting wound healing and assuring the local delivery of the loaded antibiotic.
27294905	4	11	dep	species	1049:1055	arg1	i.e.					1058:1061	i.e.	1058:1061	i.e.	1058:1061	The evaluation of antimicrobial potential was evaluated by the viable cell count assay on strains belonging to two clinically relevant bacterial species (i.e., Escherichia coli and Staphylococcus aureus).
27294905	4	11	dep	species	1049:1055	arg1	coli					1076:1079	Escherichia coli	1064:1079	Escherichia coli	1064:1079	The evaluation of antimicrobial potential was evaluated by the viable cell count assay on strains belonging to two clinically relevant bacterial species (i.e., Escherichia coli and Staphylococcus aureus).
27294905	4	11	dep	species	1049:1055	arg1	aureus					1100:1105	Staphylococcus aureus	1085:1105	Staphylococcus aureus	1085:1105	The evaluation of antimicrobial potential was evaluated by the viable cell count assay on strains belonging to two clinically relevant bacterial species (i.e., Escherichia coli and Staphylococcus aureus).
27294905	0	12	theme	Wound	113:117	arg1	Healing					119:125	Wound Healing	113:125	Wound Healing Applications	113:138	Fabrication, Characterization, and Evaluation of Bionanocomposites Based on Natural Polymers and Antibiotics for Wound Healing Applications.
27294905	3	13	dep	in	865:866	arg1	vitro					868:872	vitro	868:872	vitro	868:872	In order to quantitatively and qualitatively evaluate the biocompatibility of the obtained composites, we performed the tetrazolium-salt (MTT) and agar diffusion in vitro assays on the L929 cell line.
27294905	1	14	theme	activity	165:172	arg1	aim					145:147	The aim	141:147	The aim of our research activity	141:172	The aim of our research activity was to obtain a biocompatible nanostructured composite based on naturally derived biopolymers (chitin and sodium alginate) loaded with commercial antibiotics (either Cefuroxime or Cefepime) with dual functions, namely promoting wound healing and assuring the local delivery of the loaded antibiotic.
27294905	2	15	theme	morphological	505:517	arg1	evaluations					519:529	Compositional, structural, and morphological evaluations	474:529	Compositional, structural, and morphological evaluations	474:529	Compositional, structural, and morphological evaluations were performed by using the thermogravimetric analysis (TGA), scanning electron microscopy (SEM), and fourier transform infrared spectroscopy (FTIR) analytical techniques.
27294905	1	16	theme	dual	369:372	arg1	functions					374:382	dual functions	369:382	dual functions	369:382	The aim of our research activity was to obtain a biocompatible nanostructured composite based on naturally derived biopolymers (chitin and sodium alginate) loaded with commercial antibiotics (either Cefuroxime or Cefepime) with dual functions, namely promoting wound healing and assuring the local delivery of the loaded antibiotic.
27294905	3	17	theme	L929	888:891	arg1	line					898:901	the L929 cell line	884:901	the L929 cell line	884:901	In order to quantitatively and qualitatively evaluate the biocompatibility of the obtained composites, we performed the tetrazolium-salt (MTT) and agar diffusion in vitro assays on the L929 cell line.
27294905	4	18	theme	cell	974:977	arg1	count					979:983	the viable cell count	963:983	the viable cell count assay on strains belonging to two clinically relevant bacterial species (i.e., Escherichia coli and Staphylococcus aureus)	963:1106	The evaluation of antimicrobial potential was evaluated by the viable cell count assay on strains belonging to two clinically relevant bacterial species (i.e., Escherichia coli and Staphylococcus aureus).
27294905	1	19	link	derived	248:254	arg1	chitin					269:274	chitin	269:274	chitin	269:274	The aim of our research activity was to obtain a biocompatible nanostructured composite based on naturally derived biopolymers (chitin and sodium alginate) loaded with commercial antibiotics (either Cefuroxime or Cefepime) with dual functions, namely promoting wound healing and assuring the local delivery of the loaded antibiotic.
27294905	1	19	link	derived	248:254	arg1	alginate					287:294	sodium alginate	280:294	sodium alginate	280:294	The aim of our research activity was to obtain a biocompatible nanostructured composite based on naturally derived biopolymers (chitin and sodium alginate) loaded with commercial antibiotics (either Cefuroxime or Cefepime) with dual functions, namely promoting wound healing and assuring the local delivery of the loaded antibiotic.
27294905	1	19	link	derived	248:254	arg1	biopolymers					256:266	naturally derived biopolymers	238:266	naturally derived biopolymers (chitin and sodium alginate) loaded with commercial antibiotics (either Cefuroxime or Cefepime) with dual functions, namely promoting wound healing and assuring the local delivery of the loaded antibiotic	238:471	The aim of our research activity was to obtain a biocompatible nanostructured composite based on naturally derived biopolymers (chitin and sodium alginate) loaded with commercial antibiotics (either Cefuroxime or Cefepime) with dual functions, namely promoting wound healing and assuring the local delivery of the loaded antibiotic.
27294905	3	20	theme	cell	893:896	arg1	line					898:901	the L929 cell line	884:901	the L929 cell line	884:901	In order to quantitatively and qualitatively evaluate the biocompatibility of the obtained composites, we performed the tetrazolium-salt (MTT) and agar diffusion in vitro assays on the L929 cell line.
27294905	1	21	theme	sodium	280:285	arg1	alginate					287:294	sodium alginate	280:294	sodium alginate	280:294	The aim of our research activity was to obtain a biocompatible nanostructured composite based on naturally derived biopolymers (chitin and sodium alginate) loaded with commercial antibiotics (either Cefuroxime or Cefepime) with dual functions, namely promoting wound healing and assuring the local delivery of the loaded antibiotic.
27294905	1	21	theme	sodium	280:285	arg1	biopolymers					256:266	naturally derived biopolymers	238:266	naturally derived biopolymers (chitin and sodium alginate) loaded with commercial antibiotics (either Cefuroxime or Cefepime) with dual functions, namely promoting wound healing and assuring the local delivery of the loaded antibiotic	238:471	The aim of our research activity was to obtain a biocompatible nanostructured composite based on naturally derived biopolymers (chitin and sodium alginate) loaded with commercial antibiotics (either Cefuroxime or Cefepime) with dual functions, namely promoting wound healing and assuring the local delivery of the loaded antibiotic.
27294905	3	22	theme	composites	794:803	arg1	biocompatibility					761:776	the biocompatibility	757:776	the biocompatibility of the obtained composites	757:803	In order to quantitatively and qualitatively evaluate the biocompatibility of the obtained composites, we performed the tetrazolium-salt (MTT) and agar diffusion in vitro assays on the L929 cell line.
27294905	4	23	theme	relevant	1030:1037	arg1	species					1049:1055	two clinically relevant bacterial species	1015:1055	two clinically relevant bacterial species (i.e., Escherichia coli and Staphylococcus aureus)	1015:1106	The evaluation of antimicrobial potential was evaluated by the viable cell count assay on strains belonging to two clinically relevant bacterial species (i.e., Escherichia coli and Staphylococcus aureus).
27294905	2	24	theme	thermogravimetric	559:575	arg1	TGA					587:589	TGA	587:589	TGA	587:589	Compositional, structural, and morphological evaluations were performed by using the thermogravimetric analysis (TGA), scanning electron microscopy (SEM), and fourier transform infrared spectroscopy (FTIR) analytical techniques.
27294905	2	24	theme	thermogravimetric	559:575	arg1	analysis					577:584	the thermogravimetric analysis	555:584	the thermogravimetric analysis (TGA)	555:590	Compositional, structural, and morphological evaluations were performed by using the thermogravimetric analysis (TGA), scanning electron microscopy (SEM), and fourier transform infrared spectroscopy (FTIR) analytical techniques.
27294905	2	25	theme	infrared	651:658	arg1	FTIR					674:677	FTIR	674:677	FTIR	674:677	Compositional, structural, and morphological evaluations were performed by using the thermogravimetric analysis (TGA), scanning electron microscopy (SEM), and fourier transform infrared spectroscopy (FTIR) analytical techniques.
27294905	2	25	theme	infrared	651:658	arg1	spectroscopy					660:671	infrared spectroscopy	651:671	infrared spectroscopy (FTIR)	651:678	Compositional, structural, and morphological evaluations were performed by using the thermogravimetric analysis (TGA), scanning electron microscopy (SEM), and fourier transform infrared spectroscopy (FTIR) analytical techniques.
27294905	0	26	theme	Healing	119:125	arg1	Applications					127:138	Wound Healing Applications	113:138	Wound Healing Applications	113:138	Fabrication, Characterization, and Evaluation of Bionanocomposites Based on Natural Polymers and Antibiotics for Wound Healing Applications.
27294905	1	27	theme	biocompatible	190:202	arg1	composite					219:227	a biocompatible nanostructured composite	188:227	a biocompatible nanostructured composite based on naturally derived biopolymers (chitin and sodium alginate) loaded with commercial antibiotics (either Cefuroxime or Cefepime) with dual functions, namely promoting wound healing and assuring the local delivery of the loaded antibiotic	188:471	The aim of our research activity was to obtain a biocompatible nanostructured composite based on naturally derived biopolymers (chitin and sodium alginate) loaded with commercial antibiotics (either Cefuroxime or Cefepime) with dual functions, namely promoting wound healing and assuring the local delivery of the loaded antibiotic.
27294905	2	28	theme	Compositional	474:486	arg1	evaluations					519:529	Compositional, structural, and morphological evaluations	474:529	Compositional, structural, and morphological evaluations	474:529	Compositional, structural, and morphological evaluations were performed by using the thermogravimetric analysis (TGA), scanning electron microscopy (SEM), and fourier transform infrared spectroscopy (FTIR) analytical techniques.
27294905	1	29	dep	Cefuroxime	340:349	arg1	either					333:338	either	333:338	either	333:338	The aim of our research activity was to obtain a biocompatible nanostructured composite based on naturally derived biopolymers (chitin and sodium alginate) loaded with commercial antibiotics (either Cefuroxime or Cefepime) with dual functions, namely promoting wound healing and assuring the local delivery of the loaded antibiotic.
27294905	4	30	theme	antimicrobial	922:934	arg1	potential					936:944	antimicrobial potential	922:944	antimicrobial potential	922:944	The evaluation of antimicrobial potential was evaluated by the viable cell count assay on strains belonging to two clinically relevant bacterial species (i.e., Escherichia coli and Staphylococcus aureus).
27294905	1	31	theme	nanostructured	204:217	arg1	composite					219:227	a biocompatible nanostructured composite	188:227	a biocompatible nanostructured composite based on naturally derived biopolymers (chitin and sodium alginate) loaded with commercial antibiotics (either Cefuroxime or Cefepime) with dual functions, namely promoting wound healing and assuring the local delivery of the loaded antibiotic	188:471	The aim of our research activity was to obtain a biocompatible nanostructured composite based on naturally derived biopolymers (chitin and sodium alginate) loaded with commercial antibiotics (either Cefuroxime or Cefepime) with dual functions, namely promoting wound healing and assuring the local delivery of the loaded antibiotic.
27294905	3	32	theme	obtained	785:792	arg1	composites					794:803	the obtained composites	781:803	the obtained composites	781:803	In order to quantitatively and qualitatively evaluate the biocompatibility of the obtained composites, we performed the tetrazolium-salt (MTT) and agar diffusion in vitro assays on the L929 cell line.
27294905	0	33	theme	Bionanocomposites	49:65	arg1	Characterization					13:28	Characterization	13:28	Characterization	13:28	Fabrication, Characterization, and Evaluation of Bionanocomposites Based on Natural Polymers and Antibiotics for Wound Healing Applications.
27294905	0	33	theme	Bionanocomposites	49:65	arg1	Evaluation					35:44	Evaluation	35:44	Evaluation	35:44	Fabrication, Characterization, and Evaluation of Bionanocomposites Based on Natural Polymers and Antibiotics for Wound Healing Applications.
27294905	0	33	theme	Bionanocomposites	49:65	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication	0:10	Fabrication, Characterization, and Evaluation of Bionanocomposites Based on Natural Polymers and Antibiotics for Wound Healing Applications.
27294905	1	34	theme	commercial	309:318	arg1	Cefepime					354:361	Cefepime	354:361	Cefepime	354:361	The aim of our research activity was to obtain a biocompatible nanostructured composite based on naturally derived biopolymers (chitin and sodium alginate) loaded with commercial antibiotics (either Cefuroxime or Cefepime) with dual functions, namely promoting wound healing and assuring the local delivery of the loaded antibiotic.
27294905	1	34	theme	commercial	309:318	arg1	antibiotics					320:330	commercial antibiotics	309:330	commercial antibiotics (either Cefuroxime or Cefepime)	309:362	The aim of our research activity was to obtain a biocompatible nanostructured composite based on naturally derived biopolymers (chitin and sodium alginate) loaded with commercial antibiotics (either Cefuroxime or Cefepime) with dual functions, namely promoting wound healing and assuring the local delivery of the loaded antibiotic.
27294905	1	34	theme	commercial	309:318	arg1	Cefuroxime					340:349	Cefuroxime	340:349	Cefuroxime	340:349	The aim of our research activity was to obtain a biocompatible nanostructured composite based on naturally derived biopolymers (chitin and sodium alginate) loaded with commercial antibiotics (either Cefuroxime or Cefepime) with dual functions, namely promoting wound healing and assuring the local delivery of the loaded antibiotic.
27294905	1	35	theme	wound	402:406	arg1	healing					408:414	wound healing	402:414	wound healing	402:414	The aim of our research activity was to obtain a biocompatible nanostructured composite based on naturally derived biopolymers (chitin and sodium alginate) loaded with commercial antibiotics (either Cefuroxime or Cefepime) with dual functions, namely promoting wound healing and assuring the local delivery of the loaded antibiotic.
27294905	3	36	dep	tetrazolium-salt	823:838	arg1	assays					874:879	in vitro assays	865:879	in vitro assays	865:879	In order to quantitatively and qualitatively evaluate the biocompatibility of the obtained composites, we performed the tetrazolium-salt (MTT) and agar diffusion in vitro assays on the L929 cell line.
27294905	4	37	from	assay	985:989	arg1	strains					994:1000	strains	994:1000	strains belonging to two clinically relevant bacterial species (i.e., Escherichia coli and Staphylococcus aureus)	994:1106	The evaluation of antimicrobial potential was evaluated by the viable cell count assay on strains belonging to two clinically relevant bacterial species (i.e., Escherichia coli and Staphylococcus aureus).
27294905	2	38	theme	analytical	680:689	arg1	techniques					691:700	analytical techniques	680:700	analytical techniques	680:700	Compositional, structural, and morphological evaluations were performed by using the thermogravimetric analysis (TGA), scanning electron microscopy (SEM), and fourier transform infrared spectroscopy (FTIR) analytical techniques.
27294905	3	39	theme	in	865:866	arg1	assays					874:879	in vitro assays	865:879	in vitro assays	865:879	In order to quantitatively and qualitatively evaluate the biocompatibility of the obtained composites, we performed the tetrazolium-salt (MTT) and agar diffusion in vitro assays on the L929 cell line.
27294905	1	40	dep	biopolymers	256:266	arg1	chitin					269:274	chitin	269:274	chitin	269:274	The aim of our research activity was to obtain a biocompatible nanostructured composite based on naturally derived biopolymers (chitin and sodium alginate) loaded with commercial antibiotics (either Cefuroxime or Cefepime) with dual functions, namely promoting wound healing and assuring the local delivery of the loaded antibiotic.
27294905	1	40	dep	biopolymers	256:266	arg1	alginate					287:294	sodium alginate	280:294	sodium alginate	280:294	The aim of our research activity was to obtain a biocompatible nanostructured composite based on naturally derived biopolymers (chitin and sodium alginate) loaded with commercial antibiotics (either Cefuroxime or Cefepime) with dual functions, namely promoting wound healing and assuring the local delivery of the loaded antibiotic.
27294905	1	40	dep	biopolymers	256:266	arg1	biopolymers					256:266	naturally derived biopolymers	238:266	naturally derived biopolymers (chitin and sodium alginate) loaded with commercial antibiotics (either Cefuroxime or Cefepime) with dual functions, namely promoting wound healing and assuring the local delivery of the loaded antibiotic	238:471	The aim of our research activity was to obtain a biocompatible nanostructured composite based on naturally derived biopolymers (chitin and sodium alginate) loaded with commercial antibiotics (either Cefuroxime or Cefepime) with dual functions, namely promoting wound healing and assuring the local delivery of the loaded antibiotic.
27294905	4	41	theme	viable	967:972	arg1	count					979:983	the viable cell count	963:983	the viable cell count assay on strains belonging to two clinically relevant bacterial species (i.e., Escherichia coli and Staphylococcus aureus)	963:1106	The evaluation of antimicrobial potential was evaluated by the viable cell count assay on strains belonging to two clinically relevant bacterial species (i.e., Escherichia coli and Staphylococcus aureus).
27294905	3	42	theme	agar	850:853	arg1	diffusion					855:863	agar diffusion	850:863	agar diffusion	850:863	In order to quantitatively and qualitatively evaluate the biocompatibility of the obtained composites, we performed the tetrazolium-salt (MTT) and agar diffusion in vitro assays on the L929 cell line.
27294905	2	43	dep	analysis	577:584	arg1	techniques					691:700	analytical techniques	680:700	analytical techniques	680:700	Compositional, structural, and morphological evaluations were performed by using the thermogravimetric analysis (TGA), scanning electron microscopy (SEM), and fourier transform infrared spectroscopy (FTIR) analytical techniques.
27294905	1	44	theme	derived	248:254	arg1	chitin					269:274	chitin	269:274	chitin	269:274	The aim of our research activity was to obtain a biocompatible nanostructured composite based on naturally derived biopolymers (chitin and sodium alginate) loaded with commercial antibiotics (either Cefuroxime or Cefepime) with dual functions, namely promoting wound healing and assuring the local delivery of the loaded antibiotic.
27294905	1	44	theme	derived	248:254	arg1	alginate					287:294	sodium alginate	280:294	sodium alginate	280:294	The aim of our research activity was to obtain a biocompatible nanostructured composite based on naturally derived biopolymers (chitin and sodium alginate) loaded with commercial antibiotics (either Cefuroxime or Cefepime) with dual functions, namely promoting wound healing and assuring the local delivery of the loaded antibiotic.
27294905	1	44	theme	derived	248:254	arg1	biopolymers					256:266	naturally derived biopolymers	238:266	naturally derived biopolymers (chitin and sodium alginate) loaded with commercial antibiotics (either Cefuroxime or Cefepime) with dual functions, namely promoting wound healing and assuring the local delivery of the loaded antibiotic	238:471	The aim of our research activity was to obtain a biocompatible nanostructured composite based on naturally derived biopolymers (chitin and sodium alginate) loaded with commercial antibiotics (either Cefuroxime or Cefepime) with dual functions, namely promoting wound healing and assuring the local delivery of the loaded antibiotic.
27294905	2	45	dep	fourier	633:639	arg1	transform					641:649	transform	641:649	transform infrared spectroscopy (FTIR)	641:678	Compositional, structural, and morphological evaluations were performed by using the thermogravimetric analysis (TGA), scanning electron microscopy (SEM), and fourier transform infrared spectroscopy (FTIR) analytical techniques.
27294905	1	46	dep	functions	374:382	arg1	promoting					392:400	promoting	392:400	promoting wound healing	392:414	The aim of our research activity was to obtain a biocompatible nanostructured composite based on naturally derived biopolymers (chitin and sodium alginate) loaded with commercial antibiotics (either Cefuroxime or Cefepime) with dual functions, namely promoting wound healing and assuring the local delivery of the loaded antibiotic.
27294905	1	46	dep	functions	374:382	arg1	assuring					420:427	assuring	420:427	assuring the local delivery of the loaded antibiotic	420:471	The aim of our research activity was to obtain a biocompatible nanostructured composite based on naturally derived biopolymers (chitin and sodium alginate) loaded with commercial antibiotics (either Cefuroxime or Cefepime) with dual functions, namely promoting wound healing and assuring the local delivery of the loaded antibiotic.
25608087	4	0	theme	Lentinula	541:549	arg1	edodes					551:556	Lentinula edodes	541:556	Lentinula edodes	541:556	In this study, high-throughput pyrosequencing technique was employed to investigate the effects of a new heteropolysaccharide L2 from Lentinula edodes on microbiota diversity and composition of small intestine, cecum, colon and distal end of colon (feces) in mice.
25608087	9	1	theme	L2-treated	1391:1400	arg1	mice					1402:1405	L2-treated mice	1391:1405	L2-treated mice	1391:1405	In particular, 4 phyla Chloroflexi, Gemmatimonadetes, Nitrospirae and Planctomycetes are exclusively present in L2-treated mice.
25608087	7	2	theme	fecal	917:921	arg1	communities					933:943	the fecal microbial communities	913:943	the fecal microbial communities	913:943	In the fecal microbial communities, the decrease of Bacteroidetes by significantly increasing Proteobacteria were observed, which were characterized by the increased Helicobacteraceae and reduced S24-7 at family level.
25608087	4	3	theme	colon	625:629	arg1	composition					586:596	composition	586:596	composition	586:596	In this study, high-throughput pyrosequencing technique was employed to investigate the effects of a new heteropolysaccharide L2 from Lentinula edodes on microbiota diversity and composition of small intestine, cecum, colon and distal end of colon (feces) in mice.
25608087	4	3	theme	colon	625:629	arg1	diversity					572:580	microbiota diversity	561:580	microbiota diversity	561:580	In this study, high-throughput pyrosequencing technique was employed to investigate the effects of a new heteropolysaccharide L2 from Lentinula edodes on microbiota diversity and composition of small intestine, cecum, colon and distal end of colon (feces) in mice.
25608087	3	4	theme	gut	366:368	arg1	microbes					370:377	the gut microbes	362:377	the gut microbes	362:377	However, the impact of Lentinula edodes-derived polysaccharides on the gut microbes have not yet been explored.
25608087	4	5	theme	microbiota	561:570	arg1	diversity					572:580	microbiota diversity	561:580	microbiota diversity	561:580	In this study, high-throughput pyrosequencing technique was employed to investigate the effects of a new heteropolysaccharide L2 from Lentinula edodes on microbiota diversity and composition of small intestine, cecum, colon and distal end of colon (feces) in mice.
25608087	4	6	theme	high-throughput	422:436	arg1	technique					453:461	high-throughput pyrosequencing technique	422:461	high-throughput pyrosequencing technique	422:461	In this study, high-throughput pyrosequencing technique was employed to investigate the effects of a new heteropolysaccharide L2 from Lentinula edodes on microbiota diversity and composition of small intestine, cecum, colon and distal end of colon (feces) in mice.
25608087	5	7	theme	different	758:766	arg1	distribution					774:785	distinctly different space distribution	747:785	distinctly different space distribution	747:785	The results demonstrated that along mouse intestine the microbiota exhibit distinctly different space distribution.
25608087	1	8	contain	possess	137:143	arg2	immuno-modulation					204:220	immuno-modulation	204:220	immuno-modulation	204:220	Lentinula edodes-derived polysaccharides possess many therapeutic characteristics, including anti-tumor and immuno-modulation.
25608087	1	8	contain	possess	137:143	arg2	anti-tumor					189:198	anti-tumor	189:198	anti-tumor	189:198	Lentinula edodes-derived polysaccharides possess many therapeutic characteristics, including anti-tumor and immuno-modulation.
25608087	1	8	contain	possess	137:143	arg2	characteristics					162:176	many therapeutic characteristics	145:176	many therapeutic characteristics	145:176	Lentinula edodes-derived polysaccharides possess many therapeutic characteristics, including anti-tumor and immuno-modulation.
25608087	1	8	contain	possess	137:143	arg1	polysaccharides					121:135	Lentinula edodes-derived polysaccharides	96:135	Lentinula edodes-derived polysaccharides	96:135	Lentinula edodes-derived polysaccharides possess many therapeutic characteristics, including anti-tumor and immuno-modulation.
25608087	4	9	from	edodes	551:556	arg1	L2					533:534	a new heteropolysaccharide L2	506:534	a new heteropolysaccharide L2 from Lentinula edodes	506:556	In this study, high-throughput pyrosequencing technique was employed to investigate the effects of a new heteropolysaccharide L2 from Lentinula edodes on microbiota diversity and composition of small intestine, cecum, colon and distal end of colon (feces) in mice.
25608087	4	9	from	edodes	551:556	arg1	effects					495:501	the effects	491:501	the effects of a new heteropolysaccharide L2 from Lentinula edodes on microbiota diversity and composition of small intestine, cecum, colon and distal end of colon (feces) in mice	491:669	In this study, high-throughput pyrosequencing technique was employed to investigate the effects of a new heteropolysaccharide L2 from Lentinula edodes on microbiota diversity and composition of small intestine, cecum, colon and distal end of colon (feces) in mice.
25608087	6	10	theme	microbiota	843:852	arg1	evenness					827:834	evenness	827:834	evenness	827:834	L2 treatment reduced the diversity and evenness of gut microbiota along the intestine, especially in the cecum and colon.
25608087	6	10	theme	microbiota	843:852	arg1	diversity					813:821	diversity	813:821	diversity	813:821	L2 treatment reduced the diversity and evenness of gut microbiota along the intestine, especially in the cecum and colon.
25608087	3	11	from	impact	308:313	arg1	microbes					370:377	the gut microbes	362:377	the gut microbes	362:377	However, the impact of Lentinula edodes-derived polysaccharides on the gut microbes have not yet been explored.
25608087	5	12	theme	space	768:772	arg1	distribution					774:785	distinctly different space distribution	747:785	distinctly different space distribution	747:785	The results demonstrated that along mouse intestine the microbiota exhibit distinctly different space distribution.
25608087	4	13	theme	heteropolysaccharide	512:531	arg1	L2					533:534	a new heteropolysaccharide L2	506:534	a new heteropolysaccharide L2 from Lentinula edodes	506:556	In this study, high-throughput pyrosequencing technique was employed to investigate the effects of a new heteropolysaccharide L2 from Lentinula edodes on microbiota diversity and composition of small intestine, cecum, colon and distal end of colon (feces) in mice.
25608087	7	14	theme	increased	1066:1074	arg1	Helicobacteraceae					1076:1092	the increased Helicobacteraceae	1062:1092	the increased Helicobacteraceae	1062:1092	In the fecal microbial communities, the decrease of Bacteroidetes by significantly increasing Proteobacteria were observed, which were characterized by the increased Helicobacteraceae and reduced S24-7 at family level.
25608087	0	15	link	edodes-derived	10:23	arg1	polysaccharide					25:38	Lentinula edodes-derived polysaccharide	0:38	Lentinula edodes-derived polysaccharide	0:38	Lentinula edodes-derived polysaccharide alters the spatial structure of gut microbiota in mice.
25608087	9	16	from	particular	1282:1291	arg1	present					1380:1386	present	1380:1386	present	1380:1386	In particular, 4 phyla Chloroflexi, Gemmatimonadetes, Nitrospirae and Planctomycetes are exclusively present in L2-treated mice.
25608087	0	17	from	structure	59:67	arg1	mice					90:93	mice	90:93	mice	90:93	Lentinula edodes-derived polysaccharide alters the spatial structure of gut microbiota in mice.
25608087	4	18	theme	pyrosequencing	438:451	arg1	technique					453:461	high-throughput pyrosequencing technique	422:461	high-throughput pyrosequencing technique	422:461	In this study, high-throughput pyrosequencing technique was employed to investigate the effects of a new heteropolysaccharide L2 from Lentinula edodes on microbiota diversity and composition of small intestine, cecum, colon and distal end of colon (feces) in mice.
25608087	6	19	dep	diversity	813:821	arg1	the					809:811	the	809:811	the	809:811	L2 treatment reduced the diversity and evenness of gut microbiota along the intestine, especially in the cecum and colon.
25608087	10	20	link	edodes-derived	1488:1501	arg1	L2					1518:1519	Lentinula edodes-derived polysaccharide L2	1478:1519	Lentinula edodes-derived polysaccharide L2	1478:1519	This is helpful for further demonstrating healthy action mechanism of Lentinula edodes-derived polysaccharide L2.
25608087	7	21	theme	Bacteroidetes	962:974	arg1	decrease					950:957	the decrease	946:957	the decrease of Bacteroidetes by significantly increasing Proteobacteria	946:1017	In the fecal microbial communities, the decrease of Bacteroidetes by significantly increasing Proteobacteria were observed, which were characterized by the increased Helicobacteraceae and reduced S24-7 at family level.
25608087	0	22	theme	edodes-derived	10:23	arg1	polysaccharide					25:38	Lentinula edodes-derived polysaccharide	0:38	Lentinula edodes-derived polysaccharide	0:38	Lentinula edodes-derived polysaccharide alters the spatial structure of gut microbiota in mice.
25608087	4	23	theme	L2	533:534	arg1	effects					495:501	the effects	491:501	the effects of a new heteropolysaccharide L2 from Lentinula edodes on microbiota diversity and composition of small intestine, cecum, colon and distal end of colon (feces) in mice	491:669	In this study, high-throughput pyrosequencing technique was employed to investigate the effects of a new heteropolysaccharide L2 from Lentinula edodes on microbiota diversity and composition of small intestine, cecum, colon and distal end of colon (feces) in mice.
25608087	9	24	from	mice	1402:1405	arg1	present					1380:1386	present	1380:1386	present	1380:1386	In particular, 4 phyla Chloroflexi, Gemmatimonadetes, Nitrospirae and Planctomycetes are exclusively present in L2-treated mice.
25608087	0	25	theme	microbiota	76:85	arg1	structure					59:67	the spatial structure	47:67	the spatial structure of gut microbiota in mice	47:93	Lentinula edodes-derived polysaccharide alters the spatial structure of gut microbiota in mice.
25608087	4	26	theme	new	508:510	arg1	L2					533:534	a new heteropolysaccharide L2	506:534	a new heteropolysaccharide L2 from Lentinula edodes	506:556	In this study, high-throughput pyrosequencing technique was employed to investigate the effects of a new heteropolysaccharide L2 from Lentinula edodes on microbiota diversity and composition of small intestine, cecum, colon and distal end of colon (feces) in mice.
25608087	0	27	theme	Lentinula	0:8	arg1	polysaccharide					25:38	Lentinula edodes-derived polysaccharide	0:38	Lentinula edodes-derived polysaccharide	0:38	Lentinula edodes-derived polysaccharide alters the spatial structure of gut microbiota in mice.
25608087	4	28	theme	intestine	607:615	arg1	composition					586:596	composition	586:596	composition	586:596	In this study, high-throughput pyrosequencing technique was employed to investigate the effects of a new heteropolysaccharide L2 from Lentinula edodes on microbiota diversity and composition of small intestine, cecum, colon and distal end of colon (feces) in mice.
25608087	4	28	theme	intestine	607:615	arg1	diversity					572:580	microbiota diversity	561:580	microbiota diversity	561:580	In this study, high-throughput pyrosequencing technique was employed to investigate the effects of a new heteropolysaccharide L2 from Lentinula edodes on microbiota diversity and composition of small intestine, cecum, colon and distal end of colon (feces) in mice.
25608087	10	29	theme	edodes-derived	1488:1501	arg1	L2					1518:1519	Lentinula edodes-derived polysaccharide L2	1478:1519	Lentinula edodes-derived polysaccharide L2	1478:1519	This is helpful for further demonstrating healthy action mechanism of Lentinula edodes-derived polysaccharide L2.
25608087	1	30	theme	Lentinula	96:104	arg1	polysaccharides					121:135	Lentinula edodes-derived polysaccharides	96:135	Lentinula edodes-derived polysaccharides	96:135	Lentinula edodes-derived polysaccharides possess many therapeutic characteristics, including anti-tumor and immuno-modulation.
25608087	9	31	attach	present	1380:1386	arg2	Nitrospirae					1333:1343	Nitrospirae	1333:1343	Nitrospirae	1333:1343	In particular, 4 phyla Chloroflexi, Gemmatimonadetes, Nitrospirae and Planctomycetes are exclusively present in L2-treated mice.
25608087	9	31	attach	present	1380:1386	arg1	particular					1282:1291	particular	1282:1291	particular	1282:1291	In particular, 4 phyla Chloroflexi, Gemmatimonadetes, Nitrospirae and Planctomycetes are exclusively present in L2-treated mice.
25608087	9	31	attach	present	1380:1386	arg2	Chloroflexi					1302:1312	4 phyla Chloroflexi	1294:1312	4 phyla Chloroflexi	1294:1312	In particular, 4 phyla Chloroflexi, Gemmatimonadetes, Nitrospirae and Planctomycetes are exclusively present in L2-treated mice.
25608087	9	31	attach	present	1380:1386	arg1	mice					1402:1405	L2-treated mice	1391:1405	L2-treated mice	1391:1405	In particular, 4 phyla Chloroflexi, Gemmatimonadetes, Nitrospirae and Planctomycetes are exclusively present in L2-treated mice.
25608087	9	31	attach	present	1380:1386	arg2	Planctomycetes					1349:1362	Planctomycetes	1349:1362	Planctomycetes	1349:1362	In particular, 4 phyla Chloroflexi, Gemmatimonadetes, Nitrospirae and Planctomycetes are exclusively present in L2-treated mice.
25608087	7	32	located	observed	1024:1031	arg1	communities					933:943	the fecal microbial communities	913:943	the fecal microbial communities	913:943	In the fecal microbial communities, the decrease of Bacteroidetes by significantly increasing Proteobacteria were observed, which were characterized by the increased Helicobacteraceae and reduced S24-7 at family level.
25608087	7	32	located	observed	1024:1031	arg2	decrease					950:957	the decrease	946:957	the decrease of Bacteroidetes by significantly increasing Proteobacteria	946:1017	In the fecal microbial communities, the decrease of Bacteroidetes by significantly increasing Proteobacteria were observed, which were characterized by the increased Helicobacteraceae and reduced S24-7 at family level.
25608087	4	33	theme	small	601:605	arg1	intestine					607:615	small intestine	601:615	small intestine	601:615	In this study, high-throughput pyrosequencing technique was employed to investigate the effects of a new heteropolysaccharide L2 from Lentinula edodes on microbiota diversity and composition of small intestine, cecum, colon and distal end of colon (feces) in mice.
25608087	6	34	theme	gut	839:841	arg1	microbiota					843:852	gut microbiota	839:852	gut microbiota	839:852	L2 treatment reduced the diversity and evenness of gut microbiota along the intestine, especially in the cecum and colon.
25608087	1	35	theme	edodes-derived	106:119	arg1	polysaccharides					121:135	Lentinula edodes-derived polysaccharides	96:135	Lentinula edodes-derived polysaccharides	96:135	Lentinula edodes-derived polysaccharides possess many therapeutic characteristics, including anti-tumor and immuno-modulation.
25608087	9	36	from	present	1380:1386	arg1	particular					1282:1291	particular	1282:1291	particular	1282:1291	In particular, 4 phyla Chloroflexi, Gemmatimonadetes, Nitrospirae and Planctomycetes are exclusively present in L2-treated mice.
25608087	9	36	from	present	1380:1386	arg1	mice					1402:1405	L2-treated mice	1391:1405	L2-treated mice	1391:1405	In particular, 4 phyla Chloroflexi, Gemmatimonadetes, Nitrospirae and Planctomycetes are exclusively present in L2-treated mice.
25608087	4	37	theme	cecum	618:622	arg1	composition					586:596	composition	586:596	composition	586:596	In this study, high-throughput pyrosequencing technique was employed to investigate the effects of a new heteropolysaccharide L2 from Lentinula edodes on microbiota diversity and composition of small intestine, cecum, colon and distal end of colon (feces) in mice.
25608087	4	37	theme	cecum	618:622	arg1	diversity					572:580	microbiota diversity	561:580	microbiota diversity	561:580	In this study, high-throughput pyrosequencing technique was employed to investigate the effects of a new heteropolysaccharide L2 from Lentinula edodes on microbiota diversity and composition of small intestine, cecum, colon and distal end of colon (feces) in mice.
25608087	2	38	theme	gut	227:229	arg1	microbes					231:238	The gut microbes	223:238	The gut microbes	223:238	The gut microbes play a critical role in modulation of immune function.
25608087	7	39	theme	increasing	993:1002	arg1	Proteobacteria					1004:1017	significantly increasing Proteobacteria	979:1017	significantly increasing Proteobacteria	979:1017	In the fecal microbial communities, the decrease of Bacteroidetes by significantly increasing Proteobacteria were observed, which were characterized by the increased Helicobacteraceae and reduced S24-7 at family level.
25608087	9	40	located	present	1380:1386	arg2	Nitrospirae					1333:1343	Nitrospirae	1333:1343	Nitrospirae	1333:1343	In particular, 4 phyla Chloroflexi, Gemmatimonadetes, Nitrospirae and Planctomycetes are exclusively present in L2-treated mice.
25608087	9	40	located	present	1380:1386	arg1	particular					1282:1291	particular	1282:1291	particular	1282:1291	In particular, 4 phyla Chloroflexi, Gemmatimonadetes, Nitrospirae and Planctomycetes are exclusively present in L2-treated mice.
25608087	9	40	located	present	1380:1386	arg2	Chloroflexi					1302:1312	4 phyla Chloroflexi	1294:1312	4 phyla Chloroflexi	1294:1312	In particular, 4 phyla Chloroflexi, Gemmatimonadetes, Nitrospirae and Planctomycetes are exclusively present in L2-treated mice.
25608087	9	40	located	present	1380:1386	arg1	mice					1402:1405	L2-treated mice	1391:1405	L2-treated mice	1391:1405	In particular, 4 phyla Chloroflexi, Gemmatimonadetes, Nitrospirae and Planctomycetes are exclusively present in L2-treated mice.
25608087	9	40	located	present	1380:1386	arg2	Planctomycetes					1349:1362	Planctomycetes	1349:1362	Planctomycetes	1349:1362	In particular, 4 phyla Chloroflexi, Gemmatimonadetes, Nitrospirae and Planctomycetes are exclusively present in L2-treated mice.
25608087	0	41	theme	spatial	51:57	arg1	structure					59:67	the spatial structure	47:67	the spatial structure of gut microbiota in mice	47:93	Lentinula edodes-derived polysaccharide alters the spatial structure of gut microbiota in mice.
25608087	3	42	link	edodes-derived	328:341	arg1	polysaccharides					343:357	Lentinula edodes-derived polysaccharides	318:357	Lentinula edodes-derived polysaccharides	318:357	However, the impact of Lentinula edodes-derived polysaccharides on the gut microbes have not yet been explored.
25608087	4	43	from	effects	495:501	arg1	composition					586:596	composition	586:596	composition	586:596	In this study, high-throughput pyrosequencing technique was employed to investigate the effects of a new heteropolysaccharide L2 from Lentinula edodes on microbiota diversity and composition of small intestine, cecum, colon and distal end of colon (feces) in mice.
25608087	4	43	from	effects	495:501	arg1	edodes					551:556	Lentinula edodes	541:556	Lentinula edodes	541:556	In this study, high-throughput pyrosequencing technique was employed to investigate the effects of a new heteropolysaccharide L2 from Lentinula edodes on microbiota diversity and composition of small intestine, cecum, colon and distal end of colon (feces) in mice.
25608087	4	43	from	effects	495:501	arg1	diversity					572:580	microbiota diversity	561:580	microbiota diversity	561:580	In this study, high-throughput pyrosequencing technique was employed to investigate the effects of a new heteropolysaccharide L2 from Lentinula edodes on microbiota diversity and composition of small intestine, cecum, colon and distal end of colon (feces) in mice.
25608087	4	44	from	composition	586:596	arg1	mice					666:669	mice	666:669	mice	666:669	In this study, high-throughput pyrosequencing technique was employed to investigate the effects of a new heteropolysaccharide L2 from Lentinula edodes on microbiota diversity and composition of small intestine, cecum, colon and distal end of colon (feces) in mice.
25608087	6	45	dep	cecum	893:897	arg1	the					889:891	the	889:891	the	889:891	L2 treatment reduced the diversity and evenness of gut microbiota along the intestine, especially in the cecum and colon.
25608087	7	46	theme	family	1115:1120	arg1	level					1122:1126	family level	1115:1126	family level	1115:1126	In the fecal microbial communities, the decrease of Bacteroidetes by significantly increasing Proteobacteria were observed, which were characterized by the increased Helicobacteraceae and reduced S24-7 at family level.
25608087	9	47	theme	4	1294:1294	arg1	phyla					1296:1300	phyla	1296:1300	phyla	1296:1300	In particular, 4 phyla Chloroflexi, Gemmatimonadetes, Nitrospirae and Planctomycetes are exclusively present in L2-treated mice.
25608087	10	48	theme	polysaccharide	1503:1516	arg1	L2					1518:1519	Lentinula edodes-derived polysaccharide L2	1478:1519	Lentinula edodes-derived polysaccharide L2	1478:1519	This is helpful for further demonstrating healthy action mechanism of Lentinula edodes-derived polysaccharide L2.
25608087	3	49	theme	polysaccharides	343:357	arg1	impact					308:313	the impact	304:313	the impact of Lentinula edodes-derived polysaccharides on the gut microbes	304:377	However, the impact of Lentinula edodes-derived polysaccharides on the gut microbes have not yet been explored.
25608087	9	50	theme	phyla	1296:1300	arg1	Chloroflexi					1302:1312	4 phyla Chloroflexi	1294:1312	4 phyla Chloroflexi	1294:1312	In particular, 4 phyla Chloroflexi, Gemmatimonadetes, Nitrospirae and Planctomycetes are exclusively present in L2-treated mice.
25608087	5	51	theme	mouse	708:712	arg1	intestine					714:722	mouse intestine	708:722	mouse intestine	708:722	The results demonstrated that along mouse intestine the microbiota exhibit distinctly different space distribution.
25608087	4	52	from	diversity	572:580	arg1	mice					666:669	mice	666:669	mice	666:669	In this study, high-throughput pyrosequencing technique was employed to investigate the effects of a new heteropolysaccharide L2 from Lentinula edodes on microbiota diversity and composition of small intestine, cecum, colon and distal end of colon (feces) in mice.
25608087	2	53	theme	critical	247:254	arg1	role					256:259	a critical role	245:259	a critical role	245:259	The gut microbes play a critical role in modulation of immune function.
25608087	8	54	theme	L2	1262:1263	arg1	treated-mice					1265:1276	L2 treated-mice	1262:1276	L2 treated-mice	1262:1276	Some OTUs, corresponding to Bacteroides acidifaciens, Alistipes and Helicobacter suncus, were found to be significantly increased in L2 treated-mice.
25608087	1	55	theme	many	145:148	arg1	immuno-modulation					204:220	immuno-modulation	204:220	immuno-modulation	204:220	Lentinula edodes-derived polysaccharides possess many therapeutic characteristics, including anti-tumor and immuno-modulation.
25608087	1	55	theme	many	145:148	arg1	characteristics					162:176	many therapeutic characteristics	145:176	many therapeutic characteristics	145:176	Lentinula edodes-derived polysaccharides possess many therapeutic characteristics, including anti-tumor and immuno-modulation.
25608087	1	55	theme	many	145:148	arg1	anti-tumor					189:198	anti-tumor	189:198	anti-tumor	189:198	Lentinula edodes-derived polysaccharides possess many therapeutic characteristics, including anti-tumor and immuno-modulation.
25608087	10	56	theme	Lentinula	1478:1486	arg1	L2					1518:1519	Lentinula edodes-derived polysaccharide L2	1478:1519	Lentinula edodes-derived polysaccharide L2	1478:1519	This is helpful for further demonstrating healthy action mechanism of Lentinula edodes-derived polysaccharide L2.
25608087	3	57	theme	Lentinula	318:326	arg1	polysaccharides					343:357	Lentinula edodes-derived polysaccharides	318:357	Lentinula edodes-derived polysaccharides	318:357	However, the impact of Lentinula edodes-derived polysaccharides on the gut microbes have not yet been explored.
25608087	10	58	theme	L2	1518:1519	arg1	mechanism					1465:1473	healthy action mechanism	1450:1473	healthy action mechanism of Lentinula edodes-derived polysaccharide L2	1450:1519	This is helpful for further demonstrating healthy action mechanism of Lentinula edodes-derived polysaccharide L2.
25608087	6	59	theme	L2	788:789	arg1	treatment					791:799	L2 treatment	788:799	L2 treatment	788:799	L2 treatment reduced the diversity and evenness of gut microbiota along the intestine, especially in the cecum and colon.
25608087	1	60	theme	therapeutic	150:160	arg1	immuno-modulation					204:220	immuno-modulation	204:220	immuno-modulation	204:220	Lentinula edodes-derived polysaccharides possess many therapeutic characteristics, including anti-tumor and immuno-modulation.
25608087	1	60	theme	therapeutic	150:160	arg1	characteristics					162:176	many therapeutic characteristics	145:176	many therapeutic characteristics	145:176	Lentinula edodes-derived polysaccharides possess many therapeutic characteristics, including anti-tumor and immuno-modulation.
25608087	1	60	theme	therapeutic	150:160	arg1	anti-tumor					189:198	anti-tumor	189:198	anti-tumor	189:198	Lentinula edodes-derived polysaccharides possess many therapeutic characteristics, including anti-tumor and immuno-modulation.
25608087	4	61	theme	of	646:647	arg1	feces					656:660	feces	656:660	feces	656:660	In this study, high-throughput pyrosequencing technique was employed to investigate the effects of a new heteropolysaccharide L2 from Lentinula edodes on microbiota diversity and composition of small intestine, cecum, colon and distal end of colon (feces) in mice.
25608087	4	61	theme	of	646:647	arg1	colon					649:653	distal end of colon	635:653	distal end of colon (feces)	635:661	In this study, high-throughput pyrosequencing technique was employed to investigate the effects of a new heteropolysaccharide L2 from Lentinula edodes on microbiota diversity and composition of small intestine, cecum, colon and distal end of colon (feces) in mice.
25608087	3	62	theme	edodes-derived	328:341	arg1	polysaccharides					343:357	Lentinula edodes-derived polysaccharides	318:357	Lentinula edodes-derived polysaccharides	318:357	However, the impact of Lentinula edodes-derived polysaccharides on the gut microbes have not yet been explored.
25608087	7	63	theme	microbial	923:931	arg1	communities					933:943	the fecal microbial communities	913:943	the fecal microbial communities	913:943	In the fecal microbial communities, the decrease of Bacteroidetes by significantly increasing Proteobacteria were observed, which were characterized by the increased Helicobacteraceae and reduced S24-7 at family level.
25608087	4	64	theme	end	642:644	arg1	feces					656:660	feces	656:660	feces	656:660	In this study, high-throughput pyrosequencing technique was employed to investigate the effects of a new heteropolysaccharide L2 from Lentinula edodes on microbiota diversity and composition of small intestine, cecum, colon and distal end of colon (feces) in mice.
25608087	4	64	theme	end	642:644	arg1	colon					649:653	distal end of colon	635:653	distal end of colon (feces)	635:661	In this study, high-throughput pyrosequencing technique was employed to investigate the effects of a new heteropolysaccharide L2 from Lentinula edodes on microbiota diversity and composition of small intestine, cecum, colon and distal end of colon (feces) in mice.
25608087	1	65	link	edodes-derived	106:119	arg1	polysaccharides					121:135	Lentinula edodes-derived polysaccharides	96:135	Lentinula edodes-derived polysaccharides	96:135	Lentinula edodes-derived polysaccharides possess many therapeutic characteristics, including anti-tumor and immuno-modulation.
25608087	2	66	theme	function	285:292	arg1	modulation					264:273	modulation	264:273	modulation of immune function	264:292	The gut microbes play a critical role in modulation of immune function.
25608087	10	67	theme	action	1458:1463	arg1	mechanism					1465:1473	healthy action mechanism	1450:1473	healthy action mechanism of Lentinula edodes-derived polysaccharide L2	1450:1519	This is helpful for further demonstrating healthy action mechanism of Lentinula edodes-derived polysaccharide L2.
25608087	10	68	theme	healthy	1450:1456	arg1	mechanism					1465:1473	healthy action mechanism	1450:1473	healthy action mechanism of Lentinula edodes-derived polysaccharide L2	1450:1519	This is helpful for further demonstrating healthy action mechanism of Lentinula edodes-derived polysaccharide L2.
25608087	0	69	theme	gut	72:74	arg1	microbiota					76:85	gut microbiota	72:85	gut microbiota	72:85	Lentinula edodes-derived polysaccharide alters the spatial structure of gut microbiota in mice.
25608087	2	70	theme	immune	278:283	arg1	function					285:292	immune function	278:292	immune function	278:292	The gut microbes play a critical role in modulation of immune function.
25608087	4	71	theme	distal	635:640	arg1	feces					656:660	feces	656:660	feces	656:660	In this study, high-throughput pyrosequencing technique was employed to investigate the effects of a new heteropolysaccharide L2 from Lentinula edodes on microbiota diversity and composition of small intestine, cecum, colon and distal end of colon (feces) in mice.
25608087	4	71	theme	distal	635:640	arg1	colon					649:653	distal end of colon	635:653	distal end of colon (feces)	635:661	In this study, high-throughput pyrosequencing technique was employed to investigate the effects of a new heteropolysaccharide L2 from Lentinula edodes on microbiota diversity and composition of small intestine, cecum, colon and distal end of colon (feces) in mice.
25608087	4	72	theme	colon	649:653	arg1	composition					586:596	composition	586:596	composition	586:596	In this study, high-throughput pyrosequencing technique was employed to investigate the effects of a new heteropolysaccharide L2 from Lentinula edodes on microbiota diversity and composition of small intestine, cecum, colon and distal end of colon (feces) in mice.
25608087	4	72	theme	colon	649:653	arg1	diversity					572:580	microbiota diversity	561:580	microbiota diversity	561:580	In this study, high-throughput pyrosequencing technique was employed to investigate the effects of a new heteropolysaccharide L2 from Lentinula edodes on microbiota diversity and composition of small intestine, cecum, colon and distal end of colon (feces) in mice.
28528104	6	0	from	galactose	1009:1017	arg1	rich					987:990	rich	987:990	rich	987:990	Cross-flow microfiltration produced the highest retention of polysaccharides and proanthocyanidins in all the wines, mainly polysaccharides rich in arabinose and galactose, yeast mannoproteins and highly polymerized phenols.
28528104	6	1	theme	polymerized	1051:1061	arg1	phenols					1063:1069	highly polymerized phenols	1044:1069	highly polymerized phenols	1044:1069	Cross-flow microfiltration produced the highest retention of polysaccharides and proanthocyanidins in all the wines, mainly polysaccharides rich in arabinose and galactose, yeast mannoproteins and highly polymerized phenols.
28528104	7	2	theme	rich	1088:1091	arg1	Polysaccharides					1072:1086	Polysaccharides	1072:1086	Polysaccharides rich in arabinose and mannoproteins	1072:1122	Polysaccharides rich in arabinose and mannoproteins were more retained on the ceramic membrane than polysaccharides rich in galactose and proanthocyanidins.
28528104	8	3	theme	Discriminant	1229:1240	arg1	analyses					1242:1249	Discriminant analyses	1229:1249	Discriminant analyses	1229:1249	Discriminant analyses allowed a clear differentiation of cross-flow microfiltrated wines from the rest of the wines.
28528104	7	4	from	rich	1088:1091	arg1	mannoproteins					1110:1122	mannoproteins	1110:1122	mannoproteins	1110:1122	Polysaccharides rich in arabinose and mannoproteins were more retained on the ceramic membrane than polysaccharides rich in galactose and proanthocyanidins.
28528104	7	4	from	rich	1088:1091	arg1	arabinose					1096:1104	arabinose	1096:1104	arabinose	1096:1104	Polysaccharides rich in arabinose and mannoproteins were more retained on the ceramic membrane than polysaccharides rich in galactose and proanthocyanidins.
28528104	0	5	theme	microfiltration	71:85	arg1	Effect					0:5	Effect	0:5	Effect of egg albumin fining, progressive clarification and cross-flow microfiltration on the polysaccharide and proanthocyanidin composition of red varietal wines.	0:163	Effect of egg albumin fining, progressive clarification and cross-flow microfiltration on the polysaccharide and proanthocyanidin composition of red varietal wines.
28528104	8	6	from	rest	1327:1330	arg1	differentiation					1267:1281	a clear differentiation	1259:1281	a clear differentiation of cross-flow microfiltrated wines from the rest of the wines	1259:1343	Discriminant analyses allowed a clear differentiation of cross-flow microfiltrated wines from the rest of the wines.
28528104	6	7	theme	polysaccharides	908:922	arg1	retention					895:903	the highest retention	883:903	the highest retention of polysaccharides and proanthocyanidins in all the wines, mainly polysaccharides rich in arabinose and galactose, yeast mannoproteins and highly polymerized phenols	883:1069	Cross-flow microfiltration produced the highest retention of polysaccharides and proanthocyanidins in all the wines, mainly polysaccharides rich in arabinose and galactose, yeast mannoproteins and highly polymerized phenols.
28528104	8	8	theme	wines	1312:1316	arg1	differentiation					1267:1281	a clear differentiation	1259:1281	a clear differentiation of cross-flow microfiltrated wines from the rest of the wines	1259:1343	Discriminant analyses allowed a clear differentiation of cross-flow microfiltrated wines from the rest of the wines.
28528104	3	9	theme	significant	527:537	arg1	decrease					539:546	a significant decrease	525:546	a significant decrease in the content of wine polysaccharides	525:585	Egg albumin fining did no produce a significant decrease in the content of wine polysaccharides.
28528104	0	10	theme	polysaccharide	94:107	arg1	composition					130:140	the polysaccharide and proanthocyanidin composition	90:140	the polysaccharide and proanthocyanidin composition of red varietal wines	90:162	Effect of egg albumin fining, progressive clarification and cross-flow microfiltration on the polysaccharide and proanthocyanidin composition of red varietal wines.
28528104	0	11	from	Effect	0:5	arg1	composition					130:140	the polysaccharide and proanthocyanidin composition	90:140	the polysaccharide and proanthocyanidin composition of red varietal wines	90:162	Effect of egg albumin fining, progressive clarification and cross-flow microfiltration on the polysaccharide and proanthocyanidin composition of red varietal wines.
28528104	1	12	from	effect	169:174	arg1	polysaccharide					263:276	polysaccharide	263:276	polysaccharide	263:276	The effect of egg albumin fining, progressive clarification and cross-flow microfiltration on the polysaccharide and proanthocyanidin composition of four red varietal wines was studied in this work.
28528104	1	12	from	effect	169:174	arg1	composition					299:309	proanthocyanidin composition	282:309	proanthocyanidin composition	282:309	The effect of egg albumin fining, progressive clarification and cross-flow microfiltration on the polysaccharide and proanthocyanidin composition of four red varietal wines was studied in this work.
28528104	6	13	from	retention	895:903	arg1	polysaccharides					971:985	polysaccharides	971:985	polysaccharides rich in arabinose and galactose	971:1017	Cross-flow microfiltration produced the highest retention of polysaccharides and proanthocyanidins in all the wines, mainly polysaccharides rich in arabinose and galactose, yeast mannoproteins and highly polymerized phenols.
28528104	6	13	from	retention	895:903	arg1	mannoproteins					1026:1038	yeast mannoproteins	1020:1038	yeast mannoproteins	1020:1038	Cross-flow microfiltration produced the highest retention of polysaccharides and proanthocyanidins in all the wines, mainly polysaccharides rich in arabinose and galactose, yeast mannoproteins and highly polymerized phenols.
28528104	6	13	from	retention	895:903	arg1	phenols					1063:1069	highly polymerized phenols	1044:1069	highly polymerized phenols	1044:1069	Cross-flow microfiltration produced the highest retention of polysaccharides and proanthocyanidins in all the wines, mainly polysaccharides rich in arabinose and galactose, yeast mannoproteins and highly polymerized phenols.
28528104	6	13	from	retention	895:903	arg1	wines					957:961	all the wines	949:961	all the wines	949:961	Cross-flow microfiltration produced the highest retention of polysaccharides and proanthocyanidins in all the wines, mainly polysaccharides rich in arabinose and galactose, yeast mannoproteins and highly polymerized phenols.
28528104	1	14	theme	proanthocyanidin	282:297	arg1	composition					299:309	proanthocyanidin composition	282:309	proanthocyanidin composition	282:309	The effect of egg albumin fining, progressive clarification and cross-flow microfiltration on the polysaccharide and proanthocyanidin composition of four red varietal wines was studied in this work.
28528104	5	15	theme	total	789:793	arg1	content					795:801	the total content	785:801	the total content of proanthocyanidins in all varietal wines	785:844	However, both treatments reduced the total content of proanthocyanidins in all varietal wines.
28528104	6	16	theme	rich	987:990	arg1	polysaccharides					971:985	polysaccharides	971:985	polysaccharides rich in arabinose and galactose	971:1017	Cross-flow microfiltration produced the highest retention of polysaccharides and proanthocyanidins in all the wines, mainly polysaccharides rich in arabinose and galactose, yeast mannoproteins and highly polymerized phenols.
28528104	3	17	from	decrease	539:546	arg1	content					555:561	the content	551:561	the content of wine polysaccharides	551:585	Egg albumin fining did no produce a significant decrease in the content of wine polysaccharides.
28528104	3	18	theme	wine	566:569	arg1	polysaccharides					571:585	wine polysaccharides	566:585	wine polysaccharides	566:585	Egg albumin fining did no produce a significant decrease in the content of wine polysaccharides.
28528104	4	19	theme	significant	623:633	arg1	reduction					635:643	a significant reduction	621:643	a significant reduction of mannoproteins, homogalacturonans and polysaccharides rich in arabinose and galactose in Graciano wines	621:749	Progressive clarification caused a significant reduction of mannoproteins, homogalacturonans and polysaccharides rich in arabinose and galactose in Graciano wines.
28528104	0	20	theme	proanthocyanidin	113:128	arg1	composition					130:140	the polysaccharide and proanthocyanidin composition	90:140	the polysaccharide and proanthocyanidin composition of red varietal wines	90:162	Effect of egg albumin fining, progressive clarification and cross-flow microfiltration on the polysaccharide and proanthocyanidin composition of red varietal wines.
28528104	2	21	theme	wines	444:448	arg1	differentiation					421:435	a possible differentiation	410:435	a possible differentiation of the wines according to treatment or grape variety	410:488	Discriminant analyses were applied to achieve a possible differentiation of the wines according to treatment or grape variety.
28528104	4	22	from	galactose	723:731	arg1	rich					701:704	rich	701:704	rich	701:704	Progressive clarification caused a significant reduction of mannoproteins, homogalacturonans and polysaccharides rich in arabinose and galactose in Graciano wines.
28528104	4	22	from	galactose	723:731	arg1	wines					745:749	Graciano wines	736:749	Graciano wines	736:749	Progressive clarification caused a significant reduction of mannoproteins, homogalacturonans and polysaccharides rich in arabinose and galactose in Graciano wines.
28528104	7	23	from	rich	1188:1191	arg1	proanthocyanidins					1210:1226	proanthocyanidins	1210:1226	proanthocyanidins	1210:1226	Polysaccharides rich in arabinose and mannoproteins were more retained on the ceramic membrane than polysaccharides rich in galactose and proanthocyanidins.
28528104	7	23	from	rich	1188:1191	arg1	galactose					1196:1204	galactose	1196:1204	galactose	1196:1204	Polysaccharides rich in arabinose and mannoproteins were more retained on the ceramic membrane than polysaccharides rich in galactose and proanthocyanidins.
28528104	6	24	theme	Cross-flow	847:856	arg1	microfiltration					858:872	Cross-flow microfiltration	847:872	Cross-flow microfiltration	847:872	Cross-flow microfiltration produced the highest retention of polysaccharides and proanthocyanidins in all the wines, mainly polysaccharides rich in arabinose and galactose, yeast mannoproteins and highly polymerized phenols.
28528104	7	25	theme	rich	1188:1191	arg1	polysaccharides					1172:1186	polysaccharides	1172:1186	polysaccharides rich in galactose and proanthocyanidins	1172:1226	Polysaccharides rich in arabinose and mannoproteins were more retained on the ceramic membrane than polysaccharides rich in galactose and proanthocyanidins.
28528104	1	26	theme	egg	179:181	arg1	fining					191:196	egg albumin fining	179:196	egg albumin fining	179:196	The effect of egg albumin fining, progressive clarification and cross-flow microfiltration on the polysaccharide and proanthocyanidin composition of four red varietal wines was studied in this work.
28528104	2	27	theme	grape	476:480	arg1	variety					482:488	grape variety	476:488	grape variety	476:488	Discriminant analyses were applied to achieve a possible differentiation of the wines according to treatment or grape variety.
28528104	4	28	theme	Progressive	588:598	arg1	clarification					600:612	Progressive clarification	588:612	Progressive clarification	588:612	Progressive clarification caused a significant reduction of mannoproteins, homogalacturonans and polysaccharides rich in arabinose and galactose in Graciano wines.
28528104	7	29	theme	ceramic	1150:1156	arg1	membrane					1158:1165	the ceramic membrane	1146:1165	the ceramic membrane than polysaccharides rich in galactose and proanthocyanidins	1146:1226	Polysaccharides rich in arabinose and mannoproteins were more retained on the ceramic membrane than polysaccharides rich in galactose and proanthocyanidins.
28528104	5	30	theme	proanthocyanidins	806:822	arg1	content					795:801	the total content	785:801	the total content of proanthocyanidins in all varietal wines	785:844	However, both treatments reduced the total content of proanthocyanidins in all varietal wines.
28528104	4	31	from	arabinose	709:717	arg1	rich					701:704	rich	701:704	rich	701:704	Progressive clarification caused a significant reduction of mannoproteins, homogalacturonans and polysaccharides rich in arabinose and galactose in Graciano wines.
28528104	4	31	from	arabinose	709:717	arg1	wines					745:749	Graciano wines	736:749	Graciano wines	736:749	Progressive clarification caused a significant reduction of mannoproteins, homogalacturonans and polysaccharides rich in arabinose and galactose in Graciano wines.
28528104	1	32	theme	albumin	183:189	arg1	fining					191:196	egg albumin fining	179:196	egg albumin fining	179:196	The effect of egg albumin fining, progressive clarification and cross-flow microfiltration on the polysaccharide and proanthocyanidin composition of four red varietal wines was studied in this work.
28528104	1	33	theme	fining	191:196	arg1	effect					169:174	The effect	165:174	The effect of egg albumin fining, progressive clarification and cross-flow microfiltration on the polysaccharide and proanthocyanidin composition of four red varietal wines	165:336	The effect of egg albumin fining, progressive clarification and cross-flow microfiltration on the polysaccharide and proanthocyanidin composition of four red varietal wines was studied in this work.
28528104	0	34	theme	albumin	14:20	arg1	fining					22:27	egg albumin fining	10:27	egg albumin fining	10:27	Effect of egg albumin fining, progressive clarification and cross-flow microfiltration on the polysaccharide and proanthocyanidin composition of red varietal wines.
28528104	7	35	from	galactose	1196:1204	arg1	rich					1188:1191	rich	1188:1191	rich	1188:1191	Polysaccharides rich in arabinose and mannoproteins were more retained on the ceramic membrane than polysaccharides rich in galactose and proanthocyanidins.
28528104	3	36	theme	polysaccharides	571:585	arg1	content					555:561	the content	551:561	the content of wine polysaccharides	551:585	Egg albumin fining did no produce a significant decrease in the content of wine polysaccharides.
28528104	4	37	from	rich	701:704	arg1	galactose					723:731	galactose	723:731	galactose	723:731	Progressive clarification caused a significant reduction of mannoproteins, homogalacturonans and polysaccharides rich in arabinose and galactose in Graciano wines.
28528104	4	37	from	rich	701:704	arg1	arabinose					709:717	arabinose	709:717	arabinose	709:717	Progressive clarification caused a significant reduction of mannoproteins, homogalacturonans and polysaccharides rich in arabinose and galactose in Graciano wines.
28528104	3	38	theme	Egg	491:493	arg1	fining					503:508	Egg albumin fining	491:508	Egg albumin fining	491:508	Egg albumin fining did no produce a significant decrease in the content of wine polysaccharides.
28528104	0	39	theme	egg	10:12	arg1	fining					22:27	egg albumin fining	10:27	egg albumin fining	10:27	Effect of egg albumin fining, progressive clarification and cross-flow microfiltration on the polysaccharide and proanthocyanidin composition of red varietal wines.
28528104	1	40	theme	red	319:321	arg1	wines					332:336	four red varietal wines	314:336	four red varietal wines	314:336	The effect of egg albumin fining, progressive clarification and cross-flow microfiltration on the polysaccharide and proanthocyanidin composition of four red varietal wines was studied in this work.
28528104	4	41	theme	mannoproteins	648:660	arg1	reduction					635:643	a significant reduction	621:643	a significant reduction of mannoproteins, homogalacturonans and polysaccharides rich in arabinose and galactose in Graciano wines	621:749	Progressive clarification caused a significant reduction of mannoproteins, homogalacturonans and polysaccharides rich in arabinose and galactose in Graciano wines.
28528104	5	42	from	content	795:801	arg1	wines					840:844	all varietal wines	827:844	all varietal wines	827:844	However, both treatments reduced the total content of proanthocyanidins in all varietal wines.
28528104	1	43	theme	progressive	199:209	arg1	clarification					211:223	progressive clarification	199:223	progressive clarification	199:223	The effect of egg albumin fining, progressive clarification and cross-flow microfiltration on the polysaccharide and proanthocyanidin composition of four red varietal wines was studied in this work.
28528104	7	44	from	mannoproteins	1110:1122	arg1	rich					1088:1091	rich	1088:1091	rich	1088:1091	Polysaccharides rich in arabinose and mannoproteins were more retained on the ceramic membrane than polysaccharides rich in galactose and proanthocyanidins.
28528104	1	45	theme	varietal	323:330	arg1	wines					332:336	four red varietal wines	314:336	four red varietal wines	314:336	The effect of egg albumin fining, progressive clarification and cross-flow microfiltration on the polysaccharide and proanthocyanidin composition of four red varietal wines was studied in this work.
28528104	0	46	theme	varietal	149:156	arg1	wines					158:162	red varietal wines	145:162	red varietal wines	145:162	Effect of egg albumin fining, progressive clarification and cross-flow microfiltration on the polysaccharide and proanthocyanidin composition of red varietal wines.
28528104	6	47	from	arabinose	995:1003	arg1	rich					987:990	rich	987:990	rich	987:990	Cross-flow microfiltration produced the highest retention of polysaccharides and proanthocyanidins in all the wines, mainly polysaccharides rich in arabinose and galactose, yeast mannoproteins and highly polymerized phenols.
28528104	7	48	from	proanthocyanidins	1210:1226	arg1	rich					1188:1191	rich	1188:1191	rich	1188:1191	Polysaccharides rich in arabinose and mannoproteins were more retained on the ceramic membrane than polysaccharides rich in galactose and proanthocyanidins.
28528104	7	49	from	arabinose	1096:1104	arg1	rich					1088:1091	rich	1088:1091	rich	1088:1091	Polysaccharides rich in arabinose and mannoproteins were more retained on the ceramic membrane than polysaccharides rich in galactose and proanthocyanidins.
28528104	1	50	theme	clarification	211:223	arg1	effect					169:174	The effect	165:174	The effect of egg albumin fining, progressive clarification and cross-flow microfiltration on the polysaccharide and proanthocyanidin composition of four red varietal wines	165:336	The effect of egg albumin fining, progressive clarification and cross-flow microfiltration on the polysaccharide and proanthocyanidin composition of four red varietal wines was studied in this work.
28528104	0	51	theme	fining	22:27	arg1	Effect					0:5	Effect	0:5	Effect of egg albumin fining, progressive clarification and cross-flow microfiltration on the polysaccharide and proanthocyanidin composition of red varietal wines.	0:163	Effect of egg albumin fining, progressive clarification and cross-flow microfiltration on the polysaccharide and proanthocyanidin composition of red varietal wines.
28528104	1	52	theme	wines	332:336	arg1	polysaccharide					263:276	polysaccharide	263:276	polysaccharide	263:276	The effect of egg albumin fining, progressive clarification and cross-flow microfiltration on the polysaccharide and proanthocyanidin composition of four red varietal wines was studied in this work.
28528104	1	52	theme	wines	332:336	arg1	composition					299:309	proanthocyanidin composition	282:309	proanthocyanidin composition	282:309	The effect of egg albumin fining, progressive clarification and cross-flow microfiltration on the polysaccharide and proanthocyanidin composition of four red varietal wines was studied in this work.
28528104	0	53	theme	red	145:147	arg1	wines					158:162	red varietal wines	145:162	red varietal wines	145:162	Effect of egg albumin fining, progressive clarification and cross-flow microfiltration on the polysaccharide and proanthocyanidin composition of red varietal wines.
28528104	4	54	theme	rich	701:704	arg1	mannoproteins					648:660	mannoproteins	648:660	mannoproteins	648:660	Progressive clarification caused a significant reduction of mannoproteins, homogalacturonans and polysaccharides rich in arabinose and galactose in Graciano wines.
28528104	0	55	theme	clarification	42:54	arg1	Effect					0:5	Effect	0:5	Effect of egg albumin fining, progressive clarification and cross-flow microfiltration on the polysaccharide and proanthocyanidin composition of red varietal wines.	0:163	Effect of egg albumin fining, progressive clarification and cross-flow microfiltration on the polysaccharide and proanthocyanidin composition of red varietal wines.
28528104	8	56	theme	microfiltrated	1297:1310	arg1	wines					1312:1316	cross-flow microfiltrated wines	1286:1316	cross-flow microfiltrated wines	1286:1316	Discriminant analyses allowed a clear differentiation of cross-flow microfiltrated wines from the rest of the wines.
28528104	8	57	theme	wines	1339:1343	arg1	rest					1327:1330	the rest	1323:1330	the rest of the wines	1323:1343	Discriminant analyses allowed a clear differentiation of cross-flow microfiltrated wines from the rest of the wines.
28528104	2	58	theme	Discriminant	364:375	arg1	analyses					377:384	Discriminant analyses	364:384	Discriminant analyses	364:384	Discriminant analyses were applied to achieve a possible differentiation of the wines according to treatment or grape variety.
28528104	0	59	theme	progressive	30:40	arg1	clarification					42:54	progressive clarification	30:54	progressive clarification	30:54	Effect of egg albumin fining, progressive clarification and cross-flow microfiltration on the polysaccharide and proanthocyanidin composition of red varietal wines.
28528104	6	60	theme	yeast	1020:1024	arg1	mannoproteins					1026:1038	yeast mannoproteins	1020:1038	yeast mannoproteins	1020:1038	Cross-flow microfiltration produced the highest retention of polysaccharides and proanthocyanidins in all the wines, mainly polysaccharides rich in arabinose and galactose, yeast mannoproteins and highly polymerized phenols.
28528104	0	61	theme	wines	158:162	arg1	composition					130:140	the polysaccharide and proanthocyanidin composition	90:140	the polysaccharide and proanthocyanidin composition of red varietal wines	90:162	Effect of egg albumin fining, progressive clarification and cross-flow microfiltration on the polysaccharide and proanthocyanidin composition of red varietal wines.
28528104	6	62	theme	proanthocyanidins	928:944	arg1	retention					895:903	the highest retention	883:903	the highest retention of polysaccharides and proanthocyanidins in all the wines, mainly polysaccharides rich in arabinose and galactose, yeast mannoproteins and highly polymerized phenols	883:1069	Cross-flow microfiltration produced the highest retention of polysaccharides and proanthocyanidins in all the wines, mainly polysaccharides rich in arabinose and galactose, yeast mannoproteins and highly polymerized phenols.
28528104	6	63	from	rich	987:990	arg1	arabinose					995:1003	arabinose	995:1003	arabinose	995:1003	Cross-flow microfiltration produced the highest retention of polysaccharides and proanthocyanidins in all the wines, mainly polysaccharides rich in arabinose and galactose, yeast mannoproteins and highly polymerized phenols.
28528104	6	63	from	rich	987:990	arg1	galactose					1009:1017	galactose	1009:1017	galactose	1009:1017	Cross-flow microfiltration produced the highest retention of polysaccharides and proanthocyanidins in all the wines, mainly polysaccharides rich in arabinose and galactose, yeast mannoproteins and highly polymerized phenols.
28528104	6	64	theme	highest	887:893	arg1	retention					895:903	the highest retention	883:903	the highest retention of polysaccharides and proanthocyanidins in all the wines, mainly polysaccharides rich in arabinose and galactose, yeast mannoproteins and highly polymerized phenols	883:1069	Cross-flow microfiltration produced the highest retention of polysaccharides and proanthocyanidins in all the wines, mainly polysaccharides rich in arabinose and galactose, yeast mannoproteins and highly polymerized phenols.
28528104	1	65	theme	cross-flow	229:238	arg1	microfiltration					240:254	cross-flow microfiltration	229:254	cross-flow microfiltration	229:254	The effect of egg albumin fining, progressive clarification and cross-flow microfiltration on the polysaccharide and proanthocyanidin composition of four red varietal wines was studied in this work.
28528104	1	66	dep	polysaccharide	263:276	arg1	the					259:261	the	259:261	the	259:261	The effect of egg albumin fining, progressive clarification and cross-flow microfiltration on the polysaccharide and proanthocyanidin composition of four red varietal wines was studied in this work.
28528104	5	67	theme	varietal	831:838	arg1	wines					840:844	all varietal wines	827:844	all varietal wines	827:844	However, both treatments reduced the total content of proanthocyanidins in all varietal wines.
28528104	3	68	theme	albumin	495:501	arg1	fining					503:508	Egg albumin fining	491:508	Egg albumin fining	491:508	Egg albumin fining did no produce a significant decrease in the content of wine polysaccharides.
28528104	1	69	theme	microfiltration	240:254	arg1	effect					169:174	The effect	165:174	The effect of egg albumin fining, progressive clarification and cross-flow microfiltration on the polysaccharide and proanthocyanidin composition of four red varietal wines	165:336	The effect of egg albumin fining, progressive clarification and cross-flow microfiltration on the polysaccharide and proanthocyanidin composition of four red varietal wines was studied in this work.
28528104	0	70	theme	cross-flow	60:69	arg1	microfiltration					71:85	cross-flow microfiltration	60:85	cross-flow microfiltration	60:85	Effect of egg albumin fining, progressive clarification and cross-flow microfiltration on the polysaccharide and proanthocyanidin composition of red varietal wines.
28528104	4	71	theme	polysaccharides	685:699	arg1	reduction					635:643	a significant reduction	621:643	a significant reduction of mannoproteins, homogalacturonans and polysaccharides rich in arabinose and galactose in Graciano wines	621:749	Progressive clarification caused a significant reduction of mannoproteins, homogalacturonans and polysaccharides rich in arabinose and galactose in Graciano wines.
28528104	2	72	theme	possible	412:419	arg1	differentiation					421:435	a possible differentiation	410:435	a possible differentiation of the wines according to treatment or grape variety	410:488	Discriminant analyses were applied to achieve a possible differentiation of the wines according to treatment or grape variety.
28528104	4	73	theme	homogalacturonans	663:679	arg1	reduction					635:643	a significant reduction	621:643	a significant reduction of mannoproteins, homogalacturonans and polysaccharides rich in arabinose and galactose in Graciano wines	621:749	Progressive clarification caused a significant reduction of mannoproteins, homogalacturonans and polysaccharides rich in arabinose and galactose in Graciano wines.
28528104	4	74	theme	Graciano	736:743	arg1	wines					745:749	Graciano wines	736:749	Graciano wines	736:749	Progressive clarification caused a significant reduction of mannoproteins, homogalacturonans and polysaccharides rich in arabinose and galactose in Graciano wines.
28528104	8	75	theme	clear	1261:1265	arg1	differentiation					1267:1281	a clear differentiation	1259:1281	a clear differentiation of cross-flow microfiltrated wines from the rest of the wines	1259:1343	Discriminant analyses allowed a clear differentiation of cross-flow microfiltrated wines from the rest of the wines.
28528104	8	76	theme	cross-flow	1286:1295	arg1	wines					1312:1316	cross-flow microfiltrated wines	1286:1316	cross-flow microfiltrated wines	1286:1316	Discriminant analyses allowed a clear differentiation of cross-flow microfiltrated wines from the rest of the wines.
27834877	9	0	from	expression	1320:1329	arg1	SCs					1371:1373	SCs	1371:1373	SCs	1371:1373	Then, the expression of oxidative stress-related proteins in SCs were measured by Western blot.
27834877	3	1	from	effect	390:395	arg1	SCs					451:453	high glucose-induced SCs	430:453	high glucose-induced SCs	430:453	Thus, the protective effect of Fuzi polysaccharides (FPS) on high glucose-induced SCs and the preliminary mechanism were investigated.
27834877	3	1	from	effect	390:395	arg1	mechanism					475:483	the preliminary mechanism	459:483	the preliminary mechanism	459:483	Thus, the protective effect of Fuzi polysaccharides (FPS) on high glucose-induced SCs and the preliminary mechanism were investigated.
27834877	3	2	theme	glucose-induced	435:449	arg1	SCs					451:453	high glucose-induced SCs	430:453	high glucose-induced SCs	430:453	Thus, the protective effect of Fuzi polysaccharides (FPS) on high glucose-induced SCs and the preliminary mechanism were investigated.
27834877	11	3	theme	kinase	1727:1732	arg1	activation					1741:1750	AMP-activated protein kinase (AMPK) activation	1705:1750	AMP-activated protein kinase (AMPK) activation	1705:1750	Moreover, FPS could also increase AMP-activated protein kinase (AMPK) activation significantly.
27834877	0	4	theme	Protective	73:82	arg1	Effects					84:90	Their Protective Effects	67:90	Their Protective Effects on Schwann Cells	67:107	Sugar Composition Analysis of Fuzi Polysaccharides by HPLC-MSn and Their Protective Effects on Schwann Cells Exposed to High Glucose.
27834877	7	5	theme	FPS	1096:1098	arg1	group					1072:1076	model group	1066:1076	model group	1066:1076	Next, high glucose-induced SC model was applied and divided into model group, treated group of FPS, normal and osmotic control group.
27834877	7	5	theme	FPS	1096:1098	arg1	group					1087:1091	treated group	1079:1091	treated group of FPS	1079:1098	Next, high glucose-induced SC model was applied and divided into model group, treated group of FPS, normal and osmotic control group.
27834877	7	5	theme	FPS	1096:1098	arg1	FPS					1096:1098	FPS	1096:1098	FPS	1096:1098	Next, high glucose-induced SC model was applied and divided into model group, treated group of FPS, normal and osmotic control group.
27834877	4	6	theme	liquid	646:651	arg1	chromatography					653:666	high performance liquid chromatography	629:666	high performance liquid chromatography coupled with electrospray ionization multi-tandem mass spectrometry (HPLC/ESI-MSn)	629:749	Firstly, the FPS were obtained and their monose composition was analyzed by the combination of pre-column derivatization and high performance liquid chromatography coupled with electrospray ionization multi-tandem mass spectrometry (HPLC/ESI-MSn).
27834877	12	7	theme	AMPK-PGC-1α	1802:1812	arg1	pathway					1814:1820	AMPK-PGC-1α pathway	1802:1820	AMPK-PGC-1α pathway	1802:1820	Hence, we preliminary deduced that AMPK-PGC-1α pathway may play an important role in the protective effect of FPS against high glucose-induced cell damage.
27834877	0	8	theme	Schwann	95:101	arg1	Cells					103:107	Schwann Cells	95:107	Schwann Cells	95:107	Sugar Composition Analysis of Fuzi Polysaccharides by HPLC-MSn and Their Protective Effects on Schwann Cells Exposed to High Glucose.
27834877	7	9	theme	treated	1079:1085	arg1	group					1072:1076	model group	1066:1076	model group	1066:1076	Next, high glucose-induced SC model was applied and divided into model group, treated group of FPS, normal and osmotic control group.
27834877	7	9	theme	treated	1079:1085	arg1	group					1087:1091	treated group	1079:1091	treated group of FPS	1079:1098	Next, high glucose-induced SC model was applied and divided into model group, treated group of FPS, normal and osmotic control group.
27834877	7	9	theme	treated	1079:1085	arg1	FPS					1096:1098	FPS	1096:1098	FPS	1096:1098	Next, high glucose-induced SC model was applied and divided into model group, treated group of FPS, normal and osmotic control group.
27834877	2	10	theme	Fuzi	250:253	arg1	extract					263:269	Fuzi aqueous extract	250:269	Fuzi aqueous extract	250:269	In a previous study, we have shown that Fuzi aqueous extract can attenuate Diabetic peripheral neuropathy (DPN) in rats and protect Schwann cells from injury.
27834877	4	11	theme	pre-column	599:608	arg1	derivatization					610:623	pre-column derivatization	599:623	pre-column derivatization	599:623	Firstly, the FPS were obtained and their monose composition was analyzed by the combination of pre-column derivatization and high performance liquid chromatography coupled with electrospray ionization multi-tandem mass spectrometry (HPLC/ESI-MSn).
27834877	11	12	theme	AMP-activated	1705:1717	arg1	AMPK					1735:1738	AMPK	1735:1738	AMPK	1735:1738	Moreover, FPS could also increase AMP-activated protein kinase (AMPK) activation significantly.
27834877	11	12	theme	AMP-activated	1705:1717	arg1	kinase					1727:1732	AMP-activated protein kinase	1705:1732	AMP-activated protein kinase (AMPK) activation	1705:1750	Moreover, FPS could also increase AMP-activated protein kinase (AMPK) activation significantly.
27834877	10	13	theme	receptor	1555:1562	arg1	gamma					1564:1568	peroxisome proliferator-activated receptor gamma	1521:1568	peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) protein level	1521:1611	Furthermore, the protein tests found that FPS markedly up-regulated superoxide dismutase (SOD), catalase (CAT) and peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) protein level, but down-regulated NADPH oxidase-1 (Nox1) protein level.
27834877	0	14	from	HPLC-MSn	54:61	arg1	Cells					103:107	Schwann Cells	95:107	Schwann Cells	95:107	Sugar Composition Analysis of Fuzi Polysaccharides by HPLC-MSn and Their Protective Effects on Schwann Cells Exposed to High Glucose.
27834877	8	15	theme	fluorescent	1290:1300	arg1	probes					1302:1307	the corresponding fluorescent probes	1272:1307	the corresponding fluorescent probes	1272:1307	After treatment for 48 h, the data showed FPS could significantly decrease the intracellular ROS and apoptosis, which were determined by the corresponding fluorescent probes.
27834877	4	16	theme	chromatography	653:666	arg1	combination					584:594	the combination	580:594	the combination of pre-column derivatization and high performance liquid chromatography coupled with electrospray ionization multi-tandem mass spectrometry (HPLC/ESI-MSn)	580:749	Firstly, the FPS were obtained and their monose composition was analyzed by the combination of pre-column derivatization and high performance liquid chromatography coupled with electrospray ionization multi-tandem mass spectrometry (HPLC/ESI-MSn).
27834877	4	17	theme	electrospray	681:692	arg1	spectrometry					723:734	electrospray ionization multi-tandem mass spectrometry	681:734	electrospray ionization multi-tandem mass spectrometry (HPLC/ESI-MSn)	681:749	Firstly, the FPS were obtained and their monose composition was analyzed by the combination of pre-column derivatization and high performance liquid chromatography coupled with electrospray ionization multi-tandem mass spectrometry (HPLC/ESI-MSn).
27834877	4	17	theme	electrospray	681:692	arg1	HPLC/ESI-MSn					737:748	HPLC/ESI-MSn	737:748	HPLC/ESI-MSn	737:748	Firstly, the FPS were obtained and their monose composition was analyzed by the combination of pre-column derivatization and high performance liquid chromatography coupled with electrospray ionization multi-tandem mass spectrometry (HPLC/ESI-MSn).
27834877	10	18	theme	gamma	1564:1568	arg1	level					1607:1611	peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) protein level	1521:1611	peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) protein level	1521:1611	Furthermore, the protein tests found that FPS markedly up-regulated superoxide dismutase (SOD), catalase (CAT) and peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) protein level, but down-regulated NADPH oxidase-1 (Nox1) protein level.
27834877	2	19	from	neuropathy	305:314	arg1	rats					325:328	rats	325:328	rats	325:328	In a previous study, we have shown that Fuzi aqueous extract can attenuate Diabetic peripheral neuropathy (DPN) in rats and protect Schwann cells from injury.
27834877	3	20	theme	preliminary	463:473	arg1	mechanism					475:483	the preliminary mechanism	459:483	the preliminary mechanism	459:483	Thus, the protective effect of Fuzi polysaccharides (FPS) on high glucose-induced SCs and the preliminary mechanism were investigated.
27834877	10	21	theme	protein	1599:1605	arg1	level					1607:1611	peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) protein level	1521:1611	peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) protein level	1521:1611	Furthermore, the protein tests found that FPS markedly up-regulated superoxide dismutase (SOD), catalase (CAT) and peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) protein level, but down-regulated NADPH oxidase-1 (Nox1) protein level.
27834877	3	22	theme	protective	379:388	arg1	effect					390:395	the protective effect	375:395	the protective effect of Fuzi polysaccharides (FPS) on high glucose-induced SCs and the preliminary mechanism	375:483	Thus, the protective effect of Fuzi polysaccharides (FPS) on high glucose-induced SCs and the preliminary mechanism were investigated.
27834877	10	23	theme	coactivator	1570:1580	arg1	level					1607:1611	peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) protein level	1521:1611	peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) protein level	1521:1611	Furthermore, the protein tests found that FPS markedly up-regulated superoxide dismutase (SOD), catalase (CAT) and peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) protein level, but down-regulated NADPH oxidase-1 (Nox1) protein level.
27834877	10	24	theme	PGC-1α	1591:1596	arg1	level					1607:1611	peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) protein level	1521:1611	peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) protein level	1521:1611	Furthermore, the protein tests found that FPS markedly up-regulated superoxide dismutase (SOD), catalase (CAT) and peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) protein level, but down-regulated NADPH oxidase-1 (Nox1) protein level.
27834877	8	25	dep	FPS	1177:1179	arg1	decrease					1201:1208	decrease	1201:1208	could significantly decrease the intracellular ROS and apoptosis	1181:1244	After treatment for 48 h, the data showed FPS could significantly decrease the intracellular ROS and apoptosis, which were determined by the corresponding fluorescent probes.
27834877	9	26	theme	stress-related	1344:1357	arg1	proteins					1359:1366	oxidative stress-related proteins	1334:1366	oxidative stress-related proteins	1334:1366	Then, the expression of oxidative stress-related proteins in SCs were measured by Western blot.
27834877	2	27	theme	Schwann	342:348	arg1	cells					350:354	Schwann cells	342:354	Schwann cells from injury	342:366	In a previous study, we have shown that Fuzi aqueous extract can attenuate Diabetic peripheral neuropathy (DPN) in rats and protect Schwann cells from injury.
27834877	0	28	theme	Composition	6:16	arg1	Analysis					18:25	Sugar Composition Analysis	0:25	Sugar Composition Analysis of Fuzi Polysaccharides by HPLC-MSn and Their Protective Effects on Schwann Cells	0:107	Sugar Composition Analysis of Fuzi Polysaccharides by HPLC-MSn and Their Protective Effects on Schwann Cells Exposed to High Glucose.
27834877	4	29	theme	mass	718:721	arg1	spectrometry					723:734	electrospray ionization multi-tandem mass spectrometry	681:734	electrospray ionization multi-tandem mass spectrometry (HPLC/ESI-MSn)	681:749	Firstly, the FPS were obtained and their monose composition was analyzed by the combination of pre-column derivatization and high performance liquid chromatography coupled with electrospray ionization multi-tandem mass spectrometry (HPLC/ESI-MSn).
27834877	4	29	theme	mass	718:721	arg1	HPLC/ESI-MSn					737:748	HPLC/ESI-MSn	737:748	HPLC/ESI-MSn	737:748	Firstly, the FPS were obtained and their monose composition was analyzed by the combination of pre-column derivatization and high performance liquid chromatography coupled with electrospray ionization multi-tandem mass spectrometry (HPLC/ESI-MSn).
27834877	10	30	theme	proliferator-activated	1532:1553	arg1	gamma					1564:1568	peroxisome proliferator-activated receptor gamma	1521:1568	peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) protein level	1521:1611	Furthermore, the protein tests found that FPS markedly up-regulated superoxide dismutase (SOD), catalase (CAT) and peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) protein level, but down-regulated NADPH oxidase-1 (Nox1) protein level.
27834877	1	31	theme	diabetic	162:169	arg1	complications					171:183	diabetic complications	162:183	diabetic complications	162:183	Fuzi has been used to treat diabetic complications for many years in china.
27834877	0	32	theme	Sugar	0:4	arg1	Analysis					18:25	Sugar Composition Analysis	0:25	Sugar Composition Analysis of Fuzi Polysaccharides by HPLC-MSn and Their Protective Effects on Schwann Cells	0:107	Sugar Composition Analysis of Fuzi Polysaccharides by HPLC-MSn and Their Protective Effects on Schwann Cells Exposed to High Glucose.
27834877	7	33	theme	model	1066:1070	arg1	group					1072:1076	model group	1066:1076	model group	1066:1076	Next, high glucose-induced SC model was applied and divided into model group, treated group of FPS, normal and osmotic control group.
27834877	7	33	theme	model	1066:1070	arg1	group					1087:1091	treated group	1079:1091	treated group of FPS	1079:1098	Next, high glucose-induced SC model was applied and divided into model group, treated group of FPS, normal and osmotic control group.
27834877	7	33	theme	model	1066:1070	arg1	FPS					1096:1098	FPS	1096:1098	FPS	1096:1098	Next, high glucose-induced SC model was applied and divided into model group, treated group of FPS, normal and osmotic control group.
27834877	7	33	theme	model	1066:1070	arg1	group					1128:1132	normal and osmotic control group	1101:1132	normal and osmotic control group	1101:1132	Next, high glucose-induced SC model was applied and divided into model group, treated group of FPS, normal and osmotic control group.
27834877	12	34	theme	important	1834:1842	arg1	role					1844:1847	an important role	1831:1847	an important role	1831:1847	Hence, we preliminary deduced that AMPK-PGC-1α pathway may play an important role in the protective effect of FPS against high glucose-induced cell damage.
27834877	4	35	theme	multi-tandem	705:716	arg1	spectrometry					723:734	electrospray ionization multi-tandem mass spectrometry	681:734	electrospray ionization multi-tandem mass spectrometry (HPLC/ESI-MSn)	681:749	Firstly, the FPS were obtained and their monose composition was analyzed by the combination of pre-column derivatization and high performance liquid chromatography coupled with electrospray ionization multi-tandem mass spectrometry (HPLC/ESI-MSn).
27834877	4	35	theme	multi-tandem	705:716	arg1	HPLC/ESI-MSn					737:748	HPLC/ESI-MSn	737:748	HPLC/ESI-MSn	737:748	Firstly, the FPS were obtained and their monose composition was analyzed by the combination of pre-column derivatization and high performance liquid chromatography coupled with electrospray ionization multi-tandem mass spectrometry (HPLC/ESI-MSn).
27834877	4	36	theme	derivatization	610:623	arg1	combination					584:594	the combination	580:594	the combination of pre-column derivatization and high performance liquid chromatography coupled with electrospray ionization multi-tandem mass spectrometry (HPLC/ESI-MSn)	580:749	Firstly, the FPS were obtained and their monose composition was analyzed by the combination of pre-column derivatization and high performance liquid chromatography coupled with electrospray ionization multi-tandem mass spectrometry (HPLC/ESI-MSn).
27834877	5	37	theme	method	797:802	arg1	efficiency					778:787	the efficiency	774:787	the efficiency of this method	774:802	The results witnessed the efficiency of this method and seven monosaccharides were tentatively identified, among which fucose was first reported.
27834877	0	38	theme	High	120:123	arg1	Glucose					125:131	High Glucose	120:131	High Glucose	120:131	Sugar Composition Analysis of Fuzi Polysaccharides by HPLC-MSn and Their Protective Effects on Schwann Cells Exposed to High Glucose.
27834877	9	39	theme	Western	1392:1398	arg1	blot					1400:1403	Western blot	1392:1403	Western blot	1392:1403	Then, the expression of oxidative stress-related proteins in SCs were measured by Western blot.
27834877	10	40	dep	protein	1656:1662	arg1	NADPH					1633:1637	NADPH	1633:1637	NADPH	1633:1637	Furthermore, the protein tests found that FPS markedly up-regulated superoxide dismutase (SOD), catalase (CAT) and peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) protein level, but down-regulated NADPH oxidase-1 (Nox1) protein level.
27834877	10	40	dep	protein	1656:1662	arg1	Nox1					1650:1653	Nox1	1650:1653	Nox1	1650:1653	Furthermore, the protein tests found that FPS markedly up-regulated superoxide dismutase (SOD), catalase (CAT) and peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) protein level, but down-regulated NADPH oxidase-1 (Nox1) protein level.
27834877	10	40	dep	protein	1656:1662	arg1	oxidase-1					1639:1647	oxidase-1	1639:1647	oxidase-1	1639:1647	Furthermore, the protein tests found that FPS markedly up-regulated superoxide dismutase (SOD), catalase (CAT) and peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) protein level, but down-regulated NADPH oxidase-1 (Nox1) protein level.
27834877	3	41	theme	polysaccharides	405:419	arg1	effect					390:395	the protective effect	375:395	the protective effect of Fuzi polysaccharides (FPS) on high glucose-induced SCs and the preliminary mechanism	375:483	Thus, the protective effect of Fuzi polysaccharides (FPS) on high glucose-induced SCs and the preliminary mechanism were investigated.
27834877	10	42	theme	peroxisome	1521:1530	arg1	gamma					1564:1568	peroxisome proliferator-activated receptor gamma	1521:1568	peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) protein level	1521:1611	Furthermore, the protein tests found that FPS markedly up-regulated superoxide dismutase (SOD), catalase (CAT) and peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) protein level, but down-regulated NADPH oxidase-1 (Nox1) protein level.
27834877	6	43	theme	diagnostic	943:952	arg1	ions					954:957	diagnostic ions	943:957	diagnostic ions	943:957	Simultaneously, m/z 215 can be considered as diagnostic ions to confirm the number of monosaccharides.
27834877	6	43	theme	diagnostic	943:952	arg1	m/z					914:916	m/z 215	914:920	m/z 215	914:920	Simultaneously, m/z 215 can be considered as diagnostic ions to confirm the number of monosaccharides.
27834877	0	44	from	Effects	84:90	arg1	Cells					103:107	Schwann Cells	95:107	Schwann Cells	95:107	Sugar Composition Analysis of Fuzi Polysaccharides by HPLC-MSn and Their Protective Effects on Schwann Cells Exposed to High Glucose.
27834877	2	45	theme	aqueous	255:261	arg1	extract					263:269	Fuzi aqueous extract	250:269	Fuzi aqueous extract	250:269	In a previous study, we have shown that Fuzi aqueous extract can attenuate Diabetic peripheral neuropathy (DPN) in rats and protect Schwann cells from injury.
27834877	1	46	theme	many	189:192	arg1	years					194:198	many years	189:198	many years in china	189:207	Fuzi has been used to treat diabetic complications for many years in china.
27834877	0	47	theme	Polysaccharides	35:49	arg1	Analysis					18:25	Sugar Composition Analysis	0:25	Sugar Composition Analysis of Fuzi Polysaccharides by HPLC-MSn and Their Protective Effects on Schwann Cells	0:107	Sugar Composition Analysis of Fuzi Polysaccharides by HPLC-MSn and Their Protective Effects on Schwann Cells Exposed to High Glucose.
27834877	12	48	theme	protective	1856:1865	arg1	effect					1867:1872	the protective effect	1852:1872	the protective effect of FPS against high glucose-induced cell damage	1852:1920	Hence, we preliminary deduced that AMPK-PGC-1α pathway may play an important role in the protective effect of FPS against high glucose-induced cell damage.
27834877	12	49	theme	glucose-induced	1894:1908	arg1	damage					1915:1920	high glucose-induced cell damage	1889:1920	high glucose-induced cell damage	1889:1920	Hence, we preliminary deduced that AMPK-PGC-1α pathway may play an important role in the protective effect of FPS against high glucose-induced cell damage.
27834877	0	50	theme	Fuzi	30:33	arg1	Polysaccharides					35:49	Fuzi Polysaccharides	30:49	Fuzi Polysaccharides	30:49	Sugar Composition Analysis of Fuzi Polysaccharides by HPLC-MSn and Their Protective Effects on Schwann Cells Exposed to High Glucose.
27834877	7	51	theme	osmotic	1112:1118	arg1	group					1072:1076	model group	1066:1076	model group	1066:1076	Next, high glucose-induced SC model was applied and divided into model group, treated group of FPS, normal and osmotic control group.
27834877	7	51	theme	osmotic	1112:1118	arg1	group					1128:1132	normal and osmotic control group	1101:1132	normal and osmotic control group	1101:1132	Next, high glucose-induced SC model was applied and divided into model group, treated group of FPS, normal and osmotic control group.
27834877	7	52	theme	control	1120:1126	arg1	group					1072:1076	model group	1066:1076	model group	1066:1076	Next, high glucose-induced SC model was applied and divided into model group, treated group of FPS, normal and osmotic control group.
27834877	7	52	theme	control	1120:1126	arg1	group					1128:1132	normal and osmotic control group	1101:1132	normal and osmotic control group	1101:1132	Next, high glucose-induced SC model was applied and divided into model group, treated group of FPS, normal and osmotic control group.
27834877	1	53	from	years	194:198	arg1	china					203:207	china	203:207	china	203:207	Fuzi has been used to treat diabetic complications for many years in china.
27834877	3	54	theme	Fuzi	400:403	arg1	FPS					422:424	FPS	422:424	FPS	422:424	Thus, the protective effect of Fuzi polysaccharides (FPS) on high glucose-induced SCs and the preliminary mechanism were investigated.
27834877	3	54	theme	Fuzi	400:403	arg1	polysaccharides					405:419	Fuzi polysaccharides	400:419	Fuzi polysaccharides (FPS)	400:425	Thus, the protective effect of Fuzi polysaccharides (FPS) on high glucose-induced SCs and the preliminary mechanism were investigated.
27834877	10	55	theme	1-alpha	1582:1588	arg1	level					1607:1611	peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) protein level	1521:1611	peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) protein level	1521:1611	Furthermore, the protein tests found that FPS markedly up-regulated superoxide dismutase (SOD), catalase (CAT) and peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) protein level, but down-regulated NADPH oxidase-1 (Nox1) protein level.
27834877	7	56	theme	SC	1028:1029	arg1	model					1031:1035	high glucose-induced SC model	1007:1035	high glucose-induced SC model	1007:1035	Next, high glucose-induced SC model was applied and divided into model group, treated group of FPS, normal and osmotic control group.
27834877	10	57	theme	superoxide	1474:1483	arg1	SOD					1496:1498	SOD	1496:1498	SOD	1496:1498	Furthermore, the protein tests found that FPS markedly up-regulated superoxide dismutase (SOD), catalase (CAT) and peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) protein level, but down-regulated NADPH oxidase-1 (Nox1) protein level.
27834877	10	57	theme	superoxide	1474:1483	arg1	dismutase					1485:1493	superoxide dismutase	1474:1493	superoxide dismutase (SOD)	1474:1499	Furthermore, the protein tests found that FPS markedly up-regulated superoxide dismutase (SOD), catalase (CAT) and peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) protein level, but down-regulated NADPH oxidase-1 (Nox1) protein level.
27834877	10	58	theme	protein	1656:1662	arg1	level					1664:1668	NADPH oxidase-1 (Nox1) protein level	1633:1668	NADPH oxidase-1 (Nox1) protein level	1633:1668	Furthermore, the protein tests found that FPS markedly up-regulated superoxide dismutase (SOD), catalase (CAT) and peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) protein level, but down-regulated NADPH oxidase-1 (Nox1) protein level.
27834877	7	59	theme	normal	1101:1106	arg1	group					1072:1076	model group	1066:1076	model group	1066:1076	Next, high glucose-induced SC model was applied and divided into model group, treated group of FPS, normal and osmotic control group.
27834877	7	59	theme	normal	1101:1106	arg1	group					1128:1132	normal and osmotic control group	1101:1132	normal and osmotic control group	1101:1132	Next, high glucose-induced SC model was applied and divided into model group, treated group of FPS, normal and osmotic control group.
27834877	11	60	theme	protein	1719:1725	arg1	AMPK					1735:1738	AMPK	1735:1738	AMPK	1735:1738	Moreover, FPS could also increase AMP-activated protein kinase (AMPK) activation significantly.
27834877	11	60	theme	protein	1719:1725	arg1	kinase					1727:1732	AMP-activated protein kinase	1705:1732	AMP-activated protein kinase (AMPK) activation	1705:1750	Moreover, FPS could also increase AMP-activated protein kinase (AMPK) activation significantly.
27834877	10	61	theme	protein	1423:1429	arg1	tests					1431:1435	the protein tests	1419:1435	the protein tests	1419:1435	Furthermore, the protein tests found that FPS markedly up-regulated superoxide dismutase (SOD), catalase (CAT) and peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) protein level, but down-regulated NADPH oxidase-1 (Nox1) protein level.
27834877	3	62	theme	high	430:433	arg1	SCs					451:453	high glucose-induced SCs	430:453	high glucose-induced SCs	430:453	Thus, the protective effect of Fuzi polysaccharides (FPS) on high glucose-induced SCs and the preliminary mechanism were investigated.
27834877	7	63	theme	high	1007:1010	arg1	model					1031:1035	high glucose-induced SC model	1007:1035	high glucose-induced SC model	1007:1035	Next, high glucose-induced SC model was applied and divided into model group, treated group of FPS, normal and osmotic control group.
27834877	2	64	theme	peripheral	294:303	arg1	neuropathy					305:314	Diabetic peripheral neuropathy	285:314	Diabetic peripheral neuropathy (DPN) in rats	285:328	In a previous study, we have shown that Fuzi aqueous extract can attenuate Diabetic peripheral neuropathy (DPN) in rats and protect Schwann cells from injury.
27834877	2	64	theme	peripheral	294:303	arg1	DPN					317:319	DPN	317:319	DPN	317:319	In a previous study, we have shown that Fuzi aqueous extract can attenuate Diabetic peripheral neuropathy (DPN) in rats and protect Schwann cells from injury.
27834877	9	65	theme	oxidative	1334:1342	arg1	proteins					1359:1366	oxidative stress-related proteins	1334:1366	oxidative stress-related proteins	1334:1366	Then, the expression of oxidative stress-related proteins in SCs were measured by Western blot.
27834877	4	66	theme	monose	545:550	arg1	composition					552:562	their monose composition	539:562	their monose composition	539:562	Firstly, the FPS were obtained and their monose composition was analyzed by the combination of pre-column derivatization and high performance liquid chromatography coupled with electrospray ionization multi-tandem mass spectrometry (HPLC/ESI-MSn).
27834877	7	67	theme	glucose-induced	1012:1026	arg1	model					1031:1035	high glucose-induced SC model	1007:1035	high glucose-induced SC model	1007:1035	Next, high glucose-induced SC model was applied and divided into model group, treated group of FPS, normal and osmotic control group.
27834877	1	68	used	used	148:151	arg2	Fuzi					134:137	Fuzi	134:137	Fuzi	134:137	Fuzi has been used to treat diabetic complications for many years in china.
27834877	2	69	from	injury	361:366	arg1	cells					350:354	Schwann cells	342:354	Schwann cells from injury	342:366	In a previous study, we have shown that Fuzi aqueous extract can attenuate Diabetic peripheral neuropathy (DPN) in rats and protect Schwann cells from injury.
27834877	2	70	theme	Diabetic	285:292	arg1	neuropathy					305:314	Diabetic peripheral neuropathy	285:314	Diabetic peripheral neuropathy (DPN) in rats	285:328	In a previous study, we have shown that Fuzi aqueous extract can attenuate Diabetic peripheral neuropathy (DPN) in rats and protect Schwann cells from injury.
27834877	2	70	theme	Diabetic	285:292	arg1	DPN					317:319	DPN	317:319	DPN	317:319	In a previous study, we have shown that Fuzi aqueous extract can attenuate Diabetic peripheral neuropathy (DPN) in rats and protect Schwann cells from injury.
27834877	8	71	theme	corresponding	1276:1288	arg1	probes					1302:1307	the corresponding fluorescent probes	1272:1307	the corresponding fluorescent probes	1272:1307	After treatment for 48 h, the data showed FPS could significantly decrease the intracellular ROS and apoptosis, which were determined by the corresponding fluorescent probes.
27834877	4	72	theme	ionization	694:703	arg1	spectrometry					723:734	electrospray ionization multi-tandem mass spectrometry	681:734	electrospray ionization multi-tandem mass spectrometry (HPLC/ESI-MSn)	681:749	Firstly, the FPS were obtained and their monose composition was analyzed by the combination of pre-column derivatization and high performance liquid chromatography coupled with electrospray ionization multi-tandem mass spectrometry (HPLC/ESI-MSn).
27834877	4	72	theme	ionization	694:703	arg1	HPLC/ESI-MSn					737:748	HPLC/ESI-MSn	737:748	HPLC/ESI-MSn	737:748	Firstly, the FPS were obtained and their monose composition was analyzed by the combination of pre-column derivatization and high performance liquid chromatography coupled with electrospray ionization multi-tandem mass spectrometry (HPLC/ESI-MSn).
27834877	8	73	theme	intracellular	1214:1226	arg1	ROS					1228:1230	the intracellular ROS	1210:1230	the intracellular ROS	1210:1230	After treatment for 48 h, the data showed FPS could significantly decrease the intracellular ROS and apoptosis, which were determined by the corresponding fluorescent probes.
27834877	4	74	theme	performance	634:644	arg1	chromatography					653:666	high performance liquid chromatography	629:666	high performance liquid chromatography coupled with electrospray ionization multi-tandem mass spectrometry (HPLC/ESI-MSn)	629:749	Firstly, the FPS were obtained and their monose composition was analyzed by the combination of pre-column derivatization and high performance liquid chromatography coupled with electrospray ionization multi-tandem mass spectrometry (HPLC/ESI-MSn).
27834877	9	75	theme	proteins	1359:1366	arg1	expression					1320:1329	the expression	1316:1329	the expression of oxidative stress-related proteins in SCs	1316:1373	Then, the expression of oxidative stress-related proteins in SCs were measured by Western blot.
27834877	12	76	theme	cell	1910:1913	arg1	damage					1915:1920	high glucose-induced cell damage	1889:1920	high glucose-induced cell damage	1889:1920	Hence, we preliminary deduced that AMPK-PGC-1α pathway may play an important role in the protective effect of FPS against high glucose-induced cell damage.
27834877	2	77	theme	previous	215:222	arg1	study					224:228	a previous study	213:228	a previous study	213:228	In a previous study, we have shown that Fuzi aqueous extract can attenuate Diabetic peripheral neuropathy (DPN) in rats and protect Schwann cells from injury.
27834877	12	78	theme	high	1889:1892	arg1	damage					1915:1920	high glucose-induced cell damage	1889:1920	high glucose-induced cell damage	1889:1920	Hence, we preliminary deduced that AMPK-PGC-1α pathway may play an important role in the protective effect of FPS against high glucose-induced cell damage.
27834877	4	79	theme	high	629:632	arg1	chromatography					653:666	high performance liquid chromatography	629:666	high performance liquid chromatography coupled with electrospray ionization multi-tandem mass spectrometry (HPLC/ESI-MSn)	629:749	Firstly, the FPS were obtained and their monose composition was analyzed by the combination of pre-column derivatization and high performance liquid chromatography coupled with electrospray ionization multi-tandem mass spectrometry (HPLC/ESI-MSn).
27834877	12	80	theme	FPS	1877:1879	arg1	effect					1867:1872	the protective effect	1852:1872	the protective effect of FPS against high glucose-induced cell damage	1852:1920	Hence, we preliminary deduced that AMPK-PGC-1α pathway may play an important role in the protective effect of FPS against high glucose-induced cell damage.
27834877	6	81	theme	monosaccharides	984:998	arg1	number					974:979	the number	970:979	the number of monosaccharides	970:998	Simultaneously, m/z 215 can be considered as diagnostic ions to confirm the number of monosaccharides.
27312610	0	0	theme	leaf	86:89	arg1	extract					91:97	leaf extract	86:97	leaf extract	86:97	Preparation of cellulose composites with in situ generated copper nanoparticles using leaf extract and their properties.
27312610	8	1	theme	composite	1039:1047	arg1	films					1049:1053	These biodegradable composite films	1019:1053	These biodegradable composite films	1019:1053	These biodegradable composite films can be considered for packaging and medical applications.
27312610	1	2	theme	copper	142:147	arg1	CuNPs					164:168	CuNPs	164:168	CuNPs	164:168	In the present work, copper nanoparticles (CuNPs) were in situ generated in cellulose matrix using Ocimum sanctum leaf extract as a reducing agent and aq.
27312610	1	2	theme	copper	142:147	arg1	nanoparticles					149:161	copper nanoparticles	142:161	copper nanoparticles (CuNPs)	142:169	In the present work, copper nanoparticles (CuNPs) were in situ generated in cellulose matrix using Ocimum sanctum leaf extract as a reducing agent and aq.
27312610	6	3	dep	transform	724:732	arg1	infrared					734:741	infrared	734:741	transform infrared spectroscopic, X-ray diffraction, thermogravimetric analysis and SEM techniques	724:821	The cellulose, matrix and the composite films were characterized by Fourier transform infrared spectroscopic, X-ray diffraction, thermogravimetric analysis and SEM techniques.
27312610	1	4	theme	sanctum	227:233	arg1	agent					262:266	a reducing agent	251:266	a reducing agent	251:266	In the present work, copper nanoparticles (CuNPs) were in situ generated in cellulose matrix using Ocimum sanctum leaf extract as a reducing agent and aq.
27312610	1	4	theme	sanctum	227:233	arg1	extract					240:246	Ocimum sanctum leaf extract	220:246	Ocimum sanctum leaf extract	220:246	In the present work, copper nanoparticles (CuNPs) were in situ generated in cellulose matrix using Ocimum sanctum leaf extract as a reducing agent and aq.
27312610	5	5	theme	CuSO4	631:635	arg1	solutions					637:645	CuSO4 solutions	631:645	CuSO4 solutions	631:645	CuSO4 solutions.
27312610	4	6	dep	Escherichia	545:555	arg1	coli					557:560	coli	557:560	coli	557:560	The composite films showed good antibacterial activity against Escherichia coli bacteria when the CuNPs were generated using higher concentrated aq.
27312610	4	7	theme	Escherichia	545:555	arg1	bacteria					562:569	Escherichia coli bacteria	545:569	Escherichia coli bacteria	545:569	The composite films showed good antibacterial activity against Escherichia coli bacteria when the CuNPs were generated using higher concentrated aq.
27312610	6	8	theme	SEM	808:810	arg1	techniques					812:821	spectroscopic, X-ray diffraction, thermogravimetric analysis and SEM techniques	743:821	spectroscopic, X-ray diffraction, thermogravimetric analysis and SEM techniques	743:821	The cellulose, matrix and the composite films were characterized by Fourier transform infrared spectroscopic, X-ray diffraction, thermogravimetric analysis and SEM techniques.
27312610	7	9	theme	conventional	928:939	arg1	polymers					941:948	the conventional polymers	924:948	the conventional polymers like polyethylene and polypropylene used for packaging applications	924:1016	The tensile strength of the composite films was lower than that of the matrix but still higher than the conventional polymers like polyethylene and polypropylene used for packaging applications.
27312610	7	10	theme	composite	852:860	arg1	films					862:866	the composite films	848:866	the composite films	848:866	The tensile strength of the composite films was lower than that of the matrix but still higher than the conventional polymers like polyethylene and polypropylene used for packaging applications.
27312610	1	11	theme	present	128:134	arg1	work					136:139	the present work	124:139	the present work	124:139	In the present work, copper nanoparticles (CuNPs) were in situ generated in cellulose matrix using Ocimum sanctum leaf extract as a reducing agent and aq.
27312610	6	12	theme	analysis	795:802	arg1	techniques					812:821	spectroscopic, X-ray diffraction, thermogravimetric analysis and SEM techniques	743:821	spectroscopic, X-ray diffraction, thermogravimetric analysis and SEM techniques	743:821	The cellulose, matrix and the composite films were characterized by Fourier transform infrared spectroscopic, X-ray diffraction, thermogravimetric analysis and SEM techniques.
27312610	6	13	theme	composite	678:686	arg1	films					688:692	the composite films	674:692	the composite films	674:692	The cellulose, matrix and the composite films were characterized by Fourier transform infrared spectroscopic, X-ray diffraction, thermogravimetric analysis and SEM techniques.
27312610	6	14	theme	thermogravimetric	777:793	arg1	analysis					795:802	thermogravimetric analysis	777:802	thermogravimetric analysis	777:802	The cellulose, matrix and the composite films were characterized by Fourier transform infrared spectroscopic, X-ray diffraction, thermogravimetric analysis and SEM techniques.
27312610	3	15	from	film	353:356	arg1	solution					365:372	the solution	361:372	the solution which were separated and viewed by Transmission electron microscope and Scanning electron microscope (SEM)	361:479	Some CuNPs were also formed outside the film in the solution which were separated and viewed by Transmission electron microscope and Scanning electron microscope (SEM).
27312610	4	16	theme	antibacterial	514:526	arg1	activity					528:535	good antibacterial activity	509:535	good antibacterial activity against Escherichia coli bacteria	509:569	The composite films showed good antibacterial activity against Escherichia coli bacteria when the CuNPs were generated using higher concentrated aq.
27312610	6	17	theme	diffraction	764:774	arg1	techniques					812:821	spectroscopic, X-ray diffraction, thermogravimetric analysis and SEM techniques	743:821	spectroscopic, X-ray diffraction, thermogravimetric analysis and SEM techniques	743:821	The cellulose, matrix and the composite films were characterized by Fourier transform infrared spectroscopic, X-ray diffraction, thermogravimetric analysis and SEM techniques.
27312610	7	18	theme	tensile	828:834	arg1	strength					836:843	The tensile strength	824:843	The tensile strength of the composite films	824:866	The tensile strength of the composite films was lower than that of the matrix but still higher than the conventional polymers like polyethylene and polypropylene used for packaging applications.
27312610	7	18	theme	tensile	828:834	arg1	lower					872:876	lower	872:876	lower	872:876	The tensile strength of the composite films was lower than that of the matrix but still higher than the conventional polymers like polyethylene and polypropylene used for packaging applications.
27312610	8	19	theme	biodegradable	1025:1037	arg1	films					1049:1053	These biodegradable composite films	1019:1053	These biodegradable composite films	1019:1053	These biodegradable composite films can be considered for packaging and medical applications.
27312610	0	20	theme	composites	25:34	arg1	Preparation					0:10	Preparation	0:10	Preparation of cellulose composites with in situ generated copper nanoparticles using leaf extract and their properties.	0:119	Preparation of cellulose composites with in situ generated copper nanoparticles using leaf extract and their properties.
27312610	4	21	theme	good	509:512	arg1	activity					528:535	good antibacterial activity	509:535	good antibacterial activity against Escherichia coli bacteria	509:569	The composite films showed good antibacterial activity against Escherichia coli bacteria when the CuNPs were generated using higher concentrated aq.
27312610	8	22	theme	medical	1091:1097	arg1	applications					1099:1110	medical applications	1091:1110	medical applications	1091:1110	These biodegradable composite films can be considered for packaging and medical applications.
27312610	6	23	theme	X-ray	758:762	arg1	diffraction					764:774	X-ray diffraction	758:774	X-ray diffraction	758:774	The cellulose, matrix and the composite films were characterized by Fourier transform infrared spectroscopic, X-ray diffraction, thermogravimetric analysis and SEM techniques.
27312610	3	24	theme	electron	422:429	arg1	SEM					476:478	SEM	476:478	SEM	476:478	Some CuNPs were also formed outside the film in the solution which were separated and viewed by Transmission electron microscope and Scanning electron microscope (SEM).
27312610	3	24	theme	electron	422:429	arg1	microscope					431:440	Transmission electron microscope	409:440	Transmission electron microscope	409:440	Some CuNPs were also formed outside the film in the solution which were separated and viewed by Transmission electron microscope and Scanning electron microscope (SEM).
27312610	0	25	theme	cellulose	15:23	arg1	composites					25:34	cellulose composites	15:34	cellulose composites	15:34	Preparation of cellulose composites with in situ generated copper nanoparticles using leaf extract and their properties.
27312610	6	26	theme	cellulose	652:660	arg1	matrix					663:668	The cellulose, matrix	648:668	matrix	663:668	The cellulose, matrix and the composite films were characterized by Fourier transform infrared spectroscopic, X-ray diffraction, thermogravimetric analysis and SEM techniques.
27312610	7	27	theme	films	862:866	arg1	strength					836:843	The tensile strength	824:843	The tensile strength of the composite films	824:866	The tensile strength of the composite films was lower than that of the matrix but still higher than the conventional polymers like polyethylene and polypropylene used for packaging applications.
27312610	7	27	theme	films	862:866	arg1	lower					872:876	lower	872:876	lower	872:876	The tensile strength of the composite films was lower than that of the matrix but still higher than the conventional polymers like polyethylene and polypropylene used for packaging applications.
27312610	4	28	theme	composite	486:494	arg1	films					496:500	The composite films	482:500	The composite films	482:500	The composite films showed good antibacterial activity against Escherichia coli bacteria when the CuNPs were generated using higher concentrated aq.
27312610	1	29	theme	leaf	235:238	arg1	agent					262:266	a reducing agent	251:266	a reducing agent	251:266	In the present work, copper nanoparticles (CuNPs) were in situ generated in cellulose matrix using Ocimum sanctum leaf extract as a reducing agent and aq.
27312610	1	29	theme	leaf	235:238	arg1	extract					240:246	Ocimum sanctum leaf extract	220:246	Ocimum sanctum leaf extract	220:246	In the present work, copper nanoparticles (CuNPs) were in situ generated in cellulose matrix using Ocimum sanctum leaf extract as a reducing agent and aq.
27312610	3	30	theme	Transmission	409:420	arg1	SEM					476:478	SEM	476:478	SEM	476:478	Some CuNPs were also formed outside the film in the solution which were separated and viewed by Transmission electron microscope and Scanning electron microscope (SEM).
27312610	3	30	theme	Transmission	409:420	arg1	microscope					431:440	Transmission electron microscope	409:440	Transmission electron microscope	409:440	Some CuNPs were also formed outside the film in the solution which were separated and viewed by Transmission electron microscope and Scanning electron microscope (SEM).
27312610	0	31	theme	generated	49:57	arg1	nanoparticles					66:78	in situ generated copper nanoparticles	41:78	in situ generated copper nanoparticles using leaf extract and their properties	41:118	Preparation of cellulose composites with in situ generated copper nanoparticles using leaf extract and their properties.
27312610	1	32	theme	reducing	253:260	arg1	agent					262:266	a reducing agent	251:266	a reducing agent	251:266	In the present work, copper nanoparticles (CuNPs) were in situ generated in cellulose matrix using Ocimum sanctum leaf extract as a reducing agent and aq.
27312610	1	32	theme	reducing	253:260	arg1	extract					240:246	Ocimum sanctum leaf extract	220:246	Ocimum sanctum leaf extract	220:246	In the present work, copper nanoparticles (CuNPs) were in situ generated in cellulose matrix using Ocimum sanctum leaf extract as a reducing agent and aq.
27312610	6	33	theme	spectroscopic	743:755	arg1	techniques					812:821	spectroscopic, X-ray diffraction, thermogravimetric analysis and SEM techniques	743:821	spectroscopic, X-ray diffraction, thermogravimetric analysis and SEM techniques	743:821	The cellulose, matrix and the composite films were characterized by Fourier transform infrared spectroscopic, X-ray diffraction, thermogravimetric analysis and SEM techniques.
27312610	3	34	theme	electron	455:462	arg1	microscope					464:473	Scanning electron microscope	446:473	Scanning electron microscope	446:473	Some CuNPs were also formed outside the film in the solution which were separated and viewed by Transmission electron microscope and Scanning electron microscope (SEM).
27312610	7	35	theme	packaging	995:1003	arg1	applications					1005:1016	packaging applications	995:1016	packaging applications	995:1016	The tensile strength of the composite films was lower than that of the matrix but still higher than the conventional polymers like polyethylene and polypropylene used for packaging applications.
27312610	0	36	with	Preparation	0:10	arg1	nanoparticles					66:78	in situ generated copper nanoparticles	41:78	in situ generated copper nanoparticles using leaf extract and their properties	41:118	Preparation of cellulose composites with in situ generated copper nanoparticles using leaf extract and their properties.
27312610	2	37	theme	diffusion	294:302	arg1	process					304:310	diffusion process	294:310	diffusion process	294:310	CuSO4 solution by diffusion process.
27312610	1	38	theme	cellulose	197:205	arg1	matrix					207:212	cellulose matrix	197:212	cellulose matrix	197:212	In the present work, copper nanoparticles (CuNPs) were in situ generated in cellulose matrix using Ocimum sanctum leaf extract as a reducing agent and aq.
27312610	3	39	theme	Scanning	446:453	arg1	microscope					464:473	Scanning electron microscope	446:473	Scanning electron microscope	446:473	Some CuNPs were also formed outside the film in the solution which were separated and viewed by Transmission electron microscope and Scanning electron microscope (SEM).
27312610	6	40	dep	Fourier	716:722	arg1	transform					724:732	transform	724:732	transform infrared spectroscopic, X-ray diffraction, thermogravimetric analysis and SEM techniques	724:821	The cellulose, matrix and the composite films were characterized by Fourier transform infrared spectroscopic, X-ray diffraction, thermogravimetric analysis and SEM techniques.
27312610	4	41	theme	concentrated	614:625	arg1	aq					627:628	higher concentrated aq	607:628	higher concentrated aq	607:628	The composite films showed good antibacterial activity against Escherichia coli bacteria when the CuNPs were generated using higher concentrated aq.
27312610	4	42	theme	higher	607:612	arg1	aq					627:628	higher concentrated aq	607:628	higher concentrated aq	607:628	The composite films showed good antibacterial activity against Escherichia coli bacteria when the CuNPs were generated using higher concentrated aq.
27312610	0	43	theme	copper	59:64	arg1	nanoparticles					66:78	in situ generated copper nanoparticles	41:78	in situ generated copper nanoparticles using leaf extract and their properties	41:118	Preparation of cellulose composites with in situ generated copper nanoparticles using leaf extract and their properties.
27312610	1	44	theme	Ocimum	220:225	arg1	agent					262:266	a reducing agent	251:266	a reducing agent	251:266	In the present work, copper nanoparticles (CuNPs) were in situ generated in cellulose matrix using Ocimum sanctum leaf extract as a reducing agent and aq.
27312610	1	44	theme	Ocimum	220:225	arg1	extract					240:246	Ocimum sanctum leaf extract	220:246	Ocimum sanctum leaf extract	220:246	In the present work, copper nanoparticles (CuNPs) were in situ generated in cellulose matrix using Ocimum sanctum leaf extract as a reducing agent and aq.
29254036	3	0	theme	film	766:769	arg1	growth					771:776	the exponential film growth	750:776	the exponential film growth to linear	750:786	It was found that when deposited in-between the HA and Chi layers GO forms diffusion barrier, hindering the mobility of Chi-chains and changing the exponential film growth to linear.
29254036	4	1	theme	dramatic	920:927	arg1	impact					929:934	a dramatic impact	918:934	a dramatic impact on the surface morphology and roughness, which in turn tunes the hydrophilicity, protein adsorption and platelets adhesion	918:1057	Incorporation of GO-layers into the biodegradable and highly hydrated HA/Chi matrix does not affect the final thickness, but has a dramatic impact on the surface morphology and roughness, which in turn tunes the hydrophilicity, protein adsorption and platelets adhesion.
29254036	1	2	theme	oxide/hyaluronan/chitosan	187:211	arg1	multilayers					223:233	hybrid graphene oxide/hyaluronan/chitosan GO/HA/Chi multilayers	171:233	hybrid graphene oxide/hyaluronan/chitosan GO/HA/Chi multilayers	171:233	Herein, a strong interdependence between the composition of hybrid graphene oxide/hyaluronan/chitosan GO/HA/Chi multilayers and their surface properties and biocompatibility was demonstrated that can be used to build up coatings with desirable and precisely tunable properties.
29254036	4	3	theme	adsorption	1025:1034	arg1	adhesion					1050:1057	the hydrophilicity, protein adsorption and platelets adhesion	997:1057	the hydrophilicity, protein adsorption and platelets adhesion	997:1057	Incorporation of GO-layers into the biodegradable and highly hydrated HA/Chi matrix does not affect the final thickness, but has a dramatic impact on the surface morphology and roughness, which in turn tunes the hydrophilicity, protein adsorption and platelets adhesion.
29254036	1	4	theme	GO/HA/Chi	213:221	arg1	multilayers					223:233	hybrid graphene oxide/hyaluronan/chitosan GO/HA/Chi multilayers	171:233	hybrid graphene oxide/hyaluronan/chitosan GO/HA/Chi multilayers	171:233	Herein, a strong interdependence between the composition of hybrid graphene oxide/hyaluronan/chitosan GO/HA/Chi multilayers and their surface properties and biocompatibility was demonstrated that can be used to build up coatings with desirable and precisely tunable properties.
29254036	4	5	theme	hydrated	850:857	arg1	matrix					866:871	the biodegradable and highly hydrated HA/Chi matrix	821:871	the biodegradable and highly hydrated HA/Chi matrix	821:871	Incorporation of GO-layers into the biodegradable and highly hydrated HA/Chi matrix does not affect the final thickness, but has a dramatic impact on the surface morphology and roughness, which in turn tunes the hydrophilicity, protein adsorption and platelets adhesion.
29254036	4	6	theme	biodegradable	825:837	arg1	matrix					866:871	the biodegradable and highly hydrated HA/Chi matrix	821:871	the biodegradable and highly hydrated HA/Chi matrix	821:871	Incorporation of GO-layers into the biodegradable and highly hydrated HA/Chi matrix does not affect the final thickness, but has a dramatic impact on the surface morphology and roughness, which in turn tunes the hydrophilicity, protein adsorption and platelets adhesion.
29254036	4	7	theme	hydrophilicity	1001:1014	arg1	adhesion					1050:1057	the hydrophilicity, protein adsorption and platelets adhesion	997:1057	the hydrophilicity, protein adsorption and platelets adhesion	997:1057	Incorporation of GO-layers into the biodegradable and highly hydrated HA/Chi matrix does not affect the final thickness, but has a dramatic impact on the surface morphology and roughness, which in turn tunes the hydrophilicity, protein adsorption and platelets adhesion.
29254036	1	8	theme	multilayers	223:233	arg1	composition					156:166	the composition	152:166	the composition of hybrid graphene oxide/hyaluronan/chitosan GO/HA/Chi multilayers	152:233	Herein, a strong interdependence between the composition of hybrid graphene oxide/hyaluronan/chitosan GO/HA/Chi multilayers and their surface properties and biocompatibility was demonstrated that can be used to build up coatings with desirable and precisely tunable properties.
29254036	1	8	theme	multilayers	223:233	arg1	biocompatibility					268:283	biocompatibility	268:283	biocompatibility	268:283	Herein, a strong interdependence between the composition of hybrid graphene oxide/hyaluronan/chitosan GO/HA/Chi multilayers and their surface properties and biocompatibility was demonstrated that can be used to build up coatings with desirable and precisely tunable properties.
29254036	1	8	theme	multilayers	223:233	arg1	properties					253:262	their surface properties	239:262	their surface properties	239:262	Herein, a strong interdependence between the composition of hybrid graphene oxide/hyaluronan/chitosan GO/HA/Chi multilayers and their surface properties and biocompatibility was demonstrated that can be used to build up coatings with desirable and precisely tunable properties.
29254036	0	9	theme	surface	70:76	arg1	properties					78:87	controllable surface properties	57:87	controllable surface properties	57:87	Hybrid graphene oxide/polysaccharide nanocomposites with controllable surface properties and biocompatibility.
29254036	5	10	theme	surface	1183:1189	arg1	properties					1191:1200	controllable surface properties	1170:1200	controllable surface properties	1170:1200	This provides an opportunity for various biomedical applications of the studied hybrid films as coatings with controllable surface properties.
29254036	2	11	theme	polymer	447:453	arg1	matrix					455:460	the polymer matrix	443:460	the polymer matrix	443:460	Both the position and the abundance of GO-layers into the polymer matrix were systematically varied to draw interconnection with the growth type, thickness, morphology, roughness, hydrophilicity and biocompatibility.
29254036	0	12	theme	graphene	7:14	arg1	oxide/polysaccharide					16:35	Hybrid graphene oxide/polysaccharide	0:35	Hybrid graphene oxide/polysaccharide	0:35	Hybrid graphene oxide/polysaccharide nanocomposites with controllable surface properties and biocompatibility.
29254036	5	13	theme	controllable	1170:1181	arg1	properties					1191:1200	controllable surface properties	1170:1200	controllable surface properties	1170:1200	This provides an opportunity for various biomedical applications of the studied hybrid films as coatings with controllable surface properties.
29254036	0	14	theme	Hybrid	0:5	arg1	oxide/polysaccharide					16:35	Hybrid graphene oxide/polysaccharide	0:35	Hybrid graphene oxide/polysaccharide	0:35	Hybrid graphene oxide/polysaccharide nanocomposites with controllable surface properties and biocompatibility.
29254036	1	15	with	coatings	331:338	arg1	properties					377:386	desirable and precisely tunable properties	345:386	desirable and precisely tunable properties	345:386	Herein, a strong interdependence between the composition of hybrid graphene oxide/hyaluronan/chitosan GO/HA/Chi multilayers and their surface properties and biocompatibility was demonstrated that can be used to build up coatings with desirable and precisely tunable properties.
29254036	3	16	theme	in-between	639:648	arg1	layers					665:670	in-between the HA and Chi layers	639:670	in-between the HA and Chi layers	639:670	It was found that when deposited in-between the HA and Chi layers GO forms diffusion barrier, hindering the mobility of Chi-chains and changing the exponential film growth to linear.
29254036	1	17	theme	surface	245:251	arg1	properties					253:262	their surface properties	239:262	their surface properties	239:262	Herein, a strong interdependence between the composition of hybrid graphene oxide/hyaluronan/chitosan GO/HA/Chi multilayers and their surface properties and biocompatibility was demonstrated that can be used to build up coatings with desirable and precisely tunable properties.
29254036	1	18	theme	strong	121:126	arg1	interdependence					128:142	a strong interdependence	119:142	a strong interdependence between the composition of hybrid graphene oxide/hyaluronan/chitosan GO/HA/Chi multilayers and their surface properties and biocompatibility	119:283	Herein, a strong interdependence between the composition of hybrid graphene oxide/hyaluronan/chitosan GO/HA/Chi multilayers and their surface properties and biocompatibility was demonstrated that can be used to build up coatings with desirable and precisely tunable properties.
29254036	1	19	theme	desirable	345:353	arg1	properties					377:386	desirable and precisely tunable properties	345:386	desirable and precisely tunable properties	345:386	Herein, a strong interdependence between the composition of hybrid graphene oxide/hyaluronan/chitosan GO/HA/Chi multilayers and their surface properties and biocompatibility was demonstrated that can be used to build up coatings with desirable and precisely tunable properties.
29254036	4	20	theme	GO-layers	806:814	arg1	Incorporation					789:801	Incorporation	789:801	Incorporation of GO-layers into the biodegradable and highly hydrated HA/Chi matrix	789:871	Incorporation of GO-layers into the biodegradable and highly hydrated HA/Chi matrix does not affect the final thickness, but has a dramatic impact on the surface morphology and roughness, which in turn tunes the hydrophilicity, protein adsorption and platelets adhesion.
29254036	1	21	theme	hybrid	171:176	arg1	multilayers					223:233	hybrid graphene oxide/hyaluronan/chitosan GO/HA/Chi multilayers	171:233	hybrid graphene oxide/hyaluronan/chitosan GO/HA/Chi multilayers	171:233	Herein, a strong interdependence between the composition of hybrid graphene oxide/hyaluronan/chitosan GO/HA/Chi multilayers and their surface properties and biocompatibility was demonstrated that can be used to build up coatings with desirable and precisely tunable properties.
29254036	0	22	theme	controllable	57:68	arg1	properties					78:87	controllable surface properties	57:87	controllable surface properties	57:87	Hybrid graphene oxide/polysaccharide nanocomposites with controllable surface properties and biocompatibility.
29254036	5	23	theme	studied	1132:1138	arg1	films					1147:1151	the studied hybrid films	1128:1151	the studied hybrid films as coatings with controllable surface properties	1128:1200	This provides an opportunity for various biomedical applications of the studied hybrid films as coatings with controllable surface properties.
29254036	5	24	theme	various	1093:1099	arg1	applications					1112:1123	various biomedical applications	1093:1123	various biomedical applications of the studied hybrid films as coatings with controllable surface properties	1093:1200	This provides an opportunity for various biomedical applications of the studied hybrid films as coatings with controllable surface properties.
29254036	5	25	with	coatings	1156:1163	arg1	properties					1191:1200	controllable surface properties	1170:1200	controllable surface properties	1170:1200	This provides an opportunity for various biomedical applications of the studied hybrid films as coatings with controllable surface properties.
29254036	3	26	dep	forms	675:679	arg1	hindering					700:708	hindering	700:708	hindering the mobility of Chi-chains	700:735	It was found that when deposited in-between the HA and Chi layers GO forms diffusion barrier, hindering the mobility of Chi-chains and changing the exponential film growth to linear.
29254036	3	26	dep	forms	675:679	arg1	changing					741:748	changing	741:748	changing the exponential film growth to linear	741:786	It was found that when deposited in-between the HA and Chi layers GO forms diffusion barrier, hindering the mobility of Chi-chains and changing the exponential film growth to linear.
29254036	4	27	theme	platelets	1040:1048	arg1	adhesion					1050:1057	the hydrophilicity, protein adsorption and platelets adhesion	997:1057	the hydrophilicity, protein adsorption and platelets adhesion	997:1057	Incorporation of GO-layers into the biodegradable and highly hydrated HA/Chi matrix does not affect the final thickness, but has a dramatic impact on the surface morphology and roughness, which in turn tunes the hydrophilicity, protein adsorption and platelets adhesion.
29254036	5	28	theme	hybrid	1140:1145	arg1	films					1147:1151	the studied hybrid films	1128:1151	the studied hybrid films as coatings with controllable surface properties	1128:1200	This provides an opportunity for various biomedical applications of the studied hybrid films as coatings with controllable surface properties.
29254036	3	29	theme	Chi	661:663	arg1	layers					665:670	in-between the HA and Chi layers	639:670	in-between the HA and Chi layers	639:670	It was found that when deposited in-between the HA and Chi layers GO forms diffusion barrier, hindering the mobility of Chi-chains and changing the exponential film growth to linear.
29254036	1	30	theme	tunable	369:375	arg1	properties					377:386	desirable and precisely tunable properties	345:386	desirable and precisely tunable properties	345:386	Herein, a strong interdependence between the composition of hybrid graphene oxide/hyaluronan/chitosan GO/HA/Chi multilayers and their surface properties and biocompatibility was demonstrated that can be used to build up coatings with desirable and precisely tunable properties.
29254036	4	31	theme	surface	943:949	arg1	morphology					951:960	surface morphology	943:960	surface morphology	943:960	Incorporation of GO-layers into the biodegradable and highly hydrated HA/Chi matrix does not affect the final thickness, but has a dramatic impact on the surface morphology and roughness, which in turn tunes the hydrophilicity, protein adsorption and platelets adhesion.
29254036	5	32	theme	films	1147:1151	arg1	applications					1112:1123	various biomedical applications	1093:1123	various biomedical applications of the studied hybrid films as coatings with controllable surface properties	1093:1200	This provides an opportunity for various biomedical applications of the studied hybrid films as coatings with controllable surface properties.
29254036	4	33	theme	final	893:897	arg1	thickness					899:907	the final thickness	889:907	the final thickness	889:907	Incorporation of GO-layers into the biodegradable and highly hydrated HA/Chi matrix does not affect the final thickness, but has a dramatic impact on the surface morphology and roughness, which in turn tunes the hydrophilicity, protein adsorption and platelets adhesion.
29254036	2	34	theme	growth	522:527	arg1	type					529:532	the growth type	518:532	the growth type	518:532	Both the position and the abundance of GO-layers into the polymer matrix were systematically varied to draw interconnection with the growth type, thickness, morphology, roughness, hydrophilicity and biocompatibility.
29254036	4	35	from	impact	929:934	arg1	morphology					951:960	surface morphology	943:960	surface morphology	943:960	Incorporation of GO-layers into the biodegradable and highly hydrated HA/Chi matrix does not affect the final thickness, but has a dramatic impact on the surface morphology and roughness, which in turn tunes the hydrophilicity, protein adsorption and platelets adhesion.
29254036	4	35	from	impact	929:934	arg1	roughness					966:974	roughness	966:974	roughness	966:974	Incorporation of GO-layers into the biodegradable and highly hydrated HA/Chi matrix does not affect the final thickness, but has a dramatic impact on the surface morphology and roughness, which in turn tunes the hydrophilicity, protein adsorption and platelets adhesion.
29254036	3	36	theme	Chi-chains	726:735	arg1	mobility					714:721	the mobility	710:721	the mobility of Chi-chains	710:735	It was found that when deposited in-between the HA and Chi layers GO forms diffusion barrier, hindering the mobility of Chi-chains and changing the exponential film growth to linear.
29254036	3	37	theme	HA	654:655	arg1	layers					665:670	in-between the HA and Chi layers	639:670	in-between the HA and Chi layers	639:670	It was found that when deposited in-between the HA and Chi layers GO forms diffusion barrier, hindering the mobility of Chi-chains and changing the exponential film growth to linear.
29254036	5	38	theme	biomedical	1101:1110	arg1	applications					1112:1123	various biomedical applications	1093:1123	various biomedical applications of the studied hybrid films as coatings with controllable surface properties	1093:1200	This provides an opportunity for various biomedical applications of the studied hybrid films as coatings with controllable surface properties.
29254036	4	39	theme	HA/Chi	859:864	arg1	matrix					866:871	the biodegradable and highly hydrated HA/Chi matrix	821:871	the biodegradable and highly hydrated HA/Chi matrix	821:871	Incorporation of GO-layers into the biodegradable and highly hydrated HA/Chi matrix does not affect the final thickness, but has a dramatic impact on the surface morphology and roughness, which in turn tunes the hydrophilicity, protein adsorption and platelets adhesion.
29254036	4	40	contain	has	914:916	arg1	Incorporation					789:801	Incorporation	789:801	Incorporation of GO-layers into the biodegradable and highly hydrated HA/Chi matrix	789:871	Incorporation of GO-layers into the biodegradable and highly hydrated HA/Chi matrix does not affect the final thickness, but has a dramatic impact on the surface morphology and roughness, which in turn tunes the hydrophilicity, protein adsorption and platelets adhesion.
29254036	4	40	contain	has	914:916	arg2	impact					929:934	a dramatic impact	918:934	a dramatic impact on the surface morphology and roughness, which in turn tunes the hydrophilicity, protein adsorption and platelets adhesion	918:1057	Incorporation of GO-layers into the biodegradable and highly hydrated HA/Chi matrix does not affect the final thickness, but has a dramatic impact on the surface morphology and roughness, which in turn tunes the hydrophilicity, protein adsorption and platelets adhesion.
29254036	3	41	theme	diffusion	681:689	arg1	barrier					691:697	diffusion barrier	681:697	diffusion barrier	681:697	It was found that when deposited in-between the HA and Chi layers GO forms diffusion barrier, hindering the mobility of Chi-chains and changing the exponential film growth to linear.
29254036	4	42	theme	protein	1017:1023	arg1	adsorption					1025:1034	protein adsorption	1017:1034	protein adsorption	1017:1034	Incorporation of GO-layers into the biodegradable and highly hydrated HA/Chi matrix does not affect the final thickness, but has a dramatic impact on the surface morphology and roughness, which in turn tunes the hydrophilicity, protein adsorption and platelets adhesion.
29254036	2	43	theme	GO-layers	428:436	arg1	position					398:405	the position	394:405	the position	394:405	Both the position and the abundance of GO-layers into the polymer matrix were systematically varied to draw interconnection with the growth type, thickness, morphology, roughness, hydrophilicity and biocompatibility.
29254036	2	43	theme	GO-layers	428:436	arg1	abundance					415:423	the abundance	411:423	the abundance of GO-layers into the polymer matrix	411:460	Both the position and the abundance of GO-layers into the polymer matrix were systematically varied to draw interconnection with the growth type, thickness, morphology, roughness, hydrophilicity and biocompatibility.
29254036	3	44	dep	HA	654:655	arg1	the					650:652	the	650:652	the	650:652	It was found that when deposited in-between the HA and Chi layers GO forms diffusion barrier, hindering the mobility of Chi-chains and changing the exponential film growth to linear.
29254036	4	45	dep	morphology	951:960	arg1	the					939:941	the	939:941	the	939:941	Incorporation of GO-layers into the biodegradable and highly hydrated HA/Chi matrix does not affect the final thickness, but has a dramatic impact on the surface morphology and roughness, which in turn tunes the hydrophilicity, protein adsorption and platelets adhesion.
29254036	3	46	theme	exponential	754:764	arg1	growth					771:776	the exponential film growth	750:776	the exponential film growth to linear	750:786	It was found that when deposited in-between the HA and Chi layers GO forms diffusion barrier, hindering the mobility of Chi-chains and changing the exponential film growth to linear.
29254036	1	47	theme	graphene	178:185	arg1	multilayers					223:233	hybrid graphene oxide/hyaluronan/chitosan GO/HA/Chi multilayers	171:233	hybrid graphene oxide/hyaluronan/chitosan GO/HA/Chi multilayers	171:233	Herein, a strong interdependence between the composition of hybrid graphene oxide/hyaluronan/chitosan GO/HA/Chi multilayers and their surface properties and biocompatibility was demonstrated that can be used to build up coatings with desirable and precisely tunable properties.
28115086	7	0	theme	antineoplastic	1020:1033	arg1	effect					1035:1040	The antineoplastic effect	1016:1040	The antineoplastic effect of SFP	1016:1047	The antineoplastic effect of SFP appears to be attributable to its action on the immune system by controlling the tumor microenvironment and stimulating TNF-α production, which may trigger the necroptosis pathway.
28115086	7	0	theme	antineoplastic	1020:1033	arg1	attributable					1063:1074	attributable	1063:1074	attributable	1063:1074	The antineoplastic effect of SFP appears to be attributable to its action on the immune system by controlling the tumor microenvironment and stimulating TNF-α production, which may trigger the necroptosis pathway.
28115086	6	1	theme	necroptosis-related	975:993	arg1	Rip1					1001:1004	Rip1	1001:1004	Rip1	1001:1004	Treatment with SFP also induced the expression of the cell necroptosis-related genes Rip1 and Rip3.
28115086	6	1	theme	necroptosis-related	975:993	arg1	genes					995:999	the cell necroptosis-related genes Rip1 and Rip3	966:1013	the cell necroptosis-related genes Rip1 and Rip3	966:1013	Treatment with SFP also induced the expression of the cell necroptosis-related genes Rip1 and Rip3.
28115086	6	1	theme	necroptosis-related	975:993	arg1	Rip3					1010:1013	Rip3	1010:1013	Rip3	1010:1013	Treatment with SFP also induced the expression of the cell necroptosis-related genes Rip1 and Rip3.
28115086	0	2	theme	polysaccharide	75:88	arg1	fraction					63:70	the soluble fraction	51:70	the soluble fraction of polysaccharide	51:88	Necroptosis mediates the antineoplastic effects of the soluble fraction of polysaccharide from red wine in Walker-256 tumor-bearing rats.
28115086	4	3	dep	SFP	540:542	arg1	60mg/kg					552:558	60mg/kg	552:558	60mg/kg	552:558	Treatment with SFP (30 and 60mg/kg, oral) for 14days significantly reduced the tumor weight and volume compared with controls.
28115086	4	3	dep	SFP	540:542	arg1	30					545:546	30	545:546	30	545:546	Treatment with SFP (30 and 60mg/kg, oral) for 14days significantly reduced the tumor weight and volume compared with controls.
28115086	7	4	theme	immune	1097:1102	arg1	system					1104:1109	the immune system	1093:1109	the immune system	1093:1109	The antineoplastic effect of SFP appears to be attributable to its action on the immune system by controlling the tumor microenvironment and stimulating TNF-α production, which may trigger the necroptosis pathway.
28115086	2	5	theme	red	361:363	arg1	wine					365:368	cabernet franc red wine	346:368	cabernet franc red wine	346:368	The present study investigated the antitumor activity of the soluble fraction of polysaccharides (SFP), extracted from cabernet franc red wine, in Walker-256 tumor-bearing rats.
28115086	6	6	theme	cell	970:973	arg1	Rip1					1001:1004	Rip1	1001:1004	Rip1	1001:1004	Treatment with SFP also induced the expression of the cell necroptosis-related genes Rip1 and Rip3.
28115086	6	6	theme	cell	970:973	arg1	genes					995:999	the cell necroptosis-related genes Rip1 and Rip3	966:1013	the cell necroptosis-related genes Rip1 and Rip3	966:1013	Treatment with SFP also induced the expression of the cell necroptosis-related genes Rip1 and Rip3.
28115086	6	6	theme	cell	970:973	arg1	Rip3					1010:1013	Rip3	1010:1013	Rip3	1010:1013	Treatment with SFP also induced the expression of the cell necroptosis-related genes Rip1 and Rip3.
28115086	2	7	theme	polysaccharides	308:322	arg1	fraction					296:303	the soluble fraction	284:303	the soluble fraction of polysaccharides (SFP), extracted from cabernet franc red wine,	284:369	The present study investigated the antitumor activity of the soluble fraction of polysaccharides (SFP), extracted from cabernet franc red wine, in Walker-256 tumor-bearing rats.
28115086	0	8	theme	red	95:97	arg1	wine					99:102	red wine	95:102	red wine in Walker-256 tumor-bearing rats	95:135	Necroptosis mediates the antineoplastic effects of the soluble fraction of polysaccharide from red wine in Walker-256 tumor-bearing rats.
28115086	3	9	contain	had	436:438	arg1	composition					424:434	The monosaccharide composition	405:434	The monosaccharide composition	405:434	The monosaccharide composition had a complex mixture, suggesting the presence of arabinoglactans, mannans, and pectins.
28115086	3	9	contain	had	436:438	arg2	mixture					450:456	a complex mixture	440:456	a complex mixture	440:456	The monosaccharide composition had a complex mixture, suggesting the presence of arabinoglactans, mannans, and pectins.
28115086	4	10	with	Treatment	525:533	arg1	SFP					540:542	SFP	540:542	SFP (30 and 60mg/kg, oral)	540:565	Treatment with SFP (30 and 60mg/kg, oral) for 14days significantly reduced the tumor weight and volume compared with controls.
28115086	6	11	theme	genes	995:999	arg1	expression					952:961	the expression	948:961	the expression of the cell necroptosis-related genes Rip1 and Rip3	948:1013	Treatment with SFP also induced the expression of the cell necroptosis-related genes Rip1 and Rip3.
28115086	5	12	theme	tumor	732:736	arg1	activity					738:745	the tumor activity	728:745	the tumor activity of N-acetylglucosaminidase, myeloperoxidase, and nitric oxide	728:807	Treatment with 60mg/kg SFP reduced blood monocytes and neutrophils, reduced the tumor activity of N-acetylglucosaminidase, myeloperoxidase, and nitric oxide, increased blood lymphocytes, and increased the levels of tumor necrosis factor α (TNF-α) in tumor tissue.
28115086	3	13	theme	mannans	503:509	arg1	presence					474:481	the presence	470:481	the presence of arabinoglactans, mannans, and pectins	470:522	The monosaccharide composition had a complex mixture, suggesting the presence of arabinoglactans, mannans, and pectins.
28115086	2	14	theme	fraction	296:303	arg1	activity					272:279	the antitumor activity	258:279	the antitumor activity of the soluble fraction of polysaccharides (SFP), extracted from cabernet franc red wine, in Walker-256 tumor-bearing rats	258:402	The present study investigated the antitumor activity of the soluble fraction of polysaccharides (SFP), extracted from cabernet franc red wine, in Walker-256 tumor-bearing rats.
28115086	5	15	theme	tumor	867:871	arg1	factor					882:887	tumor necrosis factor α	867:889	tumor necrosis factor α (TNF-α)	867:897	Treatment with 60mg/kg SFP reduced blood monocytes and neutrophils, reduced the tumor activity of N-acetylglucosaminidase, myeloperoxidase, and nitric oxide, increased blood lymphocytes, and increased the levels of tumor necrosis factor α (TNF-α) in tumor tissue.
28115086	5	15	theme	tumor	867:871	arg1	TNF-α					892:896	TNF-α	892:896	TNF-α	892:896	Treatment with 60mg/kg SFP reduced blood monocytes and neutrophils, reduced the tumor activity of N-acetylglucosaminidase, myeloperoxidase, and nitric oxide, increased blood lymphocytes, and increased the levels of tumor necrosis factor α (TNF-α) in tumor tissue.
28115086	5	16	theme	oxide	803:807	arg1	activity					738:745	the tumor activity	728:745	the tumor activity of N-acetylglucosaminidase, myeloperoxidase, and nitric oxide	728:807	Treatment with 60mg/kg SFP reduced blood monocytes and neutrophils, reduced the tumor activity of N-acetylglucosaminidase, myeloperoxidase, and nitric oxide, increased blood lymphocytes, and increased the levels of tumor necrosis factor α (TNF-α) in tumor tissue.
28115086	2	17	theme	soluble	288:294	arg1	fraction					296:303	the soluble fraction	284:303	the soluble fraction of polysaccharides (SFP), extracted from cabernet franc red wine,	284:369	The present study investigated the antitumor activity of the soluble fraction of polysaccharides (SFP), extracted from cabernet franc red wine, in Walker-256 tumor-bearing rats.
28115086	1	18	theme	biological	185:194	arg1	response					196:203	the biological response	181:203	the biological response to several stressors	181:224	Polysaccharides are substances that modify the biological response to several stressors.
28115086	5	19	theme	myeloperoxidase	775:789	arg1	activity					738:745	the tumor activity	728:745	the tumor activity of N-acetylglucosaminidase, myeloperoxidase, and nitric oxide	728:807	Treatment with 60mg/kg SFP reduced blood monocytes and neutrophils, reduced the tumor activity of N-acetylglucosaminidase, myeloperoxidase, and nitric oxide, increased blood lymphocytes, and increased the levels of tumor necrosis factor α (TNF-α) in tumor tissue.
28115086	6	20	with	Treatment	916:924	arg1	SFP					931:933	SFP	931:933	SFP	931:933	Treatment with SFP also induced the expression of the cell necroptosis-related genes Rip1 and Rip3.
28115086	6	21	dep	genes	995:999	arg1	Rip1					1001:1004	Rip1	1001:1004	Rip1	1001:1004	Treatment with SFP also induced the expression of the cell necroptosis-related genes Rip1 and Rip3.
28115086	6	21	dep	genes	995:999	arg1	genes					995:999	the cell necroptosis-related genes Rip1 and Rip3	966:1013	the cell necroptosis-related genes Rip1 and Rip3	966:1013	Treatment with SFP also induced the expression of the cell necroptosis-related genes Rip1 and Rip3.
28115086	6	21	dep	genes	995:999	arg1	Rip3					1010:1013	Rip3	1010:1013	Rip3	1010:1013	Treatment with SFP also induced the expression of the cell necroptosis-related genes Rip1 and Rip3.
28115086	2	22	theme	tumor-bearing	385:397	arg1	rats					399:402	Walker-256 tumor-bearing rats	374:402	Walker-256 tumor-bearing rats	374:402	The present study investigated the antitumor activity of the soluble fraction of polysaccharides (SFP), extracted from cabernet franc red wine, in Walker-256 tumor-bearing rats.
28115086	2	23	from	activity	272:279	arg1	rats					399:402	Walker-256 tumor-bearing rats	374:402	Walker-256 tumor-bearing rats	374:402	The present study investigated the antitumor activity of the soluble fraction of polysaccharides (SFP), extracted from cabernet franc red wine, in Walker-256 tumor-bearing rats.
28115086	3	24	theme	complex	442:448	arg1	mixture					450:456	a complex mixture	440:456	a complex mixture	440:456	The monosaccharide composition had a complex mixture, suggesting the presence of arabinoglactans, mannans, and pectins.
28115086	0	25	theme	antineoplastic	25:38	arg1	effects					40:46	the antineoplastic effects	21:46	the antineoplastic effects of the soluble fraction of polysaccharide from red wine in Walker-256 tumor-bearing rats	21:135	Necroptosis mediates the antineoplastic effects of the soluble fraction of polysaccharide from red wine in Walker-256 tumor-bearing rats.
28115086	5	26	theme	nitric	796:801	arg1	oxide					803:807	nitric oxide	796:807	nitric oxide	796:807	Treatment with 60mg/kg SFP reduced blood monocytes and neutrophils, reduced the tumor activity of N-acetylglucosaminidase, myeloperoxidase, and nitric oxide, increased blood lymphocytes, and increased the levels of tumor necrosis factor α (TNF-α) in tumor tissue.
28115086	1	27	theme	several	208:214	arg1	stressors					216:224	several stressors	208:224	several stressors	208:224	Polysaccharides are substances that modify the biological response to several stressors.
28115086	0	28	theme	tumor-bearing	118:130	arg1	rats					132:135	Walker-256 tumor-bearing rats	107:135	Walker-256 tumor-bearing rats	107:135	Necroptosis mediates the antineoplastic effects of the soluble fraction of polysaccharide from red wine in Walker-256 tumor-bearing rats.
28115086	3	29	theme	pectins	516:522	arg1	presence					474:481	the presence	470:481	the presence of arabinoglactans, mannans, and pectins	470:522	The monosaccharide composition had a complex mixture, suggesting the presence of arabinoglactans, mannans, and pectins.
28115086	5	30	with	Treatment	652:660	arg1	SFP					675:677	60mg/kg SFP	667:677	60mg/kg SFP	667:677	Treatment with 60mg/kg SFP reduced blood monocytes and neutrophils, reduced the tumor activity of N-acetylglucosaminidase, myeloperoxidase, and nitric oxide, increased blood lymphocytes, and increased the levels of tumor necrosis factor α (TNF-α) in tumor tissue.
28115086	7	31	theme	TNF-α	1169:1173	arg1	production					1175:1184	TNF-α production	1169:1184	TNF-α production	1169:1184	The antineoplastic effect of SFP appears to be attributable to its action on the immune system by controlling the tumor microenvironment and stimulating TNF-α production, which may trigger the necroptosis pathway.
28115086	5	32	theme	necrosis	873:880	arg1	factor					882:887	tumor necrosis factor α	867:889	tumor necrosis factor α (TNF-α)	867:897	Treatment with 60mg/kg SFP reduced blood monocytes and neutrophils, reduced the tumor activity of N-acetylglucosaminidase, myeloperoxidase, and nitric oxide, increased blood lymphocytes, and increased the levels of tumor necrosis factor α (TNF-α) in tumor tissue.
28115086	5	32	theme	necrosis	873:880	arg1	TNF-α					892:896	TNF-α	892:896	TNF-α	892:896	Treatment with 60mg/kg SFP reduced blood monocytes and neutrophils, reduced the tumor activity of N-acetylglucosaminidase, myeloperoxidase, and nitric oxide, increased blood lymphocytes, and increased the levels of tumor necrosis factor α (TNF-α) in tumor tissue.
28115086	7	33	from	action	1083:1088	arg1	system					1104:1109	the immune system	1093:1109	the immune system	1093:1109	The antineoplastic effect of SFP appears to be attributable to its action on the immune system by controlling the tumor microenvironment and stimulating TNF-α production, which may trigger the necroptosis pathway.
28115086	7	34	theme	SFP	1045:1047	arg1	effect					1035:1040	The antineoplastic effect	1016:1040	The antineoplastic effect of SFP	1016:1047	The antineoplastic effect of SFP appears to be attributable to its action on the immune system by controlling the tumor microenvironment and stimulating TNF-α production, which may trigger the necroptosis pathway.
28115086	7	34	theme	SFP	1045:1047	arg1	attributable					1063:1074	attributable	1063:1074	attributable	1063:1074	The antineoplastic effect of SFP appears to be attributable to its action on the immune system by controlling the tumor microenvironment and stimulating TNF-α production, which may trigger the necroptosis pathway.
28115086	5	35	theme	blood	820:824	arg1	lymphocytes					826:836	blood lymphocytes	820:836	blood lymphocytes	820:836	Treatment with 60mg/kg SFP reduced blood monocytes and neutrophils, reduced the tumor activity of N-acetylglucosaminidase, myeloperoxidase, and nitric oxide, increased blood lymphocytes, and increased the levels of tumor necrosis factor α (TNF-α) in tumor tissue.
28115086	0	36	theme	soluble	55:61	arg1	fraction					63:70	the soluble fraction	51:70	the soluble fraction of polysaccharide	51:88	Necroptosis mediates the antineoplastic effects of the soluble fraction of polysaccharide from red wine in Walker-256 tumor-bearing rats.
28115086	5	37	theme	60mg/kg	667:673	arg1	SFP					675:677	60mg/kg SFP	667:677	60mg/kg SFP	667:677	Treatment with 60mg/kg SFP reduced blood monocytes and neutrophils, reduced the tumor activity of N-acetylglucosaminidase, myeloperoxidase, and nitric oxide, increased blood lymphocytes, and increased the levels of tumor necrosis factor α (TNF-α) in tumor tissue.
28115086	5	38	theme	N-acetylglucosaminidase	750:772	arg1	activity					738:745	the tumor activity	728:745	the tumor activity of N-acetylglucosaminidase, myeloperoxidase, and nitric oxide	728:807	Treatment with 60mg/kg SFP reduced blood monocytes and neutrophils, reduced the tumor activity of N-acetylglucosaminidase, myeloperoxidase, and nitric oxide, increased blood lymphocytes, and increased the levels of tumor necrosis factor α (TNF-α) in tumor tissue.
28115086	2	39	theme	franc	355:359	arg1	wine					365:368	cabernet franc red wine	346:368	cabernet franc red wine	346:368	The present study investigated the antitumor activity of the soluble fraction of polysaccharides (SFP), extracted from cabernet franc red wine, in Walker-256 tumor-bearing rats.
28115086	3	40	theme	monosaccharide	409:422	arg1	composition					424:434	The monosaccharide composition	405:434	The monosaccharide composition	405:434	The monosaccharide composition had a complex mixture, suggesting the presence of arabinoglactans, mannans, and pectins.
28115086	2	41	theme	present	231:237	arg1	study					239:243	The present study	227:243	The present study	227:243	The present study investigated the antitumor activity of the soluble fraction of polysaccharides (SFP), extracted from cabernet franc red wine, in Walker-256 tumor-bearing rats.
28115086	2	42	theme	cabernet	346:353	arg1	wine					365:368	cabernet franc red wine	346:368	cabernet franc red wine	346:368	The present study investigated the antitumor activity of the soluble fraction of polysaccharides (SFP), extracted from cabernet franc red wine, in Walker-256 tumor-bearing rats.
28115086	7	43	theme	tumor	1130:1134	arg1	microenvironment					1136:1151	the tumor microenvironment	1126:1151	the tumor microenvironment	1126:1151	The antineoplastic effect of SFP appears to be attributable to its action on the immune system by controlling the tumor microenvironment and stimulating TNF-α production, which may trigger the necroptosis pathway.
28115086	3	44	theme	arabinoglactans	486:500	arg1	presence					474:481	the presence	470:481	the presence of arabinoglactans, mannans, and pectins	470:522	The monosaccharide composition had a complex mixture, suggesting the presence of arabinoglactans, mannans, and pectins.
28115086	5	45	theme	blood	687:691	arg1	monocytes					693:701	blood monocytes	687:701	blood monocytes	687:701	Treatment with 60mg/kg SFP reduced blood monocytes and neutrophils, reduced the tumor activity of N-acetylglucosaminidase, myeloperoxidase, and nitric oxide, increased blood lymphocytes, and increased the levels of tumor necrosis factor α (TNF-α) in tumor tissue.
28115086	0	46	from	wine	99:102	arg1	rats					132:135	Walker-256 tumor-bearing rats	107:135	Walker-256 tumor-bearing rats	107:135	Necroptosis mediates the antineoplastic effects of the soluble fraction of polysaccharide from red wine in Walker-256 tumor-bearing rats.
28115086	0	46	from	wine	99:102	arg1	effects					40:46	the antineoplastic effects	21:46	the antineoplastic effects of the soluble fraction of polysaccharide from red wine in Walker-256 tumor-bearing rats	21:135	Necroptosis mediates the antineoplastic effects of the soluble fraction of polysaccharide from red wine in Walker-256 tumor-bearing rats.
28115086	5	47	theme	tumor	902:906	arg1	tissue					908:913	tumor tissue	902:913	tumor tissue	902:913	Treatment with 60mg/kg SFP reduced blood monocytes and neutrophils, reduced the tumor activity of N-acetylglucosaminidase, myeloperoxidase, and nitric oxide, increased blood lymphocytes, and increased the levels of tumor necrosis factor α (TNF-α) in tumor tissue.
28115086	5	48	theme	factor	882:887	arg1	levels					857:862	the levels	853:862	the levels of tumor necrosis factor α (TNF-α)	853:897	Treatment with 60mg/kg SFP reduced blood monocytes and neutrophils, reduced the tumor activity of N-acetylglucosaminidase, myeloperoxidase, and nitric oxide, increased blood lymphocytes, and increased the levels of tumor necrosis factor α (TNF-α) in tumor tissue.
28115086	7	49	theme	necroptosis	1209:1219	arg1	pathway					1221:1227	the necroptosis pathway	1205:1227	the necroptosis pathway	1205:1227	The antineoplastic effect of SFP appears to be attributable to its action on the immune system by controlling the tumor microenvironment and stimulating TNF-α production, which may trigger the necroptosis pathway.
28115086	0	50	theme	fraction	63:70	arg1	effects					40:46	the antineoplastic effects	21:46	the antineoplastic effects of the soluble fraction of polysaccharide from red wine in Walker-256 tumor-bearing rats	21:135	Necroptosis mediates the antineoplastic effects of the soluble fraction of polysaccharide from red wine in Walker-256 tumor-bearing rats.
28115086	4	51	dep	30	545:546	arg1	oral					561:564	oral	561:564	oral	561:564	Treatment with SFP (30 and 60mg/kg, oral) for 14days significantly reduced the tumor weight and volume compared with controls.
28115086	2	52	theme	antitumor	262:270	arg1	activity					272:279	the antitumor activity	258:279	the antitumor activity of the soluble fraction of polysaccharides (SFP), extracted from cabernet franc red wine, in Walker-256 tumor-bearing rats	258:402	The present study investigated the antitumor activity of the soluble fraction of polysaccharides (SFP), extracted from cabernet franc red wine, in Walker-256 tumor-bearing rats.
28115086	4	53	theme	tumor	604:608	arg1	weight					610:615	the tumor weight	600:615	the tumor weight	600:615	Treatment with SFP (30 and 60mg/kg, oral) for 14days significantly reduced the tumor weight and volume compared with controls.
28678201	5	0	theme	HSQC	797:800	arg1	spectroscopy					807:818	2D heteronuclear single quantum coherence (HSQC) NMR spectroscopy	754:818	2D heteronuclear single quantum coherence (HSQC) NMR spectroscopy	754:818	To integrate the analytical profile, 2D heteronuclear single quantum coherence (HSQC) NMR spectroscopy was applied and found effective to identify and quantify GAG component signals as well as those of some process signatures of danaparoid active pharmaceutical ingredient (API) batches.
28678201	3	1	theme	enzymatic	523:531	arg1	depolymerization					533:548	enzymatic depolymerization	523:548	enzymatic depolymerization	523:548	Currently dermatan sulfate and chondroitin sulfate are quantified by UV detection of their unsaturated disaccharides obtained by enzymatic depolymerization.
28678201	5	2	theme	quantum	778:784	arg1	spectroscopy					807:818	2D heteronuclear single quantum coherence (HSQC) NMR spectroscopy	754:818	2D heteronuclear single quantum coherence (HSQC) NMR spectroscopy	754:818	To integrate the analytical profile, 2D heteronuclear single quantum coherence (HSQC) NMR spectroscopy was applied and found effective to identify and quantify GAG component signals as well as those of some process signatures of danaparoid active pharmaceutical ingredient (API) batches.
28678201	6	3	dep	exclusion	1184:1192	arg1	HP-SEC-TDA					1233:1242	HP-SEC-TDA	1233:1242	HP-SEC-TDA	1233:1242	Analyses of components of both API samples and size separated fractions proceeded through the determination and distribution of the molecular weight (Mw) by high performance size exclusion chromatographic triple detector array (HP-SEC-TDA), chain mapping by LC/MS, and mono- (¹H and 13C) and bi-dimensional (HSQC) NMR spectroscopy.
28678201	6	3	dep	exclusion	1184:1192	arg1	¹H					1281:1282	¹H	1281:1282	¹H	1281:1282	Analyses of components of both API samples and size separated fractions proceeded through the determination and distribution of the molecular weight (Mw) by high performance size exclusion chromatographic triple detector array (HP-SEC-TDA), chain mapping by LC/MS, and mono- (¹H and 13C) and bi-dimensional (HSQC) NMR spectroscopy.
28678201	6	3	dep	exclusion	1184:1192	arg1	array					1226:1230	chromatographic triple detector array	1194:1230	high performance size exclusion chromatographic triple detector array (HP-SEC-TDA)	1162:1243	Analyses of components of both API samples and size separated fractions proceeded through the determination and distribution of the molecular weight (Mw) by high performance size exclusion chromatographic triple detector array (HP-SEC-TDA), chain mapping by LC/MS, and mono- (¹H and 13C) and bi-dimensional (HSQC) NMR spectroscopy.
28678201	6	3	dep	exclusion	1184:1192	arg1	13C					1288:1290	13C	1288:1290	13C	1288:1290	Analyses of components of both API samples and size separated fractions proceeded through the determination and distribution of the molecular weight (Mw) by high performance size exclusion chromatographic triple detector array (HP-SEC-TDA), chain mapping by LC/MS, and mono- (¹H and 13C) and bi-dimensional (HSQC) NMR spectroscopy.
28678201	5	4	theme	analytical	734:743	arg1	profile					745:751	the analytical profile	730:751	the analytical profile	730:751	To integrate the analytical profile, 2D heteronuclear single quantum coherence (HSQC) NMR spectroscopy was applied and found effective to identify and quantify GAG component signals as well as those of some process signatures of danaparoid active pharmaceutical ingredient (API) batches.
28678201	2	5	theme	chondroitin	352:362	arg1	sulfate					364:370	chondroitin sulfate	352:370	chondroitin sulfate	352:370	Heparan sulfate is the major component, dermatan sulfate and chondroitin sulfate being the minor ones.
28678201	2	5	theme	chondroitin	352:362	arg1	component					320:328	the major component	310:328	the major component	310:328	Heparan sulfate is the major component, dermatan sulfate and chondroitin sulfate being the minor ones.
28678201	1	6	theme	porcine	255:261	arg1	extracts					281:288	porcine intestinal mucosa extracts	255:288	porcine intestinal mucosa extracts	255:288	Danaparoid sodium salt, is the active component of ORGARAN, an anticoagulant and antithrombotic drug constituted of three glycosaminoglycans (GAGs) obtained from porcine intestinal mucosa extracts.
28678201	5	7	theme	2D	754:755	arg1	spectroscopy					807:818	2D heteronuclear single quantum coherence (HSQC) NMR spectroscopy	754:818	2D heteronuclear single quantum coherence (HSQC) NMR spectroscopy	754:818	To integrate the analytical profile, 2D heteronuclear single quantum coherence (HSQC) NMR spectroscopy was applied and found effective to identify and quantify GAG component signals as well as those of some process signatures of danaparoid active pharmaceutical ingredient (API) batches.
28678201	7	8	theme	large	1346:1350	arg1	isolation					1374:1382	large scale chromatographic isolation	1346:1382	large scale chromatographic isolation	1346:1382	Finally, large scale chromatographic isolation and depolymerization of each GAG followed by LC/MS and 2D-NMR analysis, allowed the sequences to be defined and components to be evaluated of each GAG including oxidized residues of hexosamines and uronic acids at the reducing ends.
28678201	7	9	theme	scale	1352:1356	arg1	isolation					1374:1382	large scale chromatographic isolation	1346:1382	large scale chromatographic isolation	1346:1382	Finally, large scale chromatographic isolation and depolymerization of each GAG followed by LC/MS and 2D-NMR analysis, allowed the sequences to be defined and components to be evaluated of each GAG including oxidized residues of hexosamines and uronic acids at the reducing ends.
28678201	6	10	theme	detector	1217:1224	arg1	HP-SEC-TDA					1233:1242	HP-SEC-TDA	1233:1242	HP-SEC-TDA	1233:1242	Analyses of components of both API samples and size separated fractions proceeded through the determination and distribution of the molecular weight (Mw) by high performance size exclusion chromatographic triple detector array (HP-SEC-TDA), chain mapping by LC/MS, and mono- (¹H and 13C) and bi-dimensional (HSQC) NMR spectroscopy.
28678201	6	10	theme	detector	1217:1224	arg1	array					1226:1230	chromatographic triple detector array	1194:1230	high performance size exclusion chromatographic triple detector array (HP-SEC-TDA)	1162:1243	Analyses of components of both API samples and size separated fractions proceeded through the determination and distribution of the molecular weight (Mw) by high performance size exclusion chromatographic triple detector array (HP-SEC-TDA), chain mapping by LC/MS, and mono- (¹H and 13C) and bi-dimensional (HSQC) NMR spectroscopy.
28678201	4	11	theme	advanced	689:696	arg1	tools					698:702	more advanced tools	684:702	more advanced tools	684:702	Due to the complexity of danaparoid biopolymers and the presence of shared components, an orthogonal approach has been applied using more advanced tools and methods.
28678201	1	12	theme	intestinal	263:272	arg1	extracts					281:288	porcine intestinal mucosa extracts	255:288	porcine intestinal mucosa extracts	255:288	Danaparoid sodium salt, is the active component of ORGARAN, an anticoagulant and antithrombotic drug constituted of three glycosaminoglycans (GAGs) obtained from porcine intestinal mucosa extracts.
28678201	5	13	theme	active	957:962	arg1	ingredient					979:988	active pharmaceutical ingredient	957:988	danaparoid active pharmaceutical ingredient (API) batches	946:1002	To integrate the analytical profile, 2D heteronuclear single quantum coherence (HSQC) NMR spectroscopy was applied and found effective to identify and quantify GAG component signals as well as those of some process signatures of danaparoid active pharmaceutical ingredient (API) batches.
28678201	5	13	theme	active	957:962	arg1	API					991:993	API	991:993	API	991:993	To integrate the analytical profile, 2D heteronuclear single quantum coherence (HSQC) NMR spectroscopy was applied and found effective to identify and quantify GAG component signals as well as those of some process signatures of danaparoid active pharmaceutical ingredient (API) batches.
28678201	6	14	theme	triple	1210:1215	arg1	HP-SEC-TDA					1233:1242	HP-SEC-TDA	1233:1242	HP-SEC-TDA	1233:1242	Analyses of components of both API samples and size separated fractions proceeded through the determination and distribution of the molecular weight (Mw) by high performance size exclusion chromatographic triple detector array (HP-SEC-TDA), chain mapping by LC/MS, and mono- (¹H and 13C) and bi-dimensional (HSQC) NMR spectroscopy.
28678201	6	14	theme	triple	1210:1215	arg1	array					1226:1230	chromatographic triple detector array	1194:1230	high performance size exclusion chromatographic triple detector array (HP-SEC-TDA)	1162:1243	Analyses of components of both API samples and size separated fractions proceeded through the determination and distribution of the molecular weight (Mw) by high performance size exclusion chromatographic triple detector array (HP-SEC-TDA), chain mapping by LC/MS, and mono- (¹H and 13C) and bi-dimensional (HSQC) NMR spectroscopy.
28678201	2	15	theme	Heparan	291:297	arg1	sulfate					299:305	Heparan sulfate	291:305	Heparan sulfate	291:305	Heparan sulfate is the major component, dermatan sulfate and chondroitin sulfate being the minor ones.
28678201	2	15	theme	Heparan	291:297	arg1	component					320:328	the major component	310:328	the major component	310:328	Heparan sulfate is the major component, dermatan sulfate and chondroitin sulfate being the minor ones.
28678201	1	16	theme	mucosa	274:279	arg1	extracts					281:288	porcine intestinal mucosa extracts	255:288	porcine intestinal mucosa extracts	255:288	Danaparoid sodium salt, is the active component of ORGARAN, an anticoagulant and antithrombotic drug constituted of three glycosaminoglycans (GAGs) obtained from porcine intestinal mucosa extracts.
28678201	5	17	theme	single	771:776	arg1	spectroscopy					807:818	2D heteronuclear single quantum coherence (HSQC) NMR spectroscopy	754:818	2D heteronuclear single quantum coherence (HSQC) NMR spectroscopy	754:818	To integrate the analytical profile, 2D heteronuclear single quantum coherence (HSQC) NMR spectroscopy was applied and found effective to identify and quantify GAG component signals as well as those of some process signatures of danaparoid active pharmaceutical ingredient (API) batches.
28678201	6	18	theme	fractions	1067:1075	arg1	components					1017:1026	components	1017:1026	components of both API samples and size separated fractions	1017:1075	Analyses of components of both API samples and size separated fractions proceeded through the determination and distribution of the molecular weight (Mw) by high performance size exclusion chromatographic triple detector array (HP-SEC-TDA), chain mapping by LC/MS, and mono- (¹H and 13C) and bi-dimensional (HSQC) NMR spectroscopy.
28678201	5	19	theme	batches	996:1002	arg1	signatures					932:941	some process signatures	919:941	some process signatures of danaparoid active pharmaceutical ingredient (API) batches	919:1002	To integrate the analytical profile, 2D heteronuclear single quantum coherence (HSQC) NMR spectroscopy was applied and found effective to identify and quantify GAG component signals as well as those of some process signatures of danaparoid active pharmaceutical ingredient (API) batches.
28678201	1	20	theme	anticoagulant	156:168	arg1	ORGARAN					144:150	ORGARAN	144:150	ORGARAN	144:150	Danaparoid sodium salt, is the active component of ORGARAN, an anticoagulant and antithrombotic drug constituted of three glycosaminoglycans (GAGs) obtained from porcine intestinal mucosa extracts.
28678201	1	20	theme	anticoagulant	156:168	arg1	drug					189:192	an anticoagulant and antithrombotic drug	153:192	an anticoagulant and antithrombotic drug constituted of three glycosaminoglycans (GAGs) obtained from porcine intestinal mucosa extracts	153:288	Danaparoid sodium salt, is the active component of ORGARAN, an anticoagulant and antithrombotic drug constituted of three glycosaminoglycans (GAGs) obtained from porcine intestinal mucosa extracts.
28678201	4	21	theme	shared	619:624	arg1	components					626:635	shared components	619:635	shared components	619:635	Due to the complexity of danaparoid biopolymers and the presence of shared components, an orthogonal approach has been applied using more advanced tools and methods.
28678201	7	22	theme	2D-NMR	1439:1444	arg1	analysis					1446:1453	LC/MS and 2D-NMR analysis	1429:1453	analysis	1446:1453	Finally, large scale chromatographic isolation and depolymerization of each GAG followed by LC/MS and 2D-NMR analysis, allowed the sequences to be defined and components to be evaluated of each GAG including oxidized residues of hexosamines and uronic acids at the reducing ends.
28678201	5	23	theme	NMR	803:805	arg1	spectroscopy					807:818	2D heteronuclear single quantum coherence (HSQC) NMR spectroscopy	754:818	2D heteronuclear single quantum coherence (HSQC) NMR spectroscopy	754:818	To integrate the analytical profile, 2D heteronuclear single quantum coherence (HSQC) NMR spectroscopy was applied and found effective to identify and quantify GAG component signals as well as those of some process signatures of danaparoid active pharmaceutical ingredient (API) batches.
28678201	7	24	theme	oxidized	1545:1552	arg1	hexosamines					1566:1576	hexosamines	1566:1576	hexosamines	1566:1576	Finally, large scale chromatographic isolation and depolymerization of each GAG followed by LC/MS and 2D-NMR analysis, allowed the sequences to be defined and components to be evaluated of each GAG including oxidized residues of hexosamines and uronic acids at the reducing ends.
28678201	7	24	theme	oxidized	1545:1552	arg1	acids					1589:1593	uronic acids	1582:1593	uronic acids	1582:1593	Finally, large scale chromatographic isolation and depolymerization of each GAG followed by LC/MS and 2D-NMR analysis, allowed the sequences to be defined and components to be evaluated of each GAG including oxidized residues of hexosamines and uronic acids at the reducing ends.
28678201	7	24	theme	oxidized	1545:1552	arg1	residues					1554:1561	oxidized residues	1545:1561	oxidized residues of hexosamines and uronic acids	1545:1593	Finally, large scale chromatographic isolation and depolymerization of each GAG followed by LC/MS and 2D-NMR analysis, allowed the sequences to be defined and components to be evaluated of each GAG including oxidized residues of hexosamines and uronic acids at the reducing ends.
28678201	6	25	theme	samples	1040:1046	arg1	components					1017:1026	components	1017:1026	components of both API samples and size separated fractions	1017:1075	Analyses of components of both API samples and size separated fractions proceeded through the determination and distribution of the molecular weight (Mw) by high performance size exclusion chromatographic triple detector array (HP-SEC-TDA), chain mapping by LC/MS, and mono- (¹H and 13C) and bi-dimensional (HSQC) NMR spectroscopy.
28678201	6	26	theme	size	1179:1182	arg1	exclusion					1184:1192	high performance size exclusion chromatographic triple detector array (HP-SEC-TDA)	1162:1243	high performance size exclusion chromatographic triple detector array (HP-SEC-TDA)	1162:1243	Analyses of components of both API samples and size separated fractions proceeded through the determination and distribution of the molecular weight (Mw) by high performance size exclusion chromatographic triple detector array (HP-SEC-TDA), chain mapping by LC/MS, and mono- (¹H and 13C) and bi-dimensional (HSQC) NMR spectroscopy.
28678201	5	27	theme	coherence	786:794	arg1	spectroscopy					807:818	2D heteronuclear single quantum coherence (HSQC) NMR spectroscopy	754:818	2D heteronuclear single quantum coherence (HSQC) NMR spectroscopy	754:818	To integrate the analytical profile, 2D heteronuclear single quantum coherence (HSQC) NMR spectroscopy was applied and found effective to identify and quantify GAG component signals as well as those of some process signatures of danaparoid active pharmaceutical ingredient (API) batches.
28678201	1	28	theme	Danaparoid	93:102	arg1	component					131:139	the active component	120:139	the active component of ORGARAN, an anticoagulant and antithrombotic drug constituted of three glycosaminoglycans (GAGs) obtained from porcine intestinal mucosa extracts	120:288	Danaparoid sodium salt, is the active component of ORGARAN, an anticoagulant and antithrombotic drug constituted of three glycosaminoglycans (GAGs) obtained from porcine intestinal mucosa extracts.
28678201	1	28	theme	Danaparoid	93:102	arg1	salt					111:114	Danaparoid sodium salt,	93:115	salt	111:114	Danaparoid sodium salt, is the active component of ORGARAN, an anticoagulant and antithrombotic drug constituted of three glycosaminoglycans (GAGs) obtained from porcine intestinal mucosa extracts.
28678201	6	29	theme	bi-dimensional	1297:1310	arg1	spectroscopy					1323:1334	bi-dimensional (HSQC) NMR spectroscopy	1297:1334	bi-dimensional (HSQC) NMR spectroscopy	1297:1334	Analyses of components of both API samples and size separated fractions proceeded through the determination and distribution of the molecular weight (Mw) by high performance size exclusion chromatographic triple detector array (HP-SEC-TDA), chain mapping by LC/MS, and mono- (¹H and 13C) and bi-dimensional (HSQC) NMR spectroscopy.
28678201	1	30	theme	antithrombotic	174:187	arg1	ORGARAN					144:150	ORGARAN	144:150	ORGARAN	144:150	Danaparoid sodium salt, is the active component of ORGARAN, an anticoagulant and antithrombotic drug constituted of three glycosaminoglycans (GAGs) obtained from porcine intestinal mucosa extracts.
28678201	1	30	theme	antithrombotic	174:187	arg1	drug					189:192	an anticoagulant and antithrombotic drug	153:192	an anticoagulant and antithrombotic drug constituted of three glycosaminoglycans (GAGs) obtained from porcine intestinal mucosa extracts	153:288	Danaparoid sodium salt, is the active component of ORGARAN, an anticoagulant and antithrombotic drug constituted of three glycosaminoglycans (GAGs) obtained from porcine intestinal mucosa extracts.
28678201	4	31	theme	biopolymers	587:597	arg1	complexity					562:571	the complexity	558:571	the complexity of danaparoid biopolymers	558:597	Due to the complexity of danaparoid biopolymers and the presence of shared components, an orthogonal approach has been applied using more advanced tools and methods.
28678201	4	31	theme	biopolymers	587:597	arg1	presence					607:614	the presence	603:614	the presence of shared components	603:635	Due to the complexity of danaparoid biopolymers and the presence of shared components, an orthogonal approach has been applied using more advanced tools and methods.
28678201	5	32	theme	component	881:889	arg1	signals					891:897	GAG component signals	877:897	GAG component signals as well as those of some process signatures of danaparoid active pharmaceutical ingredient (API) batches	877:1002	To integrate the analytical profile, 2D heteronuclear single quantum coherence (HSQC) NMR spectroscopy was applied and found effective to identify and quantify GAG component signals as well as those of some process signatures of danaparoid active pharmaceutical ingredient (API) batches.
28678201	2	33	theme	major	314:318	arg1	sulfate					340:346	dermatan sulfate	331:346	dermatan sulfate	331:346	Heparan sulfate is the major component, dermatan sulfate and chondroitin sulfate being the minor ones.
28678201	2	33	theme	major	314:318	arg1	sulfate					364:370	chondroitin sulfate	352:370	chondroitin sulfate	352:370	Heparan sulfate is the major component, dermatan sulfate and chondroitin sulfate being the minor ones.
28678201	2	33	theme	major	314:318	arg1	sulfate					299:305	Heparan sulfate	291:305	Heparan sulfate	291:305	Heparan sulfate is the major component, dermatan sulfate and chondroitin sulfate being the minor ones.
28678201	2	33	theme	major	314:318	arg1	component					320:328	the major component	310:328	the major component	310:328	Heparan sulfate is the major component, dermatan sulfate and chondroitin sulfate being the minor ones.
28678201	6	34	theme	NMR	1319:1321	arg1	spectroscopy					1323:1334	bi-dimensional (HSQC) NMR spectroscopy	1297:1334	bi-dimensional (HSQC) NMR spectroscopy	1297:1334	Analyses of components of both API samples and size separated fractions proceeded through the determination and distribution of the molecular weight (Mw) by high performance size exclusion chromatographic triple detector array (HP-SEC-TDA), chain mapping by LC/MS, and mono- (¹H and 13C) and bi-dimensional (HSQC) NMR spectroscopy.
28678201	2	35	theme	minor	382:386	arg1	ones					388:391	the minor ones	378:391	the minor ones	378:391	Heparan sulfate is the major component, dermatan sulfate and chondroitin sulfate being the minor ones.
28678201	0	36	theme	Complex	31:37	arg1	Drug					50:53	Danaparoid Complex Extractive Drug	20:53	Danaparoid Complex Extractive Drug	20:53	Characterization of Danaparoid Complex Extractive Drug by an Orthogonal Analytical Approach.
28678201	4	37	theme	danaparoid	576:585	arg1	biopolymers					587:597	danaparoid biopolymers	576:597	danaparoid biopolymers	576:597	Due to the complexity of danaparoid biopolymers and the presence of shared components, an orthogonal approach has been applied using more advanced tools and methods.
28678201	5	38	theme	danaparoid	946:955	arg1	batches					996:1002	danaparoid active pharmaceutical ingredient (API) batches	946:1002	danaparoid active pharmaceutical ingredient (API) batches	946:1002	To integrate the analytical profile, 2D heteronuclear single quantum coherence (HSQC) NMR spectroscopy was applied and found effective to identify and quantify GAG component signals as well as those of some process signatures of danaparoid active pharmaceutical ingredient (API) batches.
28678201	5	39	theme	ingredient	979:988	arg1	batches					996:1002	danaparoid active pharmaceutical ingredient (API) batches	946:1002	danaparoid active pharmaceutical ingredient (API) batches	946:1002	To integrate the analytical profile, 2D heteronuclear single quantum coherence (HSQC) NMR spectroscopy was applied and found effective to identify and quantify GAG component signals as well as those of some process signatures of danaparoid active pharmaceutical ingredient (API) batches.
28678201	1	40	theme	sodium	104:109	arg1	component					131:139	the active component	120:139	the active component of ORGARAN, an anticoagulant and antithrombotic drug constituted of three glycosaminoglycans (GAGs) obtained from porcine intestinal mucosa extracts	120:288	Danaparoid sodium salt, is the active component of ORGARAN, an anticoagulant and antithrombotic drug constituted of three glycosaminoglycans (GAGs) obtained from porcine intestinal mucosa extracts.
28678201	1	40	theme	sodium	104:109	arg1	salt					111:114	Danaparoid sodium salt,	93:115	salt	111:114	Danaparoid sodium salt, is the active component of ORGARAN, an anticoagulant and antithrombotic drug constituted of three glycosaminoglycans (GAGs) obtained from porcine intestinal mucosa extracts.
28678201	5	41	theme	pharmaceutical	964:977	arg1	ingredient					979:988	active pharmaceutical ingredient	957:988	danaparoid active pharmaceutical ingredient (API) batches	946:1002	To integrate the analytical profile, 2D heteronuclear single quantum coherence (HSQC) NMR spectroscopy was applied and found effective to identify and quantify GAG component signals as well as those of some process signatures of danaparoid active pharmaceutical ingredient (API) batches.
28678201	5	41	theme	pharmaceutical	964:977	arg1	API					991:993	API	991:993	API	991:993	To integrate the analytical profile, 2D heteronuclear single quantum coherence (HSQC) NMR spectroscopy was applied and found effective to identify and quantify GAG component signals as well as those of some process signatures of danaparoid active pharmaceutical ingredient (API) batches.
28678201	6	42	dep	determination	1099:1111	arg1	the					1095:1097	the	1095:1097	the	1095:1097	Analyses of components of both API samples and size separated fractions proceeded through the determination and distribution of the molecular weight (Mw) by high performance size exclusion chromatographic triple detector array (HP-SEC-TDA), chain mapping by LC/MS, and mono- (¹H and 13C) and bi-dimensional (HSQC) NMR spectroscopy.
28678201	6	43	theme	performance	1167:1177	arg1	exclusion					1184:1192	high performance size exclusion chromatographic triple detector array (HP-SEC-TDA)	1162:1243	high performance size exclusion chromatographic triple detector array (HP-SEC-TDA)	1162:1243	Analyses of components of both API samples and size separated fractions proceeded through the determination and distribution of the molecular weight (Mw) by high performance size exclusion chromatographic triple detector array (HP-SEC-TDA), chain mapping by LC/MS, and mono- (¹H and 13C) and bi-dimensional (HSQC) NMR spectroscopy.
28678201	0	44	theme	Drug	50:53	arg1	Characterization					0:15	Characterization	0:15	Characterization of Danaparoid Complex Extractive Drug by an Orthogonal Analytical Approach.	0:91	Characterization of Danaparoid Complex Extractive Drug by an Orthogonal Analytical Approach.
28678201	6	45	theme	separated	1057:1065	arg1	fractions					1067:1075	size separated fractions	1052:1075	size separated fractions	1052:1075	Analyses of components of both API samples and size separated fractions proceeded through the determination and distribution of the molecular weight (Mw) by high performance size exclusion chromatographic triple detector array (HP-SEC-TDA), chain mapping by LC/MS, and mono- (¹H and 13C) and bi-dimensional (HSQC) NMR spectroscopy.
28678201	3	46	theme	UV	463:464	arg1	detection					466:474	UV detection	463:474	UV detection of their unsaturated disaccharides obtained by enzymatic depolymerization	463:548	Currently dermatan sulfate and chondroitin sulfate are quantified by UV detection of their unsaturated disaccharides obtained by enzymatic depolymerization.
28678201	0	47	theme	Extractive	39:48	arg1	Drug					50:53	Danaparoid Complex Extractive Drug	20:53	Danaparoid Complex Extractive Drug	20:53	Characterization of Danaparoid Complex Extractive Drug by an Orthogonal Analytical Approach.
28678201	3	48	theme	chondroitin	425:435	arg1	sulfate					437:443	chondroitin sulfate	425:443	chondroitin sulfate	425:443	Currently dermatan sulfate and chondroitin sulfate are quantified by UV detection of their unsaturated disaccharides obtained by enzymatic depolymerization.
28678201	5	49	theme	heteronuclear	757:769	arg1	spectroscopy					807:818	2D heteronuclear single quantum coherence (HSQC) NMR spectroscopy	754:818	2D heteronuclear single quantum coherence (HSQC) NMR spectroscopy	754:818	To integrate the analytical profile, 2D heteronuclear single quantum coherence (HSQC) NMR spectroscopy was applied and found effective to identify and quantify GAG component signals as well as those of some process signatures of danaparoid active pharmaceutical ingredient (API) batches.
28678201	7	50	theme	chromatographic	1358:1372	arg1	isolation					1374:1382	large scale chromatographic isolation	1346:1382	large scale chromatographic isolation	1346:1382	Finally, large scale chromatographic isolation and depolymerization of each GAG followed by LC/MS and 2D-NMR analysis, allowed the sequences to be defined and components to be evaluated of each GAG including oxidized residues of hexosamines and uronic acids at the reducing ends.
28678201	5	51	theme	GAG	877:879	arg1	signals					891:897	GAG component signals	877:897	GAG component signals as well as those of some process signatures of danaparoid active pharmaceutical ingredient (API) batches	877:1002	To integrate the analytical profile, 2D heteronuclear single quantum coherence (HSQC) NMR spectroscopy was applied and found effective to identify and quantify GAG component signals as well as those of some process signatures of danaparoid active pharmaceutical ingredient (API) batches.
28678201	3	52	theme	dermatan	404:411	arg1	sulfate					413:419	dermatan sulfate	404:419	dermatan sulfate	404:419	Currently dermatan sulfate and chondroitin sulfate are quantified by UV detection of their unsaturated disaccharides obtained by enzymatic depolymerization.
28678201	7	53	theme	reducing	1602:1609	arg1	ends					1611:1614	the reducing ends	1598:1614	the reducing ends	1598:1614	Finally, large scale chromatographic isolation and depolymerization of each GAG followed by LC/MS and 2D-NMR analysis, allowed the sequences to be defined and components to be evaluated of each GAG including oxidized residues of hexosamines and uronic acids at the reducing ends.
28678201	7	54	theme	uronic	1582:1587	arg1	acids					1589:1593	uronic acids	1582:1593	uronic acids	1582:1593	Finally, large scale chromatographic isolation and depolymerization of each GAG followed by LC/MS and 2D-NMR analysis, allowed the sequences to be defined and components to be evaluated of each GAG including oxidized residues of hexosamines and uronic acids at the reducing ends.
28678201	6	55	theme	chain	1246:1250	arg1	mapping					1252:1258	chain mapping	1246:1258	chain mapping by LC/MS	1246:1267	Analyses of components of both API samples and size separated fractions proceeded through the determination and distribution of the molecular weight (Mw) by high performance size exclusion chromatographic triple detector array (HP-SEC-TDA), chain mapping by LC/MS, and mono- (¹H and 13C) and bi-dimensional (HSQC) NMR spectroscopy.
28678201	5	56	theme	process	924:930	arg1	signatures					932:941	some process signatures	919:941	some process signatures of danaparoid active pharmaceutical ingredient (API) batches	919:1002	To integrate the analytical profile, 2D heteronuclear single quantum coherence (HSQC) NMR spectroscopy was applied and found effective to identify and quantify GAG component signals as well as those of some process signatures of danaparoid active pharmaceutical ingredient (API) batches.
28678201	6	57	theme	HSQC	1313:1316	arg1	spectroscopy					1323:1334	bi-dimensional (HSQC) NMR spectroscopy	1297:1334	bi-dimensional (HSQC) NMR spectroscopy	1297:1334	Analyses of components of both API samples and size separated fractions proceeded through the determination and distribution of the molecular weight (Mw) by high performance size exclusion chromatographic triple detector array (HP-SEC-TDA), chain mapping by LC/MS, and mono- (¹H and 13C) and bi-dimensional (HSQC) NMR spectroscopy.
28678201	7	58	theme	acids	1589:1593	arg1	hexosamines					1566:1576	hexosamines	1566:1576	hexosamines	1566:1576	Finally, large scale chromatographic isolation and depolymerization of each GAG followed by LC/MS and 2D-NMR analysis, allowed the sequences to be defined and components to be evaluated of each GAG including oxidized residues of hexosamines and uronic acids at the reducing ends.
28678201	7	58	theme	acids	1589:1593	arg1	acids					1589:1593	uronic acids	1582:1593	uronic acids	1582:1593	Finally, large scale chromatographic isolation and depolymerization of each GAG followed by LC/MS and 2D-NMR analysis, allowed the sequences to be defined and components to be evaluated of each GAG including oxidized residues of hexosamines and uronic acids at the reducing ends.
28678201	7	58	theme	acids	1589:1593	arg1	residues					1554:1561	oxidized residues	1545:1561	oxidized residues of hexosamines and uronic acids	1545:1593	Finally, large scale chromatographic isolation and depolymerization of each GAG followed by LC/MS and 2D-NMR analysis, allowed the sequences to be defined and components to be evaluated of each GAG including oxidized residues of hexosamines and uronic acids at the reducing ends.
28678201	3	59	theme	unsaturated	485:495	arg1	disaccharides					497:509	their unsaturated disaccharides	479:509	their unsaturated disaccharides obtained by enzymatic depolymerization	479:548	Currently dermatan sulfate and chondroitin sulfate are quantified by UV detection of their unsaturated disaccharides obtained by enzymatic depolymerization.
28678201	4	60	theme	components	626:635	arg1	complexity					562:571	the complexity	558:571	the complexity of danaparoid biopolymers	558:597	Due to the complexity of danaparoid biopolymers and the presence of shared components, an orthogonal approach has been applied using more advanced tools and methods.
28678201	4	60	theme	components	626:635	arg1	presence					607:614	the presence	603:614	the presence of shared components	603:635	Due to the complexity of danaparoid biopolymers and the presence of shared components, an orthogonal approach has been applied using more advanced tools and methods.
28678201	6	61	theme	high	1162:1165	arg1	exclusion					1184:1192	high performance size exclusion chromatographic triple detector array (HP-SEC-TDA)	1162:1243	high performance size exclusion chromatographic triple detector array (HP-SEC-TDA)	1162:1243	Analyses of components of both API samples and size separated fractions proceeded through the determination and distribution of the molecular weight (Mw) by high performance size exclusion chromatographic triple detector array (HP-SEC-TDA), chain mapping by LC/MS, and mono- (¹H and 13C) and bi-dimensional (HSQC) NMR spectroscopy.
28678201	7	62	dep	sequences	1468:1476	arg1	defined					1484:1490	defined	1484:1490	to be defined	1478:1490	Finally, large scale chromatographic isolation and depolymerization of each GAG followed by LC/MS and 2D-NMR analysis, allowed the sequences to be defined and components to be evaluated of each GAG including oxidized residues of hexosamines and uronic acids at the reducing ends.
28678201	6	63	theme	chromatographic	1194:1208	arg1	HP-SEC-TDA					1233:1242	HP-SEC-TDA	1233:1242	HP-SEC-TDA	1233:1242	Analyses of components of both API samples and size separated fractions proceeded through the determination and distribution of the molecular weight (Mw) by high performance size exclusion chromatographic triple detector array (HP-SEC-TDA), chain mapping by LC/MS, and mono- (¹H and 13C) and bi-dimensional (HSQC) NMR spectroscopy.
28678201	6	63	theme	chromatographic	1194:1208	arg1	array					1226:1230	chromatographic triple detector array	1194:1230	high performance size exclusion chromatographic triple detector array (HP-SEC-TDA)	1162:1243	Analyses of components of both API samples and size separated fractions proceeded through the determination and distribution of the molecular weight (Mw) by high performance size exclusion chromatographic triple detector array (HP-SEC-TDA), chain mapping by LC/MS, and mono- (¹H and 13C) and bi-dimensional (HSQC) NMR spectroscopy.
28678201	1	64	theme	active	124:129	arg1	component					131:139	the active component	120:139	the active component of ORGARAN, an anticoagulant and antithrombotic drug constituted of three glycosaminoglycans (GAGs) obtained from porcine intestinal mucosa extracts	120:288	Danaparoid sodium salt, is the active component of ORGARAN, an anticoagulant and antithrombotic drug constituted of three glycosaminoglycans (GAGs) obtained from porcine intestinal mucosa extracts.
28678201	1	64	theme	active	124:129	arg1	salt					111:114	Danaparoid sodium salt,	93:115	salt	111:114	Danaparoid sodium salt, is the active component of ORGARAN, an anticoagulant and antithrombotic drug constituted of three glycosaminoglycans (GAGs) obtained from porcine intestinal mucosa extracts.
28678201	6	65	theme	weight	1147:1152	arg1	distribution					1117:1128	distribution	1117:1128	distribution	1117:1128	Analyses of components of both API samples and size separated fractions proceeded through the determination and distribution of the molecular weight (Mw) by high performance size exclusion chromatographic triple detector array (HP-SEC-TDA), chain mapping by LC/MS, and mono- (¹H and 13C) and bi-dimensional (HSQC) NMR spectroscopy.
28678201	6	65	theme	weight	1147:1152	arg1	determination					1099:1111	determination	1099:1111	determination	1099:1111	Analyses of components of both API samples and size separated fractions proceeded through the determination and distribution of the molecular weight (Mw) by high performance size exclusion chromatographic triple detector array (HP-SEC-TDA), chain mapping by LC/MS, and mono- (¹H and 13C) and bi-dimensional (HSQC) NMR spectroscopy.
28678201	7	66	theme	GAG	1413:1415	arg1	depolymerization					1388:1403	depolymerization	1388:1403	depolymerization	1388:1403	Finally, large scale chromatographic isolation and depolymerization of each GAG followed by LC/MS and 2D-NMR analysis, allowed the sequences to be defined and components to be evaluated of each GAG including oxidized residues of hexosamines and uronic acids at the reducing ends.
28678201	7	66	theme	GAG	1413:1415	arg1	isolation					1374:1382	large scale chromatographic isolation	1346:1382	large scale chromatographic isolation	1346:1382	Finally, large scale chromatographic isolation and depolymerization of each GAG followed by LC/MS and 2D-NMR analysis, allowed the sequences to be defined and components to be evaluated of each GAG including oxidized residues of hexosamines and uronic acids at the reducing ends.
28678201	7	67	theme	LC/MS	1429:1433	arg1	analysis					1446:1453	LC/MS and 2D-NMR analysis	1429:1453	analysis	1446:1453	Finally, large scale chromatographic isolation and depolymerization of each GAG followed by LC/MS and 2D-NMR analysis, allowed the sequences to be defined and components to be evaluated of each GAG including oxidized residues of hexosamines and uronic acids at the reducing ends.
28678201	3	68	theme	disaccharides	497:509	arg1	detection					466:474	UV detection	463:474	UV detection of their unsaturated disaccharides obtained by enzymatic depolymerization	463:548	Currently dermatan sulfate and chondroitin sulfate are quantified by UV detection of their unsaturated disaccharides obtained by enzymatic depolymerization.
28678201	2	69	theme	dermatan	331:338	arg1	sulfate					340:346	dermatan sulfate	331:346	dermatan sulfate	331:346	Heparan sulfate is the major component, dermatan sulfate and chondroitin sulfate being the minor ones.
28678201	2	69	theme	dermatan	331:338	arg1	component					320:328	the major component	310:328	the major component	310:328	Heparan sulfate is the major component, dermatan sulfate and chondroitin sulfate being the minor ones.
28678201	0	70	theme	Analytical	72:81	arg1	Approach					83:90	an Orthogonal Analytical Approach	58:90	an Orthogonal Analytical Approach	58:90	Characterization of Danaparoid Complex Extractive Drug by an Orthogonal Analytical Approach.
28678201	6	71	theme	size	1052:1055	arg1	fractions					1067:1075	size separated fractions	1052:1075	size separated fractions	1052:1075	Analyses of components of both API samples and size separated fractions proceeded through the determination and distribution of the molecular weight (Mw) by high performance size exclusion chromatographic triple detector array (HP-SEC-TDA), chain mapping by LC/MS, and mono- (¹H and 13C) and bi-dimensional (HSQC) NMR spectroscopy.
28678201	7	72	theme	hexosamines	1566:1576	arg1	hexosamines					1566:1576	hexosamines	1566:1576	hexosamines	1566:1576	Finally, large scale chromatographic isolation and depolymerization of each GAG followed by LC/MS and 2D-NMR analysis, allowed the sequences to be defined and components to be evaluated of each GAG including oxidized residues of hexosamines and uronic acids at the reducing ends.
28678201	7	72	theme	hexosamines	1566:1576	arg1	acids					1589:1593	uronic acids	1582:1593	uronic acids	1582:1593	Finally, large scale chromatographic isolation and depolymerization of each GAG followed by LC/MS and 2D-NMR analysis, allowed the sequences to be defined and components to be evaluated of each GAG including oxidized residues of hexosamines and uronic acids at the reducing ends.
28678201	7	72	theme	hexosamines	1566:1576	arg1	residues					1554:1561	oxidized residues	1545:1561	oxidized residues of hexosamines and uronic acids	1545:1593	Finally, large scale chromatographic isolation and depolymerization of each GAG followed by LC/MS and 2D-NMR analysis, allowed the sequences to be defined and components to be evaluated of each GAG including oxidized residues of hexosamines and uronic acids at the reducing ends.
28678201	6	73	theme	molecular	1137:1145	arg1	Mw					1155:1156	Mw	1155:1156	Mw	1155:1156	Analyses of components of both API samples and size separated fractions proceeded through the determination and distribution of the molecular weight (Mw) by high performance size exclusion chromatographic triple detector array (HP-SEC-TDA), chain mapping by LC/MS, and mono- (¹H and 13C) and bi-dimensional (HSQC) NMR spectroscopy.
28678201	6	73	theme	molecular	1137:1145	arg1	weight					1147:1152	the molecular weight	1133:1152	the molecular weight (Mw) by high performance size exclusion chromatographic triple detector array (HP-SEC-TDA), chain mapping by LC/MS, and mono- (¹H and 13C) and bi-dimensional (HSQC) NMR spectroscopy	1133:1334	Analyses of components of both API samples and size separated fractions proceeded through the determination and distribution of the molecular weight (Mw) by high performance size exclusion chromatographic triple detector array (HP-SEC-TDA), chain mapping by LC/MS, and mono- (¹H and 13C) and bi-dimensional (HSQC) NMR spectroscopy.
28678201	6	74	theme	components	1017:1026	arg1	Analyses					1005:1012	Analyses	1005:1012	Analyses of components of both API samples and size separated fractions	1005:1075	Analyses of components of both API samples and size separated fractions proceeded through the determination and distribution of the molecular weight (Mw) by high performance size exclusion chromatographic triple detector array (HP-SEC-TDA), chain mapping by LC/MS, and mono- (¹H and 13C) and bi-dimensional (HSQC) NMR spectroscopy.
28678201	6	75	theme	API	1036:1038	arg1	samples					1040:1046	API samples	1036:1046	API samples	1036:1046	Analyses of components of both API samples and size separated fractions proceeded through the determination and distribution of the molecular weight (Mw) by high performance size exclusion chromatographic triple detector array (HP-SEC-TDA), chain mapping by LC/MS, and mono- (¹H and 13C) and bi-dimensional (HSQC) NMR spectroscopy.
28678201	0	76	theme	Orthogonal	61:70	arg1	Approach					83:90	an Orthogonal Analytical Approach	58:90	an Orthogonal Analytical Approach	58:90	Characterization of Danaparoid Complex Extractive Drug by an Orthogonal Analytical Approach.
28678201	0	77	theme	Danaparoid	20:29	arg1	Drug					50:53	Danaparoid Complex Extractive Drug	20:53	Danaparoid Complex Extractive Drug	20:53	Characterization of Danaparoid Complex Extractive Drug by an Orthogonal Analytical Approach.
28678201	1	78	theme	ORGARAN	144:150	arg1	component					131:139	the active component	120:139	the active component of ORGARAN, an anticoagulant and antithrombotic drug constituted of three glycosaminoglycans (GAGs) obtained from porcine intestinal mucosa extracts	120:288	Danaparoid sodium salt, is the active component of ORGARAN, an anticoagulant and antithrombotic drug constituted of three glycosaminoglycans (GAGs) obtained from porcine intestinal mucosa extracts.
28678201	1	78	theme	ORGARAN	144:150	arg1	salt					111:114	Danaparoid sodium salt,	93:115	salt	111:114	Danaparoid sodium salt, is the active component of ORGARAN, an anticoagulant and antithrombotic drug constituted of three glycosaminoglycans (GAGs) obtained from porcine intestinal mucosa extracts.
28678201	4	79	theme	orthogonal	641:650	arg1	approach					652:659	an orthogonal approach	638:659	an orthogonal approach	638:659	Due to the complexity of danaparoid biopolymers and the presence of shared components, an orthogonal approach has been applied using more advanced tools and methods.
29292140	5	0	theme	new	728:730	arg1	peaks					743:747	two new absorption peaks	724:747	two new absorption peaks at 941cm-1 and 1048cm-1	724:771	In an FT-IR analysis, in comparison to POP, two new absorption peaks at 941cm-1 and 1048cm-1 were observed due to COSe and SeO bonds.
29292140	7	1	theme	-induced	1014:1021	arg1	stress					1033:1038	hydrogen peroxide (H2O2)-induced oxidative stress	990:1038	hydrogen peroxide (H2O2)-induced oxidative stress	990:1038	Compared to POP, Se-POP had a superior ability to reduce hydrogen peroxide (H2O2)-induced oxidative stress and apoptosis in murine skeletal muscle (C2C12) cells.
29292140	10	2	theme	functional	1397:1406	arg1	foods					1408:1412	functional foods	1397:1412	functional foods	1397:1412	These results suggest that Se-POP is a candidate dietary supplement for functional foods.
29292140	5	3	theme	absorption	732:741	arg1	peaks					743:747	two new absorption peaks	724:747	two new absorption peaks at 941cm-1 and 1048cm-1	724:771	In an FT-IR analysis, in comparison to POP, two new absorption peaks at 941cm-1 and 1048cm-1 were observed due to COSe and SeO bonds.
29292140	7	4	from	apoptosis	1044:1052	arg1	cells					1088:1092	murine skeletal muscle (C2C12) cells	1057:1092	murine skeletal muscle (C2C12) cells	1057:1092	Compared to POP, Se-POP had a superior ability to reduce hydrogen peroxide (H2O2)-induced oxidative stress and apoptosis in murine skeletal muscle (C2C12) cells.
29292140	7	5	theme	oxidative	1023:1031	arg1	stress					1033:1038	hydrogen peroxide (H2O2)-induced oxidative stress	990:1038	hydrogen peroxide (H2O2)-induced oxidative stress	990:1038	Compared to POP, Se-POP had a superior ability to reduce hydrogen peroxide (H2O2)-induced oxidative stress and apoptosis in murine skeletal muscle (C2C12) cells.
29292140	6	6	theme	hydroxyl	838:845	arg1	study					823:827	vitro study	817:827	vitro study of DPPH, hydroxyl, and ABTS free radicals	817:869	In vitro study of DPPH, hydroxyl, and ABTS free radicals, Se-POP had a stronger antioxidant capacity than that of POP.
29292140	3	7	contain	had	442:444	arg2	0.94×104Da					496:505	0.94×104Da	496:505	0.94×104Da	496:505	Se-POP and POP had similar average molecular weights (0.95×104Da and 0.94×104Da, respectively).
29292140	3	7	contain	had	442:444	arg1	POP					438:440	POP	438:440	POP	438:440	Se-POP and POP had similar average molecular weights (0.95×104Da and 0.94×104Da, respectively).
29292140	3	7	contain	had	442:444	arg2	0.95×104Da					481:490	0.95×104Da	481:490	0.95×104Da	481:490	Se-POP and POP had similar average molecular weights (0.95×104Da and 0.94×104Da, respectively).
29292140	3	7	contain	had	442:444	arg2	weights					472:478	similar average molecular weights	446:478	similar average molecular weights (0.95×104Da and 0.94×104Da, respectively)	446:520	Se-POP and POP had similar average molecular weights (0.95×104Da and 0.94×104Da, respectively).
29292140	3	7	contain	had	442:444	arg1	Se-POP					427:432	Se-POP	427:432	Se-POP	427:432	Se-POP and POP had similar average molecular weights (0.95×104Da and 0.94×104Da, respectively).
29292140	8	8	from	selenium	1107:1114	arg1	Se-POP					1119:1124	Se-POP	1119:1124	Se-POP	1119:1124	In general, selenium in Se-POP contributed to high antioxidant and biological activities.
29292140	2	9	from	bodies	378:383	arg1	extraction					347:356	hot water extraction	337:356	hot water extraction from fresh fruiting bodies of P. ostreatus with or without selenium	337:424	Selenium-enriched Pleurotus ostreatus polysaccharide (Se-POP) and the native polysaccharide (POP) were obtained by hot water extraction from fresh fruiting bodies of P. ostreatus with or without selenium.
29292140	9	10	theme	free	1300:1303	arg1	radicals					1305:1312	free radicals	1300:1312	free radicals in humans	1300:1322	Se-POP acts as a potent antioxidant and has the ability to prevent oxidation via reactive oxygen species (ROS) and free radicals in humans.
29292140	7	11	theme	C2C12	1081:1085	arg1	cells					1088:1092	murine skeletal muscle (C2C12) cells	1057:1092	murine skeletal muscle (C2C12) cells	1057:1092	Compared to POP, Se-POP had a superior ability to reduce hydrogen peroxide (H2O2)-induced oxidative stress and apoptosis in murine skeletal muscle (C2C12) cells.
29292140	6	12	theme	DPPH	832:835	arg1	hydroxyl					838:845	DPPH, hydroxyl, and ABTS free radicals	832:869	hydroxyl	838:845	In vitro study of DPPH, hydroxyl, and ABTS free radicals, Se-POP had a stronger antioxidant capacity than that of POP.
29292140	7	13	theme	skeletal	1064:1071	arg1	cells					1088:1092	murine skeletal muscle (C2C12) cells	1057:1092	murine skeletal muscle (C2C12) cells	1057:1092	Compared to POP, Se-POP had a superior ability to reduce hydrogen peroxide (H2O2)-induced oxidative stress and apoptosis in murine skeletal muscle (C2C12) cells.
29292140	5	14	located	observed	778:785	arg2	peaks					743:747	two new absorption peaks	724:747	two new absorption peaks at 941cm-1 and 1048cm-1	724:771	In an FT-IR analysis, in comparison to POP, two new absorption peaks at 941cm-1 and 1048cm-1 were observed due to COSe and SeO bonds.
29292140	5	14	located	observed	778:785	arg1	analysis					692:699	an FT-IR analysis	683:699	an FT-IR analysis	683:699	In an FT-IR analysis, in comparison to POP, two new absorption peaks at 941cm-1 and 1048cm-1 were observed due to COSe and SeO bonds.
29292140	5	14	located	observed	778:785	arg1	comparison					705:714	comparison	705:714	comparison to POP	705:721	In an FT-IR analysis, in comparison to POP, two new absorption peaks at 941cm-1 and 1048cm-1 were observed due to COSe and SeO bonds.
29292140	4	15	theme	different	656:664	arg1	ratios					672:677	different molar ratios	656:677	different molar ratios	656:677	Se-POP was mainly composed of fucose, rhamnose, arabinose, galactose, glucose, and xylose, similar to the composition of POP, but in different molar ratios.
29292140	1	16	theme	high	192:195	arg1	activities					210:219	their high anti-oxidant activities	186:219	their high anti-oxidant activities	186:219	Selenium-enriched polysaccharides have become a major research focus owing to their high anti-oxidant activities.
29292140	5	17	from	1048cm-1	764:771	arg1	peaks					743:747	two new absorption peaks	724:747	two new absorption peaks at 941cm-1 and 1048cm-1	724:771	In an FT-IR analysis, in comparison to POP, two new absorption peaks at 941cm-1 and 1048cm-1 were observed due to COSe and SeO bonds.
29292140	9	18	theme	oxygen	1275:1280	arg1	species					1282:1288	reactive oxygen species	1266:1288	reactive oxygen species (ROS)	1266:1294	Se-POP acts as a potent antioxidant and has the ability to prevent oxidation via reactive oxygen species (ROS) and free radicals in humans.
29292140	9	18	theme	oxygen	1275:1280	arg1	ROS					1291:1293	ROS	1291:1293	ROS	1291:1293	Se-POP acts as a potent antioxidant and has the ability to prevent oxidation via reactive oxygen species (ROS) and free radicals in humans.
29292140	7	19	contain	had	957:959	arg1	Se-POP					950:955	Se-POP	950:955	Se-POP	950:955	Compared to POP, Se-POP had a superior ability to reduce hydrogen peroxide (H2O2)-induced oxidative stress and apoptosis in murine skeletal muscle (C2C12) cells.
29292140	7	19	contain	had	957:959	arg2	ability					972:978	a superior ability	961:978	a superior ability to reduce hydrogen peroxide (H2O2)-induced oxidative stress and apoptosis in murine skeletal muscle (C2C12) cells	961:1092	Compared to POP, Se-POP had a superior ability to reduce hydrogen peroxide (H2O2)-induced oxidative stress and apoptosis in murine skeletal muscle (C2C12) cells.
29292140	1	20	theme	anti-oxidant	197:208	arg1	activities					210:219	their high anti-oxidant activities	186:219	their high anti-oxidant activities	186:219	Selenium-enriched polysaccharides have become a major research focus owing to their high anti-oxidant activities.
29292140	7	21	theme	muscle	1073:1078	arg1	cells					1088:1092	murine skeletal muscle (C2C12) cells	1057:1092	murine skeletal muscle (C2C12) cells	1057:1092	Compared to POP, Se-POP had a superior ability to reduce hydrogen peroxide (H2O2)-induced oxidative stress and apoptosis in murine skeletal muscle (C2C12) cells.
29292140	7	22	from	stress	1033:1038	arg1	cells					1088:1092	murine skeletal muscle (C2C12) cells	1057:1092	murine skeletal muscle (C2C12) cells	1057:1092	Compared to POP, Se-POP had a superior ability to reduce hydrogen peroxide (H2O2)-induced oxidative stress and apoptosis in murine skeletal muscle (C2C12) cells.
29292140	3	23	theme	similar	446:452	arg1	0.95×104Da					481:490	0.95×104Da	481:490	0.95×104Da	481:490	Se-POP and POP had similar average molecular weights (0.95×104Da and 0.94×104Da, respectively).
29292140	3	23	theme	similar	446:452	arg1	weights					472:478	similar average molecular weights	446:478	similar average molecular weights (0.95×104Da and 0.94×104Da, respectively)	446:520	Se-POP and POP had similar average molecular weights (0.95×104Da and 0.94×104Da, respectively).
29292140	3	23	theme	similar	446:452	arg1	0.94×104Da					496:505	0.94×104Da	496:505	0.94×104Da	496:505	Se-POP and POP had similar average molecular weights (0.95×104Da and 0.94×104Da, respectively).
29292140	6	24	theme	antioxidant	894:904	arg1	capacity					906:913	a stronger antioxidant capacity	883:913	a stronger antioxidant capacity	883:913	In vitro study of DPPH, hydroxyl, and ABTS free radicals, Se-POP had a stronger antioxidant capacity than that of POP.
29292140	7	25	theme	hydrogen	990:997	arg1	peroxide					999:1006	hydrogen peroxide	990:1006	hydrogen peroxide (H2O2)	990:1013	Compared to POP, Se-POP had a superior ability to reduce hydrogen peroxide (H2O2)-induced oxidative stress and apoptosis in murine skeletal muscle (C2C12) cells.
29292140	7	25	theme	hydrogen	990:997	arg1	H2O2					1009:1012	H2O2	1009:1012	H2O2	1009:1012	Compared to POP, Se-POP had a superior ability to reduce hydrogen peroxide (H2O2)-induced oxidative stress and apoptosis in murine skeletal muscle (C2C12) cells.
29292140	2	26	theme	water	341:345	arg1	extraction					347:356	hot water extraction	337:356	hot water extraction from fresh fruiting bodies of P. ostreatus with or without selenium	337:424	Selenium-enriched Pleurotus ostreatus polysaccharide (Se-POP) and the native polysaccharide (POP) were obtained by hot water extraction from fresh fruiting bodies of P. ostreatus with or without selenium.
29292140	3	27	theme	average	454:460	arg1	0.95×104Da					481:490	0.95×104Da	481:490	0.95×104Da	481:490	Se-POP and POP had similar average molecular weights (0.95×104Da and 0.94×104Da, respectively).
29292140	3	27	theme	average	454:460	arg1	weights					472:478	similar average molecular weights	446:478	similar average molecular weights (0.95×104Da and 0.94×104Da, respectively)	446:520	Se-POP and POP had similar average molecular weights (0.95×104Da and 0.94×104Da, respectively).
29292140	3	27	theme	average	454:460	arg1	0.94×104Da					496:505	0.94×104Da	496:505	0.94×104Da	496:505	Se-POP and POP had similar average molecular weights (0.95×104Da and 0.94×104Da, respectively).
29292140	0	28	theme	Pleurotus	32:40	arg1	polysaccharides					52:66	Se-enriched Pleurotus ostreatus polysaccharides	20:66	Se-enriched Pleurotus ostreatus polysaccharides	20:66	Characterization of Se-enriched Pleurotus ostreatus polysaccharides and their antioxidant effects in vitro.
29292140	6	29	theme	stronger	885:892	arg1	capacity					906:913	a stronger antioxidant capacity	883:913	a stronger antioxidant capacity	883:913	In vitro study of DPPH, hydroxyl, and ABTS free radicals, Se-POP had a stronger antioxidant capacity than that of POP.
29292140	10	30	theme	candidate	1364:1372	arg1	supplement					1382:1391	a candidate dietary supplement	1362:1391	a candidate dietary supplement for functional foods	1362:1412	These results suggest that Se-POP is a candidate dietary supplement for functional foods.
29292140	10	30	theme	candidate	1364:1372	arg1	Se-POP					1352:1357	Se-POP	1352:1357	Se-POP	1352:1357	These results suggest that Se-POP is a candidate dietary supplement for functional foods.
29292140	2	31	theme	hot	337:339	arg1	extraction					347:356	hot water extraction	337:356	hot water extraction from fresh fruiting bodies of P. ostreatus with or without selenium	337:424	Selenium-enriched Pleurotus ostreatus polysaccharide (Se-POP) and the native polysaccharide (POP) were obtained by hot water extraction from fresh fruiting bodies of P. ostreatus with or without selenium.
29292140	8	32	theme	antioxidant	1146:1156	arg1	activities					1173:1182	high antioxidant and biological activities	1141:1182	high antioxidant and biological activities	1141:1182	In general, selenium in Se-POP contributed to high antioxidant and biological activities.
29292140	1	33	theme	Selenium-enriched	108:124	arg1	polysaccharides					126:140	Selenium-enriched polysaccharides	108:140	Selenium-enriched polysaccharides	108:140	Selenium-enriched polysaccharides have become a major research focus owing to their high anti-oxidant activities.
29292140	5	34	theme	FT-IR	686:690	arg1	analysis					692:699	an FT-IR analysis	683:699	an FT-IR analysis	683:699	In an FT-IR analysis, in comparison to POP, two new absorption peaks at 941cm-1 and 1048cm-1 were observed due to COSe and SeO bonds.
29292140	6	35	theme	vitro	817:821	arg1	study					823:827	vitro study	817:827	vitro study of DPPH, hydroxyl, and ABTS free radicals	817:869	In vitro study of DPPH, hydroxyl, and ABTS free radicals, Se-POP had a stronger antioxidant capacity than that of POP.
29292140	8	36	theme	high	1141:1144	arg1	activities					1173:1182	high antioxidant and biological activities	1141:1182	high antioxidant and biological activities	1141:1182	In general, selenium in Se-POP contributed to high antioxidant and biological activities.
29292140	0	37	theme	polysaccharides	52:66	arg1	Characterization					0:15	Characterization	0:15	Characterization of Se-enriched Pleurotus ostreatus polysaccharides and their antioxidant effects in vitro.	0:106	Characterization of Se-enriched Pleurotus ostreatus polysaccharides and their antioxidant effects in vitro.
29292140	8	38	theme	biological	1162:1171	arg1	activities					1173:1182	high antioxidant and biological activities	1141:1182	high antioxidant and biological activities	1141:1182	In general, selenium in Se-POP contributed to high antioxidant and biological activities.
29292140	6	39	contain	had	879:881	arg2	capacity					906:913	a stronger antioxidant capacity	883:913	a stronger antioxidant capacity	883:913	In vitro study of DPPH, hydroxyl, and ABTS free radicals, Se-POP had a stronger antioxidant capacity than that of POP.
29292140	6	39	contain	had	879:881	arg1	Se-POP					872:877	Se-POP	872:877	Se-POP	872:877	In vitro study of DPPH, hydroxyl, and ABTS free radicals, Se-POP had a stronger antioxidant capacity than that of POP.
29292140	4	40	theme	POP	644:646	arg1	composition					629:639	the composition	625:639	the composition of POP	625:646	Se-POP was mainly composed of fucose, rhamnose, arabinose, galactose, glucose, and xylose, similar to the composition of POP, but in different molar ratios.
29292140	3	41	theme	molecular	462:470	arg1	0.95×104Da					481:490	0.95×104Da	481:490	0.95×104Da	481:490	Se-POP and POP had similar average molecular weights (0.95×104Da and 0.94×104Da, respectively).
29292140	3	41	theme	molecular	462:470	arg1	weights					472:478	similar average molecular weights	446:478	similar average molecular weights (0.95×104Da and 0.94×104Da, respectively)	446:520	Se-POP and POP had similar average molecular weights (0.95×104Da and 0.94×104Da, respectively).
29292140	3	41	theme	molecular	462:470	arg1	0.94×104Da					496:505	0.94×104Da	496:505	0.94×104Da	496:505	Se-POP and POP had similar average molecular weights (0.95×104Da and 0.94×104Da, respectively).
29292140	7	42	theme	superior	963:970	arg1	ability					972:978	a superior ability	961:978	a superior ability to reduce hydrogen peroxide (H2O2)-induced oxidative stress and apoptosis in murine skeletal muscle (C2C12) cells	961:1092	Compared to POP, Se-POP had a superior ability to reduce hydrogen peroxide (H2O2)-induced oxidative stress and apoptosis in murine skeletal muscle (C2C12) cells.
29292140	7	43	theme	murine	1057:1062	arg1	cells					1088:1092	murine skeletal muscle (C2C12) cells	1057:1092	murine skeletal muscle (C2C12) cells	1057:1092	Compared to POP, Se-POP had a superior ability to reduce hydrogen peroxide (H2O2)-induced oxidative stress and apoptosis in murine skeletal muscle (C2C12) cells.
29292140	5	44	theme	SeO	803:805	arg1	bonds					807:811	SeO bonds	803:811	SeO bonds	803:811	In an FT-IR analysis, in comparison to POP, two new absorption peaks at 941cm-1 and 1048cm-1 were observed due to COSe and SeO bonds.
29292140	9	45	from	species	1282:1288	arg1	humans					1317:1322	humans	1317:1322	humans	1317:1322	Se-POP acts as a potent antioxidant and has the ability to prevent oxidation via reactive oxygen species (ROS) and free radicals in humans.
29292140	9	46	theme	potent	1202:1207	arg1	Se-POP					1185:1190	Se-POP	1185:1190	Se-POP	1185:1190	Se-POP acts as a potent antioxidant and has the ability to prevent oxidation via reactive oxygen species (ROS) and free radicals in humans.
29292140	9	46	theme	potent	1202:1207	arg1	ability					1233:1239	the ability to prevent oxidation via reactive oxygen species (ROS) and free radicals in humans	1229:1322	the ability to prevent oxidation via reactive oxygen species (ROS) and free radicals in humans	1229:1322	Se-POP acts as a potent antioxidant and has the ability to prevent oxidation via reactive oxygen species (ROS) and free radicals in humans.
29292140	9	46	theme	potent	1202:1207	arg1	antioxidant					1209:1219	a potent antioxidant	1200:1219	a potent antioxidant	1200:1219	Se-POP acts as a potent antioxidant and has the ability to prevent oxidation via reactive oxygen species (ROS) and free radicals in humans.
29292140	6	47	theme	radicals	862:869	arg1	study					823:827	vitro study	817:827	vitro study of DPPH, hydroxyl, and ABTS free radicals	817:869	In vitro study of DPPH, hydroxyl, and ABTS free radicals, Se-POP had a stronger antioxidant capacity than that of POP.
29292140	6	48	theme	ABTS	852:855	arg1	radicals					862:869	DPPH, hydroxyl, and ABTS free radicals	832:869	radicals	862:869	In vitro study of DPPH, hydroxyl, and ABTS free radicals, Se-POP had a stronger antioxidant capacity than that of POP.
29292140	3	49	dep	weights	472:478	arg1	0.95×104Da					481:490	0.95×104Da	481:490	0.95×104Da	481:490	Se-POP and POP had similar average molecular weights (0.95×104Da and 0.94×104Da, respectively).
29292140	3	49	dep	weights	472:478	arg1	weights					472:478	similar average molecular weights	446:478	similar average molecular weights (0.95×104Da and 0.94×104Da, respectively)	446:520	Se-POP and POP had similar average molecular weights (0.95×104Da and 0.94×104Da, respectively).
29292140	3	49	dep	weights	472:478	arg1	0.94×104Da					496:505	0.94×104Da	496:505	0.94×104Da	496:505	Se-POP and POP had similar average molecular weights (0.95×104Da and 0.94×104Da, respectively).
29292140	9	50	contain	has	1225:1227	arg2	antioxidant					1209:1219	a potent antioxidant	1200:1219	a potent antioxidant	1200:1219	Se-POP acts as a potent antioxidant and has the ability to prevent oxidation via reactive oxygen species (ROS) and free radicals in humans.
29292140	9	50	contain	has	1225:1227	arg2	Se-POP					1185:1190	Se-POP	1185:1190	Se-POP	1185:1190	Se-POP acts as a potent antioxidant and has the ability to prevent oxidation via reactive oxygen species (ROS) and free radicals in humans.
29292140	9	50	contain	has	1225:1227	arg1	antioxidant					1209:1219	a potent antioxidant	1200:1219	a potent antioxidant	1200:1219	Se-POP acts as a potent antioxidant and has the ability to prevent oxidation via reactive oxygen species (ROS) and free radicals in humans.
29292140	9	50	contain	has	1225:1227	arg2	ability					1233:1239	the ability to prevent oxidation via reactive oxygen species (ROS) and free radicals in humans	1229:1322	the ability to prevent oxidation via reactive oxygen species (ROS) and free radicals in humans	1229:1322	Se-POP acts as a potent antioxidant and has the ability to prevent oxidation via reactive oxygen species (ROS) and free radicals in humans.
29292140	9	50	contain	has	1225:1227	arg1	Se-POP					1185:1190	Se-POP	1185:1190	Se-POP	1185:1190	Se-POP acts as a potent antioxidant and has the ability to prevent oxidation via reactive oxygen species (ROS) and free radicals in humans.
29292140	9	50	contain	has	1225:1227	arg1	ability					1233:1239	the ability to prevent oxidation via reactive oxygen species (ROS) and free radicals in humans	1229:1322	the ability to prevent oxidation via reactive oxygen species (ROS) and free radicals in humans	1229:1322	Se-POP acts as a potent antioxidant and has the ability to prevent oxidation via reactive oxygen species (ROS) and free radicals in humans.
29292140	2	51	theme	fruiting	369:376	arg1	bodies					378:383	fresh fruiting bodies	363:383	fresh fruiting bodies of P. ostreatus	363:399	Selenium-enriched Pleurotus ostreatus polysaccharide (Se-POP) and the native polysaccharide (POP) were obtained by hot water extraction from fresh fruiting bodies of P. ostreatus with or without selenium.
29292140	6	52	theme	free	857:860	arg1	radicals					862:869	DPPH, hydroxyl, and ABTS free radicals	832:869	radicals	862:869	In vitro study of DPPH, hydroxyl, and ABTS free radicals, Se-POP had a stronger antioxidant capacity than that of POP.
29292140	0	53	dep	Pleurotus	32:40	arg1	ostreatus					42:50	ostreatus	42:50	ostreatus	42:50	Characterization of Se-enriched Pleurotus ostreatus polysaccharides and their antioxidant effects in vitro.
29292140	4	54	theme	molar	666:670	arg1	ratios					672:677	different molar ratios	656:677	different molar ratios	656:677	Se-POP was mainly composed of fucose, rhamnose, arabinose, galactose, glucose, and xylose, similar to the composition of POP, but in different molar ratios.
29292140	1	55	theme	major	156:160	arg1	focus					171:175	a major research focus	154:175	a major research focus owing to their high anti-oxidant activities	154:219	Selenium-enriched polysaccharides have become a major research focus owing to their high anti-oxidant activities.
29292140	9	56	theme	reactive	1266:1273	arg1	species					1282:1288	reactive oxygen species	1266:1288	reactive oxygen species (ROS)	1266:1294	Se-POP acts as a potent antioxidant and has the ability to prevent oxidation via reactive oxygen species (ROS) and free radicals in humans.
29292140	9	56	theme	reactive	1266:1273	arg1	ROS					1291:1293	ROS	1291:1293	ROS	1291:1293	Se-POP acts as a potent antioxidant and has the ability to prevent oxidation via reactive oxygen species (ROS) and free radicals in humans.
29292140	2	57	dep	Pleurotus	240:248	arg1	ostreatus					250:258	ostreatus	250:258	ostreatus	250:258	Selenium-enriched Pleurotus ostreatus polysaccharide (Se-POP) and the native polysaccharide (POP) were obtained by hot water extraction from fresh fruiting bodies of P. ostreatus with or without selenium.
29292140	10	58	theme	dietary	1374:1380	arg1	supplement					1382:1391	a candidate dietary supplement	1362:1391	a candidate dietary supplement for functional foods	1362:1412	These results suggest that Se-POP is a candidate dietary supplement for functional foods.
29292140	10	58	theme	dietary	1374:1380	arg1	Se-POP					1352:1357	Se-POP	1352:1357	Se-POP	1352:1357	These results suggest that Se-POP is a candidate dietary supplement for functional foods.
29292140	2	59	theme	Pleurotus	240:248	arg1	Se-POP					276:281	Se-POP	276:281	Se-POP	276:281	Selenium-enriched Pleurotus ostreatus polysaccharide (Se-POP) and the native polysaccharide (POP) were obtained by hot water extraction from fresh fruiting bodies of P. ostreatus with or without selenium.
29292140	2	59	theme	Pleurotus	240:248	arg1	polysaccharide					260:273	Selenium-enriched Pleurotus ostreatus polysaccharide	222:273	Selenium-enriched Pleurotus ostreatus polysaccharide (Se-POP)	222:282	Selenium-enriched Pleurotus ostreatus polysaccharide (Se-POP) and the native polysaccharide (POP) were obtained by hot water extraction from fresh fruiting bodies of P. ostreatus with or without selenium.
29292140	1	60	theme	research	162:169	arg1	focus					171:175	a major research focus	154:175	a major research focus owing to their high anti-oxidant activities	154:219	Selenium-enriched polysaccharides have become a major research focus owing to their high anti-oxidant activities.
29292140	2	61	theme	fresh	363:367	arg1	bodies					378:383	fresh fruiting bodies	363:383	fresh fruiting bodies of P. ostreatus	363:399	Selenium-enriched Pleurotus ostreatus polysaccharide (Se-POP) and the native polysaccharide (POP) were obtained by hot water extraction from fresh fruiting bodies of P. ostreatus with or without selenium.
29292140	0	62	theme	effects	90:96	arg1	Characterization					0:15	Characterization	0:15	Characterization of Se-enriched Pleurotus ostreatus polysaccharides and their antioxidant effects in vitro.	0:106	Characterization of Se-enriched Pleurotus ostreatus polysaccharides and their antioxidant effects in vitro.
29292140	2	63	theme	Selenium-enriched	222:238	arg1	Se-POP					276:281	Se-POP	276:281	Se-POP	276:281	Selenium-enriched Pleurotus ostreatus polysaccharide (Se-POP) and the native polysaccharide (POP) were obtained by hot water extraction from fresh fruiting bodies of P. ostreatus with or without selenium.
29292140	2	63	theme	Selenium-enriched	222:238	arg1	polysaccharide					260:273	Selenium-enriched Pleurotus ostreatus polysaccharide	222:273	Selenium-enriched Pleurotus ostreatus polysaccharide (Se-POP)	222:282	Selenium-enriched Pleurotus ostreatus polysaccharide (Se-POP) and the native polysaccharide (POP) were obtained by hot water extraction from fresh fruiting bodies of P. ostreatus with or without selenium.
29292140	2	64	theme	native	292:297	arg1	POP					315:317	POP	315:317	POP	315:317	Selenium-enriched Pleurotus ostreatus polysaccharide (Se-POP) and the native polysaccharide (POP) were obtained by hot water extraction from fresh fruiting bodies of P. ostreatus with or without selenium.
29292140	2	64	theme	native	292:297	arg1	polysaccharide					299:312	the native polysaccharide	288:312	the native polysaccharide (POP)	288:318	Selenium-enriched Pleurotus ostreatus polysaccharide (Se-POP) and the native polysaccharide (POP) were obtained by hot water extraction from fresh fruiting bodies of P. ostreatus with or without selenium.
29292140	0	65	theme	antioxidant	78:88	arg1	effects					90:96	their antioxidant effects	72:96	their antioxidant effects in vitro	72:105	Characterization of Se-enriched Pleurotus ostreatus polysaccharides and their antioxidant effects in vitro.
29292140	5	66	from	941cm-1	752:758	arg1	peaks					743:747	two new absorption peaks	724:747	two new absorption peaks at 941cm-1 and 1048cm-1	724:771	In an FT-IR analysis, in comparison to POP, two new absorption peaks at 941cm-1 and 1048cm-1 were observed due to COSe and SeO bonds.
29292140	0	67	theme	Se-enriched	20:30	arg1	polysaccharides					52:66	Se-enriched Pleurotus ostreatus polysaccharides	20:66	Se-enriched Pleurotus ostreatus polysaccharides	20:66	Characterization of Se-enriched Pleurotus ostreatus polysaccharides and their antioxidant effects in vitro.
29292140	9	68	from	radicals	1305:1312	arg1	humans					1317:1322	humans	1317:1322	humans	1317:1322	Se-POP acts as a potent antioxidant and has the ability to prevent oxidation via reactive oxygen species (ROS) and free radicals in humans.
29292140	2	69	theme	ostreatus	391:399	arg1	bodies					378:383	fresh fruiting bodies	363:383	fresh fruiting bodies of P. ostreatus	363:399	Selenium-enriched Pleurotus ostreatus polysaccharide (Se-POP) and the native polysaccharide (POP) were obtained by hot water extraction from fresh fruiting bodies of P. ostreatus with or without selenium.
29292140	4	70	theme	similar	614:620	arg1	arabinose					571:579	arabinose	571:579	arabinose	571:579	Se-POP was mainly composed of fucose, rhamnose, arabinose, galactose, glucose, and xylose, similar to the composition of POP, but in different molar ratios.
29292140	4	70	theme	similar	614:620	arg1	xylose					606:611	xylose	606:611	xylose	606:611	Se-POP was mainly composed of fucose, rhamnose, arabinose, galactose, glucose, and xylose, similar to the composition of POP, but in different molar ratios.
29292140	4	70	theme	similar	614:620	arg1	galactose					582:590	galactose	582:590	galactose	582:590	Se-POP was mainly composed of fucose, rhamnose, arabinose, galactose, glucose, and xylose, similar to the composition of POP, but in different molar ratios.
29292140	4	70	theme	similar	614:620	arg1	fucose					553:558	fucose	553:558	fucose	553:558	Se-POP was mainly composed of fucose, rhamnose, arabinose, galactose, glucose, and xylose, similar to the composition of POP, but in different molar ratios.
29292140	4	70	theme	similar	614:620	arg1	rhamnose					561:568	rhamnose	561:568	rhamnose	561:568	Se-POP was mainly composed of fucose, rhamnose, arabinose, galactose, glucose, and xylose, similar to the composition of POP, but in different molar ratios.
29292140	4	70	theme	similar	614:620	arg1	glucose					593:599	glucose	593:599	glucose	593:599	Se-POP was mainly composed of fucose, rhamnose, arabinose, galactose, glucose, and xylose, similar to the composition of POP, but in different molar ratios.
27474628	0	0	theme	anticancer	101:110	arg1	systems					126:132	targeted anticancer drug delivery systems	92:132	targeted anticancer drug delivery systems	92:132	Lactobionic acid and carboxymethyl chitosan functionalized graphene oxide nanocomposites as targeted anticancer drug delivery systems.
27474628	6	1	theme	L929	911:914	arg1	line					921:924	the non-cancerous L929 cell line	893:924	the non-cancerous L929 cell line	893:924	Tests with shorter (2h) incubation times were undertaken to investigate the selectivity of the GO composites: under these conditions, neither DOX-loaded system was found to be toxic to the non-cancerous L929 cell line, but the LA-containing composite showed the ability to selectively induce cell death in cancerous (SMMC-7721) cells while the LA-free analogue was inactive here also.
27474628	6	2	theme	DOX-loaded	850:859	arg1	system					861:866	neither DOX-loaded system	842:866	neither DOX-loaded system	842:866	Tests with shorter (2h) incubation times were undertaken to investigate the selectivity of the GO composites: under these conditions, neither DOX-loaded system was found to be toxic to the non-cancerous L929 cell line, but the LA-containing composite showed the ability to selectively induce cell death in cancerous (SMMC-7721) cells while the LA-free analogue was inactive here also.
27474628	1	3	theme	carboxymethyl	241:253	arg1	chitosan					255:262	carboxymethyl chitosan	241:262	carboxymethyl chitosan (CMC)	241:268	In this work, we report a targeted drug delivery system built by functionalizing graphene oxide (GO) with carboxymethyl chitosan (CMC), fluorescein isothiocyanate and lactobionic acid (LA).
27474628	1	3	theme	carboxymethyl	241:253	arg1	isothiocyanate					283:296	fluorescein isothiocyanate	271:296	fluorescein isothiocyanate	271:296	In this work, we report a targeted drug delivery system built by functionalizing graphene oxide (GO) with carboxymethyl chitosan (CMC), fluorescein isothiocyanate and lactobionic acid (LA).
27474628	1	3	theme	carboxymethyl	241:253	arg1	acid					314:317	lactobionic acid	302:317	lactobionic acid (LA)	302:322	In this work, we report a targeted drug delivery system built by functionalizing graphene oxide (GO) with carboxymethyl chitosan (CMC), fluorescein isothiocyanate and lactobionic acid (LA).
27474628	1	3	theme	carboxymethyl	241:253	arg1	CMC					265:267	CMC	265:267	CMC	265:267	In this work, we report a targeted drug delivery system built by functionalizing graphene oxide (GO) with carboxymethyl chitosan (CMC), fluorescein isothiocyanate and lactobionic acid (LA).
27474628	0	4	theme	targeted	92:99	arg1	systems					126:132	targeted anticancer drug delivery systems	92:132	targeted anticancer drug delivery systems	92:132	Lactobionic acid and carboxymethyl chitosan functionalized graphene oxide nanocomposites as targeted anticancer drug delivery systems.
27474628	4	5	from	LA-functionalized	485:501	arg1	behavior					462:469	The release behavior	450:469	The release behavior from both the LA-functionalized and the LA-free material	450:526	The release behavior from both the LA-functionalized and the LA-free material is markedly pH sensitive.
27474628	4	5	from	LA-functionalized	485:501	arg1	sensitive					543:551	sensitive	543:551	sensitive	543:551	The release behavior from both the LA-functionalized and the LA-free material is markedly pH sensitive.
27474628	5	6	theme	high	576:579	arg1	biocompatibility					581:596	high biocompatibility	576:596	high biocompatibility	576:596	The modified GOs have high biocompatibility with the liver cancer cell line SMMC-7721, but can induce cell death after 24h incubation if loaded with DOX.
27474628	1	7	theme	targeted	161:168	arg1	system					184:189	a targeted drug delivery system	159:189	a targeted drug delivery system built by functionalizing graphene oxide (GO) with carboxymethyl chitosan (CMC), fluorescein isothiocyanate and lactobionic acid (LA)	159:322	In this work, we report a targeted drug delivery system built by functionalizing graphene oxide (GO) with carboxymethyl chitosan (CMC), fluorescein isothiocyanate and lactobionic acid (LA).
27474628	0	8	theme	delivery	117:124	arg1	systems					126:132	targeted anticancer drug delivery systems	92:132	targeted anticancer drug delivery systems	92:132	Lactobionic acid and carboxymethyl chitosan functionalized graphene oxide nanocomposites as targeted anticancer drug delivery systems.
27474628	0	9	theme	drug	112:115	arg1	systems					126:132	targeted anticancer drug delivery systems	92:132	targeted anticancer drug delivery systems	92:132	Lactobionic acid and carboxymethyl chitosan functionalized graphene oxide nanocomposites as targeted anticancer drug delivery systems.
27474628	4	10	theme	release	454:460	arg1	behavior					462:469	The release behavior	450:469	The release behavior from both the LA-functionalized and the LA-free material	450:526	The release behavior from both the LA-functionalized and the LA-free material is markedly pH sensitive.
27474628	4	10	theme	release	454:460	arg1	sensitive					543:551	sensitive	543:551	sensitive	543:551	The release behavior from both the LA-functionalized and the LA-free material is markedly pH sensitive.
27474628	7	11	theme	targeted	1180:1187	arg1	systems					1214:1220	targeted anticancer drug delivery systems	1180:1220	targeted anticancer drug delivery systems	1180:1220	These findings show that the modified GO materials are strong potential candidates for targeted anticancer drug delivery systems.
27474628	6	12	with	Tests	708:712	arg1	times					743:747	shorter (2h) incubation times	719:747	shorter (2h) incubation times	719:747	Tests with shorter (2h) incubation times were undertaken to investigate the selectivity of the GO composites: under these conditions, neither DOX-loaded system was found to be toxic to the non-cancerous L929 cell line, but the LA-containing composite showed the ability to selectively induce cell death in cancerous (SMMC-7721) cells while the LA-free analogue was inactive here also.
27474628	5	13	theme	liver	607:611	arg1	line					625:628	the liver cancer cell line SMMC-7721	603:638	the liver cancer cell line SMMC-7721	603:638	The modified GOs have high biocompatibility with the liver cancer cell line SMMC-7721, but can induce cell death after 24h incubation if loaded with DOX.
27474628	1	14	theme	drug	170:173	arg1	system					184:189	a targeted drug delivery system	159:189	a targeted drug delivery system built by functionalizing graphene oxide (GO) with carboxymethyl chitosan (CMC), fluorescein isothiocyanate and lactobionic acid (LA)	159:322	In this work, we report a targeted drug delivery system built by functionalizing graphene oxide (GO) with carboxymethyl chitosan (CMC), fluorescein isothiocyanate and lactobionic acid (LA).
27474628	7	15	theme	modified	1122:1129	arg1	materials					1134:1142	the modified GO materials	1118:1142	the modified GO materials	1118:1142	These findings show that the modified GO materials are strong potential candidates for targeted anticancer drug delivery systems.
27474628	7	15	theme	modified	1122:1129	arg1	candidates					1165:1174	strong potential candidates	1148:1174	strong potential candidates for targeted anticancer drug delivery systems	1148:1220	These findings show that the modified GO materials are strong potential candidates for targeted anticancer drug delivery systems.
27474628	5	16	theme	cancer	613:618	arg1	line					625:628	the liver cancer cell line SMMC-7721	603:638	the liver cancer cell line SMMC-7721	603:638	The modified GOs have high biocompatibility with the liver cancer cell line SMMC-7721, but can induce cell death after 24h incubation if loaded with DOX.
27474628	1	17	theme	delivery	175:182	arg1	system					184:189	a targeted drug delivery system	159:189	a targeted drug delivery system built by functionalizing graphene oxide (GO) with carboxymethyl chitosan (CMC), fluorescein isothiocyanate and lactobionic acid (LA)	159:322	In this work, we report a targeted drug delivery system built by functionalizing graphene oxide (GO) with carboxymethyl chitosan (CMC), fluorescein isothiocyanate and lactobionic acid (LA).
27474628	7	18	theme	strong	1148:1153	arg1	materials					1134:1142	the modified GO materials	1118:1142	the modified GO materials	1118:1142	These findings show that the modified GO materials are strong potential candidates for targeted anticancer drug delivery systems.
27474628	7	18	theme	strong	1148:1153	arg1	candidates					1165:1174	strong potential candidates	1148:1174	strong potential candidates for targeted anticancer drug delivery systems	1148:1220	These findings show that the modified GO materials are strong potential candidates for targeted anticancer drug delivery systems.
27474628	5	19	theme	cell	620:623	arg1	line					625:628	the liver cancer cell line SMMC-7721	603:638	the liver cancer cell line SMMC-7721	603:638	The modified GOs have high biocompatibility with the liver cancer cell line SMMC-7721, but can induce cell death after 24h incubation if loaded with DOX.
27474628	5	20	theme	modified	558:565	arg1	GOs					567:569	The modified GOs	554:569	The modified GOs	554:569	The modified GOs have high biocompatibility with the liver cancer cell line SMMC-7721, but can induce cell death after 24h incubation if loaded with DOX.
27474628	0	21	theme	Lactobionic	0:10	arg1	acid					12:15	Lactobionic acid	0:15	Lactobionic acid	0:15	Lactobionic acid and carboxymethyl chitosan functionalized graphene oxide nanocomposites as targeted anticancer drug delivery systems.
27474628	6	22	theme	incubation	732:741	arg1	times					743:747	shorter (2h) incubation times	719:747	shorter (2h) incubation times	719:747	Tests with shorter (2h) incubation times were undertaken to investigate the selectivity of the GO composites: under these conditions, neither DOX-loaded system was found to be toxic to the non-cancerous L929 cell line, but the LA-containing composite showed the ability to selectively induce cell death in cancerous (SMMC-7721) cells while the LA-free analogue was inactive here also.
27474628	6	23	theme	LA-containing	935:947	arg1	composite					949:957	the LA-containing composite	931:957	the LA-containing composite	931:957	Tests with shorter (2h) incubation times were undertaken to investigate the selectivity of the GO composites: under these conditions, neither DOX-loaded system was found to be toxic to the non-cancerous L929 cell line, but the LA-containing composite showed the ability to selectively induce cell death in cancerous (SMMC-7721) cells while the LA-free analogue was inactive here also.
27474628	0	24	theme	carboxymethyl	21:33	arg1	chitosan					35:42	carboxymethyl chitosan	21:42	carboxymethyl chitosan	21:42	Lactobionic acid and carboxymethyl chitosan functionalized graphene oxide nanocomposites as targeted anticancer drug delivery systems.
27474628	6	25	theme	cell	916:919	arg1	line					921:924	the non-cancerous L929 cell line	893:924	the non-cancerous L929 cell line	893:924	Tests with shorter (2h) incubation times were undertaken to investigate the selectivity of the GO composites: under these conditions, neither DOX-loaded system was found to be toxic to the non-cancerous L929 cell line, but the LA-containing composite showed the ability to selectively induce cell death in cancerous (SMMC-7721) cells while the LA-free analogue was inactive here also.
27474628	5	26	theme	24h	673:675	arg1	incubation					677:686	24h incubation	673:686	24h incubation	673:686	The modified GOs have high biocompatibility with the liver cancer cell line SMMC-7721, but can induce cell death after 24h incubation if loaded with DOX.
27474628	5	27	contain	have	571:574	arg1	GOs					567:569	The modified GOs	554:569	The modified GOs	554:569	The modified GOs have high biocompatibility with the liver cancer cell line SMMC-7721, but can induce cell death after 24h incubation if loaded with DOX.
27474628	5	27	contain	have	571:574	arg2	biocompatibility					581:596	high biocompatibility	576:596	high biocompatibility	576:596	The modified GOs have high biocompatibility with the liver cancer cell line SMMC-7721, but can induce cell death after 24h incubation if loaded with DOX.
27474628	1	28	theme	fluorescein	271:281	arg1	chitosan					255:262	carboxymethyl chitosan	241:262	carboxymethyl chitosan (CMC)	241:268	In this work, we report a targeted drug delivery system built by functionalizing graphene oxide (GO) with carboxymethyl chitosan (CMC), fluorescein isothiocyanate and lactobionic acid (LA).
27474628	1	28	theme	fluorescein	271:281	arg1	isothiocyanate					283:296	fluorescein isothiocyanate	271:296	fluorescein isothiocyanate	271:296	In this work, we report a targeted drug delivery system built by functionalizing graphene oxide (GO) with carboxymethyl chitosan (CMC), fluorescein isothiocyanate and lactobionic acid (LA).
27474628	6	29	dep	cancerous	1014:1022	arg1	SMMC-7721					1025:1033	SMMC-7721	1025:1033	SMMC-7721	1025:1033	Tests with shorter (2h) incubation times were undertaken to investigate the selectivity of the GO composites: under these conditions, neither DOX-loaded system was found to be toxic to the non-cancerous L929 cell line, but the LA-containing composite showed the ability to selectively induce cell death in cancerous (SMMC-7721) cells while the LA-free analogue was inactive here also.
27474628	0	30	theme	functionalized	44:57	arg1	oxide					68:72	functionalized graphene oxide	44:72	functionalized graphene oxide	44:72	Lactobionic acid and carboxymethyl chitosan functionalized graphene oxide nanocomposites as targeted anticancer drug delivery systems.
27474628	6	31	theme	non-cancerous	897:909	arg1	line					921:924	the non-cancerous L929 cell line	893:924	the non-cancerous L929 cell line	893:924	Tests with shorter (2h) incubation times were undertaken to investigate the selectivity of the GO composites: under these conditions, neither DOX-loaded system was found to be toxic to the non-cancerous L929 cell line, but the LA-containing composite showed the ability to selectively induce cell death in cancerous (SMMC-7721) cells while the LA-free analogue was inactive here also.
27474628	1	32	theme	graphene	216:223	arg1	oxide					225:229	graphene oxide	216:229	graphene oxide (GO)	216:234	In this work, we report a targeted drug delivery system built by functionalizing graphene oxide (GO) with carboxymethyl chitosan (CMC), fluorescein isothiocyanate and lactobionic acid (LA).
27474628	4	33	from	material	519:526	arg1	behavior					462:469	The release behavior	450:469	The release behavior from both the LA-functionalized and the LA-free material	450:526	The release behavior from both the LA-functionalized and the LA-free material is markedly pH sensitive.
27474628	4	33	from	material	519:526	arg1	sensitive					543:551	sensitive	543:551	sensitive	543:551	The release behavior from both the LA-functionalized and the LA-free material is markedly pH sensitive.
27474628	4	34	theme	LA-free	511:517	arg1	material					519:526	the LA-free material	507:526	the LA-free material	507:526	The release behavior from both the LA-functionalized and the LA-free material is markedly pH sensitive.
27474628	1	35	theme	lactobionic	302:312	arg1	chitosan					255:262	carboxymethyl chitosan	241:262	carboxymethyl chitosan (CMC)	241:268	In this work, we report a targeted drug delivery system built by functionalizing graphene oxide (GO) with carboxymethyl chitosan (CMC), fluorescein isothiocyanate and lactobionic acid (LA).
27474628	1	35	theme	lactobionic	302:312	arg1	LA					320:321	LA	320:321	LA	320:321	In this work, we report a targeted drug delivery system built by functionalizing graphene oxide (GO) with carboxymethyl chitosan (CMC), fluorescein isothiocyanate and lactobionic acid (LA).
27474628	1	35	theme	lactobionic	302:312	arg1	acid					314:317	lactobionic acid	302:317	lactobionic acid (LA)	302:322	In this work, we report a targeted drug delivery system built by functionalizing graphene oxide (GO) with carboxymethyl chitosan (CMC), fluorescein isothiocyanate and lactobionic acid (LA).
27474628	6	36	theme	composites	806:815	arg1	selectivity					784:794	the selectivity	780:794	the selectivity of the GO composites	780:815	Tests with shorter (2h) incubation times were undertaken to investigate the selectivity of the GO composites: under these conditions, neither DOX-loaded system was found to be toxic to the non-cancerous L929 cell line, but the LA-containing composite showed the ability to selectively induce cell death in cancerous (SMMC-7721) cells while the LA-free analogue was inactive here also.
27474628	2	37	theme	Analogous	325:333	arg1	controls					371:378	controls	371:378	controls	371:378	Analogous systems without LA were prepared as controls.
27474628	2	37	theme	Analogous	325:333	arg1	systems					335:341	Analogous systems	325:341	Analogous systems without LA	325:352	Analogous systems without LA were prepared as controls.
27474628	0	38	theme	graphene	59:66	arg1	oxide					68:72	functionalized graphene oxide	44:72	functionalized graphene oxide	44:72	Lactobionic acid and carboxymethyl chitosan functionalized graphene oxide nanocomposites as targeted anticancer drug delivery systems.
27474628	5	39	theme	cell	656:659	arg1	death					661:665	cell death	656:665	cell death	656:665	The modified GOs have high biocompatibility with the liver cancer cell line SMMC-7721, but can induce cell death after 24h incubation if loaded with DOX.
27474628	6	40	theme	shorter	719:725	arg1	times					743:747	shorter (2h) incubation times	719:747	shorter (2h) incubation times	719:747	Tests with shorter (2h) incubation times were undertaken to investigate the selectivity of the GO composites: under these conditions, neither DOX-loaded system was found to be toxic to the non-cancerous L929 cell line, but the LA-containing composite showed the ability to selectively induce cell death in cancerous (SMMC-7721) cells while the LA-free analogue was inactive here also.
27474628	6	41	theme	LA-free	1052:1058	arg1	inactive					1073:1080	inactive	1073:1080	inactive	1073:1080	Tests with shorter (2h) incubation times were undertaken to investigate the selectivity of the GO composites: under these conditions, neither DOX-loaded system was found to be toxic to the non-cancerous L929 cell line, but the LA-containing composite showed the ability to selectively induce cell death in cancerous (SMMC-7721) cells while the LA-free analogue was inactive here also.
27474628	6	41	theme	LA-free	1052:1058	arg1	analogue					1060:1067	the LA-free analogue	1048:1067	the LA-free analogue	1048:1067	Tests with shorter (2h) incubation times were undertaken to investigate the selectivity of the GO composites: under these conditions, neither DOX-loaded system was found to be toxic to the non-cancerous L929 cell line, but the LA-containing composite showed the ability to selectively induce cell death in cancerous (SMMC-7721) cells while the LA-free analogue was inactive here also.
27474628	7	42	dep	materials	1134:1142	arg1	GO					1131:1132	GO	1131:1132	GO	1131:1132	These findings show that the modified GO materials are strong potential candidates for targeted anticancer drug delivery systems.
27474628	1	43	dep	oxide	225:229	arg1	GO					232:233	GO	232:233	GO	232:233	In this work, we report a targeted drug delivery system built by functionalizing graphene oxide (GO) with carboxymethyl chitosan (CMC), fluorescein isothiocyanate and lactobionic acid (LA).
27474628	7	44	theme	delivery	1205:1212	arg1	systems					1214:1220	targeted anticancer drug delivery systems	1180:1220	targeted anticancer drug delivery systems	1180:1220	These findings show that the modified GO materials are strong potential candidates for targeted anticancer drug delivery systems.
27474628	7	45	theme	potential	1155:1163	arg1	materials					1134:1142	the modified GO materials	1118:1142	the modified GO materials	1118:1142	These findings show that the modified GO materials are strong potential candidates for targeted anticancer drug delivery systems.
27474628	7	45	theme	potential	1155:1163	arg1	candidates					1165:1174	strong potential candidates	1148:1174	strong potential candidates for targeted anticancer drug delivery systems	1148:1220	These findings show that the modified GO materials are strong potential candidates for targeted anticancer drug delivery systems.
27474628	0	46	dep	acid	12:15	arg1	oxide					68:72	functionalized graphene oxide	44:72	functionalized graphene oxide	44:72	Lactobionic acid and carboxymethyl chitosan functionalized graphene oxide nanocomposites as targeted anticancer drug delivery systems.
27474628	6	47	theme	cell	1000:1003	arg1	death					1005:1009	cell death	1000:1009	cell death	1000:1009	Tests with shorter (2h) incubation times were undertaken to investigate the selectivity of the GO composites: under these conditions, neither DOX-loaded system was found to be toxic to the non-cancerous L929 cell line, but the LA-containing composite showed the ability to selectively induce cell death in cancerous (SMMC-7721) cells while the LA-free analogue was inactive here also.
27474628	7	48	theme	anticancer	1189:1198	arg1	systems					1214:1220	targeted anticancer drug delivery systems	1180:1220	targeted anticancer drug delivery systems	1180:1220	These findings show that the modified GO materials are strong potential candidates for targeted anticancer drug delivery systems.
27474628	6	49	theme	cancerous	1014:1022	arg1	cells					1036:1040	cancerous (SMMC-7721) cells	1014:1040	cancerous (SMMC-7721) cells	1014:1040	Tests with shorter (2h) incubation times were undertaken to investigate the selectivity of the GO composites: under these conditions, neither DOX-loaded system was found to be toxic to the non-cancerous L929 cell line, but the LA-containing composite showed the ability to selectively induce cell death in cancerous (SMMC-7721) cells while the LA-free analogue was inactive here also.
27474628	7	50	theme	drug	1200:1203	arg1	systems					1214:1220	targeted anticancer drug delivery systems	1180:1220	targeted anticancer drug delivery systems	1180:1220	These findings show that the modified GO materials are strong potential candidates for targeted anticancer drug delivery systems.
27474628	6	51	dep	shorter	719:725	arg1	2h					728:729	2h	728:729	2h	728:729	Tests with shorter (2h) incubation times were undertaken to investigate the selectivity of the GO composites: under these conditions, neither DOX-loaded system was found to be toxic to the non-cancerous L929 cell line, but the LA-containing composite showed the ability to selectively induce cell death in cancerous (SMMC-7721) cells while the LA-free analogue was inactive here also.
27474628	6	52	dep	composites	806:815	arg1	GO					803:804	GO	803:804	GO	803:804	Tests with shorter (2h) incubation times were undertaken to investigate the selectivity of the GO composites: under these conditions, neither DOX-loaded system was found to be toxic to the non-cancerous L929 cell line, but the LA-containing composite showed the ability to selectively induce cell death in cancerous (SMMC-7721) cells while the LA-free analogue was inactive here also.
28774586	7	0	theme	XRD	1064:1066	arg1	microscopy					1099:1108	FTIR, XRD, optical and polarized optical microscopy	1058:1108	FTIR, XRD, optical and polarized optical microscopy	1058:1108	The cellulose/SCBP films without and with alkali treated SCBP were characterized by FTIR, XRD, optical and polarized optical microscopy, thermogravimetric analysis (TGA) and tensile tests.
28774586	3	1	theme	%	601:601	arg1	LiOH					603:606	8% LiOH	600:606	8% LiOH	600:606	Cellulose matrix was dissolved in the relatively safer aqueous solution mixture (8% LiOH and 15% Urea) precooled to -12.5°C. To the cellulose solution (SCBP) was added in 5-25wt% and the composite films were prepared by regeneration method using ethyl alcohol as a coagulant.
28774586	1	2	theme	bean	230:233	arg1	SCBP					243:246	SCBP	243:246	SCBP	243:246	As the annual production of the solid waste generable in the form of spent coffee bean powder (SCBP) is over 6 million tons, its utilization in the generation of green energy, waste water treatment and as a filler in biocomposites is desirable.
28774586	1	2	theme	bean	230:233	arg1	powder					235:240	spent coffee bean powder	217:240	spent coffee bean powder (SCBP)	217:247	As the annual production of the solid waste generable in the form of spent coffee bean powder (SCBP) is over 6 million tons, its utilization in the generation of green energy, waste water treatment and as a filler in biocomposites is desirable.
28774586	10	3	theme	treated	1574:1580	arg1	SCBP					1582:1585	alkali treated SCBP	1567:1585	alkali treated SCBP	1567:1585	Based on the improved tensile properties and photo resistivity, the cellulose/SCBP composite films with alkali treated SCBP may be considered for packaging and wrapping of flowers and vegetables.
28774586	3	4	theme	%	614:614	arg1	Urea					616:619	15% Urea	612:619	15% Urea	612:619	Cellulose matrix was dissolved in the relatively safer aqueous solution mixture (8% LiOH and 15% Urea) precooled to -12.5°C. To the cellulose solution (SCBP) was added in 5-25wt% and the composite films were prepared by regeneration method using ethyl alcohol as a coagulant.
28774586	9	5	used	used	1457:1460	arg2	SCBP					1448:1451	alkali treated SCBP	1433:1451	alkali treated SCBP	1433:1451	The thermal stability of the composite was higher at elevated temperatures when alkali treated SCBP was used.
28774586	0	6	theme	cellulose	103:111	arg1	matrix					113:118	cellulose matrix	103:118	cellulose matrix for packaging applications	103:145	Utilization of chemically treated municipal solid waste (spent coffee bean powder) as reinforcement in cellulose matrix for packaging applications.
28774586	1	7	theme	waste	186:190	arg1	generable					192:200	the solid waste generable	176:200	the solid waste generable	176:200	As the annual production of the solid waste generable in the form of spent coffee bean powder (SCBP) is over 6 million tons, its utilization in the generation of green energy, waste water treatment and as a filler in biocomposites is desirable.
28774586	2	8	theme	coffee	467:472	arg1	powder					479:484	coffee bean powder	467:484	coffee bean powder	467:484	The objective of this article is to analyze the possibilities to valorize coffee bean powder as a filler in cellulose matrix.
28774586	2	8	theme	coffee	467:472	arg1	filler					491:496	a filler	489:496	a filler in cellulose matrix	489:516	The objective of this article is to analyze the possibilities to valorize coffee bean powder as a filler in cellulose matrix.
28774586	3	9	dep	mixture	591:597	arg1	Urea					616:619	15% Urea	612:619	15% Urea	612:619	Cellulose matrix was dissolved in the relatively safer aqueous solution mixture (8% LiOH and 15% Urea) precooled to -12.5°C. To the cellulose solution (SCBP) was added in 5-25wt% and the composite films were prepared by regeneration method using ethyl alcohol as a coagulant.
28774586	3	9	dep	mixture	591:597	arg1	LiOH					603:606	8% LiOH	600:606	8% LiOH	600:606	Cellulose matrix was dissolved in the relatively safer aqueous solution mixture (8% LiOH and 15% Urea) precooled to -12.5°C. To the cellulose solution (SCBP) was added in 5-25wt% and the composite films were prepared by regeneration method using ethyl alcohol as a coagulant.
28774586	1	10	theme	water	330:334	arg1	treatment					336:344	waste water treatment	324:344	waste water treatment	324:344	As the annual production of the solid waste generable in the form of spent coffee bean powder (SCBP) is over 6 million tons, its utilization in the generation of green energy, waste water treatment and as a filler in biocomposites is desirable.
28774586	1	11	from	generation	296:305	arg1	biocomposites					365:377	biocomposites	365:377	biocomposites	365:377	As the annual production of the solid waste generable in the form of spent coffee bean powder (SCBP) is over 6 million tons, its utilization in the generation of green energy, waste water treatment and as a filler in biocomposites is desirable.
28774586	3	12	theme	cellulose	651:659	arg1	solution					661:668	the cellulose solution	647:668	the cellulose solution (SCBP)	647:675	Cellulose matrix was dissolved in the relatively safer aqueous solution mixture (8% LiOH and 15% Urea) precooled to -12.5°C. To the cellulose solution (SCBP) was added in 5-25wt% and the composite films were prepared by regeneration method using ethyl alcohol as a coagulant.
28774586	3	12	theme	cellulose	651:659	arg1	SCBP					671:674	SCBP	671:674	SCBP	671:674	Cellulose matrix was dissolved in the relatively safer aqueous solution mixture (8% LiOH and 15% Urea) precooled to -12.5°C. To the cellulose solution (SCBP) was added in 5-25wt% and the composite films were prepared by regeneration method using ethyl alcohol as a coagulant.
28774586	5	13	theme	alkali	883:888	arg1	SCBP					898:901	alkali treated SCBP	883:901	alkali treated SCBP	883:901	5% NaOH and the composite films were also prepared using alkali treated SCBP as a filler.
28774586	5	13	theme	alkali	883:888	arg1	filler					908:913	a filler	906:913	a filler	906:913	5% NaOH and the composite films were also prepared using alkali treated SCBP as a filler.
28774586	10	14	theme	vegetables	1647:1656	arg1	wrapping					1623:1630	wrapping	1623:1630	wrapping	1623:1630	Based on the improved tensile properties and photo resistivity, the cellulose/SCBP composite films with alkali treated SCBP may be considered for packaging and wrapping of flowers and vegetables.
28774586	10	14	theme	vegetables	1647:1656	arg1	packaging					1609:1617	packaging	1609:1617	packaging	1609:1617	Based on the improved tensile properties and photo resistivity, the cellulose/SCBP composite films with alkali treated SCBP may be considered for packaging and wrapping of flowers and vegetables.
28774586	3	15	theme	composite	706:714	arg1	films					716:720	the composite films	702:720	the composite films	702:720	Cellulose matrix was dissolved in the relatively safer aqueous solution mixture (8% LiOH and 15% Urea) precooled to -12.5°C. To the cellulose solution (SCBP) was added in 5-25wt% and the composite films were prepared by regeneration method using ethyl alcohol as a coagulant.
28774586	1	16	from	filler	355:360	arg1	biocomposites					365:377	biocomposites	365:377	biocomposites	365:377	As the annual production of the solid waste generable in the form of spent coffee bean powder (SCBP) is over 6 million tons, its utilization in the generation of green energy, waste water treatment and as a filler in biocomposites is desirable.
28774586	10	17	theme	flowers	1635:1641	arg1	wrapping					1623:1630	wrapping	1623:1630	wrapping	1623:1630	Based on the improved tensile properties and photo resistivity, the cellulose/SCBP composite films with alkali treated SCBP may be considered for packaging and wrapping of flowers and vegetables.
28774586	10	17	theme	flowers	1635:1641	arg1	packaging					1609:1617	packaging	1609:1617	packaging	1609:1617	Based on the improved tensile properties and photo resistivity, the cellulose/SCBP composite films with alkali treated SCBP may be considered for packaging and wrapping of flowers and vegetables.
28774586	2	18	theme	article	415:421	arg1	objective					397:405	The objective	393:405	The objective of this article	393:421	The objective of this article is to analyze the possibilities to valorize coffee bean powder as a filler in cellulose matrix.
28774586	0	19	theme	bean	70:73	arg1	powder					75:80	spent coffee bean powder	57:80	spent coffee bean powder	57:80	Utilization of chemically treated municipal solid waste (spent coffee bean powder) as reinforcement in cellulose matrix for packaging applications.
28774586	0	19	theme	bean	70:73	arg1	waste					50:54	municipal solid waste	34:54	chemically treated municipal solid waste (spent coffee bean powder)	15:81	Utilization of chemically treated municipal solid waste (spent coffee bean powder) as reinforcement in cellulose matrix for packaging applications.
28774586	0	20	from	Utilization	0:10	arg1	matrix					113:118	cellulose matrix	103:118	cellulose matrix for packaging applications	103:145	Utilization of chemically treated municipal solid waste (spent coffee bean powder) as reinforcement in cellulose matrix for packaging applications.
28774586	1	21	from	treatment	336:344	arg1	biocomposites					365:377	biocomposites	365:377	biocomposites	365:377	As the annual production of the solid waste generable in the form of spent coffee bean powder (SCBP) is over 6 million tons, its utilization in the generation of green energy, waste water treatment and as a filler in biocomposites is desirable.
28774586	5	22	theme	5	826:826	arg1	%					827:827	%	827:827	%	827:827	5% NaOH and the composite films were also prepared using alkali treated SCBP as a filler.
28774586	8	23	theme	tensile	1175:1181	arg1	strength					1183:1190	The maximum tensile strength	1163:1190	The maximum tensile strength of the composite films with alkali treated SCBP	1163:1238	The maximum tensile strength of the composite films with alkali treated SCBP varied between (106-149MPa) and increased with SCBP content when compared to the composites with untreated SCBP.
28774586	8	24	with	composites	1321:1330	arg1	SCBP					1347:1350	untreated SCBP	1337:1350	untreated SCBP	1337:1350	The maximum tensile strength of the composite films with alkali treated SCBP varied between (106-149MPa) and increased with SCBP content when compared to the composites with untreated SCBP.
28774586	7	25	theme	treated	1023:1029	arg1	SCBP					1031:1034	alkali treated SCBP	1016:1034	alkali treated SCBP	1016:1034	The cellulose/SCBP films without and with alkali treated SCBP were characterized by FTIR, XRD, optical and polarized optical microscopy, thermogravimetric analysis (TGA) and tensile tests.
28774586	1	26	theme	green	310:314	arg1	energy					316:321	green energy	310:321	green energy	310:321	As the annual production of the solid waste generable in the form of spent coffee bean powder (SCBP) is over 6 million tons, its utilization in the generation of green energy, waste water treatment and as a filler in biocomposites is desirable.
28774586	3	27	theme	ethyl	765:769	arg1	alcohol					771:777	ethyl alcohol	765:777	ethyl alcohol	765:777	Cellulose matrix was dissolved in the relatively safer aqueous solution mixture (8% LiOH and 15% Urea) precooled to -12.5°C. To the cellulose solution (SCBP) was added in 5-25wt% and the composite films were prepared by regeneration method using ethyl alcohol as a coagulant.
28774586	3	27	theme	ethyl	765:769	arg1	coagulant					784:792	a coagulant	782:792	a coagulant	782:792	Cellulose matrix was dissolved in the relatively safer aqueous solution mixture (8% LiOH and 15% Urea) precooled to -12.5°C. To the cellulose solution (SCBP) was added in 5-25wt% and the composite films were prepared by regeneration method using ethyl alcohol as a coagulant.
28774586	0	28	theme	coffee	63:68	arg1	powder					75:80	spent coffee bean powder	57:80	spent coffee bean powder	57:80	Utilization of chemically treated municipal solid waste (spent coffee bean powder) as reinforcement in cellulose matrix for packaging applications.
28774586	0	28	theme	coffee	63:68	arg1	waste					50:54	municipal solid waste	34:54	chemically treated municipal solid waste (spent coffee bean powder)	15:81	Utilization of chemically treated municipal solid waste (spent coffee bean powder) as reinforcement in cellulose matrix for packaging applications.
28774586	1	29	from	energy	316:321	arg1	biocomposites					365:377	biocomposites	365:377	biocomposites	365:377	As the annual production of the solid waste generable in the form of spent coffee bean powder (SCBP) is over 6 million tons, its utilization in the generation of green energy, waste water treatment and as a filler in biocomposites is desirable.
28774586	9	30	theme	composite	1382:1390	arg1	stability					1365:1373	The thermal stability	1353:1373	The thermal stability of the composite	1353:1390	The thermal stability of the composite was higher at elevated temperatures when alkali treated SCBP was used.
28774586	9	30	theme	composite	1382:1390	arg1	higher					1396:1401	higher	1396:1401	higher	1396:1401	The thermal stability of the composite was higher at elevated temperatures when alkali treated SCBP was used.
28774586	8	31	theme	composite	1199:1207	arg1	films					1209:1213	the composite films	1195:1213	the composite films with alkali treated SCBP	1195:1238	The maximum tensile strength of the composite films with alkali treated SCBP varied between (106-149MPa) and increased with SCBP content when compared to the composites with untreated SCBP.
28774586	3	32	theme	aqueous	574:580	arg1	solution					582:589	the relatively safer aqueous solution	553:589	the relatively safer aqueous solution	553:589	Cellulose matrix was dissolved in the relatively safer aqueous solution mixture (8% LiOH and 15% Urea) precooled to -12.5°C. To the cellulose solution (SCBP) was added in 5-25wt% and the composite films were prepared by regeneration method using ethyl alcohol as a coagulant.
28774586	10	33	theme	improved	1476:1483	arg1	properties					1493:1502	the improved tensile properties	1472:1502	the improved tensile properties	1472:1502	Based on the improved tensile properties and photo resistivity, the cellulose/SCBP composite films with alkali treated SCBP may be considered for packaging and wrapping of flowers and vegetables.
28774586	7	34	theme	cellulose/SCBP	978:991	arg1	films					993:997	The cellulose/SCBP films	974:997	The cellulose/SCBP films without and with alkali treated SCBP	974:1034	The cellulose/SCBP films without and with alkali treated SCBP were characterized by FTIR, XRD, optical and polarized optical microscopy, thermogravimetric analysis (TGA) and tensile tests.
28774586	0	35	theme	municipal	34:42	arg1	powder					75:80	spent coffee bean powder	57:80	spent coffee bean powder	57:80	Utilization of chemically treated municipal solid waste (spent coffee bean powder) as reinforcement in cellulose matrix for packaging applications.
28774586	0	35	theme	municipal	34:42	arg1	waste					50:54	municipal solid waste	34:54	chemically treated municipal solid waste (spent coffee bean powder)	15:81	Utilization of chemically treated municipal solid waste (spent coffee bean powder) as reinforcement in cellulose matrix for packaging applications.
28774586	9	36	theme	elevated	1406:1413	arg1	temperatures					1415:1426	elevated temperatures	1406:1426	elevated temperatures	1406:1426	The thermal stability of the composite was higher at elevated temperatures when alkali treated SCBP was used.
28774586	0	37	theme	spent	57:61	arg1	powder					75:80	spent coffee bean powder	57:80	spent coffee bean powder	57:80	Utilization of chemically treated municipal solid waste (spent coffee bean powder) as reinforcement in cellulose matrix for packaging applications.
28774586	0	37	theme	spent	57:61	arg1	waste					50:54	municipal solid waste	34:54	chemically treated municipal solid waste (spent coffee bean powder)	15:81	Utilization of chemically treated municipal solid waste (spent coffee bean powder) as reinforcement in cellulose matrix for packaging applications.
28774586	8	38	theme	treated	1227:1233	arg1	SCBP					1235:1238	alkali treated SCBP	1220:1238	alkali treated SCBP	1220:1238	The maximum tensile strength of the composite films with alkali treated SCBP varied between (106-149MPa) and increased with SCBP content when compared to the composites with untreated SCBP.
28774586	1	39	from	utilization	277:287	arg1	generation					296:305	the generation	292:305	the generation of green energy, waste water treatment and as a filler in biocomposites	292:377	As the annual production of the solid waste generable in the form of spent coffee bean powder (SCBP) is over 6 million tons, its utilization in the generation of green energy, waste water treatment and as a filler in biocomposites is desirable.
28774586	3	40	theme	8	600:600	arg1	%					601:601	%	601:601	%	601:601	Cellulose matrix was dissolved in the relatively safer aqueous solution mixture (8% LiOH and 15% Urea) precooled to -12.5°C. To the cellulose solution (SCBP) was added in 5-25wt% and the composite films were prepared by regeneration method using ethyl alcohol as a coagulant.
28774586	10	41	theme	photo	1508:1512	arg1	resistivity					1514:1524	photo resistivity	1508:1524	photo resistivity	1508:1524	Based on the improved tensile properties and photo resistivity, the cellulose/SCBP composite films with alkali treated SCBP may be considered for packaging and wrapping of flowers and vegetables.
28774586	10	42	with	films	1556:1560	arg1	SCBP					1582:1585	alkali treated SCBP	1567:1585	alkali treated SCBP	1567:1585	Based on the improved tensile properties and photo resistivity, the cellulose/SCBP composite films with alkali treated SCBP may be considered for packaging and wrapping of flowers and vegetables.
28774586	9	43	theme	treated	1440:1446	arg1	SCBP					1448:1451	alkali treated SCBP	1433:1451	alkali treated SCBP	1433:1451	The thermal stability of the composite was higher at elevated temperatures when alkali treated SCBP was used.
28774586	7	44	theme	optical	1091:1097	arg1	microscopy					1099:1108	FTIR, XRD, optical and polarized optical microscopy	1058:1108	FTIR, XRD, optical and polarized optical microscopy	1058:1108	The cellulose/SCBP films without and with alkali treated SCBP were characterized by FTIR, XRD, optical and polarized optical microscopy, thermogravimetric analysis (TGA) and tensile tests.
28774586	6	45	theme	composites	929:938	arg1	films					920:924	The films	916:924	The films of composites	916:938	The films of composites were uniform with brown in color.
28774586	3	46	theme	15	612:613	arg1	%					614:614	%	614:614	%	614:614	Cellulose matrix was dissolved in the relatively safer aqueous solution mixture (8% LiOH and 15% Urea) precooled to -12.5°C. To the cellulose solution (SCBP) was added in 5-25wt% and the composite films were prepared by regeneration method using ethyl alcohol as a coagulant.
28774586	10	47	theme	cellulose/SCBP	1531:1544	arg1	films					1556:1560	the cellulose/SCBP composite films	1527:1560	the cellulose/SCBP composite films with alkali treated SCBP	1527:1585	Based on the improved tensile properties and photo resistivity, the cellulose/SCBP composite films with alkali treated SCBP may be considered for packaging and wrapping of flowers and vegetables.
28774586	1	48	theme	coffee	223:228	arg1	SCBP					243:246	SCBP	243:246	SCBP	243:246	As the annual production of the solid waste generable in the form of spent coffee bean powder (SCBP) is over 6 million tons, its utilization in the generation of green energy, waste water treatment and as a filler in biocomposites is desirable.
28774586	1	48	theme	coffee	223:228	arg1	powder					235:240	spent coffee bean powder	217:240	spent coffee bean powder (SCBP)	217:247	As the annual production of the solid waste generable in the form of spent coffee bean powder (SCBP) is over 6 million tons, its utilization in the generation of green energy, waste water treatment and as a filler in biocomposites is desirable.
28774586	1	49	from	biocomposites	365:377	arg1	generation					296:305	the generation	292:305	the generation of green energy, waste water treatment and as a filler in biocomposites	292:377	As the annual production of the solid waste generable in the form of spent coffee bean powder (SCBP) is over 6 million tons, its utilization in the generation of green energy, waste water treatment and as a filler in biocomposites is desirable.
28774586	1	50	theme	powder	235:240	arg1	form					209:212	the form	205:212	the form of spent coffee bean powder (SCBP)	205:247	As the annual production of the solid waste generable in the form of spent coffee bean powder (SCBP) is over 6 million tons, its utilization in the generation of green energy, waste water treatment and as a filler in biocomposites is desirable.
28774586	2	51	theme	cellulose	501:509	arg1	matrix					511:516	cellulose matrix	501:516	cellulose matrix	501:516	The objective of this article is to analyze the possibilities to valorize coffee bean powder as a filler in cellulose matrix.
28774586	2	52	from	filler	491:496	arg1	matrix					511:516	cellulose matrix	501:516	cellulose matrix	501:516	The objective of this article is to analyze the possibilities to valorize coffee bean powder as a filler in cellulose matrix.
28774586	3	53	theme	precooled	622:630	arg1	mixture					591:597	mixture	591:597	mixture (8% LiOH and 15% Urea) precooled to -12.5°C. To the cellulose solution (SCBP)	591:675	Cellulose matrix was dissolved in the relatively safer aqueous solution mixture (8% LiOH and 15% Urea) precooled to -12.5°C. To the cellulose solution (SCBP) was added in 5-25wt% and the composite films were prepared by regeneration method using ethyl alcohol as a coagulant.
28774586	10	54	theme	alkali	1567:1572	arg1	SCBP					1582:1585	alkali treated SCBP	1567:1585	alkali treated SCBP	1567:1585	Based on the improved tensile properties and photo resistivity, the cellulose/SCBP composite films with alkali treated SCBP may be considered for packaging and wrapping of flowers and vegetables.
28774586	1	55	theme	annual	155:160	arg1	production					162:171	the annual production	151:171	the annual production of the solid waste generable in the form of spent coffee bean powder (SCBP)	151:247	As the annual production of the solid waste generable in the form of spent coffee bean powder (SCBP) is over 6 million tons, its utilization in the generation of green energy, waste water treatment and as a filler in biocomposites is desirable.
28774586	0	56	theme	waste	50:54	arg1	Utilization					0:10	Utilization	0:10	Utilization of chemically treated municipal solid waste (spent coffee bean powder) as reinforcement in cellulose matrix for packaging applications.	0:146	Utilization of chemically treated municipal solid waste (spent coffee bean powder) as reinforcement in cellulose matrix for packaging applications.
28774586	1	57	theme	solid	180:184	arg1	generable					192:200	the solid waste generable	176:200	the solid waste generable	176:200	As the annual production of the solid waste generable in the form of spent coffee bean powder (SCBP) is over 6 million tons, its utilization in the generation of green energy, waste water treatment and as a filler in biocomposites is desirable.
28774586	2	58	theme	bean	474:477	arg1	powder					479:484	coffee bean powder	467:484	coffee bean powder	467:484	The objective of this article is to analyze the possibilities to valorize coffee bean powder as a filler in cellulose matrix.
28774586	2	58	theme	bean	474:477	arg1	filler					491:496	a filler	489:496	a filler in cellulose matrix	489:516	The objective of this article is to analyze the possibilities to valorize coffee bean powder as a filler in cellulose matrix.
28774586	8	59	with	films	1209:1213	arg1	SCBP					1235:1238	alkali treated SCBP	1220:1238	alkali treated SCBP	1220:1238	The maximum tensile strength of the composite films with alkali treated SCBP varied between (106-149MPa) and increased with SCBP content when compared to the composites with untreated SCBP.
28774586	0	60	theme	packaging	124:132	arg1	applications					134:145	packaging applications	124:145	packaging applications	124:145	Utilization of chemically treated municipal solid waste (spent coffee bean powder) as reinforcement in cellulose matrix for packaging applications.
28774586	1	61	theme	waste	324:328	arg1	treatment					336:344	waste water treatment	324:344	waste water treatment	324:344	As the annual production of the solid waste generable in the form of spent coffee bean powder (SCBP) is over 6 million tons, its utilization in the generation of green energy, waste water treatment and as a filler in biocomposites is desirable.
28774586	1	62	theme	generable	192:200	arg1	production					162:171	the annual production	151:171	the annual production of the solid waste generable in the form of spent coffee bean powder (SCBP)	151:247	As the annual production of the solid waste generable in the form of spent coffee bean powder (SCBP) is over 6 million tons, its utilization in the generation of green energy, waste water treatment and as a filler in biocomposites is desirable.
28774586	1	63	theme	treatment	336:344	arg1	generation					296:305	the generation	292:305	the generation of green energy, waste water treatment and as a filler in biocomposites	292:377	As the annual production of the solid waste generable in the form of spent coffee bean powder (SCBP) is over 6 million tons, its utilization in the generation of green energy, waste water treatment and as a filler in biocomposites is desirable.
28774586	8	64	theme	SCBP	1287:1290	arg1	content					1292:1298	SCBP content	1287:1298	SCBP content	1287:1298	The maximum tensile strength of the composite films with alkali treated SCBP varied between (106-149MPa) and increased with SCBP content when compared to the composites with untreated SCBP.
28774586	5	65	theme	treated	890:896	arg1	SCBP					898:901	alkali treated SCBP	883:901	alkali treated SCBP	883:901	5% NaOH and the composite films were also prepared using alkali treated SCBP as a filler.
28774586	5	65	theme	treated	890:896	arg1	filler					908:913	a filler	906:913	a filler	906:913	5% NaOH and the composite films were also prepared using alkali treated SCBP as a filler.
28774586	7	66	theme	FTIR	1058:1061	arg1	microscopy					1099:1108	FTIR, XRD, optical and polarized optical microscopy	1058:1108	FTIR, XRD, optical and polarized optical microscopy	1058:1108	The cellulose/SCBP films without and with alkali treated SCBP were characterized by FTIR, XRD, optical and polarized optical microscopy, thermogravimetric analysis (TGA) and tensile tests.
28774586	1	67	theme	filler	355:360	arg1	generation					296:305	the generation	292:305	the generation of green energy, waste water treatment and as a filler in biocomposites	292:377	As the annual production of the solid waste generable in the form of spent coffee bean powder (SCBP) is over 6 million tons, its utilization in the generation of green energy, waste water treatment and as a filler in biocomposites is desirable.
28774586	1	68	from	production	162:171	arg1	form					209:212	the form	205:212	the form of spent coffee bean powder (SCBP)	205:247	As the annual production of the solid waste generable in the form of spent coffee bean powder (SCBP) is over 6 million tons, its utilization in the generation of green energy, waste water treatment and as a filler in biocomposites is desirable.
28774586	3	69	theme	regeneration	739:750	arg1	method					752:757	regeneration method	739:757	regeneration method using ethyl alcohol as a coagulant	739:792	Cellulose matrix was dissolved in the relatively safer aqueous solution mixture (8% LiOH and 15% Urea) precooled to -12.5°C. To the cellulose solution (SCBP) was added in 5-25wt% and the composite films were prepared by regeneration method using ethyl alcohol as a coagulant.
28774586	8	70	theme	untreated	1337:1345	arg1	SCBP					1347:1350	untreated SCBP	1337:1350	untreated SCBP	1337:1350	The maximum tensile strength of the composite films with alkali treated SCBP varied between (106-149MPa) and increased with SCBP content when compared to the composites with untreated SCBP.
28774586	9	71	theme	thermal	1357:1363	arg1	stability					1365:1373	The thermal stability	1353:1373	The thermal stability of the composite	1353:1390	The thermal stability of the composite was higher at elevated temperatures when alkali treated SCBP was used.
28774586	9	71	theme	thermal	1357:1363	arg1	higher					1396:1401	higher	1396:1401	higher	1396:1401	The thermal stability of the composite was higher at elevated temperatures when alkali treated SCBP was used.
28774586	7	72	theme	alkali	1016:1021	arg1	SCBP					1031:1034	alkali treated SCBP	1016:1034	alkali treated SCBP	1016:1034	The cellulose/SCBP films without and with alkali treated SCBP were characterized by FTIR, XRD, optical and polarized optical microscopy, thermogravimetric analysis (TGA) and tensile tests.
28774586	5	73	theme	%	827:827	arg1	NaOH					829:832	5% NaOH	826:832	5% NaOH	826:832	5% NaOH and the composite films were also prepared using alkali treated SCBP as a filler.
28774586	7	74	theme	tensile	1148:1154	arg1	tests					1156:1160	tensile tests	1148:1160	tensile tests	1148:1160	The cellulose/SCBP films without and with alkali treated SCBP were characterized by FTIR, XRD, optical and polarized optical microscopy, thermogravimetric analysis (TGA) and tensile tests.
28774586	8	75	theme	maximum	1167:1173	arg1	strength					1183:1190	The maximum tensile strength	1163:1190	The maximum tensile strength of the composite films with alkali treated SCBP	1163:1238	The maximum tensile strength of the composite films with alkali treated SCBP varied between (106-149MPa) and increased with SCBP content when compared to the composites with untreated SCBP.
28774586	1	76	theme	energy	316:321	arg1	generation					296:305	the generation	292:305	the generation of green energy, waste water treatment and as a filler in biocomposites	292:377	As the annual production of the solid waste generable in the form of spent coffee bean powder (SCBP) is over 6 million tons, its utilization in the generation of green energy, waste water treatment and as a filler in biocomposites is desirable.
28774586	0	77	theme	treated	26:32	arg1	powder					75:80	spent coffee bean powder	57:80	spent coffee bean powder	57:80	Utilization of chemically treated municipal solid waste (spent coffee bean powder) as reinforcement in cellulose matrix for packaging applications.
28774586	0	77	theme	treated	26:32	arg1	waste					50:54	municipal solid waste	34:54	chemically treated municipal solid waste (spent coffee bean powder)	15:81	Utilization of chemically treated municipal solid waste (spent coffee bean powder) as reinforcement in cellulose matrix for packaging applications.
28774586	3	78	theme	safer	568:572	arg1	solution					582:589	the relatively safer aqueous solution	553:589	the relatively safer aqueous solution	553:589	Cellulose matrix was dissolved in the relatively safer aqueous solution mixture (8% LiOH and 15% Urea) precooled to -12.5°C. To the cellulose solution (SCBP) was added in 5-25wt% and the composite films were prepared by regeneration method using ethyl alcohol as a coagulant.
28774586	10	79	theme	tensile	1485:1491	arg1	properties					1493:1502	the improved tensile properties	1472:1502	the improved tensile properties	1472:1502	Based on the improved tensile properties and photo resistivity, the cellulose/SCBP composite films with alkali treated SCBP may be considered for packaging and wrapping of flowers and vegetables.
28774586	0	80	theme	solid	44:48	arg1	powder					75:80	spent coffee bean powder	57:80	spent coffee bean powder	57:80	Utilization of chemically treated municipal solid waste (spent coffee bean powder) as reinforcement in cellulose matrix for packaging applications.
28774586	0	80	theme	solid	44:48	arg1	waste					50:54	municipal solid waste	34:54	chemically treated municipal solid waste (spent coffee bean powder)	15:81	Utilization of chemically treated municipal solid waste (spent coffee bean powder) as reinforcement in cellulose matrix for packaging applications.
28774586	7	81	theme	thermogravimetric	1111:1127	arg1	TGA					1139:1141	TGA	1139:1141	TGA	1139:1141	The cellulose/SCBP films without and with alkali treated SCBP were characterized by FTIR, XRD, optical and polarized optical microscopy, thermogravimetric analysis (TGA) and tensile tests.
28774586	7	81	theme	thermogravimetric	1111:1127	arg1	analysis					1129:1136	thermogravimetric analysis	1111:1136	thermogravimetric analysis (TGA)	1111:1142	The cellulose/SCBP films without and with alkali treated SCBP were characterized by FTIR, XRD, optical and polarized optical microscopy, thermogravimetric analysis (TGA) and tensile tests.
28774586	8	82	theme	films	1209:1213	arg1	strength					1183:1190	The maximum tensile strength	1163:1190	The maximum tensile strength of the composite films with alkali treated SCBP	1163:1238	The maximum tensile strength of the composite films with alkali treated SCBP varied between (106-149MPa) and increased with SCBP content when compared to the composites with untreated SCBP.
28774586	1	83	theme	million	259:265	arg1	tons					267:270	6 million tons	257:270	6 million tons	257:270	As the annual production of the solid waste generable in the form of spent coffee bean powder (SCBP) is over 6 million tons, its utilization in the generation of green energy, waste water treatment and as a filler in biocomposites is desirable.
28774586	8	84	theme	alkali	1220:1225	arg1	SCBP					1235:1238	alkali treated SCBP	1220:1238	alkali treated SCBP	1220:1238	The maximum tensile strength of the composite films with alkali treated SCBP varied between (106-149MPa) and increased with SCBP content when compared to the composites with untreated SCBP.
28774586	9	85	theme	alkali	1433:1438	arg1	SCBP					1448:1451	alkali treated SCBP	1433:1451	alkali treated SCBP	1433:1451	The thermal stability of the composite was higher at elevated temperatures when alkali treated SCBP was used.
28774586	3	86	theme	Cellulose	519:527	arg1	matrix					529:534	Cellulose matrix	519:534	Cellulose matrix	519:534	Cellulose matrix was dissolved in the relatively safer aqueous solution mixture (8% LiOH and 15% Urea) precooled to -12.5°C. To the cellulose solution (SCBP) was added in 5-25wt% and the composite films were prepared by regeneration method using ethyl alcohol as a coagulant.
28774586	7	87	theme	polarized	1081:1089	arg1	microscopy					1099:1108	FTIR, XRD, optical and polarized optical microscopy	1058:1108	FTIR, XRD, optical and polarized optical microscopy	1058:1108	The cellulose/SCBP films without and with alkali treated SCBP were characterized by FTIR, XRD, optical and polarized optical microscopy, thermogravimetric analysis (TGA) and tensile tests.
28774586	5	88	theme	composite	842:850	arg1	films					852:856	the composite films	838:856	the composite films	838:856	5% NaOH and the composite films were also prepared using alkali treated SCBP as a filler.
28774586	7	89	theme	optical	1069:1075	arg1	microscopy					1099:1108	FTIR, XRD, optical and polarized optical microscopy	1058:1108	FTIR, XRD, optical and polarized optical microscopy	1058:1108	The cellulose/SCBP films without and with alkali treated SCBP were characterized by FTIR, XRD, optical and polarized optical microscopy, thermogravimetric analysis (TGA) and tensile tests.
28774586	10	90	theme	composite	1546:1554	arg1	films					1556:1560	the cellulose/SCBP composite films	1527:1560	the cellulose/SCBP composite films with alkali treated SCBP	1527:1585	Based on the improved tensile properties and photo resistivity, the cellulose/SCBP composite films with alkali treated SCBP may be considered for packaging and wrapping of flowers and vegetables.
28774586	1	91	theme	spent	217:221	arg1	SCBP					243:246	SCBP	243:246	SCBP	243:246	As the annual production of the solid waste generable in the form of spent coffee bean powder (SCBP) is over 6 million tons, its utilization in the generation of green energy, waste water treatment and as a filler in biocomposites is desirable.
28774586	1	91	theme	spent	217:221	arg1	powder					235:240	spent coffee bean powder	217:240	spent coffee bean powder (SCBP)	217:247	As the annual production of the solid waste generable in the form of spent coffee bean powder (SCBP) is over 6 million tons, its utilization in the generation of green energy, waste water treatment and as a filler in biocomposites is desirable.
28433181	4	0	theme	SPS2p	1051:1055	arg1	inhibition					1037:1046	the inhibition	1033:1046	the inhibition of SPS2p on the PI3K/AKT pathway	1033:1079	These results indicate that SPS2p can inhibit the proliferation and EMT, and promote the apoptosis in HT29 cells, which may be related to the inhibition of SPS2p on the PI3K/AKT pathway.
28433181	2	1	theme	arabinose	447:455	arg1	presence					435:442	the presence	431:442	the presence of arabinose, mannose, glucose and galactose	431:487	The molecular weight of SPS2p showed only one molecular weight distribution (2.6×104Da) and the monosaccharide composition of SPS2p showed the presence of arabinose, mannose, glucose and galactose at the ratio of 1.31:1.00:3.59:1.59.
28433181	1	2	dep	%	259:259	arg1	acid					268:271	uronic acid	261:271	38.5% uronic acid	255:271	A water-soluble polysaccharide SPS2p was isolated from the whole grass of Scutellaria barbata and SPS2p contained 53.6% carbohydrates, 38.5% uronic acid and 8.2% proteins.
28433181	2	3	theme	SPS2p	316:320	arg1	weight					306:311	The molecular weight	292:311	The molecular weight of SPS2p	292:320	The molecular weight of SPS2p showed only one molecular weight distribution (2.6×104Da) and the monosaccharide composition of SPS2p showed the presence of arabinose, mannose, glucose and galactose at the ratio of 1.31:1.00:3.59:1.59.
28433181	0	4	theme	human	89:93	arg1	Cells					113:117	human colon cancer HT29 Cells	89:117	human colon cancer HT29 Cells	89:117	Effects of Scutellaria barbata polysaccharide on the proliferation, apoptosis and EMT of human colon cancer HT29 Cells.
28433181	0	5	from	Effects	0:6	arg1	EMT					82:84	EMT	82:84	EMT	82:84	Effects of Scutellaria barbata polysaccharide on the proliferation, apoptosis and EMT of human colon cancer HT29 Cells.
28433181	0	5	from	Effects	0:6	arg1	apoptosis					68:76	apoptosis	68:76	apoptosis	68:76	Effects of Scutellaria barbata polysaccharide on the proliferation, apoptosis and EMT of human colon cancer HT29 Cells.
28433181	0	5	from	Effects	0:6	arg1	proliferation					53:65	proliferation	53:65	proliferation	53:65	Effects of Scutellaria barbata polysaccharide on the proliferation, apoptosis and EMT of human colon cancer HT29 Cells.
28433181	2	6	dep	showed	322:327	arg1	showed					424:429	showed	424:429	showed only one molecular weight distribution (2.6×104Da) and the monosaccharide composition of SPS2p showed the presence of arabinose, mannose, glucose and galactose at the ratio of 1.31:1.00:3.59:1.59	322:523	The molecular weight of SPS2p showed only one molecular weight distribution (2.6×104Da) and the monosaccharide composition of SPS2p showed the presence of arabinose, mannose, glucose and galactose at the ratio of 1.31:1.00:3.59:1.59.
28433181	3	7	theme	Bax	680:682	arg1	rate					640:643	apoptosis rate	630:643	apoptosis rate	630:643	The results showed that SPS2p could improve the proliferation inhibition rate; SPS2p could also elevate apoptosis rate, apoptosis index and the levels of Bax and Bak, but lower levels of Bcl-2 and FN; SPS2p could up-regulate the expression of E-cadherin mRNA, and down-regulate the expressions of N-cadherin and vimentin mRNA, and the ratio of p-AKT/AKT in HT29 cells.
28433181	3	7	theme	Bax	680:682	arg1	index					656:660	apoptosis index	646:660	apoptosis index	646:660	The results showed that SPS2p could improve the proliferation inhibition rate; SPS2p could also elevate apoptosis rate, apoptosis index and the levels of Bax and Bak, but lower levels of Bcl-2 and FN; SPS2p could up-regulate the expression of E-cadherin mRNA, and down-regulate the expressions of N-cadherin and vimentin mRNA, and the ratio of p-AKT/AKT in HT29 cells.
28433181	3	7	theme	Bax	680:682	arg1	levels					670:675	the levels	666:675	the levels of Bax and Bak	666:690	The results showed that SPS2p could improve the proliferation inhibition rate; SPS2p could also elevate apoptosis rate, apoptosis index and the levels of Bax and Bak, but lower levels of Bcl-2 and FN; SPS2p could up-regulate the expression of E-cadherin mRNA, and down-regulate the expressions of N-cadherin and vimentin mRNA, and the ratio of p-AKT/AKT in HT29 cells.
28433181	3	8	theme	vimentin	838:845	arg1	expressions					808:818	the expressions	804:818	the expressions of N-cadherin and vimentin mRNA	804:850	The results showed that SPS2p could improve the proliferation inhibition rate; SPS2p could also elevate apoptosis rate, apoptosis index and the levels of Bax and Bak, but lower levels of Bcl-2 and FN; SPS2p could up-regulate the expression of E-cadherin mRNA, and down-regulate the expressions of N-cadherin and vimentin mRNA, and the ratio of p-AKT/AKT in HT29 cells.
28433181	3	8	theme	vimentin	838:845	arg1	ratio					861:865	the ratio	857:865	the ratio of p-AKT/AKT in HT29 cells	857:892	The results showed that SPS2p could improve the proliferation inhibition rate; SPS2p could also elevate apoptosis rate, apoptosis index and the levels of Bax and Bak, but lower levels of Bcl-2 and FN; SPS2p could up-regulate the expression of E-cadherin mRNA, and down-regulate the expressions of N-cadherin and vimentin mRNA, and the ratio of p-AKT/AKT in HT29 cells.
28433181	0	9	theme	cancer	101:106	arg1	Cells					113:117	human colon cancer HT29 Cells	89:117	human colon cancer HT29 Cells	89:117	Effects of Scutellaria barbata polysaccharide on the proliferation, apoptosis and EMT of human colon cancer HT29 Cells.
28433181	3	10	from	expressions	808:818	arg1	cells					888:892	HT29 cells	883:892	HT29 cells	883:892	The results showed that SPS2p could improve the proliferation inhibition rate; SPS2p could also elevate apoptosis rate, apoptosis index and the levels of Bax and Bak, but lower levels of Bcl-2 and FN; SPS2p could up-regulate the expression of E-cadherin mRNA, and down-regulate the expressions of N-cadherin and vimentin mRNA, and the ratio of p-AKT/AKT in HT29 cells.
28433181	4	11	theme	HT29	997:1000	arg1	cells					1002:1006	HT29 cells	997:1006	HT29 cells	997:1006	These results indicate that SPS2p can inhibit the proliferation and EMT, and promote the apoptosis in HT29 cells, which may be related to the inhibition of SPS2p on the PI3K/AKT pathway.
28433181	3	12	from	cells	888:892	arg1	expressions					808:818	the expressions	804:818	the expressions of N-cadherin and vimentin mRNA	804:850	The results showed that SPS2p could improve the proliferation inhibition rate; SPS2p could also elevate apoptosis rate, apoptosis index and the levels of Bax and Bak, but lower levels of Bcl-2 and FN; SPS2p could up-regulate the expression of E-cadherin mRNA, and down-regulate the expressions of N-cadherin and vimentin mRNA, and the ratio of p-AKT/AKT in HT29 cells.
28433181	3	12	from	cells	888:892	arg1	ratio					861:865	the ratio	857:865	the ratio of p-AKT/AKT in HT29 cells	857:892	The results showed that SPS2p could improve the proliferation inhibition rate; SPS2p could also elevate apoptosis rate, apoptosis index and the levels of Bax and Bak, but lower levels of Bcl-2 and FN; SPS2p could up-regulate the expression of E-cadherin mRNA, and down-regulate the expressions of N-cadherin and vimentin mRNA, and the ratio of p-AKT/AKT in HT29 cells.
28433181	0	13	theme	colon	95:99	arg1	Cells					113:117	human colon cancer HT29 Cells	89:117	human colon cancer HT29 Cells	89:117	Effects of Scutellaria barbata polysaccharide on the proliferation, apoptosis and EMT of human colon cancer HT29 Cells.
28433181	3	14	theme	N-cadherin	823:832	arg1	expressions					808:818	the expressions	804:818	the expressions of N-cadherin and vimentin mRNA	804:850	The results showed that SPS2p could improve the proliferation inhibition rate; SPS2p could also elevate apoptosis rate, apoptosis index and the levels of Bax and Bak, but lower levels of Bcl-2 and FN; SPS2p could up-regulate the expression of E-cadherin mRNA, and down-regulate the expressions of N-cadherin and vimentin mRNA, and the ratio of p-AKT/AKT in HT29 cells.
28433181	3	14	theme	N-cadherin	823:832	arg1	ratio					861:865	the ratio	857:865	the ratio of p-AKT/AKT in HT29 cells	857:892	The results showed that SPS2p could improve the proliferation inhibition rate; SPS2p could also elevate apoptosis rate, apoptosis index and the levels of Bax and Bak, but lower levels of Bcl-2 and FN; SPS2p could up-regulate the expression of E-cadherin mRNA, and down-regulate the expressions of N-cadherin and vimentin mRNA, and the ratio of p-AKT/AKT in HT29 cells.
28433181	2	15	theme	SPS2p	418:422	arg1	distribution					355:366	only one molecular weight distribution	329:366	only one molecular weight distribution (2.6×104Da)	329:378	The molecular weight of SPS2p showed only one molecular weight distribution (2.6×104Da) and the monosaccharide composition of SPS2p showed the presence of arabinose, mannose, glucose and galactose at the ratio of 1.31:1.00:3.59:1.59.
28433181	2	15	theme	SPS2p	418:422	arg1	composition					403:413	the monosaccharide composition	384:413	the monosaccharide composition of SPS2p	384:422	The molecular weight of SPS2p showed only one molecular weight distribution (2.6×104Da) and the monosaccharide composition of SPS2p showed the presence of arabinose, mannose, glucose and galactose at the ratio of 1.31:1.00:3.59:1.59.
28433181	2	15	theme	SPS2p	418:422	arg1	2.6×104Da					369:377	2.6×104Da	369:377	2.6×104Da	369:377	The molecular weight of SPS2p showed only one molecular weight distribution (2.6×104Da) and the monosaccharide composition of SPS2p showed the presence of arabinose, mannose, glucose and galactose at the ratio of 1.31:1.00:3.59:1.59.
28433181	1	16	dep	%	280:280	arg1	proteins					282:289	proteins	282:289	8.2% proteins	277:289	A water-soluble polysaccharide SPS2p was isolated from the whole grass of Scutellaria barbata and SPS2p contained 53.6% carbohydrates, 38.5% uronic acid and 8.2% proteins.
28433181	2	17	theme	weight	348:353	arg1	distribution					355:366	only one molecular weight distribution	329:366	only one molecular weight distribution (2.6×104Da)	329:378	The molecular weight of SPS2p showed only one molecular weight distribution (2.6×104Da) and the monosaccharide composition of SPS2p showed the presence of arabinose, mannose, glucose and galactose at the ratio of 1.31:1.00:3.59:1.59.
28433181	2	17	theme	weight	348:353	arg1	2.6×104Da					369:377	2.6×104Da	369:377	2.6×104Da	369:377	The molecular weight of SPS2p showed only one molecular weight distribution (2.6×104Da) and the monosaccharide composition of SPS2p showed the presence of arabinose, mannose, glucose and galactose at the ratio of 1.31:1.00:3.59:1.59.
28433181	3	18	theme	apoptosis	646:654	arg1	index					656:660	apoptosis index	646:660	apoptosis index	646:660	The results showed that SPS2p could improve the proliferation inhibition rate; SPS2p could also elevate apoptosis rate, apoptosis index and the levels of Bax and Bak, but lower levels of Bcl-2 and FN; SPS2p could up-regulate the expression of E-cadherin mRNA, and down-regulate the expressions of N-cadherin and vimentin mRNA, and the ratio of p-AKT/AKT in HT29 cells.
28433181	1	19	theme	whole	179:183	arg1	grass					185:189	the whole grass	175:189	the whole grass of Scutellaria barbata	175:212	A water-soluble polysaccharide SPS2p was isolated from the whole grass of Scutellaria barbata and SPS2p contained 53.6% carbohydrates, 38.5% uronic acid and 8.2% proteins.
28433181	0	20	theme	barbata	23:29	arg1	polysaccharide					31:44	Scutellaria barbata polysaccharide	11:44	Scutellaria barbata polysaccharide	11:44	Effects of Scutellaria barbata polysaccharide on the proliferation, apoptosis and EMT of human colon cancer HT29 Cells.
28433181	1	21	theme	uronic	261:266	arg1	acid					268:271	uronic acid	261:271	38.5% uronic acid	255:271	A water-soluble polysaccharide SPS2p was isolated from the whole grass of Scutellaria barbata and SPS2p contained 53.6% carbohydrates, 38.5% uronic acid and 8.2% proteins.
28433181	0	22	theme	Cells	113:117	arg1	EMT					82:84	EMT	82:84	EMT	82:84	Effects of Scutellaria barbata polysaccharide on the proliferation, apoptosis and EMT of human colon cancer HT29 Cells.
28433181	0	22	theme	Cells	113:117	arg1	apoptosis					68:76	apoptosis	68:76	apoptosis	68:76	Effects of Scutellaria barbata polysaccharide on the proliferation, apoptosis and EMT of human colon cancer HT29 Cells.
28433181	0	22	theme	Cells	113:117	arg1	proliferation					53:65	proliferation	53:65	proliferation	53:65	Effects of Scutellaria barbata polysaccharide on the proliferation, apoptosis and EMT of human colon cancer HT29 Cells.
28433181	2	23	theme	monosaccharide	388:401	arg1	composition					403:413	the monosaccharide composition	384:413	the monosaccharide composition of SPS2p	384:422	The molecular weight of SPS2p showed only one molecular weight distribution (2.6×104Da) and the monosaccharide composition of SPS2p showed the presence of arabinose, mannose, glucose and galactose at the ratio of 1.31:1.00:3.59:1.59.
28433181	3	24	theme	Bak	688:690	arg1	rate					640:643	apoptosis rate	630:643	apoptosis rate	630:643	The results showed that SPS2p could improve the proliferation inhibition rate; SPS2p could also elevate apoptosis rate, apoptosis index and the levels of Bax and Bak, but lower levels of Bcl-2 and FN; SPS2p could up-regulate the expression of E-cadherin mRNA, and down-regulate the expressions of N-cadherin and vimentin mRNA, and the ratio of p-AKT/AKT in HT29 cells.
28433181	3	24	theme	Bak	688:690	arg1	index					656:660	apoptosis index	646:660	apoptosis index	646:660	The results showed that SPS2p could improve the proliferation inhibition rate; SPS2p could also elevate apoptosis rate, apoptosis index and the levels of Bax and Bak, but lower levels of Bcl-2 and FN; SPS2p could up-regulate the expression of E-cadherin mRNA, and down-regulate the expressions of N-cadherin and vimentin mRNA, and the ratio of p-AKT/AKT in HT29 cells.
28433181	3	24	theme	Bak	688:690	arg1	levels					670:675	the levels	666:675	the levels of Bax and Bak	666:690	The results showed that SPS2p could improve the proliferation inhibition rate; SPS2p could also elevate apoptosis rate, apoptosis index and the levels of Bax and Bak, but lower levels of Bcl-2 and FN; SPS2p could up-regulate the expression of E-cadherin mRNA, and down-regulate the expressions of N-cadherin and vimentin mRNA, and the ratio of p-AKT/AKT in HT29 cells.
28433181	0	25	theme	HT29	108:111	arg1	Cells					113:117	human colon cancer HT29 Cells	89:117	human colon cancer HT29 Cells	89:117	Effects of Scutellaria barbata polysaccharide on the proliferation, apoptosis and EMT of human colon cancer HT29 Cells.
28433181	3	26	from	p-AKT/AKT	870:878	arg1	cells					888:892	HT29 cells	883:892	HT29 cells	883:892	The results showed that SPS2p could improve the proliferation inhibition rate; SPS2p could also elevate apoptosis rate, apoptosis index and the levels of Bax and Bak, but lower levels of Bcl-2 and FN; SPS2p could up-regulate the expression of E-cadherin mRNA, and down-regulate the expressions of N-cadherin and vimentin mRNA, and the ratio of p-AKT/AKT in HT29 cells.
28433181	4	27	from	inhibition	1037:1046	arg1	pathway					1073:1079	the PI3K/AKT pathway	1060:1079	the PI3K/AKT pathway	1060:1079	These results indicate that SPS2p can inhibit the proliferation and EMT, and promote the apoptosis in HT29 cells, which may be related to the inhibition of SPS2p on the PI3K/AKT pathway.
28433181	1	28	attach	isolated	161:168	arg2	SPS2p					151:155	A water-soluble polysaccharide SPS2p	120:155	A water-soluble polysaccharide SPS2p	120:155	A water-soluble polysaccharide SPS2p was isolated from the whole grass of Scutellaria barbata and SPS2p contained 53.6% carbohydrates, 38.5% uronic acid and 8.2% proteins.
28433181	1	28	attach	isolated	161:168	arg1	grass					185:189	the whole grass	175:189	the whole grass of Scutellaria barbata	175:212	A water-soluble polysaccharide SPS2p was isolated from the whole grass of Scutellaria barbata and SPS2p contained 53.6% carbohydrates, 38.5% uronic acid and 8.2% proteins.
28433181	2	29	theme	molecular	338:346	arg1	distribution					355:366	only one molecular weight distribution	329:366	only one molecular weight distribution (2.6×104Da)	329:378	The molecular weight of SPS2p showed only one molecular weight distribution (2.6×104Da) and the monosaccharide composition of SPS2p showed the presence of arabinose, mannose, glucose and galactose at the ratio of 1.31:1.00:3.59:1.59.
28433181	2	29	theme	molecular	338:346	arg1	2.6×104Da					369:377	2.6×104Da	369:377	2.6×104Da	369:377	The molecular weight of SPS2p showed only one molecular weight distribution (2.6×104Da) and the monosaccharide composition of SPS2p showed the presence of arabinose, mannose, glucose and galactose at the ratio of 1.31:1.00:3.59:1.59.
28433181	1	30	theme	Scutellaria	194:204	arg1	barbata					206:212	Scutellaria barbata	194:212	Scutellaria barbata	194:212	A water-soluble polysaccharide SPS2p was isolated from the whole grass of Scutellaria barbata and SPS2p contained 53.6% carbohydrates, 38.5% uronic acid and 8.2% proteins.
28433181	0	31	theme	polysaccharide	31:44	arg1	Effects					0:6	Effects	0:6	Effects of Scutellaria barbata polysaccharide on the proliferation, apoptosis and EMT of human colon cancer HT29 Cells	0:117	Effects of Scutellaria barbata polysaccharide on the proliferation, apoptosis and EMT of human colon cancer HT29 Cells.
28433181	3	32	theme	Bcl-2	713:717	arg1	levels					703:708	lower levels	697:708	lower levels of Bcl-2 and FN	697:724	The results showed that SPS2p could improve the proliferation inhibition rate; SPS2p could also elevate apoptosis rate, apoptosis index and the levels of Bax and Bak, but lower levels of Bcl-2 and FN; SPS2p could up-regulate the expression of E-cadherin mRNA, and down-regulate the expressions of N-cadherin and vimentin mRNA, and the ratio of p-AKT/AKT in HT29 cells.
28433181	1	33	contain	contained	224:232	arg2	%					259:259	38.5%	255:259	38.5% uronic acid	255:271	A water-soluble polysaccharide SPS2p was isolated from the whole grass of Scutellaria barbata and SPS2p contained 53.6% carbohydrates, 38.5% uronic acid and 8.2% proteins.
28433181	1	33	contain	contained	224:232	arg2	%					280:280	8.2%	277:280	8.2% proteins	277:289	A water-soluble polysaccharide SPS2p was isolated from the whole grass of Scutellaria barbata and SPS2p contained 53.6% carbohydrates, 38.5% uronic acid and 8.2% proteins.
28433181	1	33	contain	contained	224:232	arg1	SPS2p					218:222	SPS2p	218:222	SPS2p	218:222	A water-soluble polysaccharide SPS2p was isolated from the whole grass of Scutellaria barbata and SPS2p contained 53.6% carbohydrates, 38.5% uronic acid and 8.2% proteins.
28433181	1	33	contain	contained	224:232	arg2	%					238:238	53.6%	234:238	53.6% carbohydrates	234:252	A water-soluble polysaccharide SPS2p was isolated from the whole grass of Scutellaria barbata and SPS2p contained 53.6% carbohydrates, 38.5% uronic acid and 8.2% proteins.
28433181	3	34	dep	N-cadherin	823:832	arg1	mRNA					847:850	mRNA	847:850	mRNA	847:850	The results showed that SPS2p could improve the proliferation inhibition rate; SPS2p could also elevate apoptosis rate, apoptosis index and the levels of Bax and Bak, but lower levels of Bcl-2 and FN; SPS2p could up-regulate the expression of E-cadherin mRNA, and down-regulate the expressions of N-cadherin and vimentin mRNA, and the ratio of p-AKT/AKT in HT29 cells.
28433181	1	35	theme	barbata	206:212	arg1	grass					185:189	the whole grass	175:189	the whole grass of Scutellaria barbata	175:212	A water-soluble polysaccharide SPS2p was isolated from the whole grass of Scutellaria barbata and SPS2p contained 53.6% carbohydrates, 38.5% uronic acid and 8.2% proteins.
28433181	3	36	theme	lower	697:701	arg1	levels					703:708	lower levels	697:708	lower levels of Bcl-2 and FN	697:724	The results showed that SPS2p could improve the proliferation inhibition rate; SPS2p could also elevate apoptosis rate, apoptosis index and the levels of Bax and Bak, but lower levels of Bcl-2 and FN; SPS2p could up-regulate the expression of E-cadherin mRNA, and down-regulate the expressions of N-cadherin and vimentin mRNA, and the ratio of p-AKT/AKT in HT29 cells.
28433181	3	37	theme	HT29	883:886	arg1	cells					888:892	HT29 cells	883:892	HT29 cells	883:892	The results showed that SPS2p could improve the proliferation inhibition rate; SPS2p could also elevate apoptosis rate, apoptosis index and the levels of Bax and Bak, but lower levels of Bcl-2 and FN; SPS2p could up-regulate the expression of E-cadherin mRNA, and down-regulate the expressions of N-cadherin and vimentin mRNA, and the ratio of p-AKT/AKT in HT29 cells.
28433181	2	38	theme	1.31:1.00:3.59:1.59	505:523	arg1	ratio					496:500	the ratio	492:500	the ratio of 1.31:1.00:3.59:1.59	492:523	The molecular weight of SPS2p showed only one molecular weight distribution (2.6×104Da) and the monosaccharide composition of SPS2p showed the presence of arabinose, mannose, glucose and galactose at the ratio of 1.31:1.00:3.59:1.59.
28433181	3	39	from	ratio	861:865	arg1	cells					888:892	HT29 cells	883:892	HT29 cells	883:892	The results showed that SPS2p could improve the proliferation inhibition rate; SPS2p could also elevate apoptosis rate, apoptosis index and the levels of Bax and Bak, but lower levels of Bcl-2 and FN; SPS2p could up-regulate the expression of E-cadherin mRNA, and down-regulate the expressions of N-cadherin and vimentin mRNA, and the ratio of p-AKT/AKT in HT29 cells.
28433181	3	40	theme	proliferation	574:586	arg1	rate					599:602	the proliferation inhibition rate	570:602	the proliferation inhibition rate	570:602	The results showed that SPS2p could improve the proliferation inhibition rate; SPS2p could also elevate apoptosis rate, apoptosis index and the levels of Bax and Bak, but lower levels of Bcl-2 and FN; SPS2p could up-regulate the expression of E-cadherin mRNA, and down-regulate the expressions of N-cadherin and vimentin mRNA, and the ratio of p-AKT/AKT in HT29 cells.
28433181	3	41	theme	inhibition	588:597	arg1	rate					599:602	the proliferation inhibition rate	570:602	the proliferation inhibition rate	570:602	The results showed that SPS2p could improve the proliferation inhibition rate; SPS2p could also elevate apoptosis rate, apoptosis index and the levels of Bax and Bak, but lower levels of Bcl-2 and FN; SPS2p could up-regulate the expression of E-cadherin mRNA, and down-regulate the expressions of N-cadherin and vimentin mRNA, and the ratio of p-AKT/AKT in HT29 cells.
28433181	3	42	theme	p-AKT/AKT	870:878	arg1	expressions					808:818	the expressions	804:818	the expressions of N-cadherin and vimentin mRNA	804:850	The results showed that SPS2p could improve the proliferation inhibition rate; SPS2p could also elevate apoptosis rate, apoptosis index and the levels of Bax and Bak, but lower levels of Bcl-2 and FN; SPS2p could up-regulate the expression of E-cadherin mRNA, and down-regulate the expressions of N-cadherin and vimentin mRNA, and the ratio of p-AKT/AKT in HT29 cells.
28433181	3	42	theme	p-AKT/AKT	870:878	arg1	ratio					861:865	the ratio	857:865	the ratio of p-AKT/AKT in HT29 cells	857:892	The results showed that SPS2p could improve the proliferation inhibition rate; SPS2p could also elevate apoptosis rate, apoptosis index and the levels of Bax and Bak, but lower levels of Bcl-2 and FN; SPS2p could up-regulate the expression of E-cadherin mRNA, and down-regulate the expressions of N-cadherin and vimentin mRNA, and the ratio of p-AKT/AKT in HT29 cells.
28433181	2	43	theme	molecular	296:304	arg1	weight					306:311	The molecular weight	292:311	The molecular weight of SPS2p	292:320	The molecular weight of SPS2p showed only one molecular weight distribution (2.6×104Da) and the monosaccharide composition of SPS2p showed the presence of arabinose, mannose, glucose and galactose at the ratio of 1.31:1.00:3.59:1.59.
28433181	1	44	dep	%	238:238	arg1	carbohydrates					240:252	carbohydrates	240:252	53.6% carbohydrates	234:252	A water-soluble polysaccharide SPS2p was isolated from the whole grass of Scutellaria barbata and SPS2p contained 53.6% carbohydrates, 38.5% uronic acid and 8.2% proteins.
28433181	4	45	theme	PI3K/AKT	1064:1071	arg1	pathway					1073:1079	the PI3K/AKT pathway	1060:1079	the PI3K/AKT pathway	1060:1079	These results indicate that SPS2p can inhibit the proliferation and EMT, and promote the apoptosis in HT29 cells, which may be related to the inhibition of SPS2p on the PI3K/AKT pathway.
28433181	3	46	theme	E-cadherin	769:778	arg1	mRNA					780:783	E-cadherin mRNA	769:783	E-cadherin mRNA	769:783	The results showed that SPS2p could improve the proliferation inhibition rate; SPS2p could also elevate apoptosis rate, apoptosis index and the levels of Bax and Bak, but lower levels of Bcl-2 and FN; SPS2p could up-regulate the expression of E-cadherin mRNA, and down-regulate the expressions of N-cadherin and vimentin mRNA, and the ratio of p-AKT/AKT in HT29 cells.
28433181	4	47	from	apoptosis	984:992	arg1	cells					1002:1006	HT29 cells	997:1006	HT29 cells	997:1006	These results indicate that SPS2p can inhibit the proliferation and EMT, and promote the apoptosis in HT29 cells, which may be related to the inhibition of SPS2p on the PI3K/AKT pathway.
28433181	3	48	theme	apoptosis	630:638	arg1	rate					640:643	apoptosis rate	630:643	apoptosis rate	630:643	The results showed that SPS2p could improve the proliferation inhibition rate; SPS2p could also elevate apoptosis rate, apoptosis index and the levels of Bax and Bak, but lower levels of Bcl-2 and FN; SPS2p could up-regulate the expression of E-cadherin mRNA, and down-regulate the expressions of N-cadherin and vimentin mRNA, and the ratio of p-AKT/AKT in HT29 cells.
28433181	1	49	theme	water-soluble	122:134	arg1	SPS2p					151:155	A water-soluble polysaccharide SPS2p	120:155	A water-soluble polysaccharide SPS2p	120:155	A water-soluble polysaccharide SPS2p was isolated from the whole grass of Scutellaria barbata and SPS2p contained 53.6% carbohydrates, 38.5% uronic acid and 8.2% proteins.
28433181	3	50	theme	mRNA	780:783	arg1	expression					755:764	the expression	751:764	the expression of E-cadherin mRNA	751:783	The results showed that SPS2p could improve the proliferation inhibition rate; SPS2p could also elevate apoptosis rate, apoptosis index and the levels of Bax and Bak, but lower levels of Bcl-2 and FN; SPS2p could up-regulate the expression of E-cadherin mRNA, and down-regulate the expressions of N-cadherin and vimentin mRNA, and the ratio of p-AKT/AKT in HT29 cells.
28433181	3	51	theme	FN	723:724	arg1	levels					703:708	lower levels	697:708	lower levels of Bcl-2 and FN	697:724	The results showed that SPS2p could improve the proliferation inhibition rate; SPS2p could also elevate apoptosis rate, apoptosis index and the levels of Bax and Bak, but lower levels of Bcl-2 and FN; SPS2p could up-regulate the expression of E-cadherin mRNA, and down-regulate the expressions of N-cadherin and vimentin mRNA, and the ratio of p-AKT/AKT in HT29 cells.
28433181	0	52	theme	Scutellaria	11:21	arg1	polysaccharide					31:44	Scutellaria barbata polysaccharide	11:44	Scutellaria barbata polysaccharide	11:44	Effects of Scutellaria barbata polysaccharide on the proliferation, apoptosis and EMT of human colon cancer HT29 Cells.
28433181	1	53	theme	polysaccharide	136:149	arg1	SPS2p					151:155	A water-soluble polysaccharide SPS2p	120:155	A water-soluble polysaccharide SPS2p	120:155	A water-soluble polysaccharide SPS2p was isolated from the whole grass of Scutellaria barbata and SPS2p contained 53.6% carbohydrates, 38.5% uronic acid and 8.2% proteins.
27939510	0	0	from	antitumor	14:22	arg1	residue					86:92	soybean residue	78:92	soybean residue	78:92	Purification, antitumor and immunomodulatory activity of polysaccharides from soybean residue fermented with Morchella esculenta.
27939510	6	1	from	effect	1034:1039	arg1	cells					1051:1055	HepG-2 cells	1044:1055	HepG-2 cells	1044:1055	The results indicated that the proliferation of MP-3 on RAW 264.7 was 313.57% at 25μg/mL, which is high while MP-1 had a higher growth inhibition effect on HepG-2 cells of 68.01% at concentration of 50μg/mL.
27939510	1	2	theme	soybean	163:169	arg1	residue					171:177	soybean residue	163:177	soybean residue fermented with Morchella esculenta	163:212	Crude polysaccharides (MPS) from soybean residue fermented with Morchella esculenta were extracted and purified by DEAE Sephadex A-50 chromatography and Sephadex G-100 size-exclusion chromatography in sequence.
27939510	5	3	theme	antitumor	753:761	arg1	activities					763:772	antitumor activities	753:772	antitumor activities of the human hepatocellular cell line (HepG-2)	753:819	Furthermore, the influence of polysaccharides fractions upon activation of macrophage cells (RAW 264.7), antitumor activities of the human hepatocellular cell line (HepG-2) and human cervical carcinoma cells (Hela) in vitro were evaluated.
27939510	5	3	theme	antitumor	753:761	arg1	HepG-2					813:818	HepG-2	813:818	HepG-2	813:818	Furthermore, the influence of polysaccharides fractions upon activation of macrophage cells (RAW 264.7), antitumor activities of the human hepatocellular cell line (HepG-2) and human cervical carcinoma cells (Hela) in vitro were evaluated.
27939510	6	4	theme	growth	1016:1021	arg1	effect					1034:1039	a higher growth inhibition effect	1007:1039	a higher growth inhibition effect on HepG-2 cells of 68.01%	1007:1065	The results indicated that the proliferation of MP-3 on RAW 264.7 was 313.57% at 25μg/mL, which is high while MP-1 had a higher growth inhibition effect on HepG-2 cells of 68.01% at concentration of 50μg/mL.
27939510	8	5	theme	pharmacological	1428:1442	arg1	fields					1444:1449	pharmacological fields	1428:1449	pharmacological fields	1428:1449	These findings suggest that the purified polysaccharides fractions may be a potent candidate for human hepatocellular and cervical carcinoma treatment and prevention in functional foods and pharmacological fields.
27939510	4	6	theme	ultraviolet	562:572	arg1	spectrum					574:581	ultraviolet spectrum	562:581	ultraviolet spectrum	562:581	The monosaccharide composition, ultraviolet spectrum, infrared spectrum and NMR of the three fractions were analyzed.
27939510	0	7	theme	Morchella	109:117	arg1	esculenta					119:127	Morchella esculenta	109:127	Morchella esculenta	109:127	Purification, antitumor and immunomodulatory activity of polysaccharides from soybean residue fermented with Morchella esculenta.
27939510	0	8	from	Purification	0:11	arg1	residue					86:92	soybean residue	78:92	soybean residue	78:92	Purification, antitumor and immunomodulatory activity of polysaccharides from soybean residue fermented with Morchella esculenta.
27939510	1	9	from	residue	171:177	arg1	MPS					153:155	MPS	153:155	MPS	153:155	Crude polysaccharides (MPS) from soybean residue fermented with Morchella esculenta were extracted and purified by DEAE Sephadex A-50 chromatography and Sephadex G-100 size-exclusion chromatography in sequence.
27939510	1	9	from	residue	171:177	arg1	polysaccharides					136:150	Crude polysaccharides	130:150	Crude polysaccharides (MPS) from soybean residue fermented with Morchella esculenta	130:212	Crude polysaccharides (MPS) from soybean residue fermented with Morchella esculenta were extracted and purified by DEAE Sephadex A-50 chromatography and Sephadex G-100 size-exclusion chromatography in sequence.
27939510	8	10	theme	polysaccharides	1279:1293	arg1	candidate					1321:1329	a potent candidate	1312:1329	a potent candidate for human hepatocellular and cervical carcinoma treatment and prevention	1312:1402	These findings suggest that the purified polysaccharides fractions may be a potent candidate for human hepatocellular and cervical carcinoma treatment and prevention in functional foods and pharmacological fields.
27939510	8	10	theme	polysaccharides	1279:1293	arg1	fractions					1295:1303	the purified polysaccharides fractions	1266:1303	the purified polysaccharides fractions	1266:1303	These findings suggest that the purified polysaccharides fractions may be a potent candidate for human hepatocellular and cervical carcinoma treatment and prevention in functional foods and pharmacological fields.
27939510	1	11	theme	Sephadex	283:290	arg1	chromatography					313:326	Sephadex G-100 size-exclusion chromatography	283:326	Sephadex G-100 size-exclusion chromatography	283:326	Crude polysaccharides (MPS) from soybean residue fermented with Morchella esculenta were extracted and purified by DEAE Sephadex A-50 chromatography and Sephadex G-100 size-exclusion chromatography in sequence.
27939510	5	12	theme	cells	734:738	arg1	activation					709:718	activation	709:718	activation of macrophage cells (RAW 264.7), antitumor activities of the human hepatocellular cell line (HepG-2) and human cervical carcinoma cells (Hela) in vitro	709:870	Furthermore, the influence of polysaccharides fractions upon activation of macrophage cells (RAW 264.7), antitumor activities of the human hepatocellular cell line (HepG-2) and human cervical carcinoma cells (Hela) in vitro were evaluated.
27939510	6	13	from	25μg/mL	969:975	arg1	proliferation					919:931	the proliferation	915:931	the proliferation of MP-3 on RAW 264.7	915:952	The results indicated that the proliferation of MP-3 on RAW 264.7 was 313.57% at 25μg/mL, which is high while MP-1 had a higher growth inhibition effect on HepG-2 cells of 68.01% at concentration of 50μg/mL.
27939510	6	13	from	25μg/mL	969:975	arg1	%					964:964	313.57%	958:964	313.57%	958:964	The results indicated that the proliferation of MP-3 on RAW 264.7 was 313.57% at 25μg/mL, which is high while MP-1 had a higher growth inhibition effect on HepG-2 cells of 68.01% at concentration of 50μg/mL.
27939510	7	14	theme	HepG-2	1154:1159	arg1	cells					1161:1165	HepG-2 cells	1154:1165	HepG-2 cells	1154:1165	The fractions of MP-1, MP-3 and MP-4 induced apoptosis in HepG-2 cells and Hela cells by arresting cell cycle progression at the G0/G1 phase.
27939510	6	15	theme	inhibition	1023:1032	arg1	effect					1034:1039	a higher growth inhibition effect	1007:1039	a higher growth inhibition effect on HepG-2 cells of 68.01%	1007:1065	The results indicated that the proliferation of MP-3 on RAW 264.7 was 313.57% at 25μg/mL, which is high while MP-1 had a higher growth inhibition effect on HepG-2 cells of 68.01% at concentration of 50μg/mL.
27939510	1	16	theme	G-100	292:296	arg1	chromatography					313:326	Sephadex G-100 size-exclusion chromatography	283:326	Sephadex G-100 size-exclusion chromatography	283:326	Crude polysaccharides (MPS) from soybean residue fermented with Morchella esculenta were extracted and purified by DEAE Sephadex A-50 chromatography and Sephadex G-100 size-exclusion chromatography in sequence.
27939510	6	17	theme	higher	1009:1014	arg1	effect					1034:1039	a higher growth inhibition effect	1007:1039	a higher growth inhibition effect on HepG-2 cells of 68.01%	1007:1065	The results indicated that the proliferation of MP-3 on RAW 264.7 was 313.57% at 25μg/mL, which is high while MP-1 had a higher growth inhibition effect on HepG-2 cells of 68.01% at concentration of 50μg/mL.
27939510	2	18	theme	purification	407:418	arg1	steps					420:424	the purification steps	403:424	the purification steps	403:424	Three main fractions MP-1, MP-3 and MP-4 were obtained during the purification steps.
27939510	5	19	theme	cervical	831:838	arg1	Hela					857:860	Hela	857:860	Hela	857:860	Furthermore, the influence of polysaccharides fractions upon activation of macrophage cells (RAW 264.7), antitumor activities of the human hepatocellular cell line (HepG-2) and human cervical carcinoma cells (Hela) in vitro were evaluated.
27939510	5	19	theme	cervical	831:838	arg1	cells					850:854	human cervical carcinoma cells	825:854	human cervical carcinoma cells (Hela)	825:861	Furthermore, the influence of polysaccharides fractions upon activation of macrophage cells (RAW 264.7), antitumor activities of the human hepatocellular cell line (HepG-2) and human cervical carcinoma cells (Hela) in vitro were evaluated.
27939510	5	20	theme	human	781:785	arg1	line					807:810	the human hepatocellular cell line	777:810	the human hepatocellular cell line	777:810	Furthermore, the influence of polysaccharides fractions upon activation of macrophage cells (RAW 264.7), antitumor activities of the human hepatocellular cell line (HepG-2) and human cervical carcinoma cells (Hela) in vitro were evaluated.
27939510	0	21	from	activity	45:52	arg1	residue					86:92	soybean residue	78:92	soybean residue	78:92	Purification, antitumor and immunomodulatory activity of polysaccharides from soybean residue fermented with Morchella esculenta.
27939510	6	22	from	RAW	944:946	arg1	%					964:964	313.57%	958:964	313.57%	958:964	The results indicated that the proliferation of MP-3 on RAW 264.7 was 313.57% at 25μg/mL, which is high while MP-1 had a higher growth inhibition effect on HepG-2 cells of 68.01% at concentration of 50μg/mL.
27939510	6	22	from	RAW	944:946	arg1	proliferation					919:931	the proliferation	915:931	the proliferation of MP-3 on RAW 264.7	915:952	The results indicated that the proliferation of MP-3 on RAW 264.7 was 313.57% at 25μg/mL, which is high while MP-1 had a higher growth inhibition effect on HepG-2 cells of 68.01% at concentration of 50μg/mL.
27939510	6	23	theme	50μg/mL	1087:1093	arg1	concentration					1070:1082	concentration	1070:1082	concentration of 50μg/mL	1070:1093	The results indicated that the proliferation of MP-3 on RAW 264.7 was 313.57% at 25μg/mL, which is high while MP-1 had a higher growth inhibition effect on HepG-2 cells of 68.01% at concentration of 50μg/mL.
27939510	5	24	theme	carcinoma	840:848	arg1	Hela					857:860	Hela	857:860	Hela	857:860	Furthermore, the influence of polysaccharides fractions upon activation of macrophage cells (RAW 264.7), antitumor activities of the human hepatocellular cell line (HepG-2) and human cervical carcinoma cells (Hela) in vitro were evaluated.
27939510	5	24	theme	carcinoma	840:848	arg1	cells					850:854	human cervical carcinoma cells	825:854	human cervical carcinoma cells (Hela)	825:861	Furthermore, the influence of polysaccharides fractions upon activation of macrophage cells (RAW 264.7), antitumor activities of the human hepatocellular cell line (HepG-2) and human cervical carcinoma cells (Hela) in vitro were evaluated.
27939510	5	25	theme	hepatocellular	787:800	arg1	line					807:810	the human hepatocellular cell line	777:810	the human hepatocellular cell line	777:810	Furthermore, the influence of polysaccharides fractions upon activation of macrophage cells (RAW 264.7), antitumor activities of the human hepatocellular cell line (HepG-2) and human cervical carcinoma cells (Hela) in vitro were evaluated.
27939510	7	26	theme	MP-4	1128:1131	arg1	fractions					1100:1108	The fractions	1096:1108	The fractions of MP-1, MP-3 and MP-4	1096:1131	The fractions of MP-1, MP-3 and MP-4 induced apoptosis in HepG-2 cells and Hela cells by arresting cell cycle progression at the G0/G1 phase.
27939510	4	27	theme	monosaccharide	534:547	arg1	composition					549:559	monosaccharide composition	534:559	monosaccharide composition	534:559	The monosaccharide composition, ultraviolet spectrum, infrared spectrum and NMR of the three fractions were analyzed.
27939510	8	28	theme	purified	1270:1277	arg1	candidate					1321:1329	a potent candidate	1312:1329	a potent candidate for human hepatocellular and cervical carcinoma treatment and prevention	1312:1402	These findings suggest that the purified polysaccharides fractions may be a potent candidate for human hepatocellular and cervical carcinoma treatment and prevention in functional foods and pharmacological fields.
27939510	8	28	theme	purified	1270:1277	arg1	fractions					1295:1303	the purified polysaccharides fractions	1266:1303	the purified polysaccharides fractions	1266:1303	These findings suggest that the purified polysaccharides fractions may be a potent candidate for human hepatocellular and cervical carcinoma treatment and prevention in functional foods and pharmacological fields.
27939510	1	29	theme	Morchella	194:202	arg1	esculenta					204:212	Morchella esculenta	194:212	Morchella esculenta	194:212	Crude polysaccharides (MPS) from soybean residue fermented with Morchella esculenta were extracted and purified by DEAE Sephadex A-50 chromatography and Sephadex G-100 size-exclusion chromatography in sequence.
27939510	5	30	theme	cell	802:805	arg1	line					807:810	the human hepatocellular cell line	777:810	the human hepatocellular cell line	777:810	Furthermore, the influence of polysaccharides fractions upon activation of macrophage cells (RAW 264.7), antitumor activities of the human hepatocellular cell line (HepG-2) and human cervical carcinoma cells (Hela) in vitro were evaluated.
27939510	1	31	theme	size-exclusion	298:311	arg1	chromatography					313:326	Sephadex G-100 size-exclusion chromatography	283:326	Sephadex G-100 size-exclusion chromatography	283:326	Crude polysaccharides (MPS) from soybean residue fermented with Morchella esculenta were extracted and purified by DEAE Sephadex A-50 chromatography and Sephadex G-100 size-exclusion chromatography in sequence.
27939510	2	32	theme	main	347:350	arg1	MP-3					368:371	MP-3	368:371	MP-3	368:371	Three main fractions MP-1, MP-3 and MP-4 were obtained during the purification steps.
27939510	2	32	theme	main	347:350	arg1	MP-1					362:365	MP-1	362:365	MP-1	362:365	Three main fractions MP-1, MP-3 and MP-4 were obtained during the purification steps.
27939510	2	32	theme	main	347:350	arg1	MP-4					377:380	MP-4	377:380	MP-4	377:380	Three main fractions MP-1, MP-3 and MP-4 were obtained during the purification steps.
27939510	2	32	theme	main	347:350	arg1	fractions					352:360	Three main fractions MP-1, MP-3 and MP-4	341:380	Three main fractions MP-1, MP-3 and MP-4	341:380	Three main fractions MP-1, MP-3 and MP-4 were obtained during the purification steps.
27939510	7	33	theme	G0/G1	1225:1229	arg1	phase					1231:1235	the G0/G1 phase	1221:1235	the G0/G1 phase	1221:1235	The fractions of MP-1, MP-3 and MP-4 induced apoptosis in HepG-2 cells and Hela cells by arresting cell cycle progression at the G0/G1 phase.
27939510	5	34	theme	line	807:810	arg1	HepG-2					813:818	HepG-2	813:818	HepG-2	813:818	Furthermore, the influence of polysaccharides fractions upon activation of macrophage cells (RAW 264.7), antitumor activities of the human hepatocellular cell line (HepG-2) and human cervical carcinoma cells (Hela) in vitro were evaluated.
27939510	5	34	theme	line	807:810	arg1	Hela					857:860	Hela	857:860	Hela	857:860	Furthermore, the influence of polysaccharides fractions upon activation of macrophage cells (RAW 264.7), antitumor activities of the human hepatocellular cell line (HepG-2) and human cervical carcinoma cells (Hela) in vitro were evaluated.
27939510	5	34	theme	line	807:810	arg1	cells					734:738	macrophage cells	723:738	macrophage cells (RAW 264.7)	723:750	Furthermore, the influence of polysaccharides fractions upon activation of macrophage cells (RAW 264.7), antitumor activities of the human hepatocellular cell line (HepG-2) and human cervical carcinoma cells (Hela) in vitro were evaluated.
27939510	5	34	theme	line	807:810	arg1	activities					763:772	antitumor activities	753:772	antitumor activities of the human hepatocellular cell line (HepG-2)	753:819	Furthermore, the influence of polysaccharides fractions upon activation of macrophage cells (RAW 264.7), antitumor activities of the human hepatocellular cell line (HepG-2) and human cervical carcinoma cells (Hela) in vitro were evaluated.
27939510	5	34	theme	line	807:810	arg1	RAW					741:743	RAW 264.7	741:749	RAW 264.7	741:749	Furthermore, the influence of polysaccharides fractions upon activation of macrophage cells (RAW 264.7), antitumor activities of the human hepatocellular cell line (HepG-2) and human cervical carcinoma cells (Hela) in vitro were evaluated.
27939510	5	34	theme	line	807:810	arg1	cells					850:854	human cervical carcinoma cells	825:854	human cervical carcinoma cells (Hela)	825:861	Furthermore, the influence of polysaccharides fractions upon activation of macrophage cells (RAW 264.7), antitumor activities of the human hepatocellular cell line (HepG-2) and human cervical carcinoma cells (Hela) in vitro were evaluated.
27939510	6	35	theme	MP-3	936:939	arg1	%					964:964	313.57%	958:964	313.57%	958:964	The results indicated that the proliferation of MP-3 on RAW 264.7 was 313.57% at 25μg/mL, which is high while MP-1 had a higher growth inhibition effect on HepG-2 cells of 68.01% at concentration of 50μg/mL.
27939510	6	35	theme	MP-3	936:939	arg1	proliferation					919:931	the proliferation	915:931	the proliferation of MP-3 on RAW 264.7	915:952	The results indicated that the proliferation of MP-3 on RAW 264.7 was 313.57% at 25μg/mL, which is high while MP-1 had a higher growth inhibition effect on HepG-2 cells of 68.01% at concentration of 50μg/mL.
27939510	5	36	theme	fractions	694:702	arg1	influence					665:673	the influence	661:673	the influence of polysaccharides fractions upon activation of macrophage cells (RAW 264.7), antitumor activities of the human hepatocellular cell line (HepG-2) and human cervical carcinoma cells (Hela) in vitro	661:870	Furthermore, the influence of polysaccharides fractions upon activation of macrophage cells (RAW 264.7), antitumor activities of the human hepatocellular cell line (HepG-2) and human cervical carcinoma cells (Hela) in vitro were evaluated.
27939510	8	37	dep	carcinoma	1369:1377	arg1	treatment					1379:1387	treatment	1379:1387	treatment	1379:1387	These findings suggest that the purified polysaccharides fractions may be a potent candidate for human hepatocellular and cervical carcinoma treatment and prevention in functional foods and pharmacological fields.
27939510	6	38	from	proliferation	919:931	arg1	RAW					944:946	RAW 264.7	944:952	RAW 264.7	944:952	The results indicated that the proliferation of MP-3 on RAW 264.7 was 313.57% at 25μg/mL, which is high while MP-1 had a higher growth inhibition effect on HepG-2 cells of 68.01% at concentration of 50μg/mL.
27939510	6	39	theme	%	1065:1065	arg1	effect					1034:1039	a higher growth inhibition effect	1007:1039	a higher growth inhibition effect on HepG-2 cells of 68.01%	1007:1065	The results indicated that the proliferation of MP-3 on RAW 264.7 was 313.57% at 25μg/mL, which is high while MP-1 had a higher growth inhibition effect on HepG-2 cells of 68.01% at concentration of 50μg/mL.
27939510	5	40	theme	activities	763:772	arg1	activation					709:718	activation	709:718	activation of macrophage cells (RAW 264.7), antitumor activities of the human hepatocellular cell line (HepG-2) and human cervical carcinoma cells (Hela) in vitro	709:870	Furthermore, the influence of polysaccharides fractions upon activation of macrophage cells (RAW 264.7), antitumor activities of the human hepatocellular cell line (HepG-2) and human cervical carcinoma cells (Hela) in vitro were evaluated.
27939510	7	41	theme	cycle	1200:1204	arg1	progression					1206:1216	cell cycle progression	1195:1216	cell cycle progression	1195:1216	The fractions of MP-1, MP-3 and MP-4 induced apoptosis in HepG-2 cells and Hela cells by arresting cell cycle progression at the G0/G1 phase.
27939510	0	42	from	residue	86:92	arg1	antitumor					14:22	antitumor	14:22	antitumor	14:22	Purification, antitumor and immunomodulatory activity of polysaccharides from soybean residue fermented with Morchella esculenta.
27939510	0	42	from	residue	86:92	arg1	activity					45:52	immunomodulatory activity	28:52	immunomodulatory activity	28:52	Purification, antitumor and immunomodulatory activity of polysaccharides from soybean residue fermented with Morchella esculenta.
27939510	0	42	from	residue	86:92	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification, antitumor and immunomodulatory activity of polysaccharides from soybean residue fermented with Morchella esculenta.
27939510	0	42	from	residue	86:92	arg1	polysaccharides					57:71	polysaccharides	57:71	polysaccharides from soybean residue	57:92	Purification, antitumor and immunomodulatory activity of polysaccharides from soybean residue fermented with Morchella esculenta.
27939510	8	43	from	candidate	1321:1329	arg1	foods					1418:1422	functional foods	1407:1422	functional foods	1407:1422	These findings suggest that the purified polysaccharides fractions may be a potent candidate for human hepatocellular and cervical carcinoma treatment and prevention in functional foods and pharmacological fields.
27939510	8	43	from	candidate	1321:1329	arg1	fields					1444:1449	pharmacological fields	1428:1449	pharmacological fields	1428:1449	These findings suggest that the purified polysaccharides fractions may be a potent candidate for human hepatocellular and cervical carcinoma treatment and prevention in functional foods and pharmacological fields.
27939510	7	44	theme	cell	1195:1198	arg1	progression					1206:1216	cell cycle progression	1195:1216	cell cycle progression	1195:1216	The fractions of MP-1, MP-3 and MP-4 induced apoptosis in HepG-2 cells and Hela cells by arresting cell cycle progression at the G0/G1 phase.
27939510	0	45	theme	immunomodulatory	28:43	arg1	activity					45:52	immunomodulatory activity	28:52	immunomodulatory activity	28:52	Purification, antitumor and immunomodulatory activity of polysaccharides from soybean residue fermented with Morchella esculenta.
27939510	5	46	theme	cells	850:854	arg1	activation					709:718	activation	709:718	activation of macrophage cells (RAW 264.7), antitumor activities of the human hepatocellular cell line (HepG-2) and human cervical carcinoma cells (Hela) in vitro	709:870	Furthermore, the influence of polysaccharides fractions upon activation of macrophage cells (RAW 264.7), antitumor activities of the human hepatocellular cell line (HepG-2) and human cervical carcinoma cells (Hela) in vitro were evaluated.
27939510	6	47	from	MP-3	936:939	arg1	RAW					944:946	RAW 264.7	944:952	RAW 264.7	944:952	The results indicated that the proliferation of MP-3 on RAW 264.7 was 313.57% at 25μg/mL, which is high while MP-1 had a higher growth inhibition effect on HepG-2 cells of 68.01% at concentration of 50μg/mL.
27939510	4	48	theme	fractions	623:631	arg1	NMR					606:608	NMR	606:608	NMR	606:608	The monosaccharide composition, ultraviolet spectrum, infrared spectrum and NMR of the three fractions were analyzed.
27939510	4	48	theme	fractions	623:631	arg1	composition					549:559	monosaccharide composition	534:559	monosaccharide composition	534:559	The monosaccharide composition, ultraviolet spectrum, infrared spectrum and NMR of the three fractions were analyzed.
27939510	4	48	theme	fractions	623:631	arg1	spectrum					574:581	ultraviolet spectrum	562:581	ultraviolet spectrum	562:581	The monosaccharide composition, ultraviolet spectrum, infrared spectrum and NMR of the three fractions were analyzed.
27939510	4	48	theme	fractions	623:631	arg1	spectrum					593:600	infrared spectrum	584:600	infrared spectrum	584:600	The monosaccharide composition, ultraviolet spectrum, infrared spectrum and NMR of the three fractions were analyzed.
27939510	0	49	theme	polysaccharides	57:71	arg1	antitumor					14:22	antitumor	14:22	antitumor	14:22	Purification, antitumor and immunomodulatory activity of polysaccharides from soybean residue fermented with Morchella esculenta.
27939510	0	49	theme	polysaccharides	57:71	arg1	activity					45:52	immunomodulatory activity	28:52	immunomodulatory activity	28:52	Purification, antitumor and immunomodulatory activity of polysaccharides from soybean residue fermented with Morchella esculenta.
27939510	0	49	theme	polysaccharides	57:71	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification, antitumor and immunomodulatory activity of polysaccharides from soybean residue fermented with Morchella esculenta.
27939510	5	50	theme	macrophage	723:732	arg1	RAW					741:743	RAW 264.7	741:749	RAW 264.7	741:749	Furthermore, the influence of polysaccharides fractions upon activation of macrophage cells (RAW 264.7), antitumor activities of the human hepatocellular cell line (HepG-2) and human cervical carcinoma cells (Hela) in vitro were evaluated.
27939510	5	50	theme	macrophage	723:732	arg1	cells					734:738	macrophage cells	723:738	macrophage cells (RAW 264.7)	723:750	Furthermore, the influence of polysaccharides fractions upon activation of macrophage cells (RAW 264.7), antitumor activities of the human hepatocellular cell line (HepG-2) and human cervical carcinoma cells (Hela) in vitro were evaluated.
27939510	6	51	contain	had	1003:1005	arg1	MP-1					998:1001	MP-1	998:1001	MP-1	998:1001	The results indicated that the proliferation of MP-3 on RAW 264.7 was 313.57% at 25μg/mL, which is high while MP-1 had a higher growth inhibition effect on HepG-2 cells of 68.01% at concentration of 50μg/mL.
27939510	6	51	contain	had	1003:1005	arg2	effect					1034:1039	a higher growth inhibition effect	1007:1039	a higher growth inhibition effect on HepG-2 cells of 68.01%	1007:1065	The results indicated that the proliferation of MP-3 on RAW 264.7 was 313.57% at 25μg/mL, which is high while MP-1 had a higher growth inhibition effect on HepG-2 cells of 68.01% at concentration of 50μg/mL.
27939510	1	52	theme	Crude	130:134	arg1	MPS					153:155	MPS	153:155	MPS	153:155	Crude polysaccharides (MPS) from soybean residue fermented with Morchella esculenta were extracted and purified by DEAE Sephadex A-50 chromatography and Sephadex G-100 size-exclusion chromatography in sequence.
27939510	1	52	theme	Crude	130:134	arg1	polysaccharides					136:150	Crude polysaccharides	130:150	Crude polysaccharides (MPS) from soybean residue fermented with Morchella esculenta	130:212	Crude polysaccharides (MPS) from soybean residue fermented with Morchella esculenta were extracted and purified by DEAE Sephadex A-50 chromatography and Sephadex G-100 size-exclusion chromatography in sequence.
27939510	3	53	theme	recovery	431:438	arg1	%					473:473	26.2%	469:473	26.2%	469:473	The recovery rates based on MPS used were 26.2%, 29.1% and 18.7% for MP-1, MP-3 and MP-4 respectively.
27939510	3	53	theme	recovery	431:438	arg1	rates					440:444	The recovery rates	427:444	The recovery rates based on MPS used	427:462	The recovery rates based on MPS used were 26.2%, 29.1% and 18.7% for MP-1, MP-3 and MP-4 respectively.
27939510	8	54	theme	potent	1314:1319	arg1	candidate					1321:1329	a potent candidate	1312:1329	a potent candidate for human hepatocellular and cervical carcinoma treatment and prevention	1312:1402	These findings suggest that the purified polysaccharides fractions may be a potent candidate for human hepatocellular and cervical carcinoma treatment and prevention in functional foods and pharmacological fields.
27939510	8	54	theme	potent	1314:1319	arg1	fractions					1295:1303	the purified polysaccharides fractions	1266:1303	the purified polysaccharides fractions	1266:1303	These findings suggest that the purified polysaccharides fractions may be a potent candidate for human hepatocellular and cervical carcinoma treatment and prevention in functional foods and pharmacological fields.
27939510	7	55	theme	MP-1	1113:1116	arg1	fractions					1100:1108	The fractions	1096:1108	The fractions of MP-1, MP-3 and MP-4	1096:1131	The fractions of MP-1, MP-3 and MP-4 induced apoptosis in HepG-2 cells and Hela cells by arresting cell cycle progression at the G0/G1 phase.
27939510	7	56	theme	MP-3	1119:1122	arg1	fractions					1100:1108	The fractions	1096:1108	The fractions of MP-1, MP-3 and MP-4	1096:1131	The fractions of MP-1, MP-3 and MP-4 induced apoptosis in HepG-2 cells and Hela cells by arresting cell cycle progression at the G0/G1 phase.
27939510	2	57	dep	fractions	352:360	arg1	MP-3					368:371	MP-3	368:371	MP-3	368:371	Three main fractions MP-1, MP-3 and MP-4 were obtained during the purification steps.
27939510	2	57	dep	fractions	352:360	arg1	MP-1					362:365	MP-1	362:365	MP-1	362:365	Three main fractions MP-1, MP-3 and MP-4 were obtained during the purification steps.
27939510	2	57	dep	fractions	352:360	arg1	MP-4					377:380	MP-4	377:380	MP-4	377:380	Three main fractions MP-1, MP-3 and MP-4 were obtained during the purification steps.
27939510	2	57	dep	fractions	352:360	arg1	fractions					352:360	Three main fractions MP-1, MP-3 and MP-4	341:380	Three main fractions MP-1, MP-3 and MP-4	341:380	Three main fractions MP-1, MP-3 and MP-4 were obtained during the purification steps.
27939510	5	58	theme	polysaccharides	678:692	arg1	fractions					694:702	polysaccharides fractions	678:702	polysaccharides fractions	678:702	Furthermore, the influence of polysaccharides fractions upon activation of macrophage cells (RAW 264.7), antitumor activities of the human hepatocellular cell line (HepG-2) and human cervical carcinoma cells (Hela) in vitro were evaluated.
27939510	8	59	theme	functional	1407:1416	arg1	foods					1418:1422	functional foods	1407:1422	functional foods	1407:1422	These findings suggest that the purified polysaccharides fractions may be a potent candidate for human hepatocellular and cervical carcinoma treatment and prevention in functional foods and pharmacological fields.
27939510	6	60	theme	HepG-2	1044:1049	arg1	cells					1051:1055	HepG-2 cells	1044:1055	HepG-2 cells	1044:1055	The results indicated that the proliferation of MP-3 on RAW 264.7 was 313.57% at 25μg/mL, which is high while MP-1 had a higher growth inhibition effect on HepG-2 cells of 68.01% at concentration of 50μg/mL.
27939510	1	61	theme	DEAE	245:248	arg1	chromatography					264:277	DEAE Sephadex A-50 chromatography	245:277	DEAE Sephadex A-50 chromatography	245:277	Crude polysaccharides (MPS) from soybean residue fermented with Morchella esculenta were extracted and purified by DEAE Sephadex A-50 chromatography and Sephadex G-100 size-exclusion chromatography in sequence.
27939510	0	62	theme	soybean	78:84	arg1	residue					86:92	soybean residue	78:92	soybean residue	78:92	Purification, antitumor and immunomodulatory activity of polysaccharides from soybean residue fermented with Morchella esculenta.
27939510	5	63	theme	human	825:829	arg1	Hela					857:860	Hela	857:860	Hela	857:860	Furthermore, the influence of polysaccharides fractions upon activation of macrophage cells (RAW 264.7), antitumor activities of the human hepatocellular cell line (HepG-2) and human cervical carcinoma cells (Hela) in vitro were evaluated.
27939510	5	63	theme	human	825:829	arg1	cells					850:854	human cervical carcinoma cells	825:854	human cervical carcinoma cells (Hela)	825:861	Furthermore, the influence of polysaccharides fractions upon activation of macrophage cells (RAW 264.7), antitumor activities of the human hepatocellular cell line (HepG-2) and human cervical carcinoma cells (Hela) in vitro were evaluated.
27939510	8	64	theme	hepatocellular	1341:1354	arg1	carcinoma					1369:1377	human hepatocellular and cervical carcinoma treatment and prevention	1335:1402	carcinoma	1369:1377	These findings suggest that the purified polysaccharides fractions may be a potent candidate for human hepatocellular and cervical carcinoma treatment and prevention in functional foods and pharmacological fields.
27939510	1	65	theme	Sephadex	250:257	arg1	chromatography					264:277	DEAE Sephadex A-50 chromatography	245:277	DEAE Sephadex A-50 chromatography	245:277	Crude polysaccharides (MPS) from soybean residue fermented with Morchella esculenta were extracted and purified by DEAE Sephadex A-50 chromatography and Sephadex G-100 size-exclusion chromatography in sequence.
27939510	4	66	theme	infrared	584:591	arg1	spectrum					593:600	infrared spectrum	584:600	infrared spectrum	584:600	The monosaccharide composition, ultraviolet spectrum, infrared spectrum and NMR of the three fractions were analyzed.
27939510	8	67	theme	human	1335:1339	arg1	carcinoma					1369:1377	human hepatocellular and cervical carcinoma treatment and prevention	1335:1402	carcinoma	1369:1377	These findings suggest that the purified polysaccharides fractions may be a potent candidate for human hepatocellular and cervical carcinoma treatment and prevention in functional foods and pharmacological fields.
27939510	7	68	theme	Hela	1171:1174	arg1	cells					1176:1180	Hela cells	1171:1180	Hela cells	1171:1180	The fractions of MP-1, MP-3 and MP-4 induced apoptosis in HepG-2 cells and Hela cells by arresting cell cycle progression at the G0/G1 phase.
27939510	1	69	theme	A-50	259:262	arg1	chromatography					264:277	DEAE Sephadex A-50 chromatography	245:277	DEAE Sephadex A-50 chromatography	245:277	Crude polysaccharides (MPS) from soybean residue fermented with Morchella esculenta were extracted and purified by DEAE Sephadex A-50 chromatography and Sephadex G-100 size-exclusion chromatography in sequence.
24299744	0	0	theme	soil	82:85	arg1	conditions					87:96	indoor soil conditions	75:96	indoor soil conditions	75:96	Degradation behavior of biocomposites based on cassava starch buried under indoor soil conditions.
24299744	5	1	theme	patterns	742:749	arg1	formation					729:737	the formation	725:737	the formation of patterns and cracks on the surface of the materials aged in the soils	725:810	Scanning electron microscopy (SEM) analysis showed the formation of patterns and cracks on the surface of the materials aged in the soils.
24299744	1	2	theme	composite	145:153	arg1	films					155:159	cassava (tapioca) starch based composite films	114:159	cassava (tapioca) starch based composite films	114:159	Degradation of cassava (tapioca) starch based composite films during indoor soil burial experiments was analyzed using five factors, three levels Box-Behnken response surface design.
24299744	0	3	theme	indoor	75:80	arg1	conditions					87:96	indoor soil conditions	75:96	indoor soil conditions	75:96	Degradation behavior of biocomposites based on cassava starch buried under indoor soil conditions.
24299744	3	4	from	reduction	465:473	arg1	weight					478:483	weight	478:483	weight	478:483	The reduction in weight and mechanical property was associated with preferential loss of matrix components of the films.
24299744	3	4	from	reduction	465:473	arg1	property					500:507	mechanical property	489:507	mechanical property	489:507	The reduction in weight and mechanical property was associated with preferential loss of matrix components of the films.
24299744	1	5	theme	films	155:159	arg1	Degradation					99:109	Degradation	99:109	Degradation of cassava (tapioca) starch based composite films during indoor soil burial experiments	99:197	Degradation of cassava (tapioca) starch based composite films during indoor soil burial experiments was analyzed using five factors, three levels Box-Behnken response surface design.
24299744	1	6	theme	Box-Behnken	245:255	arg1	design					274:279	five factors, three levels Box-Behnken response surface design	218:279	five factors, three levels Box-Behnken response surface design	218:279	Degradation of cassava (tapioca) starch based composite films during indoor soil burial experiments was analyzed using five factors, three levels Box-Behnken response surface design.
24299744	2	7	theme	water	332:336	arg1	sorption					338:345	increased water sorption	322:345	increased water sorption	322:345	From the results, it was observed that, increased water sorption promotes the entry of soil microorganism and it utilizes the starch films as a source of energy for their growth.
24299744	3	8	theme	preferential	529:540	arg1	loss					542:545	preferential loss	529:545	preferential loss of matrix components of the films	529:579	The reduction in weight and mechanical property was associated with preferential loss of matrix components of the films.
24299744	1	9	theme	response	257:264	arg1	design					274:279	five factors, three levels Box-Behnken response surface design	218:279	five factors, three levels Box-Behnken response surface design	218:279	Degradation of cassava (tapioca) starch based composite films during indoor soil burial experiments was analyzed using five factors, three levels Box-Behnken response surface design.
24299744	1	10	theme	surface	266:272	arg1	design					274:279	five factors, three levels Box-Behnken response surface design	218:279	five factors, three levels Box-Behnken response surface design	218:279	Degradation of cassava (tapioca) starch based composite films during indoor soil burial experiments was analyzed using five factors, three levels Box-Behnken response surface design.
24299744	5	11	from	aged	794:797	arg1	soils					806:810	the soils	802:810	the soils	802:810	Scanning electron microscopy (SEM) analysis showed the formation of patterns and cracks on the surface of the materials aged in the soils.
24299744	3	12	theme	mechanical	489:498	arg1	property					500:507	mechanical property	489:507	mechanical property	489:507	The reduction in weight and mechanical property was associated with preferential loss of matrix components of the films.
24299744	2	13	theme	microorganism	374:386	arg1	entry					360:364	the entry	356:364	the entry of soil microorganism	356:386	From the results, it was observed that, increased water sorption promotes the entry of soil microorganism and it utilizes the starch films as a source of energy for their growth.
24299744	6	14	theme	order	838:842	arg1	models					855:860	second order polynomial models	831:860	second order polynomial models	831:860	From the results, second order polynomial models were developed for the responses.
24299744	2	15	theme	soil	369:372	arg1	microorganism					374:386	soil microorganism	369:386	soil microorganism	369:386	From the results, it was observed that, increased water sorption promotes the entry of soil microorganism and it utilizes the starch films as a source of energy for their growth.
24299744	6	16	theme	second	831:836	arg1	models					855:860	second order polynomial models	831:860	second order polynomial models	831:860	From the results, second order polynomial models were developed for the responses.
24299744	6	17	theme	polynomial	844:853	arg1	models					855:860	second order polynomial models	831:860	second order polynomial models	831:860	From the results, second order polynomial models were developed for the responses.
24299744	1	18	theme	soil	175:178	arg1	experiments					187:197	indoor soil burial experiments	168:197	indoor soil burial experiments	168:197	Degradation of cassava (tapioca) starch based composite films during indoor soil burial experiments was analyzed using five factors, three levels Box-Behnken response surface design.
24299744	0	19	theme	Degradation	0:10	arg1	behavior					12:19	Degradation behavior	0:19	Degradation behavior of biocomposites based on cassava starch	0:60	Degradation behavior of biocomposites based on cassava starch buried under indoor soil conditions.
24299744	4	20	theme	films	636:640	arg1	degradation					621:631	the degradation	617:631	the degradation of films	617:640	The microorganisms associated with the degradation of films were quantified and identified.
24299744	1	21	theme	burial	180:185	arg1	experiments					187:197	indoor soil burial experiments	168:197	indoor soil burial experiments	168:197	Degradation of cassava (tapioca) starch based composite films during indoor soil burial experiments was analyzed using five factors, three levels Box-Behnken response surface design.
24299744	0	22	theme	biocomposites	24:36	arg1	behavior					12:19	Degradation behavior	0:19	Degradation behavior of biocomposites based on cassava starch	0:60	Degradation behavior of biocomposites based on cassava starch buried under indoor soil conditions.
24299744	5	23	theme	electron	683:690	arg1	microscopy					692:701	Scanning electron microscopy	674:701	Scanning electron microscopy (SEM) analysis	674:716	Scanning electron microscopy (SEM) analysis showed the formation of patterns and cracks on the surface of the materials aged in the soils.
24299744	5	23	theme	electron	683:690	arg1	SEM					704:706	SEM	704:706	SEM	704:706	Scanning electron microscopy (SEM) analysis showed the formation of patterns and cracks on the surface of the materials aged in the soils.
24299744	7	24	theme	study	915:919	arg1	results					900:906	The results	896:906	The results of the study	896:919	The results of the study demonstrated that, the tapioca starch based composites were showed a limited lifetime in biotic environment which make them suitable for being disposed in landfills after their use.
24299744	2	25	theme	starch	408:413	arg1	films					415:419	the starch films	404:419	the starch films	404:419	From the results, it was observed that, increased water sorption promotes the entry of soil microorganism and it utilizes the starch films as a source of energy for their growth.
24299744	2	25	theme	starch	408:413	arg1	source					426:431	a source	424:431	a source of energy for their growth	424:458	From the results, it was observed that, increased water sorption promotes the entry of soil microorganism and it utilizes the starch films as a source of energy for their growth.
24299744	5	26	theme	microscopy	692:701	arg1	analysis					709:716	Scanning electron microscopy (SEM) analysis	674:716	Scanning electron microscopy (SEM) analysis	674:716	Scanning electron microscopy (SEM) analysis showed the formation of patterns and cracks on the surface of the materials aged in the soils.
24299744	1	27	dep	based	139:143	arg1	starch					132:137	starch	132:137	starch	132:137	Degradation of cassava (tapioca) starch based composite films during indoor soil burial experiments was analyzed using five factors, three levels Box-Behnken response surface design.
24299744	5	28	theme	cracks	755:760	arg1	formation					729:737	the formation	725:737	the formation of patterns and cracks on the surface of the materials aged in the soils	725:810	Scanning electron microscopy (SEM) analysis showed the formation of patterns and cracks on the surface of the materials aged in the soils.
24299744	2	29	theme	energy	436:441	arg1	films					415:419	the starch films	404:419	the starch films	404:419	From the results, it was observed that, increased water sorption promotes the entry of soil microorganism and it utilizes the starch films as a source of energy for their growth.
24299744	2	29	theme	energy	436:441	arg1	source					426:431	a source	424:431	a source of energy for their growth	424:458	From the results, it was observed that, increased water sorption promotes the entry of soil microorganism and it utilizes the starch films as a source of energy for their growth.
24299744	7	30	theme	biotic	1010:1015	arg1	environment					1017:1027	biotic environment	1010:1027	biotic environment which make them suitable for being disposed in landfills after their use	1010:1100	The results of the study demonstrated that, the tapioca starch based composites were showed a limited lifetime in biotic environment which make them suitable for being disposed in landfills after their use.
24299744	5	31	theme	materials	784:792	arg1	surface					769:775	the surface	765:775	the surface of the materials aged in the soils	765:810	Scanning electron microscopy (SEM) analysis showed the formation of patterns and cracks on the surface of the materials aged in the soils.
24299744	5	32	from	soils	806:810	arg1	aged					794:797	aged	794:797	aged	794:797	Scanning electron microscopy (SEM) analysis showed the formation of patterns and cracks on the surface of the materials aged in the soils.
24299744	7	33	theme	limited	990:996	arg1	lifetime					998:1005	a limited lifetime	988:1005	a limited lifetime	988:1005	The results of the study demonstrated that, the tapioca starch based composites were showed a limited lifetime in biotic environment which make them suitable for being disposed in landfills after their use.
24299744	3	34	theme	matrix	550:555	arg1	components					557:566	matrix components	550:566	matrix components of the films	550:579	The reduction in weight and mechanical property was associated with preferential loss of matrix components of the films.
24299744	2	35	theme	increased	322:330	arg1	sorption					338:345	increased water sorption	322:345	increased water sorption	322:345	From the results, it was observed that, increased water sorption promotes the entry of soil microorganism and it utilizes the starch films as a source of energy for their growth.
24299744	0	36	theme	cassava	47:53	arg1	starch					55:60	cassava starch	47:60	cassava starch	47:60	Degradation behavior of biocomposites based on cassava starch buried under indoor soil conditions.
24299744	3	37	theme	components	557:566	arg1	loss					542:545	preferential loss	529:545	preferential loss of matrix components of the films	529:579	The reduction in weight and mechanical property was associated with preferential loss of matrix components of the films.
24299744	7	38	theme	starch	952:957	arg1	composites					965:974	the tapioca starch based composites	940:974	the tapioca starch based composites	940:974	The results of the study demonstrated that, the tapioca starch based composites were showed a limited lifetime in biotic environment which make them suitable for being disposed in landfills after their use.
24299744	7	39	theme	based	959:963	arg1	composites					965:974	the tapioca starch based composites	940:974	the tapioca starch based composites	940:974	The results of the study demonstrated that, the tapioca starch based composites were showed a limited lifetime in biotic environment which make them suitable for being disposed in landfills after their use.
24299744	5	40	theme	Scanning	674:681	arg1	microscopy					692:701	Scanning electron microscopy	674:701	Scanning electron microscopy (SEM) analysis	674:716	Scanning electron microscopy (SEM) analysis showed the formation of patterns and cracks on the surface of the materials aged in the soils.
24299744	5	40	theme	Scanning	674:681	arg1	SEM					704:706	SEM	704:706	SEM	704:706	Scanning electron microscopy (SEM) analysis showed the formation of patterns and cracks on the surface of the materials aged in the soils.
24299744	5	41	from	formation	729:737	arg1	surface					769:775	the surface	765:775	the surface of the materials aged in the soils	765:810	Scanning electron microscopy (SEM) analysis showed the formation of patterns and cracks on the surface of the materials aged in the soils.
24299744	1	42	theme	factors	223:229	arg1	design					274:279	five factors, three levels Box-Behnken response surface design	218:279	five factors, three levels Box-Behnken response surface design	218:279	Degradation of cassava (tapioca) starch based composite films during indoor soil burial experiments was analyzed using five factors, three levels Box-Behnken response surface design.
24299744	3	43	theme	films	575:579	arg1	components					557:566	matrix components	550:566	matrix components of the films	550:579	The reduction in weight and mechanical property was associated with preferential loss of matrix components of the films.
24299744	7	44	theme	tapioca	944:950	arg1	composites					965:974	the tapioca starch based composites	940:974	the tapioca starch based composites	940:974	The results of the study demonstrated that, the tapioca starch based composites were showed a limited lifetime in biotic environment which make them suitable for being disposed in landfills after their use.
24299744	1	45	theme	indoor	168:173	arg1	experiments					187:197	indoor soil burial experiments	168:197	indoor soil burial experiments	168:197	Degradation of cassava (tapioca) starch based composite films during indoor soil burial experiments was analyzed using five factors, three levels Box-Behnken response surface design.
24299744	5	46	theme	aged	794:797	arg1	materials					784:792	the materials	780:792	the materials aged in the soils	780:810	Scanning electron microscopy (SEM) analysis showed the formation of patterns and cracks on the surface of the materials aged in the soils.
24299744	1	47	theme	based	139:143	arg1	films					155:159	cassava (tapioca) starch based composite films	114:159	cassava (tapioca) starch based composite films	114:159	Degradation of cassava (tapioca) starch based composite films during indoor soil burial experiments was analyzed using five factors, three levels Box-Behnken response surface design.
25666751	5	0	theme	cytochrome	1071:1080	arg1	c					1082:1082	cytochrome c	1071:1082	cytochrome c	1071:1082	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	4	1	theme	U-2	831:833	arg1	cells					838:842	U-2 OS cells	831:842	U-2 OS cells	831:842	Our result provided the first evidence that treatment of U-2 OS cells with TRP resulted in a dose- and time-dependent inhibitory effect on cell proliferation of U-2 OS cells and caused apoptotic death.
25666751	5	2	theme	caspase-9	1136:1144	arg1	increase					981:988	an increase	978:988	an increase in Bax/Bcl-2 ratio	978:1007	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	5	2	theme	caspase-9	1136:1144	arg1	loss					1012:1015	a loss	1010:1015	a loss of mitochondrial membrane potential (Δψm)	1010:1057	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	5	2	theme	caspase-9	1136:1144	arg1	cleavage					1165:1172	cleavage	1165:1172	cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells	1165:1226	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	5	2	theme	caspase-9	1136:1144	arg1	release					1060:1066	release	1060:1066	release of cytochrome c from the mitochondria to the cytosol	1060:1119	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	5	2	theme	caspase-9	1136:1144	arg1	activation					1122:1131	activation	1122:1131	activation of caspase-9 and caspase-3	1122:1158	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	0	3	theme	mitochondrial	88:100	arg1	pathway					102:108	intrinsic mitochondrial pathway	78:108	intrinsic mitochondrial pathway in human osteosarcoma (U-2 OS) cells	78:145	A polysaccharide from Trametes robiniophila Murrill induces apoptosis through intrinsic mitochondrial pathway in human osteosarcoma (U-2 OS) cells.
25666751	4	4	from	effect	799:804	arg1	proliferation					814:826	cell proliferation	809:826	cell proliferation of U-2 OS cells	809:842	Our result provided the first evidence that treatment of U-2 OS cells with TRP resulted in a dose- and time-dependent inhibitory effect on cell proliferation of U-2 OS cells and caused apoptotic death.
25666751	4	5	theme	inhibitory	788:797	arg1	effect					799:804	time-dependent inhibitory effect	773:804	time-dependent inhibitory effect on cell proliferation of U-2 OS cells	773:842	Our result provided the first evidence that treatment of U-2 OS cells with TRP resulted in a dose- and time-dependent inhibitory effect on cell proliferation of U-2 OS cells and caused apoptotic death.
25666751	5	6	theme	Bax/Bcl-2	993:1001	arg1	ratio					1003:1007	Bax/Bcl-2 ratio	993:1007	Bax/Bcl-2 ratio	993:1007	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	1	7	theme	fruiting	234:241	arg1	bodies					243:248	the fruiting bodies	230:248	the fruiting bodies of Trametes robiniophila Murrill	230:281	In this study, we isolated and purified one homogeneous polysaccharide (TRP) from the fruiting bodies of Trametes robiniophila Murrill, and its average molecular weight was estimated to be 8.7 × 10(4) Da.
25666751	0	8	theme	human	113:117	arg1	cells					141:145	human osteosarcoma (U-2 OS) cells	113:145	human osteosarcoma (U-2 OS) cells	113:145	A polysaccharide from Trametes robiniophila Murrill induces apoptosis through intrinsic mitochondrial pathway in human osteosarcoma (U-2 OS) cells.
25666751	5	9	theme	potential	1043:1051	arg1	increase					981:988	an increase	978:988	an increase in Bax/Bcl-2 ratio	978:1007	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	5	9	theme	potential	1043:1051	arg1	loss					1012:1015	a loss	1010:1015	a loss of mitochondrial membrane potential (Δψm)	1010:1057	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	5	9	theme	potential	1043:1051	arg1	cleavage					1165:1172	cleavage	1165:1172	cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells	1165:1226	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	5	9	theme	potential	1043:1051	arg1	release					1060:1066	release	1060:1066	release of cytochrome c from the mitochondria to the cytosol	1060:1119	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	5	9	theme	potential	1043:1051	arg1	activation					1122:1131	activation	1122:1131	activation of caspase-9 and caspase-3	1122:1158	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	4	10	theme	cell	809:812	arg1	proliferation					814:826	cell proliferation	809:826	cell proliferation of U-2 OS cells	809:842	Our result provided the first evidence that treatment of U-2 OS cells with TRP resulted in a dose- and time-dependent inhibitory effect on cell proliferation of U-2 OS cells and caused apoptotic death.
25666751	5	11	theme	caspase-3	1150:1158	arg1	increase					981:988	an increase	978:988	an increase in Bax/Bcl-2 ratio	978:1007	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	5	11	theme	caspase-3	1150:1158	arg1	loss					1012:1015	a loss	1010:1015	a loss of mitochondrial membrane potential (Δψm)	1010:1057	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	5	11	theme	caspase-3	1150:1158	arg1	cleavage					1165:1172	cleavage	1165:1172	cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells	1165:1226	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	5	11	theme	caspase-3	1150:1158	arg1	release					1060:1066	release	1060:1066	release of cytochrome c from the mitochondria to the cytosol	1060:1119	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	5	11	theme	caspase-3	1150:1158	arg1	activation					1122:1131	activation	1122:1131	activation of caspase-9 and caspase-3	1122:1158	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	4	12	theme	first	694:698	arg1	evidence					700:707	the first evidence that treatment of U-2 OS cells with TRP resulted in a dose- and time-dependent inhibitory effect on cell proliferation of U-2 OS cells	690:842	the first evidence that treatment of U-2 OS cells with TRP resulted in a dose- and time-dependent inhibitory effect on cell proliferation of U-2 OS cells	690:842	Our result provided the first evidence that treatment of U-2 OS cells with TRP resulted in a dose- and time-dependent inhibitory effect on cell proliferation of U-2 OS cells and caused apoptotic death.
25666751	5	13	theme	polymerase	1194:1203	arg1	increase					981:988	an increase	978:988	an increase in Bax/Bcl-2 ratio	978:1007	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	5	13	theme	polymerase	1194:1203	arg1	loss					1012:1015	a loss	1010:1015	a loss of mitochondrial membrane potential (Δψm)	1010:1057	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	5	13	theme	polymerase	1194:1203	arg1	cleavage					1165:1172	cleavage	1165:1172	cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells	1165:1226	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	5	13	theme	polymerase	1194:1203	arg1	release					1060:1066	release	1060:1066	release of cytochrome c from the mitochondria to the cytosol	1060:1119	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	5	13	theme	polymerase	1194:1203	arg1	activation					1122:1131	activation	1122:1131	activation of caspase-9 and caspase-3	1122:1158	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	5	14	from	activation	1122:1131	arg1	ratio					1003:1007	Bax/Bcl-2 ratio	993:1007	Bax/Bcl-2 ratio	993:1007	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	5	14	from	activation	1122:1131	arg1	cells					1222:1226	U-2 OS cells	1215:1226	U-2 OS cells	1215:1226	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	7	15	theme	MTDH	1546:1549	arg1	expression					1551:1560	MTDH expression	1546:1560	MTDH expression	1546:1560	Our findings suggested that TRP inhibited the proliferation of human osteosarcoma cancer cells by promoting apoptosis through the intrinsic mitochondrial pathway, as well as inhibition of MTDH expression.
25666751	7	16	theme	cells	1447:1451	arg1	proliferation					1404:1416	the proliferation	1400:1416	the proliferation of human osteosarcoma cancer cells	1400:1451	Our findings suggested that TRP inhibited the proliferation of human osteosarcoma cancer cells by promoting apoptosis through the intrinsic mitochondrial pathway, as well as inhibition of MTDH expression.
25666751	7	17	theme	expression	1551:1560	arg1	inhibition					1532:1541	inhibition	1532:1541	inhibition of MTDH expression	1532:1560	Our findings suggested that TRP inhibited the proliferation of human osteosarcoma cancer cells by promoting apoptosis through the intrinsic mitochondrial pathway, as well as inhibition of MTDH expression.
25666751	7	17	theme	expression	1551:1560	arg1	pathway					1512:1518	the intrinsic mitochondrial pathway	1484:1518	the intrinsic mitochondrial pathway	1484:1518	Our findings suggested that TRP inhibited the proliferation of human osteosarcoma cancer cells by promoting apoptosis through the intrinsic mitochondrial pathway, as well as inhibition of MTDH expression.
25666751	4	18	theme	cells	838:842	arg1	proliferation					814:826	cell proliferation	809:826	cell proliferation of U-2 OS cells	809:842	Our result provided the first evidence that treatment of U-2 OS cells with TRP resulted in a dose- and time-dependent inhibitory effect on cell proliferation of U-2 OS cells and caused apoptotic death.
25666751	0	19	theme	osteosarcoma	119:130	arg1	cells					141:145	human osteosarcoma (U-2 OS) cells	113:145	human osteosarcoma (U-2 OS) cells	113:145	A polysaccharide from Trametes robiniophila Murrill induces apoptosis through intrinsic mitochondrial pathway in human osteosarcoma (U-2 OS) cells.
25666751	0	20	from	Murrill	44:50	arg1	polysaccharide					2:15	A polysaccharide	0:15	A polysaccharide from Trametes robiniophila Murrill	0:50	A polysaccharide from Trametes robiniophila Murrill induces apoptosis through intrinsic mitochondrial pathway in human osteosarcoma (U-2 OS) cells.
25666751	5	21	theme	mitochondrial	1020:1032	arg1	Δψm					1054:1056	Δψm	1054:1056	Δψm	1054:1056	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	5	21	theme	mitochondrial	1020:1032	arg1	potential					1043:1051	mitochondrial membrane potential	1020:1051	mitochondrial membrane potential (Δψm)	1020:1057	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	5	22	from	loss	1012:1015	arg1	ratio					1003:1007	Bax/Bcl-2 ratio	993:1007	Bax/Bcl-2 ratio	993:1007	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	5	22	from	loss	1012:1015	arg1	cells					1222:1226	U-2 OS cells	1215:1226	U-2 OS cells	1215:1226	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	5	23	from	cells	1222:1226	arg1	increase					981:988	an increase	978:988	an increase in Bax/Bcl-2 ratio	978:1007	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	5	23	from	cells	1222:1226	arg1	loss					1012:1015	a loss	1010:1015	a loss of mitochondrial membrane potential (Δψm)	1010:1057	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	5	23	from	cells	1222:1226	arg1	cleavage					1165:1172	cleavage	1165:1172	cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells	1165:1226	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	5	23	from	cells	1222:1226	arg1	release					1060:1066	release	1060:1066	release of cytochrome c from the mitochondria to the cytosol	1060:1119	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	5	23	from	cells	1222:1226	arg1	activation					1122:1131	activation	1122:1131	activation of caspase-9 and caspase-3	1122:1158	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	3	24	theme	molecular	649:657	arg1	mechanism					659:667	possible molecular mechanism	640:667	possible molecular mechanism	640:667	Particularly, we evaluated the anti-cancer efficacy of TRP on human osteosarcoma U-2 OS cells in vitro and associated possible molecular mechanism.
25666751	4	25	theme	time-dependent	773:786	arg1	effect					799:804	time-dependent inhibitory effect	773:804	time-dependent inhibitory effect on cell proliferation of U-2 OS cells	773:842	Our result provided the first evidence that treatment of U-2 OS cells with TRP resulted in a dose- and time-dependent inhibitory effect on cell proliferation of U-2 OS cells and caused apoptotic death.
25666751	4	26	with	treatment	714:722	arg1	TRP					745:747	TRP	745:747	TRP	745:747	Our result provided the first evidence that treatment of U-2 OS cells with TRP resulted in a dose- and time-dependent inhibitory effect on cell proliferation of U-2 OS cells and caused apoptotic death.
25666751	5	27	from	cleavage	1165:1172	arg1	ratio					1003:1007	Bax/Bcl-2 ratio	993:1007	Bax/Bcl-2 ratio	993:1007	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	5	27	from	cleavage	1165:1172	arg1	cells					1222:1226	U-2 OS cells	1215:1226	U-2 OS cells	1215:1226	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	3	28	from	efficacy	565:572	arg1	cells					610:614	human osteosarcoma U-2 OS cells	584:614	human osteosarcoma U-2 OS cells	584:614	Particularly, we evaluated the anti-cancer efficacy of TRP on human osteosarcoma U-2 OS cells in vitro and associated possible molecular mechanism.
25666751	5	29	theme	membrane	1034:1041	arg1	Δψm					1054:1056	Δψm	1054:1056	Δψm	1054:1056	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	5	29	theme	membrane	1034:1041	arg1	potential					1043:1051	mitochondrial membrane potential	1020:1051	mitochondrial membrane potential (Δψm)	1020:1057	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	1	30	theme	Trametes	253:260	arg1	Murrill					275:281	Trametes robiniophila Murrill	253:281	Trametes robiniophila Murrill	253:281	In this study, we isolated and purified one homogeneous polysaccharide (TRP) from the fruiting bodies of Trametes robiniophila Murrill, and its average molecular weight was estimated to be 8.7 × 10(4) Da.
25666751	3	31	theme	U-2	603:605	arg1	cells					610:614	human osteosarcoma U-2 OS cells	584:614	human osteosarcoma U-2 OS cells	584:614	Particularly, we evaluated the anti-cancer efficacy of TRP on human osteosarcoma U-2 OS cells in vitro and associated possible molecular mechanism.
25666751	7	32	theme	human	1421:1425	arg1	cells					1447:1451	human osteosarcoma cancer cells	1421:1451	human osteosarcoma cancer cells	1421:1451	Our findings suggested that TRP inhibited the proliferation of human osteosarcoma cancer cells by promoting apoptosis through the intrinsic mitochondrial pathway, as well as inhibition of MTDH expression.
25666751	5	33	theme	poly	1177:1180	arg1	PARP					1206:1209	PARP	1206:1209	PARP	1206:1209	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	5	33	theme	poly	1177:1180	arg1	polymerase					1194:1203	poly(ADP-ribose) polymerase	1177:1203	poly(ADP-ribose) polymerase (PARP) in U-2 OS cells	1177:1226	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	3	34	theme	human	584:588	arg1	cells					610:614	human osteosarcoma U-2 OS cells	584:614	human osteosarcoma U-2 OS cells	584:614	Particularly, we evaluated the anti-cancer efficacy of TRP on human osteosarcoma U-2 OS cells in vitro and associated possible molecular mechanism.
25666751	2	35	theme	gas	392:394	arg1	chromatography					396:409	gas chromatography	392:409	gas chromatography (GC)	392:414	Monosaccharide composition analysis by gas chromatography (GC) indicated that TRP was composed of glucose, galactose, and arabinose in the molar ratio of 4.2:1.10:1.06.
25666751	2	35	theme	gas	392:394	arg1	GC					412:413	GC	412:413	GC	412:413	Monosaccharide composition analysis by gas chromatography (GC) indicated that TRP was composed of glucose, galactose, and arabinose in the molar ratio of 4.2:1.10:1.06.
25666751	1	36	theme	robiniophila	262:273	arg1	Murrill					275:281	Trametes robiniophila Murrill	253:281	Trametes robiniophila Murrill	253:281	In this study, we isolated and purified one homogeneous polysaccharide (TRP) from the fruiting bodies of Trametes robiniophila Murrill, and its average molecular weight was estimated to be 8.7 × 10(4) Da.
25666751	0	37	theme	OS	137:138	arg1	cells					141:145	human osteosarcoma (U-2 OS) cells	113:145	human osteosarcoma (U-2 OS) cells	113:145	A polysaccharide from Trametes robiniophila Murrill induces apoptosis through intrinsic mitochondrial pathway in human osteosarcoma (U-2 OS) cells.
25666751	7	38	theme	mitochondrial	1498:1510	arg1	pathway					1512:1518	the intrinsic mitochondrial pathway	1484:1518	the intrinsic mitochondrial pathway	1484:1518	Our findings suggested that TRP inhibited the proliferation of human osteosarcoma cancer cells by promoting apoptosis through the intrinsic mitochondrial pathway, as well as inhibition of MTDH expression.
25666751	1	39	theme	Murrill	275:281	arg1	bodies					243:248	the fruiting bodies	230:248	the fruiting bodies of Trametes robiniophila Murrill	230:281	In this study, we isolated and purified one homogeneous polysaccharide (TRP) from the fruiting bodies of Trametes robiniophila Murrill, and its average molecular weight was estimated to be 8.7 × 10(4) Da.
25666751	0	40	theme	U-2	133:135	arg1	cells					141:145	human osteosarcoma (U-2 OS) cells	113:145	human osteosarcoma (U-2 OS) cells	113:145	A polysaccharide from Trametes robiniophila Murrill induces apoptosis through intrinsic mitochondrial pathway in human osteosarcoma (U-2 OS) cells.
25666751	0	41	from	pathway	102:108	arg1	cells					141:145	human osteosarcoma (U-2 OS) cells	113:145	human osteosarcoma (U-2 OS) cells	113:145	A polysaccharide from Trametes robiniophila Murrill induces apoptosis through intrinsic mitochondrial pathway in human osteosarcoma (U-2 OS) cells.
25666751	4	42	from	dose-	763:767	arg1	proliferation					814:826	cell proliferation	809:826	cell proliferation of U-2 OS cells	809:842	Our result provided the first evidence that treatment of U-2 OS cells with TRP resulted in a dose- and time-dependent inhibitory effect on cell proliferation of U-2 OS cells and caused apoptotic death.
25666751	5	43	theme	ADP-ribose	1182:1191	arg1	PARP					1206:1209	PARP	1206:1209	PARP	1206:1209	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	5	43	theme	ADP-ribose	1182:1191	arg1	polymerase					1194:1203	poly(ADP-ribose) polymerase	1177:1203	poly(ADP-ribose) polymerase (PARP) in U-2 OS cells	1177:1226	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	2	44	theme	composition	368:378	arg1	analysis					380:387	Monosaccharide composition analysis	353:387	Monosaccharide composition analysis by gas chromatography (GC)	353:414	Monosaccharide composition analysis by gas chromatography (GC) indicated that TRP was composed of glucose, galactose, and arabinose in the molar ratio of 4.2:1.10:1.06.
25666751	5	45	theme	c	1082:1082	arg1	increase					981:988	an increase	978:988	an increase in Bax/Bcl-2 ratio	978:1007	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	5	45	theme	c	1082:1082	arg1	loss					1012:1015	a loss	1010:1015	a loss of mitochondrial membrane potential (Δψm)	1010:1057	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	5	45	theme	c	1082:1082	arg1	cleavage					1165:1172	cleavage	1165:1172	cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells	1165:1226	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	5	45	theme	c	1082:1082	arg1	release					1060:1066	release	1060:1066	release of cytochrome c from the mitochondria to the cytosol	1060:1119	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	5	45	theme	c	1082:1082	arg1	activation					1122:1131	activation	1122:1131	activation of caspase-9 and caspase-3	1122:1158	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	5	46	theme	cells	918:922	arg1	apoptosis					898:906	the apoptosis	894:906	the apoptosis of U-2 OS cells	894:922	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	4	47	theme	apoptotic	855:863	arg1	death					865:869	apoptotic death	855:869	apoptotic death	855:869	Our result provided the first evidence that treatment of U-2 OS cells with TRP resulted in a dose- and time-dependent inhibitory effect on cell proliferation of U-2 OS cells and caused apoptotic death.
25666751	2	48	theme	Monosaccharide	353:366	arg1	analysis					380:387	Monosaccharide composition analysis	353:387	Monosaccharide composition analysis by gas chromatography (GC)	353:414	Monosaccharide composition analysis by gas chromatography (GC) indicated that TRP was composed of glucose, galactose, and arabinose in the molar ratio of 4.2:1.10:1.06.
25666751	5	49	from	polymerase	1194:1203	arg1	cells					1222:1226	U-2 OS cells	1215:1226	U-2 OS cells	1215:1226	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	1	50	theme	homogeneous	192:202	arg1	TRP					220:222	TRP	220:222	TRP	220:222	In this study, we isolated and purified one homogeneous polysaccharide (TRP) from the fruiting bodies of Trametes robiniophila Murrill, and its average molecular weight was estimated to be 8.7 × 10(4) Da.
25666751	1	50	theme	homogeneous	192:202	arg1	polysaccharide					204:217	one homogeneous polysaccharide	188:217	one homogeneous polysaccharide (TRP)	188:223	In this study, we isolated and purified one homogeneous polysaccharide (TRP) from the fruiting bodies of Trametes robiniophila Murrill, and its average molecular weight was estimated to be 8.7 × 10(4) Da.
25666751	0	51	theme	robiniophila	31:42	arg1	Murrill					44:50	Trametes robiniophila Murrill	22:50	Trametes robiniophila Murrill	22:50	A polysaccharide from Trametes robiniophila Murrill induces apoptosis through intrinsic mitochondrial pathway in human osteosarcoma (U-2 OS) cells.
25666751	7	52	theme	osteosarcoma	1427:1438	arg1	cells					1447:1451	human osteosarcoma cancer cells	1421:1451	human osteosarcoma cancer cells	1421:1451	Our findings suggested that TRP inhibited the proliferation of human osteosarcoma cancer cells by promoting apoptosis through the intrinsic mitochondrial pathway, as well as inhibition of MTDH expression.
25666751	6	53	theme	metadherin	1260:1269	arg1	overexpression					1242:1255	overexpression	1242:1255	overexpression of metadherin (MTDH), one carcinogene,	1242:1294	In addition, overexpression of metadherin (MTDH), one carcinogene, was inhibited in U-2 OS cells after exposure to TRP for 24 h.
25666751	0	54	theme	Trametes	22:29	arg1	Murrill					44:50	Trametes robiniophila Murrill	22:50	Trametes robiniophila Murrill	22:50	A polysaccharide from Trametes robiniophila Murrill induces apoptosis through intrinsic mitochondrial pathway in human osteosarcoma (U-2 OS) cells.
25666751	5	55	from	increase	981:988	arg1	ratio					1003:1007	Bax/Bcl-2 ratio	993:1007	Bax/Bcl-2 ratio	993:1007	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	5	55	from	increase	981:988	arg1	cells					1222:1226	U-2 OS cells	1215:1226	U-2 OS cells	1215:1226	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	7	56	theme	cancer	1440:1445	arg1	cells					1447:1451	human osteosarcoma cancer cells	1421:1451	human osteosarcoma cancer cells	1421:1451	Our findings suggested that TRP inhibited the proliferation of human osteosarcoma cancer cells by promoting apoptosis through the intrinsic mitochondrial pathway, as well as inhibition of MTDH expression.
25666751	2	57	theme	4.2:1.10:1.06	507:519	arg1	ratio					498:502	the molar ratio	488:502	the molar ratio of 4.2:1.10:1.06	488:519	Monosaccharide composition analysis by gas chromatography (GC) indicated that TRP was composed of glucose, galactose, and arabinose in the molar ratio of 4.2:1.10:1.06.
25666751	3	58	theme	TRP	577:579	arg1	efficacy					565:572	the anti-cancer efficacy	549:572	the anti-cancer efficacy of TRP on human osteosarcoma U-2 OS cells	549:614	Particularly, we evaluated the anti-cancer efficacy of TRP on human osteosarcoma U-2 OS cells in vitro and associated possible molecular mechanism.
25666751	5	59	theme	mitochondria-dependent	930:951	arg1	pathway					953:959	a mitochondria-dependent pathway	928:959	a mitochondria-dependent pathway	928:959	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	1	60	theme	×	341:341	arg1	Da					349:350	8.7 × 10(4) Da	337:350	8.7 × 10(4) Da	337:350	In this study, we isolated and purified one homogeneous polysaccharide (TRP) from the fruiting bodies of Trametes robiniophila Murrill, and its average molecular weight was estimated to be 8.7 × 10(4) Da.
25666751	1	61	theme	average	292:298	arg1	weight					310:315	its average molecular weight	288:315	its average molecular weight	288:315	In this study, we isolated and purified one homogeneous polysaccharide (TRP) from the fruiting bodies of Trametes robiniophila Murrill, and its average molecular weight was estimated to be 8.7 × 10(4) Da.
25666751	5	62	from	mitochondria	1093:1104	arg1	increase					981:988	an increase	978:988	an increase in Bax/Bcl-2 ratio	978:1007	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	5	62	from	mitochondria	1093:1104	arg1	loss					1012:1015	a loss	1010:1015	a loss of mitochondrial membrane potential (Δψm)	1010:1057	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	5	62	from	mitochondria	1093:1104	arg1	cleavage					1165:1172	cleavage	1165:1172	cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells	1165:1226	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	5	62	from	mitochondria	1093:1104	arg1	release					1060:1066	release	1060:1066	release of cytochrome c from the mitochondria to the cytosol	1060:1119	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	5	62	from	mitochondria	1093:1104	arg1	activation					1122:1131	activation	1122:1131	activation of caspase-9 and caspase-3	1122:1158	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	3	63	theme	OS	607:608	arg1	cells					610:614	human osteosarcoma U-2 OS cells	584:614	human osteosarcoma U-2 OS cells	584:614	Particularly, we evaluated the anti-cancer efficacy of TRP on human osteosarcoma U-2 OS cells in vitro and associated possible molecular mechanism.
25666751	5	64	theme	OS	915:916	arg1	cells					918:922	U-2 OS cells	911:922	U-2 OS cells	911:922	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	1	65	theme	molecular	300:308	arg1	weight					310:315	its average molecular weight	288:315	its average molecular weight	288:315	In this study, we isolated and purified one homogeneous polysaccharide (TRP) from the fruiting bodies of Trametes robiniophila Murrill, and its average molecular weight was estimated to be 8.7 × 10(4) Da.
25666751	4	66	theme	OS	835:836	arg1	cells					838:842	U-2 OS cells	831:842	U-2 OS cells	831:842	Our result provided the first evidence that treatment of U-2 OS cells with TRP resulted in a dose- and time-dependent inhibitory effect on cell proliferation of U-2 OS cells and caused apoptotic death.
25666751	2	67	from	glucose	451:457	arg1	ratio					498:502	the molar ratio	488:502	the molar ratio of 4.2:1.10:1.06	488:519	Monosaccharide composition analysis by gas chromatography (GC) indicated that TRP was composed of glucose, galactose, and arabinose in the molar ratio of 4.2:1.10:1.06.
25666751	3	68	theme	anti-cancer	553:563	arg1	efficacy					565:572	the anti-cancer efficacy	549:572	the anti-cancer efficacy of TRP on human osteosarcoma U-2 OS cells	549:614	Particularly, we evaluated the anti-cancer efficacy of TRP on human osteosarcoma U-2 OS cells in vitro and associated possible molecular mechanism.
25666751	5	69	from	release	1060:1066	arg1	ratio					1003:1007	Bax/Bcl-2 ratio	993:1007	Bax/Bcl-2 ratio	993:1007	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	5	69	from	release	1060:1066	arg1	cells					1222:1226	U-2 OS cells	1215:1226	U-2 OS cells	1215:1226	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	7	70	theme	intrinsic	1488:1496	arg1	pathway					1512:1518	the intrinsic mitochondrial pathway	1484:1518	the intrinsic mitochondrial pathway	1484:1518	Our findings suggested that TRP inhibited the proliferation of human osteosarcoma cancer cells by promoting apoptosis through the intrinsic mitochondrial pathway, as well as inhibition of MTDH expression.
25666751	6	71	theme	OS	1317:1318	arg1	cells					1320:1324	U-2 OS cells	1313:1324	U-2 OS cells	1313:1324	In addition, overexpression of metadherin (MTDH), one carcinogene, was inhibited in U-2 OS cells after exposure to TRP for 24 h.
25666751	2	72	from	arabinose	475:483	arg1	ratio					498:502	the molar ratio	488:502	the molar ratio of 4.2:1.10:1.06	488:519	Monosaccharide composition analysis by gas chromatography (GC) indicated that TRP was composed of glucose, galactose, and arabinose in the molar ratio of 4.2:1.10:1.06.
25666751	2	73	theme	molar	492:496	arg1	ratio					498:502	the molar ratio	488:502	the molar ratio of 4.2:1.10:1.06	488:519	Monosaccharide composition analysis by gas chromatography (GC) indicated that TRP was composed of glucose, galactose, and arabinose in the molar ratio of 4.2:1.10:1.06.
25666751	3	74	theme	osteosarcoma	590:601	arg1	cells					610:614	human osteosarcoma U-2 OS cells	584:614	human osteosarcoma U-2 OS cells	584:614	Particularly, we evaluated the anti-cancer efficacy of TRP on human osteosarcoma U-2 OS cells in vitro and associated possible molecular mechanism.
25666751	5	75	theme	U-2	911:913	arg1	cells					918:922	U-2 OS cells	911:922	U-2 OS cells	911:922	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	6	76	theme	U-2	1313:1315	arg1	cells					1320:1324	U-2 OS cells	1313:1324	U-2 OS cells	1313:1324	In addition, overexpression of metadherin (MTDH), one carcinogene, was inhibited in U-2 OS cells after exposure to TRP for 24 h.
25666751	4	77	theme	U-2	727:729	arg1	cells					734:738	U-2 OS cells	727:738	U-2 OS cells	727:738	Our result provided the first evidence that treatment of U-2 OS cells with TRP resulted in a dose- and time-dependent inhibitory effect on cell proliferation of U-2 OS cells and caused apoptotic death.
25666751	0	78	theme	intrinsic	78:86	arg1	pathway					102:108	intrinsic mitochondrial pathway	78:108	intrinsic mitochondrial pathway in human osteosarcoma (U-2 OS) cells	78:145	A polysaccharide from Trametes robiniophila Murrill induces apoptosis through intrinsic mitochondrial pathway in human osteosarcoma (U-2 OS) cells.
25666751	2	79	from	galactose	460:468	arg1	ratio					498:502	the molar ratio	488:502	the molar ratio of 4.2:1.10:1.06	488:519	Monosaccharide composition analysis by gas chromatography (GC) indicated that TRP was composed of glucose, galactose, and arabinose in the molar ratio of 4.2:1.10:1.06.
25666751	4	80	theme	cells	734:738	arg1	treatment					714:722	treatment	714:722	treatment of U-2 OS cells with TRP	714:747	Our result provided the first evidence that treatment of U-2 OS cells with TRP resulted in a dose- and time-dependent inhibitory effect on cell proliferation of U-2 OS cells and caused apoptotic death.
25666751	5	81	theme	U-2	1215:1217	arg1	cells					1222:1226	U-2 OS cells	1215:1226	U-2 OS cells	1215:1226	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	4	82	theme	OS	731:732	arg1	cells					734:738	U-2 OS cells	727:738	U-2 OS cells	727:738	Our result provided the first evidence that treatment of U-2 OS cells with TRP resulted in a dose- and time-dependent inhibitory effect on cell proliferation of U-2 OS cells and caused apoptotic death.
25666751	5	83	theme	OS	1219:1220	arg1	cells					1222:1226	U-2 OS cells	1215:1226	U-2 OS cells	1215:1226	Moreover, TRP induced the apoptosis of U-2 OS cells via a mitochondria-dependent pathway, as evidenced by an increase in Bax/Bcl-2 ratio, a loss of mitochondrial membrane potential (Δψm), release of cytochrome c from the mitochondria to the cytosol, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells.
25666751	3	84	theme	possible	640:647	arg1	mechanism					659:667	possible molecular mechanism	640:667	possible molecular mechanism	640:667	Particularly, we evaluated the anti-cancer efficacy of TRP on human osteosarcoma U-2 OS cells in vitro and associated possible molecular mechanism.
26277748	4	0	theme	carbohydrate	960:971	arg1	region					973:978	the carbohydrate region	956:978	the carbohydrate region	956:978	AAPF and AAPP were characterized by Fourier Transform Infrared spectroscopy (FTIR), which indicated peaks at approximately 3500cm(-1) and 522.28cm(-1) in the carbohydrate region and composed of β-glycoside linkages at the 1200-1000cm(-1) peaks.
26277748	4	1	theme	1200-1000cm	1024:1034	arg1	peaks					1040:1044	the 1200-1000cm(-1) peaks	1020:1044	the 1200-1000cm(-1) peaks	1020:1044	AAPF and AAPP were characterized by Fourier Transform Infrared spectroscopy (FTIR), which indicated peaks at approximately 3500cm(-1) and 522.28cm(-1) in the carbohydrate region and composed of β-glycoside linkages at the 1200-1000cm(-1) peaks.
26277748	1	2	theme	pickled	271:277	arg1	product					279:285	pickled product	271:285	pickled product	271:285	The study planned to determine proximate composition, antioxidant activity and chemical characterization in the fruit bodies of Auricularia auricula and pickled product.
26277748	0	3	theme	fresh	91:95	arg1	product					109:115	fresh and pickled product	91:115	fresh and pickled product	91:115	Characterization of Auricularia auricula polysaccharides and its antioxidant properties in fresh and pickled product.
26277748	1	4	theme	antioxidant	172:182	arg1	activity					184:191	antioxidant activity	172:191	antioxidant activity	172:191	The study planned to determine proximate composition, antioxidant activity and chemical characterization in the fruit bodies of Auricularia auricula and pickled product.
26277748	1	5	theme	product	279:285	arg1	bodies					236:241	the fruit bodies	226:241	the fruit bodies of Auricularia auricula and pickled product	226:285	The study planned to determine proximate composition, antioxidant activity and chemical characterization in the fruit bodies of Auricularia auricula and pickled product.
26277748	3	6	with	galactose	597:605	arg1	ratio					620:624	a molar ratio	612:624	a molar ratio of 16.74:1.0:1.18:1.0:1.0	612:650	The results showed that AAPF consisted of five monosaccharides, including glucose, rhamnose, arabinose, mannose and galactose with a molar ratio of 16.74:1.0:1.18:1.0:1.0; in contrast, AAPP was composed of four monosaccharides, namely arabinose, mannose, galactose and xylose with the molar ratio of 15.59:1.52:4.76:1.0.
26277748	0	7	from	Characterization	0:15	arg1	product					109:115	fresh and pickled product	91:115	fresh and pickled product	91:115	Characterization of Auricularia auricula polysaccharides and its antioxidant properties in fresh and pickled product.
26277748	5	8	from	result	1116:1121	arg1	radicals					1142:1149	scavenging free radicals	1126:1149	scavenging free radicals	1126:1149	However, the antioxidant activity of AAPF demonstrated a significant result in scavenging free radicals except for DDPH.
26277748	4	9	from	522.28cm	940:947	arg1	peaks					902:906	peaks	902:906	peaks at approximately 3500cm(-1) and 522.28cm(-1) in the carbohydrate region	902:978	AAPF and AAPP were characterized by Fourier Transform Infrared spectroscopy (FTIR), which indicated peaks at approximately 3500cm(-1) and 522.28cm(-1) in the carbohydrate region and composed of β-glycoside linkages at the 1200-1000cm(-1) peaks.
26277748	0	10	theme	pickled	101:107	arg1	product					109:115	fresh and pickled product	91:115	fresh and pickled product	91:115	Characterization of Auricularia auricula polysaccharides and its antioxidant properties in fresh and pickled product.
26277748	6	11	theme	antioxidant	1225:1235	arg1	activity					1237:1244	good antioxidant activity	1220:1244	good antioxidant activity	1220:1244	The in vitro test indicated that AAPF and AAPP have good antioxidant activity, demonstrating that A. auricula polysaccharide has immense potential as a functional food.
26277748	6	12	dep	in	1172:1173	arg1	vitro					1175:1179	vitro	1175:1179	vitro	1175:1179	The in vitro test indicated that AAPF and AAPP have good antioxidant activity, demonstrating that A. auricula polysaccharide has immense potential as a functional food.
26277748	6	13	theme	functional	1320:1329	arg1	food					1331:1334	a functional food	1318:1334	a functional food	1318:1334	The in vitro test indicated that AAPF and AAPP have good antioxidant activity, demonstrating that A. auricula polysaccharide has immense potential as a functional food.
26277748	6	13	theme	functional	1320:1329	arg1	potential					1305:1313	immense potential	1297:1313	immense potential	1297:1313	The in vitro test indicated that AAPF and AAPP have good antioxidant activity, demonstrating that A. auricula polysaccharide has immense potential as a functional food.
26277748	5	14	theme	antioxidant	1060:1070	arg1	activity					1072:1079	the antioxidant activity	1056:1079	the antioxidant activity of AAPF	1056:1087	However, the antioxidant activity of AAPF demonstrated a significant result in scavenging free radicals except for DDPH.
26277748	4	15	theme	Infrared	856:863	arg1	spectroscopy					865:876	Infrared spectroscopy	856:876	Infrared spectroscopy (FTIR)	856:883	AAPF and AAPP were characterized by Fourier Transform Infrared spectroscopy (FTIR), which indicated peaks at approximately 3500cm(-1) and 522.28cm(-1) in the carbohydrate region and composed of β-glycoside linkages at the 1200-1000cm(-1) peaks.
26277748	4	15	theme	Infrared	856:863	arg1	FTIR					879:882	FTIR	879:882	FTIR	879:882	AAPF and AAPP were characterized by Fourier Transform Infrared spectroscopy (FTIR), which indicated peaks at approximately 3500cm(-1) and 522.28cm(-1) in the carbohydrate region and composed of β-glycoside linkages at the 1200-1000cm(-1) peaks.
26277748	1	16	from	composition	159:169	arg1	bodies					236:241	the fruit bodies	226:241	the fruit bodies of Auricularia auricula and pickled product	226:285	The study planned to determine proximate composition, antioxidant activity and chemical characterization in the fruit bodies of Auricularia auricula and pickled product.
26277748	1	17	theme	chemical	197:204	arg1	characterization					206:221	chemical characterization	197:221	chemical characterization	197:221	The study planned to determine proximate composition, antioxidant activity and chemical characterization in the fruit bodies of Auricularia auricula and pickled product.
26277748	2	18	theme	polysaccharide	292:305	arg1	AAPP					324:327	AAPP	324:327	AAPP	324:327	Two polysaccharide fractions (AAPF, AAPP) were purified from the fruiting body of A. auricula by ethanol precipitation, deproteination and macroporous resins and were subsequently lyophilized.
26277748	2	18	theme	polysaccharide	292:305	arg1	fractions					307:315	Two polysaccharide fractions	288:315	Two polysaccharide fractions (AAPF, AAPP)	288:328	Two polysaccharide fractions (AAPF, AAPP) were purified from the fruiting body of A. auricula by ethanol precipitation, deproteination and macroporous resins and were subsequently lyophilized.
26277748	5	19	theme	scavenging	1126:1135	arg1	radicals					1142:1149	scavenging free radicals	1126:1149	scavenging free radicals	1126:1149	However, the antioxidant activity of AAPF demonstrated a significant result in scavenging free radicals except for DDPH.
26277748	3	20	with	arabinose	574:582	arg1	ratio					620:624	a molar ratio	612:624	a molar ratio of 16.74:1.0:1.18:1.0:1.0	612:650	The results showed that AAPF consisted of five monosaccharides, including glucose, rhamnose, arabinose, mannose and galactose with a molar ratio of 16.74:1.0:1.18:1.0:1.0; in contrast, AAPP was composed of four monosaccharides, namely arabinose, mannose, galactose and xylose with the molar ratio of 15.59:1.52:4.76:1.0.
26277748	3	21	with	xylose	750:755	arg1	ratio					772:776	the molar ratio	762:776	the molar ratio of 15.59:1.52:4.76:1.0	762:799	The results showed that AAPF consisted of five monosaccharides, including glucose, rhamnose, arabinose, mannose and galactose with a molar ratio of 16.74:1.0:1.18:1.0:1.0; in contrast, AAPP was composed of four monosaccharides, namely arabinose, mannose, galactose and xylose with the molar ratio of 15.59:1.52:4.76:1.0.
26277748	2	22	theme	ethanol	385:391	arg1	precipitation					393:405	ethanol precipitation	385:405	ethanol precipitation	385:405	Two polysaccharide fractions (AAPF, AAPP) were purified from the fruiting body of A. auricula by ethanol precipitation, deproteination and macroporous resins and were subsequently lyophilized.
26277748	4	23	theme	β-glycoside	996:1006	arg1	linkages					1008:1015	β-glycoside linkages	996:1015	β-glycoside linkages at the 1200-1000cm(-1) peaks	996:1044	AAPF and AAPP were characterized by Fourier Transform Infrared spectroscopy (FTIR), which indicated peaks at approximately 3500cm(-1) and 522.28cm(-1) in the carbohydrate region and composed of β-glycoside linkages at the 1200-1000cm(-1) peaks.
26277748	3	24	with	rhamnose	564:571	arg1	ratio					620:624	a molar ratio	612:624	a molar ratio of 16.74:1.0:1.18:1.0:1.0	612:650	The results showed that AAPF consisted of five monosaccharides, including glucose, rhamnose, arabinose, mannose and galactose with a molar ratio of 16.74:1.0:1.18:1.0:1.0; in contrast, AAPP was composed of four monosaccharides, namely arabinose, mannose, galactose and xylose with the molar ratio of 15.59:1.52:4.76:1.0.
26277748	6	25	theme	good	1220:1223	arg1	activity					1237:1244	good antioxidant activity	1220:1244	good antioxidant activity	1220:1244	The in vitro test indicated that AAPF and AAPP have good antioxidant activity, demonstrating that A. auricula polysaccharide has immense potential as a functional food.
26277748	0	26	theme	auricula	32:39	arg1	polysaccharides					41:55	Auricularia auricula polysaccharides	20:55	Auricularia auricula polysaccharides	20:55	Characterization of Auricularia auricula polysaccharides and its antioxidant properties in fresh and pickled product.
26277748	4	27	dep	Fourier	838:844	arg1	Transform					846:854	Transform	846:854	Transform Infrared spectroscopy (FTIR)	846:883	AAPF and AAPP were characterized by Fourier Transform Infrared spectroscopy (FTIR), which indicated peaks at approximately 3500cm(-1) and 522.28cm(-1) in the carbohydrate region and composed of β-glycoside linkages at the 1200-1000cm(-1) peaks.
26277748	0	28	theme	Auricularia	20:30	arg1	polysaccharides					41:55	Auricularia auricula polysaccharides	20:55	Auricularia auricula polysaccharides	20:55	Characterization of Auricularia auricula polysaccharides and its antioxidant properties in fresh and pickled product.
26277748	2	29	theme	auricula	373:380	arg1	body					362:365	the fruiting body	349:365	the fruiting body of A. auricula	349:380	Two polysaccharide fractions (AAPF, AAPP) were purified from the fruiting body of A. auricula by ethanol precipitation, deproteination and macroporous resins and were subsequently lyophilized.
26277748	6	30	contain	have	1215:1218	arg2	activity					1237:1244	good antioxidant activity	1220:1244	good antioxidant activity	1220:1244	The in vitro test indicated that AAPF and AAPP have good antioxidant activity, demonstrating that A. auricula polysaccharide has immense potential as a functional food.
26277748	6	30	contain	have	1215:1218	arg1	AAPP					1210:1213	AAPP	1210:1213	AAPP	1210:1213	The in vitro test indicated that AAPF and AAPP have good antioxidant activity, demonstrating that A. auricula polysaccharide has immense potential as a functional food.
26277748	6	30	contain	have	1215:1218	arg1	AAPF					1201:1204	AAPF	1201:1204	AAPF	1201:1204	The in vitro test indicated that AAPF and AAPP have good antioxidant activity, demonstrating that A. auricula polysaccharide has immense potential as a functional food.
26277748	2	31	theme	macroporous	427:437	arg1	resins					439:444	macroporous resins	427:444	macroporous resins	427:444	Two polysaccharide fractions (AAPF, AAPP) were purified from the fruiting body of A. auricula by ethanol precipitation, deproteination and macroporous resins and were subsequently lyophilized.
26277748	3	32	with	glucose	555:561	arg1	ratio					620:624	a molar ratio	612:624	a molar ratio of 16.74:1.0:1.18:1.0:1.0	612:650	The results showed that AAPF consisted of five monosaccharides, including glucose, rhamnose, arabinose, mannose and galactose with a molar ratio of 16.74:1.0:1.18:1.0:1.0; in contrast, AAPP was composed of four monosaccharides, namely arabinose, mannose, galactose and xylose with the molar ratio of 15.59:1.52:4.76:1.0.
26277748	6	33	theme	immense	1297:1303	arg1	food					1331:1334	a functional food	1318:1334	a functional food	1318:1334	The in vitro test indicated that AAPF and AAPP have good antioxidant activity, demonstrating that A. auricula polysaccharide has immense potential as a functional food.
26277748	6	33	theme	immense	1297:1303	arg1	potential					1305:1313	immense potential	1297:1313	immense potential	1297:1313	The in vitro test indicated that AAPF and AAPP have good antioxidant activity, demonstrating that A. auricula polysaccharide has immense potential as a functional food.
26277748	1	34	theme	fruit	230:234	arg1	bodies					236:241	the fruit bodies	226:241	the fruit bodies of Auricularia auricula and pickled product	226:285	The study planned to determine proximate composition, antioxidant activity and chemical characterization in the fruit bodies of Auricularia auricula and pickled product.
26277748	0	35	theme	polysaccharides	41:55	arg1	Characterization					0:15	Characterization	0:15	Characterization of Auricularia auricula polysaccharides and its antioxidant properties in fresh and pickled product.	0:116	Characterization of Auricularia auricula polysaccharides and its antioxidant properties in fresh and pickled product.
26277748	3	36	theme	molar	614:618	arg1	ratio					620:624	a molar ratio	612:624	a molar ratio of 16.74:1.0:1.18:1.0:1.0	612:650	The results showed that AAPF consisted of five monosaccharides, including glucose, rhamnose, arabinose, mannose and galactose with a molar ratio of 16.74:1.0:1.18:1.0:1.0; in contrast, AAPP was composed of four monosaccharides, namely arabinose, mannose, galactose and xylose with the molar ratio of 15.59:1.52:4.76:1.0.
26277748	5	37	theme	free	1137:1140	arg1	radicals					1142:1149	scavenging free radicals	1126:1149	scavenging free radicals	1126:1149	However, the antioxidant activity of AAPF demonstrated a significant result in scavenging free radicals except for DDPH.
26277748	0	38	theme	antioxidant	65:75	arg1	properties					77:86	its antioxidant properties	61:86	its antioxidant properties	61:86	Characterization of Auricularia auricula polysaccharides and its antioxidant properties in fresh and pickled product.
26277748	3	39	theme	15.59:1.52:4.76:1.0	781:799	arg1	ratio					772:776	the molar ratio	762:776	the molar ratio of 15.59:1.52:4.76:1.0	762:799	The results showed that AAPF consisted of five monosaccharides, including glucose, rhamnose, arabinose, mannose and galactose with a molar ratio of 16.74:1.0:1.18:1.0:1.0; in contrast, AAPP was composed of four monosaccharides, namely arabinose, mannose, galactose and xylose with the molar ratio of 15.59:1.52:4.76:1.0.
26277748	4	40	from	3500cm	925:930	arg1	peaks					902:906	peaks	902:906	peaks at approximately 3500cm(-1) and 522.28cm(-1) in the carbohydrate region	902:978	AAPF and AAPP were characterized by Fourier Transform Infrared spectroscopy (FTIR), which indicated peaks at approximately 3500cm(-1) and 522.28cm(-1) in the carbohydrate region and composed of β-glycoside linkages at the 1200-1000cm(-1) peaks.
26277748	2	41	theme	fruiting	353:360	arg1	body					362:365	the fruiting body	349:365	the fruiting body of A. auricula	349:380	Two polysaccharide fractions (AAPF, AAPP) were purified from the fruiting body of A. auricula by ethanol precipitation, deproteination and macroporous resins and were subsequently lyophilized.
26277748	5	42	theme	significant	1104:1114	arg1	result					1116:1121	a significant result	1102:1121	a significant result in scavenging free radicals	1102:1149	However, the antioxidant activity of AAPF demonstrated a significant result in scavenging free radicals except for DDPH.
26277748	3	43	theme	molar	766:770	arg1	ratio					772:776	the molar ratio	762:776	the molar ratio of 15.59:1.52:4.76:1.0	762:799	The results showed that AAPF consisted of five monosaccharides, including glucose, rhamnose, arabinose, mannose and galactose with a molar ratio of 16.74:1.0:1.18:1.0:1.0; in contrast, AAPP was composed of four monosaccharides, namely arabinose, mannose, galactose and xylose with the molar ratio of 15.59:1.52:4.76:1.0.
26277748	6	44	contain	has	1293:1295	arg2	potential					1305:1313	immense potential	1297:1313	immense potential	1297:1313	The in vitro test indicated that AAPF and AAPP have good antioxidant activity, demonstrating that A. auricula polysaccharide has immense potential as a functional food.
26277748	6	44	contain	has	1293:1295	arg1	polysaccharide					1278:1291	A. auricula polysaccharide	1266:1291	A. auricula polysaccharide	1266:1291	The in vitro test indicated that AAPF and AAPP have good antioxidant activity, demonstrating that A. auricula polysaccharide has immense potential as a functional food.
26277748	6	44	contain	has	1293:1295	arg2	food					1331:1334	a functional food	1318:1334	a functional food	1318:1334	The in vitro test indicated that AAPF and AAPP have good antioxidant activity, demonstrating that A. auricula polysaccharide has immense potential as a functional food.
26277748	3	45	with	mannose	585:591	arg1	ratio					620:624	a molar ratio	612:624	a molar ratio of 16.74:1.0:1.18:1.0:1.0	612:650	The results showed that AAPF consisted of five monosaccharides, including glucose, rhamnose, arabinose, mannose and galactose with a molar ratio of 16.74:1.0:1.18:1.0:1.0; in contrast, AAPP was composed of four monosaccharides, namely arabinose, mannose, galactose and xylose with the molar ratio of 15.59:1.52:4.76:1.0.
26277748	6	46	theme	A.	1266:1267	arg1	polysaccharide					1278:1291	A. auricula polysaccharide	1266:1291	A. auricula polysaccharide	1266:1291	The in vitro test indicated that AAPF and AAPP have good antioxidant activity, demonstrating that A. auricula polysaccharide has immense potential as a functional food.
26277748	3	47	theme	16.74:1.0:1.18:1.0:1.0	629:650	arg1	ratio					620:624	a molar ratio	612:624	a molar ratio of 16.74:1.0:1.18:1.0:1.0	612:650	The results showed that AAPF consisted of five monosaccharides, including glucose, rhamnose, arabinose, mannose and galactose with a molar ratio of 16.74:1.0:1.18:1.0:1.0; in contrast, AAPP was composed of four monosaccharides, namely arabinose, mannose, galactose and xylose with the molar ratio of 15.59:1.52:4.76:1.0.
26277748	3	48	with	mannose	727:733	arg1	ratio					772:776	the molar ratio	762:776	the molar ratio of 15.59:1.52:4.76:1.0	762:799	The results showed that AAPF consisted of five monosaccharides, including glucose, rhamnose, arabinose, mannose and galactose with a molar ratio of 16.74:1.0:1.18:1.0:1.0; in contrast, AAPP was composed of four monosaccharides, namely arabinose, mannose, galactose and xylose with the molar ratio of 15.59:1.52:4.76:1.0.
26277748	4	49	from	peaks	902:906	arg1	region					973:978	the carbohydrate region	956:978	the carbohydrate region	956:978	AAPF and AAPP were characterized by Fourier Transform Infrared spectroscopy (FTIR), which indicated peaks at approximately 3500cm(-1) and 522.28cm(-1) in the carbohydrate region and composed of β-glycoside linkages at the 1200-1000cm(-1) peaks.
26277748	6	50	theme	in	1172:1173	arg1	test					1181:1184	The in vitro test	1168:1184	The in vitro test	1168:1184	The in vitro test indicated that AAPF and AAPP have good antioxidant activity, demonstrating that A. auricula polysaccharide has immense potential as a functional food.
26277748	4	51	from	peaks	1040:1044	arg1	linkages					1008:1015	β-glycoside linkages	996:1015	β-glycoside linkages at the 1200-1000cm(-1) peaks	996:1044	AAPF and AAPP were characterized by Fourier Transform Infrared spectroscopy (FTIR), which indicated peaks at approximately 3500cm(-1) and 522.28cm(-1) in the carbohydrate region and composed of β-glycoside linkages at the 1200-1000cm(-1) peaks.
26277748	1	52	from	characterization	206:221	arg1	bodies					236:241	the fruit bodies	226:241	the fruit bodies of Auricularia auricula and pickled product	226:285	The study planned to determine proximate composition, antioxidant activity and chemical characterization in the fruit bodies of Auricularia auricula and pickled product.
26277748	1	53	theme	proximate	149:157	arg1	composition					159:169	proximate composition	149:169	proximate composition	149:169	The study planned to determine proximate composition, antioxidant activity and chemical characterization in the fruit bodies of Auricularia auricula and pickled product.
26277748	3	54	with	galactose	736:744	arg1	ratio					772:776	the molar ratio	762:776	the molar ratio of 15.59:1.52:4.76:1.0	762:799	The results showed that AAPF consisted of five monosaccharides, including glucose, rhamnose, arabinose, mannose and galactose with a molar ratio of 16.74:1.0:1.18:1.0:1.0; in contrast, AAPP was composed of four monosaccharides, namely arabinose, mannose, galactose and xylose with the molar ratio of 15.59:1.52:4.76:1.0.
26277748	1	55	theme	auricula	258:265	arg1	bodies					236:241	the fruit bodies	226:241	the fruit bodies of Auricularia auricula and pickled product	226:285	The study planned to determine proximate composition, antioxidant activity and chemical characterization in the fruit bodies of Auricularia auricula and pickled product.
26277748	0	56	theme	properties	77:86	arg1	Characterization					0:15	Characterization	0:15	Characterization of Auricularia auricula polysaccharides and its antioxidant properties in fresh and pickled product.	0:116	Characterization of Auricularia auricula polysaccharides and its antioxidant properties in fresh and pickled product.
26277748	5	57	theme	AAPF	1084:1087	arg1	activity					1072:1079	the antioxidant activity	1056:1079	the antioxidant activity of AAPF	1056:1087	However, the antioxidant activity of AAPF demonstrated a significant result in scavenging free radicals except for DDPH.
26277748	1	58	from	activity	184:191	arg1	bodies					236:241	the fruit bodies	226:241	the fruit bodies of Auricularia auricula and pickled product	226:285	The study planned to determine proximate composition, antioxidant activity and chemical characterization in the fruit bodies of Auricularia auricula and pickled product.
26277748	6	59	dep	A.	1266:1267	arg1	auricula					1269:1276	auricula	1269:1276	auricula	1269:1276	The in vitro test indicated that AAPF and AAPP have good antioxidant activity, demonstrating that A. auricula polysaccharide has immense potential as a functional food.
28332409	0	0	theme	chitosan	95:102	arg1	composites					104:113	chitosan composites	95:113	chitosan composites	95:113	Laboratory and pilot-scale field experiments for application of iron oxide nanoparticle-loaded chitosan composites to phosphate removal from natural water.
28332409	6	1	theme	adsorption	1265:1274	arg1	tower					1276:1280	the adsorption tower	1261:1280	the adsorption tower (height = 100 cm, inner diameter = 45 cm, flow rate = 7.05 ± 0.18 L/min) for phosphate removal	1261:1375	The natural water was pumped from the Seoho Stream into the pilot plant, passed through the chemical reactor/dissolved air flotation process, and then introduced into the adsorption tower (height = 100 cm, inner diameter = 45 cm, flow rate = 7.05 ± 0.18 L/min) for phosphate removal via the composites (composite volume = 80 L, composite weight = 85.74 kg).
28332409	6	2	theme	inner	1300:1304	arg1	height = 100 cm					1283:1297	height = 100 cm	1283:1297	height = 100 cm	1283:1297	The natural water was pumped from the Seoho Stream into the pilot plant, passed through the chemical reactor/dissolved air flotation process, and then introduced into the adsorption tower (height = 100 cm, inner diameter = 45 cm, flow rate = 7.05 ± 0.18 L/min) for phosphate removal via the composites (composite volume = 80 L, composite weight = 85.74 kg).
28332409	6	2	theme	inner	1300:1304	arg1	diameter = 45 cm					1306:1321	inner diameter = 45 cm	1300:1321	inner diameter = 45 cm	1300:1321	The natural water was pumped from the Seoho Stream into the pilot plant, passed through the chemical reactor/dissolved air flotation process, and then introduced into the adsorption tower (height = 100 cm, inner diameter = 45 cm, flow rate = 7.05 ± 0.18 L/min) for phosphate removal via the composites (composite volume = 80 L, composite weight = 85.74 kg).
28332409	2	3	theme	Laboratory	351:360	arg1	experiments					368:378	Laboratory batch experiments	351:378	Laboratory batch experiments	351:378	Laboratory batch experiments showed that phosphate removal by the ION-chitosan composites was not sensitive to pH changes between pH values of 5.0 and 9.0.
28332409	7	4	theme	0.010-0.028 mgP/L	1653:1669	arg1	range					1644:1648	the range	1640:1648	the range of 0.010-0.028 mgP/L	1640:1669	During monitoring of the adsorption tower (33 days), the influent total phosphorus (T-P) concentration was in the range of 0.020-0.046 mgP/L, whereas the effluent T-P concentration was in the range of 0.010-0.028 mgP/L.
28332409	1	5	theme	phosphate	253:261	arg1	removal					263:269	phosphate removal	253:269	phosphate removal from natural water collected from the Seoho Stream in Suwon, Republic of Korea	253:348	The aim of this study was to apply iron oxide nanoparticle-chitosan (ION-chitosan) composites to phosphate removal from natural water collected from the Seoho Stream in Suwon, Republic of Korea.
28332409	6	6	theme	composite	1397:1405	arg1	volume = 80 L					1407:1419	composite volume = 80 L	1397:1419	composite volume = 80 L	1397:1419	The natural water was pumped from the Seoho Stream into the pilot plant, passed through the chemical reactor/dissolved air flotation process, and then introduced into the adsorption tower (height = 100 cm, inner diameter = 45 cm, flow rate = 7.05 ± 0.18 L/min) for phosphate removal via the composites (composite volume = 80 L, composite weight = 85.74 kg).
28332409	6	6	theme	composite	1397:1405	arg1	weight = 85.74 kg					1432:1448	composite weight = 85.74 kg	1422:1448	composite weight = 85.74 kg	1422:1448	The natural water was pumped from the Seoho Stream into the pilot plant, passed through the chemical reactor/dissolved air flotation process, and then introduced into the adsorption tower (height = 100 cm, inner diameter = 45 cm, flow rate = 7.05 ± 0.18 L/min) for phosphate removal via the composites (composite volume = 80 L, composite weight = 85.74 kg).
28332409	4	7	theme	flow	888:891	arg1	conditions					893:902	dynamic flow conditions	880:902	dynamic flow conditions	880:902	Laboratory fixed-bed column experiments (column height = 10 and 20 cm, inner diameter = 2.5 cm, flow rate = 8.18 and 16.36 mL/min) demonstrated that the composites could be successfully applied for phosphate removal under dynamic flow conditions.
28332409	3	8	theme	phosphate	639:647	arg1	removal					649:655	phosphate removal	639:655	phosphate removal	639:655	During six cycles of adsorption-desorption, the composites could be successfully regenerated with 5 mM NaOH solution and reused for phosphate removal.
28332409	7	9	theme	0.020-0.046 mgP/L	1575:1591	arg1	range					1566:1570	the range	1562:1570	the range of 0.020-0.046 mgP/L	1562:1591	During monitoring of the adsorption tower (33 days), the influent total phosphorus (T-P) concentration was in the range of 0.020-0.046 mgP/L, whereas the effluent T-P concentration was in the range of 0.010-0.028 mgP/L.
28332409	6	10	theme	flotation	1217:1225	arg1	process					1227:1233	the chemical reactor/dissolved air flotation process	1182:1233	the chemical reactor/dissolved air flotation process	1182:1233	The natural water was pumped from the Seoho Stream into the pilot plant, passed through the chemical reactor/dissolved air flotation process, and then introduced into the adsorption tower (height = 100 cm, inner diameter = 45 cm, flow rate = 7.05 ± 0.18 L/min) for phosphate removal via the composites (composite volume = 80 L, composite weight = 85.74 kg).
28332409	3	11	theme	adsorption-desorption	528:548	arg1	cycles					518:523	six cycles	514:523	six cycles of adsorption-desorption	514:548	During six cycles of adsorption-desorption, the composites could be successfully regenerated with 5 mM NaOH solution and reused for phosphate removal.
28332409	6	12	dep	tower	1276:1280	arg1	height = 100 cm					1283:1297	height = 100 cm	1283:1297	height = 100 cm	1283:1297	The natural water was pumped from the Seoho Stream into the pilot plant, passed through the chemical reactor/dissolved air flotation process, and then introduced into the adsorption tower (height = 100 cm, inner diameter = 45 cm, flow rate = 7.05 ± 0.18 L/min) for phosphate removal via the composites (composite volume = 80 L, composite weight = 85.74 kg).
28332409	6	12	dep	tower	1276:1280	arg1	diameter = 45 cm					1306:1321	inner diameter = 45 cm	1300:1321	inner diameter = 45 cm	1300:1321	The natural water was pumped from the Seoho Stream into the pilot plant, passed through the chemical reactor/dissolved air flotation process, and then introduced into the adsorption tower (height = 100 cm, inner diameter = 45 cm, flow rate = 7.05 ± 0.18 L/min) for phosphate removal via the composites (composite volume = 80 L, composite weight = 85.74 kg).
28332409	6	12	dep	tower	1276:1280	arg1	rate = 7.05 ± 0.18 L/min					1329:1352	flow rate = 7.05 ± 0.18 L/min	1324:1352	flow rate = 7.05 ± 0.18 L/min	1324:1352	The natural water was pumped from the Seoho Stream into the pilot plant, passed through the chemical reactor/dissolved air flotation process, and then introduced into the adsorption tower (height = 100 cm, inner diameter = 45 cm, flow rate = 7.05 ± 0.18 L/min) for phosphate removal via the composites (composite volume = 80 L, composite weight = 85.74 kg).
28332409	6	13	dep	composites	1385:1394	arg1	volume = 80 L					1407:1419	composite volume = 80 L	1397:1419	composite volume = 80 L	1397:1419	The natural water was pumped from the Seoho Stream into the pilot plant, passed through the chemical reactor/dissolved air flotation process, and then introduced into the adsorption tower (height = 100 cm, inner diameter = 45 cm, flow rate = 7.05 ± 0.18 L/min) for phosphate removal via the composites (composite volume = 80 L, composite weight = 85.74 kg).
28332409	6	13	dep	composites	1385:1394	arg1	weight = 85.74 kg					1432:1448	composite weight = 85.74 kg	1422:1448	composite weight = 85.74 kg	1422:1448	The natural water was pumped from the Seoho Stream into the pilot plant, passed through the chemical reactor/dissolved air flotation process, and then introduced into the adsorption tower (height = 100 cm, inner diameter = 45 cm, flow rate = 7.05 ± 0.18 L/min) for phosphate removal via the composites (composite volume = 80 L, composite weight = 85.74 kg).
28332409	4	14	theme	16.36	775:779	arg1	mL/min					781:786	16.36 mL/min	775:786	16.36 mL/min	775:786	Laboratory fixed-bed column experiments (column height = 10 and 20 cm, inner diameter = 2.5 cm, flow rate = 8.18 and 16.36 mL/min) demonstrated that the composites could be successfully applied for phosphate removal under dynamic flow conditions.
28332409	4	14	theme	16.36	775:779	arg1	experiments					686:696	Laboratory fixed-bed column experiments	658:696	Laboratory fixed-bed column experiments (column height = 10 and 20 cm, inner diameter = 2.5 cm, flow rate = 8.18 and 16.36 mL/min)	658:787	Laboratory fixed-bed column experiments (column height = 10 and 20 cm, inner diameter = 2.5 cm, flow rate = 8.18 and 16.36 mL/min) demonstrated that the composites could be successfully applied for phosphate removal under dynamic flow conditions.
28332409	1	15	theme	natural	276:282	arg1	water					284:288	natural water	276:288	natural water collected from the Seoho Stream in Suwon, Republic of Korea	276:348	The aim of this study was to apply iron oxide nanoparticle-chitosan (ION-chitosan) composites to phosphate removal from natural water collected from the Seoho Stream in Suwon, Republic of Korea.
28332409	2	16	theme	ION-chitosan	417:428	arg1	composites					430:439	the ION-chitosan composites	413:439	the ION-chitosan composites	413:439	Laboratory batch experiments showed that phosphate removal by the ION-chitosan composites was not sensitive to pH changes between pH values of 5.0 and 9.0.
28332409	8	17	theme	adsorption	1706:1715	arg1	tower					1717:1721	the adsorption tower	1702:1721	the adsorption tower	1702:1721	The percent removal of T-P in the adsorption tower was 52.3% with a phosphate removal capacity of 0.059 mgP/g.
28332409	1	18	theme	study	172:176	arg1	aim					160:162	The aim	156:162	The aim of this study	156:176	The aim of this study was to apply iron oxide nanoparticle-chitosan (ION-chitosan) composites to phosphate removal from natural water collected from the Seoho Stream in Suwon, Republic of Korea.
28332409	0	19	theme	phosphate	118:126	arg1	removal					128:134	phosphate removal	118:134	phosphate removal from natural water	118:153	Laboratory and pilot-scale field experiments for application of iron oxide nanoparticle-loaded chitosan composites to phosphate removal from natural water.
28332409	7	20	theme	tower	1488:1492	arg1	monitoring					1459:1468	monitoring	1459:1468	monitoring of the adsorption tower (33 days)	1459:1502	During monitoring of the adsorption tower (33 days), the influent total phosphorus (T-P) concentration was in the range of 0.020-0.046 mgP/L, whereas the effluent T-P concentration was in the range of 0.010-0.028 mgP/L.
28332409	4	21	theme	phosphate	856:864	arg1	removal					866:872	phosphate removal	856:872	phosphate removal	856:872	Laboratory fixed-bed column experiments (column height = 10 and 20 cm, inner diameter = 2.5 cm, flow rate = 8.18 and 16.36 mL/min) demonstrated that the composites could be successfully applied for phosphate removal under dynamic flow conditions.
28332409	8	22	from	removal	1684:1690	arg1	tower					1717:1721	the adsorption tower	1702:1721	the adsorption tower	1702:1721	The percent removal of T-P in the adsorption tower was 52.3% with a phosphate removal capacity of 0.059 mgP/g.
28332409	5	23	theme	chemical	997:1004	arg1	flotation					1028:1036	chemical reactor/dissolved air flotation	997:1036	chemical reactor/dissolved air flotation	997:1036	A pilot-scale field experiment was performed in a pilot plant, which was mainly composed of chemical reactor/dissolved air flotation and an adsorption tower, built nearby the Seoho Stream.
28332409	7	24	theme	total	1518:1522	arg1	concentration					1541:1553	the influent total phosphorus (T-P) concentration	1505:1553	the influent total phosphorus (T-P) concentration	1505:1553	During monitoring of the adsorption tower (33 days), the influent total phosphorus (T-P) concentration was in the range of 0.020-0.046 mgP/L, whereas the effluent T-P concentration was in the range of 0.010-0.028 mgP/L.
28332409	2	25	theme	phosphate	392:400	arg1	removal					402:408	phosphate removal	392:408	phosphate removal by the ION-chitosan composites	392:439	Laboratory batch experiments showed that phosphate removal by the ION-chitosan composites was not sensitive to pH changes between pH values of 5.0 and 9.0.
28332409	1	26	from	water	284:288	arg1	removal					263:269	phosphate removal	253:269	phosphate removal from natural water collected from the Seoho Stream in Suwon, Republic of Korea	253:348	The aim of this study was to apply iron oxide nanoparticle-chitosan (ION-chitosan) composites to phosphate removal from natural water collected from the Seoho Stream in Suwon, Republic of Korea.
28332409	6	27	theme	composite	1422:1430	arg1	volume = 80 L					1407:1419	composite volume = 80 L	1397:1419	composite volume = 80 L	1397:1419	The natural water was pumped from the Seoho Stream into the pilot plant, passed through the chemical reactor/dissolved air flotation process, and then introduced into the adsorption tower (height = 100 cm, inner diameter = 45 cm, flow rate = 7.05 ± 0.18 L/min) for phosphate removal via the composites (composite volume = 80 L, composite weight = 85.74 kg).
28332409	6	27	theme	composite	1422:1430	arg1	weight = 85.74 kg					1432:1448	composite weight = 85.74 kg	1422:1448	composite weight = 85.74 kg	1422:1448	The natural water was pumped from the Seoho Stream into the pilot plant, passed through the chemical reactor/dissolved air flotation process, and then introduced into the adsorption tower (height = 100 cm, inner diameter = 45 cm, flow rate = 7.05 ± 0.18 L/min) for phosphate removal via the composites (composite volume = 80 L, composite weight = 85.74 kg).
28332409	4	28	theme	flow	754:757	arg1	rate = 8.18					759:769	flow rate = 8.18	754:769	flow rate = 8.18	754:769	Laboratory fixed-bed column experiments (column height = 10 and 20 cm, inner diameter = 2.5 cm, flow rate = 8.18 and 16.36 mL/min) demonstrated that the composites could be successfully applied for phosphate removal under dynamic flow conditions.
28332409	4	28	theme	flow	754:757	arg1	experiments					686:696	Laboratory fixed-bed column experiments	658:696	Laboratory fixed-bed column experiments (column height = 10 and 20 cm, inner diameter = 2.5 cm, flow rate = 8.18 and 16.36 mL/min)	658:787	Laboratory fixed-bed column experiments (column height = 10 and 20 cm, inner diameter = 2.5 cm, flow rate = 8.18 and 16.36 mL/min) demonstrated that the composites could be successfully applied for phosphate removal under dynamic flow conditions.
28332409	0	29	theme	Laboratory	0:9	arg1	experiments					33:43	Laboratory and pilot-scale field experiments	0:43	Laboratory and pilot-scale field experiments for application of iron oxide	0:73	Laboratory and pilot-scale field experiments for application of iron oxide nanoparticle-loaded chitosan composites to phosphate removal from natural water.
28332409	5	30	theme	Seoho	1080:1084	arg1	tower					1056:1060	an adsorption tower	1042:1060	an adsorption tower	1042:1060	A pilot-scale field experiment was performed in a pilot plant, which was mainly composed of chemical reactor/dissolved air flotation and an adsorption tower, built nearby the Seoho Stream.
28332409	5	30	theme	Seoho	1080:1084	arg1	Stream					1086:1091	the Seoho Stream	1076:1091	built nearby the Seoho Stream	1063:1091	A pilot-scale field experiment was performed in a pilot plant, which was mainly composed of chemical reactor/dissolved air flotation and an adsorption tower, built nearby the Seoho Stream.
28332409	7	31	theme	influent	1509:1516	arg1	concentration					1541:1553	the influent total phosphorus (T-P) concentration	1505:1553	the influent total phosphorus (T-P) concentration	1505:1553	During monitoring of the adsorption tower (33 days), the influent total phosphorus (T-P) concentration was in the range of 0.020-0.046 mgP/L, whereas the effluent T-P concentration was in the range of 0.010-0.028 mgP/L.
28332409	6	32	theme	natural	1098:1104	arg1	water					1106:1110	The natural water	1094:1110	The natural water	1094:1110	The natural water was pumped from the Seoho Stream into the pilot plant, passed through the chemical reactor/dissolved air flotation process, and then introduced into the adsorption tower (height = 100 cm, inner diameter = 45 cm, flow rate = 7.05 ± 0.18 L/min) for phosphate removal via the composites (composite volume = 80 L, composite weight = 85.74 kg).
28332409	4	33	theme	column	679:684	arg1	diameter = 2.5 cm					735:751	inner diameter = 2.5 cm	729:751	inner diameter = 2.5 cm	729:751	Laboratory fixed-bed column experiments (column height = 10 and 20 cm, inner diameter = 2.5 cm, flow rate = 8.18 and 16.36 mL/min) demonstrated that the composites could be successfully applied for phosphate removal under dynamic flow conditions.
28332409	4	33	theme	column	679:684	arg1	mL/min					781:786	16.36 mL/min	775:786	16.36 mL/min	775:786	Laboratory fixed-bed column experiments (column height = 10 and 20 cm, inner diameter = 2.5 cm, flow rate = 8.18 and 16.36 mL/min) demonstrated that the composites could be successfully applied for phosphate removal under dynamic flow conditions.
28332409	4	33	theme	column	679:684	arg1	height = 10					706:716	column height = 10	699:716	column height = 10	699:716	Laboratory fixed-bed column experiments (column height = 10 and 20 cm, inner diameter = 2.5 cm, flow rate = 8.18 and 16.36 mL/min) demonstrated that the composites could be successfully applied for phosphate removal under dynamic flow conditions.
28332409	4	33	theme	column	679:684	arg1	rate = 8.18					759:769	flow rate = 8.18	754:769	flow rate = 8.18	754:769	Laboratory fixed-bed column experiments (column height = 10 and 20 cm, inner diameter = 2.5 cm, flow rate = 8.18 and 16.36 mL/min) demonstrated that the composites could be successfully applied for phosphate removal under dynamic flow conditions.
28332409	4	33	theme	column	679:684	arg1	20 cm					722:726	20 cm	722:726	20 cm	722:726	Laboratory fixed-bed column experiments (column height = 10 and 20 cm, inner diameter = 2.5 cm, flow rate = 8.18 and 16.36 mL/min) demonstrated that the composites could be successfully applied for phosphate removal under dynamic flow conditions.
28332409	4	33	theme	column	679:684	arg1	experiments					686:696	Laboratory fixed-bed column experiments	658:696	Laboratory fixed-bed column experiments (column height = 10 and 20 cm, inner diameter = 2.5 cm, flow rate = 8.18 and 16.36 mL/min)	658:787	Laboratory fixed-bed column experiments (column height = 10 and 20 cm, inner diameter = 2.5 cm, flow rate = 8.18 and 16.36 mL/min) demonstrated that the composites could be successfully applied for phosphate removal under dynamic flow conditions.
28332409	0	34	theme	field	27:31	arg1	experiments					33:43	Laboratory and pilot-scale field experiments	0:43	Laboratory and pilot-scale field experiments for application of iron oxide	0:73	Laboratory and pilot-scale field experiments for application of iron oxide nanoparticle-loaded chitosan composites to phosphate removal from natural water.
28332409	6	35	theme	air	1213:1215	arg1	process					1227:1233	the chemical reactor/dissolved air flotation process	1182:1233	the chemical reactor/dissolved air flotation process	1182:1233	The natural water was pumped from the Seoho Stream into the pilot plant, passed through the chemical reactor/dissolved air flotation process, and then introduced into the adsorption tower (height = 100 cm, inner diameter = 45 cm, flow rate = 7.05 ± 0.18 L/min) for phosphate removal via the composites (composite volume = 80 L, composite weight = 85.74 kg).
28332409	0	36	theme	natural	141:147	arg1	water					149:153	natural water	141:153	natural water	141:153	Laboratory and pilot-scale field experiments for application of iron oxide nanoparticle-loaded chitosan composites to phosphate removal from natural water.
28332409	5	37	theme	pilot	955:959	arg1	plant					961:965	a pilot plant	953:965	a pilot plant	953:965	A pilot-scale field experiment was performed in a pilot plant, which was mainly composed of chemical reactor/dissolved air flotation and an adsorption tower, built nearby the Seoho Stream.
28332409	6	38	theme	pilot	1154:1158	arg1	plant					1160:1164	the pilot plant	1150:1164	the pilot plant	1150:1164	The natural water was pumped from the Seoho Stream into the pilot plant, passed through the chemical reactor/dissolved air flotation process, and then introduced into the adsorption tower (height = 100 cm, inner diameter = 45 cm, flow rate = 7.05 ± 0.18 L/min) for phosphate removal via the composites (composite volume = 80 L, composite weight = 85.74 kg).
28332409	8	39	with	%	1731:1731	arg1	capacity					1758:1765	a phosphate removal capacity	1738:1765	a phosphate removal capacity of 0.059 mgP/g	1738:1780	The percent removal of T-P in the adsorption tower was 52.3% with a phosphate removal capacity of 0.059 mgP/g.
28332409	1	40	theme	iron	191:194	arg1	ION-chitosan					225:236	ION-chitosan	225:236	ION-chitosan	225:236	The aim of this study was to apply iron oxide nanoparticle-chitosan (ION-chitosan) composites to phosphate removal from natural water collected from the Seoho Stream in Suwon, Republic of Korea.
28332409	1	40	theme	iron	191:194	arg1	nanoparticle-chitosan					202:222	iron oxide nanoparticle-chitosan	191:222	iron oxide nanoparticle-chitosan (ION-chitosan) composites	191:248	The aim of this study was to apply iron oxide nanoparticle-chitosan (ION-chitosan) composites to phosphate removal from natural water collected from the Seoho Stream in Suwon, Republic of Korea.
28332409	0	41	theme	pilot-scale	15:25	arg1	experiments					33:43	Laboratory and pilot-scale field experiments	0:43	Laboratory and pilot-scale field experiments for application of iron oxide	0:73	Laboratory and pilot-scale field experiments for application of iron oxide nanoparticle-loaded chitosan composites to phosphate removal from natural water.
28332409	6	42	theme	phosphate	1359:1367	arg1	removal					1369:1375	phosphate removal	1359:1375	phosphate removal	1359:1375	The natural water was pumped from the Seoho Stream into the pilot plant, passed through the chemical reactor/dissolved air flotation process, and then introduced into the adsorption tower (height = 100 cm, inner diameter = 45 cm, flow rate = 7.05 ± 0.18 L/min) for phosphate removal via the composites (composite volume = 80 L, composite weight = 85.74 kg).
28332409	6	43	theme	chemical	1186:1193	arg1	process					1227:1233	the chemical reactor/dissolved air flotation process	1182:1233	the chemical reactor/dissolved air flotation process	1182:1233	The natural water was pumped from the Seoho Stream into the pilot plant, passed through the chemical reactor/dissolved air flotation process, and then introduced into the adsorption tower (height = 100 cm, inner diameter = 45 cm, flow rate = 7.05 ± 0.18 L/min) for phosphate removal via the composites (composite volume = 80 L, composite weight = 85.74 kg).
28332409	8	44	theme	phosphate	1740:1748	arg1	capacity					1758:1765	a phosphate removal capacity	1738:1765	a phosphate removal capacity of 0.059 mgP/g	1738:1780	The percent removal of T-P in the adsorption tower was 52.3% with a phosphate removal capacity of 0.059 mgP/g.
28332409	1	45	theme	oxide	196:200	arg1	ION-chitosan					225:236	ION-chitosan	225:236	ION-chitosan	225:236	The aim of this study was to apply iron oxide nanoparticle-chitosan (ION-chitosan) composites to phosphate removal from natural water collected from the Seoho Stream in Suwon, Republic of Korea.
28332409	1	45	theme	oxide	196:200	arg1	nanoparticle-chitosan					202:222	iron oxide nanoparticle-chitosan	191:222	iron oxide nanoparticle-chitosan (ION-chitosan) composites	191:248	The aim of this study was to apply iron oxide nanoparticle-chitosan (ION-chitosan) composites to phosphate removal from natural water collected from the Seoho Stream in Suwon, Republic of Korea.
28332409	2	46	theme	pH	481:482	arg1	values					484:489	pH values	481:489	pH values of 5.0 and 9.0	481:504	Laboratory batch experiments showed that phosphate removal by the ION-chitosan composites was not sensitive to pH changes between pH values of 5.0 and 9.0.
28332409	7	47	theme	adsorption	1477:1486	arg1	tower					1488:1492	the adsorption tower	1473:1492	the adsorption tower (33 days)	1473:1502	During monitoring of the adsorption tower (33 days), the influent total phosphorus (T-P) concentration was in the range of 0.020-0.046 mgP/L, whereas the effluent T-P concentration was in the range of 0.010-0.028 mgP/L.
28332409	7	47	theme	adsorption	1477:1486	arg1	days					1498:1501	33 days	1495:1501	33 days	1495:1501	During monitoring of the adsorption tower (33 days), the influent total phosphorus (T-P) concentration was in the range of 0.020-0.046 mgP/L, whereas the effluent T-P concentration was in the range of 0.010-0.028 mgP/L.
28332409	7	48	theme	T-P	1536:1538	arg1	concentration					1541:1553	the influent total phosphorus (T-P) concentration	1505:1553	the influent total phosphorus (T-P) concentration	1505:1553	During monitoring of the adsorption tower (33 days), the influent total phosphorus (T-P) concentration was in the range of 0.020-0.046 mgP/L, whereas the effluent T-P concentration was in the range of 0.010-0.028 mgP/L.
28332409	1	49	theme	nanoparticle-chitosan	202:222	arg1	composites					239:248	iron oxide nanoparticle-chitosan (ION-chitosan) composites	191:248	iron oxide nanoparticle-chitosan (ION-chitosan) composites	191:248	The aim of this study was to apply iron oxide nanoparticle-chitosan (ION-chitosan) composites to phosphate removal from natural water collected from the Seoho Stream in Suwon, Republic of Korea.
28332409	4	50	theme	fixed-bed	669:677	arg1	diameter = 2.5 cm					735:751	inner diameter = 2.5 cm	729:751	inner diameter = 2.5 cm	729:751	Laboratory fixed-bed column experiments (column height = 10 and 20 cm, inner diameter = 2.5 cm, flow rate = 8.18 and 16.36 mL/min) demonstrated that the composites could be successfully applied for phosphate removal under dynamic flow conditions.
28332409	4	50	theme	fixed-bed	669:677	arg1	mL/min					781:786	16.36 mL/min	775:786	16.36 mL/min	775:786	Laboratory fixed-bed column experiments (column height = 10 and 20 cm, inner diameter = 2.5 cm, flow rate = 8.18 and 16.36 mL/min) demonstrated that the composites could be successfully applied for phosphate removal under dynamic flow conditions.
28332409	4	50	theme	fixed-bed	669:677	arg1	height = 10					706:716	column height = 10	699:716	column height = 10	699:716	Laboratory fixed-bed column experiments (column height = 10 and 20 cm, inner diameter = 2.5 cm, flow rate = 8.18 and 16.36 mL/min) demonstrated that the composites could be successfully applied for phosphate removal under dynamic flow conditions.
28332409	4	50	theme	fixed-bed	669:677	arg1	rate = 8.18					759:769	flow rate = 8.18	754:769	flow rate = 8.18	754:769	Laboratory fixed-bed column experiments (column height = 10 and 20 cm, inner diameter = 2.5 cm, flow rate = 8.18 and 16.36 mL/min) demonstrated that the composites could be successfully applied for phosphate removal under dynamic flow conditions.
28332409	4	50	theme	fixed-bed	669:677	arg1	20 cm					722:726	20 cm	722:726	20 cm	722:726	Laboratory fixed-bed column experiments (column height = 10 and 20 cm, inner diameter = 2.5 cm, flow rate = 8.18 and 16.36 mL/min) demonstrated that the composites could be successfully applied for phosphate removal under dynamic flow conditions.
28332409	4	50	theme	fixed-bed	669:677	arg1	experiments					686:696	Laboratory fixed-bed column experiments	658:696	Laboratory fixed-bed column experiments (column height = 10 and 20 cm, inner diameter = 2.5 cm, flow rate = 8.18 and 16.36 mL/min)	658:787	Laboratory fixed-bed column experiments (column height = 10 and 20 cm, inner diameter = 2.5 cm, flow rate = 8.18 and 16.36 mL/min) demonstrated that the composites could be successfully applied for phosphate removal under dynamic flow conditions.
28332409	1	51	theme	Seoho	309:313	arg1	Stream					315:320	the Seoho Stream	305:320	the Seoho Stream	305:320	The aim of this study was to apply iron oxide nanoparticle-chitosan (ION-chitosan) composites to phosphate removal from natural water collected from the Seoho Stream in Suwon, Republic of Korea.
28332409	3	52	theme	5 mM	605:608	arg1	solution					615:622	5 mM NaOH solution	605:622	5 mM NaOH solution	605:622	During six cycles of adsorption-desorption, the composites could be successfully regenerated with 5 mM NaOH solution and reused for phosphate removal.
28332409	2	53	theme	9.0	502:504	arg1	values					484:489	pH values	481:489	pH values of 5.0 and 9.0	481:504	Laboratory batch experiments showed that phosphate removal by the ION-chitosan composites was not sensitive to pH changes between pH values of 5.0 and 9.0.
28332409	7	54	theme	effluent	1606:1613	arg1	concentration					1619:1631	the effluent T-P concentration	1602:1631	the effluent T-P concentration	1602:1631	During monitoring of the adsorption tower (33 days), the influent total phosphorus (T-P) concentration was in the range of 0.020-0.046 mgP/L, whereas the effluent T-P concentration was in the range of 0.010-0.028 mgP/L.
28332409	5	55	theme	pilot-scale	907:917	arg1	experiment					925:934	A pilot-scale field experiment	905:934	A pilot-scale field experiment	905:934	A pilot-scale field experiment was performed in a pilot plant, which was mainly composed of chemical reactor/dissolved air flotation and an adsorption tower, built nearby the Seoho Stream.
28332409	2	56	theme	5.0	494:496	arg1	values					484:489	pH values	481:489	pH values of 5.0 and 9.0	481:504	Laboratory batch experiments showed that phosphate removal by the ION-chitosan composites was not sensitive to pH changes between pH values of 5.0 and 9.0.
28332409	8	57	theme	removal	1750:1756	arg1	capacity					1758:1765	a phosphate removal capacity	1738:1765	a phosphate removal capacity of 0.059 mgP/g	1738:1780	The percent removal of T-P in the adsorption tower was 52.3% with a phosphate removal capacity of 0.059 mgP/g.
28332409	8	58	attach	removal	1684:1690	arg2	T-P					1695:1697	T-P	1695:1697	T-P	1695:1697	The percent removal of T-P in the adsorption tower was 52.3% with a phosphate removal capacity of 0.059 mgP/g.
28332409	8	58	attach	removal	1684:1690	arg3	tower					1717:1721	the adsorption tower	1702:1721	the adsorption tower	1702:1721	The percent removal of T-P in the adsorption tower was 52.3% with a phosphate removal capacity of 0.059 mgP/g.
28332409	2	59	theme	pH	462:463	arg1	changes					465:471	pH changes	462:471	pH changes between pH values of 5.0 and 9.0	462:504	Laboratory batch experiments showed that phosphate removal by the ION-chitosan composites was not sensitive to pH changes between pH values of 5.0 and 9.0.
28332409	7	60	theme	T-P	1615:1617	arg1	concentration					1619:1631	the effluent T-P concentration	1602:1631	the effluent T-P concentration	1602:1631	During monitoring of the adsorption tower (33 days), the influent total phosphorus (T-P) concentration was in the range of 0.020-0.046 mgP/L, whereas the effluent T-P concentration was in the range of 0.010-0.028 mgP/L.
28332409	6	61	theme	flow	1324:1327	arg1	height = 100 cm					1283:1297	height = 100 cm	1283:1297	height = 100 cm	1283:1297	The natural water was pumped from the Seoho Stream into the pilot plant, passed through the chemical reactor/dissolved air flotation process, and then introduced into the adsorption tower (height = 100 cm, inner diameter = 45 cm, flow rate = 7.05 ± 0.18 L/min) for phosphate removal via the composites (composite volume = 80 L, composite weight = 85.74 kg).
28332409	6	61	theme	flow	1324:1327	arg1	rate = 7.05 ± 0.18 L/min					1329:1352	flow rate = 7.05 ± 0.18 L/min	1324:1352	flow rate = 7.05 ± 0.18 L/min	1324:1352	The natural water was pumped from the Seoho Stream into the pilot plant, passed through the chemical reactor/dissolved air flotation process, and then introduced into the adsorption tower (height = 100 cm, inner diameter = 45 cm, flow rate = 7.05 ± 0.18 L/min) for phosphate removal via the composites (composite volume = 80 L, composite weight = 85.74 kg).
28332409	5	62	theme	nearby	1069:1074	arg1	tower					1056:1060	an adsorption tower	1042:1060	an adsorption tower	1042:1060	A pilot-scale field experiment was performed in a pilot plant, which was mainly composed of chemical reactor/dissolved air flotation and an adsorption tower, built nearby the Seoho Stream.
28332409	5	62	theme	nearby	1069:1074	arg1	Stream					1086:1091	the Seoho Stream	1076:1091	built nearby the Seoho Stream	1063:1091	A pilot-scale field experiment was performed in a pilot plant, which was mainly composed of chemical reactor/dissolved air flotation and an adsorption tower, built nearby the Seoho Stream.
28332409	8	63	theme	0.059 mgP/g	1770:1780	arg1	capacity					1758:1765	a phosphate removal capacity	1738:1765	a phosphate removal capacity of 0.059 mgP/g	1738:1780	The percent removal of T-P in the adsorption tower was 52.3% with a phosphate removal capacity of 0.059 mgP/g.
28332409	5	64	theme	reactor/dissolved	1006:1022	arg1	flotation					1028:1036	chemical reactor/dissolved air flotation	997:1036	chemical reactor/dissolved air flotation	997:1036	A pilot-scale field experiment was performed in a pilot plant, which was mainly composed of chemical reactor/dissolved air flotation and an adsorption tower, built nearby the Seoho Stream.
28332409	4	65	dep	experiments	686:696	arg1	diameter = 2.5 cm					735:751	inner diameter = 2.5 cm	729:751	inner diameter = 2.5 cm	729:751	Laboratory fixed-bed column experiments (column height = 10 and 20 cm, inner diameter = 2.5 cm, flow rate = 8.18 and 16.36 mL/min) demonstrated that the composites could be successfully applied for phosphate removal under dynamic flow conditions.
28332409	4	65	dep	experiments	686:696	arg1	mL/min					781:786	16.36 mL/min	775:786	16.36 mL/min	775:786	Laboratory fixed-bed column experiments (column height = 10 and 20 cm, inner diameter = 2.5 cm, flow rate = 8.18 and 16.36 mL/min) demonstrated that the composites could be successfully applied for phosphate removal under dynamic flow conditions.
28332409	4	65	dep	experiments	686:696	arg1	height = 10					706:716	column height = 10	699:716	column height = 10	699:716	Laboratory fixed-bed column experiments (column height = 10 and 20 cm, inner diameter = 2.5 cm, flow rate = 8.18 and 16.36 mL/min) demonstrated that the composites could be successfully applied for phosphate removal under dynamic flow conditions.
28332409	4	65	dep	experiments	686:696	arg1	rate = 8.18					759:769	flow rate = 8.18	754:769	flow rate = 8.18	754:769	Laboratory fixed-bed column experiments (column height = 10 and 20 cm, inner diameter = 2.5 cm, flow rate = 8.18 and 16.36 mL/min) demonstrated that the composites could be successfully applied for phosphate removal under dynamic flow conditions.
28332409	4	65	dep	experiments	686:696	arg1	20 cm					722:726	20 cm	722:726	20 cm	722:726	Laboratory fixed-bed column experiments (column height = 10 and 20 cm, inner diameter = 2.5 cm, flow rate = 8.18 and 16.36 mL/min) demonstrated that the composites could be successfully applied for phosphate removal under dynamic flow conditions.
28332409	4	65	dep	experiments	686:696	arg1	experiments					686:696	Laboratory fixed-bed column experiments	658:696	Laboratory fixed-bed column experiments (column height = 10 and 20 cm, inner diameter = 2.5 cm, flow rate = 8.18 and 16.36 mL/min)	658:787	Laboratory fixed-bed column experiments (column height = 10 and 20 cm, inner diameter = 2.5 cm, flow rate = 8.18 and 16.36 mL/min) demonstrated that the composites could be successfully applied for phosphate removal under dynamic flow conditions.
28332409	5	66	theme	adsorption	1045:1054	arg1	tower					1056:1060	an adsorption tower	1042:1060	an adsorption tower	1042:1060	A pilot-scale field experiment was performed in a pilot plant, which was mainly composed of chemical reactor/dissolved air flotation and an adsorption tower, built nearby the Seoho Stream.
28332409	5	66	theme	adsorption	1045:1054	arg1	Stream					1086:1091	the Seoho Stream	1076:1091	built nearby the Seoho Stream	1063:1091	A pilot-scale field experiment was performed in a pilot plant, which was mainly composed of chemical reactor/dissolved air flotation and an adsorption tower, built nearby the Seoho Stream.
28332409	6	67	theme	Seoho	1132:1136	arg1	Stream					1138:1143	the Seoho Stream	1128:1143	the Seoho Stream into the pilot plant	1128:1164	The natural water was pumped from the Seoho Stream into the pilot plant, passed through the chemical reactor/dissolved air flotation process, and then introduced into the adsorption tower (height = 100 cm, inner diameter = 45 cm, flow rate = 7.05 ± 0.18 L/min) for phosphate removal via the composites (composite volume = 80 L, composite weight = 85.74 kg).
28332409	8	68	theme	percent	1676:1682	arg1	removal					1684:1690	The percent removal	1672:1690	The percent removal of T-P in the adsorption tower	1672:1721	The percent removal of T-P in the adsorption tower was 52.3% with a phosphate removal capacity of 0.059 mgP/g.
28332409	8	68	theme	percent	1676:1682	arg1	%					1731:1731	52.3%	1727:1731	52.3%	1727:1731	The percent removal of T-P in the adsorption tower was 52.3% with a phosphate removal capacity of 0.059 mgP/g.
28332409	4	69	theme	dynamic	880:886	arg1	conditions					893:902	dynamic flow conditions	880:902	dynamic flow conditions	880:902	Laboratory fixed-bed column experiments (column height = 10 and 20 cm, inner diameter = 2.5 cm, flow rate = 8.18 and 16.36 mL/min) demonstrated that the composites could be successfully applied for phosphate removal under dynamic flow conditions.
28332409	5	70	theme	air	1024:1026	arg1	flotation					1028:1036	chemical reactor/dissolved air flotation	997:1036	chemical reactor/dissolved air flotation	997:1036	A pilot-scale field experiment was performed in a pilot plant, which was mainly composed of chemical reactor/dissolved air flotation and an adsorption tower, built nearby the Seoho Stream.
28332409	4	71	theme	inner	729:733	arg1	experiments					686:696	Laboratory fixed-bed column experiments	658:696	Laboratory fixed-bed column experiments (column height = 10 and 20 cm, inner diameter = 2.5 cm, flow rate = 8.18 and 16.36 mL/min)	658:787	Laboratory fixed-bed column experiments (column height = 10 and 20 cm, inner diameter = 2.5 cm, flow rate = 8.18 and 16.36 mL/min) demonstrated that the composites could be successfully applied for phosphate removal under dynamic flow conditions.
28332409	4	71	theme	inner	729:733	arg1	diameter = 2.5 cm					735:751	inner diameter = 2.5 cm	729:751	inner diameter = 2.5 cm	729:751	Laboratory fixed-bed column experiments (column height = 10 and 20 cm, inner diameter = 2.5 cm, flow rate = 8.18 and 16.36 mL/min) demonstrated that the composites could be successfully applied for phosphate removal under dynamic flow conditions.
28332409	0	72	from	water	149:153	arg1	removal					128:134	phosphate removal	118:134	phosphate removal from natural water	118:153	Laboratory and pilot-scale field experiments for application of iron oxide nanoparticle-loaded chitosan composites to phosphate removal from natural water.
28332409	0	73	theme	oxide	69:73	arg1	application					49:59	application	49:59	application of iron oxide	49:73	Laboratory and pilot-scale field experiments for application of iron oxide nanoparticle-loaded chitosan composites to phosphate removal from natural water.
28332409	5	74	theme	field	919:923	arg1	experiment					925:934	A pilot-scale field experiment	905:934	A pilot-scale field experiment	905:934	A pilot-scale field experiment was performed in a pilot plant, which was mainly composed of chemical reactor/dissolved air flotation and an adsorption tower, built nearby the Seoho Stream.
28332409	3	75	theme	NaOH	610:613	arg1	solution					615:622	5 mM NaOH solution	605:622	5 mM NaOH solution	605:622	During six cycles of adsorption-desorption, the composites could be successfully regenerated with 5 mM NaOH solution and reused for phosphate removal.
28332409	4	76	theme	column	699:704	arg1	height = 10					706:716	column height = 10	699:716	column height = 10	699:716	Laboratory fixed-bed column experiments (column height = 10 and 20 cm, inner diameter = 2.5 cm, flow rate = 8.18 and 16.36 mL/min) demonstrated that the composites could be successfully applied for phosphate removal under dynamic flow conditions.
28332409	4	76	theme	column	699:704	arg1	experiments					686:696	Laboratory fixed-bed column experiments	658:696	Laboratory fixed-bed column experiments (column height = 10 and 20 cm, inner diameter = 2.5 cm, flow rate = 8.18 and 16.36 mL/min)	658:787	Laboratory fixed-bed column experiments (column height = 10 and 20 cm, inner diameter = 2.5 cm, flow rate = 8.18 and 16.36 mL/min) demonstrated that the composites could be successfully applied for phosphate removal under dynamic flow conditions.
28332409	4	77	theme	Laboratory	658:667	arg1	diameter = 2.5 cm					735:751	inner diameter = 2.5 cm	729:751	inner diameter = 2.5 cm	729:751	Laboratory fixed-bed column experiments (column height = 10 and 20 cm, inner diameter = 2.5 cm, flow rate = 8.18 and 16.36 mL/min) demonstrated that the composites could be successfully applied for phosphate removal under dynamic flow conditions.
28332409	4	77	theme	Laboratory	658:667	arg1	mL/min					781:786	16.36 mL/min	775:786	16.36 mL/min	775:786	Laboratory fixed-bed column experiments (column height = 10 and 20 cm, inner diameter = 2.5 cm, flow rate = 8.18 and 16.36 mL/min) demonstrated that the composites could be successfully applied for phosphate removal under dynamic flow conditions.
28332409	4	77	theme	Laboratory	658:667	arg1	height = 10					706:716	column height = 10	699:716	column height = 10	699:716	Laboratory fixed-bed column experiments (column height = 10 and 20 cm, inner diameter = 2.5 cm, flow rate = 8.18 and 16.36 mL/min) demonstrated that the composites could be successfully applied for phosphate removal under dynamic flow conditions.
28332409	4	77	theme	Laboratory	658:667	arg1	rate = 8.18					759:769	flow rate = 8.18	754:769	flow rate = 8.18	754:769	Laboratory fixed-bed column experiments (column height = 10 and 20 cm, inner diameter = 2.5 cm, flow rate = 8.18 and 16.36 mL/min) demonstrated that the composites could be successfully applied for phosphate removal under dynamic flow conditions.
28332409	4	77	theme	Laboratory	658:667	arg1	20 cm					722:726	20 cm	722:726	20 cm	722:726	Laboratory fixed-bed column experiments (column height = 10 and 20 cm, inner diameter = 2.5 cm, flow rate = 8.18 and 16.36 mL/min) demonstrated that the composites could be successfully applied for phosphate removal under dynamic flow conditions.
28332409	4	77	theme	Laboratory	658:667	arg1	experiments					686:696	Laboratory fixed-bed column experiments	658:696	Laboratory fixed-bed column experiments (column height = 10 and 20 cm, inner diameter = 2.5 cm, flow rate = 8.18 and 16.36 mL/min)	658:787	Laboratory fixed-bed column experiments (column height = 10 and 20 cm, inner diameter = 2.5 cm, flow rate = 8.18 and 16.36 mL/min) demonstrated that the composites could be successfully applied for phosphate removal under dynamic flow conditions.
28332409	0	78	theme	iron	64:67	arg1	oxide					69:73	iron oxide	64:73	iron oxide	64:73	Laboratory and pilot-scale field experiments for application of iron oxide nanoparticle-loaded chitosan composites to phosphate removal from natural water.
28332409	6	79	theme	reactor/dissolved	1195:1211	arg1	process					1227:1233	the chemical reactor/dissolved air flotation process	1182:1233	the chemical reactor/dissolved air flotation process	1182:1233	The natural water was pumped from the Seoho Stream into the pilot plant, passed through the chemical reactor/dissolved air flotation process, and then introduced into the adsorption tower (height = 100 cm, inner diameter = 45 cm, flow rate = 7.05 ± 0.18 L/min) for phosphate removal via the composites (composite volume = 80 L, composite weight = 85.74 kg).
28332409	2	80	theme	batch	362:366	arg1	experiments					368:378	Laboratory batch experiments	351:378	Laboratory batch experiments	351:378	Laboratory batch experiments showed that phosphate removal by the ION-chitosan composites was not sensitive to pH changes between pH values of 5.0 and 9.0.
28332409	8	81	theme	T-P	1695:1697	arg1	removal					1684:1690	The percent removal	1672:1690	The percent removal of T-P in the adsorption tower	1672:1721	The percent removal of T-P in the adsorption tower was 52.3% with a phosphate removal capacity of 0.059 mgP/g.
28332409	8	81	theme	T-P	1695:1697	arg1	%					1731:1731	52.3%	1727:1731	52.3%	1727:1731	The percent removal of T-P in the adsorption tower was 52.3% with a phosphate removal capacity of 0.059 mgP/g.
28332409	7	82	theme	phosphorus	1524:1533	arg1	concentration					1541:1553	the influent total phosphorus (T-P) concentration	1505:1553	the influent total phosphorus (T-P) concentration	1505:1553	During monitoring of the adsorption tower (33 days), the influent total phosphorus (T-P) concentration was in the range of 0.020-0.046 mgP/L, whereas the effluent T-P concentration was in the range of 0.010-0.028 mgP/L.
28708979	11	0	theme	antibodies	2157:2166	arg1	"					2136:2136	the "tolerance"	2122:2136	the "tolerance" of natural anti-Pk antibodies towards cell-bound Gb3Cer	2122:2192	CONCLUSIONS Both immunochemical and molecular dynamics data argue that the reason of the "tolerance" of natural anti-Pk antibodies towards cell-bound Gb3Cer is the spatial inaccessibility of Pk glycotope for interaction.
28708979	4	1	theme	cognate	622:628	arg1	antigen					630:636	their cognate antigen	616:636	their cognate antigen	616:636	Intriguingly, the same trisaccharide is a key fragment of glycosphingolipid globotriaosylceramide (Gb3Cer) - normal component of erythrocyte and endothelial cell membrane, i.e. the antibodies and their cognate antigen coexist without any immunological reaction.
28708979	9	2	theme	synthetic	1496:1504	arg1	analogue					1506:1513	synthetic analogue	1496:1513	synthetic analogue differing by the presence of a spacer between trisaccharide and lipid part	1496:1588	RESULTS Isolated specific antibodies were completely unable to bind natural Gb3Cer both inserted into cells and in artificial membrane, whereas strong interaction took place with synthetic analogue differing by the presence of a spacer between trisaccharide and lipid part.
28708979	11	3	theme	natural	2141:2147	arg1	antibodies					2157:2166	natural anti-Pk antibodies	2141:2166	natural anti-Pk antibodies	2141:2166	CONCLUSIONS Both immunochemical and molecular dynamics data argue that the reason of the "tolerance" of natural anti-Pk antibodies towards cell-bound Gb3Cer is the spatial inaccessibility of Pk glycotope for interaction.
28708979	5	4	theme	human	714:718	arg1	antibodies					728:737	human anti-Pk antibodies	714:737	human anti-Pk antibodies	714:737	AIM To explain the inertness of human anti-Pk antibodies towards own cells.
28708979	6	5	theme	experiments	848:858	arg1	combination					790:800	a combination	788:800	a combination of immunochemical and molecular dynamics (MD) experiments	788:858	MATERIALS AND METHODS We used a combination of immunochemical and molecular dynamics (MD) experiments.
28708979	10	6	theme	glycan	1819:1824	arg1	accessibility					1967:1979	better accessibility	1960:1979	iv) better accessibility of the synthetic version for interaction with proteins	1956:2034	MD simulations revealed: i) although membrane-bound glycans do not form stable long-living aggregates, their transient packing is more compact in natural Gb3 as compared with the synthetic analog, ii) similar conformation of Pk glycan in composition of the glycolipids, iii) no effect on the mentioned above results when cholesterol was inserted into membrane, and iv) better accessibility of the synthetic version for interaction with proteins.
28708979	10	6	theme	glycan	1819:1824	arg1	effect					1869:1874	no effect	1866:1874	iii) no effect on the mentioned above results when cholesterol was inserted into membrane	1861:1949	MD simulations revealed: i) although membrane-bound glycans do not form stable long-living aggregates, their transient packing is more compact in natural Gb3 as compared with the synthetic analog, ii) similar conformation of Pk glycan in composition of the glycolipids, iii) no effect on the mentioned above results when cholesterol was inserted into membrane, and iv) better accessibility of the synthetic version for interaction with proteins.
28708979	10	6	theme	glycan	1819:1824	arg1	conformation					1800:1811	ii) similar conformation	1788:1811	ii) similar conformation of Pk glycan in composition of the glycolipids	1788:1858	MD simulations revealed: i) although membrane-bound glycans do not form stable long-living aggregates, their transient packing is more compact in natural Gb3 as compared with the synthetic analog, ii) similar conformation of Pk glycan in composition of the glycolipids, iii) no effect on the mentioned above results when cholesterol was inserted into membrane, and iv) better accessibility of the synthetic version for interaction with proteins.
28708979	7	7	theme	Pk	912:913	arg1	trisaccharide					915:927	Pk trisaccharide	912:927	Pk trisaccharide	912:927	Antibodies were isolated using affinity media with Pk trisaccharide, their epitope specificity was characterized using ELISA (enzyme-linked immunosorbent assay) with a set of synthetic glycans related to Pk synthetic glycans and FACS (Fluorescence-Activated Cell Sorting) analysis of cells with inserted natural Gb3Cer and its synthetic analogue.
28708979	8	8	theme	glycolipids	1240:1250	arg1	clustering					1226:1235	clustering	1226:1235	clustering	1226:1235	Conformations and clustering of glycolipids immersed into a lipid bilayer were studied using MD simulations.
28708979	8	8	theme	glycolipids	1240:1250	arg1	Conformations					1208:1220	Conformations	1208:1220	Conformations	1208:1220	Conformations and clustering of glycolipids immersed into a lipid bilayer were studied using MD simulations.
28708979	4	9	theme	same	438:441	arg1	fragment					466:473	a key fragment	460:473	a key fragment of glycosphingolipid globotriaosylceramide (Gb3Cer) - normal component of erythrocyte and endothelial cell membrane, i.e. the antibodies and their cognate antigen coexist without any immunological reaction	460:679	Intriguingly, the same trisaccharide is a key fragment of glycosphingolipid globotriaosylceramide (Gb3Cer) - normal component of erythrocyte and endothelial cell membrane, i.e. the antibodies and their cognate antigen coexist without any immunological reaction.
28708979	4	9	theme	same	438:441	arg1	trisaccharide					443:455	the same trisaccharide	434:455	the same trisaccharide	434:455	Intriguingly, the same trisaccharide is a key fragment of glycosphingolipid globotriaosylceramide (Gb3Cer) - normal component of erythrocyte and endothelial cell membrane, i.e. the antibodies and their cognate antigen coexist without any immunological reaction.
28708979	2	10	theme	Human	176:180	arg1	blood					182:186	BACKGROUND Human blood	165:186	BACKGROUND Human blood	165:186	BACKGROUND Human blood contains a big variety of natural antibodies, circulating throughout life at constant concentration.
28708979	11	11	theme	Pk	2228:2229	arg1	glycotope					2231:2239	Pk glycotope	2228:2239	Pk glycotope for interaction	2228:2255	CONCLUSIONS Both immunochemical and molecular dynamics data argue that the reason of the "tolerance" of natural anti-Pk antibodies towards cell-bound Gb3Cer is the spatial inaccessibility of Pk glycotope for interaction.
28708979	7	12	with	ELISA	980:984	arg1	glycans					1046:1052	synthetic glycans	1036:1052	synthetic glycans related to Pk synthetic glycans	1036:1084	Antibodies were isolated using affinity media with Pk trisaccharide, their epitope specificity was characterized using ELISA (enzyme-linked immunosorbent assay) with a set of synthetic glycans related to Pk synthetic glycans and FACS (Fluorescence-Activated Cell Sorting) analysis of cells with inserted natural Gb3Cer and its synthetic analogue.
28708979	7	12	with	ELISA	980:984	arg1	set					1029:1031	a set	1027:1031	a set of synthetic glycans related to Pk synthetic glycans	1027:1084	Antibodies were isolated using affinity media with Pk trisaccharide, their epitope specificity was characterized using ELISA (enzyme-linked immunosorbent assay) with a set of synthetic glycans related to Pk synthetic glycans and FACS (Fluorescence-Activated Cell Sorting) analysis of cells with inserted natural Gb3Cer and its synthetic analogue.
28708979	5	13	theme	antibodies	728:737	arg1	inertness					701:709	the inertness	697:709	the inertness of human anti-Pk antibodies towards own cells	697:755	AIM To explain the inertness of human anti-Pk antibodies towards own cells.
28708979	7	14	theme	cells	1145:1149	arg1	assay					1015:1019	enzyme-linked immunosorbent assay	987:1019	enzyme-linked immunosorbent assay	987:1019	Antibodies were isolated using affinity media with Pk trisaccharide, their epitope specificity was characterized using ELISA (enzyme-linked immunosorbent assay) with a set of synthetic glycans related to Pk synthetic glycans and FACS (Fluorescence-Activated Cell Sorting) analysis of cells with inserted natural Gb3Cer and its synthetic analogue.
28708979	7	14	theme	cells	1145:1149	arg1	analysis					1133:1140	FACS (Fluorescence-Activated Cell Sorting) analysis	1090:1140	FACS (Fluorescence-Activated Cell Sorting) analysis of cells with inserted natural Gb3Cer and its synthetic analogue	1090:1205	Antibodies were isolated using affinity media with Pk trisaccharide, their epitope specificity was characterized using ELISA (enzyme-linked immunosorbent assay) with a set of synthetic glycans related to Pk synthetic glycans and FACS (Fluorescence-Activated Cell Sorting) analysis of cells with inserted natural Gb3Cer and its synthetic analogue.
28708979	7	14	theme	cells	1145:1149	arg1	ELISA					980:984	ELISA	980:984	ELISA (enzyme-linked immunosorbent assay) with a set of synthetic glycans related to Pk synthetic glycans	980:1084	Antibodies were isolated using affinity media with Pk trisaccharide, their epitope specificity was characterized using ELISA (enzyme-linked immunosorbent assay) with a set of synthetic glycans related to Pk synthetic glycans and FACS (Fluorescence-Activated Cell Sorting) analysis of cells with inserted natural Gb3Cer and its synthetic analogue.
28708979	6	15	theme	dynamics	834:841	arg1	experiments					848:858	immunochemical and molecular dynamics (MD) experiments	805:858	immunochemical and molecular dynamics (MD) experiments	805:858	MATERIALS AND METHODS We used a combination of immunochemical and molecular dynamics (MD) experiments.
28708979	8	16	theme	lipid	1268:1272	arg1	bilayer					1274:1280	a lipid bilayer	1266:1280	a lipid bilayer	1266:1280	Conformations and clustering of glycolipids immersed into a lipid bilayer were studied using MD simulations.
28708979	11	17	dep	immunochemical	2054:2067	arg1	data					2092:2095	data	2092:2095	data	2092:2095	CONCLUSIONS Both immunochemical and molecular dynamics data argue that the reason of the "tolerance" of natural anti-Pk antibodies towards cell-bound Gb3Cer is the spatial inaccessibility of Pk glycotope for interaction.
28708979	10	18	theme	stable	1663:1668	arg1	aggregates					1682:1691	stable long-living aggregates	1663:1691	stable long-living aggregates	1663:1691	MD simulations revealed: i) although membrane-bound glycans do not form stable long-living aggregates, their transient packing is more compact in natural Gb3 as compared with the synthetic analog, ii) similar conformation of Pk glycan in composition of the glycolipids, iii) no effect on the mentioned above results when cholesterol was inserted into membrane, and iv) better accessibility of the synthetic version for interaction with proteins.
28708979	10	19	theme	version	1998:2004	arg1	accessibility					1967:1979	better accessibility	1960:1979	iv) better accessibility of the synthetic version for interaction with proteins	1956:2034	MD simulations revealed: i) although membrane-bound glycans do not form stable long-living aggregates, their transient packing is more compact in natural Gb3 as compared with the synthetic analog, ii) similar conformation of Pk glycan in composition of the glycolipids, iii) no effect on the mentioned above results when cholesterol was inserted into membrane, and iv) better accessibility of the synthetic version for interaction with proteins.
28708979	10	19	theme	version	1998:2004	arg1	effect					1869:1874	no effect	1866:1874	iii) no effect on the mentioned above results when cholesterol was inserted into membrane	1861:1949	MD simulations revealed: i) although membrane-bound glycans do not form stable long-living aggregates, their transient packing is more compact in natural Gb3 as compared with the synthetic analog, ii) similar conformation of Pk glycan in composition of the glycolipids, iii) no effect on the mentioned above results when cholesterol was inserted into membrane, and iv) better accessibility of the synthetic version for interaction with proteins.
28708979	10	19	theme	version	1998:2004	arg1	conformation					1800:1811	ii) similar conformation	1788:1811	ii) similar conformation of Pk glycan in composition of the glycolipids	1788:1858	MD simulations revealed: i) although membrane-bound glycans do not form stable long-living aggregates, their transient packing is more compact in natural Gb3 as compared with the synthetic analog, ii) similar conformation of Pk glycan in composition of the glycolipids, iii) no effect on the mentioned above results when cholesterol was inserted into membrane, and iv) better accessibility of the synthetic version for interaction with proteins.
28708979	9	20	theme	spacer	1546:1551	arg1	presence					1532:1539	the presence	1528:1539	the presence of a spacer between trisaccharide and lipid part	1528:1588	RESULTS Isolated specific antibodies were completely unable to bind natural Gb3Cer both inserted into cells and in artificial membrane, whereas strong interaction took place with synthetic analogue differing by the presence of a spacer between trisaccharide and lipid part.
28708979	10	21	from	accessibility	1967:1979	arg1	results					1899:1905	the mentioned above results	1879:1905	the mentioned above results when cholesterol was inserted into membrane	1879:1949	MD simulations revealed: i) although membrane-bound glycans do not form stable long-living aggregates, their transient packing is more compact in natural Gb3 as compared with the synthetic analog, ii) similar conformation of Pk glycan in composition of the glycolipids, iii) no effect on the mentioned above results when cholesterol was inserted into membrane, and iv) better accessibility of the synthetic version for interaction with proteins.
28708979	10	21	from	accessibility	1967:1979	arg1	composition					1829:1839	composition	1829:1839	composition of the glycolipids	1829:1858	MD simulations revealed: i) although membrane-bound glycans do not form stable long-living aggregates, their transient packing is more compact in natural Gb3 as compared with the synthetic analog, ii) similar conformation of Pk glycan in composition of the glycolipids, iii) no effect on the mentioned above results when cholesterol was inserted into membrane, and iv) better accessibility of the synthetic version for interaction with proteins.
28708979	1	22	theme	immunochemical	135:148	arg1	investigation					150:162	immunochemical investigation	135:162	immunochemical investigation	135:162	Molecular dynamics and immunochemical investigation.
28708979	7	23	theme	synthetic	1068:1076	arg1	glycans					1078:1084	Pk synthetic glycans	1065:1084	Pk synthetic glycans	1065:1084	Antibodies were isolated using affinity media with Pk trisaccharide, their epitope specificity was characterized using ELISA (enzyme-linked immunosorbent assay) with a set of synthetic glycans related to Pk synthetic glycans and FACS (Fluorescence-Activated Cell Sorting) analysis of cells with inserted natural Gb3Cer and its synthetic analogue.
28708979	11	24	dep	CONCLUSIONS	2037:2047	arg1	argue					2097:2101	argue	2097:2101	argue that the reason of the "tolerance" of natural anti-Pk antibodies towards cell-bound Gb3Cer is the spatial inaccessibility of Pk glycotope for interaction	2097:2255	CONCLUSIONS Both immunochemical and molecular dynamics data argue that the reason of the "tolerance" of natural anti-Pk antibodies towards cell-bound Gb3Cer is the spatial inaccessibility of Pk glycotope for interaction.
28708979	11	25	theme	"	2136:2136	arg1	reason					2112:2117	the reason	2108:2117	the reason of the "tolerance" of natural anti-Pk antibodies towards cell-bound Gb3Cer	2108:2192	CONCLUSIONS Both immunochemical and molecular dynamics data argue that the reason of the "tolerance" of natural anti-Pk antibodies towards cell-bound Gb3Cer is the spatial inaccessibility of Pk glycotope for interaction.
28708979	11	25	theme	"	2136:2136	arg1	inaccessibility					2209:2223	the spatial inaccessibility	2197:2223	the spatial inaccessibility of Pk glycotope for interaction	2197:2255	CONCLUSIONS Both immunochemical and molecular dynamics data argue that the reason of the "tolerance" of natural anti-Pk antibodies towards cell-bound Gb3Cer is the spatial inaccessibility of Pk glycotope for interaction.
28708979	4	26	theme	globotriaosylceramide	496:516	arg1	fragment					466:473	a key fragment	460:473	a key fragment of glycosphingolipid globotriaosylceramide (Gb3Cer) - normal component of erythrocyte and endothelial cell membrane, i.e. the antibodies and their cognate antigen coexist without any immunological reaction	460:679	Intriguingly, the same trisaccharide is a key fragment of glycosphingolipid globotriaosylceramide (Gb3Cer) - normal component of erythrocyte and endothelial cell membrane, i.e. the antibodies and their cognate antigen coexist without any immunological reaction.
28708979	4	26	theme	globotriaosylceramide	496:516	arg1	trisaccharide					443:455	the same trisaccharide	434:455	the same trisaccharide	434:455	Intriguingly, the same trisaccharide is a key fragment of glycosphingolipid globotriaosylceramide (Gb3Cer) - normal component of erythrocyte and endothelial cell membrane, i.e. the antibodies and their cognate antigen coexist without any immunological reaction.
28708979	10	27	with	interaction	2010:2020	arg1	proteins					2027:2034	proteins	2027:2034	proteins	2027:2034	MD simulations revealed: i) although membrane-bound glycans do not form stable long-living aggregates, their transient packing is more compact in natural Gb3 as compared with the synthetic analog, ii) similar conformation of Pk glycan in composition of the glycolipids, iii) no effect on the mentioned above results when cholesterol was inserted into membrane, and iv) better accessibility of the synthetic version for interaction with proteins.
28708979	7	28	theme	Fluorescence-Activated	1096:1117	arg1	Sorting					1124:1130	Fluorescence-Activated Cell Sorting	1096:1130	Fluorescence-Activated Cell Sorting	1096:1130	Antibodies were isolated using affinity media with Pk trisaccharide, their epitope specificity was characterized using ELISA (enzyme-linked immunosorbent assay) with a set of synthetic glycans related to Pk synthetic glycans and FACS (Fluorescence-Activated Cell Sorting) analysis of cells with inserted natural Gb3Cer and its synthetic analogue.
28708979	7	28	theme	Fluorescence-Activated	1096:1117	arg1	FACS					1090:1093	FACS	1090:1093	FACS (Fluorescence-Activated Cell Sorting) analysis of cells with inserted natural Gb3Cer and its synthetic analogue	1090:1205	Antibodies were isolated using affinity media with Pk trisaccharide, their epitope specificity was characterized using ELISA (enzyme-linked immunosorbent assay) with a set of synthetic glycans related to Pk synthetic glycans and FACS (Fluorescence-Activated Cell Sorting) analysis of cells with inserted natural Gb3Cer and its synthetic analogue.
28708979	9	29	theme	lipid	1579:1583	arg1	part					1585:1588	lipid part	1579:1588	lipid part	1579:1588	RESULTS Isolated specific antibodies were completely unable to bind natural Gb3Cer both inserted into cells and in artificial membrane, whereas strong interaction took place with synthetic analogue differing by the presence of a spacer between trisaccharide and lipid part.
28708979	5	30	theme	own	747:749	arg1	cells					751:755	own cells	747:755	own cells	747:755	AIM To explain the inertness of human anti-Pk antibodies towards own cells.
28708979	7	31	theme	glycans	1046:1052	arg1	glycans					1046:1052	synthetic glycans	1036:1052	synthetic glycans related to Pk synthetic glycans	1036:1084	Antibodies were isolated using affinity media with Pk trisaccharide, their epitope specificity was characterized using ELISA (enzyme-linked immunosorbent assay) with a set of synthetic glycans related to Pk synthetic glycans and FACS (Fluorescence-Activated Cell Sorting) analysis of cells with inserted natural Gb3Cer and its synthetic analogue.
28708979	7	31	theme	glycans	1046:1052	arg1	set					1029:1031	a set	1027:1031	a set of synthetic glycans related to Pk synthetic glycans	1027:1084	Antibodies were isolated using affinity media with Pk trisaccharide, their epitope specificity was characterized using ELISA (enzyme-linked immunosorbent assay) with a set of synthetic glycans related to Pk synthetic glycans and FACS (Fluorescence-Activated Cell Sorting) analysis of cells with inserted natural Gb3Cer and its synthetic analogue.
28708979	4	32	theme	key	462:464	arg1	fragment					466:473	a key fragment	460:473	a key fragment of glycosphingolipid globotriaosylceramide (Gb3Cer) - normal component of erythrocyte and endothelial cell membrane, i.e. the antibodies and their cognate antigen coexist without any immunological reaction	460:679	Intriguingly, the same trisaccharide is a key fragment of glycosphingolipid globotriaosylceramide (Gb3Cer) - normal component of erythrocyte and endothelial cell membrane, i.e. the antibodies and their cognate antigen coexist without any immunological reaction.
28708979	4	32	theme	key	462:464	arg1	trisaccharide					443:455	the same trisaccharide	434:455	the same trisaccharide	434:455	Intriguingly, the same trisaccharide is a key fragment of glycosphingolipid globotriaosylceramide (Gb3Cer) - normal component of erythrocyte and endothelial cell membrane, i.e. the antibodies and their cognate antigen coexist without any immunological reaction.
28708979	4	33	theme	component	536:544	arg1	fragment					466:473	a key fragment	460:473	a key fragment of glycosphingolipid globotriaosylceramide (Gb3Cer) - normal component of erythrocyte and endothelial cell membrane, i.e. the antibodies and their cognate antigen coexist without any immunological reaction	460:679	Intriguingly, the same trisaccharide is a key fragment of glycosphingolipid globotriaosylceramide (Gb3Cer) - normal component of erythrocyte and endothelial cell membrane, i.e. the antibodies and their cognate antigen coexist without any immunological reaction.
28708979	4	33	theme	component	536:544	arg1	trisaccharide					443:455	the same trisaccharide	434:455	the same trisaccharide	434:455	Intriguingly, the same trisaccharide is a key fragment of glycosphingolipid globotriaosylceramide (Gb3Cer) - normal component of erythrocyte and endothelial cell membrane, i.e. the antibodies and their cognate antigen coexist without any immunological reaction.
28708979	7	34	theme	FACS	1090:1093	arg1	analysis					1133:1140	FACS (Fluorescence-Activated Cell Sorting) analysis	1090:1140	FACS (Fluorescence-Activated Cell Sorting) analysis of cells with inserted natural Gb3Cer and its synthetic analogue	1090:1205	Antibodies were isolated using affinity media with Pk trisaccharide, their epitope specificity was characterized using ELISA (enzyme-linked immunosorbent assay) with a set of synthetic glycans related to Pk synthetic glycans and FACS (Fluorescence-Activated Cell Sorting) analysis of cells with inserted natural Gb3Cer and its synthetic analogue.
28708979	2	35	contain	contains	188:195	arg2	antibodies					222:231	natural antibodies	214:231	natural antibodies	214:231	BACKGROUND Human blood contains a big variety of natural antibodies, circulating throughout life at constant concentration.
28708979	2	35	contain	contains	188:195	arg1	blood					182:186	BACKGROUND Human blood	165:186	BACKGROUND Human blood	165:186	BACKGROUND Human blood contains a big variety of natural antibodies, circulating throughout life at constant concentration.
28708979	2	35	contain	contains	188:195	arg2	variety					203:209	a big variety	197:209	a big variety of natural antibodies	197:231	BACKGROUND Human blood contains a big variety of natural antibodies, circulating throughout life at constant concentration.
28708979	4	36	theme	erythrocyte	549:559	arg1	membrane					582:589	erythrocyte and endothelial cell membrane	549:589	membrane	582:589	Intriguingly, the same trisaccharide is a key fragment of glycosphingolipid globotriaosylceramide (Gb3Cer) - normal component of erythrocyte and endothelial cell membrane, i.e. the antibodies and their cognate antigen coexist without any immunological reaction.
28708979	11	37	theme	molecular	2073:2081	arg1	dynamics					2083:2090	molecular dynamics	2073:2090	molecular dynamics	2073:2090	CONCLUSIONS Both immunochemical and molecular dynamics data argue that the reason of the "tolerance" of natural anti-Pk antibodies towards cell-bound Gb3Cer is the spatial inaccessibility of Pk glycotope for interaction.
28708979	10	38	theme	natural	1737:1743	arg1	Gb3					1745:1747	natural Gb3	1737:1747	natural Gb3	1737:1747	MD simulations revealed: i) although membrane-bound glycans do not form stable long-living aggregates, their transient packing is more compact in natural Gb3 as compared with the synthetic analog, ii) similar conformation of Pk glycan in composition of the glycolipids, iii) no effect on the mentioned above results when cholesterol was inserted into membrane, and iv) better accessibility of the synthetic version for interaction with proteins.
28708979	7	39	theme	immunosorbent	1001:1013	arg1	assay					1015:1019	enzyme-linked immunosorbent assay	987:1019	enzyme-linked immunosorbent assay	987:1019	Antibodies were isolated using affinity media with Pk trisaccharide, their epitope specificity was characterized using ELISA (enzyme-linked immunosorbent assay) with a set of synthetic glycans related to Pk synthetic glycans and FACS (Fluorescence-Activated Cell Sorting) analysis of cells with inserted natural Gb3Cer and its synthetic analogue.
28708979	7	39	theme	immunosorbent	1001:1013	arg1	ELISA					980:984	ELISA	980:984	ELISA (enzyme-linked immunosorbent assay) with a set of synthetic glycans related to Pk synthetic glycans	980:1084	Antibodies were isolated using affinity media with Pk trisaccharide, their epitope specificity was characterized using ELISA (enzyme-linked immunosorbent assay) with a set of synthetic glycans related to Pk synthetic glycans and FACS (Fluorescence-Activated Cell Sorting) analysis of cells with inserted natural Gb3Cer and its synthetic analogue.
28708979	11	40	theme	spatial	2201:2207	arg1	inaccessibility					2209:2223	the spatial inaccessibility	2197:2223	the spatial inaccessibility of Pk glycotope for interaction	2197:2255	CONCLUSIONS Both immunochemical and molecular dynamics data argue that the reason of the "tolerance" of natural anti-Pk antibodies towards cell-bound Gb3Cer is the spatial inaccessibility of Pk glycotope for interaction.
28708979	11	40	theme	spatial	2201:2207	arg1	reason					2112:2117	the reason	2108:2117	the reason of the "tolerance" of natural anti-Pk antibodies towards cell-bound Gb3Cer	2108:2192	CONCLUSIONS Both immunochemical and molecular dynamics data argue that the reason of the "tolerance" of natural anti-Pk antibodies towards cell-bound Gb3Cer is the spatial inaccessibility of Pk glycotope for interaction.
28708979	6	41	dep	MATERIALS	758:766	arg1	We					780:781	We	780:781	We	780:781	MATERIALS AND METHODS We used a combination of immunochemical and molecular dynamics (MD) experiments.
28708979	0	42	theme	anti-Galα1-4Galβ1-4Glc	10:31	arg1	antibodies					41:50	human anti-Galα1-4Galβ1-4Glc natural antibodies	4:50	human anti-Galα1-4Galβ1-4Glc natural antibodies	4:50	Why human anti-Galα1-4Galβ1-4Glc natural antibodies do not recognize the trisaccharide on erythrocyte membrane?
28708979	9	43	dep	unable	1370:1375	arg1	both					1400:1403	both	1400:1403	both	1400:1403	RESULTS Isolated specific antibodies were completely unable to bind natural Gb3Cer both inserted into cells and in artificial membrane, whereas strong interaction took place with synthetic analogue differing by the presence of a spacer between trisaccharide and lipid part.
28708979	9	43	dep	unable	1370:1375	arg1	whereas					1453:1459	whereas	1453:1459	whereas	1453:1459	RESULTS Isolated specific antibodies were completely unable to bind natural Gb3Cer both inserted into cells and in artificial membrane, whereas strong interaction took place with synthetic analogue differing by the presence of a spacer between trisaccharide and lipid part.
28708979	9	44	theme	artificial	1432:1441	arg1	membrane					1443:1450	artificial membrane	1432:1450	artificial membrane	1432:1450	RESULTS Isolated specific antibodies were completely unable to bind natural Gb3Cer both inserted into cells and in artificial membrane, whereas strong interaction took place with synthetic analogue differing by the presence of a spacer between trisaccharide and lipid part.
28708979	10	45	from	compact	1726:1732	arg1	Gb3					1745:1747	natural Gb3	1737:1747	natural Gb3	1737:1747	MD simulations revealed: i) although membrane-bound glycans do not form stable long-living aggregates, their transient packing is more compact in natural Gb3 as compared with the synthetic analog, ii) similar conformation of Pk glycan in composition of the glycolipids, iii) no effect on the mentioned above results when cholesterol was inserted into membrane, and iv) better accessibility of the synthetic version for interaction with proteins.
28708979	7	46	with	analysis	1133:1140	arg1	glycans					1046:1052	synthetic glycans	1036:1052	synthetic glycans related to Pk synthetic glycans	1036:1084	Antibodies were isolated using affinity media with Pk trisaccharide, their epitope specificity was characterized using ELISA (enzyme-linked immunosorbent assay) with a set of synthetic glycans related to Pk synthetic glycans and FACS (Fluorescence-Activated Cell Sorting) analysis of cells with inserted natural Gb3Cer and its synthetic analogue.
28708979	7	46	with	analysis	1133:1140	arg1	set					1029:1031	a set	1027:1031	a set of synthetic glycans related to Pk synthetic glycans	1027:1084	Antibodies were isolated using affinity media with Pk trisaccharide, their epitope specificity was characterized using ELISA (enzyme-linked immunosorbent assay) with a set of synthetic glycans related to Pk synthetic glycans and FACS (Fluorescence-Activated Cell Sorting) analysis of cells with inserted natural Gb3Cer and its synthetic analogue.
28708979	11	47	theme	glycotope	2231:2239	arg1	inaccessibility					2209:2223	the spatial inaccessibility	2197:2223	the spatial inaccessibility of Pk glycotope for interaction	2197:2255	CONCLUSIONS Both immunochemical and molecular dynamics data argue that the reason of the "tolerance" of natural anti-Pk antibodies towards cell-bound Gb3Cer is the spatial inaccessibility of Pk glycotope for interaction.
28708979	11	47	theme	glycotope	2231:2239	arg1	reason					2112:2117	the reason	2108:2117	the reason of the "tolerance" of natural anti-Pk antibodies towards cell-bound Gb3Cer	2108:2192	CONCLUSIONS Both immunochemical and molecular dynamics data argue that the reason of the "tolerance" of natural anti-Pk antibodies towards cell-bound Gb3Cer is the spatial inaccessibility of Pk glycotope for interaction.
28708979	4	48	theme	endothelial	565:575	arg1	membrane					582:589	erythrocyte and endothelial cell membrane	549:589	membrane	582:589	Intriguingly, the same trisaccharide is a key fragment of glycosphingolipid globotriaosylceramide (Gb3Cer) - normal component of erythrocyte and endothelial cell membrane, i.e. the antibodies and their cognate antigen coexist without any immunological reaction.
28708979	10	49	theme	above	1893:1897	arg1	results					1899:1905	the mentioned above results	1879:1905	the mentioned above results when cholesterol was inserted into membrane	1879:1949	MD simulations revealed: i) although membrane-bound glycans do not form stable long-living aggregates, their transient packing is more compact in natural Gb3 as compared with the synthetic analog, ii) similar conformation of Pk glycan in composition of the glycolipids, iii) no effect on the mentioned above results when cholesterol was inserted into membrane, and iv) better accessibility of the synthetic version for interaction with proteins.
28708979	6	50	theme	immunochemical	805:818	arg1	experiments					848:858	immunochemical and molecular dynamics (MD) experiments	805:858	immunochemical and molecular dynamics (MD) experiments	805:858	MATERIALS AND METHODS We used a combination of immunochemical and molecular dynamics (MD) experiments.
28708979	7	51	with	media	901:905	arg1	trisaccharide					915:927	Pk trisaccharide	912:927	Pk trisaccharide	912:927	Antibodies were isolated using affinity media with Pk trisaccharide, their epitope specificity was characterized using ELISA (enzyme-linked immunosorbent assay) with a set of synthetic glycans related to Pk synthetic glycans and FACS (Fluorescence-Activated Cell Sorting) analysis of cells with inserted natural Gb3Cer and its synthetic analogue.
28708979	7	52	with	cells	1145:1149	arg1	Gb3Cer					1173:1178	Gb3Cer	1173:1178	Gb3Cer	1173:1178	Antibodies were isolated using affinity media with Pk trisaccharide, their epitope specificity was characterized using ELISA (enzyme-linked immunosorbent assay) with a set of synthetic glycans related to Pk synthetic glycans and FACS (Fluorescence-Activated Cell Sorting) analysis of cells with inserted natural Gb3Cer and its synthetic analogue.
28708979	7	52	with	cells	1145:1149	arg1	analogue					1198:1205	its synthetic analogue	1184:1205	its synthetic analogue	1184:1205	Antibodies were isolated using affinity media with Pk trisaccharide, their epitope specificity was characterized using ELISA (enzyme-linked immunosorbent assay) with a set of synthetic glycans related to Pk synthetic glycans and FACS (Fluorescence-Activated Cell Sorting) analysis of cells with inserted natural Gb3Cer and its synthetic analogue.
28708979	4	53	theme	membrane	582:589	arg1	component					536:544	normal component	529:544	normal component of erythrocyte and endothelial cell membrane, i.e. the antibodies and their cognate antigen coexist without any immunological reaction	529:679	Intriguingly, the same trisaccharide is a key fragment of glycosphingolipid globotriaosylceramide (Gb3Cer) - normal component of erythrocyte and endothelial cell membrane, i.e. the antibodies and their cognate antigen coexist without any immunological reaction.
28708979	10	54	dep	compact	1726:1732	arg1	accessibility					1967:1979	better accessibility	1960:1979	iv) better accessibility of the synthetic version for interaction with proteins	1956:2034	MD simulations revealed: i) although membrane-bound glycans do not form stable long-living aggregates, their transient packing is more compact in natural Gb3 as compared with the synthetic analog, ii) similar conformation of Pk glycan in composition of the glycolipids, iii) no effect on the mentioned above results when cholesterol was inserted into membrane, and iv) better accessibility of the synthetic version for interaction with proteins.
28708979	10	54	dep	compact	1726:1732	arg1	effect					1869:1874	no effect	1866:1874	iii) no effect on the mentioned above results when cholesterol was inserted into membrane	1861:1949	MD simulations revealed: i) although membrane-bound glycans do not form stable long-living aggregates, their transient packing is more compact in natural Gb3 as compared with the synthetic analog, ii) similar conformation of Pk glycan in composition of the glycolipids, iii) no effect on the mentioned above results when cholesterol was inserted into membrane, and iv) better accessibility of the synthetic version for interaction with proteins.
28708979	10	54	dep	compact	1726:1732	arg1	conformation					1800:1811	ii) similar conformation	1788:1811	ii) similar conformation of Pk glycan in composition of the glycolipids	1788:1858	MD simulations revealed: i) although membrane-bound glycans do not form stable long-living aggregates, their transient packing is more compact in natural Gb3 as compared with the synthetic analog, ii) similar conformation of Pk glycan in composition of the glycolipids, iii) no effect on the mentioned above results when cholesterol was inserted into membrane, and iv) better accessibility of the synthetic version for interaction with proteins.
28708979	10	55	theme	synthetic	1770:1778	arg1	analog					1780:1785	the synthetic analog	1766:1785	the synthetic analog	1766:1785	MD simulations revealed: i) although membrane-bound glycans do not form stable long-living aggregates, their transient packing is more compact in natural Gb3 as compared with the synthetic analog, ii) similar conformation of Pk glycan in composition of the glycolipids, iii) no effect on the mentioned above results when cholesterol was inserted into membrane, and iv) better accessibility of the synthetic version for interaction with proteins.
28708979	9	56	theme	strong	1461:1466	arg1	interaction					1468:1478	strong interaction	1461:1478	strong interaction	1461:1478	RESULTS Isolated specific antibodies were completely unable to bind natural Gb3Cer both inserted into cells and in artificial membrane, whereas strong interaction took place with synthetic analogue differing by the presence of a spacer between trisaccharide and lipid part.
28708979	10	57	theme	membrane-bound	1628:1641	arg1	glycans					1643:1649	membrane-bound glycans	1628:1649	membrane-bound glycans	1628:1649	MD simulations revealed: i) although membrane-bound glycans do not form stable long-living aggregates, their transient packing is more compact in natural Gb3 as compared with the synthetic analog, ii) similar conformation of Pk glycan in composition of the glycolipids, iii) no effect on the mentioned above results when cholesterol was inserted into membrane, and iv) better accessibility of the synthetic version for interaction with proteins.
28708979	2	58	theme	natural	214:220	arg1	antibodies					222:231	natural antibodies	214:231	natural antibodies	214:231	BACKGROUND Human blood contains a big variety of natural antibodies, circulating throughout life at constant concentration.
28708979	10	59	dep	effect	1869:1874	arg1	iii					1861:1863	iii	1861:1863	iii	1861:1863	MD simulations revealed: i) although membrane-bound glycans do not form stable long-living aggregates, their transient packing is more compact in natural Gb3 as compared with the synthetic analog, ii) similar conformation of Pk glycan in composition of the glycolipids, iii) no effect on the mentioned above results when cholesterol was inserted into membrane, and iv) better accessibility of the synthetic version for interaction with proteins.
28708979	4	60	theme	immunological	658:670	arg1	reaction					672:679	any immunological reaction	654:679	any immunological reaction	654:679	Intriguingly, the same trisaccharide is a key fragment of glycosphingolipid globotriaosylceramide (Gb3Cer) - normal component of erythrocyte and endothelial cell membrane, i.e. the antibodies and their cognate antigen coexist without any immunological reaction.
28708979	10	61	theme	better	1960:1965	arg1	accessibility					1967:1979	better accessibility	1960:1979	iv) better accessibility of the synthetic version for interaction with proteins	1956:2034	MD simulations revealed: i) although membrane-bound glycans do not form stable long-living aggregates, their transient packing is more compact in natural Gb3 as compared with the synthetic analog, ii) similar conformation of Pk glycan in composition of the glycolipids, iii) no effect on the mentioned above results when cholesterol was inserted into membrane, and iv) better accessibility of the synthetic version for interaction with proteins.
28708979	12	62	theme	group	2323:2327	arg1	system					2331:2336	the blood group P system	2313:2336	the blood group P system	2313:2336	We can conclude that the antibodies are not related to the blood group P system.
28708979	11	63	theme	anti-Pk	2149:2155	arg1	antibodies					2157:2166	natural anti-Pk antibodies	2141:2166	natural anti-Pk antibodies	2141:2166	CONCLUSIONS Both immunochemical and molecular dynamics data argue that the reason of the "tolerance" of natural anti-Pk antibodies towards cell-bound Gb3Cer is the spatial inaccessibility of Pk glycotope for interaction.
28708979	10	64	theme	Pk	1816:1817	arg1	glycan					1819:1824	Pk glycan	1816:1824	Pk glycan in composition of the glycolipids	1816:1858	MD simulations revealed: i) although membrane-bound glycans do not form stable long-living aggregates, their transient packing is more compact in natural Gb3 as compared with the synthetic analog, ii) similar conformation of Pk glycan in composition of the glycolipids, iii) no effect on the mentioned above results when cholesterol was inserted into membrane, and iv) better accessibility of the synthetic version for interaction with proteins.
28708979	8	65	theme	MD	1301:1302	arg1	simulations					1304:1314	MD simulations	1301:1314	MD simulations	1301:1314	Conformations and clustering of glycolipids immersed into a lipid bilayer were studied using MD simulations.
28708979	2	66	theme	BACKGROUND	165:174	arg1	blood					182:186	BACKGROUND Human blood	165:186	BACKGROUND Human blood	165:186	BACKGROUND Human blood contains a big variety of natural antibodies, circulating throughout life at constant concentration.
28708979	10	67	theme	glycolipids	1848:1858	arg1	composition					1829:1839	composition	1829:1839	composition of the glycolipids	1829:1858	MD simulations revealed: i) although membrane-bound glycans do not form stable long-living aggregates, their transient packing is more compact in natural Gb3 as compared with the synthetic analog, ii) similar conformation of Pk glycan in composition of the glycolipids, iii) no effect on the mentioned above results when cholesterol was inserted into membrane, and iv) better accessibility of the synthetic version for interaction with proteins.
28708979	10	68	from	conformation	1800:1811	arg1	results					1899:1905	the mentioned above results	1879:1905	the mentioned above results when cholesterol was inserted into membrane	1879:1949	MD simulations revealed: i) although membrane-bound glycans do not form stable long-living aggregates, their transient packing is more compact in natural Gb3 as compared with the synthetic analog, ii) similar conformation of Pk glycan in composition of the glycolipids, iii) no effect on the mentioned above results when cholesterol was inserted into membrane, and iv) better accessibility of the synthetic version for interaction with proteins.
28708979	10	68	from	conformation	1800:1811	arg1	composition					1829:1839	composition	1829:1839	composition of the glycolipids	1829:1858	MD simulations revealed: i) although membrane-bound glycans do not form stable long-living aggregates, their transient packing is more compact in natural Gb3 as compared with the synthetic analog, ii) similar conformation of Pk glycan in composition of the glycolipids, iii) no effect on the mentioned above results when cholesterol was inserted into membrane, and iv) better accessibility of the synthetic version for interaction with proteins.
28708979	10	69	theme	long-living	1670:1680	arg1	aggregates					1682:1691	stable long-living aggregates	1663:1691	stable long-living aggregates	1663:1691	MD simulations revealed: i) although membrane-bound glycans do not form stable long-living aggregates, their transient packing is more compact in natural Gb3 as compared with the synthetic analog, ii) similar conformation of Pk glycan in composition of the glycolipids, iii) no effect on the mentioned above results when cholesterol was inserted into membrane, and iv) better accessibility of the synthetic version for interaction with proteins.
28708979	6	70	theme	molecular	824:832	arg1	MD					844:845	MD	844:845	MD	844:845	MATERIALS AND METHODS We used a combination of immunochemical and molecular dynamics (MD) experiments.
28708979	6	70	theme	molecular	824:832	arg1	dynamics					834:841	molecular dynamics	824:841	molecular dynamics (MD)	824:846	MATERIALS AND METHODS We used a combination of immunochemical and molecular dynamics (MD) experiments.
28708979	7	71	theme	affinity	892:899	arg1	media					901:905	affinity media	892:905	affinity media with Pk trisaccharide	892:927	Antibodies were isolated using affinity media with Pk trisaccharide, their epitope specificity was characterized using ELISA (enzyme-linked immunosorbent assay) with a set of synthetic glycans related to Pk synthetic glycans and FACS (Fluorescence-Activated Cell Sorting) analysis of cells with inserted natural Gb3Cer and its synthetic analogue.
28708979	3	72	theme	natural	315:321	arg1	antibodies					323:332	natural antibodies	315:332	natural antibodies capable of binding to trisaccharide Galα1-4Galβ1-4Glc (Pk)	315:391	Previously, we have found natural antibodies capable of binding to trisaccharide Galα1-4Galβ1-4Glc (Pk) practically in all humans.
28708979	4	73	theme	glycosphingolipid	478:494	arg1	Gb3Cer					519:524	Gb3Cer	519:524	Gb3Cer	519:524	Intriguingly, the same trisaccharide is a key fragment of glycosphingolipid globotriaosylceramide (Gb3Cer) - normal component of erythrocyte and endothelial cell membrane, i.e. the antibodies and their cognate antigen coexist without any immunological reaction.
28708979	4	73	theme	glycosphingolipid	478:494	arg1	globotriaosylceramide					496:516	glycosphingolipid globotriaosylceramide	478:516	glycosphingolipid globotriaosylceramide (Gb3Cer)	478:525	Intriguingly, the same trisaccharide is a key fragment of glycosphingolipid globotriaosylceramide (Gb3Cer) - normal component of erythrocyte and endothelial cell membrane, i.e. the antibodies and their cognate antigen coexist without any immunological reaction.
28708979	10	74	theme	synthetic	1988:1996	arg1	version					1998:2004	the synthetic version	1984:2004	the synthetic version for interaction with proteins	1984:2034	MD simulations revealed: i) although membrane-bound glycans do not form stable long-living aggregates, their transient packing is more compact in natural Gb3 as compared with the synthetic analog, ii) similar conformation of Pk glycan in composition of the glycolipids, iii) no effect on the mentioned above results when cholesterol was inserted into membrane, and iv) better accessibility of the synthetic version for interaction with proteins.
28708979	7	75	theme	Cell	1119:1122	arg1	Sorting					1124:1130	Fluorescence-Activated Cell Sorting	1096:1130	Fluorescence-Activated Cell Sorting	1096:1130	Antibodies were isolated using affinity media with Pk trisaccharide, their epitope specificity was characterized using ELISA (enzyme-linked immunosorbent assay) with a set of synthetic glycans related to Pk synthetic glycans and FACS (Fluorescence-Activated Cell Sorting) analysis of cells with inserted natural Gb3Cer and its synthetic analogue.
28708979	7	75	theme	Cell	1119:1122	arg1	FACS					1090:1093	FACS	1090:1093	FACS (Fluorescence-Activated Cell Sorting) analysis of cells with inserted natural Gb3Cer and its synthetic analogue	1090:1205	Antibodies were isolated using affinity media with Pk trisaccharide, their epitope specificity was characterized using ELISA (enzyme-linked immunosorbent assay) with a set of synthetic glycans related to Pk synthetic glycans and FACS (Fluorescence-Activated Cell Sorting) analysis of cells with inserted natural Gb3Cer and its synthetic analogue.
28708979	2	76	theme	constant	265:272	arg1	concentration					274:286	constant concentration	265:286	constant concentration	265:286	BACKGROUND Human blood contains a big variety of natural antibodies, circulating throughout life at constant concentration.
28708979	0	77	from	trisaccharide	73:85	arg1	membrane					102:109	erythrocyte membrane	90:109	erythrocyte membrane	90:109	Why human anti-Galα1-4Galβ1-4Glc natural antibodies do not recognize the trisaccharide on erythrocyte membrane?
28708979	10	78	theme	transient	1700:1708	arg1	packing					1710:1716	their transient packing	1694:1716	their transient packing	1694:1716	MD simulations revealed: i) although membrane-bound glycans do not form stable long-living aggregates, their transient packing is more compact in natural Gb3 as compared with the synthetic analog, ii) similar conformation of Pk glycan in composition of the glycolipids, iii) no effect on the mentioned above results when cholesterol was inserted into membrane, and iv) better accessibility of the synthetic version for interaction with proteins.
28708979	5	79	theme	anti-Pk	720:726	arg1	antibodies					728:737	human anti-Pk antibodies	714:737	human anti-Pk antibodies	714:737	AIM To explain the inertness of human anti-Pk antibodies towards own cells.
28708979	9	80	theme	specific	1334:1341	arg1	antibodies					1343:1352	specific antibodies	1334:1352	specific antibodies	1334:1352	RESULTS Isolated specific antibodies were completely unable to bind natural Gb3Cer both inserted into cells and in artificial membrane, whereas strong interaction took place with synthetic analogue differing by the presence of a spacer between trisaccharide and lipid part.
28708979	10	81	theme	mentioned	1883:1891	arg1	results					1899:1905	the mentioned above results	1879:1905	the mentioned above results when cholesterol was inserted into membrane	1879:1949	MD simulations revealed: i) although membrane-bound glycans do not form stable long-living aggregates, their transient packing is more compact in natural Gb3 as compared with the synthetic analog, ii) similar conformation of Pk glycan in composition of the glycolipids, iii) no effect on the mentioned above results when cholesterol was inserted into membrane, and iv) better accessibility of the synthetic version for interaction with proteins.
28708979	7	82	theme	Pk	1065:1066	arg1	glycans					1078:1084	Pk synthetic glycans	1065:1084	Pk synthetic glycans	1065:1084	Antibodies were isolated using affinity media with Pk trisaccharide, their epitope specificity was characterized using ELISA (enzyme-linked immunosorbent assay) with a set of synthetic glycans related to Pk synthetic glycans and FACS (Fluorescence-Activated Cell Sorting) analysis of cells with inserted natural Gb3Cer and its synthetic analogue.
28708979	6	83	used	used	783:786	arg2	METHODS					772:778	METHODS	772:778	METHODS	772:778	MATERIALS AND METHODS We used a combination of immunochemical and molecular dynamics (MD) experiments.
28708979	6	83	used	used	783:786	arg2	MATERIALS					758:766	MATERIALS	758:766	MATERIALS	758:766	MATERIALS AND METHODS We used a combination of immunochemical and molecular dynamics (MD) experiments.
28708979	3	84	theme	capable	334:340	arg1	antibodies					323:332	natural antibodies	315:332	natural antibodies capable of binding to trisaccharide Galα1-4Galβ1-4Glc (Pk)	315:391	Previously, we have found natural antibodies capable of binding to trisaccharide Galα1-4Galβ1-4Glc (Pk) practically in all humans.
28708979	7	85	link	enzyme-linked	987:999	arg1	assay					1015:1019	enzyme-linked immunosorbent assay	987:1019	enzyme-linked immunosorbent assay	987:1019	Antibodies were isolated using affinity media with Pk trisaccharide, their epitope specificity was characterized using ELISA (enzyme-linked immunosorbent assay) with a set of synthetic glycans related to Pk synthetic glycans and FACS (Fluorescence-Activated Cell Sorting) analysis of cells with inserted natural Gb3Cer and its synthetic analogue.
28708979	7	85	link	enzyme-linked	987:999	arg1	ELISA					980:984	ELISA	980:984	ELISA (enzyme-linked immunosorbent assay) with a set of synthetic glycans related to Pk synthetic glycans	980:1084	Antibodies were isolated using affinity media with Pk trisaccharide, their epitope specificity was characterized using ELISA (enzyme-linked immunosorbent assay) with a set of synthetic glycans related to Pk synthetic glycans and FACS (Fluorescence-Activated Cell Sorting) analysis of cells with inserted natural Gb3Cer and its synthetic analogue.
28708979	7	86	theme	related	1054:1060	arg1	glycans					1046:1052	synthetic glycans	1036:1052	synthetic glycans related to Pk synthetic glycans	1036:1084	Antibodies were isolated using affinity media with Pk trisaccharide, their epitope specificity was characterized using ELISA (enzyme-linked immunosorbent assay) with a set of synthetic glycans related to Pk synthetic glycans and FACS (Fluorescence-Activated Cell Sorting) analysis of cells with inserted natural Gb3Cer and its synthetic analogue.
28708979	10	87	from	glycan	1819:1824	arg1	composition					1829:1839	composition	1829:1839	composition of the glycolipids	1829:1858	MD simulations revealed: i) although membrane-bound glycans do not form stable long-living aggregates, their transient packing is more compact in natural Gb3 as compared with the synthetic analog, ii) similar conformation of Pk glycan in composition of the glycolipids, iii) no effect on the mentioned above results when cholesterol was inserted into membrane, and iv) better accessibility of the synthetic version for interaction with proteins.
28708979	12	88	theme	P	2329:2329	arg1	system					2331:2336	the blood group P system	2313:2336	the blood group P system	2313:2336	We can conclude that the antibodies are not related to the blood group P system.
28708979	0	89	theme	erythrocyte	90:100	arg1	membrane					102:109	erythrocyte membrane	90:109	erythrocyte membrane	90:109	Why human anti-Galα1-4Galβ1-4Glc natural antibodies do not recognize the trisaccharide on erythrocyte membrane?
28708979	7	90	theme	synthetic	1036:1044	arg1	glycans					1046:1052	synthetic glycans	1036:1052	synthetic glycans related to Pk synthetic glycans	1036:1084	Antibodies were isolated using affinity media with Pk trisaccharide, their epitope specificity was characterized using ELISA (enzyme-linked immunosorbent assay) with a set of synthetic glycans related to Pk synthetic glycans and FACS (Fluorescence-Activated Cell Sorting) analysis of cells with inserted natural Gb3Cer and its synthetic analogue.
28708979	10	91	from	composition	1829:1839	arg1	accessibility					1967:1979	better accessibility	1960:1979	iv) better accessibility of the synthetic version for interaction with proteins	1956:2034	MD simulations revealed: i) although membrane-bound glycans do not form stable long-living aggregates, their transient packing is more compact in natural Gb3 as compared with the synthetic analog, ii) similar conformation of Pk glycan in composition of the glycolipids, iii) no effect on the mentioned above results when cholesterol was inserted into membrane, and iv) better accessibility of the synthetic version for interaction with proteins.
28708979	10	91	from	composition	1829:1839	arg1	effect					1869:1874	no effect	1866:1874	iii) no effect on the mentioned above results when cholesterol was inserted into membrane	1861:1949	MD simulations revealed: i) although membrane-bound glycans do not form stable long-living aggregates, their transient packing is more compact in natural Gb3 as compared with the synthetic analog, ii) similar conformation of Pk glycan in composition of the glycolipids, iii) no effect on the mentioned above results when cholesterol was inserted into membrane, and iv) better accessibility of the synthetic version for interaction with proteins.
28708979	10	91	from	composition	1829:1839	arg1	conformation					1800:1811	ii) similar conformation	1788:1811	ii) similar conformation of Pk glycan in composition of the glycolipids	1788:1858	MD simulations revealed: i) although membrane-bound glycans do not form stable long-living aggregates, their transient packing is more compact in natural Gb3 as compared with the synthetic analog, ii) similar conformation of Pk glycan in composition of the glycolipids, iii) no effect on the mentioned above results when cholesterol was inserted into membrane, and iv) better accessibility of the synthetic version for interaction with proteins.
28708979	3	92	theme	trisaccharide	356:368	arg1	Galα1-4Galβ1-4Glc					370:386	trisaccharide Galα1-4Galβ1-4Glc	356:386	trisaccharide Galα1-4Galβ1-4Glc (Pk)	356:391	Previously, we have found natural antibodies capable of binding to trisaccharide Galα1-4Galβ1-4Glc (Pk) practically in all humans.
28708979	3	92	theme	trisaccharide	356:368	arg1	Pk					389:390	Pk	389:390	Pk	389:390	Previously, we have found natural antibodies capable of binding to trisaccharide Galα1-4Galβ1-4Glc (Pk) practically in all humans.
28708979	4	93	theme	normal	529:534	arg1	component					536:544	normal component	529:544	normal component of erythrocyte and endothelial cell membrane, i.e. the antibodies and their cognate antigen coexist without any immunological reaction	529:679	Intriguingly, the same trisaccharide is a key fragment of glycosphingolipid globotriaosylceramide (Gb3Cer) - normal component of erythrocyte and endothelial cell membrane, i.e. the antibodies and their cognate antigen coexist without any immunological reaction.
28708979	0	94	theme	human	4:8	arg1	antibodies					41:50	human anti-Galα1-4Galβ1-4Glc natural antibodies	4:50	human anti-Galα1-4Galβ1-4Glc natural antibodies	4:50	Why human anti-Galα1-4Galβ1-4Glc natural antibodies do not recognize the trisaccharide on erythrocyte membrane?
28708979	11	95	theme	tolerance	2127:2135	arg1	"					2136:2136	the "tolerance"	2122:2136	the "tolerance" of natural anti-Pk antibodies towards cell-bound Gb3Cer	2122:2192	CONCLUSIONS Both immunochemical and molecular dynamics data argue that the reason of the "tolerance" of natural anti-Pk antibodies towards cell-bound Gb3Cer is the spatial inaccessibility of Pk glycotope for interaction.
28708979	10	96	dep	form	1658:1661	arg1	i					1616:1616	i	1616:1616	i	1616:1616	MD simulations revealed: i) although membrane-bound glycans do not form stable long-living aggregates, their transient packing is more compact in natural Gb3 as compared with the synthetic analog, ii) similar conformation of Pk glycan in composition of the glycolipids, iii) no effect on the mentioned above results when cholesterol was inserted into membrane, and iv) better accessibility of the synthetic version for interaction with proteins.
28708979	10	97	dep	accessibility	1967:1979	arg1	iv					1956:1957	iv	1956:1957	iv	1956:1957	MD simulations revealed: i) although membrane-bound glycans do not form stable long-living aggregates, their transient packing is more compact in natural Gb3 as compared with the synthetic analog, ii) similar conformation of Pk glycan in composition of the glycolipids, iii) no effect on the mentioned above results when cholesterol was inserted into membrane, and iv) better accessibility of the synthetic version for interaction with proteins.
28708979	0	98	theme	natural	33:39	arg1	antibodies					41:50	human anti-Galα1-4Galβ1-4Glc natural antibodies	4:50	human anti-Galα1-4Galβ1-4Glc natural antibodies	4:50	Why human anti-Galα1-4Galβ1-4Glc natural antibodies do not recognize the trisaccharide on erythrocyte membrane?
28708979	7	99	theme	enzyme-linked	987:999	arg1	assay					1015:1019	enzyme-linked immunosorbent assay	987:1019	enzyme-linked immunosorbent assay	987:1019	Antibodies were isolated using affinity media with Pk trisaccharide, their epitope specificity was characterized using ELISA (enzyme-linked immunosorbent assay) with a set of synthetic glycans related to Pk synthetic glycans and FACS (Fluorescence-Activated Cell Sorting) analysis of cells with inserted natural Gb3Cer and its synthetic analogue.
28708979	7	99	theme	enzyme-linked	987:999	arg1	ELISA					980:984	ELISA	980:984	ELISA (enzyme-linked immunosorbent assay) with a set of synthetic glycans related to Pk synthetic glycans	980:1084	Antibodies were isolated using affinity media with Pk trisaccharide, their epitope specificity was characterized using ELISA (enzyme-linked immunosorbent assay) with a set of synthetic glycans related to Pk synthetic glycans and FACS (Fluorescence-Activated Cell Sorting) analysis of cells with inserted natural Gb3Cer and its synthetic analogue.
28708979	1	100	theme	Molecular	112:120	arg1	dynamics					122:129	Molecular dynamics	112:129	Molecular dynamics	112:129	Molecular dynamics and immunochemical investigation.
28708979	10	101	from	effect	1869:1874	arg1	results					1899:1905	the mentioned above results	1879:1905	the mentioned above results when cholesterol was inserted into membrane	1879:1949	MD simulations revealed: i) although membrane-bound glycans do not form stable long-living aggregates, their transient packing is more compact in natural Gb3 as compared with the synthetic analog, ii) similar conformation of Pk glycan in composition of the glycolipids, iii) no effect on the mentioned above results when cholesterol was inserted into membrane, and iv) better accessibility of the synthetic version for interaction with proteins.
28708979	10	101	from	effect	1869:1874	arg1	composition					1829:1839	composition	1829:1839	composition of the glycolipids	1829:1858	MD simulations revealed: i) although membrane-bound glycans do not form stable long-living aggregates, their transient packing is more compact in natural Gb3 as compared with the synthetic analog, ii) similar conformation of Pk glycan in composition of the glycolipids, iii) no effect on the mentioned above results when cholesterol was inserted into membrane, and iv) better accessibility of the synthetic version for interaction with proteins.
28708979	4	102	theme	cell	577:580	arg1	membrane					582:589	erythrocyte and endothelial cell membrane	549:589	membrane	582:589	Intriguingly, the same trisaccharide is a key fragment of glycosphingolipid globotriaosylceramide (Gb3Cer) - normal component of erythrocyte and endothelial cell membrane, i.e. the antibodies and their cognate antigen coexist without any immunological reaction.
28708979	2	103	theme	big	199:201	arg1	variety					203:209	a big variety	197:209	a big variety of natural antibodies	197:231	BACKGROUND Human blood contains a big variety of natural antibodies, circulating throughout life at constant concentration.
28708979	2	103	theme	big	199:201	arg1	antibodies					222:231	natural antibodies	214:231	natural antibodies	214:231	BACKGROUND Human blood contains a big variety of natural antibodies, circulating throughout life at constant concentration.
28708979	7	104	theme	synthetic	1188:1196	arg1	analogue					1198:1205	its synthetic analogue	1184:1205	its synthetic analogue	1184:1205	Antibodies were isolated using affinity media with Pk trisaccharide, their epitope specificity was characterized using ELISA (enzyme-linked immunosorbent assay) with a set of synthetic glycans related to Pk synthetic glycans and FACS (Fluorescence-Activated Cell Sorting) analysis of cells with inserted natural Gb3Cer and its synthetic analogue.
28708979	12	105	theme	blood	2317:2321	arg1	system					2331:2336	the blood group P system	2313:2336	the blood group P system	2313:2336	We can conclude that the antibodies are not related to the blood group P system.
28708979	10	106	theme	MD	1591:1592	arg1	simulations					1594:1604	MD simulations	1591:1604	MD simulations	1591:1604	MD simulations revealed: i) although membrane-bound glycans do not form stable long-living aggregates, their transient packing is more compact in natural Gb3 as compared with the synthetic analog, ii) similar conformation of Pk glycan in composition of the glycolipids, iii) no effect on the mentioned above results when cholesterol was inserted into membrane, and iv) better accessibility of the synthetic version for interaction with proteins.
28708979	2	107	theme	antibodies	222:231	arg1	variety					203:209	a big variety	197:209	a big variety of natural antibodies	197:231	BACKGROUND Human blood contains a big variety of natural antibodies, circulating throughout life at constant concentration.
28708979	2	107	theme	antibodies	222:231	arg1	antibodies					222:231	natural antibodies	214:231	natural antibodies	214:231	BACKGROUND Human blood contains a big variety of natural antibodies, circulating throughout life at constant concentration.
28708979	10	108	from	Gb3	1745:1747	arg1	compact					1726:1732	compact	1726:1732	compact	1726:1732	MD simulations revealed: i) although membrane-bound glycans do not form stable long-living aggregates, their transient packing is more compact in natural Gb3 as compared with the synthetic analog, ii) similar conformation of Pk glycan in composition of the glycolipids, iii) no effect on the mentioned above results when cholesterol was inserted into membrane, and iv) better accessibility of the synthetic version for interaction with proteins.
28708979	10	109	theme	similar	1792:1798	arg1	conformation					1800:1811	ii) similar conformation	1788:1811	ii) similar conformation of Pk glycan in composition of the glycolipids	1788:1858	MD simulations revealed: i) although membrane-bound glycans do not form stable long-living aggregates, their transient packing is more compact in natural Gb3 as compared with the synthetic analog, ii) similar conformation of Pk glycan in composition of the glycolipids, iii) no effect on the mentioned above results when cholesterol was inserted into membrane, and iv) better accessibility of the synthetic version for interaction with proteins.
28708979	7	110	theme	epitope	936:942	arg1	specificity					944:954	their epitope specificity	930:954	their epitope specificity	930:954	Antibodies were isolated using affinity media with Pk trisaccharide, their epitope specificity was characterized using ELISA (enzyme-linked immunosorbent assay) with a set of synthetic glycans related to Pk synthetic glycans and FACS (Fluorescence-Activated Cell Sorting) analysis of cells with inserted natural Gb3Cer and its synthetic analogue.
25136131	4	0	theme	aromatic	599:606	arg1	aldehydes					608:616	aromatic aldehydes	599:616	aromatic aldehydes derived from lignin and hemicellulose	599:654	To replace imidazolium-based ILs with those derived from renewable sources, we synthesized a series of tertiary amine-based ILs from aromatic aldehydes derived from lignin and hemicellulose, the major by-products of lignocellulosic biofuel production.
25136131	11	1	theme	closed-loop	1832:1842	arg1	process					1845:1851	a "closed-loop" process	1829:1851	a "closed-loop" process	1829:1851	Our concept of deriving ILs from lignocellulosic biomass shows significant potential for the realization of a "closed-loop" process for future lignocellulosic biorefineries and has far-reaching economic impacts for other IL-based process technology currently using ILs synthesized from petroleum sources.
25136131	6	2	theme	pretreated	878:887	arg1	switchgrass					889:899	pretreated switchgrass	878:899	pretreated switchgrass	878:899	Enzymatic hydrolysis of pretreated switchgrass allowed for direct comparison of sugar yields and lignin removal between biomass-derived ILs and 1-ethyl-3-methylimidazolium acetate.
25136131	4	3	attach	derived	510:516	arg1	sources					533:539	renewable sources	523:539	renewable sources	523:539	To replace imidazolium-based ILs with those derived from renewable sources, we synthesized a series of tertiary amine-based ILs from aromatic aldehydes derived from lignin and hemicellulose, the major by-products of lignocellulosic biofuel production.
25136131	4	3	attach	derived	510:516	arg2	those					504:508	those	504:508	those	504:508	To replace imidazolium-based ILs with those derived from renewable sources, we synthesized a series of tertiary amine-based ILs from aromatic aldehydes derived from lignin and hemicellulose, the major by-products of lignocellulosic biofuel production.
25136131	1	4	theme	applications	224:235	arg1	applications					224:235	renewable energy applications	207:235	renewable energy applications	207:235	Ionic liquids (ILs), solvents composed entirely of paired ions, have been used in a variety of process chemistry and renewable energy applications.
25136131	1	4	theme	applications	224:235	arg1	variety					174:180	a variety	172:180	a variety of process chemistry and renewable energy applications	172:235	Ionic liquids (ILs), solvents composed entirely of paired ions, have been used in a variety of process chemistry and renewable energy applications.
25136131	1	4	theme	applications	224:235	arg1	chemistry					193:201	process chemistry	185:201	process chemistry	185:201	Ionic liquids (ILs), solvents composed entirely of paired ions, have been used in a variety of process chemistry and renewable energy applications.
25136131	6	5	theme	biomass-derived	974:988	arg1	ILs					990:992	biomass-derived ILs	974:992	biomass-derived ILs	974:992	Enzymatic hydrolysis of pretreated switchgrass allowed for direct comparison of sugar yields and lignin removal between biomass-derived ILs and 1-ethyl-3-methylimidazolium acetate.
25136131	7	6	theme	xylose	1225:1230	arg1	yields					1232:1237	90-95% glucose and 70-75% xylose yields	1199:1237	90-95% glucose and 70-75% xylose yields	1199:1237	Although the rate of cellulose hydrolysis for switchgrass pretreated with biomass-derived ILs was slightly slower than that of 1-ethyl-3-methylimidazolium acetate, 90-95% glucose and 70-75% xylose yields were obtained for these samples after 72-h incubation.
25136131	6	7	theme	removal	958:964	arg1	comparison					920:929	direct comparison	913:929	direct comparison of sugar yields and lignin removal between biomass-derived ILs and 1-ethyl-3-methylimidazolium acetate	913:1032	Enzymatic hydrolysis of pretreated switchgrass allowed for direct comparison of sugar yields and lignin removal between biomass-derived ILs and 1-ethyl-3-methylimidazolium acetate.
25136131	4	8	theme	lignocellulosic	682:696	arg1	production					706:715	lignocellulosic biofuel production	682:715	lignocellulosic biofuel production	682:715	To replace imidazolium-based ILs with those derived from renewable sources, we synthesized a series of tertiary amine-based ILs from aromatic aldehydes derived from lignin and hemicellulose, the major by-products of lignocellulosic biofuel production.
25136131	11	9	theme	future	1857:1862	arg1	biorefineries					1880:1892	future lignocellulosic biorefineries	1857:1892	future lignocellulosic biorefineries	1857:1892	Our concept of deriving ILs from lignocellulosic biomass shows significant potential for the realization of a "closed-loop" process for future lignocellulosic biorefineries and has far-reaching economic impacts for other IL-based process technology currently using ILs synthesized from petroleum sources.
25136131	7	10	theme	70-75	1218:1222	arg1	%					1223:1223	%	1223:1223	%	1223:1223	Although the rate of cellulose hydrolysis for switchgrass pretreated with biomass-derived ILs was slightly slower than that of 1-ethyl-3-methylimidazolium acetate, 90-95% glucose and 70-75% xylose yields were obtained for these samples after 72-h incubation.
25136131	10	11	theme	cellulose	1587:1595	arg1	changes					1608:1614	different cellulose structural changes	1577:1614	different cellulose structural changes	1577:1614	These studies showed different cellulose structural changes and differences in hemicellulose epitopes between switchgrass pretreatments with the aforementioned ILs.
25136131	8	12	theme	obtained	1375:1382	arg1	data					1407:1410	experimentally obtained compositional analysis data	1360:1410	experimentally obtained compositional analysis data	1360:1410	Molecular modeling was used to compare IL solvent parameters with experimentally obtained compositional analysis data.
25136131	7	13	theme	glucose	1206:1212	arg1	yields					1232:1237	90-95% glucose and 70-75% xylose yields	1199:1237	90-95% glucose and 70-75% xylose yields	1199:1237	Although the rate of cellulose hydrolysis for switchgrass pretreated with biomass-derived ILs was slightly slower than that of 1-ethyl-3-methylimidazolium acetate, 90-95% glucose and 70-75% xylose yields were obtained for these samples after 72-h incubation.
25136131	8	14	theme	analysis	1398:1405	arg1	data					1407:1410	experimentally obtained compositional analysis data	1360:1410	experimentally obtained compositional analysis data	1360:1410	Molecular modeling was used to compare IL solvent parameters with experimentally obtained compositional analysis data.
25136131	7	15	theme	90-95	1199:1203	arg1	%					1204:1204	%	1204:1204	%	1204:1204	Although the rate of cellulose hydrolysis for switchgrass pretreated with biomass-derived ILs was slightly slower than that of 1-ethyl-3-methylimidazolium acetate, 90-95% glucose and 70-75% xylose yields were obtained for these samples after 72-h incubation.
25136131	7	16	link	biomass-derived	1109:1123	arg1	ILs					1125:1127	biomass-derived ILs	1109:1127	biomass-derived ILs	1109:1127	Although the rate of cellulose hydrolysis for switchgrass pretreated with biomass-derived ILs was slightly slower than that of 1-ethyl-3-methylimidazolium acetate, 90-95% glucose and 70-75% xylose yields were obtained for these samples after 72-h incubation.
25136131	8	17	theme	solvent	1336:1342	arg1	parameters					1344:1353	IL solvent parameters	1333:1353	IL solvent parameters	1333:1353	Molecular modeling was used to compare IL solvent parameters with experimentally obtained compositional analysis data.
25136131	5	18	theme	Compositional	718:730	arg1	analysis					732:739	Compositional analysis	718:739	Compositional analysis of switchgrass pretreated with ILs derived from vanillin, p-anisaldehyde, and furfural	718:826	Compositional analysis of switchgrass pretreated with ILs derived from vanillin, p-anisaldehyde, and furfural confirmed their efficacy.
25136131	7	19	theme	biomass-derived	1109:1123	arg1	ILs					1125:1127	biomass-derived ILs	1109:1127	biomass-derived ILs	1109:1127	Although the rate of cellulose hydrolysis for switchgrass pretreated with biomass-derived ILs was slightly slower than that of 1-ethyl-3-methylimidazolium acetate, 90-95% glucose and 70-75% xylose yields were obtained for these samples after 72-h incubation.
25136131	10	20	with	pretreatments	1678:1690	arg1	ILs					1716:1718	the aforementioned ILs	1697:1718	the aforementioned ILs	1697:1718	These studies showed different cellulose structural changes and differences in hemicellulose epitopes between switchgrass pretreatments with the aforementioned ILs.
25136131	1	21	used	used	164:167	arg2	ILs					105:107	ILs	105:107	ILs	105:107	Ionic liquids (ILs), solvents composed entirely of paired ions, have been used in a variety of process chemistry and renewable energy applications.
25136131	1	21	used	used	164:167	arg2	liquids					96:102	Ionic liquids	90:102	Ionic liquids (ILs)	90:108	Ionic liquids (ILs), solvents composed entirely of paired ions, have been used in a variety of process chemistry and renewable energy applications.
25136131	1	21	used	used	164:167	arg2	solvents					111:118	solvents	111:118	solvents composed entirely of paired ions	111:151	Ionic liquids (ILs), solvents composed entirely of paired ions, have been used in a variety of process chemistry and renewable energy applications.
25136131	9	22	theme	Effective	1413:1421	arg1	pretreatment					1423:1434	Effective pretreatment	1413:1434	Effective pretreatment of lignocellulose	1413:1452	Effective pretreatment of lignocellulose was further investigated by powder X-ray diffraction and glycome profiling of switchgrass cell walls.
25136131	4	23	attach	derived	618:624	arg1	lignin					631:636	lignin	631:636	lignin	631:636	To replace imidazolium-based ILs with those derived from renewable sources, we synthesized a series of tertiary amine-based ILs from aromatic aldehydes derived from lignin and hemicellulose, the major by-products of lignocellulosic biofuel production.
25136131	4	23	attach	derived	618:624	arg1	hemicellulose					642:654	hemicellulose	642:654	hemicellulose	642:654	To replace imidazolium-based ILs with those derived from renewable sources, we synthesized a series of tertiary amine-based ILs from aromatic aldehydes derived from lignin and hemicellulose, the major by-products of lignocellulosic biofuel production.
25136131	4	23	attach	derived	618:624	arg2	aldehydes					608:616	aromatic aldehydes	599:616	aromatic aldehydes derived from lignin and hemicellulose	599:654	To replace imidazolium-based ILs with those derived from renewable sources, we synthesized a series of tertiary amine-based ILs from aromatic aldehydes derived from lignin and hemicellulose, the major by-products of lignocellulosic biofuel production.
25136131	11	24	theme	petroleum	2007:2015	arg1	sources					2017:2023	petroleum sources	2007:2023	petroleum sources	2007:2023	Our concept of deriving ILs from lignocellulosic biomass shows significant potential for the realization of a "closed-loop" process for future lignocellulosic biorefineries and has far-reaching economic impacts for other IL-based process technology currently using ILs synthesized from petroleum sources.
25136131	7	25	theme	cellulose	1056:1064	arg1	hydrolysis					1066:1075	cellulose hydrolysis	1056:1075	cellulose hydrolysis	1056:1075	Although the rate of cellulose hydrolysis for switchgrass pretreated with biomass-derived ILs was slightly slower than that of 1-ethyl-3-methylimidazolium acetate, 90-95% glucose and 70-75% xylose yields were obtained for these samples after 72-h incubation.
25136131	4	26	from	aldehydes	608:616	arg1	series					559:564	a series	557:564	a series of tertiary amine-based ILs from aromatic aldehydes derived from lignin and hemicellulose	557:654	To replace imidazolium-based ILs with those derived from renewable sources, we synthesized a series of tertiary amine-based ILs from aromatic aldehydes derived from lignin and hemicellulose, the major by-products of lignocellulosic biofuel production.
25136131	4	26	from	aldehydes	608:616	arg1	by-products					667:677	the major by-products	657:677	the major by-products of lignocellulosic biofuel production	657:715	To replace imidazolium-based ILs with those derived from renewable sources, we synthesized a series of tertiary amine-based ILs from aromatic aldehydes derived from lignin and hemicellulose, the major by-products of lignocellulosic biofuel production.
25136131	4	26	from	aldehydes	608:616	arg1	ILs					590:592	tertiary amine-based ILs	569:592	tertiary amine-based ILs from aromatic aldehydes derived from lignin and hemicellulose	569:654	To replace imidazolium-based ILs with those derived from renewable sources, we synthesized a series of tertiary amine-based ILs from aromatic aldehydes derived from lignin and hemicellulose, the major by-products of lignocellulosic biofuel production.
25136131	3	27	theme	industrial	443:452	arg1	deployment					454:463	their large-scale industrial deployment	425:463	their large-scale industrial deployment	425:463	Although efficient, imidazolium cations are expensive and thus limited in their large-scale industrial deployment.
25136131	10	28	theme	hemicellulose	1635:1647	arg1	epitopes					1649:1656	hemicellulose epitopes	1635:1656	hemicellulose epitopes	1635:1656	These studies showed different cellulose structural changes and differences in hemicellulose epitopes between switchgrass pretreatments with the aforementioned ILs.
25136131	0	29	theme	Efficient	0:8	arg1	pretreatment					18:29	Efficient biomass pretreatment	0:29	Efficient biomass pretreatment	0:29	Efficient biomass pretreatment using ionic liquids derived from lignin and hemicellulose.
25136131	9	30	theme	powder	1482:1487	arg1	diffraction					1495:1505	powder X-ray diffraction	1482:1505	powder X-ray diffraction	1482:1505	Effective pretreatment of lignocellulose was further investigated by powder X-ray diffraction and glycome profiling of switchgrass cell walls.
25136131	3	31	theme	imidazolium	371:381	arg1	cations					383:389	imidazolium cations	371:389	imidazolium cations	371:389	Although efficient, imidazolium cations are expensive and thus limited in their large-scale industrial deployment.
25136131	7	32	theme	72-h	1277:1280	arg1	incubation					1282:1291	72-h incubation	1277:1291	72-h incubation	1277:1291	Although the rate of cellulose hydrolysis for switchgrass pretreated with biomass-derived ILs was slightly slower than that of 1-ethyl-3-methylimidazolium acetate, 90-95% glucose and 70-75% xylose yields were obtained for these samples after 72-h incubation.
25136131	6	33	theme	yields	940:945	arg1	comparison					920:929	direct comparison	913:929	direct comparison of sugar yields and lignin removal between biomass-derived ILs and 1-ethyl-3-methylimidazolium acetate	913:1032	Enzymatic hydrolysis of pretreated switchgrass allowed for direct comparison of sugar yields and lignin removal between biomass-derived ILs and 1-ethyl-3-methylimidazolium acetate.
25136131	4	34	theme	amine-based	578:588	arg1	ILs					590:592	tertiary amine-based ILs	569:592	tertiary amine-based ILs from aromatic aldehydes derived from lignin and hemicellulose	569:654	To replace imidazolium-based ILs with those derived from renewable sources, we synthesized a series of tertiary amine-based ILs from aromatic aldehydes derived from lignin and hemicellulose, the major by-products of lignocellulosic biofuel production.
25136131	0	35	theme	ionic	37:41	arg1	liquids					43:49	ionic liquids	37:49	ionic liquids derived from lignin and hemicellulose	37:87	Efficient biomass pretreatment using ionic liquids derived from lignin and hemicellulose.
25136131	9	36	theme	glycome	1511:1517	arg1	profiling					1519:1527	glycome profiling	1511:1527	glycome profiling	1511:1527	Effective pretreatment of lignocellulose was further investigated by powder X-ray diffraction and glycome profiling of switchgrass cell walls.
25136131	11	37	attach	deriving	1736:1743	arg2	concept					1725:1731	Our concept	1721:1731	Our concept of deriving ILs from lignocellulosic biomass	1721:1776	Our concept of deriving ILs from lignocellulosic biomass shows significant potential for the realization of a "closed-loop" process for future lignocellulosic biorefineries and has far-reaching economic impacts for other IL-based process technology currently using ILs synthesized from petroleum sources.
25136131	11	37	attach	deriving	1736:1743	arg1	biomass					1770:1776	lignocellulosic biomass	1754:1776	lignocellulosic biomass	1754:1776	Our concept of deriving ILs from lignocellulosic biomass shows significant potential for the realization of a "closed-loop" process for future lignocellulosic biorefineries and has far-reaching economic impacts for other IL-based process technology currently using ILs synthesized from petroleum sources.
25136131	11	38	theme	far-reaching	1902:1913	arg1	impacts					1924:1930	far-reaching economic impacts	1902:1930	far-reaching economic impacts for other IL-based process technology currently using ILs synthesized from petroleum sources	1902:2023	Our concept of deriving ILs from lignocellulosic biomass shows significant potential for the realization of a "closed-loop" process for future lignocellulosic biorefineries and has far-reaching economic impacts for other IL-based process technology currently using ILs synthesized from petroleum sources.
25136131	6	39	theme	direct	913:918	arg1	comparison					920:929	direct comparison	913:929	direct comparison of sugar yields and lignin removal between biomass-derived ILs and 1-ethyl-3-methylimidazolium acetate	913:1032	Enzymatic hydrolysis of pretreated switchgrass allowed for direct comparison of sugar yields and lignin removal between biomass-derived ILs and 1-ethyl-3-methylimidazolium acetate.
25136131	11	40	theme	process	1951:1957	arg1	technology					1959:1968	other IL-based process technology	1936:1968	other IL-based process technology currently using ILs synthesized from petroleum sources	1936:2023	Our concept of deriving ILs from lignocellulosic biomass shows significant potential for the realization of a "closed-loop" process for future lignocellulosic biorefineries and has far-reaching economic impacts for other IL-based process technology currently using ILs synthesized from petroleum sources.
25136131	1	41	theme	process	185:191	arg1	chemistry					193:201	process chemistry	185:201	process chemistry	185:201	Ionic liquids (ILs), solvents composed entirely of paired ions, have been used in a variety of process chemistry and renewable energy applications.
25136131	11	42	theme	other	1936:1940	arg1	technology					1959:1968	other IL-based process technology	1936:1968	other IL-based process technology currently using ILs synthesized from petroleum sources	1936:2023	Our concept of deriving ILs from lignocellulosic biomass shows significant potential for the realization of a "closed-loop" process for future lignocellulosic biorefineries and has far-reaching economic impacts for other IL-based process technology currently using ILs synthesized from petroleum sources.
25136131	5	43	attach	derived	776:782	arg2	ILs					772:774	ILs	772:774	ILs derived from vanillin, p-anisaldehyde, and furfural	772:826	Compositional analysis of switchgrass pretreated with ILs derived from vanillin, p-anisaldehyde, and furfural confirmed their efficacy.
25136131	5	43	attach	derived	776:782	arg1	vanillin					789:796	vanillin	789:796	vanillin	789:796	Compositional analysis of switchgrass pretreated with ILs derived from vanillin, p-anisaldehyde, and furfural confirmed their efficacy.
25136131	5	43	attach	derived	776:782	arg1	p-anisaldehyde					799:812	p-anisaldehyde	799:812	p-anisaldehyde	799:812	Compositional analysis of switchgrass pretreated with ILs derived from vanillin, p-anisaldehyde, and furfural confirmed their efficacy.
25136131	5	43	attach	derived	776:782	arg1	furfural					819:826	furfural	819:826	furfural	819:826	Compositional analysis of switchgrass pretreated with ILs derived from vanillin, p-anisaldehyde, and furfural confirmed their efficacy.
25136131	6	44	theme	1-ethyl-3-methylimidazolium	998:1024	arg1	acetate					1026:1032	1-ethyl-3-methylimidazolium acetate	998:1032	1-ethyl-3-methylimidazolium acetate	998:1032	Enzymatic hydrolysis of pretreated switchgrass allowed for direct comparison of sugar yields and lignin removal between biomass-derived ILs and 1-ethyl-3-methylimidazolium acetate.
25136131	1	45	theme	energy	217:222	arg1	applications					224:235	renewable energy applications	207:235	renewable energy applications	207:235	Ionic liquids (ILs), solvents composed entirely of paired ions, have been used in a variety of process chemistry and renewable energy applications.
25136131	11	46	theme	"	1843:1843	arg1	process					1845:1851	a "closed-loop" process	1829:1851	a "closed-loop" process	1829:1851	Our concept of deriving ILs from lignocellulosic biomass shows significant potential for the realization of a "closed-loop" process for future lignocellulosic biorefineries and has far-reaching economic impacts for other IL-based process technology currently using ILs synthesized from petroleum sources.
25136131	7	47	theme	%	1223:1223	arg1	xylose					1225:1230	70-75% xylose	1218:1230	70-75% xylose	1218:1230	Although the rate of cellulose hydrolysis for switchgrass pretreated with biomass-derived ILs was slightly slower than that of 1-ethyl-3-methylimidazolium acetate, 90-95% glucose and 70-75% xylose yields were obtained for these samples after 72-h incubation.
25136131	6	48	theme	Enzymatic	854:862	arg1	hydrolysis					864:873	Enzymatic hydrolysis	854:873	Enzymatic hydrolysis of pretreated switchgrass	854:899	Enzymatic hydrolysis of pretreated switchgrass allowed for direct comparison of sugar yields and lignin removal between biomass-derived ILs and 1-ethyl-3-methylimidazolium acetate.
25136131	4	49	theme	production	706:715	arg1	series					559:564	a series	557:564	a series of tertiary amine-based ILs from aromatic aldehydes derived from lignin and hemicellulose	557:654	To replace imidazolium-based ILs with those derived from renewable sources, we synthesized a series of tertiary amine-based ILs from aromatic aldehydes derived from lignin and hemicellulose, the major by-products of lignocellulosic biofuel production.
25136131	4	49	theme	production	706:715	arg1	by-products					667:677	the major by-products	657:677	the major by-products of lignocellulosic biofuel production	657:715	To replace imidazolium-based ILs with those derived from renewable sources, we synthesized a series of tertiary amine-based ILs from aromatic aldehydes derived from lignin and hemicellulose, the major by-products of lignocellulosic biofuel production.
25136131	6	50	theme	lignin	951:956	arg1	removal					958:964	lignin removal	951:964	lignin removal	951:964	Enzymatic hydrolysis of pretreated switchgrass allowed for direct comparison of sugar yields and lignin removal between biomass-derived ILs and 1-ethyl-3-methylimidazolium acetate.
25136131	11	51	theme	lignocellulosic	1864:1878	arg1	biorefineries					1880:1892	future lignocellulosic biorefineries	1857:1892	future lignocellulosic biorefineries	1857:1892	Our concept of deriving ILs from lignocellulosic biomass shows significant potential for the realization of a "closed-loop" process for future lignocellulosic biorefineries and has far-reaching economic impacts for other IL-based process technology currently using ILs synthesized from petroleum sources.
25136131	7	52	theme	%	1204:1204	arg1	glucose					1206:1212	90-95% glucose	1199:1212	90-95% glucose	1199:1212	Although the rate of cellulose hydrolysis for switchgrass pretreated with biomass-derived ILs was slightly slower than that of 1-ethyl-3-methylimidazolium acetate, 90-95% glucose and 70-75% xylose yields were obtained for these samples after 72-h incubation.
25136131	10	53	theme	different	1577:1585	arg1	changes					1608:1614	different cellulose structural changes	1577:1614	different cellulose structural changes	1577:1614	These studies showed different cellulose structural changes and differences in hemicellulose epitopes between switchgrass pretreatments with the aforementioned ILs.
25136131	2	54	theme	remarkable	305:314	arg1	platform					316:323	a remarkable platform	303:323	a remarkable platform for biomass pretreatment	303:348	Imidazolium-based ILs effectively dissolve biomass and represent a remarkable platform for biomass pretreatment.
25136131	8	55	theme	Molecular	1294:1302	arg1	modeling					1304:1311	Molecular modeling	1294:1311	Molecular modeling	1294:1311	Molecular modeling was used to compare IL solvent parameters with experimentally obtained compositional analysis data.
25136131	10	56	from	changes	1608:1614	arg1	epitopes					1649:1656	hemicellulose epitopes	1635:1656	hemicellulose epitopes	1635:1656	These studies showed different cellulose structural changes and differences in hemicellulose epitopes between switchgrass pretreatments with the aforementioned ILs.
25136131	0	57	attach	derived	51:57	arg1	hemicellulose					75:87	hemicellulose	75:87	hemicellulose	75:87	Efficient biomass pretreatment using ionic liquids derived from lignin and hemicellulose.
25136131	0	57	attach	derived	51:57	arg2	liquids					43:49	ionic liquids	37:49	ionic liquids derived from lignin and hemicellulose	37:87	Efficient biomass pretreatment using ionic liquids derived from lignin and hemicellulose.
25136131	0	57	attach	derived	51:57	arg1	lignin					64:69	lignin	64:69	lignin	64:69	Efficient biomass pretreatment using ionic liquids derived from lignin and hemicellulose.
25136131	4	58	theme	biofuel	698:704	arg1	production					706:715	lignocellulosic biofuel production	682:715	lignocellulosic biofuel production	682:715	To replace imidazolium-based ILs with those derived from renewable sources, we synthesized a series of tertiary amine-based ILs from aromatic aldehydes derived from lignin and hemicellulose, the major by-products of lignocellulosic biofuel production.
25136131	5	59	theme	switchgrass	744:754	arg1	analysis					732:739	Compositional analysis	718:739	Compositional analysis of switchgrass pretreated with ILs derived from vanillin, p-anisaldehyde, and furfural	718:826	Compositional analysis of switchgrass pretreated with ILs derived from vanillin, p-anisaldehyde, and furfural confirmed their efficacy.
25136131	11	60	theme	lignocellulosic	1754:1768	arg1	biomass					1770:1776	lignocellulosic biomass	1754:1776	lignocellulosic biomass	1754:1776	Our concept of deriving ILs from lignocellulosic biomass shows significant potential for the realization of a "closed-loop" process for future lignocellulosic biorefineries and has far-reaching economic impacts for other IL-based process technology currently using ILs synthesized from petroleum sources.
25136131	10	61	from	differences	1620:1630	arg1	epitopes					1649:1656	hemicellulose epitopes	1635:1656	hemicellulose epitopes	1635:1656	These studies showed different cellulose structural changes and differences in hemicellulose epitopes between switchgrass pretreatments with the aforementioned ILs.
25136131	8	62	used	used	1317:1320	arg2	modeling					1304:1311	Molecular modeling	1294:1311	Molecular modeling	1294:1311	Molecular modeling was used to compare IL solvent parameters with experimentally obtained compositional analysis data.
25136131	8	63	theme	compositional	1384:1396	arg1	data					1407:1410	experimentally obtained compositional analysis data	1360:1410	experimentally obtained compositional analysis data	1360:1410	Molecular modeling was used to compare IL solvent parameters with experimentally obtained compositional analysis data.
25136131	7	64	theme	1-ethyl-3-methylimidazolium	1162:1188	arg1	acetate					1190:1196	1-ethyl-3-methylimidazolium acetate	1162:1196	1-ethyl-3-methylimidazolium acetate	1162:1196	Although the rate of cellulose hydrolysis for switchgrass pretreated with biomass-derived ILs was slightly slower than that of 1-ethyl-3-methylimidazolium acetate, 90-95% glucose and 70-75% xylose yields were obtained for these samples after 72-h incubation.
25136131	4	65	theme	imidazolium-based	477:493	arg1	ILs					495:497	imidazolium-based ILs	477:497	imidazolium-based ILs	477:497	To replace imidazolium-based ILs with those derived from renewable sources, we synthesized a series of tertiary amine-based ILs from aromatic aldehydes derived from lignin and hemicellulose, the major by-products of lignocellulosic biofuel production.
25136131	4	66	theme	major	661:665	arg1	series					559:564	a series	557:564	a series of tertiary amine-based ILs from aromatic aldehydes derived from lignin and hemicellulose	557:654	To replace imidazolium-based ILs with those derived from renewable sources, we synthesized a series of tertiary amine-based ILs from aromatic aldehydes derived from lignin and hemicellulose, the major by-products of lignocellulosic biofuel production.
25136131	4	66	theme	major	661:665	arg1	by-products					667:677	the major by-products	657:677	the major by-products of lignocellulosic biofuel production	657:715	To replace imidazolium-based ILs with those derived from renewable sources, we synthesized a series of tertiary amine-based ILs from aromatic aldehydes derived from lignin and hemicellulose, the major by-products of lignocellulosic biofuel production.
25136131	8	67	theme	IL	1333:1334	arg1	parameters					1344:1353	IL solvent parameters	1333:1353	IL solvent parameters	1333:1353	Molecular modeling was used to compare IL solvent parameters with experimentally obtained compositional analysis data.
25136131	2	68	theme	Imidazolium-based	238:254	arg1	ILs					256:258	Imidazolium-based ILs	238:258	Imidazolium-based ILs	238:258	Imidazolium-based ILs effectively dissolve biomass and represent a remarkable platform for biomass pretreatment.
25136131	4	69	theme	renewable	523:531	arg1	sources					533:539	renewable sources	523:539	renewable sources	523:539	To replace imidazolium-based ILs with those derived from renewable sources, we synthesized a series of tertiary amine-based ILs from aromatic aldehydes derived from lignin and hemicellulose, the major by-products of lignocellulosic biofuel production.
25136131	9	70	theme	cell	1544:1547	arg1	walls					1549:1553	switchgrass cell walls	1532:1553	switchgrass cell walls	1532:1553	Effective pretreatment of lignocellulose was further investigated by powder X-ray diffraction and glycome profiling of switchgrass cell walls.
25136131	1	71	theme	paired	141:146	arg1	ions					148:151	paired ions	141:151	paired ions	141:151	Ionic liquids (ILs), solvents composed entirely of paired ions, have been used in a variety of process chemistry and renewable energy applications.
25136131	11	72	contain	has	1898:1900	arg1	concept					1725:1731	Our concept	1721:1731	Our concept of deriving ILs from lignocellulosic biomass	1721:1776	Our concept of deriving ILs from lignocellulosic biomass shows significant potential for the realization of a "closed-loop" process for future lignocellulosic biorefineries and has far-reaching economic impacts for other IL-based process technology currently using ILs synthesized from petroleum sources.
25136131	11	72	contain	has	1898:1900	arg2	impacts					1924:1930	far-reaching economic impacts	1902:1930	far-reaching economic impacts for other IL-based process technology currently using ILs synthesized from petroleum sources	1902:2023	Our concept of deriving ILs from lignocellulosic biomass shows significant potential for the realization of a "closed-loop" process for future lignocellulosic biorefineries and has far-reaching economic impacts for other IL-based process technology currently using ILs synthesized from petroleum sources.
25136131	9	73	theme	lignocellulose	1439:1452	arg1	pretreatment					1423:1434	Effective pretreatment	1413:1434	Effective pretreatment of lignocellulose	1413:1452	Effective pretreatment of lignocellulose was further investigated by powder X-ray diffraction and glycome profiling of switchgrass cell walls.
25136131	7	74	theme	hydrolysis	1066:1075	arg1	slower					1142:1147	slower	1142:1147	slower	1142:1147	Although the rate of cellulose hydrolysis for switchgrass pretreated with biomass-derived ILs was slightly slower than that of 1-ethyl-3-methylimidazolium acetate, 90-95% glucose and 70-75% xylose yields were obtained for these samples after 72-h incubation.
25136131	7	74	theme	hydrolysis	1066:1075	arg1	rate					1048:1051	the rate	1044:1051	the rate of cellulose hydrolysis for switchgrass pretreated with biomass-derived ILs	1044:1127	Although the rate of cellulose hydrolysis for switchgrass pretreated with biomass-derived ILs was slightly slower than that of 1-ethyl-3-methylimidazolium acetate, 90-95% glucose and 70-75% xylose yields were obtained for these samples after 72-h incubation.
25136131	10	75	theme	structural	1597:1606	arg1	changes					1608:1614	different cellulose structural changes	1577:1614	different cellulose structural changes	1577:1614	These studies showed different cellulose structural changes and differences in hemicellulose epitopes between switchgrass pretreatments with the aforementioned ILs.
25136131	1	76	theme	chemistry	193:201	arg1	applications					224:235	renewable energy applications	207:235	renewable energy applications	207:235	Ionic liquids (ILs), solvents composed entirely of paired ions, have been used in a variety of process chemistry and renewable energy applications.
25136131	1	76	theme	chemistry	193:201	arg1	variety					174:180	a variety	172:180	a variety of process chemistry and renewable energy applications	172:235	Ionic liquids (ILs), solvents composed entirely of paired ions, have been used in a variety of process chemistry and renewable energy applications.
25136131	1	76	theme	chemistry	193:201	arg1	chemistry					193:201	process chemistry	185:201	process chemistry	185:201	Ionic liquids (ILs), solvents composed entirely of paired ions, have been used in a variety of process chemistry and renewable energy applications.
25136131	6	77	link	biomass-derived	974:988	arg1	ILs					990:992	biomass-derived ILs	974:992	biomass-derived ILs	974:992	Enzymatic hydrolysis of pretreated switchgrass allowed for direct comparison of sugar yields and lignin removal between biomass-derived ILs and 1-ethyl-3-methylimidazolium acetate.
25136131	0	78	theme	biomass	10:16	arg1	pretreatment					18:29	Efficient biomass pretreatment	0:29	Efficient biomass pretreatment	0:29	Efficient biomass pretreatment using ionic liquids derived from lignin and hemicellulose.
25136131	1	79	theme	Ionic	90:94	arg1	solvents					111:118	solvents	111:118	solvents composed entirely of paired ions	111:151	Ionic liquids (ILs), solvents composed entirely of paired ions, have been used in a variety of process chemistry and renewable energy applications.
25136131	1	79	theme	Ionic	90:94	arg1	liquids					96:102	Ionic liquids	90:102	Ionic liquids (ILs)	90:108	Ionic liquids (ILs), solvents composed entirely of paired ions, have been used in a variety of process chemistry and renewable energy applications.
25136131	1	79	theme	Ionic	90:94	arg1	ILs					105:107	ILs	105:107	ILs	105:107	Ionic liquids (ILs), solvents composed entirely of paired ions, have been used in a variety of process chemistry and renewable energy applications.
25136131	4	80	theme	tertiary	569:576	arg1	ILs					590:592	tertiary amine-based ILs	569:592	tertiary amine-based ILs from aromatic aldehydes derived from lignin and hemicellulose	569:654	To replace imidazolium-based ILs with those derived from renewable sources, we synthesized a series of tertiary amine-based ILs from aromatic aldehydes derived from lignin and hemicellulose, the major by-products of lignocellulosic biofuel production.
25136131	3	81	theme	large-scale	431:441	arg1	deployment					454:463	their large-scale industrial deployment	425:463	their large-scale industrial deployment	425:463	Although efficient, imidazolium cations are expensive and thus limited in their large-scale industrial deployment.
25136131	9	82	theme	X-ray	1489:1493	arg1	diffraction					1495:1505	powder X-ray diffraction	1482:1505	powder X-ray diffraction	1482:1505	Effective pretreatment of lignocellulose was further investigated by powder X-ray diffraction and glycome profiling of switchgrass cell walls.
25136131	4	83	theme	ILs	590:592	arg1	series					559:564	a series	557:564	a series of tertiary amine-based ILs from aromatic aldehydes derived from lignin and hemicellulose	557:654	To replace imidazolium-based ILs with those derived from renewable sources, we synthesized a series of tertiary amine-based ILs from aromatic aldehydes derived from lignin and hemicellulose, the major by-products of lignocellulosic biofuel production.
25136131	4	83	theme	ILs	590:592	arg1	by-products					667:677	the major by-products	657:677	the major by-products of lignocellulosic biofuel production	657:715	To replace imidazolium-based ILs with those derived from renewable sources, we synthesized a series of tertiary amine-based ILs from aromatic aldehydes derived from lignin and hemicellulose, the major by-products of lignocellulosic biofuel production.
25136131	11	84	theme	economic	1915:1922	arg1	impacts					1924:1930	far-reaching economic impacts	1902:1930	far-reaching economic impacts for other IL-based process technology currently using ILs synthesized from petroleum sources	1902:2023	Our concept of deriving ILs from lignocellulosic biomass shows significant potential for the realization of a "closed-loop" process for future lignocellulosic biorefineries and has far-reaching economic impacts for other IL-based process technology currently using ILs synthesized from petroleum sources.
25136131	4	85	from	series	559:564	arg1	aldehydes					608:616	aromatic aldehydes	599:616	aromatic aldehydes derived from lignin and hemicellulose	599:654	To replace imidazolium-based ILs with those derived from renewable sources, we synthesized a series of tertiary amine-based ILs from aromatic aldehydes derived from lignin and hemicellulose, the major by-products of lignocellulosic biofuel production.
25136131	6	86	theme	sugar	934:938	arg1	yields					940:945	sugar yields	934:945	sugar yields	934:945	Enzymatic hydrolysis of pretreated switchgrass allowed for direct comparison of sugar yields and lignin removal between biomass-derived ILs and 1-ethyl-3-methylimidazolium acetate.
25136131	10	87	theme	switchgrass	1666:1676	arg1	pretreatments					1678:1690	switchgrass pretreatments	1666:1690	switchgrass pretreatments with the aforementioned ILs	1666:1718	These studies showed different cellulose structural changes and differences in hemicellulose epitopes between switchgrass pretreatments with the aforementioned ILs.
25136131	11	88	theme	IL-based	1942:1949	arg1	technology					1959:1968	other IL-based process technology	1936:1968	other IL-based process technology currently using ILs synthesized from petroleum sources	1936:2023	Our concept of deriving ILs from lignocellulosic biomass shows significant potential for the realization of a "closed-loop" process for future lignocellulosic biorefineries and has far-reaching economic impacts for other IL-based process technology currently using ILs synthesized from petroleum sources.
25136131	11	89	theme	significant	1784:1794	arg1	potential					1796:1804	significant potential	1784:1804	significant potential for the realization of a "closed-loop" process for future lignocellulosic biorefineries	1784:1892	Our concept of deriving ILs from lignocellulosic biomass shows significant potential for the realization of a "closed-loop" process for future lignocellulosic biorefineries and has far-reaching economic impacts for other IL-based process technology currently using ILs synthesized from petroleum sources.
25136131	2	90	theme	biomass	329:335	arg1	pretreatment					337:348	biomass pretreatment	329:348	biomass pretreatment	329:348	Imidazolium-based ILs effectively dissolve biomass and represent a remarkable platform for biomass pretreatment.
25136131	9	91	theme	switchgrass	1532:1542	arg1	walls					1549:1553	switchgrass cell walls	1532:1553	switchgrass cell walls	1532:1553	Effective pretreatment of lignocellulose was further investigated by powder X-ray diffraction and glycome profiling of switchgrass cell walls.
25136131	10	92	theme	aforementioned	1701:1714	arg1	ILs					1716:1718	the aforementioned ILs	1697:1718	the aforementioned ILs	1697:1718	These studies showed different cellulose structural changes and differences in hemicellulose epitopes between switchgrass pretreatments with the aforementioned ILs.
25136131	9	93	theme	walls	1549:1553	arg1	diffraction					1495:1505	powder X-ray diffraction	1482:1505	powder X-ray diffraction	1482:1505	Effective pretreatment of lignocellulose was further investigated by powder X-ray diffraction and glycome profiling of switchgrass cell walls.
25136131	9	93	theme	walls	1549:1553	arg1	profiling					1519:1527	glycome profiling	1511:1527	glycome profiling	1511:1527	Effective pretreatment of lignocellulose was further investigated by powder X-ray diffraction and glycome profiling of switchgrass cell walls.
25136131	6	94	theme	switchgrass	889:899	arg1	hydrolysis					864:873	Enzymatic hydrolysis	854:873	Enzymatic hydrolysis of pretreated switchgrass	854:899	Enzymatic hydrolysis of pretreated switchgrass allowed for direct comparison of sugar yields and lignin removal between biomass-derived ILs and 1-ethyl-3-methylimidazolium acetate.
25136131	11	95	theme	process	1845:1851	arg1	realization					1814:1824	the realization	1810:1824	the realization of a "closed-loop" process	1810:1851	Our concept of deriving ILs from lignocellulosic biomass shows significant potential for the realization of a "closed-loop" process for future lignocellulosic biorefineries and has far-reaching economic impacts for other IL-based process technology currently using ILs synthesized from petroleum sources.
25136131	1	96	theme	renewable	207:215	arg1	applications					224:235	renewable energy applications	207:235	renewable energy applications	207:235	Ionic liquids (ILs), solvents composed entirely of paired ions, have been used in a variety of process chemistry and renewable energy applications.
27815124	8	0	theme	ion-exchange	700:711	arg1	chromatography					713:726	DEAE-Sepharose fast flow ion-exchange chromatography	675:726	DEAE-Sepharose fast flow ion-exchange chromatography	675:726	Three main fractions (HJP1, HJP2 and HJP3) were isolated using DEAE-Sepharose fast flow ion-exchange chromatography.
27815124	6	1	theme	assisted	497:504	arg1	method					517:522	an ultrasonically assisted extraction method	479:522	an ultrasonically assisted extraction method	479:522	Water-soluble polysaccharides were extracted using an ultrasonically assisted extraction method.
27815124	4	2	theme	physicochemical	318:332	arg1	characteristics					334:348	the physicochemical characteristics	314:348	the physicochemical characteristics	314:348	This work was designed to investigate the physicochemical characteristics and bioactivities of polysaccharides purified from Zizyphus jujuba cv.
27815124	10	3	theme	stronger	1035:1042	arg1	activities					1044:1053	stronger activities	1035:1053	stronger activities	1035:1053	The HJP and its three main fractions displayed DPPH radical scavenging activities as well as relatively strong reducing power and HJP had stronger activities than homogeneous compositions.
27815124	11	4	dep	cytokines	1282:1290	arg1	IL-6					1307:1310	IL-6	1307:1310	IL-6	1307:1310	Moreover, the results from in vitro immunological activities studies indicated that HJP could improve the phagocytosis activity of THP-l cells and had effect on the expression of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6).
27815124	11	4	dep	cytokines	1282:1290	arg1	IL-1β					1300:1304	IL-1β	1300:1304	IL-1β	1300:1304	Moreover, the results from in vitro immunological activities studies indicated that HJP could improve the phagocytosis activity of THP-l cells and had effect on the expression of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6).
27815124	11	4	dep	cytokines	1282:1290	arg1	TNF-α					1293:1297	TNF-α	1293:1297	TNF-α	1293:1297	Moreover, the results from in vitro immunological activities studies indicated that HJP could improve the phagocytosis activity of THP-l cells and had effect on the expression of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6).
27815124	9	5	theme	various	857:863	arg1	levels					865:870	various levels	857:870	various levels	857:870	The purified polysaccharides were found to consist of mannose, rhamnose, galactose, galacturonic acid, glucose and arabinose at various levels for different fractions.
27815124	13	6	contain	have	1399:1402	arg1	Muzao					1374:1378	Muzao	1374:1378	Muzao	1374:1378	Muzao were discovered to have antioxidative and immunological activities.
27815124	13	6	contain	have	1399:1402	arg2	activities					1436:1445	antioxidative and immunological activities	1404:1445	antioxidative and immunological activities	1404:1445	Muzao were discovered to have antioxidative and immunological activities.
27815124	6	7	theme	Water-soluble	428:440	arg1	polysaccharides					442:456	Water-soluble polysaccharides	428:456	Water-soluble polysaccharides	428:456	Water-soluble polysaccharides were extracted using an ultrasonically assisted extraction method.
27815124	11	8	from	studies	1147:1153	arg1	results					1100:1106	the results	1096:1106	the results from in vitro immunological activities studies	1096:1153	Moreover, the results from in vitro immunological activities studies indicated that HJP could improve the phagocytosis activity of THP-l cells and had effect on the expression of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6).
27815124	11	9	contain	had	1233:1235	arg1	results					1100:1106	the results	1096:1106	the results from in vitro immunological activities studies	1096:1153	Moreover, the results from in vitro immunological activities studies indicated that HJP could improve the phagocytosis activity of THP-l cells and had effect on the expression of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6).
27815124	11	9	contain	had	1233:1235	arg2	effect					1237:1242	effect	1237:1242	effect	1237:1242	Moreover, the results from in vitro immunological activities studies indicated that HJP could improve the phagocytosis activity of THP-l cells and had effect on the expression of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6).
27815124	2	10	theme	Chinese	150:156	arg1	jujuba					112:117	Zizyphus jujuba	103:117	Zizyphus jujuba	103:117	Zizyphus jujuba has been used as a traditional Chinese medicinal herb since ancient time.
27815124	2	10	theme	Chinese	150:156	arg1	herb					168:171	a traditional Chinese medicinal herb	136:171	a traditional Chinese medicinal herb since ancient time	136:190	Zizyphus jujuba has been used as a traditional Chinese medicinal herb since ancient time.
27815124	9	11	theme	different	876:884	arg1	fractions					886:894	different fractions	876:894	different fractions	876:894	The purified polysaccharides were found to consist of mannose, rhamnose, galactose, galacturonic acid, glucose and arabinose at various levels for different fractions.
27815124	2	12	theme	traditional	138:148	arg1	jujuba					112:117	Zizyphus jujuba	103:117	Zizyphus jujuba	103:117	Zizyphus jujuba has been used as a traditional Chinese medicinal herb since ancient time.
27815124	2	12	theme	traditional	138:148	arg1	herb					168:171	a traditional Chinese medicinal herb	136:171	a traditional Chinese medicinal herb since ancient time	136:190	Zizyphus jujuba has been used as a traditional Chinese medicinal herb since ancient time.
27815124	12	13	dep	polysaccharides	1333:1347	arg1	conclusion					1317:1326	conclusion	1317:1326	conclusion	1317:1326	In conclusion, the polysaccharides from Zizyphus jujuba cv.
27815124	11	14	theme	in	1113:1114	arg1	studies					1147:1153	in vitro immunological activities studies	1113:1153	in vitro immunological activities studies	1113:1153	Moreover, the results from in vitro immunological activities studies indicated that HJP could improve the phagocytosis activity of THP-l cells and had effect on the expression of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6).
27815124	12	15	theme	jujuba	1363:1368	arg1	cv					1370:1371	Zizyphus jujuba cv	1354:1371	Zizyphus jujuba cv	1354:1371	In conclusion, the polysaccharides from Zizyphus jujuba cv.
27815124	11	16	theme	activities	1136:1145	arg1	studies					1147:1153	in vitro immunological activities studies	1113:1153	in vitro immunological activities studies	1113:1153	Moreover, the results from in vitro immunological activities studies indicated that HJP could improve the phagocytosis activity of THP-l cells and had effect on the expression of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6).
27815124	0	17	from	activities	41:50	arg1	Jujuba					85:90	Jujuba	85:90	Jujuba	85:90	In vitro antioxidative and immunological activities of polysaccharides from Zizyphus Jujuba cv.
27815124	10	18	theme	scavenging	957:966	arg1	activities					968:977	DPPH radical scavenging activities	944:977	DPPH radical scavenging activities as well as relatively strong reducing power	944:1021	The HJP and its three main fractions displayed DPPH radical scavenging activities as well as relatively strong reducing power and HJP had stronger activities than homogeneous compositions.
27815124	3	19	theme	important	231:239	arg1	compounds					251:259	important bioactive compounds	231:259	important bioactive compounds	231:259	Polysaccharides have been found to be important bioactive compounds in the jujube.
27815124	10	20	theme	reducing	1008:1015	arg1	power					1017:1021	relatively strong reducing power	990:1021	DPPH radical scavenging activities as well as relatively strong reducing power	944:1021	The HJP and its three main fractions displayed DPPH radical scavenging activities as well as relatively strong reducing power and HJP had stronger activities than homogeneous compositions.
27815124	7	21	theme	purified	529:536	arg1	polysaccharides					538:552	The purified polysaccharides	525:552	The purified polysaccharides (HJP)	525:558	The purified polysaccharides (HJP) were obtained by deproteinization and decoloration.
27815124	7	21	theme	purified	529:536	arg1	HJP					555:557	HJP	555:557	HJP	555:557	The purified polysaccharides (HJP) were obtained by deproteinization and decoloration.
27815124	0	22	theme	In	0:1	arg1	activities					41:50	In vitro antioxidative and immunological activities	0:50	In vitro antioxidative and immunological activities of polysaccharides from Zizyphus Jujuba cv.	0:94	In vitro antioxidative and immunological activities of polysaccharides from Zizyphus Jujuba cv.
27815124	3	23	theme	bioactive	241:249	arg1	compounds					251:259	important bioactive compounds	231:259	important bioactive compounds	231:259	Polysaccharides have been found to be important bioactive compounds in the jujube.
27815124	4	24	theme	jujuba	410:415	arg1	cv					417:418	Zizyphus jujuba cv	401:418	Zizyphus jujuba cv	401:418	This work was designed to investigate the physicochemical characteristics and bioactivities of polysaccharides purified from Zizyphus jujuba cv.
27815124	10	25	theme	strong	1001:1006	arg1	power					1017:1021	relatively strong reducing power	990:1021	DPPH radical scavenging activities as well as relatively strong reducing power	944:1021	The HJP and its three main fractions displayed DPPH radical scavenging activities as well as relatively strong reducing power and HJP had stronger activities than homogeneous compositions.
27815124	11	26	theme	immunological	1122:1134	arg1	studies					1147:1153	in vitro immunological activities studies	1113:1153	in vitro immunological activities studies	1113:1153	Moreover, the results from in vitro immunological activities studies indicated that HJP could improve the phagocytosis activity of THP-l cells and had effect on the expression of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6).
27815124	4	27	theme	Zizyphus	401:408	arg1	cv					417:418	Zizyphus jujuba cv	401:418	Zizyphus jujuba cv	401:418	This work was designed to investigate the physicochemical characteristics and bioactivities of polysaccharides purified from Zizyphus jujuba cv.
27815124	11	28	theme	phagocytosis	1192:1203	arg1	activity					1205:1212	the phagocytosis activity	1188:1212	the phagocytosis activity of THP-l cells	1188:1227	Moreover, the results from in vitro immunological activities studies indicated that HJP could improve the phagocytosis activity of THP-l cells and had effect on the expression of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6).
27815124	0	29	theme	antioxidative	9:21	arg1	activities					41:50	In vitro antioxidative and immunological activities	0:50	In vitro antioxidative and immunological activities of polysaccharides from Zizyphus Jujuba cv.	0:94	In vitro antioxidative and immunological activities of polysaccharides from Zizyphus Jujuba cv.
27815124	3	30	from	compounds	251:259	arg1	jujube					268:273	the jujube	264:273	the jujube	264:273	Polysaccharides have been found to be important bioactive compounds in the jujube.
27815124	2	31	theme	ancient	179:185	arg1	time					187:190	ancient time	179:190	ancient time	179:190	Zizyphus jujuba has been used as a traditional Chinese medicinal herb since ancient time.
27815124	11	32	dep	in	1113:1114	arg1	vitro					1116:1120	vitro	1116:1120	vitro	1116:1120	Moreover, the results from in vitro immunological activities studies indicated that HJP could improve the phagocytosis activity of THP-l cells and had effect on the expression of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6).
27815124	10	33	theme	radical	949:955	arg1	scavenging					957:966	DPPH radical scavenging	944:966	DPPH radical scavenging activities as well as relatively strong reducing power	944:1021	The HJP and its three main fractions displayed DPPH radical scavenging activities as well as relatively strong reducing power and HJP had stronger activities than homogeneous compositions.
27815124	0	34	from	Jujuba	85:90	arg1	activities					41:50	In vitro antioxidative and immunological activities	0:50	In vitro antioxidative and immunological activities of polysaccharides from Zizyphus Jujuba cv.	0:94	In vitro antioxidative and immunological activities of polysaccharides from Zizyphus Jujuba cv.
27815124	0	34	from	Jujuba	85:90	arg1	polysaccharides					55:69	polysaccharides	55:69	polysaccharides from Zizyphus Jujuba cv	55:93	In vitro antioxidative and immunological activities of polysaccharides from Zizyphus Jujuba cv.
27815124	8	35	theme	main	618:621	arg1	fractions					623:631	Three main fractions	612:631	Three main fractions (HJP1, HJP2 and HJP3)	612:653	Three main fractions (HJP1, HJP2 and HJP3) were isolated using DEAE-Sepharose fast flow ion-exchange chromatography.
27815124	8	35	theme	main	618:621	arg1	HJP3					649:652	HJP3	649:652	HJP3	649:652	Three main fractions (HJP1, HJP2 and HJP3) were isolated using DEAE-Sepharose fast flow ion-exchange chromatography.
27815124	8	35	theme	main	618:621	arg1	HJP2					640:643	HJP2	640:643	HJP2	640:643	Three main fractions (HJP1, HJP2 and HJP3) were isolated using DEAE-Sepharose fast flow ion-exchange chromatography.
27815124	8	35	theme	main	618:621	arg1	HJP1					634:637	HJP1	634:637	HJP1	634:637	Three main fractions (HJP1, HJP2 and HJP3) were isolated using DEAE-Sepharose fast flow ion-exchange chromatography.
27815124	12	36	theme	Zizyphus	1354:1361	arg1	cv					1370:1371	Zizyphus jujuba cv	1354:1371	Zizyphus jujuba cv	1354:1371	In conclusion, the polysaccharides from Zizyphus jujuba cv.
27815124	0	37	theme	immunological	27:39	arg1	activities					41:50	In vitro antioxidative and immunological activities	0:50	In vitro antioxidative and immunological activities of polysaccharides from Zizyphus Jujuba cv.	0:94	In vitro antioxidative and immunological activities of polysaccharides from Zizyphus Jujuba cv.
27815124	2	38	theme	Zizyphus	103:110	arg1	jujuba					112:117	Zizyphus jujuba	103:117	Zizyphus jujuba	103:117	Zizyphus jujuba has been used as a traditional Chinese medicinal herb since ancient time.
27815124	2	38	theme	Zizyphus	103:110	arg1	herb					168:171	a traditional Chinese medicinal herb	136:171	a traditional Chinese medicinal herb since ancient time	136:190	Zizyphus jujuba has been used as a traditional Chinese medicinal herb since ancient time.
27815124	9	39	theme	galacturonic	813:824	arg1	acid					826:829	galacturonic acid	813:829	galacturonic acid	813:829	The purified polysaccharides were found to consist of mannose, rhamnose, galactose, galacturonic acid, glucose and arabinose at various levels for different fractions.
27815124	0	40	theme	polysaccharides	55:69	arg1	activities					41:50	In vitro antioxidative and immunological activities	0:50	In vitro antioxidative and immunological activities of polysaccharides from Zizyphus Jujuba cv.	0:94	In vitro antioxidative and immunological activities of polysaccharides from Zizyphus Jujuba cv.
27815124	13	41	theme	immunological	1422:1434	arg1	activities					1436:1445	antioxidative and immunological activities	1404:1445	antioxidative and immunological activities	1404:1445	Muzao were discovered to have antioxidative and immunological activities.
27815124	2	42	theme	medicinal	158:166	arg1	jujuba					112:117	Zizyphus jujuba	103:117	Zizyphus jujuba	103:117	Zizyphus jujuba has been used as a traditional Chinese medicinal herb since ancient time.
27815124	2	42	theme	medicinal	158:166	arg1	herb					168:171	a traditional Chinese medicinal herb	136:171	a traditional Chinese medicinal herb since ancient time	136:190	Zizyphus jujuba has been used as a traditional Chinese medicinal herb since ancient time.
27815124	10	43	contain	had	1031:1033	arg2	activities					1044:1053	stronger activities	1035:1053	stronger activities	1035:1053	The HJP and its three main fractions displayed DPPH radical scavenging activities as well as relatively strong reducing power and HJP had stronger activities than homogeneous compositions.
27815124	10	43	contain	had	1031:1033	arg1	HJP					901:903	The HJP	897:903	The HJP	897:903	The HJP and its three main fractions displayed DPPH radical scavenging activities as well as relatively strong reducing power and HJP had stronger activities than homogeneous compositions.
27815124	10	44	theme	main	919:922	arg1	fractions					924:932	three main fractions	913:932	its three main fractions	909:932	The HJP and its three main fractions displayed DPPH radical scavenging activities as well as relatively strong reducing power and HJP had stronger activities than homogeneous compositions.
27815124	2	45	used	used	128:131	arg2	jujuba					112:117	Zizyphus jujuba	103:117	Zizyphus jujuba	103:117	Zizyphus jujuba has been used as a traditional Chinese medicinal herb since ancient time.
27815124	2	45	used	used	128:131	arg2	herb					168:171	a traditional Chinese medicinal herb	136:171	a traditional Chinese medicinal herb since ancient time	136:190	Zizyphus jujuba has been used as a traditional Chinese medicinal herb since ancient time.
27815124	12	46	from	cv	1370:1371	arg1	polysaccharides					1333:1347	the polysaccharides	1329:1347	the polysaccharides from Zizyphus jujuba cv	1329:1371	In conclusion, the polysaccharides from Zizyphus jujuba cv.
27815124	8	47	theme	DEAE-Sepharose	675:688	arg1	chromatography					713:726	DEAE-Sepharose fast flow ion-exchange chromatography	675:726	DEAE-Sepharose fast flow ion-exchange chromatography	675:726	Three main fractions (HJP1, HJP2 and HJP3) were isolated using DEAE-Sepharose fast flow ion-exchange chromatography.
27815124	11	48	theme	pro-inflammatory	1265:1280	arg1	cytokines					1282:1290	pro-inflammatory cytokines	1265:1290	pro-inflammatory cytokines (TNF-α, IL-1β, IL-6)	1265:1311	Moreover, the results from in vitro immunological activities studies indicated that HJP could improve the phagocytosis activity of THP-l cells and had effect on the expression of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6).
27815124	4	49	theme	polysaccharides	371:385	arg1	bioactivities					354:366	bioactivities	354:366	bioactivities	354:366	This work was designed to investigate the physicochemical characteristics and bioactivities of polysaccharides purified from Zizyphus jujuba cv.
27815124	4	49	theme	polysaccharides	371:385	arg1	characteristics					334:348	the physicochemical characteristics	314:348	the physicochemical characteristics	314:348	This work was designed to investigate the physicochemical characteristics and bioactivities of polysaccharides purified from Zizyphus jujuba cv.
27815124	9	50	from	levels	865:870	arg1	acid					826:829	galacturonic acid	813:829	galacturonic acid	813:829	The purified polysaccharides were found to consist of mannose, rhamnose, galactose, galacturonic acid, glucose and arabinose at various levels for different fractions.
27815124	9	50	from	levels	865:870	arg1	rhamnose					792:799	rhamnose	792:799	rhamnose	792:799	The purified polysaccharides were found to consist of mannose, rhamnose, galactose, galacturonic acid, glucose and arabinose at various levels for different fractions.
27815124	9	50	from	levels	865:870	arg1	glucose					832:838	glucose	832:838	glucose	832:838	The purified polysaccharides were found to consist of mannose, rhamnose, galactose, galacturonic acid, glucose and arabinose at various levels for different fractions.
27815124	9	50	from	levels	865:870	arg1	galactose					802:810	galactose	802:810	galactose	802:810	The purified polysaccharides were found to consist of mannose, rhamnose, galactose, galacturonic acid, glucose and arabinose at various levels for different fractions.
27815124	9	50	from	levels	865:870	arg1	arabinose					844:852	arabinose	844:852	arabinose	844:852	The purified polysaccharides were found to consist of mannose, rhamnose, galactose, galacturonic acid, glucose and arabinose at various levels for different fractions.
27815124	9	50	from	levels	865:870	arg1	mannose					783:789	mannose	783:789	mannose	783:789	The purified polysaccharides were found to consist of mannose, rhamnose, galactose, galacturonic acid, glucose and arabinose at various levels for different fractions.
27815124	11	51	theme	cells	1223:1227	arg1	activity					1205:1212	the phagocytosis activity	1188:1212	the phagocytosis activity of THP-l cells	1188:1227	Moreover, the results from in vitro immunological activities studies indicated that HJP could improve the phagocytosis activity of THP-l cells and had effect on the expression of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6).
27815124	10	52	theme	homogeneous	1060:1070	arg1	compositions					1072:1083	homogeneous compositions	1060:1083	homogeneous compositions	1060:1083	The HJP and its three main fractions displayed DPPH radical scavenging activities as well as relatively strong reducing power and HJP had stronger activities than homogeneous compositions.
27815124	6	53	theme	extraction	506:515	arg1	method					517:522	an ultrasonically assisted extraction method	479:522	an ultrasonically assisted extraction method	479:522	Water-soluble polysaccharides were extracted using an ultrasonically assisted extraction method.
27815124	8	54	theme	flow	695:698	arg1	chromatography					713:726	DEAE-Sepharose fast flow ion-exchange chromatography	675:726	DEAE-Sepharose fast flow ion-exchange chromatography	675:726	Three main fractions (HJP1, HJP2 and HJP3) were isolated using DEAE-Sepharose fast flow ion-exchange chromatography.
27815124	10	55	theme	DPPH	944:947	arg1	scavenging					957:966	DPPH radical scavenging	944:966	DPPH radical scavenging activities as well as relatively strong reducing power	944:1021	The HJP and its three main fractions displayed DPPH radical scavenging activities as well as relatively strong reducing power and HJP had stronger activities than homogeneous compositions.
27815124	11	56	theme	cytokines	1282:1290	arg1	expression					1251:1260	the expression	1247:1260	the expression of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6)	1247:1311	Moreover, the results from in vitro immunological activities studies indicated that HJP could improve the phagocytosis activity of THP-l cells and had effect on the expression of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6).
27815124	8	57	theme	fast	690:693	arg1	chromatography					713:726	DEAE-Sepharose fast flow ion-exchange chromatography	675:726	DEAE-Sepharose fast flow ion-exchange chromatography	675:726	Three main fractions (HJP1, HJP2 and HJP3) were isolated using DEAE-Sepharose fast flow ion-exchange chromatography.
27815124	9	58	theme	purified	733:740	arg1	polysaccharides					742:756	The purified polysaccharides	729:756	The purified polysaccharides	729:756	The purified polysaccharides were found to consist of mannose, rhamnose, galactose, galacturonic acid, glucose and arabinose at various levels for different fractions.
27815124	11	59	theme	THP-l	1217:1221	arg1	cells					1223:1227	THP-l cells	1217:1227	THP-l cells	1217:1227	Moreover, the results from in vitro immunological activities studies indicated that HJP could improve the phagocytosis activity of THP-l cells and had effect on the expression of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6).
27815124	0	60	dep	In	0:1	arg1	vitro					3:7	vitro	3:7	vitro	3:7	In vitro antioxidative and immunological activities of polysaccharides from Zizyphus Jujuba cv.
27815124	8	61	dep	fractions	623:631	arg1	fractions					623:631	Three main fractions	612:631	Three main fractions (HJP1, HJP2 and HJP3)	612:653	Three main fractions (HJP1, HJP2 and HJP3) were isolated using DEAE-Sepharose fast flow ion-exchange chromatography.
27815124	8	61	dep	fractions	623:631	arg1	HJP3					649:652	HJP3	649:652	HJP3	649:652	Three main fractions (HJP1, HJP2 and HJP3) were isolated using DEAE-Sepharose fast flow ion-exchange chromatography.
27815124	8	61	dep	fractions	623:631	arg1	HJP2					640:643	HJP2	640:643	HJP2	640:643	Three main fractions (HJP1, HJP2 and HJP3) were isolated using DEAE-Sepharose fast flow ion-exchange chromatography.
27815124	8	61	dep	fractions	623:631	arg1	HJP1					634:637	HJP1	634:637	HJP1	634:637	Three main fractions (HJP1, HJP2 and HJP3) were isolated using DEAE-Sepharose fast flow ion-exchange chromatography.
27815124	13	62	theme	antioxidative	1404:1416	arg1	activities					1436:1445	antioxidative and immunological activities	1404:1445	antioxidative and immunological activities	1404:1445	Muzao were discovered to have antioxidative and immunological activities.
27474571	2	0	theme	high-performance	410:425	arg1	chromatography					442:455	high-performance anion exchange chromatography	410:455	high-performance anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD) and fourier transform infrared spectrometer (FT-IR) as well	410:570	The physicochemical properties were detected by high-performance gel permeation chromatography (HPGPC), high-performance anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD) and fourier transform infrared spectrometer (FT-IR) as well.
27474571	2	0	theme	high-performance	410:425	arg1	chromatography					386:399	high-performance gel permeation chromatography	354:399	high-performance gel permeation chromatography (HPGPC)	354:407	The physicochemical properties were detected by high-performance gel permeation chromatography (HPGPC), high-performance anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD) and fourier transform infrared spectrometer (FT-IR) as well.
27474571	4	1	theme	CP	1091:1092	arg1	composition					1076:1086	monosaccharide composition	1061:1086	monosaccharide composition of CP (1.0:4.6:3.5:4.5) and UCP (1.0:5.9:3.9:4.4)	1061:1136	Results showed that no considerable change was observed on the structure between the polysaccharide of Cyclocarya paliurus (CP) and the ultrasonic polysaccharide of Cyclocarya paliurus (UCP) by FT-IR, and no apparent differences were found on the content of sugar, uronic acid and protein after the ultrasonic treatment, but difference appeared in the ratio of monosaccharide composition of CP (1.0:4.6:3.5:4.5) and UCP (1.0:5.9:3.9:4.4), and the antioxidant activities of CP were promoted after ultrasonic treatment.
27474571	4	2	theme	apparent	908:915	arg1	differences					917:927	no apparent differences	905:927	no apparent differences	905:927	Results showed that no considerable change was observed on the structure between the polysaccharide of Cyclocarya paliurus (CP) and the ultrasonic polysaccharide of Cyclocarya paliurus (UCP) by FT-IR, and no apparent differences were found on the content of sugar, uronic acid and protein after the ultrasonic treatment, but difference appeared in the ratio of monosaccharide composition of CP (1.0:4.6:3.5:4.5) and UCP (1.0:5.9:3.9:4.4), and the antioxidant activities of CP were promoted after ultrasonic treatment.
27474571	3	3	theme	antioxidant	577:587	arg1	activities					589:598	The antioxidant activities	573:598	The antioxidant activities	573:598	The antioxidant activities were evaluated by the assay of DPPH, hydroxyl free radical, β-carotene-linoleic acid, respectfully.
27474571	0	4	from	Effect	0:5	arg1	activities					81:90	antioxidant activities	69:90	antioxidant activities	69:90	Effect of ultrasonic treatment on the physicochemical properties and antioxidant activities of polysaccharide from Cyclocarya paliurus.
27474571	0	4	from	Effect	0:5	arg1	properties					54:63	physicochemical properties	38:63	physicochemical properties	38:63	Effect of ultrasonic treatment on the physicochemical properties and antioxidant activities of polysaccharide from Cyclocarya paliurus.
27474571	0	5	theme	polysaccharide	95:108	arg1	activities					81:90	antioxidant activities	69:90	antioxidant activities	69:90	Effect of ultrasonic treatment on the physicochemical properties and antioxidant activities of polysaccharide from Cyclocarya paliurus.
27474571	0	5	theme	polysaccharide	95:108	arg1	properties					54:63	physicochemical properties	38:63	physicochemical properties	38:63	Effect of ultrasonic treatment on the physicochemical properties and antioxidant activities of polysaccharide from Cyclocarya paliurus.
27474571	0	6	from	properties	54:63	arg1	paliurus					126:133	Cyclocarya paliurus	115:133	Cyclocarya paliurus	115:133	Effect of ultrasonic treatment on the physicochemical properties and antioxidant activities of polysaccharide from Cyclocarya paliurus.
27474571	4	7	located	observed	747:754	arg1	structure					763:771	the structure	759:771	the structure between the polysaccharide of Cyclocarya paliurus (CP) and the ultrasonic polysaccharide of Cyclocarya paliurus (UCP) by FT-IR	759:898	Results showed that no considerable change was observed on the structure between the polysaccharide of Cyclocarya paliurus (CP) and the ultrasonic polysaccharide of Cyclocarya paliurus (UCP) by FT-IR, and no apparent differences were found on the content of sugar, uronic acid and protein after the ultrasonic treatment, but difference appeared in the ratio of monosaccharide composition of CP (1.0:4.6:3.5:4.5) and UCP (1.0:5.9:3.9:4.4), and the antioxidant activities of CP were promoted after ultrasonic treatment.
27474571	4	7	located	observed	747:754	arg2	change					736:741	no considerable change	720:741	no considerable change	720:741	Results showed that no considerable change was observed on the structure between the polysaccharide of Cyclocarya paliurus (CP) and the ultrasonic polysaccharide of Cyclocarya paliurus (UCP) by FT-IR, and no apparent differences were found on the content of sugar, uronic acid and protein after the ultrasonic treatment, but difference appeared in the ratio of monosaccharide composition of CP (1.0:4.6:3.5:4.5) and UCP (1.0:5.9:3.9:4.4), and the antioxidant activities of CP were promoted after ultrasonic treatment.
27474571	4	8	theme	ultrasonic	999:1008	arg1	treatment					1010:1018	the ultrasonic treatment	995:1018	the ultrasonic treatment	995:1018	Results showed that no considerable change was observed on the structure between the polysaccharide of Cyclocarya paliurus (CP) and the ultrasonic polysaccharide of Cyclocarya paliurus (UCP) by FT-IR, and no apparent differences were found on the content of sugar, uronic acid and protein after the ultrasonic treatment, but difference appeared in the ratio of monosaccharide composition of CP (1.0:4.6:3.5:4.5) and UCP (1.0:5.9:3.9:4.4), and the antioxidant activities of CP were promoted after ultrasonic treatment.
27474571	4	9	theme	antioxidant	1147:1157	arg1	activities					1159:1168	the antioxidant activities	1143:1168	the antioxidant activities of CP	1143:1174	Results showed that no considerable change was observed on the structure between the polysaccharide of Cyclocarya paliurus (CP) and the ultrasonic polysaccharide of Cyclocarya paliurus (UCP) by FT-IR, and no apparent differences were found on the content of sugar, uronic acid and protein after the ultrasonic treatment, but difference appeared in the ratio of monosaccharide composition of CP (1.0:4.6:3.5:4.5) and UCP (1.0:5.9:3.9:4.4), and the antioxidant activities of CP were promoted after ultrasonic treatment.
27474571	1	10	theme	physicochemical	179:193	arg1	properties					195:204	physicochemical properties	179:204	physicochemical properties	179:204	The effects of ultrasonic treatment on the physicochemical properties and antioxidant activities of the polysaccharide from Cyclocarya paliurus leaves were investigated.
27474571	0	11	theme	Cyclocarya	115:124	arg1	paliurus					126:133	Cyclocarya paliurus	115:133	Cyclocarya paliurus	115:133	Effect of ultrasonic treatment on the physicochemical properties and antioxidant activities of polysaccharide from Cyclocarya paliurus.
27474571	1	12	from	effects	140:146	arg1	properties					195:204	physicochemical properties	179:204	physicochemical properties	179:204	The effects of ultrasonic treatment on the physicochemical properties and antioxidant activities of the polysaccharide from Cyclocarya paliurus leaves were investigated.
27474571	1	12	from	effects	140:146	arg1	activities					222:231	antioxidant activities	210:231	antioxidant activities	210:231	The effects of ultrasonic treatment on the physicochemical properties and antioxidant activities of the polysaccharide from Cyclocarya paliurus leaves were investigated.
27474571	2	13	theme	physicochemical	310:324	arg1	properties					326:335	The physicochemical properties	306:335	The physicochemical properties	306:335	The physicochemical properties were detected by high-performance gel permeation chromatography (HPGPC), high-performance anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD) and fourier transform infrared spectrometer (FT-IR) as well.
27474571	4	14	theme	CP	1173:1174	arg1	activities					1159:1168	the antioxidant activities	1143:1168	the antioxidant activities of CP	1143:1174	Results showed that no considerable change was observed on the structure between the polysaccharide of Cyclocarya paliurus (CP) and the ultrasonic polysaccharide of Cyclocarya paliurus (UCP) by FT-IR, and no apparent differences were found on the content of sugar, uronic acid and protein after the ultrasonic treatment, but difference appeared in the ratio of monosaccharide composition of CP (1.0:4.6:3.5:4.5) and UCP (1.0:5.9:3.9:4.4), and the antioxidant activities of CP were promoted after ultrasonic treatment.
27474571	4	15	theme	considerable	723:734	arg1	change					736:741	no considerable change	720:741	no considerable change	720:741	Results showed that no considerable change was observed on the structure between the polysaccharide of Cyclocarya paliurus (CP) and the ultrasonic polysaccharide of Cyclocarya paliurus (UCP) by FT-IR, and no apparent differences were found on the content of sugar, uronic acid and protein after the ultrasonic treatment, but difference appeared in the ratio of monosaccharide composition of CP (1.0:4.6:3.5:4.5) and UCP (1.0:5.9:3.9:4.4), and the antioxidant activities of CP were promoted after ultrasonic treatment.
27474571	4	16	theme	monosaccharide	1061:1074	arg1	composition					1076:1086	monosaccharide composition	1061:1086	monosaccharide composition of CP (1.0:4.6:3.5:4.5) and UCP (1.0:5.9:3.9:4.4)	1061:1136	Results showed that no considerable change was observed on the structure between the polysaccharide of Cyclocarya paliurus (CP) and the ultrasonic polysaccharide of Cyclocarya paliurus (UCP) by FT-IR, and no apparent differences were found on the content of sugar, uronic acid and protein after the ultrasonic treatment, but difference appeared in the ratio of monosaccharide composition of CP (1.0:4.6:3.5:4.5) and UCP (1.0:5.9:3.9:4.4), and the antioxidant activities of CP were promoted after ultrasonic treatment.
27474571	2	17	dep	transform	524:532	arg1	infrared					534:541	infrared	534:541	transform infrared spectrometer (FT-IR) as well	524:570	The physicochemical properties were detected by high-performance gel permeation chromatography (HPGPC), high-performance anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD) and fourier transform infrared spectrometer (FT-IR) as well.
27474571	2	18	theme	permeation	375:384	arg1	HPGPC					402:406	HPGPC	402:406	HPGPC	402:406	The physicochemical properties were detected by high-performance gel permeation chromatography (HPGPC), high-performance anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD) and fourier transform infrared spectrometer (FT-IR) as well.
27474571	2	18	theme	permeation	375:384	arg1	chromatography					386:399	high-performance gel permeation chromatography	354:399	high-performance gel permeation chromatography (HPGPC)	354:407	The physicochemical properties were detected by high-performance gel permeation chromatography (HPGPC), high-performance anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD) and fourier transform infrared spectrometer (FT-IR) as well.
27474571	2	18	theme	permeation	375:384	arg1	chromatography					442:455	high-performance anion exchange chromatography	410:455	high-performance anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD) and fourier transform infrared spectrometer (FT-IR) as well	410:570	The physicochemical properties were detected by high-performance gel permeation chromatography (HPGPC), high-performance anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD) and fourier transform infrared spectrometer (FT-IR) as well.
27474571	2	19	dep	fourier	516:522	arg1	transform					524:532	transform	524:532	transform infrared spectrometer (FT-IR) as well	524:570	The physicochemical properties were detected by high-performance gel permeation chromatography (HPGPC), high-performance anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD) and fourier transform infrared spectrometer (FT-IR) as well.
27474571	2	20	theme	gel	371:373	arg1	HPGPC					402:406	HPGPC	402:406	HPGPC	402:406	The physicochemical properties were detected by high-performance gel permeation chromatography (HPGPC), high-performance anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD) and fourier transform infrared spectrometer (FT-IR) as well.
27474571	2	20	theme	gel	371:373	arg1	chromatography					386:399	high-performance gel permeation chromatography	354:399	high-performance gel permeation chromatography (HPGPC)	354:407	The physicochemical properties were detected by high-performance gel permeation chromatography (HPGPC), high-performance anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD) and fourier transform infrared spectrometer (FT-IR) as well.
27474571	2	20	theme	gel	371:373	arg1	chromatography					442:455	high-performance anion exchange chromatography	410:455	high-performance anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD) and fourier transform infrared spectrometer (FT-IR) as well	410:570	The physicochemical properties were detected by high-performance gel permeation chromatography (HPGPC), high-performance anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD) and fourier transform infrared spectrometer (FT-IR) as well.
27474571	1	21	theme	antioxidant	210:220	arg1	activities					222:231	antioxidant activities	210:231	antioxidant activities	210:231	The effects of ultrasonic treatment on the physicochemical properties and antioxidant activities of the polysaccharide from Cyclocarya paliurus leaves were investigated.
27474571	0	22	theme	treatment	21:29	arg1	Effect					0:5	Effect	0:5	Effect of ultrasonic treatment on the physicochemical properties and antioxidant activities of polysaccharide from Cyclocarya paliurus.	0:134	Effect of ultrasonic treatment on the physicochemical properties and antioxidant activities of polysaccharide from Cyclocarya paliurus.
27474571	4	23	theme	paliurus	814:821	arg1	polysaccharide					785:798	the polysaccharide	781:798	the polysaccharide of Cyclocarya paliurus (CP)	781:826	Results showed that no considerable change was observed on the structure between the polysaccharide of Cyclocarya paliurus (CP) and the ultrasonic polysaccharide of Cyclocarya paliurus (UCP) by FT-IR, and no apparent differences were found on the content of sugar, uronic acid and protein after the ultrasonic treatment, but difference appeared in the ratio of monosaccharide composition of CP (1.0:4.6:3.5:4.5) and UCP (1.0:5.9:3.9:4.4), and the antioxidant activities of CP were promoted after ultrasonic treatment.
27474571	4	23	theme	paliurus	814:821	arg1	polysaccharide					847:860	the ultrasonic polysaccharide	832:860	the ultrasonic polysaccharide of Cyclocarya paliurus (UCP) by FT-IR	832:898	Results showed that no considerable change was observed on the structure between the polysaccharide of Cyclocarya paliurus (CP) and the ultrasonic polysaccharide of Cyclocarya paliurus (UCP) by FT-IR, and no apparent differences were found on the content of sugar, uronic acid and protein after the ultrasonic treatment, but difference appeared in the ratio of monosaccharide composition of CP (1.0:4.6:3.5:4.5) and UCP (1.0:5.9:3.9:4.4), and the antioxidant activities of CP were promoted after ultrasonic treatment.
27474571	4	24	theme	uronic	965:970	arg1	acid					972:975	uronic acid	965:975	uronic acid	965:975	Results showed that no considerable change was observed on the structure between the polysaccharide of Cyclocarya paliurus (CP) and the ultrasonic polysaccharide of Cyclocarya paliurus (UCP) by FT-IR, and no apparent differences were found on the content of sugar, uronic acid and protein after the ultrasonic treatment, but difference appeared in the ratio of monosaccharide composition of CP (1.0:4.6:3.5:4.5) and UCP (1.0:5.9:3.9:4.4), and the antioxidant activities of CP were promoted after ultrasonic treatment.
27474571	2	25	theme	high-performance	354:369	arg1	HPGPC					402:406	HPGPC	402:406	HPGPC	402:406	The physicochemical properties were detected by high-performance gel permeation chromatography (HPGPC), high-performance anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD) and fourier transform infrared spectrometer (FT-IR) as well.
27474571	2	25	theme	high-performance	354:369	arg1	chromatography					386:399	high-performance gel permeation chromatography	354:399	high-performance gel permeation chromatography (HPGPC)	354:407	The physicochemical properties were detected by high-performance gel permeation chromatography (HPGPC), high-performance anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD) and fourier transform infrared spectrometer (FT-IR) as well.
27474571	2	25	theme	high-performance	354:369	arg1	chromatography					442:455	high-performance anion exchange chromatography	410:455	high-performance anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD) and fourier transform infrared spectrometer (FT-IR) as well	410:570	The physicochemical properties were detected by high-performance gel permeation chromatography (HPGPC), high-performance anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD) and fourier transform infrared spectrometer (FT-IR) as well.
27474571	0	26	theme	ultrasonic	10:19	arg1	treatment					21:29	ultrasonic treatment	10:29	ultrasonic treatment	10:29	Effect of ultrasonic treatment on the physicochemical properties and antioxidant activities of polysaccharide from Cyclocarya paliurus.
27474571	3	27	theme	DPPH	631:634	arg1	assay					622:626	the assay	618:626	the assay of DPPH, hydroxyl free radical, β-carotene-linoleic acid, respectfully	618:697	The antioxidant activities were evaluated by the assay of DPPH, hydroxyl free radical, β-carotene-linoleic acid, respectfully.
27474571	2	28	theme	exchange	433:440	arg1	chromatography					442:455	high-performance anion exchange chromatography	410:455	high-performance anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD) and fourier transform infrared spectrometer (FT-IR) as well	410:570	The physicochemical properties were detected by high-performance gel permeation chromatography (HPGPC), high-performance anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD) and fourier transform infrared spectrometer (FT-IR) as well.
27474571	2	28	theme	exchange	433:440	arg1	chromatography					386:399	high-performance gel permeation chromatography	354:399	high-performance gel permeation chromatography (HPGPC)	354:407	The physicochemical properties were detected by high-performance gel permeation chromatography (HPGPC), high-performance anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD) and fourier transform infrared spectrometer (FT-IR) as well.
27474571	0	29	from	activities	81:90	arg1	paliurus					126:133	Cyclocarya paliurus	115:133	Cyclocarya paliurus	115:133	Effect of ultrasonic treatment on the physicochemical properties and antioxidant activities of polysaccharide from Cyclocarya paliurus.
27474571	0	30	dep	properties	54:63	arg1	the					34:36	the	34:36	the	34:36	Effect of ultrasonic treatment on the physicochemical properties and antioxidant activities of polysaccharide from Cyclocarya paliurus.
27474571	3	31	theme	hydroxyl	637:644	arg1	DPPH					631:634	DPPH	631:634	DPPH	631:634	The antioxidant activities were evaluated by the assay of DPPH, hydroxyl free radical, β-carotene-linoleic acid, respectfully.
27474571	3	31	theme	hydroxyl	637:644	arg1	radical					651:657	hydroxyl free radical	637:657	hydroxyl free radical	637:657	The antioxidant activities were evaluated by the assay of DPPH, hydroxyl free radical, β-carotene-linoleic acid, respectfully.
27474571	2	32	theme	pulsed	470:475	arg1	detection					490:498	pulsed amperometric detection	470:498	pulsed amperometric detection (HPAEC-PAD)	470:510	The physicochemical properties were detected by high-performance gel permeation chromatography (HPGPC), high-performance anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD) and fourier transform infrared spectrometer (FT-IR) as well.
27474571	2	32	theme	pulsed	470:475	arg1	HPAEC-PAD					501:509	HPAEC-PAD	501:509	HPAEC-PAD	501:509	The physicochemical properties were detected by high-performance gel permeation chromatography (HPGPC), high-performance anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD) and fourier transform infrared spectrometer (FT-IR) as well.
27474571	4	33	theme	acid	972:975	arg1	content					947:953	the content	943:953	the content of sugar, uronic acid and protein after the ultrasonic treatment	943:1018	Results showed that no considerable change was observed on the structure between the polysaccharide of Cyclocarya paliurus (CP) and the ultrasonic polysaccharide of Cyclocarya paliurus (UCP) by FT-IR, and no apparent differences were found on the content of sugar, uronic acid and protein after the ultrasonic treatment, but difference appeared in the ratio of monosaccharide composition of CP (1.0:4.6:3.5:4.5) and UCP (1.0:5.9:3.9:4.4), and the antioxidant activities of CP were promoted after ultrasonic treatment.
27474571	3	34	theme	β-carotene-linoleic	660:678	arg1	DPPH					631:634	DPPH	631:634	DPPH	631:634	The antioxidant activities were evaluated by the assay of DPPH, hydroxyl free radical, β-carotene-linoleic acid, respectfully.
27474571	3	34	theme	β-carotene-linoleic	660:678	arg1	acid					680:683	β-carotene-linoleic acid	660:683	β-carotene-linoleic acid	660:683	The antioxidant activities were evaluated by the assay of DPPH, hydroxyl free radical, β-carotene-linoleic acid, respectfully.
27474571	1	35	theme	polysaccharide	240:253	arg1	properties					195:204	physicochemical properties	179:204	physicochemical properties	179:204	The effects of ultrasonic treatment on the physicochemical properties and antioxidant activities of the polysaccharide from Cyclocarya paliurus leaves were investigated.
27474571	1	35	theme	polysaccharide	240:253	arg1	activities					222:231	antioxidant activities	210:231	antioxidant activities	210:231	The effects of ultrasonic treatment on the physicochemical properties and antioxidant activities of the polysaccharide from Cyclocarya paliurus leaves were investigated.
27474571	4	36	theme	ultrasonic	836:845	arg1	polysaccharide					847:860	the ultrasonic polysaccharide	832:860	the ultrasonic polysaccharide of Cyclocarya paliurus (UCP) by FT-IR	832:898	Results showed that no considerable change was observed on the structure between the polysaccharide of Cyclocarya paliurus (CP) and the ultrasonic polysaccharide of Cyclocarya paliurus (UCP) by FT-IR, and no apparent differences were found on the content of sugar, uronic acid and protein after the ultrasonic treatment, but difference appeared in the ratio of monosaccharide composition of CP (1.0:4.6:3.5:4.5) and UCP (1.0:5.9:3.9:4.4), and the antioxidant activities of CP were promoted after ultrasonic treatment.
27474571	1	37	dep	Cyclocarya	260:269	arg1	paliurus					271:278	Cyclocarya paliurus	260:278	Cyclocarya paliurus leaves	260:285	The effects of ultrasonic treatment on the physicochemical properties and antioxidant activities of the polysaccharide from Cyclocarya paliurus leaves were investigated.
27474571	1	37	dep	Cyclocarya	260:269	arg1	leaves					280:285	leaves	280:285	leaves	280:285	The effects of ultrasonic treatment on the physicochemical properties and antioxidant activities of the polysaccharide from Cyclocarya paliurus leaves were investigated.
27474571	3	38	theme	free	646:649	arg1	DPPH					631:634	DPPH	631:634	DPPH	631:634	The antioxidant activities were evaluated by the assay of DPPH, hydroxyl free radical, β-carotene-linoleic acid, respectfully.
27474571	3	38	theme	free	646:649	arg1	radical					651:657	hydroxyl free radical	637:657	hydroxyl free radical	637:657	The antioxidant activities were evaluated by the assay of DPPH, hydroxyl free radical, β-carotene-linoleic acid, respectfully.
27474571	0	39	theme	physicochemical	38:52	arg1	properties					54:63	physicochemical properties	38:63	physicochemical properties	38:63	Effect of ultrasonic treatment on the physicochemical properties and antioxidant activities of polysaccharide from Cyclocarya paliurus.
27474571	0	40	from	paliurus	126:133	arg1	activities					81:90	antioxidant activities	69:90	antioxidant activities	69:90	Effect of ultrasonic treatment on the physicochemical properties and antioxidant activities of polysaccharide from Cyclocarya paliurus.
27474571	0	40	from	paliurus	126:133	arg1	polysaccharide					95:108	polysaccharide	95:108	polysaccharide from Cyclocarya paliurus	95:133	Effect of ultrasonic treatment on the physicochemical properties and antioxidant activities of polysaccharide from Cyclocarya paliurus.
27474571	0	40	from	paliurus	126:133	arg1	properties					54:63	physicochemical properties	38:63	physicochemical properties	38:63	Effect of ultrasonic treatment on the physicochemical properties and antioxidant activities of polysaccharide from Cyclocarya paliurus.
27474571	1	41	dep	properties	195:204	arg1	the					175:177	the	175:177	the	175:177	The effects of ultrasonic treatment on the physicochemical properties and antioxidant activities of the polysaccharide from Cyclocarya paliurus leaves were investigated.
27474571	4	42	theme	UCP	1116:1118	arg1	composition					1076:1086	monosaccharide composition	1061:1086	monosaccharide composition of CP (1.0:4.6:3.5:4.5) and UCP (1.0:5.9:3.9:4.4)	1061:1136	Results showed that no considerable change was observed on the structure between the polysaccharide of Cyclocarya paliurus (CP) and the ultrasonic polysaccharide of Cyclocarya paliurus (UCP) by FT-IR, and no apparent differences were found on the content of sugar, uronic acid and protein after the ultrasonic treatment, but difference appeared in the ratio of monosaccharide composition of CP (1.0:4.6:3.5:4.5) and UCP (1.0:5.9:3.9:4.4), and the antioxidant activities of CP were promoted after ultrasonic treatment.
27474571	4	43	theme	paliurus	876:883	arg1	polysaccharide					785:798	the polysaccharide	781:798	the polysaccharide of Cyclocarya paliurus (CP)	781:826	Results showed that no considerable change was observed on the structure between the polysaccharide of Cyclocarya paliurus (CP) and the ultrasonic polysaccharide of Cyclocarya paliurus (UCP) by FT-IR, and no apparent differences were found on the content of sugar, uronic acid and protein after the ultrasonic treatment, but difference appeared in the ratio of monosaccharide composition of CP (1.0:4.6:3.5:4.5) and UCP (1.0:5.9:3.9:4.4), and the antioxidant activities of CP were promoted after ultrasonic treatment.
27474571	4	43	theme	paliurus	876:883	arg1	polysaccharide					847:860	the ultrasonic polysaccharide	832:860	the ultrasonic polysaccharide of Cyclocarya paliurus (UCP) by FT-IR	832:898	Results showed that no considerable change was observed on the structure between the polysaccharide of Cyclocarya paliurus (CP) and the ultrasonic polysaccharide of Cyclocarya paliurus (UCP) by FT-IR, and no apparent differences were found on the content of sugar, uronic acid and protein after the ultrasonic treatment, but difference appeared in the ratio of monosaccharide composition of CP (1.0:4.6:3.5:4.5) and UCP (1.0:5.9:3.9:4.4), and the antioxidant activities of CP were promoted after ultrasonic treatment.
27474571	4	44	theme	sugar	958:962	arg1	content					947:953	the content	943:953	the content of sugar, uronic acid and protein after the ultrasonic treatment	943:1018	Results showed that no considerable change was observed on the structure between the polysaccharide of Cyclocarya paliurus (CP) and the ultrasonic polysaccharide of Cyclocarya paliurus (UCP) by FT-IR, and no apparent differences were found on the content of sugar, uronic acid and protein after the ultrasonic treatment, but difference appeared in the ratio of monosaccharide composition of CP (1.0:4.6:3.5:4.5) and UCP (1.0:5.9:3.9:4.4), and the antioxidant activities of CP were promoted after ultrasonic treatment.
27474571	4	45	theme	Cyclocarya	865:874	arg1	paliurus					876:883	Cyclocarya paliurus	865:883	Cyclocarya paliurus (UCP) by FT-IR	865:898	Results showed that no considerable change was observed on the structure between the polysaccharide of Cyclocarya paliurus (CP) and the ultrasonic polysaccharide of Cyclocarya paliurus (UCP) by FT-IR, and no apparent differences were found on the content of sugar, uronic acid and protein after the ultrasonic treatment, but difference appeared in the ratio of monosaccharide composition of CP (1.0:4.6:3.5:4.5) and UCP (1.0:5.9:3.9:4.4), and the antioxidant activities of CP were promoted after ultrasonic treatment.
27474571	4	45	theme	Cyclocarya	865:874	arg1	UCP					886:888	UCP	886:888	UCP	886:888	Results showed that no considerable change was observed on the structure between the polysaccharide of Cyclocarya paliurus (CP) and the ultrasonic polysaccharide of Cyclocarya paliurus (UCP) by FT-IR, and no apparent differences were found on the content of sugar, uronic acid and protein after the ultrasonic treatment, but difference appeared in the ratio of monosaccharide composition of CP (1.0:4.6:3.5:4.5) and UCP (1.0:5.9:3.9:4.4), and the antioxidant activities of CP were promoted after ultrasonic treatment.
27474571	4	46	theme	Cyclocarya	803:812	arg1	CP					824:825	CP	824:825	CP	824:825	Results showed that no considerable change was observed on the structure between the polysaccharide of Cyclocarya paliurus (CP) and the ultrasonic polysaccharide of Cyclocarya paliurus (UCP) by FT-IR, and no apparent differences were found on the content of sugar, uronic acid and protein after the ultrasonic treatment, but difference appeared in the ratio of monosaccharide composition of CP (1.0:4.6:3.5:4.5) and UCP (1.0:5.9:3.9:4.4), and the antioxidant activities of CP were promoted after ultrasonic treatment.
27474571	4	46	theme	Cyclocarya	803:812	arg1	paliurus					814:821	Cyclocarya paliurus	803:821	Cyclocarya paliurus (CP)	803:826	Results showed that no considerable change was observed on the structure between the polysaccharide of Cyclocarya paliurus (CP) and the ultrasonic polysaccharide of Cyclocarya paliurus (UCP) by FT-IR, and no apparent differences were found on the content of sugar, uronic acid and protein after the ultrasonic treatment, but difference appeared in the ratio of monosaccharide composition of CP (1.0:4.6:3.5:4.5) and UCP (1.0:5.9:3.9:4.4), and the antioxidant activities of CP were promoted after ultrasonic treatment.
27474571	1	47	from	Cyclocarya	260:269	arg1	properties					195:204	physicochemical properties	179:204	physicochemical properties	179:204	The effects of ultrasonic treatment on the physicochemical properties and antioxidant activities of the polysaccharide from Cyclocarya paliurus leaves were investigated.
27474571	1	47	from	Cyclocarya	260:269	arg1	activities					222:231	antioxidant activities	210:231	antioxidant activities	210:231	The effects of ultrasonic treatment on the physicochemical properties and antioxidant activities of the polysaccharide from Cyclocarya paliurus leaves were investigated.
27474571	2	48	theme	anion	427:431	arg1	chromatography					442:455	high-performance anion exchange chromatography	410:455	high-performance anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD) and fourier transform infrared spectrometer (FT-IR) as well	410:570	The physicochemical properties were detected by high-performance gel permeation chromatography (HPGPC), high-performance anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD) and fourier transform infrared spectrometer (FT-IR) as well.
27474571	2	48	theme	anion	427:431	arg1	chromatography					386:399	high-performance gel permeation chromatography	354:399	high-performance gel permeation chromatography (HPGPC)	354:407	The physicochemical properties were detected by high-performance gel permeation chromatography (HPGPC), high-performance anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD) and fourier transform infrared spectrometer (FT-IR) as well.
27474571	4	49	located	found	934:938	arg1	content					947:953	the content	943:953	the content of sugar, uronic acid and protein after the ultrasonic treatment	943:1018	Results showed that no considerable change was observed on the structure between the polysaccharide of Cyclocarya paliurus (CP) and the ultrasonic polysaccharide of Cyclocarya paliurus (UCP) by FT-IR, and no apparent differences were found on the content of sugar, uronic acid and protein after the ultrasonic treatment, but difference appeared in the ratio of monosaccharide composition of CP (1.0:4.6:3.5:4.5) and UCP (1.0:5.9:3.9:4.4), and the antioxidant activities of CP were promoted after ultrasonic treatment.
27474571	4	49	located	found	934:938	arg2	differences					917:927	no apparent differences	905:927	no apparent differences	905:927	Results showed that no considerable change was observed on the structure between the polysaccharide of Cyclocarya paliurus (CP) and the ultrasonic polysaccharide of Cyclocarya paliurus (UCP) by FT-IR, and no apparent differences were found on the content of sugar, uronic acid and protein after the ultrasonic treatment, but difference appeared in the ratio of monosaccharide composition of CP (1.0:4.6:3.5:4.5) and UCP (1.0:5.9:3.9:4.4), and the antioxidant activities of CP were promoted after ultrasonic treatment.
27474571	0	50	theme	antioxidant	69:79	arg1	activities					81:90	antioxidant activities	69:90	antioxidant activities	69:90	Effect of ultrasonic treatment on the physicochemical properties and antioxidant activities of polysaccharide from Cyclocarya paliurus.
27474571	2	51	theme	amperometric	477:488	arg1	detection					490:498	pulsed amperometric detection	470:498	pulsed amperometric detection (HPAEC-PAD)	470:510	The physicochemical properties were detected by high-performance gel permeation chromatography (HPGPC), high-performance anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD) and fourier transform infrared spectrometer (FT-IR) as well.
27474571	2	51	theme	amperometric	477:488	arg1	HPAEC-PAD					501:509	HPAEC-PAD	501:509	HPAEC-PAD	501:509	The physicochemical properties were detected by high-performance gel permeation chromatography (HPGPC), high-performance anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD) and fourier transform infrared spectrometer (FT-IR) as well.
27474571	1	52	theme	ultrasonic	151:160	arg1	treatment					162:170	ultrasonic treatment	151:170	ultrasonic treatment	151:170	The effects of ultrasonic treatment on the physicochemical properties and antioxidant activities of the polysaccharide from Cyclocarya paliurus leaves were investigated.
27474571	4	53	theme	composition	1076:1086	arg1	ratio					1052:1056	the ratio	1048:1056	the ratio of monosaccharide composition of CP (1.0:4.6:3.5:4.5) and UCP (1.0:5.9:3.9:4.4)	1048:1136	Results showed that no considerable change was observed on the structure between the polysaccharide of Cyclocarya paliurus (CP) and the ultrasonic polysaccharide of Cyclocarya paliurus (UCP) by FT-IR, and no apparent differences were found on the content of sugar, uronic acid and protein after the ultrasonic treatment, but difference appeared in the ratio of monosaccharide composition of CP (1.0:4.6:3.5:4.5) and UCP (1.0:5.9:3.9:4.4), and the antioxidant activities of CP were promoted after ultrasonic treatment.
27474571	4	54	theme	ultrasonic	1196:1205	arg1	treatment					1207:1215	ultrasonic treatment	1196:1215	ultrasonic treatment	1196:1215	Results showed that no considerable change was observed on the structure between the polysaccharide of Cyclocarya paliurus (CP) and the ultrasonic polysaccharide of Cyclocarya paliurus (UCP) by FT-IR, and no apparent differences were found on the content of sugar, uronic acid and protein after the ultrasonic treatment, but difference appeared in the ratio of monosaccharide composition of CP (1.0:4.6:3.5:4.5) and UCP (1.0:5.9:3.9:4.4), and the antioxidant activities of CP were promoted after ultrasonic treatment.
27474571	1	55	theme	treatment	162:170	arg1	effects					140:146	The effects	136:146	The effects of ultrasonic treatment on the physicochemical properties and antioxidant activities of the polysaccharide from Cyclocarya paliurus leaves	136:285	The effects of ultrasonic treatment on the physicochemical properties and antioxidant activities of the polysaccharide from Cyclocarya paliurus leaves were investigated.
27474571	4	56	theme	protein	981:987	arg1	content					947:953	the content	943:953	the content of sugar, uronic acid and protein after the ultrasonic treatment	943:1018	Results showed that no considerable change was observed on the structure between the polysaccharide of Cyclocarya paliurus (CP) and the ultrasonic polysaccharide of Cyclocarya paliurus (UCP) by FT-IR, and no apparent differences were found on the content of sugar, uronic acid and protein after the ultrasonic treatment, but difference appeared in the ratio of monosaccharide composition of CP (1.0:4.6:3.5:4.5) and UCP (1.0:5.9:3.9:4.4), and the antioxidant activities of CP were promoted after ultrasonic treatment.
28180916	4	0	theme	B.	892:893	arg1	fibrisolvens					895:906	B. fibrisolvens	892:906	B. fibrisolvens	892:906	Results confirmed the xylan fermentation and butyrate production abilities of B. fibrisolvens.
28180916	1	1	theme	ruminants	252:260	arg1	rumens					225:230	the rumens	221:230	the rumens of cattle and other ruminants	221:260	Hemicelluloses are major components of plant biomass, but their fermentation in the rumens of cattle and other ruminants is poorly understood.
28180916	10	2	from	Differences	1693:1703	arg1	composition					1718:1728	community composition	1708:1728	community composition	1708:1728	Differences in community composition between in vitro hemicellulose enrichments and inoculum samples emerged at every phylogenetic level, suggesting that in vitro conditions provide unique selective pressures on the bacterial community and also that ruminal bacteria exhibit specialization with respect to hemicellulose utilization.
28180916	3	3	theme	cattle	752:757	arg1	contents					734:741	ruminal contents	726:741	ruminal contents of dairy cattle fed a total mixed ration (TMR) rich in hemicelluloses	726:811	We also performed Illumina sequencing of the V3/V4 region of 16S rRNA genes to determine the relative proportions of Prevotella and Butyrivibrio in hemicellulose-fed enrichment cultures inoculated from ruminal contents of dairy cattle fed a total mixed ration (TMR) rich in hemicelluloses.
28180916	10	4	theme	unique	1875:1880	arg1	pressures					1892:1900	unique selective pressures	1875:1900	unique selective pressures on the bacterial community	1875:1927	Differences in community composition between in vitro hemicellulose enrichments and inoculum samples emerged at every phylogenetic level, suggesting that in vitro conditions provide unique selective pressures on the bacterial community and also that ruminal bacteria exhibit specialization with respect to hemicellulose utilization.
28180916	4	5	theme	butyrate	859:866	arg1	production					868:877	butyrate production	859:877	butyrate production	859:877	Results confirmed the xylan fermentation and butyrate production abilities of B. fibrisolvens.
28180916	3	6	theme	Illumina	542:549	arg1	sequencing					551:560	Illumina sequencing	542:560	Illumina sequencing of the V3/V4 region of 16S rRNA genes	542:598	We also performed Illumina sequencing of the V3/V4 region of 16S rRNA genes to determine the relative proportions of Prevotella and Butyrivibrio in hemicellulose-fed enrichment cultures inoculated from ruminal contents of dairy cattle fed a total mixed ration (TMR) rich in hemicelluloses.
28180916	1	7	theme	biomass	186:192	arg1	Hemicelluloses					141:154	Hemicelluloses	141:154	Hemicelluloses	141:154	Hemicelluloses are major components of plant biomass, but their fermentation in the rumens of cattle and other ruminants is poorly understood.
28180916	1	7	theme	biomass	186:192	arg1	components					166:175	major components	160:175	major components of plant biomass	160:192	Hemicelluloses are major components of plant biomass, but their fermentation in the rumens of cattle and other ruminants is poorly understood.
28180916	9	8	from	hemicelluloses	1595:1608	arg1	differences					1523:1533	Substantial differences	1511:1533	Substantial differences in fermentation end product distribution from the different hemicelluloses	1511:1608	Substantial differences in fermentation end product distribution from the different hemicelluloses were observed, which would likely affect nutrient partitioning in the host animal.
28180916	9	8	from	hemicelluloses	1595:1608	arg1	distribution					1563:1574	fermentation end product distribution	1538:1574	fermentation end product distribution from the different hemicelluloses	1538:1608	Substantial differences in fermentation end product distribution from the different hemicelluloses were observed, which would likely affect nutrient partitioning in the host animal.
28180916	3	9	theme	mixed	771:775	arg1	TMR					785:787	TMR	785:787	TMR	785:787	We also performed Illumina sequencing of the V3/V4 region of 16S rRNA genes to determine the relative proportions of Prevotella and Butyrivibrio in hemicellulose-fed enrichment cultures inoculated from ruminal contents of dairy cattle fed a total mixed ration (TMR) rich in hemicelluloses.
28180916	3	9	theme	mixed	771:775	arg1	ration					777:782	a total mixed ration	763:782	a total mixed ration (TMR) rich in hemicelluloses	763:811	We also performed Illumina sequencing of the V3/V4 region of 16S rRNA genes to determine the relative proportions of Prevotella and Butyrivibrio in hemicellulose-fed enrichment cultures inoculated from ruminal contents of dairy cattle fed a total mixed ration (TMR) rich in hemicelluloses.
28180916	6	10	from	abundant	1111:1118	arg1	cultures					1150:1157	mixed bacterial enrichment cultures	1123:1157	mixed bacterial enrichment cultures fed the same TMR	1123:1174	Prevotella was much less abundant in mixed bacterial enrichment cultures fed the same TMR than in the ruminal inoculum, yet Prevotella was again the most abundant genus in enrichment cultures fed xylans.
28180916	0	11	theme	enrichment	120:129	arg1	cultures					131:138	ruminal enrichment cultures	112:138	ruminal enrichment cultures	112:138	Fermentation of model hemicelluloses by Prevotella strains and Butyrivibrio fibrisolvens in pure culture and in ruminal enrichment cultures.
28180916	3	12	theme	V3/V4	569:573	arg1	region					575:580	the V3/V4 region	565:580	the V3/V4 region of 16S rRNA genes	565:598	We also performed Illumina sequencing of the V3/V4 region of 16S rRNA genes to determine the relative proportions of Prevotella and Butyrivibrio in hemicellulose-fed enrichment cultures inoculated from ruminal contents of dairy cattle fed a total mixed ration (TMR) rich in hemicelluloses.
28180916	9	13	theme	end	1551:1553	arg1	product					1555:1561	fermentation end product	1538:1561	fermentation end product distribution from the different hemicelluloses	1538:1608	Substantial differences in fermentation end product distribution from the different hemicelluloses were observed, which would likely affect nutrient partitioning in the host animal.
28180916	8	14	dep	degradation	1396:1406	arg1	Butyrivibrio					1409:1420	Butyrivibrio	1409:1420	Butyrivibrio	1409:1420	Genera known for hemicellulose degradation (Butyrivibrio, Ruminococcus, and Fibrobacter) were not significantly enriched on these hemicelluloses.
28180916	8	14	dep	degradation	1396:1406	arg1	Ruminococcus					1423:1434	Ruminococcus	1423:1434	Ruminococcus	1423:1434	Genera known for hemicellulose degradation (Butyrivibrio, Ruminococcus, and Fibrobacter) were not significantly enriched on these hemicelluloses.
28180916	8	14	dep	degradation	1396:1406	arg1	Fibrobacter					1441:1451	Fibrobacter	1441:1451	Fibrobacter	1441:1451	Genera known for hemicellulose degradation (Butyrivibrio, Ruminococcus, and Fibrobacter) were not significantly enriched on these hemicelluloses.
28180916	10	15	theme	community	1708:1716	arg1	composition					1718:1728	community composition	1708:1728	community composition	1708:1728	Differences in community composition between in vitro hemicellulose enrichments and inoculum samples emerged at every phylogenetic level, suggesting that in vitro conditions provide unique selective pressures on the bacterial community and also that ruminal bacteria exhibit specialization with respect to hemicellulose utilization.
28180916	3	16	theme	rRNA	589:592	arg1	genes					594:598	16S rRNA genes	585:598	16S rRNA genes	585:598	We also performed Illumina sequencing of the V3/V4 region of 16S rRNA genes to determine the relative proportions of Prevotella and Butyrivibrio in hemicellulose-fed enrichment cultures inoculated from ruminal contents of dairy cattle fed a total mixed ration (TMR) rich in hemicelluloses.
28180916	6	17	theme	abundant	1240:1247	arg1	genus					1249:1253	the most abundant genus	1231:1253	the most abundant genus in enrichment cultures fed xylans	1231:1287	Prevotella was much less abundant in mixed bacterial enrichment cultures fed the same TMR than in the ruminal inoculum, yet Prevotella was again the most abundant genus in enrichment cultures fed xylans.
28180916	6	17	theme	abundant	1240:1247	arg1	Prevotella					1210:1219	Prevotella	1210:1219	Prevotella	1210:1219	Prevotella was much less abundant in mixed bacterial enrichment cultures fed the same TMR than in the ruminal inoculum, yet Prevotella was again the most abundant genus in enrichment cultures fed xylans.
28180916	10	18	dep	provide	1867:1873	arg1	exhibit					1960:1966	exhibit	1960:1966	exhibit specialization with respect to hemicellulose utilization	1960:2023	Differences in community composition between in vitro hemicellulose enrichments and inoculum samples emerged at every phylogenetic level, suggesting that in vitro conditions provide unique selective pressures on the bacterial community and also that ruminal bacteria exhibit specialization with respect to hemicellulose utilization.
28180916	10	19	theme	ruminal	1943:1949	arg1	bacteria					1951:1958	ruminal bacteria	1943:1958	ruminal bacteria	1943:1958	Differences in community composition between in vitro hemicellulose enrichments and inoculum samples emerged at every phylogenetic level, suggesting that in vitro conditions provide unique selective pressures on the bacterial community and also that ruminal bacteria exhibit specialization with respect to hemicellulose utilization.
28180916	1	20	theme	major	160:164	arg1	Hemicelluloses					141:154	Hemicelluloses	141:154	Hemicelluloses	141:154	Hemicelluloses are major components of plant biomass, but their fermentation in the rumens of cattle and other ruminants is poorly understood.
28180916	1	20	theme	major	160:164	arg1	components					166:175	major components	160:175	major components of plant biomass	160:192	Hemicelluloses are major components of plant biomass, but their fermentation in the rumens of cattle and other ruminants is poorly understood.
28180916	8	21	theme	hemicellulose	1382:1394	arg1	degradation					1396:1406	hemicellulose degradation	1382:1406	hemicellulose degradation (Butyrivibrio, Ruminococcus, and Fibrobacter)	1382:1452	Genera known for hemicellulose degradation (Butyrivibrio, Ruminococcus, and Fibrobacter) were not significantly enriched on these hemicelluloses.
28180916	2	22	dep	hemicelluloses	470:483	arg1	glucomannan					494:504	glucomannan	494:504	glucomannan	494:504	We compared four species of the ruminally dominant genus Prevotella and the well-known hemicellulose utilizer, Butyrivibrio fibrisolvens, with respect to degradation of several isolated hemicelluloses (xylans, glucomannan, and xyloglucan).
28180916	2	22	dep	hemicelluloses	470:483	arg1	xyloglucan					511:520	xyloglucan	511:520	xyloglucan	511:520	We compared four species of the ruminally dominant genus Prevotella and the well-known hemicellulose utilizer, Butyrivibrio fibrisolvens, with respect to degradation of several isolated hemicelluloses (xylans, glucomannan, and xyloglucan).
28180916	2	22	dep	hemicelluloses	470:483	arg1	xylans					486:491	xylans	486:491	xylans	486:491	We compared four species of the ruminally dominant genus Prevotella and the well-known hemicellulose utilizer, Butyrivibrio fibrisolvens, with respect to degradation of several isolated hemicelluloses (xylans, glucomannan, and xyloglucan).
28180916	6	23	from	genus	1249:1253	arg1	cultures					1269:1276	enrichment cultures	1258:1276	enrichment cultures fed xylans	1258:1287	Prevotella was much less abundant in mixed bacterial enrichment cultures fed the same TMR than in the ruminal inoculum, yet Prevotella was again the most abundant genus in enrichment cultures fed xylans.
28180916	9	24	theme	nutrient	1651:1658	arg1	partitioning					1660:1671	nutrient partitioning	1651:1671	nutrient partitioning	1651:1671	Substantial differences in fermentation end product distribution from the different hemicelluloses were observed, which would likely affect nutrient partitioning in the host animal.
28180916	6	25	theme	bacterial	1129:1137	arg1	cultures					1150:1157	mixed bacterial enrichment cultures	1123:1157	mixed bacterial enrichment cultures fed the same TMR	1123:1174	Prevotella was much less abundant in mixed bacterial enrichment cultures fed the same TMR than in the ruminal inoculum, yet Prevotella was again the most abundant genus in enrichment cultures fed xylans.
28180916	2	26	theme	isolated	461:468	arg1	hemicelluloses					470:483	several isolated hemicelluloses	453:483	several isolated hemicelluloses (xylans, glucomannan, and xyloglucan)	453:521	We compared four species of the ruminally dominant genus Prevotella and the well-known hemicellulose utilizer, Butyrivibrio fibrisolvens, with respect to degradation of several isolated hemicelluloses (xylans, glucomannan, and xyloglucan).
28180916	3	27	from	proportions	626:636	arg1	cultures					701:708	hemicellulose-fed enrichment cultures	672:708	hemicellulose-fed enrichment cultures inoculated from ruminal contents of dairy cattle fed a total mixed ration (TMR) rich in hemicelluloses	672:811	We also performed Illumina sequencing of the V3/V4 region of 16S rRNA genes to determine the relative proportions of Prevotella and Butyrivibrio in hemicellulose-fed enrichment cultures inoculated from ruminal contents of dairy cattle fed a total mixed ration (TMR) rich in hemicelluloses.
28180916	10	28	theme	hemicellulose	1747:1759	arg1	enrichments					1761:1771	in vitro hemicellulose enrichments	1738:1771	in vitro hemicellulose enrichments	1738:1771	Differences in community composition between in vitro hemicellulose enrichments and inoculum samples emerged at every phylogenetic level, suggesting that in vitro conditions provide unique selective pressures on the bacterial community and also that ruminal bacteria exhibit specialization with respect to hemicellulose utilization.
28180916	0	29	theme	model	16:20	arg1	hemicelluloses					22:35	model hemicelluloses	16:35	model hemicelluloses	16:35	Fermentation of model hemicelluloses by Prevotella strains and Butyrivibrio fibrisolvens in pure culture and in ruminal enrichment cultures.
28180916	9	30	theme	host	1680:1683	arg1	animal					1685:1690	the host animal	1676:1690	the host animal	1676:1690	Substantial differences in fermentation end product distribution from the different hemicelluloses were observed, which would likely affect nutrient partitioning in the host animal.
28180916	3	31	theme	Butyrivibrio	656:667	arg1	proportions					626:636	the relative proportions	613:636	the relative proportions of Prevotella and Butyrivibrio in hemicellulose-fed enrichment cultures inoculated from ruminal contents of dairy cattle fed a total mixed ration (TMR) rich in hemicelluloses	613:811	We also performed Illumina sequencing of the V3/V4 region of 16S rRNA genes to determine the relative proportions of Prevotella and Butyrivibrio in hemicellulose-fed enrichment cultures inoculated from ruminal contents of dairy cattle fed a total mixed ration (TMR) rich in hemicelluloses.
28180916	7	32	theme	Streptococcus	1347:1359	arg1	sp					1361:1362	Streptococcus sp	1347:1362	Streptococcus sp	1347:1362	By contrast, glucomannan fermentations were dominated by Streptococcus sp.
28180916	3	33	theme	Prevotella	641:650	arg1	proportions					626:636	the relative proportions	613:636	the relative proportions of Prevotella and Butyrivibrio in hemicellulose-fed enrichment cultures inoculated from ruminal contents of dairy cattle fed a total mixed ration (TMR) rich in hemicelluloses	613:811	We also performed Illumina sequencing of the V3/V4 region of 16S rRNA genes to determine the relative proportions of Prevotella and Butyrivibrio in hemicellulose-fed enrichment cultures inoculated from ruminal contents of dairy cattle fed a total mixed ration (TMR) rich in hemicelluloses.
28180916	4	34	theme	fermentation	842:853	arg1	abilities					879:887	the xylan fermentation and butyrate production abilities	832:887	the xylan fermentation and butyrate production abilities of B. fibrisolvens	832:906	Results confirmed the xylan fermentation and butyrate production abilities of B. fibrisolvens.
28180916	2	35	theme	Prevotella	341:350	arg1	species					301:307	four species	296:307	four species of the ruminally dominant genus Prevotella	296:350	We compared four species of the ruminally dominant genus Prevotella and the well-known hemicellulose utilizer, Butyrivibrio fibrisolvens, with respect to degradation of several isolated hemicelluloses (xylans, glucomannan, and xyloglucan).
28180916	2	35	theme	Prevotella	341:350	arg1	utilizer					385:392	the well-known hemicellulose utilizer	356:392	the well-known hemicellulose utilizer	356:392	We compared four species of the ruminally dominant genus Prevotella and the well-known hemicellulose utilizer, Butyrivibrio fibrisolvens, with respect to degradation of several isolated hemicelluloses (xylans, glucomannan, and xyloglucan).
28180916	2	35	theme	Prevotella	341:350	arg1	fibrisolvens					408:419	Butyrivibrio fibrisolvens	395:419	Butyrivibrio fibrisolvens	395:419	We compared four species of the ruminally dominant genus Prevotella and the well-known hemicellulose utilizer, Butyrivibrio fibrisolvens, with respect to degradation of several isolated hemicelluloses (xylans, glucomannan, and xyloglucan).
28180916	2	36	theme	dominant	326:333	arg1	Prevotella					341:350	the ruminally dominant genus Prevotella	312:350	the ruminally dominant genus Prevotella	312:350	We compared four species of the ruminally dominant genus Prevotella and the well-known hemicellulose utilizer, Butyrivibrio fibrisolvens, with respect to degradation of several isolated hemicelluloses (xylans, glucomannan, and xyloglucan).
28180916	9	37	from	differences	1523:1533	arg1	hemicelluloses					1595:1608	the different hemicelluloses	1581:1608	the different hemicelluloses	1581:1608	Substantial differences in fermentation end product distribution from the different hemicelluloses were observed, which would likely affect nutrient partitioning in the host animal.
28180916	9	37	from	differences	1523:1533	arg1	distribution					1563:1574	fermentation end product distribution	1538:1574	fermentation end product distribution from the different hemicelluloses	1538:1608	Substantial differences in fermentation end product distribution from the different hemicelluloses were observed, which would likely affect nutrient partitioning in the host animal.
28180916	3	38	theme	hemicellulose-fed	672:688	arg1	cultures					701:708	hemicellulose-fed enrichment cultures	672:708	hemicellulose-fed enrichment cultures inoculated from ruminal contents of dairy cattle fed a total mixed ration (TMR) rich in hemicelluloses	672:811	We also performed Illumina sequencing of the V3/V4 region of 16S rRNA genes to determine the relative proportions of Prevotella and Butyrivibrio in hemicellulose-fed enrichment cultures inoculated from ruminal contents of dairy cattle fed a total mixed ration (TMR) rich in hemicelluloses.
28180916	6	39	from	cultures	1150:1157	arg1	abundant					1111:1118	abundant	1111:1118	abundant	1111:1118	Prevotella was much less abundant in mixed bacterial enrichment cultures fed the same TMR than in the ruminal inoculum, yet Prevotella was again the most abundant genus in enrichment cultures fed xylans.
28180916	2	40	theme	hemicellulose	371:383	arg1	utilizer					385:392	the well-known hemicellulose utilizer	356:392	the well-known hemicellulose utilizer	356:392	We compared four species of the ruminally dominant genus Prevotella and the well-known hemicellulose utilizer, Butyrivibrio fibrisolvens, with respect to degradation of several isolated hemicelluloses (xylans, glucomannan, and xyloglucan).
28180916	2	40	theme	hemicellulose	371:383	arg1	fibrisolvens					408:419	Butyrivibrio fibrisolvens	395:419	Butyrivibrio fibrisolvens	395:419	We compared four species of the ruminally dominant genus Prevotella and the well-known hemicellulose utilizer, Butyrivibrio fibrisolvens, with respect to degradation of several isolated hemicelluloses (xylans, glucomannan, and xyloglucan).
28180916	9	41	theme	Substantial	1511:1521	arg1	differences					1523:1533	Substantial differences	1511:1533	Substantial differences in fermentation end product distribution from the different hemicelluloses	1511:1608	Substantial differences in fermentation end product distribution from the different hemicelluloses were observed, which would likely affect nutrient partitioning in the host animal.
28180916	1	42	theme	cattle	235:240	arg1	ruminants					252:260	cattle and other ruminants	235:260	cattle and other ruminants	235:260	Hemicelluloses are major components of plant biomass, but their fermentation in the rumens of cattle and other ruminants is poorly understood.
28180916	5	43	theme	fermentation	1059:1070	arg1	products					1076:1083	fermentation end products	1059:1083	fermentation end products	1059:1083	Despite their reputation as generalist fermenters, the Prevotella strains poorly fermented these hemicelluloses but exhibited dramatic differences in fermentation end products.
28180916	1	44	theme	other	246:250	arg1	ruminants					252:260	cattle and other ruminants	235:260	cattle and other ruminants	235:260	Hemicelluloses are major components of plant biomass, but their fermentation in the rumens of cattle and other ruminants is poorly understood.
28180916	10	45	dep	in	1738:1739	arg1	vitro					1741:1745	vitro	1741:1745	vitro	1741:1745	Differences in community composition between in vitro hemicellulose enrichments and inoculum samples emerged at every phylogenetic level, suggesting that in vitro conditions provide unique selective pressures on the bacterial community and also that ruminal bacteria exhibit specialization with respect to hemicellulose utilization.
28180916	10	46	theme	selective	1882:1890	arg1	pressures					1892:1900	unique selective pressures	1875:1900	unique selective pressures on the bacterial community	1875:1927	Differences in community composition between in vitro hemicellulose enrichments and inoculum samples emerged at every phylogenetic level, suggesting that in vitro conditions provide unique selective pressures on the bacterial community and also that ruminal bacteria exhibit specialization with respect to hemicellulose utilization.
28180916	10	47	theme	bacterial	1909:1917	arg1	community					1919:1927	the bacterial community	1905:1927	the bacterial community	1905:1927	Differences in community composition between in vitro hemicellulose enrichments and inoculum samples emerged at every phylogenetic level, suggesting that in vitro conditions provide unique selective pressures on the bacterial community and also that ruminal bacteria exhibit specialization with respect to hemicellulose utilization.
28180916	4	48	theme	fibrisolvens	895:906	arg1	abilities					879:887	the xylan fermentation and butyrate production abilities	832:887	the xylan fermentation and butyrate production abilities of B. fibrisolvens	832:906	Results confirmed the xylan fermentation and butyrate production abilities of B. fibrisolvens.
28180916	1	49	theme	plant	180:184	arg1	biomass					186:192	plant biomass	180:192	plant biomass	180:192	Hemicelluloses are major components of plant biomass, but their fermentation in the rumens of cattle and other ruminants is poorly understood.
28180916	3	50	theme	dairy	746:750	arg1	cattle					752:757	dairy cattle	746:757	dairy cattle	746:757	We also performed Illumina sequencing of the V3/V4 region of 16S rRNA genes to determine the relative proportions of Prevotella and Butyrivibrio in hemicellulose-fed enrichment cultures inoculated from ruminal contents of dairy cattle fed a total mixed ration (TMR) rich in hemicelluloses.
28180916	3	51	theme	region	575:580	arg1	sequencing					551:560	Illumina sequencing	542:560	Illumina sequencing of the V3/V4 region of 16S rRNA genes	542:598	We also performed Illumina sequencing of the V3/V4 region of 16S rRNA genes to determine the relative proportions of Prevotella and Butyrivibrio in hemicellulose-fed enrichment cultures inoculated from ruminal contents of dairy cattle fed a total mixed ration (TMR) rich in hemicelluloses.
28180916	5	52	from	differences	1044:1054	arg1	products					1076:1083	fermentation end products	1059:1083	fermentation end products	1059:1083	Despite their reputation as generalist fermenters, the Prevotella strains poorly fermented these hemicelluloses but exhibited dramatic differences in fermentation end products.
28180916	4	53	theme	production	868:877	arg1	abilities					879:887	the xylan fermentation and butyrate production abilities	832:887	the xylan fermentation and butyrate production abilities of B. fibrisolvens	832:906	Results confirmed the xylan fermentation and butyrate production abilities of B. fibrisolvens.
28180916	9	54	theme	fermentation	1538:1549	arg1	product					1555:1561	fermentation end product	1538:1561	fermentation end product distribution from the different hemicelluloses	1538:1608	Substantial differences in fermentation end product distribution from the different hemicelluloses were observed, which would likely affect nutrient partitioning in the host animal.
28180916	6	55	theme	ruminal	1188:1194	arg1	inoculum					1196:1203	the ruminal inoculum	1184:1203	the ruminal inoculum	1184:1203	Prevotella was much less abundant in mixed bacterial enrichment cultures fed the same TMR than in the ruminal inoculum, yet Prevotella was again the most abundant genus in enrichment cultures fed xylans.
28180916	10	56	theme	in	1738:1739	arg1	enrichments					1761:1771	in vitro hemicellulose enrichments	1738:1771	in vitro hemicellulose enrichments	1738:1771	Differences in community composition between in vitro hemicellulose enrichments and inoculum samples emerged at every phylogenetic level, suggesting that in vitro conditions provide unique selective pressures on the bacterial community and also that ruminal bacteria exhibit specialization with respect to hemicellulose utilization.
28180916	3	57	theme	total	765:769	arg1	TMR					785:787	TMR	785:787	TMR	785:787	We also performed Illumina sequencing of the V3/V4 region of 16S rRNA genes to determine the relative proportions of Prevotella and Butyrivibrio in hemicellulose-fed enrichment cultures inoculated from ruminal contents of dairy cattle fed a total mixed ration (TMR) rich in hemicelluloses.
28180916	3	57	theme	total	765:769	arg1	ration					777:782	a total mixed ration	763:782	a total mixed ration (TMR) rich in hemicelluloses	763:811	We also performed Illumina sequencing of the V3/V4 region of 16S rRNA genes to determine the relative proportions of Prevotella and Butyrivibrio in hemicellulose-fed enrichment cultures inoculated from ruminal contents of dairy cattle fed a total mixed ration (TMR) rich in hemicelluloses.
28180916	3	58	theme	genes	594:598	arg1	region					575:580	the V3/V4 region	565:580	the V3/V4 region of 16S rRNA genes	565:598	We also performed Illumina sequencing of the V3/V4 region of 16S rRNA genes to determine the relative proportions of Prevotella and Butyrivibrio in hemicellulose-fed enrichment cultures inoculated from ruminal contents of dairy cattle fed a total mixed ration (TMR) rich in hemicelluloses.
28180916	9	59	theme	product	1555:1561	arg1	distribution					1563:1574	fermentation end product distribution	1538:1574	fermentation end product distribution from the different hemicelluloses	1538:1608	Substantial differences in fermentation end product distribution from the different hemicelluloses were observed, which would likely affect nutrient partitioning in the host animal.
28180916	3	60	theme	rich	790:793	arg1	TMR					785:787	TMR	785:787	TMR	785:787	We also performed Illumina sequencing of the V3/V4 region of 16S rRNA genes to determine the relative proportions of Prevotella and Butyrivibrio in hemicellulose-fed enrichment cultures inoculated from ruminal contents of dairy cattle fed a total mixed ration (TMR) rich in hemicelluloses.
28180916	3	60	theme	rich	790:793	arg1	ration					777:782	a total mixed ration	763:782	a total mixed ration (TMR) rich in hemicelluloses	763:811	We also performed Illumina sequencing of the V3/V4 region of 16S rRNA genes to determine the relative proportions of Prevotella and Butyrivibrio in hemicellulose-fed enrichment cultures inoculated from ruminal contents of dairy cattle fed a total mixed ration (TMR) rich in hemicelluloses.
28180916	3	61	theme	16S	585:587	arg1	genes					594:598	16S rRNA genes	585:598	16S rRNA genes	585:598	We also performed Illumina sequencing of the V3/V4 region of 16S rRNA genes to determine the relative proportions of Prevotella and Butyrivibrio in hemicellulose-fed enrichment cultures inoculated from ruminal contents of dairy cattle fed a total mixed ration (TMR) rich in hemicelluloses.
28180916	5	62	theme	generalist	937:946	arg1	fermenters					948:957	generalist fermenters	937:957	generalist fermenters	937:957	Despite their reputation as generalist fermenters, the Prevotella strains poorly fermented these hemicelluloses but exhibited dramatic differences in fermentation end products.
28180916	6	63	theme	enrichment	1258:1267	arg1	cultures					1269:1276	enrichment cultures	1258:1276	enrichment cultures fed xylans	1258:1287	Prevotella was much less abundant in mixed bacterial enrichment cultures fed the same TMR than in the ruminal inoculum, yet Prevotella was again the most abundant genus in enrichment cultures fed xylans.
28180916	3	64	theme	relative	617:624	arg1	proportions					626:636	the relative proportions	613:636	the relative proportions of Prevotella and Butyrivibrio in hemicellulose-fed enrichment cultures inoculated from ruminal contents of dairy cattle fed a total mixed ration (TMR) rich in hemicelluloses	613:811	We also performed Illumina sequencing of the V3/V4 region of 16S rRNA genes to determine the relative proportions of Prevotella and Butyrivibrio in hemicellulose-fed enrichment cultures inoculated from ruminal contents of dairy cattle fed a total mixed ration (TMR) rich in hemicelluloses.
28180916	1	65	from	fermentation	205:216	arg1	rumens					225:230	the rumens	221:230	the rumens of cattle and other ruminants	221:260	Hemicelluloses are major components of plant biomass, but their fermentation in the rumens of cattle and other ruminants is poorly understood.
28180916	9	66	theme	different	1585:1593	arg1	hemicelluloses					1595:1608	the different hemicelluloses	1581:1608	the different hemicelluloses	1581:1608	Substantial differences in fermentation end product distribution from the different hemicelluloses were observed, which would likely affect nutrient partitioning in the host animal.
28180916	10	67	theme	phylogenetic	1811:1822	arg1	level					1824:1828	every phylogenetic level	1805:1828	every phylogenetic level	1805:1828	Differences in community composition between in vitro hemicellulose enrichments and inoculum samples emerged at every phylogenetic level, suggesting that in vitro conditions provide unique selective pressures on the bacterial community and also that ruminal bacteria exhibit specialization with respect to hemicellulose utilization.
28180916	0	68	theme	pure	92:95	arg1	culture					97:103	pure culture	92:103	pure culture	92:103	Fermentation of model hemicelluloses by Prevotella strains and Butyrivibrio fibrisolvens in pure culture and in ruminal enrichment cultures.
28180916	10	69	from	pressures	1892:1900	arg1	community					1919:1927	the bacterial community	1905:1927	the bacterial community	1905:1927	Differences in community composition between in vitro hemicellulose enrichments and inoculum samples emerged at every phylogenetic level, suggesting that in vitro conditions provide unique selective pressures on the bacterial community and also that ruminal bacteria exhibit specialization with respect to hemicellulose utilization.
28180916	3	70	from	rich	790:793	arg1	hemicelluloses					798:811	hemicelluloses	798:811	hemicelluloses	798:811	We also performed Illumina sequencing of the V3/V4 region of 16S rRNA genes to determine the relative proportions of Prevotella and Butyrivibrio in hemicellulose-fed enrichment cultures inoculated from ruminal contents of dairy cattle fed a total mixed ration (TMR) rich in hemicelluloses.
28180916	0	71	theme	ruminal	112:118	arg1	cultures					131:138	ruminal enrichment cultures	112:138	ruminal enrichment cultures	112:138	Fermentation of model hemicelluloses by Prevotella strains and Butyrivibrio fibrisolvens in pure culture and in ruminal enrichment cultures.
28180916	6	72	theme	enrichment	1139:1148	arg1	cultures					1150:1157	mixed bacterial enrichment cultures	1123:1157	mixed bacterial enrichment cultures fed the same TMR	1123:1174	Prevotella was much less abundant in mixed bacterial enrichment cultures fed the same TMR than in the ruminal inoculum, yet Prevotella was again the most abundant genus in enrichment cultures fed xylans.
28180916	10	73	dep	in	1847:1848	arg1	vitro					1850:1854	vitro	1850:1854	vitro	1850:1854	Differences in community composition between in vitro hemicellulose enrichments and inoculum samples emerged at every phylogenetic level, suggesting that in vitro conditions provide unique selective pressures on the bacterial community and also that ruminal bacteria exhibit specialization with respect to hemicellulose utilization.
28180916	2	74	theme	Butyrivibrio	395:406	arg1	utilizer					385:392	the well-known hemicellulose utilizer	356:392	the well-known hemicellulose utilizer	356:392	We compared four species of the ruminally dominant genus Prevotella and the well-known hemicellulose utilizer, Butyrivibrio fibrisolvens, with respect to degradation of several isolated hemicelluloses (xylans, glucomannan, and xyloglucan).
28180916	2	74	theme	Butyrivibrio	395:406	arg1	fibrisolvens					408:419	Butyrivibrio fibrisolvens	395:419	Butyrivibrio fibrisolvens	395:419	We compared four species of the ruminally dominant genus Prevotella and the well-known hemicellulose utilizer, Butyrivibrio fibrisolvens, with respect to degradation of several isolated hemicelluloses (xylans, glucomannan, and xyloglucan).
28180916	5	75	theme	Prevotella	964:973	arg1	strains					975:981	the Prevotella strains	960:981	the Prevotella strains	960:981	Despite their reputation as generalist fermenters, the Prevotella strains poorly fermented these hemicelluloses but exhibited dramatic differences in fermentation end products.
28180916	6	76	theme	mixed	1123:1127	arg1	cultures					1150:1157	mixed bacterial enrichment cultures	1123:1157	mixed bacterial enrichment cultures fed the same TMR	1123:1174	Prevotella was much less abundant in mixed bacterial enrichment cultures fed the same TMR than in the ruminal inoculum, yet Prevotella was again the most abundant genus in enrichment cultures fed xylans.
28180916	2	77	theme	several	453:459	arg1	hemicelluloses					470:483	several isolated hemicelluloses	453:483	several isolated hemicelluloses (xylans, glucomannan, and xyloglucan)	453:521	We compared four species of the ruminally dominant genus Prevotella and the well-known hemicellulose utilizer, Butyrivibrio fibrisolvens, with respect to degradation of several isolated hemicelluloses (xylans, glucomannan, and xyloglucan).
28180916	0	78	theme	hemicelluloses	22:35	arg1	Fermentation					0:11	Fermentation	0:11	Fermentation of model hemicelluloses by Prevotella strains and Butyrivibrio	0:74	Fermentation of model hemicelluloses by Prevotella strains and Butyrivibrio fibrisolvens in pure culture and in ruminal enrichment cultures.
28180916	10	79	theme	hemicellulose	1999:2011	arg1	utilization					2013:2023	hemicellulose utilization	1999:2023	hemicellulose utilization	1999:2023	Differences in community composition between in vitro hemicellulose enrichments and inoculum samples emerged at every phylogenetic level, suggesting that in vitro conditions provide unique selective pressures on the bacterial community and also that ruminal bacteria exhibit specialization with respect to hemicellulose utilization.
28180916	5	80	theme	end	1072:1074	arg1	products					1076:1083	fermentation end products	1059:1083	fermentation end products	1059:1083	Despite their reputation as generalist fermenters, the Prevotella strains poorly fermented these hemicelluloses but exhibited dramatic differences in fermentation end products.
28180916	10	81	theme	inoculum	1777:1784	arg1	samples					1786:1792	inoculum samples	1777:1792	inoculum samples	1777:1792	Differences in community composition between in vitro hemicellulose enrichments and inoculum samples emerged at every phylogenetic level, suggesting that in vitro conditions provide unique selective pressures on the bacterial community and also that ruminal bacteria exhibit specialization with respect to hemicellulose utilization.
28180916	0	82	dep	Prevotella	40:49	arg1	strains					51:57	strains	51:57	strains	51:57	Fermentation of model hemicelluloses by Prevotella strains and Butyrivibrio fibrisolvens in pure culture and in ruminal enrichment cultures.
28180916	2	83	theme	hemicelluloses	470:483	arg1	degradation					438:448	degradation	438:448	degradation of several isolated hemicelluloses (xylans, glucomannan, and xyloglucan)	438:521	We compared four species of the ruminally dominant genus Prevotella and the well-known hemicellulose utilizer, Butyrivibrio fibrisolvens, with respect to degradation of several isolated hemicelluloses (xylans, glucomannan, and xyloglucan).
28180916	3	84	from	hemicelluloses	798:811	arg1	rich					790:793	rich	790:793	rich	790:793	We also performed Illumina sequencing of the V3/V4 region of 16S rRNA genes to determine the relative proportions of Prevotella and Butyrivibrio in hemicellulose-fed enrichment cultures inoculated from ruminal contents of dairy cattle fed a total mixed ration (TMR) rich in hemicelluloses.
28180916	3	85	theme	enrichment	690:699	arg1	cultures					701:708	hemicellulose-fed enrichment cultures	672:708	hemicellulose-fed enrichment cultures inoculated from ruminal contents of dairy cattle fed a total mixed ration (TMR) rich in hemicelluloses	672:811	We also performed Illumina sequencing of the V3/V4 region of 16S rRNA genes to determine the relative proportions of Prevotella and Butyrivibrio in hemicellulose-fed enrichment cultures inoculated from ruminal contents of dairy cattle fed a total mixed ration (TMR) rich in hemicelluloses.
28180916	4	86	theme	xylan	836:840	arg1	fermentation					842:853	xylan fermentation	836:853	xylan fermentation	836:853	Results confirmed the xylan fermentation and butyrate production abilities of B. fibrisolvens.
28180916	2	87	theme	genus	335:339	arg1	Prevotella					341:350	the ruminally dominant genus Prevotella	312:350	the ruminally dominant genus Prevotella	312:350	We compared four species of the ruminally dominant genus Prevotella and the well-known hemicellulose utilizer, Butyrivibrio fibrisolvens, with respect to degradation of several isolated hemicelluloses (xylans, glucomannan, and xyloglucan).
28180916	5	88	theme	dramatic	1035:1042	arg1	differences					1044:1054	dramatic differences	1035:1054	dramatic differences in fermentation end products	1035:1083	Despite their reputation as generalist fermenters, the Prevotella strains poorly fermented these hemicelluloses but exhibited dramatic differences in fermentation end products.
28180916	7	89	theme	glucomannan	1303:1313	arg1	fermentations					1315:1327	glucomannan fermentations	1303:1327	glucomannan fermentations	1303:1327	By contrast, glucomannan fermentations were dominated by Streptococcus sp.
28180916	2	90	theme	well-known	360:369	arg1	utilizer					385:392	the well-known hemicellulose utilizer	356:392	the well-known hemicellulose utilizer	356:392	We compared four species of the ruminally dominant genus Prevotella and the well-known hemicellulose utilizer, Butyrivibrio fibrisolvens, with respect to degradation of several isolated hemicelluloses (xylans, glucomannan, and xyloglucan).
28180916	2	90	theme	well-known	360:369	arg1	fibrisolvens					408:419	Butyrivibrio fibrisolvens	395:419	Butyrivibrio fibrisolvens	395:419	We compared four species of the ruminally dominant genus Prevotella and the well-known hemicellulose utilizer, Butyrivibrio fibrisolvens, with respect to degradation of several isolated hemicelluloses (xylans, glucomannan, and xyloglucan).
28180916	6	91	theme	same	1167:1170	arg1	TMR					1172:1174	the same TMR	1163:1174	the same TMR	1163:1174	Prevotella was much less abundant in mixed bacterial enrichment cultures fed the same TMR than in the ruminal inoculum, yet Prevotella was again the most abundant genus in enrichment cultures fed xylans.
28180916	3	92	theme	ruminal	726:732	arg1	contents					734:741	ruminal contents	726:741	ruminal contents of dairy cattle fed a total mixed ration (TMR) rich in hemicelluloses	726:811	We also performed Illumina sequencing of the V3/V4 region of 16S rRNA genes to determine the relative proportions of Prevotella and Butyrivibrio in hemicellulose-fed enrichment cultures inoculated from ruminal contents of dairy cattle fed a total mixed ration (TMR) rich in hemicelluloses.
28180916	10	93	theme	in	1847:1848	arg1	conditions					1856:1865	in vitro conditions	1847:1865	in vitro conditions	1847:1865	Differences in community composition between in vitro hemicellulose enrichments and inoculum samples emerged at every phylogenetic level, suggesting that in vitro conditions provide unique selective pressures on the bacterial community and also that ruminal bacteria exhibit specialization with respect to hemicellulose utilization.
28891606	0	0	theme	components	75:84	arg1	metabolism					86:95	components metabolism	75:95	components metabolism	75:95	[Effects of endophytic fungi from Dendrobium officinale on host growth and components metabolism of tissue culture seedlings].
28891606	4	1	theme	alcohol-soluble	633:647	arg1	extracts					649:656	alcohol-soluble extracts	633:656	alcohol-soluble extracts	633:656	Monosacchride composition of polysaccharides and alcohol-soluble extracts components were analyzed by pre-column derivatives HPLC and HPLC method, respectively.
28891606	1	2	from	effects	155:161	arg1	metabolism					254:263	growth and components metabolism	232:263	growth and components metabolism of tissue culture seedlings	232:291	The paper aims to study the effects of endophytic fungi from D. officinale cultivated on living trees on growth and components metabolism of tissue culture seedlings.
28891606	1	2	from	effects	155:161	arg1	officinale					191:200	D. officinale	188:200	D. officinale	188:200	The paper aims to study the effects of endophytic fungi from D. officinale cultivated on living trees on growth and components metabolism of tissue culture seedlings.
28891606	2	3	theme	culture	359:365	arg1	seedlings					367:375	tissue culture seedlings	352:375	tissue culture seedlings infected and uninfected	352:399	Morphological characteristics and agronomic characters of tissue culture seedlings infected and uninfected by endophytic fungus were observed and measured.
28891606	10	4	theme	mechanism	1282:1290	arg1	research					1292:1299	mechanism research	1282:1299	mechanism research between endophytic fungus and their hosts and microbiology engineering	1282:1370	On the basis, four superior strains were selected for mechanism research between endophytic fungus and their hosts and microbiology engineering.
28891606	2	5	theme	tissue	352:357	arg1	seedlings					367:375	tissue culture seedlings	352:375	tissue culture seedlings infected and uninfected	352:399	Morphological characteristics and agronomic characters of tissue culture seedlings infected and uninfected by endophytic fungus were observed and measured.
28891606	1	6	theme	growth	232:237	arg1	metabolism					254:263	growth and components metabolism	232:263	growth and components metabolism of tissue culture seedlings	232:291	The paper aims to study the effects of endophytic fungi from D. officinale cultivated on living trees on growth and components metabolism of tissue culture seedlings.
28891606	5	7	theme	nodes	797:801	arg1	purple					782:787	purple	782:787	purple of stem nodes	782:801	It showed that effects of turning to purple of stem nodes could be changed by endophytic fungus.
28891606	7	8	theme	tissue	1051:1056	arg1	seedlings					1066:1074	tissue culture seedlings	1051:1074	tissue culture seedlings	1051:1074	The strains tested, expect DO34, could promote growth and polysaccharides content of tissue culture seedlings.
28891606	7	9	theme	seedlings	1066:1074	arg1	growth					1013:1018	growth	1013:1018	growth	1013:1018	The strains tested, expect DO34, could promote growth and polysaccharides content of tissue culture seedlings.
28891606	7	9	theme	seedlings	1066:1074	arg1	content					1040:1046	polysaccharides content	1024:1046	polysaccharides content	1024:1046	The strains tested, expect DO34, could promote growth and polysaccharides content of tissue culture seedlings.
28891606	7	10	theme	polysaccharides	1024:1038	arg1	content					1040:1046	polysaccharides content	1024:1046	polysaccharides content	1024:1046	The strains tested, expect DO34, could promote growth and polysaccharides content of tissue culture seedlings.
28891606	1	11	theme	components	243:252	arg1	metabolism					254:263	growth and components metabolism	232:263	growth and components metabolism of tissue culture seedlings	232:291	The paper aims to study the effects of endophytic fungi from D. officinale cultivated on living trees on growth and components metabolism of tissue culture seedlings.
28891606	0	12	theme	culture	107:113	arg1	seedlings					115:123	tissue culture seedlings	100:123	tissue culture seedlings	100:123	[Effects of endophytic fungi from Dendrobium officinale on host growth and components metabolism of tissue culture seedlings].
28891606	0	13	from	officinale	45:54	arg1	[Effects					0:7	[Effects	0:7	[Effects of endophytic fungi from Dendrobium officinale on host growth and components metabolism of tissue culture seedlings	0:123	[Effects of endophytic fungi from Dendrobium officinale on host growth and components metabolism of tissue culture seedlings].
28891606	0	13	from	officinale	45:54	arg1	growth					64:69	host growth	59:69	host growth	59:69	[Effects of endophytic fungi from Dendrobium officinale on host growth and components metabolism of tissue culture seedlings].
28891606	0	13	from	officinale	45:54	arg1	metabolism					86:95	components metabolism	75:95	components metabolism	75:95	[Effects of endophytic fungi from Dendrobium officinale on host growth and components metabolism of tissue culture seedlings].
28891606	2	14	theme	endophytic	404:413	arg1	fungus					415:420	endophytic fungus	404:420	endophytic fungus	404:420	Morphological characteristics and agronomic characters of tissue culture seedlings infected and uninfected by endophytic fungus were observed and measured.
28891606	0	15	theme	tissue	100:105	arg1	seedlings					115:123	tissue culture seedlings	100:123	tissue culture seedlings	100:123	[Effects of endophytic fungi from Dendrobium officinale on host growth and components metabolism of tissue culture seedlings].
28891606	4	16	dep	polysaccharides	613:627	arg1	components					658:667	components	658:667	components	658:667	Monosacchride composition of polysaccharides and alcohol-soluble extracts components were analyzed by pre-column derivatives HPLC and HPLC method, respectively.
28891606	6	17	theme	polysaccharides	920:934	arg1	constitutions					903:915	constitutions	903:915	constitutions	903:915	Besides, the endophytic fungus could affect the contents and constitutions of polysaccharides and alcohol-soluble extracts.
28891606	6	17	theme	polysaccharides	920:934	arg1	contents					890:897	contents	890:897	contents	890:897	Besides, the endophytic fungus could affect the contents and constitutions of polysaccharides and alcohol-soluble extracts.
28891606	8	18	theme	mannose	1144:1150	arg1	accumulation					1128:1139	the accumulation	1124:1139	the accumulation of mannose	1124:1150	The strains tested, expect DO12, could promote the accumulation of mannose.
28891606	10	19	theme	microbiology	1347:1358	arg1	engineering					1360:1370	microbiology engineering	1347:1370	microbiology engineering	1347:1370	On the basis, four superior strains were selected for mechanism research between endophytic fungus and their hosts and microbiology engineering.
28891606	2	20	theme	uninfected	390:399	arg1	seedlings					367:375	tissue culture seedlings	352:375	tissue culture seedlings infected and uninfected	352:399	Morphological characteristics and agronomic characters of tissue culture seedlings infected and uninfected by endophytic fungus were observed and measured.
28891606	4	21	theme	polysaccharides	613:627	arg1	composition					598:608	Monosacchride composition	584:608	Monosacchride composition of polysaccharides and alcohol-soluble extracts components	584:667	Monosacchride composition of polysaccharides and alcohol-soluble extracts components were analyzed by pre-column derivatives HPLC and HPLC method, respectively.
28891606	2	22	theme	Morphological	294:306	arg1	characteristics					308:322	Morphological characteristics	294:322	Morphological characteristics	294:322	Morphological characteristics and agronomic characters of tissue culture seedlings infected and uninfected by endophytic fungus were observed and measured.
28891606	6	23	theme	endophytic	855:864	arg1	fungus					866:871	the endophytic fungus	851:871	the endophytic fungus	851:871	Besides, the endophytic fungus could affect the contents and constitutions of polysaccharides and alcohol-soluble extracts.
28891606	1	24	theme	endophytic	166:175	arg1	fungi					177:181	endophytic fungi	166:181	endophytic fungi from D. officinale cultivated on living trees	166:227	The paper aims to study the effects of endophytic fungi from D. officinale cultivated on living trees on growth and components metabolism of tissue culture seedlings.
28891606	0	25	theme	fungi	23:27	arg1	[Effects					0:7	[Effects	0:7	[Effects of endophytic fungi from Dendrobium officinale on host growth and components metabolism of tissue culture seedlings	0:123	[Effects of endophytic fungi from Dendrobium officinale on host growth and components metabolism of tissue culture seedlings].
28891606	6	26	dep	contents	890:897	arg1	the					886:888	the	886:888	the	886:888	Besides, the endophytic fungus could affect the contents and constitutions of polysaccharides and alcohol-soluble extracts.
28891606	1	27	theme	fungi	177:181	arg1	effects					155:161	the effects	151:161	the effects of endophytic fungi from D. officinale cultivated on living trees on growth and components metabolism of tissue culture seedlings	151:291	The paper aims to study the effects of endophytic fungi from D. officinale cultivated on living trees on growth and components metabolism of tissue culture seedlings.
28891606	0	28	theme	endophytic	12:21	arg1	fungi					23:27	endophytic fungi	12:27	endophytic fungi	12:27	[Effects of endophytic fungi from Dendrobium officinale on host growth and components metabolism of tissue culture seedlings].
28891606	1	29	theme	tissue	268:273	arg1	seedlings					283:291	tissue culture seedlings	268:291	tissue culture seedlings	268:291	The paper aims to study the effects of endophytic fungi from D. officinale cultivated on living trees on growth and components metabolism of tissue culture seedlings.
28891606	0	30	theme	seedlings	115:123	arg1	growth					64:69	host growth	59:69	host growth	59:69	[Effects of endophytic fungi from Dendrobium officinale on host growth and components metabolism of tissue culture seedlings].
28891606	0	30	theme	seedlings	115:123	arg1	metabolism					86:95	components metabolism	75:95	components metabolism	75:95	[Effects of endophytic fungi from Dendrobium officinale on host growth and components metabolism of tissue culture seedlings].
28891606	6	31	theme	extracts	956:963	arg1	constitutions					903:915	constitutions	903:915	constitutions	903:915	Besides, the endophytic fungus could affect the contents and constitutions of polysaccharides and alcohol-soluble extracts.
28891606	6	31	theme	extracts	956:963	arg1	contents					890:897	contents	890:897	contents	890:897	Besides, the endophytic fungus could affect the contents and constitutions of polysaccharides and alcohol-soluble extracts.
28891606	0	32	theme	Dendrobium	34:43	arg1	officinale					45:54	Dendrobium officinale	34:54	Dendrobium officinale on host growth and components metabolism of tissue culture seedlings	34:123	[Effects of endophytic fungi from Dendrobium officinale on host growth and components metabolism of tissue culture seedlings].
28891606	1	33	theme	culture	275:281	arg1	seedlings					283:291	tissue culture seedlings	268:291	tissue culture seedlings	268:291	The paper aims to study the effects of endophytic fungi from D. officinale cultivated on living trees on growth and components metabolism of tissue culture seedlings.
28891606	3	34	theme	alcohol-soluble	470:484	arg1	extracts					486:493	alcohol-soluble extracts	470:493	alcohol-soluble extracts	470:493	Polysaccharides and alcohol-soluble extracts contents were determined by phenol-sulfuric acid method and hot-dipmethod, respectively.
28891606	1	35	theme	D.	188:189	arg1	officinale					191:200	D. officinale	188:200	D. officinale	188:200	The paper aims to study the effects of endophytic fungi from D. officinale cultivated on living trees on growth and components metabolism of tissue culture seedlings.
28891606	3	36	theme	Polysaccharides	450:464	arg1	contents					495:502	Polysaccharides and alcohol-soluble extracts contents	450:502	Polysaccharides and alcohol-soluble extracts contents	450:502	Polysaccharides and alcohol-soluble extracts contents were determined by phenol-sulfuric acid method and hot-dipmethod, respectively.
28891606	1	37	theme	seedlings	283:291	arg1	metabolism					254:263	growth and components metabolism	232:263	growth and components metabolism of tissue culture seedlings	232:291	The paper aims to study the effects of endophytic fungi from D. officinale cultivated on living trees on growth and components metabolism of tissue culture seedlings.
28891606	4	38	theme	extracts	649:656	arg1	composition					598:608	Monosacchride composition	584:608	Monosacchride composition of polysaccharides and alcohol-soluble extracts components	584:667	Monosacchride composition of polysaccharides and alcohol-soluble extracts components were analyzed by pre-column derivatives HPLC and HPLC method, respectively.
28891606	8	39	dep	DO12	1104:1107	arg1	expect					1097:1102	expect	1097:1102	expect	1097:1102	The strains tested, expect DO12, could promote the accumulation of mannose.
28891606	3	40	theme	extracts	486:493	arg1	contents					495:502	Polysaccharides and alcohol-soluble extracts contents	450:502	Polysaccharides and alcohol-soluble extracts contents	450:502	Polysaccharides and alcohol-soluble extracts contents were determined by phenol-sulfuric acid method and hot-dipmethod, respectively.
28891606	5	41	theme	endophytic	823:832	arg1	fungus					834:839	endophytic fungus	823:839	endophytic fungus	823:839	It showed that effects of turning to purple of stem nodes could be changed by endophytic fungus.
28891606	1	42	from	officinale	191:200	arg1	effects					155:161	the effects	151:161	the effects of endophytic fungi from D. officinale cultivated on living trees on growth and components metabolism of tissue culture seedlings	151:291	The paper aims to study the effects of endophytic fungi from D. officinale cultivated on living trees on growth and components metabolism of tissue culture seedlings.
28891606	1	42	from	officinale	191:200	arg1	fungi					177:181	endophytic fungi	166:181	endophytic fungi from D. officinale cultivated on living trees	166:227	The paper aims to study the effects of endophytic fungi from D. officinale cultivated on living trees on growth and components metabolism of tissue culture seedlings.
28891606	3	43	theme	phenol-sulfuric	523:537	arg1	method					544:549	phenol-sulfuric acid method	523:549	phenol-sulfuric acid method	523:549	Polysaccharides and alcohol-soluble extracts contents were determined by phenol-sulfuric acid method and hot-dipmethod, respectively.
28891606	5	44	theme	stem	792:795	arg1	nodes					797:801	stem nodes	792:801	stem nodes	792:801	It showed that effects of turning to purple of stem nodes could be changed by endophytic fungus.
28891606	9	45	theme	alcohol-soluble	1202:1216	arg1	extracts					1218:1225	alcohol-soluble extracts	1202:1225	alcohol-soluble extracts	1202:1225	Furthermore, DO18, DO19 and DO120 could increase alcohol-soluble extracts.
28891606	3	46	theme	acid	539:542	arg1	method					544:549	phenol-sulfuric acid method	523:549	phenol-sulfuric acid method	523:549	Polysaccharides and alcohol-soluble extracts contents were determined by phenol-sulfuric acid method and hot-dipmethod, respectively.
28891606	4	47	theme	Monosacchride	584:596	arg1	composition					598:608	Monosacchride composition	584:608	Monosacchride composition of polysaccharides and alcohol-soluble extracts components	584:667	Monosacchride composition of polysaccharides and alcohol-soluble extracts components were analyzed by pre-column derivatives HPLC and HPLC method, respectively.
28891606	4	48	dep	derivatives	697:707	arg1	method					723:728	HPLC method	718:728	HPLC method	718:728	Monosacchride composition of polysaccharides and alcohol-soluble extracts components were analyzed by pre-column derivatives HPLC and HPLC method, respectively.
28891606	4	48	dep	derivatives	697:707	arg1	derivatives					697:707	pre-column derivatives HPLC and HPLC method	686:728	pre-column derivatives HPLC and HPLC method	686:728	Monosacchride composition of polysaccharides and alcohol-soluble extracts components were analyzed by pre-column derivatives HPLC and HPLC method, respectively.
28891606	4	48	dep	derivatives	697:707	arg1	HPLC					709:712	HPLC	709:712	HPLC	709:712	Monosacchride composition of polysaccharides and alcohol-soluble extracts components were analyzed by pre-column derivatives HPLC and HPLC method, respectively.
28891606	7	49	dep	expect	986:991	arg1	promote					1005:1011	promote	1005:1011	could promote growth and polysaccharides content of tissue culture seedlings	999:1074	The strains tested, expect DO34, could promote growth and polysaccharides content of tissue culture seedlings.
28891606	2	50	theme	agronomic	328:336	arg1	characters					338:347	agronomic characters	328:347	agronomic characters	328:347	Morphological characteristics and agronomic characters of tissue culture seedlings infected and uninfected by endophytic fungus were observed and measured.
28891606	10	51	theme	endophytic	1309:1318	arg1	fungus					1320:1325	endophytic fungus	1309:1325	endophytic fungus	1309:1325	On the basis, four superior strains were selected for mechanism research between endophytic fungus and their hosts and microbiology engineering.
28891606	4	52	theme	pre-column	686:695	arg1	method					723:728	HPLC method	718:728	HPLC method	718:728	Monosacchride composition of polysaccharides and alcohol-soluble extracts components were analyzed by pre-column derivatives HPLC and HPLC method, respectively.
28891606	4	52	theme	pre-column	686:695	arg1	derivatives					697:707	pre-column derivatives HPLC and HPLC method	686:728	pre-column derivatives HPLC and HPLC method	686:728	Monosacchride composition of polysaccharides and alcohol-soluble extracts components were analyzed by pre-column derivatives HPLC and HPLC method, respectively.
28891606	4	52	theme	pre-column	686:695	arg1	HPLC					709:712	HPLC	709:712	HPLC	709:712	Monosacchride composition of polysaccharides and alcohol-soluble extracts components were analyzed by pre-column derivatives HPLC and HPLC method, respectively.
28891606	5	53	theme	turning	771:777	arg1	effects					760:766	effects	760:766	effects of turning to purple of stem nodes	760:801	It showed that effects of turning to purple of stem nodes could be changed by endophytic fungus.
28891606	10	54	theme	superior	1247:1254	arg1	strains					1256:1262	four superior strains	1242:1262	four superior strains	1242:1262	On the basis, four superior strains were selected for mechanism research between endophytic fungus and their hosts and microbiology engineering.
28891606	6	55	theme	alcohol-soluble	940:954	arg1	extracts					956:963	alcohol-soluble extracts	940:963	alcohol-soluble extracts	940:963	Besides, the endophytic fungus could affect the contents and constitutions of polysaccharides and alcohol-soluble extracts.
28891606	4	56	theme	HPLC	718:721	arg1	method					723:728	HPLC method	718:728	HPLC method	718:728	Monosacchride composition of polysaccharides and alcohol-soluble extracts components were analyzed by pre-column derivatives HPLC and HPLC method, respectively.
28891606	4	56	theme	HPLC	718:721	arg1	derivatives					697:707	pre-column derivatives HPLC and HPLC method	686:728	pre-column derivatives HPLC and HPLC method	686:728	Monosacchride composition of polysaccharides and alcohol-soluble extracts components were analyzed by pre-column derivatives HPLC and HPLC method, respectively.
28891606	2	57	theme	infected	377:384	arg1	seedlings					367:375	tissue culture seedlings	352:375	tissue culture seedlings infected and uninfected	352:399	Morphological characteristics and agronomic characters of tissue culture seedlings infected and uninfected by endophytic fungus were observed and measured.
28891606	1	58	theme	living	216:221	arg1	trees					223:227	living trees	216:227	living trees	216:227	The paper aims to study the effects of endophytic fungi from D. officinale cultivated on living trees on growth and components metabolism of tissue culture seedlings.
28891606	0	59	theme	host	59:62	arg1	growth					64:69	host growth	59:69	host growth	59:69	[Effects of endophytic fungi from Dendrobium officinale on host growth and components metabolism of tissue culture seedlings].
28891606	7	60	theme	culture	1058:1064	arg1	seedlings					1066:1074	tissue culture seedlings	1051:1074	tissue culture seedlings	1051:1074	The strains tested, expect DO34, could promote growth and polysaccharides content of tissue culture seedlings.
28891606	2	61	theme	seedlings	367:375	arg1	characters					338:347	agronomic characters	328:347	agronomic characters	328:347	Morphological characteristics and agronomic characters of tissue culture seedlings infected and uninfected by endophytic fungus were observed and measured.
28891606	2	61	theme	seedlings	367:375	arg1	characteristics					308:322	Morphological characteristics	294:322	Morphological characteristics	294:322	Morphological characteristics and agronomic characters of tissue culture seedlings infected and uninfected by endophytic fungus were observed and measured.
24607166	4	0	theme	composite	822:830	arg1	properties					804:813	static and dynamic mechanical properties	774:813	static and dynamic mechanical properties	774:813	We also analyzed the mechanism of the water-triggered shape-memory effect by considering the microstructure, water contact angle, water uptake, thermal properties and static and dynamic mechanical properties of the composite.
24607166	4	0	theme	composite	822:830	arg1	microstructure					700:713	the microstructure	696:713	the microstructure	696:713	We also analyzed the mechanism of the water-triggered shape-memory effect by considering the microstructure, water contact angle, water uptake, thermal properties and static and dynamic mechanical properties of the composite.
24607166	4	0	theme	composite	822:830	arg1	properties					759:768	thermal properties	751:768	thermal properties	751:768	We also analyzed the mechanism of the water-triggered shape-memory effect by considering the microstructure, water contact angle, water uptake, thermal properties and static and dynamic mechanical properties of the composite.
24607166	2	1	theme	water-induced	351:363	arg1	composites					378:387	water-induced shape-memory composites	351:387	water-induced shape-memory composites composed of microcrystalline cellulose (MCC) and poly(d,l-lactide) (PDLLA)	351:462	In this study, we prepared water-induced shape-memory composites composed of microcrystalline cellulose (MCC) and poly(d,l-lactide) (PDLLA).
24607166	5	2	theme	in	848:849	arg1	analysis					869:876	in vitro degradation analysis	848:876	in vitro degradation analysis	848:876	The results of in vitro degradation analysis demonstrate that the composites exhibited good biodegradation.
24607166	6	3	theme	blue	961:964	arg1	assays					966:971	Alamar blue assays	954:971	Alamar blue assays based on osteoblasts	954:992	In addition, Alamar blue assays based on osteoblasts indicate that the composites possess good cytocompatibility.
24607166	4	4	theme	water	737:741	arg1	microstructure					700:713	the microstructure	696:713	the microstructure	696:713	We also analyzed the mechanism of the water-triggered shape-memory effect by considering the microstructure, water contact angle, water uptake, thermal properties and static and dynamic mechanical properties of the composite.
24607166	4	4	theme	water	737:741	arg1	uptake					743:748	water uptake	737:748	water uptake	737:748	We also analyzed the mechanism of the water-triggered shape-memory effect by considering the microstructure, water contact angle, water uptake, thermal properties and static and dynamic mechanical properties of the composite.
24607166	3	5	with	composite	486:494	arg1	content					508:514	an MCC content	501:514	an MCC content of 35% (PDLLA-MCC-35)	501:536	We observed that the composite with an MCC content of 35% (PDLLA-MCC-35) exhibited a good shape-memory effect upon exposure to water at 37°C.
24607166	4	6	theme	contact	722:728	arg1	microstructure					700:713	the microstructure	696:713	the microstructure	696:713	We also analyzed the mechanism of the water-triggered shape-memory effect by considering the microstructure, water contact angle, water uptake, thermal properties and static and dynamic mechanical properties of the composite.
24607166	4	6	theme	contact	722:728	arg1	angle					730:734	water contact angle	716:734	water contact angle	716:734	We also analyzed the mechanism of the water-triggered shape-memory effect by considering the microstructure, water contact angle, water uptake, thermal properties and static and dynamic mechanical properties of the composite.
24607166	5	7	theme	good	920:923	arg1	biodegradation					925:938	good biodegradation	920:938	good biodegradation	920:938	The results of in vitro degradation analysis demonstrate that the composites exhibited good biodegradation.
24607166	6	8	theme	Alamar	954:959	arg1	assays					966:971	Alamar blue assays	954:971	Alamar blue assays based on osteoblasts	954:992	In addition, Alamar blue assays based on osteoblasts indicate that the composites possess good cytocompatibility.
24607166	5	9	dep	in	848:849	arg1	vitro					851:855	vitro	851:855	vitro	851:855	The results of in vitro degradation analysis demonstrate that the composites exhibited good biodegradation.
24607166	4	10	theme	water	716:720	arg1	microstructure					700:713	the microstructure	696:713	the microstructure	696:713	We also analyzed the mechanism of the water-triggered shape-memory effect by considering the microstructure, water contact angle, water uptake, thermal properties and static and dynamic mechanical properties of the composite.
24607166	4	10	theme	water	716:720	arg1	angle					730:734	water contact angle	716:734	water contact angle	716:734	We also analyzed the mechanism of the water-triggered shape-memory effect by considering the microstructure, water contact angle, water uptake, thermal properties and static and dynamic mechanical properties of the composite.
24607166	5	11	theme	degradation	857:867	arg1	analysis					869:876	in vitro degradation analysis	848:876	in vitro degradation analysis	848:876	The results of in vitro degradation analysis demonstrate that the composites exhibited good biodegradation.
24607166	4	12	theme	mechanical	793:802	arg1	properties					804:813	static and dynamic mechanical properties	774:813	static and dynamic mechanical properties	774:813	We also analyzed the mechanism of the water-triggered shape-memory effect by considering the microstructure, water contact angle, water uptake, thermal properties and static and dynamic mechanical properties of the composite.
24607166	4	12	theme	mechanical	793:802	arg1	microstructure					700:713	the microstructure	696:713	the microstructure	696:713	We also analyzed the mechanism of the water-triggered shape-memory effect by considering the microstructure, water contact angle, water uptake, thermal properties and static and dynamic mechanical properties of the composite.
24607166	7	13	theme	water-induced	1070:1082	arg1	composite					1097:1105	the water-induced shape-memory composite	1066:1105	the water-induced shape-memory composite	1066:1105	Therefore, the water-induced shape-memory composite can potentially be developed into a new smart medical device.
24607166	3	14	from	37°C	601:604	arg1	water					592:596	water	592:596	water at 37°C	592:604	We observed that the composite with an MCC content of 35% (PDLLA-MCC-35) exhibited a good shape-memory effect upon exposure to water at 37°C.
24607166	2	15	dep	poly	438:441	arg1	d					443:443	d	443:443	d	443:443	In this study, we prepared water-induced shape-memory composites composed of microcrystalline cellulose (MCC) and poly(d,l-lactide) (PDLLA).
24607166	2	15	dep	poly	438:441	arg1	l-lactide					445:453	l-lactide	445:453	l-lactide	445:453	In this study, we prepared water-induced shape-memory composites composed of microcrystalline cellulose (MCC) and poly(d,l-lactide) (PDLLA).
24607166	3	16	theme	MCC	504:506	arg1	content					508:514	an MCC content	501:514	an MCC content of 35% (PDLLA-MCC-35)	501:536	We observed that the composite with an MCC content of 35% (PDLLA-MCC-35) exhibited a good shape-memory effect upon exposure to water at 37°C.
24607166	1	17	theme	biomedical	169:178	arg1	applications					180:191	their biomedical applications	163:191	their biomedical applications	163:191	Thermo-induced shape-memory polymers (SMPs) have been widely investigated, but their biomedical applications are limited because their switching temperature does not fall completely within the range of room temperature to body temperature.
24607166	0	18	theme	shape-memory	14:25	arg1	poly					27:30	Water-induced shape-memory poly	0:30	Water-induced shape-memory poly(D,L-lactide)	0:43	Water-induced shape-memory poly(D,L-lactide)/microcrystalline cellulose composites.
24607166	6	19	contain	possess	1023:1029	arg1	composites					1012:1021	the composites	1008:1021	the composites	1008:1021	In addition, Alamar blue assays based on osteoblasts indicate that the composites possess good cytocompatibility.
24607166	6	19	contain	possess	1023:1029	arg2	cytocompatibility					1036:1052	good cytocompatibility	1031:1052	good cytocompatibility	1031:1052	In addition, Alamar blue assays based on osteoblasts indicate that the composites possess good cytocompatibility.
24607166	1	20	theme	Thermo-induced	84:97	arg1	SMPs					122:125	SMPs	122:125	SMPs	122:125	Thermo-induced shape-memory polymers (SMPs) have been widely investigated, but their biomedical applications are limited because their switching temperature does not fall completely within the range of room temperature to body temperature.
24607166	1	20	theme	Thermo-induced	84:97	arg1	polymers					112:119	Thermo-induced shape-memory polymers	84:119	Thermo-induced shape-memory polymers (SMPs)	84:126	Thermo-induced shape-memory polymers (SMPs) have been widely investigated, but their biomedical applications are limited because their switching temperature does not fall completely within the range of room temperature to body temperature.
24607166	0	21	theme	Water-induced	0:12	arg1	poly					27:30	Water-induced shape-memory poly	0:30	Water-induced shape-memory poly(D,L-lactide)	0:43	Water-induced shape-memory poly(D,L-lactide)/microcrystalline cellulose composites.
24607166	1	22	theme	shape-memory	99:110	arg1	SMPs					122:125	SMPs	122:125	SMPs	122:125	Thermo-induced shape-memory polymers (SMPs) have been widely investigated, but their biomedical applications are limited because their switching temperature does not fall completely within the range of room temperature to body temperature.
24607166	1	22	theme	shape-memory	99:110	arg1	polymers					112:119	Thermo-induced shape-memory polymers	84:119	Thermo-induced shape-memory polymers (SMPs)	84:126	Thermo-induced shape-memory polymers (SMPs) have been widely investigated, but their biomedical applications are limited because their switching temperature does not fall completely within the range of room temperature to body temperature.
24607166	6	23	theme	good	1031:1034	arg1	cytocompatibility					1036:1052	good cytocompatibility	1031:1052	good cytocompatibility	1031:1052	In addition, Alamar blue assays based on osteoblasts indicate that the composites possess good cytocompatibility.
24607166	7	24	theme	smart	1147:1151	arg1	device					1161:1166	a new smart medical device	1141:1166	a new smart medical device	1141:1166	Therefore, the water-induced shape-memory composite can potentially be developed into a new smart medical device.
24607166	1	25	theme	room	286:289	arg1	temperature					291:301	room temperature	286:301	room temperature	286:301	Thermo-induced shape-memory polymers (SMPs) have been widely investigated, but their biomedical applications are limited because their switching temperature does not fall completely within the range of room temperature to body temperature.
24607166	4	26	theme	thermal	751:757	arg1	microstructure					700:713	the microstructure	696:713	the microstructure	696:713	We also analyzed the mechanism of the water-triggered shape-memory effect by considering the microstructure, water contact angle, water uptake, thermal properties and static and dynamic mechanical properties of the composite.
24607166	4	26	theme	thermal	751:757	arg1	properties					759:768	thermal properties	751:768	thermal properties	751:768	We also analyzed the mechanism of the water-triggered shape-memory effect by considering the microstructure, water contact angle, water uptake, thermal properties and static and dynamic mechanical properties of the composite.
24607166	7	27	theme	shape-memory	1084:1095	arg1	composite					1097:1105	the water-induced shape-memory composite	1066:1105	the water-induced shape-memory composite	1066:1105	Therefore, the water-induced shape-memory composite can potentially be developed into a new smart medical device.
24607166	7	28	theme	medical	1153:1159	arg1	device					1161:1166	a new smart medical device	1141:1166	a new smart medical device	1141:1166	Therefore, the water-induced shape-memory composite can potentially be developed into a new smart medical device.
24607166	1	29	theme	temperature	291:301	arg1	range					277:281	the range	273:281	the range of room temperature to body temperature	273:321	Thermo-induced shape-memory polymers (SMPs) have been widely investigated, but their biomedical applications are limited because their switching temperature does not fall completely within the range of room temperature to body temperature.
24607166	3	30	theme	%	521:521	arg1	content					508:514	an MCC content	501:514	an MCC content of 35% (PDLLA-MCC-35)	501:536	We observed that the composite with an MCC content of 35% (PDLLA-MCC-35) exhibited a good shape-memory effect upon exposure to water at 37°C.
24607166	5	31	theme	analysis	869:876	arg1	results					837:843	The results	833:843	The results of in vitro degradation analysis	833:876	The results of in vitro degradation analysis demonstrate that the composites exhibited good biodegradation.
24607166	7	32	theme	new	1143:1145	arg1	device					1161:1166	a new smart medical device	1141:1166	a new smart medical device	1141:1166	Therefore, the water-induced shape-memory composite can potentially be developed into a new smart medical device.
24607166	1	33	theme	body	306:309	arg1	temperature					311:321	body temperature	306:321	body temperature	306:321	Thermo-induced shape-memory polymers (SMPs) have been widely investigated, but their biomedical applications are limited because their switching temperature does not fall completely within the range of room temperature to body temperature.
24607166	3	34	theme	good	550:553	arg1	effect					568:573	a good shape-memory effect	548:573	a good shape-memory effect	548:573	We observed that the composite with an MCC content of 35% (PDLLA-MCC-35) exhibited a good shape-memory effect upon exposure to water at 37°C.
24607166	1	35	theme	switching	219:227	arg1	temperature					229:239	their switching temperature	213:239	their switching temperature	213:239	Thermo-induced shape-memory polymers (SMPs) have been widely investigated, but their biomedical applications are limited because their switching temperature does not fall completely within the range of room temperature to body temperature.
24607166	4	36	theme	shape-memory	661:672	arg1	effect					674:679	the water-triggered shape-memory effect	641:679	the water-triggered shape-memory effect	641:679	We also analyzed the mechanism of the water-triggered shape-memory effect by considering the microstructure, water contact angle, water uptake, thermal properties and static and dynamic mechanical properties of the composite.
24607166	4	37	theme	dynamic	785:791	arg1	properties					804:813	static and dynamic mechanical properties	774:813	static and dynamic mechanical properties	774:813	We also analyzed the mechanism of the water-triggered shape-memory effect by considering the microstructure, water contact angle, water uptake, thermal properties and static and dynamic mechanical properties of the composite.
24607166	4	37	theme	dynamic	785:791	arg1	microstructure					700:713	the microstructure	696:713	the microstructure	696:713	We also analyzed the mechanism of the water-triggered shape-memory effect by considering the microstructure, water contact angle, water uptake, thermal properties and static and dynamic mechanical properties of the composite.
24607166	4	38	theme	static	774:779	arg1	properties					804:813	static and dynamic mechanical properties	774:813	static and dynamic mechanical properties	774:813	We also analyzed the mechanism of the water-triggered shape-memory effect by considering the microstructure, water contact angle, water uptake, thermal properties and static and dynamic mechanical properties of the composite.
24607166	4	38	theme	static	774:779	arg1	microstructure					700:713	the microstructure	696:713	the microstructure	696:713	We also analyzed the mechanism of the water-triggered shape-memory effect by considering the microstructure, water contact angle, water uptake, thermal properties and static and dynamic mechanical properties of the composite.
24607166	4	39	theme	water-triggered	645:659	arg1	effect					674:679	the water-triggered shape-memory effect	641:679	the water-triggered shape-memory effect	641:679	We also analyzed the mechanism of the water-triggered shape-memory effect by considering the microstructure, water contact angle, water uptake, thermal properties and static and dynamic mechanical properties of the composite.
24607166	0	40	dep	poly	27:30	arg1	L-lactide					34:42	L-lactide	34:42	L-lactide	34:42	Water-induced shape-memory poly(D,L-lactide)/microcrystalline cellulose composites.
24607166	0	40	dep	poly	27:30	arg1	D					32:32	D	32:32	D	32:32	Water-induced shape-memory poly(D,L-lactide)/microcrystalline cellulose composites.
24607166	4	41	theme	effect	674:679	arg1	mechanism					628:636	the mechanism	624:636	the mechanism of the water-triggered shape-memory effect	624:679	We also analyzed the mechanism of the water-triggered shape-memory effect by considering the microstructure, water contact angle, water uptake, thermal properties and static and dynamic mechanical properties of the composite.
24607166	0	42	theme	cellulose	62:70	arg1	composites					72:81	cellulose composites	62:81	cellulose composites	62:81	Water-induced shape-memory poly(D,L-lactide)/microcrystalline cellulose composites.
24607166	3	43	theme	shape-memory	555:566	arg1	effect					568:573	a good shape-memory effect	548:573	a good shape-memory effect	548:573	We observed that the composite with an MCC content of 35% (PDLLA-MCC-35) exhibited a good shape-memory effect upon exposure to water at 37°C.
24607166	2	44	theme	microcrystalline	401:416	arg1	MCC					429:431	MCC	429:431	MCC	429:431	In this study, we prepared water-induced shape-memory composites composed of microcrystalline cellulose (MCC) and poly(d,l-lactide) (PDLLA).
24607166	2	44	theme	microcrystalline	401:416	arg1	cellulose					418:426	microcrystalline cellulose	401:426	microcrystalline cellulose (MCC)	401:432	In this study, we prepared water-induced shape-memory composites composed of microcrystalline cellulose (MCC) and poly(d,l-lactide) (PDLLA).
24607166	2	45	theme	shape-memory	365:376	arg1	composites					378:387	water-induced shape-memory composites	351:387	water-induced shape-memory composites composed of microcrystalline cellulose (MCC) and poly(d,l-lactide) (PDLLA)	351:462	In this study, we prepared water-induced shape-memory composites composed of microcrystalline cellulose (MCC) and poly(d,l-lactide) (PDLLA).
27259648	2	0	theme	hepatic	585:591	arg1	status					605:610	the hepatic antioxidant status	581:610	the hepatic antioxidant status	581:610	The results showed that the IPS-2 had superior hepatoprotective effects by increasing the serum enzyme activities and bilirubin (BIL) levels, decreasing the serum albumin (ALB) and triglyceride (TG) levels, improving the hepatic antioxidant status, and ameliorating the hepatic structure damage.
27259648	1	1	theme	cellulose	242:250	arg1	chromatography					252:265	DEAE-52 cellulose chromatography	234:265	DEAE-52 cellulose chromatography	234:265	In present study, the intracellular polysaccharide (IPS) and its two fractions of IPS-1 and IPS-2 were obtained and purified by DEAE-52 cellulose chromatography from Pleurotus eryngii SI-04 mycelia, and their hepatoprotective effects were also investigated.
27259648	4	2	theme	natural	854:860	arg1	drugs					862:866	natural drugs	854:866	natural drugs	854:866	These conclusions demonstrated that the both IPS and its fractions might be suitable for functional foods and natural drugs in preventing the acute liver damage.
27259648	2	3	theme	structure	642:650	arg1	damage					652:657	the hepatic structure damage	630:657	the hepatic structure damage	630:657	The results showed that the IPS-2 had superior hepatoprotective effects by increasing the serum enzyme activities and bilirubin (BIL) levels, decreasing the serum albumin (ALB) and triglyceride (TG) levels, improving the hepatic antioxidant status, and ameliorating the hepatic structure damage.
27259648	3	4	theme	monosaccharide	677:690	arg1	composition					692:702	the monosaccharide composition	673:702	the monosaccharide composition	673:702	Furthermore, the monosaccharide composition and main bond types were also analyzed.
27259648	2	5	theme	hepatic	634:640	arg1	damage					652:657	the hepatic structure damage	630:657	the hepatic structure damage	630:657	The results showed that the IPS-2 had superior hepatoprotective effects by increasing the serum enzyme activities and bilirubin (BIL) levels, decreasing the serum albumin (ALB) and triglyceride (TG) levels, improving the hepatic antioxidant status, and ameliorating the hepatic structure damage.
27259648	2	6	theme	albumin	527:533	arg1	levels					563:568	the serum albumin (ALB) and triglyceride (TG) levels	517:568	the serum albumin (ALB) and triglyceride (TG) levels	517:568	The results showed that the IPS-2 had superior hepatoprotective effects by increasing the serum enzyme activities and bilirubin (BIL) levels, decreasing the serum albumin (ALB) and triglyceride (TG) levels, improving the hepatic antioxidant status, and ameliorating the hepatic structure damage.
27259648	1	7	theme	Pleurotus	272:280	arg1	mycelia					296:302	Pleurotus eryngii SI-04 mycelia	272:302	Pleurotus eryngii SI-04 mycelia	272:302	In present study, the intracellular polysaccharide (IPS) and its two fractions of IPS-1 and IPS-2 were obtained and purified by DEAE-52 cellulose chromatography from Pleurotus eryngii SI-04 mycelia, and their hepatoprotective effects were also investigated.
27259648	2	8	theme	serum	521:525	arg1	ALB					536:538	ALB	536:538	ALB	536:538	The results showed that the IPS-2 had superior hepatoprotective effects by increasing the serum enzyme activities and bilirubin (BIL) levels, decreasing the serum albumin (ALB) and triglyceride (TG) levels, improving the hepatic antioxidant status, and ameliorating the hepatic structure damage.
27259648	2	8	theme	serum	521:525	arg1	albumin					527:533	serum albumin	521:533	serum albumin (ALB)	521:539	The results showed that the IPS-2 had superior hepatoprotective effects by increasing the serum enzyme activities and bilirubin (BIL) levels, decreasing the serum albumin (ALB) and triglyceride (TG) levels, improving the hepatic antioxidant status, and ameliorating the hepatic structure damage.
27259648	4	9	theme	functional	833:842	arg1	foods					844:848	functional foods	833:848	functional foods	833:848	These conclusions demonstrated that the both IPS and its fractions might be suitable for functional foods and natural drugs in preventing the acute liver damage.
27259648	2	10	theme	superior	402:409	arg1	effects					428:434	superior hepatoprotective effects	402:434	superior hepatoprotective effects	402:434	The results showed that the IPS-2 had superior hepatoprotective effects by increasing the serum enzyme activities and bilirubin (BIL) levels, decreasing the serum albumin (ALB) and triglyceride (TG) levels, improving the hepatic antioxidant status, and ameliorating the hepatic structure damage.
27259648	3	11	theme	main	708:711	arg1	types					718:722	main bond types	708:722	main bond types	708:722	Furthermore, the monosaccharide composition and main bond types were also analyzed.
27259648	2	12	theme	enzyme	460:465	arg1	activities					467:476	the serum enzyme activities	450:476	the serum enzyme activities	450:476	The results showed that the IPS-2 had superior hepatoprotective effects by increasing the serum enzyme activities and bilirubin (BIL) levels, decreasing the serum albumin (ALB) and triglyceride (TG) levels, improving the hepatic antioxidant status, and ameliorating the hepatic structure damage.
27259648	3	13	theme	bond	713:716	arg1	types					718:722	main bond types	708:722	main bond types	708:722	Furthermore, the monosaccharide composition and main bond types were also analyzed.
27259648	0	14	theme	Antioxidant	0:10	arg1	activities					33:42	Antioxidant and hepatoprotective activities	0:42	Antioxidant and hepatoprotective activities of intracellular polysaccharide from Pleurotus eryngii SI-04.	0:104	Antioxidant and hepatoprotective activities of intracellular polysaccharide from Pleurotus eryngii SI-04.
27259648	2	15	theme	antioxidant	593:603	arg1	status					605:610	the hepatic antioxidant status	581:610	the hepatic antioxidant status	581:610	The results showed that the IPS-2 had superior hepatoprotective effects by increasing the serum enzyme activities and bilirubin (BIL) levels, decreasing the serum albumin (ALB) and triglyceride (TG) levels, improving the hepatic antioxidant status, and ameliorating the hepatic structure damage.
27259648	1	16	theme	present	109:115	arg1	study					117:121	present study	109:121	present study	109:121	In present study, the intracellular polysaccharide (IPS) and its two fractions of IPS-1 and IPS-2 were obtained and purified by DEAE-52 cellulose chromatography from Pleurotus eryngii SI-04 mycelia, and their hepatoprotective effects were also investigated.
27259648	1	17	theme	IPS-1	188:192	arg1	fractions					175:183	its two fractions	167:183	its two fractions of IPS-1 and IPS-2	167:202	In present study, the intracellular polysaccharide (IPS) and its two fractions of IPS-1 and IPS-2 were obtained and purified by DEAE-52 cellulose chromatography from Pleurotus eryngii SI-04 mycelia, and their hepatoprotective effects were also investigated.
27259648	1	17	theme	IPS-1	188:192	arg1	IPS					158:160	the intracellular polysaccharide (IPS)	124:161	the intracellular polysaccharide (IPS)	124:161	In present study, the intracellular polysaccharide (IPS) and its two fractions of IPS-1 and IPS-2 were obtained and purified by DEAE-52 cellulose chromatography from Pleurotus eryngii SI-04 mycelia, and their hepatoprotective effects were also investigated.
27259648	2	18	theme	serum	454:458	arg1	activities					467:476	the serum enzyme activities	450:476	the serum enzyme activities	450:476	The results showed that the IPS-2 had superior hepatoprotective effects by increasing the serum enzyme activities and bilirubin (BIL) levels, decreasing the serum albumin (ALB) and triglyceride (TG) levels, improving the hepatic antioxidant status, and ameliorating the hepatic structure damage.
27259648	1	19	theme	SI-04	290:294	arg1	mycelia					296:302	Pleurotus eryngii SI-04 mycelia	272:302	Pleurotus eryngii SI-04 mycelia	272:302	In present study, the intracellular polysaccharide (IPS) and its two fractions of IPS-1 and IPS-2 were obtained and purified by DEAE-52 cellulose chromatography from Pleurotus eryngii SI-04 mycelia, and their hepatoprotective effects were also investigated.
27259648	2	20	theme	triglyceride	545:556	arg1	levels					563:568	the serum albumin (ALB) and triglyceride (TG) levels	517:568	the serum albumin (ALB) and triglyceride (TG) levels	517:568	The results showed that the IPS-2 had superior hepatoprotective effects by increasing the serum enzyme activities and bilirubin (BIL) levels, decreasing the serum albumin (ALB) and triglyceride (TG) levels, improving the hepatic antioxidant status, and ameliorating the hepatic structure damage.
27259648	0	21	theme	hepatoprotective	16:31	arg1	activities					33:42	Antioxidant and hepatoprotective activities	0:42	Antioxidant and hepatoprotective activities of intracellular polysaccharide from Pleurotus eryngii SI-04.	0:104	Antioxidant and hepatoprotective activities of intracellular polysaccharide from Pleurotus eryngii SI-04.
27259648	1	22	theme	IPS-2	198:202	arg1	fractions					175:183	its two fractions	167:183	its two fractions of IPS-1 and IPS-2	167:202	In present study, the intracellular polysaccharide (IPS) and its two fractions of IPS-1 and IPS-2 were obtained and purified by DEAE-52 cellulose chromatography from Pleurotus eryngii SI-04 mycelia, and their hepatoprotective effects were also investigated.
27259648	1	22	theme	IPS-2	198:202	arg1	IPS					158:160	the intracellular polysaccharide (IPS)	124:161	the intracellular polysaccharide (IPS)	124:161	In present study, the intracellular polysaccharide (IPS) and its two fractions of IPS-1 and IPS-2 were obtained and purified by DEAE-52 cellulose chromatography from Pleurotus eryngii SI-04 mycelia, and their hepatoprotective effects were also investigated.
27259648	0	23	theme	intracellular	47:59	arg1	polysaccharide					61:74	intracellular polysaccharide	47:74	intracellular polysaccharide from Pleurotus eryngii SI-04	47:103	Antioxidant and hepatoprotective activities of intracellular polysaccharide from Pleurotus eryngii SI-04.
27259648	0	24	from	SI-04	99:103	arg1	activities					33:42	Antioxidant and hepatoprotective activities	0:42	Antioxidant and hepatoprotective activities of intracellular polysaccharide from Pleurotus eryngii SI-04.	0:104	Antioxidant and hepatoprotective activities of intracellular polysaccharide from Pleurotus eryngii SI-04.
27259648	0	24	from	SI-04	99:103	arg1	polysaccharide					61:74	intracellular polysaccharide	47:74	intracellular polysaccharide from Pleurotus eryngii SI-04	47:103	Antioxidant and hepatoprotective activities of intracellular polysaccharide from Pleurotus eryngii SI-04.
27259648	2	25	contain	had	398:400	arg2	effects					428:434	superior hepatoprotective effects	402:434	superior hepatoprotective effects	402:434	The results showed that the IPS-2 had superior hepatoprotective effects by increasing the serum enzyme activities and bilirubin (BIL) levels, decreasing the serum albumin (ALB) and triglyceride (TG) levels, improving the hepatic antioxidant status, and ameliorating the hepatic structure damage.
27259648	2	25	contain	had	398:400	arg1	IPS-2					392:396	the IPS-2	388:396	the IPS-2	388:396	The results showed that the IPS-2 had superior hepatoprotective effects by increasing the serum enzyme activities and bilirubin (BIL) levels, decreasing the serum albumin (ALB) and triglyceride (TG) levels, improving the hepatic antioxidant status, and ameliorating the hepatic structure damage.
27259648	2	26	theme	hepatoprotective	411:426	arg1	effects					428:434	superior hepatoprotective effects	402:434	superior hepatoprotective effects	402:434	The results showed that the IPS-2 had superior hepatoprotective effects by increasing the serum enzyme activities and bilirubin (BIL) levels, decreasing the serum albumin (ALB) and triglyceride (TG) levels, improving the hepatic antioxidant status, and ameliorating the hepatic structure damage.
27259648	2	27	theme	bilirubin	482:490	arg1	levels					498:503	bilirubin (BIL) levels	482:503	bilirubin (BIL) levels	482:503	The results showed that the IPS-2 had superior hepatoprotective effects by increasing the serum enzyme activities and bilirubin (BIL) levels, decreasing the serum albumin (ALB) and triglyceride (TG) levels, improving the hepatic antioxidant status, and ameliorating the hepatic structure damage.
27259648	4	28	theme	acute	886:890	arg1	damage					898:903	the acute liver damage	882:903	the acute liver damage	882:903	These conclusions demonstrated that the both IPS and its fractions might be suitable for functional foods and natural drugs in preventing the acute liver damage.
27259648	0	29	theme	polysaccharide	61:74	arg1	activities					33:42	Antioxidant and hepatoprotective activities	0:42	Antioxidant and hepatoprotective activities of intracellular polysaccharide from Pleurotus eryngii SI-04.	0:104	Antioxidant and hepatoprotective activities of intracellular polysaccharide from Pleurotus eryngii SI-04.
27259648	1	30	theme	hepatoprotective	315:330	arg1	effects					332:338	their hepatoprotective effects	309:338	their hepatoprotective effects	309:338	In present study, the intracellular polysaccharide (IPS) and its two fractions of IPS-1 and IPS-2 were obtained and purified by DEAE-52 cellulose chromatography from Pleurotus eryngii SI-04 mycelia, and their hepatoprotective effects were also investigated.
27259648	1	31	theme	intracellular	128:140	arg1	IPS					158:160	the intracellular polysaccharide (IPS)	124:161	the intracellular polysaccharide (IPS)	124:161	In present study, the intracellular polysaccharide (IPS) and its two fractions of IPS-1 and IPS-2 were obtained and purified by DEAE-52 cellulose chromatography from Pleurotus eryngii SI-04 mycelia, and their hepatoprotective effects were also investigated.
27259648	1	32	dep	Pleurotus	272:280	arg1	eryngii					282:288	eryngii	282:288	eryngii	282:288	In present study, the intracellular polysaccharide (IPS) and its two fractions of IPS-1 and IPS-2 were obtained and purified by DEAE-52 cellulose chromatography from Pleurotus eryngii SI-04 mycelia, and their hepatoprotective effects were also investigated.
27259648	1	33	theme	polysaccharide	142:155	arg1	IPS					158:160	the intracellular polysaccharide (IPS)	124:161	the intracellular polysaccharide (IPS)	124:161	In present study, the intracellular polysaccharide (IPS) and its two fractions of IPS-1 and IPS-2 were obtained and purified by DEAE-52 cellulose chromatography from Pleurotus eryngii SI-04 mycelia, and their hepatoprotective effects were also investigated.
27259648	4	34	theme	liver	892:896	arg1	damage					898:903	the acute liver damage	882:903	the acute liver damage	882:903	These conclusions demonstrated that the both IPS and its fractions might be suitable for functional foods and natural drugs in preventing the acute liver damage.
27259648	0	35	from	activities	33:42	arg1	SI-04					99:103	SI-04	99:103	SI-04	99:103	Antioxidant and hepatoprotective activities of intracellular polysaccharide from Pleurotus eryngii SI-04.
27259648	1	36	theme	DEAE-52	234:240	arg1	chromatography					252:265	DEAE-52 cellulose chromatography	234:265	DEAE-52 cellulose chromatography	234:265	In present study, the intracellular polysaccharide (IPS) and its two fractions of IPS-1 and IPS-2 were obtained and purified by DEAE-52 cellulose chromatography from Pleurotus eryngii SI-04 mycelia, and their hepatoprotective effects were also investigated.
25739091	11	0	contain	had	1574:1576	arg2	no					1585:1586	no	1585:1586	no	1585:1586	O-antigen from most Typhimurium were O-acetylated on rhamnose and abequose residues, while Enteritidis O-antigen had low or no O-acetylation.
25739091	11	0	contain	had	1574:1576	arg2	low					1578:1580	low	1578:1580	low	1578:1580	O-antigen from most Typhimurium were O-acetylated on rhamnose and abequose residues, while Enteritidis O-antigen had low or no O-acetylation.
25739091	11	0	contain	had	1574:1576	arg1	O-antigen					1564:1572	Enteritidis O-antigen	1552:1572	Enteritidis O-antigen	1552:1572	O-antigen from most Typhimurium were O-acetylated on rhamnose and abequose residues, while Enteritidis O-antigen had low or no O-acetylation.
25739091	17	1	from	phenotype	2248:2256	arg1	blood					2275:2279	blood	2275:2279	blood	2275:2279	No clear differences were found in the phenotype of isolates from blood and stool, suggesting that the same isolates can cause invasive disease and gastroenteritis.
25739091	17	1	from	phenotype	2248:2256	arg1	stool					2285:2289	stool	2285:2289	stool	2285:2289	No clear differences were found in the phenotype of isolates from blood and stool, suggesting that the same isolates can cause invasive disease and gastroenteritis.
25739091	5	2	dep	FINDINGS	691:698	arg1	studied					703:709	studied	703:709	studied 192 NTS isolates (114 Typhimurium, 78 Enteritidis) from blood and stools, mostly from paediatric admissions in Kenya 2000-2011	703:836	METHODOLOGY/PRINCIPAL FINDINGS We studied 192 NTS isolates (114 Typhimurium, 78 Enteritidis) from blood and stools, mostly from paediatric admissions in Kenya 2000-2011.
25739091	6	3	theme	whole	915:919	arg1	serum					927:931	whole adult serum	915:931	whole adult serum	915:931	Isolates were tested for susceptibility to antibody-mediated killing, using whole adult serum.
25739091	13	4	theme	O-antigen	1726:1734	arg1	level					1750:1754	O-antigen glucosylation level	1726:1754	O-antigen glucosylation level	1726:1754	Amount of S. Typhimurium O-antigen and O-antigen glucosylation level were inversely related.
25739091	14	5	theme	O-antigen	1870:1878	arg1	level					1880:1884	O-antigen level	1870:1884	O-antigen level	1870:1884	There was no clear association between clinical presentation and antibody susceptibility, O-antigen level or other O-antigen features.
25739091	17	6	theme	isolates	2261:2268	arg1	phenotype					2248:2256	the phenotype	2244:2256	the phenotype of isolates from blood and stool	2244:2289	No clear differences were found in the phenotype of isolates from blood and stool, suggesting that the same isolates can cause invasive disease and gastroenteritis.
25739091	14	7	theme	other	1889:1893	arg1	features					1905:1912	other O-antigen features	1889:1912	other O-antigen features	1889:1912	There was no clear association between clinical presentation and antibody susceptibility, O-antigen level or other O-antigen features.
25739091	13	8	theme	level	1750:1754	arg1	O-antigen					1712:1720	S. Typhimurium O-antigen	1697:1720	S. Typhimurium O-antigen	1697:1720	Amount of S. Typhimurium O-antigen and O-antigen glucosylation level were inversely related.
25739091	13	8	theme	level	1750:1754	arg1	level					1750:1754	O-antigen glucosylation level	1726:1754	O-antigen glucosylation level	1726:1754	Amount of S. Typhimurium O-antigen and O-antigen glucosylation level were inversely related.
25739091	13	8	theme	level	1750:1754	arg1	Amount					1687:1692	Amount	1687:1692	Amount of S. Typhimurium O-antigen and O-antigen glucosylation level	1687:1754	Amount of S. Typhimurium O-antigen and O-antigen glucosylation level were inversely related.
25739091	6	9	theme	antibody-mediated	882:898	arg1	killing					900:906	antibody-mediated killing	882:906	antibody-mediated killing	882:906	Isolates were tested for susceptibility to antibody-mediated killing, using whole adult serum.
25739091	13	10	theme	S.	1697:1698	arg1	O-antigen					1712:1720	S. Typhimurium O-antigen	1697:1720	S. Typhimurium O-antigen	1697:1720	Amount of S. Typhimurium O-antigen and O-antigen glucosylation level were inversely related.
25739091	7	11	theme	structural	944:953	arg1	glucosylation					1000:1012	glucosylation	1000:1012	glucosylation	1000:1012	O-antigen structural characteristics, including O-acetylation and glucosylation, were investigated.
25739091	7	11	theme	structural	944:953	arg1	characteristics					955:969	O-antigen structural characteristics	934:969	O-antigen structural characteristics	934:969	O-antigen structural characteristics, including O-acetylation and glucosylation, were investigated.
25739091	7	11	theme	structural	944:953	arg1	O-acetylation					982:994	O-acetylation	982:994	O-acetylation	982:994	O-antigen structural characteristics, including O-acetylation and glucosylation, were investigated.
25739091	18	12	theme	Genome	2374:2379	arg1	studies					2381:2387	Genome studies	2374:2387	Genome studies	2374:2387	Genome studies are required to understand whether invasive and gastrointestinal isolates differ at the genotypic level.
25739091	15	13	theme	O-antigen	2093:2101	arg1	level					2084:2088	level	2084:2088	level of O-antigen	2084:2101	CONCLUSION/SIGNIFICANCE Kenyan S. Typhimurium and Enteritidis clinical isolates are susceptible to antibody-mediated killing, with degree of susceptibility varying with level of O-antigen for S. Typhimurium.
25739091	5	14	theme	paediatric	797:806	arg1	admissions					808:817	paediatric admissions	797:817	paediatric admissions in Kenya 2000-2011	797:836	METHODOLOGY/PRINCIPAL FINDINGS We studied 192 NTS isolates (114 Typhimurium, 78 Enteritidis) from blood and stools, mostly from paediatric admissions in Kenya 2000-2011.
25739091	13	15	theme	O-antigen	1712:1720	arg1	O-antigen					1712:1720	S. Typhimurium O-antigen	1697:1720	S. Typhimurium O-antigen	1697:1720	Amount of S. Typhimurium O-antigen and O-antigen glucosylation level were inversely related.
25739091	13	15	theme	O-antigen	1712:1720	arg1	level					1750:1754	O-antigen glucosylation level	1726:1754	O-antigen glucosylation level	1726:1754	Amount of S. Typhimurium O-antigen and O-antigen glucosylation level were inversely related.
25739091	13	15	theme	O-antigen	1712:1720	arg1	Amount					1687:1692	Amount	1687:1692	Amount of S. Typhimurium O-antigen and O-antigen glucosylation level	1687:1754	Amount of S. Typhimurium O-antigen and O-antigen glucosylation level were inversely related.
25739091	1	16	theme	invasive	239:246	arg1	disease					269:275	invasive and gastrointestinal disease	239:275	invasive and gastrointestinal disease	239:275	BACKGROUND Nontyphoidal Salmonellae (NTS) cause a large burden of invasive and gastrointestinal disease among young children in sub-Saharan Africa.
25739091	14	17	theme	antibody	1845:1852	arg1	susceptibility					1854:1867	antibody susceptibility	1845:1867	antibody susceptibility	1845:1867	There was no clear association between clinical presentation and antibody susceptibility, O-antigen level or other O-antigen features.
25739091	1	18	theme	gastrointestinal	252:267	arg1	disease					269:275	invasive and gastrointestinal disease	239:275	invasive and gastrointestinal disease	239:275	BACKGROUND Nontyphoidal Salmonellae (NTS) cause a large burden of invasive and gastrointestinal disease among young children in sub-Saharan Africa.
25739091	15	19	theme	antibody-mediated	2014:2030	arg1	killing					2032:2038	antibody-mediated killing	2014:2038	antibody-mediated killing	2014:2038	CONCLUSION/SIGNIFICANCE Kenyan S. Typhimurium and Enteritidis clinical isolates are susceptible to antibody-mediated killing, with degree of susceptibility varying with level of O-antigen for S. Typhimurium.
25739091	3	20	theme	Previous	356:363	arg1	reports					365:371	Previous reports	356:371	Previous reports	356:371	Previous reports indicate the importance of the O-antigen of Salmonella lipopolysaccharide for virulence and resistance to antibody-mediated killing.
25739091	4	21	theme	more	541:544	arg1	O-antigen					546:554	more O-antigen	541:554	more O-antigen	541:554	We hypothesised that isolates with more O-antigen have increased resistance to antibody-mediated killing and are more likely to be invasive than gastrointestinal.
25739091	17	22	theme	clear	2212:2216	arg1	differences					2218:2228	No clear differences	2209:2228	No clear differences	2209:2228	No clear differences were found in the phenotype of isolates from blood and stool, suggesting that the same isolates can cause invasive disease and gastroenteritis.
25739091	1	23	from	children	289:296	arg1	Africa					313:318	Africa	313:318	Africa	313:318	BACKGROUND Nontyphoidal Salmonellae (NTS) cause a large burden of invasive and gastrointestinal disease among young children in sub-Saharan Africa.
25739091	11	24	from	Typhimurium	1481:1491	arg1	O-antigen					1461:1469	O-antigen	1461:1469	O-antigen from most Typhimurium	1461:1491	O-antigen from most Typhimurium were O-acetylated on rhamnose and abequose residues, while Enteritidis O-antigen had low or no O-acetylation.
25739091	9	25	theme	=	1361:1361	arg1	p					1359:1359	p = 0.002	1359:1367	p = 0.002	1359:1367	For S. Typhimurium, but not Enteritidis, O-antigen expression correlated with reduced sensitivity to killing (r = 0.29, 95% CI = 0.10-0.45, p = 0.002).
25739091	9	26	dep	r	1329:1329	arg1	CI					1343:1344	95% CI = 0.10-0.45	1339:1356	95% CI = 0.10-0.45	1339:1356	For S. Typhimurium, but not Enteritidis, O-antigen expression correlated with reduced sensitivity to killing (r = 0.29, 95% CI = 0.10-0.45, p = 0.002).
25739091	9	26	dep	r	1329:1329	arg1	p					1359:1359	p = 0.002	1359:1367	p = 0.002	1359:1367	For S. Typhimurium, but not Enteritidis, O-antigen expression correlated with reduced sensitivity to killing (r = 0.29, 95% CI = 0.10-0.45, p = 0.002).
25739091	15	27	dep	Kenyan	1939:1944	arg1	Typhimurium					1949:1959	CONCLUSION/SIGNIFICANCE Kenyan S. Typhimurium	1915:1959	CONCLUSION/SIGNIFICANCE Kenyan S. Typhimurium	1915:1959	CONCLUSION/SIGNIFICANCE Kenyan S. Typhimurium and Enteritidis clinical isolates are susceptible to antibody-mediated killing, with degree of susceptibility varying with level of O-antigen for S. Typhimurium.
25739091	1	28	theme	Nontyphoidal	184:195	arg1	NTS					210:212	NTS	210:212	NTS	210:212	BACKGROUND Nontyphoidal Salmonellae (NTS) cause a large burden of invasive and gastrointestinal disease among young children in sub-Saharan Africa.
25739091	1	28	theme	Nontyphoidal	184:195	arg1	Salmonellae					197:207	BACKGROUND Nontyphoidal Salmonellae	173:207	BACKGROUND Nontyphoidal Salmonellae (NTS)	173:213	BACKGROUND Nontyphoidal Salmonellae (NTS) cause a large burden of invasive and gastrointestinal disease among young children in sub-Saharan Africa.
25739091	11	29	dep	low	1578:1580	arg1	O-acetylation					1588:1600	O-acetylation	1588:1600	O-acetylation	1588:1600	O-antigen from most Typhimurium were O-acetylated on rhamnose and abequose residues, while Enteritidis O-antigen had low or no O-acetylation.
25739091	14	30	theme	clear	1793:1797	arg1	association					1799:1809	no clear association	1790:1809	no clear association between clinical presentation and antibody susceptibility, O-antigen level or other O-antigen features	1790:1912	There was no clear association between clinical presentation and antibody susceptibility, O-antigen level or other O-antigen features.
25739091	4	31	theme	antibody-mediated	585:601	arg1	killing					603:609	antibody-mediated killing	585:609	antibody-mediated killing	585:609	We hypothesised that isolates with more O-antigen have increased resistance to antibody-mediated killing and are more likely to be invasive than gastrointestinal.
25739091	17	32	located	found	2235:2239	arg1	phenotype					2248:2256	the phenotype	2244:2256	the phenotype of isolates from blood and stool	2244:2289	No clear differences were found in the phenotype of isolates from blood and stool, suggesting that the same isolates can cause invasive disease and gastroenteritis.
25739091	17	32	located	found	2235:2239	arg2	differences					2218:2228	No clear differences	2209:2228	No clear differences	2209:2228	No clear differences were found in the phenotype of isolates from blood and stool, suggesting that the same isolates can cause invasive disease and gastroenteritis.
25739091	5	33	theme	192	711:713	arg1	isolates					719:726	192 NTS isolates	711:726	192 NTS isolates (114 Typhimurium, 78 Enteritidis)	711:760	METHODOLOGY/PRINCIPAL FINDINGS We studied 192 NTS isolates (114 Typhimurium, 78 Enteritidis) from blood and stools, mostly from paediatric admissions in Kenya 2000-2011.
25739091	0	34	theme	nontyphoidal	127:138	arg1	isolates					152:159	invasive and gastrointestinal nontyphoidal Salmonellae isolates	97:159	invasive and gastrointestinal nontyphoidal Salmonellae isolates from Kenya	97:170	Relationship between antibody susceptibility and lipopolysaccharide O-antigen characteristics of invasive and gastrointestinal nontyphoidal Salmonellae isolates from Kenya.
25739091	16	35	theme	antibody-inducing	2159:2175	arg1	vaccine					2177:2183	an antibody-inducing vaccine	2156:2183	an antibody-inducing vaccine against NTS for Africa	2156:2206	This supports the development of an antibody-inducing vaccine against NTS for Africa.
25739091	12	36	theme	%	1667:1667	arg1	%					1671:1671	approximately 20%-50%	1651:1671	approximately 20%-50%	1651:1671	Both Typhimurium and Enteritidis O-antigen were approximately 20%-50% glucosylated.
25739091	0	37	theme	isolates	152:159	arg1	characteristics					78:92	antibody susceptibility and lipopolysaccharide O-antigen characteristics	21:92	antibody susceptibility and lipopolysaccharide O-antigen characteristics of invasive and gastrointestinal nontyphoidal Salmonellae isolates from Kenya	21:170	Relationship between antibody susceptibility and lipopolysaccharide O-antigen characteristics of invasive and gastrointestinal nontyphoidal Salmonellae isolates from Kenya.
25739091	5	38	theme	METHODOLOGY/PRINCIPAL	669:689	arg1	FINDINGS					691:698	METHODOLOGY/PRINCIPAL FINDINGS	669:698	METHODOLOGY/PRINCIPAL FINDINGS We studied 192 NTS isolates (114 Typhimurium, 78 Enteritidis) from blood and stools, mostly from paediatric admissions in Kenya 2000-2011.	669:837	METHODOLOGY/PRINCIPAL FINDINGS We studied 192 NTS isolates (114 Typhimurium, 78 Enteritidis) from blood and stools, mostly from paediatric admissions in Kenya 2000-2011.
25739091	15	39	theme	clinical	1977:1984	arg1	isolates					1986:1993	Enteritidis clinical isolates	1965:1993	Enteritidis clinical isolates	1965:1993	CONCLUSION/SIGNIFICANCE Kenyan S. Typhimurium and Enteritidis clinical isolates are susceptible to antibody-mediated killing, with degree of susceptibility varying with level of O-antigen for S. Typhimurium.
25739091	18	40	theme	genotypic	2477:2485	arg1	level					2487:2491	the genotypic level	2473:2491	the genotypic level	2473:2491	Genome studies are required to understand whether invasive and gastrointestinal isolates differ at the genotypic level.
25739091	3	41	theme	lipopolysaccharide	428:445	arg1	O-antigen					404:412	the O-antigen	400:412	the O-antigen of Salmonella lipopolysaccharide	400:445	Previous reports indicate the importance of the O-antigen of Salmonella lipopolysaccharide for virulence and resistance to antibody-mediated killing.
25739091	0	42	theme	antibody	21:28	arg1	susceptibility					30:43	antibody susceptibility	21:43	antibody susceptibility	21:43	Relationship between antibody susceptibility and lipopolysaccharide O-antigen characteristics of invasive and gastrointestinal nontyphoidal Salmonellae isolates from Kenya.
25739091	12	43	theme	Enteritidis	1624:1634	arg1	O-antigen					1636:1644	Both Typhimurium and Enteritidis O-antigen	1603:1644	Both Typhimurium and Enteritidis O-antigen	1603:1644	Both Typhimurium and Enteritidis O-antigen were approximately 20%-50% glucosylated.
25739091	3	44	theme	antibody-mediated	479:495	arg1	killing					497:503	antibody-mediated killing	479:503	antibody-mediated killing	479:503	Previous reports indicate the importance of the O-antigen of Salmonella lipopolysaccharide for virulence and resistance to antibody-mediated killing.
25739091	10	45	theme	O-antigen	1395:1403	arg1	populations					1405:1415	O-antigen populations	1395:1415	O-antigen populations ranging 21-33 kDa average molecular weight	1395:1458	Both serovars expressed O-antigen populations ranging 21-33 kDa average molecular weight.
25739091	10	46	theme	molecular	1443:1451	arg1	weight					1453:1458	21-33 kDa average molecular weight	1425:1458	21-33 kDa average molecular weight	1425:1458	Both serovars expressed O-antigen populations ranging 21-33 kDa average molecular weight.
25739091	0	47	theme	O-antigen	68:76	arg1	characteristics					78:92	antibody susceptibility and lipopolysaccharide O-antigen characteristics	21:92	antibody susceptibility and lipopolysaccharide O-antigen characteristics of invasive and gastrointestinal nontyphoidal Salmonellae isolates from Kenya	21:170	Relationship between antibody susceptibility and lipopolysaccharide O-antigen characteristics of invasive and gastrointestinal nontyphoidal Salmonellae isolates from Kenya.
25739091	8	48	theme	antibody-mediated	1072:1088	arg1	killing					1090:1096	antibody-mediated killing	1072:1096	antibody-mediated killing	1072:1096	Overall, isolates were susceptible to antibody-mediated killing, but S. Enteritidis were less susceptible and expressed more O-antigen than Typhimurium (p<0.0001 for both comparisons).
25739091	5	49	dep	isolates	719:726	arg1	Typhimurium					733:743	114 Typhimurium	729:743	114 Typhimurium	729:743	METHODOLOGY/PRINCIPAL FINDINGS We studied 192 NTS isolates (114 Typhimurium, 78 Enteritidis) from blood and stools, mostly from paediatric admissions in Kenya 2000-2011.
25739091	5	49	dep	isolates	719:726	arg1	Enteritidis					749:759	78 Enteritidis	746:759	78 Enteritidis	746:759	METHODOLOGY/PRINCIPAL FINDINGS We studied 192 NTS isolates (114 Typhimurium, 78 Enteritidis) from blood and stools, mostly from paediatric admissions in Kenya 2000-2011.
25739091	10	50	theme	kDa	1431:1433	arg1	weight					1453:1458	21-33 kDa average molecular weight	1425:1458	21-33 kDa average molecular weight	1425:1458	Both serovars expressed O-antigen populations ranging 21-33 kDa average molecular weight.
25739091	4	51	contain	have	556:559	arg1	isolates					527:534	isolates	527:534	isolates with more O-antigen	527:554	We hypothesised that isolates with more O-antigen have increased resistance to antibody-mediated killing and are more likely to be invasive than gastrointestinal.
25739091	4	51	contain	have	556:559	arg2	resistance					571:580	increased resistance	561:580	increased resistance to antibody-mediated killing	561:609	We hypothesised that isolates with more O-antigen have increased resistance to antibody-mediated killing and are more likely to be invasive than gastrointestinal.
25739091	1	52	theme	disease	269:275	arg1	burden					229:234	a large burden	221:234	a large burden of invasive and gastrointestinal disease among young children in sub-Saharan Africa	221:318	BACKGROUND Nontyphoidal Salmonellae (NTS) cause a large burden of invasive and gastrointestinal disease among young children in sub-Saharan Africa.
25739091	12	53	theme	Typhimurium	1608:1618	arg1	O-antigen					1636:1644	Both Typhimurium and Enteritidis O-antigen	1603:1644	Both Typhimurium and Enteritidis O-antigen	1603:1644	Both Typhimurium and Enteritidis O-antigen were approximately 20%-50% glucosylated.
25739091	9	54	theme	reduced	1297:1303	arg1	sensitivity					1305:1315	reduced sensitivity	1297:1315	reduced sensitivity to killing (r = 0.29, 95% CI = 0.10-0.45, p = 0.002)	1297:1368	For S. Typhimurium, but not Enteritidis, O-antigen expression correlated with reduced sensitivity to killing (r = 0.29, 95% CI = 0.10-0.45, p = 0.002).
25739091	1	55	theme	young	283:287	arg1	children					289:296	young children	283:296	young children in sub-Saharan Africa	283:318	BACKGROUND Nontyphoidal Salmonellae (NTS) cause a large burden of invasive and gastrointestinal disease among young children in sub-Saharan Africa.
25739091	0	56	from	Kenya	166:170	arg1	isolates					152:159	invasive and gastrointestinal nontyphoidal Salmonellae isolates	97:159	invasive and gastrointestinal nontyphoidal Salmonellae isolates from Kenya	97:170	Relationship between antibody susceptibility and lipopolysaccharide O-antigen characteristics of invasive and gastrointestinal nontyphoidal Salmonellae isolates from Kenya.
25739091	0	56	from	Kenya	166:170	arg1	characteristics					78:92	antibody susceptibility and lipopolysaccharide O-antigen characteristics	21:92	antibody susceptibility and lipopolysaccharide O-antigen characteristics of invasive and gastrointestinal nontyphoidal Salmonellae isolates from Kenya	21:170	Relationship between antibody susceptibility and lipopolysaccharide O-antigen characteristics of invasive and gastrointestinal nontyphoidal Salmonellae isolates from Kenya.
25739091	5	57	from	admissions	808:817	arg1	Kenya					822:826	Kenya 2000-2011	822:836	Kenya 2000-2011	822:836	METHODOLOGY/PRINCIPAL FINDINGS We studied 192 NTS isolates (114 Typhimurium, 78 Enteritidis) from blood and stools, mostly from paediatric admissions in Kenya 2000-2011.
25739091	5	57	from	admissions	808:817	arg1	isolates					719:726	192 NTS isolates	711:726	192 NTS isolates (114 Typhimurium, 78 Enteritidis)	711:760	METHODOLOGY/PRINCIPAL FINDINGS We studied 192 NTS isolates (114 Typhimurium, 78 Enteritidis) from blood and stools, mostly from paediatric admissions in Kenya 2000-2011.
25739091	13	58	dep	S.	1697:1698	arg1	Typhimurium					1700:1710	Typhimurium	1700:1710	Typhimurium	1700:1710	Amount of S. Typhimurium O-antigen and O-antigen glucosylation level were inversely related.
25739091	11	59	theme	abequose	1527:1534	arg1	residues					1536:1543	abequose residues	1527:1543	abequose residues	1527:1543	O-antigen from most Typhimurium were O-acetylated on rhamnose and abequose residues, while Enteritidis O-antigen had low or no O-acetylation.
25739091	6	60	theme	adult	921:925	arg1	serum					927:931	whole adult serum	915:931	whole adult serum	915:931	Isolates were tested for susceptibility to antibody-mediated killing, using whole adult serum.
25739091	9	61	theme	O-antigen	1260:1268	arg1	expression					1270:1279	O-antigen expression	1260:1279	O-antigen expression	1260:1279	For S. Typhimurium, but not Enteritidis, O-antigen expression correlated with reduced sensitivity to killing (r = 0.29, 95% CI = 0.10-0.45, p = 0.002).
25739091	8	62	dep	Typhimurium	1174:1184	arg1	p<0.0001					1187:1194	p<0.0001	1187:1194	p<0.0001 for both comparisons	1187:1215	Overall, isolates were susceptible to antibody-mediated killing, but S. Enteritidis were less susceptible and expressed more O-antigen than Typhimurium (p<0.0001 for both comparisons).
25739091	9	63	theme	=	1331:1331	arg1	r					1329:1329	r = 0.29	1329:1336	r = 0.29	1329:1336	For S. Typhimurium, but not Enteritidis, O-antigen expression correlated with reduced sensitivity to killing (r = 0.29, 95% CI = 0.10-0.45, p = 0.002).
25739091	9	63	theme	=	1331:1331	arg1	killing					1320:1326	killing	1320:1326	killing (r = 0.29, 95% CI = 0.10-0.45, p = 0.002)	1320:1368	For S. Typhimurium, but not Enteritidis, O-antigen expression correlated with reduced sensitivity to killing (r = 0.29, 95% CI = 0.10-0.45, p = 0.002).
25739091	13	64	theme	glucosylation	1736:1748	arg1	level					1750:1754	O-antigen glucosylation level	1726:1754	O-antigen glucosylation level	1726:1754	Amount of S. Typhimurium O-antigen and O-antigen glucosylation level were inversely related.
25739091	1	65	theme	large	223:227	arg1	burden					229:234	a large burden	221:234	a large burden of invasive and gastrointestinal disease among young children in sub-Saharan Africa	221:318	BACKGROUND Nontyphoidal Salmonellae (NTS) cause a large burden of invasive and gastrointestinal disease among young children in sub-Saharan Africa.
25739091	9	66	theme	%	1341:1341	arg1	CI					1343:1344	95% CI = 0.10-0.45	1339:1356	95% CI = 0.10-0.45	1339:1356	For S. Typhimurium, but not Enteritidis, O-antigen expression correlated with reduced sensitivity to killing (r = 0.29, 95% CI = 0.10-0.45, p = 0.002).
25739091	17	67	from	stool	2285:2289	arg1	isolates					2261:2268	isolates	2261:2268	isolates from blood and stool	2261:2289	No clear differences were found in the phenotype of isolates from blood and stool, suggesting that the same isolates can cause invasive disease and gastroenteritis.
25739091	17	67	from	stool	2285:2289	arg1	phenotype					2248:2256	the phenotype	2244:2256	the phenotype of isolates from blood and stool	2244:2289	No clear differences were found in the phenotype of isolates from blood and stool, suggesting that the same isolates can cause invasive disease and gastroenteritis.
25739091	9	68	theme	=	1346:1346	arg1	CI					1343:1344	95% CI = 0.10-0.45	1339:1356	95% CI = 0.10-0.45	1339:1356	For S. Typhimurium, but not Enteritidis, O-antigen expression correlated with reduced sensitivity to killing (r = 0.29, 95% CI = 0.10-0.45, p = 0.002).
25739091	7	69	theme	O-antigen	934:942	arg1	glucosylation					1000:1012	glucosylation	1000:1012	glucosylation	1000:1012	O-antigen structural characteristics, including O-acetylation and glucosylation, were investigated.
25739091	7	69	theme	O-antigen	934:942	arg1	characteristics					955:969	O-antigen structural characteristics	934:969	O-antigen structural characteristics	934:969	O-antigen structural characteristics, including O-acetylation and glucosylation, were investigated.
25739091	7	69	theme	O-antigen	934:942	arg1	O-acetylation					982:994	O-acetylation	982:994	O-acetylation	982:994	O-antigen structural characteristics, including O-acetylation and glucosylation, were investigated.
25739091	15	70	theme	susceptibility	2056:2069	arg1	degree					2046:2051	degree	2046:2051	degree of susceptibility varying with level of O-antigen for S. Typhimurium	2046:2120	CONCLUSION/SIGNIFICANCE Kenyan S. Typhimurium and Enteritidis clinical isolates are susceptible to antibody-mediated killing, with degree of susceptibility varying with level of O-antigen for S. Typhimurium.
25739091	14	71	theme	clinical	1819:1826	arg1	presentation					1828:1839	clinical presentation	1819:1839	clinical presentation	1819:1839	There was no clear association between clinical presentation and antibody susceptibility, O-antigen level or other O-antigen features.
25739091	1	72	theme	BACKGROUND	173:182	arg1	NTS					210:212	NTS	210:212	NTS	210:212	BACKGROUND Nontyphoidal Salmonellae (NTS) cause a large burden of invasive and gastrointestinal disease among young children in sub-Saharan Africa.
25739091	1	72	theme	BACKGROUND	173:182	arg1	Salmonellae					197:207	BACKGROUND Nontyphoidal Salmonellae	173:207	BACKGROUND Nontyphoidal Salmonellae (NTS)	173:213	BACKGROUND Nontyphoidal Salmonellae (NTS) cause a large burden of invasive and gastrointestinal disease among young children in sub-Saharan Africa.
25739091	0	73	theme	invasive	97:104	arg1	isolates					152:159	invasive and gastrointestinal nontyphoidal Salmonellae isolates	97:159	invasive and gastrointestinal nontyphoidal Salmonellae isolates from Kenya	97:170	Relationship between antibody susceptibility and lipopolysaccharide O-antigen characteristics of invasive and gastrointestinal nontyphoidal Salmonellae isolates from Kenya.
25739091	0	74	theme	gastrointestinal	110:125	arg1	isolates					152:159	invasive and gastrointestinal nontyphoidal Salmonellae isolates	97:159	invasive and gastrointestinal nontyphoidal Salmonellae isolates from Kenya	97:170	Relationship between antibody susceptibility and lipopolysaccharide O-antigen characteristics of invasive and gastrointestinal nontyphoidal Salmonellae isolates from Kenya.
25739091	0	75	from	characteristics	78:92	arg1	Kenya					166:170	Kenya	166:170	Kenya	166:170	Relationship between antibody susceptibility and lipopolysaccharide O-antigen characteristics of invasive and gastrointestinal nontyphoidal Salmonellae isolates from Kenya.
25739091	0	76	theme	Salmonellae	140:150	arg1	isolates					152:159	invasive and gastrointestinal nontyphoidal Salmonellae isolates	97:159	invasive and gastrointestinal nontyphoidal Salmonellae isolates from Kenya	97:170	Relationship between antibody susceptibility and lipopolysaccharide O-antigen characteristics of invasive and gastrointestinal nontyphoidal Salmonellae isolates from Kenya.
25739091	5	77	theme	NTS	715:717	arg1	isolates					719:726	192 NTS isolates	711:726	192 NTS isolates (114 Typhimurium, 78 Enteritidis)	711:760	METHODOLOGY/PRINCIPAL FINDINGS We studied 192 NTS isolates (114 Typhimurium, 78 Enteritidis) from blood and stools, mostly from paediatric admissions in Kenya 2000-2011.
25739091	16	78	theme	vaccine	2177:2183	arg1	development					2141:2151	the development	2137:2151	the development of an antibody-inducing vaccine against NTS for Africa	2137:2206	This supports the development of an antibody-inducing vaccine against NTS for Africa.
25739091	3	79	theme	Salmonella	417:426	arg1	lipopolysaccharide					428:445	Salmonella lipopolysaccharide	417:445	Salmonella lipopolysaccharide	417:445	Previous reports indicate the importance of the O-antigen of Salmonella lipopolysaccharide for virulence and resistance to antibody-mediated killing.
25739091	4	80	theme	increased	561:569	arg1	resistance					571:580	increased resistance	561:580	increased resistance to antibody-mediated killing	561:609	We hypothesised that isolates with more O-antigen have increased resistance to antibody-mediated killing and are more likely to be invasive than gastrointestinal.
25739091	11	81	theme	Enteritidis	1552:1562	arg1	O-antigen					1564:1572	Enteritidis O-antigen	1552:1572	Enteritidis O-antigen	1552:1572	O-antigen from most Typhimurium were O-acetylated on rhamnose and abequose residues, while Enteritidis O-antigen had low or no O-acetylation.
25739091	3	82	theme	O-antigen	404:412	arg1	importance					386:395	the importance	382:395	the importance of the O-antigen of Salmonella lipopolysaccharide for virulence and resistance to antibody-mediated killing	382:503	Previous reports indicate the importance of the O-antigen of Salmonella lipopolysaccharide for virulence and resistance to antibody-mediated killing.
25739091	15	83	theme	Enteritidis	1965:1975	arg1	isolates					1986:1993	Enteritidis clinical isolates	1965:1993	Enteritidis clinical isolates	1965:1993	CONCLUSION/SIGNIFICANCE Kenyan S. Typhimurium and Enteritidis clinical isolates are susceptible to antibody-mediated killing, with degree of susceptibility varying with level of O-antigen for S. Typhimurium.
25739091	10	84	theme	21-33	1425:1429	arg1	kDa					1431:1433	kDa	1431:1433	kDa	1431:1433	Both serovars expressed O-antigen populations ranging 21-33 kDa average molecular weight.
25739091	4	85	with	isolates	527:534	arg1	O-antigen					546:554	more O-antigen	541:554	more O-antigen	541:554	We hypothesised that isolates with more O-antigen have increased resistance to antibody-mediated killing and are more likely to be invasive than gastrointestinal.
25739091	0	86	theme	susceptibility	30:43	arg1	characteristics					78:92	antibody susceptibility and lipopolysaccharide O-antigen characteristics	21:92	antibody susceptibility and lipopolysaccharide O-antigen characteristics of invasive and gastrointestinal nontyphoidal Salmonellae isolates from Kenya	21:170	Relationship between antibody susceptibility and lipopolysaccharide O-antigen characteristics of invasive and gastrointestinal nontyphoidal Salmonellae isolates from Kenya.
25739091	0	87	theme	lipopolysaccharide	49:66	arg1	O-antigen					68:76	lipopolysaccharide O-antigen	49:76	lipopolysaccharide O-antigen	49:76	Relationship between antibody susceptibility and lipopolysaccharide O-antigen characteristics of invasive and gastrointestinal nontyphoidal Salmonellae isolates from Kenya.
25739091	15	88	with	susceptible	1999:2009	arg1	degree					2046:2051	degree	2046:2051	degree of susceptibility varying with level of O-antigen for S. Typhimurium	2046:2120	CONCLUSION/SIGNIFICANCE Kenyan S. Typhimurium and Enteritidis clinical isolates are susceptible to antibody-mediated killing, with degree of susceptibility varying with level of O-antigen for S. Typhimurium.
25739091	17	89	theme	same	2312:2315	arg1	isolates					2317:2324	the same isolates	2308:2324	the same isolates	2308:2324	No clear differences were found in the phenotype of isolates from blood and stool, suggesting that the same isolates can cause invasive disease and gastroenteritis.
25739091	5	90	from	blood	767:771	arg1	isolates					719:726	192 NTS isolates	711:726	192 NTS isolates (114 Typhimurium, 78 Enteritidis)	711:760	METHODOLOGY/PRINCIPAL FINDINGS We studied 192 NTS isolates (114 Typhimurium, 78 Enteritidis) from blood and stools, mostly from paediatric admissions in Kenya 2000-2011.
25739091	18	91	theme	gastrointestinal	2437:2452	arg1	isolates					2454:2461	invasive and gastrointestinal isolates	2424:2461	invasive and gastrointestinal isolates	2424:2461	Genome studies are required to understand whether invasive and gastrointestinal isolates differ at the genotypic level.
25739091	10	92	theme	average	1435:1441	arg1	weight					1453:1458	21-33 kDa average molecular weight	1425:1458	21-33 kDa average molecular weight	1425:1458	Both serovars expressed O-antigen populations ranging 21-33 kDa average molecular weight.
25739091	18	93	theme	invasive	2424:2431	arg1	isolates					2454:2461	invasive and gastrointestinal isolates	2424:2461	invasive and gastrointestinal isolates	2424:2461	Genome studies are required to understand whether invasive and gastrointestinal isolates differ at the genotypic level.
25739091	17	94	theme	invasive	2336:2343	arg1	disease					2345:2351	invasive disease	2336:2351	invasive disease	2336:2351	No clear differences were found in the phenotype of isolates from blood and stool, suggesting that the same isolates can cause invasive disease and gastroenteritis.
25739091	17	95	from	blood	2275:2279	arg1	isolates					2261:2268	isolates	2261:2268	isolates from blood and stool	2261:2289	No clear differences were found in the phenotype of isolates from blood and stool, suggesting that the same isolates can cause invasive disease and gastroenteritis.
25739091	17	95	from	blood	2275:2279	arg1	phenotype					2248:2256	the phenotype	2244:2256	the phenotype of isolates from blood and stool	2244:2289	No clear differences were found in the phenotype of isolates from blood and stool, suggesting that the same isolates can cause invasive disease and gastroenteritis.
25739091	5	96	from	stools	777:782	arg1	isolates					719:726	192 NTS isolates	711:726	192 NTS isolates (114 Typhimurium, 78 Enteritidis)	711:760	METHODOLOGY/PRINCIPAL FINDINGS We studied 192 NTS isolates (114 Typhimurium, 78 Enteritidis) from blood and stools, mostly from paediatric admissions in Kenya 2000-2011.
25739091	14	97	theme	O-antigen	1895:1903	arg1	features					1905:1912	other O-antigen features	1889:1912	other O-antigen features	1889:1912	There was no clear association between clinical presentation and antibody susceptibility, O-antigen level or other O-antigen features.
26415455	22	0	theme	immunomodulatory	2065:2080	arg1	activity					2082:2089	immunomodulatory activity	2065:2089	immunomodulatory activity assessed on the basis of nitric oxide production by RAW264	2065:2148	The polysaccharide samples had not exhibited immunomodulatory activity assessed on the basis of nitric oxide production by RAW264.
26415455	6	1	from	fruits	926:931	arg1	polysaccharides					884:898	the polysaccharides	880:898	the polysaccharides from Chaenomeles speciosa fruits	880:931	The aim of the study is to determine the amount and the molecular weight of the polysaccharides from Chaenomeles speciosa fruits, and preliminary investigate the immunomodulatory activity, The study provided the basis datas for the further research of Chaenomeles speciosa fruits.
26415455	6	1	from	fruits	926:931	arg1	amount					845:850	amount	845:850	amount	845:850	The aim of the study is to determine the amount and the molecular weight of the polysaccharides from Chaenomeles speciosa fruits, and preliminary investigate the immunomodulatory activity, The study provided the basis datas for the further research of Chaenomeles speciosa fruits.
26415455	6	1	from	fruits	926:931	arg1	weight					870:875	the molecular weight	856:875	the molecular weight	856:875	The aim of the study is to determine the amount and the molecular weight of the polysaccharides from Chaenomeles speciosa fruits, and preliminary investigate the immunomodulatory activity, The study provided the basis datas for the further research of Chaenomeles speciosa fruits.
26415455	11	2	theme	95	1559:1560	arg1	%					1554:1554	%	1554:1554	%	1554:1554	0% when the concentration of ethanol increased from 20% to 95%.
26415455	6	3	theme	Chaenomeles	905:915	arg1	fruits					926:931	Chaenomeles speciosa fruits	905:931	Chaenomeles speciosa fruits	905:931	The aim of the study is to determine the amount and the molecular weight of the polysaccharides from Chaenomeles speciosa fruits, and preliminary investigate the immunomodulatory activity, The study provided the basis datas for the further research of Chaenomeles speciosa fruits.
26415455	0	4	from	Precipitation	154:166	arg1	Investigation					13:25	[Preliminary Investigation	0:25	[Preliminary Investigation of the Amount, the Molecular Weight and the Activity of Polysaccharides from Chaenomeles Speciosa Fruits in Ethanol Fractional Precipitation	0:166	[Preliminary Investigation of the Amount, the Molecular Weight and the Activity of Polysaccharides from Chaenomeles Speciosa Fruits in Ethanol Fractional Precipitation].
26415455	19	5	theme	index	1971:1975	arg1	weight					1919:1924	The molecular weight	1905:1924	The molecular weight of the first two polysaccharide distribution index(Mw/Mn)	1905:1982	The molecular weight of the first two polysaccharide distribution index(Mw/Mn)were 1.
26415455	19	5	theme	index	1971:1975	arg1	1					1988:1988	1	1988:1988	1	1988:1988	The molecular weight of the first two polysaccharide distribution index(Mw/Mn)were 1.
26415455	8	6	theme	fractional	1183:1192	arg1	precipitation					1194:1206	The ethanol fractional precipitation	1171:1206	The ethanol fractional precipitation	1171:1206	The ethanol fractional precipitation showed that the order of total polysaccharide content was 95%>80%>40% ≥60%>20%.
26415455	6	7	theme	polysaccharides	884:898	arg1	polysaccharides					884:898	the polysaccharides	880:898	the polysaccharides from Chaenomeles speciosa fruits	880:931	The aim of the study is to determine the amount and the molecular weight of the polysaccharides from Chaenomeles speciosa fruits, and preliminary investigate the immunomodulatory activity, The study provided the basis datas for the further research of Chaenomeles speciosa fruits.
26415455	6	7	theme	polysaccharides	884:898	arg1	amount					845:850	amount	845:850	amount	845:850	The aim of the study is to determine the amount and the molecular weight of the polysaccharides from Chaenomeles speciosa fruits, and preliminary investigate the immunomodulatory activity, The study provided the basis datas for the further research of Chaenomeles speciosa fruits.
26415455	6	7	theme	polysaccharides	884:898	arg1	weight					870:875	the molecular weight	856:875	the molecular weight	856:875	The aim of the study is to determine the amount and the molecular weight of the polysaccharides from Chaenomeles speciosa fruits, and preliminary investigate the immunomodulatory activity, The study provided the basis datas for the further research of Chaenomeles speciosa fruits.
26415455	2	8	theme	%	268:268	arg1	ethanol					274:280	20%~95% (φ) ethanol	262:280	20%~95% (φ) ethanol	262:280	The extracts were precipitated with 20%~95% (φ) ethanol, respectively.
26415455	1	9	theme	speciosa	182:189	arg1	fruits					191:196	Chaenomeles speciosa fruits	170:196	Chaenomeles speciosa fruits	170:196	Chaenomeles speciosa fruits were extracted using water.
26415455	0	10	theme	Fractional	143:152	arg1	Precipitation					154:166	Ethanol Fractional Precipitation	135:166	Ethanol Fractional Precipitation	135:166	[Preliminary Investigation of the Amount, the Molecular Weight and the Activity of Polysaccharides from Chaenomeles Speciosa Fruits in Ethanol Fractional Precipitation].
26415455	8	11	theme	%	1268:1268	arg1	%					1281:1281	95%>80%>40% ≥60%	1266:1281	95%>80%>40% ≥60%>20%	1266:1285	The ethanol fractional precipitation showed that the order of total polysaccharide content was 95%>80%>40% ≥60%>20%.
26415455	11	12	theme	0	1500:1500	arg1	%					1501:1501	%	1501:1501	%	1501:1501	0% when the concentration of ethanol increased from 20% to 95%.
26415455	22	13	theme	oxide	2123:2127	arg1	production					2129:2138	nitric oxide production	2116:2138	nitric oxide production	2116:2138	The polysaccharide samples had not exhibited immunomodulatory activity assessed on the basis of nitric oxide production by RAW264.
26415455	8	14	theme	%	1272:1272	arg1	%					1281:1281	95%>80%>40% ≥60%	1266:1281	95%>80%>40% ≥60%>20%	1266:1285	The ethanol fractional precipitation showed that the order of total polysaccharide content was 95%>80%>40% ≥60%>20%.
26415455	13	15	theme	retention	1709:1717	arg1	time					1719:1722	the retention time	1705:1722	the retention time	1705:1722	They appeared three chromatographic peaks and the retention time were not apparently different.
26415455	13	15	theme	retention	1709:1717	arg1	different					1744:1752	different	1744:1752	different	1744:1752	They appeared three chromatographic peaks and the retention time were not apparently different.
26415455	0	16	theme	Speciosa	116:123	arg1	Fruits					125:130	Chaenomeles Speciosa Fruits	104:130	Chaenomeles Speciosa Fruits	104:130	[Preliminary Investigation of the Amount, the Molecular Weight and the Activity of Polysaccharides from Chaenomeles Speciosa Fruits in Ethanol Fractional Precipitation].
26415455	6	17	theme	immunomodulatory	966:981	arg1	activity					983:990	the immunomodulatory activity	962:990	the immunomodulatory activity	962:990	The aim of the study is to determine the amount and the molecular weight of the polysaccharides from Chaenomeles speciosa fruits, and preliminary investigate the immunomodulatory activity, The study provided the basis datas for the further research of Chaenomeles speciosa fruits.
26415455	8	18	theme	%	1276:1276	arg1	%					1281:1281	95%>80%>40% ≥60%	1266:1281	95%>80%>40% ≥60%>20%	1266:1285	The ethanol fractional precipitation showed that the order of total polysaccharide content was 95%>80%>40% ≥60%>20%.
26415455	5	19	theme	oxide	749:753	arg1	production					728:737	the production	724:737	the production of nitric oxide in the cells	724:766	RAW264.7 macrophage were cultured and stimulated with the polysaccharides in vitro and the production of nitric oxide in the cells was determined by the Griess assay.
26415455	9	20	theme	crude	1443:1447	arg1	purity					1464:1469	the crude polysaccharide purity	1439:1469	the crude polysaccharide purity	1439:1469	The results indicated that most polysaccharide from Chaenomeles speciosa fruits might be precipitated when ethanol concentration was up to 95% (T) and the crude polysaccharide purity had risen from 35.
26415455	8	21	theme	polysaccharide	1239:1252	arg1	content					1254:1260	total polysaccharide content	1233:1260	total polysaccharide content	1233:1260	The ethanol fractional precipitation showed that the order of total polysaccharide content was 95%>80%>40% ≥60%>20%.
26415455	0	22	from	Weight	56:61	arg1	Precipitation					154:166	Ethanol Fractional Precipitation	135:166	Ethanol Fractional Precipitation	135:166	[Preliminary Investigation of the Amount, the Molecular Weight and the Activity of Polysaccharides from Chaenomeles Speciosa Fruits in Ethanol Fractional Precipitation].
26415455	0	22	from	Weight	56:61	arg1	Fruits					125:130	Chaenomeles Speciosa Fruits	104:130	Chaenomeles Speciosa Fruits	104:130	[Preliminary Investigation of the Amount, the Molecular Weight and the Activity of Polysaccharides from Chaenomeles Speciosa Fruits in Ethanol Fractional Precipitation].
26415455	8	23	theme	%	1281:1281	arg1	%					1285:1285	95%>80%>40% ≥60%>20%	1266:1285	95%>80%>40% ≥60%>20%	1266:1285	The ethanol fractional precipitation showed that the order of total polysaccharide content was 95%>80%>40% ≥60%>20%.
26415455	8	23	theme	%	1281:1281	arg1	order					1224:1228	the order	1220:1228	the order of total polysaccharide content	1220:1260	The ethanol fractional precipitation showed that the order of total polysaccharide content was 95%>80%>40% ≥60%>20%.
26415455	13	24	theme	chromatographic	1679:1693	arg1	peaks					1695:1699	three chromatographic peaks	1673:1699	three chromatographic peaks	1673:1699	They appeared three chromatographic peaks and the retention time were not apparently different.
26415455	10	25	dep	%	1491:1491	arg1	to					1493:1494	to	1493:1494	to	1493:1494	1% to 45.
26415455	19	26	theme	distribution	1958:1969	arg1	Mw/Mn					1977:1981	Mw/Mn	1977:1981	Mw/Mn	1977:1981	The molecular weight of the first two polysaccharide distribution index(Mw/Mn)were 1.
26415455	19	26	theme	distribution	1958:1969	arg1	index					1971:1975	the first two polysaccharide distribution index	1929:1975	the first two polysaccharide distribution index(Mw/Mn)	1929:1982	The molecular weight of the first two polysaccharide distribution index(Mw/Mn)were 1.
26415455	12	27	theme	similar	1637:1643	arg1	compositions					1645:1656	the similar compositions	1633:1656	the similar compositions	1633:1656	HPSEC-MALLS-RI system showed that all the polysaccharide samples had the similar compositions.
26415455	4	28	theme	size-exclusion	411:424	arg1	HPSEC					442:446	HPSEC	442:446	HPSEC	442:446	A method using high-performance size-exclusion chromatography (HPSEC) equipped with multiangle laser-light-scattering photometry (MALLS) and differential refractometry (RI) was presented for determining the molecular weight and molecular weigh distribution.
26415455	4	28	theme	size-exclusion	411:424	arg1	chromatography					426:439	high-performance size-exclusion chromatography	394:439	high-performance size-exclusion chromatography (HPSEC) equipped with multiangle laser-light-scattering photometry (MALLS) and differential refractometry (RI)	394:550	A method using high-performance size-exclusion chromatography (HPSEC) equipped with multiangle laser-light-scattering photometry (MALLS) and differential refractometry (RI) was presented for determining the molecular weight and molecular weigh distribution.
26415455	15	29	dep	570	1770:1772	arg1	4					1779:1779	4	1779:1779	4	1779:1779	570 X 10(4) g .
26415455	2	30	theme	%	264:264	arg1	φ					271:271	φ	271:271	φ	271:271	The extracts were precipitated with 20%~95% (φ) ethanol, respectively.
26415455	2	30	theme	%	264:264	arg1	%					268:268	20%~95%	262:268	20%~95% (φ) ethanol	262:280	The extracts were precipitated with 20%~95% (φ) ethanol, respectively.
26415455	4	31	theme	molecular	607:615	arg1	distribution					623:634	molecular weigh distribution	607:634	molecular weigh distribution	607:634	A method using high-performance size-exclusion chromatography (HPSEC) equipped with multiangle laser-light-scattering photometry (MALLS) and differential refractometry (RI) was presented for determining the molecular weight and molecular weigh distribution.
26415455	12	32	theme	polysaccharide	1606:1619	arg1	samples					1621:1627	all the polysaccharide samples	1598:1627	all the polysaccharide samples	1598:1627	HPSEC-MALLS-RI system showed that all the polysaccharide samples had the similar compositions.
26415455	19	33	theme	polysaccharide	1943:1956	arg1	Mw/Mn					1977:1981	Mw/Mn	1977:1981	Mw/Mn	1977:1981	The molecular weight of the first two polysaccharide distribution index(Mw/Mn)were 1.
26415455	19	33	theme	polysaccharide	1943:1956	arg1	index					1971:1975	the first two polysaccharide distribution index	1929:1975	the first two polysaccharide distribution index(Mw/Mn)	1929:1982	The molecular weight of the first two polysaccharide distribution index(Mw/Mn)were 1.
26415455	22	34	dep	production	2129:2138	arg1	basis					2107:2111	basis	2107:2111	basis	2107:2111	The polysaccharide samples had not exhibited immunomodulatory activity assessed on the basis of nitric oxide production by RAW264.
26415455	22	34	dep	production	2129:2138	arg1	the					2103:2105	the	2103:2105	the	2103:2105	The polysaccharide samples had not exhibited immunomodulatory activity assessed on the basis of nitric oxide production by RAW264.
26415455	5	35	theme	Griess	790:795	arg1	assay					797:801	the Griess assay	786:801	the Griess assay	786:801	RAW264.7 macrophage were cultured and stimulated with the polysaccharides in vitro and the production of nitric oxide in the cells was determined by the Griess assay.
26415455	4	36	theme	differential	520:531	arg1	RI					548:549	RI	548:549	RI	548:549	A method using high-performance size-exclusion chromatography (HPSEC) equipped with multiangle laser-light-scattering photometry (MALLS) and differential refractometry (RI) was presented for determining the molecular weight and molecular weigh distribution.
26415455	4	36	theme	differential	520:531	arg1	refractometry					533:545	differential refractometry	520:545	differential refractometry (RI)	520:550	A method using high-performance size-exclusion chromatography (HPSEC) equipped with multiangle laser-light-scattering photometry (MALLS) and differential refractometry (RI) was presented for determining the molecular weight and molecular weigh distribution.
26415455	3	37	theme	polysaccharide	317:330	arg1	amount					301:306	The amount	297:306	The amount of total polysaccharide	297:330	The amount of total polysaccharide was measured with phenol-sulfuric acid method.
26415455	3	37	theme	polysaccharide	317:330	arg1	polysaccharide					317:330	total polysaccharide	311:330	total polysaccharide	311:330	The amount of total polysaccharide was measured with phenol-sulfuric acid method.
26415455	4	38	theme	laser-light-scattering	474:495	arg1	MALLS					509:513	MALLS	509:513	MALLS	509:513	A method using high-performance size-exclusion chromatography (HPSEC) equipped with multiangle laser-light-scattering photometry (MALLS) and differential refractometry (RI) was presented for determining the molecular weight and molecular weigh distribution.
26415455	4	38	theme	laser-light-scattering	474:495	arg1	photometry					497:506	multiangle laser-light-scattering photometry	463:506	multiangle laser-light-scattering photometry (MALLS)	463:514	A method using high-performance size-exclusion chromatography (HPSEC) equipped with multiangle laser-light-scattering photometry (MALLS) and differential refractometry (RI) was presented for determining the molecular weight and molecular weigh distribution.
26415455	0	39	from	Amount	34:39	arg1	Precipitation					154:166	Ethanol Fractional Precipitation	135:166	Ethanol Fractional Precipitation	135:166	[Preliminary Investigation of the Amount, the Molecular Weight and the Activity of Polysaccharides from Chaenomeles Speciosa Fruits in Ethanol Fractional Precipitation].
26415455	0	39	from	Amount	34:39	arg1	Fruits					125:130	Chaenomeles Speciosa Fruits	104:130	Chaenomeles Speciosa Fruits	104:130	[Preliminary Investigation of the Amount, the Molecular Weight and the Activity of Polysaccharides from Chaenomeles Speciosa Fruits in Ethanol Fractional Precipitation].
26415455	0	40	theme	[Preliminary	0:11	arg1	Investigation					13:25	[Preliminary Investigation	0:25	[Preliminary Investigation of the Amount, the Molecular Weight and the Activity of Polysaccharides from Chaenomeles Speciosa Fruits in Ethanol Fractional Precipitation	0:166	[Preliminary Investigation of the Amount, the Molecular Weight and the Activity of Polysaccharides from Chaenomeles Speciosa Fruits in Ethanol Fractional Precipitation].
26415455	6	41	theme	fruits	1077:1082	arg1	research					1044:1051	the further research	1032:1051	the further research of Chaenomeles speciosa fruits	1032:1082	The aim of the study is to determine the amount and the molecular weight of the polysaccharides from Chaenomeles speciosa fruits, and preliminary investigate the immunomodulatory activity, The study provided the basis datas for the further research of Chaenomeles speciosa fruits.
26415455	3	42	theme	phenol-sulfuric	350:364	arg1	method					371:376	phenol-sulfuric acid method	350:376	phenol-sulfuric acid method	350:376	The amount of total polysaccharide was measured with phenol-sulfuric acid method.
26415455	19	43	theme	molecular	1909:1917	arg1	weight					1919:1924	The molecular weight	1905:1924	The molecular weight of the first two polysaccharide distribution index(Mw/Mn)	1905:1982	The molecular weight of the first two polysaccharide distribution index(Mw/Mn)were 1.
26415455	19	43	theme	molecular	1909:1917	arg1	1					1988:1988	1	1988:1988	1	1988:1988	The molecular weight of the first two polysaccharide distribution index(Mw/Mn)were 1.
26415455	12	44	theme	HPSEC-MALLS-RI	1564:1577	arg1	system					1579:1584	HPSEC-MALLS-RI system	1564:1584	HPSEC-MALLS-RI system	1564:1584	HPSEC-MALLS-RI system showed that all the polysaccharide samples had the similar compositions.
26415455	6	45	theme	Chaenomeles	1056:1066	arg1	fruits					1077:1082	Chaenomeles speciosa fruits	1056:1082	Chaenomeles speciosa fruits	1056:1082	The aim of the study is to determine the amount and the molecular weight of the polysaccharides from Chaenomeles speciosa fruits, and preliminary investigate the immunomodulatory activity, The study provided the basis datas for the further research of Chaenomeles speciosa fruits.
26415455	12	46	contain	had	1629:1631	arg1	samples					1621:1627	all the polysaccharide samples	1598:1627	all the polysaccharide samples	1598:1627	HPSEC-MALLS-RI system showed that all the polysaccharide samples had the similar compositions.
26415455	12	46	contain	had	1629:1631	arg2	compositions					1645:1656	the similar compositions	1633:1656	the similar compositions	1633:1656	HPSEC-MALLS-RI system showed that all the polysaccharide samples had the similar compositions.
26415455	7	47	theme	polysaccharide	1155:1168	arg1	research					1135:1142	the research	1131:1142	the research of natural polysaccharide	1131:1168	, and provided a simple and system method for the research of natural polysaccharide.
26415455	9	48	theme	Chaenomeles	1340:1350	arg1	fruits					1361:1366	Chaenomeles speciosa fruits	1340:1366	Chaenomeles speciosa fruits	1340:1366	The results indicated that most polysaccharide from Chaenomeles speciosa fruits might be precipitated when ethanol concentration was up to 95% (T) and the crude polysaccharide purity had risen from 35.
26415455	0	49	theme	Amount	34:39	arg1	Investigation					13:25	[Preliminary Investigation	0:25	[Preliminary Investigation of the Amount, the Molecular Weight and the Activity of Polysaccharides from Chaenomeles Speciosa Fruits in Ethanol Fractional Precipitation	0:166	[Preliminary Investigation of the Amount, the Molecular Weight and the Activity of Polysaccharides from Chaenomeles Speciosa Fruits in Ethanol Fractional Precipitation].
26415455	22	50	theme	polysaccharide	2024:2037	arg1	samples					2039:2045	The polysaccharide samples	2020:2045	The polysaccharide samples	2020:2045	The polysaccharide samples had not exhibited immunomodulatory activity assessed on the basis of nitric oxide production by RAW264.
26415455	0	51	theme	Weight	56:61	arg1	Investigation					13:25	[Preliminary Investigation	0:25	[Preliminary Investigation of the Amount, the Molecular Weight and the Activity of Polysaccharides from Chaenomeles Speciosa Fruits in Ethanol Fractional Precipitation	0:166	[Preliminary Investigation of the Amount, the Molecular Weight and the Activity of Polysaccharides from Chaenomeles Speciosa Fruits in Ethanol Fractional Precipitation].
26415455	9	52	dep	95	1427:1428	arg1	to					1424:1425	to	1424:1425	to	1424:1425	The results indicated that most polysaccharide from Chaenomeles speciosa fruits might be precipitated when ethanol concentration was up to 95% (T) and the crude polysaccharide purity had risen from 35.
26415455	19	53	theme	first	1933:1937	arg1	Mw/Mn					1977:1981	Mw/Mn	1977:1981	Mw/Mn	1977:1981	The molecular weight of the first two polysaccharide distribution index(Mw/Mn)were 1.
26415455	19	53	theme	first	1933:1937	arg1	index					1971:1975	the first two polysaccharide distribution index	1929:1975	the first two polysaccharide distribution index(Mw/Mn)	1929:1982	The molecular weight of the first two polysaccharide distribution index(Mw/Mn)were 1.
26415455	0	54	dep	Amount	34:39	arg1	the					30:32	the	30:32	the	30:32	[Preliminary Investigation of the Amount, the Molecular Weight and the Activity of Polysaccharides from Chaenomeles Speciosa Fruits in Ethanol Fractional Precipitation].
26415455	4	55	theme	weigh	617:621	arg1	distribution					623:634	molecular weigh distribution	607:634	molecular weigh distribution	607:634	A method using high-performance size-exclusion chromatography (HPSEC) equipped with multiangle laser-light-scattering photometry (MALLS) and differential refractometry (RI) was presented for determining the molecular weight and molecular weigh distribution.
26415455	11	56	theme	20	1552:1553	arg1	%					1554:1554	%	1554:1554	%	1554:1554	0% when the concentration of ethanol increased from 20% to 95%.
26415455	6	57	theme	speciosa	917:924	arg1	fruits					926:931	Chaenomeles speciosa fruits	905:931	Chaenomeles speciosa fruits	905:931	The aim of the study is to determine the amount and the molecular weight of the polysaccharides from Chaenomeles speciosa fruits, and preliminary investigate the immunomodulatory activity, The study provided the basis datas for the further research of Chaenomeles speciosa fruits.
26415455	4	58	theme	molecular	586:594	arg1	weight					596:601	the molecular weight	582:601	the molecular weight	582:601	A method using high-performance size-exclusion chromatography (HPSEC) equipped with multiangle laser-light-scattering photometry (MALLS) and differential refractometry (RI) was presented for determining the molecular weight and molecular weigh distribution.
26415455	7	59	theme	system	1113:1118	arg1	method					1120:1125	a simple and system method	1100:1125	a simple and system method for the research of natural polysaccharide	1100:1168	, and provided a simple and system method for the research of natural polysaccharide.
26415455	6	60	theme	study	819:823	arg1	aim					808:810	The aim	804:810	The aim of the study	804:823	The aim of the study is to determine the amount and the molecular weight of the polysaccharides from Chaenomeles speciosa fruits, and preliminary investigate the immunomodulatory activity, The study provided the basis datas for the further research of Chaenomeles speciosa fruits.
26415455	5	61	from	production	728:737	arg1	cells					762:766	the cells	758:766	the cells	758:766	RAW264.7 macrophage were cultured and stimulated with the polysaccharides in vitro and the production of nitric oxide in the cells was determined by the Griess assay.
26415455	4	62	theme	high-performance	394:409	arg1	HPSEC					442:446	HPSEC	442:446	HPSEC	442:446	A method using high-performance size-exclusion chromatography (HPSEC) equipped with multiangle laser-light-scattering photometry (MALLS) and differential refractometry (RI) was presented for determining the molecular weight and molecular weigh distribution.
26415455	4	62	theme	high-performance	394:409	arg1	chromatography					426:439	high-performance size-exclusion chromatography	394:439	high-performance size-exclusion chromatography (HPSEC) equipped with multiangle laser-light-scattering photometry (MALLS) and differential refractometry (RI)	394:550	A method using high-performance size-exclusion chromatography (HPSEC) equipped with multiangle laser-light-scattering photometry (MALLS) and differential refractometry (RI) was presented for determining the molecular weight and molecular weigh distribution.
26415455	11	63	dep	increased	1537:1545	arg1	%					1501:1501	%	1501:1501	%	1501:1501	0% when the concentration of ethanol increased from 20% to 95%.
26415455	7	64	theme	simple	1102:1107	arg1	method					1120:1125	a simple and system method	1100:1125	a simple and system method for the research of natural polysaccharide	1100:1168	, and provided a simple and system method for the research of natural polysaccharide.
26415455	1	65	theme	Chaenomeles	170:180	arg1	fruits					191:196	Chaenomeles speciosa fruits	170:196	Chaenomeles speciosa fruits	170:196	Chaenomeles speciosa fruits were extracted using water.
26415455	8	66	theme	ethanol	1175:1181	arg1	precipitation					1194:1206	The ethanol fractional precipitation	1171:1206	The ethanol fractional precipitation	1171:1206	The ethanol fractional precipitation showed that the order of total polysaccharide content was 95%>80%>40% ≥60%>20%.
26415455	18	67	theme	accurate	1888:1895	arg1	values					1897:1902	accurate values	1888:1902	accurate values	1888:1902	mol-1 respectively, and one less than 10 000 which was failure to obtain accurate values.
26415455	8	68	theme	content	1254:1260	arg1	%					1285:1285	95%>80%>40% ≥60%>20%	1266:1285	95%>80%>40% ≥60%>20%	1266:1285	The ethanol fractional precipitation showed that the order of total polysaccharide content was 95%>80%>40% ≥60%>20%.
26415455	8	68	theme	content	1254:1260	arg1	order					1224:1228	the order	1220:1228	the order of total polysaccharide content	1220:1260	The ethanol fractional precipitation showed that the order of total polysaccharide content was 95%>80%>40% ≥60%>20%.
26415455	0	69	from	Investigation	13:25	arg1	Precipitation					154:166	Ethanol Fractional Precipitation	135:166	Ethanol Fractional Precipitation	135:166	[Preliminary Investigation of the Amount, the Molecular Weight and the Activity of Polysaccharides from Chaenomeles Speciosa Fruits in Ethanol Fractional Precipitation].
26415455	6	70	from	weight	870:875	arg1	fruits					926:931	Chaenomeles speciosa fruits	905:931	Chaenomeles speciosa fruits	905:931	The aim of the study is to determine the amount and the molecular weight of the polysaccharides from Chaenomeles speciosa fruits, and preliminary investigate the immunomodulatory activity, The study provided the basis datas for the further research of Chaenomeles speciosa fruits.
26415455	22	71	theme	nitric	2116:2121	arg1	oxide					2123:2127	nitric oxide	2116:2127	nitric oxide production	2116:2138	The polysaccharide samples had not exhibited immunomodulatory activity assessed on the basis of nitric oxide production by RAW264.
26415455	2	72	with	precipitated	244:255	arg1	ethanol					274:280	20%~95% (φ) ethanol	262:280	20%~95% (φ) ethanol	262:280	The extracts were precipitated with 20%~95% (φ) ethanol, respectively.
26415455	0	73	from	Activity	71:78	arg1	Precipitation					154:166	Ethanol Fractional Precipitation	135:166	Ethanol Fractional Precipitation	135:166	[Preliminary Investigation of the Amount, the Molecular Weight and the Activity of Polysaccharides from Chaenomeles Speciosa Fruits in Ethanol Fractional Precipitation].
26415455	0	73	from	Activity	71:78	arg1	Fruits					125:130	Chaenomeles Speciosa Fruits	104:130	Chaenomeles Speciosa Fruits	104:130	[Preliminary Investigation of the Amount, the Molecular Weight and the Activity of Polysaccharides from Chaenomeles Speciosa Fruits in Ethanol Fractional Precipitation].
26415455	11	74	dep	%	1554:1554	arg1	to					1556:1557	to	1556:1557	to	1556:1557	0% when the concentration of ethanol increased from 20% to 95%.
26415455	5	75	theme	nitric	742:747	arg1	oxide					749:753	nitric oxide	742:753	nitric oxide	742:753	RAW264.7 macrophage were cultured and stimulated with the polysaccharides in vitro and the production of nitric oxide in the cells was determined by the Griess assay.
26415455	4	76	theme	multiangle	463:472	arg1	MALLS					509:513	MALLS	509:513	MALLS	509:513	A method using high-performance size-exclusion chromatography (HPSEC) equipped with multiangle laser-light-scattering photometry (MALLS) and differential refractometry (RI) was presented for determining the molecular weight and molecular weigh distribution.
26415455	4	76	theme	multiangle	463:472	arg1	photometry					497:506	multiangle laser-light-scattering photometry	463:506	multiangle laser-light-scattering photometry (MALLS)	463:514	A method using high-performance size-exclusion chromatography (HPSEC) equipped with multiangle laser-light-scattering photometry (MALLS) and differential refractometry (RI) was presented for determining the molecular weight and molecular weigh distribution.
26415455	8	77	theme	>40	1273:1275	arg1	%					1281:1281	95%>80%>40% ≥60%	1266:1281	95%>80%>40% ≥60%>20%	1266:1285	The ethanol fractional precipitation showed that the order of total polysaccharide content was 95%>80%>40% ≥60%>20%.
26415455	6	78	from	amount	845:850	arg1	fruits					926:931	Chaenomeles speciosa fruits	905:931	Chaenomeles speciosa fruits	905:931	The aim of the study is to determine the amount and the molecular weight of the polysaccharides from Chaenomeles speciosa fruits, and preliminary investigate the immunomodulatory activity, The study provided the basis datas for the further research of Chaenomeles speciosa fruits.
26415455	9	79	theme	polysaccharide	1449:1462	arg1	purity					1464:1469	the crude polysaccharide purity	1439:1469	the crude polysaccharide purity	1439:1469	The results indicated that most polysaccharide from Chaenomeles speciosa fruits might be precipitated when ethanol concentration was up to 95% (T) and the crude polysaccharide purity had risen from 35.
26415455	8	80	theme	total	1233:1237	arg1	content					1254:1260	total polysaccharide content	1233:1260	total polysaccharide content	1233:1260	The ethanol fractional precipitation showed that the order of total polysaccharide content was 95%>80%>40% ≥60%>20%.
26415455	6	81	theme	further	1036:1042	arg1	research					1044:1051	the further research	1032:1051	the further research of Chaenomeles speciosa fruits	1032:1082	The aim of the study is to determine the amount and the molecular weight of the polysaccharides from Chaenomeles speciosa fruits, and preliminary investigate the immunomodulatory activity, The study provided the basis datas for the further research of Chaenomeles speciosa fruits.
26415455	3	82	theme	total	311:315	arg1	polysaccharide					317:330	total polysaccharide	311:330	total polysaccharide	311:330	The amount of total polysaccharide was measured with phenol-sulfuric acid method.
26415455	0	83	theme	Activity	71:78	arg1	Investigation					13:25	[Preliminary Investigation	0:25	[Preliminary Investigation of the Amount, the Molecular Weight and the Activity of Polysaccharides from Chaenomeles Speciosa Fruits in Ethanol Fractional Precipitation	0:166	[Preliminary Investigation of the Amount, the Molecular Weight and the Activity of Polysaccharides from Chaenomeles Speciosa Fruits in Ethanol Fractional Precipitation].
26415455	9	84	from	fruits	1361:1366	arg1	polysaccharide					1320:1333	most polysaccharide	1315:1333	most polysaccharide from Chaenomeles speciosa fruits	1315:1366	The results indicated that most polysaccharide from Chaenomeles speciosa fruits might be precipitated when ethanol concentration was up to 95% (T) and the crude polysaccharide purity had risen from 35.
26415455	6	85	dep	basis	1016:1020	arg1	datas					1022:1026	datas	1022:1026	the basis datas for the further research of Chaenomeles speciosa fruits	1012:1082	The aim of the study is to determine the amount and the molecular weight of the polysaccharides from Chaenomeles speciosa fruits, and preliminary investigate the immunomodulatory activity, The study provided the basis datas for the further research of Chaenomeles speciosa fruits.
26415455	0	86	theme	Polysaccharides	83:97	arg1	Polysaccharides					83:97	Polysaccharides	83:97	Polysaccharides from Chaenomeles Speciosa Fruits	83:130	[Preliminary Investigation of the Amount, the Molecular Weight and the Activity of Polysaccharides from Chaenomeles Speciosa Fruits in Ethanol Fractional Precipitation].
26415455	0	86	theme	Polysaccharides	83:97	arg1	Weight					56:61	the Molecular Weight	42:61	the Molecular Weight	42:61	[Preliminary Investigation of the Amount, the Molecular Weight and the Activity of Polysaccharides from Chaenomeles Speciosa Fruits in Ethanol Fractional Precipitation].
26415455	0	86	theme	Polysaccharides	83:97	arg1	Activity					71:78	the Activity	67:78	the Activity of Polysaccharides from Chaenomeles Speciosa Fruits	67:130	[Preliminary Investigation of the Amount, the Molecular Weight and the Activity of Polysaccharides from Chaenomeles Speciosa Fruits in Ethanol Fractional Precipitation].
26415455	0	86	theme	Polysaccharides	83:97	arg1	Amount					34:39	Amount	34:39	Amount	34:39	[Preliminary Investigation of the Amount, the Molecular Weight and the Activity of Polysaccharides from Chaenomeles Speciosa Fruits in Ethanol Fractional Precipitation].
26415455	0	87	theme	Chaenomeles	104:114	arg1	Fruits					125:130	Chaenomeles Speciosa Fruits	104:130	Chaenomeles Speciosa Fruits	104:130	[Preliminary Investigation of the Amount, the Molecular Weight and the Activity of Polysaccharides from Chaenomeles Speciosa Fruits in Ethanol Fractional Precipitation].
26415455	5	88	theme	RAW264.7	637:644	arg1	macrophage					646:655	RAW264.7 macrophage	637:655	RAW264.7 macrophage	637:655	RAW264.7 macrophage were cultured and stimulated with the polysaccharides in vitro and the production of nitric oxide in the cells was determined by the Griess assay.
26415455	0	89	theme	Ethanol	135:141	arg1	Precipitation					154:166	Ethanol Fractional Precipitation	135:166	Ethanol Fractional Precipitation	135:166	[Preliminary Investigation of the Amount, the Molecular Weight and the Activity of Polysaccharides from Chaenomeles Speciosa Fruits in Ethanol Fractional Precipitation].
26415455	9	90	theme	most	1315:1318	arg1	polysaccharide					1320:1333	most polysaccharide	1315:1333	most polysaccharide from Chaenomeles speciosa fruits	1315:1366	The results indicated that most polysaccharide from Chaenomeles speciosa fruits might be precipitated when ethanol concentration was up to 95% (T) and the crude polysaccharide purity had risen from 35.
26415455	23	91	theme	macrophage	2153:2162	arg1	cells					2164:2168	7 macrophage cells	2151:2168	7 macrophage cells in the experiment	2151:2186	7 macrophage cells in the experiment.
26415455	6	92	theme	speciosa	1068:1075	arg1	fruits					1077:1082	Chaenomeles speciosa fruits	1056:1082	Chaenomeles speciosa fruits	1056:1082	The aim of the study is to determine the amount and the molecular weight of the polysaccharides from Chaenomeles speciosa fruits, and preliminary investigate the immunomodulatory activity, The study provided the basis datas for the further research of Chaenomeles speciosa fruits.
26415455	3	93	theme	acid	366:369	arg1	method					371:376	phenol-sulfuric acid method	350:376	phenol-sulfuric acid method	350:376	The amount of total polysaccharide was measured with phenol-sulfuric acid method.
26415455	6	94	dep	amount	845:850	arg1	the					841:843	the	841:843	the	841:843	The aim of the study is to determine the amount and the molecular weight of the polysaccharides from Chaenomeles speciosa fruits, and preliminary investigate the immunomodulatory activity, The study provided the basis datas for the further research of Chaenomeles speciosa fruits.
26415455	7	95	theme	natural	1147:1153	arg1	polysaccharide					1155:1168	natural polysaccharide	1147:1168	natural polysaccharide	1147:1168	, and provided a simple and system method for the research of natural polysaccharide.
26415455	6	96	theme	molecular	860:868	arg1	weight					870:875	the molecular weight	856:875	the molecular weight	856:875	The aim of the study is to determine the amount and the molecular weight of the polysaccharides from Chaenomeles speciosa fruits, and preliminary investigate the immunomodulatory activity, The study provided the basis datas for the further research of Chaenomeles speciosa fruits.
26415455	11	97	theme	ethanol	1529:1535	arg1	concentration					1512:1524	the concentration	1508:1524	the concentration of ethanol	1508:1535	0% when the concentration of ethanol increased from 20% to 95%.
26415455	0	98	theme	Molecular	46:54	arg1	Weight					56:61	the Molecular Weight	42:61	the Molecular Weight	42:61	[Preliminary Investigation of the Amount, the Molecular Weight and the Activity of Polysaccharides from Chaenomeles Speciosa Fruits in Ethanol Fractional Precipitation].
26415455	9	99	theme	speciosa	1352:1359	arg1	fruits					1361:1366	Chaenomeles speciosa fruits	1340:1366	Chaenomeles speciosa fruits	1340:1366	The results indicated that most polysaccharide from Chaenomeles speciosa fruits might be precipitated when ethanol concentration was up to 95% (T) and the crude polysaccharide purity had risen from 35.
26415455	23	100	from	cells	2164:2168	arg1	experiment					2177:2186	the experiment	2173:2186	the experiment	2173:2186	7 macrophage cells in the experiment.
26415455	17	101	dep	393	1799:1801	arg1	4					1808:1808	4	1808:1808	4	1808:1808	393 X 10(4) g .
26415455	0	102	from	Fruits	125:130	arg1	Polysaccharides					83:97	Polysaccharides	83:97	Polysaccharides from Chaenomeles Speciosa Fruits	83:130	[Preliminary Investigation of the Amount, the Molecular Weight and the Activity of Polysaccharides from Chaenomeles Speciosa Fruits in Ethanol Fractional Precipitation].
26415455	0	102	from	Fruits	125:130	arg1	Weight					56:61	the Molecular Weight	42:61	the Molecular Weight	42:61	[Preliminary Investigation of the Amount, the Molecular Weight and the Activity of Polysaccharides from Chaenomeles Speciosa Fruits in Ethanol Fractional Precipitation].
26415455	0	102	from	Fruits	125:130	arg1	Activity					71:78	the Activity	67:78	the Activity of Polysaccharides from Chaenomeles Speciosa Fruits	67:130	[Preliminary Investigation of the Amount, the Molecular Weight and the Activity of Polysaccharides from Chaenomeles Speciosa Fruits in Ethanol Fractional Precipitation].
26415455	0	102	from	Fruits	125:130	arg1	Amount					34:39	Amount	34:39	Amount	34:39	[Preliminary Investigation of the Amount, the Molecular Weight and the Activity of Polysaccharides from Chaenomeles Speciosa Fruits in Ethanol Fractional Precipitation].
26415455	9	103	theme	ethanol	1395:1401	arg1	concentration					1403:1415	ethanol concentration	1395:1415	ethanol concentration	1395:1415	The results indicated that most polysaccharide from Chaenomeles speciosa fruits might be precipitated when ethanol concentration was up to 95% (T) and the crude polysaccharide purity had risen from 35.
25151069	1	0	theme	fixed-bed	298:306	arg1	reactor					308:314	a fixed-bed reactor	296:314	a fixed-bed reactor	296:314	The pyrolysis characteristics of xylan, cellulose, ADF (a mixture of cellulose and lignin extracted from pine wood) and pine wood were investigated in a fixed-bed reactor by determining the distributions of three-phase products, the elemental compositions of char products, the conversions of components and the profiles of gas release rate during pyrolysis as well as the compositions of liquid products.
25151069	1	1	theme	pyrolysis	149:157	arg1	characteristics					159:173	The pyrolysis characteristics	145:173	The pyrolysis characteristics of xylan, cellulose, ADF (a mixture of cellulose and lignin extracted from pine wood) and pine wood	145:273	The pyrolysis characteristics of xylan, cellulose, ADF (a mixture of cellulose and lignin extracted from pine wood) and pine wood were investigated in a fixed-bed reactor by determining the distributions of three-phase products, the elemental compositions of char products, the conversions of components and the profiles of gas release rate during pyrolysis as well as the compositions of liquid products.
25151069	1	2	theme	lignin	228:233	arg1	mixture					203:209	a mixture	201:209	a mixture of cellulose and lignin extracted from pine wood	201:258	The pyrolysis characteristics of xylan, cellulose, ADF (a mixture of cellulose and lignin extracted from pine wood) and pine wood were investigated in a fixed-bed reactor by determining the distributions of three-phase products, the elemental compositions of char products, the conversions of components and the profiles of gas release rate during pyrolysis as well as the compositions of liquid products.
25151069	4	3	theme	componential	795:806	arg1	interactions					808:819	the componential interactions	791:819	the componential interactions	791:819	Pine wood exhibited the componential interactions, resulting in the broadened temperature range of mass loss, the enhanced yield of char, and the increased heterogeneity of char.
25151069	4	4	theme	temperature	849:859	arg1	range					861:865	the broadened temperature range	835:865	the broadened temperature range of mass loss	835:878	Pine wood exhibited the componential interactions, resulting in the broadened temperature range of mass loss, the enhanced yield of char, and the increased heterogeneity of char.
25151069	1	5	theme	products	409:416	arg1	compositions					388:399	the elemental compositions	374:399	the elemental compositions of char products	374:416	The pyrolysis characteristics of xylan, cellulose, ADF (a mixture of cellulose and lignin extracted from pine wood) and pine wood were investigated in a fixed-bed reactor by determining the distributions of three-phase products, the elemental compositions of char products, the conversions of components and the profiles of gas release rate during pyrolysis as well as the compositions of liquid products.
25151069	1	5	theme	products	409:416	arg1	profiles					457:464	the profiles	453:464	the profiles of gas release rate	453:484	The pyrolysis characteristics of xylan, cellulose, ADF (a mixture of cellulose and lignin extracted from pine wood) and pine wood were investigated in a fixed-bed reactor by determining the distributions of three-phase products, the elemental compositions of char products, the conversions of components and the profiles of gas release rate during pyrolysis as well as the compositions of liquid products.
25151069	1	5	theme	products	409:416	arg1	conversions					423:433	the conversions	419:433	the conversions of components	419:447	The pyrolysis characteristics of xylan, cellulose, ADF (a mixture of cellulose and lignin extracted from pine wood) and pine wood were investigated in a fixed-bed reactor by determining the distributions of three-phase products, the elemental compositions of char products, the conversions of components and the profiles of gas release rate during pyrolysis as well as the compositions of liquid products.
25151069	1	5	theme	products	409:416	arg1	distributions					335:347	the distributions	331:347	the distributions of three-phase products	331:371	The pyrolysis characteristics of xylan, cellulose, ADF (a mixture of cellulose and lignin extracted from pine wood) and pine wood were investigated in a fixed-bed reactor by determining the distributions of three-phase products, the elemental compositions of char products, the conversions of components and the profiles of gas release rate during pyrolysis as well as the compositions of liquid products.
25151069	3	6	theme	CO	660:661	arg1	release					641:647	the release	637:647	the release of CO2 and CO from cellulose	637:676	Lignin accelerated the release of CO2 and CO from cellulose and intensified the decomposition of cellulose to smaller molecular weight liquid compounds.
25151069	4	7	theme	mass	870:873	arg1	loss					875:878	mass loss	870:878	mass loss	870:878	Pine wood exhibited the componential interactions, resulting in the broadened temperature range of mass loss, the enhanced yield of char, and the increased heterogeneity of char.
25151069	6	8	from	formation	1061:1069	arg1	wood					1101:1104	pine wood	1096:1104	pine wood	1096:1104	The formation of liquid compounds from pine wood was also observed to be influenced by the componential interactions.
25151069	1	9	theme	pine	250:253	arg1	wood					255:258	pine wood	250:258	pine wood	250:258	The pyrolysis characteristics of xylan, cellulose, ADF (a mixture of cellulose and lignin extracted from pine wood) and pine wood were investigated in a fixed-bed reactor by determining the distributions of three-phase products, the elemental compositions of char products, the conversions of components and the profiles of gas release rate during pyrolysis as well as the compositions of liquid products.
25151069	2	10	theme	different	596:604	arg1	components					606:615	the different components	592:615	the different components	592:615	Interactions were found to occur between the different components.
25151069	4	11	theme	increased	917:925	arg1	heterogeneity					927:939	the increased heterogeneity	913:939	the increased heterogeneity of char	913:947	Pine wood exhibited the componential interactions, resulting in the broadened temperature range of mass loss, the enhanced yield of char, and the increased heterogeneity of char.
25151069	5	12	theme	Pine	950:953	arg1	wood					955:958	Pine wood	950:958	Pine wood	950:958	Pine wood produced more bio-oil than each component sample, with the compositional formula of CH1.07O0.31.
25151069	1	13	theme	xylan	178:182	arg1	characteristics					159:173	The pyrolysis characteristics	145:173	The pyrolysis characteristics of xylan, cellulose, ADF (a mixture of cellulose and lignin extracted from pine wood) and pine wood	145:273	The pyrolysis characteristics of xylan, cellulose, ADF (a mixture of cellulose and lignin extracted from pine wood) and pine wood were investigated in a fixed-bed reactor by determining the distributions of three-phase products, the elemental compositions of char products, the conversions of components and the profiles of gas release rate during pyrolysis as well as the compositions of liquid products.
25151069	0	14	theme	comparative	2:12	arg1	investigation					14:26	A comparative investigation	0:26	A comparative investigation into the formation behaviors of char	0:63	A comparative investigation into the formation behaviors of char, liquids and gases during pyrolysis of pinewood and lignocellulosic components.
25151069	0	14	theme	comparative	2:12	arg1	liquids					66:72	liquids	66:72	liquids	66:72	A comparative investigation into the formation behaviors of char, liquids and gases during pyrolysis of pinewood and lignocellulosic components.
25151069	0	14	theme	comparative	2:12	arg1	gases					78:82	gases	78:82	gases	78:82	A comparative investigation into the formation behaviors of char, liquids and gases during pyrolysis of pinewood and lignocellulosic components.
25151069	3	15	theme	cellulose	715:723	arg1	decomposition					698:710	the decomposition	694:710	the decomposition of cellulose to smaller molecular weight liquid compounds	694:768	Lignin accelerated the release of CO2 and CO from cellulose and intensified the decomposition of cellulose to smaller molecular weight liquid compounds.
25151069	4	16	theme	broadened	839:847	arg1	range					861:865	the broadened temperature range	835:865	the broadened temperature range of mass loss	835:878	Pine wood exhibited the componential interactions, resulting in the broadened temperature range of mass loss, the enhanced yield of char, and the increased heterogeneity of char.
25151069	1	17	theme	components	438:447	arg1	compositions					388:399	the elemental compositions	374:399	the elemental compositions of char products	374:416	The pyrolysis characteristics of xylan, cellulose, ADF (a mixture of cellulose and lignin extracted from pine wood) and pine wood were investigated in a fixed-bed reactor by determining the distributions of three-phase products, the elemental compositions of char products, the conversions of components and the profiles of gas release rate during pyrolysis as well as the compositions of liquid products.
25151069	1	17	theme	components	438:447	arg1	profiles					457:464	the profiles	453:464	the profiles of gas release rate	453:484	The pyrolysis characteristics of xylan, cellulose, ADF (a mixture of cellulose and lignin extracted from pine wood) and pine wood were investigated in a fixed-bed reactor by determining the distributions of three-phase products, the elemental compositions of char products, the conversions of components and the profiles of gas release rate during pyrolysis as well as the compositions of liquid products.
25151069	1	17	theme	components	438:447	arg1	conversions					423:433	the conversions	419:433	the conversions of components	419:447	The pyrolysis characteristics of xylan, cellulose, ADF (a mixture of cellulose and lignin extracted from pine wood) and pine wood were investigated in a fixed-bed reactor by determining the distributions of three-phase products, the elemental compositions of char products, the conversions of components and the profiles of gas release rate during pyrolysis as well as the compositions of liquid products.
25151069	1	17	theme	components	438:447	arg1	distributions					335:347	the distributions	331:347	the distributions of three-phase products	331:371	The pyrolysis characteristics of xylan, cellulose, ADF (a mixture of cellulose and lignin extracted from pine wood) and pine wood were investigated in a fixed-bed reactor by determining the distributions of three-phase products, the elemental compositions of char products, the conversions of components and the profiles of gas release rate during pyrolysis as well as the compositions of liquid products.
25151069	5	18	theme	compositional	1019:1031	arg1	formula					1033:1039	the compositional formula	1015:1039	the compositional formula of CH1.07O0.31	1015:1054	Pine wood produced more bio-oil than each component sample, with the compositional formula of CH1.07O0.31.
25151069	1	19	theme	cellulose	185:193	arg1	characteristics					159:173	The pyrolysis characteristics	145:173	The pyrolysis characteristics of xylan, cellulose, ADF (a mixture of cellulose and lignin extracted from pine wood) and pine wood	145:273	The pyrolysis characteristics of xylan, cellulose, ADF (a mixture of cellulose and lignin extracted from pine wood) and pine wood were investigated in a fixed-bed reactor by determining the distributions of three-phase products, the elemental compositions of char products, the conversions of components and the profiles of gas release rate during pyrolysis as well as the compositions of liquid products.
25151069	3	20	theme	smaller	728:734	arg1	compounds					760:768	smaller molecular weight liquid compounds	728:768	smaller molecular weight liquid compounds	728:768	Lignin accelerated the release of CO2 and CO from cellulose and intensified the decomposition of cellulose to smaller molecular weight liquid compounds.
25151069	1	21	theme	liquid	534:539	arg1	products					541:548	liquid products	534:548	liquid products	534:548	The pyrolysis characteristics of xylan, cellulose, ADF (a mixture of cellulose and lignin extracted from pine wood) and pine wood were investigated in a fixed-bed reactor by determining the distributions of three-phase products, the elemental compositions of char products, the conversions of components and the profiles of gas release rate during pyrolysis as well as the compositions of liquid products.
25151069	0	22	theme	pinewood	104:111	arg1	components					133:142	pinewood and lignocellulosic components	104:142	pinewood and lignocellulosic components	104:142	A comparative investigation into the formation behaviors of char, liquids and gases during pyrolysis of pinewood and lignocellulosic components.
25151069	1	23	theme	three-phase	352:362	arg1	products					364:371	three-phase products	352:371	three-phase products	352:371	The pyrolysis characteristics of xylan, cellulose, ADF (a mixture of cellulose and lignin extracted from pine wood) and pine wood were investigated in a fixed-bed reactor by determining the distributions of three-phase products, the elemental compositions of char products, the conversions of components and the profiles of gas release rate during pyrolysis as well as the compositions of liquid products.
25151069	4	24	gly	heterogeneity	927:939	arg1	char					944:947	char	944:947	char	944:947	Pine wood exhibited the componential interactions, resulting in the broadened temperature range of mass loss, the enhanced yield of char, and the increased heterogeneity of char.
25151069	4	24	gly	heterogeneity	927:939	arg1	loss					875:878	mass loss	870:878	mass loss	870:878	Pine wood exhibited the componential interactions, resulting in the broadened temperature range of mass loss, the enhanced yield of char, and the increased heterogeneity of char.
25151069	4	24	gly	heterogeneity	927:939	arg1	char					903:906	char	903:906	char	903:906	Pine wood exhibited the componential interactions, resulting in the broadened temperature range of mass loss, the enhanced yield of char, and the increased heterogeneity of char.
25151069	1	25	theme	products	541:548	arg1	compositions					518:529	the compositions	514:529	pyrolysis as well as the compositions of liquid products	493:548	The pyrolysis characteristics of xylan, cellulose, ADF (a mixture of cellulose and lignin extracted from pine wood) and pine wood were investigated in a fixed-bed reactor by determining the distributions of three-phase products, the elemental compositions of char products, the conversions of components and the profiles of gas release rate during pyrolysis as well as the compositions of liquid products.
25151069	1	25	theme	products	541:548	arg1	pyrolysis					493:501	pyrolysis	493:501	pyrolysis as well as the compositions of liquid products	493:548	The pyrolysis characteristics of xylan, cellulose, ADF (a mixture of cellulose and lignin extracted from pine wood) and pine wood were investigated in a fixed-bed reactor by determining the distributions of three-phase products, the elemental compositions of char products, the conversions of components and the profiles of gas release rate during pyrolysis as well as the compositions of liquid products.
25151069	1	26	theme	ADF	196:198	arg1	characteristics					159:173	The pyrolysis characteristics	145:173	The pyrolysis characteristics of xylan, cellulose, ADF (a mixture of cellulose and lignin extracted from pine wood) and pine wood	145:273	The pyrolysis characteristics of xylan, cellulose, ADF (a mixture of cellulose and lignin extracted from pine wood) and pine wood were investigated in a fixed-bed reactor by determining the distributions of three-phase products, the elemental compositions of char products, the conversions of components and the profiles of gas release rate during pyrolysis as well as the compositions of liquid products.
25151069	0	27	theme	formation	37:45	arg1	behaviors					47:55	the formation behaviors	33:55	the formation behaviors of char	33:63	A comparative investigation into the formation behaviors of char, liquids and gases during pyrolysis of pinewood and lignocellulosic components.
25151069	1	28	theme	pine	265:268	arg1	wood					270:273	pine wood	265:273	pine wood	265:273	The pyrolysis characteristics of xylan, cellulose, ADF (a mixture of cellulose and lignin extracted from pine wood) and pine wood were investigated in a fixed-bed reactor by determining the distributions of three-phase products, the elemental compositions of char products, the conversions of components and the profiles of gas release rate during pyrolysis as well as the compositions of liquid products.
25151069	0	29	theme	components	133:142	arg1	pyrolysis					91:99	pyrolysis	91:99	pyrolysis of pinewood and lignocellulosic components	91:142	A comparative investigation into the formation behaviors of char, liquids and gases during pyrolysis of pinewood and lignocellulosic components.
25151069	1	30	theme	products	364:371	arg1	compositions					388:399	the elemental compositions	374:399	the elemental compositions of char products	374:416	The pyrolysis characteristics of xylan, cellulose, ADF (a mixture of cellulose and lignin extracted from pine wood) and pine wood were investigated in a fixed-bed reactor by determining the distributions of three-phase products, the elemental compositions of char products, the conversions of components and the profiles of gas release rate during pyrolysis as well as the compositions of liquid products.
25151069	1	30	theme	products	364:371	arg1	profiles					457:464	the profiles	453:464	the profiles of gas release rate	453:484	The pyrolysis characteristics of xylan, cellulose, ADF (a mixture of cellulose and lignin extracted from pine wood) and pine wood were investigated in a fixed-bed reactor by determining the distributions of three-phase products, the elemental compositions of char products, the conversions of components and the profiles of gas release rate during pyrolysis as well as the compositions of liquid products.
25151069	1	30	theme	products	364:371	arg1	conversions					423:433	the conversions	419:433	the conversions of components	419:447	The pyrolysis characteristics of xylan, cellulose, ADF (a mixture of cellulose and lignin extracted from pine wood) and pine wood were investigated in a fixed-bed reactor by determining the distributions of three-phase products, the elemental compositions of char products, the conversions of components and the profiles of gas release rate during pyrolysis as well as the compositions of liquid products.
25151069	1	30	theme	products	364:371	arg1	distributions					335:347	the distributions	331:347	the distributions of three-phase products	331:371	The pyrolysis characteristics of xylan, cellulose, ADF (a mixture of cellulose and lignin extracted from pine wood) and pine wood were investigated in a fixed-bed reactor by determining the distributions of three-phase products, the elemental compositions of char products, the conversions of components and the profiles of gas release rate during pyrolysis as well as the compositions of liquid products.
25151069	4	31	theme	char	903:906	arg1	range					861:865	the broadened temperature range	835:865	the broadened temperature range of mass loss	835:878	Pine wood exhibited the componential interactions, resulting in the broadened temperature range of mass loss, the enhanced yield of char, and the increased heterogeneity of char.
25151069	4	31	theme	char	903:906	arg1	heterogeneity					927:939	the increased heterogeneity	913:939	the increased heterogeneity of char	913:947	Pine wood exhibited the componential interactions, resulting in the broadened temperature range of mass loss, the enhanced yield of char, and the increased heterogeneity of char.
25151069	4	31	theme	char	903:906	arg1	yield					894:898	the enhanced yield	881:898	the enhanced yield of char	881:906	Pine wood exhibited the componential interactions, resulting in the broadened temperature range of mass loss, the enhanced yield of char, and the increased heterogeneity of char.
25151069	6	32	theme	componential	1148:1159	arg1	interactions					1161:1172	the componential interactions	1144:1172	the componential interactions	1144:1172	The formation of liquid compounds from pine wood was also observed to be influenced by the componential interactions.
25151069	1	33	theme	wood	270:273	arg1	characteristics					159:173	The pyrolysis characteristics	145:173	The pyrolysis characteristics of xylan, cellulose, ADF (a mixture of cellulose and lignin extracted from pine wood) and pine wood	145:273	The pyrolysis characteristics of xylan, cellulose, ADF (a mixture of cellulose and lignin extracted from pine wood) and pine wood were investigated in a fixed-bed reactor by determining the distributions of three-phase products, the elemental compositions of char products, the conversions of components and the profiles of gas release rate during pyrolysis as well as the compositions of liquid products.
25151069	0	34	theme	lignocellulosic	117:131	arg1	components					133:142	pinewood and lignocellulosic components	104:142	pinewood and lignocellulosic components	104:142	A comparative investigation into the formation behaviors of char, liquids and gases during pyrolysis of pinewood and lignocellulosic components.
25151069	5	35	theme	CH1.07O0.31	1044:1054	arg1	formula					1033:1039	the compositional formula	1015:1039	the compositional formula of CH1.07O0.31	1015:1054	Pine wood produced more bio-oil than each component sample, with the compositional formula of CH1.07O0.31.
25151069	3	36	theme	liquid	753:758	arg1	compounds					760:768	smaller molecular weight liquid compounds	728:768	smaller molecular weight liquid compounds	728:768	Lignin accelerated the release of CO2 and CO from cellulose and intensified the decomposition of cellulose to smaller molecular weight liquid compounds.
25151069	5	37	theme	component	992:1000	arg1	sample					1002:1007	each component sample	987:1007	each component sample	987:1007	Pine wood produced more bio-oil than each component sample, with the compositional formula of CH1.07O0.31.
25151069	3	38	from	cellulose	668:676	arg1	release					641:647	the release	637:647	the release of CO2 and CO from cellulose	637:676	Lignin accelerated the release of CO2 and CO from cellulose and intensified the decomposition of cellulose to smaller molecular weight liquid compounds.
25151069	3	39	theme	molecular	736:744	arg1	compounds					760:768	smaller molecular weight liquid compounds	728:768	smaller molecular weight liquid compounds	728:768	Lignin accelerated the release of CO2 and CO from cellulose and intensified the decomposition of cellulose to smaller molecular weight liquid compounds.
25151069	4	40	theme	loss	875:878	arg1	range					861:865	the broadened temperature range	835:865	the broadened temperature range of mass loss	835:878	Pine wood exhibited the componential interactions, resulting in the broadened temperature range of mass loss, the enhanced yield of char, and the increased heterogeneity of char.
25151069	4	40	theme	loss	875:878	arg1	heterogeneity					927:939	the increased heterogeneity	913:939	the increased heterogeneity of char	913:947	Pine wood exhibited the componential interactions, resulting in the broadened temperature range of mass loss, the enhanced yield of char, and the increased heterogeneity of char.
25151069	4	40	theme	loss	875:878	arg1	yield					894:898	the enhanced yield	881:898	the enhanced yield of char	881:906	Pine wood exhibited the componential interactions, resulting in the broadened temperature range of mass loss, the enhanced yield of char, and the increased heterogeneity of char.
25151069	1	41	theme	elemental	378:386	arg1	compositions					388:399	the elemental compositions	374:399	the elemental compositions of char products	374:416	The pyrolysis characteristics of xylan, cellulose, ADF (a mixture of cellulose and lignin extracted from pine wood) and pine wood were investigated in a fixed-bed reactor by determining the distributions of three-phase products, the elemental compositions of char products, the conversions of components and the profiles of gas release rate during pyrolysis as well as the compositions of liquid products.
25151069	1	42	theme	gas	469:471	arg1	rate					481:484	gas release rate	469:484	gas release rate	469:484	The pyrolysis characteristics of xylan, cellulose, ADF (a mixture of cellulose and lignin extracted from pine wood) and pine wood were investigated in a fixed-bed reactor by determining the distributions of three-phase products, the elemental compositions of char products, the conversions of components and the profiles of gas release rate during pyrolysis as well as the compositions of liquid products.
25151069	6	43	theme	pine	1096:1099	arg1	wood					1101:1104	pine wood	1096:1104	pine wood	1096:1104	The formation of liquid compounds from pine wood was also observed to be influenced by the componential interactions.
25151069	3	44	theme	weight	746:751	arg1	compounds					760:768	smaller molecular weight liquid compounds	728:768	smaller molecular weight liquid compounds	728:768	Lignin accelerated the release of CO2 and CO from cellulose and intensified the decomposition of cellulose to smaller molecular weight liquid compounds.
25151069	4	45	theme	enhanced	885:892	arg1	yield					894:898	the enhanced yield	881:898	the enhanced yield of char	881:906	Pine wood exhibited the componential interactions, resulting in the broadened temperature range of mass loss, the enhanced yield of char, and the increased heterogeneity of char.
25151069	3	46	theme	CO2	652:654	arg1	release					641:647	the release	637:647	the release of CO2 and CO from cellulose	637:676	Lignin accelerated the release of CO2 and CO from cellulose and intensified the decomposition of cellulose to smaller molecular weight liquid compounds.
25151069	4	47	theme	Pine	771:774	arg1	wood					776:779	Pine wood	771:779	Pine wood	771:779	Pine wood exhibited the componential interactions, resulting in the broadened temperature range of mass loss, the enhanced yield of char, and the increased heterogeneity of char.
25151069	1	48	theme	release	473:479	arg1	rate					481:484	gas release rate	469:484	gas release rate	469:484	The pyrolysis characteristics of xylan, cellulose, ADF (a mixture of cellulose and lignin extracted from pine wood) and pine wood were investigated in a fixed-bed reactor by determining the distributions of three-phase products, the elemental compositions of char products, the conversions of components and the profiles of gas release rate during pyrolysis as well as the compositions of liquid products.
25151069	1	49	theme	rate	481:484	arg1	compositions					388:399	the elemental compositions	374:399	the elemental compositions of char products	374:416	The pyrolysis characteristics of xylan, cellulose, ADF (a mixture of cellulose and lignin extracted from pine wood) and pine wood were investigated in a fixed-bed reactor by determining the distributions of three-phase products, the elemental compositions of char products, the conversions of components and the profiles of gas release rate during pyrolysis as well as the compositions of liquid products.
25151069	1	49	theme	rate	481:484	arg1	profiles					457:464	the profiles	453:464	the profiles of gas release rate	453:484	The pyrolysis characteristics of xylan, cellulose, ADF (a mixture of cellulose and lignin extracted from pine wood) and pine wood were investigated in a fixed-bed reactor by determining the distributions of three-phase products, the elemental compositions of char products, the conversions of components and the profiles of gas release rate during pyrolysis as well as the compositions of liquid products.
25151069	1	49	theme	rate	481:484	arg1	conversions					423:433	the conversions	419:433	the conversions of components	419:447	The pyrolysis characteristics of xylan, cellulose, ADF (a mixture of cellulose and lignin extracted from pine wood) and pine wood were investigated in a fixed-bed reactor by determining the distributions of three-phase products, the elemental compositions of char products, the conversions of components and the profiles of gas release rate during pyrolysis as well as the compositions of liquid products.
25151069	1	49	theme	rate	481:484	arg1	distributions					335:347	the distributions	331:347	the distributions of three-phase products	331:371	The pyrolysis characteristics of xylan, cellulose, ADF (a mixture of cellulose and lignin extracted from pine wood) and pine wood were investigated in a fixed-bed reactor by determining the distributions of three-phase products, the elemental compositions of char products, the conversions of components and the profiles of gas release rate during pyrolysis as well as the compositions of liquid products.
25151069	6	50	theme	compounds	1081:1089	arg1	formation					1061:1069	The formation	1057:1069	The formation of liquid compounds from pine wood	1057:1104	The formation of liquid compounds from pine wood was also observed to be influenced by the componential interactions.
25151069	4	51	theme	char	944:947	arg1	range					861:865	the broadened temperature range	835:865	the broadened temperature range of mass loss	835:878	Pine wood exhibited the componential interactions, resulting in the broadened temperature range of mass loss, the enhanced yield of char, and the increased heterogeneity of char.
25151069	4	51	theme	char	944:947	arg1	heterogeneity					927:939	the increased heterogeneity	913:939	the increased heterogeneity of char	913:947	Pine wood exhibited the componential interactions, resulting in the broadened temperature range of mass loss, the enhanced yield of char, and the increased heterogeneity of char.
25151069	4	51	theme	char	944:947	arg1	yield					894:898	the enhanced yield	881:898	the enhanced yield of char	881:906	Pine wood exhibited the componential interactions, resulting in the broadened temperature range of mass loss, the enhanced yield of char, and the increased heterogeneity of char.
25151069	1	52	theme	char	404:407	arg1	products					409:416	char products	404:416	char products	404:416	The pyrolysis characteristics of xylan, cellulose, ADF (a mixture of cellulose and lignin extracted from pine wood) and pine wood were investigated in a fixed-bed reactor by determining the distributions of three-phase products, the elemental compositions of char products, the conversions of components and the profiles of gas release rate during pyrolysis as well as the compositions of liquid products.
25151069	6	53	theme	liquid	1074:1079	arg1	compounds					1081:1089	liquid compounds	1074:1089	liquid compounds from pine wood	1074:1104	The formation of liquid compounds from pine wood was also observed to be influenced by the componential interactions.
25151069	1	54	theme	cellulose	214:222	arg1	mixture					203:209	a mixture	201:209	a mixture of cellulose and lignin extracted from pine wood	201:258	The pyrolysis characteristics of xylan, cellulose, ADF (a mixture of cellulose and lignin extracted from pine wood) and pine wood were investigated in a fixed-bed reactor by determining the distributions of three-phase products, the elemental compositions of char products, the conversions of components and the profiles of gas release rate during pyrolysis as well as the compositions of liquid products.
25151069	0	55	theme	char	60:63	arg1	behaviors					47:55	the formation behaviors	33:55	the formation behaviors of char	33:63	A comparative investigation into the formation behaviors of char, liquids and gases during pyrolysis of pinewood and lignocellulosic components.
25151069	1	56	dep	ADF	196:198	arg1	mixture					203:209	a mixture	201:209	a mixture of cellulose and lignin extracted from pine wood	201:258	The pyrolysis characteristics of xylan, cellulose, ADF (a mixture of cellulose and lignin extracted from pine wood) and pine wood were investigated in a fixed-bed reactor by determining the distributions of three-phase products, the elemental compositions of char products, the conversions of components and the profiles of gas release rate during pyrolysis as well as the compositions of liquid products.
25151069	6	57	from	wood	1101:1104	arg1	formation					1061:1069	The formation	1057:1069	The formation of liquid compounds from pine wood	1057:1104	The formation of liquid compounds from pine wood was also observed to be influenced by the componential interactions.
25151069	6	57	from	wood	1101:1104	arg1	compounds					1081:1089	liquid compounds	1074:1089	liquid compounds from pine wood	1074:1104	The formation of liquid compounds from pine wood was also observed to be influenced by the componential interactions.
27959901	6	0	theme	species	902:908	arg1	metabolites					876:886	surface associated metabolites	857:886	surface associated metabolites of both Fucus species	857:908	We analysed the chemical composition of surface associated metabolites of both Fucus species by means of gas chromatography-mass spectrometry (GC-MS) to describe temporal patterns in chemical surface composition.
27959901	4	1	from	study	518:522	arg1	composition					540:550	the seasonal composition	527:550	the seasonal composition of surface associated metabolites, responsible for much of the fouling control,	527:630	To date, a study on the seasonal composition of surface associated metabolites, responsible for much of the fouling control, has not been done.
27959901	10	2	theme	acids	1607:1611	arg1	up-regulation					1504:1516	A strong summerly up-regulation	1486:1516	A strong summerly up-regulation of eighteen saccharides and two hydroxy acids in F. vesiculosus as well as of four fatty acids and two saccharides in F. serratus	1486:1646	A strong summerly up-regulation of eighteen saccharides and two hydroxy acids in F. vesiculosus as well as of four fatty acids and two saccharides in F. serratus was observed.
27959901	7	3	theme	sampled	1116:1122	arg1	habitat					1124:1130	the sampled habitat	1112:1130	the sampled habitat	1112:1130	Additionally, we correlated abiotic and biotic parameters recorded monthly within the sampled habitat with the variation in the chemical surface landscape of Fucus.
27959901	9	4	theme	Fucus	1471:1475	arg1	species					1477:1483	both Fucus species	1466:1483	both Fucus species	1466:1483	Light and temperature explained most of the seasonal variability in surface metabolite composition of both Fucus species.
27959901	6	5	theme	metabolites	876:886	arg1	composition					842:852	the chemical composition	829:852	the chemical composition of surface associated metabolites of both Fucus species	829:908	We analysed the chemical composition of surface associated metabolites of both Fucus species by means of gas chromatography-mass spectrometry (GC-MS) to describe temporal patterns in chemical surface composition.
27959901	11	6	theme	up-regulated	1683:1694	arg1	molecules					1696:1704	these up-regulated molecules	1677:1704	these up-regulated molecules	1677:1704	We discuss how these up-regulated molecules may have a complex effect on associated microfoulers, both promoting or decreasing fouling depending on metabolite and bacterial identity.
27959901	6	7	theme	surface	857:863	arg1	metabolites					876:886	surface associated metabolites	857:886	surface associated metabolites of both Fucus species	857:908	We analysed the chemical composition of surface associated metabolites of both Fucus species by means of gas chromatography-mass spectrometry (GC-MS) to describe temporal patterns in chemical surface composition.
27959901	10	8	from	F.	1636:1637	arg1	up-regulation					1504:1516	A strong summerly up-regulation	1486:1516	A strong summerly up-regulation of eighteen saccharides and two hydroxy acids in F. vesiculosus as well as of four fatty acids and two saccharides in F. serratus	1486:1646	A strong summerly up-regulation of eighteen saccharides and two hydroxy acids in F. vesiculosus as well as of four fatty acids and two saccharides in F. serratus was observed.
27959901	8	9	theme	species	1266:1272	arg1	composition					1240:1250	the chemical surface composition	1219:1250	the chemical surface composition of both Fucus species	1219:1272	Our study revealed that the chemical surface composition of both Fucus species exhibits substantial seasonal differences between spring/summer and autumn/winter months.
27959901	11	10	theme	bacterial	1825:1833	arg1	identity					1835:1842	metabolite and bacterial identity	1810:1842	metabolite and bacterial identity	1810:1842	We discuss how these up-regulated molecules may have a complex effect on associated microfoulers, both promoting or decreasing fouling depending on metabolite and bacterial identity.
27959901	7	11	theme	biotic	1070:1075	arg1	parameters					1077:1086	abiotic and biotic parameters	1058:1086	abiotic and biotic parameters recorded monthly	1058:1103	Additionally, we correlated abiotic and biotic parameters recorded monthly within the sampled habitat with the variation in the chemical surface landscape of Fucus.
27959901	5	12	dep	intervals	748:756	arg1	six					759:761	six	759:761	six	759:761	We sampled individuals of the two co-occurring species F. vesiculosus and F. serratus at monthly intervals (six per species and month) during a one-year field study.
27959901	8	13	theme	substantial	1283:1293	arg1	differences					1304:1314	substantial seasonal differences	1283:1314	substantial seasonal differences between spring/summer and autumn/winter months	1283:1361	Our study revealed that the chemical surface composition of both Fucus species exhibits substantial seasonal differences between spring/summer and autumn/winter months.
27959901	7	14	theme	abiotic	1058:1064	arg1	parameters					1077:1086	abiotic and biotic parameters	1058:1086	abiotic and biotic parameters recorded monthly	1058:1103	Additionally, we correlated abiotic and biotic parameters recorded monthly within the sampled habitat with the variation in the chemical surface landscape of Fucus.
27959901	5	15	theme	one-year	795:802	arg1	study					810:814	a one-year field study	793:814	a one-year field study	793:814	We sampled individuals of the two co-occurring species F. vesiculosus and F. serratus at monthly intervals (six per species and month) during a one-year field study.
27959901	1	16	theme	thallus	210:216	arg1	surface					218:224	their outer thallus surface	198:224	their outer thallus surface	198:224	Perennial macroalgae within the genus Fucus are known to exude metabolites through their outer thallus surface.
27959901	8	17	theme	chemical	1223:1230	arg1	composition					1240:1250	the chemical surface composition	1219:1250	the chemical surface composition of both Fucus species	1219:1272	Our study revealed that the chemical surface composition of both Fucus species exhibits substantial seasonal differences between spring/summer and autumn/winter months.
27959901	6	18	theme	gas	922:924	arg1	GC-MS					960:964	GC-MS	960:964	GC-MS	960:964	We analysed the chemical composition of surface associated metabolites of both Fucus species by means of gas chromatography-mass spectrometry (GC-MS) to describe temporal patterns in chemical surface composition.
27959901	6	18	theme	gas	922:924	arg1	spectrometry					946:957	gas chromatography-mass spectrometry	922:957	gas chromatography-mass spectrometry (GC-MS)	922:965	We analysed the chemical composition of surface associated metabolites of both Fucus species by means of gas chromatography-mass spectrometry (GC-MS) to describe temporal patterns in chemical surface composition.
27959901	3	19	theme	Seasonal	294:301	arg1	fluctuations					303:314	Seasonal fluctuations	294:314	Seasonal fluctuations of natural fouling pressure and chemical fouling control strength against micro- and macrofoulers	294:412	Seasonal fluctuations of natural fouling pressure and chemical fouling control strength against micro- and macrofoulers have previously been observed in Fucus, suggesting that control strength varies with threat.
27959901	1	20	theme	Perennial	115:123	arg1	macroalgae					125:134	Perennial macroalgae	115:134	Perennial macroalgae within the genus Fucus	115:157	Perennial macroalgae within the genus Fucus are known to exude metabolites through their outer thallus surface.
27959901	5	21	dep	F.	725:726	arg1	serratus					728:735	F. serratus	725:735	F. serratus	725:735	We sampled individuals of the two co-occurring species F. vesiculosus and F. serratus at monthly intervals (six per species and month) during a one-year field study.
27959901	6	22	dep	spectrometry	946:957	arg1	means					913:917	means	913:917	means	913:917	We analysed the chemical composition of surface associated metabolites of both Fucus species by means of gas chromatography-mass spectrometry (GC-MS) to describe temporal patterns in chemical surface composition.
27959901	8	23	theme	spring/summer	1324:1336	arg1	months					1356:1361	spring/summer and autumn/winter months	1324:1361	spring/summer and autumn/winter months	1324:1361	Our study revealed that the chemical surface composition of both Fucus species exhibits substantial seasonal differences between spring/summer and autumn/winter months.
27959901	5	24	theme	co-occurring	685:696	arg1	F.					706:707	F.	706:707	F.	706:707	We sampled individuals of the two co-occurring species F. vesiculosus and F. serratus at monthly intervals (six per species and month) during a one-year field study.
27959901	5	24	theme	co-occurring	685:696	arg1	F.					725:726	F.	725:726	F.	725:726	We sampled individuals of the two co-occurring species F. vesiculosus and F. serratus at monthly intervals (six per species and month) during a one-year field study.
27959901	5	24	theme	co-occurring	685:696	arg1	species					698:704	the two co-occurring species	677:704	the two co-occurring species F. vesiculosus and F. serratus	677:735	We sampled individuals of the two co-occurring species F. vesiculosus and F. serratus at monthly intervals (six per species and month) during a one-year field study.
27959901	6	25	theme	surface	1009:1015	arg1	composition					1017:1027	chemical surface composition	1000:1027	chemical surface composition	1000:1027	We analysed the chemical composition of surface associated metabolites of both Fucus species by means of gas chromatography-mass spectrometry (GC-MS) to describe temporal patterns in chemical surface composition.
27959901	8	26	theme	autumn/winter	1342:1354	arg1	months					1356:1361	spring/summer and autumn/winter months	1324:1361	spring/summer and autumn/winter months	1324:1361	Our study revealed that the chemical surface composition of both Fucus species exhibits substantial seasonal differences between spring/summer and autumn/winter months.
27959901	0	27	theme	vesiculosus	63:73	arg1	Composition					42:52	Surface Metabolite Composition	23:52	Surface Metabolite Composition of Fucus vesiculosus and Fucus serratus from the Baltic Sea	23:112	Seasonal Variations in Surface Metabolite Composition of Fucus vesiculosus and Fucus serratus from the Baltic Sea.
27959901	7	28	theme	surface	1167:1173	arg1	landscape					1175:1183	the chemical surface landscape	1154:1183	the chemical surface landscape of Fucus	1154:1192	Additionally, we correlated abiotic and biotic parameters recorded monthly within the sampled habitat with the variation in the chemical surface landscape of Fucus.
27959901	3	29	theme	fouling	327:333	arg1	pressure					335:342	natural fouling pressure	319:342	natural fouling pressure	319:342	Seasonal fluctuations of natural fouling pressure and chemical fouling control strength against micro- and macrofoulers have previously been observed in Fucus, suggesting that control strength varies with threat.
27959901	7	30	from	variation	1141:1149	arg1	landscape					1175:1183	the chemical surface landscape	1154:1183	the chemical surface landscape of Fucus	1154:1192	Additionally, we correlated abiotic and biotic parameters recorded monthly within the sampled habitat with the variation in the chemical surface landscape of Fucus.
27959901	10	31	theme	strong	1488:1493	arg1	up-regulation					1504:1516	A strong summerly up-regulation	1486:1516	A strong summerly up-regulation of eighteen saccharides and two hydroxy acids in F. vesiculosus as well as of four fatty acids and two saccharides in F. serratus	1486:1646	A strong summerly up-regulation of eighteen saccharides and two hydroxy acids in F. vesiculosus as well as of four fatty acids and two saccharides in F. serratus was observed.
27959901	11	32	theme	metabolite	1810:1819	arg1	identity					1835:1842	metabolite and bacterial identity	1810:1842	metabolite and bacterial identity	1810:1842	We discuss how these up-regulated molecules may have a complex effect on associated microfoulers, both promoting or decreasing fouling depending on metabolite and bacterial identity.
27959901	10	33	dep	F.	1567:1568	arg1	vesiculosus					1570:1580	F. vesiculosus	1567:1580	F. vesiculosus	1567:1580	A strong summerly up-regulation of eighteen saccharides and two hydroxy acids in F. vesiculosus as well as of four fatty acids and two saccharides in F. serratus was observed.
27959901	6	34	theme	temporal	979:986	arg1	patterns					988:995	temporal patterns	979:995	temporal patterns in chemical surface composition	979:1027	We analysed the chemical composition of surface associated metabolites of both Fucus species by means of gas chromatography-mass spectrometry (GC-MS) to describe temporal patterns in chemical surface composition.
27959901	3	35	theme	strength	373:380	arg1	fluctuations					303:314	Seasonal fluctuations	294:314	Seasonal fluctuations of natural fouling pressure and chemical fouling control strength against micro- and macrofoulers	294:412	Seasonal fluctuations of natural fouling pressure and chemical fouling control strength against micro- and macrofoulers have previously been observed in Fucus, suggesting that control strength varies with threat.
27959901	3	36	theme	fouling	357:363	arg1	strength					373:380	chemical fouling control strength	348:380	chemical fouling control strength	348:380	Seasonal fluctuations of natural fouling pressure and chemical fouling control strength against micro- and macrofoulers have previously been observed in Fucus, suggesting that control strength varies with threat.
27959901	5	37	theme	monthly	740:746	arg1	intervals					748:756	monthly intervals	740:756	monthly intervals (six per species and month) during a one-year field study	740:814	We sampled individuals of the two co-occurring species F. vesiculosus and F. serratus at monthly intervals (six per species and month) during a one-year field study.
27959901	11	38	contain	have	1710:1713	arg1	molecules					1696:1704	these up-regulated molecules	1677:1704	these up-regulated molecules	1677:1704	We discuss how these up-regulated molecules may have a complex effect on associated microfoulers, both promoting or decreasing fouling depending on metabolite and bacterial identity.
27959901	11	38	contain	have	1710:1713	arg2	effect					1725:1730	a complex effect	1715:1730	a complex effect	1715:1730	We discuss how these up-regulated molecules may have a complex effect on associated microfoulers, both promoting or decreasing fouling depending on metabolite and bacterial identity.
27959901	6	39	from	patterns	988:995	arg1	composition					1017:1027	chemical surface composition	1000:1027	chemical surface composition	1000:1027	We analysed the chemical composition of surface associated metabolites of both Fucus species by means of gas chromatography-mass spectrometry (GC-MS) to describe temporal patterns in chemical surface composition.
27959901	0	40	theme	Seasonal	0:7	arg1	Variations					9:18	Seasonal Variations	0:18	Seasonal Variations in Surface Metabolite Composition of Fucus vesiculosus and Fucus serratus from the Baltic Sea.	0:113	Seasonal Variations in Surface Metabolite Composition of Fucus vesiculosus and Fucus serratus from the Baltic Sea.
27959901	4	41	theme	surface	555:561	arg1	metabolites					574:584	surface associated metabolites	555:584	surface associated metabolites	555:584	To date, a study on the seasonal composition of surface associated metabolites, responsible for much of the fouling control, has not been done.
27959901	10	42	theme	hydroxy	1550:1556	arg1	acids					1558:1562	two hydroxy acids	1546:1562	two hydroxy acids	1546:1562	A strong summerly up-regulation of eighteen saccharides and two hydroxy acids in F. vesiculosus as well as of four fatty acids and two saccharides in F. serratus was observed.
27959901	5	43	theme	field	804:808	arg1	study					810:814	a one-year field study	793:814	a one-year field study	793:814	We sampled individuals of the two co-occurring species F. vesiculosus and F. serratus at monthly intervals (six per species and month) during a one-year field study.
27959901	4	44	theme	metabolites	574:584	arg1	composition					540:550	the seasonal composition	527:550	the seasonal composition of surface associated metabolites, responsible for much of the fouling control,	527:630	To date, a study on the seasonal composition of surface associated metabolites, responsible for much of the fouling control, has not been done.
27959901	3	45	theme	control	470:476	arg1	strength					478:485	control strength	470:485	control strength	470:485	Seasonal fluctuations of natural fouling pressure and chemical fouling control strength against micro- and macrofoulers have previously been observed in Fucus, suggesting that control strength varies with threat.
27959901	0	46	theme	Metabolite	31:40	arg1	Composition					42:52	Surface Metabolite Composition	23:52	Surface Metabolite Composition of Fucus vesiculosus and Fucus serratus from the Baltic Sea	23:112	Seasonal Variations in Surface Metabolite Composition of Fucus vesiculosus and Fucus serratus from the Baltic Sea.
27959901	12	47	theme	compound	1909:1916	arg1	control					1918:1924	a compound control	1907:1924	a compound control of density and composition of the Fucus associated biofilm	1907:1983	These seasonal shifts in the surface metabolome seem to exert a compound control of density and composition of the Fucus associated biofilm.
27959901	6	48	theme	chemical	833:840	arg1	composition					842:852	the chemical composition	829:852	the chemical composition of surface associated metabolites of both Fucus species	829:908	We analysed the chemical composition of surface associated metabolites of both Fucus species by means of gas chromatography-mass spectrometry (GC-MS) to describe temporal patterns in chemical surface composition.
27959901	11	49	theme	complex	1717:1723	arg1	effect					1725:1730	a complex effect	1715:1730	a complex effect	1715:1730	We discuss how these up-regulated molecules may have a complex effect on associated microfoulers, both promoting or decreasing fouling depending on metabolite and bacterial identity.
27959901	12	50	theme	surface	1874:1880	arg1	metabolome					1882:1891	the surface metabolome	1870:1891	the surface metabolome	1870:1891	These seasonal shifts in the surface metabolome seem to exert a compound control of density and composition of the Fucus associated biofilm.
27959901	9	51	theme	surface	1432:1438	arg1	composition					1451:1461	surface metabolite composition	1432:1461	surface metabolite composition of both Fucus species	1432:1483	Light and temperature explained most of the seasonal variability in surface metabolite composition of both Fucus species.
27959901	10	52	from	saccharides	1621:1631	arg1	F.					1636:1637	F.	1636:1637	F.	1636:1637	A strong summerly up-regulation of eighteen saccharides and two hydroxy acids in F. vesiculosus as well as of four fatty acids and two saccharides in F. serratus was observed.
27959901	5	53	dep	species	698:704	arg1	F.					706:707	F.	706:707	F.	706:707	We sampled individuals of the two co-occurring species F. vesiculosus and F. serratus at monthly intervals (six per species and month) during a one-year field study.
27959901	5	53	dep	species	698:704	arg1	F.					725:726	F.	725:726	F.	725:726	We sampled individuals of the two co-occurring species F. vesiculosus and F. serratus at monthly intervals (six per species and month) during a one-year field study.
27959901	5	53	dep	species	698:704	arg1	species					698:704	the two co-occurring species	677:704	the two co-occurring species F. vesiculosus and F. serratus	677:735	We sampled individuals of the two co-occurring species F. vesiculosus and F. serratus at monthly intervals (six per species and month) during a one-year field study.
27959901	6	54	theme	Fucus	896:900	arg1	species					902:908	both Fucus species	891:908	both Fucus species	891:908	We analysed the chemical composition of surface associated metabolites of both Fucus species by means of gas chromatography-mass spectrometry (GC-MS) to describe temporal patterns in chemical surface composition.
27959901	4	55	theme	responsible	587:597	arg1	metabolites					574:584	surface associated metabolites	555:584	surface associated metabolites	555:584	To date, a study on the seasonal composition of surface associated metabolites, responsible for much of the fouling control, has not been done.
27959901	2	56	contain	have	253:256	arg1	Some					227:230	Some	227:230	Some	227:230	Some of these metabolites have pro- and/or antifouling properties.
27959901	2	56	contain	have	253:256	arg1	metabolites					241:251	these metabolites	235:251	these metabolites	235:251	Some of these metabolites have pro- and/or antifouling properties.
27959901	2	56	contain	have	253:256	arg2	properties					282:291	pro- and/or antifouling properties	258:291	pro- and/or antifouling properties	258:291	Some of these metabolites have pro- and/or antifouling properties.
27959901	10	57	theme	fatty	1601:1605	arg1	acids					1607:1611	four fatty acids	1596:1611	four fatty acids	1596:1611	A strong summerly up-regulation of eighteen saccharides and two hydroxy acids in F. vesiculosus as well as of four fatty acids and two saccharides in F. serratus was observed.
27959901	2	58	theme	antifouling	270:280	arg1	properties					282:291	pro- and/or antifouling properties	258:291	pro- and/or antifouling properties	258:291	Some of these metabolites have pro- and/or antifouling properties.
27959901	9	59	theme	species	1477:1483	arg1	composition					1451:1461	surface metabolite composition	1432:1461	surface metabolite composition of both Fucus species	1432:1483	Light and temperature explained most of the seasonal variability in surface metabolite composition of both Fucus species.
27959901	6	60	theme	associated	865:874	arg1	metabolites					876:886	surface associated metabolites	857:886	surface associated metabolites of both Fucus species	857:908	We analysed the chemical composition of surface associated metabolites of both Fucus species by means of gas chromatography-mass spectrometry (GC-MS) to describe temporal patterns in chemical surface composition.
27959901	8	61	theme	surface	1232:1238	arg1	composition					1240:1250	the chemical surface composition	1219:1250	the chemical surface composition of both Fucus species	1219:1272	Our study revealed that the chemical surface composition of both Fucus species exhibits substantial seasonal differences between spring/summer and autumn/winter months.
27959901	5	62	dep	F.	706:707	arg1	vesiculosus					709:719	F. vesiculosus	706:719	F. vesiculosus	706:719	We sampled individuals of the two co-occurring species F. vesiculosus and F. serratus at monthly intervals (six per species and month) during a one-year field study.
27959901	10	63	dep	F.	1636:1637	arg1	serratus					1639:1646	F. serratus	1636:1646	F. serratus	1636:1646	A strong summerly up-regulation of eighteen saccharides and two hydroxy acids in F. vesiculosus as well as of four fatty acids and two saccharides in F. serratus was observed.
27959901	10	64	theme	saccharides	1621:1631	arg1	up-regulation					1504:1516	A strong summerly up-regulation	1486:1516	A strong summerly up-regulation of eighteen saccharides and two hydroxy acids in F. vesiculosus as well as of four fatty acids and two saccharides in F. serratus	1486:1646	A strong summerly up-regulation of eighteen saccharides and two hydroxy acids in F. vesiculosus as well as of four fatty acids and two saccharides in F. serratus was observed.
27959901	5	65	theme	species	698:704	arg1	individuals					662:672	individuals	662:672	individuals of the two co-occurring species F. vesiculosus and F. serratus	662:735	We sampled individuals of the two co-occurring species F. vesiculosus and F. serratus at monthly intervals (six per species and month) during a one-year field study.
27959901	8	66	theme	Fucus	1260:1264	arg1	species					1266:1272	both Fucus species	1255:1272	both Fucus species	1255:1272	Our study revealed that the chemical surface composition of both Fucus species exhibits substantial seasonal differences between spring/summer and autumn/winter months.
27959901	12	67	dep	associated	1966:1975	arg1	Fucus					1960:1964	Fucus	1960:1964	Fucus	1960:1964	These seasonal shifts in the surface metabolome seem to exert a compound control of density and composition of the Fucus associated biofilm.
27959901	1	68	theme	outer	204:208	arg1	surface					218:224	their outer thallus surface	198:224	their outer thallus surface	198:224	Perennial macroalgae within the genus Fucus are known to exude metabolites through their outer thallus surface.
27959901	12	69	theme	biofilm	1977:1983	arg1	composition					1941:1951	composition	1941:1951	composition	1941:1951	These seasonal shifts in the surface metabolome seem to exert a compound control of density and composition of the Fucus associated biofilm.
27959901	12	69	theme	biofilm	1977:1983	arg1	density					1929:1935	density	1929:1935	density	1929:1935	These seasonal shifts in the surface metabolome seem to exert a compound control of density and composition of the Fucus associated biofilm.
27959901	6	70	theme	chromatography-mass	926:944	arg1	GC-MS					960:964	GC-MS	960:964	GC-MS	960:964	We analysed the chemical composition of surface associated metabolites of both Fucus species by means of gas chromatography-mass spectrometry (GC-MS) to describe temporal patterns in chemical surface composition.
27959901	6	70	theme	chromatography-mass	926:944	arg1	spectrometry					946:957	gas chromatography-mass spectrometry	922:957	gas chromatography-mass spectrometry (GC-MS)	922:965	We analysed the chemical composition of surface associated metabolites of both Fucus species by means of gas chromatography-mass spectrometry (GC-MS) to describe temporal patterns in chemical surface composition.
27959901	11	71	dep	have	1710:1713	arg1	decreasing					1778:1787	decreasing	1778:1787	decreasing fouling depending on metabolite and bacterial identity	1778:1842	We discuss how these up-regulated molecules may have a complex effect on associated microfoulers, both promoting or decreasing fouling depending on metabolite and bacterial identity.
27959901	11	71	dep	have	1710:1713	arg1	promoting					1765:1773	promoting	1765:1773	promoting	1765:1773	We discuss how these up-regulated molecules may have a complex effect on associated microfoulers, both promoting or decreasing fouling depending on metabolite and bacterial identity.
27959901	3	72	theme	natural	319:325	arg1	pressure					335:342	natural fouling pressure	319:342	natural fouling pressure	319:342	Seasonal fluctuations of natural fouling pressure and chemical fouling control strength against micro- and macrofoulers have previously been observed in Fucus, suggesting that control strength varies with threat.
27959901	0	73	from	Sea	110:112	arg1	Composition					42:52	Surface Metabolite Composition	23:52	Surface Metabolite Composition of Fucus vesiculosus and Fucus serratus from the Baltic Sea	23:112	Seasonal Variations in Surface Metabolite Composition of Fucus vesiculosus and Fucus serratus from the Baltic Sea.
27959901	0	73	from	Sea	110:112	arg1	Variations					9:18	Seasonal Variations	0:18	Seasonal Variations in Surface Metabolite Composition of Fucus vesiculosus and Fucus serratus from the Baltic Sea.	0:113	Seasonal Variations in Surface Metabolite Composition of Fucus vesiculosus and Fucus serratus from the Baltic Sea.
27959901	8	74	theme	seasonal	1295:1302	arg1	differences					1304:1314	substantial seasonal differences	1283:1314	substantial seasonal differences between spring/summer and autumn/winter months	1283:1361	Our study revealed that the chemical surface composition of both Fucus species exhibits substantial seasonal differences between spring/summer and autumn/winter months.
27959901	7	75	theme	chemical	1158:1165	arg1	landscape					1175:1183	the chemical surface landscape	1154:1183	the chemical surface landscape of Fucus	1154:1192	Additionally, we correlated abiotic and biotic parameters recorded monthly within the sampled habitat with the variation in the chemical surface landscape of Fucus.
27959901	2	76	theme	pro-	258:261	arg1	properties					282:291	pro- and/or antifouling properties	258:291	pro- and/or antifouling properties	258:291	Some of these metabolites have pro- and/or antifouling properties.
27959901	6	77	theme	chemical	1000:1007	arg1	composition					1017:1027	chemical surface composition	1000:1027	chemical surface composition	1000:1027	We analysed the chemical composition of surface associated metabolites of both Fucus species by means of gas chromatography-mass spectrometry (GC-MS) to describe temporal patterns in chemical surface composition.
27959901	3	78	theme	chemical	348:355	arg1	strength					373:380	chemical fouling control strength	348:380	chemical fouling control strength	348:380	Seasonal fluctuations of natural fouling pressure and chemical fouling control strength against micro- and macrofoulers have previously been observed in Fucus, suggesting that control strength varies with threat.
27959901	0	79	theme	serratus	85:92	arg1	Composition					42:52	Surface Metabolite Composition	23:52	Surface Metabolite Composition of Fucus vesiculosus and Fucus serratus from the Baltic Sea	23:112	Seasonal Variations in Surface Metabolite Composition of Fucus vesiculosus and Fucus serratus from the Baltic Sea.
27959901	3	80	theme	pressure	335:342	arg1	fluctuations					303:314	Seasonal fluctuations	294:314	Seasonal fluctuations of natural fouling pressure and chemical fouling control strength against micro- and macrofoulers	294:412	Seasonal fluctuations of natural fouling pressure and chemical fouling control strength against micro- and macrofoulers have previously been observed in Fucus, suggesting that control strength varies with threat.
27959901	0	81	from	Variations	9:18	arg1	Composition					42:52	Surface Metabolite Composition	23:52	Surface Metabolite Composition of Fucus vesiculosus and Fucus serratus from the Baltic Sea	23:112	Seasonal Variations in Surface Metabolite Composition of Fucus vesiculosus and Fucus serratus from the Baltic Sea.
27959901	0	81	from	Variations	9:18	arg1	Sea					110:112	the Baltic Sea	99:112	the Baltic Sea	99:112	Seasonal Variations in Surface Metabolite Composition of Fucus vesiculosus and Fucus serratus from the Baltic Sea.
27959901	12	82	theme	associated	1966:1975	arg1	biofilm					1977:1983	the Fucus associated biofilm	1956:1983	the Fucus associated biofilm	1956:1983	These seasonal shifts in the surface metabolome seem to exert a compound control of density and composition of the Fucus associated biofilm.
27959901	7	83	theme	Fucus	1188:1192	arg1	landscape					1175:1183	the chemical surface landscape	1154:1183	the chemical surface landscape of Fucus	1154:1192	Additionally, we correlated abiotic and biotic parameters recorded monthly within the sampled habitat with the variation in the chemical surface landscape of Fucus.
27959901	12	84	theme	density	1929:1935	arg1	control					1918:1924	a compound control	1907:1924	a compound control of density and composition of the Fucus associated biofilm	1907:1983	These seasonal shifts in the surface metabolome seem to exert a compound control of density and composition of the Fucus associated biofilm.
27959901	3	85	theme	control	365:371	arg1	strength					373:380	chemical fouling control strength	348:380	chemical fouling control strength	348:380	Seasonal fluctuations of natural fouling pressure and chemical fouling control strength against micro- and macrofoulers have previously been observed in Fucus, suggesting that control strength varies with threat.
27959901	12	86	theme	composition	1941:1951	arg1	control					1918:1924	a compound control	1907:1924	a compound control of density and composition of the Fucus associated biofilm	1907:1983	These seasonal shifts in the surface metabolome seem to exert a compound control of density and composition of the Fucus associated biofilm.
27959901	4	87	theme	associated	563:572	arg1	metabolites					574:584	surface associated metabolites	555:584	surface associated metabolites	555:584	To date, a study on the seasonal composition of surface associated metabolites, responsible for much of the fouling control, has not been done.
27959901	10	88	theme	summerly	1495:1502	arg1	up-regulation					1504:1516	A strong summerly up-regulation	1486:1516	A strong summerly up-regulation of eighteen saccharides and two hydroxy acids in F. vesiculosus as well as of four fatty acids and two saccharides in F. serratus	1486:1646	A strong summerly up-regulation of eighteen saccharides and two hydroxy acids in F. vesiculosus as well as of four fatty acids and two saccharides in F. serratus was observed.
27959901	0	89	theme	Surface	23:29	arg1	Composition					42:52	Surface Metabolite Composition	23:52	Surface Metabolite Composition of Fucus vesiculosus and Fucus serratus from the Baltic Sea	23:112	Seasonal Variations in Surface Metabolite Composition of Fucus vesiculosus and Fucus serratus from the Baltic Sea.
27959901	1	90	dep	genus	147:151	arg1	Fucus					153:157	the genus Fucus	143:157	the genus Fucus	143:157	Perennial macroalgae within the genus Fucus are known to exude metabolites through their outer thallus surface.
27959901	4	91	theme	seasonal	531:538	arg1	composition					540:550	the seasonal composition	527:550	the seasonal composition of surface associated metabolites, responsible for much of the fouling control,	527:630	To date, a study on the seasonal composition of surface associated metabolites, responsible for much of the fouling control, has not been done.
27959901	10	92	theme	saccharides	1530:1540	arg1	up-regulation					1504:1516	A strong summerly up-regulation	1486:1516	A strong summerly up-regulation of eighteen saccharides and two hydroxy acids in F. vesiculosus as well as of four fatty acids and two saccharides in F. serratus	1486:1646	A strong summerly up-regulation of eighteen saccharides and two hydroxy acids in F. vesiculosus as well as of four fatty acids and two saccharides in F. serratus was observed.
27959901	0	93	theme	Baltic	103:108	arg1	Sea					110:112	the Baltic Sea	99:112	the Baltic Sea	99:112	Seasonal Variations in Surface Metabolite Composition of Fucus vesiculosus and Fucus serratus from the Baltic Sea.
27959901	10	94	from	up-regulation	1504:1516	arg1	F.					1636:1637	F.	1636:1637	F.	1636:1637	A strong summerly up-regulation of eighteen saccharides and two hydroxy acids in F. vesiculosus as well as of four fatty acids and two saccharides in F. serratus was observed.
27959901	10	94	from	up-regulation	1504:1516	arg1	F.					1567:1568	F.	1567:1568	F.	1567:1568	A strong summerly up-regulation of eighteen saccharides and two hydroxy acids in F. vesiculosus as well as of four fatty acids and two saccharides in F. serratus was observed.
27959901	12	95	theme	seasonal	1851:1858	arg1	shifts					1860:1865	These seasonal shifts	1845:1865	These seasonal shifts in the surface metabolome	1845:1891	These seasonal shifts in the surface metabolome seem to exert a compound control of density and composition of the Fucus associated biofilm.
27959901	9	96	theme	seasonal	1408:1415	arg1	variability					1417:1427	the seasonal variability	1404:1427	the seasonal variability in surface metabolite composition of both Fucus species	1404:1483	Light and temperature explained most of the seasonal variability in surface metabolite composition of both Fucus species.
27959901	9	97	from	variability	1417:1427	arg1	composition					1451:1461	surface metabolite composition	1432:1461	surface metabolite composition of both Fucus species	1432:1483	Light and temperature explained most of the seasonal variability in surface metabolite composition of both Fucus species.
27959901	12	98	from	shifts	1860:1865	arg1	metabolome					1882:1891	the surface metabolome	1870:1891	the surface metabolome	1870:1891	These seasonal shifts in the surface metabolome seem to exert a compound control of density and composition of the Fucus associated biofilm.
27959901	4	99	theme	fouling	615:621	arg1	control					623:629	the fouling control	611:629	the fouling control	611:629	To date, a study on the seasonal composition of surface associated metabolites, responsible for much of the fouling control, has not been done.
27959901	10	100	theme	acids	1558:1562	arg1	up-regulation					1504:1516	A strong summerly up-regulation	1486:1516	A strong summerly up-regulation of eighteen saccharides and two hydroxy acids in F. vesiculosus as well as of four fatty acids and two saccharides in F. serratus	1486:1646	A strong summerly up-regulation of eighteen saccharides and two hydroxy acids in F. vesiculosus as well as of four fatty acids and two saccharides in F. serratus was observed.
27959901	11	101	theme	associated	1735:1744	arg1	microfoulers					1746:1757	associated microfoulers	1735:1757	associated microfoulers	1735:1757	We discuss how these up-regulated molecules may have a complex effect on associated microfoulers, both promoting or decreasing fouling depending on metabolite and bacterial identity.
27959901	10	102	from	acids	1607:1611	arg1	F.					1636:1637	F.	1636:1637	F.	1636:1637	A strong summerly up-regulation of eighteen saccharides and two hydroxy acids in F. vesiculosus as well as of four fatty acids and two saccharides in F. serratus was observed.
27959901	9	103	theme	metabolite	1440:1449	arg1	composition					1451:1461	surface metabolite composition	1432:1461	surface metabolite composition of both Fucus species	1432:1483	Light and temperature explained most of the seasonal variability in surface metabolite composition of both Fucus species.
26944455	3	0	theme	temperature	595:605	arg1	activity					526:533	photosynthetic activity	511:533	photosynthetic activity of the strain	511:547	In a second time, photosynthetic activity of the strain was monitored as a function of irradiance and temperature, allowing improvement of kinetic parameters of growth and EPS production.
26944455	3	0	theme	temperature	595:605	arg1	function					568:575	a function	566:575	a function of irradiance and temperature	566:605	In a second time, photosynthetic activity of the strain was monitored as a function of irradiance and temperature, allowing improvement of kinetic parameters of growth and EPS production.
26944455	4	1	theme	batch	904:908	arg1	culture					910:916	batch culture	904:916	batch culture	904:916	A semi-continuous culture, carried out with the Pm medium, an optimal irradiance and temperature of respectively 360μmolphotonsm(-2)s(-1) and 28°C led to an EPS process productivity of 0.031gh(-1) instead of 0.020gh(-1) in batch culture.
26944455	1	2	theme	N0.0205S0.0597P0.005	307:326	arg1	composition					292:302	an elemental composition	279:302	an elemental composition of N0.0205S0.0597P0.005	279:326	With the aim to optimize the production of exopolysaccharide (EPS) by Porphyridium marinum, cultures in photobioreactors were conducted on a modified Provasoli medium (P) and compared to a new medium (Pm) with an elemental composition of N0.0205S0.0597P0.005.
26944455	4	3	theme	360μmolphotonsm	794:808	arg1	-1					815:816	-1	815:816	-1	815:816	A semi-continuous culture, carried out with the Pm medium, an optimal irradiance and temperature of respectively 360μmolphotonsm(-2)s(-1) and 28°C led to an EPS process productivity of 0.031gh(-1) instead of 0.020gh(-1) in batch culture.
26944455	4	3	theme	360μmolphotonsm	794:808	arg1	s					813:813	respectively 360μmolphotonsm(-2)s	781:813	respectively 360μmolphotonsm(-2)s(-1)	781:817	A semi-continuous culture, carried out with the Pm medium, an optimal irradiance and temperature of respectively 360μmolphotonsm(-2)s(-1) and 28°C led to an EPS process productivity of 0.031gh(-1) instead of 0.020gh(-1) in batch culture.
26944455	4	4	theme	28°C	823:826	arg1	irradiance					751:760	an optimal irradiance	740:760	an optimal irradiance	740:760	A semi-continuous culture, carried out with the Pm medium, an optimal irradiance and temperature of respectively 360μmolphotonsm(-2)s(-1) and 28°C led to an EPS process productivity of 0.031gh(-1) instead of 0.020gh(-1) in batch culture.
26944455	4	4	theme	28°C	823:826	arg1	temperature					766:776	temperature	766:776	temperature	766:776	A semi-continuous culture, carried out with the Pm medium, an optimal irradiance and temperature of respectively 360μmolphotonsm(-2)s(-1) and 28°C led to an EPS process productivity of 0.031gh(-1) instead of 0.020gh(-1) in batch culture.
26944455	4	4	theme	28°C	823:826	arg1	culture					699:705	A semi-continuous culture	681:705	A semi-continuous culture	681:705	A semi-continuous culture, carried out with the Pm medium, an optimal irradiance and temperature of respectively 360μmolphotonsm(-2)s(-1) and 28°C led to an EPS process productivity of 0.031gh(-1) instead of 0.020gh(-1) in batch culture.
26944455	3	5	theme	parameters	640:649	arg1	improvement					617:627	improvement	617:627	improvement of kinetic parameters of growth and EPS production	617:678	In a second time, photosynthetic activity of the strain was monitored as a function of irradiance and temperature, allowing improvement of kinetic parameters of growth and EPS production.
26944455	2	6	dep	2.5gL	404:408	arg1	up					398:399	up	398:399	up	398:399	Cultivation on this medium allowed the increase of EPS concentration up to 2.5gL(-1), without modification of the EPS productivity (0.096gL(-1)) and EPS structure.
26944455	2	7	from	Cultivation	329:339	arg1	medium					349:354	this medium	344:354	this medium	344:354	Cultivation on this medium allowed the increase of EPS concentration up to 2.5gL(-1), without modification of the EPS productivity (0.096gL(-1)) and EPS structure.
26944455	4	8	theme	semi-continuous	683:697	arg1	culture					699:705	A semi-continuous culture	681:705	A semi-continuous culture	681:705	A semi-continuous culture, carried out with the Pm medium, an optimal irradiance and temperature of respectively 360μmolphotonsm(-2)s(-1) and 28°C led to an EPS process productivity of 0.031gh(-1) instead of 0.020gh(-1) in batch culture.
26944455	3	9	theme	kinetic	632:638	arg1	parameters					640:649	kinetic parameters	632:649	kinetic parameters of growth and EPS production	632:678	In a second time, photosynthetic activity of the strain was monitored as a function of irradiance and temperature, allowing improvement of kinetic parameters of growth and EPS production.
26944455	4	10	theme	process	842:848	arg1	productivity					850:861	an EPS process productivity	835:861	an EPS process productivity of 0.031gh(-1) instead of 0.020gh(-1) in batch culture	835:916	A semi-continuous culture, carried out with the Pm medium, an optimal irradiance and temperature of respectively 360μmolphotonsm(-2)s(-1) and 28°C led to an EPS process productivity of 0.031gh(-1) instead of 0.020gh(-1) in batch culture.
26944455	3	11	theme	EPS	665:667	arg1	production					669:678	EPS production	665:678	EPS production	665:678	In a second time, photosynthetic activity of the strain was monitored as a function of irradiance and temperature, allowing improvement of kinetic parameters of growth and EPS production.
26944455	4	12	theme	optimal	743:749	arg1	irradiance					751:760	an optimal irradiance	740:760	an optimal irradiance	740:760	A semi-continuous culture, carried out with the Pm medium, an optimal irradiance and temperature of respectively 360μmolphotonsm(-2)s(-1) and 28°C led to an EPS process productivity of 0.031gh(-1) instead of 0.020gh(-1) in batch culture.
26944455	0	13	theme	production	33:42	arg1	Improvement					0:10	Improvement	0:10	Improvement of exopolysaccharide production by Porphyridium marinum.	0:67	Improvement of exopolysaccharide production by Porphyridium marinum.
26944455	1	14	theme	new	258:260	arg1	medium					262:267	a new medium	256:267	a new medium (Pm)	256:272	With the aim to optimize the production of exopolysaccharide (EPS) by Porphyridium marinum, cultures in photobioreactors were conducted on a modified Provasoli medium (P) and compared to a new medium (Pm) with an elemental composition of N0.0205S0.0597P0.005.
26944455	1	14	theme	new	258:260	arg1	Pm					270:271	Pm	270:271	Pm	270:271	With the aim to optimize the production of exopolysaccharide (EPS) by Porphyridium marinum, cultures in photobioreactors were conducted on a modified Provasoli medium (P) and compared to a new medium (Pm) with an elemental composition of N0.0205S0.0597P0.005.
26944455	2	15	theme	EPS	478:480	arg1	structure					482:490	EPS structure	478:490	EPS structure	478:490	Cultivation on this medium allowed the increase of EPS concentration up to 2.5gL(-1), without modification of the EPS productivity (0.096gL(-1)) and EPS structure.
26944455	0	16	theme	exopolysaccharide	15:31	arg1	production					33:42	exopolysaccharide production	15:42	exopolysaccharide production	15:42	Improvement of exopolysaccharide production by Porphyridium marinum.
26944455	3	17	theme	growth	654:659	arg1	parameters					640:649	kinetic parameters	632:649	kinetic parameters of growth and EPS production	632:678	In a second time, photosynthetic activity of the strain was monitored as a function of irradiance and temperature, allowing improvement of kinetic parameters of growth and EPS production.
26944455	2	18	theme	concentration	384:396	arg1	increase					368:375	the increase	364:375	the increase of EPS concentration up to 2.5gL(-1)	364:412	Cultivation on this medium allowed the increase of EPS concentration up to 2.5gL(-1), without modification of the EPS productivity (0.096gL(-1)) and EPS structure.
26944455	0	19	theme	Porphyridium	47:58	arg1	marinum					60:66	Porphyridium marinum	47:66	Porphyridium marinum	47:66	Improvement of exopolysaccharide production by Porphyridium marinum.
26944455	4	20	theme	Pm	729:730	arg1	medium					732:737	the Pm medium	725:737	the Pm medium	725:737	A semi-continuous culture, carried out with the Pm medium, an optimal irradiance and temperature of respectively 360μmolphotonsm(-2)s(-1) and 28°C led to an EPS process productivity of 0.031gh(-1) instead of 0.020gh(-1) in batch culture.
26944455	2	21	theme	EPS	380:382	arg1	concentration					384:396	EPS concentration	380:396	EPS concentration	380:396	Cultivation on this medium allowed the increase of EPS concentration up to 2.5gL(-1), without modification of the EPS productivity (0.096gL(-1)) and EPS structure.
26944455	4	22	theme	0.031gh	866:872	arg1	productivity					850:861	an EPS process productivity	835:861	an EPS process productivity of 0.031gh(-1) instead of 0.020gh(-1) in batch culture	835:916	A semi-continuous culture, carried out with the Pm medium, an optimal irradiance and temperature of respectively 360μmolphotonsm(-2)s(-1) and 28°C led to an EPS process productivity of 0.031gh(-1) instead of 0.020gh(-1) in batch culture.
26944455	3	23	theme	photosynthetic	511:524	arg1	function					568:575	a function	566:575	a function of irradiance and temperature	566:605	In a second time, photosynthetic activity of the strain was monitored as a function of irradiance and temperature, allowing improvement of kinetic parameters of growth and EPS production.
26944455	3	23	theme	photosynthetic	511:524	arg1	activity					526:533	photosynthetic activity	511:533	photosynthetic activity of the strain	511:547	In a second time, photosynthetic activity of the strain was monitored as a function of irradiance and temperature, allowing improvement of kinetic parameters of growth and EPS production.
26944455	1	24	theme	modified	210:217	arg1	P					237:237	P	237:237	P	237:237	With the aim to optimize the production of exopolysaccharide (EPS) by Porphyridium marinum, cultures in photobioreactors were conducted on a modified Provasoli medium (P) and compared to a new medium (Pm) with an elemental composition of N0.0205S0.0597P0.005.
26944455	1	24	theme	modified	210:217	arg1	medium					229:234	a modified Provasoli medium	208:234	a modified Provasoli medium (P)	208:238	With the aim to optimize the production of exopolysaccharide (EPS) by Porphyridium marinum, cultures in photobioreactors were conducted on a modified Provasoli medium (P) and compared to a new medium (Pm) with an elemental composition of N0.0205S0.0597P0.005.
26944455	3	25	theme	production	669:678	arg1	parameters					640:649	kinetic parameters	632:649	kinetic parameters of growth and EPS production	632:678	In a second time, photosynthetic activity of the strain was monitored as a function of irradiance and temperature, allowing improvement of kinetic parameters of growth and EPS production.
26944455	3	26	theme	second	498:503	arg1	time					505:508	a second time	496:508	a second time	496:508	In a second time, photosynthetic activity of the strain was monitored as a function of irradiance and temperature, allowing improvement of kinetic parameters of growth and EPS production.
26944455	1	27	theme	exopolysaccharide	112:128	arg1	production					98:107	the production	94:107	the production of exopolysaccharide (EPS) by Porphyridium marinum	94:158	With the aim to optimize the production of exopolysaccharide (EPS) by Porphyridium marinum, cultures in photobioreactors were conducted on a modified Provasoli medium (P) and compared to a new medium (Pm) with an elemental composition of N0.0205S0.0597P0.005.
26944455	3	28	theme	irradiance	580:589	arg1	activity					526:533	photosynthetic activity	511:533	photosynthetic activity of the strain	511:547	In a second time, photosynthetic activity of the strain was monitored as a function of irradiance and temperature, allowing improvement of kinetic parameters of growth and EPS production.
26944455	3	28	theme	irradiance	580:589	arg1	function					568:575	a function	566:575	a function of irradiance and temperature	566:605	In a second time, photosynthetic activity of the strain was monitored as a function of irradiance and temperature, allowing improvement of kinetic parameters of growth and EPS production.
26944455	4	29	theme	EPS	838:840	arg1	productivity					850:861	an EPS process productivity	835:861	an EPS process productivity of 0.031gh(-1) instead of 0.020gh(-1) in batch culture	835:916	A semi-continuous culture, carried out with the Pm medium, an optimal irradiance and temperature of respectively 360μmolphotonsm(-2)s(-1) and 28°C led to an EPS process productivity of 0.031gh(-1) instead of 0.020gh(-1) in batch culture.
26944455	4	30	theme	s	813:813	arg1	irradiance					751:760	an optimal irradiance	740:760	an optimal irradiance	740:760	A semi-continuous culture, carried out with the Pm medium, an optimal irradiance and temperature of respectively 360μmolphotonsm(-2)s(-1) and 28°C led to an EPS process productivity of 0.031gh(-1) instead of 0.020gh(-1) in batch culture.
26944455	4	30	theme	s	813:813	arg1	temperature					766:776	temperature	766:776	temperature	766:776	A semi-continuous culture, carried out with the Pm medium, an optimal irradiance and temperature of respectively 360μmolphotonsm(-2)s(-1) and 28°C led to an EPS process productivity of 0.031gh(-1) instead of 0.020gh(-1) in batch culture.
26944455	4	30	theme	s	813:813	arg1	culture					699:705	A semi-continuous culture	681:705	A semi-continuous culture	681:705	A semi-continuous culture, carried out with the Pm medium, an optimal irradiance and temperature of respectively 360μmolphotonsm(-2)s(-1) and 28°C led to an EPS process productivity of 0.031gh(-1) instead of 0.020gh(-1) in batch culture.
26944455	1	31	theme	Provasoli	219:227	arg1	P					237:237	P	237:237	P	237:237	With the aim to optimize the production of exopolysaccharide (EPS) by Porphyridium marinum, cultures in photobioreactors were conducted on a modified Provasoli medium (P) and compared to a new medium (Pm) with an elemental composition of N0.0205S0.0597P0.005.
26944455	1	31	theme	Provasoli	219:227	arg1	medium					229:234	a modified Provasoli medium	208:234	a modified Provasoli medium (P)	208:238	With the aim to optimize the production of exopolysaccharide (EPS) by Porphyridium marinum, cultures in photobioreactors were conducted on a modified Provasoli medium (P) and compared to a new medium (Pm) with an elemental composition of N0.0205S0.0597P0.005.
26944455	2	32	theme	structure	482:490	arg1	modification					423:434	modification	423:434	modification of the EPS productivity (0.096gL(-1)) and EPS structure	423:490	Cultivation on this medium allowed the increase of EPS concentration up to 2.5gL(-1), without modification of the EPS productivity (0.096gL(-1)) and EPS structure.
26944455	3	33	theme	strain	542:547	arg1	function					568:575	a function	566:575	a function of irradiance and temperature	566:605	In a second time, photosynthetic activity of the strain was monitored as a function of irradiance and temperature, allowing improvement of kinetic parameters of growth and EPS production.
26944455	3	33	theme	strain	542:547	arg1	activity					526:533	photosynthetic activity	511:533	photosynthetic activity of the strain	511:547	In a second time, photosynthetic activity of the strain was monitored as a function of irradiance and temperature, allowing improvement of kinetic parameters of growth and EPS production.
26944455	4	34	from	productivity	850:861	arg1	culture					910:916	batch culture	904:916	batch culture	904:916	A semi-continuous culture, carried out with the Pm medium, an optimal irradiance and temperature of respectively 360μmolphotonsm(-2)s(-1) and 28°C led to an EPS process productivity of 0.031gh(-1) instead of 0.020gh(-1) in batch culture.
26944455	2	35	theme	productivity	447:458	arg1	modification					423:434	modification	423:434	modification of the EPS productivity (0.096gL(-1)) and EPS structure	423:490	Cultivation on this medium allowed the increase of EPS concentration up to 2.5gL(-1), without modification of the EPS productivity (0.096gL(-1)) and EPS structure.
26944455	2	36	theme	EPS	443:445	arg1	0.096gL					461:467	0.096gL(-1)	461:471	0.096gL(-1)	461:471	Cultivation on this medium allowed the increase of EPS concentration up to 2.5gL(-1), without modification of the EPS productivity (0.096gL(-1)) and EPS structure.
26944455	2	36	theme	EPS	443:445	arg1	productivity					447:458	the EPS productivity	439:458	the EPS productivity (0.096gL(-1))	439:472	Cultivation on this medium allowed the increase of EPS concentration up to 2.5gL(-1), without modification of the EPS productivity (0.096gL(-1)) and EPS structure.
26944455	1	37	theme	elemental	282:290	arg1	composition					292:302	an elemental composition	279:302	an elemental composition of N0.0205S0.0597P0.005	279:326	With the aim to optimize the production of exopolysaccharide (EPS) by Porphyridium marinum, cultures in photobioreactors were conducted on a modified Provasoli medium (P) and compared to a new medium (Pm) with an elemental composition of N0.0205S0.0597P0.005.
26944455	4	38	theme	0.020gh	889:895	arg1	productivity					850:861	an EPS process productivity	835:861	an EPS process productivity of 0.031gh(-1) instead of 0.020gh(-1) in batch culture	835:916	A semi-continuous culture, carried out with the Pm medium, an optimal irradiance and temperature of respectively 360μmolphotonsm(-2)s(-1) and 28°C led to an EPS process productivity of 0.031gh(-1) instead of 0.020gh(-1) in batch culture.
26944455	1	39	from	cultures	161:168	arg1	photobioreactors					173:188	photobioreactors	173:188	photobioreactors	173:188	With the aim to optimize the production of exopolysaccharide (EPS) by Porphyridium marinum, cultures in photobioreactors were conducted on a modified Provasoli medium (P) and compared to a new medium (Pm) with an elemental composition of N0.0205S0.0597P0.005.
28224918	3	0	from	insights	495:502	arg1	phases					513:518	early phases	507:518	early phases of texture elaboration	507:541	Identification of key events and their kinetics with regard to tissue architecture and cell wall development can provide new insights on early phases of texture elaboration.
28224918	9	1	theme	young	1528:1532	arg1	fruit					1534:1538	young fruit	1528:1538	young fruit	1528:1538	In situ localization of cell wall polysaccharides in pericarp tissue revealed non-ramified RG-I rich pectin and XyG at cellular junctions and in the middle lamella of young fruit.
28224918	10	2	theme	middle	1680:1685	arg1	lamella					1687:1693	middle lamella	1680:1693	middle lamella	1680:1693	Blocks of non-methyl esterified homogalacturonan are detected as soon as 14 DPA in the mesocarp and remained restricted to cell corner and middle lamella whatever the stages.
28224918	9	3	theme	wall	1390:1393	arg1	polysaccharides					1395:1409	cell wall polysaccharides	1385:1409	cell wall polysaccharides	1385:1409	In situ localization of cell wall polysaccharides in pericarp tissue revealed non-ramified RG-I rich pectin and XyG at cellular junctions and in the middle lamella of young fruit.
28224918	4	4	dep	development	737:747	arg1	7					750:750	7	750:750	7	750:750	In this study, changes in pectin and hemicellulose chemical characteristics and location were investigated in the pericarp tissue of tomato (Solanum lycopersicon var Levovil) at four stages of development (7, 14 and 21day after anthesis (DPA) and mature green stages).
28224918	4	4	dep	development	737:747	arg1	21day					760:764	21day	760:764	21day	760:764	In this study, changes in pectin and hemicellulose chemical characteristics and location were investigated in the pericarp tissue of tomato (Solanum lycopersicon var Levovil) at four stages of development (7, 14 and 21day after anthesis (DPA) and mature green stages).
28224918	4	4	dep	development	737:747	arg1	14					753:754	14	753:754	14	753:754	In this study, changes in pectin and hemicellulose chemical characteristics and location were investigated in the pericarp tissue of tomato (Solanum lycopersicon var Levovil) at four stages of development (7, 14 and 21day after anthesis (DPA) and mature green stages).
28224918	4	5	theme	green	798:802	arg1	stages					804:809	mature green stages	791:809	mature green stages	791:809	In this study, changes in pectin and hemicellulose chemical characteristics and location were investigated in the pericarp tissue of tomato (Solanum lycopersicon var Levovil) at four stages of development (7, 14 and 21day after anthesis (DPA) and mature green stages).
28224918	4	6	theme	pericarp	658:665	arg1	tissue					667:672	the pericarp tissue	654:672	the pericarp tissue of tomato (Solanum lycopersicon var Levovil)	654:717	In this study, changes in pectin and hemicellulose chemical characteristics and location were investigated in the pericarp tissue of tomato (Solanum lycopersicon var Levovil) at four stages of development (7, 14 and 21day after anthesis (DPA) and mature green stages).
28224918	3	7	theme	texture	523:529	arg1	elaboration					531:541	texture elaboration	523:541	texture elaboration	523:541	Identification of key events and their kinetics with regard to tissue architecture and cell wall development can provide new insights on early phases of texture elaboration.
28224918	1	8	theme	genetic	161:167	arg1	controls					187:194	genetic and developmental controls	161:194	genetic and developmental controls	161:194	Tomato fruit texture depends on histology and cell wall architecture, both under genetic and developmental controls.
28224918	10	9	theme	esterified	1562:1571	arg1	homogalacturonan					1573:1588	non-methyl esterified homogalacturonan	1551:1588	non-methyl esterified homogalacturonan	1551:1588	Blocks of non-methyl esterified homogalacturonan are detected as soon as 14 DPA in the mesocarp and remained restricted to cell corner and middle lamella whatever the stages.
28224918	1	10	theme	developmental	173:185	arg1	controls					187:194	genetic and developmental controls	161:194	genetic and developmental controls	161:194	Tomato fruit texture depends on histology and cell wall architecture, both under genetic and developmental controls.
28224918	8	11	theme	Cell	1224:1227	arg1	remodelling					1249:1259	Cell wall polysaccharide remodelling	1224:1259	Cell wall polysaccharide remodelling	1224:1259	Cell wall polysaccharide remodelling also concerns xyloglucans and (galacto)glucomannans, the major hemicelluloses in tomato cell walls.
28224918	1	12	theme	fruit	87:91	arg1	texture					93:99	Tomato fruit texture	80:99	Tomato fruit texture	80:99	Tomato fruit texture depends on histology and cell wall architecture, both under genetic and developmental controls.
28224918	6	13	theme	rhamnogalacturonan	1043:1060	arg1	synthesis					1030:1038	a high synthesis	1023:1038	a high synthesis of rhamnogalacturonan I	1023:1062	At early stages, the relative high rhamnose content in cell walls indicates a high synthesis of rhamnogalacturonan I next to homogalacturonan.
28224918	9	14	theme	non-ramified	1439:1450	arg1	pectin					1462:1467	non-ramified RG-I rich pectin	1439:1467	non-ramified RG-I rich pectin	1439:1467	In situ localization of cell wall polysaccharides in pericarp tissue revealed non-ramified RG-I rich pectin and XyG at cellular junctions and in the middle lamella of young fruit.
28224918	8	15	theme	polysaccharide	1234:1247	arg1	remodelling					1249:1259	Cell wall polysaccharide remodelling	1224:1259	Cell wall polysaccharide remodelling	1224:1259	Cell wall polysaccharide remodelling also concerns xyloglucans and (galacto)glucomannans, the major hemicelluloses in tomato cell walls.
28224918	11	16	theme	RGI	1778:1780	arg1	role					1763:1766	the role	1759:1766	the role of pectin RGI and XyG in cell adhesion	1759:1805	These results point to new questions about the role of pectin RGI and XyG in cell adhesion and its maintenance during cell expansion.
28224918	11	16	theme	RGI	1778:1780	arg1	maintenance					1815:1825	its maintenance	1811:1825	its maintenance during cell expansion	1811:1847	These results point to new questions about the role of pectin RGI and XyG in cell adhesion and its maintenance during cell expansion.
28224918	2	17	theme	wall	222:225	arg1	modifications					227:239	ripening related cell wall modifications	200:239	ripening related cell wall modifications	200:239	If ripening related cell wall modifications have been well documented with regard to softening, little is known about cell wall construction during early fruit development.
28224918	9	18	theme	rich	1457:1460	arg1	pectin					1462:1467	non-ramified RG-I rich pectin	1439:1467	non-ramified RG-I rich pectin	1439:1467	In situ localization of cell wall polysaccharides in pericarp tissue revealed non-ramified RG-I rich pectin and XyG at cellular junctions and in the middle lamella of young fruit.
28224918	2	19	theme	related	209:215	arg1	modifications					227:239	ripening related cell wall modifications	200:239	ripening related cell wall modifications	200:239	If ripening related cell wall modifications have been well documented with regard to softening, little is known about cell wall construction during early fruit development.
28224918	8	20	theme	cell	1349:1352	arg1	walls					1354:1358	tomato cell walls	1342:1358	tomato cell walls	1342:1358	Cell wall polysaccharide remodelling also concerns xyloglucans and (galacto)glucomannans, the major hemicelluloses in tomato cell walls.
28224918	1	21	theme	cell	126:129	arg1	architecture					136:147	cell wall architecture	126:147	cell wall architecture	126:147	Tomato fruit texture depends on histology and cell wall architecture, both under genetic and developmental controls.
28224918	6	22	theme	rhamnose	982:989	arg1	content					991:997	the relative high rhamnose content	964:997	the relative high rhamnose content in cell walls	964:1011	At early stages, the relative high rhamnose content in cell walls indicates a high synthesis of rhamnogalacturonan I next to homogalacturonan.
28224918	10	23	dep	stages	1708:1713	arg1	whatever					1695:1702	whatever	1695:1702	whatever	1695:1702	Blocks of non-methyl esterified homogalacturonan are detected as soon as 14 DPA in the mesocarp and remained restricted to cell corner and middle lamella whatever the stages.
28224918	5	24	theme	composition	835:845	arg1	Analysis					813:820	Analysis	813:820	Analysis of cell wall composition and polysaccharide structure	813:874	Analysis of cell wall composition and polysaccharide structure revealed that both are continuously modified during fruit development.
28224918	6	25	theme	relative	968:975	arg1	content					991:997	the relative high rhamnose content	964:997	the relative high rhamnose content in cell walls	964:1011	At early stages, the relative high rhamnose content in cell walls indicates a high synthesis of rhamnogalacturonan I next to homogalacturonan.
28224918	4	26	theme	lycopersicon	693:704	arg1	Levovil					710:716	Solanum lycopersicon var Levovil	685:716	Solanum lycopersicon var Levovil	685:716	In this study, changes in pectin and hemicellulose chemical characteristics and location were investigated in the pericarp tissue of tomato (Solanum lycopersicon var Levovil) at four stages of development (7, 14 and 21day after anthesis (DPA) and mature green stages).
28224918	4	26	theme	lycopersicon	693:704	arg1	tomato					677:682	tomato	677:682	tomato (Solanum lycopersicon var Levovil)	677:717	In this study, changes in pectin and hemicellulose chemical characteristics and location were investigated in the pericarp tissue of tomato (Solanum lycopersicon var Levovil) at four stages of development (7, 14 and 21day after anthesis (DPA) and mature green stages).
28224918	5	27	theme	polysaccharide	851:864	arg1	structure					866:874	polysaccharide structure	851:874	polysaccharide structure	851:874	Analysis of cell wall composition and polysaccharide structure revealed that both are continuously modified during fruit development.
28224918	6	28	from	content	991:997	arg1	walls					1007:1011	cell walls	1002:1011	cell walls	1002:1011	At early stages, the relative high rhamnose content in cell walls indicates a high synthesis of rhamnogalacturonan I next to homogalacturonan.
28224918	7	29	theme	expansion	1169:1177	arg1	phase					1179:1183	the cell expansion phase	1160:1183	the cell expansion phase	1160:1183	Fine tuning of rhamnogalacturonan I side chains appears to occur from the cell expansion phase until prior to the mature green stage.
28224918	9	30	theme	middle	1510:1515	arg1	lamella					1517:1523	the middle lamella	1506:1523	the middle lamella of young fruit	1506:1538	In situ localization of cell wall polysaccharides in pericarp tissue revealed non-ramified RG-I rich pectin and XyG at cellular junctions and in the middle lamella of young fruit.
28224918	6	31	theme	early	950:954	arg1	stages					956:961	early stages	950:961	early stages	950:961	At early stages, the relative high rhamnose content in cell walls indicates a high synthesis of rhamnogalacturonan I next to homogalacturonan.
28224918	8	32	theme	major	1318:1322	arg1	xyloglucans					1275:1285	xyloglucans	1275:1285	xyloglucans	1275:1285	Cell wall polysaccharide remodelling also concerns xyloglucans and (galacto)glucomannans, the major hemicelluloses in tomato cell walls.
28224918	8	32	theme	major	1318:1322	arg1	hemicelluloses					1324:1337	the major hemicelluloses	1314:1337	the major hemicelluloses in tomato cell walls	1314:1358	Cell wall polysaccharide remodelling also concerns xyloglucans and (galacto)glucomannans, the major hemicelluloses in tomato cell walls.
28224918	4	33	from	changes	559:565	arg1	location					624:631	location	624:631	location	624:631	In this study, changes in pectin and hemicellulose chemical characteristics and location were investigated in the pericarp tissue of tomato (Solanum lycopersicon var Levovil) at four stages of development (7, 14 and 21day after anthesis (DPA) and mature green stages).
28224918	4	33	from	changes	559:565	arg1	characteristics					604:618	pectin and hemicellulose chemical characteristics	570:618	characteristics	604:618	In this study, changes in pectin and hemicellulose chemical characteristics and location were investigated in the pericarp tissue of tomato (Solanum lycopersicon var Levovil) at four stages of development (7, 14 and 21day after anthesis (DPA) and mature green stages).
28224918	11	34	theme	cell	1834:1837	arg1	expansion					1839:1847	cell expansion	1834:1847	cell expansion	1834:1847	These results point to new questions about the role of pectin RGI and XyG in cell adhesion and its maintenance during cell expansion.
28224918	9	35	theme	fruit	1534:1538	arg1	lamella					1517:1523	the middle lamella	1506:1523	the middle lamella of young fruit	1506:1538	In situ localization of cell wall polysaccharides in pericarp tissue revealed non-ramified RG-I rich pectin and XyG at cellular junctions and in the middle lamella of young fruit.
28224918	4	36	theme	development	737:747	arg1	stages					727:732	four stages	722:732	four stages of development (7, 14 and 21day after anthesis (DPA) and mature green stages)	722:810	In this study, changes in pectin and hemicellulose chemical characteristics and location were investigated in the pericarp tissue of tomato (Solanum lycopersicon var Levovil) at four stages of development (7, 14 and 21day after anthesis (DPA) and mature green stages).
28224918	3	37	theme	cell	457:460	arg1	development					467:477	cell wall development	457:477	cell wall development	457:477	Identification of key events and their kinetics with regard to tissue architecture and cell wall development can provide new insights on early phases of texture elaboration.
28224918	0	38	theme	pectin	14:19	arg1	Remodeling					0:9	Remodeling	0:9	Remodeling of pectin	0:19	Remodeling of pectin and hemicelluloses in tomato pericarp during fruit growth.
28224918	5	39	theme	wall	830:833	arg1	composition					835:845	cell wall composition	825:845	cell wall composition	825:845	Analysis of cell wall composition and polysaccharide structure revealed that both are continuously modified during fruit development.
28224918	7	40	theme	side	1126:1129	arg1	chains					1131:1136	rhamnogalacturonan I side chains	1105:1136	rhamnogalacturonan I side chains	1105:1136	Fine tuning of rhamnogalacturonan I side chains appears to occur from the cell expansion phase until prior to the mature green stage.
28224918	3	41	theme	events	392:397	arg1	Identification					370:383	Identification	370:383	Identification of key events and their kinetics with regard to tissue architecture and cell wall development	370:477	Identification of key events and their kinetics with regard to tissue architecture and cell wall development can provide new insights on early phases of texture elaboration.
28224918	11	42	theme	cell	1793:1796	arg1	adhesion					1798:1805	cell adhesion	1793:1805	cell adhesion	1793:1805	These results point to new questions about the role of pectin RGI and XyG in cell adhesion and its maintenance during cell expansion.
28224918	0	43	theme	tomato	43:48	arg1	pericarp					50:57	tomato pericarp	43:57	tomato pericarp	43:57	Remodeling of pectin and hemicelluloses in tomato pericarp during fruit growth.
28224918	2	44	theme	cell	315:318	arg1	construction					325:336	cell wall construction	315:336	cell wall construction	315:336	If ripening related cell wall modifications have been well documented with regard to softening, little is known about cell wall construction during early fruit development.
28224918	4	45	theme	hemicellulose	581:593	arg1	characteristics					604:618	pectin and hemicellulose chemical characteristics	570:618	characteristics	604:618	In this study, changes in pectin and hemicellulose chemical characteristics and location were investigated in the pericarp tissue of tomato (Solanum lycopersicon var Levovil) at four stages of development (7, 14 and 21day after anthesis (DPA) and mature green stages).
28224918	10	46	theme	cell	1664:1667	arg1	corner					1669:1674	cell corner	1664:1674	cell corner	1664:1674	Blocks of non-methyl esterified homogalacturonan are detected as soon as 14 DPA in the mesocarp and remained restricted to cell corner and middle lamella whatever the stages.
28224918	3	47	theme	early	507:511	arg1	phases					513:518	early phases	507:518	early phases of texture elaboration	507:541	Identification of key events and their kinetics with regard to tissue architecture and cell wall development can provide new insights on early phases of texture elaboration.
28224918	9	48	theme	In	1361:1362	arg1	localization					1369:1380	In situ localization	1361:1380	In situ localization of cell wall polysaccharides in pericarp tissue	1361:1428	In situ localization of cell wall polysaccharides in pericarp tissue revealed non-ramified RG-I rich pectin and XyG at cellular junctions and in the middle lamella of young fruit.
28224918	7	49	theme	Fine	1090:1093	arg1	tuning					1095:1100	Fine tuning	1090:1100	Fine tuning of rhamnogalacturonan I side chains	1090:1136	Fine tuning of rhamnogalacturonan I side chains appears to occur from the cell expansion phase until prior to the mature green stage.
28224918	2	50	theme	early	345:349	arg1	development					357:367	early fruit development	345:367	early fruit development	345:367	If ripening related cell wall modifications have been well documented with regard to softening, little is known about cell wall construction during early fruit development.
28224918	7	51	theme	mature	1204:1209	arg1	stage					1217:1221	the mature green stage	1200:1221	the mature green stage	1200:1221	Fine tuning of rhamnogalacturonan I side chains appears to occur from the cell expansion phase until prior to the mature green stage.
28224918	3	52	theme	elaboration	531:541	arg1	phases					513:518	early phases	507:518	early phases of texture elaboration	507:541	Identification of key events and their kinetics with regard to tissue architecture and cell wall development can provide new insights on early phases of texture elaboration.
28224918	8	53	from	hemicelluloses	1324:1337	arg1	walls					1354:1358	tomato cell walls	1342:1358	tomato cell walls	1342:1358	Cell wall polysaccharide remodelling also concerns xyloglucans and (galacto)glucomannans, the major hemicelluloses in tomato cell walls.
28224918	9	54	theme	cell	1385:1388	arg1	polysaccharides					1395:1409	cell wall polysaccharides	1385:1409	cell wall polysaccharides	1385:1409	In situ localization of cell wall polysaccharides in pericarp tissue revealed non-ramified RG-I rich pectin and XyG at cellular junctions and in the middle lamella of young fruit.
28224918	4	55	theme	pectin	570:575	arg1	characteristics					604:618	pectin and hemicellulose chemical characteristics	570:618	characteristics	604:618	In this study, changes in pectin and hemicellulose chemical characteristics and location were investigated in the pericarp tissue of tomato (Solanum lycopersicon var Levovil) at four stages of development (7, 14 and 21day after anthesis (DPA) and mature green stages).
28224918	9	56	theme	polysaccharides	1395:1409	arg1	localization					1369:1380	In situ localization	1361:1380	In situ localization of cell wall polysaccharides in pericarp tissue	1361:1428	In situ localization of cell wall polysaccharides in pericarp tissue revealed non-ramified RG-I rich pectin and XyG at cellular junctions and in the middle lamella of young fruit.
28224918	7	57	theme	rhamnogalacturonan	1105:1122	arg1	chains					1131:1136	rhamnogalacturonan I side chains	1105:1136	rhamnogalacturonan I side chains	1105:1136	Fine tuning of rhamnogalacturonan I side chains appears to occur from the cell expansion phase until prior to the mature green stage.
28224918	4	58	theme	mature	791:796	arg1	stages					804:809	mature green stages	791:809	mature green stages	791:809	In this study, changes in pectin and hemicellulose chemical characteristics and location were investigated in the pericarp tissue of tomato (Solanum lycopersicon var Levovil) at four stages of development (7, 14 and 21day after anthesis (DPA) and mature green stages).
28224918	10	59	theme	homogalacturonan	1573:1588	arg1	DPA					1617:1619	14 DPA	1614:1619	14 DPA in the mesocarp	1614:1635	Blocks of non-methyl esterified homogalacturonan are detected as soon as 14 DPA in the mesocarp and remained restricted to cell corner and middle lamella whatever the stages.
28224918	10	59	theme	homogalacturonan	1573:1588	arg1	Blocks					1541:1546	Blocks	1541:1546	Blocks of non-methyl esterified homogalacturonan	1541:1588	Blocks of non-methyl esterified homogalacturonan are detected as soon as 14 DPA in the mesocarp and remained restricted to cell corner and middle lamella whatever the stages.
28224918	9	60	theme	pericarp	1414:1421	arg1	tissue					1423:1428	pericarp tissue	1414:1428	pericarp tissue	1414:1428	In situ localization of cell wall polysaccharides in pericarp tissue revealed non-ramified RG-I rich pectin and XyG at cellular junctions and in the middle lamella of young fruit.
28224918	11	61	from	role	1763:1766	arg1	adhesion					1798:1805	cell adhesion	1793:1805	cell adhesion	1793:1805	These results point to new questions about the role of pectin RGI and XyG in cell adhesion and its maintenance during cell expansion.
28224918	10	62	theme	non-methyl	1551:1560	arg1	homogalacturonan					1573:1588	non-methyl esterified homogalacturonan	1551:1588	non-methyl esterified homogalacturonan	1551:1588	Blocks of non-methyl esterified homogalacturonan are detected as soon as 14 DPA in the mesocarp and remained restricted to cell corner and middle lamella whatever the stages.
28224918	9	63	dep	In	1361:1362	arg1	situ					1364:1367	situ	1364:1367	situ	1364:1367	In situ localization of cell wall polysaccharides in pericarp tissue revealed non-ramified RG-I rich pectin and XyG at cellular junctions and in the middle lamella of young fruit.
28224918	4	64	theme	Solanum	685:691	arg1	Levovil					710:716	Solanum lycopersicon var Levovil	685:716	Solanum lycopersicon var Levovil	685:716	In this study, changes in pectin and hemicellulose chemical characteristics and location were investigated in the pericarp tissue of tomato (Solanum lycopersicon var Levovil) at four stages of development (7, 14 and 21day after anthesis (DPA) and mature green stages).
28224918	4	64	theme	Solanum	685:691	arg1	tomato					677:682	tomato	677:682	tomato (Solanum lycopersicon var Levovil)	677:717	In this study, changes in pectin and hemicellulose chemical characteristics and location were investigated in the pericarp tissue of tomato (Solanum lycopersicon var Levovil) at four stages of development (7, 14 and 21day after anthesis (DPA) and mature green stages).
28224918	1	65	theme	wall	131:134	arg1	architecture					136:147	cell wall architecture	126:147	cell wall architecture	126:147	Tomato fruit texture depends on histology and cell wall architecture, both under genetic and developmental controls.
28224918	2	66	theme	wall	320:323	arg1	construction					325:336	cell wall construction	315:336	cell wall construction	315:336	If ripening related cell wall modifications have been well documented with regard to softening, little is known about cell wall construction during early fruit development.
28224918	5	67	theme	cell	825:828	arg1	composition					835:845	cell wall composition	825:845	cell wall composition	825:845	Analysis of cell wall composition and polysaccharide structure revealed that both are continuously modified during fruit development.
28224918	9	68	theme	RG-I	1452:1455	arg1	pectin					1462:1467	non-ramified RG-I rich pectin	1439:1467	non-ramified RG-I rich pectin	1439:1467	In situ localization of cell wall polysaccharides in pericarp tissue revealed non-ramified RG-I rich pectin and XyG at cellular junctions and in the middle lamella of young fruit.
28224918	8	69	theme	wall	1229:1232	arg1	remodelling					1249:1259	Cell wall polysaccharide remodelling	1224:1259	Cell wall polysaccharide remodelling	1224:1259	Cell wall polysaccharide remodelling also concerns xyloglucans and (galacto)glucomannans, the major hemicelluloses in tomato cell walls.
28224918	2	70	theme	cell	217:220	arg1	modifications					227:239	ripening related cell wall modifications	200:239	ripening related cell wall modifications	200:239	If ripening related cell wall modifications have been well documented with regard to softening, little is known about cell wall construction during early fruit development.
28224918	5	71	theme	structure	866:874	arg1	Analysis					813:820	Analysis	813:820	Analysis of cell wall composition and polysaccharide structure	813:874	Analysis of cell wall composition and polysaccharide structure revealed that both are continuously modified during fruit development.
28224918	4	72	theme	var	706:708	arg1	Levovil					710:716	Solanum lycopersicon var Levovil	685:716	Solanum lycopersicon var Levovil	685:716	In this study, changes in pectin and hemicellulose chemical characteristics and location were investigated in the pericarp tissue of tomato (Solanum lycopersicon var Levovil) at four stages of development (7, 14 and 21day after anthesis (DPA) and mature green stages).
28224918	4	72	theme	var	706:708	arg1	tomato					677:682	tomato	677:682	tomato (Solanum lycopersicon var Levovil)	677:717	In this study, changes in pectin and hemicellulose chemical characteristics and location were investigated in the pericarp tissue of tomato (Solanum lycopersicon var Levovil) at four stages of development (7, 14 and 21day after anthesis (DPA) and mature green stages).
28224918	11	73	theme	pectin	1771:1776	arg1	RGI					1778:1780	pectin RGI	1771:1780	pectin RGI	1771:1780	These results point to new questions about the role of pectin RGI and XyG in cell adhesion and its maintenance during cell expansion.
28224918	11	74	theme	XyG	1786:1788	arg1	role					1763:1766	the role	1759:1766	the role of pectin RGI and XyG in cell adhesion	1759:1805	These results point to new questions about the role of pectin RGI and XyG in cell adhesion and its maintenance during cell expansion.
28224918	11	74	theme	XyG	1786:1788	arg1	maintenance					1815:1825	its maintenance	1811:1825	its maintenance during cell expansion	1811:1847	These results point to new questions about the role of pectin RGI and XyG in cell adhesion and its maintenance during cell expansion.
28224918	0	75	theme	fruit	66:70	arg1	growth					72:77	fruit growth	66:77	fruit growth	66:77	Remodeling of pectin and hemicelluloses in tomato pericarp during fruit growth.
28224918	9	76	theme	cellular	1480:1487	arg1	junctions					1489:1497	cellular junctions	1480:1497	cellular junctions	1480:1497	In situ localization of cell wall polysaccharides in pericarp tissue revealed non-ramified RG-I rich pectin and XyG at cellular junctions and in the middle lamella of young fruit.
28224918	6	77	theme	high	977:980	arg1	content					991:997	the relative high rhamnose content	964:997	the relative high rhamnose content in cell walls	964:1011	At early stages, the relative high rhamnose content in cell walls indicates a high synthesis of rhamnogalacturonan I next to homogalacturonan.
28224918	4	78	theme	tomato	677:682	arg1	tissue					667:672	the pericarp tissue	654:672	the pericarp tissue of tomato (Solanum lycopersicon var Levovil)	654:717	In this study, changes in pectin and hemicellulose chemical characteristics and location were investigated in the pericarp tissue of tomato (Solanum lycopersicon var Levovil) at four stages of development (7, 14 and 21day after anthesis (DPA) and mature green stages).
28224918	8	79	theme	galacto	1292:1298	arg1	glucomannans					1300:1311	(galacto)glucomannans	1291:1311	(galacto)glucomannans	1291:1311	Cell wall polysaccharide remodelling also concerns xyloglucans and (galacto)glucomannans, the major hemicelluloses in tomato cell walls.
28224918	11	80	theme	new	1739:1741	arg1	questions					1743:1751	new questions	1739:1751	new questions about the role of pectin RGI and XyG in cell adhesion and its maintenance during cell expansion	1739:1847	These results point to new questions about the role of pectin RGI and XyG in cell adhesion and its maintenance during cell expansion.
28224918	7	81	theme	cell	1164:1167	arg1	phase					1179:1183	the cell expansion phase	1160:1183	the cell expansion phase	1160:1183	Fine tuning of rhamnogalacturonan I side chains appears to occur from the cell expansion phase until prior to the mature green stage.
28224918	5	82	theme	fruit	928:932	arg1	development					934:944	fruit development	928:944	fruit development	928:944	Analysis of cell wall composition and polysaccharide structure revealed that both are continuously modified during fruit development.
28224918	11	83	from	maintenance	1815:1825	arg1	adhesion					1798:1805	cell adhesion	1793:1805	cell adhesion	1793:1805	These results point to new questions about the role of pectin RGI and XyG in cell adhesion and its maintenance during cell expansion.
28224918	1	84	dep	histology	112:120	arg1	both					150:153	both	150:153	both	150:153	Tomato fruit texture depends on histology and cell wall architecture, both under genetic and developmental controls.
28224918	3	85	theme	tissue	433:438	arg1	architecture					440:451	tissue architecture	433:451	tissue architecture	433:451	Identification of key events and their kinetics with regard to tissue architecture and cell wall development can provide new insights on early phases of texture elaboration.
28224918	1	86	theme	Tomato	80:85	arg1	texture					93:99	Tomato fruit texture	80:99	Tomato fruit texture	80:99	Tomato fruit texture depends on histology and cell wall architecture, both under genetic and developmental controls.
28224918	6	87	theme	high	1025:1028	arg1	synthesis					1030:1038	a high synthesis	1023:1038	a high synthesis of rhamnogalacturonan I	1023:1062	At early stages, the relative high rhamnose content in cell walls indicates a high synthesis of rhamnogalacturonan I next to homogalacturonan.
28224918	3	88	theme	key	388:390	arg1	events					392:397	key events	388:397	key events	388:397	Identification of key events and their kinetics with regard to tissue architecture and cell wall development can provide new insights on early phases of texture elaboration.
28224918	7	89	theme	chains	1131:1136	arg1	tuning					1095:1100	Fine tuning	1090:1100	Fine tuning of rhamnogalacturonan I side chains	1090:1136	Fine tuning of rhamnogalacturonan I side chains appears to occur from the cell expansion phase until prior to the mature green stage.
28224918	8	90	theme	tomato	1342:1347	arg1	walls					1354:1358	tomato cell walls	1342:1358	tomato cell walls	1342:1358	Cell wall polysaccharide remodelling also concerns xyloglucans and (galacto)glucomannans, the major hemicelluloses in tomato cell walls.
28224918	3	91	theme	wall	462:465	arg1	development					467:477	cell wall development	457:477	cell wall development	457:477	Identification of key events and their kinetics with regard to tissue architecture and cell wall development can provide new insights on early phases of texture elaboration.
28224918	7	92	theme	I	1124:1124	arg1	chains					1131:1136	rhamnogalacturonan I side chains	1105:1136	rhamnogalacturonan I side chains	1105:1136	Fine tuning of rhamnogalacturonan I side chains appears to occur from the cell expansion phase until prior to the mature green stage.
28224918	9	93	from	localization	1369:1380	arg1	tissue					1423:1428	pericarp tissue	1414:1428	pericarp tissue	1414:1428	In situ localization of cell wall polysaccharides in pericarp tissue revealed non-ramified RG-I rich pectin and XyG at cellular junctions and in the middle lamella of young fruit.
28224918	6	94	theme	cell	1002:1005	arg1	walls					1007:1011	cell walls	1002:1011	cell walls	1002:1011	At early stages, the relative high rhamnose content in cell walls indicates a high synthesis of rhamnogalacturonan I next to homogalacturonan.
28224918	3	95	theme	kinetics	409:416	arg1	Identification					370:383	Identification	370:383	Identification of key events and their kinetics with regard to tissue architecture and cell wall development	370:477	Identification of key events and their kinetics with regard to tissue architecture and cell wall development can provide new insights on early phases of texture elaboration.
28224918	2	96	theme	ripening	200:207	arg1	modifications					227:239	ripening related cell wall modifications	200:239	ripening related cell wall modifications	200:239	If ripening related cell wall modifications have been well documented with regard to softening, little is known about cell wall construction during early fruit development.
28224918	3	97	theme	new	491:493	arg1	insights					495:502	new insights	491:502	new insights on early phases of texture elaboration	491:541	Identification of key events and their kinetics with regard to tissue architecture and cell wall development can provide new insights on early phases of texture elaboration.
28224918	10	98	from	DPA	1617:1619	arg1	mesocarp					1628:1635	the mesocarp	1624:1635	the mesocarp	1624:1635	Blocks of non-methyl esterified homogalacturonan are detected as soon as 14 DPA in the mesocarp and remained restricted to cell corner and middle lamella whatever the stages.
28224918	4	99	theme	chemical	595:602	arg1	characteristics					604:618	pectin and hemicellulose chemical characteristics	570:618	characteristics	604:618	In this study, changes in pectin and hemicellulose chemical characteristics and location were investigated in the pericarp tissue of tomato (Solanum lycopersicon var Levovil) at four stages of development (7, 14 and 21day after anthesis (DPA) and mature green stages).
28224918	7	100	theme	green	1211:1215	arg1	stage					1217:1221	the mature green stage	1200:1221	the mature green stage	1200:1221	Fine tuning of rhamnogalacturonan I side chains appears to occur from the cell expansion phase until prior to the mature green stage.
28224918	2	101	theme	fruit	351:355	arg1	development					357:367	early fruit development	345:367	early fruit development	345:367	If ripening related cell wall modifications have been well documented with regard to softening, little is known about cell wall construction during early fruit development.
26234581	0	0	theme	rhizobia	87:94	arg1	isolates					75:82	four isolates	70:82	four isolates of rhizobia	70:94	Chemical and rheological properties of exopolysaccharides produced by four isolates of rhizobia.
26234581	5	1	theme	shear	724:728	arg1	property					739:746	shear thinning property	724:746	shear thinning property	724:746	All polymers showed a pseudoplastic non-Newtonian fluid behavior or shear thinning property in aqueous solutions.
26234581	4	2	theme	heteropolysaccharides	633:653	arg1	production					605:614	the production	601:614	the production of extracellular heteropolysaccharides	601:653	strain that could be explored as a possible potential source for the production of extracellular heteropolysaccharides.
26234581	9	3	theme	chemical	1242:1249	arg1	composition					1251:1261	the chemical composition	1238:1261	the chemical composition	1238:1261	These findings contribute to the understanding of the influence of the chemical composition, physical properties and biotechnology applications of rhizobial EPS solutions their bioemulsifying properties.
26234581	5	4	theme	thinning	730:737	arg1	property					739:746	shear thinning property	724:746	shear thinning property	724:746	All polymers showed a pseudoplastic non-Newtonian fluid behavior or shear thinning property in aqueous solutions.
26234581	1	5	theme	exopolysaccharides	174:191	arg1	stability					161:169	stability	161:169	stability	161:169	The rheological, physicochemical properties, emulsification and stability of exopolysaccharides (EPSs) from four rhizobia isolates (LBMP-C01, LBMP-C02, LBMP-C03 and LBMP-C04) were studied.
26234581	1	5	theme	exopolysaccharides	174:191	arg1	emulsification					142:155	emulsification	142:155	emulsification	142:155	The rheological, physicochemical properties, emulsification and stability of exopolysaccharides (EPSs) from four rhizobia isolates (LBMP-C01, LBMP-C02, LBMP-C03 and LBMP-C04) were studied.
26234581	1	5	theme	exopolysaccharides	174:191	arg1	properties					130:139	The rheological, physicochemical properties	97:139	properties	130:139	The rheological, physicochemical properties, emulsification and stability of exopolysaccharides (EPSs) from four rhizobia isolates (LBMP-C01, LBMP-C02, LBMP-C03 and LBMP-C04) were studied.
26234581	6	6	dep	effective	852:860	arg1	%					907:907	89.94%	902:907	89.94%	902:907	Among the four EPS tested against hydrocarbons, EPS LBMP-C01 was found to be more effective against hexane, olive and soybean oils (89.94%, 82.75% and 81.15%, respectively).
26234581	6	6	dep	effective	852:860	arg1	%					915:915	82.75%	910:915	82.75%	910:915	Among the four EPS tested against hydrocarbons, EPS LBMP-C01 was found to be more effective against hexane, olive and soybean oils (89.94%, 82.75% and 81.15%, respectively).
26234581	6	6	dep	effective	852:860	arg1	%					926:926	81.15%	921:926	81.15%	921:926	Among the four EPS tested against hydrocarbons, EPS LBMP-C01 was found to be more effective against hexane, olive and soybean oils (89.94%, 82.75% and 81.15%, respectively).
26234581	3	7	theme	Arachis	483:489	arg1	pintoi					491:496	Arachis pintoi	483:496	Arachis pintoi	483:496	The LBMP-C04 isolate, which presented the highest EPS production (6.63gL(-1)), was isolated from Arachis pintoi and was identified as a Rhizobium sp.
26234581	7	8	from	changes	971:977	arg1	2-11					986:989	2-11	986:989	2-11	986:989	Importantly, we found that changes in pH (2-11) and salinity (0-30%) influenced the emulsification of diesel oil by the EPSs.
26234581	7	8	from	changes	971:977	arg1	salinity					996:1003	salinity	996:1003	salinity (0-30%)	996:1011	Importantly, we found that changes in pH (2-11) and salinity (0-30%) influenced the emulsification of diesel oil by the EPSs.
26234581	7	8	from	changes	971:977	arg1	pH					982:983	pH	982:983	pH (2-11)	982:990	Importantly, we found that changes in pH (2-11) and salinity (0-30%) influenced the emulsification of diesel oil by the EPSs.
26234581	7	8	from	changes	971:977	arg1	%					1010:1010	0-30%	1006:1010	0-30%	1006:1010	Importantly, we found that changes in pH (2-11) and salinity (0-30%) influenced the emulsification of diesel oil by the EPSs.
26234581	5	9	theme	pseudoplastic	678:690	arg1	fluid					706:710	a pseudoplastic non-Newtonian fluid	676:710	a pseudoplastic non-Newtonian fluid behavior	676:719	All polymers showed a pseudoplastic non-Newtonian fluid behavior or shear thinning property in aqueous solutions.
26234581	3	10	dep	6.63gL	452:457	arg1	-1					459:460	-1	459:460	-1	459:460	The LBMP-C04 isolate, which presented the highest EPS production (6.63gL(-1)), was isolated from Arachis pintoi and was identified as a Rhizobium sp.
26234581	6	11	dep	hexane	870:875	arg1	oils					896:899	oils	896:899	oils	896:899	Among the four EPS tested against hydrocarbons, EPS LBMP-C01 was found to be more effective against hexane, olive and soybean oils (89.94%, 82.75% and 81.15%, respectively).
26234581	4	12	theme	extracellular	619:631	arg1	heteropolysaccharides					633:653	extracellular heteropolysaccharides	619:653	extracellular heteropolysaccharides	619:653	strain that could be explored as a possible potential source for the production of extracellular heteropolysaccharides.
26234581	8	13	theme	30	1147:1148	arg1	%					1149:1149	%	1149:1149	%	1149:1149	EPSLBMP-C04 presented optimal emulsification capacity at pH 10 (E24=53%) and 30% salinity (E24=27%).
26234581	1	14	from	stability	161:169	arg1	LBMP-C04					262:269	LBMP-C04	262:269	LBMP-C04	262:269	The rheological, physicochemical properties, emulsification and stability of exopolysaccharides (EPSs) from four rhizobia isolates (LBMP-C01, LBMP-C02, LBMP-C03 and LBMP-C04) were studied.
26234581	1	14	from	stability	161:169	arg1	isolates					219:226	four rhizobia isolates	205:226	four rhizobia isolates (LBMP-C01, LBMP-C02, LBMP-C03 and LBMP-C04)	205:270	The rheological, physicochemical properties, emulsification and stability of exopolysaccharides (EPSs) from four rhizobia isolates (LBMP-C01, LBMP-C02, LBMP-C03 and LBMP-C04) were studied.
26234581	1	14	from	stability	161:169	arg1	LBMP-C03					249:256	LBMP-C03	249:256	LBMP-C03	249:256	The rheological, physicochemical properties, emulsification and stability of exopolysaccharides (EPSs) from four rhizobia isolates (LBMP-C01, LBMP-C02, LBMP-C03 and LBMP-C04) were studied.
26234581	2	15	dep	range	361:365	arg1	-1					381:382	-1	381:382	-1	381:382	The EPS yields of isolates under these experimental conditions were in the range of 1.5-6.63gL(-1).
26234581	9	16	theme	biotechnology	1288:1300	arg1	applications					1302:1313	biotechnology applications	1288:1313	biotechnology applications of rhizobial EPS solutions	1288:1340	These findings contribute to the understanding of the influence of the chemical composition, physical properties and biotechnology applications of rhizobial EPS solutions their bioemulsifying properties.
26234581	9	17	theme	properties	1273:1282	arg1	influence					1225:1233	the influence	1221:1233	the influence of the chemical composition, physical properties and biotechnology applications of rhizobial EPS solutions their bioemulsifying properties	1221:1372	These findings contribute to the understanding of the influence of the chemical composition, physical properties and biotechnology applications of rhizobial EPS solutions their bioemulsifying properties.
26234581	5	18	theme	fluid	706:710	arg1	behavior					712:719	a pseudoplastic non-Newtonian fluid behavior	676:719	a pseudoplastic non-Newtonian fluid behavior	676:719	All polymers showed a pseudoplastic non-Newtonian fluid behavior or shear thinning property in aqueous solutions.
26234581	7	19	theme	oil	1053:1055	arg1	emulsification					1028:1041	the emulsification	1024:1041	the emulsification of diesel oil	1024:1055	Importantly, we found that changes in pH (2-11) and salinity (0-30%) influenced the emulsification of diesel oil by the EPSs.
26234581	4	20	theme	potential	580:588	arg1	source					590:595	a possible potential source	569:595	a possible potential source for the production of extracellular heteropolysaccharides	569:653	strain that could be explored as a possible potential source for the production of extracellular heteropolysaccharides.
26234581	4	20	theme	potential	580:588	arg1	strain					536:541	strain	536:541	strain that could be explored as a possible potential source for the production of extracellular heteropolysaccharides	536:653	strain that could be explored as a possible potential source for the production of extracellular heteropolysaccharides.
26234581	7	21	theme	diesel	1046:1051	arg1	oil					1053:1055	diesel oil	1046:1055	diesel oil	1046:1055	Importantly, we found that changes in pH (2-11) and salinity (0-30%) influenced the emulsification of diesel oil by the EPSs.
26234581	1	22	from	emulsification	142:155	arg1	LBMP-C04					262:269	LBMP-C04	262:269	LBMP-C04	262:269	The rheological, physicochemical properties, emulsification and stability of exopolysaccharides (EPSs) from four rhizobia isolates (LBMP-C01, LBMP-C02, LBMP-C03 and LBMP-C04) were studied.
26234581	1	22	from	emulsification	142:155	arg1	isolates					219:226	four rhizobia isolates	205:226	four rhizobia isolates (LBMP-C01, LBMP-C02, LBMP-C03 and LBMP-C04)	205:270	The rheological, physicochemical properties, emulsification and stability of exopolysaccharides (EPSs) from four rhizobia isolates (LBMP-C01, LBMP-C02, LBMP-C03 and LBMP-C04) were studied.
26234581	1	22	from	emulsification	142:155	arg1	LBMP-C03					249:256	LBMP-C03	249:256	LBMP-C03	249:256	The rheological, physicochemical properties, emulsification and stability of exopolysaccharides (EPSs) from four rhizobia isolates (LBMP-C01, LBMP-C02, LBMP-C03 and LBMP-C04) were studied.
26234581	2	23	theme	isolates	304:311	arg1	yields					294:299	The EPS yields	286:299	The EPS yields of isolates under these experimental conditions	286:347	The EPS yields of isolates under these experimental conditions were in the range of 1.5-6.63gL(-1).
26234581	2	24	theme	1.5-6.63gL	370:379	arg1	range					361:365	the range	357:365	the range of 1.5-6.63gL	357:379	The EPS yields of isolates under these experimental conditions were in the range of 1.5-6.63gL(-1).
26234581	0	25	theme	Chemical	0:7	arg1	properties					25:34	Chemical and rheological properties	0:34	Chemical and rheological properties of exopolysaccharides	0:56	Chemical and rheological properties of exopolysaccharides produced by four isolates of rhizobia.
26234581	9	26	dep	composition	1251:1261	arg1	properties					1363:1372	their bioemulsifying properties	1342:1372	their bioemulsifying properties	1342:1372	These findings contribute to the understanding of the influence of the chemical composition, physical properties and biotechnology applications of rhizobial EPS solutions their bioemulsifying properties.
26234581	9	27	theme	applications	1302:1313	arg1	influence					1225:1233	the influence	1221:1233	the influence of the chemical composition, physical properties and biotechnology applications of rhizobial EPS solutions their bioemulsifying properties	1221:1372	These findings contribute to the understanding of the influence of the chemical composition, physical properties and biotechnology applications of rhizobial EPS solutions their bioemulsifying properties.
26234581	1	28	from	properties	130:139	arg1	LBMP-C04					262:269	LBMP-C04	262:269	LBMP-C04	262:269	The rheological, physicochemical properties, emulsification and stability of exopolysaccharides (EPSs) from four rhizobia isolates (LBMP-C01, LBMP-C02, LBMP-C03 and LBMP-C04) were studied.
26234581	1	28	from	properties	130:139	arg1	isolates					219:226	four rhizobia isolates	205:226	four rhizobia isolates (LBMP-C01, LBMP-C02, LBMP-C03 and LBMP-C04)	205:270	The rheological, physicochemical properties, emulsification and stability of exopolysaccharides (EPSs) from four rhizobia isolates (LBMP-C01, LBMP-C02, LBMP-C03 and LBMP-C04) were studied.
26234581	1	28	from	properties	130:139	arg1	LBMP-C03					249:256	LBMP-C03	249:256	LBMP-C03	249:256	The rheological, physicochemical properties, emulsification and stability of exopolysaccharides (EPSs) from four rhizobia isolates (LBMP-C01, LBMP-C02, LBMP-C03 and LBMP-C04) were studied.
26234581	1	29	theme	rheological	101:111	arg1	properties					130:139	The rheological, physicochemical properties	97:139	properties	130:139	The rheological, physicochemical properties, emulsification and stability of exopolysaccharides (EPSs) from four rhizobia isolates (LBMP-C01, LBMP-C02, LBMP-C03 and LBMP-C04) were studied.
26234581	3	30	theme	EPS	436:438	arg1	production					440:449	the highest EPS production	424:449	the highest EPS production	424:449	The LBMP-C04 isolate, which presented the highest EPS production (6.63gL(-1)), was isolated from Arachis pintoi and was identified as a Rhizobium sp.
26234581	0	31	theme	rheological	13:23	arg1	properties					25:34	Chemical and rheological properties	0:34	Chemical and rheological properties of exopolysaccharides	0:56	Chemical and rheological properties of exopolysaccharides produced by four isolates of rhizobia.
26234581	2	32	theme	EPS	290:292	arg1	yields					294:299	The EPS yields	286:299	The EPS yields of isolates under these experimental conditions	286:347	The EPS yields of isolates under these experimental conditions were in the range of 1.5-6.63gL(-1).
26234581	9	33	theme	physical	1264:1271	arg1	properties					1273:1282	physical properties	1264:1282	physical properties	1264:1282	These findings contribute to the understanding of the influence of the chemical composition, physical properties and biotechnology applications of rhizobial EPS solutions their bioemulsifying properties.
26234581	1	34	theme	rhizobia	210:217	arg1	LBMP-C04					262:269	LBMP-C04	262:269	LBMP-C04	262:269	The rheological, physicochemical properties, emulsification and stability of exopolysaccharides (EPSs) from four rhizobia isolates (LBMP-C01, LBMP-C02, LBMP-C03 and LBMP-C04) were studied.
26234581	1	34	theme	rhizobia	210:217	arg1	isolates					219:226	four rhizobia isolates	205:226	four rhizobia isolates (LBMP-C01, LBMP-C02, LBMP-C03 and LBMP-C04)	205:270	The rheological, physicochemical properties, emulsification and stability of exopolysaccharides (EPSs) from four rhizobia isolates (LBMP-C01, LBMP-C02, LBMP-C03 and LBMP-C04) were studied.
26234581	1	34	theme	rhizobia	210:217	arg1	LBMP-C03					249:256	LBMP-C03	249:256	LBMP-C03	249:256	The rheological, physicochemical properties, emulsification and stability of exopolysaccharides (EPSs) from four rhizobia isolates (LBMP-C01, LBMP-C02, LBMP-C03 and LBMP-C04) were studied.
26234581	0	35	theme	exopolysaccharides	39:56	arg1	properties					25:34	Chemical and rheological properties	0:34	Chemical and rheological properties of exopolysaccharides	0:56	Chemical and rheological properties of exopolysaccharides produced by four isolates of rhizobia.
26234581	9	36	theme	bioemulsifying	1348:1361	arg1	properties					1363:1372	their bioemulsifying properties	1342:1372	their bioemulsifying properties	1342:1372	These findings contribute to the understanding of the influence of the chemical composition, physical properties and biotechnology applications of rhizobial EPS solutions their bioemulsifying properties.
26234581	9	37	theme	EPS	1328:1330	arg1	solutions					1332:1340	rhizobial EPS solutions	1318:1340	rhizobial EPS solutions	1318:1340	These findings contribute to the understanding of the influence of the chemical composition, physical properties and biotechnology applications of rhizobial EPS solutions their bioemulsifying properties.
26234581	3	38	attach	isolated	469:476	arg2	isolate					399:405	The LBMP-C04 isolate	386:405	The LBMP-C04 isolate	386:405	The LBMP-C04 isolate, which presented the highest EPS production (6.63gL(-1)), was isolated from Arachis pintoi and was identified as a Rhizobium sp.
26234581	3	38	attach	isolated	469:476	arg1	pintoi					491:496	Arachis pintoi	483:496	Arachis pintoi	483:496	The LBMP-C04 isolate, which presented the highest EPS production (6.63gL(-1)), was isolated from Arachis pintoi and was identified as a Rhizobium sp.
26234581	3	38	attach	isolated	469:476	arg2	sp					532:533	a Rhizobium sp	520:533	a Rhizobium sp	520:533	The LBMP-C04 isolate, which presented the highest EPS production (6.63gL(-1)), was isolated from Arachis pintoi and was identified as a Rhizobium sp.
26234581	4	39	theme	possible	571:578	arg1	source					590:595	a possible potential source	569:595	a possible potential source for the production of extracellular heteropolysaccharides	569:653	strain that could be explored as a possible potential source for the production of extracellular heteropolysaccharides.
26234581	4	39	theme	possible	571:578	arg1	strain					536:541	strain	536:541	strain that could be explored as a possible potential source for the production of extracellular heteropolysaccharides	536:653	strain that could be explored as a possible potential source for the production of extracellular heteropolysaccharides.
26234581	3	40	theme	LBMP-C04	390:397	arg1	sp					532:533	a Rhizobium sp	520:533	a Rhizobium sp	520:533	The LBMP-C04 isolate, which presented the highest EPS production (6.63gL(-1)), was isolated from Arachis pintoi and was identified as a Rhizobium sp.
26234581	3	40	theme	LBMP-C04	390:397	arg1	isolate					399:405	The LBMP-C04 isolate	386:405	The LBMP-C04 isolate	386:405	The LBMP-C04 isolate, which presented the highest EPS production (6.63gL(-1)), was isolated from Arachis pintoi and was identified as a Rhizobium sp.
26234581	9	41	theme	composition	1251:1261	arg1	influence					1225:1233	the influence	1221:1233	the influence of the chemical composition, physical properties and biotechnology applications of rhizobial EPS solutions their bioemulsifying properties	1221:1372	These findings contribute to the understanding of the influence of the chemical composition, physical properties and biotechnology applications of rhizobial EPS solutions their bioemulsifying properties.
26234581	6	42	theme	EPS	818:820	arg1	LBMP-C01					822:829	EPS LBMP-C01	818:829	EPS LBMP-C01	818:829	Among the four EPS tested against hydrocarbons, EPS LBMP-C01 was found to be more effective against hexane, olive and soybean oils (89.94%, 82.75% and 81.15%, respectively).
26234581	1	43	from	isolates	219:226	arg1	stability					161:169	stability	161:169	stability	161:169	The rheological, physicochemical properties, emulsification and stability of exopolysaccharides (EPSs) from four rhizobia isolates (LBMP-C01, LBMP-C02, LBMP-C03 and LBMP-C04) were studied.
26234581	1	43	from	isolates	219:226	arg1	emulsification					142:155	emulsification	142:155	emulsification	142:155	The rheological, physicochemical properties, emulsification and stability of exopolysaccharides (EPSs) from four rhizobia isolates (LBMP-C01, LBMP-C02, LBMP-C03 and LBMP-C04) were studied.
26234581	1	43	from	isolates	219:226	arg1	EPSs					194:197	EPSs	194:197	EPSs	194:197	The rheological, physicochemical properties, emulsification and stability of exopolysaccharides (EPSs) from four rhizobia isolates (LBMP-C01, LBMP-C02, LBMP-C03 and LBMP-C04) were studied.
26234581	1	43	from	isolates	219:226	arg1	properties					130:139	The rheological, physicochemical properties	97:139	properties	130:139	The rheological, physicochemical properties, emulsification and stability of exopolysaccharides (EPSs) from four rhizobia isolates (LBMP-C01, LBMP-C02, LBMP-C03 and LBMP-C04) were studied.
26234581	1	43	from	isolates	219:226	arg1	exopolysaccharides					174:191	exopolysaccharides	174:191	exopolysaccharides (EPSs) from four rhizobia isolates (LBMP-C01, LBMP-C02, LBMP-C03 and LBMP-C04)	174:270	The rheological, physicochemical properties, emulsification and stability of exopolysaccharides (EPSs) from four rhizobia isolates (LBMP-C01, LBMP-C02, LBMP-C03 and LBMP-C04) were studied.
26234581	3	44	theme	highest	428:434	arg1	production					440:449	the highest EPS production	424:449	the highest EPS production	424:449	The LBMP-C04 isolate, which presented the highest EPS production (6.63gL(-1)), was isolated from Arachis pintoi and was identified as a Rhizobium sp.
26234581	5	45	theme	aqueous	751:757	arg1	solutions					759:767	aqueous solutions	751:767	aqueous solutions	751:767	All polymers showed a pseudoplastic non-Newtonian fluid behavior or shear thinning property in aqueous solutions.
26234581	1	46	theme	physicochemical	114:128	arg1	properties					130:139	The rheological, physicochemical properties	97:139	properties	130:139	The rheological, physicochemical properties, emulsification and stability of exopolysaccharides (EPSs) from four rhizobia isolates (LBMP-C01, LBMP-C02, LBMP-C03 and LBMP-C04) were studied.
26234581	8	47	theme	optimal	1092:1098	arg1	capacity					1115:1122	optimal emulsification capacity	1092:1122	optimal emulsification capacity	1092:1122	EPSLBMP-C04 presented optimal emulsification capacity at pH 10 (E24=53%) and 30% salinity (E24=27%).
26234581	3	48	theme	Rhizobium	522:530	arg1	sp					532:533	a Rhizobium sp	520:533	a Rhizobium sp	520:533	The LBMP-C04 isolate, which presented the highest EPS production (6.63gL(-1)), was isolated from Arachis pintoi and was identified as a Rhizobium sp.
26234581	3	48	theme	Rhizobium	522:530	arg1	isolate					399:405	The LBMP-C04 isolate	386:405	The LBMP-C04 isolate	386:405	The LBMP-C04 isolate, which presented the highest EPS production (6.63gL(-1)), was isolated from Arachis pintoi and was identified as a Rhizobium sp.
26234581	9	49	theme	rhizobial	1318:1326	arg1	solutions					1332:1340	rhizobial EPS solutions	1318:1340	rhizobial EPS solutions	1318:1340	These findings contribute to the understanding of the influence of the chemical composition, physical properties and biotechnology applications of rhizobial EPS solutions their bioemulsifying properties.
26234581	2	50	theme	experimental	325:336	arg1	conditions					338:347	these experimental conditions	319:347	these experimental conditions	319:347	The EPS yields of isolates under these experimental conditions were in the range of 1.5-6.63gL(-1).
26234581	1	51	dep	isolates	219:226	arg1	isolates					219:226	four rhizobia isolates	205:226	four rhizobia isolates (LBMP-C01, LBMP-C02, LBMP-C03 and LBMP-C04)	205:270	The rheological, physicochemical properties, emulsification and stability of exopolysaccharides (EPSs) from four rhizobia isolates (LBMP-C01, LBMP-C02, LBMP-C03 and LBMP-C04) were studied.
26234581	1	51	dep	isolates	219:226	arg1	LBMP-C04					262:269	LBMP-C04	262:269	LBMP-C04	262:269	The rheological, physicochemical properties, emulsification and stability of exopolysaccharides (EPSs) from four rhizobia isolates (LBMP-C01, LBMP-C02, LBMP-C03 and LBMP-C04) were studied.
26234581	1	51	dep	isolates	219:226	arg1	LBMP-C03					249:256	LBMP-C03	249:256	LBMP-C03	249:256	The rheological, physicochemical properties, emulsification and stability of exopolysaccharides (EPSs) from four rhizobia isolates (LBMP-C01, LBMP-C02, LBMP-C03 and LBMP-C04) were studied.
26234581	5	52	theme	non-Newtonian	692:704	arg1	fluid					706:710	a pseudoplastic non-Newtonian fluid	676:710	a pseudoplastic non-Newtonian fluid behavior	676:719	All polymers showed a pseudoplastic non-Newtonian fluid behavior or shear thinning property in aqueous solutions.
26234581	8	53	attach	presented	1082:1090	arg1	pH					1127:1128	pH 10	1127:1131	pH 10 (E24=53%)	1127:1141	EPSLBMP-C04 presented optimal emulsification capacity at pH 10 (E24=53%) and 30% salinity (E24=27%).
26234581	8	53	attach	presented	1082:1090	arg1	%					1140:1140	E24=53%	1134:1140	E24=53%	1134:1140	EPSLBMP-C04 presented optimal emulsification capacity at pH 10 (E24=53%) and 30% salinity (E24=27%).
26234581	8	53	attach	presented	1082:1090	arg1	salinity					1151:1158	30% salinity	1147:1158	30% salinity (E24=27%)	1147:1168	EPSLBMP-C04 presented optimal emulsification capacity at pH 10 (E24=53%) and 30% salinity (E24=27%).
26234581	8	53	attach	presented	1082:1090	arg1	%					1167:1167	E24=27%	1161:1167	E24=27%	1161:1167	EPSLBMP-C04 presented optimal emulsification capacity at pH 10 (E24=53%) and 30% salinity (E24=27%).
26234581	8	53	attach	presented	1082:1090	arg2	EPSLBMP-C04					1070:1080	EPSLBMP-C04	1070:1080	EPSLBMP-C04	1070:1080	EPSLBMP-C04 presented optimal emulsification capacity at pH 10 (E24=53%) and 30% salinity (E24=27%).
26234581	8	54	theme	%	1149:1149	arg1	salinity					1151:1158	30% salinity	1147:1158	30% salinity (E24=27%)	1147:1168	EPSLBMP-C04 presented optimal emulsification capacity at pH 10 (E24=53%) and 30% salinity (E24=27%).
26234581	8	54	theme	%	1149:1149	arg1	%					1167:1167	E24=27%	1161:1167	E24=27%	1161:1167	EPSLBMP-C04 presented optimal emulsification capacity at pH 10 (E24=53%) and 30% salinity (E24=27%).
26234581	9	55	theme	influence	1225:1233	arg1	understanding					1204:1216	the understanding	1200:1216	the understanding of the influence of the chemical composition, physical properties and biotechnology applications of rhizobial EPS solutions their bioemulsifying properties	1200:1372	These findings contribute to the understanding of the influence of the chemical composition, physical properties and biotechnology applications of rhizobial EPS solutions their bioemulsifying properties.
26234581	9	56	theme	solutions	1332:1340	arg1	composition					1251:1261	the chemical composition	1238:1261	the chemical composition	1238:1261	These findings contribute to the understanding of the influence of the chemical composition, physical properties and biotechnology applications of rhizobial EPS solutions their bioemulsifying properties.
26234581	9	56	theme	solutions	1332:1340	arg1	properties					1273:1282	physical properties	1264:1282	physical properties	1264:1282	These findings contribute to the understanding of the influence of the chemical composition, physical properties and biotechnology applications of rhizobial EPS solutions their bioemulsifying properties.
26234581	9	56	theme	solutions	1332:1340	arg1	applications					1302:1313	biotechnology applications	1288:1313	biotechnology applications of rhizobial EPS solutions	1288:1340	These findings contribute to the understanding of the influence of the chemical composition, physical properties and biotechnology applications of rhizobial EPS solutions their bioemulsifying properties.
26234581	8	57	theme	emulsification	1100:1113	arg1	capacity					1115:1122	optimal emulsification capacity	1092:1122	optimal emulsification capacity	1092:1122	EPSLBMP-C04 presented optimal emulsification capacity at pH 10 (E24=53%) and 30% salinity (E24=27%).
26234581	3	58	dep	presented	414:422	arg1	6.63gL					452:457	6.63gL	452:457	6.63gL	452:457	The LBMP-C04 isolate, which presented the highest EPS production (6.63gL(-1)), was isolated from Arachis pintoi and was identified as a Rhizobium sp.
28732913	7	0	theme	nanoparticles	1008:1020	arg1	presence					978:985	The presence	974:985	The presence of respective metals nanoparticles on the composite fibers	974:1044	The presence of respective metals nanoparticles on the composite fibers was confirmed using FE-SEM, XRD and FTIR.
28732913	1	1	theme	chitosan	166:173	arg1	fibers					233:238	chitosan (CS) membrane, CS/zinc phthalocyanine (ZnPc-CS) composite fibers	166:238	fibers	233:238	In this report, we prepared chitosan (CS) membrane, CS/zinc phthalocyanine (ZnPc-CS) composite fibers and pellets as support for the synthesis of zero valent metal nanoparticles.
28732913	5	2	theme	0.1M	738:741	arg1	solution					778:785	0.1M sodium borohydride (NaBH4) aqueous solution	738:785	0.1M sodium borohydride (NaBH4) aqueous solution	738:785	The metal ions adsorbed ZnPc-CS fibers were treated with 0.1M sodium borohydride (NaBH4) aqueous solution for conversion of metal ions into nanoparticles.
28732913	6	3	theme	preparation	870:880	arg1	process					882:888	water based in-situ preparation process	850:888	water based in-situ preparation process	850:888	Thus, through water based in-situ preparation process, metals nanoparticles loaded on ZnPc-CS composite fibers and pellets were achieved.
28732913	8	4	theme	reduction	1198:1206	arg1	reactions					1208:1216	the reduction reactions	1194:1216	the reduction reactions of 4-nitrophenol (4-NP), methyl orange (MO) and cango red (CR)	1194:1279	Moreover, we determined that these composites exhibit excellent catalytic efficiency and recyclability in the reduction reactions of 4-nitrophenol (4-NP), methyl orange (MO) and cango red (CR).
28732913	0	5	theme	organic	118:124	arg1	pollutants					126:135	organic pollutants	118:135	organic pollutants	118:135	Anti-bacterial chitosan/zinc phthalocyanine fibers supported metallic and bimetallic nanoparticles for the removal of organic pollutants.
28732913	2	6	from	solution	415:422	arg1	%					399:399	5wt%	396:399	5wt% of ZnPc in CS solution	396:422	The composite fibers and pellets of ZnPc-CS were prepared by simply dispersing 5wt% of ZnPc in CS solution.
28732913	2	6	from	solution	415:422	arg1	ZnPc					404:407	ZnPc	404:407	ZnPc in CS solution	404:422	The composite fibers and pellets of ZnPc-CS were prepared by simply dispersing 5wt% of ZnPc in CS solution.
28732913	9	7	dep	Escherichia	1388:1398	arg1	coli					1400:1403	coli	1400:1403	coli	1400:1403	In addition, these composites displayed high antibacterial activity as tested against pathogenic bacteria Escherichia coli.
28732913	3	8	theme	bimetallic	520:529	arg1	nanoparticles					543:555	metallic and bimetallic zero valent nanoparticles	507:555	metallic and bimetallic zero valent nanoparticles	507:555	ZnPc-CS composite were applied as economical host material for the development of metallic and bimetallic zero valent nanoparticles.
28732913	3	9	theme	nanoparticles	543:555	arg1	development					492:502	the development	488:502	the development of metallic and bimetallic zero valent nanoparticles	488:555	ZnPc-CS composite were applied as economical host material for the development of metallic and bimetallic zero valent nanoparticles.
28732913	9	10	theme	pathogenic	1368:1377	arg1	bacteria					1379:1386	pathogenic bacteria	1368:1386	pathogenic bacteria Escherichia coli	1368:1403	In addition, these composites displayed high antibacterial activity as tested against pathogenic bacteria Escherichia coli.
28732913	6	11	theme	in-situ	862:868	arg1	process					882:888	water based in-situ preparation process	850:888	water based in-situ preparation process	850:888	Thus, through water based in-situ preparation process, metals nanoparticles loaded on ZnPc-CS composite fibers and pellets were achieved.
28732913	6	12	theme	based	856:860	arg1	process					882:888	water based in-situ preparation process	850:888	water based in-situ preparation process	850:888	Thus, through water based in-situ preparation process, metals nanoparticles loaded on ZnPc-CS composite fibers and pellets were achieved.
28732913	4	13	theme	metal	669:673	arg1	ions					675:678	metal ions	669:678	metal ions	669:678	The composites of ZnPc-CS were put in 0.1M metals salt solutions (mono- and bi-metallic) for the adsorption of metal ions.
28732913	4	14	theme	ZnPc-CS	576:582	arg1	ZnPc-CS					576:582	ZnPc-CS	576:582	ZnPc-CS	576:582	The composites of ZnPc-CS were put in 0.1M metals salt solutions (mono- and bi-metallic) for the adsorption of metal ions.
28732913	4	14	theme	ZnPc-CS	576:582	arg1	composites					562:571	The composites	558:571	The composites of ZnPc-CS	558:582	The composites of ZnPc-CS were put in 0.1M metals salt solutions (mono- and bi-metallic) for the adsorption of metal ions.
28732913	7	15	attach	presence	978:985	arg1	fibers					1039:1044	the composite fibers	1025:1044	the composite fibers	1025:1044	The presence of respective metals nanoparticles on the composite fibers was confirmed using FE-SEM, XRD and FTIR.
28732913	7	15	attach	presence	978:985	arg2	nanoparticles					1008:1020	respective metals nanoparticles	990:1020	respective metals nanoparticles	990:1020	The presence of respective metals nanoparticles on the composite fibers was confirmed using FE-SEM, XRD and FTIR.
28732913	3	16	dep	metallic	507:514	arg1	zero					531:534	zero	531:534	zero	531:534	ZnPc-CS composite were applied as economical host material for the development of metallic and bimetallic zero valent nanoparticles.
28732913	4	17	theme	salt	608:611	arg1	solutions					613:621	0.1M metals salt solutions	596:621	0.1M metals salt solutions (mono- and bi-metallic)	596:645	The composites of ZnPc-CS were put in 0.1M metals salt solutions (mono- and bi-metallic) for the adsorption of metal ions.
28732913	3	18	theme	metallic	507:514	arg1	nanoparticles					543:555	metallic and bimetallic zero valent nanoparticles	507:555	metallic and bimetallic zero valent nanoparticles	507:555	ZnPc-CS composite were applied as economical host material for the development of metallic and bimetallic zero valent nanoparticles.
28732913	8	19	theme	4-nitrophenol	1221:1233	arg1	reactions					1208:1216	the reduction reactions	1194:1216	the reduction reactions of 4-nitrophenol (4-NP), methyl orange (MO) and cango red (CR)	1194:1279	Moreover, we determined that these composites exhibit excellent catalytic efficiency and recyclability in the reduction reactions of 4-nitrophenol (4-NP), methyl orange (MO) and cango red (CR).
28732913	0	20	theme	pollutants	126:135	arg1	removal					107:113	the removal	103:113	the removal of organic pollutants	103:135	Anti-bacterial chitosan/zinc phthalocyanine fibers supported metallic and bimetallic nanoparticles for the removal of organic pollutants.
28732913	5	21	theme	sodium	743:748	arg1	solution					778:785	0.1M sodium borohydride (NaBH4) aqueous solution	738:785	0.1M sodium borohydride (NaBH4) aqueous solution	738:785	The metal ions adsorbed ZnPc-CS fibers were treated with 0.1M sodium borohydride (NaBH4) aqueous solution for conversion of metal ions into nanoparticles.
28732913	5	22	theme	aqueous	770:776	arg1	solution					778:785	0.1M sodium borohydride (NaBH4) aqueous solution	738:785	0.1M sodium borohydride (NaBH4) aqueous solution	738:785	The metal ions adsorbed ZnPc-CS fibers were treated with 0.1M sodium borohydride (NaBH4) aqueous solution for conversion of metal ions into nanoparticles.
28732913	8	23	theme	excellent	1142:1150	arg1	efficiency					1162:1171	excellent catalytic efficiency	1142:1171	excellent catalytic efficiency	1142:1171	Moreover, we determined that these composites exhibit excellent catalytic efficiency and recyclability in the reduction reactions of 4-nitrophenol (4-NP), methyl orange (MO) and cango red (CR).
28732913	0	24	theme	chitosan/zinc	15:27	arg1	fibers					44:49	Anti-bacterial chitosan/zinc phthalocyanine fibers	0:49	Anti-bacterial chitosan/zinc phthalocyanine fibers	0:49	Anti-bacterial chitosan/zinc phthalocyanine fibers supported metallic and bimetallic nanoparticles for the removal of organic pollutants.
28732913	6	25	theme	water	850:854	arg1	process					882:888	water based in-situ preparation process	850:888	water based in-situ preparation process	850:888	Thus, through water based in-situ preparation process, metals nanoparticles loaded on ZnPc-CS composite fibers and pellets were achieved.
28732913	1	26	theme	membrane	180:187	arg1	fibers					233:238	chitosan (CS) membrane, CS/zinc phthalocyanine (ZnPc-CS) composite fibers	166:238	fibers	233:238	In this report, we prepared chitosan (CS) membrane, CS/zinc phthalocyanine (ZnPc-CS) composite fibers and pellets as support for the synthesis of zero valent metal nanoparticles.
28732913	0	27	theme	Anti-bacterial	0:13	arg1	fibers					44:49	Anti-bacterial chitosan/zinc phthalocyanine fibers	0:49	Anti-bacterial chitosan/zinc phthalocyanine fibers	0:49	Anti-bacterial chitosan/zinc phthalocyanine fibers supported metallic and bimetallic nanoparticles for the removal of organic pollutants.
28732913	2	28	theme	CS	412:413	arg1	solution					415:422	CS solution	412:422	CS solution	412:422	The composite fibers and pellets of ZnPc-CS were prepared by simply dispersing 5wt% of ZnPc in CS solution.
28732913	9	29	theme	Escherichia	1388:1398	arg1	bacteria					1379:1386	pathogenic bacteria	1368:1386	pathogenic bacteria Escherichia coli	1368:1403	In addition, these composites displayed high antibacterial activity as tested against pathogenic bacteria Escherichia coli.
28732913	3	30	theme	valent	536:541	arg1	nanoparticles					543:555	metallic and bimetallic zero valent nanoparticles	507:555	metallic and bimetallic zero valent nanoparticles	507:555	ZnPc-CS composite were applied as economical host material for the development of metallic and bimetallic zero valent nanoparticles.
28732913	2	31	theme	ZnPc	404:407	arg1	%					399:399	5wt%	396:399	5wt% of ZnPc in CS solution	396:422	The composite fibers and pellets of ZnPc-CS were prepared by simply dispersing 5wt% of ZnPc in CS solution.
28732913	2	31	theme	ZnPc	404:407	arg1	ZnPc					404:407	ZnPc	404:407	ZnPc in CS solution	404:422	The composite fibers and pellets of ZnPc-CS were prepared by simply dispersing 5wt% of ZnPc in CS solution.
28732913	0	32	theme	phthalocyanine	29:42	arg1	fibers					44:49	Anti-bacterial chitosan/zinc phthalocyanine fibers	0:49	Anti-bacterial chitosan/zinc phthalocyanine fibers	0:49	Anti-bacterial chitosan/zinc phthalocyanine fibers supported metallic and bimetallic nanoparticles for the removal of organic pollutants.
28732913	6	33	theme	composite	930:938	arg1	fibers					940:945	ZnPc-CS composite fibers	922:945	ZnPc-CS composite fibers	922:945	Thus, through water based in-situ preparation process, metals nanoparticles loaded on ZnPc-CS composite fibers and pellets were achieved.
28732913	1	34	dep	fibers	233:238	arg1	CS/zinc					190:196	chitosan (CS) membrane, CS/zinc phthalocyanine (ZnPc-CS) composite fibers	166:238	CS/zinc	190:196	In this report, we prepared chitosan (CS) membrane, CS/zinc phthalocyanine (ZnPc-CS) composite fibers and pellets as support for the synthesis of zero valent metal nanoparticles.
28732913	2	35	dep	fibers	331:336	arg1	The					317:319	The	317:319	The	317:319	The composite fibers and pellets of ZnPc-CS were prepared by simply dispersing 5wt% of ZnPc in CS solution.
28732913	1	36	theme	phthalocyanine	198:211	arg1	fibers					233:238	chitosan (CS) membrane, CS/zinc phthalocyanine (ZnPc-CS) composite fibers	166:238	fibers	233:238	In this report, we prepared chitosan (CS) membrane, CS/zinc phthalocyanine (ZnPc-CS) composite fibers and pellets as support for the synthesis of zero valent metal nanoparticles.
28732913	0	37	theme	metallic	61:68	arg1	nanoparticles					85:97	metallic and bimetallic nanoparticles	61:97	metallic and bimetallic nanoparticles for the removal of organic pollutants	61:135	Anti-bacterial chitosan/zinc phthalocyanine fibers supported metallic and bimetallic nanoparticles for the removal of organic pollutants.
28732913	6	38	theme	ZnPc-CS	922:928	arg1	fibers					940:945	ZnPc-CS composite fibers	922:945	ZnPc-CS composite fibers	922:945	Thus, through water based in-situ preparation process, metals nanoparticles loaded on ZnPc-CS composite fibers and pellets were achieved.
28732913	7	39	theme	composite	1029:1037	arg1	fibers					1039:1044	the composite fibers	1025:1044	the composite fibers	1025:1044	The presence of respective metals nanoparticles on the composite fibers was confirmed using FE-SEM, XRD and FTIR.
28732913	5	40	theme	metal	805:809	arg1	ions					811:814	metal ions	805:814	metal ions	805:814	The metal ions adsorbed ZnPc-CS fibers were treated with 0.1M sodium borohydride (NaBH4) aqueous solution for conversion of metal ions into nanoparticles.
28732913	3	41	theme	ZnPc-CS	425:431	arg1	composite					433:441	ZnPc-CS composite	425:441	ZnPc-CS composite	425:441	ZnPc-CS composite were applied as economical host material for the development of metallic and bimetallic zero valent nanoparticles.
28732913	3	41	theme	ZnPc-CS	425:431	arg1	material					475:482	economical host material	459:482	economical host material for the development of metallic and bimetallic zero valent nanoparticles	459:555	ZnPc-CS composite were applied as economical host material for the development of metallic and bimetallic zero valent nanoparticles.
28732913	5	42	theme	borohydride	750:760	arg1	solution					778:785	0.1M sodium borohydride (NaBH4) aqueous solution	738:785	0.1M sodium borohydride (NaBH4) aqueous solution	738:785	The metal ions adsorbed ZnPc-CS fibers were treated with 0.1M sodium borohydride (NaBH4) aqueous solution for conversion of metal ions into nanoparticles.
28732913	1	43	theme	valent	289:294	arg1	nanoparticles					302:314	zero valent metal nanoparticles	284:314	zero valent metal nanoparticles	284:314	In this report, we prepared chitosan (CS) membrane, CS/zinc phthalocyanine (ZnPc-CS) composite fibers and pellets as support for the synthesis of zero valent metal nanoparticles.
28732913	5	44	theme	metal	685:689	arg1	ZnPc-CS					705:711	ZnPc-CS	705:711	ZnPc-CS	705:711	The metal ions adsorbed ZnPc-CS fibers were treated with 0.1M sodium borohydride (NaBH4) aqueous solution for conversion of metal ions into nanoparticles.
28732913	5	44	theme	metal	685:689	arg1	ions					691:694	The metal ions	681:694	The metal ions adsorbed ZnPc-CS fibers	681:718	The metal ions adsorbed ZnPc-CS fibers were treated with 0.1M sodium borohydride (NaBH4) aqueous solution for conversion of metal ions into nanoparticles.
28732913	5	44	theme	metal	685:689	arg1	fibers					713:718	fibers	713:718	fibers	713:718	The metal ions adsorbed ZnPc-CS fibers were treated with 0.1M sodium borohydride (NaBH4) aqueous solution for conversion of metal ions into nanoparticles.
28732913	8	45	theme	orange	1250:1255	arg1	reactions					1208:1216	the reduction reactions	1194:1216	the reduction reactions of 4-nitrophenol (4-NP), methyl orange (MO) and cango red (CR)	1194:1279	Moreover, we determined that these composites exhibit excellent catalytic efficiency and recyclability in the reduction reactions of 4-nitrophenol (4-NP), methyl orange (MO) and cango red (CR).
28732913	1	46	theme	ZnPc-CS	214:220	arg1	fibers					233:238	chitosan (CS) membrane, CS/zinc phthalocyanine (ZnPc-CS) composite fibers	166:238	fibers	233:238	In this report, we prepared chitosan (CS) membrane, CS/zinc phthalocyanine (ZnPc-CS) composite fibers and pellets as support for the synthesis of zero valent metal nanoparticles.
28732913	0	47	theme	bimetallic	74:83	arg1	nanoparticles					85:97	metallic and bimetallic nanoparticles	61:97	metallic and bimetallic nanoparticles for the removal of organic pollutants	61:135	Anti-bacterial chitosan/zinc phthalocyanine fibers supported metallic and bimetallic nanoparticles for the removal of organic pollutants.
28732913	1	48	theme	metal	296:300	arg1	nanoparticles					302:314	zero valent metal nanoparticles	284:314	zero valent metal nanoparticles	284:314	In this report, we prepared chitosan (CS) membrane, CS/zinc phthalocyanine (ZnPc-CS) composite fibers and pellets as support for the synthesis of zero valent metal nanoparticles.
28732913	4	49	dep	solutions	613:621	arg1	bi-metallic					634:644	bi-metallic	634:644	bi-metallic	634:644	The composites of ZnPc-CS were put in 0.1M metals salt solutions (mono- and bi-metallic) for the adsorption of metal ions.
28732913	4	49	dep	solutions	613:621	arg1	mono-					624:628	mono-	624:628	mono-	624:628	The composites of ZnPc-CS were put in 0.1M metals salt solutions (mono- and bi-metallic) for the adsorption of metal ions.
28732913	7	50	theme	respective	990:999	arg1	nanoparticles					1008:1020	respective metals nanoparticles	990:1020	respective metals nanoparticles	990:1020	The presence of respective metals nanoparticles on the composite fibers was confirmed using FE-SEM, XRD and FTIR.
28732913	3	51	theme	host	470:473	arg1	composite					433:441	ZnPc-CS composite	425:441	ZnPc-CS composite	425:441	ZnPc-CS composite were applied as economical host material for the development of metallic and bimetallic zero valent nanoparticles.
28732913	3	51	theme	host	470:473	arg1	material					475:482	economical host material	459:482	economical host material for the development of metallic and bimetallic zero valent nanoparticles	459:555	ZnPc-CS composite were applied as economical host material for the development of metallic and bimetallic zero valent nanoparticles.
28732913	5	52	theme	NaBH4	763:767	arg1	solution					778:785	0.1M sodium borohydride (NaBH4) aqueous solution	738:785	0.1M sodium borohydride (NaBH4) aqueous solution	738:785	The metal ions adsorbed ZnPc-CS fibers were treated with 0.1M sodium borohydride (NaBH4) aqueous solution for conversion of metal ions into nanoparticles.
28732913	1	53	theme	nanoparticles	302:314	arg1	synthesis					271:279	the synthesis	267:279	the synthesis of zero valent metal nanoparticles	267:314	In this report, we prepared chitosan (CS) membrane, CS/zinc phthalocyanine (ZnPc-CS) composite fibers and pellets as support for the synthesis of zero valent metal nanoparticles.
28732913	9	54	theme	antibacterial	1327:1339	arg1	activity					1341:1348	high antibacterial activity	1322:1348	high antibacterial activity	1322:1348	In addition, these composites displayed high antibacterial activity as tested against pathogenic bacteria Escherichia coli.
28732913	4	55	theme	ions	675:678	arg1	adsorption					655:664	the adsorption	651:664	the adsorption of metal ions	651:678	The composites of ZnPc-CS were put in 0.1M metals salt solutions (mono- and bi-metallic) for the adsorption of metal ions.
28732913	6	56	theme	metals	891:896	arg1	nanoparticles					898:910	metals nanoparticles	891:910	metals nanoparticles loaded on ZnPc-CS composite fibers and pellets	891:957	Thus, through water based in-situ preparation process, metals nanoparticles loaded on ZnPc-CS composite fibers and pellets were achieved.
28732913	7	57	theme	metals	1001:1006	arg1	nanoparticles					1008:1020	respective metals nanoparticles	990:1020	respective metals nanoparticles	990:1020	The presence of respective metals nanoparticles on the composite fibers was confirmed using FE-SEM, XRD and FTIR.
28732913	2	58	theme	composite	321:329	arg1	fibers					331:336	fibers	331:336	fibers	331:336	The composite fibers and pellets of ZnPc-CS were prepared by simply dispersing 5wt% of ZnPc in CS solution.
28732913	9	59	theme	high	1322:1325	arg1	activity					1341:1348	high antibacterial activity	1322:1348	high antibacterial activity	1322:1348	In addition, these composites displayed high antibacterial activity as tested against pathogenic bacteria Escherichia coli.
28732913	7	60	from	presence	978:985	arg1	fibers					1039:1044	the composite fibers	1025:1044	the composite fibers	1025:1044	The presence of respective metals nanoparticles on the composite fibers was confirmed using FE-SEM, XRD and FTIR.
28732913	5	61	dep	adsorbed	696:703	arg1	ZnPc-CS					705:711	ZnPc-CS	705:711	ZnPc-CS	705:711	The metal ions adsorbed ZnPc-CS fibers were treated with 0.1M sodium borohydride (NaBH4) aqueous solution for conversion of metal ions into nanoparticles.
28732913	5	61	dep	adsorbed	696:703	arg1	ions					691:694	The metal ions	681:694	The metal ions adsorbed ZnPc-CS fibers	681:718	The metal ions adsorbed ZnPc-CS fibers were treated with 0.1M sodium borohydride (NaBH4) aqueous solution for conversion of metal ions into nanoparticles.
28732913	5	61	dep	adsorbed	696:703	arg1	fibers					713:718	fibers	713:718	fibers	713:718	The metal ions adsorbed ZnPc-CS fibers were treated with 0.1M sodium borohydride (NaBH4) aqueous solution for conversion of metal ions into nanoparticles.
28732913	8	62	theme	catalytic	1152:1160	arg1	efficiency					1162:1171	excellent catalytic efficiency	1142:1171	excellent catalytic efficiency	1142:1171	Moreover, we determined that these composites exhibit excellent catalytic efficiency and recyclability in the reduction reactions of 4-nitrophenol (4-NP), methyl orange (MO) and cango red (CR).
28732913	2	63	from	%	399:399	arg1	solution					415:422	CS solution	412:422	CS solution	412:422	The composite fibers and pellets of ZnPc-CS were prepared by simply dispersing 5wt% of ZnPc in CS solution.
28732913	3	64	theme	economical	459:468	arg1	composite					433:441	ZnPc-CS composite	425:441	ZnPc-CS composite	425:441	ZnPc-CS composite were applied as economical host material for the development of metallic and bimetallic zero valent nanoparticles.
28732913	3	64	theme	economical	459:468	arg1	material					475:482	economical host material	459:482	economical host material for the development of metallic and bimetallic zero valent nanoparticles	459:555	ZnPc-CS composite were applied as economical host material for the development of metallic and bimetallic zero valent nanoparticles.
28732913	1	65	theme	composite	223:231	arg1	fibers					233:238	chitosan (CS) membrane, CS/zinc phthalocyanine (ZnPc-CS) composite fibers	166:238	fibers	233:238	In this report, we prepared chitosan (CS) membrane, CS/zinc phthalocyanine (ZnPc-CS) composite fibers and pellets as support for the synthesis of zero valent metal nanoparticles.
28732913	5	66	theme	ions	811:814	arg1	conversion					791:800	conversion	791:800	conversion of metal ions into nanoparticles	791:833	The metal ions adsorbed ZnPc-CS fibers were treated with 0.1M sodium borohydride (NaBH4) aqueous solution for conversion of metal ions into nanoparticles.
28732913	8	67	theme	methyl	1243:1248	arg1	MO					1258:1259	MO	1258:1259	MO	1258:1259	Moreover, we determined that these composites exhibit excellent catalytic efficiency and recyclability in the reduction reactions of 4-nitrophenol (4-NP), methyl orange (MO) and cango red (CR).
28732913	8	67	theme	methyl	1243:1248	arg1	orange					1250:1255	methyl orange	1243:1255	methyl orange (MO)	1243:1260	Moreover, we determined that these composites exhibit excellent catalytic efficiency and recyclability in the reduction reactions of 4-nitrophenol (4-NP), methyl orange (MO) and cango red (CR).
28732913	8	68	theme	red	1272:1274	arg1	reactions					1208:1216	the reduction reactions	1194:1216	the reduction reactions of 4-nitrophenol (4-NP), methyl orange (MO) and cango red (CR)	1194:1279	Moreover, we determined that these composites exhibit excellent catalytic efficiency and recyclability in the reduction reactions of 4-nitrophenol (4-NP), methyl orange (MO) and cango red (CR).
28732913	4	69	theme	metals	601:606	arg1	solutions					613:621	0.1M metals salt solutions	596:621	0.1M metals salt solutions (mono- and bi-metallic)	596:645	The composites of ZnPc-CS were put in 0.1M metals salt solutions (mono- and bi-metallic) for the adsorption of metal ions.
28732913	2	70	from	ZnPc	404:407	arg1	solution					415:422	CS solution	412:422	CS solution	412:422	The composite fibers and pellets of ZnPc-CS were prepared by simply dispersing 5wt% of ZnPc in CS solution.
28732913	2	71	theme	ZnPc-CS	353:359	arg1	fibers					331:336	fibers	331:336	fibers	331:336	The composite fibers and pellets of ZnPc-CS were prepared by simply dispersing 5wt% of ZnPc in CS solution.
28732913	2	71	theme	ZnPc-CS	353:359	arg1	pellets					342:348	pellets	342:348	pellets	342:348	The composite fibers and pellets of ZnPc-CS were prepared by simply dispersing 5wt% of ZnPc in CS solution.
28732913	4	72	theme	0.1M	596:599	arg1	solutions					613:621	0.1M metals salt solutions	596:621	0.1M metals salt solutions (mono- and bi-metallic)	596:645	The composites of ZnPc-CS were put in 0.1M metals salt solutions (mono- and bi-metallic) for the adsorption of metal ions.
27262461	0	0	theme	erinaceus	100:108	arg1	SG-02					110:114	Hericium erinaceus SG-02	91:114	Hericium erinaceus SG-02	91:114	Protective Effects of Extracellular and Intracellular Polysaccharides on Hepatotoxicity by Hericium erinaceus SG-02.
27262461	2	1	theme	polysaccharides	483:497	arg1	rhamnose					509:516	rhamnose	509:516	rhamnose	509:516	By the analysis of GC, the extracellular polysaccharides (EPS) were composed of arabinose, mannose, galactose, and glucose with a ratio of 1:7:14:52, and the composition of intracellular polysaccharides (IPS) was rhamnose, xylose, mannose, galactose, and glucose with a ratio of 3:4:7:14:137.
27262461	2	1	theme	polysaccharides	483:497	arg1	composition					454:464	the composition	450:464	the composition of intracellular polysaccharides (IPS)	450:503	By the analysis of GC, the extracellular polysaccharides (EPS) were composed of arabinose, mannose, galactose, and glucose with a ratio of 1:7:14:52, and the composition of intracellular polysaccharides (IPS) was rhamnose, xylose, mannose, galactose, and glucose with a ratio of 3:4:7:14:137.
27262461	4	2	theme	aminotransferase	780:795	arg1	activities					831:840	the aspartate aminotransferase and glutamic pyruvic transaminase activities	766:840	the aspartate aminotransferase and glutamic pyruvic transaminase activities in serum	766:849	Results showed that the aspartate aminotransferase and glutamic pyruvic transaminase activities in serum were reduced by the supplement of EPS and IPS, while the blood lipid levels including cholesterol, triglyceride, and albumin were improved.
27262461	1	3	from	SG-02	215:219	arg1	polysaccharides					175:189	extracellular and intracellular polysaccharides	143:189	extracellular and intracellular polysaccharides from Hericium erinaceus SG-02	143:219	The protective effects of extracellular and intracellular polysaccharides from Hericium erinaceus SG-02 on the CCl4-induced hepatic injury of mice were investigated in this work.
27262461	1	3	from	SG-02	215:219	arg1	effects					132:138	The protective effects	117:138	The protective effects of extracellular and intracellular polysaccharides from Hericium erinaceus SG-02 on the CCl4-induced hepatic injury of mice	117:262	The protective effects of extracellular and intracellular polysaccharides from Hericium erinaceus SG-02 on the CCl4-induced hepatic injury of mice were investigated in this work.
27262461	2	4	with	galactose	536:544	arg1	ratio					566:570	a ratio	564:570	a ratio of 3:4:7:14:137	564:586	By the analysis of GC, the extracellular polysaccharides (EPS) were composed of arabinose, mannose, galactose, and glucose with a ratio of 1:7:14:52, and the composition of intracellular polysaccharides (IPS) was rhamnose, xylose, mannose, galactose, and glucose with a ratio of 3:4:7:14:137.
27262461	5	5	theme	catalase	1108:1115	arg1	activities					1117:1126	the superoxide dismutase and catalase activities	1079:1126	the superoxide dismutase and catalase activities	1079:1126	In liver tissue, the lipid peroxidation and malondialdehyde were largely decreased, and the superoxide dismutase and catalase activities were significantly increased.
27262461	0	6	theme	Hericium	91:98	arg1	SG-02					110:114	Hericium erinaceus SG-02	91:114	Hericium erinaceus SG-02	91:114	Protective Effects of Extracellular and Intracellular Polysaccharides on Hepatotoxicity by Hericium erinaceus SG-02.
27262461	0	7	from	Effects	11:17	arg1	Hepatotoxicity					73:86	Hepatotoxicity	73:86	Hepatotoxicity by Hericium erinaceus SG-02	73:114	Protective Effects of Extracellular and Intracellular Polysaccharides on Hepatotoxicity by Hericium erinaceus SG-02.
27262461	4	8	theme	aspartate	770:778	arg1	aminotransferase					780:795	aspartate aminotransferase	770:795	aspartate aminotransferase	770:795	Results showed that the aspartate aminotransferase and glutamic pyruvic transaminase activities in serum were reduced by the supplement of EPS and IPS, while the blood lipid levels including cholesterol, triglyceride, and albumin were improved.
27262461	1	9	theme	Hericium	196:203	arg1	SG-02					215:219	Hericium erinaceus SG-02	196:219	Hericium erinaceus SG-02	196:219	The protective effects of extracellular and intracellular polysaccharides from Hericium erinaceus SG-02 on the CCl4-induced hepatic injury of mice were investigated in this work.
27262461	2	10	with	glucose	551:557	arg1	ratio					566:570	a ratio	564:570	a ratio of 3:4:7:14:137	564:586	By the analysis of GC, the extracellular polysaccharides (EPS) were composed of arabinose, mannose, galactose, and glucose with a ratio of 1:7:14:52, and the composition of intracellular polysaccharides (IPS) was rhamnose, xylose, mannose, galactose, and glucose with a ratio of 3:4:7:14:137.
27262461	6	11	theme	H.	1208:1209	arg1	SG-02					1221:1225	H. erinaceus SG-02	1208:1225	H. erinaceus SG-02	1208:1225	The evidence demonstrated that the EPS and IPS of H. erinaceus SG-02 were protective for liver injury.
27262461	2	12	with	galactose	396:404	arg1	ratio					426:430	a ratio	424:430	a ratio of 1:7:14:52	424:443	By the analysis of GC, the extracellular polysaccharides (EPS) were composed of arabinose, mannose, galactose, and glucose with a ratio of 1:7:14:52, and the composition of intracellular polysaccharides (IPS) was rhamnose, xylose, mannose, galactose, and glucose with a ratio of 3:4:7:14:137.
27262461	4	13	theme	glutamic	801:808	arg1	transaminase					818:829	glutamic pyruvic transaminase	801:829	glutamic pyruvic transaminase	801:829	Results showed that the aspartate aminotransferase and glutamic pyruvic transaminase activities in serum were reduced by the supplement of EPS and IPS, while the blood lipid levels including cholesterol, triglyceride, and albumin were improved.
27262461	2	14	theme	GC	315:316	arg1	analysis					303:310	the analysis	299:310	the analysis of GC	299:316	By the analysis of GC, the extracellular polysaccharides (EPS) were composed of arabinose, mannose, galactose, and glucose with a ratio of 1:7:14:52, and the composition of intracellular polysaccharides (IPS) was rhamnose, xylose, mannose, galactose, and glucose with a ratio of 3:4:7:14:137.
27262461	6	15	dep	EPS	1193:1195	arg1	the					1189:1191	the	1189:1191	the	1189:1191	The evidence demonstrated that the EPS and IPS of H. erinaceus SG-02 were protective for liver injury.
27262461	2	16	with	mannose	387:393	arg1	ratio					426:430	a ratio	424:430	a ratio of 1:7:14:52	424:443	By the analysis of GC, the extracellular polysaccharides (EPS) were composed of arabinose, mannose, galactose, and glucose with a ratio of 1:7:14:52, and the composition of intracellular polysaccharides (IPS) was rhamnose, xylose, mannose, galactose, and glucose with a ratio of 3:4:7:14:137.
27262461	2	17	with	glucose	411:417	arg1	ratio					426:430	a ratio	424:430	a ratio of 1:7:14:52	424:443	By the analysis of GC, the extracellular polysaccharides (EPS) were composed of arabinose, mannose, galactose, and glucose with a ratio of 1:7:14:52, and the composition of intracellular polysaccharides (IPS) was rhamnose, xylose, mannose, galactose, and glucose with a ratio of 3:4:7:14:137.
27262461	7	18	theme	obvious	1348:1354	arg1	effects					1356:1362	obvious effects	1348:1362	obvious effects	1348:1362	The histopathological observations of mice liver slices indicated that EPS and IPS had obvious effects on liver protection.
27262461	3	19	theme	experimental	726:737	arg1	group					739:743	the experimental group	722:743	the experimental group	722:743	The model of hepatic injury of mice was induced by CCl4 and three tested levels (200, 400, and 800 mg/kg) of EPS and IPS were set as the experimental group.
27262461	3	19	theme	experimental	726:737	arg1	levels					662:667	three tested levels	649:667	three tested levels (200, 400, and 800 mg/kg) of EPS and IPS	649:708	The model of hepatic injury of mice was induced by CCl4 and three tested levels (200, 400, and 800 mg/kg) of EPS and IPS were set as the experimental group.
27262461	4	20	theme	EPS	885:887	arg1	supplement					871:880	the supplement	867:880	the supplement of EPS and IPS	867:895	Results showed that the aspartate aminotransferase and glutamic pyruvic transaminase activities in serum were reduced by the supplement of EPS and IPS, while the blood lipid levels including cholesterol, triglyceride, and albumin were improved.
27262461	1	21	theme	erinaceus	205:213	arg1	SG-02					215:219	Hericium erinaceus SG-02	196:219	Hericium erinaceus SG-02	196:219	The protective effects of extracellular and intracellular polysaccharides from Hericium erinaceus SG-02 on the CCl4-induced hepatic injury of mice were investigated in this work.
27262461	4	22	theme	lipid	914:918	arg1	albumin					968:974	albumin	968:974	albumin	968:974	Results showed that the aspartate aminotransferase and glutamic pyruvic transaminase activities in serum were reduced by the supplement of EPS and IPS, while the blood lipid levels including cholesterol, triglyceride, and albumin were improved.
27262461	4	22	theme	lipid	914:918	arg1	cholesterol					937:947	cholesterol	937:947	cholesterol	937:947	Results showed that the aspartate aminotransferase and glutamic pyruvic transaminase activities in serum were reduced by the supplement of EPS and IPS, while the blood lipid levels including cholesterol, triglyceride, and albumin were improved.
27262461	4	22	theme	lipid	914:918	arg1	levels					920:925	the blood lipid levels	904:925	the blood lipid levels including cholesterol, triglyceride, and albumin	904:974	Results showed that the aspartate aminotransferase and glutamic pyruvic transaminase activities in serum were reduced by the supplement of EPS and IPS, while the blood lipid levels including cholesterol, triglyceride, and albumin were improved.
27262461	4	22	theme	lipid	914:918	arg1	triglyceride					950:961	triglyceride	950:961	triglyceride	950:961	Results showed that the aspartate aminotransferase and glutamic pyruvic transaminase activities in serum were reduced by the supplement of EPS and IPS, while the blood lipid levels including cholesterol, triglyceride, and albumin were improved.
27262461	7	23	theme	liver	1367:1371	arg1	protection					1373:1382	liver protection	1367:1382	liver protection	1367:1382	The histopathological observations of mice liver slices indicated that EPS and IPS had obvious effects on liver protection.
27262461	4	24	theme	blood	908:912	arg1	albumin					968:974	albumin	968:974	albumin	968:974	Results showed that the aspartate aminotransferase and glutamic pyruvic transaminase activities in serum were reduced by the supplement of EPS and IPS, while the blood lipid levels including cholesterol, triglyceride, and albumin were improved.
27262461	4	24	theme	blood	908:912	arg1	cholesterol					937:947	cholesterol	937:947	cholesterol	937:947	Results showed that the aspartate aminotransferase and glutamic pyruvic transaminase activities in serum were reduced by the supplement of EPS and IPS, while the blood lipid levels including cholesterol, triglyceride, and albumin were improved.
27262461	4	24	theme	blood	908:912	arg1	levels					920:925	the blood lipid levels	904:925	the blood lipid levels including cholesterol, triglyceride, and albumin	904:974	Results showed that the aspartate aminotransferase and glutamic pyruvic transaminase activities in serum were reduced by the supplement of EPS and IPS, while the blood lipid levels including cholesterol, triglyceride, and albumin were improved.
27262461	4	24	theme	blood	908:912	arg1	triglyceride					950:961	triglyceride	950:961	triglyceride	950:961	Results showed that the aspartate aminotransferase and glutamic pyruvic transaminase activities in serum were reduced by the supplement of EPS and IPS, while the blood lipid levels including cholesterol, triglyceride, and albumin were improved.
27262461	2	25	theme	1:7:14:52	435:443	arg1	ratio					426:430	a ratio	424:430	a ratio of 1:7:14:52	424:443	By the analysis of GC, the extracellular polysaccharides (EPS) were composed of arabinose, mannose, galactose, and glucose with a ratio of 1:7:14:52, and the composition of intracellular polysaccharides (IPS) was rhamnose, xylose, mannose, galactose, and glucose with a ratio of 3:4:7:14:137.
27262461	2	26	theme	intracellular	469:481	arg1	IPS					500:502	IPS	500:502	IPS	500:502	By the analysis of GC, the extracellular polysaccharides (EPS) were composed of arabinose, mannose, galactose, and glucose with a ratio of 1:7:14:52, and the composition of intracellular polysaccharides (IPS) was rhamnose, xylose, mannose, galactose, and glucose with a ratio of 3:4:7:14:137.
27262461	2	26	theme	intracellular	469:481	arg1	polysaccharides					483:497	intracellular polysaccharides	469:497	intracellular polysaccharides (IPS)	469:503	By the analysis of GC, the extracellular polysaccharides (EPS) were composed of arabinose, mannose, galactose, and glucose with a ratio of 1:7:14:52, and the composition of intracellular polysaccharides (IPS) was rhamnose, xylose, mannose, galactose, and glucose with a ratio of 3:4:7:14:137.
27262461	0	27	theme	Protective	0:9	arg1	Effects					11:17	Protective Effects	0:17	Protective Effects of Extracellular and Intracellular Polysaccharides on Hepatotoxicity by Hericium erinaceus SG-02	0:114	Protective Effects of Extracellular and Intracellular Polysaccharides on Hepatotoxicity by Hericium erinaceus SG-02.
27262461	2	28	theme	3:4:7:14:137	575:586	arg1	ratio					566:570	a ratio	564:570	a ratio of 3:4:7:14:137	564:586	By the analysis of GC, the extracellular polysaccharides (EPS) were composed of arabinose, mannose, galactose, and glucose with a ratio of 1:7:14:52, and the composition of intracellular polysaccharides (IPS) was rhamnose, xylose, mannose, galactose, and glucose with a ratio of 3:4:7:14:137.
27262461	1	29	from	effects	132:138	arg1	injury					249:254	the CCl4-induced hepatic injury	224:254	the CCl4-induced hepatic injury of mice	224:262	The protective effects of extracellular and intracellular polysaccharides from Hericium erinaceus SG-02 on the CCl4-induced hepatic injury of mice were investigated in this work.
27262461	1	29	from	effects	132:138	arg1	SG-02					215:219	Hericium erinaceus SG-02	196:219	Hericium erinaceus SG-02	196:219	The protective effects of extracellular and intracellular polysaccharides from Hericium erinaceus SG-02 on the CCl4-induced hepatic injury of mice were investigated in this work.
27262461	2	30	theme	extracellular	323:335	arg1	EPS					354:356	EPS	354:356	EPS	354:356	By the analysis of GC, the extracellular polysaccharides (EPS) were composed of arabinose, mannose, galactose, and glucose with a ratio of 1:7:14:52, and the composition of intracellular polysaccharides (IPS) was rhamnose, xylose, mannose, galactose, and glucose with a ratio of 3:4:7:14:137.
27262461	2	30	theme	extracellular	323:335	arg1	polysaccharides					337:351	the extracellular polysaccharides	319:351	the extracellular polysaccharides (EPS)	319:357	By the analysis of GC, the extracellular polysaccharides (EPS) were composed of arabinose, mannose, galactose, and glucose with a ratio of 1:7:14:52, and the composition of intracellular polysaccharides (IPS) was rhamnose, xylose, mannose, galactose, and glucose with a ratio of 3:4:7:14:137.
27262461	0	31	theme	Extracellular	22:34	arg1	Polysaccharides					54:68	Extracellular and Intracellular Polysaccharides	22:68	Extracellular and Intracellular Polysaccharides	22:68	Protective Effects of Extracellular and Intracellular Polysaccharides on Hepatotoxicity by Hericium erinaceus SG-02.
27262461	5	32	theme	superoxide	1083:1092	arg1	dismutase					1094:1102	superoxide dismutase	1083:1102	superoxide dismutase	1083:1102	In liver tissue, the lipid peroxidation and malondialdehyde were largely decreased, and the superoxide dismutase and catalase activities were significantly increased.
27262461	5	33	theme	liver	994:998	arg1	tissue					1000:1005	liver tissue	994:1005	liver tissue	994:1005	In liver tissue, the lipid peroxidation and malondialdehyde were largely decreased, and the superoxide dismutase and catalase activities were significantly increased.
27262461	1	34	theme	CCl4-induced	228:239	arg1	injury					249:254	the CCl4-induced hepatic injury	224:254	the CCl4-induced hepatic injury of mice	224:262	The protective effects of extracellular and intracellular polysaccharides from Hericium erinaceus SG-02 on the CCl4-induced hepatic injury of mice were investigated in this work.
27262461	5	35	theme	dismutase	1094:1102	arg1	activities					1117:1126	the superoxide dismutase and catalase activities	1079:1126	the superoxide dismutase and catalase activities	1079:1126	In liver tissue, the lipid peroxidation and malondialdehyde were largely decreased, and the superoxide dismutase and catalase activities were significantly increased.
27262461	3	36	theme	injury	610:615	arg1	model					593:597	The model	589:597	The model of hepatic injury of mice	589:623	The model of hepatic injury of mice was induced by CCl4 and three tested levels (200, 400, and 800 mg/kg) of EPS and IPS were set as the experimental group.
27262461	1	37	theme	protective	121:130	arg1	effects					132:138	The protective effects	117:138	The protective effects of extracellular and intracellular polysaccharides from Hericium erinaceus SG-02 on the CCl4-induced hepatic injury of mice	117:262	The protective effects of extracellular and intracellular polysaccharides from Hericium erinaceus SG-02 on the CCl4-induced hepatic injury of mice were investigated in this work.
27262461	1	38	theme	hepatic	241:247	arg1	injury					249:254	the CCl4-induced hepatic injury	224:254	the CCl4-induced hepatic injury of mice	224:262	The protective effects of extracellular and intracellular polysaccharides from Hericium erinaceus SG-02 on the CCl4-induced hepatic injury of mice were investigated in this work.
27262461	0	39	theme	Intracellular	40:52	arg1	Polysaccharides					54:68	Extracellular and Intracellular Polysaccharides	22:68	Extracellular and Intracellular Polysaccharides	22:68	Protective Effects of Extracellular and Intracellular Polysaccharides on Hepatotoxicity by Hericium erinaceus SG-02.
27262461	6	40	theme	liver	1247:1251	arg1	injury					1253:1258	liver injury	1247:1258	liver injury	1247:1258	The evidence demonstrated that the EPS and IPS of H. erinaceus SG-02 were protective for liver injury.
27262461	7	41	theme	slices	1310:1315	arg1	observations					1283:1294	The histopathological observations	1261:1294	The histopathological observations of mice liver slices	1261:1315	The histopathological observations of mice liver slices indicated that EPS and IPS had obvious effects on liver protection.
27262461	3	42	theme	EPS	698:700	arg1	group					739:743	the experimental group	722:743	the experimental group	722:743	The model of hepatic injury of mice was induced by CCl4 and three tested levels (200, 400, and 800 mg/kg) of EPS and IPS were set as the experimental group.
27262461	3	42	theme	EPS	698:700	arg1	levels					662:667	three tested levels	649:667	three tested levels (200, 400, and 800 mg/kg) of EPS and IPS	649:708	The model of hepatic injury of mice was induced by CCl4 and three tested levels (200, 400, and 800 mg/kg) of EPS and IPS were set as the experimental group.
27262461	5	43	theme	lipid	1012:1016	arg1	peroxidation					1018:1029	the lipid peroxidation	1008:1029	the lipid peroxidation	1008:1029	In liver tissue, the lipid peroxidation and malondialdehyde were largely decreased, and the superoxide dismutase and catalase activities were significantly increased.
27262461	3	44	dep	levels	662:667	arg1	400					675:677	400	675:677	400	675:677	The model of hepatic injury of mice was induced by CCl4 and three tested levels (200, 400, and 800 mg/kg) of EPS and IPS were set as the experimental group.
27262461	3	44	dep	levels	662:667	arg1	800 mg/kg					684:692	800 mg/kg	684:692	800 mg/kg	684:692	The model of hepatic injury of mice was induced by CCl4 and three tested levels (200, 400, and 800 mg/kg) of EPS and IPS were set as the experimental group.
27262461	4	45	theme	transaminase	818:829	arg1	activities					831:840	the aspartate aminotransferase and glutamic pyruvic transaminase activities	766:840	the aspartate aminotransferase and glutamic pyruvic transaminase activities in serum	766:849	Results showed that the aspartate aminotransferase and glutamic pyruvic transaminase activities in serum were reduced by the supplement of EPS and IPS, while the blood lipid levels including cholesterol, triglyceride, and albumin were improved.
27262461	3	46	theme	hepatic	602:608	arg1	injury					610:615	hepatic injury	602:615	hepatic injury of mice	602:623	The model of hepatic injury of mice was induced by CCl4 and three tested levels (200, 400, and 800 mg/kg) of EPS and IPS were set as the experimental group.
27262461	7	47	theme	mice	1299:1302	arg1	slices					1310:1315	mice liver slices	1299:1315	mice liver slices	1299:1315	The histopathological observations of mice liver slices indicated that EPS and IPS had obvious effects on liver protection.
27262461	0	48	theme	Polysaccharides	54:68	arg1	Effects					11:17	Protective Effects	0:17	Protective Effects of Extracellular and Intracellular Polysaccharides on Hepatotoxicity by Hericium erinaceus SG-02	0:114	Protective Effects of Extracellular and Intracellular Polysaccharides on Hepatotoxicity by Hericium erinaceus SG-02.
27262461	4	49	theme	pyruvic	810:816	arg1	transaminase					818:829	glutamic pyruvic transaminase	801:829	glutamic pyruvic transaminase	801:829	Results showed that the aspartate aminotransferase and glutamic pyruvic transaminase activities in serum were reduced by the supplement of EPS and IPS, while the blood lipid levels including cholesterol, triglyceride, and albumin were improved.
27262461	7	50	theme	liver	1304:1308	arg1	slices					1310:1315	mice liver slices	1299:1315	mice liver slices	1299:1315	The histopathological observations of mice liver slices indicated that EPS and IPS had obvious effects on liver protection.
27262461	6	51	theme	SG-02	1221:1225	arg1	IPS					1201:1203	IPS	1201:1203	IPS	1201:1203	The evidence demonstrated that the EPS and IPS of H. erinaceus SG-02 were protective for liver injury.
27262461	6	51	theme	SG-02	1221:1225	arg1	EPS					1193:1195	EPS	1193:1195	EPS	1193:1195	The evidence demonstrated that the EPS and IPS of H. erinaceus SG-02 were protective for liver injury.
27262461	4	52	from	activities	831:840	arg1	serum					845:849	serum	845:849	serum	845:849	Results showed that the aspartate aminotransferase and glutamic pyruvic transaminase activities in serum were reduced by the supplement of EPS and IPS, while the blood lipid levels including cholesterol, triglyceride, and albumin were improved.
27262461	1	53	theme	extracellular	143:155	arg1	polysaccharides					175:189	extracellular and intracellular polysaccharides	143:189	extracellular and intracellular polysaccharides from Hericium erinaceus SG-02	143:219	The protective effects of extracellular and intracellular polysaccharides from Hericium erinaceus SG-02 on the CCl4-induced hepatic injury of mice were investigated in this work.
27262461	1	54	theme	mice	259:262	arg1	injury					249:254	the CCl4-induced hepatic injury	224:254	the CCl4-induced hepatic injury of mice	224:262	The protective effects of extracellular and intracellular polysaccharides from Hericium erinaceus SG-02 on the CCl4-induced hepatic injury of mice were investigated in this work.
27262461	6	55	theme	erinaceus	1211:1219	arg1	SG-02					1221:1225	H. erinaceus SG-02	1208:1225	H. erinaceus SG-02	1208:1225	The evidence demonstrated that the EPS and IPS of H. erinaceus SG-02 were protective for liver injury.
27262461	3	56	theme	mice	620:623	arg1	injury					610:615	hepatic injury	602:615	hepatic injury of mice	602:623	The model of hepatic injury of mice was induced by CCl4 and three tested levels (200, 400, and 800 mg/kg) of EPS and IPS were set as the experimental group.
27262461	4	57	theme	IPS	893:895	arg1	supplement					871:880	the supplement	867:880	the supplement of EPS and IPS	867:895	Results showed that the aspartate aminotransferase and glutamic pyruvic transaminase activities in serum were reduced by the supplement of EPS and IPS, while the blood lipid levels including cholesterol, triglyceride, and albumin were improved.
27262461	1	58	theme	intracellular	161:173	arg1	polysaccharides					175:189	extracellular and intracellular polysaccharides	143:189	extracellular and intracellular polysaccharides from Hericium erinaceus SG-02	143:219	The protective effects of extracellular and intracellular polysaccharides from Hericium erinaceus SG-02 on the CCl4-induced hepatic injury of mice were investigated in this work.
27262461	2	59	with	mannose	527:533	arg1	ratio					566:570	a ratio	564:570	a ratio of 3:4:7:14:137	564:586	By the analysis of GC, the extracellular polysaccharides (EPS) were composed of arabinose, mannose, galactose, and glucose with a ratio of 1:7:14:52, and the composition of intracellular polysaccharides (IPS) was rhamnose, xylose, mannose, galactose, and glucose with a ratio of 3:4:7:14:137.
27262461	3	60	theme	IPS	706:708	arg1	group					739:743	the experimental group	722:743	the experimental group	722:743	The model of hepatic injury of mice was induced by CCl4 and three tested levels (200, 400, and 800 mg/kg) of EPS and IPS were set as the experimental group.
27262461	3	60	theme	IPS	706:708	arg1	levels					662:667	three tested levels	649:667	three tested levels (200, 400, and 800 mg/kg) of EPS and IPS	649:708	The model of hepatic injury of mice was induced by CCl4 and three tested levels (200, 400, and 800 mg/kg) of EPS and IPS were set as the experimental group.
27262461	7	61	theme	histopathological	1265:1281	arg1	observations					1283:1294	The histopathological observations	1261:1294	The histopathological observations of mice liver slices	1261:1315	The histopathological observations of mice liver slices indicated that EPS and IPS had obvious effects on liver protection.
27262461	3	62	theme	tested	655:660	arg1	group					739:743	the experimental group	722:743	the experimental group	722:743	The model of hepatic injury of mice was induced by CCl4 and three tested levels (200, 400, and 800 mg/kg) of EPS and IPS were set as the experimental group.
27262461	3	62	theme	tested	655:660	arg1	levels					662:667	three tested levels	649:667	three tested levels (200, 400, and 800 mg/kg) of EPS and IPS	649:708	The model of hepatic injury of mice was induced by CCl4 and three tested levels (200, 400, and 800 mg/kg) of EPS and IPS were set as the experimental group.
27262461	1	63	theme	polysaccharides	175:189	arg1	effects					132:138	The protective effects	117:138	The protective effects of extracellular and intracellular polysaccharides from Hericium erinaceus SG-02 on the CCl4-induced hepatic injury of mice	117:262	The protective effects of extracellular and intracellular polysaccharides from Hericium erinaceus SG-02 on the CCl4-induced hepatic injury of mice were investigated in this work.
27262461	7	64	contain	had	1344:1346	arg1	IPS					1340:1342	IPS	1340:1342	IPS	1340:1342	The histopathological observations of mice liver slices indicated that EPS and IPS had obvious effects on liver protection.
27262461	7	64	contain	had	1344:1346	arg2	effects					1356:1362	obvious effects	1348:1362	obvious effects	1348:1362	The histopathological observations of mice liver slices indicated that EPS and IPS had obvious effects on liver protection.
27262461	7	64	contain	had	1344:1346	arg1	EPS					1332:1334	EPS	1332:1334	EPS	1332:1334	The histopathological observations of mice liver slices indicated that EPS and IPS had obvious effects on liver protection.
26656264	8	0	theme	group	1032:1036	arg1	%					1070:1070	16%	1068:1070	16%	1068:1070	The sulfate group content of polysaccharide was 16%, and the uronic acid content was 5.88 and 8.48%.
26656264	8	0	theme	group	1032:1036	arg1	content					1038:1044	The sulfate group content	1020:1044	The sulfate group content of polysaccharide	1020:1062	The sulfate group content of polysaccharide was 16%, and the uronic acid content was 5.88 and 8.48%.
26656264	12	1	theme	S180	1450:1453	arg1	tumor					1455:1459	implanted S180 tumor	1440:1459	implanted S180 tumor	1440:1459	Different doses of PPS all could inhibit the growth of implanted S180 tumor.
26656264	5	2	theme	oxide	723:727	arg1	emission					734:741	nitric oxide (NO) emission	716:741	nitric oxide (NO) emission	716:741	The immunomodulatory activities were evaluated in vitro by detecting nitric oxide (NO) emission, neutral red uptake and macrophage proliferation.
26656264	6	3	theme	mouse	875:879	arg1	model					881:885	S180-tumor-bearing mouse model	856:885	S180-tumor-bearing mouse model	856:885	Antitumor activities of degraded fragments were detected using S180-tumor-bearing mouse model by intragastric administration.
26656264	7	4	theme	FINDINGS	923:930	arg1	polysaccharides					941:955	KEY FINDINGS Degraded polysaccharides PPS1 and PPS2	919:969	KEY FINDINGS Degraded polysaccharides PPS1 and PPS2	919:969	KEY FINDINGS Degraded polysaccharides PPS1 and PPS2 were obtained at avg-MW of 386.96 and 54.99 kDa.
26656264	7	4	theme	FINDINGS	923:930	arg1	PPS2					966:969	PPS2	966:969	PPS2	966:969	KEY FINDINGS Degraded polysaccharides PPS1 and PPS2 were obtained at avg-MW of 386.96 and 54.99 kDa.
26656264	7	4	theme	FINDINGS	923:930	arg1	PPS1					957:960	PPS1	957:960	PPS1	957:960	KEY FINDINGS Degraded polysaccharides PPS1 and PPS2 were obtained at avg-MW of 386.96 and 54.99 kDa.
26656264	5	5	theme	neutral	744:750	arg1	uptake					756:761	neutral red uptake	744:761	neutral red uptake	744:761	The immunomodulatory activities were evaluated in vitro by detecting nitric oxide (NO) emission, neutral red uptake and macrophage proliferation.
26656264	2	6	theme	Pavlova	326:332	arg1	viridis					334:340	microalgae Pavlova viridis	315:340	microalgae Pavlova viridis	315:340	Here, we investigated the physicochemical properties and bioactivities of the polysaccharide from microalgae Pavlova viridis, and indicated the structure-activity relationship.
26656264	12	7	theme	PPS	1404:1406	arg1	doses					1395:1399	Different doses	1385:1399	Different doses of PPS all	1385:1410	Different doses of PPS all could inhibit the growth of implanted S180 tumor.
26656264	14	8	theme	lymphocyte	1624:1633	arg1	proliferation					1635:1647	lymphocyte proliferation	1624:1647	lymphocyte proliferation	1624:1647	Different-MW PPS significantly increased lymphocyte proliferation.
26656264	13	9	theme	CTX-treated	1564:1574	arg1	group					1576:1580	CTX-treated group	1564:1580	CTX-treated group	1564:1580	At dose of 200 mg/kg/day, the tumor inhibition rate of PPS2 was 57.06%, about 23.6% less than that of CTX-treated group.
26656264	16	10	theme	immune	1855:1860	arg1	response					1862:1869	immune response	1855:1869	immune response	1855:1869	SIGNIFICANCE The polysaccharides of Pavlova viridis had potential antitumor activities by improving immune response.
26656264	16	11	dep	SIGNIFICANCE	1755:1766	arg1	polysaccharides					1772:1786	The polysaccharides	1768:1786	SIGNIFICANCE The polysaccharides of Pavlova viridis	1755:1805	SIGNIFICANCE The polysaccharides of Pavlova viridis had potential antitumor activities by improving immune response.
26656264	14	12	theme	Different-MW	1583:1594	arg1	PPS					1596:1598	Different-MW PPS	1583:1598	Different-MW PPS	1583:1598	Different-MW PPS significantly increased lymphocyte proliferation.
26656264	0	13	theme	polysaccharide	73:86	arg1	immunomodulation					28:43	immunomodulation	28:43	immunomodulation	28:43	Physicochemical properties, immunomodulation and antitumor activities of polysaccharide from Pavlova viridis.
26656264	0	13	theme	polysaccharide	73:86	arg1	activities					59:68	antitumor activities	49:68	antitumor activities	49:68	Physicochemical properties, immunomodulation and antitumor activities of polysaccharide from Pavlova viridis.
26656264	0	13	theme	polysaccharide	73:86	arg1	properties					16:25	Physicochemical properties	0:25	Physicochemical properties	0:25	Physicochemical properties, immunomodulation and antitumor activities of polysaccharide from Pavlova viridis.
26656264	13	14	theme	less	1546:1549	arg1	%					1531:1531	57.06%	1526:1531	57.06%	1526:1531	At dose of 200 mg/kg/day, the tumor inhibition rate of PPS2 was 57.06%, about 23.6% less than that of CTX-treated group.
26656264	13	14	theme	less	1546:1549	arg1	rate					1509:1512	the tumor inhibition rate	1488:1512	the tumor inhibition rate of PPS2	1488:1520	At dose of 200 mg/kg/day, the tumor inhibition rate of PPS2 was 57.06%, about 23.6% less than that of CTX-treated group.
26656264	2	15	from	viridis	334:340	arg1	bioactivities					274:286	bioactivities	274:286	bioactivities	274:286	Here, we investigated the physicochemical properties and bioactivities of the polysaccharide from microalgae Pavlova viridis, and indicated the structure-activity relationship.
26656264	2	15	from	viridis	334:340	arg1	polysaccharide					295:308	the polysaccharide	291:308	the polysaccharide from microalgae Pavlova viridis	291:340	Here, we investigated the physicochemical properties and bioactivities of the polysaccharide from microalgae Pavlova viridis, and indicated the structure-activity relationship.
26656264	2	15	from	viridis	334:340	arg1	properties					259:268	the physicochemical properties	239:268	the physicochemical properties	239:268	Here, we investigated the physicochemical properties and bioactivities of the polysaccharide from microalgae Pavlova viridis, and indicated the structure-activity relationship.
26656264	11	16	theme	Low-MW	1322:1327	arg1	fragments					1329:1337	Low-MW fragments	1322:1337	Low-MW fragments	1322:1337	PPS2 in Low-MW fragments had the strongest immunoenhancing activities.
26656264	15	17	theme	CTX-treated	1736:1746	arg1	group					1748:1752	CTX-treated group	1736:1752	CTX-treated group	1736:1752	At 200 mg/L, the proliferation index of PPS2 was 1.37, 2.03 times higher than that of CTX-treated group.
26656264	0	18	theme	Pavlova	93:99	arg1	viridis					101:107	Pavlova viridis	93:107	Pavlova viridis	93:107	Physicochemical properties, immunomodulation and antitumor activities of polysaccharide from Pavlova viridis.
26656264	8	19	theme	uronic	1081:1086	arg1	content					1093:1099	the uronic acid content	1077:1099	the uronic acid content	1077:1099	The sulfate group content of polysaccharide was 16%, and the uronic acid content was 5.88 and 8.48%.
26656264	8	19	theme	uronic	1081:1086	arg1	5.88					1105:1108	5.88	1105:1108	5.88	1105:1108	The sulfate group content of polysaccharide was 16%, and the uronic acid content was 5.88 and 8.48%.
26656264	1	20	theme	functional	176:185	arg1	ingredients					187:197	the functional ingredients	172:197	the functional ingredients of food or drugs	172:214	AIMS Polysaccharides synthesized by microalgae can be used as the functional ingredients of food or drugs.
26656264	1	20	theme	functional	176:185	arg1	Polysaccharides					115:129	AIMS Polysaccharides	110:129	AIMS Polysaccharides synthesized by microalgae	110:155	AIMS Polysaccharides synthesized by microalgae can be used as the functional ingredients of food or drugs.
26656264	5	21	theme	macrophage	767:776	arg1	proliferation					778:790	macrophage proliferation	767:790	macrophage proliferation	767:790	The immunomodulatory activities were evaluated in vitro by detecting nitric oxide (NO) emission, neutral red uptake and macrophage proliferation.
26656264	5	22	theme	immunomodulatory	651:666	arg1	activities					668:677	The immunomodulatory activities	647:677	The immunomodulatory activities	647:677	The immunomodulatory activities were evaluated in vitro by detecting nitric oxide (NO) emission, neutral red uptake and macrophage proliferation.
26656264	0	23	from	activities	59:68	arg1	viridis					101:107	Pavlova viridis	93:107	Pavlova viridis	93:107	Physicochemical properties, immunomodulation and antitumor activities of polysaccharide from Pavlova viridis.
26656264	11	24	theme	strongest	1347:1355	arg1	activities					1373:1382	the strongest immunoenhancing activities	1343:1382	the strongest immunoenhancing activities	1343:1382	PPS2 in Low-MW fragments had the strongest immunoenhancing activities.
26656264	13	25	from	dose	1465:1468	arg1	%					1531:1531	57.06%	1526:1531	57.06%	1526:1531	At dose of 200 mg/kg/day, the tumor inhibition rate of PPS2 was 57.06%, about 23.6% less than that of CTX-treated group.
26656264	13	25	from	dose	1465:1468	arg1	rate					1509:1512	the tumor inhibition rate	1488:1512	the tumor inhibition rate of PPS2	1488:1520	At dose of 200 mg/kg/day, the tumor inhibition rate of PPS2 was 57.06%, about 23.6% less than that of CTX-treated group.
26656264	16	26	contain	had	1807:1809	arg2	activities					1831:1840	potential antitumor activities	1811:1840	potential antitumor activities	1811:1840	SIGNIFICANCE The polysaccharides of Pavlova viridis had potential antitumor activities by improving immune response.
26656264	16	26	contain	had	1807:1809	arg1	SIGNIFICANCE					1755:1766	SIGNIFICANCE	1755:1766	SIGNIFICANCE The polysaccharides of Pavlova viridis	1755:1805	SIGNIFICANCE The polysaccharides of Pavlova viridis had potential antitumor activities by improving immune response.
26656264	12	27	dep	doses	1395:1399	arg1	all					1408:1410	all	1408:1410	all	1408:1410	Different doses of PPS all could inhibit the growth of implanted S180 tumor.
26656264	7	28	theme	kDa	1015:1017	arg1	avg-MW					988:993	avg-MW	988:993	avg-MW of 386.96 and 54.99 kDa	988:1017	KEY FINDINGS Degraded polysaccharides PPS1 and PPS2 were obtained at avg-MW of 386.96 and 54.99 kDa.
26656264	11	29	contain	had	1339:1341	arg2	activities					1373:1382	the strongest immunoenhancing activities	1343:1382	the strongest immunoenhancing activities	1343:1382	PPS2 in Low-MW fragments had the strongest immunoenhancing activities.
26656264	11	29	contain	had	1339:1341	arg1	PPS2					1314:1317	PPS2	1314:1317	PPS2 in Low-MW fragments	1314:1337	PPS2 in Low-MW fragments had the strongest immunoenhancing activities.
26656264	0	30	theme	Physicochemical	0:14	arg1	properties					16:25	Physicochemical properties	0:25	Physicochemical properties	0:25	Physicochemical properties, immunomodulation and antitumor activities of polysaccharide from Pavlova viridis.
26656264	8	31	theme	8.48	1114:1117	arg1	%					1118:1118	8.48%	1114:1118	8.48%	1114:1118	The sulfate group content of polysaccharide was 16%, and the uronic acid content was 5.88 and 8.48%.
26656264	1	32	theme	AIMS	110:113	arg1	ingredients					187:197	the functional ingredients	172:197	the functional ingredients of food or drugs	172:214	AIMS Polysaccharides synthesized by microalgae can be used as the functional ingredients of food or drugs.
26656264	1	32	theme	AIMS	110:113	arg1	Polysaccharides					115:129	AIMS Polysaccharides	110:129	AIMS Polysaccharides synthesized by microalgae	110:155	AIMS Polysaccharides synthesized by microalgae can be used as the functional ingredients of food or drugs.
26656264	13	33	theme	inhibition	1498:1507	arg1	%					1531:1531	57.06%	1526:1531	57.06%	1526:1531	At dose of 200 mg/kg/day, the tumor inhibition rate of PPS2 was 57.06%, about 23.6% less than that of CTX-treated group.
26656264	13	33	theme	inhibition	1498:1507	arg1	rate					1509:1512	the tumor inhibition rate	1488:1512	the tumor inhibition rate of PPS2	1488:1520	At dose of 200 mg/kg/day, the tumor inhibition rate of PPS2 was 57.06%, about 23.6% less than that of CTX-treated group.
26656264	16	34	theme	viridis	1799:1805	arg1	polysaccharides					1772:1786	The polysaccharides	1768:1786	SIGNIFICANCE The polysaccharides of Pavlova viridis	1755:1805	SIGNIFICANCE The polysaccharides of Pavlova viridis had potential antitumor activities by improving immune response.
26656264	11	35	from	PPS2	1314:1317	arg1	fragments					1329:1337	Low-MW fragments	1322:1337	Low-MW fragments	1322:1337	PPS2 in Low-MW fragments had the strongest immunoenhancing activities.
26656264	4	36	theme	molecular	568:576	arg1	avg-MW					586:591	avg-MW	586:591	avg-MW	586:591	The functional group content, monosaccharide composition, and average molecular weight (avg-MW) were detected by chemical or chromatographic method.
26656264	4	36	theme	molecular	568:576	arg1	weight					578:583	average molecular weight	560:583	average molecular weight (avg-MW)	560:592	The functional group content, monosaccharide composition, and average molecular weight (avg-MW) were detected by chemical or chromatographic method.
26656264	13	37	theme	mg/kg/day	1477:1485	arg1	dose					1465:1468	dose	1465:1468	dose of 200 mg/kg/day	1465:1485	At dose of 200 mg/kg/day, the tumor inhibition rate of PPS2 was 57.06%, about 23.6% less than that of CTX-treated group.
26656264	10	38	theme	degraded	1184:1191	arg1	PPSs					1193:1196	All the degraded PPSs	1176:1196	All the degraded PPSs	1176:1196	All the degraded PPSs could increase phagocytosis and proliferation of macrophages, and stimulated NO emission in a dose-dependently way.
26656264	3	39	dep	METHODS	399:405	arg1	degraded					439:446	degraded	439:446	were degraded with H2O2-vitamin C assisted by ultrasonic waves	434:495	MAIN METHODS The polysaccharides (PPS0) were degraded with H2O2-vitamin C assisted by ultrasonic waves.
26656264	6	40	theme	Antitumor	793:801	arg1	activities					803:812	Antitumor activities	793:812	Antitumor activities of degraded fragments	793:834	Antitumor activities of degraded fragments were detected using S180-tumor-bearing mouse model by intragastric administration.
26656264	12	41	theme	tumor	1455:1459	arg1	growth					1430:1435	the growth	1426:1435	the growth of implanted S180 tumor	1426:1459	Different doses of PPS all could inhibit the growth of implanted S180 tumor.
26656264	6	42	theme	degraded	817:824	arg1	fragments					826:834	degraded fragments	817:834	degraded fragments	817:834	Antitumor activities of degraded fragments were detected using S180-tumor-bearing mouse model by intragastric administration.
26656264	8	43	theme	sulfate	1024:1030	arg1	%					1070:1070	16%	1068:1070	16%	1068:1070	The sulfate group content of polysaccharide was 16%, and the uronic acid content was 5.88 and 8.48%.
26656264	8	43	theme	sulfate	1024:1030	arg1	content					1038:1044	The sulfate group content	1020:1044	The sulfate group content of polysaccharide	1020:1062	The sulfate group content of polysaccharide was 16%, and the uronic acid content was 5.88 and 8.48%.
26656264	8	44	theme	polysaccharide	1049:1062	arg1	%					1070:1070	16%	1068:1070	16%	1068:1070	The sulfate group content of polysaccharide was 16%, and the uronic acid content was 5.88 and 8.48%.
26656264	8	44	theme	polysaccharide	1049:1062	arg1	content					1038:1044	The sulfate group content	1020:1044	The sulfate group content of polysaccharide	1020:1062	The sulfate group content of polysaccharide was 16%, and the uronic acid content was 5.88 and 8.48%.
26656264	4	45	theme	chemical	611:618	arg1	method					639:644	chemical or chromatographic method	611:644	chemical or chromatographic method	611:644	The functional group content, monosaccharide composition, and average molecular weight (avg-MW) were detected by chemical or chromatographic method.
26656264	5	46	theme	nitric	716:721	arg1	NO					730:731	NO	730:731	NO	730:731	The immunomodulatory activities were evaluated in vitro by detecting nitric oxide (NO) emission, neutral red uptake and macrophage proliferation.
26656264	5	46	theme	nitric	716:721	arg1	oxide					723:727	nitric oxide	716:727	nitric oxide (NO) emission	716:741	The immunomodulatory activities were evaluated in vitro by detecting nitric oxide (NO) emission, neutral red uptake and macrophage proliferation.
26656264	2	47	theme	microalgae	315:324	arg1	viridis					334:340	microalgae Pavlova viridis	315:340	microalgae Pavlova viridis	315:340	Here, we investigated the physicochemical properties and bioactivities of the polysaccharide from microalgae Pavlova viridis, and indicated the structure-activity relationship.
26656264	10	48	theme	macrophages	1247:1257	arg1	proliferation					1230:1242	proliferation	1230:1242	proliferation	1230:1242	All the degraded PPSs could increase phagocytosis and proliferation of macrophages, and stimulated NO emission in a dose-dependently way.
26656264	10	48	theme	macrophages	1247:1257	arg1	phagocytosis					1213:1224	phagocytosis	1213:1224	phagocytosis	1213:1224	All the degraded PPSs could increase phagocytosis and proliferation of macrophages, and stimulated NO emission in a dose-dependently way.
26656264	12	49	theme	implanted	1440:1448	arg1	tumor					1455:1459	implanted S180 tumor	1440:1459	implanted S180 tumor	1440:1459	Different doses of PPS all could inhibit the growth of implanted S180 tumor.
26656264	4	50	theme	chromatographic	623:637	arg1	method					639:644	chemical or chromatographic method	611:644	chemical or chromatographic method	611:644	The functional group content, monosaccharide composition, and average molecular weight (avg-MW) were detected by chemical or chromatographic method.
26656264	6	51	theme	S180-tumor-bearing	856:873	arg1	model					881:885	S180-tumor-bearing mouse model	856:885	S180-tumor-bearing mouse model	856:885	Antitumor activities of degraded fragments were detected using S180-tumor-bearing mouse model by intragastric administration.
26656264	2	52	theme	polysaccharide	295:308	arg1	bioactivities					274:286	bioactivities	274:286	bioactivities	274:286	Here, we investigated the physicochemical properties and bioactivities of the polysaccharide from microalgae Pavlova viridis, and indicated the structure-activity relationship.
26656264	2	52	theme	polysaccharide	295:308	arg1	properties					259:268	the physicochemical properties	239:268	the physicochemical properties	239:268	Here, we investigated the physicochemical properties and bioactivities of the polysaccharide from microalgae Pavlova viridis, and indicated the structure-activity relationship.
26656264	1	53	used	used	164:167	arg2	ingredients					187:197	the functional ingredients	172:197	the functional ingredients of food or drugs	172:214	AIMS Polysaccharides synthesized by microalgae can be used as the functional ingredients of food or drugs.
26656264	1	53	used	used	164:167	arg2	Polysaccharides					115:129	AIMS Polysaccharides	110:129	AIMS Polysaccharides synthesized by microalgae	110:155	AIMS Polysaccharides synthesized by microalgae can be used as the functional ingredients of food or drugs.
26656264	7	54	theme	Degraded	932:939	arg1	polysaccharides					941:955	KEY FINDINGS Degraded polysaccharides PPS1 and PPS2	919:969	KEY FINDINGS Degraded polysaccharides PPS1 and PPS2	919:969	KEY FINDINGS Degraded polysaccharides PPS1 and PPS2 were obtained at avg-MW of 386.96 and 54.99 kDa.
26656264	7	54	theme	Degraded	932:939	arg1	PPS2					966:969	PPS2	966:969	PPS2	966:969	KEY FINDINGS Degraded polysaccharides PPS1 and PPS2 were obtained at avg-MW of 386.96 and 54.99 kDa.
26656264	7	54	theme	Degraded	932:939	arg1	PPS1					957:960	PPS1	957:960	PPS1	957:960	KEY FINDINGS Degraded polysaccharides PPS1 and PPS2 were obtained at avg-MW of 386.96 and 54.99 kDa.
26656264	1	55	theme	food	202:205	arg1	ingredients					187:197	the functional ingredients	172:197	the functional ingredients of food or drugs	172:214	AIMS Polysaccharides synthesized by microalgae can be used as the functional ingredients of food or drugs.
26656264	1	55	theme	food	202:205	arg1	Polysaccharides					115:129	AIMS Polysaccharides	110:129	AIMS Polysaccharides synthesized by microalgae	110:155	AIMS Polysaccharides synthesized by microalgae can be used as the functional ingredients of food or drugs.
26656264	4	56	theme	average	560:566	arg1	avg-MW					586:591	avg-MW	586:591	avg-MW	586:591	The functional group content, monosaccharide composition, and average molecular weight (avg-MW) were detected by chemical or chromatographic method.
26656264	4	56	theme	average	560:566	arg1	weight					578:583	average molecular weight	560:583	average molecular weight (avg-MW)	560:592	The functional group content, monosaccharide composition, and average molecular weight (avg-MW) were detected by chemical or chromatographic method.
26656264	15	57	theme	PPS2	1690:1693	arg1	higher					1716:1721	higher	1716:1721	higher	1716:1721	At 200 mg/L, the proliferation index of PPS2 was 1.37, 2.03 times higher than that of CTX-treated group.
26656264	15	57	theme	PPS2	1690:1693	arg1	index					1681:1685	the proliferation index	1663:1685	the proliferation index of PPS2	1663:1693	At 200 mg/L, the proliferation index of PPS2 was 1.37, 2.03 times higher than that of CTX-treated group.
26656264	7	58	theme	KEY	919:921	arg1	FINDINGS					923:930	KEY FINDINGS	919:930	KEY FINDINGS Degraded polysaccharides PPS1 and PPS2	919:969	KEY FINDINGS Degraded polysaccharides PPS1 and PPS2 were obtained at avg-MW of 386.96 and 54.99 kDa.
26656264	1	59	theme	drugs	210:214	arg1	ingredients					187:197	the functional ingredients	172:197	the functional ingredients of food or drugs	172:214	AIMS Polysaccharides synthesized by microalgae can be used as the functional ingredients of food or drugs.
26656264	1	59	theme	drugs	210:214	arg1	Polysaccharides					115:129	AIMS Polysaccharides	110:129	AIMS Polysaccharides synthesized by microalgae	110:155	AIMS Polysaccharides synthesized by microalgae can be used as the functional ingredients of food or drugs.
26656264	0	60	from	viridis	101:107	arg1	polysaccharide					73:86	polysaccharide	73:86	polysaccharide from Pavlova viridis	73:107	Physicochemical properties, immunomodulation and antitumor activities of polysaccharide from Pavlova viridis.
26656264	0	60	from	viridis	101:107	arg1	immunomodulation					28:43	immunomodulation	28:43	immunomodulation	28:43	Physicochemical properties, immunomodulation and antitumor activities of polysaccharide from Pavlova viridis.
26656264	0	60	from	viridis	101:107	arg1	activities					59:68	antitumor activities	49:68	antitumor activities	49:68	Physicochemical properties, immunomodulation and antitumor activities of polysaccharide from Pavlova viridis.
26656264	0	60	from	viridis	101:107	arg1	properties					16:25	Physicochemical properties	0:25	Physicochemical properties	0:25	Physicochemical properties, immunomodulation and antitumor activities of polysaccharide from Pavlova viridis.
26656264	5	61	theme	red	752:754	arg1	uptake					756:761	neutral red uptake	744:761	neutral red uptake	744:761	The immunomodulatory activities were evaluated in vitro by detecting nitric oxide (NO) emission, neutral red uptake and macrophage proliferation.
26656264	2	62	theme	physicochemical	243:257	arg1	properties					259:268	the physicochemical properties	239:268	the physicochemical properties	239:268	Here, we investigated the physicochemical properties and bioactivities of the polysaccharide from microalgae Pavlova viridis, and indicated the structure-activity relationship.
26656264	4	63	theme	functional	502:511	arg1	content					519:525	The functional group content	498:525	The functional group content	498:525	The functional group content, monosaccharide composition, and average molecular weight (avg-MW) were detected by chemical or chromatographic method.
26656264	6	64	theme	fragments	826:834	arg1	activities					803:812	Antitumor activities	793:812	Antitumor activities of degraded fragments	793:834	Antitumor activities of degraded fragments were detected using S180-tumor-bearing mouse model by intragastric administration.
26656264	0	65	from	immunomodulation	28:43	arg1	viridis					101:107	Pavlova viridis	93:107	Pavlova viridis	93:107	Physicochemical properties, immunomodulation and antitumor activities of polysaccharide from Pavlova viridis.
26656264	12	66	theme	Different	1385:1393	arg1	doses					1395:1399	Different doses	1385:1399	Different doses of PPS all	1385:1410	Different doses of PPS all could inhibit the growth of implanted S180 tumor.
26656264	8	67	theme	acid	1088:1091	arg1	content					1093:1099	the uronic acid content	1077:1099	the uronic acid content	1077:1099	The sulfate group content of polysaccharide was 16%, and the uronic acid content was 5.88 and 8.48%.
26656264	8	67	theme	acid	1088:1091	arg1	5.88					1105:1108	5.88	1105:1108	5.88	1105:1108	The sulfate group content of polysaccharide was 16%, and the uronic acid content was 5.88 and 8.48%.
26656264	0	68	from	properties	16:25	arg1	viridis					101:107	Pavlova viridis	93:107	Pavlova viridis	93:107	Physicochemical properties, immunomodulation and antitumor activities of polysaccharide from Pavlova viridis.
26656264	10	69	theme	dose-dependently	1292:1307	arg1	way					1309:1311	a dose-dependently way	1290:1311	a dose-dependently way	1290:1311	All the degraded PPSs could increase phagocytosis and proliferation of macrophages, and stimulated NO emission in a dose-dependently way.
26656264	16	70	theme	potential	1811:1819	arg1	activities					1831:1840	potential antitumor activities	1811:1840	potential antitumor activities	1811:1840	SIGNIFICANCE The polysaccharides of Pavlova viridis had potential antitumor activities by improving immune response.
26656264	17	71	theme	molecular	1916:1924	arg1	weight					1926:1931	their molecular weight	1910:1931	their molecular weight	1910:1931	Moreover, the bioactivities depend on their molecular weight.
26656264	3	72	theme	MAIN	394:397	arg1	METHODS					399:405	MAIN METHODS	394:405	MAIN METHODS The polysaccharides (PPS0) were degraded with H2O2-vitamin C assisted by ultrasonic waves.	394:496	MAIN METHODS The polysaccharides (PPS0) were degraded with H2O2-vitamin C assisted by ultrasonic waves.
26656264	4	73	theme	monosaccharide	528:541	arg1	composition					543:553	monosaccharide composition	528:553	monosaccharide composition	528:553	The functional group content, monosaccharide composition, and average molecular weight (avg-MW) were detected by chemical or chromatographic method.
26656264	11	74	theme	immunoenhancing	1357:1371	arg1	activities					1373:1382	the strongest immunoenhancing activities	1343:1382	the strongest immunoenhancing activities	1343:1382	PPS2 in Low-MW fragments had the strongest immunoenhancing activities.
26656264	3	75	theme	ultrasonic	480:489	arg1	waves					491:495	ultrasonic waves	480:495	ultrasonic waves	480:495	MAIN METHODS The polysaccharides (PPS0) were degraded with H2O2-vitamin C assisted by ultrasonic waves.
26656264	4	76	theme	group	513:517	arg1	content					519:525	The functional group content	498:525	The functional group content	498:525	The functional group content, monosaccharide composition, and average molecular weight (avg-MW) were detected by chemical or chromatographic method.
26656264	3	77	theme	assisted	468:475	arg1	C					466:466	H2O2-vitamin C	453:466	H2O2-vitamin C assisted by ultrasonic waves	453:495	MAIN METHODS The polysaccharides (PPS0) were degraded with H2O2-vitamin C assisted by ultrasonic waves.
26656264	2	78	from	properties	259:268	arg1	viridis					334:340	microalgae Pavlova viridis	315:340	microalgae Pavlova viridis	315:340	Here, we investigated the physicochemical properties and bioactivities of the polysaccharide from microalgae Pavlova viridis, and indicated the structure-activity relationship.
26656264	0	79	theme	antitumor	49:57	arg1	activities					59:68	antitumor activities	49:68	antitumor activities	49:68	Physicochemical properties, immunomodulation and antitumor activities of polysaccharide from Pavlova viridis.
26656264	16	80	theme	Pavlova	1791:1797	arg1	viridis					1799:1805	Pavlova viridis	1791:1805	Pavlova viridis	1791:1805	SIGNIFICANCE The polysaccharides of Pavlova viridis had potential antitumor activities by improving immune response.
26656264	2	81	from	bioactivities	274:286	arg1	viridis					334:340	microalgae Pavlova viridis	315:340	microalgae Pavlova viridis	315:340	Here, we investigated the physicochemical properties and bioactivities of the polysaccharide from microalgae Pavlova viridis, and indicated the structure-activity relationship.
26656264	3	82	theme	H2O2-vitamin	453:464	arg1	C					466:466	H2O2-vitamin C	453:466	H2O2-vitamin C assisted by ultrasonic waves	453:495	MAIN METHODS The polysaccharides (PPS0) were degraded with H2O2-vitamin C assisted by ultrasonic waves.
26656264	15	83	theme	proliferation	1667:1679	arg1	higher					1716:1721	higher	1716:1721	higher	1716:1721	At 200 mg/L, the proliferation index of PPS2 was 1.37, 2.03 times higher than that of CTX-treated group.
26656264	15	83	theme	proliferation	1667:1679	arg1	index					1681:1685	the proliferation index	1663:1685	the proliferation index of PPS2	1663:1693	At 200 mg/L, the proliferation index of PPS2 was 1.37, 2.03 times higher than that of CTX-treated group.
26656264	13	84	theme	PPS2	1517:1520	arg1	%					1531:1531	57.06%	1526:1531	57.06%	1526:1531	At dose of 200 mg/kg/day, the tumor inhibition rate of PPS2 was 57.06%, about 23.6% less than that of CTX-treated group.
26656264	13	84	theme	PPS2	1517:1520	arg1	rate					1509:1512	the tumor inhibition rate	1488:1512	the tumor inhibition rate of PPS2	1488:1520	At dose of 200 mg/kg/day, the tumor inhibition rate of PPS2 was 57.06%, about 23.6% less than that of CTX-treated group.
26656264	7	85	dep	polysaccharides	941:955	arg1	polysaccharides					941:955	KEY FINDINGS Degraded polysaccharides PPS1 and PPS2	919:969	KEY FINDINGS Degraded polysaccharides PPS1 and PPS2	919:969	KEY FINDINGS Degraded polysaccharides PPS1 and PPS2 were obtained at avg-MW of 386.96 and 54.99 kDa.
26656264	7	85	dep	polysaccharides	941:955	arg1	PPS2					966:969	PPS2	966:969	PPS2	966:969	KEY FINDINGS Degraded polysaccharides PPS1 and PPS2 were obtained at avg-MW of 386.96 and 54.99 kDa.
26656264	7	85	dep	polysaccharides	941:955	arg1	PPS1					957:960	PPS1	957:960	PPS1	957:960	KEY FINDINGS Degraded polysaccharides PPS1 and PPS2 were obtained at avg-MW of 386.96 and 54.99 kDa.
26656264	2	86	theme	structure-activity	361:378	arg1	relationship					380:391	the structure-activity relationship	357:391	the structure-activity relationship	357:391	Here, we investigated the physicochemical properties and bioactivities of the polysaccharide from microalgae Pavlova viridis, and indicated the structure-activity relationship.
26656264	16	87	theme	antitumor	1821:1829	arg1	activities					1831:1840	potential antitumor activities	1811:1840	potential antitumor activities	1811:1840	SIGNIFICANCE The polysaccharides of Pavlova viridis had potential antitumor activities by improving immune response.
26656264	13	88	theme	tumor	1492:1496	arg1	%					1531:1531	57.06%	1526:1531	57.06%	1526:1531	At dose of 200 mg/kg/day, the tumor inhibition rate of PPS2 was 57.06%, about 23.6% less than that of CTX-treated group.
26656264	13	88	theme	tumor	1492:1496	arg1	rate					1509:1512	the tumor inhibition rate	1488:1512	the tumor inhibition rate of PPS2	1488:1520	At dose of 200 mg/kg/day, the tumor inhibition rate of PPS2 was 57.06%, about 23.6% less than that of CTX-treated group.
26656264	6	89	theme	intragastric	890:901	arg1	administration					903:916	intragastric administration	890:916	intragastric administration	890:916	Antitumor activities of degraded fragments were detected using S180-tumor-bearing mouse model by intragastric administration.
23992941	7	0	theme	M	1066:1066	arg1	marked					1088:1093	marked	1088:1093	marked	1088:1093	The F and M cells were the most marked by the presence of large amounts of proteins observed in R cells and also inside the vacuoles of B cells.
23992941	7	0	theme	M	1066:1066	arg1	cells					1068:1072	The F and M cells	1056:1072	The F and M cells	1056:1072	The F and M cells were the most marked by the presence of large amounts of proteins observed in R cells and also inside the vacuoles of B cells.
23992941	11	1	theme	elements	1611:1618	arg1	transfer					1593:1600	transfer	1593:1600	transfer of these elements to the oocytes	1593:1633	The similarities in the histochemical composition of these hepatopancreatic cells in females with developed gonads, compared to exhausted ones, is justified by the fact that transfer of these elements to the oocytes occurs, in significant quantity, only during the initial stages of gonadal development in F. brasiliensis.
23992941	1	2	theme	developmental	269:281	arg1	stages					283:288	two different developmental stages	255:288	two different developmental stages (those with developed gonads and those with exhausted gonads)	255:350	This study provides histochemical data of the hepatopancreatic cells of adult female pink-shrimp (Farfantepenaeus brasiliensis) at two different developmental stages (those with developed gonads and those with exhausted gonads).
23992941	3	3	theme	digestive	497:505	arg1	gland					507:511	this digestive gland	492:511	this digestive gland	492:511	Five cell types were identified in this digestive gland: B (vesicular), E (embryonic), F (fibrillar), M (basal) and R (resorptive).
23992941	12	4	theme	salts	1818:1822	arg1	case					1802:1805	the case	1798:1805	the case of calcium salts	1798:1822	Also, they may be more related to the molt stage, as in the case of calcium salts.
23992941	1	5	theme	cells	187:191	arg1	data					158:161	histochemical data	144:161	histochemical data of the hepatopancreatic cells of adult female pink-shrimp	144:219	This study provides histochemical data of the hepatopancreatic cells of adult female pink-shrimp (Farfantepenaeus brasiliensis) at two different developmental stages (those with developed gonads and those with exhausted gonads).
23992941	6	6	theme	intertubular	998:1009	arg1	spaces					1011:1016	the intertubular spaces	994:1016	the intertubular spaces	994:1016	In females with exhausted gonads polysaccharides were also seen in the intertubular spaces and inside the lumina of the tubules.
23992941	10	7	theme	E	1354:1354	arg1	cells					1356:1360	The E cells	1350:1360	The E cells	1350:1360	The E cells did not react to any of the applied staining techniques.
23992941	1	8	dep	stages	283:288	arg1	those					323:327	those	323:327	those	323:327	This study provides histochemical data of the hepatopancreatic cells of adult female pink-shrimp (Farfantepenaeus brasiliensis) at two different developmental stages (those with developed gonads and those with exhausted gonads).
23992941	1	8	dep	stages	283:288	arg1	those					291:295	those	291:295	those	291:295	This study provides histochemical data of the hepatopancreatic cells of adult female pink-shrimp (Farfantepenaeus brasiliensis) at two different developmental stages (those with developed gonads and those with exhausted gonads).
23992941	1	9	theme	adult	196:200	arg1	pink-shrimp					209:219	adult female pink-shrimp	196:219	adult female pink-shrimp	196:219	This study provides histochemical data of the hepatopancreatic cells of adult female pink-shrimp (Farfantepenaeus brasiliensis) at two different developmental stages (those with developed gonads and those with exhausted gonads).
23992941	7	10	theme	B	1192:1192	arg1	cells					1194:1198	B cells	1192:1198	B cells	1192:1198	The F and M cells were the most marked by the presence of large amounts of proteins observed in R cells and also inside the vacuoles of B cells.
23992941	2	11	theme	F.	357:358	arg1	females					373:379	The F. brasiliensis females	353:379	The F. brasiliensis females	353:379	The F. brasiliensis females were collected in seawater off the Guarapari coast, Espirito Santo, Brazil.
23992941	1	12	theme	pink-shrimp	209:219	arg1	cells					187:191	the hepatopancreatic cells	166:191	the hepatopancreatic cells of adult female pink-shrimp	166:219	This study provides histochemical data of the hepatopancreatic cells of adult female pink-shrimp (Farfantepenaeus brasiliensis) at two different developmental stages (those with developed gonads and those with exhausted gonads).
23992941	11	13	from	stages	1692:1697	arg1	F.					1725:1726	F.	1725:1726	F.	1725:1726	The similarities in the histochemical composition of these hepatopancreatic cells in females with developed gonads, compared to exhausted ones, is justified by the fact that transfer of these elements to the oocytes occurs, in significant quantity, only during the initial stages of gonadal development in F. brasiliensis.
23992941	5	14	theme	B	891:891	arg1	cells					893:897	the B cells	887:897	the B cells	887:897	Acid glycoconjugates were found inside vacuoles in the R cells, while neutral polysaccharides were present in the B cells and near to the microvilli.
23992941	12	15	from	stage	1785:1789	arg1	case					1802:1805	the case	1798:1805	the case of calcium salts	1798:1822	Also, they may be more related to the molt stage, as in the case of calcium salts.
23992941	9	16	theme	F	1262:1262	arg1	cells					1264:1268	The F cells	1258:1268	The F cells	1258:1268	The F cells showed strong positive staining for lipid while the R cell only stained weakly.
23992941	8	17	located	found	1230:1234	arg2	calcium					1211:1217	The bound calcium	1201:1217	The bound calcium	1201:1217	The bound calcium was mainly found in the F and M cells.
23992941	8	17	located	found	1230:1234	arg1	cells					1251:1255	the F and M cells	1239:1255	the F and M cells	1239:1255	The bound calcium was mainly found in the F and M cells.
23992941	6	18	theme	tubules	1047:1053	arg1	lumina					1033:1038	the lumina	1029:1038	the lumina of the tubules	1029:1053	In females with exhausted gonads polysaccharides were also seen in the intertubular spaces and inside the lumina of the tubules.
23992941	4	19	theme	following	630:638	arg1	Kossa					727:731	von Kossa	723:731	von Kossa (for bound calcium)	723:751	The digestive gland was stained with the following techniques: PAS/Alcian blue (for polysaccharides), bromophenol blue (for protein), von Kossa (for bound calcium) and Baker (for lipids).
23992941	4	19	theme	following	630:638	arg1	PAS/Alcian					652:661	PAS/Alcian blue	652:666	PAS/Alcian blue (for polysaccharides)	652:688	The digestive gland was stained with the following techniques: PAS/Alcian blue (for polysaccharides), bromophenol blue (for protein), von Kossa (for bound calcium) and Baker (for lipids).
23992941	4	19	theme	following	630:638	arg1	techniques					640:649	the following techniques	626:649	the following techniques: PAS/Alcian blue (for polysaccharides), bromophenol blue (for protein), von Kossa (for bound calcium) and Baker (for lipids)	626:774	The digestive gland was stained with the following techniques: PAS/Alcian blue (for polysaccharides), bromophenol blue (for protein), von Kossa (for bound calcium) and Baker (for lipids).
23992941	4	19	theme	following	630:638	arg1	Baker					757:761	Baker	757:761	Baker (for lipids)	757:774	The digestive gland was stained with the following techniques: PAS/Alcian blue (for polysaccharides), bromophenol blue (for protein), von Kossa (for bound calcium) and Baker (for lipids).
23992941	4	19	theme	following	630:638	arg1	bromophenol					691:701	bromophenol blue	691:706	bromophenol blue (for protein)	691:720	The digestive gland was stained with the following techniques: PAS/Alcian blue (for polysaccharides), bromophenol blue (for protein), von Kossa (for bound calcium) and Baker (for lipids).
23992941	4	20	dep	techniques	640:649	arg1	PAS/Alcian					652:661	PAS/Alcian blue	652:666	PAS/Alcian blue (for polysaccharides)	652:688	The digestive gland was stained with the following techniques: PAS/Alcian blue (for polysaccharides), bromophenol blue (for protein), von Kossa (for bound calcium) and Baker (for lipids).
23992941	4	20	dep	techniques	640:649	arg1	Baker					757:761	Baker	757:761	Baker (for lipids)	757:774	The digestive gland was stained with the following techniques: PAS/Alcian blue (for polysaccharides), bromophenol blue (for protein), von Kossa (for bound calcium) and Baker (for lipids).
23992941	4	20	dep	techniques	640:649	arg1	techniques					640:649	the following techniques	626:649	the following techniques: PAS/Alcian blue (for polysaccharides), bromophenol blue (for protein), von Kossa (for bound calcium) and Baker (for lipids)	626:774	The digestive gland was stained with the following techniques: PAS/Alcian blue (for polysaccharides), bromophenol blue (for protein), von Kossa (for bound calcium) and Baker (for lipids).
23992941	4	20	dep	techniques	640:649	arg1	bromophenol					691:701	bromophenol blue	691:706	bromophenol blue (for protein)	691:720	The digestive gland was stained with the following techniques: PAS/Alcian blue (for polysaccharides), bromophenol blue (for protein), von Kossa (for bound calcium) and Baker (for lipids).
23992941	4	20	dep	techniques	640:649	arg1	Kossa					727:731	von Kossa	723:731	von Kossa (for bound calcium)	723:751	The digestive gland was stained with the following techniques: PAS/Alcian blue (for polysaccharides), bromophenol blue (for protein), von Kossa (for bound calcium) and Baker (for lipids).
23992941	7	21	theme	R	1152:1152	arg1	cells					1154:1158	R cells	1152:1158	R cells	1152:1158	The F and M cells were the most marked by the presence of large amounts of proteins observed in R cells and also inside the vacuoles of B cells.
23992941	4	22	theme	digestive	593:601	arg1	gland					603:607	The digestive gland	589:607	The digestive gland	589:607	The digestive gland was stained with the following techniques: PAS/Alcian blue (for polysaccharides), bromophenol blue (for protein), von Kossa (for bound calcium) and Baker (for lipids).
23992941	11	23	theme	cells	1495:1499	arg1	composition					1457:1467	the histochemical composition	1439:1467	the histochemical composition of these hepatopancreatic cells	1439:1499	The similarities in the histochemical composition of these hepatopancreatic cells in females with developed gonads, compared to exhausted ones, is justified by the fact that transfer of these elements to the oocytes occurs, in significant quantity, only during the initial stages of gonadal development in F. brasiliensis.
23992941	11	24	theme	gonadal	1702:1708	arg1	development					1710:1720	gonadal development	1702:1720	gonadal development in F. brasiliensis	1702:1739	The similarities in the histochemical composition of these hepatopancreatic cells in females with developed gonads, compared to exhausted ones, is justified by the fact that transfer of these elements to the oocytes occurs, in significant quantity, only during the initial stages of gonadal development in F. brasiliensis.
23992941	11	25	with	females	1504:1510	arg1	gonads					1527:1532	developed gonads	1517:1532	developed gonads	1517:1532	The similarities in the histochemical composition of these hepatopancreatic cells in females with developed gonads, compared to exhausted ones, is justified by the fact that transfer of these elements to the oocytes occurs, in significant quantity, only during the initial stages of gonadal development in F. brasiliensis.
23992941	9	26	theme	positive	1284:1291	arg1	staining					1293:1300	strong positive staining	1277:1300	strong positive staining for lipid	1277:1310	The F cells showed strong positive staining for lipid while the R cell only stained weakly.
23992941	0	27	theme	pink-shrimp	66:76	arg1	females					51:57	adult females	45:57	adult females of the pink-shrimp	45:76	Histochemical study of the hepatopancreas in adult females of the pink-shrimp Farfantepenaeus brasiliensis Latreille, 1817.
23992941	11	28	from	similarities	1423:1434	arg1	females					1504:1510	females	1504:1510	females with developed gonads	1504:1532	The similarities in the histochemical composition of these hepatopancreatic cells in females with developed gonads, compared to exhausted ones, is justified by the fact that transfer of these elements to the oocytes occurs, in significant quantity, only during the initial stages of gonadal development in F. brasiliensis.
23992941	11	28	from	similarities	1423:1434	arg1	composition					1457:1467	the histochemical composition	1439:1467	the histochemical composition of these hepatopancreatic cells	1439:1499	The similarities in the histochemical composition of these hepatopancreatic cells in females with developed gonads, compared to exhausted ones, is justified by the fact that transfer of these elements to the oocytes occurs, in significant quantity, only during the initial stages of gonadal development in F. brasiliensis.
23992941	11	29	theme	developed	1517:1525	arg1	gonads					1527:1532	developed gonads	1517:1532	developed gonads	1517:1532	The similarities in the histochemical composition of these hepatopancreatic cells in females with developed gonads, compared to exhausted ones, is justified by the fact that transfer of these elements to the oocytes occurs, in significant quantity, only during the initial stages of gonadal development in F. brasiliensis.
23992941	3	30	dep	identified	478:487	arg1	E					529:529	E	529:529	E (embryonic)	529:541	Five cell types were identified in this digestive gland: B (vesicular), E (embryonic), F (fibrillar), M (basal) and R (resorptive).
23992941	3	30	dep	identified	478:487	arg1	B					514:514	B	514:514	B (vesicular)	514:526	Five cell types were identified in this digestive gland: B (vesicular), E (embryonic), F (fibrillar), M (basal) and R (resorptive).
23992941	3	30	dep	identified	478:487	arg1	R					573:573	R	573:573	R (resorptive)	573:586	Five cell types were identified in this digestive gland: B (vesicular), E (embryonic), F (fibrillar), M (basal) and R (resorptive).
23992941	3	30	dep	identified	478:487	arg1	M					559:559	M	559:559	M (basal)	559:567	Five cell types were identified in this digestive gland: B (vesicular), E (embryonic), F (fibrillar), M (basal) and R (resorptive).
23992941	3	30	dep	identified	478:487	arg1	vesicular					517:525	vesicular	517:525	vesicular	517:525	Five cell types were identified in this digestive gland: B (vesicular), E (embryonic), F (fibrillar), M (basal) and R (resorptive).
23992941	3	30	dep	identified	478:487	arg1	resorptive					576:585	resorptive	576:585	resorptive	576:585	Five cell types were identified in this digestive gland: B (vesicular), E (embryonic), F (fibrillar), M (basal) and R (resorptive).
23992941	3	30	dep	identified	478:487	arg1	F					544:544	F	544:544	F (fibrillar)	544:556	Five cell types were identified in this digestive gland: B (vesicular), E (embryonic), F (fibrillar), M (basal) and R (resorptive).
23992941	10	31	theme	applied	1390:1396	arg1	techniques					1407:1416	the applied staining techniques	1386:1416	the applied staining techniques	1386:1416	The E cells did not react to any of the applied staining techniques.
23992941	9	32	theme	R	1322:1322	arg1	cell					1324:1327	the R cell	1318:1327	the R cell	1318:1327	The F cells showed strong positive staining for lipid while the R cell only stained weakly.
23992941	0	33	theme	Histochemical	0:12	arg1	study					14:18	Histochemical study	0:18	Histochemical study of the hepatopancreas in adult females of the pink-shrimp	0:76	Histochemical study of the hepatopancreas in adult females of the pink-shrimp Farfantepenaeus brasiliensis Latreille, 1817.
23992941	7	34	theme	proteins	1131:1138	arg1	proteins					1131:1138	proteins	1131:1138	proteins observed in R cells	1131:1158	The F and M cells were the most marked by the presence of large amounts of proteins observed in R cells and also inside the vacuoles of B cells.
23992941	7	34	theme	proteins	1131:1138	arg1	amounts					1120:1126	large amounts	1114:1126	large amounts of proteins observed in R cells	1114:1158	The F and M cells were the most marked by the presence of large amounts of proteins observed in R cells and also inside the vacuoles of B cells.
23992941	4	35	theme	blue	663:666	arg1	PAS/Alcian					652:661	PAS/Alcian blue	652:666	PAS/Alcian blue (for polysaccharides)	652:688	The digestive gland was stained with the following techniques: PAS/Alcian blue (for polysaccharides), bromophenol blue (for protein), von Kossa (for bound calcium) and Baker (for lipids).
23992941	4	35	theme	blue	663:666	arg1	techniques					640:649	the following techniques	626:649	the following techniques: PAS/Alcian blue (for polysaccharides), bromophenol blue (for protein), von Kossa (for bound calcium) and Baker (for lipids)	626:774	The digestive gland was stained with the following techniques: PAS/Alcian blue (for polysaccharides), bromophenol blue (for protein), von Kossa (for bound calcium) and Baker (for lipids).
23992941	3	36	theme	cell	462:465	arg1	types					467:471	Five cell types	457:471	Five cell types	457:471	Five cell types were identified in this digestive gland: B (vesicular), E (embryonic), F (fibrillar), M (basal) and R (resorptive).
23992941	1	37	theme	developed	302:310	arg1	gonads					312:317	developed gonads	302:317	developed gonads	302:317	This study provides histochemical data of the hepatopancreatic cells of adult female pink-shrimp (Farfantepenaeus brasiliensis) at two different developmental stages (those with developed gonads and those with exhausted gonads).
23992941	7	38	theme	amounts	1120:1126	arg1	presence					1102:1109	the presence	1098:1109	the presence of large amounts of proteins observed in R cells	1098:1158	The F and M cells were the most marked by the presence of large amounts of proteins observed in R cells and also inside the vacuoles of B cells.
23992941	7	39	dep	F	1060:1060	arg1	The					1056:1058	The	1056:1058	The	1056:1058	The F and M cells were the most marked by the presence of large amounts of proteins observed in R cells and also inside the vacuoles of B cells.
23992941	11	40	theme	histochemical	1443:1455	arg1	composition					1457:1467	the histochemical composition	1439:1467	the histochemical composition of these hepatopancreatic cells	1439:1499	The similarities in the histochemical composition of these hepatopancreatic cells in females with developed gonads, compared to exhausted ones, is justified by the fact that transfer of these elements to the oocytes occurs, in significant quantity, only during the initial stages of gonadal development in F. brasiliensis.
23992941	11	41	theme	significant	1646:1656	arg1	quantity					1658:1665	significant quantity	1646:1665	significant quantity	1646:1665	The similarities in the histochemical composition of these hepatopancreatic cells in females with developed gonads, compared to exhausted ones, is justified by the fact that transfer of these elements to the oocytes occurs, in significant quantity, only during the initial stages of gonadal development in F. brasiliensis.
23992941	0	42	theme	hepatopancreas	27:40	arg1	study					14:18	Histochemical study	0:18	Histochemical study of the hepatopancreas in adult females of the pink-shrimp	0:76	Histochemical study of the hepatopancreas in adult females of the pink-shrimp Farfantepenaeus brasiliensis Latreille, 1817.
23992941	5	43	located	found	803:807	arg2	glycoconjugates					782:796	Acid glycoconjugates	777:796	Acid glycoconjugates	777:796	Acid glycoconjugates were found inside vacuoles in the R cells, while neutral polysaccharides were present in the B cells and near to the microvilli.
23992941	5	43	located	found	803:807	arg1	cells					834:838	the R cells	828:838	the R cells	828:838	Acid glycoconjugates were found inside vacuoles in the R cells, while neutral polysaccharides were present in the B cells and near to the microvilli.
23992941	0	44	theme	adult	45:49	arg1	females					51:57	adult females	45:57	adult females of the pink-shrimp	45:76	Histochemical study of the hepatopancreas in adult females of the pink-shrimp Farfantepenaeus brasiliensis Latreille, 1817.
23992941	11	45	theme	hepatopancreatic	1478:1493	arg1	cells					1495:1499	these hepatopancreatic cells	1472:1499	these hepatopancreatic cells	1472:1499	The similarities in the histochemical composition of these hepatopancreatic cells in females with developed gonads, compared to exhausted ones, is justified by the fact that transfer of these elements to the oocytes occurs, in significant quantity, only during the initial stages of gonadal development in F. brasiliensis.
23992941	5	46	located	present	876:882	arg2	polysaccharides					855:869	neutral polysaccharides	847:869	neutral polysaccharides	847:869	Acid glycoconjugates were found inside vacuoles in the R cells, while neutral polysaccharides were present in the B cells and near to the microvilli.
23992941	5	46	located	present	876:882	arg1	cells					893:897	the B cells	887:897	the B cells	887:897	Acid glycoconjugates were found inside vacuoles in the R cells, while neutral polysaccharides were present in the B cells and near to the microvilli.
23992941	3	47	dep	E	529:529	arg1	embryonic					532:540	embryonic	532:540	embryonic	532:540	Five cell types were identified in this digestive gland: B (vesicular), E (embryonic), F (fibrillar), M (basal) and R (resorptive).
23992941	3	48	dep	F	544:544	arg1	fibrillar					547:555	fibrillar	547:555	fibrillar	547:555	Five cell types were identified in this digestive gland: B (vesicular), E (embryonic), F (fibrillar), M (basal) and R (resorptive).
23992941	11	49	from	F.	1725:1726	arg1	stages					1692:1697	the initial stages	1680:1697	the initial stages of gonadal development in F. brasiliensis	1680:1739	The similarities in the histochemical composition of these hepatopancreatic cells in females with developed gonads, compared to exhausted ones, is justified by the fact that transfer of these elements to the oocytes occurs, in significant quantity, only during the initial stages of gonadal development in F. brasiliensis.
23992941	2	50	theme	brasiliensis	360:371	arg1	females					373:379	The F. brasiliensis females	353:379	The F. brasiliensis females	353:379	The F. brasiliensis females were collected in seawater off the Guarapari coast, Espirito Santo, Brazil.
23992941	1	51	theme	different	259:267	arg1	stages					283:288	two different developmental stages	255:288	two different developmental stages (those with developed gonads and those with exhausted gonads)	255:350	This study provides histochemical data of the hepatopancreatic cells of adult female pink-shrimp (Farfantepenaeus brasiliensis) at two different developmental stages (those with developed gonads and those with exhausted gonads).
23992941	4	52	theme	bound	738:742	arg1	calcium					744:750	bound calcium	738:750	bound calcium	738:750	The digestive gland was stained with the following techniques: PAS/Alcian blue (for polysaccharides), bromophenol blue (for protein), von Kossa (for bound calcium) and Baker (for lipids).
23992941	6	53	with	females	930:936	arg1	gonads					953:958	exhausted gonads	943:958	exhausted gonads	943:958	In females with exhausted gonads polysaccharides were also seen in the intertubular spaces and inside the lumina of the tubules.
23992941	1	54	theme	hepatopancreatic	170:185	arg1	cells					187:191	the hepatopancreatic cells	166:191	the hepatopancreatic cells of adult female pink-shrimp	166:219	This study provides histochemical data of the hepatopancreatic cells of adult female pink-shrimp (Farfantepenaeus brasiliensis) at two different developmental stages (those with developed gonads and those with exhausted gonads).
23992941	4	55	theme	von	723:725	arg1	techniques					640:649	the following techniques	626:649	the following techniques: PAS/Alcian blue (for polysaccharides), bromophenol blue (for protein), von Kossa (for bound calcium) and Baker (for lipids)	626:774	The digestive gland was stained with the following techniques: PAS/Alcian blue (for polysaccharides), bromophenol blue (for protein), von Kossa (for bound calcium) and Baker (for lipids).
23992941	4	55	theme	von	723:725	arg1	Kossa					727:731	von Kossa	723:731	von Kossa (for bound calcium)	723:751	The digestive gland was stained with the following techniques: PAS/Alcian blue (for polysaccharides), bromophenol blue (for protein), von Kossa (for bound calcium) and Baker (for lipids).
23992941	11	56	from	development	1710:1720	arg1	F.					1725:1726	F.	1725:1726	F.	1725:1726	The similarities in the histochemical composition of these hepatopancreatic cells in females with developed gonads, compared to exhausted ones, is justified by the fact that transfer of these elements to the oocytes occurs, in significant quantity, only during the initial stages of gonadal development in F. brasiliensis.
23992941	12	57	theme	calcium	1810:1816	arg1	salts					1818:1822	calcium salts	1810:1822	calcium salts	1810:1822	Also, they may be more related to the molt stage, as in the case of calcium salts.
23992941	5	58	from	cells	893:897	arg1	present					876:882	present	876:882	present	876:882	Acid glycoconjugates were found inside vacuoles in the R cells, while neutral polysaccharides were present in the B cells and near to the microvilli.
23992941	1	59	theme	female	202:207	arg1	pink-shrimp					209:219	adult female pink-shrimp	196:219	adult female pink-shrimp	196:219	This study provides histochemical data of the hepatopancreatic cells of adult female pink-shrimp (Farfantepenaeus brasiliensis) at two different developmental stages (those with developed gonads and those with exhausted gonads).
23992941	7	60	theme	cells	1194:1198	arg1	vacuoles					1180:1187	the vacuoles	1176:1187	the vacuoles of B cells	1176:1198	The F and M cells were the most marked by the presence of large amounts of proteins observed in R cells and also inside the vacuoles of B cells.
23992941	1	61	theme	histochemical	144:156	arg1	data					158:161	histochemical data	144:161	histochemical data of the hepatopancreatic cells of adult female pink-shrimp	144:219	This study provides histochemical data of the hepatopancreatic cells of adult female pink-shrimp (Farfantepenaeus brasiliensis) at two different developmental stages (those with developed gonads and those with exhausted gonads).
23992941	11	62	theme	exhausted	1547:1555	arg1	ones					1557:1560	exhausted ones	1547:1560	exhausted ones	1547:1560	The similarities in the histochemical composition of these hepatopancreatic cells in females with developed gonads, compared to exhausted ones, is justified by the fact that transfer of these elements to the oocytes occurs, in significant quantity, only during the initial stages of gonadal development in F. brasiliensis.
23992941	11	63	theme	initial	1684:1690	arg1	stages					1692:1697	the initial stages	1680:1697	the initial stages of gonadal development in F. brasiliensis	1680:1739	The similarities in the histochemical composition of these hepatopancreatic cells in females with developed gonads, compared to exhausted ones, is justified by the fact that transfer of these elements to the oocytes occurs, in significant quantity, only during the initial stages of gonadal development in F. brasiliensis.
23992941	5	64	from	present	876:882	arg1	cells					893:897	the B cells	887:897	the B cells	887:897	Acid glycoconjugates were found inside vacuoles in the R cells, while neutral polysaccharides were present in the B cells and near to the microvilli.
23992941	3	65	dep	M	559:559	arg1	basal					562:566	basal	562:566	basal	562:566	Five cell types were identified in this digestive gland: B (vesicular), E (embryonic), F (fibrillar), M (basal) and R (resorptive).
23992941	8	66	theme	F	1243:1243	arg1	cells					1251:1255	the F and M cells	1239:1255	the F and M cells	1239:1255	The bound calcium was mainly found in the F and M cells.
23992941	11	67	dep	F.	1725:1726	arg1	brasiliensis					1728:1739	F. brasiliensis	1725:1739	F. brasiliensis	1725:1739	The similarities in the histochemical composition of these hepatopancreatic cells in females with developed gonads, compared to exhausted ones, is justified by the fact that transfer of these elements to the oocytes occurs, in significant quantity, only during the initial stages of gonadal development in F. brasiliensis.
23992941	8	68	theme	M	1249:1249	arg1	cells					1251:1255	the F and M cells	1239:1255	the F and M cells	1239:1255	The bound calcium was mainly found in the F and M cells.
23992941	12	69	theme	molt	1780:1783	arg1	stage					1785:1789	the molt stage	1776:1789	the molt stage	1776:1789	Also, they may be more related to the molt stage, as in the case of calcium salts.
23992941	7	70	located	observed	1140:1147	arg2	proteins					1131:1138	proteins	1131:1138	proteins observed in R cells	1131:1158	The F and M cells were the most marked by the presence of large amounts of proteins observed in R cells and also inside the vacuoles of B cells.
23992941	7	70	located	observed	1140:1147	arg1	cells					1154:1158	R cells	1152:1158	R cells	1152:1158	The F and M cells were the most marked by the presence of large amounts of proteins observed in R cells and also inside the vacuoles of B cells.
23992941	11	71	theme	development	1710:1720	arg1	stages					1692:1697	the initial stages	1680:1697	the initial stages of gonadal development in F. brasiliensis	1680:1739	The similarities in the histochemical composition of these hepatopancreatic cells in females with developed gonads, compared to exhausted ones, is justified by the fact that transfer of these elements to the oocytes occurs, in significant quantity, only during the initial stages of gonadal development in F. brasiliensis.
23992941	5	72	theme	Acid	777:780	arg1	glycoconjugates					782:796	Acid glycoconjugates	777:796	Acid glycoconjugates	777:796	Acid glycoconjugates were found inside vacuoles in the R cells, while neutral polysaccharides were present in the B cells and near to the microvilli.
23992941	5	73	attach	present	876:882	arg2	polysaccharides					855:869	neutral polysaccharides	847:869	neutral polysaccharides	847:869	Acid glycoconjugates were found inside vacuoles in the R cells, while neutral polysaccharides were present in the B cells and near to the microvilli.
23992941	5	73	attach	present	876:882	arg1	cells					893:897	the B cells	887:897	the B cells	887:897	Acid glycoconjugates were found inside vacuoles in the R cells, while neutral polysaccharides were present in the B cells and near to the microvilli.
23992941	7	74	theme	F	1060:1060	arg1	marked					1088:1093	marked	1088:1093	marked	1088:1093	The F and M cells were the most marked by the presence of large amounts of proteins observed in R cells and also inside the vacuoles of B cells.
23992941	7	74	theme	F	1060:1060	arg1	cells					1068:1072	The F and M cells	1056:1072	The F and M cells	1056:1072	The F and M cells were the most marked by the presence of large amounts of proteins observed in R cells and also inside the vacuoles of B cells.
23992941	9	75	theme	strong	1277:1282	arg1	staining					1293:1300	strong positive staining	1277:1300	strong positive staining for lipid	1277:1310	The F cells showed strong positive staining for lipid while the R cell only stained weakly.
23992941	0	76	from	study	14:18	arg1	females					51:57	adult females	45:57	adult females of the pink-shrimp	45:76	Histochemical study of the hepatopancreas in adult females of the pink-shrimp Farfantepenaeus brasiliensis Latreille, 1817.
23992941	1	77	dep	Farfantepenaeus	222:236	arg1	brasiliensis					238:249	Farfantepenaeus brasiliensis	222:249	Farfantepenaeus brasiliensis	222:249	This study provides histochemical data of the hepatopancreatic cells of adult female pink-shrimp (Farfantepenaeus brasiliensis) at two different developmental stages (those with developed gonads and those with exhausted gonads).
23992941	1	78	dep	provides	135:142	arg1	Farfantepenaeus					222:236	Farfantepenaeus	222:236	Farfantepenaeus	222:236	This study provides histochemical data of the hepatopancreatic cells of adult female pink-shrimp (Farfantepenaeus brasiliensis) at two different developmental stages (those with developed gonads and those with exhausted gonads).
23992941	5	79	theme	R	832:832	arg1	cells					834:838	the R cells	828:838	the R cells	828:838	Acid glycoconjugates were found inside vacuoles in the R cells, while neutral polysaccharides were present in the B cells and near to the microvilli.
23992941	2	80	dep	Brazil	449:454	arg1	off					408:410	off	408:410	off	408:410	The F. brasiliensis females were collected in seawater off the Guarapari coast, Espirito Santo, Brazil.
23992941	0	81	dep	Farfantepenaeus	78:92	arg1	study					14:18	Histochemical study	0:18	Histochemical study of the hepatopancreas in adult females of the pink-shrimp	0:76	Histochemical study of the hepatopancreas in adult females of the pink-shrimp Farfantepenaeus brasiliensis Latreille, 1817.
23992941	7	82	theme	large	1114:1118	arg1	proteins					1131:1138	proteins	1131:1138	proteins observed in R cells	1131:1158	The F and M cells were the most marked by the presence of large amounts of proteins observed in R cells and also inside the vacuoles of B cells.
23992941	7	82	theme	large	1114:1118	arg1	amounts					1120:1126	large amounts	1114:1126	large amounts of proteins observed in R cells	1114:1158	The F and M cells were the most marked by the presence of large amounts of proteins observed in R cells and also inside the vacuoles of B cells.
23992941	10	83	theme	staining	1398:1405	arg1	techniques					1407:1416	the applied staining techniques	1386:1416	the applied staining techniques	1386:1416	The E cells did not react to any of the applied staining techniques.
23992941	1	84	theme	exhausted	334:342	arg1	gonads					344:349	exhausted gonads	334:349	exhausted gonads	334:349	This study provides histochemical data of the hepatopancreatic cells of adult female pink-shrimp (Farfantepenaeus brasiliensis) at two different developmental stages (those with developed gonads and those with exhausted gonads).
23992941	8	85	theme	bound	1205:1209	arg1	calcium					1211:1217	The bound calcium	1201:1217	The bound calcium	1201:1217	The bound calcium was mainly found in the F and M cells.
23992941	5	86	theme	neutral	847:853	arg1	polysaccharides					855:869	neutral polysaccharides	847:869	neutral polysaccharides	847:869	Acid glycoconjugates were found inside vacuoles in the R cells, while neutral polysaccharides were present in the B cells and near to the microvilli.
23992941	6	87	theme	exhausted	943:951	arg1	gonads					953:958	exhausted gonads	943:958	exhausted gonads	943:958	In females with exhausted gonads polysaccharides were also seen in the intertubular spaces and inside the lumina of the tubules.
23992941	4	88	theme	blue	703:706	arg1	techniques					640:649	the following techniques	626:649	the following techniques: PAS/Alcian blue (for polysaccharides), bromophenol blue (for protein), von Kossa (for bound calcium) and Baker (for lipids)	626:774	The digestive gland was stained with the following techniques: PAS/Alcian blue (for polysaccharides), bromophenol blue (for protein), von Kossa (for bound calcium) and Baker (for lipids).
23992941	4	88	theme	blue	703:706	arg1	bromophenol					691:701	bromophenol blue	691:706	bromophenol blue (for protein)	691:720	The digestive gland was stained with the following techniques: PAS/Alcian blue (for polysaccharides), bromophenol blue (for protein), von Kossa (for bound calcium) and Baker (for lipids).
27730611	8	0	theme	material	971:978	arg1	mg					959:960	500 mg	955:960	500 mg of fresh material	955:978	The proposed method of purification can be used for large amount of material but can also be downscaled to 500 mg of fresh material.
27730611	7	1	theme	high	833:836	arg1	interest					838:845	high interest	833:845	high interest	833:845	All these characteristics and uses explain why its study as a true cell compartment is of high interest.
27730611	4	2	theme	cellular	454:461	arg1	compartment					463:473	the cellular compartment	450:473	the cellular compartment having the highest density	450:500	Due to its composition, the cell wall is the cellular compartment having the highest density and this property is used for its purification.
27730611	4	2	theme	cellular	454:461	arg1	wall					442:445	the cell wall	433:445	the cell wall	433:445	Due to its composition, the cell wall is the cellular compartment having the highest density and this property is used for its purification.
27730611	8	3	theme	material	916:923	arg1	material					916:923	material	916:923	material	916:923	The proposed method of purification can be used for large amount of material but can also be downscaled to 500 mg of fresh material.
27730611	8	3	theme	material	916:923	arg1	amount					906:911	large amount	900:911	large amount of material	900:923	The proposed method of purification can be used for large amount of material but can also be downscaled to 500 mg of fresh material.
27730611	8	4	theme	purification	871:882	arg1	method					861:866	The proposed method	848:866	The proposed method of purification	848:882	The proposed method of purification can be used for large amount of material but can also be downscaled to 500 mg of fresh material.
27730611	6	5	theme	bioenergy	732:740	arg1	production					718:727	the production	714:727	the production of bioenergy	714:740	It is largely used in the food and textile industries as well as for the production of bioenergy.
27730611	6	6	theme	textile	680:686	arg1	industries					688:697	the food and textile industries	667:697	the food and textile industries as well as for the production of bioenergy	667:740	It is largely used in the food and textile industries as well as for the production of bioenergy.
27730611	3	7	theme	specialized	283:293	arg1	cells					295:299	specialized cells	283:299	specialized cells	283:299	At the end of growth, specialized cells may synthesize a lignified secondary wall composed of polysaccharides (about 65 %) and lignin (about 35 %).
27730611	7	8	theme	true	805:808	arg1	compartment					815:825	a true cell compartment	803:825	a true cell compartment	803:825	All these characteristics and uses explain why its study as a true cell compartment is of high interest.
27730611	8	9	theme	large	900:904	arg1	material					916:923	material	916:923	material	916:923	The proposed method of purification can be used for large amount of material but can also be downscaled to 500 mg of fresh material.
27730611	8	9	theme	large	900:904	arg1	amount					906:911	large amount	900:911	large amount of material	900:923	The proposed method of purification can be used for large amount of material but can also be downscaled to 500 mg of fresh material.
27730611	6	10	theme	food	671:674	arg1	industries					688:697	the food and textile industries	667:697	the food and textile industries as well as for the production of bioenergy	667:740	It is largely used in the food and textile industries as well as for the production of bioenergy.
27730611	3	11	theme	growth	275:280	arg1	end					268:270	the end	264:270	the end of growth	264:280	At the end of growth, specialized cells may synthesize a lignified secondary wall composed of polysaccharides (about 65 %) and lignin (about 35 %).
27730611	8	12	theme	proposed	852:859	arg1	method					861:866	The proposed method	848:866	The proposed method of purification	848:882	The proposed method of purification can be used for large amount of material but can also be downscaled to 500 mg of fresh material.
27730611	6	13	used	used	659:662	arg2	It					645:646	It	645:646	It	645:646	It is largely used in the food and textile industries as well as for the production of bioenergy.
27730611	8	14	theme	fresh	965:969	arg1	material					971:978	fresh material	965:978	fresh material	965:978	The proposed method of purification can be used for large amount of material but can also be downscaled to 500 mg of fresh material.
27730611	9	15	theme	wall	1024:1027	arg1	preparation					1029:1039	the cell wall preparation	1015:1039	the cell wall preparation	1015:1039	Tools for checking the quality of the cell wall preparation, such as protein analysis and microscopy observation, are also provided.
27730611	4	16	theme	cell	437:440	arg1	compartment					463:473	the cellular compartment	450:473	the cellular compartment having the highest density	450:500	Due to its composition, the cell wall is the cellular compartment having the highest density and this property is used for its purification.
27730611	4	16	theme	cell	437:440	arg1	wall					442:445	the cell wall	433:445	the cell wall	433:445	Due to its composition, the cell wall is the cellular compartment having the highest density and this property is used for its purification.
27730611	2	17	theme	cell	169:172	arg1	wall					174:177	The plant primary cell wall	151:177	The plant primary cell wall	151:177	The plant primary cell wall is mainly composed of polysaccharides (90-95 % in mass) and of proteins (5-10 %).
27730611	9	18	theme	preparation	1029:1039	arg1	quality					1004:1010	the quality	1000:1010	the quality of the cell wall preparation	1000:1039	Tools for checking the quality of the cell wall preparation, such as protein analysis and microscopy observation, are also provided.
27730611	2	19	from	%	224:224	arg1	mass					229:232	mass	229:232	mass	229:232	The plant primary cell wall is mainly composed of polysaccharides (90-95 % in mass) and of proteins (5-10 %).
27730611	0	20	theme	Cell	17:20	arg1	Wall					22:25	the Cell Wall	13:25	the Cell Wall	13:25	Isolation of the Cell Wall.
27730611	7	21	theme	cell	810:813	arg1	compartment					815:825	a true cell compartment	803:825	a true cell compartment	803:825	All these characteristics and uses explain why its study as a true cell compartment is of high interest.
27730611	5	22	theme	plant	581:585	arg1	development					587:597	plant development	581:597	plant development	581:597	It plays critical roles during plant development and in response to environmental constraints.
27730611	2	23	theme	primary	161:167	arg1	wall					174:177	The plant primary cell wall	151:177	The plant primary cell wall	151:177	The plant primary cell wall is mainly composed of polysaccharides (90-95 % in mass) and of proteins (5-10 %).
27730611	2	24	theme	plant	155:159	arg1	wall					174:177	The plant primary cell wall	151:177	The plant primary cell wall	151:177	The plant primary cell wall is mainly composed of polysaccharides (90-95 % in mass) and of proteins (5-10 %).
27730611	0	25	theme	Wall	22:25	arg1	Isolation					0:8	Isolation	0:8	Isolation of the Cell Wall.	0:26	Isolation of the Cell Wall.
27730611	9	26	theme	protein	1050:1056	arg1	analysis					1058:1065	protein analysis	1050:1065	protein analysis	1050:1065	Tools for checking the quality of the cell wall preparation, such as protein analysis and microscopy observation, are also provided.
27730611	4	27	contain	having	475:480	arg1	compartment					463:473	the cellular compartment	450:473	the cellular compartment having the highest density	450:500	Due to its composition, the cell wall is the cellular compartment having the highest density and this property is used for its purification.
27730611	4	27	contain	having	475:480	arg2	density					494:500	the highest density	482:500	the highest density	482:500	Due to its composition, the cell wall is the cellular compartment having the highest density and this property is used for its purification.
27730611	4	27	contain	having	475:480	arg1	wall					442:445	the cell wall	433:445	the cell wall	433:445	Due to its composition, the cell wall is the cellular compartment having the highest density and this property is used for its purification.
27730611	5	28	theme	environmental	618:630	arg1	constraints					632:642	environmental constraints	618:642	environmental constraints	618:642	It plays critical roles during plant development and in response to environmental constraints.
27730611	3	29	theme	lignified	318:326	arg1	wall					338:341	a lignified secondary wall	316:341	a lignified secondary wall composed of polysaccharides (about 65 %) and lignin (about 35 %)	316:406	At the end of growth, specialized cells may synthesize a lignified secondary wall composed of polysaccharides (about 65 %) and lignin (about 35 %).
27730611	1	30	theme	cell	93:96	arg1	wall					98:101	the cell wall	89:101	the cell wall	89:101	This chapter describes a method allowing the purification of the cell wall for studying both polysaccharides and proteins.
27730611	3	31	theme	secondary	328:336	arg1	wall					338:341	a lignified secondary wall	316:341	a lignified secondary wall composed of polysaccharides (about 65 %) and lignin (about 35 %)	316:406	At the end of growth, specialized cells may synthesize a lignified secondary wall composed of polysaccharides (about 65 %) and lignin (about 35 %).
27730611	1	32	theme	wall	98:101	arg1	purification					73:84	the purification	69:84	the purification of the cell wall for studying both polysaccharides and proteins	69:148	This chapter describes a method allowing the purification of the cell wall for studying both polysaccharides and proteins.
27730611	5	33	theme	critical	559:566	arg1	roles					568:572	critical roles	559:572	critical roles	559:572	It plays critical roles during plant development and in response to environmental constraints.
27730611	9	34	theme	cell	1019:1022	arg1	preparation					1029:1039	the cell wall preparation	1015:1039	the cell wall preparation	1015:1039	Tools for checking the quality of the cell wall preparation, such as protein analysis and microscopy observation, are also provided.
27730611	4	35	theme	highest	486:492	arg1	density					494:500	the highest density	482:500	the highest density	482:500	Due to its composition, the cell wall is the cellular compartment having the highest density and this property is used for its purification.
27730611	9	36	dep	analysis	1058:1065	arg1	observation					1082:1092	observation	1082:1092	observation	1082:1092	Tools for checking the quality of the cell wall preparation, such as protein analysis and microscopy observation, are also provided.
27730611	2	37	dep	polysaccharides	201:215	arg1	%					224:224	90-95 %	218:224	90-95 % in mass	218:232	The plant primary cell wall is mainly composed of polysaccharides (90-95 % in mass) and of proteins (5-10 %).
27730611	4	38	used	used	523:526	arg2	property					511:518	this property	506:518	this property	506:518	Due to its composition, the cell wall is the cellular compartment having the highest density and this property is used for its purification.
27730611	8	39	used	used	891:894	arg2	method					861:866	The proposed method	848:866	The proposed method of purification	848:882	The proposed method of purification can be used for large amount of material but can also be downscaled to 500 mg of fresh material.
26802311	3	0	theme	strain	351:356	arg1	K4					358:359	Escherichia coli strain K4	334:359	Escherichia coli strain K4	334:359	The capsular polysaccharide from Escherichia coli strain K4 is similar to chondroitin, and its biosynthesis requires a chondroitin polymerase (KfoC).
26802311	4	1	theme	sulfate	520:526	arg1	synthesis					495:503	de novo enzymatic synthesis	477:503	de novo enzymatic synthesis of chondroitin sulfate, synthesis of chondroitin,	477:553	The essential step toward de novo enzymatic synthesis of chondroitin sulfate, synthesis of chondroitin, could be achieved by employing this enzyme.
26802311	9	2	theme	GENERAL	1262:1268	arg1	SIGNIFICANCE					1270:1281	GENERAL SIGNIFICANCE	1262:1281	GENERAL SIGNIFICANCE	1262:1281	CONCLUSION AND GENERAL SIGNIFICANCE These results assist in the development of enzymatic synthesis approaches toward chimeric glycosaminoglycan oligosaccharides and designing future strategies for directed evolution of KfoC in order to create mutants toward user-defined goals.
26802311	7	3	theme	tool	948:951	arg1	enzyme					953:958	an attractive tool enzyme	934:958	it an attractive tool enzyme for use in structurally-defined chimeric glycosaminoglycan oligosaccharide synthesis	931:1043	RESULTS KfoC has broad donor substrate specificity and acceptor promiscuity, making it an attractive tool enzyme for use in structurally-defined chimeric glycosaminoglycan oligosaccharide synthesis in vitro.
26802311	8	4	theme	chondroitin	1223:1233	arg1	polymerase					1235:1244	this chondroitin polymerase	1218:1244	this chondroitin polymerase	1218:1244	In addition, the binding of donor substrate molecules regulated the affinity of KfoC for acceptors, then influenced the glycosyl transferase reaction catalyzed by this chondroitin polymerase.
26802311	8	5	theme	molecules	1099:1107	arg1	binding					1072:1078	the binding	1068:1078	the binding of donor substrate molecules	1068:1107	In addition, the binding of donor substrate molecules regulated the affinity of KfoC for acceptors, then influenced the glycosyl transferase reaction catalyzed by this chondroitin polymerase.
26802311	5	6	theme	defined	620:626	arg1	acceptors					628:636	acceptors	628:636	acceptors	628:636	METHODS Structurally defined acceptors and donor-sugars were prepared by chemoenzymatic approaches.
26802311	4	7	dep	de	477:478	arg1	enzymatic					485:493	enzymatic	485:493	enzymatic	485:493	The essential step toward de novo enzymatic synthesis of chondroitin sulfate, synthesis of chondroitin, could be achieved by employing this enzyme.
26802311	4	7	dep	de	477:478	arg1	novo					480:483	novo	480:483	novo	480:483	The essential step toward de novo enzymatic synthesis of chondroitin sulfate, synthesis of chondroitin, could be achieved by employing this enzyme.
26802311	0	8	from	Impact	0:5	arg1	polymerization					27:40	polymerization	27:40	polymerization	27:40	Impact of donor binding on polymerization catalyzed by KfoC by regulating the affinity of enzyme for acceptor.
26802311	9	9	theme	directed	1444:1451	arg1	evolution					1453:1461	directed evolution	1444:1461	directed evolution of KfoC	1444:1469	CONCLUSION AND GENERAL SIGNIFICANCE These results assist in the development of enzymatic synthesis approaches toward chimeric glycosaminoglycan oligosaccharides and designing future strategies for directed evolution of KfoC in order to create mutants toward user-defined goals.
26802311	8	10	theme	transferase	1184:1194	arg1	reaction					1196:1203	the glycosyl transferase reaction	1171:1203	the glycosyl transferase reaction catalyzed by this chondroitin polymerase	1171:1244	In addition, the binding of donor substrate molecules regulated the affinity of KfoC for acceptors, then influenced the glycosyl transferase reaction catalyzed by this chondroitin polymerase.
26802311	7	11	theme	donor	870:874	arg1	specificity					886:896	broad donor substrate specificity	864:896	broad donor substrate specificity	864:896	RESULTS KfoC has broad donor substrate specificity and acceptor promiscuity, making it an attractive tool enzyme for use in structurally-defined chimeric glycosaminoglycan oligosaccharide synthesis in vitro.
26802311	3	12	dep	Escherichia	334:344	arg1	coli					346:349	coli	346:349	coli	346:349	The capsular polysaccharide from Escherichia coli strain K4 is similar to chondroitin, and its biosynthesis requires a chondroitin polymerase (KfoC).
26802311	8	13	theme	KfoC	1135:1138	arg1	affinity					1123:1130	the affinity	1119:1130	the affinity of KfoC for acceptors	1119:1152	In addition, the binding of donor substrate molecules regulated the affinity of KfoC for acceptors, then influenced the glycosyl transferase reaction catalyzed by this chondroitin polymerase.
26802311	0	14	theme	enzyme	90:95	arg1	affinity					78:85	the affinity	74:85	the affinity of enzyme for acceptor	74:108	Impact of donor binding on polymerization catalyzed by KfoC by regulating the affinity of enzyme for acceptor.
26802311	1	15	attach	isolated	161:168	arg1	sources					182:188	animal sources	175:188	animal sources	175:188	BACKGROUND Currently marketed chondroitin sulfate isolated from animal sources and structurally quite heterogeneous.
26802311	1	15	attach	isolated	161:168	arg2	sulfate					153:159	chondroitin sulfate	141:159	chondroitin sulfate isolated from animal sources	141:188	BACKGROUND Currently marketed chondroitin sulfate isolated from animal sources and structurally quite heterogeneous.
26802311	7	16	theme	glycosaminoglycan	1001:1017	arg1	synthesis					1035:1043	structurally-defined chimeric glycosaminoglycan oligosaccharide synthesis	971:1043	structurally-defined chimeric glycosaminoglycan oligosaccharide synthesis	971:1043	RESULTS KfoC has broad donor substrate specificity and acceptor promiscuity, making it an attractive tool enzyme for use in structurally-defined chimeric glycosaminoglycan oligosaccharide synthesis in vitro.
26802311	7	17	theme	acceptor	902:909	arg1	promiscuity					911:921	acceptor promiscuity	902:921	acceptor promiscuity	902:921	RESULTS KfoC has broad donor substrate specificity and acceptor promiscuity, making it an attractive tool enzyme for use in structurally-defined chimeric glycosaminoglycan oligosaccharide synthesis in vitro.
26802311	1	18	theme	animal	175:180	arg1	sources					182:188	animal sources	175:188	animal sources	175:188	BACKGROUND Currently marketed chondroitin sulfate isolated from animal sources and structurally quite heterogeneous.
26802311	9	19	theme	KfoC	1466:1469	arg1	evolution					1453:1461	directed evolution	1444:1461	directed evolution of KfoC	1444:1469	CONCLUSION AND GENERAL SIGNIFICANCE These results assist in the development of enzymatic synthesis approaches toward chimeric glycosaminoglycan oligosaccharides and designing future strategies for directed evolution of KfoC in order to create mutants toward user-defined goals.
26802311	7	20	theme	oligosaccharide	1019:1033	arg1	synthesis					1035:1043	structurally-defined chimeric glycosaminoglycan oligosaccharide synthesis	971:1043	structurally-defined chimeric glycosaminoglycan oligosaccharide synthesis	971:1043	RESULTS KfoC has broad donor substrate specificity and acceptor promiscuity, making it an attractive tool enzyme for use in structurally-defined chimeric glycosaminoglycan oligosaccharide synthesis in vitro.
26802311	9	21	dep	CONCLUSION	1247:1256	arg1	assist					1297:1302	assist	1297:1302	assist in the development of enzymatic synthesis approaches toward chimeric glycosaminoglycan oligosaccharides and designing future strategies for directed evolution of KfoC in order to create mutants toward user-defined goals	1297:1522	CONCLUSION AND GENERAL SIGNIFICANCE These results assist in the development of enzymatic synthesis approaches toward chimeric glycosaminoglycan oligosaccharides and designing future strategies for directed evolution of KfoC in order to create mutants toward user-defined goals.
26802311	5	22	theme	chemoenzymatic	672:685	arg1	approaches					687:696	chemoenzymatic approaches	672:696	chemoenzymatic approaches	672:696	METHODS Structurally defined acceptors and donor-sugars were prepared by chemoenzymatic approaches.
26802311	7	23	theme	structurally-defined	971:990	arg1	synthesis					1035:1043	structurally-defined chimeric glycosaminoglycan oligosaccharide synthesis	971:1043	structurally-defined chimeric glycosaminoglycan oligosaccharide synthesis	971:1043	RESULTS KfoC has broad donor substrate specificity and acceptor promiscuity, making it an attractive tool enzyme for use in structurally-defined chimeric glycosaminoglycan oligosaccharide synthesis in vitro.
26802311	8	24	theme	substrate	1089:1097	arg1	molecules					1099:1107	donor substrate molecules	1083:1107	donor substrate molecules	1083:1107	In addition, the binding of donor substrate molecules regulated the affinity of KfoC for acceptors, then influenced the glycosyl transferase reaction catalyzed by this chondroitin polymerase.
26802311	0	25	theme	binding	16:22	arg1	Impact					0:5	Impact	0:5	Impact of donor binding on polymerization	0:40	Impact of donor binding on polymerization catalyzed by KfoC by regulating the affinity of enzyme for acceptor.
26802311	7	26	theme	broad	864:868	arg1	specificity					886:896	broad donor substrate specificity	864:896	broad donor substrate specificity	864:896	RESULTS KfoC has broad donor substrate specificity and acceptor promiscuity, making it an attractive tool enzyme for use in structurally-defined chimeric glycosaminoglycan oligosaccharide synthesis in vitro.
26802311	6	27	theme	pairs	831:835	arg1	affinities					776:785	the binding affinities	764:785	the binding affinities of individual substrates and donor-acceptor pairs for KfoC	764:844	In addition, surface plasmon resonance was employed to determine the binding affinities of individual substrates and donor-acceptor pairs for KfoC.
26802311	7	28	from	use	964:966	arg1	synthesis					1035:1043	structurally-defined chimeric glycosaminoglycan oligosaccharide synthesis	971:1043	structurally-defined chimeric glycosaminoglycan oligosaccharide synthesis	971:1043	RESULTS KfoC has broad donor substrate specificity and acceptor promiscuity, making it an attractive tool enzyme for use in structurally-defined chimeric glycosaminoglycan oligosaccharide synthesis in vitro.
26802311	6	29	theme	plasmon	720:726	arg1	resonance					728:736	surface plasmon resonance	712:736	surface plasmon resonance	712:736	In addition, surface plasmon resonance was employed to determine the binding affinities of individual substrates and donor-acceptor pairs for KfoC.
26802311	0	30	theme	donor	10:14	arg1	binding					16:22	donor binding	10:22	donor binding	10:22	Impact of donor binding on polymerization catalyzed by KfoC by regulating the affinity of enzyme for acceptor.
26802311	3	31	theme	capsular	305:312	arg1	similar					364:370	similar	364:370	similar	364:370	The capsular polysaccharide from Escherichia coli strain K4 is similar to chondroitin, and its biosynthesis requires a chondroitin polymerase (KfoC).
26802311	3	31	theme	capsular	305:312	arg1	polysaccharide					314:327	The capsular polysaccharide	301:327	The capsular polysaccharide from Escherichia coli strain K4	301:359	The capsular polysaccharide from Escherichia coli strain K4 is similar to chondroitin, and its biosynthesis requires a chondroitin polymerase (KfoC).
26802311	9	32	theme	enzymatic	1326:1334	arg1	approaches					1346:1355	enzymatic synthesis approaches	1326:1355	enzymatic synthesis approaches toward chimeric glycosaminoglycan oligosaccharides and designing future strategies for directed evolution of KfoC in order to create mutants toward user-defined goals	1326:1522	CONCLUSION AND GENERAL SIGNIFICANCE These results assist in the development of enzymatic synthesis approaches toward chimeric glycosaminoglycan oligosaccharides and designing future strategies for directed evolution of KfoC in order to create mutants toward user-defined goals.
26802311	7	33	contain	has	860:862	arg1	KfoC					855:858	KfoC	855:858	KfoC	855:858	RESULTS KfoC has broad donor substrate specificity and acceptor promiscuity, making it an attractive tool enzyme for use in structurally-defined chimeric glycosaminoglycan oligosaccharide synthesis in vitro.
26802311	7	33	contain	has	860:862	arg2	promiscuity					911:921	acceptor promiscuity	902:921	acceptor promiscuity	902:921	RESULTS KfoC has broad donor substrate specificity and acceptor promiscuity, making it an attractive tool enzyme for use in structurally-defined chimeric glycosaminoglycan oligosaccharide synthesis in vitro.
26802311	7	33	contain	has	860:862	arg2	specificity					886:896	broad donor substrate specificity	864:896	broad donor substrate specificity	864:896	RESULTS KfoC has broad donor substrate specificity and acceptor promiscuity, making it an attractive tool enzyme for use in structurally-defined chimeric glycosaminoglycan oligosaccharide synthesis in vitro.
26802311	6	34	theme	surface	712:718	arg1	resonance					728:736	surface plasmon resonance	712:736	surface plasmon resonance	712:736	In addition, surface plasmon resonance was employed to determine the binding affinities of individual substrates and donor-acceptor pairs for KfoC.
26802311	9	35	theme	designing	1412:1420	arg1	strategies					1429:1438	designing future strategies	1412:1438	designing future strategies for directed evolution of KfoC	1412:1469	CONCLUSION AND GENERAL SIGNIFICANCE These results assist in the development of enzymatic synthesis approaches toward chimeric glycosaminoglycan oligosaccharides and designing future strategies for directed evolution of KfoC in order to create mutants toward user-defined goals.
26802311	9	36	theme	future	1422:1427	arg1	strategies					1429:1438	designing future strategies	1412:1438	designing future strategies for directed evolution of KfoC	1412:1469	CONCLUSION AND GENERAL SIGNIFICANCE These results assist in the development of enzymatic synthesis approaches toward chimeric glycosaminoglycan oligosaccharides and designing future strategies for directed evolution of KfoC in order to create mutants toward user-defined goals.
26802311	9	37	theme	synthesis	1336:1344	arg1	approaches					1346:1355	enzymatic synthesis approaches	1326:1355	enzymatic synthesis approaches toward chimeric glycosaminoglycan oligosaccharides and designing future strategies for directed evolution of KfoC in order to create mutants toward user-defined goals	1326:1522	CONCLUSION AND GENERAL SIGNIFICANCE These results assist in the development of enzymatic synthesis approaches toward chimeric glycosaminoglycan oligosaccharides and designing future strategies for directed evolution of KfoC in order to create mutants toward user-defined goals.
26802311	2	38	theme	defined	254:260	arg1	sulfate					274:280	structurally defined chondroitin sulfate	241:280	structurally defined chondroitin sulfate	241:280	Synthesis of structurally defined chondroitin sulfate is highly desired.
26802311	7	39	theme	substrate	876:884	arg1	specificity					886:896	broad donor substrate specificity	864:896	broad donor substrate specificity	864:896	RESULTS KfoC has broad donor substrate specificity and acceptor promiscuity, making it an attractive tool enzyme for use in structurally-defined chimeric glycosaminoglycan oligosaccharide synthesis in vitro.
26802311	9	40	theme	approaches	1346:1355	arg1	development					1311:1321	the development	1307:1321	the development of enzymatic synthesis approaches toward chimeric glycosaminoglycan oligosaccharides and designing future strategies for directed evolution of KfoC in order to create mutants toward user-defined goals	1307:1522	CONCLUSION AND GENERAL SIGNIFICANCE These results assist in the development of enzymatic synthesis approaches toward chimeric glycosaminoglycan oligosaccharides and designing future strategies for directed evolution of KfoC in order to create mutants toward user-defined goals.
26802311	8	41	theme	donor	1083:1087	arg1	molecules					1099:1107	donor substrate molecules	1083:1107	donor substrate molecules	1083:1107	In addition, the binding of donor substrate molecules regulated the affinity of KfoC for acceptors, then influenced the glycosyl transferase reaction catalyzed by this chondroitin polymerase.
26802311	6	42	theme	donor-acceptor	816:829	arg1	pairs					831:835	donor-acceptor pairs	816:835	donor-acceptor pairs	816:835	In addition, surface plasmon resonance was employed to determine the binding affinities of individual substrates and donor-acceptor pairs for KfoC.
26802311	3	43	theme	chondroitin	420:430	arg1	polymerase					432:441	a chondroitin polymerase	418:441	a chondroitin polymerase (KfoC)	418:448	The capsular polysaccharide from Escherichia coli strain K4 is similar to chondroitin, and its biosynthesis requires a chondroitin polymerase (KfoC).
26802311	3	43	theme	chondroitin	420:430	arg1	KfoC					444:447	KfoC	444:447	KfoC	444:447	The capsular polysaccharide from Escherichia coli strain K4 is similar to chondroitin, and its biosynthesis requires a chondroitin polymerase (KfoC).
26802311	4	44	theme	chondroitin	542:552	arg1	synthesis					529:537	synthesis	529:537	synthesis of chondroitin	529:552	The essential step toward de novo enzymatic synthesis of chondroitin sulfate, synthesis of chondroitin, could be achieved by employing this enzyme.
26802311	4	44	theme	chondroitin	542:552	arg1	sulfate					520:526	chondroitin sulfate	508:526	chondroitin sulfate	508:526	The essential step toward de novo enzymatic synthesis of chondroitin sulfate, synthesis of chondroitin, could be achieved by employing this enzyme.
26802311	3	45	theme	Escherichia	334:344	arg1	K4					358:359	Escherichia coli strain K4	334:359	Escherichia coli strain K4	334:359	The capsular polysaccharide from Escherichia coli strain K4 is similar to chondroitin, and its biosynthesis requires a chondroitin polymerase (KfoC).
26802311	4	46	theme	de	477:478	arg1	synthesis					495:503	de novo enzymatic synthesis	477:503	de novo enzymatic synthesis of chondroitin sulfate, synthesis of chondroitin,	477:553	The essential step toward de novo enzymatic synthesis of chondroitin sulfate, synthesis of chondroitin, could be achieved by employing this enzyme.
26802311	4	47	theme	chondroitin	508:518	arg1	synthesis					529:537	synthesis	529:537	synthesis of chondroitin	529:552	The essential step toward de novo enzymatic synthesis of chondroitin sulfate, synthesis of chondroitin, could be achieved by employing this enzyme.
26802311	4	47	theme	chondroitin	508:518	arg1	sulfate					520:526	chondroitin sulfate	508:526	chondroitin sulfate	508:526	The essential step toward de novo enzymatic synthesis of chondroitin sulfate, synthesis of chondroitin, could be achieved by employing this enzyme.
26802311	9	48	theme	user-defined	1505:1516	arg1	goals					1518:1522	user-defined goals	1505:1522	user-defined goals	1505:1522	CONCLUSION AND GENERAL SIGNIFICANCE These results assist in the development of enzymatic synthesis approaches toward chimeric glycosaminoglycan oligosaccharides and designing future strategies for directed evolution of KfoC in order to create mutants toward user-defined goals.
26802311	8	49	theme	glycosyl	1175:1182	arg1	reaction					1196:1203	the glycosyl transferase reaction	1171:1203	the glycosyl transferase reaction catalyzed by this chondroitin polymerase	1171:1244	In addition, the binding of donor substrate molecules regulated the affinity of KfoC for acceptors, then influenced the glycosyl transferase reaction catalyzed by this chondroitin polymerase.
26802311	9	50	theme	chimeric	1364:1371	arg1	oligosaccharides					1391:1406	chimeric glycosaminoglycan oligosaccharides	1364:1406	chimeric glycosaminoglycan oligosaccharides	1364:1406	CONCLUSION AND GENERAL SIGNIFICANCE These results assist in the development of enzymatic synthesis approaches toward chimeric glycosaminoglycan oligosaccharides and designing future strategies for directed evolution of KfoC in order to create mutants toward user-defined goals.
26802311	9	51	theme	glycosaminoglycan	1373:1389	arg1	oligosaccharides					1391:1406	chimeric glycosaminoglycan oligosaccharides	1364:1406	chimeric glycosaminoglycan oligosaccharides	1364:1406	CONCLUSION AND GENERAL SIGNIFICANCE These results assist in the development of enzymatic synthesis approaches toward chimeric glycosaminoglycan oligosaccharides and designing future strategies for directed evolution of KfoC in order to create mutants toward user-defined goals.
26802311	6	52	theme	substrates	801:810	arg1	affinities					776:785	the binding affinities	764:785	the binding affinities of individual substrates and donor-acceptor pairs for KfoC	764:844	In addition, surface plasmon resonance was employed to determine the binding affinities of individual substrates and donor-acceptor pairs for KfoC.
26802311	7	53	theme	chimeric	992:999	arg1	synthesis					1035:1043	structurally-defined chimeric glycosaminoglycan oligosaccharide synthesis	971:1043	structurally-defined chimeric glycosaminoglycan oligosaccharide synthesis	971:1043	RESULTS KfoC has broad donor substrate specificity and acceptor promiscuity, making it an attractive tool enzyme for use in structurally-defined chimeric glycosaminoglycan oligosaccharide synthesis in vitro.
26802311	3	54	from	K4	358:359	arg1	similar					364:370	similar	364:370	similar	364:370	The capsular polysaccharide from Escherichia coli strain K4 is similar to chondroitin, and its biosynthesis requires a chondroitin polymerase (KfoC).
26802311	3	54	from	K4	358:359	arg1	polysaccharide					314:327	The capsular polysaccharide	301:327	The capsular polysaccharide from Escherichia coli strain K4	301:359	The capsular polysaccharide from Escherichia coli strain K4 is similar to chondroitin, and its biosynthesis requires a chondroitin polymerase (KfoC).
26802311	6	55	theme	individual	790:799	arg1	substrates					801:810	individual substrates	790:810	individual substrates	790:810	In addition, surface plasmon resonance was employed to determine the binding affinities of individual substrates and donor-acceptor pairs for KfoC.
26802311	4	56	theme	essential	455:463	arg1	step					465:468	The essential step	451:468	The essential step toward de novo enzymatic synthesis of chondroitin sulfate, synthesis of chondroitin,	451:553	The essential step toward de novo enzymatic synthesis of chondroitin sulfate, synthesis of chondroitin, could be achieved by employing this enzyme.
26802311	7	57	dep	it	931:932	arg1	enzyme					953:958	an attractive tool enzyme	934:958	it an attractive tool enzyme for use in structurally-defined chimeric glycosaminoglycan oligosaccharide synthesis	931:1043	RESULTS KfoC has broad donor substrate specificity and acceptor promiscuity, making it an attractive tool enzyme for use in structurally-defined chimeric glycosaminoglycan oligosaccharide synthesis in vitro.
26802311	2	58	theme	sulfate	274:280	arg1	Synthesis					228:236	Synthesis	228:236	Synthesis of structurally defined chondroitin sulfate	228:280	Synthesis of structurally defined chondroitin sulfate is highly desired.
26802311	7	59	theme	attractive	937:946	arg1	enzyme					953:958	an attractive tool enzyme	934:958	it an attractive tool enzyme for use in structurally-defined chimeric glycosaminoglycan oligosaccharide synthesis	931:1043	RESULTS KfoC has broad donor substrate specificity and acceptor promiscuity, making it an attractive tool enzyme for use in structurally-defined chimeric glycosaminoglycan oligosaccharide synthesis in vitro.
26802311	7	60	dep	RESULTS	847:853	arg1	has					860:862	has	860:862	has	860:862	RESULTS KfoC has broad donor substrate specificity and acceptor promiscuity, making it an attractive tool enzyme for use in structurally-defined chimeric glycosaminoglycan oligosaccharide synthesis in vitro.
26802311	2	61	theme	chondroitin	262:272	arg1	sulfate					274:280	structurally defined chondroitin sulfate	241:280	structurally defined chondroitin sulfate	241:280	Synthesis of structurally defined chondroitin sulfate is highly desired.
26802311	6	62	theme	binding	768:774	arg1	affinities					776:785	the binding affinities	764:785	the binding affinities of individual substrates and donor-acceptor pairs for KfoC	764:844	In addition, surface plasmon resonance was employed to determine the binding affinities of individual substrates and donor-acceptor pairs for KfoC.
26802311	1	63	theme	chondroitin	141:151	arg1	sulfate					153:159	chondroitin sulfate	141:159	chondroitin sulfate isolated from animal sources	141:188	BACKGROUND Currently marketed chondroitin sulfate isolated from animal sources and structurally quite heterogeneous.
26802311	5	64	dep	METHODS	599:605	arg1	acceptors					628:636	acceptors	628:636	acceptors	628:636	METHODS Structurally defined acceptors and donor-sugars were prepared by chemoenzymatic approaches.
26802311	5	64	dep	METHODS	599:605	arg1	donor-sugars					642:653	donor-sugars	642:653	donor-sugars	642:653	METHODS Structurally defined acceptors and donor-sugars were prepared by chemoenzymatic approaches.
28551432	1	0	theme	further	308:314	arg1	processing					316:325	further processing	308:325	further processing	308:325	The aim of this work was to compare the two most common hydrothermal pre-treatments for wood - mild steam explosion and hot water extraction - both with the prospect of enabling extraction of hemicelluloses and facilitating further processing.
28551432	1	1	theme	hemicelluloses	276:289	arg1	extraction					262:271	extraction	262:271	extraction of hemicelluloses	262:289	The aim of this work was to compare the two most common hydrothermal pre-treatments for wood - mild steam explosion and hot water extraction - both with the prospect of enabling extraction of hemicelluloses and facilitating further processing.
28551432	0	2	theme	water	66:70	arg1	extraction					72:81	hot water extraction	62:81	hot water extraction	62:81	Hydrothermal pretreatment of wood by mild steam explosion and hot water extraction.
28551432	3	3	theme	wood	662:665	arg1	chips					667:671	the pre-treated wood chips	646:671	the pre-treated wood chips (of industrially relevant size)	646:703	In this comparative study, the emphasis was placed on local composition of the pre-treated wood chips (of industrially relevant size).
28551432	4	4	theme	wood	833:836	arg1	chips					838:842	the wood chips	829:842	the wood chips	829:842	The results show that short hot water extraction treatments lead to significant variations in the local composition within the wood chips, while steam explosion accomplishes a comparably more even removal of hemicelluloses due to the advective mass transport during the explosion step.
28551432	4	5	theme	hemicelluloses	914:927	arg1	removal					903:909	a comparably more even removal	880:909	a comparably more even removal of hemicelluloses	880:927	The results show that short hot water extraction treatments lead to significant variations in the local composition within the wood chips, while steam explosion accomplishes a comparably more even removal of hemicelluloses due to the advective mass transport during the explosion step.
28551432	3	6	theme	chips	667:671	arg1	composition					631:641	local composition	625:641	local composition of the pre-treated wood chips (of industrially relevant size)	625:703	In this comparative study, the emphasis was placed on local composition of the pre-treated wood chips (of industrially relevant size).
28551432	4	7	theme	short	728:732	arg1	extraction					744:753	short hot water extraction	728:753	short hot water extraction treatments	728:764	The results show that short hot water extraction treatments lead to significant variations in the local composition within the wood chips, while steam explosion accomplishes a comparably more even removal of hemicelluloses due to the advective mass transport during the explosion step.
28551432	4	8	theme	mass	950:953	arg1	transport					955:963	the advective mass transport	936:963	the advective mass transport during the explosion step	936:989	The results show that short hot water extraction treatments lead to significant variations in the local composition within the wood chips, while steam explosion accomplishes a comparably more even removal of hemicelluloses due to the advective mass transport during the explosion step.
28551432	3	9	theme	size	699:702	arg1	chips					667:671	the pre-treated wood chips	646:671	the pre-treated wood chips (of industrially relevant size)	646:703	In this comparative study, the emphasis was placed on local composition of the pre-treated wood chips (of industrially relevant size).
28551432	4	10	theme	water	738:742	arg1	extraction					744:753	short hot water extraction	728:753	short hot water extraction treatments	728:764	The results show that short hot water extraction treatments lead to significant variations in the local composition within the wood chips, while steam explosion accomplishes a comparably more even removal of hemicelluloses due to the advective mass transport during the explosion step.
28551432	1	11	theme	common	133:138	arg1	pre-treatments					153:166	the two most common hydrothermal pre-treatments	120:166	the two most common hydrothermal pre-treatments for wood - mild steam explosion and hot water extraction - both with the prospect of enabling extraction of hemicelluloses and facilitating further processing	120:325	The aim of this work was to compare the two most common hydrothermal pre-treatments for wood - mild steam explosion and hot water extraction - both with the prospect of enabling extraction of hemicelluloses and facilitating further processing.
28551432	4	12	theme	explosion	976:984	arg1	step					986:989	the explosion step	972:989	the explosion step	972:989	The results show that short hot water extraction treatments lead to significant variations in the local composition within the wood chips, while steam explosion accomplishes a comparably more even removal of hemicelluloses due to the advective mass transport during the explosion step.
28551432	4	13	theme	local	804:808	arg1	composition					810:820	the local composition	800:820	the local composition within the wood chips	800:842	The results show that short hot water extraction treatments lead to significant variations in the local composition within the wood chips, while steam explosion accomplishes a comparably more even removal of hemicelluloses due to the advective mass transport during the explosion step.
28551432	4	14	theme	hot	734:736	arg1	extraction					744:753	short hot water extraction	728:753	short hot water extraction treatments	728:764	The results show that short hot water extraction treatments lead to significant variations in the local composition within the wood chips, while steam explosion accomplishes a comparably more even removal of hemicelluloses due to the advective mass transport during the explosion step.
28551432	1	15	theme	hydrothermal	140:151	arg1	pre-treatments					153:166	the two most common hydrothermal pre-treatments	120:166	the two most common hydrothermal pre-treatments for wood - mild steam explosion and hot water extraction - both with the prospect of enabling extraction of hemicelluloses and facilitating further processing	120:325	The aim of this work was to compare the two most common hydrothermal pre-treatments for wood - mild steam explosion and hot water extraction - both with the prospect of enabling extraction of hemicelluloses and facilitating further processing.
28551432	3	16	theme	relevant	690:697	arg1	size					699:702	industrially relevant size	677:702	industrially relevant size	677:702	In this comparative study, the emphasis was placed on local composition of the pre-treated wood chips (of industrially relevant size).
28551432	4	17	theme	advective	940:948	arg1	transport					955:963	the advective mass transport	936:963	the advective mass transport during the explosion step	936:989	The results show that short hot water extraction treatments lead to significant variations in the local composition within the wood chips, while steam explosion accomplishes a comparably more even removal of hemicelluloses due to the advective mass transport during the explosion step.
28551432	0	18	theme	Hydrothermal	0:11	arg1	pretreatment					13:24	Hydrothermal pretreatment	0:24	Hydrothermal pretreatment of wood by mild steam explosion and hot water extraction.	0:82	Hydrothermal pretreatment of wood by mild steam explosion and hot water extraction.
28551432	2	19	theme	different	421:429	arg1	conditions					431:440	different conditions	421:440	different conditions	421:440	Although both involve autohydrolysis of the lignocellulosic tissue, they are performed under different conditions: the most prominent difference is the rapid, disintegrating, discharge employed in the steam explosion opening up the structure.
28551432	2	20	theme	explosion	535:543	arg1	opening					545:551	the steam explosion opening	525:551	the steam explosion opening up the structure	525:568	Although both involve autohydrolysis of the lignocellulosic tissue, they are performed under different conditions: the most prominent difference is the rapid, disintegrating, discharge employed in the steam explosion opening up the structure.
28551432	2	21	theme	steam	529:533	arg1	opening					545:551	the steam explosion opening	525:551	the steam explosion opening up the structure	525:568	Although both involve autohydrolysis of the lignocellulosic tissue, they are performed under different conditions: the most prominent difference is the rapid, disintegrating, discharge employed in the steam explosion opening up the structure.
28551432	0	22	theme	mild	37:40	arg1	explosion					48:56	mild steam explosion	37:56	mild steam explosion	37:56	Hydrothermal pretreatment of wood by mild steam explosion and hot water extraction.
28551432	3	23	theme	local	625:629	arg1	composition					631:641	local composition	625:641	local composition of the pre-treated wood chips (of industrially relevant size)	625:703	In this comparative study, the emphasis was placed on local composition of the pre-treated wood chips (of industrially relevant size).
28551432	1	24	theme	mild	179:182	arg1	explosion					190:198	mild steam explosion	179:198	mild steam explosion	179:198	The aim of this work was to compare the two most common hydrothermal pre-treatments for wood - mild steam explosion and hot water extraction - both with the prospect of enabling extraction of hemicelluloses and facilitating further processing.
28551432	2	25	theme	prominent	452:460	arg1	discharge					503:511	the rapid, disintegrating, discharge	476:511	discharge	503:511	Although both involve autohydrolysis of the lignocellulosic tissue, they are performed under different conditions: the most prominent difference is the rapid, disintegrating, discharge employed in the steam explosion opening up the structure.
28551432	2	25	theme	prominent	452:460	arg1	difference					462:471	the most prominent difference	443:471	the most prominent difference	443:471	Although both involve autohydrolysis of the lignocellulosic tissue, they are performed under different conditions: the most prominent difference is the rapid, disintegrating, discharge employed in the steam explosion opening up the structure.
28551432	4	26	theme	even	898:901	arg1	removal					903:909	a comparably more even removal	880:909	a comparably more even removal of hemicelluloses	880:927	The results show that short hot water extraction treatments lead to significant variations in the local composition within the wood chips, while steam explosion accomplishes a comparably more even removal of hemicelluloses due to the advective mass transport during the explosion step.
28551432	0	27	theme	wood	29:32	arg1	pretreatment					13:24	Hydrothermal pretreatment	0:24	Hydrothermal pretreatment of wood by mild steam explosion and hot water extraction.	0:82	Hydrothermal pretreatment of wood by mild steam explosion and hot water extraction.
28551432	1	28	theme	steam	184:188	arg1	explosion					190:198	mild steam explosion	179:198	mild steam explosion	179:198	The aim of this work was to compare the two most common hydrothermal pre-treatments for wood - mild steam explosion and hot water extraction - both with the prospect of enabling extraction of hemicelluloses and facilitating further processing.
28551432	0	29	theme	steam	42:46	arg1	explosion					48:56	mild steam explosion	37:56	mild steam explosion	37:56	Hydrothermal pretreatment of wood by mild steam explosion and hot water extraction.
28551432	2	30	theme	tissue	388:393	arg1	autohydrolysis					350:363	autohydrolysis	350:363	autohydrolysis of the lignocellulosic tissue	350:393	Although both involve autohydrolysis of the lignocellulosic tissue, they are performed under different conditions: the most prominent difference is the rapid, disintegrating, discharge employed in the steam explosion opening up the structure.
28551432	4	31	theme	steam	851:855	arg1	explosion					857:865	steam explosion	851:865	steam explosion	851:865	The results show that short hot water extraction treatments lead to significant variations in the local composition within the wood chips, while steam explosion accomplishes a comparably more even removal of hemicelluloses due to the advective mass transport during the explosion step.
28551432	4	32	from	variations	786:795	arg1	composition					810:820	the local composition	800:820	the local composition within the wood chips	800:842	The results show that short hot water extraction treatments lead to significant variations in the local composition within the wood chips, while steam explosion accomplishes a comparably more even removal of hemicelluloses due to the advective mass transport during the explosion step.
28551432	1	33	theme	work	100:103	arg1	aim					88:90	The aim	84:90	The aim of this work	84:103	The aim of this work was to compare the two most common hydrothermal pre-treatments for wood - mild steam explosion and hot water extraction - both with the prospect of enabling extraction of hemicelluloses and facilitating further processing.
28551432	4	34	theme	significant	774:784	arg1	variations					786:795	significant variations	774:795	significant variations in the local composition within the wood chips	774:842	The results show that short hot water extraction treatments lead to significant variations in the local composition within the wood chips, while steam explosion accomplishes a comparably more even removal of hemicelluloses due to the advective mass transport during the explosion step.
28551432	3	35	theme	pre-treated	650:660	arg1	chips					667:671	the pre-treated wood chips	646:671	the pre-treated wood chips (of industrially relevant size)	646:703	In this comparative study, the emphasis was placed on local composition of the pre-treated wood chips (of industrially relevant size).
28551432	3	36	theme	comparative	579:589	arg1	study					591:595	this comparative study	574:595	this comparative study	574:595	In this comparative study, the emphasis was placed on local composition of the pre-treated wood chips (of industrially relevant size).
28551432	2	37	dep	rapid	480:484	arg1	disintegrating					487:500	disintegrating	487:500	disintegrating	487:500	Although both involve autohydrolysis of the lignocellulosic tissue, they are performed under different conditions: the most prominent difference is the rapid, disintegrating, discharge employed in the steam explosion opening up the structure.
28551432	4	38	theme	extraction	744:753	arg1	treatments					755:764	short hot water extraction treatments	728:764	short hot water extraction treatments	728:764	The results show that short hot water extraction treatments lead to significant variations in the local composition within the wood chips, while steam explosion accomplishes a comparably more even removal of hemicelluloses due to the advective mass transport during the explosion step.
28551432	0	39	theme	hot	62:64	arg1	extraction					72:81	hot water extraction	62:81	hot water extraction	62:81	Hydrothermal pretreatment of wood by mild steam explosion and hot water extraction.
28551432	1	40	dep	wood	172:175	arg1	explosion					190:198	mild steam explosion	179:198	mild steam explosion	179:198	The aim of this work was to compare the two most common hydrothermal pre-treatments for wood - mild steam explosion and hot water extraction - both with the prospect of enabling extraction of hemicelluloses and facilitating further processing.
28551432	1	40	dep	wood	172:175	arg1	both					227:230	both	227:230	both	227:230	The aim of this work was to compare the two most common hydrothermal pre-treatments for wood - mild steam explosion and hot water extraction - both with the prospect of enabling extraction of hemicelluloses and facilitating further processing.
28551432	1	40	dep	wood	172:175	arg1	extraction					214:223	hot water extraction	204:223	hot water extraction	204:223	The aim of this work was to compare the two most common hydrothermal pre-treatments for wood - mild steam explosion and hot water extraction - both with the prospect of enabling extraction of hemicelluloses and facilitating further processing.
28551432	2	41	theme	lignocellulosic	372:386	arg1	tissue					388:393	the lignocellulosic tissue	368:393	the lignocellulosic tissue	368:393	Although both involve autohydrolysis of the lignocellulosic tissue, they are performed under different conditions: the most prominent difference is the rapid, disintegrating, discharge employed in the steam explosion opening up the structure.
28551432	1	42	theme	hot	204:206	arg1	extraction					214:223	hot water extraction	204:223	hot water extraction	204:223	The aim of this work was to compare the two most common hydrothermal pre-treatments for wood - mild steam explosion and hot water extraction - both with the prospect of enabling extraction of hemicelluloses and facilitating further processing.
28551432	2	43	theme	rapid	480:484	arg1	discharge					503:511	the rapid, disintegrating, discharge	476:511	discharge	503:511	Although both involve autohydrolysis of the lignocellulosic tissue, they are performed under different conditions: the most prominent difference is the rapid, disintegrating, discharge employed in the steam explosion opening up the structure.
28551432	2	43	theme	rapid	480:484	arg1	difference					462:471	the most prominent difference	443:471	the most prominent difference	443:471	Although both involve autohydrolysis of the lignocellulosic tissue, they are performed under different conditions: the most prominent difference is the rapid, disintegrating, discharge employed in the steam explosion opening up the structure.
28551432	1	44	theme	water	208:212	arg1	extraction					214:223	hot water extraction	204:223	hot water extraction	204:223	The aim of this work was to compare the two most common hydrothermal pre-treatments for wood - mild steam explosion and hot water extraction - both with the prospect of enabling extraction of hemicelluloses and facilitating further processing.
26275459	1	0	from	characterizations	155:171	arg1	parent					294:299	its female parent	283:299	its female parent	283:299	The present study is a comparative study on preliminary characterizations and immunostimulatory activities of water-soluble polysaccharides (WSP) from newly hybrid Amomum villosum and its female parent.
26275459	1	0	from	characterizations	155:171	arg1	villosum					270:277	Amomum villosum	263:277	Amomum villosum	263:277	The present study is a comparative study on preliminary characterizations and immunostimulatory activities of water-soluble polysaccharides (WSP) from newly hybrid Amomum villosum and its female parent.
26275459	3	1	dep	in	548:549	arg1	vivo					551:554	vivo	551:554	vivo	551:554	Then, the differences of preliminary characterizations and immunostimulatory activities in vivo for these two WSP samples were compared.
26275459	0	2	theme	villosum	67:74	arg1	hybrids					49:55	novel hybrids	43:55	novel hybrids of Amomum villosum and its female parent	43:96	A comparison study on polysaccharides from novel hybrids of Amomum villosum and its female parent.
26275459	4	3	theme	thymus	846:851	arg1	level					881:885	the level	877:885	the level of serum hemolysin	877:904	Experimental results showed that WSPH and WSPP had the same monosaccharide composition, and similar ultraviolet and infrared spectra characteristics of polysaccharides, while their immunostimulatory activities, in terms of the weights of spleen and thymus, pinocytic activity and the level of serum hemolysin, showed no significant differences between the groups treated with WSPH and WSPP, respectively.
26275459	4	3	theme	thymus	846:851	arg1	weights					824:830	the weights	820:830	the weights of spleen and thymus	820:851	Experimental results showed that WSPH and WSPP had the same monosaccharide composition, and similar ultraviolet and infrared spectra characteristics of polysaccharides, while their immunostimulatory activities, in terms of the weights of spleen and thymus, pinocytic activity and the level of serum hemolysin, showed no significant differences between the groups treated with WSPH and WSPP, respectively.
26275459	4	3	theme	thymus	846:851	arg1	activity					864:871	pinocytic activity	854:871	pinocytic activity	854:871	Experimental results showed that WSPH and WSPP had the same monosaccharide composition, and similar ultraviolet and infrared spectra characteristics of polysaccharides, while their immunostimulatory activities, in terms of the weights of spleen and thymus, pinocytic activity and the level of serum hemolysin, showed no significant differences between the groups treated with WSPH and WSPP, respectively.
26275459	1	4	from	study	134:138	arg1	characterizations					155:171	preliminary characterizations	143:171	preliminary characterizations	143:171	The present study is a comparative study on preliminary characterizations and immunostimulatory activities of water-soluble polysaccharides (WSP) from newly hybrid Amomum villosum and its female parent.
26275459	1	4	from	study	134:138	arg1	activities					195:204	immunostimulatory activities	177:204	immunostimulatory activities	177:204	The present study is a comparative study on preliminary characterizations and immunostimulatory activities of water-soluble polysaccharides (WSP) from newly hybrid Amomum villosum and its female parent.
26275459	4	5	theme	monosaccharide	657:670	arg1	composition					672:682	the same monosaccharide composition	648:682	the same monosaccharide composition	648:682	Experimental results showed that WSPH and WSPP had the same monosaccharide composition, and similar ultraviolet and infrared spectra characteristics of polysaccharides, while their immunostimulatory activities, in terms of the weights of spleen and thymus, pinocytic activity and the level of serum hemolysin, showed no significant differences between the groups treated with WSPH and WSPP, respectively.
26275459	0	6	theme	female	84:89	arg1	parent					91:96	its female parent	80:96	its female parent	80:96	A comparison study on polysaccharides from novel hybrids of Amomum villosum and its female parent.
26275459	5	7	theme	novel	1105:1109	arg1	alternative					1141:1151	an acceptable alternative	1127:1151	an acceptable alternative for cultivation of A. villosum	1127:1182	Combined with the findings from other studies in our research group, these results suggested that this novel hybrid could be an acceptable alternative for cultivation of A. villosum.
26275459	5	7	theme	novel	1105:1109	arg1	hybrid					1111:1116	this novel hybrid	1100:1116	this novel hybrid	1100:1116	Combined with the findings from other studies in our research group, these results suggested that this novel hybrid could be an acceptable alternative for cultivation of A. villosum.
26275459	3	8	theme	characterizations	497:513	arg1	differences					470:480	the differences	466:480	the differences of preliminary characterizations and immunostimulatory activities in vivo for these two WSP samples	466:580	Then, the differences of preliminary characterizations and immunostimulatory activities in vivo for these two WSP samples were compared.
26275459	4	9	theme	spectra	722:728	arg1	characteristics					730:744	similar ultraviolet and infrared spectra characteristics	689:744	similar ultraviolet and infrared spectra characteristics	689:744	Experimental results showed that WSPH and WSPP had the same monosaccharide composition, and similar ultraviolet and infrared spectra characteristics of polysaccharides, while their immunostimulatory activities, in terms of the weights of spleen and thymus, pinocytic activity and the level of serum hemolysin, showed no significant differences between the groups treated with WSPH and WSPP, respectively.
26275459	5	10	theme	villosum	1175:1182	arg1	cultivation					1157:1167	cultivation	1157:1167	cultivation of A. villosum	1157:1182	Combined with the findings from other studies in our research group, these results suggested that this novel hybrid could be an acceptable alternative for cultivation of A. villosum.
26275459	4	11	theme	infrared	713:720	arg1	spectra					722:728	infrared spectra	713:728	infrared spectra	713:728	Experimental results showed that WSPH and WSPP had the same monosaccharide composition, and similar ultraviolet and infrared spectra characteristics of polysaccharides, while their immunostimulatory activities, in terms of the weights of spleen and thymus, pinocytic activity and the level of serum hemolysin, showed no significant differences between the groups treated with WSPH and WSPP, respectively.
26275459	0	12	from	hybrids	49:55	arg1	polysaccharides					22:36	polysaccharides	22:36	polysaccharides from novel hybrids of Amomum villosum and its female parent	22:96	A comparison study on polysaccharides from novel hybrids of Amomum villosum and its female parent.
26275459	4	13	theme	pinocytic	854:862	arg1	weights					824:830	the weights	820:830	the weights of spleen and thymus	820:851	Experimental results showed that WSPH and WSPP had the same monosaccharide composition, and similar ultraviolet and infrared spectra characteristics of polysaccharides, while their immunostimulatory activities, in terms of the weights of spleen and thymus, pinocytic activity and the level of serum hemolysin, showed no significant differences between the groups treated with WSPH and WSPP, respectively.
26275459	4	13	theme	pinocytic	854:862	arg1	activity					864:871	pinocytic activity	854:871	pinocytic activity	854:871	Experimental results showed that WSPH and WSPP had the same monosaccharide composition, and similar ultraviolet and infrared spectra characteristics of polysaccharides, while their immunostimulatory activities, in terms of the weights of spleen and thymus, pinocytic activity and the level of serum hemolysin, showed no significant differences between the groups treated with WSPH and WSPP, respectively.
26275459	5	14	from	studies	1040:1046	arg1	group					1064:1068	our research group	1051:1068	our research group	1051:1068	Combined with the findings from other studies in our research group, these results suggested that this novel hybrid could be an acceptable alternative for cultivation of A. villosum.
26275459	5	14	from	studies	1040:1046	arg1	findings					1020:1027	the findings	1016:1027	the findings from other studies in our research group	1016:1068	Combined with the findings from other studies in our research group, these results suggested that this novel hybrid could be an acceptable alternative for cultivation of A. villosum.
26275459	4	15	theme	Experimental	597:608	arg1	results					610:616	Experimental results	597:616	Experimental results	597:616	Experimental results showed that WSPH and WSPP had the same monosaccharide composition, and similar ultraviolet and infrared spectra characteristics of polysaccharides, while their immunostimulatory activities, in terms of the weights of spleen and thymus, pinocytic activity and the level of serum hemolysin, showed no significant differences between the groups treated with WSPH and WSPP, respectively.
26275459	1	16	theme	preliminary	143:153	arg1	characterizations					155:171	preliminary characterizations	143:171	preliminary characterizations	143:171	The present study is a comparative study on preliminary characterizations and immunostimulatory activities of water-soluble polysaccharides (WSP) from newly hybrid Amomum villosum and its female parent.
26275459	0	17	theme	parent	91:96	arg1	hybrids					49:55	novel hybrids	43:55	novel hybrids of Amomum villosum and its female parent	43:96	A comparison study on polysaccharides from novel hybrids of Amomum villosum and its female parent.
26275459	1	18	from	parent	294:299	arg1	WSP					240:242	WSP	240:242	WSP	240:242	The present study is a comparative study on preliminary characterizations and immunostimulatory activities of water-soluble polysaccharides (WSP) from newly hybrid Amomum villosum and its female parent.
26275459	1	18	from	parent	294:299	arg1	characterizations					155:171	preliminary characterizations	143:171	preliminary characterizations	143:171	The present study is a comparative study on preliminary characterizations and immunostimulatory activities of water-soluble polysaccharides (WSP) from newly hybrid Amomum villosum and its female parent.
26275459	1	18	from	parent	294:299	arg1	activities					195:204	immunostimulatory activities	177:204	immunostimulatory activities	177:204	The present study is a comparative study on preliminary characterizations and immunostimulatory activities of water-soluble polysaccharides (WSP) from newly hybrid Amomum villosum and its female parent.
26275459	1	18	from	parent	294:299	arg1	polysaccharides					223:237	water-soluble polysaccharides	209:237	water-soluble polysaccharides (WSP) from newly hybrid Amomum villosum and its female parent	209:299	The present study is a comparative study on preliminary characterizations and immunostimulatory activities of water-soluble polysaccharides (WSP) from newly hybrid Amomum villosum and its female parent.
26275459	3	19	theme	preliminary	485:495	arg1	characterizations					497:513	preliminary characterizations	485:513	preliminary characterizations	485:513	Then, the differences of preliminary characterizations and immunostimulatory activities in vivo for these two WSP samples were compared.
26275459	3	20	theme	in	548:549	arg1	characterizations					497:513	preliminary characterizations	485:513	preliminary characterizations	485:513	Then, the differences of preliminary characterizations and immunostimulatory activities in vivo for these two WSP samples were compared.
26275459	5	21	theme	acceptable	1130:1139	arg1	alternative					1141:1151	an acceptable alternative	1127:1151	an acceptable alternative for cultivation of A. villosum	1127:1182	Combined with the findings from other studies in our research group, these results suggested that this novel hybrid could be an acceptable alternative for cultivation of A. villosum.
26275459	5	21	theme	acceptable	1130:1139	arg1	hybrid					1111:1116	this novel hybrid	1100:1116	this novel hybrid	1100:1116	Combined with the findings from other studies in our research group, these results suggested that this novel hybrid could be an acceptable alternative for cultivation of A. villosum.
26275459	4	22	theme	spleen	835:840	arg1	level					881:885	the level	877:885	the level of serum hemolysin	877:904	Experimental results showed that WSPH and WSPP had the same monosaccharide composition, and similar ultraviolet and infrared spectra characteristics of polysaccharides, while their immunostimulatory activities, in terms of the weights of spleen and thymus, pinocytic activity and the level of serum hemolysin, showed no significant differences between the groups treated with WSPH and WSPP, respectively.
26275459	4	22	theme	spleen	835:840	arg1	weights					824:830	the weights	820:830	the weights of spleen and thymus	820:851	Experimental results showed that WSPH and WSPP had the same monosaccharide composition, and similar ultraviolet and infrared spectra characteristics of polysaccharides, while their immunostimulatory activities, in terms of the weights of spleen and thymus, pinocytic activity and the level of serum hemolysin, showed no significant differences between the groups treated with WSPH and WSPP, respectively.
26275459	4	22	theme	spleen	835:840	arg1	activity					864:871	pinocytic activity	854:871	pinocytic activity	854:871	Experimental results showed that WSPH and WSPP had the same monosaccharide composition, and similar ultraviolet and infrared spectra characteristics of polysaccharides, while their immunostimulatory activities, in terms of the weights of spleen and thymus, pinocytic activity and the level of serum hemolysin, showed no significant differences between the groups treated with WSPH and WSPP, respectively.
26275459	1	23	theme	immunostimulatory	177:193	arg1	activities					195:204	immunostimulatory activities	177:204	immunostimulatory activities	177:204	The present study is a comparative study on preliminary characterizations and immunostimulatory activities of water-soluble polysaccharides (WSP) from newly hybrid Amomum villosum and its female parent.
26275459	4	24	theme	hemolysin	896:904	arg1	level					881:885	the level	877:885	the level of serum hemolysin	877:904	Experimental results showed that WSPH and WSPP had the same monosaccharide composition, and similar ultraviolet and infrared spectra characteristics of polysaccharides, while their immunostimulatory activities, in terms of the weights of spleen and thymus, pinocytic activity and the level of serum hemolysin, showed no significant differences between the groups treated with WSPH and WSPP, respectively.
26275459	4	24	theme	hemolysin	896:904	arg1	weights					824:830	the weights	820:830	the weights of spleen and thymus	820:851	Experimental results showed that WSPH and WSPP had the same monosaccharide composition, and similar ultraviolet and infrared spectra characteristics of polysaccharides, while their immunostimulatory activities, in terms of the weights of spleen and thymus, pinocytic activity and the level of serum hemolysin, showed no significant differences between the groups treated with WSPH and WSPP, respectively.
26275459	4	24	theme	hemolysin	896:904	arg1	activity					864:871	pinocytic activity	854:871	pinocytic activity	854:871	Experimental results showed that WSPH and WSPP had the same monosaccharide composition, and similar ultraviolet and infrared spectra characteristics of polysaccharides, while their immunostimulatory activities, in terms of the weights of spleen and thymus, pinocytic activity and the level of serum hemolysin, showed no significant differences between the groups treated with WSPH and WSPP, respectively.
26275459	3	25	theme	immunostimulatory	519:535	arg1	activities					537:546	immunostimulatory activities	519:546	immunostimulatory activities	519:546	Then, the differences of preliminary characterizations and immunostimulatory activities in vivo for these two WSP samples were compared.
26275459	1	26	theme	female	287:292	arg1	parent					294:299	its female parent	283:299	its female parent	283:299	The present study is a comparative study on preliminary characterizations and immunostimulatory activities of water-soluble polysaccharides (WSP) from newly hybrid Amomum villosum and its female parent.
26275459	1	27	from	villosum	270:277	arg1	WSP					240:242	WSP	240:242	WSP	240:242	The present study is a comparative study on preliminary characterizations and immunostimulatory activities of water-soluble polysaccharides (WSP) from newly hybrid Amomum villosum and its female parent.
26275459	1	27	from	villosum	270:277	arg1	characterizations					155:171	preliminary characterizations	143:171	preliminary characterizations	143:171	The present study is a comparative study on preliminary characterizations and immunostimulatory activities of water-soluble polysaccharides (WSP) from newly hybrid Amomum villosum and its female parent.
26275459	1	27	from	villosum	270:277	arg1	activities					195:204	immunostimulatory activities	177:204	immunostimulatory activities	177:204	The present study is a comparative study on preliminary characterizations and immunostimulatory activities of water-soluble polysaccharides (WSP) from newly hybrid Amomum villosum and its female parent.
26275459	1	27	from	villosum	270:277	arg1	polysaccharides					223:237	water-soluble polysaccharides	209:237	water-soluble polysaccharides (WSP) from newly hybrid Amomum villosum and its female parent	209:299	The present study is a comparative study on preliminary characterizations and immunostimulatory activities of water-soluble polysaccharides (WSP) from newly hybrid Amomum villosum and its female parent.
26275459	3	28	theme	activities	537:546	arg1	differences					470:480	the differences	466:480	the differences of preliminary characterizations and immunostimulatory activities in vivo for these two WSP samples	466:580	Then, the differences of preliminary characterizations and immunostimulatory activities in vivo for these two WSP samples were compared.
26275459	2	29	theme	Spring	396:401	arg1	WSPH					409:412	WSPH	409:412	WSPH	409:412	First, two kinds of WSP were extracted, respectively from the newly hybrid A. villosum called Spring No.1 (WSPH) and its female parent-Longfruit No.2 (WSPP).
26275459	2	29	theme	Spring	396:401	arg1	No.1					403:406	the newly hybrid A. villosum called Spring No.1	360:406	the newly hybrid A. villosum called Spring No.1 (WSPH)	360:413	First, two kinds of WSP were extracted, respectively from the newly hybrid A. villosum called Spring No.1 (WSPH) and its female parent-Longfruit No.2 (WSPP).
26275459	2	30	theme	WSP	322:324	arg1	kinds					313:317	two kinds	309:317	two kinds of WSP	309:324	First, two kinds of WSP were extracted, respectively from the newly hybrid A. villosum called Spring No.1 (WSPH) and its female parent-Longfruit No.2 (WSPP).
26275459	1	31	theme	water-soluble	209:221	arg1	WSP					240:242	WSP	240:242	WSP	240:242	The present study is a comparative study on preliminary characterizations and immunostimulatory activities of water-soluble polysaccharides (WSP) from newly hybrid Amomum villosum and its female parent.
26275459	1	31	theme	water-soluble	209:221	arg1	polysaccharides					223:237	water-soluble polysaccharides	209:237	water-soluble polysaccharides (WSP) from newly hybrid Amomum villosum and its female parent	209:299	The present study is a comparative study on preliminary characterizations and immunostimulatory activities of water-soluble polysaccharides (WSP) from newly hybrid Amomum villosum and its female parent.
26275459	2	32	dep	called	389:394	arg1	hybrid					370:375	hybrid	370:375	hybrid	370:375	First, two kinds of WSP were extracted, respectively from the newly hybrid A. villosum called Spring No.1 (WSPH) and its female parent-Longfruit No.2 (WSPP).
26275459	2	32	dep	called	389:394	arg1	villosum					380:387	villosum	380:387	villosum	380:387	First, two kinds of WSP were extracted, respectively from the newly hybrid A. villosum called Spring No.1 (WSPH) and its female parent-Longfruit No.2 (WSPP).
26275459	2	33	theme	called	389:394	arg1	WSPH					409:412	WSPH	409:412	WSPH	409:412	First, two kinds of WSP were extracted, respectively from the newly hybrid A. villosum called Spring No.1 (WSPH) and its female parent-Longfruit No.2 (WSPP).
26275459	2	33	theme	called	389:394	arg1	No.1					403:406	the newly hybrid A. villosum called Spring No.1	360:406	the newly hybrid A. villosum called Spring No.1 (WSPH)	360:413	First, two kinds of WSP were extracted, respectively from the newly hybrid A. villosum called Spring No.1 (WSPH) and its female parent-Longfruit No.2 (WSPP).
26275459	2	34	theme	parent-Longfruit	430:445	arg1	WSPP					453:456	WSPP	453:456	WSPP	453:456	First, two kinds of WSP were extracted, respectively from the newly hybrid A. villosum called Spring No.1 (WSPH) and its female parent-Longfruit No.2 (WSPP).
26275459	2	34	theme	parent-Longfruit	430:445	arg1	No.2					447:450	its female parent-Longfruit No.2	419:450	its female parent-Longfruit No.2 (WSPP)	419:457	First, two kinds of WSP were extracted, respectively from the newly hybrid A. villosum called Spring No.1 (WSPH) and its female parent-Longfruit No.2 (WSPP).
26275459	5	35	with	Combined	1002:1009	arg1	findings					1020:1027	the findings	1016:1027	the findings from other studies in our research group	1016:1068	Combined with the findings from other studies in our research group, these results suggested that this novel hybrid could be an acceptable alternative for cultivation of A. villosum.
26275459	3	36	theme	WSP	570:572	arg1	samples					574:580	these two WSP samples	560:580	these two WSP samples	560:580	Then, the differences of preliminary characterizations and immunostimulatory activities in vivo for these two WSP samples were compared.
26275459	1	37	theme	polysaccharides	223:237	arg1	characterizations					155:171	preliminary characterizations	143:171	preliminary characterizations	143:171	The present study is a comparative study on preliminary characterizations and immunostimulatory activities of water-soluble polysaccharides (WSP) from newly hybrid Amomum villosum and its female parent.
26275459	1	37	theme	polysaccharides	223:237	arg1	activities					195:204	immunostimulatory activities	177:204	immunostimulatory activities	177:204	The present study is a comparative study on preliminary characterizations and immunostimulatory activities of water-soluble polysaccharides (WSP) from newly hybrid Amomum villosum and its female parent.
26275459	2	38	theme	female	423:428	arg1	WSPP					453:456	WSPP	453:456	WSPP	453:456	First, two kinds of WSP were extracted, respectively from the newly hybrid A. villosum called Spring No.1 (WSPH) and its female parent-Longfruit No.2 (WSPP).
26275459	2	38	theme	female	423:428	arg1	No.2					447:450	its female parent-Longfruit No.2	419:450	its female parent-Longfruit No.2 (WSPP)	419:457	First, two kinds of WSP were extracted, respectively from the newly hybrid A. villosum called Spring No.1 (WSPH) and its female parent-Longfruit No.2 (WSPP).
26275459	4	39	theme	significant	917:927	arg1	differences					929:939	no significant differences	914:939	no significant differences between the groups treated with WSPH and WSPP	914:985	Experimental results showed that WSPH and WSPP had the same monosaccharide composition, and similar ultraviolet and infrared spectra characteristics of polysaccharides, while their immunostimulatory activities, in terms of the weights of spleen and thymus, pinocytic activity and the level of serum hemolysin, showed no significant differences between the groups treated with WSPH and WSPP, respectively.
26275459	5	40	theme	research	1055:1062	arg1	group					1064:1068	our research group	1051:1068	our research group	1051:1068	Combined with the findings from other studies in our research group, these results suggested that this novel hybrid could be an acceptable alternative for cultivation of A. villosum.
26275459	0	41	theme	novel	43:47	arg1	hybrids					49:55	novel hybrids	43:55	novel hybrids of Amomum villosum and its female parent	43:96	A comparison study on polysaccharides from novel hybrids of Amomum villosum and its female parent.
26275459	4	42	theme	weights	824:830	arg1	terms					811:815	terms	811:815	terms of the weights of spleen and thymus, pinocytic activity and the level of serum hemolysin	811:904	Experimental results showed that WSPH and WSPP had the same monosaccharide composition, and similar ultraviolet and infrared spectra characteristics of polysaccharides, while their immunostimulatory activities, in terms of the weights of spleen and thymus, pinocytic activity and the level of serum hemolysin, showed no significant differences between the groups treated with WSPH and WSPP, respectively.
26275459	1	43	from	activities	195:204	arg1	parent					294:299	its female parent	283:299	its female parent	283:299	The present study is a comparative study on preliminary characterizations and immunostimulatory activities of water-soluble polysaccharides (WSP) from newly hybrid Amomum villosum and its female parent.
26275459	1	43	from	activities	195:204	arg1	villosum					270:277	Amomum villosum	263:277	Amomum villosum	263:277	The present study is a comparative study on preliminary characterizations and immunostimulatory activities of water-soluble polysaccharides (WSP) from newly hybrid Amomum villosum and its female parent.
26275459	1	44	theme	present	103:109	arg1	study					111:115	The present study	99:115	The present study	99:115	The present study is a comparative study on preliminary characterizations and immunostimulatory activities of water-soluble polysaccharides (WSP) from newly hybrid Amomum villosum and its female parent.
26275459	1	44	theme	present	103:109	arg1	study					134:138	a comparative study	120:138	a comparative study on preliminary characterizations and immunostimulatory activities of water-soluble polysaccharides (WSP) from newly hybrid Amomum villosum and its female parent	120:299	The present study is a comparative study on preliminary characterizations and immunostimulatory activities of water-soluble polysaccharides (WSP) from newly hybrid Amomum villosum and its female parent.
26275459	4	45	contain	had	644:646	arg2	composition					672:682	the same monosaccharide composition	648:682	the same monosaccharide composition	648:682	Experimental results showed that WSPH and WSPP had the same monosaccharide composition, and similar ultraviolet and infrared spectra characteristics of polysaccharides, while their immunostimulatory activities, in terms of the weights of spleen and thymus, pinocytic activity and the level of serum hemolysin, showed no significant differences between the groups treated with WSPH and WSPP, respectively.
26275459	4	45	contain	had	644:646	arg1	WSPP					639:642	WSPP	639:642	WSPP	639:642	Experimental results showed that WSPH and WSPP had the same monosaccharide composition, and similar ultraviolet and infrared spectra characteristics of polysaccharides, while their immunostimulatory activities, in terms of the weights of spleen and thymus, pinocytic activity and the level of serum hemolysin, showed no significant differences between the groups treated with WSPH and WSPP, respectively.
26275459	4	45	contain	had	644:646	arg2	characteristics					730:744	similar ultraviolet and infrared spectra characteristics	689:744	similar ultraviolet and infrared spectra characteristics	689:744	Experimental results showed that WSPH and WSPP had the same monosaccharide composition, and similar ultraviolet and infrared spectra characteristics of polysaccharides, while their immunostimulatory activities, in terms of the weights of spleen and thymus, pinocytic activity and the level of serum hemolysin, showed no significant differences between the groups treated with WSPH and WSPP, respectively.
26275459	4	45	contain	had	644:646	arg1	WSPH					630:633	WSPH	630:633	WSPH	630:633	Experimental results showed that WSPH and WSPP had the same monosaccharide composition, and similar ultraviolet and infrared spectra characteristics of polysaccharides, while their immunostimulatory activities, in terms of the weights of spleen and thymus, pinocytic activity and the level of serum hemolysin, showed no significant differences between the groups treated with WSPH and WSPP, respectively.
26275459	4	46	theme	immunostimulatory	778:794	arg1	activities					796:805	their immunostimulatory activities	772:805	their immunostimulatory activities	772:805	Experimental results showed that WSPH and WSPP had the same monosaccharide composition, and similar ultraviolet and infrared spectra characteristics of polysaccharides, while their immunostimulatory activities, in terms of the weights of spleen and thymus, pinocytic activity and the level of serum hemolysin, showed no significant differences between the groups treated with WSPH and WSPP, respectively.
26275459	4	47	theme	ultraviolet	697:707	arg1	characteristics					730:744	similar ultraviolet and infrared spectra characteristics	689:744	similar ultraviolet and infrared spectra characteristics	689:744	Experimental results showed that WSPH and WSPP had the same monosaccharide composition, and similar ultraviolet and infrared spectra characteristics of polysaccharides, while their immunostimulatory activities, in terms of the weights of spleen and thymus, pinocytic activity and the level of serum hemolysin, showed no significant differences between the groups treated with WSPH and WSPP, respectively.
26275459	4	48	theme	polysaccharides	749:763	arg1	composition					672:682	the same monosaccharide composition	648:682	the same monosaccharide composition	648:682	Experimental results showed that WSPH and WSPP had the same monosaccharide composition, and similar ultraviolet and infrared spectra characteristics of polysaccharides, while their immunostimulatory activities, in terms of the weights of spleen and thymus, pinocytic activity and the level of serum hemolysin, showed no significant differences between the groups treated with WSPH and WSPP, respectively.
26275459	4	48	theme	polysaccharides	749:763	arg1	characteristics					730:744	similar ultraviolet and infrared spectra characteristics	689:744	similar ultraviolet and infrared spectra characteristics	689:744	Experimental results showed that WSPH and WSPP had the same monosaccharide composition, and similar ultraviolet and infrared spectra characteristics of polysaccharides, while their immunostimulatory activities, in terms of the weights of spleen and thymus, pinocytic activity and the level of serum hemolysin, showed no significant differences between the groups treated with WSPH and WSPP, respectively.
26275459	4	49	theme	similar	689:695	arg1	ultraviolet					697:707	similar ultraviolet	689:707	similar ultraviolet	689:707	Experimental results showed that WSPH and WSPP had the same monosaccharide composition, and similar ultraviolet and infrared spectra characteristics of polysaccharides, while their immunostimulatory activities, in terms of the weights of spleen and thymus, pinocytic activity and the level of serum hemolysin, showed no significant differences between the groups treated with WSPH and WSPP, respectively.
26275459	4	50	theme	serum	890:894	arg1	hemolysin					896:904	serum hemolysin	890:904	serum hemolysin	890:904	Experimental results showed that WSPH and WSPP had the same monosaccharide composition, and similar ultraviolet and infrared spectra characteristics of polysaccharides, while their immunostimulatory activities, in terms of the weights of spleen and thymus, pinocytic activity and the level of serum hemolysin, showed no significant differences between the groups treated with WSPH and WSPP, respectively.
26275459	5	51	theme	other	1034:1038	arg1	studies					1040:1046	other studies	1034:1046	other studies in our research group	1034:1068	Combined with the findings from other studies in our research group, these results suggested that this novel hybrid could be an acceptable alternative for cultivation of A. villosum.
26275459	4	52	from	activities	796:805	arg1	terms					811:815	terms	811:815	terms of the weights of spleen and thymus, pinocytic activity and the level of serum hemolysin	811:904	Experimental results showed that WSPH and WSPP had the same monosaccharide composition, and similar ultraviolet and infrared spectra characteristics of polysaccharides, while their immunostimulatory activities, in terms of the weights of spleen and thymus, pinocytic activity and the level of serum hemolysin, showed no significant differences between the groups treated with WSPH and WSPP, respectively.
26275459	4	53	theme	same	652:655	arg1	composition					672:682	the same monosaccharide composition	648:682	the same monosaccharide composition	648:682	Experimental results showed that WSPH and WSPP had the same monosaccharide composition, and similar ultraviolet and infrared spectra characteristics of polysaccharides, while their immunostimulatory activities, in terms of the weights of spleen and thymus, pinocytic activity and the level of serum hemolysin, showed no significant differences between the groups treated with WSPH and WSPP, respectively.
26275459	1	54	theme	comparative	122:132	arg1	study					111:115	The present study	99:115	The present study	99:115	The present study is a comparative study on preliminary characterizations and immunostimulatory activities of water-soluble polysaccharides (WSP) from newly hybrid Amomum villosum and its female parent.
26275459	1	54	theme	comparative	122:132	arg1	study					134:138	a comparative study	120:138	a comparative study on preliminary characterizations and immunostimulatory activities of water-soluble polysaccharides (WSP) from newly hybrid Amomum villosum and its female parent	120:299	The present study is a comparative study on preliminary characterizations and immunostimulatory activities of water-soluble polysaccharides (WSP) from newly hybrid Amomum villosum and its female parent.
25756836	6	0	theme	cellulose-based	1227:1241	arg1	walls					1248:1252	cellulose-based cell walls	1227:1252	cellulose-based cell walls	1227:1252	Association of cell-wall polymers with cellulose driven by entropic amelioration of high energy cellulose/water interfaces should be considered as a third type of interaction within cellulose-based cell walls, in addition to molecular binding (enthalpic driving force) exhibited by, for example, xyloglucans or mannans, and interpenetrating networks based on, for example, pectins.
25756836	6	1	theme	molecular	1270:1278	arg1	binding					1280:1286	molecular binding	1270:1286	molecular binding (enthalpic driving force) exhibited by, for example, xyloglucans or mannans, and interpenetrating networks based on, for example, pectins	1270:1424	Association of cell-wall polymers with cellulose driven by entropic amelioration of high energy cellulose/water interfaces should be considered as a third type of interaction within cellulose-based cell walls, in addition to molecular binding (enthalpic driving force) exhibited by, for example, xyloglucans or mannans, and interpenetrating networks based on, for example, pectins.
25756836	6	1	theme	molecular	1270:1278	arg1	force					1307:1311	enthalpic driving force	1289:1311	enthalpic driving force	1289:1311	Association of cell-wall polymers with cellulose driven by entropic amelioration of high energy cellulose/water interfaces should be considered as a third type of interaction within cellulose-based cell walls, in addition to molecular binding (enthalpic driving force) exhibited by, for example, xyloglucans or mannans, and interpenetrating networks based on, for example, pectins.
25756836	4	2	theme	In	676:677	arg1	depletion					684:692	In situ depletion	676:692	In situ depletion of arabinoxylan arabinose side chains within preformed cellulose composites	676:768	In situ depletion of arabinoxylan arabinose side chains within preformed cellulose composites resulted in phase separation, with only limited enhancement of xylan-cellulose interactions.
25756836	6	3	theme	energy	1134:1139	arg1	interfaces					1157:1166	high energy cellulose/water interfaces	1129:1166	high energy cellulose/water interfaces	1129:1166	Association of cell-wall polymers with cellulose driven by entropic amelioration of high energy cellulose/water interfaces should be considered as a third type of interaction within cellulose-based cell walls, in addition to molecular binding (enthalpic driving force) exhibited by, for example, xyloglucans or mannans, and interpenetrating networks based on, for example, pectins.
25756836	0	4	theme	-β-glucan	43:51	arg1	Interactions					0:11	Interactions	0:11	Interactions of arabinoxylan and (1,3)(1,4)-β-glucan with cellulose networks.	0:76	Interactions of arabinoxylan and (1,3)(1,4)-β-glucan with cellulose networks.
25756836	1	5	theme	cereal/grass	262:273	arg1	walls					288:292	cereal/grass primary cell walls	262:292	cereal/grass primary cell walls	262:292	To identify interactions of relevance to the structure and properties of the primary cell walls of cereals and grasses, we used arabinoxylan and (1,3)(1,4)-β-glucan, major polymers in cereal/grass primary cell walls, to construct composites with cellulose produced by Gluconacetobacter xylinus.
25756836	3	6	theme	cell	637:640	arg1	walls					642:646	nongrass primary cell walls	620:646	nongrass primary cell walls	620:646	Mechanical properties were modestly affected compared with xyloglucan or pectin (characteristic components of nongrass primary cell walls) composites with cellulose.
25756836	5	7	theme	cellulose	1025:1033	arg1	networks					1035:1042	cellulose networks	1025:1042	cellulose networks	1025:1042	These results suggest that arabinoxylan and (1 → 3)(1 → 4)-β-d-glucan are not functional homologues for either xyloglucan or pectin in the way they interact with cellulose networks.
25756836	6	8	theme	cell-wall	1060:1068	arg1	polymers					1070:1077	cell-wall polymers	1060:1077	cell-wall polymers	1060:1077	Association of cell-wall polymers with cellulose driven by entropic amelioration of high energy cellulose/water interfaces should be considered as a third type of interaction within cellulose-based cell walls, in addition to molecular binding (enthalpic driving force) exhibited by, for example, xyloglucans or mannans, and interpenetrating networks based on, for example, pectins.
25756836	1	9	theme	primary	275:281	arg1	walls					288:292	cereal/grass primary cell walls	262:292	cereal/grass primary cell walls	262:292	To identify interactions of relevance to the structure and properties of the primary cell walls of cereals and grasses, we used arabinoxylan and (1,3)(1,4)-β-glucan, major polymers in cereal/grass primary cell walls, to construct composites with cellulose produced by Gluconacetobacter xylinus.
25756836	4	10	theme	cellulose	749:757	arg1	composites					759:768	preformed cellulose composites	739:768	preformed cellulose composites	739:768	In situ depletion of arabinoxylan arabinose side chains within preformed cellulose composites resulted in phase separation, with only limited enhancement of xylan-cellulose interactions.
25756836	5	11	dep	way	1002:1004	arg1	interact					1011:1018	interact	1011:1018	interact with cellulose networks	1011:1042	These results suggest that arabinoxylan and (1 → 3)(1 → 4)-β-d-glucan are not functional homologues for either xyloglucan or pectin in the way they interact with cellulose networks.
25756836	0	12	theme	cellulose	58:66	arg1	networks					68:75	cellulose networks	58:75	cellulose networks	58:75	Interactions of arabinoxylan and (1,3)(1,4)-β-glucan with cellulose networks.
25756836	1	13	theme	cell	283:286	arg1	walls					288:292	cereal/grass primary cell walls	262:292	cereal/grass primary cell walls	262:292	To identify interactions of relevance to the structure and properties of the primary cell walls of cereals and grasses, we used arabinoxylan and (1,3)(1,4)-β-glucan, major polymers in cereal/grass primary cell walls, to construct composites with cellulose produced by Gluconacetobacter xylinus.
25756836	4	14	theme	preformed	739:747	arg1	composites					759:768	preformed cellulose composites	739:768	preformed cellulose composites	739:768	In situ depletion of arabinoxylan arabinose side chains within preformed cellulose composites resulted in phase separation, with only limited enhancement of xylan-cellulose interactions.
25756836	4	15	theme	xylan-cellulose	833:847	arg1	interactions					849:860	xylan-cellulose interactions	833:860	xylan-cellulose interactions	833:860	In situ depletion of arabinoxylan arabinose side chains within preformed cellulose composites resulted in phase separation, with only limited enhancement of xylan-cellulose interactions.
25756836	4	16	theme	side	720:723	arg1	chains					725:730	arabinoxylan arabinose side chains	697:730	arabinoxylan arabinose side chains	697:730	In situ depletion of arabinoxylan arabinose side chains within preformed cellulose composites resulted in phase separation, with only limited enhancement of xylan-cellulose interactions.
25756836	6	17	theme	interfaces	1157:1166	arg1	amelioration					1113:1124	entropic amelioration	1104:1124	entropic amelioration of high energy cellulose/water interfaces	1104:1166	Association of cell-wall polymers with cellulose driven by entropic amelioration of high energy cellulose/water interfaces should be considered as a third type of interaction within cellulose-based cell walls, in addition to molecular binding (enthalpic driving force) exhibited by, for example, xyloglucans or mannans, and interpenetrating networks based on, for example, pectins.
25756836	3	18	dep	xyloglucan	569:578	arg1	components					606:615	characteristic components	591:615	characteristic components of nongrass primary cell walls	591:646	Mechanical properties were modestly affected compared with xyloglucan or pectin (characteristic components of nongrass primary cell walls) composites with cellulose.
25756836	5	19	theme	functional	941:950	arg1	homologues					952:961	functional homologues	941:961	functional homologues for either xyloglucan or pectin	941:993	These results suggest that arabinoxylan and (1 → 3)(1 → 4)-β-d-glucan are not functional homologues for either xyloglucan or pectin in the way they interact with cellulose networks.
25756836	5	19	theme	functional	941:950	arg1	arabinoxylan					890:901	arabinoxylan	890:901	arabinoxylan	890:901	These results suggest that arabinoxylan and (1 → 3)(1 → 4)-β-d-glucan are not functional homologues for either xyloglucan or pectin in the way they interact with cellulose networks.
25756836	5	19	theme	functional	941:950	arg1	-β-d-glucan					921:931	(1 → 3)(1 → 4)-β-d-glucan	907:931	(1 → 3)(1 → 4)-β-d-glucan	907:931	These results suggest that arabinoxylan and (1 → 3)(1 → 4)-β-d-glucan are not functional homologues for either xyloglucan or pectin in the way they interact with cellulose networks.
25756836	5	20	from	homologues	952:961	arg1	way					1002:1004	the way they interact with cellulose networks	998:1042	the way they interact with cellulose networks	998:1042	These results suggest that arabinoxylan and (1 → 3)(1 → 4)-β-d-glucan are not functional homologues for either xyloglucan or pectin in the way they interact with cellulose networks.
25756836	1	21	dep	-β-glucan	233:241	arg1	1,4					229:231	1,4	229:231	1,4	229:231	To identify interactions of relevance to the structure and properties of the primary cell walls of cereals and grasses, we used arabinoxylan and (1,3)(1,4)-β-glucan, major polymers in cereal/grass primary cell walls, to construct composites with cellulose produced by Gluconacetobacter xylinus.
25756836	6	22	theme	cell	1243:1246	arg1	walls					1248:1252	cellulose-based cell walls	1227:1252	cellulose-based cell walls	1227:1252	Association of cell-wall polymers with cellulose driven by entropic amelioration of high energy cellulose/water interfaces should be considered as a third type of interaction within cellulose-based cell walls, in addition to molecular binding (enthalpic driving force) exhibited by, for example, xyloglucans or mannans, and interpenetrating networks based on, for example, pectins.
25756836	1	23	dep	structure	123:131	arg1	the					119:121	the	119:121	the	119:121	To identify interactions of relevance to the structure and properties of the primary cell walls of cereals and grasses, we used arabinoxylan and (1,3)(1,4)-β-glucan, major polymers in cereal/grass primary cell walls, to construct composites with cellulose produced by Gluconacetobacter xylinus.
25756836	3	24	with	composites	649:658	arg1	cellulose					665:673	cellulose	665:673	cellulose	665:673	Mechanical properties were modestly affected compared with xyloglucan or pectin (characteristic components of nongrass primary cell walls) composites with cellulose.
25756836	6	25	dep	binding	1280:1286	arg1	addition					1258:1265	addition	1258:1265	addition	1258:1265	Association of cell-wall polymers with cellulose driven by entropic amelioration of high energy cellulose/water interfaces should be considered as a third type of interaction within cellulose-based cell walls, in addition to molecular binding (enthalpic driving force) exhibited by, for example, xyloglucans or mannans, and interpenetrating networks based on, for example, pectins.
25756836	4	26	theme	interactions	849:860	arg1	enhancement					818:828	only limited enhancement	805:828	only limited enhancement of xylan-cellulose interactions	805:860	In situ depletion of arabinoxylan arabinose side chains within preformed cellulose composites resulted in phase separation, with only limited enhancement of xylan-cellulose interactions.
25756836	0	27	dep	-β-glucan	43:51	arg1	1,4					39:41	1,4	39:41	1,4	39:41	Interactions of arabinoxylan and (1,3)(1,4)-β-glucan with cellulose networks.
25756836	1	28	theme	primary	155:161	arg1	walls					168:172	the primary cell walls	151:172	the primary cell walls of cereals and grasses	151:195	To identify interactions of relevance to the structure and properties of the primary cell walls of cereals and grasses, we used arabinoxylan and (1,3)(1,4)-β-glucan, major polymers in cereal/grass primary cell walls, to construct composites with cellulose produced by Gluconacetobacter xylinus.
25756836	4	29	theme	arabinose	710:718	arg1	chains					725:730	arabinoxylan arabinose side chains	697:730	arabinoxylan arabinose side chains	697:730	In situ depletion of arabinoxylan arabinose side chains within preformed cellulose composites resulted in phase separation, with only limited enhancement of xylan-cellulose interactions.
25756836	2	30	dep	nature	465:470	arg1	the					461:463	the	461:463	the	461:463	Both polymers associated prolifically with cellulose without becoming rigid or altering the nature or extent of cellulose crystallinity.
25756836	3	31	theme	Mechanical	510:519	arg1	properties					521:530	Mechanical properties	510:530	Mechanical properties	510:530	Mechanical properties were modestly affected compared with xyloglucan or pectin (characteristic components of nongrass primary cell walls) composites with cellulose.
25756836	6	32	dep	on	1401:1402	arg1	pectins					1418:1424	pectins	1418:1424	pectins	1418:1424	Association of cell-wall polymers with cellulose driven by entropic amelioration of high energy cellulose/water interfaces should be considered as a third type of interaction within cellulose-based cell walls, in addition to molecular binding (enthalpic driving force) exhibited by, for example, xyloglucans or mannans, and interpenetrating networks based on, for example, pectins.
25756836	6	32	dep	on	1401:1402	arg1	example					1409:1415	example	1409:1415	example	1409:1415	Association of cell-wall polymers with cellulose driven by entropic amelioration of high energy cellulose/water interfaces should be considered as a third type of interaction within cellulose-based cell walls, in addition to molecular binding (enthalpic driving force) exhibited by, for example, xyloglucans or mannans, and interpenetrating networks based on, for example, pectins.
25756836	1	33	theme	cell	163:166	arg1	walls					168:172	the primary cell walls	151:172	the primary cell walls of cereals and grasses	151:195	To identify interactions of relevance to the structure and properties of the primary cell walls of cereals and grasses, we used arabinoxylan and (1,3)(1,4)-β-glucan, major polymers in cereal/grass primary cell walls, to construct composites with cellulose produced by Gluconacetobacter xylinus.
25756836	0	34	theme	arabinoxylan	16:27	arg1	Interactions					0:11	Interactions	0:11	Interactions of arabinoxylan and (1,3)(1,4)-β-glucan with cellulose networks.	0:76	Interactions of arabinoxylan and (1,3)(1,4)-β-glucan with cellulose networks.
25756836	2	35	theme	crystallinity	495:507	arg1	nature					465:470	nature	465:470	nature	465:470	Both polymers associated prolifically with cellulose without becoming rigid or altering the nature or extent of cellulose crystallinity.
25756836	2	35	theme	crystallinity	495:507	arg1	extent					475:480	extent	475:480	extent	475:480	Both polymers associated prolifically with cellulose without becoming rigid or altering the nature or extent of cellulose crystallinity.
25756836	3	36	theme	nongrass	620:627	arg1	walls					642:646	nongrass primary cell walls	620:646	nongrass primary cell walls	620:646	Mechanical properties were modestly affected compared with xyloglucan or pectin (characteristic components of nongrass primary cell walls) composites with cellulose.
25756836	4	37	theme	chains	725:730	arg1	depletion					684:692	In situ depletion	676:692	In situ depletion of arabinoxylan arabinose side chains within preformed cellulose composites	676:768	In situ depletion of arabinoxylan arabinose side chains within preformed cellulose composites resulted in phase separation, with only limited enhancement of xylan-cellulose interactions.
25756836	1	38	theme	walls	168:172	arg1	properties					137:146	properties	137:146	properties	137:146	To identify interactions of relevance to the structure and properties of the primary cell walls of cereals and grasses, we used arabinoxylan and (1,3)(1,4)-β-glucan, major polymers in cereal/grass primary cell walls, to construct composites with cellulose produced by Gluconacetobacter xylinus.
25756836	1	38	theme	walls	168:172	arg1	structure					123:131	structure	123:131	structure	123:131	To identify interactions of relevance to the structure and properties of the primary cell walls of cereals and grasses, we used arabinoxylan and (1,3)(1,4)-β-glucan, major polymers in cereal/grass primary cell walls, to construct composites with cellulose produced by Gluconacetobacter xylinus.
25756836	2	39	theme	cellulose	485:493	arg1	crystallinity					495:507	cellulose crystallinity	485:507	cellulose crystallinity	485:507	Both polymers associated prolifically with cellulose without becoming rigid or altering the nature or extent of cellulose crystallinity.
25756836	3	40	theme	primary	629:635	arg1	walls					642:646	nongrass primary cell walls	620:646	nongrass primary cell walls	620:646	Mechanical properties were modestly affected compared with xyloglucan or pectin (characteristic components of nongrass primary cell walls) composites with cellulose.
25756836	1	41	with	composites	308:317	arg1	cellulose					324:332	cellulose	324:332	cellulose produced by Gluconacetobacter xylinus	324:370	To identify interactions of relevance to the structure and properties of the primary cell walls of cereals and grasses, we used arabinoxylan and (1,3)(1,4)-β-glucan, major polymers in cereal/grass primary cell walls, to construct composites with cellulose produced by Gluconacetobacter xylinus.
25756836	6	42	with	Association	1045:1055	arg1	cellulose					1084:1092	cellulose	1084:1092	cellulose driven by entropic amelioration of high energy cellulose/water interfaces	1084:1166	Association of cell-wall polymers with cellulose driven by entropic amelioration of high energy cellulose/water interfaces should be considered as a third type of interaction within cellulose-based cell walls, in addition to molecular binding (enthalpic driving force) exhibited by, for example, xyloglucans or mannans, and interpenetrating networks based on, for example, pectins.
25756836	3	43	theme	xyloglucan	569:578	arg1	composites					649:658	xyloglucan or pectin (characteristic components of nongrass primary cell walls) composites	569:658	xyloglucan or pectin (characteristic components of nongrass primary cell walls) composites with cellulose	569:673	Mechanical properties were modestly affected compared with xyloglucan or pectin (characteristic components of nongrass primary cell walls) composites with cellulose.
25756836	1	44	theme	cereals	177:183	arg1	walls					168:172	the primary cell walls	151:172	the primary cell walls of cereals and grasses	151:195	To identify interactions of relevance to the structure and properties of the primary cell walls of cereals and grasses, we used arabinoxylan and (1,3)(1,4)-β-glucan, major polymers in cereal/grass primary cell walls, to construct composites with cellulose produced by Gluconacetobacter xylinus.
25756836	1	45	from	polymers	250:257	arg1	walls					288:292	cereal/grass primary cell walls	262:292	cereal/grass primary cell walls	262:292	To identify interactions of relevance to the structure and properties of the primary cell walls of cereals and grasses, we used arabinoxylan and (1,3)(1,4)-β-glucan, major polymers in cereal/grass primary cell walls, to construct composites with cellulose produced by Gluconacetobacter xylinus.
25756836	6	46	theme	driving	1299:1305	arg1	binding					1280:1286	molecular binding	1270:1286	molecular binding (enthalpic driving force) exhibited by, for example, xyloglucans or mannans, and interpenetrating networks based on, for example, pectins	1270:1424	Association of cell-wall polymers with cellulose driven by entropic amelioration of high energy cellulose/water interfaces should be considered as a third type of interaction within cellulose-based cell walls, in addition to molecular binding (enthalpic driving force) exhibited by, for example, xyloglucans or mannans, and interpenetrating networks based on, for example, pectins.
25756836	6	46	theme	driving	1299:1305	arg1	force					1307:1311	enthalpic driving force	1289:1311	enthalpic driving force	1289:1311	Association of cell-wall polymers with cellulose driven by entropic amelioration of high energy cellulose/water interfaces should be considered as a third type of interaction within cellulose-based cell walls, in addition to molecular binding (enthalpic driving force) exhibited by, for example, xyloglucans or mannans, and interpenetrating networks based on, for example, pectins.
25756836	1	47	used	used	201:204	arg2	we					198:199	we	198:199	we	198:199	To identify interactions of relevance to the structure and properties of the primary cell walls of cereals and grasses, we used arabinoxylan and (1,3)(1,4)-β-glucan, major polymers in cereal/grass primary cell walls, to construct composites with cellulose produced by Gluconacetobacter xylinus.
25756836	6	48	theme	entropic	1104:1111	arg1	amelioration					1113:1124	entropic amelioration	1104:1124	entropic amelioration of high energy cellulose/water interfaces	1104:1166	Association of cell-wall polymers with cellulose driven by entropic amelioration of high energy cellulose/water interfaces should be considered as a third type of interaction within cellulose-based cell walls, in addition to molecular binding (enthalpic driving force) exhibited by, for example, xyloglucans or mannans, and interpenetrating networks based on, for example, pectins.
25756836	6	49	theme	interaction	1208:1218	arg1	type					1200:1203	a third type	1192:1203	a third type of interaction within cellulose-based cell walls	1192:1252	Association of cell-wall polymers with cellulose driven by entropic amelioration of high energy cellulose/water interfaces should be considered as a third type of interaction within cellulose-based cell walls, in addition to molecular binding (enthalpic driving force) exhibited by, for example, xyloglucans or mannans, and interpenetrating networks based on, for example, pectins.
25756836	6	49	theme	interaction	1208:1218	arg1	Association					1045:1055	Association	1045:1055	Association of cell-wall polymers with cellulose driven by entropic amelioration of high energy cellulose/water interfaces	1045:1166	Association of cell-wall polymers with cellulose driven by entropic amelioration of high energy cellulose/water interfaces should be considered as a third type of interaction within cellulose-based cell walls, in addition to molecular binding (enthalpic driving force) exhibited by, for example, xyloglucans or mannans, and interpenetrating networks based on, for example, pectins.
25756836	6	50	theme	enthalpic	1289:1297	arg1	binding					1280:1286	molecular binding	1270:1286	molecular binding (enthalpic driving force) exhibited by, for example, xyloglucans or mannans, and interpenetrating networks based on, for example, pectins	1270:1424	Association of cell-wall polymers with cellulose driven by entropic amelioration of high energy cellulose/water interfaces should be considered as a third type of interaction within cellulose-based cell walls, in addition to molecular binding (enthalpic driving force) exhibited by, for example, xyloglucans or mannans, and interpenetrating networks based on, for example, pectins.
25756836	6	50	theme	enthalpic	1289:1297	arg1	force					1307:1311	enthalpic driving force	1289:1311	enthalpic driving force	1289:1311	Association of cell-wall polymers with cellulose driven by entropic amelioration of high energy cellulose/water interfaces should be considered as a third type of interaction within cellulose-based cell walls, in addition to molecular binding (enthalpic driving force) exhibited by, for example, xyloglucans or mannans, and interpenetrating networks based on, for example, pectins.
25756836	4	51	theme	limited	810:816	arg1	enhancement					818:828	only limited enhancement	805:828	only limited enhancement of xylan-cellulose interactions	805:860	In situ depletion of arabinoxylan arabinose side chains within preformed cellulose composites resulted in phase separation, with only limited enhancement of xylan-cellulose interactions.
25756836	1	52	theme	major	244:248	arg1	-β-glucan					233:241	-β-glucan	233:241	-β-glucan	233:241	To identify interactions of relevance to the structure and properties of the primary cell walls of cereals and grasses, we used arabinoxylan and (1,3)(1,4)-β-glucan, major polymers in cereal/grass primary cell walls, to construct composites with cellulose produced by Gluconacetobacter xylinus.
25756836	1	52	theme	major	244:248	arg1	polymers					250:257	major polymers	244:257	major polymers in cereal/grass primary cell walls	244:292	To identify interactions of relevance to the structure and properties of the primary cell walls of cereals and grasses, we used arabinoxylan and (1,3)(1,4)-β-glucan, major polymers in cereal/grass primary cell walls, to construct composites with cellulose produced by Gluconacetobacter xylinus.
25756836	6	53	theme	third	1194:1198	arg1	type					1200:1203	a third type	1192:1203	a third type of interaction within cellulose-based cell walls	1192:1252	Association of cell-wall polymers with cellulose driven by entropic amelioration of high energy cellulose/water interfaces should be considered as a third type of interaction within cellulose-based cell walls, in addition to molecular binding (enthalpic driving force) exhibited by, for example, xyloglucans or mannans, and interpenetrating networks based on, for example, pectins.
25756836	6	53	theme	third	1194:1198	arg1	Association					1045:1055	Association	1045:1055	Association of cell-wall polymers with cellulose driven by entropic amelioration of high energy cellulose/water interfaces	1045:1166	Association of cell-wall polymers with cellulose driven by entropic amelioration of high energy cellulose/water interfaces should be considered as a third type of interaction within cellulose-based cell walls, in addition to molecular binding (enthalpic driving force) exhibited by, for example, xyloglucans or mannans, and interpenetrating networks based on, for example, pectins.
25756836	4	54	theme	phase	782:786	arg1	separation					788:797	phase separation	782:797	phase separation	782:797	In situ depletion of arabinoxylan arabinose side chains within preformed cellulose composites resulted in phase separation, with only limited enhancement of xylan-cellulose interactions.
25756836	0	55	with	Interactions	0:11	arg1	networks					68:75	cellulose networks	58:75	cellulose networks	58:75	Interactions of arabinoxylan and (1,3)(1,4)-β-glucan with cellulose networks.
25756836	4	56	theme	arabinoxylan	697:708	arg1	chains					725:730	arabinoxylan arabinose side chains	697:730	arabinoxylan arabinose side chains	697:730	In situ depletion of arabinoxylan arabinose side chains within preformed cellulose composites resulted in phase separation, with only limited enhancement of xylan-cellulose interactions.
25756836	6	57	theme	cellulose/water	1141:1155	arg1	interfaces					1157:1166	high energy cellulose/water interfaces	1129:1166	high energy cellulose/water interfaces	1129:1166	Association of cell-wall polymers with cellulose driven by entropic amelioration of high energy cellulose/water interfaces should be considered as a third type of interaction within cellulose-based cell walls, in addition to molecular binding (enthalpic driving force) exhibited by, for example, xyloglucans or mannans, and interpenetrating networks based on, for example, pectins.
25756836	1	58	theme	Gluconacetobacter	346:362	arg1	xylinus					364:370	Gluconacetobacter xylinus	346:370	Gluconacetobacter xylinus	346:370	To identify interactions of relevance to the structure and properties of the primary cell walls of cereals and grasses, we used arabinoxylan and (1,3)(1,4)-β-glucan, major polymers in cereal/grass primary cell walls, to construct composites with cellulose produced by Gluconacetobacter xylinus.
25756836	3	59	theme	characteristic	591:604	arg1	components					606:615	characteristic components	591:615	characteristic components of nongrass primary cell walls	591:646	Mechanical properties were modestly affected compared with xyloglucan or pectin (characteristic components of nongrass primary cell walls) composites with cellulose.
25756836	1	60	theme	relevance	106:114	arg1	interactions					90:101	interactions	90:101	interactions of relevance to the structure and properties of the primary cell walls of cereals and grasses	90:195	To identify interactions of relevance to the structure and properties of the primary cell walls of cereals and grasses, we used arabinoxylan and (1,3)(1,4)-β-glucan, major polymers in cereal/grass primary cell walls, to construct composites with cellulose produced by Gluconacetobacter xylinus.
25756836	1	61	theme	grasses	189:195	arg1	walls					168:172	the primary cell walls	151:172	the primary cell walls of cereals and grasses	151:195	To identify interactions of relevance to the structure and properties of the primary cell walls of cereals and grasses, we used arabinoxylan and (1,3)(1,4)-β-glucan, major polymers in cereal/grass primary cell walls, to construct composites with cellulose produced by Gluconacetobacter xylinus.
25756836	3	62	theme	walls	642:646	arg1	components					606:615	characteristic components	591:615	characteristic components of nongrass primary cell walls	591:646	Mechanical properties were modestly affected compared with xyloglucan or pectin (characteristic components of nongrass primary cell walls) composites with cellulose.
25756836	6	63	theme	high	1129:1132	arg1	interfaces					1157:1166	high energy cellulose/water interfaces	1129:1166	high energy cellulose/water interfaces	1129:1166	Association of cell-wall polymers with cellulose driven by entropic amelioration of high energy cellulose/water interfaces should be considered as a third type of interaction within cellulose-based cell walls, in addition to molecular binding (enthalpic driving force) exhibited by, for example, xyloglucans or mannans, and interpenetrating networks based on, for example, pectins.
25756836	6	64	theme	interpenetrating	1369:1384	arg1	networks					1386:1393	interpenetrating networks	1369:1393	interpenetrating networks	1369:1393	Association of cell-wall polymers with cellulose driven by entropic amelioration of high energy cellulose/water interfaces should be considered as a third type of interaction within cellulose-based cell walls, in addition to molecular binding (enthalpic driving force) exhibited by, for example, xyloglucans or mannans, and interpenetrating networks based on, for example, pectins.
25756836	3	65	theme	pectin	583:588	arg1	composites					649:658	xyloglucan or pectin (characteristic components of nongrass primary cell walls) composites	569:658	xyloglucan or pectin (characteristic components of nongrass primary cell walls) composites with cellulose	569:673	Mechanical properties were modestly affected compared with xyloglucan or pectin (characteristic components of nongrass primary cell walls) composites with cellulose.
25756836	6	66	theme	polymers	1070:1077	arg1	type					1200:1203	a third type	1192:1203	a third type of interaction within cellulose-based cell walls	1192:1252	Association of cell-wall polymers with cellulose driven by entropic amelioration of high energy cellulose/water interfaces should be considered as a third type of interaction within cellulose-based cell walls, in addition to molecular binding (enthalpic driving force) exhibited by, for example, xyloglucans or mannans, and interpenetrating networks based on, for example, pectins.
25756836	6	66	theme	polymers	1070:1077	arg1	Association					1045:1055	Association	1045:1055	Association of cell-wall polymers with cellulose driven by entropic amelioration of high energy cellulose/water interfaces	1045:1166	Association of cell-wall polymers with cellulose driven by entropic amelioration of high energy cellulose/water interfaces should be considered as a third type of interaction within cellulose-based cell walls, in addition to molecular binding (enthalpic driving force) exhibited by, for example, xyloglucans or mannans, and interpenetrating networks based on, for example, pectins.
25756836	4	67	dep	In	676:677	arg1	situ					679:682	situ	679:682	situ	679:682	In situ depletion of arabinoxylan arabinose side chains within preformed cellulose composites resulted in phase separation, with only limited enhancement of xylan-cellulose interactions.
27112866	5	0	theme	shift	1001:1005	arg1	values					1007:1012	chemical shift values	992:1012	chemical shift values obtained from a (1→6)/(1→3)-β-d-glucan derived from Saccharomyces cerevisiae which was used as reference	992:1117	Chemical shifts assignments of the α-mannan and β-glucan are presented as well as chemical shift values obtained from a (1→6)/(1→3)-β-d-glucan derived from Saccharomyces cerevisiae which was used as reference.
27112866	5	0	theme	shift	1001:1005	arg1	assignments					926:936	Chemical shifts assignments	910:936	Chemical shifts assignments of the α-mannan and β-glucan	910:965	Chemical shifts assignments of the α-mannan and β-glucan are presented as well as chemical shift values obtained from a (1→6)/(1→3)-β-d-glucan derived from Saccharomyces cerevisiae which was used as reference.
27112866	4	1	theme	NaOH	655:658	arg1	extract					660:666	The hot 1M NaOH extract	644:666	The hot 1M NaOH extract	644:666	The hot 1M NaOH extract contained two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4.
27112866	3	2	theme	short	486:490	arg1	chains					510:515	single and short (1→2)-linked side chains	475:515	single and short (1→2)-linked side chains (WCcF1b)	475:524	A 671kDa (1→6)-linked α-d-mannan with single and short (1→2)-linked side chains (WCcF1b) was isolated from the hot water extract, after protease treatment and fractionation by size exclusion chromatography.
27112866	3	2	theme	short	486:490	arg1	WCcF1b					518:523	WCcF1b	518:523	WCcF1b	518:523	A 671kDa (1→6)-linked α-d-mannan with single and short (1→2)-linked side chains (WCcF1b) was isolated from the hot water extract, after protease treatment and fractionation by size exclusion chromatography.
27112866	0	3	theme	β-glucans	61:69	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization of a branched (1→6)-α-mannan and β-glucans	0:69	Structural characterization of a branched (1→6)-α-mannan and β-glucans isolated from the fruiting bodies of Cantharellus cibarius.
27112866	4	4	theme	side	785:788	arg1	chains					790:795	single and short (1→3)-linked β-glucan side chains	746:795	single and short (1→3)-linked β-glucan side chains (ACcSw)	746:803	The hot 1M NaOH extract contained two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4.
27112866	4	4	theme	side	785:788	arg1	1→3					764:766	1→3	764:766	1→3	764:766	The hot 1M NaOH extract contained two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4.
27112866	4	4	theme	side	785:788	arg1	ACcSw					798:802	ACcSw	798:802	ACcSw	798:802	The hot 1M NaOH extract contained two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4.
27112866	1	5	from	characterization	147:162	arg1	chanterelle					192:202	the chanterelle	188:202	the chanterelle	188:202	A comprehensive characterization of polysaccharides from the chanterelle was performed.
27112866	4	6	theme	1M	652:653	arg1	extract					660:666	The hot 1M NaOH extract	644:666	The hot 1M NaOH extract	644:666	The hot 1M NaOH extract contained two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4.
27112866	4	7	with	β-d-glucan	730:739	arg1	chains					790:795	single and short (1→3)-linked β-glucan side chains	746:795	single and short (1→3)-linked β-glucan side chains (ACcSw)	746:803	The hot 1M NaOH extract contained two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4.
27112866	4	7	with	β-d-glucan	730:739	arg1	1→3					764:766	1→3	764:766	1→3	764:766	The hot 1M NaOH extract contained two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4.
27112866	4	7	with	β-d-glucan	730:739	arg1	ACcSw					798:802	ACcSw	798:802	ACcSw	798:802	The hot 1M NaOH extract contained two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4.
27112866	3	8	link	-linked	497:503	arg1	chains					510:515	single and short (1→2)-linked side chains	475:515	single and short (1→2)-linked side chains (WCcF1b)	475:524	A 671kDa (1→6)-linked α-d-mannan with single and short (1→2)-linked side chains (WCcF1b) was isolated from the hot water extract, after protease treatment and fractionation by size exclusion chromatography.
27112866	3	8	link	-linked	497:503	arg1	WCcF1b					518:523	WCcF1b	518:523	WCcF1b	518:523	A 671kDa (1→6)-linked α-d-mannan with single and short (1→2)-linked side chains (WCcF1b) was isolated from the hot water extract, after protease treatment and fractionation by size exclusion chromatography.
27112866	5	9	used	used	1101:1104	arg2	cerevisiae					1080:1089	Saccharomyces cerevisiae	1066:1089	Saccharomyces cerevisiae which was used as reference	1066:1117	Chemical shifts assignments of the α-mannan and β-glucan are presented as well as chemical shift values obtained from a (1→6)/(1→3)-β-d-glucan derived from Saccharomyces cerevisiae which was used as reference.
27112866	5	9	used	used	1101:1104	arg2	reference					1109:1117	reference	1109:1117	reference	1109:1117	Chemical shifts assignments of the α-mannan and β-glucan are presented as well as chemical shift values obtained from a (1→6)/(1→3)-β-d-glucan derived from Saccharomyces cerevisiae which was used as reference.
27112866	4	10	theme	soluble	709:715	arg1	β-d-glucan					730:739	a water soluble (1→6)-linked β-d-glucan	701:739	a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw)	701:803	The hot 1M NaOH extract contained two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4.
27112866	4	10	theme	soluble	709:715	arg1	types					682:686	two types	678:686	two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4	678:907	The hot 1M NaOH extract contained two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4.
27112866	4	11	theme	-linked	830:836	arg1	ACcIw/ACcId					850:860	ACcIw/ACcId	850:860	ACcIw/ACcId	850:860	The hot 1M NaOH extract contained two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4.
27112866	4	11	theme	-linked	830:836	arg1	β-d-glucan					838:847	a less branched (1→3)-linked β-d-glucan	809:847	a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId)	809:861	The hot 1M NaOH extract contained two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4.
27112866	4	11	theme	-linked	830:836	arg1	types					682:686	two types	678:686	two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4	678:907	The hot 1M NaOH extract contained two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4.
27112866	3	12	link	-linked	451:457	arg1	α-d-mannan					459:468	A 671kDa (1→6)-linked α-d-mannan	437:468	A 671kDa (1→6)-linked α-d-mannan with single and short (1→2)-linked side chains (WCcF1b)	437:524	A 671kDa (1→6)-linked α-d-mannan with single and short (1→2)-linked side chains (WCcF1b) was isolated from the hot water extract, after protease treatment and fractionation by size exclusion chromatography.
27112866	2	13	theme	composition	303:313	arg1	analysis					315:322	monosaccharide composition analysis	288:322	monosaccharide composition analysis by methanolysis	288:338	Experiments included both linkage analysis by methylation and GC-MS, monosaccharide composition analysis by methanolysis, SEC-MALLS and several NMR experiments including COSY, HSQC, TOCSY, HSQC-TOCSY, NOESY, and HMBC.
27112866	3	14	attach	isolated	530:537	arg2	α-d-mannan					459:468	A 671kDa (1→6)-linked α-d-mannan	437:468	A 671kDa (1→6)-linked α-d-mannan with single and short (1→2)-linked side chains (WCcF1b)	437:524	A 671kDa (1→6)-linked α-d-mannan with single and short (1→2)-linked side chains (WCcF1b) was isolated from the hot water extract, after protease treatment and fractionation by size exclusion chromatography.
27112866	3	14	attach	isolated	530:537	arg1	extract					558:564	the hot water extract	544:564	the hot water extract	544:564	A 671kDa (1→6)-linked α-d-mannan with single and short (1→2)-linked side chains (WCcF1b) was isolated from the hot water extract, after protease treatment and fractionation by size exclusion chromatography.
27112866	3	15	with	α-d-mannan	459:468	arg1	chains					510:515	single and short (1→2)-linked side chains	475:515	single and short (1→2)-linked side chains (WCcF1b)	475:524	A 671kDa (1→6)-linked α-d-mannan with single and short (1→2)-linked side chains (WCcF1b) was isolated from the hot water extract, after protease treatment and fractionation by size exclusion chromatography.
27112866	3	15	with	α-d-mannan	459:468	arg1	WCcF1b					518:523	WCcF1b	518:523	WCcF1b	518:523	A 671kDa (1→6)-linked α-d-mannan with single and short (1→2)-linked side chains (WCcF1b) was isolated from the hot water extract, after protease treatment and fractionation by size exclusion chromatography.
27112866	2	16	theme	monosaccharide	288:301	arg1	analysis					315:322	monosaccharide composition analysis	288:322	monosaccharide composition analysis by methanolysis	288:338	Experiments included both linkage analysis by methylation and GC-MS, monosaccharide composition analysis by methanolysis, SEC-MALLS and several NMR experiments including COSY, HSQC, TOCSY, HSQC-TOCSY, NOESY, and HMBC.
27112866	1	17	theme	polysaccharides	167:181	arg1	characterization					147:162	A comprehensive characterization	131:162	A comprehensive characterization of polysaccharides from the chanterelle	131:202	A comprehensive characterization of polysaccharides from the chanterelle was performed.
27112866	0	18	theme	fruiting	89:96	arg1	bodies					98:103	the fruiting bodies	85:103	the fruiting bodies of Cantharellus cibarius	85:128	Structural characterization of a branched (1→6)-α-mannan and β-glucans isolated from the fruiting bodies of Cantharellus cibarius.
27112866	3	19	theme	side	505:508	arg1	chains					510:515	single and short (1→2)-linked side chains	475:515	single and short (1→2)-linked side chains (WCcF1b)	475:524	A 671kDa (1→6)-linked α-d-mannan with single and short (1→2)-linked side chains (WCcF1b) was isolated from the hot water extract, after protease treatment and fractionation by size exclusion chromatography.
27112866	3	19	theme	side	505:508	arg1	WCcF1b					518:523	WCcF1b	518:523	WCcF1b	518:523	A 671kDa (1→6)-linked α-d-mannan with single and short (1→2)-linked side chains (WCcF1b) was isolated from the hot water extract, after protease treatment and fractionation by size exclusion chromatography.
27112866	4	20	contain	contained	668:676	arg2	types					682:686	two types	678:686	two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4	678:907	The hot 1M NaOH extract contained two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4.
27112866	4	20	contain	contained	668:676	arg2	β-d-glucan					838:847	a less branched (1→3)-linked β-d-glucan	809:847	a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId)	809:861	The hot 1M NaOH extract contained two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4.
27112866	4	20	contain	contained	668:676	arg1	extract					660:666	The hot 1M NaOH extract	644:666	The hot 1M NaOH extract	644:666	The hot 1M NaOH extract contained two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4.
27112866	4	20	contain	contained	668:676	arg2	β-d-glucan					730:739	a water soluble (1→6)-linked β-d-glucan	701:739	a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw)	701:803	The hot 1M NaOH extract contained two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4.
27112866	4	21	theme	β-glucan	776:783	arg1	chains					790:795	single and short (1→3)-linked β-glucan side chains	746:795	single and short (1→3)-linked β-glucan side chains (ACcSw)	746:803	The hot 1M NaOH extract contained two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4.
27112866	4	21	theme	β-glucan	776:783	arg1	1→3					764:766	1→3	764:766	1→3	764:766	The hot 1M NaOH extract contained two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4.
27112866	4	21	theme	β-glucan	776:783	arg1	ACcSw					798:802	ACcSw	798:802	ACcSw	798:802	The hot 1M NaOH extract contained two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4.
27112866	4	22	theme	β-glucan	691:698	arg1	β-d-glucan					730:739	a water soluble (1→6)-linked β-d-glucan	701:739	a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw)	701:803	The hot 1M NaOH extract contained two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4.
27112866	4	22	theme	β-glucan	691:698	arg1	β-d-glucan					838:847	a less branched (1→3)-linked β-d-glucan	809:847	a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId)	809:861	The hot 1M NaOH extract contained two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4.
27112866	4	22	theme	β-glucan	691:698	arg1	types					682:686	two types	678:686	two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4	678:907	The hot 1M NaOH extract contained two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4.
27112866	0	23	theme	Structural	0:9	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization of a branched (1→6)-α-mannan and β-glucans	0:69	Structural characterization of a branched (1→6)-α-mannan and β-glucans isolated from the fruiting bodies of Cantharellus cibarius.
27112866	4	24	theme	-linked	768:774	arg1	chains					790:795	single and short (1→3)-linked β-glucan side chains	746:795	single and short (1→3)-linked β-glucan side chains (ACcSw)	746:803	The hot 1M NaOH extract contained two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4.
27112866	4	24	theme	-linked	768:774	arg1	1→3					764:766	1→3	764:766	1→3	764:766	The hot 1M NaOH extract contained two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4.
27112866	4	24	theme	-linked	768:774	arg1	ACcSw					798:802	ACcSw	798:802	ACcSw	798:802	The hot 1M NaOH extract contained two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4.
27112866	5	25	theme	Chemical	910:917	arg1	shifts					919:924	Chemical shifts	910:924	Chemical shifts assignments of the α-mannan and β-glucan	910:965	Chemical shifts assignments of the α-mannan and β-glucan are presented as well as chemical shift values obtained from a (1→6)/(1→3)-β-d-glucan derived from Saccharomyces cerevisiae which was used as reference.
27112866	3	26	theme	-linked	497:503	arg1	chains					510:515	single and short (1→2)-linked side chains	475:515	single and short (1→2)-linked side chains (WCcF1b)	475:524	A 671kDa (1→6)-linked α-d-mannan with single and short (1→2)-linked side chains (WCcF1b) was isolated from the hot water extract, after protease treatment and fractionation by size exclusion chromatography.
27112866	3	26	theme	-linked	497:503	arg1	WCcF1b					518:523	WCcF1b	518:523	WCcF1b	518:523	A 671kDa (1→6)-linked α-d-mannan with single and short (1→2)-linked side chains (WCcF1b) was isolated from the hot water extract, after protease treatment and fractionation by size exclusion chromatography.
27112866	4	27	dep	soluble	709:715	arg1	1→6					718:720	1→6	718:720	1→6	718:720	The hot 1M NaOH extract contained two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4.
27112866	3	28	theme	-linked	451:457	arg1	α-d-mannan					459:468	A 671kDa (1→6)-linked α-d-mannan	437:468	A 671kDa (1→6)-linked α-d-mannan with single and short (1→2)-linked side chains (WCcF1b)	437:524	A 671kDa (1→6)-linked α-d-mannan with single and short (1→2)-linked side chains (WCcF1b) was isolated from the hot water extract, after protease treatment and fractionation by size exclusion chromatography.
27112866	4	29	theme	short	884:888	arg1	chains					895:900	short side chains	884:900	short side chains	884:900	The hot 1M NaOH extract contained two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4.
27112866	3	30	theme	hot	548:550	arg1	extract					558:564	the hot water extract	544:564	the hot water extract	544:564	A 671kDa (1→6)-linked α-d-mannan with single and short (1→2)-linked side chains (WCcF1b) was isolated from the hot water extract, after protease treatment and fractionation by size exclusion chromatography.
27112866	4	31	link	-linked	722:728	arg1	β-d-glucan					730:739	a water soluble (1→6)-linked β-d-glucan	701:739	a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw)	701:803	The hot 1M NaOH extract contained two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4.
27112866	4	31	link	-linked	722:728	arg1	types					682:686	two types	678:686	two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4	678:907	The hot 1M NaOH extract contained two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4.
27112866	0	32	theme	branched	33:40	arg1	-α-mannan					47:55	a branched (1→6)-α-mannan	31:55	a branched (1→6)-α-mannan	31:55	Structural characterization of a branched (1→6)-α-mannan and β-glucans isolated from the fruiting bodies of Cantharellus cibarius.
27112866	0	32	theme	branched	33:40	arg1	1→6					43:45	1→6	43:45	1→6	43:45	Structural characterization of a branched (1→6)-α-mannan and β-glucans isolated from the fruiting bodies of Cantharellus cibarius.
27112866	0	33	theme	cibarius	121:128	arg1	bodies					98:103	the fruiting bodies	85:103	the fruiting bodies of Cantharellus cibarius	85:128	Structural characterization of a branched (1→6)-α-mannan and β-glucans isolated from the fruiting bodies of Cantharellus cibarius.
27112866	5	34	theme	β-glucan	958:965	arg1	values					1007:1012	chemical shift values	992:1012	chemical shift values obtained from a (1→6)/(1→3)-β-d-glucan derived from Saccharomyces cerevisiae which was used as reference	992:1117	Chemical shifts assignments of the α-mannan and β-glucan are presented as well as chemical shift values obtained from a (1→6)/(1→3)-β-d-glucan derived from Saccharomyces cerevisiae which was used as reference.
27112866	5	34	theme	β-glucan	958:965	arg1	assignments					926:936	Chemical shifts assignments	910:936	Chemical shifts assignments of the α-mannan and β-glucan	910:965	Chemical shifts assignments of the α-mannan and β-glucan are presented as well as chemical shift values obtained from a (1→6)/(1→3)-β-d-glucan derived from Saccharomyces cerevisiae which was used as reference.
27112866	3	35	theme	water	552:556	arg1	extract					558:564	the hot water extract	544:564	the hot water extract	544:564	A 671kDa (1→6)-linked α-d-mannan with single and short (1→2)-linked side chains (WCcF1b) was isolated from the hot water extract, after protease treatment and fractionation by size exclusion chromatography.
27112866	4	36	dep	branched	816:823	arg1	1→3					826:828	1→3	826:828	1→3	826:828	The hot 1M NaOH extract contained two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4.
27112866	5	37	theme	α-mannan	945:952	arg1	values					1007:1012	chemical shift values	992:1012	chemical shift values obtained from a (1→6)/(1→3)-β-d-glucan derived from Saccharomyces cerevisiae which was used as reference	992:1117	Chemical shifts assignments of the α-mannan and β-glucan are presented as well as chemical shift values obtained from a (1→6)/(1→3)-β-d-glucan derived from Saccharomyces cerevisiae which was used as reference.
27112866	5	37	theme	α-mannan	945:952	arg1	assignments					926:936	Chemical shifts assignments	910:936	Chemical shifts assignments of the α-mannan and β-glucan	910:965	Chemical shifts assignments of the α-mannan and β-glucan are presented as well as chemical shift values obtained from a (1→6)/(1→3)-β-d-glucan derived from Saccharomyces cerevisiae which was used as reference.
27112866	5	38	theme	a	1028:1028	arg1	-β-d-glucan					1041:1051	a (1→6)/(1→3)-β-d-glucan	1028:1051	a (1→6)/(1→3)-β-d-glucan derived from Saccharomyces cerevisiae which was used as reference	1028:1117	Chemical shifts assignments of the α-mannan and β-glucan are presented as well as chemical shift values obtained from a (1→6)/(1→3)-β-d-glucan derived from Saccharomyces cerevisiae which was used as reference.
27112866	3	39	theme	single	475:480	arg1	chains					510:515	single and short (1→2)-linked side chains	475:515	single and short (1→2)-linked side chains (WCcF1b)	475:524	A 671kDa (1→6)-linked α-d-mannan with single and short (1→2)-linked side chains (WCcF1b) was isolated from the hot water extract, after protease treatment and fractionation by size exclusion chromatography.
27112866	3	39	theme	single	475:480	arg1	WCcF1b					518:523	WCcF1b	518:523	WCcF1b	518:523	A 671kDa (1→6)-linked α-d-mannan with single and short (1→2)-linked side chains (WCcF1b) was isolated from the hot water extract, after protease treatment and fractionation by size exclusion chromatography.
27112866	4	40	theme	side	890:893	arg1	chains					895:900	short side chains	884:900	short side chains	884:900	The hot 1M NaOH extract contained two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4.
27112866	5	41	theme	shifts	919:924	arg1	values					1007:1012	chemical shift values	992:1012	chemical shift values obtained from a (1→6)/(1→3)-β-d-glucan derived from Saccharomyces cerevisiae which was used as reference	992:1117	Chemical shifts assignments of the α-mannan and β-glucan are presented as well as chemical shift values obtained from a (1→6)/(1→3)-β-d-glucan derived from Saccharomyces cerevisiae which was used as reference.
27112866	5	41	theme	shifts	919:924	arg1	assignments					926:936	Chemical shifts assignments	910:936	Chemical shifts assignments of the α-mannan and β-glucan	910:965	Chemical shifts assignments of the α-mannan and β-glucan are presented as well as chemical shift values obtained from a (1→6)/(1→3)-β-d-glucan derived from Saccharomyces cerevisiae which was used as reference.
27112866	4	42	theme	short	757:761	arg1	chains					790:795	single and short (1→3)-linked β-glucan side chains	746:795	single and short (1→3)-linked β-glucan side chains (ACcSw)	746:803	The hot 1M NaOH extract contained two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4.
27112866	4	42	theme	short	757:761	arg1	1→3					764:766	1→3	764:766	1→3	764:766	The hot 1M NaOH extract contained two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4.
27112866	4	42	theme	short	757:761	arg1	ACcSw					798:802	ACcSw	798:802	ACcSw	798:802	The hot 1M NaOH extract contained two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4.
27112866	2	43	theme	NMR	363:365	arg1	HMBC					431:434	HMBC	431:434	HMBC	431:434	Experiments included both linkage analysis by methylation and GC-MS, monosaccharide composition analysis by methanolysis, SEC-MALLS and several NMR experiments including COSY, HSQC, TOCSY, HSQC-TOCSY, NOESY, and HMBC.
27112866	2	43	theme	NMR	363:365	arg1	HSQC-TOCSY					408:417	HSQC-TOCSY	408:417	HSQC-TOCSY	408:417	Experiments included both linkage analysis by methylation and GC-MS, monosaccharide composition analysis by methanolysis, SEC-MALLS and several NMR experiments including COSY, HSQC, TOCSY, HSQC-TOCSY, NOESY, and HMBC.
27112866	2	43	theme	NMR	363:365	arg1	COSY					389:392	COSY	389:392	COSY	389:392	Experiments included both linkage analysis by methylation and GC-MS, monosaccharide composition analysis by methanolysis, SEC-MALLS and several NMR experiments including COSY, HSQC, TOCSY, HSQC-TOCSY, NOESY, and HMBC.
27112866	2	43	theme	NMR	363:365	arg1	HSQC					395:398	HSQC	395:398	HSQC	395:398	Experiments included both linkage analysis by methylation and GC-MS, monosaccharide composition analysis by methanolysis, SEC-MALLS and several NMR experiments including COSY, HSQC, TOCSY, HSQC-TOCSY, NOESY, and HMBC.
27112866	2	43	theme	NMR	363:365	arg1	NOESY					420:424	NOESY	420:424	NOESY	420:424	Experiments included both linkage analysis by methylation and GC-MS, monosaccharide composition analysis by methanolysis, SEC-MALLS and several NMR experiments including COSY, HSQC, TOCSY, HSQC-TOCSY, NOESY, and HMBC.
27112866	2	43	theme	NMR	363:365	arg1	experiments					367:377	several NMR experiments	355:377	several NMR experiments including COSY, HSQC, TOCSY, HSQC-TOCSY, NOESY, and HMBC	355:434	Experiments included both linkage analysis by methylation and GC-MS, monosaccharide composition analysis by methanolysis, SEC-MALLS and several NMR experiments including COSY, HSQC, TOCSY, HSQC-TOCSY, NOESY, and HMBC.
27112866	2	43	theme	NMR	363:365	arg1	TOCSY					401:405	TOCSY	401:405	TOCSY	401:405	Experiments included both linkage analysis by methylation and GC-MS, monosaccharide composition analysis by methanolysis, SEC-MALLS and several NMR experiments including COSY, HSQC, TOCSY, HSQC-TOCSY, NOESY, and HMBC.
27112866	4	44	theme	single	746:751	arg1	chains					790:795	single and short (1→3)-linked β-glucan side chains	746:795	single and short (1→3)-linked β-glucan side chains (ACcSw)	746:803	The hot 1M NaOH extract contained two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4.
27112866	4	44	theme	single	746:751	arg1	1→3					764:766	1→3	764:766	1→3	764:766	The hot 1M NaOH extract contained two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4.
27112866	4	44	theme	single	746:751	arg1	ACcSw					798:802	ACcSw	798:802	ACcSw	798:802	The hot 1M NaOH extract contained two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4.
27112866	4	45	theme	-linked	722:728	arg1	β-d-glucan					730:739	a water soluble (1→6)-linked β-d-glucan	701:739	a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw)	701:803	The hot 1M NaOH extract contained two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4.
27112866	4	45	theme	-linked	722:728	arg1	types					682:686	two types	678:686	two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4	678:907	The hot 1M NaOH extract contained two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4.
27112866	2	46	theme	linkage	245:251	arg1	analysis					253:260	both linkage analysis	240:260	both linkage analysis by methylation and GC-MS	240:285	Experiments included both linkage analysis by methylation and GC-MS, monosaccharide composition analysis by methanolysis, SEC-MALLS and several NMR experiments including COSY, HSQC, TOCSY, HSQC-TOCSY, NOESY, and HMBC.
27112866	2	47	theme	several	355:361	arg1	HMBC					431:434	HMBC	431:434	HMBC	431:434	Experiments included both linkage analysis by methylation and GC-MS, monosaccharide composition analysis by methanolysis, SEC-MALLS and several NMR experiments including COSY, HSQC, TOCSY, HSQC-TOCSY, NOESY, and HMBC.
27112866	2	47	theme	several	355:361	arg1	HSQC-TOCSY					408:417	HSQC-TOCSY	408:417	HSQC-TOCSY	408:417	Experiments included both linkage analysis by methylation and GC-MS, monosaccharide composition analysis by methanolysis, SEC-MALLS and several NMR experiments including COSY, HSQC, TOCSY, HSQC-TOCSY, NOESY, and HMBC.
27112866	2	47	theme	several	355:361	arg1	COSY					389:392	COSY	389:392	COSY	389:392	Experiments included both linkage analysis by methylation and GC-MS, monosaccharide composition analysis by methanolysis, SEC-MALLS and several NMR experiments including COSY, HSQC, TOCSY, HSQC-TOCSY, NOESY, and HMBC.
27112866	2	47	theme	several	355:361	arg1	HSQC					395:398	HSQC	395:398	HSQC	395:398	Experiments included both linkage analysis by methylation and GC-MS, monosaccharide composition analysis by methanolysis, SEC-MALLS and several NMR experiments including COSY, HSQC, TOCSY, HSQC-TOCSY, NOESY, and HMBC.
27112866	2	47	theme	several	355:361	arg1	NOESY					420:424	NOESY	420:424	NOESY	420:424	Experiments included both linkage analysis by methylation and GC-MS, monosaccharide composition analysis by methanolysis, SEC-MALLS and several NMR experiments including COSY, HSQC, TOCSY, HSQC-TOCSY, NOESY, and HMBC.
27112866	2	47	theme	several	355:361	arg1	experiments					367:377	several NMR experiments	355:377	several NMR experiments including COSY, HSQC, TOCSY, HSQC-TOCSY, NOESY, and HMBC	355:434	Experiments included both linkage analysis by methylation and GC-MS, monosaccharide composition analysis by methanolysis, SEC-MALLS and several NMR experiments including COSY, HSQC, TOCSY, HSQC-TOCSY, NOESY, and HMBC.
27112866	2	47	theme	several	355:361	arg1	TOCSY					401:405	TOCSY	401:405	TOCSY	401:405	Experiments included both linkage analysis by methylation and GC-MS, monosaccharide composition analysis by methanolysis, SEC-MALLS and several NMR experiments including COSY, HSQC, TOCSY, HSQC-TOCSY, NOESY, and HMBC.
27112866	4	48	dep	types	682:686	arg1	β-d-glucan					730:739	a water soluble (1→6)-linked β-d-glucan	701:739	a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw)	701:803	The hot 1M NaOH extract contained two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4.
27112866	4	48	dep	types	682:686	arg1	ACcIw/ACcId					850:860	ACcIw/ACcId	850:860	ACcIw/ACcId	850:860	The hot 1M NaOH extract contained two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4.
27112866	4	48	dep	types	682:686	arg1	β-d-glucan					838:847	a less branched (1→3)-linked β-d-glucan	809:847	a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId)	809:861	The hot 1M NaOH extract contained two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4.
27112866	4	48	dep	types	682:686	arg1	types					682:686	two types	678:686	two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4	678:907	The hot 1M NaOH extract contained two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4.
27112866	4	49	link	-linked	830:836	arg1	ACcIw/ACcId					850:860	ACcIw/ACcId	850:860	ACcIw/ACcId	850:860	The hot 1M NaOH extract contained two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4.
27112866	4	49	link	-linked	830:836	arg1	β-d-glucan					838:847	a less branched (1→3)-linked β-d-glucan	809:847	a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId)	809:861	The hot 1M NaOH extract contained two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4.
27112866	4	49	link	-linked	830:836	arg1	types					682:686	two types	678:686	two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4	678:907	The hot 1M NaOH extract contained two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4.
27112866	3	50	theme	size	613:616	arg1	chromatography					628:641	size exclusion chromatography	613:641	size exclusion chromatography	613:641	A 671kDa (1→6)-linked α-d-mannan with single and short (1→2)-linked side chains (WCcF1b) was isolated from the hot water extract, after protease treatment and fractionation by size exclusion chromatography.
27112866	3	51	theme	protease	573:580	arg1	treatment					582:590	protease treatment	573:590	protease treatment	573:590	A 671kDa (1→6)-linked α-d-mannan with single and short (1→2)-linked side chains (WCcF1b) was isolated from the hot water extract, after protease treatment and fractionation by size exclusion chromatography.
27112866	4	52	theme	branched	816:823	arg1	ACcIw/ACcId					850:860	ACcIw/ACcId	850:860	ACcIw/ACcId	850:860	The hot 1M NaOH extract contained two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4.
27112866	4	52	theme	branched	816:823	arg1	β-d-glucan					838:847	a less branched (1→3)-linked β-d-glucan	809:847	a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId)	809:861	The hot 1M NaOH extract contained two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4.
27112866	4	52	theme	branched	816:823	arg1	types					682:686	two types	678:686	two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4	678:907	The hot 1M NaOH extract contained two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4.
27112866	3	53	theme	exclusion	618:626	arg1	chromatography					628:641	size exclusion chromatography	613:641	size exclusion chromatography	613:641	A 671kDa (1→6)-linked α-d-mannan with single and short (1→2)-linked side chains (WCcF1b) was isolated from the hot water extract, after protease treatment and fractionation by size exclusion chromatography.
27112866	1	54	from	chanterelle	192:202	arg1	polysaccharides					167:181	polysaccharides	167:181	polysaccharides from the chanterelle	167:202	A comprehensive characterization of polysaccharides from the chanterelle was performed.
27112866	1	54	from	chanterelle	192:202	arg1	characterization					147:162	A comprehensive characterization	131:162	A comprehensive characterization of polysaccharides from the chanterelle	131:202	A comprehensive characterization of polysaccharides from the chanterelle was performed.
27112866	4	55	theme	hot	648:650	arg1	extract					660:666	The hot 1M NaOH extract	644:666	The hot 1M NaOH extract	644:666	The hot 1M NaOH extract contained two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4.
27112866	5	56	dep	α-mannan	945:952	arg1	the					941:943	the	941:943	the	941:943	Chemical shifts assignments of the α-mannan and β-glucan are presented as well as chemical shift values obtained from a (1→6)/(1→3)-β-d-glucan derived from Saccharomyces cerevisiae which was used as reference.
27112866	0	57	theme	-α-mannan	47:55	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization of a branched (1→6)-α-mannan and β-glucans	0:69	Structural characterization of a branched (1→6)-α-mannan and β-glucans isolated from the fruiting bodies of Cantharellus cibarius.
27112866	5	58	theme	chemical	992:999	arg1	values					1007:1012	chemical shift values	992:1012	chemical shift values obtained from a (1→6)/(1→3)-β-d-glucan derived from Saccharomyces cerevisiae which was used as reference	992:1117	Chemical shifts assignments of the α-mannan and β-glucan are presented as well as chemical shift values obtained from a (1→6)/(1→3)-β-d-glucan derived from Saccharomyces cerevisiae which was used as reference.
27112866	5	58	theme	chemical	992:999	arg1	assignments					926:936	Chemical shifts assignments	910:936	Chemical shifts assignments of the α-mannan and β-glucan	910:965	Chemical shifts assignments of the α-mannan and β-glucan are presented as well as chemical shift values obtained from a (1→6)/(1→3)-β-d-glucan derived from Saccharomyces cerevisiae which was used as reference.
27112866	5	59	attach	derived	1053:1059	arg1	reference					1109:1117	reference	1109:1117	reference	1109:1117	Chemical shifts assignments of the α-mannan and β-glucan are presented as well as chemical shift values obtained from a (1→6)/(1→3)-β-d-glucan derived from Saccharomyces cerevisiae which was used as reference.
27112866	5	59	attach	derived	1053:1059	arg1	cerevisiae					1080:1089	Saccharomyces cerevisiae	1066:1089	Saccharomyces cerevisiae which was used as reference	1066:1117	Chemical shifts assignments of the α-mannan and β-glucan are presented as well as chemical shift values obtained from a (1→6)/(1→3)-β-d-glucan derived from Saccharomyces cerevisiae which was used as reference.
27112866	5	59	attach	derived	1053:1059	arg2	-β-d-glucan					1041:1051	a (1→6)/(1→3)-β-d-glucan	1028:1051	a (1→6)/(1→3)-β-d-glucan derived from Saccharomyces cerevisiae which was used as reference	1028:1117	Chemical shifts assignments of the α-mannan and β-glucan are presented as well as chemical shift values obtained from a (1→6)/(1→3)-β-d-glucan derived from Saccharomyces cerevisiae which was used as reference.
27112866	4	60	with	β-d-glucan	838:847	arg1	chains					790:795	single and short (1→3)-linked β-glucan side chains	746:795	single and short (1→3)-linked β-glucan side chains (ACcSw)	746:803	The hot 1M NaOH extract contained two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4.
27112866	4	60	with	β-d-glucan	838:847	arg1	1→3					764:766	1→3	764:766	1→3	764:766	The hot 1M NaOH extract contained two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4.
27112866	4	60	with	β-d-glucan	838:847	arg1	ACcSw					798:802	ACcSw	798:802	ACcSw	798:802	The hot 1M NaOH extract contained two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4.
27112866	1	61	theme	comprehensive	133:145	arg1	characterization					147:162	A comprehensive characterization	131:162	A comprehensive characterization of polysaccharides from the chanterelle	131:202	A comprehensive characterization of polysaccharides from the chanterelle was performed.
27112866	4	62	link	-linked	768:774	arg1	chains					790:795	single and short (1→3)-linked β-glucan side chains	746:795	single and short (1→3)-linked β-glucan side chains (ACcSw)	746:803	The hot 1M NaOH extract contained two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4.
27112866	4	62	link	-linked	768:774	arg1	1→3					764:766	1→3	764:766	1→3	764:766	The hot 1M NaOH extract contained two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4.
27112866	4	62	link	-linked	768:774	arg1	ACcSw					798:802	ACcSw	798:802	ACcSw	798:802	The hot 1M NaOH extract contained two types of β-glucan; a water soluble (1→6)-linked β-d-glucan with single and short (1→3)-linked β-glucan side chains (ACcSw) and a less branched (1→3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4.
29430281	5	0	theme	liver	1203:1207	arg1	diseases					1209:1216	other liver diseases	1197:1216	other liver diseases	1197:1216	These results may provide references for the exploitation of the SMS of L. sulphureus as a source of H-SMPS and E-SMPS, which in turn can be used as functional foods or natural drugs for the prevention of ALD and other liver diseases.
29430281	2	1	theme	scavenging	497:506	arg1	activities					508:517	potential scavenging activities	487:517	potential scavenging activities	487:517	Both H-SMPS and E-SMPS were found to have high reducing power and potential scavenging activities against hydroxyl, DPPH, and superoxide anion radicals.
29430281	1	2	theme	sulphureus	257:266	arg1	utilization					196:206	utilization	196:206	utilization	196:206	In order to contribute to the exploitation and utilization of spent mushroom substrates (SMS) of Laetiporus sulphureus, hot-water-extractable polysaccharides (H-SMPS) and enzymatic-extractable polysaccharides (E-SMPS) were successfully isolated from SMS of L. sulphureus.
29430281	1	2	theme	sulphureus	257:266	arg1	exploitation					179:190	exploitation	179:190	exploitation	179:190	In order to contribute to the exploitation and utilization of spent mushroom substrates (SMS) of Laetiporus sulphureus, hot-water-extractable polysaccharides (H-SMPS) and enzymatic-extractable polysaccharides (E-SMPS) were successfully isolated from SMS of L. sulphureus.
29430281	1	2	theme	sulphureus	257:266	arg1	SMS					238:240	SMS	238:240	SMS	238:240	In order to contribute to the exploitation and utilization of spent mushroom substrates (SMS) of Laetiporus sulphureus, hot-water-extractable polysaccharides (H-SMPS) and enzymatic-extractable polysaccharides (E-SMPS) were successfully isolated from SMS of L. sulphureus.
29430281	4	3	theme	stronger	957:964	arg1	hepatoprotection					966:981	stronger hepatoprotection	957:981	stronger hepatoprotection	957:981	Furthermore, monosaccharide composition analysis showed that fucose may play a vital role in guaranteeing stronger hepatoprotection.
29430281	0	4	from	Antioxidant	0:10	arg1	Mice					143:146	Acute Alcohol-Induced Mice	121:146	Acute Alcohol-Induced Mice	121:146	Antioxidant and Hepatoprotective Activities of Polysaccharides from Spent Mushroom Substrates (Laetiporus sulphureus) in Acute Alcohol-Induced Mice.
29430281	0	4	from	Antioxidant	0:10	arg1	Substrates					83:92	Spent Mushroom Substrates	68:92	Spent Mushroom Substrates (Laetiporus sulphureus)	68:116	Antioxidant and Hepatoprotective Activities of Polysaccharides from Spent Mushroom Substrates (Laetiporus sulphureus) in Acute Alcohol-Induced Mice.
29430281	5	5	theme	functional	1133:1142	arg1	foods					1144:1148	functional foods	1133:1148	functional foods	1133:1148	These results may provide references for the exploitation of the SMS of L. sulphureus as a source of H-SMPS and E-SMPS, which in turn can be used as functional foods or natural drugs for the prevention of ALD and other liver diseases.
29430281	5	5	theme	functional	1133:1142	arg1	source					1075:1080	a source	1073:1080	a source	1073:1080	These results may provide references for the exploitation of the SMS of L. sulphureus as a source of H-SMPS and E-SMPS, which in turn can be used as functional foods or natural drugs for the prevention of ALD and other liver diseases.
29430281	5	6	theme	natural	1153:1159	arg1	drugs					1161:1165	natural drugs	1153:1165	natural drugs	1153:1165	These results may provide references for the exploitation of the SMS of L. sulphureus as a source of H-SMPS and E-SMPS, which in turn can be used as functional foods or natural drugs for the prevention of ALD and other liver diseases.
29430281	5	6	theme	natural	1153:1159	arg1	source					1075:1080	a source	1073:1080	a source	1073:1080	These results may provide references for the exploitation of the SMS of L. sulphureus as a source of H-SMPS and E-SMPS, which in turn can be used as functional foods or natural drugs for the prevention of ALD and other liver diseases.
29430281	2	7	contain	have	458:461	arg2	power					477:481	high reducing power	463:481	high reducing power	463:481	Both H-SMPS and E-SMPS were found to have high reducing power and potential scavenging activities against hydroxyl, DPPH, and superoxide anion radicals.
29430281	2	7	contain	have	458:461	arg1	E-SMPS					437:442	E-SMPS	437:442	E-SMPS	437:442	Both H-SMPS and E-SMPS were found to have high reducing power and potential scavenging activities against hydroxyl, DPPH, and superoxide anion radicals.
29430281	2	7	contain	have	458:461	arg2	activities					508:517	potential scavenging activities	487:517	potential scavenging activities	487:517	Both H-SMPS and E-SMPS were found to have high reducing power and potential scavenging activities against hydroxyl, DPPH, and superoxide anion radicals.
29430281	2	7	contain	have	458:461	arg1	H-SMPS					426:431	H-SMPS	426:431	H-SMPS	426:431	Both H-SMPS and E-SMPS were found to have high reducing power and potential scavenging activities against hydroxyl, DPPH, and superoxide anion radicals.
29430281	1	8	theme	hot-water-extractable	269:289	arg1	H-SMPS					308:313	H-SMPS	308:313	H-SMPS	308:313	In order to contribute to the exploitation and utilization of spent mushroom substrates (SMS) of Laetiporus sulphureus, hot-water-extractable polysaccharides (H-SMPS) and enzymatic-extractable polysaccharides (E-SMPS) were successfully isolated from SMS of L. sulphureus.
29430281	1	8	theme	hot-water-extractable	269:289	arg1	polysaccharides					291:305	hot-water-extractable polysaccharides	269:305	hot-water-extractable polysaccharides (H-SMPS)	269:314	In order to contribute to the exploitation and utilization of spent mushroom substrates (SMS) of Laetiporus sulphureus, hot-water-extractable polysaccharides (H-SMPS) and enzymatic-extractable polysaccharides (E-SMPS) were successfully isolated from SMS of L. sulphureus.
29430281	0	9	theme	Acute	121:125	arg1	Mice					143:146	Acute Alcohol-Induced Mice	121:146	Acute Alcohol-Induced Mice	121:146	Antioxidant and Hepatoprotective Activities of Polysaccharides from Spent Mushroom Substrates (Laetiporus sulphureus) in Acute Alcohol-Induced Mice.
29430281	2	10	theme	potential	487:495	arg1	activities					508:517	potential scavenging activities	487:517	potential scavenging activities	487:517	Both H-SMPS and E-SMPS were found to have high reducing power and potential scavenging activities against hydroxyl, DPPH, and superoxide anion radicals.
29430281	2	11	theme	high	463:466	arg1	power					477:481	high reducing power	463:481	high reducing power	463:481	Both H-SMPS and E-SMPS were found to have high reducing power and potential scavenging activities against hydroxyl, DPPH, and superoxide anion radicals.
29430281	5	12	theme	other	1197:1201	arg1	diseases					1209:1216	other liver diseases	1197:1216	other liver diseases	1197:1216	These results may provide references for the exploitation of the SMS of L. sulphureus as a source of H-SMPS and E-SMPS, which in turn can be used as functional foods or natural drugs for the prevention of ALD and other liver diseases.
29430281	5	13	theme	E-SMPS	1096:1101	arg1	foods					1144:1148	functional foods	1133:1148	functional foods	1133:1148	These results may provide references for the exploitation of the SMS of L. sulphureus as a source of H-SMPS and E-SMPS, which in turn can be used as functional foods or natural drugs for the prevention of ALD and other liver diseases.
29430281	5	13	theme	E-SMPS	1096:1101	arg1	drugs					1161:1165	natural drugs	1153:1165	natural drugs	1153:1165	These results may provide references for the exploitation of the SMS of L. sulphureus as a source of H-SMPS and E-SMPS, which in turn can be used as functional foods or natural drugs for the prevention of ALD and other liver diseases.
29430281	5	13	theme	E-SMPS	1096:1101	arg1	references					1010:1019	references	1010:1019	references for the exploitation of the SMS of L. sulphureus	1010:1068	These results may provide references for the exploitation of the SMS of L. sulphureus as a source of H-SMPS and E-SMPS, which in turn can be used as functional foods or natural drugs for the prevention of ALD and other liver diseases.
29430281	5	13	theme	E-SMPS	1096:1101	arg1	source					1075:1080	a source	1073:1080	a source	1073:1080	These results may provide references for the exploitation of the SMS of L. sulphureus as a source of H-SMPS and E-SMPS, which in turn can be used as functional foods or natural drugs for the prevention of ALD and other liver diseases.
29430281	5	14	theme	ALD	1189:1191	arg1	prevention					1175:1184	the prevention	1171:1184	the prevention of ALD and other liver diseases	1171:1216	These results may provide references for the exploitation of the SMS of L. sulphureus as a source of H-SMPS and E-SMPS, which in turn can be used as functional foods or natural drugs for the prevention of ALD and other liver diseases.
29430281	0	15	dep	Substrates	83:92	arg1	sulphureus					106:115	Laetiporus sulphureus	95:115	Laetiporus sulphureus	95:115	Antioxidant and Hepatoprotective Activities of Polysaccharides from Spent Mushroom Substrates (Laetiporus sulphureus) in Acute Alcohol-Induced Mice.
29430281	0	16	theme	Hepatoprotective	16:31	arg1	Activities					33:42	Hepatoprotective Activities	16:42	Hepatoprotective Activities	16:42	Antioxidant and Hepatoprotective Activities of Polysaccharides from Spent Mushroom Substrates (Laetiporus sulphureus) in Acute Alcohol-Induced Mice.
29430281	0	17	theme	Alcohol-Induced	127:141	arg1	Mice					143:146	Acute Alcohol-Induced Mice	121:146	Acute Alcohol-Induced Mice	121:146	Antioxidant and Hepatoprotective Activities of Polysaccharides from Spent Mushroom Substrates (Laetiporus sulphureus) in Acute Alcohol-Induced Mice.
29430281	1	18	theme	spent	211:215	arg1	substrates					226:235	spent mushroom substrates	211:235	spent mushroom substrates	211:235	In order to contribute to the exploitation and utilization of spent mushroom substrates (SMS) of Laetiporus sulphureus, hot-water-extractable polysaccharides (H-SMPS) and enzymatic-extractable polysaccharides (E-SMPS) were successfully isolated from SMS of L. sulphureus.
29430281	0	19	theme	Polysaccharides	47:61	arg1	Antioxidant					0:10	Antioxidant	0:10	Antioxidant	0:10	Antioxidant and Hepatoprotective Activities of Polysaccharides from Spent Mushroom Substrates (Laetiporus sulphureus) in Acute Alcohol-Induced Mice.
29430281	0	19	theme	Polysaccharides	47:61	arg1	Activities					33:42	Hepatoprotective Activities	16:42	Hepatoprotective Activities	16:42	Antioxidant and Hepatoprotective Activities of Polysaccharides from Spent Mushroom Substrates (Laetiporus sulphureus) in Acute Alcohol-Induced Mice.
29430281	3	20	theme	liver	771:775	arg1	function					777:784	liver function	771:784	liver function	771:784	In vivo assays showed that the administration of H-SMPS and E-SMPS has potential hepatoprotective effects against alcohol-induced alcoholic liver disease (ALD), possibly brought about by improving liver function, increasing antioxidant status, and reducing lipid peroxidation.
29430281	2	21	theme	anion	558:562	arg1	radicals					564:571	superoxide anion radicals	547:571	superoxide anion radicals	547:571	Both H-SMPS and E-SMPS were found to have high reducing power and potential scavenging activities against hydroxyl, DPPH, and superoxide anion radicals.
29430281	1	22	theme	sulphureus	409:418	arg1	SMS					399:401	SMS	399:401	SMS of L. sulphureus	399:418	In order to contribute to the exploitation and utilization of spent mushroom substrates (SMS) of Laetiporus sulphureus, hot-water-extractable polysaccharides (H-SMPS) and enzymatic-extractable polysaccharides (E-SMPS) were successfully isolated from SMS of L. sulphureus.
29430281	5	23	theme	sulphureus	1059:1068	arg1	SMS					1049:1051	the SMS	1045:1051	the SMS of L. sulphureus	1045:1068	These results may provide references for the exploitation of the SMS of L. sulphureus as a source of H-SMPS and E-SMPS, which in turn can be used as functional foods or natural drugs for the prevention of ALD and other liver diseases.
29430281	1	24	theme	mushroom	217:224	arg1	substrates					226:235	spent mushroom substrates	211:235	spent mushroom substrates	211:235	In order to contribute to the exploitation and utilization of spent mushroom substrates (SMS) of Laetiporus sulphureus, hot-water-extractable polysaccharides (H-SMPS) and enzymatic-extractable polysaccharides (E-SMPS) were successfully isolated from SMS of L. sulphureus.
29430281	2	25	theme	superoxide	547:556	arg1	radicals					564:571	superoxide anion radicals	547:571	superoxide anion radicals	547:571	Both H-SMPS and E-SMPS were found to have high reducing power and potential scavenging activities against hydroxyl, DPPH, and superoxide anion radicals.
29430281	0	26	from	Substrates	83:92	arg1	Polysaccharides					47:61	Polysaccharides	47:61	Polysaccharides from Spent Mushroom Substrates (Laetiporus sulphureus)	47:116	Antioxidant and Hepatoprotective Activities of Polysaccharides from Spent Mushroom Substrates (Laetiporus sulphureus) in Acute Alcohol-Induced Mice.
29430281	0	26	from	Substrates	83:92	arg1	Antioxidant					0:10	Antioxidant	0:10	Antioxidant	0:10	Antioxidant and Hepatoprotective Activities of Polysaccharides from Spent Mushroom Substrates (Laetiporus sulphureus) in Acute Alcohol-Induced Mice.
29430281	0	26	from	Substrates	83:92	arg1	Activities					33:42	Hepatoprotective Activities	16:42	Hepatoprotective Activities	16:42	Antioxidant and Hepatoprotective Activities of Polysaccharides from Spent Mushroom Substrates (Laetiporus sulphureus) in Acute Alcohol-Induced Mice.
29430281	1	27	theme	substrates	226:235	arg1	utilization					196:206	utilization	196:206	utilization	196:206	In order to contribute to the exploitation and utilization of spent mushroom substrates (SMS) of Laetiporus sulphureus, hot-water-extractable polysaccharides (H-SMPS) and enzymatic-extractable polysaccharides (E-SMPS) were successfully isolated from SMS of L. sulphureus.
29430281	1	27	theme	substrates	226:235	arg1	exploitation					179:190	exploitation	179:190	exploitation	179:190	In order to contribute to the exploitation and utilization of spent mushroom substrates (SMS) of Laetiporus sulphureus, hot-water-extractable polysaccharides (H-SMPS) and enzymatic-extractable polysaccharides (E-SMPS) were successfully isolated from SMS of L. sulphureus.
29430281	1	27	theme	substrates	226:235	arg1	SMS					238:240	SMS	238:240	SMS	238:240	In order to contribute to the exploitation and utilization of spent mushroom substrates (SMS) of Laetiporus sulphureus, hot-water-extractable polysaccharides (H-SMPS) and enzymatic-extractable polysaccharides (E-SMPS) were successfully isolated from SMS of L. sulphureus.
29430281	0	28	theme	Spent	68:72	arg1	Substrates					83:92	Spent Mushroom Substrates	68:92	Spent Mushroom Substrates (Laetiporus sulphureus)	68:116	Antioxidant and Hepatoprotective Activities of Polysaccharides from Spent Mushroom Substrates (Laetiporus sulphureus) in Acute Alcohol-Induced Mice.
29430281	1	29	theme	enzymatic-extractable	320:340	arg1	E-SMPS					359:364	E-SMPS	359:364	E-SMPS	359:364	In order to contribute to the exploitation and utilization of spent mushroom substrates (SMS) of Laetiporus sulphureus, hot-water-extractable polysaccharides (H-SMPS) and enzymatic-extractable polysaccharides (E-SMPS) were successfully isolated from SMS of L. sulphureus.
29430281	1	29	theme	enzymatic-extractable	320:340	arg1	polysaccharides					342:356	enzymatic-extractable polysaccharides	320:356	enzymatic-extractable polysaccharides (E-SMPS)	320:365	In order to contribute to the exploitation and utilization of spent mushroom substrates (SMS) of Laetiporus sulphureus, hot-water-extractable polysaccharides (H-SMPS) and enzymatic-extractable polysaccharides (E-SMPS) were successfully isolated from SMS of L. sulphureus.
29430281	3	30	theme	hepatoprotective	655:670	arg1	effects					672:678	potential hepatoprotective effects	645:678	potential hepatoprotective effects	645:678	In vivo assays showed that the administration of H-SMPS and E-SMPS has potential hepatoprotective effects against alcohol-induced alcoholic liver disease (ALD), possibly brought about by improving liver function, increasing antioxidant status, and reducing lipid peroxidation.
29430281	4	31	theme	monosaccharide	864:877	arg1	analysis					891:898	monosaccharide composition analysis	864:898	monosaccharide composition analysis	864:898	Furthermore, monosaccharide composition analysis showed that fucose may play a vital role in guaranteeing stronger hepatoprotection.
29430281	2	32	theme	reducing	468:475	arg1	power					477:481	high reducing power	463:481	high reducing power	463:481	Both H-SMPS and E-SMPS were found to have high reducing power and potential scavenging activities against hydroxyl, DPPH, and superoxide anion radicals.
29430281	4	33	theme	composition	879:889	arg1	analysis					891:898	monosaccharide composition analysis	864:898	monosaccharide composition analysis	864:898	Furthermore, monosaccharide composition analysis showed that fucose may play a vital role in guaranteeing stronger hepatoprotection.
29430281	4	34	theme	vital	930:934	arg1	role					936:939	a vital role	928:939	a vital role	928:939	Furthermore, monosaccharide composition analysis showed that fucose may play a vital role in guaranteeing stronger hepatoprotection.
29430281	3	35	theme	liver	714:718	arg1	ALD					729:731	ALD	729:731	ALD	729:731	In vivo assays showed that the administration of H-SMPS and E-SMPS has potential hepatoprotective effects against alcohol-induced alcoholic liver disease (ALD), possibly brought about by improving liver function, increasing antioxidant status, and reducing lipid peroxidation.
29430281	3	35	theme	liver	714:718	arg1	disease					720:726	alcohol-induced alcoholic liver disease	688:726	alcohol-induced alcoholic liver disease (ALD)	688:732	In vivo assays showed that the administration of H-SMPS and E-SMPS has potential hepatoprotective effects against alcohol-induced alcoholic liver disease (ALD), possibly brought about by improving liver function, increasing antioxidant status, and reducing lipid peroxidation.
29430281	1	36	dep	exploitation	179:190	arg1	the					175:177	the	175:177	the	175:177	In order to contribute to the exploitation and utilization of spent mushroom substrates (SMS) of Laetiporus sulphureus, hot-water-extractable polysaccharides (H-SMPS) and enzymatic-extractable polysaccharides (E-SMPS) were successfully isolated from SMS of L. sulphureus.
29430281	3	37	theme	potential	645:653	arg1	effects					672:678	potential hepatoprotective effects	645:678	potential hepatoprotective effects	645:678	In vivo assays showed that the administration of H-SMPS and E-SMPS has potential hepatoprotective effects against alcohol-induced alcoholic liver disease (ALD), possibly brought about by improving liver function, increasing antioxidant status, and reducing lipid peroxidation.
29430281	3	38	theme	antioxidant	798:808	arg1	status					810:815	antioxidant status	798:815	antioxidant status	798:815	In vivo assays showed that the administration of H-SMPS and E-SMPS has potential hepatoprotective effects against alcohol-induced alcoholic liver disease (ALD), possibly brought about by improving liver function, increasing antioxidant status, and reducing lipid peroxidation.
29430281	5	39	theme	H-SMPS	1085:1090	arg1	foods					1144:1148	functional foods	1133:1148	functional foods	1133:1148	These results may provide references for the exploitation of the SMS of L. sulphureus as a source of H-SMPS and E-SMPS, which in turn can be used as functional foods or natural drugs for the prevention of ALD and other liver diseases.
29430281	5	39	theme	H-SMPS	1085:1090	arg1	drugs					1161:1165	natural drugs	1153:1165	natural drugs	1153:1165	These results may provide references for the exploitation of the SMS of L. sulphureus as a source of H-SMPS and E-SMPS, which in turn can be used as functional foods or natural drugs for the prevention of ALD and other liver diseases.
29430281	5	39	theme	H-SMPS	1085:1090	arg1	references					1010:1019	references	1010:1019	references for the exploitation of the SMS of L. sulphureus	1010:1068	These results may provide references for the exploitation of the SMS of L. sulphureus as a source of H-SMPS and E-SMPS, which in turn can be used as functional foods or natural drugs for the prevention of ALD and other liver diseases.
29430281	5	39	theme	H-SMPS	1085:1090	arg1	source					1075:1080	a source	1073:1080	a source	1073:1080	These results may provide references for the exploitation of the SMS of L. sulphureus as a source of H-SMPS and E-SMPS, which in turn can be used as functional foods or natural drugs for the prevention of ALD and other liver diseases.
29430281	3	40	theme	E-SMPS	634:639	arg1	administration					605:618	the administration	601:618	the administration of H-SMPS and E-SMPS	601:639	In vivo assays showed that the administration of H-SMPS and E-SMPS has potential hepatoprotective effects against alcohol-induced alcoholic liver disease (ALD), possibly brought about by improving liver function, increasing antioxidant status, and reducing lipid peroxidation.
29430281	3	41	theme	In	574:575	arg1	assays					582:587	In vivo assays	574:587	In vivo assays	574:587	In vivo assays showed that the administration of H-SMPS and E-SMPS has potential hepatoprotective effects against alcohol-induced alcoholic liver disease (ALD), possibly brought about by improving liver function, increasing antioxidant status, and reducing lipid peroxidation.
29430281	3	42	theme	lipid	831:835	arg1	peroxidation					837:848	lipid peroxidation	831:848	lipid peroxidation	831:848	In vivo assays showed that the administration of H-SMPS and E-SMPS has potential hepatoprotective effects against alcohol-induced alcoholic liver disease (ALD), possibly brought about by improving liver function, increasing antioxidant status, and reducing lipid peroxidation.
29430281	3	43	theme	alcohol-induced	688:702	arg1	ALD					729:731	ALD	729:731	ALD	729:731	In vivo assays showed that the administration of H-SMPS and E-SMPS has potential hepatoprotective effects against alcohol-induced alcoholic liver disease (ALD), possibly brought about by improving liver function, increasing antioxidant status, and reducing lipid peroxidation.
29430281	3	43	theme	alcohol-induced	688:702	arg1	disease					720:726	alcohol-induced alcoholic liver disease	688:726	alcohol-induced alcoholic liver disease (ALD)	688:732	In vivo assays showed that the administration of H-SMPS and E-SMPS has potential hepatoprotective effects against alcohol-induced alcoholic liver disease (ALD), possibly brought about by improving liver function, increasing antioxidant status, and reducing lipid peroxidation.
29430281	5	44	theme	SMS	1049:1051	arg1	exploitation					1029:1040	the exploitation	1025:1040	the exploitation of the SMS of L. sulphureus	1025:1068	These results may provide references for the exploitation of the SMS of L. sulphureus as a source of H-SMPS and E-SMPS, which in turn can be used as functional foods or natural drugs for the prevention of ALD and other liver diseases.
29430281	5	45	used	used	1125:1128	arg2	source					1075:1080	a source	1073:1080	a source	1073:1080	These results may provide references for the exploitation of the SMS of L. sulphureus as a source of H-SMPS and E-SMPS, which in turn can be used as functional foods or natural drugs for the prevention of ALD and other liver diseases.
29430281	5	45	used	used	1125:1128	arg2	drugs					1161:1165	natural drugs	1153:1165	natural drugs	1153:1165	These results may provide references for the exploitation of the SMS of L. sulphureus as a source of H-SMPS and E-SMPS, which in turn can be used as functional foods or natural drugs for the prevention of ALD and other liver diseases.
29430281	5	45	used	used	1125:1128	arg2	foods					1144:1148	functional foods	1133:1148	functional foods	1133:1148	These results may provide references for the exploitation of the SMS of L. sulphureus as a source of H-SMPS and E-SMPS, which in turn can be used as functional foods or natural drugs for the prevention of ALD and other liver diseases.
29430281	5	45	used	used	1125:1128	arg2	references					1010:1019	references	1010:1019	references for the exploitation of the SMS of L. sulphureus	1010:1068	These results may provide references for the exploitation of the SMS of L. sulphureus as a source of H-SMPS and E-SMPS, which in turn can be used as functional foods or natural drugs for the prevention of ALD and other liver diseases.
29430281	3	46	theme	alcoholic	704:712	arg1	ALD					729:731	ALD	729:731	ALD	729:731	In vivo assays showed that the administration of H-SMPS and E-SMPS has potential hepatoprotective effects against alcohol-induced alcoholic liver disease (ALD), possibly brought about by improving liver function, increasing antioxidant status, and reducing lipid peroxidation.
29430281	3	46	theme	alcoholic	704:712	arg1	disease					720:726	alcohol-induced alcoholic liver disease	688:726	alcohol-induced alcoholic liver disease (ALD)	688:732	In vivo assays showed that the administration of H-SMPS and E-SMPS has potential hepatoprotective effects against alcohol-induced alcoholic liver disease (ALD), possibly brought about by improving liver function, increasing antioxidant status, and reducing lipid peroxidation.
29430281	3	47	theme	H-SMPS	623:628	arg1	administration					605:618	the administration	601:618	the administration of H-SMPS and E-SMPS	601:639	In vivo assays showed that the administration of H-SMPS and E-SMPS has potential hepatoprotective effects against alcohol-induced alcoholic liver disease (ALD), possibly brought about by improving liver function, increasing antioxidant status, and reducing lipid peroxidation.
29430281	3	48	dep	In	574:575	arg1	vivo					577:580	vivo	577:580	vivo	577:580	In vivo assays showed that the administration of H-SMPS and E-SMPS has potential hepatoprotective effects against alcohol-induced alcoholic liver disease (ALD), possibly brought about by improving liver function, increasing antioxidant status, and reducing lipid peroxidation.
29430281	0	49	theme	Mushroom	74:81	arg1	Substrates					83:92	Spent Mushroom Substrates	68:92	Spent Mushroom Substrates (Laetiporus sulphureus)	68:116	Antioxidant and Hepatoprotective Activities of Polysaccharides from Spent Mushroom Substrates (Laetiporus sulphureus) in Acute Alcohol-Induced Mice.
29430281	0	50	from	Activities	33:42	arg1	Mice					143:146	Acute Alcohol-Induced Mice	121:146	Acute Alcohol-Induced Mice	121:146	Antioxidant and Hepatoprotective Activities of Polysaccharides from Spent Mushroom Substrates (Laetiporus sulphureus) in Acute Alcohol-Induced Mice.
29430281	0	50	from	Activities	33:42	arg1	Substrates					83:92	Spent Mushroom Substrates	68:92	Spent Mushroom Substrates (Laetiporus sulphureus)	68:116	Antioxidant and Hepatoprotective Activities of Polysaccharides from Spent Mushroom Substrates (Laetiporus sulphureus) in Acute Alcohol-Induced Mice.
29430281	1	51	attach	isolated	385:392	arg2	E-SMPS					359:364	E-SMPS	359:364	E-SMPS	359:364	In order to contribute to the exploitation and utilization of spent mushroom substrates (SMS) of Laetiporus sulphureus, hot-water-extractable polysaccharides (H-SMPS) and enzymatic-extractable polysaccharides (E-SMPS) were successfully isolated from SMS of L. sulphureus.
29430281	1	51	attach	isolated	385:392	arg2	polysaccharides					291:305	hot-water-extractable polysaccharides	269:305	hot-water-extractable polysaccharides (H-SMPS)	269:314	In order to contribute to the exploitation and utilization of spent mushroom substrates (SMS) of Laetiporus sulphureus, hot-water-extractable polysaccharides (H-SMPS) and enzymatic-extractable polysaccharides (E-SMPS) were successfully isolated from SMS of L. sulphureus.
29430281	1	51	attach	isolated	385:392	arg2	polysaccharides					342:356	enzymatic-extractable polysaccharides	320:356	enzymatic-extractable polysaccharides (E-SMPS)	320:365	In order to contribute to the exploitation and utilization of spent mushroom substrates (SMS) of Laetiporus sulphureus, hot-water-extractable polysaccharides (H-SMPS) and enzymatic-extractable polysaccharides (E-SMPS) were successfully isolated from SMS of L. sulphureus.
29430281	1	51	attach	isolated	385:392	arg1	SMS					399:401	SMS	399:401	SMS of L. sulphureus	399:418	In order to contribute to the exploitation and utilization of spent mushroom substrates (SMS) of Laetiporus sulphureus, hot-water-extractable polysaccharides (H-SMPS) and enzymatic-extractable polysaccharides (E-SMPS) were successfully isolated from SMS of L. sulphureus.
29430281	1	51	attach	isolated	385:392	arg2	H-SMPS					308:313	H-SMPS	308:313	H-SMPS	308:313	In order to contribute to the exploitation and utilization of spent mushroom substrates (SMS) of Laetiporus sulphureus, hot-water-extractable polysaccharides (H-SMPS) and enzymatic-extractable polysaccharides (E-SMPS) were successfully isolated from SMS of L. sulphureus.
29430281	5	52	theme	diseases	1209:1216	arg1	prevention					1175:1184	the prevention	1171:1184	the prevention of ALD and other liver diseases	1171:1216	These results may provide references for the exploitation of the SMS of L. sulphureus as a source of H-SMPS and E-SMPS, which in turn can be used as functional foods or natural drugs for the prevention of ALD and other liver diseases.
25340800	7	0	theme	wall	986:989	arg1	remodeling					1006:1015	cell wall polysaccharide remodeling	981:1015	cell wall polysaccharide remodeling	981:1015	KEY RESULTS The majority of the 22 up-regulated proteins were involved in the antioxidant defense pathway, cell wall polysaccharide remodeling, and cell metabolism process.
25340800	10	1	theme	putative	1339:1346	arg1	functions					1348:1356	their putative functions	1333:1356	their putative functions	1333:1356	CONCLUSIONS Based on the abundant changes in proteins and polysaccharides, and their putative functions, a possible protein interaction network can provide new insights into Cu stress response in root cell wall.
25340800	5	2	theme	6-plex	583:588	arg1	Tag					602:604	An recently developed 6-plex Tandem Mass Tag	561:604	An recently developed 6-plex Tandem Mass Tag	561:604	METHODS An recently developed 6-plex Tandem Mass Tag was used for relative and absolute quantitation methods to achieve a comprehensive understanding of Cu tolerance/detoxification molecular mechanisms in the cell wall.
25340800	8	3	theme	binding	1128:1134	arg1	sites					1136:1140	more binding sites	1123:1140	more binding sites for Cu ions	1123:1152	Changes in polysaccharide amount, composition, and distribution could offer more binding sites for Cu ions.
25340800	6	4	theme	cell	834:837	arg1	proteins					844:851	the Cu-responsive cell wall proteins	816:851	the Cu-responsive cell wall proteins	816:851	LC-MS/MS approach was performed to analyze the Cu-responsive cell wall proteins and polysaccharides.
25340800	2	5	from	effects	279:285	arg1	processes					324:332	the physiological and biochemical processes	290:332	the physiological and biochemical processes of plants	290:342	However, excess amounts of Cu are toxic and result in a wide range of harmful effects on the physiological and biochemical processes of plants.
25340800	10	6	theme	possible	1361:1368	arg1	network					1390:1396	a possible protein interaction network	1359:1396	a possible protein interaction network	1359:1396	CONCLUSIONS Based on the abundant changes in proteins and polysaccharides, and their putative functions, a possible protein interaction network can provide new insights into Cu stress response in root cell wall.
25340800	5	7	theme	Mass	597:600	arg1	Tag					602:604	An recently developed 6-plex Tandem Mass Tag	561:604	An recently developed 6-plex Tandem Mass Tag	561:604	METHODS An recently developed 6-plex Tandem Mass Tag was used for relative and absolute quantitation methods to achieve a comprehensive understanding of Cu tolerance/detoxification molecular mechanisms in the cell wall.
25340800	11	8	theme	functional	1492:1501	arg1	research					1503:1510	further functional research	1484:1510	further functional research	1484:1510	Cu can facilitate further functional research on target proteins associated with metal response in the cell wall.
25340800	2	9	theme	wide	257:260	arg1	range					262:266	a wide range	255:266	a wide range of harmful effects on the physiological and biochemical processes of plants	255:342	However, excess amounts of Cu are toxic and result in a wide range of harmful effects on the physiological and biochemical processes of plants.
25340800	7	10	theme	cell	1022:1025	arg1	metabolism					1027:1036	cell metabolism	1022:1036	cell metabolism	1022:1036	KEY RESULTS The majority of the 22 up-regulated proteins were involved in the antioxidant defense pathway, cell wall polysaccharide remodeling, and cell metabolism process.
25340800	1	11	theme	essential	165:173	arg1	micronutrient					175:187	an essential micronutrient	162:187	an essential micronutrient for plants	162:198	BACKGROUND AND AIMS Copper (Cu) is an essential micronutrient for plants.
25340800	1	11	theme	essential	165:173	arg1	Copper					147:152	BACKGROUND AND AIMS Copper	127:152	BACKGROUND AND AIMS Copper (Cu)	127:157	BACKGROUND AND AIMS Copper (Cu) is an essential micronutrient for plants.
25340800	0	12	from	wall	68:71	arg1	response					100:107	response	100:107	response to copper stress	100:124	New insights into regulation of proteome and polysaccharide in cell wall of Elsholtzia splendens in response to copper stress.
25340800	7	13	theme	defense	964:970	arg1	pathway					972:978	the antioxidant defense pathway	948:978	the antioxidant defense pathway	948:978	KEY RESULTS The majority of the 22 up-regulated proteins were involved in the antioxidant defense pathway, cell wall polysaccharide remodeling, and cell metabolism process.
25340800	7	14	theme	polysaccharide	991:1004	arg1	remodeling					1006:1015	cell wall polysaccharide remodeling	981:1015	cell wall polysaccharide remodeling	981:1015	KEY RESULTS The majority of the 22 up-regulated proteins were involved in the antioxidant defense pathway, cell wall polysaccharide remodeling, and cell metabolism process.
25340800	9	15	theme	signal	1207:1212	arg1	pathway					1214:1220	the signal pathway	1203:1220	the signal pathway	1203:1220	The 33 down-regulated proteins were involved in the signal pathway, energy, and protein synthesis.
25340800	5	16	from	wall	767:770	arg1	understanding					689:701	a comprehensive understanding	673:701	a comprehensive understanding of Cu tolerance/detoxification molecular mechanisms in the cell wall	673:770	METHODS An recently developed 6-plex Tandem Mass Tag was used for relative and absolute quantitation methods to achieve a comprehensive understanding of Cu tolerance/detoxification molecular mechanisms in the cell wall.
25340800	7	17	theme	cell	981:984	arg1	remodeling					1006:1015	cell wall polysaccharide remodeling	981:1015	cell wall polysaccharide remodeling	981:1015	KEY RESULTS The majority of the 22 up-regulated proteins were involved in the antioxidant defense pathway, cell wall polysaccharide remodeling, and cell metabolism process.
25340800	2	18	theme	excess	210:215	arg1	amounts					217:223	excess amounts	210:223	excess amounts of Cu	210:229	However, excess amounts of Cu are toxic and result in a wide range of harmful effects on the physiological and biochemical processes of plants.
25340800	2	18	theme	excess	210:215	arg1	Cu					228:229	Cu	228:229	Cu	228:229	However, excess amounts of Cu are toxic and result in a wide range of harmful effects on the physiological and biochemical processes of plants.
25340800	7	19	theme	proteins	922:929	arg1	majority					890:897	The majority	886:897	KEY RESULTS The majority of the 22 up-regulated proteins	874:929	KEY RESULTS The majority of the 22 up-regulated proteins were involved in the antioxidant defense pathway, cell wall polysaccharide remodeling, and cell metabolism process.
25340800	10	20	from	response	1438:1445	arg1	wall					1460:1463	root cell wall	1450:1463	root cell wall	1450:1463	CONCLUSIONS Based on the abundant changes in proteins and polysaccharides, and their putative functions, a possible protein interaction network can provide new insights into Cu stress response in root cell wall.
25340800	7	21	dep	RESULTS	878:884	arg1	majority					890:897	The majority	886:897	KEY RESULTS The majority of the 22 up-regulated proteins	874:929	KEY RESULTS The majority of the 22 up-regulated proteins were involved in the antioxidant defense pathway, cell wall polysaccharide remodeling, and cell metabolism process.
25340800	2	22	from	range	262:266	arg1	processes					324:332	the physiological and biochemical processes	290:332	the physiological and biochemical processes of plants	290:342	However, excess amounts of Cu are toxic and result in a wide range of harmful effects on the physiological and biochemical processes of plants.
25340800	10	23	theme	interaction	1378:1388	arg1	network					1390:1396	a possible protein interaction network	1359:1396	a possible protein interaction network	1359:1396	CONCLUSIONS Based on the abundant changes in proteins and polysaccharides, and their putative functions, a possible protein interaction network can provide new insights into Cu stress response in root cell wall.
25340800	0	24	theme	Elsholtzia	76:85	arg1	splendens					87:95	Elsholtzia splendens	76:95	Elsholtzia splendens	76:95	New insights into regulation of proteome and polysaccharide in cell wall of Elsholtzia splendens in response to copper stress.
25340800	5	25	from	mechanisms	744:753	arg1	wall					767:770	the cell wall	758:770	the cell wall	758:770	METHODS An recently developed 6-plex Tandem Mass Tag was used for relative and absolute quantitation methods to achieve a comprehensive understanding of Cu tolerance/detoxification molecular mechanisms in the cell wall.
25340800	10	26	theme	new	1410:1412	arg1	insights					1414:1421	new insights	1410:1421	new insights into Cu stress response in root cell wall	1410:1463	CONCLUSIONS Based on the abundant changes in proteins and polysaccharides, and their putative functions, a possible protein interaction network can provide new insights into Cu stress response in root cell wall.
25340800	7	27	dep	pathway	972:978	arg1	process					1038:1044	process	1038:1044	process	1038:1044	KEY RESULTS The majority of the 22 up-regulated proteins were involved in the antioxidant defense pathway, cell wall polysaccharide remodeling, and cell metabolism process.
25340800	3	28	theme	crucial	361:367	arg1	role					369:372	a crucial role	359:372	a crucial role	359:372	Cell wall has a crucial role in plant defense response to toxic metals.
25340800	0	29	theme	New	0:2	arg1	insights					4:11	New insights	0:11	New insights into regulation of proteome and polysaccharide in cell wall of Elsholtzia splendens in response to copper stress.	0:125	New insights into regulation of proteome and polysaccharide in cell wall of Elsholtzia splendens in response to copper stress.
25340800	7	30	theme	KEY	874:876	arg1	RESULTS					878:884	KEY RESULTS	874:884	KEY RESULTS The majority of the 22 up-regulated proteins	874:929	KEY RESULTS The majority of the 22 up-regulated proteins were involved in the antioxidant defense pathway, cell wall polysaccharide remodeling, and cell metabolism process.
25340800	3	31	contain	has	355:357	arg1	wall					350:353	Cell wall	345:353	Cell wall	345:353	Cell wall has a crucial role in plant defense response to toxic metals.
25340800	3	31	contain	has	355:357	arg2	role					369:372	a crucial role	359:372	a crucial role	359:372	Cell wall has a crucial role in plant defense response to toxic metals.
25340800	5	32	theme	cell	762:765	arg1	wall					767:770	the cell wall	758:770	the cell wall	758:770	METHODS An recently developed 6-plex Tandem Mass Tag was used for relative and absolute quantitation methods to achieve a comprehensive understanding of Cu tolerance/detoxification molecular mechanisms in the cell wall.
25340800	10	33	theme	stress	1431:1436	arg1	response					1438:1445	Cu stress response	1428:1445	Cu stress response in root cell wall	1428:1463	CONCLUSIONS Based on the abundant changes in proteins and polysaccharides, and their putative functions, a possible protein interaction network can provide new insights into Cu stress response in root cell wall.
25340800	3	34	theme	defense	383:389	arg1	response					391:398	plant defense response	377:398	plant defense response to toxic metals	377:414	Cell wall has a crucial role in plant defense response to toxic metals.
25340800	5	35	theme	Cu	706:707	arg1	mechanisms					744:753	Cu tolerance/detoxification molecular mechanisms	706:753	Cu tolerance/detoxification molecular mechanisms in the cell wall	706:770	METHODS An recently developed 6-plex Tandem Mass Tag was used for relative and absolute quantitation methods to achieve a comprehensive understanding of Cu tolerance/detoxification molecular mechanisms in the cell wall.
25340800	3	36	theme	Cell	345:348	arg1	wall					350:353	Cell wall	345:353	Cell wall	345:353	Cell wall has a crucial role in plant defense response to toxic metals.
25340800	4	37	theme	cell	441:444	arg1	response					451:458	cell wall response	441:458	cell wall response to Cu	441:464	To date, the process of cell wall response to Cu and the detoxification mechanism have not been well documented at the proteomic level.
25340800	10	38	theme	abundant	1279:1286	arg1	changes					1288:1294	the abundant changes	1275:1294	the abundant changes in proteins and polysaccharides, and their putative functions, a possible protein interaction network	1275:1396	CONCLUSIONS Based on the abundant changes in proteins and polysaccharides, and their putative functions, a possible protein interaction network can provide new insights into Cu stress response in root cell wall.
25340800	5	39	theme	molecular	734:742	arg1	mechanisms					744:753	Cu tolerance/detoxification molecular mechanisms	706:753	Cu tolerance/detoxification molecular mechanisms in the cell wall	706:770	METHODS An recently developed 6-plex Tandem Mass Tag was used for relative and absolute quantitation methods to achieve a comprehensive understanding of Cu tolerance/detoxification molecular mechanisms in the cell wall.
25340800	4	40	theme	response	451:458	arg1	mechanism					489:497	the detoxification mechanism	470:497	the detoxification mechanism	470:497	To date, the process of cell wall response to Cu and the detoxification mechanism have not been well documented at the proteomic level.
25340800	4	40	theme	response	451:458	arg1	process					430:436	the process	426:436	the process of cell wall response to Cu	426:464	To date, the process of cell wall response to Cu and the detoxification mechanism have not been well documented at the proteomic level.
25340800	10	41	theme	cell	1455:1458	arg1	wall					1460:1463	root cell wall	1450:1463	root cell wall	1450:1463	CONCLUSIONS Based on the abundant changes in proteins and polysaccharides, and their putative functions, a possible protein interaction network can provide new insights into Cu stress response in root cell wall.
25340800	2	42	theme	harmful	271:277	arg1	effects					279:285	harmful effects	271:285	harmful effects on the physiological and biochemical processes of plants	271:342	However, excess amounts of Cu are toxic and result in a wide range of harmful effects on the physiological and biochemical processes of plants.
25340800	11	43	theme	cell	1569:1572	arg1	wall					1574:1577	the cell wall	1565:1577	the cell wall	1565:1577	Cu can facilitate further functional research on target proteins associated with metal response in the cell wall.
25340800	2	44	theme	biochemical	312:322	arg1	processes					324:332	the physiological and biochemical processes	290:332	the physiological and biochemical processes of plants	290:342	However, excess amounts of Cu are toxic and result in a wide range of harmful effects on the physiological and biochemical processes of plants.
25340800	0	45	theme	polysaccharide	45:58	arg1	regulation					18:27	regulation	18:27	regulation of proteome and polysaccharide in cell wall of Elsholtzia splendens in response to copper stress	18:124	New insights into regulation of proteome and polysaccharide in cell wall of Elsholtzia splendens in response to copper stress.
25340800	2	46	theme	physiological	294:306	arg1	processes					324:332	the physiological and biochemical processes	290:332	the physiological and biochemical processes of plants	290:342	However, excess amounts of Cu are toxic and result in a wide range of harmful effects on the physiological and biochemical processes of plants.
25340800	5	47	theme	developed	573:581	arg1	Tag					602:604	An recently developed 6-plex Tandem Mass Tag	561:604	An recently developed 6-plex Tandem Mass Tag	561:604	METHODS An recently developed 6-plex Tandem Mass Tag was used for relative and absolute quantitation methods to achieve a comprehensive understanding of Cu tolerance/detoxification molecular mechanisms in the cell wall.
25340800	11	48	theme	further	1484:1490	arg1	research					1503:1510	further functional research	1484:1510	further functional research	1484:1510	Cu can facilitate further functional research on target proteins associated with metal response in the cell wall.
25340800	6	49	theme	wall	839:842	arg1	proteins					844:851	the Cu-responsive cell wall proteins	816:851	the Cu-responsive cell wall proteins	816:851	LC-MS/MS approach was performed to analyze the Cu-responsive cell wall proteins and polysaccharides.
25340800	11	50	theme	metal	1547:1551	arg1	response					1553:1560	metal response	1547:1560	metal response in the cell wall	1547:1577	Cu can facilitate further functional research on target proteins associated with metal response in the cell wall.
25340800	11	51	from	response	1553:1560	arg1	wall					1574:1577	the cell wall	1565:1577	the cell wall	1565:1577	Cu can facilitate further functional research on target proteins associated with metal response in the cell wall.
25340800	8	52	theme	more	1123:1126	arg1	sites					1136:1140	more binding sites	1123:1140	more binding sites for Cu ions	1123:1152	Changes in polysaccharide amount, composition, and distribution could offer more binding sites for Cu ions.
25340800	6	53	theme	Cu-responsive	820:832	arg1	proteins					844:851	the Cu-responsive cell wall proteins	816:851	the Cu-responsive cell wall proteins	816:851	LC-MS/MS approach was performed to analyze the Cu-responsive cell wall proteins and polysaccharides.
25340800	11	54	theme	target	1515:1520	arg1	proteins					1522:1529	target proteins	1515:1529	target proteins associated with metal response in the cell wall	1515:1577	Cu can facilitate further functional research on target proteins associated with metal response in the cell wall.
25340800	0	55	theme	copper	112:117	arg1	stress					119:124	copper stress	112:124	copper stress	112:124	New insights into regulation of proteome and polysaccharide in cell wall of Elsholtzia splendens in response to copper stress.
25340800	9	56	theme	down-regulated	1162:1175	arg1	proteins					1177:1184	The 33 down-regulated proteins	1155:1184	The 33 down-regulated proteins	1155:1184	The 33 down-regulated proteins were involved in the signal pathway, energy, and protein synthesis.
25340800	8	57	theme	Cu	1146:1147	arg1	ions					1149:1152	Cu ions	1146:1152	Cu ions	1146:1152	Changes in polysaccharide amount, composition, and distribution could offer more binding sites for Cu ions.
25340800	4	58	theme	proteomic	536:544	arg1	level					546:550	the proteomic level	532:550	the proteomic level	532:550	To date, the process of cell wall response to Cu and the detoxification mechanism have not been well documented at the proteomic level.
25340800	5	59	theme	relative	619:626	arg1	methods					654:660	relative and absolute quantitation methods	619:660	relative and absolute quantitation methods	619:660	METHODS An recently developed 6-plex Tandem Mass Tag was used for relative and absolute quantitation methods to achieve a comprehensive understanding of Cu tolerance/detoxification molecular mechanisms in the cell wall.
25340800	5	60	theme	absolute	632:639	arg1	methods					654:660	relative and absolute quantitation methods	619:660	relative and absolute quantitation methods	619:660	METHODS An recently developed 6-plex Tandem Mass Tag was used for relative and absolute quantitation methods to achieve a comprehensive understanding of Cu tolerance/detoxification molecular mechanisms in the cell wall.
25340800	5	61	theme	Tandem	590:595	arg1	Tag					602:604	An recently developed 6-plex Tandem Mass Tag	561:604	An recently developed 6-plex Tandem Mass Tag	561:604	METHODS An recently developed 6-plex Tandem Mass Tag was used for relative and absolute quantitation methods to achieve a comprehensive understanding of Cu tolerance/detoxification molecular mechanisms in the cell wall.
25340800	5	62	used	used	610:613	arg2	Tag					602:604	An recently developed 6-plex Tandem Mass Tag	561:604	An recently developed 6-plex Tandem Mass Tag	561:604	METHODS An recently developed 6-plex Tandem Mass Tag was used for relative and absolute quantitation methods to achieve a comprehensive understanding of Cu tolerance/detoxification molecular mechanisms in the cell wall.
25340800	5	63	from	understanding	689:701	arg1	wall					767:770	the cell wall	758:770	the cell wall	758:770	METHODS An recently developed 6-plex Tandem Mass Tag was used for relative and absolute quantitation methods to achieve a comprehensive understanding of Cu tolerance/detoxification molecular mechanisms in the cell wall.
25340800	2	64	theme	Cu	228:229	arg1	amounts					217:223	excess amounts	210:223	excess amounts of Cu	210:229	However, excess amounts of Cu are toxic and result in a wide range of harmful effects on the physiological and biochemical processes of plants.
25340800	2	64	theme	Cu	228:229	arg1	Cu					228:229	Cu	228:229	Cu	228:229	However, excess amounts of Cu are toxic and result in a wide range of harmful effects on the physiological and biochemical processes of plants.
25340800	7	65	theme	antioxidant	952:962	arg1	pathway					972:978	the antioxidant defense pathway	948:978	the antioxidant defense pathway	948:978	KEY RESULTS The majority of the 22 up-regulated proteins were involved in the antioxidant defense pathway, cell wall polysaccharide remodeling, and cell metabolism process.
25340800	8	66	from	Changes	1047:1053	arg1	composition					1081:1091	composition	1081:1091	composition	1081:1091	Changes in polysaccharide amount, composition, and distribution could offer more binding sites for Cu ions.
25340800	8	66	from	Changes	1047:1053	arg1	distribution					1098:1109	distribution	1098:1109	distribution	1098:1109	Changes in polysaccharide amount, composition, and distribution could offer more binding sites for Cu ions.
25340800	8	66	from	Changes	1047:1053	arg1	amount					1073:1078	polysaccharide amount	1058:1078	polysaccharide amount	1058:1078	Changes in polysaccharide amount, composition, and distribution could offer more binding sites for Cu ions.
25340800	0	67	theme	cell	63:66	arg1	wall					68:71	cell wall	63:71	cell wall of Elsholtzia splendens in response to copper stress	63:124	New insights into regulation of proteome and polysaccharide in cell wall of Elsholtzia splendens in response to copper stress.
25340800	1	68	theme	BACKGROUND	127:136	arg1	micronutrient					175:187	an essential micronutrient	162:187	an essential micronutrient for plants	162:198	BACKGROUND AND AIMS Copper (Cu) is an essential micronutrient for plants.
25340800	1	68	theme	BACKGROUND	127:136	arg1	Cu					155:156	Cu	155:156	Cu	155:156	BACKGROUND AND AIMS Copper (Cu) is an essential micronutrient for plants.
25340800	1	68	theme	BACKGROUND	127:136	arg1	Copper					147:152	BACKGROUND AND AIMS Copper	127:152	BACKGROUND AND AIMS Copper (Cu)	127:157	BACKGROUND AND AIMS Copper (Cu) is an essential micronutrient for plants.
25340800	0	69	from	regulation	18:27	arg1	wall					68:71	cell wall	63:71	cell wall of Elsholtzia splendens in response to copper stress	63:124	New insights into regulation of proteome and polysaccharide in cell wall of Elsholtzia splendens in response to copper stress.
25340800	5	70	theme	quantitation	641:652	arg1	methods					654:660	relative and absolute quantitation methods	619:660	relative and absolute quantitation methods	619:660	METHODS An recently developed 6-plex Tandem Mass Tag was used for relative and absolute quantitation methods to achieve a comprehensive understanding of Cu tolerance/detoxification molecular mechanisms in the cell wall.
25340800	5	71	theme	comprehensive	675:687	arg1	understanding					689:701	a comprehensive understanding	673:701	a comprehensive understanding of Cu tolerance/detoxification molecular mechanisms in the cell wall	673:770	METHODS An recently developed 6-plex Tandem Mass Tag was used for relative and absolute quantitation methods to achieve a comprehensive understanding of Cu tolerance/detoxification molecular mechanisms in the cell wall.
25340800	1	72	theme	AIMS	142:145	arg1	micronutrient					175:187	an essential micronutrient	162:187	an essential micronutrient for plants	162:198	BACKGROUND AND AIMS Copper (Cu) is an essential micronutrient for plants.
25340800	1	72	theme	AIMS	142:145	arg1	Cu					155:156	Cu	155:156	Cu	155:156	BACKGROUND AND AIMS Copper (Cu) is an essential micronutrient for plants.
25340800	1	72	theme	AIMS	142:145	arg1	Copper					147:152	BACKGROUND AND AIMS Copper	127:152	BACKGROUND AND AIMS Copper (Cu)	127:157	BACKGROUND AND AIMS Copper (Cu) is an essential micronutrient for plants.
25340800	0	73	theme	splendens	87:95	arg1	wall					68:71	cell wall	63:71	cell wall of Elsholtzia splendens in response to copper stress	63:124	New insights into regulation of proteome and polysaccharide in cell wall of Elsholtzia splendens in response to copper stress.
25340800	9	74	theme	protein	1235:1241	arg1	synthesis					1243:1251	protein synthesis	1235:1251	protein synthesis	1235:1251	The 33 down-regulated proteins were involved in the signal pathway, energy, and protein synthesis.
25340800	7	75	theme	up-regulated	909:920	arg1	proteins					922:929	the 22 up-regulated proteins	902:929	the 22 up-regulated proteins	902:929	KEY RESULTS The majority of the 22 up-regulated proteins were involved in the antioxidant defense pathway, cell wall polysaccharide remodeling, and cell metabolism process.
25340800	10	76	theme	protein	1370:1376	arg1	network					1390:1396	a possible protein interaction network	1359:1396	a possible protein interaction network	1359:1396	CONCLUSIONS Based on the abundant changes in proteins and polysaccharides, and their putative functions, a possible protein interaction network can provide new insights into Cu stress response in root cell wall.
25340800	2	77	theme	plants	337:342	arg1	processes					324:332	the physiological and biochemical processes	290:332	the physiological and biochemical processes of plants	290:342	However, excess amounts of Cu are toxic and result in a wide range of harmful effects on the physiological and biochemical processes of plants.
25340800	5	78	theme	mechanisms	744:753	arg1	understanding					689:701	a comprehensive understanding	673:701	a comprehensive understanding of Cu tolerance/detoxification molecular mechanisms in the cell wall	673:770	METHODS An recently developed 6-plex Tandem Mass Tag was used for relative and absolute quantitation methods to achieve a comprehensive understanding of Cu tolerance/detoxification molecular mechanisms in the cell wall.
25340800	10	79	from	changes	1288:1294	arg1	network					1390:1396	a possible protein interaction network	1359:1396	a possible protein interaction network	1359:1396	CONCLUSIONS Based on the abundant changes in proteins and polysaccharides, and their putative functions, a possible protein interaction network can provide new insights into Cu stress response in root cell wall.
25340800	10	79	from	changes	1288:1294	arg1	proteins					1299:1306	proteins	1299:1306	proteins	1299:1306	CONCLUSIONS Based on the abundant changes in proteins and polysaccharides, and their putative functions, a possible protein interaction network can provide new insights into Cu stress response in root cell wall.
25340800	10	79	from	changes	1288:1294	arg1	functions					1348:1356	their putative functions	1333:1356	their putative functions	1333:1356	CONCLUSIONS Based on the abundant changes in proteins and polysaccharides, and their putative functions, a possible protein interaction network can provide new insights into Cu stress response in root cell wall.
25340800	10	79	from	changes	1288:1294	arg1	polysaccharides					1312:1326	polysaccharides	1312:1326	polysaccharides	1312:1326	CONCLUSIONS Based on the abundant changes in proteins and polysaccharides, and their putative functions, a possible protein interaction network can provide new insights into Cu stress response in root cell wall.
25340800	4	80	theme	detoxification	474:487	arg1	mechanism					489:497	the detoxification mechanism	470:497	the detoxification mechanism	470:497	To date, the process of cell wall response to Cu and the detoxification mechanism have not been well documented at the proteomic level.
25340800	3	81	theme	toxic	403:407	arg1	metals					409:414	toxic metals	403:414	toxic metals	403:414	Cell wall has a crucial role in plant defense response to toxic metals.
25340800	0	82	theme	proteome	32:39	arg1	regulation					18:27	regulation	18:27	regulation of proteome and polysaccharide in cell wall of Elsholtzia splendens in response to copper stress	18:124	New insights into regulation of proteome and polysaccharide in cell wall of Elsholtzia splendens in response to copper stress.
25340800	5	83	dep	METHODS	553:559	arg1	used					610:613	used	610:613	was used for relative and absolute quantitation methods to achieve a comprehensive understanding of Cu tolerance/detoxification molecular mechanisms in the cell wall	606:770	METHODS An recently developed 6-plex Tandem Mass Tag was used for relative and absolute quantitation methods to achieve a comprehensive understanding of Cu tolerance/detoxification molecular mechanisms in the cell wall.
25340800	4	84	theme	wall	446:449	arg1	response					451:458	cell wall response	441:458	cell wall response to Cu	441:464	To date, the process of cell wall response to Cu and the detoxification mechanism have not been well documented at the proteomic level.
25340800	10	85	theme	Cu	1428:1429	arg1	response					1438:1445	Cu stress response	1428:1445	Cu stress response in root cell wall	1428:1463	CONCLUSIONS Based on the abundant changes in proteins and polysaccharides, and their putative functions, a possible protein interaction network can provide new insights into Cu stress response in root cell wall.
25340800	5	86	theme	tolerance/detoxification	709:732	arg1	mechanisms					744:753	Cu tolerance/detoxification molecular mechanisms	706:753	Cu tolerance/detoxification molecular mechanisms in the cell wall	706:770	METHODS An recently developed 6-plex Tandem Mass Tag was used for relative and absolute quantitation methods to achieve a comprehensive understanding of Cu tolerance/detoxification molecular mechanisms in the cell wall.
25340800	8	87	theme	polysaccharide	1058:1071	arg1	amount					1073:1078	polysaccharide amount	1058:1078	polysaccharide amount	1058:1078	Changes in polysaccharide amount, composition, and distribution could offer more binding sites for Cu ions.
25340800	2	88	from	processes	324:332	arg1	range					262:266	a wide range	255:266	a wide range of harmful effects on the physiological and biochemical processes of plants	255:342	However, excess amounts of Cu are toxic and result in a wide range of harmful effects on the physiological and biochemical processes of plants.
25340800	2	89	theme	effects	279:285	arg1	range					262:266	a wide range	255:266	a wide range of harmful effects on the physiological and biochemical processes of plants	255:342	However, excess amounts of Cu are toxic and result in a wide range of harmful effects on the physiological and biochemical processes of plants.
25340800	6	90	theme	LC-MS/MS	773:780	arg1	approach					782:789	LC-MS/MS approach	773:789	LC-MS/MS approach	773:789	LC-MS/MS approach was performed to analyze the Cu-responsive cell wall proteins and polysaccharides.
25340800	10	91	theme	root	1450:1453	arg1	wall					1460:1463	root cell wall	1450:1463	root cell wall	1450:1463	CONCLUSIONS Based on the abundant changes in proteins and polysaccharides, and their putative functions, a possible protein interaction network can provide new insights into Cu stress response in root cell wall.
25340800	3	92	theme	plant	377:381	arg1	response					391:398	plant defense response	377:398	plant defense response to toxic metals	377:414	Cell wall has a crucial role in plant defense response to toxic metals.
27686723	1	0	from	delignification	328:342	arg1	terms					347:351	terms	347:351	terms of the final compositions of cellulose, hemicellulose and lignin	347:416	Experiments have been performed for pretreatment of sorghum, wheat straw and bamboo through high temperature alkali pretreatment with different alkaline loading and temperatures, and the data on extent of delignification in terms of the final compositions of cellulose, hemicellulose and lignin have been generated.
27686723	0	1	with	saccharification	30:45	arg1	feedstocks					62:71	different feedstocks	52:71	different feedstocks	52:71	Modelling of pretreatment and saccharification with different feedstocks and kinetic modeling of sorghum saccharification.
27686723	1	2	theme	sorghum	175:181	arg1	straw					190:194	sorghum, wheat straw	175:194	straw	190:194	Experiments have been performed for pretreatment of sorghum, wheat straw and bamboo through high temperature alkali pretreatment with different alkaline loading and temperatures, and the data on extent of delignification in terms of the final compositions of cellulose, hemicellulose and lignin have been generated.
27686723	4	3	theme	specific	883:890	arg1	biomass					892:898	specific biomass	883:898	specific biomass respectively with alkaline pretreatment	883:938	This data is employed to develop a generalized multi-feedstock and individual feedstock based models which can be used to determine the extent of delignification and cellulose conversion for any and specific biomass respectively with alkaline pretreatment and similar enzyme conditions as considered in the present study.
27686723	4	4	theme	biomass	892:898	arg1	conditions					959:968	any and specific biomass respectively with alkaline pretreatment and similar enzyme conditions	875:968	any and specific biomass respectively with alkaline pretreatment and similar enzyme conditions as considered in the present study	875:1003	This data is employed to develop a generalized multi-feedstock and individual feedstock based models which can be used to determine the extent of delignification and cellulose conversion for any and specific biomass respectively with alkaline pretreatment and similar enzyme conditions as considered in the present study.
27686723	0	5	theme	saccharification	105:120	arg1	modeling					85:92	kinetic modeling	77:92	kinetic modeling of sorghum saccharification	77:120	Modelling of pretreatment and saccharification with different feedstocks and kinetic modeling of sorghum saccharification.
27686723	0	5	theme	saccharification	105:120	arg1	Modelling					0:8	Modelling	0:8	Modelling of pretreatment and saccharification with different feedstocks	0:71	Modelling of pretreatment and saccharification with different feedstocks and kinetic modeling of sorghum saccharification.
27686723	1	6	theme	final	360:364	arg1	compositions					366:377	the final compositions	356:377	the final compositions of cellulose, hemicellulose and lignin	356:416	Experiments have been performed for pretreatment of sorghum, wheat straw and bamboo through high temperature alkali pretreatment with different alkaline loading and temperatures, and the data on extent of delignification in terms of the final compositions of cellulose, hemicellulose and lignin have been generated.
27686723	1	7	from	extent	318:323	arg1	terms					347:351	terms	347:351	terms of the final compositions of cellulose, hemicellulose and lignin	347:416	Experiments have been performed for pretreatment of sorghum, wheat straw and bamboo through high temperature alkali pretreatment with different alkaline loading and temperatures, and the data on extent of delignification in terms of the final compositions of cellulose, hemicellulose and lignin have been generated.
27686723	4	8	theme	cellulose	850:858	arg1	conversion					860:869	cellulose conversion	850:869	cellulose conversion	850:869	This data is employed to develop a generalized multi-feedstock and individual feedstock based models which can be used to determine the extent of delignification and cellulose conversion for any and specific biomass respectively with alkaline pretreatment and similar enzyme conditions as considered in the present study.
27686723	0	9	theme	sorghum	97:103	arg1	saccharification					105:120	sorghum saccharification	97:120	sorghum saccharification	97:120	Modelling of pretreatment and saccharification with different feedstocks and kinetic modeling of sorghum saccharification.
27686723	1	10	theme	compositions	366:377	arg1	terms					347:351	terms	347:351	terms of the final compositions of cellulose, hemicellulose and lignin	347:416	Experiments have been performed for pretreatment of sorghum, wheat straw and bamboo through high temperature alkali pretreatment with different alkaline loading and temperatures, and the data on extent of delignification in terms of the final compositions of cellulose, hemicellulose and lignin have been generated.
27686723	1	11	from	data	310:313	arg1	extent					318:323	extent	318:323	extent of delignification in terms of the final compositions of cellulose, hemicellulose and lignin	318:416	Experiments have been performed for pretreatment of sorghum, wheat straw and bamboo through high temperature alkali pretreatment with different alkaline loading and temperatures, and the data on extent of delignification in terms of the final compositions of cellulose, hemicellulose and lignin have been generated.
27686723	4	12	with	biomass	892:898	arg1	pretreatment					927:938	alkaline pretreatment	918:938	alkaline pretreatment	918:938	This data is employed to develop a generalized multi-feedstock and individual feedstock based models which can be used to determine the extent of delignification and cellulose conversion for any and specific biomass respectively with alkaline pretreatment and similar enzyme conditions as considered in the present study.
27686723	4	13	theme	present	991:997	arg1	study					999:1003	the present study	987:1003	the present study	987:1003	This data is employed to develop a generalized multi-feedstock and individual feedstock based models which can be used to determine the extent of delignification and cellulose conversion for any and specific biomass respectively with alkaline pretreatment and similar enzyme conditions as considered in the present study.
27686723	1	14	theme	cellulose	382:390	arg1	compositions					366:377	the final compositions	356:377	the final compositions of cellulose, hemicellulose and lignin	356:416	Experiments have been performed for pretreatment of sorghum, wheat straw and bamboo through high temperature alkali pretreatment with different alkaline loading and temperatures, and the data on extent of delignification in terms of the final compositions of cellulose, hemicellulose and lignin have been generated.
27686723	4	15	theme	conversion	860:869	arg1	extent					820:825	the extent	816:825	the extent of delignification and cellulose conversion for any and specific biomass respectively with alkaline pretreatment and similar enzyme conditions as considered in the present study	816:1003	This data is employed to develop a generalized multi-feedstock and individual feedstock based models which can be used to determine the extent of delignification and cellulose conversion for any and specific biomass respectively with alkaline pretreatment and similar enzyme conditions as considered in the present study.
27686723	1	16	theme	straw	190:194	arg1	pretreatment					159:170	pretreatment	159:170	pretreatment of sorghum, wheat straw	159:194	Experiments have been performed for pretreatment of sorghum, wheat straw and bamboo through high temperature alkali pretreatment with different alkaline loading and temperatures, and the data on extent of delignification in terms of the final compositions of cellulose, hemicellulose and lignin have been generated.
27686723	4	17	theme	feedstock	762:770	arg1	models					778:783	a generalized multi-feedstock and individual feedstock based models	717:783	a generalized multi-feedstock and individual feedstock based models which can be used to determine the extent of delignification and cellulose conversion for any and specific biomass respectively with alkaline pretreatment and similar enzyme conditions as considered in the present study	717:1003	This data is employed to develop a generalized multi-feedstock and individual feedstock based models which can be used to determine the extent of delignification and cellulose conversion for any and specific biomass respectively with alkaline pretreatment and similar enzyme conditions as considered in the present study.
27686723	4	18	with	enzyme	952:957	arg1	pretreatment					927:938	alkaline pretreatment	918:938	alkaline pretreatment	918:938	This data is employed to develop a generalized multi-feedstock and individual feedstock based models which can be used to determine the extent of delignification and cellulose conversion for any and specific biomass respectively with alkaline pretreatment and similar enzyme conditions as considered in the present study.
27686723	4	19	theme	individual	751:760	arg1	models					778:783	a generalized multi-feedstock and individual feedstock based models	717:783	a generalized multi-feedstock and individual feedstock based models which can be used to determine the extent of delignification and cellulose conversion for any and specific biomass respectively with alkaline pretreatment and similar enzyme conditions as considered in the present study	717:1003	This data is employed to develop a generalized multi-feedstock and individual feedstock based models which can be used to determine the extent of delignification and cellulose conversion for any and specific biomass respectively with alkaline pretreatment and similar enzyme conditions as considered in the present study.
27686723	2	20	theme	enzymatic	448:456	arg1	saccharification					458:473	enzymatic saccharification	448:473	enzymatic saccharification	448:473	Further, enzymatic saccharification has been carried out in all the cases to find the extent of conversion possible after 72h.
27686723	3	21	theme	operating	590:598	arg1	parameters					600:609	different operating parameters	580:609	different operating parameters	580:609	The effect of different operating parameters on the extent of delignification and cellulose conversion are evaluated.
27686723	4	22	theme	delignification	830:844	arg1	extent					820:825	the extent	816:825	the extent of delignification and cellulose conversion for any and specific biomass respectively with alkaline pretreatment and similar enzyme conditions as considered in the present study	816:1003	This data is employed to develop a generalized multi-feedstock and individual feedstock based models which can be used to determine the extent of delignification and cellulose conversion for any and specific biomass respectively with alkaline pretreatment and similar enzyme conditions as considered in the present study.
27686723	0	23	theme	pretreatment	13:24	arg1	modeling					85:92	kinetic modeling	77:92	kinetic modeling of sorghum saccharification	77:120	Modelling of pretreatment and saccharification with different feedstocks and kinetic modeling of sorghum saccharification.
27686723	0	23	theme	pretreatment	13:24	arg1	Modelling					0:8	Modelling	0:8	Modelling of pretreatment and saccharification with different feedstocks	0:71	Modelling of pretreatment and saccharification with different feedstocks and kinetic modeling of sorghum saccharification.
27686723	4	24	theme	enzyme	952:957	arg1	conditions					959:968	any and specific biomass respectively with alkaline pretreatment and similar enzyme conditions	875:968	any and specific biomass respectively with alkaline pretreatment and similar enzyme conditions as considered in the present study	875:1003	This data is employed to develop a generalized multi-feedstock and individual feedstock based models which can be used to determine the extent of delignification and cellulose conversion for any and specific biomass respectively with alkaline pretreatment and similar enzyme conditions as considered in the present study.
27686723	1	25	theme	hemicellulose	393:405	arg1	compositions					366:377	the final compositions	356:377	the final compositions of cellulose, hemicellulose and lignin	356:416	Experiments have been performed for pretreatment of sorghum, wheat straw and bamboo through high temperature alkali pretreatment with different alkaline loading and temperatures, and the data on extent of delignification in terms of the final compositions of cellulose, hemicellulose and lignin have been generated.
27686723	4	26	theme	based	772:776	arg1	models					778:783	a generalized multi-feedstock and individual feedstock based models	717:783	a generalized multi-feedstock and individual feedstock based models which can be used to determine the extent of delignification and cellulose conversion for any and specific biomass respectively with alkaline pretreatment and similar enzyme conditions as considered in the present study	717:1003	This data is employed to develop a generalized multi-feedstock and individual feedstock based models which can be used to determine the extent of delignification and cellulose conversion for any and specific biomass respectively with alkaline pretreatment and similar enzyme conditions as considered in the present study.
27686723	1	27	theme	high	215:218	arg1	pretreatment					239:250	high temperature alkali pretreatment	215:250	high temperature alkali pretreatment with different alkaline loading and temperatures	215:299	Experiments have been performed for pretreatment of sorghum, wheat straw and bamboo through high temperature alkali pretreatment with different alkaline loading and temperatures, and the data on extent of delignification in terms of the final compositions of cellulose, hemicellulose and lignin have been generated.
27686723	3	28	theme	parameters	600:609	arg1	effect					570:575	The effect	566:575	The effect of different operating parameters on the extent of delignification and cellulose conversion	566:667	The effect of different operating parameters on the extent of delignification and cellulose conversion are evaluated.
27686723	1	29	theme	temperature	220:230	arg1	pretreatment					239:250	high temperature alkali pretreatment	215:250	high temperature alkali pretreatment with different alkaline loading and temperatures	215:299	Experiments have been performed for pretreatment of sorghum, wheat straw and bamboo through high temperature alkali pretreatment with different alkaline loading and temperatures, and the data on extent of delignification in terms of the final compositions of cellulose, hemicellulose and lignin have been generated.
27686723	0	30	theme	saccharification	30:45	arg1	modeling					85:92	kinetic modeling	77:92	kinetic modeling of sorghum saccharification	77:120	Modelling of pretreatment and saccharification with different feedstocks and kinetic modeling of sorghum saccharification.
27686723	0	30	theme	saccharification	30:45	arg1	Modelling					0:8	Modelling	0:8	Modelling of pretreatment and saccharification with different feedstocks	0:71	Modelling of pretreatment and saccharification with different feedstocks and kinetic modeling of sorghum saccharification.
27686723	2	31	theme	conversion	535:544	arg1	extent					525:530	the extent	521:530	the extent of conversion possible after 72h	521:563	Further, enzymatic saccharification has been carried out in all the cases to find the extent of conversion possible after 72h.
27686723	1	32	theme	lignin	411:416	arg1	compositions					366:377	the final compositions	356:377	the final compositions of cellulose, hemicellulose and lignin	356:416	Experiments have been performed for pretreatment of sorghum, wheat straw and bamboo through high temperature alkali pretreatment with different alkaline loading and temperatures, and the data on extent of delignification in terms of the final compositions of cellulose, hemicellulose and lignin have been generated.
27686723	1	33	theme	alkali	232:237	arg1	pretreatment					239:250	high temperature alkali pretreatment	215:250	high temperature alkali pretreatment with different alkaline loading and temperatures	215:299	Experiments have been performed for pretreatment of sorghum, wheat straw and bamboo through high temperature alkali pretreatment with different alkaline loading and temperatures, and the data on extent of delignification in terms of the final compositions of cellulose, hemicellulose and lignin have been generated.
27686723	1	34	from	terms	347:351	arg1	extent					318:323	extent	318:323	extent of delignification in terms of the final compositions of cellulose, hemicellulose and lignin	318:416	Experiments have been performed for pretreatment of sorghum, wheat straw and bamboo through high temperature alkali pretreatment with different alkaline loading and temperatures, and the data on extent of delignification in terms of the final compositions of cellulose, hemicellulose and lignin have been generated.
27686723	3	35	theme	cellulose	648:656	arg1	extent					618:623	the extent	614:623	the extent of delignification and cellulose conversion	614:667	The effect of different operating parameters on the extent of delignification and cellulose conversion are evaluated.
27686723	3	36	theme	different	580:588	arg1	parameters					600:609	different operating parameters	580:609	different operating parameters	580:609	The effect of different operating parameters on the extent of delignification and cellulose conversion are evaluated.
27686723	0	37	with	pretreatment	13:24	arg1	feedstocks					62:71	different feedstocks	52:71	different feedstocks	52:71	Modelling of pretreatment and saccharification with different feedstocks and kinetic modeling of sorghum saccharification.
27686723	4	38	theme	generalized	719:729	arg1	models					778:783	a generalized multi-feedstock and individual feedstock based models	717:783	a generalized multi-feedstock and individual feedstock based models which can be used to determine the extent of delignification and cellulose conversion for any and specific biomass respectively with alkaline pretreatment and similar enzyme conditions as considered in the present study	717:1003	This data is employed to develop a generalized multi-feedstock and individual feedstock based models which can be used to determine the extent of delignification and cellulose conversion for any and specific biomass respectively with alkaline pretreatment and similar enzyme conditions as considered in the present study.
27686723	0	39	theme	different	52:60	arg1	feedstocks					62:71	different feedstocks	52:71	different feedstocks	52:71	Modelling of pretreatment and saccharification with different feedstocks and kinetic modeling of sorghum saccharification.
27686723	1	40	theme	delignification	328:342	arg1	extent					318:323	extent	318:323	extent of delignification in terms of the final compositions of cellulose, hemicellulose and lignin	318:416	Experiments have been performed for pretreatment of sorghum, wheat straw and bamboo through high temperature alkali pretreatment with different alkaline loading and temperatures, and the data on extent of delignification in terms of the final compositions of cellulose, hemicellulose and lignin have been generated.
27686723	4	41	theme	alkaline	918:925	arg1	pretreatment					927:938	alkaline pretreatment	918:938	alkaline pretreatment	918:938	This data is employed to develop a generalized multi-feedstock and individual feedstock based models which can be used to determine the extent of delignification and cellulose conversion for any and specific biomass respectively with alkaline pretreatment and similar enzyme conditions as considered in the present study.
27686723	1	42	dep	straw	190:194	arg1	wheat					184:188	sorghum, wheat straw	175:194	wheat	184:188	Experiments have been performed for pretreatment of sorghum, wheat straw and bamboo through high temperature alkali pretreatment with different alkaline loading and temperatures, and the data on extent of delignification in terms of the final compositions of cellulose, hemicellulose and lignin have been generated.
27686723	4	43	theme	similar	944:950	arg1	enzyme					952:957	similar enzyme	944:957	similar enzyme	944:957	This data is employed to develop a generalized multi-feedstock and individual feedstock based models which can be used to determine the extent of delignification and cellulose conversion for any and specific biomass respectively with alkaline pretreatment and similar enzyme conditions as considered in the present study.
27686723	5	44	theme	cellulosic	1071:1080	arg1	conversion					1082:1091	cellulosic conversion	1071:1091	cellulosic conversion	1071:1091	Also, a kinetic model is developed and validated for sorghum for cellulosic conversion.
27686723	5	45	theme	kinetic	1014:1020	arg1	model					1022:1026	a kinetic model	1012:1026	a kinetic model	1012:1026	Also, a kinetic model is developed and validated for sorghum for cellulosic conversion.
27686723	4	46	used	used	798:801	arg2	models					778:783	a generalized multi-feedstock and individual feedstock based models	717:783	a generalized multi-feedstock and individual feedstock based models which can be used to determine the extent of delignification and cellulose conversion for any and specific biomass respectively with alkaline pretreatment and similar enzyme conditions as considered in the present study	717:1003	This data is employed to develop a generalized multi-feedstock and individual feedstock based models which can be used to determine the extent of delignification and cellulose conversion for any and specific biomass respectively with alkaline pretreatment and similar enzyme conditions as considered in the present study.
27686723	3	47	theme	delignification	628:642	arg1	extent					618:623	the extent	614:623	the extent of delignification and cellulose conversion	614:667	The effect of different operating parameters on the extent of delignification and cellulose conversion are evaluated.
27686723	4	48	theme	multi-feedstock	731:745	arg1	models					778:783	a generalized multi-feedstock and individual feedstock based models	717:783	a generalized multi-feedstock and individual feedstock based models which can be used to determine the extent of delignification and cellulose conversion for any and specific biomass respectively with alkaline pretreatment and similar enzyme conditions as considered in the present study	717:1003	This data is employed to develop a generalized multi-feedstock and individual feedstock based models which can be used to determine the extent of delignification and cellulose conversion for any and specific biomass respectively with alkaline pretreatment and similar enzyme conditions as considered in the present study.
27686723	3	49	from	effect	570:575	arg1	extent					618:623	the extent	614:623	the extent of delignification and cellulose conversion	614:667	The effect of different operating parameters on the extent of delignification and cellulose conversion are evaluated.
27686723	0	50	theme	kinetic	77:83	arg1	modeling					85:92	kinetic modeling	77:92	kinetic modeling of sorghum saccharification	77:120	Modelling of pretreatment and saccharification with different feedstocks and kinetic modeling of sorghum saccharification.
27686723	3	51	dep	delignification	628:642	arg1	conversion					658:667	conversion	658:667	conversion	658:667	The effect of different operating parameters on the extent of delignification and cellulose conversion are evaluated.
27686723	2	52	dep	conversion	535:544	arg1	possible					546:553	possible	546:553	possible	546:553	Further, enzymatic saccharification has been carried out in all the cases to find the extent of conversion possible after 72h.
27686723	1	53	theme	different	257:265	arg1	loading					276:282	different alkaline loading	257:282	different alkaline loading	257:282	Experiments have been performed for pretreatment of sorghum, wheat straw and bamboo through high temperature alkali pretreatment with different alkaline loading and temperatures, and the data on extent of delignification in terms of the final compositions of cellulose, hemicellulose and lignin have been generated.
27686723	1	54	with	pretreatment	239:250	arg1	temperatures					288:299	temperatures	288:299	temperatures	288:299	Experiments have been performed for pretreatment of sorghum, wheat straw and bamboo through high temperature alkali pretreatment with different alkaline loading and temperatures, and the data on extent of delignification in terms of the final compositions of cellulose, hemicellulose and lignin have been generated.
27686723	1	54	with	pretreatment	239:250	arg1	loading					276:282	different alkaline loading	257:282	different alkaline loading	257:282	Experiments have been performed for pretreatment of sorghum, wheat straw and bamboo through high temperature alkali pretreatment with different alkaline loading and temperatures, and the data on extent of delignification in terms of the final compositions of cellulose, hemicellulose and lignin have been generated.
27686723	1	55	theme	alkaline	267:274	arg1	loading					276:282	different alkaline loading	257:282	different alkaline loading	257:282	Experiments have been performed for pretreatment of sorghum, wheat straw and bamboo through high temperature alkali pretreatment with different alkaline loading and temperatures, and the data on extent of delignification in terms of the final compositions of cellulose, hemicellulose and lignin have been generated.
27357535	7	0	theme	vessel	1207:1212	arg1	formation					1214:1222	new blood vessel formation	1197:1222	new blood vessel formation	1197:1222	After surgical implantation into rabbit calvarial bone defects, new blood vessel formation was significantly higher with MK1-EPS composite bone granules than with granules alone, and new bone formation increased significantly.
27357535	3	1	theme	tube	480:483	arg1	formation					485:493	vascular tube formation	471:493	vascular tube formation	471:493	MK1-EPS significantly increased human umbilical vein endothelial cell (HUVEC) proliferation, migration, and vascular tube formation.
27357535	3	1	theme	tube	480:483	arg1	HUVEC					434:438	human umbilical vein endothelial cell (HUVEC)	395:439	human umbilical vein endothelial cell (HUVEC) proliferation, migration, and vascular tube formation	395:493	MK1-EPS significantly increased human umbilical vein endothelial cell (HUVEC) proliferation, migration, and vascular tube formation.
27357535	5	2	theme	kinase	848:853	arg1	AKT					858:860	AKT	858:860	AKT	858:860	In addition, the expression of p21 and intercellular adhesion molecule 1 (ICAM1), and phosphorylation of signal transducer and activator of transcription 3 (STAT3), but not of protein kinase B (AKT), were increased.
27357535	5	2	theme	kinase	848:853	arg1	B					855:855	protein kinase B	840:855	protein kinase B (AKT)	840:861	In addition, the expression of p21 and intercellular adhesion molecule 1 (ICAM1), and phosphorylation of signal transducer and activator of transcription 3 (STAT3), but not of protein kinase B (AKT), were increased.
27357535	8	3	theme	bone	1463:1466	arg1	engineering					1475:1485	bone tissue engineering	1463:1485	bone tissue engineering	1463:1485	Therefore, MK1-EPS induces angiogenesis and may have potential for use as a bone regeneration agent in bone tissue engineering applications.
27357535	6	4	theme	p38	903:905	arg1	inhibitors					889:898	Specific inhibitors	880:898	Specific inhibitors of p38 (SB203580), ERK (PD98059), and JNK (SP600125)	880:951	Specific inhibitors of p38 (SB203580), ERK (PD98059), and JNK (SP600125) inhibited MK1-EPS-induced HUVEC proliferation, tube formation, and cell migration, and partially attenuated MKI-EPS-induced expression of p21 and ICAM1, and STAT3 phosphorylation.
27357535	2	5	theme	bacterial	313:321	arg1	strain					323:328	a novel bacterial strain	305:328	a novel bacterial strain isolated from Neungee mushrooms	305:360	Here, we evaluated the angiogenic properties of an exopolysaccharide (EPS) secreted by MK1 (MK1-EPS), a novel bacterial strain isolated from Neungee mushrooms.
27357535	2	5	theme	bacterial	313:321	arg1	MK1					290:292	MK1	290:292	MK1 (MK1-EPS)	290:302	Here, we evaluated the angiogenic properties of an exopolysaccharide (EPS) secreted by MK1 (MK1-EPS), a novel bacterial strain isolated from Neungee mushrooms.
27357535	0	6	theme	bacterial	86:94	arg1	strain					96:101	the MK1 bacterial strain	78:101	the MK1 bacterial strain	78:101	Evaluation of the angiogenic potency of a novel exopolysaccharide produced by the MK1 bacterial strain.
27357535	7	7	theme	MK1-EPS	1254:1260	arg1	granules					1277:1284	MK1-EPS composite bone granules	1254:1284	MK1-EPS composite bone granules	1254:1284	After surgical implantation into rabbit calvarial bone defects, new blood vessel formation was significantly higher with MK1-EPS composite bone granules than with granules alone, and new bone formation increased significantly.
27357535	6	8	theme	MKI-EPS-induced	1061:1075	arg1	expression					1077:1086	MKI-EPS-induced expression	1061:1086	MKI-EPS-induced expression of p21 and ICAM1	1061:1103	Specific inhibitors of p38 (SB203580), ERK (PD98059), and JNK (SP600125) inhibited MK1-EPS-induced HUVEC proliferation, tube formation, and cell migration, and partially attenuated MKI-EPS-induced expression of p21 and ICAM1, and STAT3 phosphorylation.
27357535	4	9	theme	c-Jun	579:583	arg1	kinase					596:601	c-Jun N-terminal kinase	579:601	c-Jun N-terminal kinase (JNK)	579:607	MK1-EPS enhanced the phosphorylation of extracellular signal-related kinase (ERK), c-Jun N-terminal kinase (JNK), and p38, which are mitogen-activated protein kinases.
27357535	4	9	theme	c-Jun	579:583	arg1	JNK					604:606	JNK	604:606	JNK	604:606	MK1-EPS enhanced the phosphorylation of extracellular signal-related kinase (ERK), c-Jun N-terminal kinase (JNK), and p38, which are mitogen-activated protein kinases.
27357535	0	10	theme	MK1	82:84	arg1	strain					96:101	the MK1 bacterial strain	78:101	the MK1 bacterial strain	78:101	Evaluation of the angiogenic potency of a novel exopolysaccharide produced by the MK1 bacterial strain.
27357535	6	11	theme	MK1-EPS-induced	963:977	arg1	proliferation					985:997	MK1-EPS-induced HUVEC proliferation	963:997	MK1-EPS-induced HUVEC proliferation	963:997	Specific inhibitors of p38 (SB203580), ERK (PD98059), and JNK (SP600125) inhibited MK1-EPS-induced HUVEC proliferation, tube formation, and cell migration, and partially attenuated MKI-EPS-induced expression of p21 and ICAM1, and STAT3 phosphorylation.
27357535	5	12	theme	protein	840:846	arg1	AKT					858:860	AKT	858:860	AKT	858:860	In addition, the expression of p21 and intercellular adhesion molecule 1 (ICAM1), and phosphorylation of signal transducer and activator of transcription 3 (STAT3), but not of protein kinase B (AKT), were increased.
27357535	5	12	theme	protein	840:846	arg1	B					855:855	protein kinase B	840:855	protein kinase B (AKT)	840:861	In addition, the expression of p21 and intercellular adhesion molecule 1 (ICAM1), and phosphorylation of signal transducer and activator of transcription 3 (STAT3), but not of protein kinase B (AKT), were increased.
27357535	1	13	from	step	147:150	arg1	healing					161:167	wound healing	155:167	wound healing	155:167	Angiogenesis is an essential physiological step in wound healing and other regenerative processes.
27357535	1	13	from	step	147:150	arg1	processes					192:200	other regenerative processes	173:200	other regenerative processes	173:200	Angiogenesis is an essential physiological step in wound healing and other regenerative processes.
27357535	1	14	theme	other	173:177	arg1	processes					192:200	other regenerative processes	173:200	other regenerative processes	173:200	Angiogenesis is an essential physiological step in wound healing and other regenerative processes.
27357535	3	15	theme	cell	428:431	arg1	formation					485:493	vascular tube formation	471:493	vascular tube formation	471:493	MK1-EPS significantly increased human umbilical vein endothelial cell (HUVEC) proliferation, migration, and vascular tube formation.
27357535	3	15	theme	cell	428:431	arg1	migration					456:464	migration	456:464	migration	456:464	MK1-EPS significantly increased human umbilical vein endothelial cell (HUVEC) proliferation, migration, and vascular tube formation.
27357535	3	15	theme	cell	428:431	arg1	proliferation					441:453	proliferation	441:453	proliferation	441:453	MK1-EPS significantly increased human umbilical vein endothelial cell (HUVEC) proliferation, migration, and vascular tube formation.
27357535	3	15	theme	cell	428:431	arg1	HUVEC					434:438	human umbilical vein endothelial cell (HUVEC)	395:439	human umbilical vein endothelial cell (HUVEC) proliferation, migration, and vascular tube formation	395:493	MK1-EPS significantly increased human umbilical vein endothelial cell (HUVEC) proliferation, migration, and vascular tube formation.
27357535	5	16	theme	intercellular	703:715	arg1	molecule					726:733	intercellular adhesion molecule 1	703:735	intercellular adhesion molecule 1 (ICAM1)	703:743	In addition, the expression of p21 and intercellular adhesion molecule 1 (ICAM1), and phosphorylation of signal transducer and activator of transcription 3 (STAT3), but not of protein kinase B (AKT), were increased.
27357535	5	16	theme	intercellular	703:715	arg1	ICAM1					738:742	ICAM1	738:742	ICAM1	738:742	In addition, the expression of p21 and intercellular adhesion molecule 1 (ICAM1), and phosphorylation of signal transducer and activator of transcription 3 (STAT3), but not of protein kinase B (AKT), were increased.
27357535	3	17	theme	endothelial	416:426	arg1	formation					485:493	vascular tube formation	471:493	vascular tube formation	471:493	MK1-EPS significantly increased human umbilical vein endothelial cell (HUVEC) proliferation, migration, and vascular tube formation.
27357535	3	17	theme	endothelial	416:426	arg1	migration					456:464	migration	456:464	migration	456:464	MK1-EPS significantly increased human umbilical vein endothelial cell (HUVEC) proliferation, migration, and vascular tube formation.
27357535	3	17	theme	endothelial	416:426	arg1	proliferation					441:453	proliferation	441:453	proliferation	441:453	MK1-EPS significantly increased human umbilical vein endothelial cell (HUVEC) proliferation, migration, and vascular tube formation.
27357535	3	17	theme	endothelial	416:426	arg1	HUVEC					434:438	human umbilical vein endothelial cell (HUVEC)	395:439	human umbilical vein endothelial cell (HUVEC) proliferation, migration, and vascular tube formation	395:493	MK1-EPS significantly increased human umbilical vein endothelial cell (HUVEC) proliferation, migration, and vascular tube formation.
27357535	6	18	theme	ERK	919:921	arg1	inhibitors					889:898	Specific inhibitors	880:898	Specific inhibitors of p38 (SB203580), ERK (PD98059), and JNK (SP600125)	880:951	Specific inhibitors of p38 (SB203580), ERK (PD98059), and JNK (SP600125) inhibited MK1-EPS-induced HUVEC proliferation, tube formation, and cell migration, and partially attenuated MKI-EPS-induced expression of p21 and ICAM1, and STAT3 phosphorylation.
27357535	8	19	theme	bone	1436:1439	arg1	agent					1454:1458	a bone regeneration agent	1434:1458	a bone regeneration agent	1434:1458	Therefore, MK1-EPS induces angiogenesis and may have potential for use as a bone regeneration agent in bone tissue engineering applications.
27357535	7	20	theme	surgical	1139:1146	arg1	implantation					1148:1159	surgical implantation	1139:1159	surgical implantation into rabbit calvarial bone defects	1139:1194	After surgical implantation into rabbit calvarial bone defects, new blood vessel formation was significantly higher with MK1-EPS composite bone granules than with granules alone, and new bone formation increased significantly.
27357535	3	21	theme	vein	411:414	arg1	formation					485:493	vascular tube formation	471:493	vascular tube formation	471:493	MK1-EPS significantly increased human umbilical vein endothelial cell (HUVEC) proliferation, migration, and vascular tube formation.
27357535	3	21	theme	vein	411:414	arg1	migration					456:464	migration	456:464	migration	456:464	MK1-EPS significantly increased human umbilical vein endothelial cell (HUVEC) proliferation, migration, and vascular tube formation.
27357535	3	21	theme	vein	411:414	arg1	proliferation					441:453	proliferation	441:453	proliferation	441:453	MK1-EPS significantly increased human umbilical vein endothelial cell (HUVEC) proliferation, migration, and vascular tube formation.
27357535	3	21	theme	vein	411:414	arg1	HUVEC					434:438	human umbilical vein endothelial cell (HUVEC)	395:439	human umbilical vein endothelial cell (HUVEC) proliferation, migration, and vascular tube formation	395:493	MK1-EPS significantly increased human umbilical vein endothelial cell (HUVEC) proliferation, migration, and vascular tube formation.
27357535	8	22	theme	potential	1413:1421	arg1	applications					1487:1498	potential for use as a bone regeneration agent in bone tissue engineering applications	1413:1498	potential for use as a bone regeneration agent in bone tissue engineering applications	1413:1498	Therefore, MK1-EPS induces angiogenesis and may have potential for use as a bone regeneration agent in bone tissue engineering applications.
27357535	4	23	theme	signal-related	550:563	arg1	ERK					573:575	ERK	573:575	ERK	573:575	MK1-EPS enhanced the phosphorylation of extracellular signal-related kinase (ERK), c-Jun N-terminal kinase (JNK), and p38, which are mitogen-activated protein kinases.
27357535	4	23	theme	signal-related	550:563	arg1	kinase					565:570	extracellular signal-related kinase	536:570	extracellular signal-related kinase (ERK)	536:576	MK1-EPS enhanced the phosphorylation of extracellular signal-related kinase (ERK), c-Jun N-terminal kinase (JNK), and p38, which are mitogen-activated protein kinases.
27357535	8	24	contain	have	1408:1411	arg2	applications					1487:1498	potential for use as a bone regeneration agent in bone tissue engineering applications	1413:1498	potential for use as a bone regeneration agent in bone tissue engineering applications	1413:1498	Therefore, MK1-EPS induces angiogenesis and may have potential for use as a bone regeneration agent in bone tissue engineering applications.
27357535	8	24	contain	have	1408:1411	arg1	MK1-EPS					1371:1377	MK1-EPS	1371:1377	MK1-EPS	1371:1377	Therefore, MK1-EPS induces angiogenesis and may have potential for use as a bone regeneration agent in bone tissue engineering applications.
27357535	2	25	theme	Neungee	344:350	arg1	mushrooms					352:360	Neungee mushrooms	344:360	Neungee mushrooms	344:360	Here, we evaluated the angiogenic properties of an exopolysaccharide (EPS) secreted by MK1 (MK1-EPS), a novel bacterial strain isolated from Neungee mushrooms.
27357535	0	26	theme	angiogenic	18:27	arg1	potency					29:35	the angiogenic potency	14:35	the angiogenic potency of a novel exopolysaccharide	14:64	Evaluation of the angiogenic potency of a novel exopolysaccharide produced by the MK1 bacterial strain.
27357535	4	27	theme	extracellular	536:548	arg1	ERK					573:575	ERK	573:575	ERK	573:575	MK1-EPS enhanced the phosphorylation of extracellular signal-related kinase (ERK), c-Jun N-terminal kinase (JNK), and p38, which are mitogen-activated protein kinases.
27357535	4	27	theme	extracellular	536:548	arg1	kinase					565:570	extracellular signal-related kinase	536:570	extracellular signal-related kinase (ERK)	536:576	MK1-EPS enhanced the phosphorylation of extracellular signal-related kinase (ERK), c-Jun N-terminal kinase (JNK), and p38, which are mitogen-activated protein kinases.
27357535	7	28	theme	bone	1272:1275	arg1	granules					1277:1284	MK1-EPS composite bone granules	1254:1284	MK1-EPS composite bone granules	1254:1284	After surgical implantation into rabbit calvarial bone defects, new blood vessel formation was significantly higher with MK1-EPS composite bone granules than with granules alone, and new bone formation increased significantly.
27357535	6	29	theme	cell	1020:1023	arg1	migration					1025:1033	cell migration	1020:1033	cell migration	1020:1033	Specific inhibitors of p38 (SB203580), ERK (PD98059), and JNK (SP600125) inhibited MK1-EPS-induced HUVEC proliferation, tube formation, and cell migration, and partially attenuated MKI-EPS-induced expression of p21 and ICAM1, and STAT3 phosphorylation.
27357535	6	30	theme	STAT3	1110:1114	arg1	phosphorylation					1116:1130	STAT3 phosphorylation	1110:1130	STAT3 phosphorylation	1110:1130	Specific inhibitors of p38 (SB203580), ERK (PD98059), and JNK (SP600125) inhibited MK1-EPS-induced HUVEC proliferation, tube formation, and cell migration, and partially attenuated MKI-EPS-induced expression of p21 and ICAM1, and STAT3 phosphorylation.
27357535	7	31	theme	blood	1201:1205	arg1	formation					1214:1222	new blood vessel formation	1197:1222	new blood vessel formation	1197:1222	After surgical implantation into rabbit calvarial bone defects, new blood vessel formation was significantly higher with MK1-EPS composite bone granules than with granules alone, and new bone formation increased significantly.
27357535	5	32	theme	transducer	776:785	arg1	phosphorylation					750:764	phosphorylation	750:764	phosphorylation of signal transducer and activator of transcription 3 (STAT3), but not of protein kinase B (AKT),	750:862	In addition, the expression of p21 and intercellular adhesion molecule 1 (ICAM1), and phosphorylation of signal transducer and activator of transcription 3 (STAT3), but not of protein kinase B (AKT), were increased.
27357535	5	32	theme	transducer	776:785	arg1	expression					681:690	the expression	677:690	the expression of p21 and intercellular adhesion molecule 1 (ICAM1)	677:743	In addition, the expression of p21 and intercellular adhesion molecule 1 (ICAM1), and phosphorylation of signal transducer and activator of transcription 3 (STAT3), but not of protein kinase B (AKT), were increased.
27357535	1	33	theme	wound	155:159	arg1	healing					161:167	wound healing	155:167	wound healing	155:167	Angiogenesis is an essential physiological step in wound healing and other regenerative processes.
27357535	7	34	theme	new	1197:1199	arg1	formation					1214:1222	new blood vessel formation	1197:1222	new blood vessel formation	1197:1222	After surgical implantation into rabbit calvarial bone defects, new blood vessel formation was significantly higher with MK1-EPS composite bone granules than with granules alone, and new bone formation increased significantly.
27357535	3	35	theme	human	395:399	arg1	formation					485:493	vascular tube formation	471:493	vascular tube formation	471:493	MK1-EPS significantly increased human umbilical vein endothelial cell (HUVEC) proliferation, migration, and vascular tube formation.
27357535	3	35	theme	human	395:399	arg1	migration					456:464	migration	456:464	migration	456:464	MK1-EPS significantly increased human umbilical vein endothelial cell (HUVEC) proliferation, migration, and vascular tube formation.
27357535	3	35	theme	human	395:399	arg1	proliferation					441:453	proliferation	441:453	proliferation	441:453	MK1-EPS significantly increased human umbilical vein endothelial cell (HUVEC) proliferation, migration, and vascular tube formation.
27357535	3	35	theme	human	395:399	arg1	HUVEC					434:438	human umbilical vein endothelial cell (HUVEC)	395:439	human umbilical vein endothelial cell (HUVEC) proliferation, migration, and vascular tube formation	395:493	MK1-EPS significantly increased human umbilical vein endothelial cell (HUVEC) proliferation, migration, and vascular tube formation.
27357535	4	36	theme	kinase	565:570	arg1	phosphorylation					517:531	the phosphorylation	513:531	the phosphorylation	513:531	MK1-EPS enhanced the phosphorylation of extracellular signal-related kinase (ERK), c-Jun N-terminal kinase (JNK), and p38, which are mitogen-activated protein kinases.
27357535	4	36	theme	kinase	565:570	arg1	kinases					655:661	mitogen-activated protein kinases	629:661	mitogen-activated protein kinases	629:661	MK1-EPS enhanced the phosphorylation of extracellular signal-related kinase (ERK), c-Jun N-terminal kinase (JNK), and p38, which are mitogen-activated protein kinases.
27357535	7	37	theme	bone	1183:1186	arg1	defects					1188:1194	rabbit calvarial bone defects	1166:1194	rabbit calvarial bone defects	1166:1194	After surgical implantation into rabbit calvarial bone defects, new blood vessel formation was significantly higher with MK1-EPS composite bone granules than with granules alone, and new bone formation increased significantly.
27357535	2	38	theme	angiogenic	226:235	arg1	properties					237:246	the angiogenic properties	222:246	the angiogenic properties of an exopolysaccharide (EPS) secreted by MK1 (MK1-EPS), a novel bacterial strain isolated from Neungee mushrooms	222:360	Here, we evaluated the angiogenic properties of an exopolysaccharide (EPS) secreted by MK1 (MK1-EPS), a novel bacterial strain isolated from Neungee mushrooms.
27357535	1	39	theme	regenerative	179:190	arg1	processes					192:200	other regenerative processes	173:200	other regenerative processes	173:200	Angiogenesis is an essential physiological step in wound healing and other regenerative processes.
27357535	4	40	theme	p38	614:616	arg1	phosphorylation					517:531	the phosphorylation	513:531	the phosphorylation	513:531	MK1-EPS enhanced the phosphorylation of extracellular signal-related kinase (ERK), c-Jun N-terminal kinase (JNK), and p38, which are mitogen-activated protein kinases.
27357535	4	40	theme	p38	614:616	arg1	kinases					655:661	mitogen-activated protein kinases	629:661	mitogen-activated protein kinases	629:661	MK1-EPS enhanced the phosphorylation of extracellular signal-related kinase (ERK), c-Jun N-terminal kinase (JNK), and p38, which are mitogen-activated protein kinases.
27357535	0	41	theme	potency	29:35	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of the angiogenic potency of a novel exopolysaccharide	0:64	Evaluation of the angiogenic potency of a novel exopolysaccharide produced by the MK1 bacterial strain.
27357535	8	42	theme	tissue	1468:1473	arg1	engineering					1475:1485	bone tissue engineering	1463:1485	bone tissue engineering	1463:1485	Therefore, MK1-EPS induces angiogenesis and may have potential for use as a bone regeneration agent in bone tissue engineering applications.
27357535	3	43	theme	umbilical	401:409	arg1	formation					485:493	vascular tube formation	471:493	vascular tube formation	471:493	MK1-EPS significantly increased human umbilical vein endothelial cell (HUVEC) proliferation, migration, and vascular tube formation.
27357535	3	43	theme	umbilical	401:409	arg1	migration					456:464	migration	456:464	migration	456:464	MK1-EPS significantly increased human umbilical vein endothelial cell (HUVEC) proliferation, migration, and vascular tube formation.
27357535	3	43	theme	umbilical	401:409	arg1	proliferation					441:453	proliferation	441:453	proliferation	441:453	MK1-EPS significantly increased human umbilical vein endothelial cell (HUVEC) proliferation, migration, and vascular tube formation.
27357535	3	43	theme	umbilical	401:409	arg1	HUVEC					434:438	human umbilical vein endothelial cell (HUVEC)	395:439	human umbilical vein endothelial cell (HUVEC) proliferation, migration, and vascular tube formation	395:493	MK1-EPS significantly increased human umbilical vein endothelial cell (HUVEC) proliferation, migration, and vascular tube formation.
27357535	5	44	theme	signal	769:774	arg1	STAT3					821:825	STAT3	821:825	STAT3	821:825	In addition, the expression of p21 and intercellular adhesion molecule 1 (ICAM1), and phosphorylation of signal transducer and activator of transcription 3 (STAT3), but not of protein kinase B (AKT), were increased.
27357535	5	44	theme	signal	769:774	arg1	transducer					776:785	signal transducer	769:785	signal transducer	769:785	In addition, the expression of p21 and intercellular adhesion molecule 1 (ICAM1), and phosphorylation of signal transducer and activator of transcription 3 (STAT3), but not of protein kinase B (AKT), were increased.
27357535	5	45	theme	transcription	804:816	arg1	STAT3					821:825	STAT3	821:825	STAT3	821:825	In addition, the expression of p21 and intercellular adhesion molecule 1 (ICAM1), and phosphorylation of signal transducer and activator of transcription 3 (STAT3), but not of protein kinase B (AKT), were increased.
27357535	5	45	theme	transcription	804:816	arg1	transducer					776:785	signal transducer	769:785	signal transducer	769:785	In addition, the expression of p21 and intercellular adhesion molecule 1 (ICAM1), and phosphorylation of signal transducer and activator of transcription 3 (STAT3), but not of protein kinase B (AKT), were increased.
27357535	0	46	theme	novel	42:46	arg1	exopolysaccharide					48:64	a novel exopolysaccharide	40:64	a novel exopolysaccharide	40:64	Evaluation of the angiogenic potency of a novel exopolysaccharide produced by the MK1 bacterial strain.
27357535	4	47	theme	mitogen-activated	629:645	arg1	phosphorylation					517:531	the phosphorylation	513:531	the phosphorylation	513:531	MK1-EPS enhanced the phosphorylation of extracellular signal-related kinase (ERK), c-Jun N-terminal kinase (JNK), and p38, which are mitogen-activated protein kinases.
27357535	4	47	theme	mitogen-activated	629:645	arg1	kinases					655:661	mitogen-activated protein kinases	629:661	mitogen-activated protein kinases	629:661	MK1-EPS enhanced the phosphorylation of extracellular signal-related kinase (ERK), c-Jun N-terminal kinase (JNK), and p38, which are mitogen-activated protein kinases.
27357535	5	48	theme	molecule	726:733	arg1	phosphorylation					750:764	phosphorylation	750:764	phosphorylation of signal transducer and activator of transcription 3 (STAT3), but not of protein kinase B (AKT),	750:862	In addition, the expression of p21 and intercellular adhesion molecule 1 (ICAM1), and phosphorylation of signal transducer and activator of transcription 3 (STAT3), but not of protein kinase B (AKT), were increased.
27357535	5	48	theme	molecule	726:733	arg1	expression					681:690	the expression	677:690	the expression of p21 and intercellular adhesion molecule 1 (ICAM1)	677:743	In addition, the expression of p21 and intercellular adhesion molecule 1 (ICAM1), and phosphorylation of signal transducer and activator of transcription 3 (STAT3), but not of protein kinase B (AKT), were increased.
27357535	7	49	theme	composite	1262:1270	arg1	granules					1277:1284	MK1-EPS composite bone granules	1254:1284	MK1-EPS composite bone granules	1254:1284	After surgical implantation into rabbit calvarial bone defects, new blood vessel formation was significantly higher with MK1-EPS composite bone granules than with granules alone, and new bone formation increased significantly.
27357535	6	50	theme	tube	1000:1003	arg1	formation					1005:1013	tube formation	1000:1013	tube formation	1000:1013	Specific inhibitors of p38 (SB203580), ERK (PD98059), and JNK (SP600125) inhibited MK1-EPS-induced HUVEC proliferation, tube formation, and cell migration, and partially attenuated MKI-EPS-induced expression of p21 and ICAM1, and STAT3 phosphorylation.
27357535	2	51	theme	novel	307:311	arg1	strain					323:328	a novel bacterial strain	305:328	a novel bacterial strain isolated from Neungee mushrooms	305:360	Here, we evaluated the angiogenic properties of an exopolysaccharide (EPS) secreted by MK1 (MK1-EPS), a novel bacterial strain isolated from Neungee mushrooms.
27357535	2	51	theme	novel	307:311	arg1	MK1					290:292	MK1	290:292	MK1 (MK1-EPS)	290:302	Here, we evaluated the angiogenic properties of an exopolysaccharide (EPS) secreted by MK1 (MK1-EPS), a novel bacterial strain isolated from Neungee mushrooms.
27357535	8	52	from	use	1427:1429	arg1	engineering					1475:1485	bone tissue engineering	1463:1485	bone tissue engineering	1463:1485	Therefore, MK1-EPS induces angiogenesis and may have potential for use as a bone regeneration agent in bone tissue engineering applications.
27357535	6	53	theme	JNK	938:940	arg1	inhibitors					889:898	Specific inhibitors	880:898	Specific inhibitors of p38 (SB203580), ERK (PD98059), and JNK (SP600125)	880:951	Specific inhibitors of p38 (SB203580), ERK (PD98059), and JNK (SP600125) inhibited MK1-EPS-induced HUVEC proliferation, tube formation, and cell migration, and partially attenuated MKI-EPS-induced expression of p21 and ICAM1, and STAT3 phosphorylation.
27357535	6	54	theme	ICAM1	1099:1103	arg1	expression					1077:1086	MKI-EPS-induced expression	1061:1086	MKI-EPS-induced expression of p21 and ICAM1	1061:1103	Specific inhibitors of p38 (SB203580), ERK (PD98059), and JNK (SP600125) inhibited MK1-EPS-induced HUVEC proliferation, tube formation, and cell migration, and partially attenuated MKI-EPS-induced expression of p21 and ICAM1, and STAT3 phosphorylation.
27357535	6	54	theme	ICAM1	1099:1103	arg1	phosphorylation					1116:1130	STAT3 phosphorylation	1110:1130	STAT3 phosphorylation	1110:1130	Specific inhibitors of p38 (SB203580), ERK (PD98059), and JNK (SP600125) inhibited MK1-EPS-induced HUVEC proliferation, tube formation, and cell migration, and partially attenuated MKI-EPS-induced expression of p21 and ICAM1, and STAT3 phosphorylation.
27357535	7	55	theme	rabbit	1166:1171	arg1	defects					1188:1194	rabbit calvarial bone defects	1166:1194	rabbit calvarial bone defects	1166:1194	After surgical implantation into rabbit calvarial bone defects, new blood vessel formation was significantly higher with MK1-EPS composite bone granules than with granules alone, and new bone formation increased significantly.
27357535	3	56	theme	vascular	471:478	arg1	formation					485:493	vascular tube formation	471:493	vascular tube formation	471:493	MK1-EPS significantly increased human umbilical vein endothelial cell (HUVEC) proliferation, migration, and vascular tube formation.
27357535	3	56	theme	vascular	471:478	arg1	HUVEC					434:438	human umbilical vein endothelial cell (HUVEC)	395:439	human umbilical vein endothelial cell (HUVEC) proliferation, migration, and vascular tube formation	395:493	MK1-EPS significantly increased human umbilical vein endothelial cell (HUVEC) proliferation, migration, and vascular tube formation.
27357535	7	57	theme	bone	1320:1323	arg1	formation					1325:1333	new bone formation	1316:1333	new bone formation	1316:1333	After surgical implantation into rabbit calvarial bone defects, new blood vessel formation was significantly higher with MK1-EPS composite bone granules than with granules alone, and new bone formation increased significantly.
27357535	3	58	dep	HUVEC	434:438	arg1	formation					485:493	vascular tube formation	471:493	vascular tube formation	471:493	MK1-EPS significantly increased human umbilical vein endothelial cell (HUVEC) proliferation, migration, and vascular tube formation.
27357535	3	58	dep	HUVEC	434:438	arg1	migration					456:464	migration	456:464	migration	456:464	MK1-EPS significantly increased human umbilical vein endothelial cell (HUVEC) proliferation, migration, and vascular tube formation.
27357535	3	58	dep	HUVEC	434:438	arg1	proliferation					441:453	proliferation	441:453	proliferation	441:453	MK1-EPS significantly increased human umbilical vein endothelial cell (HUVEC) proliferation, migration, and vascular tube formation.
27357535	3	58	dep	HUVEC	434:438	arg1	HUVEC					434:438	human umbilical vein endothelial cell (HUVEC)	395:439	human umbilical vein endothelial cell (HUVEC) proliferation, migration, and vascular tube formation	395:493	MK1-EPS significantly increased human umbilical vein endothelial cell (HUVEC) proliferation, migration, and vascular tube formation.
27357535	2	59	theme	exopolysaccharide	254:270	arg1	properties					237:246	the angiogenic properties	222:246	the angiogenic properties of an exopolysaccharide (EPS) secreted by MK1 (MK1-EPS), a novel bacterial strain isolated from Neungee mushrooms	222:360	Here, we evaluated the angiogenic properties of an exopolysaccharide (EPS) secreted by MK1 (MK1-EPS), a novel bacterial strain isolated from Neungee mushrooms.
27357535	5	60	theme	B	855:855	arg1	phosphorylation					750:764	phosphorylation	750:764	phosphorylation of signal transducer and activator of transcription 3 (STAT3), but not of protein kinase B (AKT),	750:862	In addition, the expression of p21 and intercellular adhesion molecule 1 (ICAM1), and phosphorylation of signal transducer and activator of transcription 3 (STAT3), but not of protein kinase B (AKT), were increased.
27357535	5	60	theme	B	855:855	arg1	expression					681:690	the expression	677:690	the expression of p21 and intercellular adhesion molecule 1 (ICAM1)	677:743	In addition, the expression of p21 and intercellular adhesion molecule 1 (ICAM1), and phosphorylation of signal transducer and activator of transcription 3 (STAT3), but not of protein kinase B (AKT), were increased.
27357535	4	61	theme	kinase	596:601	arg1	phosphorylation					517:531	the phosphorylation	513:531	the phosphorylation	513:531	MK1-EPS enhanced the phosphorylation of extracellular signal-related kinase (ERK), c-Jun N-terminal kinase (JNK), and p38, which are mitogen-activated protein kinases.
27357535	4	61	theme	kinase	596:601	arg1	kinases					655:661	mitogen-activated protein kinases	629:661	mitogen-activated protein kinases	629:661	MK1-EPS enhanced the phosphorylation of extracellular signal-related kinase (ERK), c-Jun N-terminal kinase (JNK), and p38, which are mitogen-activated protein kinases.
27357535	7	62	theme	new	1316:1318	arg1	formation					1325:1333	new bone formation	1316:1333	new bone formation	1316:1333	After surgical implantation into rabbit calvarial bone defects, new blood vessel formation was significantly higher with MK1-EPS composite bone granules than with granules alone, and new bone formation increased significantly.
27357535	8	63	theme	regeneration	1441:1452	arg1	agent					1454:1458	a bone regeneration agent	1434:1458	a bone regeneration agent	1434:1458	Therefore, MK1-EPS induces angiogenesis and may have potential for use as a bone regeneration agent in bone tissue engineering applications.
27357535	6	64	theme	p21	1091:1093	arg1	expression					1077:1086	MKI-EPS-induced expression	1061:1086	MKI-EPS-induced expression of p21 and ICAM1	1061:1103	Specific inhibitors of p38 (SB203580), ERK (PD98059), and JNK (SP600125) inhibited MK1-EPS-induced HUVEC proliferation, tube formation, and cell migration, and partially attenuated MKI-EPS-induced expression of p21 and ICAM1, and STAT3 phosphorylation.
27357535	6	64	theme	p21	1091:1093	arg1	phosphorylation					1116:1130	STAT3 phosphorylation	1110:1130	STAT3 phosphorylation	1110:1130	Specific inhibitors of p38 (SB203580), ERK (PD98059), and JNK (SP600125) inhibited MK1-EPS-induced HUVEC proliferation, tube formation, and cell migration, and partially attenuated MKI-EPS-induced expression of p21 and ICAM1, and STAT3 phosphorylation.
27357535	1	65	theme	essential	123:131	arg1	step					147:150	an essential physiological step	120:150	an essential physiological step in wound healing and other regenerative processes	120:200	Angiogenesis is an essential physiological step in wound healing and other regenerative processes.
27357535	1	65	theme	essential	123:131	arg1	Angiogenesis					104:115	Angiogenesis	104:115	Angiogenesis	104:115	Angiogenesis is an essential physiological step in wound healing and other regenerative processes.
27357535	6	66	theme	Specific	880:887	arg1	inhibitors					889:898	Specific inhibitors	880:898	Specific inhibitors of p38 (SB203580), ERK (PD98059), and JNK (SP600125)	880:951	Specific inhibitors of p38 (SB203580), ERK (PD98059), and JNK (SP600125) inhibited MK1-EPS-induced HUVEC proliferation, tube formation, and cell migration, and partially attenuated MKI-EPS-induced expression of p21 and ICAM1, and STAT3 phosphorylation.
27357535	4	67	theme	N-terminal	585:594	arg1	kinase					596:601	c-Jun N-terminal kinase	579:601	c-Jun N-terminal kinase (JNK)	579:607	MK1-EPS enhanced the phosphorylation of extracellular signal-related kinase (ERK), c-Jun N-terminal kinase (JNK), and p38, which are mitogen-activated protein kinases.
27357535	4	67	theme	N-terminal	585:594	arg1	JNK					604:606	JNK	604:606	JNK	604:606	MK1-EPS enhanced the phosphorylation of extracellular signal-related kinase (ERK), c-Jun N-terminal kinase (JNK), and p38, which are mitogen-activated protein kinases.
27357535	5	68	theme	adhesion	717:724	arg1	molecule					726:733	intercellular adhesion molecule 1	703:735	intercellular adhesion molecule 1 (ICAM1)	703:743	In addition, the expression of p21 and intercellular adhesion molecule 1 (ICAM1), and phosphorylation of signal transducer and activator of transcription 3 (STAT3), but not of protein kinase B (AKT), were increased.
27357535	5	68	theme	adhesion	717:724	arg1	ICAM1					738:742	ICAM1	738:742	ICAM1	738:742	In addition, the expression of p21 and intercellular adhesion molecule 1 (ICAM1), and phosphorylation of signal transducer and activator of transcription 3 (STAT3), but not of protein kinase B (AKT), were increased.
27357535	6	69	theme	HUVEC	979:983	arg1	proliferation					985:997	MK1-EPS-induced HUVEC proliferation	963:997	MK1-EPS-induced HUVEC proliferation	963:997	Specific inhibitors of p38 (SB203580), ERK (PD98059), and JNK (SP600125) inhibited MK1-EPS-induced HUVEC proliferation, tube formation, and cell migration, and partially attenuated MKI-EPS-induced expression of p21 and ICAM1, and STAT3 phosphorylation.
27357535	1	70	theme	physiological	133:145	arg1	step					147:150	an essential physiological step	120:150	an essential physiological step in wound healing and other regenerative processes	120:200	Angiogenesis is an essential physiological step in wound healing and other regenerative processes.
27357535	1	70	theme	physiological	133:145	arg1	Angiogenesis					104:115	Angiogenesis	104:115	Angiogenesis	104:115	Angiogenesis is an essential physiological step in wound healing and other regenerative processes.
27357535	0	71	theme	exopolysaccharide	48:64	arg1	potency					29:35	the angiogenic potency	14:35	the angiogenic potency of a novel exopolysaccharide	14:64	Evaluation of the angiogenic potency of a novel exopolysaccharide produced by the MK1 bacterial strain.
27357535	2	72	attach	isolated	330:337	arg2	strain					323:328	a novel bacterial strain	305:328	a novel bacterial strain isolated from Neungee mushrooms	305:360	Here, we evaluated the angiogenic properties of an exopolysaccharide (EPS) secreted by MK1 (MK1-EPS), a novel bacterial strain isolated from Neungee mushrooms.
27357535	2	72	attach	isolated	330:337	arg2	MK1					290:292	MK1	290:292	MK1 (MK1-EPS)	290:302	Here, we evaluated the angiogenic properties of an exopolysaccharide (EPS) secreted by MK1 (MK1-EPS), a novel bacterial strain isolated from Neungee mushrooms.
27357535	2	72	attach	isolated	330:337	arg1	mushrooms					352:360	Neungee mushrooms	344:360	Neungee mushrooms	344:360	Here, we evaluated the angiogenic properties of an exopolysaccharide (EPS) secreted by MK1 (MK1-EPS), a novel bacterial strain isolated from Neungee mushrooms.
27357535	7	73	theme	calvarial	1173:1181	arg1	defects					1188:1194	rabbit calvarial bone defects	1166:1194	rabbit calvarial bone defects	1166:1194	After surgical implantation into rabbit calvarial bone defects, new blood vessel formation was significantly higher with MK1-EPS composite bone granules than with granules alone, and new bone formation increased significantly.
27357535	4	74	theme	protein	647:653	arg1	phosphorylation					517:531	the phosphorylation	513:531	the phosphorylation	513:531	MK1-EPS enhanced the phosphorylation of extracellular signal-related kinase (ERK), c-Jun N-terminal kinase (JNK), and p38, which are mitogen-activated protein kinases.
27357535	4	74	theme	protein	647:653	arg1	kinases					655:661	mitogen-activated protein kinases	629:661	mitogen-activated protein kinases	629:661	MK1-EPS enhanced the phosphorylation of extracellular signal-related kinase (ERK), c-Jun N-terminal kinase (JNK), and p38, which are mitogen-activated protein kinases.
27357535	5	75	theme	p21	695:697	arg1	phosphorylation					750:764	phosphorylation	750:764	phosphorylation of signal transducer and activator of transcription 3 (STAT3), but not of protein kinase B (AKT),	750:862	In addition, the expression of p21 and intercellular adhesion molecule 1 (ICAM1), and phosphorylation of signal transducer and activator of transcription 3 (STAT3), but not of protein kinase B (AKT), were increased.
27357535	5	75	theme	p21	695:697	arg1	expression					681:690	the expression	677:690	the expression of p21 and intercellular adhesion molecule 1 (ICAM1)	677:743	In addition, the expression of p21 and intercellular adhesion molecule 1 (ICAM1), and phosphorylation of signal transducer and activator of transcription 3 (STAT3), but not of protein kinase B (AKT), were increased.
25548777	2	0	theme	castor	485:490	arg1	D					496:496	castor oil D	485:496	castor oil D (15-20% w/w)	485:509	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	2	0	theme	castor	485:490	arg1	 w/w					505:508	15-20% w/w	499:508	15-20% w/w	499:508	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	2	1	theme	compositions	331:342	arg1	influence					309:317	the influence	305:317	the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap	305:625	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	1	2	theme	corneum	231:237	arg1	application					239:249	stratum corneum application	223:249	stratum corneum application	223:249	Okara, soybean waste from tofu and soymilk production, was utilised as a natural antioxidant in soap formulation for stratum corneum application.
25548777	2	3	from	influence	309:317	arg1	hardness					606:613	the response hardness	593:613	the response hardness of the soap	593:625	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	6	4	theme	based	1189:1193	arg1	soap					1195:1198	okara based soap	1183:1198	okara based soap	1183:1198	Depending on the desirable level of those six variables, creation of okara based soap with desirable properties better than those of commercial ones is possible.
25548777	2	5	theme	%	556:556	arg1	 w/w					557:560	2-7% w/w	553:560	2-7% w/w	553:560	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	2	5	theme	%	556:556	arg1	F					550:550	okara F	544:550	okara F (2-7% w/w)	544:561	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	2	6	theme	D-optimal	252:260	arg1	design					270:275	D-optimal mixture design	252:275	D-optimal mixture design	252:275	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	3	7	theme	design	795:800	arg1	factors					802:808	the six design factors	787:808	the six design factors considered in this study	787:833	The experimental data were utilized to carry out analysis of variance (ANOVA) and to develop a polynomial regression model for okara soap hardness in terms of the six design factors considered in this study.
25548777	2	8	theme	oil	466:468	arg1	 w/w					478:481	6-10% w/w	473:481	6-10% w/w	473:481	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	2	8	theme	oil	466:468	arg1	C					470:470	palm oil C	461:470	palm oil C (6-10% w/w)	461:482	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	6	9	with	creation	1171:1178	arg1	properties					1215:1224	desirable properties	1205:1224	desirable properties better than those of commercial ones	1205:1261	Depending on the desirable level of those six variables, creation of okara based soap with desirable properties better than those of commercial ones is possible.
25548777	2	10	contain	containing	358:367	arg2	oils					394:397	oils	394:397	oils	394:397	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	2	10	contain	containing	358:367	arg2	acid					385:388	different fatty acid	369:388	different fatty acid	369:388	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	2	10	contain	containing	358:367	arg1	soap					353:356	okara soap	347:356	okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process	347:588	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	4	11	theme	best	862:865	arg1	mixture					867:873	the best mixture	858:873	the best mixture	858:873	Results revealed that the best mixture was the formulation that included 26.537% A, 19.999% B, 9.998% C, 16.241% D, 7.633% E, and 7.000% F.
25548777	4	11	theme	best	862:865	arg1	formulation					883:893	the formulation	879:893	the formulation that included 26.537% A, 19.999% B, 9.998% C, 16.241% D, 7.633% E, and 7.000% F	879:973	Results revealed that the best mixture was the formulation that included 26.537% A, 19.999% B, 9.998% C, 16.241% D, 7.633% E, and 7.000% F.
25548777	2	12	theme	%	477:477	arg1	 w/w					478:481	6-10% w/w	473:481	6-10% w/w	473:481	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	2	12	theme	%	477:477	arg1	C					470:470	palm oil C	461:470	palm oil C (6-10% w/w)	461:482	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	3	13	theme	experimental	632:643	arg1	data					645:648	The experimental data	628:648	The experimental data	628:648	The experimental data were utilized to carry out analysis of variance (ANOVA) and to develop a polynomial regression model for okara soap hardness in terms of the six design factors considered in this study.
25548777	4	14	theme	%	936:936	arg1	C					938:938	9.998% C	931:938	9.998% C	931:938	Results revealed that the best mixture was the formulation that included 26.537% A, 19.999% B, 9.998% C, 16.241% D, 7.633% E, and 7.000% F.
25548777	4	15	theme	%	926:926	arg1	B					928:928	19.999% B	920:928	19.999% B	920:928	Results revealed that the best mixture was the formulation that included 26.537% A, 19.999% B, 9.998% C, 16.241% D, 7.633% E, and 7.000% F.
25548777	2	16	theme	okara	544:548	arg1	 w/w					557:560	2-7% w/w	553:560	2-7% w/w	553:560	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	2	16	theme	okara	544:548	arg1	F					550:550	okara F	544:550	okara F (2-7% w/w)	544:561	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	0	17	theme	okara	50:54	arg1	formulation					61:71	okara soap formulation	50:71	okara soap formulation for stratum corneum application	50:103	The use of D-optimal mixture design in optimising okara soap formulation for stratum corneum application.
25548777	1	18	from	production	149:158	arg1	Okara					106:110	Okara	106:110	Okara	106:110	Okara, soybean waste from tofu and soymilk production, was utilised as a natural antioxidant in soap formulation for stratum corneum application.
25548777	1	18	from	production	149:158	arg1	waste					121:125	soybean waste	113:125	soybean waste from tofu and soymilk production	113:158	Okara, soybean waste from tofu and soymilk production, was utilised as a natural antioxidant in soap formulation for stratum corneum application.
25548777	2	19	theme	olive	435:439	arg1	B					445:445	olive oil B	435:445	olive oil B (15-20% w/w)	435:458	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	2	19	theme	olive	435:439	arg1	 w/w					454:457	15-20% w/w	448:457	15-20% w/w	448:457	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	4	20	theme	%	915:915	arg1	A					917:917	A	917:917	A	917:917	Results revealed that the best mixture was the formulation that included 26.537% A, 19.999% B, 9.998% C, 16.241% D, 7.633% E, and 7.000% F.
25548777	1	21	theme	soymilk	141:147	arg1	production					149:158	soymilk production	141:158	soymilk production	141:158	Okara, soybean waste from tofu and soymilk production, was utilised as a natural antioxidant in soap formulation for stratum corneum application.
25548777	0	22	theme	stratum	77:83	arg1	corneum					85:91	stratum corneum	77:91	stratum corneum application	77:103	The use of D-optimal mixture design in optimising okara soap formulation for stratum corneum application.
25548777	5	23	theme	soap	1108:1111	arg1	hardness					1096:1103	the hardness	1092:1103	the hardness of soap	1092:1111	The results proved that the difference in the level of fatty acid and oils in the formulation significantly affects the hardness of soap.
25548777	4	24	theme	%	947:947	arg1	D					949:949	16.241% D	941:949	16.241% D	941:949	Results revealed that the best mixture was the formulation that included 26.537% A, 19.999% B, 9.998% C, 16.241% D, 7.633% E, and 7.000% F.
25548777	2	25	theme	different	369:377	arg1	acid					385:388	different fatty acid	369:388	different fatty acid	369:388	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	2	26	theme	oil	415:417	arg1	A					419:419	virgin coconut oil A	400:419	virgin coconut oil A (24-28% w/w)	400:432	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	2	26	theme	oil	415:417	arg1	 w/w					428:431	24-28% w/w	422:431	24-28% w/w	422:431	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	2	27	theme	cocoa	512:516	arg1	 w/w					533:536	6-10% w/w	528:536	6-10% w/w	528:536	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	2	27	theme	cocoa	512:516	arg1	E					525:525	cocoa butter E	512:525	cocoa butter E (6-10% w/w)	512:537	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	2	28	theme	virgin	400:405	arg1	A					419:419	virgin coconut oil A	400:419	virgin coconut oil A (24-28% w/w)	400:432	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	2	28	theme	virgin	400:405	arg1	 w/w					428:431	24-28% w/w	422:431	24-28% w/w	422:431	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	0	29	theme	mixture	21:27	arg1	design					29:34	D-optimal mixture design	11:34	D-optimal mixture design	11:34	The use of D-optimal mixture design in optimising okara soap formulation for stratum corneum application.
25548777	2	30	theme	%	427:427	arg1	A					419:419	virgin coconut oil A	400:419	virgin coconut oil A (24-28% w/w)	400:432	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	2	30	theme	%	427:427	arg1	 w/w					428:431	24-28% w/w	422:431	24-28% w/w	422:431	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	4	31	theme	7.000	966:970	arg1	%					971:971	%	971:971	%	971:971	Results revealed that the best mixture was the formulation that included 26.537% A, 19.999% B, 9.998% C, 16.241% D, 7.633% E, and 7.000% F.
25548777	5	32	theme	acid	1037:1040	arg1	level					1022:1026	the level	1018:1026	the level of fatty acid and oils in the formulation	1018:1068	The results proved that the difference in the level of fatty acid and oils in the formulation significantly affects the hardness of soap.
25548777	2	33	theme	main	326:329	arg1	compositions					331:342	the main compositions	322:342	the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process	322:588	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	2	34	theme	response	597:604	arg1	hardness					606:613	the response hardness	593:613	the response hardness of the soap	593:625	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	5	35	theme	oils	1046:1049	arg1	level					1022:1026	the level	1018:1026	the level of fatty acid and oils in the formulation	1018:1068	The results proved that the difference in the level of fatty acid and oils in the formulation significantly affects the hardness of soap.
25548777	4	36	theme	%	957:957	arg1	E					959:959	7.633% E	952:959	7.633% E	952:959	Results revealed that the best mixture was the formulation that included 26.537% A, 19.999% B, 9.998% C, 16.241% D, 7.633% E, and 7.000% F.
25548777	2	37	dep	acid	385:388	arg1	 w/w					505:508	15-20% w/w	499:508	15-20% w/w	499:508	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	2	37	dep	acid	385:388	arg1	C					470:470	palm oil C	461:470	palm oil C (6-10% w/w)	461:482	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	2	37	dep	acid	385:388	arg1	F					550:550	okara F	544:550	okara F (2-7% w/w)	544:561	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	2	37	dep	acid	385:388	arg1	A					419:419	virgin coconut oil A	400:419	virgin coconut oil A (24-28% w/w)	400:432	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	2	37	dep	acid	385:388	arg1	 w/w					428:431	24-28% w/w	422:431	24-28% w/w	422:431	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	2	37	dep	acid	385:388	arg1	 w/w					454:457	15-20% w/w	448:457	15-20% w/w	448:457	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	2	37	dep	acid	385:388	arg1	 w/w					533:536	6-10% w/w	528:536	6-10% w/w	528:536	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	2	37	dep	acid	385:388	arg1	D					496:496	castor oil D	485:496	castor oil D (15-20% w/w)	485:509	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	2	37	dep	acid	385:388	arg1	B					445:445	olive oil B	435:445	olive oil B (15-20% w/w)	435:458	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	2	37	dep	acid	385:388	arg1	 w/w					478:481	6-10% w/w	473:481	6-10% w/w	473:481	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	2	37	dep	acid	385:388	arg1	 w/w					557:560	2-7% w/w	553:560	2-7% w/w	553:560	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	2	37	dep	acid	385:388	arg1	E					525:525	cocoa butter E	512:525	cocoa butter E (6-10% w/w)	512:537	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	2	38	theme	soap	353:356	arg1	compositions					331:342	the main compositions	322:342	the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process	322:588	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	3	39	theme	regression	734:743	arg1	model					745:749	a polynomial regression model	721:749	a polynomial regression model for okara soap hardness in terms of the six design factors considered in this study	721:833	The experimental data were utilized to carry out analysis of variance (ANOVA) and to develop a polynomial regression model for okara soap hardness in terms of the six design factors considered in this study.
25548777	3	40	from	hardness	766:773	arg1	terms					778:782	terms	778:782	terms of the six design factors considered in this study	778:833	The experimental data were utilized to carry out analysis of variance (ANOVA) and to develop a polynomial regression model for okara soap hardness in terms of the six design factors considered in this study.
25548777	2	41	theme	soap	622:625	arg1	hardness					606:613	the response hardness	593:613	the response hardness of the soap	593:625	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	1	42	theme	stratum	223:229	arg1	corneum					231:237	stratum corneum	223:237	stratum corneum application	223:249	Okara, soybean waste from tofu and soymilk production, was utilised as a natural antioxidant in soap formulation for stratum corneum application.
25548777	3	43	theme	soap	761:764	arg1	hardness					766:773	okara soap hardness	755:773	okara soap hardness in terms of the six design factors considered in this study	755:833	The experimental data were utilized to carry out analysis of variance (ANOVA) and to develop a polynomial regression model for okara soap hardness in terms of the six design factors considered in this study.
25548777	2	44	theme	mixture	262:268	arg1	design					270:275	D-optimal mixture design	252:275	D-optimal mixture design	252:275	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	6	45	theme	okara	1183:1187	arg1	soap					1195:1198	okara based soap	1183:1198	okara based soap	1183:1198	Depending on the desirable level of those six variables, creation of okara based soap with desirable properties better than those of commercial ones is possible.
25548777	2	46	theme	oil	492:494	arg1	D					496:496	castor oil D	485:496	castor oil D (15-20% w/w)	485:509	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	2	46	theme	oil	492:494	arg1	 w/w					505:508	15-20% w/w	499:508	15-20% w/w	499:508	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	2	47	theme	saponification	567:580	arg1	process					582:588	saponification process	567:588	saponification process	567:588	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	6	48	theme	variables	1160:1168	arg1	level					1141:1145	the desirable level	1127:1145	the desirable level of those six variables	1127:1168	Depending on the desirable level of those six variables, creation of okara based soap with desirable properties better than those of commercial ones is possible.
25548777	2	49	theme	palm	461:464	arg1	 w/w					478:481	6-10% w/w	473:481	6-10% w/w	473:481	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	2	49	theme	palm	461:464	arg1	C					470:470	palm oil C	461:470	palm oil C (6-10% w/w)	461:482	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	1	50	theme	natural	179:185	arg1	Okara					106:110	Okara	106:110	Okara	106:110	Okara, soybean waste from tofu and soymilk production, was utilised as a natural antioxidant in soap formulation for stratum corneum application.
25548777	1	50	theme	natural	179:185	arg1	antioxidant					187:197	a natural antioxidant	177:197	a natural antioxidant in soap formulation for stratum corneum application	177:249	Okara, soybean waste from tofu and soymilk production, was utilised as a natural antioxidant in soap formulation for stratum corneum application.
25548777	4	51	theme	19.999	920:925	arg1	%					926:926	%	926:926	%	926:926	Results revealed that the best mixture was the formulation that included 26.537% A, 19.999% B, 9.998% C, 16.241% D, 7.633% E, and 7.000% F.
25548777	3	52	used	utilized	655:662	arg2	data					645:648	The experimental data	628:648	The experimental data	628:648	The experimental data were utilized to carry out analysis of variance (ANOVA) and to develop a polynomial regression model for okara soap hardness in terms of the six design factors considered in this study.
25548777	1	53	from	antioxidant	187:197	arg1	formulation					207:217	soap formulation	202:217	soap formulation	202:217	Okara, soybean waste from tofu and soymilk production, was utilised as a natural antioxidant in soap formulation for stratum corneum application.
25548777	2	54	theme	%	532:532	arg1	 w/w					533:536	6-10% w/w	528:536	6-10% w/w	528:536	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	2	54	theme	%	532:532	arg1	E					525:525	cocoa butter E	512:525	cocoa butter E (6-10% w/w)	512:537	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	6	55	theme	better	1226:1231	arg1	properties					1215:1224	desirable properties	1205:1224	desirable properties better than those of commercial ones	1205:1261	Depending on the desirable level of those six variables, creation of okara based soap with desirable properties better than those of commercial ones is possible.
25548777	1	56	theme	soybean	113:119	arg1	Okara					106:110	Okara	106:110	Okara	106:110	Okara, soybean waste from tofu and soymilk production, was utilised as a natural antioxidant in soap formulation for stratum corneum application.
25548777	1	56	theme	soybean	113:119	arg1	waste					121:125	soybean waste	113:125	soybean waste from tofu and soymilk production	113:158	Okara, soybean waste from tofu and soymilk production, was utilised as a natural antioxidant in soap formulation for stratum corneum application.
25548777	6	57	theme	commercial	1247:1256	arg1	ones					1258:1261	commercial ones	1247:1261	commercial ones	1247:1261	Depending on the desirable level of those six variables, creation of okara based soap with desirable properties better than those of commercial ones is possible.
25548777	5	58	from	difference	1004:1013	arg1	level					1022:1026	the level	1018:1026	the level of fatty acid and oils in the formulation	1018:1068	The results proved that the difference in the level of fatty acid and oils in the formulation significantly affects the hardness of soap.
25548777	4	59	theme	26.537	909:914	arg1	%					915:915	%	915:915	%	915:915	Results revealed that the best mixture was the formulation that included 26.537% A, 19.999% B, 9.998% C, 16.241% D, 7.633% E, and 7.000% F.
25548777	0	60	theme	soap	56:59	arg1	formulation					61:71	okara soap formulation	50:71	okara soap formulation for stratum corneum application	50:103	The use of D-optimal mixture design in optimising okara soap formulation for stratum corneum application.
25548777	6	61	theme	desirable	1205:1213	arg1	properties					1215:1224	desirable properties	1205:1224	desirable properties better than those of commercial ones	1205:1261	Depending on the desirable level of those six variables, creation of okara based soap with desirable properties better than those of commercial ones is possible.
25548777	2	62	theme	oil	441:443	arg1	B					445:445	olive oil B	435:445	olive oil B (15-20% w/w)	435:458	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	2	62	theme	oil	441:443	arg1	 w/w					454:457	15-20% w/w	448:457	15-20% w/w	448:457	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	6	63	theme	soap	1195:1198	arg1	creation					1171:1178	creation	1171:1178	creation of okara based soap with desirable properties better than those of commercial ones	1171:1261	Depending on the desirable level of those six variables, creation of okara based soap with desirable properties better than those of commercial ones is possible.
25548777	4	64	theme	16.241	941:946	arg1	%					947:947	%	947:947	%	947:947	Results revealed that the best mixture was the formulation that included 26.537% A, 19.999% B, 9.998% C, 16.241% D, 7.633% E, and 7.000% F.
25548777	0	65	theme	corneum	85:91	arg1	application					93:103	stratum corneum application	77:103	stratum corneum application	77:103	The use of D-optimal mixture design in optimising okara soap formulation for stratum corneum application.
25548777	3	66	theme	factors	802:808	arg1	terms					778:782	terms	778:782	terms of the six design factors considered in this study	778:833	The experimental data were utilized to carry out analysis of variance (ANOVA) and to develop a polynomial regression model for okara soap hardness in terms of the six design factors considered in this study.
25548777	2	67	theme	%	453:453	arg1	B					445:445	olive oil B	435:445	olive oil B (15-20% w/w)	435:458	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	2	67	theme	%	453:453	arg1	 w/w					454:457	15-20% w/w	448:457	15-20% w/w	448:457	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	1	68	from	tofu	132:135	arg1	Okara					106:110	Okara	106:110	Okara	106:110	Okara, soybean waste from tofu and soymilk production, was utilised as a natural antioxidant in soap formulation for stratum corneum application.
25548777	1	68	from	tofu	132:135	arg1	waste					121:125	soybean waste	113:125	soybean waste from tofu and soymilk production	113:158	Okara, soybean waste from tofu and soymilk production, was utilised as a natural antioxidant in soap formulation for stratum corneum application.
25548777	3	69	theme	variance	689:696	arg1	analysis					677:684	analysis	677:684	analysis of variance (ANOVA)	677:704	The experimental data were utilized to carry out analysis of variance (ANOVA) and to develop a polynomial regression model for okara soap hardness in terms of the six design factors considered in this study.
25548777	5	70	from	level	1022:1026	arg1	formulation					1058:1068	the formulation	1054:1068	the formulation	1054:1068	The results proved that the difference in the level of fatty acid and oils in the formulation significantly affects the hardness of soap.
25548777	4	71	theme	9.998	931:935	arg1	%					936:936	%	936:936	%	936:936	Results revealed that the best mixture was the formulation that included 26.537% A, 19.999% B, 9.998% C, 16.241% D, 7.633% E, and 7.000% F.
25548777	2	72	theme	fatty	379:383	arg1	acid					385:388	different fatty acid	369:388	different fatty acid	369:388	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	2	73	theme	butter	518:523	arg1	 w/w					533:536	6-10% w/w	528:536	6-10% w/w	528:536	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	2	73	theme	butter	518:523	arg1	E					525:525	cocoa butter E	512:525	cocoa butter E (6-10% w/w)	512:537	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	0	74	theme	D-optimal	11:19	arg1	design					29:34	D-optimal mixture design	11:34	D-optimal mixture design	11:34	The use of D-optimal mixture design in optimising okara soap formulation for stratum corneum application.
25548777	2	75	theme	%	504:504	arg1	D					496:496	castor oil D	485:496	castor oil D (15-20% w/w)	485:509	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	2	75	theme	%	504:504	arg1	 w/w					505:508	15-20% w/w	499:508	15-20% w/w	499:508	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	2	76	theme	coconut	407:413	arg1	A					419:419	virgin coconut oil A	400:419	virgin coconut oil A (24-28% w/w)	400:432	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	2	76	theme	coconut	407:413	arg1	 w/w					428:431	24-28% w/w	422:431	24-28% w/w	422:431	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
25548777	0	77	theme	design	29:34	arg1	use					4:6	The use	0:6	The use of D-optimal mixture design in optimising okara soap formulation for stratum corneum application.	0:104	The use of D-optimal mixture design in optimising okara soap formulation for stratum corneum application.
25548777	4	78	theme	%	971:971	arg1	F					973:973	7.000% F	966:973	7.000% F	966:973	Results revealed that the best mixture was the formulation that included 26.537% A, 19.999% B, 9.998% C, 16.241% D, 7.633% E, and 7.000% F.
25548777	5	79	theme	fatty	1031:1035	arg1	acid					1037:1040	fatty acid	1031:1040	fatty acid	1031:1040	The results proved that the difference in the level of fatty acid and oils in the formulation significantly affects the hardness of soap.
25548777	3	80	theme	polynomial	723:732	arg1	model					745:749	a polynomial regression model	721:749	a polynomial regression model for okara soap hardness in terms of the six design factors considered in this study	721:833	The experimental data were utilized to carry out analysis of variance (ANOVA) and to develop a polynomial regression model for okara soap hardness in terms of the six design factors considered in this study.
25548777	4	81	theme	7.633	952:956	arg1	%					957:957	%	957:957	%	957:957	Results revealed that the best mixture was the formulation that included 26.537% A, 19.999% B, 9.998% C, 16.241% D, 7.633% E, and 7.000% F.
25548777	6	82	theme	desirable	1131:1139	arg1	level					1141:1145	the desirable level	1127:1145	the desirable level of those six variables	1127:1168	Depending on the desirable level of those six variables, creation of okara based soap with desirable properties better than those of commercial ones is possible.
25548777	1	83	theme	soap	202:205	arg1	formulation					207:217	soap formulation	202:217	soap formulation	202:217	Okara, soybean waste from tofu and soymilk production, was utilised as a natural antioxidant in soap formulation for stratum corneum application.
25548777	3	84	theme	okara	755:759	arg1	hardness					766:773	okara soap hardness	755:773	okara soap hardness in terms of the six design factors considered in this study	755:833	The experimental data were utilized to carry out analysis of variance (ANOVA) and to develop a polynomial regression model for okara soap hardness in terms of the six design factors considered in this study.
25548777	2	85	theme	okara	347:351	arg1	soap					353:356	okara soap	347:356	okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process	347:588	D-optimal mixture design was employed to investigate the influence of the main compositions of okara soap containing different fatty acid and oils (virgin coconut oil A (24-28% w/w), olive oil B (15-20% w/w), palm oil C (6-10% w/w), castor oil D (15-20% w/w), cocoa butter E (6-10% w/w), and okara F (2-7% w/w)) by saponification process on the response hardness of the soap.
27474595	6	0	from	additive	1109:1116	arg1	cosmetic					1136:1143	cosmetic	1136:1143	cosmetic	1136:1143	Overall, the results indicate that CNSP have attractive chemical, functional and biological properties, with a preliminary structural may have a backbone of branched 6-O-sulfated (1→4) galactosidic linkages, which can be considered in the future as alternative additive in various foods, cosmetic and pharmaceutical preparations.
27474595	6	0	from	additive	1109:1116	arg1	preparations					1164:1175	pharmaceutical preparations	1149:1175	pharmaceutical preparations	1149:1175	Overall, the results indicate that CNSP have attractive chemical, functional and biological properties, with a preliminary structural may have a backbone of branched 6-O-sulfated (1→4) galactosidic linkages, which can be considered in the future as alternative additive in various foods, cosmetic and pharmaceutical preparations.
27474595	6	0	from	additive	1109:1116	arg1	foods					1129:1133	various foods	1121:1133	various foods	1121:1133	Overall, the results indicate that CNSP have attractive chemical, functional and biological properties, with a preliminary structural may have a backbone of branched 6-O-sulfated (1→4) galactosidic linkages, which can be considered in the future as alternative additive in various foods, cosmetic and pharmaceutical preparations.
27474595	0	1	theme	polysaccharide	100:113	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and functional properties of antihypertensive Cymodocea nodosa sulfated polysaccharide.
27474595	0	1	theme	polysaccharide	100:113	arg1	properties					43:52	functional properties	32:52	functional properties	32:52	Structural characterization and functional properties of antihypertensive Cymodocea nodosa sulfated polysaccharide.
27474595	6	2	contain	have	888:891	arg1	CNSP					883:886	CNSP	883:886	CNSP	883:886	Overall, the results indicate that CNSP have attractive chemical, functional and biological properties, with a preliminary structural may have a backbone of branched 6-O-sulfated (1→4) galactosidic linkages, which can be considered in the future as alternative additive in various foods, cosmetic and pharmaceutical preparations.
27474595	6	2	contain	have	888:891	arg2	properties					940:949	attractive chemical, functional and biological properties	893:949	attractive chemical, functional and biological properties	893:949	Overall, the results indicate that CNSP have attractive chemical, functional and biological properties, with a preliminary structural may have a backbone of branched 6-O-sulfated (1→4) galactosidic linkages, which can be considered in the future as alternative additive in various foods, cosmetic and pharmaceutical preparations.
27474595	4	3	contain	had	582:584	arg1	CNSP					577:580	CNSP	577:580	CNSP	577:580	CNSP had an XRD pattern that was typical for a semi-crystalline polymer with homogeneous structure.
27474595	4	3	contain	had	582:584	arg2	typical					610:616	typical	610:616	typical	610:616	CNSP had an XRD pattern that was typical for a semi-crystalline polymer with homogeneous structure.
27474595	4	3	contain	had	582:584	arg2	pattern					593:599	an XRD pattern	586:599	an XRD pattern that was typical for a semi-crystalline polymer with homogeneous structure	586:674	CNSP had an XRD pattern that was typical for a semi-crystalline polymer with homogeneous structure.
27474595	6	4	theme	linkages	1046:1053	arg1	backbone					993:1000	a backbone	991:1000	a backbone of branched 6-O-sulfated (1→4) galactosidic linkages, which can be considered in the future as alternative additive in various foods, cosmetic and pharmaceutical preparations	991:1175	Overall, the results indicate that CNSP have attractive chemical, functional and biological properties, with a preliminary structural may have a backbone of branched 6-O-sulfated (1→4) galactosidic linkages, which can be considered in the future as alternative additive in various foods, cosmetic and pharmaceutical preparations.
27474595	4	5	with	polymer	641:647	arg1	structure					666:674	homogeneous structure	654:674	homogeneous structure	654:674	CNSP had an XRD pattern that was typical for a semi-crystalline polymer with homogeneous structure.
27474595	3	6	theme	sulfate	389:395	arg1	galactose					430:438	sulfate (23.17%), total sugars (54.90%), galactose	389:438	sulfate (23.17%), total sugars (54.90%), galactose (44.89%)	389:447	The CNSP consisted mainly of sulfate (23.17%), total sugars (54.90%), galactose (44.89%), mannose (17.30%), arabinose (12.05%), xylose (9.18%), maltose (1.07%) and uronic acid (11.03%) with low water activity (0.49).
27474595	3	6	theme	sulfate	389:395	arg1	%					446:446	44.89%	441:446	44.89%	441:446	The CNSP consisted mainly of sulfate (23.17%), total sugars (54.90%), galactose (44.89%), mannose (17.30%), arabinose (12.05%), xylose (9.18%), maltose (1.07%) and uronic acid (11.03%) with low water activity (0.49).
27474595	5	7	theme	hydrate	828:834	arg1	salt					836:839	N-hippuryl-His-Leu hydrate salt	809:839	N-hippuryl-His-Leu hydrate salt (HHL)	809:845	It also displayed an important anti-hypertensive activity (IC50=0.43mgml) with a dose-dependent manner using a synthetic substrate, N-hippuryl-His-Leu hydrate salt (HHL).
27474595	5	7	theme	hydrate	828:834	arg1	substrate					798:806	a synthetic substrate	786:806	a synthetic substrate	786:806	It also displayed an important anti-hypertensive activity (IC50=0.43mgml) with a dose-dependent manner using a synthetic substrate, N-hippuryl-His-Leu hydrate salt (HHL).
27474595	5	7	theme	hydrate	828:834	arg1	HHL					842:844	HHL	842:844	HHL	842:844	It also displayed an important anti-hypertensive activity (IC50=0.43mgml) with a dose-dependent manner using a synthetic substrate, N-hippuryl-His-Leu hydrate salt (HHL).
27474595	6	8	theme	functional	914:923	arg1	properties					940:949	attractive chemical, functional and biological properties	893:949	attractive chemical, functional and biological properties	893:949	Overall, the results indicate that CNSP have attractive chemical, functional and biological properties, with a preliminary structural may have a backbone of branched 6-O-sulfated (1→4) galactosidic linkages, which can be considered in the future as alternative additive in various foods, cosmetic and pharmaceutical preparations.
27474595	2	9	theme	antihypertensive	308:323	arg1	properties					325:334	functional and antihypertensive properties	293:334	functional and antihypertensive properties of this polysaccharide	293:357	This is the first report that indicates the chemical composition, structural characterization, functional and antihypertensive properties of this polysaccharide.
27474595	2	9	theme	antihypertensive	308:323	arg1	composition					251:261	the chemical composition	238:261	the chemical composition	238:261	This is the first report that indicates the chemical composition, structural characterization, functional and antihypertensive properties of this polysaccharide.
27474595	3	10	theme	total	407:411	arg1	sugars					413:418	total sugars	407:418	total sugars (54.90%)	407:427	The CNSP consisted mainly of sulfate (23.17%), total sugars (54.90%), galactose (44.89%), mannose (17.30%), arabinose (12.05%), xylose (9.18%), maltose (1.07%) and uronic acid (11.03%) with low water activity (0.49).
27474595	3	10	theme	total	407:411	arg1	%					426:426	54.90%	421:426	54.90%	421:426	The CNSP consisted mainly of sulfate (23.17%), total sugars (54.90%), galactose (44.89%), mannose (17.30%), arabinose (12.05%), xylose (9.18%), maltose (1.07%) and uronic acid (11.03%) with low water activity (0.49).
27474595	3	10	theme	total	407:411	arg1	sulfate					389:395	sulfate	389:395	sulfate (23.17%)	389:404	The CNSP consisted mainly of sulfate (23.17%), total sugars (54.90%), galactose (44.89%), mannose (17.30%), arabinose (12.05%), xylose (9.18%), maltose (1.07%) and uronic acid (11.03%) with low water activity (0.49).
27474595	5	11	theme	N-hippuryl-His-Leu	809:826	arg1	salt					836:839	N-hippuryl-His-Leu hydrate salt	809:839	N-hippuryl-His-Leu hydrate salt (HHL)	809:845	It also displayed an important anti-hypertensive activity (IC50=0.43mgml) with a dose-dependent manner using a synthetic substrate, N-hippuryl-His-Leu hydrate salt (HHL).
27474595	5	11	theme	N-hippuryl-His-Leu	809:826	arg1	substrate					798:806	a synthetic substrate	786:806	a synthetic substrate	786:806	It also displayed an important anti-hypertensive activity (IC50=0.43mgml) with a dose-dependent manner using a synthetic substrate, N-hippuryl-His-Leu hydrate salt (HHL).
27474595	5	11	theme	N-hippuryl-His-Leu	809:826	arg1	HHL					842:844	HHL	842:844	HHL	842:844	It also displayed an important anti-hypertensive activity (IC50=0.43mgml) with a dose-dependent manner using a synthetic substrate, N-hippuryl-His-Leu hydrate salt (HHL).
27474595	0	12	theme	Structural	0:9	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and functional properties of antihypertensive Cymodocea nodosa sulfated polysaccharide.
27474595	6	13	theme	various	1121:1127	arg1	foods					1129:1133	various foods	1121:1133	various foods	1121:1133	Overall, the results indicate that CNSP have attractive chemical, functional and biological properties, with a preliminary structural may have a backbone of branched 6-O-sulfated (1→4) galactosidic linkages, which can be considered in the future as alternative additive in various foods, cosmetic and pharmaceutical preparations.
27474595	1	14	theme	sulfated	118:125	arg1	polysaccharide					127:140	A sulfated polysaccharide	116:140	A sulfated polysaccharide	116:140	A sulfated polysaccharide was successfully isolated from Cymodocea nodosa (CNSP).
27474595	6	15	theme	biological	929:938	arg1	properties					940:949	attractive chemical, functional and biological properties	893:949	attractive chemical, functional and biological properties	893:949	Overall, the results indicate that CNSP have attractive chemical, functional and biological properties, with a preliminary structural may have a backbone of branched 6-O-sulfated (1→4) galactosidic linkages, which can be considered in the future as alternative additive in various foods, cosmetic and pharmaceutical preparations.
27474595	0	16	theme	functional	32:41	arg1	properties					43:52	functional properties	32:52	functional properties	32:52	Structural characterization and functional properties of antihypertensive Cymodocea nodosa sulfated polysaccharide.
27474595	2	17	theme	polysaccharide	344:357	arg1	properties					325:334	functional and antihypertensive properties	293:334	functional and antihypertensive properties of this polysaccharide	293:357	This is the first report that indicates the chemical composition, structural characterization, functional and antihypertensive properties of this polysaccharide.
27474595	2	17	theme	polysaccharide	344:357	arg1	composition					251:261	the chemical composition	238:261	the chemical composition	238:261	This is the first report that indicates the chemical composition, structural characterization, functional and antihypertensive properties of this polysaccharide.
27474595	4	18	theme	XRD	589:591	arg1	pattern					593:599	an XRD pattern	586:599	an XRD pattern that was typical for a semi-crystalline polymer with homogeneous structure	586:674	CNSP had an XRD pattern that was typical for a semi-crystalline polymer with homogeneous structure.
27474595	4	18	theme	XRD	589:591	arg1	typical					610:616	typical	610:616	typical	610:616	CNSP had an XRD pattern that was typical for a semi-crystalline polymer with homogeneous structure.
27474595	4	19	theme	semi-crystalline	624:639	arg1	polymer					641:647	a semi-crystalline polymer	622:647	a semi-crystalline polymer with homogeneous structure	622:674	CNSP had an XRD pattern that was typical for a semi-crystalline polymer with homogeneous structure.
27474595	6	20	contain	have	986:989	arg1	structural					971:980	structural	971:980	structural	971:980	Overall, the results indicate that CNSP have attractive chemical, functional and biological properties, with a preliminary structural may have a backbone of branched 6-O-sulfated (1→4) galactosidic linkages, which can be considered in the future as alternative additive in various foods, cosmetic and pharmaceutical preparations.
27474595	6	20	contain	have	986:989	arg2	backbone					993:1000	a backbone	991:1000	a backbone of branched 6-O-sulfated (1→4) galactosidic linkages, which can be considered in the future as alternative additive in various foods, cosmetic and pharmaceutical preparations	991:1175	Overall, the results indicate that CNSP have attractive chemical, functional and biological properties, with a preliminary structural may have a backbone of branched 6-O-sulfated (1→4) galactosidic linkages, which can be considered in the future as alternative additive in various foods, cosmetic and pharmaceutical preparations.
27474595	5	21	theme	important	698:706	arg1	IC50=0.43mgml					736:748	IC50=0.43mgml	736:748	IC50=0.43mgml	736:748	It also displayed an important anti-hypertensive activity (IC50=0.43mgml) with a dose-dependent manner using a synthetic substrate, N-hippuryl-His-Leu hydrate salt (HHL).
27474595	5	21	theme	important	698:706	arg1	activity					726:733	an important anti-hypertensive activity	695:733	an important anti-hypertensive activity (IC50=0.43mgml)	695:749	It also displayed an important anti-hypertensive activity (IC50=0.43mgml) with a dose-dependent manner using a synthetic substrate, N-hippuryl-His-Leu hydrate salt (HHL).
27474595	5	22	theme	anti-hypertensive	708:724	arg1	IC50=0.43mgml					736:748	IC50=0.43mgml	736:748	IC50=0.43mgml	736:748	It also displayed an important anti-hypertensive activity (IC50=0.43mgml) with a dose-dependent manner using a synthetic substrate, N-hippuryl-His-Leu hydrate salt (HHL).
27474595	5	22	theme	anti-hypertensive	708:724	arg1	activity					726:733	an important anti-hypertensive activity	695:733	an important anti-hypertensive activity (IC50=0.43mgml)	695:749	It also displayed an important anti-hypertensive activity (IC50=0.43mgml) with a dose-dependent manner using a synthetic substrate, N-hippuryl-His-Leu hydrate salt (HHL).
27474595	6	23	from	preparations	1164:1175	arg1	linkages					1046:1053	branched 6-O-sulfated (1→4) galactosidic linkages	1005:1053	branched 6-O-sulfated (1→4) galactosidic linkages	1005:1053	Overall, the results indicate that CNSP have attractive chemical, functional and biological properties, with a preliminary structural may have a backbone of branched 6-O-sulfated (1→4) galactosidic linkages, which can be considered in the future as alternative additive in various foods, cosmetic and pharmaceutical preparations.
27474595	6	23	from	preparations	1164:1175	arg1	additive					1109:1116	additive	1109:1116	additive	1109:1116	Overall, the results indicate that CNSP have attractive chemical, functional and biological properties, with a preliminary structural may have a backbone of branched 6-O-sulfated (1→4) galactosidic linkages, which can be considered in the future as alternative additive in various foods, cosmetic and pharmaceutical preparations.
27474595	6	24	theme	chemical	904:911	arg1	properties					940:949	attractive chemical, functional and biological properties	893:949	attractive chemical, functional and biological properties	893:949	Overall, the results indicate that CNSP have attractive chemical, functional and biological properties, with a preliminary structural may have a backbone of branched 6-O-sulfated (1→4) galactosidic linkages, which can be considered in the future as alternative additive in various foods, cosmetic and pharmaceutical preparations.
27474595	0	25	theme	Cymodocea	74:82	arg1	polysaccharide					100:113	antihypertensive Cymodocea nodosa sulfated polysaccharide	57:113	antihypertensive Cymodocea nodosa sulfated polysaccharide	57:113	Structural characterization and functional properties of antihypertensive Cymodocea nodosa sulfated polysaccharide.
27474595	2	26	theme	chemical	242:249	arg1	composition					251:261	the chemical composition	238:261	the chemical composition	238:261	This is the first report that indicates the chemical composition, structural characterization, functional and antihypertensive properties of this polysaccharide.
27474595	2	26	theme	chemical	242:249	arg1	properties					325:334	functional and antihypertensive properties	293:334	functional and antihypertensive properties of this polysaccharide	293:357	This is the first report that indicates the chemical composition, structural characterization, functional and antihypertensive properties of this polysaccharide.
27474595	2	26	theme	chemical	242:249	arg1	characterization					275:290	structural characterization	264:290	structural characterization	264:290	This is the first report that indicates the chemical composition, structural characterization, functional and antihypertensive properties of this polysaccharide.
27474595	0	27	theme	antihypertensive	57:72	arg1	polysaccharide					100:113	antihypertensive Cymodocea nodosa sulfated polysaccharide	57:113	antihypertensive Cymodocea nodosa sulfated polysaccharide	57:113	Structural characterization and functional properties of antihypertensive Cymodocea nodosa sulfated polysaccharide.
27474595	3	28	theme	low	550:552	arg1	0.49					570:573	0.49	570:573	0.49	570:573	The CNSP consisted mainly of sulfate (23.17%), total sugars (54.90%), galactose (44.89%), mannose (17.30%), arabinose (12.05%), xylose (9.18%), maltose (1.07%) and uronic acid (11.03%) with low water activity (0.49).
27474595	3	28	theme	low	550:552	arg1	activity					560:567	low water activity	550:567	low water activity (0.49)	550:574	The CNSP consisted mainly of sulfate (23.17%), total sugars (54.90%), galactose (44.89%), mannose (17.30%), arabinose (12.05%), xylose (9.18%), maltose (1.07%) and uronic acid (11.03%) with low water activity (0.49).
27474595	5	29	theme	synthetic	788:796	arg1	salt					836:839	N-hippuryl-His-Leu hydrate salt	809:839	N-hippuryl-His-Leu hydrate salt (HHL)	809:845	It also displayed an important anti-hypertensive activity (IC50=0.43mgml) with a dose-dependent manner using a synthetic substrate, N-hippuryl-His-Leu hydrate salt (HHL).
27474595	5	29	theme	synthetic	788:796	arg1	substrate					798:806	a synthetic substrate	786:806	a synthetic substrate	786:806	It also displayed an important anti-hypertensive activity (IC50=0.43mgml) with a dose-dependent manner using a synthetic substrate, N-hippuryl-His-Leu hydrate salt (HHL).
27474595	4	30	theme	homogeneous	654:664	arg1	structure					666:674	homogeneous structure	654:674	homogeneous structure	654:674	CNSP had an XRD pattern that was typical for a semi-crystalline polymer with homogeneous structure.
27474595	3	31	theme	water	554:558	arg1	0.49					570:573	0.49	570:573	0.49	570:573	The CNSP consisted mainly of sulfate (23.17%), total sugars (54.90%), galactose (44.89%), mannose (17.30%), arabinose (12.05%), xylose (9.18%), maltose (1.07%) and uronic acid (11.03%) with low water activity (0.49).
27474595	3	31	theme	water	554:558	arg1	activity					560:567	low water activity	550:567	low water activity (0.49)	550:574	The CNSP consisted mainly of sulfate (23.17%), total sugars (54.90%), galactose (44.89%), mannose (17.30%), arabinose (12.05%), xylose (9.18%), maltose (1.07%) and uronic acid (11.03%) with low water activity (0.49).
27474595	2	32	theme	functional	293:302	arg1	properties					325:334	functional and antihypertensive properties	293:334	functional and antihypertensive properties of this polysaccharide	293:357	This is the first report that indicates the chemical composition, structural characterization, functional and antihypertensive properties of this polysaccharide.
27474595	2	32	theme	functional	293:302	arg1	composition					251:261	the chemical composition	238:261	the chemical composition	238:261	This is the first report that indicates the chemical composition, structural characterization, functional and antihypertensive properties of this polysaccharide.
27474595	6	33	theme	6-O-sulfated	1014:1025	arg1	linkages					1046:1053	branched 6-O-sulfated (1→4) galactosidic linkages	1005:1053	branched 6-O-sulfated (1→4) galactosidic linkages	1005:1053	Overall, the results indicate that CNSP have attractive chemical, functional and biological properties, with a preliminary structural may have a backbone of branched 6-O-sulfated (1→4) galactosidic linkages, which can be considered in the future as alternative additive in various foods, cosmetic and pharmaceutical preparations.
27474595	6	33	theme	6-O-sulfated	1014:1025	arg1	additive					1109:1116	additive	1109:1116	additive	1109:1116	Overall, the results indicate that CNSP have attractive chemical, functional and biological properties, with a preliminary structural may have a backbone of branched 6-O-sulfated (1→4) galactosidic linkages, which can be considered in the future as alternative additive in various foods, cosmetic and pharmaceutical preparations.
27474595	6	34	theme	attractive	893:902	arg1	properties					940:949	attractive chemical, functional and biological properties	893:949	attractive chemical, functional and biological properties	893:949	Overall, the results indicate that CNSP have attractive chemical, functional and biological properties, with a preliminary structural may have a backbone of branched 6-O-sulfated (1→4) galactosidic linkages, which can be considered in the future as alternative additive in various foods, cosmetic and pharmaceutical preparations.
27474595	6	35	theme	pharmaceutical	1149:1162	arg1	preparations					1164:1175	pharmaceutical preparations	1149:1175	pharmaceutical preparations	1149:1175	Overall, the results indicate that CNSP have attractive chemical, functional and biological properties, with a preliminary structural may have a backbone of branched 6-O-sulfated (1→4) galactosidic linkages, which can be considered in the future as alternative additive in various foods, cosmetic and pharmaceutical preparations.
27474595	3	36	theme	uronic	524:529	arg1	acid					531:534	uronic acid	524:534	uronic acid (11.03%)	524:543	The CNSP consisted mainly of sulfate (23.17%), total sugars (54.90%), galactose (44.89%), mannose (17.30%), arabinose (12.05%), xylose (9.18%), maltose (1.07%) and uronic acid (11.03%) with low water activity (0.49).
27474595	3	36	theme	uronic	524:529	arg1	%					542:542	11.03%	537:542	11.03%	537:542	The CNSP consisted mainly of sulfate (23.17%), total sugars (54.90%), galactose (44.89%), mannose (17.30%), arabinose (12.05%), xylose (9.18%), maltose (1.07%) and uronic acid (11.03%) with low water activity (0.49).
27474595	1	37	theme	Cymodocea	173:181	arg1	CNSP					191:194	CNSP	191:194	CNSP	191:194	A sulfated polysaccharide was successfully isolated from Cymodocea nodosa (CNSP).
27474595	1	37	theme	Cymodocea	173:181	arg1	nodosa					183:188	Cymodocea nodosa	173:188	Cymodocea nodosa (CNSP)	173:195	A sulfated polysaccharide was successfully isolated from Cymodocea nodosa (CNSP).
27474595	6	38	from	foods	1129:1133	arg1	linkages					1046:1053	branched 6-O-sulfated (1→4) galactosidic linkages	1005:1053	branched 6-O-sulfated (1→4) galactosidic linkages	1005:1053	Overall, the results indicate that CNSP have attractive chemical, functional and biological properties, with a preliminary structural may have a backbone of branched 6-O-sulfated (1→4) galactosidic linkages, which can be considered in the future as alternative additive in various foods, cosmetic and pharmaceutical preparations.
27474595	6	38	from	foods	1129:1133	arg1	additive					1109:1116	additive	1109:1116	additive	1109:1116	Overall, the results indicate that CNSP have attractive chemical, functional and biological properties, with a preliminary structural may have a backbone of branched 6-O-sulfated (1→4) galactosidic linkages, which can be considered in the future as alternative additive in various foods, cosmetic and pharmaceutical preparations.
27474595	0	39	theme	sulfated	91:98	arg1	polysaccharide					100:113	antihypertensive Cymodocea nodosa sulfated polysaccharide	57:113	antihypertensive Cymodocea nodosa sulfated polysaccharide	57:113	Structural characterization and functional properties of antihypertensive Cymodocea nodosa sulfated polysaccharide.
27474595	2	40	theme	first	210:214	arg1	report					216:221	the first report	206:221	the first report that indicates the chemical composition, structural characterization, functional and antihypertensive properties of this polysaccharide	206:357	This is the first report that indicates the chemical composition, structural characterization, functional and antihypertensive properties of this polysaccharide.
27474595	2	40	theme	first	210:214	arg1	This					198:201	This	198:201	This	198:201	This is the first report that indicates the chemical composition, structural characterization, functional and antihypertensive properties of this polysaccharide.
27474595	6	41	theme	galactosidic	1033:1044	arg1	linkages					1046:1053	branched 6-O-sulfated (1→4) galactosidic linkages	1005:1053	branched 6-O-sulfated (1→4) galactosidic linkages	1005:1053	Overall, the results indicate that CNSP have attractive chemical, functional and biological properties, with a preliminary structural may have a backbone of branched 6-O-sulfated (1→4) galactosidic linkages, which can be considered in the future as alternative additive in various foods, cosmetic and pharmaceutical preparations.
27474595	6	41	theme	galactosidic	1033:1044	arg1	additive					1109:1116	additive	1109:1116	additive	1109:1116	Overall, the results indicate that CNSP have attractive chemical, functional and biological properties, with a preliminary structural may have a backbone of branched 6-O-sulfated (1→4) galactosidic linkages, which can be considered in the future as alternative additive in various foods, cosmetic and pharmaceutical preparations.
27474595	0	42	theme	nodosa	84:89	arg1	polysaccharide					100:113	antihypertensive Cymodocea nodosa sulfated polysaccharide	57:113	antihypertensive Cymodocea nodosa sulfated polysaccharide	57:113	Structural characterization and functional properties of antihypertensive Cymodocea nodosa sulfated polysaccharide.
27474595	1	43	attach	isolated	159:166	arg2	polysaccharide					127:140	A sulfated polysaccharide	116:140	A sulfated polysaccharide	116:140	A sulfated polysaccharide was successfully isolated from Cymodocea nodosa (CNSP).
27474595	1	43	attach	isolated	159:166	arg1	CNSP					191:194	CNSP	191:194	CNSP	191:194	A sulfated polysaccharide was successfully isolated from Cymodocea nodosa (CNSP).
27474595	1	43	attach	isolated	159:166	arg1	nodosa					183:188	Cymodocea nodosa	173:188	Cymodocea nodosa (CNSP)	173:195	A sulfated polysaccharide was successfully isolated from Cymodocea nodosa (CNSP).
27474595	2	44	theme	structural	264:273	arg1	composition					251:261	the chemical composition	238:261	the chemical composition	238:261	This is the first report that indicates the chemical composition, structural characterization, functional and antihypertensive properties of this polysaccharide.
27474595	2	44	theme	structural	264:273	arg1	characterization					275:290	structural characterization	264:290	structural characterization	264:290	This is the first report that indicates the chemical composition, structural characterization, functional and antihypertensive properties of this polysaccharide.
27474595	5	45	theme	dose-dependent	758:771	arg1	manner					773:778	a dose-dependent manner	756:778	a dose-dependent manner using a synthetic substrate, N-hippuryl-His-Leu hydrate salt (HHL)	756:845	It also displayed an important anti-hypertensive activity (IC50=0.43mgml) with a dose-dependent manner using a synthetic substrate, N-hippuryl-His-Leu hydrate salt (HHL).
27474595	6	46	from	cosmetic	1136:1143	arg1	linkages					1046:1053	branched 6-O-sulfated (1→4) galactosidic linkages	1005:1053	branched 6-O-sulfated (1→4) galactosidic linkages	1005:1053	Overall, the results indicate that CNSP have attractive chemical, functional and biological properties, with a preliminary structural may have a backbone of branched 6-O-sulfated (1→4) galactosidic linkages, which can be considered in the future as alternative additive in various foods, cosmetic and pharmaceutical preparations.
27474595	6	46	from	cosmetic	1136:1143	arg1	additive					1109:1116	additive	1109:1116	additive	1109:1116	Overall, the results indicate that CNSP have attractive chemical, functional and biological properties, with a preliminary structural may have a backbone of branched 6-O-sulfated (1→4) galactosidic linkages, which can be considered in the future as alternative additive in various foods, cosmetic and pharmaceutical preparations.
27223251	7	0	theme	chemical	1240:1247	arg1	environment					1264:1274	the chemical and mechanical environment	1236:1274	the chemical and mechanical environment in these gels	1236:1288	Our method provides a simple photochemical tool to postsynthetically control and adjust the chemical and mechanical environment in these gels, as well as the pore microstructure and transport properties.
27223251	5	1	theme	chondrogenic	931:942	arg1	cells					944:948	chondrogenic cells	931:948	chondrogenic cells	931:948	These gels showed promise as scaffolds for cartilage tissue engineering, where the photochemical treatment could be used to tune the properties of the material and ultimately change the growth and extracellular matrix production of chondrogenic cells.
27223251	6	2	theme	ATDC5	951:955	arg1	cells					957:961	ATDC5 cells	951:961	ATDC5 cells cultured on the hydrogels	951:987	ATDC5 cells cultured on the hydrogels showed a greater than 2-fold increase in the production of sulfated glycosaminoglycans (sGAG) in the gels irradiated for 90 min compared to the dark controls.
27223251	2	3	theme	materials	365:373	arg1	composition					303:313	the chemical composition	290:313	the chemical composition	290:313	The photochemistry of Fe(III)-alginate was used to tune the chemical composition, mechanical properties, and microstructure of the materials upon visible light irradiation.
27223251	2	3	theme	materials	365:373	arg1	properties					327:336	mechanical properties	316:336	mechanical properties	316:336	The photochemistry of Fe(III)-alginate was used to tune the chemical composition, mechanical properties, and microstructure of the materials upon visible light irradiation.
27223251	2	3	theme	materials	365:373	arg1	microstructure					343:356	microstructure	343:356	microstructure	343:356	The photochemistry of Fe(III)-alginate was used to tune the chemical composition, mechanical properties, and microstructure of the materials upon visible light irradiation.
27223251	3	4	from	changes	448:454	arg1	properties					472:481	the swelling properties	459:481	the swelling properties	459:481	The photochemical treatment also induced changes in the swelling properties and transport mechanism in the gels due to the changes in material composition and microstructure.
27223251	3	5	from	changes	530:536	arg1	microstructure					566:579	microstructure	566:579	microstructure	566:579	The photochemical treatment also induced changes in the swelling properties and transport mechanism in the gels due to the changes in material composition and microstructure.
27223251	3	5	from	changes	530:536	arg1	composition					550:560	material composition	541:560	material composition	541:560	The photochemical treatment also induced changes in the swelling properties and transport mechanism in the gels due to the changes in material composition and microstructure.
27223251	6	6	theme	2-fold	1011:1016	arg1	increase					1018:1025	a greater than 2-fold increase	996:1025	a greater than 2-fold increase in the production of sulfated glycosaminoglycans (sGAG) in the gels irradiated for 90 min	996:1115	ATDC5 cells cultured on the hydrogels showed a greater than 2-fold increase in the production of sulfated glycosaminoglycans (sGAG) in the gels irradiated for 90 min compared to the dark controls.
27223251	7	7	from	microstructure	1311:1324	arg1	gels					1285:1288	these gels	1279:1288	these gels	1279:1288	Our method provides a simple photochemical tool to postsynthetically control and adjust the chemical and mechanical environment in these gels, as well as the pore microstructure and transport properties.
27223251	2	8	theme	chemical	294:301	arg1	composition					303:313	the chemical composition	290:313	the chemical composition	290:313	The photochemistry of Fe(III)-alginate was used to tune the chemical composition, mechanical properties, and microstructure of the materials upon visible light irradiation.
27223251	6	9	theme	dark	1133:1136	arg1	controls					1138:1145	the dark controls	1129:1145	the dark controls	1129:1145	ATDC5 cells cultured on the hydrogels showed a greater than 2-fold increase in the production of sulfated glycosaminoglycans (sGAG) in the gels irradiated for 90 min compared to the dark controls.
27223251	8	10	theme	performance	1425:1435	arg1	levels					1415:1420	different levels	1405:1420	different levels of performance of these materials	1405:1454	By changing these properties, we could easily access different levels of performance of these materials as substrates for tissue engineering.
27223251	7	11	theme	transport	1330:1338	arg1	properties					1340:1349	transport properties	1330:1349	transport properties	1330:1349	Our method provides a simple photochemical tool to postsynthetically control and adjust the chemical and mechanical environment in these gels, as well as the pore microstructure and transport properties.
27223251	5	12	theme	extracellular	896:908	arg1	matrix					910:915	extracellular matrix	896:915	extracellular matrix production	896:926	These gels showed promise as scaffolds for cartilage tissue engineering, where the photochemical treatment could be used to tune the properties of the material and ultimately change the growth and extracellular matrix production of chondrogenic cells.
27223251	3	13	theme	swelling	463:470	arg1	properties					472:481	the swelling properties	459:481	the swelling properties	459:481	The photochemical treatment also induced changes in the swelling properties and transport mechanism in the gels due to the changes in material composition and microstructure.
27223251	6	14	from	increase	1018:1025	arg1	production					1034:1043	the production	1030:1043	the production of sulfated glycosaminoglycans (sGAG)	1030:1081	ATDC5 cells cultured on the hydrogels showed a greater than 2-fold increase in the production of sulfated glycosaminoglycans (sGAG) in the gels irradiated for 90 min compared to the dark controls.
27223251	6	14	from	increase	1018:1025	arg1	gels					1090:1093	the gels	1086:1093	the gels irradiated for 90 min	1086:1115	ATDC5 cells cultured on the hydrogels showed a greater than 2-fold increase in the production of sulfated glycosaminoglycans (sGAG) in the gels irradiated for 90 min compared to the dark controls.
27223251	3	15	theme	photochemical	411:423	arg1	treatment					425:433	The photochemical treatment	407:433	The photochemical treatment	407:433	The photochemical treatment also induced changes in the swelling properties and transport mechanism in the gels due to the changes in material composition and microstructure.
27223251	2	16	used	used	277:280	arg2	photochemistry					238:251	The photochemistry	234:251	The photochemistry of Fe(III)-alginate	234:271	The photochemistry of Fe(III)-alginate was used to tune the chemical composition, mechanical properties, and microstructure of the materials upon visible light irradiation.
27223251	4	17	theme	mechanical	676:685	arg1	properties					687:696	mechanical properties	676:696	mechanical properties	676:696	The AlgAam gels were biocompatible and could easily be dried and rehydrated with no change in mechanical properties.
27223251	0	18	theme	Polysaccharide-Based	16:35	arg1	Hydrogels					37:45	Photoresponsive Polysaccharide-Based Hydrogels	0:45	Photoresponsive Polysaccharide-Based Hydrogels with Tunable Mechanical Properties for Cartilage Tissue Engineering.	0:114	Photoresponsive Polysaccharide-Based Hydrogels with Tunable Mechanical Properties for Cartilage Tissue Engineering.
27223251	3	19	theme	material	541:548	arg1	composition					550:560	material composition	541:560	material composition	541:560	The photochemical treatment also induced changes in the swelling properties and transport mechanism in the gels due to the changes in material composition and microstructure.
27223251	5	20	theme	photochemical	782:794	arg1	treatment					796:804	the photochemical treatment	778:804	the photochemical treatment	778:804	These gels showed promise as scaffolds for cartilage tissue engineering, where the photochemical treatment could be used to tune the properties of the material and ultimately change the growth and extracellular matrix production of chondrogenic cells.
27223251	1	21	theme	Photoresponsive	116:130	arg1	hydrogels					132:140	Photoresponsive hydrogels	116:140	Photoresponsive hydrogels	116:140	Photoresponsive hydrogels were obtained by coordination of alginate-acrylamide hybrid gels (AlgAam) with ferric ions.
27223251	6	22	theme	irradiated	1095:1104	arg1	gels					1090:1093	the gels	1086:1093	the gels irradiated for 90 min	1086:1115	ATDC5 cells cultured on the hydrogels showed a greater than 2-fold increase in the production of sulfated glycosaminoglycans (sGAG) in the gels irradiated for 90 min compared to the dark controls.
27223251	1	23	theme	ferric	221:226	arg1	ions					228:231	ferric ions	221:231	ferric ions	221:231	Photoresponsive hydrogels were obtained by coordination of alginate-acrylamide hybrid gels (AlgAam) with ferric ions.
27223251	0	24	theme	Photoresponsive	0:14	arg1	Hydrogels					37:45	Photoresponsive Polysaccharide-Based Hydrogels	0:45	Photoresponsive Polysaccharide-Based Hydrogels with Tunable Mechanical Properties for Cartilage Tissue Engineering.	0:114	Photoresponsive Polysaccharide-Based Hydrogels with Tunable Mechanical Properties for Cartilage Tissue Engineering.
27223251	4	25	theme	AlgAam	586:591	arg1	biocompatible					603:615	biocompatible	603:615	biocompatible	603:615	The AlgAam gels were biocompatible and could easily be dried and rehydrated with no change in mechanical properties.
27223251	4	25	theme	AlgAam	586:591	arg1	gels					593:596	The AlgAam gels	582:596	The AlgAam gels	582:596	The AlgAam gels were biocompatible and could easily be dried and rehydrated with no change in mechanical properties.
27223251	1	26	with	coordination	159:170	arg1	ions					228:231	ferric ions	221:231	ferric ions	221:231	Photoresponsive hydrogels were obtained by coordination of alginate-acrylamide hybrid gels (AlgAam) with ferric ions.
27223251	5	27	theme	matrix	910:915	arg1	production					917:926	extracellular matrix production	896:926	extracellular matrix production	896:926	These gels showed promise as scaffolds for cartilage tissue engineering, where the photochemical treatment could be used to tune the properties of the material and ultimately change the growth and extracellular matrix production of chondrogenic cells.
27223251	7	28	theme	photochemical	1177:1189	arg1	tool					1191:1194	a simple photochemical tool	1168:1194	a simple photochemical tool to postsynthetically control and adjust the chemical and mechanical environment in these gels, as well as the pore microstructure and transport properties	1168:1349	Our method provides a simple photochemical tool to postsynthetically control and adjust the chemical and mechanical environment in these gels, as well as the pore microstructure and transport properties.
27223251	2	29	theme	Fe	256:257	arg1	-alginate					263:271	Fe(III)-alginate	256:271	Fe(III)-alginate	256:271	The photochemistry of Fe(III)-alginate was used to tune the chemical composition, mechanical properties, and microstructure of the materials upon visible light irradiation.
27223251	2	30	theme	mechanical	316:325	arg1	properties					327:336	mechanical properties	316:336	mechanical properties	316:336	The photochemistry of Fe(III)-alginate was used to tune the chemical composition, mechanical properties, and microstructure of the materials upon visible light irradiation.
27223251	4	31	from	change	666:671	arg1	properties					687:696	mechanical properties	676:696	mechanical properties	676:696	The AlgAam gels were biocompatible and could easily be dried and rehydrated with no change in mechanical properties.
27223251	5	32	theme	material	850:857	arg1	properties					832:841	the properties	828:841	the properties of the material	828:857	These gels showed promise as scaffolds for cartilage tissue engineering, where the photochemical treatment could be used to tune the properties of the material and ultimately change the growth and extracellular matrix production of chondrogenic cells.
27223251	8	33	theme	tissue	1474:1479	arg1	engineering					1481:1491	tissue engineering	1474:1491	tissue engineering	1474:1491	By changing these properties, we could easily access different levels of performance of these materials as substrates for tissue engineering.
27223251	5	34	theme	cells	944:948	arg1	production					917:926	extracellular matrix production	896:926	extracellular matrix production	896:926	These gels showed promise as scaffolds for cartilage tissue engineering, where the photochemical treatment could be used to tune the properties of the material and ultimately change the growth and extracellular matrix production of chondrogenic cells.
27223251	5	34	theme	cells	944:948	arg1	growth					885:890	growth	885:890	growth	885:890	These gels showed promise as scaffolds for cartilage tissue engineering, where the photochemical treatment could be used to tune the properties of the material and ultimately change the growth and extracellular matrix production of chondrogenic cells.
27223251	7	35	theme	simple	1170:1175	arg1	tool					1191:1194	a simple photochemical tool	1168:1194	a simple photochemical tool to postsynthetically control and adjust the chemical and mechanical environment in these gels, as well as the pore microstructure and transport properties	1168:1349	Our method provides a simple photochemical tool to postsynthetically control and adjust the chemical and mechanical environment in these gels, as well as the pore microstructure and transport properties.
27223251	1	36	theme	hybrid	195:200	arg1	gels					202:205	alginate-acrylamide hybrid gels	175:205	alginate-acrylamide hybrid gels (AlgAam)	175:214	Photoresponsive hydrogels were obtained by coordination of alginate-acrylamide hybrid gels (AlgAam) with ferric ions.
27223251	1	36	theme	hybrid	195:200	arg1	AlgAam					208:213	AlgAam	208:213	AlgAam	208:213	Photoresponsive hydrogels were obtained by coordination of alginate-acrylamide hybrid gels (AlgAam) with ferric ions.
27223251	0	37	theme	Mechanical	60:69	arg1	Properties					71:80	Tunable Mechanical Properties	52:80	Tunable Mechanical Properties	52:80	Photoresponsive Polysaccharide-Based Hydrogels with Tunable Mechanical Properties for Cartilage Tissue Engineering.
27223251	5	38	theme	cartilage	742:750	arg1	engineering					759:769	cartilage tissue engineering	742:769	cartilage tissue engineering	742:769	These gels showed promise as scaffolds for cartilage tissue engineering, where the photochemical treatment could be used to tune the properties of the material and ultimately change the growth and extracellular matrix production of chondrogenic cells.
27223251	7	39	theme	pore	1306:1309	arg1	microstructure					1311:1324	pore microstructure	1306:1324	pore microstructure	1306:1324	Our method provides a simple photochemical tool to postsynthetically control and adjust the chemical and mechanical environment in these gels, as well as the pore microstructure and transport properties.
27223251	0	40	theme	Tunable	52:58	arg1	Properties					71:80	Tunable Mechanical Properties	52:80	Tunable Mechanical Properties	52:80	Photoresponsive Polysaccharide-Based Hydrogels with Tunable Mechanical Properties for Cartilage Tissue Engineering.
27223251	5	41	used	used	815:818	arg2	treatment					796:804	the photochemical treatment	778:804	the photochemical treatment	778:804	These gels showed promise as scaffolds for cartilage tissue engineering, where the photochemical treatment could be used to tune the properties of the material and ultimately change the growth and extracellular matrix production of chondrogenic cells.
27223251	3	42	theme	transport	487:495	arg1	mechanism					497:505	transport mechanism	487:505	transport mechanism	487:505	The photochemical treatment also induced changes in the swelling properties and transport mechanism in the gels due to the changes in material composition and microstructure.
27223251	0	43	with	Hydrogels	37:45	arg1	Properties					71:80	Tunable Mechanical Properties	52:80	Tunable Mechanical Properties	52:80	Photoresponsive Polysaccharide-Based Hydrogels with Tunable Mechanical Properties for Cartilage Tissue Engineering.
27223251	3	44	theme	due	519:521	arg1	gels					514:517	the gels	510:517	the gels due to the changes in material composition and microstructure	510:579	The photochemical treatment also induced changes in the swelling properties and transport mechanism in the gels due to the changes in material composition and microstructure.
27223251	5	45	theme	tissue	752:757	arg1	engineering					759:769	cartilage tissue engineering	742:769	cartilage tissue engineering	742:769	These gels showed promise as scaffolds for cartilage tissue engineering, where the photochemical treatment could be used to tune the properties of the material and ultimately change the growth and extracellular matrix production of chondrogenic cells.
27223251	7	46	theme	mechanical	1253:1262	arg1	environment					1264:1274	the chemical and mechanical environment	1236:1274	the chemical and mechanical environment in these gels	1236:1288	Our method provides a simple photochemical tool to postsynthetically control and adjust the chemical and mechanical environment in these gels, as well as the pore microstructure and transport properties.
27223251	1	47	theme	gels	202:205	arg1	coordination					159:170	coordination	159:170	coordination of alginate-acrylamide hybrid gels (AlgAam) with ferric ions	159:231	Photoresponsive hydrogels were obtained by coordination of alginate-acrylamide hybrid gels (AlgAam) with ferric ions.
27223251	3	48	from	mechanism	497:505	arg1	properties					472:481	the swelling properties	459:481	the swelling properties	459:481	The photochemical treatment also induced changes in the swelling properties and transport mechanism in the gels due to the changes in material composition and microstructure.
27223251	8	49	theme	materials	1446:1454	arg1	performance					1425:1435	performance	1425:1435	performance of these materials	1425:1454	By changing these properties, we could easily access different levels of performance of these materials as substrates for tissue engineering.
27223251	8	50	theme	different	1405:1413	arg1	levels					1415:1420	different levels	1405:1420	different levels of performance of these materials	1405:1454	By changing these properties, we could easily access different levels of performance of these materials as substrates for tissue engineering.
27223251	6	51	dep	2-fold	1011:1016	arg1	greater					998:1004	greater	998:1004	greater	998:1004	ATDC5 cells cultured on the hydrogels showed a greater than 2-fold increase in the production of sulfated glycosaminoglycans (sGAG) in the gels irradiated for 90 min compared to the dark controls.
27223251	6	51	dep	2-fold	1011:1016	arg1	than					1006:1009	than	1006:1009	than	1006:1009	ATDC5 cells cultured on the hydrogels showed a greater than 2-fold increase in the production of sulfated glycosaminoglycans (sGAG) in the gels irradiated for 90 min compared to the dark controls.
27223251	7	52	dep	microstructure	1311:1324	arg1	the					1302:1304	the	1302:1304	the	1302:1304	Our method provides a simple photochemical tool to postsynthetically control and adjust the chemical and mechanical environment in these gels, as well as the pore microstructure and transport properties.
27223251	7	53	from	properties	1340:1349	arg1	gels					1285:1288	these gels	1279:1288	these gels	1279:1288	Our method provides a simple photochemical tool to postsynthetically control and adjust the chemical and mechanical environment in these gels, as well as the pore microstructure and transport properties.
27223251	1	54	theme	alginate-acrylamide	175:193	arg1	gels					202:205	alginate-acrylamide hybrid gels	175:205	alginate-acrylamide hybrid gels (AlgAam)	175:214	Photoresponsive hydrogels were obtained by coordination of alginate-acrylamide hybrid gels (AlgAam) with ferric ions.
27223251	1	54	theme	alginate-acrylamide	175:193	arg1	AlgAam					208:213	AlgAam	208:213	AlgAam	208:213	Photoresponsive hydrogels were obtained by coordination of alginate-acrylamide hybrid gels (AlgAam) with ferric ions.
27223251	6	55	theme	glycosaminoglycans	1057:1074	arg1	production					1034:1043	the production	1030:1043	the production of sulfated glycosaminoglycans (sGAG)	1030:1081	ATDC5 cells cultured on the hydrogels showed a greater than 2-fold increase in the production of sulfated glycosaminoglycans (sGAG) in the gels irradiated for 90 min compared to the dark controls.
27223251	0	56	theme	Tissue	96:101	arg1	Engineering					103:113	Cartilage Tissue Engineering	86:113	Cartilage Tissue Engineering	86:113	Photoresponsive Polysaccharide-Based Hydrogels with Tunable Mechanical Properties for Cartilage Tissue Engineering.
27223251	7	57	from	environment	1264:1274	arg1	gels					1285:1288	these gels	1279:1288	these gels	1279:1288	Our method provides a simple photochemical tool to postsynthetically control and adjust the chemical and mechanical environment in these gels, as well as the pore microstructure and transport properties.
27223251	2	58	theme	light	388:392	arg1	irradiation					394:404	visible light irradiation	380:404	visible light irradiation	380:404	The photochemistry of Fe(III)-alginate was used to tune the chemical composition, mechanical properties, and microstructure of the materials upon visible light irradiation.
27223251	6	59	theme	sulfated	1048:1055	arg1	sGAG					1077:1080	sGAG	1077:1080	sGAG	1077:1080	ATDC5 cells cultured on the hydrogels showed a greater than 2-fold increase in the production of sulfated glycosaminoglycans (sGAG) in the gels irradiated for 90 min compared to the dark controls.
27223251	6	59	theme	sulfated	1048:1055	arg1	glycosaminoglycans					1057:1074	sulfated glycosaminoglycans	1048:1074	sulfated glycosaminoglycans (sGAG)	1048:1081	ATDC5 cells cultured on the hydrogels showed a greater than 2-fold increase in the production of sulfated glycosaminoglycans (sGAG) in the gels irradiated for 90 min compared to the dark controls.
27223251	0	60	theme	Cartilage	86:94	arg1	Engineering					103:113	Cartilage Tissue Engineering	86:113	Cartilage Tissue Engineering	86:113	Photoresponsive Polysaccharide-Based Hydrogels with Tunable Mechanical Properties for Cartilage Tissue Engineering.
27223251	2	61	theme	-alginate	263:271	arg1	photochemistry					238:251	The photochemistry	234:251	The photochemistry of Fe(III)-alginate	234:271	The photochemistry of Fe(III)-alginate was used to tune the chemical composition, mechanical properties, and microstructure of the materials upon visible light irradiation.
27223251	2	62	theme	visible	380:386	arg1	irradiation					394:404	visible light irradiation	380:404	visible light irradiation	380:404	The photochemistry of Fe(III)-alginate was used to tune the chemical composition, mechanical properties, and microstructure of the materials upon visible light irradiation.
28989689	2	0	theme	several	445:451	arg1	diseases					467:474	several prion-related diseases	445:474	several prion-related diseases (transmissible spongiform encephalopathies, TSEs)	445:524	Different prion strains have distinct glycosylation patterns and the extent of glycosylation of potentially pathogenic misfolded prion protein (PrPSc) has a major impact on several prion-related diseases (transmissible spongiform encephalopathies, TSEs).
28989689	2	0	theme	several	445:451	arg1	encephalopathies					502:517	transmissible spongiform encephalopathies	477:517	transmissible spongiform encephalopathies	477:517	Different prion strains have distinct glycosylation patterns and the extent of glycosylation of potentially pathogenic misfolded prion protein (PrPSc) has a major impact on several prion-related diseases (transmissible spongiform encephalopathies, TSEs).
28989689	5	1	theme	secondary	1052:1060	arg1	structure					1062:1070	the secondary structure	1048:1070	the secondary structure	1048:1070	Incorporating PEG at glycosylation sites 181 and 197 in PrP induced only small changes to the secondary structure when compared to unmodified, wildtype PrP.
28989689	9	2	theme	large	1559:1563	arg1	N-glycans					1565:1573	large N-glycans	1559:1573	large N-glycans	1559:1573	This behavior raises the question if large N-glycans interfere with aggregation in vivo and if PEGylated PrP peptides could serve as potential therapeutics.
28989689	5	3	theme	unmodified	1089:1098	arg1	PrP					1110:1112	unmodified, wildtype PrP	1089:1112	unmodified, wildtype PrP	1089:1112	Incorporating PEG at glycosylation sites 181 and 197 in PrP induced only small changes to the secondary structure when compared to unmodified, wildtype PrP.
28989689	5	4	gly	glycosylation	979:991	arg2	sites					993:997	glycosylation sites 181 and 197	979:1009	sites	993:997	Incorporating PEG at glycosylation sites 181 and 197 in PrP induced only small changes to the secondary structure when compared to unmodified, wildtype PrP.
28989689	5	4	gly	glycosylation	979:991	arg2	181					999:1001	181	999:1001	181	999:1001	Incorporating PEG at glycosylation sites 181 and 197 in PrP induced only small changes to the secondary structure when compared to unmodified, wildtype PrP.
28989689	1	5	theme	eukaryotic	197:206	arg1	membranes					213:221	eukaryotic cell membranes	197:221	eukaryotic cell membranes	197:221	The prion protein (PrP) is an N-glycosylated protein attached to the outer leaflet of eukaryotic cell membranes via a glycosylphosphatidylinositol (GPI) anchor.
28989689	5	6	from	sites	993:997	arg1	PrP					1014:1016	PrP	1014:1016	PrP	1014:1016	Incorporating PEG at glycosylation sites 181 and 197 in PrP induced only small changes to the secondary structure when compared to unmodified, wildtype PrP.
28989689	5	6	from	sites	993:997	arg1	PEG					972:974	Incorporating PEG	958:974	Incorporating PEG at glycosylation sites 181 and 197 in PrP	958:1016	Incorporating PEG at glycosylation sites 181 and 197 in PrP induced only small changes to the secondary structure when compared to unmodified, wildtype PrP.
28989689	2	7	contain	have	296:299	arg2	patterns					324:331	distinct glycosylation patterns	301:331	distinct glycosylation patterns	301:331	Different prion strains have distinct glycosylation patterns and the extent of glycosylation of potentially pathogenic misfolded prion protein (PrPSc) has a major impact on several prion-related diseases (transmissible spongiform encephalopathies, TSEs).
28989689	2	7	contain	have	296:299	arg1	strains					288:294	Different prion strains	272:294	Different prion strains	272:294	Different prion strains have distinct glycosylation patterns and the extent of glycosylation of potentially pathogenic misfolded prion protein (PrPSc) has a major impact on several prion-related diseases (transmissible spongiform encephalopathies, TSEs).
28989689	5	8	from	197	1007:1009	arg1	PEG					972:974	Incorporating PEG	958:974	Incorporating PEG at glycosylation sites 181 and 197 in PrP	958:1016	Incorporating PEG at glycosylation sites 181 and 197 in PrP induced only small changes to the secondary structure when compared to unmodified, wildtype PrP.
28989689	2	9	theme	glycosylation	310:322	arg1	patterns					324:331	distinct glycosylation patterns	301:331	distinct glycosylation patterns	301:331	Different prion strains have distinct glycosylation patterns and the extent of glycosylation of potentially pathogenic misfolded prion protein (PrPSc) has a major impact on several prion-related diseases (transmissible spongiform encephalopathies, TSEs).
28989689	3	10	theme	TSEs	805:808	arg1	pathogenesis					789:800	the pathogenesis	785:800	the pathogenesis of TSEs	785:808	Based on these findings it is hypothesized that posttranslational modifications (PTMs) of PrP influence conversion of cellular prion protein (PrPC) into PrPSc and, as such, modified PrP variants are critical tools needed to investigate the impact of PTMs on the pathogenesis of TSEs.
28989689	1	11	theme	cell	208:211	arg1	membranes					213:221	eukaryotic cell membranes	197:221	eukaryotic cell membranes	197:221	The prion protein (PrP) is an N-glycosylated protein attached to the outer leaflet of eukaryotic cell membranes via a glycosylphosphatidylinositol (GPI) anchor.
28989689	0	12	contain	carrying	43:50	arg1	variants					34:41	Semisynthetic prion protein (PrP) variants	0:41	Semisynthetic prion protein (PrP) variants carrying glycan mimics at position 181 and 197	0:88	Semisynthetic prion protein (PrP) variants carrying glycan mimics at position 181 and 197 do not form fibrils.
28989689	0	12	contain	carrying	43:50	arg2	mimics					59:64	glycan mimics	52:64	glycan mimics	52:64	Semisynthetic prion protein (PrP) variants carrying glycan mimics at position 181 and 197 do not form fibrils.
28989689	1	13	theme	membranes	213:221	arg1	leaflet					186:192	the outer leaflet	176:192	the outer leaflet of eukaryotic cell membranes	176:221	The prion protein (PrP) is an N-glycosylated protein attached to the outer leaflet of eukaryotic cell membranes via a glycosylphosphatidylinositol (GPI) anchor.
28989689	2	14	theme	spongiform	491:500	arg1	diseases					467:474	several prion-related diseases	445:474	several prion-related diseases (transmissible spongiform encephalopathies, TSEs)	445:524	Different prion strains have distinct glycosylation patterns and the extent of glycosylation of potentially pathogenic misfolded prion protein (PrPSc) has a major impact on several prion-related diseases (transmissible spongiform encephalopathies, TSEs).
28989689	2	14	theme	spongiform	491:500	arg1	encephalopathies					502:517	transmissible spongiform encephalopathies	477:517	transmissible spongiform encephalopathies	477:517	Different prion strains have distinct glycosylation patterns and the extent of glycosylation of potentially pathogenic misfolded prion protein (PrPSc) has a major impact on several prion-related diseases (transmissible spongiform encephalopathies, TSEs).
28989689	3	15	theme	protein	660:666	arg1	conversion					631:640	conversion	631:640	conversion of cellular prion protein (PrPC) into PrPSc	631:684	Based on these findings it is hypothesized that posttranslational modifications (PTMs) of PrP influence conversion of cellular prion protein (PrPC) into PrPSc and, as such, modified PrP variants are critical tools needed to investigate the impact of PTMs on the pathogenesis of TSEs.
28989689	9	16	theme	PrP	1627:1629	arg1	therapeutics					1665:1676	potential therapeutics	1655:1676	potential therapeutics	1655:1676	This behavior raises the question if large N-glycans interfere with aggregation in vivo and if PEGylated PrP peptides could serve as potential therapeutics.
28989689	9	16	theme	PrP	1627:1629	arg1	peptides					1631:1638	PEGylated PrP peptides	1617:1638	PEGylated PrP peptides	1617:1638	This behavior raises the question if large N-glycans interfere with aggregation in vivo and if PEGylated PrP peptides could serve as potential therapeutics.
28989689	6	17	dep	in	1133:1134	arg1	vitro					1136:1140	vitro	1136:1140	vitro	1136:1140	More importantly, in vitro aggregation was abrogated for all PEGylated PrP variants under conditions at which wildtype PrP aggregated.
28989689	2	18	theme	transmissible	477:489	arg1	diseases					467:474	several prion-related diseases	445:474	several prion-related diseases (transmissible spongiform encephalopathies, TSEs)	445:524	Different prion strains have distinct glycosylation patterns and the extent of glycosylation of potentially pathogenic misfolded prion protein (PrPSc) has a major impact on several prion-related diseases (transmissible spongiform encephalopathies, TSEs).
28989689	2	18	theme	transmissible	477:489	arg1	encephalopathies					502:517	transmissible spongiform encephalopathies	477:517	transmissible spongiform encephalopathies	477:517	Different prion strains have distinct glycosylation patterns and the extent of glycosylation of potentially pathogenic misfolded prion protein (PrPSc) has a major impact on several prion-related diseases (transmissible spongiform encephalopathies, TSEs).
28989689	5	19	theme	small	1031:1035	arg1	changes					1037:1043	only small changes	1026:1043	only small changes to the secondary structure	1026:1070	Incorporating PEG at glycosylation sites 181 and 197 in PrP induced only small changes to the secondary structure when compared to unmodified, wildtype PrP.
28989689	2	20	gly	glycosylation	351:363	arg1	PrPSc					416:420	PrPSc	416:420	PrPSc	416:420	Different prion strains have distinct glycosylation patterns and the extent of glycosylation of potentially pathogenic misfolded prion protein (PrPSc) has a major impact on several prion-related diseases (transmissible spongiform encephalopathies, TSEs).
28989689	2	20	gly	glycosylation	351:363	arg1	protein					407:413	misfolded prion protein	391:413	potentially pathogenic misfolded prion protein (PrPSc)	368:421	Different prion strains have distinct glycosylation patterns and the extent of glycosylation of potentially pathogenic misfolded prion protein (PrPSc) has a major impact on several prion-related diseases (transmissible spongiform encephalopathies, TSEs).
28989689	4	21	theme	monodisperse	890:901	arg1	PEG					923:925	PEG	923:925	PEG	923:925	Here we report a semisynthetic approach to generate PrP variants modified with monodisperse polyethyleneglycol (PEG) units as mimics of N-glycans.
28989689	4	21	theme	monodisperse	890:901	arg1	polyethyleneglycol					903:920	monodisperse polyethyleneglycol	890:920	monodisperse polyethyleneglycol (PEG) units	890:932	Here we report a semisynthetic approach to generate PrP variants modified with monodisperse polyethyleneglycol (PEG) units as mimics of N-glycans.
28989689	2	22	from	impact	435:440	arg1	diseases					467:474	several prion-related diseases	445:474	several prion-related diseases (transmissible spongiform encephalopathies, TSEs)	445:524	Different prion strains have distinct glycosylation patterns and the extent of glycosylation of potentially pathogenic misfolded prion protein (PrPSc) has a major impact on several prion-related diseases (transmissible spongiform encephalopathies, TSEs).
28989689	2	22	from	impact	435:440	arg1	encephalopathies					502:517	transmissible spongiform encephalopathies	477:517	transmissible spongiform encephalopathies	477:517	Different prion strains have distinct glycosylation patterns and the extent of glycosylation of potentially pathogenic misfolded prion protein (PrPSc) has a major impact on several prion-related diseases (transmissible spongiform encephalopathies, TSEs).
28989689	1	23	gly	N-glycosylated	141:154	arg1	protein					156:162	an N-glycosylated protein	138:162	an N-glycosylated protein attached to the outer leaflet of eukaryotic cell membranes via a glycosylphosphatidylinositol (GPI) anchor	138:269	The prion protein (PrP) is an N-glycosylated protein attached to the outer leaflet of eukaryotic cell membranes via a glycosylphosphatidylinositol (GPI) anchor.
28989689	1	23	gly	N-glycosylated	141:154	arg1	protein					121:127	The prion protein	111:127	The prion protein (PrP)	111:133	The prion protein (PrP) is an N-glycosylated protein attached to the outer leaflet of eukaryotic cell membranes via a glycosylphosphatidylinositol (GPI) anchor.
28989689	2	24	contain	has	423:425	arg2	impact					435:440	a major impact	427:440	a major impact on several prion-related diseases (transmissible spongiform encephalopathies, TSEs)	427:524	Different prion strains have distinct glycosylation patterns and the extent of glycosylation of potentially pathogenic misfolded prion protein (PrPSc) has a major impact on several prion-related diseases (transmissible spongiform encephalopathies, TSEs).
28989689	2	24	contain	has	423:425	arg1	extent					341:346	the extent	337:346	the extent of glycosylation of potentially pathogenic misfolded prion protein (PrPSc)	337:421	Different prion strains have distinct glycosylation patterns and the extent of glycosylation of potentially pathogenic misfolded prion protein (PrPSc) has a major impact on several prion-related diseases (transmissible spongiform encephalopathies, TSEs).
28989689	2	25	theme	glycosylation	351:363	arg1	extent					341:346	the extent	337:346	the extent of glycosylation of potentially pathogenic misfolded prion protein (PrPSc)	337:421	Different prion strains have distinct glycosylation patterns and the extent of glycosylation of potentially pathogenic misfolded prion protein (PrPSc) has a major impact on several prion-related diseases (transmissible spongiform encephalopathies, TSEs).
28989689	0	26	theme	prion	14:18	arg1	protein					20:26	Semisynthetic prion protein	0:26	Semisynthetic prion protein (PrP) variants carrying glycan mimics at position 181 and 197	0:88	Semisynthetic prion protein (PrP) variants carrying glycan mimics at position 181 and 197 do not form fibrils.
28989689	0	26	theme	prion	14:18	arg1	PrP					29:31	PrP	29:31	PrP	29:31	Semisynthetic prion protein (PrP) variants carrying glycan mimics at position 181 and 197 do not form fibrils.
28989689	6	27	theme	PEGylated	1176:1184	arg1	variants					1190:1197	all PEGylated PrP variants	1172:1197	all PEGylated PrP variants under conditions at which wildtype PrP aggregated	1172:1247	More importantly, in vitro aggregation was abrogated for all PEGylated PrP variants under conditions at which wildtype PrP aggregated.
28989689	2	28	theme	Different	272:280	arg1	strains					288:294	Different prion strains	272:294	Different prion strains	272:294	Different prion strains have distinct glycosylation patterns and the extent of glycosylation of potentially pathogenic misfolded prion protein (PrPSc) has a major impact on several prion-related diseases (transmissible spongiform encephalopathies, TSEs).
28989689	0	29	theme	Semisynthetic	0:12	arg1	protein					20:26	Semisynthetic prion protein	0:26	Semisynthetic prion protein (PrP) variants carrying glycan mimics at position 181 and 197	0:88	Semisynthetic prion protein (PrP) variants carrying glycan mimics at position 181 and 197 do not form fibrils.
28989689	0	29	theme	Semisynthetic	0:12	arg1	PrP					29:31	PrP	29:31	PrP	29:31	Semisynthetic prion protein (PrP) variants carrying glycan mimics at position 181 and 197 do not form fibrils.
28989689	8	30	theme	synthetic	1393:1401	arg1	segments					1417:1424	synthetic PEGylated PrP segments	1393:1424	synthetic PEGylated PrP segments comprising amino acids 179-231	1393:1455	A similar effect was observed for synthetic PEGylated PrP segments comprising amino acids 179-231 alone if these were added to wildtype PrP in aggregation assays.
28989689	5	31	theme	glycosylation	979:991	arg1	sites					993:997	glycosylation sites 181 and 197	979:1009	sites	993:997	Incorporating PEG at glycosylation sites 181 and 197 in PrP induced only small changes to the secondary structure when compared to unmodified, wildtype PrP.
28989689	1	32	theme	glycosylphosphatidylinositol	229:256	arg1	anchor					264:269	a glycosylphosphatidylinositol (GPI) anchor	227:269	a glycosylphosphatidylinositol (GPI) anchor	227:269	The prion protein (PrP) is an N-glycosylated protein attached to the outer leaflet of eukaryotic cell membranes via a glycosylphosphatidylinositol (GPI) anchor.
28989689	5	33	dep	unmodified	1089:1098	arg1	wildtype					1101:1108	wildtype	1101:1108	wildtype	1101:1108	Incorporating PEG at glycosylation sites 181 and 197 in PrP induced only small changes to the secondary structure when compared to unmodified, wildtype PrP.
28989689	2	34	theme	prion	401:405	arg1	PrPSc					416:420	PrPSc	416:420	PrPSc	416:420	Different prion strains have distinct glycosylation patterns and the extent of glycosylation of potentially pathogenic misfolded prion protein (PrPSc) has a major impact on several prion-related diseases (transmissible spongiform encephalopathies, TSEs).
28989689	2	34	theme	prion	401:405	arg1	protein					407:413	misfolded prion protein	391:413	potentially pathogenic misfolded prion protein (PrPSc)	368:421	Different prion strains have distinct glycosylation patterns and the extent of glycosylation of potentially pathogenic misfolded prion protein (PrPSc) has a major impact on several prion-related diseases (transmissible spongiform encephalopathies, TSEs).
28989689	0	35	theme	protein	20:26	arg1	variants					34:41	Semisynthetic prion protein (PrP) variants	0:41	Semisynthetic prion protein (PrP) variants carrying glycan mimics at position 181 and 197	0:88	Semisynthetic prion protein (PrP) variants carrying glycan mimics at position 181 and 197 do not form fibrils.
28989689	3	36	from	impact	767:772	arg1	pathogenesis					789:800	the pathogenesis	785:800	the pathogenesis of TSEs	785:808	Based on these findings it is hypothesized that posttranslational modifications (PTMs) of PrP influence conversion of cellular prion protein (PrPC) into PrPSc and, as such, modified PrP variants are critical tools needed to investigate the impact of PTMs on the pathogenesis of TSEs.
28989689	3	37	theme	PrP	617:619	arg1	modifications					593:605	posttranslational modifications	575:605	posttranslational modifications (PTMs) of PrP	575:619	Based on these findings it is hypothesized that posttranslational modifications (PTMs) of PrP influence conversion of cellular prion protein (PrPC) into PrPSc and, as such, modified PrP variants are critical tools needed to investigate the impact of PTMs on the pathogenesis of TSEs.
28989689	3	37	theme	PrP	617:619	arg1	PTMs					608:611	PTMs	608:611	PTMs	608:611	Based on these findings it is hypothesized that posttranslational modifications (PTMs) of PrP influence conversion of cellular prion protein (PrPC) into PrPSc and, as such, modified PrP variants are critical tools needed to investigate the impact of PTMs on the pathogenesis of TSEs.
28989689	7	38	theme	wildtype	1314:1321	arg1	PrP					1323:1325	wildtype PrP	1314:1325	wildtype PrP	1314:1325	Furthermore, the addition of PEGylated PrP as low as 10 mol% to wildtype PrP completely blocked aggregation.
28989689	2	39	theme	misfolded	391:399	arg1	PrPSc					416:420	PrPSc	416:420	PrPSc	416:420	Different prion strains have distinct glycosylation patterns and the extent of glycosylation of potentially pathogenic misfolded prion protein (PrPSc) has a major impact on several prion-related diseases (transmissible spongiform encephalopathies, TSEs).
28989689	2	39	theme	misfolded	391:399	arg1	protein					407:413	misfolded prion protein	391:413	potentially pathogenic misfolded prion protein (PrPSc)	368:421	Different prion strains have distinct glycosylation patterns and the extent of glycosylation of potentially pathogenic misfolded prion protein (PrPSc) has a major impact on several prion-related diseases (transmissible spongiform encephalopathies, TSEs).
28989689	1	40	theme	GPI	259:261	arg1	anchor					264:269	a glycosylphosphatidylinositol (GPI) anchor	227:269	a glycosylphosphatidylinositol (GPI) anchor	227:269	The prion protein (PrP) is an N-glycosylated protein attached to the outer leaflet of eukaryotic cell membranes via a glycosylphosphatidylinositol (GPI) anchor.
28989689	2	41	dep	encephalopathies	502:517	arg1	TSEs					520:523	TSEs	520:523	TSEs	520:523	Different prion strains have distinct glycosylation patterns and the extent of glycosylation of potentially pathogenic misfolded prion protein (PrPSc) has a major impact on several prion-related diseases (transmissible spongiform encephalopathies, TSEs).
28989689	8	42	theme	aggregation	1502:1512	arg1	assays					1514:1519	aggregation assays	1502:1519	aggregation assays	1502:1519	A similar effect was observed for synthetic PEGylated PrP segments comprising amino acids 179-231 alone if these were added to wildtype PrP in aggregation assays.
28989689	2	43	theme	pathogenic	380:389	arg1	PrPSc					416:420	PrPSc	416:420	PrPSc	416:420	Different prion strains have distinct glycosylation patterns and the extent of glycosylation of potentially pathogenic misfolded prion protein (PrPSc) has a major impact on several prion-related diseases (transmissible spongiform encephalopathies, TSEs).
28989689	2	43	theme	pathogenic	380:389	arg1	protein					407:413	misfolded prion protein	391:413	potentially pathogenic misfolded prion protein (PrPSc)	368:421	Different prion strains have distinct glycosylation patterns and the extent of glycosylation of potentially pathogenic misfolded prion protein (PrPSc) has a major impact on several prion-related diseases (transmissible spongiform encephalopathies, TSEs).
28989689	2	44	theme	major	429:433	arg1	impact					435:440	a major impact	427:440	a major impact on several prion-related diseases (transmissible spongiform encephalopathies, TSEs)	427:524	Different prion strains have distinct glycosylation patterns and the extent of glycosylation of potentially pathogenic misfolded prion protein (PrPSc) has a major impact on several prion-related diseases (transmissible spongiform encephalopathies, TSEs).
28989689	3	45	theme	PTMs	777:780	arg1	impact					767:772	the impact	763:772	the impact of PTMs on the pathogenesis of TSEs	763:808	Based on these findings it is hypothesized that posttranslational modifications (PTMs) of PrP influence conversion of cellular prion protein (PrPC) into PrPSc and, as such, modified PrP variants are critical tools needed to investigate the impact of PTMs on the pathogenesis of TSEs.
28989689	5	46	theme	Incorporating	958:970	arg1	PEG					972:974	Incorporating PEG	958:974	Incorporating PEG at glycosylation sites 181 and 197 in PrP	958:1016	Incorporating PEG at glycosylation sites 181 and 197 in PrP induced only small changes to the secondary structure when compared to unmodified, wildtype PrP.
28989689	8	47	theme	similar	1361:1367	arg1	effect					1369:1374	A similar effect	1359:1374	A similar effect	1359:1374	A similar effect was observed for synthetic PEGylated PrP segments comprising amino acids 179-231 alone if these were added to wildtype PrP in aggregation assays.
28989689	6	48	theme	wildtype	1225:1232	arg1	PrP					1234:1236	wildtype PrP	1225:1236	wildtype PrP	1225:1236	More importantly, in vitro aggregation was abrogated for all PEGylated PrP variants under conditions at which wildtype PrP aggregated.
28989689	9	49	theme	potential	1655:1663	arg1	therapeutics					1665:1676	potential therapeutics	1655:1676	potential therapeutics	1655:1676	This behavior raises the question if large N-glycans interfere with aggregation in vivo and if PEGylated PrP peptides could serve as potential therapeutics.
28989689	9	49	theme	potential	1655:1663	arg1	peptides					1631:1638	PEGylated PrP peptides	1617:1638	PEGylated PrP peptides	1617:1638	This behavior raises the question if large N-glycans interfere with aggregation in vivo and if PEGylated PrP peptides could serve as potential therapeutics.
28989689	3	50	theme	critical	726:733	arg1	variants					713:720	modified PrP variants	700:720	modified PrP variants	700:720	Based on these findings it is hypothesized that posttranslational modifications (PTMs) of PrP influence conversion of cellular prion protein (PrPC) into PrPSc and, as such, modified PrP variants are critical tools needed to investigate the impact of PTMs on the pathogenesis of TSEs.
28989689	3	50	theme	critical	726:733	arg1	tools					735:739	critical tools	726:739	critical tools needed to investigate the impact of PTMs on the pathogenesis of TSEs	726:808	Based on these findings it is hypothesized that posttranslational modifications (PTMs) of PrP influence conversion of cellular prion protein (PrPC) into PrPSc and, as such, modified PrP variants are critical tools needed to investigate the impact of PTMs on the pathogenesis of TSEs.
28989689	2	51	theme	prion-related	453:465	arg1	diseases					467:474	several prion-related diseases	445:474	several prion-related diseases (transmissible spongiform encephalopathies, TSEs)	445:524	Different prion strains have distinct glycosylation patterns and the extent of glycosylation of potentially pathogenic misfolded prion protein (PrPSc) has a major impact on several prion-related diseases (transmissible spongiform encephalopathies, TSEs).
28989689	2	51	theme	prion-related	453:465	arg1	encephalopathies					502:517	transmissible spongiform encephalopathies	477:517	transmissible spongiform encephalopathies	477:517	Different prion strains have distinct glycosylation patterns and the extent of glycosylation of potentially pathogenic misfolded prion protein (PrPSc) has a major impact on several prion-related diseases (transmissible spongiform encephalopathies, TSEs).
28989689	8	52	theme	PrP	1413:1415	arg1	segments					1417:1424	synthetic PEGylated PrP segments	1393:1424	synthetic PEGylated PrP segments comprising amino acids 179-231	1393:1455	A similar effect was observed for synthetic PEGylated PrP segments comprising amino acids 179-231 alone if these were added to wildtype PrP in aggregation assays.
28989689	2	53	theme	distinct	301:308	arg1	patterns					324:331	distinct glycosylation patterns	301:331	distinct glycosylation patterns	301:331	Different prion strains have distinct glycosylation patterns and the extent of glycosylation of potentially pathogenic misfolded prion protein (PrPSc) has a major impact on several prion-related diseases (transmissible spongiform encephalopathies, TSEs).
28989689	4	54	theme	PrP	863:865	arg1	variants					867:874	PrP variants	863:874	PrP variants modified with monodisperse polyethyleneglycol (PEG) units as mimics of N-glycans	863:955	Here we report a semisynthetic approach to generate PrP variants modified with monodisperse polyethyleneglycol (PEG) units as mimics of N-glycans.
28989689	3	55	theme	PrP	709:711	arg1	variants					713:720	modified PrP variants	700:720	modified PrP variants	700:720	Based on these findings it is hypothesized that posttranslational modifications (PTMs) of PrP influence conversion of cellular prion protein (PrPC) into PrPSc and, as such, modified PrP variants are critical tools needed to investigate the impact of PTMs on the pathogenesis of TSEs.
28989689	3	55	theme	PrP	709:711	arg1	tools					735:739	critical tools	726:739	critical tools needed to investigate the impact of PTMs on the pathogenesis of TSEs	726:808	Based on these findings it is hypothesized that posttranslational modifications (PTMs) of PrP influence conversion of cellular prion protein (PrPC) into PrPSc and, as such, modified PrP variants are critical tools needed to investigate the impact of PTMs on the pathogenesis of TSEs.
28989689	3	56	theme	cellular	645:652	arg1	PrPC					669:672	PrPC	669:672	PrPC	669:672	Based on these findings it is hypothesized that posttranslational modifications (PTMs) of PrP influence conversion of cellular prion protein (PrPC) into PrPSc and, as such, modified PrP variants are critical tools needed to investigate the impact of PTMs on the pathogenesis of TSEs.
28989689	3	56	theme	cellular	645:652	arg1	protein					660:666	cellular prion protein	645:666	cellular prion protein (PrPC)	645:673	Based on these findings it is hypothesized that posttranslational modifications (PTMs) of PrP influence conversion of cellular prion protein (PrPC) into PrPSc and, as such, modified PrP variants are critical tools needed to investigate the impact of PTMs on the pathogenesis of TSEs.
28989689	6	57	theme	PrP	1186:1188	arg1	variants					1190:1197	all PEGylated PrP variants	1172:1197	all PEGylated PrP variants under conditions at which wildtype PrP aggregated	1172:1247	More importantly, in vitro aggregation was abrogated for all PEGylated PrP variants under conditions at which wildtype PrP aggregated.
28989689	3	58	theme	modified	700:707	arg1	variants					713:720	modified PrP variants	700:720	modified PrP variants	700:720	Based on these findings it is hypothesized that posttranslational modifications (PTMs) of PrP influence conversion of cellular prion protein (PrPC) into PrPSc and, as such, modified PrP variants are critical tools needed to investigate the impact of PTMs on the pathogenesis of TSEs.
28989689	3	58	theme	modified	700:707	arg1	tools					735:739	critical tools	726:739	critical tools needed to investigate the impact of PTMs on the pathogenesis of TSEs	726:808	Based on these findings it is hypothesized that posttranslational modifications (PTMs) of PrP influence conversion of cellular prion protein (PrPC) into PrPSc and, as such, modified PrP variants are critical tools needed to investigate the impact of PTMs on the pathogenesis of TSEs.
28989689	4	59	theme	polyethyleneglycol	903:920	arg1	units					928:932	monodisperse polyethyleneglycol (PEG) units	890:932	monodisperse polyethyleneglycol (PEG) units	890:932	Here we report a semisynthetic approach to generate PrP variants modified with monodisperse polyethyleneglycol (PEG) units as mimics of N-glycans.
28989689	7	60	theme	PEGylated	1279:1287	arg1	PrP					1289:1291	PEGylated PrP	1279:1291	PEGylated PrP	1279:1291	Furthermore, the addition of PEGylated PrP as low as 10 mol% to wildtype PrP completely blocked aggregation.
28989689	3	61	theme	prion	654:658	arg1	PrPC					669:672	PrPC	669:672	PrPC	669:672	Based on these findings it is hypothesized that posttranslational modifications (PTMs) of PrP influence conversion of cellular prion protein (PrPC) into PrPSc and, as such, modified PrP variants are critical tools needed to investigate the impact of PTMs on the pathogenesis of TSEs.
28989689	3	61	theme	prion	654:658	arg1	protein					660:666	cellular prion protein	645:666	cellular prion protein (PrPC)	645:673	Based on these findings it is hypothesized that posttranslational modifications (PTMs) of PrP influence conversion of cellular prion protein (PrPC) into PrPSc and, as such, modified PrP variants are critical tools needed to investigate the impact of PTMs on the pathogenesis of TSEs.
28989689	7	62	theme	PrP	1289:1291	arg1	addition					1267:1274	the addition	1263:1274	the addition of PEGylated PrP as low as 10 mol% to wildtype PrP	1263:1325	Furthermore, the addition of PEGylated PrP as low as 10 mol% to wildtype PrP completely blocked aggregation.
28989689	7	63	theme	mol	1306:1308	arg1	%					1309:1309	as low as 10 mol%	1293:1309	as low as 10 mol%	1293:1309	Furthermore, the addition of PEGylated PrP as low as 10 mol% to wildtype PrP completely blocked aggregation.
28989689	4	64	theme	semisynthetic	828:840	arg1	approach					842:849	a semisynthetic approach	826:849	a semisynthetic approach to generate PrP variants modified with monodisperse polyethyleneglycol (PEG) units as mimics of N-glycans	826:955	Here we report a semisynthetic approach to generate PrP variants modified with monodisperse polyethyleneglycol (PEG) units as mimics of N-glycans.
28989689	8	65	theme	PEGylated	1403:1411	arg1	segments					1417:1424	synthetic PEGylated PrP segments	1393:1424	synthetic PEGylated PrP segments comprising amino acids 179-231	1393:1455	A similar effect was observed for synthetic PEGylated PrP segments comprising amino acids 179-231 alone if these were added to wildtype PrP in aggregation assays.
28989689	6	66	theme	in	1133:1134	arg1	aggregation					1142:1152	in vitro aggregation	1133:1152	in vitro aggregation	1133:1152	More importantly, in vitro aggregation was abrogated for all PEGylated PrP variants under conditions at which wildtype PrP aggregated.
28989689	2	67	theme	prion	282:286	arg1	strains					288:294	Different prion strains	272:294	Different prion strains	272:294	Different prion strains have distinct glycosylation patterns and the extent of glycosylation of potentially pathogenic misfolded prion protein (PrPSc) has a major impact on several prion-related diseases (transmissible spongiform encephalopathies, TSEs).
28989689	1	68	theme	outer	180:184	arg1	leaflet					186:192	the outer leaflet	176:192	the outer leaflet of eukaryotic cell membranes	176:221	The prion protein (PrP) is an N-glycosylated protein attached to the outer leaflet of eukaryotic cell membranes via a glycosylphosphatidylinositol (GPI) anchor.
28989689	8	69	theme	amino	1437:1441	arg1	acids					1443:1447	amino acids 179-231	1437:1455	amino acids 179-231	1437:1455	A similar effect was observed for synthetic PEGylated PrP segments comprising amino acids 179-231 alone if these were added to wildtype PrP in aggregation assays.
28989689	1	70	attach	attached	164:171	arg1	leaflet					186:192	the outer leaflet	176:192	the outer leaflet of eukaryotic cell membranes	176:221	The prion protein (PrP) is an N-glycosylated protein attached to the outer leaflet of eukaryotic cell membranes via a glycosylphosphatidylinositol (GPI) anchor.
28989689	1	70	attach	attached	164:171	arg2	protein					156:162	an N-glycosylated protein	138:162	an N-glycosylated protein attached to the outer leaflet of eukaryotic cell membranes via a glycosylphosphatidylinositol (GPI) anchor	138:269	The prion protein (PrP) is an N-glycosylated protein attached to the outer leaflet of eukaryotic cell membranes via a glycosylphosphatidylinositol (GPI) anchor.
28989689	1	70	attach	attached	164:171	arg2	protein					121:127	The prion protein	111:127	The prion protein (PrP)	111:133	The prion protein (PrP) is an N-glycosylated protein attached to the outer leaflet of eukaryotic cell membranes via a glycosylphosphatidylinositol (GPI) anchor.
28989689	3	71	theme	posttranslational	575:591	arg1	modifications					593:605	posttranslational modifications	575:605	posttranslational modifications (PTMs) of PrP	575:619	Based on these findings it is hypothesized that posttranslational modifications (PTMs) of PrP influence conversion of cellular prion protein (PrPC) into PrPSc and, as such, modified PrP variants are critical tools needed to investigate the impact of PTMs on the pathogenesis of TSEs.
28989689	3	71	theme	posttranslational	575:591	arg1	PTMs					608:611	PTMs	608:611	PTMs	608:611	Based on these findings it is hypothesized that posttranslational modifications (PTMs) of PrP influence conversion of cellular prion protein (PrPC) into PrPSc and, as such, modified PrP variants are critical tools needed to investigate the impact of PTMs on the pathogenesis of TSEs.
28989689	1	72	theme	N-glycosylated	141:154	arg1	protein					156:162	an N-glycosylated protein	138:162	an N-glycosylated protein attached to the outer leaflet of eukaryotic cell membranes via a glycosylphosphatidylinositol (GPI) anchor	138:269	The prion protein (PrP) is an N-glycosylated protein attached to the outer leaflet of eukaryotic cell membranes via a glycosylphosphatidylinositol (GPI) anchor.
28989689	1	72	theme	N-glycosylated	141:154	arg1	protein					121:127	The prion protein	111:127	The prion protein (PrP)	111:133	The prion protein (PrP) is an N-glycosylated protein attached to the outer leaflet of eukaryotic cell membranes via a glycosylphosphatidylinositol (GPI) anchor.
28989689	7	73	dep	%	1309:1309	arg1	PrP					1323:1325	wildtype PrP	1314:1325	wildtype PrP	1314:1325	Furthermore, the addition of PEGylated PrP as low as 10 mol% to wildtype PrP completely blocked aggregation.
28989689	0	74	theme	glycan	52:57	arg1	mimics					59:64	glycan mimics	52:64	glycan mimics	52:64	Semisynthetic prion protein (PrP) variants carrying glycan mimics at position 181 and 197 do not form fibrils.
28989689	2	75	theme	protein	407:413	arg1	glycosylation					351:363	glycosylation	351:363	glycosylation of potentially pathogenic misfolded prion protein (PrPSc)	351:421	Different prion strains have distinct glycosylation patterns and the extent of glycosylation of potentially pathogenic misfolded prion protein (PrPSc) has a major impact on several prion-related diseases (transmissible spongiform encephalopathies, TSEs).
28989689	1	76	theme	prion	115:119	arg1	protein					156:162	an N-glycosylated protein	138:162	an N-glycosylated protein attached to the outer leaflet of eukaryotic cell membranes via a glycosylphosphatidylinositol (GPI) anchor	138:269	The prion protein (PrP) is an N-glycosylated protein attached to the outer leaflet of eukaryotic cell membranes via a glycosylphosphatidylinositol (GPI) anchor.
28989689	1	76	theme	prion	115:119	arg1	PrP					130:132	PrP	130:132	PrP	130:132	The prion protein (PrP) is an N-glycosylated protein attached to the outer leaflet of eukaryotic cell membranes via a glycosylphosphatidylinositol (GPI) anchor.
28989689	1	76	theme	prion	115:119	arg1	protein					121:127	The prion protein	111:127	The prion protein (PrP)	111:133	The prion protein (PrP) is an N-glycosylated protein attached to the outer leaflet of eukaryotic cell membranes via a glycosylphosphatidylinositol (GPI) anchor.
28989689	9	77	theme	PEGylated	1617:1625	arg1	therapeutics					1665:1676	potential therapeutics	1655:1676	potential therapeutics	1655:1676	This behavior raises the question if large N-glycans interfere with aggregation in vivo and if PEGylated PrP peptides could serve as potential therapeutics.
28989689	9	77	theme	PEGylated	1617:1625	arg1	peptides					1631:1638	PEGylated PrP peptides	1617:1638	PEGylated PrP peptides	1617:1638	This behavior raises the question if large N-glycans interfere with aggregation in vivo and if PEGylated PrP peptides could serve as potential therapeutics.
28989689	8	78	theme	wildtype	1486:1493	arg1	PrP					1495:1497	wildtype PrP	1486:1497	wildtype PrP	1486:1497	A similar effect was observed for synthetic PEGylated PrP segments comprising amino acids 179-231 alone if these were added to wildtype PrP in aggregation assays.
24299878	6	0	contain	have	750:753	arg2	properties					772:781	gastroprotective properties	755:781	gastroprotective properties which depend on their composition	755:815	PEC κ-CG:chitosan have gastroprotective properties which depend on their composition.
24299878	6	0	contain	have	750:753	arg1	κ-CG					736:739	PEC κ-CG	732:739	PEC κ-CG:chitosan	732:748	PEC κ-CG:chitosan have gastroprotective properties which depend on their composition.
24299878	4	1	theme	supramolecular	424:437	arg1	different					469:477	different	469:477	different	469:477	Using atomic force microscopy we showed that the supramolecular structure of the complexes is different from each other and from the macromolecular structure of the initial polysaccharides.
24299878	4	1	theme	supramolecular	424:437	arg1	structure					439:447	the supramolecular structure	420:447	the supramolecular structure of the complexes	420:464	Using atomic force microscopy we showed that the supramolecular structure of the complexes is different from each other and from the macromolecular structure of the initial polysaccharides.
24299878	7	2	theme	1:10	840:843	arg1	w/w					845:847	chitosan 1:10 w/w	831:847	chitosan 1:10 w/w	831:847	Complex κ-CG:chitosan 1:10 w/w possesses higher gastroprotective activity than the complex 10:1 w/w.
24299878	4	3	theme	macromolecular	508:521	arg1	structure					523:531	the macromolecular structure	504:531	the macromolecular structure of the initial polysaccharides	504:562	Using atomic force microscopy we showed that the supramolecular structure of the complexes is different from each other and from the macromolecular structure of the initial polysaccharides.
24299878	3	4	theme	complex	271:277	arg1	formation					279:287	The complex formation	267:287	The complex formation of κ-CG	267:295	The complex formation of κ-CG:chitosan 1:10 and 10:1 w/w was shown by centrifugation in a Percoll gradient.
24299878	3	5	from	centrifugation	337:350	arg1	gradient					365:372	a Percoll gradient	355:372	a Percoll gradient	355:372	The complex formation of κ-CG:chitosan 1:10 and 10:1 w/w was shown by centrifugation in a Percoll gradient.
24299878	6	6	theme	gastroprotective	755:770	arg1	properties					772:781	gastroprotective properties	755:781	gastroprotective properties which depend on their composition	755:815	PEC κ-CG:chitosan have gastroprotective properties which depend on their composition.
24299878	8	7	theme	direct	1100:1105	arg1	contact					1107:1113	a direct contact	1098:1113	a direct contact with the ulcerogenic agent	1098:1140	These results suggest that the gastroprotective effect of complexes can be associated with their protective layer on the surface of the mucous membrane of a stomach, which avoids a direct contact with the ulcerogenic agent.
24299878	8	8	theme	gastroprotective	950:965	arg1	effect					967:972	the gastroprotective effect	946:972	the gastroprotective effect of complexes	946:985	These results suggest that the gastroprotective effect of complexes can be associated with their protective layer on the surface of the mucous membrane of a stomach, which avoids a direct contact with the ulcerogenic agent.
24299878	5	9	theme	κ-CG	617:620	arg1	effect					607:612	The gastroprotective and anti-ulcerogenic effect	565:612	The gastroprotective and anti-ulcerogenic effect of κ-CG, chitosan and their complexes	565:650	The gastroprotective and anti-ulcerogenic effect of κ-CG, chitosan and their complexes was investigated on the model of stomach ulcers induced by indometacin in rats.
24299878	2	10	dep	value	197:201	arg1	-1					220:221	-1	220:221	-1	220:221	Binding constant value (2.11 × 10(7)mol(-1)) showed high affinity of κ-CG to chitosan.
24299878	2	10	dep	value	197:201	arg1	mol					216:218	2.11 × 10(7)mol	204:218	2.11 × 10(7)mol(-1)	204:222	Binding constant value (2.11 × 10(7)mol(-1)) showed high affinity of κ-CG to chitosan.
24299878	4	11	from	structure	523:531	arg1	different					469:477	different	469:477	different	469:477	Using atomic force microscopy we showed that the supramolecular structure of the complexes is different from each other and from the macromolecular structure of the initial polysaccharides.
24299878	8	12	theme	membrane	1062:1069	arg1	surface					1040:1046	the surface	1036:1046	the surface of the mucous membrane of a stomach, which avoids a direct contact with the ulcerogenic agent	1036:1140	These results suggest that the gastroprotective effect of complexes can be associated with their protective layer on the surface of the mucous membrane of a stomach, which avoids a direct contact with the ulcerogenic agent.
24299878	3	13	theme	κ-CG	292:295	arg1	formation					279:287	The complex formation	267:287	The complex formation of κ-CG	267:295	The complex formation of κ-CG:chitosan 1:10 and 10:1 w/w was shown by centrifugation in a Percoll gradient.
24299878	3	13	theme	κ-CG	292:295	arg1	w/w					320:322	10:1 w/w	315:322	10:1 w/w	315:322	The complex formation of κ-CG:chitosan 1:10 and 10:1 w/w was shown by centrifugation in a Percoll gradient.
24299878	3	13	theme	κ-CG	292:295	arg1	chitosan					297:304	chitosan 1:10	297:309	chitosan 1:10	297:309	The complex formation of κ-CG:chitosan 1:10 and 10:1 w/w was shown by centrifugation in a Percoll gradient.
24299878	7	14	contain	possesses	849:857	arg2	activity					883:890	higher gastroprotective activity	859:890	higher gastroprotective activity than the complex 10:1 w/w	859:916	Complex κ-CG:chitosan 1:10 w/w possesses higher gastroprotective activity than the complex 10:1 w/w.
24299878	7	14	contain	possesses	849:857	arg1	w/w					845:847	chitosan 1:10 w/w	831:847	chitosan 1:10 w/w	831:847	Complex κ-CG:chitosan 1:10 w/w possesses higher gastroprotective activity than the complex 10:1 w/w.
24299878	1	15	theme	soluble	96:102	arg1	PEC					131:133	PEC	131:133	PEC	131:133	The soluble polyelectrolyte complexes (PEC) κ-carrageenan (κ-CG):chitosan was obtained.
24299878	1	15	theme	soluble	96:102	arg1	complexes					120:128	soluble polyelectrolyte complexes	96:128	The soluble polyelectrolyte complexes (PEC) κ-carrageenan (κ-CG):chitosan was obtained.	92:178	The soluble polyelectrolyte complexes (PEC) κ-carrageenan (κ-CG):chitosan was obtained.
24299878	7	16	theme	Complex	818:824	arg1	κ-CG					826:829	Complex κ-CG	818:829	Complex κ-CG:chitosan 1:10 w/w possesses higher gastroprotective activity than the complex 10:1 w/w.	818:917	Complex κ-CG:chitosan 1:10 w/w possesses higher gastroprotective activity than the complex 10:1 w/w.
24299878	5	17	theme	ulcers	693:698	arg1	model					676:680	the model	672:680	the model of stomach ulcers induced by indometacin in rats	672:729	The gastroprotective and anti-ulcerogenic effect of κ-CG, chitosan and their complexes was investigated on the model of stomach ulcers induced by indometacin in rats.
24299878	5	18	theme	gastroprotective	569:584	arg1	effect					607:612	The gastroprotective and anti-ulcerogenic effect	565:612	The gastroprotective and anti-ulcerogenic effect of κ-CG, chitosan and their complexes	565:650	The gastroprotective and anti-ulcerogenic effect of κ-CG, chitosan and their complexes was investigated on the model of stomach ulcers induced by indometacin in rats.
24299878	7	19	dep	κ-CG	826:829	arg1	possesses					849:857	possesses	849:857	possesses higher gastroprotective activity than the complex 10:1 w/w	849:916	Complex κ-CG:chitosan 1:10 w/w possesses higher gastroprotective activity than the complex 10:1 w/w.
24299878	1	20	theme	polyelectrolyte	104:118	arg1	PEC					131:133	PEC	131:133	PEC	131:133	The soluble polyelectrolyte complexes (PEC) κ-carrageenan (κ-CG):chitosan was obtained.
24299878	1	20	theme	polyelectrolyte	104:118	arg1	complexes					120:128	soluble polyelectrolyte complexes	96:128	The soluble polyelectrolyte complexes (PEC) κ-carrageenan (κ-CG):chitosan was obtained.	92:178	The soluble polyelectrolyte complexes (PEC) κ-carrageenan (κ-CG):chitosan was obtained.
24299878	6	21	dep	κ-CG	736:739	arg1	chitosan					741:748	chitosan	741:748	PEC κ-CG:chitosan	732:748	PEC κ-CG:chitosan have gastroprotective properties which depend on their composition.
24299878	5	22	theme	stomach	685:691	arg1	ulcers					693:698	stomach ulcers	685:698	stomach ulcers induced by indometacin in rats	685:729	The gastroprotective and anti-ulcerogenic effect of κ-CG, chitosan and their complexes was investigated on the model of stomach ulcers induced by indometacin in rats.
24299878	8	23	theme	ulcerogenic	1124:1134	arg1	agent					1136:1140	the ulcerogenic agent	1120:1140	the ulcerogenic agent	1120:1140	These results suggest that the gastroprotective effect of complexes can be associated with their protective layer on the surface of the mucous membrane of a stomach, which avoids a direct contact with the ulcerogenic agent.
24299878	6	24	theme	PEC	732:734	arg1	κ-CG					736:739	PEC κ-CG	732:739	PEC κ-CG:chitosan	732:748	PEC κ-CG:chitosan have gastroprotective properties which depend on their composition.
24299878	1	25	theme	complexes	120:128	arg1	κ-CG					151:154	κ-CG	151:154	κ-CG	151:154	The soluble polyelectrolyte complexes (PEC) κ-carrageenan (κ-CG):chitosan was obtained.
24299878	1	25	theme	complexes	120:128	arg1	κ-carrageenan					136:148	The soluble polyelectrolyte complexes (PEC) κ-carrageenan	92:148	The soluble polyelectrolyte complexes (PEC) κ-carrageenan (κ-CG):chitosan was obtained.	92:178	The soluble polyelectrolyte complexes (PEC) κ-carrageenan (κ-CG):chitosan was obtained.
24299878	0	26	theme	Soluble	0:6	arg1	chitosan-carrageenan					8:27	Soluble chitosan-carrageenan	0:27	Soluble chitosan-carrageenan	0:27	Soluble chitosan-carrageenan polyelectrolyte complexes and their gastroprotective activity.
24299878	5	27	theme	anti-ulcerogenic	590:605	arg1	effect					607:612	The gastroprotective and anti-ulcerogenic effect	565:612	The gastroprotective and anti-ulcerogenic effect of κ-CG, chitosan and their complexes	565:650	The gastroprotective and anti-ulcerogenic effect of κ-CG, chitosan and their complexes was investigated on the model of stomach ulcers induced by indometacin in rats.
24299878	8	28	theme	complexes	977:985	arg1	effect					967:972	the gastroprotective effect	946:972	the gastroprotective effect of complexes	946:985	These results suggest that the gastroprotective effect of complexes can be associated with their protective layer on the surface of the mucous membrane of a stomach, which avoids a direct contact with the ulcerogenic agent.
24299878	8	29	theme	protective	1016:1025	arg1	layer					1027:1031	their protective layer	1010:1031	their protective layer on the surface of the mucous membrane of a stomach, which avoids a direct contact with the ulcerogenic agent	1010:1140	These results suggest that the gastroprotective effect of complexes can be associated with their protective layer on the surface of the mucous membrane of a stomach, which avoids a direct contact with the ulcerogenic agent.
24299878	3	30	theme	Percoll	357:363	arg1	gradient					365:372	a Percoll gradient	355:372	a Percoll gradient	355:372	The complex formation of κ-CG:chitosan 1:10 and 10:1 w/w was shown by centrifugation in a Percoll gradient.
24299878	4	31	from	other	489:493	arg1	different					469:477	different	469:477	different	469:477	Using atomic force microscopy we showed that the supramolecular structure of the complexes is different from each other and from the macromolecular structure of the initial polysaccharides.
24299878	4	31	from	other	489:493	arg1	structure					439:447	the supramolecular structure	420:447	the supramolecular structure of the complexes	420:464	Using atomic force microscopy we showed that the supramolecular structure of the complexes is different from each other and from the macromolecular structure of the initial polysaccharides.
24299878	3	32	theme	10:1	315:318	arg1	w/w					320:322	10:1 w/w	315:322	10:1 w/w	315:322	The complex formation of κ-CG:chitosan 1:10 and 10:1 w/w was shown by centrifugation in a Percoll gradient.
24299878	4	33	dep	showed	408:413	arg1	Using					375:379	Using	375:379	Using atomic force microscopy	375:403	Using atomic force microscopy we showed that the supramolecular structure of the complexes is different from each other and from the macromolecular structure of the initial polysaccharides.
24299878	1	34	dep	κ-carrageenan	136:148	arg1	obtained					170:177	obtained	170:177	was obtained	166:177	The soluble polyelectrolyte complexes (PEC) κ-carrageenan (κ-CG):chitosan was obtained.
24299878	4	35	theme	atomic	381:386	arg1	microscopy					394:403	atomic force microscopy	381:403	atomic force microscopy	381:403	Using atomic force microscopy we showed that the supramolecular structure of the complexes is different from each other and from the macromolecular structure of the initial polysaccharides.
24299878	2	36	theme	κ-CG	249:252	arg1	affinity					237:244	high affinity	232:244	high affinity of κ-CG to chitosan	232:264	Binding constant value (2.11 × 10(7)mol(-1)) showed high affinity of κ-CG to chitosan.
24299878	2	37	theme	constant	188:195	arg1	value					197:201	Binding constant value	180:201	Binding constant value (2.11 × 10(7)mol(-1))	180:223	Binding constant value (2.11 × 10(7)mol(-1)) showed high affinity of κ-CG to chitosan.
24299878	2	38	theme	Binding	180:186	arg1	value					197:201	Binding constant value	180:201	Binding constant value (2.11 × 10(7)mol(-1))	180:223	Binding constant value (2.11 × 10(7)mol(-1)) showed high affinity of κ-CG to chitosan.
24299878	4	39	theme	complexes	456:464	arg1	different					469:477	different	469:477	different	469:477	Using atomic force microscopy we showed that the supramolecular structure of the complexes is different from each other and from the macromolecular structure of the initial polysaccharides.
24299878	4	39	theme	complexes	456:464	arg1	structure					439:447	the supramolecular structure	420:447	the supramolecular structure of the complexes	420:464	Using atomic force microscopy we showed that the supramolecular structure of the complexes is different from each other and from the macromolecular structure of the initial polysaccharides.
24299878	5	40	theme	chitosan	623:630	arg1	effect					607:612	The gastroprotective and anti-ulcerogenic effect	565:612	The gastroprotective and anti-ulcerogenic effect of κ-CG, chitosan and their complexes	565:650	The gastroprotective and anti-ulcerogenic effect of κ-CG, chitosan and their complexes was investigated on the model of stomach ulcers induced by indometacin in rats.
24299878	4	41	theme	force	388:392	arg1	microscopy					394:403	atomic force microscopy	381:403	atomic force microscopy	381:403	Using atomic force microscopy we showed that the supramolecular structure of the complexes is different from each other and from the macromolecular structure of the initial polysaccharides.
24299878	0	42	theme	gastroprotective	65:80	arg1	activity					82:89	their gastroprotective activity	59:89	their gastroprotective activity	59:89	Soluble chitosan-carrageenan polyelectrolyte complexes and their gastroprotective activity.
24299878	8	43	theme	mucous	1055:1060	arg1	membrane					1062:1069	the mucous membrane	1051:1069	the mucous membrane of a stomach, which avoids a direct contact with the ulcerogenic agent	1051:1140	These results suggest that the gastroprotective effect of complexes can be associated with their protective layer on the surface of the mucous membrane of a stomach, which avoids a direct contact with the ulcerogenic agent.
24299878	2	44	theme	high	232:235	arg1	affinity					237:244	high affinity	232:244	high affinity of κ-CG to chitosan	232:264	Binding constant value (2.11 × 10(7)mol(-1)) showed high affinity of κ-CG to chitosan.
24299878	7	45	theme	higher	859:864	arg1	activity					883:890	higher gastroprotective activity	859:890	higher gastroprotective activity than the complex 10:1 w/w	859:916	Complex κ-CG:chitosan 1:10 w/w possesses higher gastroprotective activity than the complex 10:1 w/w.
24299878	2	46	theme	×	209:209	arg1	-1					220:221	-1	220:221	-1	220:221	Binding constant value (2.11 × 10(7)mol(-1)) showed high affinity of κ-CG to chitosan.
24299878	2	46	theme	×	209:209	arg1	mol					216:218	2.11 × 10(7)mol	204:218	2.11 × 10(7)mol(-1)	204:222	Binding constant value (2.11 × 10(7)mol(-1)) showed high affinity of κ-CG to chitosan.
24299878	7	47	theme	gastroprotective	866:881	arg1	activity					883:890	higher gastroprotective activity	859:890	higher gastroprotective activity than the complex 10:1 w/w	859:916	Complex κ-CG:chitosan 1:10 w/w possesses higher gastroprotective activity than the complex 10:1 w/w.
24299878	4	48	theme	initial	540:546	arg1	polysaccharides					548:562	the initial polysaccharides	536:562	the initial polysaccharides	536:562	Using atomic force microscopy we showed that the supramolecular structure of the complexes is different from each other and from the macromolecular structure of the initial polysaccharides.
24299878	5	49	theme	complexes	642:650	arg1	effect					607:612	The gastroprotective and anti-ulcerogenic effect	565:612	The gastroprotective and anti-ulcerogenic effect of κ-CG, chitosan and their complexes	565:650	The gastroprotective and anti-ulcerogenic effect of κ-CG, chitosan and their complexes was investigated on the model of stomach ulcers induced by indometacin in rats.
24299878	8	50	from	layer	1027:1031	arg1	surface					1040:1046	the surface	1036:1046	the surface of the mucous membrane of a stomach, which avoids a direct contact with the ulcerogenic agent	1036:1140	These results suggest that the gastroprotective effect of complexes can be associated with their protective layer on the surface of the mucous membrane of a stomach, which avoids a direct contact with the ulcerogenic agent.
24299878	8	51	with	contact	1107:1113	arg1	agent					1136:1140	the ulcerogenic agent	1120:1140	the ulcerogenic agent	1120:1140	These results suggest that the gastroprotective effect of complexes can be associated with their protective layer on the surface of the mucous membrane of a stomach, which avoids a direct contact with the ulcerogenic agent.
24299878	8	52	theme	stomach	1076:1082	arg1	membrane					1062:1069	the mucous membrane	1051:1069	the mucous membrane of a stomach, which avoids a direct contact with the ulcerogenic agent	1051:1140	These results suggest that the gastroprotective effect of complexes can be associated with their protective layer on the surface of the mucous membrane of a stomach, which avoids a direct contact with the ulcerogenic agent.
24299878	7	53	theme	chitosan	831:838	arg1	w/w					845:847	chitosan 1:10 w/w	831:847	chitosan 1:10 w/w	831:847	Complex κ-CG:chitosan 1:10 w/w possesses higher gastroprotective activity than the complex 10:1 w/w.
24299878	4	54	theme	polysaccharides	548:562	arg1	structure					523:531	the macromolecular structure	504:531	the macromolecular structure of the initial polysaccharides	504:562	Using atomic force microscopy we showed that the supramolecular structure of the complexes is different from each other and from the macromolecular structure of the initial polysaccharides.
25655826	10	0	theme	root	1410:1413	arg1	elongation					1420:1429	root hair elongation	1410:1429	root hair elongation	1410:1429	Taken together, our results support a model in which correct peptidyl-proline hydroxylation on EXTs, and possibly in other HRGPs, is required for proper cell wall self-assembly and hence root hair elongation in Arabidopsis thaliana.
25655826	4	1	theme	Proline	458:464	arg1	modification					509:520	an early post-translational modification	481:520	an early post-translational modification (PTM) of HRGPs catalyzed by prolyl 4-hydroxylases (P4Hs)	481:577	Proline hydroxylation, an early post-translational modification (PTM) of HRGPs catalyzed by prolyl 4-hydroxylases (P4Hs), defines their subsequent O-glycosylation sites.
25655826	4	1	theme	Proline	458:464	arg1	hydroxylation					466:478	Proline hydroxylation	458:478	Proline hydroxylation	458:478	Proline hydroxylation, an early post-translational modification (PTM) of HRGPs catalyzed by prolyl 4-hydroxylases (P4Hs), defines their subsequent O-glycosylation sites.
25655826	9	2	from	impact	1150:1155	arg1	architecture					1209:1220	the cell wall architecture	1195:1220	the cell wall architecture	1195:1220	Finally, we explore the impact of deficient proline hydroxylation on the cell wall architecture.
25655826	10	3	theme	cell	1376:1379	arg1	self-assembly					1386:1398	proper cell wall self-assembly	1369:1398	proper cell wall self-assembly	1369:1398	Taken together, our results support a model in which correct peptidyl-proline hydroxylation on EXTs, and possibly in other HRGPs, is required for proper cell wall self-assembly and hence root hair elongation in Arabidopsis thaliana.
25655826	9	4	theme	deficient	1160:1168	arg1	hydroxylation					1178:1190	deficient proline hydroxylation	1160:1190	deficient proline hydroxylation	1160:1190	Finally, we explore the impact of deficient proline hydroxylation on the cell wall architecture.
25655826	6	5	theme	EXT	829:831	arg1	substrates					833:842	EXT substrates	829:842	EXT substrates	829:842	Second, we demonstrate that P4H5 has in vitro preferred specificity for EXT substrates rather than for other HRGPs.
25655826	10	6	from	hydroxylation	1301:1313	arg1	EXTs					1318:1321	EXTs	1318:1321	EXTs	1318:1321	Taken together, our results support a model in which correct peptidyl-proline hydroxylation on EXTs, and possibly in other HRGPs, is required for proper cell wall self-assembly and hence root hair elongation in Arabidopsis thaliana.
25655826	10	6	from	hydroxylation	1301:1313	arg1	HRGPs					1346:1350	other HRGPs	1340:1350	other HRGPs	1340:1350	Taken together, our results support a model in which correct peptidyl-proline hydroxylation on EXTs, and possibly in other HRGPs, is required for proper cell wall self-assembly and hence root hair elongation in Arabidopsis thaliana.
25655826	9	7	theme	hydroxylation	1178:1190	arg1	impact					1150:1155	the impact	1146:1155	the impact of deficient proline hydroxylation on the cell wall architecture	1146:1220	Finally, we explore the impact of deficient proline hydroxylation on the cell wall architecture.
25655826	2	8	theme	tip	261:263	arg1	growth					265:270	tip growth	261:270	tip growth	261:270	However, structural plant cell walls impose constraints to accomplish tip growth.
25655826	10	9	from	self-assembly	1386:1398	arg1	thaliana					1446:1453	Arabidopsis thaliana	1434:1453	Arabidopsis thaliana	1434:1453	Taken together, our results support a model in which correct peptidyl-proline hydroxylation on EXTs, and possibly in other HRGPs, is required for proper cell wall self-assembly and hence root hair elongation in Arabidopsis thaliana.
25655826	1	10	theme	Root	73:76	arg1	hairs					78:82	Root hairs	73:82	Root hairs	73:82	Root hairs are single cells that develop by tip growth, a process shared with pollen tubes, axons, and fungal hyphae.
25655826	1	10	theme	Root	73:76	arg1	cells					95:99	single cells	88:99	single cells that develop by tip growth, a process shared with pollen tubes, axons, and fungal hyphae	88:188	Root hairs are single cells that develop by tip growth, a process shared with pollen tubes, axons, and fungal hyphae.
25655826	4	11	theme	prolyl	550:555	arg1	P4Hs					573:576	P4Hs	573:576	P4Hs	573:576	Proline hydroxylation, an early post-translational modification (PTM) of HRGPs catalyzed by prolyl 4-hydroxylases (P4Hs), defines their subsequent O-glycosylation sites.
25655826	4	11	theme	prolyl	550:555	arg1	4-hydroxylases					557:570	prolyl 4-hydroxylases	550:570	prolyl 4-hydroxylases (P4Hs)	550:577	Proline hydroxylation, an early post-translational modification (PTM) of HRGPs catalyzed by prolyl 4-hydroxylases (P4Hs), defines their subsequent O-glycosylation sites.
25655826	3	12	theme	cell	311:314	arg1	walls					316:320	plant cell walls	305:320	plant cell walls	305:320	In addition to polysaccharides, plant cell walls are composed of hydroxyproline-rich glycoproteins (HRGPs), which include several groups of O-glycoproteins, including extensins (EXTs).
25655826	6	13	theme	preferred	803:811	arg1	specificity					813:823	in vitro preferred specificity	794:823	in vitro preferred specificity for EXT substrates rather than for other HRGPs	794:870	Second, we demonstrate that P4H5 has in vitro preferred specificity for EXT substrates rather than for other HRGPs.
25655826	1	14	theme	pollen	151:156	arg1	tubes					158:162	pollen tubes	151:162	pollen tubes	151:162	Root hairs are single cells that develop by tip growth, a process shared with pollen tubes, axons, and fungal hyphae.
25655826	8	15	theme	secretory	1062:1070	arg1	pathway					1072:1078	the secretory pathway	1058:1078	the secretory pathway	1058:1078	These three P4Hs are shown to be targeted to the secretory pathway, where P4H5 forms dimers with P4H2 and P4H13.
25655826	6	16	theme	in vitro	794:801	arg1	specificity					813:823	in vitro preferred specificity	794:823	in vitro preferred specificity for EXT substrates rather than for other HRGPs	794:870	Second, we demonstrate that P4H5 has in vitro preferred specificity for EXT substrates rather than for other HRGPs.
25655826	3	17	gly	glycoproteins	358:370	arg1	glycoproteins					358:370	hydroxyproline-rich glycoproteins	338:370	hydroxyproline-rich glycoproteins (HRGPs)	338:378	In addition to polysaccharides, plant cell walls are composed of hydroxyproline-rich glycoproteins (HRGPs), which include several groups of O-glycoproteins, including extensins (EXTs).
25655826	3	17	gly	glycoproteins	358:370	arg1	HRGPs					373:377	HRGPs	373:377	HRGPs	373:377	In addition to polysaccharides, plant cell walls are composed of hydroxyproline-rich glycoproteins (HRGPs), which include several groups of O-glycoproteins, including extensins (EXTs).
25655826	5	18	theme	root	735:738	arg1	growth					749:754	root hair tip growth	735:754	root hair tip growth	735:754	In this work, our genetic analyses prove that P4H5, and to a lesser extent P4H2 and P4H13, are pivotal for root hair tip growth.
25655826	10	19	theme	Arabidopsis	1434:1444	arg1	thaliana					1446:1453	Arabidopsis thaliana	1434:1453	Arabidopsis thaliana	1434:1453	Taken together, our results support a model in which correct peptidyl-proline hydroxylation on EXTs, and possibly in other HRGPs, is required for proper cell wall self-assembly and hence root hair elongation in Arabidopsis thaliana.
25655826	9	20	theme	wall	1204:1207	arg1	architecture					1209:1220	the cell wall architecture	1195:1220	the cell wall architecture	1195:1220	Finally, we explore the impact of deficient proline hydroxylation on the cell wall architecture.
25655826	10	21	theme	other	1340:1344	arg1	HRGPs					1346:1350	other HRGPs	1340:1350	other HRGPs	1340:1350	Taken together, our results support a model in which correct peptidyl-proline hydroxylation on EXTs, and possibly in other HRGPs, is required for proper cell wall self-assembly and hence root hair elongation in Arabidopsis thaliana.
25655826	1	22	theme	single	88:93	arg1	hairs					78:82	Root hairs	73:82	Root hairs	73:82	Root hairs are single cells that develop by tip growth, a process shared with pollen tubes, axons, and fungal hyphae.
25655826	1	22	theme	single	88:93	arg1	cells					95:99	single cells	88:99	single cells that develop by tip growth, a process shared with pollen tubes, axons, and fungal hyphae	88:188	Root hairs are single cells that develop by tip growth, a process shared with pollen tubes, axons, and fungal hyphae.
25655826	4	23	theme	O-glycosylation	605:619	arg1	sites					621:625	their subsequent O-glycosylation sites	588:625	their subsequent O-glycosylation sites	588:625	Proline hydroxylation, an early post-translational modification (PTM) of HRGPs catalyzed by prolyl 4-hydroxylases (P4Hs), defines their subsequent O-glycosylation sites.
25655826	0	24	theme	Complex	0:6	arg1	regulation					8:17	Complex regulation	0:17	Complex regulation of prolyl-4-hydroxylases	0:42	Complex regulation of prolyl-4-hydroxylases impacts root hair expansion.
25655826	4	25	gly	O-glycosylation	605:619	arg2	sites					621:625	their subsequent O-glycosylation sites	588:625	their subsequent O-glycosylation sites	588:625	Proline hydroxylation, an early post-translational modification (PTM) of HRGPs catalyzed by prolyl 4-hydroxylases (P4Hs), defines their subsequent O-glycosylation sites.
25655826	10	26	theme	peptidyl-proline	1284:1299	arg1	hydroxylation					1301:1313	correct peptidyl-proline hydroxylation	1276:1313	correct peptidyl-proline hydroxylation	1276:1313	Taken together, our results support a model in which correct peptidyl-proline hydroxylation on EXTs, and possibly in other HRGPs, is required for proper cell wall self-assembly and hence root hair elongation in Arabidopsis thaliana.
25655826	3	27	theme	several	395:401	arg1	groups					403:408	several groups	395:408	several groups	395:408	In addition to polysaccharides, plant cell walls are composed of hydroxyproline-rich glycoproteins (HRGPs), which include several groups of O-glycoproteins, including extensins (EXTs).
25655826	3	27	theme	several	395:401	arg1	O-glycoproteins					413:427	O-glycoproteins	413:427	O-glycoproteins	413:427	In addition to polysaccharides, plant cell walls are composed of hydroxyproline-rich glycoproteins (HRGPs), which include several groups of O-glycoproteins, including extensins (EXTs).
25655826	3	27	theme	several	395:401	arg1	extensins					440:448	extensins	440:448	extensins (EXTs)	440:455	In addition to polysaccharides, plant cell walls are composed of hydroxyproline-rich glycoproteins (HRGPs), which include several groups of O-glycoproteins, including extensins (EXTs).
25655826	0	28	theme	prolyl-4-hydroxylases	22:42	arg1	regulation					8:17	Complex regulation	0:17	Complex regulation of prolyl-4-hydroxylases	0:42	Complex regulation of prolyl-4-hydroxylases impacts root hair expansion.
25655826	10	29	theme	proper	1369:1374	arg1	self-assembly					1386:1398	proper cell wall self-assembly	1369:1398	proper cell wall self-assembly	1369:1398	Taken together, our results support a model in which correct peptidyl-proline hydroxylation on EXTs, and possibly in other HRGPs, is required for proper cell wall self-assembly and hence root hair elongation in Arabidopsis thaliana.
25655826	10	30	theme	correct	1276:1282	arg1	hydroxylation					1301:1313	correct peptidyl-proline hydroxylation	1276:1313	correct peptidyl-proline hydroxylation	1276:1313	Taken together, our results support a model in which correct peptidyl-proline hydroxylation on EXTs, and possibly in other HRGPs, is required for proper cell wall self-assembly and hence root hair elongation in Arabidopsis thaliana.
25655826	7	31	contain	have	957:960	arg2	functions					978:986	interchangeable functions	962:986	interchangeable functions	962:986	Third, by P4H promoter and protein swapping approaches, we show that P4H2 and P4H13 have interchangeable functions but cannot replace P4H5.
25655826	7	31	contain	have	957:960	arg1	P4H2					942:945	P4H2	942:945	P4H2	942:945	Third, by P4H promoter and protein swapping approaches, we show that P4H2 and P4H13 have interchangeable functions but cannot replace P4H5.
25655826	7	31	contain	have	957:960	arg1	P4H13					951:955	P4H13	951:955	P4H13	951:955	Third, by P4H promoter and protein swapping approaches, we show that P4H2 and P4H13 have interchangeable functions but cannot replace P4H5.
25655826	6	32	contain	has	790:792	arg2	specificity					813:823	in vitro preferred specificity	794:823	in vitro preferred specificity for EXT substrates rather than for other HRGPs	794:870	Second, we demonstrate that P4H5 has in vitro preferred specificity for EXT substrates rather than for other HRGPs.
25655826	6	32	contain	has	790:792	arg1	P4H5					785:788	P4H5	785:788	P4H5	785:788	Second, we demonstrate that P4H5 has in vitro preferred specificity for EXT substrates rather than for other HRGPs.
25655826	3	33	theme	hydroxyproline-rich	338:356	arg1	glycoproteins					358:370	hydroxyproline-rich glycoproteins	338:370	hydroxyproline-rich glycoproteins (HRGPs)	338:378	In addition to polysaccharides, plant cell walls are composed of hydroxyproline-rich glycoproteins (HRGPs), which include several groups of O-glycoproteins, including extensins (EXTs).
25655826	3	33	theme	hydroxyproline-rich	338:356	arg1	HRGPs					373:377	HRGPs	373:377	HRGPs	373:377	In addition to polysaccharides, plant cell walls are composed of hydroxyproline-rich glycoproteins (HRGPs), which include several groups of O-glycoproteins, including extensins (EXTs).
25655826	4	34	theme	HRGPs	531:535	arg1	modification					509:520	an early post-translational modification	481:520	an early post-translational modification (PTM) of HRGPs catalyzed by prolyl 4-hydroxylases (P4Hs)	481:577	Proline hydroxylation, an early post-translational modification (PTM) of HRGPs catalyzed by prolyl 4-hydroxylases (P4Hs), defines their subsequent O-glycosylation sites.
25655826	4	34	theme	HRGPs	531:535	arg1	hydroxylation					466:478	Proline hydroxylation	458:478	Proline hydroxylation	458:478	Proline hydroxylation, an early post-translational modification (PTM) of HRGPs catalyzed by prolyl 4-hydroxylases (P4Hs), defines their subsequent O-glycosylation sites.
25655826	4	34	theme	HRGPs	531:535	arg1	PTM					523:525	PTM	523:525	PTM	523:525	Proline hydroxylation, an early post-translational modification (PTM) of HRGPs catalyzed by prolyl 4-hydroxylases (P4Hs), defines their subsequent O-glycosylation sites.
25655826	10	35	theme	hair	1415:1418	arg1	elongation					1420:1429	root hair elongation	1410:1429	root hair elongation	1410:1429	Taken together, our results support a model in which correct peptidyl-proline hydroxylation on EXTs, and possibly in other HRGPs, is required for proper cell wall self-assembly and hence root hair elongation in Arabidopsis thaliana.
25655826	0	36	theme	root	52:55	arg1	expansion					62:70	root hair expansion	52:70	root hair expansion	52:70	Complex regulation of prolyl-4-hydroxylases impacts root hair expansion.
25655826	1	37	theme	fungal	176:181	arg1	hyphae					183:188	fungal hyphae	176:188	fungal hyphae	176:188	Root hairs are single cells that develop by tip growth, a process shared with pollen tubes, axons, and fungal hyphae.
25655826	2	38	theme	structural	200:209	arg1	walls					222:226	structural plant cell walls	200:226	structural plant cell walls	200:226	However, structural plant cell walls impose constraints to accomplish tip growth.
25655826	3	39	theme	O-glycoproteins	413:427	arg1	groups					403:408	several groups	395:408	several groups	395:408	In addition to polysaccharides, plant cell walls are composed of hydroxyproline-rich glycoproteins (HRGPs), which include several groups of O-glycoproteins, including extensins (EXTs).
25655826	3	39	theme	O-glycoproteins	413:427	arg1	O-glycoproteins					413:427	O-glycoproteins	413:427	O-glycoproteins	413:427	In addition to polysaccharides, plant cell walls are composed of hydroxyproline-rich glycoproteins (HRGPs), which include several groups of O-glycoproteins, including extensins (EXTs).
25655826	3	39	theme	O-glycoproteins	413:427	arg1	extensins					440:448	extensins	440:448	extensins (EXTs)	440:455	In addition to polysaccharides, plant cell walls are composed of hydroxyproline-rich glycoproteins (HRGPs), which include several groups of O-glycoproteins, including extensins (EXTs).
25655826	4	40	theme	post-translational	490:507	arg1	modification					509:520	an early post-translational modification	481:520	an early post-translational modification (PTM) of HRGPs catalyzed by prolyl 4-hydroxylases (P4Hs)	481:577	Proline hydroxylation, an early post-translational modification (PTM) of HRGPs catalyzed by prolyl 4-hydroxylases (P4Hs), defines their subsequent O-glycosylation sites.
25655826	4	40	theme	post-translational	490:507	arg1	hydroxylation					466:478	Proline hydroxylation	458:478	Proline hydroxylation	458:478	Proline hydroxylation, an early post-translational modification (PTM) of HRGPs catalyzed by prolyl 4-hydroxylases (P4Hs), defines their subsequent O-glycosylation sites.
25655826	4	40	theme	post-translational	490:507	arg1	PTM					523:525	PTM	523:525	PTM	523:525	Proline hydroxylation, an early post-translational modification (PTM) of HRGPs catalyzed by prolyl 4-hydroxylases (P4Hs), defines their subsequent O-glycosylation sites.
25655826	7	41	theme	interchangeable	962:976	arg1	functions					978:986	interchangeable functions	962:986	interchangeable functions	962:986	Third, by P4H promoter and protein swapping approaches, we show that P4H2 and P4H13 have interchangeable functions but cannot replace P4H5.
25655826	10	42	from	elongation	1420:1429	arg1	thaliana					1446:1453	Arabidopsis thaliana	1434:1453	Arabidopsis thaliana	1434:1453	Taken together, our results support a model in which correct peptidyl-proline hydroxylation on EXTs, and possibly in other HRGPs, is required for proper cell wall self-assembly and hence root hair elongation in Arabidopsis thaliana.
25655826	5	43	theme	hair	740:743	arg1	growth					749:754	root hair tip growth	735:754	root hair tip growth	735:754	In this work, our genetic analyses prove that P4H5, and to a lesser extent P4H2 and P4H13, are pivotal for root hair tip growth.
25655826	4	44	theme	subsequent	594:603	arg1	sites					621:625	their subsequent O-glycosylation sites	588:625	their subsequent O-glycosylation sites	588:625	Proline hydroxylation, an early post-translational modification (PTM) of HRGPs catalyzed by prolyl 4-hydroxylases (P4Hs), defines their subsequent O-glycosylation sites.
25655826	4	45	theme	early	484:488	arg1	modification					509:520	an early post-translational modification	481:520	an early post-translational modification (PTM) of HRGPs catalyzed by prolyl 4-hydroxylases (P4Hs)	481:577	Proline hydroxylation, an early post-translational modification (PTM) of HRGPs catalyzed by prolyl 4-hydroxylases (P4Hs), defines their subsequent O-glycosylation sites.
25655826	4	45	theme	early	484:488	arg1	hydroxylation					466:478	Proline hydroxylation	458:478	Proline hydroxylation	458:478	Proline hydroxylation, an early post-translational modification (PTM) of HRGPs catalyzed by prolyl 4-hydroxylases (P4Hs), defines their subsequent O-glycosylation sites.
25655826	4	45	theme	early	484:488	arg1	PTM					523:525	PTM	523:525	PTM	523:525	Proline hydroxylation, an early post-translational modification (PTM) of HRGPs catalyzed by prolyl 4-hydroxylases (P4Hs), defines their subsequent O-glycosylation sites.
25655826	5	46	dep	P4H5	674:677	arg1	P4H13					712:716	P4H13	712:716	P4H13	712:716	In this work, our genetic analyses prove that P4H5, and to a lesser extent P4H2 and P4H13, are pivotal for root hair tip growth.
25655826	5	46	dep	P4H5	674:677	arg1	P4H2					703:706	P4H2	703:706	P4H2	703:706	In this work, our genetic analyses prove that P4H5, and to a lesser extent P4H2 and P4H13, are pivotal for root hair tip growth.
25655826	9	47	theme	cell	1199:1202	arg1	architecture					1209:1220	the cell wall architecture	1195:1220	the cell wall architecture	1195:1220	Finally, we explore the impact of deficient proline hydroxylation on the cell wall architecture.
25655826	0	48	theme	hair	57:60	arg1	expansion					62:70	root hair expansion	52:70	root hair expansion	52:70	Complex regulation of prolyl-4-hydroxylases impacts root hair expansion.
25655826	3	49	gly	O-glycoproteins	413:427	arg1	O-glycoproteins					413:427	O-glycoproteins	413:427	O-glycoproteins	413:427	In addition to polysaccharides, plant cell walls are composed of hydroxyproline-rich glycoproteins (HRGPs), which include several groups of O-glycoproteins, including extensins (EXTs).
25655826	7	50	theme	swapping	908:915	arg1	approaches					917:926	protein swapping approaches	900:926	protein swapping approaches	900:926	Third, by P4H promoter and protein swapping approaches, we show that P4H2 and P4H13 have interchangeable functions but cannot replace P4H5.
25655826	1	51	theme	tip	117:119	arg1	growth					121:126	tip growth	117:126	tip growth	117:126	Root hairs are single cells that develop by tip growth, a process shared with pollen tubes, axons, and fungal hyphae.
25655826	10	52	theme	wall	1381:1384	arg1	self-assembly					1386:1398	proper cell wall self-assembly	1369:1398	proper cell wall self-assembly	1369:1398	Taken together, our results support a model in which correct peptidyl-proline hydroxylation on EXTs, and possibly in other HRGPs, is required for proper cell wall self-assembly and hence root hair elongation in Arabidopsis thaliana.
25655826	5	53	theme	genetic	646:652	arg1	analyses					654:661	our genetic analyses	642:661	our genetic analyses	642:661	In this work, our genetic analyses prove that P4H5, and to a lesser extent P4H2 and P4H13, are pivotal for root hair tip growth.
25655826	5	54	theme	tip	745:747	arg1	growth					749:754	root hair tip growth	735:754	root hair tip growth	735:754	In this work, our genetic analyses prove that P4H5, and to a lesser extent P4H2 and P4H13, are pivotal for root hair tip growth.
25655826	5	55	theme	lesser	689:694	arg1	extent					696:701	a lesser extent	687:701	a lesser extent	687:701	In this work, our genetic analyses prove that P4H5, and to a lesser extent P4H2 and P4H13, are pivotal for root hair tip growth.
25655826	5	55	theme	lesser	689:694	arg1	pivotal					723:729	pivotal	723:729	pivotal	723:729	In this work, our genetic analyses prove that P4H5, and to a lesser extent P4H2 and P4H13, are pivotal for root hair tip growth.
25655826	2	56	theme	cell	217:220	arg1	walls					222:226	structural plant cell walls	200:226	structural plant cell walls	200:226	However, structural plant cell walls impose constraints to accomplish tip growth.
25655826	7	57	theme	protein	900:906	arg1	approaches					917:926	protein swapping approaches	900:926	protein swapping approaches	900:926	Third, by P4H promoter and protein swapping approaches, we show that P4H2 and P4H13 have interchangeable functions but cannot replace P4H5.
25655826	3	58	theme	plant	305:309	arg1	walls					316:320	plant cell walls	305:320	plant cell walls	305:320	In addition to polysaccharides, plant cell walls are composed of hydroxyproline-rich glycoproteins (HRGPs), which include several groups of O-glycoproteins, including extensins (EXTs).
25655826	9	59	theme	proline	1170:1176	arg1	hydroxylation					1178:1190	deficient proline hydroxylation	1160:1190	deficient proline hydroxylation	1160:1190	Finally, we explore the impact of deficient proline hydroxylation on the cell wall architecture.
25655826	2	60	theme	plant	211:215	arg1	walls					222:226	structural plant cell walls	200:226	structural plant cell walls	200:226	However, structural plant cell walls impose constraints to accomplish tip growth.
25655826	7	61	theme	P4H	883:885	arg1	promoter					887:894	P4H promoter	883:894	P4H promoter	883:894	Third, by P4H promoter and protein swapping approaches, we show that P4H2 and P4H13 have interchangeable functions but cannot replace P4H5.
25655826	6	62	theme	other	860:864	arg1	HRGPs					866:870	other HRGPs	860:870	other HRGPs	860:870	Second, we demonstrate that P4H5 has in vitro preferred specificity for EXT substrates rather than for other HRGPs.
26452179	5	0	theme	Sphingobacterium	733:748	arg1	sp					750:751	bacterium Sphingobacterium sp	723:751	bacterium Sphingobacterium sp	723:751	The saccharification of rice straw co-treated with bacterium Sphingobacterium sp.
26452179	4	1	theme	mild	504:507	arg1	conditions					509:518	mild conditions	504:518	mild conditions for the sequential NU treatment	504:550	LD-1 achieved mild conditions for the sequential NU treatment, reducing the concentration of the NU solution from 7%/12% to 4%/6% and increasing the temperature from -20°C to -10°C.
26452179	3	2	with	treatment	442:450	arg1	sp					486:487	a bacterium Sphingobacterium sp	457:487	a bacterium Sphingobacterium sp	457:487	The biological treatment with a bacterium Sphingobacterium sp.
26452179	1	3	theme	combination	161:171	arg1	pretreatment					145:156	A stepwise pretreatment	134:156	A stepwise pretreatment of combination of bacterial treatment with NaOH/Urea (NU) treatment	134:224	A stepwise pretreatment of combination of bacterial treatment with NaOH/Urea (NU) treatment was conducted to enhance enzymatic hydrolysis of rice straw (RS).
26452179	1	4	theme	enzymatic	251:259	arg1	hydrolysis					261:270	enzymatic hydrolysis	251:270	enzymatic hydrolysis of rice straw (RS)	251:289	A stepwise pretreatment of combination of bacterial treatment with NaOH/Urea (NU) treatment was conducted to enhance enzymatic hydrolysis of rice straw (RS).
26452179	6	5	theme	yield	858:862	arg1	increase					819:826	1.396-fold and 1.372-fold increase	793:826	1.396-fold and 1.372-fold increase of reducing sugar and glucose yield	793:862	LD-1 and 4%/6% NU at -10°C resulted in 1.396-fold and 1.372-fold increase of reducing sugar and glucose yield respectively than that of sole NU treatment.
26452179	2	6	theme	RS	335:336	arg1	composition					320:330	the composition	316:330	the composition of RS	316:336	The results showed that the composition of RS changed significantly, the lignin and hemicellulose decreased while cellulose increased.
26452179	0	7	from	Combination	0:10	arg1	temperature					75:85	cold temperature	70:85	cold temperature	70:85	Combination of biological pretreatment with NaOH/Urea pretreatment at cold temperature to enhance enzymatic hydrolysis of rice straw.
26452179	6	8	theme	%	767:767	arg1	NU					769:770	LD-1 and 4%/6% NU	754:770	LD-1 and 4%/6% NU at -10°C	754:779	LD-1 and 4%/6% NU at -10°C resulted in 1.396-fold and 1.372-fold increase of reducing sugar and glucose yield respectively than that of sole NU treatment.
26452179	0	9	theme	enzymatic	98:106	arg1	hydrolysis					108:117	enzymatic hydrolysis	98:117	enzymatic hydrolysis of rice straw	98:131	Combination of biological pretreatment with NaOH/Urea pretreatment at cold temperature to enhance enzymatic hydrolysis of rice straw.
26452179	6	10	theme	/6	765:766	arg1	%					767:767	4%/6%	763:767	4%/6%	763:767	LD-1 and 4%/6% NU at -10°C resulted in 1.396-fold and 1.372-fold increase of reducing sugar and glucose yield respectively than that of sole NU treatment.
26452179	1	11	theme	rice	275:278	arg1	RS					287:288	RS	287:288	RS	287:288	A stepwise pretreatment of combination of bacterial treatment with NaOH/Urea (NU) treatment was conducted to enhance enzymatic hydrolysis of rice straw (RS).
26452179	1	11	theme	rice	275:278	arg1	straw					280:284	rice straw	275:284	rice straw (RS)	275:289	A stepwise pretreatment of combination of bacterial treatment with NaOH/Urea (NU) treatment was conducted to enhance enzymatic hydrolysis of rice straw (RS).
26452179	0	12	theme	rice	122:125	arg1	straw					127:131	rice straw	122:131	rice straw	122:131	Combination of biological pretreatment with NaOH/Urea pretreatment at cold temperature to enhance enzymatic hydrolysis of rice straw.
26452179	6	13	theme	sugar	840:844	arg1	increase					819:826	1.396-fold and 1.372-fold increase	793:826	1.396-fold and 1.372-fold increase of reducing sugar and glucose yield	793:862	LD-1 and 4%/6% NU at -10°C resulted in 1.396-fold and 1.372-fold increase of reducing sugar and glucose yield respectively than that of sole NU treatment.
26452179	3	14	theme	Sphingobacterium	469:484	arg1	sp					486:487	a bacterium Sphingobacterium sp	457:487	a bacterium Sphingobacterium sp	457:487	The biological treatment with a bacterium Sphingobacterium sp.
26452179	1	15	theme	bacterial	176:184	arg1	treatment					186:194	bacterial treatment	176:194	bacterial treatment with NaOH/Urea (NU) treatment	176:224	A stepwise pretreatment of combination of bacterial treatment with NaOH/Urea (NU) treatment was conducted to enhance enzymatic hydrolysis of rice straw (RS).
26452179	4	16	theme	solution	590:597	arg1	concentration					566:578	the concentration	562:578	the concentration of the NU solution	562:597	LD-1 achieved mild conditions for the sequential NU treatment, reducing the concentration of the NU solution from 7%/12% to 4%/6% and increasing the temperature from -20°C to -10°C.
26452179	6	17	theme	reducing	831:838	arg1	sugar					840:844	reducing sugar	831:844	reducing sugar	831:844	LD-1 and 4%/6% NU at -10°C resulted in 1.396-fold and 1.372-fold increase of reducing sugar and glucose yield respectively than that of sole NU treatment.
26452179	4	18	theme	NU	587:588	arg1	solution					590:597	the NU solution	583:597	the NU solution	583:597	LD-1 achieved mild conditions for the sequential NU treatment, reducing the concentration of the NU solution from 7%/12% to 4%/6% and increasing the temperature from -20°C to -10°C.
26452179	6	19	theme	1.396-fold	793:802	arg1	increase					819:826	1.396-fold and 1.372-fold increase	793:826	1.396-fold and 1.372-fold increase of reducing sugar and glucose yield	793:862	LD-1 and 4%/6% NU at -10°C resulted in 1.396-fold and 1.372-fold increase of reducing sugar and glucose yield respectively than that of sole NU treatment.
26452179	1	20	theme	treatment	186:194	arg1	combination					161:171	combination	161:171	combination of bacterial treatment with NaOH/Urea (NU) treatment	161:224	A stepwise pretreatment of combination of bacterial treatment with NaOH/Urea (NU) treatment was conducted to enhance enzymatic hydrolysis of rice straw (RS).
26452179	4	21	dep	achieved	495:502	arg1	increasing					624:633	increasing	624:633	increasing the temperature from -20°C to -10°C	624:669	LD-1 achieved mild conditions for the sequential NU treatment, reducing the concentration of the NU solution from 7%/12% to 4%/6% and increasing the temperature from -20°C to -10°C.
26452179	4	21	dep	achieved	495:502	arg1	reducing					553:560	reducing	553:560	reducing the concentration of the NU solution from 7%/12% to 4%/6%	553:618	LD-1 achieved mild conditions for the sequential NU treatment, reducing the concentration of the NU solution from 7%/12% to 4%/6% and increasing the temperature from -20°C to -10°C.
26452179	1	22	theme	straw	280:284	arg1	hydrolysis					261:270	enzymatic hydrolysis	251:270	enzymatic hydrolysis of rice straw (RS)	251:289	A stepwise pretreatment of combination of bacterial treatment with NaOH/Urea (NU) treatment was conducted to enhance enzymatic hydrolysis of rice straw (RS).
26452179	6	23	theme	LD-1	754:757	arg1	NU					769:770	LD-1 and 4%/6% NU	754:770	LD-1 and 4%/6% NU at -10°C	754:779	LD-1 and 4%/6% NU at -10°C resulted in 1.396-fold and 1.372-fold increase of reducing sugar and glucose yield respectively than that of sole NU treatment.
26452179	1	24	theme	NaOH/Urea	201:209	arg1	treatment					216:224	NaOH/Urea (NU) treatment	201:224	NaOH/Urea (NU) treatment	201:224	A stepwise pretreatment of combination of bacterial treatment with NaOH/Urea (NU) treatment was conducted to enhance enzymatic hydrolysis of rice straw (RS).
26452179	0	25	theme	pretreatment	26:37	arg1	Combination					0:10	Combination	0:10	Combination of biological pretreatment with NaOH/Urea pretreatment at cold temperature	0:85	Combination of biological pretreatment with NaOH/Urea pretreatment at cold temperature to enhance enzymatic hydrolysis of rice straw.
26452179	6	26	theme	%	764:764	arg1	%					767:767	4%/6%	763:767	4%/6%	763:767	LD-1 and 4%/6% NU at -10°C resulted in 1.396-fold and 1.372-fold increase of reducing sugar and glucose yield respectively than that of sole NU treatment.
26452179	3	27	theme	bacterium	459:467	arg1	sp					486:487	a bacterium Sphingobacterium sp	457:487	a bacterium Sphingobacterium sp	457:487	The biological treatment with a bacterium Sphingobacterium sp.
26452179	0	28	theme	biological	15:24	arg1	pretreatment					26:37	biological pretreatment	15:37	biological pretreatment with NaOH/Urea pretreatment at cold temperature	15:85	Combination of biological pretreatment with NaOH/Urea pretreatment at cold temperature to enhance enzymatic hydrolysis of rice straw.
26452179	0	29	theme	straw	127:131	arg1	hydrolysis					108:117	enzymatic hydrolysis	98:117	enzymatic hydrolysis of rice straw	98:131	Combination of biological pretreatment with NaOH/Urea pretreatment at cold temperature to enhance enzymatic hydrolysis of rice straw.
26452179	4	30	dep	-10°C	665:669	arg1	to					662:663	to	662:663	to	662:663	LD-1 achieved mild conditions for the sequential NU treatment, reducing the concentration of the NU solution from 7%/12% to 4%/6% and increasing the temperature from -20°C to -10°C.
26452179	1	31	theme	NU	212:213	arg1	treatment					216:224	NaOH/Urea (NU) treatment	201:224	NaOH/Urea (NU) treatment	201:224	A stepwise pretreatment of combination of bacterial treatment with NaOH/Urea (NU) treatment was conducted to enhance enzymatic hydrolysis of rice straw (RS).
26452179	0	32	theme	NaOH/Urea	44:52	arg1	pretreatment					54:65	NaOH/Urea pretreatment	44:65	NaOH/Urea pretreatment	44:65	Combination of biological pretreatment with NaOH/Urea pretreatment at cold temperature to enhance enzymatic hydrolysis of rice straw.
26452179	6	33	theme	1.372-fold	808:817	arg1	increase					819:826	1.396-fold and 1.372-fold increase	793:826	1.396-fold and 1.372-fold increase of reducing sugar and glucose yield	793:862	LD-1 and 4%/6% NU at -10°C resulted in 1.396-fold and 1.372-fold increase of reducing sugar and glucose yield respectively than that of sole NU treatment.
26452179	4	34	theme	/6	616:617	arg1	%					618:618	7%/12% to 4%/6%	604:618	7%/12% to 4%/6%	604:618	LD-1 achieved mild conditions for the sequential NU treatment, reducing the concentration of the NU solution from 7%/12% to 4%/6% and increasing the temperature from -20°C to -10°C.
26452179	3	35	theme	biological	431:440	arg1	treatment					442:450	The biological treatment	427:450	The biological treatment with a bacterium Sphingobacterium sp	427:487	The biological treatment with a bacterium Sphingobacterium sp.
26452179	4	36	theme	NU	539:540	arg1	treatment					542:550	the sequential NU treatment	524:550	the sequential NU treatment	524:550	LD-1 achieved mild conditions for the sequential NU treatment, reducing the concentration of the NU solution from 7%/12% to 4%/6% and increasing the temperature from -20°C to -10°C.
26452179	0	37	from	temperature	75:85	arg1	Combination					0:10	Combination	0:10	Combination of biological pretreatment with NaOH/Urea pretreatment at cold temperature	0:85	Combination of biological pretreatment with NaOH/Urea pretreatment at cold temperature to enhance enzymatic hydrolysis of rice straw.
26452179	0	37	from	temperature	75:85	arg1	pretreatment					26:37	biological pretreatment	15:37	biological pretreatment with NaOH/Urea pretreatment at cold temperature	15:85	Combination of biological pretreatment with NaOH/Urea pretreatment at cold temperature to enhance enzymatic hydrolysis of rice straw.
26452179	4	38	dep	%	615:615	arg1	to					611:612	to	611:612	to	611:612	LD-1 achieved mild conditions for the sequential NU treatment, reducing the concentration of the NU solution from 7%/12% to 4%/6% and increasing the temperature from -20°C to -10°C.
26452179	0	39	with	pretreatment	26:37	arg1	pretreatment					54:65	NaOH/Urea pretreatment	44:65	NaOH/Urea pretreatment	44:65	Combination of biological pretreatment with NaOH/Urea pretreatment at cold temperature to enhance enzymatic hydrolysis of rice straw.
26452179	4	40	theme	sequential	528:537	arg1	treatment					542:550	the sequential NU treatment	524:550	the sequential NU treatment	524:550	LD-1 achieved mild conditions for the sequential NU treatment, reducing the concentration of the NU solution from 7%/12% to 4%/6% and increasing the temperature from -20°C to -10°C.
26452179	5	41	theme	bacterium	723:731	arg1	sp					750:751	bacterium Sphingobacterium sp	723:751	bacterium Sphingobacterium sp	723:751	The saccharification of rice straw co-treated with bacterium Sphingobacterium sp.
26452179	6	42	theme	glucose	850:856	arg1	yield					858:862	glucose yield	850:862	glucose yield	850:862	LD-1 and 4%/6% NU at -10°C resulted in 1.396-fold and 1.372-fold increase of reducing sugar and glucose yield respectively than that of sole NU treatment.
26452179	5	43	dep	co-treated	707:716	arg1	saccharification					676:691	The saccharification	672:691	The saccharification of rice straw	672:705	The saccharification of rice straw co-treated with bacterium Sphingobacterium sp.
26452179	6	44	theme	NU	895:896	arg1	treatment					898:906	sole NU treatment	890:906	sole NU treatment	890:906	LD-1 and 4%/6% NU at -10°C resulted in 1.396-fold and 1.372-fold increase of reducing sugar and glucose yield respectively than that of sole NU treatment.
26452179	5	45	with	co-treated	707:716	arg1	sp					750:751	bacterium Sphingobacterium sp	723:751	bacterium Sphingobacterium sp	723:751	The saccharification of rice straw co-treated with bacterium Sphingobacterium sp.
26452179	4	46	theme	%	605:605	arg1	%					615:615	7%/12% to 4%	604:615	7%/12% to 4%	604:615	LD-1 achieved mild conditions for the sequential NU treatment, reducing the concentration of the NU solution from 7%/12% to 4%/6% and increasing the temperature from -20°C to -10°C.
26452179	1	47	with	treatment	186:194	arg1	treatment					216:224	NaOH/Urea (NU) treatment	201:224	NaOH/Urea (NU) treatment	201:224	A stepwise pretreatment of combination of bacterial treatment with NaOH/Urea (NU) treatment was conducted to enhance enzymatic hydrolysis of rice straw (RS).
26452179	1	48	theme	stepwise	136:143	arg1	pretreatment					145:156	A stepwise pretreatment	134:156	A stepwise pretreatment of combination of bacterial treatment with NaOH/Urea (NU) treatment	134:224	A stepwise pretreatment of combination of bacterial treatment with NaOH/Urea (NU) treatment was conducted to enhance enzymatic hydrolysis of rice straw (RS).
26452179	5	49	theme	straw	701:705	arg1	saccharification					676:691	The saccharification	672:691	The saccharification of rice straw	672:705	The saccharification of rice straw co-treated with bacterium Sphingobacterium sp.
26452179	4	50	from	-10°C	665:669	arg1	temperature					639:649	the temperature	635:649	the temperature from -20°C to -10°C	635:669	LD-1 achieved mild conditions for the sequential NU treatment, reducing the concentration of the NU solution from 7%/12% to 4%/6% and increasing the temperature from -20°C to -10°C.
26452179	4	51	theme	%	609:609	arg1	%					615:615	7%/12% to 4%	604:615	7%/12% to 4%	604:615	LD-1 achieved mild conditions for the sequential NU treatment, reducing the concentration of the NU solution from 7%/12% to 4%/6% and increasing the temperature from -20°C to -10°C.
26452179	0	52	theme	cold	70:73	arg1	temperature					75:85	cold temperature	70:85	cold temperature	70:85	Combination of biological pretreatment with NaOH/Urea pretreatment at cold temperature to enhance enzymatic hydrolysis of rice straw.
26452179	6	53	from	-10°C	775:779	arg1	NU					769:770	LD-1 and 4%/6% NU	754:770	LD-1 and 4%/6% NU at -10°C	754:779	LD-1 and 4%/6% NU at -10°C resulted in 1.396-fold and 1.372-fold increase of reducing sugar and glucose yield respectively than that of sole NU treatment.
26452179	6	54	theme	sole	890:893	arg1	treatment					898:906	sole NU treatment	890:906	sole NU treatment	890:906	LD-1 and 4%/6% NU at -10°C resulted in 1.396-fold and 1.372-fold increase of reducing sugar and glucose yield respectively than that of sole NU treatment.
26452179	5	55	theme	rice	696:699	arg1	straw					701:705	rice straw	696:705	rice straw	696:705	The saccharification of rice straw co-treated with bacterium Sphingobacterium sp.
27417558	3	0	from	increase	623:630	arg1	concentration					654:666	the polysaccharide concentration	635:666	the polysaccharide concentration	635:666	RESULTS We determined that the addition of inorganic nitrogen promoted nitrogen liberation to the extracellular medium, while the addition of inactive dry yeast to the base wine caused an increase in the polysaccharide concentration and foaming properties of the sparkling wine.
27417558	1	1	dep	BACKGROUND	83:92	arg1	formation					151:159	the formation	147:159	the formation	147:159	BACKGROUND The differential characteristic of sparkling wine is the formation of foam, which is dependent, among other factors, on yeast autolysis, aging and oenological practices.
27417558	1	1	dep	BACKGROUND	83:92	arg1	dependent					179:187	dependent	179:187	dependent	179:187	BACKGROUND The differential characteristic of sparkling wine is the formation of foam, which is dependent, among other factors, on yeast autolysis, aging and oenological practices.
27417558	1	1	dep	BACKGROUND	83:92	arg1	characteristic					111:124	The differential characteristic	94:124	The differential characteristic of sparkling wine	94:142	BACKGROUND The differential characteristic of sparkling wine is the formation of foam, which is dependent, among other factors, on yeast autolysis, aging and oenological practices.
27417558	4	2	from	content	936:942	arg1	wine					959:962	the natural wine	947:962	the natural wine	947:962	The use of synthetic and natural base wines allowed us to discriminate that the differences in high-molecular-weight polysaccharides and oligosaccharides could be attributed to the yeast cells and that the higher nitrogen content in the natural wine could be due to external proteolysis.
27417558	4	3	theme	natural	951:957	arg1	wine					959:962	the natural wine	947:962	the natural wine	947:962	The use of synthetic and natural base wines allowed us to discriminate that the differences in high-molecular-weight polysaccharides and oligosaccharides could be attributed to the yeast cells and that the higher nitrogen content in the natural wine could be due to external proteolysis.
27417558	5	4	theme	main	1109:1112	arg1	characteristics					1114:1128	the main characteristics	1105:1128	the main characteristics of the sparkling wine	1105:1150	CONCLUSION The practices of nitrogen addition and supplementation of inactive dry yeast could modulate the main characteristics of the sparkling wine and be a critical element for the design of this kind of wine.
27417558	6	5	theme	Chemical	1233:1240	arg1	Industry					1242:1249	Chemical Industry	1233:1249	Chemical Industry	1233:1249	© 2016 Society of Chemical Industry.
27417558	2	6	from	effects	295:301	arg1	composition					402:412	the sparkling wine composition	383:412	the sparkling wine composition	383:412	In this study, we analyzed the effects of yeast strain, nutrient supplementation to the base wine and aging process on the sparkling wine composition and its foamability.
27417558	2	6	from	effects	295:301	arg1	foamability					422:432	its foamability	418:432	its foamability	418:432	In this study, we analyzed the effects of yeast strain, nutrient supplementation to the base wine and aging process on the sparkling wine composition and its foamability.
27417558	1	7	theme	foam	164:167	arg1	formation					151:159	the formation	147:159	the formation	147:159	BACKGROUND The differential characteristic of sparkling wine is the formation of foam, which is dependent, among other factors, on yeast autolysis, aging and oenological practices.
27417558	1	7	theme	foam	164:167	arg1	dependent					179:187	dependent	179:187	dependent	179:187	BACKGROUND The differential characteristic of sparkling wine is the formation of foam, which is dependent, among other factors, on yeast autolysis, aging and oenological practices.
27417558	1	7	theme	foam	164:167	arg1	characteristic					111:124	The differential characteristic	94:124	The differential characteristic of sparkling wine	94:142	BACKGROUND The differential characteristic of sparkling wine is the formation of foam, which is dependent, among other factors, on yeast autolysis, aging and oenological practices.
27417558	5	8	theme	addition	1039:1046	arg1	supplementation					1052:1066	supplementation	1052:1066	supplementation of inactive dry yeast	1052:1088	CONCLUSION The practices of nitrogen addition and supplementation of inactive dry yeast could modulate the main characteristics of the sparkling wine and be a critical element for the design of this kind of wine.
27417558	5	8	theme	addition	1039:1046	arg1	practices					1017:1025	The practices	1013:1025	The practices of nitrogen addition	1013:1046	CONCLUSION The practices of nitrogen addition and supplementation of inactive dry yeast could modulate the main characteristics of the sparkling wine and be a critical element for the design of this kind of wine.
27417558	4	9	theme	higher	920:925	arg1	due					973:975	due	973:975	due	973:975	The use of synthetic and natural base wines allowed us to discriminate that the differences in high-molecular-weight polysaccharides and oligosaccharides could be attributed to the yeast cells and that the higher nitrogen content in the natural wine could be due to external proteolysis.
27417558	4	9	theme	higher	920:925	arg1	content					936:942	the higher nitrogen content	916:942	the higher nitrogen content in the natural wine	916:962	The use of synthetic and natural base wines allowed us to discriminate that the differences in high-molecular-weight polysaccharides and oligosaccharides could be attributed to the yeast cells and that the higher nitrogen content in the natural wine could be due to external proteolysis.
27417558	3	10	theme	polysaccharide	639:652	arg1	concentration					654:666	the polysaccharide concentration	635:666	the polysaccharide concentration	635:666	RESULTS We determined that the addition of inorganic nitrogen promoted nitrogen liberation to the extracellular medium, while the addition of inactive dry yeast to the base wine caused an increase in the polysaccharide concentration and foaming properties of the sparkling wine.
27417558	3	11	theme	nitrogen	506:513	arg1	liberation					515:524	nitrogen liberation	506:524	nitrogen liberation	506:524	RESULTS We determined that the addition of inorganic nitrogen promoted nitrogen liberation to the extracellular medium, while the addition of inactive dry yeast to the base wine caused an increase in the polysaccharide concentration and foaming properties of the sparkling wine.
27417558	5	12	theme	inactive	1071:1078	arg1	yeast					1084:1088	inactive dry yeast	1071:1088	inactive dry yeast	1071:1088	CONCLUSION The practices of nitrogen addition and supplementation of inactive dry yeast could modulate the main characteristics of the sparkling wine and be a critical element for the design of this kind of wine.
27417558	1	13	theme	oenological	241:251	arg1	practices					253:261	oenological practices	241:261	oenological practices	241:261	BACKGROUND The differential characteristic of sparkling wine is the formation of foam, which is dependent, among other factors, on yeast autolysis, aging and oenological practices.
27417558	5	14	theme	dry	1080:1082	arg1	yeast					1084:1088	inactive dry yeast	1071:1088	inactive dry yeast	1071:1088	CONCLUSION The practices of nitrogen addition and supplementation of inactive dry yeast could modulate the main characteristics of the sparkling wine and be a critical element for the design of this kind of wine.
27417558	3	15	theme	nitrogen	488:495	arg1	addition					466:473	the addition	462:473	the addition of inorganic nitrogen	462:495	RESULTS We determined that the addition of inorganic nitrogen promoted nitrogen liberation to the extracellular medium, while the addition of inactive dry yeast to the base wine caused an increase in the polysaccharide concentration and foaming properties of the sparkling wine.
27417558	4	16	theme	natural	739:745	arg1	wines					752:756	synthetic and natural base wines	725:756	synthetic and natural base wines	725:756	The use of synthetic and natural base wines allowed us to discriminate that the differences in high-molecular-weight polysaccharides and oligosaccharides could be attributed to the yeast cells and that the higher nitrogen content in the natural wine could be due to external proteolysis.
27417558	4	17	theme	nitrogen	927:934	arg1	due					973:975	due	973:975	due	973:975	The use of synthetic and natural base wines allowed us to discriminate that the differences in high-molecular-weight polysaccharides and oligosaccharides could be attributed to the yeast cells and that the higher nitrogen content in the natural wine could be due to external proteolysis.
27417558	4	17	theme	nitrogen	927:934	arg1	content					936:942	the higher nitrogen content	916:942	the higher nitrogen content in the natural wine	916:962	The use of synthetic and natural base wines allowed us to discriminate that the differences in high-molecular-weight polysaccharides and oligosaccharides could be attributed to the yeast cells and that the higher nitrogen content in the natural wine could be due to external proteolysis.
27417558	3	18	theme	foaming	672:678	arg1	properties					680:689	foaming properties	672:689	foaming properties of the sparkling wine	672:711	RESULTS We determined that the addition of inorganic nitrogen promoted nitrogen liberation to the extracellular medium, while the addition of inactive dry yeast to the base wine caused an increase in the polysaccharide concentration and foaming properties of the sparkling wine.
27417558	3	19	from	properties	680:689	arg1	concentration					654:666	the polysaccharide concentration	635:666	the polysaccharide concentration	635:666	RESULTS We determined that the addition of inorganic nitrogen promoted nitrogen liberation to the extracellular medium, while the addition of inactive dry yeast to the base wine caused an increase in the polysaccharide concentration and foaming properties of the sparkling wine.
27417558	2	20	theme	base	352:355	arg1	wine					357:360	the base wine	348:360	the base wine	348:360	In this study, we analyzed the effects of yeast strain, nutrient supplementation to the base wine and aging process on the sparkling wine composition and its foamability.
27417558	5	21	theme	critical	1161:1168	arg1	element					1170:1176	a critical element	1159:1176	a critical element for the design of this kind of wine	1159:1212	CONCLUSION The practices of nitrogen addition and supplementation of inactive dry yeast could modulate the main characteristics of the sparkling wine and be a critical element for the design of this kind of wine.
27417558	4	22	theme	high-molecular-weight	809:829	arg1	polysaccharides					831:845	high-molecular-weight polysaccharides	809:845	high-molecular-weight polysaccharides	809:845	The use of synthetic and natural base wines allowed us to discriminate that the differences in high-molecular-weight polysaccharides and oligosaccharides could be attributed to the yeast cells and that the higher nitrogen content in the natural wine could be due to external proteolysis.
27417558	3	23	theme	extracellular	533:545	arg1	medium					547:552	the extracellular medium	529:552	the extracellular medium	529:552	RESULTS We determined that the addition of inorganic nitrogen promoted nitrogen liberation to the extracellular medium, while the addition of inactive dry yeast to the base wine caused an increase in the polysaccharide concentration and foaming properties of the sparkling wine.
27417558	2	24	theme	wine	397:400	arg1	composition					402:412	the sparkling wine composition	383:412	the sparkling wine composition	383:412	In this study, we analyzed the effects of yeast strain, nutrient supplementation to the base wine and aging process on the sparkling wine composition and its foamability.
27417558	4	25	theme	external	980:987	arg1	proteolysis					989:999	external proteolysis	980:999	external proteolysis	980:999	The use of synthetic and natural base wines allowed us to discriminate that the differences in high-molecular-weight polysaccharides and oligosaccharides could be attributed to the yeast cells and that the higher nitrogen content in the natural wine could be due to external proteolysis.
27417558	2	26	theme	sparkling	387:395	arg1	composition					402:412	the sparkling wine composition	383:412	the sparkling wine composition	383:412	In this study, we analyzed the effects of yeast strain, nutrient supplementation to the base wine and aging process on the sparkling wine composition and its foamability.
27417558	1	27	theme	differential	98:109	arg1	formation					151:159	the formation	147:159	the formation	147:159	BACKGROUND The differential characteristic of sparkling wine is the formation of foam, which is dependent, among other factors, on yeast autolysis, aging and oenological practices.
27417558	1	27	theme	differential	98:109	arg1	dependent					179:187	dependent	179:187	dependent	179:187	BACKGROUND The differential characteristic of sparkling wine is the formation of foam, which is dependent, among other factors, on yeast autolysis, aging and oenological practices.
27417558	1	27	theme	differential	98:109	arg1	characteristic					111:124	The differential characteristic	94:124	The differential characteristic of sparkling wine	94:142	BACKGROUND The differential characteristic of sparkling wine is the formation of foam, which is dependent, among other factors, on yeast autolysis, aging and oenological practices.
27417558	0	28	theme	yeast	16:20	arg1	Contribution					0:11	Contribution	0:11	Contribution of yeast and base wine supplementation to sparkling wine composition	0:80	Contribution of yeast and base wine supplementation to sparkling wine composition.
27417558	3	29	theme	sparkling	698:706	arg1	wine					708:711	the sparkling wine	694:711	the sparkling wine	694:711	RESULTS We determined that the addition of inorganic nitrogen promoted nitrogen liberation to the extracellular medium, while the addition of inactive dry yeast to the base wine caused an increase in the polysaccharide concentration and foaming properties of the sparkling wine.
27417558	4	30	theme	yeast	895:899	arg1	cells					901:905	the yeast cells	891:905	the yeast cells	891:905	The use of synthetic and natural base wines allowed us to discriminate that the differences in high-molecular-weight polysaccharides and oligosaccharides could be attributed to the yeast cells and that the higher nitrogen content in the natural wine could be due to external proteolysis.
27417558	3	31	theme	base	603:606	arg1	wine					608:611	the base wine	599:611	the base wine	599:611	RESULTS We determined that the addition of inorganic nitrogen promoted nitrogen liberation to the extracellular medium, while the addition of inactive dry yeast to the base wine caused an increase in the polysaccharide concentration and foaming properties of the sparkling wine.
27417558	2	32	theme	strain	312:317	arg1	effects					295:301	the effects	291:301	the effects of yeast strain, nutrient supplementation to the base wine and aging process on the sparkling wine composition and its foamability	291:432	In this study, we analyzed the effects of yeast strain, nutrient supplementation to the base wine and aging process on the sparkling wine composition and its foamability.
27417558	2	33	theme	process	372:378	arg1	effects					295:301	the effects	291:301	the effects of yeast strain, nutrient supplementation to the base wine and aging process on the sparkling wine composition and its foamability	291:432	In this study, we analyzed the effects of yeast strain, nutrient supplementation to the base wine and aging process on the sparkling wine composition and its foamability.
27417558	0	34	theme	wine	31:34	arg1	supplementation					36:50	base wine supplementation	26:50	base wine supplementation	26:50	Contribution of yeast and base wine supplementation to sparkling wine composition.
27417558	3	35	theme	wine	708:711	arg1	properties					680:689	foaming properties	672:689	foaming properties of the sparkling wine	672:711	RESULTS We determined that the addition of inorganic nitrogen promoted nitrogen liberation to the extracellular medium, while the addition of inactive dry yeast to the base wine caused an increase in the polysaccharide concentration and foaming properties of the sparkling wine.
27417558	3	35	theme	wine	708:711	arg1	increase					623:630	an increase	620:630	an increase in the polysaccharide concentration	620:666	RESULTS We determined that the addition of inorganic nitrogen promoted nitrogen liberation to the extracellular medium, while the addition of inactive dry yeast to the base wine caused an increase in the polysaccharide concentration and foaming properties of the sparkling wine.
27417558	1	36	theme	other	196:200	arg1	factors					202:208	other factors	196:208	other factors	196:208	BACKGROUND The differential characteristic of sparkling wine is the formation of foam, which is dependent, among other factors, on yeast autolysis, aging and oenological practices.
27417558	2	37	theme	aging	366:370	arg1	process					372:378	aging process	366:378	aging process	366:378	In this study, we analyzed the effects of yeast strain, nutrient supplementation to the base wine and aging process on the sparkling wine composition and its foamability.
27417558	0	38	theme	base	26:29	arg1	supplementation					36:50	base wine supplementation	26:50	base wine supplementation	26:50	Contribution of yeast and base wine supplementation to sparkling wine composition.
27417558	5	39	theme	kind	1201:1204	arg1	design					1186:1191	the design	1182:1191	the design of this kind of wine	1182:1212	CONCLUSION The practices of nitrogen addition and supplementation of inactive dry yeast could modulate the main characteristics of the sparkling wine and be a critical element for the design of this kind of wine.
27417558	5	40	theme	wine	1147:1150	arg1	characteristics					1114:1128	the main characteristics	1105:1128	the main characteristics of the sparkling wine	1105:1150	CONCLUSION The practices of nitrogen addition and supplementation of inactive dry yeast could modulate the main characteristics of the sparkling wine and be a critical element for the design of this kind of wine.
27417558	4	41	from	differences	794:804	arg1	oligosaccharides					851:866	oligosaccharides	851:866	oligosaccharides	851:866	The use of synthetic and natural base wines allowed us to discriminate that the differences in high-molecular-weight polysaccharides and oligosaccharides could be attributed to the yeast cells and that the higher nitrogen content in the natural wine could be due to external proteolysis.
27417558	4	41	from	differences	794:804	arg1	polysaccharides					831:845	high-molecular-weight polysaccharides	809:845	high-molecular-weight polysaccharides	809:845	The use of synthetic and natural base wines allowed us to discriminate that the differences in high-molecular-weight polysaccharides and oligosaccharides could be attributed to the yeast cells and that the higher nitrogen content in the natural wine could be due to external proteolysis.
27417558	2	42	theme	yeast	306:310	arg1	strain					312:317	yeast strain	306:317	yeast strain	306:317	In this study, we analyzed the effects of yeast strain, nutrient supplementation to the base wine and aging process on the sparkling wine composition and its foamability.
27417558	0	43	theme	supplementation	36:50	arg1	Contribution					0:11	Contribution	0:11	Contribution of yeast and base wine supplementation to sparkling wine composition	0:80	Contribution of yeast and base wine supplementation to sparkling wine composition.
27417558	5	44	theme	sparkling	1137:1145	arg1	wine					1147:1150	the sparkling wine	1133:1150	the sparkling wine	1133:1150	CONCLUSION The practices of nitrogen addition and supplementation of inactive dry yeast could modulate the main characteristics of the sparkling wine and be a critical element for the design of this kind of wine.
27417558	3	45	dep	RESULTS	435:441	arg1	determined					446:455	determined	446:455	determined that the addition of inorganic nitrogen promoted nitrogen liberation to the extracellular medium, while the addition of inactive dry yeast to the base wine caused an increase in the polysaccharide concentration and foaming properties of the sparkling wine	446:711	RESULTS We determined that the addition of inorganic nitrogen promoted nitrogen liberation to the extracellular medium, while the addition of inactive dry yeast to the base wine caused an increase in the polysaccharide concentration and foaming properties of the sparkling wine.
27417558	1	46	theme	sparkling	129:137	arg1	wine					139:142	sparkling wine	129:142	sparkling wine	129:142	BACKGROUND The differential characteristic of sparkling wine is the formation of foam, which is dependent, among other factors, on yeast autolysis, aging and oenological practices.
27417558	4	47	theme	wines	752:756	arg1	use					718:720	The use	714:720	The use of synthetic and natural base wines	714:756	The use of synthetic and natural base wines allowed us to discriminate that the differences in high-molecular-weight polysaccharides and oligosaccharides could be attributed to the yeast cells and that the higher nitrogen content in the natural wine could be due to external proteolysis.
27417558	4	48	theme	base	747:750	arg1	wines					752:756	synthetic and natural base wines	725:756	synthetic and natural base wines	725:756	The use of synthetic and natural base wines allowed us to discriminate that the differences in high-molecular-weight polysaccharides and oligosaccharides could be attributed to the yeast cells and that the higher nitrogen content in the natural wine could be due to external proteolysis.
27417558	3	49	theme	dry	586:588	arg1	yeast					590:594	inactive dry yeast	577:594	inactive dry yeast	577:594	RESULTS We determined that the addition of inorganic nitrogen promoted nitrogen liberation to the extracellular medium, while the addition of inactive dry yeast to the base wine caused an increase in the polysaccharide concentration and foaming properties of the sparkling wine.
27417558	1	50	theme	wine	139:142	arg1	formation					151:159	the formation	147:159	the formation	147:159	BACKGROUND The differential characteristic of sparkling wine is the formation of foam, which is dependent, among other factors, on yeast autolysis, aging and oenological practices.
27417558	1	50	theme	wine	139:142	arg1	dependent					179:187	dependent	179:187	dependent	179:187	BACKGROUND The differential characteristic of sparkling wine is the formation of foam, which is dependent, among other factors, on yeast autolysis, aging and oenological practices.
27417558	1	50	theme	wine	139:142	arg1	characteristic					111:124	The differential characteristic	94:124	The differential characteristic of sparkling wine	94:142	BACKGROUND The differential characteristic of sparkling wine is the formation of foam, which is dependent, among other factors, on yeast autolysis, aging and oenological practices.
27417558	4	51	theme	synthetic	725:733	arg1	wines					752:756	synthetic and natural base wines	725:756	synthetic and natural base wines	725:756	The use of synthetic and natural base wines allowed us to discriminate that the differences in high-molecular-weight polysaccharides and oligosaccharides could be attributed to the yeast cells and that the higher nitrogen content in the natural wine could be due to external proteolysis.
27417558	3	52	theme	yeast	590:594	arg1	addition					565:572	the addition	561:572	the addition of inactive dry yeast to the base wine	561:611	RESULTS We determined that the addition of inorganic nitrogen promoted nitrogen liberation to the extracellular medium, while the addition of inactive dry yeast to the base wine caused an increase in the polysaccharide concentration and foaming properties of the sparkling wine.
27417558	3	53	theme	inorganic	478:486	arg1	nitrogen					488:495	inorganic nitrogen	478:495	inorganic nitrogen	478:495	RESULTS We determined that the addition of inorganic nitrogen promoted nitrogen liberation to the extracellular medium, while the addition of inactive dry yeast to the base wine caused an increase in the polysaccharide concentration and foaming properties of the sparkling wine.
27417558	0	54	theme	wine	65:68	arg1	composition					70:80	sparkling wine composition	55:80	sparkling wine composition	55:80	Contribution of yeast and base wine supplementation to sparkling wine composition.
27417558	5	55	theme	nitrogen	1030:1037	arg1	addition					1039:1046	nitrogen addition	1030:1046	nitrogen addition	1030:1046	CONCLUSION The practices of nitrogen addition and supplementation of inactive dry yeast could modulate the main characteristics of the sparkling wine and be a critical element for the design of this kind of wine.
27417558	2	56	theme	supplementation	329:343	arg1	effects					295:301	the effects	291:301	the effects of yeast strain, nutrient supplementation to the base wine and aging process on the sparkling wine composition and its foamability	291:432	In this study, we analyzed the effects of yeast strain, nutrient supplementation to the base wine and aging process on the sparkling wine composition and its foamability.
27417558	5	57	dep	CONCLUSION	1002:1011	arg1	element					1170:1176	a critical element	1159:1176	a critical element for the design of this kind of wine	1159:1212	CONCLUSION The practices of nitrogen addition and supplementation of inactive dry yeast could modulate the main characteristics of the sparkling wine and be a critical element for the design of this kind of wine.
27417558	5	57	dep	CONCLUSION	1002:1011	arg1	modulate					1096:1103	modulate	1096:1103	modulate the main characteristics of the sparkling wine	1096:1150	CONCLUSION The practices of nitrogen addition and supplementation of inactive dry yeast could modulate the main characteristics of the sparkling wine and be a critical element for the design of this kind of wine.
27417558	1	58	theme	yeast	214:218	arg1	autolysis					220:228	yeast autolysis	214:228	yeast autolysis	214:228	BACKGROUND The differential characteristic of sparkling wine is the formation of foam, which is dependent, among other factors, on yeast autolysis, aging and oenological practices.
27417558	0	59	theme	sparkling	55:63	arg1	composition					70:80	sparkling wine composition	55:80	sparkling wine composition	55:80	Contribution of yeast and base wine supplementation to sparkling wine composition.
27417558	3	60	theme	inactive	577:584	arg1	yeast					590:594	inactive dry yeast	577:594	inactive dry yeast	577:594	RESULTS We determined that the addition of inorganic nitrogen promoted nitrogen liberation to the extracellular medium, while the addition of inactive dry yeast to the base wine caused an increase in the polysaccharide concentration and foaming properties of the sparkling wine.
27417558	5	61	theme	yeast	1084:1088	arg1	supplementation					1052:1066	supplementation	1052:1066	supplementation of inactive dry yeast	1052:1088	CONCLUSION The practices of nitrogen addition and supplementation of inactive dry yeast could modulate the main characteristics of the sparkling wine and be a critical element for the design of this kind of wine.
27417558	5	61	theme	yeast	1084:1088	arg1	practices					1017:1025	The practices	1013:1025	The practices of nitrogen addition	1013:1046	CONCLUSION The practices of nitrogen addition and supplementation of inactive dry yeast could modulate the main characteristics of the sparkling wine and be a critical element for the design of this kind of wine.
27417558	2	62	theme	nutrient	320:327	arg1	supplementation					329:343	nutrient supplementation	320:343	nutrient supplementation to the base wine	320:360	In this study, we analyzed the effects of yeast strain, nutrient supplementation to the base wine and aging process on the sparkling wine composition and its foamability.
27417558	5	63	theme	wine	1209:1212	arg1	kind					1201:1204	this kind	1196:1204	this kind of wine	1196:1212	CONCLUSION The practices of nitrogen addition and supplementation of inactive dry yeast could modulate the main characteristics of the sparkling wine and be a critical element for the design of this kind of wine.
25129633	6	0	theme	linear	1010:1015	arg1	RG1					1026:1028	linear arabinan RG1	1010:1028	linear arabinan RG1	1010:1028	METHODS Developing stomata were examined and immunogold-labelled in transmission electron microscopy using monoclonal antibodies to five pectin epitopes: LM19 (unesterified HG), LM20 (esterified HG), LM5 (galactan RG1), LM6 (arabinan RG1) and LM13 (linear arabinan RG1).
25129633	6	0	theme	linear	1010:1015	arg1	LM13					1004:1007	LM13	1004:1007	LM13 (linear arabinan RG1)	1004:1029	METHODS Developing stomata were examined and immunogold-labelled in transmission electron microscopy using monoclonal antibodies to five pectin epitopes: LM19 (unesterified HG), LM20 (esterified HG), LM5 (galactan RG1), LM6 (arabinan RG1) and LM13 (linear arabinan RG1).
25129633	6	1	dep	epitopes	905:912	arg1	epitopes					905:912	five pectin epitopes	893:912	five pectin epitopes: LM19 (unesterified HG), LM20 (esterified HG), LM5 (galactan RG1), LM6 (arabinan RG1) and LM13 (linear arabinan RG1)	893:1029	METHODS Developing stomata were examined and immunogold-labelled in transmission electron microscopy using monoclonal antibodies to five pectin epitopes: LM19 (unesterified HG), LM20 (esterified HG), LM5 (galactan RG1), LM6 (arabinan RG1) and LM13 (linear arabinan RG1).
25129633	6	1	dep	epitopes	905:912	arg1	LM5					961:963	LM5	961:963	LM5 (galactan RG1)	961:978	METHODS Developing stomata were examined and immunogold-labelled in transmission electron microscopy using monoclonal antibodies to five pectin epitopes: LM19 (unesterified HG), LM20 (esterified HG), LM5 (galactan RG1), LM6 (arabinan RG1) and LM13 (linear arabinan RG1).
25129633	6	1	dep	epitopes	905:912	arg1	LM6					981:983	LM6	981:983	LM6 (arabinan RG1)	981:998	METHODS Developing stomata were examined and immunogold-labelled in transmission electron microscopy using monoclonal antibodies to five pectin epitopes: LM19 (unesterified HG), LM20 (esterified HG), LM5 (galactan RG1), LM6 (arabinan RG1) and LM13 (linear arabinan RG1).
25129633	6	1	dep	epitopes	905:912	arg1	LM19					915:918	LM19	915:918	LM19 (unesterified HG)	915:936	METHODS Developing stomata were examined and immunogold-labelled in transmission electron microscopy using monoclonal antibodies to five pectin epitopes: LM19 (unesterified HG), LM20 (esterified HG), LM5 (galactan RG1), LM6 (arabinan RG1) and LM13 (linear arabinan RG1).
25129633	6	1	dep	epitopes	905:912	arg1	LM20					939:942	LM20	939:942	LM20 (esterified HG)	939:958	METHODS Developing stomata were examined and immunogold-labelled in transmission electron microscopy using monoclonal antibodies to five pectin epitopes: LM19 (unesterified HG), LM20 (esterified HG), LM5 (galactan RG1), LM6 (arabinan RG1) and LM13 (linear arabinan RG1).
25129633	6	1	dep	epitopes	905:912	arg1	RG1					1026:1028	linear arabinan RG1	1010:1028	linear arabinan RG1	1010:1028	METHODS Developing stomata were examined and immunogold-labelled in transmission electron microscopy using monoclonal antibodies to five pectin epitopes: LM19 (unesterified HG), LM20 (esterified HG), LM5 (galactan RG1), LM6 (arabinan RG1) and LM13 (linear arabinan RG1).
25129633	6	1	dep	epitopes	905:912	arg1	RG1					975:977	galactan RG1	966:977	galactan RG1	966:977	METHODS Developing stomata were examined and immunogold-labelled in transmission electron microscopy using monoclonal antibodies to five pectin epitopes: LM19 (unesterified HG), LM20 (esterified HG), LM5 (galactan RG1), LM6 (arabinan RG1) and LM13 (linear arabinan RG1).
25129633	6	1	dep	epitopes	905:912	arg1	RG1					995:997	arabinan RG1	986:997	arabinan RG1	986:997	METHODS Developing stomata were examined and immunogold-labelled in transmission electron microscopy using monoclonal antibodies to five pectin epitopes: LM19 (unesterified HG), LM20 (esterified HG), LM5 (galactan RG1), LM6 (arabinan RG1) and LM13 (linear arabinan RG1).
25129633	6	1	dep	epitopes	905:912	arg1	HG					934:935	unesterified HG	921:935	unesterified HG	921:935	METHODS Developing stomata were examined and immunogold-labelled in transmission electron microscopy using monoclonal antibodies to five pectin epitopes: LM19 (unesterified HG), LM20 (esterified HG), LM5 (galactan RG1), LM6 (arabinan RG1) and LM13 (linear arabinan RG1).
25129633	6	1	dep	epitopes	905:912	arg1	HG					956:957	esterified HG	945:957	esterified HG	945:957	METHODS Developing stomata were examined and immunogold-labelled in transmission electron microscopy using monoclonal antibodies to five pectin epitopes: LM19 (unesterified HG), LM20 (esterified HG), LM5 (galactan RG1), LM6 (arabinan RG1) and LM13 (linear arabinan RG1).
25129633	6	1	dep	epitopes	905:912	arg1	LM13					1004:1007	LM13	1004:1007	LM13 (linear arabinan RG1)	1004:1029	METHODS Developing stomata were examined and immunogold-labelled in transmission electron microscopy using monoclonal antibodies to five pectin epitopes: LM19 (unesterified HG), LM20 (esterified HG), LM5 (galactan RG1), LM6 (arabinan RG1) and LM13 (linear arabinan RG1).
25129633	3	2	theme	cell	466:469	arg1	function					471:478	guard cell function	460:478	guard cell function	460:478	Recognized as the first extant plants with stomata, mosses are key to understanding guard cell function and evolution.
25129633	16	3	from	Changes	1956:1962	arg1	architecture					1972:1983	wall architecture	1967:1983	wall architecture	1967:1983	Changes in wall architecture coupled with a decrease in total pectin may be responsible for the inability of mature stomata to move.
25129633	6	4	theme	pectin	898:903	arg1	epitopes					905:912	five pectin epitopes	893:912	five pectin epitopes: LM19 (unesterified HG), LM20 (esterified HG), LM5 (galactan RG1), LM6 (arabinan RG1) and LM13 (linear arabinan RG1)	893:1029	METHODS Developing stomata were examined and immunogold-labelled in transmission electron microscopy using monoclonal antibodies to five pectin epitopes: LM19 (unesterified HG), LM20 (esterified HG), LM5 (galactan RG1), LM6 (arabinan RG1) and LM13 (linear arabinan RG1).
25129633	6	4	theme	pectin	898:903	arg1	LM5					961:963	LM5	961:963	LM5 (galactan RG1)	961:978	METHODS Developing stomata were examined and immunogold-labelled in transmission electron microscopy using monoclonal antibodies to five pectin epitopes: LM19 (unesterified HG), LM20 (esterified HG), LM5 (galactan RG1), LM6 (arabinan RG1) and LM13 (linear arabinan RG1).
25129633	6	4	theme	pectin	898:903	arg1	LM6					981:983	LM6	981:983	LM6 (arabinan RG1)	981:998	METHODS Developing stomata were examined and immunogold-labelled in transmission electron microscopy using monoclonal antibodies to five pectin epitopes: LM19 (unesterified HG), LM20 (esterified HG), LM5 (galactan RG1), LM6 (arabinan RG1) and LM13 (linear arabinan RG1).
25129633	6	4	theme	pectin	898:903	arg1	LM19					915:918	LM19	915:918	LM19 (unesterified HG)	915:936	METHODS Developing stomata were examined and immunogold-labelled in transmission electron microscopy using monoclonal antibodies to five pectin epitopes: LM19 (unesterified HG), LM20 (esterified HG), LM5 (galactan RG1), LM6 (arabinan RG1) and LM13 (linear arabinan RG1).
25129633	6	4	theme	pectin	898:903	arg1	LM20					939:942	LM20	939:942	LM20 (esterified HG)	939:958	METHODS Developing stomata were examined and immunogold-labelled in transmission electron microscopy using monoclonal antibodies to five pectin epitopes: LM19 (unesterified HG), LM20 (esterified HG), LM5 (galactan RG1), LM6 (arabinan RG1) and LM13 (linear arabinan RG1).
25129633	6	4	theme	pectin	898:903	arg1	LM13					1004:1007	LM13	1004:1007	LM13 (linear arabinan RG1)	1004:1029	METHODS Developing stomata were examined and immunogold-labelled in transmission electron microscopy using monoclonal antibodies to five pectin epitopes: LM19 (unesterified HG), LM20 (esterified HG), LM5 (galactan RG1), LM6 (arabinan RG1) and LM13 (linear arabinan RG1).
25129633	3	5	theme	guard	460:464	arg1	function					471:478	guard cell function	460:478	guard cell function	460:478	Recognized as the first extant plants with stomata, mosses are key to understanding guard cell function and evolution.
25129633	5	6	from	development	662:672	arg1	hygrometrica					685:696	Funaria hygrometrica	677:696	Funaria hygrometrica	677:696	This study examines the ultrastructure and pectin composition of guard cell walls during development in Funaria hygrometrica and relates these features to the limited movement of stomata.
25129633	8	7	theme	RESULTS	1149:1155	arg1	Walls					1157:1161	KEY RESULTS Walls	1145:1161	KEY RESULTS Walls	1145:1161	KEY RESULTS Walls were four times thinner before pore formation than in mature stomata.
25129633	13	8	theme	HG	1684:1685	arg1	proportion					1670:1679	the proportion	1666:1679	the proportion of HG to arabinans	1666:1698	Pectin content decreased but the proportion of HG to arabinans changed only slightly.
25129633	9	9	theme	thickest	1342:1349	arg1	layer					1351:1355	the striated internal and thickest layer	1316:1355	layer	1351:1355	When stomata opened and closed, guard cell walls were thin and pectinaceous before the striated internal and thickest layer was deposited.
25129633	6	10	theme	monoclonal	868:877	arg1	antibodies					879:888	monoclonal antibodies	868:888	monoclonal antibodies to five pectin epitopes: LM19 (unesterified HG), LM20 (esterified HG), LM5 (galactan RG1), LM6 (arabinan RG1) and LM13 (linear arabinan RG1)	868:1029	METHODS Developing stomata were examined and immunogold-labelled in transmission electron microscopy using monoclonal antibodies to five pectin epitopes: LM19 (unesterified HG), LM20 (esterified HG), LM5 (galactan RG1), LM6 (arabinan RG1) and LM13 (linear arabinan RG1).
25129633	12	11	theme	guard	1619:1623	arg1	walls					1630:1634	guard cell walls	1619:1634	guard cell walls	1619:1634	Linear arabinan RG1 is the only pectin that exclusively labelled guard cell walls.
25129633	1	12	theme	seed	170:173	arg1	plants					175:180	seed plants	170:180	seed plants	170:180	BACKGROUND AND AIMS In seed plants, the ability of guard cell walls to move is imparted by pectins.
25129633	2	13	theme	rhamnogalacturonan	256:273	arg1	pectins					283:289	Arabinan rhamnogalacturonan I (RG1) pectins	247:289	Arabinan rhamnogalacturonan I (RG1) pectins	247:289	Arabinan rhamnogalacturonan I (RG1) pectins confer flexibility while unesterified homogalacturonan (HG) pectins impart rigidity.
25129633	16	14	from	decrease	2000:2007	arg1	pectin					2018:2023	total pectin	2012:2023	total pectin	2012:2023	Changes in wall architecture coupled with a decrease in total pectin may be responsible for the inability of mature stomata to move.
25129633	5	15	theme	guard	638:642	arg1	walls					649:653	guard cell walls	638:653	guard cell walls	638:653	This study examines the ultrastructure and pectin composition of guard cell walls during development in Funaria hygrometrica and relates these features to the limited movement of stomata.
25129633	14	16	theme	first	1747:1751	arg1	This					1735:1738	This	1735:1738	This	1735:1738	CONCLUSIONS This is the first study to demonstrate changes in pectin composition during stomatal development in any plant.
25129633	14	16	theme	first	1747:1751	arg1	study					1753:1757	the first study	1743:1757	the first study to demonstrate changes in pectin composition during stomatal development in any plant	1743:1843	CONCLUSIONS This is the first study to demonstrate changes in pectin composition during stomatal development in any plant.
25129633	5	17	theme	walls	649:653	arg1	ultrastructure					597:610	ultrastructure	597:610	ultrastructure	597:610	This study examines the ultrastructure and pectin composition of guard cell walls during development in Funaria hygrometrica and relates these features to the limited movement of stomata.
25129633	5	17	theme	walls	649:653	arg1	composition					623:633	pectin composition	616:633	pectin composition	616:633	This study examines the ultrastructure and pectin composition of guard cell walls during development in Funaria hygrometrica and relates these features to the limited movement of stomata.
25129633	17	18	from	Specialization	2089:2102	arg1	mosses					2122:2127	mosses	2122:2127	mosses	2122:2127	Specialization of guard cells in mosses involves the addition of linear arabinans.
25129633	0	19	theme	pectin	55:60	arg1	composition					62:72	pectin composition	55:72	pectin composition	55:72	Developmental changes in guard cell wall structure and pectin composition in the moss Funaria: implications for function and evolution of stomata.
25129633	3	20	with	plants	407:412	arg1	stomata					419:425	stomata	419:425	stomata	419:425	Recognized as the first extant plants with stomata, mosses are key to understanding guard cell function and evolution.
25129633	5	21	theme	limited	732:738	arg1	movement					740:747	the limited movement	728:747	the limited movement of stomata	728:758	This study examines the ultrastructure and pectin composition of guard cell walls during development in Funaria hygrometrica and relates these features to the limited movement of stomata.
25129633	12	22	theme	only	1581:1584	arg1	RG1					1570:1572	Linear arabinan RG1	1554:1572	Linear arabinan RG1	1554:1572	Linear arabinan RG1 is the only pectin that exclusively labelled guard cell walls.
25129633	12	22	theme	only	1581:1584	arg1	pectin					1586:1591	the only pectin	1577:1591	the only pectin that exclusively labelled guard cell walls	1577:1634	Linear arabinan RG1 is the only pectin that exclusively labelled guard cell walls.
25129633	17	23	theme	guard	2107:2111	arg1	cells					2113:2117	guard cells	2107:2117	guard cells	2107:2117	Specialization of guard cells in mosses involves the addition of linear arabinans.
25129633	0	24	theme	moss	81:84	arg1	Funaria					86:92	the moss Funaria	77:92	the moss Funaria	77:92	Developmental changes in guard cell wall structure and pectin composition in the moss Funaria: implications for function and evolution of stomata.
25129633	3	25	theme	extant	400:405	arg1	plants					407:412	the first extant plants	390:412	the first extant plants with stomata	390:425	Recognized as the first extant plants with stomata, mosses are key to understanding guard cell function and evolution.
25129633	12	26	theme	arabinan	1561:1568	arg1	RG1					1570:1572	Linear arabinan RG1	1554:1572	Linear arabinan RG1	1554:1572	Linear arabinan RG1 is the only pectin that exclusively labelled guard cell walls.
25129633	12	26	theme	arabinan	1561:1568	arg1	pectin					1586:1591	the only pectin	1577:1591	the only pectin that exclusively labelled guard cell walls	1577:1634	Linear arabinan RG1 is the only pectin that exclusively labelled guard cell walls.
25129633	17	27	theme	linear	2154:2159	arg1	arabinans					2161:2169	linear arabinans	2154:2169	linear arabinans	2154:2169	Specialization of guard cells in mosses involves the addition of linear arabinans.
25129633	16	28	theme	total	2012:2016	arg1	pectin					2018:2023	total pectin	2012:2023	total pectin	2012:2023	Changes in wall architecture coupled with a decrease in total pectin may be responsible for the inability of mature stomata to move.
25129633	0	29	theme	Developmental	0:12	arg1	changes					14:20	Developmental changes	0:20	Developmental changes in guard cell wall structure and pectin composition in the moss Funaria: implications for function and evolution of stomata.	0:145	Developmental changes in guard cell wall structure and pectin composition in the moss Funaria: implications for function and evolution of stomata.
25129633	5	30	dep	ultrastructure	597:610	arg1	the					593:595	the	593:595	the	593:595	This study examines the ultrastructure and pectin composition of guard cell walls during development in Funaria hygrometrica and relates these features to the limited movement of stomata.
25129633	10	31	theme	early	1410:1414	arg1	layers					1416:1421	early layers	1410:1421	early layers	1410:1421	Unesterified HG localized strongly in early layers but weakly in the thick internal layer.
25129633	15	32	theme	cell	1932:1935	arg1	walls					1937:1941	guard cell walls	1926:1941	guard cell walls	1926:1941	Movement of Funaria stomata coincides with capsule expansion before layering of guard cell walls is complete.
25129633	0	33	theme	cell	31:34	arg1	structure					41:49	guard cell wall structure	25:49	guard cell wall structure	25:49	Developmental changes in guard cell wall structure and pectin composition in the moss Funaria: implications for function and evolution of stomata.
25129633	6	34	theme	electron	842:849	arg1	microscopy					851:860	transmission electron microscopy	829:860	transmission electron microscopy using monoclonal antibodies to five pectin epitopes: LM19 (unesterified HG), LM20 (esterified HG), LM5 (galactan RG1), LM6 (arabinan RG1) and LM13 (linear arabinan RG1)	829:1029	METHODS Developing stomata were examined and immunogold-labelled in transmission electron microscopy using monoclonal antibodies to five pectin epitopes: LM19 (unesterified HG), LM20 (esterified HG), LM5 (galactan RG1), LM6 (arabinan RG1) and LM13 (linear arabinan RG1).
25129633	9	35	theme	cell	1271:1274	arg1	walls					1276:1280	guard cell walls	1265:1280	guard cell walls	1265:1280	When stomata opened and closed, guard cell walls were thin and pectinaceous before the striated internal and thickest layer was deposited.
25129633	4	36	theme	Moss	495:498	arg1	stomata					500:506	Moss stomata	495:506	Moss stomata	495:506	Moss stomata open and close for only a short period during capsule expansion.
25129633	15	37	theme	stomata	1866:1872	arg1	Movement					1846:1853	Movement	1846:1853	Movement of Funaria stomata	1846:1872	Movement of Funaria stomata coincides with capsule expansion before layering of guard cell walls is complete.
25129633	11	38	theme	esterified	1486:1495	arg1	HG					1497:1498	esterified HG	1486:1498	esterified HG	1486:1498	Labelling was weak for esterified HG, absent for galactan RG1 and strong for arabinan RG1.
25129633	16	39	theme	stomata	2072:2078	arg1	inability					2052:2060	the inability	2048:2060	the inability of mature stomata to move	2048:2086	Changes in wall architecture coupled with a decrease in total pectin may be responsible for the inability of mature stomata to move.
25129633	1	40	theme	cell	204:207	arg1	walls					209:213	guard cell walls	198:213	guard cell walls	198:213	BACKGROUND AND AIMS In seed plants, the ability of guard cell walls to move is imparted by pectins.
25129633	5	41	theme	stomata	752:758	arg1	movement					740:747	the limited movement	728:747	the limited movement of stomata	728:758	This study examines the ultrastructure and pectin composition of guard cell walls during development in Funaria hygrometrica and relates these features to the limited movement of stomata.
25129633	6	42	theme	arabinan	1017:1024	arg1	RG1					1026:1028	linear arabinan RG1	1010:1028	linear arabinan RG1	1010:1028	METHODS Developing stomata were examined and immunogold-labelled in transmission electron microscopy using monoclonal antibodies to five pectin epitopes: LM19 (unesterified HG), LM20 (esterified HG), LM5 (galactan RG1), LM6 (arabinan RG1) and LM13 (linear arabinan RG1).
25129633	6	42	theme	arabinan	1017:1024	arg1	LM13					1004:1007	LM13	1004:1007	LM13 (linear arabinan RG1)	1004:1029	METHODS Developing stomata were examined and immunogold-labelled in transmission electron microscopy using monoclonal antibodies to five pectin epitopes: LM19 (unesterified HG), LM20 (esterified HG), LM5 (galactan RG1), LM6 (arabinan RG1) and LM13 (linear arabinan RG1).
25129633	10	43	theme	thick	1441:1445	arg1	layer					1456:1460	the thick internal layer	1437:1460	the thick internal layer	1437:1460	Unesterified HG localized strongly in early layers but weakly in the thick internal layer.
25129633	11	44	theme	arabinan	1540:1547	arg1	RG1					1549:1551	arabinan RG1	1540:1551	arabinan RG1	1540:1551	Labelling was weak for esterified HG, absent for galactan RG1 and strong for arabinan RG1.
25129633	2	45	theme	unesterified	316:327	arg1	HG					347:348	HG	347:348	HG	347:348	Arabinan rhamnogalacturonan I (RG1) pectins confer flexibility while unesterified homogalacturonan (HG) pectins impart rigidity.
25129633	2	45	theme	unesterified	316:327	arg1	homogalacturonan					329:344	unesterified homogalacturonan	316:344	unesterified homogalacturonan (HG) pectins	316:357	Arabinan rhamnogalacturonan I (RG1) pectins confer flexibility while unesterified homogalacturonan (HG) pectins impart rigidity.
25129633	5	46	theme	pectin	616:621	arg1	composition					623:633	pectin composition	616:633	pectin composition	616:633	This study examines the ultrastructure and pectin composition of guard cell walls during development in Funaria hygrometrica and relates these features to the limited movement of stomata.
25129633	2	47	theme	Arabinan	247:254	arg1	pectins					283:289	Arabinan rhamnogalacturonan I (RG1) pectins	247:289	Arabinan rhamnogalacturonan I (RG1) pectins	247:289	Arabinan rhamnogalacturonan I (RG1) pectins confer flexibility while unesterified homogalacturonan (HG) pectins impart rigidity.
25129633	4	48	theme	short	534:538	arg1	period					540:545	only a short period	527:545	only a short period during capsule expansion	527:570	Moss stomata open and close for only a short period during capsule expansion.
25129633	14	49	theme	stomatal	1811:1818	arg1	development					1820:1830	stomatal development	1811:1830	stomatal development in any plant	1811:1843	CONCLUSIONS This is the first study to demonstrate changes in pectin composition during stomatal development in any plant.
25129633	12	50	theme	cell	1625:1628	arg1	walls					1630:1634	guard cell walls	1619:1634	guard cell walls	1619:1634	Linear arabinan RG1 is the only pectin that exclusively labelled guard cell walls.
25129633	8	51	theme	KEY	1145:1147	arg1	Walls					1157:1161	KEY RESULTS Walls	1145:1161	KEY RESULTS Walls	1145:1161	KEY RESULTS Walls were four times thinner before pore formation than in mature stomata.
25129633	13	52	theme	Pectin	1637:1642	arg1	content					1644:1650	Pectin content	1637:1650	Pectin content	1637:1650	Pectin content decreased but the proportion of HG to arabinans changed only slightly.
25129633	2	53	theme	RG1	278:280	arg1	pectins					283:289	Arabinan rhamnogalacturonan I (RG1) pectins	247:289	Arabinan rhamnogalacturonan I (RG1) pectins	247:289	Arabinan rhamnogalacturonan I (RG1) pectins confer flexibility while unesterified homogalacturonan (HG) pectins impart rigidity.
25129633	6	54	theme	esterified	945:954	arg1	LM20					939:942	LM20	939:942	LM20 (esterified HG)	939:958	METHODS Developing stomata were examined and immunogold-labelled in transmission electron microscopy using monoclonal antibodies to five pectin epitopes: LM19 (unesterified HG), LM20 (esterified HG), LM5 (galactan RG1), LM6 (arabinan RG1) and LM13 (linear arabinan RG1).
25129633	6	54	theme	esterified	945:954	arg1	HG					956:957	esterified HG	945:957	esterified HG	945:957	METHODS Developing stomata were examined and immunogold-labelled in transmission electron microscopy using monoclonal antibodies to five pectin epitopes: LM19 (unesterified HG), LM20 (esterified HG), LM5 (galactan RG1), LM6 (arabinan RG1) and LM13 (linear arabinan RG1).
25129633	16	55	theme	mature	2065:2070	arg1	stomata					2072:2078	mature stomata	2065:2078	mature stomata	2065:2078	Changes in wall architecture coupled with a decrease in total pectin may be responsible for the inability of mature stomata to move.
25129633	16	56	theme	wall	1967:1970	arg1	architecture					1972:1983	wall architecture	1967:1983	wall architecture	1967:1983	Changes in wall architecture coupled with a decrease in total pectin may be responsible for the inability of mature stomata to move.
25129633	2	57	theme	I	275:275	arg1	pectins					283:289	Arabinan rhamnogalacturonan I (RG1) pectins	247:289	Arabinan rhamnogalacturonan I (RG1) pectins	247:289	Arabinan rhamnogalacturonan I (RG1) pectins confer flexibility while unesterified homogalacturonan (HG) pectins impart rigidity.
25129633	17	58	theme	arabinans	2161:2169	arg1	addition					2142:2149	the addition	2138:2149	the addition of linear arabinans	2138:2169	Specialization of guard cells in mosses involves the addition of linear arabinans.
25129633	14	59	theme	pectin	1785:1790	arg1	composition					1792:1802	pectin composition	1785:1802	pectin composition	1785:1802	CONCLUSIONS This is the first study to demonstrate changes in pectin composition during stomatal development in any plant.
25129633	8	60	theme	thinner	1179:1185	arg1	times					1173:1177	four times	1168:1177	four times thinner before pore formation than in mature stomata	1168:1230	KEY RESULTS Walls were four times thinner before pore formation than in mature stomata.
25129633	0	61	theme	stomata	138:144	arg1	evolution					125:133	evolution	125:133	evolution	125:133	Developmental changes in guard cell wall structure and pectin composition in the moss Funaria: implications for function and evolution of stomata.
25129633	0	61	theme	stomata	138:144	arg1	function					112:119	function	112:119	function	112:119	Developmental changes in guard cell wall structure and pectin composition in the moss Funaria: implications for function and evolution of stomata.
25129633	6	62	theme	unesterified	921:932	arg1	HG					934:935	unesterified HG	921:935	unesterified HG	921:935	METHODS Developing stomata were examined and immunogold-labelled in transmission electron microscopy using monoclonal antibodies to five pectin epitopes: LM19 (unesterified HG), LM20 (esterified HG), LM5 (galactan RG1), LM6 (arabinan RG1) and LM13 (linear arabinan RG1).
25129633	6	62	theme	unesterified	921:932	arg1	LM19					915:918	LM19	915:918	LM19 (unesterified HG)	915:936	METHODS Developing stomata were examined and immunogold-labelled in transmission electron microscopy using monoclonal antibodies to five pectin epitopes: LM19 (unesterified HG), LM20 (esterified HG), LM5 (galactan RG1), LM6 (arabinan RG1) and LM13 (linear arabinan RG1).
25129633	8	63	theme	pore	1194:1197	arg1	formation					1199:1207	pore formation	1194:1207	pore formation	1194:1207	KEY RESULTS Walls were four times thinner before pore formation than in mature stomata.
25129633	5	64	theme	cell	644:647	arg1	walls					649:653	guard cell walls	638:653	guard cell walls	638:653	This study examines the ultrastructure and pectin composition of guard cell walls during development in Funaria hygrometrica and relates these features to the limited movement of stomata.
25129633	14	65	from	development	1820:1830	arg1	plant					1839:1843	any plant	1835:1843	any plant	1835:1843	CONCLUSIONS This is the first study to demonstrate changes in pectin composition during stomatal development in any plant.
25129633	6	66	theme	galactan	966:973	arg1	LM5					961:963	LM5	961:963	LM5 (galactan RG1)	961:978	METHODS Developing stomata were examined and immunogold-labelled in transmission electron microscopy using monoclonal antibodies to five pectin epitopes: LM19 (unesterified HG), LM20 (esterified HG), LM5 (galactan RG1), LM6 (arabinan RG1) and LM13 (linear arabinan RG1).
25129633	6	66	theme	galactan	966:973	arg1	RG1					975:977	galactan RG1	966:977	galactan RG1	966:977	METHODS Developing stomata were examined and immunogold-labelled in transmission electron microscopy using monoclonal antibodies to five pectin epitopes: LM19 (unesterified HG), LM20 (esterified HG), LM5 (galactan RG1), LM6 (arabinan RG1) and LM13 (linear arabinan RG1).
25129633	17	67	theme	cells	2113:2117	arg1	Specialization					2089:2102	Specialization	2089:2102	Specialization of guard cells in mosses	2089:2127	Specialization of guard cells in mosses involves the addition of linear arabinans.
25129633	8	68	theme	mature	1217:1222	arg1	stomata					1224:1230	mature stomata	1217:1230	mature stomata	1217:1230	KEY RESULTS Walls were four times thinner before pore formation than in mature stomata.
25129633	7	69	theme	replicated	1112:1121	arg1	samples					1136:1142	replicated, independent samples	1112:1142	replicated, independent samples	1112:1142	Labels for pectin type were quantitated and compared across walls and stages on replicated, independent samples.
25129633	0	70	from	changes	14:20	arg1	Funaria					86:92	the moss Funaria	77:92	the moss Funaria	77:92	Developmental changes in guard cell wall structure and pectin composition in the moss Funaria: implications for function and evolution of stomata.
25129633	0	70	from	changes	14:20	arg1	structure					41:49	guard cell wall structure	25:49	guard cell wall structure	25:49	Developmental changes in guard cell wall structure and pectin composition in the moss Funaria: implications for function and evolution of stomata.
25129633	0	70	from	changes	14:20	arg1	composition					62:72	pectin composition	55:72	pectin composition	55:72	Developmental changes in guard cell wall structure and pectin composition in the moss Funaria: implications for function and evolution of stomata.
25129633	15	71	theme	Funaria	1858:1864	arg1	stomata					1866:1872	Funaria stomata	1858:1872	Funaria stomata	1858:1872	Movement of Funaria stomata coincides with capsule expansion before layering of guard cell walls is complete.
25129633	10	72	theme	Unesterified	1372:1383	arg1	HG					1385:1386	Unesterified HG	1372:1386	Unesterified HG	1372:1386	Unesterified HG localized strongly in early layers but weakly in the thick internal layer.
25129633	15	73	theme	walls	1937:1941	arg1	layering					1914:1921	layering	1914:1921	layering of guard cell walls	1914:1941	Movement of Funaria stomata coincides with capsule expansion before layering of guard cell walls is complete.
25129633	12	74	theme	Linear	1554:1559	arg1	RG1					1570:1572	Linear arabinan RG1	1554:1572	Linear arabinan RG1	1554:1572	Linear arabinan RG1 is the only pectin that exclusively labelled guard cell walls.
25129633	12	74	theme	Linear	1554:1559	arg1	pectin					1586:1591	the only pectin	1577:1591	the only pectin that exclusively labelled guard cell walls	1577:1634	Linear arabinan RG1 is the only pectin that exclusively labelled guard cell walls.
25129633	1	75	dep	BACKGROUND	147:156	arg1	imparted					226:233	imparted	226:233	is imparted by pectins	223:244	BACKGROUND AND AIMS In seed plants, the ability of guard cell walls to move is imparted by pectins.
25129633	3	76	theme	first	394:398	arg1	plants					407:412	the first extant plants	390:412	the first extant plants with stomata	390:425	Recognized as the first extant plants with stomata, mosses are key to understanding guard cell function and evolution.
25129633	0	77	theme	guard	25:29	arg1	structure					41:49	guard cell wall structure	25:49	guard cell wall structure	25:49	Developmental changes in guard cell wall structure and pectin composition in the moss Funaria: implications for function and evolution of stomata.
25129633	15	78	theme	guard	1926:1930	arg1	walls					1937:1941	guard cell walls	1926:1941	guard cell walls	1926:1941	Movement of Funaria stomata coincides with capsule expansion before layering of guard cell walls is complete.
25129633	6	79	theme	arabinan	986:993	arg1	LM6					981:983	LM6	981:983	LM6 (arabinan RG1)	981:998	METHODS Developing stomata were examined and immunogold-labelled in transmission electron microscopy using monoclonal antibodies to five pectin epitopes: LM19 (unesterified HG), LM20 (esterified HG), LM5 (galactan RG1), LM6 (arabinan RG1) and LM13 (linear arabinan RG1).
25129633	6	79	theme	arabinan	986:993	arg1	RG1					995:997	arabinan RG1	986:997	arabinan RG1	986:997	METHODS Developing stomata were examined and immunogold-labelled in transmission electron microscopy using monoclonal antibodies to five pectin epitopes: LM19 (unesterified HG), LM20 (esterified HG), LM5 (galactan RG1), LM6 (arabinan RG1) and LM13 (linear arabinan RG1).
25129633	14	80	from	changes	1774:1780	arg1	composition					1792:1802	pectin composition	1785:1802	pectin composition	1785:1802	CONCLUSIONS This is the first study to demonstrate changes in pectin composition during stomatal development in any plant.
25129633	0	81	theme	wall	36:39	arg1	structure					41:49	guard cell wall structure	25:49	guard cell wall structure	25:49	Developmental changes in guard cell wall structure and pectin composition in the moss Funaria: implications for function and evolution of stomata.
25129633	7	82	theme	pectin	1043:1048	arg1	type					1050:1053	pectin type	1043:1053	pectin type	1043:1053	Labels for pectin type were quantitated and compared across walls and stages on replicated, independent samples.
25129633	9	83	theme	guard	1265:1269	arg1	walls					1276:1280	guard cell walls	1265:1280	guard cell walls	1265:1280	When stomata opened and closed, guard cell walls were thin and pectinaceous before the striated internal and thickest layer was deposited.
25129633	6	84	theme	transmission	829:840	arg1	microscopy					851:860	transmission electron microscopy	829:860	transmission electron microscopy using monoclonal antibodies to five pectin epitopes: LM19 (unesterified HG), LM20 (esterified HG), LM5 (galactan RG1), LM6 (arabinan RG1) and LM13 (linear arabinan RG1)	829:1029	METHODS Developing stomata were examined and immunogold-labelled in transmission electron microscopy using monoclonal antibodies to five pectin epitopes: LM19 (unesterified HG), LM20 (esterified HG), LM5 (galactan RG1), LM6 (arabinan RG1) and LM13 (linear arabinan RG1).
25129633	15	85	theme	capsule	1889:1895	arg1	expansion					1897:1905	capsule expansion	1889:1905	capsule expansion	1889:1905	Movement of Funaria stomata coincides with capsule expansion before layering of guard cell walls is complete.
25129633	4	86	theme	capsule	554:560	arg1	expansion					562:570	capsule expansion	554:570	capsule expansion	554:570	Moss stomata open and close for only a short period during capsule expansion.
25129633	7	87	dep	replicated	1112:1121	arg1	independent					1124:1134	independent	1124:1134	independent	1124:1134	Labels for pectin type were quantitated and compared across walls and stages on replicated, independent samples.
25129633	1	88	dep	ability	187:193	arg1	move					218:221	move	218:221	to move	215:221	BACKGROUND AND AIMS In seed plants, the ability of guard cell walls to move is imparted by pectins.
25129633	11	89	theme	galactan	1512:1519	arg1	RG1					1521:1523	galactan RG1	1512:1523	galactan RG1	1512:1523	Labelling was weak for esterified HG, absent for galactan RG1 and strong for arabinan RG1.
25129633	1	90	theme	guard	198:202	arg1	walls					209:213	guard cell walls	198:213	guard cell walls	198:213	BACKGROUND AND AIMS In seed plants, the ability of guard cell walls to move is imparted by pectins.
25129633	0	91	dep	changes	14:20	arg1	implications					95:106	implications	95:106	Developmental changes in guard cell wall structure and pectin composition in the moss Funaria: implications for function and evolution of stomata.	0:145	Developmental changes in guard cell wall structure and pectin composition in the moss Funaria: implications for function and evolution of stomata.
25129633	10	92	theme	internal	1447:1454	arg1	layer					1456:1460	the thick internal layer	1437:1460	the thick internal layer	1437:1460	Unesterified HG localized strongly in early layers but weakly in the thick internal layer.
25129633	2	93	theme	homogalacturonan	329:344	arg1	pectins					351:357	unesterified homogalacturonan (HG) pectins	316:357	unesterified homogalacturonan (HG) pectins	316:357	Arabinan rhamnogalacturonan I (RG1) pectins confer flexibility while unesterified homogalacturonan (HG) pectins impart rigidity.
25129633	1	94	theme	walls	209:213	arg1	ability					187:193	the ability	183:193	the ability of guard cell walls to move	183:221	BACKGROUND AND AIMS In seed plants, the ability of guard cell walls to move is imparted by pectins.
24399509	9	0	theme	composites	1148:1157	arg1	potential					1121:1129	the potential	1117:1129	the potential of the CPC-CS/HA composites to be used in bone tissue engineering	1117:1195	These results reveal the potential of the CPC-CS/HA composites to be used in bone tissue engineering.
24399509	6	1	dep	viability	731:739	arg1	The					727:729	The	727:729	The	727:729	The viability and growth rate of the cells were similar for all CPCs, but better alkaline phosphatase activity was observed for CPC with CS and HA.
24399509	5	2	dep	phosphate	701:709	arg1	cements					711:717	cements	711:717	cements	711:717	The in vitro osteoblastic cell biocompatibility of in situ forming cements was evaluated and compared with those of conventional calcium phosphate cements (CPCs).
24399509	5	3	theme	osteoblastic	577:588	arg1	biocompatibility					595:610	The in vitro osteoblastic cell biocompatibility	564:610	The in vitro osteoblastic cell biocompatibility of in situ forming cements	564:637	The in vitro osteoblastic cell biocompatibility of in situ forming cements was evaluated and compared with those of conventional calcium phosphate cements (CPCs).
24399509	6	4	theme	phosphatase	817:827	arg1	activity					829:836	better alkaline phosphatase activity	801:836	better alkaline phosphatase activity	801:836	The viability and growth rate of the cells were similar for all CPCs, but better alkaline phosphatase activity was observed for CPC with CS and HA.
24399509	3	5	theme	cements	328:334	arg1	composition					284:294	composition	284:294	composition	284:294	The composition and morphological properties of cements were evaluated by X-ray diffraction and scanning electron microscopy.
24399509	3	5	theme	cements	328:334	arg1	properties					314:323	morphological properties	300:323	morphological properties	300:323	The composition and morphological properties of cements were evaluated by X-ray diffraction and scanning electron microscopy.
24399509	9	6	theme	tissue	1178:1183	arg1	engineering					1185:1195	bone tissue engineering	1173:1195	bone tissue engineering	1173:1195	These results reveal the potential of the CPC-CS/HA composites to be used in bone tissue engineering.
24399509	5	7	theme	cell	590:593	arg1	biocompatibility					595:610	The in vitro osteoblastic cell biocompatibility	564:610	The in vitro osteoblastic cell biocompatibility of in situ forming cements	564:637	The in vitro osteoblastic cell biocompatibility of in situ forming cements was evaluated and compared with those of conventional calcium phosphate cements (CPCs).
24399509	4	8	theme	apatite	465:471	arg1	morphologies					473:484	different apatite morphologies	455:484	different apatite morphologies	455:484	The acellular in vitro bioactivity revealed that different apatite morphologies were formed on the surfaces of cements after soaking in simulated body fluid.
24399509	8	9	theme	Spindle-shaped	963:976	arg1	osteoblasts					978:988	Spindle-shaped osteoblasts	963:988	Spindle-shaped osteoblasts with developed cytoplasmic membrane	963:1024	Spindle-shaped osteoblasts with developed cytoplasmic membrane were found on the surfaces of cement samples after 7 days of culture.
24399509	4	10	theme	different	455:463	arg1	morphologies					473:484	different apatite morphologies	455:484	different apatite morphologies	455:484	The acellular in vitro bioactivity revealed that different apatite morphologies were formed on the surfaces of cements after soaking in simulated body fluid.
24399509	5	11	theme	in	615:616	arg1	biocompatibility					595:610	The in vitro osteoblastic cell biocompatibility	564:610	The in vitro osteoblastic cell biocompatibility of in situ forming cements	564:637	The in vitro osteoblastic cell biocompatibility of in situ forming cements was evaluated and compared with those of conventional calcium phosphate cements (CPCs).
24399509	7	12	theme	osteoblastic	925:936	arg1	cells					938:942	osteoblastic cells	925:942	osteoblastic cells	925:942	Calcium phosphate cements supported attachment of osteoblastic cells on their surfaces.
24399509	8	13	located	found	1031:1035	arg1	surfaces					1044:1051	the surfaces	1040:1051	the surfaces of cement samples	1040:1069	Spindle-shaped osteoblasts with developed cytoplasmic membrane were found on the surfaces of cement samples after 7 days of culture.
24399509	8	13	located	found	1031:1035	arg2	osteoblasts					978:988	Spindle-shaped osteoblasts	963:988	Spindle-shaped osteoblasts with developed cytoplasmic membrane	963:1024	Spindle-shaped osteoblasts with developed cytoplasmic membrane were found on the surfaces of cement samples after 7 days of culture.
24399509	0	14	theme	calcium	65:71	arg1	bone					83:86	chitosan/hyaluronic acid-containing calcium phosphate bone	29:86	chitosan/hyaluronic acid-containing calcium phosphate bone	29:86	In vitro biocompatibility of chitosan/hyaluronic acid-containing calcium phosphate bone cements.
24399509	0	15	theme	acid-containing	49:63	arg1	bone					83:86	chitosan/hyaluronic acid-containing calcium phosphate bone	29:86	chitosan/hyaluronic acid-containing calcium phosphate bone	29:86	In vitro biocompatibility of chitosan/hyaluronic acid-containing calcium phosphate bone cements.
24399509	9	16	theme	bone	1173:1176	arg1	engineering					1185:1195	bone tissue engineering	1173:1195	bone tissue engineering	1173:1195	These results reveal the potential of the CPC-CS/HA composites to be used in bone tissue engineering.
24399509	3	17	theme	X-ray	354:358	arg1	diffraction					360:370	X-ray diffraction	354:370	X-ray diffraction	354:370	The composition and morphological properties of cements were evaluated by X-ray diffraction and scanning electron microscopy.
24399509	8	18	theme	cement	1056:1061	arg1	samples					1063:1069	cement samples	1056:1069	cement samples	1056:1069	Spindle-shaped osteoblasts with developed cytoplasmic membrane were found on the surfaces of cement samples after 7 days of culture.
24399509	6	19	theme	cells	764:768	arg1	viability					731:739	viability	731:739	viability	731:739	The viability and growth rate of the cells were similar for all CPCs, but better alkaline phosphatase activity was observed for CPC with CS and HA.
24399509	6	19	theme	cells	764:768	arg1	rate					752:755	growth rate	745:755	growth rate	745:755	The viability and growth rate of the cells were similar for all CPCs, but better alkaline phosphatase activity was observed for CPC with CS and HA.
24399509	5	20	dep	in	615:616	arg1	cements					631:637	cements	631:637	cements	631:637	The in vitro osteoblastic cell biocompatibility of in situ forming cements was evaluated and compared with those of conventional calcium phosphate cements (CPCs).
24399509	5	20	dep	in	615:616	arg1	situ					618:621	situ	618:621	situ	618:621	The in vitro osteoblastic cell biocompatibility of in situ forming cements was evaluated and compared with those of conventional calcium phosphate cements (CPCs).
24399509	0	21	theme	In	0:1	arg1	biocompatibility					9:24	In vitro biocompatibility	0:24	In vitro biocompatibility of chitosan/hyaluronic acid-containing calcium phosphate bone	0:86	In vitro biocompatibility of chitosan/hyaluronic acid-containing calcium phosphate bone cements.
24399509	4	22	theme	simulated	542:550	arg1	fluid					557:561	simulated body fluid	542:561	simulated body fluid	542:561	The acellular in vitro bioactivity revealed that different apatite morphologies were formed on the surfaces of cements after soaking in simulated body fluid.
24399509	5	23	theme	in	568:569	arg1	biocompatibility					595:610	The in vitro osteoblastic cell biocompatibility	564:610	The in vitro osteoblastic cell biocompatibility of in situ forming cements	564:637	The in vitro osteoblastic cell biocompatibility of in situ forming cements was evaluated and compared with those of conventional calcium phosphate cements (CPCs).
24399509	8	24	theme	cytoplasmic	1005:1015	arg1	membrane					1017:1024	developed cytoplasmic membrane	995:1024	developed cytoplasmic membrane	995:1024	Spindle-shaped osteoblasts with developed cytoplasmic membrane were found on the surfaces of cement samples after 7 days of culture.
24399509	5	25	theme	calcium	693:699	arg1	phosphate					701:709	conventional calcium phosphate cements	680:717	conventional calcium phosphate cements (CPCs)	680:724	The in vitro osteoblastic cell biocompatibility of in situ forming cements was evaluated and compared with those of conventional calcium phosphate cements (CPCs).
24399509	5	25	theme	calcium	693:699	arg1	CPCs					720:723	CPCs	720:723	CPCs	720:723	The in vitro osteoblastic cell biocompatibility of in situ forming cements was evaluated and compared with those of conventional calcium phosphate cements (CPCs).
24399509	8	26	theme	developed	995:1003	arg1	membrane					1017:1024	developed cytoplasmic membrane	995:1024	developed cytoplasmic membrane	995:1024	Spindle-shaped osteoblasts with developed cytoplasmic membrane were found on the surfaces of cement samples after 7 days of culture.
24399509	1	27	theme	bone	110:113	arg1	repair					115:120	bone repair	110:120	bone repair	110:120	The need for bone repair has increased as the population ages.
24399509	7	28	theme	cells	938:942	arg1	attachment					911:920	attachment	911:920	attachment of osteoblastic cells on their surfaces	911:960	Calcium phosphate cements supported attachment of osteoblastic cells on their surfaces.
24399509	3	29	dep	composition	284:294	arg1	The					280:282	The	280:282	The	280:282	The composition and morphological properties of cements were evaluated by X-ray diffraction and scanning electron microscopy.
24399509	2	30	theme	calcium	178:184	arg1	phosphate					186:194	calcium phosphate	178:194	calcium phosphate	178:194	In this research, calcium phosphate cements, with and without chitosan (CS) and hyaluronic acid (HA), were synthesized.
24399509	3	31	theme	electron	385:392	arg1	microscopy					394:403	scanning electron microscopy	376:403	scanning electron microscopy	376:403	The composition and morphological properties of cements were evaluated by X-ray diffraction and scanning electron microscopy.
24399509	4	32	theme	body	552:555	arg1	fluid					557:561	simulated body fluid	542:561	simulated body fluid	542:561	The acellular in vitro bioactivity revealed that different apatite morphologies were formed on the surfaces of cements after soaking in simulated body fluid.
24399509	8	33	theme	samples	1063:1069	arg1	surfaces					1044:1051	the surfaces	1040:1051	the surfaces of cement samples	1040:1069	Spindle-shaped osteoblasts with developed cytoplasmic membrane were found on the surfaces of cement samples after 7 days of culture.
24399509	0	34	theme	chitosan/hyaluronic	29:47	arg1	bone					83:86	chitosan/hyaluronic acid-containing calcium phosphate bone	29:86	chitosan/hyaluronic acid-containing calcium phosphate bone	29:86	In vitro biocompatibility of chitosan/hyaluronic acid-containing calcium phosphate bone cements.
24399509	4	35	theme	cements	517:523	arg1	surfaces					505:512	the surfaces	501:512	the surfaces of cements	501:523	The acellular in vitro bioactivity revealed that different apatite morphologies were formed on the surfaces of cements after soaking in simulated body fluid.
24399509	8	36	with	osteoblasts	978:988	arg1	membrane					1017:1024	developed cytoplasmic membrane	995:1024	developed cytoplasmic membrane	995:1024	Spindle-shaped osteoblasts with developed cytoplasmic membrane were found on the surfaces of cement samples after 7 days of culture.
24399509	0	37	theme	phosphate	73:81	arg1	bone					83:86	chitosan/hyaluronic acid-containing calcium phosphate bone	29:86	chitosan/hyaluronic acid-containing calcium phosphate bone	29:86	In vitro biocompatibility of chitosan/hyaluronic acid-containing calcium phosphate bone cements.
24399509	8	38	theme	culture	1087:1093	arg1	days					1079:1082	7 days	1077:1082	7 days of culture	1077:1093	Spindle-shaped osteoblasts with developed cytoplasmic membrane were found on the surfaces of cement samples after 7 days of culture.
24399509	0	39	theme	bone	83:86	arg1	biocompatibility					9:24	In vitro biocompatibility	0:24	In vitro biocompatibility of chitosan/hyaluronic acid-containing calcium phosphate bone	0:86	In vitro biocompatibility of chitosan/hyaluronic acid-containing calcium phosphate bone cements.
24399509	6	40	theme	growth	745:750	arg1	rate					752:755	growth rate	745:755	growth rate	745:755	The viability and growth rate of the cells were similar for all CPCs, but better alkaline phosphatase activity was observed for CPC with CS and HA.
24399509	3	41	theme	scanning	376:383	arg1	microscopy					394:403	scanning electron microscopy	376:403	scanning electron microscopy	376:403	The composition and morphological properties of cements were evaluated by X-ray diffraction and scanning electron microscopy.
24399509	6	42	theme	alkaline	808:815	arg1	phosphatase					817:827	better alkaline phosphatase	801:827	better alkaline phosphatase activity	801:836	The viability and growth rate of the cells were similar for all CPCs, but better alkaline phosphatase activity was observed for CPC with CS and HA.
24399509	7	43	dep	cements	893:899	arg1	supported					901:909	supported	901:909	cements supported attachment of osteoblastic cells on their surfaces	893:960	Calcium phosphate cements supported attachment of osteoblastic cells on their surfaces.
24399509	6	44	theme	better	801:806	arg1	phosphatase					817:827	better alkaline phosphatase	801:827	better alkaline phosphatase activity	801:836	The viability and growth rate of the cells were similar for all CPCs, but better alkaline phosphatase activity was observed for CPC with CS and HA.
24399509	2	45	theme	hyaluronic	240:249	arg1	HA					257:258	HA	257:258	HA	257:258	In this research, calcium phosphate cements, with and without chitosan (CS) and hyaluronic acid (HA), were synthesized.
24399509	2	45	theme	hyaluronic	240:249	arg1	acid					251:254	hyaluronic acid	240:254	hyaluronic acid (HA)	240:259	In this research, calcium phosphate cements, with and without chitosan (CS) and hyaluronic acid (HA), were synthesized.
24399509	7	46	theme	Calcium	875:881	arg1	phosphate					883:891	Calcium phosphate	875:891	Calcium phosphate	875:891	Calcium phosphate cements supported attachment of osteoblastic cells on their surfaces.
24399509	4	47	dep	in	420:421	arg1	vitro					423:427	vitro	423:427	vitro	423:427	The acellular in vitro bioactivity revealed that different apatite morphologies were formed on the surfaces of cements after soaking in simulated body fluid.
24399509	5	48	theme	conventional	680:691	arg1	phosphate					701:709	conventional calcium phosphate cements	680:717	conventional calcium phosphate cements (CPCs)	680:724	The in vitro osteoblastic cell biocompatibility of in situ forming cements was evaluated and compared with those of conventional calcium phosphate cements (CPCs).
24399509	5	48	theme	conventional	680:691	arg1	CPCs					720:723	CPCs	720:723	CPCs	720:723	The in vitro osteoblastic cell biocompatibility of in situ forming cements was evaluated and compared with those of conventional calcium phosphate cements (CPCs).
24399509	4	49	theme	in	420:421	arg1	bioactivity					429:439	The acellular in vitro bioactivity	406:439	The acellular in vitro bioactivity	406:439	The acellular in vitro bioactivity revealed that different apatite morphologies were formed on the surfaces of cements after soaking in simulated body fluid.
24399509	7	50	from	attachment	911:920	arg1	surfaces					953:960	their surfaces	947:960	their surfaces	947:960	Calcium phosphate cements supported attachment of osteoblastic cells on their surfaces.
24399509	4	51	theme	acellular	410:418	arg1	bioactivity					429:439	The acellular in vitro bioactivity	406:439	The acellular in vitro bioactivity	406:439	The acellular in vitro bioactivity revealed that different apatite morphologies were formed on the surfaces of cements after soaking in simulated body fluid.
24399509	1	52	theme	population	143:152	arg1	ages					154:157	the population ages	139:157	the population ages	139:157	The need for bone repair has increased as the population ages.
24399509	3	53	theme	morphological	300:312	arg1	properties					314:323	morphological properties	300:323	morphological properties	300:323	The composition and morphological properties of cements were evaluated by X-ray diffraction and scanning electron microscopy.
24399509	0	54	dep	In	0:1	arg1	vitro					3:7	vitro	3:7	vitro	3:7	In vitro biocompatibility of chitosan/hyaluronic acid-containing calcium phosphate bone cements.
24399509	9	55	theme	CPC-CS/HA	1138:1146	arg1	composites					1148:1157	the CPC-CS/HA composites	1134:1157	the CPC-CS/HA composites	1134:1157	These results reveal the potential of the CPC-CS/HA composites to be used in bone tissue engineering.
24399509	7	56	attach	attachment	911:920	arg3	surfaces					953:960	their surfaces	947:960	their surfaces	947:960	Calcium phosphate cements supported attachment of osteoblastic cells on their surfaces.
24399509	7	56	attach	attachment	911:920	arg2	cells					938:942	osteoblastic cells	925:942	osteoblastic cells	925:942	Calcium phosphate cements supported attachment of osteoblastic cells on their surfaces.
24399509	5	57	dep	in	568:569	arg1	vitro					571:575	vitro	571:575	vitro	571:575	The in vitro osteoblastic cell biocompatibility of in situ forming cements was evaluated and compared with those of conventional calcium phosphate cements (CPCs).
26876850	0	0	theme	optical	93:99	arg1	properties					101:110	Mechanical and optical properties	78:110	Well-aligned cellulose nanofiber-reinforced polyvinyl alcohol composite film: Mechanical and optical properties.	0:111	Well-aligned cellulose nanofiber-reinforced polyvinyl alcohol composite film: Mechanical and optical properties.
26876850	2	1	theme	mechanical-to-optical	535:555	arg1	properties					557:566	mechanical-to-optical properties	535:566	mechanical-to-optical properties of the CNFs-reinforced composites	535:600	We examined the effects of B-CA concentration and electrospinning parameters (e.g. spinning distance, and collection speed) on fiber morphology and orientation, which act on mechanical-to-optical properties of the CNFs-reinforced composites.
26876850	3	2	theme	improved	729:736	arg1	properties					749:758	improved mechanical properties	729:758	improved mechanical properties	729:758	Consequently, the resultant composite film exhibits high visible-light transmittance even with high fiber content, as well as improved mechanical properties.
26876850	3	3	theme	resultant	621:629	arg1	film					641:644	the resultant composite film	617:644	the resultant composite film	617:644	Consequently, the resultant composite film exhibits high visible-light transmittance even with high fiber content, as well as improved mechanical properties.
26876850	2	4	theme	electrospinning	411:425	arg1	parameters					427:436	electrospinning parameters	411:436	electrospinning parameters (e.g. spinning distance, and collection speed)	411:483	We examined the effects of B-CA concentration and electrospinning parameters (e.g. spinning distance, and collection speed) on fiber morphology and orientation, which act on mechanical-to-optical properties of the CNFs-reinforced composites.
26876850	1	5	used	used	291:294	arg2	nanofibers					142:151	Uniaxially aligned cellulose nanofibers	113:151	Uniaxially aligned cellulose nanofibers (CNFs)	113:158	Uniaxially aligned cellulose nanofibers (CNFs), which are fabricated by electrospinning of cellulose acetate derived from bamboo cellulose (B-CA) followed by deacetylation, were used as reinforcements to make optically transparent composite films.
26876850	1	5	used	used	291:294	arg2	reinforcements					299:312	reinforcements	299:312	reinforcements	299:312	Uniaxially aligned cellulose nanofibers (CNFs), which are fabricated by electrospinning of cellulose acetate derived from bamboo cellulose (B-CA) followed by deacetylation, were used as reinforcements to make optically transparent composite films.
26876850	1	5	used	used	291:294	arg2	CNFs					154:157	CNFs	154:157	CNFs	154:157	Uniaxially aligned cellulose nanofibers (CNFs), which are fabricated by electrospinning of cellulose acetate derived from bamboo cellulose (B-CA) followed by deacetylation, were used as reinforcements to make optically transparent composite films.
26876850	0	6	theme	composite	62:70	arg1	film					72:75	Well-aligned cellulose nanofiber-reinforced polyvinyl alcohol composite film	0:75	Well-aligned cellulose nanofiber-reinforced polyvinyl alcohol composite film: Mechanical and optical properties.	0:111	Well-aligned cellulose nanofiber-reinforced polyvinyl alcohol composite film: Mechanical and optical properties.
26876850	2	7	theme	collection	467:476	arg1	speed					478:482	collection speed	467:482	collection speed	467:482	We examined the effects of B-CA concentration and electrospinning parameters (e.g. spinning distance, and collection speed) on fiber morphology and orientation, which act on mechanical-to-optical properties of the CNFs-reinforced composites.
26876850	3	8	with	transmittance	674:686	arg1	properties					749:758	improved mechanical properties	729:758	improved mechanical properties	729:758	Consequently, the resultant composite film exhibits high visible-light transmittance even with high fiber content, as well as improved mechanical properties.
26876850	3	8	with	transmittance	674:686	arg1	content					709:715	high fiber content	698:715	high fiber content	698:715	Consequently, the resultant composite film exhibits high visible-light transmittance even with high fiber content, as well as improved mechanical properties.
26876850	2	9	theme	concentration	393:405	arg1	effects					377:383	the effects	373:383	the effects of B-CA concentration and electrospinning parameters (e.g. spinning distance, and collection speed) on fiber morphology and orientation, which act on mechanical-to-optical properties of the CNFs-reinforced composites	373:600	We examined the effects of B-CA concentration and electrospinning parameters (e.g. spinning distance, and collection speed) on fiber morphology and orientation, which act on mechanical-to-optical properties of the CNFs-reinforced composites.
26876850	0	10	theme	Well-aligned	0:11	arg1	film					72:75	Well-aligned cellulose nanofiber-reinforced polyvinyl alcohol composite film	0:75	Well-aligned cellulose nanofiber-reinforced polyvinyl alcohol composite film: Mechanical and optical properties.	0:111	Well-aligned cellulose nanofiber-reinforced polyvinyl alcohol composite film: Mechanical and optical properties.
26876850	2	11	theme	B-CA	388:391	arg1	concentration					393:405	B-CA concentration	388:405	B-CA concentration	388:405	We examined the effects of B-CA concentration and electrospinning parameters (e.g. spinning distance, and collection speed) on fiber morphology and orientation, which act on mechanical-to-optical properties of the CNFs-reinforced composites.
26876850	0	12	theme	polyvinyl	44:52	arg1	film					72:75	Well-aligned cellulose nanofiber-reinforced polyvinyl alcohol composite film	0:75	Well-aligned cellulose nanofiber-reinforced polyvinyl alcohol composite film: Mechanical and optical properties.	0:111	Well-aligned cellulose nanofiber-reinforced polyvinyl alcohol composite film: Mechanical and optical properties.
26876850	2	13	theme	CNFs-reinforced	575:589	arg1	composites					591:600	the CNFs-reinforced composites	571:600	the CNFs-reinforced composites	571:600	We examined the effects of B-CA concentration and electrospinning parameters (e.g. spinning distance, and collection speed) on fiber morphology and orientation, which act on mechanical-to-optical properties of the CNFs-reinforced composites.
26876850	2	14	from	effects	377:383	arg1	orientation					509:519	orientation	509:519	orientation	509:519	We examined the effects of B-CA concentration and electrospinning parameters (e.g. spinning distance, and collection speed) on fiber morphology and orientation, which act on mechanical-to-optical properties of the CNFs-reinforced composites.
26876850	2	14	from	effects	377:383	arg1	morphology					494:503	fiber morphology	488:503	fiber morphology	488:503	We examined the effects of B-CA concentration and electrospinning parameters (e.g. spinning distance, and collection speed) on fiber morphology and orientation, which act on mechanical-to-optical properties of the CNFs-reinforced composites.
26876850	1	15	theme	aligned	124:130	arg1	CNFs					154:157	CNFs	154:157	CNFs	154:157	Uniaxially aligned cellulose nanofibers (CNFs), which are fabricated by electrospinning of cellulose acetate derived from bamboo cellulose (B-CA) followed by deacetylation, were used as reinforcements to make optically transparent composite films.
26876850	1	15	theme	aligned	124:130	arg1	nanofibers					142:151	Uniaxially aligned cellulose nanofibers	113:151	Uniaxially aligned cellulose nanofibers (CNFs)	113:158	Uniaxially aligned cellulose nanofibers (CNFs), which are fabricated by electrospinning of cellulose acetate derived from bamboo cellulose (B-CA) followed by deacetylation, were used as reinforcements to make optically transparent composite films.
26876850	1	15	theme	aligned	124:130	arg1	reinforcements					299:312	reinforcements	299:312	reinforcements	299:312	Uniaxially aligned cellulose nanofibers (CNFs), which are fabricated by electrospinning of cellulose acetate derived from bamboo cellulose (B-CA) followed by deacetylation, were used as reinforcements to make optically transparent composite films.
26876850	2	16	theme	spinning	444:451	arg1	distance					453:460	spinning distance	444:460	spinning distance	444:460	We examined the effects of B-CA concentration and electrospinning parameters (e.g. spinning distance, and collection speed) on fiber morphology and orientation, which act on mechanical-to-optical properties of the CNFs-reinforced composites.
26876850	0	17	theme	nanofiber-reinforced	23:42	arg1	film					72:75	Well-aligned cellulose nanofiber-reinforced polyvinyl alcohol composite film	0:75	Well-aligned cellulose nanofiber-reinforced polyvinyl alcohol composite film: Mechanical and optical properties.	0:111	Well-aligned cellulose nanofiber-reinforced polyvinyl alcohol composite film: Mechanical and optical properties.
26876850	3	18	theme	high	698:701	arg1	content					709:715	high fiber content	698:715	high fiber content	698:715	Consequently, the resultant composite film exhibits high visible-light transmittance even with high fiber content, as well as improved mechanical properties.
26876850	1	19	theme	cellulose	132:140	arg1	CNFs					154:157	CNFs	154:157	CNFs	154:157	Uniaxially aligned cellulose nanofibers (CNFs), which are fabricated by electrospinning of cellulose acetate derived from bamboo cellulose (B-CA) followed by deacetylation, were used as reinforcements to make optically transparent composite films.
26876850	1	19	theme	cellulose	132:140	arg1	nanofibers					142:151	Uniaxially aligned cellulose nanofibers	113:151	Uniaxially aligned cellulose nanofibers (CNFs)	113:158	Uniaxially aligned cellulose nanofibers (CNFs), which are fabricated by electrospinning of cellulose acetate derived from bamboo cellulose (B-CA) followed by deacetylation, were used as reinforcements to make optically transparent composite films.
26876850	1	19	theme	cellulose	132:140	arg1	reinforcements					299:312	reinforcements	299:312	reinforcements	299:312	Uniaxially aligned cellulose nanofibers (CNFs), which are fabricated by electrospinning of cellulose acetate derived from bamboo cellulose (B-CA) followed by deacetylation, were used as reinforcements to make optically transparent composite films.
26876850	2	20	theme	fiber	488:492	arg1	morphology					494:503	fiber morphology	488:503	fiber morphology	488:503	We examined the effects of B-CA concentration and electrospinning parameters (e.g. spinning distance, and collection speed) on fiber morphology and orientation, which act on mechanical-to-optical properties of the CNFs-reinforced composites.
26876850	3	21	theme	fiber	703:707	arg1	content					709:715	high fiber content	698:715	high fiber content	698:715	Consequently, the resultant composite film exhibits high visible-light transmittance even with high fiber content, as well as improved mechanical properties.
26876850	3	22	theme	composite	631:639	arg1	film					641:644	the resultant composite film	617:644	the resultant composite film	617:644	Consequently, the resultant composite film exhibits high visible-light transmittance even with high fiber content, as well as improved mechanical properties.
26876850	1	23	theme	cellulose	204:212	arg1	acetate					214:220	cellulose acetate	204:220	cellulose acetate derived from bamboo cellulose (B-CA) followed by deacetylation	204:283	Uniaxially aligned cellulose nanofibers (CNFs), which are fabricated by electrospinning of cellulose acetate derived from bamboo cellulose (B-CA) followed by deacetylation, were used as reinforcements to make optically transparent composite films.
26876850	4	24	dep	optical	878:884	arg1	uses					886:889	uses	886:889	uses	886:889	The understanding obtained from this study may facilitate the development of novel nanofibrous materials for various optical uses.
26876850	0	25	theme	alcohol	54:60	arg1	film					72:75	Well-aligned cellulose nanofiber-reinforced polyvinyl alcohol composite film	0:75	Well-aligned cellulose nanofiber-reinforced polyvinyl alcohol composite film: Mechanical and optical properties.	0:111	Well-aligned cellulose nanofiber-reinforced polyvinyl alcohol composite film: Mechanical and optical properties.
26876850	0	26	dep	film	72:75	arg1	properties					101:110	Mechanical and optical properties	78:110	Well-aligned cellulose nanofiber-reinforced polyvinyl alcohol composite film: Mechanical and optical properties.	0:111	Well-aligned cellulose nanofiber-reinforced polyvinyl alcohol composite film: Mechanical and optical properties.
26876850	1	27	theme	bamboo	235:240	arg1	B-CA					253:256	B-CA	253:256	B-CA	253:256	Uniaxially aligned cellulose nanofibers (CNFs), which are fabricated by electrospinning of cellulose acetate derived from bamboo cellulose (B-CA) followed by deacetylation, were used as reinforcements to make optically transparent composite films.
26876850	1	27	theme	bamboo	235:240	arg1	cellulose					242:250	bamboo cellulose	235:250	bamboo cellulose (B-CA) followed by deacetylation	235:283	Uniaxially aligned cellulose nanofibers (CNFs), which are fabricated by electrospinning of cellulose acetate derived from bamboo cellulose (B-CA) followed by deacetylation, were used as reinforcements to make optically transparent composite films.
26876850	3	28	theme	mechanical	738:747	arg1	properties					749:758	improved mechanical properties	729:758	improved mechanical properties	729:758	Consequently, the resultant composite film exhibits high visible-light transmittance even with high fiber content, as well as improved mechanical properties.
26876850	1	29	attach	derived	222:228	arg1	B-CA					253:256	B-CA	253:256	B-CA	253:256	Uniaxially aligned cellulose nanofibers (CNFs), which are fabricated by electrospinning of cellulose acetate derived from bamboo cellulose (B-CA) followed by deacetylation, were used as reinforcements to make optically transparent composite films.
26876850	1	29	attach	derived	222:228	arg1	cellulose					242:250	bamboo cellulose	235:250	bamboo cellulose (B-CA) followed by deacetylation	235:283	Uniaxially aligned cellulose nanofibers (CNFs), which are fabricated by electrospinning of cellulose acetate derived from bamboo cellulose (B-CA) followed by deacetylation, were used as reinforcements to make optically transparent composite films.
26876850	1	29	attach	derived	222:228	arg2	acetate					214:220	cellulose acetate	204:220	cellulose acetate derived from bamboo cellulose (B-CA) followed by deacetylation	204:283	Uniaxially aligned cellulose nanofibers (CNFs), which are fabricated by electrospinning of cellulose acetate derived from bamboo cellulose (B-CA) followed by deacetylation, were used as reinforcements to make optically transparent composite films.
26876850	2	30	dep	distance	453:460	arg1	e.g.					439:442	e.g.	439:442	e.g.	439:442	We examined the effects of B-CA concentration and electrospinning parameters (e.g. spinning distance, and collection speed) on fiber morphology and orientation, which act on mechanical-to-optical properties of the CNFs-reinforced composites.
26876850	1	31	theme	transparent	332:342	arg1	films					354:358	optically transparent composite films	322:358	optically transparent composite films	322:358	Uniaxially aligned cellulose nanofibers (CNFs), which are fabricated by electrospinning of cellulose acetate derived from bamboo cellulose (B-CA) followed by deacetylation, were used as reinforcements to make optically transparent composite films.
26876850	4	32	theme	nanofibrous	844:854	arg1	materials					856:864	novel nanofibrous materials	838:864	novel nanofibrous materials	838:864	The understanding obtained from this study may facilitate the development of novel nanofibrous materials for various optical uses.
26876850	3	33	theme	visible-light	660:672	arg1	transmittance					674:686	high visible-light transmittance	655:686	high visible-light transmittance even with high fiber content, as well as improved mechanical properties	655:758	Consequently, the resultant composite film exhibits high visible-light transmittance even with high fiber content, as well as improved mechanical properties.
26876850	2	34	theme	composites	591:600	arg1	properties					557:566	mechanical-to-optical properties	535:566	mechanical-to-optical properties of the CNFs-reinforced composites	535:600	We examined the effects of B-CA concentration and electrospinning parameters (e.g. spinning distance, and collection speed) on fiber morphology and orientation, which act on mechanical-to-optical properties of the CNFs-reinforced composites.
26876850	4	35	theme	novel	838:842	arg1	materials					856:864	novel nanofibrous materials	838:864	novel nanofibrous materials	838:864	The understanding obtained from this study may facilitate the development of novel nanofibrous materials for various optical uses.
26876850	2	36	theme	parameters	427:436	arg1	effects					377:383	the effects	373:383	the effects of B-CA concentration and electrospinning parameters (e.g. spinning distance, and collection speed) on fiber morphology and orientation, which act on mechanical-to-optical properties of the CNFs-reinforced composites	373:600	We examined the effects of B-CA concentration and electrospinning parameters (e.g. spinning distance, and collection speed) on fiber morphology and orientation, which act on mechanical-to-optical properties of the CNFs-reinforced composites.
26876850	4	37	theme	materials	856:864	arg1	development					823:833	the development	819:833	the development of novel nanofibrous materials for various optical uses	819:889	The understanding obtained from this study may facilitate the development of novel nanofibrous materials for various optical uses.
26876850	1	38	theme	acetate	214:220	arg1	electrospinning					185:199	electrospinning	185:199	electrospinning of cellulose acetate derived from bamboo cellulose (B-CA) followed by deacetylation	185:283	Uniaxially aligned cellulose nanofibers (CNFs), which are fabricated by electrospinning of cellulose acetate derived from bamboo cellulose (B-CA) followed by deacetylation, were used as reinforcements to make optically transparent composite films.
26876850	0	39	theme	Mechanical	78:87	arg1	properties					101:110	Mechanical and optical properties	78:110	Well-aligned cellulose nanofiber-reinforced polyvinyl alcohol composite film: Mechanical and optical properties.	0:111	Well-aligned cellulose nanofiber-reinforced polyvinyl alcohol composite film: Mechanical and optical properties.
26876850	1	40	theme	composite	344:352	arg1	films					354:358	optically transparent composite films	322:358	optically transparent composite films	322:358	Uniaxially aligned cellulose nanofibers (CNFs), which are fabricated by electrospinning of cellulose acetate derived from bamboo cellulose (B-CA) followed by deacetylation, were used as reinforcements to make optically transparent composite films.
26876850	3	41	theme	high	655:658	arg1	transmittance					674:686	high visible-light transmittance	655:686	high visible-light transmittance even with high fiber content, as well as improved mechanical properties	655:758	Consequently, the resultant composite film exhibits high visible-light transmittance even with high fiber content, as well as improved mechanical properties.
26876850	0	42	theme	cellulose	13:21	arg1	film					72:75	Well-aligned cellulose nanofiber-reinforced polyvinyl alcohol composite film	0:75	Well-aligned cellulose nanofiber-reinforced polyvinyl alcohol composite film: Mechanical and optical properties.	0:111	Well-aligned cellulose nanofiber-reinforced polyvinyl alcohol composite film: Mechanical and optical properties.
26572466	3	0	theme	tensile	508:514	arg1	strength					516:523	tensile strength	508:523	tensile strength	508:523	The mechanical properties of the composite hydrogels with two different water contents were characterized by tensile test, the results shown that the tensile strength and elongation at break of composite hydrogels both increased obviously with increasing of ATP content.
26572466	3	1	from	break	543:547	arg1	at					540:541	elongation at break	529:547	elongation at break	529:547	The mechanical properties of the composite hydrogels with two different water contents were characterized by tensile test, the results shown that the tensile strength and elongation at break of composite hydrogels both increased obviously with increasing of ATP content.
26572466	3	1	from	break	543:547	arg1	elongation					529:538	elongation at break	529:547	elongation at break	529:547	The mechanical properties of the composite hydrogels with two different water contents were characterized by tensile test, the results shown that the tensile strength and elongation at break of composite hydrogels both increased obviously with increasing of ATP content.
26572466	6	2	theme	hydrogels	975:983	arg1	images					955:960	The SEM images	947:960	The SEM images of composite hydrogels	947:983	The SEM images of composite hydrogels shown quite different from the neat CS/GP hydrogel, where the pores were more tightly and with some uniform and smaller holes dispersed on the wall.
26572466	4	3	theme	strength	686:693	arg1	times					741:745	times	741:745	times	741:745	And, in our testing range, the maximum values of tensile strength and elongation at break were both more than 5 times larger than that of neat CS/GP hydrogel.
26572466	4	3	theme	strength	686:693	arg1	values					668:673	the maximum values	656:673	the maximum values of tensile strength and elongation at break	656:717	And, in our testing range, the maximum values of tensile strength and elongation at break were both more than 5 times larger than that of neat CS/GP hydrogel.
26572466	6	4	theme	composite	965:973	arg1	hydrogels					975:983	composite hydrogels	965:983	composite hydrogels	965:983	The SEM images of composite hydrogels shown quite different from the neat CS/GP hydrogel, where the pores were more tightly and with some uniform and smaller holes dispersed on the wall.
26572466	3	5	theme	mechanical	362:371	arg1	properties					373:382	The mechanical properties	358:382	The mechanical properties of the composite hydrogels with two different water contents	358:443	The mechanical properties of the composite hydrogels with two different water contents were characterized by tensile test, the results shown that the tensile strength and elongation at break of composite hydrogels both increased obviously with increasing of ATP content.
26572466	7	6	theme	CS	1294:1295	arg1	molecules					1297:1305	CS molecules	1294:1305	CS molecules	1294:1305	FT-IR test results revealed that the introduction of ATP increased the cross-link density because of the hydrogen bonds formation between ATP nanoparticles and CS molecules.
26572466	5	7	dep	transform	898:906	arg1	infrared					908:915	infrared	908:915	transform infrared spectroscopy testing (FT-IR)	898:944	We discussed this enhancement effect in detail by Scanning electron microscope observations (SEM) and Fourier transform infrared spectroscopy testing (FT-IR).
26572466	2	8	theme	ATP	312:314	arg1	particles					322:330	attapulgite (ATP) nano particles	299:330	attapulgite (ATP) nano particles	299:330	A novel nanocomposite injectable hydrogel was prepared by introducing attapulgite (ATP) nano particles into the CS/GP hydrogels.
26572466	8	9	from	impact	1329:1334	arg1	speed					1368:1372	gelation speed	1359:1372	gelation speed	1359:1372	Also, we studied the impact of ATP introduction on gelation speed through tracking the dynamic process of the sol-gel transition by means of rheological measurement, and the results shown that the reaction rate increased significantly with the increase of ATP concentration.
26572466	8	10	theme	concentration	1568:1580	arg1	increase					1552:1559	the increase	1548:1559	the increase of ATP concentration	1548:1580	Also, we studied the impact of ATP introduction on gelation speed through tracking the dynamic process of the sol-gel transition by means of rheological measurement, and the results shown that the reaction rate increased significantly with the increase of ATP concentration.
26572466	3	11	theme	composite	552:560	arg1	hydrogels					562:570	composite hydrogels	552:570	composite hydrogels	552:570	The mechanical properties of the composite hydrogels with two different water contents were characterized by tensile test, the results shown that the tensile strength and elongation at break of composite hydrogels both increased obviously with increasing of ATP content.
26572466	2	12	theme	attapulgite	299:309	arg1	particles					322:330	attapulgite (ATP) nano particles	299:330	attapulgite (ATP) nano particles	299:330	A novel nanocomposite injectable hydrogel was prepared by introducing attapulgite (ATP) nano particles into the CS/GP hydrogels.
26572466	7	13	theme	test	1140:1143	arg1	results					1145:1151	FT-IR test results	1134:1151	FT-IR test results	1134:1151	FT-IR test results revealed that the introduction of ATP increased the cross-link density because of the hydrogen bonds formation between ATP nanoparticles and CS molecules.
26572466	7	14	theme	cross-link	1205:1214	arg1	density					1216:1222	the cross-link density	1201:1222	the cross-link density	1201:1222	FT-IR test results revealed that the introduction of ATP increased the cross-link density because of the hydrogen bonds formation between ATP nanoparticles and CS molecules.
26572466	4	15	from	times	741:745	arg1	range					649:653	our testing range	637:653	our testing range	637:653	And, in our testing range, the maximum values of tensile strength and elongation at break were both more than 5 times larger than that of neat CS/GP hydrogel.
26572466	3	16	theme	at	540:541	arg1	break					543:547	elongation at break	529:547	elongation at break	529:547	The mechanical properties of the composite hydrogels with two different water contents were characterized by tensile test, the results shown that the tensile strength and elongation at break of composite hydrogels both increased obviously with increasing of ATP content.
26572466	5	17	theme	electron	847:854	arg1	observations					867:878	electron microscope observations	847:878	Scanning electron microscope observations (SEM)	838:884	We discussed this enhancement effect in detail by Scanning electron microscope observations (SEM) and Fourier transform infrared spectroscopy testing (FT-IR).
26572466	1	18	theme	chitosan/β-glycerophosphate	157:183	arg1	hydrogels					218:226	chitosan/β-glycerophosphate disodium salt (CS/GP) injectable hydrogels	157:226	chitosan/β-glycerophosphate disodium salt (CS/GP) injectable hydrogels	157:226	The aim of the current study was to enhance the mechanical property of chitosan/β-glycerophosphate disodium salt (CS/GP) injectable hydrogels.
26572466	3	19	theme	different	420:428	arg1	contents					436:443	two different water contents	416:443	two different water contents	416:443	The mechanical properties of the composite hydrogels with two different water contents were characterized by tensile test, the results shown that the tensile strength and elongation at break of composite hydrogels both increased obviously with increasing of ATP content.
26572466	7	20	theme	ATP	1272:1274	arg1	nanoparticles					1276:1288	ATP nanoparticles	1272:1288	ATP nanoparticles	1272:1288	FT-IR test results revealed that the introduction of ATP increased the cross-link density because of the hydrogen bonds formation between ATP nanoparticles and CS molecules.
26572466	5	21	dep	Fourier	890:896	arg1	transform					898:906	transform	898:906	transform infrared spectroscopy testing (FT-IR)	898:944	We discussed this enhancement effect in detail by Scanning electron microscope observations (SEM) and Fourier transform infrared spectroscopy testing (FT-IR).
26572466	1	22	theme	disodium	185:192	arg1	hydrogels					218:226	chitosan/β-glycerophosphate disodium salt (CS/GP) injectable hydrogels	157:226	chitosan/β-glycerophosphate disodium salt (CS/GP) injectable hydrogels	157:226	The aim of the current study was to enhance the mechanical property of chitosan/β-glycerophosphate disodium salt (CS/GP) injectable hydrogels.
26572466	3	23	theme	composite	391:399	arg1	hydrogels					401:409	the composite hydrogels	387:409	the composite hydrogels with two different water contents	387:443	The mechanical properties of the composite hydrogels with two different water contents were characterized by tensile test, the results shown that the tensile strength and elongation at break of composite hydrogels both increased obviously with increasing of ATP content.
26572466	8	24	theme	reaction	1505:1512	arg1	rate					1514:1517	the reaction rate	1501:1517	the reaction rate	1501:1517	Also, we studied the impact of ATP introduction on gelation speed through tracking the dynamic process of the sol-gel transition by means of rheological measurement, and the results shown that the reaction rate increased significantly with the increase of ATP concentration.
26572466	7	25	theme	bonds	1248:1252	arg1	formation					1254:1262	the hydrogen bonds formation	1235:1262	the hydrogen bonds formation between ATP nanoparticles and CS molecules	1235:1305	FT-IR test results revealed that the introduction of ATP increased the cross-link density because of the hydrogen bonds formation between ATP nanoparticles and CS molecules.
26572466	5	26	theme	spectroscopy	917:928	arg1	FT-IR					939:943	FT-IR	939:943	FT-IR	939:943	We discussed this enhancement effect in detail by Scanning electron microscope observations (SEM) and Fourier transform infrared spectroscopy testing (FT-IR).
26572466	5	26	theme	spectroscopy	917:928	arg1	testing					930:936	spectroscopy testing	917:936	spectroscopy testing (FT-IR)	917:944	We discussed this enhancement effect in detail by Scanning electron microscope observations (SEM) and Fourier transform infrared spectroscopy testing (FT-IR).
26572466	8	27	theme	ATP	1564:1566	arg1	concentration					1568:1580	ATP concentration	1564:1580	ATP concentration	1564:1580	Also, we studied the impact of ATP introduction on gelation speed through tracking the dynamic process of the sol-gel transition by means of rheological measurement, and the results shown that the reaction rate increased significantly with the increase of ATP concentration.
26572466	1	28	theme	salt	194:197	arg1	hydrogels					218:226	chitosan/β-glycerophosphate disodium salt (CS/GP) injectable hydrogels	157:226	chitosan/β-glycerophosphate disodium salt (CS/GP) injectable hydrogels	157:226	The aim of the current study was to enhance the mechanical property of chitosan/β-glycerophosphate disodium salt (CS/GP) injectable hydrogels.
26572466	3	29	theme	hydrogels	401:409	arg1	properties					373:382	The mechanical properties	358:382	The mechanical properties of the composite hydrogels with two different water contents	358:443	The mechanical properties of the composite hydrogels with two different water contents were characterized by tensile test, the results shown that the tensile strength and elongation at break of composite hydrogels both increased obviously with increasing of ATP content.
26572466	6	30	theme	SEM	951:953	arg1	images					955:960	The SEM images	947:960	The SEM images of composite hydrogels	947:983	The SEM images of composite hydrogels shown quite different from the neat CS/GP hydrogel, where the pores were more tightly and with some uniform and smaller holes dispersed on the wall.
26572466	6	31	from	hydrogel	1027:1034	arg1	different					997:1005	different	997:1005	different	997:1005	The SEM images of composite hydrogels shown quite different from the neat CS/GP hydrogel, where the pores were more tightly and with some uniform and smaller holes dispersed on the wall.
26572466	2	32	theme	nano	317:320	arg1	particles					322:330	attapulgite (ATP) nano particles	299:330	attapulgite (ATP) nano particles	299:330	A novel nanocomposite injectable hydrogel was prepared by introducing attapulgite (ATP) nano particles into the CS/GP hydrogels.
26572466	4	33	theme	maximum	660:666	arg1	times					741:745	times	741:745	times	741:745	And, in our testing range, the maximum values of tensile strength and elongation at break were both more than 5 times larger than that of neat CS/GP hydrogel.
26572466	4	33	theme	maximum	660:666	arg1	values					668:673	the maximum values	656:673	the maximum values of tensile strength and elongation at break	656:717	And, in our testing range, the maximum values of tensile strength and elongation at break were both more than 5 times larger than that of neat CS/GP hydrogel.
26572466	3	34	theme	hydrogels	562:570	arg1	strength					516:523	tensile strength	508:523	tensile strength	508:523	The mechanical properties of the composite hydrogels with two different water contents were characterized by tensile test, the results shown that the tensile strength and elongation at break of composite hydrogels both increased obviously with increasing of ATP content.
26572466	3	34	theme	hydrogels	562:570	arg1	break					543:547	elongation at break	529:547	elongation at break	529:547	The mechanical properties of the composite hydrogels with two different water contents were characterized by tensile test, the results shown that the tensile strength and elongation at break of composite hydrogels both increased obviously with increasing of ATP content.
26572466	8	35	theme	introduction	1343:1354	arg1	impact					1329:1334	the impact	1325:1334	the impact of ATP introduction on gelation speed	1325:1372	Also, we studied the impact of ATP introduction on gelation speed through tracking the dynamic process of the sol-gel transition by means of rheological measurement, and the results shown that the reaction rate increased significantly with the increase of ATP concentration.
26572466	1	36	theme	CS/GP	200:204	arg1	hydrogels					218:226	chitosan/β-glycerophosphate disodium salt (CS/GP) injectable hydrogels	157:226	chitosan/β-glycerophosphate disodium salt (CS/GP) injectable hydrogels	157:226	The aim of the current study was to enhance the mechanical property of chitosan/β-glycerophosphate disodium salt (CS/GP) injectable hydrogels.
26572466	3	37	dep	strength	516:523	arg1	the					504:506	the	504:506	the	504:506	The mechanical properties of the composite hydrogels with two different water contents were characterized by tensile test, the results shown that the tensile strength and elongation at break of composite hydrogels both increased obviously with increasing of ATP content.
26572466	8	38	theme	ATP	1339:1341	arg1	introduction					1343:1354	ATP introduction	1339:1354	ATP introduction	1339:1354	Also, we studied the impact of ATP introduction on gelation speed through tracking the dynamic process of the sol-gel transition by means of rheological measurement, and the results shown that the reaction rate increased significantly with the increase of ATP concentration.
26572466	1	39	theme	current	101:107	arg1	study					109:113	the current study	97:113	the current study	97:113	The aim of the current study was to enhance the mechanical property of chitosan/β-glycerophosphate disodium salt (CS/GP) injectable hydrogels.
26572466	6	40	theme	CS/GP	1021:1025	arg1	hydrogel					1027:1034	the neat CS/GP hydrogel	1012:1034	the neat CS/GP hydrogel	1012:1034	The SEM images of composite hydrogels shown quite different from the neat CS/GP hydrogel, where the pores were more tightly and with some uniform and smaller holes dispersed on the wall.
26572466	0	41	theme	chitosan	36:43	arg1	characterization					14:29	characterization	14:29	characterization	14:29	Synthesis and characterization of a chitosan based nanocomposite injectable hydrogel.
26572466	0	41	theme	chitosan	36:43	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis and characterization of a chitosan based nanocomposite injectable hydrogel.
26572466	4	42	theme	elongation	699:708	arg1	times					741:745	times	741:745	times	741:745	And, in our testing range, the maximum values of tensile strength and elongation at break were both more than 5 times larger than that of neat CS/GP hydrogel.
26572466	4	42	theme	elongation	699:708	arg1	values					668:673	the maximum values	656:673	the maximum values of tensile strength and elongation at break	656:717	And, in our testing range, the maximum values of tensile strength and elongation at break were both more than 5 times larger than that of neat CS/GP hydrogel.
26572466	3	43	theme	tensile	467:473	arg1	test					475:478	tensile test	467:478	tensile test	467:478	The mechanical properties of the composite hydrogels with two different water contents were characterized by tensile test, the results shown that the tensile strength and elongation at break of composite hydrogels both increased obviously with increasing of ATP content.
26572466	2	44	theme	nanocomposite	237:249	arg1	hydrogel					262:269	A novel nanocomposite injectable hydrogel	229:269	A novel nanocomposite injectable hydrogel	229:269	A novel nanocomposite injectable hydrogel was prepared by introducing attapulgite (ATP) nano particles into the CS/GP hydrogels.
26572466	5	45	theme	microscope	856:865	arg1	observations					867:878	electron microscope observations	847:878	Scanning electron microscope observations (SEM)	838:884	We discussed this enhancement effect in detail by Scanning electron microscope observations (SEM) and Fourier transform infrared spectroscopy testing (FT-IR).
26572466	1	46	theme	injectable	207:216	arg1	hydrogels					218:226	chitosan/β-glycerophosphate disodium salt (CS/GP) injectable hydrogels	157:226	chitosan/β-glycerophosphate disodium salt (CS/GP) injectable hydrogels	157:226	The aim of the current study was to enhance the mechanical property of chitosan/β-glycerophosphate disodium salt (CS/GP) injectable hydrogels.
26572466	3	47	theme	water	430:434	arg1	contents					436:443	two different water contents	416:443	two different water contents	416:443	The mechanical properties of the composite hydrogels with two different water contents were characterized by tensile test, the results shown that the tensile strength and elongation at break of composite hydrogels both increased obviously with increasing of ATP content.
26572466	3	48	theme	ATP	616:618	arg1	content					620:626	ATP content	616:626	ATP content	616:626	The mechanical properties of the composite hydrogels with two different water contents were characterized by tensile test, the results shown that the tensile strength and elongation at break of composite hydrogels both increased obviously with increasing of ATP content.
26572466	1	49	theme	hydrogels	218:226	arg1	property					145:152	the mechanical property	130:152	the mechanical property of chitosan/β-glycerophosphate disodium salt (CS/GP) injectable hydrogels	130:226	The aim of the current study was to enhance the mechanical property of chitosan/β-glycerophosphate disodium salt (CS/GP) injectable hydrogels.
26572466	7	50	theme	ATP	1187:1189	arg1	introduction					1171:1182	the introduction	1167:1182	the introduction of ATP	1167:1189	FT-IR test results revealed that the introduction of ATP increased the cross-link density because of the hydrogen bonds formation between ATP nanoparticles and CS molecules.
26572466	8	51	theme	gelation	1359:1366	arg1	speed					1368:1372	gelation speed	1359:1372	gelation speed	1359:1372	Also, we studied the impact of ATP introduction on gelation speed through tracking the dynamic process of the sol-gel transition by means of rheological measurement, and the results shown that the reaction rate increased significantly with the increase of ATP concentration.
26572466	6	52	theme	uniform	1085:1091	arg1	holes					1105:1109	some uniform and smaller holes	1080:1109	some uniform and smaller holes dispersed on the wall	1080:1131	The SEM images of composite hydrogels shown quite different from the neat CS/GP hydrogel, where the pores were more tightly and with some uniform and smaller holes dispersed on the wall.
26572466	1	53	theme	study	109:113	arg1	aim					90:92	The aim	86:92	The aim of the current study	86:113	The aim of the current study was to enhance the mechanical property of chitosan/β-glycerophosphate disodium salt (CS/GP) injectable hydrogels.
26572466	8	54	theme	rheological	1449:1459	arg1	measurement					1461:1471	rheological measurement	1449:1471	rheological measurement	1449:1471	Also, we studied the impact of ATP introduction on gelation speed through tracking the dynamic process of the sol-gel transition by means of rheological measurement, and the results shown that the reaction rate increased significantly with the increase of ATP concentration.
26572466	8	55	theme	transition	1426:1435	arg1	process					1403:1409	the dynamic process	1391:1409	the dynamic process of the sol-gel transition	1391:1435	Also, we studied the impact of ATP introduction on gelation speed through tracking the dynamic process of the sol-gel transition by means of rheological measurement, and the results shown that the reaction rate increased significantly with the increase of ATP concentration.
26572466	5	56	dep	Scanning	838:845	arg1	observations					867:878	electron microscope observations	847:878	Scanning electron microscope observations (SEM)	838:884	We discussed this enhancement effect in detail by Scanning electron microscope observations (SEM) and Fourier transform infrared spectroscopy testing (FT-IR).
26572466	2	57	theme	CS/GP	341:345	arg1	hydrogels					347:355	the CS/GP hydrogels	337:355	the CS/GP hydrogels	337:355	A novel nanocomposite injectable hydrogel was prepared by introducing attapulgite (ATP) nano particles into the CS/GP hydrogels.
26572466	8	58	theme	dynamic	1395:1401	arg1	process					1403:1409	the dynamic process	1391:1409	the dynamic process of the sol-gel transition	1391:1435	Also, we studied the impact of ATP introduction on gelation speed through tracking the dynamic process of the sol-gel transition by means of rheological measurement, and the results shown that the reaction rate increased significantly with the increase of ATP concentration.
26572466	4	59	theme	CS/GP	772:776	arg1	hydrogel					778:785	neat CS/GP hydrogel	767:785	neat CS/GP hydrogel	767:785	And, in our testing range, the maximum values of tensile strength and elongation at break were both more than 5 times larger than that of neat CS/GP hydrogel.
26572466	4	60	dep	times	741:745	arg1	larger					747:752	larger	747:752	larger	747:752	And, in our testing range, the maximum values of tensile strength and elongation at break were both more than 5 times larger than that of neat CS/GP hydrogel.
26572466	4	60	dep	times	741:745	arg1	both					724:727	both	724:727	both	724:727	And, in our testing range, the maximum values of tensile strength and elongation at break were both more than 5 times larger than that of neat CS/GP hydrogel.
26572466	8	61	theme	sol-gel	1418:1424	arg1	transition					1426:1435	the sol-gel transition	1414:1435	the sol-gel transition	1414:1435	Also, we studied the impact of ATP introduction on gelation speed through tracking the dynamic process of the sol-gel transition by means of rheological measurement, and the results shown that the reaction rate increased significantly with the increase of ATP concentration.
26572466	4	62	theme	neat	767:770	arg1	hydrogel					778:785	neat CS/GP hydrogel	767:785	neat CS/GP hydrogel	767:785	And, in our testing range, the maximum values of tensile strength and elongation at break were both more than 5 times larger than that of neat CS/GP hydrogel.
26572466	2	63	theme	novel	231:235	arg1	hydrogel					262:269	A novel nanocomposite injectable hydrogel	229:269	A novel nanocomposite injectable hydrogel	229:269	A novel nanocomposite injectable hydrogel was prepared by introducing attapulgite (ATP) nano particles into the CS/GP hydrogels.
26572466	4	64	theme	5	739:739	arg1	times					741:745	times	741:745	times	741:745	And, in our testing range, the maximum values of tensile strength and elongation at break were both more than 5 times larger than that of neat CS/GP hydrogel.
26572466	4	64	theme	5	739:739	arg1	values					668:673	the maximum values	656:673	the maximum values of tensile strength and elongation at break	656:717	And, in our testing range, the maximum values of tensile strength and elongation at break were both more than 5 times larger than that of neat CS/GP hydrogel.
26572466	3	65	with	hydrogels	401:409	arg1	contents					436:443	two different water contents	416:443	two different water contents	416:443	The mechanical properties of the composite hydrogels with two different water contents were characterized by tensile test, the results shown that the tensile strength and elongation at break of composite hydrogels both increased obviously with increasing of ATP content.
26572466	7	66	theme	hydrogen	1239:1246	arg1	bonds					1248:1252	hydrogen bonds	1239:1252	the hydrogen bonds formation between ATP nanoparticles and CS molecules	1235:1305	FT-IR test results revealed that the introduction of ATP increased the cross-link density because of the hydrogen bonds formation between ATP nanoparticles and CS molecules.
26572466	1	67	theme	mechanical	134:143	arg1	property					145:152	the mechanical property	130:152	the mechanical property of chitosan/β-glycerophosphate disodium salt (CS/GP) injectable hydrogels	130:226	The aim of the current study was to enhance the mechanical property of chitosan/β-glycerophosphate disodium salt (CS/GP) injectable hydrogels.
26572466	4	68	theme	testing	641:647	arg1	range					649:653	our testing range	637:653	our testing range	637:653	And, in our testing range, the maximum values of tensile strength and elongation at break were both more than 5 times larger than that of neat CS/GP hydrogel.
26572466	5	69	theme	enhancement	806:816	arg1	effect					818:823	this enhancement effect	801:823	this enhancement effect	801:823	We discussed this enhancement effect in detail by Scanning electron microscope observations (SEM) and Fourier transform infrared spectroscopy testing (FT-IR).
26572466	4	70	theme	tensile	678:684	arg1	strength					686:693	tensile strength	678:693	tensile strength	678:693	And, in our testing range, the maximum values of tensile strength and elongation at break were both more than 5 times larger than that of neat CS/GP hydrogel.
26572466	4	71	from	break	713:717	arg1	times					741:745	times	741:745	times	741:745	And, in our testing range, the maximum values of tensile strength and elongation at break were both more than 5 times larger than that of neat CS/GP hydrogel.
26572466	4	71	from	break	713:717	arg1	values					668:673	the maximum values	656:673	the maximum values of tensile strength and elongation at break	656:717	And, in our testing range, the maximum values of tensile strength and elongation at break were both more than 5 times larger than that of neat CS/GP hydrogel.
26572466	3	72	dep	shown	493:497	arg1	both					572:575	both	572:575	both	572:575	The mechanical properties of the composite hydrogels with two different water contents were characterized by tensile test, the results shown that the tensile strength and elongation at break of composite hydrogels both increased obviously with increasing of ATP content.
26572466	0	73	theme	injectable	65:74	arg1	hydrogel					76:83	injectable hydrogel	65:83	injectable hydrogel	65:83	Synthesis and characterization of a chitosan based nanocomposite injectable hydrogel.
26572466	6	74	theme	smaller	1097:1103	arg1	holes					1105:1109	some uniform and smaller holes	1080:1109	some uniform and smaller holes dispersed on the wall	1080:1131	The SEM images of composite hydrogels shown quite different from the neat CS/GP hydrogel, where the pores were more tightly and with some uniform and smaller holes dispersed on the wall.
26572466	2	75	theme	injectable	251:260	arg1	hydrogel					262:269	A novel nanocomposite injectable hydrogel	229:269	A novel nanocomposite injectable hydrogel	229:269	A novel nanocomposite injectable hydrogel was prepared by introducing attapulgite (ATP) nano particles into the CS/GP hydrogels.
26572466	3	76	theme	elongation	529:538	arg1	break					543:547	elongation at break	529:547	elongation at break	529:547	The mechanical properties of the composite hydrogels with two different water contents were characterized by tensile test, the results shown that the tensile strength and elongation at break of composite hydrogels both increased obviously with increasing of ATP content.
26572466	7	77	theme	FT-IR	1134:1138	arg1	results					1145:1151	FT-IR test results	1134:1151	FT-IR test results	1134:1151	FT-IR test results revealed that the introduction of ATP increased the cross-link density because of the hydrogen bonds formation between ATP nanoparticles and CS molecules.
26572466	6	78	theme	neat	1016:1019	arg1	hydrogel					1027:1034	the neat CS/GP hydrogel	1012:1034	the neat CS/GP hydrogel	1012:1034	The SEM images of composite hydrogels shown quite different from the neat CS/GP hydrogel, where the pores were more tightly and with some uniform and smaller holes dispersed on the wall.
27423880	5	0	theme	serotype	1032:1039	arg1	sequence					1047:1054	S. suis serotype 9 CPS sequence	1024:1054	S. suis serotype 9 CPS sequence	1024:1054	A correlation between S. suis serotype 9 CPS sequence and genes of this serotype cps locus encoding putative glycosyltransferases and polymerase responsible for the biosynthesis of the repeating unit was tentatively established.
27423880	4	1	contain	contain	850:856	arg1	CPS					837:839	serotype 9 CPS	826:839	serotype 9 CPS	826:839	Chemical and spectroscopic data gave the repeating unit sequence: [3)Glcol-6-P-3-[D-Gal(α1-2)]D-Gal(β1-3)D-Sug(β1-3)L-Rha(α1-)]n. Compared to previously characterized S. suis CPSs (serotypes 1, 1/2, 2 and 14), serotype 9 CPS does not contain sialic acid but contains a labile 4-keto sugar (2-acetamido-2,6-dideoxy-β-D-xylo-hexopyranos-4-ulose), one particular feature of this serotype.
27423880	4	1	contain	contain	850:856	arg2	acid					865:868	sialic acid	858:868	sialic acid	858:868	Chemical and spectroscopic data gave the repeating unit sequence: [3)Glcol-6-P-3-[D-Gal(α1-2)]D-Gal(β1-3)D-Sug(β1-3)L-Rha(α1-)]n. Compared to previously characterized S. suis CPSs (serotypes 1, 1/2, 2 and 14), serotype 9 CPS does not contain sialic acid but contains a labile 4-keto sugar (2-acetamido-2,6-dideoxy-β-D-xylo-hexopyranos-4-ulose), one particular feature of this serotype.
27423880	4	2	theme	sialic	858:863	arg1	acid					865:868	sialic acid	858:868	sialic acid	858:868	Chemical and spectroscopic data gave the repeating unit sequence: [3)Glcol-6-P-3-[D-Gal(α1-2)]D-Gal(β1-3)D-Sug(β1-3)L-Rha(α1-)]n. Compared to previously characterized S. suis CPSs (serotypes 1, 1/2, 2 and 14), serotype 9 CPS does not contain sialic acid but contains a labile 4-keto sugar (2-acetamido-2,6-dideoxy-β-D-xylo-hexopyranos-4-ulose), one particular feature of this serotype.
27423880	1	3	theme	Streptococcus	162:174	arg1	serotype					222:229	the most prevalent S. suis serotype	195:229	the most prevalent S. suis serotype in several European countries	195:259	Streptococcus suis serotype 9 is the most prevalent S. suis serotype in several European countries.
27423880	1	3	theme	Streptococcus	162:174	arg1	serotype					181:188	Streptococcus suis serotype 9	162:190	Streptococcus suis serotype 9	162:190	Streptococcus suis serotype 9 is the most prevalent S. suis serotype in several European countries.
27423880	4	4	theme	particular	965:974	arg1	sugar					899:903	a labile 4-keto sugar	883:903	a labile 4-keto sugar (2-acetamido-2,6-dideoxy-β-D-xylo-hexopyranos-4-ulose)	883:958	Chemical and spectroscopic data gave the repeating unit sequence: [3)Glcol-6-P-3-[D-Gal(α1-2)]D-Gal(β1-3)D-Sug(β1-3)L-Rha(α1-)]n. Compared to previously characterized S. suis CPSs (serotypes 1, 1/2, 2 and 14), serotype 9 CPS does not contain sialic acid but contains a labile 4-keto sugar (2-acetamido-2,6-dideoxy-β-D-xylo-hexopyranos-4-ulose), one particular feature of this serotype.
27423880	4	4	theme	particular	965:974	arg1	feature					976:982	one particular feature	961:982	one particular feature	961:982	Chemical and spectroscopic data gave the repeating unit sequence: [3)Glcol-6-P-3-[D-Gal(α1-2)]D-Gal(β1-3)D-Sug(β1-3)L-Rha(α1-)]n. Compared to previously characterized S. suis CPSs (serotypes 1, 1/2, 2 and 14), serotype 9 CPS does not contain sialic acid but contains a labile 4-keto sugar (2-acetamido-2,6-dideoxy-β-D-xylo-hexopyranos-4-ulose), one particular feature of this serotype.
27423880	4	5	theme	D-Gal	710:714	arg1	α1-					738:740	α1-	738:740	α1-	738:740	Chemical and spectroscopic data gave the repeating unit sequence: [3)Glcol-6-P-3-[D-Gal(α1-2)]D-Gal(β1-3)D-Sug(β1-3)L-Rha(α1-)]n. Compared to previously characterized S. suis CPSs (serotypes 1, 1/2, 2 and 14), serotype 9 CPS does not contain sialic acid but contains a labile 4-keto sugar (2-acetamido-2,6-dideoxy-β-D-xylo-hexopyranos-4-ulose), one particular feature of this serotype.
27423880	4	5	theme	D-Gal	710:714	arg1	L-Rha					732:736	[3)Glcol-6-P-3-[D-Gal(α1-2)]D-Gal(β1-3)D-Sug(β1-3)L-Rha	682:736	[3)Glcol-6-P-3-[D-Gal(α1-2)]D-Gal(β1-3)D-Sug(β1-3)L-Rha(α1-)]n.	682:744	Chemical and spectroscopic data gave the repeating unit sequence: [3)Glcol-6-P-3-[D-Gal(α1-2)]D-Gal(β1-3)D-Sug(β1-3)L-Rha(α1-)]n. Compared to previously characterized S. suis CPSs (serotypes 1, 1/2, 2 and 14), serotype 9 CPS does not contain sialic acid but contains a labile 4-keto sugar (2-acetamido-2,6-dideoxy-β-D-xylo-hexopyranos-4-ulose), one particular feature of this serotype.
27423880	5	6	theme	CPS	1043:1045	arg1	sequence					1047:1054	S. suis serotype 9 CPS sequence	1024:1054	S. suis serotype 9 CPS sequence	1024:1054	A correlation between S. suis serotype 9 CPS sequence and genes of this serotype cps locus encoding putative glycosyltransferases and polymerase responsible for the biosynthesis of the repeating unit was tentatively established.
27423880	3	7	theme	virulence	527:535	arg1	polysaccharide					487:500	serotype 9 capsular polysaccharide	467:500	serotype 9 capsular polysaccharide (CPS)	467:506	Here we determined for the first time the chemical composition and structure of serotype 9 capsular polysaccharide (CPS), a major bacterial virulence factor and the antigen at the origin of S. suis classification into serotypes.
27423880	3	7	theme	virulence	527:535	arg1	factor					537:542	a major bacterial virulence factor	509:542	a major bacterial virulence factor	509:542	Here we determined for the first time the chemical composition and structure of serotype 9 capsular polysaccharide (CPS), a major bacterial virulence factor and the antigen at the origin of S. suis classification into serotypes.
27423880	5	8	theme	repeating	1187:1195	arg1	unit					1197:1200	the repeating unit	1183:1200	the repeating unit	1183:1200	A correlation between S. suis serotype 9 CPS sequence and genes of this serotype cps locus encoding putative glycosyltransferases and polymerase responsible for the biosynthesis of the repeating unit was tentatively established.
27423880	0	9	theme	functions	99:107	arg1	determination					10:22	Structure determination	0:22	Structure determination of Streptococcus suis serotype 9 capsular polysaccharide and assignment of functions of the cps locus genes involved in its biosynthesis.	0:160	Structure determination of Streptococcus suis serotype 9 capsular polysaccharide and assignment of functions of the cps locus genes involved in its biosynthesis.
27423880	4	10	theme	Chemical	616:623	arg1	data					643:646	Chemical and spectroscopic data	616:646	Chemical and spectroscopic data	616:646	Chemical and spectroscopic data gave the repeating unit sequence: [3)Glcol-6-P-3-[D-Gal(α1-2)]D-Gal(β1-3)D-Sug(β1-3)L-Rha(α1-)]n. Compared to previously characterized S. suis CPSs (serotypes 1, 1/2, 2 and 14), serotype 9 CPS does not contain sialic acid but contains a labile 4-keto sugar (2-acetamido-2,6-dideoxy-β-D-xylo-hexopyranos-4-ulose), one particular feature of this serotype.
27423880	4	11	theme	4-keto	892:897	arg1	sugar					899:903	a labile 4-keto sugar	883:903	a labile 4-keto sugar (2-acetamido-2,6-dideoxy-β-D-xylo-hexopyranos-4-ulose)	883:958	Chemical and spectroscopic data gave the repeating unit sequence: [3)Glcol-6-P-3-[D-Gal(α1-2)]D-Gal(β1-3)D-Sug(β1-3)L-Rha(α1-)]n. Compared to previously characterized S. suis CPSs (serotypes 1, 1/2, 2 and 14), serotype 9 CPS does not contain sialic acid but contains a labile 4-keto sugar (2-acetamido-2,6-dideoxy-β-D-xylo-hexopyranos-4-ulose), one particular feature of this serotype.
27423880	4	11	theme	4-keto	892:897	arg1	feature					976:982	one particular feature	961:982	one particular feature	961:982	Chemical and spectroscopic data gave the repeating unit sequence: [3)Glcol-6-P-3-[D-Gal(α1-2)]D-Gal(β1-3)D-Sug(β1-3)L-Rha(α1-)]n. Compared to previously characterized S. suis CPSs (serotypes 1, 1/2, 2 and 14), serotype 9 CPS does not contain sialic acid but contains a labile 4-keto sugar (2-acetamido-2,6-dideoxy-β-D-xylo-hexopyranos-4-ulose), one particular feature of this serotype.
27423880	4	11	theme	4-keto	892:897	arg1	2-acetamido-2,6-dideoxy-β-D-xylo-hexopyranos-4-ulose					906:957	2-acetamido-2,6-dideoxy-β-D-xylo-hexopyranos-4-ulose	906:957	2-acetamido-2,6-dideoxy-β-D-xylo-hexopyranos-4-ulose	906:957	Chemical and spectroscopic data gave the repeating unit sequence: [3)Glcol-6-P-3-[D-Gal(α1-2)]D-Gal(β1-3)D-Sug(β1-3)L-Rha(α1-)]n. Compared to previously characterized S. suis CPSs (serotypes 1, 1/2, 2 and 14), serotype 9 CPS does not contain sialic acid but contains a labile 4-keto sugar (2-acetamido-2,6-dideoxy-β-D-xylo-hexopyranos-4-ulose), one particular feature of this serotype.
27423880	4	12	theme	α1-2	704:707	arg1	α1-					738:740	α1-	738:740	α1-	738:740	Chemical and spectroscopic data gave the repeating unit sequence: [3)Glcol-6-P-3-[D-Gal(α1-2)]D-Gal(β1-3)D-Sug(β1-3)L-Rha(α1-)]n. Compared to previously characterized S. suis CPSs (serotypes 1, 1/2, 2 and 14), serotype 9 CPS does not contain sialic acid but contains a labile 4-keto sugar (2-acetamido-2,6-dideoxy-β-D-xylo-hexopyranos-4-ulose), one particular feature of this serotype.
27423880	4	12	theme	α1-2	704:707	arg1	L-Rha					732:736	[3)Glcol-6-P-3-[D-Gal(α1-2)]D-Gal(β1-3)D-Sug(β1-3)L-Rha	682:736	[3)Glcol-6-P-3-[D-Gal(α1-2)]D-Gal(β1-3)D-Sug(β1-3)L-Rha(α1-)]n.	682:744	Chemical and spectroscopic data gave the repeating unit sequence: [3)Glcol-6-P-3-[D-Gal(α1-2)]D-Gal(β1-3)D-Sug(β1-3)L-Rha(α1-)]n. Compared to previously characterized S. suis CPSs (serotypes 1, 1/2, 2 and 14), serotype 9 CPS does not contain sialic acid but contains a labile 4-keto sugar (2-acetamido-2,6-dideoxy-β-D-xylo-hexopyranos-4-ulose), one particular feature of this serotype.
27423880	0	13	theme	cps	116:118	arg1	genes					126:130	the cps locus genes	112:130	the cps locus genes involved in its biosynthesis	112:159	Structure determination of Streptococcus suis serotype 9 capsular polysaccharide and assignment of functions of the cps locus genes involved in its biosynthesis.
27423880	4	14	contain	contains	874:881	arg1	CPS					837:839	serotype 9 CPS	826:839	serotype 9 CPS	826:839	Chemical and spectroscopic data gave the repeating unit sequence: [3)Glcol-6-P-3-[D-Gal(α1-2)]D-Gal(β1-3)D-Sug(β1-3)L-Rha(α1-)]n. Compared to previously characterized S. suis CPSs (serotypes 1, 1/2, 2 and 14), serotype 9 CPS does not contain sialic acid but contains a labile 4-keto sugar (2-acetamido-2,6-dideoxy-β-D-xylo-hexopyranos-4-ulose), one particular feature of this serotype.
27423880	4	14	contain	contains	874:881	arg2	2-acetamido-2,6-dideoxy-β-D-xylo-hexopyranos-4-ulose					906:957	2-acetamido-2,6-dideoxy-β-D-xylo-hexopyranos-4-ulose	906:957	2-acetamido-2,6-dideoxy-β-D-xylo-hexopyranos-4-ulose	906:957	Chemical and spectroscopic data gave the repeating unit sequence: [3)Glcol-6-P-3-[D-Gal(α1-2)]D-Gal(β1-3)D-Sug(β1-3)L-Rha(α1-)]n. Compared to previously characterized S. suis CPSs (serotypes 1, 1/2, 2 and 14), serotype 9 CPS does not contain sialic acid but contains a labile 4-keto sugar (2-acetamido-2,6-dideoxy-β-D-xylo-hexopyranos-4-ulose), one particular feature of this serotype.
27423880	4	14	contain	contains	874:881	arg2	sugar					899:903	a labile 4-keto sugar	883:903	a labile 4-keto sugar (2-acetamido-2,6-dideoxy-β-D-xylo-hexopyranos-4-ulose)	883:958	Chemical and spectroscopic data gave the repeating unit sequence: [3)Glcol-6-P-3-[D-Gal(α1-2)]D-Gal(β1-3)D-Sug(β1-3)L-Rha(α1-)]n. Compared to previously characterized S. suis CPSs (serotypes 1, 1/2, 2 and 14), serotype 9 CPS does not contain sialic acid but contains a labile 4-keto sugar (2-acetamido-2,6-dideoxy-β-D-xylo-hexopyranos-4-ulose), one particular feature of this serotype.
27423880	4	14	contain	contains	874:881	arg2	feature					976:982	one particular feature	961:982	one particular feature	961:982	Chemical and spectroscopic data gave the repeating unit sequence: [3)Glcol-6-P-3-[D-Gal(α1-2)]D-Gal(β1-3)D-Sug(β1-3)L-Rha(α1-)]n. Compared to previously characterized S. suis CPSs (serotypes 1, 1/2, 2 and 14), serotype 9 CPS does not contain sialic acid but contains a labile 4-keto sugar (2-acetamido-2,6-dideoxy-β-D-xylo-hexopyranos-4-ulose), one particular feature of this serotype.
27423880	3	15	theme	major	511:515	arg1	polysaccharide					487:500	serotype 9 capsular polysaccharide	467:500	serotype 9 capsular polysaccharide (CPS)	467:506	Here we determined for the first time the chemical composition and structure of serotype 9 capsular polysaccharide (CPS), a major bacterial virulence factor and the antigen at the origin of S. suis classification into serotypes.
27423880	3	15	theme	major	511:515	arg1	factor					537:542	a major bacterial virulence factor	509:542	a major bacterial virulence factor	509:542	Here we determined for the first time the chemical composition and structure of serotype 9 capsular polysaccharide (CPS), a major bacterial virulence factor and the antigen at the origin of S. suis classification into serotypes.
27423880	1	16	theme	suis	176:179	arg1	serotype					222:229	the most prevalent S. suis serotype	195:229	the most prevalent S. suis serotype in several European countries	195:259	Streptococcus suis serotype 9 is the most prevalent S. suis serotype in several European countries.
27423880	1	16	theme	suis	176:179	arg1	serotype					181:188	Streptococcus suis serotype 9	162:190	Streptococcus suis serotype 9	162:190	Streptococcus suis serotype 9 is the most prevalent S. suis serotype in several European countries.
27423880	6	17	theme	bacterial	1325:1333	arg1	component					1335:1343	this bacterial component	1320:1343	this bacterial component	1320:1343	Knowledge of CPS structure and composition will contribute to better dissect the role of this bacterial component in the pathogenesis of S. suis serotype 9.
27423880	3	18	theme	bacterial	517:525	arg1	polysaccharide					487:500	serotype 9 capsular polysaccharide	467:500	serotype 9 capsular polysaccharide (CPS)	467:506	Here we determined for the first time the chemical composition and structure of serotype 9 capsular polysaccharide (CPS), a major bacterial virulence factor and the antigen at the origin of S. suis classification into serotypes.
27423880	3	18	theme	bacterial	517:525	arg1	factor					537:542	a major bacterial virulence factor	509:542	a major bacterial virulence factor	509:542	Here we determined for the first time the chemical composition and structure of serotype 9 capsular polysaccharide (CPS), a major bacterial virulence factor and the antigen at the origin of S. suis classification into serotypes.
27423880	6	19	theme	serotype	1376:1383	arg1	pathogenesis					1352:1363	the pathogenesis	1348:1363	the pathogenesis of S. suis serotype 9	1348:1385	Knowledge of CPS structure and composition will contribute to better dissect the role of this bacterial component in the pathogenesis of S. suis serotype 9.
27423880	5	20	theme	locus	1087:1091	arg1	genes					1060:1064	genes	1060:1064	genes of this serotype cps locus encoding putative glycosyltransferases and polymerase responsible for the biosynthesis of the repeating unit	1060:1200	A correlation between S. suis serotype 9 CPS sequence and genes of this serotype cps locus encoding putative glycosyltransferases and polymerase responsible for the biosynthesis of the repeating unit was tentatively established.
27423880	5	20	theme	locus	1087:1091	arg1	sequence					1047:1054	S. suis serotype 9 CPS sequence	1024:1054	S. suis serotype 9 CPS sequence	1024:1054	A correlation between S. suis serotype 9 CPS sequence and genes of this serotype cps locus encoding putative glycosyltransferases and polymerase responsible for the biosynthesis of the repeating unit was tentatively established.
27423880	4	21	theme	S. suis	783:789	arg1	CPSs					791:794	previously characterized S. suis CPSs	758:794	previously characterized S. suis CPSs	758:794	Chemical and spectroscopic data gave the repeating unit sequence: [3)Glcol-6-P-3-[D-Gal(α1-2)]D-Gal(β1-3)D-Sug(β1-3)L-Rha(α1-)]n. Compared to previously characterized S. suis CPSs (serotypes 1, 1/2, 2 and 14), serotype 9 CPS does not contain sialic acid but contains a labile 4-keto sugar (2-acetamido-2,6-dideoxy-β-D-xylo-hexopyranos-4-ulose), one particular feature of this serotype.
27423880	2	22	theme	zoonotic	316:323	arg1	potential					325:333	increasing zoonotic potential	305:333	increasing zoonotic potential	305:333	In spite of its pathogenicity for pigs and increasing zoonotic potential, limited information is available on this serotype.
27423880	4	23	theme	unit	667:670	arg1	sequence					672:679	the repeating unit sequence	653:679	the repeating unit sequence	653:679	Chemical and spectroscopic data gave the repeating unit sequence: [3)Glcol-6-P-3-[D-Gal(α1-2)]D-Gal(β1-3)D-Sug(β1-3)L-Rha(α1-)]n. Compared to previously characterized S. suis CPSs (serotypes 1, 1/2, 2 and 14), serotype 9 CPS does not contain sialic acid but contains a labile 4-keto sugar (2-acetamido-2,6-dideoxy-β-D-xylo-hexopyranos-4-ulose), one particular feature of this serotype.
27423880	0	24	theme	Structure	0:8	arg1	determination					10:22	Structure determination	0:22	Structure determination of Streptococcus suis serotype 9 capsular polysaccharide and assignment of functions of the cps locus genes involved in its biosynthesis.	0:160	Structure determination of Streptococcus suis serotype 9 capsular polysaccharide and assignment of functions of the cps locus genes involved in its biosynthesis.
27423880	4	25	theme	characterized	769:781	arg1	CPSs					791:794	previously characterized S. suis CPSs	758:794	previously characterized S. suis CPSs	758:794	Chemical and spectroscopic data gave the repeating unit sequence: [3)Glcol-6-P-3-[D-Gal(α1-2)]D-Gal(β1-3)D-Sug(β1-3)L-Rha(α1-)]n. Compared to previously characterized S. suis CPSs (serotypes 1, 1/2, 2 and 14), serotype 9 CPS does not contain sialic acid but contains a labile 4-keto sugar (2-acetamido-2,6-dideoxy-β-D-xylo-hexopyranos-4-ulose), one particular feature of this serotype.
27423880	2	26	theme	increasing	305:314	arg1	potential					325:333	increasing zoonotic potential	305:333	increasing zoonotic potential	305:333	In spite of its pathogenicity for pigs and increasing zoonotic potential, limited information is available on this serotype.
27423880	6	27	theme	composition	1262:1272	arg1	Knowledge					1231:1239	Knowledge	1231:1239	Knowledge of CPS structure and composition	1231:1272	Knowledge of CPS structure and composition will contribute to better dissect the role of this bacterial component in the pathogenesis of S. suis serotype 9.
27423880	4	28	theme	repeating	657:665	arg1	sequence					672:679	the repeating unit sequence	653:679	the repeating unit sequence	653:679	Chemical and spectroscopic data gave the repeating unit sequence: [3)Glcol-6-P-3-[D-Gal(α1-2)]D-Gal(β1-3)D-Sug(β1-3)L-Rha(α1-)]n. Compared to previously characterized S. suis CPSs (serotypes 1, 1/2, 2 and 14), serotype 9 CPS does not contain sialic acid but contains a labile 4-keto sugar (2-acetamido-2,6-dideoxy-β-D-xylo-hexopyranos-4-ulose), one particular feature of this serotype.
27423880	0	29	theme	Streptococcus	27:39	arg1	serotype					46:53	Streptococcus suis serotype 9 capsular polysaccharide and assignment	27:94	Streptococcus suis serotype 9 capsular polysaccharide and assignment	27:94	Structure determination of Streptococcus suis serotype 9 capsular polysaccharide and assignment of functions of the cps locus genes involved in its biosynthesis.
27423880	5	30	theme	serotype	1074:1081	arg1	locus					1087:1091	this serotype cps locus	1069:1091	this serotype cps locus	1069:1091	A correlation between S. suis serotype 9 CPS sequence and genes of this serotype cps locus encoding putative glycosyltransferases and polymerase responsible for the biosynthesis of the repeating unit was tentatively established.
27423880	0	31	theme	genes	126:130	arg1	functions					99:107	functions	99:107	functions of the cps locus genes involved in its biosynthesis	99:159	Structure determination of Streptococcus suis serotype 9 capsular polysaccharide and assignment of functions of the cps locus genes involved in its biosynthesis.
27423880	4	32	theme	β1-3	716:719	arg1	α1-					738:740	α1-	738:740	α1-	738:740	Chemical and spectroscopic data gave the repeating unit sequence: [3)Glcol-6-P-3-[D-Gal(α1-2)]D-Gal(β1-3)D-Sug(β1-3)L-Rha(α1-)]n. Compared to previously characterized S. suis CPSs (serotypes 1, 1/2, 2 and 14), serotype 9 CPS does not contain sialic acid but contains a labile 4-keto sugar (2-acetamido-2,6-dideoxy-β-D-xylo-hexopyranos-4-ulose), one particular feature of this serotype.
27423880	4	32	theme	β1-3	716:719	arg1	L-Rha					732:736	[3)Glcol-6-P-3-[D-Gal(α1-2)]D-Gal(β1-3)D-Sug(β1-3)L-Rha	682:736	[3)Glcol-6-P-3-[D-Gal(α1-2)]D-Gal(β1-3)D-Sug(β1-3)L-Rha(α1-)]n.	682:744	Chemical and spectroscopic data gave the repeating unit sequence: [3)Glcol-6-P-3-[D-Gal(α1-2)]D-Gal(β1-3)D-Sug(β1-3)L-Rha(α1-)]n. Compared to previously characterized S. suis CPSs (serotypes 1, 1/2, 2 and 14), serotype 9 CPS does not contain sialic acid but contains a labile 4-keto sugar (2-acetamido-2,6-dideoxy-β-D-xylo-hexopyranos-4-ulose), one particular feature of this serotype.
27423880	3	33	from	origin	567:572	arg1	structure					454:462	structure	454:462	structure	454:462	Here we determined for the first time the chemical composition and structure of serotype 9 capsular polysaccharide (CPS), a major bacterial virulence factor and the antigen at the origin of S. suis classification into serotypes.
27423880	3	33	from	origin	567:572	arg1	composition					438:448	chemical composition	429:448	chemical composition	429:448	Here we determined for the first time the chemical composition and structure of serotype 9 capsular polysaccharide (CPS), a major bacterial virulence factor and the antigen at the origin of S. suis classification into serotypes.
27423880	3	33	from	origin	567:572	arg1	antigen					552:558	the antigen	548:558	the antigen at the origin of S. suis classification into serotypes	548:613	Here we determined for the first time the chemical composition and structure of serotype 9 capsular polysaccharide (CPS), a major bacterial virulence factor and the antigen at the origin of S. suis classification into serotypes.
27423880	4	34	dep	Compared	746:753	arg1	serotypes					797:805	serotypes 1, 1/2, 2 and 14	797:822	serotypes 1, 1/2, 2 and 14	797:822	Chemical and spectroscopic data gave the repeating unit sequence: [3)Glcol-6-P-3-[D-Gal(α1-2)]D-Gal(β1-3)D-Sug(β1-3)L-Rha(α1-)]n. Compared to previously characterized S. suis CPSs (serotypes 1, 1/2, 2 and 14), serotype 9 CPS does not contain sialic acid but contains a labile 4-keto sugar (2-acetamido-2,6-dideoxy-β-D-xylo-hexopyranos-4-ulose), one particular feature of this serotype.
27423880	5	35	theme	cps	1083:1085	arg1	locus					1087:1091	this serotype cps locus	1069:1091	this serotype cps locus	1069:1091	A correlation between S. suis serotype 9 CPS sequence and genes of this serotype cps locus encoding putative glycosyltransferases and polymerase responsible for the biosynthesis of the repeating unit was tentatively established.
27423880	0	36	theme	locus	120:124	arg1	genes					126:130	the cps locus genes	112:130	the cps locus genes involved in its biosynthesis	112:159	Structure determination of Streptococcus suis serotype 9 capsular polysaccharide and assignment of functions of the cps locus genes involved in its biosynthesis.
27423880	3	37	theme	serotype	467:474	arg1	polysaccharide					487:500	serotype 9 capsular polysaccharide	467:500	serotype 9 capsular polysaccharide (CPS)	467:506	Here we determined for the first time the chemical composition and structure of serotype 9 capsular polysaccharide (CPS), a major bacterial virulence factor and the antigen at the origin of S. suis classification into serotypes.
27423880	3	37	theme	serotype	467:474	arg1	factor					537:542	a major bacterial virulence factor	509:542	a major bacterial virulence factor	509:542	Here we determined for the first time the chemical composition and structure of serotype 9 capsular polysaccharide (CPS), a major bacterial virulence factor and the antigen at the origin of S. suis classification into serotypes.
27423880	3	37	theme	serotype	467:474	arg1	CPS					503:505	CPS	503:505	CPS	503:505	Here we determined for the first time the chemical composition and structure of serotype 9 capsular polysaccharide (CPS), a major bacterial virulence factor and the antigen at the origin of S. suis classification into serotypes.
27423880	0	38	theme	serotype	46:53	arg1	determination					10:22	Structure determination	0:22	Structure determination of Streptococcus suis serotype 9 capsular polysaccharide and assignment of functions of the cps locus genes involved in its biosynthesis.	0:160	Structure determination of Streptococcus suis serotype 9 capsular polysaccharide and assignment of functions of the cps locus genes involved in its biosynthesis.
27423880	4	39	theme	Glcol-6-P-3-[D-Gal	685:702	arg1	α1-					738:740	α1-	738:740	α1-	738:740	Chemical and spectroscopic data gave the repeating unit sequence: [3)Glcol-6-P-3-[D-Gal(α1-2)]D-Gal(β1-3)D-Sug(β1-3)L-Rha(α1-)]n. Compared to previously characterized S. suis CPSs (serotypes 1, 1/2, 2 and 14), serotype 9 CPS does not contain sialic acid but contains a labile 4-keto sugar (2-acetamido-2,6-dideoxy-β-D-xylo-hexopyranos-4-ulose), one particular feature of this serotype.
27423880	4	39	theme	Glcol-6-P-3-[D-Gal	685:702	arg1	L-Rha					732:736	[3)Glcol-6-P-3-[D-Gal(α1-2)]D-Gal(β1-3)D-Sug(β1-3)L-Rha	682:736	[3)Glcol-6-P-3-[D-Gal(α1-2)]D-Gal(β1-3)D-Sug(β1-3)L-Rha(α1-)]n.	682:744	Chemical and spectroscopic data gave the repeating unit sequence: [3)Glcol-6-P-3-[D-Gal(α1-2)]D-Gal(β1-3)D-Sug(β1-3)L-Rha(α1-)]n. Compared to previously characterized S. suis CPSs (serotypes 1, 1/2, 2 and 14), serotype 9 CPS does not contain sialic acid but contains a labile 4-keto sugar (2-acetamido-2,6-dideoxy-β-D-xylo-hexopyranos-4-ulose), one particular feature of this serotype.
27423880	4	40	theme	serotype	826:833	arg1	CPS					837:839	serotype 9 CPS	826:839	serotype 9 CPS	826:839	Chemical and spectroscopic data gave the repeating unit sequence: [3)Glcol-6-P-3-[D-Gal(α1-2)]D-Gal(β1-3)D-Sug(β1-3)L-Rha(α1-)]n. Compared to previously characterized S. suis CPSs (serotypes 1, 1/2, 2 and 14), serotype 9 CPS does not contain sialic acid but contains a labile 4-keto sugar (2-acetamido-2,6-dideoxy-β-D-xylo-hexopyranos-4-ulose), one particular feature of this serotype.
27423880	1	41	theme	prevalent	204:212	arg1	serotype					222:229	the most prevalent S. suis serotype	195:229	the most prevalent S. suis serotype in several European countries	195:259	Streptococcus suis serotype 9 is the most prevalent S. suis serotype in several European countries.
27423880	1	41	theme	prevalent	204:212	arg1	serotype					181:188	Streptococcus suis serotype 9	162:190	Streptococcus suis serotype 9	162:190	Streptococcus suis serotype 9 is the most prevalent S. suis serotype in several European countries.
27423880	0	42	theme	suis	41:44	arg1	serotype					46:53	Streptococcus suis serotype 9 capsular polysaccharide and assignment	27:94	Streptococcus suis serotype 9 capsular polysaccharide and assignment	27:94	Structure determination of Streptococcus suis serotype 9 capsular polysaccharide and assignment of functions of the cps locus genes involved in its biosynthesis.
27423880	3	43	theme	first	414:418	arg1	time					420:423	the first time	410:423	the first time	410:423	Here we determined for the first time the chemical composition and structure of serotype 9 capsular polysaccharide (CPS), a major bacterial virulence factor and the antigen at the origin of S. suis classification into serotypes.
27423880	5	44	theme	putative	1102:1109	arg1	glycosyltransferases					1111:1130	putative glycosyltransferases	1102:1130	putative glycosyltransferases	1102:1130	A correlation between S. suis serotype 9 CPS sequence and genes of this serotype cps locus encoding putative glycosyltransferases and polymerase responsible for the biosynthesis of the repeating unit was tentatively established.
27423880	3	45	theme	S. suis	577:583	arg1	classification					585:598	S. suis classification	577:598	S. suis classification into serotypes	577:613	Here we determined for the first time the chemical composition and structure of serotype 9 capsular polysaccharide (CPS), a major bacterial virulence factor and the antigen at the origin of S. suis classification into serotypes.
27423880	1	46	theme	S. suis	214:220	arg1	serotype					222:229	the most prevalent S. suis serotype	195:229	the most prevalent S. suis serotype in several European countries	195:259	Streptococcus suis serotype 9 is the most prevalent S. suis serotype in several European countries.
27423880	1	46	theme	S. suis	214:220	arg1	serotype					181:188	Streptococcus suis serotype 9	162:190	Streptococcus suis serotype 9	162:190	Streptococcus suis serotype 9 is the most prevalent S. suis serotype in several European countries.
27423880	0	47	theme	capsular	57:64	arg1	polysaccharide					66:79	polysaccharide	66:79	polysaccharide	66:79	Structure determination of Streptococcus suis serotype 9 capsular polysaccharide and assignment of functions of the cps locus genes involved in its biosynthesis.
27423880	6	48	theme	CPS	1244:1246	arg1	structure					1248:1256	CPS structure	1244:1256	CPS structure	1244:1256	Knowledge of CPS structure and composition will contribute to better dissect the role of this bacterial component in the pathogenesis of S. suis serotype 9.
27423880	3	49	theme	chemical	429:436	arg1	composition					438:448	chemical composition	429:448	chemical composition	429:448	Here we determined for the first time the chemical composition and structure of serotype 9 capsular polysaccharide (CPS), a major bacterial virulence factor and the antigen at the origin of S. suis classification into serotypes.
27423880	0	50	dep	serotype	46:53	arg1	assignment					85:94	assignment	85:94	assignment	85:94	Structure determination of Streptococcus suis serotype 9 capsular polysaccharide and assignment of functions of the cps locus genes involved in its biosynthesis.
27423880	0	50	dep	serotype	46:53	arg1	polysaccharide					66:79	polysaccharide	66:79	polysaccharide	66:79	Structure determination of Streptococcus suis serotype 9 capsular polysaccharide and assignment of functions of the cps locus genes involved in its biosynthesis.
27423880	6	51	theme	S. suis	1368:1374	arg1	serotype					1376:1383	S. suis serotype 9	1368:1385	S. suis serotype 9	1368:1385	Knowledge of CPS structure and composition will contribute to better dissect the role of this bacterial component in the pathogenesis of S. suis serotype 9.
27423880	3	52	theme	polysaccharide	487:500	arg1	structure					454:462	structure	454:462	structure	454:462	Here we determined for the first time the chemical composition and structure of serotype 9 capsular polysaccharide (CPS), a major bacterial virulence factor and the antigen at the origin of S. suis classification into serotypes.
27423880	3	52	theme	polysaccharide	487:500	arg1	composition					438:448	chemical composition	429:448	chemical composition	429:448	Here we determined for the first time the chemical composition and structure of serotype 9 capsular polysaccharide (CPS), a major bacterial virulence factor and the antigen at the origin of S. suis classification into serotypes.
27423880	3	52	theme	polysaccharide	487:500	arg1	antigen					552:558	the antigen	548:558	the antigen at the origin of S. suis classification into serotypes	548:613	Here we determined for the first time the chemical composition and structure of serotype 9 capsular polysaccharide (CPS), a major bacterial virulence factor and the antigen at the origin of S. suis classification into serotypes.
27423880	6	53	theme	structure	1248:1256	arg1	Knowledge					1231:1239	Knowledge	1231:1239	Knowledge of CPS structure and composition	1231:1272	Knowledge of CPS structure and composition will contribute to better dissect the role of this bacterial component in the pathogenesis of S. suis serotype 9.
27423880	6	54	theme	component	1335:1343	arg1	role					1312:1315	the role	1308:1315	the role of this bacterial component in the pathogenesis of S. suis serotype 9	1308:1385	Knowledge of CPS structure and composition will contribute to better dissect the role of this bacterial component in the pathogenesis of S. suis serotype 9.
27423880	6	55	from	role	1312:1315	arg1	pathogenesis					1352:1363	the pathogenesis	1348:1363	the pathogenesis of S. suis serotype 9	1348:1385	Knowledge of CPS structure and composition will contribute to better dissect the role of this bacterial component in the pathogenesis of S. suis serotype 9.
27423880	3	56	dep	composition	438:448	arg1	the					425:427	the	425:427	the	425:427	Here we determined for the first time the chemical composition and structure of serotype 9 capsular polysaccharide (CPS), a major bacterial virulence factor and the antigen at the origin of S. suis classification into serotypes.
27423880	2	57	theme	limited	336:342	arg1	information					344:354	limited information	336:354	limited information	336:354	In spite of its pathogenicity for pigs and increasing zoonotic potential, limited information is available on this serotype.
27423880	5	58	theme	unit	1197:1200	arg1	biosynthesis					1167:1178	the biosynthesis	1163:1178	the biosynthesis of the repeating unit	1163:1200	A correlation between S. suis serotype 9 CPS sequence and genes of this serotype cps locus encoding putative glycosyltransferases and polymerase responsible for the biosynthesis of the repeating unit was tentatively established.
27423880	1	59	from	serotype	222:229	arg1	countries					251:259	several European countries	234:259	several European countries	234:259	Streptococcus suis serotype 9 is the most prevalent S. suis serotype in several European countries.
27423880	4	60	theme	L-Rha	732:736	arg1	n.					743:744	[3)Glcol-6-P-3-[D-Gal(α1-2)]D-Gal(β1-3)D-Sug(β1-3)L-Rha(α1-)]n.	682:744	[3)Glcol-6-P-3-[D-Gal(α1-2)]D-Gal(β1-3)D-Sug(β1-3)L-Rha(α1-)]n.	682:744	Chemical and spectroscopic data gave the repeating unit sequence: [3)Glcol-6-P-3-[D-Gal(α1-2)]D-Gal(β1-3)D-Sug(β1-3)L-Rha(α1-)]n. Compared to previously characterized S. suis CPSs (serotypes 1, 1/2, 2 and 14), serotype 9 CPS does not contain sialic acid but contains a labile 4-keto sugar (2-acetamido-2,6-dideoxy-β-D-xylo-hexopyranos-4-ulose), one particular feature of this serotype.
27423880	4	61	theme	β1-3	727:730	arg1	α1-					738:740	α1-	738:740	α1-	738:740	Chemical and spectroscopic data gave the repeating unit sequence: [3)Glcol-6-P-3-[D-Gal(α1-2)]D-Gal(β1-3)D-Sug(β1-3)L-Rha(α1-)]n. Compared to previously characterized S. suis CPSs (serotypes 1, 1/2, 2 and 14), serotype 9 CPS does not contain sialic acid but contains a labile 4-keto sugar (2-acetamido-2,6-dideoxy-β-D-xylo-hexopyranos-4-ulose), one particular feature of this serotype.
27423880	4	61	theme	β1-3	727:730	arg1	L-Rha					732:736	[3)Glcol-6-P-3-[D-Gal(α1-2)]D-Gal(β1-3)D-Sug(β1-3)L-Rha	682:736	[3)Glcol-6-P-3-[D-Gal(α1-2)]D-Gal(β1-3)D-Sug(β1-3)L-Rha(α1-)]n.	682:744	Chemical and spectroscopic data gave the repeating unit sequence: [3)Glcol-6-P-3-[D-Gal(α1-2)]D-Gal(β1-3)D-Sug(β1-3)L-Rha(α1-)]n. Compared to previously characterized S. suis CPSs (serotypes 1, 1/2, 2 and 14), serotype 9 CPS does not contain sialic acid but contains a labile 4-keto sugar (2-acetamido-2,6-dideoxy-β-D-xylo-hexopyranos-4-ulose), one particular feature of this serotype.
27423880	4	62	theme	D-Sug	721:725	arg1	α1-					738:740	α1-	738:740	α1-	738:740	Chemical and spectroscopic data gave the repeating unit sequence: [3)Glcol-6-P-3-[D-Gal(α1-2)]D-Gal(β1-3)D-Sug(β1-3)L-Rha(α1-)]n. Compared to previously characterized S. suis CPSs (serotypes 1, 1/2, 2 and 14), serotype 9 CPS does not contain sialic acid but contains a labile 4-keto sugar (2-acetamido-2,6-dideoxy-β-D-xylo-hexopyranos-4-ulose), one particular feature of this serotype.
27423880	4	62	theme	D-Sug	721:725	arg1	L-Rha					732:736	[3)Glcol-6-P-3-[D-Gal(α1-2)]D-Gal(β1-3)D-Sug(β1-3)L-Rha	682:736	[3)Glcol-6-P-3-[D-Gal(α1-2)]D-Gal(β1-3)D-Sug(β1-3)L-Rha(α1-)]n.	682:744	Chemical and spectroscopic data gave the repeating unit sequence: [3)Glcol-6-P-3-[D-Gal(α1-2)]D-Gal(β1-3)D-Sug(β1-3)L-Rha(α1-)]n. Compared to previously characterized S. suis CPSs (serotypes 1, 1/2, 2 and 14), serotype 9 CPS does not contain sialic acid but contains a labile 4-keto sugar (2-acetamido-2,6-dideoxy-β-D-xylo-hexopyranos-4-ulose), one particular feature of this serotype.
27423880	3	63	theme	capsular	478:485	arg1	polysaccharide					487:500	serotype 9 capsular polysaccharide	467:500	serotype 9 capsular polysaccharide (CPS)	467:506	Here we determined for the first time the chemical composition and structure of serotype 9 capsular polysaccharide (CPS), a major bacterial virulence factor and the antigen at the origin of S. suis classification into serotypes.
27423880	3	63	theme	capsular	478:485	arg1	factor					537:542	a major bacterial virulence factor	509:542	a major bacterial virulence factor	509:542	Here we determined for the first time the chemical composition and structure of serotype 9 capsular polysaccharide (CPS), a major bacterial virulence factor and the antigen at the origin of S. suis classification into serotypes.
27423880	3	63	theme	capsular	478:485	arg1	CPS					503:505	CPS	503:505	CPS	503:505	Here we determined for the first time the chemical composition and structure of serotype 9 capsular polysaccharide (CPS), a major bacterial virulence factor and the antigen at the origin of S. suis classification into serotypes.
27423880	4	64	theme	serotype	992:999	arg1	sugar					899:903	a labile 4-keto sugar	883:903	a labile 4-keto sugar (2-acetamido-2,6-dideoxy-β-D-xylo-hexopyranos-4-ulose)	883:958	Chemical and spectroscopic data gave the repeating unit sequence: [3)Glcol-6-P-3-[D-Gal(α1-2)]D-Gal(β1-3)D-Sug(β1-3)L-Rha(α1-)]n. Compared to previously characterized S. suis CPSs (serotypes 1, 1/2, 2 and 14), serotype 9 CPS does not contain sialic acid but contains a labile 4-keto sugar (2-acetamido-2,6-dideoxy-β-D-xylo-hexopyranos-4-ulose), one particular feature of this serotype.
27423880	4	64	theme	serotype	992:999	arg1	feature					976:982	one particular feature	961:982	one particular feature	961:982	Chemical and spectroscopic data gave the repeating unit sequence: [3)Glcol-6-P-3-[D-Gal(α1-2)]D-Gal(β1-3)D-Sug(β1-3)L-Rha(α1-)]n. Compared to previously characterized S. suis CPSs (serotypes 1, 1/2, 2 and 14), serotype 9 CPS does not contain sialic acid but contains a labile 4-keto sugar (2-acetamido-2,6-dideoxy-β-D-xylo-hexopyranos-4-ulose), one particular feature of this serotype.
27423880	4	64	theme	serotype	992:999	arg1	2-acetamido-2,6-dideoxy-β-D-xylo-hexopyranos-4-ulose					906:957	2-acetamido-2,6-dideoxy-β-D-xylo-hexopyranos-4-ulose	906:957	2-acetamido-2,6-dideoxy-β-D-xylo-hexopyranos-4-ulose	906:957	Chemical and spectroscopic data gave the repeating unit sequence: [3)Glcol-6-P-3-[D-Gal(α1-2)]D-Gal(β1-3)D-Sug(β1-3)L-Rha(α1-)]n. Compared to previously characterized S. suis CPSs (serotypes 1, 1/2, 2 and 14), serotype 9 CPS does not contain sialic acid but contains a labile 4-keto sugar (2-acetamido-2,6-dideoxy-β-D-xylo-hexopyranos-4-ulose), one particular feature of this serotype.
27423880	5	65	theme	responsible	1147:1157	arg1	glycosyltransferases					1111:1130	putative glycosyltransferases	1102:1130	putative glycosyltransferases	1102:1130	A correlation between S. suis serotype 9 CPS sequence and genes of this serotype cps locus encoding putative glycosyltransferases and polymerase responsible for the biosynthesis of the repeating unit was tentatively established.
27423880	4	66	theme	spectroscopic	629:641	arg1	data					643:646	Chemical and spectroscopic data	616:646	Chemical and spectroscopic data	616:646	Chemical and spectroscopic data gave the repeating unit sequence: [3)Glcol-6-P-3-[D-Gal(α1-2)]D-Gal(β1-3)D-Sug(β1-3)L-Rha(α1-)]n. Compared to previously characterized S. suis CPSs (serotypes 1, 1/2, 2 and 14), serotype 9 CPS does not contain sialic acid but contains a labile 4-keto sugar (2-acetamido-2,6-dideoxy-β-D-xylo-hexopyranos-4-ulose), one particular feature of this serotype.
27423880	3	67	theme	classification	585:598	arg1	origin					567:572	the origin	563:572	the origin of S. suis classification into serotypes	563:613	Here we determined for the first time the chemical composition and structure of serotype 9 capsular polysaccharide (CPS), a major bacterial virulence factor and the antigen at the origin of S. suis classification into serotypes.
27423880	1	68	theme	several	234:240	arg1	countries					251:259	several European countries	234:259	several European countries	234:259	Streptococcus suis serotype 9 is the most prevalent S. suis serotype in several European countries.
27423880	4	69	theme	labile	885:890	arg1	sugar					899:903	a labile 4-keto sugar	883:903	a labile 4-keto sugar (2-acetamido-2,6-dideoxy-β-D-xylo-hexopyranos-4-ulose)	883:958	Chemical and spectroscopic data gave the repeating unit sequence: [3)Glcol-6-P-3-[D-Gal(α1-2)]D-Gal(β1-3)D-Sug(β1-3)L-Rha(α1-)]n. Compared to previously characterized S. suis CPSs (serotypes 1, 1/2, 2 and 14), serotype 9 CPS does not contain sialic acid but contains a labile 4-keto sugar (2-acetamido-2,6-dideoxy-β-D-xylo-hexopyranos-4-ulose), one particular feature of this serotype.
27423880	4	69	theme	labile	885:890	arg1	feature					976:982	one particular feature	961:982	one particular feature	961:982	Chemical and spectroscopic data gave the repeating unit sequence: [3)Glcol-6-P-3-[D-Gal(α1-2)]D-Gal(β1-3)D-Sug(β1-3)L-Rha(α1-)]n. Compared to previously characterized S. suis CPSs (serotypes 1, 1/2, 2 and 14), serotype 9 CPS does not contain sialic acid but contains a labile 4-keto sugar (2-acetamido-2,6-dideoxy-β-D-xylo-hexopyranos-4-ulose), one particular feature of this serotype.
27423880	4	69	theme	labile	885:890	arg1	2-acetamido-2,6-dideoxy-β-D-xylo-hexopyranos-4-ulose					906:957	2-acetamido-2,6-dideoxy-β-D-xylo-hexopyranos-4-ulose	906:957	2-acetamido-2,6-dideoxy-β-D-xylo-hexopyranos-4-ulose	906:957	Chemical and spectroscopic data gave the repeating unit sequence: [3)Glcol-6-P-3-[D-Gal(α1-2)]D-Gal(β1-3)D-Sug(β1-3)L-Rha(α1-)]n. Compared to previously characterized S. suis CPSs (serotypes 1, 1/2, 2 and 14), serotype 9 CPS does not contain sialic acid but contains a labile 4-keto sugar (2-acetamido-2,6-dideoxy-β-D-xylo-hexopyranos-4-ulose), one particular feature of this serotype.
27423880	5	70	theme	S. suis	1024:1030	arg1	sequence					1047:1054	S. suis serotype 9 CPS sequence	1024:1054	S. suis serotype 9 CPS sequence	1024:1054	A correlation between S. suis serotype 9 CPS sequence and genes of this serotype cps locus encoding putative glycosyltransferases and polymerase responsible for the biosynthesis of the repeating unit was tentatively established.
27423880	1	71	theme	European	242:249	arg1	countries					251:259	several European countries	234:259	several European countries	234:259	Streptococcus suis serotype 9 is the most prevalent S. suis serotype in several European countries.
24299773	7	0	theme	glucuronic	696:705	arg1	content					712:718	glucuronic acid content	696:718	glucuronic acid content	696:718	Also, glucuronic acid content and galactronic acid content of EPS correlated with uronic acid contents of cells.
24299773	8	1	theme	cell	953:956	arg1	surface					958:964	the cell surface	949:964	the cell surface	949:964	Scanning electron microscopy and energy dispersive X-ray spectroscopy analysis confirmed that a considerable amount of metals had precipitated on the cell surface.
24299773	6	2	theme	cells	636:640	arg1	contents					610:617	Uronic acid contents	598:617	Uronic acid contents of metal treated cells	598:640	Uronic acid contents of metal treated cells were higher than control cells of each isolate.
24299773	1	3	theme	metal	150:154	arg1	removal					181:187	metal mixed (chromium+cadmium) removal	150:187	metal mixed (chromium+cadmium) removal	150:187	In the present study, chromium, cadmium and metal mixed (chromium+cadmium) removal and its association with exopolysaccharides and uronic acids production in Synechocystis sp.
24299773	7	4	theme	cells	796:800	arg1	contents					784:791	uronic acid contents	772:791	uronic acid contents of cells	772:800	Also, glucuronic acid content and galactronic acid content of EPS correlated with uronic acid contents of cells.
24299773	9	5	theme	CH	1046:1047	arg1	presence					1034:1041	the presence	1030:1041	the presence	1030:1041	Fourier transform infrared spectrum analysis of EPSs indicated the presence of CH and CO group, which may serve as binding sites for divalent cations.
24299773	9	5	theme	CH	1046:1047	arg1	sites					1090:1094	binding sites	1082:1094	binding sites for divalent cations	1082:1115	Fourier transform infrared spectrum analysis of EPSs indicated the presence of CH and CO group, which may serve as binding sites for divalent cations.
24299773	0	6	theme	monosaccharide	78:91	arg1	composition					93:103	monosaccharide composition	78:103	monosaccharide composition	78:103	Metal removal of cyanobacterial exopolysaccharides by uronic acid content and monosaccharide composition.
24299773	8	7	theme	considerable	899:910	arg1	amount					912:917	a considerable amount	897:917	a considerable amount of metals	897:927	Scanning electron microscopy and energy dispersive X-ray spectroscopy analysis confirmed that a considerable amount of metals had precipitated on the cell surface.
24299773	8	7	theme	considerable	899:910	arg1	metals					922:927	metals	922:927	metals	922:927	Scanning electron microscopy and energy dispersive X-ray spectroscopy analysis confirmed that a considerable amount of metals had precipitated on the cell surface.
24299773	1	8	theme	mixed	156:160	arg1	removal					181:187	metal mixed (chromium+cadmium) removal	150:187	metal mixed (chromium+cadmium) removal	150:187	In the present study, chromium, cadmium and metal mixed (chromium+cadmium) removal and its association with exopolysaccharides and uronic acids production in Synechocystis sp.
24299773	7	9	theme	acid	779:782	arg1	contents					784:791	uronic acid contents	772:791	uronic acid contents of cells	772:800	Also, glucuronic acid content and galactronic acid content of EPS correlated with uronic acid contents of cells.
24299773	3	10	dep	metal	396:400	arg1	metal					419:423	metal	419:423	metal	419:423	It was investigated that BASO671 showed different removal ability when exposed to each metal solely and mixed metal.
24299773	1	11	dep	chromium	128:135	arg1	study					121:125	the present study	109:125	the present study	109:125	In the present study, chromium, cadmium and metal mixed (chromium+cadmium) removal and its association with exopolysaccharides and uronic acids production in Synechocystis sp.
24299773	1	11	dep	chromium	128:135	arg1	production					250:259	production	250:259	production in Synechocystis sp	250:279	In the present study, chromium, cadmium and metal mixed (chromium+cadmium) removal and its association with exopolysaccharides and uronic acids production in Synechocystis sp.
24299773	9	12	theme	spectrum	994:1001	arg1	analysis					1003:1010	spectrum analysis	994:1010	spectrum analysis of EPSs indicated the presence of CH and CO group, which may serve as binding sites for divalent cations	994:1115	Fourier transform infrared spectrum analysis of EPSs indicated the presence of CH and CO group, which may serve as binding sites for divalent cations.
24299773	1	13	theme	Synechocystis	264:276	arg1	sp					278:279	Synechocystis sp	264:279	Synechocystis sp	264:279	In the present study, chromium, cadmium and metal mixed (chromium+cadmium) removal and its association with exopolysaccharides and uronic acids production in Synechocystis sp.
24299773	5	14	theme	metal	581:585	arg1	treatment					587:595	metal treatment	581:595	metal treatment	581:595	Monomer composition of EPS was changed after metal treatment.
24299773	8	15	theme	microscopy	821:830	arg1	analysis					873:880	Scanning electron microscopy and energy dispersive X-ray spectroscopy analysis	803:880	Scanning electron microscopy and energy dispersive X-ray spectroscopy analysis	803:880	Scanning electron microscopy and energy dispersive X-ray spectroscopy analysis confirmed that a considerable amount of metals had precipitated on the cell surface.
24299773	6	16	theme	treated	628:634	arg1	cells					636:640	metal treated cells	622:640	metal treated cells	622:640	Uronic acid contents of metal treated cells were higher than control cells of each isolate.
24299773	3	17	theme	removal	359:365	arg1	ability					367:373	different removal ability	349:373	different removal ability	349:373	It was investigated that BASO671 showed different removal ability when exposed to each metal solely and mixed metal.
24299773	1	18	with	chromium	128:135	arg1	exopolysaccharides					214:231	exopolysaccharides	214:231	exopolysaccharides	214:231	In the present study, chromium, cadmium and metal mixed (chromium+cadmium) removal and its association with exopolysaccharides and uronic acids production in Synechocystis sp.
24299773	1	18	with	chromium	128:135	arg1	acids					244:248	uronic acids	237:248	uronic acids	237:248	In the present study, chromium, cadmium and metal mixed (chromium+cadmium) removal and its association with exopolysaccharides and uronic acids production in Synechocystis sp.
24299773	9	19	theme	divalent	1100:1107	arg1	cations					1109:1115	divalent cations	1100:1115	divalent cations	1100:1115	Fourier transform infrared spectrum analysis of EPSs indicated the presence of CH and CO group, which may serve as binding sites for divalent cations.
24299773	6	20	theme	metal	622:626	arg1	cells					636:640	metal treated cells	622:640	metal treated cells	622:640	Uronic acid contents of metal treated cells were higher than control cells of each isolate.
24299773	9	21	theme	binding	1082:1088	arg1	presence					1034:1041	the presence	1030:1041	the presence	1030:1041	Fourier transform infrared spectrum analysis of EPSs indicated the presence of CH and CO group, which may serve as binding sites for divalent cations.
24299773	9	21	theme	binding	1082:1088	arg1	sites					1090:1094	binding sites	1082:1094	binding sites for divalent cations	1082:1115	Fourier transform infrared spectrum analysis of EPSs indicated the presence of CH and CO group, which may serve as binding sites for divalent cations.
24299773	7	22	theme	EPS	752:754	arg1	content					741:747	galactronic acid content	724:747	galactronic acid content	724:747	Also, glucuronic acid content and galactronic acid content of EPS correlated with uronic acid contents of cells.
24299773	7	22	theme	EPS	752:754	arg1	content					712:718	glucuronic acid content	696:718	glucuronic acid content	696:718	Also, glucuronic acid content and galactronic acid content of EPS correlated with uronic acid contents of cells.
24299773	1	23	theme	chromium+cadmium	163:178	arg1	removal					181:187	metal mixed (chromium+cadmium) removal	150:187	metal mixed (chromium+cadmium) removal	150:187	In the present study, chromium, cadmium and metal mixed (chromium+cadmium) removal and its association with exopolysaccharides and uronic acids production in Synechocystis sp.
24299773	8	24	theme	metals	922:927	arg1	amount					912:917	a considerable amount	897:917	a considerable amount of metals	897:927	Scanning electron microscopy and energy dispersive X-ray spectroscopy analysis confirmed that a considerable amount of metals had precipitated on the cell surface.
24299773	8	24	theme	metals	922:927	arg1	metals					922:927	metals	922:927	metals	922:927	Scanning electron microscopy and energy dispersive X-ray spectroscopy analysis confirmed that a considerable amount of metals had precipitated on the cell surface.
24299773	8	25	theme	electron	812:819	arg1	microscopy					821:830	Scanning electron microscopy	803:830	Scanning electron microscopy	803:830	Scanning electron microscopy and energy dispersive X-ray spectroscopy analysis confirmed that a considerable amount of metals had precipitated on the cell surface.
24299773	0	26	theme	Metal	0:4	arg1	removal					6:12	Metal removal	0:12	Metal removal of cyanobacterial exopolysaccharides by uronic acid content and monosaccharide composition	0:103	Metal removal of cyanobacterial exopolysaccharides by uronic acid content and monosaccharide composition.
24299773	5	27	theme	EPS	559:561	arg1	composition					544:554	Monomer composition	536:554	Monomer composition of EPS	536:561	Monomer composition of EPS was changed after metal treatment.
24299773	5	28	theme	Monomer	536:542	arg1	composition					544:554	Monomer composition	536:554	Monomer composition of EPS	536:561	Monomer composition of EPS was changed after metal treatment.
24299773	6	29	theme	acid	605:608	arg1	contents					610:617	Uronic acid contents	598:617	Uronic acid contents of metal treated cells	598:640	Uronic acid contents of metal treated cells were higher than control cells of each isolate.
24299773	0	30	theme	cyanobacterial	17:30	arg1	exopolysaccharides					32:49	cyanobacterial exopolysaccharides	17:49	cyanobacterial exopolysaccharides	17:49	Metal removal of cyanobacterial exopolysaccharides by uronic acid content and monosaccharide composition.
24299773	1	31	with	association	197:207	arg1	exopolysaccharides					214:231	exopolysaccharides	214:231	exopolysaccharides	214:231	In the present study, chromium, cadmium and metal mixed (chromium+cadmium) removal and its association with exopolysaccharides and uronic acids production in Synechocystis sp.
24299773	1	31	with	association	197:207	arg1	acids					244:248	uronic acids	237:248	uronic acids	237:248	In the present study, chromium, cadmium and metal mixed (chromium+cadmium) removal and its association with exopolysaccharides and uronic acids production in Synechocystis sp.
24299773	6	32	theme	isolate	681:687	arg1	cells					667:671	control cells	659:671	control cells of each isolate	659:687	Uronic acid contents of metal treated cells were higher than control cells of each isolate.
24299773	6	33	theme	Uronic	598:603	arg1	contents					610:617	Uronic acid contents	598:617	Uronic acid contents of metal treated cells	598:640	Uronic acid contents of metal treated cells were higher than control cells of each isolate.
24299773	4	34	theme	Cr	521:522	arg1	+Cd					527:529	Cr(VI)+Cd(II)	521:533	Cr(VI)+Cd(II)	521:533	EPS production by BASO671 was increased following exposure to 15 and 35 ppm Cr(VI), Cd(II) and Cr(VI)+Cd(II).
24299773	9	35	theme	group	1056:1060	arg1	presence					1034:1041	the presence	1030:1041	the presence	1030:1041	Fourier transform infrared spectrum analysis of EPSs indicated the presence of CH and CO group, which may serve as binding sites for divalent cations.
24299773	9	35	theme	group	1056:1060	arg1	sites					1090:1094	binding sites	1082:1094	binding sites for divalent cations	1082:1115	Fourier transform infrared spectrum analysis of EPSs indicated the presence of CH and CO group, which may serve as binding sites for divalent cations.
24299773	4	36	dep	+Cd	527:529	arg1	VI					524:525	VI	524:525	VI	524:525	EPS production by BASO671 was increased following exposure to 15 and 35 ppm Cr(VI), Cd(II) and Cr(VI)+Cd(II).
24299773	1	37	theme	present	113:119	arg1	study					121:125	the present study	109:125	the present study	109:125	In the present study, chromium, cadmium and metal mixed (chromium+cadmium) removal and its association with exopolysaccharides and uronic acids production in Synechocystis sp.
24299773	7	38	theme	galactronic	724:734	arg1	content					741:747	galactronic acid content	724:747	galactronic acid content	724:747	Also, glucuronic acid content and galactronic acid content of EPS correlated with uronic acid contents of cells.
24299773	8	39	theme	Scanning	803:810	arg1	microscopy					821:830	Scanning electron microscopy	803:830	Scanning electron microscopy	803:830	Scanning electron microscopy and energy dispersive X-ray spectroscopy analysis confirmed that a considerable amount of metals had precipitated on the cell surface.
24299773	7	40	theme	acid	707:710	arg1	content					712:718	glucuronic acid content	696:718	glucuronic acid content	696:718	Also, glucuronic acid content and galactronic acid content of EPS correlated with uronic acid contents of cells.
24299773	1	41	from	production	250:259	arg1	sp					278:279	Synechocystis sp	264:279	Synechocystis sp	264:279	In the present study, chromium, cadmium and metal mixed (chromium+cadmium) removal and its association with exopolysaccharides and uronic acids production in Synechocystis sp.
24299773	7	42	theme	acid	736:739	arg1	content					741:747	galactronic acid content	724:747	galactronic acid content	724:747	Also, glucuronic acid content and galactronic acid content of EPS correlated with uronic acid contents of cells.
24299773	0	43	theme	exopolysaccharides	32:49	arg1	removal					6:12	Metal removal	0:12	Metal removal of cyanobacterial exopolysaccharides by uronic acid content and monosaccharide composition	0:103	Metal removal of cyanobacterial exopolysaccharides by uronic acid content and monosaccharide composition.
24299773	8	44	theme	energy	836:841	arg1	spectroscopy					860:871	energy dispersive X-ray spectroscopy	836:871	energy dispersive X-ray spectroscopy	836:871	Scanning electron microscopy and energy dispersive X-ray spectroscopy analysis confirmed that a considerable amount of metals had precipitated on the cell surface.
24299773	0	45	theme	acid	61:64	arg1	content					66:72	uronic acid content	54:72	uronic acid content	54:72	Metal removal of cyanobacterial exopolysaccharides by uronic acid content and monosaccharide composition.
24299773	9	46	theme	CO	1053:1054	arg1	group					1056:1060	CO group	1053:1060	CO group	1053:1060	Fourier transform infrared spectrum analysis of EPSs indicated the presence of CH and CO group, which may serve as binding sites for divalent cations.
24299773	0	47	theme	uronic	54:59	arg1	content					66:72	uronic acid content	54:72	uronic acid content	54:72	Metal removal of cyanobacterial exopolysaccharides by uronic acid content and monosaccharide composition.
24299773	4	48	dep	Cr	502:503	arg1	ppm					498:500	ppm	498:500	ppm	498:500	EPS production by BASO671 was increased following exposure to 15 and 35 ppm Cr(VI), Cd(II) and Cr(VI)+Cd(II).
24299773	4	48	dep	Cr	502:503	arg1	VI					505:506	VI	505:506	VI	505:506	EPS production by BASO671 was increased following exposure to 15 and 35 ppm Cr(VI), Cd(II) and Cr(VI)+Cd(II).
24299773	1	49	with	removal	181:187	arg1	exopolysaccharides					214:231	exopolysaccharides	214:231	exopolysaccharides	214:231	In the present study, chromium, cadmium and metal mixed (chromium+cadmium) removal and its association with exopolysaccharides and uronic acids production in Synechocystis sp.
24299773	1	49	with	removal	181:187	arg1	acids					244:248	uronic acids	237:248	uronic acids	237:248	In the present study, chromium, cadmium and metal mixed (chromium+cadmium) removal and its association with exopolysaccharides and uronic acids production in Synechocystis sp.
24299773	8	50	theme	X-ray	854:858	arg1	spectroscopy					860:871	energy dispersive X-ray spectroscopy	836:871	energy dispersive X-ray spectroscopy	836:871	Scanning electron microscopy and energy dispersive X-ray spectroscopy analysis confirmed that a considerable amount of metals had precipitated on the cell surface.
24299773	1	51	with	cadmium	138:144	arg1	exopolysaccharides					214:231	exopolysaccharides	214:231	exopolysaccharides	214:231	In the present study, chromium, cadmium and metal mixed (chromium+cadmium) removal and its association with exopolysaccharides and uronic acids production in Synechocystis sp.
24299773	1	51	with	cadmium	138:144	arg1	acids					244:248	uronic acids	237:248	uronic acids	237:248	In the present study, chromium, cadmium and metal mixed (chromium+cadmium) removal and its association with exopolysaccharides and uronic acids production in Synechocystis sp.
24299773	4	52	dep	ppm	498:500	arg1	35					495:496	35	495:496	35	495:496	EPS production by BASO671 was increased following exposure to 15 and 35 ppm Cr(VI), Cd(II) and Cr(VI)+Cd(II).
24299773	4	52	dep	ppm	498:500	arg1	15					488:489	15	488:489	15	488:489	EPS production by BASO671 was increased following exposure to 15 and 35 ppm Cr(VI), Cd(II) and Cr(VI)+Cd(II).
24299773	9	53	dep	transform	975:983	arg1	infrared					985:992	infrared	985:992	transform infrared spectrum analysis of EPSs indicated the presence of CH and CO group, which may serve as binding sites for divalent cations	975:1115	Fourier transform infrared spectrum analysis of EPSs indicated the presence of CH and CO group, which may serve as binding sites for divalent cations.
24299773	4	54	theme	EPS	426:428	arg1	production					430:439	EPS production	426:439	EPS production by BASO671	426:450	EPS production by BASO671 was increased following exposure to 15 and 35 ppm Cr(VI), Cd(II) and Cr(VI)+Cd(II).
24299773	6	55	theme	control	659:665	arg1	cells					667:671	control cells	659:671	control cells of each isolate	659:687	Uronic acid contents of metal treated cells were higher than control cells of each isolate.
24299773	8	56	theme	dispersive	843:852	arg1	spectroscopy					860:871	energy dispersive X-ray spectroscopy	836:871	energy dispersive X-ray spectroscopy	836:871	Scanning electron microscopy and energy dispersive X-ray spectroscopy analysis confirmed that a considerable amount of metals had precipitated on the cell surface.
24299773	7	57	theme	uronic	772:777	arg1	contents					784:791	uronic acid contents	772:791	uronic acid contents of cells	772:800	Also, glucuronic acid content and galactronic acid content of EPS correlated with uronic acid contents of cells.
24299773	9	58	theme	EPSs	1015:1018	arg1	analysis					1003:1010	spectrum analysis	994:1010	spectrum analysis of EPSs indicated the presence of CH and CO group, which may serve as binding sites for divalent cations	994:1115	Fourier transform infrared spectrum analysis of EPSs indicated the presence of CH and CO group, which may serve as binding sites for divalent cations.
24299773	8	59	theme	spectroscopy	860:871	arg1	analysis					873:880	Scanning electron microscopy and energy dispersive X-ray spectroscopy analysis	803:880	Scanning electron microscopy and energy dispersive X-ray spectroscopy analysis	803:880	Scanning electron microscopy and energy dispersive X-ray spectroscopy analysis confirmed that a considerable amount of metals had precipitated on the cell surface.
24299773	1	60	theme	uronic	237:242	arg1	acids					244:248	uronic acids	237:248	uronic acids	237:248	In the present study, chromium, cadmium and metal mixed (chromium+cadmium) removal and its association with exopolysaccharides and uronic acids production in Synechocystis sp.
24299773	3	61	theme	different	349:357	arg1	ability					367:373	different removal ability	349:373	different removal ability	349:373	It was investigated that BASO671 showed different removal ability when exposed to each metal solely and mixed metal.
29874447	2	0	theme	Staphylococcus	493:506	arg1	infection					515:523	Staphylococcus aureus infection	493:523	Staphylococcus aureus infection	493:523	The model of thermal burns complicated by Staphylococcus aureus infection was used.
29874447	4	1	theme	highest	734:740	arg1	activity					756:763	the highest wound healing activity	730:763	the highest wound healing activity	730:763	The gel containing sulfated polysaccharides from brown algae showed the highest wound healing activity.
29874447	3	2	theme	coating	653:659	arg1	healing					608:614	pronounced wound healing	591:614	pronounced wound healing	591:614	Planimetric and bacteriological investigations revealed pronounced wound healing and antibacterial effects of the gel coating.
29874447	3	2	theme	coating	653:659	arg1	effects					634:640	antibacterial effects	620:640	antibacterial effects of the gel coating	620:659	Planimetric and bacteriological investigations revealed pronounced wound healing and antibacterial effects of the gel coating.
29874447	4	3	theme	healing	748:754	arg1	activity					756:763	the highest wound healing activity	730:763	the highest wound healing activity	730:763	The gel containing sulfated polysaccharides from brown algae showed the highest wound healing activity.
29874447	1	4	theme	brown	358:362	arg1	algae					364:368	brown algae	358:368	brown algae	358:368	The data on the experimental study of the efficacy of the gel wound dressings based on chitosan and calcium alginate containing bioactive substances from marine hydrobionts with complex therapeutic action (sulfated polysaccharides from brown algae, hydrolyzed bivalves, peptides from nerve ganglia of cephalopods) are described.
29874447	4	5	contain	containing	670:679	arg1	gel					666:668	The gel	662:668	The gel containing sulfated polysaccharides from brown algae	662:721	The gel containing sulfated polysaccharides from brown algae showed the highest wound healing activity.
29874447	4	5	contain	containing	670:679	arg2	polysaccharides					690:704	sulfated polysaccharides	681:704	sulfated polysaccharides from brown algae	681:721	The gel containing sulfated polysaccharides from brown algae showed the highest wound healing activity.
29874447	1	6	theme	bioactive	250:258	arg1	substances					260:269	bioactive substances	250:269	bioactive substances	250:269	The data on the experimental study of the efficacy of the gel wound dressings based on chitosan and calcium alginate containing bioactive substances from marine hydrobionts with complex therapeutic action (sulfated polysaccharides from brown algae, hydrolyzed bivalves, peptides from nerve ganglia of cephalopods) are described.
29874447	0	7	theme	Wound	105:109	arg1	Dressings					111:119	New Wound Dressings	101:119	New Wound Dressings	101:119	Biologically Active Substances from Marine Hydrobionts with Antibacterial Activity in Composition of New Wound Dressings.
29874447	3	8	theme	wound	602:606	arg1	healing					608:614	pronounced wound healing	591:614	pronounced wound healing	591:614	Planimetric and bacteriological investigations revealed pronounced wound healing and antibacterial effects of the gel coating.
29874447	0	9	theme	New	101:103	arg1	Dressings					111:119	New Wound Dressings	101:119	New Wound Dressings	101:119	Biologically Active Substances from Marine Hydrobionts with Antibacterial Activity in Composition of New Wound Dressings.
29874447	3	10	dep	Planimetric	535:545	arg1	investigations					567:580	investigations	567:580	investigations	567:580	Planimetric and bacteriological investigations revealed pronounced wound healing and antibacterial effects of the gel coating.
29874447	4	11	theme	wound	742:746	arg1	healing					748:754	wound healing	742:754	the highest wound healing activity	730:763	The gel containing sulfated polysaccharides from brown algae showed the highest wound healing activity.
29874447	1	12	theme	hydrolyzed	371:380	arg1	polysaccharides					337:351	sulfated polysaccharides	328:351	sulfated polysaccharides from brown algae	328:368	The data on the experimental study of the efficacy of the gel wound dressings based on chitosan and calcium alginate containing bioactive substances from marine hydrobionts with complex therapeutic action (sulfated polysaccharides from brown algae, hydrolyzed bivalves, peptides from nerve ganglia of cephalopods) are described.
29874447	1	12	theme	hydrolyzed	371:380	arg1	bivalves					382:389	hydrolyzed bivalves	371:389	hydrolyzed bivalves	371:389	The data on the experimental study of the efficacy of the gel wound dressings based on chitosan and calcium alginate containing bioactive substances from marine hydrobionts with complex therapeutic action (sulfated polysaccharides from brown algae, hydrolyzed bivalves, peptides from nerve ganglia of cephalopods) are described.
29874447	4	13	from	algae	717:721	arg1	polysaccharides					690:704	sulfated polysaccharides	681:704	sulfated polysaccharides from brown algae	681:721	The gel containing sulfated polysaccharides from brown algae showed the highest wound healing activity.
29874447	1	14	theme	efficacy	164:171	arg1	study					151:155	the experimental study	134:155	the experimental study of the efficacy of the gel wound dressings based on chitosan and calcium alginate containing bioactive substances from marine hydrobionts with complex therapeutic action (sulfated polysaccharides from brown algae, hydrolyzed bivalves, peptides from nerve ganglia of cephalopods)	134:434	The data on the experimental study of the efficacy of the gel wound dressings based on chitosan and calcium alginate containing bioactive substances from marine hydrobionts with complex therapeutic action (sulfated polysaccharides from brown algae, hydrolyzed bivalves, peptides from nerve ganglia of cephalopods) are described.
29874447	0	15	theme	Dressings	111:119	arg1	Composition					86:96	Composition	86:96	Composition of New Wound Dressings	86:119	Biologically Active Substances from Marine Hydrobionts with Antibacterial Activity in Composition of New Wound Dressings.
29874447	0	16	from	Hydrobionts	43:53	arg1	Substances					20:29	Biologically Active Substances	0:29	Biologically Active Substances from Marine Hydrobionts with Antibacterial Activity in Composition of New Wound Dressings.	0:120	Biologically Active Substances from Marine Hydrobionts with Antibacterial Activity in Composition of New Wound Dressings.
29874447	0	17	theme	Active	13:18	arg1	Substances					20:29	Biologically Active Substances	0:29	Biologically Active Substances from Marine Hydrobionts with Antibacterial Activity in Composition of New Wound Dressings.	0:120	Biologically Active Substances from Marine Hydrobionts with Antibacterial Activity in Composition of New Wound Dressings.
29874447	1	18	theme	marine	276:281	arg1	hydrobionts					283:293	marine hydrobionts	276:293	marine hydrobionts with complex therapeutic action	276:325	The data on the experimental study of the efficacy of the gel wound dressings based on chitosan and calcium alginate containing bioactive substances from marine hydrobionts with complex therapeutic action (sulfated polysaccharides from brown algae, hydrolyzed bivalves, peptides from nerve ganglia of cephalopods) are described.
29874447	4	19	theme	brown	711:715	arg1	algae					717:721	brown algae	711:721	brown algae	711:721	The gel containing sulfated polysaccharides from brown algae showed the highest wound healing activity.
29874447	1	20	theme	gel	180:182	arg1	dressings					190:198	the gel wound dressings	176:198	the gel wound dressings based on chitosan and calcium alginate containing bioactive substances from marine hydrobionts with complex therapeutic action (sulfated polysaccharides from brown algae, hydrolyzed bivalves, peptides from nerve ganglia of cephalopods)	176:434	The data on the experimental study of the efficacy of the gel wound dressings based on chitosan and calcium alginate containing bioactive substances from marine hydrobionts with complex therapeutic action (sulfated polysaccharides from brown algae, hydrolyzed bivalves, peptides from nerve ganglia of cephalopods) are described.
29874447	1	20	theme	gel	180:182	arg1	polysaccharides					337:351	sulfated polysaccharides	328:351	sulfated polysaccharides from brown algae	328:368	The data on the experimental study of the efficacy of the gel wound dressings based on chitosan and calcium alginate containing bioactive substances from marine hydrobionts with complex therapeutic action (sulfated polysaccharides from brown algae, hydrolyzed bivalves, peptides from nerve ganglia of cephalopods) are described.
29874447	1	21	from	data	126:129	arg1	study					151:155	the experimental study	134:155	the experimental study of the efficacy of the gel wound dressings based on chitosan and calcium alginate containing bioactive substances from marine hydrobionts with complex therapeutic action (sulfated polysaccharides from brown algae, hydrolyzed bivalves, peptides from nerve ganglia of cephalopods)	134:434	The data on the experimental study of the efficacy of the gel wound dressings based on chitosan and calcium alginate containing bioactive substances from marine hydrobionts with complex therapeutic action (sulfated polysaccharides from brown algae, hydrolyzed bivalves, peptides from nerve ganglia of cephalopods) are described.
29874447	3	22	theme	pronounced	591:600	arg1	healing					608:614	pronounced wound healing	591:614	pronounced wound healing	591:614	Planimetric and bacteriological investigations revealed pronounced wound healing and antibacterial effects of the gel coating.
29874447	1	23	theme	wound	184:188	arg1	dressings					190:198	the gel wound dressings	176:198	the gel wound dressings based on chitosan and calcium alginate containing bioactive substances from marine hydrobionts with complex therapeutic action (sulfated polysaccharides from brown algae, hydrolyzed bivalves, peptides from nerve ganglia of cephalopods)	176:434	The data on the experimental study of the efficacy of the gel wound dressings based on chitosan and calcium alginate containing bioactive substances from marine hydrobionts with complex therapeutic action (sulfated polysaccharides from brown algae, hydrolyzed bivalves, peptides from nerve ganglia of cephalopods) are described.
29874447	1	23	theme	wound	184:188	arg1	polysaccharides					337:351	sulfated polysaccharides	328:351	sulfated polysaccharides from brown algae	328:368	The data on the experimental study of the efficacy of the gel wound dressings based on chitosan and calcium alginate containing bioactive substances from marine hydrobionts with complex therapeutic action (sulfated polysaccharides from brown algae, hydrolyzed bivalves, peptides from nerve ganglia of cephalopods) are described.
29874447	2	24	theme	burns	472:476	arg1	model					455:459	The model	451:459	The model of thermal burns complicated by Staphylococcus aureus infection	451:523	The model of thermal burns complicated by Staphylococcus aureus infection was used.
29874447	2	25	theme	thermal	464:470	arg1	burns					472:476	thermal burns	464:476	thermal burns complicated by Staphylococcus aureus infection	464:523	The model of thermal burns complicated by Staphylococcus aureus infection was used.
29874447	1	26	theme	dressings	190:198	arg1	efficacy					164:171	the efficacy	160:171	the efficacy of the gel wound dressings based on chitosan and calcium alginate containing bioactive substances from marine hydrobionts with complex therapeutic action (sulfated polysaccharides from brown algae, hydrolyzed bivalves, peptides from nerve ganglia of cephalopods)	160:434	The data on the experimental study of the efficacy of the gel wound dressings based on chitosan and calcium alginate containing bioactive substances from marine hydrobionts with complex therapeutic action (sulfated polysaccharides from brown algae, hydrolyzed bivalves, peptides from nerve ganglia of cephalopods) are described.
29874447	1	27	theme	complex	300:306	arg1	action					320:325	complex therapeutic action	300:325	complex therapeutic action	300:325	The data on the experimental study of the efficacy of the gel wound dressings based on chitosan and calcium alginate containing bioactive substances from marine hydrobionts with complex therapeutic action (sulfated polysaccharides from brown algae, hydrolyzed bivalves, peptides from nerve ganglia of cephalopods) are described.
29874447	2	28	used	used	529:532	arg2	model					455:459	The model	451:459	The model of thermal burns complicated by Staphylococcus aureus infection	451:523	The model of thermal burns complicated by Staphylococcus aureus infection was used.
29874447	0	29	theme	Marine	36:41	arg1	Hydrobionts					43:53	Marine Hydrobionts	36:53	Marine Hydrobionts	36:53	Biologically Active Substances from Marine Hydrobionts with Antibacterial Activity in Composition of New Wound Dressings.
29874447	1	30	theme	therapeutic	308:318	arg1	action					320:325	complex therapeutic action	300:325	complex therapeutic action	300:325	The data on the experimental study of the efficacy of the gel wound dressings based on chitosan and calcium alginate containing bioactive substances from marine hydrobionts with complex therapeutic action (sulfated polysaccharides from brown algae, hydrolyzed bivalves, peptides from nerve ganglia of cephalopods) are described.
29874447	1	31	from	algae	364:368	arg1	dressings					190:198	the gel wound dressings	176:198	the gel wound dressings based on chitosan and calcium alginate containing bioactive substances from marine hydrobionts with complex therapeutic action (sulfated polysaccharides from brown algae, hydrolyzed bivalves, peptides from nerve ganglia of cephalopods)	176:434	The data on the experimental study of the efficacy of the gel wound dressings based on chitosan and calcium alginate containing bioactive substances from marine hydrobionts with complex therapeutic action (sulfated polysaccharides from brown algae, hydrolyzed bivalves, peptides from nerve ganglia of cephalopods) are described.
29874447	1	31	from	algae	364:368	arg1	polysaccharides					337:351	sulfated polysaccharides	328:351	sulfated polysaccharides from brown algae	328:368	The data on the experimental study of the efficacy of the gel wound dressings based on chitosan and calcium alginate containing bioactive substances from marine hydrobionts with complex therapeutic action (sulfated polysaccharides from brown algae, hydrolyzed bivalves, peptides from nerve ganglia of cephalopods) are described.
29874447	1	31	from	algae	364:368	arg1	bivalves					382:389	hydrolyzed bivalves	371:389	hydrolyzed bivalves	371:389	The data on the experimental study of the efficacy of the gel wound dressings based on chitosan and calcium alginate containing bioactive substances from marine hydrobionts with complex therapeutic action (sulfated polysaccharides from brown algae, hydrolyzed bivalves, peptides from nerve ganglia of cephalopods) are described.
29874447	1	31	from	algae	364:368	arg1	peptides					392:399	peptides	392:399	peptides from nerve ganglia of cephalopods	392:433	The data on the experimental study of the efficacy of the gel wound dressings based on chitosan and calcium alginate containing bioactive substances from marine hydrobionts with complex therapeutic action (sulfated polysaccharides from brown algae, hydrolyzed bivalves, peptides from nerve ganglia of cephalopods) are described.
29874447	1	32	theme	nerve	406:410	arg1	ganglia					412:418	nerve ganglia	406:418	nerve ganglia of cephalopods	406:433	The data on the experimental study of the efficacy of the gel wound dressings based on chitosan and calcium alginate containing bioactive substances from marine hydrobionts with complex therapeutic action (sulfated polysaccharides from brown algae, hydrolyzed bivalves, peptides from nerve ganglia of cephalopods) are described.
29874447	1	33	with	hydrobionts	283:293	arg1	action					320:325	complex therapeutic action	300:325	complex therapeutic action	300:325	The data on the experimental study of the efficacy of the gel wound dressings based on chitosan and calcium alginate containing bioactive substances from marine hydrobionts with complex therapeutic action (sulfated polysaccharides from brown algae, hydrolyzed bivalves, peptides from nerve ganglia of cephalopods) are described.
29874447	4	34	theme	sulfated	681:688	arg1	polysaccharides					690:704	sulfated polysaccharides	681:704	sulfated polysaccharides from brown algae	681:721	The gel containing sulfated polysaccharides from brown algae showed the highest wound healing activity.
29874447	0	35	theme	Antibacterial	60:72	arg1	Activity					74:81	Antibacterial Activity	60:81	Antibacterial Activity in Composition of New Wound Dressings	60:119	Biologically Active Substances from Marine Hydrobionts with Antibacterial Activity in Composition of New Wound Dressings.
29874447	0	36	from	Activity	74:81	arg1	Composition					86:96	Composition	86:96	Composition of New Wound Dressings	86:119	Biologically Active Substances from Marine Hydrobionts with Antibacterial Activity in Composition of New Wound Dressings.
29874447	3	37	theme	antibacterial	620:632	arg1	effects					634:640	antibacterial effects	620:640	antibacterial effects of the gel coating	620:659	Planimetric and bacteriological investigations revealed pronounced wound healing and antibacterial effects of the gel coating.
29874447	0	38	with	Substances	20:29	arg1	Activity					74:81	Antibacterial Activity	60:81	Antibacterial Activity in Composition of New Wound Dressings	60:119	Biologically Active Substances from Marine Hydrobionts with Antibacterial Activity in Composition of New Wound Dressings.
29874447	1	39	theme	sulfated	328:335	arg1	dressings					190:198	the gel wound dressings	176:198	the gel wound dressings based on chitosan and calcium alginate containing bioactive substances from marine hydrobionts with complex therapeutic action (sulfated polysaccharides from brown algae, hydrolyzed bivalves, peptides from nerve ganglia of cephalopods)	176:434	The data on the experimental study of the efficacy of the gel wound dressings based on chitosan and calcium alginate containing bioactive substances from marine hydrobionts with complex therapeutic action (sulfated polysaccharides from brown algae, hydrolyzed bivalves, peptides from nerve ganglia of cephalopods) are described.
29874447	1	39	theme	sulfated	328:335	arg1	polysaccharides					337:351	sulfated polysaccharides	328:351	sulfated polysaccharides from brown algae	328:368	The data on the experimental study of the efficacy of the gel wound dressings based on chitosan and calcium alginate containing bioactive substances from marine hydrobionts with complex therapeutic action (sulfated polysaccharides from brown algae, hydrolyzed bivalves, peptides from nerve ganglia of cephalopods) are described.
29874447	1	39	theme	sulfated	328:335	arg1	bivalves					382:389	hydrolyzed bivalves	371:389	hydrolyzed bivalves	371:389	The data on the experimental study of the efficacy of the gel wound dressings based on chitosan and calcium alginate containing bioactive substances from marine hydrobionts with complex therapeutic action (sulfated polysaccharides from brown algae, hydrolyzed bivalves, peptides from nerve ganglia of cephalopods) are described.
29874447	1	39	theme	sulfated	328:335	arg1	peptides					392:399	peptides	392:399	peptides from nerve ganglia of cephalopods	392:433	The data on the experimental study of the efficacy of the gel wound dressings based on chitosan and calcium alginate containing bioactive substances from marine hydrobionts with complex therapeutic action (sulfated polysaccharides from brown algae, hydrolyzed bivalves, peptides from nerve ganglia of cephalopods) are described.
29874447	1	40	theme	cephalopods	423:433	arg1	ganglia					412:418	nerve ganglia	406:418	nerve ganglia of cephalopods	406:433	The data on the experimental study of the efficacy of the gel wound dressings based on chitosan and calcium alginate containing bioactive substances from marine hydrobionts with complex therapeutic action (sulfated polysaccharides from brown algae, hydrolyzed bivalves, peptides from nerve ganglia of cephalopods) are described.
29874447	1	41	dep	based	200:204	arg1	dressings					190:198	the gel wound dressings	176:198	the gel wound dressings based on chitosan and calcium alginate containing bioactive substances from marine hydrobionts with complex therapeutic action (sulfated polysaccharides from brown algae, hydrolyzed bivalves, peptides from nerve ganglia of cephalopods)	176:434	The data on the experimental study of the efficacy of the gel wound dressings based on chitosan and calcium alginate containing bioactive substances from marine hydrobionts with complex therapeutic action (sulfated polysaccharides from brown algae, hydrolyzed bivalves, peptides from nerve ganglia of cephalopods) are described.
29874447	1	41	dep	based	200:204	arg1	polysaccharides					337:351	sulfated polysaccharides	328:351	sulfated polysaccharides from brown algae	328:368	The data on the experimental study of the efficacy of the gel wound dressings based on chitosan and calcium alginate containing bioactive substances from marine hydrobionts with complex therapeutic action (sulfated polysaccharides from brown algae, hydrolyzed bivalves, peptides from nerve ganglia of cephalopods) are described.
29874447	1	41	dep	based	200:204	arg1	bivalves					382:389	hydrolyzed bivalves	371:389	hydrolyzed bivalves	371:389	The data on the experimental study of the efficacy of the gel wound dressings based on chitosan and calcium alginate containing bioactive substances from marine hydrobionts with complex therapeutic action (sulfated polysaccharides from brown algae, hydrolyzed bivalves, peptides from nerve ganglia of cephalopods) are described.
29874447	1	41	dep	based	200:204	arg1	peptides					392:399	peptides	392:399	peptides from nerve ganglia of cephalopods	392:433	The data on the experimental study of the efficacy of the gel wound dressings based on chitosan and calcium alginate containing bioactive substances from marine hydrobionts with complex therapeutic action (sulfated polysaccharides from brown algae, hydrolyzed bivalves, peptides from nerve ganglia of cephalopods) are described.
29874447	3	42	theme	gel	649:651	arg1	coating					653:659	the gel coating	645:659	the gel coating	645:659	Planimetric and bacteriological investigations revealed pronounced wound healing and antibacterial effects of the gel coating.
29874447	1	43	theme	experimental	138:149	arg1	study					151:155	the experimental study	134:155	the experimental study of the efficacy of the gel wound dressings based on chitosan and calcium alginate containing bioactive substances from marine hydrobionts with complex therapeutic action (sulfated polysaccharides from brown algae, hydrolyzed bivalves, peptides from nerve ganglia of cephalopods)	134:434	The data on the experimental study of the efficacy of the gel wound dressings based on chitosan and calcium alginate containing bioactive substances from marine hydrobionts with complex therapeutic action (sulfated polysaccharides from brown algae, hydrolyzed bivalves, peptides from nerve ganglia of cephalopods) are described.
29874447	1	44	theme	calcium	222:228	arg1	alginate					230:237	calcium alginate	222:237	calcium alginate	222:237	The data on the experimental study of the efficacy of the gel wound dressings based on chitosan and calcium alginate containing bioactive substances from marine hydrobionts with complex therapeutic action (sulfated polysaccharides from brown algae, hydrolyzed bivalves, peptides from nerve ganglia of cephalopods) are described.
29874447	2	45	theme	aureus	508:513	arg1	infection					515:523	Staphylococcus aureus infection	493:523	Staphylococcus aureus infection	493:523	The model of thermal burns complicated by Staphylococcus aureus infection was used.
29874447	1	46	contain	containing	239:248	arg1	chitosan					209:216	chitosan	209:216	chitosan	209:216	The data on the experimental study of the efficacy of the gel wound dressings based on chitosan and calcium alginate containing bioactive substances from marine hydrobionts with complex therapeutic action (sulfated polysaccharides from brown algae, hydrolyzed bivalves, peptides from nerve ganglia of cephalopods) are described.
29874447	1	46	contain	containing	239:248	arg2	substances					260:269	bioactive substances	250:269	bioactive substances	250:269	The data on the experimental study of the efficacy of the gel wound dressings based on chitosan and calcium alginate containing bioactive substances from marine hydrobionts with complex therapeutic action (sulfated polysaccharides from brown algae, hydrolyzed bivalves, peptides from nerve ganglia of cephalopods) are described.
29874447	1	47	from	ganglia	412:418	arg1	peptides					392:399	peptides	392:399	peptides from nerve ganglia of cephalopods	392:433	The data on the experimental study of the efficacy of the gel wound dressings based on chitosan and calcium alginate containing bioactive substances from marine hydrobionts with complex therapeutic action (sulfated polysaccharides from brown algae, hydrolyzed bivalves, peptides from nerve ganglia of cephalopods) are described.
29874447	1	47	from	ganglia	412:418	arg1	polysaccharides					337:351	sulfated polysaccharides	328:351	sulfated polysaccharides from brown algae	328:368	The data on the experimental study of the efficacy of the gel wound dressings based on chitosan and calcium alginate containing bioactive substances from marine hydrobionts with complex therapeutic action (sulfated polysaccharides from brown algae, hydrolyzed bivalves, peptides from nerve ganglia of cephalopods) are described.
25035429	8	0	theme	sequential	1019:1028	arg1	activity					1030:1037	The sequential activity	1015:1037	The sequential activity of the two enzymes	1015:1056	The sequential activity of the two enzymes leads to the formation of UDP-l-RhaNAc.
25035429	2	1	from	glycans	300:306	arg1	fibrils					311:317	fibrils	311:317	fibrils	311:317	The glycans on fibrils are produced by virally encoded enzymes, organized in gene clusters.
25035429	0	2	theme	acetamido	78:86	arg1	sugars					88:93	uncommon acetamido sugars	69:93	uncommon acetamido sugars	69:93	Giant virus Megavirus chilensis encodes the biosynthetic pathway for uncommon acetamido sugars.
25035429	5	3	from	studies	754:760	arg1	enzymes					700:706	two enzymes	696:706	two enzymes of the pathway with mass spectrometry	696:744	We combined activity assays on two enzymes of the pathway with mass spectrometry and NMR studies to characterize their specificities.
25035429	9	4	theme	glycan	1162:1167	arg1	enzymes					1185:1191	their glycan synthesis using enzymes	1156:1191	their glycan synthesis using enzymes different from their cellular counterparts	1156:1234	This study is another example of giant viruses performing their glycan synthesis using enzymes different from their cellular counterparts, raising again the question of the origin of these pathways.
25035429	1	5	theme	particles	152:160	arg1	fibrils					191:197	the heavily glycosylated fibrils	166:197	the heavily glycosylated fibrils surrounding their capsids	166:223	Giant viruses mimicking microbes, by the sizes of their particles and the heavily glycosylated fibrils surrounding their capsids, infect Acanthamoeba sp., which are ubiquitous unicellular eukaryotes.
25035429	1	5	theme	particles	152:160	arg1	sizes					137:141	the sizes	133:141	the sizes of their particles	133:160	Giant viruses mimicking microbes, by the sizes of their particles and the heavily glycosylated fibrils surrounding their capsids, infect Acanthamoeba sp., which are ubiquitous unicellular eukaryotes.
25035429	9	6	theme	pathways	1287:1294	arg1	origin					1271:1276	the origin	1267:1276	the origin of these pathways	1267:1294	This study is another example of giant viruses performing their glycan synthesis using enzymes different from their cellular counterparts, raising again the question of the origin of these pathways.
25035429	1	7	gly	glycosylated	178:189	arg1	fibrils					191:197	the heavily glycosylated fibrils	166:197	the heavily glycosylated fibrils surrounding their capsids	166:223	Giant viruses mimicking microbes, by the sizes of their particles and the heavily glycosylated fibrils surrounding their capsids, infect Acanthamoeba sp., which are ubiquitous unicellular eukaryotes.
25035429	9	8	theme	synthesis	1169:1177	arg1	enzymes					1185:1191	their glycan synthesis using enzymes	1156:1191	their glycan synthesis using enzymes different from their cellular counterparts	1156:1234	This study is another example of giant viruses performing their glycan synthesis using enzymes different from their cellular counterparts, raising again the question of the origin of these pathways.
25035429	5	9	from	assays	686:691	arg1	enzymes					700:706	two enzymes	696:706	two enzymes of the pathway with mass spectrometry	696:744	We combined activity assays on two enzymes of the pathway with mass spectrometry and NMR studies to characterize their specificities.
25035429	9	10	theme	using	1179:1183	arg1	enzymes					1185:1191	their glycan synthesis using enzymes	1156:1191	their glycan synthesis using enzymes different from their cellular counterparts	1156:1234	This study is another example of giant viruses performing their glycan synthesis using enzymes different from their cellular counterparts, raising again the question of the origin of these pathways.
25035429	1	11	theme	ubiquitous	261:270	arg1	sp.					246:248	Acanthamoeba sp.	233:248	Acanthamoeba sp.	233:248	Giant viruses mimicking microbes, by the sizes of their particles and the heavily glycosylated fibrils surrounding their capsids, infect Acanthamoeba sp., which are ubiquitous unicellular eukaryotes.
25035429	1	11	theme	ubiquitous	261:270	arg1	eukaryotes					284:293	ubiquitous unicellular eukaryotes	261:293	ubiquitous unicellular eukaryotes	261:293	Giant viruses mimicking microbes, by the sizes of their particles and the heavily glycosylated fibrils surrounding their capsids, infect Acanthamoeba sp., which are ubiquitous unicellular eukaryotes.
25035429	5	12	theme	pathway	715:721	arg1	enzymes					700:706	two enzymes	696:706	two enzymes of the pathway with mass spectrometry	696:744	We combined activity assays on two enzymes of the pathway with mass spectrometry and NMR studies to characterize their specificities.
25035429	4	13	theme	Megavirus	638:646	arg1	relatives					654:662	Megavirus close relatives	638:662	Megavirus close relatives	638:662	They also contain N-acetylrhamnosamine (RhaNAc), a rare sugar; the enzymes involved in its synthesis are encoded by a gene cluster specific to Megavirus close relatives.
25035429	9	14	theme	different	1193:1201	arg1	enzymes					1185:1191	their glycan synthesis using enzymes	1156:1191	their glycan synthesis using enzymes different from their cellular counterparts	1156:1234	This study is another example of giant viruses performing their glycan synthesis using enzymes different from their cellular counterparts, raising again the question of the origin of these pathways.
25035429	9	15	from	counterparts	1223:1234	arg1	different					1193:1201	different	1193:1201	different	1193:1201	This study is another example of giant viruses performing their glycan synthesis using enzymes different from their cellular counterparts, raising again the question of the origin of these pathways.
25035429	0	16	theme	virus	6:10	arg1	chilensis					22:30	Giant virus Megavirus chilensis	0:30	Giant virus Megavirus chilensis	0:30	Giant virus Megavirus chilensis encodes the biosynthetic pathway for uncommon acetamido sugars.
25035429	0	16	theme	virus	6:10	arg1	pathway					57:63	the biosynthetic pathway	40:63	the biosynthetic pathway for uncommon acetamido sugars	40:93	Giant virus Megavirus chilensis encodes the biosynthetic pathway for uncommon acetamido sugars.
25035429	5	17	theme	NMR	750:752	arg1	studies					754:760	NMR studies	750:760	NMR studies	750:760	We combined activity assays on two enzymes of the pathway with mass spectrometry and NMR studies to characterize their specificities.
25035429	5	18	theme	mass	728:731	arg1	spectrometry					733:744	mass spectrometry	728:744	mass spectrometry	728:744	We combined activity assays on two enzymes of the pathway with mass spectrometry and NMR studies to characterize their specificities.
25035429	0	19	theme	Giant	0:4	arg1	chilensis					22:30	Giant virus Megavirus chilensis	0:30	Giant virus Megavirus chilensis	0:30	Giant virus Megavirus chilensis encodes the biosynthetic pathway for uncommon acetamido sugars.
25035429	0	19	theme	Giant	0:4	arg1	pathway					57:63	the biosynthetic pathway	40:63	the biosynthetic pathway for uncommon acetamido sugars	40:93	Giant virus Megavirus chilensis encodes the biosynthetic pathway for uncommon acetamido sugars.
25035429	1	20	theme	unicellular	272:282	arg1	sp.					246:248	Acanthamoeba sp.	233:248	Acanthamoeba sp.	233:248	Giant viruses mimicking microbes, by the sizes of their particles and the heavily glycosylated fibrils surrounding their capsids, infect Acanthamoeba sp., which are ubiquitous unicellular eukaryotes.
25035429	1	20	theme	unicellular	272:282	arg1	eukaryotes					284:293	ubiquitous unicellular eukaryotes	261:293	ubiquitous unicellular eukaryotes	261:293	Giant viruses mimicking microbes, by the sizes of their particles and the heavily glycosylated fibrils surrounding their capsids, infect Acanthamoeba sp., which are ubiquitous unicellular eukaryotes.
25035429	8	21	theme	enzymes	1050:1056	arg1	activity					1030:1037	The sequential activity	1015:1037	The sequential activity of the two enzymes	1015:1056	The sequential activity of the two enzymes leads to the formation of UDP-l-RhaNAc.
25035429	1	22	theme	Giant	96:100	arg1	viruses					102:108	Giant viruses	96:108	Giant viruses mimicking microbes	96:127	Giant viruses mimicking microbes, by the sizes of their particles and the heavily glycosylated fibrils surrounding their capsids, infect Acanthamoeba sp., which are ubiquitous unicellular eukaryotes.
25035429	2	23	theme	gene	373:376	arg1	clusters					378:385	gene clusters	373:385	gene clusters	373:385	The glycans on fibrils are produced by virally encoded enzymes, organized in gene clusters.
25035429	4	24	theme	rare	546:549	arg1	sugar					551:555	a rare sugar	544:555	a rare sugar	544:555	They also contain N-acetylrhamnosamine (RhaNAc), a rare sugar; the enzymes involved in its synthesis are encoded by a gene cluster specific to Megavirus close relatives.
25035429	4	24	theme	rare	546:549	arg1	N-acetylrhamnosamine					513:532	N-acetylrhamnosamine	513:532	N-acetylrhamnosamine (RhaNAc)	513:541	They also contain N-acetylrhamnosamine (RhaNAc), a rare sugar; the enzymes involved in its synthesis are encoded by a gene cluster specific to Megavirus close relatives.
25035429	9	25	theme	cellular	1214:1221	arg1	counterparts					1223:1234	their cellular counterparts	1208:1234	their cellular counterparts	1208:1234	This study is another example of giant viruses performing their glycan synthesis using enzymes different from their cellular counterparts, raising again the question of the origin of these pathways.
25035429	4	26	theme	gene	613:616	arg1	cluster					618:624	a gene cluster	611:624	a gene cluster specific to Megavirus close relatives	611:662	They also contain N-acetylrhamnosamine (RhaNAc), a rare sugar; the enzymes involved in its synthesis are encoded by a gene cluster specific to Megavirus close relatives.
25035429	0	27	theme	Megavirus	12:20	arg1	chilensis					22:30	Giant virus Megavirus chilensis	0:30	Giant virus Megavirus chilensis	0:30	Giant virus Megavirus chilensis encodes the biosynthetic pathway for uncommon acetamido sugars.
25035429	0	27	theme	Megavirus	12:20	arg1	pathway					57:63	the biosynthetic pathway	40:63	the biosynthetic pathway for uncommon acetamido sugars	40:93	Giant virus Megavirus chilensis encodes the biosynthetic pathway for uncommon acetamido sugars.
25035429	4	28	contain	contain	505:511	arg2	RhaNAc					535:540	RhaNAc	535:540	RhaNAc	535:540	They also contain N-acetylrhamnosamine (RhaNAc), a rare sugar; the enzymes involved in its synthesis are encoded by a gene cluster specific to Megavirus close relatives.
25035429	4	28	contain	contain	505:511	arg2	sugar					551:555	a rare sugar	544:555	a rare sugar	544:555	They also contain N-acetylrhamnosamine (RhaNAc), a rare sugar; the enzymes involved in its synthesis are encoded by a gene cluster specific to Megavirus close relatives.
25035429	4	28	contain	contain	505:511	arg1	They					495:498	They	495:498	They	495:498	They also contain N-acetylrhamnosamine (RhaNAc), a rare sugar; the enzymes involved in its synthesis are encoded by a gene cluster specific to Megavirus close relatives.
25035429	4	28	contain	contain	505:511	arg2	N-acetylrhamnosamine					513:532	N-acetylrhamnosamine	513:532	N-acetylrhamnosamine (RhaNAc)	513:541	They also contain N-acetylrhamnosamine (RhaNAc), a rare sugar; the enzymes involved in its synthesis are encoded by a gene cluster specific to Megavirus close relatives.
25035429	5	29	with	enzymes	700:706	arg1	spectrometry					733:744	mass spectrometry	728:744	mass spectrometry	728:744	We combined activity assays on two enzymes of the pathway with mass spectrometry and NMR studies to characterize their specificities.
25035429	3	30	theme	Megavirus	404:412	arg1	glycans					414:420	Megavirus glycans	404:420	Megavirus glycans	404:420	Like Mimivirus, Megavirus glycans are mainly composed of virally synthesized N-acetylglucosamine (GlcNAc).
25035429	1	31	theme	glycosylated	178:189	arg1	fibrils					191:197	the heavily glycosylated fibrils	166:197	the heavily glycosylated fibrils surrounding their capsids	166:223	Giant viruses mimicking microbes, by the sizes of their particles and the heavily glycosylated fibrils surrounding their capsids, infect Acanthamoeba sp., which are ubiquitous unicellular eukaryotes.
25035429	4	32	theme	specific	626:633	arg1	cluster					618:624	a gene cluster	611:624	a gene cluster specific to Megavirus close relatives	611:662	They also contain N-acetylrhamnosamine (RhaNAc), a rare sugar; the enzymes involved in its synthesis are encoded by a gene cluster specific to Megavirus close relatives.
25035429	6	33	theme	three-dimensional	843:859	arg1	structure					861:869	its three-dimensional structure	839:869	its three-dimensional structure	839:869	Mg534 is a 4,6-dehydratase 5-epimerase; its three-dimensional structure suggests that it belongs to a third subfamily of inverting dehydratases.
25035429	3	34	theme	synthesized	453:463	arg1	GlcNAc					486:491	GlcNAc	486:491	GlcNAc	486:491	Like Mimivirus, Megavirus glycans are mainly composed of virally synthesized N-acetylglucosamine (GlcNAc).
25035429	3	34	theme	synthesized	453:463	arg1	N-acetylglucosamine					465:483	virally synthesized N-acetylglucosamine	445:483	virally synthesized N-acetylglucosamine (GlcNAc)	445:492	Like Mimivirus, Megavirus glycans are mainly composed of virally synthesized N-acetylglucosamine (GlcNAc).
25035429	2	35	theme	encoded	343:349	arg1	enzymes					351:357	virally encoded enzymes	335:357	virally encoded enzymes	335:357	The glycans on fibrils are produced by virally encoded enzymes, organized in gene clusters.
25035429	0	36	theme	biosynthetic	44:55	arg1	chilensis					22:30	Giant virus Megavirus chilensis	0:30	Giant virus Megavirus chilensis	0:30	Giant virus Megavirus chilensis encodes the biosynthetic pathway for uncommon acetamido sugars.
25035429	0	36	theme	biosynthetic	44:55	arg1	pathway					57:63	the biosynthetic pathway	40:63	the biosynthetic pathway for uncommon acetamido sugars	40:93	Giant virus Megavirus chilensis encodes the biosynthetic pathway for uncommon acetamido sugars.
25035429	6	37	theme	dehydratases	930:941	arg1	subfamily					907:915	a third subfamily	899:915	a third subfamily of inverting dehydratases	899:941	Mg534 is a 4,6-dehydratase 5-epimerase; its three-dimensional structure suggests that it belongs to a third subfamily of inverting dehydratases.
25035429	8	38	theme	UDP-l-RhaNAc	1084:1095	arg1	formation					1071:1079	the formation	1067:1079	the formation of UDP-l-RhaNAc	1067:1095	The sequential activity of the two enzymes leads to the formation of UDP-l-RhaNAc.
25035429	6	39	theme	third	901:905	arg1	subfamily					907:915	a third subfamily	899:915	a third subfamily of inverting dehydratases	899:941	Mg534 is a 4,6-dehydratase 5-epimerase; its three-dimensional structure suggests that it belongs to a third subfamily of inverting dehydratases.
25035429	7	40	theme	bifunctional	977:988	arg1	Mg535					944:948	Mg535	944:948	Mg535	944:948	Mg535, next in the pathway, is a bifunctional 3-epimerase 4-reductase.
25035429	7	40	theme	bifunctional	977:988	arg1	4-reductase					1002:1012	a bifunctional 3-epimerase 4-reductase	975:1012	a bifunctional 3-epimerase 4-reductase	975:1012	Mg535, next in the pathway, is a bifunctional 3-epimerase 4-reductase.
25035429	4	41	theme	close	648:652	arg1	relatives					654:662	Megavirus close relatives	638:662	Megavirus close relatives	638:662	They also contain N-acetylrhamnosamine (RhaNAc), a rare sugar; the enzymes involved in its synthesis are encoded by a gene cluster specific to Megavirus close relatives.
25035429	6	42	theme	inverting	920:928	arg1	dehydratases					930:941	inverting dehydratases	920:941	inverting dehydratases	920:941	Mg534 is a 4,6-dehydratase 5-epimerase; its three-dimensional structure suggests that it belongs to a third subfamily of inverting dehydratases.
25035429	7	43	theme	3-epimerase	990:1000	arg1	Mg535					944:948	Mg535	944:948	Mg535	944:948	Mg535, next in the pathway, is a bifunctional 3-epimerase 4-reductase.
25035429	7	43	theme	3-epimerase	990:1000	arg1	4-reductase					1002:1012	a bifunctional 3-epimerase 4-reductase	975:1012	a bifunctional 3-epimerase 4-reductase	975:1012	Mg535, next in the pathway, is a bifunctional 3-epimerase 4-reductase.
25035429	5	44	theme	activity	677:684	arg1	assays					686:691	activity assays	677:691	activity assays on two enzymes of the pathway with mass spectrometry	677:744	We combined activity assays on two enzymes of the pathway with mass spectrometry and NMR studies to characterize their specificities.
25035429	6	45	theme	4,6-dehydratase	810:824	arg1	5-epimerase					826:836	a 4,6-dehydratase 5-epimerase	808:836	a 4,6-dehydratase 5-epimerase	808:836	Mg534 is a 4,6-dehydratase 5-epimerase; its three-dimensional structure suggests that it belongs to a third subfamily of inverting dehydratases.
25035429	6	45	theme	4,6-dehydratase	810:824	arg1	Mg534					799:803	Mg534	799:803	Mg534	799:803	Mg534 is a 4,6-dehydratase 5-epimerase; its three-dimensional structure suggests that it belongs to a third subfamily of inverting dehydratases.
25035429	9	46	theme	giant	1131:1135	arg1	viruses					1137:1143	giant viruses	1131:1143	giant viruses performing their glycan synthesis using enzymes different from their cellular counterparts	1131:1234	This study is another example of giant viruses performing their glycan synthesis using enzymes different from their cellular counterparts, raising again the question of the origin of these pathways.
25035429	7	47	from	Mg535	944:948	arg1	pathway					963:969	the pathway	959:969	the pathway	959:969	Mg535, next in the pathway, is a bifunctional 3-epimerase 4-reductase.
25035429	0	48	theme	uncommon	69:76	arg1	sugars					88:93	uncommon acetamido sugars	69:93	uncommon acetamido sugars	69:93	Giant virus Megavirus chilensis encodes the biosynthetic pathway for uncommon acetamido sugars.
25035429	9	49	theme	viruses	1137:1143	arg1	example					1120:1126	another example	1112:1126	another example of giant viruses performing their glycan synthesis using enzymes different from their cellular counterparts	1112:1234	This study is another example of giant viruses performing their glycan synthesis using enzymes different from their cellular counterparts, raising again the question of the origin of these pathways.
25035429	1	50	theme	Acanthamoeba	233:244	arg1	sp.					246:248	Acanthamoeba sp.	233:248	Acanthamoeba sp.	233:248	Giant viruses mimicking microbes, by the sizes of their particles and the heavily glycosylated fibrils surrounding their capsids, infect Acanthamoeba sp., which are ubiquitous unicellular eukaryotes.
25035429	1	50	theme	Acanthamoeba	233:244	arg1	eukaryotes					284:293	ubiquitous unicellular eukaryotes	261:293	ubiquitous unicellular eukaryotes	261:293	Giant viruses mimicking microbes, by the sizes of their particles and the heavily glycosylated fibrils surrounding their capsids, infect Acanthamoeba sp., which are ubiquitous unicellular eukaryotes.
25035429	9	51	theme	origin	1271:1276	arg1	question					1255:1262	again the question	1245:1262	again the question of the origin of these pathways	1245:1294	This study is another example of giant viruses performing their glycan synthesis using enzymes different from their cellular counterparts, raising again the question of the origin of these pathways.
29138791	2	0	with	arabinose	320:328	arg1	ratio					386:390	a molar ratio	378:390	a molar ratio of 1.0 : 1.8 : 2.4 : 3.8 : 4.9 : 8.5	378:427	The monosaccharide composition of PMH was determined as glucose, xylose, arabinose, rhamnose, galactose and galacturonic acid with a molar ratio of 1.0 : 1.8 : 2.4 : 3.8 : 4.9 : 8.5.
29138791	7	1	theme	structural	1058:1067	arg1	characteristics					1094:1108	its structural, thermal and antioxidant characteristics	1054:1108	its structural, thermal and antioxidant characteristics	1054:1108	The results suggested that PMH could be used in functional foods due to its structural, thermal and antioxidant characteristics.
29138791	7	2	theme	due	1047:1049	arg1	foods					1041:1045	functional foods	1030:1045	functional foods due to its structural, thermal and antioxidant characteristics	1030:1108	The results suggested that PMH could be used in functional foods due to its structural, thermal and antioxidant characteristics.
29138791	4	3	theme	mol-1	718:722	arg1	Ea					702:703	Ea	702:703	Ea	702:703	The thermal analysis using the Flynn-Wall-Ozawa (FWO) method revealed that PMH had an apparent activation energy (Ea) of 173.1 kJ mol-1.
29138791	4	3	theme	mol-1	718:722	arg1	energy					694:699	an apparent activation energy	671:699	an apparent activation energy (Ea) of 173.1 kJ mol-1	671:722	The thermal analysis using the Flynn-Wall-Ozawa (FWO) method revealed that PMH had an apparent activation energy (Ea) of 173.1 kJ mol-1.
29138791	1	4	theme	seed	212:215	arg1	husks					217:221	the seed husks	208:221	the seed husks of Plantago asiatica L	208:244	A new heteropolysaccharide (PMH) with a molecular weight of 1.4 × 103 kDa was isolated from the seed husks of Plantago asiatica L.
29138791	0	5	theme	thermal	80:86	arg1	properties					104:113	its thermal and antioxidant properties	76:113	its thermal and antioxidant properties	76:113	A new heteropolysaccharide from the seed husks of Plantago asiatica L. with its thermal and antioxidant properties.
29138791	2	6	with	rhamnose	331:338	arg1	ratio					386:390	a molar ratio	378:390	a molar ratio of 1.0 : 1.8 : 2.4 : 3.8 : 4.9 : 8.5	378:427	The monosaccharide composition of PMH was determined as glucose, xylose, arabinose, rhamnose, galactose and galacturonic acid with a molar ratio of 1.0 : 1.8 : 2.4 : 3.8 : 4.9 : 8.5.
29138791	1	7	with	heteropolysaccharide	122:141	arg1	weight					166:171	a molecular weight	154:171	a molecular weight of 1.4 × 103 kDa	154:188	A new heteropolysaccharide (PMH) with a molecular weight of 1.4 × 103 kDa was isolated from the seed husks of Plantago asiatica L.
29138791	5	8	theme	°C	815:816	arg1	temperature					795:805	a temperature	793:805	a temperature of 91.1 °C with a dry weight loss of 31.1%	793:848	PMH experienced a major decomposition during the heating process at a temperature of 91.1 °C with a dry weight loss of 31.1%.
29138791	7	9	theme	functional	1030:1039	arg1	foods					1041:1045	functional foods	1030:1045	functional foods due to its structural, thermal and antioxidant characteristics	1030:1108	The results suggested that PMH could be used in functional foods due to its structural, thermal and antioxidant characteristics.
29138791	5	10	with	°C	815:816	arg1	loss					836:839	a dry weight loss	823:839	a dry weight loss of 31.1%	823:848	PMH experienced a major decomposition during the heating process at a temperature of 91.1 °C with a dry weight loss of 31.1%.
29138791	2	11	theme	PMH	281:283	arg1	rhamnose					331:338	rhamnose	331:338	rhamnose	331:338	The monosaccharide composition of PMH was determined as glucose, xylose, arabinose, rhamnose, galactose and galacturonic acid with a molar ratio of 1.0 : 1.8 : 2.4 : 3.8 : 4.9 : 8.5.
29138791	2	11	theme	PMH	281:283	arg1	composition					266:276	The monosaccharide composition	247:276	The monosaccharide composition of PMH	247:283	The monosaccharide composition of PMH was determined as glucose, xylose, arabinose, rhamnose, galactose and galacturonic acid with a molar ratio of 1.0 : 1.8 : 2.4 : 3.8 : 4.9 : 8.5.
29138791	2	11	theme	PMH	281:283	arg1	glucose					303:309	glucose	303:309	glucose	303:309	The monosaccharide composition of PMH was determined as glucose, xylose, arabinose, rhamnose, galactose and galacturonic acid with a molar ratio of 1.0 : 1.8 : 2.4 : 3.8 : 4.9 : 8.5.
29138791	2	11	theme	PMH	281:283	arg1	xylose					312:317	xylose	312:317	xylose	312:317	The monosaccharide composition of PMH was determined as glucose, xylose, arabinose, rhamnose, galactose and galacturonic acid with a molar ratio of 1.0 : 1.8 : 2.4 : 3.8 : 4.9 : 8.5.
29138791	2	11	theme	PMH	281:283	arg1	galactose					341:349	galactose	341:349	galactose	341:349	The monosaccharide composition of PMH was determined as glucose, xylose, arabinose, rhamnose, galactose and galacturonic acid with a molar ratio of 1.0 : 1.8 : 2.4 : 3.8 : 4.9 : 8.5.
29138791	2	11	theme	PMH	281:283	arg1	acid					368:371	galacturonic acid	355:371	galacturonic acid	355:371	The monosaccharide composition of PMH was determined as glucose, xylose, arabinose, rhamnose, galactose and galacturonic acid with a molar ratio of 1.0 : 1.8 : 2.4 : 3.8 : 4.9 : 8.5.
29138791	2	11	theme	PMH	281:283	arg1	arabinose					320:328	arabinose	320:328	arabinose	320:328	The monosaccharide composition of PMH was determined as glucose, xylose, arabinose, rhamnose, galactose and galacturonic acid with a molar ratio of 1.0 : 1.8 : 2.4 : 3.8 : 4.9 : 8.5.
29138791	0	12	theme	antioxidant	92:102	arg1	properties					104:113	its thermal and antioxidant properties	76:113	its thermal and antioxidant properties	76:113	A new heteropolysaccharide from the seed husks of Plantago asiatica L. with its thermal and antioxidant properties.
29138791	0	13	from	husks	41:45	arg1	heteropolysaccharide					6:25	A new heteropolysaccharide	0:25	A new heteropolysaccharide from the seed husks of Plantago asiatica L. with its thermal and antioxidant properties.	0:114	A new heteropolysaccharide from the seed husks of Plantago asiatica L. with its thermal and antioxidant properties.
29138791	7	14	used	used	1022:1025	arg2	PMH					1009:1011	PMH	1009:1011	PMH	1009:1011	The results suggested that PMH could be used in functional foods due to its structural, thermal and antioxidant characteristics.
29138791	2	15	theme	galacturonic	355:366	arg1	composition					266:276	The monosaccharide composition	247:276	The monosaccharide composition of PMH	247:283	The monosaccharide composition of PMH was determined as glucose, xylose, arabinose, rhamnose, galactose and galacturonic acid with a molar ratio of 1.0 : 1.8 : 2.4 : 3.8 : 4.9 : 8.5.
29138791	2	15	theme	galacturonic	355:366	arg1	acid					368:371	galacturonic acid	355:371	galacturonic acid	355:371	The monosaccharide composition of PMH was determined as glucose, xylose, arabinose, rhamnose, galactose and galacturonic acid with a molar ratio of 1.0 : 1.8 : 2.4 : 3.8 : 4.9 : 8.5.
29138791	5	16	theme	dry	825:827	arg1	loss					836:839	a dry weight loss	823:839	a dry weight loss of 31.1%	823:848	PMH experienced a major decomposition during the heating process at a temperature of 91.1 °C with a dry weight loss of 31.1%.
29138791	6	17	theme	commercial	909:918	arg1	psyllium					920:927	commercial psyllium	909:927	commercial psyllium	909:927	Moreover, PMH exhibited stronger antioxidant ability than commercial psyllium, partially due to its higher content of uronic acid.
29138791	6	18	theme	antioxidant	884:894	arg1	ability					896:902	stronger antioxidant ability	875:902	stronger antioxidant ability	875:902	Moreover, PMH exhibited stronger antioxidant ability than commercial psyllium, partially due to its higher content of uronic acid.
29138791	2	19	with	acid	368:371	arg1	ratio					386:390	a molar ratio	378:390	a molar ratio of 1.0 : 1.8 : 2.4 : 3.8 : 4.9 : 8.5	378:427	The monosaccharide composition of PMH was determined as glucose, xylose, arabinose, rhamnose, galactose and galacturonic acid with a molar ratio of 1.0 : 1.8 : 2.4 : 3.8 : 4.9 : 8.5.
29138791	5	20	theme	weight	829:834	arg1	loss					836:839	a dry weight loss	823:839	a dry weight loss of 31.1%	823:848	PMH experienced a major decomposition during the heating process at a temperature of 91.1 °C with a dry weight loss of 31.1%.
29138791	0	21	theme	new	2:4	arg1	heteropolysaccharide					6:25	A new heteropolysaccharide	0:25	A new heteropolysaccharide from the seed husks of Plantago asiatica L. with its thermal and antioxidant properties.	0:114	A new heteropolysaccharide from the seed husks of Plantago asiatica L. with its thermal and antioxidant properties.
29138791	2	22	dep	ratio	386:390	arg1	 1.8 					400:404	 1.8 	400:404	 1.8 	400:404	The monosaccharide composition of PMH was determined as glucose, xylose, arabinose, rhamnose, galactose and galacturonic acid with a molar ratio of 1.0 : 1.8 : 2.4 : 3.8 : 4.9 : 8.5.
29138791	2	22	dep	ratio	386:390	arg1	 4.9 					418:422	 4.9 	418:422	 4.9 	418:422	The monosaccharide composition of PMH was determined as glucose, xylose, arabinose, rhamnose, galactose and galacturonic acid with a molar ratio of 1.0 : 1.8 : 2.4 : 3.8 : 4.9 : 8.5.
29138791	2	22	dep	ratio	386:390	arg1	 3.8 					412:416	 3.8 	412:416	 3.8 	412:416	The monosaccharide composition of PMH was determined as glucose, xylose, arabinose, rhamnose, galactose and galacturonic acid with a molar ratio of 1.0 : 1.8 : 2.4 : 3.8 : 4.9 : 8.5.
29138791	2	22	dep	ratio	386:390	arg1	 2.4 					406:410	 2.4 	406:410	 2.4 	406:410	The monosaccharide composition of PMH was determined as glucose, xylose, arabinose, rhamnose, galactose and galacturonic acid with a molar ratio of 1.0 : 1.8 : 2.4 : 3.8 : 4.9 : 8.5.
29138791	2	22	dep	ratio	386:390	arg1	 8.5					424:427	 8.5	424:427	 8.5	424:427	The monosaccharide composition of PMH was determined as glucose, xylose, arabinose, rhamnose, galactose and galacturonic acid with a molar ratio of 1.0 : 1.8 : 2.4 : 3.8 : 4.9 : 8.5.
29138791	2	23	with	galactose	341:349	arg1	ratio					386:390	a molar ratio	378:390	a molar ratio of 1.0 : 1.8 : 2.4 : 3.8 : 4.9 : 8.5	378:427	The monosaccharide composition of PMH was determined as glucose, xylose, arabinose, rhamnose, galactose and galacturonic acid with a molar ratio of 1.0 : 1.8 : 2.4 : 3.8 : 4.9 : 8.5.
29138791	1	24	theme	molecular	156:164	arg1	weight					166:171	a molecular weight	154:171	a molecular weight of 1.4 × 103 kDa	154:188	A new heteropolysaccharide (PMH) with a molecular weight of 1.4 × 103 kDa was isolated from the seed husks of Plantago asiatica L.
29138791	2	25	theme	molar	380:384	arg1	ratio					386:390	a molar ratio	378:390	a molar ratio of 1.0 : 1.8 : 2.4 : 3.8 : 4.9 : 8.5	378:427	The monosaccharide composition of PMH was determined as glucose, xylose, arabinose, rhamnose, galactose and galacturonic acid with a molar ratio of 1.0 : 1.8 : 2.4 : 3.8 : 4.9 : 8.5.
29138791	1	26	theme	Plantago	226:233	arg1	L					244:244	Plantago asiatica L	226:244	Plantago asiatica L	226:244	A new heteropolysaccharide (PMH) with a molecular weight of 1.4 × 103 kDa was isolated from the seed husks of Plantago asiatica L.
29138791	4	27	theme	kJ	715:716	arg1	mol-1					718:722	173.1 kJ mol-1	709:722	173.1 kJ mol-1	709:722	The thermal analysis using the Flynn-Wall-Ozawa (FWO) method revealed that PMH had an apparent activation energy (Ea) of 173.1 kJ mol-1.
29138791	4	28	theme	Flynn-Wall-Ozawa	619:634	arg1	method					642:647	the Flynn-Wall-Ozawa (FWO) method	615:647	the Flynn-Wall-Ozawa (FWO) method	615:647	The thermal analysis using the Flynn-Wall-Ozawa (FWO) method revealed that PMH had an apparent activation energy (Ea) of 173.1 kJ mol-1.
29138791	1	29	theme	asiatica	235:242	arg1	L					244:244	Plantago asiatica L	226:244	Plantago asiatica L	226:244	A new heteropolysaccharide (PMH) with a molecular weight of 1.4 × 103 kDa was isolated from the seed husks of Plantago asiatica L.
29138791	2	30	theme	monosaccharide	251:264	arg1	rhamnose					331:338	rhamnose	331:338	rhamnose	331:338	The monosaccharide composition of PMH was determined as glucose, xylose, arabinose, rhamnose, galactose and galacturonic acid with a molar ratio of 1.0 : 1.8 : 2.4 : 3.8 : 4.9 : 8.5.
29138791	2	30	theme	monosaccharide	251:264	arg1	composition					266:276	The monosaccharide composition	247:276	The monosaccharide composition of PMH	247:283	The monosaccharide composition of PMH was determined as glucose, xylose, arabinose, rhamnose, galactose and galacturonic acid with a molar ratio of 1.0 : 1.8 : 2.4 : 3.8 : 4.9 : 8.5.
29138791	2	30	theme	monosaccharide	251:264	arg1	glucose					303:309	glucose	303:309	glucose	303:309	The monosaccharide composition of PMH was determined as glucose, xylose, arabinose, rhamnose, galactose and galacturonic acid with a molar ratio of 1.0 : 1.8 : 2.4 : 3.8 : 4.9 : 8.5.
29138791	2	30	theme	monosaccharide	251:264	arg1	xylose					312:317	xylose	312:317	xylose	312:317	The monosaccharide composition of PMH was determined as glucose, xylose, arabinose, rhamnose, galactose and galacturonic acid with a molar ratio of 1.0 : 1.8 : 2.4 : 3.8 : 4.9 : 8.5.
29138791	2	30	theme	monosaccharide	251:264	arg1	galactose					341:349	galactose	341:349	galactose	341:349	The monosaccharide composition of PMH was determined as glucose, xylose, arabinose, rhamnose, galactose and galacturonic acid with a molar ratio of 1.0 : 1.8 : 2.4 : 3.8 : 4.9 : 8.5.
29138791	2	30	theme	monosaccharide	251:264	arg1	acid					368:371	galacturonic acid	355:371	galacturonic acid	355:371	The monosaccharide composition of PMH was determined as glucose, xylose, arabinose, rhamnose, galactose and galacturonic acid with a molar ratio of 1.0 : 1.8 : 2.4 : 3.8 : 4.9 : 8.5.
29138791	2	30	theme	monosaccharide	251:264	arg1	arabinose					320:328	arabinose	320:328	arabinose	320:328	The monosaccharide composition of PMH was determined as glucose, xylose, arabinose, rhamnose, galactose and galacturonic acid with a molar ratio of 1.0 : 1.8 : 2.4 : 3.8 : 4.9 : 8.5.
29138791	3	31	attach	attached	558:565	arg2	1,3-α-d-Galp					517:528	1,3-α-d-Galp	517:528	1,3-α-d-Galp	517:528	The backbone of PMH consisted of 1,4-β-d-GalpA with the side chains mainly composed of 1,3-α-d-Galp and 1,2-α-d-Galp which were attached to the O-3 of GlapA.
29138791	3	31	attach	attached	558:565	arg2	1,2-α-d-Galp					534:545	1,2-α-d-Galp	534:545	1,2-α-d-Galp	534:545	The backbone of PMH consisted of 1,4-β-d-GalpA with the side chains mainly composed of 1,3-α-d-Galp and 1,2-α-d-Galp which were attached to the O-3 of GlapA.
29138791	3	31	attach	attached	558:565	arg1	O-3					574:576	the O-3	570:576	the O-3 of GlapA	570:585	The backbone of PMH consisted of 1,4-β-d-GalpA with the side chains mainly composed of 1,3-α-d-Galp and 1,2-α-d-Galp which were attached to the O-3 of GlapA.
29138791	1	32	theme	L	244:244	arg1	husks					217:221	the seed husks	208:221	the seed husks of Plantago asiatica L	208:244	A new heteropolysaccharide (PMH) with a molecular weight of 1.4 × 103 kDa was isolated from the seed husks of Plantago asiatica L.
29138791	0	33	dep	Plantago	50:57	arg1	L.					68:69	Plantago asiatica L.	50:69	Plantago asiatica L.	50:69	A new heteropolysaccharide from the seed husks of Plantago asiatica L. with its thermal and antioxidant properties.
29138791	1	34	theme	1.4	176:178	arg1	×					180:180	×	180:180	×	180:180	A new heteropolysaccharide (PMH) with a molecular weight of 1.4 × 103 kDa was isolated from the seed husks of Plantago asiatica L.
29138791	0	35	theme	seed	36:39	arg1	husks					41:45	the seed husks	32:45	the seed husks of Plantago asiatica L.	32:69	A new heteropolysaccharide from the seed husks of Plantago asiatica L. with its thermal and antioxidant properties.
29138791	7	36	theme	thermal	1070:1076	arg1	characteristics					1094:1108	its structural, thermal and antioxidant characteristics	1054:1108	its structural, thermal and antioxidant characteristics	1054:1108	The results suggested that PMH could be used in functional foods due to its structural, thermal and antioxidant characteristics.
29138791	3	37	theme	side	486:489	arg1	chains					491:496	the side chains	482:496	the side chains mainly composed of 1,3-α-d-Galp and 1,2-α-d-Galp which were attached to the O-3 of GlapA	482:585	The backbone of PMH consisted of 1,4-β-d-GalpA with the side chains mainly composed of 1,3-α-d-Galp and 1,2-α-d-Galp which were attached to the O-3 of GlapA.
29138791	6	38	theme	stronger	875:882	arg1	ability					896:902	stronger antioxidant ability	875:902	stronger antioxidant ability	875:902	Moreover, PMH exhibited stronger antioxidant ability than commercial psyllium, partially due to its higher content of uronic acid.
29138791	1	39	theme	103	182:184	arg1	×					180:180	×	180:180	×	180:180	A new heteropolysaccharide (PMH) with a molecular weight of 1.4 × 103 kDa was isolated from the seed husks of Plantago asiatica L.
29138791	2	40	theme	1.0 	395:398	arg1	ratio					386:390	a molar ratio	378:390	a molar ratio of 1.0 : 1.8 : 2.4 : 3.8 : 4.9 : 8.5	378:427	The monosaccharide composition of PMH was determined as glucose, xylose, arabinose, rhamnose, galactose and galacturonic acid with a molar ratio of 1.0 : 1.8 : 2.4 : 3.8 : 4.9 : 8.5.
29138791	6	41	theme	uronic	969:974	arg1	acid					976:979	uronic acid	969:979	uronic acid	969:979	Moreover, PMH exhibited stronger antioxidant ability than commercial psyllium, partially due to its higher content of uronic acid.
29138791	0	42	with	heteropolysaccharide	6:25	arg1	properties					104:113	its thermal and antioxidant properties	76:113	its thermal and antioxidant properties	76:113	A new heteropolysaccharide from the seed husks of Plantago asiatica L. with its thermal and antioxidant properties.
29138791	5	43	theme	heating	774:780	arg1	process					782:788	the heating process	770:788	the heating process at a temperature of 91.1 °C with a dry weight loss of 31.1%	770:848	PMH experienced a major decomposition during the heating process at a temperature of 91.1 °C with a dry weight loss of 31.1%.
29138791	3	44	theme	GlapA	581:585	arg1	O-3					574:576	the O-3	570:576	the O-3 of GlapA	570:585	The backbone of PMH consisted of 1,4-β-d-GalpA with the side chains mainly composed of 1,3-α-d-Galp and 1,2-α-d-Galp which were attached to the O-3 of GlapA.
29138791	1	45	attach	isolated	194:201	arg2	PMH					144:146	PMH	144:146	PMH	144:146	A new heteropolysaccharide (PMH) with a molecular weight of 1.4 × 103 kDa was isolated from the seed husks of Plantago asiatica L.
29138791	1	45	attach	isolated	194:201	arg1	husks					217:221	the seed husks	208:221	the seed husks of Plantago asiatica L	208:244	A new heteropolysaccharide (PMH) with a molecular weight of 1.4 × 103 kDa was isolated from the seed husks of Plantago asiatica L.
29138791	1	45	attach	isolated	194:201	arg2	heteropolysaccharide					122:141	A new heteropolysaccharide	116:141	A new heteropolysaccharide (PMH) with a molecular weight of 1.4 × 103 kDa	116:188	A new heteropolysaccharide (PMH) with a molecular weight of 1.4 × 103 kDa was isolated from the seed husks of Plantago asiatica L.
29138791	4	46	theme	thermal	592:598	arg1	analysis					600:607	The thermal analysis	588:607	The thermal analysis using the Flynn-Wall-Ozawa (FWO) method	588:647	The thermal analysis using the Flynn-Wall-Ozawa (FWO) method revealed that PMH had an apparent activation energy (Ea) of 173.1 kJ mol-1.
29138791	5	47	theme	%	848:848	arg1	loss					836:839	a dry weight loss	823:839	a dry weight loss of 31.1%	823:848	PMH experienced a major decomposition during the heating process at a temperature of 91.1 °C with a dry weight loss of 31.1%.
29138791	2	48	with	glucose	303:309	arg1	ratio					386:390	a molar ratio	378:390	a molar ratio of 1.0 : 1.8 : 2.4 : 3.8 : 4.9 : 8.5	378:427	The monosaccharide composition of PMH was determined as glucose, xylose, arabinose, rhamnose, galactose and galacturonic acid with a molar ratio of 1.0 : 1.8 : 2.4 : 3.8 : 4.9 : 8.5.
29138791	7	49	theme	antioxidant	1082:1092	arg1	characteristics					1094:1108	its structural, thermal and antioxidant characteristics	1054:1108	its structural, thermal and antioxidant characteristics	1054:1108	The results suggested that PMH could be used in functional foods due to its structural, thermal and antioxidant characteristics.
29138791	3	50	theme	PMH	446:448	arg1	backbone					434:441	The backbone	430:441	The backbone of PMH	430:448	The backbone of PMH consisted of 1,4-β-d-GalpA with the side chains mainly composed of 1,3-α-d-Galp and 1,2-α-d-Galp which were attached to the O-3 of GlapA.
29138791	1	51	theme	kDa	186:188	arg1	weight					166:171	a molecular weight	154:171	a molecular weight of 1.4 × 103 kDa	154:188	A new heteropolysaccharide (PMH) with a molecular weight of 1.4 × 103 kDa was isolated from the seed husks of Plantago asiatica L.
29138791	6	52	theme	higher	951:956	arg1	content					958:964	its higher content	947:964	its higher content of uronic acid	947:979	Moreover, PMH exhibited stronger antioxidant ability than commercial psyllium, partially due to its higher content of uronic acid.
29138791	1	53	theme	new	118:120	arg1	PMH					144:146	PMH	144:146	PMH	144:146	A new heteropolysaccharide (PMH) with a molecular weight of 1.4 × 103 kDa was isolated from the seed husks of Plantago asiatica L.
29138791	1	53	theme	new	118:120	arg1	heteropolysaccharide					122:141	A new heteropolysaccharide	116:141	A new heteropolysaccharide (PMH) with a molecular weight of 1.4 × 103 kDa	116:188	A new heteropolysaccharide (PMH) with a molecular weight of 1.4 × 103 kDa was isolated from the seed husks of Plantago asiatica L.
29138791	5	54	from	temperature	795:805	arg1	process					782:788	the heating process	770:788	the heating process at a temperature of 91.1 °C with a dry weight loss of 31.1%	770:848	PMH experienced a major decomposition during the heating process at a temperature of 91.1 °C with a dry weight loss of 31.1%.
29138791	2	55	with	xylose	312:317	arg1	ratio					386:390	a molar ratio	378:390	a molar ratio of 1.0 : 1.8 : 2.4 : 3.8 : 4.9 : 8.5	378:427	The monosaccharide composition of PMH was determined as glucose, xylose, arabinose, rhamnose, galactose and galacturonic acid with a molar ratio of 1.0 : 1.8 : 2.4 : 3.8 : 4.9 : 8.5.
29138791	4	56	theme	activation	683:692	arg1	Ea					702:703	Ea	702:703	Ea	702:703	The thermal analysis using the Flynn-Wall-Ozawa (FWO) method revealed that PMH had an apparent activation energy (Ea) of 173.1 kJ mol-1.
29138791	4	56	theme	activation	683:692	arg1	energy					694:699	an apparent activation energy	671:699	an apparent activation energy (Ea) of 173.1 kJ mol-1	671:722	The thermal analysis using the Flynn-Wall-Ozawa (FWO) method revealed that PMH had an apparent activation energy (Ea) of 173.1 kJ mol-1.
29138791	5	57	theme	major	743:747	arg1	decomposition					749:761	a major decomposition	741:761	a major decomposition	741:761	PMH experienced a major decomposition during the heating process at a temperature of 91.1 °C with a dry weight loss of 31.1%.
29138791	4	58	contain	had	667:669	arg2	Ea					702:703	Ea	702:703	Ea	702:703	The thermal analysis using the Flynn-Wall-Ozawa (FWO) method revealed that PMH had an apparent activation energy (Ea) of 173.1 kJ mol-1.
29138791	4	58	contain	had	667:669	arg2	energy					694:699	an apparent activation energy	671:699	an apparent activation energy (Ea) of 173.1 kJ mol-1	671:722	The thermal analysis using the Flynn-Wall-Ozawa (FWO) method revealed that PMH had an apparent activation energy (Ea) of 173.1 kJ mol-1.
29138791	4	58	contain	had	667:669	arg1	PMH					663:665	PMH	663:665	PMH	663:665	The thermal analysis using the Flynn-Wall-Ozawa (FWO) method revealed that PMH had an apparent activation energy (Ea) of 173.1 kJ mol-1.
29138791	0	59	theme	Plantago	50:57	arg1	husks					41:45	the seed husks	32:45	the seed husks of Plantago asiatica L.	32:69	A new heteropolysaccharide from the seed husks of Plantago asiatica L. with its thermal and antioxidant properties.
29138791	6	60	theme	acid	976:979	arg1	content					958:964	its higher content	947:964	its higher content of uronic acid	947:979	Moreover, PMH exhibited stronger antioxidant ability than commercial psyllium, partially due to its higher content of uronic acid.
29138791	4	61	theme	apparent	674:681	arg1	Ea					702:703	Ea	702:703	Ea	702:703	The thermal analysis using the Flynn-Wall-Ozawa (FWO) method revealed that PMH had an apparent activation energy (Ea) of 173.1 kJ mol-1.
29138791	4	61	theme	apparent	674:681	arg1	energy					694:699	an apparent activation energy	671:699	an apparent activation energy (Ea) of 173.1 kJ mol-1	671:722	The thermal analysis using the Flynn-Wall-Ozawa (FWO) method revealed that PMH had an apparent activation energy (Ea) of 173.1 kJ mol-1.
28917894	7	0	theme	carbohydrate	1140:1151	arg1	nature					1153:1158	the carbohydrate nature	1136:1158	the carbohydrate nature of polysaccharide	1136:1176	Fourier transformed infrared spectroscopy (FTIR) study confirms the carbohydrate nature of polysaccharide.
28917894	2	1	theme	molecular	562:570	arg1	1.98×102kDa					613:623	1.98×102kDa	613:623	1.98×102kDa	613:623	The apparent molecular weight of polysaccharide was found to be 1.98×102kDa.
28917894	2	1	theme	molecular	562:570	arg1	weight					572:577	The apparent molecular weight	549:577	The apparent molecular weight of polysaccharide	549:595	The apparent molecular weight of polysaccharide was found to be 1.98×102kDa.
28917894	1	2	theme	release	331:337	arg1	study					339:343	structural, functional and drug release study	299:343	structural, functional and drug release study of water soluble polysaccharide (ALPS) from seeds of Albizia lebbeck L. High swelling, water holding capacity, foam stability and lower moisture content suggests its use as additive in food preparations	299:546	Therefore, the current paper presents the studies on structural, functional and drug release study of water soluble polysaccharide (ALPS) from seeds of Albizia lebbeck L. High swelling, water holding capacity, foam stability and lower moisture content suggests its use as additive in food preparations.
28917894	0	3	theme	polysaccharide	81:94	arg1	characteristics					48:62	Structural, functional and pH sensitive release characteristics	0:62	Structural, functional and pH sensitive release characteristics of water-soluble polysaccharide from the seeds of Albizia lebbeck L. Plant polysaccharides, generally regarded as safe (GRAS),	0:189	Structural, functional and pH sensitive release characteristics of water-soluble polysaccharide from the seeds of Albizia lebbeck L. Plant polysaccharides, generally regarded as safe (GRAS), are gaining importance as excipients in drug delivery.
28917894	2	4	theme	apparent	553:560	arg1	1.98×102kDa					613:623	1.98×102kDa	613:623	1.98×102kDa	613:623	The apparent molecular weight of polysaccharide was found to be 1.98×102kDa.
28917894	2	4	theme	apparent	553:560	arg1	weight					572:577	The apparent molecular weight	549:577	The apparent molecular weight of polysaccharide	549:595	The apparent molecular weight of polysaccharide was found to be 1.98×102kDa.
28917894	1	5	theme	drug	326:329	arg1	release					331:337	drug release	326:337	drug release	326:337	Therefore, the current paper presents the studies on structural, functional and drug release study of water soluble polysaccharide (ALPS) from seeds of Albizia lebbeck L. High swelling, water holding capacity, foam stability and lower moisture content suggests its use as additive in food preparations.
28917894	0	6	theme	drug	231:234	arg1	delivery					236:243	drug delivery	231:243	drug delivery	231:243	Structural, functional and pH sensitive release characteristics of water-soluble polysaccharide from the seeds of Albizia lebbeck L. Plant polysaccharides, generally regarded as safe (GRAS), are gaining importance as excipients in drug delivery.
28917894	9	7	theme	polysaccharide	1346:1359	arg1	use					1339:1341	the use	1335:1341	the use of polysaccharide for the preparation of pH sensitive pharmaceutical dosage forms	1335:1423	The drug release profile reveals the use of polysaccharide for the preparation of pH sensitive pharmaceutical dosage forms.
28917894	0	8	from	characteristics	48:62	arg1	seeds					105:109	the seeds	101:109	the seeds of Albizia lebbeck L. Plant polysaccharides, generally regarded as safe (GRAS),	101:189	Structural, functional and pH sensitive release characteristics of water-soluble polysaccharide from the seeds of Albizia lebbeck L. Plant polysaccharides, generally regarded as safe (GRAS), are gaining importance as excipients in drug delivery.
28917894	1	9	from	studies	288:294	arg1	study					339:343	structural, functional and drug release study	299:343	structural, functional and drug release study of water soluble polysaccharide (ALPS) from seeds of Albizia lebbeck L. High swelling, water holding capacity, foam stability and lower moisture content suggests its use as additive in food preparations	299:546	Therefore, the current paper presents the studies on structural, functional and drug release study of water soluble polysaccharide (ALPS) from seeds of Albizia lebbeck L. High swelling, water holding capacity, foam stability and lower moisture content suggests its use as additive in food preparations.
28917894	1	10	theme	L.	414:415	arg1	capacity					446:453	water holding capacity	432:453	water holding capacity	432:453	Therefore, the current paper presents the studies on structural, functional and drug release study of water soluble polysaccharide (ALPS) from seeds of Albizia lebbeck L. High swelling, water holding capacity, foam stability and lower moisture content suggests its use as additive in food preparations.
28917894	1	10	theme	L.	414:415	arg1	stability					461:469	foam stability	456:469	foam stability	456:469	Therefore, the current paper presents the studies on structural, functional and drug release study of water soluble polysaccharide (ALPS) from seeds of Albizia lebbeck L. High swelling, water holding capacity, foam stability and lower moisture content suggests its use as additive in food preparations.
28917894	1	10	theme	L.	414:415	arg1	swelling					422:429	Albizia lebbeck L. High swelling	398:429	Albizia lebbeck L. High swelling	398:429	Therefore, the current paper presents the studies on structural, functional and drug release study of water soluble polysaccharide (ALPS) from seeds of Albizia lebbeck L. High swelling, water holding capacity, foam stability and lower moisture content suggests its use as additive in food preparations.
28917894	1	10	theme	L.	414:415	arg1	content					490:496	lower moisture content	475:496	lower moisture content	475:496	Therefore, the current paper presents the studies on structural, functional and drug release study of water soluble polysaccharide (ALPS) from seeds of Albizia lebbeck L. High swelling, water holding capacity, foam stability and lower moisture content suggests its use as additive in food preparations.
28917894	0	11	theme	Albizia	114:120	arg1	polysaccharides					139:153	Albizia lebbeck L. Plant polysaccharides	114:153	Albizia lebbeck L. Plant polysaccharides	114:153	Structural, functional and pH sensitive release characteristics of water-soluble polysaccharide from the seeds of Albizia lebbeck L. Plant polysaccharides, generally regarded as safe (GRAS), are gaining importance as excipients in drug delivery.
28917894	1	12	dep	structural	299:308	arg1	functional					311:320	functional	311:320	functional	311:320	Therefore, the current paper presents the studies on structural, functional and drug release study of water soluble polysaccharide (ALPS) from seeds of Albizia lebbeck L. High swelling, water holding capacity, foam stability and lower moisture content suggests its use as additive in food preparations.
28917894	1	13	theme	High	417:420	arg1	capacity					446:453	water holding capacity	432:453	water holding capacity	432:453	Therefore, the current paper presents the studies on structural, functional and drug release study of water soluble polysaccharide (ALPS) from seeds of Albizia lebbeck L. High swelling, water holding capacity, foam stability and lower moisture content suggests its use as additive in food preparations.
28917894	1	13	theme	High	417:420	arg1	stability					461:469	foam stability	456:469	foam stability	456:469	Therefore, the current paper presents the studies on structural, functional and drug release study of water soluble polysaccharide (ALPS) from seeds of Albizia lebbeck L. High swelling, water holding capacity, foam stability and lower moisture content suggests its use as additive in food preparations.
28917894	1	13	theme	High	417:420	arg1	swelling					422:429	Albizia lebbeck L. High swelling	398:429	Albizia lebbeck L. High swelling	398:429	Therefore, the current paper presents the studies on structural, functional and drug release study of water soluble polysaccharide (ALPS) from seeds of Albizia lebbeck L. High swelling, water holding capacity, foam stability and lower moisture content suggests its use as additive in food preparations.
28917894	1	13	theme	High	417:420	arg1	content					490:496	lower moisture content	475:496	lower moisture content	475:496	Therefore, the current paper presents the studies on structural, functional and drug release study of water soluble polysaccharide (ALPS) from seeds of Albizia lebbeck L. High swelling, water holding capacity, foam stability and lower moisture content suggests its use as additive in food preparations.
28917894	8	14	theme	polysaccharide	1274:1287	arg1	degradation					1289:1299	polysaccharide degradation	1274:1299	polysaccharide degradation	1274:1299	From the thermogravimetric analysis (TGA) data, the second mass loss (243-340°C) attributed to polysaccharide degradation.
28917894	8	15	theme	thermogravimetric	1188:1204	arg1	TGA					1216:1218	TGA	1216:1218	TGA	1216:1218	From the thermogravimetric analysis (TGA) data, the second mass loss (243-340°C) attributed to polysaccharide degradation.
28917894	8	15	theme	thermogravimetric	1188:1204	arg1	analysis					1206:1213	thermogravimetric analysis	1188:1213	the thermogravimetric analysis (TGA) data	1184:1224	From the thermogravimetric analysis (TGA) data, the second mass loss (243-340°C) attributed to polysaccharide degradation.
28917894	9	16	theme	sensitive	1387:1395	arg1	forms					1419:1423	pH sensitive pharmaceutical dosage forms	1384:1423	pH sensitive pharmaceutical dosage forms	1384:1423	The drug release profile reveals the use of polysaccharide for the preparation of pH sensitive pharmaceutical dosage forms.
28917894	2	17	theme	polysaccharide	582:595	arg1	1.98×102kDa					613:623	1.98×102kDa	613:623	1.98×102kDa	613:623	The apparent molecular weight of polysaccharide was found to be 1.98×102kDa.
28917894	2	17	theme	polysaccharide	582:595	arg1	weight					572:577	The apparent molecular weight	549:577	The apparent molecular weight of polysaccharide	549:595	The apparent molecular weight of polysaccharide was found to be 1.98×102kDa.
28917894	1	18	theme	swelling	422:429	arg1	seeds					389:393	seeds	389:393	seeds of Albizia lebbeck L. High swelling, water holding capacity, foam stability and lower moisture content suggests its use as additive in food preparations	389:546	Therefore, the current paper presents the studies on structural, functional and drug release study of water soluble polysaccharide (ALPS) from seeds of Albizia lebbeck L. High swelling, water holding capacity, foam stability and lower moisture content suggests its use as additive in food preparations.
28917894	1	19	dep	capacity	446:453	arg1	suggests					498:505	suggests	498:505	suggests its use as additive in food preparations	498:546	Therefore, the current paper presents the studies on structural, functional and drug release study of water soluble polysaccharide (ALPS) from seeds of Albizia lebbeck L. High swelling, water holding capacity, foam stability and lower moisture content suggests its use as additive in food preparations.
28917894	8	20	theme	second	1231:1236	arg1	loss					1243:1246	the second mass loss	1227:1246	the second mass loss (243-340°C)	1227:1258	From the thermogravimetric analysis (TGA) data, the second mass loss (243-340°C) attributed to polysaccharide degradation.
28917894	8	20	theme	second	1231:1236	arg1	243-340°C					1249:1257	243-340°C	1249:1257	243-340°C	1249:1257	From the thermogravimetric analysis (TGA) data, the second mass loss (243-340°C) attributed to polysaccharide degradation.
28917894	1	21	from	seeds	389:393	arg1	study					339:343	structural, functional and drug release study	299:343	structural, functional and drug release study of water soluble polysaccharide (ALPS) from seeds of Albizia lebbeck L. High swelling, water holding capacity, foam stability and lower moisture content suggests its use as additive in food preparations	299:546	Therefore, the current paper presents the studies on structural, functional and drug release study of water soluble polysaccharide (ALPS) from seeds of Albizia lebbeck L. High swelling, water holding capacity, foam stability and lower moisture content suggests its use as additive in food preparations.
28917894	1	21	from	seeds	389:393	arg1	ALPS					378:381	ALPS	378:381	ALPS	378:381	Therefore, the current paper presents the studies on structural, functional and drug release study of water soluble polysaccharide (ALPS) from seeds of Albizia lebbeck L. High swelling, water holding capacity, foam stability and lower moisture content suggests its use as additive in food preparations.
28917894	1	21	from	seeds	389:393	arg1	polysaccharide					362:375	water soluble polysaccharide	348:375	water soluble polysaccharide (ALPS) from seeds of Albizia lebbeck L. High swelling, water holding capacity, foam stability and lower moisture content suggests its use as additive in food preparations	348:546	Therefore, the current paper presents the studies on structural, functional and drug release study of water soluble polysaccharide (ALPS) from seeds of Albizia lebbeck L. High swelling, water holding capacity, foam stability and lower moisture content suggests its use as additive in food preparations.
28917894	6	22	theme	Morphological	976:988	arg1	study					990:994	Morphological study	976:994	Morphological study	976:994	Morphological study reveals the polysaccharide with irregular particle shape and rough surface.
28917894	0	23	theme	Structural	0:9	arg1	characteristics					48:62	Structural, functional and pH sensitive release characteristics	0:62	Structural, functional and pH sensitive release characteristics of water-soluble polysaccharide from the seeds of Albizia lebbeck L. Plant polysaccharides, generally regarded as safe (GRAS),	0:189	Structural, functional and pH sensitive release characteristics of water-soluble polysaccharide from the seeds of Albizia lebbeck L. Plant polysaccharides, generally regarded as safe (GRAS), are gaining importance as excipients in drug delivery.
28917894	7	24	theme	Fourier	1072:1078	arg1	study					1121:1125	Fourier transformed infrared spectroscopy (FTIR) study	1072:1125	Fourier transformed infrared spectroscopy (FTIR) study	1072:1125	Fourier transformed infrared spectroscopy (FTIR) study confirms the carbohydrate nature of polysaccharide.
28917894	1	25	theme	water	432:436	arg1	capacity					446:453	water holding capacity	432:453	water holding capacity	432:453	Therefore, the current paper presents the studies on structural, functional and drug release study of water soluble polysaccharide (ALPS) from seeds of Albizia lebbeck L. High swelling, water holding capacity, foam stability and lower moisture content suggests its use as additive in food preparations.
28917894	1	25	theme	water	432:436	arg1	swelling					422:429	Albizia lebbeck L. High swelling	398:429	Albizia lebbeck L. High swelling	398:429	Therefore, the current paper presents the studies on structural, functional and drug release study of water soluble polysaccharide (ALPS) from seeds of Albizia lebbeck L. High swelling, water holding capacity, foam stability and lower moisture content suggests its use as additive in food preparations.
28917894	7	26	theme	infrared	1092:1099	arg1	spectroscopy					1101:1112	infrared spectroscopy	1092:1112	Fourier transformed infrared spectroscopy (FTIR) study	1072:1125	Fourier transformed infrared spectroscopy (FTIR) study confirms the carbohydrate nature of polysaccharide.
28917894	7	26	theme	infrared	1092:1099	arg1	FTIR					1115:1118	FTIR	1115:1118	FTIR	1115:1118	Fourier transformed infrared spectroscopy (FTIR) study confirms the carbohydrate nature of polysaccharide.
28917894	1	27	theme	current	261:267	arg1	paper					269:273	the current paper	257:273	the current paper	257:273	Therefore, the current paper presents the studies on structural, functional and drug release study of water soluble polysaccharide (ALPS) from seeds of Albizia lebbeck L. High swelling, water holding capacity, foam stability and lower moisture content suggests its use as additive in food preparations.
28917894	0	28	theme	L.	130:131	arg1	polysaccharides					139:153	Albizia lebbeck L. Plant polysaccharides	114:153	Albizia lebbeck L. Plant polysaccharides	114:153	Structural, functional and pH sensitive release characteristics of water-soluble polysaccharide from the seeds of Albizia lebbeck L. Plant polysaccharides, generally regarded as safe (GRAS), are gaining importance as excipients in drug delivery.
28917894	1	29	theme	holding	438:444	arg1	capacity					446:453	water holding capacity	432:453	water holding capacity	432:453	Therefore, the current paper presents the studies on structural, functional and drug release study of water soluble polysaccharide (ALPS) from seeds of Albizia lebbeck L. High swelling, water holding capacity, foam stability and lower moisture content suggests its use as additive in food preparations.
28917894	1	29	theme	holding	438:444	arg1	swelling					422:429	Albizia lebbeck L. High swelling	398:429	Albizia lebbeck L. High swelling	398:429	Therefore, the current paper presents the studies on structural, functional and drug release study of water soluble polysaccharide (ALPS) from seeds of Albizia lebbeck L. High swelling, water holding capacity, foam stability and lower moisture content suggests its use as additive in food preparations.
28917894	9	30	theme	drug	1306:1309	arg1	profile					1319:1325	The drug release profile	1302:1325	The drug release profile	1302:1325	The drug release profile reveals the use of polysaccharide for the preparation of pH sensitive pharmaceutical dosage forms.
28917894	9	31	theme	pharmaceutical	1397:1410	arg1	forms					1419:1423	pH sensitive pharmaceutical dosage forms	1384:1423	pH sensitive pharmaceutical dosage forms	1384:1423	The drug release profile reveals the use of polysaccharide for the preparation of pH sensitive pharmaceutical dosage forms.
28917894	0	32	theme	functional	12:21	arg1	characteristics					48:62	Structural, functional and pH sensitive release characteristics	0:62	Structural, functional and pH sensitive release characteristics of water-soluble polysaccharide from the seeds of Albizia lebbeck L. Plant polysaccharides, generally regarded as safe (GRAS),	0:189	Structural, functional and pH sensitive release characteristics of water-soluble polysaccharide from the seeds of Albizia lebbeck L. Plant polysaccharides, generally regarded as safe (GRAS), are gaining importance as excipients in drug delivery.
28917894	0	33	theme	lebbeck	122:128	arg1	polysaccharides					139:153	Albizia lebbeck L. Plant polysaccharides	114:153	Albizia lebbeck L. Plant polysaccharides	114:153	Structural, functional and pH sensitive release characteristics of water-soluble polysaccharide from the seeds of Albizia lebbeck L. Plant polysaccharides, generally regarded as safe (GRAS), are gaining importance as excipients in drug delivery.
28917894	1	34	theme	soluble	354:360	arg1	ALPS					378:381	ALPS	378:381	ALPS	378:381	Therefore, the current paper presents the studies on structural, functional and drug release study of water soluble polysaccharide (ALPS) from seeds of Albizia lebbeck L. High swelling, water holding capacity, foam stability and lower moisture content suggests its use as additive in food preparations.
28917894	1	34	theme	soluble	354:360	arg1	polysaccharide					362:375	water soluble polysaccharide	348:375	water soluble polysaccharide (ALPS) from seeds of Albizia lebbeck L. High swelling, water holding capacity, foam stability and lower moisture content suggests its use as additive in food preparations	348:546	Therefore, the current paper presents the studies on structural, functional and drug release study of water soluble polysaccharide (ALPS) from seeds of Albizia lebbeck L. High swelling, water holding capacity, foam stability and lower moisture content suggests its use as additive in food preparations.
28917894	1	35	theme	food	530:533	arg1	preparations					535:546	food preparations	530:546	food preparations	530:546	Therefore, the current paper presents the studies on structural, functional and drug release study of water soluble polysaccharide (ALPS) from seeds of Albizia lebbeck L. High swelling, water holding capacity, foam stability and lower moisture content suggests its use as additive in food preparations.
28917894	8	36	theme	analysis	1206:1213	arg1	data					1221:1224	the thermogravimetric analysis (TGA) data	1184:1224	the thermogravimetric analysis (TGA) data	1184:1224	From the thermogravimetric analysis (TGA) data, the second mass loss (243-340°C) attributed to polysaccharide degradation.
28917894	0	37	theme	sensitive	30:38	arg1	characteristics					48:62	Structural, functional and pH sensitive release characteristics	0:62	Structural, functional and pH sensitive release characteristics of water-soluble polysaccharide from the seeds of Albizia lebbeck L. Plant polysaccharides, generally regarded as safe (GRAS),	0:189	Structural, functional and pH sensitive release characteristics of water-soluble polysaccharide from the seeds of Albizia lebbeck L. Plant polysaccharides, generally regarded as safe (GRAS), are gaining importance as excipients in drug delivery.
28917894	0	38	theme	polysaccharides	139:153	arg1	seeds					105:109	the seeds	101:109	the seeds of Albizia lebbeck L. Plant polysaccharides, generally regarded as safe (GRAS),	101:189	Structural, functional and pH sensitive release characteristics of water-soluble polysaccharide from the seeds of Albizia lebbeck L. Plant polysaccharides, generally regarded as safe (GRAS), are gaining importance as excipients in drug delivery.
28917894	1	39	theme	polysaccharide	362:375	arg1	study					339:343	structural, functional and drug release study	299:343	structural, functional and drug release study of water soluble polysaccharide (ALPS) from seeds of Albizia lebbeck L. High swelling, water holding capacity, foam stability and lower moisture content suggests its use as additive in food preparations	299:546	Therefore, the current paper presents the studies on structural, functional and drug release study of water soluble polysaccharide (ALPS) from seeds of Albizia lebbeck L. High swelling, water holding capacity, foam stability and lower moisture content suggests its use as additive in food preparations.
28917894	9	40	theme	dosage	1412:1417	arg1	forms					1419:1423	pH sensitive pharmaceutical dosage forms	1384:1423	pH sensitive pharmaceutical dosage forms	1384:1423	The drug release profile reveals the use of polysaccharide for the preparation of pH sensitive pharmaceutical dosage forms.
28917894	7	41	theme	transformed	1080:1090	arg1	study					1121:1125	Fourier transformed infrared spectroscopy (FTIR) study	1072:1125	Fourier transformed infrared spectroscopy (FTIR) study	1072:1125	Fourier transformed infrared spectroscopy (FTIR) study confirms the carbohydrate nature of polysaccharide.
28917894	0	42	theme	Plant	133:137	arg1	polysaccharides					139:153	Albizia lebbeck L. Plant polysaccharides	114:153	Albizia lebbeck L. Plant polysaccharides	114:153	Structural, functional and pH sensitive release characteristics of water-soluble polysaccharide from the seeds of Albizia lebbeck L. Plant polysaccharides, generally regarded as safe (GRAS), are gaining importance as excipients in drug delivery.
28917894	8	43	theme	mass	1238:1241	arg1	loss					1243:1246	the second mass loss	1227:1246	the second mass loss (243-340°C)	1227:1258	From the thermogravimetric analysis (TGA) data, the second mass loss (243-340°C) attributed to polysaccharide degradation.
28917894	8	43	theme	mass	1238:1241	arg1	243-340°C					1249:1257	243-340°C	1249:1257	243-340°C	1249:1257	From the thermogravimetric analysis (TGA) data, the second mass loss (243-340°C) attributed to polysaccharide degradation.
28917894	0	44	dep	safe	178:181	arg1	GRAS					184:187	GRAS	184:187	GRAS	184:187	Structural, functional and pH sensitive release characteristics of water-soluble polysaccharide from the seeds of Albizia lebbeck L. Plant polysaccharides, generally regarded as safe (GRAS), are gaining importance as excipients in drug delivery.
28917894	7	45	theme	polysaccharide	1163:1176	arg1	nature					1153:1158	the carbohydrate nature	1136:1158	the carbohydrate nature of polysaccharide	1136:1176	Fourier transformed infrared spectroscopy (FTIR) study confirms the carbohydrate nature of polysaccharide.
28917894	6	46	theme	rough	1057:1061	arg1	surface					1063:1069	rough surface	1057:1069	rough surface	1057:1069	Morphological study reveals the polysaccharide with irregular particle shape and rough surface.
28917894	0	47	theme	release	40:46	arg1	characteristics					48:62	Structural, functional and pH sensitive release characteristics	0:62	Structural, functional and pH sensitive release characteristics of water-soluble polysaccharide from the seeds of Albizia lebbeck L. Plant polysaccharides, generally regarded as safe (GRAS),	0:189	Structural, functional and pH sensitive release characteristics of water-soluble polysaccharide from the seeds of Albizia lebbeck L. Plant polysaccharides, generally regarded as safe (GRAS), are gaining importance as excipients in drug delivery.
28917894	3	48	theme	composition	641:651	arg1	analysis					653:660	Monosaccharide composition analysis	626:660	Monosaccharide composition analysis	626:660	Monosaccharide composition analysis indicated that ALPS consists of mannose (4.06%), rhamnose (22.79%), glucose (38.9%), galactose (17.84%) and xylose (16.42%).
28917894	1	49	theme	foam	456:459	arg1	stability					461:469	foam stability	456:469	foam stability	456:469	Therefore, the current paper presents the studies on structural, functional and drug release study of water soluble polysaccharide (ALPS) from seeds of Albizia lebbeck L. High swelling, water holding capacity, foam stability and lower moisture content suggests its use as additive in food preparations.
28917894	1	49	theme	foam	456:459	arg1	swelling					422:429	Albizia lebbeck L. High swelling	398:429	Albizia lebbeck L. High swelling	398:429	Therefore, the current paper presents the studies on structural, functional and drug release study of water soluble polysaccharide (ALPS) from seeds of Albizia lebbeck L. High swelling, water holding capacity, foam stability and lower moisture content suggests its use as additive in food preparations.
28917894	1	50	theme	structural	299:308	arg1	study					339:343	structural, functional and drug release study	299:343	structural, functional and drug release study of water soluble polysaccharide (ALPS) from seeds of Albizia lebbeck L. High swelling, water holding capacity, foam stability and lower moisture content suggests its use as additive in food preparations	299:546	Therefore, the current paper presents the studies on structural, functional and drug release study of water soluble polysaccharide (ALPS) from seeds of Albizia lebbeck L. High swelling, water holding capacity, foam stability and lower moisture content suggests its use as additive in food preparations.
28917894	4	51	theme	Micromeritic	787:798	arg1	properties					800:809	Micromeritic properties	787:809	Micromeritic properties	787:809	Micromeritic properties revealed that the polysaccharide possess potential for pharmaceutical applications.
28917894	1	52	from	use	511:513	arg1	preparations					535:546	food preparations	530:546	food preparations	530:546	Therefore, the current paper presents the studies on structural, functional and drug release study of water soluble polysaccharide (ALPS) from seeds of Albizia lebbeck L. High swelling, water holding capacity, foam stability and lower moisture content suggests its use as additive in food preparations.
28917894	4	53	contain	possess	844:850	arg1	polysaccharide					829:842	the polysaccharide	825:842	the polysaccharide	825:842	Micromeritic properties revealed that the polysaccharide possess potential for pharmaceutical applications.
28917894	4	53	contain	possess	844:850	arg2	potential					852:860	potential	852:860	potential for pharmaceutical applications	852:892	Micromeritic properties revealed that the polysaccharide possess potential for pharmaceutical applications.
28917894	0	54	from	excipients	217:226	arg1	delivery					236:243	drug delivery	231:243	drug delivery	231:243	Structural, functional and pH sensitive release characteristics of water-soluble polysaccharide from the seeds of Albizia lebbeck L. Plant polysaccharides, generally regarded as safe (GRAS), are gaining importance as excipients in drug delivery.
28917894	6	55	with	polysaccharide	1008:1021	arg1	shape					1047:1051	irregular particle shape	1028:1051	irregular particle shape	1028:1051	Morphological study reveals the polysaccharide with irregular particle shape and rough surface.
28917894	6	55	with	polysaccharide	1008:1021	arg1	surface					1063:1069	rough surface	1057:1069	rough surface	1057:1069	Morphological study reveals the polysaccharide with irregular particle shape and rough surface.
28917894	9	56	theme	release	1311:1317	arg1	profile					1319:1325	The drug release profile	1302:1325	The drug release profile	1302:1325	The drug release profile reveals the use of polysaccharide for the preparation of pH sensitive pharmaceutical dosage forms.
28917894	0	57	from	seeds	105:109	arg1	polysaccharide					81:94	water-soluble polysaccharide	67:94	water-soluble polysaccharide from the seeds of Albizia lebbeck L. Plant polysaccharides, generally regarded as safe (GRAS),	67:189	Structural, functional and pH sensitive release characteristics of water-soluble polysaccharide from the seeds of Albizia lebbeck L. Plant polysaccharides, generally regarded as safe (GRAS), are gaining importance as excipients in drug delivery.
28917894	0	57	from	seeds	105:109	arg1	characteristics					48:62	Structural, functional and pH sensitive release characteristics	0:62	Structural, functional and pH sensitive release characteristics of water-soluble polysaccharide from the seeds of Albizia lebbeck L. Plant polysaccharides, generally regarded as safe (GRAS),	0:189	Structural, functional and pH sensitive release characteristics of water-soluble polysaccharide from the seeds of Albizia lebbeck L. Plant polysaccharides, generally regarded as safe (GRAS), are gaining importance as excipients in drug delivery.
28917894	5	58	theme	surface	904:910	arg1	analysis					919:926	the surface charge analysis	900:926	the surface charge analysis	900:926	From the surface charge analysis, ALPS was found to be non-ionic polysaccharide.
28917894	6	59	theme	particle	1038:1045	arg1	shape					1047:1051	irregular particle shape	1028:1051	irregular particle shape	1028:1051	Morphological study reveals the polysaccharide with irregular particle shape and rough surface.
28917894	5	60	theme	non-ionic	950:958	arg1	polysaccharide					960:973	non-ionic polysaccharide	950:973	non-ionic polysaccharide	950:973	From the surface charge analysis, ALPS was found to be non-ionic polysaccharide.
28917894	0	61	theme	water-soluble	67:79	arg1	polysaccharide					81:94	water-soluble polysaccharide	67:94	water-soluble polysaccharide from the seeds of Albizia lebbeck L. Plant polysaccharides, generally regarded as safe (GRAS),	67:189	Structural, functional and pH sensitive release characteristics of water-soluble polysaccharide from the seeds of Albizia lebbeck L. Plant polysaccharides, generally regarded as safe (GRAS), are gaining importance as excipients in drug delivery.
28917894	3	62	theme	Monosaccharide	626:639	arg1	analysis					653:660	Monosaccharide composition analysis	626:660	Monosaccharide composition analysis	626:660	Monosaccharide composition analysis indicated that ALPS consists of mannose (4.06%), rhamnose (22.79%), glucose (38.9%), galactose (17.84%) and xylose (16.42%).
28917894	5	63	theme	charge	912:917	arg1	analysis					919:926	the surface charge analysis	900:926	the surface charge analysis	900:926	From the surface charge analysis, ALPS was found to be non-ionic polysaccharide.
28917894	1	64	theme	lower	475:479	arg1	swelling					422:429	Albizia lebbeck L. High swelling	398:429	Albizia lebbeck L. High swelling	398:429	Therefore, the current paper presents the studies on structural, functional and drug release study of water soluble polysaccharide (ALPS) from seeds of Albizia lebbeck L. High swelling, water holding capacity, foam stability and lower moisture content suggests its use as additive in food preparations.
28917894	1	64	theme	lower	475:479	arg1	content					490:496	lower moisture content	475:496	lower moisture content	475:496	Therefore, the current paper presents the studies on structural, functional and drug release study of water soluble polysaccharide (ALPS) from seeds of Albizia lebbeck L. High swelling, water holding capacity, foam stability and lower moisture content suggests its use as additive in food preparations.
28917894	6	65	theme	irregular	1028:1036	arg1	shape					1047:1051	irregular particle shape	1028:1051	irregular particle shape	1028:1051	Morphological study reveals the polysaccharide with irregular particle shape and rough surface.
28917894	9	66	theme	forms	1419:1423	arg1	preparation					1369:1379	the preparation	1365:1379	the preparation of pH sensitive pharmaceutical dosage forms	1365:1423	The drug release profile reveals the use of polysaccharide for the preparation of pH sensitive pharmaceutical dosage forms.
28917894	4	67	theme	pharmaceutical	866:879	arg1	applications					881:892	pharmaceutical applications	866:892	pharmaceutical applications	866:892	Micromeritic properties revealed that the polysaccharide possess potential for pharmaceutical applications.
28917894	1	68	from	study	339:343	arg1	seeds					389:393	seeds	389:393	seeds of Albizia lebbeck L. High swelling, water holding capacity, foam stability and lower moisture content suggests its use as additive in food preparations	389:546	Therefore, the current paper presents the studies on structural, functional and drug release study of water soluble polysaccharide (ALPS) from seeds of Albizia lebbeck L. High swelling, water holding capacity, foam stability and lower moisture content suggests its use as additive in food preparations.
28917894	1	69	theme	moisture	481:488	arg1	swelling					422:429	Albizia lebbeck L. High swelling	398:429	Albizia lebbeck L. High swelling	398:429	Therefore, the current paper presents the studies on structural, functional and drug release study of water soluble polysaccharide (ALPS) from seeds of Albizia lebbeck L. High swelling, water holding capacity, foam stability and lower moisture content suggests its use as additive in food preparations.
28917894	1	69	theme	moisture	481:488	arg1	content					490:496	lower moisture content	475:496	lower moisture content	475:496	Therefore, the current paper presents the studies on structural, functional and drug release study of water soluble polysaccharide (ALPS) from seeds of Albizia lebbeck L. High swelling, water holding capacity, foam stability and lower moisture content suggests its use as additive in food preparations.
28917894	7	70	theme	spectroscopy	1101:1112	arg1	study					1121:1125	Fourier transformed infrared spectroscopy (FTIR) study	1072:1125	Fourier transformed infrared spectroscopy (FTIR) study	1072:1125	Fourier transformed infrared spectroscopy (FTIR) study confirms the carbohydrate nature of polysaccharide.
26879913	10	0	theme	Na	1393:1394	arg1	-ATPase					1403:1409	Na(+)/K(+)-ATPase	1393:1409	Na(+)/K(+)-ATPase activity	1393:1418	In conclusion, PLS exerts anti-diarrheal activity by increasing Na(+)/K(+)-ATPase activity, inhibiting gastrointestinal motility, and blocking the toxin-GM1 receptor binding.
26879913	6	1	theme	severity	842:849	arg1	stools					826:831	diarrheal stools	816:831	diarrheal stools	816:831	Pretreatment with all doses of PLS inhibited castor oil-induced diarrhea, with reduction of the total amount of stool, diarrheal stools, and the severity of diarrhea.
26879913	6	1	theme	severity	842:849	arg1	amount					799:804	the total amount	789:804	the total amount of stool, diarrheal stools, and the severity of diarrhea	789:861	Pretreatment with all doses of PLS inhibited castor oil-induced diarrhea, with reduction of the total amount of stool, diarrheal stools, and the severity of diarrhea.
26879913	6	1	theme	severity	842:849	arg1	severity					842:849	the severity	838:849	the severity of diarrhea	838:861	Pretreatment with all doses of PLS inhibited castor oil-induced diarrhea, with reduction of the total amount of stool, diarrheal stools, and the severity of diarrhea.
26879913	6	1	theme	severity	842:849	arg1	stool					809:813	stool	809:813	stool	809:813	Pretreatment with all doses of PLS inhibited castor oil-induced diarrhea, with reduction of the total amount of stool, diarrheal stools, and the severity of diarrhea.
26879913	5	2	theme	sulfate	608:614	arg1	content					616:622	The sulfate content	604:622	The sulfate content of the polysaccharide	604:644	The sulfate content of the polysaccharide was 5.08% and the percentage of carbon was 25.98%.
26879913	5	2	theme	sulfate	608:614	arg1	%					654:654	5.08%	650:654	5.08%	650:654	The sulfate content of the polysaccharide was 5.08% and the percentage of carbon was 25.98%.
26879913	2	3	theme	seaweed	352:358	arg1	musciformis					367:377	the red seaweed Hypnea musciformis	344:377	the red seaweed Hypnea musciformis in different animal models	344:404	This study was designed to evaluate the chemical composition and anti-diarrheal activity of a fraction of sulphated polysaccharide (PLS) obtained from the red seaweed Hypnea musciformis in different animal models, and to elucidate the underlying mechanisms.
26879913	4	4	theme	carbohydrate	550:561	arg1	content					563:569	The total carbohydrate content	540:569	The total carbohydrate content	540:569	The total carbohydrate content accounted for 99% of the sample.
26879913	0	5	from	efficacy	4:11	arg1	musciformis					64:74	Hypnea musciformis	57:74	Hypnea musciformis	57:74	The efficacy of a sulphated polysaccharide fraction from Hypnea musciformis against diarrhea in rodents.
26879913	2	6	theme	red	348:350	arg1	musciformis					367:377	the red seaweed Hypnea musciformis	344:377	the red seaweed Hypnea musciformis in different animal models	344:404	This study was designed to evaluate the chemical composition and anti-diarrheal activity of a fraction of sulphated polysaccharide (PLS) obtained from the red seaweed Hypnea musciformis in different animal models, and to elucidate the underlying mechanisms.
26879913	8	7	from	activity	993:1000	arg1	intestine					1015:1023	the small intestine	1005:1023	the small intestine	1005:1023	In addition, PLS (90 mg/Kg) increased the Na(+)/K(+)-ATPase activity in the small intestine and reduced gastrointestinal transit, possibly via activation of cholinergic receptors.
26879913	4	8	theme	total	544:548	arg1	content					563:569	The total carbohydrate content	540:569	The total carbohydrate content	540:569	The total carbohydrate content accounted for 99% of the sample.
26879913	5	9	theme	carbon	678:683	arg1	%					694:694	25.98%	689:694	25.98%	689:694	The sulfate content of the polysaccharide was 5.08% and the percentage of carbon was 25.98%.
26879913	5	9	theme	carbon	678:683	arg1	percentage					664:673	the percentage	660:673	the percentage of carbon	660:683	The sulfate content of the polysaccharide was 5.08% and the percentage of carbon was 25.98%.
26879913	3	10	theme	weight	532:537	arg1	weight					532:537	the seaweed dry weight	516:537	the seaweed dry weight	516:537	PLS was obtained by aqueous extraction, with a yield of 31.8% of the seaweed dry weight.
26879913	3	10	theme	weight	532:537	arg1	%					511:511	31.8%	507:511	31.8% of the seaweed dry weight	507:537	PLS was obtained by aqueous extraction, with a yield of 31.8% of the seaweed dry weight.
26879913	8	11	theme	small	1009:1013	arg1	intestine					1015:1023	the small intestine	1005:1023	the small intestine	1005:1023	In addition, PLS (90 mg/Kg) increased the Na(+)/K(+)-ATPase activity in the small intestine and reduced gastrointestinal transit, possibly via activation of cholinergic receptors.
26879913	3	12	theme	seaweed	520:526	arg1	weight					532:537	the seaweed dry weight	516:537	the seaweed dry weight	516:537	PLS was obtained by aqueous extraction, with a yield of 31.8% of the seaweed dry weight.
26879913	6	13	theme	oil-induced	749:759	arg1	diarrhea					761:768	castor oil-induced diarrhea	742:768	castor oil-induced diarrhea	742:768	Pretreatment with all doses of PLS inhibited castor oil-induced diarrhea, with reduction of the total amount of stool, diarrheal stools, and the severity of diarrhea.
26879913	2	14	theme	fraction	287:294	arg1	composition					242:252	chemical composition	233:252	chemical composition	233:252	This study was designed to evaluate the chemical composition and anti-diarrheal activity of a fraction of sulphated polysaccharide (PLS) obtained from the red seaweed Hypnea musciformis in different animal models, and to elucidate the underlying mechanisms.
26879913	2	14	theme	fraction	287:294	arg1	activity					273:280	anti-diarrheal activity	258:280	anti-diarrheal activity	258:280	This study was designed to evaluate the chemical composition and anti-diarrheal activity of a fraction of sulphated polysaccharide (PLS) obtained from the red seaweed Hypnea musciformis in different animal models, and to elucidate the underlying mechanisms.
26879913	8	15	theme	-ATPase	985:991	arg1	activity					993:1000	the Na(+)/K(+)-ATPase activity	971:1000	the Na(+)/K(+)-ATPase activity in the small intestine	971:1023	In addition, PLS (90 mg/Kg) increased the Na(+)/K(+)-ATPase activity in the small intestine and reduced gastrointestinal transit, possibly via activation of cholinergic receptors.
26879913	10	16	theme	/K	1398:1399	arg1	-ATPase					1403:1409	Na(+)/K(+)-ATPase	1393:1409	Na(+)/K(+)-ATPase activity	1393:1418	In conclusion, PLS exerts anti-diarrheal activity by increasing Na(+)/K(+)-ATPase activity, inhibiting gastrointestinal motility, and blocking the toxin-GM1 receptor binding.
26879913	6	17	theme	stools	826:831	arg1	stools					826:831	diarrheal stools	816:831	diarrheal stools	816:831	Pretreatment with all doses of PLS inhibited castor oil-induced diarrhea, with reduction of the total amount of stool, diarrheal stools, and the severity of diarrhea.
26879913	6	17	theme	stools	826:831	arg1	amount					799:804	the total amount	789:804	the total amount of stool, diarrheal stools, and the severity of diarrhea	789:861	Pretreatment with all doses of PLS inhibited castor oil-induced diarrhea, with reduction of the total amount of stool, diarrheal stools, and the severity of diarrhea.
26879913	6	17	theme	stools	826:831	arg1	severity					842:849	the severity	838:849	the severity of diarrhea	838:861	Pretreatment with all doses of PLS inhibited castor oil-induced diarrhea, with reduction of the total amount of stool, diarrheal stools, and the severity of diarrhea.
26879913	6	17	theme	stools	826:831	arg1	stool					809:813	stool	809:813	stool	809:813	Pretreatment with all doses of PLS inhibited castor oil-induced diarrhea, with reduction of the total amount of stool, diarrheal stools, and the severity of diarrhea.
26879913	6	18	theme	castor	742:747	arg1	diarrhea					761:768	castor oil-induced diarrhea	742:768	castor oil-induced diarrhea	742:768	Pretreatment with all doses of PLS inhibited castor oil-induced diarrhea, with reduction of the total amount of stool, diarrheal stools, and the severity of diarrhea.
26879913	3	19	theme	%	511:511	arg1	yield					498:502	a yield	496:502	a yield of 31.8% of the seaweed dry weight	496:537	PLS was obtained by aqueous extraction, with a yield of 31.8% of the seaweed dry weight.
26879913	10	20	theme	toxin-GM1	1476:1484	arg1	binding					1495:1501	the toxin-GM1 receptor binding	1472:1501	the toxin-GM1 receptor binding	1472:1501	In conclusion, PLS exerts anti-diarrheal activity by increasing Na(+)/K(+)-ATPase activity, inhibiting gastrointestinal motility, and blocking the toxin-GM1 receptor binding.
26879913	9	21	theme	toxin-GM1	1301:1309	arg1	binding					1320:1326	toxin-GM1 receptor binding	1301:1326	toxin-GM1 receptor binding	1301:1326	Interestingly, the cholera toxin-induced fluid secretion and Cl(-) ion levels decreased in the intestinal contents of the animals pretreated with PLS (90 mg/kg), probably via reduction of toxin-GM1 receptor binding.
26879913	6	22	theme	diarrheal	816:824	arg1	stools					826:831	diarrheal stools	816:831	diarrheal stools	816:831	Pretreatment with all doses of PLS inhibited castor oil-induced diarrhea, with reduction of the total amount of stool, diarrheal stools, and the severity of diarrhea.
26879913	1	23	theme	sulphated	166:174	arg1	polysaccharides					176:190	sulphated polysaccharides	166:190	sulphated polysaccharides	166:190	Seaweeds are sources of diverse bioactive compounds, such as sulphated polysaccharides.
26879913	6	24	theme	PLS	728:730	arg1	doses					719:723	all doses	715:723	all doses of PLS	715:730	Pretreatment with all doses of PLS inhibited castor oil-induced diarrhea, with reduction of the total amount of stool, diarrheal stools, and the severity of diarrhea.
26879913	2	25	theme	PLS	325:327	arg1	fraction					287:294	a fraction	285:294	a fraction of sulphated polysaccharide (PLS) obtained from the red seaweed Hypnea musciformis in different animal models	285:404	This study was designed to evaluate the chemical composition and anti-diarrheal activity of a fraction of sulphated polysaccharide (PLS) obtained from the red seaweed Hypnea musciformis in different animal models, and to elucidate the underlying mechanisms.
26879913	2	26	theme	different	382:390	arg1	models					399:404	different animal models	382:404	different animal models	382:404	This study was designed to evaluate the chemical composition and anti-diarrheal activity of a fraction of sulphated polysaccharide (PLS) obtained from the red seaweed Hypnea musciformis in different animal models, and to elucidate the underlying mechanisms.
26879913	8	27	theme	gastrointestinal	1037:1052	arg1	transit					1054:1060	gastrointestinal transit	1037:1060	gastrointestinal transit	1037:1060	In addition, PLS (90 mg/Kg) increased the Na(+)/K(+)-ATPase activity in the small intestine and reduced gastrointestinal transit, possibly via activation of cholinergic receptors.
26879913	2	28	from	musciformis	367:377	arg1	models					399:404	different animal models	382:404	different animal models	382:404	This study was designed to evaluate the chemical composition and anti-diarrheal activity of a fraction of sulphated polysaccharide (PLS) obtained from the red seaweed Hypnea musciformis in different animal models, and to elucidate the underlying mechanisms.
26879913	2	29	theme	underlying	428:437	arg1	mechanisms					439:448	the underlying mechanisms	424:448	the underlying mechanisms	424:448	This study was designed to evaluate the chemical composition and anti-diarrheal activity of a fraction of sulphated polysaccharide (PLS) obtained from the red seaweed Hypnea musciformis in different animal models, and to elucidate the underlying mechanisms.
26879913	2	30	theme	polysaccharide	309:322	arg1	PLS					325:327	sulphated polysaccharide (PLS)	299:328	sulphated polysaccharide (PLS) obtained from the red seaweed Hypnea musciformis in different animal models	299:404	This study was designed to evaluate the chemical composition and anti-diarrheal activity of a fraction of sulphated polysaccharide (PLS) obtained from the red seaweed Hypnea musciformis in different animal models, and to elucidate the underlying mechanisms.
26879913	2	31	dep	composition	242:252	arg1	the					229:231	the	229:231	the	229:231	This study was designed to evaluate the chemical composition and anti-diarrheal activity of a fraction of sulphated polysaccharide (PLS) obtained from the red seaweed Hypnea musciformis in different animal models, and to elucidate the underlying mechanisms.
26879913	4	32	theme	sample	596:601	arg1	%					587:587	99%	585:587	99% of the sample	585:601	The total carbohydrate content accounted for 99% of the sample.
26879913	4	32	theme	sample	596:601	arg1	sample					596:601	the sample	592:601	the sample	592:601	The total carbohydrate content accounted for 99% of the sample.
26879913	8	33	theme	Na	975:976	arg1	activity					993:1000	the Na(+)/K(+)-ATPase activity	971:1000	the Na(+)/K(+)-ATPase activity in the small intestine	971:1023	In addition, PLS (90 mg/Kg) increased the Na(+)/K(+)-ATPase activity in the small intestine and reduced gastrointestinal transit, possibly via activation of cholinergic receptors.
26879913	0	34	from	diarrhea	84:91	arg1	rodents					96:102	rodents	96:102	rodents	96:102	The efficacy of a sulphated polysaccharide fraction from Hypnea musciformis against diarrhea in rodents.
26879913	2	35	theme	sulphated	299:307	arg1	PLS					325:327	sulphated polysaccharide (PLS)	299:328	sulphated polysaccharide (PLS) obtained from the red seaweed Hypnea musciformis in different animal models	299:404	This study was designed to evaluate the chemical composition and anti-diarrheal activity of a fraction of sulphated polysaccharide (PLS) obtained from the red seaweed Hypnea musciformis in different animal models, and to elucidate the underlying mechanisms.
26879913	9	36	theme	receptor	1311:1318	arg1	binding					1320:1326	toxin-GM1 receptor binding	1301:1326	toxin-GM1 receptor binding	1301:1326	Interestingly, the cholera toxin-induced fluid secretion and Cl(-) ion levels decreased in the intestinal contents of the animals pretreated with PLS (90 mg/kg), probably via reduction of toxin-GM1 receptor binding.
26879913	0	37	theme	polysaccharide	28:41	arg1	fraction					43:50	a sulphated polysaccharide fraction	16:50	a sulphated polysaccharide fraction from Hypnea musciformis	16:74	The efficacy of a sulphated polysaccharide fraction from Hypnea musciformis against diarrhea in rodents.
26879913	9	38	theme	intestinal	1208:1217	arg1	contents					1219:1226	the intestinal contents	1204:1226	the intestinal contents of the animals pretreated with PLS (90 mg/kg)	1204:1272	Interestingly, the cholera toxin-induced fluid secretion and Cl(-) ion levels decreased in the intestinal contents of the animals pretreated with PLS (90 mg/kg), probably via reduction of toxin-GM1 receptor binding.
26879913	2	39	theme	chemical	233:240	arg1	composition					242:252	chemical composition	233:252	chemical composition	233:252	This study was designed to evaluate the chemical composition and anti-diarrheal activity of a fraction of sulphated polysaccharide (PLS) obtained from the red seaweed Hypnea musciformis in different animal models, and to elucidate the underlying mechanisms.
26879913	9	40	theme	binding	1320:1326	arg1	reduction					1288:1296	reduction	1288:1296	reduction of toxin-GM1 receptor binding	1288:1326	Interestingly, the cholera toxin-induced fluid secretion and Cl(-) ion levels decreased in the intestinal contents of the animals pretreated with PLS (90 mg/kg), probably via reduction of toxin-GM1 receptor binding.
26879913	0	41	theme	sulphated	18:26	arg1	fraction					43:50	a sulphated polysaccharide fraction	16:50	a sulphated polysaccharide fraction from Hypnea musciformis	16:74	The efficacy of a sulphated polysaccharide fraction from Hypnea musciformis against diarrhea in rodents.
26879913	0	42	from	musciformis	64:74	arg1	fraction					43:50	a sulphated polysaccharide fraction	16:50	a sulphated polysaccharide fraction from Hypnea musciformis	16:74	The efficacy of a sulphated polysaccharide fraction from Hypnea musciformis against diarrhea in rodents.
26879913	0	42	from	musciformis	64:74	arg1	efficacy					4:11	The efficacy	0:11	The efficacy of a sulphated polysaccharide fraction from Hypnea musciformis against diarrhea in rodents.	0:103	The efficacy of a sulphated polysaccharide fraction from Hypnea musciformis against diarrhea in rodents.
26879913	10	43	theme	-ATPase	1403:1409	arg1	activity					1411:1418	Na(+)/K(+)-ATPase activity	1393:1418	Na(+)/K(+)-ATPase activity	1393:1418	In conclusion, PLS exerts anti-diarrheal activity by increasing Na(+)/K(+)-ATPase activity, inhibiting gastrointestinal motility, and blocking the toxin-GM1 receptor binding.
26879913	9	44	theme	animals	1235:1241	arg1	contents					1219:1226	the intestinal contents	1204:1226	the intestinal contents of the animals pretreated with PLS (90 mg/kg)	1204:1272	Interestingly, the cholera toxin-induced fluid secretion and Cl(-) ion levels decreased in the intestinal contents of the animals pretreated with PLS (90 mg/kg), probably via reduction of toxin-GM1 receptor binding.
26879913	8	45	theme	cholinergic	1090:1100	arg1	receptors					1102:1110	cholinergic receptors	1090:1110	cholinergic receptors	1090:1110	In addition, PLS (90 mg/Kg) increased the Na(+)/K(+)-ATPase activity in the small intestine and reduced gastrointestinal transit, possibly via activation of cholinergic receptors.
26879913	5	46	theme	polysaccharide	631:644	arg1	content					616:622	The sulfate content	604:622	The sulfate content of the polysaccharide	604:644	The sulfate content of the polysaccharide was 5.08% and the percentage of carbon was 25.98%.
26879913	5	46	theme	polysaccharide	631:644	arg1	%					654:654	5.08%	650:654	5.08%	650:654	The sulfate content of the polysaccharide was 5.08% and the percentage of carbon was 25.98%.
26879913	6	47	theme	total	793:797	arg1	stools					826:831	diarrheal stools	816:831	diarrheal stools	816:831	Pretreatment with all doses of PLS inhibited castor oil-induced diarrhea, with reduction of the total amount of stool, diarrheal stools, and the severity of diarrhea.
26879913	6	47	theme	total	793:797	arg1	amount					799:804	the total amount	789:804	the total amount of stool, diarrheal stools, and the severity of diarrhea	789:861	Pretreatment with all doses of PLS inhibited castor oil-induced diarrhea, with reduction of the total amount of stool, diarrheal stools, and the severity of diarrhea.
26879913	6	47	theme	total	793:797	arg1	severity					842:849	the severity	838:849	the severity of diarrhea	838:861	Pretreatment with all doses of PLS inhibited castor oil-induced diarrhea, with reduction of the total amount of stool, diarrheal stools, and the severity of diarrhea.
26879913	6	47	theme	total	793:797	arg1	stool					809:813	stool	809:813	stool	809:813	Pretreatment with all doses of PLS inhibited castor oil-induced diarrhea, with reduction of the total amount of stool, diarrheal stools, and the severity of diarrhea.
26879913	6	48	theme	stool	809:813	arg1	stools					826:831	diarrheal stools	816:831	diarrheal stools	816:831	Pretreatment with all doses of PLS inhibited castor oil-induced diarrhea, with reduction of the total amount of stool, diarrheal stools, and the severity of diarrhea.
26879913	6	48	theme	stool	809:813	arg1	amount					799:804	the total amount	789:804	the total amount of stool, diarrheal stools, and the severity of diarrhea	789:861	Pretreatment with all doses of PLS inhibited castor oil-induced diarrhea, with reduction of the total amount of stool, diarrheal stools, and the severity of diarrhea.
26879913	6	48	theme	stool	809:813	arg1	severity					842:849	the severity	838:849	the severity of diarrhea	838:861	Pretreatment with all doses of PLS inhibited castor oil-induced diarrhea, with reduction of the total amount of stool, diarrheal stools, and the severity of diarrhea.
26879913	6	48	theme	stool	809:813	arg1	stool					809:813	stool	809:813	stool	809:813	Pretreatment with all doses of PLS inhibited castor oil-induced diarrhea, with reduction of the total amount of stool, diarrheal stools, and the severity of diarrhea.
26879913	9	49	theme	ion	1180:1182	arg1	levels					1184:1189	Cl(-) ion levels	1174:1189	Cl(-) ion levels	1174:1189	Interestingly, the cholera toxin-induced fluid secretion and Cl(-) ion levels decreased in the intestinal contents of the animals pretreated with PLS (90 mg/kg), probably via reduction of toxin-GM1 receptor binding.
26879913	0	50	theme	fraction	43:50	arg1	efficacy					4:11	The efficacy	0:11	The efficacy of a sulphated polysaccharide fraction from Hypnea musciformis against diarrhea in rodents.	0:103	The efficacy of a sulphated polysaccharide fraction from Hypnea musciformis against diarrhea in rodents.
26879913	8	51	theme	/K	980:981	arg1	activity					993:1000	the Na(+)/K(+)-ATPase activity	971:1000	the Na(+)/K(+)-ATPase activity in the small intestine	971:1023	In addition, PLS (90 mg/Kg) increased the Na(+)/K(+)-ATPase activity in the small intestine and reduced gastrointestinal transit, possibly via activation of cholinergic receptors.
26879913	1	52	theme	compounds	147:155	arg1	sources					118:124	sources	118:124	sources of diverse bioactive compounds, such as sulphated polysaccharides	118:190	Seaweeds are sources of diverse bioactive compounds, such as sulphated polysaccharides.
26879913	1	52	theme	compounds	147:155	arg1	Seaweeds					105:112	Seaweeds	105:112	Seaweeds	105:112	Seaweeds are sources of diverse bioactive compounds, such as sulphated polysaccharides.
26879913	10	53	theme	gastrointestinal	1432:1447	arg1	motility					1449:1456	gastrointestinal motility	1432:1456	gastrointestinal motility	1432:1456	In conclusion, PLS exerts anti-diarrheal activity by increasing Na(+)/K(+)-ATPase activity, inhibiting gastrointestinal motility, and blocking the toxin-GM1 receptor binding.
26879913	6	54	theme	amount	799:804	arg1	reduction					776:784	reduction	776:784	reduction of the total amount of stool, diarrheal stools, and the severity of diarrhea	776:861	Pretreatment with all doses of PLS inhibited castor oil-induced diarrhea, with reduction of the total amount of stool, diarrheal stools, and the severity of diarrhea.
26879913	6	55	theme	diarrhea	854:861	arg1	stools					826:831	diarrheal stools	816:831	diarrheal stools	816:831	Pretreatment with all doses of PLS inhibited castor oil-induced diarrhea, with reduction of the total amount of stool, diarrheal stools, and the severity of diarrhea.
26879913	6	55	theme	diarrhea	854:861	arg1	severity					842:849	the severity	838:849	the severity of diarrhea	838:861	Pretreatment with all doses of PLS inhibited castor oil-induced diarrhea, with reduction of the total amount of stool, diarrheal stools, and the severity of diarrhea.
26879913	6	55	theme	diarrhea	854:861	arg1	stool					809:813	stool	809:813	stool	809:813	Pretreatment with all doses of PLS inhibited castor oil-induced diarrhea, with reduction of the total amount of stool, diarrheal stools, and the severity of diarrhea.
26879913	10	56	theme	anti-diarrheal	1355:1368	arg1	activity					1370:1377	anti-diarrheal activity	1355:1377	anti-diarrheal activity	1355:1377	In conclusion, PLS exerts anti-diarrheal activity by increasing Na(+)/K(+)-ATPase activity, inhibiting gastrointestinal motility, and blocking the toxin-GM1 receptor binding.
26879913	9	57	theme	cholera	1132:1138	arg1	secretion					1160:1168	the cholera toxin-induced fluid secretion	1128:1168	the cholera toxin-induced fluid secretion	1128:1168	Interestingly, the cholera toxin-induced fluid secretion and Cl(-) ion levels decreased in the intestinal contents of the animals pretreated with PLS (90 mg/kg), probably via reduction of toxin-GM1 receptor binding.
26879913	3	58	theme	aqueous	471:477	arg1	extraction					479:488	aqueous extraction	471:488	aqueous extraction	471:488	PLS was obtained by aqueous extraction, with a yield of 31.8% of the seaweed dry weight.
26879913	3	59	theme	dry	528:530	arg1	weight					532:537	the seaweed dry weight	516:537	the seaweed dry weight	516:537	PLS was obtained by aqueous extraction, with a yield of 31.8% of the seaweed dry weight.
26879913	9	60	theme	toxin-induced	1140:1152	arg1	secretion					1160:1168	the cholera toxin-induced fluid secretion	1128:1168	the cholera toxin-induced fluid secretion	1128:1168	Interestingly, the cholera toxin-induced fluid secretion and Cl(-) ion levels decreased in the intestinal contents of the animals pretreated with PLS (90 mg/kg), probably via reduction of toxin-GM1 receptor binding.
26879913	2	61	theme	anti-diarrheal	258:271	arg1	activity					273:280	anti-diarrheal activity	258:280	anti-diarrheal activity	258:280	This study was designed to evaluate the chemical composition and anti-diarrheal activity of a fraction of sulphated polysaccharide (PLS) obtained from the red seaweed Hypnea musciformis in different animal models, and to elucidate the underlying mechanisms.
26879913	10	62	theme	receptor	1486:1493	arg1	binding					1495:1501	the toxin-GM1 receptor binding	1472:1501	the toxin-GM1 receptor binding	1472:1501	In conclusion, PLS exerts anti-diarrheal activity by increasing Na(+)/K(+)-ATPase activity, inhibiting gastrointestinal motility, and blocking the toxin-GM1 receptor binding.
26879913	1	63	theme	diverse	129:135	arg1	polysaccharides					176:190	sulphated polysaccharides	166:190	sulphated polysaccharides	166:190	Seaweeds are sources of diverse bioactive compounds, such as sulphated polysaccharides.
26879913	1	63	theme	diverse	129:135	arg1	compounds					147:155	diverse bioactive compounds	129:155	diverse bioactive compounds	129:155	Seaweeds are sources of diverse bioactive compounds, such as sulphated polysaccharides.
26879913	9	64	theme	Cl	1174:1175	arg1	levels					1184:1189	Cl(-) ion levels	1174:1189	Cl(-) ion levels	1174:1189	Interestingly, the cholera toxin-induced fluid secretion and Cl(-) ion levels decreased in the intestinal contents of the animals pretreated with PLS (90 mg/kg), probably via reduction of toxin-GM1 receptor binding.
26879913	6	65	with	Pretreatment	697:708	arg1	doses					719:723	all doses	715:723	all doses of PLS	715:730	Pretreatment with all doses of PLS inhibited castor oil-induced diarrhea, with reduction of the total amount of stool, diarrheal stools, and the severity of diarrhea.
26879913	9	66	theme	fluid	1154:1158	arg1	secretion					1160:1168	the cholera toxin-induced fluid secretion	1128:1168	the cholera toxin-induced fluid secretion	1128:1168	Interestingly, the cholera toxin-induced fluid secretion and Cl(-) ion levels decreased in the intestinal contents of the animals pretreated with PLS (90 mg/kg), probably via reduction of toxin-GM1 receptor binding.
26879913	7	67	theme	PGE2-induced	905:916	arg1	enteropooling					918:930	PGE2-induced enteropooling	905:930	PGE2-induced enteropooling	905:930	PLS (90 mg/Kg) decreased castor oil- and PGE2-induced enteropooling.
26879913	1	68	theme	bioactive	137:145	arg1	polysaccharides					176:190	sulphated polysaccharides	166:190	sulphated polysaccharides	166:190	Seaweeds are sources of diverse bioactive compounds, such as sulphated polysaccharides.
26879913	1	68	theme	bioactive	137:145	arg1	compounds					147:155	diverse bioactive compounds	129:155	diverse bioactive compounds	129:155	Seaweeds are sources of diverse bioactive compounds, such as sulphated polysaccharides.
26879913	2	69	theme	animal	392:397	arg1	models					399:404	different animal models	382:404	different animal models	382:404	This study was designed to evaluate the chemical composition and anti-diarrheal activity of a fraction of sulphated polysaccharide (PLS) obtained from the red seaweed Hypnea musciformis in different animal models, and to elucidate the underlying mechanisms.
26879913	0	70	theme	Hypnea	57:62	arg1	musciformis					64:74	Hypnea musciformis	57:74	Hypnea musciformis	57:74	The efficacy of a sulphated polysaccharide fraction from Hypnea musciformis against diarrhea in rodents.
26879913	8	71	theme	receptors	1102:1110	arg1	activation					1076:1085	activation	1076:1085	activation of cholinergic receptors	1076:1110	In addition, PLS (90 mg/Kg) increased the Na(+)/K(+)-ATPase activity in the small intestine and reduced gastrointestinal transit, possibly via activation of cholinergic receptors.
26879913	7	72	theme	castor	889:894	arg1	oil-					896:899	castor oil-	889:899	castor oil-	889:899	PLS (90 mg/Kg) decreased castor oil- and PGE2-induced enteropooling.
26879913	2	73	theme	Hypnea	360:365	arg1	musciformis					367:377	the red seaweed Hypnea musciformis	344:377	the red seaweed Hypnea musciformis in different animal models	344:404	This study was designed to evaluate the chemical composition and anti-diarrheal activity of a fraction of sulphated polysaccharide (PLS) obtained from the red seaweed Hypnea musciformis in different animal models, and to elucidate the underlying mechanisms.
24752927	0	0	theme	compressed	63:72	arg1	water					74:78	hot compressed water pretreatment and enhanced enzymatic hydrolysis	59:125	water	74:78	Changes in plant cell-wall structure of corn stover due to hot compressed water pretreatment and enhanced enzymatic hydrolysis.
24752927	6	1	from	°C	963:964	arg1	pretreatment					934:945	optimized pretreatment	924:945	optimized pretreatment with HCW at 185 °C	924:964	Enzyme saccharification of solids by optimized pretreatment with HCW at 185 °C resulted in an enzymatic hydrolysis yield of 87 %, an enhancement of 77 % compared to the yield from untreated corn stover.
24752927	6	2	theme	%	1014:1014	arg1	yield					1002:1006	an enzymatic hydrolysis yield	978:1006	an enzymatic hydrolysis yield of 87 %	978:1014	Enzyme saccharification of solids by optimized pretreatment with HCW at 185 °C resulted in an enzymatic hydrolysis yield of 87 %, an enhancement of 77 % compared to the yield from untreated corn stover.
24752927	0	3	theme	hot	59:61	arg1	water					74:78	hot compressed water pretreatment and enhanced enzymatic hydrolysis	59:125	water	74:78	Changes in plant cell-wall structure of corn stover due to hot compressed water pretreatment and enhanced enzymatic hydrolysis.
24752927	1	4	theme	potential	145:153	arg1	feedstock					155:163	a potential feedstock	143:163	a potential feedstock for biofuel production	143:186	Corn stover is a potential feedstock for biofuel production.
24752927	1	4	theme	potential	145:153	arg1	stover					133:138	Corn stover	128:138	Corn stover	128:138	Corn stover is a potential feedstock for biofuel production.
24752927	3	5	from	%	535:535	arg1	solids					551:556	pretreated solids	540:556	pretreated solids	540:556	Chemical composition analysis showed the soluble hemicellulose content increased with pretreatment temperature, whereas the hemicellulose content decreased from 29 to 7 % in pretreated solids.
24752927	4	6	theme	electron	568:575	arg1	microscopy					577:586	Scanning electron microscopy	559:586	Scanning electron microscopy	559:586	Scanning electron microscopy revealed the parenchyma-type second cell-wall structure of the plant was almost completely removed at 185 °C, and the sclerenchyma-type second cell wall was greatly damaged upon addition of 5 mmol/L ammonium sulfate during HCW pretreatment.
24752927	0	7	from	Changes	0:6	arg1	structure					27:35	plant cell-wall structure	11:35	plant cell-wall structure of corn	11:43	Changes in plant cell-wall structure of corn stover due to hot compressed water pretreatment and enhanced enzymatic hydrolysis.
24752927	2	8	theme	due	286:288	arg1	stover					279:284	corn stover	274:284	corn stover due to hot compressed water (HCW) pretreatment at 170-190 °C in a tube reactor	274:363	This work investigated physical and chemical changes in plant cell-wall structure of corn stover due to hot compressed water (HCW) pretreatment at 170-190 °C in a tube reactor.
24752927	2	9	theme	stover	279:284	arg1	structure					261:269	plant cell-wall structure	245:269	plant cell-wall structure of corn stover due to hot compressed water (HCW) pretreatment at 170-190 °C in a tube reactor	245:363	This work investigated physical and chemical changes in plant cell-wall structure of corn stover due to hot compressed water (HCW) pretreatment at 170-190 °C in a tube reactor.
24752927	4	10	theme	second	724:729	arg1	wall					736:739	the sclerenchyma-type second cell wall	702:739	the sclerenchyma-type second cell wall	702:739	Scanning electron microscopy revealed the parenchyma-type second cell-wall structure of the plant was almost completely removed at 185 °C, and the sclerenchyma-type second cell wall was greatly damaged upon addition of 5 mmol/L ammonium sulfate during HCW pretreatment.
24752927	0	11	theme	enhanced	97:104	arg1	hydrolysis					116:125	enhanced enzymatic hydrolysis	97:125	enhanced enzymatic hydrolysis	97:125	Changes in plant cell-wall structure of corn stover due to hot compressed water pretreatment and enhanced enzymatic hydrolysis.
24752927	6	12	theme	solids	914:919	arg1	saccharification					894:909	Enzyme saccharification	887:909	Enzyme saccharification of solids by optimized pretreatment with HCW at 185 °C	887:964	Enzyme saccharification of solids by optimized pretreatment with HCW at 185 °C resulted in an enzymatic hydrolysis yield of 87 %, an enhancement of 77 % compared to the yield from untreated corn stover.
24752927	6	13	theme	untreated	1067:1075	arg1	stover					1082:1087	untreated corn stover	1067:1087	untreated corn stover	1067:1087	Enzyme saccharification of solids by optimized pretreatment with HCW at 185 °C resulted in an enzymatic hydrolysis yield of 87 %, an enhancement of 77 % compared to the yield from untreated corn stover.
24752927	2	14	theme	corn	274:277	arg1	stover					279:284	corn stover	274:284	corn stover due to hot compressed water (HCW) pretreatment at 170-190 °C in a tube reactor	274:363	This work investigated physical and chemical changes in plant cell-wall structure of corn stover due to hot compressed water (HCW) pretreatment at 170-190 °C in a tube reactor.
24752927	2	15	theme	tube	352:355	arg1	reactor					357:363	a tube reactor	350:363	a tube reactor	350:363	This work investigated physical and chemical changes in plant cell-wall structure of corn stover due to hot compressed water (HCW) pretreatment at 170-190 °C in a tube reactor.
24752927	3	16	theme	hemicellulose	415:427	arg1	content					429:435	the soluble hemicellulose content	403:435	the soluble hemicellulose content	403:435	Chemical composition analysis showed the soluble hemicellulose content increased with pretreatment temperature, whereas the hemicellulose content decreased from 29 to 7 % in pretreated solids.
24752927	3	17	theme	composition	375:385	arg1	analysis					387:394	Chemical composition analysis	366:394	Chemical composition analysis	366:394	Chemical composition analysis showed the soluble hemicellulose content increased with pretreatment temperature, whereas the hemicellulose content decreased from 29 to 7 % in pretreated solids.
24752927	0	18	dep	water	74:78	arg1	pretreatment					80:91	pretreatment	80:91	pretreatment	80:91	Changes in plant cell-wall structure of corn stover due to hot compressed water pretreatment and enhanced enzymatic hydrolysis.
24752927	2	19	from	changes	234:240	arg1	structure					261:269	plant cell-wall structure	245:269	plant cell-wall structure of corn stover due to hot compressed water (HCW) pretreatment at 170-190 °C in a tube reactor	245:363	This work investigated physical and chemical changes in plant cell-wall structure of corn stover due to hot compressed water (HCW) pretreatment at 170-190 °C in a tube reactor.
24752927	4	20	theme	mmol/L	780:785	arg1	sulfate					796:802	5 mmol/L ammonium sulfate	778:802	5 mmol/L ammonium sulfate	778:802	Scanning electron microscopy revealed the parenchyma-type second cell-wall structure of the plant was almost completely removed at 185 °C, and the sclerenchyma-type second cell wall was greatly damaged upon addition of 5 mmol/L ammonium sulfate during HCW pretreatment.
24752927	2	21	theme	water	308:312	arg1	pretreatment					320:331	hot compressed water (HCW) pretreatment	293:331	hot compressed water (HCW) pretreatment at 170-190 °C in a tube reactor	293:363	This work investigated physical and chemical changes in plant cell-wall structure of corn stover due to hot compressed water (HCW) pretreatment at 170-190 °C in a tube reactor.
24752927	1	22	theme	biofuel	169:175	arg1	production					177:186	biofuel production	169:186	biofuel production	169:186	Corn stover is a potential feedstock for biofuel production.
24752927	6	23	theme	hydrolysis	991:1000	arg1	yield					1002:1006	an enzymatic hydrolysis yield	978:1006	an enzymatic hydrolysis yield of 87 %	978:1014	Enzyme saccharification of solids by optimized pretreatment with HCW at 185 °C resulted in an enzymatic hydrolysis yield of 87 %, an enhancement of 77 % compared to the yield from untreated corn stover.
24752927	3	24	dep	showed	396:401	arg1	increased					437:445	increased	437:445	increased	437:445	Chemical composition analysis showed the soluble hemicellulose content increased with pretreatment temperature, whereas the hemicellulose content decreased from 29 to 7 % in pretreated solids.
24752927	2	25	theme	compressed	297:306	arg1	HCW					315:317	HCW	315:317	HCW	315:317	This work investigated physical and chemical changes in plant cell-wall structure of corn stover due to hot compressed water (HCW) pretreatment at 170-190 °C in a tube reactor.
24752927	2	25	theme	compressed	297:306	arg1	water					308:312	hot compressed water	293:312	hot compressed water (HCW) pretreatment at 170-190 °C in a tube reactor	293:363	This work investigated physical and chemical changes in plant cell-wall structure of corn stover due to hot compressed water (HCW) pretreatment at 170-190 °C in a tube reactor.
24752927	3	26	theme	soluble	407:413	arg1	content					429:435	the soluble hemicellulose content	403:435	the soluble hemicellulose content	403:435	Chemical composition analysis showed the soluble hemicellulose content increased with pretreatment temperature, whereas the hemicellulose content decreased from 29 to 7 % in pretreated solids.
24752927	0	27	theme	cell-wall	17:25	arg1	structure					27:35	plant cell-wall structure	11:35	plant cell-wall structure of corn	11:43	Changes in plant cell-wall structure of corn stover due to hot compressed water pretreatment and enhanced enzymatic hydrolysis.
24752927	6	28	theme	enzymatic	981:989	arg1	yield					1002:1006	an enzymatic hydrolysis yield	978:1006	an enzymatic hydrolysis yield of 87 %	978:1014	Enzyme saccharification of solids by optimized pretreatment with HCW at 185 °C resulted in an enzymatic hydrolysis yield of 87 %, an enhancement of 77 % compared to the yield from untreated corn stover.
24752927	2	29	from	°C	344:345	arg1	reactor					357:363	a tube reactor	350:363	a tube reactor	350:363	This work investigated physical and chemical changes in plant cell-wall structure of corn stover due to hot compressed water (HCW) pretreatment at 170-190 °C in a tube reactor.
24752927	2	29	from	°C	344:345	arg1	pretreatment					320:331	hot compressed water (HCW) pretreatment	293:331	hot compressed water (HCW) pretreatment at 170-190 °C in a tube reactor	293:363	This work investigated physical and chemical changes in plant cell-wall structure of corn stover due to hot compressed water (HCW) pretreatment at 170-190 °C in a tube reactor.
24752927	2	30	theme	hot	293:295	arg1	HCW					315:317	HCW	315:317	HCW	315:317	This work investigated physical and chemical changes in plant cell-wall structure of corn stover due to hot compressed water (HCW) pretreatment at 170-190 °C in a tube reactor.
24752927	2	30	theme	hot	293:295	arg1	water					308:312	hot compressed water	293:312	hot compressed water (HCW) pretreatment at 170-190 °C in a tube reactor	293:363	This work investigated physical and chemical changes in plant cell-wall structure of corn stover due to hot compressed water (HCW) pretreatment at 170-190 °C in a tube reactor.
24752927	3	31	theme	pretreatment	452:463	arg1	temperature					465:475	pretreatment temperature	452:475	pretreatment temperature	452:475	Chemical composition analysis showed the soluble hemicellulose content increased with pretreatment temperature, whereas the hemicellulose content decreased from 29 to 7 % in pretreated solids.
24752927	0	32	theme	plant	11:15	arg1	structure					27:35	plant cell-wall structure	11:35	plant cell-wall structure of corn	11:43	Changes in plant cell-wall structure of corn stover due to hot compressed water pretreatment and enhanced enzymatic hydrolysis.
24752927	4	33	theme	Scanning	559:566	arg1	microscopy					577:586	Scanning electron microscopy	559:586	Scanning electron microscopy	559:586	Scanning electron microscopy revealed the parenchyma-type second cell-wall structure of the plant was almost completely removed at 185 °C, and the sclerenchyma-type second cell wall was greatly damaged upon addition of 5 mmol/L ammonium sulfate during HCW pretreatment.
24752927	0	34	theme	enzymatic	106:114	arg1	hydrolysis					116:125	enhanced enzymatic hydrolysis	97:125	enhanced enzymatic hydrolysis	97:125	Changes in plant cell-wall structure of corn stover due to hot compressed water pretreatment and enhanced enzymatic hydrolysis.
24752927	6	35	theme	Enzyme	887:892	arg1	saccharification					894:909	Enzyme saccharification	887:909	Enzyme saccharification of solids by optimized pretreatment with HCW at 185 °C	887:964	Enzyme saccharification of solids by optimized pretreatment with HCW at 185 °C resulted in an enzymatic hydrolysis yield of 87 %, an enhancement of 77 % compared to the yield from untreated corn stover.
24752927	6	36	theme	corn	1077:1080	arg1	stover					1082:1087	untreated corn stover	1067:1087	untreated corn stover	1067:1087	Enzyme saccharification of solids by optimized pretreatment with HCW at 185 °C resulted in an enzymatic hydrolysis yield of 87 %, an enhancement of 77 % compared to the yield from untreated corn stover.
24752927	3	37	dep	7	533:533	arg1	to					530:531	to	530:531	to	530:531	Chemical composition analysis showed the soluble hemicellulose content increased with pretreatment temperature, whereas the hemicellulose content decreased from 29 to 7 % in pretreated solids.
24752927	6	38	theme	%	1038:1038	arg1	enhancement					1020:1030	an enhancement	1017:1030	an enhancement of 77 %	1017:1038	Enzyme saccharification of solids by optimized pretreatment with HCW at 185 °C resulted in an enzymatic hydrolysis yield of 87 %, an enhancement of 77 % compared to the yield from untreated corn stover.
24752927	5	39	theme	enzymatic	869:877	arg1	action					879:884	enzymatic action	869:884	enzymatic action	869:884	These changes favored accessibility for enzymatic action.
24752927	6	40	with	pretreatment	934:945	arg1	HCW					952:954	HCW	952:954	HCW	952:954	Enzyme saccharification of solids by optimized pretreatment with HCW at 185 °C resulted in an enzymatic hydrolysis yield of 87 %, an enhancement of 77 % compared to the yield from untreated corn stover.
24752927	2	41	theme	chemical	225:232	arg1	changes					234:240	physical and chemical changes	212:240	physical and chemical changes in plant cell-wall structure of corn stover due to hot compressed water (HCW) pretreatment at 170-190 °C in a tube reactor	212:363	This work investigated physical and chemical changes in plant cell-wall structure of corn stover due to hot compressed water (HCW) pretreatment at 170-190 °C in a tube reactor.
24752927	4	42	theme	plant	651:655	arg1	structure					634:642	the parenchyma-type second cell-wall structure	597:642	the parenchyma-type second cell-wall structure of the plant	597:655	Scanning electron microscopy revealed the parenchyma-type second cell-wall structure of the plant was almost completely removed at 185 °C, and the sclerenchyma-type second cell wall was greatly damaged upon addition of 5 mmol/L ammonium sulfate during HCW pretreatment.
24752927	3	43	theme	Chemical	366:373	arg1	analysis					387:394	Chemical composition analysis	366:394	Chemical composition analysis	366:394	Chemical composition analysis showed the soluble hemicellulose content increased with pretreatment temperature, whereas the hemicellulose content decreased from 29 to 7 % in pretreated solids.
24752927	4	44	theme	sclerenchyma-type	706:722	arg1	wall					736:739	the sclerenchyma-type second cell wall	702:739	the sclerenchyma-type second cell wall	702:739	Scanning electron microscopy revealed the parenchyma-type second cell-wall structure of the plant was almost completely removed at 185 °C, and the sclerenchyma-type second cell wall was greatly damaged upon addition of 5 mmol/L ammonium sulfate during HCW pretreatment.
24752927	0	45	theme	corn	40:43	arg1	structure					27:35	plant cell-wall structure	11:35	plant cell-wall structure of corn	11:43	Changes in plant cell-wall structure of corn stover due to hot compressed water pretreatment and enhanced enzymatic hydrolysis.
24752927	2	46	theme	physical	212:219	arg1	changes					234:240	physical and chemical changes	212:240	physical and chemical changes in plant cell-wall structure of corn stover due to hot compressed water (HCW) pretreatment at 170-190 °C in a tube reactor	212:363	This work investigated physical and chemical changes in plant cell-wall structure of corn stover due to hot compressed water (HCW) pretreatment at 170-190 °C in a tube reactor.
24752927	3	47	theme	hemicellulose	490:502	arg1	content					504:510	the hemicellulose content	486:510	the hemicellulose content	486:510	Chemical composition analysis showed the soluble hemicellulose content increased with pretreatment temperature, whereas the hemicellulose content decreased from 29 to 7 % in pretreated solids.
24752927	4	48	theme	ammonium	787:794	arg1	sulfate					796:802	5 mmol/L ammonium sulfate	778:802	5 mmol/L ammonium sulfate	778:802	Scanning electron microscopy revealed the parenchyma-type second cell-wall structure of the plant was almost completely removed at 185 °C, and the sclerenchyma-type second cell wall was greatly damaged upon addition of 5 mmol/L ammonium sulfate during HCW pretreatment.
24752927	4	49	theme	HCW	811:813	arg1	pretreatment					815:826	HCW pretreatment	811:826	HCW pretreatment	811:826	Scanning electron microscopy revealed the parenchyma-type second cell-wall structure of the plant was almost completely removed at 185 °C, and the sclerenchyma-type second cell wall was greatly damaged upon addition of 5 mmol/L ammonium sulfate during HCW pretreatment.
24752927	4	50	theme	cell	731:734	arg1	wall					736:739	the sclerenchyma-type second cell wall	702:739	the sclerenchyma-type second cell wall	702:739	Scanning electron microscopy revealed the parenchyma-type second cell-wall structure of the plant was almost completely removed at 185 °C, and the sclerenchyma-type second cell wall was greatly damaged upon addition of 5 mmol/L ammonium sulfate during HCW pretreatment.
24752927	3	51	theme	pretreated	540:549	arg1	solids					551:556	pretreated solids	540:556	pretreated solids	540:556	Chemical composition analysis showed the soluble hemicellulose content increased with pretreatment temperature, whereas the hemicellulose content decreased from 29 to 7 % in pretreated solids.
24752927	4	52	theme	second	617:622	arg1	structure					634:642	the parenchyma-type second cell-wall structure	597:642	the parenchyma-type second cell-wall structure of the plant	597:655	Scanning electron microscopy revealed the parenchyma-type second cell-wall structure of the plant was almost completely removed at 185 °C, and the sclerenchyma-type second cell wall was greatly damaged upon addition of 5 mmol/L ammonium sulfate during HCW pretreatment.
24752927	4	53	theme	5	778:778	arg1	mmol/L					780:785	mmol/L	780:785	mmol/L	780:785	Scanning electron microscopy revealed the parenchyma-type second cell-wall structure of the plant was almost completely removed at 185 °C, and the sclerenchyma-type second cell wall was greatly damaged upon addition of 5 mmol/L ammonium sulfate during HCW pretreatment.
24752927	2	54	theme	cell-wall	251:259	arg1	structure					261:269	plant cell-wall structure	245:269	plant cell-wall structure of corn stover due to hot compressed water (HCW) pretreatment at 170-190 °C in a tube reactor	245:363	This work investigated physical and chemical changes in plant cell-wall structure of corn stover due to hot compressed water (HCW) pretreatment at 170-190 °C in a tube reactor.
24752927	6	55	from	stover	1082:1087	arg1	yield					1056:1060	the yield	1052:1060	the yield from untreated corn stover	1052:1087	Enzyme saccharification of solids by optimized pretreatment with HCW at 185 °C resulted in an enzymatic hydrolysis yield of 87 %, an enhancement of 77 % compared to the yield from untreated corn stover.
24752927	1	56	theme	Corn	128:131	arg1	feedstock					155:163	a potential feedstock	143:163	a potential feedstock for biofuel production	143:186	Corn stover is a potential feedstock for biofuel production.
24752927	1	56	theme	Corn	128:131	arg1	stover					133:138	Corn stover	128:138	Corn stover	128:138	Corn stover is a potential feedstock for biofuel production.
24752927	6	57	dep	resulted	966:973	arg1	compared					1040:1047	compared	1040:1047	compared to the yield from untreated corn stover	1040:1087	Enzyme saccharification of solids by optimized pretreatment with HCW at 185 °C resulted in an enzymatic hydrolysis yield of 87 %, an enhancement of 77 % compared to the yield from untreated corn stover.
24752927	4	58	theme	parenchyma-type	601:615	arg1	structure					634:642	the parenchyma-type second cell-wall structure	597:642	the parenchyma-type second cell-wall structure of the plant	597:655	Scanning electron microscopy revealed the parenchyma-type second cell-wall structure of the plant was almost completely removed at 185 °C, and the sclerenchyma-type second cell wall was greatly damaged upon addition of 5 mmol/L ammonium sulfate during HCW pretreatment.
24752927	2	59	theme	plant	245:249	arg1	structure					261:269	plant cell-wall structure	245:269	plant cell-wall structure of corn stover due to hot compressed water (HCW) pretreatment at 170-190 °C in a tube reactor	245:363	This work investigated physical and chemical changes in plant cell-wall structure of corn stover due to hot compressed water (HCW) pretreatment at 170-190 °C in a tube reactor.
24752927	6	60	theme	optimized	924:932	arg1	pretreatment					934:945	optimized pretreatment	924:945	optimized pretreatment with HCW at 185 °C	924:964	Enzyme saccharification of solids by optimized pretreatment with HCW at 185 °C resulted in an enzymatic hydrolysis yield of 87 %, an enhancement of 77 % compared to the yield from untreated corn stover.
24752927	4	61	theme	sulfate	796:802	arg1	addition					766:773	addition	766:773	addition of 5 mmol/L ammonium sulfate	766:802	Scanning electron microscopy revealed the parenchyma-type second cell-wall structure of the plant was almost completely removed at 185 °C, and the sclerenchyma-type second cell wall was greatly damaged upon addition of 5 mmol/L ammonium sulfate during HCW pretreatment.
24752927	4	62	theme	cell-wall	624:632	arg1	structure					634:642	the parenchyma-type second cell-wall structure	597:642	the parenchyma-type second cell-wall structure of the plant	597:655	Scanning electron microscopy revealed the parenchyma-type second cell-wall structure of the plant was almost completely removed at 185 °C, and the sclerenchyma-type second cell wall was greatly damaged upon addition of 5 mmol/L ammonium sulfate during HCW pretreatment.
28041916	1	0	theme	oxidized	356:363	arg1	OPS					380:382	OPS	380:382	OPS	380:382	Fabrication of novel semi-interpenetrating polymer network (semi-IPN) cryogels by cross-linking polymerization of N,N-dimethylaminoethyl methacrylate (DMAEM) in the presence of either oxidized potato starch (OPS) or oxidized wheat starch (OWS) and their characterization are presented in the paper.
28041916	1	0	theme	oxidized	356:363	arg1	starch					372:377	oxidized potato starch	356:377	oxidized potato starch (OPS)	356:383	Fabrication of novel semi-interpenetrating polymer network (semi-IPN) cryogels by cross-linking polymerization of N,N-dimethylaminoethyl methacrylate (DMAEM) in the presence of either oxidized potato starch (OPS) or oxidized wheat starch (OWS) and their characterization are presented in the paper.
28041916	3	1	theme	sorption-evaporation	854:873	arg1	strategy					875:882	the solvent sorption-evaporation strategy	842:882	the solvent sorption-evaporation strategy	842:882	Indomethacin (IDM), taken as a model anti-inflammatory drug, was easily loaded into the composite cryogels by the solvent sorption-evaporation strategy.
28041916	2	2	from	influence	475:483	arg1	properties					527:536	the properties	523:536	the properties of the composite cryogels	523:562	The influence of the nature of entrapped polymer on the properties of the composite cryogels was evaluated by the swelling kinetics, FT-IR spectroscopy, scanning electron microscopy, and response at external stimuli such as temperature, pH, and ionic strength.
28041916	1	3	theme	potato	365:370	arg1	OPS					380:382	OPS	380:382	OPS	380:382	Fabrication of novel semi-interpenetrating polymer network (semi-IPN) cryogels by cross-linking polymerization of N,N-dimethylaminoethyl methacrylate (DMAEM) in the presence of either oxidized potato starch (OPS) or oxidized wheat starch (OWS) and their characterization are presented in the paper.
28041916	1	3	theme	potato	365:370	arg1	starch					372:377	oxidized potato starch	356:377	oxidized potato starch (OPS)	356:383	Fabrication of novel semi-interpenetrating polymer network (semi-IPN) cryogels by cross-linking polymerization of N,N-dimethylaminoethyl methacrylate (DMAEM) in the presence of either oxidized potato starch (OPS) or oxidized wheat starch (OWS) and their characterization are presented in the paper.
28041916	2	4	theme	swelling	585:592	arg1	kinetics					594:601	the swelling kinetics	581:601	the swelling kinetics	581:601	The influence of the nature of entrapped polymer on the properties of the composite cryogels was evaluated by the swelling kinetics, FT-IR spectroscopy, scanning electron microscopy, and response at external stimuli such as temperature, pH, and ionic strength.
28041916	3	5	theme	composite	820:828	arg1	cryogels					830:837	the composite cryogels	816:837	the composite cryogels	816:837	Indomethacin (IDM), taken as a model anti-inflammatory drug, was easily loaded into the composite cryogels by the solvent sorption-evaporation strategy.
28041916	1	6	theme	N	286:286	arg1	polymerization					268:281	cross-linking polymerization	254:281	cross-linking polymerization of N,N-dimethylaminoethyl methacrylate (DMAEM) in the presence of either oxidized potato starch (OPS) or oxidized wheat starch (OWS)	254:414	Fabrication of novel semi-interpenetrating polymer network (semi-IPN) cryogels by cross-linking polymerization of N,N-dimethylaminoethyl methacrylate (DMAEM) in the presence of either oxidized potato starch (OPS) or oxidized wheat starch (OWS) and their characterization are presented in the paper.
28041916	0	7	theme	cryogels	103:110	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of oxidized starch/poly(N,N-dimethylaminoethyl methacrylate) semi-IPN cryogels and in vitro controlled release evaluation of indomethacin.
28041916	0	7	theme	cryogels	103:110	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of oxidized starch/poly(N,N-dimethylaminoethyl methacrylate) semi-IPN cryogels and in vitro controlled release evaluation of indomethacin.
28041916	1	8	theme	starch	372:377	arg1	presence					337:344	the presence	333:344	the presence of either oxidized potato starch (OPS) or oxidized wheat starch (OWS)	333:414	Fabrication of novel semi-interpenetrating polymer network (semi-IPN) cryogels by cross-linking polymerization of N,N-dimethylaminoethyl methacrylate (DMAEM) in the presence of either oxidized potato starch (OPS) or oxidized wheat starch (OWS) and their characterization are presented in the paper.
28041916	5	9	theme	release	1174:1180	arg1	mechanism					1182:1190	The release mechanism	1170:1190	The release mechanism of IDM from the composite cryogels	1170:1225	The release mechanism of IDM from the composite cryogels was discussed based on two kinetic models, finding that the drug release at 37°C was pseudo-Fickian diffusion, regardless the cryogel composition.
28041916	4	10	theme	polysaccharide	1140:1153	arg1	influence					1113:1121	the influence	1109:1121	the influence of the entrapped polysaccharide	1109:1153	The in vitro release of IDM from the semi-IPN cryogels was low in simulated gastric fluid at pH 1.3, irrespective of the nature of the entrapped oxidized starch, and consistent in simulated intestinal fluid (SIF) at pH 7.4, the influence of the entrapped polysaccharide being evident.
28041916	4	10	theme	polysaccharide	1140:1153	arg1	evident					1161:1167	evident	1161:1167	evident	1161:1167	The in vitro release of IDM from the semi-IPN cryogels was low in simulated gastric fluid at pH 1.3, irrespective of the nature of the entrapped oxidized starch, and consistent in simulated intestinal fluid (SIF) at pH 7.4, the influence of the entrapped polysaccharide being evident.
28041916	0	11	dep	in	116:117	arg1	vitro					119:123	vitro	119:123	vitro	119:123	Preparation and characterization of oxidized starch/poly(N,N-dimethylaminoethyl methacrylate) semi-IPN cryogels and in vitro controlled release evaluation of indomethacin.
28041916	5	12	theme	kinetic	1254:1260	arg1	finding					1270:1276	finding	1270:1276	finding that the drug release at 37°C was pseudo-Fickian diffusion, regardless the cryogel composition	1270:1371	The release mechanism of IDM from the composite cryogels was discussed based on two kinetic models, finding that the drug release at 37°C was pseudo-Fickian diffusion, regardless the cryogel composition.
28041916	5	12	theme	kinetic	1254:1260	arg1	models					1262:1267	two kinetic models	1250:1267	two kinetic models	1250:1267	The release mechanism of IDM from the composite cryogels was discussed based on two kinetic models, finding that the drug release at 37°C was pseudo-Fickian diffusion, regardless the cryogel composition.
28041916	3	13	theme	anti-inflammatory	769:785	arg1	drug					787:790	a model anti-inflammatory drug	761:790	a model anti-inflammatory drug	761:790	Indomethacin (IDM), taken as a model anti-inflammatory drug, was easily loaded into the composite cryogels by the solvent sorption-evaporation strategy.
28041916	0	14	theme	semi-IPN	94:101	arg1	cryogels					103:110	oxidized starch/poly(N,N-dimethylaminoethyl methacrylate) semi-IPN cryogels	36:110	oxidized starch/poly(N,N-dimethylaminoethyl methacrylate) semi-IPN cryogels	36:110	Preparation and characterization of oxidized starch/poly(N,N-dimethylaminoethyl methacrylate) semi-IPN cryogels and in vitro controlled release evaluation of indomethacin.
28041916	4	15	theme	nature	1006:1011	arg1	irrespective					986:997	irrespective	986:997	irrespective	986:997	The in vitro release of IDM from the semi-IPN cryogels was low in simulated gastric fluid at pH 1.3, irrespective of the nature of the entrapped oxidized starch, and consistent in simulated intestinal fluid (SIF) at pH 7.4, the influence of the entrapped polysaccharide being evident.
28041916	2	16	theme	electron	633:640	arg1	microscopy					642:651	scanning electron microscopy	624:651	scanning electron microscopy	624:651	The influence of the nature of entrapped polymer on the properties of the composite cryogels was evaluated by the swelling kinetics, FT-IR spectroscopy, scanning electron microscopy, and response at external stimuli such as temperature, pH, and ionic strength.
28041916	4	17	theme	simulated	1065:1073	arg1	SIF					1093:1095	SIF	1093:1095	SIF	1093:1095	The in vitro release of IDM from the semi-IPN cryogels was low in simulated gastric fluid at pH 1.3, irrespective of the nature of the entrapped oxidized starch, and consistent in simulated intestinal fluid (SIF) at pH 7.4, the influence of the entrapped polysaccharide being evident.
28041916	4	17	theme	simulated	1065:1073	arg1	fluid					1086:1090	simulated intestinal fluid	1065:1090	simulated intestinal fluid (SIF) at pH 7.4	1065:1106	The in vitro release of IDM from the semi-IPN cryogels was low in simulated gastric fluid at pH 1.3, irrespective of the nature of the entrapped oxidized starch, and consistent in simulated intestinal fluid (SIF) at pH 7.4, the influence of the entrapped polysaccharide being evident.
28041916	1	18	theme	N-dimethylaminoethyl	288:307	arg1	DMAEM					323:327	DMAEM	323:327	DMAEM	323:327	Fabrication of novel semi-interpenetrating polymer network (semi-IPN) cryogels by cross-linking polymerization of N,N-dimethylaminoethyl methacrylate (DMAEM) in the presence of either oxidized potato starch (OPS) or oxidized wheat starch (OWS) and their characterization are presented in the paper.
28041916	1	18	theme	N-dimethylaminoethyl	288:307	arg1	N					286:286	N	286:286	N	286:286	Fabrication of novel semi-interpenetrating polymer network (semi-IPN) cryogels by cross-linking polymerization of N,N-dimethylaminoethyl methacrylate (DMAEM) in the presence of either oxidized potato starch (OPS) or oxidized wheat starch (OWS) and their characterization are presented in the paper.
28041916	1	18	theme	N-dimethylaminoethyl	288:307	arg1	methacrylate					309:320	N-dimethylaminoethyl methacrylate	288:320	N-dimethylaminoethyl methacrylate (DMAEM)	288:328	Fabrication of novel semi-interpenetrating polymer network (semi-IPN) cryogels by cross-linking polymerization of N,N-dimethylaminoethyl methacrylate (DMAEM) in the presence of either oxidized potato starch (OPS) or oxidized wheat starch (OWS) and their characterization are presented in the paper.
28041916	0	19	theme	in	116:117	arg1	evaluation					144:153	in vitro controlled release evaluation	116:153	in vitro controlled release evaluation of indomethacin	116:169	Preparation and characterization of oxidized starch/poly(N,N-dimethylaminoethyl methacrylate) semi-IPN cryogels and in vitro controlled release evaluation of indomethacin.
28041916	2	20	theme	scanning	624:631	arg1	microscopy					642:651	scanning electron microscopy	624:651	scanning electron microscopy	624:651	The influence of the nature of entrapped polymer on the properties of the composite cryogels was evaluated by the swelling kinetics, FT-IR spectroscopy, scanning electron microscopy, and response at external stimuli such as temperature, pH, and ionic strength.
28041916	0	21	dep	cryogels	103:110	arg1	methacrylate					80:91	N-dimethylaminoethyl methacrylate	59:91	N-dimethylaminoethyl methacrylate	59:91	Preparation and characterization of oxidized starch/poly(N,N-dimethylaminoethyl methacrylate) semi-IPN cryogels and in vitro controlled release evaluation of indomethacin.
28041916	0	21	dep	cryogels	103:110	arg1	N					57:57	N	57:57	N	57:57	Preparation and characterization of oxidized starch/poly(N,N-dimethylaminoethyl methacrylate) semi-IPN cryogels and in vitro controlled release evaluation of indomethacin.
28041916	4	22	from	fluid	1086:1090	arg1	consistent					1051:1060	consistent	1051:1060	consistent	1051:1060	The in vitro release of IDM from the semi-IPN cryogels was low in simulated gastric fluid at pH 1.3, irrespective of the nature of the entrapped oxidized starch, and consistent in simulated intestinal fluid (SIF) at pH 7.4, the influence of the entrapped polysaccharide being evident.
28041916	4	23	dep	low	944:946	arg1	influence					1113:1121	the influence	1109:1121	the influence of the entrapped polysaccharide	1109:1153	The in vitro release of IDM from the semi-IPN cryogels was low in simulated gastric fluid at pH 1.3, irrespective of the nature of the entrapped oxidized starch, and consistent in simulated intestinal fluid (SIF) at pH 7.4, the influence of the entrapped polysaccharide being evident.
28041916	4	23	dep	low	944:946	arg1	low					944:946	low	944:946	low	944:946	The in vitro release of IDM from the semi-IPN cryogels was low in simulated gastric fluid at pH 1.3, irrespective of the nature of the entrapped oxidized starch, and consistent in simulated intestinal fluid (SIF) at pH 7.4, the influence of the entrapped polysaccharide being evident.
28041916	4	23	dep	low	944:946	arg1	evident					1161:1167	evident	1161:1167	evident	1161:1167	The in vitro release of IDM from the semi-IPN cryogels was low in simulated gastric fluid at pH 1.3, irrespective of the nature of the entrapped oxidized starch, and consistent in simulated intestinal fluid (SIF) at pH 7.4, the influence of the entrapped polysaccharide being evident.
28041916	4	23	dep	low	944:946	arg1	release					898:904	The in vitro release	885:904	The in vitro release of IDM from the semi-IPN cryogels	885:938	The in vitro release of IDM from the semi-IPN cryogels was low in simulated gastric fluid at pH 1.3, irrespective of the nature of the entrapped oxidized starch, and consistent in simulated intestinal fluid (SIF) at pH 7.4, the influence of the entrapped polysaccharide being evident.
28041916	4	24	theme	simulated	951:959	arg1	fluid					969:973	simulated gastric fluid	951:973	simulated gastric fluid at pH 1.3	951:983	The in vitro release of IDM from the semi-IPN cryogels was low in simulated gastric fluid at pH 1.3, irrespective of the nature of the entrapped oxidized starch, and consistent in simulated intestinal fluid (SIF) at pH 7.4, the influence of the entrapped polysaccharide being evident.
28041916	2	25	theme	external	670:677	arg1	pH					708:709	pH	708:709	pH	708:709	The influence of the nature of entrapped polymer on the properties of the composite cryogels was evaluated by the swelling kinetics, FT-IR spectroscopy, scanning electron microscopy, and response at external stimuli such as temperature, pH, and ionic strength.
28041916	2	25	theme	external	670:677	arg1	stimuli					679:685	external stimuli	670:685	external stimuli such as temperature, pH, and ionic strength	670:729	The influence of the nature of entrapped polymer on the properties of the composite cryogels was evaluated by the swelling kinetics, FT-IR spectroscopy, scanning electron microscopy, and response at external stimuli such as temperature, pH, and ionic strength.
28041916	2	25	theme	external	670:677	arg1	temperature					695:705	temperature	695:705	temperature	695:705	The influence of the nature of entrapped polymer on the properties of the composite cryogels was evaluated by the swelling kinetics, FT-IR spectroscopy, scanning electron microscopy, and response at external stimuli such as temperature, pH, and ionic strength.
28041916	2	25	theme	external	670:677	arg1	strength					722:729	ionic strength	716:729	ionic strength	716:729	The influence of the nature of entrapped polymer on the properties of the composite cryogels was evaluated by the swelling kinetics, FT-IR spectroscopy, scanning electron microscopy, and response at external stimuli such as temperature, pH, and ionic strength.
28041916	3	26	theme	solvent	846:852	arg1	strategy					875:882	the solvent sorption-evaporation strategy	842:882	the solvent sorption-evaporation strategy	842:882	Indomethacin (IDM), taken as a model anti-inflammatory drug, was easily loaded into the composite cryogels by the solvent sorption-evaporation strategy.
28041916	1	27	theme	novel	187:191	arg1	cryogels					242:249	novel semi-interpenetrating polymer network (semi-IPN) cryogels	187:249	novel semi-interpenetrating polymer network (semi-IPN) cryogels	187:249	Fabrication of novel semi-interpenetrating polymer network (semi-IPN) cryogels by cross-linking polymerization of N,N-dimethylaminoethyl methacrylate (DMAEM) in the presence of either oxidized potato starch (OPS) or oxidized wheat starch (OWS) and their characterization are presented in the paper.
28041916	4	28	dep	in	889:890	arg1	vitro					892:896	vitro	892:896	vitro	892:896	The in vitro release of IDM from the semi-IPN cryogels was low in simulated gastric fluid at pH 1.3, irrespective of the nature of the entrapped oxidized starch, and consistent in simulated intestinal fluid (SIF) at pH 7.4, the influence of the entrapped polysaccharide being evident.
28041916	2	29	theme	FT-IR	604:608	arg1	spectroscopy					610:621	FT-IR spectroscopy	604:621	FT-IR spectroscopy	604:621	The influence of the nature of entrapped polymer on the properties of the composite cryogels was evaluated by the swelling kinetics, FT-IR spectroscopy, scanning electron microscopy, and response at external stimuli such as temperature, pH, and ionic strength.
28041916	2	30	theme	nature	492:497	arg1	influence					475:483	The influence	471:483	The influence of the nature of entrapped polymer on the properties of the composite cryogels	471:562	The influence of the nature of entrapped polymer on the properties of the composite cryogels was evaluated by the swelling kinetics, FT-IR spectroscopy, scanning electron microscopy, and response at external stimuli such as temperature, pH, and ionic strength.
28041916	2	31	theme	ionic	716:720	arg1	strength					722:729	ionic strength	716:729	ionic strength	716:729	The influence of the nature of entrapped polymer on the properties of the composite cryogels was evaluated by the swelling kinetics, FT-IR spectroscopy, scanning electron microscopy, and response at external stimuli such as temperature, pH, and ionic strength.
28041916	1	32	theme	semi-interpenetrating	193:213	arg1	cryogels					242:249	novel semi-interpenetrating polymer network (semi-IPN) cryogels	187:249	novel semi-interpenetrating polymer network (semi-IPN) cryogels	187:249	Fabrication of novel semi-interpenetrating polymer network (semi-IPN) cryogels by cross-linking polymerization of N,N-dimethylaminoethyl methacrylate (DMAEM) in the presence of either oxidized potato starch (OPS) or oxidized wheat starch (OWS) and their characterization are presented in the paper.
28041916	4	33	theme	entrapped	1130:1138	arg1	polysaccharide					1140:1153	the entrapped polysaccharide	1126:1153	the entrapped polysaccharide	1126:1153	The in vitro release of IDM from the semi-IPN cryogels was low in simulated gastric fluid at pH 1.3, irrespective of the nature of the entrapped oxidized starch, and consistent in simulated intestinal fluid (SIF) at pH 7.4, the influence of the entrapped polysaccharide being evident.
28041916	5	34	theme	cryogel	1353:1359	arg1	diffusion					1327:1335	pseudo-Fickian diffusion	1312:1335	pseudo-Fickian diffusion	1312:1335	The release mechanism of IDM from the composite cryogels was discussed based on two kinetic models, finding that the drug release at 37°C was pseudo-Fickian diffusion, regardless the cryogel composition.
28041916	5	34	theme	cryogel	1353:1359	arg1	composition					1361:1371	the cryogel composition	1349:1371	regardless the cryogel composition	1338:1371	The release mechanism of IDM from the composite cryogels was discussed based on two kinetic models, finding that the drug release at 37°C was pseudo-Fickian diffusion, regardless the cryogel composition.
28041916	1	35	theme	polymer	215:221	arg1	cryogels					242:249	novel semi-interpenetrating polymer network (semi-IPN) cryogels	187:249	novel semi-interpenetrating polymer network (semi-IPN) cryogels	187:249	Fabrication of novel semi-interpenetrating polymer network (semi-IPN) cryogels by cross-linking polymerization of N,N-dimethylaminoethyl methacrylate (DMAEM) in the presence of either oxidized potato starch (OPS) or oxidized wheat starch (OWS) and their characterization are presented in the paper.
28041916	0	36	theme	controlled	125:134	arg1	evaluation					144:153	in vitro controlled release evaluation	116:153	in vitro controlled release evaluation of indomethacin	116:169	Preparation and characterization of oxidized starch/poly(N,N-dimethylaminoethyl methacrylate) semi-IPN cryogels and in vitro controlled release evaluation of indomethacin.
28041916	4	37	theme	starch	1039:1044	arg1	nature					1006:1011	the nature	1002:1011	the nature of the entrapped oxidized starch	1002:1044	The in vitro release of IDM from the semi-IPN cryogels was low in simulated gastric fluid at pH 1.3, irrespective of the nature of the entrapped oxidized starch, and consistent in simulated intestinal fluid (SIF) at pH 7.4, the influence of the entrapped polysaccharide being evident.
28041916	1	38	theme	network	223:229	arg1	cryogels					242:249	novel semi-interpenetrating polymer network (semi-IPN) cryogels	187:249	novel semi-interpenetrating polymer network (semi-IPN) cryogels	187:249	Fabrication of novel semi-interpenetrating polymer network (semi-IPN) cryogels by cross-linking polymerization of N,N-dimethylaminoethyl methacrylate (DMAEM) in the presence of either oxidized potato starch (OPS) or oxidized wheat starch (OWS) and their characterization are presented in the paper.
28041916	4	39	from	pH	978:979	arg1	fluid					969:973	simulated gastric fluid	951:973	simulated gastric fluid at pH 1.3	951:983	The in vitro release of IDM from the semi-IPN cryogels was low in simulated gastric fluid at pH 1.3, irrespective of the nature of the entrapped oxidized starch, and consistent in simulated intestinal fluid (SIF) at pH 7.4, the influence of the entrapped polysaccharide being evident.
28041916	1	40	theme	oxidized	388:395	arg1	starch					403:408	oxidized wheat starch	388:408	oxidized wheat starch (OWS)	388:414	Fabrication of novel semi-interpenetrating polymer network (semi-IPN) cryogels by cross-linking polymerization of N,N-dimethylaminoethyl methacrylate (DMAEM) in the presence of either oxidized potato starch (OPS) or oxidized wheat starch (OWS) and their characterization are presented in the paper.
28041916	1	40	theme	oxidized	388:395	arg1	OWS					411:413	OWS	411:413	OWS	411:413	Fabrication of novel semi-interpenetrating polymer network (semi-IPN) cryogels by cross-linking polymerization of N,N-dimethylaminoethyl methacrylate (DMAEM) in the presence of either oxidized potato starch (OPS) or oxidized wheat starch (OWS) and their characterization are presented in the paper.
28041916	5	41	dep	finding	1270:1276	arg1	diffusion					1327:1335	pseudo-Fickian diffusion	1312:1335	pseudo-Fickian diffusion	1312:1335	The release mechanism of IDM from the composite cryogels was discussed based on two kinetic models, finding that the drug release at 37°C was pseudo-Fickian diffusion, regardless the cryogel composition.
28041916	5	41	dep	finding	1270:1276	arg1	release					1292:1298	the drug release	1283:1298	the drug release at 37°C	1283:1306	The release mechanism of IDM from the composite cryogels was discussed based on two kinetic models, finding that the drug release at 37°C was pseudo-Fickian diffusion, regardless the cryogel composition.
28041916	5	41	dep	finding	1270:1276	arg1	composition					1361:1371	the cryogel composition	1349:1371	regardless the cryogel composition	1338:1371	The release mechanism of IDM from the composite cryogels was discussed based on two kinetic models, finding that the drug release at 37°C was pseudo-Fickian diffusion, regardless the cryogel composition.
28041916	5	42	from	37°C	1303:1306	arg1	diffusion					1327:1335	pseudo-Fickian diffusion	1312:1335	pseudo-Fickian diffusion	1312:1335	The release mechanism of IDM from the composite cryogels was discussed based on two kinetic models, finding that the drug release at 37°C was pseudo-Fickian diffusion, regardless the cryogel composition.
28041916	5	42	from	37°C	1303:1306	arg1	release					1292:1298	the drug release	1283:1298	the drug release at 37°C	1283:1306	The release mechanism of IDM from the composite cryogels was discussed based on two kinetic models, finding that the drug release at 37°C was pseudo-Fickian diffusion, regardless the cryogel composition.
28041916	0	43	theme	starch/poly	45:55	arg1	cryogels					103:110	oxidized starch/poly(N,N-dimethylaminoethyl methacrylate) semi-IPN cryogels	36:110	oxidized starch/poly(N,N-dimethylaminoethyl methacrylate) semi-IPN cryogels	36:110	Preparation and characterization of oxidized starch/poly(N,N-dimethylaminoethyl methacrylate) semi-IPN cryogels and in vitro controlled release evaluation of indomethacin.
28041916	0	44	theme	evaluation	144:153	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of oxidized starch/poly(N,N-dimethylaminoethyl methacrylate) semi-IPN cryogels and in vitro controlled release evaluation of indomethacin.
28041916	0	44	theme	evaluation	144:153	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of oxidized starch/poly(N,N-dimethylaminoethyl methacrylate) semi-IPN cryogels and in vitro controlled release evaluation of indomethacin.
28041916	1	45	theme	wheat	397:401	arg1	starch					403:408	oxidized wheat starch	388:408	oxidized wheat starch (OWS)	388:414	Fabrication of novel semi-interpenetrating polymer network (semi-IPN) cryogels by cross-linking polymerization of N,N-dimethylaminoethyl methacrylate (DMAEM) in the presence of either oxidized potato starch (OPS) or oxidized wheat starch (OWS) and their characterization are presented in the paper.
28041916	1	45	theme	wheat	397:401	arg1	OWS					411:413	OWS	411:413	OWS	411:413	Fabrication of novel semi-interpenetrating polymer network (semi-IPN) cryogels by cross-linking polymerization of N,N-dimethylaminoethyl methacrylate (DMAEM) in the presence of either oxidized potato starch (OPS) or oxidized wheat starch (OWS) and their characterization are presented in the paper.
28041916	5	46	theme	composite	1208:1216	arg1	cryogels					1218:1225	the composite cryogels	1204:1225	the composite cryogels	1204:1225	The release mechanism of IDM from the composite cryogels was discussed based on two kinetic models, finding that the drug release at 37°C was pseudo-Fickian diffusion, regardless the cryogel composition.
28041916	1	47	theme	semi-IPN	232:239	arg1	cryogels					242:249	novel semi-interpenetrating polymer network (semi-IPN) cryogels	187:249	novel semi-interpenetrating polymer network (semi-IPN) cryogels	187:249	Fabrication of novel semi-interpenetrating polymer network (semi-IPN) cryogels by cross-linking polymerization of N,N-dimethylaminoethyl methacrylate (DMAEM) in the presence of either oxidized potato starch (OPS) or oxidized wheat starch (OWS) and their characterization are presented in the paper.
28041916	0	48	theme	oxidized	36:43	arg1	cryogels					103:110	oxidized starch/poly(N,N-dimethylaminoethyl methacrylate) semi-IPN cryogels	36:110	oxidized starch/poly(N,N-dimethylaminoethyl methacrylate) semi-IPN cryogels	36:110	Preparation and characterization of oxidized starch/poly(N,N-dimethylaminoethyl methacrylate) semi-IPN cryogels and in vitro controlled release evaluation of indomethacin.
28041916	4	49	from	fluid	969:973	arg1	release					898:904	The in vitro release	885:904	The in vitro release of IDM from the semi-IPN cryogels	885:938	The in vitro release of IDM from the semi-IPN cryogels was low in simulated gastric fluid at pH 1.3, irrespective of the nature of the entrapped oxidized starch, and consistent in simulated intestinal fluid (SIF) at pH 7.4, the influence of the entrapped polysaccharide being evident.
28041916	4	49	from	fluid	969:973	arg1	evident					1161:1167	evident	1161:1167	evident	1161:1167	The in vitro release of IDM from the semi-IPN cryogels was low in simulated gastric fluid at pH 1.3, irrespective of the nature of the entrapped oxidized starch, and consistent in simulated intestinal fluid (SIF) at pH 7.4, the influence of the entrapped polysaccharide being evident.
28041916	4	49	from	fluid	969:973	arg1	low					944:946	low	944:946	low	944:946	The in vitro release of IDM from the semi-IPN cryogels was low in simulated gastric fluid at pH 1.3, irrespective of the nature of the entrapped oxidized starch, and consistent in simulated intestinal fluid (SIF) at pH 7.4, the influence of the entrapped polysaccharide being evident.
28041916	0	50	theme	release	136:142	arg1	evaluation					144:153	in vitro controlled release evaluation	116:153	in vitro controlled release evaluation of indomethacin	116:169	Preparation and characterization of oxidized starch/poly(N,N-dimethylaminoethyl methacrylate) semi-IPN cryogels and in vitro controlled release evaluation of indomethacin.
28041916	1	51	theme	starch	403:408	arg1	presence					337:344	the presence	333:344	the presence of either oxidized potato starch (OPS) or oxidized wheat starch (OWS)	333:414	Fabrication of novel semi-interpenetrating polymer network (semi-IPN) cryogels by cross-linking polymerization of N,N-dimethylaminoethyl methacrylate (DMAEM) in the presence of either oxidized potato starch (OPS) or oxidized wheat starch (OWS) and their characterization are presented in the paper.
28041916	4	52	from	cryogels	931:938	arg1	low					944:946	low	944:946	low	944:946	The in vitro release of IDM from the semi-IPN cryogels was low in simulated gastric fluid at pH 1.3, irrespective of the nature of the entrapped oxidized starch, and consistent in simulated intestinal fluid (SIF) at pH 7.4, the influence of the entrapped polysaccharide being evident.
28041916	4	52	from	cryogels	931:938	arg1	evident					1161:1167	evident	1161:1167	evident	1161:1167	The in vitro release of IDM from the semi-IPN cryogels was low in simulated gastric fluid at pH 1.3, irrespective of the nature of the entrapped oxidized starch, and consistent in simulated intestinal fluid (SIF) at pH 7.4, the influence of the entrapped polysaccharide being evident.
28041916	4	52	from	cryogels	931:938	arg1	release					898:904	The in vitro release	885:904	The in vitro release of IDM from the semi-IPN cryogels	885:938	The in vitro release of IDM from the semi-IPN cryogels was low in simulated gastric fluid at pH 1.3, irrespective of the nature of the entrapped oxidized starch, and consistent in simulated intestinal fluid (SIF) at pH 7.4, the influence of the entrapped polysaccharide being evident.
28041916	5	53	theme	drug	1287:1290	arg1	diffusion					1327:1335	pseudo-Fickian diffusion	1312:1335	pseudo-Fickian diffusion	1312:1335	The release mechanism of IDM from the composite cryogels was discussed based on two kinetic models, finding that the drug release at 37°C was pseudo-Fickian diffusion, regardless the cryogel composition.
28041916	5	53	theme	drug	1287:1290	arg1	release					1292:1298	the drug release	1283:1298	the drug release at 37°C	1283:1306	The release mechanism of IDM from the composite cryogels was discussed based on two kinetic models, finding that the drug release at 37°C was pseudo-Fickian diffusion, regardless the cryogel composition.
28041916	5	54	theme	pseudo-Fickian	1312:1325	arg1	diffusion					1327:1335	pseudo-Fickian diffusion	1312:1335	pseudo-Fickian diffusion	1312:1335	The release mechanism of IDM from the composite cryogels was discussed based on two kinetic models, finding that the drug release at 37°C was pseudo-Fickian diffusion, regardless the cryogel composition.
28041916	5	54	theme	pseudo-Fickian	1312:1325	arg1	release					1292:1298	the drug release	1283:1298	the drug release at 37°C	1283:1306	The release mechanism of IDM from the composite cryogels was discussed based on two kinetic models, finding that the drug release at 37°C was pseudo-Fickian diffusion, regardless the cryogel composition.
28041916	5	54	theme	pseudo-Fickian	1312:1325	arg1	composition					1361:1371	the cryogel composition	1349:1371	regardless the cryogel composition	1338:1371	The release mechanism of IDM from the composite cryogels was discussed based on two kinetic models, finding that the drug release at 37°C was pseudo-Fickian diffusion, regardless the cryogel composition.
28041916	2	55	theme	entrapped	502:510	arg1	polymer					512:518	entrapped polymer	502:518	entrapped polymer	502:518	The influence of the nature of entrapped polymer on the properties of the composite cryogels was evaluated by the swelling kinetics, FT-IR spectroscopy, scanning electron microscopy, and response at external stimuli such as temperature, pH, and ionic strength.
28041916	4	56	theme	IDM	909:911	arg1	low					944:946	low	944:946	low	944:946	The in vitro release of IDM from the semi-IPN cryogels was low in simulated gastric fluid at pH 1.3, irrespective of the nature of the entrapped oxidized starch, and consistent in simulated intestinal fluid (SIF) at pH 7.4, the influence of the entrapped polysaccharide being evident.
28041916	4	56	theme	IDM	909:911	arg1	evident					1161:1167	evident	1161:1167	evident	1161:1167	The in vitro release of IDM from the semi-IPN cryogels was low in simulated gastric fluid at pH 1.3, irrespective of the nature of the entrapped oxidized starch, and consistent in simulated intestinal fluid (SIF) at pH 7.4, the influence of the entrapped polysaccharide being evident.
28041916	4	56	theme	IDM	909:911	arg1	release					898:904	The in vitro release	885:904	The in vitro release of IDM from the semi-IPN cryogels	885:938	The in vitro release of IDM from the semi-IPN cryogels was low in simulated gastric fluid at pH 1.3, irrespective of the nature of the entrapped oxidized starch, and consistent in simulated intestinal fluid (SIF) at pH 7.4, the influence of the entrapped polysaccharide being evident.
28041916	0	57	theme	indomethacin	158:169	arg1	cryogels					103:110	oxidized starch/poly(N,N-dimethylaminoethyl methacrylate) semi-IPN cryogels	36:110	oxidized starch/poly(N,N-dimethylaminoethyl methacrylate) semi-IPN cryogels	36:110	Preparation and characterization of oxidized starch/poly(N,N-dimethylaminoethyl methacrylate) semi-IPN cryogels and in vitro controlled release evaluation of indomethacin.
28041916	0	57	theme	indomethacin	158:169	arg1	evaluation					144:153	in vitro controlled release evaluation	116:153	in vitro controlled release evaluation of indomethacin	116:169	Preparation and characterization of oxidized starch/poly(N,N-dimethylaminoethyl methacrylate) semi-IPN cryogels and in vitro controlled release evaluation of indomethacin.
28041916	5	58	from	cryogels	1218:1225	arg1	mechanism					1182:1190	The release mechanism	1170:1190	The release mechanism of IDM from the composite cryogels	1170:1225	The release mechanism of IDM from the composite cryogels was discussed based on two kinetic models, finding that the drug release at 37°C was pseudo-Fickian diffusion, regardless the cryogel composition.
28041916	4	59	theme	gastric	961:967	arg1	fluid					969:973	simulated gastric fluid	951:973	simulated gastric fluid at pH 1.3	951:983	The in vitro release of IDM from the semi-IPN cryogels was low in simulated gastric fluid at pH 1.3, irrespective of the nature of the entrapped oxidized starch, and consistent in simulated intestinal fluid (SIF) at pH 7.4, the influence of the entrapped polysaccharide being evident.
28041916	1	60	from	polymerization	268:281	arg1	presence					337:344	the presence	333:344	the presence of either oxidized potato starch (OPS) or oxidized wheat starch (OWS)	333:414	Fabrication of novel semi-interpenetrating polymer network (semi-IPN) cryogels by cross-linking polymerization of N,N-dimethylaminoethyl methacrylate (DMAEM) in the presence of either oxidized potato starch (OPS) or oxidized wheat starch (OWS) and their characterization are presented in the paper.
28041916	4	61	from	pH	1101:1102	arg1	SIF					1093:1095	SIF	1093:1095	SIF	1093:1095	The in vitro release of IDM from the semi-IPN cryogels was low in simulated gastric fluid at pH 1.3, irrespective of the nature of the entrapped oxidized starch, and consistent in simulated intestinal fluid (SIF) at pH 7.4, the influence of the entrapped polysaccharide being evident.
28041916	4	61	from	pH	1101:1102	arg1	fluid					1086:1090	simulated intestinal fluid	1065:1090	simulated intestinal fluid (SIF) at pH 7.4	1065:1106	The in vitro release of IDM from the semi-IPN cryogels was low in simulated gastric fluid at pH 1.3, irrespective of the nature of the entrapped oxidized starch, and consistent in simulated intestinal fluid (SIF) at pH 7.4, the influence of the entrapped polysaccharide being evident.
28041916	5	62	theme	IDM	1195:1197	arg1	mechanism					1182:1190	The release mechanism	1170:1190	The release mechanism of IDM from the composite cryogels	1170:1225	The release mechanism of IDM from the composite cryogels was discussed based on two kinetic models, finding that the drug release at 37°C was pseudo-Fickian diffusion, regardless the cryogel composition.
28041916	2	63	theme	cryogels	555:562	arg1	properties					527:536	the properties	523:536	the properties of the composite cryogels	523:562	The influence of the nature of entrapped polymer on the properties of the composite cryogels was evaluated by the swelling kinetics, FT-IR spectroscopy, scanning electron microscopy, and response at external stimuli such as temperature, pH, and ionic strength.
28041916	1	64	theme	cryogels	242:249	arg1	characterization					426:441	their characterization	420:441	their characterization	420:441	Fabrication of novel semi-interpenetrating polymer network (semi-IPN) cryogels by cross-linking polymerization of N,N-dimethylaminoethyl methacrylate (DMAEM) in the presence of either oxidized potato starch (OPS) or oxidized wheat starch (OWS) and their characterization are presented in the paper.
28041916	1	64	theme	cryogels	242:249	arg1	Fabrication					172:182	Fabrication	172:182	Fabrication of novel semi-interpenetrating polymer network (semi-IPN) cryogels by cross-linking polymerization of N,N-dimethylaminoethyl methacrylate (DMAEM) in the presence of either oxidized potato starch (OPS) or oxidized wheat starch (OWS)	172:414	Fabrication of novel semi-interpenetrating polymer network (semi-IPN) cryogels by cross-linking polymerization of N,N-dimethylaminoethyl methacrylate (DMAEM) in the presence of either oxidized potato starch (OPS) or oxidized wheat starch (OWS) and their characterization are presented in the paper.
28041916	2	65	theme	composite	545:553	arg1	cryogels					555:562	the composite cryogels	541:562	the composite cryogels	541:562	The influence of the nature of entrapped polymer on the properties of the composite cryogels was evaluated by the swelling kinetics, FT-IR spectroscopy, scanning electron microscopy, and response at external stimuli such as temperature, pH, and ionic strength.
28041916	4	66	theme	intestinal	1075:1084	arg1	SIF					1093:1095	SIF	1093:1095	SIF	1093:1095	The in vitro release of IDM from the semi-IPN cryogels was low in simulated gastric fluid at pH 1.3, irrespective of the nature of the entrapped oxidized starch, and consistent in simulated intestinal fluid (SIF) at pH 7.4, the influence of the entrapped polysaccharide being evident.
28041916	4	66	theme	intestinal	1075:1084	arg1	fluid					1086:1090	simulated intestinal fluid	1065:1090	simulated intestinal fluid (SIF) at pH 7.4	1065:1106	The in vitro release of IDM from the semi-IPN cryogels was low in simulated gastric fluid at pH 1.3, irrespective of the nature of the entrapped oxidized starch, and consistent in simulated intestinal fluid (SIF) at pH 7.4, the influence of the entrapped polysaccharide being evident.
28041916	4	67	theme	oxidized	1030:1037	arg1	starch					1039:1044	the entrapped oxidized starch	1016:1044	the entrapped oxidized starch	1016:1044	The in vitro release of IDM from the semi-IPN cryogels was low in simulated gastric fluid at pH 1.3, irrespective of the nature of the entrapped oxidized starch, and consistent in simulated intestinal fluid (SIF) at pH 7.4, the influence of the entrapped polysaccharide being evident.
28041916	3	68	theme	model	763:767	arg1	drug					787:790	a model anti-inflammatory drug	761:790	a model anti-inflammatory drug	761:790	Indomethacin (IDM), taken as a model anti-inflammatory drug, was easily loaded into the composite cryogels by the solvent sorption-evaporation strategy.
28041916	0	69	theme	N-dimethylaminoethyl	59:78	arg1	methacrylate					80:91	N-dimethylaminoethyl methacrylate	59:91	N-dimethylaminoethyl methacrylate	59:91	Preparation and characterization of oxidized starch/poly(N,N-dimethylaminoethyl methacrylate) semi-IPN cryogels and in vitro controlled release evaluation of indomethacin.
28041916	0	69	theme	N-dimethylaminoethyl	59:78	arg1	N					57:57	N	57:57	N	57:57	Preparation and characterization of oxidized starch/poly(N,N-dimethylaminoethyl methacrylate) semi-IPN cryogels and in vitro controlled release evaluation of indomethacin.
28041916	4	70	theme	in	889:890	arg1	low					944:946	low	944:946	low	944:946	The in vitro release of IDM from the semi-IPN cryogels was low in simulated gastric fluid at pH 1.3, irrespective of the nature of the entrapped oxidized starch, and consistent in simulated intestinal fluid (SIF) at pH 7.4, the influence of the entrapped polysaccharide being evident.
28041916	4	70	theme	in	889:890	arg1	evident					1161:1167	evident	1161:1167	evident	1161:1167	The in vitro release of IDM from the semi-IPN cryogels was low in simulated gastric fluid at pH 1.3, irrespective of the nature of the entrapped oxidized starch, and consistent in simulated intestinal fluid (SIF) at pH 7.4, the influence of the entrapped polysaccharide being evident.
28041916	4	70	theme	in	889:890	arg1	release					898:904	The in vitro release	885:904	The in vitro release of IDM from the semi-IPN cryogels	885:938	The in vitro release of IDM from the semi-IPN cryogels was low in simulated gastric fluid at pH 1.3, irrespective of the nature of the entrapped oxidized starch, and consistent in simulated intestinal fluid (SIF) at pH 7.4, the influence of the entrapped polysaccharide being evident.
28041916	4	71	from	consistent	1051:1060	arg1	SIF					1093:1095	SIF	1093:1095	SIF	1093:1095	The in vitro release of IDM from the semi-IPN cryogels was low in simulated gastric fluid at pH 1.3, irrespective of the nature of the entrapped oxidized starch, and consistent in simulated intestinal fluid (SIF) at pH 7.4, the influence of the entrapped polysaccharide being evident.
28041916	4	71	from	consistent	1051:1060	arg1	fluid					1086:1090	simulated intestinal fluid	1065:1090	simulated intestinal fluid (SIF) at pH 7.4	1065:1106	The in vitro release of IDM from the semi-IPN cryogels was low in simulated gastric fluid at pH 1.3, irrespective of the nature of the entrapped oxidized starch, and consistent in simulated intestinal fluid (SIF) at pH 7.4, the influence of the entrapped polysaccharide being evident.
28041916	4	72	theme	entrapped	1020:1028	arg1	starch					1039:1044	the entrapped oxidized starch	1016:1044	the entrapped oxidized starch	1016:1044	The in vitro release of IDM from the semi-IPN cryogels was low in simulated gastric fluid at pH 1.3, irrespective of the nature of the entrapped oxidized starch, and consistent in simulated intestinal fluid (SIF) at pH 7.4, the influence of the entrapped polysaccharide being evident.
28041916	1	73	theme	cross-linking	254:266	arg1	polymerization					268:281	cross-linking polymerization	254:281	cross-linking polymerization of N,N-dimethylaminoethyl methacrylate (DMAEM) in the presence of either oxidized potato starch (OPS) or oxidized wheat starch (OWS)	254:414	Fabrication of novel semi-interpenetrating polymer network (semi-IPN) cryogels by cross-linking polymerization of N,N-dimethylaminoethyl methacrylate (DMAEM) in the presence of either oxidized potato starch (OPS) or oxidized wheat starch (OWS) and their characterization are presented in the paper.
28041916	4	74	theme	semi-IPN	922:929	arg1	cryogels					931:938	the semi-IPN cryogels	918:938	the semi-IPN cryogels	918:938	The in vitro release of IDM from the semi-IPN cryogels was low in simulated gastric fluid at pH 1.3, irrespective of the nature of the entrapped oxidized starch, and consistent in simulated intestinal fluid (SIF) at pH 7.4, the influence of the entrapped polysaccharide being evident.
28041916	4	75	from	low	944:946	arg1	fluid					969:973	simulated gastric fluid	951:973	simulated gastric fluid at pH 1.3	951:983	The in vitro release of IDM from the semi-IPN cryogels was low in simulated gastric fluid at pH 1.3, irrespective of the nature of the entrapped oxidized starch, and consistent in simulated intestinal fluid (SIF) at pH 7.4, the influence of the entrapped polysaccharide being evident.
28041916	2	76	theme	polymer	512:518	arg1	nature					492:497	the nature	488:497	the nature of entrapped polymer	488:518	The influence of the nature of entrapped polymer on the properties of the composite cryogels was evaluated by the swelling kinetics, FT-IR spectroscopy, scanning electron microscopy, and response at external stimuli such as temperature, pH, and ionic strength.
28962782	7	0	theme	nanofibrous	1263:1273	arg1	membrane					1275:1282	the pristine CA nanofibrous membrane	1247:1282	the pristine CA nanofibrous membrane	1247:1282	Char yield of the pristine CA nanofibrous membrane has increased notably from 12 to 24.7% for composite CA10/PolyBA-a5/CTR1 membrane.
28962782	4	1	theme	cross-linked	865:876	arg1	CA10/PolyBA-a5/CTR1					900:918	cross-linked nanofibrous membrane (CA10/PolyBA-a5/CTR1)	865:919	cross-linked nanofibrous membrane (CA10/PolyBA-a5/CTR1)	865:919	SEM images and solubility experiments demonstrated that most favorable result was obtained from the 10% (w/v) CA, 5% (w/v) BA-a and 1% (w/v) CTR composition and cross-linked nanofibrous membrane (CA10/PolyBA-a5/CTR1) was obtained after the thermal curing.
28962782	9	2	theme	i.e.	1748:1751	arg1	compound					1738:1745	a model polycyclic aromatic hydrocarbon (PAH) compound	1692:1745	a model polycyclic aromatic hydrocarbon (PAH) compound (i.e. phenanthrene)	1692:1765	Finally, adsorption performance of pristine CA and CA10/PolyBA-a5/CTR1 nanofibrous membranes was examined by a model polycyclic aromatic hydrocarbon (PAH) compound (i.e. phenanthrene) in aqueous solution, in which CA10/PolyBA-a5/CTR1 nanofibrous membrane has shown better removal efficiency (98.5%) and adsorption capacity (592μg/g).
28962782	9	2	theme	i.e.	1748:1751	arg1	phenanthrene					1753:1764	i.e. phenanthrene	1748:1764	i.e. phenanthrene	1748:1764	Finally, adsorption performance of pristine CA and CA10/PolyBA-a5/CTR1 nanofibrous membranes was examined by a model polycyclic aromatic hydrocarbon (PAH) compound (i.e. phenanthrene) in aqueous solution, in which CA10/PolyBA-a5/CTR1 nanofibrous membrane has shown better removal efficiency (98.5%) and adsorption capacity (592μg/g).
28962782	6	3	theme	nanofibrous	1197:1207	arg1	membranes					1209:1217	CA10/PolyBA-a5/CTR1 nanofibrous membranes	1177:1217	CA10/PolyBA-a5/CTR1 nanofibrous membranes	1177:1217	Thermal, mechanical and adsorption performance of pristine CA and CA10/PolyBA-a5/CTR1 nanofibrous membranes were studied.
28962782	2	4	theme	suitable	360:367	arg1	composition					378:388	suitable solution composition	360:388	suitable solution composition	360:388	Initially, suitable solution composition was determined by varying the amount of the benzoxazine (BA-a) resin, cellulose acetate (CA) and citric acid (CTR) to obtain uniform nanofibrous membrane via electrospinning.
28962782	9	5	theme	CA10/PolyBA-a5/CTR1	1797:1815	arg1	membrane					1829:1836	CA10/PolyBA-a5/CTR1 nanofibrous membrane	1797:1836	CA10/PolyBA-a5/CTR1 nanofibrous membrane	1797:1836	Finally, adsorption performance of pristine CA and CA10/PolyBA-a5/CTR1 nanofibrous membranes was examined by a model polycyclic aromatic hydrocarbon (PAH) compound (i.e. phenanthrene) in aqueous solution, in which CA10/PolyBA-a5/CTR1 nanofibrous membrane has shown better removal efficiency (98.5%) and adsorption capacity (592μg/g).
28962782	1	6	theme	adsorption	261:270	arg1	efficiency					272:281	improved adsorption efficiency	252:281	improved adsorption efficiency	252:281	Herein, polybenzoxazine based cross-linked cellulose acetate nanofibrous membrane exhibiting enhanced thermal/mechanical properties and improved adsorption efficiency was successfully produced via electrospinning and thermal curing.
28962782	9	7	theme	aqueous	1770:1776	arg1	solution					1778:1785	aqueous solution	1770:1785	aqueous solution	1770:1785	Finally, adsorption performance of pristine CA and CA10/PolyBA-a5/CTR1 nanofibrous membranes was examined by a model polycyclic aromatic hydrocarbon (PAH) compound (i.e. phenanthrene) in aqueous solution, in which CA10/PolyBA-a5/CTR1 nanofibrous membrane has shown better removal efficiency (98.5%) and adsorption capacity (592μg/g).
28962782	6	8	theme	pristine	1161:1168	arg1	CA					1170:1171	pristine CA	1161:1171	pristine CA	1161:1171	Thermal, mechanical and adsorption performance of pristine CA and CA10/PolyBA-a5/CTR1 nanofibrous membranes were studied.
28962782	1	9	theme	cellulose	159:167	arg1	membrane					189:196	polybenzoxazine based cross-linked cellulose acetate nanofibrous membrane	124:196	polybenzoxazine based cross-linked cellulose acetate nanofibrous membrane exhibiting enhanced thermal/mechanical properties and improved adsorption efficiency	124:281	Herein, polybenzoxazine based cross-linked cellulose acetate nanofibrous membrane exhibiting enhanced thermal/mechanical properties and improved adsorption efficiency was successfully produced via electrospinning and thermal curing.
28962782	4	10	theme	SEM	704:706	arg1	images					708:713	SEM images	704:713	SEM images	704:713	SEM images and solubility experiments demonstrated that most favorable result was obtained from the 10% (w/v) CA, 5% (w/v) BA-a and 1% (w/v) CTR composition and cross-linked nanofibrous membrane (CA10/PolyBA-a5/CTR1) was obtained after the thermal curing.
28962782	5	11	theme	composite	1079:1087	arg1	membrane					1101:1108	the composite nanofibrous membrane	1075:1108	the composite nanofibrous membrane	1075:1108	Chemical structural changes (ring opening) occurred by thermal curing revealed successful cross-linking of BA-a in the composite nanofibrous membrane.
28962782	9	12	theme	adsorption	1886:1895	arg1	592μg/g					1907:1913	592μg/g	1907:1913	592μg/g	1907:1913	Finally, adsorption performance of pristine CA and CA10/PolyBA-a5/CTR1 nanofibrous membranes was examined by a model polycyclic aromatic hydrocarbon (PAH) compound (i.e. phenanthrene) in aqueous solution, in which CA10/PolyBA-a5/CTR1 nanofibrous membrane has shown better removal efficiency (98.5%) and adsorption capacity (592μg/g).
28962782	9	12	theme	adsorption	1886:1895	arg1	capacity					1897:1904	adsorption capacity	1886:1904	adsorption capacity (592μg/g)	1886:1914	Finally, adsorption performance of pristine CA and CA10/PolyBA-a5/CTR1 nanofibrous membranes was examined by a model polycyclic aromatic hydrocarbon (PAH) compound (i.e. phenanthrene) in aqueous solution, in which CA10/PolyBA-a5/CTR1 nanofibrous membrane has shown better removal efficiency (98.5%) and adsorption capacity (592μg/g).
28962782	4	13	theme	membrane	890:897	arg1	CA10/PolyBA-a5/CTR1					900:918	cross-linked nanofibrous membrane (CA10/PolyBA-a5/CTR1)	865:919	cross-linked nanofibrous membrane (CA10/PolyBA-a5/CTR1)	865:919	SEM images and solubility experiments demonstrated that most favorable result was obtained from the 10% (w/v) CA, 5% (w/v) BA-a and 1% (w/v) CTR composition and cross-linked nanofibrous membrane (CA10/PolyBA-a5/CTR1) was obtained after the thermal curing.
28962782	8	14	theme	Young	1516:1520	arg1	modulus					1524:1530	Young's modulus	1516:1530	Young's modulus	1516:1530	When compared to pristine CA membrane, CA10/PolyBA-a5/CTR1 nanofibrous membrane has shown superior mechanical properties having tensile strength and Young's modulus of 8.64±0.63MPa and 213.87±30.79MPa, respectively.
28962782	5	15	theme	successful	1039:1048	arg1	cross-linking					1050:1062	successful cross-linking	1039:1062	successful cross-linking of BA-a	1039:1070	Chemical structural changes (ring opening) occurred by thermal curing revealed successful cross-linking of BA-a in the composite nanofibrous membrane.
28962782	4	16	dep	%	806:806	arg1	w/v					809:811	w/v	809:811	w/v	809:811	SEM images and solubility experiments demonstrated that most favorable result was obtained from the 10% (w/v) CA, 5% (w/v) BA-a and 1% (w/v) CTR composition and cross-linked nanofibrous membrane (CA10/PolyBA-a5/CTR1) was obtained after the thermal curing.
28962782	9	17	theme	nanofibrous	1817:1827	arg1	membrane					1829:1836	CA10/PolyBA-a5/CTR1 nanofibrous membrane	1797:1836	CA10/PolyBA-a5/CTR1 nanofibrous membrane	1797:1836	Finally, adsorption performance of pristine CA and CA10/PolyBA-a5/CTR1 nanofibrous membranes was examined by a model polycyclic aromatic hydrocarbon (PAH) compound (i.e. phenanthrene) in aqueous solution, in which CA10/PolyBA-a5/CTR1 nanofibrous membrane has shown better removal efficiency (98.5%) and adsorption capacity (592μg/g).
28962782	9	18	theme	membranes	1666:1674	arg1	performance					1603:1613	adsorption performance	1592:1613	adsorption performance of pristine CA and CA10/PolyBA-a5/CTR1 nanofibrous membranes	1592:1674	Finally, adsorption performance of pristine CA and CA10/PolyBA-a5/CTR1 nanofibrous membranes was examined by a model polycyclic aromatic hydrocarbon (PAH) compound (i.e. phenanthrene) in aqueous solution, in which CA10/PolyBA-a5/CTR1 nanofibrous membrane has shown better removal efficiency (98.5%) and adsorption capacity (592μg/g).
28962782	3	19	theme	composite	671:679	arg1	membranes					693:701	cross-linked composite nanofibrous membranes	658:701	cross-linked composite nanofibrous membranes	658:701	Subsequently, thermal curing was performed by step-wise at 150, 175, 200 and 225°C to obtain cross-linked composite nanofibrous membranes.
28962782	1	20	theme	nanofibrous	177:187	arg1	membrane					189:196	polybenzoxazine based cross-linked cellulose acetate nanofibrous membrane	124:196	polybenzoxazine based cross-linked cellulose acetate nanofibrous membrane exhibiting enhanced thermal/mechanical properties and improved adsorption efficiency	124:281	Herein, polybenzoxazine based cross-linked cellulose acetate nanofibrous membrane exhibiting enhanced thermal/mechanical properties and improved adsorption efficiency was successfully produced via electrospinning and thermal curing.
28962782	8	21	theme	8.64±0.63MPa	1535:1546	arg1	strength					1503:1510	tensile strength	1495:1510	tensile strength	1495:1510	When compared to pristine CA membrane, CA10/PolyBA-a5/CTR1 nanofibrous membrane has shown superior mechanical properties having tensile strength and Young's modulus of 8.64±0.63MPa and 213.87±30.79MPa, respectively.
28962782	8	21	theme	8.64±0.63MPa	1535:1546	arg1	modulus					1524:1530	Young's modulus	1516:1530	Young's modulus	1516:1530	When compared to pristine CA membrane, CA10/PolyBA-a5/CTR1 nanofibrous membrane has shown superior mechanical properties having tensile strength and Young's modulus of 8.64±0.63MPa and 213.87±30.79MPa, respectively.
28962782	7	22	theme	CA	1260:1261	arg1	membrane					1275:1282	the pristine CA nanofibrous membrane	1247:1282	the pristine CA nanofibrous membrane	1247:1282	Char yield of the pristine CA nanofibrous membrane has increased notably from 12 to 24.7% for composite CA10/PolyBA-a5/CTR1 membrane.
28962782	4	23	dep	%	837:837	arg1	w/v					840:842	w/v	840:842	w/v	840:842	SEM images and solubility experiments demonstrated that most favorable result was obtained from the 10% (w/v) CA, 5% (w/v) BA-a and 1% (w/v) CTR composition and cross-linked nanofibrous membrane (CA10/PolyBA-a5/CTR1) was obtained after the thermal curing.
28962782	9	24	theme	better	1848:1853	arg1	efficiency					1863:1872	better removal efficiency	1848:1872	better removal efficiency (98.5%)	1848:1880	Finally, adsorption performance of pristine CA and CA10/PolyBA-a5/CTR1 nanofibrous membranes was examined by a model polycyclic aromatic hydrocarbon (PAH) compound (i.e. phenanthrene) in aqueous solution, in which CA10/PolyBA-a5/CTR1 nanofibrous membrane has shown better removal efficiency (98.5%) and adsorption capacity (592μg/g).
28962782	9	24	theme	better	1848:1853	arg1	%					1879:1879	98.5%	1875:1879	98.5%	1875:1879	Finally, adsorption performance of pristine CA and CA10/PolyBA-a5/CTR1 nanofibrous membranes was examined by a model polycyclic aromatic hydrocarbon (PAH) compound (i.e. phenanthrene) in aqueous solution, in which CA10/PolyBA-a5/CTR1 nanofibrous membrane has shown better removal efficiency (98.5%) and adsorption capacity (592μg/g).
28962782	8	25	theme	213.87±30.79MPa	1552:1566	arg1	strength					1503:1510	tensile strength	1495:1510	tensile strength	1495:1510	When compared to pristine CA membrane, CA10/PolyBA-a5/CTR1 nanofibrous membrane has shown superior mechanical properties having tensile strength and Young's modulus of 8.64±0.63MPa and 213.87±30.79MPa, respectively.
28962782	8	25	theme	213.87±30.79MPa	1552:1566	arg1	modulus					1524:1530	Young's modulus	1516:1530	Young's modulus	1516:1530	When compared to pristine CA membrane, CA10/PolyBA-a5/CTR1 nanofibrous membrane has shown superior mechanical properties having tensile strength and Young's modulus of 8.64±0.63MPa and 213.87±30.79MPa, respectively.
28962782	4	26	theme	favorable	765:773	arg1	result					775:780	most favorable result	760:780	most favorable result	760:780	SEM images and solubility experiments demonstrated that most favorable result was obtained from the 10% (w/v) CA, 5% (w/v) BA-a and 1% (w/v) CTR composition and cross-linked nanofibrous membrane (CA10/PolyBA-a5/CTR1) was obtained after the thermal curing.
28962782	1	27	theme	thermal/mechanical	218:235	arg1	properties					237:246	enhanced thermal/mechanical properties	209:246	enhanced thermal/mechanical properties	209:246	Herein, polybenzoxazine based cross-linked cellulose acetate nanofibrous membrane exhibiting enhanced thermal/mechanical properties and improved adsorption efficiency was successfully produced via electrospinning and thermal curing.
28962782	4	28	theme	thermal	944:950	arg1	curing					952:957	the thermal curing	940:957	the thermal curing	940:957	SEM images and solubility experiments demonstrated that most favorable result was obtained from the 10% (w/v) CA, 5% (w/v) BA-a and 1% (w/v) CTR composition and cross-linked nanofibrous membrane (CA10/PolyBA-a5/CTR1) was obtained after the thermal curing.
28962782	4	29	theme	%	806:806	arg1	BA-a					827:830	5% (w/v) BA-a	818:830	5% (w/v) BA-a	818:830	SEM images and solubility experiments demonstrated that most favorable result was obtained from the 10% (w/v) CA, 5% (w/v) BA-a and 1% (w/v) CTR composition and cross-linked nanofibrous membrane (CA10/PolyBA-a5/CTR1) was obtained after the thermal curing.
28962782	4	29	theme	%	806:806	arg1	CA					814:815	the 10% (w/v) CA	800:815	the 10% (w/v) CA	800:815	SEM images and solubility experiments demonstrated that most favorable result was obtained from the 10% (w/v) CA, 5% (w/v) BA-a and 1% (w/v) CTR composition and cross-linked nanofibrous membrane (CA10/PolyBA-a5/CTR1) was obtained after the thermal curing.
28962782	5	30	theme	structural	969:978	arg1	changes					980:986	Chemical structural changes	960:986	Chemical structural changes (ring opening) occurred by thermal curing	960:1028	Chemical structural changes (ring opening) occurred by thermal curing revealed successful cross-linking of BA-a in the composite nanofibrous membrane.
28962782	5	30	theme	structural	969:978	arg1	opening					994:1000	ring opening	989:1000	ring opening	989:1000	Chemical structural changes (ring opening) occurred by thermal curing revealed successful cross-linking of BA-a in the composite nanofibrous membrane.
28962782	8	31	theme	CA	1393:1394	arg1	membrane					1396:1403	pristine CA membrane	1384:1403	pristine CA membrane	1384:1403	When compared to pristine CA membrane, CA10/PolyBA-a5/CTR1 nanofibrous membrane has shown superior mechanical properties having tensile strength and Young's modulus of 8.64±0.63MPa and 213.87±30.79MPa, respectively.
28962782	8	32	contain	having	1488:1493	arg1	properties					1477:1486	superior mechanical properties	1457:1486	superior mechanical properties having tensile strength and Young's modulus of 8.64±0.63MPa and 213.87±30.79MPa, respectively	1457:1580	When compared to pristine CA membrane, CA10/PolyBA-a5/CTR1 nanofibrous membrane has shown superior mechanical properties having tensile strength and Young's modulus of 8.64±0.63MPa and 213.87±30.79MPa, respectively.
28962782	8	32	contain	having	1488:1493	arg2	modulus					1524:1530	Young's modulus	1516:1530	Young's modulus	1516:1530	When compared to pristine CA membrane, CA10/PolyBA-a5/CTR1 nanofibrous membrane has shown superior mechanical properties having tensile strength and Young's modulus of 8.64±0.63MPa and 213.87±30.79MPa, respectively.
28962782	8	32	contain	having	1488:1493	arg2	strength					1503:1510	tensile strength	1495:1510	tensile strength	1495:1510	When compared to pristine CA membrane, CA10/PolyBA-a5/CTR1 nanofibrous membrane has shown superior mechanical properties having tensile strength and Young's modulus of 8.64±0.63MPa and 213.87±30.79MPa, respectively.
28962782	0	33	theme	water	99:103	arg1	treatment					105:113	water treatment	99:113	water treatment	99:113	Fabrication of cellulose acetate/polybenzoxazine cross-linked electrospun nanofibrous membrane for water treatment.
28962782	4	34	theme	10	804:805	arg1	%					806:806	%	806:806	%	806:806	SEM images and solubility experiments demonstrated that most favorable result was obtained from the 10% (w/v) CA, 5% (w/v) BA-a and 1% (w/v) CTR composition and cross-linked nanofibrous membrane (CA10/PolyBA-a5/CTR1) was obtained after the thermal curing.
28962782	2	35	theme	cellulose	460:468	arg1	acetate					470:476	cellulose acetate	460:476	cellulose acetate (CA)	460:481	Initially, suitable solution composition was determined by varying the amount of the benzoxazine (BA-a) resin, cellulose acetate (CA) and citric acid (CTR) to obtain uniform nanofibrous membrane via electrospinning.
28962782	2	35	theme	cellulose	460:468	arg1	CA					479:480	CA	479:480	CA	479:480	Initially, suitable solution composition was determined by varying the amount of the benzoxazine (BA-a) resin, cellulose acetate (CA) and citric acid (CTR) to obtain uniform nanofibrous membrane via electrospinning.
28962782	2	35	theme	cellulose	460:468	arg1	resin					453:457	the benzoxazine (BA-a) resin	430:457	the benzoxazine (BA-a) resin	430:457	Initially, suitable solution composition was determined by varying the amount of the benzoxazine (BA-a) resin, cellulose acetate (CA) and citric acid (CTR) to obtain uniform nanofibrous membrane via electrospinning.
28962782	9	36	theme	nanofibrous	1654:1664	arg1	membranes					1666:1674	CA10/PolyBA-a5/CTR1 nanofibrous membranes	1634:1674	CA10/PolyBA-a5/CTR1 nanofibrous membranes	1634:1674	Finally, adsorption performance of pristine CA and CA10/PolyBA-a5/CTR1 nanofibrous membranes was examined by a model polycyclic aromatic hydrocarbon (PAH) compound (i.e. phenanthrene) in aqueous solution, in which CA10/PolyBA-a5/CTR1 nanofibrous membrane has shown better removal efficiency (98.5%) and adsorption capacity (592μg/g).
28962782	3	37	link	cross-linked	658:669	arg1	membranes					693:701	cross-linked composite nanofibrous membranes	658:701	cross-linked composite nanofibrous membranes	658:701	Subsequently, thermal curing was performed by step-wise at 150, 175, 200 and 225°C to obtain cross-linked composite nanofibrous membranes.
28962782	8	38	theme	nanofibrous	1426:1436	arg1	membrane					1438:1445	CA10/PolyBA-a5/CTR1 nanofibrous membrane	1406:1445	CA10/PolyBA-a5/CTR1 nanofibrous membrane	1406:1445	When compared to pristine CA membrane, CA10/PolyBA-a5/CTR1 nanofibrous membrane has shown superior mechanical properties having tensile strength and Young's modulus of 8.64±0.63MPa and 213.87±30.79MPa, respectively.
28962782	2	39	theme	citric	487:492	arg1	CTR					500:502	CTR	500:502	CTR	500:502	Initially, suitable solution composition was determined by varying the amount of the benzoxazine (BA-a) resin, cellulose acetate (CA) and citric acid (CTR) to obtain uniform nanofibrous membrane via electrospinning.
28962782	2	39	theme	citric	487:492	arg1	acid					494:497	citric acid	487:497	citric acid (CTR)	487:503	Initially, suitable solution composition was determined by varying the amount of the benzoxazine (BA-a) resin, cellulose acetate (CA) and citric acid (CTR) to obtain uniform nanofibrous membrane via electrospinning.
28962782	2	39	theme	citric	487:492	arg1	resin					453:457	the benzoxazine (BA-a) resin	430:457	the benzoxazine (BA-a) resin	430:457	Initially, suitable solution composition was determined by varying the amount of the benzoxazine (BA-a) resin, cellulose acetate (CA) and citric acid (CTR) to obtain uniform nanofibrous membrane via electrospinning.
28962782	0	40	theme	cellulose	15:23	arg1	acetate/polybenzoxazine					25:47	cellulose acetate/polybenzoxazine	15:47	cellulose acetate/polybenzoxazine	15:47	Fabrication of cellulose acetate/polybenzoxazine cross-linked electrospun nanofibrous membrane for water treatment.
28962782	1	41	theme	based	140:144	arg1	membrane					189:196	polybenzoxazine based cross-linked cellulose acetate nanofibrous membrane	124:196	polybenzoxazine based cross-linked cellulose acetate nanofibrous membrane exhibiting enhanced thermal/mechanical properties and improved adsorption efficiency	124:281	Herein, polybenzoxazine based cross-linked cellulose acetate nanofibrous membrane exhibiting enhanced thermal/mechanical properties and improved adsorption efficiency was successfully produced via electrospinning and thermal curing.
28962782	7	42	theme	composite	1327:1335	arg1	membrane					1357:1364	composite CA10/PolyBA-a5/CTR1 membrane	1327:1364	composite CA10/PolyBA-a5/CTR1 membrane	1327:1364	Char yield of the pristine CA nanofibrous membrane has increased notably from 12 to 24.7% for composite CA10/PolyBA-a5/CTR1 membrane.
28962782	2	43	theme	nanofibrous	523:533	arg1	membrane					535:542	uniform nanofibrous membrane	515:542	uniform nanofibrous membrane	515:542	Initially, suitable solution composition was determined by varying the amount of the benzoxazine (BA-a) resin, cellulose acetate (CA) and citric acid (CTR) to obtain uniform nanofibrous membrane via electrospinning.
28962782	5	44	theme	thermal	1015:1021	arg1	curing					1023:1028	thermal curing	1015:1028	thermal curing	1015:1028	Chemical structural changes (ring opening) occurred by thermal curing revealed successful cross-linking of BA-a in the composite nanofibrous membrane.
28962782	8	45	theme	superior	1457:1464	arg1	properties					1477:1486	superior mechanical properties	1457:1486	superior mechanical properties having tensile strength and Young's modulus of 8.64±0.63MPa and 213.87±30.79MPa, respectively	1457:1580	When compared to pristine CA membrane, CA10/PolyBA-a5/CTR1 nanofibrous membrane has shown superior mechanical properties having tensile strength and Young's modulus of 8.64±0.63MPa and 213.87±30.79MPa, respectively.
28962782	2	46	theme	benzoxazine	434:444	arg1	acetate					470:476	cellulose acetate	460:476	cellulose acetate (CA)	460:481	Initially, suitable solution composition was determined by varying the amount of the benzoxazine (BA-a) resin, cellulose acetate (CA) and citric acid (CTR) to obtain uniform nanofibrous membrane via electrospinning.
28962782	2	46	theme	benzoxazine	434:444	arg1	acid					494:497	citric acid	487:497	citric acid (CTR)	487:503	Initially, suitable solution composition was determined by varying the amount of the benzoxazine (BA-a) resin, cellulose acetate (CA) and citric acid (CTR) to obtain uniform nanofibrous membrane via electrospinning.
28962782	2	46	theme	benzoxazine	434:444	arg1	resin					453:457	the benzoxazine (BA-a) resin	430:457	the benzoxazine (BA-a) resin	430:457	Initially, suitable solution composition was determined by varying the amount of the benzoxazine (BA-a) resin, cellulose acetate (CA) and citric acid (CTR) to obtain uniform nanofibrous membrane via electrospinning.
28962782	0	47	theme	nanofibrous	74:84	arg1	membrane					86:93	electrospun nanofibrous membrane	62:93	electrospun nanofibrous membrane	62:93	Fabrication of cellulose acetate/polybenzoxazine cross-linked electrospun nanofibrous membrane for water treatment.
28962782	9	48	theme	model	1694:1698	arg1	PAH					1733:1735	PAH	1733:1735	PAH	1733:1735	Finally, adsorption performance of pristine CA and CA10/PolyBA-a5/CTR1 nanofibrous membranes was examined by a model polycyclic aromatic hydrocarbon (PAH) compound (i.e. phenanthrene) in aqueous solution, in which CA10/PolyBA-a5/CTR1 nanofibrous membrane has shown better removal efficiency (98.5%) and adsorption capacity (592μg/g).
28962782	9	48	theme	model	1694:1698	arg1	hydrocarbon					1720:1730	a model polycyclic aromatic hydrocarbon	1692:1730	a model polycyclic aromatic hydrocarbon (PAH) compound (i.e. phenanthrene)	1692:1765	Finally, adsorption performance of pristine CA and CA10/PolyBA-a5/CTR1 nanofibrous membranes was examined by a model polycyclic aromatic hydrocarbon (PAH) compound (i.e. phenanthrene) in aqueous solution, in which CA10/PolyBA-a5/CTR1 nanofibrous membrane has shown better removal efficiency (98.5%) and adsorption capacity (592μg/g).
28962782	6	49	theme	adsorption	1135:1144	arg1	performance					1146:1156	Thermal, mechanical and adsorption performance	1111:1156	Thermal, mechanical and adsorption performance of pristine CA and CA10/PolyBA-a5/CTR1 nanofibrous membranes	1111:1217	Thermal, mechanical and adsorption performance of pristine CA and CA10/PolyBA-a5/CTR1 nanofibrous membranes were studied.
28962782	1	50	link	cross-linked	146:157	arg1	membrane					189:196	polybenzoxazine based cross-linked cellulose acetate nanofibrous membrane	124:196	polybenzoxazine based cross-linked cellulose acetate nanofibrous membrane exhibiting enhanced thermal/mechanical properties and improved adsorption efficiency	124:281	Herein, polybenzoxazine based cross-linked cellulose acetate nanofibrous membrane exhibiting enhanced thermal/mechanical properties and improved adsorption efficiency was successfully produced via electrospinning and thermal curing.
28962782	4	51	theme	%	819:819	arg1	BA-a					827:830	5% (w/v) BA-a	818:830	5% (w/v) BA-a	818:830	SEM images and solubility experiments demonstrated that most favorable result was obtained from the 10% (w/v) CA, 5% (w/v) BA-a and 1% (w/v) CTR composition and cross-linked nanofibrous membrane (CA10/PolyBA-a5/CTR1) was obtained after the thermal curing.
28962782	4	51	theme	%	819:819	arg1	CA					814:815	the 10% (w/v) CA	800:815	the 10% (w/v) CA	800:815	SEM images and solubility experiments demonstrated that most favorable result was obtained from the 10% (w/v) CA, 5% (w/v) BA-a and 1% (w/v) CTR composition and cross-linked nanofibrous membrane (CA10/PolyBA-a5/CTR1) was obtained after the thermal curing.
28962782	9	52	theme	aromatic	1711:1718	arg1	PAH					1733:1735	PAH	1733:1735	PAH	1733:1735	Finally, adsorption performance of pristine CA and CA10/PolyBA-a5/CTR1 nanofibrous membranes was examined by a model polycyclic aromatic hydrocarbon (PAH) compound (i.e. phenanthrene) in aqueous solution, in which CA10/PolyBA-a5/CTR1 nanofibrous membrane has shown better removal efficiency (98.5%) and adsorption capacity (592μg/g).
28962782	9	52	theme	aromatic	1711:1718	arg1	hydrocarbon					1720:1730	a model polycyclic aromatic hydrocarbon	1692:1730	a model polycyclic aromatic hydrocarbon (PAH) compound (i.e. phenanthrene)	1692:1765	Finally, adsorption performance of pristine CA and CA10/PolyBA-a5/CTR1 nanofibrous membranes was examined by a model polycyclic aromatic hydrocarbon (PAH) compound (i.e. phenanthrene) in aqueous solution, in which CA10/PolyBA-a5/CTR1 nanofibrous membrane has shown better removal efficiency (98.5%) and adsorption capacity (592μg/g).
28962782	6	53	theme	mechanical	1120:1129	arg1	performance					1146:1156	Thermal, mechanical and adsorption performance	1111:1156	Thermal, mechanical and adsorption performance of pristine CA and CA10/PolyBA-a5/CTR1 nanofibrous membranes	1111:1217	Thermal, mechanical and adsorption performance of pristine CA and CA10/PolyBA-a5/CTR1 nanofibrous membranes were studied.
28962782	8	54	theme	tensile	1495:1501	arg1	strength					1503:1510	tensile strength	1495:1510	tensile strength	1495:1510	When compared to pristine CA membrane, CA10/PolyBA-a5/CTR1 nanofibrous membrane has shown superior mechanical properties having tensile strength and Young's modulus of 8.64±0.63MPa and 213.87±30.79MPa, respectively.
28962782	3	55	theme	thermal	579:585	arg1	curing					587:592	thermal curing	579:592	thermal curing	579:592	Subsequently, thermal curing was performed by step-wise at 150, 175, 200 and 225°C to obtain cross-linked composite nanofibrous membranes.
28962782	6	56	theme	Thermal	1111:1117	arg1	performance					1146:1156	Thermal, mechanical and adsorption performance	1111:1156	Thermal, mechanical and adsorption performance of pristine CA and CA10/PolyBA-a5/CTR1 nanofibrous membranes	1111:1217	Thermal, mechanical and adsorption performance of pristine CA and CA10/PolyBA-a5/CTR1 nanofibrous membranes were studied.
28962782	2	57	theme	BA-a	447:450	arg1	acetate					470:476	cellulose acetate	460:476	cellulose acetate (CA)	460:481	Initially, suitable solution composition was determined by varying the amount of the benzoxazine (BA-a) resin, cellulose acetate (CA) and citric acid (CTR) to obtain uniform nanofibrous membrane via electrospinning.
28962782	2	57	theme	BA-a	447:450	arg1	acid					494:497	citric acid	487:497	citric acid (CTR)	487:503	Initially, suitable solution composition was determined by varying the amount of the benzoxazine (BA-a) resin, cellulose acetate (CA) and citric acid (CTR) to obtain uniform nanofibrous membrane via electrospinning.
28962782	2	57	theme	BA-a	447:450	arg1	resin					453:457	the benzoxazine (BA-a) resin	430:457	the benzoxazine (BA-a) resin	430:457	Initially, suitable solution composition was determined by varying the amount of the benzoxazine (BA-a) resin, cellulose acetate (CA) and citric acid (CTR) to obtain uniform nanofibrous membrane via electrospinning.
28962782	4	58	theme	CTR	845:847	arg1	composition					849:859	1% (w/v) CTR composition	836:859	1% (w/v) CTR composition	836:859	SEM images and solubility experiments demonstrated that most favorable result was obtained from the 10% (w/v) CA, 5% (w/v) BA-a and 1% (w/v) CTR composition and cross-linked nanofibrous membrane (CA10/PolyBA-a5/CTR1) was obtained after the thermal curing.
28962782	7	59	theme	membrane	1275:1282	arg1	yield					1238:1242	Char yield	1233:1242	Char yield of the pristine CA nanofibrous membrane	1233:1282	Char yield of the pristine CA nanofibrous membrane has increased notably from 12 to 24.7% for composite CA10/PolyBA-a5/CTR1 membrane.
28962782	4	60	theme	1	836:836	arg1	%					837:837	%	837:837	%	837:837	SEM images and solubility experiments demonstrated that most favorable result was obtained from the 10% (w/v) CA, 5% (w/v) BA-a and 1% (w/v) CTR composition and cross-linked nanofibrous membrane (CA10/PolyBA-a5/CTR1) was obtained after the thermal curing.
28962782	6	61	theme	membranes	1209:1217	arg1	performance					1146:1156	Thermal, mechanical and adsorption performance	1111:1156	Thermal, mechanical and adsorption performance of pristine CA and CA10/PolyBA-a5/CTR1 nanofibrous membranes	1111:1217	Thermal, mechanical and adsorption performance of pristine CA and CA10/PolyBA-a5/CTR1 nanofibrous membranes were studied.
28962782	2	62	theme	solution	369:376	arg1	composition					378:388	suitable solution composition	360:388	suitable solution composition	360:388	Initially, suitable solution composition was determined by varying the amount of the benzoxazine (BA-a) resin, cellulose acetate (CA) and citric acid (CTR) to obtain uniform nanofibrous membrane via electrospinning.
28962782	4	63	dep	%	819:819	arg1	w/v					822:824	w/v	822:824	w/v	822:824	SEM images and solubility experiments demonstrated that most favorable result was obtained from the 10% (w/v) CA, 5% (w/v) BA-a and 1% (w/v) CTR composition and cross-linked nanofibrous membrane (CA10/PolyBA-a5/CTR1) was obtained after the thermal curing.
28962782	4	64	theme	%	837:837	arg1	composition					849:859	1% (w/v) CTR composition	836:859	1% (w/v) CTR composition	836:859	SEM images and solubility experiments demonstrated that most favorable result was obtained from the 10% (w/v) CA, 5% (w/v) BA-a and 1% (w/v) CTR composition and cross-linked nanofibrous membrane (CA10/PolyBA-a5/CTR1) was obtained after the thermal curing.
28962782	6	65	theme	CA10/PolyBA-a5/CTR1	1177:1195	arg1	membranes					1209:1217	CA10/PolyBA-a5/CTR1 nanofibrous membranes	1177:1217	CA10/PolyBA-a5/CTR1 nanofibrous membranes	1177:1217	Thermal, mechanical and adsorption performance of pristine CA and CA10/PolyBA-a5/CTR1 nanofibrous membranes were studied.
28962782	4	66	theme	images	708:713	arg1	experiments					730:740	SEM images and solubility experiments	704:740	SEM images and solubility experiments	704:740	SEM images and solubility experiments demonstrated that most favorable result was obtained from the 10% (w/v) CA, 5% (w/v) BA-a and 1% (w/v) CTR composition and cross-linked nanofibrous membrane (CA10/PolyBA-a5/CTR1) was obtained after the thermal curing.
28962782	4	67	link	cross-linked	865:876	arg1	CA10/PolyBA-a5/CTR1					900:918	cross-linked nanofibrous membrane (CA10/PolyBA-a5/CTR1)	865:919	cross-linked nanofibrous membrane (CA10/PolyBA-a5/CTR1)	865:919	SEM images and solubility experiments demonstrated that most favorable result was obtained from the 10% (w/v) CA, 5% (w/v) BA-a and 1% (w/v) CTR composition and cross-linked nanofibrous membrane (CA10/PolyBA-a5/CTR1) was obtained after the thermal curing.
28962782	1	68	theme	improved	252:259	arg1	efficiency					272:281	improved adsorption efficiency	252:281	improved adsorption efficiency	252:281	Herein, polybenzoxazine based cross-linked cellulose acetate nanofibrous membrane exhibiting enhanced thermal/mechanical properties and improved adsorption efficiency was successfully produced via electrospinning and thermal curing.
28962782	6	69	theme	CA	1170:1171	arg1	performance					1146:1156	Thermal, mechanical and adsorption performance	1111:1156	Thermal, mechanical and adsorption performance of pristine CA and CA10/PolyBA-a5/CTR1 nanofibrous membranes	1111:1217	Thermal, mechanical and adsorption performance of pristine CA and CA10/PolyBA-a5/CTR1 nanofibrous membranes were studied.
28962782	4	70	theme	solubility	719:728	arg1	experiments					730:740	SEM images and solubility experiments	704:740	SEM images and solubility experiments	704:740	SEM images and solubility experiments demonstrated that most favorable result was obtained from the 10% (w/v) CA, 5% (w/v) BA-a and 1% (w/v) CTR composition and cross-linked nanofibrous membrane (CA10/PolyBA-a5/CTR1) was obtained after the thermal curing.
28962782	9	71	theme	polycyclic	1700:1709	arg1	PAH					1733:1735	PAH	1733:1735	PAH	1733:1735	Finally, adsorption performance of pristine CA and CA10/PolyBA-a5/CTR1 nanofibrous membranes was examined by a model polycyclic aromatic hydrocarbon (PAH) compound (i.e. phenanthrene) in aqueous solution, in which CA10/PolyBA-a5/CTR1 nanofibrous membrane has shown better removal efficiency (98.5%) and adsorption capacity (592μg/g).
28962782	9	71	theme	polycyclic	1700:1709	arg1	hydrocarbon					1720:1730	a model polycyclic aromatic hydrocarbon	1692:1730	a model polycyclic aromatic hydrocarbon (PAH) compound (i.e. phenanthrene)	1692:1765	Finally, adsorption performance of pristine CA and CA10/PolyBA-a5/CTR1 nanofibrous membranes was examined by a model polycyclic aromatic hydrocarbon (PAH) compound (i.e. phenanthrene) in aqueous solution, in which CA10/PolyBA-a5/CTR1 nanofibrous membrane has shown better removal efficiency (98.5%) and adsorption capacity (592μg/g).
28962782	9	72	theme	adsorption	1592:1601	arg1	performance					1603:1613	adsorption performance	1592:1613	adsorption performance of pristine CA and CA10/PolyBA-a5/CTR1 nanofibrous membranes	1592:1674	Finally, adsorption performance of pristine CA and CA10/PolyBA-a5/CTR1 nanofibrous membranes was examined by a model polycyclic aromatic hydrocarbon (PAH) compound (i.e. phenanthrene) in aqueous solution, in which CA10/PolyBA-a5/CTR1 nanofibrous membrane has shown better removal efficiency (98.5%) and adsorption capacity (592μg/g).
28962782	5	73	theme	nanofibrous	1089:1099	arg1	membrane					1101:1108	the composite nanofibrous membrane	1075:1108	the composite nanofibrous membrane	1075:1108	Chemical structural changes (ring opening) occurred by thermal curing revealed successful cross-linking of BA-a in the composite nanofibrous membrane.
28962782	1	74	theme	cross-linked	146:157	arg1	membrane					189:196	polybenzoxazine based cross-linked cellulose acetate nanofibrous membrane	124:196	polybenzoxazine based cross-linked cellulose acetate nanofibrous membrane exhibiting enhanced thermal/mechanical properties and improved adsorption efficiency	124:281	Herein, polybenzoxazine based cross-linked cellulose acetate nanofibrous membrane exhibiting enhanced thermal/mechanical properties and improved adsorption efficiency was successfully produced via electrospinning and thermal curing.
28962782	4	75	theme	nanofibrous	878:888	arg1	CA10/PolyBA-a5/CTR1					900:918	cross-linked nanofibrous membrane (CA10/PolyBA-a5/CTR1)	865:919	cross-linked nanofibrous membrane (CA10/PolyBA-a5/CTR1)	865:919	SEM images and solubility experiments demonstrated that most favorable result was obtained from the 10% (w/v) CA, 5% (w/v) BA-a and 1% (w/v) CTR composition and cross-linked nanofibrous membrane (CA10/PolyBA-a5/CTR1) was obtained after the thermal curing.
28962782	5	76	theme	ring	989:992	arg1	changes					980:986	Chemical structural changes	960:986	Chemical structural changes (ring opening) occurred by thermal curing	960:1028	Chemical structural changes (ring opening) occurred by thermal curing revealed successful cross-linking of BA-a in the composite nanofibrous membrane.
28962782	5	76	theme	ring	989:992	arg1	opening					994:1000	ring opening	989:1000	ring opening	989:1000	Chemical structural changes (ring opening) occurred by thermal curing revealed successful cross-linking of BA-a in the composite nanofibrous membrane.
28962782	3	77	theme	cross-linked	658:669	arg1	membranes					693:701	cross-linked composite nanofibrous membranes	658:701	cross-linked composite nanofibrous membranes	658:701	Subsequently, thermal curing was performed by step-wise at 150, 175, 200 and 225°C to obtain cross-linked composite nanofibrous membranes.
28962782	1	78	theme	acetate	169:175	arg1	membrane					189:196	polybenzoxazine based cross-linked cellulose acetate nanofibrous membrane	124:196	polybenzoxazine based cross-linked cellulose acetate nanofibrous membrane exhibiting enhanced thermal/mechanical properties and improved adsorption efficiency	124:281	Herein, polybenzoxazine based cross-linked cellulose acetate nanofibrous membrane exhibiting enhanced thermal/mechanical properties and improved adsorption efficiency was successfully produced via electrospinning and thermal curing.
28962782	7	79	theme	pristine	1251:1258	arg1	membrane					1275:1282	the pristine CA nanofibrous membrane	1247:1282	the pristine CA nanofibrous membrane	1247:1282	Char yield of the pristine CA nanofibrous membrane has increased notably from 12 to 24.7% for composite CA10/PolyBA-a5/CTR1 membrane.
28962782	5	80	theme	BA-a	1067:1070	arg1	cross-linking					1050:1062	successful cross-linking	1039:1062	successful cross-linking of BA-a	1039:1070	Chemical structural changes (ring opening) occurred by thermal curing revealed successful cross-linking of BA-a in the composite nanofibrous membrane.
28962782	1	81	theme	enhanced	209:216	arg1	properties					237:246	enhanced thermal/mechanical properties	209:246	enhanced thermal/mechanical properties	209:246	Herein, polybenzoxazine based cross-linked cellulose acetate nanofibrous membrane exhibiting enhanced thermal/mechanical properties and improved adsorption efficiency was successfully produced via electrospinning and thermal curing.
28962782	9	82	theme	removal	1855:1861	arg1	efficiency					1863:1872	better removal efficiency	1848:1872	better removal efficiency (98.5%)	1848:1880	Finally, adsorption performance of pristine CA and CA10/PolyBA-a5/CTR1 nanofibrous membranes was examined by a model polycyclic aromatic hydrocarbon (PAH) compound (i.e. phenanthrene) in aqueous solution, in which CA10/PolyBA-a5/CTR1 nanofibrous membrane has shown better removal efficiency (98.5%) and adsorption capacity (592μg/g).
28962782	9	82	theme	removal	1855:1861	arg1	%					1879:1879	98.5%	1875:1879	98.5%	1875:1879	Finally, adsorption performance of pristine CA and CA10/PolyBA-a5/CTR1 nanofibrous membranes was examined by a model polycyclic aromatic hydrocarbon (PAH) compound (i.e. phenanthrene) in aqueous solution, in which CA10/PolyBA-a5/CTR1 nanofibrous membrane has shown better removal efficiency (98.5%) and adsorption capacity (592μg/g).
28962782	7	83	theme	Char	1233:1236	arg1	yield					1238:1242	Char yield	1233:1242	Char yield of the pristine CA nanofibrous membrane	1233:1282	Char yield of the pristine CA nanofibrous membrane has increased notably from 12 to 24.7% for composite CA10/PolyBA-a5/CTR1 membrane.
28962782	8	84	theme	mechanical	1466:1475	arg1	properties					1477:1486	superior mechanical properties	1457:1486	superior mechanical properties having tensile strength and Young's modulus of 8.64±0.63MPa and 213.87±30.79MPa, respectively	1457:1580	When compared to pristine CA membrane, CA10/PolyBA-a5/CTR1 nanofibrous membrane has shown superior mechanical properties having tensile strength and Young's modulus of 8.64±0.63MPa and 213.87±30.79MPa, respectively.
28962782	5	85	theme	Chemical	960:967	arg1	changes					980:986	Chemical structural changes	960:986	Chemical structural changes (ring opening) occurred by thermal curing	960:1028	Chemical structural changes (ring opening) occurred by thermal curing revealed successful cross-linking of BA-a in the composite nanofibrous membrane.
28962782	5	85	theme	Chemical	960:967	arg1	opening					994:1000	ring opening	989:1000	ring opening	989:1000	Chemical structural changes (ring opening) occurred by thermal curing revealed successful cross-linking of BA-a in the composite nanofibrous membrane.
28962782	9	86	theme	pristine	1618:1625	arg1	CA					1627:1628	pristine CA	1618:1628	pristine CA	1618:1628	Finally, adsorption performance of pristine CA and CA10/PolyBA-a5/CTR1 nanofibrous membranes was examined by a model polycyclic aromatic hydrocarbon (PAH) compound (i.e. phenanthrene) in aqueous solution, in which CA10/PolyBA-a5/CTR1 nanofibrous membrane has shown better removal efficiency (98.5%) and adsorption capacity (592μg/g).
28962782	9	87	theme	CA	1627:1628	arg1	performance					1603:1613	adsorption performance	1592:1613	adsorption performance of pristine CA and CA10/PolyBA-a5/CTR1 nanofibrous membranes	1592:1674	Finally, adsorption performance of pristine CA and CA10/PolyBA-a5/CTR1 nanofibrous membranes was examined by a model polycyclic aromatic hydrocarbon (PAH) compound (i.e. phenanthrene) in aqueous solution, in which CA10/PolyBA-a5/CTR1 nanofibrous membrane has shown better removal efficiency (98.5%) and adsorption capacity (592μg/g).
28962782	8	88	theme	pristine	1384:1391	arg1	membrane					1396:1403	pristine CA membrane	1384:1403	pristine CA membrane	1384:1403	When compared to pristine CA membrane, CA10/PolyBA-a5/CTR1 nanofibrous membrane has shown superior mechanical properties having tensile strength and Young's modulus of 8.64±0.63MPa and 213.87±30.79MPa, respectively.
28962782	9	89	theme	CA10/PolyBA-a5/CTR1	1634:1652	arg1	membranes					1666:1674	CA10/PolyBA-a5/CTR1 nanofibrous membranes	1634:1674	CA10/PolyBA-a5/CTR1 nanofibrous membranes	1634:1674	Finally, adsorption performance of pristine CA and CA10/PolyBA-a5/CTR1 nanofibrous membranes was examined by a model polycyclic aromatic hydrocarbon (PAH) compound (i.e. phenanthrene) in aqueous solution, in which CA10/PolyBA-a5/CTR1 nanofibrous membrane has shown better removal efficiency (98.5%) and adsorption capacity (592μg/g).
28962782	7	90	theme	CA10/PolyBA-a5/CTR1	1337:1355	arg1	membrane					1357:1364	composite CA10/PolyBA-a5/CTR1 membrane	1327:1364	composite CA10/PolyBA-a5/CTR1 membrane	1327:1364	Char yield of the pristine CA nanofibrous membrane has increased notably from 12 to 24.7% for composite CA10/PolyBA-a5/CTR1 membrane.
28962782	0	91	theme	acetate/polybenzoxazine	25:47	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of cellulose acetate/polybenzoxazine	0:47	Fabrication of cellulose acetate/polybenzoxazine cross-linked electrospun nanofibrous membrane for water treatment.
28962782	1	92	theme	polybenzoxazine	124:138	arg1	membrane					189:196	polybenzoxazine based cross-linked cellulose acetate nanofibrous membrane	124:196	polybenzoxazine based cross-linked cellulose acetate nanofibrous membrane exhibiting enhanced thermal/mechanical properties and improved adsorption efficiency	124:281	Herein, polybenzoxazine based cross-linked cellulose acetate nanofibrous membrane exhibiting enhanced thermal/mechanical properties and improved adsorption efficiency was successfully produced via electrospinning and thermal curing.
28962782	8	93	theme	CA10/PolyBA-a5/CTR1	1406:1424	arg1	membrane					1438:1445	CA10/PolyBA-a5/CTR1 nanofibrous membrane	1406:1445	CA10/PolyBA-a5/CTR1 nanofibrous membrane	1406:1445	When compared to pristine CA membrane, CA10/PolyBA-a5/CTR1 nanofibrous membrane has shown superior mechanical properties having tensile strength and Young's modulus of 8.64±0.63MPa and 213.87±30.79MPa, respectively.
28962782	2	94	theme	resin	453:457	arg1	acetate					470:476	cellulose acetate	460:476	cellulose acetate (CA)	460:481	Initially, suitable solution composition was determined by varying the amount of the benzoxazine (BA-a) resin, cellulose acetate (CA) and citric acid (CTR) to obtain uniform nanofibrous membrane via electrospinning.
28962782	2	94	theme	resin	453:457	arg1	amount					420:425	the amount	416:425	the amount of the benzoxazine (BA-a) resin, cellulose acetate (CA) and citric acid (CTR) to obtain uniform nanofibrous membrane via electrospinning	416:562	Initially, suitable solution composition was determined by varying the amount of the benzoxazine (BA-a) resin, cellulose acetate (CA) and citric acid (CTR) to obtain uniform nanofibrous membrane via electrospinning.
28962782	2	94	theme	resin	453:457	arg1	acid					494:497	citric acid	487:497	citric acid (CTR)	487:503	Initially, suitable solution composition was determined by varying the amount of the benzoxazine (BA-a) resin, cellulose acetate (CA) and citric acid (CTR) to obtain uniform nanofibrous membrane via electrospinning.
28962782	2	94	theme	resin	453:457	arg1	resin					453:457	the benzoxazine (BA-a) resin	430:457	the benzoxazine (BA-a) resin	430:457	Initially, suitable solution composition was determined by varying the amount of the benzoxazine (BA-a) resin, cellulose acetate (CA) and citric acid (CTR) to obtain uniform nanofibrous membrane via electrospinning.
28962782	0	95	theme	electrospun	62:72	arg1	membrane					86:93	electrospun nanofibrous membrane	62:93	electrospun nanofibrous membrane	62:93	Fabrication of cellulose acetate/polybenzoxazine cross-linked electrospun nanofibrous membrane for water treatment.
28962782	2	96	theme	uniform	515:521	arg1	membrane					535:542	uniform nanofibrous membrane	515:542	uniform nanofibrous membrane	515:542	Initially, suitable solution composition was determined by varying the amount of the benzoxazine (BA-a) resin, cellulose acetate (CA) and citric acid (CTR) to obtain uniform nanofibrous membrane via electrospinning.
28962782	3	97	theme	nanofibrous	681:691	arg1	membranes					693:701	cross-linked composite nanofibrous membranes	658:701	cross-linked composite nanofibrous membranes	658:701	Subsequently, thermal curing was performed by step-wise at 150, 175, 200 and 225°C to obtain cross-linked composite nanofibrous membranes.
28962782	7	98	dep	24.7	1317:1320	arg1	to					1314:1315	to	1314:1315	to	1314:1315	Char yield of the pristine CA nanofibrous membrane has increased notably from 12 to 24.7% for composite CA10/PolyBA-a5/CTR1 membrane.
28962782	4	99	theme	5	818:818	arg1	%					819:819	%	819:819	%	819:819	SEM images and solubility experiments demonstrated that most favorable result was obtained from the 10% (w/v) CA, 5% (w/v) BA-a and 1% (w/v) CTR composition and cross-linked nanofibrous membrane (CA10/PolyBA-a5/CTR1) was obtained after the thermal curing.
28962782	9	100	theme	hydrocarbon	1720:1730	arg1	compound					1738:1745	a model polycyclic aromatic hydrocarbon (PAH) compound	1692:1745	a model polycyclic aromatic hydrocarbon (PAH) compound (i.e. phenanthrene)	1692:1765	Finally, adsorption performance of pristine CA and CA10/PolyBA-a5/CTR1 nanofibrous membranes was examined by a model polycyclic aromatic hydrocarbon (PAH) compound (i.e. phenanthrene) in aqueous solution, in which CA10/PolyBA-a5/CTR1 nanofibrous membrane has shown better removal efficiency (98.5%) and adsorption capacity (592μg/g).
28962782	9	100	theme	hydrocarbon	1720:1730	arg1	phenanthrene					1753:1764	i.e. phenanthrene	1748:1764	i.e. phenanthrene	1748:1764	Finally, adsorption performance of pristine CA and CA10/PolyBA-a5/CTR1 nanofibrous membranes was examined by a model polycyclic aromatic hydrocarbon (PAH) compound (i.e. phenanthrene) in aqueous solution, in which CA10/PolyBA-a5/CTR1 nanofibrous membrane has shown better removal efficiency (98.5%) and adsorption capacity (592μg/g).
28962782	1	101	theme	thermal	333:339	arg1	curing					341:346	thermal curing	333:346	thermal curing	333:346	Herein, polybenzoxazine based cross-linked cellulose acetate nanofibrous membrane exhibiting enhanced thermal/mechanical properties and improved adsorption efficiency was successfully produced via electrospinning and thermal curing.
28258007	8	0	theme	phosphate	1226:1234	arg1	removal					1236:1242	phosphate removal	1226:1242	phosphate removal	1226:1242	The mechanism of phosphate removal by M@CSBent composites was governed by ion-exchange, electrostatic attraction and inner sphere complexation.
28258007	5	1	theme	batch	1033:1037	arg1	mode					1039:1042	batch mode	1033:1042	batch mode	1033:1042	The systematic study for various adsorption influenced parameters such as agitation time, presence of co-existent anions, solution pH, temperature and initial phosphate concentration has been verified in batch mode.
28258007	1	2	theme	eutrophication	144:157	arg1	problem					133:139	the problem	129:139	the problem of eutrophication due to phosphate	129:174	In this present work, to overcome the problem of eutrophication due to phosphate an attempt was made to develop eco-friendly composite materials using chitosan and bentonite.
28258007	5	3	theme	solution	951:958	arg1	pH					960:961	solution pH	951:961	solution pH	951:961	The systematic study for various adsorption influenced parameters such as agitation time, presence of co-existent anions, solution pH, temperature and initial phosphate concentration has been verified in batch mode.
28258007	4	4	theme	CSBent	811:816	arg1	composite					818:826	the individual CSBent composite	796:826	the individual CSBent composite	796:826	The synthesized Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites possess the efficient phosphate sorption capacities (SCs) of 40.86, 22.15 and 13.44mg/g than the individual CSBent composite.
28258007	3	5	theme	CSBent	603:608	arg1	biocomposites					610:622	Ca@CSBent biocomposites	600:622	Ca@CSBent biocomposites	600:622	To enhance the uptake capacity of CSBent, various multivalent metal ions like Zr4+, Fe3+ and Ca2+ were imprinted on CSBent composites namely Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites respectively.
28258007	4	6	theme	40.86	764:768	arg1	SCs					756:758	SCs	756:758	SCs	756:758	The synthesized Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites possess the efficient phosphate sorption capacities (SCs) of 40.86, 22.15 and 13.44mg/g than the individual CSBent composite.
28258007	4	6	theme	40.86	764:768	arg1	capacities					744:753	the efficient phosphate sorption capacities	711:753	the efficient phosphate sorption capacities (SCs) of 40.86, 22.15 and 13.44mg/g than the individual CSBent composite	711:826	The synthesized Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites possess the efficient phosphate sorption capacities (SCs) of 40.86, 22.15 and 13.44mg/g than the individual CSBent composite.
28258007	8	7	theme	sphere	1332:1337	arg1	complexation					1339:1350	inner sphere complexation	1326:1350	inner sphere complexation	1326:1350	The mechanism of phosphate removal by M@CSBent composites was governed by ion-exchange, electrostatic attraction and inner sphere complexation.
28258007	4	8	theme	22.15	771:775	arg1	SCs					756:758	SCs	756:758	SCs	756:758	The synthesized Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites possess the efficient phosphate sorption capacities (SCs) of 40.86, 22.15 and 13.44mg/g than the individual CSBent composite.
28258007	4	8	theme	22.15	771:775	arg1	capacities					744:753	the efficient phosphate sorption capacities	711:753	the efficient phosphate sorption capacities (SCs) of 40.86, 22.15 and 13.44mg/g than the individual CSBent composite	711:826	The synthesized Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites possess the efficient phosphate sorption capacities (SCs) of 40.86, 22.15 and 13.44mg/g than the individual CSBent composite.
28258007	4	9	theme	CSBent	682:687	arg1	biocomposites					689:701	The synthesized Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites	638:701	The synthesized Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites	638:701	The synthesized Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites possess the efficient phosphate sorption capacities (SCs) of 40.86, 22.15 and 13.44mg/g than the individual CSBent composite.
28258007	5	10	theme	co-existent	931:941	arg1	anions					943:948	co-existent anions	931:948	co-existent anions	931:948	The systematic study for various adsorption influenced parameters such as agitation time, presence of co-existent anions, solution pH, temperature and initial phosphate concentration has been verified in batch mode.
28258007	5	11	theme	adsorption	862:871	arg1	time					913:916	agitation time	903:916	agitation time	903:916	The systematic study for various adsorption influenced parameters such as agitation time, presence of co-existent anions, solution pH, temperature and initial phosphate concentration has been verified in batch mode.
28258007	5	11	theme	adsorption	862:871	arg1	pH					960:961	solution pH	951:961	solution pH	951:961	The systematic study for various adsorption influenced parameters such as agitation time, presence of co-existent anions, solution pH, temperature and initial phosphate concentration has been verified in batch mode.
28258007	5	11	theme	adsorption	862:871	arg1	concentration					998:1010	initial phosphate concentration	980:1010	initial phosphate concentration	980:1010	The systematic study for various adsorption influenced parameters such as agitation time, presence of co-existent anions, solution pH, temperature and initial phosphate concentration has been verified in batch mode.
28258007	5	11	theme	adsorption	862:871	arg1	parameters					884:893	various adsorption influenced parameters	854:893	various adsorption influenced parameters such as agitation time, presence of co-existent anions, solution pH, temperature and initial phosphate concentration	854:1010	The systematic study for various adsorption influenced parameters such as agitation time, presence of co-existent anions, solution pH, temperature and initial phosphate concentration has been verified in batch mode.
28258007	5	11	theme	adsorption	862:871	arg1	presence					919:926	presence	919:926	presence of co-existent anions	919:948	The systematic study for various adsorption influenced parameters such as agitation time, presence of co-existent anions, solution pH, temperature and initial phosphate concentration has been verified in batch mode.
28258007	5	11	theme	adsorption	862:871	arg1	temperature					964:974	temperature	964:974	temperature	964:974	The systematic study for various adsorption influenced parameters such as agitation time, presence of co-existent anions, solution pH, temperature and initial phosphate concentration has been verified in batch mode.
28258007	5	12	theme	agitation	903:911	arg1	time					913:916	agitation time	903:916	agitation time	903:916	The systematic study for various adsorption influenced parameters such as agitation time, presence of co-existent anions, solution pH, temperature and initial phosphate concentration has been verified in batch mode.
28258007	4	13	theme	CSBent	668:673	arg1	biocomposites					689:701	The synthesized Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites	638:701	The synthesized Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites	638:701	The synthesized Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites possess the efficient phosphate sorption capacities (SCs) of 40.86, 22.15 and 13.44mg/g than the individual CSBent composite.
28258007	3	14	theme	CSBent	468:473	arg1	capacity					456:463	the uptake capacity	445:463	the uptake capacity of CSBent	445:473	To enhance the uptake capacity of CSBent, various multivalent metal ions like Zr4+, Fe3+ and Ca2+ were imprinted on CSBent composites namely Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites respectively.
28258007	4	15	theme	Zr	654:655	arg1	CSBent					657:662	Zr@CSBent	654:662	Zr@CSBent	654:662	The synthesized Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites possess the efficient phosphate sorption capacities (SCs) of 40.86, 22.15 and 13.44mg/g than the individual CSBent composite.
28258007	2	16	used	utilized	402:409	arg2	composites					373:382	chitosan supported bentonite (CSBent) composites	335:382	chitosan supported bentonite (CSBent) composites	335:382	To improve the properties of bentonite (bent) and chitosan (CS), chitosan supported bentonite (CSBent) composites were prepared and utilized for phosphate removal.
28258007	3	17	theme	metal	496:500	arg1	ions					502:505	various multivalent metal ions	476:505	various multivalent metal ions like Zr4+, Fe3+ and Ca2+	476:530	To enhance the uptake capacity of CSBent, various multivalent metal ions like Zr4+, Fe3+ and Ca2+ were imprinted on CSBent composites namely Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites respectively.
28258007	2	18	theme	CSBent	365:370	arg1	composites					373:382	chitosan supported bentonite (CSBent) composites	335:382	chitosan supported bentonite (CSBent) composites	335:382	To improve the properties of bentonite (bent) and chitosan (CS), chitosan supported bentonite (CSBent) composites were prepared and utilized for phosphate removal.
28258007	6	19	theme	prepared	1049:1056	arg1	biocomposites					1058:1070	The prepared biocomposites	1045:1070	The prepared biocomposites	1045:1070	The prepared biocomposites was exemplified by FTIR, TEM, SEM and EDAX analysis.
28258007	4	20	theme	Ca	679:680	arg1	CSBent					682:687	Ca@CSBent	679:687	Ca@CSBent	679:687	The synthesized Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites possess the efficient phosphate sorption capacities (SCs) of 40.86, 22.15 and 13.44mg/g than the individual CSBent composite.
28258007	2	21	theme	phosphate	415:423	arg1	removal					425:431	phosphate removal	415:431	phosphate removal	415:431	To improve the properties of bentonite (bent) and chitosan (CS), chitosan supported bentonite (CSBent) composites were prepared and utilized for phosphate removal.
28258007	5	22	theme	systematic	833:842	arg1	study					844:848	The systematic study	829:848	The systematic study for various adsorption influenced parameters such as agitation time, presence of co-existent anions, solution pH, temperature and initial phosphate concentration	829:1010	The systematic study for various adsorption influenced parameters such as agitation time, presence of co-existent anions, solution pH, temperature and initial phosphate concentration has been verified in batch mode.
28258007	7	23	theme	various	1161:1167	arg1	isotherms					1169:1177	various isotherms	1161:1177	various isotherms	1161:1177	The experimental data was fitted to various isotherms and thermodynamic parameters.
28258007	2	24	theme	bentonite	354:362	arg1	composites					373:382	chitosan supported bentonite (CSBent) composites	335:382	chitosan supported bentonite (CSBent) composites	335:382	To improve the properties of bentonite (bent) and chitosan (CS), chitosan supported bentonite (CSBent) composites were prepared and utilized for phosphate removal.
28258007	4	25	contain	possess	703:709	arg2	capacities					744:753	the efficient phosphate sorption capacities	711:753	the efficient phosphate sorption capacities (SCs) of 40.86, 22.15 and 13.44mg/g than the individual CSBent composite	711:826	The synthesized Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites possess the efficient phosphate sorption capacities (SCs) of 40.86, 22.15 and 13.44mg/g than the individual CSBent composite.
28258007	4	25	contain	possess	703:709	arg1	biocomposites					689:701	The synthesized Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites	638:701	The synthesized Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites	638:701	The synthesized Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites possess the efficient phosphate sorption capacities (SCs) of 40.86, 22.15 and 13.44mg/g than the individual CSBent composite.
28258007	4	25	contain	possess	703:709	arg2	SCs					756:758	SCs	756:758	SCs	756:758	The synthesized Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites possess the efficient phosphate sorption capacities (SCs) of 40.86, 22.15 and 13.44mg/g than the individual CSBent composite.
28258007	8	26	theme	CSBent	1249:1254	arg1	composites					1256:1265	M@CSBent composites	1247:1265	M@CSBent composites	1247:1265	The mechanism of phosphate removal by M@CSBent composites was governed by ion-exchange, electrostatic attraction and inner sphere complexation.
28258007	2	27	theme	supported	344:352	arg1	composites					373:382	chitosan supported bentonite (CSBent) composites	335:382	chitosan supported bentonite (CSBent) composites	335:382	To improve the properties of bentonite (bent) and chitosan (CS), chitosan supported bentonite (CSBent) composites were prepared and utilized for phosphate removal.
28258007	0	28	theme	metal	27:31	arg1	ions					33:36	multivalent metal ions	15:36	multivalent metal ions	15:36	Development of multivalent metal ions imprinted chitosan biocomposites for phosphate sorption.
28258007	4	29	theme	CSBent	657:662	arg1	biocomposites					689:701	The synthesized Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites	638:701	The synthesized Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites	638:701	The synthesized Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites possess the efficient phosphate sorption capacities (SCs) of 40.86, 22.15 and 13.44mg/g than the individual CSBent composite.
28258007	5	30	theme	anions	943:948	arg1	time					913:916	agitation time	903:916	agitation time	903:916	The systematic study for various adsorption influenced parameters such as agitation time, presence of co-existent anions, solution pH, temperature and initial phosphate concentration has been verified in batch mode.
28258007	5	30	theme	anions	943:948	arg1	pH					960:961	solution pH	951:961	solution pH	951:961	The systematic study for various adsorption influenced parameters such as agitation time, presence of co-existent anions, solution pH, temperature and initial phosphate concentration has been verified in batch mode.
28258007	5	30	theme	anions	943:948	arg1	concentration					998:1010	initial phosphate concentration	980:1010	initial phosphate concentration	980:1010	The systematic study for various adsorption influenced parameters such as agitation time, presence of co-existent anions, solution pH, temperature and initial phosphate concentration has been verified in batch mode.
28258007	5	30	theme	anions	943:948	arg1	temperature					964:974	temperature	964:974	temperature	964:974	The systematic study for various adsorption influenced parameters such as agitation time, presence of co-existent anions, solution pH, temperature and initial phosphate concentration has been verified in batch mode.
28258007	5	30	theme	anions	943:948	arg1	presence					919:926	presence	919:926	presence of co-existent anions	919:948	The systematic study for various adsorption influenced parameters such as agitation time, presence of co-existent anions, solution pH, temperature and initial phosphate concentration has been verified in batch mode.
28258007	2	31	theme	bentonite	299:307	arg1	properties					285:294	the properties	281:294	the properties of bentonite (bent) and chitosan (CS)	281:332	To improve the properties of bentonite (bent) and chitosan (CS), chitosan supported bentonite (CSBent) composites were prepared and utilized for phosphate removal.
28258007	5	32	theme	various	854:860	arg1	time					913:916	agitation time	903:916	agitation time	903:916	The systematic study for various adsorption influenced parameters such as agitation time, presence of co-existent anions, solution pH, temperature and initial phosphate concentration has been verified in batch mode.
28258007	5	32	theme	various	854:860	arg1	pH					960:961	solution pH	951:961	solution pH	951:961	The systematic study for various adsorption influenced parameters such as agitation time, presence of co-existent anions, solution pH, temperature and initial phosphate concentration has been verified in batch mode.
28258007	5	32	theme	various	854:860	arg1	concentration					998:1010	initial phosphate concentration	980:1010	initial phosphate concentration	980:1010	The systematic study for various adsorption influenced parameters such as agitation time, presence of co-existent anions, solution pH, temperature and initial phosphate concentration has been verified in batch mode.
28258007	5	32	theme	various	854:860	arg1	parameters					884:893	various adsorption influenced parameters	854:893	various adsorption influenced parameters such as agitation time, presence of co-existent anions, solution pH, temperature and initial phosphate concentration	854:1010	The systematic study for various adsorption influenced parameters such as agitation time, presence of co-existent anions, solution pH, temperature and initial phosphate concentration has been verified in batch mode.
28258007	5	32	theme	various	854:860	arg1	presence					919:926	presence	919:926	presence of co-existent anions	919:948	The systematic study for various adsorption influenced parameters such as agitation time, presence of co-existent anions, solution pH, temperature and initial phosphate concentration has been verified in batch mode.
28258007	5	32	theme	various	854:860	arg1	temperature					964:974	temperature	964:974	temperature	964:974	The systematic study for various adsorption influenced parameters such as agitation time, presence of co-existent anions, solution pH, temperature and initial phosphate concentration has been verified in batch mode.
28258007	3	33	dep	composites	557:566	arg1	CSBent					578:583	Zr@CSBent	575:583	Zr@CSBent	575:583	To enhance the uptake capacity of CSBent, various multivalent metal ions like Zr4+, Fe3+ and Ca2+ were imprinted on CSBent composites namely Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites respectively.
28258007	3	33	dep	composites	557:566	arg1	composites					557:566	CSBent composites	550:566	CSBent composites namely Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites respectively	550:635	To enhance the uptake capacity of CSBent, various multivalent metal ions like Zr4+, Fe3+ and Ca2+ were imprinted on CSBent composites namely Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites respectively.
28258007	3	33	dep	composites	557:566	arg1	biocomposites					610:622	Ca@CSBent biocomposites	600:622	Ca@CSBent biocomposites	600:622	To enhance the uptake capacity of CSBent, various multivalent metal ions like Zr4+, Fe3+ and Ca2+ were imprinted on CSBent composites namely Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites respectively.
28258007	3	33	dep	composites	557:566	arg1	CSBent					589:594	Fe@CSBent	586:594	Fe@CSBent	586:594	To enhance the uptake capacity of CSBent, various multivalent metal ions like Zr4+, Fe3+ and Ca2+ were imprinted on CSBent composites namely Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites respectively.
28258007	0	34	theme	multivalent	15:25	arg1	ions					33:36	multivalent metal ions	15:36	multivalent metal ions	15:36	Development of multivalent metal ions imprinted chitosan biocomposites for phosphate sorption.
28258007	4	35	theme	@	681:681	arg1	CSBent					682:687	Ca@CSBent	679:687	Ca@CSBent	679:687	The synthesized Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites possess the efficient phosphate sorption capacities (SCs) of 40.86, 22.15 and 13.44mg/g than the individual CSBent composite.
28258007	3	36	theme	Fe	586:587	arg1	composites					557:566	CSBent composites	550:566	CSBent composites namely Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites respectively	550:635	To enhance the uptake capacity of CSBent, various multivalent metal ions like Zr4+, Fe3+ and Ca2+ were imprinted on CSBent composites namely Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites respectively.
28258007	3	36	theme	Fe	586:587	arg1	CSBent					589:594	Fe@CSBent	586:594	Fe@CSBent	586:594	To enhance the uptake capacity of CSBent, various multivalent metal ions like Zr4+, Fe3+ and Ca2+ were imprinted on CSBent composites namely Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites respectively.
28258007	4	37	theme	Fe	665:666	arg1	CSBent					668:673	Fe@CSBent	665:673	Fe@CSBent	665:673	The synthesized Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites possess the efficient phosphate sorption capacities (SCs) of 40.86, 22.15 and 13.44mg/g than the individual CSBent composite.
28258007	3	38	theme	various	476:482	arg1	ions					502:505	various multivalent metal ions	476:505	various multivalent metal ions like Zr4+, Fe3+ and Ca2+	476:530	To enhance the uptake capacity of CSBent, various multivalent metal ions like Zr4+, Fe3+ and Ca2+ were imprinted on CSBent composites namely Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites respectively.
28258007	8	39	theme	electrostatic	1297:1309	arg1	attraction					1311:1320	electrostatic attraction	1297:1320	electrostatic attraction	1297:1320	The mechanism of phosphate removal by M@CSBent composites was governed by ion-exchange, electrostatic attraction and inner sphere complexation.
28258007	9	40	theme	phosphate	1407:1415	arg1	uptake					1417:1422	phosphate uptake	1407:1422	phosphate uptake	1407:1422	This study reveals a feasibility of biocomposites for phosphate uptake from polluted water sample at field situation.
28258007	3	41	theme	@	588:588	arg1	composites					557:566	CSBent composites	550:566	CSBent composites namely Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites respectively	550:635	To enhance the uptake capacity of CSBent, various multivalent metal ions like Zr4+, Fe3+ and Ca2+ were imprinted on CSBent composites namely Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites respectively.
28258007	3	41	theme	@	588:588	arg1	CSBent					589:594	Fe@CSBent	586:594	Fe@CSBent	586:594	To enhance the uptake capacity of CSBent, various multivalent metal ions like Zr4+, Fe3+ and Ca2+ were imprinted on CSBent composites namely Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites respectively.
28258007	4	42	theme	phosphate	725:733	arg1	SCs					756:758	SCs	756:758	SCs	756:758	The synthesized Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites possess the efficient phosphate sorption capacities (SCs) of 40.86, 22.15 and 13.44mg/g than the individual CSBent composite.
28258007	4	42	theme	phosphate	725:733	arg1	capacities					744:753	the efficient phosphate sorption capacities	711:753	the efficient phosphate sorption capacities (SCs) of 40.86, 22.15 and 13.44mg/g than the individual CSBent composite	711:826	The synthesized Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites possess the efficient phosphate sorption capacities (SCs) of 40.86, 22.15 and 13.44mg/g than the individual CSBent composite.
28258007	7	43	theme	thermodynamic	1183:1195	arg1	parameters					1197:1206	thermodynamic parameters	1183:1206	thermodynamic parameters	1183:1206	The experimental data was fitted to various isotherms and thermodynamic parameters.
28258007	0	44	theme	ions	33:36	arg1	Development					0:10	Development	0:10	Development of multivalent metal ions	0:36	Development of multivalent metal ions imprinted chitosan biocomposites for phosphate sorption.
28258007	5	45	theme	initial	980:986	arg1	concentration					998:1010	initial phosphate concentration	980:1010	initial phosphate concentration	980:1010	The systematic study for various adsorption influenced parameters such as agitation time, presence of co-existent anions, solution pH, temperature and initial phosphate concentration has been verified in batch mode.
28258007	3	46	theme	CSBent	550:555	arg1	CSBent					578:583	Zr@CSBent	575:583	Zr@CSBent	575:583	To enhance the uptake capacity of CSBent, various multivalent metal ions like Zr4+, Fe3+ and Ca2+ were imprinted on CSBent composites namely Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites respectively.
28258007	3	46	theme	CSBent	550:555	arg1	CSBent					589:594	Fe@CSBent	586:594	Fe@CSBent	586:594	To enhance the uptake capacity of CSBent, various multivalent metal ions like Zr4+, Fe3+ and Ca2+ were imprinted on CSBent composites namely Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites respectively.
28258007	3	46	theme	CSBent	550:555	arg1	composites					557:566	CSBent composites	550:566	CSBent composites namely Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites respectively	550:635	To enhance the uptake capacity of CSBent, various multivalent metal ions like Zr4+, Fe3+ and Ca2+ were imprinted on CSBent composites namely Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites respectively.
28258007	4	47	theme	@	667:667	arg1	CSBent					668:673	Fe@CSBent	665:673	Fe@CSBent	665:673	The synthesized Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites possess the efficient phosphate sorption capacities (SCs) of 40.86, 22.15 and 13.44mg/g than the individual CSBent composite.
28258007	8	48	theme	M	1247:1247	arg1	composites					1256:1265	M@CSBent composites	1247:1265	M@CSBent composites	1247:1265	The mechanism of phosphate removal by M@CSBent composites was governed by ion-exchange, electrostatic attraction and inner sphere complexation.
28258007	5	49	theme	phosphate	988:996	arg1	concentration					998:1010	initial phosphate concentration	980:1010	initial phosphate concentration	980:1010	The systematic study for various adsorption influenced parameters such as agitation time, presence of co-existent anions, solution pH, temperature and initial phosphate concentration has been verified in batch mode.
28258007	5	50	theme	influenced	873:882	arg1	time					913:916	agitation time	903:916	agitation time	903:916	The systematic study for various adsorption influenced parameters such as agitation time, presence of co-existent anions, solution pH, temperature and initial phosphate concentration has been verified in batch mode.
28258007	5	50	theme	influenced	873:882	arg1	pH					960:961	solution pH	951:961	solution pH	951:961	The systematic study for various adsorption influenced parameters such as agitation time, presence of co-existent anions, solution pH, temperature and initial phosphate concentration has been verified in batch mode.
28258007	5	50	theme	influenced	873:882	arg1	concentration					998:1010	initial phosphate concentration	980:1010	initial phosphate concentration	980:1010	The systematic study for various adsorption influenced parameters such as agitation time, presence of co-existent anions, solution pH, temperature and initial phosphate concentration has been verified in batch mode.
28258007	5	50	theme	influenced	873:882	arg1	parameters					884:893	various adsorption influenced parameters	854:893	various adsorption influenced parameters such as agitation time, presence of co-existent anions, solution pH, temperature and initial phosphate concentration	854:1010	The systematic study for various adsorption influenced parameters such as agitation time, presence of co-existent anions, solution pH, temperature and initial phosphate concentration has been verified in batch mode.
28258007	5	50	theme	influenced	873:882	arg1	presence					919:926	presence	919:926	presence of co-existent anions	919:948	The systematic study for various adsorption influenced parameters such as agitation time, presence of co-existent anions, solution pH, temperature and initial phosphate concentration has been verified in batch mode.
28258007	5	50	theme	influenced	873:882	arg1	temperature					964:974	temperature	964:974	temperature	964:974	The systematic study for various adsorption influenced parameters such as agitation time, presence of co-existent anions, solution pH, temperature and initial phosphate concentration has been verified in batch mode.
28258007	9	51	theme	water	1438:1442	arg1	sample					1444:1449	polluted water sample	1429:1449	polluted water sample	1429:1449	This study reveals a feasibility of biocomposites for phosphate uptake from polluted water sample at field situation.
28258007	4	52	theme	synthesized	642:652	arg1	biocomposites					689:701	The synthesized Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites	638:701	The synthesized Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites	638:701	The synthesized Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites possess the efficient phosphate sorption capacities (SCs) of 40.86, 22.15 and 13.44mg/g than the individual CSBent composite.
28258007	0	53	theme	chitosan	48:55	arg1	biocomposites					57:69	chitosan biocomposites	48:69	chitosan biocomposites for phosphate sorption	48:92	Development of multivalent metal ions imprinted chitosan biocomposites for phosphate sorption.
28258007	4	54	theme	13.44mg/g	781:789	arg1	SCs					756:758	SCs	756:758	SCs	756:758	The synthesized Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites possess the efficient phosphate sorption capacities (SCs) of 40.86, 22.15 and 13.44mg/g than the individual CSBent composite.
28258007	4	54	theme	13.44mg/g	781:789	arg1	capacities					744:753	the efficient phosphate sorption capacities	711:753	the efficient phosphate sorption capacities (SCs) of 40.86, 22.15 and 13.44mg/g than the individual CSBent composite	711:826	The synthesized Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites possess the efficient phosphate sorption capacities (SCs) of 40.86, 22.15 and 13.44mg/g than the individual CSBent composite.
28258007	3	55	theme	Ca	600:601	arg1	biocomposites					610:622	Ca@CSBent biocomposites	600:622	Ca@CSBent biocomposites	600:622	To enhance the uptake capacity of CSBent, various multivalent metal ions like Zr4+, Fe3+ and Ca2+ were imprinted on CSBent composites namely Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites respectively.
28258007	4	56	theme	sorption	735:742	arg1	SCs					756:758	SCs	756:758	SCs	756:758	The synthesized Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites possess the efficient phosphate sorption capacities (SCs) of 40.86, 22.15 and 13.44mg/g than the individual CSBent composite.
28258007	4	56	theme	sorption	735:742	arg1	capacities					744:753	the efficient phosphate sorption capacities	711:753	the efficient phosphate sorption capacities (SCs) of 40.86, 22.15 and 13.44mg/g than the individual CSBent composite	711:826	The synthesized Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites possess the efficient phosphate sorption capacities (SCs) of 40.86, 22.15 and 13.44mg/g than the individual CSBent composite.
28258007	2	57	theme	chitosan	320:327	arg1	properties					285:294	the properties	281:294	the properties of bentonite (bent) and chitosan (CS)	281:332	To improve the properties of bentonite (bent) and chitosan (CS), chitosan supported bentonite (CSBent) composites were prepared and utilized for phosphate removal.
28258007	6	58	theme	EDAX	1110:1113	arg1	analysis					1115:1122	EDAX analysis	1110:1122	EDAX analysis	1110:1122	The prepared biocomposites was exemplified by FTIR, TEM, SEM and EDAX analysis.
28258007	8	59	theme	inner	1326:1330	arg1	complexation					1339:1350	inner sphere complexation	1326:1350	inner sphere complexation	1326:1350	The mechanism of phosphate removal by M@CSBent composites was governed by ion-exchange, electrostatic attraction and inner sphere complexation.
28258007	4	60	theme	individual	800:809	arg1	composite					818:826	the individual CSBent composite	796:826	the individual CSBent composite	796:826	The synthesized Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites possess the efficient phosphate sorption capacities (SCs) of 40.86, 22.15 and 13.44mg/g than the individual CSBent composite.
28258007	3	61	theme	@	602:602	arg1	biocomposites					610:622	Ca@CSBent biocomposites	600:622	Ca@CSBent biocomposites	600:622	To enhance the uptake capacity of CSBent, various multivalent metal ions like Zr4+, Fe3+ and Ca2+ were imprinted on CSBent composites namely Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites respectively.
28258007	3	62	theme	Zr	575:576	arg1	CSBent					578:583	Zr@CSBent	575:583	Zr@CSBent	575:583	To enhance the uptake capacity of CSBent, various multivalent metal ions like Zr4+, Fe3+ and Ca2+ were imprinted on CSBent composites namely Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites respectively.
28258007	3	62	theme	Zr	575:576	arg1	composites					557:566	CSBent composites	550:566	CSBent composites namely Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites respectively	550:635	To enhance the uptake capacity of CSBent, various multivalent metal ions like Zr4+, Fe3+ and Ca2+ were imprinted on CSBent composites namely Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites respectively.
28258007	4	63	theme	efficient	715:723	arg1	SCs					756:758	SCs	756:758	SCs	756:758	The synthesized Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites possess the efficient phosphate sorption capacities (SCs) of 40.86, 22.15 and 13.44mg/g than the individual CSBent composite.
28258007	4	63	theme	efficient	715:723	arg1	capacities					744:753	the efficient phosphate sorption capacities	711:753	the efficient phosphate sorption capacities (SCs) of 40.86, 22.15 and 13.44mg/g than the individual CSBent composite	711:826	The synthesized Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites possess the efficient phosphate sorption capacities (SCs) of 40.86, 22.15 and 13.44mg/g than the individual CSBent composite.
28258007	4	64	theme	@	656:656	arg1	CSBent					657:662	Zr@CSBent	654:662	Zr@CSBent	654:662	The synthesized Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites possess the efficient phosphate sorption capacities (SCs) of 40.86, 22.15 and 13.44mg/g than the individual CSBent composite.
28258007	1	65	theme	present	103:109	arg1	work					111:114	this present work	98:114	this present work	98:114	In this present work, to overcome the problem of eutrophication due to phosphate an attempt was made to develop eco-friendly composite materials using chitosan and bentonite.
28258007	1	66	theme	eco-friendly	207:218	arg1	materials					230:238	eco-friendly composite materials	207:238	eco-friendly composite materials	207:238	In this present work, to overcome the problem of eutrophication due to phosphate an attempt was made to develop eco-friendly composite materials using chitosan and bentonite.
28258007	8	67	theme	@	1248:1248	arg1	composites					1256:1265	M@CSBent composites	1247:1265	M@CSBent composites	1247:1265	The mechanism of phosphate removal by M@CSBent composites was governed by ion-exchange, electrostatic attraction and inner sphere complexation.
28258007	9	68	theme	field	1454:1458	arg1	situation					1460:1468	field situation	1454:1468	field situation	1454:1468	This study reveals a feasibility of biocomposites for phosphate uptake from polluted water sample at field situation.
28258007	3	69	theme	@	577:577	arg1	CSBent					578:583	Zr@CSBent	575:583	Zr@CSBent	575:583	To enhance the uptake capacity of CSBent, various multivalent metal ions like Zr4+, Fe3+ and Ca2+ were imprinted on CSBent composites namely Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites respectively.
28258007	3	69	theme	@	577:577	arg1	composites					557:566	CSBent composites	550:566	CSBent composites namely Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites respectively	550:635	To enhance the uptake capacity of CSBent, various multivalent metal ions like Zr4+, Fe3+ and Ca2+ were imprinted on CSBent composites namely Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites respectively.
28258007	3	70	theme	uptake	449:454	arg1	capacity					456:463	the uptake capacity	445:463	the uptake capacity of CSBent	445:473	To enhance the uptake capacity of CSBent, various multivalent metal ions like Zr4+, Fe3+ and Ca2+ were imprinted on CSBent composites namely Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites respectively.
28258007	8	71	theme	removal	1236:1242	arg1	mechanism					1213:1221	The mechanism	1209:1221	The mechanism of phosphate removal by M@CSBent composites	1209:1265	The mechanism of phosphate removal by M@CSBent composites was governed by ion-exchange, electrostatic attraction and inner sphere complexation.
28258007	1	72	theme	composite	220:228	arg1	materials					230:238	eco-friendly composite materials	207:238	eco-friendly composite materials	207:238	In this present work, to overcome the problem of eutrophication due to phosphate an attempt was made to develop eco-friendly composite materials using chitosan and bentonite.
28258007	0	73	theme	phosphate	75:83	arg1	sorption					85:92	phosphate sorption	75:92	phosphate sorption	75:92	Development of multivalent metal ions imprinted chitosan biocomposites for phosphate sorption.
28258007	9	74	theme	biocomposites	1389:1401	arg1	feasibility					1374:1384	a feasibility	1372:1384	a feasibility of biocomposites for phosphate uptake from polluted water sample	1372:1449	This study reveals a feasibility of biocomposites for phosphate uptake from polluted water sample at field situation.
28258007	9	75	theme	polluted	1429:1436	arg1	water					1438:1442	polluted water	1429:1442	polluted water sample	1429:1449	This study reveals a feasibility of biocomposites for phosphate uptake from polluted water sample at field situation.
28258007	2	76	theme	chitosan	335:342	arg1	composites					373:382	chitosan supported bentonite (CSBent) composites	335:382	chitosan supported bentonite (CSBent) composites	335:382	To improve the properties of bentonite (bent) and chitosan (CS), chitosan supported bentonite (CSBent) composites were prepared and utilized for phosphate removal.
28258007	9	77	from	feasibility	1374:1384	arg1	sample					1444:1449	polluted water sample	1429:1449	polluted water sample	1429:1449	This study reveals a feasibility of biocomposites for phosphate uptake from polluted water sample at field situation.
28258007	9	78	from	sample	1444:1449	arg1	feasibility					1374:1384	a feasibility	1372:1384	a feasibility of biocomposites for phosphate uptake from polluted water sample	1372:1449	This study reveals a feasibility of biocomposites for phosphate uptake from polluted water sample at field situation.
28258007	9	78	from	sample	1444:1449	arg1	biocomposites					1389:1401	biocomposites	1389:1401	biocomposites for phosphate uptake from polluted water sample	1389:1449	This study reveals a feasibility of biocomposites for phosphate uptake from polluted water sample at field situation.
28258007	1	79	dep	made	191:194	arg1	overcome					120:127	overcome	120:127	to overcome the problem of eutrophication due to phosphate	117:174	In this present work, to overcome the problem of eutrophication due to phosphate an attempt was made to develop eco-friendly composite materials using chitosan and bentonite.
28258007	3	80	theme	multivalent	484:494	arg1	ions					502:505	various multivalent metal ions	476:505	various multivalent metal ions like Zr4+, Fe3+ and Ca2+	476:530	To enhance the uptake capacity of CSBent, various multivalent metal ions like Zr4+, Fe3+ and Ca2+ were imprinted on CSBent composites namely Zr@CSBent, Fe@CSBent and Ca@CSBent biocomposites respectively.
28258007	7	81	theme	experimental	1129:1140	arg1	data					1142:1145	The experimental data	1125:1145	The experimental data	1125:1145	The experimental data was fitted to various isotherms and thermodynamic parameters.
27334000	5	0	theme	balanced	808:815	arg1	composition					817:827	a more balanced composition	801:827	a more balanced composition of gut microbiota	801:845	Besides, by maintaining a more balanced composition of gut microbiota, dietary fucoidan also significantly reduced the antigen load and the inflammatory response in the host as evidenced by the decreased serum lipopolysaccharide-binding protein levels.
27334000	2	1	theme	microbial	360:368	arg1	structure					370:378	the overall microbial structure	348:378	the overall microbial structure	348:378	However, while previous studies illustrated favorable modulations of gut microbiota by fucoidan, changes in the overall microbial structure remain elusive.
27334000	4	2	theme	bacteria	603:610	arg1	expense					567:573	the expense	563:573	the expense of opportunistic pathogenic bacteria such as Peptococcus	563:630	We found that at the expense of opportunistic pathogenic bacteria such as Peptococcus, the abundance of beneficial bacteria including Lactobacillus and Ruminococcaceae was significantly increased in response to fucoidan treatment.
27334000	2	3	from	changes	337:343	arg1	structure					370:378	the overall microbial structure	348:378	the overall microbial structure	348:378	However, while previous studies illustrated favorable modulations of gut microbiota by fucoidan, changes in the overall microbial structure remain elusive.
27334000	2	4	theme	overall	352:358	arg1	structure					370:378	the overall microbial structure	348:378	the overall microbial structure	348:378	However, while previous studies illustrated favorable modulations of gut microbiota by fucoidan, changes in the overall microbial structure remain elusive.
27334000	4	5	theme	pathogenic	592:601	arg1	bacteria					603:610	opportunistic pathogenic bacteria	578:610	opportunistic pathogenic bacteria such as Peptococcus	578:630	We found that at the expense of opportunistic pathogenic bacteria such as Peptococcus, the abundance of beneficial bacteria including Lactobacillus and Ruminococcaceae was significantly increased in response to fucoidan treatment.
27334000	4	5	theme	pathogenic	592:601	arg1	Peptococcus					620:630	Peptococcus	620:630	Peptococcus	620:630	We found that at the expense of opportunistic pathogenic bacteria such as Peptococcus, the abundance of beneficial bacteria including Lactobacillus and Ruminococcaceae was significantly increased in response to fucoidan treatment.
27334000	0	6	theme	Lactobacillus	85:97	arg1	abundance					72:80	the abundance	68:80	the abundance of Lactobacillus and Ruminococcaceae	68:117	Dietary fucoidan modulates the gut microbiota in mice by increasing the abundance of Lactobacillus and Ruminococcaceae.
27334000	6	7	theme	gut	1096:1098	arg1	modulator					1111:1119	a gut microbiota modulator	1094:1119	a gut microbiota modulator for health promotion and treatment of intestinal dysbiosis	1094:1178	Collectively, our results indicate that fucoidan can be used as a gut microbiota modulator for health promotion and treatment of intestinal dysbiosis.
27334000	6	7	theme	gut	1096:1098	arg1	fucoidan					1070:1077	fucoidan	1070:1077	fucoidan	1070:1077	Collectively, our results indicate that fucoidan can be used as a gut microbiota modulator for health promotion and treatment of intestinal dysbiosis.
27334000	5	8	theme	decreased	971:979	arg1	levels					1022:1027	the decreased serum lipopolysaccharide-binding protein levels	967:1027	the decreased serum lipopolysaccharide-binding protein levels	967:1027	Besides, by maintaining a more balanced composition of gut microbiota, dietary fucoidan also significantly reduced the antigen load and the inflammatory response in the host as evidenced by the decreased serum lipopolysaccharide-binding protein levels.
27334000	0	9	theme	Ruminococcaceae	103:117	arg1	abundance					72:80	the abundance	68:80	the abundance of Lactobacillus and Ruminococcaceae	68:117	Dietary fucoidan modulates the gut microbiota in mice by increasing the abundance of Lactobacillus and Ruminococcaceae.
27334000	4	10	theme	fucoidan	757:764	arg1	treatment					766:774	fucoidan treatment	757:774	fucoidan treatment	757:774	We found that at the expense of opportunistic pathogenic bacteria such as Peptococcus, the abundance of beneficial bacteria including Lactobacillus and Ruminococcaceae was significantly increased in response to fucoidan treatment.
27334000	6	11	theme	microbiota	1100:1109	arg1	modulator					1111:1119	a gut microbiota modulator	1094:1119	a gut microbiota modulator for health promotion and treatment of intestinal dysbiosis	1094:1178	Collectively, our results indicate that fucoidan can be used as a gut microbiota modulator for health promotion and treatment of intestinal dysbiosis.
27334000	6	11	theme	microbiota	1100:1109	arg1	fucoidan					1070:1077	fucoidan	1070:1077	fucoidan	1070:1077	Collectively, our results indicate that fucoidan can be used as a gut microbiota modulator for health promotion and treatment of intestinal dysbiosis.
27334000	2	12	theme	favorable	284:292	arg1	modulations					294:304	favorable modulations	284:304	favorable modulations of gut microbiota by fucoidan	284:334	However, while previous studies illustrated favorable modulations of gut microbiota by fucoidan, changes in the overall microbial structure remain elusive.
27334000	5	13	theme	inflammatory	917:928	arg1	response					930:937	the inflammatory response	913:937	the inflammatory response	913:937	Besides, by maintaining a more balanced composition of gut microbiota, dietary fucoidan also significantly reduced the antigen load and the inflammatory response in the host as evidenced by the decreased serum lipopolysaccharide-binding protein levels.
27334000	3	14	theme	microbiota	437:446	arg1	modulations					418:428	modulations	418:428	modulations of gut microbiota by different fucoidans	418:469	In the present study, modulations of gut microbiota by different fucoidans were studied using high throughput sequencing and bioinformatics analysis.
27334000	0	15	from	microbiota	35:44	arg1	mice					49:52	mice	49:52	mice	49:52	Dietary fucoidan modulates the gut microbiota in mice by increasing the abundance of Lactobacillus and Ruminococcaceae.
27334000	1	16	theme	potential	162:170	arg1	agent					182:186	a potential prebiotic agent	160:186	a potential prebiotic agent for functional food and pharmaceutical development	160:237	Recently, fucoidan has been proposed as a potential prebiotic agent for functional food and pharmaceutical development.
27334000	1	16	theme	potential	162:170	arg1	fucoidan					130:137	fucoidan	130:137	fucoidan	130:137	Recently, fucoidan has been proposed as a potential prebiotic agent for functional food and pharmaceutical development.
27334000	5	17	theme	dietary	848:854	arg1	fucoidan					856:863	dietary fucoidan	848:863	dietary fucoidan	848:863	Besides, by maintaining a more balanced composition of gut microbiota, dietary fucoidan also significantly reduced the antigen load and the inflammatory response in the host as evidenced by the decreased serum lipopolysaccharide-binding protein levels.
27334000	6	18	used	used	1086:1089	arg2	modulator					1111:1119	a gut microbiota modulator	1094:1119	a gut microbiota modulator for health promotion and treatment of intestinal dysbiosis	1094:1178	Collectively, our results indicate that fucoidan can be used as a gut microbiota modulator for health promotion and treatment of intestinal dysbiosis.
27334000	6	18	used	used	1086:1089	arg2	fucoidan					1070:1077	fucoidan	1070:1077	fucoidan	1070:1077	Collectively, our results indicate that fucoidan can be used as a gut microbiota modulator for health promotion and treatment of intestinal dysbiosis.
27334000	5	19	theme	serum	981:985	arg1	levels					1022:1027	the decreased serum lipopolysaccharide-binding protein levels	967:1027	the decreased serum lipopolysaccharide-binding protein levels	967:1027	Besides, by maintaining a more balanced composition of gut microbiota, dietary fucoidan also significantly reduced the antigen load and the inflammatory response in the host as evidenced by the decreased serum lipopolysaccharide-binding protein levels.
27334000	6	20	theme	health	1125:1130	arg1	promotion					1132:1140	health promotion	1125:1140	health promotion	1125:1140	Collectively, our results indicate that fucoidan can be used as a gut microbiota modulator for health promotion and treatment of intestinal dysbiosis.
27334000	1	21	theme	prebiotic	172:180	arg1	agent					182:186	a potential prebiotic agent	160:186	a potential prebiotic agent for functional food and pharmaceutical development	160:237	Recently, fucoidan has been proposed as a potential prebiotic agent for functional food and pharmaceutical development.
27334000	1	21	theme	prebiotic	172:180	arg1	fucoidan					130:137	fucoidan	130:137	fucoidan	130:137	Recently, fucoidan has been proposed as a potential prebiotic agent for functional food and pharmaceutical development.
27334000	0	22	theme	Dietary	0:6	arg1	fucoidan					8:15	Dietary fucoidan	0:15	Dietary fucoidan	0:15	Dietary fucoidan modulates the gut microbiota in mice by increasing the abundance of Lactobacillus and Ruminococcaceae.
27334000	5	23	theme	lipopolysaccharide-binding	987:1012	arg1	levels					1022:1027	the decreased serum lipopolysaccharide-binding protein levels	967:1027	the decreased serum lipopolysaccharide-binding protein levels	967:1027	Besides, by maintaining a more balanced composition of gut microbiota, dietary fucoidan also significantly reduced the antigen load and the inflammatory response in the host as evidenced by the decreased serum lipopolysaccharide-binding protein levels.
27334000	3	24	theme	gut	433:435	arg1	microbiota					437:446	gut microbiota	433:446	gut microbiota	433:446	In the present study, modulations of gut microbiota by different fucoidans were studied using high throughput sequencing and bioinformatics analysis.
27334000	2	25	theme	microbiota	313:322	arg1	modulations					294:304	favorable modulations	284:304	favorable modulations of gut microbiota by fucoidan	284:334	However, while previous studies illustrated favorable modulations of gut microbiota by fucoidan, changes in the overall microbial structure remain elusive.
27334000	4	26	theme	beneficial	650:659	arg1	bacteria					661:668	beneficial bacteria	650:668	beneficial bacteria including Lactobacillus and Ruminococcaceae	650:712	We found that at the expense of opportunistic pathogenic bacteria such as Peptococcus, the abundance of beneficial bacteria including Lactobacillus and Ruminococcaceae was significantly increased in response to fucoidan treatment.
27334000	4	26	theme	beneficial	650:659	arg1	Ruminococcaceae					698:712	Ruminococcaceae	698:712	Ruminococcaceae	698:712	We found that at the expense of opportunistic pathogenic bacteria such as Peptococcus, the abundance of beneficial bacteria including Lactobacillus and Ruminococcaceae was significantly increased in response to fucoidan treatment.
27334000	4	26	theme	beneficial	650:659	arg1	Lactobacillus					680:692	Lactobacillus	680:692	Lactobacillus	680:692	We found that at the expense of opportunistic pathogenic bacteria such as Peptococcus, the abundance of beneficial bacteria including Lactobacillus and Ruminococcaceae was significantly increased in response to fucoidan treatment.
27334000	4	27	theme	opportunistic	578:590	arg1	bacteria					603:610	opportunistic pathogenic bacteria	578:610	opportunistic pathogenic bacteria such as Peptococcus	578:630	We found that at the expense of opportunistic pathogenic bacteria such as Peptococcus, the abundance of beneficial bacteria including Lactobacillus and Ruminococcaceae was significantly increased in response to fucoidan treatment.
27334000	4	27	theme	opportunistic	578:590	arg1	Peptococcus					620:630	Peptococcus	620:630	Peptococcus	620:630	We found that at the expense of opportunistic pathogenic bacteria such as Peptococcus, the abundance of beneficial bacteria including Lactobacillus and Ruminococcaceae was significantly increased in response to fucoidan treatment.
27334000	2	28	theme	gut	309:311	arg1	microbiota					313:322	gut microbiota	309:322	gut microbiota	309:322	However, while previous studies illustrated favorable modulations of gut microbiota by fucoidan, changes in the overall microbial structure remain elusive.
27334000	1	29	theme	functional	192:201	arg1	food					203:206	functional food	192:206	functional food	192:206	Recently, fucoidan has been proposed as a potential prebiotic agent for functional food and pharmaceutical development.
27334000	5	30	theme	antigen	896:902	arg1	load					904:907	the antigen load	892:907	the antigen load	892:907	Besides, by maintaining a more balanced composition of gut microbiota, dietary fucoidan also significantly reduced the antigen load and the inflammatory response in the host as evidenced by the decreased serum lipopolysaccharide-binding protein levels.
27334000	3	31	theme	different	451:459	arg1	fucoidans					461:469	different fucoidans	451:469	different fucoidans	451:469	In the present study, modulations of gut microbiota by different fucoidans were studied using high throughput sequencing and bioinformatics analysis.
27334000	0	32	theme	gut	31:33	arg1	microbiota					35:44	the gut microbiota	27:44	the gut microbiota in mice	27:52	Dietary fucoidan modulates the gut microbiota in mice by increasing the abundance of Lactobacillus and Ruminococcaceae.
27334000	5	33	theme	gut	832:834	arg1	microbiota					836:845	gut microbiota	832:845	gut microbiota	832:845	Besides, by maintaining a more balanced composition of gut microbiota, dietary fucoidan also significantly reduced the antigen load and the inflammatory response in the host as evidenced by the decreased serum lipopolysaccharide-binding protein levels.
27334000	4	34	theme	bacteria	661:668	arg1	abundance					637:645	the abundance	633:645	the abundance of beneficial bacteria including Lactobacillus and Ruminococcaceae	633:712	We found that at the expense of opportunistic pathogenic bacteria such as Peptococcus, the abundance of beneficial bacteria including Lactobacillus and Ruminococcaceae was significantly increased in response to fucoidan treatment.
27334000	3	35	theme	present	403:409	arg1	study					411:415	the present study	399:415	the present study	399:415	In the present study, modulations of gut microbiota by different fucoidans were studied using high throughput sequencing and bioinformatics analysis.
27334000	5	36	from	load	904:907	arg1	host					946:949	the host	942:949	the host	942:949	Besides, by maintaining a more balanced composition of gut microbiota, dietary fucoidan also significantly reduced the antigen load and the inflammatory response in the host as evidenced by the decreased serum lipopolysaccharide-binding protein levels.
27334000	3	37	theme	throughput	495:504	arg1	sequencing					506:515	high throughput sequencing	490:515	high throughput sequencing	490:515	In the present study, modulations of gut microbiota by different fucoidans were studied using high throughput sequencing and bioinformatics analysis.
27334000	3	38	theme	bioinformatics	521:534	arg1	analysis					536:543	bioinformatics analysis	521:543	bioinformatics analysis	521:543	In the present study, modulations of gut microbiota by different fucoidans were studied using high throughput sequencing and bioinformatics analysis.
27334000	5	39	theme	protein	1014:1020	arg1	levels					1022:1027	the decreased serum lipopolysaccharide-binding protein levels	967:1027	the decreased serum lipopolysaccharide-binding protein levels	967:1027	Besides, by maintaining a more balanced composition of gut microbiota, dietary fucoidan also significantly reduced the antigen load and the inflammatory response in the host as evidenced by the decreased serum lipopolysaccharide-binding protein levels.
27334000	1	40	theme	pharmaceutical	212:225	arg1	development					227:237	pharmaceutical development	212:237	pharmaceutical development	212:237	Recently, fucoidan has been proposed as a potential prebiotic agent for functional food and pharmaceutical development.
27334000	2	41	theme	previous	255:262	arg1	studies					264:270	previous studies	255:270	previous studies	255:270	However, while previous studies illustrated favorable modulations of gut microbiota by fucoidan, changes in the overall microbial structure remain elusive.
27334000	3	42	theme	high	490:493	arg1	sequencing					506:515	high throughput sequencing	490:515	high throughput sequencing	490:515	In the present study, modulations of gut microbiota by different fucoidans were studied using high throughput sequencing and bioinformatics analysis.
27334000	5	43	from	response	930:937	arg1	host					946:949	the host	942:949	the host	942:949	Besides, by maintaining a more balanced composition of gut microbiota, dietary fucoidan also significantly reduced the antigen load and the inflammatory response in the host as evidenced by the decreased serum lipopolysaccharide-binding protein levels.
27334000	6	44	theme	dysbiosis	1170:1178	arg1	treatment					1146:1154	treatment	1146:1154	treatment of intestinal dysbiosis	1146:1178	Collectively, our results indicate that fucoidan can be used as a gut microbiota modulator for health promotion and treatment of intestinal dysbiosis.
27334000	6	44	theme	dysbiosis	1170:1178	arg1	promotion					1132:1140	health promotion	1125:1140	health promotion	1125:1140	Collectively, our results indicate that fucoidan can be used as a gut microbiota modulator for health promotion and treatment of intestinal dysbiosis.
27334000	5	45	theme	microbiota	836:845	arg1	composition					817:827	a more balanced composition	801:827	a more balanced composition of gut microbiota	801:845	Besides, by maintaining a more balanced composition of gut microbiota, dietary fucoidan also significantly reduced the antigen load and the inflammatory response in the host as evidenced by the decreased serum lipopolysaccharide-binding protein levels.
27334000	6	46	theme	intestinal	1159:1168	arg1	dysbiosis					1170:1178	intestinal dysbiosis	1159:1178	intestinal dysbiosis	1159:1178	Collectively, our results indicate that fucoidan can be used as a gut microbiota modulator for health promotion and treatment of intestinal dysbiosis.
27688023	8	0	theme	enzyme-substrate	1122:1137	arg1	structure					1147:1155	the previously determined enzyme-substrate complex structure	1096:1155	the previously determined enzyme-substrate complex structure	1096:1155	Comparison between these structures and the previously determined enzyme-substrate complex structure reveals that the glucose pyranose ring might be distorted immediately after nucleophilic attack by a water molecule.
27688023	6	1	located	found	827:831	arg1	sites					847:851	the active sites	836:851	the active sites in both structures	836:870	Because glucose and lactose molecules are found at the active sites in both structures, it is possible that these structures mimic the enzyme-product complex of YgjK.
27688023	6	1	located	found	827:831	arg2	molecules					813:821	glucose and lactose molecules	793:821	glucose and lactose molecules	793:821	Because glucose and lactose molecules are found at the active sites in both structures, it is possible that these structures mimic the enzyme-product complex of YgjK.
27688023	1	2	theme	hydrolytic	240:249	arg1	mechanisms					251:260	their hydrolytic mechanisms	234:260	their hydrolytic mechanisms	234:260	Glycoside hydrolases are divided into two groups, known as inverting and retaining enzymes, based on their hydrolytic mechanisms.
27688023	5	3	theme	1.8-	743:746	arg1	resolutions					758:768	1.8- and 2.1-Å resolutions	743:768	1.8- and 2.1-Å resolutions	743:768	Here, we determined the crystal structures of D324N and E727A soaked with a mixture of glucose and lactose at 1.8- and 2.1-Å resolutions, respectively.
27688023	7	4	theme	skew-boat	1032:1040	arg1	conformation					1042:1053	an unusual 1S3 skew-boat conformation	1017:1053	an unusual 1S3 skew-boat conformation	1017:1053	A glucose molecule found at subsite -1 in both structures adopts an unusual 1S3 skew-boat conformation.
27688023	6	5	theme	glucose	793:799	arg1	molecules					813:821	glucose and lactose molecules	793:821	glucose and lactose molecules	793:821	Because glucose and lactose molecules are found at the active sites in both structures, it is possible that these structures mimic the enzyme-product complex of YgjK.
27688023	4	6	theme	β-glucosyl	569:578	arg1	donor					589:593	a β-glucosyl fluoride donor	567:593	a β-glucosyl fluoride donor	567:593	Two constructed glycosynthase mutants, D324N and E727A, which catalyze the transfer of a β-glucosyl fluoride donor to galactose, lactose, and melibiose.
27688023	6	7	from	sites	847:851	arg1	structures					861:870	both structures	856:870	both structures	856:870	Because glucose and lactose molecules are found at the active sites in both structures, it is possible that these structures mimic the enzyme-product complex of YgjK.
27688023	9	8	theme	first	1305:1309	arg1	complex					1326:1332	the first enzyme-product complex	1301:1332	the first enzyme-product complex for the GH63 family	1301:1352	These structures represent the first enzyme-product complex for the GH63 family, as well as the structurally-related glycosidases, and it may provide insight into the catalytic mechanism of these enzymes.
27688023	6	9	theme	YgjK	946:949	arg1	complex					935:941	the enzyme-product complex	916:941	the enzyme-product complex of YgjK	916:949	Because glucose and lactose molecules are found at the active sites in both structures, it is possible that these structures mimic the enzyme-product complex of YgjK.
27688023	4	10	theme	donor	589:593	arg1	transfer					555:562	the transfer	551:562	the transfer of a β-glucosyl fluoride donor to galactose, lactose, and melibiose	551:630	Two constructed glycosynthase mutants, D324N and E727A, which catalyze the transfer of a β-glucosyl fluoride donor to galactose, lactose, and melibiose.
27688023	7	11	located	found	971:975	arg1	structures					999:1008	both structures	994:1008	both structures	994:1008	A glucose molecule found at subsite -1 in both structures adopts an unusual 1S3 skew-boat conformation.
27688023	7	11	located	found	971:975	arg2	molecule					962:969	A glucose molecule	952:969	A glucose molecule found at subsite -1 in both structures	952:1008	A glucose molecule found at subsite -1 in both structures adopts an unusual 1S3 skew-boat conformation.
27688023	7	11	located	found	971:975	arg1	subsite					980:986	subsite -1	980:989	subsite -1	980:989	A glucose molecule found at subsite -1 in both structures adopts an unusual 1S3 skew-boat conformation.
27688023	9	12	theme	enzyme-product	1311:1324	arg1	complex					1326:1332	the first enzyme-product complex	1301:1332	the first enzyme-product complex for the GH63 family	1301:1352	These structures represent the first enzyme-product complex for the GH63 family, as well as the structurally-related glycosidases, and it may provide insight into the catalytic mechanism of these enzymes.
27688023	8	13	theme	water	1258:1262	arg1	molecule					1264:1271	a water molecule	1256:1271	a water molecule	1256:1271	Comparison between these structures and the previously determined enzyme-substrate complex structure reveals that the glucose pyranose ring might be distorted immediately after nucleophilic attack by a water molecule.
27688023	9	14	theme	catalytic	1441:1449	arg1	mechanism					1451:1459	the catalytic mechanism	1437:1459	the catalytic mechanism of these enzymes	1437:1476	These structures represent the first enzyme-product complex for the GH63 family, as well as the structurally-related glycosidases, and it may provide insight into the catalytic mechanism of these enzymes.
27688023	8	15	theme	nucleophilic	1233:1244	arg1	attack					1246:1251	nucleophilic attack	1233:1251	nucleophilic attack by a water molecule	1233:1271	Comparison between these structures and the previously determined enzyme-substrate complex structure reveals that the glucose pyranose ring might be distorted immediately after nucleophilic attack by a water molecule.
27688023	5	16	theme	glucose	720:726	arg1	mixture					709:715	a mixture	707:715	a mixture of glucose and lactose	707:738	Here, we determined the crystal structures of D324N and E727A soaked with a mixture of glucose and lactose at 1.8- and 2.1-Å resolutions, respectively.
27688023	4	17	theme	glycosynthase	496:508	arg1	E727A					529:533	E727A	529:533	E727A	529:533	Two constructed glycosynthase mutants, D324N and E727A, which catalyze the transfer of a β-glucosyl fluoride donor to galactose, lactose, and melibiose.
27688023	4	17	theme	glycosynthase	496:508	arg1	D324N					519:523	D324N	519:523	D324N	519:523	Two constructed glycosynthase mutants, D324N and E727A, which catalyze the transfer of a β-glucosyl fluoride donor to galactose, lactose, and melibiose.
27688023	4	17	theme	glycosynthase	496:508	arg1	mutants					510:516	glycosynthase mutants	496:516	glycosynthase mutants	496:516	Two constructed glycosynthase mutants, D324N and E727A, which catalyze the transfer of a β-glucosyl fluoride donor to galactose, lactose, and melibiose.
27688023	0	18	theme	complex	40:46	arg1	structure					8:16	Crystal structure	0:16	Crystal structure of the enzyme-product complex	0:46	Crystal structure of the enzyme-product complex reveals sugar ring distortion during catalysis by family 63 inverting α-glycosidase.
27688023	7	19	theme	1S3	1028:1030	arg1	conformation					1042:1053	an unusual 1S3 skew-boat conformation	1017:1053	an unusual 1S3 skew-boat conformation	1017:1053	A glucose molecule found at subsite -1 in both structures adopts an unusual 1S3 skew-boat conformation.
27688023	2	20	theme	hydrolase	273:281	arg1	family					283:288	Glycoside hydrolase family 63	263:291	Glycoside hydrolase family 63 (GH63)	263:298	Glycoside hydrolase family 63 (GH63) is composed of inverting α-glycosidases, which act mainly on α-glucosides.
27688023	2	20	theme	hydrolase	273:281	arg1	GH63					294:297	GH63	294:297	GH63	294:297	Glycoside hydrolase family 63 (GH63) is composed of inverting α-glycosidases, which act mainly on α-glucosides.
27688023	4	21	theme	fluoride	580:587	arg1	donor					589:593	a β-glucosyl fluoride donor	567:593	a β-glucosyl fluoride donor	567:593	Two constructed glycosynthase mutants, D324N and E727A, which catalyze the transfer of a β-glucosyl fluoride donor to galactose, lactose, and melibiose.
27688023	8	22	theme	determined	1111:1120	arg1	structure					1147:1155	the previously determined enzyme-substrate complex structure	1096:1155	the previously determined enzyme-substrate complex structure	1096:1155	Comparison between these structures and the previously determined enzyme-substrate complex structure reveals that the glucose pyranose ring might be distorted immediately after nucleophilic attack by a water molecule.
27688023	3	23	dep	Escherichia	400:410	arg1	coli					412:415	coli	412:415	coli	412:415	We previously found that Escherichia coli GH63 enzyme, YgjK, can hydrolyze 2-O-α-d-glucosyl-d-galactose.
27688023	3	24	theme	GH63	417:420	arg1	YgjK					430:433	YgjK	430:433	YgjK	430:433	We previously found that Escherichia coli GH63 enzyme, YgjK, can hydrolyze 2-O-α-d-glucosyl-d-galactose.
27688023	3	24	theme	GH63	417:420	arg1	enzyme					422:427	Escherichia coli GH63 enzyme	400:427	Escherichia coli GH63 enzyme	400:427	We previously found that Escherichia coli GH63 enzyme, YgjK, can hydrolyze 2-O-α-d-glucosyl-d-galactose.
27688023	0	25	theme	Crystal	0:6	arg1	structure					8:16	Crystal structure	0:16	Crystal structure of the enzyme-product complex	0:46	Crystal structure of the enzyme-product complex reveals sugar ring distortion during catalysis by family 63 inverting α-glycosidase.
27688023	9	26	theme	GH63	1342:1345	arg1	family					1347:1352	the GH63 family	1338:1352	the GH63 family	1338:1352	These structures represent the first enzyme-product complex for the GH63 family, as well as the structurally-related glycosidases, and it may provide insight into the catalytic mechanism of these enzymes.
27688023	7	27	theme	unusual	1020:1026	arg1	conformation					1042:1053	an unusual 1S3 skew-boat conformation	1017:1053	an unusual 1S3 skew-boat conformation	1017:1053	A glucose molecule found at subsite -1 in both structures adopts an unusual 1S3 skew-boat conformation.
27688023	1	28	theme	inverting	192:200	arg1	enzymes					216:222	inverting and retaining enzymes	192:222	inverting and retaining enzymes	192:222	Glycoside hydrolases are divided into two groups, known as inverting and retaining enzymes, based on their hydrolytic mechanisms.
27688023	8	29	theme	pyranose	1182:1189	arg1	ring					1191:1194	the glucose pyranose ring	1170:1194	the glucose pyranose ring	1170:1194	Comparison between these structures and the previously determined enzyme-substrate complex structure reveals that the glucose pyranose ring might be distorted immediately after nucleophilic attack by a water molecule.
27688023	6	30	theme	enzyme-product	920:933	arg1	complex					935:941	the enzyme-product complex	916:941	the enzyme-product complex of YgjK	916:949	Because glucose and lactose molecules are found at the active sites in both structures, it is possible that these structures mimic the enzyme-product complex of YgjK.
27688023	5	31	theme	E727A	689:693	arg1	structures					665:674	the crystal structures	653:674	the crystal structures of D324N and E727A soaked with a mixture of glucose and lactose at 1.8- and 2.1-Å resolutions	653:768	Here, we determined the crystal structures of D324N and E727A soaked with a mixture of glucose and lactose at 1.8- and 2.1-Å resolutions, respectively.
27688023	6	32	theme	active	840:845	arg1	sites					847:851	the active sites	836:851	the active sites in both structures	836:870	Because glucose and lactose molecules are found at the active sites in both structures, it is possible that these structures mimic the enzyme-product complex of YgjK.
27688023	3	33	theme	Escherichia	400:410	arg1	YgjK					430:433	YgjK	430:433	YgjK	430:433	We previously found that Escherichia coli GH63 enzyme, YgjK, can hydrolyze 2-O-α-d-glucosyl-d-galactose.
27688023	3	33	theme	Escherichia	400:410	arg1	enzyme					422:427	Escherichia coli GH63 enzyme	400:427	Escherichia coli GH63 enzyme	400:427	We previously found that Escherichia coli GH63 enzyme, YgjK, can hydrolyze 2-O-α-d-glucosyl-d-galactose.
27688023	9	34	theme	enzymes	1470:1476	arg1	mechanism					1451:1459	the catalytic mechanism	1437:1459	the catalytic mechanism of these enzymes	1437:1476	These structures represent the first enzyme-product complex for the GH63 family, as well as the structurally-related glycosidases, and it may provide insight into the catalytic mechanism of these enzymes.
27688023	1	35	theme	retaining	206:214	arg1	enzymes					216:222	inverting and retaining enzymes	192:222	inverting and retaining enzymes	192:222	Glycoside hydrolases are divided into two groups, known as inverting and retaining enzymes, based on their hydrolytic mechanisms.
27688023	0	36	theme	enzyme-product	25:38	arg1	complex					40:46	the enzyme-product complex	21:46	the enzyme-product complex	21:46	Crystal structure of the enzyme-product complex reveals sugar ring distortion during catalysis by family 63 inverting α-glycosidase.
27688023	5	37	theme	2.1-Å	752:756	arg1	resolutions					758:768	1.8- and 2.1-Å resolutions	743:768	1.8- and 2.1-Å resolutions	743:768	Here, we determined the crystal structures of D324N and E727A soaked with a mixture of glucose and lactose at 1.8- and 2.1-Å resolutions, respectively.
27688023	0	38	theme	sugar	56:60	arg1	distortion					67:76	sugar ring distortion	56:76	sugar ring distortion	56:76	Crystal structure of the enzyme-product complex reveals sugar ring distortion during catalysis by family 63 inverting α-glycosidase.
27688023	5	39	theme	crystal	657:663	arg1	structures					665:674	the crystal structures	653:674	the crystal structures of D324N and E727A soaked with a mixture of glucose and lactose at 1.8- and 2.1-Å resolutions	653:768	Here, we determined the crystal structures of D324N and E727A soaked with a mixture of glucose and lactose at 1.8- and 2.1-Å resolutions, respectively.
27688023	5	40	theme	lactose	732:738	arg1	mixture					709:715	a mixture	707:715	a mixture of glucose and lactose	707:738	Here, we determined the crystal structures of D324N and E727A soaked with a mixture of glucose and lactose at 1.8- and 2.1-Å resolutions, respectively.
27688023	2	41	theme	Glycoside	263:271	arg1	family					283:288	Glycoside hydrolase family 63	263:291	Glycoside hydrolase family 63 (GH63)	263:298	Glycoside hydrolase family 63 (GH63) is composed of inverting α-glycosidases, which act mainly on α-glucosides.
27688023	2	41	theme	Glycoside	263:271	arg1	GH63					294:297	GH63	294:297	GH63	294:297	Glycoside hydrolase family 63 (GH63) is composed of inverting α-glycosidases, which act mainly on α-glucosides.
27688023	1	42	theme	Glycoside	133:141	arg1	hydrolases					143:152	Glycoside hydrolases	133:152	Glycoside hydrolases	133:152	Glycoside hydrolases are divided into two groups, known as inverting and retaining enzymes, based on their hydrolytic mechanisms.
27688023	9	43	theme	structurally-related	1370:1389	arg1	glycosidases					1391:1402	the structurally-related glycosidases	1366:1402	the structurally-related glycosidases	1366:1402	These structures represent the first enzyme-product complex for the GH63 family, as well as the structurally-related glycosidases, and it may provide insight into the catalytic mechanism of these enzymes.
27688023	5	44	theme	D324N	679:683	arg1	structures					665:674	the crystal structures	653:674	the crystal structures of D324N and E727A soaked with a mixture of glucose and lactose at 1.8- and 2.1-Å resolutions	653:768	Here, we determined the crystal structures of D324N and E727A soaked with a mixture of glucose and lactose at 1.8- and 2.1-Å resolutions, respectively.
27688023	6	45	theme	lactose	805:811	arg1	molecules					813:821	glucose and lactose molecules	793:821	glucose and lactose molecules	793:821	Because glucose and lactose molecules are found at the active sites in both structures, it is possible that these structures mimic the enzyme-product complex of YgjK.
27688023	2	46	theme	inverting	315:323	arg1	α-glycosidases					325:338	inverting α-glycosidases	315:338	inverting α-glycosidases	315:338	Glycoside hydrolase family 63 (GH63) is composed of inverting α-glycosidases, which act mainly on α-glucosides.
27688023	7	47	theme	glucose	954:960	arg1	molecule					962:969	A glucose molecule	952:969	A glucose molecule found at subsite -1 in both structures	952:1008	A glucose molecule found at subsite -1 in both structures adopts an unusual 1S3 skew-boat conformation.
27688023	0	48	theme	ring	62:65	arg1	distortion					67:76	sugar ring distortion	56:76	sugar ring distortion	56:76	Crystal structure of the enzyme-product complex reveals sugar ring distortion during catalysis by family 63 inverting α-glycosidase.
27688023	8	49	theme	glucose	1174:1180	arg1	ring					1191:1194	the glucose pyranose ring	1170:1194	the glucose pyranose ring	1170:1194	Comparison between these structures and the previously determined enzyme-substrate complex structure reveals that the glucose pyranose ring might be distorted immediately after nucleophilic attack by a water molecule.
27688023	8	50	theme	complex	1139:1145	arg1	structure					1147:1155	the previously determined enzyme-substrate complex structure	1096:1155	the previously determined enzyme-substrate complex structure	1096:1155	Comparison between these structures and the previously determined enzyme-substrate complex structure reveals that the glucose pyranose ring might be distorted immediately after nucleophilic attack by a water molecule.
24183808	8	0	theme	higher	1083:1088	arg1	dispersibility					1090:1103	higher dispersibility	1083:1103	higher dispersibility	1083:1103	Furthermore, in the aqueous phase, our novel composites exhibited higher dispersibility compared with pristine MWCNTs.
24183808	7	1	theme	transmission	930:941	arg1	TEM					964:966	TEM	964:966	TEM	964:966	Moreover, the presence of polyHEMA on the surface of the CS functionalized carbon nanotubes was confirmed by field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and thermo gravimetric analysis (TGA) analyses.
24183808	7	1	theme	transmission	930:941	arg1	microscopy					952:961	transmission electron microscopy	930:961	transmission electron microscopy (TEM)	930:967	Moreover, the presence of polyHEMA on the surface of the CS functionalized carbon nanotubes was confirmed by field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and thermo gravimetric analysis (TGA) analyses.
24183808	7	2	theme	carbon	843:848	arg1	nanotubes					850:858	the CS functionalized carbon nanotubes	821:858	the CS functionalized carbon nanotubes	821:858	Moreover, the presence of polyHEMA on the surface of the CS functionalized carbon nanotubes was confirmed by field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and thermo gravimetric analysis (TGA) analyses.
24183808	1	3	theme	MWCNT	205:209	arg1	composites					212:221	chitosan (CS) functionalized multi-walled carbon nanotube (MWCNT) composites	146:221	chitosan (CS) functionalized multi-walled carbon nanotube (MWCNT) composites with a biomedically important polymer, poly-2-hydroxyethyl methacrylate (polyHEMA),	146:305	We report a simple method for engineering chitosan (CS) functionalized multi-walled carbon nanotube (MWCNT) composites with a biomedically important polymer, poly-2-hydroxyethyl methacrylate (polyHEMA), by chemical grafting HEMA monomers via free radical polymerization.
24183808	1	4	theme	simple	116:121	arg1	method					123:128	a simple method	114:128	a simple method for engineering chitosan (CS) functionalized multi-walled carbon nanotube (MWCNT) composites with a biomedically important polymer, poly-2-hydroxyethyl methacrylate (polyHEMA), by chemical grafting HEMA monomers via free radical polymerization	114:372	We report a simple method for engineering chitosan (CS) functionalized multi-walled carbon nanotube (MWCNT) composites with a biomedically important polymer, poly-2-hydroxyethyl methacrylate (polyHEMA), by chemical grafting HEMA monomers via free radical polymerization.
24183808	9	5	from	applications	1320:1331	arg1	delivery					1341:1348	drug delivery	1336:1348	drug delivery	1336:1348	Considering the biomedical importance of polyHEMA and CS polymers, we expect these materials to be useful in the pharmaceutical industry as novel biomaterial composites with potential applications in drug delivery.
24183808	9	6	theme	CS	1190:1191	arg1	polymers					1193:1200	polyHEMA and CS polymers	1177:1200	polyHEMA and CS polymers	1177:1200	Considering the biomedical importance of polyHEMA and CS polymers, we expect these materials to be useful in the pharmaceutical industry as novel biomaterial composites with potential applications in drug delivery.
24183808	6	7	dep	Fourier	720:726	arg1	transform					728:736	transform	728:736	transform infrared (FTIR) spectroscopy	728:765	Composite synthesis was confirmed by Fourier transform infrared (FTIR) spectroscopy.
24183808	1	8	theme	chemical	310:317	arg1	monomers					333:340	chemical grafting HEMA monomers	310:340	chemical grafting HEMA monomers	310:340	We report a simple method for engineering chitosan (CS) functionalized multi-walled carbon nanotube (MWCNT) composites with a biomedically important polymer, poly-2-hydroxyethyl methacrylate (polyHEMA), by chemical grafting HEMA monomers via free radical polymerization.
24183808	5	9	theme	final	617:621	arg1	step					623:626	The final step	613:626	The final step	613:626	The final step involved free radical polymerization of HEMA monomers.
24183808	9	10	theme	polyHEMA	1177:1184	arg1	polymers					1193:1200	polyHEMA and CS polymers	1177:1200	polyHEMA and CS polymers	1177:1200	Considering the biomedical importance of polyHEMA and CS polymers, we expect these materials to be useful in the pharmaceutical industry as novel biomaterial composites with potential applications in drug delivery.
24183808	8	11	theme	pristine	1119:1126	arg1	MWCNTs					1128:1133	pristine MWCNTs	1119:1133	pristine MWCNTs	1119:1133	Furthermore, in the aqueous phase, our novel composites exhibited higher dispersibility compared with pristine MWCNTs.
24183808	1	12	theme	grafting	319:326	arg1	monomers					333:340	chemical grafting HEMA monomers	310:340	chemical grafting HEMA monomers	310:340	We report a simple method for engineering chitosan (CS) functionalized multi-walled carbon nanotube (MWCNT) composites with a biomedically important polymer, poly-2-hydroxyethyl methacrylate (polyHEMA), by chemical grafting HEMA monomers via free radical polymerization.
24183808	6	13	dep	infrared	738:745	arg1	FTIR					748:751	FTIR	748:751	FTIR	748:751	Composite synthesis was confirmed by Fourier transform infrared (FTIR) spectroscopy.
24183808	1	14	theme	HEMA	328:331	arg1	monomers					333:340	chemical grafting HEMA monomers	310:340	chemical grafting HEMA monomers	310:340	We report a simple method for engineering chitosan (CS) functionalized multi-walled carbon nanotube (MWCNT) composites with a biomedically important polymer, poly-2-hydroxyethyl methacrylate (polyHEMA), by chemical grafting HEMA monomers via free radical polymerization.
24183808	9	15	from	useful	1235:1240	arg1	industry					1264:1271	the pharmaceutical industry	1245:1271	the pharmaceutical industry	1245:1271	Considering the biomedical importance of polyHEMA and CS polymers, we expect these materials to be useful in the pharmaceutical industry as novel biomaterial composites with potential applications in drug delivery.
24183808	7	16	theme	scanning	892:899	arg1	FESEM					922:926	FESEM	922:926	FESEM	922:926	Moreover, the presence of polyHEMA on the surface of the CS functionalized carbon nanotubes was confirmed by field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and thermo gravimetric analysis (TGA) analyses.
24183808	7	16	theme	scanning	892:899	arg1	microscopy					910:919	field emission scanning electron microscopy	877:919	field emission scanning electron microscopy (FESEM)	877:927	Moreover, the presence of polyHEMA on the surface of the CS functionalized carbon nanotubes was confirmed by field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and thermo gravimetric analysis (TGA) analyses.
24183808	0	17	from	methods	95:101	arg1	grafting					80:87	grafting	80:87	grafting	80:87	Grafting chitosan and polyHEMA on carbon nanotubes surfaces: "grafting to" and "grafting from" methods.
24183808	9	18	from	industry	1264:1271	arg1	useful					1235:1240	useful	1235:1240	useful	1235:1240	Considering the biomedical importance of polyHEMA and CS polymers, we expect these materials to be useful in the pharmaceutical industry as novel biomaterial composites with potential applications in drug delivery.
24183808	9	19	with	composites	1294:1303	arg1	applications					1320:1331	potential applications	1310:1331	potential applications in drug delivery	1310:1348	Considering the biomedical importance of polyHEMA and CS polymers, we expect these materials to be useful in the pharmaceutical industry as novel biomaterial composites with potential applications in drug delivery.
24183808	7	20	theme	electron	901:908	arg1	FESEM					922:926	FESEM	922:926	FESEM	922:926	Moreover, the presence of polyHEMA on the surface of the CS functionalized carbon nanotubes was confirmed by field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and thermo gravimetric analysis (TGA) analyses.
24183808	7	20	theme	electron	901:908	arg1	microscopy					910:919	field emission scanning electron microscopy	877:919	field emission scanning electron microscopy (FESEM)	877:927	Moreover, the presence of polyHEMA on the surface of the CS functionalized carbon nanotubes was confirmed by field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and thermo gravimetric analysis (TGA) analyses.
24183808	9	21	theme	pharmaceutical	1249:1262	arg1	industry					1264:1271	the pharmaceutical industry	1245:1271	the pharmaceutical industry	1245:1271	Considering the biomedical importance of polyHEMA and CS polymers, we expect these materials to be useful in the pharmaceutical industry as novel biomaterial composites with potential applications in drug delivery.
24183808	7	22	attach	presence	782:789	arg1	surface					810:816	the surface	806:816	the surface of the CS functionalized carbon nanotubes	806:858	Moreover, the presence of polyHEMA on the surface of the CS functionalized carbon nanotubes was confirmed by field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and thermo gravimetric analysis (TGA) analyses.
24183808	7	22	attach	presence	782:789	arg2	polyHEMA					794:801	polyHEMA	794:801	polyHEMA	794:801	Moreover, the presence of polyHEMA on the surface of the CS functionalized carbon nanotubes was confirmed by field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and thermo gravimetric analysis (TGA) analyses.
24183808	9	23	theme	novel	1276:1280	arg1	composites					1294:1303	novel biomaterial composites	1276:1303	novel biomaterial composites with potential applications in drug delivery	1276:1348	Considering the biomedical importance of polyHEMA and CS polymers, we expect these materials to be useful in the pharmaceutical industry as novel biomaterial composites with potential applications in drug delivery.
24183808	7	24	theme	field	877:881	arg1	FESEM					922:926	FESEM	922:926	FESEM	922:926	Moreover, the presence of polyHEMA on the surface of the CS functionalized carbon nanotubes was confirmed by field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and thermo gravimetric analysis (TGA) analyses.
24183808	7	24	theme	field	877:881	arg1	microscopy					910:919	field emission scanning electron microscopy	877:919	field emission scanning electron microscopy (FESEM)	877:927	Moreover, the presence of polyHEMA on the surface of the CS functionalized carbon nanotubes was confirmed by field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and thermo gravimetric analysis (TGA) analyses.
24183808	3	25	theme	carbon	525:530	arg1	nanotubes					532:540	the carbon nanotubes	521:540	the carbon nanotubes	521:540	First, using microwave irradiation, CS was grafted onto the surface and sidewall of the carbon nanotubes.
24183808	1	26	theme	chitosan	146:153	arg1	composites					212:221	chitosan (CS) functionalized multi-walled carbon nanotube (MWCNT) composites	146:221	chitosan (CS) functionalized multi-walled carbon nanotube (MWCNT) composites with a biomedically important polymer, poly-2-hydroxyethyl methacrylate (polyHEMA),	146:305	We report a simple method for engineering chitosan (CS) functionalized multi-walled carbon nanotube (MWCNT) composites with a biomedically important polymer, poly-2-hydroxyethyl methacrylate (polyHEMA), by chemical grafting HEMA monomers via free radical polymerization.
24183808	2	27	theme	polyHEMA	403:410	arg1	Functionalization					375:391	Functionalization	375:391	Functionalization of CS and polyHEMA	375:410	Functionalization of CS and polyHEMA occurred in three steps.
24183808	9	28	theme	biomaterial	1282:1292	arg1	composites					1294:1303	novel biomaterial composites	1276:1303	novel biomaterial composites with potential applications in drug delivery	1276:1348	Considering the biomedical importance of polyHEMA and CS polymers, we expect these materials to be useful in the pharmaceutical industry as novel biomaterial composites with potential applications in drug delivery.
24183808	0	29	theme	"	93:93	arg1	methods					95:101	" methods	93:101	" methods	93:101	Grafting chitosan and polyHEMA on carbon nanotubes surfaces: "grafting to" and "grafting from" methods.
24183808	6	30	theme	infrared	738:745	arg1	spectroscopy					754:765	infrared (FTIR) spectroscopy	738:765	infrared (FTIR) spectroscopy	738:765	Composite synthesis was confirmed by Fourier transform infrared (FTIR) spectroscopy.
24183808	7	31	theme	polyHEMA	794:801	arg1	presence					782:789	the presence	778:789	the presence of polyHEMA on the surface of the CS functionalized carbon nanotubes	778:858	Moreover, the presence of polyHEMA on the surface of the CS functionalized carbon nanotubes was confirmed by field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and thermo gravimetric analysis (TGA) analyses.
24183808	2	32	theme	CS	396:397	arg1	Functionalization					375:391	Functionalization	375:391	Functionalization of CS and polyHEMA	375:410	Functionalization of CS and polyHEMA occurred in three steps.
24183808	1	33	theme	important	243:251	arg1	polymer					253:259	a biomedically important polymer	228:259	a biomedically important polymer	228:259	We report a simple method for engineering chitosan (CS) functionalized multi-walled carbon nanotube (MWCNT) composites with a biomedically important polymer, poly-2-hydroxyethyl methacrylate (polyHEMA), by chemical grafting HEMA monomers via free radical polymerization.
24183808	1	33	theme	important	243:251	arg1	methacrylate					282:293	poly-2-hydroxyethyl methacrylate	262:293	poly-2-hydroxyethyl methacrylate (polyHEMA)	262:304	We report a simple method for engineering chitosan (CS) functionalized multi-walled carbon nanotube (MWCNT) composites with a biomedically important polymer, poly-2-hydroxyethyl methacrylate (polyHEMA), by chemical grafting HEMA monomers via free radical polymerization.
24183808	7	34	theme	gravimetric	980:990	arg1	analysis					992:999	thermo gravimetric analysis	973:999	thermo gravimetric analysis (TGA)	973:1005	Moreover, the presence of polyHEMA on the surface of the CS functionalized carbon nanotubes was confirmed by field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and thermo gravimetric analysis (TGA) analyses.
24183808	7	34	theme	gravimetric	980:990	arg1	TGA					1002:1004	TGA	1002:1004	TGA	1002:1004	Moreover, the presence of polyHEMA on the surface of the CS functionalized carbon nanotubes was confirmed by field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and thermo gravimetric analysis (TGA) analyses.
24183808	0	35	theme	carbon	34:39	arg1	surfaces					51:58	carbon nanotubes surfaces	34:58	carbon nanotubes surfaces: "grafting to" and "grafting from" methods	34:101	Grafting chitosan and polyHEMA on carbon nanotubes surfaces: "grafting to" and "grafting from" methods.
24183808	5	36	theme	HEMA	668:671	arg1	monomers					673:680	HEMA monomers	668:680	HEMA monomers	668:680	The final step involved free radical polymerization of HEMA monomers.
24183808	1	37	theme	free	346:349	arg1	polymerization					359:372	free radical polymerization	346:372	free radical polymerization	346:372	We report a simple method for engineering chitosan (CS) functionalized multi-walled carbon nanotube (MWCNT) composites with a biomedically important polymer, poly-2-hydroxyethyl methacrylate (polyHEMA), by chemical grafting HEMA monomers via free radical polymerization.
24183808	0	38	dep	surfaces	51:58	arg1	grafting					62:69	grafting	62:69	grafting	62:69	Grafting chitosan and polyHEMA on carbon nanotubes surfaces: "grafting to" and "grafting from" methods.
24183808	0	38	dep	surfaces	51:58	arg1	grafting					80:87	grafting	80:87	grafting	80:87	Grafting chitosan and polyHEMA on carbon nanotubes surfaces: "grafting to" and "grafting from" methods.
24183808	9	39	theme	drug	1336:1339	arg1	delivery					1341:1348	drug delivery	1336:1348	drug delivery	1336:1348	Considering the biomedical importance of polyHEMA and CS polymers, we expect these materials to be useful in the pharmaceutical industry as novel biomaterial composites with potential applications in drug delivery.
24183808	4	40	theme	matrix	597:602	arg1	surface					604:610	the polymeric matrix surface	583:610	the polymeric matrix surface	583:610	Second, HEMA monomers were grafted onto the polymeric matrix surface.
24183808	9	41	theme	potential	1310:1318	arg1	applications					1320:1331	potential applications	1310:1331	potential applications in drug delivery	1310:1348	Considering the biomedical importance of polyHEMA and CS polymers, we expect these materials to be useful in the pharmaceutical industry as novel biomaterial composites with potential applications in drug delivery.
24183808	5	42	theme	monomers	673:680	arg1	polymerization					650:663	free radical polymerization	637:663	free radical polymerization of HEMA monomers	637:680	The final step involved free radical polymerization of HEMA monomers.
24183808	1	43	theme	radical	351:357	arg1	polymerization					359:372	free radical polymerization	346:372	free radical polymerization	346:372	We report a simple method for engineering chitosan (CS) functionalized multi-walled carbon nanotube (MWCNT) composites with a biomedically important polymer, poly-2-hydroxyethyl methacrylate (polyHEMA), by chemical grafting HEMA monomers via free radical polymerization.
24183808	7	44	from	presence	782:789	arg1	surface					810:816	the surface	806:816	the surface of the CS functionalized carbon nanotubes	806:858	Moreover, the presence of polyHEMA on the surface of the CS functionalized carbon nanotubes was confirmed by field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and thermo gravimetric analysis (TGA) analyses.
24183808	1	45	with	composites	212:221	arg1	polymer					253:259	a biomedically important polymer	228:259	a biomedically important polymer	228:259	We report a simple method for engineering chitosan (CS) functionalized multi-walled carbon nanotube (MWCNT) composites with a biomedically important polymer, poly-2-hydroxyethyl methacrylate (polyHEMA), by chemical grafting HEMA monomers via free radical polymerization.
24183808	1	45	with	composites	212:221	arg1	methacrylate					282:293	poly-2-hydroxyethyl methacrylate	262:293	poly-2-hydroxyethyl methacrylate (polyHEMA)	262:304	We report a simple method for engineering chitosan (CS) functionalized multi-walled carbon nanotube (MWCNT) composites with a biomedically important polymer, poly-2-hydroxyethyl methacrylate (polyHEMA), by chemical grafting HEMA monomers via free radical polymerization.
24183808	1	46	theme	functionalized	160:173	arg1	composites					212:221	chitosan (CS) functionalized multi-walled carbon nanotube (MWCNT) composites	146:221	chitosan (CS) functionalized multi-walled carbon nanotube (MWCNT) composites with a biomedically important polymer, poly-2-hydroxyethyl methacrylate (polyHEMA),	146:305	We report a simple method for engineering chitosan (CS) functionalized multi-walled carbon nanotube (MWCNT) composites with a biomedically important polymer, poly-2-hydroxyethyl methacrylate (polyHEMA), by chemical grafting HEMA monomers via free radical polymerization.
24183808	1	47	theme	poly-2-hydroxyethyl	262:280	arg1	polyHEMA					296:303	polyHEMA	296:303	polyHEMA	296:303	We report a simple method for engineering chitosan (CS) functionalized multi-walled carbon nanotube (MWCNT) composites with a biomedically important polymer, poly-2-hydroxyethyl methacrylate (polyHEMA), by chemical grafting HEMA monomers via free radical polymerization.
24183808	1	47	theme	poly-2-hydroxyethyl	262:280	arg1	polymer					253:259	a biomedically important polymer	228:259	a biomedically important polymer	228:259	We report a simple method for engineering chitosan (CS) functionalized multi-walled carbon nanotube (MWCNT) composites with a biomedically important polymer, poly-2-hydroxyethyl methacrylate (polyHEMA), by chemical grafting HEMA monomers via free radical polymerization.
24183808	1	47	theme	poly-2-hydroxyethyl	262:280	arg1	methacrylate					282:293	poly-2-hydroxyethyl methacrylate	262:293	poly-2-hydroxyethyl methacrylate (polyHEMA)	262:304	We report a simple method for engineering chitosan (CS) functionalized multi-walled carbon nanotube (MWCNT) composites with a biomedically important polymer, poly-2-hydroxyethyl methacrylate (polyHEMA), by chemical grafting HEMA monomers via free radical polymerization.
24183808	3	48	theme	nanotubes	532:540	arg1	surface					497:503	surface	497:503	surface	497:503	First, using microwave irradiation, CS was grafted onto the surface and sidewall of the carbon nanotubes.
24183808	3	48	theme	nanotubes	532:540	arg1	sidewall					509:516	sidewall	509:516	sidewall	509:516	First, using microwave irradiation, CS was grafted onto the surface and sidewall of the carbon nanotubes.
24183808	7	49	theme	thermo	973:978	arg1	analysis					992:999	thermo gravimetric analysis	973:999	thermo gravimetric analysis (TGA)	973:1005	Moreover, the presence of polyHEMA on the surface of the CS functionalized carbon nanotubes was confirmed by field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and thermo gravimetric analysis (TGA) analyses.
24183808	7	49	theme	thermo	973:978	arg1	TGA					1002:1004	TGA	1002:1004	TGA	1002:1004	Moreover, the presence of polyHEMA on the surface of the CS functionalized carbon nanotubes was confirmed by field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and thermo gravimetric analysis (TGA) analyses.
24183808	3	50	theme	microwave	450:458	arg1	irradiation					460:470	microwave irradiation	450:470	microwave irradiation	450:470	First, using microwave irradiation, CS was grafted onto the surface and sidewall of the carbon nanotubes.
24183808	0	51	theme	nanotubes	41:49	arg1	surfaces					51:58	carbon nanotubes surfaces	34:58	carbon nanotubes surfaces: "grafting to" and "grafting from" methods	34:101	Grafting chitosan and polyHEMA on carbon nanotubes surfaces: "grafting to" and "grafting from" methods.
24183808	8	52	theme	aqueous	1037:1043	arg1	phase					1045:1049	the aqueous phase	1033:1049	the aqueous phase	1033:1049	Furthermore, in the aqueous phase, our novel composites exhibited higher dispersibility compared with pristine MWCNTs.
24183808	9	53	theme	polymers	1193:1200	arg1	importance					1163:1172	the biomedical importance	1148:1172	the biomedical importance of polyHEMA and CS polymers	1148:1200	Considering the biomedical importance of polyHEMA and CS polymers, we expect these materials to be useful in the pharmaceutical industry as novel biomaterial composites with potential applications in drug delivery.
24183808	7	54	dep	microscopy	910:919	arg1	analyses					1007:1014	analyses	1007:1014	analyses	1007:1014	Moreover, the presence of polyHEMA on the surface of the CS functionalized carbon nanotubes was confirmed by field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and thermo gravimetric analysis (TGA) analyses.
24183808	7	55	theme	emission	883:890	arg1	FESEM					922:926	FESEM	922:926	FESEM	922:926	Moreover, the presence of polyHEMA on the surface of the CS functionalized carbon nanotubes was confirmed by field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and thermo gravimetric analysis (TGA) analyses.
24183808	7	55	theme	emission	883:890	arg1	microscopy					910:919	field emission scanning electron microscopy	877:919	field emission scanning electron microscopy (FESEM)	877:927	Moreover, the presence of polyHEMA on the surface of the CS functionalized carbon nanotubes was confirmed by field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and thermo gravimetric analysis (TGA) analyses.
24183808	5	56	theme	free	637:640	arg1	polymerization					650:663	free radical polymerization	637:663	free radical polymerization of HEMA monomers	637:680	The final step involved free radical polymerization of HEMA monomers.
24183808	9	57	theme	biomedical	1152:1161	arg1	importance					1163:1172	the biomedical importance	1148:1172	the biomedical importance of polyHEMA and CS polymers	1148:1200	Considering the biomedical importance of polyHEMA and CS polymers, we expect these materials to be useful in the pharmaceutical industry as novel biomaterial composites with potential applications in drug delivery.
24183808	3	58	dep	surface	497:503	arg1	the					493:495	the	493:495	the	493:495	First, using microwave irradiation, CS was grafted onto the surface and sidewall of the carbon nanotubes.
24183808	6	59	theme	Composite	683:691	arg1	synthesis					693:701	Composite synthesis	683:701	Composite synthesis	683:701	Composite synthesis was confirmed by Fourier transform infrared (FTIR) spectroscopy.
24183808	1	60	theme	multi-walled	175:186	arg1	composites					212:221	chitosan (CS) functionalized multi-walled carbon nanotube (MWCNT) composites	146:221	chitosan (CS) functionalized multi-walled carbon nanotube (MWCNT) composites with a biomedically important polymer, poly-2-hydroxyethyl methacrylate (polyHEMA),	146:305	We report a simple method for engineering chitosan (CS) functionalized multi-walled carbon nanotube (MWCNT) composites with a biomedically important polymer, poly-2-hydroxyethyl methacrylate (polyHEMA), by chemical grafting HEMA monomers via free radical polymerization.
24183808	5	61	theme	radical	642:648	arg1	polymerization					650:663	free radical polymerization	637:663	free radical polymerization of HEMA monomers	637:680	The final step involved free radical polymerization of HEMA monomers.
24183808	7	62	theme	electron	943:950	arg1	TEM					964:966	TEM	964:966	TEM	964:966	Moreover, the presence of polyHEMA on the surface of the CS functionalized carbon nanotubes was confirmed by field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and thermo gravimetric analysis (TGA) analyses.
24183808	7	62	theme	electron	943:950	arg1	microscopy					952:961	transmission electron microscopy	930:961	transmission electron microscopy (TEM)	930:967	Moreover, the presence of polyHEMA on the surface of the CS functionalized carbon nanotubes was confirmed by field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and thermo gravimetric analysis (TGA) analyses.
24183808	7	63	theme	CS	825:826	arg1	nanotubes					850:858	the CS functionalized carbon nanotubes	821:858	the CS functionalized carbon nanotubes	821:858	Moreover, the presence of polyHEMA on the surface of the CS functionalized carbon nanotubes was confirmed by field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and thermo gravimetric analysis (TGA) analyses.
24183808	7	64	theme	nanotubes	850:858	arg1	surface					810:816	the surface	806:816	the surface of the CS functionalized carbon nanotubes	806:858	Moreover, the presence of polyHEMA on the surface of the CS functionalized carbon nanotubes was confirmed by field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and thermo gravimetric analysis (TGA) analyses.
24183808	1	65	theme	carbon	188:193	arg1	composites					212:221	chitosan (CS) functionalized multi-walled carbon nanotube (MWCNT) composites	146:221	chitosan (CS) functionalized multi-walled carbon nanotube (MWCNT) composites with a biomedically important polymer, poly-2-hydroxyethyl methacrylate (polyHEMA),	146:305	We report a simple method for engineering chitosan (CS) functionalized multi-walled carbon nanotube (MWCNT) composites with a biomedically important polymer, poly-2-hydroxyethyl methacrylate (polyHEMA), by chemical grafting HEMA monomers via free radical polymerization.
24183808	4	66	theme	polymeric	587:595	arg1	surface					604:610	the polymeric matrix surface	583:610	the polymeric matrix surface	583:610	Second, HEMA monomers were grafted onto the polymeric matrix surface.
24183808	1	67	theme	nanotube	195:202	arg1	composites					212:221	chitosan (CS) functionalized multi-walled carbon nanotube (MWCNT) composites	146:221	chitosan (CS) functionalized multi-walled carbon nanotube (MWCNT) composites with a biomedically important polymer, poly-2-hydroxyethyl methacrylate (polyHEMA),	146:305	We report a simple method for engineering chitosan (CS) functionalized multi-walled carbon nanotube (MWCNT) composites with a biomedically important polymer, poly-2-hydroxyethyl methacrylate (polyHEMA), by chemical grafting HEMA monomers via free radical polymerization.
24183808	8	68	theme	novel	1056:1060	arg1	composites					1062:1071	our novel composites	1052:1071	our novel composites	1052:1071	Furthermore, in the aqueous phase, our novel composites exhibited higher dispersibility compared with pristine MWCNTs.
24183808	4	69	theme	HEMA	551:554	arg1	monomers					556:563	HEMA monomers	551:563	HEMA monomers	551:563	Second, HEMA monomers were grafted onto the polymeric matrix surface.
24183808	7	70	theme	functionalized	828:841	arg1	nanotubes					850:858	the CS functionalized carbon nanotubes	821:858	the CS functionalized carbon nanotubes	821:858	Moreover, the presence of polyHEMA on the surface of the CS functionalized carbon nanotubes was confirmed by field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and thermo gravimetric analysis (TGA) analyses.
28953376	2	0	theme	bioinformatics	575:588	arg1	analysis					590:597	structural bioinformatics analysis	564:597	structural bioinformatics analysis	564:597	To further elucidate the effects of N-glycosylation on protein structure and to provide principles useful for the rational design of α-helical glycopeptides, we investigate stabilizing protein-sugar interactions in α-helical glycosylation sites using an integrated structural bioinformatics analysis and molecular dynamics simulation approach.
28953376	2	1	from	interactions	498:509	arg1	sites					538:542	α-helical glycosylation sites	514:542	α-helical glycosylation sites using an integrated structural bioinformatics analysis and molecular dynamics simulation approach	514:640	To further elucidate the effects of N-glycosylation on protein structure and to provide principles useful for the rational design of α-helical glycopeptides, we investigate stabilizing protein-sugar interactions in α-helical glycosylation sites using an integrated structural bioinformatics analysis and molecular dynamics simulation approach.
28953376	1	2	theme	glycoprotein	170:181	arg1	stability					183:191	glycoprotein stability	170:191	glycoprotein stability	170:191	Glycosylation not only impacts the functions of glycoproteins but can also improve glycoprotein stability and folding efficiency-characteristics that are desirable for protein engineering and therapeutic design.
28953376	4	3	theme	enthalpy	876:883	arg1	combination					821:831	A combination	819:831	A combination of sterics and favorable intraglycopeptide enthalpy	819:883	A combination of sterics and favorable intraglycopeptide enthalpy explains the existence of only these two conformations.
28953376	2	4	theme	structural	564:573	arg1	analysis					590:597	structural bioinformatics analysis	564:597	structural bioinformatics analysis	564:597	To further elucidate the effects of N-glycosylation on protein structure and to provide principles useful for the rational design of α-helical glycopeptides, we investigate stabilizing protein-sugar interactions in α-helical glycosylation sites using an integrated structural bioinformatics analysis and molecular dynamics simulation approach.
28953376	6	5	theme	=	1165:1165	arg1	300°					1167:1170	χ1 = 300°	1162:1170	χ1 = 300°	1162:1170	The most common interactions involve either a Glu residue at the -4 position interacting with the GlcNAc whose Asn has χ1 = 300° or a Glu residue at the +4 position interacting with the GlcNAc whose Asn has χ1 = 180°.
28953376	1	6	theme	stability	183:191	arg1	efficiency-characteristics					205:230	glycoprotein stability and folding efficiency-characteristics	170:230	glycoprotein stability and folding efficiency-characteristics that are desirable for protein engineering and therapeutic design	170:296	Glycosylation not only impacts the functions of glycoproteins but can also improve glycoprotein stability and folding efficiency-characteristics that are desirable for protein engineering and therapeutic design.
28953376	7	7	theme	intraglycopeptide	1446:1462	arg1	interactions					1464:1475	favorable electrostatic intraglycopeptide interactions	1422:1475	favorable electrostatic intraglycopeptide interactions	1422:1475	Via metadynamics simulations of model α-helical glycopeptides with each of these two interactions, we find that both interactions are stabilizing as a result of favorable electrostatic intraglycopeptide interactions.
28953376	7	8	theme	interactions	1464:1475	arg1	result					1412:1417	a result	1410:1417	a result of favorable electrostatic intraglycopeptide interactions	1410:1475	Via metadynamics simulations of model α-helical glycopeptides with each of these two interactions, we find that both interactions are stabilizing as a result of favorable electrostatic intraglycopeptide interactions.
28953376	2	9	theme	N-glycosylation	335:349	arg1	effects					324:330	the effects	320:330	the effects of N-glycosylation on protein structure	320:370	To further elucidate the effects of N-glycosylation on protein structure and to provide principles useful for the rational design of α-helical glycopeptides, we investigate stabilizing protein-sugar interactions in α-helical glycosylation sites using an integrated structural bioinformatics analysis and molecular dynamics simulation approach.
28953376	2	10	theme	simulation	622:631	arg1	approach					633:640	an integrated structural bioinformatics analysis and molecular dynamics simulation approach	550:640	an integrated structural bioinformatics analysis and molecular dynamics simulation approach	550:640	To further elucidate the effects of N-glycosylation on protein structure and to provide principles useful for the rational design of α-helical glycopeptides, we investigate stabilizing protein-sugar interactions in α-helical glycosylation sites using an integrated structural bioinformatics analysis and molecular dynamics simulation approach.
28953376	8	11	theme	useful	1592:1597	arg1	strategy					1599:1606	a useful strategy	1590:1606	a useful strategy for engineering stable α-helical glycoproteins	1590:1653	Thus, we suggest that incorporating a Glu at either the -4 or +4 position relative to an N-linked glycan may be a useful strategy for engineering stable α-helical glycoproteins.
28953376	4	12	theme	favorable	848:856	arg1	enthalpy					876:883	favorable intraglycopeptide enthalpy	848:883	favorable intraglycopeptide enthalpy	848:883	A combination of sterics and favorable intraglycopeptide enthalpy explains the existence of only these two conformations.
28953376	6	13	contain	has	1246:1248	arg1	Asn					1242:1244	Asn	1242:1244	Asn	1242:1244	The most common interactions involve either a Glu residue at the -4 position interacting with the GlcNAc whose Asn has χ1 = 300° or a Glu residue at the +4 position interacting with the GlcNAc whose Asn has χ1 = 180°.
28953376	6	13	contain	has	1246:1248	arg2	χ1					1250:1251	χ1	1250:1251	χ1 = 180°	1250:1258	The most common interactions involve either a Glu residue at the -4 position interacting with the GlcNAc whose Asn has χ1 = 300° or a Glu residue at the +4 position interacting with the GlcNAc whose Asn has χ1 = 180°.
28953376	2	14	theme	dynamics	613:620	arg1	approach					633:640	an integrated structural bioinformatics analysis and molecular dynamics simulation approach	550:640	an integrated structural bioinformatics analysis and molecular dynamics simulation approach	550:640	To further elucidate the effects of N-glycosylation on protein structure and to provide principles useful for the rational design of α-helical glycopeptides, we investigate stabilizing protein-sugar interactions in α-helical glycosylation sites using an integrated structural bioinformatics analysis and molecular dynamics simulation approach.
28953376	6	15	contain	has	1158:1160	arg1	Asn					1154:1156	Asn	1154:1156	Asn	1154:1156	The most common interactions involve either a Glu residue at the -4 position interacting with the GlcNAc whose Asn has χ1 = 300° or a Glu residue at the +4 position interacting with the GlcNAc whose Asn has χ1 = 180°.
28953376	6	15	contain	has	1158:1160	arg2	300°					1167:1170	χ1 = 300°	1162:1170	χ1 = 300°	1162:1170	The most common interactions involve either a Glu residue at the -4 position interacting with the GlcNAc whose Asn has χ1 = 300° or a Glu residue at the +4 position interacting with the GlcNAc whose Asn has χ1 = 180°.
28953376	2	16	gly	glycopeptides	442:454	arg2	glycopeptides					442:454	α-helical glycopeptides	432:454	α-helical glycopeptides	432:454	To further elucidate the effects of N-glycosylation on protein structure and to provide principles useful for the rational design of α-helical glycopeptides, we investigate stabilizing protein-sugar interactions in α-helical glycosylation sites using an integrated structural bioinformatics analysis and molecular dynamics simulation approach.
28953376	6	17	theme	=	1253:1253	arg1	χ1					1250:1251	χ1	1250:1251	χ1 = 180°	1250:1258	The most common interactions involve either a Glu residue at the -4 position interacting with the GlcNAc whose Asn has χ1 = 300° or a Glu residue at the +4 position interacting with the GlcNAc whose Asn has χ1 = 180°.
28953376	5	18	theme	protein-sugar	975:987	arg1	interactions					989:1000	all known protein-sugar interactions	965:1000	all known protein-sugar interactions that utilize these conformational modes	965:1040	Furthermore, we catalog all known protein-sugar interactions that utilize these conformational modes.
28953376	6	19	theme	Glu	1177:1179	arg1	residue					1181:1187	a Glu residue	1175:1187	a Glu residue at the +4 position interacting with the GlcNAc whose Asn has χ1 = 180°	1175:1258	The most common interactions involve either a Glu residue at the -4 position interacting with the GlcNAc whose Asn has χ1 = 300° or a Glu residue at the +4 position interacting with the GlcNAc whose Asn has χ1 = 180°.
28953376	2	20	theme	molecular	603:611	arg1	dynamics					613:620	molecular dynamics	603:620	molecular dynamics	603:620	To further elucidate the effects of N-glycosylation on protein structure and to provide principles useful for the rational design of α-helical glycopeptides, we investigate stabilizing protein-sugar interactions in α-helical glycosylation sites using an integrated structural bioinformatics analysis and molecular dynamics simulation approach.
28953376	3	21	theme	Data	808:811	arg1	Bank					813:816	the Protein Data Bank	796:816	the Protein Data Bank	796:816	We identify two glycan conformations with an Asn χ1 of 180° or 300° that are amenable to α-helical structure in natural α-helical glycosylation sites in the Protein Data Bank.
28953376	3	22	from	sites	787:791	arg1	Bank					813:816	the Protein Data Bank	796:816	the Protein Data Bank	796:816	We identify two glycan conformations with an Asn χ1 of 180° or 300° that are amenable to α-helical structure in natural α-helical glycosylation sites in the Protein Data Bank.
28953376	6	23	dep	=	1253:1253	arg1	180°					1255:1258	180°	1255:1258	180°	1255:1258	The most common interactions involve either a Glu residue at the -4 position interacting with the GlcNAc whose Asn has χ1 = 300° or a Glu residue at the +4 position interacting with the GlcNAc whose Asn has χ1 = 180°.
28953376	3	24	with	conformations	666:678	arg1	χ1					692:693	an Asn χ1	685:693	an Asn χ1 of 180° or 300°	685:709	We identify two glycan conformations with an Asn χ1 of 180° or 300° that are amenable to α-helical structure in natural α-helical glycosylation sites in the Protein Data Bank.
28953376	1	25	theme	folding	197:203	arg1	efficiency-characteristics					205:230	glycoprotein stability and folding efficiency-characteristics	170:230	glycoprotein stability and folding efficiency-characteristics that are desirable for protein engineering and therapeutic design	170:296	Glycosylation not only impacts the functions of glycoproteins but can also improve glycoprotein stability and folding efficiency-characteristics that are desirable for protein engineering and therapeutic design.
28953376	2	26	theme	glycosylation	524:536	arg1	sites					538:542	α-helical glycosylation sites	514:542	α-helical glycosylation sites using an integrated structural bioinformatics analysis and molecular dynamics simulation approach	514:640	To further elucidate the effects of N-glycosylation on protein structure and to provide principles useful for the rational design of α-helical glycopeptides, we investigate stabilizing protein-sugar interactions in α-helical glycosylation sites using an integrated structural bioinformatics analysis and molecular dynamics simulation approach.
28953376	7	27	theme	α-helical	1299:1307	arg1	glycopeptides					1309:1321	model α-helical glycopeptides	1293:1321	model α-helical glycopeptides with each of these two interactions	1293:1357	Via metadynamics simulations of model α-helical glycopeptides with each of these two interactions, we find that both interactions are stabilizing as a result of favorable electrostatic intraglycopeptide interactions.
28953376	7	28	gly	intraglycopeptide	1446:1462	arg2	intraglycopeptide					1446:1462	favorable electrostatic intraglycopeptide interactions	1422:1475	favorable electrostatic intraglycopeptide interactions	1422:1475	Via metadynamics simulations of model α-helical glycopeptides with each of these two interactions, we find that both interactions are stabilizing as a result of favorable electrostatic intraglycopeptide interactions.
28953376	2	29	theme	α-helical	432:440	arg1	glycopeptides					442:454	α-helical glycopeptides	432:454	α-helical glycopeptides	432:454	To further elucidate the effects of N-glycosylation on protein structure and to provide principles useful for the rational design of α-helical glycopeptides, we investigate stabilizing protein-sugar interactions in α-helical glycosylation sites using an integrated structural bioinformatics analysis and molecular dynamics simulation approach.
28953376	2	30	theme	α-helical	514:522	arg1	sites					538:542	α-helical glycosylation sites	514:542	α-helical glycosylation sites using an integrated structural bioinformatics analysis and molecular dynamics simulation approach	514:640	To further elucidate the effects of N-glycosylation on protein structure and to provide principles useful for the rational design of α-helical glycopeptides, we investigate stabilizing protein-sugar interactions in α-helical glycosylation sites using an integrated structural bioinformatics analysis and molecular dynamics simulation approach.
28953376	8	31	theme	stable	1624:1629	arg1	glycoproteins					1641:1653	stable α-helical glycoproteins	1624:1653	stable α-helical glycoproteins	1624:1653	Thus, we suggest that incorporating a Glu at either the -4 or +4 position relative to an N-linked glycan may be a useful strategy for engineering stable α-helical glycoproteins.
28953376	6	32	from	position	1111:1118	arg1	residue					1181:1187	a Glu residue	1175:1187	a Glu residue at the +4 position interacting with the GlcNAc whose Asn has χ1 = 180°	1175:1258	The most common interactions involve either a Glu residue at the -4 position interacting with the GlcNAc whose Asn has χ1 = 300° or a Glu residue at the +4 position interacting with the GlcNAc whose Asn has χ1 = 180°.
28953376	6	32	from	position	1111:1118	arg1	residue					1093:1099	a Glu residue	1087:1099	a Glu residue at the -4 position interacting with the GlcNAc whose Asn has χ1 = 300°	1087:1170	The most common interactions involve either a Glu residue at the -4 position interacting with the GlcNAc whose Asn has χ1 = 300° or a Glu residue at the +4 position interacting with the GlcNAc whose Asn has χ1 = 180°.
28953376	8	33	gly	glycoproteins	1641:1653	arg1	glycoproteins					1641:1653	stable α-helical glycoproteins	1624:1653	stable α-helical glycoproteins	1624:1653	Thus, we suggest that incorporating a Glu at either the -4 or +4 position relative to an N-linked glycan may be a useful strategy for engineering stable α-helical glycoproteins.
28953376	5	34	theme	known	969:973	arg1	interactions					989:1000	all known protein-sugar interactions	965:1000	all known protein-sugar interactions that utilize these conformational modes	965:1040	Furthermore, we catalog all known protein-sugar interactions that utilize these conformational modes.
28953376	4	35	theme	intraglycopeptide	858:874	arg1	enthalpy					876:883	favorable intraglycopeptide enthalpy	848:883	favorable intraglycopeptide enthalpy	848:883	A combination of sterics and favorable intraglycopeptide enthalpy explains the existence of only these two conformations.
28953376	5	36	theme	conformational	1021:1034	arg1	modes					1036:1040	these conformational modes	1015:1040	these conformational modes	1015:1040	Furthermore, we catalog all known protein-sugar interactions that utilize these conformational modes.
28953376	0	37	theme	Glycopeptide	26:37	arg1	Design					39:44	α-Helical Glycopeptide Design	16:44	α-Helical Glycopeptide Design	16:44	Predictions for α-Helical Glycopeptide Design from Structural Bioinformatics Analysis.
28953376	1	38	gly	glycoproteins	135:147	arg1	glycoproteins					135:147	glycoproteins	135:147	glycoproteins	135:147	Glycosylation not only impacts the functions of glycoproteins but can also improve glycoprotein stability and folding efficiency-characteristics that are desirable for protein engineering and therapeutic design.
28953376	7	39	theme	favorable	1422:1430	arg1	interactions					1464:1475	favorable electrostatic intraglycopeptide interactions	1422:1475	favorable electrostatic intraglycopeptide interactions	1422:1475	Via metadynamics simulations of model α-helical glycopeptides with each of these two interactions, we find that both interactions are stabilizing as a result of favorable electrostatic intraglycopeptide interactions.
28953376	2	40	theme	protein	354:360	arg1	structure					362:370	protein structure	354:370	protein structure	354:370	To further elucidate the effects of N-glycosylation on protein structure and to provide principles useful for the rational design of α-helical glycopeptides, we investigate stabilizing protein-sugar interactions in α-helical glycosylation sites using an integrated structural bioinformatics analysis and molecular dynamics simulation approach.
28953376	2	41	theme	protein-sugar	484:496	arg1	interactions					498:509	stabilizing protein-sugar interactions	472:509	stabilizing protein-sugar interactions in α-helical glycosylation sites using an integrated structural bioinformatics analysis and molecular dynamics simulation approach	472:640	To further elucidate the effects of N-glycosylation on protein structure and to provide principles useful for the rational design of α-helical glycopeptides, we investigate stabilizing protein-sugar interactions in α-helical glycosylation sites using an integrated structural bioinformatics analysis and molecular dynamics simulation approach.
28953376	0	42	theme	α-Helical	16:24	arg1	Design					39:44	α-Helical Glycopeptide Design	16:44	α-Helical Glycopeptide Design	16:44	Predictions for α-Helical Glycopeptide Design from Structural Bioinformatics Analysis.
28953376	6	43	theme	Glu	1089:1091	arg1	residue					1093:1099	a Glu residue	1087:1099	a Glu residue at the -4 position interacting with the GlcNAc whose Asn has χ1 = 300°	1087:1170	The most common interactions involve either a Glu residue at the -4 position interacting with the GlcNAc whose Asn has χ1 = 300° or a Glu residue at the +4 position interacting with the GlcNAc whose Asn has χ1 = 180°.
28953376	3	44	theme	α-helical	732:740	arg1	structure					742:750	α-helical structure	732:750	α-helical structure in natural α-helical glycosylation sites in the Protein Data Bank	732:816	We identify two glycan conformations with an Asn χ1 of 180° or 300° that are amenable to α-helical structure in natural α-helical glycosylation sites in the Protein Data Bank.
28953376	4	45	theme	sterics	836:842	arg1	combination					821:831	A combination	819:831	A combination of sterics and favorable intraglycopeptide enthalpy	819:883	A combination of sterics and favorable intraglycopeptide enthalpy explains the existence of only these two conformations.
28953376	2	46	theme	stabilizing	472:482	arg1	interactions					498:509	stabilizing protein-sugar interactions	472:509	stabilizing protein-sugar interactions in α-helical glycosylation sites using an integrated structural bioinformatics analysis and molecular dynamics simulation approach	472:640	To further elucidate the effects of N-glycosylation on protein structure and to provide principles useful for the rational design of α-helical glycopeptides, we investigate stabilizing protein-sugar interactions in α-helical glycosylation sites using an integrated structural bioinformatics analysis and molecular dynamics simulation approach.
28953376	2	47	theme	integrated	553:562	arg1	approach					633:640	an integrated structural bioinformatics analysis and molecular dynamics simulation approach	550:640	an integrated structural bioinformatics analysis and molecular dynamics simulation approach	550:640	To further elucidate the effects of N-glycosylation on protein structure and to provide principles useful for the rational design of α-helical glycopeptides, we investigate stabilizing protein-sugar interactions in α-helical glycosylation sites using an integrated structural bioinformatics analysis and molecular dynamics simulation approach.
28953376	8	48	theme	α-helical	1631:1639	arg1	glycoproteins					1641:1653	stable α-helical glycoproteins	1624:1653	stable α-helical glycoproteins	1624:1653	Thus, we suggest that incorporating a Glu at either the -4 or +4 position relative to an N-linked glycan may be a useful strategy for engineering stable α-helical glycoproteins.
28953376	7	49	theme	model	1293:1297	arg1	glycopeptides					1309:1321	model α-helical glycopeptides	1293:1321	model α-helical glycopeptides with each of these two interactions	1293:1357	Via metadynamics simulations of model α-helical glycopeptides with each of these two interactions, we find that both interactions are stabilizing as a result of favorable electrostatic intraglycopeptide interactions.
28953376	3	50	theme	180°	698:701	arg1	χ1					692:693	an Asn χ1	685:693	an Asn χ1 of 180° or 300°	685:709	We identify two glycan conformations with an Asn χ1 of 180° or 300° that are amenable to α-helical structure in natural α-helical glycosylation sites in the Protein Data Bank.
28953376	1	51	theme	protein	255:261	arg1	engineering					263:273	protein engineering	255:273	protein engineering	255:273	Glycosylation not only impacts the functions of glycoproteins but can also improve glycoprotein stability and folding efficiency-characteristics that are desirable for protein engineering and therapeutic design.
28953376	0	52	theme	Bioinformatics	62:75	arg1	Analysis					77:84	Structural Bioinformatics Analysis	51:84	Structural Bioinformatics Analysis	51:84	Predictions for α-Helical Glycopeptide Design from Structural Bioinformatics Analysis.
28953376	0	53	from	Analysis	77:84	arg1	Predictions					0:10	Predictions	0:10	Predictions for α-Helical Glycopeptide Design from Structural Bioinformatics Analysis	0:84	Predictions for α-Helical Glycopeptide Design from Structural Bioinformatics Analysis.
28953376	3	54	theme	Asn	688:690	arg1	χ1					692:693	an Asn χ1	685:693	an Asn χ1 of 180° or 300°	685:709	We identify two glycan conformations with an Asn χ1 of 180° or 300° that are amenable to α-helical structure in natural α-helical glycosylation sites in the Protein Data Bank.
28953376	6	55	from	position	1199:1206	arg1	residue					1181:1187	a Glu residue	1175:1187	a Glu residue at the +4 position interacting with the GlcNAc whose Asn has χ1 = 180°	1175:1258	The most common interactions involve either a Glu residue at the -4 position interacting with the GlcNAc whose Asn has χ1 = 300° or a Glu residue at the +4 position interacting with the GlcNAc whose Asn has χ1 = 180°.
28953376	6	55	from	position	1199:1206	arg1	residue					1093:1099	a Glu residue	1087:1099	a Glu residue at the -4 position interacting with the GlcNAc whose Asn has χ1 = 300°	1087:1170	The most common interactions involve either a Glu residue at the -4 position interacting with the GlcNAc whose Asn has χ1 = 300° or a Glu residue at the +4 position interacting with the GlcNAc whose Asn has χ1 = 180°.
28953376	2	56	theme	useful	398:403	arg1	principles					387:396	principles	387:396	principles useful for the rational design of α-helical glycopeptides	387:454	To further elucidate the effects of N-glycosylation on protein structure and to provide principles useful for the rational design of α-helical glycopeptides, we investigate stabilizing protein-sugar interactions in α-helical glycosylation sites using an integrated structural bioinformatics analysis and molecular dynamics simulation approach.
28953376	0	57	theme	Structural	51:60	arg1	Analysis					77:84	Structural Bioinformatics Analysis	51:84	Structural Bioinformatics Analysis	51:84	Predictions for α-Helical Glycopeptide Design from Structural Bioinformatics Analysis.
28953376	7	58	theme	metadynamics	1265:1276	arg1	simulations					1278:1288	metadynamics simulations	1265:1288	metadynamics simulations of model α-helical glycopeptides with each of these two interactions	1265:1357	Via metadynamics simulations of model α-helical glycopeptides with each of these two interactions, we find that both interactions are stabilizing as a result of favorable electrostatic intraglycopeptide interactions.
28953376	3	59	theme	α-helical	763:771	arg1	sites					787:791	natural α-helical glycosylation sites	755:791	natural α-helical glycosylation sites in the Protein Data Bank	755:816	We identify two glycan conformations with an Asn χ1 of 180° or 300° that are amenable to α-helical structure in natural α-helical glycosylation sites in the Protein Data Bank.
28953376	4	60	theme	conformations	926:938	arg1	existence					898:906	the existence	894:906	the existence of only these two conformations	894:938	A combination of sterics and favorable intraglycopeptide enthalpy explains the existence of only these two conformations.
28953376	7	61	gly	glycopeptides	1309:1321	arg2	glycopeptides					1309:1321	model α-helical glycopeptides	1293:1321	model α-helical glycopeptides with each of these two interactions	1293:1357	Via metadynamics simulations of model α-helical glycopeptides with each of these two interactions, we find that both interactions are stabilizing as a result of favorable electrostatic intraglycopeptide interactions.
28953376	1	62	theme	glycoproteins	135:147	arg1	functions					122:130	the functions	118:130	the functions of glycoproteins	118:147	Glycosylation not only impacts the functions of glycoproteins but can also improve glycoprotein stability and folding efficiency-characteristics that are desirable for protein engineering and therapeutic design.
28953376	2	63	from	effects	324:330	arg1	structure					362:370	protein structure	354:370	protein structure	354:370	To further elucidate the effects of N-glycosylation on protein structure and to provide principles useful for the rational design of α-helical glycopeptides, we investigate stabilizing protein-sugar interactions in α-helical glycosylation sites using an integrated structural bioinformatics analysis and molecular dynamics simulation approach.
28953376	6	64	theme	common	1052:1057	arg1	interactions					1059:1070	The most common interactions	1043:1070	The most common interactions	1043:1070	The most common interactions involve either a Glu residue at the -4 position interacting with the GlcNAc whose Asn has χ1 = 300° or a Glu residue at the +4 position interacting with the GlcNAc whose Asn has χ1 = 180°.
28953376	3	65	theme	glycosylation	773:785	arg1	sites					787:791	natural α-helical glycosylation sites	755:791	natural α-helical glycosylation sites in the Protein Data Bank	755:816	We identify two glycan conformations with an Asn χ1 of 180° or 300° that are amenable to α-helical structure in natural α-helical glycosylation sites in the Protein Data Bank.
28953376	7	66	theme	electrostatic	1432:1444	arg1	interactions					1464:1475	favorable electrostatic intraglycopeptide interactions	1422:1475	favorable electrostatic intraglycopeptide interactions	1422:1475	Via metadynamics simulations of model α-helical glycopeptides with each of these two interactions, we find that both interactions are stabilizing as a result of favorable electrostatic intraglycopeptide interactions.
28953376	3	67	theme	glycan	659:664	arg1	conformations					666:678	two glycan conformations	655:678	two glycan conformations with an Asn χ1 of 180° or 300° that are amenable to α-helical structure in natural α-helical glycosylation sites in the Protein Data Bank	655:816	We identify two glycan conformations with an Asn χ1 of 180° or 300° that are amenable to α-helical structure in natural α-helical glycosylation sites in the Protein Data Bank.
28953376	3	68	gly	glycosylation	773:785	arg2	sites					787:791	natural α-helical glycosylation sites	755:791	natural α-helical glycosylation sites in the Protein Data Bank	755:816	We identify two glycan conformations with an Asn χ1 of 180° or 300° that are amenable to α-helical structure in natural α-helical glycosylation sites in the Protein Data Bank.
28953376	1	69	gly	glycoprotein	170:181	arg1	glycoprotein					170:181	glycoprotein stability	170:191	glycoprotein stability	170:191	Glycosylation not only impacts the functions of glycoproteins but can also improve glycoprotein stability and folding efficiency-characteristics that are desirable for protein engineering and therapeutic design.
28953376	2	70	gly	glycosylation	524:536	arg2	sites					538:542	α-helical glycosylation sites	514:542	α-helical glycosylation sites using an integrated structural bioinformatics analysis and molecular dynamics simulation approach	514:640	To further elucidate the effects of N-glycosylation on protein structure and to provide principles useful for the rational design of α-helical glycopeptides, we investigate stabilizing protein-sugar interactions in α-helical glycosylation sites using an integrated structural bioinformatics analysis and molecular dynamics simulation approach.
28953376	8	71	theme	N-linked	1567:1574	arg1	glycan					1576:1581	an N-linked glycan	1564:1581	an N-linked glycan	1564:1581	Thus, we suggest that incorporating a Glu at either the -4 or +4 position relative to an N-linked glycan may be a useful strategy for engineering stable α-helical glycoproteins.
28953376	8	72	link	N-linked	1567:1574	arg1	glycan					1576:1581	an N-linked glycan	1564:1581	an N-linked glycan	1564:1581	Thus, we suggest that incorporating a Glu at either the -4 or +4 position relative to an N-linked glycan may be a useful strategy for engineering stable α-helical glycoproteins.
28953376	7	73	theme	glycopeptides	1309:1321	arg1	simulations					1278:1288	metadynamics simulations	1265:1288	metadynamics simulations of model α-helical glycopeptides with each of these two interactions	1265:1357	Via metadynamics simulations of model α-helical glycopeptides with each of these two interactions, we find that both interactions are stabilizing as a result of favorable electrostatic intraglycopeptide interactions.
28953376	3	74	theme	natural	755:761	arg1	sites					787:791	natural α-helical glycosylation sites	755:791	natural α-helical glycosylation sites in the Protein Data Bank	755:816	We identify two glycan conformations with an Asn χ1 of 180° or 300° that are amenable to α-helical structure in natural α-helical glycosylation sites in the Protein Data Bank.
28953376	7	75	with	glycopeptides	1309:1321	arg1	each					1328:1331	each	1328:1331	each	1328:1331	Via metadynamics simulations of model α-helical glycopeptides with each of these two interactions, we find that both interactions are stabilizing as a result of favorable electrostatic intraglycopeptide interactions.
28953376	7	75	with	glycopeptides	1309:1321	arg1	interactions					1346:1357	these two interactions	1336:1357	these two interactions	1336:1357	Via metadynamics simulations of model α-helical glycopeptides with each of these two interactions, we find that both interactions are stabilizing as a result of favorable electrostatic intraglycopeptide interactions.
28953376	4	76	gly	intraglycopeptide	858:874	arg2	intraglycopeptide					858:874	favorable intraglycopeptide enthalpy	848:883	favorable intraglycopeptide enthalpy	848:883	A combination of sterics and favorable intraglycopeptide enthalpy explains the existence of only these two conformations.
28953376	3	77	from	structure	742:750	arg1	sites					787:791	natural α-helical glycosylation sites	755:791	natural α-helical glycosylation sites in the Protein Data Bank	755:816	We identify two glycan conformations with an Asn χ1 of 180° or 300° that are amenable to α-helical structure in natural α-helical glycosylation sites in the Protein Data Bank.
28953376	2	78	theme	glycopeptides	442:454	arg1	design					422:427	the rational design	409:427	the rational design of α-helical glycopeptides	409:454	To further elucidate the effects of N-glycosylation on protein structure and to provide principles useful for the rational design of α-helical glycopeptides, we investigate stabilizing protein-sugar interactions in α-helical glycosylation sites using an integrated structural bioinformatics analysis and molecular dynamics simulation approach.
28953376	1	79	theme	therapeutic	279:289	arg1	design					291:296	therapeutic design	279:296	therapeutic design	279:296	Glycosylation not only impacts the functions of glycoproteins but can also improve glycoprotein stability and folding efficiency-characteristics that are desirable for protein engineering and therapeutic design.
28953376	2	80	theme	analysis	590:597	arg1	approach					633:640	an integrated structural bioinformatics analysis and molecular dynamics simulation approach	550:640	an integrated structural bioinformatics analysis and molecular dynamics simulation approach	550:640	To further elucidate the effects of N-glycosylation on protein structure and to provide principles useful for the rational design of α-helical glycopeptides, we investigate stabilizing protein-sugar interactions in α-helical glycosylation sites using an integrated structural bioinformatics analysis and molecular dynamics simulation approach.
28953376	3	81	theme	300°	706:709	arg1	χ1					692:693	an Asn χ1	685:693	an Asn χ1 of 180° or 300°	685:709	We identify two glycan conformations with an Asn χ1 of 180° or 300° that are amenable to α-helical structure in natural α-helical glycosylation sites in the Protein Data Bank.
28953376	3	82	theme	Protein	800:806	arg1	Bank					813:816	the Protein Data Bank	796:816	the Protein Data Bank	796:816	We identify two glycan conformations with an Asn χ1 of 180° or 300° that are amenable to α-helical structure in natural α-helical glycosylation sites in the Protein Data Bank.
28953376	2	83	theme	rational	413:420	arg1	design					422:427	the rational design	409:427	the rational design of α-helical glycopeptides	409:454	To further elucidate the effects of N-glycosylation on protein structure and to provide principles useful for the rational design of α-helical glycopeptides, we investigate stabilizing protein-sugar interactions in α-helical glycosylation sites using an integrated structural bioinformatics analysis and molecular dynamics simulation approach.
28953376	2	84	gly	N-glycosylation	335:349	arg1	protein					354:360	protein structure	354:370	protein structure	354:370	To further elucidate the effects of N-glycosylation on protein structure and to provide principles useful for the rational design of α-helical glycopeptides, we investigate stabilizing protein-sugar interactions in α-helical glycosylation sites using an integrated structural bioinformatics analysis and molecular dynamics simulation approach.
29406116	1	0	from	interest	175:182	arg1	years					140:144	recent years	133:144	recent years	133:144	In recent years there has been an increasing interest in the use of autohydrogenotrophic bacteria to treat nitrate from wastewater.
29406116	1	0	from	interest	175:182	arg1	use					191:193	the use	187:193	the use of autohydrogenotrophic bacteria to treat nitrate from wastewater	187:259	In recent years there has been an increasing interest in the use of autohydrogenotrophic bacteria to treat nitrate from wastewater.
29406116	4	1	theme	%	685:685	arg1	removal					696:702	100% nitrogen removal	682:702	100% nitrogen removal after 6hr of operation	682:725	Results showed that pH7.5 and a hydrogen flow rate of 90mL/min were the optimal operating conditions, resulting in 100% nitrogen removal after 6hr of operation.
29406116	5	2	theme	Soluble	728:734	arg1	polysaccharides					746:760	Soluble and bound polysaccharides	728:760	Soluble and bound polysaccharides	728:760	Soluble and bound polysaccharides decreased, while bound proteins increased with increasing pH. Polysaccharides increased with increasing hydrogen flow rate.
29406116	0	3	theme	various	94:100	arg1	pH					102:103	various pH	94:103	various pH	94:103	Nitrate removal and extracellular polymeric substances of autohydrogenotrophic bacteria under various pH and hydrogen flow rates.
29406116	6	4	theme	proteins	917:924	arg1	change					901:906	No significant change	886:906	No significant change of bound proteins	886:924	No significant change of bound proteins was observed at various hydrogen flow rates.
29406116	3	5	theme	hydrogen	502:509	arg1	rates					516:520	hydrogen flow rates	502:520	hydrogen flow rates	502:520	This study aimed to investigate the change in EPS compositions under various pH values and hydrogen flow rates, taking into consideration nitrogen removal.
29406116	0	6	theme	hydrogen	109:116	arg1	rates					123:127	hydrogen flow rates	109:127	hydrogen flow rates	109:127	Nitrate removal and extracellular polymeric substances of autohydrogenotrophic bacteria under various pH and hydrogen flow rates.
29406116	6	7	theme	bound	911:915	arg1	proteins					917:924	bound proteins	911:924	bound proteins	911:924	No significant change of bound proteins was observed at various hydrogen flow rates.
29406116	5	8	theme	bound	779:783	arg1	proteins					785:792	bound proteins	779:792	bound proteins	779:792	Soluble and bound polysaccharides decreased, while bound proteins increased with increasing pH. Polysaccharides increased with increasing hydrogen flow rate.
29406116	4	9	theme	operating	647:655	arg1	pH7.5					587:591	pH7.5	587:591	pH7.5	587:591	Results showed that pH7.5 and a hydrogen flow rate of 90mL/min were the optimal operating conditions, resulting in 100% nitrogen removal after 6hr of operation.
29406116	4	9	theme	operating	647:655	arg1	conditions					657:666	the optimal operating conditions	635:666	the optimal operating conditions	635:666	Results showed that pH7.5 and a hydrogen flow rate of 90mL/min were the optimal operating conditions, resulting in 100% nitrogen removal after 6hr of operation.
29406116	4	9	theme	operating	647:655	arg1	rate					613:616	a hydrogen flow rate	597:616	a hydrogen flow rate of 90mL/min	597:628	Results showed that pH7.5 and a hydrogen flow rate of 90mL/min were the optimal operating conditions, resulting in 100% nitrogen removal after 6hr of operation.
29406116	4	10	theme	operation	717:725	arg1	6hr					710:712	6hr	710:712	6hr of operation	710:725	Results showed that pH7.5 and a hydrogen flow rate of 90mL/min were the optimal operating conditions, resulting in 100% nitrogen removal after 6hr of operation.
29406116	2	11	theme	substances	338:347	arg1	characteristics					295:309	the characteristics	291:309	the characteristics of extracellular polymeric substances (EPS) releasing by these activities	291:383	However, our knowledge about the characteristics of extracellular polymeric substances (EPS) releasing by these activities is not yet very advanced.
29406116	1	12	theme	increasing	164:173	arg1	interest					175:182	an increasing interest	161:182	an increasing interest in the use of autohydrogenotrophic bacteria to treat nitrate from wastewater	161:259	In recent years there has been an increasing interest in the use of autohydrogenotrophic bacteria to treat nitrate from wastewater.
29406116	0	13	theme	flow	118:121	arg1	rates					123:127	hydrogen flow rates	109:127	hydrogen flow rates	109:127	Nitrate removal and extracellular polymeric substances of autohydrogenotrophic bacteria under various pH and hydrogen flow rates.
29406116	6	14	theme	significant	889:899	arg1	change					901:906	No significant change	886:906	No significant change of bound proteins	886:924	No significant change of bound proteins was observed at various hydrogen flow rates.
29406116	4	15	theme	optimal	639:645	arg1	pH7.5					587:591	pH7.5	587:591	pH7.5	587:591	Results showed that pH7.5 and a hydrogen flow rate of 90mL/min were the optimal operating conditions, resulting in 100% nitrogen removal after 6hr of operation.
29406116	4	15	theme	optimal	639:645	arg1	conditions					657:666	the optimal operating conditions	635:666	the optimal operating conditions	635:666	Results showed that pH7.5 and a hydrogen flow rate of 90mL/min were the optimal operating conditions, resulting in 100% nitrogen removal after 6hr of operation.
29406116	4	15	theme	optimal	639:645	arg1	rate					613:616	a hydrogen flow rate	597:616	a hydrogen flow rate of 90mL/min	597:628	Results showed that pH7.5 and a hydrogen flow rate of 90mL/min were the optimal operating conditions, resulting in 100% nitrogen removal after 6hr of operation.
29406116	2	16	theme	polymeric	328:336	arg1	EPS					350:352	EPS	350:352	EPS	350:352	However, our knowledge about the characteristics of extracellular polymeric substances (EPS) releasing by these activities is not yet very advanced.
29406116	2	16	theme	polymeric	328:336	arg1	substances					338:347	extracellular polymeric substances	314:347	extracellular polymeric substances (EPS) releasing by these activities	314:383	However, our knowledge about the characteristics of extracellular polymeric substances (EPS) releasing by these activities is not yet very advanced.
29406116	0	17	theme	Nitrate	0:6	arg1	removal					8:14	Nitrate removal	0:14	Nitrate removal	0:14	Nitrate removal and extracellular polymeric substances of autohydrogenotrophic bacteria under various pH and hydrogen flow rates.
29406116	5	18	theme	hydrogen	866:873	arg1	rate					880:883	hydrogen flow rate	866:883	hydrogen flow rate	866:883	Soluble and bound polysaccharides decreased, while bound proteins increased with increasing pH. Polysaccharides increased with increasing hydrogen flow rate.
29406116	2	19	theme	extracellular	314:326	arg1	EPS					350:352	EPS	350:352	EPS	350:352	However, our knowledge about the characteristics of extracellular polymeric substances (EPS) releasing by these activities is not yet very advanced.
29406116	2	19	theme	extracellular	314:326	arg1	substances					338:347	extracellular polymeric substances	314:347	extracellular polymeric substances (EPS) releasing by these activities	314:383	However, our knowledge about the characteristics of extracellular polymeric substances (EPS) releasing by these activities is not yet very advanced.
29406116	0	20	theme	extracellular	20:32	arg1	substances					44:53	extracellular polymeric substances	20:53	extracellular polymeric substances	20:53	Nitrate removal and extracellular polymeric substances of autohydrogenotrophic bacteria under various pH and hydrogen flow rates.
29406116	3	21	theme	nitrogen	549:556	arg1	removal					558:564	nitrogen removal	549:564	nitrogen removal	549:564	This study aimed to investigate the change in EPS compositions under various pH values and hydrogen flow rates, taking into consideration nitrogen removal.
29406116	3	22	theme	EPS	457:459	arg1	compositions					461:472	EPS compositions	457:472	EPS compositions	457:472	This study aimed to investigate the change in EPS compositions under various pH values and hydrogen flow rates, taking into consideration nitrogen removal.
29406116	5	23	theme	pH.	820:822	arg1	Polysaccharides					824:838	pH. Polysaccharides	820:838	pH. Polysaccharides increased with increasing hydrogen flow rate	820:883	Soluble and bound polysaccharides decreased, while bound proteins increased with increasing pH. Polysaccharides increased with increasing hydrogen flow rate.
29406116	4	24	theme	100	682:684	arg1	%					685:685	%	685:685	%	685:685	Results showed that pH7.5 and a hydrogen flow rate of 90mL/min were the optimal operating conditions, resulting in 100% nitrogen removal after 6hr of operation.
29406116	6	25	located	observed	930:937	arg1	rates					964:968	various hydrogen flow rates	942:968	various hydrogen flow rates	942:968	No significant change of bound proteins was observed at various hydrogen flow rates.
29406116	6	25	located	observed	930:937	arg2	change					901:906	No significant change	886:906	No significant change of bound proteins	886:924	No significant change of bound proteins was observed at various hydrogen flow rates.
29406116	1	26	theme	autohydrogenotrophic	198:217	arg1	bacteria					219:226	autohydrogenotrophic bacteria	198:226	autohydrogenotrophic bacteria	198:226	In recent years there has been an increasing interest in the use of autohydrogenotrophic bacteria to treat nitrate from wastewater.
29406116	0	27	theme	polymeric	34:42	arg1	substances					44:53	extracellular polymeric substances	20:53	extracellular polymeric substances	20:53	Nitrate removal and extracellular polymeric substances of autohydrogenotrophic bacteria under various pH and hydrogen flow rates.
29406116	6	28	theme	flow	959:962	arg1	rates					964:968	various hydrogen flow rates	942:968	various hydrogen flow rates	942:968	No significant change of bound proteins was observed at various hydrogen flow rates.
29406116	3	29	from	change	447:452	arg1	compositions					461:472	EPS compositions	457:472	EPS compositions	457:472	This study aimed to investigate the change in EPS compositions under various pH values and hydrogen flow rates, taking into consideration nitrogen removal.
29406116	1	30	theme	bacteria	219:226	arg1	use					191:193	the use	187:193	the use of autohydrogenotrophic bacteria to treat nitrate from wastewater	187:259	In recent years there has been an increasing interest in the use of autohydrogenotrophic bacteria to treat nitrate from wastewater.
29406116	0	31	theme	autohydrogenotrophic	58:77	arg1	bacteria					79:86	autohydrogenotrophic bacteria	58:86	autohydrogenotrophic bacteria	58:86	Nitrate removal and extracellular polymeric substances of autohydrogenotrophic bacteria under various pH and hydrogen flow rates.
29406116	6	32	theme	hydrogen	950:957	arg1	rates					964:968	various hydrogen flow rates	942:968	various hydrogen flow rates	942:968	No significant change of bound proteins was observed at various hydrogen flow rates.
29406116	5	33	theme	bound	740:744	arg1	polysaccharides					746:760	Soluble and bound polysaccharides	728:760	Soluble and bound polysaccharides	728:760	Soluble and bound polysaccharides decreased, while bound proteins increased with increasing pH. Polysaccharides increased with increasing hydrogen flow rate.
29406116	3	34	theme	pH	488:489	arg1	values					491:496	various pH values	480:496	various pH values	480:496	This study aimed to investigate the change in EPS compositions under various pH values and hydrogen flow rates, taking into consideration nitrogen removal.
29406116	6	35	theme	various	942:948	arg1	rates					964:968	various hydrogen flow rates	942:968	various hydrogen flow rates	942:968	No significant change of bound proteins was observed at various hydrogen flow rates.
29406116	4	36	theme	nitrogen	687:694	arg1	removal					696:702	100% nitrogen removal	682:702	100% nitrogen removal after 6hr of operation	682:725	Results showed that pH7.5 and a hydrogen flow rate of 90mL/min were the optimal operating conditions, resulting in 100% nitrogen removal after 6hr of operation.
29406116	1	37	theme	recent	133:138	arg1	years					140:144	recent years	133:144	recent years	133:144	In recent years there has been an increasing interest in the use of autohydrogenotrophic bacteria to treat nitrate from wastewater.
29406116	4	38	theme	hydrogen	599:606	arg1	pH7.5					587:591	pH7.5	587:591	pH7.5	587:591	Results showed that pH7.5 and a hydrogen flow rate of 90mL/min were the optimal operating conditions, resulting in 100% nitrogen removal after 6hr of operation.
29406116	4	38	theme	hydrogen	599:606	arg1	conditions					657:666	the optimal operating conditions	635:666	the optimal operating conditions	635:666	Results showed that pH7.5 and a hydrogen flow rate of 90mL/min were the optimal operating conditions, resulting in 100% nitrogen removal after 6hr of operation.
29406116	4	38	theme	hydrogen	599:606	arg1	rate					613:616	a hydrogen flow rate	597:616	a hydrogen flow rate of 90mL/min	597:628	Results showed that pH7.5 and a hydrogen flow rate of 90mL/min were the optimal operating conditions, resulting in 100% nitrogen removal after 6hr of operation.
29406116	3	39	theme	various	480:486	arg1	values					491:496	various pH values	480:496	various pH values	480:496	This study aimed to investigate the change in EPS compositions under various pH values and hydrogen flow rates, taking into consideration nitrogen removal.
29406116	4	40	theme	90mL/min	621:628	arg1	pH7.5					587:591	pH7.5	587:591	pH7.5	587:591	Results showed that pH7.5 and a hydrogen flow rate of 90mL/min were the optimal operating conditions, resulting in 100% nitrogen removal after 6hr of operation.
29406116	4	40	theme	90mL/min	621:628	arg1	conditions					657:666	the optimal operating conditions	635:666	the optimal operating conditions	635:666	Results showed that pH7.5 and a hydrogen flow rate of 90mL/min were the optimal operating conditions, resulting in 100% nitrogen removal after 6hr of operation.
29406116	4	40	theme	90mL/min	621:628	arg1	rate					613:616	a hydrogen flow rate	597:616	a hydrogen flow rate of 90mL/min	597:628	Results showed that pH7.5 and a hydrogen flow rate of 90mL/min were the optimal operating conditions, resulting in 100% nitrogen removal after 6hr of operation.
29406116	3	41	theme	flow	511:514	arg1	rates					516:520	hydrogen flow rates	502:520	hydrogen flow rates	502:520	This study aimed to investigate the change in EPS compositions under various pH values and hydrogen flow rates, taking into consideration nitrogen removal.
29406116	5	42	theme	flow	875:878	arg1	rate					880:883	hydrogen flow rate	866:883	hydrogen flow rate	866:883	Soluble and bound polysaccharides decreased, while bound proteins increased with increasing pH. Polysaccharides increased with increasing hydrogen flow rate.
29406116	0	43	theme	bacteria	79:86	arg1	substances					44:53	extracellular polymeric substances	20:53	extracellular polymeric substances	20:53	Nitrate removal and extracellular polymeric substances of autohydrogenotrophic bacteria under various pH and hydrogen flow rates.
29406116	0	43	theme	bacteria	79:86	arg1	removal					8:14	Nitrate removal	0:14	Nitrate removal	0:14	Nitrate removal and extracellular polymeric substances of autohydrogenotrophic bacteria under various pH and hydrogen flow rates.
29406116	4	44	theme	flow	608:611	arg1	pH7.5					587:591	pH7.5	587:591	pH7.5	587:591	Results showed that pH7.5 and a hydrogen flow rate of 90mL/min were the optimal operating conditions, resulting in 100% nitrogen removal after 6hr of operation.
29406116	4	44	theme	flow	608:611	arg1	conditions					657:666	the optimal operating conditions	635:666	the optimal operating conditions	635:666	Results showed that pH7.5 and a hydrogen flow rate of 90mL/min were the optimal operating conditions, resulting in 100% nitrogen removal after 6hr of operation.
29406116	4	44	theme	flow	608:611	arg1	rate					613:616	a hydrogen flow rate	597:616	a hydrogen flow rate of 90mL/min	597:628	Results showed that pH7.5 and a hydrogen flow rate of 90mL/min were the optimal operating conditions, resulting in 100% nitrogen removal after 6hr of operation.
27443590	0	0	from	optimization	21:32	arg1	date					67:70	date syrup	67:76	date syrup	67:76	Characterization and optimization of schizophyllan production from date syrup.
27443590	5	1	theme	glucose	1131:1137	arg1	residues					1139:1146	the 3-linked, 3,6-linked and terminal glucose residues	1093:1146	the 3-linked, 3,6-linked and terminal glucose residues	1093:1146	Sugar composition analysis, FTIR, NMR and molar mass determination revealed the purity and molecular properties of recovered schizophyllan produced from date syrup as glycosidic linkage analysis showed three main schizophyllan characteristic peaks arising from the 3-linked, 3,6-linked and terminal glucose residues.
27443590	1	2	theme	date	179:182	arg1	substrates					154:163	low-cost agricultural substrates	132:163	low-cost agricultural substrates	132:163	This study demonstrates the efficient utilization of low-cost agricultural substrates, particularly date syrup, by Schizophyllum commune ATCC 38548 for schizophyllan production.
27443590	1	2	theme	date	179:182	arg1	syrup					184:188	date syrup	179:188	particularly date syrup	166:188	This study demonstrates the efficient utilization of low-cost agricultural substrates, particularly date syrup, by Schizophyllum commune ATCC 38548 for schizophyllan production.
27443590	5	3	theme	3,6-linked	1107:1116	arg1	residues					1139:1146	the 3-linked, 3,6-linked and terminal glucose residues	1093:1146	the 3-linked, 3,6-linked and terminal glucose residues	1093:1146	Sugar composition analysis, FTIR, NMR and molar mass determination revealed the purity and molecular properties of recovered schizophyllan produced from date syrup as glycosidic linkage analysis showed three main schizophyllan characteristic peaks arising from the 3-linked, 3,6-linked and terminal glucose residues.
27443590	5	4	theme	recovered	947:955	arg1	schizophyllan					957:969	recovered schizophyllan	947:969	recovered schizophyllan produced from date syrup	947:994	Sugar composition analysis, FTIR, NMR and molar mass determination revealed the purity and molecular properties of recovered schizophyllan produced from date syrup as glycosidic linkage analysis showed three main schizophyllan characteristic peaks arising from the 3-linked, 3,6-linked and terminal glucose residues.
27443590	5	5	theme	mass	880:883	arg1	determination					885:897	molar mass determination	874:897	molar mass determination	874:897	Sugar composition analysis, FTIR, NMR and molar mass determination revealed the purity and molecular properties of recovered schizophyllan produced from date syrup as glycosidic linkage analysis showed three main schizophyllan characteristic peaks arising from the 3-linked, 3,6-linked and terminal glucose residues.
27443590	3	6	theme	submerged	558:566	arg1	culture					568:574	submerged culture	558:574	submerged culture	558:574	Subsequently, response surface methodology was employed to optimize the level of culture medium components to maximize substrate conversion yield and schizophyllan production in submerged culture.
27443590	5	7	theme	molecular	923:931	arg1	properties					933:942	molecular properties	923:942	molecular properties	923:942	Sugar composition analysis, FTIR, NMR and molar mass determination revealed the purity and molecular properties of recovered schizophyllan produced from date syrup as glycosidic linkage analysis showed three main schizophyllan characteristic peaks arising from the 3-linked, 3,6-linked and terminal glucose residues.
27443590	6	8	theme	nitrogen	1392:1399	arg1	extract					1434:1440	malt extract	1429:1440	malt extract	1429:1440	Finally, process economic analysis suggested that use of date syrup and corn steep liquor as nutrients would result in approximately 6-fold reduction in cost of raw materials for schizophyllan production as compared to conventional carbon and nitrogen sources such as sucrose and malt extract.
27443590	6	8	theme	nitrogen	1392:1399	arg1	sources					1401:1407	conventional carbon and nitrogen sources	1368:1407	sources	1401:1407	Finally, process economic analysis suggested that use of date syrup and corn steep liquor as nutrients would result in approximately 6-fold reduction in cost of raw materials for schizophyllan production as compared to conventional carbon and nitrogen sources such as sucrose and malt extract.
27443590	6	8	theme	nitrogen	1392:1399	arg1	sucrose					1417:1423	sucrose	1417:1423	sucrose	1417:1423	Finally, process economic analysis suggested that use of date syrup and corn steep liquor as nutrients would result in approximately 6-fold reduction in cost of raw materials for schizophyllan production as compared to conventional carbon and nitrogen sources such as sucrose and malt extract.
27443590	0	9	from	Characterization	0:15	arg1	date					67:70	date syrup	67:76	date syrup	67:76	Characterization and optimization of schizophyllan production from date syrup.
27443590	5	10	theme	schizophyllan	957:969	arg1	purity					912:917	purity	912:917	purity	912:917	Sugar composition analysis, FTIR, NMR and molar mass determination revealed the purity and molecular properties of recovered schizophyllan produced from date syrup as glycosidic linkage analysis showed three main schizophyllan characteristic peaks arising from the 3-linked, 3,6-linked and terminal glucose residues.
27443590	5	10	theme	schizophyllan	957:969	arg1	properties					933:942	molecular properties	923:942	molecular properties	923:942	Sugar composition analysis, FTIR, NMR and molar mass determination revealed the purity and molecular properties of recovered schizophyllan produced from date syrup as glycosidic linkage analysis showed three main schizophyllan characteristic peaks arising from the 3-linked, 3,6-linked and terminal glucose residues.
27443590	4	11	theme	syrup	712:716	arg1	concentrations					689:702	concentrations	689:702	concentrations of date syrup and corn steep liquor	689:738	Maximum product yield (0.12g schizophyllan/g date syrup) and schizophyllan production (8.5g/l) were obtained at concentrations of date syrup and corn steep liquor, inoculum size and agitation rate at 7.02%w/v, 0.10%w/v, 7.68%v/v and 181rpm, respectively.
27443590	4	11	theme	syrup	712:716	arg1	size					750:753	inoculum size	741:753	inoculum size	741:753	Maximum product yield (0.12g schizophyllan/g date syrup) and schizophyllan production (8.5g/l) were obtained at concentrations of date syrup and corn steep liquor, inoculum size and agitation rate at 7.02%w/v, 0.10%w/v, 7.68%v/v and 181rpm, respectively.
27443590	4	11	theme	syrup	712:716	arg1	rate					769:772	agitation rate	759:772	agitation rate	759:772	Maximum product yield (0.12g schizophyllan/g date syrup) and schizophyllan production (8.5g/l) were obtained at concentrations of date syrup and corn steep liquor, inoculum size and agitation rate at 7.02%w/v, 0.10%w/v, 7.68%v/v and 181rpm, respectively.
27443590	6	12	from	reduction	1289:1297	arg1	cost					1302:1305	cost	1302:1305	cost of raw materials for schizophyllan production	1302:1351	Finally, process economic analysis suggested that use of date syrup and corn steep liquor as nutrients would result in approximately 6-fold reduction in cost of raw materials for schizophyllan production as compared to conventional carbon and nitrogen sources such as sucrose and malt extract.
27443590	3	13	theme	response	394:401	arg1	methodology					411:421	response surface methodology	394:421	response surface methodology	394:421	Subsequently, response surface methodology was employed to optimize the level of culture medium components to maximize substrate conversion yield and schizophyllan production in submerged culture.
27443590	0	14	from	date	67:70	arg1	optimization					21:32	optimization	21:32	optimization	21:32	Characterization and optimization of schizophyllan production from date syrup.
27443590	0	14	from	date	67:70	arg1	production					51:60	schizophyllan production	37:60	schizophyllan production from date syrup	37:76	Characterization and optimization of schizophyllan production from date syrup.
27443590	0	14	from	date	67:70	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and optimization of schizophyllan production from date syrup.
27443590	6	15	theme	corn	1221:1224	arg1	liquor					1232:1237	corn steep liquor	1221:1237	corn steep liquor	1221:1237	Finally, process economic analysis suggested that use of date syrup and corn steep liquor as nutrients would result in approximately 6-fold reduction in cost of raw materials for schizophyllan production as compared to conventional carbon and nitrogen sources such as sucrose and malt extract.
27443590	4	16	theme	inoculum	741:748	arg1	size					750:753	inoculum size	741:753	inoculum size	741:753	Maximum product yield (0.12g schizophyllan/g date syrup) and schizophyllan production (8.5g/l) were obtained at concentrations of date syrup and corn steep liquor, inoculum size and agitation rate at 7.02%w/v, 0.10%w/v, 7.68%v/v and 181rpm, respectively.
27443590	4	17	theme	agitation	759:767	arg1	rate					769:772	agitation rate	759:772	agitation rate	759:772	Maximum product yield (0.12g schizophyllan/g date syrup) and schizophyllan production (8.5g/l) were obtained at concentrations of date syrup and corn steep liquor, inoculum size and agitation rate at 7.02%w/v, 0.10%w/v, 7.68%v/v and 181rpm, respectively.
27443590	6	18	theme	6-fold	1282:1287	arg1	reduction					1289:1297	approximately 6-fold reduction	1268:1297	approximately 6-fold reduction in cost of raw materials for schizophyllan production	1268:1351	Finally, process economic analysis suggested that use of date syrup and corn steep liquor as nutrients would result in approximately 6-fold reduction in cost of raw materials for schizophyllan production as compared to conventional carbon and nitrogen sources such as sucrose and malt extract.
27443590	5	19	theme	Sugar	832:836	arg1	analysis					850:857	Sugar composition analysis	832:857	Sugar composition analysis	832:857	Sugar composition analysis, FTIR, NMR and molar mass determination revealed the purity and molecular properties of recovered schizophyllan produced from date syrup as glycosidic linkage analysis showed three main schizophyllan characteristic peaks arising from the 3-linked, 3,6-linked and terminal glucose residues.
27443590	4	20	theme	corn	722:725	arg1	liquor					733:738	corn steep liquor	722:738	corn steep liquor	722:738	Maximum product yield (0.12g schizophyllan/g date syrup) and schizophyllan production (8.5g/l) were obtained at concentrations of date syrup and corn steep liquor, inoculum size and agitation rate at 7.02%w/v, 0.10%w/v, 7.68%v/v and 181rpm, respectively.
27443590	3	21	theme	medium	469:474	arg1	components					476:485	culture medium components	461:485	culture medium components	461:485	Subsequently, response surface methodology was employed to optimize the level of culture medium components to maximize substrate conversion yield and schizophyllan production in submerged culture.
27443590	4	22	theme	schizophyllan	638:650	arg1	8.5g/l					664:669	8.5g/l	664:669	8.5g/l	664:669	Maximum product yield (0.12g schizophyllan/g date syrup) and schizophyllan production (8.5g/l) were obtained at concentrations of date syrup and corn steep liquor, inoculum size and agitation rate at 7.02%w/v, 0.10%w/v, 7.68%v/v and 181rpm, respectively.
27443590	4	22	theme	schizophyllan	638:650	arg1	production					652:661	schizophyllan production	638:661	schizophyllan production (8.5g/l)	638:670	Maximum product yield (0.12g schizophyllan/g date syrup) and schizophyllan production (8.5g/l) were obtained at concentrations of date syrup and corn steep liquor, inoculum size and agitation rate at 7.02%w/v, 0.10%w/v, 7.68%v/v and 181rpm, respectively.
27443590	5	23	theme	terminal	1122:1129	arg1	residues					1139:1146	the 3-linked, 3,6-linked and terminal glucose residues	1093:1146	the 3-linked, 3,6-linked and terminal glucose residues	1093:1146	Sugar composition analysis, FTIR, NMR and molar mass determination revealed the purity and molecular properties of recovered schizophyllan produced from date syrup as glycosidic linkage analysis showed three main schizophyllan characteristic peaks arising from the 3-linked, 3,6-linked and terminal glucose residues.
27443590	6	24	theme	conventional	1368:1379	arg1	carbon					1381:1386	conventional carbon and nitrogen sources	1368:1407	carbon	1381:1386	Finally, process economic analysis suggested that use of date syrup and corn steep liquor as nutrients would result in approximately 6-fold reduction in cost of raw materials for schizophyllan production as compared to conventional carbon and nitrogen sources such as sucrose and malt extract.
27443590	3	25	from	yield	520:524	arg1	culture					568:574	submerged culture	558:574	submerged culture	558:574	Subsequently, response surface methodology was employed to optimize the level of culture medium components to maximize substrate conversion yield and schizophyllan production in submerged culture.
27443590	5	26	theme	date	985:988	arg1	syrup					990:994	date syrup	985:994	date syrup	985:994	Sugar composition analysis, FTIR, NMR and molar mass determination revealed the purity and molecular properties of recovered schizophyllan produced from date syrup as glycosidic linkage analysis showed three main schizophyllan characteristic peaks arising from the 3-linked, 3,6-linked and terminal glucose residues.
27443590	5	27	link	3,6-linked	1107:1116	arg1	residues					1139:1146	the 3-linked, 3,6-linked and terminal glucose residues	1093:1146	the 3-linked, 3,6-linked and terminal glucose residues	1093:1146	Sugar composition analysis, FTIR, NMR and molar mass determination revealed the purity and molecular properties of recovered schizophyllan produced from date syrup as glycosidic linkage analysis showed three main schizophyllan characteristic peaks arising from the 3-linked, 3,6-linked and terminal glucose residues.
27443590	3	28	theme	components	476:485	arg1	level					452:456	the level	448:456	the level of culture medium components to maximize substrate conversion yield and schizophyllan production in submerged culture	448:574	Subsequently, response surface methodology was employed to optimize the level of culture medium components to maximize substrate conversion yield and schizophyllan production in submerged culture.
27443590	4	29	theme	date	622:625	arg1	yield					593:597	Maximum product yield	577:597	Maximum product yield (0.12g schizophyllan/g date syrup)	577:632	Maximum product yield (0.12g schizophyllan/g date syrup) and schizophyllan production (8.5g/l) were obtained at concentrations of date syrup and corn steep liquor, inoculum size and agitation rate at 7.02%w/v, 0.10%w/v, 7.68%v/v and 181rpm, respectively.
27443590	4	29	theme	date	622:625	arg1	syrup					627:631	0.12g schizophyllan/g date syrup	600:631	0.12g schizophyllan/g date syrup	600:631	Maximum product yield (0.12g schizophyllan/g date syrup) and schizophyllan production (8.5g/l) were obtained at concentrations of date syrup and corn steep liquor, inoculum size and agitation rate at 7.02%w/v, 0.10%w/v, 7.68%v/v and 181rpm, respectively.
27443590	5	30	link	3-linked	1097:1104	arg1	residues					1139:1146	the 3-linked, 3,6-linked and terminal glucose residues	1093:1146	the 3-linked, 3,6-linked and terminal glucose residues	1093:1146	Sugar composition analysis, FTIR, NMR and molar mass determination revealed the purity and molecular properties of recovered schizophyllan produced from date syrup as glycosidic linkage analysis showed three main schizophyllan characteristic peaks arising from the 3-linked, 3,6-linked and terminal glucose residues.
27443590	2	31	theme	nitrogen	333:340	arg1	sources					342:348	carbon and nitrogen sources	322:348	sources	342:348	Initially, one factor-at-a-time method was used to find the best carbon and nitrogen sources for schizophyllan production.
27443590	4	32	dep	%	791:791	arg1	w/v					792:794	w/v	792:794	0.10%w/v	787:794	Maximum product yield (0.12g schizophyllan/g date syrup) and schizophyllan production (8.5g/l) were obtained at concentrations of date syrup and corn steep liquor, inoculum size and agitation rate at 7.02%w/v, 0.10%w/v, 7.68%v/v and 181rpm, respectively.
27443590	1	33	theme	Schizophyllum	194:206	arg1	38548					221:225	Schizophyllum commune ATCC 38548	194:225	Schizophyllum commune ATCC 38548 for schizophyllan production	194:254	This study demonstrates the efficient utilization of low-cost agricultural substrates, particularly date syrup, by Schizophyllum commune ATCC 38548 for schizophyllan production.
27443590	6	34	theme	economic	1166:1173	arg1	analysis					1175:1182	process economic analysis	1158:1182	process economic analysis	1158:1182	Finally, process economic analysis suggested that use of date syrup and corn steep liquor as nutrients would result in approximately 6-fold reduction in cost of raw materials for schizophyllan production as compared to conventional carbon and nitrogen sources such as sucrose and malt extract.
27443590	4	35	theme	schizophyllan/g	606:620	arg1	yield					593:597	Maximum product yield	577:597	Maximum product yield (0.12g schizophyllan/g date syrup)	577:632	Maximum product yield (0.12g schizophyllan/g date syrup) and schizophyllan production (8.5g/l) were obtained at concentrations of date syrup and corn steep liquor, inoculum size and agitation rate at 7.02%w/v, 0.10%w/v, 7.68%v/v and 181rpm, respectively.
27443590	4	35	theme	schizophyllan/g	606:620	arg1	syrup					627:631	0.12g schizophyllan/g date syrup	600:631	0.12g schizophyllan/g date syrup	600:631	Maximum product yield (0.12g schizophyllan/g date syrup) and schizophyllan production (8.5g/l) were obtained at concentrations of date syrup and corn steep liquor, inoculum size and agitation rate at 7.02%w/v, 0.10%w/v, 7.68%v/v and 181rpm, respectively.
27443590	3	36	theme	culture	461:467	arg1	components					476:485	culture medium components	461:485	culture medium components	461:485	Subsequently, response surface methodology was employed to optimize the level of culture medium components to maximize substrate conversion yield and schizophyllan production in submerged culture.
27443590	3	37	theme	surface	403:409	arg1	methodology					411:421	response surface methodology	394:421	response surface methodology	394:421	Subsequently, response surface methodology was employed to optimize the level of culture medium components to maximize substrate conversion yield and schizophyllan production in submerged culture.
27443590	4	38	theme	0.12g	600:604	arg1	yield					593:597	Maximum product yield	577:597	Maximum product yield (0.12g schizophyllan/g date syrup)	577:632	Maximum product yield (0.12g schizophyllan/g date syrup) and schizophyllan production (8.5g/l) were obtained at concentrations of date syrup and corn steep liquor, inoculum size and agitation rate at 7.02%w/v, 0.10%w/v, 7.68%v/v and 181rpm, respectively.
27443590	4	38	theme	0.12g	600:604	arg1	syrup					627:631	0.12g schizophyllan/g date syrup	600:631	0.12g schizophyllan/g date syrup	600:631	Maximum product yield (0.12g schizophyllan/g date syrup) and schizophyllan production (8.5g/l) were obtained at concentrations of date syrup and corn steep liquor, inoculum size and agitation rate at 7.02%w/v, 0.10%w/v, 7.68%v/v and 181rpm, respectively.
27443590	5	39	theme	glycosidic	999:1008	arg1	analysis					1018:1025	glycosidic linkage analysis	999:1025	glycosidic linkage analysis	999:1025	Sugar composition analysis, FTIR, NMR and molar mass determination revealed the purity and molecular properties of recovered schizophyllan produced from date syrup as glycosidic linkage analysis showed three main schizophyllan characteristic peaks arising from the 3-linked, 3,6-linked and terminal glucose residues.
27443590	3	40	from	production	544:553	arg1	culture					568:574	submerged culture	558:574	submerged culture	558:574	Subsequently, response surface methodology was employed to optimize the level of culture medium components to maximize substrate conversion yield and schizophyllan production in submerged culture.
27443590	2	41	theme	carbon	322:327	arg1	sources					342:348	carbon and nitrogen sources	322:348	sources	342:348	Initially, one factor-at-a-time method was used to find the best carbon and nitrogen sources for schizophyllan production.
27443590	1	42	theme	ATCC	216:219	arg1	38548					221:225	Schizophyllum commune ATCC 38548	194:225	Schizophyllum commune ATCC 38548 for schizophyllan production	194:254	This study demonstrates the efficient utilization of low-cost agricultural substrates, particularly date syrup, by Schizophyllum commune ATCC 38548 for schizophyllan production.
27443590	6	43	theme	date	1206:1209	arg1	syrup					1211:1215	date syrup	1206:1215	date syrup	1206:1215	Finally, process economic analysis suggested that use of date syrup and corn steep liquor as nutrients would result in approximately 6-fold reduction in cost of raw materials for schizophyllan production as compared to conventional carbon and nitrogen sources such as sucrose and malt extract.
27443590	2	44	theme	best	317:320	arg1	sources					342:348	carbon and nitrogen sources	322:348	sources	342:348	Initially, one factor-at-a-time method was used to find the best carbon and nitrogen sources for schizophyllan production.
27443590	3	45	theme	conversion	509:518	arg1	yield					520:524	substrate conversion yield	499:524	substrate conversion yield	499:524	Subsequently, response surface methodology was employed to optimize the level of culture medium components to maximize substrate conversion yield and schizophyllan production in submerged culture.
27443590	0	46	theme	production	51:60	arg1	optimization					21:32	optimization	21:32	optimization	21:32	Characterization and optimization of schizophyllan production from date syrup.
27443590	0	46	theme	production	51:60	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and optimization of schizophyllan production from date syrup.
27443590	2	47	theme	schizophyllan	354:366	arg1	production					368:377	schizophyllan production	354:377	schizophyllan production	354:377	Initially, one factor-at-a-time method was used to find the best carbon and nitrogen sources for schizophyllan production.
27443590	1	48	theme	commune	208:214	arg1	38548					221:225	Schizophyllum commune ATCC 38548	194:225	Schizophyllum commune ATCC 38548 for schizophyllan production	194:254	This study demonstrates the efficient utilization of low-cost agricultural substrates, particularly date syrup, by Schizophyllum commune ATCC 38548 for schizophyllan production.
27443590	5	49	theme	composition	838:848	arg1	analysis					850:857	Sugar composition analysis	832:857	Sugar composition analysis	832:857	Sugar composition analysis, FTIR, NMR and molar mass determination revealed the purity and molecular properties of recovered schizophyllan produced from date syrup as glycosidic linkage analysis showed three main schizophyllan characteristic peaks arising from the 3-linked, 3,6-linked and terminal glucose residues.
27443590	0	50	theme	schizophyllan	37:49	arg1	production					51:60	schizophyllan production	37:60	schizophyllan production from date syrup	37:76	Characterization and optimization of schizophyllan production from date syrup.
27443590	4	51	theme	product	585:591	arg1	yield					593:597	Maximum product yield	577:597	Maximum product yield (0.12g schizophyllan/g date syrup)	577:632	Maximum product yield (0.12g schizophyllan/g date syrup) and schizophyllan production (8.5g/l) were obtained at concentrations of date syrup and corn steep liquor, inoculum size and agitation rate at 7.02%w/v, 0.10%w/v, 7.68%v/v and 181rpm, respectively.
27443590	4	51	theme	product	585:591	arg1	syrup					627:631	0.12g schizophyllan/g date syrup	600:631	0.12g schizophyllan/g date syrup	600:631	Maximum product yield (0.12g schizophyllan/g date syrup) and schizophyllan production (8.5g/l) were obtained at concentrations of date syrup and corn steep liquor, inoculum size and agitation rate at 7.02%w/v, 0.10%w/v, 7.68%v/v and 181rpm, respectively.
27443590	5	52	theme	3-linked	1097:1104	arg1	residues					1139:1146	the 3-linked, 3,6-linked and terminal glucose residues	1093:1146	the 3-linked, 3,6-linked and terminal glucose residues	1093:1146	Sugar composition analysis, FTIR, NMR and molar mass determination revealed the purity and molecular properties of recovered schizophyllan produced from date syrup as glycosidic linkage analysis showed three main schizophyllan characteristic peaks arising from the 3-linked, 3,6-linked and terminal glucose residues.
27443590	1	53	theme	schizophyllan	231:243	arg1	production					245:254	schizophyllan production	231:254	schizophyllan production	231:254	This study demonstrates the efficient utilization of low-cost agricultural substrates, particularly date syrup, by Schizophyllum commune ATCC 38548 for schizophyllan production.
27443590	6	54	theme	malt	1429:1432	arg1	extract					1434:1440	malt extract	1429:1440	malt extract	1429:1440	Finally, process economic analysis suggested that use of date syrup and corn steep liquor as nutrients would result in approximately 6-fold reduction in cost of raw materials for schizophyllan production as compared to conventional carbon and nitrogen sources such as sucrose and malt extract.
27443590	4	55	theme	Maximum	577:583	arg1	yield					593:597	Maximum product yield	577:597	Maximum product yield (0.12g schizophyllan/g date syrup)	577:632	Maximum product yield (0.12g schizophyllan/g date syrup) and schizophyllan production (8.5g/l) were obtained at concentrations of date syrup and corn steep liquor, inoculum size and agitation rate at 7.02%w/v, 0.10%w/v, 7.68%v/v and 181rpm, respectively.
27443590	4	55	theme	Maximum	577:583	arg1	syrup					627:631	0.12g schizophyllan/g date syrup	600:631	0.12g schizophyllan/g date syrup	600:631	Maximum product yield (0.12g schizophyllan/g date syrup) and schizophyllan production (8.5g/l) were obtained at concentrations of date syrup and corn steep liquor, inoculum size and agitation rate at 7.02%w/v, 0.10%w/v, 7.68%v/v and 181rpm, respectively.
27443590	6	56	theme	schizophyllan	1328:1340	arg1	production					1342:1351	schizophyllan production	1328:1351	schizophyllan production	1328:1351	Finally, process economic analysis suggested that use of date syrup and corn steep liquor as nutrients would result in approximately 6-fold reduction in cost of raw materials for schizophyllan production as compared to conventional carbon and nitrogen sources such as sucrose and malt extract.
27443590	3	57	theme	schizophyllan	530:542	arg1	production					544:553	schizophyllan production	530:553	schizophyllan production in submerged culture	530:574	Subsequently, response surface methodology was employed to optimize the level of culture medium components to maximize substrate conversion yield and schizophyllan production in submerged culture.
27443590	5	58	theme	schizophyllan	1045:1057	arg1	peaks					1074:1078	three main schizophyllan characteristic peaks	1034:1078	three main schizophyllan characteristic peaks arising from the 3-linked, 3,6-linked and terminal glucose residues	1034:1146	Sugar composition analysis, FTIR, NMR and molar mass determination revealed the purity and molecular properties of recovered schizophyllan produced from date syrup as glycosidic linkage analysis showed three main schizophyllan characteristic peaks arising from the 3-linked, 3,6-linked and terminal glucose residues.
27443590	5	59	theme	main	1040:1043	arg1	peaks					1074:1078	three main schizophyllan characteristic peaks	1034:1078	three main schizophyllan characteristic peaks arising from the 3-linked, 3,6-linked and terminal glucose residues	1034:1146	Sugar composition analysis, FTIR, NMR and molar mass determination revealed the purity and molecular properties of recovered schizophyllan produced from date syrup as glycosidic linkage analysis showed three main schizophyllan characteristic peaks arising from the 3-linked, 3,6-linked and terminal glucose residues.
27443590	1	60	theme	low-cost	132:139	arg1	substrates					154:163	low-cost agricultural substrates	132:163	low-cost agricultural substrates	132:163	This study demonstrates the efficient utilization of low-cost agricultural substrates, particularly date syrup, by Schizophyllum commune ATCC 38548 for schizophyllan production.
27443590	1	60	theme	low-cost	132:139	arg1	syrup					184:188	date syrup	179:188	particularly date syrup	166:188	This study demonstrates the efficient utilization of low-cost agricultural substrates, particularly date syrup, by Schizophyllum commune ATCC 38548 for schizophyllan production.
27443590	6	61	theme	liquor	1232:1237	arg1	use					1199:1201	use	1199:1201	use of date syrup and corn steep liquor as nutrients	1199:1250	Finally, process economic analysis suggested that use of date syrup and corn steep liquor as nutrients would result in approximately 6-fold reduction in cost of raw materials for schizophyllan production as compared to conventional carbon and nitrogen sources such as sucrose and malt extract.
27443590	5	62	theme	linkage	1010:1016	arg1	analysis					1018:1025	glycosidic linkage analysis	999:1025	glycosidic linkage analysis	999:1025	Sugar composition analysis, FTIR, NMR and molar mass determination revealed the purity and molecular properties of recovered schizophyllan produced from date syrup as glycosidic linkage analysis showed three main schizophyllan characteristic peaks arising from the 3-linked, 3,6-linked and terminal glucose residues.
27443590	6	63	theme	materials	1314:1322	arg1	cost					1302:1305	cost	1302:1305	cost of raw materials for schizophyllan production	1302:1351	Finally, process economic analysis suggested that use of date syrup and corn steep liquor as nutrients would result in approximately 6-fold reduction in cost of raw materials for schizophyllan production as compared to conventional carbon and nitrogen sources such as sucrose and malt extract.
27443590	5	64	theme	characteristic	1059:1072	arg1	peaks					1074:1078	three main schizophyllan characteristic peaks	1034:1078	three main schizophyllan characteristic peaks arising from the 3-linked, 3,6-linked and terminal glucose residues	1034:1146	Sugar composition analysis, FTIR, NMR and molar mass determination revealed the purity and molecular properties of recovered schizophyllan produced from date syrup as glycosidic linkage analysis showed three main schizophyllan characteristic peaks arising from the 3-linked, 3,6-linked and terminal glucose residues.
27443590	4	65	theme	7.68	797:800	arg1	%					801:801	%	801:801	%	801:801	Maximum product yield (0.12g schizophyllan/g date syrup) and schizophyllan production (8.5g/l) were obtained at concentrations of date syrup and corn steep liquor, inoculum size and agitation rate at 7.02%w/v, 0.10%w/v, 7.68%v/v and 181rpm, respectively.
27443590	1	66	theme	agricultural	141:152	arg1	substrates					154:163	low-cost agricultural substrates	132:163	low-cost agricultural substrates	132:163	This study demonstrates the efficient utilization of low-cost agricultural substrates, particularly date syrup, by Schizophyllum commune ATCC 38548 for schizophyllan production.
27443590	1	66	theme	agricultural	141:152	arg1	syrup					184:188	date syrup	179:188	particularly date syrup	166:188	This study demonstrates the efficient utilization of low-cost agricultural substrates, particularly date syrup, by Schizophyllum commune ATCC 38548 for schizophyllan production.
27443590	1	67	theme	efficient	107:115	arg1	utilization					117:127	the efficient utilization	103:127	the efficient utilization of low-cost agricultural substrates, particularly date syrup, by Schizophyllum commune ATCC 38548 for schizophyllan production	103:254	This study demonstrates the efficient utilization of low-cost agricultural substrates, particularly date syrup, by Schizophyllum commune ATCC 38548 for schizophyllan production.
27443590	6	68	theme	raw	1310:1312	arg1	materials					1314:1322	raw materials	1310:1322	raw materials	1310:1322	Finally, process economic analysis suggested that use of date syrup and corn steep liquor as nutrients would result in approximately 6-fold reduction in cost of raw materials for schizophyllan production as compared to conventional carbon and nitrogen sources such as sucrose and malt extract.
27443590	6	69	theme	steep	1226:1230	arg1	liquor					1232:1237	corn steep liquor	1221:1237	corn steep liquor	1221:1237	Finally, process economic analysis suggested that use of date syrup and corn steep liquor as nutrients would result in approximately 6-fold reduction in cost of raw materials for schizophyllan production as compared to conventional carbon and nitrogen sources such as sucrose and malt extract.
27443590	5	70	dep	purity	912:917	arg1	the					908:910	the	908:910	the	908:910	Sugar composition analysis, FTIR, NMR and molar mass determination revealed the purity and molecular properties of recovered schizophyllan produced from date syrup as glycosidic linkage analysis showed three main schizophyllan characteristic peaks arising from the 3-linked, 3,6-linked and terminal glucose residues.
27443590	1	71	theme	substrates	154:163	arg1	utilization					117:127	the efficient utilization	103:127	the efficient utilization of low-cost agricultural substrates, particularly date syrup, by Schizophyllum commune ATCC 38548 for schizophyllan production	103:254	This study demonstrates the efficient utilization of low-cost agricultural substrates, particularly date syrup, by Schizophyllum commune ATCC 38548 for schizophyllan production.
27443590	6	72	theme	syrup	1211:1215	arg1	use					1199:1201	use	1199:1201	use of date syrup and corn steep liquor as nutrients	1199:1250	Finally, process economic analysis suggested that use of date syrup and corn steep liquor as nutrients would result in approximately 6-fold reduction in cost of raw materials for schizophyllan production as compared to conventional carbon and nitrogen sources such as sucrose and malt extract.
27443590	4	73	dep	%	781:781	arg1	w/v					782:784	w/v	782:784	7.02%w/v	777:784	Maximum product yield (0.12g schizophyllan/g date syrup) and schizophyllan production (8.5g/l) were obtained at concentrations of date syrup and corn steep liquor, inoculum size and agitation rate at 7.02%w/v, 0.10%w/v, 7.68%v/v and 181rpm, respectively.
27443590	5	74	theme	molar	874:878	arg1	determination					885:897	molar mass determination	874:897	molar mass determination	874:897	Sugar composition analysis, FTIR, NMR and molar mass determination revealed the purity and molecular properties of recovered schizophyllan produced from date syrup as glycosidic linkage analysis showed three main schizophyllan characteristic peaks arising from the 3-linked, 3,6-linked and terminal glucose residues.
27443590	2	75	theme	factor-at-a-time	272:287	arg1	method					289:294	one factor-at-a-time method	268:294	one factor-at-a-time method	268:294	Initially, one factor-at-a-time method was used to find the best carbon and nitrogen sources for schizophyllan production.
27443590	3	76	theme	substrate	499:507	arg1	yield					520:524	substrate conversion yield	499:524	substrate conversion yield	499:524	Subsequently, response surface methodology was employed to optimize the level of culture medium components to maximize substrate conversion yield and schizophyllan production in submerged culture.
27443590	4	77	theme	liquor	733:738	arg1	concentrations					689:702	concentrations	689:702	concentrations of date syrup and corn steep liquor	689:738	Maximum product yield (0.12g schizophyllan/g date syrup) and schizophyllan production (8.5g/l) were obtained at concentrations of date syrup and corn steep liquor, inoculum size and agitation rate at 7.02%w/v, 0.10%w/v, 7.68%v/v and 181rpm, respectively.
27443590	4	77	theme	liquor	733:738	arg1	size					750:753	inoculum size	741:753	inoculum size	741:753	Maximum product yield (0.12g schizophyllan/g date syrup) and schizophyllan production (8.5g/l) were obtained at concentrations of date syrup and corn steep liquor, inoculum size and agitation rate at 7.02%w/v, 0.10%w/v, 7.68%v/v and 181rpm, respectively.
27443590	4	77	theme	liquor	733:738	arg1	rate					769:772	agitation rate	759:772	agitation rate	759:772	Maximum product yield (0.12g schizophyllan/g date syrup) and schizophyllan production (8.5g/l) were obtained at concentrations of date syrup and corn steep liquor, inoculum size and agitation rate at 7.02%w/v, 0.10%w/v, 7.68%v/v and 181rpm, respectively.
27443590	4	78	theme	date	707:710	arg1	syrup					712:716	date syrup	707:716	date syrup	707:716	Maximum product yield (0.12g schizophyllan/g date syrup) and schizophyllan production (8.5g/l) were obtained at concentrations of date syrup and corn steep liquor, inoculum size and agitation rate at 7.02%w/v, 0.10%w/v, 7.68%v/v and 181rpm, respectively.
27443590	6	79	theme	process	1158:1164	arg1	analysis					1175:1182	process economic analysis	1158:1182	process economic analysis	1158:1182	Finally, process economic analysis suggested that use of date syrup and corn steep liquor as nutrients would result in approximately 6-fold reduction in cost of raw materials for schizophyllan production as compared to conventional carbon and nitrogen sources such as sucrose and malt extract.
27443590	4	80	theme	%	801:801	arg1	v/v					802:804	7.68%v/v	797:804	7.68%v/v	797:804	Maximum product yield (0.12g schizophyllan/g date syrup) and schizophyllan production (8.5g/l) were obtained at concentrations of date syrup and corn steep liquor, inoculum size and agitation rate at 7.02%w/v, 0.10%w/v, 7.68%v/v and 181rpm, respectively.
27443590	4	81	theme	steep	727:731	arg1	liquor					733:738	corn steep liquor	722:738	corn steep liquor	722:738	Maximum product yield (0.12g schizophyllan/g date syrup) and schizophyllan production (8.5g/l) were obtained at concentrations of date syrup and corn steep liquor, inoculum size and agitation rate at 7.02%w/v, 0.10%w/v, 7.68%v/v and 181rpm, respectively.
27443590	2	82	used	used	300:303	arg2	method					289:294	one factor-at-a-time method	268:294	one factor-at-a-time method	268:294	Initially, one factor-at-a-time method was used to find the best carbon and nitrogen sources for schizophyllan production.
27455764	3	0	theme	polysaccharides	631:645	arg1	type					623:626	a type	621:626	a type of polysaccharides on α-TCP scaffolds	621:664	To improve mechanical properties of α-TCP porous scaffold, the present study focused on coating of a type of polysaccharides on α-TCP scaffolds.
27455764	1	1	dep	phosphate	90:98	arg1	α-Ca3					108:112	α-Ca3(PO4)2	108:118	α-Ca3(PO4)2	108:118	α-tricalcium phosphate (α-TCP, α-Ca3(PO4)2) receives great attention for bone repairing due to its biodegradability and capability of transformation to human bone's main inorganic components, hydroxyapatite (HAp).
27455764	1	1	dep	phosphate	90:98	arg1	α-TCP					101:105	α-TCP	101:105	α-TCP	101:105	α-tricalcium phosphate (α-TCP, α-Ca3(PO4)2) receives great attention for bone repairing due to its biodegradability and capability of transformation to human bone's main inorganic components, hydroxyapatite (HAp).
27455764	5	2	with	scaffolds	912:920	arg1	porosity					927:934	porosity	927:934	porosity of 75 vol%	927:945	After coating of gellan gum on α-TCP scaffolds with porosity of 75 vol%, the compressive strength increased from 0.45 MPa to around 2.00 MPa.
27455764	1	3	theme	great	130:134	arg1	attention					136:144	great attention	130:144	great attention for bone repairing due to its biodegradability and capability of transformation to human bone's main inorganic components, hydroxyapatite (HAp)	130:288	α-tricalcium phosphate (α-TCP, α-Ca3(PO4)2) receives great attention for bone repairing due to its biodegradability and capability of transformation to human bone's main inorganic components, hydroxyapatite (HAp).
27455764	1	4	theme	human	229:233	arg1	components					257:266	human bone's main inorganic components	229:266	human bone's main inorganic components	229:266	α-tricalcium phosphate (α-TCP, α-Ca3(PO4)2) receives great attention for bone repairing due to its biodegradability and capability of transformation to human bone's main inorganic components, hydroxyapatite (HAp).
27455764	1	4	theme	human	229:233	arg1	hydroxyapatite					269:282	hydroxyapatite	269:282	hydroxyapatite (HAp)	269:288	α-tricalcium phosphate (α-TCP, α-Ca3(PO4)2) receives great attention for bone repairing due to its biodegradability and capability of transformation to human bone's main inorganic components, hydroxyapatite (HAp).
27455764	3	5	theme	type	623:626	arg1	coating					610:616	coating	610:616	coating of a type of polysaccharides on α-TCP scaffolds	610:664	To improve mechanical properties of α-TCP porous scaffold, the present study focused on coating of a type of polysaccharides on α-TCP scaffolds.
27455764	7	6	theme	tissue	1284:1289	arg1	repairing					1291:1299	tissue repairing	1284:1299	tissue repairing	1284:1299	Improvement of mechanical properties of α-TCP/gellan gum composites was achieved to show easy handling performance for a bone substitute for tissue repairing.
27455764	8	7	theme	solutions	1403:1411	arg1	concentration					1383:1395	the concentration	1379:1395	the concentration of GG solutions	1379:1411	The dissolving rate of the coated scaffolds was also controlled by adjusting the concentration of GG solutions.
27455764	4	8	theme	HAp	804:806	arg1	deposition					808:817	HAp deposition	804:817	HAp deposition during hydration of α-TCP after exposure to body fluid	804:872	Gellan gum was chosen as the polysaccharide for coating because of its biodegradability as well as the potential acting as substrate for HAp deposition during hydration of α-TCP after exposure to body fluid.
27455764	6	9	theme	%	1140:1140	arg1	porosity					1122:1129	porosity	1122:1129	porosity of 63 vol%	1122:1140	Among the coated scaffold, the maximum compressive strength, 3.97 MPa, was obtained on the scaffold with porosity of 63 vol%.
27455764	5	10	theme	gellan	892:897	arg1	gum					899:901	gellan gum	892:901	gellan gum	892:901	After coating of gellan gum on α-TCP scaffolds with porosity of 75 vol%, the compressive strength increased from 0.45 MPa to around 2.00 MPa.
27455764	8	11	theme	GG	1400:1401	arg1	solutions					1403:1411	GG solutions	1400:1411	GG solutions	1400:1411	The dissolving rate of the coated scaffolds was also controlled by adjusting the concentration of GG solutions.
27455764	6	12	theme	vol	1137:1139	arg1	%					1140:1140	63 vol%	1134:1140	63 vol%	1134:1140	Among the coated scaffold, the maximum compressive strength, 3.97 MPa, was obtained on the scaffold with porosity of 63 vol%.
27455764	7	13	theme	α-TCP/gellan	1183:1194	arg1	composites					1200:1209	α-TCP/gellan gum composites	1183:1209	α-TCP/gellan gum composites	1183:1209	Improvement of mechanical properties of α-TCP/gellan gum composites was achieved to show easy handling performance for a bone substitute for tissue repairing.
27455764	5	14	theme	gum	899:901	arg1	coating					881:887	coating	881:887	coating of gellan gum on α-TCP scaffolds with porosity of 75 vol%	881:945	After coating of gellan gum on α-TCP scaffolds with porosity of 75 vol%, the compressive strength increased from 0.45 MPa to around 2.00 MPa.
27455764	2	15	theme	low-temperature	354:368	arg1	β-TCR					388:392	β-TCR	388:392	β-TCR	388:392	α-TCP porous scaffold is easily procurable by sintering of the low-temperature polymorph of TCP, β-TCR Still, porous body of α-TCP is too brittle to being handled and shaped, limiting its clinical application as implant materials.
27455764	2	15	theme	low-temperature	354:368	arg1	polymorph					370:378	the low-temperature polymorph	350:378	the low-temperature polymorph of TCP	350:385	α-TCP porous scaffold is easily procurable by sintering of the low-temperature polymorph of TCP, β-TCR Still, porous body of α-TCP is too brittle to being handled and shaped, limiting its clinical application as implant materials.
27455764	1	16	theme	α-tricalcium	77:88	arg1	phosphate					90:98	α-tricalcium phosphate	77:98	α-tricalcium phosphate (α-TCP, α-Ca3(PO4)2)	77:119	α-tricalcium phosphate (α-TCP, α-Ca3(PO4)2) receives great attention for bone repairing due to its biodegradability and capability of transformation to human bone's main inorganic components, hydroxyapatite (HAp).
27455764	2	17	theme	porous	401:406	arg1	body					408:411	porous body	401:411	porous body of α-TCP	401:420	α-TCP porous scaffold is easily procurable by sintering of the low-temperature polymorph of TCP, β-TCR Still, porous body of α-TCP is too brittle to being handled and shaped, limiting its clinical application as implant materials.
27455764	3	18	theme	α-TCP	650:654	arg1	scaffolds					656:664	α-TCP scaffolds	650:664	α-TCP scaffolds	650:664	To improve mechanical properties of α-TCP porous scaffold, the present study focused on coating of a type of polysaccharides on α-TCP scaffolds.
27455764	6	19	theme	maximum	1048:1054	arg1	strength					1068:1075	the maximum compressive strength	1044:1075	the maximum compressive strength	1044:1075	Among the coated scaffold, the maximum compressive strength, 3.97 MPa, was obtained on the scaffold with porosity of 63 vol%.
27455764	6	19	theme	maximum	1048:1054	arg1	MPa					1083:1085	3.97 MPa	1078:1085	3.97 MPa	1078:1085	Among the coated scaffold, the maximum compressive strength, 3.97 MPa, was obtained on the scaffold with porosity of 63 vol%.
27455764	7	20	theme	gum	1196:1198	arg1	composites					1200:1209	α-TCP/gellan gum composites	1183:1209	α-TCP/gellan gum composites	1183:1209	Improvement of mechanical properties of α-TCP/gellan gum composites was achieved to show easy handling performance for a bone substitute for tissue repairing.
27455764	1	21	theme	main	242:245	arg1	components					257:266	human bone's main inorganic components	229:266	human bone's main inorganic components	229:266	α-tricalcium phosphate (α-TCP, α-Ca3(PO4)2) receives great attention for bone repairing due to its biodegradability and capability of transformation to human bone's main inorganic components, hydroxyapatite (HAp).
27455764	1	21	theme	main	242:245	arg1	hydroxyapatite					269:282	hydroxyapatite	269:282	hydroxyapatite (HAp)	269:288	α-tricalcium phosphate (α-TCP, α-Ca3(PO4)2) receives great attention for bone repairing due to its biodegradability and capability of transformation to human bone's main inorganic components, hydroxyapatite (HAp).
27455764	4	22	theme	Gellan	667:672	arg1	polysaccharide					696:709	the polysaccharide	692:709	the polysaccharide	692:709	Gellan gum was chosen as the polysaccharide for coating because of its biodegradability as well as the potential acting as substrate for HAp deposition during hydration of α-TCP after exposure to body fluid.
27455764	4	22	theme	Gellan	667:672	arg1	gum					674:676	Gellan gum	667:676	Gellan gum	667:676	Gellan gum was chosen as the polysaccharide for coating because of its biodegradability as well as the potential acting as substrate for HAp deposition during hydration of α-TCP after exposure to body fluid.
27455764	7	23	theme	handling	1237:1244	arg1	performance					1246:1256	easy handling performance	1232:1256	easy handling performance	1232:1256	Improvement of mechanical properties of α-TCP/gellan gum composites was achieved to show easy handling performance for a bone substitute for tissue repairing.
27455764	7	24	theme	composites	1200:1209	arg1	properties					1169:1178	mechanical properties	1158:1178	mechanical properties of α-TCP/gellan gum composites	1158:1209	Improvement of mechanical properties of α-TCP/gellan gum composites was achieved to show easy handling performance for a bone substitute for tissue repairing.
27455764	3	25	from	scaffolds	656:664	arg1	type					623:626	a type	621:626	a type of polysaccharides on α-TCP scaffolds	621:664	To improve mechanical properties of α-TCP porous scaffold, the present study focused on coating of a type of polysaccharides on α-TCP scaffolds.
27455764	1	26	theme	inorganic	247:255	arg1	components					257:266	human bone's main inorganic components	229:266	human bone's main inorganic components	229:266	α-tricalcium phosphate (α-TCP, α-Ca3(PO4)2) receives great attention for bone repairing due to its biodegradability and capability of transformation to human bone's main inorganic components, hydroxyapatite (HAp).
27455764	1	26	theme	inorganic	247:255	arg1	hydroxyapatite					269:282	hydroxyapatite	269:282	hydroxyapatite (HAp)	269:288	α-tricalcium phosphate (α-TCP, α-Ca3(PO4)2) receives great attention for bone repairing due to its biodegradability and capability of transformation to human bone's main inorganic components, hydroxyapatite (HAp).
27455764	0	27	theme	Porous	15:20	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of Porous	0:20	Fabrication of Porous α-TCP/Gellan Gum Scaffold for Bone Tissue Engineering.
27455764	2	28	theme	clinical	479:486	arg1	application					488:498	its clinical application	475:498	its clinical application	475:498	α-TCP porous scaffold is easily procurable by sintering of the low-temperature polymorph of TCP, β-TCR Still, porous body of α-TCP is too brittle to being handled and shaped, limiting its clinical application as implant materials.
27455764	2	28	theme	clinical	479:486	arg1	materials					511:519	implant materials	503:519	implant materials	503:519	α-TCP porous scaffold is easily procurable by sintering of the low-temperature polymorph of TCP, β-TCR Still, porous body of α-TCP is too brittle to being handled and shaped, limiting its clinical application as implant materials.
27455764	3	29	theme	present	585:591	arg1	study					593:597	the present study	581:597	the present study	581:597	To improve mechanical properties of α-TCP porous scaffold, the present study focused on coating of a type of polysaccharides on α-TCP scaffolds.
27455764	2	30	theme	TCP	383:385	arg1	β-TCR					388:392	β-TCR	388:392	β-TCR	388:392	α-TCP porous scaffold is easily procurable by sintering of the low-temperature polymorph of TCP, β-TCR Still, porous body of α-TCP is too brittle to being handled and shaped, limiting its clinical application as implant materials.
27455764	2	30	theme	TCP	383:385	arg1	polymorph					370:378	the low-temperature polymorph	350:378	the low-temperature polymorph of TCP	350:385	α-TCP porous scaffold is easily procurable by sintering of the low-temperature polymorph of TCP, β-TCR Still, porous body of α-TCP is too brittle to being handled and shaped, limiting its clinical application as implant materials.
27455764	4	31	theme	body	863:866	arg1	fluid					868:872	body fluid	863:872	body fluid	863:872	Gellan gum was chosen as the polysaccharide for coating because of its biodegradability as well as the potential acting as substrate for HAp deposition during hydration of α-TCP after exposure to body fluid.
27455764	7	32	theme	mechanical	1158:1167	arg1	properties					1169:1178	mechanical properties	1158:1178	mechanical properties of α-TCP/gellan gum composites	1158:1209	Improvement of mechanical properties of α-TCP/gellan gum composites was achieved to show easy handling performance for a bone substitute for tissue repairing.
27455764	3	33	theme	mechanical	533:542	arg1	properties					544:553	mechanical properties	533:553	mechanical properties of α-TCP porous scaffold	533:578	To improve mechanical properties of α-TCP porous scaffold, the present study focused on coating of a type of polysaccharides on α-TCP scaffolds.
27455764	7	34	dep	bone	1264:1267	arg1	substitute					1269:1278	substitute	1269:1278	substitute for tissue repairing	1269:1299	Improvement of mechanical properties of α-TCP/gellan gum composites was achieved to show easy handling performance for a bone substitute for tissue repairing.
27455764	6	35	with	scaffold	1108:1115	arg1	porosity					1122:1129	porosity	1122:1129	porosity of 63 vol%	1122:1140	Among the coated scaffold, the maximum compressive strength, 3.97 MPa, was obtained on the scaffold with porosity of 63 vol%.
27455764	2	36	theme	α-TCP	416:420	arg1	body					408:411	porous body	401:411	porous body of α-TCP	401:420	α-TCP porous scaffold is easily procurable by sintering of the low-temperature polymorph of TCP, β-TCR Still, porous body of α-TCP is too brittle to being handled and shaped, limiting its clinical application as implant materials.
27455764	3	37	from	type	623:626	arg1	scaffolds					656:664	α-TCP scaffolds	650:664	α-TCP scaffolds	650:664	To improve mechanical properties of α-TCP porous scaffold, the present study focused on coating of a type of polysaccharides on α-TCP scaffolds.
27455764	0	38	theme	Gum	35:37	arg1	Scaffold					39:46	Gum Scaffold	35:46	Gum Scaffold for Bone Tissue Engineering	35:74	Fabrication of Porous α-TCP/Gellan Gum Scaffold for Bone Tissue Engineering.
27455764	8	39	theme	scaffolds	1336:1344	arg1	rate					1317:1320	The dissolving rate	1302:1320	The dissolving rate of the coated scaffolds	1302:1344	The dissolving rate of the coated scaffolds was also controlled by adjusting the concentration of GG solutions.
27455764	2	40	theme	polymorph	370:378	arg1	sintering					337:345	sintering	337:345	sintering of the low-temperature polymorph of TCP, β-TCR	337:392	α-TCP porous scaffold is easily procurable by sintering of the low-temperature polymorph of TCP, β-TCR Still, porous body of α-TCP is too brittle to being handled and shaped, limiting its clinical application as implant materials.
27455764	0	41	theme	Bone	52:55	arg1	Engineering					64:74	Bone Tissue Engineering	52:74	Bone Tissue Engineering	52:74	Fabrication of Porous α-TCP/Gellan Gum Scaffold for Bone Tissue Engineering.
27455764	8	42	theme	coated	1329:1334	arg1	scaffolds					1336:1344	the coated scaffolds	1325:1344	the coated scaffolds	1325:1344	The dissolving rate of the coated scaffolds was also controlled by adjusting the concentration of GG solutions.
27455764	5	43	theme	vol	942:944	arg1	%					945:945	75 vol%	939:945	75 vol%	939:945	After coating of gellan gum on α-TCP scaffolds with porosity of 75 vol%, the compressive strength increased from 0.45 MPa to around 2.00 MPa.
27455764	4	44	theme	α-TCP	839:843	arg1	hydration					826:834	hydration	826:834	hydration of α-TCP after exposure to body fluid	826:872	Gellan gum was chosen as the polysaccharide for coating because of its biodegradability as well as the potential acting as substrate for HAp deposition during hydration of α-TCP after exposure to body fluid.
27455764	6	45	theme	coated	1027:1032	arg1	scaffold					1034:1041	the coated scaffold	1023:1041	the coated scaffold	1023:1041	Among the coated scaffold, the maximum compressive strength, 3.97 MPa, was obtained on the scaffold with porosity of 63 vol%.
27455764	3	46	from	polysaccharides	631:645	arg1	scaffolds					656:664	α-TCP scaffolds	650:664	α-TCP scaffolds	650:664	To improve mechanical properties of α-TCP porous scaffold, the present study focused on coating of a type of polysaccharides on α-TCP scaffolds.
27455764	5	47	theme	%	945:945	arg1	porosity					927:934	porosity	927:934	porosity of 75 vol%	927:945	After coating of gellan gum on α-TCP scaffolds with porosity of 75 vol%, the compressive strength increased from 0.45 MPa to around 2.00 MPa.
27455764	2	48	theme	porous	297:302	arg1	scaffold					304:311	α-TCP porous scaffold	291:311	α-TCP porous scaffold	291:311	α-TCP porous scaffold is easily procurable by sintering of the low-temperature polymorph of TCP, β-TCR Still, porous body of α-TCP is too brittle to being handled and shaped, limiting its clinical application as implant materials.
27455764	8	49	theme	dissolving	1306:1315	arg1	rate					1317:1320	The dissolving rate	1302:1320	The dissolving rate of the coated scaffolds	1302:1344	The dissolving rate of the coated scaffolds was also controlled by adjusting the concentration of GG solutions.
27455764	2	50	theme	implant	503:509	arg1	application					488:498	its clinical application	475:498	its clinical application	475:498	α-TCP porous scaffold is easily procurable by sintering of the low-temperature polymorph of TCP, β-TCR Still, porous body of α-TCP is too brittle to being handled and shaped, limiting its clinical application as implant materials.
27455764	2	50	theme	implant	503:509	arg1	materials					511:519	implant materials	503:519	implant materials	503:519	α-TCP porous scaffold is easily procurable by sintering of the low-temperature polymorph of TCP, β-TCR Still, porous body of α-TCP is too brittle to being handled and shaped, limiting its clinical application as implant materials.
27455764	5	51	theme	α-TCP	906:910	arg1	scaffolds					912:920	α-TCP scaffolds	906:920	α-TCP scaffolds with porosity of 75 vol%	906:945	After coating of gellan gum on α-TCP scaffolds with porosity of 75 vol%, the compressive strength increased from 0.45 MPa to around 2.00 MPa.
27455764	3	52	theme	porous	564:569	arg1	scaffold					571:578	α-TCP porous scaffold	558:578	α-TCP porous scaffold	558:578	To improve mechanical properties of α-TCP porous scaffold, the present study focused on coating of a type of polysaccharides on α-TCP scaffolds.
27455764	2	53	theme	α-TCP	291:295	arg1	scaffold					304:311	α-TCP porous scaffold	291:311	α-TCP porous scaffold	291:311	α-TCP porous scaffold is easily procurable by sintering of the low-temperature polymorph of TCP, β-TCR Still, porous body of α-TCP is too brittle to being handled and shaped, limiting its clinical application as implant materials.
27455764	5	54	from	coating	881:887	arg1	scaffolds					912:920	α-TCP scaffolds	906:920	α-TCP scaffolds with porosity of 75 vol%	906:945	After coating of gellan gum on α-TCP scaffolds with porosity of 75 vol%, the compressive strength increased from 0.45 MPa to around 2.00 MPa.
27455764	6	55	theme	compressive	1056:1066	arg1	strength					1068:1075	the maximum compressive strength	1044:1075	the maximum compressive strength	1044:1075	Among the coated scaffold, the maximum compressive strength, 3.97 MPa, was obtained on the scaffold with porosity of 63 vol%.
27455764	6	55	theme	compressive	1056:1066	arg1	MPa					1083:1085	3.97 MPa	1078:1085	3.97 MPa	1078:1085	Among the coated scaffold, the maximum compressive strength, 3.97 MPa, was obtained on the scaffold with porosity of 63 vol%.
27455764	5	56	theme	compressive	952:962	arg1	strength					964:971	the compressive strength	948:971	the compressive strength	948:971	After coating of gellan gum on α-TCP scaffolds with porosity of 75 vol%, the compressive strength increased from 0.45 MPa to around 2.00 MPa.
27455764	3	57	theme	scaffold	571:578	arg1	properties					544:553	mechanical properties	533:553	mechanical properties of α-TCP porous scaffold	533:578	To improve mechanical properties of α-TCP porous scaffold, the present study focused on coating of a type of polysaccharides on α-TCP scaffolds.
27455764	7	58	theme	properties	1169:1178	arg1	Improvement					1143:1153	Improvement	1143:1153	Improvement of mechanical properties of α-TCP/gellan gum composites	1143:1209	Improvement of mechanical properties of α-TCP/gellan gum composites was achieved to show easy handling performance for a bone substitute for tissue repairing.
27455764	0	59	theme	Tissue	57:62	arg1	Engineering					64:74	Bone Tissue Engineering	52:74	Bone Tissue Engineering	52:74	Fabrication of Porous α-TCP/Gellan Gum Scaffold for Bone Tissue Engineering.
27455764	7	60	theme	easy	1232:1235	arg1	performance					1246:1256	easy handling performance	1232:1256	easy handling performance	1232:1256	Improvement of mechanical properties of α-TCP/gellan gum composites was achieved to show easy handling performance for a bone substitute for tissue repairing.
27455764	3	61	theme	α-TCP	558:562	arg1	scaffold					571:578	α-TCP porous scaffold	558:578	α-TCP porous scaffold	558:578	To improve mechanical properties of α-TCP porous scaffold, the present study focused on coating of a type of polysaccharides on α-TCP scaffolds.
27455764	1	62	theme	transformation	211:224	arg1	capability					197:206	capability	197:206	capability	197:206	α-tricalcium phosphate (α-TCP, α-Ca3(PO4)2) receives great attention for bone repairing due to its biodegradability and capability of transformation to human bone's main inorganic components, hydroxyapatite (HAp).
27455764	1	62	theme	transformation	211:224	arg1	biodegradability					176:191	biodegradability	176:191	biodegradability	176:191	α-tricalcium phosphate (α-TCP, α-Ca3(PO4)2) receives great attention for bone repairing due to its biodegradability and capability of transformation to human bone's main inorganic components, hydroxyapatite (HAp).
28782615	7	0	theme	essential	1269:1277	arg1	%					1297:1297	40.36%	1292:1297	40.36%	1292:1297	Analysis of the amino acid composition of CBGs showed a high level of essential amino acids (40.36%), in which threonine was the most relevant (10.28%).
28782615	7	0	theme	essential	1269:1277	arg1	acids					1285:1289	essential amino acids	1269:1289	essential amino acids (40.36%)	1269:1298	Analysis of the amino acid composition of CBGs showed a high level of essential amino acids (40.36%), in which threonine was the most relevant (10.28%).
28782615	5	1	theme	axial	890:894	arg1	position					896:903	the axial position	886:903	the axial position of sugar residues	886:921	Infrared spectroscopy of CBGs showed interactions between polyphenols, proteins and polysaccharides, which were characterized by α-type glycosidic bond and sulfate groups in the axial position of sugar residues.
28782615	8	2	theme	anthraquinone	1484:1496	arg1	glycosides					1498:1507	anthraquinone glycosides	1484:1507	anthraquinone glycosides	1484:1507	LC-QTOF-MS analysis of the phenolic fraction of CBGs showed a variety of phenolic compounds including flavonoids, phlorotannins and anthraquinone glycosides.
28782615	4	3	theme	carbohydrate	599:610	arg1	fraction					612:619	The carbohydrate fraction	595:619	The carbohydrate fraction	595:619	The carbohydrate fraction was sulfated (13.81%) and conjugated with proteins (9.86%) and phenolic compounds (4.98%).
28782615	7	4	from	relevant	1333:1340	arg1	level					1260:1264	a high level	1253:1264	a high level	1253:1264	Analysis of the amino acid composition of CBGs showed a high level of essential amino acids (40.36%), in which threonine was the most relevant (10.28%).
28782615	4	5	theme	phenolic	684:691	arg1	compounds					693:701	phenolic compounds	684:701	phenolic compounds (4.98%)	684:709	The carbohydrate fraction was sulfated (13.81%) and conjugated with proteins (9.86%) and phenolic compounds (4.98%).
28782615	4	5	theme	phenolic	684:691	arg1	%					708:708	4.98%	704:708	4.98%	704:708	The carbohydrate fraction was sulfated (13.81%) and conjugated with proteins (9.86%) and phenolic compounds (4.98%).
28782615	9	6	theme	reducing	1616:1623	arg1	power					1625:1629	reducing power	1616:1629	reducing power	1616:1629	CBGs exhibited potent antioxidant activities including radical scavenging activity, chelating ability and reducing power, and displayed noticeable antibacterial and antifungal activities, which may open the way to the development of a natural biopreservation strategy based on algae.
28782615	3	7	contain	contained	468:476	arg2	amount					485:490	a high amount	478:490	a high amount of polysaccharides (49.76%) that consisted mainly of neutral sugars (47.67%) and uronic acids (2.09%)	478:592	CBGs contained a high amount of polysaccharides (49.76%) that consisted mainly of neutral sugars (47.67%) and uronic acids (2.09%).
28782615	3	7	contain	contained	468:476	arg1	CBGs					463:466	CBGs	463:466	CBGs	463:466	CBGs contained a high amount of polysaccharides (49.76%) that consisted mainly of neutral sugars (47.67%) and uronic acids (2.09%).
28782615	3	7	contain	contained	468:476	arg2	%					517:517	49.76%	512:517	49.76%	512:517	CBGs contained a high amount of polysaccharides (49.76%) that consisted mainly of neutral sugars (47.67%) and uronic acids (2.09%).
28782615	3	7	contain	contained	468:476	arg2	polysaccharides					495:509	polysaccharides	495:509	polysaccharides (49.76%)	495:518	CBGs contained a high amount of polysaccharides (49.76%) that consisted mainly of neutral sugars (47.67%) and uronic acids (2.09%).
28782615	7	8	theme	amino	1279:1283	arg1	%					1297:1297	40.36%	1292:1297	40.36%	1292:1297	Analysis of the amino acid composition of CBGs showed a high level of essential amino acids (40.36%), in which threonine was the most relevant (10.28%).
28782615	7	8	theme	amino	1279:1283	arg1	acids					1285:1289	essential amino acids	1269:1289	essential amino acids (40.36%)	1269:1298	Analysis of the amino acid composition of CBGs showed a high level of essential amino acids (40.36%), in which threonine was the most relevant (10.28%).
28782615	6	9	theme	conjugated	979:988	arg1	polysaccharides					990:1004	conjugated polysaccharides	979:1004	conjugated polysaccharides	979:1004	Neutral sugars analysis of CBGs by GC-MS revealed that conjugated polysaccharides were mainly composed of galactose (34.02%), fucose (26.25%) and mannitol (21.25%) with few amounts of other sugars such as glucose (5.78%), rhamnose (4.9%), xylose (3.22%) and mannose (2.22%).
28782615	5	10	theme	Infrared	712:719	arg1	spectroscopy					721:732	Infrared spectroscopy	712:732	Infrared spectroscopy of CBGs	712:740	Infrared spectroscopy of CBGs showed interactions between polyphenols, proteins and polysaccharides, which were characterized by α-type glycosidic bond and sulfate groups in the axial position of sugar residues.
28782615	7	11	theme	acids	1285:1289	arg1	level					1260:1264	a high level	1253:1264	a high level	1253:1264	Analysis of the amino acid composition of CBGs showed a high level of essential amino acids (40.36%), in which threonine was the most relevant (10.28%).
28782615	8	12	theme	fraction	1388:1395	arg1	analysis					1363:1370	LC-QTOF-MS analysis	1352:1370	LC-QTOF-MS analysis of the phenolic fraction of CBGs	1352:1403	LC-QTOF-MS analysis of the phenolic fraction of CBGs showed a variety of phenolic compounds including flavonoids, phlorotannins and anthraquinone glycosides.
28782615	7	13	dep	relevant	1333:1340	arg1	%					1348:1348	10.28%	1343:1348	10.28%	1343:1348	Analysis of the amino acid composition of CBGs showed a high level of essential amino acids (40.36%), in which threonine was the most relevant (10.28%).
28782615	6	14	theme	sugars	1114:1119	arg1	sugars					1114:1119	other sugars	1108:1119	other sugars such as glucose (5.78%), rhamnose (4.9%), xylose (3.22%) and mannose (2.22%)	1108:1196	Neutral sugars analysis of CBGs by GC-MS revealed that conjugated polysaccharides were mainly composed of galactose (34.02%), fucose (26.25%) and mannitol (21.25%) with few amounts of other sugars such as glucose (5.78%), rhamnose (4.9%), xylose (3.22%) and mannose (2.22%).
28782615	6	14	theme	sugars	1114:1119	arg1	mannose					1182:1188	mannose	1182:1188	mannose (2.22%)	1182:1196	Neutral sugars analysis of CBGs by GC-MS revealed that conjugated polysaccharides were mainly composed of galactose (34.02%), fucose (26.25%) and mannitol (21.25%) with few amounts of other sugars such as glucose (5.78%), rhamnose (4.9%), xylose (3.22%) and mannose (2.22%).
28782615	6	14	theme	sugars	1114:1119	arg1	rhamnose					1146:1153	rhamnose	1146:1153	rhamnose (4.9%)	1146:1160	Neutral sugars analysis of CBGs by GC-MS revealed that conjugated polysaccharides were mainly composed of galactose (34.02%), fucose (26.25%) and mannitol (21.25%) with few amounts of other sugars such as glucose (5.78%), rhamnose (4.9%), xylose (3.22%) and mannose (2.22%).
28782615	6	14	theme	sugars	1114:1119	arg1	xylose					1163:1168	xylose	1163:1168	xylose (3.22%)	1163:1176	Neutral sugars analysis of CBGs by GC-MS revealed that conjugated polysaccharides were mainly composed of galactose (34.02%), fucose (26.25%) and mannitol (21.25%) with few amounts of other sugars such as glucose (5.78%), rhamnose (4.9%), xylose (3.22%) and mannose (2.22%).
28782615	6	14	theme	sugars	1114:1119	arg1	amounts					1097:1103	few amounts	1093:1103	few amounts of other sugars such as glucose (5.78%), rhamnose (4.9%), xylose (3.22%) and mannose (2.22%)	1093:1196	Neutral sugars analysis of CBGs by GC-MS revealed that conjugated polysaccharides were mainly composed of galactose (34.02%), fucose (26.25%) and mannitol (21.25%) with few amounts of other sugars such as glucose (5.78%), rhamnose (4.9%), xylose (3.22%) and mannose (2.22%).
28782615	6	14	theme	sugars	1114:1119	arg1	glucose					1129:1135	glucose	1129:1135	glucose (5.78%)	1129:1143	Neutral sugars analysis of CBGs by GC-MS revealed that conjugated polysaccharides were mainly composed of galactose (34.02%), fucose (26.25%) and mannitol (21.25%) with few amounts of other sugars such as glucose (5.78%), rhamnose (4.9%), xylose (3.22%) and mannose (2.22%).
28782615	5	15	theme	sulfate	868:874	arg1	groups					876:881	sulfate groups	868:881	sulfate groups in the axial position of sugar residues	868:921	Infrared spectroscopy of CBGs showed interactions between polyphenols, proteins and polysaccharides, which were characterized by α-type glycosidic bond and sulfate groups in the axial position of sugar residues.
28782615	1	16	theme	antioxidant	214:224	arg1	activities					244:253	the antioxidant and antimicrobial activities	210:253	the antioxidant and antimicrobial activities of naturally occurring Cystoseira barbata seaweed glyco-conjugates (CBGs)	210:327	The present study investigated the antioxidant and antimicrobial activities of naturally occurring Cystoseira barbata seaweed glyco-conjugates (CBGs), with a view to developing safer food preservatives.
28782615	8	17	theme	compounds	1434:1442	arg1	phlorotannins					1466:1478	phlorotannins	1466:1478	phlorotannins	1466:1478	LC-QTOF-MS analysis of the phenolic fraction of CBGs showed a variety of phenolic compounds including flavonoids, phlorotannins and anthraquinone glycosides.
28782615	8	17	theme	compounds	1434:1442	arg1	glycosides					1498:1507	anthraquinone glycosides	1484:1507	anthraquinone glycosides	1484:1507	LC-QTOF-MS analysis of the phenolic fraction of CBGs showed a variety of phenolic compounds including flavonoids, phlorotannins and anthraquinone glycosides.
28782615	8	17	theme	compounds	1434:1442	arg1	flavonoids					1454:1463	flavonoids	1454:1463	flavonoids	1454:1463	LC-QTOF-MS analysis of the phenolic fraction of CBGs showed a variety of phenolic compounds including flavonoids, phlorotannins and anthraquinone glycosides.
28782615	8	17	theme	compounds	1434:1442	arg1	variety					1414:1420	a variety	1412:1420	a variety of phenolic compounds including flavonoids, phlorotannins and anthraquinone glycosides	1412:1507	LC-QTOF-MS analysis of the phenolic fraction of CBGs showed a variety of phenolic compounds including flavonoids, phlorotannins and anthraquinone glycosides.
28782615	8	17	theme	compounds	1434:1442	arg1	compounds					1434:1442	phenolic compounds	1425:1442	phenolic compounds	1425:1442	LC-QTOF-MS analysis of the phenolic fraction of CBGs showed a variety of phenolic compounds including flavonoids, phlorotannins and anthraquinone glycosides.
28782615	6	18	with	mannitol	1070:1077	arg1	sugars					1114:1119	other sugars	1108:1119	other sugars such as glucose (5.78%), rhamnose (4.9%), xylose (3.22%) and mannose (2.22%)	1108:1196	Neutral sugars analysis of CBGs by GC-MS revealed that conjugated polysaccharides were mainly composed of galactose (34.02%), fucose (26.25%) and mannitol (21.25%) with few amounts of other sugars such as glucose (5.78%), rhamnose (4.9%), xylose (3.22%) and mannose (2.22%).
28782615	6	18	with	mannitol	1070:1077	arg1	mannose					1182:1188	mannose	1182:1188	mannose (2.22%)	1182:1196	Neutral sugars analysis of CBGs by GC-MS revealed that conjugated polysaccharides were mainly composed of galactose (34.02%), fucose (26.25%) and mannitol (21.25%) with few amounts of other sugars such as glucose (5.78%), rhamnose (4.9%), xylose (3.22%) and mannose (2.22%).
28782615	6	18	with	mannitol	1070:1077	arg1	rhamnose					1146:1153	rhamnose	1146:1153	rhamnose (4.9%)	1146:1160	Neutral sugars analysis of CBGs by GC-MS revealed that conjugated polysaccharides were mainly composed of galactose (34.02%), fucose (26.25%) and mannitol (21.25%) with few amounts of other sugars such as glucose (5.78%), rhamnose (4.9%), xylose (3.22%) and mannose (2.22%).
28782615	6	18	with	mannitol	1070:1077	arg1	xylose					1163:1168	xylose	1163:1168	xylose (3.22%)	1163:1176	Neutral sugars analysis of CBGs by GC-MS revealed that conjugated polysaccharides were mainly composed of galactose (34.02%), fucose (26.25%) and mannitol (21.25%) with few amounts of other sugars such as glucose (5.78%), rhamnose (4.9%), xylose (3.22%) and mannose (2.22%).
28782615	6	18	with	mannitol	1070:1077	arg1	amounts					1097:1103	few amounts	1093:1103	few amounts of other sugars such as glucose (5.78%), rhamnose (4.9%), xylose (3.22%) and mannose (2.22%)	1093:1196	Neutral sugars analysis of CBGs by GC-MS revealed that conjugated polysaccharides were mainly composed of galactose (34.02%), fucose (26.25%) and mannitol (21.25%) with few amounts of other sugars such as glucose (5.78%), rhamnose (4.9%), xylose (3.22%) and mannose (2.22%).
28782615	6	18	with	mannitol	1070:1077	arg1	glucose					1129:1135	glucose	1129:1135	glucose (5.78%)	1129:1143	Neutral sugars analysis of CBGs by GC-MS revealed that conjugated polysaccharides were mainly composed of galactose (34.02%), fucose (26.25%) and mannitol (21.25%) with few amounts of other sugars such as glucose (5.78%), rhamnose (4.9%), xylose (3.22%) and mannose (2.22%).
28782615	8	19	theme	phenolic	1425:1432	arg1	compounds					1434:1442	phenolic compounds	1425:1442	phenolic compounds	1425:1442	LC-QTOF-MS analysis of the phenolic fraction of CBGs showed a variety of phenolic compounds including flavonoids, phlorotannins and anthraquinone glycosides.
28782615	3	20	theme	uronic	573:578	arg1	acids					580:584	uronic acids	573:584	uronic acids (2.09%)	573:592	CBGs contained a high amount of polysaccharides (49.76%) that consisted mainly of neutral sugars (47.67%) and uronic acids (2.09%).
28782615	3	20	theme	uronic	573:578	arg1	%					591:591	2.09%	587:591	2.09%	587:591	CBGs contained a high amount of polysaccharides (49.76%) that consisted mainly of neutral sugars (47.67%) and uronic acids (2.09%).
28782615	9	21	theme	biopreservation	1753:1767	arg1	strategy					1769:1776	a natural biopreservation strategy	1743:1776	a natural biopreservation strategy based on algae	1743:1791	CBGs exhibited potent antioxidant activities including radical scavenging activity, chelating ability and reducing power, and displayed noticeable antibacterial and antifungal activities, which may open the way to the development of a natural biopreservation strategy based on algae.
28782615	0	22	theme	ternary	36:42	arg1	conjugates					44:53	Polyphenolic-protein-polysaccharide ternary conjugates	0:53	Polyphenolic-protein-polysaccharide ternary conjugates from Cystoseira barbata Tunisian	0:86	Polyphenolic-protein-polysaccharide ternary conjugates from Cystoseira barbata Tunisian seaweed as potential biopreservatives: Chemical, antioxidant and antimicrobial properties.
28782615	0	23	from	Cystoseira	60:69	arg1	conjugates					44:53	Polyphenolic-protein-polysaccharide ternary conjugates	0:53	Polyphenolic-protein-polysaccharide ternary conjugates from Cystoseira barbata Tunisian	0:86	Polyphenolic-protein-polysaccharide ternary conjugates from Cystoseira barbata Tunisian seaweed as potential biopreservatives: Chemical, antioxidant and antimicrobial properties.
28782615	5	24	theme	CBGs	737:740	arg1	spectroscopy					721:732	Infrared spectroscopy	712:732	Infrared spectroscopy of CBGs	712:740	Infrared spectroscopy of CBGs showed interactions between polyphenols, proteins and polysaccharides, which were characterized by α-type glycosidic bond and sulfate groups in the axial position of sugar residues.
28782615	7	25	theme	high	1255:1258	arg1	level					1260:1264	a high level	1253:1264	a high level	1253:1264	Analysis of the amino acid composition of CBGs showed a high level of essential amino acids (40.36%), in which threonine was the most relevant (10.28%).
28782615	9	26	theme	strategy	1769:1776	arg1	development					1728:1738	the development	1724:1738	the development of a natural biopreservation strategy based on algae	1724:1791	CBGs exhibited potent antioxidant activities including radical scavenging activity, chelating ability and reducing power, and displayed noticeable antibacterial and antifungal activities, which may open the way to the development of a natural biopreservation strategy based on algae.
28782615	1	27	theme	antimicrobial	230:242	arg1	activities					244:253	the antioxidant and antimicrobial activities	210:253	the antioxidant and antimicrobial activities of naturally occurring Cystoseira barbata seaweed glyco-conjugates (CBGs)	210:327	The present study investigated the antioxidant and antimicrobial activities of naturally occurring Cystoseira barbata seaweed glyco-conjugates (CBGs), with a view to developing safer food preservatives.
28782615	0	28	theme	Polyphenolic-protein-polysaccharide	0:34	arg1	conjugates					44:53	Polyphenolic-protein-polysaccharide ternary conjugates	0:53	Polyphenolic-protein-polysaccharide ternary conjugates from Cystoseira barbata Tunisian	0:86	Polyphenolic-protein-polysaccharide ternary conjugates from Cystoseira barbata Tunisian seaweed as potential biopreservatives: Chemical, antioxidant and antimicrobial properties.
28782615	8	29	theme	LC-QTOF-MS	1352:1361	arg1	analysis					1363:1370	LC-QTOF-MS analysis	1352:1370	LC-QTOF-MS analysis of the phenolic fraction of CBGs	1352:1403	LC-QTOF-MS analysis of the phenolic fraction of CBGs showed a variety of phenolic compounds including flavonoids, phlorotannins and anthraquinone glycosides.
28782615	0	30	dep	biopreservatives	109:124	arg1	antioxidant					137:147	antioxidant	137:147	antioxidant	137:147	Polyphenolic-protein-polysaccharide ternary conjugates from Cystoseira barbata Tunisian seaweed as potential biopreservatives: Chemical, antioxidant and antimicrobial properties.
28782615	0	30	dep	biopreservatives	109:124	arg1	properties					167:176	antimicrobial properties	153:176	antimicrobial properties	153:176	Polyphenolic-protein-polysaccharide ternary conjugates from Cystoseira barbata Tunisian seaweed as potential biopreservatives: Chemical, antioxidant and antimicrobial properties.
28782615	0	30	dep	biopreservatives	109:124	arg1	Chemical					127:134	Chemical	127:134	Chemical	127:134	Polyphenolic-protein-polysaccharide ternary conjugates from Cystoseira barbata Tunisian seaweed as potential biopreservatives: Chemical, antioxidant and antimicrobial properties.
28782615	3	31	theme	polysaccharides	495:509	arg1	polysaccharides					495:509	polysaccharides	495:509	polysaccharides (49.76%)	495:518	CBGs contained a high amount of polysaccharides (49.76%) that consisted mainly of neutral sugars (47.67%) and uronic acids (2.09%).
28782615	3	31	theme	polysaccharides	495:509	arg1	%					517:517	49.76%	512:517	49.76%	512:517	CBGs contained a high amount of polysaccharides (49.76%) that consisted mainly of neutral sugars (47.67%) and uronic acids (2.09%).
28782615	3	31	theme	polysaccharides	495:509	arg1	amount					485:490	a high amount	478:490	a high amount of polysaccharides (49.76%) that consisted mainly of neutral sugars (47.67%) and uronic acids (2.09%)	478:592	CBGs contained a high amount of polysaccharides (49.76%) that consisted mainly of neutral sugars (47.67%) and uronic acids (2.09%).
28782615	7	32	theme	acid	1221:1224	arg1	composition					1226:1236	the amino acid composition	1211:1236	the amino acid composition of CBGs	1211:1244	Analysis of the amino acid composition of CBGs showed a high level of essential amino acids (40.36%), in which threonine was the most relevant (10.28%).
28782615	2	33	dep	isolated	405:412	arg1	characterized					448:460	characterized	448:460	characterized	448:460	CBGs were successfully isolated, then chemically and structurally characterized.
28782615	0	34	theme	antimicrobial	153:165	arg1	properties					167:176	antimicrobial properties	153:176	antimicrobial properties	153:176	Polyphenolic-protein-polysaccharide ternary conjugates from Cystoseira barbata Tunisian seaweed as potential biopreservatives: Chemical, antioxidant and antimicrobial properties.
28782615	6	35	with	fucose	1050:1055	arg1	sugars					1114:1119	other sugars	1108:1119	other sugars such as glucose (5.78%), rhamnose (4.9%), xylose (3.22%) and mannose (2.22%)	1108:1196	Neutral sugars analysis of CBGs by GC-MS revealed that conjugated polysaccharides were mainly composed of galactose (34.02%), fucose (26.25%) and mannitol (21.25%) with few amounts of other sugars such as glucose (5.78%), rhamnose (4.9%), xylose (3.22%) and mannose (2.22%).
28782615	6	35	with	fucose	1050:1055	arg1	mannose					1182:1188	mannose	1182:1188	mannose (2.22%)	1182:1196	Neutral sugars analysis of CBGs by GC-MS revealed that conjugated polysaccharides were mainly composed of galactose (34.02%), fucose (26.25%) and mannitol (21.25%) with few amounts of other sugars such as glucose (5.78%), rhamnose (4.9%), xylose (3.22%) and mannose (2.22%).
28782615	6	35	with	fucose	1050:1055	arg1	rhamnose					1146:1153	rhamnose	1146:1153	rhamnose (4.9%)	1146:1160	Neutral sugars analysis of CBGs by GC-MS revealed that conjugated polysaccharides were mainly composed of galactose (34.02%), fucose (26.25%) and mannitol (21.25%) with few amounts of other sugars such as glucose (5.78%), rhamnose (4.9%), xylose (3.22%) and mannose (2.22%).
28782615	6	35	with	fucose	1050:1055	arg1	xylose					1163:1168	xylose	1163:1168	xylose (3.22%)	1163:1176	Neutral sugars analysis of CBGs by GC-MS revealed that conjugated polysaccharides were mainly composed of galactose (34.02%), fucose (26.25%) and mannitol (21.25%) with few amounts of other sugars such as glucose (5.78%), rhamnose (4.9%), xylose (3.22%) and mannose (2.22%).
28782615	6	35	with	fucose	1050:1055	arg1	amounts					1097:1103	few amounts	1093:1103	few amounts of other sugars such as glucose (5.78%), rhamnose (4.9%), xylose (3.22%) and mannose (2.22%)	1093:1196	Neutral sugars analysis of CBGs by GC-MS revealed that conjugated polysaccharides were mainly composed of galactose (34.02%), fucose (26.25%) and mannitol (21.25%) with few amounts of other sugars such as glucose (5.78%), rhamnose (4.9%), xylose (3.22%) and mannose (2.22%).
28782615	6	35	with	fucose	1050:1055	arg1	glucose					1129:1135	glucose	1129:1135	glucose (5.78%)	1129:1143	Neutral sugars analysis of CBGs by GC-MS revealed that conjugated polysaccharides were mainly composed of galactose (34.02%), fucose (26.25%) and mannitol (21.25%) with few amounts of other sugars such as glucose (5.78%), rhamnose (4.9%), xylose (3.22%) and mannose (2.22%).
28782615	9	36	theme	natural	1745:1751	arg1	strategy					1769:1776	a natural biopreservation strategy	1743:1776	a natural biopreservation strategy based on algae	1743:1791	CBGs exhibited potent antioxidant activities including radical scavenging activity, chelating ability and reducing power, and displayed noticeable antibacterial and antifungal activities, which may open the way to the development of a natural biopreservation strategy based on algae.
28782615	3	37	theme	high	480:483	arg1	polysaccharides					495:509	polysaccharides	495:509	polysaccharides (49.76%)	495:518	CBGs contained a high amount of polysaccharides (49.76%) that consisted mainly of neutral sugars (47.67%) and uronic acids (2.09%).
28782615	3	37	theme	high	480:483	arg1	%					517:517	49.76%	512:517	49.76%	512:517	CBGs contained a high amount of polysaccharides (49.76%) that consisted mainly of neutral sugars (47.67%) and uronic acids (2.09%).
28782615	3	37	theme	high	480:483	arg1	amount					485:490	a high amount	478:490	a high amount of polysaccharides (49.76%) that consisted mainly of neutral sugars (47.67%) and uronic acids (2.09%)	478:592	CBGs contained a high amount of polysaccharides (49.76%) that consisted mainly of neutral sugars (47.67%) and uronic acids (2.09%).
28782615	6	38	theme	CBGs	951:954	arg1	analysis					939:946	Neutral sugars analysis	924:946	Neutral sugars analysis of CBGs by GC-MS	924:963	Neutral sugars analysis of CBGs by GC-MS revealed that conjugated polysaccharides were mainly composed of galactose (34.02%), fucose (26.25%) and mannitol (21.25%) with few amounts of other sugars such as glucose (5.78%), rhamnose (4.9%), xylose (3.22%) and mannose (2.22%).
28782615	9	39	theme	antioxidant	1532:1542	arg1	ability					1604:1610	chelating ability	1594:1610	chelating ability	1594:1610	CBGs exhibited potent antioxidant activities including radical scavenging activity, chelating ability and reducing power, and displayed noticeable antibacterial and antifungal activities, which may open the way to the development of a natural biopreservation strategy based on algae.
28782615	9	39	theme	antioxidant	1532:1542	arg1	power					1625:1629	reducing power	1616:1629	reducing power	1616:1629	CBGs exhibited potent antioxidant activities including radical scavenging activity, chelating ability and reducing power, and displayed noticeable antibacterial and antifungal activities, which may open the way to the development of a natural biopreservation strategy based on algae.
28782615	9	39	theme	antioxidant	1532:1542	arg1	activities					1544:1553	potent antioxidant activities	1525:1553	potent antioxidant activities including radical scavenging activity, chelating ability and reducing power	1525:1629	CBGs exhibited potent antioxidant activities including radical scavenging activity, chelating ability and reducing power, and displayed noticeable antibacterial and antifungal activities, which may open the way to the development of a natural biopreservation strategy based on algae.
28782615	9	39	theme	antioxidant	1532:1542	arg1	activity					1584:1591	radical scavenging activity	1565:1591	radical scavenging activity	1565:1591	CBGs exhibited potent antioxidant activities including radical scavenging activity, chelating ability and reducing power, and displayed noticeable antibacterial and antifungal activities, which may open the way to the development of a natural biopreservation strategy based on algae.
28782615	5	40	theme	α-type	841:846	arg1	bond					859:862	α-type glycosidic bond	841:862	α-type glycosidic bond	841:862	Infrared spectroscopy of CBGs showed interactions between polyphenols, proteins and polysaccharides, which were characterized by α-type glycosidic bond and sulfate groups in the axial position of sugar residues.
28782615	1	41	theme	occurring	268:276	arg1	glyco-conjugates					305:320	naturally occurring Cystoseira barbata seaweed glyco-conjugates	258:320	naturally occurring Cystoseira barbata seaweed glyco-conjugates (CBGs)	258:327	The present study investigated the antioxidant and antimicrobial activities of naturally occurring Cystoseira barbata seaweed glyco-conjugates (CBGs), with a view to developing safer food preservatives.
28782615	1	41	theme	occurring	268:276	arg1	CBGs					323:326	CBGs	323:326	CBGs	323:326	The present study investigated the antioxidant and antimicrobial activities of naturally occurring Cystoseira barbata seaweed glyco-conjugates (CBGs), with a view to developing safer food preservatives.
28782615	1	42	theme	safer	356:360	arg1	preservatives					367:379	safer food preservatives	356:379	safer food preservatives	356:379	The present study investigated the antioxidant and antimicrobial activities of naturally occurring Cystoseira barbata seaweed glyco-conjugates (CBGs), with a view to developing safer food preservatives.
28782615	7	43	theme	CBGs	1241:1244	arg1	composition					1226:1236	the amino acid composition	1211:1236	the amino acid composition of CBGs	1211:1244	Analysis of the amino acid composition of CBGs showed a high level of essential amino acids (40.36%), in which threonine was the most relevant (10.28%).
28782615	5	44	theme	glycosidic	848:857	arg1	bond					859:862	α-type glycosidic bond	841:862	α-type glycosidic bond	841:862	Infrared spectroscopy of CBGs showed interactions between polyphenols, proteins and polysaccharides, which were characterized by α-type glycosidic bond and sulfate groups in the axial position of sugar residues.
28782615	1	45	theme	Cystoseira	278:287	arg1	glyco-conjugates					305:320	naturally occurring Cystoseira barbata seaweed glyco-conjugates	258:320	naturally occurring Cystoseira barbata seaweed glyco-conjugates (CBGs)	258:327	The present study investigated the antioxidant and antimicrobial activities of naturally occurring Cystoseira barbata seaweed glyco-conjugates (CBGs), with a view to developing safer food preservatives.
28782615	1	45	theme	Cystoseira	278:287	arg1	CBGs					323:326	CBGs	323:326	CBGs	323:326	The present study investigated the antioxidant and antimicrobial activities of naturally occurring Cystoseira barbata seaweed glyco-conjugates (CBGs), with a view to developing safer food preservatives.
28782615	1	46	theme	food	362:365	arg1	preservatives					367:379	safer food preservatives	356:379	safer food preservatives	356:379	The present study investigated the antioxidant and antimicrobial activities of naturally occurring Cystoseira barbata seaweed glyco-conjugates (CBGs), with a view to developing safer food preservatives.
28782615	6	47	with	galactose	1030:1038	arg1	sugars					1114:1119	other sugars	1108:1119	other sugars such as glucose (5.78%), rhamnose (4.9%), xylose (3.22%) and mannose (2.22%)	1108:1196	Neutral sugars analysis of CBGs by GC-MS revealed that conjugated polysaccharides were mainly composed of galactose (34.02%), fucose (26.25%) and mannitol (21.25%) with few amounts of other sugars such as glucose (5.78%), rhamnose (4.9%), xylose (3.22%) and mannose (2.22%).
28782615	6	47	with	galactose	1030:1038	arg1	mannose					1182:1188	mannose	1182:1188	mannose (2.22%)	1182:1196	Neutral sugars analysis of CBGs by GC-MS revealed that conjugated polysaccharides were mainly composed of galactose (34.02%), fucose (26.25%) and mannitol (21.25%) with few amounts of other sugars such as glucose (5.78%), rhamnose (4.9%), xylose (3.22%) and mannose (2.22%).
28782615	6	47	with	galactose	1030:1038	arg1	rhamnose					1146:1153	rhamnose	1146:1153	rhamnose (4.9%)	1146:1160	Neutral sugars analysis of CBGs by GC-MS revealed that conjugated polysaccharides were mainly composed of galactose (34.02%), fucose (26.25%) and mannitol (21.25%) with few amounts of other sugars such as glucose (5.78%), rhamnose (4.9%), xylose (3.22%) and mannose (2.22%).
28782615	6	47	with	galactose	1030:1038	arg1	xylose					1163:1168	xylose	1163:1168	xylose (3.22%)	1163:1176	Neutral sugars analysis of CBGs by GC-MS revealed that conjugated polysaccharides were mainly composed of galactose (34.02%), fucose (26.25%) and mannitol (21.25%) with few amounts of other sugars such as glucose (5.78%), rhamnose (4.9%), xylose (3.22%) and mannose (2.22%).
28782615	6	47	with	galactose	1030:1038	arg1	amounts					1097:1103	few amounts	1093:1103	few amounts of other sugars such as glucose (5.78%), rhamnose (4.9%), xylose (3.22%) and mannose (2.22%)	1093:1196	Neutral sugars analysis of CBGs by GC-MS revealed that conjugated polysaccharides were mainly composed of galactose (34.02%), fucose (26.25%) and mannitol (21.25%) with few amounts of other sugars such as glucose (5.78%), rhamnose (4.9%), xylose (3.22%) and mannose (2.22%).
28782615	6	47	with	galactose	1030:1038	arg1	glucose					1129:1135	glucose	1129:1135	glucose (5.78%)	1129:1143	Neutral sugars analysis of CBGs by GC-MS revealed that conjugated polysaccharides were mainly composed of galactose (34.02%), fucose (26.25%) and mannitol (21.25%) with few amounts of other sugars such as glucose (5.78%), rhamnose (4.9%), xylose (3.22%) and mannose (2.22%).
28782615	3	48	theme	neutral	545:551	arg1	sugars					553:558	neutral sugars	545:558	neutral sugars (47.67%)	545:567	CBGs contained a high amount of polysaccharides (49.76%) that consisted mainly of neutral sugars (47.67%) and uronic acids (2.09%).
28782615	3	48	theme	neutral	545:551	arg1	%					566:566	47.67%	561:566	47.67%	561:566	CBGs contained a high amount of polysaccharides (49.76%) that consisted mainly of neutral sugars (47.67%) and uronic acids (2.09%).
28782615	6	49	theme	sugars	932:937	arg1	analysis					939:946	Neutral sugars analysis	924:946	Neutral sugars analysis of CBGs by GC-MS	924:963	Neutral sugars analysis of CBGs by GC-MS revealed that conjugated polysaccharides were mainly composed of galactose (34.02%), fucose (26.25%) and mannitol (21.25%) with few amounts of other sugars such as glucose (5.78%), rhamnose (4.9%), xylose (3.22%) and mannose (2.22%).
28782615	9	50	theme	radical	1565:1571	arg1	activity					1584:1591	radical scavenging activity	1565:1591	radical scavenging activity	1565:1591	CBGs exhibited potent antioxidant activities including radical scavenging activity, chelating ability and reducing power, and displayed noticeable antibacterial and antifungal activities, which may open the way to the development of a natural biopreservation strategy based on algae.
28782615	8	51	theme	phenolic	1379:1386	arg1	fraction					1388:1395	the phenolic fraction	1375:1395	the phenolic fraction of CBGs	1375:1403	LC-QTOF-MS analysis of the phenolic fraction of CBGs showed a variety of phenolic compounds including flavonoids, phlorotannins and anthraquinone glycosides.
28782615	7	52	theme	composition	1226:1236	arg1	Analysis					1199:1206	Analysis	1199:1206	Analysis of the amino acid composition of CBGs	1199:1244	Analysis of the amino acid composition of CBGs showed a high level of essential amino acids (40.36%), in which threonine was the most relevant (10.28%).
28782615	5	53	theme	sugar	908:912	arg1	residues					914:921	sugar residues	908:921	sugar residues	908:921	Infrared spectroscopy of CBGs showed interactions between polyphenols, proteins and polysaccharides, which were characterized by α-type glycosidic bond and sulfate groups in the axial position of sugar residues.
28782615	6	54	theme	Neutral	924:930	arg1	analysis					939:946	Neutral sugars analysis	924:946	Neutral sugars analysis of CBGs by GC-MS	924:963	Neutral sugars analysis of CBGs by GC-MS revealed that conjugated polysaccharides were mainly composed of galactose (34.02%), fucose (26.25%) and mannitol (21.25%) with few amounts of other sugars such as glucose (5.78%), rhamnose (4.9%), xylose (3.22%) and mannose (2.22%).
28782615	9	55	theme	scavenging	1573:1582	arg1	activity					1584:1591	radical scavenging activity	1565:1591	radical scavenging activity	1565:1591	CBGs exhibited potent antioxidant activities including radical scavenging activity, chelating ability and reducing power, and displayed noticeable antibacterial and antifungal activities, which may open the way to the development of a natural biopreservation strategy based on algae.
28782615	5	56	theme	residues	914:921	arg1	position					896:903	the axial position	886:903	the axial position of sugar residues	886:921	Infrared spectroscopy of CBGs showed interactions between polyphenols, proteins and polysaccharides, which were characterized by α-type glycosidic bond and sulfate groups in the axial position of sugar residues.
28782615	6	57	theme	few	1093:1095	arg1	sugars					1114:1119	other sugars	1108:1119	other sugars such as glucose (5.78%), rhamnose (4.9%), xylose (3.22%) and mannose (2.22%)	1108:1196	Neutral sugars analysis of CBGs by GC-MS revealed that conjugated polysaccharides were mainly composed of galactose (34.02%), fucose (26.25%) and mannitol (21.25%) with few amounts of other sugars such as glucose (5.78%), rhamnose (4.9%), xylose (3.22%) and mannose (2.22%).
28782615	6	57	theme	few	1093:1095	arg1	mannose					1182:1188	mannose	1182:1188	mannose (2.22%)	1182:1196	Neutral sugars analysis of CBGs by GC-MS revealed that conjugated polysaccharides were mainly composed of galactose (34.02%), fucose (26.25%) and mannitol (21.25%) with few amounts of other sugars such as glucose (5.78%), rhamnose (4.9%), xylose (3.22%) and mannose (2.22%).
28782615	6	57	theme	few	1093:1095	arg1	rhamnose					1146:1153	rhamnose	1146:1153	rhamnose (4.9%)	1146:1160	Neutral sugars analysis of CBGs by GC-MS revealed that conjugated polysaccharides were mainly composed of galactose (34.02%), fucose (26.25%) and mannitol (21.25%) with few amounts of other sugars such as glucose (5.78%), rhamnose (4.9%), xylose (3.22%) and mannose (2.22%).
28782615	6	57	theme	few	1093:1095	arg1	xylose					1163:1168	xylose	1163:1168	xylose (3.22%)	1163:1176	Neutral sugars analysis of CBGs by GC-MS revealed that conjugated polysaccharides were mainly composed of galactose (34.02%), fucose (26.25%) and mannitol (21.25%) with few amounts of other sugars such as glucose (5.78%), rhamnose (4.9%), xylose (3.22%) and mannose (2.22%).
28782615	6	57	theme	few	1093:1095	arg1	amounts					1097:1103	few amounts	1093:1103	few amounts of other sugars such as glucose (5.78%), rhamnose (4.9%), xylose (3.22%) and mannose (2.22%)	1093:1196	Neutral sugars analysis of CBGs by GC-MS revealed that conjugated polysaccharides were mainly composed of galactose (34.02%), fucose (26.25%) and mannitol (21.25%) with few amounts of other sugars such as glucose (5.78%), rhamnose (4.9%), xylose (3.22%) and mannose (2.22%).
28782615	6	57	theme	few	1093:1095	arg1	glucose					1129:1135	glucose	1129:1135	glucose (5.78%)	1129:1143	Neutral sugars analysis of CBGs by GC-MS revealed that conjugated polysaccharides were mainly composed of galactose (34.02%), fucose (26.25%) and mannitol (21.25%) with few amounts of other sugars such as glucose (5.78%), rhamnose (4.9%), xylose (3.22%) and mannose (2.22%).
28782615	1	58	theme	barbata	289:295	arg1	glyco-conjugates					305:320	naturally occurring Cystoseira barbata seaweed glyco-conjugates	258:320	naturally occurring Cystoseira barbata seaweed glyco-conjugates (CBGs)	258:327	The present study investigated the antioxidant and antimicrobial activities of naturally occurring Cystoseira barbata seaweed glyco-conjugates (CBGs), with a view to developing safer food preservatives.
28782615	1	58	theme	barbata	289:295	arg1	CBGs					323:326	CBGs	323:326	CBGs	323:326	The present study investigated the antioxidant and antimicrobial activities of naturally occurring Cystoseira barbata seaweed glyco-conjugates (CBGs), with a view to developing safer food preservatives.
28782615	6	59	theme	other	1108:1112	arg1	mannose					1182:1188	mannose	1182:1188	mannose (2.22%)	1182:1196	Neutral sugars analysis of CBGs by GC-MS revealed that conjugated polysaccharides were mainly composed of galactose (34.02%), fucose (26.25%) and mannitol (21.25%) with few amounts of other sugars such as glucose (5.78%), rhamnose (4.9%), xylose (3.22%) and mannose (2.22%).
28782615	6	59	theme	other	1108:1112	arg1	sugars					1114:1119	other sugars	1108:1119	other sugars such as glucose (5.78%), rhamnose (4.9%), xylose (3.22%) and mannose (2.22%)	1108:1196	Neutral sugars analysis of CBGs by GC-MS revealed that conjugated polysaccharides were mainly composed of galactose (34.02%), fucose (26.25%) and mannitol (21.25%) with few amounts of other sugars such as glucose (5.78%), rhamnose (4.9%), xylose (3.22%) and mannose (2.22%).
28782615	6	59	theme	other	1108:1112	arg1	glucose					1129:1135	glucose	1129:1135	glucose (5.78%)	1129:1143	Neutral sugars analysis of CBGs by GC-MS revealed that conjugated polysaccharides were mainly composed of galactose (34.02%), fucose (26.25%) and mannitol (21.25%) with few amounts of other sugars such as glucose (5.78%), rhamnose (4.9%), xylose (3.22%) and mannose (2.22%).
28782615	6	59	theme	other	1108:1112	arg1	rhamnose					1146:1153	rhamnose	1146:1153	rhamnose (4.9%)	1146:1160	Neutral sugars analysis of CBGs by GC-MS revealed that conjugated polysaccharides were mainly composed of galactose (34.02%), fucose (26.25%) and mannitol (21.25%) with few amounts of other sugars such as glucose (5.78%), rhamnose (4.9%), xylose (3.22%) and mannose (2.22%).
28782615	6	59	theme	other	1108:1112	arg1	xylose					1163:1168	xylose	1163:1168	xylose (3.22%)	1163:1176	Neutral sugars analysis of CBGs by GC-MS revealed that conjugated polysaccharides were mainly composed of galactose (34.02%), fucose (26.25%) and mannitol (21.25%) with few amounts of other sugars such as glucose (5.78%), rhamnose (4.9%), xylose (3.22%) and mannose (2.22%).
28782615	7	60	theme	amino	1215:1219	arg1	composition					1226:1236	the amino acid composition	1211:1236	the amino acid composition of CBGs	1211:1244	Analysis of the amino acid composition of CBGs showed a high level of essential amino acids (40.36%), in which threonine was the most relevant (10.28%).
28782615	9	61	theme	antifungal	1675:1684	arg1	activities					1686:1695	noticeable antibacterial and antifungal activities	1646:1695	activities	1686:1695	CBGs exhibited potent antioxidant activities including radical scavenging activity, chelating ability and reducing power, and displayed noticeable antibacterial and antifungal activities, which may open the way to the development of a natural biopreservation strategy based on algae.
28782615	1	62	theme	seaweed	297:303	arg1	glyco-conjugates					305:320	naturally occurring Cystoseira barbata seaweed glyco-conjugates	258:320	naturally occurring Cystoseira barbata seaweed glyco-conjugates (CBGs)	258:327	The present study investigated the antioxidant and antimicrobial activities of naturally occurring Cystoseira barbata seaweed glyco-conjugates (CBGs), with a view to developing safer food preservatives.
28782615	1	62	theme	seaweed	297:303	arg1	CBGs					323:326	CBGs	323:326	CBGs	323:326	The present study investigated the antioxidant and antimicrobial activities of naturally occurring Cystoseira barbata seaweed glyco-conjugates (CBGs), with a view to developing safer food preservatives.
28782615	0	63	theme	potential	99:107	arg1	biopreservatives					109:124	potential biopreservatives	99:124	potential biopreservatives: Chemical, antioxidant and antimicrobial properties	99:176	Polyphenolic-protein-polysaccharide ternary conjugates from Cystoseira barbata Tunisian seaweed as potential biopreservatives: Chemical, antioxidant and antimicrobial properties.
28782615	4	64	dep	sulfated	625:632	arg1	%					640:640	13.81%	635:640	13.81%	635:640	The carbohydrate fraction was sulfated (13.81%) and conjugated with proteins (9.86%) and phenolic compounds (4.98%).
28782615	5	65	from	groups	876:881	arg1	position					896:903	the axial position	886:903	the axial position of sugar residues	886:921	Infrared spectroscopy of CBGs showed interactions between polyphenols, proteins and polysaccharides, which were characterized by α-type glycosidic bond and sulfate groups in the axial position of sugar residues.
28782615	1	66	theme	present	183:189	arg1	study					191:195	The present study	179:195	The present study	179:195	The present study investigated the antioxidant and antimicrobial activities of naturally occurring Cystoseira barbata seaweed glyco-conjugates (CBGs), with a view to developing safer food preservatives.
28782615	7	67	from	level	1260:1264	arg1	relevant					1333:1340	relevant	1333:1340	relevant	1333:1340	Analysis of the amino acid composition of CBGs showed a high level of essential amino acids (40.36%), in which threonine was the most relevant (10.28%).
28782615	7	67	from	level	1260:1264	arg1	threonine					1310:1318	threonine	1310:1318	threonine	1310:1318	Analysis of the amino acid composition of CBGs showed a high level of essential amino acids (40.36%), in which threonine was the most relevant (10.28%).
28782615	1	68	theme	glyco-conjugates	305:320	arg1	activities					244:253	the antioxidant and antimicrobial activities	210:253	the antioxidant and antimicrobial activities of naturally occurring Cystoseira barbata seaweed glyco-conjugates (CBGs)	210:327	The present study investigated the antioxidant and antimicrobial activities of naturally occurring Cystoseira barbata seaweed glyco-conjugates (CBGs), with a view to developing safer food preservatives.
28782615	9	69	theme	chelating	1594:1602	arg1	ability					1604:1610	chelating ability	1594:1610	chelating ability	1594:1610	CBGs exhibited potent antioxidant activities including radical scavenging activity, chelating ability and reducing power, and displayed noticeable antibacterial and antifungal activities, which may open the way to the development of a natural biopreservation strategy based on algae.
28782615	8	70	theme	CBGs	1400:1403	arg1	fraction					1388:1395	the phenolic fraction	1375:1395	the phenolic fraction of CBGs	1375:1403	LC-QTOF-MS analysis of the phenolic fraction of CBGs showed a variety of phenolic compounds including flavonoids, phlorotannins and anthraquinone glycosides.
28782615	5	71	from	bond	859:862	arg1	position					896:903	the axial position	886:903	the axial position of sugar residues	886:921	Infrared spectroscopy of CBGs showed interactions between polyphenols, proteins and polysaccharides, which were characterized by α-type glycosidic bond and sulfate groups in the axial position of sugar residues.
28782615	9	72	theme	potent	1525:1530	arg1	ability					1604:1610	chelating ability	1594:1610	chelating ability	1594:1610	CBGs exhibited potent antioxidant activities including radical scavenging activity, chelating ability and reducing power, and displayed noticeable antibacterial and antifungal activities, which may open the way to the development of a natural biopreservation strategy based on algae.
28782615	9	72	theme	potent	1525:1530	arg1	power					1625:1629	reducing power	1616:1629	reducing power	1616:1629	CBGs exhibited potent antioxidant activities including radical scavenging activity, chelating ability and reducing power, and displayed noticeable antibacterial and antifungal activities, which may open the way to the development of a natural biopreservation strategy based on algae.
28782615	9	72	theme	potent	1525:1530	arg1	activities					1544:1553	potent antioxidant activities	1525:1553	potent antioxidant activities including radical scavenging activity, chelating ability and reducing power	1525:1629	CBGs exhibited potent antioxidant activities including radical scavenging activity, chelating ability and reducing power, and displayed noticeable antibacterial and antifungal activities, which may open the way to the development of a natural biopreservation strategy based on algae.
28782615	9	72	theme	potent	1525:1530	arg1	activity					1584:1591	radical scavenging activity	1565:1591	radical scavenging activity	1565:1591	CBGs exhibited potent antioxidant activities including radical scavenging activity, chelating ability and reducing power, and displayed noticeable antibacterial and antifungal activities, which may open the way to the development of a natural biopreservation strategy based on algae.
26362922	5	0	theme	main	827:830	arg1	chain					832:836	the main chain	823:836	the main chain of cellulose, xylan, mannan and pectin	823:875	Although hydrolases and cellulosome-based systems predicted to be involved in degradation of the main chain of cellulose, xylan, mannan and pectin were identified, transcripts with glycoside hydrolase modules targeting the side chains of noncellulosic polysaccharides were more abundant.
26362922	7	1	theme	intestinal	1268:1277	arg1	microbiome					1279:1288	the grass carp intestinal microbiome	1253:1288	the grass carp intestinal microbiome	1253:1288	These results suggest that the grass carp intestinal microbiome functions in carbohydrate turnover and fermentation, which likely provides energy for both host and microbiota.
26362922	6	2	from	categories	1068:1077	arg1	microbiome					1097:1106	the intestinal microbiome	1082:1106	the intestinal microbiome	1082:1106	Predominant 'COG' (Clusters of Orthologous Group) categories in the intestinal microbiome included those for energy production and conversion, as well as carbohydrate and amino acid transport and metabolism.
26362922	1	3	theme	metabolic	162:170	arg1	capacity					172:179	metabolic capacity	162:179	metabolic capacity	162:179	Despite the economic importance of fish, the ecology and metabolic capacity of fish microbiomes are largely unknown.
26362922	6	4	theme	Group	1061:1065	arg1	Clusters					1037:1044	Clusters	1037:1044	Clusters of Orthologous Group	1037:1065	Predominant 'COG' (Clusters of Orthologous Group) categories in the intestinal microbiome included those for energy production and conversion, as well as carbohydrate and amino acid transport and metabolism.
26362922	5	5	theme	chain	832:836	arg1	degradation					808:818	degradation	808:818	degradation of the main chain of cellulose, xylan, mannan and pectin	808:875	Although hydrolases and cellulosome-based systems predicted to be involved in degradation of the main chain of cellulose, xylan, mannan and pectin were identified, transcripts with glycoside hydrolase modules targeting the side chains of noncellulosic polysaccharides were more abundant.
26362922	5	6	theme	side	953:956	arg1	chains					958:963	the side chains	949:963	the side chains of noncellulosic polysaccharides	949:996	Although hydrolases and cellulosome-based systems predicted to be involved in degradation of the main chain of cellulose, xylan, mannan and pectin were identified, transcripts with glycoside hydrolase modules targeting the side chains of noncellulosic polysaccharides were more abundant.
26362922	4	7	theme	fish	614:617	arg1	microbiomes					599:609	the microbiomes	595:609	the microbiomes of fish fed with different experimental diets	595:655	Comparative transcriptomes of the microbiomes of fish fed with different experimental diets indicated that the bacterial transcriptomes are influenced by host diet.
26362922	7	8	theme	grass	1257:1261	arg1	carp					1263:1266	the grass carp	1253:1266	the grass carp intestinal microbiome	1253:1288	These results suggest that the grass carp intestinal microbiome functions in carbohydrate turnover and fermentation, which likely provides energy for both host and microbiota.
26362922	2	9	theme	carp	301:304	arg1	microbiota					281:290	the intestinal microbiota	266:290	the intestinal microbiota of grass carp	266:304	Here, we sequenced the metatranscriptome of the intestinal microbiota of grass carp, Ctenopharyngodon idellus, a freshwater herbivorous fish species.
26362922	2	9	theme	carp	301:304	arg1	idellus					324:330	Ctenopharyngodon idellus	307:330	Ctenopharyngodon idellus	307:330	Here, we sequenced the metatranscriptome of the intestinal microbiota of grass carp, Ctenopharyngodon idellus, a freshwater herbivorous fish species.
26362922	2	9	theme	carp	301:304	arg1	species					363:369	a freshwater herbivorous fish species	333:369	a freshwater herbivorous fish species	333:369	Here, we sequenced the metatranscriptome of the intestinal microbiota of grass carp, Ctenopharyngodon idellus, a freshwater herbivorous fish species.
26362922	8	10	theme	evolutionary	1453:1464	arg1	adaption					1466:1473	its evolutionary adaption	1449:1473	its evolutionary adaption for harvesting nutrients for an herbivore with a high-throughput nutritional strategy that is not dominated by cellulose digestion but rather the degradation of intracellular polysaccharides	1449:1664	Grass carp intestinal microbiome thus reflects its evolutionary adaption for harvesting nutrients for an herbivore with a high-throughput nutritional strategy that is not dominated by cellulose digestion but rather the degradation of intracellular polysaccharides.
26362922	4	11	theme	different	628:636	arg1	diets					651:655	different experimental diets	628:655	different experimental diets	628:655	Comparative transcriptomes of the microbiomes of fish fed with different experimental diets indicated that the bacterial transcriptomes are influenced by host diet.
26362922	7	12	theme	carp	1263:1266	arg1	microbiome					1279:1288	the grass carp intestinal microbiome	1253:1288	the grass carp intestinal microbiome	1253:1288	These results suggest that the grass carp intestinal microbiome functions in carbohydrate turnover and fermentation, which likely provides energy for both host and microbiota.
26362922	6	13	dep	categories	1068:1077	arg1	Clusters					1037:1044	Clusters	1037:1044	Clusters of Orthologous Group	1037:1065	Predominant 'COG' (Clusters of Orthologous Group) categories in the intestinal microbiome included those for energy production and conversion, as well as carbohydrate and amino acid transport and metabolism.
26362922	8	14	theme	carp	1408:1411	arg1	microbiome					1424:1433	Grass carp intestinal microbiome	1402:1433	Grass carp intestinal microbiome	1402:1433	Grass carp intestinal microbiome thus reflects its evolutionary adaption for harvesting nutrients for an herbivore with a high-throughput nutritional strategy that is not dominated by cellulose digestion but rather the degradation of intracellular polysaccharides.
26362922	2	15	theme	grass	295:299	arg1	carp					301:304	grass carp	295:304	grass carp	295:304	Here, we sequenced the metatranscriptome of the intestinal microbiota of grass carp, Ctenopharyngodon idellus, a freshwater herbivorous fish species.
26362922	6	16	theme	amino	1189:1193	arg1	transport					1200:1208	amino acid transport	1189:1208	amino acid transport	1189:1208	Predominant 'COG' (Clusters of Orthologous Group) categories in the intestinal microbiome included those for energy production and conversion, as well as carbohydrate and amino acid transport and metabolism.
26362922	5	17	theme	hydrolase	921:929	arg1	modules					931:937	glycoside hydrolase modules	911:937	glycoside hydrolase modules targeting the side chains of noncellulosic polysaccharides	911:996	Although hydrolases and cellulosome-based systems predicted to be involved in degradation of the main chain of cellulose, xylan, mannan and pectin were identified, transcripts with glycoside hydrolase modules targeting the side chains of noncellulosic polysaccharides were more abundant.
26362922	8	18	theme	Grass	1402:1406	arg1	carp					1408:1411	Grass carp	1402:1411	Grass carp intestinal microbiome	1402:1433	Grass carp intestinal microbiome thus reflects its evolutionary adaption for harvesting nutrients for an herbivore with a high-throughput nutritional strategy that is not dominated by cellulose digestion but rather the degradation of intracellular polysaccharides.
26362922	5	19	theme	xylan	852:856	arg1	chain					832:836	the main chain	823:836	the main chain of cellulose, xylan, mannan and pectin	823:875	Although hydrolases and cellulosome-based systems predicted to be involved in degradation of the main chain of cellulose, xylan, mannan and pectin were identified, transcripts with glycoside hydrolase modules targeting the side chains of noncellulosic polysaccharides were more abundant.
26362922	8	20	theme	intracellular	1636:1648	arg1	polysaccharides					1650:1664	intracellular polysaccharides	1636:1664	intracellular polysaccharides	1636:1664	Grass carp intestinal microbiome thus reflects its evolutionary adaption for harvesting nutrients for an herbivore with a high-throughput nutritional strategy that is not dominated by cellulose digestion but rather the degradation of intracellular polysaccharides.
26362922	2	21	theme	microbiota	281:290	arg1	metatranscriptome					245:261	the metatranscriptome	241:261	the metatranscriptome of the intestinal microbiota of grass carp, Ctenopharyngodon idellus, a freshwater herbivorous fish species	241:369	Here, we sequenced the metatranscriptome of the intestinal microbiota of grass carp, Ctenopharyngodon idellus, a freshwater herbivorous fish species.
26362922	1	22	theme	fish	184:187	arg1	microbiomes					189:199	fish microbiomes	184:199	fish microbiomes	184:199	Despite the economic importance of fish, the ecology and metabolic capacity of fish microbiomes are largely unknown.
26362922	4	23	theme	microbiomes	599:609	arg1	transcriptomes					577:590	Comparative transcriptomes	565:590	Comparative transcriptomes of the microbiomes of fish fed with different experimental diets	565:655	Comparative transcriptomes of the microbiomes of fish fed with different experimental diets indicated that the bacterial transcriptomes are influenced by host diet.
26362922	1	24	theme	microbiomes	189:199	arg1	ecology					150:156	ecology	150:156	ecology	150:156	Despite the economic importance of fish, the ecology and metabolic capacity of fish microbiomes are largely unknown.
26362922	1	24	theme	microbiomes	189:199	arg1	capacity					172:179	metabolic capacity	162:179	metabolic capacity	162:179	Despite the economic importance of fish, the ecology and metabolic capacity of fish microbiomes are largely unknown.
26362922	0	25	theme	Metatranscriptomic	0:17	arg1	discovery					19:27	Metatranscriptomic discovery	0:27	Metatranscriptomic discovery of plant biomass-degrading capacity from grass carp intestinal	0:90	Metatranscriptomic discovery of plant biomass-degrading capacity from grass carp intestinal microbiomes.
26362922	3	26	theme	microbiota	452:461	arg1	composition					433:443	the bacterial composition	419:443	the bacterial composition of the microbiota	419:461	Our results confirmed previous work describing the bacterial composition of the microbiota at the phylum level as being dominated by Firmicutes, Fusobacteria, Proteobacteria and Bacteriodetes.
26362922	0	27	theme	plant	32:36	arg1	capacity					56:63	plant biomass-degrading capacity	32:63	plant biomass-degrading capacity	32:63	Metatranscriptomic discovery of plant biomass-degrading capacity from grass carp intestinal microbiomes.
26362922	5	28	theme	mannan	859:864	arg1	chain					832:836	the main chain	823:836	the main chain of cellulose, xylan, mannan and pectin	823:875	Although hydrolases and cellulosome-based systems predicted to be involved in degradation of the main chain of cellulose, xylan, mannan and pectin were identified, transcripts with glycoside hydrolase modules targeting the side chains of noncellulosic polysaccharides were more abundant.
26362922	5	29	theme	cellulosome-based	754:770	arg1	systems					772:778	cellulosome-based systems	754:778	cellulosome-based systems	754:778	Although hydrolases and cellulosome-based systems predicted to be involved in degradation of the main chain of cellulose, xylan, mannan and pectin were identified, transcripts with glycoside hydrolase modules targeting the side chains of noncellulosic polysaccharides were more abundant.
26362922	1	30	dep	ecology	150:156	arg1	the					146:148	the	146:148	the	146:148	Despite the economic importance of fish, the ecology and metabolic capacity of fish microbiomes are largely unknown.
26362922	4	31	theme	bacterial	676:684	arg1	transcriptomes					686:699	the bacterial transcriptomes	672:699	the bacterial transcriptomes	672:699	Comparative transcriptomes of the microbiomes of fish fed with different experimental diets indicated that the bacterial transcriptomes are influenced by host diet.
26362922	3	32	theme	previous	394:401	arg1	work					403:406	previous work	394:406	previous work describing the bacterial composition of the microbiota at the phylum level	394:481	Our results confirmed previous work describing the bacterial composition of the microbiota at the phylum level as being dominated by Firmicutes, Fusobacteria, Proteobacteria and Bacteriodetes.
26362922	6	33	theme	COG	1031:1033	arg1	categories					1068:1077	Predominant 'COG' (Clusters of Orthologous Group) categories	1018:1077	Predominant 'COG' (Clusters of Orthologous Group) categories in the intestinal microbiome	1018:1106	Predominant 'COG' (Clusters of Orthologous Group) categories in the intestinal microbiome included those for energy production and conversion, as well as carbohydrate and amino acid transport and metabolism.
26362922	5	34	with	transcripts	894:904	arg1	modules					931:937	glycoside hydrolase modules	911:937	glycoside hydrolase modules targeting the side chains of noncellulosic polysaccharides	911:996	Although hydrolases and cellulosome-based systems predicted to be involved in degradation of the main chain of cellulose, xylan, mannan and pectin were identified, transcripts with glycoside hydrolase modules targeting the side chains of noncellulosic polysaccharides were more abundant.
26362922	8	35	theme	nutritional	1540:1550	arg1	strategy					1552:1559	a high-throughput nutritional strategy	1522:1559	a high-throughput nutritional strategy that is not dominated by cellulose digestion but rather the degradation of intracellular polysaccharides	1522:1664	Grass carp intestinal microbiome thus reflects its evolutionary adaption for harvesting nutrients for an herbivore with a high-throughput nutritional strategy that is not dominated by cellulose digestion but rather the degradation of intracellular polysaccharides.
26362922	6	36	theme	acid	1195:1198	arg1	transport					1200:1208	amino acid transport	1189:1208	amino acid transport	1189:1208	Predominant 'COG' (Clusters of Orthologous Group) categories in the intestinal microbiome included those for energy production and conversion, as well as carbohydrate and amino acid transport and metabolism.
26362922	0	37	theme	capacity	56:63	arg1	discovery					19:27	Metatranscriptomic discovery	0:27	Metatranscriptomic discovery of plant biomass-degrading capacity from grass carp intestinal	0:90	Metatranscriptomic discovery of plant biomass-degrading capacity from grass carp intestinal microbiomes.
26362922	2	38	theme	herbivorous	346:356	arg1	microbiota					281:290	the intestinal microbiota	266:290	the intestinal microbiota of grass carp	266:304	Here, we sequenced the metatranscriptome of the intestinal microbiota of grass carp, Ctenopharyngodon idellus, a freshwater herbivorous fish species.
26362922	2	38	theme	herbivorous	346:356	arg1	species					363:369	a freshwater herbivorous fish species	333:369	a freshwater herbivorous fish species	333:369	Here, we sequenced the metatranscriptome of the intestinal microbiota of grass carp, Ctenopharyngodon idellus, a freshwater herbivorous fish species.
26362922	8	39	theme	high-throughput	1524:1538	arg1	strategy					1552:1559	a high-throughput nutritional strategy	1522:1559	a high-throughput nutritional strategy that is not dominated by cellulose digestion but rather the degradation of intracellular polysaccharides	1522:1664	Grass carp intestinal microbiome thus reflects its evolutionary adaption for harvesting nutrients for an herbivore with a high-throughput nutritional strategy that is not dominated by cellulose digestion but rather the degradation of intracellular polysaccharides.
26362922	8	40	theme	harvesting	1479:1488	arg1	nutrients					1490:1498	harvesting nutrients	1479:1498	harvesting nutrients	1479:1498	Grass carp intestinal microbiome thus reflects its evolutionary adaption for harvesting nutrients for an herbivore with a high-throughput nutritional strategy that is not dominated by cellulose digestion but rather the degradation of intracellular polysaccharides.
26362922	1	41	theme	economic	117:124	arg1	importance					126:135	the economic importance	113:135	the economic importance of fish	113:143	Despite the economic importance of fish, the ecology and metabolic capacity of fish microbiomes are largely unknown.
26362922	3	42	theme	phylum	470:475	arg1	level					477:481	the phylum level	466:481	the phylum level	466:481	Our results confirmed previous work describing the bacterial composition of the microbiota at the phylum level as being dominated by Firmicutes, Fusobacteria, Proteobacteria and Bacteriodetes.
26362922	0	43	theme	biomass-degrading	38:54	arg1	capacity					56:63	plant biomass-degrading capacity	32:63	plant biomass-degrading capacity	32:63	Metatranscriptomic discovery of plant biomass-degrading capacity from grass carp intestinal microbiomes.
26362922	6	44	theme	Predominant	1018:1028	arg1	COG					1031:1033	Predominant 'COG'	1018:1034	Predominant 'COG' (Clusters of Orthologous Group) categories in the intestinal microbiome	1018:1106	Predominant 'COG' (Clusters of Orthologous Group) categories in the intestinal microbiome included those for energy production and conversion, as well as carbohydrate and amino acid transport and metabolism.
26362922	5	45	theme	cellulose	841:849	arg1	chain					832:836	the main chain	823:836	the main chain of cellulose, xylan, mannan and pectin	823:875	Although hydrolases and cellulosome-based systems predicted to be involved in degradation of the main chain of cellulose, xylan, mannan and pectin were identified, transcripts with glycoside hydrolase modules targeting the side chains of noncellulosic polysaccharides were more abundant.
26362922	6	46	theme	Orthologous	1049:1059	arg1	Group					1061:1065	Orthologous Group	1049:1065	Orthologous Group	1049:1065	Predominant 'COG' (Clusters of Orthologous Group) categories in the intestinal microbiome included those for energy production and conversion, as well as carbohydrate and amino acid transport and metabolism.
26362922	5	47	theme	noncellulosic	968:980	arg1	polysaccharides					982:996	noncellulosic polysaccharides	968:996	noncellulosic polysaccharides	968:996	Although hydrolases and cellulosome-based systems predicted to be involved in degradation of the main chain of cellulose, xylan, mannan and pectin were identified, transcripts with glycoside hydrolase modules targeting the side chains of noncellulosic polysaccharides were more abundant.
26362922	6	48	theme	energy	1127:1132	arg1	production					1134:1143	energy production	1127:1143	energy production	1127:1143	Predominant 'COG' (Clusters of Orthologous Group) categories in the intestinal microbiome included those for energy production and conversion, as well as carbohydrate and amino acid transport and metabolism.
26362922	0	49	theme	grass	70:74	arg1	carp					76:79	grass carp intestinal	70:90	grass carp intestinal	70:90	Metatranscriptomic discovery of plant biomass-degrading capacity from grass carp intestinal microbiomes.
26362922	0	50	from	carp	76:79	arg1	discovery					19:27	Metatranscriptomic discovery	0:27	Metatranscriptomic discovery of plant biomass-degrading capacity from grass carp intestinal	0:90	Metatranscriptomic discovery of plant biomass-degrading capacity from grass carp intestinal microbiomes.
26362922	8	51	theme	cellulose	1586:1594	arg1	digestion					1596:1604	cellulose digestion	1586:1604	cellulose digestion but rather the degradation of intracellular polysaccharides	1586:1664	Grass carp intestinal microbiome thus reflects its evolutionary adaption for harvesting nutrients for an herbivore with a high-throughput nutritional strategy that is not dominated by cellulose digestion but rather the degradation of intracellular polysaccharides.
26362922	2	52	theme	fish	358:361	arg1	microbiota					281:290	the intestinal microbiota	266:290	the intestinal microbiota of grass carp	266:304	Here, we sequenced the metatranscriptome of the intestinal microbiota of grass carp, Ctenopharyngodon idellus, a freshwater herbivorous fish species.
26362922	2	52	theme	fish	358:361	arg1	species					363:369	a freshwater herbivorous fish species	333:369	a freshwater herbivorous fish species	333:369	Here, we sequenced the metatranscriptome of the intestinal microbiota of grass carp, Ctenopharyngodon idellus, a freshwater herbivorous fish species.
26362922	3	53	theme	bacterial	423:431	arg1	composition					433:443	the bacterial composition	419:443	the bacterial composition of the microbiota	419:461	Our results confirmed previous work describing the bacterial composition of the microbiota at the phylum level as being dominated by Firmicutes, Fusobacteria, Proteobacteria and Bacteriodetes.
26362922	8	54	with	herbivore	1507:1515	arg1	strategy					1552:1559	a high-throughput nutritional strategy	1522:1559	a high-throughput nutritional strategy that is not dominated by cellulose digestion but rather the degradation of intracellular polysaccharides	1522:1664	Grass carp intestinal microbiome thus reflects its evolutionary adaption for harvesting nutrients for an herbivore with a high-throughput nutritional strategy that is not dominated by cellulose digestion but rather the degradation of intracellular polysaccharides.
26362922	1	55	theme	fish	140:143	arg1	importance					126:135	the economic importance	113:135	the economic importance of fish	113:143	Despite the economic importance of fish, the ecology and metabolic capacity of fish microbiomes are largely unknown.
26362922	4	56	theme	Comparative	565:575	arg1	transcriptomes					577:590	Comparative transcriptomes	565:590	Comparative transcriptomes of the microbiomes of fish fed with different experimental diets	565:655	Comparative transcriptomes of the microbiomes of fish fed with different experimental diets indicated that the bacterial transcriptomes are influenced by host diet.
26362922	4	57	theme	host	719:722	arg1	diet					724:727	host diet	719:727	host diet	719:727	Comparative transcriptomes of the microbiomes of fish fed with different experimental diets indicated that the bacterial transcriptomes are influenced by host diet.
26362922	2	58	theme	intestinal	270:279	arg1	microbiota					281:290	the intestinal microbiota	266:290	the intestinal microbiota of grass carp	266:304	Here, we sequenced the metatranscriptome of the intestinal microbiota of grass carp, Ctenopharyngodon idellus, a freshwater herbivorous fish species.
26362922	2	58	theme	intestinal	270:279	arg1	idellus					324:330	Ctenopharyngodon idellus	307:330	Ctenopharyngodon idellus	307:330	Here, we sequenced the metatranscriptome of the intestinal microbiota of grass carp, Ctenopharyngodon idellus, a freshwater herbivorous fish species.
26362922	2	58	theme	intestinal	270:279	arg1	species					363:369	a freshwater herbivorous fish species	333:369	a freshwater herbivorous fish species	333:369	Here, we sequenced the metatranscriptome of the intestinal microbiota of grass carp, Ctenopharyngodon idellus, a freshwater herbivorous fish species.
26362922	2	59	theme	freshwater	335:344	arg1	microbiota					281:290	the intestinal microbiota	266:290	the intestinal microbiota of grass carp	266:304	Here, we sequenced the metatranscriptome of the intestinal microbiota of grass carp, Ctenopharyngodon idellus, a freshwater herbivorous fish species.
26362922	2	59	theme	freshwater	335:344	arg1	species					363:369	a freshwater herbivorous fish species	333:369	a freshwater herbivorous fish species	333:369	Here, we sequenced the metatranscriptome of the intestinal microbiota of grass carp, Ctenopharyngodon idellus, a freshwater herbivorous fish species.
26362922	2	60	theme	Ctenopharyngodon	307:322	arg1	microbiota					281:290	the intestinal microbiota	266:290	the intestinal microbiota of grass carp	266:304	Here, we sequenced the metatranscriptome of the intestinal microbiota of grass carp, Ctenopharyngodon idellus, a freshwater herbivorous fish species.
26362922	2	60	theme	Ctenopharyngodon	307:322	arg1	idellus					324:330	Ctenopharyngodon idellus	307:330	Ctenopharyngodon idellus	307:330	Here, we sequenced the metatranscriptome of the intestinal microbiota of grass carp, Ctenopharyngodon idellus, a freshwater herbivorous fish species.
26362922	5	61	theme	polysaccharides	982:996	arg1	chains					958:963	the side chains	949:963	the side chains of noncellulosic polysaccharides	949:996	Although hydrolases and cellulosome-based systems predicted to be involved in degradation of the main chain of cellulose, xylan, mannan and pectin were identified, transcripts with glycoside hydrolase modules targeting the side chains of noncellulosic polysaccharides were more abundant.
26362922	8	62	theme	intestinal	1413:1422	arg1	microbiome					1424:1433	Grass carp intestinal microbiome	1402:1433	Grass carp intestinal microbiome	1402:1433	Grass carp intestinal microbiome thus reflects its evolutionary adaption for harvesting nutrients for an herbivore with a high-throughput nutritional strategy that is not dominated by cellulose digestion but rather the degradation of intracellular polysaccharides.
26362922	4	63	theme	experimental	638:649	arg1	diets					651:655	different experimental diets	628:655	different experimental diets	628:655	Comparative transcriptomes of the microbiomes of fish fed with different experimental diets indicated that the bacterial transcriptomes are influenced by host diet.
26362922	0	64	theme	intestinal	81:90	arg1	carp					76:79	grass carp intestinal	70:90	grass carp intestinal	70:90	Metatranscriptomic discovery of plant biomass-degrading capacity from grass carp intestinal microbiomes.
26362922	6	65	theme	intestinal	1086:1095	arg1	microbiome					1097:1106	the intestinal microbiome	1082:1106	the intestinal microbiome	1082:1106	Predominant 'COG' (Clusters of Orthologous Group) categories in the intestinal microbiome included those for energy production and conversion, as well as carbohydrate and amino acid transport and metabolism.
26362922	8	66	theme	polysaccharides	1650:1664	arg1	degradation					1621:1631	the degradation	1617:1631	cellulose digestion but rather the degradation of intracellular polysaccharides	1586:1664	Grass carp intestinal microbiome thus reflects its evolutionary adaption for harvesting nutrients for an herbivore with a high-throughput nutritional strategy that is not dominated by cellulose digestion but rather the degradation of intracellular polysaccharides.
26362922	5	67	theme	glycoside	911:919	arg1	modules					931:937	glycoside hydrolase modules	911:937	glycoside hydrolase modules targeting the side chains of noncellulosic polysaccharides	911:996	Although hydrolases and cellulosome-based systems predicted to be involved in degradation of the main chain of cellulose, xylan, mannan and pectin were identified, transcripts with glycoside hydrolase modules targeting the side chains of noncellulosic polysaccharides were more abundant.
26362922	5	68	theme	pectin	870:875	arg1	chain					832:836	the main chain	823:836	the main chain of cellulose, xylan, mannan and pectin	823:875	Although hydrolases and cellulosome-based systems predicted to be involved in degradation of the main chain of cellulose, xylan, mannan and pectin were identified, transcripts with glycoside hydrolase modules targeting the side chains of noncellulosic polysaccharides were more abundant.
26362922	7	69	theme	carbohydrate	1303:1314	arg1	turnover					1316:1323	carbohydrate turnover	1303:1323	carbohydrate turnover	1303:1323	These results suggest that the grass carp intestinal microbiome functions in carbohydrate turnover and fermentation, which likely provides energy for both host and microbiota.
26483522	6	0	theme	lipid	783:787	arg1	A					789:789	lipid A	783:789	lipid A	783:789	We obtained direct evidence for LPS and lipid A using electrophoresis and differential staining.
26483522	7	1	theme	chromatography-mass	844:862	arg1	analysis					899:906	Gas chromatography-mass spectrometry (GC-MS) compositional analysis	840:906	Gas chromatography-mass spectrometry (GC-MS) compositional analysis of LPS extracts	840:922	Gas chromatography-mass spectrometry (GC-MS) compositional analysis of LPS extracts identified eight different 3-hydroxy fatty acids (3-HOFAs), 2-keto 3-deoxy-d-manno-octulosonic acid (Kdo), glucosamine, and hexose and heptose sugars, a chemical profile unique to Gram-negative LPS.
26483522	8	2	theme	molecular/structural	1137:1156	arg1	information					1158:1168	molecular/structural information	1137:1168	molecular/structural information collected from matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) MS analysis of putative intact lipid A	1137:1293	Combined with molecular/structural information collected from matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) MS analysis of putative intact lipid A, these data led us to propose a heterogeneous hexa-acylated lipid A structure (multiple-lipid A species).
26483522	11	3	theme	Gram-negative	1894:1906	arg1	plan					1913:1916	the Gram-negative cell plan	1890:1916	the Gram-negative cell plan	1890:1916	The pursuit of this question, together with studies of the structural connections between planctomycete LPS and peptidoglycans, will shed more light on what appears to be a planctomycete variation on the Gram-negative cell plan.
26483522	11	4	theme	planctomycete	1863:1875	arg1	variation					1877:1885	a planctomycete variation	1861:1885	a planctomycete variation on the Gram-negative cell plan	1861:1916	The pursuit of this question, together with studies of the structural connections between planctomycete LPS and peptidoglycans, will shed more light on what appears to be a planctomycete variation on the Gram-negative cell plan.
26483522	11	4	theme	planctomycete	1863:1875	arg1	what					1842:1845	what	1842:1845	what	1842:1845	The pursuit of this question, together with studies of the structural connections between planctomycete LPS and peptidoglycans, will shed more light on what appears to be a planctomycete variation on the Gram-negative cell plan.
26483522	9	5	theme	previous	1419:1426	arg1	reports					1428:1434	previous reports	1419:1434	previous reports of G. obscuriglobus whole-cell fatty acid (FA)	1419:1481	We also confirmed previous reports of G. obscuriglobus whole-cell fatty acid (FA) and sterol compositions and detected a novel polyunsaturated FA (PUFA).
26483522	15	6	theme	OM	2499:2500	arg1	presence					2484:2491	the presence	2480:2491	the presence of an OM	2480:2500	Here, we report direct evidence of lipopolysaccharide in the planctomycete G. obscuriglobus, suggesting the presence of an OM and supporting the proposal that the planctomycete cell envelope is an extension of the canonical Gram-negative plan.
26483522	16	7	theme	future	2771:2776	arg1	investigation					2778:2790	future investigation	2771:2790	future investigation	2771:2790	This interpretation features a convoluted cytoplasmic membrane and expanded periplasmic space, the functions of which provide an intriguing avenue for future investigation.
26483522	4	8	theme	biochemical	611:621	arg1	verification					623:634	no biochemical verification	608:634	no biochemical verification	608:634	Despite genomic evidence for an OM in planctomycetes, no biochemical verification has been reported.
26483522	10	9	dep	confirmation	1559:1570	arg1	OM					1602:1603	an OM	1599:1603	an OM	1599:1603	Our confirmation of LPS, and by implication an OM, in G. obscuriglobus raises the possibility that other planctomycetes possess an OM.
26483522	14	10	theme	cell	2302:2305	arg1	wall					2307:2310	a more conventional cell wall	2282:2310	a more conventional cell wall	2282:2310	However, the very recent detection of peptidoglycan in planctomycete species has provided evidence for a more conventional cell wall and raised questions about other elements of the cell envelope.
26483522	8	11	theme	matrix-assisted	1185:1199	arg1	ionization-time					1218:1232	matrix-assisted laser desorption ionization-time	1185:1232	matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) MS analysis of putative intact lipid A	1185:1293	Combined with molecular/structural information collected from matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) MS analysis of putative intact lipid A, these data led us to propose a heterogeneous hexa-acylated lipid A structure (multiple-lipid A species).
26483522	8	12	theme	analysis	1259:1266	arg1	ionization-time					1218:1232	matrix-assisted laser desorption ionization-time	1185:1232	matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) MS analysis of putative intact lipid A	1185:1293	Combined with molecular/structural information collected from matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) MS analysis of putative intact lipid A, these data led us to propose a heterogeneous hexa-acylated lipid A structure (multiple-lipid A species).
26483522	10	13	theme	LPS	1575:1577	arg1	confirmation					1559:1570	Our confirmation	1555:1570	Our confirmation of LPS	1555:1577	Our confirmation of LPS, and by implication an OM, in G. obscuriglobus raises the possibility that other planctomycetes possess an OM.
26483522	10	13	theme	LPS	1575:1577	arg1	implication					1587:1597	implication	1587:1597	implication	1587:1597	Our confirmation of LPS, and by implication an OM, in G. obscuriglobus raises the possibility that other planctomycetes possess an OM.
26483522	11	14	theme	structural	1749:1758	arg1	connections					1760:1770	the structural connections	1745:1770	the structural connections between planctomycete LPS and peptidoglycans	1745:1815	The pursuit of this question, together with studies of the structural connections between planctomycete LPS and peptidoglycans, will shed more light on what appears to be a planctomycete variation on the Gram-negative cell plan.
26483522	15	15	theme	planctomycete	2539:2551	arg1	envelope					2558:2565	the planctomycete cell envelope	2535:2565	the planctomycete cell envelope	2535:2565	Here, we report direct evidence of lipopolysaccharide in the planctomycete G. obscuriglobus, suggesting the presence of an OM and supporting the proposal that the planctomycete cell envelope is an extension of the canonical Gram-negative plan.
26483522	15	15	theme	planctomycete	2539:2551	arg1	extension					2573:2581	an extension	2570:2581	an extension of the canonical Gram-negative plan	2570:2617	Here, we report direct evidence of lipopolysaccharide in the planctomycete G. obscuriglobus, suggesting the presence of an OM and supporting the proposal that the planctomycete cell envelope is an extension of the canonical Gram-negative plan.
26483522	1	16	theme	many	205:208	arg1	properties					227:236	many unusual cellular properties	205:236	many unusual cellular properties	205:236	UNLABELLED Planctomycete bacteria possess many unusual cellular properties, contributing to a cell plan long considered to be unique among the bacteria.
26483522	8	17	theme	desorption	1207:1216	arg1	ionization-time					1218:1232	matrix-assisted laser desorption ionization-time	1185:1232	matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) MS analysis of putative intact lipid A	1185:1293	Combined with molecular/structural information collected from matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) MS analysis of putative intact lipid A, these data led us to propose a heterogeneous hexa-acylated lipid A structure (multiple-lipid A species).
26483522	8	18	theme	putative	1271:1278	arg1	A					1293:1293	putative intact lipid A	1271:1293	putative intact lipid A	1271:1293	Combined with molecular/structural information collected from matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) MS analysis of putative intact lipid A, these data led us to propose a heterogeneous hexa-acylated lipid A structure (multiple-lipid A species).
26483522	15	19	theme	direct	2392:2397	arg1	evidence					2399:2406	direct evidence	2392:2406	direct evidence of lipopolysaccharide in the planctomycete G. obscuriglobus	2392:2466	Here, we report direct evidence of lipopolysaccharide in the planctomycete G. obscuriglobus, suggesting the presence of an OM and supporting the proposal that the planctomycete cell envelope is an extension of the canonical Gram-negative plan.
26483522	13	20	theme	planctomycete	2062:2074	arg1	bacteria					2076:2083	planctomycete bacteria	2062:2083	planctomycete bacteria	2062:2083	For 25 years, the envelope of planctomycete bacteria has been considered a unique exception, as it lacks peptidoglycan and an outer membrane (OM).
26483522	9	21	theme	fatty	1467:1471	arg1	FA					1479:1480	FA	1479:1480	FA	1479:1480	We also confirmed previous reports of G. obscuriglobus whole-cell fatty acid (FA) and sterol compositions and detected a novel polyunsaturated FA (PUFA).
26483522	9	21	theme	fatty	1467:1471	arg1	acid					1473:1476	whole-cell fatty acid	1456:1476	G. obscuriglobus whole-cell fatty acid (FA)	1439:1481	We also confirmed previous reports of G. obscuriglobus whole-cell fatty acid (FA) and sterol compositions and detected a novel polyunsaturated FA (PUFA).
26483522	16	22	theme	convoluted	2651:2660	arg1	membrane					2674:2681	a convoluted cytoplasmic membrane	2649:2681	a convoluted cytoplasmic membrane	2649:2681	This interpretation features a convoluted cytoplasmic membrane and expanded periplasmic space, the functions of which provide an intriguing avenue for future investigation.
26483522	8	23	theme	lipid	1287:1291	arg1	A					1293:1293	putative intact lipid A	1271:1293	putative intact lipid A	1271:1293	Combined with molecular/structural information collected from matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) MS analysis of putative intact lipid A, these data led us to propose a heterogeneous hexa-acylated lipid A structure (multiple-lipid A species).
26483522	0	24	theme	Classical	123:131	arg1	Membrane					153:160	the Classical Gram-Negative Outer Membrane	119:160	the Classical Gram-Negative Outer Membrane	119:160	Global and Targeted Lipid Analysis of Gemmata obscuriglobus Reveals the Presence of Lipopolysaccharide, a Signature of the Classical Gram-Negative Outer Membrane.
26483522	11	25	from	variation	1877:1885	arg1	plan					1913:1916	the Gram-negative cell plan	1890:1916	the Gram-negative cell plan	1890:1916	The pursuit of this question, together with studies of the structural connections between planctomycete LPS and peptidoglycans, will shed more light on what appears to be a planctomycete variation on the Gram-negative cell plan.
26483522	0	26	theme	Outer	147:151	arg1	Membrane					153:160	the Classical Gram-Negative Outer Membrane	119:160	the Classical Gram-Negative Outer Membrane	119:160	Global and Targeted Lipid Analysis of Gemmata obscuriglobus Reveals the Presence of Lipopolysaccharide, a Signature of the Classical Gram-Negative Outer Membrane.
26483522	11	27	theme	cell	1908:1911	arg1	plan					1913:1916	the Gram-negative cell plan	1890:1916	the Gram-negative cell plan	1890:1916	The pursuit of this question, together with studies of the structural connections between planctomycete LPS and peptidoglycans, will shed more light on what appears to be a planctomycete variation on the Gram-negative cell plan.
26483522	7	28	theme	2-keto	984:989	arg1	Kdo					1025:1027	Kdo	1025:1027	Kdo	1025:1027	Gas chromatography-mass spectrometry (GC-MS) compositional analysis of LPS extracts identified eight different 3-hydroxy fatty acids (3-HOFAs), 2-keto 3-deoxy-d-manno-octulosonic acid (Kdo), glucosamine, and hexose and heptose sugars, a chemical profile unique to Gram-negative LPS.
26483522	7	28	theme	2-keto	984:989	arg1	acid					1019:1022	2-keto 3-deoxy-d-manno-octulosonic acid	984:1022	2-keto 3-deoxy-d-manno-octulosonic acid (Kdo)	984:1028	Gas chromatography-mass spectrometry (GC-MS) compositional analysis of LPS extracts identified eight different 3-hydroxy fatty acids (3-HOFAs), 2-keto 3-deoxy-d-manno-octulosonic acid (Kdo), glucosamine, and hexose and heptose sugars, a chemical profile unique to Gram-negative LPS.
26483522	7	28	theme	2-keto	984:989	arg1	acids					967:971	3-hydroxy fatty acids	951:971	eight different 3-hydroxy fatty acids (3-HOFAs)	935:981	Gas chromatography-mass spectrometry (GC-MS) compositional analysis of LPS extracts identified eight different 3-hydroxy fatty acids (3-HOFAs), 2-keto 3-deoxy-d-manno-octulosonic acid (Kdo), glucosamine, and hexose and heptose sugars, a chemical profile unique to Gram-negative LPS.
26483522	13	29	dep	exception	2114:2122	arg1	lacks					2131:2135	lacks	2131:2135	lacks peptidoglycan and an outer membrane (OM)	2131:2176	For 25 years, the envelope of planctomycete bacteria has been considered a unique exception, as it lacks peptidoglycan and an outer membrane (OM).
26483522	9	30	theme	sterol	1487:1492	arg1	compositions					1494:1505	sterol compositions	1487:1505	sterol compositions	1487:1505	We also confirmed previous reports of G. obscuriglobus whole-cell fatty acid (FA) and sterol compositions and detected a novel polyunsaturated FA (PUFA).
26483522	12	31	theme	cell	2007:2010	arg1	structure					2021:2029	their cell envelope structure	2001:2029	their cell envelope structure	2001:2029	IMPORTANCE Bacterial species are classified as Gram positive or negative based on their cell envelope structure.
26483522	5	32	theme	Gemmata	720:726	arg1	obscuriglobus					728:740	the planctomycete Gemmata obscuriglobus	702:740	the planctomycete Gemmata obscuriglobus	702:740	We attempted to detect and characterize LPS in the planctomycete Gemmata obscuriglobus.
26483522	14	33	theme	planctomycete	2234:2246	arg1	species					2248:2254	planctomycete species	2234:2254	planctomycete species	2234:2254	However, the very recent detection of peptidoglycan in planctomycete species has provided evidence for a more conventional cell wall and raised questions about other elements of the cell envelope.
26483522	7	34	theme	fatty	961:965	arg1	acid					1019:1022	2-keto 3-deoxy-d-manno-octulosonic acid	984:1022	2-keto 3-deoxy-d-manno-octulosonic acid (Kdo)	984:1028	Gas chromatography-mass spectrometry (GC-MS) compositional analysis of LPS extracts identified eight different 3-hydroxy fatty acids (3-HOFAs), 2-keto 3-deoxy-d-manno-octulosonic acid (Kdo), glucosamine, and hexose and heptose sugars, a chemical profile unique to Gram-negative LPS.
26483522	7	34	theme	fatty	961:965	arg1	glucosamine					1031:1041	glucosamine	1031:1041	glucosamine	1031:1041	Gas chromatography-mass spectrometry (GC-MS) compositional analysis of LPS extracts identified eight different 3-hydroxy fatty acids (3-HOFAs), 2-keto 3-deoxy-d-manno-octulosonic acid (Kdo), glucosamine, and hexose and heptose sugars, a chemical profile unique to Gram-negative LPS.
26483522	7	34	theme	fatty	961:965	arg1	hexose					1048:1053	hexose	1048:1053	hexose	1048:1053	Gas chromatography-mass spectrometry (GC-MS) compositional analysis of LPS extracts identified eight different 3-hydroxy fatty acids (3-HOFAs), 2-keto 3-deoxy-d-manno-octulosonic acid (Kdo), glucosamine, and hexose and heptose sugars, a chemical profile unique to Gram-negative LPS.
26483522	7	34	theme	fatty	961:965	arg1	3-HOFAs					974:980	3-HOFAs	974:980	3-HOFAs	974:980	Gas chromatography-mass spectrometry (GC-MS) compositional analysis of LPS extracts identified eight different 3-hydroxy fatty acids (3-HOFAs), 2-keto 3-deoxy-d-manno-octulosonic acid (Kdo), glucosamine, and hexose and heptose sugars, a chemical profile unique to Gram-negative LPS.
26483522	7	34	theme	fatty	961:965	arg1	acids					967:971	3-hydroxy fatty acids	951:971	eight different 3-hydroxy fatty acids (3-HOFAs)	935:981	Gas chromatography-mass spectrometry (GC-MS) compositional analysis of LPS extracts identified eight different 3-hydroxy fatty acids (3-HOFAs), 2-keto 3-deoxy-d-manno-octulosonic acid (Kdo), glucosamine, and hexose and heptose sugars, a chemical profile unique to Gram-negative LPS.
26483522	1	35	theme	UNLABELLED	163:172	arg1	bacteria					188:195	UNLABELLED Planctomycete bacteria	163:195	UNLABELLED Planctomycete bacteria	163:195	UNLABELLED Planctomycete bacteria possess many unusual cellular properties, contributing to a cell plan long considered to be unique among the bacteria.
26483522	8	36	theme	heterogeneous	1327:1339	arg1	structure					1363:1371	a heterogeneous hexa-acylated lipid A structure	1325:1371	a heterogeneous hexa-acylated lipid A structure (multiple-lipid A species)	1325:1398	Combined with molecular/structural information collected from matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) MS analysis of putative intact lipid A, these data led us to propose a heterogeneous hexa-acylated lipid A structure (multiple-lipid A species).
26483522	8	36	theme	heterogeneous	1327:1339	arg1	species					1391:1397	multiple-lipid A species	1374:1397	multiple-lipid A species	1374:1397	Combined with molecular/structural information collected from matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) MS analysis of putative intact lipid A, these data led us to propose a heterogeneous hexa-acylated lipid A structure (multiple-lipid A species).
26483522	14	37	theme	peptidoglycan	2217:2229	arg1	detection					2204:2212	the very recent detection	2188:2212	the very recent detection of peptidoglycan in planctomycete species	2188:2254	However, the very recent detection of peptidoglycan in planctomycete species has provided evidence for a more conventional cell wall and raised questions about other elements of the cell envelope.
26483522	0	38	theme	Lipopolysaccharide	84:101	arg1	Presence					72:79	the Presence	68:79	the Presence of Lipopolysaccharide, a Signature of the Classical Gram-Negative Outer Membrane	68:160	Global and Targeted Lipid Analysis of Gemmata obscuriglobus Reveals the Presence of Lipopolysaccharide, a Signature of the Classical Gram-Negative Outer Membrane.
26483522	7	39	theme	different	941:949	arg1	acid					1019:1022	2-keto 3-deoxy-d-manno-octulosonic acid	984:1022	2-keto 3-deoxy-d-manno-octulosonic acid (Kdo)	984:1028	Gas chromatography-mass spectrometry (GC-MS) compositional analysis of LPS extracts identified eight different 3-hydroxy fatty acids (3-HOFAs), 2-keto 3-deoxy-d-manno-octulosonic acid (Kdo), glucosamine, and hexose and heptose sugars, a chemical profile unique to Gram-negative LPS.
26483522	7	39	theme	different	941:949	arg1	glucosamine					1031:1041	glucosamine	1031:1041	glucosamine	1031:1041	Gas chromatography-mass spectrometry (GC-MS) compositional analysis of LPS extracts identified eight different 3-hydroxy fatty acids (3-HOFAs), 2-keto 3-deoxy-d-manno-octulosonic acid (Kdo), glucosamine, and hexose and heptose sugars, a chemical profile unique to Gram-negative LPS.
26483522	7	39	theme	different	941:949	arg1	hexose					1048:1053	hexose	1048:1053	hexose	1048:1053	Gas chromatography-mass spectrometry (GC-MS) compositional analysis of LPS extracts identified eight different 3-hydroxy fatty acids (3-HOFAs), 2-keto 3-deoxy-d-manno-octulosonic acid (Kdo), glucosamine, and hexose and heptose sugars, a chemical profile unique to Gram-negative LPS.
26483522	7	39	theme	different	941:949	arg1	3-HOFAs					974:980	3-HOFAs	974:980	3-HOFAs	974:980	Gas chromatography-mass spectrometry (GC-MS) compositional analysis of LPS extracts identified eight different 3-hydroxy fatty acids (3-HOFAs), 2-keto 3-deoxy-d-manno-octulosonic acid (Kdo), glucosamine, and hexose and heptose sugars, a chemical profile unique to Gram-negative LPS.
26483522	7	39	theme	different	941:949	arg1	acids					967:971	3-hydroxy fatty acids	951:971	eight different 3-hydroxy fatty acids (3-HOFAs)	935:981	Gas chromatography-mass spectrometry (GC-MS) compositional analysis of LPS extracts identified eight different 3-hydroxy fatty acids (3-HOFAs), 2-keto 3-deoxy-d-manno-octulosonic acid (Kdo), glucosamine, and hexose and heptose sugars, a chemical profile unique to Gram-negative LPS.
26483522	9	40	theme	polyunsaturated	1528:1542	arg1	FA					1544:1545	a novel polyunsaturated FA	1520:1545	a novel polyunsaturated FA (PUFA)	1520:1552	We also confirmed previous reports of G. obscuriglobus whole-cell fatty acid (FA) and sterol compositions and detected a novel polyunsaturated FA (PUFA).
26483522	9	40	theme	polyunsaturated	1528:1542	arg1	PUFA					1548:1551	PUFA	1548:1551	PUFA	1548:1551	We also confirmed previous reports of G. obscuriglobus whole-cell fatty acid (FA) and sterol compositions and detected a novel polyunsaturated FA (PUFA).
26483522	8	41	theme	lipid	1355:1359	arg1	structure					1363:1371	a heterogeneous hexa-acylated lipid A structure	1325:1371	a heterogeneous hexa-acylated lipid A structure (multiple-lipid A species)	1325:1398	Combined with molecular/structural information collected from matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) MS analysis of putative intact lipid A, these data led us to propose a heterogeneous hexa-acylated lipid A structure (multiple-lipid A species).
26483522	8	41	theme	lipid	1355:1359	arg1	species					1391:1397	multiple-lipid A species	1374:1397	multiple-lipid A species	1374:1397	Combined with molecular/structural information collected from matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) MS analysis of putative intact lipid A, these data led us to propose a heterogeneous hexa-acylated lipid A structure (multiple-lipid A species).
26483522	2	42	theme	cell	400:403	arg1	plan					405:408	a modified Gram-negative cell plan	375:408	a modified Gram-negative cell plan	375:408	However, data from recent studies are more consistent with a modified Gram-negative cell plan.
26483522	15	43	theme	planctomycete	2437:2449	arg1	obscuriglobus					2454:2466	the planctomycete G. obscuriglobus	2433:2466	the planctomycete G. obscuriglobus	2433:2466	Here, we report direct evidence of lipopolysaccharide in the planctomycete G. obscuriglobus, suggesting the presence of an OM and supporting the proposal that the planctomycete cell envelope is an extension of the canonical Gram-negative plan.
26483522	2	44	theme	modified	377:384	arg1	plan					405:408	a modified Gram-negative cell plan	375:408	a modified Gram-negative cell plan	375:408	However, data from recent studies are more consistent with a modified Gram-negative cell plan.
26483522	0	45	theme	Global	0:5	arg1	Analysis					26:33	Global and Targeted Lipid Analysis	0:33	Global and Targeted Lipid Analysis of Gemmata obscuriglobus	0:58	Global and Targeted Lipid Analysis of Gemmata obscuriglobus Reveals the Presence of Lipopolysaccharide, a Signature of the Classical Gram-Negative Outer Membrane.
26483522	7	46	theme	LPS	911:913	arg1	extracts					915:922	LPS extracts	911:922	LPS extracts	911:922	Gas chromatography-mass spectrometry (GC-MS) compositional analysis of LPS extracts identified eight different 3-hydroxy fatty acids (3-HOFAs), 2-keto 3-deoxy-d-manno-octulosonic acid (Kdo), glucosamine, and hexose and heptose sugars, a chemical profile unique to Gram-negative LPS.
26483522	14	47	from	detection	2204:2212	arg1	species					2248:2254	planctomycete species	2234:2254	planctomycete species	2234:2254	However, the very recent detection of peptidoglycan in planctomycete species has provided evidence for a more conventional cell wall and raised questions about other elements of the cell envelope.
26483522	8	48	theme	multiple-lipid	1374:1387	arg1	structure					1363:1371	a heterogeneous hexa-acylated lipid A structure	1325:1371	a heterogeneous hexa-acylated lipid A structure (multiple-lipid A species)	1325:1398	Combined with molecular/structural information collected from matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) MS analysis of putative intact lipid A, these data led us to propose a heterogeneous hexa-acylated lipid A structure (multiple-lipid A species).
26483522	8	48	theme	multiple-lipid	1374:1387	arg1	species					1391:1397	multiple-lipid A species	1374:1397	multiple-lipid A species	1374:1397	Combined with molecular/structural information collected from matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) MS analysis of putative intact lipid A, these data led us to propose a heterogeneous hexa-acylated lipid A structure (multiple-lipid A species).
26483522	0	49	theme	Targeted	11:18	arg1	Analysis					26:33	Global and Targeted Lipid Analysis	0:33	Global and Targeted Lipid Analysis of Gemmata obscuriglobus	0:58	Global and Targeted Lipid Analysis of Gemmata obscuriglobus Reveals the Presence of Lipopolysaccharide, a Signature of the Classical Gram-Negative Outer Membrane.
26483522	15	50	from	obscuriglobus	2454:2466	arg1	evidence					2399:2406	direct evidence	2392:2406	direct evidence of lipopolysaccharide in the planctomycete G. obscuriglobus	2392:2466	Here, we report direct evidence of lipopolysaccharide in the planctomycete G. obscuriglobus, suggesting the presence of an OM and supporting the proposal that the planctomycete cell envelope is an extension of the canonical Gram-negative plan.
26483522	1	51	contain	possess	197:203	arg2	properties					227:236	many unusual cellular properties	205:236	many unusual cellular properties	205:236	UNLABELLED Planctomycete bacteria possess many unusual cellular properties, contributing to a cell plan long considered to be unique among the bacteria.
26483522	1	51	contain	possess	197:203	arg1	bacteria					188:195	UNLABELLED Planctomycete bacteria	163:195	UNLABELLED Planctomycete bacteria	163:195	UNLABELLED Planctomycete bacteria possess many unusual cellular properties, contributing to a cell plan long considered to be unique among the bacteria.
26483522	9	52	dep	G.	1439:1440	arg1	FA					1479:1480	FA	1479:1480	FA	1479:1480	We also confirmed previous reports of G. obscuriglobus whole-cell fatty acid (FA) and sterol compositions and detected a novel polyunsaturated FA (PUFA).
26483522	9	52	dep	G.	1439:1440	arg1	obscuriglobus					1442:1454	G. obscuriglobus whole-cell fatty acid (FA)	1439:1481	G. obscuriglobus whole-cell fatty acid (FA)	1439:1481	We also confirmed previous reports of G. obscuriglobus whole-cell fatty acid (FA) and sterol compositions and detected a novel polyunsaturated FA (PUFA).
26483522	9	52	dep	G.	1439:1440	arg1	acid					1473:1476	whole-cell fatty acid	1456:1476	G. obscuriglobus whole-cell fatty acid (FA)	1439:1481	We also confirmed previous reports of G. obscuriglobus whole-cell fatty acid (FA) and sterol compositions and detected a novel polyunsaturated FA (PUFA).
26483522	15	53	dep	report	2385:2390	arg1	supporting					2506:2515	supporting	2506:2515	supporting the proposal that the planctomycete cell envelope is an extension of the canonical Gram-negative plan	2506:2617	Here, we report direct evidence of lipopolysaccharide in the planctomycete G. obscuriglobus, suggesting the presence of an OM and supporting the proposal that the planctomycete cell envelope is an extension of the canonical Gram-negative plan.
26483522	15	53	dep	report	2385:2390	arg1	suggesting					2469:2478	suggesting	2469:2478	suggesting the presence of an OM	2469:2500	Here, we report direct evidence of lipopolysaccharide in the planctomycete G. obscuriglobus, suggesting the presence of an OM and supporting the proposal that the planctomycete cell envelope is an extension of the canonical Gram-negative plan.
26483522	3	54	theme	plan	446:449	arg1	feature					417:423	A key feature	411:423	A key feature of the Gram-negative plan	411:449	A key feature of the Gram-negative plan is the presence of an outer membrane (OM), for which lipopolysaccharide (LPS) is a signature molecule.
26483522	3	54	theme	plan	446:449	arg1	presence					458:465	the presence	454:465	the presence of an outer membrane (OM), for which lipopolysaccharide (LPS) is a signature molecule	454:551	A key feature of the Gram-negative plan is the presence of an outer membrane (OM), for which lipopolysaccharide (LPS) is a signature molecule.
26483522	14	55	theme	envelope	2366:2373	arg1	elements					2345:2352	other elements	2339:2352	other elements of the cell envelope	2339:2373	However, the very recent detection of peptidoglycan in planctomycete species has provided evidence for a more conventional cell wall and raised questions about other elements of the cell envelope.
26483522	3	56	theme	outer	473:477	arg1	OM					489:490	OM	489:490	OM	489:490	A key feature of the Gram-negative plan is the presence of an outer membrane (OM), for which lipopolysaccharide (LPS) is a signature molecule.
26483522	3	56	theme	outer	473:477	arg1	membrane					479:486	an outer membrane	470:486	an outer membrane (OM)	470:491	A key feature of the Gram-negative plan is the presence of an outer membrane (OM), for which lipopolysaccharide (LPS) is a signature molecule.
26483522	0	57	theme	Gemmata	38:44	arg1	obscuriglobus					46:58	Gemmata obscuriglobus	38:58	Gemmata obscuriglobus	38:58	Global and Targeted Lipid Analysis of Gemmata obscuriglobus Reveals the Presence of Lipopolysaccharide, a Signature of the Classical Gram-Negative Outer Membrane.
26483522	10	58	theme	other	1654:1658	arg1	planctomycetes					1660:1673	other planctomycetes	1654:1673	other planctomycetes	1654:1673	Our confirmation of LPS, and by implication an OM, in G. obscuriglobus raises the possibility that other planctomycetes possess an OM.
26483522	4	59	theme	genomic	562:568	arg1	evidence					570:577	genomic evidence	562:577	genomic evidence for an OM in planctomycetes	562:605	Despite genomic evidence for an OM in planctomycetes, no biochemical verification has been reported.
26483522	7	60	theme	compositional	885:897	arg1	analysis					899:906	Gas chromatography-mass spectrometry (GC-MS) compositional analysis	840:906	Gas chromatography-mass spectrometry (GC-MS) compositional analysis of LPS extracts	840:922	Gas chromatography-mass spectrometry (GC-MS) compositional analysis of LPS extracts identified eight different 3-hydroxy fatty acids (3-HOFAs), 2-keto 3-deoxy-d-manno-octulosonic acid (Kdo), glucosamine, and hexose and heptose sugars, a chemical profile unique to Gram-negative LPS.
26483522	0	61	theme	obscuriglobus	46:58	arg1	Analysis					26:33	Global and Targeted Lipid Analysis	0:33	Global and Targeted Lipid Analysis of Gemmata obscuriglobus	0:58	Global and Targeted Lipid Analysis of Gemmata obscuriglobus Reveals the Presence of Lipopolysaccharide, a Signature of the Classical Gram-Negative Outer Membrane.
26483522	12	62	theme	Bacterial	1930:1938	arg1	negative					1983:1990	negative	1983:1990	negative	1983:1990	IMPORTANCE Bacterial species are classified as Gram positive or negative based on their cell envelope structure.
26483522	12	62	theme	Bacterial	1930:1938	arg1	positive					1971:1978	positive	1971:1978	positive	1971:1978	IMPORTANCE Bacterial species are classified as Gram positive or negative based on their cell envelope structure.
26483522	12	62	theme	Bacterial	1930:1938	arg1	species					1940:1946	IMPORTANCE Bacterial species	1919:1946	IMPORTANCE Bacterial species	1919:1946	IMPORTANCE Bacterial species are classified as Gram positive or negative based on their cell envelope structure.
26483522	7	63	theme	unique	1094:1099	arg1	profile					1086:1092	a chemical profile	1075:1092	a chemical profile unique to Gram-negative LPS	1075:1120	Gas chromatography-mass spectrometry (GC-MS) compositional analysis of LPS extracts identified eight different 3-hydroxy fatty acids (3-HOFAs), 2-keto 3-deoxy-d-manno-octulosonic acid (Kdo), glucosamine, and hexose and heptose sugars, a chemical profile unique to Gram-negative LPS.
26483522	7	63	theme	unique	1094:1099	arg1	sugars					1067:1072	heptose sugars	1059:1072	heptose sugars	1059:1072	Gas chromatography-mass spectrometry (GC-MS) compositional analysis of LPS extracts identified eight different 3-hydroxy fatty acids (3-HOFAs), 2-keto 3-deoxy-d-manno-octulosonic acid (Kdo), glucosamine, and hexose and heptose sugars, a chemical profile unique to Gram-negative LPS.
26483522	7	64	theme	GC-MS	878:882	arg1	analysis					899:906	Gas chromatography-mass spectrometry (GC-MS) compositional analysis	840:906	Gas chromatography-mass spectrometry (GC-MS) compositional analysis of LPS extracts	840:922	Gas chromatography-mass spectrometry (GC-MS) compositional analysis of LPS extracts identified eight different 3-hydroxy fatty acids (3-HOFAs), 2-keto 3-deoxy-d-manno-octulosonic acid (Kdo), glucosamine, and hexose and heptose sugars, a chemical profile unique to Gram-negative LPS.
26483522	1	65	theme	cellular	218:225	arg1	properties					227:236	many unusual cellular properties	205:236	many unusual cellular properties	205:236	UNLABELLED Planctomycete bacteria possess many unusual cellular properties, contributing to a cell plan long considered to be unique among the bacteria.
26483522	14	66	theme	conventional	2289:2300	arg1	wall					2307:2310	a more conventional cell wall	2282:2310	a more conventional cell wall	2282:2310	However, the very recent detection of peptidoglycan in planctomycete species has provided evidence for a more conventional cell wall and raised questions about other elements of the cell envelope.
26483522	7	67	theme	chemical	1077:1084	arg1	profile					1086:1092	a chemical profile	1075:1092	a chemical profile unique to Gram-negative LPS	1075:1120	Gas chromatography-mass spectrometry (GC-MS) compositional analysis of LPS extracts identified eight different 3-hydroxy fatty acids (3-HOFAs), 2-keto 3-deoxy-d-manno-octulosonic acid (Kdo), glucosamine, and hexose and heptose sugars, a chemical profile unique to Gram-negative LPS.
26483522	7	67	theme	chemical	1077:1084	arg1	sugars					1067:1072	heptose sugars	1059:1072	heptose sugars	1059:1072	Gas chromatography-mass spectrometry (GC-MS) compositional analysis of LPS extracts identified eight different 3-hydroxy fatty acids (3-HOFAs), 2-keto 3-deoxy-d-manno-octulosonic acid (Kdo), glucosamine, and hexose and heptose sugars, a chemical profile unique to Gram-negative LPS.
26483522	7	68	theme	spectrometry	864:875	arg1	analysis					899:906	Gas chromatography-mass spectrometry (GC-MS) compositional analysis	840:906	Gas chromatography-mass spectrometry (GC-MS) compositional analysis of LPS extracts	840:922	Gas chromatography-mass spectrometry (GC-MS) compositional analysis of LPS extracts identified eight different 3-hydroxy fatty acids (3-HOFAs), 2-keto 3-deoxy-d-manno-octulosonic acid (Kdo), glucosamine, and hexose and heptose sugars, a chemical profile unique to Gram-negative LPS.
26483522	13	69	theme	unique	2107:2112	arg1	exception					2114:2122	a unique exception	2105:2122	a unique exception	2105:2122	For 25 years, the envelope of planctomycete bacteria has been considered a unique exception, as it lacks peptidoglycan and an outer membrane (OM).
26483522	2	70	from	studies	342:348	arg1	data					325:328	data	325:328	data from recent studies	325:348	However, data from recent studies are more consistent with a modified Gram-negative cell plan.
26483522	16	71	theme	intriguing	2749:2758	arg1	avenue					2760:2765	an intriguing avenue	2746:2765	an intriguing avenue for future investigation	2746:2790	This interpretation features a convoluted cytoplasmic membrane and expanded periplasmic space, the functions of which provide an intriguing avenue for future investigation.
26483522	7	72	theme	Gas	840:842	arg1	analysis					899:906	Gas chromatography-mass spectrometry (GC-MS) compositional analysis	840:906	Gas chromatography-mass spectrometry (GC-MS) compositional analysis of LPS extracts	840:922	Gas chromatography-mass spectrometry (GC-MS) compositional analysis of LPS extracts identified eight different 3-hydroxy fatty acids (3-HOFAs), 2-keto 3-deoxy-d-manno-octulosonic acid (Kdo), glucosamine, and hexose and heptose sugars, a chemical profile unique to Gram-negative LPS.
26483522	1	73	theme	Planctomycete	174:186	arg1	bacteria					188:195	UNLABELLED Planctomycete bacteria	163:195	UNLABELLED Planctomycete bacteria	163:195	UNLABELLED Planctomycete bacteria possess many unusual cellular properties, contributing to a cell plan long considered to be unique among the bacteria.
26483522	8	74	theme	MALDI-TOF	1245:1253	arg1	analysis					1259:1266	flight (MALDI-TOF) MS analysis	1237:1266	flight (MALDI-TOF) MS analysis of putative intact lipid A	1237:1293	Combined with molecular/structural information collected from matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) MS analysis of putative intact lipid A, these data led us to propose a heterogeneous hexa-acylated lipid A structure (multiple-lipid A species).
26483522	7	75	theme	heptose	1059:1065	arg1	profile					1086:1092	a chemical profile	1075:1092	a chemical profile unique to Gram-negative LPS	1075:1120	Gas chromatography-mass spectrometry (GC-MS) compositional analysis of LPS extracts identified eight different 3-hydroxy fatty acids (3-HOFAs), 2-keto 3-deoxy-d-manno-octulosonic acid (Kdo), glucosamine, and hexose and heptose sugars, a chemical profile unique to Gram-negative LPS.
26483522	7	75	theme	heptose	1059:1065	arg1	sugars					1067:1072	heptose sugars	1059:1072	heptose sugars	1059:1072	Gas chromatography-mass spectrometry (GC-MS) compositional analysis of LPS extracts identified eight different 3-hydroxy fatty acids (3-HOFAs), 2-keto 3-deoxy-d-manno-octulosonic acid (Kdo), glucosamine, and hexose and heptose sugars, a chemical profile unique to Gram-negative LPS.
26483522	6	76	theme	direct	755:760	arg1	evidence					762:769	direct evidence	755:769	direct evidence for LPS and lipid A	755:789	We obtained direct evidence for LPS and lipid A using electrophoresis and differential staining.
26483522	9	77	theme	G.	1439:1440	arg1	reports					1428:1434	previous reports	1419:1434	previous reports of G. obscuriglobus whole-cell fatty acid (FA)	1419:1481	We also confirmed previous reports of G. obscuriglobus whole-cell fatty acid (FA) and sterol compositions and detected a novel polyunsaturated FA (PUFA).
26483522	9	77	theme	G.	1439:1440	arg1	compositions					1494:1505	sterol compositions	1487:1505	sterol compositions	1487:1505	We also confirmed previous reports of G. obscuriglobus whole-cell fatty acid (FA) and sterol compositions and detected a novel polyunsaturated FA (PUFA).
26483522	12	78	theme	IMPORTANCE	1919:1928	arg1	negative					1983:1990	negative	1983:1990	negative	1983:1990	IMPORTANCE Bacterial species are classified as Gram positive or negative based on their cell envelope structure.
26483522	12	78	theme	IMPORTANCE	1919:1928	arg1	positive					1971:1978	positive	1971:1978	positive	1971:1978	IMPORTANCE Bacterial species are classified as Gram positive or negative based on their cell envelope structure.
26483522	12	78	theme	IMPORTANCE	1919:1928	arg1	species					1940:1946	IMPORTANCE Bacterial species	1919:1946	IMPORTANCE Bacterial species	1919:1946	IMPORTANCE Bacterial species are classified as Gram positive or negative based on their cell envelope structure.
26483522	8	79	theme	MS	1256:1257	arg1	analysis					1259:1266	flight (MALDI-TOF) MS analysis	1237:1266	flight (MALDI-TOF) MS analysis of putative intact lipid A	1237:1293	Combined with molecular/structural information collected from matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) MS analysis of putative intact lipid A, these data led us to propose a heterogeneous hexa-acylated lipid A structure (multiple-lipid A species).
26483522	11	80	theme	connections	1760:1770	arg1	studies					1734:1740	studies	1734:1740	studies of the structural connections between planctomycete LPS and peptidoglycans	1734:1815	The pursuit of this question, together with studies of the structural connections between planctomycete LPS and peptidoglycans, will shed more light on what appears to be a planctomycete variation on the Gram-negative cell plan.
26483522	15	81	theme	cell	2553:2556	arg1	envelope					2558:2565	the planctomycete cell envelope	2535:2565	the planctomycete cell envelope	2535:2565	Here, we report direct evidence of lipopolysaccharide in the planctomycete G. obscuriglobus, suggesting the presence of an OM and supporting the proposal that the planctomycete cell envelope is an extension of the canonical Gram-negative plan.
26483522	15	81	theme	cell	2553:2556	arg1	extension					2573:2581	an extension	2570:2581	an extension of the canonical Gram-negative plan	2570:2617	Here, we report direct evidence of lipopolysaccharide in the planctomycete G. obscuriglobus, suggesting the presence of an OM and supporting the proposal that the planctomycete cell envelope is an extension of the canonical Gram-negative plan.
26483522	9	82	theme	whole-cell	1456:1465	arg1	FA					1479:1480	FA	1479:1480	FA	1479:1480	We also confirmed previous reports of G. obscuriglobus whole-cell fatty acid (FA) and sterol compositions and detected a novel polyunsaturated FA (PUFA).
26483522	9	82	theme	whole-cell	1456:1465	arg1	acid					1473:1476	whole-cell fatty acid	1456:1476	G. obscuriglobus whole-cell fatty acid (FA)	1439:1481	We also confirmed previous reports of G. obscuriglobus whole-cell fatty acid (FA) and sterol compositions and detected a novel polyunsaturated FA (PUFA).
26483522	8	83	theme	laser	1201:1205	arg1	ionization-time					1218:1232	matrix-assisted laser desorption ionization-time	1185:1232	matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) MS analysis of putative intact lipid A	1185:1293	Combined with molecular/structural information collected from matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) MS analysis of putative intact lipid A, these data led us to propose a heterogeneous hexa-acylated lipid A structure (multiple-lipid A species).
26483522	15	84	from	evidence	2399:2406	arg1	obscuriglobus					2454:2466	the planctomycete G. obscuriglobus	2433:2466	the planctomycete G. obscuriglobus	2433:2466	Here, we report direct evidence of lipopolysaccharide in the planctomycete G. obscuriglobus, suggesting the presence of an OM and supporting the proposal that the planctomycete cell envelope is an extension of the canonical Gram-negative plan.
26483522	2	85	theme	recent	335:340	arg1	studies					342:348	recent studies	335:348	recent studies	335:348	However, data from recent studies are more consistent with a modified Gram-negative cell plan.
26483522	0	86	theme	Gram-Negative	133:145	arg1	Membrane					153:160	the Classical Gram-Negative Outer Membrane	119:160	the Classical Gram-Negative Outer Membrane	119:160	Global and Targeted Lipid Analysis of Gemmata obscuriglobus Reveals the Presence of Lipopolysaccharide, a Signature of the Classical Gram-Negative Outer Membrane.
26483522	13	87	theme	bacteria	2076:2083	arg1	envelope					2050:2057	the envelope	2046:2057	the envelope of planctomycete bacteria	2046:2083	For 25 years, the envelope of planctomycete bacteria has been considered a unique exception, as it lacks peptidoglycan and an outer membrane (OM).
26483522	4	88	from	OM	586:587	arg1	planctomycetes					592:605	planctomycetes	592:605	planctomycetes	592:605	Despite genomic evidence for an OM in planctomycetes, no biochemical verification has been reported.
26483522	10	89	contain	possess	1675:1681	arg1	planctomycetes					1660:1673	other planctomycetes	1654:1673	other planctomycetes	1654:1673	Our confirmation of LPS, and by implication an OM, in G. obscuriglobus raises the possibility that other planctomycetes possess an OM.
26483522	10	89	contain	possess	1675:1681	arg2	OM					1686:1687	an OM	1683:1687	an OM	1683:1687	Our confirmation of LPS, and by implication an OM, in G. obscuriglobus raises the possibility that other planctomycetes possess an OM.
26483522	16	90	theme	space	2708:2712	arg1	functions					2719:2727	the functions	2715:2727	the functions	2715:2727	This interpretation features a convoluted cytoplasmic membrane and expanded periplasmic space, the functions of which provide an intriguing avenue for future investigation.
26483522	8	91	theme	intact	1280:1285	arg1	A					1293:1293	putative intact lipid A	1271:1293	putative intact lipid A	1271:1293	Combined with molecular/structural information collected from matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) MS analysis of putative intact lipid A, these data led us to propose a heterogeneous hexa-acylated lipid A structure (multiple-lipid A species).
26483522	0	92	theme	Membrane	153:160	arg1	Lipopolysaccharide					84:101	Lipopolysaccharide	84:101	Lipopolysaccharide	84:101	Global and Targeted Lipid Analysis of Gemmata obscuriglobus Reveals the Presence of Lipopolysaccharide, a Signature of the Classical Gram-Negative Outer Membrane.
26483522	0	92	theme	Membrane	153:160	arg1	Signature					106:114	a Signature	104:114	a Signature of the Classical Gram-Negative Outer Membrane	104:160	Global and Targeted Lipid Analysis of Gemmata obscuriglobus Reveals the Presence of Lipopolysaccharide, a Signature of the Classical Gram-Negative Outer Membrane.
26483522	5	93	from	LPS	695:697	arg1	obscuriglobus					728:740	the planctomycete Gemmata obscuriglobus	702:740	the planctomycete Gemmata obscuriglobus	702:740	We attempted to detect and characterize LPS in the planctomycete Gemmata obscuriglobus.
26483522	3	94	theme	signature	534:542	arg1	lipopolysaccharide					504:521	lipopolysaccharide	504:521	lipopolysaccharide (LPS)	504:527	A key feature of the Gram-negative plan is the presence of an outer membrane (OM), for which lipopolysaccharide (LPS) is a signature molecule.
26483522	3	94	theme	signature	534:542	arg1	molecule					544:551	a signature molecule	532:551	a signature molecule	532:551	A key feature of the Gram-negative plan is the presence of an outer membrane (OM), for which lipopolysaccharide (LPS) is a signature molecule.
26483522	15	95	theme	lipopolysaccharide	2411:2428	arg1	evidence					2399:2406	direct evidence	2392:2406	direct evidence of lipopolysaccharide in the planctomycete G. obscuriglobus	2392:2466	Here, we report direct evidence of lipopolysaccharide in the planctomycete G. obscuriglobus, suggesting the presence of an OM and supporting the proposal that the planctomycete cell envelope is an extension of the canonical Gram-negative plan.
26483522	8	96	theme	flight	1237:1242	arg1	analysis					1259:1266	flight (MALDI-TOF) MS analysis	1237:1266	flight (MALDI-TOF) MS analysis of putative intact lipid A	1237:1293	Combined with molecular/structural information collected from matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) MS analysis of putative intact lipid A, these data led us to propose a heterogeneous hexa-acylated lipid A structure (multiple-lipid A species).
26483522	8	97	theme	A	1293:1293	arg1	analysis					1259:1266	flight (MALDI-TOF) MS analysis	1237:1266	flight (MALDI-TOF) MS analysis of putative intact lipid A	1237:1293	Combined with molecular/structural information collected from matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) MS analysis of putative intact lipid A, these data led us to propose a heterogeneous hexa-acylated lipid A structure (multiple-lipid A species).
26483522	11	98	theme	planctomycete	1780:1792	arg1	LPS					1794:1796	planctomycete LPS	1780:1796	planctomycete LPS	1780:1796	The pursuit of this question, together with studies of the structural connections between planctomycete LPS and peptidoglycans, will shed more light on what appears to be a planctomycete variation on the Gram-negative cell plan.
26483522	7	99	theme	3-deoxy-d-manno-octulosonic	991:1017	arg1	Kdo					1025:1027	Kdo	1025:1027	Kdo	1025:1027	Gas chromatography-mass spectrometry (GC-MS) compositional analysis of LPS extracts identified eight different 3-hydroxy fatty acids (3-HOFAs), 2-keto 3-deoxy-d-manno-octulosonic acid (Kdo), glucosamine, and hexose and heptose sugars, a chemical profile unique to Gram-negative LPS.
26483522	7	99	theme	3-deoxy-d-manno-octulosonic	991:1017	arg1	acid					1019:1022	2-keto 3-deoxy-d-manno-octulosonic acid	984:1022	2-keto 3-deoxy-d-manno-octulosonic acid (Kdo)	984:1028	Gas chromatography-mass spectrometry (GC-MS) compositional analysis of LPS extracts identified eight different 3-hydroxy fatty acids (3-HOFAs), 2-keto 3-deoxy-d-manno-octulosonic acid (Kdo), glucosamine, and hexose and heptose sugars, a chemical profile unique to Gram-negative LPS.
26483522	7	99	theme	3-deoxy-d-manno-octulosonic	991:1017	arg1	acids					967:971	3-hydroxy fatty acids	951:971	eight different 3-hydroxy fatty acids (3-HOFAs)	935:981	Gas chromatography-mass spectrometry (GC-MS) compositional analysis of LPS extracts identified eight different 3-hydroxy fatty acids (3-HOFAs), 2-keto 3-deoxy-d-manno-octulosonic acid (Kdo), glucosamine, and hexose and heptose sugars, a chemical profile unique to Gram-negative LPS.
26483522	11	100	theme	question	1710:1717	arg1	pursuit					1694:1700	The pursuit	1690:1700	The pursuit of this question	1690:1717	The pursuit of this question, together with studies of the structural connections between planctomycete LPS and peptidoglycans, will shed more light on what appears to be a planctomycete variation on the Gram-negative cell plan.
26483522	5	101	theme	planctomycete	706:718	arg1	obscuriglobus					728:740	the planctomycete Gemmata obscuriglobus	702:740	the planctomycete Gemmata obscuriglobus	702:740	We attempted to detect and characterize LPS in the planctomycete Gemmata obscuriglobus.
26483522	1	102	theme	unusual	210:216	arg1	properties					227:236	many unusual cellular properties	205:236	many unusual cellular properties	205:236	UNLABELLED Planctomycete bacteria possess many unusual cellular properties, contributing to a cell plan long considered to be unique among the bacteria.
26483522	15	103	theme	Gram-negative	2600:2612	arg1	plan					2614:2617	the canonical Gram-negative plan	2586:2617	the canonical Gram-negative plan	2586:2617	Here, we report direct evidence of lipopolysaccharide in the planctomycete G. obscuriglobus, suggesting the presence of an OM and supporting the proposal that the planctomycete cell envelope is an extension of the canonical Gram-negative plan.
26483522	15	104	theme	plan	2614:2617	arg1	envelope					2558:2565	the planctomycete cell envelope	2535:2565	the planctomycete cell envelope	2535:2565	Here, we report direct evidence of lipopolysaccharide in the planctomycete G. obscuriglobus, suggesting the presence of an OM and supporting the proposal that the planctomycete cell envelope is an extension of the canonical Gram-negative plan.
26483522	15	104	theme	plan	2614:2617	arg1	extension					2573:2581	an extension	2570:2581	an extension of the canonical Gram-negative plan	2570:2617	Here, we report direct evidence of lipopolysaccharide in the planctomycete G. obscuriglobus, suggesting the presence of an OM and supporting the proposal that the planctomycete cell envelope is an extension of the canonical Gram-negative plan.
26483522	12	105	theme	envelope	2012:2019	arg1	structure					2021:2029	their cell envelope structure	2001:2029	their cell envelope structure	2001:2029	IMPORTANCE Bacterial species are classified as Gram positive or negative based on their cell envelope structure.
26483522	15	106	theme	canonical	2590:2598	arg1	plan					2614:2617	the canonical Gram-negative plan	2586:2617	the canonical Gram-negative plan	2586:2617	Here, we report direct evidence of lipopolysaccharide in the planctomycete G. obscuriglobus, suggesting the presence of an OM and supporting the proposal that the planctomycete cell envelope is an extension of the canonical Gram-negative plan.
26483522	9	107	theme	novel	1522:1526	arg1	FA					1544:1545	a novel polyunsaturated FA	1520:1545	a novel polyunsaturated FA (PUFA)	1520:1552	We also confirmed previous reports of G. obscuriglobus whole-cell fatty acid (FA) and sterol compositions and detected a novel polyunsaturated FA (PUFA).
26483522	9	107	theme	novel	1522:1526	arg1	PUFA					1548:1551	PUFA	1548:1551	PUFA	1548:1551	We also confirmed previous reports of G. obscuriglobus whole-cell fatty acid (FA) and sterol compositions and detected a novel polyunsaturated FA (PUFA).
26483522	15	108	from	lipopolysaccharide	2411:2428	arg1	obscuriglobus					2454:2466	the planctomycete G. obscuriglobus	2433:2466	the planctomycete G. obscuriglobus	2433:2466	Here, we report direct evidence of lipopolysaccharide in the planctomycete G. obscuriglobus, suggesting the presence of an OM and supporting the proposal that the planctomycete cell envelope is an extension of the canonical Gram-negative plan.
26483522	7	109	theme	3-hydroxy	951:959	arg1	acid					1019:1022	2-keto 3-deoxy-d-manno-octulosonic acid	984:1022	2-keto 3-deoxy-d-manno-octulosonic acid (Kdo)	984:1028	Gas chromatography-mass spectrometry (GC-MS) compositional analysis of LPS extracts identified eight different 3-hydroxy fatty acids (3-HOFAs), 2-keto 3-deoxy-d-manno-octulosonic acid (Kdo), glucosamine, and hexose and heptose sugars, a chemical profile unique to Gram-negative LPS.
26483522	7	109	theme	3-hydroxy	951:959	arg1	glucosamine					1031:1041	glucosamine	1031:1041	glucosamine	1031:1041	Gas chromatography-mass spectrometry (GC-MS) compositional analysis of LPS extracts identified eight different 3-hydroxy fatty acids (3-HOFAs), 2-keto 3-deoxy-d-manno-octulosonic acid (Kdo), glucosamine, and hexose and heptose sugars, a chemical profile unique to Gram-negative LPS.
26483522	7	109	theme	3-hydroxy	951:959	arg1	hexose					1048:1053	hexose	1048:1053	hexose	1048:1053	Gas chromatography-mass spectrometry (GC-MS) compositional analysis of LPS extracts identified eight different 3-hydroxy fatty acids (3-HOFAs), 2-keto 3-deoxy-d-manno-octulosonic acid (Kdo), glucosamine, and hexose and heptose sugars, a chemical profile unique to Gram-negative LPS.
26483522	7	109	theme	3-hydroxy	951:959	arg1	3-HOFAs					974:980	3-HOFAs	974:980	3-HOFAs	974:980	Gas chromatography-mass spectrometry (GC-MS) compositional analysis of LPS extracts identified eight different 3-hydroxy fatty acids (3-HOFAs), 2-keto 3-deoxy-d-manno-octulosonic acid (Kdo), glucosamine, and hexose and heptose sugars, a chemical profile unique to Gram-negative LPS.
26483522	7	109	theme	3-hydroxy	951:959	arg1	acids					967:971	3-hydroxy fatty acids	951:971	eight different 3-hydroxy fatty acids (3-HOFAs)	935:981	Gas chromatography-mass spectrometry (GC-MS) compositional analysis of LPS extracts identified eight different 3-hydroxy fatty acids (3-HOFAs), 2-keto 3-deoxy-d-manno-octulosonic acid (Kdo), glucosamine, and hexose and heptose sugars, a chemical profile unique to Gram-negative LPS.
26483522	8	110	theme	hexa-acylated	1341:1353	arg1	structure					1363:1371	a heterogeneous hexa-acylated lipid A structure	1325:1371	a heterogeneous hexa-acylated lipid A structure (multiple-lipid A species)	1325:1398	Combined with molecular/structural information collected from matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) MS analysis of putative intact lipid A, these data led us to propose a heterogeneous hexa-acylated lipid A structure (multiple-lipid A species).
26483522	8	110	theme	hexa-acylated	1341:1353	arg1	species					1391:1397	multiple-lipid A species	1374:1397	multiple-lipid A species	1374:1397	Combined with molecular/structural information collected from matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) MS analysis of putative intact lipid A, these data led us to propose a heterogeneous hexa-acylated lipid A structure (multiple-lipid A species).
26483522	14	111	theme	recent	2197:2202	arg1	detection					2204:2212	the very recent detection	2188:2212	the very recent detection of peptidoglycan in planctomycete species	2188:2254	However, the very recent detection of peptidoglycan in planctomycete species has provided evidence for a more conventional cell wall and raised questions about other elements of the cell envelope.
26483522	2	112	theme	Gram-negative	386:398	arg1	plan					405:408	a modified Gram-negative cell plan	375:408	a modified Gram-negative cell plan	375:408	However, data from recent studies are more consistent with a modified Gram-negative cell plan.
26483522	15	113	theme	G.	2451:2452	arg1	obscuriglobus					2454:2466	the planctomycete G. obscuriglobus	2433:2466	the planctomycete G. obscuriglobus	2433:2466	Here, we report direct evidence of lipopolysaccharide in the planctomycete G. obscuriglobus, suggesting the presence of an OM and supporting the proposal that the planctomycete cell envelope is an extension of the canonical Gram-negative plan.
26483522	16	114	theme	cytoplasmic	2662:2672	arg1	membrane					2674:2681	a convoluted cytoplasmic membrane	2649:2681	a convoluted cytoplasmic membrane	2649:2681	This interpretation features a convoluted cytoplasmic membrane and expanded periplasmic space, the functions of which provide an intriguing avenue for future investigation.
26483522	8	115	theme	A	1361:1361	arg1	structure					1363:1371	a heterogeneous hexa-acylated lipid A structure	1325:1371	a heterogeneous hexa-acylated lipid A structure (multiple-lipid A species)	1325:1398	Combined with molecular/structural information collected from matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) MS analysis of putative intact lipid A, these data led us to propose a heterogeneous hexa-acylated lipid A structure (multiple-lipid A species).
26483522	8	115	theme	A	1361:1361	arg1	species					1391:1397	multiple-lipid A species	1374:1397	multiple-lipid A species	1374:1397	Combined with molecular/structural information collected from matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) MS analysis of putative intact lipid A, these data led us to propose a heterogeneous hexa-acylated lipid A structure (multiple-lipid A species).
26483522	0	116	theme	Lipid	20:24	arg1	Analysis					26:33	Global and Targeted Lipid Analysis	0:33	Global and Targeted Lipid Analysis of Gemmata obscuriglobus	0:58	Global and Targeted Lipid Analysis of Gemmata obscuriglobus Reveals the Presence of Lipopolysaccharide, a Signature of the Classical Gram-Negative Outer Membrane.
26483522	7	117	theme	extracts	915:922	arg1	analysis					899:906	Gas chromatography-mass spectrometry (GC-MS) compositional analysis	840:906	Gas chromatography-mass spectrometry (GC-MS) compositional analysis of LPS extracts	840:922	Gas chromatography-mass spectrometry (GC-MS) compositional analysis of LPS extracts identified eight different 3-hydroxy fatty acids (3-HOFAs), 2-keto 3-deoxy-d-manno-octulosonic acid (Kdo), glucosamine, and hexose and heptose sugars, a chemical profile unique to Gram-negative LPS.
26483522	16	118	theme	periplasmic	2696:2706	arg1	space					2708:2712	periplasmic space	2696:2712	periplasmic space	2696:2712	This interpretation features a convoluted cytoplasmic membrane and expanded periplasmic space, the functions of which provide an intriguing avenue for future investigation.
26483522	3	119	theme	key	413:415	arg1	feature					417:423	A key feature	411:423	A key feature of the Gram-negative plan	411:449	A key feature of the Gram-negative plan is the presence of an outer membrane (OM), for which lipopolysaccharide (LPS) is a signature molecule.
26483522	3	119	theme	key	413:415	arg1	presence					458:465	the presence	454:465	the presence of an outer membrane (OM), for which lipopolysaccharide (LPS) is a signature molecule	454:551	A key feature of the Gram-negative plan is the presence of an outer membrane (OM), for which lipopolysaccharide (LPS) is a signature molecule.
26483522	8	120	theme	A	1389:1389	arg1	structure					1363:1371	a heterogeneous hexa-acylated lipid A structure	1325:1371	a heterogeneous hexa-acylated lipid A structure (multiple-lipid A species)	1325:1398	Combined with molecular/structural information collected from matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) MS analysis of putative intact lipid A, these data led us to propose a heterogeneous hexa-acylated lipid A structure (multiple-lipid A species).
26483522	8	120	theme	A	1389:1389	arg1	species					1391:1397	multiple-lipid A species	1374:1397	multiple-lipid A species	1374:1397	Combined with molecular/structural information collected from matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) MS analysis of putative intact lipid A, these data led us to propose a heterogeneous hexa-acylated lipid A structure (multiple-lipid A species).
26483522	7	121	theme	Gram-negative	1104:1116	arg1	LPS					1118:1120	Gram-negative LPS	1104:1120	Gram-negative LPS	1104:1120	Gas chromatography-mass spectrometry (GC-MS) compositional analysis of LPS extracts identified eight different 3-hydroxy fatty acids (3-HOFAs), 2-keto 3-deoxy-d-manno-octulosonic acid (Kdo), glucosamine, and hexose and heptose sugars, a chemical profile unique to Gram-negative LPS.
26483522	6	122	theme	differential	817:828	arg1	staining					830:837	differential staining	817:837	differential staining	817:837	We obtained direct evidence for LPS and lipid A using electrophoresis and differential staining.
26483522	10	123	from	OM	1602:1603	arg1	obscuriglobus					1612:1624	G. obscuriglobus	1609:1624	G. obscuriglobus	1609:1624	Our confirmation of LPS, and by implication an OM, in G. obscuriglobus raises the possibility that other planctomycetes possess an OM.
26483522	14	124	theme	cell	2361:2364	arg1	envelope					2366:2373	the cell envelope	2357:2373	the cell envelope	2357:2373	However, the very recent detection of peptidoglycan in planctomycete species has provided evidence for a more conventional cell wall and raised questions about other elements of the cell envelope.
26483522	3	125	theme	membrane	479:486	arg1	feature					417:423	A key feature	411:423	A key feature of the Gram-negative plan	411:449	A key feature of the Gram-negative plan is the presence of an outer membrane (OM), for which lipopolysaccharide (LPS) is a signature molecule.
26483522	3	125	theme	membrane	479:486	arg1	presence					458:465	the presence	454:465	the presence of an outer membrane (OM), for which lipopolysaccharide (LPS) is a signature molecule	454:551	A key feature of the Gram-negative plan is the presence of an outer membrane (OM), for which lipopolysaccharide (LPS) is a signature molecule.
26483522	13	126	theme	outer	2158:2162	arg1	OM					2174:2175	OM	2174:2175	OM	2174:2175	For 25 years, the envelope of planctomycete bacteria has been considered a unique exception, as it lacks peptidoglycan and an outer membrane (OM).
26483522	13	126	theme	outer	2158:2162	arg1	membrane					2164:2171	an outer membrane	2155:2171	an outer membrane (OM)	2155:2176	For 25 years, the envelope of planctomycete bacteria has been considered a unique exception, as it lacks peptidoglycan and an outer membrane (OM).
26483522	2	127	with	consistent	359:368	arg1	plan					405:408	a modified Gram-negative cell plan	375:408	a modified Gram-negative cell plan	375:408	However, data from recent studies are more consistent with a modified Gram-negative cell plan.
26483522	3	128	theme	Gram-negative	432:444	arg1	plan					446:449	the Gram-negative plan	428:449	the Gram-negative plan	428:449	A key feature of the Gram-negative plan is the presence of an outer membrane (OM), for which lipopolysaccharide (LPS) is a signature molecule.
26483522	14	129	theme	other	2339:2343	arg1	elements					2345:2352	other elements	2339:2352	other elements of the cell envelope	2339:2373	However, the very recent detection of peptidoglycan in planctomycete species has provided evidence for a more conventional cell wall and raised questions about other elements of the cell envelope.
26483522	1	130	theme	cell	257:260	arg1	plan					262:265	a cell plan	255:265	a cell plan long considered to be unique among the bacteria	255:313	UNLABELLED Planctomycete bacteria possess many unusual cellular properties, contributing to a cell plan long considered to be unique among the bacteria.
28275152	3	0	theme	chemistry	558:566	arg1	strategy					568:575	a medicinal chemistry strategy	546:575	a medicinal chemistry strategy based on a combination of automated glycan assembly (AGA), glycan microarray-based monoclonal antibody (mAb) reverse engineering, and immunological evaluation in vivo	546:742	We describe a medicinal chemistry strategy based on a combination of automated glycan assembly (AGA), glycan microarray-based monoclonal antibody (mAb) reverse engineering, and immunological evaluation in vivo to uncover a protective glycan epitope (glycotope) for S. pneumoniae serotype 8 (ST8).
28275152	8	1	theme	14-valent	1583:1591	arg1	vaccine					1607:1613	a potent 14-valent S. pneumoniae vaccine	1574:1613	a potent 14-valent S. pneumoniae vaccine	1574:1613	Coformulation of ST8 glycoconjugates with the marketed 13-valent glycoconjugate vaccine Prevnar 13 yielded a potent 14-valent S. pneumoniae vaccine.
28275152	7	2	with	Immunization	1329:1340	arg1	glycoconjugates					1376:1390	semisynthetic ST8 glycoconjugates	1358:1390	semisynthetic ST8 glycoconjugates containing protective glycotopes	1358:1423	Immunization of rabbits with semisynthetic ST8 glycoconjugates containing protective glycotopes induced an antibacterial immune response.
28275152	1	3	theme	Streptococcus	172:184	arg1	pneumoniae					186:195	Streptococcus pneumoniae	172:195	Streptococcus pneumoniae	172:195	Glycoconjugate vaccines based on capsular polysaccharides (CPSs) of pathogenic bacteria such as Streptococcus pneumoniae successfully protect from disease but suffer from incomplete coverage, are troublesome to manufacture from isolated CPSs, and lack efficacy against certain serotypes.
28275152	9	4	theme	glycoconjugate	1691:1704	arg1	vaccines					1706:1713	efficient semisynthetic glycoconjugate vaccines	1667:1713	efficient semisynthetic glycoconjugate vaccines	1667:1713	Our strategy presents a facile approach to develop efficient semisynthetic glycoconjugate vaccines.
28275152	7	5	theme	immune	1450:1455	arg1	response					1457:1464	an antibacterial immune response	1433:1464	an antibacterial immune response	1433:1464	Immunization of rabbits with semisynthetic ST8 glycoconjugates containing protective glycotopes induced an antibacterial immune response.
28275152	4	6	used	used	904:907	arg2	frameshifts					856:866	All four tetrasaccharide frameshifts	831:866	All four tetrasaccharide frameshifts of ST8 CPS	831:877	All four tetrasaccharide frameshifts of ST8 CPS were prepared by AGA and used in glycan microarray experiments to identify the glycotopes recognized by antibodies against ST8.
28275152	3	7	theme	assembly	620:627	arg1	combination					588:598	a combination	586:598	a combination of automated glycan assembly (AGA), glycan microarray-based monoclonal antibody (mAb) reverse engineering, and immunological evaluation	586:734	We describe a medicinal chemistry strategy based on a combination of automated glycan assembly (AGA), glycan microarray-based monoclonal antibody (mAb) reverse engineering, and immunological evaluation in vivo to uncover a protective glycan epitope (glycotope) for S. pneumoniae serotype 8 (ST8).
28275152	5	8	theme	protein	1137:1143	arg1	CRM197					1145:1150	the carrier protein CRM197	1125:1150	the carrier protein CRM197	1125:1150	One tetrasaccharide frameshift that was preferentially recognized by a protective, CPS-directed mAb was conjugated to the carrier protein CRM197.
28275152	3	9	theme	automated	603:611	arg1	assembly					620:627	automated glycan assembly	603:627	automated glycan assembly (AGA)	603:633	We describe a medicinal chemistry strategy based on a combination of automated glycan assembly (AGA), glycan microarray-based monoclonal antibody (mAb) reverse engineering, and immunological evaluation in vivo to uncover a protective glycan epitope (glycotope) for S. pneumoniae serotype 8 (ST8).
28275152	3	9	theme	automated	603:611	arg1	AGA					630:632	AGA	630:632	AGA	630:632	We describe a medicinal chemistry strategy based on a combination of automated glycan assembly (AGA), glycan microarray-based monoclonal antibody (mAb) reverse engineering, and immunological evaluation in vivo to uncover a protective glycan epitope (glycotope) for S. pneumoniae serotype 8 (ST8).
28275152	1	10	theme	Glycoconjugate	76:89	arg1	vaccines					91:98	Glycoconjugate vaccines	76:98	Glycoconjugate vaccines based on capsular polysaccharides (CPSs) of pathogenic bacteria such as Streptococcus pneumoniae	76:195	Glycoconjugate vaccines based on capsular polysaccharides (CPSs) of pathogenic bacteria such as Streptococcus pneumoniae successfully protect from disease but suffer from incomplete coverage, are troublesome to manufacture from isolated CPSs, and lack efficacy against certain serotypes.
28275152	7	11	theme	semisynthetic	1358:1370	arg1	glycoconjugates					1376:1390	semisynthetic ST8 glycoconjugates	1358:1390	semisynthetic ST8 glycoconjugates containing protective glycotopes	1358:1423	Immunization of rabbits with semisynthetic ST8 glycoconjugates containing protective glycotopes induced an antibacterial immune response.
28275152	9	12	theme	efficient	1667:1675	arg1	vaccines					1706:1713	efficient semisynthetic glycoconjugate vaccines	1667:1713	efficient semisynthetic glycoconjugate vaccines	1667:1713	Our strategy presents a facile approach to develop efficient semisynthetic glycoconjugate vaccines.
28275152	4	13	theme	CPS	875:877	arg1	frameshifts					856:866	All four tetrasaccharide frameshifts	831:866	All four tetrasaccharide frameshifts of ST8 CPS	831:877	All four tetrasaccharide frameshifts of ST8 CPS were prepared by AGA and used in glycan microarray experiments to identify the glycotopes recognized by antibodies against ST8.
28275152	8	14	dep	vaccine	1547:1553	arg1	Prevnar					1555:1561	Prevnar	1555:1561	Prevnar	1555:1561	Coformulation of ST8 glycoconjugates with the marketed 13-valent glycoconjugate vaccine Prevnar 13 yielded a potent 14-valent S. pneumoniae vaccine.
28275152	8	15	theme	ST8	1484:1486	arg1	glycoconjugates					1488:1502	ST8 glycoconjugates	1484:1502	ST8 glycoconjugates with the marketed 13-valent glycoconjugate vaccine Prevnar 13	1484:1564	Coformulation of ST8 glycoconjugates with the marketed 13-valent glycoconjugate vaccine Prevnar 13 yielded a potent 14-valent S. pneumoniae vaccine.
28275152	2	16	theme	isolated	433:440	arg1	CPSs					442:445	isolated CPSs	433:445	isolated CPSs	433:445	Defined, synthetic oligosaccharides are an attractive alternative to isolated CPSs but require the identification of immunogenic and protective oligosaccharide antigens.
28275152	8	17	with	glycoconjugates	1488:1502	arg1	vaccine					1547:1553	the marketed 13-valent glycoconjugate vaccine Prevnar 13	1509:1564	the marketed 13-valent glycoconjugate vaccine Prevnar 13	1509:1564	Coformulation of ST8 glycoconjugates with the marketed 13-valent glycoconjugate vaccine Prevnar 13 yielded a potent 14-valent S. pneumoniae vaccine.
28275152	3	18	theme	protective	757:766	arg1	glycotope					784:792	glycotope	784:792	glycotope	784:792	We describe a medicinal chemistry strategy based on a combination of automated glycan assembly (AGA), glycan microarray-based monoclonal antibody (mAb) reverse engineering, and immunological evaluation in vivo to uncover a protective glycan epitope (glycotope) for S. pneumoniae serotype 8 (ST8).
28275152	3	18	theme	protective	757:766	arg1	epitope					775:781	a protective glycan epitope	755:781	a protective glycan epitope (glycotope) for S. pneumoniae serotype 8 (ST8)	755:828	We describe a medicinal chemistry strategy based on a combination of automated glycan assembly (AGA), glycan microarray-based monoclonal antibody (mAb) reverse engineering, and immunological evaluation in vivo to uncover a protective glycan epitope (glycotope) for S. pneumoniae serotype 8 (ST8).
28275152	4	19	theme	ST8	871:873	arg1	CPS					875:877	ST8 CPS	871:877	ST8 CPS	871:877	All four tetrasaccharide frameshifts of ST8 CPS were prepared by AGA and used in glycan microarray experiments to identify the glycotopes recognized by antibodies against ST8.
28275152	5	20	theme	tetrasaccharide	1011:1025	arg1	frameshift					1027:1036	One tetrasaccharide frameshift	1007:1036	One tetrasaccharide frameshift that was preferentially recognized by a protective, CPS-directed mAb	1007:1105	One tetrasaccharide frameshift that was preferentially recognized by a protective, CPS-directed mAb was conjugated to the carrier protein CRM197.
28275152	8	21	theme	glycoconjugate	1532:1545	arg1	vaccine					1547:1553	the marketed 13-valent glycoconjugate vaccine Prevnar 13	1509:1564	the marketed 13-valent glycoconjugate vaccine Prevnar 13	1509:1564	Coformulation of ST8 glycoconjugates with the marketed 13-valent glycoconjugate vaccine Prevnar 13 yielded a potent 14-valent S. pneumoniae vaccine.
28275152	5	22	dep	protective	1078:1087	arg1	CPS-directed					1090:1101	CPS-directed	1090:1101	CPS-directed	1090:1101	One tetrasaccharide frameshift that was preferentially recognized by a protective, CPS-directed mAb was conjugated to the carrier protein CRM197.
28275152	0	23	theme	semisynthetic	2:14	arg1	serotype					41:48	A semisynthetic Streptococcus pneumoniae serotype 8	0:50	A semisynthetic Streptococcus pneumoniae serotype 8	0:50	A semisynthetic Streptococcus pneumoniae serotype 8 glycoconjugate vaccine.
28275152	3	24	theme	glycan	768:773	arg1	glycotope					784:792	glycotope	784:792	glycotope	784:792	We describe a medicinal chemistry strategy based on a combination of automated glycan assembly (AGA), glycan microarray-based monoclonal antibody (mAb) reverse engineering, and immunological evaluation in vivo to uncover a protective glycan epitope (glycotope) for S. pneumoniae serotype 8 (ST8).
28275152	3	24	theme	glycan	768:773	arg1	epitope					775:781	a protective glycan epitope	755:781	a protective glycan epitope (glycotope) for S. pneumoniae serotype 8 (ST8)	755:828	We describe a medicinal chemistry strategy based on a combination of automated glycan assembly (AGA), glycan microarray-based monoclonal antibody (mAb) reverse engineering, and immunological evaluation in vivo to uncover a protective glycan epitope (glycotope) for S. pneumoniae serotype 8 (ST8).
28275152	8	25	theme	13-valent	1522:1530	arg1	vaccine					1547:1553	the marketed 13-valent glycoconjugate vaccine Prevnar 13	1509:1564	the marketed 13-valent glycoconjugate vaccine Prevnar 13	1509:1564	Coformulation of ST8 glycoconjugates with the marketed 13-valent glycoconjugate vaccine Prevnar 13 yielded a potent 14-valent S. pneumoniae vaccine.
28275152	9	26	theme	facile	1640:1645	arg1	approach					1647:1654	a facile approach	1638:1654	a facile approach to develop efficient semisynthetic glycoconjugate vaccines	1638:1713	Our strategy presents a facile approach to develop efficient semisynthetic glycoconjugate vaccines.
28275152	2	27	theme	immunogenic	481:491	arg1	antigens					524:531	immunogenic and protective oligosaccharide antigens	481:531	immunogenic and protective oligosaccharide antigens	481:531	Defined, synthetic oligosaccharides are an attractive alternative to isolated CPSs but require the identification of immunogenic and protective oligosaccharide antigens.
28275152	1	28	theme	isolated	304:311	arg1	CPSs					313:316	isolated CPSs	304:316	isolated CPSs	304:316	Glycoconjugate vaccines based on capsular polysaccharides (CPSs) of pathogenic bacteria such as Streptococcus pneumoniae successfully protect from disease but suffer from incomplete coverage, are troublesome to manufacture from isolated CPSs, and lack efficacy against certain serotypes.
28275152	1	29	theme	capsular	109:116	arg1	CPSs					135:138	CPSs	135:138	CPSs	135:138	Glycoconjugate vaccines based on capsular polysaccharides (CPSs) of pathogenic bacteria such as Streptococcus pneumoniae successfully protect from disease but suffer from incomplete coverage, are troublesome to manufacture from isolated CPSs, and lack efficacy against certain serotypes.
28275152	1	29	theme	capsular	109:116	arg1	polysaccharides					118:132	capsular polysaccharides	109:132	capsular polysaccharides (CPSs) of pathogenic bacteria such as Streptococcus pneumoniae	109:195	Glycoconjugate vaccines based on capsular polysaccharides (CPSs) of pathogenic bacteria such as Streptococcus pneumoniae successfully protect from disease but suffer from incomplete coverage, are troublesome to manufacture from isolated CPSs, and lack efficacy against certain serotypes.
28275152	2	30	theme	attractive	407:416	arg1	oligosaccharides					383:398	Defined, synthetic oligosaccharides	364:398	Defined, synthetic oligosaccharides	364:398	Defined, synthetic oligosaccharides are an attractive alternative to isolated CPSs but require the identification of immunogenic and protective oligosaccharide antigens.
28275152	2	30	theme	attractive	407:416	arg1	alternative					418:428	an attractive alternative	404:428	an attractive alternative to isolated CPSs	404:445	Defined, synthetic oligosaccharides are an attractive alternative to isolated CPSs but require the identification of immunogenic and protective oligosaccharide antigens.
28275152	0	31	theme	pneumoniae	30:39	arg1	serotype					41:48	A semisynthetic Streptococcus pneumoniae serotype 8	0:50	A semisynthetic Streptococcus pneumoniae serotype 8	0:50	A semisynthetic Streptococcus pneumoniae serotype 8 glycoconjugate vaccine.
28275152	3	32	theme	S.	799:800	arg1	serotype					813:820	S. pneumoniae serotype 8 (ST8)	799:828	S. pneumoniae serotype 8 (ST8)	799:828	We describe a medicinal chemistry strategy based on a combination of automated glycan assembly (AGA), glycan microarray-based monoclonal antibody (mAb) reverse engineering, and immunological evaluation in vivo to uncover a protective glycan epitope (glycotope) for S. pneumoniae serotype 8 (ST8).
28275152	5	33	theme	carrier	1129:1135	arg1	CRM197					1145:1150	the carrier protein CRM197	1125:1150	the carrier protein CRM197	1125:1150	One tetrasaccharide frameshift that was preferentially recognized by a protective, CPS-directed mAb was conjugated to the carrier protein CRM197.
28275152	6	34	theme	protective	1288:1297	arg1	glycotopes					1317:1326	protective and nonprotective glycotopes	1288:1326	protective and nonprotective glycotopes	1288:1326	Immunization of mice with this semisynthetic glycoconjugate followed by generation and characterization of a protective mAb identified protective and nonprotective glycotopes.
28275152	3	35	theme	glycan	613:618	arg1	assembly					620:627	automated glycan assembly	603:627	automated glycan assembly (AGA)	603:633	We describe a medicinal chemistry strategy based on a combination of automated glycan assembly (AGA), glycan microarray-based monoclonal antibody (mAb) reverse engineering, and immunological evaluation in vivo to uncover a protective glycan epitope (glycotope) for S. pneumoniae serotype 8 (ST8).
28275152	3	35	theme	glycan	613:618	arg1	AGA					630:632	AGA	630:632	AGA	630:632	We describe a medicinal chemistry strategy based on a combination of automated glycan assembly (AGA), glycan microarray-based monoclonal antibody (mAb) reverse engineering, and immunological evaluation in vivo to uncover a protective glycan epitope (glycotope) for S. pneumoniae serotype 8 (ST8).
28275152	4	36	theme	tetrasaccharide	840:854	arg1	frameshifts					856:866	All four tetrasaccharide frameshifts	831:866	All four tetrasaccharide frameshifts of ST8 CPS	831:877	All four tetrasaccharide frameshifts of ST8 CPS were prepared by AGA and used in glycan microarray experiments to identify the glycotopes recognized by antibodies against ST8.
28275152	0	37	theme	Streptococcus	16:28	arg1	serotype					41:48	A semisynthetic Streptococcus pneumoniae serotype 8	0:50	A semisynthetic Streptococcus pneumoniae serotype 8	0:50	A semisynthetic Streptococcus pneumoniae serotype 8 glycoconjugate vaccine.
28275152	3	38	theme	engineering	694:704	arg1	combination					588:598	a combination	586:598	a combination of automated glycan assembly (AGA), glycan microarray-based monoclonal antibody (mAb) reverse engineering, and immunological evaluation	586:734	We describe a medicinal chemistry strategy based on a combination of automated glycan assembly (AGA), glycan microarray-based monoclonal antibody (mAb) reverse engineering, and immunological evaluation in vivo to uncover a protective glycan epitope (glycotope) for S. pneumoniae serotype 8 (ST8).
28275152	2	39	dep	Defined	364:370	arg1	synthetic					373:381	synthetic	373:381	synthetic	373:381	Defined, synthetic oligosaccharides are an attractive alternative to isolated CPSs but require the identification of immunogenic and protective oligosaccharide antigens.
28275152	6	40	theme	protective	1262:1271	arg1	mAb					1273:1275	a protective mAb	1260:1275	a protective mAb	1260:1275	Immunization of mice with this semisynthetic glycoconjugate followed by generation and characterization of a protective mAb identified protective and nonprotective glycotopes.
28275152	8	41	theme	potent	1576:1581	arg1	vaccine					1607:1613	a potent 14-valent S. pneumoniae vaccine	1574:1613	a potent 14-valent S. pneumoniae vaccine	1574:1613	Coformulation of ST8 glycoconjugates with the marketed 13-valent glycoconjugate vaccine Prevnar 13 yielded a potent 14-valent S. pneumoniae vaccine.
28275152	3	42	theme	medicinal	548:556	arg1	strategy					568:575	a medicinal chemistry strategy	546:575	a medicinal chemistry strategy based on a combination of automated glycan assembly (AGA), glycan microarray-based monoclonal antibody (mAb) reverse engineering, and immunological evaluation in vivo	546:742	We describe a medicinal chemistry strategy based on a combination of automated glycan assembly (AGA), glycan microarray-based monoclonal antibody (mAb) reverse engineering, and immunological evaluation in vivo to uncover a protective glycan epitope (glycotope) for S. pneumoniae serotype 8 (ST8).
28275152	8	43	dep	S.	1593:1594	arg1	pneumoniae					1596:1605	pneumoniae	1596:1605	pneumoniae	1596:1605	Coformulation of ST8 glycoconjugates with the marketed 13-valent glycoconjugate vaccine Prevnar 13 yielded a potent 14-valent S. pneumoniae vaccine.
28275152	7	44	contain	containing	1392:1401	arg2	glycotopes					1414:1423	protective glycotopes	1403:1423	protective glycotopes	1403:1423	Immunization of rabbits with semisynthetic ST8 glycoconjugates containing protective glycotopes induced an antibacterial immune response.
28275152	7	44	contain	containing	1392:1401	arg1	glycoconjugates					1376:1390	semisynthetic ST8 glycoconjugates	1358:1390	semisynthetic ST8 glycoconjugates containing protective glycotopes	1358:1423	Immunization of rabbits with semisynthetic ST8 glycoconjugates containing protective glycotopes induced an antibacterial immune response.
28275152	3	45	theme	glycan	636:641	arg1	engineering					694:704	glycan microarray-based monoclonal antibody (mAb) reverse engineering	636:704	glycan microarray-based monoclonal antibody (mAb) reverse engineering	636:704	We describe a medicinal chemistry strategy based on a combination of automated glycan assembly (AGA), glycan microarray-based monoclonal antibody (mAb) reverse engineering, and immunological evaluation in vivo to uncover a protective glycan epitope (glycotope) for S. pneumoniae serotype 8 (ST8).
28275152	7	46	theme	antibacterial	1436:1448	arg1	response					1457:1464	an antibacterial immune response	1433:1464	an antibacterial immune response	1433:1464	Immunization of rabbits with semisynthetic ST8 glycoconjugates containing protective glycotopes induced an antibacterial immune response.
28275152	9	47	theme	semisynthetic	1677:1689	arg1	vaccines					1706:1713	efficient semisynthetic glycoconjugate vaccines	1667:1713	efficient semisynthetic glycoconjugate vaccines	1667:1713	Our strategy presents a facile approach to develop efficient semisynthetic glycoconjugate vaccines.
28275152	2	48	theme	antigens	524:531	arg1	identification					463:476	the identification	459:476	the identification of immunogenic and protective oligosaccharide antigens	459:531	Defined, synthetic oligosaccharides are an attractive alternative to isolated CPSs but require the identification of immunogenic and protective oligosaccharide antigens.
28275152	3	49	theme	microarray-based	643:658	arg1	antibody					671:678	microarray-based monoclonal antibody	643:678	glycan microarray-based monoclonal antibody (mAb) reverse engineering	636:704	We describe a medicinal chemistry strategy based on a combination of automated glycan assembly (AGA), glycan microarray-based monoclonal antibody (mAb) reverse engineering, and immunological evaluation in vivo to uncover a protective glycan epitope (glycotope) for S. pneumoniae serotype 8 (ST8).
28275152	3	49	theme	microarray-based	643:658	arg1	mAb					681:683	mAb	681:683	mAb	681:683	We describe a medicinal chemistry strategy based on a combination of automated glycan assembly (AGA), glycan microarray-based monoclonal antibody (mAb) reverse engineering, and immunological evaluation in vivo to uncover a protective glycan epitope (glycotope) for S. pneumoniae serotype 8 (ST8).
28275152	7	50	theme	protective	1403:1412	arg1	glycotopes					1414:1423	protective glycotopes	1403:1423	protective glycotopes	1403:1423	Immunization of rabbits with semisynthetic ST8 glycoconjugates containing protective glycotopes induced an antibacterial immune response.
28275152	2	51	theme	oligosaccharide	508:522	arg1	antigens					524:531	immunogenic and protective oligosaccharide antigens	481:531	immunogenic and protective oligosaccharide antigens	481:531	Defined, synthetic oligosaccharides are an attractive alternative to isolated CPSs but require the identification of immunogenic and protective oligosaccharide antigens.
28275152	4	52	theme	glycan	912:917	arg1	experiments					930:940	glycan microarray experiments	912:940	glycan microarray experiments	912:940	All four tetrasaccharide frameshifts of ST8 CPS were prepared by AGA and used in glycan microarray experiments to identify the glycotopes recognized by antibodies against ST8.
28275152	3	53	theme	reverse	686:692	arg1	engineering					694:704	glycan microarray-based monoclonal antibody (mAb) reverse engineering	636:704	glycan microarray-based monoclonal antibody (mAb) reverse engineering	636:704	We describe a medicinal chemistry strategy based on a combination of automated glycan assembly (AGA), glycan microarray-based monoclonal antibody (mAb) reverse engineering, and immunological evaluation in vivo to uncover a protective glycan epitope (glycotope) for S. pneumoniae serotype 8 (ST8).
28275152	6	54	with	Immunization	1153:1164	arg1	glycoconjugate					1198:1211	this semisynthetic glycoconjugate	1179:1211	this semisynthetic glycoconjugate followed by generation and characterization of a protective mAb	1179:1275	Immunization of mice with this semisynthetic glycoconjugate followed by generation and characterization of a protective mAb identified protective and nonprotective glycotopes.
28275152	7	55	theme	ST8	1372:1374	arg1	glycoconjugates					1376:1390	semisynthetic ST8 glycoconjugates	1358:1390	semisynthetic ST8 glycoconjugates containing protective glycotopes	1358:1423	Immunization of rabbits with semisynthetic ST8 glycoconjugates containing protective glycotopes induced an antibacterial immune response.
28275152	2	56	theme	protective	497:506	arg1	antigens					524:531	immunogenic and protective oligosaccharide antigens	481:531	immunogenic and protective oligosaccharide antigens	481:531	Defined, synthetic oligosaccharides are an attractive alternative to isolated CPSs but require the identification of immunogenic and protective oligosaccharide antigens.
28275152	3	57	theme	pneumoniae	802:811	arg1	serotype					813:820	S. pneumoniae serotype 8 (ST8)	799:828	S. pneumoniae serotype 8 (ST8)	799:828	We describe a medicinal chemistry strategy based on a combination of automated glycan assembly (AGA), glycan microarray-based monoclonal antibody (mAb) reverse engineering, and immunological evaluation in vivo to uncover a protective glycan epitope (glycotope) for S. pneumoniae serotype 8 (ST8).
28275152	8	58	dep	14-valent	1583:1591	arg1	S.					1593:1594	S.	1593:1594	S.	1593:1594	Coformulation of ST8 glycoconjugates with the marketed 13-valent glycoconjugate vaccine Prevnar 13 yielded a potent 14-valent S. pneumoniae vaccine.
28275152	3	59	theme	evaluation	725:734	arg1	combination					588:598	a combination	586:598	a combination of automated glycan assembly (AGA), glycan microarray-based monoclonal antibody (mAb) reverse engineering, and immunological evaluation	586:734	We describe a medicinal chemistry strategy based on a combination of automated glycan assembly (AGA), glycan microarray-based monoclonal antibody (mAb) reverse engineering, and immunological evaluation in vivo to uncover a protective glycan epitope (glycotope) for S. pneumoniae serotype 8 (ST8).
28275152	5	60	theme	protective	1078:1087	arg1	mAb					1103:1105	a protective, CPS-directed mAb	1076:1105	a protective, CPS-directed mAb	1076:1105	One tetrasaccharide frameshift that was preferentially recognized by a protective, CPS-directed mAb was conjugated to the carrier protein CRM197.
28275152	2	61	theme	Defined	364:370	arg1	oligosaccharides					383:398	Defined, synthetic oligosaccharides	364:398	Defined, synthetic oligosaccharides	364:398	Defined, synthetic oligosaccharides are an attractive alternative to isolated CPSs but require the identification of immunogenic and protective oligosaccharide antigens.
28275152	2	61	theme	Defined	364:370	arg1	alternative					418:428	an attractive alternative	404:428	an attractive alternative to isolated CPSs	404:445	Defined, synthetic oligosaccharides are an attractive alternative to isolated CPSs but require the identification of immunogenic and protective oligosaccharide antigens.
28275152	1	62	theme	certain	345:351	arg1	serotypes					353:361	certain serotypes	345:361	certain serotypes	345:361	Glycoconjugate vaccines based on capsular polysaccharides (CPSs) of pathogenic bacteria such as Streptococcus pneumoniae successfully protect from disease but suffer from incomplete coverage, are troublesome to manufacture from isolated CPSs, and lack efficacy against certain serotypes.
28275152	8	63	theme	glycoconjugates	1488:1502	arg1	Coformulation					1467:1479	Coformulation	1467:1479	Coformulation of ST8 glycoconjugates with the marketed 13-valent glycoconjugate vaccine Prevnar 13	1467:1564	Coformulation of ST8 glycoconjugates with the marketed 13-valent glycoconjugate vaccine Prevnar 13 yielded a potent 14-valent S. pneumoniae vaccine.
28275152	6	64	theme	nonprotective	1303:1315	arg1	glycotopes					1317:1326	protective and nonprotective glycotopes	1288:1326	protective and nonprotective glycotopes	1288:1326	Immunization of mice with this semisynthetic glycoconjugate followed by generation and characterization of a protective mAb identified protective and nonprotective glycotopes.
28275152	6	65	theme	mice	1169:1172	arg1	Immunization					1153:1164	Immunization	1153:1164	Immunization of mice with this semisynthetic glycoconjugate followed by generation and characterization of a protective mAb	1153:1275	Immunization of mice with this semisynthetic glycoconjugate followed by generation and characterization of a protective mAb identified protective and nonprotective glycotopes.
28275152	8	66	theme	marketed	1513:1520	arg1	vaccine					1547:1553	the marketed 13-valent glycoconjugate vaccine Prevnar 13	1509:1564	the marketed 13-valent glycoconjugate vaccine Prevnar 13	1509:1564	Coformulation of ST8 glycoconjugates with the marketed 13-valent glycoconjugate vaccine Prevnar 13 yielded a potent 14-valent S. pneumoniae vaccine.
28275152	1	67	theme	incomplete	247:256	arg1	coverage					258:265	incomplete coverage	247:265	incomplete coverage	247:265	Glycoconjugate vaccines based on capsular polysaccharides (CPSs) of pathogenic bacteria such as Streptococcus pneumoniae successfully protect from disease but suffer from incomplete coverage, are troublesome to manufacture from isolated CPSs, and lack efficacy against certain serotypes.
28275152	4	68	theme	microarray	919:928	arg1	experiments					930:940	glycan microarray experiments	912:940	glycan microarray experiments	912:940	All four tetrasaccharide frameshifts of ST8 CPS were prepared by AGA and used in glycan microarray experiments to identify the glycotopes recognized by antibodies against ST8.
28275152	6	69	theme	mAb	1273:1275	arg1	characterization					1240:1255	characterization	1240:1255	characterization	1240:1255	Immunization of mice with this semisynthetic glycoconjugate followed by generation and characterization of a protective mAb identified protective and nonprotective glycotopes.
28275152	6	69	theme	mAb	1273:1275	arg1	generation					1225:1234	generation	1225:1234	generation	1225:1234	Immunization of mice with this semisynthetic glycoconjugate followed by generation and characterization of a protective mAb identified protective and nonprotective glycotopes.
28275152	1	70	theme	pathogenic	144:153	arg1	pneumoniae					186:195	Streptococcus pneumoniae	172:195	Streptococcus pneumoniae	172:195	Glycoconjugate vaccines based on capsular polysaccharides (CPSs) of pathogenic bacteria such as Streptococcus pneumoniae successfully protect from disease but suffer from incomplete coverage, are troublesome to manufacture from isolated CPSs, and lack efficacy against certain serotypes.
28275152	1	70	theme	pathogenic	144:153	arg1	bacteria					155:162	pathogenic bacteria	144:162	pathogenic bacteria such as Streptococcus pneumoniae	144:195	Glycoconjugate vaccines based on capsular polysaccharides (CPSs) of pathogenic bacteria such as Streptococcus pneumoniae successfully protect from disease but suffer from incomplete coverage, are troublesome to manufacture from isolated CPSs, and lack efficacy against certain serotypes.
28275152	3	71	theme	immunological	711:723	arg1	evaluation					725:734	immunological evaluation	711:734	immunological evaluation	711:734	We describe a medicinal chemistry strategy based on a combination of automated glycan assembly (AGA), glycan microarray-based monoclonal antibody (mAb) reverse engineering, and immunological evaluation in vivo to uncover a protective glycan epitope (glycotope) for S. pneumoniae serotype 8 (ST8).
28275152	3	72	theme	monoclonal	660:669	arg1	antibody					671:678	microarray-based monoclonal antibody	643:678	glycan microarray-based monoclonal antibody (mAb) reverse engineering	636:704	We describe a medicinal chemistry strategy based on a combination of automated glycan assembly (AGA), glycan microarray-based monoclonal antibody (mAb) reverse engineering, and immunological evaluation in vivo to uncover a protective glycan epitope (glycotope) for S. pneumoniae serotype 8 (ST8).
28275152	3	72	theme	monoclonal	660:669	arg1	mAb					681:683	mAb	681:683	mAb	681:683	We describe a medicinal chemistry strategy based on a combination of automated glycan assembly (AGA), glycan microarray-based monoclonal antibody (mAb) reverse engineering, and immunological evaluation in vivo to uncover a protective glycan epitope (glycotope) for S. pneumoniae serotype 8 (ST8).
28275152	1	73	theme	bacteria	155:162	arg1	CPSs					135:138	CPSs	135:138	CPSs	135:138	Glycoconjugate vaccines based on capsular polysaccharides (CPSs) of pathogenic bacteria such as Streptococcus pneumoniae successfully protect from disease but suffer from incomplete coverage, are troublesome to manufacture from isolated CPSs, and lack efficacy against certain serotypes.
28275152	1	73	theme	bacteria	155:162	arg1	polysaccharides					118:132	capsular polysaccharides	109:132	capsular polysaccharides (CPSs) of pathogenic bacteria such as Streptococcus pneumoniae	109:195	Glycoconjugate vaccines based on capsular polysaccharides (CPSs) of pathogenic bacteria such as Streptococcus pneumoniae successfully protect from disease but suffer from incomplete coverage, are troublesome to manufacture from isolated CPSs, and lack efficacy against certain serotypes.
28275152	6	74	theme	semisynthetic	1184:1196	arg1	glycoconjugate					1198:1211	this semisynthetic glycoconjugate	1179:1211	this semisynthetic glycoconjugate followed by generation and characterization of a protective mAb	1179:1275	Immunization of mice with this semisynthetic glycoconjugate followed by generation and characterization of a protective mAb identified protective and nonprotective glycotopes.
28275152	3	75	theme	antibody	671:678	arg1	engineering					694:704	glycan microarray-based monoclonal antibody (mAb) reverse engineering	636:704	glycan microarray-based monoclonal antibody (mAb) reverse engineering	636:704	We describe a medicinal chemistry strategy based on a combination of automated glycan assembly (AGA), glycan microarray-based monoclonal antibody (mAb) reverse engineering, and immunological evaluation in vivo to uncover a protective glycan epitope (glycotope) for S. pneumoniae serotype 8 (ST8).
28275152	7	76	theme	rabbits	1345:1351	arg1	Immunization					1329:1340	Immunization	1329:1340	Immunization of rabbits with semisynthetic ST8 glycoconjugates containing protective glycotopes	1329:1423	Immunization of rabbits with semisynthetic ST8 glycoconjugates containing protective glycotopes induced an antibacterial immune response.
26939940	7	0	from	changes	1676:1682	arg1	bioactivity					1687:1697	bioactivity	1687:1697	bioactivity profiling in unripened and ripened conditions in tomato	1687:1753	CONCLUSION This study, for the first time, showed changes in bioactivity profiling in unripened and ripened conditions in tomato.
26939940	5	1	theme	pectic	1341:1346	arg1	oligosaccharides					1348:1363	pectic oligosaccharides	1341:1363	pectic oligosaccharides (POs) than pectic polysaccharides (PPs)	1341:1403	The IC50 as determined by free radical scavenging (FRS), red blood cell (RBC) and DNA protection assays revealed reduction in FRS and RBC protective potencies in pectic oligosaccharides (POs) than pectic polysaccharides (PPs), supporting the fact that phenolics contribute towards these activities.
26939940	5	1	theme	pectic	1341:1346	arg1	POs					1366:1368	POs	1366:1368	POs	1366:1368	The IC50 as determined by free radical scavenging (FRS), red blood cell (RBC) and DNA protection assays revealed reduction in FRS and RBC protective potencies in pectic oligosaccharides (POs) than pectic polysaccharides (PPs), supporting the fact that phenolics contribute towards these activities.
26939940	2	2	theme	pectic	624:629	arg1	polysaccharides					631:645	pectic polysaccharides	624:645	pectic polysaccharides (PPs)	624:651	Antioxidant, cyto/DNA protection and galectin-3 inhibitory potentials were examined in pectic polysaccharides (PPs) and pectic oligosaccharides (POs) from four types of two varieties of tomatoes such as Sour (Mallika local variety) raw (SrRT-SrRTPP, SrRTPO), Sour ripe (SrRIT-SrRITPP, SrRITPO), Sweet (Rashmi local variety) raw (SwRT-SwRTPP, SwRTPO) and Sweet ripe (SwRIT-SwRITPP and SwRITPO).
26939940	2	2	theme	pectic	624:629	arg1	variety					760:766	Mallika local variety	746:766	Mallika local variety	746:766	Antioxidant, cyto/DNA protection and galectin-3 inhibitory potentials were examined in pectic polysaccharides (PPs) and pectic oligosaccharides (POs) from four types of two varieties of tomatoes such as Sour (Mallika local variety) raw (SrRT-SrRTPP, SrRTPO), Sour ripe (SrRIT-SrRITPP, SrRITPO), Sweet (Rashmi local variety) raw (SwRT-SwRTPP, SwRTPO) and Sweet ripe (SwRIT-SwRITPP and SwRITPO).
26939940	2	2	theme	pectic	624:629	arg1	Sour					740:743	Sour	740:743	Sour	740:743	Antioxidant, cyto/DNA protection and galectin-3 inhibitory potentials were examined in pectic polysaccharides (PPs) and pectic oligosaccharides (POs) from four types of two varieties of tomatoes such as Sour (Mallika local variety) raw (SrRT-SrRTPP, SrRTPO), Sour ripe (SrRIT-SrRITPP, SrRITPO), Sweet (Rashmi local variety) raw (SwRT-SwRTPP, SwRTPO) and Sweet ripe (SwRIT-SwRITPP and SwRITPO).
26939940	2	2	theme	pectic	624:629	arg1	PPs					648:650	PPs	648:650	PPs	648:650	Antioxidant, cyto/DNA protection and galectin-3 inhibitory potentials were examined in pectic polysaccharides (PPs) and pectic oligosaccharides (POs) from four types of two varieties of tomatoes such as Sour (Mallika local variety) raw (SrRT-SrRTPP, SrRTPO), Sour ripe (SrRIT-SrRITPP, SrRITPO), Sweet (Rashmi local variety) raw (SwRT-SwRTPP, SwRTPO) and Sweet ripe (SwRIT-SwRITPP and SwRITPO).
26939940	2	3	theme	Rashmi	839:844	arg1	raw					769:771	pectic polysaccharides (PPs) and pectic oligosaccharides (POs) from four types of two varieties of tomatoes such as Sour (Mallika local variety) raw	624:771	pectic polysaccharides (PPs) and pectic oligosaccharides (POs) from four types of two varieties of tomatoes such as Sour (Mallika local variety) raw (SrRT-SrRTPP, SrRTPO)	624:793	Antioxidant, cyto/DNA protection and galectin-3 inhibitory potentials were examined in pectic polysaccharides (PPs) and pectic oligosaccharides (POs) from four types of two varieties of tomatoes such as Sour (Mallika local variety) raw (SrRT-SrRTPP, SrRTPO), Sour ripe (SrRIT-SrRITPP, SrRITPO), Sweet (Rashmi local variety) raw (SwRT-SwRTPP, SwRTPO) and Sweet ripe (SwRIT-SwRITPP and SwRITPO).
26939940	2	3	theme	Rashmi	839:844	arg1	variety					852:858	Rashmi local variety	839:858	Rashmi local variety	839:858	Antioxidant, cyto/DNA protection and galectin-3 inhibitory potentials were examined in pectic polysaccharides (PPs) and pectic oligosaccharides (POs) from four types of two varieties of tomatoes such as Sour (Mallika local variety) raw (SrRT-SrRTPP, SrRTPO), Sour ripe (SrRIT-SrRITPP, SrRITPO), Sweet (Rashmi local variety) raw (SwRT-SwRTPP, SwRTPO) and Sweet ripe (SwRIT-SwRITPP and SwRITPO).
26939940	2	4	dep	raw	861:863	arg1	SwRITPO					921:927	SwRITPO	921:927	SwRITPO	921:927	Antioxidant, cyto/DNA protection and galectin-3 inhibitory potentials were examined in pectic polysaccharides (PPs) and pectic oligosaccharides (POs) from four types of two varieties of tomatoes such as Sour (Mallika local variety) raw (SrRT-SrRTPP, SrRTPO), Sour ripe (SrRIT-SrRITPP, SrRITPO), Sweet (Rashmi local variety) raw (SwRT-SwRTPP, SwRTPO) and Sweet ripe (SwRIT-SwRITPP and SwRITPO).
26939940	2	4	dep	raw	861:863	arg1	SwRIT-SwRITPP					903:915	SwRIT-SwRITPP	903:915	SwRIT-SwRITPP	903:915	Antioxidant, cyto/DNA protection and galectin-3 inhibitory potentials were examined in pectic polysaccharides (PPs) and pectic oligosaccharides (POs) from four types of two varieties of tomatoes such as Sour (Mallika local variety) raw (SrRT-SrRTPP, SrRTPO), Sour ripe (SrRIT-SrRITPP, SrRITPO), Sweet (Rashmi local variety) raw (SwRT-SwRTPP, SwRTPO) and Sweet ripe (SwRIT-SwRITPP and SwRITPO).
26939940	8	5	theme	Precise	1756:1762	arg1	alterations					1764:1774	Precise alterations	1756:1774	Precise alterations in biomolecular components, such as bound cinnamyl/ferulyl and vanillic acid derivatives	1756:1863	Precise alterations in biomolecular components, such as bound cinnamyl/ferulyl and vanillic acid derivatives, along with alterations in sugar composition that reflect changes in antioxidants, cyto/DNA protective and antimetastatic potentials, have been delineated.
26939940	2	6	theme	pectic	657:662	arg1	POs					682:684	POs	682:684	POs	682:684	Antioxidant, cyto/DNA protection and galectin-3 inhibitory potentials were examined in pectic polysaccharides (PPs) and pectic oligosaccharides (POs) from four types of two varieties of tomatoes such as Sour (Mallika local variety) raw (SrRT-SrRTPP, SrRTPO), Sour ripe (SrRIT-SrRITPP, SrRITPO), Sweet (Rashmi local variety) raw (SwRT-SwRTPP, SwRTPO) and Sweet ripe (SwRIT-SwRITPP and SwRITPO).
26939940	2	6	theme	pectic	657:662	arg1	oligosaccharides					664:679	pectic oligosaccharides	657:679	pectic oligosaccharides (POs) from four types of two varieties of tomatoes such as Sour	657:743	Antioxidant, cyto/DNA protection and galectin-3 inhibitory potentials were examined in pectic polysaccharides (PPs) and pectic oligosaccharides (POs) from four types of two varieties of tomatoes such as Sour (Mallika local variety) raw (SrRT-SrRTPP, SrRTPO), Sour ripe (SrRIT-SrRITPP, SrRITPO), Sweet (Rashmi local variety) raw (SwRT-SwRTPP, SwRTPO) and Sweet ripe (SwRIT-SwRITPP and SwRITPO).
26939940	2	6	theme	pectic	657:662	arg1	Sour					740:743	Sour	740:743	Sour	740:743	Antioxidant, cyto/DNA protection and galectin-3 inhibitory potentials were examined in pectic polysaccharides (PPs) and pectic oligosaccharides (POs) from four types of two varieties of tomatoes such as Sour (Mallika local variety) raw (SrRT-SrRTPP, SrRTPO), Sour ripe (SrRIT-SrRITPP, SrRITPO), Sweet (Rashmi local variety) raw (SwRT-SwRTPP, SwRTPO) and Sweet ripe (SwRIT-SwRITPP and SwRITPO).
26939940	4	7	dep	10-fold	1098:1104	arg1	to					1095:1096	to	1095:1096	to	1095:1096	An approximately nine- to 10-fold increase in galectin-3 inhibitory activity in sour variety was observed.
26939940	1	8	theme	protective	198:207	arg1	potentials					209:218	BACKGROUND Antioxidant, cyto/DNA protective potentials	165:218	potentials	209:218	BACKGROUND Antioxidant, cyto/DNA protective potentials are known to offer significant protection against free radical induced injury to cells or tissues and cellular damages that are envisaged in various diseases including chronic diseases like cancer, diabetes, etc, while galectin-3 inhibitory potentials are known to block or delay the process of metastasis in cancer.
26939940	8	9	dep	along	1866:1870	arg1	with					1872:1875	with	1872:1875	with	1872:1875	Precise alterations in biomolecular components, such as bound cinnamyl/ferulyl and vanillic acid derivatives, along with alterations in sugar composition that reflect changes in antioxidants, cyto/DNA protective and antimetastatic potentials, have been delineated.
26939940	2	10	theme	Mallika	746:752	arg1	variety					760:766	Mallika local variety	746:766	Mallika local variety	746:766	Antioxidant, cyto/DNA protection and galectin-3 inhibitory potentials were examined in pectic polysaccharides (PPs) and pectic oligosaccharides (POs) from four types of two varieties of tomatoes such as Sour (Mallika local variety) raw (SrRT-SrRTPP, SrRTPO), Sour ripe (SrRIT-SrRITPP, SrRITPO), Sweet (Rashmi local variety) raw (SwRT-SwRTPP, SwRTPO) and Sweet ripe (SwRIT-SwRITPP and SwRITPO).
26939940	2	10	theme	Mallika	746:752	arg1	polysaccharides					631:645	pectic polysaccharides	624:645	pectic polysaccharides (PPs)	624:651	Antioxidant, cyto/DNA protection and galectin-3 inhibitory potentials were examined in pectic polysaccharides (PPs) and pectic oligosaccharides (POs) from four types of two varieties of tomatoes such as Sour (Mallika local variety) raw (SrRT-SrRTPP, SrRTPO), Sour ripe (SrRIT-SrRITPP, SrRITPO), Sweet (Rashmi local variety) raw (SwRT-SwRTPP, SwRTPO) and Sweet ripe (SwRIT-SwRITPP and SwRITPO).
26939940	2	10	theme	Mallika	746:752	arg1	Sour					740:743	Sour	740:743	Sour	740:743	Antioxidant, cyto/DNA protection and galectin-3 inhibitory potentials were examined in pectic polysaccharides (PPs) and pectic oligosaccharides (POs) from four types of two varieties of tomatoes such as Sour (Mallika local variety) raw (SrRT-SrRTPP, SrRTPO), Sour ripe (SrRIT-SrRITPP, SrRITPO), Sweet (Rashmi local variety) raw (SwRT-SwRTPP, SwRTPO) and Sweet ripe (SwRIT-SwRITPP and SwRITPO).
26939940	2	11	theme	galectin-3	574:583	arg1	potentials					596:605	galectin-3 inhibitory potentials	574:605	galectin-3 inhibitory potentials	574:605	Antioxidant, cyto/DNA protection and galectin-3 inhibitory potentials were examined in pectic polysaccharides (PPs) and pectic oligosaccharides (POs) from four types of two varieties of tomatoes such as Sour (Mallika local variety) raw (SrRT-SrRTPP, SrRTPO), Sour ripe (SrRIT-SrRITPP, SrRITPO), Sweet (Rashmi local variety) raw (SwRT-SwRTPP, SwRTPO) and Sweet ripe (SwRIT-SwRITPP and SwRITPO).
26939940	0	12	from	potentials	88:97	arg1	poly/oligosaccharides					109:129	pectic poly/oligosaccharides	102:129	pectic poly/oligosaccharides of tomato (Solanum lycopersicum)	102:162	Physiologically induced changes in bound phenolics and antioxidants, DNA/cytoprotective potentials in pectic poly/oligosaccharides of tomato (Solanum lycopersicum).
26939940	8	13	theme	bound	1812:1816	arg1	cinnamyl/ferulyl					1818:1833	bound cinnamyl/ferulyl	1812:1833	bound cinnamyl/ferulyl	1812:1833	Precise alterations in biomolecular components, such as bound cinnamyl/ferulyl and vanillic acid derivatives, along with alterations in sugar composition that reflect changes in antioxidants, cyto/DNA protective and antimetastatic potentials, have been delineated.
26939940	1	14	theme	significant	239:249	arg1	protection					251:260	significant protection	239:260	significant protection against free radical induced injury to cells or tissues and cellular damages that are envisaged in various diseases including chronic diseases like cancer, diabetes, etc, while galectin-3 inhibitory potentials are known to block or delay the process of metastasis in cancer	239:534	BACKGROUND Antioxidant, cyto/DNA protective potentials are known to offer significant protection against free radical induced injury to cells or tissues and cellular damages that are envisaged in various diseases including chronic diseases like cancer, diabetes, etc, while galectin-3 inhibitory potentials are known to block or delay the process of metastasis in cancer.
26939940	4	15	theme	galectin-3	1118:1127	arg1	activity					1140:1147	galectin-3 inhibitory activity	1118:1147	galectin-3 inhibitory activity in sour variety	1118:1163	An approximately nine- to 10-fold increase in galectin-3 inhibitory activity in sour variety was observed.
26939940	8	16	theme	acid	1848:1851	arg1	derivatives					1853:1863	vanillic acid derivatives	1839:1863	vanillic acid derivatives	1839:1863	Precise alterations in biomolecular components, such as bound cinnamyl/ferulyl and vanillic acid derivatives, along with alterations in sugar composition that reflect changes in antioxidants, cyto/DNA protective and antimetastatic potentials, have been delineated.
26939940	1	17	theme	galectin-3	439:448	arg1	potentials					461:470	galectin-3 inhibitory potentials	439:470	galectin-3 inhibitory potentials	439:470	BACKGROUND Antioxidant, cyto/DNA protective potentials are known to offer significant protection against free radical induced injury to cells or tissues and cellular damages that are envisaged in various diseases including chronic diseases like cancer, diabetes, etc, while galectin-3 inhibitory potentials are known to block or delay the process of metastasis in cancer.
26939940	6	18	theme	ripening	1565:1572	arg1	process					1574:1580	the ripening process	1561:1580	the ripening process	1561:1580	Loss of activity could be attributed to the hydrolysis of certain phenolics during the ripening process as well as during conversion of PPs to POs.
26939940	1	19	theme	BACKGROUND	165:174	arg1	potentials					209:218	BACKGROUND Antioxidant, cyto/DNA protective potentials	165:218	potentials	209:218	BACKGROUND Antioxidant, cyto/DNA protective potentials are known to offer significant protection against free radical induced injury to cells or tissues and cellular damages that are envisaged in various diseases including chronic diseases like cancer, diabetes, etc, while galectin-3 inhibitory potentials are known to block or delay the process of metastasis in cancer.
26939940	0	20	from	changes	24:30	arg1	phenolics					41:49	bound phenolics	35:49	bound phenolics	35:49	Physiologically induced changes in bound phenolics and antioxidants, DNA/cytoprotective potentials in pectic poly/oligosaccharides of tomato (Solanum lycopersicum).
26939940	0	20	from	changes	24:30	arg1	antioxidants					55:66	antioxidants	55:66	antioxidants	55:66	Physiologically induced changes in bound phenolics and antioxidants, DNA/cytoprotective potentials in pectic poly/oligosaccharides of tomato (Solanum lycopersicum).
26939940	2	21	theme	Sweet	891:895	arg1	ripe					897:900	raw (SwRT-SwRTPP, SwRTPO) and Sweet ripe	861:900	ripe	897:900	Antioxidant, cyto/DNA protection and galectin-3 inhibitory potentials were examined in pectic polysaccharides (PPs) and pectic oligosaccharides (POs) from four types of two varieties of tomatoes such as Sour (Mallika local variety) raw (SrRT-SrRTPP, SrRTPO), Sour ripe (SrRIT-SrRITPP, SrRITPO), Sweet (Rashmi local variety) raw (SwRT-SwRTPP, SwRTPO) and Sweet ripe (SwRIT-SwRITPP and SwRITPO).
26939940	5	22	theme	radical	1210:1216	arg1	FRS					1230:1232	FRS	1230:1232	FRS	1230:1232	The IC50 as determined by free radical scavenging (FRS), red blood cell (RBC) and DNA protection assays revealed reduction in FRS and RBC protective potencies in pectic oligosaccharides (POs) than pectic polysaccharides (PPs), supporting the fact that phenolics contribute towards these activities.
26939940	5	22	theme	radical	1210:1216	arg1	scavenging					1218:1227	free radical scavenging	1205:1227	free radical scavenging (FRS)	1205:1233	The IC50 as determined by free radical scavenging (FRS), red blood cell (RBC) and DNA protection assays revealed reduction in FRS and RBC protective potencies in pectic oligosaccharides (POs) than pectic polysaccharides (PPs), supporting the fact that phenolics contribute towards these activities.
26939940	0	23	theme	pectic	102:107	arg1	poly/oligosaccharides					109:129	pectic poly/oligosaccharides	102:129	pectic poly/oligosaccharides of tomato (Solanum lycopersicum)	102:162	Physiologically induced changes in bound phenolics and antioxidants, DNA/cytoprotective potentials in pectic poly/oligosaccharides of tomato (Solanum lycopersicum).
26939940	1	24	theme	chronic	388:394	arg1	diseases					396:403	chronic diseases	388:403	chronic diseases like cancer, diabetes, etc	388:430	BACKGROUND Antioxidant, cyto/DNA protective potentials are known to offer significant protection against free radical induced injury to cells or tissues and cellular damages that are envisaged in various diseases including chronic diseases like cancer, diabetes, etc, while galectin-3 inhibitory potentials are known to block or delay the process of metastasis in cancer.
26939940	6	25	theme	phenolics	1544:1552	arg1	hydrolysis					1522:1531	the hydrolysis	1518:1531	the hydrolysis of certain phenolics during the ripening process as well as during conversion of PPs to POs	1518:1623	Loss of activity could be attributed to the hydrolysis of certain phenolics during the ripening process as well as during conversion of PPs to POs.
26939940	5	26	dep	FRS	1305:1307	arg1	potencies					1328:1336	protective potencies	1317:1336	protective potencies	1317:1336	The IC50 as determined by free radical scavenging (FRS), red blood cell (RBC) and DNA protection assays revealed reduction in FRS and RBC protective potencies in pectic oligosaccharides (POs) than pectic polysaccharides (PPs), supporting the fact that phenolics contribute towards these activities.
26939940	2	27	theme	oligosaccharides	664:679	arg1	SrRTPO					787:792	SrRTPO	787:792	SrRTPO	787:792	Antioxidant, cyto/DNA protection and galectin-3 inhibitory potentials were examined in pectic polysaccharides (PPs) and pectic oligosaccharides (POs) from four types of two varieties of tomatoes such as Sour (Mallika local variety) raw (SrRT-SrRTPP, SrRTPO), Sour ripe (SrRIT-SrRITPP, SrRITPO), Sweet (Rashmi local variety) raw (SwRT-SwRTPP, SwRTPO) and Sweet ripe (SwRIT-SwRITPP and SwRITPO).
26939940	2	27	theme	oligosaccharides	664:679	arg1	Sweet					832:836	Sweet	832:836	Sweet	832:836	Antioxidant, cyto/DNA protection and galectin-3 inhibitory potentials were examined in pectic polysaccharides (PPs) and pectic oligosaccharides (POs) from four types of two varieties of tomatoes such as Sour (Mallika local variety) raw (SrRT-SrRTPP, SrRTPO), Sour ripe (SrRIT-SrRITPP, SrRITPO), Sweet (Rashmi local variety) raw (SwRT-SwRTPP, SwRTPO) and Sweet ripe (SwRIT-SwRITPP and SwRITPO).
26939940	2	27	theme	oligosaccharides	664:679	arg1	ripe					801:804	Sour ripe	796:804	Sour ripe (SrRIT-SrRITPP, SrRITPO)	796:829	Antioxidant, cyto/DNA protection and galectin-3 inhibitory potentials were examined in pectic polysaccharides (PPs) and pectic oligosaccharides (POs) from four types of two varieties of tomatoes such as Sour (Mallika local variety) raw (SrRT-SrRTPP, SrRTPO), Sour ripe (SrRIT-SrRITPP, SrRITPO), Sweet (Rashmi local variety) raw (SwRT-SwRTPP, SwRTPO) and Sweet ripe (SwRIT-SwRITPP and SwRITPO).
26939940	2	27	theme	oligosaccharides	664:679	arg1	raw					769:771	pectic polysaccharides (PPs) and pectic oligosaccharides (POs) from four types of two varieties of tomatoes such as Sour (Mallika local variety) raw	624:771	pectic polysaccharides (PPs) and pectic oligosaccharides (POs) from four types of two varieties of tomatoes such as Sour (Mallika local variety) raw (SrRT-SrRTPP, SrRTPO)	624:793	Antioxidant, cyto/DNA protection and galectin-3 inhibitory potentials were examined in pectic polysaccharides (PPs) and pectic oligosaccharides (POs) from four types of two varieties of tomatoes such as Sour (Mallika local variety) raw (SrRT-SrRTPP, SrRTPO), Sour ripe (SrRIT-SrRITPP, SrRITPO), Sweet (Rashmi local variety) raw (SwRT-SwRTPP, SwRTPO) and Sweet ripe (SwRIT-SwRITPP and SwRITPO).
26939940	2	27	theme	oligosaccharides	664:679	arg1	variety					852:858	Rashmi local variety	839:858	Rashmi local variety	839:858	Antioxidant, cyto/DNA protection and galectin-3 inhibitory potentials were examined in pectic polysaccharides (PPs) and pectic oligosaccharides (POs) from four types of two varieties of tomatoes such as Sour (Mallika local variety) raw (SrRT-SrRTPP, SrRTPO), Sour ripe (SrRIT-SrRITPP, SrRITPO), Sweet (Rashmi local variety) raw (SwRT-SwRTPP, SwRTPO) and Sweet ripe (SwRIT-SwRITPP and SwRITPO).
26939940	3	28	theme	inhibitory	1036:1045	arg1	property					1047:1054	approximately four- to five-fold better galectin-3 inhibitory property	985:1054	approximately four- to five-fold better galectin-3 inhibitory property	985:1054	RESULTS Results indicate that unripe PPs and POs show approximately four- to five-fold better galectin-3 inhibitory property than ripe ones.
26939940	8	29	theme	antimetastatic	1972:1985	arg1	potentials					1987:1996	cyto/DNA protective and antimetastatic potentials	1948:1996	cyto/DNA protective and antimetastatic potentials	1948:1996	Precise alterations in biomolecular components, such as bound cinnamyl/ferulyl and vanillic acid derivatives, along with alterations in sugar composition that reflect changes in antioxidants, cyto/DNA protective and antimetastatic potentials, have been delineated.
26939940	6	30	theme	PPs	1614:1616	arg1	conversion					1600:1609	conversion	1600:1609	conversion of PPs to POs	1600:1623	Loss of activity could be attributed to the hydrolysis of certain phenolics during the ripening process as well as during conversion of PPs to POs.
26939940	5	31	theme	pectic	1376:1381	arg1	PPs					1400:1402	PPs	1400:1402	PPs	1400:1402	The IC50 as determined by free radical scavenging (FRS), red blood cell (RBC) and DNA protection assays revealed reduction in FRS and RBC protective potencies in pectic oligosaccharides (POs) than pectic polysaccharides (PPs), supporting the fact that phenolics contribute towards these activities.
26939940	5	31	theme	pectic	1376:1381	arg1	polysaccharides					1383:1397	pectic polysaccharides	1376:1397	pectic polysaccharides (PPs)	1376:1403	The IC50 as determined by free radical scavenging (FRS), red blood cell (RBC) and DNA protection assays revealed reduction in FRS and RBC protective potencies in pectic oligosaccharides (POs) than pectic polysaccharides (PPs), supporting the fact that phenolics contribute towards these activities.
26939940	4	32	theme	sour	1152:1155	arg1	variety					1157:1163	sour variety	1152:1163	sour variety	1152:1163	An approximately nine- to 10-fold increase in galectin-3 inhibitory activity in sour variety was observed.
26939940	5	33	theme	red	1236:1238	arg1	RBC					1252:1254	RBC	1252:1254	RBC	1252:1254	The IC50 as determined by free radical scavenging (FRS), red blood cell (RBC) and DNA protection assays revealed reduction in FRS and RBC protective potencies in pectic oligosaccharides (POs) than pectic polysaccharides (PPs), supporting the fact that phenolics contribute towards these activities.
26939940	5	33	theme	red	1236:1238	arg1	cell					1246:1249	red blood cell	1236:1249	red blood cell (RBC)	1236:1255	The IC50 as determined by free radical scavenging (FRS), red blood cell (RBC) and DNA protection assays revealed reduction in FRS and RBC protective potencies in pectic oligosaccharides (POs) than pectic polysaccharides (PPs), supporting the fact that phenolics contribute towards these activities.
26939940	1	34	from	process	504:510	arg1	cancer					529:534	cancer	529:534	cancer	529:534	BACKGROUND Antioxidant, cyto/DNA protective potentials are known to offer significant protection against free radical induced injury to cells or tissues and cellular damages that are envisaged in various diseases including chronic diseases like cancer, diabetes, etc, while galectin-3 inhibitory potentials are known to block or delay the process of metastasis in cancer.
26939940	0	35	theme	bound	35:39	arg1	phenolics					41:49	bound phenolics	35:49	bound phenolics	35:49	Physiologically induced changes in bound phenolics and antioxidants, DNA/cytoprotective potentials in pectic poly/oligosaccharides of tomato (Solanum lycopersicum).
26939940	4	36	from	activity	1140:1147	arg1	variety					1157:1163	sour variety	1152:1163	sour variety	1152:1163	An approximately nine- to 10-fold increase in galectin-3 inhibitory activity in sour variety was observed.
26939940	8	37	theme	protective	1957:1966	arg1	potentials					1987:1996	cyto/DNA protective and antimetastatic potentials	1948:1996	cyto/DNA protective and antimetastatic potentials	1948:1996	Precise alterations in biomolecular components, such as bound cinnamyl/ferulyl and vanillic acid derivatives, along with alterations in sugar composition that reflect changes in antioxidants, cyto/DNA protective and antimetastatic potentials, have been delineated.
26939940	2	38	theme	Sour	796:799	arg1	SrRITPO					822:828	SrRITPO	822:828	SrRITPO	822:828	Antioxidant, cyto/DNA protection and galectin-3 inhibitory potentials were examined in pectic polysaccharides (PPs) and pectic oligosaccharides (POs) from four types of two varieties of tomatoes such as Sour (Mallika local variety) raw (SrRT-SrRTPP, SrRTPO), Sour ripe (SrRIT-SrRITPP, SrRITPO), Sweet (Rashmi local variety) raw (SwRT-SwRTPP, SwRTPO) and Sweet ripe (SwRIT-SwRITPP and SwRITPO).
26939940	2	38	theme	Sour	796:799	arg1	raw					769:771	pectic polysaccharides (PPs) and pectic oligosaccharides (POs) from four types of two varieties of tomatoes such as Sour (Mallika local variety) raw	624:771	pectic polysaccharides (PPs) and pectic oligosaccharides (POs) from four types of two varieties of tomatoes such as Sour (Mallika local variety) raw (SrRT-SrRTPP, SrRTPO)	624:793	Antioxidant, cyto/DNA protection and galectin-3 inhibitory potentials were examined in pectic polysaccharides (PPs) and pectic oligosaccharides (POs) from four types of two varieties of tomatoes such as Sour (Mallika local variety) raw (SrRT-SrRTPP, SrRTPO), Sour ripe (SrRIT-SrRITPP, SrRITPO), Sweet (Rashmi local variety) raw (SwRT-SwRTPP, SwRTPO) and Sweet ripe (SwRIT-SwRITPP and SwRITPO).
26939940	2	38	theme	Sour	796:799	arg1	ripe					801:804	Sour ripe	796:804	Sour ripe (SrRIT-SrRITPP, SrRITPO)	796:829	Antioxidant, cyto/DNA protection and galectin-3 inhibitory potentials were examined in pectic polysaccharides (PPs) and pectic oligosaccharides (POs) from four types of two varieties of tomatoes such as Sour (Mallika local variety) raw (SrRT-SrRTPP, SrRTPO), Sour ripe (SrRIT-SrRITPP, SrRITPO), Sweet (Rashmi local variety) raw (SwRT-SwRTPP, SwRTPO) and Sweet ripe (SwRIT-SwRITPP and SwRITPO).
26939940	4	39	from	increase	1106:1113	arg1	activity					1140:1147	galectin-3 inhibitory activity	1118:1147	galectin-3 inhibitory activity in sour variety	1118:1163	An approximately nine- to 10-fold increase in galectin-3 inhibitory activity in sour variety was observed.
26939940	5	40	theme	protection	1265:1274	arg1	assays					1276:1281	DNA protection assays	1261:1281	DNA protection assays	1261:1281	The IC50 as determined by free radical scavenging (FRS), red blood cell (RBC) and DNA protection assays revealed reduction in FRS and RBC protective potencies in pectic oligosaccharides (POs) than pectic polysaccharides (PPs), supporting the fact that phenolics contribute towards these activities.
26939940	1	41	theme	free	270:273	arg1	radical					275:281	free radical	270:281	free radical induced injury to cells or tissues and cellular damages that are envisaged in various diseases including chronic diseases like cancer, diabetes, etc, while galectin-3 inhibitory potentials are known to block or delay the process of metastasis in cancer	270:534	BACKGROUND Antioxidant, cyto/DNA protective potentials are known to offer significant protection against free radical induced injury to cells or tissues and cellular damages that are envisaged in various diseases including chronic diseases like cancer, diabetes, etc, while galectin-3 inhibitory potentials are known to block or delay the process of metastasis in cancer.
26939940	2	42	theme	polysaccharides	631:645	arg1	SrRTPO					787:792	SrRTPO	787:792	SrRTPO	787:792	Antioxidant, cyto/DNA protection and galectin-3 inhibitory potentials were examined in pectic polysaccharides (PPs) and pectic oligosaccharides (POs) from four types of two varieties of tomatoes such as Sour (Mallika local variety) raw (SrRT-SrRTPP, SrRTPO), Sour ripe (SrRIT-SrRITPP, SrRITPO), Sweet (Rashmi local variety) raw (SwRT-SwRTPP, SwRTPO) and Sweet ripe (SwRIT-SwRITPP and SwRITPO).
26939940	2	42	theme	polysaccharides	631:645	arg1	Sweet					832:836	Sweet	832:836	Sweet	832:836	Antioxidant, cyto/DNA protection and galectin-3 inhibitory potentials were examined in pectic polysaccharides (PPs) and pectic oligosaccharides (POs) from four types of two varieties of tomatoes such as Sour (Mallika local variety) raw (SrRT-SrRTPP, SrRTPO), Sour ripe (SrRIT-SrRITPP, SrRITPO), Sweet (Rashmi local variety) raw (SwRT-SwRTPP, SwRTPO) and Sweet ripe (SwRIT-SwRITPP and SwRITPO).
26939940	2	42	theme	polysaccharides	631:645	arg1	ripe					801:804	Sour ripe	796:804	Sour ripe (SrRIT-SrRITPP, SrRITPO)	796:829	Antioxidant, cyto/DNA protection and galectin-3 inhibitory potentials were examined in pectic polysaccharides (PPs) and pectic oligosaccharides (POs) from four types of two varieties of tomatoes such as Sour (Mallika local variety) raw (SrRT-SrRTPP, SrRTPO), Sour ripe (SrRIT-SrRITPP, SrRITPO), Sweet (Rashmi local variety) raw (SwRT-SwRTPP, SwRTPO) and Sweet ripe (SwRIT-SwRITPP and SwRITPO).
26939940	2	42	theme	polysaccharides	631:645	arg1	raw					769:771	pectic polysaccharides (PPs) and pectic oligosaccharides (POs) from four types of two varieties of tomatoes such as Sour (Mallika local variety) raw	624:771	pectic polysaccharides (PPs) and pectic oligosaccharides (POs) from four types of two varieties of tomatoes such as Sour (Mallika local variety) raw (SrRT-SrRTPP, SrRTPO)	624:793	Antioxidant, cyto/DNA protection and galectin-3 inhibitory potentials were examined in pectic polysaccharides (PPs) and pectic oligosaccharides (POs) from four types of two varieties of tomatoes such as Sour (Mallika local variety) raw (SrRT-SrRTPP, SrRTPO), Sour ripe (SrRIT-SrRITPP, SrRITPO), Sweet (Rashmi local variety) raw (SwRT-SwRTPP, SwRTPO) and Sweet ripe (SwRIT-SwRITPP and SwRITPO).
26939940	2	42	theme	polysaccharides	631:645	arg1	variety					852:858	Rashmi local variety	839:858	Rashmi local variety	839:858	Antioxidant, cyto/DNA protection and galectin-3 inhibitory potentials were examined in pectic polysaccharides (PPs) and pectic oligosaccharides (POs) from four types of two varieties of tomatoes such as Sour (Mallika local variety) raw (SrRT-SrRTPP, SrRTPO), Sour ripe (SrRIT-SrRITPP, SrRITPO), Sweet (Rashmi local variety) raw (SwRT-SwRTPP, SwRTPO) and Sweet ripe (SwRIT-SwRITPP and SwRITPO).
26939940	2	43	theme	local	846:850	arg1	raw					769:771	pectic polysaccharides (PPs) and pectic oligosaccharides (POs) from four types of two varieties of tomatoes such as Sour (Mallika local variety) raw	624:771	pectic polysaccharides (PPs) and pectic oligosaccharides (POs) from four types of two varieties of tomatoes such as Sour (Mallika local variety) raw (SrRT-SrRTPP, SrRTPO)	624:793	Antioxidant, cyto/DNA protection and galectin-3 inhibitory potentials were examined in pectic polysaccharides (PPs) and pectic oligosaccharides (POs) from four types of two varieties of tomatoes such as Sour (Mallika local variety) raw (SrRT-SrRTPP, SrRTPO), Sour ripe (SrRIT-SrRITPP, SrRITPO), Sweet (Rashmi local variety) raw (SwRT-SwRTPP, SwRTPO) and Sweet ripe (SwRIT-SwRITPP and SwRITPO).
26939940	2	43	theme	local	846:850	arg1	variety					852:858	Rashmi local variety	839:858	Rashmi local variety	839:858	Antioxidant, cyto/DNA protection and galectin-3 inhibitory potentials were examined in pectic polysaccharides (PPs) and pectic oligosaccharides (POs) from four types of two varieties of tomatoes such as Sour (Mallika local variety) raw (SrRT-SrRTPP, SrRTPO), Sour ripe (SrRIT-SrRITPP, SrRITPO), Sweet (Rashmi local variety) raw (SwRT-SwRTPP, SwRTPO) and Sweet ripe (SwRIT-SwRITPP and SwRITPO).
26939940	9	44	theme	Chemical	2039:2046	arg1	Industry					2048:2055	Chemical Industry	2039:2055	Chemical Industry	2039:2055	© 2016 Society of Chemical Industry.
26939940	3	45	theme	RESULTS	931:937	arg1	Results					939:945	RESULTS Results	931:945	RESULTS Results	931:945	RESULTS Results indicate that unripe PPs and POs show approximately four- to five-fold better galectin-3 inhibitory property than ripe ones.
26939940	8	46	theme	biomolecular	1779:1790	arg1	cinnamyl/ferulyl					1818:1833	bound cinnamyl/ferulyl	1812:1833	bound cinnamyl/ferulyl	1812:1833	Precise alterations in biomolecular components, such as bound cinnamyl/ferulyl and vanillic acid derivatives, along with alterations in sugar composition that reflect changes in antioxidants, cyto/DNA protective and antimetastatic potentials, have been delineated.
26939940	8	46	theme	biomolecular	1779:1790	arg1	components					1792:1801	biomolecular components	1779:1801	biomolecular components	1779:1801	Precise alterations in biomolecular components, such as bound cinnamyl/ferulyl and vanillic acid derivatives, along with alterations in sugar composition that reflect changes in antioxidants, cyto/DNA protective and antimetastatic potentials, have been delineated.
26939940	8	46	theme	biomolecular	1779:1790	arg1	derivatives					1853:1863	vanillic acid derivatives	1839:1863	vanillic acid derivatives	1839:1863	Precise alterations in biomolecular components, such as bound cinnamyl/ferulyl and vanillic acid derivatives, along with alterations in sugar composition that reflect changes in antioxidants, cyto/DNA protective and antimetastatic potentials, have been delineated.
26939940	1	47	theme	cyto/DNA	189:196	arg1	potentials					209:218	BACKGROUND Antioxidant, cyto/DNA protective potentials	165:218	potentials	209:218	BACKGROUND Antioxidant, cyto/DNA protective potentials are known to offer significant protection against free radical induced injury to cells or tissues and cellular damages that are envisaged in various diseases including chronic diseases like cancer, diabetes, etc, while galectin-3 inhibitory potentials are known to block or delay the process of metastasis in cancer.
26939940	7	48	theme	first	1657:1661	arg1	time					1663:1666	the first time	1653:1666	the first time	1653:1666	CONCLUSION This study, for the first time, showed changes in bioactivity profiling in unripened and ripened conditions in tomato.
26939940	2	49	theme	local	754:758	arg1	variety					760:766	Mallika local variety	746:766	Mallika local variety	746:766	Antioxidant, cyto/DNA protection and galectin-3 inhibitory potentials were examined in pectic polysaccharides (PPs) and pectic oligosaccharides (POs) from four types of two varieties of tomatoes such as Sour (Mallika local variety) raw (SrRT-SrRTPP, SrRTPO), Sour ripe (SrRIT-SrRITPP, SrRITPO), Sweet (Rashmi local variety) raw (SwRT-SwRTPP, SwRTPO) and Sweet ripe (SwRIT-SwRITPP and SwRITPO).
26939940	2	49	theme	local	754:758	arg1	polysaccharides					631:645	pectic polysaccharides	624:645	pectic polysaccharides (PPs)	624:651	Antioxidant, cyto/DNA protection and galectin-3 inhibitory potentials were examined in pectic polysaccharides (PPs) and pectic oligosaccharides (POs) from four types of two varieties of tomatoes such as Sour (Mallika local variety) raw (SrRT-SrRTPP, SrRTPO), Sour ripe (SrRIT-SrRITPP, SrRITPO), Sweet (Rashmi local variety) raw (SwRT-SwRTPP, SwRTPO) and Sweet ripe (SwRIT-SwRITPP and SwRITPO).
26939940	2	49	theme	local	754:758	arg1	Sour					740:743	Sour	740:743	Sour	740:743	Antioxidant, cyto/DNA protection and galectin-3 inhibitory potentials were examined in pectic polysaccharides (PPs) and pectic oligosaccharides (POs) from four types of two varieties of tomatoes such as Sour (Mallika local variety) raw (SrRT-SrRTPP, SrRTPO), Sour ripe (SrRIT-SrRITPP, SrRITPO), Sweet (Rashmi local variety) raw (SwRT-SwRTPP, SwRTPO) and Sweet ripe (SwRIT-SwRITPP and SwRITPO).
26939940	5	50	theme	protective	1317:1326	arg1	potencies					1328:1336	protective potencies	1317:1336	protective potencies	1317:1336	The IC50 as determined by free radical scavenging (FRS), red blood cell (RBC) and DNA protection assays revealed reduction in FRS and RBC protective potencies in pectic oligosaccharides (POs) than pectic polysaccharides (PPs), supporting the fact that phenolics contribute towards these activities.
26939940	3	51	theme	unripe	961:966	arg1	PPs					968:970	unripe PPs	961:970	unripe PPs	961:970	RESULTS Results indicate that unripe PPs and POs show approximately four- to five-fold better galectin-3 inhibitory property than ripe ones.
26939940	8	52	from	alterations	1877:1887	arg1	composition					1898:1908	sugar composition	1892:1908	sugar composition that reflect changes in antioxidants	1892:1945	Precise alterations in biomolecular components, such as bound cinnamyl/ferulyl and vanillic acid derivatives, along with alterations in sugar composition that reflect changes in antioxidants, cyto/DNA protective and antimetastatic potentials, have been delineated.
26939940	2	53	theme	cyto/DNA	550:557	arg1	protection					559:568	cyto/DNA protection	550:568	cyto/DNA protection	550:568	Antioxidant, cyto/DNA protection and galectin-3 inhibitory potentials were examined in pectic polysaccharides (PPs) and pectic oligosaccharides (POs) from four types of two varieties of tomatoes such as Sour (Mallika local variety) raw (SrRT-SrRTPP, SrRTPO), Sour ripe (SrRIT-SrRITPP, SrRITPO), Sweet (Rashmi local variety) raw (SwRT-SwRTPP, SwRTPO) and Sweet ripe (SwRIT-SwRITPP and SwRITPO).
26939940	2	54	theme	tomatoes	723:730	arg1	types					697:701	four types	692:701	four types of two varieties of tomatoes	692:730	Antioxidant, cyto/DNA protection and galectin-3 inhibitory potentials were examined in pectic polysaccharides (PPs) and pectic oligosaccharides (POs) from four types of two varieties of tomatoes such as Sour (Mallika local variety) raw (SrRT-SrRTPP, SrRTPO), Sour ripe (SrRIT-SrRITPP, SrRITPO), Sweet (Rashmi local variety) raw (SwRT-SwRTPP, SwRTPO) and Sweet ripe (SwRIT-SwRITPP and SwRITPO).
26939940	8	55	theme	vanillic	1839:1846	arg1	derivatives					1853:1863	vanillic acid derivatives	1839:1863	vanillic acid derivatives	1839:1863	Precise alterations in biomolecular components, such as bound cinnamyl/ferulyl and vanillic acid derivatives, along with alterations in sugar composition that reflect changes in antioxidants, cyto/DNA protective and antimetastatic potentials, have been delineated.
26939940	2	56	theme	varieties	710:718	arg1	types					697:701	four types	692:701	four types of two varieties of tomatoes	692:730	Antioxidant, cyto/DNA protection and galectin-3 inhibitory potentials were examined in pectic polysaccharides (PPs) and pectic oligosaccharides (POs) from four types of two varieties of tomatoes such as Sour (Mallika local variety) raw (SrRT-SrRTPP, SrRTPO), Sour ripe (SrRIT-SrRITPP, SrRITPO), Sweet (Rashmi local variety) raw (SwRT-SwRTPP, SwRTPO) and Sweet ripe (SwRIT-SwRITPP and SwRITPO).
26939940	6	57	theme	activity	1486:1493	arg1	Loss					1478:1481	Loss	1478:1481	Loss of activity	1478:1493	Loss of activity could be attributed to the hydrolysis of certain phenolics during the ripening process as well as during conversion of PPs to POs.
26939940	2	58	theme	inhibitory	585:594	arg1	potentials					596:605	galectin-3 inhibitory potentials	574:605	galectin-3 inhibitory potentials	574:605	Antioxidant, cyto/DNA protection and galectin-3 inhibitory potentials were examined in pectic polysaccharides (PPs) and pectic oligosaccharides (POs) from four types of two varieties of tomatoes such as Sour (Mallika local variety) raw (SrRT-SrRTPP, SrRTPO), Sour ripe (SrRIT-SrRITPP, SrRITPO), Sweet (Rashmi local variety) raw (SwRT-SwRTPP, SwRTPO) and Sweet ripe (SwRIT-SwRITPP and SwRITPO).
26939940	4	59	theme	10-fold	1098:1104	arg1	increase					1106:1113	An approximately nine- to 10-fold increase	1072:1113	An approximately nine- to 10-fold increase in galectin-3 inhibitory activity in sour variety	1072:1163	An approximately nine- to 10-fold increase in galectin-3 inhibitory activity in sour variety was observed.
26939940	1	60	theme	inhibitory	450:459	arg1	potentials					461:470	galectin-3 inhibitory potentials	439:470	galectin-3 inhibitory potentials	439:470	BACKGROUND Antioxidant, cyto/DNA protective potentials are known to offer significant protection against free radical induced injury to cells or tissues and cellular damages that are envisaged in various diseases including chronic diseases like cancer, diabetes, etc, while galectin-3 inhibitory potentials are known to block or delay the process of metastasis in cancer.
26939940	7	61	from	conditions	1734:1743	arg1	tomato					1748:1753	tomato	1748:1753	tomato	1748:1753	CONCLUSION This study, for the first time, showed changes in bioactivity profiling in unripened and ripened conditions in tomato.
26939940	1	62	theme	various	361:367	arg1	diseases					369:376	various diseases	361:376	various diseases including chronic diseases like cancer, diabetes, etc	361:430	BACKGROUND Antioxidant, cyto/DNA protective potentials are known to offer significant protection against free radical induced injury to cells or tissues and cellular damages that are envisaged in various diseases including chronic diseases like cancer, diabetes, etc, while galectin-3 inhibitory potentials are known to block or delay the process of metastasis in cancer.
26939940	1	62	theme	various	361:367	arg1	diseases					396:403	chronic diseases	388:403	chronic diseases like cancer, diabetes, etc	388:430	BACKGROUND Antioxidant, cyto/DNA protective potentials are known to offer significant protection against free radical induced injury to cells or tissues and cellular damages that are envisaged in various diseases including chronic diseases like cancer, diabetes, etc, while galectin-3 inhibitory potentials are known to block or delay the process of metastasis in cancer.
26939940	1	63	theme	Antioxidant	176:186	arg1	potentials					209:218	BACKGROUND Antioxidant, cyto/DNA protective potentials	165:218	potentials	209:218	BACKGROUND Antioxidant, cyto/DNA protective potentials are known to offer significant protection against free radical induced injury to cells or tissues and cellular damages that are envisaged in various diseases including chronic diseases like cancer, diabetes, etc, while galectin-3 inhibitory potentials are known to block or delay the process of metastasis in cancer.
26939940	8	64	from	changes	1923:1929	arg1	antioxidants					1934:1945	antioxidants	1934:1945	antioxidants	1934:1945	Precise alterations in biomolecular components, such as bound cinnamyl/ferulyl and vanillic acid derivatives, along with alterations in sugar composition that reflect changes in antioxidants, cyto/DNA protective and antimetastatic potentials, have been delineated.
26939940	7	65	dep	CONCLUSION	1626:1635	arg1	showed					1669:1674	showed	1669:1674	showed changes in bioactivity profiling in unripened and ripened conditions in tomato	1669:1753	CONCLUSION This study, for the first time, showed changes in bioactivity profiling in unripened and ripened conditions in tomato.
26939940	2	66	from	types	697:701	arg1	polysaccharides					631:645	pectic polysaccharides	624:645	pectic polysaccharides (PPs)	624:651	Antioxidant, cyto/DNA protection and galectin-3 inhibitory potentials were examined in pectic polysaccharides (PPs) and pectic oligosaccharides (POs) from four types of two varieties of tomatoes such as Sour (Mallika local variety) raw (SrRT-SrRTPP, SrRTPO), Sour ripe (SrRIT-SrRITPP, SrRITPO), Sweet (Rashmi local variety) raw (SwRT-SwRTPP, SwRTPO) and Sweet ripe (SwRIT-SwRITPP and SwRITPO).
26939940	2	66	from	types	697:701	arg1	oligosaccharides					664:679	pectic oligosaccharides	657:679	pectic oligosaccharides (POs) from four types of two varieties of tomatoes such as Sour	657:743	Antioxidant, cyto/DNA protection and galectin-3 inhibitory potentials were examined in pectic polysaccharides (PPs) and pectic oligosaccharides (POs) from four types of two varieties of tomatoes such as Sour (Mallika local variety) raw (SrRT-SrRTPP, SrRTPO), Sour ripe (SrRIT-SrRITPP, SrRITPO), Sweet (Rashmi local variety) raw (SwRT-SwRTPP, SwRTPO) and Sweet ripe (SwRIT-SwRITPP and SwRITPO).
26939940	2	66	from	types	697:701	arg1	Sour					740:743	Sour	740:743	Sour	740:743	Antioxidant, cyto/DNA protection and galectin-3 inhibitory potentials were examined in pectic polysaccharides (PPs) and pectic oligosaccharides (POs) from four types of two varieties of tomatoes such as Sour (Mallika local variety) raw (SrRT-SrRTPP, SrRTPO), Sour ripe (SrRIT-SrRITPP, SrRITPO), Sweet (Rashmi local variety) raw (SwRT-SwRTPP, SwRTPO) and Sweet ripe (SwRIT-SwRITPP and SwRITPO).
26939940	2	66	from	types	697:701	arg1	POs					682:684	POs	682:684	POs	682:684	Antioxidant, cyto/DNA protection and galectin-3 inhibitory potentials were examined in pectic polysaccharides (PPs) and pectic oligosaccharides (POs) from four types of two varieties of tomatoes such as Sour (Mallika local variety) raw (SrRT-SrRTPP, SrRTPO), Sour ripe (SrRIT-SrRITPP, SrRITPO), Sweet (Rashmi local variety) raw (SwRT-SwRTPP, SwRTPO) and Sweet ripe (SwRIT-SwRITPP and SwRITPO).
26939940	2	66	from	types	697:701	arg1	PPs					648:650	PPs	648:650	PPs	648:650	Antioxidant, cyto/DNA protection and galectin-3 inhibitory potentials were examined in pectic polysaccharides (PPs) and pectic oligosaccharides (POs) from four types of two varieties of tomatoes such as Sour (Mallika local variety) raw (SrRT-SrRTPP, SrRTPO), Sour ripe (SrRIT-SrRITPP, SrRITPO), Sweet (Rashmi local variety) raw (SwRT-SwRTPP, SwRTPO) and Sweet ripe (SwRIT-SwRITPP and SwRITPO).
26939940	2	66	from	types	697:701	arg1	variety					760:766	Mallika local variety	746:766	Mallika local variety	746:766	Antioxidant, cyto/DNA protection and galectin-3 inhibitory potentials were examined in pectic polysaccharides (PPs) and pectic oligosaccharides (POs) from four types of two varieties of tomatoes such as Sour (Mallika local variety) raw (SrRT-SrRTPP, SrRTPO), Sour ripe (SrRIT-SrRITPP, SrRITPO), Sweet (Rashmi local variety) raw (SwRT-SwRTPP, SwRTPO) and Sweet ripe (SwRIT-SwRITPP and SwRITPO).
26939940	5	67	theme	free	1205:1208	arg1	FRS					1230:1232	FRS	1230:1232	FRS	1230:1232	The IC50 as determined by free radical scavenging (FRS), red blood cell (RBC) and DNA protection assays revealed reduction in FRS and RBC protective potencies in pectic oligosaccharides (POs) than pectic polysaccharides (PPs), supporting the fact that phenolics contribute towards these activities.
26939940	5	67	theme	free	1205:1208	arg1	scavenging					1218:1227	free radical scavenging	1205:1227	free radical scavenging (FRS)	1205:1233	The IC50 as determined by free radical scavenging (FRS), red blood cell (RBC) and DNA protection assays revealed reduction in FRS and RBC protective potencies in pectic oligosaccharides (POs) than pectic polysaccharides (PPs), supporting the fact that phenolics contribute towards these activities.
26939940	8	68	theme	sugar	1892:1896	arg1	composition					1898:1908	sugar composition	1892:1908	sugar composition that reflect changes in antioxidants	1892:1945	Precise alterations in biomolecular components, such as bound cinnamyl/ferulyl and vanillic acid derivatives, along with alterations in sugar composition that reflect changes in antioxidants, cyto/DNA protective and antimetastatic potentials, have been delineated.
26939940	0	69	dep	tomato	134:139	arg1	lycopersicum					150:161	Solanum lycopersicum	142:161	Solanum lycopersicum	142:161	Physiologically induced changes in bound phenolics and antioxidants, DNA/cytoprotective potentials in pectic poly/oligosaccharides of tomato (Solanum lycopersicum).
26939940	0	70	theme	tomato	134:139	arg1	poly/oligosaccharides					109:129	pectic poly/oligosaccharides	102:129	pectic poly/oligosaccharides of tomato (Solanum lycopersicum)	102:162	Physiologically induced changes in bound phenolics and antioxidants, DNA/cytoprotective potentials in pectic poly/oligosaccharides of tomato (Solanum lycopersicum).
26939940	3	71	theme	galectin-3	1025:1034	arg1	property					1047:1054	approximately four- to five-fold better galectin-3 inhibitory property	985:1054	approximately four- to five-fold better galectin-3 inhibitory property	985:1054	RESULTS Results indicate that unripe PPs and POs show approximately four- to five-fold better galectin-3 inhibitory property than ripe ones.
26939940	6	72	theme	certain	1536:1542	arg1	phenolics					1544:1552	certain phenolics	1536:1552	certain phenolics	1536:1552	Loss of activity could be attributed to the hydrolysis of certain phenolics during the ripening process as well as during conversion of PPs to POs.
26939940	0	73	theme	induced	16:22	arg1	changes					24:30	Physiologically induced changes	0:30	Physiologically induced changes	0:30	Physiologically induced changes in bound phenolics and antioxidants, DNA/cytoprotective potentials in pectic poly/oligosaccharides of tomato (Solanum lycopersicum).
26939940	3	74	theme	five-fold	1008:1016	arg1	property					1047:1054	approximately four- to five-fold better galectin-3 inhibitory property	985:1054	approximately four- to five-fold better galectin-3 inhibitory property	985:1054	RESULTS Results indicate that unripe PPs and POs show approximately four- to five-fold better galectin-3 inhibitory property than ripe ones.
26939940	8	75	from	alterations	1764:1774	arg1	cinnamyl/ferulyl					1818:1833	bound cinnamyl/ferulyl	1812:1833	bound cinnamyl/ferulyl	1812:1833	Precise alterations in biomolecular components, such as bound cinnamyl/ferulyl and vanillic acid derivatives, along with alterations in sugar composition that reflect changes in antioxidants, cyto/DNA protective and antimetastatic potentials, have been delineated.
26939940	8	75	from	alterations	1764:1774	arg1	components					1792:1801	biomolecular components	1779:1801	biomolecular components	1779:1801	Precise alterations in biomolecular components, such as bound cinnamyl/ferulyl and vanillic acid derivatives, along with alterations in sugar composition that reflect changes in antioxidants, cyto/DNA protective and antimetastatic potentials, have been delineated.
26939940	8	75	from	alterations	1764:1774	arg1	derivatives					1853:1863	vanillic acid derivatives	1839:1863	vanillic acid derivatives	1839:1863	Precise alterations in biomolecular components, such as bound cinnamyl/ferulyl and vanillic acid derivatives, along with alterations in sugar composition that reflect changes in antioxidants, cyto/DNA protective and antimetastatic potentials, have been delineated.
26939940	3	76	theme	ripe	1061:1064	arg1	ones					1066:1069	ripe ones	1061:1069	ripe ones	1061:1069	RESULTS Results indicate that unripe PPs and POs show approximately four- to five-fold better galectin-3 inhibitory property than ripe ones.
26939940	5	77	theme	blood	1240:1244	arg1	RBC					1252:1254	RBC	1252:1254	RBC	1252:1254	The IC50 as determined by free radical scavenging (FRS), red blood cell (RBC) and DNA protection assays revealed reduction in FRS and RBC protective potencies in pectic oligosaccharides (POs) than pectic polysaccharides (PPs), supporting the fact that phenolics contribute towards these activities.
26939940	5	77	theme	blood	1240:1244	arg1	cell					1246:1249	red blood cell	1236:1249	red blood cell (RBC)	1236:1255	The IC50 as determined by free radical scavenging (FRS), red blood cell (RBC) and DNA protection assays revealed reduction in FRS and RBC protective potencies in pectic oligosaccharides (POs) than pectic polysaccharides (PPs), supporting the fact that phenolics contribute towards these activities.
26939940	3	78	dep	five-fold	1008:1016	arg1	better					1018:1023	better	1018:1023	better	1018:1023	RESULTS Results indicate that unripe PPs and POs show approximately four- to five-fold better galectin-3 inhibitory property than ripe ones.
26939940	3	78	dep	five-fold	1008:1016	arg1	to					1005:1006	to	1005:1006	to	1005:1006	RESULTS Results indicate that unripe PPs and POs show approximately four- to five-fold better galectin-3 inhibitory property than ripe ones.
26939940	1	79	theme	cellular	322:329	arg1	damages					331:337	cellular damages	322:337	cellular damages	322:337	BACKGROUND Antioxidant, cyto/DNA protective potentials are known to offer significant protection against free radical induced injury to cells or tissues and cellular damages that are envisaged in various diseases including chronic diseases like cancer, diabetes, etc, while galectin-3 inhibitory potentials are known to block or delay the process of metastasis in cancer.
26939940	5	80	from	reduction	1292:1300	arg1	oligosaccharides					1348:1363	pectic oligosaccharides	1341:1363	pectic oligosaccharides (POs) than pectic polysaccharides (PPs)	1341:1403	The IC50 as determined by free radical scavenging (FRS), red blood cell (RBC) and DNA protection assays revealed reduction in FRS and RBC protective potencies in pectic oligosaccharides (POs) than pectic polysaccharides (PPs), supporting the fact that phenolics contribute towards these activities.
26939940	5	80	from	reduction	1292:1300	arg1	RBC					1313:1315	RBC	1313:1315	RBC	1313:1315	The IC50 as determined by free radical scavenging (FRS), red blood cell (RBC) and DNA protection assays revealed reduction in FRS and RBC protective potencies in pectic oligosaccharides (POs) than pectic polysaccharides (PPs), supporting the fact that phenolics contribute towards these activities.
26939940	5	80	from	reduction	1292:1300	arg1	FRS					1305:1307	FRS	1305:1307	FRS	1305:1307	The IC50 as determined by free radical scavenging (FRS), red blood cell (RBC) and DNA protection assays revealed reduction in FRS and RBC protective potencies in pectic oligosaccharides (POs) than pectic polysaccharides (PPs), supporting the fact that phenolics contribute towards these activities.
26939940	5	80	from	reduction	1292:1300	arg1	POs					1366:1368	POs	1366:1368	POs	1366:1368	The IC50 as determined by free radical scavenging (FRS), red blood cell (RBC) and DNA protection assays revealed reduction in FRS and RBC protective potencies in pectic oligosaccharides (POs) than pectic polysaccharides (PPs), supporting the fact that phenolics contribute towards these activities.
26939940	8	81	theme	cyto/DNA	1948:1955	arg1	potentials					1987:1996	cyto/DNA protective and antimetastatic potentials	1948:1996	cyto/DNA protective and antimetastatic potentials	1948:1996	Precise alterations in biomolecular components, such as bound cinnamyl/ferulyl and vanillic acid derivatives, along with alterations in sugar composition that reflect changes in antioxidants, cyto/DNA protective and antimetastatic potentials, have been delineated.
26939940	2	82	dep	raw	769:771	arg1	SwRTPO					879:884	SwRTPO	879:884	SwRTPO	879:884	Antioxidant, cyto/DNA protection and galectin-3 inhibitory potentials were examined in pectic polysaccharides (PPs) and pectic oligosaccharides (POs) from four types of two varieties of tomatoes such as Sour (Mallika local variety) raw (SrRT-SrRTPP, SrRTPO), Sour ripe (SrRIT-SrRITPP, SrRITPO), Sweet (Rashmi local variety) raw (SwRT-SwRTPP, SwRTPO) and Sweet ripe (SwRIT-SwRITPP and SwRITPO).
26939940	2	82	dep	raw	769:771	arg1	ripe					897:900	raw (SwRT-SwRTPP, SwRTPO) and Sweet ripe	861:900	ripe	897:900	Antioxidant, cyto/DNA protection and galectin-3 inhibitory potentials were examined in pectic polysaccharides (PPs) and pectic oligosaccharides (POs) from four types of two varieties of tomatoes such as Sour (Mallika local variety) raw (SrRT-SrRTPP, SrRTPO), Sour ripe (SrRIT-SrRITPP, SrRITPO), Sweet (Rashmi local variety) raw (SwRT-SwRTPP, SwRTPO) and Sweet ripe (SwRIT-SwRITPP and SwRITPO).
26939940	2	82	dep	raw	769:771	arg1	raw					861:863	raw (SwRT-SwRTPP, SwRTPO) and Sweet ripe	861:900	raw	861:863	Antioxidant, cyto/DNA protection and galectin-3 inhibitory potentials were examined in pectic polysaccharides (PPs) and pectic oligosaccharides (POs) from four types of two varieties of tomatoes such as Sour (Mallika local variety) raw (SrRT-SrRTPP, SrRTPO), Sour ripe (SrRIT-SrRITPP, SrRITPO), Sweet (Rashmi local variety) raw (SwRT-SwRTPP, SwRTPO) and Sweet ripe (SwRIT-SwRITPP and SwRITPO).
26939940	1	83	theme	metastasis	515:524	arg1	process					504:510	the process	500:510	the process of metastasis in cancer	500:534	BACKGROUND Antioxidant, cyto/DNA protective potentials are known to offer significant protection against free radical induced injury to cells or tissues and cellular damages that are envisaged in various diseases including chronic diseases like cancer, diabetes, etc, while galectin-3 inhibitory potentials are known to block or delay the process of metastasis in cancer.
26939940	7	84	theme	ripened	1726:1732	arg1	conditions					1734:1743	unripened and ripened conditions	1712:1743	unripened and ripened conditions in tomato	1712:1753	CONCLUSION This study, for the first time, showed changes in bioactivity profiling in unripened and ripened conditions in tomato.
26939940	4	85	theme	inhibitory	1129:1138	arg1	activity					1140:1147	galectin-3 inhibitory activity	1118:1147	galectin-3 inhibitory activity in sour variety	1118:1163	An approximately nine- to 10-fold increase in galectin-3 inhibitory activity in sour variety was observed.
26939940	5	86	theme	DNA	1261:1263	arg1	assays					1276:1281	DNA protection assays	1261:1281	DNA protection assays	1261:1281	The IC50 as determined by free radical scavenging (FRS), red blood cell (RBC) and DNA protection assays revealed reduction in FRS and RBC protective potencies in pectic oligosaccharides (POs) than pectic polysaccharides (PPs), supporting the fact that phenolics contribute towards these activities.
26939940	7	87	theme	unripened	1712:1720	arg1	conditions					1734:1743	unripened and ripened conditions	1712:1743	unripened and ripened conditions in tomato	1712:1753	CONCLUSION This study, for the first time, showed changes in bioactivity profiling in unripened and ripened conditions in tomato.
28867226	0	0	theme	ascidian	65:72	arg1	exasperatus					86:96	the ascidian Microcosmus exasperatus	61:96	the ascidian Microcosmus exasperatus	61:96	Structural analysis of a sulfated galactan from the tunic of the ascidian Microcosmus exasperatus and its inhibitory effect of the intrinsic coagulation pathway.
28867226	8	1	attach	presents	1248:1255	arg2	polysaccharide					1183:1196	This sulfated polysaccharide	1169:1196	This sulfated polysaccharide	1169:1196	This sulfated polysaccharide, which has an average molecular mass of 439.5kDa, presents anticoagulant effect in a dose-dependent manner through the inhibition of the intrinsic coagulation pathway.
28867226	8	1	attach	presents	1248:1255	arg1	manner					1298:1303	a dose-dependent manner	1281:1303	a dose-dependent manner	1281:1303	This sulfated polysaccharide, which has an average molecular mass of 439.5kDa, presents anticoagulant effect in a dose-dependent manner through the inhibition of the intrinsic coagulation pathway.
28867226	8	2	theme	sulfated	1174:1181	arg1	polysaccharide					1183:1196	This sulfated polysaccharide	1169:1196	This sulfated polysaccharide	1169:1196	This sulfated polysaccharide, which has an average molecular mass of 439.5kDa, presents anticoagulant effect in a dose-dependent manner through the inhibition of the intrinsic coagulation pathway.
28867226	1	3	theme	species	246:252	arg1	tunic					227:231	the tunic	223:231	the tunic of different species of ascidians	223:265	Several bioactive sulfated galactans have been isolated from the tunic of different species of ascidians.
28867226	0	4	from	analysis	11:18	arg1	tunic					52:56	the tunic	48:56	the tunic of the ascidian Microcosmus exasperatus	48:96	Structural analysis of a sulfated galactan from the tunic of the ascidian Microcosmus exasperatus and its inhibitory effect of the intrinsic coagulation pathway.
28867226	3	5	theme	freeze-thaw	629:639	arg1	cycles					641:646	freeze-thaw cycles	629:646	freeze-thaw cycles	629:646	A sulfated galactan (1000RS) was isolated from the tunic of the Brazilian ascidia Microcosmus exasperatus through proteolytic digestion, ethanol precipitation, dialysis and freeze-thaw cycles.
28867226	0	6	theme	exasperatus	86:96	arg1	tunic					52:56	the tunic	48:56	the tunic of the ascidian Microcosmus exasperatus	48:96	Structural analysis of a sulfated galactan from the tunic of the ascidian Microcosmus exasperatus and its inhibitory effect of the intrinsic coagulation pathway.
28867226	2	7	dep	presence	350:357	arg1	the					346:348	the	346:348	the	346:348	The biological activity of this kind of polysaccharides has been related with the presence and position of sulfate groups, and by the chemical composition of this kind of polysaccharides.
28867226	1	8	theme	ascidians	257:265	arg1	species					246:252	different species	236:252	different species of ascidians	236:265	Several bioactive sulfated galactans have been isolated from the tunic of different species of ascidians.
28867226	0	9	theme	Microcosmus	74:84	arg1	exasperatus					86:96	the ascidian Microcosmus exasperatus	61:96	the ascidian Microcosmus exasperatus	61:96	Structural analysis of a sulfated galactan from the tunic of the ascidian Microcosmus exasperatus and its inhibitory effect of the intrinsic coagulation pathway.
28867226	6	10	link	4-linked	1035:1042	arg1	units					1053:1057	4-linked α-l-Galp units	1035:1057	4-linked α-l-Galp units	1035:1057	These experiments along with NMR and FTIR analysis allowed to claim that the galactan backbone is mainly composed of 4-linked α-l-Galp units.
28867226	2	11	theme	biological	272:281	arg1	activity					283:290	The biological activity	268:290	The biological activity of this kind of polysaccharides	268:322	The biological activity of this kind of polysaccharides has been related with the presence and position of sulfate groups, and by the chemical composition of this kind of polysaccharides.
28867226	2	12	theme	sulfate	375:381	arg1	groups					383:388	sulfate groups	375:388	sulfate groups	375:388	The biological activity of this kind of polysaccharides has been related with the presence and position of sulfate groups, and by the chemical composition of this kind of polysaccharides.
28867226	1	13	theme	Several	162:168	arg1	galactans					189:197	Several bioactive sulfated galactans	162:197	Several bioactive sulfated galactans	162:197	Several bioactive sulfated galactans have been isolated from the tunic of different species of ascidians.
28867226	7	14	theme	galactose	1118:1126	arg1	residues					1128:1135	the galactose residues	1114:1135	the galactose residues	1114:1135	In addition, they permitted to establish that some of the galactose residues are sulfated at the 3-position.
28867226	8	15	contain	has	1205:1207	arg1	polysaccharide					1183:1196	This sulfated polysaccharide	1169:1196	This sulfated polysaccharide	1169:1196	This sulfated polysaccharide, which has an average molecular mass of 439.5kDa, presents anticoagulant effect in a dose-dependent manner through the inhibition of the intrinsic coagulation pathway.
28867226	8	15	contain	has	1205:1207	arg2	mass					1230:1233	an average molecular mass	1209:1233	an average molecular mass of 439.5kDa	1209:1245	This sulfated polysaccharide, which has an average molecular mass of 439.5kDa, presents anticoagulant effect in a dose-dependent manner through the inhibition of the intrinsic coagulation pathway.
28867226	5	16	theme	mass	831:834	arg1	spectrometry					836:847	mass spectrometry	831:847	mass spectrometry	831:847	Monosaccharide composition and type of linkage were assessed by Gas chromatography coupled to mass spectrometry and the sulfate content was quantified through gelatin/BaCl2 method.
28867226	1	17	theme	bioactive	170:178	arg1	galactans					189:197	Several bioactive sulfated galactans	162:197	Several bioactive sulfated galactans	162:197	Several bioactive sulfated galactans have been isolated from the tunic of different species of ascidians.
28867226	2	18	theme	kind	431:434	arg1	composition					411:421	the chemical composition	398:421	the chemical composition of this kind of polysaccharides	398:453	The biological activity of this kind of polysaccharides has been related with the presence and position of sulfate groups, and by the chemical composition of this kind of polysaccharides.
28867226	2	19	theme	polysaccharides	308:322	arg1	kind					300:303	this kind	295:303	this kind of polysaccharides	295:322	The biological activity of this kind of polysaccharides has been related with the presence and position of sulfate groups, and by the chemical composition of this kind of polysaccharides.
28867226	1	20	theme	sulfated	180:187	arg1	galactans					189:197	Several bioactive sulfated galactans	162:197	Several bioactive sulfated galactans	162:197	Several bioactive sulfated galactans have been isolated from the tunic of different species of ascidians.
28867226	0	21	theme	Structural	0:9	arg1	analysis					11:18	Structural analysis	0:18	Structural analysis of a sulfated galactan from the tunic of the ascidian Microcosmus exasperatus and its inhibitory effect of the intrinsic coagulation pathway.	0:160	Structural analysis of a sulfated galactan from the tunic of the ascidian Microcosmus exasperatus and its inhibitory effect of the intrinsic coagulation pathway.
28867226	8	22	theme	anticoagulant	1257:1269	arg1	effect					1271:1276	anticoagulant effect	1257:1276	anticoagulant effect	1257:1276	This sulfated polysaccharide, which has an average molecular mass of 439.5kDa, presents anticoagulant effect in a dose-dependent manner through the inhibition of the intrinsic coagulation pathway.
28867226	5	23	theme	Monosaccharide	737:750	arg1	composition					752:762	Monosaccharide composition	737:762	Monosaccharide composition	737:762	Monosaccharide composition and type of linkage were assessed by Gas chromatography coupled to mass spectrometry and the sulfate content was quantified through gelatin/BaCl2 method.
28867226	4	24	theme	molecular	665:673	arg1	weight					675:680	molecular weight	665:680	molecular weight	665:680	Homogeneity and molecular weight were estimated by using size exclusion chromatography.
28867226	8	25	theme	molecular	1220:1228	arg1	mass					1230:1233	an average molecular mass	1209:1233	an average molecular mass of 439.5kDa	1209:1245	This sulfated polysaccharide, which has an average molecular mass of 439.5kDa, presents anticoagulant effect in a dose-dependent manner through the inhibition of the intrinsic coagulation pathway.
28867226	6	26	theme	galactan	995:1002	arg1	backbone					1004:1011	the galactan backbone	991:1011	the galactan backbone	991:1011	These experiments along with NMR and FTIR analysis allowed to claim that the galactan backbone is mainly composed of 4-linked α-l-Galp units.
28867226	0	27	theme	effect	117:122	arg1	analysis					11:18	Structural analysis	0:18	Structural analysis of a sulfated galactan from the tunic of the ascidian Microcosmus exasperatus and its inhibitory effect of the intrinsic coagulation pathway.	0:160	Structural analysis of a sulfated galactan from the tunic of the ascidian Microcosmus exasperatus and its inhibitory effect of the intrinsic coagulation pathway.
28867226	2	28	theme	kind	300:303	arg1	activity					283:290	The biological activity	268:290	The biological activity of this kind of polysaccharides	268:322	The biological activity of this kind of polysaccharides has been related with the presence and position of sulfate groups, and by the chemical composition of this kind of polysaccharides.
28867226	2	29	theme	chemical	402:409	arg1	composition					411:421	the chemical composition	398:421	the chemical composition of this kind of polysaccharides	398:453	The biological activity of this kind of polysaccharides has been related with the presence and position of sulfate groups, and by the chemical composition of this kind of polysaccharides.
28867226	8	30	theme	average	1212:1218	arg1	mass					1230:1233	an average molecular mass	1209:1233	an average molecular mass of 439.5kDa	1209:1245	This sulfated polysaccharide, which has an average molecular mass of 439.5kDa, presents anticoagulant effect in a dose-dependent manner through the inhibition of the intrinsic coagulation pathway.
28867226	0	31	theme	inhibitory	106:115	arg1	effect					117:122	its inhibitory effect	102:122	its inhibitory effect of the intrinsic coagulation pathway	102:159	Structural analysis of a sulfated galactan from the tunic of the ascidian Microcosmus exasperatus and its inhibitory effect of the intrinsic coagulation pathway.
28867226	3	32	theme	ascidia	530:536	arg1	tunic					507:511	the tunic	503:511	the tunic of the Brazilian ascidia	503:536	A sulfated galactan (1000RS) was isolated from the tunic of the Brazilian ascidia Microcosmus exasperatus through proteolytic digestion, ethanol precipitation, dialysis and freeze-thaw cycles.
28867226	5	33	theme	sulfate	857:863	arg1	content					865:871	the sulfate content	853:871	the sulfate content	853:871	Monosaccharide composition and type of linkage were assessed by Gas chromatography coupled to mass spectrometry and the sulfate content was quantified through gelatin/BaCl2 method.
28867226	0	34	theme	galactan	34:41	arg1	analysis					11:18	Structural analysis	0:18	Structural analysis of a sulfated galactan from the tunic of the ascidian Microcosmus exasperatus and its inhibitory effect of the intrinsic coagulation pathway.	0:160	Structural analysis of a sulfated galactan from the tunic of the ascidian Microcosmus exasperatus and its inhibitory effect of the intrinsic coagulation pathway.
28867226	8	35	theme	pathway	1357:1363	arg1	inhibition					1317:1326	the inhibition	1313:1326	the inhibition of the intrinsic coagulation pathway	1313:1363	This sulfated polysaccharide, which has an average molecular mass of 439.5kDa, presents anticoagulant effect in a dose-dependent manner through the inhibition of the intrinsic coagulation pathway.
28867226	0	36	theme	sulfated	25:32	arg1	galactan					34:41	a sulfated galactan	23:41	a sulfated galactan from the tunic of the ascidian Microcosmus exasperatus	23:96	Structural analysis of a sulfated galactan from the tunic of the ascidian Microcosmus exasperatus and its inhibitory effect of the intrinsic coagulation pathway.
28867226	5	37	theme	Gas	801:803	arg1	chromatography					805:818	Gas chromatography	801:818	Gas chromatography coupled to mass spectrometry	801:847	Monosaccharide composition and type of linkage were assessed by Gas chromatography coupled to mass spectrometry and the sulfate content was quantified through gelatin/BaCl2 method.
28867226	2	38	theme	polysaccharides	439:453	arg1	kind					431:434	this kind	426:434	this kind of polysaccharides	426:453	The biological activity of this kind of polysaccharides has been related with the presence and position of sulfate groups, and by the chemical composition of this kind of polysaccharides.
28867226	5	39	theme	gelatin/BaCl2	896:908	arg1	method					910:915	gelatin/BaCl2 method	896:915	gelatin/BaCl2 method	896:915	Monosaccharide composition and type of linkage were assessed by Gas chromatography coupled to mass spectrometry and the sulfate content was quantified through gelatin/BaCl2 method.
28867226	0	40	theme	coagulation	141:151	arg1	pathway					153:159	the intrinsic coagulation pathway	127:159	the intrinsic coagulation pathway	127:159	Structural analysis of a sulfated galactan from the tunic of the ascidian Microcosmus exasperatus and its inhibitory effect of the intrinsic coagulation pathway.
28867226	0	41	from	tunic	52:56	arg1	galactan					34:41	a sulfated galactan	23:41	a sulfated galactan from the tunic of the ascidian Microcosmus exasperatus	23:96	Structural analysis of a sulfated galactan from the tunic of the ascidian Microcosmus exasperatus and its inhibitory effect of the intrinsic coagulation pathway.
28867226	0	41	from	tunic	52:56	arg1	analysis					11:18	Structural analysis	0:18	Structural analysis of a sulfated galactan from the tunic of the ascidian Microcosmus exasperatus and its inhibitory effect of the intrinsic coagulation pathway.	0:160	Structural analysis of a sulfated galactan from the tunic of the ascidian Microcosmus exasperatus and its inhibitory effect of the intrinsic coagulation pathway.
28867226	0	41	from	tunic	52:56	arg1	effect					117:122	its inhibitory effect	102:122	its inhibitory effect of the intrinsic coagulation pathway	102:159	Structural analysis of a sulfated galactan from the tunic of the ascidian Microcosmus exasperatus and its inhibitory effect of the intrinsic coagulation pathway.
28867226	3	42	attach	isolated	489:496	arg2	1000RS					477:482	1000RS	477:482	1000RS	477:482	A sulfated galactan (1000RS) was isolated from the tunic of the Brazilian ascidia Microcosmus exasperatus through proteolytic digestion, ethanol precipitation, dialysis and freeze-thaw cycles.
28867226	3	42	attach	isolated	489:496	arg1	tunic					507:511	the tunic	503:511	the tunic of the Brazilian ascidia	503:536	A sulfated galactan (1000RS) was isolated from the tunic of the Brazilian ascidia Microcosmus exasperatus through proteolytic digestion, ethanol precipitation, dialysis and freeze-thaw cycles.
28867226	3	42	attach	isolated	489:496	arg2	galactan					467:474	A sulfated galactan	456:474	A sulfated galactan (1000RS)	456:483	A sulfated galactan (1000RS) was isolated from the tunic of the Brazilian ascidia Microcosmus exasperatus through proteolytic digestion, ethanol precipitation, dialysis and freeze-thaw cycles.
28867226	4	43	theme	exclusion	711:719	arg1	chromatography					721:734	size exclusion chromatography	706:734	size exclusion chromatography	706:734	Homogeneity and molecular weight were estimated by using size exclusion chromatography.
28867226	8	44	theme	439.5kDa	1238:1245	arg1	mass					1230:1233	an average molecular mass	1209:1233	an average molecular mass of 439.5kDa	1209:1245	This sulfated polysaccharide, which has an average molecular mass of 439.5kDa, presents anticoagulant effect in a dose-dependent manner through the inhibition of the intrinsic coagulation pathway.
28867226	0	45	theme	intrinsic	131:139	arg1	pathway					153:159	the intrinsic coagulation pathway	127:159	the intrinsic coagulation pathway	127:159	Structural analysis of a sulfated galactan from the tunic of the ascidian Microcosmus exasperatus and its inhibitory effect of the intrinsic coagulation pathway.
28867226	3	46	theme	proteolytic	570:580	arg1	digestion					582:590	proteolytic digestion	570:590	proteolytic digestion	570:590	A sulfated galactan (1000RS) was isolated from the tunic of the Brazilian ascidia Microcosmus exasperatus through proteolytic digestion, ethanol precipitation, dialysis and freeze-thaw cycles.
28867226	4	47	theme	size	706:709	arg1	chromatography					721:734	size exclusion chromatography	706:734	size exclusion chromatography	706:734	Homogeneity and molecular weight were estimated by using size exclusion chromatography.
28867226	3	48	theme	sulfated	458:465	arg1	1000RS					477:482	1000RS	477:482	1000RS	477:482	A sulfated galactan (1000RS) was isolated from the tunic of the Brazilian ascidia Microcosmus exasperatus through proteolytic digestion, ethanol precipitation, dialysis and freeze-thaw cycles.
28867226	3	48	theme	sulfated	458:465	arg1	galactan					467:474	A sulfated galactan	456:474	A sulfated galactan (1000RS)	456:483	A sulfated galactan (1000RS) was isolated from the tunic of the Brazilian ascidia Microcosmus exasperatus through proteolytic digestion, ethanol precipitation, dialysis and freeze-thaw cycles.
28867226	3	49	theme	Brazilian	520:528	arg1	ascidia					530:536	the Brazilian ascidia	516:536	the Brazilian ascidia	516:536	A sulfated galactan (1000RS) was isolated from the tunic of the Brazilian ascidia Microcosmus exasperatus through proteolytic digestion, ethanol precipitation, dialysis and freeze-thaw cycles.
28867226	0	50	theme	pathway	153:159	arg1	galactan					34:41	a sulfated galactan	23:41	a sulfated galactan from the tunic of the ascidian Microcosmus exasperatus	23:96	Structural analysis of a sulfated galactan from the tunic of the ascidian Microcosmus exasperatus and its inhibitory effect of the intrinsic coagulation pathway.
28867226	0	50	theme	pathway	153:159	arg1	effect					117:122	its inhibitory effect	102:122	its inhibitory effect of the intrinsic coagulation pathway	102:159	Structural analysis of a sulfated galactan from the tunic of the ascidian Microcosmus exasperatus and its inhibitory effect of the intrinsic coagulation pathway.
28867226	1	51	attach	isolated	209:216	arg2	galactans					189:197	Several bioactive sulfated galactans	162:197	Several bioactive sulfated galactans	162:197	Several bioactive sulfated galactans have been isolated from the tunic of different species of ascidians.
28867226	1	51	attach	isolated	209:216	arg1	tunic					227:231	the tunic	223:231	the tunic of different species of ascidians	223:265	Several bioactive sulfated galactans have been isolated from the tunic of different species of ascidians.
28867226	3	52	theme	ethanol	593:599	arg1	precipitation					601:613	ethanol precipitation	593:613	ethanol precipitation	593:613	A sulfated galactan (1000RS) was isolated from the tunic of the Brazilian ascidia Microcosmus exasperatus through proteolytic digestion, ethanol precipitation, dialysis and freeze-thaw cycles.
28867226	8	53	theme	dose-dependent	1283:1296	arg1	manner					1298:1303	a dose-dependent manner	1281:1303	a dose-dependent manner	1281:1303	This sulfated polysaccharide, which has an average molecular mass of 439.5kDa, presents anticoagulant effect in a dose-dependent manner through the inhibition of the intrinsic coagulation pathway.
28867226	8	54	theme	coagulation	1345:1355	arg1	pathway					1357:1363	the intrinsic coagulation pathway	1331:1363	the intrinsic coagulation pathway	1331:1363	This sulfated polysaccharide, which has an average molecular mass of 439.5kDa, presents anticoagulant effect in a dose-dependent manner through the inhibition of the intrinsic coagulation pathway.
28867226	2	55	theme	groups	383:388	arg1	presence					350:357	presence	350:357	presence	350:357	The biological activity of this kind of polysaccharides has been related with the presence and position of sulfate groups, and by the chemical composition of this kind of polysaccharides.
28867226	6	56	theme	α-l-Galp	1044:1051	arg1	units					1053:1057	4-linked α-l-Galp units	1035:1057	4-linked α-l-Galp units	1035:1057	These experiments along with NMR and FTIR analysis allowed to claim that the galactan backbone is mainly composed of 4-linked α-l-Galp units.
28867226	5	57	theme	linkage	776:782	arg1	type					768:771	type	768:771	type	768:771	Monosaccharide composition and type of linkage were assessed by Gas chromatography coupled to mass spectrometry and the sulfate content was quantified through gelatin/BaCl2 method.
28867226	5	57	theme	linkage	776:782	arg1	composition					752:762	Monosaccharide composition	737:762	Monosaccharide composition	737:762	Monosaccharide composition and type of linkage were assessed by Gas chromatography coupled to mass spectrometry and the sulfate content was quantified through gelatin/BaCl2 method.
28867226	6	58	theme	FTIR	955:958	arg1	analysis					960:967	FTIR analysis	955:967	FTIR analysis	955:967	These experiments along with NMR and FTIR analysis allowed to claim that the galactan backbone is mainly composed of 4-linked α-l-Galp units.
28867226	6	59	theme	4-linked	1035:1042	arg1	units					1053:1057	4-linked α-l-Galp units	1035:1057	4-linked α-l-Galp units	1035:1057	These experiments along with NMR and FTIR analysis allowed to claim that the galactan backbone is mainly composed of 4-linked α-l-Galp units.
28867226	8	60	theme	intrinsic	1335:1343	arg1	pathway					1357:1363	the intrinsic coagulation pathway	1331:1363	the intrinsic coagulation pathway	1331:1363	This sulfated polysaccharide, which has an average molecular mass of 439.5kDa, presents anticoagulant effect in a dose-dependent manner through the inhibition of the intrinsic coagulation pathway.
28867226	1	61	theme	different	236:244	arg1	species					246:252	different species	236:252	different species of ascidians	236:265	Several bioactive sulfated galactans have been isolated from the tunic of different species of ascidians.
25896375	3	0	with	system	683:688	arg1	chambers					706:713	individual chambers	695:713	individual chambers	695:713	Two key advances have been made that facilitate cultivation of switchgrass, an important forage and biofuel crop, for controlled isotopic enrichment: (1) perfusion system with individual chambers and (2) hydroponic growth from tiller cuttings.
25896375	9	1	theme	NMR	1547:1549	arg1	techniques					1551:1560	NMR techniques	1547:1560	NMR techniques	1547:1560	This capability to produce deuterated lignocellulosic biomass under controlled conditions will enhance investigation of cell wall structure and its deconstruction by neutron scattering and NMR techniques.
25896375	1	2	theme	H	257:257	arg1	controls					281:288	H 2 O-grown switchgrass controls	257:288	H 2 O-grown switchgrass controls	257:288	The bioenergy crop switchgrass was grown hydroponically from tiller cuttings in 50 % D 2 O to obtain biomass with 34 % deuterium substitution and physicochemical properties similar to those of H 2 O-grown switchgrass controls.
25896375	4	3	theme	periodic	820:827	arg1	harvest					829:835	periodic harvest	820:835	periodic harvest	820:835	Plants were grown and maintained for several months with periodic harvest.
25896375	3	4	theme	individual	695:704	arg1	chambers					706:713	individual chambers	695:713	individual chambers	695:713	Two key advances have been made that facilitate cultivation of switchgrass, an important forage and biofuel crop, for controlled isotopic enrichment: (1) perfusion system with individual chambers and (2) hydroponic growth from tiller cuttings.
25896375	9	5	theme	lignocellulosic	1396:1410	arg1	biomass					1412:1418	deuterated lignocellulosic biomass	1385:1418	deuterated lignocellulosic biomass	1385:1418	This capability to produce deuterated lignocellulosic biomass under controlled conditions will enhance investigation of cell wall structure and its deconstruction by neutron scattering and NMR techniques.
25896375	3	6	theme	hydroponic	723:732	arg1	growth					734:739	hydroponic growth	723:739	(2) hydroponic growth from tiller cuttings	719:760	Two key advances have been made that facilitate cultivation of switchgrass, an important forage and biofuel crop, for controlled isotopic enrichment: (1) perfusion system with individual chambers and (2) hydroponic growth from tiller cuttings.
25896375	7	7	theme	switchgrass	1076:1086	arg1	quantities					1062:1071	gram quantities	1057:1071	gram quantities of switchgrass leaves and stems	1057:1103	Using this improved method, gram quantities of switchgrass leaves and stems were produced by continuous hydroponic cultivation using growth medium consisting of basal mineral salts in 50 % D2O.
25896375	3	8	dep	important	598:606	arg1	forage					608:613	forage	608:613	forage	608:613	Two key advances have been made that facilitate cultivation of switchgrass, an important forage and biofuel crop, for controlled isotopic enrichment: (1) perfusion system with individual chambers and (2) hydroponic growth from tiller cuttings.
25896375	1	9	theme	%	181:181	arg1	substitution					193:204	34 % deuterium substitution	178:204	34 % deuterium substitution	178:204	The bioenergy crop switchgrass was grown hydroponically from tiller cuttings in 50 % D 2 O to obtain biomass with 34 % deuterium substitution and physicochemical properties similar to those of H 2 O-grown switchgrass controls.
25896375	7	10	dep	switchgrass	1076:1086	arg1	leaves					1088:1093	leaves	1088:1093	leaves	1088:1093	Using this improved method, gram quantities of switchgrass leaves and stems were produced by continuous hydroponic cultivation using growth medium consisting of basal mineral salts in 50 % D2O.
25896375	7	11	theme	growth	1162:1167	arg1	medium					1169:1174	growth medium	1162:1174	growth medium consisting of basal mineral salts in 50 % D2O	1162:1220	Using this improved method, gram quantities of switchgrass leaves and stems were produced by continuous hydroponic cultivation using growth medium consisting of basal mineral salts in 50 % D2O.
25896375	7	12	theme	improved	1040:1047	arg1	method					1049:1054	this improved method	1035:1054	this improved method	1035:1054	Using this improved method, gram quantities of switchgrass leaves and stems were produced by continuous hydroponic cultivation using growth medium consisting of basal mineral salts in 50 % D2O.
25896375	1	13	from	cuttings	132:139	arg1	O					153:153	50 % D 2 O	144:153	50 % D 2 O	144:153	The bioenergy crop switchgrass was grown hydroponically from tiller cuttings in 50 % D 2 O to obtain biomass with 34 % deuterium substitution and physicochemical properties similar to those of H 2 O-grown switchgrass controls.
25896375	8	14	theme	stretching	1293:1302	arg1	peaks					1304:1308	the O-D and C-D stretching peaks	1277:1308	peaks	1304:1308	Deuterium incorporation was confirmed by detection of the O-D and C-D stretching peaks with FTIR and quantified by (1)H- and (2)H-NMR.
25896375	4	15	with	months	808:813	arg1	harvest					829:835	periodic harvest	820:835	periodic harvest	820:835	Plants were grown and maintained for several months with periodic harvest.
25896375	9	16	theme	wall	1483:1486	arg1	structure					1488:1496	cell wall structure	1478:1496	cell wall structure	1478:1496	This capability to produce deuterated lignocellulosic biomass under controlled conditions will enhance investigation of cell wall structure and its deconstruction by neutron scattering and NMR techniques.
25896375	2	17	theme	cell	507:510	arg1	walls					512:516	plant cell walls	501:516	plant cell walls	501:516	Deuterium enrichment of biological materials can potentially enable expanded experimental use of small angle neutron scattering (SANS) to investigate molecular structural transitions of complex systems such as plant cell walls.
25896375	8	18	dep	H-NMR	1351:1355	arg1	2					1349:1349	2	1349:1349	2	1349:1349	Deuterium incorporation was confirmed by detection of the O-D and C-D stretching peaks with FTIR and quantified by (1)H- and (2)H-NMR.
25896375	1	19	theme	switchgrass	269:279	arg1	controls					281:288	H 2 O-grown switchgrass controls	257:288	H 2 O-grown switchgrass controls	257:288	The bioenergy crop switchgrass was grown hydroponically from tiller cuttings in 50 % D 2 O to obtain biomass with 34 % deuterium substitution and physicochemical properties similar to those of H 2 O-grown switchgrass controls.
25896375	7	20	theme	%	1216:1216	arg1	D2O					1218:1220	50 % D2O	1213:1220	50 % D2O	1213:1220	Using this improved method, gram quantities of switchgrass leaves and stems were produced by continuous hydroponic cultivation using growth medium consisting of basal mineral salts in 50 % D2O.
25896375	5	21	theme	CO2	894:896	arg1	measurement					879:889	measurement	879:889	measurement of CO2 in outflow from the growth chambers	879:932	Photosynthetic activity was monitored by measurement of CO2 in outflow from the growth chambers.
25896375	3	22	from	cuttings	753:760	arg1	system					683:688	perfusion system	673:688	(1) perfusion system with individual chambers	669:713	Two key advances have been made that facilitate cultivation of switchgrass, an important forage and biofuel crop, for controlled isotopic enrichment: (1) perfusion system with individual chambers and (2) hydroponic growth from tiller cuttings.
25896375	3	22	from	cuttings	753:760	arg1	growth					734:739	hydroponic growth	723:739	(2) hydroponic growth from tiller cuttings	719:760	Two key advances have been made that facilitate cultivation of switchgrass, an important forage and biofuel crop, for controlled isotopic enrichment: (1) perfusion system with individual chambers and (2) hydroponic growth from tiller cuttings.
25896375	9	23	theme	neutron	1524:1530	arg1	scattering					1532:1541	neutron scattering	1524:1541	neutron scattering	1524:1541	This capability to produce deuterated lignocellulosic biomass under controlled conditions will enhance investigation of cell wall structure and its deconstruction by neutron scattering and NMR techniques.
25896375	1	24	theme	50	144:145	arg1	%					147:147	%	147:147	%	147:147	The bioenergy crop switchgrass was grown hydroponically from tiller cuttings in 50 % D 2 O to obtain biomass with 34 % deuterium substitution and physicochemical properties similar to those of H 2 O-grown switchgrass controls.
25896375	2	25	theme	neutron	400:406	arg1	SANS					420:423	SANS	420:423	SANS	420:423	Deuterium enrichment of biological materials can potentially enable expanded experimental use of small angle neutron scattering (SANS) to investigate molecular structural transitions of complex systems such as plant cell walls.
25896375	2	25	theme	neutron	400:406	arg1	scattering					408:417	small angle neutron scattering	388:417	small angle neutron scattering (SANS)	388:424	Deuterium enrichment of biological materials can potentially enable expanded experimental use of small angle neutron scattering (SANS) to investigate molecular structural transitions of complex systems such as plant cell walls.
25896375	7	26	theme	mineral	1196:1202	arg1	salts					1204:1208	basal mineral salts	1190:1208	basal mineral salts in 50 % D2O	1190:1220	Using this improved method, gram quantities of switchgrass leaves and stems were produced by continuous hydroponic cultivation using growth medium consisting of basal mineral salts in 50 % D2O.
25896375	2	27	theme	structural	451:460	arg1	transitions					462:472	molecular structural transitions	441:472	molecular structural transitions of complex systems such as plant cell walls	441:516	Deuterium enrichment of biological materials can potentially enable expanded experimental use of small angle neutron scattering (SANS) to investigate molecular structural transitions of complex systems such as plant cell walls.
25896375	2	28	theme	small	388:392	arg1	SANS					420:423	SANS	420:423	SANS	420:423	Deuterium enrichment of biological materials can potentially enable expanded experimental use of small angle neutron scattering (SANS) to investigate molecular structural transitions of complex systems such as plant cell walls.
25896375	2	28	theme	small	388:392	arg1	scattering					408:417	small angle neutron scattering	388:417	small angle neutron scattering (SANS)	388:424	Deuterium enrichment of biological materials can potentially enable expanded experimental use of small angle neutron scattering (SANS) to investigate molecular structural transitions of complex systems such as plant cell walls.
25896375	0	29	theme	deuterated	14:23	arg1	switchgrass					25:35	deuterated switchgrass	14:35	deuterated switchgrass	14:35	Production of deuterated switchgrass by hydroponic cultivation.
25896375	3	30	dep	system	683:688	arg1	1					670:670	1	670:670	1	670:670	Two key advances have been made that facilitate cultivation of switchgrass, an important forage and biofuel crop, for controlled isotopic enrichment: (1) perfusion system with individual chambers and (2) hydroponic growth from tiller cuttings.
25896375	1	31	theme	%	147:147	arg1	O					153:153	50 % D 2 O	144:153	50 % D 2 O	144:153	The bioenergy crop switchgrass was grown hydroponically from tiller cuttings in 50 % D 2 O to obtain biomass with 34 % deuterium substitution and physicochemical properties similar to those of H 2 O-grown switchgrass controls.
25896375	2	32	theme	systems	485:491	arg1	transitions					462:472	molecular structural transitions	441:472	molecular structural transitions of complex systems such as plant cell walls	441:516	Deuterium enrichment of biological materials can potentially enable expanded experimental use of small angle neutron scattering (SANS) to investigate molecular structural transitions of complex systems such as plant cell walls.
25896375	3	33	theme	isotopic	648:655	arg1	enrichment					657:666	controlled isotopic enrichment	637:666	controlled isotopic enrichment	637:666	Two key advances have been made that facilitate cultivation of switchgrass, an important forage and biofuel crop, for controlled isotopic enrichment: (1) perfusion system with individual chambers and (2) hydroponic growth from tiller cuttings.
25896375	9	34	theme	deuterated	1385:1394	arg1	biomass					1412:1418	deuterated lignocellulosic biomass	1385:1418	deuterated lignocellulosic biomass	1385:1418	This capability to produce deuterated lignocellulosic biomass under controlled conditions will enhance investigation of cell wall structure and its deconstruction by neutron scattering and NMR techniques.
25896375	8	35	with	detection	1264:1272	arg1	FTIR					1315:1318	FTIR	1315:1318	FTIR	1315:1318	Deuterium incorporation was confirmed by detection of the O-D and C-D stretching peaks with FTIR and quantified by (1)H- and (2)H-NMR.
25896375	7	36	theme	hydroponic	1133:1142	arg1	cultivation					1144:1154	continuous hydroponic cultivation	1122:1154	continuous hydroponic cultivation using growth medium consisting of basal mineral salts in 50 % D2O	1122:1220	Using this improved method, gram quantities of switchgrass leaves and stems were produced by continuous hydroponic cultivation using growth medium consisting of basal mineral salts in 50 % D2O.
25896375	2	37	theme	materials	326:334	arg1	enrichment					301:310	Deuterium enrichment	291:310	Deuterium enrichment of biological materials	291:334	Deuterium enrichment of biological materials can potentially enable expanded experimental use of small angle neutron scattering (SANS) to investigate molecular structural transitions of complex systems such as plant cell walls.
25896375	1	38	theme	D	149:149	arg1	O					153:153	50 % D 2 O	144:153	50 % D 2 O	144:153	The bioenergy crop switchgrass was grown hydroponically from tiller cuttings in 50 % D 2 O to obtain biomass with 34 % deuterium substitution and physicochemical properties similar to those of H 2 O-grown switchgrass controls.
25896375	5	39	from	chambers	925:932	arg1	outflow					901:907	outflow	901:907	outflow from the growth chambers	901:932	Photosynthetic activity was monitored by measurement of CO2 in outflow from the growth chambers.
25896375	5	39	from	chambers	925:932	arg1	measurement					879:889	measurement	879:889	measurement of CO2 in outflow from the growth chambers	879:932	Photosynthetic activity was monitored by measurement of CO2 in outflow from the growth chambers.
25896375	2	40	theme	expanded	359:366	arg1	use					381:383	expanded experimental use	359:383	expanded experimental use of small angle neutron scattering (SANS) to investigate molecular structural transitions of complex systems such as plant cell walls	359:516	Deuterium enrichment of biological materials can potentially enable expanded experimental use of small angle neutron scattering (SANS) to investigate molecular structural transitions of complex systems such as plant cell walls.
25896375	3	41	theme	perfusion	673:681	arg1	system					683:688	perfusion system	673:688	(1) perfusion system with individual chambers	669:713	Two key advances have been made that facilitate cultivation of switchgrass, an important forage and biofuel crop, for controlled isotopic enrichment: (1) perfusion system with individual chambers and (2) hydroponic growth from tiller cuttings.
25896375	7	42	theme	stems	1099:1103	arg1	quantities					1062:1071	gram quantities	1057:1071	gram quantities of switchgrass leaves and stems	1057:1103	Using this improved method, gram quantities of switchgrass leaves and stems were produced by continuous hydroponic cultivation using growth medium consisting of basal mineral salts in 50 % D2O.
25896375	5	43	theme	Photosynthetic	838:851	arg1	activity					853:860	Photosynthetic activity	838:860	Photosynthetic activity	838:860	Photosynthetic activity was monitored by measurement of CO2 in outflow from the growth chambers.
25896375	8	44	theme	Deuterium	1223:1231	arg1	incorporation					1233:1245	Deuterium incorporation	1223:1245	Deuterium incorporation	1223:1245	Deuterium incorporation was confirmed by detection of the O-D and C-D stretching peaks with FTIR and quantified by (1)H- and (2)H-NMR.
25896375	8	45	dep	H-	1341:1342	arg1	1					1339:1339	1	1339:1339	1	1339:1339	Deuterium incorporation was confirmed by detection of the O-D and C-D stretching peaks with FTIR and quantified by (1)H- and (2)H-NMR.
25896375	7	46	theme	gram	1057:1060	arg1	quantities					1062:1071	gram quantities	1057:1071	gram quantities of switchgrass leaves and stems	1057:1103	Using this improved method, gram quantities of switchgrass leaves and stems were produced by continuous hydroponic cultivation using growth medium consisting of basal mineral salts in 50 % D2O.
25896375	2	47	theme	Deuterium	291:299	arg1	enrichment					301:310	Deuterium enrichment	291:310	Deuterium enrichment of biological materials	291:334	Deuterium enrichment of biological materials can potentially enable expanded experimental use of small angle neutron scattering (SANS) to investigate molecular structural transitions of complex systems such as plant cell walls.
25896375	9	48	theme	controlled	1426:1435	arg1	conditions					1437:1446	controlled conditions	1426:1446	controlled conditions	1426:1446	This capability to produce deuterated lignocellulosic biomass under controlled conditions will enhance investigation of cell wall structure and its deconstruction by neutron scattering and NMR techniques.
25896375	1	49	theme	deuterium	183:191	arg1	substitution					193:204	34 % deuterium substitution	178:204	34 % deuterium substitution	178:204	The bioenergy crop switchgrass was grown hydroponically from tiller cuttings in 50 % D 2 O to obtain biomass with 34 % deuterium substitution and physicochemical properties similar to those of H 2 O-grown switchgrass controls.
25896375	6	50	theme	Plant	935:939	arg1	morphology					941:950	Plant morphology	935:950	Plant morphology	935:950	Plant morphology and composition appeared normal compared to matched controls grown with H2O.
25896375	7	51	from	salts	1204:1208	arg1	D2O					1218:1220	50 % D2O	1213:1220	50 % D2O	1213:1220	Using this improved method, gram quantities of switchgrass leaves and stems were produced by continuous hydroponic cultivation using growth medium consisting of basal mineral salts in 50 % D2O.
25896375	3	52	theme	switchgrass	582:592	arg1	cultivation					567:577	cultivation	567:577	cultivation of switchgrass, an important forage	567:613	Two key advances have been made that facilitate cultivation of switchgrass, an important forage and biofuel crop, for controlled isotopic enrichment: (1) perfusion system with individual chambers and (2) hydroponic growth from tiller cuttings.
25896375	1	53	theme	tiller	125:130	arg1	cuttings					132:139	tiller cuttings	125:139	tiller cuttings in 50 % D 2 O	125:153	The bioenergy crop switchgrass was grown hydroponically from tiller cuttings in 50 % D 2 O to obtain biomass with 34 % deuterium substitution and physicochemical properties similar to those of H 2 O-grown switchgrass controls.
25896375	8	54	theme	O-D	1281:1283	arg1	detection					1264:1272	detection	1264:1272	detection of the O-D and C-D stretching peaks with FTIR	1264:1318	Deuterium incorporation was confirmed by detection of the O-D and C-D stretching peaks with FTIR and quantified by (1)H- and (2)H-NMR.
25896375	3	55	theme	tiller	746:751	arg1	cuttings					753:760	tiller cuttings	746:760	tiller cuttings	746:760	Two key advances have been made that facilitate cultivation of switchgrass, an important forage and biofuel crop, for controlled isotopic enrichment: (1) perfusion system with individual chambers and (2) hydroponic growth from tiller cuttings.
25896375	9	56	theme	cell	1478:1481	arg1	structure					1488:1496	cell wall structure	1478:1496	cell wall structure	1478:1496	This capability to produce deuterated lignocellulosic biomass under controlled conditions will enhance investigation of cell wall structure and its deconstruction by neutron scattering and NMR techniques.
25896375	8	57	theme	C-D	1289:1291	arg1	peaks					1304:1308	the O-D and C-D stretching peaks	1277:1308	peaks	1304:1308	Deuterium incorporation was confirmed by detection of the O-D and C-D stretching peaks with FTIR and quantified by (1)H- and (2)H-NMR.
25896375	1	58	theme	34	178:179	arg1	%					181:181	%	181:181	%	181:181	The bioenergy crop switchgrass was grown hydroponically from tiller cuttings in 50 % D 2 O to obtain biomass with 34 % deuterium substitution and physicochemical properties similar to those of H 2 O-grown switchgrass controls.
25896375	9	59	theme	structure	1488:1496	arg1	investigation					1461:1473	investigation	1461:1473	investigation of cell wall structure	1461:1496	This capability to produce deuterated lignocellulosic biomass under controlled conditions will enhance investigation of cell wall structure and its deconstruction by neutron scattering and NMR techniques.
25896375	9	59	theme	structure	1488:1496	arg1	deconstruction					1506:1519	its deconstruction	1502:1519	its deconstruction by neutron scattering and NMR techniques	1502:1560	This capability to produce deuterated lignocellulosic biomass under controlled conditions will enhance investigation of cell wall structure and its deconstruction by neutron scattering and NMR techniques.
25896375	2	60	theme	plant	501:505	arg1	walls					512:516	plant cell walls	501:516	plant cell walls	501:516	Deuterium enrichment of biological materials can potentially enable expanded experimental use of small angle neutron scattering (SANS) to investigate molecular structural transitions of complex systems such as plant cell walls.
25896375	8	61	theme	peaks	1304:1308	arg1	detection					1264:1272	detection	1264:1272	detection of the O-D and C-D stretching peaks with FTIR	1264:1318	Deuterium incorporation was confirmed by detection of the O-D and C-D stretching peaks with FTIR and quantified by (1)H- and (2)H-NMR.
25896375	7	62	theme	50	1213:1214	arg1	%					1216:1216	%	1216:1216	%	1216:1216	Using this improved method, gram quantities of switchgrass leaves and stems were produced by continuous hydroponic cultivation using growth medium consisting of basal mineral salts in 50 % D2O.
25896375	6	63	theme	matched	996:1002	arg1	controls					1004:1011	matched controls	996:1011	matched controls grown with H2O	996:1026	Plant morphology and composition appeared normal compared to matched controls grown with H2O.
25896375	3	64	dep	growth	734:739	arg1	2					720:720	2	720:720	2	720:720	Two key advances have been made that facilitate cultivation of switchgrass, an important forage and biofuel crop, for controlled isotopic enrichment: (1) perfusion system with individual chambers and (2) hydroponic growth from tiller cuttings.
25896375	1	65	theme	bioenergy	68:76	arg1	switchgrass					83:93	The bioenergy crop switchgrass	64:93	The bioenergy crop switchgrass	64:93	The bioenergy crop switchgrass was grown hydroponically from tiller cuttings in 50 % D 2 O to obtain biomass with 34 % deuterium substitution and physicochemical properties similar to those of H 2 O-grown switchgrass controls.
25896375	7	66	theme	basal	1190:1194	arg1	salts					1204:1208	basal mineral salts	1190:1208	basal mineral salts in 50 % D2O	1190:1220	Using this improved method, gram quantities of switchgrass leaves and stems were produced by continuous hydroponic cultivation using growth medium consisting of basal mineral salts in 50 % D2O.
25896375	5	67	from	measurement	879:889	arg1	outflow					901:907	outflow	901:907	outflow from the growth chambers	901:932	Photosynthetic activity was monitored by measurement of CO2 in outflow from the growth chambers.
25896375	5	67	from	measurement	879:889	arg1	chambers					925:932	the growth chambers	914:932	the growth chambers	914:932	Photosynthetic activity was monitored by measurement of CO2 in outflow from the growth chambers.
25896375	1	68	theme	crop	78:81	arg1	switchgrass					83:93	The bioenergy crop switchgrass	64:93	The bioenergy crop switchgrass	64:93	The bioenergy crop switchgrass was grown hydroponically from tiller cuttings in 50 % D 2 O to obtain biomass with 34 % deuterium substitution and physicochemical properties similar to those of H 2 O-grown switchgrass controls.
25896375	2	69	theme	angle	394:398	arg1	SANS					420:423	SANS	420:423	SANS	420:423	Deuterium enrichment of biological materials can potentially enable expanded experimental use of small angle neutron scattering (SANS) to investigate molecular structural transitions of complex systems such as plant cell walls.
25896375	2	69	theme	angle	394:398	arg1	scattering					408:417	small angle neutron scattering	388:417	small angle neutron scattering (SANS)	388:424	Deuterium enrichment of biological materials can potentially enable expanded experimental use of small angle neutron scattering (SANS) to investigate molecular structural transitions of complex systems such as plant cell walls.
25896375	5	70	theme	growth	918:923	arg1	chambers					925:932	the growth chambers	914:932	the growth chambers	914:932	Photosynthetic activity was monitored by measurement of CO2 in outflow from the growth chambers.
25896375	2	71	theme	molecular	441:449	arg1	transitions					462:472	molecular structural transitions	441:472	molecular structural transitions of complex systems such as plant cell walls	441:516	Deuterium enrichment of biological materials can potentially enable expanded experimental use of small angle neutron scattering (SANS) to investigate molecular structural transitions of complex systems such as plant cell walls.
25896375	0	72	theme	switchgrass	25:35	arg1	Production					0:9	Production	0:9	Production of deuterated switchgrass by hydroponic cultivation	0:61	Production of deuterated switchgrass by hydroponic cultivation.
25896375	1	73	theme	O-grown	261:267	arg1	controls					281:288	H 2 O-grown switchgrass controls	257:288	H 2 O-grown switchgrass controls	257:288	The bioenergy crop switchgrass was grown hydroponically from tiller cuttings in 50 % D 2 O to obtain biomass with 34 % deuterium substitution and physicochemical properties similar to those of H 2 O-grown switchgrass controls.
25896375	3	74	with	growth	734:739	arg1	chambers					706:713	individual chambers	695:713	individual chambers	695:713	Two key advances have been made that facilitate cultivation of switchgrass, an important forage and biofuel crop, for controlled isotopic enrichment: (1) perfusion system with individual chambers and (2) hydroponic growth from tiller cuttings.
25896375	3	75	theme	controlled	637:646	arg1	enrichment					657:666	controlled isotopic enrichment	637:666	controlled isotopic enrichment	637:666	Two key advances have been made that facilitate cultivation of switchgrass, an important forage and biofuel crop, for controlled isotopic enrichment: (1) perfusion system with individual chambers and (2) hydroponic growth from tiller cuttings.
25896375	0	76	theme	hydroponic	40:49	arg1	cultivation					51:61	hydroponic cultivation	40:61	hydroponic cultivation	40:61	Production of deuterated switchgrass by hydroponic cultivation.
25896375	2	77	theme	complex	477:483	arg1	walls					512:516	plant cell walls	501:516	plant cell walls	501:516	Deuterium enrichment of biological materials can potentially enable expanded experimental use of small angle neutron scattering (SANS) to investigate molecular structural transitions of complex systems such as plant cell walls.
25896375	2	77	theme	complex	477:483	arg1	systems					485:491	complex systems	477:491	complex systems such as plant cell walls	477:516	Deuterium enrichment of biological materials can potentially enable expanded experimental use of small angle neutron scattering (SANS) to investigate molecular structural transitions of complex systems such as plant cell walls.
25896375	2	78	theme	scattering	408:417	arg1	use					381:383	expanded experimental use	359:383	expanded experimental use of small angle neutron scattering (SANS) to investigate molecular structural transitions of complex systems such as plant cell walls	359:516	Deuterium enrichment of biological materials can potentially enable expanded experimental use of small angle neutron scattering (SANS) to investigate molecular structural transitions of complex systems such as plant cell walls.
25896375	7	79	theme	continuous	1122:1131	arg1	cultivation					1144:1154	continuous hydroponic cultivation	1122:1154	continuous hydroponic cultivation using growth medium consisting of basal mineral salts in 50 % D2O	1122:1220	Using this improved method, gram quantities of switchgrass leaves and stems were produced by continuous hydroponic cultivation using growth medium consisting of basal mineral salts in 50 % D2O.
25896375	2	80	theme	experimental	368:379	arg1	use					381:383	expanded experimental use	359:383	expanded experimental use of small angle neutron scattering (SANS) to investigate molecular structural transitions of complex systems such as plant cell walls	359:516	Deuterium enrichment of biological materials can potentially enable expanded experimental use of small angle neutron scattering (SANS) to investigate molecular structural transitions of complex systems such as plant cell walls.
25896375	4	81	theme	several	800:806	arg1	months					808:813	several months	800:813	several months with periodic harvest	800:835	Plants were grown and maintained for several months with periodic harvest.
25896375	2	82	theme	biological	315:324	arg1	materials					326:334	biological materials	315:334	biological materials	315:334	Deuterium enrichment of biological materials can potentially enable expanded experimental use of small angle neutron scattering (SANS) to investigate molecular structural transitions of complex systems such as plant cell walls.
25896375	1	83	theme	physicochemical	210:224	arg1	properties					226:235	physicochemical properties	210:235	physicochemical properties similar to those of H 2 O-grown switchgrass controls	210:288	The bioenergy crop switchgrass was grown hydroponically from tiller cuttings in 50 % D 2 O to obtain biomass with 34 % deuterium substitution and physicochemical properties similar to those of H 2 O-grown switchgrass controls.
25896375	3	84	theme	key	523:525	arg1	advances					527:534	Two key advances	519:534	Two key advances	519:534	Two key advances have been made that facilitate cultivation of switchgrass, an important forage and biofuel crop, for controlled isotopic enrichment: (1) perfusion system with individual chambers and (2) hydroponic growth from tiller cuttings.
25896375	1	85	theme	similar	237:243	arg1	properties					226:235	physicochemical properties	210:235	physicochemical properties similar to those of H 2 O-grown switchgrass controls	210:288	The bioenergy crop switchgrass was grown hydroponically from tiller cuttings in 50 % D 2 O to obtain biomass with 34 % deuterium substitution and physicochemical properties similar to those of H 2 O-grown switchgrass controls.
25458767	3	0	theme	kg	650:651	arg1	-1					662:663	-1	662:663	-1	662:663	Results obtained indicated that anaerobic digestion by itself promoted the high biological stability of biomasses with a Potential Dynamic Respiration Index (PDRI) close to 1000 mgO2 kg V S(-1)h(-1).
25458767	3	0	theme	kg	650:651	arg1	h					660:660	1000 mgO2 kg V S(-1)h	640:660	1000 mgO2 kg V S(-1)h(-1)	640:664	Results obtained indicated that anaerobic digestion by itself promoted the high biological stability of biomasses with a Potential Dynamic Respiration Index (PDRI) close to 1000 mgO2 kg V S(-1)h(-1).
25458767	4	1	theme	different	757:765	arg1	results					767:773	remarkably different results	746:773	remarkably different results	746:773	Subsequent composting of digestates, with an added bulking agent, did not give remarkably different results, and led only to a slight modification of the characteristics of the initial non-composted mixtures; the composts obtained fully respected the legal limits for high quality compost.
25458767	5	2	theme	low	1185:1187	arg1	alkyl-C					1189:1195	a low alkyl-C	1183:1195	a low alkyl-C (12.42%) (i.e. volatile fatty acids, steroid-like molecules, aliphatic biopolymers and proteins)	1183:1292	Chemical studies of organic matter composition of the biomasses by using CP MAS (13)C NMR, indicated that the compost was composed of a high relative content of O-alkyl-C (71.47% of total C) (cellulose and hemicelluloses) and a low alkyl-C (12.42%) (i.e. volatile fatty acids, steroid-like molecules, aliphatic biopolymers and proteins).
25458767	5	2	theme	low	1185:1187	arg1	%					1203:1203	12.42%	1198:1203	12.42%	1198:1203	Chemical studies of organic matter composition of the biomasses by using CP MAS (13)C NMR, indicated that the compost was composed of a high relative content of O-alkyl-C (71.47% of total C) (cellulose and hemicelluloses) and a low alkyl-C (12.42%) (i.e. volatile fatty acids, steroid-like molecules, aliphatic biopolymers and proteins).
25458767	1	3	theme	paper	132:136	arg1	aim					120:122	The aim	116:122	The aim of this paper	116:136	The aim of this paper was to assess the characteristics of the solid fractions (SF) obtained by mechanical separation of digestate, their compostability and compost quality.
25458767	5	4	theme	relative	1098:1105	arg1	content					1107:1113	a high relative content	1091:1113	a high relative content of O-alkyl-C (71.47% of total C) (cellulose and hemicelluloses) and a low alkyl-C (12.42%) (i.e. volatile fatty acids, steroid-like molecules, aliphatic biopolymers and proteins)	1091:1292	Chemical studies of organic matter composition of the biomasses by using CP MAS (13)C NMR, indicated that the compost was composed of a high relative content of O-alkyl-C (71.47% of total C) (cellulose and hemicelluloses) and a low alkyl-C (12.42%) (i.e. volatile fatty acids, steroid-like molecules, aliphatic biopolymers and proteins).
25458767	4	5	theme	mixtures	866:873	arg1	characteristics					821:835	the characteristics	817:835	the characteristics of the initial non-composted mixtures	817:873	Subsequent composting of digestates, with an added bulking agent, did not give remarkably different results, and led only to a slight modification of the characteristics of the initial non-composted mixtures; the composts obtained fully respected the legal limits for high quality compost.
25458767	5	6	theme	fatty	1221:1225	arg1	acids					1227:1231	volatile fatty acids	1212:1231	volatile fatty acids	1212:1231	Chemical studies of organic matter composition of the biomasses by using CP MAS (13)C NMR, indicated that the compost was composed of a high relative content of O-alkyl-C (71.47% of total C) (cellulose and hemicelluloses) and a low alkyl-C (12.42%) (i.e. volatile fatty acids, steroid-like molecules, aliphatic biopolymers and proteins).
25458767	3	7	theme	Dynamic	598:604	arg1	PDRI					625:628	PDRI	625:628	PDRI	625:628	Results obtained indicated that anaerobic digestion by itself promoted the high biological stability of biomasses with a Potential Dynamic Respiration Index (PDRI) close to 1000 mgO2 kg V S(-1)h(-1).
25458767	3	7	theme	Dynamic	598:604	arg1	Index					618:622	a Potential Dynamic Respiration Index	586:622	a Potential Dynamic Respiration Index (PDRI) close to 1000 mgO2 kg V S(-1)h(-1)	586:664	Results obtained indicated that anaerobic digestion by itself promoted the high biological stability of biomasses with a Potential Dynamic Respiration Index (PDRI) close to 1000 mgO2 kg V S(-1)h(-1).
25458767	1	8	theme	mechanical	212:221	arg1	separation					223:232	mechanical separation	212:232	mechanical separation of digestate, their compostability and compost quality	212:287	The aim of this paper was to assess the characteristics of the solid fractions (SF) obtained by mechanical separation of digestate, their compostability and compost quality.
25458767	4	9	theme	characteristics	821:835	arg1	modification					801:812	a slight modification	792:812	a slight modification of the characteristics of the initial non-composted mixtures	792:873	Subsequent composting of digestates, with an added bulking agent, did not give remarkably different results, and led only to a slight modification of the characteristics of the initial non-composted mixtures; the composts obtained fully respected the legal limits for high quality compost.
25458767	3	10	theme	1000	640:643	arg1	mgO2					645:648	mgO2	645:648	mgO2	645:648	Results obtained indicated that anaerobic digestion by itself promoted the high biological stability of biomasses with a Potential Dynamic Respiration Index (PDRI) close to 1000 mgO2 kg V S(-1)h(-1).
25458767	4	11	theme	quality	940:946	arg1	compost					948:954	high quality compost	935:954	high quality compost	935:954	Subsequent composting of digestates, with an added bulking agent, did not give remarkably different results, and led only to a slight modification of the characteristics of the initial non-composted mixtures; the composts obtained fully respected the legal limits for high quality compost.
25458767	0	12	theme	Biological	71:80	arg1	processes					82:90	Biological processes	71:90	Biological processes	71:90	Composting of the solid fraction of digestate derived from pig slurry: Biological processes and compost properties.
25458767	5	13	theme	organic	977:983	arg1	composition					992:1002	organic matter composition	977:1002	organic matter composition of the biomasses	977:1019	Chemical studies of organic matter composition of the biomasses by using CP MAS (13)C NMR, indicated that the compost was composed of a high relative content of O-alkyl-C (71.47% of total C) (cellulose and hemicelluloses) and a low alkyl-C (12.42%) (i.e. volatile fatty acids, steroid-like molecules, aliphatic biopolymers and proteins).
25458767	5	14	dep	O-alkyl-C	1118:1126	arg1	C					1145:1145	total C	1139:1145	total C	1139:1145	Chemical studies of organic matter composition of the biomasses by using CP MAS (13)C NMR, indicated that the compost was composed of a high relative content of O-alkyl-C (71.47% of total C) (cellulose and hemicelluloses) and a low alkyl-C (12.42%) (i.e. volatile fatty acids, steroid-like molecules, aliphatic biopolymers and proteins).
25458767	5	14	dep	O-alkyl-C	1118:1126	arg1	cellulose					1149:1157	cellulose	1149:1157	cellulose	1149:1157	Chemical studies of organic matter composition of the biomasses by using CP MAS (13)C NMR, indicated that the compost was composed of a high relative content of O-alkyl-C (71.47% of total C) (cellulose and hemicelluloses) and a low alkyl-C (12.42%) (i.e. volatile fatty acids, steroid-like molecules, aliphatic biopolymers and proteins).
25458767	5	14	dep	O-alkyl-C	1118:1126	arg1	hemicelluloses					1163:1176	hemicelluloses	1163:1176	hemicelluloses	1163:1176	Chemical studies of organic matter composition of the biomasses by using CP MAS (13)C NMR, indicated that the compost was composed of a high relative content of O-alkyl-C (71.47% of total C) (cellulose and hemicelluloses) and a low alkyl-C (12.42%) (i.e. volatile fatty acids, steroid-like molecules, aliphatic biopolymers and proteins).
25458767	5	14	dep	O-alkyl-C	1118:1126	arg1	%					1134:1134	71.47%	1129:1134	71.47% of total C	1129:1145	Chemical studies of organic matter composition of the biomasses by using CP MAS (13)C NMR, indicated that the compost was composed of a high relative content of O-alkyl-C (71.47% of total C) (cellulose and hemicelluloses) and a low alkyl-C (12.42%) (i.e. volatile fatty acids, steroid-like molecules, aliphatic biopolymers and proteins).
25458767	3	15	theme	anaerobic	499:507	arg1	digestion					509:517	anaerobic digestion	499:517	anaerobic digestion by itself	499:527	Results obtained indicated that anaerobic digestion by itself promoted the high biological stability of biomasses with a Potential Dynamic Respiration Index (PDRI) close to 1000 mgO2 kg V S(-1)h(-1).
25458767	0	16	theme	compost	96:102	arg1	properties					104:113	compost properties	96:113	compost properties	96:113	Composting of the solid fraction of digestate derived from pig slurry: Biological processes and compost properties.
25458767	5	17	theme	matter	985:990	arg1	composition					992:1002	organic matter composition	977:1002	organic matter composition of the biomasses	977:1019	Chemical studies of organic matter composition of the biomasses by using CP MAS (13)C NMR, indicated that the compost was composed of a high relative content of O-alkyl-C (71.47% of total C) (cellulose and hemicelluloses) and a low alkyl-C (12.42%) (i.e. volatile fatty acids, steroid-like molecules, aliphatic biopolymers and proteins).
25458767	5	18	theme	steroid-like	1234:1245	arg1	molecules					1247:1255	steroid-like molecules	1234:1255	steroid-like molecules	1234:1255	Chemical studies of organic matter composition of the biomasses by using CP MAS (13)C NMR, indicated that the compost was composed of a high relative content of O-alkyl-C (71.47% of total C) (cellulose and hemicelluloses) and a low alkyl-C (12.42%) (i.e. volatile fatty acids, steroid-like molecules, aliphatic biopolymers and proteins).
25458767	2	19	theme	Northern	451:458	arg1	Italy					460:464	Northern Italy	451:464	Northern Italy	451:464	To do so, the SF of digestates obtained from anaerobic digestion of pig slurry, energy crops and agro-industrial residues were sampled in five plants located in Northern Italy.
25458767	3	20	theme	V	653:653	arg1	-1					662:663	-1	662:663	-1	662:663	Results obtained indicated that anaerobic digestion by itself promoted the high biological stability of biomasses with a Potential Dynamic Respiration Index (PDRI) close to 1000 mgO2 kg V S(-1)h(-1).
25458767	3	20	theme	V	653:653	arg1	h					660:660	1000 mgO2 kg V S(-1)h	640:660	1000 mgO2 kg V S(-1)h(-1)	640:664	Results obtained indicated that anaerobic digestion by itself promoted the high biological stability of biomasses with a Potential Dynamic Respiration Index (PDRI) close to 1000 mgO2 kg V S(-1)h(-1).
25458767	4	21	theme	Subsequent	667:676	arg1	composting					678:687	Subsequent composting	667:687	Subsequent composting	667:687	Subsequent composting of digestates, with an added bulking agent, did not give remarkably different results, and led only to a slight modification of the characteristics of the initial non-composted mixtures; the composts obtained fully respected the legal limits for high quality compost.
25458767	5	22	dep	alkyl-C	1189:1195	arg1	proteins					1284:1291	proteins	1284:1291	proteins	1284:1291	Chemical studies of organic matter composition of the biomasses by using CP MAS (13)C NMR, indicated that the compost was composed of a high relative content of O-alkyl-C (71.47% of total C) (cellulose and hemicelluloses) and a low alkyl-C (12.42%) (i.e. volatile fatty acids, steroid-like molecules, aliphatic biopolymers and proteins).
25458767	5	22	dep	alkyl-C	1189:1195	arg1	molecules					1247:1255	steroid-like molecules	1234:1255	steroid-like molecules	1234:1255	Chemical studies of organic matter composition of the biomasses by using CP MAS (13)C NMR, indicated that the compost was composed of a high relative content of O-alkyl-C (71.47% of total C) (cellulose and hemicelluloses) and a low alkyl-C (12.42%) (i.e. volatile fatty acids, steroid-like molecules, aliphatic biopolymers and proteins).
25458767	5	22	dep	alkyl-C	1189:1195	arg1	biopolymers					1268:1278	aliphatic biopolymers	1258:1278	aliphatic biopolymers	1258:1278	Chemical studies of organic matter composition of the biomasses by using CP MAS (13)C NMR, indicated that the compost was composed of a high relative content of O-alkyl-C (71.47% of total C) (cellulose and hemicelluloses) and a low alkyl-C (12.42%) (i.e. volatile fatty acids, steroid-like molecules, aliphatic biopolymers and proteins).
25458767	5	22	dep	alkyl-C	1189:1195	arg1	acids					1227:1231	volatile fatty acids	1212:1231	volatile fatty acids	1212:1231	Chemical studies of organic matter composition of the biomasses by using CP MAS (13)C NMR, indicated that the compost was composed of a high relative content of O-alkyl-C (71.47% of total C) (cellulose and hemicelluloses) and a low alkyl-C (12.42%) (i.e. volatile fatty acids, steroid-like molecules, aliphatic biopolymers and proteins).
25458767	2	23	theme	anaerobic	335:343	arg1	digestion					345:353	anaerobic digestion	335:353	anaerobic digestion of pig slurry, energy crops and agro-industrial residues	335:410	To do so, the SF of digestates obtained from anaerobic digestion of pig slurry, energy crops and agro-industrial residues were sampled in five plants located in Northern Italy.
25458767	2	24	theme	agro-industrial	387:401	arg1	residues					403:410	agro-industrial residues	387:410	agro-industrial residues	387:410	To do so, the SF of digestates obtained from anaerobic digestion of pig slurry, energy crops and agro-industrial residues were sampled in five plants located in Northern Italy.
25458767	3	25	theme	S	655:655	arg1	-1					662:663	-1	662:663	-1	662:663	Results obtained indicated that anaerobic digestion by itself promoted the high biological stability of biomasses with a Potential Dynamic Respiration Index (PDRI) close to 1000 mgO2 kg V S(-1)h(-1).
25458767	3	25	theme	S	655:655	arg1	h					660:660	1000 mgO2 kg V S(-1)h	640:660	1000 mgO2 kg V S(-1)h(-1)	640:664	Results obtained indicated that anaerobic digestion by itself promoted the high biological stability of biomasses with a Potential Dynamic Respiration Index (PDRI) close to 1000 mgO2 kg V S(-1)h(-1).
25458767	5	26	theme	aliphatic	1258:1266	arg1	biopolymers					1268:1278	aliphatic biopolymers	1258:1278	aliphatic biopolymers	1258:1278	Chemical studies of organic matter composition of the biomasses by using CP MAS (13)C NMR, indicated that the compost was composed of a high relative content of O-alkyl-C (71.47% of total C) (cellulose and hemicelluloses) and a low alkyl-C (12.42%) (i.e. volatile fatty acids, steroid-like molecules, aliphatic biopolymers and proteins).
25458767	4	27	theme	non-composted	852:864	arg1	mixtures					866:873	the initial non-composted mixtures	840:873	the initial non-composted mixtures	840:873	Subsequent composting of digestates, with an added bulking agent, did not give remarkably different results, and led only to a slight modification of the characteristics of the initial non-composted mixtures; the composts obtained fully respected the legal limits for high quality compost.
25458767	1	28	theme	digestate	237:245	arg1	separation					223:232	mechanical separation	212:232	mechanical separation of digestate, their compostability and compost quality	212:287	The aim of this paper was to assess the characteristics of the solid fractions (SF) obtained by mechanical separation of digestate, their compostability and compost quality.
25458767	2	29	from	Italy	460:464	arg1	located					440:446	located	440:446	located	440:446	To do so, the SF of digestates obtained from anaerobic digestion of pig slurry, energy crops and agro-industrial residues were sampled in five plants located in Northern Italy.
25458767	5	30	theme	Chemical	957:964	arg1	studies					966:972	Chemical studies	957:972	Chemical studies of organic matter composition of the biomasses	957:1019	Chemical studies of organic matter composition of the biomasses by using CP MAS (13)C NMR, indicated that the compost was composed of a high relative content of O-alkyl-C (71.47% of total C) (cellulose and hemicelluloses) and a low alkyl-C (12.42%) (i.e. volatile fatty acids, steroid-like molecules, aliphatic biopolymers and proteins).
25458767	2	31	theme	crops	377:381	arg1	digestion					345:353	anaerobic digestion	335:353	anaerobic digestion of pig slurry, energy crops and agro-industrial residues	335:410	To do so, the SF of digestates obtained from anaerobic digestion of pig slurry, energy crops and agro-industrial residues were sampled in five plants located in Northern Italy.
25458767	5	32	theme	C	1041:1041	arg1	NMR					1043:1045	CP MAS (13)C NMR	1030:1045	CP MAS (13)C NMR	1030:1045	Chemical studies of organic matter composition of the biomasses by using CP MAS (13)C NMR, indicated that the compost was composed of a high relative content of O-alkyl-C (71.47% of total C) (cellulose and hemicelluloses) and a low alkyl-C (12.42%) (i.e. volatile fatty acids, steroid-like molecules, aliphatic biopolymers and proteins).
25458767	4	33	theme	slight	794:799	arg1	modification					801:812	a slight modification	792:812	a slight modification of the characteristics of the initial non-composted mixtures	792:873	Subsequent composting of digestates, with an added bulking agent, did not give remarkably different results, and led only to a slight modification of the characteristics of the initial non-composted mixtures; the composts obtained fully respected the legal limits for high quality compost.
25458767	0	34	attach	derived	46:52	arg1	slurry					63:68	pig slurry	59:68	pig slurry	59:68	Composting of the solid fraction of digestate derived from pig slurry: Biological processes and compost properties.
25458767	0	34	attach	derived	46:52	arg2	digestate					36:44	digestate	36:44	digestate derived from pig slurry	36:68	Composting of the solid fraction of digestate derived from pig slurry: Biological processes and compost properties.
25458767	4	35	theme	added	712:716	arg1	agent					726:730	an added bulking agent	709:730	an added bulking agent	709:730	Subsequent composting of digestates, with an added bulking agent, did not give remarkably different results, and led only to a slight modification of the characteristics of the initial non-composted mixtures; the composts obtained fully respected the legal limits for high quality compost.
25458767	2	36	theme	energy	370:375	arg1	crops					377:381	energy crops	370:381	energy crops	370:381	To do so, the SF of digestates obtained from anaerobic digestion of pig slurry, energy crops and agro-industrial residues were sampled in five plants located in Northern Italy.
25458767	5	37	theme	biomasses	1011:1019	arg1	composition					992:1002	organic matter composition	977:1002	organic matter composition of the biomasses	977:1019	Chemical studies of organic matter composition of the biomasses by using CP MAS (13)C NMR, indicated that the compost was composed of a high relative content of O-alkyl-C (71.47% of total C) (cellulose and hemicelluloses) and a low alkyl-C (12.42%) (i.e. volatile fatty acids, steroid-like molecules, aliphatic biopolymers and proteins).
25458767	3	38	theme	Respiration	606:616	arg1	PDRI					625:628	PDRI	625:628	PDRI	625:628	Results obtained indicated that anaerobic digestion by itself promoted the high biological stability of biomasses with a Potential Dynamic Respiration Index (PDRI) close to 1000 mgO2 kg V S(-1)h(-1).
25458767	3	38	theme	Respiration	606:616	arg1	Index					618:622	a Potential Dynamic Respiration Index	586:622	a Potential Dynamic Respiration Index (PDRI) close to 1000 mgO2 kg V S(-1)h(-1)	586:664	Results obtained indicated that anaerobic digestion by itself promoted the high biological stability of biomasses with a Potential Dynamic Respiration Index (PDRI) close to 1000 mgO2 kg V S(-1)h(-1).
25458767	4	39	with	composting	678:687	arg1	agent					726:730	an added bulking agent	709:730	an added bulking agent	709:730	Subsequent composting of digestates, with an added bulking agent, did not give remarkably different results, and led only to a slight modification of the characteristics of the initial non-composted mixtures; the composts obtained fully respected the legal limits for high quality compost.
25458767	5	40	theme	C	1145:1145	arg1	C					1145:1145	total C	1139:1145	total C	1139:1145	Chemical studies of organic matter composition of the biomasses by using CP MAS (13)C NMR, indicated that the compost was composed of a high relative content of O-alkyl-C (71.47% of total C) (cellulose and hemicelluloses) and a low alkyl-C (12.42%) (i.e. volatile fatty acids, steroid-like molecules, aliphatic biopolymers and proteins).
25458767	5	40	theme	C	1145:1145	arg1	%					1134:1134	71.47%	1129:1134	71.47% of total C	1129:1145	Chemical studies of organic matter composition of the biomasses by using CP MAS (13)C NMR, indicated that the compost was composed of a high relative content of O-alkyl-C (71.47% of total C) (cellulose and hemicelluloses) and a low alkyl-C (12.42%) (i.e. volatile fatty acids, steroid-like molecules, aliphatic biopolymers and proteins).
25458767	2	41	theme	slurry	362:367	arg1	digestion					345:353	anaerobic digestion	335:353	anaerobic digestion of pig slurry, energy crops and agro-industrial residues	335:410	To do so, the SF of digestates obtained from anaerobic digestion of pig slurry, energy crops and agro-industrial residues were sampled in five plants located in Northern Italy.
25458767	1	42	theme	solid	179:183	arg1	SF					196:197	SF	196:197	SF	196:197	The aim of this paper was to assess the characteristics of the solid fractions (SF) obtained by mechanical separation of digestate, their compostability and compost quality.
25458767	1	42	theme	solid	179:183	arg1	fractions					185:193	the solid fractions	175:193	the solid fractions (SF) obtained by mechanical separation of digestate, their compostability and compost quality	175:287	The aim of this paper was to assess the characteristics of the solid fractions (SF) obtained by mechanical separation of digestate, their compostability and compost quality.
25458767	3	43	theme	biological	547:556	arg1	stability					558:566	the high biological stability	538:566	the high biological stability of biomasses	538:579	Results obtained indicated that anaerobic digestion by itself promoted the high biological stability of biomasses with a Potential Dynamic Respiration Index (PDRI) close to 1000 mgO2 kg V S(-1)h(-1).
25458767	2	44	theme	pig	358:360	arg1	slurry					362:367	pig slurry	358:367	pig slurry	358:367	To do so, the SF of digestates obtained from anaerobic digestion of pig slurry, energy crops and agro-industrial residues were sampled in five plants located in Northern Italy.
25458767	0	45	theme	fraction	24:31	arg1	Composting					0:9	Composting	0:9	Composting of the solid fraction of digestate derived from pig slurry: Biological processes and compost properties.	0:114	Composting of the solid fraction of digestate derived from pig slurry: Biological processes and compost properties.
25458767	5	46	theme	CP	1030:1031	arg1	NMR					1043:1045	CP MAS (13)C NMR	1030:1045	CP MAS (13)C NMR	1030:1045	Chemical studies of organic matter composition of the biomasses by using CP MAS (13)C NMR, indicated that the compost was composed of a high relative content of O-alkyl-C (71.47% of total C) (cellulose and hemicelluloses) and a low alkyl-C (12.42%) (i.e. volatile fatty acids, steroid-like molecules, aliphatic biopolymers and proteins).
25458767	3	47	theme	Potential	588:596	arg1	PDRI					625:628	PDRI	625:628	PDRI	625:628	Results obtained indicated that anaerobic digestion by itself promoted the high biological stability of biomasses with a Potential Dynamic Respiration Index (PDRI) close to 1000 mgO2 kg V S(-1)h(-1).
25458767	3	47	theme	Potential	588:596	arg1	Index					618:622	a Potential Dynamic Respiration Index	586:622	a Potential Dynamic Respiration Index (PDRI) close to 1000 mgO2 kg V S(-1)h(-1)	586:664	Results obtained indicated that anaerobic digestion by itself promoted the high biological stability of biomasses with a Potential Dynamic Respiration Index (PDRI) close to 1000 mgO2 kg V S(-1)h(-1).
25458767	0	48	theme	solid	18:22	arg1	fraction					24:31	the solid fraction	14:31	the solid fraction of digestate derived from pig slurry	14:68	Composting of the solid fraction of digestate derived from pig slurry: Biological processes and compost properties.
25458767	5	49	theme	alkyl-C	1189:1195	arg1	content					1107:1113	a high relative content	1091:1113	a high relative content of O-alkyl-C (71.47% of total C) (cellulose and hemicelluloses) and a low alkyl-C (12.42%) (i.e. volatile fatty acids, steroid-like molecules, aliphatic biopolymers and proteins)	1091:1292	Chemical studies of organic matter composition of the biomasses by using CP MAS (13)C NMR, indicated that the compost was composed of a high relative content of O-alkyl-C (71.47% of total C) (cellulose and hemicelluloses) and a low alkyl-C (12.42%) (i.e. volatile fatty acids, steroid-like molecules, aliphatic biopolymers and proteins).
25458767	1	50	theme	fractions	185:193	arg1	characteristics					156:170	the characteristics	152:170	the characteristics of the solid fractions (SF) obtained by mechanical separation of digestate, their compostability and compost quality	152:287	The aim of this paper was to assess the characteristics of the solid fractions (SF) obtained by mechanical separation of digestate, their compostability and compost quality.
25458767	1	51	theme	compost	273:279	arg1	digestate					237:245	digestate	237:245	digestate	237:245	The aim of this paper was to assess the characteristics of the solid fractions (SF) obtained by mechanical separation of digestate, their compostability and compost quality.
25458767	1	51	theme	compost	273:279	arg1	quality					281:287	compost quality	273:287	compost quality	273:287	The aim of this paper was to assess the characteristics of the solid fractions (SF) obtained by mechanical separation of digestate, their compostability and compost quality.
25458767	4	52	theme	bulking	718:724	arg1	agent					726:730	an added bulking agent	709:730	an added bulking agent	709:730	Subsequent composting of digestates, with an added bulking agent, did not give remarkably different results, and led only to a slight modification of the characteristics of the initial non-composted mixtures; the composts obtained fully respected the legal limits for high quality compost.
25458767	2	53	from	located	440:446	arg1	Italy					460:464	Northern Italy	451:464	Northern Italy	451:464	To do so, the SF of digestates obtained from anaerobic digestion of pig slurry, energy crops and agro-industrial residues were sampled in five plants located in Northern Italy.
25458767	5	54	theme	composition	992:1002	arg1	studies					966:972	Chemical studies	957:972	Chemical studies of organic matter composition of the biomasses	957:1019	Chemical studies of organic matter composition of the biomasses by using CP MAS (13)C NMR, indicated that the compost was composed of a high relative content of O-alkyl-C (71.47% of total C) (cellulose and hemicelluloses) and a low alkyl-C (12.42%) (i.e. volatile fatty acids, steroid-like molecules, aliphatic biopolymers and proteins).
25458767	0	55	theme	digestate	36:44	arg1	fraction					24:31	the solid fraction	14:31	the solid fraction of digestate derived from pig slurry	14:68	Composting of the solid fraction of digestate derived from pig slurry: Biological processes and compost properties.
25458767	2	56	theme	residues	403:410	arg1	digestion					345:353	anaerobic digestion	335:353	anaerobic digestion of pig slurry, energy crops and agro-industrial residues	335:410	To do so, the SF of digestates obtained from anaerobic digestion of pig slurry, energy crops and agro-industrial residues were sampled in five plants located in Northern Italy.
25458767	3	57	theme	high	542:545	arg1	stability					558:566	the high biological stability	538:566	the high biological stability of biomasses	538:579	Results obtained indicated that anaerobic digestion by itself promoted the high biological stability of biomasses with a Potential Dynamic Respiration Index (PDRI) close to 1000 mgO2 kg V S(-1)h(-1).
25458767	5	58	theme	O-alkyl-C	1118:1126	arg1	content					1107:1113	a high relative content	1091:1113	a high relative content of O-alkyl-C (71.47% of total C) (cellulose and hemicelluloses) and a low alkyl-C (12.42%) (i.e. volatile fatty acids, steroid-like molecules, aliphatic biopolymers and proteins)	1091:1292	Chemical studies of organic matter composition of the biomasses by using CP MAS (13)C NMR, indicated that the compost was composed of a high relative content of O-alkyl-C (71.47% of total C) (cellulose and hemicelluloses) and a low alkyl-C (12.42%) (i.e. volatile fatty acids, steroid-like molecules, aliphatic biopolymers and proteins).
25458767	3	59	theme	biomasses	571:579	arg1	stability					558:566	the high biological stability	538:566	the high biological stability of biomasses	538:579	Results obtained indicated that anaerobic digestion by itself promoted the high biological stability of biomasses with a Potential Dynamic Respiration Index (PDRI) close to 1000 mgO2 kg V S(-1)h(-1).
25458767	4	60	theme	legal	918:922	arg1	limits					924:929	the legal limits	914:929	the legal limits for high quality compost	914:954	Subsequent composting of digestates, with an added bulking agent, did not give remarkably different results, and led only to a slight modification of the characteristics of the initial non-composted mixtures; the composts obtained fully respected the legal limits for high quality compost.
25458767	4	61	theme	digestates	692:701	arg1	composting					678:687	Subsequent composting	667:687	Subsequent composting	667:687	Subsequent composting of digestates, with an added bulking agent, did not give remarkably different results, and led only to a slight modification of the characteristics of the initial non-composted mixtures; the composts obtained fully respected the legal limits for high quality compost.
25458767	5	62	theme	total	1139:1143	arg1	C					1145:1145	total C	1139:1145	total C	1139:1145	Chemical studies of organic matter composition of the biomasses by using CP MAS (13)C NMR, indicated that the compost was composed of a high relative content of O-alkyl-C (71.47% of total C) (cellulose and hemicelluloses) and a low alkyl-C (12.42%) (i.e. volatile fatty acids, steroid-like molecules, aliphatic biopolymers and proteins).
25458767	0	63	theme	pig	59:61	arg1	slurry					63:68	pig slurry	59:68	pig slurry	59:68	Composting of the solid fraction of digestate derived from pig slurry: Biological processes and compost properties.
25458767	5	64	theme	volatile	1212:1219	arg1	acids					1227:1231	volatile fatty acids	1212:1231	volatile fatty acids	1212:1231	Chemical studies of organic matter composition of the biomasses by using CP MAS (13)C NMR, indicated that the compost was composed of a high relative content of O-alkyl-C (71.47% of total C) (cellulose and hemicelluloses) and a low alkyl-C (12.42%) (i.e. volatile fatty acids, steroid-like molecules, aliphatic biopolymers and proteins).
25458767	5	65	theme	high	1093:1096	arg1	content					1107:1113	a high relative content	1091:1113	a high relative content of O-alkyl-C (71.47% of total C) (cellulose and hemicelluloses) and a low alkyl-C (12.42%) (i.e. volatile fatty acids, steroid-like molecules, aliphatic biopolymers and proteins)	1091:1292	Chemical studies of organic matter composition of the biomasses by using CP MAS (13)C NMR, indicated that the compost was composed of a high relative content of O-alkyl-C (71.47% of total C) (cellulose and hemicelluloses) and a low alkyl-C (12.42%) (i.e. volatile fatty acids, steroid-like molecules, aliphatic biopolymers and proteins).
25458767	2	66	theme	digestates	310:319	arg1	SF					304:305	the SF	300:305	the SF of digestates obtained from anaerobic digestion of pig slurry, energy crops and agro-industrial residues	300:410	To do so, the SF of digestates obtained from anaerobic digestion of pig slurry, energy crops and agro-industrial residues were sampled in five plants located in Northern Italy.
25458767	5	67	dep	acids	1227:1231	arg1	i.e.					1207:1210	i.e.	1207:1210	i.e.	1207:1210	Chemical studies of organic matter composition of the biomasses by using CP MAS (13)C NMR, indicated that the compost was composed of a high relative content of O-alkyl-C (71.47% of total C) (cellulose and hemicelluloses) and a low alkyl-C (12.42%) (i.e. volatile fatty acids, steroid-like molecules, aliphatic biopolymers and proteins).
25458767	4	68	theme	initial	844:850	arg1	mixtures					866:873	the initial non-composted mixtures	840:873	the initial non-composted mixtures	840:873	Subsequent composting of digestates, with an added bulking agent, did not give remarkably different results, and led only to a slight modification of the characteristics of the initial non-composted mixtures; the composts obtained fully respected the legal limits for high quality compost.
25458767	2	69	theme	located	440:446	arg1	plants					433:438	five plants	428:438	five plants located in Northern Italy	428:464	To do so, the SF of digestates obtained from anaerobic digestion of pig slurry, energy crops and agro-industrial residues were sampled in five plants located in Northern Italy.
25458767	3	70	theme	mgO2	645:648	arg1	-1					662:663	-1	662:663	-1	662:663	Results obtained indicated that anaerobic digestion by itself promoted the high biological stability of biomasses with a Potential Dynamic Respiration Index (PDRI) close to 1000 mgO2 kg V S(-1)h(-1).
25458767	3	70	theme	mgO2	645:648	arg1	h					660:660	1000 mgO2 kg V S(-1)h	640:660	1000 mgO2 kg V S(-1)h(-1)	640:664	Results obtained indicated that anaerobic digestion by itself promoted the high biological stability of biomasses with a Potential Dynamic Respiration Index (PDRI) close to 1000 mgO2 kg V S(-1)h(-1).
25458767	0	71	dep	Composting	0:9	arg1	processes					82:90	Biological processes	71:90	Biological processes	71:90	Composting of the solid fraction of digestate derived from pig slurry: Biological processes and compost properties.
25458767	0	71	dep	Composting	0:9	arg1	properties					104:113	compost properties	96:113	compost properties	96:113	Composting of the solid fraction of digestate derived from pig slurry: Biological processes and compost properties.
25458767	4	72	theme	high	935:938	arg1	compost					948:954	high quality compost	935:954	high quality compost	935:954	Subsequent composting of digestates, with an added bulking agent, did not give remarkably different results, and led only to a slight modification of the characteristics of the initial non-composted mixtures; the composts obtained fully respected the legal limits for high quality compost.
25458767	5	73	theme	MAS	1033:1035	arg1	NMR					1043:1045	CP MAS (13)C NMR	1030:1045	CP MAS (13)C NMR	1030:1045	Chemical studies of organic matter composition of the biomasses by using CP MAS (13)C NMR, indicated that the compost was composed of a high relative content of O-alkyl-C (71.47% of total C) (cellulose and hemicelluloses) and a low alkyl-C (12.42%) (i.e. volatile fatty acids, steroid-like molecules, aliphatic biopolymers and proteins).
25977029	2	0	theme	native	416:421	arg1	form					423:426	its native form	412:426	its native form	412:426	Water-extracted pectin was considered closest to its native form.
25977029	5	1	theme	extraction	857:866	arg1	methods					868:874	enzymatic extraction methods	847:874	enzymatic extraction methods	847:874	The Mw and root mean square (RMS) radius varied with the extraction methods with values of 8.4 × 10(5) g/mol and 92 nm, 8.5 × 10(5)g/mol and 102 nm, 6.7 × 10(5) g/mol and 52 nm for acid, water and enzymatic extraction methods, respectively.
25977029	0	2	theme	Actinidia	70:78	arg1	kiwifruit					59:67	gold kiwifruit	54:67	gold kiwifruit (Actinidia chinensis)	54:89	Extraction and characterisation of pomace pectin from gold kiwifruit (Actinidia chinensis).
25977029	0	2	theme	Actinidia	70:78	arg1	chinensis					80:88	Actinidia chinensis	70:88	Actinidia chinensis	70:88	Extraction and characterisation of pomace pectin from gold kiwifruit (Actinidia chinensis).
25977029	1	3	theme	neutral	284:290	arg1	composition					298:308	neutral sugar composition	284:308	neutral sugar composition	284:308	Gold kiwifruit pomace extracted using citric acid, water and enzyme (Celluclast 1.5L) were studied in terms of pectin yield, protein, ash, non-starch polysaccharide, galacturonic acid (GalA), neutral sugar composition, molar mass (Mw), viscosity and degree of branching.
25977029	6	4	theme	enzyme-extracted	1024:1039	arg1	pectin					1041:1046	enzyme-extracted pectin	1024:1046	enzyme-extracted pectin	1024:1046	Similar trend was observed for pectin viscosity, with water-extracted pectin giving a slightly higher viscosity followed by acid and enzyme-extracted pectin.
25977029	1	5	theme	branching	352:360	arg1	acid					271:274	galacturonic acid	258:274	galacturonic acid (GalA)	258:281	Gold kiwifruit pomace extracted using citric acid, water and enzyme (Celluclast 1.5L) were studied in terms of pectin yield, protein, ash, non-starch polysaccharide, galacturonic acid (GalA), neutral sugar composition, molar mass (Mw), viscosity and degree of branching.
25977029	1	5	theme	branching	352:360	arg1	composition					298:308	neutral sugar composition	284:308	neutral sugar composition	284:308	Gold kiwifruit pomace extracted using citric acid, water and enzyme (Celluclast 1.5L) were studied in terms of pectin yield, protein, ash, non-starch polysaccharide, galacturonic acid (GalA), neutral sugar composition, molar mass (Mw), viscosity and degree of branching.
25977029	1	5	theme	branching	352:360	arg1	GalA					277:280	GalA	277:280	GalA	277:280	Gold kiwifruit pomace extracted using citric acid, water and enzyme (Celluclast 1.5L) were studied in terms of pectin yield, protein, ash, non-starch polysaccharide, galacturonic acid (GalA), neutral sugar composition, molar mass (Mw), viscosity and degree of branching.
25977029	1	5	theme	branching	352:360	arg1	yield					210:214	pectin yield	203:214	pectin yield	203:214	Gold kiwifruit pomace extracted using citric acid, water and enzyme (Celluclast 1.5L) were studied in terms of pectin yield, protein, ash, non-starch polysaccharide, galacturonic acid (GalA), neutral sugar composition, molar mass (Mw), viscosity and degree of branching.
25977029	1	5	theme	branching	352:360	arg1	viscosity					328:336	viscosity	328:336	viscosity	328:336	Gold kiwifruit pomace extracted using citric acid, water and enzyme (Celluclast 1.5L) were studied in terms of pectin yield, protein, ash, non-starch polysaccharide, galacturonic acid (GalA), neutral sugar composition, molar mass (Mw), viscosity and degree of branching.
25977029	1	5	theme	branching	352:360	arg1	mass					317:320	molar mass	311:320	molar mass (Mw)	311:325	Gold kiwifruit pomace extracted using citric acid, water and enzyme (Celluclast 1.5L) were studied in terms of pectin yield, protein, ash, non-starch polysaccharide, galacturonic acid (GalA), neutral sugar composition, molar mass (Mw), viscosity and degree of branching.
25977029	1	5	theme	branching	352:360	arg1	polysaccharide					242:255	non-starch polysaccharide	231:255	non-starch polysaccharide	231:255	Gold kiwifruit pomace extracted using citric acid, water and enzyme (Celluclast 1.5L) were studied in terms of pectin yield, protein, ash, non-starch polysaccharide, galacturonic acid (GalA), neutral sugar composition, molar mass (Mw), viscosity and degree of branching.
25977029	1	5	theme	branching	352:360	arg1	degree					342:347	degree	342:347	degree	342:347	Gold kiwifruit pomace extracted using citric acid, water and enzyme (Celluclast 1.5L) were studied in terms of pectin yield, protein, ash, non-starch polysaccharide, galacturonic acid (GalA), neutral sugar composition, molar mass (Mw), viscosity and degree of branching.
25977029	1	5	theme	branching	352:360	arg1	protein					217:223	protein	217:223	protein	217:223	Gold kiwifruit pomace extracted using citric acid, water and enzyme (Celluclast 1.5L) were studied in terms of pectin yield, protein, ash, non-starch polysaccharide, galacturonic acid (GalA), neutral sugar composition, molar mass (Mw), viscosity and degree of branching.
25977029	1	5	theme	branching	352:360	arg1	Mw					323:324	Mw	323:324	Mw	323:324	Gold kiwifruit pomace extracted using citric acid, water and enzyme (Celluclast 1.5L) were studied in terms of pectin yield, protein, ash, non-starch polysaccharide, galacturonic acid (GalA), neutral sugar composition, molar mass (Mw), viscosity and degree of branching.
25977029	1	5	theme	branching	352:360	arg1	ash					226:228	ash	226:228	ash	226:228	Gold kiwifruit pomace extracted using citric acid, water and enzyme (Celluclast 1.5L) were studied in terms of pectin yield, protein, ash, non-starch polysaccharide, galacturonic acid (GalA), neutral sugar composition, molar mass (Mw), viscosity and degree of branching.
25977029	3	6	theme	extracted	436:444	arg1	pectin					446:451	Enzyme extracted pectin	429:451	Enzyme extracted pectin	429:451	Enzyme extracted pectin showed the highest yield (∼ 4.5%w/w) as compared with the acid and water extraction methods (∼ 3.6-3.8%w/w).
25977029	1	7	theme	protein	217:223	arg1	terms					194:198	terms	194:198	terms of pectin yield, protein, ash, non-starch polysaccharide, galacturonic acid (GalA), neutral sugar composition, molar mass (Mw), viscosity and degree of branching	194:360	Gold kiwifruit pomace extracted using citric acid, water and enzyme (Celluclast 1.5L) were studied in terms of pectin yield, protein, ash, non-starch polysaccharide, galacturonic acid (GalA), neutral sugar composition, molar mass (Mw), viscosity and degree of branching.
25977029	1	8	theme	sugar	292:296	arg1	composition					298:308	neutral sugar composition	284:308	neutral sugar composition	284:308	Gold kiwifruit pomace extracted using citric acid, water and enzyme (Celluclast 1.5L) were studied in terms of pectin yield, protein, ash, non-starch polysaccharide, galacturonic acid (GalA), neutral sugar composition, molar mass (Mw), viscosity and degree of branching.
25977029	6	9	theme	pectin	922:927	arg1	viscosity					929:937	pectin viscosity	922:937	pectin viscosity	922:937	Similar trend was observed for pectin viscosity, with water-extracted pectin giving a slightly higher viscosity followed by acid and enzyme-extracted pectin.
25977029	5	10	theme	×	774:774	arg1	g/mol					781:785	8.5 × 10(5)g/mol	770:785	8.5 × 10(5)g/mol	770:785	The Mw and root mean square (RMS) radius varied with the extraction methods with values of 8.4 × 10(5) g/mol and 92 nm, 8.5 × 10(5)g/mol and 102 nm, 6.7 × 10(5) g/mol and 52 nm for acid, water and enzymatic extraction methods, respectively.
25977029	1	11	theme	composition	298:308	arg1	terms					194:198	terms	194:198	terms of pectin yield, protein, ash, non-starch polysaccharide, galacturonic acid (GalA), neutral sugar composition, molar mass (Mw), viscosity and degree of branching	194:360	Gold kiwifruit pomace extracted using citric acid, water and enzyme (Celluclast 1.5L) were studied in terms of pectin yield, protein, ash, non-starch polysaccharide, galacturonic acid (GalA), neutral sugar composition, molar mass (Mw), viscosity and degree of branching.
25977029	1	12	theme	ash	226:228	arg1	terms					194:198	terms	194:198	terms of pectin yield, protein, ash, non-starch polysaccharide, galacturonic acid (GalA), neutral sugar composition, molar mass (Mw), viscosity and degree of branching	194:360	Gold kiwifruit pomace extracted using citric acid, water and enzyme (Celluclast 1.5L) were studied in terms of pectin yield, protein, ash, non-starch polysaccharide, galacturonic acid (GalA), neutral sugar composition, molar mass (Mw), viscosity and degree of branching.
25977029	7	13	theme	kiwifruit	1077:1085	arg1	pectin					1094:1099	gold kiwifruit pomace pectin	1072:1099	gold kiwifruit pomace pectin	1072:1099	This study showed that gold kiwifruit pomace pectin has potential application in food products.
25977029	1	14	theme	molar	311:315	arg1	Mw					323:324	Mw	323:324	Mw	323:324	Gold kiwifruit pomace extracted using citric acid, water and enzyme (Celluclast 1.5L) were studied in terms of pectin yield, protein, ash, non-starch polysaccharide, galacturonic acid (GalA), neutral sugar composition, molar mass (Mw), viscosity and degree of branching.
25977029	1	14	theme	molar	311:315	arg1	mass					317:320	molar mass	311:320	molar mass (Mw)	311:325	Gold kiwifruit pomace extracted using citric acid, water and enzyme (Celluclast 1.5L) were studied in terms of pectin yield, protein, ash, non-starch polysaccharide, galacturonic acid (GalA), neutral sugar composition, molar mass (Mw), viscosity and degree of branching.
25977029	5	15	theme	root	661:664	arg1	radius					684:689	The Mw and root mean square (RMS) radius	650:689	radius	684:689	The Mw and root mean square (RMS) radius varied with the extraction methods with values of 8.4 × 10(5) g/mol and 92 nm, 8.5 × 10(5)g/mol and 102 nm, 6.7 × 10(5) g/mol and 52 nm for acid, water and enzymatic extraction methods, respectively.
25977029	3	16	theme	highest	464:470	arg1	yield					472:476	the highest yield	460:476	the highest yield (∼ 4.5%w/w)	460:488	Enzyme extracted pectin showed the highest yield (∼ 4.5%w/w) as compared with the acid and water extraction methods (∼ 3.6-3.8%w/w).
25977029	3	16	theme	highest	464:470	arg1	%					484:484	∼ 4.5%w/w	479:487	∼ 4.5%w/w	479:487	Enzyme extracted pectin showed the highest yield (∼ 4.5%w/w) as compared with the acid and water extraction methods (∼ 3.6-3.8%w/w).
25977029	7	17	theme	pomace	1087:1092	arg1	pectin					1094:1099	gold kiwifruit pomace pectin	1072:1099	gold kiwifruit pomace pectin	1072:1099	This study showed that gold kiwifruit pomace pectin has potential application in food products.
25977029	5	18	theme	RMS	679:681	arg1	radius					684:689	The Mw and root mean square (RMS) radius	650:689	radius	684:689	The Mw and root mean square (RMS) radius varied with the extraction methods with values of 8.4 × 10(5) g/mol and 92 nm, 8.5 × 10(5)g/mol and 102 nm, 6.7 × 10(5) g/mol and 52 nm for acid, water and enzymatic extraction methods, respectively.
25977029	1	19	theme	mass	317:320	arg1	terms					194:198	terms	194:198	terms of pectin yield, protein, ash, non-starch polysaccharide, galacturonic acid (GalA), neutral sugar composition, molar mass (Mw), viscosity and degree of branching	194:360	Gold kiwifruit pomace extracted using citric acid, water and enzyme (Celluclast 1.5L) were studied in terms of pectin yield, protein, ash, non-starch polysaccharide, galacturonic acid (GalA), neutral sugar composition, molar mass (Mw), viscosity and degree of branching.
25977029	5	20	theme	nm	766:767	arg1	values					731:736	values	731:736	values	731:736	The Mw and root mean square (RMS) radius varied with the extraction methods with values of 8.4 × 10(5) g/mol and 92 nm, 8.5 × 10(5)g/mol and 102 nm, 6.7 × 10(5) g/mol and 52 nm for acid, water and enzymatic extraction methods, respectively.
25977029	7	21	from	application	1115:1125	arg1	products					1135:1142	food products	1130:1142	food products	1130:1142	This study showed that gold kiwifruit pomace pectin has potential application in food products.
25977029	1	22	theme	Celluclast	161:170	arg1	1.5L					172:175	Celluclast 1.5L	161:175	Celluclast 1.5L	161:175	Gold kiwifruit pomace extracted using citric acid, water and enzyme (Celluclast 1.5L) were studied in terms of pectin yield, protein, ash, non-starch polysaccharide, galacturonic acid (GalA), neutral sugar composition, molar mass (Mw), viscosity and degree of branching.
25977029	1	22	theme	Celluclast	161:170	arg1	acid					137:140	citric acid	130:140	citric acid	130:140	Gold kiwifruit pomace extracted using citric acid, water and enzyme (Celluclast 1.5L) were studied in terms of pectin yield, protein, ash, non-starch polysaccharide, galacturonic acid (GalA), neutral sugar composition, molar mass (Mw), viscosity and degree of branching.
25977029	2	23	theme	Water-extracted	363:377	arg1	pectin					379:384	Water-extracted pectin	363:384	Water-extracted pectin	363:384	Water-extracted pectin was considered closest to its native form.
25977029	6	24	theme	higher	986:991	arg1	viscosity					993:1001	a slightly higher viscosity	975:1001	a slightly higher viscosity followed by acid and enzyme-extracted pectin	975:1046	Similar trend was observed for pectin viscosity, with water-extracted pectin giving a slightly higher viscosity followed by acid and enzyme-extracted pectin.
25977029	3	25	dep	extracted	436:444	arg1	Enzyme					429:434	Enzyme	429:434	Enzyme	429:434	Enzyme extracted pectin showed the highest yield (∼ 4.5%w/w) as compared with the acid and water extraction methods (∼ 3.6-3.8%w/w).
25977029	7	26	theme	gold	1072:1075	arg1	pectin					1094:1099	gold kiwifruit pomace pectin	1072:1099	gold kiwifruit pomace pectin	1072:1099	This study showed that gold kiwifruit pomace pectin has potential application in food products.
25977029	1	27	theme	non-starch	231:240	arg1	polysaccharide					242:255	non-starch polysaccharide	231:255	non-starch polysaccharide	231:255	Gold kiwifruit pomace extracted using citric acid, water and enzyme (Celluclast 1.5L) were studied in terms of pectin yield, protein, ash, non-starch polysaccharide, galacturonic acid (GalA), neutral sugar composition, molar mass (Mw), viscosity and degree of branching.
25977029	1	28	theme	Gold	92:95	arg1	pomace					107:112	Gold kiwifruit pomace	92:112	Gold kiwifruit pomace extracted using citric acid, water and enzyme (Celluclast 1.5L)	92:176	Gold kiwifruit pomace extracted using citric acid, water and enzyme (Celluclast 1.5L) were studied in terms of pectin yield, protein, ash, non-starch polysaccharide, galacturonic acid (GalA), neutral sugar composition, molar mass (Mw), viscosity and degree of branching.
25977029	4	29	theme	different	619:627	arg1	degree					629:634	different degree	619:634	different degree of branching	619:647	Pectin obtained from different extraction methods showed different degree of branching.
25977029	1	30	theme	polysaccharide	242:255	arg1	terms					194:198	terms	194:198	terms of pectin yield, protein, ash, non-starch polysaccharide, galacturonic acid (GalA), neutral sugar composition, molar mass (Mw), viscosity and degree of branching	194:360	Gold kiwifruit pomace extracted using citric acid, water and enzyme (Celluclast 1.5L) were studied in terms of pectin yield, protein, ash, non-starch polysaccharide, galacturonic acid (GalA), neutral sugar composition, molar mass (Mw), viscosity and degree of branching.
25977029	5	31	with	methods	718:724	arg1	values					731:736	values	731:736	values	731:736	The Mw and root mean square (RMS) radius varied with the extraction methods with values of 8.4 × 10(5) g/mol and 92 nm, 8.5 × 10(5)g/mol and 102 nm, 6.7 × 10(5) g/mol and 52 nm for acid, water and enzymatic extraction methods, respectively.
25977029	1	32	theme	kiwifruit	97:105	arg1	pomace					107:112	Gold kiwifruit pomace	92:112	Gold kiwifruit pomace extracted using citric acid, water and enzyme (Celluclast 1.5L)	92:176	Gold kiwifruit pomace extracted using citric acid, water and enzyme (Celluclast 1.5L) were studied in terms of pectin yield, protein, ash, non-starch polysaccharide, galacturonic acid (GalA), neutral sugar composition, molar mass (Mw), viscosity and degree of branching.
25977029	6	33	theme	Similar	891:897	arg1	trend					899:903	Similar trend	891:903	Similar trend	891:903	Similar trend was observed for pectin viscosity, with water-extracted pectin giving a slightly higher viscosity followed by acid and enzyme-extracted pectin.
25977029	0	34	theme	pectin	42:47	arg1	characterisation					15:30	characterisation	15:30	characterisation	15:30	Extraction and characterisation of pomace pectin from gold kiwifruit (Actinidia chinensis).
25977029	0	34	theme	pectin	42:47	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction and characterisation of pomace pectin from gold kiwifruit (Actinidia chinensis).
25977029	5	35	theme	g/mol	753:757	arg1	values					731:736	values	731:736	values	731:736	The Mw and root mean square (RMS) radius varied with the extraction methods with values of 8.4 × 10(5) g/mol and 92 nm, 8.5 × 10(5)g/mol and 102 nm, 6.7 × 10(5) g/mol and 52 nm for acid, water and enzymatic extraction methods, respectively.
25977029	3	36	theme	∼	546:546	arg1	%					555:555	∼ 3.6-3.8%w/w	546:558	∼ 3.6-3.8%w/w	546:558	Enzyme extracted pectin showed the highest yield (∼ 4.5%w/w) as compared with the acid and water extraction methods (∼ 3.6-3.8%w/w).
25977029	3	36	theme	∼	546:546	arg1	acid					511:514	the acid and water extraction methods	507:543	acid	511:514	Enzyme extracted pectin showed the highest yield (∼ 4.5%w/w) as compared with the acid and water extraction methods (∼ 3.6-3.8%w/w).
25977029	5	37	theme	extraction	707:716	arg1	methods					718:724	the extraction methods	703:724	the extraction methods with values of 8.4 × 10(5) g/mol and 92 nm, 8.5 × 10(5)g/mol and 102 nm, 6.7 × 10(5) g/mol and 52 nm for acid, water and enzymatic extraction methods, respectively	703:888	The Mw and root mean square (RMS) radius varied with the extraction methods with values of 8.4 × 10(5) g/mol and 92 nm, 8.5 × 10(5)g/mol and 102 nm, 6.7 × 10(5) g/mol and 52 nm for acid, water and enzymatic extraction methods, respectively.
25977029	0	38	theme	pomace	35:40	arg1	pectin					42:47	pomace pectin	35:47	pomace pectin	35:47	Extraction and characterisation of pomace pectin from gold kiwifruit (Actinidia chinensis).
25977029	1	39	theme	galacturonic	258:269	arg1	acid					271:274	galacturonic acid	258:274	galacturonic acid (GalA)	258:281	Gold kiwifruit pomace extracted using citric acid, water and enzyme (Celluclast 1.5L) were studied in terms of pectin yield, protein, ash, non-starch polysaccharide, galacturonic acid (GalA), neutral sugar composition, molar mass (Mw), viscosity and degree of branching.
25977029	1	39	theme	galacturonic	258:269	arg1	GalA					277:280	GalA	277:280	GalA	277:280	Gold kiwifruit pomace extracted using citric acid, water and enzyme (Celluclast 1.5L) were studied in terms of pectin yield, protein, ash, non-starch polysaccharide, galacturonic acid (GalA), neutral sugar composition, molar mass (Mw), viscosity and degree of branching.
25977029	5	40	theme	mean	666:669	arg1	radius					684:689	The Mw and root mean square (RMS) radius	650:689	radius	684:689	The Mw and root mean square (RMS) radius varied with the extraction methods with values of 8.4 × 10(5) g/mol and 92 nm, 8.5 × 10(5)g/mol and 102 nm, 6.7 × 10(5) g/mol and 52 nm for acid, water and enzymatic extraction methods, respectively.
25977029	3	41	theme	extraction	526:535	arg1	methods					537:543	the acid and water extraction methods	507:543	methods	537:543	Enzyme extracted pectin showed the highest yield (∼ 4.5%w/w) as compared with the acid and water extraction methods (∼ 3.6-3.8%w/w).
25977029	4	42	theme	branching	639:647	arg1	degree					629:634	different degree	619:634	different degree of branching	619:647	Pectin obtained from different extraction methods showed different degree of branching.
25977029	0	43	theme	gold	54:57	arg1	kiwifruit					59:67	gold kiwifruit	54:67	gold kiwifruit (Actinidia chinensis)	54:89	Extraction and characterisation of pomace pectin from gold kiwifruit (Actinidia chinensis).
25977029	0	43	theme	gold	54:57	arg1	chinensis					80:88	Actinidia chinensis	70:88	Actinidia chinensis	70:88	Extraction and characterisation of pomace pectin from gold kiwifruit (Actinidia chinensis).
25977029	1	44	theme	acid	271:274	arg1	terms					194:198	terms	194:198	terms of pectin yield, protein, ash, non-starch polysaccharide, galacturonic acid (GalA), neutral sugar composition, molar mass (Mw), viscosity and degree of branching	194:360	Gold kiwifruit pomace extracted using citric acid, water and enzyme (Celluclast 1.5L) were studied in terms of pectin yield, protein, ash, non-starch polysaccharide, galacturonic acid (GalA), neutral sugar composition, molar mass (Mw), viscosity and degree of branching.
25977029	7	45	theme	potential	1105:1113	arg1	application					1115:1125	potential application	1105:1125	potential application in food products	1105:1142	This study showed that gold kiwifruit pomace pectin has potential application in food products.
25977029	0	46	from	kiwifruit	59:67	arg1	characterisation					15:30	characterisation	15:30	characterisation	15:30	Extraction and characterisation of pomace pectin from gold kiwifruit (Actinidia chinensis).
25977029	0	46	from	kiwifruit	59:67	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction and characterisation of pomace pectin from gold kiwifruit (Actinidia chinensis).
25977029	5	47	theme	×	803:803	arg1	g/mol					811:815	6.7 × 10(5) g/mol	799:815	6.7 × 10(5) g/mol	799:815	The Mw and root mean square (RMS) radius varied with the extraction methods with values of 8.4 × 10(5) g/mol and 92 nm, 8.5 × 10(5)g/mol and 102 nm, 6.7 × 10(5) g/mol and 52 nm for acid, water and enzymatic extraction methods, respectively.
25977029	4	48	theme	extraction	593:602	arg1	methods					604:610	different extraction methods	583:610	different extraction methods	583:610	Pectin obtained from different extraction methods showed different degree of branching.
25977029	1	49	theme	viscosity	328:336	arg1	terms					194:198	terms	194:198	terms of pectin yield, protein, ash, non-starch polysaccharide, galacturonic acid (GalA), neutral sugar composition, molar mass (Mw), viscosity and degree of branching	194:360	Gold kiwifruit pomace extracted using citric acid, water and enzyme (Celluclast 1.5L) were studied in terms of pectin yield, protein, ash, non-starch polysaccharide, galacturonic acid (GalA), neutral sugar composition, molar mass (Mw), viscosity and degree of branching.
25977029	7	50	theme	food	1130:1133	arg1	products					1135:1142	food products	1130:1142	food products	1130:1142	This study showed that gold kiwifruit pomace pectin has potential application in food products.
25977029	4	51	theme	different	583:591	arg1	methods					604:610	different extraction methods	583:610	different extraction methods	583:610	Pectin obtained from different extraction methods showed different degree of branching.
25977029	6	52	theme	water-extracted	945:959	arg1	pectin					961:966	water-extracted pectin	945:966	water-extracted pectin giving a slightly higher viscosity followed by acid and enzyme-extracted pectin	945:1046	Similar trend was observed for pectin viscosity, with water-extracted pectin giving a slightly higher viscosity followed by acid and enzyme-extracted pectin.
25977029	5	53	theme	g/mol	781:785	arg1	values					731:736	values	731:736	values	731:736	The Mw and root mean square (RMS) radius varied with the extraction methods with values of 8.4 × 10(5) g/mol and 92 nm, 8.5 × 10(5)g/mol and 102 nm, 6.7 × 10(5) g/mol and 52 nm for acid, water and enzymatic extraction methods, respectively.
25977029	5	54	theme	nm	824:825	arg1	values					731:736	values	731:736	values	731:736	The Mw and root mean square (RMS) radius varied with the extraction methods with values of 8.4 × 10(5) g/mol and 92 nm, 8.5 × 10(5)g/mol and 102 nm, 6.7 × 10(5) g/mol and 52 nm for acid, water and enzymatic extraction methods, respectively.
25977029	3	55	theme	∼	479:479	arg1	yield					472:476	the highest yield	460:476	the highest yield (∼ 4.5%w/w)	460:488	Enzyme extracted pectin showed the highest yield (∼ 4.5%w/w) as compared with the acid and water extraction methods (∼ 3.6-3.8%w/w).
25977029	3	55	theme	∼	479:479	arg1	%					484:484	∼ 4.5%w/w	479:487	∼ 4.5%w/w	479:487	Enzyme extracted pectin showed the highest yield (∼ 4.5%w/w) as compared with the acid and water extraction methods (∼ 3.6-3.8%w/w).
25977029	7	56	contain	has	1101:1103	arg1	pectin					1094:1099	gold kiwifruit pomace pectin	1072:1099	gold kiwifruit pomace pectin	1072:1099	This study showed that gold kiwifruit pomace pectin has potential application in food products.
25977029	7	56	contain	has	1101:1103	arg2	application					1115:1125	potential application	1105:1125	potential application in food products	1105:1142	This study showed that gold kiwifruit pomace pectin has potential application in food products.
25977029	1	57	theme	degree	342:347	arg1	terms					194:198	terms	194:198	terms of pectin yield, protein, ash, non-starch polysaccharide, galacturonic acid (GalA), neutral sugar composition, molar mass (Mw), viscosity and degree of branching	194:360	Gold kiwifruit pomace extracted using citric acid, water and enzyme (Celluclast 1.5L) were studied in terms of pectin yield, protein, ash, non-starch polysaccharide, galacturonic acid (GalA), neutral sugar composition, molar mass (Mw), viscosity and degree of branching.
25977029	3	58	theme	water	520:524	arg1	methods					537:543	the acid and water extraction methods	507:543	methods	537:543	Enzyme extracted pectin showed the highest yield (∼ 4.5%w/w) as compared with the acid and water extraction methods (∼ 3.6-3.8%w/w).
25977029	1	59	theme	citric	130:135	arg1	1.5L					172:175	Celluclast 1.5L	161:175	Celluclast 1.5L	161:175	Gold kiwifruit pomace extracted using citric acid, water and enzyme (Celluclast 1.5L) were studied in terms of pectin yield, protein, ash, non-starch polysaccharide, galacturonic acid (GalA), neutral sugar composition, molar mass (Mw), viscosity and degree of branching.
25977029	1	59	theme	citric	130:135	arg1	acid					137:140	citric acid	130:140	citric acid	130:140	Gold kiwifruit pomace extracted using citric acid, water and enzyme (Celluclast 1.5L) were studied in terms of pectin yield, protein, ash, non-starch polysaccharide, galacturonic acid (GalA), neutral sugar composition, molar mass (Mw), viscosity and degree of branching.
25977029	5	60	theme	g/mol	811:815	arg1	values					731:736	values	731:736	values	731:736	The Mw and root mean square (RMS) radius varied with the extraction methods with values of 8.4 × 10(5) g/mol and 92 nm, 8.5 × 10(5)g/mol and 102 nm, 6.7 × 10(5) g/mol and 52 nm for acid, water and enzymatic extraction methods, respectively.
25977029	5	61	theme	×	745:745	arg1	g/mol					753:757	8.4 × 10(5) g/mol	741:757	8.4 × 10(5) g/mol	741:757	The Mw and root mean square (RMS) radius varied with the extraction methods with values of 8.4 × 10(5) g/mol and 92 nm, 8.5 × 10(5)g/mol and 102 nm, 6.7 × 10(5) g/mol and 52 nm for acid, water and enzymatic extraction methods, respectively.
25977029	1	62	theme	pectin	203:208	arg1	yield					210:214	pectin yield	203:214	pectin yield	203:214	Gold kiwifruit pomace extracted using citric acid, water and enzyme (Celluclast 1.5L) were studied in terms of pectin yield, protein, ash, non-starch polysaccharide, galacturonic acid (GalA), neutral sugar composition, molar mass (Mw), viscosity and degree of branching.
25977029	5	63	theme	square	671:676	arg1	radius					684:689	The Mw and root mean square (RMS) radius	650:689	radius	684:689	The Mw and root mean square (RMS) radius varied with the extraction methods with values of 8.4 × 10(5) g/mol and 92 nm, 8.5 × 10(5)g/mol and 102 nm, 6.7 × 10(5) g/mol and 52 nm for acid, water and enzymatic extraction methods, respectively.
25977029	5	64	theme	enzymatic	847:855	arg1	methods					868:874	enzymatic extraction methods	847:874	enzymatic extraction methods	847:874	The Mw and root mean square (RMS) radius varied with the extraction methods with values of 8.4 × 10(5) g/mol and 92 nm, 8.5 × 10(5)g/mol and 102 nm, 6.7 × 10(5) g/mol and 52 nm for acid, water and enzymatic extraction methods, respectively.
25977029	1	65	theme	yield	210:214	arg1	terms					194:198	terms	194:198	terms of pectin yield, protein, ash, non-starch polysaccharide, galacturonic acid (GalA), neutral sugar composition, molar mass (Mw), viscosity and degree of branching	194:360	Gold kiwifruit pomace extracted using citric acid, water and enzyme (Celluclast 1.5L) were studied in terms of pectin yield, protein, ash, non-starch polysaccharide, galacturonic acid (GalA), neutral sugar composition, molar mass (Mw), viscosity and degree of branching.
25498207	6	0	theme	molecular	1201:1209	arg1	level					1211:1215	the molecular level	1197:1215	the molecular level	1197:1215	Here we report a molecular model of the wood cell wall material with atomistic resolution, used to assess the mechanical behavior under shear loading in order to understand the deformation mechanisms at the molecular level.
25498207	3	1	theme	cellulose	543:551	arg1	fibers					574:579	stiff fibers	568:579	stiff fibers	568:579	The wood cell wall material features a fiber reinforced composite structure, where cellulose fibrils act as stiff fibers, and hemicellulose and lignin molecules act as soft matrix.
25498207	3	1	theme	cellulose	543:551	arg1	fibrils					553:559	cellulose fibrils	543:559	cellulose fibrils	543:559	The wood cell wall material features a fiber reinforced composite structure, where cellulose fibrils act as stiff fibers, and hemicellulose and lignin molecules act as soft matrix.
25498207	1	2	theme	different	183:191	arg1	scales					193:198	different scales	183:198	different scales	183:198	Wood is a biological material with outstanding mechanical properties resulting from its hierarchical structure across different scales.
25498207	1	3	with	material	86:93	arg1	properties					123:132	outstanding mechanical properties	100:132	outstanding mechanical properties resulting from its hierarchical structure across different scales	100:198	Wood is a biological material with outstanding mechanical properties resulting from its hierarchical structure across different scales.
25498207	3	4	theme	reinforced	505:514	arg1	structure					526:534	a fiber reinforced composite structure	497:534	a fiber reinforced composite structure	497:534	The wood cell wall material features a fiber reinforced composite structure, where cellulose fibrils act as stiff fibers, and hemicellulose and lignin molecules act as soft matrix.
25498207	10	5	theme	behavior	1746:1753	arg1	insights					1719:1726	insights	1719:1726	insights of the mechanical behavior of wood cell wall material	1719:1780	Our molecular dynamics study provides insights of the mechanical behavior of wood cell wall material at the molecular level, and paves a way for the multi-scale understanding of the mechanical properties of wood.
25498207	5	6	theme	overall	866:872	arg1	properties					874:883	the overall properties	862:883	the overall properties	862:883	However, how the interactions between theses constitutive molecules contribute to the overall properties is still unclear, although the shearing between fibers has been proposed as a primary deformation mechanism.
25498207	9	7	dep	mechanisms	1595:1604	arg1	yielding					1607:1614	yielding	1607:1614	the different deformation mechanisms: yielding of the matrix and sliding of matrix along the cellulose surface	1569:1678	The plastic regime can be divided into two parts according to the different deformation mechanisms: yielding of the matrix and sliding of matrix along the cellulose surface.
25498207	9	8	theme	cellulose	1662:1670	arg1	surface					1672:1678	the cellulose surface	1658:1678	the cellulose surface	1658:1678	The plastic regime can be divided into two parts according to the different deformation mechanisms: yielding of the matrix and sliding of matrix along the cellulose surface.
25498207	8	9	theme	wood	1416:1419	arg1	material					1431:1438	the wood cell wall material	1412:1438	the wood cell wall material under shear loading	1412:1458	The results obtained using this model show that the wood cell wall material under shear loading deforms in an elastic and then plastic manner.
25498207	6	10	dep	understand	1156:1165	arg1	to					1153:1154	to	1153:1154	to	1153:1154	Here we report a molecular model of the wood cell wall material with atomistic resolution, used to assess the mechanical behavior under shear loading in order to understand the deformation mechanisms at the molecular level.
25498207	3	11	theme	hemicellulose	586:598	arg1	molecules					611:619	hemicellulose and lignin molecules	586:619	hemicellulose and lignin molecules	586:619	The wood cell wall material features a fiber reinforced composite structure, where cellulose fibrils act as stiff fibers, and hemicellulose and lignin molecules act as soft matrix.
25498207	8	12	theme	wall	1426:1429	arg1	material					1431:1438	the wood cell wall material	1412:1438	the wood cell wall material under shear loading	1412:1458	The results obtained using this model show that the wood cell wall material under shear loading deforms in an elastic and then plastic manner.
25498207	7	13	theme	layered	1341:1347	arg1	nanocomposite					1349:1361	a layered nanocomposite	1339:1361	a layered nanocomposite	1339:1361	The model includes an explicit description of cellulose crystals, hemicellulose, as well as lignin molecules arranged in a layered nanocomposite.
25498207	10	14	theme	wood	1758:1761	arg1	material					1773:1780	wood cell wall material	1758:1780	wood cell wall material	1758:1780	Our molecular dynamics study provides insights of the mechanical behavior of wood cell wall material at the molecular level, and paves a way for the multi-scale understanding of the mechanical properties of wood.
25498207	1	15	theme	mechanical	112:121	arg1	properties					123:132	outstanding mechanical properties	100:132	outstanding mechanical properties resulting from its hierarchical structure across different scales	100:198	Wood is a biological material with outstanding mechanical properties resulting from its hierarchical structure across different scales.
25498207	10	16	theme	molecular	1789:1797	arg1	level					1799:1803	the molecular level	1785:1803	the molecular level	1785:1803	Our molecular dynamics study provides insights of the mechanical behavior of wood cell wall material at the molecular level, and paves a way for the multi-scale understanding of the mechanical properties of wood.
25498207	6	17	theme	deformation	1171:1181	arg1	mechanisms					1183:1192	the deformation mechanisms	1167:1192	the deformation mechanisms	1167:1192	Here we report a molecular model of the wood cell wall material with atomistic resolution, used to assess the mechanical behavior under shear loading in order to understand the deformation mechanisms at the molecular level.
25498207	0	18	theme	material	55:62	arg1	mechanisms					22:31	Molecular deformation mechanisms	0:31	Molecular deformation mechanisms of the wood cell wall material	0:62	Molecular deformation mechanisms of the wood cell wall material.
25498207	3	19	theme	lignin	604:609	arg1	molecules					611:619	hemicellulose and lignin molecules	586:619	hemicellulose and lignin molecules	586:619	The wood cell wall material features a fiber reinforced composite structure, where cellulose fibrils act as stiff fibers, and hemicellulose and lignin molecules act as soft matrix.
25498207	8	20	theme	elastic	1474:1480	arg1	manner					1499:1504	an elastic and then plastic manner	1471:1504	an elastic and then plastic manner	1471:1504	The results obtained using this model show that the wood cell wall material under shear loading deforms in an elastic and then plastic manner.
25498207	9	21	theme	plastic	1511:1517	arg1	regime					1519:1524	The plastic regime	1507:1524	The plastic regime	1507:1524	The plastic regime can be divided into two parts according to the different deformation mechanisms: yielding of the matrix and sliding of matrix along the cellulose surface.
25498207	2	22	theme	cell	388:391	arg1	material					398:405	the wood cell wall material	379:405	the wood cell wall material itself	379:412	Although earlier work has shown that the cellular structure of wood is a key factor that renders it excellent mechanical properties at light weight, the mechanical properties of the wood cell wall material itself still needs to be understood comprehensively.
25498207	5	23	theme	primary	963:969	arg1	mechanism					983:991	a primary deformation mechanism	961:991	a primary deformation mechanism	961:991	However, how the interactions between theses constitutive molecules contribute to the overall properties is still unclear, although the shearing between fibers has been proposed as a primary deformation mechanism.
25498207	5	23	theme	primary	963:969	arg1	shearing					916:923	the shearing	912:923	the shearing between fibers	912:938	However, how the interactions between theses constitutive molecules contribute to the overall properties is still unclear, although the shearing between fibers has been proposed as a primary deformation mechanism.
25498207	6	24	theme	material	1049:1056	arg1	model					1021:1025	a molecular model	1009:1025	a molecular model of the wood cell wall material with atomistic resolution, used to assess the mechanical behavior under shear loading in order to understand the deformation mechanisms at the molecular level	1009:1215	Here we report a molecular model of the wood cell wall material with atomistic resolution, used to assess the mechanical behavior under shear loading in order to understand the deformation mechanisms at the molecular level.
25498207	3	25	theme	composite	516:524	arg1	structure					526:534	a fiber reinforced composite structure	497:534	a fiber reinforced composite structure	497:534	The wood cell wall material features a fiber reinforced composite structure, where cellulose fibrils act as stiff fibers, and hemicellulose and lignin molecules act as soft matrix.
25498207	0	26	theme	Molecular	0:8	arg1	mechanisms					22:31	Molecular deformation mechanisms	0:31	Molecular deformation mechanisms of the wood cell wall material	0:62	Molecular deformation mechanisms of the wood cell wall material.
25498207	1	27	theme	biological	75:84	arg1	Wood					65:68	Wood	65:68	Wood	65:68	Wood is a biological material with outstanding mechanical properties resulting from its hierarchical structure across different scales.
25498207	1	27	theme	biological	75:84	arg1	material					86:93	a biological material	73:93	a biological material with outstanding mechanical properties resulting from its hierarchical structure across different scales	73:198	Wood is a biological material with outstanding mechanical properties resulting from its hierarchical structure across different scales.
25498207	6	28	theme	cell	1039:1042	arg1	material					1049:1056	the wood cell wall material	1030:1056	the wood cell wall material	1030:1056	Here we report a molecular model of the wood cell wall material with atomistic resolution, used to assess the mechanical behavior under shear loading in order to understand the deformation mechanisms at the molecular level.
25498207	2	29	theme	material	398:405	arg1	properties					365:374	the mechanical properties	350:374	the mechanical properties of the wood cell wall material itself	350:412	Although earlier work has shown that the cellular structure of wood is a key factor that renders it excellent mechanical properties at light weight, the mechanical properties of the wood cell wall material itself still needs to be understood comprehensively.
25498207	3	30	theme	soft	628:631	arg1	matrix					633:638	soft matrix	628:638	soft matrix	628:638	The wood cell wall material features a fiber reinforced composite structure, where cellulose fibrils act as stiff fibers, and hemicellulose and lignin molecules act as soft matrix.
25498207	0	31	theme	cell	45:48	arg1	material					55:62	the wood cell wall material	36:62	the wood cell wall material	36:62	Molecular deformation mechanisms of the wood cell wall material.
25498207	7	32	theme	cellulose	1264:1272	arg1	crystals					1274:1281	cellulose crystals	1264:1281	cellulose crystals	1264:1281	The model includes an explicit description of cellulose crystals, hemicellulose, as well as lignin molecules arranged in a layered nanocomposite.
25498207	3	33	theme	cell	469:472	arg1	material					479:486	The wood cell wall material	460:486	The wood cell wall material	460:486	The wood cell wall material features a fiber reinforced composite structure, where cellulose fibrils act as stiff fibers, and hemicellulose and lignin molecules act as soft matrix.
25498207	9	34	theme	deformation	1583:1593	arg1	mechanisms					1595:1604	the different deformation mechanisms	1569:1604	the different deformation mechanisms: yielding of the matrix and sliding of matrix along the cellulose surface	1569:1678	The plastic regime can be divided into two parts according to the different deformation mechanisms: yielding of the matrix and sliding of matrix along the cellulose surface.
25498207	10	35	theme	dynamics	1695:1702	arg1	study					1704:1708	Our molecular dynamics study	1681:1708	Our molecular dynamics study	1681:1708	Our molecular dynamics study provides insights of the mechanical behavior of wood cell wall material at the molecular level, and paves a way for the multi-scale understanding of the mechanical properties of wood.
25498207	6	36	theme	atomistic	1063:1071	arg1	resolution					1073:1082	atomistic resolution	1063:1082	atomistic resolution	1063:1082	Here we report a molecular model of the wood cell wall material with atomistic resolution, used to assess the mechanical behavior under shear loading in order to understand the deformation mechanisms at the molecular level.
25498207	9	37	theme	matrix	1623:1628	arg1	yielding					1607:1614	yielding	1607:1614	the different deformation mechanisms: yielding of the matrix and sliding of matrix along the cellulose surface	1569:1678	The plastic regime can be divided into two parts according to the different deformation mechanisms: yielding of the matrix and sliding of matrix along the cellulose surface.
25498207	2	38	from	weight	342:347	arg1	excellent					301:309	excellent	301:309	excellent	301:309	Although earlier work has shown that the cellular structure of wood is a key factor that renders it excellent mechanical properties at light weight, the mechanical properties of the wood cell wall material itself still needs to be understood comprehensively.
25498207	10	39	theme	wood	1888:1891	arg1	properties					1874:1883	the mechanical properties	1859:1883	the mechanical properties of wood	1859:1891	Our molecular dynamics study provides insights of the mechanical behavior of wood cell wall material at the molecular level, and paves a way for the multi-scale understanding of the mechanical properties of wood.
25498207	2	40	theme	key	274:276	arg1	factor					278:283	a key factor	272:283	a key factor that renders it excellent mechanical properties at light weight	272:347	Although earlier work has shown that the cellular structure of wood is a key factor that renders it excellent mechanical properties at light weight, the mechanical properties of the wood cell wall material itself still needs to be understood comprehensively.
25498207	2	40	theme	key	274:276	arg1	structure					251:259	the cellular structure	238:259	the cellular structure of wood	238:267	Although earlier work has shown that the cellular structure of wood is a key factor that renders it excellent mechanical properties at light weight, the mechanical properties of the wood cell wall material itself still needs to be understood comprehensively.
25498207	9	41	theme	sliding	1634:1640	arg1	yielding					1607:1614	yielding	1607:1614	the different deformation mechanisms: yielding of the matrix and sliding of matrix along the cellulose surface	1569:1678	The plastic regime can be divided into two parts according to the different deformation mechanisms: yielding of the matrix and sliding of matrix along the cellulose surface.
25498207	10	42	theme	properties	1874:1883	arg1	understanding					1842:1854	the multi-scale understanding	1826:1854	the multi-scale understanding of the mechanical properties of wood	1826:1891	Our molecular dynamics study provides insights of the mechanical behavior of wood cell wall material at the molecular level, and paves a way for the multi-scale understanding of the mechanical properties of wood.
25498207	2	43	theme	mechanical	311:320	arg1	properties					322:331	mechanical properties	311:331	mechanical properties	311:331	Although earlier work has shown that the cellular structure of wood is a key factor that renders it excellent mechanical properties at light weight, the mechanical properties of the wood cell wall material itself still needs to be understood comprehensively.
25498207	9	44	theme	matrix	1645:1650	arg1	sliding					1634:1640	the matrix and sliding	1619:1640	sliding	1634:1640	The plastic regime can be divided into two parts according to the different deformation mechanisms: yielding of the matrix and sliding of matrix along the cellulose surface.
25498207	9	44	theme	matrix	1645:1650	arg1	matrix					1623:1628	the matrix and sliding	1619:1640	matrix	1623:1628	The plastic regime can be divided into two parts according to the different deformation mechanisms: yielding of the matrix and sliding of matrix along the cellulose surface.
25498207	6	45	theme	shear	1130:1134	arg1	loading					1136:1142	shear loading	1130:1142	shear loading	1130:1142	Here we report a molecular model of the wood cell wall material with atomistic resolution, used to assess the mechanical behavior under shear loading in order to understand the deformation mechanisms at the molecular level.
25498207	10	46	theme	mechanical	1735:1744	arg1	behavior					1746:1753	the mechanical behavior	1731:1753	the mechanical behavior of wood cell wall material	1731:1780	Our molecular dynamics study provides insights of the mechanical behavior of wood cell wall material at the molecular level, and paves a way for the multi-scale understanding of the mechanical properties of wood.
25498207	3	47	theme	stiff	568:572	arg1	fibers					574:579	stiff fibers	568:579	stiff fibers	568:579	The wood cell wall material features a fiber reinforced composite structure, where cellulose fibrils act as stiff fibers, and hemicellulose and lignin molecules act as soft matrix.
25498207	3	47	theme	stiff	568:572	arg1	fibrils					553:559	cellulose fibrils	543:559	cellulose fibrils	543:559	The wood cell wall material features a fiber reinforced composite structure, where cellulose fibrils act as stiff fibers, and hemicellulose and lignin molecules act as soft matrix.
25498207	8	48	theme	cell	1421:1424	arg1	material					1431:1438	the wood cell wall material	1412:1438	the wood cell wall material under shear loading	1412:1458	The results obtained using this model show that the wood cell wall material under shear loading deforms in an elastic and then plastic manner.
25498207	10	49	theme	material	1773:1780	arg1	behavior					1746:1753	the mechanical behavior	1731:1753	the mechanical behavior of wood cell wall material	1731:1780	Our molecular dynamics study provides insights of the mechanical behavior of wood cell wall material at the molecular level, and paves a way for the multi-scale understanding of the mechanical properties of wood.
25498207	5	50	theme	constitutive	825:836	arg1	molecules					838:846	theses constitutive molecules	818:846	theses constitutive molecules	818:846	However, how the interactions between theses constitutive molecules contribute to the overall properties is still unclear, although the shearing between fibers has been proposed as a primary deformation mechanism.
25498207	10	51	theme	cell	1763:1766	arg1	material					1773:1780	wood cell wall material	1758:1780	wood cell wall material	1758:1780	Our molecular dynamics study provides insights of the mechanical behavior of wood cell wall material at the molecular level, and paves a way for the multi-scale understanding of the mechanical properties of wood.
25498207	1	52	theme	outstanding	100:110	arg1	properties					123:132	outstanding mechanical properties	100:132	outstanding mechanical properties resulting from its hierarchical structure across different scales	100:198	Wood is a biological material with outstanding mechanical properties resulting from its hierarchical structure across different scales.
25498207	8	53	theme	shear	1446:1450	arg1	loading					1452:1458	shear loading	1446:1458	shear loading	1446:1458	The results obtained using this model show that the wood cell wall material under shear loading deforms in an elastic and then plastic manner.
25498207	4	54	theme	loading	687:693	arg1	direction					695:703	the loading direction	683:703	the loading direction	683:703	The angle between the fiber direction and the loading direction has been found to be the key factor controlling the mechanical properties.
25498207	5	55	theme	deformation	971:981	arg1	mechanism					983:991	a primary deformation mechanism	961:991	a primary deformation mechanism	961:991	However, how the interactions between theses constitutive molecules contribute to the overall properties is still unclear, although the shearing between fibers has been proposed as a primary deformation mechanism.
25498207	5	55	theme	deformation	971:981	arg1	shearing					916:923	the shearing	912:923	the shearing between fibers	912:938	However, how the interactions between theses constitutive molecules contribute to the overall properties is still unclear, although the shearing between fibers has been proposed as a primary deformation mechanism.
25498207	6	56	theme	molecular	1011:1019	arg1	model					1021:1025	a molecular model	1009:1025	a molecular model of the wood cell wall material with atomistic resolution, used to assess the mechanical behavior under shear loading in order to understand the deformation mechanisms at the molecular level	1009:1215	Here we report a molecular model of the wood cell wall material with atomistic resolution, used to assess the mechanical behavior under shear loading in order to understand the deformation mechanisms at the molecular level.
25498207	4	57	theme	fiber	663:667	arg1	direction					669:677	the fiber direction	659:677	the fiber direction	659:677	The angle between the fiber direction and the loading direction has been found to be the key factor controlling the mechanical properties.
25498207	2	58	theme	wood	264:267	arg1	factor					278:283	a key factor	272:283	a key factor that renders it excellent mechanical properties at light weight	272:347	Although earlier work has shown that the cellular structure of wood is a key factor that renders it excellent mechanical properties at light weight, the mechanical properties of the wood cell wall material itself still needs to be understood comprehensively.
25498207	2	58	theme	wood	264:267	arg1	structure					251:259	the cellular structure	238:259	the cellular structure of wood	238:267	Although earlier work has shown that the cellular structure of wood is a key factor that renders it excellent mechanical properties at light weight, the mechanical properties of the wood cell wall material itself still needs to be understood comprehensively.
25498207	1	59	theme	hierarchical	153:164	arg1	structure					166:174	its hierarchical structure	149:174	its hierarchical structure across different scales	149:198	Wood is a biological material with outstanding mechanical properties resulting from its hierarchical structure across different scales.
25498207	8	60	theme	plastic	1491:1497	arg1	manner					1499:1504	an elastic and then plastic manner	1471:1504	an elastic and then plastic manner	1471:1504	The results obtained using this model show that the wood cell wall material under shear loading deforms in an elastic and then plastic manner.
25498207	4	61	theme	key	730:732	arg1	factor					734:739	the key factor	726:739	the key factor controlling the mechanical properties	726:777	The angle between the fiber direction and the loading direction has been found to be the key factor controlling the mechanical properties.
25498207	4	61	theme	key	730:732	arg1	angle					645:649	The angle	641:649	The angle between the fiber direction and the loading direction	641:703	The angle between the fiber direction and the loading direction has been found to be the key factor controlling the mechanical properties.
25498207	2	62	theme	wood	383:386	arg1	material					398:405	the wood cell wall material	379:405	the wood cell wall material itself	379:412	Although earlier work has shown that the cellular structure of wood is a key factor that renders it excellent mechanical properties at light weight, the mechanical properties of the wood cell wall material itself still needs to be understood comprehensively.
25498207	0	63	theme	deformation	10:20	arg1	mechanisms					22:31	Molecular deformation mechanisms	0:31	Molecular deformation mechanisms of the wood cell wall material	0:62	Molecular deformation mechanisms of the wood cell wall material.
25498207	6	64	theme	wall	1044:1047	arg1	material					1049:1056	the wood cell wall material	1030:1056	the wood cell wall material	1030:1056	Here we report a molecular model of the wood cell wall material with atomistic resolution, used to assess the mechanical behavior under shear loading in order to understand the deformation mechanisms at the molecular level.
25498207	2	65	theme	cellular	242:249	arg1	factor					278:283	a key factor	272:283	a key factor that renders it excellent mechanical properties at light weight	272:347	Although earlier work has shown that the cellular structure of wood is a key factor that renders it excellent mechanical properties at light weight, the mechanical properties of the wood cell wall material itself still needs to be understood comprehensively.
25498207	2	65	theme	cellular	242:249	arg1	structure					251:259	the cellular structure	238:259	the cellular structure of wood	238:267	Although earlier work has shown that the cellular structure of wood is a key factor that renders it excellent mechanical properties at light weight, the mechanical properties of the wood cell wall material itself still needs to be understood comprehensively.
25498207	6	66	theme	wood	1034:1037	arg1	material					1049:1056	the wood cell wall material	1030:1056	the wood cell wall material	1030:1056	Here we report a molecular model of the wood cell wall material with atomistic resolution, used to assess the mechanical behavior under shear loading in order to understand the deformation mechanisms at the molecular level.
25498207	3	67	theme	wood	464:467	arg1	material					479:486	The wood cell wall material	460:486	The wood cell wall material	460:486	The wood cell wall material features a fiber reinforced composite structure, where cellulose fibrils act as stiff fibers, and hemicellulose and lignin molecules act as soft matrix.
25498207	0	68	theme	wood	40:43	arg1	material					55:62	the wood cell wall material	36:62	the wood cell wall material	36:62	Molecular deformation mechanisms of the wood cell wall material.
25498207	7	69	theme	crystals	1274:1281	arg1	hemicellulose					1284:1296	hemicellulose	1284:1296	hemicellulose	1284:1296	The model includes an explicit description of cellulose crystals, hemicellulose, as well as lignin molecules arranged in a layered nanocomposite.
25498207	7	69	theme	crystals	1274:1281	arg1	description					1249:1259	an explicit description	1237:1259	an explicit description of cellulose crystals	1237:1281	The model includes an explicit description of cellulose crystals, hemicellulose, as well as lignin molecules arranged in a layered nanocomposite.
25498207	7	69	theme	crystals	1274:1281	arg1	molecules					1317:1325	lignin molecules	1310:1325	an explicit description of cellulose crystals, hemicellulose, as well as lignin molecules arranged in a layered nanocomposite	1237:1361	The model includes an explicit description of cellulose crystals, hemicellulose, as well as lignin molecules arranged in a layered nanocomposite.
25498207	2	70	theme	wall	393:396	arg1	material					398:405	the wood cell wall material	379:405	the wood cell wall material itself	379:412	Although earlier work has shown that the cellular structure of wood is a key factor that renders it excellent mechanical properties at light weight, the mechanical properties of the wood cell wall material itself still needs to be understood comprehensively.
25498207	0	71	theme	wall	50:53	arg1	material					55:62	the wood cell wall material	36:62	the wood cell wall material	36:62	Molecular deformation mechanisms of the wood cell wall material.
25498207	6	72	with	material	1049:1056	arg1	resolution					1073:1082	atomistic resolution	1063:1082	atomistic resolution	1063:1082	Here we report a molecular model of the wood cell wall material with atomistic resolution, used to assess the mechanical behavior under shear loading in order to understand the deformation mechanisms at the molecular level.
25498207	6	73	theme	mechanical	1104:1113	arg1	behavior					1115:1122	the mechanical behavior	1100:1122	the mechanical behavior	1100:1122	Here we report a molecular model of the wood cell wall material with atomistic resolution, used to assess the mechanical behavior under shear loading in order to understand the deformation mechanisms at the molecular level.
25498207	2	74	theme	mechanical	354:363	arg1	properties					365:374	the mechanical properties	350:374	the mechanical properties of the wood cell wall material itself	350:412	Although earlier work has shown that the cellular structure of wood is a key factor that renders it excellent mechanical properties at light weight, the mechanical properties of the wood cell wall material itself still needs to be understood comprehensively.
25498207	10	75	theme	multi-scale	1830:1840	arg1	understanding					1842:1854	the multi-scale understanding	1826:1854	the multi-scale understanding of the mechanical properties of wood	1826:1891	Our molecular dynamics study provides insights of the mechanical behavior of wood cell wall material at the molecular level, and paves a way for the multi-scale understanding of the mechanical properties of wood.
25498207	2	76	theme	light	336:340	arg1	weight					342:347	light weight	336:347	light weight	336:347	Although earlier work has shown that the cellular structure of wood is a key factor that renders it excellent mechanical properties at light weight, the mechanical properties of the wood cell wall material itself still needs to be understood comprehensively.
25498207	9	77	theme	different	1573:1581	arg1	mechanisms					1595:1604	the different deformation mechanisms	1569:1604	the different deformation mechanisms: yielding of the matrix and sliding of matrix along the cellulose surface	1569:1678	The plastic regime can be divided into two parts according to the different deformation mechanisms: yielding of the matrix and sliding of matrix along the cellulose surface.
25498207	10	78	theme	wall	1768:1771	arg1	material					1773:1780	wood cell wall material	1758:1780	wood cell wall material	1758:1780	Our molecular dynamics study provides insights of the mechanical behavior of wood cell wall material at the molecular level, and paves a way for the multi-scale understanding of the mechanical properties of wood.
25498207	7	79	theme	explicit	1240:1247	arg1	hemicellulose					1284:1296	hemicellulose	1284:1296	hemicellulose	1284:1296	The model includes an explicit description of cellulose crystals, hemicellulose, as well as lignin molecules arranged in a layered nanocomposite.
25498207	7	79	theme	explicit	1240:1247	arg1	description					1249:1259	an explicit description	1237:1259	an explicit description of cellulose crystals	1237:1281	The model includes an explicit description of cellulose crystals, hemicellulose, as well as lignin molecules arranged in a layered nanocomposite.
25498207	2	80	theme	earlier	210:216	arg1	work					218:221	earlier work	210:221	earlier work	210:221	Although earlier work has shown that the cellular structure of wood is a key factor that renders it excellent mechanical properties at light weight, the mechanical properties of the wood cell wall material itself still needs to be understood comprehensively.
25498207	4	81	theme	mechanical	757:766	arg1	properties					768:777	the mechanical properties	753:777	the mechanical properties	753:777	The angle between the fiber direction and the loading direction has been found to be the key factor controlling the mechanical properties.
25498207	3	82	theme	wall	474:477	arg1	material					479:486	The wood cell wall material	460:486	The wood cell wall material	460:486	The wood cell wall material features a fiber reinforced composite structure, where cellulose fibrils act as stiff fibers, and hemicellulose and lignin molecules act as soft matrix.
25498207	7	83	theme	lignin	1310:1315	arg1	molecules					1317:1325	lignin molecules	1310:1325	an explicit description of cellulose crystals, hemicellulose, as well as lignin molecules arranged in a layered nanocomposite	1237:1361	The model includes an explicit description of cellulose crystals, hemicellulose, as well as lignin molecules arranged in a layered nanocomposite.
25498207	10	84	theme	mechanical	1863:1872	arg1	properties					1874:1883	the mechanical properties	1859:1883	the mechanical properties of wood	1859:1891	Our molecular dynamics study provides insights of the mechanical behavior of wood cell wall material at the molecular level, and paves a way for the multi-scale understanding of the mechanical properties of wood.
25498207	10	85	theme	molecular	1685:1693	arg1	dynamics					1695:1702	molecular dynamics	1685:1702	Our molecular dynamics study	1681:1708	Our molecular dynamics study provides insights of the mechanical behavior of wood cell wall material at the molecular level, and paves a way for the multi-scale understanding of the mechanical properties of wood.
26428145	0	0	theme	polysaccharide/polyester	89:112	arg1	membranes					114:122	chitosan-based polysaccharide/polyester membranes	74:122	chitosan-based polysaccharide/polyester membranes	74:122	Antibacterial activity and in vitro evaluation of the biocompatibility of chitosan-based polysaccharide/polyester membranes.
26428145	8	1	theme	CS-based	1264:1271	arg1	polysaccharide					1273:1286	CS-based polysaccharide	1264:1286	CS-based polysaccharide	1264:1286	Membranes of PHBV-g-MA or PHBV containing CS-based polysaccharide had better antibacterial activity.
26428145	1	2	theme	poly	189:192	arg1	/CS					265:267	poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and chitosan (CS) (PHBV)/CS)	189:268	poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and chitosan (CS) (PHBV)/CS)	189:268	The antibacterial activity and biocompatibility of membranes of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and chitosan (CS) (PHBV)/CS) were evaluated in this study.
26428145	0	3	theme	chitosan-based	74:87	arg1	membranes					114:122	chitosan-based polysaccharide/polyester membranes	74:122	chitosan-based polysaccharide/polyester membranes	74:122	Antibacterial activity and in vitro evaluation of the biocompatibility of chitosan-based polysaccharide/polyester membranes.
26428145	3	4	theme	films	473:477	arg1	series					457:462	two series	453:462	two series of these films to assess cytocompatibility	453:505	Mouse tail skin fibroblasts (FBs) were seeded on two series of these films to assess cytocompatibility.
26428145	1	5	theme	/CS	265:267	arg1	membranes					176:184	membranes	176:184	membranes of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and chitosan (CS) (PHBV)/CS)	176:268	The antibacterial activity and biocompatibility of membranes of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and chitosan (CS) (PHBV)/CS) were evaluated in this study.
26428145	7	6	theme	membranes	1211:1219	arg1	BCRC					1169:1172	BCRC 10239	1169:1178	BCRC 10239	1169:1178	Moreover, CS-based polysaccharide enhanced the Escherichia coli (BCRC 10239) antibacterial activity of the membranes.
26428145	7	6	theme	membranes	1211:1219	arg1	activity					1195:1202	the Escherichia coli (BCRC 10239) antibacterial activity	1147:1202	the Escherichia coli (BCRC 10239) antibacterial activity of the membranes	1147:1219	Moreover, CS-based polysaccharide enhanced the Escherichia coli (BCRC 10239) antibacterial activity of the membranes.
26428145	7	7	theme	CS-based	1114:1121	arg1	polysaccharide					1123:1136	CS-based polysaccharide	1114:1136	CS-based polysaccharide	1114:1136	Moreover, CS-based polysaccharide enhanced the Escherichia coli (BCRC 10239) antibacterial activity of the membranes.
26428145	0	8	theme	membranes	114:122	arg1	biocompatibility					54:69	the biocompatibility	50:69	the biocompatibility of chitosan-based polysaccharide/polyester membranes	50:122	Antibacterial activity and in vitro evaluation of the biocompatibility of chitosan-based polysaccharide/polyester membranes.
26428145	7	9	dep	Escherichia	1151:1161	arg1	coli					1163:1166	coli	1163:1166	coli	1163:1166	Moreover, CS-based polysaccharide enhanced the Escherichia coli (BCRC 10239) antibacterial activity of the membranes.
26428145	6	10	theme	material	1081:1088	arg1	applications					1090:1101	biomedical material applications	1070:1101	biomedical material applications	1070:1101	Cell-cycle and apoptosis assays by FBs on the PHBV-series membrane samples were not affected by DNA content related to damage; i.e. rapid apoptosis/necrosis was not observed, demonstrating the potential of PHBV/CS or PHBV-g-MA/CS membranes for biomedical material applications.
26428145	4	11	theme	FB	645:646	arg1	proliferation					648:660	FB proliferation	645:660	FB proliferation	645:660	Collagen and cell proliferation analyses indicated that PHBV, PHBV-g-MA and their composite membranes were biocompatible with respect to FB proliferation.
26428145	5	12	theme	normal	732:737	arg1	cells					739:743	normal cells	732:743	normal cells growing on PHBV/CS membranes	732:772	However, FB proliferation, collagen production and the percentage of normal cells growing on PHBV/CS membranes were greater than those for PHBV-g-MA/CS membranes.
26428145	6	13	theme	PHBV-series	872:882	arg1	membrane					884:891	the PHBV-series membrane	868:891	the PHBV-series membrane	868:891	Cell-cycle and apoptosis assays by FBs on the PHBV-series membrane samples were not affected by DNA content related to damage; i.e. rapid apoptosis/necrosis was not observed, demonstrating the potential of PHBV/CS or PHBV-g-MA/CS membranes for biomedical material applications.
26428145	6	14	theme	assays	851:856	arg1	samples					893:899	Cell-cycle and apoptosis assays by FBs on the PHBV-series membrane samples	826:899	Cell-cycle and apoptosis assays by FBs on the PHBV-series membrane samples	826:899	Cell-cycle and apoptosis assays by FBs on the PHBV-series membrane samples were not affected by DNA content related to damage; i.e. rapid apoptosis/necrosis was not observed, demonstrating the potential of PHBV/CS or PHBV-g-MA/CS membranes for biomedical material applications.
26428145	6	15	from	assays	851:856	arg1	membrane					884:891	the PHBV-series membrane	868:891	the PHBV-series membrane	868:891	Cell-cycle and apoptosis assays by FBs on the PHBV-series membrane samples were not affected by DNA content related to damage; i.e. rapid apoptosis/necrosis was not observed, demonstrating the potential of PHBV/CS or PHBV-g-MA/CS membranes for biomedical material applications.
26428145	6	16	theme	biomedical	1070:1079	arg1	applications					1090:1101	biomedical material applications	1070:1101	biomedical material applications	1070:1101	Cell-cycle and apoptosis assays by FBs on the PHBV-series membrane samples were not affected by DNA content related to damage; i.e. rapid apoptosis/necrosis was not observed, demonstrating the potential of PHBV/CS or PHBV-g-MA/CS membranes for biomedical material applications.
26428145	7	17	theme	antibacterial	1181:1193	arg1	BCRC					1169:1172	BCRC 10239	1169:1178	BCRC 10239	1169:1178	Moreover, CS-based polysaccharide enhanced the Escherichia coli (BCRC 10239) antibacterial activity of the membranes.
26428145	7	17	theme	antibacterial	1181:1193	arg1	activity					1195:1202	the Escherichia coli (BCRC 10239) antibacterial activity	1147:1202	the Escherichia coli (BCRC 10239) antibacterial activity of the membranes	1147:1219	Moreover, CS-based polysaccharide enhanced the Escherichia coli (BCRC 10239) antibacterial activity of the membranes.
26428145	5	18	theme	PHBV/CS	756:762	arg1	membranes					764:772	PHBV/CS membranes	756:772	PHBV/CS membranes	756:772	However, FB proliferation, collagen production and the percentage of normal cells growing on PHBV/CS membranes were greater than those for PHBV-g-MA/CS membranes.
26428145	6	19	theme	rapid	958:962	arg1	apoptosis/necrosis					964:981	i.e. rapid apoptosis/necrosis	953:981	i.e. rapid apoptosis/necrosis	953:981	Cell-cycle and apoptosis assays by FBs on the PHBV-series membrane samples were not affected by DNA content related to damage; i.e. rapid apoptosis/necrosis was not observed, demonstrating the potential of PHBV/CS or PHBV-g-MA/CS membranes for biomedical material applications.
26428145	3	20	theme	tail	410:413	arg1	fibroblasts					420:430	Mouse tail skin fibroblasts	404:430	Mouse tail skin fibroblasts (FBs)	404:436	Mouse tail skin fibroblasts (FBs) were seeded on two series of these films to assess cytocompatibility.
26428145	3	20	theme	tail	410:413	arg1	FBs					433:435	FBs	433:435	FBs	433:435	Mouse tail skin fibroblasts (FBs) were seeded on two series of these films to assess cytocompatibility.
26428145	0	21	theme	Antibacterial	0:12	arg1	activity					14:21	Antibacterial activity	0:21	Antibacterial activity	0:21	Antibacterial activity and in vitro evaluation of the biocompatibility of chitosan-based polysaccharide/polyester membranes.
26428145	4	22	theme	proliferation	526:538	arg1	analyses					540:547	Collagen and cell proliferation analyses	508:547	Collagen and cell proliferation analyses	508:547	Collagen and cell proliferation analyses indicated that PHBV, PHBV-g-MA and their composite membranes were biocompatible with respect to FB proliferation.
26428145	8	23	contain	containing	1253:1262	arg2	polysaccharide					1273:1286	CS-based polysaccharide	1264:1286	CS-based polysaccharide	1264:1286	Membranes of PHBV-g-MA or PHBV containing CS-based polysaccharide had better antibacterial activity.
26428145	8	23	contain	containing	1253:1262	arg1	PHBV-g-MA					1235:1243	PHBV-g-MA	1235:1243	PHBV-g-MA	1235:1243	Membranes of PHBV-g-MA or PHBV containing CS-based polysaccharide had better antibacterial activity.
26428145	2	24	theme	anhydride	307:315	arg1	PHBV-g-MA					352:360	PHBV-g-MA	352:360	PHBV-g-MA	352:360	Maleic anhydride (MA)-grafted polyhydroxyalkanoate (PHBV-g-MA) was evaluated as an alternative to PHBV.
26428145	2	24	theme	anhydride	307:315	arg1	polyhydroxyalkanoate					330:349	Maleic anhydride (MA)-grafted polyhydroxyalkanoate	300:349	Maleic anhydride (MA)-grafted polyhydroxyalkanoate (PHBV-g-MA)	300:361	Maleic anhydride (MA)-grafted polyhydroxyalkanoate (PHBV-g-MA) was evaluated as an alternative to PHBV.
26428145	2	24	theme	anhydride	307:315	arg1	alternative					383:393	an alternative	380:393	an alternative to PHBV	380:401	Maleic anhydride (MA)-grafted polyhydroxyalkanoate (PHBV-g-MA) was evaluated as an alternative to PHBV.
26428145	8	25	contain	had	1288:1290	arg2	activity					1313:1320	better antibacterial activity	1292:1320	better antibacterial activity	1292:1320	Membranes of PHBV-g-MA or PHBV containing CS-based polysaccharide had better antibacterial activity.
26428145	8	25	contain	had	1288:1290	arg1	Membranes					1222:1230	Membranes	1222:1230	Membranes of PHBV-g-MA or PHBV containing CS-based polysaccharide	1222:1286	Membranes of PHBV-g-MA or PHBV containing CS-based polysaccharide had better antibacterial activity.
26428145	3	26	theme	skin	415:418	arg1	fibroblasts					420:430	Mouse tail skin fibroblasts	404:430	Mouse tail skin fibroblasts (FBs)	404:436	Mouse tail skin fibroblasts (FBs) were seeded on two series of these films to assess cytocompatibility.
26428145	3	26	theme	skin	415:418	arg1	FBs					433:435	FBs	433:435	FBs	433:435	Mouse tail skin fibroblasts (FBs) were seeded on two series of these films to assess cytocompatibility.
26428145	0	27	theme	in	27:28	arg1	evaluation					36:45	in vitro evaluation	27:45	in vitro evaluation of the biocompatibility of chitosan-based polysaccharide/polyester membranes	27:122	Antibacterial activity and in vitro evaluation of the biocompatibility of chitosan-based polysaccharide/polyester membranes.
26428145	4	28	theme	cell	521:524	arg1	proliferation					526:538	cell proliferation	521:538	cell proliferation	521:538	Collagen and cell proliferation analyses indicated that PHBV, PHBV-g-MA and their composite membranes were biocompatible with respect to FB proliferation.
26428145	2	29	theme	Maleic	300:305	arg1	MA					318:319	MA	318:319	MA	318:319	Maleic anhydride (MA)-grafted polyhydroxyalkanoate (PHBV-g-MA) was evaluated as an alternative to PHBV.
26428145	2	29	theme	Maleic	300:305	arg1	anhydride					307:315	Maleic anhydride	300:315	Maleic anhydride (MA)-grafted polyhydroxyalkanoate (PHBV-g-MA)	300:361	Maleic anhydride (MA)-grafted polyhydroxyalkanoate (PHBV-g-MA) was evaluated as an alternative to PHBV.
26428145	6	30	theme	PHBV-g-MA/CS	1043:1054	arg1	membranes					1056:1064	PHBV-g-MA/CS membranes	1043:1064	PHBV-g-MA/CS membranes	1043:1064	Cell-cycle and apoptosis assays by FBs on the PHBV-series membrane samples were not affected by DNA content related to damage; i.e. rapid apoptosis/necrosis was not observed, demonstrating the potential of PHBV/CS or PHBV-g-MA/CS membranes for biomedical material applications.
26428145	5	31	theme	collagen	690:697	arg1	production					699:708	collagen production	690:708	collagen production	690:708	However, FB proliferation, collagen production and the percentage of normal cells growing on PHBV/CS membranes were greater than those for PHBV-g-MA/CS membranes.
26428145	1	32	theme	antibacterial	129:141	arg1	activity					143:150	The antibacterial activity	125:150	The antibacterial activity	125:150	The antibacterial activity and biocompatibility of membranes of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and chitosan (CS) (PHBV)/CS) were evaluated in this study.
26428145	6	33	theme	PHBV/CS	1032:1038	arg1	potential					1019:1027	the potential	1015:1027	the potential of PHBV/CS or PHBV-g-MA/CS membranes for biomedical material applications	1015:1101	Cell-cycle and apoptosis assays by FBs on the PHBV-series membrane samples were not affected by DNA content related to damage; i.e. rapid apoptosis/necrosis was not observed, demonstrating the potential of PHBV/CS or PHBV-g-MA/CS membranes for biomedical material applications.
26428145	6	34	theme	membranes	1056:1064	arg1	potential					1019:1027	the potential	1015:1027	the potential of PHBV/CS or PHBV-g-MA/CS membranes for biomedical material applications	1015:1101	Cell-cycle and apoptosis assays by FBs on the PHBV-series membrane samples were not affected by DNA content related to damage; i.e. rapid apoptosis/necrosis was not observed, demonstrating the potential of PHBV/CS or PHBV-g-MA/CS membranes for biomedical material applications.
26428145	1	35	theme	chitosan	245:252	arg1	/CS					265:267	poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and chitosan (CS) (PHBV)/CS)	189:268	poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and chitosan (CS) (PHBV)/CS)	189:268	The antibacterial activity and biocompatibility of membranes of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and chitosan (CS) (PHBV)/CS) were evaluated in this study.
26428145	3	36	theme	Mouse	404:408	arg1	fibroblasts					420:430	Mouse tail skin fibroblasts	404:430	Mouse tail skin fibroblasts (FBs)	404:436	Mouse tail skin fibroblasts (FBs) were seeded on two series of these films to assess cytocompatibility.
26428145	3	36	theme	Mouse	404:408	arg1	FBs					433:435	FBs	433:435	FBs	433:435	Mouse tail skin fibroblasts (FBs) were seeded on two series of these films to assess cytocompatibility.
26428145	5	37	theme	PHBV-g-MA/CS	802:813	arg1	membranes					815:823	PHBV-g-MA/CS membranes	802:823	PHBV-g-MA/CS membranes	802:823	However, FB proliferation, collagen production and the percentage of normal cells growing on PHBV/CS membranes were greater than those for PHBV-g-MA/CS membranes.
26428145	6	38	theme	DNA	922:924	arg1	content					926:932	DNA content	922:932	DNA content related to damage	922:950	Cell-cycle and apoptosis assays by FBs on the PHBV-series membrane samples were not affected by DNA content related to damage; i.e. rapid apoptosis/necrosis was not observed, demonstrating the potential of PHBV/CS or PHBV-g-MA/CS membranes for biomedical material applications.
26428145	7	39	theme	Escherichia	1151:1161	arg1	BCRC					1169:1172	BCRC 10239	1169:1178	BCRC 10239	1169:1178	Moreover, CS-based polysaccharide enhanced the Escherichia coli (BCRC 10239) antibacterial activity of the membranes.
26428145	7	39	theme	Escherichia	1151:1161	arg1	activity					1195:1202	the Escherichia coli (BCRC 10239) antibacterial activity	1147:1202	the Escherichia coli (BCRC 10239) antibacterial activity of the membranes	1147:1219	Moreover, CS-based polysaccharide enhanced the Escherichia coli (BCRC 10239) antibacterial activity of the membranes.
26428145	6	40	theme	related	934:940	arg1	content					926:932	DNA content	922:932	DNA content related to damage	922:950	Cell-cycle and apoptosis assays by FBs on the PHBV-series membrane samples were not affected by DNA content related to damage; i.e. rapid apoptosis/necrosis was not observed, demonstrating the potential of PHBV/CS or PHBV-g-MA/CS membranes for biomedical material applications.
26428145	6	41	theme	i.e.	953:956	arg1	apoptosis/necrosis					964:981	i.e. rapid apoptosis/necrosis	953:981	i.e. rapid apoptosis/necrosis	953:981	Cell-cycle and apoptosis assays by FBs on the PHBV-series membrane samples were not affected by DNA content related to damage; i.e. rapid apoptosis/necrosis was not observed, demonstrating the potential of PHBV/CS or PHBV-g-MA/CS membranes for biomedical material applications.
26428145	8	42	theme	PHBV-g-MA	1235:1243	arg1	Membranes					1222:1230	Membranes	1222:1230	Membranes of PHBV-g-MA or PHBV containing CS-based polysaccharide	1222:1286	Membranes of PHBV-g-MA or PHBV containing CS-based polysaccharide had better antibacterial activity.
26428145	6	43	theme	apoptosis	841:849	arg1	assays					851:856	apoptosis assays	841:856	apoptosis assays by FBs on the PHBV-series membrane	841:891	Cell-cycle and apoptosis assays by FBs on the PHBV-series membrane samples were not affected by DNA content related to damage; i.e. rapid apoptosis/necrosis was not observed, demonstrating the potential of PHBV/CS or PHBV-g-MA/CS membranes for biomedical material applications.
26428145	2	44	theme	-grafted	321:328	arg1	PHBV-g-MA					352:360	PHBV-g-MA	352:360	PHBV-g-MA	352:360	Maleic anhydride (MA)-grafted polyhydroxyalkanoate (PHBV-g-MA) was evaluated as an alternative to PHBV.
26428145	2	44	theme	-grafted	321:328	arg1	polyhydroxyalkanoate					330:349	Maleic anhydride (MA)-grafted polyhydroxyalkanoate	300:349	Maleic anhydride (MA)-grafted polyhydroxyalkanoate (PHBV-g-MA)	300:361	Maleic anhydride (MA)-grafted polyhydroxyalkanoate (PHBV-g-MA) was evaluated as an alternative to PHBV.
26428145	2	44	theme	-grafted	321:328	arg1	alternative					383:393	an alternative	380:393	an alternative to PHBV	380:401	Maleic anhydride (MA)-grafted polyhydroxyalkanoate (PHBV-g-MA) was evaluated as an alternative to PHBV.
26428145	4	45	theme	Collagen	508:515	arg1	analyses					540:547	Collagen and cell proliferation analyses	508:547	Collagen and cell proliferation analyses	508:547	Collagen and cell proliferation analyses indicated that PHBV, PHBV-g-MA and their composite membranes were biocompatible with respect to FB proliferation.
26428145	5	46	theme	FB	672:673	arg1	proliferation					675:687	FB proliferation	672:687	FB proliferation	672:687	However, FB proliferation, collagen production and the percentage of normal cells growing on PHBV/CS membranes were greater than those for PHBV-g-MA/CS membranes.
26428145	0	47	dep	in	27:28	arg1	vitro					30:34	vitro	30:34	vitro	30:34	Antibacterial activity and in vitro evaluation of the biocompatibility of chitosan-based polysaccharide/polyester membranes.
26428145	6	48	theme	Cell-cycle	826:835	arg1	samples					893:899	Cell-cycle and apoptosis assays by FBs on the PHBV-series membrane samples	826:899	Cell-cycle and apoptosis assays by FBs on the PHBV-series membrane samples	826:899	Cell-cycle and apoptosis assays by FBs on the PHBV-series membrane samples were not affected by DNA content related to damage; i.e. rapid apoptosis/necrosis was not observed, demonstrating the potential of PHBV/CS or PHBV-g-MA/CS membranes for biomedical material applications.
26428145	5	49	theme	cells	739:743	arg1	greater					779:785	greater	779:785	greater	779:785	However, FB proliferation, collagen production and the percentage of normal cells growing on PHBV/CS membranes were greater than those for PHBV-g-MA/CS membranes.
26428145	5	49	theme	cells	739:743	arg1	proliferation					675:687	FB proliferation	672:687	FB proliferation	672:687	However, FB proliferation, collagen production and the percentage of normal cells growing on PHBV/CS membranes were greater than those for PHBV-g-MA/CS membranes.
26428145	5	49	theme	cells	739:743	arg1	production					699:708	collagen production	690:708	collagen production	690:708	However, FB proliferation, collagen production and the percentage of normal cells growing on PHBV/CS membranes were greater than those for PHBV-g-MA/CS membranes.
26428145	5	49	theme	cells	739:743	arg1	percentage					718:727	the percentage	714:727	the percentage of normal cells growing on PHBV/CS membranes	714:772	However, FB proliferation, collagen production and the percentage of normal cells growing on PHBV/CS membranes were greater than those for PHBV-g-MA/CS membranes.
26428145	8	50	theme	antibacterial	1299:1311	arg1	activity					1313:1320	better antibacterial activity	1292:1320	better antibacterial activity	1292:1320	Membranes of PHBV-g-MA or PHBV containing CS-based polysaccharide had better antibacterial activity.
26428145	8	51	theme	better	1292:1297	arg1	activity					1313:1320	better antibacterial activity	1292:1320	better antibacterial activity	1292:1320	Membranes of PHBV-g-MA or PHBV containing CS-based polysaccharide had better antibacterial activity.
26428145	1	52	theme	membranes	176:184	arg1	biocompatibility					156:171	biocompatibility	156:171	biocompatibility of membranes of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and chitosan (CS) (PHBV)/CS)	156:268	The antibacterial activity and biocompatibility of membranes of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and chitosan (CS) (PHBV)/CS) were evaluated in this study.
26428145	1	52	theme	membranes	176:184	arg1	activity					143:150	The antibacterial activity	125:150	The antibacterial activity	125:150	The antibacterial activity and biocompatibility of membranes of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and chitosan (CS) (PHBV)/CS) were evaluated in this study.
26428145	8	53	theme	PHBV	1248:1251	arg1	Membranes					1222:1230	Membranes	1222:1230	Membranes of PHBV-g-MA or PHBV containing CS-based polysaccharide	1222:1286	Membranes of PHBV-g-MA or PHBV containing CS-based polysaccharide had better antibacterial activity.
26428145	0	54	theme	biocompatibility	54:69	arg1	evaluation					36:45	in vitro evaluation	27:45	in vitro evaluation of the biocompatibility of chitosan-based polysaccharide/polyester membranes	27:122	Antibacterial activity and in vitro evaluation of the biocompatibility of chitosan-based polysaccharide/polyester membranes.
26428145	0	54	theme	biocompatibility	54:69	arg1	activity					14:21	Antibacterial activity	0:21	Antibacterial activity	0:21	Antibacterial activity and in vitro evaluation of the biocompatibility of chitosan-based polysaccharide/polyester membranes.
26428145	4	55	theme	composite	590:598	arg1	membranes					600:608	their composite membranes	584:608	their composite membranes	584:608	Collagen and cell proliferation analyses indicated that PHBV, PHBV-g-MA and their composite membranes were biocompatible with respect to FB proliferation.
24285223	0	0	theme	fucoidan	91:98	arg1	effects					62:68	the pro- and anticoagulant effects	35:68	the pro- and anticoagulant effects of Fucus vesiculosus fucoidan	35:98	Structure-activity relationship of the pro- and anticoagulant effects of Fucus vesiculosus fucoidan.
24285223	9	1	theme	haemostasis	1497:1507	arg1	improvement					1482:1492	improvement	1482:1492	improvement of haemostasis in factor VIII/factor IX-deficient plasma	1482:1549	Fucoidan requires a minimal charge density of 0.5 sulfates per sugar unit and a size of 70 sugar units to demonstrate desired procoagulant activities for improvement of haemostasis in factor VIII/factor IX-deficient plasma.
24285223	1	2	theme	brown	178:182	arg1	seaweed					184:190	brown seaweed	178:190	brown seaweed	178:190	Fucoidan is a highly complex sulfated polysaccharide commonly extracted from brown seaweed.
24285223	6	3	theme	time	999:1002	arg1	assays					1010:1015	calibrated automated thrombography (CAT) and activated partial thromboplastin time(aPTT) assays	921:1015	calibrated automated thrombography (CAT) and activated partial thromboplastin time(aPTT) assays	921:1015	The fractions' pro- and anticoagulant activities were assessed by calibrated automated thrombography (CAT) and activated partial thromboplastin time(aPTT) assays.
24285223	3	4	theme	molecular	386:394	arg1	Mw					404:405	Mw	404:405	Mw	404:405	Their structural features, i.e. molecular weight (Mw), Mw distribution, degree of sulfation, monosaccharide composition, and different linkages, are known to affect these activities.
24285223	3	4	theme	molecular	386:394	arg1	weight					396:401	molecular weight	386:401	molecular weight (Mw)	386:406	Their structural features, i.e. molecular weight (Mw), Mw distribution, degree of sulfation, monosaccharide composition, and different linkages, are known to affect these activities.
24285223	9	5	from	improvement	1482:1492	arg1	plasma					1544:1549	factor VIII/factor IX-deficient plasma	1512:1549	factor VIII/factor IX-deficient plasma	1512:1549	Fucoidan requires a minimal charge density of 0.5 sulfates per sugar unit and a size of 70 sugar units to demonstrate desired procoagulant activities for improvement of haemostasis in factor VIII/factor IX-deficient plasma.
24285223	9	6	theme	factor	1512:1517	arg1	plasma					1544:1549	factor VIII/factor IX-deficient plasma	1512:1549	factor VIII/factor IX-deficient plasma	1512:1549	Fucoidan requires a minimal charge density of 0.5 sulfates per sugar unit and a size of 70 sugar units to demonstrate desired procoagulant activities for improvement of haemostasis in factor VIII/factor IX-deficient plasma.
24285223	6	7	theme	thromboplastin	984:997	arg1	time					999:1002	activated partial thromboplastin time	966:1002	activated partial thromboplastin time(aPTT)	966:1008	The fractions' pro- and anticoagulant activities were assessed by calibrated automated thrombography (CAT) and activated partial thromboplastin time(aPTT) assays.
24285223	6	7	theme	thromboplastin	984:997	arg1	aPTT					1004:1007	aPTT	1004:1007	aPTT	1004:1007	The fractions' pro- and anticoagulant activities were assessed by calibrated automated thrombography (CAT) and activated partial thromboplastin time(aPTT) assays.
24285223	6	8	theme	anticoagulant	879:891	arg1	activities					893:902	pro- and anticoagulant activities	870:902	pro- and anticoagulant activities	870:902	The fractions' pro- and anticoagulant activities were assessed by calibrated automated thrombography (CAT) and activated partial thromboplastin time(aPTT) assays.
24285223	9	9	theme	VIII/factor	1519:1529	arg1	plasma					1544:1549	factor VIII/factor IX-deficient plasma	1512:1549	factor VIII/factor IX-deficient plasma	1512:1549	Fucoidan requires a minimal charge density of 0.5 sulfates per sugar unit and a size of 70 sugar units to demonstrate desired procoagulant activities for improvement of haemostasis in factor VIII/factor IX-deficient plasma.
24285223	6	10	theme	automated	932:940	arg1	thrombography					942:954	calibrated automated thrombography	921:954	calibrated automated thrombography (CAT)	921:960	The fractions' pro- and anticoagulant activities were assessed by calibrated automated thrombography (CAT) and activated partial thromboplastin time(aPTT) assays.
24285223	6	10	theme	automated	932:940	arg1	CAT					957:959	CAT	957:959	CAT	957:959	The fractions' pro- and anticoagulant activities were assessed by calibrated automated thrombography (CAT) and activated partial thromboplastin time(aPTT) assays.
24285223	8	11	theme	anticoagulant	1302:1314	arg1	activities					1316:1325	pro- and anticoagulant activities	1293:1325	fucoidan's pro- and anticoagulant activities	1282:1325	This paper discusses the impact of charge density, size, and sugar composition on fucoidan's pro- and anticoagulant activities.
24285223	4	12	theme	fucoidan	598:605	arg1	analysis					586:593	structure-activity relationship (SAR) analysis	548:593	structure-activity relationship (SAR) analysis of fucoidan	548:605	Therefore, structure-activity relationship (SAR) analysis of fucoidan is crucial for its potential use as a procoagulant.
24285223	9	13	theme	desired	1446:1452	arg1	activities					1467:1476	desired procoagulant activities	1446:1476	desired procoagulant activities for improvement of haemostasis in factor VIII/factor IX-deficient plasma	1446:1549	Fucoidan requires a minimal charge density of 0.5 sulfates per sugar unit and a size of 70 sugar units to demonstrate desired procoagulant activities for improvement of haemostasis in factor VIII/factor IX-deficient plasma.
24285223	7	14	theme	pathway	1087:1093	arg1	TFPI					1106:1109	TFPI	1106:1109	TFPI	1106:1109	Binding to and inhibition of the anticoagulant protein tissue factor pathway inhibitor (TFPI) and the ability to activate coagulation via the contact pathway were also investigated.
24285223	7	14	theme	pathway	1087:1093	arg1	inhibitor					1095:1103	the anticoagulant protein tissue factor pathway inhibitor	1047:1103	the anticoagulant protein tissue factor pathway inhibitor (TFPI)	1047:1110	Binding to and inhibition of the anticoagulant protein tissue factor pathway inhibitor (TFPI) and the ability to activate coagulation via the contact pathway were also investigated.
24285223	8	15	theme	density	1242:1248	arg1	impact					1225:1230	the impact	1221:1230	the impact of charge density, size, and sugar composition on fucoidan's pro- and anticoagulant activities	1221:1325	This paper discusses the impact of charge density, size, and sugar composition on fucoidan's pro- and anticoagulant activities.
24285223	9	16	theme	IX-deficient	1531:1542	arg1	plasma					1544:1549	factor VIII/factor IX-deficient plasma	1512:1549	factor VIII/factor IX-deficient plasma	1512:1549	Fucoidan requires a minimal charge density of 0.5 sulfates per sugar unit and a size of 70 sugar units to demonstrate desired procoagulant activities for improvement of haemostasis in factor VIII/factor IX-deficient plasma.
24285223	6	17	theme	partial	976:982	arg1	time					999:1002	activated partial thromboplastin time	966:1002	activated partial thromboplastin time(aPTT)	966:1008	The fractions' pro- and anticoagulant activities were assessed by calibrated automated thrombography (CAT) and activated partial thromboplastin time(aPTT) assays.
24285223	6	17	theme	partial	976:982	arg1	aPTT					1004:1007	aPTT	1004:1007	aPTT	1004:1007	The fractions' pro- and anticoagulant activities were assessed by calibrated automated thrombography (CAT) and activated partial thromboplastin time(aPTT) assays.
24285223	6	18	theme	pro-	870:873	arg1	activities					893:902	pro- and anticoagulant activities	870:902	pro- and anticoagulant activities	870:902	The fractions' pro- and anticoagulant activities were assessed by calibrated automated thrombography (CAT) and activated partial thromboplastin time(aPTT) assays.
24285223	7	19	theme	inhibitor	1095:1103	arg1	inhibition					1033:1042	inhibition	1033:1042	inhibition of the anticoagulant protein tissue factor pathway inhibitor (TFPI)	1033:1110	Binding to and inhibition of the anticoagulant protein tissue factor pathway inhibitor (TFPI) and the ability to activate coagulation via the contact pathway were also investigated.
24285223	7	19	theme	inhibitor	1095:1103	arg1	ability					1120:1126	the ability	1116:1126	the ability to activate coagulation via the contact pathway	1116:1174	Binding to and inhibition of the anticoagulant protein tissue factor pathway inhibitor (TFPI) and the ability to activate coagulation via the contact pathway were also investigated.
24285223	7	20	theme	contact	1160:1166	arg1	pathway					1168:1174	the contact pathway	1156:1174	the contact pathway	1156:1174	Binding to and inhibition of the anticoagulant protein tissue factor pathway inhibitor (TFPI) and the ability to activate coagulation via the contact pathway were also investigated.
24285223	3	21	theme	monosaccharide	447:460	arg1	composition					462:472	monosaccharide composition	447:472	monosaccharide composition	447:472	Their structural features, i.e. molecular weight (Mw), Mw distribution, degree of sulfation, monosaccharide composition, and different linkages, are known to affect these activities.
24285223	5	22	theme	different	779:787	arg1	degrees					789:795	different degrees	779:795	different degrees	779:795	In this study, Fucus vesiculosus (F.v.) fucoidan was fractionated by charge and size as well as over- and desulfated to different degrees to yield preparations with various structural properties.
24285223	7	23	theme	tissue	1073:1078	arg1	TFPI					1106:1109	TFPI	1106:1109	TFPI	1106:1109	Binding to and inhibition of the anticoagulant protein tissue factor pathway inhibitor (TFPI) and the ability to activate coagulation via the contact pathway were also investigated.
24285223	7	23	theme	tissue	1073:1078	arg1	inhibitor					1095:1103	the anticoagulant protein tissue factor pathway inhibitor	1047:1103	the anticoagulant protein tissue factor pathway inhibitor (TFPI)	1047:1110	Binding to and inhibition of the anticoagulant protein tissue factor pathway inhibitor (TFPI) and the ability to activate coagulation via the contact pathway were also investigated.
24285223	8	24	theme	charge	1235:1240	arg1	density					1242:1248	charge density	1235:1248	charge density	1235:1248	This paper discusses the impact of charge density, size, and sugar composition on fucoidan's pro- and anticoagulant activities.
24285223	0	25	theme	Structure-activity	0:17	arg1	relationship					19:30	Structure-activity relationship	0:30	Structure-activity relationship of the pro- and anticoagulant effects of Fucus vesiculosus fucoidan	0:98	Structure-activity relationship of the pro- and anticoagulant effects of Fucus vesiculosus fucoidan.
24285223	6	26	theme	activated	966:974	arg1	time					999:1002	activated partial thromboplastin time	966:1002	activated partial thromboplastin time(aPTT)	966:1008	The fractions' pro- and anticoagulant activities were assessed by calibrated automated thrombography (CAT) and activated partial thromboplastin time(aPTT) assays.
24285223	6	26	theme	activated	966:974	arg1	aPTT					1004:1007	aPTT	1004:1007	aPTT	1004:1007	The fractions' pro- and anticoagulant activities were assessed by calibrated automated thrombography (CAT) and activated partial thromboplastin time(aPTT) assays.
24285223	7	27	theme	factor	1080:1085	arg1	TFPI					1106:1109	TFPI	1106:1109	TFPI	1106:1109	Binding to and inhibition of the anticoagulant protein tissue factor pathway inhibitor (TFPI) and the ability to activate coagulation via the contact pathway were also investigated.
24285223	7	27	theme	factor	1080:1085	arg1	inhibitor					1095:1103	the anticoagulant protein tissue factor pathway inhibitor	1047:1103	the anticoagulant protein tissue factor pathway inhibitor (TFPI)	1047:1110	Binding to and inhibition of the anticoagulant protein tissue factor pathway inhibitor (TFPI) and the ability to activate coagulation via the contact pathway were also investigated.
24285223	9	28	theme	minimal	1348:1354	arg1	density					1363:1369	a minimal charge density	1346:1369	a minimal charge density of 0.5 sulfates per sugar unit	1346:1400	Fucoidan requires a minimal charge density of 0.5 sulfates per sugar unit and a size of 70 sugar units to demonstrate desired procoagulant activities for improvement of haemostasis in factor VIII/factor IX-deficient plasma.
24285223	2	29	theme	concentration	332:344	arg1	ranges					346:351	different concentration ranges	322:351	different concentration ranges	322:351	In addition to their many biological activities, fucoidans have recently been demonstrated to inhibit or increase coagulation at different concentration ranges.
24285223	3	30	theme	sulfation	436:444	arg1	linkages					489:496	different linkages	479:496	different linkages	479:496	Their structural features, i.e. molecular weight (Mw), Mw distribution, degree of sulfation, monosaccharide composition, and different linkages, are known to affect these activities.
24285223	3	30	theme	sulfation	436:444	arg1	weight					396:401	molecular weight	386:401	molecular weight (Mw)	386:406	Their structural features, i.e. molecular weight (Mw), Mw distribution, degree of sulfation, monosaccharide composition, and different linkages, are known to affect these activities.
24285223	3	30	theme	sulfation	436:444	arg1	distribution					412:423	Mw distribution	409:423	Mw distribution	409:423	Their structural features, i.e. molecular weight (Mw), Mw distribution, degree of sulfation, monosaccharide composition, and different linkages, are known to affect these activities.
24285223	3	30	theme	sulfation	436:444	arg1	composition					462:472	monosaccharide composition	447:472	monosaccharide composition	447:472	Their structural features, i.e. molecular weight (Mw), Mw distribution, degree of sulfation, monosaccharide composition, and different linkages, are known to affect these activities.
24285223	3	30	theme	sulfation	436:444	arg1	degree					426:431	degree	426:431	degree of sulfation	426:444	Their structural features, i.e. molecular weight (Mw), Mw distribution, degree of sulfation, monosaccharide composition, and different linkages, are known to affect these activities.
24285223	3	30	theme	sulfation	436:444	arg1	Mw					404:405	Mw	404:405	Mw	404:405	Their structural features, i.e. molecular weight (Mw), Mw distribution, degree of sulfation, monosaccharide composition, and different linkages, are known to affect these activities.
24285223	4	31	theme	relationship	567:578	arg1	analysis					586:593	structure-activity relationship (SAR) analysis	548:593	structure-activity relationship (SAR) analysis of fucoidan	548:605	Therefore, structure-activity relationship (SAR) analysis of fucoidan is crucial for its potential use as a procoagulant.
24285223	7	32	theme	anticoagulant	1051:1063	arg1	TFPI					1106:1109	TFPI	1106:1109	TFPI	1106:1109	Binding to and inhibition of the anticoagulant protein tissue factor pathway inhibitor (TFPI) and the ability to activate coagulation via the contact pathway were also investigated.
24285223	7	32	theme	anticoagulant	1051:1063	arg1	inhibitor					1095:1103	the anticoagulant protein tissue factor pathway inhibitor	1047:1103	the anticoagulant protein tissue factor pathway inhibitor (TFPI)	1047:1110	Binding to and inhibition of the anticoagulant protein tissue factor pathway inhibitor (TFPI) and the ability to activate coagulation via the contact pathway were also investigated.
24285223	2	33	theme	different	322:330	arg1	ranges					346:351	different concentration ranges	322:351	different concentration ranges	322:351	In addition to their many biological activities, fucoidans have recently been demonstrated to inhibit or increase coagulation at different concentration ranges.
24285223	9	34	theme	sugar	1419:1423	arg1	units					1425:1429	70 sugar units	1416:1429	70 sugar units	1416:1429	Fucoidan requires a minimal charge density of 0.5 sulfates per sugar unit and a size of 70 sugar units to demonstrate desired procoagulant activities for improvement of haemostasis in factor VIII/factor IX-deficient plasma.
24285223	7	35	theme	protein	1065:1071	arg1	TFPI					1106:1109	TFPI	1106:1109	TFPI	1106:1109	Binding to and inhibition of the anticoagulant protein tissue factor pathway inhibitor (TFPI) and the ability to activate coagulation via the contact pathway were also investigated.
24285223	7	35	theme	protein	1065:1071	arg1	inhibitor					1095:1103	the anticoagulant protein tissue factor pathway inhibitor	1047:1103	the anticoagulant protein tissue factor pathway inhibitor (TFPI)	1047:1110	Binding to and inhibition of the anticoagulant protein tissue factor pathway inhibitor (TFPI) and the ability to activate coagulation via the contact pathway were also investigated.
24285223	8	36	theme	pro-	1293:1296	arg1	activities					1316:1325	pro- and anticoagulant activities	1293:1325	fucoidan's pro- and anticoagulant activities	1282:1325	This paper discusses the impact of charge density, size, and sugar composition on fucoidan's pro- and anticoagulant activities.
24285223	3	37	theme	different	479:487	arg1	linkages					489:496	different linkages	479:496	different linkages	479:496	Their structural features, i.e. molecular weight (Mw), Mw distribution, degree of sulfation, monosaccharide composition, and different linkages, are known to affect these activities.
24285223	9	38	theme	procoagulant	1454:1465	arg1	activities					1467:1476	desired procoagulant activities	1446:1476	desired procoagulant activities for improvement of haemostasis in factor VIII/factor IX-deficient plasma	1446:1549	Fucoidan requires a minimal charge density of 0.5 sulfates per sugar unit and a size of 70 sugar units to demonstrate desired procoagulant activities for improvement of haemostasis in factor VIII/factor IX-deficient plasma.
24285223	5	39	with	preparations	806:817	arg1	properties					843:852	various structural properties	824:852	various structural properties	824:852	In this study, Fucus vesiculosus (F.v.) fucoidan was fractionated by charge and size as well as over- and desulfated to different degrees to yield preparations with various structural properties.
24285223	5	40	theme	various	824:830	arg1	properties					843:852	various structural properties	824:852	various structural properties	824:852	In this study, Fucus vesiculosus (F.v.) fucoidan was fractionated by charge and size as well as over- and desulfated to different degrees to yield preparations with various structural properties.
24285223	0	41	theme	pro-	39:42	arg1	effects					62:68	the pro- and anticoagulant effects	35:68	the pro- and anticoagulant effects of Fucus vesiculosus fucoidan	35:98	Structure-activity relationship of the pro- and anticoagulant effects of Fucus vesiculosus fucoidan.
24285223	9	42	theme	charge	1356:1361	arg1	density					1363:1369	a minimal charge density	1346:1369	a minimal charge density of 0.5 sulfates per sugar unit	1346:1400	Fucoidan requires a minimal charge density of 0.5 sulfates per sugar unit and a size of 70 sugar units to demonstrate desired procoagulant activities for improvement of haemostasis in factor VIII/factor IX-deficient plasma.
24285223	5	43	theme	structural	832:841	arg1	properties					843:852	various structural properties	824:852	various structural properties	824:852	In this study, Fucus vesiculosus (F.v.) fucoidan was fractionated by charge and size as well as over- and desulfated to different degrees to yield preparations with various structural properties.
24285223	0	44	theme	effects	62:68	arg1	relationship					19:30	Structure-activity relationship	0:30	Structure-activity relationship of the pro- and anticoagulant effects of Fucus vesiculosus fucoidan	0:98	Structure-activity relationship of the pro- and anticoagulant effects of Fucus vesiculosus fucoidan.
24285223	5	45	dep	vesiculosus	680:690	arg1	F.v.					693:696	F.v.	693:696	F.v.	693:696	In this study, Fucus vesiculosus (F.v.) fucoidan was fractionated by charge and size as well as over- and desulfated to different degrees to yield preparations with various structural properties.
24285223	8	46	theme	sugar	1261:1265	arg1	composition					1267:1277	sugar composition	1261:1277	sugar composition	1261:1277	This paper discusses the impact of charge density, size, and sugar composition on fucoidan's pro- and anticoagulant activities.
24285223	0	47	theme	anticoagulant	48:60	arg1	effects					62:68	the pro- and anticoagulant effects	35:68	the pro- and anticoagulant effects of Fucus vesiculosus fucoidan	35:98	Structure-activity relationship of the pro- and anticoagulant effects of Fucus vesiculosus fucoidan.
24285223	4	48	theme	structure-activity	548:565	arg1	SAR					581:583	SAR	581:583	SAR	581:583	Therefore, structure-activity relationship (SAR) analysis of fucoidan is crucial for its potential use as a procoagulant.
24285223	4	48	theme	structure-activity	548:565	arg1	relationship					567:578	structure-activity relationship	548:578	structure-activity relationship (SAR) analysis of fucoidan	548:605	Therefore, structure-activity relationship (SAR) analysis of fucoidan is crucial for its potential use as a procoagulant.
24285223	2	49	theme	biological	219:228	arg1	activities					230:239	their many biological activities	208:239	their many biological activities	208:239	In addition to their many biological activities, fucoidans have recently been demonstrated to inhibit or increase coagulation at different concentration ranges.
24285223	8	50	dep	fucoidan	1282:1289	arg1	activities					1316:1325	pro- and anticoagulant activities	1293:1325	fucoidan's pro- and anticoagulant activities	1282:1325	This paper discusses the impact of charge density, size, and sugar composition on fucoidan's pro- and anticoagulant activities.
24285223	8	51	from	impact	1225:1230	arg1	fucoidan					1282:1289	fucoidan	1282:1289	fucoidan's pro- and anticoagulant activities	1282:1325	This paper discusses the impact of charge density, size, and sugar composition on fucoidan's pro- and anticoagulant activities.
24285223	1	52	theme	complex	122:128	arg1	Fucoidan					101:108	Fucoidan	101:108	Fucoidan	101:108	Fucoidan is a highly complex sulfated polysaccharide commonly extracted from brown seaweed.
24285223	1	52	theme	complex	122:128	arg1	polysaccharide					139:152	a highly complex sulfated polysaccharide	113:152	a highly complex sulfated polysaccharide commonly extracted from brown seaweed	113:190	Fucoidan is a highly complex sulfated polysaccharide commonly extracted from brown seaweed.
24285223	6	53	theme	calibrated	921:930	arg1	thrombography					942:954	calibrated automated thrombography	921:954	calibrated automated thrombography (CAT)	921:960	The fractions' pro- and anticoagulant activities were assessed by calibrated automated thrombography (CAT) and activated partial thromboplastin time(aPTT) assays.
24285223	6	53	theme	calibrated	921:930	arg1	CAT					957:959	CAT	957:959	CAT	957:959	The fractions' pro- and anticoagulant activities were assessed by calibrated automated thrombography (CAT) and activated partial thromboplastin time(aPTT) assays.
24285223	2	54	theme	many	214:217	arg1	activities					230:239	their many biological activities	208:239	their many biological activities	208:239	In addition to their many biological activities, fucoidans have recently been demonstrated to inhibit or increase coagulation at different concentration ranges.
24285223	1	55	theme	sulfated	130:137	arg1	Fucoidan					101:108	Fucoidan	101:108	Fucoidan	101:108	Fucoidan is a highly complex sulfated polysaccharide commonly extracted from brown seaweed.
24285223	1	55	theme	sulfated	130:137	arg1	polysaccharide					139:152	a highly complex sulfated polysaccharide	113:152	a highly complex sulfated polysaccharide commonly extracted from brown seaweed	113:190	Fucoidan is a highly complex sulfated polysaccharide commonly extracted from brown seaweed.
24285223	9	56	theme	units	1425:1429	arg1	density					1363:1369	a minimal charge density	1346:1369	a minimal charge density of 0.5 sulfates per sugar unit	1346:1400	Fucoidan requires a minimal charge density of 0.5 sulfates per sugar unit and a size of 70 sugar units to demonstrate desired procoagulant activities for improvement of haemostasis in factor VIII/factor IX-deficient plasma.
24285223	9	56	theme	units	1425:1429	arg1	size					1408:1411	a size	1406:1411	a size of 70 sugar units	1406:1429	Fucoidan requires a minimal charge density of 0.5 sulfates per sugar unit and a size of 70 sugar units to demonstrate desired procoagulant activities for improvement of haemostasis in factor VIII/factor IX-deficient plasma.
24285223	3	57	dep	weight	396:401	arg1	i.e.					381:384	i.e.	381:384	i.e.	381:384	Their structural features, i.e. molecular weight (Mw), Mw distribution, degree of sulfation, monosaccharide composition, and different linkages, are known to affect these activities.
24285223	9	58	theme	sugar	1391:1395	arg1	unit					1397:1400	sugar unit	1391:1400	sugar unit	1391:1400	Fucoidan requires a minimal charge density of 0.5 sulfates per sugar unit and a size of 70 sugar units to demonstrate desired procoagulant activities for improvement of haemostasis in factor VIII/factor IX-deficient plasma.
24285223	8	59	theme	composition	1267:1277	arg1	impact					1225:1230	the impact	1221:1230	the impact of charge density, size, and sugar composition on fucoidan's pro- and anticoagulant activities	1221:1325	This paper discusses the impact of charge density, size, and sugar composition on fucoidan's pro- and anticoagulant activities.
24285223	3	60	theme	structural	360:369	arg1	features					371:378	Their structural features	354:378	Their structural features	354:378	Their structural features, i.e. molecular weight (Mw), Mw distribution, degree of sulfation, monosaccharide composition, and different linkages, are known to affect these activities.
24285223	8	61	theme	size	1251:1254	arg1	impact					1225:1230	the impact	1221:1230	the impact of charge density, size, and sugar composition on fucoidan's pro- and anticoagulant activities	1221:1325	This paper discusses the impact of charge density, size, and sugar composition on fucoidan's pro- and anticoagulant activities.
24285223	4	62	theme	potential	626:634	arg1	use					636:638	its potential use	622:638	its potential use as a procoagulant	622:656	Therefore, structure-activity relationship (SAR) analysis of fucoidan is crucial for its potential use as a procoagulant.
24285223	3	63	theme	Mw	409:410	arg1	distribution					412:423	Mw distribution	409:423	Mw distribution	409:423	Their structural features, i.e. molecular weight (Mw), Mw distribution, degree of sulfation, monosaccharide composition, and different linkages, are known to affect these activities.
24285223	9	64	theme	sulfates	1378:1385	arg1	density					1363:1369	a minimal charge density	1346:1369	a minimal charge density of 0.5 sulfates per sugar unit	1346:1400	Fucoidan requires a minimal charge density of 0.5 sulfates per sugar unit and a size of 70 sugar units to demonstrate desired procoagulant activities for improvement of haemostasis in factor VIII/factor IX-deficient plasma.
24285223	9	64	theme	sulfates	1378:1385	arg1	size					1408:1411	a size	1406:1411	a size of 70 sugar units	1406:1429	Fucoidan requires a minimal charge density of 0.5 sulfates per sugar unit and a size of 70 sugar units to demonstrate desired procoagulant activities for improvement of haemostasis in factor VIII/factor IX-deficient plasma.
24285223	6	65	theme	thrombography	942:954	arg1	assays					1010:1015	calibrated automated thrombography (CAT) and activated partial thromboplastin time(aPTT) assays	921:1015	calibrated automated thrombography (CAT) and activated partial thromboplastin time(aPTT) assays	921:1015	The fractions' pro- and anticoagulant activities were assessed by calibrated automated thrombography (CAT) and activated partial thromboplastin time(aPTT) assays.
27997408	2	0	theme	ethylenediaminetetraacetic	433:458	arg1	acid					460:463	ethylenediaminetetraacetic acid	433:463	ethylenediaminetetraacetic acid (EDTA)	433:470	To develop an effective but harmless method for EPS extraction from Shewanella oneidensis MR-1, five extraction methods, i.e. centrifugation (control), heating (40, 45, 50, and 60 °C), and treatments with H2SO4, ethylenediaminetetraacetic acid (EDTA) and NaOH, were examined, respectively.
27997408	2	0	theme	ethylenediaminetetraacetic	433:458	arg1	EDTA					466:469	EDTA	466:469	EDTA	466:469	To develop an effective but harmless method for EPS extraction from Shewanella oneidensis MR-1, five extraction methods, i.e. centrifugation (control), heating (40, 45, 50, and 60 °C), and treatments with H2SO4, ethylenediaminetetraacetic acid (EDTA) and NaOH, were examined, respectively.
27997408	3	1	theme	heating	647:653	arg1	temperature					655:665	heating temperature	647:665	heating temperature	647:665	Results from scanning electron microscope and flow cytometric analyses indicate that MR-1 cells were severely broken by H2SO4, NaOH and heating temperature ≥45 °C. Proteins and polysaccharides in EPS extracted by heating at 40 °C were 7.12 and 1.60 mg g-1 dry cell, respectively.
27997408	4	2	dep	cell	916:919	arg1	mg					905:906	mg	905:906	mg	905:906	Although EDTA treatment had a relatively lower yield of EPS (proteins and polysaccharides yields of 5.15 and 1.30 mg g-1 dry cell, respectively), cell lysis was barely found after EPS extraction.
27997408	1	3	theme	bio-aggregate	164:176	arg1	formation					178:186	bio-aggregate formation	164:186	bio-aggregate formation	164:186	Extracellular polymeric substances (EPS) play crucial roles in bio-aggregate formation and survival of bacterial cells.
27997408	4	4	theme	cell	937:940	arg1	lysis					942:946	cell lysis	937:946	cell lysis	937:946	Although EDTA treatment had a relatively lower yield of EPS (proteins and polysaccharides yields of 5.15 and 1.30 mg g-1 dry cell, respectively), cell lysis was barely found after EPS extraction.
27997408	3	5	theme	mg	760:761	arg1	g-1					763:765	7.12 and 1.60 mg g-1 dry cell	746:774	g-1	763:765	Results from scanning electron microscope and flow cytometric analyses indicate that MR-1 cells were severely broken by H2SO4, NaOH and heating temperature ≥45 °C. Proteins and polysaccharides in EPS extracted by heating at 40 °C were 7.12 and 1.60 mg g-1 dry cell, respectively.
27997408	7	6	theme	cell	1385:1388	arg1	lysis					1390:1394	the cell lysis and EPS content	1381:1410	lysis	1390:1394	In short, heating (40 °C) and EDTA treatments were found the most suitable methods for EPS extraction considering the cell lysis and EPS content, composition and functional groups together.
27997408	6	7	theme	good	1189:1192	arg1	accordance					1194:1203	good accordance	1189:1203	good accordance with protein concentration measured by the chemical analysis	1189:1264	Furthermore, the peak intensity was in good accordance with protein concentration measured by the chemical analysis.
27997408	4	8	dep	proteins	852:859	arg1	yields					881:886	yields	881:886	yields of 5.15 and 1.30 mg g-1 dry cell	881:919	Although EDTA treatment had a relatively lower yield of EPS (proteins and polysaccharides yields of 5.15 and 1.30 mg g-1 dry cell, respectively), cell lysis was barely found after EPS extraction.
27997408	3	9	theme	MR-1	596:599	arg1	cells					601:605	MR-1 cells	596:605	MR-1 cells	596:605	Results from scanning electron microscope and flow cytometric analyses indicate that MR-1 cells were severely broken by H2SO4, NaOH and heating temperature ≥45 °C. Proteins and polysaccharides in EPS extracted by heating at 40 °C were 7.12 and 1.60 mg g-1 dry cell, respectively.
27997408	5	10	theme	three-dimensional	1024:1040	arg1	spectrum					1069:1076	the three-dimensional excitation-emission matrix spectrum	1020:1076	the three-dimensional excitation-emission matrix spectrum of each EPS sample	1020:1095	Three peaks were identified from the three-dimensional excitation-emission matrix spectrum of each EPS sample, suggesting the presence of protein-like substances.
27997408	3	11	theme	scanning	524:531	arg1	microscope					542:551	scanning electron microscope	524:551	scanning electron microscope	524:551	Results from scanning electron microscope and flow cytometric analyses indicate that MR-1 cells were severely broken by H2SO4, NaOH and heating temperature ≥45 °C. Proteins and polysaccharides in EPS extracted by heating at 40 °C were 7.12 and 1.60 mg g-1 dry cell, respectively.
27997408	4	12	dep	EPS	847:849	arg1	polysaccharides					865:879	polysaccharides	865:879	polysaccharides	865:879	Although EDTA treatment had a relatively lower yield of EPS (proteins and polysaccharides yields of 5.15 and 1.30 mg g-1 dry cell, respectively), cell lysis was barely found after EPS extraction.
27997408	4	12	dep	EPS	847:849	arg1	proteins					852:859	proteins	852:859	proteins	852:859	Although EDTA treatment had a relatively lower yield of EPS (proteins and polysaccharides yields of 5.15 and 1.30 mg g-1 dry cell, respectively), cell lysis was barely found after EPS extraction.
27997408	3	13	theme	°C.	671:673	arg1	Proteins					675:682	≥45 °C. Proteins	667:682	≥45 °C. Proteins	667:682	Results from scanning electron microscope and flow cytometric analyses indicate that MR-1 cells were severely broken by H2SO4, NaOH and heating temperature ≥45 °C. Proteins and polysaccharides in EPS extracted by heating at 40 °C were 7.12 and 1.60 mg g-1 dry cell, respectively.
27997408	4	14	theme	EDTA	800:803	arg1	treatment					805:813	EDTA treatment	800:813	EDTA treatment	800:813	Although EDTA treatment had a relatively lower yield of EPS (proteins and polysaccharides yields of 5.15 and 1.30 mg g-1 dry cell, respectively), cell lysis was barely found after EPS extraction.
27997408	2	15	theme	oneidensis	300:309	arg1	MR-1					311:314	Shewanella oneidensis MR-1	289:314	Shewanella oneidensis MR-1	289:314	To develop an effective but harmless method for EPS extraction from Shewanella oneidensis MR-1, five extraction methods, i.e. centrifugation (control), heating (40, 45, 50, and 60 °C), and treatments with H2SO4, ethylenediaminetetraacetic acid (EDTA) and NaOH, were examined, respectively.
27997408	3	16	dep	H2SO4	631:635	arg1	Proteins					675:682	≥45 °C. Proteins	667:682	≥45 °C. Proteins	667:682	Results from scanning electron microscope and flow cytometric analyses indicate that MR-1 cells were severely broken by H2SO4, NaOH and heating temperature ≥45 °C. Proteins and polysaccharides in EPS extracted by heating at 40 °C were 7.12 and 1.60 mg g-1 dry cell, respectively.
27997408	6	17	with	accordance	1194:1203	arg1	concentration					1218:1230	protein concentration	1210:1230	protein concentration measured by the chemical analysis	1210:1264	Furthermore, the peak intensity was in good accordance with protein concentration measured by the chemical analysis.
27997408	6	18	theme	peak	1167:1170	arg1	intensity					1172:1180	the peak intensity	1163:1180	the peak intensity	1163:1180	Furthermore, the peak intensity was in good accordance with protein concentration measured by the chemical analysis.
27997408	2	19	theme	Shewanella	289:298	arg1	MR-1					311:314	Shewanella oneidensis MR-1	289:314	Shewanella oneidensis MR-1	289:314	To develop an effective but harmless method for EPS extraction from Shewanella oneidensis MR-1, five extraction methods, i.e. centrifugation (control), heating (40, 45, 50, and 60 °C), and treatments with H2SO4, ethylenediaminetetraacetic acid (EDTA) and NaOH, were examined, respectively.
27997408	3	20	theme	dry	767:769	arg1	cell					771:774	dry cell	767:774	dry cell	767:774	Results from scanning electron microscope and flow cytometric analyses indicate that MR-1 cells were severely broken by H2SO4, NaOH and heating temperature ≥45 °C. Proteins and polysaccharides in EPS extracted by heating at 40 °C were 7.12 and 1.60 mg g-1 dry cell, respectively.
27997408	7	21	dep	heating	1277:1283	arg1	treatments					1302:1311	treatments	1302:1311	treatments	1302:1311	In short, heating (40 °C) and EDTA treatments were found the most suitable methods for EPS extraction considering the cell lysis and EPS content, composition and functional groups together.
27997408	7	22	theme	suitable	1333:1340	arg1	methods					1342:1348	the most suitable methods	1324:1348	the most suitable methods for EPS extraction	1324:1367	In short, heating (40 °C) and EDTA treatments were found the most suitable methods for EPS extraction considering the cell lysis and EPS content, composition and functional groups together.
27997408	3	23	from	polysaccharides	688:702	arg1	EPS					707:709	EPS	707:709	EPS extracted by heating at 40 °C	707:739	Results from scanning electron microscope and flow cytometric analyses indicate that MR-1 cells were severely broken by H2SO4, NaOH and heating temperature ≥45 °C. Proteins and polysaccharides in EPS extracted by heating at 40 °C were 7.12 and 1.60 mg g-1 dry cell, respectively.
27997408	5	24	theme	substances	1138:1147	arg1	presence					1113:1120	the presence	1109:1120	the presence of protein-like substances	1109:1147	Three peaks were identified from the three-dimensional excitation-emission matrix spectrum of each EPS sample, suggesting the presence of protein-like substances.
27997408	3	25	from	analyses	573:580	arg1	Results					511:517	Results	511:517	Results from scanning electron microscope and flow cytometric analyses	511:580	Results from scanning electron microscope and flow cytometric analyses indicate that MR-1 cells were severely broken by H2SO4, NaOH and heating temperature ≥45 °C. Proteins and polysaccharides in EPS extracted by heating at 40 °C were 7.12 and 1.60 mg g-1 dry cell, respectively.
27997408	2	26	dep	method	258:263	arg1	examined					487:494	examined	487:494	examined	487:494	To develop an effective but harmless method for EPS extraction from Shewanella oneidensis MR-1, five extraction methods, i.e. centrifugation (control), heating (40, 45, 50, and 60 °C), and treatments with H2SO4, ethylenediaminetetraacetic acid (EDTA) and NaOH, were examined, respectively.
27997408	3	27	theme	≥45	667:669	arg1	Proteins					675:682	≥45 °C. Proteins	667:682	≥45 °C. Proteins	667:682	Results from scanning electron microscope and flow cytometric analyses indicate that MR-1 cells were severely broken by H2SO4, NaOH and heating temperature ≥45 °C. Proteins and polysaccharides in EPS extracted by heating at 40 °C were 7.12 and 1.60 mg g-1 dry cell, respectively.
27997408	1	28	theme	Extracellular	101:113	arg1	EPS					137:139	EPS	137:139	EPS	137:139	Extracellular polymeric substances (EPS) play crucial roles in bio-aggregate formation and survival of bacterial cells.
27997408	1	28	theme	Extracellular	101:113	arg1	substances					125:134	Extracellular polymeric substances	101:134	Extracellular polymeric substances (EPS)	101:140	Extracellular polymeric substances (EPS) play crucial roles in bio-aggregate formation and survival of bacterial cells.
27997408	7	29	theme	EPS	1354:1356	arg1	extraction					1358:1367	EPS extraction	1354:1367	EPS extraction	1354:1367	In short, heating (40 °C) and EDTA treatments were found the most suitable methods for EPS extraction considering the cell lysis and EPS content, composition and functional groups together.
27997408	0	30	theme	Effective	0:8	arg1	methods					10:16	Effective methods	0:16	Effective methods for extracting extracellular polymeric substances from Shewanella oneidensis MR-1.	0:99	Effective methods for extracting extracellular polymeric substances from Shewanella oneidensis MR-1.
27997408	2	31	theme	extraction	322:331	arg1	methods					333:339	five extraction methods	317:339	five extraction methods	317:339	To develop an effective but harmless method for EPS extraction from Shewanella oneidensis MR-1, five extraction methods, i.e. centrifugation (control), heating (40, 45, 50, and 60 °C), and treatments with H2SO4, ethylenediaminetetraacetic acid (EDTA) and NaOH, were examined, respectively.
27997408	1	32	theme	bacterial	204:212	arg1	cells					214:218	bacterial cells	204:218	bacterial cells	204:218	Extracellular polymeric substances (EPS) play crucial roles in bio-aggregate formation and survival of bacterial cells.
27997408	4	33	dep	mg	905:906	arg1	5.15					891:894	5.15	891:894	5.15	891:894	Although EDTA treatment had a relatively lower yield of EPS (proteins and polysaccharides yields of 5.15 and 1.30 mg g-1 dry cell, respectively), cell lysis was barely found after EPS extraction.
27997408	4	33	dep	mg	905:906	arg1	1.30					900:903	1.30	900:903	1.30	900:903	Although EDTA treatment had a relatively lower yield of EPS (proteins and polysaccharides yields of 5.15 and 1.30 mg g-1 dry cell, respectively), cell lysis was barely found after EPS extraction.
27997408	5	34	theme	EPS	1086:1088	arg1	sample					1090:1095	each EPS sample	1081:1095	each EPS sample	1081:1095	Three peaks were identified from the three-dimensional excitation-emission matrix spectrum of each EPS sample, suggesting the presence of protein-like substances.
27997408	1	35	theme	cells	214:218	arg1	survival					192:199	survival	192:199	survival	192:199	Extracellular polymeric substances (EPS) play crucial roles in bio-aggregate formation and survival of bacterial cells.
27997408	1	35	theme	cells	214:218	arg1	formation					178:186	bio-aggregate formation	164:186	bio-aggregate formation	164:186	Extracellular polymeric substances (EPS) play crucial roles in bio-aggregate formation and survival of bacterial cells.
27997408	1	36	theme	polymeric	115:123	arg1	EPS					137:139	EPS	137:139	EPS	137:139	Extracellular polymeric substances (EPS) play crucial roles in bio-aggregate formation and survival of bacterial cells.
27997408	1	36	theme	polymeric	115:123	arg1	substances					125:134	Extracellular polymeric substances	101:134	Extracellular polymeric substances (EPS)	101:140	Extracellular polymeric substances (EPS) play crucial roles in bio-aggregate formation and survival of bacterial cells.
27997408	5	37	theme	sample	1090:1095	arg1	spectrum					1069:1076	the three-dimensional excitation-emission matrix spectrum	1020:1076	the three-dimensional excitation-emission matrix spectrum of each EPS sample	1020:1095	Three peaks were identified from the three-dimensional excitation-emission matrix spectrum of each EPS sample, suggesting the presence of protein-like substances.
27997408	6	38	theme	chemical	1248:1255	arg1	analysis					1257:1264	the chemical analysis	1244:1264	the chemical analysis	1244:1264	Furthermore, the peak intensity was in good accordance with protein concentration measured by the chemical analysis.
27997408	2	39	dep	centrifugation	347:360	arg1	i.e.					342:345	i.e.	342:345	i.e.	342:345	To develop an effective but harmless method for EPS extraction from Shewanella oneidensis MR-1, five extraction methods, i.e. centrifugation (control), heating (40, 45, 50, and 60 °C), and treatments with H2SO4, ethylenediaminetetraacetic acid (EDTA) and NaOH, were examined, respectively.
27997408	3	40	from	microscope	542:551	arg1	Results					511:517	Results	511:517	Results from scanning electron microscope and flow cytometric analyses	511:580	Results from scanning electron microscope and flow cytometric analyses indicate that MR-1 cells were severely broken by H2SO4, NaOH and heating temperature ≥45 °C. Proteins and polysaccharides in EPS extracted by heating at 40 °C were 7.12 and 1.60 mg g-1 dry cell, respectively.
27997408	0	41	theme	polymeric	47:55	arg1	substances					57:66	extracellular polymeric substances	33:66	extracellular polymeric substances	33:66	Effective methods for extracting extracellular polymeric substances from Shewanella oneidensis MR-1.
27997408	7	42	theme	functional	1429:1438	arg1	groups					1440:1445	functional groups	1429:1445	functional groups	1429:1445	In short, heating (40 °C) and EDTA treatments were found the most suitable methods for EPS extraction considering the cell lysis and EPS content, composition and functional groups together.
27997408	2	43	theme	harmless	249:256	arg1	method					258:263	an effective but harmless method	232:263	an effective but harmless method for EPS extraction from Shewanella oneidensis MR-1, five extraction methods, i.e. centrifugation (control), heating (40, 45, 50, and 60 °C), and treatments with H2SO4, ethylenediaminetetraacetic acid (EDTA) and NaOH, were examined, respectively	232:508	To develop an effective but harmless method for EPS extraction from Shewanella oneidensis MR-1, five extraction methods, i.e. centrifugation (control), heating (40, 45, 50, and 60 °C), and treatments with H2SO4, ethylenediaminetetraacetic acid (EDTA) and NaOH, were examined, respectively.
27997408	7	44	theme	most	1328:1331	arg1	methods					1342:1348	the most suitable methods	1324:1348	the most suitable methods for EPS extraction	1324:1367	In short, heating (40 °C) and EDTA treatments were found the most suitable methods for EPS extraction considering the cell lysis and EPS content, composition and functional groups together.
27997408	0	45	theme	extracellular	33:45	arg1	substances					57:66	extracellular polymeric substances	33:66	extracellular polymeric substances	33:66	Effective methods for extracting extracellular polymeric substances from Shewanella oneidensis MR-1.
27997408	5	46	theme	excitation-emission	1042:1060	arg1	spectrum					1069:1076	the three-dimensional excitation-emission matrix spectrum	1020:1076	the three-dimensional excitation-emission matrix spectrum of each EPS sample	1020:1095	Three peaks were identified from the three-dimensional excitation-emission matrix spectrum of each EPS sample, suggesting the presence of protein-like substances.
27997408	4	47	theme	EPS	847:849	arg1	yield					838:842	a relatively lower yield	819:842	a relatively lower yield of EPS (proteins and polysaccharides yields of 5.15 and 1.30 mg g-1 dry cell, respectively)	819:934	Although EDTA treatment had a relatively lower yield of EPS (proteins and polysaccharides yields of 5.15 and 1.30 mg g-1 dry cell, respectively), cell lysis was barely found after EPS extraction.
27997408	4	48	theme	cell	916:919	arg1	yields					881:886	yields	881:886	yields of 5.15 and 1.30 mg g-1 dry cell	881:919	Although EDTA treatment had a relatively lower yield of EPS (proteins and polysaccharides yields of 5.15 and 1.30 mg g-1 dry cell, respectively), cell lysis was barely found after EPS extraction.
27997408	5	49	theme	matrix	1062:1067	arg1	spectrum					1069:1076	the three-dimensional excitation-emission matrix spectrum	1020:1076	the three-dimensional excitation-emission matrix spectrum of each EPS sample	1020:1095	Three peaks were identified from the three-dimensional excitation-emission matrix spectrum of each EPS sample, suggesting the presence of protein-like substances.
27997408	2	50	with	centrifugation	347:360	arg1	acid					460:463	ethylenediaminetetraacetic acid	433:463	ethylenediaminetetraacetic acid (EDTA)	433:470	To develop an effective but harmless method for EPS extraction from Shewanella oneidensis MR-1, five extraction methods, i.e. centrifugation (control), heating (40, 45, 50, and 60 °C), and treatments with H2SO4, ethylenediaminetetraacetic acid (EDTA) and NaOH, were examined, respectively.
27997408	2	50	with	centrifugation	347:360	arg1	H2SO4					426:430	H2SO4	426:430	H2SO4	426:430	To develop an effective but harmless method for EPS extraction from Shewanella oneidensis MR-1, five extraction methods, i.e. centrifugation (control), heating (40, 45, 50, and 60 °C), and treatments with H2SO4, ethylenediaminetetraacetic acid (EDTA) and NaOH, were examined, respectively.
27997408	2	50	with	centrifugation	347:360	arg1	EDTA					466:469	EDTA	466:469	EDTA	466:469	To develop an effective but harmless method for EPS extraction from Shewanella oneidensis MR-1, five extraction methods, i.e. centrifugation (control), heating (40, 45, 50, and 60 °C), and treatments with H2SO4, ethylenediaminetetraacetic acid (EDTA) and NaOH, were examined, respectively.
27997408	2	50	with	centrifugation	347:360	arg1	NaOH					476:479	NaOH	476:479	NaOH	476:479	To develop an effective but harmless method for EPS extraction from Shewanella oneidensis MR-1, five extraction methods, i.e. centrifugation (control), heating (40, 45, 50, and 60 °C), and treatments with H2SO4, ethylenediaminetetraacetic acid (EDTA) and NaOH, were examined, respectively.
27997408	2	51	theme	effective	235:243	arg1	method					258:263	an effective but harmless method	232:263	an effective but harmless method for EPS extraction from Shewanella oneidensis MR-1, five extraction methods, i.e. centrifugation (control), heating (40, 45, 50, and 60 °C), and treatments with H2SO4, ethylenediaminetetraacetic acid (EDTA) and NaOH, were examined, respectively	232:508	To develop an effective but harmless method for EPS extraction from Shewanella oneidensis MR-1, five extraction methods, i.e. centrifugation (control), heating (40, 45, 50, and 60 °C), and treatments with H2SO4, ethylenediaminetetraacetic acid (EDTA) and NaOH, were examined, respectively.
27997408	6	52	theme	protein	1210:1216	arg1	concentration					1218:1230	protein concentration	1210:1230	protein concentration measured by the chemical analysis	1210:1264	Furthermore, the peak intensity was in good accordance with protein concentration measured by the chemical analysis.
27997408	4	53	theme	dry	912:914	arg1	cell					916:919	5.15 and 1.30 mg g-1 dry cell	891:919	5.15 and 1.30 mg g-1 dry cell	891:919	Although EDTA treatment had a relatively lower yield of EPS (proteins and polysaccharides yields of 5.15 and 1.30 mg g-1 dry cell, respectively), cell lysis was barely found after EPS extraction.
27997408	7	54	located	found	1318:1322	arg1	short					1270:1274	short	1270:1274	short	1270:1274	In short, heating (40 °C) and EDTA treatments were found the most suitable methods for EPS extraction considering the cell lysis and EPS content, composition and functional groups together.
27997408	7	54	located	found	1318:1322	arg2	°C					1289:1290	40 °C	1286:1290	40 °C	1286:1290	In short, heating (40 °C) and EDTA treatments were found the most suitable methods for EPS extraction considering the cell lysis and EPS content, composition and functional groups together.
27997408	7	54	located	found	1318:1322	arg2	heating					1277:1283	heating	1277:1283	heating (40 °C)	1277:1291	In short, heating (40 °C) and EDTA treatments were found the most suitable methods for EPS extraction considering the cell lysis and EPS content, composition and functional groups together.
27997408	7	54	located	found	1318:1322	arg2	EDTA					1297:1300	EDTA	1297:1300	EDTA	1297:1300	In short, heating (40 °C) and EDTA treatments were found the most suitable methods for EPS extraction considering the cell lysis and EPS content, composition and functional groups together.
27997408	3	55	from	°C	738:739	arg1	heating					724:730	heating	724:730	heating at 40 °C	724:739	Results from scanning electron microscope and flow cytometric analyses indicate that MR-1 cells were severely broken by H2SO4, NaOH and heating temperature ≥45 °C. Proteins and polysaccharides in EPS extracted by heating at 40 °C were 7.12 and 1.60 mg g-1 dry cell, respectively.
27997408	7	56	theme	EPS	1400:1402	arg1	content					1404:1410	the cell lysis and EPS content	1381:1410	content	1404:1410	In short, heating (40 °C) and EDTA treatments were found the most suitable methods for EPS extraction considering the cell lysis and EPS content, composition and functional groups together.
27997408	3	57	theme	electron	533:540	arg1	microscope					542:551	scanning electron microscope	524:551	scanning electron microscope	524:551	Results from scanning electron microscope and flow cytometric analyses indicate that MR-1 cells were severely broken by H2SO4, NaOH and heating temperature ≥45 °C. Proteins and polysaccharides in EPS extracted by heating at 40 °C were 7.12 and 1.60 mg g-1 dry cell, respectively.
27997408	4	58	theme	lower	832:836	arg1	yield					838:842	a relatively lower yield	819:842	a relatively lower yield of EPS (proteins and polysaccharides yields of 5.15 and 1.30 mg g-1 dry cell, respectively)	819:934	Although EDTA treatment had a relatively lower yield of EPS (proteins and polysaccharides yields of 5.15 and 1.30 mg g-1 dry cell, respectively), cell lysis was barely found after EPS extraction.
27997408	5	59	theme	protein-like	1125:1136	arg1	substances					1138:1147	protein-like substances	1125:1147	protein-like substances	1125:1147	Three peaks were identified from the three-dimensional excitation-emission matrix spectrum of each EPS sample, suggesting the presence of protein-like substances.
27997408	4	60	theme	g-1	908:910	arg1	cell					916:919	5.15 and 1.30 mg g-1 dry cell	891:919	5.15 and 1.30 mg g-1 dry cell	891:919	Although EDTA treatment had a relatively lower yield of EPS (proteins and polysaccharides yields of 5.15 and 1.30 mg g-1 dry cell, respectively), cell lysis was barely found after EPS extraction.
27997408	2	61	with	heating	373:379	arg1	acid					460:463	ethylenediaminetetraacetic acid	433:463	ethylenediaminetetraacetic acid (EDTA)	433:470	To develop an effective but harmless method for EPS extraction from Shewanella oneidensis MR-1, five extraction methods, i.e. centrifugation (control), heating (40, 45, 50, and 60 °C), and treatments with H2SO4, ethylenediaminetetraacetic acid (EDTA) and NaOH, were examined, respectively.
27997408	2	61	with	heating	373:379	arg1	H2SO4					426:430	H2SO4	426:430	H2SO4	426:430	To develop an effective but harmless method for EPS extraction from Shewanella oneidensis MR-1, five extraction methods, i.e. centrifugation (control), heating (40, 45, 50, and 60 °C), and treatments with H2SO4, ethylenediaminetetraacetic acid (EDTA) and NaOH, were examined, respectively.
27997408	2	61	with	heating	373:379	arg1	EDTA					466:469	EDTA	466:469	EDTA	466:469	To develop an effective but harmless method for EPS extraction from Shewanella oneidensis MR-1, five extraction methods, i.e. centrifugation (control), heating (40, 45, 50, and 60 °C), and treatments with H2SO4, ethylenediaminetetraacetic acid (EDTA) and NaOH, were examined, respectively.
27997408	2	61	with	heating	373:379	arg1	NaOH					476:479	NaOH	476:479	NaOH	476:479	To develop an effective but harmless method for EPS extraction from Shewanella oneidensis MR-1, five extraction methods, i.e. centrifugation (control), heating (40, 45, 50, and 60 °C), and treatments with H2SO4, ethylenediaminetetraacetic acid (EDTA) and NaOH, were examined, respectively.
27997408	2	62	theme	EPS	269:271	arg1	extraction					273:282	EPS extraction	269:282	EPS extraction from Shewanella oneidensis MR-1	269:314	To develop an effective but harmless method for EPS extraction from Shewanella oneidensis MR-1, five extraction methods, i.e. centrifugation (control), heating (40, 45, 50, and 60 °C), and treatments with H2SO4, ethylenediaminetetraacetic acid (EDTA) and NaOH, were examined, respectively.
27997408	2	63	with	treatments	410:419	arg1	acid					460:463	ethylenediaminetetraacetic acid	433:463	ethylenediaminetetraacetic acid (EDTA)	433:470	To develop an effective but harmless method for EPS extraction from Shewanella oneidensis MR-1, five extraction methods, i.e. centrifugation (control), heating (40, 45, 50, and 60 °C), and treatments with H2SO4, ethylenediaminetetraacetic acid (EDTA) and NaOH, were examined, respectively.
27997408	2	63	with	treatments	410:419	arg1	H2SO4					426:430	H2SO4	426:430	H2SO4	426:430	To develop an effective but harmless method for EPS extraction from Shewanella oneidensis MR-1, five extraction methods, i.e. centrifugation (control), heating (40, 45, 50, and 60 °C), and treatments with H2SO4, ethylenediaminetetraacetic acid (EDTA) and NaOH, were examined, respectively.
27997408	2	63	with	treatments	410:419	arg1	EDTA					466:469	EDTA	466:469	EDTA	466:469	To develop an effective but harmless method for EPS extraction from Shewanella oneidensis MR-1, five extraction methods, i.e. centrifugation (control), heating (40, 45, 50, and 60 °C), and treatments with H2SO4, ethylenediaminetetraacetic acid (EDTA) and NaOH, were examined, respectively.
27997408	2	63	with	treatments	410:419	arg1	NaOH					476:479	NaOH	476:479	NaOH	476:479	To develop an effective but harmless method for EPS extraction from Shewanella oneidensis MR-1, five extraction methods, i.e. centrifugation (control), heating (40, 45, 50, and 60 °C), and treatments with H2SO4, ethylenediaminetetraacetic acid (EDTA) and NaOH, were examined, respectively.
27997408	1	64	theme	crucial	147:153	arg1	roles					155:159	crucial roles	147:159	crucial roles	147:159	Extracellular polymeric substances (EPS) play crucial roles in bio-aggregate formation and survival of bacterial cells.
27997408	2	65	from	MR-1	311:314	arg1	extraction					273:282	EPS extraction	269:282	EPS extraction from Shewanella oneidensis MR-1	269:314	To develop an effective but harmless method for EPS extraction from Shewanella oneidensis MR-1, five extraction methods, i.e. centrifugation (control), heating (40, 45, 50, and 60 °C), and treatments with H2SO4, ethylenediaminetetraacetic acid (EDTA) and NaOH, were examined, respectively.
27997408	4	66	contain	had	815:817	arg2	yield					838:842	a relatively lower yield	819:842	a relatively lower yield of EPS (proteins and polysaccharides yields of 5.15 and 1.30 mg g-1 dry cell, respectively)	819:934	Although EDTA treatment had a relatively lower yield of EPS (proteins and polysaccharides yields of 5.15 and 1.30 mg g-1 dry cell, respectively), cell lysis was barely found after EPS extraction.
27997408	4	66	contain	had	815:817	arg1	treatment					805:813	EDTA treatment	800:813	EDTA treatment	800:813	Although EDTA treatment had a relatively lower yield of EPS (proteins and polysaccharides yields of 5.15 and 1.30 mg g-1 dry cell, respectively), cell lysis was barely found after EPS extraction.
27997408	0	67	theme	oneidensis	84:93	arg1	MR-1					95:98	Shewanella oneidensis MR-1	73:98	Shewanella oneidensis MR-1	73:98	Effective methods for extracting extracellular polymeric substances from Shewanella oneidensis MR-1.
27997408	3	68	dep	g-1	763:765	arg1	cell					771:774	dry cell	767:774	dry cell	767:774	Results from scanning electron microscope and flow cytometric analyses indicate that MR-1 cells were severely broken by H2SO4, NaOH and heating temperature ≥45 °C. Proteins and polysaccharides in EPS extracted by heating at 40 °C were 7.12 and 1.60 mg g-1 dry cell, respectively.
27997408	4	69	theme	EPS	971:973	arg1	extraction					975:984	EPS extraction	971:984	EPS extraction	971:984	Although EDTA treatment had a relatively lower yield of EPS (proteins and polysaccharides yields of 5.15 and 1.30 mg g-1 dry cell, respectively), cell lysis was barely found after EPS extraction.
27997408	0	70	theme	Shewanella	73:82	arg1	MR-1					95:98	Shewanella oneidensis MR-1	73:98	Shewanella oneidensis MR-1	73:98	Effective methods for extracting extracellular polymeric substances from Shewanella oneidensis MR-1.
27997408	3	71	theme	cytometric	562:571	arg1	analyses					573:580	flow cytometric analyses	557:580	flow cytometric analyses	557:580	Results from scanning electron microscope and flow cytometric analyses indicate that MR-1 cells were severely broken by H2SO4, NaOH and heating temperature ≥45 °C. Proteins and polysaccharides in EPS extracted by heating at 40 °C were 7.12 and 1.60 mg g-1 dry cell, respectively.
26051725	7	0	theme	suspended	1214:1222	arg1	cells					1224:1228	suspended cells	1214:1228	suspended cells (S-180)	1214:1236	The results also revealed that there was selectively higher antitumor activity of the polysaccharides against suspended cells (S-180) than adherent ones (HT-29 and HCT-116).
26051725	7	0	theme	suspended	1214:1222	arg1	S-180					1231:1235	S-180	1231:1235	S-180	1231:1235	The results also revealed that there was selectively higher antitumor activity of the polysaccharides against suspended cells (S-180) than adherent ones (HT-29 and HCT-116).
26051725	4	1	theme	5mg/ml	852:857	arg1	dose					844:847	the dose	840:847	the dose of 5mg/ml	840:857	All the polysaccharide fractions exhibited antitumor activities against Sarcoma 180 (S-180) solid tumor cells and human colorectal cancer cell lines (HT-29 and HCT-116) in vitro at the dose of 5mg/ml.
26051725	3	2	theme	water-soluble	531:543	arg1	fractions					545:553	the water-soluble fractions	527:553	the water-soluble fractions	527:553	The results from high performance liquid chromatography (HPLC) revealed that the water-soluble fractions were heteroglucan containing mainly glucose (Glc), galactose (Gal) and mannose (Man) at various ratios.
26051725	3	2	theme	water-soluble	531:543	arg1	heteroglucan					560:571	heteroglucan	560:571	heteroglucan containing mainly glucose (Glc), galactose (Gal) and mannose (Man)	560:638	The results from high performance liquid chromatography (HPLC) revealed that the water-soluble fractions were heteroglucan containing mainly glucose (Glc), galactose (Gal) and mannose (Man) at various ratios.
26051725	6	3	theme	Gal	990:992	arg1	effects					979:985	The effects	975:985	The effects of Gal, Man and bound protein on the improvement of antitumor activities of polysaccharides	975:1077	The effects of Gal, Man and bound protein on the improvement of antitumor activities of polysaccharides might not be negligible.
26051725	6	3	theme	Gal	990:992	arg1	negligible					1092:1101	negligible	1092:1101	negligible	1092:1101	The effects of Gal, Man and bound protein on the improvement of antitumor activities of polysaccharides might not be negligible.
26051725	7	4	theme	polysaccharides	1190:1204	arg1	activity					1174:1181	higher antitumor activity	1157:1181	higher antitumor activity of the polysaccharides against suspended cells (S-180)	1157:1236	The results also revealed that there was selectively higher antitumor activity of the polysaccharides against suspended cells (S-180) than adherent ones (HT-29 and HCT-116).
26051725	4	5	theme	human	773:777	arg1	HCT-116					819:825	HCT-116	819:825	HCT-116	819:825	All the polysaccharide fractions exhibited antitumor activities against Sarcoma 180 (S-180) solid tumor cells and human colorectal cancer cell lines (HT-29 and HCT-116) in vitro at the dose of 5mg/ml.
26051725	4	5	theme	human	773:777	arg1	HT-29					809:813	HT-29	809:813	HT-29	809:813	All the polysaccharide fractions exhibited antitumor activities against Sarcoma 180 (S-180) solid tumor cells and human colorectal cancer cell lines (HT-29 and HCT-116) in vitro at the dose of 5mg/ml.
26051725	4	5	theme	human	773:777	arg1	lines					802:806	human colorectal cancer cell lines	773:806	human colorectal cancer cell lines (HT-29 and HCT-116)	773:826	All the polysaccharide fractions exhibited antitumor activities against Sarcoma 180 (S-180) solid tumor cells and human colorectal cancer cell lines (HT-29 and HCT-116) in vitro at the dose of 5mg/ml.
26051725	7	6	dep	ones	1252:1255	arg1	HT-29					1258:1262	HT-29	1258:1262	HT-29	1258:1262	The results also revealed that there was selectively higher antitumor activity of the polysaccharides against suspended cells (S-180) than adherent ones (HT-29 and HCT-116).
26051725	7	6	dep	ones	1252:1255	arg1	ones					1252:1255	adherent ones	1243:1255	adherent ones (HT-29 and HCT-116)	1243:1275	The results also revealed that there was selectively higher antitumor activity of the polysaccharides against suspended cells (S-180) than adherent ones (HT-29 and HCT-116).
26051725	7	6	dep	ones	1252:1255	arg1	HCT-116					1268:1274	HCT-116	1268:1274	HCT-116	1268:1274	The results also revealed that there was selectively higher antitumor activity of the polysaccharides against suspended cells (S-180) than adherent ones (HT-29 and HCT-116).
26051725	0	7	theme	fruiting	108:115	arg1	bodies					117:122	fruiting bodies	108:122	fruiting bodies	108:122	Relationship of chemical composition and cytotoxicity of water-soluble polysaccharides from Lentinus edodes fruiting bodies.
26051725	2	8	theme	molecular	389:397	arg1	weight					399:404	molecular weight	389:404	molecular weight	389:404	The fractions were analyzed for monosaccharide composition, molecular weight, and then tested for cytotoxicity activity.
26051725	1	9	theme	gel-permeation	292:305	arg1	GPC					323:325	GPC	323:325	GPC	323:325	Six water-soluble polysaccharides (S-WPLE-I-a, S-WPLE-I-b, S-WPLE-II-a, S-WPLE-II-b, S-WPLE-III-a and S-WPLE-III-b) were obtained from Lentinus edodes and purified by gel-permeation chromatography (GPC).
26051725	1	9	theme	gel-permeation	292:305	arg1	chromatography					307:320	gel-permeation chromatography	292:320	gel-permeation chromatography (GPC)	292:326	Six water-soluble polysaccharides (S-WPLE-I-a, S-WPLE-I-b, S-WPLE-II-a, S-WPLE-II-b, S-WPLE-III-a and S-WPLE-III-b) were obtained from Lentinus edodes and purified by gel-permeation chromatography (GPC).
26051725	3	10	from	ratios	651:656	arg1	fractions					545:553	the water-soluble fractions	527:553	the water-soluble fractions	527:553	The results from high performance liquid chromatography (HPLC) revealed that the water-soluble fractions were heteroglucan containing mainly glucose (Glc), galactose (Gal) and mannose (Man) at various ratios.
26051725	3	10	from	ratios	651:656	arg1	heteroglucan					560:571	heteroglucan	560:571	heteroglucan containing mainly glucose (Glc), galactose (Gal) and mannose (Man)	560:638	The results from high performance liquid chromatography (HPLC) revealed that the water-soluble fractions were heteroglucan containing mainly glucose (Glc), galactose (Gal) and mannose (Man) at various ratios.
26051725	5	11	theme	polysaccharides	892:906	arg1	related					913:919	related	913:919	related	913:919	The antitumor activities of the polysaccharides were related to their monosaccharide content and molecular weight.
26051725	5	11	theme	polysaccharides	892:906	arg1	activities					874:883	The antitumor activities	860:883	The antitumor activities of the polysaccharides	860:906	The antitumor activities of the polysaccharides were related to their monosaccharide content and molecular weight.
26051725	4	12	theme	cancer	790:795	arg1	HCT-116					819:825	HCT-116	819:825	HCT-116	819:825	All the polysaccharide fractions exhibited antitumor activities against Sarcoma 180 (S-180) solid tumor cells and human colorectal cancer cell lines (HT-29 and HCT-116) in vitro at the dose of 5mg/ml.
26051725	4	12	theme	cancer	790:795	arg1	HT-29					809:813	HT-29	809:813	HT-29	809:813	All the polysaccharide fractions exhibited antitumor activities against Sarcoma 180 (S-180) solid tumor cells and human colorectal cancer cell lines (HT-29 and HCT-116) in vitro at the dose of 5mg/ml.
26051725	4	12	theme	cancer	790:795	arg1	lines					802:806	human colorectal cancer cell lines	773:806	human colorectal cancer cell lines (HT-29 and HCT-116)	773:826	All the polysaccharide fractions exhibited antitumor activities against Sarcoma 180 (S-180) solid tumor cells and human colorectal cancer cell lines (HT-29 and HCT-116) in vitro at the dose of 5mg/ml.
26051725	6	13	theme	activities	1049:1058	arg1	improvement					1024:1034	the improvement	1020:1034	the improvement of antitumor activities of polysaccharides	1020:1077	The effects of Gal, Man and bound protein on the improvement of antitumor activities of polysaccharides might not be negligible.
26051725	3	14	theme	high	467:470	arg1	chromatography					491:504	high performance liquid chromatography	467:504	high performance liquid chromatography (HPLC)	467:511	The results from high performance liquid chromatography (HPLC) revealed that the water-soluble fractions were heteroglucan containing mainly glucose (Glc), galactose (Gal) and mannose (Man) at various ratios.
26051725	3	14	theme	high	467:470	arg1	HPLC					507:510	HPLC	507:510	HPLC	507:510	The results from high performance liquid chromatography (HPLC) revealed that the water-soluble fractions were heteroglucan containing mainly glucose (Glc), galactose (Gal) and mannose (Man) at various ratios.
26051725	0	15	from	cytotoxicity	41:52	arg1	Lentinus					92:99	Lentinus	92:99	Lentinus	92:99	Relationship of chemical composition and cytotoxicity of water-soluble polysaccharides from Lentinus edodes fruiting bodies.
26051725	6	16	from	effects	979:985	arg1	improvement					1024:1034	the improvement	1020:1034	the improvement of antitumor activities of polysaccharides	1020:1077	The effects of Gal, Man and bound protein on the improvement of antitumor activities of polysaccharides might not be negligible.
26051725	1	17	dep	polysaccharides	143:157	arg1	S-WPLE-III-a					210:221	S-WPLE-III-a	210:221	S-WPLE-III-a	210:221	Six water-soluble polysaccharides (S-WPLE-I-a, S-WPLE-I-b, S-WPLE-II-a, S-WPLE-II-b, S-WPLE-III-a and S-WPLE-III-b) were obtained from Lentinus edodes and purified by gel-permeation chromatography (GPC).
26051725	1	17	dep	polysaccharides	143:157	arg1	S-WPLE-I-b					172:181	S-WPLE-I-b	172:181	S-WPLE-I-b	172:181	Six water-soluble polysaccharides (S-WPLE-I-a, S-WPLE-I-b, S-WPLE-II-a, S-WPLE-II-b, S-WPLE-III-a and S-WPLE-III-b) were obtained from Lentinus edodes and purified by gel-permeation chromatography (GPC).
26051725	1	17	dep	polysaccharides	143:157	arg1	S-WPLE-II-a					184:194	S-WPLE-II-a	184:194	S-WPLE-II-a	184:194	Six water-soluble polysaccharides (S-WPLE-I-a, S-WPLE-I-b, S-WPLE-II-a, S-WPLE-II-b, S-WPLE-III-a and S-WPLE-III-b) were obtained from Lentinus edodes and purified by gel-permeation chromatography (GPC).
26051725	1	17	dep	polysaccharides	143:157	arg1	S-WPLE-III-b					227:238	S-WPLE-III-b	227:238	S-WPLE-III-b	227:238	Six water-soluble polysaccharides (S-WPLE-I-a, S-WPLE-I-b, S-WPLE-II-a, S-WPLE-II-b, S-WPLE-III-a and S-WPLE-III-b) were obtained from Lentinus edodes and purified by gel-permeation chromatography (GPC).
26051725	1	17	dep	polysaccharides	143:157	arg1	polysaccharides					143:157	Six water-soluble polysaccharides	125:157	Six water-soluble polysaccharides (S-WPLE-I-a, S-WPLE-I-b, S-WPLE-II-a, S-WPLE-II-b, S-WPLE-III-a and S-WPLE-III-b)	125:239	Six water-soluble polysaccharides (S-WPLE-I-a, S-WPLE-I-b, S-WPLE-II-a, S-WPLE-II-b, S-WPLE-III-a and S-WPLE-III-b) were obtained from Lentinus edodes and purified by gel-permeation chromatography (GPC).
26051725	1	17	dep	polysaccharides	143:157	arg1	S-WPLE-I-a					160:169	S-WPLE-I-a	160:169	S-WPLE-I-a	160:169	Six water-soluble polysaccharides (S-WPLE-I-a, S-WPLE-I-b, S-WPLE-II-a, S-WPLE-II-b, S-WPLE-III-a and S-WPLE-III-b) were obtained from Lentinus edodes and purified by gel-permeation chromatography (GPC).
26051725	1	17	dep	polysaccharides	143:157	arg1	S-WPLE-II-b					197:207	S-WPLE-II-b	197:207	S-WPLE-II-b	197:207	Six water-soluble polysaccharides (S-WPLE-I-a, S-WPLE-I-b, S-WPLE-II-a, S-WPLE-II-b, S-WPLE-III-a and S-WPLE-III-b) were obtained from Lentinus edodes and purified by gel-permeation chromatography (GPC).
26051725	3	18	theme	performance	472:482	arg1	chromatography					491:504	high performance liquid chromatography	467:504	high performance liquid chromatography (HPLC)	467:511	The results from high performance liquid chromatography (HPLC) revealed that the water-soluble fractions were heteroglucan containing mainly glucose (Glc), galactose (Gal) and mannose (Man) at various ratios.
26051725	3	18	theme	performance	472:482	arg1	HPLC					507:510	HPLC	507:510	HPLC	507:510	The results from high performance liquid chromatography (HPLC) revealed that the water-soluble fractions were heteroglucan containing mainly glucose (Glc), galactose (Gal) and mannose (Man) at various ratios.
26051725	7	19	theme	antitumor	1164:1172	arg1	activity					1174:1181	higher antitumor activity	1157:1181	higher antitumor activity of the polysaccharides against suspended cells (S-180)	1157:1236	The results also revealed that there was selectively higher antitumor activity of the polysaccharides against suspended cells (S-180) than adherent ones (HT-29 and HCT-116).
26051725	4	20	theme	solid	751:755	arg1	cells					763:767	Sarcoma 180 (S-180) solid tumor cells	731:767	Sarcoma 180 (S-180) solid tumor cells	731:767	All the polysaccharide fractions exhibited antitumor activities against Sarcoma 180 (S-180) solid tumor cells and human colorectal cancer cell lines (HT-29 and HCT-116) in vitro at the dose of 5mg/ml.
26051725	4	21	dep	lines	802:806	arg1	HCT-116					819:825	HCT-116	819:825	HCT-116	819:825	All the polysaccharide fractions exhibited antitumor activities against Sarcoma 180 (S-180) solid tumor cells and human colorectal cancer cell lines (HT-29 and HCT-116) in vitro at the dose of 5mg/ml.
26051725	4	21	dep	lines	802:806	arg1	HT-29					809:813	HT-29	809:813	HT-29	809:813	All the polysaccharide fractions exhibited antitumor activities against Sarcoma 180 (S-180) solid tumor cells and human colorectal cancer cell lines (HT-29 and HCT-116) in vitro at the dose of 5mg/ml.
26051725	4	21	dep	lines	802:806	arg1	lines					802:806	human colorectal cancer cell lines	773:806	human colorectal cancer cell lines (HT-29 and HCT-116)	773:826	All the polysaccharide fractions exhibited antitumor activities against Sarcoma 180 (S-180) solid tumor cells and human colorectal cancer cell lines (HT-29 and HCT-116) in vitro at the dose of 5mg/ml.
26051725	5	22	theme	monosaccharide	930:943	arg1	content					945:951	their monosaccharide content	924:951	their monosaccharide content	924:951	The antitumor activities of the polysaccharides were related to their monosaccharide content and molecular weight.
26051725	4	23	theme	polysaccharide	667:680	arg1	fractions					682:690	All the polysaccharide fractions	659:690	All the polysaccharide fractions	659:690	All the polysaccharide fractions exhibited antitumor activities against Sarcoma 180 (S-180) solid tumor cells and human colorectal cancer cell lines (HT-29 and HCT-116) in vitro at the dose of 5mg/ml.
26051725	0	24	theme	composition	25:35	arg1	Relationship					0:11	Relationship	0:11	Relationship of chemical composition and cytotoxicity of water-soluble polysaccharides from Lentinus	0:99	Relationship of chemical composition and cytotoxicity of water-soluble polysaccharides from Lentinus edodes fruiting bodies.
26051725	7	25	theme	adherent	1243:1250	arg1	HT-29					1258:1262	HT-29	1258:1262	HT-29	1258:1262	The results also revealed that there was selectively higher antitumor activity of the polysaccharides against suspended cells (S-180) than adherent ones (HT-29 and HCT-116).
26051725	7	25	theme	adherent	1243:1250	arg1	ones					1252:1255	adherent ones	1243:1255	adherent ones (HT-29 and HCT-116)	1243:1275	The results also revealed that there was selectively higher antitumor activity of the polysaccharides against suspended cells (S-180) than adherent ones (HT-29 and HCT-116).
26051725	7	25	theme	adherent	1243:1250	arg1	HCT-116					1268:1274	HCT-116	1268:1274	HCT-116	1268:1274	The results also revealed that there was selectively higher antitumor activity of the polysaccharides against suspended cells (S-180) than adherent ones (HT-29 and HCT-116).
26051725	5	26	theme	molecular	957:965	arg1	weight					967:972	molecular weight	957:972	molecular weight	957:972	The antitumor activities of the polysaccharides were related to their monosaccharide content and molecular weight.
26051725	0	27	theme	chemical	16:23	arg1	composition					25:35	chemical composition	16:35	chemical composition	16:35	Relationship of chemical composition and cytotoxicity of water-soluble polysaccharides from Lentinus edodes fruiting bodies.
26051725	4	28	theme	tumor	757:761	arg1	cells					763:767	Sarcoma 180 (S-180) solid tumor cells	731:767	Sarcoma 180 (S-180) solid tumor cells	731:767	All the polysaccharide fractions exhibited antitumor activities against Sarcoma 180 (S-180) solid tumor cells and human colorectal cancer cell lines (HT-29 and HCT-116) in vitro at the dose of 5mg/ml.
26051725	5	29	theme	antitumor	864:872	arg1	related					913:919	related	913:919	related	913:919	The antitumor activities of the polysaccharides were related to their monosaccharide content and molecular weight.
26051725	5	29	theme	antitumor	864:872	arg1	activities					874:883	The antitumor activities	860:883	The antitumor activities of the polysaccharides	860:906	The antitumor activities of the polysaccharides were related to their monosaccharide content and molecular weight.
26051725	0	30	theme	cytotoxicity	41:52	arg1	Relationship					0:11	Relationship	0:11	Relationship of chemical composition and cytotoxicity of water-soluble polysaccharides from Lentinus	0:99	Relationship of chemical composition and cytotoxicity of water-soluble polysaccharides from Lentinus edodes fruiting bodies.
26051725	0	31	from	Lentinus	92:99	arg1	cytotoxicity					41:52	cytotoxicity	41:52	cytotoxicity	41:52	Relationship of chemical composition and cytotoxicity of water-soluble polysaccharides from Lentinus edodes fruiting bodies.
26051725	0	31	from	Lentinus	92:99	arg1	polysaccharides					71:85	water-soluble polysaccharides	57:85	water-soluble polysaccharides from Lentinus	57:99	Relationship of chemical composition and cytotoxicity of water-soluble polysaccharides from Lentinus edodes fruiting bodies.
26051725	0	31	from	Lentinus	92:99	arg1	composition					25:35	chemical composition	16:35	chemical composition	16:35	Relationship of chemical composition and cytotoxicity of water-soluble polysaccharides from Lentinus edodes fruiting bodies.
26051725	6	32	theme	antitumor	1039:1047	arg1	activities					1049:1058	antitumor activities	1039:1058	antitumor activities of polysaccharides	1039:1077	The effects of Gal, Man and bound protein on the improvement of antitumor activities of polysaccharides might not be negligible.
26051725	3	33	theme	liquid	484:489	arg1	chromatography					491:504	high performance liquid chromatography	467:504	high performance liquid chromatography (HPLC)	467:511	The results from high performance liquid chromatography (HPLC) revealed that the water-soluble fractions were heteroglucan containing mainly glucose (Glc), galactose (Gal) and mannose (Man) at various ratios.
26051725	3	33	theme	liquid	484:489	arg1	HPLC					507:510	HPLC	507:510	HPLC	507:510	The results from high performance liquid chromatography (HPLC) revealed that the water-soluble fractions were heteroglucan containing mainly glucose (Glc), galactose (Gal) and mannose (Man) at various ratios.
26051725	6	34	theme	polysaccharides	1063:1077	arg1	activities					1049:1058	antitumor activities	1039:1058	antitumor activities of polysaccharides	1039:1077	The effects of Gal, Man and bound protein on the improvement of antitumor activities of polysaccharides might not be negligible.
26051725	2	35	theme	cytotoxicity	427:438	arg1	activity					440:447	cytotoxicity activity	427:447	cytotoxicity activity	427:447	The fractions were analyzed for monosaccharide composition, molecular weight, and then tested for cytotoxicity activity.
26051725	0	36	theme	water-soluble	57:69	arg1	polysaccharides					71:85	water-soluble polysaccharides	57:85	water-soluble polysaccharides from Lentinus	57:99	Relationship of chemical composition and cytotoxicity of water-soluble polysaccharides from Lentinus edodes fruiting bodies.
26051725	3	37	contain	containing	573:582	arg1	fractions					545:553	the water-soluble fractions	527:553	the water-soluble fractions	527:553	The results from high performance liquid chromatography (HPLC) revealed that the water-soluble fractions were heteroglucan containing mainly glucose (Glc), galactose (Gal) and mannose (Man) at various ratios.
26051725	3	37	contain	containing	573:582	arg2	Man					635:637	Man	635:637	Man	635:637	The results from high performance liquid chromatography (HPLC) revealed that the water-soluble fractions were heteroglucan containing mainly glucose (Glc), galactose (Gal) and mannose (Man) at various ratios.
26051725	3	37	contain	containing	573:582	arg1	heteroglucan					560:571	heteroglucan	560:571	heteroglucan containing mainly glucose (Glc), galactose (Gal) and mannose (Man)	560:638	The results from high performance liquid chromatography (HPLC) revealed that the water-soluble fractions were heteroglucan containing mainly glucose (Glc), galactose (Gal) and mannose (Man) at various ratios.
26051725	3	37	contain	containing	573:582	arg2	Gal					617:619	Gal	617:619	Gal	617:619	The results from high performance liquid chromatography (HPLC) revealed that the water-soluble fractions were heteroglucan containing mainly glucose (Glc), galactose (Gal) and mannose (Man) at various ratios.
26051725	3	37	contain	containing	573:582	arg2	glucose					591:597	glucose	591:597	glucose (Glc)	591:603	The results from high performance liquid chromatography (HPLC) revealed that the water-soluble fractions were heteroglucan containing mainly glucose (Glc), galactose (Gal) and mannose (Man) at various ratios.
26051725	3	37	contain	containing	573:582	arg2	Glc					600:602	Glc	600:602	Glc	600:602	The results from high performance liquid chromatography (HPLC) revealed that the water-soluble fractions were heteroglucan containing mainly glucose (Glc), galactose (Gal) and mannose (Man) at various ratios.
26051725	3	37	contain	containing	573:582	arg2	galactose					606:614	galactose	606:614	galactose (Gal)	606:620	The results from high performance liquid chromatography (HPLC) revealed that the water-soluble fractions were heteroglucan containing mainly glucose (Glc), galactose (Gal) and mannose (Man) at various ratios.
26051725	3	37	contain	containing	573:582	arg2	mannose					626:632	mannose	626:632	mannose (Man)	626:638	The results from high performance liquid chromatography (HPLC) revealed that the water-soluble fractions were heteroglucan containing mainly glucose (Glc), galactose (Gal) and mannose (Man) at various ratios.
26051725	3	38	theme	various	643:649	arg1	ratios					651:656	various ratios	643:656	various ratios	643:656	The results from high performance liquid chromatography (HPLC) revealed that the water-soluble fractions were heteroglucan containing mainly glucose (Glc), galactose (Gal) and mannose (Man) at various ratios.
26051725	3	39	from	chromatography	491:504	arg1	results					454:460	The results	450:460	The results from high performance liquid chromatography (HPLC)	450:511	The results from high performance liquid chromatography (HPLC) revealed that the water-soluble fractions were heteroglucan containing mainly glucose (Glc), galactose (Gal) and mannose (Man) at various ratios.
26051725	1	40	theme	water-soluble	129:141	arg1	S-WPLE-III-a					210:221	S-WPLE-III-a	210:221	S-WPLE-III-a	210:221	Six water-soluble polysaccharides (S-WPLE-I-a, S-WPLE-I-b, S-WPLE-II-a, S-WPLE-II-b, S-WPLE-III-a and S-WPLE-III-b) were obtained from Lentinus edodes and purified by gel-permeation chromatography (GPC).
26051725	1	40	theme	water-soluble	129:141	arg1	S-WPLE-I-b					172:181	S-WPLE-I-b	172:181	S-WPLE-I-b	172:181	Six water-soluble polysaccharides (S-WPLE-I-a, S-WPLE-I-b, S-WPLE-II-a, S-WPLE-II-b, S-WPLE-III-a and S-WPLE-III-b) were obtained from Lentinus edodes and purified by gel-permeation chromatography (GPC).
26051725	1	40	theme	water-soluble	129:141	arg1	S-WPLE-II-a					184:194	S-WPLE-II-a	184:194	S-WPLE-II-a	184:194	Six water-soluble polysaccharides (S-WPLE-I-a, S-WPLE-I-b, S-WPLE-II-a, S-WPLE-II-b, S-WPLE-III-a and S-WPLE-III-b) were obtained from Lentinus edodes and purified by gel-permeation chromatography (GPC).
26051725	1	40	theme	water-soluble	129:141	arg1	S-WPLE-III-b					227:238	S-WPLE-III-b	227:238	S-WPLE-III-b	227:238	Six water-soluble polysaccharides (S-WPLE-I-a, S-WPLE-I-b, S-WPLE-II-a, S-WPLE-II-b, S-WPLE-III-a and S-WPLE-III-b) were obtained from Lentinus edodes and purified by gel-permeation chromatography (GPC).
26051725	1	40	theme	water-soluble	129:141	arg1	polysaccharides					143:157	Six water-soluble polysaccharides	125:157	Six water-soluble polysaccharides (S-WPLE-I-a, S-WPLE-I-b, S-WPLE-II-a, S-WPLE-II-b, S-WPLE-III-a and S-WPLE-III-b)	125:239	Six water-soluble polysaccharides (S-WPLE-I-a, S-WPLE-I-b, S-WPLE-II-a, S-WPLE-II-b, S-WPLE-III-a and S-WPLE-III-b) were obtained from Lentinus edodes and purified by gel-permeation chromatography (GPC).
26051725	1	40	theme	water-soluble	129:141	arg1	S-WPLE-I-a					160:169	S-WPLE-I-a	160:169	S-WPLE-I-a	160:169	Six water-soluble polysaccharides (S-WPLE-I-a, S-WPLE-I-b, S-WPLE-II-a, S-WPLE-II-b, S-WPLE-III-a and S-WPLE-III-b) were obtained from Lentinus edodes and purified by gel-permeation chromatography (GPC).
26051725	1	40	theme	water-soluble	129:141	arg1	S-WPLE-II-b					197:207	S-WPLE-II-b	197:207	S-WPLE-II-b	197:207	Six water-soluble polysaccharides (S-WPLE-I-a, S-WPLE-I-b, S-WPLE-II-a, S-WPLE-II-b, S-WPLE-III-a and S-WPLE-III-b) were obtained from Lentinus edodes and purified by gel-permeation chromatography (GPC).
26051725	2	41	theme	monosaccharide	361:374	arg1	composition					376:386	monosaccharide composition	361:386	monosaccharide composition	361:386	The fractions were analyzed for monosaccharide composition, molecular weight, and then tested for cytotoxicity activity.
26051725	2	42	dep	then	411:414	arg1	tested					416:421	tested	416:421	tested for cytotoxicity activity	416:447	The fractions were analyzed for monosaccharide composition, molecular weight, and then tested for cytotoxicity activity.
26051725	4	43	dep	solid	751:755	arg1	Sarcoma					731:737	Sarcoma 180	731:741	Sarcoma 180	731:741	All the polysaccharide fractions exhibited antitumor activities against Sarcoma 180 (S-180) solid tumor cells and human colorectal cancer cell lines (HT-29 and HCT-116) in vitro at the dose of 5mg/ml.
26051725	6	44	theme	protein	1009:1015	arg1	effects					979:985	The effects	975:985	The effects of Gal, Man and bound protein on the improvement of antitumor activities of polysaccharides	975:1077	The effects of Gal, Man and bound protein on the improvement of antitumor activities of polysaccharides might not be negligible.
26051725	6	44	theme	protein	1009:1015	arg1	negligible					1092:1101	negligible	1092:1101	negligible	1092:1101	The effects of Gal, Man and bound protein on the improvement of antitumor activities of polysaccharides might not be negligible.
26051725	4	45	theme	colorectal	779:788	arg1	HCT-116					819:825	HCT-116	819:825	HCT-116	819:825	All the polysaccharide fractions exhibited antitumor activities against Sarcoma 180 (S-180) solid tumor cells and human colorectal cancer cell lines (HT-29 and HCT-116) in vitro at the dose of 5mg/ml.
26051725	4	45	theme	colorectal	779:788	arg1	HT-29					809:813	HT-29	809:813	HT-29	809:813	All the polysaccharide fractions exhibited antitumor activities against Sarcoma 180 (S-180) solid tumor cells and human colorectal cancer cell lines (HT-29 and HCT-116) in vitro at the dose of 5mg/ml.
26051725	4	45	theme	colorectal	779:788	arg1	lines					802:806	human colorectal cancer cell lines	773:806	human colorectal cancer cell lines (HT-29 and HCT-116)	773:826	All the polysaccharide fractions exhibited antitumor activities against Sarcoma 180 (S-180) solid tumor cells and human colorectal cancer cell lines (HT-29 and HCT-116) in vitro at the dose of 5mg/ml.
26051725	4	46	theme	cell	797:800	arg1	HCT-116					819:825	HCT-116	819:825	HCT-116	819:825	All the polysaccharide fractions exhibited antitumor activities against Sarcoma 180 (S-180) solid tumor cells and human colorectal cancer cell lines (HT-29 and HCT-116) in vitro at the dose of 5mg/ml.
26051725	4	46	theme	cell	797:800	arg1	HT-29					809:813	HT-29	809:813	HT-29	809:813	All the polysaccharide fractions exhibited antitumor activities against Sarcoma 180 (S-180) solid tumor cells and human colorectal cancer cell lines (HT-29 and HCT-116) in vitro at the dose of 5mg/ml.
26051725	4	46	theme	cell	797:800	arg1	lines					802:806	human colorectal cancer cell lines	773:806	human colorectal cancer cell lines (HT-29 and HCT-116)	773:826	All the polysaccharide fractions exhibited antitumor activities against Sarcoma 180 (S-180) solid tumor cells and human colorectal cancer cell lines (HT-29 and HCT-116) in vitro at the dose of 5mg/ml.
26051725	6	47	theme	bound	1003:1007	arg1	protein					1009:1015	bound protein	1003:1015	bound protein	1003:1015	The effects of Gal, Man and bound protein on the improvement of antitumor activities of polysaccharides might not be negligible.
26051725	4	48	theme	antitumor	702:710	arg1	activities					712:721	antitumor activities	702:721	antitumor activities against Sarcoma 180 (S-180) solid tumor cells and human colorectal cancer cell lines (HT-29 and HCT-116)	702:826	All the polysaccharide fractions exhibited antitumor activities against Sarcoma 180 (S-180) solid tumor cells and human colorectal cancer cell lines (HT-29 and HCT-116) in vitro at the dose of 5mg/ml.
26051725	1	49	theme	Lentinus	260:267	arg1	edodes					269:274	Lentinus edodes	260:274	Lentinus edodes	260:274	Six water-soluble polysaccharides (S-WPLE-I-a, S-WPLE-I-b, S-WPLE-II-a, S-WPLE-II-b, S-WPLE-III-a and S-WPLE-III-b) were obtained from Lentinus edodes and purified by gel-permeation chromatography (GPC).
26051725	0	50	theme	polysaccharides	71:85	arg1	cytotoxicity					41:52	cytotoxicity	41:52	cytotoxicity	41:52	Relationship of chemical composition and cytotoxicity of water-soluble polysaccharides from Lentinus edodes fruiting bodies.
26051725	0	50	theme	polysaccharides	71:85	arg1	composition					25:35	chemical composition	16:35	chemical composition	16:35	Relationship of chemical composition and cytotoxicity of water-soluble polysaccharides from Lentinus edodes fruiting bodies.
26051725	0	51	from	composition	25:35	arg1	Lentinus					92:99	Lentinus	92:99	Lentinus	92:99	Relationship of chemical composition and cytotoxicity of water-soluble polysaccharides from Lentinus edodes fruiting bodies.
26051725	7	52	theme	higher	1157:1162	arg1	activity					1174:1181	higher antitumor activity	1157:1181	higher antitumor activity of the polysaccharides against suspended cells (S-180)	1157:1236	The results also revealed that there was selectively higher antitumor activity of the polysaccharides against suspended cells (S-180) than adherent ones (HT-29 and HCT-116).
26051725	6	53	theme	Man	995:997	arg1	effects					979:985	The effects	975:985	The effects of Gal, Man and bound protein on the improvement of antitumor activities of polysaccharides	975:1077	The effects of Gal, Man and bound protein on the improvement of antitumor activities of polysaccharides might not be negligible.
26051725	6	53	theme	Man	995:997	arg1	negligible					1092:1101	negligible	1092:1101	negligible	1092:1101	The effects of Gal, Man and bound protein on the improvement of antitumor activities of polysaccharides might not be negligible.
28323423	7	0	theme	several	1355:1361	arg1	times					1363:1367	several times	1355:1367	several times with the same capsule	1355:1389	The high integrity of cell wall was due to the CNF and the ON/OFF alteration of the permeability properties, and subsequent loading/unloading of molecules, could be repeated several times with the same capsule demonstrating a robust microcontainer with controllable permeability properties.
28323423	7	1	theme	same	1378:1381	arg1	capsule					1383:1389	the same capsule	1374:1389	the same capsule	1374:1389	The high integrity of cell wall was due to the CNF and the ON/OFF alteration of the permeability properties, and subsequent loading/unloading of molecules, could be repeated several times with the same capsule demonstrating a robust microcontainer with controllable permeability properties.
28323423	1	2	theme	fast	190:193	arg1	behavior					243:250	fast, stimuli-triggered, and switchable permeability behavior	190:250	fast, stimuli-triggered, and switchable permeability behavior	190:250	Green, all-polysaccharide based microcapsules with mechanically robust capsule walls and fast, stimuli-triggered, and switchable permeability behavior show great promise in applications based on selective and timed permeability.
28323423	2	3	theme	mechanical	503:512	arg1	properties					514:523	high mechanical properties	498:523	high mechanical properties	498:523	Taking a cue from nature, the build-up and composition of plant primary cell walls inspired the capsule wall assembly, because the primary cell walls in plants exhibit high mechanical properties despite being in a highly hydrated state, primarily owing to cellulose microfibrils.
28323423	2	4	from	walls	474:478	arg1	plants					483:488	plants	483:488	plants	483:488	Taking a cue from nature, the build-up and composition of plant primary cell walls inspired the capsule wall assembly, because the primary cell walls in plants exhibit high mechanical properties despite being in a highly hydrated state, primarily owing to cellulose microfibrils.
28323423	4	5	theme	labeled	860:866	arg1	dextrans					868:875	labeled dextrans	860:875	labeled dextrans with a hydrodynamic diameter of ∼6.6 nm	860:915	In water, the capsule wall was permeable to labeled dextrans with a hydrodynamic diameter of ∼6.6 nm.
28323423	7	6	theme	high	1185:1188	arg1	integrity					1190:1198	The high integrity	1181:1198	The high integrity of cell wall	1181:1211	The high integrity of cell wall was due to the CNF and the ON/OFF alteration of the permeability properties, and subsequent loading/unloading of molecules, could be repeated several times with the same capsule demonstrating a robust microcontainer with controllable permeability properties.
28323423	7	6	theme	high	1185:1188	arg1	due					1217:1219	due	1217:1219	due	1217:1219	The high integrity of cell wall was due to the CNF and the ON/OFF alteration of the permeability properties, and subsequent loading/unloading of molecules, could be repeated several times with the same capsule demonstrating a robust microcontainer with controllable permeability properties.
28323423	7	7	with	microcontainer	1414:1427	arg1	properties					1460:1469	controllable permeability properties	1434:1469	controllable permeability properties	1434:1469	The high integrity of cell wall was due to the CNF and the ON/OFF alteration of the permeability properties, and subsequent loading/unloading of molecules, could be repeated several times with the same capsule demonstrating a robust microcontainer with controllable permeability properties.
28323423	1	8	theme	selective	296:304	arg1	permeability					316:327	selective and timed permeability	296:327	selective and timed permeability	296:327	Green, all-polysaccharide based microcapsules with mechanically robust capsule walls and fast, stimuli-triggered, and switchable permeability behavior show great promise in applications based on selective and timed permeability.
28323423	2	9	theme	hydrated	551:558	arg1	state					560:564	a highly hydrated state	542:564	a highly hydrated state	542:564	Taking a cue from nature, the build-up and composition of plant primary cell walls inspired the capsule wall assembly, because the primary cell walls in plants exhibit high mechanical properties despite being in a highly hydrated state, primarily owing to cellulose microfibrils.
28323423	0	10	dep	Microcapsules	27:39	arg1	Based					41:45	Based	41:45	Microcapsules Based on Cellulose Nanofibers with Switchable Permeability	27:98	Bioinspired Layer-by-Layer Microcapsules Based on Cellulose Nanofibers with Switchable Permeability.
28323423	2	11	from	nature	348:353	arg1	cue					339:341	a cue	337:341	a cue from nature	337:353	Taking a cue from nature, the build-up and composition of plant primary cell walls inspired the capsule wall assembly, because the primary cell walls in plants exhibit high mechanical properties despite being in a highly hydrated state, primarily owing to cellulose microfibrils.
28323423	6	12	theme	NaCl	1114:1117	arg1	removal					1103:1109	removal	1103:1109	removal of NaCl	1103:1117	However, the porosity could be restored to its original state by removal of NaCl, by which permeants became trapped inside the capsule's core.
28323423	3	13	theme	plant	741:745	arg1	xyloglucan					798:807	xyloglucan	798:807	xyloglucan	798:807	The microcapsules (16 ± 4 μm in diameter) were fabricated using the layer-by-layer technique on sacrificial CaCO3 templates, using plant polysaccharides (pectin, cellulose nanofibers, and xyloglucan) only.
28323423	3	13	theme	plant	741:745	arg1	pectin					764:769	pectin	764:769	pectin	764:769	The microcapsules (16 ± 4 μm in diameter) were fabricated using the layer-by-layer technique on sacrificial CaCO3 templates, using plant polysaccharides (pectin, cellulose nanofibers, and xyloglucan) only.
28323423	3	13	theme	plant	741:745	arg1	nanofibers					782:791	cellulose nanofibers	772:791	cellulose nanofibers	772:791	The microcapsules (16 ± 4 μm in diameter) were fabricated using the layer-by-layer technique on sacrificial CaCO3 templates, using plant polysaccharides (pectin, cellulose nanofibers, and xyloglucan) only.
28323423	3	13	theme	plant	741:745	arg1	polysaccharides					747:761	plant polysaccharides	741:761	plant polysaccharides (pectin, cellulose nanofibers, and xyloglucan)	741:808	The microcapsules (16 ± 4 μm in diameter) were fabricated using the layer-by-layer technique on sacrificial CaCO3 templates, using plant polysaccharides (pectin, cellulose nanofibers, and xyloglucan) only.
28323423	0	14	theme	Bioinspired	0:10	arg1	Layer-by-Layer					12:25	Bioinspired Layer-by-Layer	0:25	Bioinspired Layer-by-Layer	0:25	Bioinspired Layer-by-Layer Microcapsules Based on Cellulose Nanofibers with Switchable Permeability.
28323423	1	15	theme	timed	310:314	arg1	permeability					316:327	selective and timed permeability	296:327	selective and timed permeability	296:327	Green, all-polysaccharide based microcapsules with mechanically robust capsule walls and fast, stimuli-triggered, and switchable permeability behavior show great promise in applications based on selective and timed permeability.
28323423	4	16	from	permeable	847:855	arg1	water					819:823	water	819:823	water	819:823	In water, the capsule wall was permeable to labeled dextrans with a hydrodynamic diameter of ∼6.6 nm.
28323423	1	17	theme	stimuli-triggered	196:212	arg1	behavior					243:250	fast, stimuli-triggered, and switchable permeability behavior	190:250	fast, stimuli-triggered, and switchable permeability behavior	190:250	Green, all-polysaccharide based microcapsules with mechanically robust capsule walls and fast, stimuli-triggered, and switchable permeability behavior show great promise in applications based on selective and timed permeability.
28323423	2	18	theme	high	498:501	arg1	properties					514:523	high mechanical properties	498:523	high mechanical properties	498:523	Taking a cue from nature, the build-up and composition of plant primary cell walls inspired the capsule wall assembly, because the primary cell walls in plants exhibit high mechanical properties despite being in a highly hydrated state, primarily owing to cellulose microfibrils.
28323423	1	19	theme	Green	101:105	arg1	microcapsules					133:145	Green, all-polysaccharide based microcapsules	101:145	microcapsules	133:145	Green, all-polysaccharide based microcapsules with mechanically robust capsule walls and fast, stimuli-triggered, and switchable permeability behavior show great promise in applications based on selective and timed permeability.
28323423	3	20	from	μm	636:637	arg1	diameter					642:649	diameter	642:649	diameter	642:649	The microcapsules (16 ± 4 μm in diameter) were fabricated using the layer-by-layer technique on sacrificial CaCO3 templates, using plant polysaccharides (pectin, cellulose nanofibers, and xyloglucan) only.
28323423	3	21	dep	microcapsules	614:626	arg1	μm					636:637	16 ± 4 μm	629:637	16 ± 4 μm in diameter	629:649	The microcapsules (16 ± 4 μm in diameter) were fabricated using the layer-by-layer technique on sacrificial CaCO3 templates, using plant polysaccharides (pectin, cellulose nanofibers, and xyloglucan) only.
28323423	3	22	from	technique	693:701	arg1	templates					724:732	sacrificial CaCO3 templates	706:732	sacrificial CaCO3 templates	706:732	The microcapsules (16 ± 4 μm in diameter) were fabricated using the layer-by-layer technique on sacrificial CaCO3 templates, using plant polysaccharides (pectin, cellulose nanofibers, and xyloglucan) only.
28323423	2	23	dep	build-up	360:367	arg1	the					356:358	the	356:358	the	356:358	Taking a cue from nature, the build-up and composition of plant primary cell walls inspired the capsule wall assembly, because the primary cell walls in plants exhibit high mechanical properties despite being in a highly hydrated state, primarily owing to cellulose microfibrils.
28323423	7	24	theme	wall	1208:1211	arg1	integrity					1190:1198	The high integrity	1181:1198	The high integrity of cell wall	1181:1211	The high integrity of cell wall was due to the CNF and the ON/OFF alteration of the permeability properties, and subsequent loading/unloading of molecules, could be repeated several times with the same capsule demonstrating a robust microcontainer with controllable permeability properties.
28323423	7	24	theme	wall	1208:1211	arg1	due					1217:1219	due	1217:1219	due	1217:1219	The high integrity of cell wall was due to the CNF and the ON/OFF alteration of the permeability properties, and subsequent loading/unloading of molecules, could be repeated several times with the same capsule demonstrating a robust microcontainer with controllable permeability properties.
28323423	2	25	theme	cell	469:472	arg1	walls					474:478	the primary cell walls	457:478	the primary cell walls in plants	457:488	Taking a cue from nature, the build-up and composition of plant primary cell walls inspired the capsule wall assembly, because the primary cell walls in plants exhibit high mechanical properties despite being in a highly hydrated state, primarily owing to cellulose microfibrils.
28323423	7	26	theme	permeability	1447:1458	arg1	properties					1460:1469	controllable permeability properties	1434:1469	controllable permeability properties	1434:1469	The high integrity of cell wall was due to the CNF and the ON/OFF alteration of the permeability properties, and subsequent loading/unloading of molecules, could be repeated several times with the same capsule demonstrating a robust microcontainer with controllable permeability properties.
28323423	1	27	theme	all-polysaccharide	108:125	arg1	microcapsules					133:145	Green, all-polysaccharide based microcapsules	101:145	microcapsules	133:145	Green, all-polysaccharide based microcapsules with mechanically robust capsule walls and fast, stimuli-triggered, and switchable permeability behavior show great promise in applications based on selective and timed permeability.
28323423	1	28	theme	switchable	219:228	arg1	behavior					243:250	fast, stimuli-triggered, and switchable permeability behavior	190:250	fast, stimuli-triggered, and switchable permeability behavior	190:250	Green, all-polysaccharide based microcapsules with mechanically robust capsule walls and fast, stimuli-triggered, and switchable permeability behavior show great promise in applications based on selective and timed permeability.
28323423	2	29	theme	primary	461:467	arg1	walls					474:478	the primary cell walls	457:478	the primary cell walls in plants	457:488	Taking a cue from nature, the build-up and composition of plant primary cell walls inspired the capsule wall assembly, because the primary cell walls in plants exhibit high mechanical properties despite being in a highly hydrated state, primarily owing to cellulose microfibrils.
28323423	3	30	theme	cellulose	772:780	arg1	nanofibers					782:791	cellulose nanofibers	772:791	cellulose nanofibers	772:791	The microcapsules (16 ± 4 μm in diameter) were fabricated using the layer-by-layer technique on sacrificial CaCO3 templates, using plant polysaccharides (pectin, cellulose nanofibers, and xyloglucan) only.
28323423	3	30	theme	cellulose	772:780	arg1	polysaccharides					747:761	plant polysaccharides	741:761	plant polysaccharides (pectin, cellulose nanofibers, and xyloglucan)	741:808	The microcapsules (16 ± 4 μm in diameter) were fabricated using the layer-by-layer technique on sacrificial CaCO3 templates, using plant polysaccharides (pectin, cellulose nanofibers, and xyloglucan) only.
28323423	7	31	theme	ON/OFF	1240:1245	arg1	alteration					1247:1256	the ON/OFF alteration	1236:1256	the ON/OFF alteration of the permeability properties	1236:1287	The high integrity of cell wall was due to the CNF and the ON/OFF alteration of the permeability properties, and subsequent loading/unloading of molecules, could be repeated several times with the same capsule demonstrating a robust microcontainer with controllable permeability properties.
28323423	1	32	theme	based	127:131	arg1	microcapsules					133:145	Green, all-polysaccharide based microcapsules	101:145	microcapsules	133:145	Green, all-polysaccharide based microcapsules with mechanically robust capsule walls and fast, stimuli-triggered, and switchable permeability behavior show great promise in applications based on selective and timed permeability.
28323423	1	33	theme	permeability	230:241	arg1	behavior					243:250	fast, stimuli-triggered, and switchable permeability behavior	190:250	fast, stimuli-triggered, and switchable permeability behavior	190:250	Green, all-polysaccharide based microcapsules with mechanically robust capsule walls and fast, stimuli-triggered, and switchable permeability behavior show great promise in applications based on selective and timed permeability.
28323423	4	34	theme	nm	914:915	arg1	diameter					897:904	a hydrodynamic diameter	882:904	a hydrodynamic diameter of ∼6.6 nm	882:915	In water, the capsule wall was permeable to labeled dextrans with a hydrodynamic diameter of ∼6.6 nm.
28323423	2	35	theme	cell	402:405	arg1	walls					407:411	plant primary cell walls	388:411	plant primary cell walls	388:411	Taking a cue from nature, the build-up and composition of plant primary cell walls inspired the capsule wall assembly, because the primary cell walls in plants exhibit high mechanical properties despite being in a highly hydrated state, primarily owing to cellulose microfibrils.
28323423	0	36	theme	Cellulose	50:58	arg1	Nanofibers					60:69	Cellulose Nanofibers	50:69	Cellulose Nanofibers with Switchable Permeability	50:98	Bioinspired Layer-by-Layer Microcapsules Based on Cellulose Nanofibers with Switchable Permeability.
28323423	5	37	theme	larger	999:1004	arg1	molecules					1006:1014	larger molecules	999:1014	larger molecules (∼12 nm)	999:1023	Upon exposure to NaCl, the porosity of the capsule wall quickly changed allowing larger molecules (∼12 nm) to permeate.
28323423	5	37	theme	larger	999:1004	arg1	nm					1021:1022	∼12 nm	1017:1022	∼12 nm	1017:1022	Upon exposure to NaCl, the porosity of the capsule wall quickly changed allowing larger molecules (∼12 nm) to permeate.
28323423	2	38	theme	walls	407:411	arg1	composition					373:383	composition	373:383	composition	373:383	Taking a cue from nature, the build-up and composition of plant primary cell walls inspired the capsule wall assembly, because the primary cell walls in plants exhibit high mechanical properties despite being in a highly hydrated state, primarily owing to cellulose microfibrils.
28323423	2	38	theme	walls	407:411	arg1	build-up					360:367	build-up	360:367	build-up	360:367	Taking a cue from nature, the build-up and composition of plant primary cell walls inspired the capsule wall assembly, because the primary cell walls in plants exhibit high mechanical properties despite being in a highly hydrated state, primarily owing to cellulose microfibrils.
28323423	2	39	theme	capsule	426:432	arg1	assembly					439:446	the capsule wall assembly	422:446	the capsule wall assembly	422:446	Taking a cue from nature, the build-up and composition of plant primary cell walls inspired the capsule wall assembly, because the primary cell walls in plants exhibit high mechanical properties despite being in a highly hydrated state, primarily owing to cellulose microfibrils.
28323423	2	40	theme	wall	434:437	arg1	assembly					439:446	the capsule wall assembly	422:446	the capsule wall assembly	422:446	Taking a cue from nature, the build-up and composition of plant primary cell walls inspired the capsule wall assembly, because the primary cell walls in plants exhibit high mechanical properties despite being in a highly hydrated state, primarily owing to cellulose microfibrils.
28323423	7	41	with	times	1363:1367	arg1	capsule					1383:1389	the same capsule	1374:1389	the same capsule	1374:1389	The high integrity of cell wall was due to the CNF and the ON/OFF alteration of the permeability properties, and subsequent loading/unloading of molecules, could be repeated several times with the same capsule demonstrating a robust microcontainer with controllable permeability properties.
28323423	4	42	theme	capsule	830:836	arg1	permeable					847:855	permeable	847:855	permeable	847:855	In water, the capsule wall was permeable to labeled dextrans with a hydrodynamic diameter of ∼6.6 nm.
28323423	4	42	theme	capsule	830:836	arg1	wall					838:841	the capsule wall	826:841	the capsule wall	826:841	In water, the capsule wall was permeable to labeled dextrans with a hydrodynamic diameter of ∼6.6 nm.
28323423	2	43	theme	cellulose	586:594	arg1	microfibrils					596:607	cellulose microfibrils	586:607	cellulose microfibrils	586:607	Taking a cue from nature, the build-up and composition of plant primary cell walls inspired the capsule wall assembly, because the primary cell walls in plants exhibit high mechanical properties despite being in a highly hydrated state, primarily owing to cellulose microfibrils.
28323423	3	44	theme	sacrificial	706:716	arg1	templates					724:732	sacrificial CaCO3 templates	706:732	sacrificial CaCO3 templates	706:732	The microcapsules (16 ± 4 μm in diameter) were fabricated using the layer-by-layer technique on sacrificial CaCO3 templates, using plant polysaccharides (pectin, cellulose nanofibers, and xyloglucan) only.
28323423	4	45	theme	hydrodynamic	884:895	arg1	diameter					897:904	a hydrodynamic diameter	882:904	a hydrodynamic diameter of ∼6.6 nm	882:915	In water, the capsule wall was permeable to labeled dextrans with a hydrodynamic diameter of ∼6.6 nm.
28323423	3	46	theme	layer-by-layer	678:691	arg1	technique					693:701	the layer-by-layer technique	674:701	the layer-by-layer technique on sacrificial CaCO3 templates	674:732	The microcapsules (16 ± 4 μm in diameter) were fabricated using the layer-by-layer technique on sacrificial CaCO3 templates, using plant polysaccharides (pectin, cellulose nanofibers, and xyloglucan) only.
28323423	7	47	theme	subsequent	1294:1303	arg1	loading/unloading					1305:1321	subsequent loading/unloading	1294:1321	subsequent loading/unloading of molecules	1294:1334	The high integrity of cell wall was due to the CNF and the ON/OFF alteration of the permeability properties, and subsequent loading/unloading of molecules, could be repeated several times with the same capsule demonstrating a robust microcontainer with controllable permeability properties.
28323423	6	48	theme	original	1085:1092	arg1	state					1094:1098	its original state	1081:1098	its original state	1081:1098	However, the porosity could be restored to its original state by removal of NaCl, by which permeants became trapped inside the capsule's core.
28323423	7	49	theme	robust	1407:1412	arg1	microcontainer					1414:1427	a robust microcontainer	1405:1427	a robust microcontainer with controllable permeability properties	1405:1469	The high integrity of cell wall was due to the CNF and the ON/OFF alteration of the permeability properties, and subsequent loading/unloading of molecules, could be repeated several times with the same capsule demonstrating a robust microcontainer with controllable permeability properties.
28323423	3	50	dep	polysaccharides	747:761	arg1	polysaccharides					747:761	plant polysaccharides	741:761	plant polysaccharides (pectin, cellulose nanofibers, and xyloglucan)	741:808	The microcapsules (16 ± 4 μm in diameter) were fabricated using the layer-by-layer technique on sacrificial CaCO3 templates, using plant polysaccharides (pectin, cellulose nanofibers, and xyloglucan) only.
28323423	3	50	dep	polysaccharides	747:761	arg1	pectin					764:769	pectin	764:769	pectin	764:769	The microcapsules (16 ± 4 μm in diameter) were fabricated using the layer-by-layer technique on sacrificial CaCO3 templates, using plant polysaccharides (pectin, cellulose nanofibers, and xyloglucan) only.
28323423	3	50	dep	polysaccharides	747:761	arg1	nanofibers					782:791	cellulose nanofibers	772:791	cellulose nanofibers	772:791	The microcapsules (16 ± 4 μm in diameter) were fabricated using the layer-by-layer technique on sacrificial CaCO3 templates, using plant polysaccharides (pectin, cellulose nanofibers, and xyloglucan) only.
28323423	3	50	dep	polysaccharides	747:761	arg1	xyloglucan					798:807	xyloglucan	798:807	xyloglucan	798:807	The microcapsules (16 ± 4 μm in diameter) were fabricated using the layer-by-layer technique on sacrificial CaCO3 templates, using plant polysaccharides (pectin, cellulose nanofibers, and xyloglucan) only.
28323423	1	51	from	promise	263:269	arg1	applications					274:285	applications	274:285	applications based on selective and timed permeability	274:327	Green, all-polysaccharide based microcapsules with mechanically robust capsule walls and fast, stimuli-triggered, and switchable permeability behavior show great promise in applications based on selective and timed permeability.
28323423	7	52	theme	properties	1278:1287	arg1	loading/unloading					1305:1321	subsequent loading/unloading	1294:1321	subsequent loading/unloading of molecules	1294:1334	The high integrity of cell wall was due to the CNF and the ON/OFF alteration of the permeability properties, and subsequent loading/unloading of molecules, could be repeated several times with the same capsule demonstrating a robust microcontainer with controllable permeability properties.
28323423	7	52	theme	properties	1278:1287	arg1	alteration					1247:1256	the ON/OFF alteration	1236:1256	the ON/OFF alteration of the permeability properties	1236:1287	The high integrity of cell wall was due to the CNF and the ON/OFF alteration of the permeability properties, and subsequent loading/unloading of molecules, could be repeated several times with the same capsule demonstrating a robust microcontainer with controllable permeability properties.
28323423	5	53	theme	wall	969:972	arg1	porosity					945:952	the porosity	941:952	the porosity of the capsule wall	941:972	Upon exposure to NaCl, the porosity of the capsule wall quickly changed allowing larger molecules (∼12 nm) to permeate.
28323423	4	54	from	water	819:823	arg1	permeable					847:855	permeable	847:855	permeable	847:855	In water, the capsule wall was permeable to labeled dextrans with a hydrodynamic diameter of ∼6.6 nm.
28323423	4	54	from	water	819:823	arg1	wall					838:841	the capsule wall	826:841	the capsule wall	826:841	In water, the capsule wall was permeable to labeled dextrans with a hydrodynamic diameter of ∼6.6 nm.
28323423	1	55	theme	robust	165:170	arg1	walls					180:184	mechanically robust capsule walls	152:184	mechanically robust capsule walls	152:184	Green, all-polysaccharide based microcapsules with mechanically robust capsule walls and fast, stimuli-triggered, and switchable permeability behavior show great promise in applications based on selective and timed permeability.
28323423	7	56	theme	molecules	1326:1334	arg1	loading/unloading					1305:1321	subsequent loading/unloading	1294:1321	subsequent loading/unloading of molecules	1294:1334	The high integrity of cell wall was due to the CNF and the ON/OFF alteration of the permeability properties, and subsequent loading/unloading of molecules, could be repeated several times with the same capsule demonstrating a robust microcontainer with controllable permeability properties.
28323423	7	56	theme	molecules	1326:1334	arg1	alteration					1247:1256	the ON/OFF alteration	1236:1256	the ON/OFF alteration of the permeability properties	1236:1287	The high integrity of cell wall was due to the CNF and the ON/OFF alteration of the permeability properties, and subsequent loading/unloading of molecules, could be repeated several times with the same capsule demonstrating a robust microcontainer with controllable permeability properties.
28323423	5	57	theme	capsule	961:967	arg1	wall					969:972	the capsule wall	957:972	the capsule wall	957:972	Upon exposure to NaCl, the porosity of the capsule wall quickly changed allowing larger molecules (∼12 nm) to permeate.
28323423	7	58	theme	controllable	1434:1445	arg1	properties					1460:1469	controllable permeability properties	1434:1469	controllable permeability properties	1434:1469	The high integrity of cell wall was due to the CNF and the ON/OFF alteration of the permeability properties, and subsequent loading/unloading of molecules, could be repeated several times with the same capsule demonstrating a robust microcontainer with controllable permeability properties.
28323423	1	59	with	microcapsules	133:145	arg1	behavior					243:250	fast, stimuli-triggered, and switchable permeability behavior	190:250	fast, stimuli-triggered, and switchable permeability behavior	190:250	Green, all-polysaccharide based microcapsules with mechanically robust capsule walls and fast, stimuli-triggered, and switchable permeability behavior show great promise in applications based on selective and timed permeability.
28323423	1	59	with	microcapsules	133:145	arg1	walls					180:184	mechanically robust capsule walls	152:184	mechanically robust capsule walls	152:184	Green, all-polysaccharide based microcapsules with mechanically robust capsule walls and fast, stimuli-triggered, and switchable permeability behavior show great promise in applications based on selective and timed permeability.
28323423	1	60	theme	capsule	172:178	arg1	walls					180:184	mechanically robust capsule walls	152:184	mechanically robust capsule walls	152:184	Green, all-polysaccharide based microcapsules with mechanically robust capsule walls and fast, stimuli-triggered, and switchable permeability behavior show great promise in applications based on selective and timed permeability.
28323423	4	61	with	dextrans	868:875	arg1	diameter					897:904	a hydrodynamic diameter	882:904	a hydrodynamic diameter of ∼6.6 nm	882:915	In water, the capsule wall was permeable to labeled dextrans with a hydrodynamic diameter of ∼6.6 nm.
28323423	7	62	theme	cell	1203:1206	arg1	wall					1208:1211	cell wall	1203:1211	cell wall	1203:1211	The high integrity of cell wall was due to the CNF and the ON/OFF alteration of the permeability properties, and subsequent loading/unloading of molecules, could be repeated several times with the same capsule demonstrating a robust microcontainer with controllable permeability properties.
28323423	1	63	theme	great	257:261	arg1	promise					263:269	great promise	257:269	great promise in applications based on selective and timed permeability	257:327	Green, all-polysaccharide based microcapsules with mechanically robust capsule walls and fast, stimuli-triggered, and switchable permeability behavior show great promise in applications based on selective and timed permeability.
28323423	3	64	theme	±	632:632	arg1	μm					636:637	16 ± 4 μm	629:637	16 ± 4 μm in diameter	629:649	The microcapsules (16 ± 4 μm in diameter) were fabricated using the layer-by-layer technique on sacrificial CaCO3 templates, using plant polysaccharides (pectin, cellulose nanofibers, and xyloglucan) only.
28323423	0	65	with	Nanofibers	60:69	arg1	Permeability					87:98	Switchable Permeability	76:98	Switchable Permeability	76:98	Bioinspired Layer-by-Layer Microcapsules Based on Cellulose Nanofibers with Switchable Permeability.
28323423	2	66	theme	plant	388:392	arg1	walls					407:411	plant primary cell walls	388:411	plant primary cell walls	388:411	Taking a cue from nature, the build-up and composition of plant primary cell walls inspired the capsule wall assembly, because the primary cell walls in plants exhibit high mechanical properties despite being in a highly hydrated state, primarily owing to cellulose microfibrils.
28323423	0	67	theme	Switchable	76:85	arg1	Permeability					87:98	Switchable Permeability	76:98	Switchable Permeability	76:98	Bioinspired Layer-by-Layer Microcapsules Based on Cellulose Nanofibers with Switchable Permeability.
28323423	3	68	theme	CaCO3	718:722	arg1	templates					724:732	sacrificial CaCO3 templates	706:732	sacrificial CaCO3 templates	706:732	The microcapsules (16 ± 4 μm in diameter) were fabricated using the layer-by-layer technique on sacrificial CaCO3 templates, using plant polysaccharides (pectin, cellulose nanofibers, and xyloglucan) only.
28323423	7	69	theme	permeability	1265:1276	arg1	properties					1278:1287	the permeability properties	1261:1287	the permeability properties	1261:1287	The high integrity of cell wall was due to the CNF and the ON/OFF alteration of the permeability properties, and subsequent loading/unloading of molecules, could be repeated several times with the same capsule demonstrating a robust microcontainer with controllable permeability properties.
28323423	2	70	theme	primary	394:400	arg1	walls					407:411	plant primary cell walls	388:411	plant primary cell walls	388:411	Taking a cue from nature, the build-up and composition of plant primary cell walls inspired the capsule wall assembly, because the primary cell walls in plants exhibit high mechanical properties despite being in a highly hydrated state, primarily owing to cellulose microfibrils.
27554431	7	0	theme	distinct	1066:1073	arg1	changes					1075:1081	distinct changes	1066:1081	distinct changes in the Lefty A/Lefty B gene(s), and protein expression	1066:1136	Differentiation of hESCs to mesenchymal cells (MSCs) or neuronal progenitor cells (NPCs) entails distinct changes in the Lefty A/Lefty B gene(s), and protein expression.
27554431	1	1	theme	pathway	269:275	arg1	antagonist					221:230	a potent antagonist	212:230	a potent antagonist of the TGF-β/Nodal/Activin signaling pathway	212:275	Lefty is a member of transforming growth factor-beta (TGF-β) superfamily and a potent antagonist of the TGF-β/Nodal/Activin signaling pathway.
27554431	1	1	theme	pathway	269:275	arg1	Lefty					135:139	Lefty	135:139	Lefty	135:139	Lefty is a member of transforming growth factor-beta (TGF-β) superfamily and a potent antagonist of the TGF-β/Nodal/Activin signaling pathway.
27554431	1	1	theme	pathway	269:275	arg1	member					146:151	a member	144:151	a member of transforming growth factor-beta (TGF-β) superfamily	144:206	Lefty is a member of transforming growth factor-beta (TGF-β) superfamily and a potent antagonist of the TGF-β/Nodal/Activin signaling pathway.
27554431	9	2	theme	melanoma	1373:1380	arg1	cells					1382:1386	aggressive melanoma cells	1362:1386	aggressive melanoma cells	1362:1386	These modifications affected Lefty's inhibitory effect on Nodal signaling in aggressive melanoma cells.
27554431	4	3	theme	"	645:645	arg1	moiety					647:652	their "glycan" moiety	632:652	their "glycan" moiety	632:652	Lefty proteins (human Lefty A and B) are secreted glycoproteins, but their mode of secretion and the significance of their "glycan" moiety remain mostly unexplored.
27554431	5	4	theme	extracellular	797:809	arg1	release					811:817	extracellular release	797:817	extracellular release	797:817	By employing an in vitro system of human ESCs (hESCs), we observed that Lefty protein(s) are encased in exosomes for extracellular release.
27554431	7	5	theme	progenitor	1034:1043	arg1	cells					1045:1049	neuronal progenitor cells	1025:1049	neuronal progenitor cells (NPCs)	1025:1056	Differentiation of hESCs to mesenchymal cells (MSCs) or neuronal progenitor cells (NPCs) entails distinct changes in the Lefty A/Lefty B gene(s), and protein expression.
27554431	7	5	theme	progenitor	1034:1043	arg1	NPCs					1052:1055	NPCs	1052:1055	NPCs	1052:1055	Differentiation of hESCs to mesenchymal cells (MSCs) or neuronal progenitor cells (NPCs) entails distinct changes in the Lefty A/Lefty B gene(s), and protein expression.
27554431	8	6	theme	N-glycan	1182:1189	arg1	composition					1191:1201	N-glycan composition	1182:1201	N-glycan composition	1182:1201	Specifically, the proteolytic cleavage and N-glycan composition of the cell-associated and exosomal Lefty differ in the differentiated progenies.
27554431	10	7	theme	efficient	1512:1520	arg1	means					1522:1526	efficient means	1512:1526	efficient means of diversifying the endogenous functions of Lefty	1512:1576	The microheterogeneity in the processing and glycosylation of Lefty protein(s) between hESCs, MSCs, and NPCs could present efficient means of diversifying the endogenous functions of Lefty.
27554431	2	8	theme	self-renewal/pluripotency	310:334	arg1	status					336:341	self-renewal/pluripotency status	310:341	self-renewal/pluripotency status	310:341	Lefty is critical in sustaining self-renewal/pluripotency status, and implicated in the differentiation of embryonic stem cells (ESCs).
27554431	0	9	from	Route	74:78	arg1	Differentiation					118:132	Differentiation	118:132	Differentiation	118:132	Lefty Glycoproteins in Human Embryonic Stem Cells: Extracellular Delivery Route and Posttranslational Modification in Differentiation.
27554431	7	10	theme	A/Lefty	1096:1102	arg1	s					1111:1111	s	1111:1111	s	1111:1111	Differentiation of hESCs to mesenchymal cells (MSCs) or neuronal progenitor cells (NPCs) entails distinct changes in the Lefty A/Lefty B gene(s), and protein expression.
27554431	7	10	theme	A/Lefty	1096:1102	arg1	gene					1106:1109	the Lefty A/Lefty B gene	1086:1109	the Lefty A/Lefty B gene(s)	1086:1112	Differentiation of hESCs to mesenchymal cells (MSCs) or neuronal progenitor cells (NPCs) entails distinct changes in the Lefty A/Lefty B gene(s), and protein expression.
27554431	1	11	theme	potent	214:219	arg1	antagonist					221:230	a potent antagonist	212:230	a potent antagonist of the TGF-β/Nodal/Activin signaling pathway	212:275	Lefty is a member of transforming growth factor-beta (TGF-β) superfamily and a potent antagonist of the TGF-β/Nodal/Activin signaling pathway.
27554431	11	12	from	range	1663:1667	arg1	patterning					1626:1635	embryonic patterning	1616:1635	embryonic patterning	1616:1635	Whether Lefty's diverse functions in embryonic patterning, as well as its diffusion range in the extracellular environment, are similarly affected remains to be determined.
27554431	11	12	from	range	1663:1667	arg1	environment					1690:1700	the extracellular environment	1672:1700	the extracellular environment	1672:1700	Whether Lefty's diverse functions in embryonic patterning, as well as its diffusion range in the extracellular environment, are similarly affected remains to be determined.
27554431	8	13	theme	cell-associated	1210:1224	arg1	Lefty					1239:1243	the cell-associated and exosomal Lefty	1206:1243	Lefty	1239:1243	Specifically, the proteolytic cleavage and N-glycan composition of the cell-associated and exosomal Lefty differ in the differentiated progenies.
27554431	6	14	theme	proteolytic	877:887	arg1	processing					889:898	their proteolytic processing	871:898	their proteolytic processing	871:898	The exosomal- and cell-associated Lefty diverge in their proteolytic processing, and possess N-glycan structures of high mannose and complex nature.
27554431	8	15	theme	exosomal	1230:1237	arg1	Lefty					1239:1243	the cell-associated and exosomal Lefty	1206:1243	Lefty	1239:1243	Specifically, the proteolytic cleavage and N-glycan composition of the cell-associated and exosomal Lefty differ in the differentiated progenies.
27554431	5	16	dep	in	696:697	arg1	vitro					699:703	vitro	699:703	vitro	699:703	By employing an in vitro system of human ESCs (hESCs), we observed that Lefty protein(s) are encased in exosomes for extracellular release.
27554431	0	17	theme	Extracellular	51:63	arg1	Route					74:78	Extracellular Delivery Route	51:78	Extracellular Delivery Route	51:78	Lefty Glycoproteins in Human Embryonic Stem Cells: Extracellular Delivery Route and Posttranslational Modification in Differentiation.
27554431	0	17	theme	Extracellular	51:63	arg1	Glycoproteins					6:18	Lefty Glycoproteins	0:18	Lefty Glycoproteins in Human Embryonic Stem Cells: Extracellular Delivery Route and Posttranslational Modification in Differentiation.	0:133	Lefty Glycoproteins in Human Embryonic Stem Cells: Extracellular Delivery Route and Posttranslational Modification in Differentiation.
27554431	10	18	dep	processing	1419:1428	arg1	the					1415:1417	the	1415:1417	the	1415:1417	The microheterogeneity in the processing and glycosylation of Lefty protein(s) between hESCs, MSCs, and NPCs could present efficient means of diversifying the endogenous functions of Lefty.
27554431	6	19	theme	nature	961:966	arg1	structures					922:931	N-glycan structures	913:931	N-glycan structures of high mannose and complex nature	913:966	The exosomal- and cell-associated Lefty diverge in their proteolytic processing, and possess N-glycan structures of high mannose and complex nature.
27554431	4	20	theme	Lefty	515:519	arg1	proteins					521:528	Lefty proteins	515:528	Lefty proteins (human Lefty A and B)	515:550	Lefty proteins (human Lefty A and B) are secreted glycoproteins, but their mode of secretion and the significance of their "glycan" moiety remain mostly unexplored.
27554431	4	20	theme	Lefty	515:519	arg1	B					549:549	B	549:549	B	549:549	Lefty proteins (human Lefty A and B) are secreted glycoproteins, but their mode of secretion and the significance of their "glycan" moiety remain mostly unexplored.
27554431	4	20	theme	Lefty	515:519	arg1	A					543:543	human Lefty A	531:543	human Lefty A	531:543	Lefty proteins (human Lefty A and B) are secreted glycoproteins, but their mode of secretion and the significance of their "glycan" moiety remain mostly unexplored.
27554431	4	20	theme	Lefty	515:519	arg1	glycoproteins					565:577	secreted glycoproteins	556:577	secreted glycoproteins	556:577	Lefty proteins (human Lefty A and B) are secreted glycoproteins, but their mode of secretion and the significance of their "glycan" moiety remain mostly unexplored.
27554431	1	21	theme	growth	169:174	arg1	TGF-β					189:193	TGF-β	189:193	TGF-β	189:193	Lefty is a member of transforming growth factor-beta (TGF-β) superfamily and a potent antagonist of the TGF-β/Nodal/Activin signaling pathway.
27554431	1	21	theme	growth	169:174	arg1	factor-beta					176:186	transforming growth factor-beta	156:186	transforming growth factor-beta (TGF-β) superfamily	156:206	Lefty is a member of transforming growth factor-beta (TGF-β) superfamily and a potent antagonist of the TGF-β/Nodal/Activin signaling pathway.
27554431	0	22	theme	Posttranslational	84:100	arg1	Modification					102:113	Posttranslational Modification	84:113	Posttranslational Modification	84:113	Lefty Glycoproteins in Human Embryonic Stem Cells: Extracellular Delivery Route and Posttranslational Modification in Differentiation.
27554431	0	22	theme	Posttranslational	84:100	arg1	Glycoproteins					6:18	Lefty Glycoproteins	0:18	Lefty Glycoproteins in Human Embryonic Stem Cells: Extracellular Delivery Route and Posttranslational Modification in Differentiation.	0:133	Lefty Glycoproteins in Human Embryonic Stem Cells: Extracellular Delivery Route and Posttranslational Modification in Differentiation.
27554431	6	23	theme	high	936:939	arg1	mannose					941:947	high mannose	936:947	high mannose	936:947	The exosomal- and cell-associated Lefty diverge in their proteolytic processing, and possess N-glycan structures of high mannose and complex nature.
27554431	5	24	theme	ESCs	721:724	arg1	system					705:710	an in vitro system	693:710	an in vitro system of human ESCs (hESCs)	693:732	By employing an in vitro system of human ESCs (hESCs), we observed that Lefty protein(s) are encased in exosomes for extracellular release.
27554431	0	25	theme	Lefty	0:4	arg1	Route					74:78	Extracellular Delivery Route	51:78	Extracellular Delivery Route	51:78	Lefty Glycoproteins in Human Embryonic Stem Cells: Extracellular Delivery Route and Posttranslational Modification in Differentiation.
27554431	0	25	theme	Lefty	0:4	arg1	Modification					102:113	Posttranslational Modification	84:113	Posttranslational Modification	84:113	Lefty Glycoproteins in Human Embryonic Stem Cells: Extracellular Delivery Route and Posttranslational Modification in Differentiation.
27554431	0	25	theme	Lefty	0:4	arg1	Glycoproteins					6:18	Lefty Glycoproteins	0:18	Lefty Glycoproteins in Human Embryonic Stem Cells: Extracellular Delivery Route and Posttranslational Modification in Differentiation.	0:133	Lefty Glycoproteins in Human Embryonic Stem Cells: Extracellular Delivery Route and Posttranslational Modification in Differentiation.
27554431	11	26	from	functions	1603:1611	arg1	patterning					1626:1635	embryonic patterning	1616:1635	embryonic patterning	1616:1635	Whether Lefty's diverse functions in embryonic patterning, as well as its diffusion range in the extracellular environment, are similarly affected remains to be determined.
27554431	11	26	from	functions	1603:1611	arg1	environment					1690:1700	the extracellular environment	1672:1700	the extracellular environment	1672:1700	Whether Lefty's diverse functions in embryonic patterning, as well as its diffusion range in the extracellular environment, are similarly affected remains to be determined.
27554431	10	27	theme	protein	1457:1463	arg1	s					1465:1465	Lefty protein(s)	1451:1466	Lefty protein(s) between hESCs, MSCs, and NPCs	1451:1496	The microheterogeneity in the processing and glycosylation of Lefty protein(s) between hESCs, MSCs, and NPCs could present efficient means of diversifying the endogenous functions of Lefty.
27554431	12	28	theme	exosomes	1817:1824	arg1	relevance					1789:1797	the potential relevance	1775:1797	the potential relevance of Lefty-packaged exosomes for combating debilitating diseases such as cancer	1775:1875	Our studies underscore the potential relevance of Lefty-packaged exosomes for combating debilitating diseases such as cancer.
27554431	7	29	theme	B	1104:1104	arg1	s					1111:1111	s	1111:1111	s	1111:1111	Differentiation of hESCs to mesenchymal cells (MSCs) or neuronal progenitor cells (NPCs) entails distinct changes in the Lefty A/Lefty B gene(s), and protein expression.
27554431	7	29	theme	B	1104:1104	arg1	gene					1106:1109	the Lefty A/Lefty B gene	1086:1109	the Lefty A/Lefty B gene(s)	1086:1112	Differentiation of hESCs to mesenchymal cells (MSCs) or neuronal progenitor cells (NPCs) entails distinct changes in the Lefty A/Lefty B gene(s), and protein expression.
27554431	3	30	theme	emerging	423:430	arg1	studies					432:438	emerging studies	423:438	emerging studies	423:438	However, emerging studies depict Lefty as a multifaceted protein involved in myriad cellular events.
27554431	4	31	theme	secreted	556:563	arg1	proteins					521:528	Lefty proteins	515:528	Lefty proteins (human Lefty A and B)	515:550	Lefty proteins (human Lefty A and B) are secreted glycoproteins, but their mode of secretion and the significance of their "glycan" moiety remain mostly unexplored.
27554431	4	31	theme	secreted	556:563	arg1	glycoproteins					565:577	secreted glycoproteins	556:577	secreted glycoproteins	556:577	Lefty proteins (human Lefty A and B) are secreted glycoproteins, but their mode of secretion and the significance of their "glycan" moiety remain mostly unexplored.
27554431	6	32	contain	possess	905:911	arg2	structures					922:931	N-glycan structures	913:931	N-glycan structures of high mannose and complex nature	913:966	The exosomal- and cell-associated Lefty diverge in their proteolytic processing, and possess N-glycan structures of high mannose and complex nature.
27554431	6	32	contain	possess	905:911	arg1	Lefty					854:858	The exosomal- and cell-associated Lefty	820:858	The exosomal- and cell-associated Lefty	820:858	The exosomal- and cell-associated Lefty diverge in their proteolytic processing, and possess N-glycan structures of high mannose and complex nature.
27554431	3	33	theme	multifaceted	458:469	arg1	protein					471:477	a multifaceted protein	456:477	a multifaceted protein involved in myriad cellular events	456:512	However, emerging studies depict Lefty as a multifaceted protein involved in myriad cellular events.
27554431	3	33	theme	multifaceted	458:469	arg1	Lefty					447:451	Lefty	447:451	Lefty	447:451	However, emerging studies depict Lefty as a multifaceted protein involved in myriad cellular events.
27554431	0	34	theme	Embryonic	29:37	arg1	Cells					44:48	Human Embryonic Stem Cells	23:48	Human Embryonic Stem Cells	23:48	Lefty Glycoproteins in Human Embryonic Stem Cells: Extracellular Delivery Route and Posttranslational Modification in Differentiation.
27554431	7	35	theme	Lefty	1090:1094	arg1	s					1111:1111	s	1111:1111	s	1111:1111	Differentiation of hESCs to mesenchymal cells (MSCs) or neuronal progenitor cells (NPCs) entails distinct changes in the Lefty A/Lefty B gene(s), and protein expression.
27554431	7	35	theme	Lefty	1090:1094	arg1	gene					1106:1109	the Lefty A/Lefty B gene	1086:1109	the Lefty A/Lefty B gene(s)	1086:1112	Differentiation of hESCs to mesenchymal cells (MSCs) or neuronal progenitor cells (NPCs) entails distinct changes in the Lefty A/Lefty B gene(s), and protein expression.
27554431	4	36	theme	Lefty	537:541	arg1	proteins					521:528	Lefty proteins	515:528	Lefty proteins (human Lefty A and B)	515:550	Lefty proteins (human Lefty A and B) are secreted glycoproteins, but their mode of secretion and the significance of their "glycan" moiety remain mostly unexplored.
27554431	4	36	theme	Lefty	537:541	arg1	A					543:543	human Lefty A	531:543	human Lefty A	531:543	Lefty proteins (human Lefty A and B) are secreted glycoproteins, but their mode of secretion and the significance of their "glycan" moiety remain mostly unexplored.
27554431	5	37	theme	Lefty	752:756	arg1	s					766:766	Lefty protein(s)	752:767	Lefty protein(s)	752:767	By employing an in vitro system of human ESCs (hESCs), we observed that Lefty protein(s) are encased in exosomes for extracellular release.
27554431	4	38	dep	proteins	521:528	arg1	proteins					521:528	Lefty proteins	515:528	Lefty proteins (human Lefty A and B)	515:550	Lefty proteins (human Lefty A and B) are secreted glycoproteins, but their mode of secretion and the significance of their "glycan" moiety remain mostly unexplored.
27554431	4	38	dep	proteins	521:528	arg1	B					549:549	B	549:549	B	549:549	Lefty proteins (human Lefty A and B) are secreted glycoproteins, but their mode of secretion and the significance of their "glycan" moiety remain mostly unexplored.
27554431	4	38	dep	proteins	521:528	arg1	A					543:543	human Lefty A	531:543	human Lefty A	531:543	Lefty proteins (human Lefty A and B) are secreted glycoproteins, but their mode of secretion and the significance of their "glycan" moiety remain mostly unexplored.
27554431	10	39	theme	s	1465:1465	arg1	glycosylation					1434:1446	glycosylation	1434:1446	glycosylation	1434:1446	The microheterogeneity in the processing and glycosylation of Lefty protein(s) between hESCs, MSCs, and NPCs could present efficient means of diversifying the endogenous functions of Lefty.
27554431	10	39	theme	s	1465:1465	arg1	processing					1419:1428	processing	1419:1428	processing	1419:1428	The microheterogeneity in the processing and glycosylation of Lefty protein(s) between hESCs, MSCs, and NPCs could present efficient means of diversifying the endogenous functions of Lefty.
27554431	11	40	theme	diffusion	1653:1661	arg1	range					1663:1667	its diffusion range	1649:1667	its diffusion range in the extracellular environment	1649:1700	Whether Lefty's diverse functions in embryonic patterning, as well as its diffusion range in the extracellular environment, are similarly affected remains to be determined.
27554431	3	41	theme	myriad	491:496	arg1	events					507:512	myriad cellular events	491:512	myriad cellular events	491:512	However, emerging studies depict Lefty as a multifaceted protein involved in myriad cellular events.
27554431	9	42	theme	inhibitory	1322:1331	arg1	effect					1333:1338	Lefty's inhibitory effect	1314:1338	Lefty's inhibitory effect on Nodal signaling in aggressive melanoma cells	1314:1386	These modifications affected Lefty's inhibitory effect on Nodal signaling in aggressive melanoma cells.
27554431	9	43	from	effect	1333:1338	arg1	signaling					1349:1357	Nodal signaling	1343:1357	Nodal signaling in aggressive melanoma cells	1343:1386	These modifications affected Lefty's inhibitory effect on Nodal signaling in aggressive melanoma cells.
27554431	2	44	theme	cells	400:404	arg1	differentiation					366:380	the differentiation	362:380	the differentiation of embryonic stem cells (ESCs)	362:411	Lefty is critical in sustaining self-renewal/pluripotency status, and implicated in the differentiation of embryonic stem cells (ESCs).
27554431	2	45	theme	embryonic	385:393	arg1	ESCs					407:410	ESCs	407:410	ESCs	407:410	Lefty is critical in sustaining self-renewal/pluripotency status, and implicated in the differentiation of embryonic stem cells (ESCs).
27554431	2	45	theme	embryonic	385:393	arg1	cells					400:404	embryonic stem cells	385:404	embryonic stem cells (ESCs)	385:411	Lefty is critical in sustaining self-renewal/pluripotency status, and implicated in the differentiation of embryonic stem cells (ESCs).
27554431	1	46	theme	signaling	259:267	arg1	pathway					269:275	the TGF-β/Nodal/Activin signaling pathway	235:275	the TGF-β/Nodal/Activin signaling pathway	235:275	Lefty is a member of transforming growth factor-beta (TGF-β) superfamily and a potent antagonist of the TGF-β/Nodal/Activin signaling pathway.
27554431	9	47	theme	aggressive	1362:1371	arg1	cells					1382:1386	aggressive melanoma cells	1362:1386	aggressive melanoma cells	1362:1386	These modifications affected Lefty's inhibitory effect on Nodal signaling in aggressive melanoma cells.
27554431	6	48	theme	cell-associated	838:852	arg1	Lefty					854:858	The exosomal- and cell-associated Lefty	820:858	The exosomal- and cell-associated Lefty	820:858	The exosomal- and cell-associated Lefty diverge in their proteolytic processing, and possess N-glycan structures of high mannose and complex nature.
27554431	1	49	theme	factor-beta	176:186	arg1	superfamily					196:206	transforming growth factor-beta (TGF-β) superfamily	156:206	transforming growth factor-beta (TGF-β) superfamily	156:206	Lefty is a member of transforming growth factor-beta (TGF-β) superfamily and a potent antagonist of the TGF-β/Nodal/Activin signaling pathway.
27554431	6	50	theme	exosomal-	824:832	arg1	Lefty					854:858	The exosomal- and cell-associated Lefty	820:858	The exosomal- and cell-associated Lefty	820:858	The exosomal- and cell-associated Lefty diverge in their proteolytic processing, and possess N-glycan structures of high mannose and complex nature.
27554431	4	51	theme	glycan	639:644	arg1	moiety					647:652	their "glycan" moiety	632:652	their "glycan" moiety	632:652	Lefty proteins (human Lefty A and B) are secreted glycoproteins, but their mode of secretion and the significance of their "glycan" moiety remain mostly unexplored.
27554431	11	52	theme	diverse	1595:1601	arg1	functions					1603:1611	Lefty's diverse functions	1587:1611	Lefty's diverse functions in embryonic patterning	1587:1635	Whether Lefty's diverse functions in embryonic patterning, as well as its diffusion range in the extracellular environment, are similarly affected remains to be determined.
27554431	7	53	theme	neuronal	1025:1032	arg1	cells					1045:1049	neuronal progenitor cells	1025:1049	neuronal progenitor cells (NPCs)	1025:1056	Differentiation of hESCs to mesenchymal cells (MSCs) or neuronal progenitor cells (NPCs) entails distinct changes in the Lefty A/Lefty B gene(s), and protein expression.
27554431	7	53	theme	neuronal	1025:1032	arg1	NPCs					1052:1055	NPCs	1052:1055	NPCs	1052:1055	Differentiation of hESCs to mesenchymal cells (MSCs) or neuronal progenitor cells (NPCs) entails distinct changes in the Lefty A/Lefty B gene(s), and protein expression.
27554431	4	54	theme	human	531:535	arg1	proteins					521:528	Lefty proteins	515:528	Lefty proteins (human Lefty A and B)	515:550	Lefty proteins (human Lefty A and B) are secreted glycoproteins, but their mode of secretion and the significance of their "glycan" moiety remain mostly unexplored.
27554431	4	54	theme	human	531:535	arg1	A					543:543	human Lefty A	531:543	human Lefty A	531:543	Lefty proteins (human Lefty A and B) are secreted glycoproteins, but their mode of secretion and the significance of their "glycan" moiety remain mostly unexplored.
27554431	4	55	theme	moiety	647:652	arg1	significance					616:627	the significance	612:627	the significance of their "glycan" moiety	612:652	Lefty proteins (human Lefty A and B) are secreted glycoproteins, but their mode of secretion and the significance of their "glycan" moiety remain mostly unexplored.
27554431	4	55	theme	moiety	647:652	arg1	mode					590:593	their mode	584:593	their mode of secretion	584:606	Lefty proteins (human Lefty A and B) are secreted glycoproteins, but their mode of secretion and the significance of their "glycan" moiety remain mostly unexplored.
27554431	1	56	theme	superfamily	196:206	arg1	antagonist					221:230	a potent antagonist	212:230	a potent antagonist of the TGF-β/Nodal/Activin signaling pathway	212:275	Lefty is a member of transforming growth factor-beta (TGF-β) superfamily and a potent antagonist of the TGF-β/Nodal/Activin signaling pathway.
27554431	1	56	theme	superfamily	196:206	arg1	Lefty					135:139	Lefty	135:139	Lefty	135:139	Lefty is a member of transforming growth factor-beta (TGF-β) superfamily and a potent antagonist of the TGF-β/Nodal/Activin signaling pathway.
27554431	1	56	theme	superfamily	196:206	arg1	member					146:151	a member	144:151	a member of transforming growth factor-beta (TGF-β) superfamily	144:206	Lefty is a member of transforming growth factor-beta (TGF-β) superfamily and a potent antagonist of the TGF-β/Nodal/Activin signaling pathway.
27554431	10	57	from	microheterogeneity	1393:1410	arg1	glycosylation					1434:1446	glycosylation	1434:1446	glycosylation	1434:1446	The microheterogeneity in the processing and glycosylation of Lefty protein(s) between hESCs, MSCs, and NPCs could present efficient means of diversifying the endogenous functions of Lefty.
27554431	10	57	from	microheterogeneity	1393:1410	arg1	processing					1419:1428	processing	1419:1428	processing	1419:1428	The microheterogeneity in the processing and glycosylation of Lefty protein(s) between hESCs, MSCs, and NPCs could present efficient means of diversifying the endogenous functions of Lefty.
27554431	10	58	gly	glycosylation	1434:1446	arg1	s					1465:1465	Lefty protein(s)	1451:1466	Lefty protein(s) between hESCs, MSCs, and NPCs	1451:1496	The microheterogeneity in the processing and glycosylation of Lefty protein(s) between hESCs, MSCs, and NPCs could present efficient means of diversifying the endogenous functions of Lefty.
27554431	5	59	theme	in	696:697	arg1	system					705:710	an in vitro system	693:710	an in vitro system of human ESCs (hESCs)	693:732	By employing an in vitro system of human ESCs (hESCs), we observed that Lefty protein(s) are encased in exosomes for extracellular release.
27554431	8	60	theme	proteolytic	1157:1167	arg1	cleavage					1169:1176	proteolytic cleavage	1157:1176	proteolytic cleavage	1157:1176	Specifically, the proteolytic cleavage and N-glycan composition of the cell-associated and exosomal Lefty differ in the differentiated progenies.
27554431	10	61	theme	endogenous	1548:1557	arg1	functions					1559:1567	the endogenous functions	1544:1567	the endogenous functions of Lefty	1544:1576	The microheterogeneity in the processing and glycosylation of Lefty protein(s) between hESCs, MSCs, and NPCs could present efficient means of diversifying the endogenous functions of Lefty.
27554431	7	62	theme	mesenchymal	997:1007	arg1	MSCs					1016:1019	MSCs	1016:1019	MSCs	1016:1019	Differentiation of hESCs to mesenchymal cells (MSCs) or neuronal progenitor cells (NPCs) entails distinct changes in the Lefty A/Lefty B gene(s), and protein expression.
27554431	7	62	theme	mesenchymal	997:1007	arg1	cells					1009:1013	mesenchymal cells	997:1013	mesenchymal cells (MSCs)	997:1020	Differentiation of hESCs to mesenchymal cells (MSCs) or neuronal progenitor cells (NPCs) entails distinct changes in the Lefty A/Lefty B gene(s), and protein expression.
27554431	7	63	theme	hESCs	988:992	arg1	Differentiation					969:983	Differentiation	969:983	Differentiation of hESCs to mesenchymal cells (MSCs) or neuronal progenitor cells (NPCs)	969:1056	Differentiation of hESCs to mesenchymal cells (MSCs) or neuronal progenitor cells (NPCs) entails distinct changes in the Lefty A/Lefty B gene(s), and protein expression.
27554431	4	64	gly	glycoproteins	565:577	arg1	proteins					521:528	Lefty proteins	515:528	Lefty proteins (human Lefty A and B)	515:550	Lefty proteins (human Lefty A and B) are secreted glycoproteins, but their mode of secretion and the significance of their "glycan" moiety remain mostly unexplored.
27554431	4	64	gly	glycoproteins	565:577	arg1	glycoproteins					565:577	secreted glycoproteins	556:577	secreted glycoproteins	556:577	Lefty proteins (human Lefty A and B) are secreted glycoproteins, but their mode of secretion and the significance of their "glycan" moiety remain mostly unexplored.
27554431	0	65	from	Modification	102:113	arg1	Differentiation					118:132	Differentiation	118:132	Differentiation	118:132	Lefty Glycoproteins in Human Embryonic Stem Cells: Extracellular Delivery Route and Posttranslational Modification in Differentiation.
27554431	11	66	theme	extracellular	1676:1688	arg1	environment					1690:1700	the extracellular environment	1672:1700	the extracellular environment	1672:1700	Whether Lefty's diverse functions in embryonic patterning, as well as its diffusion range in the extracellular environment, are similarly affected remains to be determined.
27554431	0	67	theme	Delivery	65:72	arg1	Route					74:78	Extracellular Delivery Route	51:78	Extracellular Delivery Route	51:78	Lefty Glycoproteins in Human Embryonic Stem Cells: Extracellular Delivery Route and Posttranslational Modification in Differentiation.
27554431	0	67	theme	Delivery	65:72	arg1	Glycoproteins					6:18	Lefty Glycoproteins	0:18	Lefty Glycoproteins in Human Embryonic Stem Cells: Extracellular Delivery Route and Posttranslational Modification in Differentiation.	0:133	Lefty Glycoproteins in Human Embryonic Stem Cells: Extracellular Delivery Route and Posttranslational Modification in Differentiation.
27554431	7	68	from	changes	1075:1081	arg1	expression					1127:1136	protein expression	1119:1136	protein expression	1119:1136	Differentiation of hESCs to mesenchymal cells (MSCs) or neuronal progenitor cells (NPCs) entails distinct changes in the Lefty A/Lefty B gene(s), and protein expression.
27554431	7	68	from	changes	1075:1081	arg1	s					1111:1111	s	1111:1111	s	1111:1111	Differentiation of hESCs to mesenchymal cells (MSCs) or neuronal progenitor cells (NPCs) entails distinct changes in the Lefty A/Lefty B gene(s), and protein expression.
27554431	7	68	from	changes	1075:1081	arg1	gene					1106:1109	the Lefty A/Lefty B gene	1086:1109	the Lefty A/Lefty B gene(s)	1086:1112	Differentiation of hESCs to mesenchymal cells (MSCs) or neuronal progenitor cells (NPCs) entails distinct changes in the Lefty A/Lefty B gene(s), and protein expression.
27554431	8	69	theme	Lefty	1239:1243	arg1	cleavage					1169:1176	proteolytic cleavage	1157:1176	proteolytic cleavage	1157:1176	Specifically, the proteolytic cleavage and N-glycan composition of the cell-associated and exosomal Lefty differ in the differentiated progenies.
27554431	8	69	theme	Lefty	1239:1243	arg1	composition					1191:1201	N-glycan composition	1182:1201	N-glycan composition	1182:1201	Specifically, the proteolytic cleavage and N-glycan composition of the cell-associated and exosomal Lefty differ in the differentiated progenies.
27554431	7	70	theme	protein	1119:1125	arg1	expression					1127:1136	protein expression	1119:1136	protein expression	1119:1136	Differentiation of hESCs to mesenchymal cells (MSCs) or neuronal progenitor cells (NPCs) entails distinct changes in the Lefty A/Lefty B gene(s), and protein expression.
27554431	6	71	theme	N-glycan	913:920	arg1	structures					922:931	N-glycan structures	913:931	N-glycan structures of high mannose and complex nature	913:966	The exosomal- and cell-associated Lefty diverge in their proteolytic processing, and possess N-glycan structures of high mannose and complex nature.
27554431	12	72	theme	potential	1779:1787	arg1	relevance					1789:1797	the potential relevance	1775:1797	the potential relevance of Lefty-packaged exosomes for combating debilitating diseases such as cancer	1775:1875	Our studies underscore the potential relevance of Lefty-packaged exosomes for combating debilitating diseases such as cancer.
27554431	1	73	theme	transforming	156:167	arg1	TGF-β					189:193	TGF-β	189:193	TGF-β	189:193	Lefty is a member of transforming growth factor-beta (TGF-β) superfamily and a potent antagonist of the TGF-β/Nodal/Activin signaling pathway.
27554431	1	73	theme	transforming	156:167	arg1	factor-beta					176:186	transforming growth factor-beta	156:186	transforming growth factor-beta (TGF-β) superfamily	156:206	Lefty is a member of transforming growth factor-beta (TGF-β) superfamily and a potent antagonist of the TGF-β/Nodal/Activin signaling pathway.
27554431	6	74	theme	complex	953:959	arg1	nature					961:966	complex nature	953:966	complex nature	953:966	The exosomal- and cell-associated Lefty diverge in their proteolytic processing, and possess N-glycan structures of high mannose and complex nature.
27554431	0	75	from	Glycoproteins	6:18	arg1	Cells					44:48	Human Embryonic Stem Cells	23:48	Human Embryonic Stem Cells	23:48	Lefty Glycoproteins in Human Embryonic Stem Cells: Extracellular Delivery Route and Posttranslational Modification in Differentiation.
27554431	6	76	theme	mannose	941:947	arg1	structures					922:931	N-glycan structures	913:931	N-glycan structures of high mannose and complex nature	913:966	The exosomal- and cell-associated Lefty diverge in their proteolytic processing, and possess N-glycan structures of high mannose and complex nature.
27554431	8	77	theme	differentiated	1259:1272	arg1	progenies					1274:1282	the differentiated progenies	1255:1282	the differentiated progenies	1255:1282	Specifically, the proteolytic cleavage and N-glycan composition of the cell-associated and exosomal Lefty differ in the differentiated progenies.
27554431	5	78	theme	human	715:719	arg1	hESCs					727:731	hESCs	727:731	hESCs	727:731	By employing an in vitro system of human ESCs (hESCs), we observed that Lefty protein(s) are encased in exosomes for extracellular release.
27554431	5	78	theme	human	715:719	arg1	ESCs					721:724	human ESCs	715:724	human ESCs (hESCs)	715:732	By employing an in vitro system of human ESCs (hESCs), we observed that Lefty protein(s) are encased in exosomes for extracellular release.
27554431	12	79	theme	debilitating	1840:1851	arg1	diseases					1853:1860	debilitating diseases	1840:1860	debilitating diseases such as cancer	1840:1875	Our studies underscore the potential relevance of Lefty-packaged exosomes for combating debilitating diseases such as cancer.
27554431	12	79	theme	debilitating	1840:1851	arg1	cancer					1870:1875	cancer	1870:1875	cancer	1870:1875	Our studies underscore the potential relevance of Lefty-packaged exosomes for combating debilitating diseases such as cancer.
27554431	5	80	theme	protein	758:764	arg1	s					766:766	Lefty protein(s)	752:767	Lefty protein(s)	752:767	By employing an in vitro system of human ESCs (hESCs), we observed that Lefty protein(s) are encased in exosomes for extracellular release.
27554431	8	81	dep	cleavage	1169:1176	arg1	the					1153:1155	the	1153:1155	the	1153:1155	Specifically, the proteolytic cleavage and N-glycan composition of the cell-associated and exosomal Lefty differ in the differentiated progenies.
27554431	10	82	theme	Lefty	1572:1576	arg1	functions					1559:1567	the endogenous functions	1544:1567	the endogenous functions of Lefty	1544:1576	The microheterogeneity in the processing and glycosylation of Lefty protein(s) between hESCs, MSCs, and NPCs could present efficient means of diversifying the endogenous functions of Lefty.
27554431	0	83	theme	Human	23:27	arg1	Cells					44:48	Human Embryonic Stem Cells	23:48	Human Embryonic Stem Cells	23:48	Lefty Glycoproteins in Human Embryonic Stem Cells: Extracellular Delivery Route and Posttranslational Modification in Differentiation.
27554431	12	84	theme	Lefty-packaged	1802:1815	arg1	exosomes					1817:1824	Lefty-packaged exosomes	1802:1824	Lefty-packaged exosomes	1802:1824	Our studies underscore the potential relevance of Lefty-packaged exosomes for combating debilitating diseases such as cancer.
27554431	10	85	theme	Lefty	1451:1455	arg1	s					1465:1465	Lefty protein(s)	1451:1466	Lefty protein(s) between hESCs, MSCs, and NPCs	1451:1496	The microheterogeneity in the processing and glycosylation of Lefty protein(s) between hESCs, MSCs, and NPCs could present efficient means of diversifying the endogenous functions of Lefty.
27554431	0	86	theme	Stem	39:42	arg1	Cells					44:48	Human Embryonic Stem Cells	23:48	Human Embryonic Stem Cells	23:48	Lefty Glycoproteins in Human Embryonic Stem Cells: Extracellular Delivery Route and Posttranslational Modification in Differentiation.
27554431	9	87	from	signaling	1349:1357	arg1	cells					1382:1386	aggressive melanoma cells	1362:1386	aggressive melanoma cells	1362:1386	These modifications affected Lefty's inhibitory effect on Nodal signaling in aggressive melanoma cells.
27554431	11	88	theme	embryonic	1616:1624	arg1	patterning					1626:1635	embryonic patterning	1616:1635	embryonic patterning	1616:1635	Whether Lefty's diverse functions in embryonic patterning, as well as its diffusion range in the extracellular environment, are similarly affected remains to be determined.
27554431	4	89	theme	secretion	598:606	arg1	significance					616:627	the significance	612:627	the significance of their "glycan" moiety	612:652	Lefty proteins (human Lefty A and B) are secreted glycoproteins, but their mode of secretion and the significance of their "glycan" moiety remain mostly unexplored.
27554431	4	89	theme	secretion	598:606	arg1	mode					590:593	their mode	584:593	their mode of secretion	584:606	Lefty proteins (human Lefty A and B) are secreted glycoproteins, but their mode of secretion and the significance of their "glycan" moiety remain mostly unexplored.
27554431	3	90	theme	cellular	498:505	arg1	events					507:512	myriad cellular events	491:512	myriad cellular events	491:512	However, emerging studies depict Lefty as a multifaceted protein involved in myriad cellular events.
27554431	0	91	dep	Glycoproteins	6:18	arg1	Route					74:78	Extracellular Delivery Route	51:78	Extracellular Delivery Route	51:78	Lefty Glycoproteins in Human Embryonic Stem Cells: Extracellular Delivery Route and Posttranslational Modification in Differentiation.
27554431	0	91	dep	Glycoproteins	6:18	arg1	Modification					102:113	Posttranslational Modification	84:113	Posttranslational Modification	84:113	Lefty Glycoproteins in Human Embryonic Stem Cells: Extracellular Delivery Route and Posttranslational Modification in Differentiation.
27554431	0	91	dep	Glycoproteins	6:18	arg1	Glycoproteins					6:18	Lefty Glycoproteins	0:18	Lefty Glycoproteins in Human Embryonic Stem Cells: Extracellular Delivery Route and Posttranslational Modification in Differentiation.	0:133	Lefty Glycoproteins in Human Embryonic Stem Cells: Extracellular Delivery Route and Posttranslational Modification in Differentiation.
27554431	1	92	theme	TGF-β/Nodal/Activin	239:257	arg1	pathway					269:275	the TGF-β/Nodal/Activin signaling pathway	235:275	the TGF-β/Nodal/Activin signaling pathway	235:275	Lefty is a member of transforming growth factor-beta (TGF-β) superfamily and a potent antagonist of the TGF-β/Nodal/Activin signaling pathway.
27554431	2	93	theme	stem	395:398	arg1	ESCs					407:410	ESCs	407:410	ESCs	407:410	Lefty is critical in sustaining self-renewal/pluripotency status, and implicated in the differentiation of embryonic stem cells (ESCs).
27554431	2	93	theme	stem	395:398	arg1	cells					400:404	embryonic stem cells	385:404	embryonic stem cells (ESCs)	385:411	Lefty is critical in sustaining self-renewal/pluripotency status, and implicated in the differentiation of embryonic stem cells (ESCs).
27554431	9	94	theme	Nodal	1343:1347	arg1	signaling					1349:1357	Nodal signaling	1343:1357	Nodal signaling in aggressive melanoma cells	1343:1386	These modifications affected Lefty's inhibitory effect on Nodal signaling in aggressive melanoma cells.
25044149	0	0	theme	RAW	78:80	arg1	macrophages					88:98	RAW 264.7 macrophages	78:98	RAW 264.7 macrophages of polysaccharides from Talinum triangulare	78:142	Antitumor efficacy in H22 tumor bearing mice and immunoregulatory activity on RAW 264.7 macrophages of polysaccharides from Talinum triangulare.
25044149	0	1	from	efficacy	10:17	arg1	macrophages					88:98	RAW 264.7 macrophages	78:98	RAW 264.7 macrophages of polysaccharides from Talinum triangulare	78:142	Antitumor efficacy in H22 tumor bearing mice and immunoregulatory activity on RAW 264.7 macrophages of polysaccharides from Talinum triangulare.
25044149	0	1	from	efficacy	10:17	arg1	mice					40:43	H22 tumor bearing mice	22:43	H22 tumor bearing mice	22:43	Antitumor efficacy in H22 tumor bearing mice and immunoregulatory activity on RAW 264.7 macrophages of polysaccharides from Talinum triangulare.
25044149	0	1	from	efficacy	10:17	arg1	activity					66:73	immunoregulatory activity	49:73	immunoregulatory activity on RAW 264.7 macrophages of polysaccharides from Talinum triangulare	49:142	Antitumor efficacy in H22 tumor bearing mice and immunoregulatory activity on RAW 264.7 macrophages of polysaccharides from Talinum triangulare.
25044149	6	2	theme	RAW	878:880	arg1	supernatant					888:898	RAW 264.7 supernatant	878:898	RAW 264.7 supernatant	878:898	Besides, RAW 264.7 cells were activated by TTP to produce NO and the toxicity of RAW 264.7 supernatant was markedly enhanced in vitro.
25044149	3	3	theme	composition	342:352	arg1	analysis					354:361	The monosaccharide composition analysis	323:361	The monosaccharide composition analysis of TTP-IV	323:371	The monosaccharide composition analysis of TTP-IV revealed that it was a heteropolysaccharide consisting of rhamnose, arabinose, mannose and galactose with a molar ratio of 1.22 : 1.00 : 1.05 : 1.51.
25044149	6	4	theme	RAW	806:808	arg1	cells					816:820	RAW 264.7 cells	806:820	RAW 264.7 cells	806:820	Besides, RAW 264.7 cells were activated by TTP to produce NO and the toxicity of RAW 264.7 supernatant was markedly enhanced in vitro.
25044149	7	5	theme	IL-1β	967:971	arg1	levels					936:941	The levels	932:941	The levels of iNOS, TLR2, TLR4 and IL-1β	932:971	The levels of iNOS, TLR2, TLR4 and IL-1β were obviously increased by TTP.
25044149	4	6	theme	Kunming	660:666	arg1	mice					668:671	H22-bearing Kunming mice	648:671	H22-bearing Kunming mice	648:671	The results of the in vivo study showed that TTP (200 mg per kg bw) significantly inhibited the growth of tumor by 49.07% in H22-bearing Kunming mice.
25044149	3	7	dep	ratio	487:491	arg1	 1.05 					509:514	 1.05 	509:514	 1.05 	509:514	The monosaccharide composition analysis of TTP-IV revealed that it was a heteropolysaccharide consisting of rhamnose, arabinose, mannose and galactose with a molar ratio of 1.22 : 1.00 : 1.05 : 1.51.
25044149	3	7	dep	ratio	487:491	arg1	 1.00 					502:507	 1.00 	502:507	 1.00 	502:507	The monosaccharide composition analysis of TTP-IV revealed that it was a heteropolysaccharide consisting of rhamnose, arabinose, mannose and galactose with a molar ratio of 1.22 : 1.00 : 1.05 : 1.51.
25044149	3	7	dep	ratio	487:491	arg1	 1.51					516:520	 1.51	516:520	 1.51	516:520	The monosaccharide composition analysis of TTP-IV revealed that it was a heteropolysaccharide consisting of rhamnose, arabinose, mannose and galactose with a molar ratio of 1.22 : 1.00 : 1.05 : 1.51.
25044149	4	8	theme	in	542:543	arg1	study					550:554	the in vivo study	538:554	the in vivo study	538:554	The results of the in vivo study showed that TTP (200 mg per kg bw) significantly inhibited the growth of tumor by 49.07% in H22-bearing Kunming mice.
25044149	0	9	theme	polysaccharides	103:117	arg1	macrophages					88:98	RAW 264.7 macrophages	78:98	RAW 264.7 macrophages of polysaccharides from Talinum triangulare	78:142	Antitumor efficacy in H22 tumor bearing mice and immunoregulatory activity on RAW 264.7 macrophages of polysaccharides from Talinum triangulare.
25044149	0	10	from	mice	40:43	arg1	macrophages					88:98	RAW 264.7 macrophages	78:98	RAW 264.7 macrophages of polysaccharides from Talinum triangulare	78:142	Antitumor efficacy in H22 tumor bearing mice and immunoregulatory activity on RAW 264.7 macrophages of polysaccharides from Talinum triangulare.
25044149	3	11	with	galactose	464:472	arg1	ratio					487:491	a molar ratio	479:491	a molar ratio of 1.22 : 1.00 : 1.05 : 1.51	479:520	The monosaccharide composition analysis of TTP-IV revealed that it was a heteropolysaccharide consisting of rhamnose, arabinose, mannose and galactose with a molar ratio of 1.22 : 1.00 : 1.05 : 1.51.
25044149	4	12	theme	H22-bearing	648:658	arg1	mice					668:671	H22-bearing Kunming mice	648:671	H22-bearing Kunming mice	648:671	The results of the in vivo study showed that TTP (200 mg per kg bw) significantly inhibited the growth of tumor by 49.07% in H22-bearing Kunming mice.
25044149	1	13	theme	paper	161:165	arg1	aim					149:151	The aim	145:151	The aim of this paper	145:165	The aim of this paper was to study the antitumor and immunoregulatory activities of a polysaccharide (TTP) from Talinum triangulare.
25044149	3	14	theme	monosaccharide	327:340	arg1	composition					342:352	The monosaccharide composition	323:352	The monosaccharide composition analysis of TTP-IV	323:371	The monosaccharide composition analysis of TTP-IV revealed that it was a heteropolysaccharide consisting of rhamnose, arabinose, mannose and galactose with a molar ratio of 1.22 : 1.00 : 1.05 : 1.51.
25044149	4	15	theme	study	550:554	arg1	results					527:533	The results	523:533	The results of the in vivo study	523:554	The results of the in vivo study showed that TTP (200 mg per kg bw) significantly inhibited the growth of tumor by 49.07% in H22-bearing Kunming mice.
25044149	8	16	theme	antitumor	1070:1078	arg1	material					1100:1107	a potent antitumor and immunoenhancing material	1061:1107	a potent antitumor and immunoenhancing material in functional food	1061:1126	Therefore, it is suggested that TTP can be utilized as a potent antitumor and immunoenhancing material in functional food.
25044149	8	16	theme	antitumor	1070:1078	arg1	TTP					1038:1040	TTP	1038:1040	TTP	1038:1040	Therefore, it is suggested that TTP can be utilized as a potent antitumor and immunoenhancing material in functional food.
25044149	5	17	theme	murine	706:711	arg1	macrophages					713:723	primary murine macrophages	698:723	primary murine macrophages	698:723	In vitro, the growth of primary murine macrophages was promoted by TTP in a dose- and time-dependent manner significantly.
25044149	7	18	theme	TLR2	952:955	arg1	levels					936:941	The levels	932:941	The levels of iNOS, TLR2, TLR4 and IL-1β	932:971	The levels of iNOS, TLR2, TLR4 and IL-1β were obviously increased by TTP.
25044149	2	19	theme	TTP-IV	302:307	arg1	kDa					318:320	49.9 kDa	313:320	49.9 kDa	313:320	The molecular weight of TTP-IV was 49.9 kDa.
25044149	2	19	theme	TTP-IV	302:307	arg1	weight					292:297	The molecular weight	278:297	The molecular weight of TTP-IV	278:307	The molecular weight of TTP-IV was 49.9 kDa.
25044149	5	20	theme	time-dependent	760:773	arg1	manner					775:780	a dose- and time-dependent manner	748:780	a dose- and time-dependent manner	748:780	In vitro, the growth of primary murine macrophages was promoted by TTP in a dose- and time-dependent manner significantly.
25044149	0	21	theme	Antitumor	0:8	arg1	efficacy					10:17	Antitumor efficacy	0:17	Antitumor efficacy in H22 tumor bearing mice and immunoregulatory activity on RAW 264.7 macrophages of polysaccharides from Talinum triangulare.	0:143	Antitumor efficacy in H22 tumor bearing mice and immunoregulatory activity on RAW 264.7 macrophages of polysaccharides from Talinum triangulare.
25044149	1	22	theme	Talinum	257:263	arg1	triangulare					265:275	Talinum triangulare	257:275	Talinum triangulare	257:275	The aim of this paper was to study the antitumor and immunoregulatory activities of a polysaccharide (TTP) from Talinum triangulare.
25044149	1	23	from	triangulare	265:275	arg1	TTP					247:249	TTP	247:249	TTP	247:249	The aim of this paper was to study the antitumor and immunoregulatory activities of a polysaccharide (TTP) from Talinum triangulare.
25044149	1	23	from	triangulare	265:275	arg1	activities					215:224	the antitumor and immunoregulatory activities	180:224	the antitumor and immunoregulatory activities of a polysaccharide (TTP) from Talinum triangulare	180:275	The aim of this paper was to study the antitumor and immunoregulatory activities of a polysaccharide (TTP) from Talinum triangulare.
25044149	1	23	from	triangulare	265:275	arg1	polysaccharide					231:244	a polysaccharide	229:244	a polysaccharide (TTP) from Talinum triangulare	229:275	The aim of this paper was to study the antitumor and immunoregulatory activities of a polysaccharide (TTP) from Talinum triangulare.
25044149	5	24	theme	dose-	750:754	arg1	manner					775:780	a dose- and time-dependent manner	748:780	a dose- and time-dependent manner	748:780	In vitro, the growth of primary murine macrophages was promoted by TTP in a dose- and time-dependent manner significantly.
25044149	7	25	theme	TLR4	958:961	arg1	levels					936:941	The levels	932:941	The levels of iNOS, TLR2, TLR4 and IL-1β	932:971	The levels of iNOS, TLR2, TLR4 and IL-1β were obviously increased by TTP.
25044149	8	26	theme	immunoenhancing	1084:1098	arg1	material					1100:1107	a potent antitumor and immunoenhancing material	1061:1107	a potent antitumor and immunoenhancing material in functional food	1061:1126	Therefore, it is suggested that TTP can be utilized as a potent antitumor and immunoenhancing material in functional food.
25044149	8	26	theme	immunoenhancing	1084:1098	arg1	TTP					1038:1040	TTP	1038:1040	TTP	1038:1040	Therefore, it is suggested that TTP can be utilized as a potent antitumor and immunoenhancing material in functional food.
25044149	0	27	theme	H22	22:24	arg1	mice					40:43	H22 tumor bearing mice	22:43	H22 tumor bearing mice	22:43	Antitumor efficacy in H22 tumor bearing mice and immunoregulatory activity on RAW 264.7 macrophages of polysaccharides from Talinum triangulare.
25044149	5	28	theme	macrophages	713:723	arg1	growth					688:693	the growth	684:693	the growth of primary murine macrophages	684:723	In vitro, the growth of primary murine macrophages was promoted by TTP in a dose- and time-dependent manner significantly.
25044149	0	29	theme	Talinum	124:130	arg1	triangulare					132:142	Talinum triangulare	124:142	Talinum triangulare	124:142	Antitumor efficacy in H22 tumor bearing mice and immunoregulatory activity on RAW 264.7 macrophages of polysaccharides from Talinum triangulare.
25044149	8	30	theme	functional	1112:1121	arg1	food					1123:1126	functional food	1112:1126	functional food	1112:1126	Therefore, it is suggested that TTP can be utilized as a potent antitumor and immunoenhancing material in functional food.
25044149	3	31	theme	TTP-IV	366:371	arg1	analysis					354:361	The monosaccharide composition analysis	323:361	The monosaccharide composition analysis of TTP-IV	323:371	The monosaccharide composition analysis of TTP-IV revealed that it was a heteropolysaccharide consisting of rhamnose, arabinose, mannose and galactose with a molar ratio of 1.22 : 1.00 : 1.05 : 1.51.
25044149	2	32	theme	molecular	282:290	arg1	kDa					318:320	49.9 kDa	313:320	49.9 kDa	313:320	The molecular weight of TTP-IV was 49.9 kDa.
25044149	2	32	theme	molecular	282:290	arg1	weight					292:297	The molecular weight	278:297	The molecular weight of TTP-IV	278:307	The molecular weight of TTP-IV was 49.9 kDa.
25044149	1	33	theme	antitumor	184:192	arg1	activities					215:224	the antitumor and immunoregulatory activities	180:224	the antitumor and immunoregulatory activities of a polysaccharide (TTP) from Talinum triangulare	180:275	The aim of this paper was to study the antitumor and immunoregulatory activities of a polysaccharide (TTP) from Talinum triangulare.
25044149	0	34	theme	bearing	32:38	arg1	mice					40:43	H22 tumor bearing mice	22:43	H22 tumor bearing mice	22:43	Antitumor efficacy in H22 tumor bearing mice and immunoregulatory activity on RAW 264.7 macrophages of polysaccharides from Talinum triangulare.
25044149	4	35	theme	tumor	629:633	arg1	growth					619:624	the growth	615:624	the growth of tumor	615:633	The results of the in vivo study showed that TTP (200 mg per kg bw) significantly inhibited the growth of tumor by 49.07% in H22-bearing Kunming mice.
25044149	3	36	with	mannose	452:458	arg1	ratio					487:491	a molar ratio	479:491	a molar ratio of 1.22 : 1.00 : 1.05 : 1.51	479:520	The monosaccharide composition analysis of TTP-IV revealed that it was a heteropolysaccharide consisting of rhamnose, arabinose, mannose and galactose with a molar ratio of 1.22 : 1.00 : 1.05 : 1.51.
25044149	4	37	dep	in	542:543	arg1	vivo					545:548	vivo	545:548	vivo	545:548	The results of the in vivo study showed that TTP (200 mg per kg bw) significantly inhibited the growth of tumor by 49.07% in H22-bearing Kunming mice.
25044149	0	38	theme	tumor	26:30	arg1	mice					40:43	H22 tumor bearing mice	22:43	H22 tumor bearing mice	22:43	Antitumor efficacy in H22 tumor bearing mice and immunoregulatory activity on RAW 264.7 macrophages of polysaccharides from Talinum triangulare.
25044149	0	39	from	activity	66:73	arg1	macrophages					88:98	RAW 264.7 macrophages	78:98	RAW 264.7 macrophages of polysaccharides from Talinum triangulare	78:142	Antitumor efficacy in H22 tumor bearing mice and immunoregulatory activity on RAW 264.7 macrophages of polysaccharides from Talinum triangulare.
25044149	8	40	from	material	1100:1107	arg1	food					1123:1126	functional food	1112:1126	functional food	1112:1126	Therefore, it is suggested that TTP can be utilized as a potent antitumor and immunoenhancing material in functional food.
25044149	1	41	theme	immunoregulatory	198:213	arg1	activities					215:224	the antitumor and immunoregulatory activities	180:224	the antitumor and immunoregulatory activities of a polysaccharide (TTP) from Talinum triangulare	180:275	The aim of this paper was to study the antitumor and immunoregulatory activities of a polysaccharide (TTP) from Talinum triangulare.
25044149	8	42	theme	potent	1063:1068	arg1	material					1100:1107	a potent antitumor and immunoenhancing material	1061:1107	a potent antitumor and immunoenhancing material in functional food	1061:1126	Therefore, it is suggested that TTP can be utilized as a potent antitumor and immunoenhancing material in functional food.
25044149	8	42	theme	potent	1063:1068	arg1	TTP					1038:1040	TTP	1038:1040	TTP	1038:1040	Therefore, it is suggested that TTP can be utilized as a potent antitumor and immunoenhancing material in functional food.
25044149	8	43	used	utilized	1049:1056	arg2	material					1100:1107	a potent antitumor and immunoenhancing material	1061:1107	a potent antitumor and immunoenhancing material in functional food	1061:1126	Therefore, it is suggested that TTP can be utilized as a potent antitumor and immunoenhancing material in functional food.
25044149	8	43	used	utilized	1049:1056	arg2	TTP					1038:1040	TTP	1038:1040	TTP	1038:1040	Therefore, it is suggested that TTP can be utilized as a potent antitumor and immunoenhancing material in functional food.
25044149	5	44	theme	primary	698:704	arg1	macrophages					713:723	primary murine macrophages	698:723	primary murine macrophages	698:723	In vitro, the growth of primary murine macrophages was promoted by TTP in a dose- and time-dependent manner significantly.
25044149	7	45	theme	iNOS	946:949	arg1	levels					936:941	The levels	932:941	The levels of iNOS, TLR2, TLR4 and IL-1β	932:971	The levels of iNOS, TLR2, TLR4 and IL-1β were obviously increased by TTP.
25044149	4	46	dep	TTP	568:570	arg1	mg					577:578	200 mg	573:578	200 mg per kg bw	573:588	The results of the in vivo study showed that TTP (200 mg per kg bw) significantly inhibited the growth of tumor by 49.07% in H22-bearing Kunming mice.
25044149	3	47	theme	molar	481:485	arg1	ratio					487:491	a molar ratio	479:491	a molar ratio of 1.22 : 1.00 : 1.05 : 1.51	479:520	The monosaccharide composition analysis of TTP-IV revealed that it was a heteropolysaccharide consisting of rhamnose, arabinose, mannose and galactose with a molar ratio of 1.22 : 1.00 : 1.05 : 1.51.
25044149	4	48	from	%	643:643	arg1	mice					668:671	H22-bearing Kunming mice	648:671	H22-bearing Kunming mice	648:671	The results of the in vivo study showed that TTP (200 mg per kg bw) significantly inhibited the growth of tumor by 49.07% in H22-bearing Kunming mice.
25044149	1	49	from	activities	215:224	arg1	triangulare					265:275	Talinum triangulare	257:275	Talinum triangulare	257:275	The aim of this paper was to study the antitumor and immunoregulatory activities of a polysaccharide (TTP) from Talinum triangulare.
25044149	0	50	from	macrophages	88:98	arg1	triangulare					132:142	Talinum triangulare	124:142	Talinum triangulare	124:142	Antitumor efficacy in H22 tumor bearing mice and immunoregulatory activity on RAW 264.7 macrophages of polysaccharides from Talinum triangulare.
25044149	0	50	from	macrophages	88:98	arg1	efficacy					10:17	Antitumor efficacy	0:17	Antitumor efficacy in H22 tumor bearing mice and immunoregulatory activity on RAW 264.7 macrophages of polysaccharides from Talinum triangulare.	0:143	Antitumor efficacy in H22 tumor bearing mice and immunoregulatory activity on RAW 264.7 macrophages of polysaccharides from Talinum triangulare.
25044149	0	51	from	triangulare	132:142	arg1	macrophages					88:98	RAW 264.7 macrophages	78:98	RAW 264.7 macrophages of polysaccharides from Talinum triangulare	78:142	Antitumor efficacy in H22 tumor bearing mice and immunoregulatory activity on RAW 264.7 macrophages of polysaccharides from Talinum triangulare.
25044149	0	51	from	triangulare	132:142	arg1	polysaccharides					103:117	polysaccharides	103:117	polysaccharides from Talinum triangulare	103:142	Antitumor efficacy in H22 tumor bearing mice and immunoregulatory activity on RAW 264.7 macrophages of polysaccharides from Talinum triangulare.
25044149	3	52	theme	1.22 	496:500	arg1	ratio					487:491	a molar ratio	479:491	a molar ratio of 1.22 : 1.00 : 1.05 : 1.51	479:520	The monosaccharide composition analysis of TTP-IV revealed that it was a heteropolysaccharide consisting of rhamnose, arabinose, mannose and galactose with a molar ratio of 1.22 : 1.00 : 1.05 : 1.51.
25044149	0	53	theme	immunoregulatory	49:64	arg1	activity					66:73	immunoregulatory activity	49:73	immunoregulatory activity on RAW 264.7 macrophages of polysaccharides from Talinum triangulare	49:142	Antitumor efficacy in H22 tumor bearing mice and immunoregulatory activity on RAW 264.7 macrophages of polysaccharides from Talinum triangulare.
25044149	6	54	theme	supernatant	888:898	arg1	toxicity					866:873	the toxicity	862:873	the toxicity of RAW 264.7 supernatant	862:898	Besides, RAW 264.7 cells were activated by TTP to produce NO and the toxicity of RAW 264.7 supernatant was markedly enhanced in vitro.
25044149	1	55	theme	polysaccharide	231:244	arg1	activities					215:224	the antitumor and immunoregulatory activities	180:224	the antitumor and immunoregulatory activities of a polysaccharide (TTP) from Talinum triangulare	180:275	The aim of this paper was to study the antitumor and immunoregulatory activities of a polysaccharide (TTP) from Talinum triangulare.
27118045	0	0	theme	chemical	82:89	arg1	sensor					91:96	chemical sensor	82:96	chemical sensor	82:96	Rapid, facile microwave-assisted synthesis of xanthan gum grafted polyaniline for chemical sensor.
27118045	7	1	theme	composite	1102:1110	arg1	film					1112:1115	mwXG-g-PANi composite film	1090:1115	mwXG-g-PANi composite film	1090:1115	Electrical conductivity of XG and mwXG-g-PANi composite film was performed.
27118045	1	2	from	terms	181:185	arg1	productive					167:176	productive	167:176	productive	167:176	Grafting method, through microwave radiation procedure is extremely productive in terms of time consumption, cost effectiveness and environmental friendliness.
27118045	2	3	theme	aniline	361:367	arg1	grafting					349:356	grafting	349:356	grafting of aniline (ANi)	349:373	In this study, conductive and thermally stable composite (mwXG-g-PANi) was synthesized by grafting of aniline (ANi) on to xanthan gum (XG) using catalytic weight of initiator, ammonium peroxydisulfate in the process of microwave irradiation in an aqueous medium.
27118045	9	4	theme	rapid	1296:1300	arg1	sensing					1302:1308	a rapid sensing	1294:1308	a rapid sensing	1294:1308	The mwXG-g-PANi, effectively integrated and handled, are NH3 sensitive and exhibit a rapid sensing in presence of NH3 vapor.
27118045	7	5	theme	film	1112:1115	arg1	conductivity					1067:1078	Electrical conductivity	1056:1078	Electrical conductivity of XG and mwXG-g-PANi composite film	1056:1115	Electrical conductivity of XG and mwXG-g-PANi composite film was performed.
27118045	10	6	theme	room	1377:1380	arg1	temperature					1382:1392	superior room temperature	1368:1392	superior room temperature sensing performance	1368:1412	Chemiresistive NH3 sensors with superior room temperature sensing performance were produced with sensor response of 905 at 1ppb and 90% recovery within few second.
27118045	2	7	theme	microwave	478:486	arg1	irradiation					488:498	microwave irradiation	478:498	microwave irradiation	478:498	In this study, conductive and thermally stable composite (mwXG-g-PANi) was synthesized by grafting of aniline (ANi) on to xanthan gum (XG) using catalytic weight of initiator, ammonium peroxydisulfate in the process of microwave irradiation in an aqueous medium.
27118045	2	8	dep	peroxydisulfate	444:458	arg1	ammonium					435:442	initiator, ammonium peroxydisulfate	424:458	ammonium	435:442	In this study, conductive and thermally stable composite (mwXG-g-PANi) was synthesized by grafting of aniline (ANi) on to xanthan gum (XG) using catalytic weight of initiator, ammonium peroxydisulfate in the process of microwave irradiation in an aqueous medium.
27118045	1	9	from	productive	167:176	arg1	terms					181:185	terms	181:185	terms of time consumption, cost effectiveness and environmental friendliness	181:256	Grafting method, through microwave radiation procedure is extremely productive in terms of time consumption, cost effectiveness and environmental friendliness.
27118045	2	10	theme	stable	299:304	arg1	mwXG-g-PANi					317:327	mwXG-g-PANi	317:327	mwXG-g-PANi	317:327	In this study, conductive and thermally stable composite (mwXG-g-PANi) was synthesized by grafting of aniline (ANi) on to xanthan gum (XG) using catalytic weight of initiator, ammonium peroxydisulfate in the process of microwave irradiation in an aqueous medium.
27118045	2	10	theme	stable	299:304	arg1	composite					306:314	conductive and thermally stable composite	274:314	conductive and thermally stable composite (mwXG-g-PANi)	274:328	In this study, conductive and thermally stable composite (mwXG-g-PANi) was synthesized by grafting of aniline (ANi) on to xanthan gum (XG) using catalytic weight of initiator, ammonium peroxydisulfate in the process of microwave irradiation in an aqueous medium.
27118045	4	11	theme	monomer	684:690	arg1	microwave					656:664	microwave	656:664	microwave	656:664	The influence of altering the microwave power, exposure time of microwave, concentration of monomer and the amount of initiator of graft polymerization were studied over the grafting parameters, for example, grafting percentage (%G) and grafting efficiency (%E).
27118045	4	11	theme	monomer	684:690	arg1	amount					700:705	the amount	696:705	the amount of initiator of graft polymerization	696:742	The influence of altering the microwave power, exposure time of microwave, concentration of monomer and the amount of initiator of graft polymerization were studied over the grafting parameters, for example, grafting percentage (%G) and grafting efficiency (%E).
27118045	4	11	theme	monomer	684:690	arg1	initiator					710:718	initiator	710:718	initiator of graft polymerization	710:742	The influence of altering the microwave power, exposure time of microwave, concentration of monomer and the amount of initiator of graft polymerization were studied over the grafting parameters, for example, grafting percentage (%G) and grafting efficiency (%E).
27118045	4	11	theme	monomer	684:690	arg1	concentration					667:679	concentration	667:679	concentration of monomer	667:690	The influence of altering the microwave power, exposure time of microwave, concentration of monomer and the amount of initiator of graft polymerization were studied over the grafting parameters, for example, grafting percentage (%G) and grafting efficiency (%E).
27118045	4	11	theme	monomer	684:690	arg1	monomer					684:690	monomer	684:690	monomer	684:690	The influence of altering the microwave power, exposure time of microwave, concentration of monomer and the amount of initiator of graft polymerization were studied over the grafting parameters, for example, grafting percentage (%G) and grafting efficiency (%E).
27118045	4	11	theme	monomer	684:690	arg1	time					648:651	the microwave power, exposure time	618:651	time	648:651	The influence of altering the microwave power, exposure time of microwave, concentration of monomer and the amount of initiator of graft polymerization were studied over the grafting parameters, for example, grafting percentage (%G) and grafting efficiency (%E).
27118045	6	12	theme	conventional	1035:1046	arg1	method					1048:1053	the conventional method	1031:1053	the conventional method	1031:1053	The outcome demonstrates that the microwave irradiation strategy can increase the reaction rate by 72 times over the conventional method.
27118045	5	13	theme	%	867:867	arg1	G					868:868	The maximum %G and %E	855:875	G	868:868	The maximum %G and %E achieved was 172 and 74.13 respectively.
27118045	5	13	theme	%	867:867	arg1	172					890:892	172	890:892	172	890:892	The maximum %G and %E achieved was 172 and 74.13 respectively.
27118045	4	14	theme	microwave	656:664	arg1	microwave					656:664	microwave	656:664	microwave	656:664	The influence of altering the microwave power, exposure time of microwave, concentration of monomer and the amount of initiator of graft polymerization were studied over the grafting parameters, for example, grafting percentage (%G) and grafting efficiency (%E).
27118045	4	14	theme	microwave	656:664	arg1	amount					700:705	the amount	696:705	the amount of initiator of graft polymerization	696:742	The influence of altering the microwave power, exposure time of microwave, concentration of monomer and the amount of initiator of graft polymerization were studied over the grafting parameters, for example, grafting percentage (%G) and grafting efficiency (%E).
27118045	4	14	theme	microwave	656:664	arg1	initiator					710:718	initiator	710:718	initiator of graft polymerization	710:742	The influence of altering the microwave power, exposure time of microwave, concentration of monomer and the amount of initiator of graft polymerization were studied over the grafting parameters, for example, grafting percentage (%G) and grafting efficiency (%E).
27118045	4	14	theme	microwave	656:664	arg1	concentration					667:679	concentration	667:679	concentration of monomer	667:690	The influence of altering the microwave power, exposure time of microwave, concentration of monomer and the amount of initiator of graft polymerization were studied over the grafting parameters, for example, grafting percentage (%G) and grafting efficiency (%E).
27118045	4	14	theme	microwave	656:664	arg1	monomer					684:690	monomer	684:690	monomer	684:690	The influence of altering the microwave power, exposure time of microwave, concentration of monomer and the amount of initiator of graft polymerization were studied over the grafting parameters, for example, grafting percentage (%G) and grafting efficiency (%E).
27118045	4	14	theme	microwave	656:664	arg1	time					648:651	the microwave power, exposure time	618:651	time	648:651	The influence of altering the microwave power, exposure time of microwave, concentration of monomer and the amount of initiator of graft polymerization were studied over the grafting parameters, for example, grafting percentage (%G) and grafting efficiency (%E).
27118045	2	15	theme	irradiation	488:498	arg1	process					467:473	the process	463:473	the process of microwave irradiation in an aqueous medium	463:519	In this study, conductive and thermally stable composite (mwXG-g-PANi) was synthesized by grafting of aniline (ANi) on to xanthan gum (XG) using catalytic weight of initiator, ammonium peroxydisulfate in the process of microwave irradiation in an aqueous medium.
27118045	10	16	dep	recovery	1472:1479	arg1	%					1470:1470	%	1470:1470	%	1470:1470	Chemiresistive NH3 sensors with superior room temperature sensing performance were produced with sensor response of 905 at 1ppb and 90% recovery within few second.
27118045	4	17	theme	initiator	710:718	arg1	microwave					656:664	microwave	656:664	microwave	656:664	The influence of altering the microwave power, exposure time of microwave, concentration of monomer and the amount of initiator of graft polymerization were studied over the grafting parameters, for example, grafting percentage (%G) and grafting efficiency (%E).
27118045	4	17	theme	initiator	710:718	arg1	amount					700:705	the amount	696:705	the amount of initiator of graft polymerization	696:742	The influence of altering the microwave power, exposure time of microwave, concentration of monomer and the amount of initiator of graft polymerization were studied over the grafting parameters, for example, grafting percentage (%G) and grafting efficiency (%E).
27118045	4	17	theme	initiator	710:718	arg1	initiator					710:718	initiator	710:718	initiator of graft polymerization	710:742	The influence of altering the microwave power, exposure time of microwave, concentration of monomer and the amount of initiator of graft polymerization were studied over the grafting parameters, for example, grafting percentage (%G) and grafting efficiency (%E).
27118045	4	17	theme	initiator	710:718	arg1	concentration					667:679	concentration	667:679	concentration of monomer	667:690	The influence of altering the microwave power, exposure time of microwave, concentration of monomer and the amount of initiator of graft polymerization were studied over the grafting parameters, for example, grafting percentage (%G) and grafting efficiency (%E).
27118045	4	17	theme	initiator	710:718	arg1	monomer					684:690	monomer	684:690	monomer	684:690	The influence of altering the microwave power, exposure time of microwave, concentration of monomer and the amount of initiator of graft polymerization were studied over the grafting parameters, for example, grafting percentage (%G) and grafting efficiency (%E).
27118045	4	17	theme	initiator	710:718	arg1	time					648:651	the microwave power, exposure time	618:651	time	648:651	The influence of altering the microwave power, exposure time of microwave, concentration of monomer and the amount of initiator of graft polymerization were studied over the grafting parameters, for example, grafting percentage (%G) and grafting efficiency (%E).
27118045	9	18	theme	vapor	1329:1333	arg1	presence					1313:1320	presence	1313:1320	presence of NH3 vapor	1313:1333	The mwXG-g-PANi, effectively integrated and handled, are NH3 sensitive and exhibit a rapid sensing in presence of NH3 vapor.
27118045	2	19	theme	xanthan	381:387	arg1	XG					394:395	XG	394:395	XG	394:395	In this study, conductive and thermally stable composite (mwXG-g-PANi) was synthesized by grafting of aniline (ANi) on to xanthan gum (XG) using catalytic weight of initiator, ammonium peroxydisulfate in the process of microwave irradiation in an aqueous medium.
27118045	2	19	theme	xanthan	381:387	arg1	gum					389:391	xanthan gum	381:391	xanthan gum (XG) using catalytic weight of initiator, ammonium peroxydisulfate in the process of microwave irradiation in an aqueous medium	381:519	In this study, conductive and thermally stable composite (mwXG-g-PANi) was synthesized by grafting of aniline (ANi) on to xanthan gum (XG) using catalytic weight of initiator, ammonium peroxydisulfate in the process of microwave irradiation in an aqueous medium.
27118045	0	20	theme	xanthan	46:52	arg1	gum					54:56	xanthan gum	46:56	xanthan gum	46:56	Rapid, facile microwave-assisted synthesis of xanthan gum grafted polyaniline for chemical sensor.
27118045	10	21	dep	%	1470:1470	arg1	1ppb					1459:1462	1ppb	1459:1462	1ppb	1459:1462	Chemiresistive NH3 sensors with superior room temperature sensing performance were produced with sensor response of 905 at 1ppb and 90% recovery within few second.
27118045	10	21	dep	%	1470:1470	arg1	90					1468:1469	90	1468:1469	90	1468:1469	Chemiresistive NH3 sensors with superior room temperature sensing performance were produced with sensor response of 905 at 1ppb and 90% recovery within few second.
27118045	10	22	theme	superior	1368:1375	arg1	temperature					1382:1392	superior room temperature	1368:1392	superior room temperature sensing performance	1368:1412	Chemiresistive NH3 sensors with superior room temperature sensing performance were produced with sensor response of 905 at 1ppb and 90% recovery within few second.
27118045	2	23	theme	conductive	274:283	arg1	mwXG-g-PANi					317:327	mwXG-g-PANi	317:327	mwXG-g-PANi	317:327	In this study, conductive and thermally stable composite (mwXG-g-PANi) was synthesized by grafting of aniline (ANi) on to xanthan gum (XG) using catalytic weight of initiator, ammonium peroxydisulfate in the process of microwave irradiation in an aqueous medium.
27118045	2	23	theme	conductive	274:283	arg1	composite					306:314	conductive and thermally stable composite	274:314	conductive and thermally stable composite (mwXG-g-PANi)	274:328	In this study, conductive and thermally stable composite (mwXG-g-PANi) was synthesized by grafting of aniline (ANi) on to xanthan gum (XG) using catalytic weight of initiator, ammonium peroxydisulfate in the process of microwave irradiation in an aqueous medium.
27118045	6	24	theme	microwave	952:960	arg1	irradiation					962:972	the microwave irradiation	948:972	the microwave irradiation strategy	948:981	The outcome demonstrates that the microwave irradiation strategy can increase the reaction rate by 72 times over the conventional method.
27118045	7	25	theme	Electrical	1056:1065	arg1	conductivity					1067:1078	Electrical conductivity	1056:1078	Electrical conductivity of XG and mwXG-g-PANi composite film	1056:1115	Electrical conductivity of XG and mwXG-g-PANi composite film was performed.
27118045	10	26	theme	sensor	1433:1438	arg1	response					1440:1447	sensor response	1433:1447	sensor response of 905 at 1ppb and 90% recovery within few second	1433:1497	Chemiresistive NH3 sensors with superior room temperature sensing performance were produced with sensor response of 905 at 1ppb and 90% recovery within few second.
27118045	10	27	theme	NH3	1351:1353	arg1	sensors					1355:1361	Chemiresistive NH3 sensors	1336:1361	Chemiresistive NH3 sensors with superior room temperature sensing performance	1336:1412	Chemiresistive NH3 sensors with superior room temperature sensing performance were produced with sensor response of 905 at 1ppb and 90% recovery within few second.
27118045	3	28	theme	mwXG-g-PANi	539:549	arg1	synthesis					526:534	The synthesis	522:534	The synthesis of mwXG-g-PANi	522:549	The synthesis of mwXG-g-PANi were confirm by FTIR, XRD, TGA, and SEM.
27118045	10	29	theme	recovery	1472:1479	arg1	response					1440:1447	sensor response	1433:1447	sensor response of 905 at 1ppb and 90% recovery within few second	1433:1497	Chemiresistive NH3 sensors with superior room temperature sensing performance were produced with sensor response of 905 at 1ppb and 90% recovery within few second.
27118045	8	30	theme	grafted	1147:1153	arg1	film					1162:1165	The fabricated grafted sample film	1132:1165	The fabricated grafted sample film	1132:1165	The fabricated grafted sample film were then examined for the chemical sensor.
27118045	0	31	theme	Rapid	0:4	arg1	synthesis					33:41	Rapid, facile microwave-assisted synthesis	0:41	Rapid, facile microwave-assisted synthesis of xanthan gum	0:56	Rapid, facile microwave-assisted synthesis of xanthan gum grafted polyaniline for chemical sensor.
27118045	10	32	theme	Chemiresistive	1336:1349	arg1	sensors					1355:1361	Chemiresistive NH3 sensors	1336:1361	Chemiresistive NH3 sensors with superior room temperature sensing performance	1336:1412	Chemiresistive NH3 sensors with superior room temperature sensing performance were produced with sensor response of 905 at 1ppb and 90% recovery within few second.
27118045	4	33	dep	time	648:651	arg1	exposure					639:646	the microwave power, exposure time	618:651	exposure	639:646	The influence of altering the microwave power, exposure time of microwave, concentration of monomer and the amount of initiator of graft polymerization were studied over the grafting parameters, for example, grafting percentage (%G) and grafting efficiency (%E).
27118045	1	34	theme	time	190:193	arg1	consumption					195:205	time consumption	190:205	time consumption	190:205	Grafting method, through microwave radiation procedure is extremely productive in terms of time consumption, cost effectiveness and environmental friendliness.
27118045	0	35	theme	microwave-assisted	14:31	arg1	synthesis					33:41	Rapid, facile microwave-assisted synthesis	0:41	Rapid, facile microwave-assisted synthesis of xanthan gum	0:56	Rapid, facile microwave-assisted synthesis of xanthan gum grafted polyaniline for chemical sensor.
27118045	8	36	theme	chemical	1194:1201	arg1	sensor					1203:1208	the chemical sensor	1190:1208	the chemical sensor	1190:1208	The fabricated grafted sample film were then examined for the chemical sensor.
27118045	1	37	theme	consumption	195:205	arg1	terms					181:185	terms	181:185	terms of time consumption, cost effectiveness and environmental friendliness	181:256	Grafting method, through microwave radiation procedure is extremely productive in terms of time consumption, cost effectiveness and environmental friendliness.
27118045	6	38	theme	reaction	1000:1007	arg1	rate					1009:1012	the reaction rate	996:1012	the reaction rate	996:1012	The outcome demonstrates that the microwave irradiation strategy can increase the reaction rate by 72 times over the conventional method.
27118045	4	39	theme	grafting	829:836	arg1	efficiency					838:847	grafting efficiency	829:847	grafting efficiency (%E)	829:852	The influence of altering the microwave power, exposure time of microwave, concentration of monomer and the amount of initiator of graft polymerization were studied over the grafting parameters, for example, grafting percentage (%G) and grafting efficiency (%E).
27118045	4	39	theme	grafting	829:836	arg1	E					851:851	%E	850:851	%E	850:851	The influence of altering the microwave power, exposure time of microwave, concentration of monomer and the amount of initiator of graft polymerization were studied over the grafting parameters, for example, grafting percentage (%G) and grafting efficiency (%E).
27118045	0	40	dep	grafted	58:64	arg1	polyaniline					66:76	polyaniline	66:76	grafted polyaniline for chemical sensor	58:96	Rapid, facile microwave-assisted synthesis of xanthan gum grafted polyaniline for chemical sensor.
27118045	8	41	theme	sample	1155:1160	arg1	film					1162:1165	The fabricated grafted sample film	1132:1165	The fabricated grafted sample film	1132:1165	The fabricated grafted sample film were then examined for the chemical sensor.
27118045	2	42	theme	peroxydisulfate	444:458	arg1	weight					414:419	catalytic weight	404:419	catalytic weight of initiator, ammonium peroxydisulfate	404:458	In this study, conductive and thermally stable composite (mwXG-g-PANi) was synthesized by grafting of aniline (ANi) on to xanthan gum (XG) using catalytic weight of initiator, ammonium peroxydisulfate in the process of microwave irradiation in an aqueous medium.
27118045	4	43	theme	%	850:850	arg1	efficiency					838:847	grafting efficiency	829:847	grafting efficiency (%E)	829:852	The influence of altering the microwave power, exposure time of microwave, concentration of monomer and the amount of initiator of graft polymerization were studied over the grafting parameters, for example, grafting percentage (%G) and grafting efficiency (%E).
27118045	4	43	theme	%	850:850	arg1	E					851:851	%E	850:851	%E	850:851	The influence of altering the microwave power, exposure time of microwave, concentration of monomer and the amount of initiator of graft polymerization were studied over the grafting parameters, for example, grafting percentage (%G) and grafting efficiency (%E).
27118045	7	44	theme	XG	1083:1084	arg1	conductivity					1067:1078	Electrical conductivity	1056:1078	Electrical conductivity of XG and mwXG-g-PANi composite film	1056:1115	Electrical conductivity of XG and mwXG-g-PANi composite film was performed.
27118045	8	45	theme	fabricated	1136:1145	arg1	film					1162:1165	The fabricated grafted sample film	1132:1165	The fabricated grafted sample film	1132:1165	The fabricated grafted sample film were then examined for the chemical sensor.
27118045	1	46	theme	cost	208:211	arg1	effectiveness					213:225	cost effectiveness	208:225	cost effectiveness	208:225	Grafting method, through microwave radiation procedure is extremely productive in terms of time consumption, cost effectiveness and environmental friendliness.
27118045	6	47	theme	irradiation	962:972	arg1	strategy					974:981	the microwave irradiation strategy	948:981	the microwave irradiation strategy	948:981	The outcome demonstrates that the microwave irradiation strategy can increase the reaction rate by 72 times over the conventional method.
27118045	2	48	theme	aqueous	506:512	arg1	medium					514:519	an aqueous medium	503:519	an aqueous medium	503:519	In this study, conductive and thermally stable composite (mwXG-g-PANi) was synthesized by grafting of aniline (ANi) on to xanthan gum (XG) using catalytic weight of initiator, ammonium peroxydisulfate in the process of microwave irradiation in an aqueous medium.
27118045	2	49	theme	catalytic	404:412	arg1	weight					414:419	catalytic weight	404:419	catalytic weight of initiator, ammonium peroxydisulfate	404:458	In this study, conductive and thermally stable composite (mwXG-g-PANi) was synthesized by grafting of aniline (ANi) on to xanthan gum (XG) using catalytic weight of initiator, ammonium peroxydisulfate in the process of microwave irradiation in an aqueous medium.
27118045	1	50	theme	effectiveness	213:225	arg1	terms					181:185	terms	181:185	terms of time consumption, cost effectiveness and environmental friendliness	181:256	Grafting method, through microwave radiation procedure is extremely productive in terms of time consumption, cost effectiveness and environmental friendliness.
27118045	0	51	theme	gum	54:56	arg1	synthesis					33:41	Rapid, facile microwave-assisted synthesis	0:41	Rapid, facile microwave-assisted synthesis of xanthan gum	0:56	Rapid, facile microwave-assisted synthesis of xanthan gum grafted polyaniline for chemical sensor.
27118045	9	52	dep	mwXG-g-PANi	1215:1225	arg1	integrated					1240:1249	integrated	1240:1249	integrated	1240:1249	The mwXG-g-PANi, effectively integrated and handled, are NH3 sensitive and exhibit a rapid sensing in presence of NH3 vapor.
27118045	9	52	dep	mwXG-g-PANi	1215:1225	arg1	handled					1255:1261	handled	1255:1261	handled	1255:1261	The mwXG-g-PANi, effectively integrated and handled, are NH3 sensitive and exhibit a rapid sensing in presence of NH3 vapor.
27118045	2	53	from	process	467:473	arg1	medium					514:519	an aqueous medium	503:519	an aqueous medium	503:519	In this study, conductive and thermally stable composite (mwXG-g-PANi) was synthesized by grafting of aniline (ANi) on to xanthan gum (XG) using catalytic weight of initiator, ammonium peroxydisulfate in the process of microwave irradiation in an aqueous medium.
27118045	4	54	theme	grafting	766:773	arg1	parameters					775:784	the grafting parameters	762:784	the grafting parameters	762:784	The influence of altering the microwave power, exposure time of microwave, concentration of monomer and the amount of initiator of graft polymerization were studied over the grafting parameters, for example, grafting percentage (%G) and grafting efficiency (%E).
27118045	2	55	theme	initiator	424:432	arg1	peroxydisulfate					444:458	initiator, ammonium peroxydisulfate	424:458	peroxydisulfate	444:458	In this study, conductive and thermally stable composite (mwXG-g-PANi) was synthesized by grafting of aniline (ANi) on to xanthan gum (XG) using catalytic weight of initiator, ammonium peroxydisulfate in the process of microwave irradiation in an aqueous medium.
27118045	4	56	theme	%	821:821	arg1	percentage					809:818	percentage	809:818	percentage (%G)	809:823	The influence of altering the microwave power, exposure time of microwave, concentration of monomer and the amount of initiator of graft polymerization were studied over the grafting parameters, for example, grafting percentage (%G) and grafting efficiency (%E).
27118045	4	56	theme	%	821:821	arg1	G					822:822	%G	821:822	%G	821:822	The influence of altering the microwave power, exposure time of microwave, concentration of monomer and the amount of initiator of graft polymerization were studied over the grafting parameters, for example, grafting percentage (%G) and grafting efficiency (%E).
27118045	4	57	theme	polymerization	729:742	arg1	initiator					710:718	initiator	710:718	initiator of graft polymerization	710:742	The influence of altering the microwave power, exposure time of microwave, concentration of monomer and the amount of initiator of graft polymerization were studied over the grafting parameters, for example, grafting percentage (%G) and grafting efficiency (%E).
27118045	5	58	theme	%	874:874	arg1	E					875:875	The maximum %G and %E	855:875	E	875:875	The maximum %G and %E achieved was 172 and 74.13 respectively.
27118045	4	59	theme	graft	723:727	arg1	polymerization					729:742	graft polymerization	723:742	graft polymerization	723:742	The influence of altering the microwave power, exposure time of microwave, concentration of monomer and the amount of initiator of graft polymerization were studied over the grafting parameters, for example, grafting percentage (%G) and grafting efficiency (%E).
27118045	4	60	theme	microwave	622:630	arg1	time					648:651	the microwave power, exposure time	618:651	time	648:651	The influence of altering the microwave power, exposure time of microwave, concentration of monomer and the amount of initiator of graft polymerization were studied over the grafting parameters, for example, grafting percentage (%G) and grafting efficiency (%E).
27118045	1	61	theme	microwave	124:132	arg1	procedure					144:152	microwave radiation procedure	124:152	microwave radiation procedure	124:152	Grafting method, through microwave radiation procedure is extremely productive in terms of time consumption, cost effectiveness and environmental friendliness.
27118045	5	62	theme	maximum	859:865	arg1	G					868:868	The maximum %G and %E	855:875	G	868:868	The maximum %G and %E achieved was 172 and 74.13 respectively.
27118045	5	62	theme	maximum	859:865	arg1	172					890:892	172	890:892	172	890:892	The maximum %G and %E achieved was 172 and 74.13 respectively.
27118045	10	63	with	sensors	1355:1361	arg1	temperature					1382:1392	superior room temperature	1368:1392	superior room temperature sensing performance	1368:1412	Chemiresistive NH3 sensors with superior room temperature sensing performance were produced with sensor response of 905 at 1ppb and 90% recovery within few second.
27118045	1	64	theme	radiation	134:142	arg1	procedure					144:152	microwave radiation procedure	124:152	microwave radiation procedure	124:152	Grafting method, through microwave radiation procedure is extremely productive in terms of time consumption, cost effectiveness and environmental friendliness.
27118045	1	65	theme	environmental	231:243	arg1	friendliness					245:256	environmental friendliness	231:256	environmental friendliness	231:256	Grafting method, through microwave radiation procedure is extremely productive in terms of time consumption, cost effectiveness and environmental friendliness.
27118045	4	66	theme	power	632:636	arg1	time					648:651	the microwave power, exposure time	618:651	time	648:651	The influence of altering the microwave power, exposure time of microwave, concentration of monomer and the amount of initiator of graft polymerization were studied over the grafting parameters, for example, grafting percentage (%G) and grafting efficiency (%E).
27118045	9	67	theme	NH3	1325:1327	arg1	vapor					1329:1333	NH3 vapor	1325:1333	NH3 vapor	1325:1333	The mwXG-g-PANi, effectively integrated and handled, are NH3 sensitive and exhibit a rapid sensing in presence of NH3 vapor.
27118045	1	68	theme	friendliness	245:256	arg1	terms					181:185	terms	181:185	terms of time consumption, cost effectiveness and environmental friendliness	181:256	Grafting method, through microwave radiation procedure is extremely productive in terms of time consumption, cost effectiveness and environmental friendliness.
27118045	0	69	dep	Rapid	0:4	arg1	facile					7:12	facile	7:12	facile	7:12	Rapid, facile microwave-assisted synthesis of xanthan gum grafted polyaniline for chemical sensor.
27118045	1	70	dep	method	108:113	arg1	productive					167:176	productive	167:176	productive	167:176	Grafting method, through microwave radiation procedure is extremely productive in terms of time consumption, cost effectiveness and environmental friendliness.
27118045	7	71	theme	mwXG-g-PANi	1090:1100	arg1	film					1112:1115	mwXG-g-PANi composite film	1090:1115	mwXG-g-PANi composite film	1090:1115	Electrical conductivity of XG and mwXG-g-PANi composite film was performed.
24274482	3	0	contain	containing	506:515	arg1	composites					495:504	the CIP-agar composites	482:504	the CIP-agar composites containing different concentrations (5, 10, 15, 20, 25 and 30% w/w) of magnetically aligned and non-aligned CIP in the agar matrix	482:635	Heat transfer behavior of the CIP-agar composites containing different concentrations (5, 10, 15, 20, 25 and 30% w/w) of magnetically aligned and non-aligned CIP in the agar matrix was studied using photothermal radiometry (PTR) in the back-propagation emission configuration.
24274482	3	0	contain	containing	506:515	arg2	%					567:567	5, 10, 15, 20, 25 and 30% w/w	543:571	5, 10, 15, 20, 25 and 30% w/w	543:571	Heat transfer behavior of the CIP-agar composites containing different concentrations (5, 10, 15, 20, 25 and 30% w/w) of magnetically aligned and non-aligned CIP in the agar matrix was studied using photothermal radiometry (PTR) in the back-propagation emission configuration.
24274482	3	0	contain	containing	506:515	arg2	concentrations					527:540	different concentrations	517:540	different concentrations (5, 10, 15, 20, 25 and 30% w/w) of magnetically aligned and non-aligned CIP in the agar matrix	517:635	Heat transfer behavior of the CIP-agar composites containing different concentrations (5, 10, 15, 20, 25 and 30% w/w) of magnetically aligned and non-aligned CIP in the agar matrix was studied using photothermal radiometry (PTR) in the back-propagation emission configuration.
24274482	6	1	theme	perfect	1107:1113	arg1	matrix					1115:1120	a perfect matrix	1105:1120	a perfect matrix	1105:1120	Agar served as a perfect matrix to be used with CIP, and CIP-agar composites magnetically aligned at 20% CIP concentration can be considered as promising 'smart' material for hyperthermia treatments in the biomedical field.
24274482	6	1	theme	perfect	1107:1113	arg1	Agar					1090:1093	Agar	1090:1093	Agar	1090:1093	Agar served as a perfect matrix to be used with CIP, and CIP-agar composites magnetically aligned at 20% CIP concentration can be considered as promising 'smart' material for hyperthermia treatments in the biomedical field.
24274482	3	2	theme	photothermal	655:666	arg1	PTR					680:682	PTR	680:682	PTR	680:682	Heat transfer behavior of the CIP-agar composites containing different concentrations (5, 10, 15, 20, 25 and 30% w/w) of magnetically aligned and non-aligned CIP in the agar matrix was studied using photothermal radiometry (PTR) in the back-propagation emission configuration.
24274482	3	2	theme	photothermal	655:666	arg1	radiometry					668:677	photothermal radiometry	655:677	photothermal radiometry (PTR) in the back-propagation emission configuration	655:730	Heat transfer behavior of the CIP-agar composites containing different concentrations (5, 10, 15, 20, 25 and 30% w/w) of magnetically aligned and non-aligned CIP in the agar matrix was studied using photothermal radiometry (PTR) in the back-propagation emission configuration.
24274482	3	3	theme	different	517:525	arg1	%					567:567	5, 10, 15, 20, 25 and 30% w/w	543:571	5, 10, 15, 20, 25 and 30% w/w	543:571	Heat transfer behavior of the CIP-agar composites containing different concentrations (5, 10, 15, 20, 25 and 30% w/w) of magnetically aligned and non-aligned CIP in the agar matrix was studied using photothermal radiometry (PTR) in the back-propagation emission configuration.
24274482	3	3	theme	different	517:525	arg1	concentrations					527:540	different concentrations	517:540	different concentrations (5, 10, 15, 20, 25 and 30% w/w) of magnetically aligned and non-aligned CIP in the agar matrix	517:635	Heat transfer behavior of the CIP-agar composites containing different concentrations (5, 10, 15, 20, 25 and 30% w/w) of magnetically aligned and non-aligned CIP in the agar matrix was studied using photothermal radiometry (PTR) in the back-propagation emission configuration.
24274482	6	4	theme	biomedical	1296:1305	arg1	field					1307:1311	the biomedical field	1292:1311	the biomedical field	1292:1311	Agar served as a perfect matrix to be used with CIP, and CIP-agar composites magnetically aligned at 20% CIP concentration can be considered as promising 'smart' material for hyperthermia treatments in the biomedical field.
24274482	4	5	theme	non-aligned	792:802	arg1	CIP					804:806	aligned and non-aligned CIP	780:806	aligned and non-aligned CIP under magnetic field	780:827	The morphology of the CIP-agar composites with aligned and non-aligned CIP under magnetic field was also evaluated by scanning electron microscopy (SEM).
24274482	1	6	theme	particles	103:111	arg1	Composites					80:89	Composites	80:89	Composites of magnetic particles into polymeric matrices	80:135	Composites of magnetic particles into polymeric matrices have received increasing research interest due to their capacity to respond to external magnetic or electromagnetic fields.
24274482	1	6	theme	particles	103:111	arg1	particles					103:111	magnetic particles	94:111	magnetic particles	94:111	Composites of magnetic particles into polymeric matrices have received increasing research interest due to their capacity to respond to external magnetic or electromagnetic fields.
24274482	3	7	from	concentrations	527:540	arg1	matrix					630:635	the agar matrix	621:635	the agar matrix	621:635	Heat transfer behavior of the CIP-agar composites containing different concentrations (5, 10, 15, 20, 25 and 30% w/w) of magnetically aligned and non-aligned CIP in the agar matrix was studied using photothermal radiometry (PTR) in the back-propagation emission configuration.
24274482	0	8	theme	Thermal	0:6	arg1	characterization					8:23	Thermal characterization	0:23	Thermal characterization of magnetically aligned carbonyl iron/agar composites.	0:78	Thermal characterization of magnetically aligned carbonyl iron/agar composites.
24274482	6	9	theme	hyperthermia	1265:1276	arg1	treatments					1278:1287	hyperthermia treatments	1265:1287	hyperthermia treatments in the biomedical field	1265:1311	Agar served as a perfect matrix to be used with CIP, and CIP-agar composites magnetically aligned at 20% CIP concentration can be considered as promising 'smart' material for hyperthermia treatments in the biomedical field.
24274482	5	10	theme	thermal	1044:1050	arg1	diffusivity					1052:1062	the thermal diffusivity	1040:1062	the thermal diffusivity	1040:1062	The results revealed a dominant effect of CIP concentration over the alignment patterns induced by the magnetic field, which agrees with the behavior of the thermal diffusivity and thermal conductivity.
24274482	6	11	theme	CIP	1195:1197	arg1	concentration					1199:1211	20% CIP concentration	1191:1211	20% CIP concentration	1191:1211	Agar served as a perfect matrix to be used with CIP, and CIP-agar composites magnetically aligned at 20% CIP concentration can be considered as promising 'smart' material for hyperthermia treatments in the biomedical field.
24274482	5	12	theme	CIP	929:931	arg1	concentration					933:945	CIP concentration	929:945	CIP concentration	929:945	The results revealed a dominant effect of CIP concentration over the alignment patterns induced by the magnetic field, which agrees with the behavior of the thermal diffusivity and thermal conductivity.
24274482	3	13	from	CIP	614:616	arg1	matrix					630:635	the agar matrix	621:635	the agar matrix	621:635	Heat transfer behavior of the CIP-agar composites containing different concentrations (5, 10, 15, 20, 25 and 30% w/w) of magnetically aligned and non-aligned CIP in the agar matrix was studied using photothermal radiometry (PTR) in the back-propagation emission configuration.
24274482	5	14	theme	diffusivity	1052:1062	arg1	behavior					1028:1035	the behavior	1024:1035	the behavior of the thermal diffusivity and thermal conductivity	1024:1087	The results revealed a dominant effect of CIP concentration over the alignment patterns induced by the magnetic field, which agrees with the behavior of the thermal diffusivity and thermal conductivity.
24274482	6	15	theme	%	1193:1193	arg1	concentration					1199:1211	20% CIP concentration	1191:1211	20% CIP concentration	1191:1211	Agar served as a perfect matrix to be used with CIP, and CIP-agar composites magnetically aligned at 20% CIP concentration can be considered as promising 'smart' material for hyperthermia treatments in the biomedical field.
24274482	1	16	theme	magnetic	94:101	arg1	particles					103:111	magnetic particles	94:111	magnetic particles	94:111	Composites of magnetic particles into polymeric matrices have received increasing research interest due to their capacity to respond to external magnetic or electromagnetic fields.
24274482	2	17	theme	particles	403:411	arg1	matrix					366:371	build the matrix	356:371	build the matrix of the magnetic carbonyl iron particles (CIP)	356:417	In this study, agar from Gelidium robustum has been chosen as natural biocompatible polymer to build the matrix of the magnetic carbonyl iron particles (CIP) for their uses in biomedical fields.
24274482	3	18	from	radiometry	668:677	arg1	configuration					718:730	the back-propagation emission configuration	688:730	the back-propagation emission configuration	688:730	Heat transfer behavior of the CIP-agar composites containing different concentrations (5, 10, 15, 20, 25 and 30% w/w) of magnetically aligned and non-aligned CIP in the agar matrix was studied using photothermal radiometry (PTR) in the back-propagation emission configuration.
24274482	3	19	from	matrix	630:635	arg1	%					567:567	5, 10, 15, 20, 25 and 30% w/w	543:571	5, 10, 15, 20, 25 and 30% w/w	543:571	Heat transfer behavior of the CIP-agar composites containing different concentrations (5, 10, 15, 20, 25 and 30% w/w) of magnetically aligned and non-aligned CIP in the agar matrix was studied using photothermal radiometry (PTR) in the back-propagation emission configuration.
24274482	3	19	from	matrix	630:635	arg1	concentrations					527:540	different concentrations	517:540	different concentrations (5, 10, 15, 20, 25 and 30% w/w) of magnetically aligned and non-aligned CIP in the agar matrix	517:635	Heat transfer behavior of the CIP-agar composites containing different concentrations (5, 10, 15, 20, 25 and 30% w/w) of magnetically aligned and non-aligned CIP in the agar matrix was studied using photothermal radiometry (PTR) in the back-propagation emission configuration.
24274482	6	20	theme	20	1191:1192	arg1	%					1193:1193	%	1193:1193	%	1193:1193	Agar served as a perfect matrix to be used with CIP, and CIP-agar composites magnetically aligned at 20% CIP concentration can be considered as promising 'smart' material for hyperthermia treatments in the biomedical field.
24274482	4	21	with	morphology	737:746	arg1	CIP					804:806	aligned and non-aligned CIP	780:806	aligned and non-aligned CIP under magnetic field	780:827	The morphology of the CIP-agar composites with aligned and non-aligned CIP under magnetic field was also evaluated by scanning electron microscopy (SEM).
24274482	2	22	theme	biocompatible	331:343	arg1	polymer					345:351	natural biocompatible polymer	323:351	natural biocompatible polymer to build the matrix of the magnetic carbonyl iron particles (CIP)	323:417	In this study, agar from Gelidium robustum has been chosen as natural biocompatible polymer to build the matrix of the magnetic carbonyl iron particles (CIP) for their uses in biomedical fields.
24274482	2	22	theme	biocompatible	331:343	arg1	agar					276:279	agar	276:279	agar from Gelidium robustum	276:302	In this study, agar from Gelidium robustum has been chosen as natural biocompatible polymer to build the matrix of the magnetic carbonyl iron particles (CIP) for their uses in biomedical fields.
24274482	6	23	from	treatments	1278:1287	arg1	field					1307:1311	the biomedical field	1292:1311	the biomedical field	1292:1311	Agar served as a perfect matrix to be used with CIP, and CIP-agar composites magnetically aligned at 20% CIP concentration can be considered as promising 'smart' material for hyperthermia treatments in the biomedical field.
24274482	2	24	theme	iron	398:401	arg1	particles					403:411	the magnetic carbonyl iron particles	376:411	the magnetic carbonyl iron particles (CIP)	376:417	In this study, agar from Gelidium robustum has been chosen as natural biocompatible polymer to build the matrix of the magnetic carbonyl iron particles (CIP) for their uses in biomedical fields.
24274482	2	24	theme	iron	398:401	arg1	CIP					414:416	CIP	414:416	CIP	414:416	In this study, agar from Gelidium robustum has been chosen as natural biocompatible polymer to build the matrix of the magnetic carbonyl iron particles (CIP) for their uses in biomedical fields.
24274482	2	25	theme	biomedical	437:446	arg1	fields					448:453	biomedical fields	437:453	biomedical fields	437:453	In this study, agar from Gelidium robustum has been chosen as natural biocompatible polymer to build the matrix of the magnetic carbonyl iron particles (CIP) for their uses in biomedical fields.
24274482	3	26	theme	back-propagation	692:707	arg1	configuration					718:730	the back-propagation emission configuration	688:730	the back-propagation emission configuration	688:730	Heat transfer behavior of the CIP-agar composites containing different concentrations (5, 10, 15, 20, 25 and 30% w/w) of magnetically aligned and non-aligned CIP in the agar matrix was studied using photothermal radiometry (PTR) in the back-propagation emission configuration.
24274482	4	27	theme	magnetic	814:821	arg1	field					823:827	magnetic field	814:827	magnetic field	814:827	The morphology of the CIP-agar composites with aligned and non-aligned CIP under magnetic field was also evaluated by scanning electron microscopy (SEM).
24274482	2	28	theme	natural	323:329	arg1	polymer					345:351	natural biocompatible polymer	323:351	natural biocompatible polymer to build the matrix of the magnetic carbonyl iron particles (CIP)	323:417	In this study, agar from Gelidium robustum has been chosen as natural biocompatible polymer to build the matrix of the magnetic carbonyl iron particles (CIP) for their uses in biomedical fields.
24274482	2	28	theme	natural	323:329	arg1	agar					276:279	agar	276:279	agar from Gelidium robustum	276:302	In this study, agar from Gelidium robustum has been chosen as natural biocompatible polymer to build the matrix of the magnetic carbonyl iron particles (CIP) for their uses in biomedical fields.
24274482	2	29	theme	carbonyl	389:396	arg1	particles					403:411	the magnetic carbonyl iron particles	376:411	the magnetic carbonyl iron particles (CIP)	376:417	In this study, agar from Gelidium robustum has been chosen as natural biocompatible polymer to build the matrix of the magnetic carbonyl iron particles (CIP) for their uses in biomedical fields.
24274482	2	29	theme	carbonyl	389:396	arg1	CIP					414:416	CIP	414:416	CIP	414:416	In this study, agar from Gelidium robustum has been chosen as natural biocompatible polymer to build the matrix of the magnetic carbonyl iron particles (CIP) for their uses in biomedical fields.
24274482	3	30	theme	emission	709:716	arg1	configuration					718:730	the back-propagation emission configuration	688:730	the back-propagation emission configuration	688:730	Heat transfer behavior of the CIP-agar composites containing different concentrations (5, 10, 15, 20, 25 and 30% w/w) of magnetically aligned and non-aligned CIP in the agar matrix was studied using photothermal radiometry (PTR) in the back-propagation emission configuration.
24274482	5	31	theme	alignment	956:964	arg1	patterns					966:973	the alignment patterns	952:973	the alignment patterns induced by the magnetic field, which agrees with the behavior of the thermal diffusivity and thermal conductivity	952:1087	The results revealed a dominant effect of CIP concentration over the alignment patterns induced by the magnetic field, which agrees with the behavior of the thermal diffusivity and thermal conductivity.
24274482	6	32	theme	CIP-agar	1147:1154	arg1	composites					1156:1165	CIP-agar composites	1147:1165	CIP-agar composites magnetically aligned at 20% CIP concentration	1147:1211	Agar served as a perfect matrix to be used with CIP, and CIP-agar composites magnetically aligned at 20% CIP concentration can be considered as promising 'smart' material for hyperthermia treatments in the biomedical field.
24274482	6	32	theme	CIP-agar	1147:1154	arg1	material					1252:1259	promising 'smart' material	1234:1259	promising 'smart' material for hyperthermia treatments in the biomedical field	1234:1311	Agar served as a perfect matrix to be used with CIP, and CIP-agar composites magnetically aligned at 20% CIP concentration can be considered as promising 'smart' material for hyperthermia treatments in the biomedical field.
24274482	1	33	theme	polymeric	118:126	arg1	matrices					128:135	polymeric matrices	118:135	polymeric matrices	118:135	Composites of magnetic particles into polymeric matrices have received increasing research interest due to their capacity to respond to external magnetic or electromagnetic fields.
24274482	3	34	theme	transfer	461:468	arg1	behavior					470:477	Heat transfer behavior	456:477	Heat transfer behavior of the CIP-agar composites containing different concentrations (5, 10, 15, 20, 25 and 30% w/w) of magnetically aligned and non-aligned CIP in the agar matrix	456:635	Heat transfer behavior of the CIP-agar composites containing different concentrations (5, 10, 15, 20, 25 and 30% w/w) of magnetically aligned and non-aligned CIP in the agar matrix was studied using photothermal radiometry (PTR) in the back-propagation emission configuration.
24274482	5	35	theme	conductivity	1076:1087	arg1	behavior					1028:1035	the behavior	1024:1035	the behavior of the thermal diffusivity and thermal conductivity	1024:1087	The results revealed a dominant effect of CIP concentration over the alignment patterns induced by the magnetic field, which agrees with the behavior of the thermal diffusivity and thermal conductivity.
24274482	3	36	theme	non-aligned	602:612	arg1	CIP					614:616	magnetically aligned and non-aligned CIP	577:616	magnetically aligned and non-aligned CIP in the agar matrix	577:635	Heat transfer behavior of the CIP-agar composites containing different concentrations (5, 10, 15, 20, 25 and 30% w/w) of magnetically aligned and non-aligned CIP in the agar matrix was studied using photothermal radiometry (PTR) in the back-propagation emission configuration.
24274482	5	37	theme	dominant	910:917	arg1	effect					919:924	a dominant effect	908:924	a dominant effect of CIP concentration over the alignment patterns induced by the magnetic field, which agrees with the behavior of the thermal diffusivity and thermal conductivity	908:1087	The results revealed a dominant effect of CIP concentration over the alignment patterns induced by the magnetic field, which agrees with the behavior of the thermal diffusivity and thermal conductivity.
24274482	0	38	theme	carbonyl	49:56	arg1	composites					68:77	magnetically aligned carbonyl iron/agar composites	28:77	magnetically aligned carbonyl iron/agar composites	28:77	Thermal characterization of magnetically aligned carbonyl iron/agar composites.
24274482	3	39	theme	CIP	614:616	arg1	%					567:567	5, 10, 15, 20, 25 and 30% w/w	543:571	5, 10, 15, 20, 25 and 30% w/w	543:571	Heat transfer behavior of the CIP-agar composites containing different concentrations (5, 10, 15, 20, 25 and 30% w/w) of magnetically aligned and non-aligned CIP in the agar matrix was studied using photothermal radiometry (PTR) in the back-propagation emission configuration.
24274482	3	39	theme	CIP	614:616	arg1	concentrations					527:540	different concentrations	517:540	different concentrations (5, 10, 15, 20, 25 and 30% w/w) of magnetically aligned and non-aligned CIP in the agar matrix	517:635	Heat transfer behavior of the CIP-agar composites containing different concentrations (5, 10, 15, 20, 25 and 30% w/w) of magnetically aligned and non-aligned CIP in the agar matrix was studied using photothermal radiometry (PTR) in the back-propagation emission configuration.
24274482	4	40	theme	aligned	780:786	arg1	CIP					804:806	aligned and non-aligned CIP	780:806	aligned and non-aligned CIP under magnetic field	780:827	The morphology of the CIP-agar composites with aligned and non-aligned CIP under magnetic field was also evaluated by scanning electron microscopy (SEM).
24274482	6	41	used	used	1128:1131	arg2	Agar					1090:1093	Agar	1090:1093	Agar	1090:1093	Agar served as a perfect matrix to be used with CIP, and CIP-agar composites magnetically aligned at 20% CIP concentration can be considered as promising 'smart' material for hyperthermia treatments in the biomedical field.
24274482	6	41	used	used	1128:1131	arg2	matrix					1115:1120	a perfect matrix	1105:1120	a perfect matrix	1105:1120	Agar served as a perfect matrix to be used with CIP, and CIP-agar composites magnetically aligned at 20% CIP concentration can be considered as promising 'smart' material for hyperthermia treatments in the biomedical field.
24274482	0	42	theme	aligned	41:47	arg1	composites					68:77	magnetically aligned carbonyl iron/agar composites	28:77	magnetically aligned carbonyl iron/agar composites	28:77	Thermal characterization of magnetically aligned carbonyl iron/agar composites.
24274482	3	43	theme	aligned	590:596	arg1	CIP					614:616	magnetically aligned and non-aligned CIP	577:616	magnetically aligned and non-aligned CIP in the agar matrix	577:635	Heat transfer behavior of the CIP-agar composites containing different concentrations (5, 10, 15, 20, 25 and 30% w/w) of magnetically aligned and non-aligned CIP in the agar matrix was studied using photothermal radiometry (PTR) in the back-propagation emission configuration.
24274482	1	44	theme	electromagnetic	237:251	arg1	fields					253:258	external magnetic or electromagnetic fields	216:258	fields	253:258	Composites of magnetic particles into polymeric matrices have received increasing research interest due to their capacity to respond to external magnetic or electromagnetic fields.
24274482	0	45	theme	composites	68:77	arg1	characterization					8:23	Thermal characterization	0:23	Thermal characterization of magnetically aligned carbonyl iron/agar composites.	0:78	Thermal characterization of magnetically aligned carbonyl iron/agar composites.
24274482	4	46	theme	electron	860:867	arg1	microscopy					869:878	scanning electron microscopy	851:878	scanning electron microscopy (SEM)	851:884	The morphology of the CIP-agar composites with aligned and non-aligned CIP under magnetic field was also evaluated by scanning electron microscopy (SEM).
24274482	4	46	theme	electron	860:867	arg1	SEM					881:883	SEM	881:883	SEM	881:883	The morphology of the CIP-agar composites with aligned and non-aligned CIP under magnetic field was also evaluated by scanning electron microscopy (SEM).
24274482	2	47	dep	their	423:427	arg1	uses					429:432	uses	429:432	uses	429:432	In this study, agar from Gelidium robustum has been chosen as natural biocompatible polymer to build the matrix of the magnetic carbonyl iron particles (CIP) for their uses in biomedical fields.
24274482	3	48	theme	CIP-agar	486:493	arg1	composites					495:504	the CIP-agar composites	482:504	the CIP-agar composites containing different concentrations (5, 10, 15, 20, 25 and 30% w/w) of magnetically aligned and non-aligned CIP in the agar matrix	482:635	Heat transfer behavior of the CIP-agar composites containing different concentrations (5, 10, 15, 20, 25 and 30% w/w) of magnetically aligned and non-aligned CIP in the agar matrix was studied using photothermal radiometry (PTR) in the back-propagation emission configuration.
24274482	0	49	theme	iron/agar	58:66	arg1	composites					68:77	magnetically aligned carbonyl iron/agar composites	28:77	magnetically aligned carbonyl iron/agar composites	28:77	Thermal characterization of magnetically aligned carbonyl iron/agar composites.
24274482	4	50	theme	scanning	851:858	arg1	microscopy					869:878	scanning electron microscopy	851:878	scanning electron microscopy (SEM)	851:884	The morphology of the CIP-agar composites with aligned and non-aligned CIP under magnetic field was also evaluated by scanning electron microscopy (SEM).
24274482	4	50	theme	scanning	851:858	arg1	SEM					881:883	SEM	881:883	SEM	881:883	The morphology of the CIP-agar composites with aligned and non-aligned CIP under magnetic field was also evaluated by scanning electron microscopy (SEM).
24274482	3	51	theme	agar	625:628	arg1	matrix					630:635	the agar matrix	621:635	the agar matrix	621:635	Heat transfer behavior of the CIP-agar composites containing different concentrations (5, 10, 15, 20, 25 and 30% w/w) of magnetically aligned and non-aligned CIP in the agar matrix was studied using photothermal radiometry (PTR) in the back-propagation emission configuration.
24274482	5	52	theme	magnetic	990:997	arg1	field					999:1003	the magnetic field	986:1003	the magnetic field	986:1003	The results revealed a dominant effect of CIP concentration over the alignment patterns induced by the magnetic field, which agrees with the behavior of the thermal diffusivity and thermal conductivity.
24274482	3	53	theme	composites	495:504	arg1	behavior					470:477	Heat transfer behavior	456:477	Heat transfer behavior of the CIP-agar composites containing different concentrations (5, 10, 15, 20, 25 and 30% w/w) of magnetically aligned and non-aligned CIP in the agar matrix	456:635	Heat transfer behavior of the CIP-agar composites containing different concentrations (5, 10, 15, 20, 25 and 30% w/w) of magnetically aligned and non-aligned CIP in the agar matrix was studied using photothermal radiometry (PTR) in the back-propagation emission configuration.
24274482	2	54	from	robustum	295:302	arg1	polymer					345:351	natural biocompatible polymer	323:351	natural biocompatible polymer to build the matrix of the magnetic carbonyl iron particles (CIP)	323:417	In this study, agar from Gelidium robustum has been chosen as natural biocompatible polymer to build the matrix of the magnetic carbonyl iron particles (CIP) for their uses in biomedical fields.
24274482	2	54	from	robustum	295:302	arg1	agar					276:279	agar	276:279	agar from Gelidium robustum	276:302	In this study, agar from Gelidium robustum has been chosen as natural biocompatible polymer to build the matrix of the magnetic carbonyl iron particles (CIP) for their uses in biomedical fields.
24274482	6	55	theme	promising	1234:1242	arg1	material					1252:1259	promising 'smart' material	1234:1259	promising 'smart' material for hyperthermia treatments in the biomedical field	1234:1311	Agar served as a perfect matrix to be used with CIP, and CIP-agar composites magnetically aligned at 20% CIP concentration can be considered as promising 'smart' material for hyperthermia treatments in the biomedical field.
24274482	6	55	theme	promising	1234:1242	arg1	composites					1156:1165	CIP-agar composites	1147:1165	CIP-agar composites magnetically aligned at 20% CIP concentration	1147:1211	Agar served as a perfect matrix to be used with CIP, and CIP-agar composites magnetically aligned at 20% CIP concentration can be considered as promising 'smart' material for hyperthermia treatments in the biomedical field.
24274482	5	56	theme	concentration	933:945	arg1	effect					919:924	a dominant effect	908:924	a dominant effect of CIP concentration over the alignment patterns induced by the magnetic field, which agrees with the behavior of the thermal diffusivity and thermal conductivity	908:1087	The results revealed a dominant effect of CIP concentration over the alignment patterns induced by the magnetic field, which agrees with the behavior of the thermal diffusivity and thermal conductivity.
24274482	1	57	theme	research	162:169	arg1	interest					171:178	research interest	162:178	research interest	162:178	Composites of magnetic particles into polymeric matrices have received increasing research interest due to their capacity to respond to external magnetic or electromagnetic fields.
24274482	3	58	theme	Heat	456:459	arg1	behavior					470:477	Heat transfer behavior	456:477	Heat transfer behavior of the CIP-agar composites containing different concentrations (5, 10, 15, 20, 25 and 30% w/w) of magnetically aligned and non-aligned CIP in the agar matrix	456:635	Heat transfer behavior of the CIP-agar composites containing different concentrations (5, 10, 15, 20, 25 and 30% w/w) of magnetically aligned and non-aligned CIP in the agar matrix was studied using photothermal radiometry (PTR) in the back-propagation emission configuration.
24274482	4	59	theme	composites	764:773	arg1	morphology					737:746	The morphology	733:746	The morphology of the CIP-agar composites with aligned and non-aligned CIP under magnetic field	733:827	The morphology of the CIP-agar composites with aligned and non-aligned CIP under magnetic field was also evaluated by scanning electron microscopy (SEM).
24274482	2	60	theme	magnetic	380:387	arg1	particles					403:411	the magnetic carbonyl iron particles	376:411	the magnetic carbonyl iron particles (CIP)	376:417	In this study, agar from Gelidium robustum has been chosen as natural biocompatible polymer to build the matrix of the magnetic carbonyl iron particles (CIP) for their uses in biomedical fields.
24274482	2	60	theme	magnetic	380:387	arg1	CIP					414:416	CIP	414:416	CIP	414:416	In this study, agar from Gelidium robustum has been chosen as natural biocompatible polymer to build the matrix of the magnetic carbonyl iron particles (CIP) for their uses in biomedical fields.
24274482	5	61	theme	thermal	1068:1074	arg1	conductivity					1076:1087	thermal conductivity	1068:1087	thermal conductivity	1068:1087	The results revealed a dominant effect of CIP concentration over the alignment patterns induced by the magnetic field, which agrees with the behavior of the thermal diffusivity and thermal conductivity.
24274482	4	62	theme	CIP-agar	755:762	arg1	composites					764:773	the CIP-agar composites	751:773	the CIP-agar composites	751:773	The morphology of the CIP-agar composites with aligned and non-aligned CIP under magnetic field was also evaluated by scanning electron microscopy (SEM).
24274482	6	63	dep	promising	1234:1242	arg1	smart					1245:1249	smart	1245:1249	smart	1245:1249	Agar served as a perfect matrix to be used with CIP, and CIP-agar composites magnetically aligned at 20% CIP concentration can be considered as promising 'smart' material for hyperthermia treatments in the biomedical field.
24793172	5	0	theme	compressive	1499:1509	arg1	properties					1522:1531	compressive mechanical properties	1499:1531	compressive mechanical properties of cartilage matrix	1499:1551	Compressive modulus (E) and stress at 10% strain (σ10) of full scaffolds in wet state reached about 2.8MPa and 0.3MPa, respectively, and meanwhile, E and σ10 of layers inside hydrated scaffolds changed in a gradient-increased manner from the top layer to the bottom layer with significant differences between contiguous layers, which partially mimics compressive mechanical properties of cartilage matrix.
24793172	7	1	theme	promising	1872:1880	arg1	potential					1882:1890	promising potential	1872:1890	promising potential for applications	1872:1907	These results suggest that the presently developed collagen/CH-PCL/CS scaffolds have promising potential for applications in articular cartilage repair.
24793172	2	2	from	similarity	663:672	arg1	composition					701:711	the composition	697:711	the composition of main components	697:730	The ternary composites were designed in such a way that collagen content in the scaffolds decreased from the top layer to the bottom layer while the content of CH-PCL and CS altered in a reversed trend in order to reach partial similarity to cartilage matrix in the composition of main components.
24793172	1	3	theme	various	239:245	arg1	ratios					247:252	various ratios	239:252	various ratios	239:252	Collagen, chitosan-polycaprolactone (CH-PCL) copolymer with PCL content of around 40wt% and chondroitin sulfate (CS) were mixed together at various ratios to prepare collagen/CH-PCL/CS composites and the resulting composites were used to build stratified porous scaffolds that are potentially applicable for articular cartilage repair.
24793172	2	4	theme	collagen	491:498	arg1	content					500:506	collagen content	491:506	collagen content in the scaffolds	491:523	The ternary composites were designed in such a way that collagen content in the scaffolds decreased from the top layer to the bottom layer while the content of CH-PCL and CS altered in a reversed trend in order to reach partial similarity to cartilage matrix in the composition of main components.
24793172	1	5	theme	stratified	343:352	arg1	scaffolds					361:369	stratified porous scaffolds	343:369	stratified porous scaffolds that are potentially applicable for articular cartilage repair	343:432	Collagen, chitosan-polycaprolactone (CH-PCL) copolymer with PCL content of around 40wt% and chondroitin sulfate (CS) were mixed together at various ratios to prepare collagen/CH-PCL/CS composites and the resulting composites were used to build stratified porous scaffolds that are potentially applicable for articular cartilage repair.
24793172	2	6	theme	CH-PCL	595:600	arg1	content					584:590	the content	580:590	the content of CH-PCL and CS	580:607	The ternary composites were designed in such a way that collagen content in the scaffolds decreased from the top layer to the bottom layer while the content of CH-PCL and CS altered in a reversed trend in order to reach partial similarity to cartilage matrix in the composition of main components.
24793172	7	7	theme	collagen/CH-PCL/CS	1838:1855	arg1	scaffolds					1857:1865	the presently developed collagen/CH-PCL/CS scaffolds	1814:1865	the presently developed collagen/CH-PCL/CS scaffolds	1814:1865	These results suggest that the presently developed collagen/CH-PCL/CS scaffolds have promising potential for applications in articular cartilage repair.
24793172	4	8	theme	compressive	1124:1134	arg1	properties					1136:1145	well-defined elastic compressive properties	1103:1145	well-defined elastic compressive properties	1103:1145	Such produced scaffolds were further crosslinked using 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide under optimized conditions, and the obtained scaffolds showed well-defined elastic compressive properties.
24793172	1	9	theme	porous	354:359	arg1	scaffolds					361:369	stratified porous scaffolds	343:369	stratified porous scaffolds that are potentially applicable for articular cartilage repair	343:432	Collagen, chitosan-polycaprolactone (CH-PCL) copolymer with PCL content of around 40wt% and chondroitin sulfate (CS) were mixed together at various ratios to prepare collagen/CH-PCL/CS composites and the resulting composites were used to build stratified porous scaffolds that are potentially applicable for articular cartilage repair.
24793172	5	10	from	strain	1190:1195	arg1	E					1169:1169	E	1169:1169	E	1169:1169	Compressive modulus (E) and stress at 10% strain (σ10) of full scaffolds in wet state reached about 2.8MPa and 0.3MPa, respectively, and meanwhile, E and σ10 of layers inside hydrated scaffolds changed in a gradient-increased manner from the top layer to the bottom layer with significant differences between contiguous layers, which partially mimics compressive mechanical properties of cartilage matrix.
24793172	5	10	from	strain	1190:1195	arg1	stress					1176:1181	stress	1176:1181	stress at 10% strain (σ10) of full scaffolds in wet state	1176:1232	Compressive modulus (E) and stress at 10% strain (σ10) of full scaffolds in wet state reached about 2.8MPa and 0.3MPa, respectively, and meanwhile, E and σ10 of layers inside hydrated scaffolds changed in a gradient-increased manner from the top layer to the bottom layer with significant differences between contiguous layers, which partially mimics compressive mechanical properties of cartilage matrix.
24793172	5	10	from	strain	1190:1195	arg1	modulus					1160:1166	Compressive modulus	1148:1166	Compressive modulus (E)	1148:1170	Compressive modulus (E) and stress at 10% strain (σ10) of full scaffolds in wet state reached about 2.8MPa and 0.3MPa, respectively, and meanwhile, E and σ10 of layers inside hydrated scaffolds changed in a gradient-increased manner from the top layer to the bottom layer with significant differences between contiguous layers, which partially mimics compressive mechanical properties of cartilage matrix.
24793172	4	11	theme	elastic	1116:1122	arg1	properties					1136:1145	well-defined elastic compressive properties	1103:1145	well-defined elastic compressive properties	1103:1145	Such produced scaffolds were further crosslinked using 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide under optimized conditions, and the obtained scaffolds showed well-defined elastic compressive properties.
24793172	3	12	theme	deposition	828:837	arg1	technique					850:858	a low-temperature deposition processing technique	810:858	a low-temperature deposition processing technique	810:858	Porous structures inside collagen/CH-PCL/CS scaffolds were constructed using a low-temperature deposition processing technique and graded average pore-size and porosity for the scaffolds were established.
24793172	5	13	theme	gradient-increased	1355:1372	arg1	manner					1374:1379	a gradient-increased manner	1353:1379	a gradient-increased manner	1353:1379	Compressive modulus (E) and stress at 10% strain (σ10) of full scaffolds in wet state reached about 2.8MPa and 0.3MPa, respectively, and meanwhile, E and σ10 of layers inside hydrated scaffolds changed in a gradient-increased manner from the top layer to the bottom layer with significant differences between contiguous layers, which partially mimics compressive mechanical properties of cartilage matrix.
24793172	5	14	theme	contiguous	1457:1466	arg1	layers					1468:1473	contiguous layers	1457:1473	contiguous layers	1457:1473	Compressive modulus (E) and stress at 10% strain (σ10) of full scaffolds in wet state reached about 2.8MPa and 0.3MPa, respectively, and meanwhile, E and σ10 of layers inside hydrated scaffolds changed in a gradient-increased manner from the top layer to the bottom layer with significant differences between contiguous layers, which partially mimics compressive mechanical properties of cartilage matrix.
24793172	3	15	theme	processing	839:848	arg1	technique					850:858	a low-temperature deposition processing technique	810:858	a low-temperature deposition processing technique	810:858	Porous structures inside collagen/CH-PCL/CS scaffolds were constructed using a low-temperature deposition processing technique and graded average pore-size and porosity for the scaffolds were established.
24793172	6	16	theme	constructs	1601:1610	arg1	culture					1576:1582	in vitro culture	1567:1582	in vitro culture of cell-scaffold constructs	1567:1610	In addition, in vitro culture of cell-scaffold constructs exhibited that scaffolds were able to well support the ingrowth and migration of seeded cells, and cells also showed relatively uniform distribution throughout the scaffolds.
24793172	1	17	with	copolymer	144:152	arg1	content					163:169	PCL content	159:169	PCL content of around 40wt%	159:185	Collagen, chitosan-polycaprolactone (CH-PCL) copolymer with PCL content of around 40wt% and chondroitin sulfate (CS) were mixed together at various ratios to prepare collagen/CH-PCL/CS composites and the resulting composites were used to build stratified porous scaffolds that are potentially applicable for articular cartilage repair.
24793172	2	18	theme	reversed	622:629	arg1	trend					631:635	a reversed trend	620:635	a reversed trend in order to reach partial similarity to cartilage matrix in the composition of main components	620:730	The ternary composites were designed in such a way that collagen content in the scaffolds decreased from the top layer to the bottom layer while the content of CH-PCL and CS altered in a reversed trend in order to reach partial similarity to cartilage matrix in the composition of main components.
24793172	6	19	theme	in	1567:1568	arg1	culture					1576:1582	in vitro culture	1567:1582	in vitro culture of cell-scaffold constructs	1567:1610	In addition, in vitro culture of cell-scaffold constructs exhibited that scaffolds were able to well support the ingrowth and migration of seeded cells, and cells also showed relatively uniform distribution throughout the scaffolds.
24793172	4	20	theme	1-ethyl-3-	993:1002	arg1	carbodiimide					1028:1039	1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide	993:1039	1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide under optimized conditions	993:1066	Such produced scaffolds were further crosslinked using 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide under optimized conditions, and the obtained scaffolds showed well-defined elastic compressive properties.
24793172	6	21	theme	uniform	1740:1746	arg1	distribution					1748:1759	relatively uniform distribution	1729:1759	relatively uniform distribution	1729:1759	In addition, in vitro culture of cell-scaffold constructs exhibited that scaffolds were able to well support the ingrowth and migration of seeded cells, and cells also showed relatively uniform distribution throughout the scaffolds.
24793172	4	22	theme	optimized	1047:1055	arg1	conditions					1057:1066	optimized conditions	1047:1066	optimized conditions	1047:1066	Such produced scaffolds were further crosslinked using 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide under optimized conditions, and the obtained scaffolds showed well-defined elastic compressive properties.
24793172	5	23	theme	bottom	1407:1412	arg1	layer					1414:1418	the bottom layer	1403:1418	the bottom layer with significant differences between contiguous layers, which partially mimics compressive mechanical properties of cartilage matrix	1403:1551	Compressive modulus (E) and stress at 10% strain (σ10) of full scaffolds in wet state reached about 2.8MPa and 0.3MPa, respectively, and meanwhile, E and σ10 of layers inside hydrated scaffolds changed in a gradient-increased manner from the top layer to the bottom layer with significant differences between contiguous layers, which partially mimics compressive mechanical properties of cartilage matrix.
24793172	6	24	dep	in	1567:1568	arg1	vitro					1570:1574	vitro	1570:1574	vitro	1570:1574	In addition, in vitro culture of cell-scaffold constructs exhibited that scaffolds were able to well support the ingrowth and migration of seeded cells, and cells also showed relatively uniform distribution throughout the scaffolds.
24793172	7	25	theme	developed	1828:1836	arg1	scaffolds					1857:1865	the presently developed collagen/CH-PCL/CS scaffolds	1814:1865	the presently developed collagen/CH-PCL/CS scaffolds	1814:1865	These results suggest that the presently developed collagen/CH-PCL/CS scaffolds have promising potential for applications in articular cartilage repair.
24793172	1	26	theme	%	185:185	arg1	content					163:169	PCL content	159:169	PCL content of around 40wt%	159:185	Collagen, chitosan-polycaprolactone (CH-PCL) copolymer with PCL content of around 40wt% and chondroitin sulfate (CS) were mixed together at various ratios to prepare collagen/CH-PCL/CS composites and the resulting composites were used to build stratified porous scaffolds that are potentially applicable for articular cartilage repair.
24793172	0	27	theme	matrix	91:96	arg1	properties					57:66	compressive mechanical properties	34:66	compressive mechanical properties of articular cartilage matrix	34:96	Designed composites for mimicking compressive mechanical properties of articular cartilage matrix.
24793172	2	28	theme	partial	655:661	arg1	similarity					663:672	partial similarity	655:672	partial similarity to cartilage matrix in the composition of main components	655:730	The ternary composites were designed in such a way that collagen content in the scaffolds decreased from the top layer to the bottom layer while the content of CH-PCL and CS altered in a reversed trend in order to reach partial similarity to cartilage matrix in the composition of main components.
24793172	2	29	theme	components	721:730	arg1	composition					701:711	the composition	697:711	the composition of main components	697:730	The ternary composites were designed in such a way that collagen content in the scaffolds decreased from the top layer to the bottom layer while the content of CH-PCL and CS altered in a reversed trend in order to reach partial similarity to cartilage matrix in the composition of main components.
24793172	5	30	theme	10	1186:1187	arg1	%					1188:1188	%	1188:1188	%	1188:1188	Compressive modulus (E) and stress at 10% strain (σ10) of full scaffolds in wet state reached about 2.8MPa and 0.3MPa, respectively, and meanwhile, E and σ10 of layers inside hydrated scaffolds changed in a gradient-increased manner from the top layer to the bottom layer with significant differences between contiguous layers, which partially mimics compressive mechanical properties of cartilage matrix.
24793172	1	31	theme	collagen/CH-PCL/CS	265:282	arg1	composites					284:293	collagen/CH-PCL/CS composites	265:293	collagen/CH-PCL/CS composites	265:293	Collagen, chitosan-polycaprolactone (CH-PCL) copolymer with PCL content of around 40wt% and chondroitin sulfate (CS) were mixed together at various ratios to prepare collagen/CH-PCL/CS composites and the resulting composites were used to build stratified porous scaffolds that are potentially applicable for articular cartilage repair.
24793172	2	32	theme	CS	606:607	arg1	content					584:590	the content	580:590	the content of CH-PCL and CS	580:607	The ternary composites were designed in such a way that collagen content in the scaffolds decreased from the top layer to the bottom layer while the content of CH-PCL and CS altered in a reversed trend in order to reach partial similarity to cartilage matrix in the composition of main components.
24793172	6	33	dep	ingrowth	1667:1674	arg1	the					1663:1665	the	1663:1665	the	1663:1665	In addition, in vitro culture of cell-scaffold constructs exhibited that scaffolds were able to well support the ingrowth and migration of seeded cells, and cells also showed relatively uniform distribution throughout the scaffolds.
24793172	1	34	theme	chondroitin	191:201	arg1	CS					212:213	CS	212:213	CS	212:213	Collagen, chitosan-polycaprolactone (CH-PCL) copolymer with PCL content of around 40wt% and chondroitin sulfate (CS) were mixed together at various ratios to prepare collagen/CH-PCL/CS composites and the resulting composites were used to build stratified porous scaffolds that are potentially applicable for articular cartilage repair.
24793172	1	34	theme	chondroitin	191:201	arg1	sulfate					203:209	chondroitin sulfate	191:209	chondroitin sulfate (CS)	191:214	Collagen, chitosan-polycaprolactone (CH-PCL) copolymer with PCL content of around 40wt% and chondroitin sulfate (CS) were mixed together at various ratios to prepare collagen/CH-PCL/CS composites and the resulting composites were used to build stratified porous scaffolds that are potentially applicable for articular cartilage repair.
24793172	5	35	theme	full	1206:1209	arg1	scaffolds					1211:1219	full scaffolds	1206:1219	full scaffolds	1206:1219	Compressive modulus (E) and stress at 10% strain (σ10) of full scaffolds in wet state reached about 2.8MPa and 0.3MPa, respectively, and meanwhile, E and σ10 of layers inside hydrated scaffolds changed in a gradient-increased manner from the top layer to the bottom layer with significant differences between contiguous layers, which partially mimics compressive mechanical properties of cartilage matrix.
24793172	7	36	contain	have	1867:1870	arg2	potential					1882:1890	promising potential	1872:1890	promising potential for applications	1872:1907	These results suggest that the presently developed collagen/CH-PCL/CS scaffolds have promising potential for applications in articular cartilage repair.
24793172	7	36	contain	have	1867:1870	arg1	scaffolds					1857:1865	the presently developed collagen/CH-PCL/CS scaffolds	1814:1865	the presently developed collagen/CH-PCL/CS scaffolds	1814:1865	These results suggest that the presently developed collagen/CH-PCL/CS scaffolds have promising potential for applications in articular cartilage repair.
24793172	5	37	theme	significant	1425:1435	arg1	differences					1437:1447	significant differences	1425:1447	significant differences between contiguous layers, which partially mimics compressive mechanical properties of cartilage matrix	1425:1551	Compressive modulus (E) and stress at 10% strain (σ10) of full scaffolds in wet state reached about 2.8MPa and 0.3MPa, respectively, and meanwhile, E and σ10 of layers inside hydrated scaffolds changed in a gradient-increased manner from the top layer to the bottom layer with significant differences between contiguous layers, which partially mimics compressive mechanical properties of cartilage matrix.
24793172	1	38	theme	chitosan-polycaprolactone	109:133	arg1	copolymer					144:152	chitosan-polycaprolactone (CH-PCL) copolymer	109:152	chitosan-polycaprolactone (CH-PCL) copolymer with PCL content of around 40wt%	109:185	Collagen, chitosan-polycaprolactone (CH-PCL) copolymer with PCL content of around 40wt% and chondroitin sulfate (CS) were mixed together at various ratios to prepare collagen/CH-PCL/CS composites and the resulting composites were used to build stratified porous scaffolds that are potentially applicable for articular cartilage repair.
24793172	1	38	theme	chitosan-polycaprolactone	109:133	arg1	Collagen					99:106	Collagen	99:106	Collagen	99:106	Collagen, chitosan-polycaprolactone (CH-PCL) copolymer with PCL content of around 40wt% and chondroitin sulfate (CS) were mixed together at various ratios to prepare collagen/CH-PCL/CS composites and the resulting composites were used to build stratified porous scaffolds that are potentially applicable for articular cartilage repair.
24793172	5	39	theme	layers	1309:1314	arg1	E					1296:1296	E	1296:1296	E	1296:1296	Compressive modulus (E) and stress at 10% strain (σ10) of full scaffolds in wet state reached about 2.8MPa and 0.3MPa, respectively, and meanwhile, E and σ10 of layers inside hydrated scaffolds changed in a gradient-increased manner from the top layer to the bottom layer with significant differences between contiguous layers, which partially mimics compressive mechanical properties of cartilage matrix.
24793172	5	39	theme	layers	1309:1314	arg1	meanwhile					1285:1293	meanwhile	1285:1293	meanwhile	1285:1293	Compressive modulus (E) and stress at 10% strain (σ10) of full scaffolds in wet state reached about 2.8MPa and 0.3MPa, respectively, and meanwhile, E and σ10 of layers inside hydrated scaffolds changed in a gradient-increased manner from the top layer to the bottom layer with significant differences between contiguous layers, which partially mimics compressive mechanical properties of cartilage matrix.
24793172	5	39	theme	layers	1309:1314	arg1	σ10					1302:1304	σ10	1302:1304	σ10 of layers	1302:1314	Compressive modulus (E) and stress at 10% strain (σ10) of full scaffolds in wet state reached about 2.8MPa and 0.3MPa, respectively, and meanwhile, E and σ10 of layers inside hydrated scaffolds changed in a gradient-increased manner from the top layer to the bottom layer with significant differences between contiguous layers, which partially mimics compressive mechanical properties of cartilage matrix.
24793172	2	40	theme	ternary	439:445	arg1	composites					447:456	The ternary composites	435:456	The ternary composites	435:456	The ternary composites were designed in such a way that collagen content in the scaffolds decreased from the top layer to the bottom layer while the content of CH-PCL and CS altered in a reversed trend in order to reach partial similarity to cartilage matrix in the composition of main components.
24793172	4	41	theme	obtained	1077:1084	arg1	scaffolds					1086:1094	the obtained scaffolds	1073:1094	the obtained scaffolds	1073:1094	Such produced scaffolds were further crosslinked using 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide under optimized conditions, and the obtained scaffolds showed well-defined elastic compressive properties.
24793172	5	42	theme	scaffolds	1211:1219	arg1	σ10					1198:1200	σ10	1198:1200	σ10	1198:1200	Compressive modulus (E) and stress at 10% strain (σ10) of full scaffolds in wet state reached about 2.8MPa and 0.3MPa, respectively, and meanwhile, E and σ10 of layers inside hydrated scaffolds changed in a gradient-increased manner from the top layer to the bottom layer with significant differences between contiguous layers, which partially mimics compressive mechanical properties of cartilage matrix.
24793172	5	42	theme	scaffolds	1211:1219	arg1	strain					1190:1195	10% strain	1186:1195	10% strain (σ10) of full scaffolds	1186:1219	Compressive modulus (E) and stress at 10% strain (σ10) of full scaffolds in wet state reached about 2.8MPa and 0.3MPa, respectively, and meanwhile, E and σ10 of layers inside hydrated scaffolds changed in a gradient-increased manner from the top layer to the bottom layer with significant differences between contiguous layers, which partially mimics compressive mechanical properties of cartilage matrix.
24793172	4	43	theme	produced	943:950	arg1	scaffolds					952:960	Such produced scaffolds	938:960	Such produced scaffolds	938:960	Such produced scaffolds were further crosslinked using 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide under optimized conditions, and the obtained scaffolds showed well-defined elastic compressive properties.
24793172	2	44	from	content	500:506	arg1	scaffolds					515:523	the scaffolds	511:523	the scaffolds	511:523	The ternary composites were designed in such a way that collagen content in the scaffolds decreased from the top layer to the bottom layer while the content of CH-PCL and CS altered in a reversed trend in order to reach partial similarity to cartilage matrix in the composition of main components.
24793172	5	45	theme	mechanical	1511:1520	arg1	properties					1522:1531	compressive mechanical properties	1499:1531	compressive mechanical properties of cartilage matrix	1499:1551	Compressive modulus (E) and stress at 10% strain (σ10) of full scaffolds in wet state reached about 2.8MPa and 0.3MPa, respectively, and meanwhile, E and σ10 of layers inside hydrated scaffolds changed in a gradient-increased manner from the top layer to the bottom layer with significant differences between contiguous layers, which partially mimics compressive mechanical properties of cartilage matrix.
24793172	4	46	theme	aminopropyl	1015:1025	arg1	carbodiimide					1028:1039	1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide	993:1039	1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide under optimized conditions	993:1066	Such produced scaffolds were further crosslinked using 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide under optimized conditions, and the obtained scaffolds showed well-defined elastic compressive properties.
24793172	0	47	theme	mechanical	46:55	arg1	properties					57:66	compressive mechanical properties	34:66	compressive mechanical properties of articular cartilage matrix	34:96	Designed composites for mimicking compressive mechanical properties of articular cartilage matrix.
24793172	5	48	with	layer	1414:1418	arg1	differences					1437:1447	significant differences	1425:1447	significant differences between contiguous layers, which partially mimics compressive mechanical properties of cartilage matrix	1425:1551	Compressive modulus (E) and stress at 10% strain (σ10) of full scaffolds in wet state reached about 2.8MPa and 0.3MPa, respectively, and meanwhile, E and σ10 of layers inside hydrated scaffolds changed in a gradient-increased manner from the top layer to the bottom layer with significant differences between contiguous layers, which partially mimics compressive mechanical properties of cartilage matrix.
24793172	3	49	theme	Porous	733:738	arg1	structures					740:749	Porous structures	733:749	Porous structures inside collagen/CH-PCL/CS scaffolds	733:785	Porous structures inside collagen/CH-PCL/CS scaffolds were constructed using a low-temperature deposition processing technique and graded average pore-size and porosity for the scaffolds were established.
24793172	5	50	theme	hydrated	1323:1330	arg1	scaffolds					1332:1340	hydrated scaffolds	1323:1340	hydrated scaffolds changed in a gradient-increased manner from the top layer to the bottom layer with significant differences between contiguous layers, which partially mimics compressive mechanical properties of cartilage matrix	1323:1551	Compressive modulus (E) and stress at 10% strain (σ10) of full scaffolds in wet state reached about 2.8MPa and 0.3MPa, respectively, and meanwhile, E and σ10 of layers inside hydrated scaffolds changed in a gradient-increased manner from the top layer to the bottom layer with significant differences between contiguous layers, which partially mimics compressive mechanical properties of cartilage matrix.
24793172	0	51	theme	compressive	34:44	arg1	properties					57:66	compressive mechanical properties	34:66	compressive mechanical properties of articular cartilage matrix	34:96	Designed composites for mimicking compressive mechanical properties of articular cartilage matrix.
24793172	1	52	theme	resulting	303:311	arg1	composites					313:322	the resulting composites	299:322	the resulting composites	299:322	Collagen, chitosan-polycaprolactone (CH-PCL) copolymer with PCL content of around 40wt% and chondroitin sulfate (CS) were mixed together at various ratios to prepare collagen/CH-PCL/CS composites and the resulting composites were used to build stratified porous scaffolds that are potentially applicable for articular cartilage repair.
24793172	2	53	theme	top	544:546	arg1	layer					548:552	the top layer	540:552	the top layer	540:552	The ternary composites were designed in such a way that collagen content in the scaffolds decreased from the top layer to the bottom layer while the content of CH-PCL and CS altered in a reversed trend in order to reach partial similarity to cartilage matrix in the composition of main components.
24793172	1	54	theme	articular	407:415	arg1	repair					427:432	articular cartilage repair	407:432	articular cartilage repair	407:432	Collagen, chitosan-polycaprolactone (CH-PCL) copolymer with PCL content of around 40wt% and chondroitin sulfate (CS) were mixed together at various ratios to prepare collagen/CH-PCL/CS composites and the resulting composites were used to build stratified porous scaffolds that are potentially applicable for articular cartilage repair.
24793172	5	55	theme	top	1390:1392	arg1	layer					1394:1398	the top layer	1386:1398	the top layer to the bottom layer with significant differences between contiguous layers, which partially mimics compressive mechanical properties of cartilage matrix	1386:1551	Compressive modulus (E) and stress at 10% strain (σ10) of full scaffolds in wet state reached about 2.8MPa and 0.3MPa, respectively, and meanwhile, E and σ10 of layers inside hydrated scaffolds changed in a gradient-increased manner from the top layer to the bottom layer with significant differences between contiguous layers, which partially mimics compressive mechanical properties of cartilage matrix.
24793172	5	56	from	stress	1176:1181	arg1	state					1228:1232	wet state	1224:1232	wet state	1224:1232	Compressive modulus (E) and stress at 10% strain (σ10) of full scaffolds in wet state reached about 2.8MPa and 0.3MPa, respectively, and meanwhile, E and σ10 of layers inside hydrated scaffolds changed in a gradient-increased manner from the top layer to the bottom layer with significant differences between contiguous layers, which partially mimics compressive mechanical properties of cartilage matrix.
24793172	5	57	theme	cartilage	1536:1544	arg1	matrix					1546:1551	cartilage matrix	1536:1551	cartilage matrix	1536:1551	Compressive modulus (E) and stress at 10% strain (σ10) of full scaffolds in wet state reached about 2.8MPa and 0.3MPa, respectively, and meanwhile, E and σ10 of layers inside hydrated scaffolds changed in a gradient-increased manner from the top layer to the bottom layer with significant differences between contiguous layers, which partially mimics compressive mechanical properties of cartilage matrix.
24793172	1	58	theme	cartilage	417:425	arg1	repair					427:432	articular cartilage repair	407:432	articular cartilage repair	407:432	Collagen, chitosan-polycaprolactone (CH-PCL) copolymer with PCL content of around 40wt% and chondroitin sulfate (CS) were mixed together at various ratios to prepare collagen/CH-PCL/CS composites and the resulting composites were used to build stratified porous scaffolds that are potentially applicable for articular cartilage repair.
24793172	7	59	theme	cartilage	1922:1930	arg1	repair					1932:1937	articular cartilage repair	1912:1937	articular cartilage repair	1912:1937	These results suggest that the presently developed collagen/CH-PCL/CS scaffolds have promising potential for applications in articular cartilage repair.
24793172	1	60	theme	CH-PCL	136:141	arg1	copolymer					144:152	chitosan-polycaprolactone (CH-PCL) copolymer	109:152	chitosan-polycaprolactone (CH-PCL) copolymer with PCL content of around 40wt%	109:185	Collagen, chitosan-polycaprolactone (CH-PCL) copolymer with PCL content of around 40wt% and chondroitin sulfate (CS) were mixed together at various ratios to prepare collagen/CH-PCL/CS composites and the resulting composites were used to build stratified porous scaffolds that are potentially applicable for articular cartilage repair.
24793172	1	60	theme	CH-PCL	136:141	arg1	Collagen					99:106	Collagen	99:106	Collagen	99:106	Collagen, chitosan-polycaprolactone (CH-PCL) copolymer with PCL content of around 40wt% and chondroitin sulfate (CS) were mixed together at various ratios to prepare collagen/CH-PCL/CS composites and the resulting composites were used to build stratified porous scaffolds that are potentially applicable for articular cartilage repair.
24793172	5	61	theme	%	1188:1188	arg1	σ10					1198:1200	σ10	1198:1200	σ10	1198:1200	Compressive modulus (E) and stress at 10% strain (σ10) of full scaffolds in wet state reached about 2.8MPa and 0.3MPa, respectively, and meanwhile, E and σ10 of layers inside hydrated scaffolds changed in a gradient-increased manner from the top layer to the bottom layer with significant differences between contiguous layers, which partially mimics compressive mechanical properties of cartilage matrix.
24793172	5	61	theme	%	1188:1188	arg1	strain					1190:1195	10% strain	1186:1195	10% strain (σ10) of full scaffolds	1186:1219	Compressive modulus (E) and stress at 10% strain (σ10) of full scaffolds in wet state reached about 2.8MPa and 0.3MPa, respectively, and meanwhile, E and σ10 of layers inside hydrated scaffolds changed in a gradient-increased manner from the top layer to the bottom layer with significant differences between contiguous layers, which partially mimics compressive mechanical properties of cartilage matrix.
24793172	5	62	theme	matrix	1546:1551	arg1	properties					1522:1531	compressive mechanical properties	1499:1531	compressive mechanical properties of cartilage matrix	1499:1551	Compressive modulus (E) and stress at 10% strain (σ10) of full scaffolds in wet state reached about 2.8MPa and 0.3MPa, respectively, and meanwhile, E and σ10 of layers inside hydrated scaffolds changed in a gradient-increased manner from the top layer to the bottom layer with significant differences between contiguous layers, which partially mimics compressive mechanical properties of cartilage matrix.
24793172	4	63	theme	3-dimethyl	1004:1013	arg1	carbodiimide					1028:1039	1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide	993:1039	1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide under optimized conditions	993:1066	Such produced scaffolds were further crosslinked using 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide under optimized conditions, and the obtained scaffolds showed well-defined elastic compressive properties.
24793172	2	64	theme	cartilage	677:685	arg1	matrix					687:692	cartilage matrix	677:692	cartilage matrix	677:692	The ternary composites were designed in such a way that collagen content in the scaffolds decreased from the top layer to the bottom layer while the content of CH-PCL and CS altered in a reversed trend in order to reach partial similarity to cartilage matrix in the composition of main components.
24793172	5	65	theme	Compressive	1148:1158	arg1	E					1169:1169	E	1169:1169	E	1169:1169	Compressive modulus (E) and stress at 10% strain (σ10) of full scaffolds in wet state reached about 2.8MPa and 0.3MPa, respectively, and meanwhile, E and σ10 of layers inside hydrated scaffolds changed in a gradient-increased manner from the top layer to the bottom layer with significant differences between contiguous layers, which partially mimics compressive mechanical properties of cartilage matrix.
24793172	5	65	theme	Compressive	1148:1158	arg1	modulus					1160:1166	Compressive modulus	1148:1166	Compressive modulus (E)	1148:1170	Compressive modulus (E) and stress at 10% strain (σ10) of full scaffolds in wet state reached about 2.8MPa and 0.3MPa, respectively, and meanwhile, E and σ10 of layers inside hydrated scaffolds changed in a gradient-increased manner from the top layer to the bottom layer with significant differences between contiguous layers, which partially mimics compressive mechanical properties of cartilage matrix.
24793172	3	66	theme	collagen/CH-PCL/CS	758:775	arg1	scaffolds					777:785	collagen/CH-PCL/CS scaffolds	758:785	collagen/CH-PCL/CS scaffolds	758:785	Porous structures inside collagen/CH-PCL/CS scaffolds were constructed using a low-temperature deposition processing technique and graded average pore-size and porosity for the scaffolds were established.
24793172	4	67	theme	Such	938:941	arg1	scaffolds					952:960	Such produced scaffolds	938:960	Such produced scaffolds	938:960	Such produced scaffolds were further crosslinked using 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide under optimized conditions, and the obtained scaffolds showed well-defined elastic compressive properties.
24793172	6	68	theme	cells	1700:1704	arg1	ingrowth					1667:1674	ingrowth	1667:1674	ingrowth	1667:1674	In addition, in vitro culture of cell-scaffold constructs exhibited that scaffolds were able to well support the ingrowth and migration of seeded cells, and cells also showed relatively uniform distribution throughout the scaffolds.
24793172	6	68	theme	cells	1700:1704	arg1	migration					1680:1688	migration	1680:1688	migration	1680:1688	In addition, in vitro culture of cell-scaffold constructs exhibited that scaffolds were able to well support the ingrowth and migration of seeded cells, and cells also showed relatively uniform distribution throughout the scaffolds.
24793172	2	69	theme	bottom	561:566	arg1	layer					568:572	the bottom layer	557:572	the bottom layer	557:572	The ternary composites were designed in such a way that collagen content in the scaffolds decreased from the top layer to the bottom layer while the content of CH-PCL and CS altered in a reversed trend in order to reach partial similarity to cartilage matrix in the composition of main components.
24793172	6	70	theme	seeded	1693:1698	arg1	cells					1700:1704	seeded cells	1693:1704	seeded cells	1693:1704	In addition, in vitro culture of cell-scaffold constructs exhibited that scaffolds were able to well support the ingrowth and migration of seeded cells, and cells also showed relatively uniform distribution throughout the scaffolds.
24793172	7	71	theme	articular	1912:1920	arg1	repair					1932:1937	articular cartilage repair	1912:1937	articular cartilage repair	1912:1937	These results suggest that the presently developed collagen/CH-PCL/CS scaffolds have promising potential for applications in articular cartilage repair.
24793172	2	72	theme	main	716:719	arg1	components					721:730	main components	716:730	main components	716:730	The ternary composites were designed in such a way that collagen content in the scaffolds decreased from the top layer to the bottom layer while the content of CH-PCL and CS altered in a reversed trend in order to reach partial similarity to cartilage matrix in the composition of main components.
24793172	3	73	theme	graded	864:869	arg1	pore-size					879:887	graded average pore-size	864:887	graded average pore-size	864:887	Porous structures inside collagen/CH-PCL/CS scaffolds were constructed using a low-temperature deposition processing technique and graded average pore-size and porosity for the scaffolds were established.
24793172	3	74	theme	low-temperature	812:826	arg1	technique					850:858	a low-temperature deposition processing technique	810:858	a low-temperature deposition processing technique	810:858	Porous structures inside collagen/CH-PCL/CS scaffolds were constructed using a low-temperature deposition processing technique and graded average pore-size and porosity for the scaffolds were established.
24793172	0	75	theme	cartilage	81:89	arg1	matrix					91:96	articular cartilage matrix	71:96	articular cartilage matrix	71:96	Designed composites for mimicking compressive mechanical properties of articular cartilage matrix.
24793172	6	76	theme	cell-scaffold	1587:1599	arg1	constructs					1601:1610	cell-scaffold constructs	1587:1610	cell-scaffold constructs	1587:1610	In addition, in vitro culture of cell-scaffold constructs exhibited that scaffolds were able to well support the ingrowth and migration of seeded cells, and cells also showed relatively uniform distribution throughout the scaffolds.
24793172	1	77	theme	PCL	159:161	arg1	content					163:169	PCL content	159:169	PCL content of around 40wt%	159:185	Collagen, chitosan-polycaprolactone (CH-PCL) copolymer with PCL content of around 40wt% and chondroitin sulfate (CS) were mixed together at various ratios to prepare collagen/CH-PCL/CS composites and the resulting composites were used to build stratified porous scaffolds that are potentially applicable for articular cartilage repair.
24793172	3	78	theme	average	871:877	arg1	pore-size					879:887	graded average pore-size	864:887	graded average pore-size	864:887	Porous structures inside collagen/CH-PCL/CS scaffolds were constructed using a low-temperature deposition processing technique and graded average pore-size and porosity for the scaffolds were established.
24793172	0	79	theme	articular	71:79	arg1	matrix					91:96	articular cartilage matrix	71:96	articular cartilage matrix	71:96	Designed composites for mimicking compressive mechanical properties of articular cartilage matrix.
24793172	1	80	used	used	329:332	arg2	composites					313:322	the resulting composites	299:322	the resulting composites	299:322	Collagen, chitosan-polycaprolactone (CH-PCL) copolymer with PCL content of around 40wt% and chondroitin sulfate (CS) were mixed together at various ratios to prepare collagen/CH-PCL/CS composites and the resulting composites were used to build stratified porous scaffolds that are potentially applicable for articular cartilage repair.
24793172	5	81	theme	wet	1224:1226	arg1	state					1228:1232	wet state	1224:1232	wet state	1224:1232	Compressive modulus (E) and stress at 10% strain (σ10) of full scaffolds in wet state reached about 2.8MPa and 0.3MPa, respectively, and meanwhile, E and σ10 of layers inside hydrated scaffolds changed in a gradient-increased manner from the top layer to the bottom layer with significant differences between contiguous layers, which partially mimics compressive mechanical properties of cartilage matrix.
24793172	4	82	theme	well-defined	1103:1114	arg1	properties					1136:1145	well-defined elastic compressive properties	1103:1145	well-defined elastic compressive properties	1103:1145	Such produced scaffolds were further crosslinked using 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide under optimized conditions, and the obtained scaffolds showed well-defined elastic compressive properties.
24793172	5	83	from	modulus	1160:1166	arg1	state					1228:1232	wet state	1224:1232	wet state	1224:1232	Compressive modulus (E) and stress at 10% strain (σ10) of full scaffolds in wet state reached about 2.8MPa and 0.3MPa, respectively, and meanwhile, E and σ10 of layers inside hydrated scaffolds changed in a gradient-increased manner from the top layer to the bottom layer with significant differences between contiguous layers, which partially mimics compressive mechanical properties of cartilage matrix.
24877644	2	0	from	Effect	201:206	arg1	properties					238:247	the properties	234:247	the properties of polyester composites	234:271	Effect of isora nanocellulose on the properties of polyester composites has been studied in detail.
24877644	3	1	theme	resin	349:353	arg1	suspensions					355:365	unsaturated polyester resin suspensions	327:365	unsaturated polyester resin suspensions containing various amounts (0.5, 1 and 3wt%) of nanocellulose	327:427	Rheological properties of unsaturated polyester resin suspensions containing various amounts (0.5, 1 and 3wt%) of nanocellulose were investigated by oscillatory rheometer with parallel plate geometry.
24877644	3	2	contain	containing	367:376	arg2	nanocellulose					415:427	nanocellulose	415:427	nanocellulose	415:427	Rheological properties of unsaturated polyester resin suspensions containing various amounts (0.5, 1 and 3wt%) of nanocellulose were investigated by oscillatory rheometer with parallel plate geometry.
24877644	3	2	contain	containing	367:376	arg2	%					409:409	0.5, 1 and 3wt%	395:409	0.5, 1 and 3wt%	395:409	Rheological properties of unsaturated polyester resin suspensions containing various amounts (0.5, 1 and 3wt%) of nanocellulose were investigated by oscillatory rheometer with parallel plate geometry.
24877644	3	2	contain	containing	367:376	arg1	suspensions					355:365	unsaturated polyester resin suspensions	327:365	unsaturated polyester resin suspensions containing various amounts (0.5, 1 and 3wt%) of nanocellulose	327:427	Rheological properties of unsaturated polyester resin suspensions containing various amounts (0.5, 1 and 3wt%) of nanocellulose were investigated by oscillatory rheometer with parallel plate geometry.
24877644	3	2	contain	containing	367:376	arg2	amounts					386:392	various amounts	378:392	various amounts (0.5, 1 and 3wt%) of nanocellulose	378:427	Rheological properties of unsaturated polyester resin suspensions containing various amounts (0.5, 1 and 3wt%) of nanocellulose were investigated by oscillatory rheometer with parallel plate geometry.
24877644	5	3	theme	NC	669:670	arg1	suspensions					693:703	NC reinforced polyester suspensions	669:703	NC reinforced polyester suspensions	669:703	NC reinforced polyester suspensions showed shear thinning behaviour initially and at higher shear rates they showed Newtonian behaviour.
24877644	3	4	theme	suspensions	355:365	arg1	properties					313:322	Rheological properties	301:322	Rheological properties of unsaturated polyester resin suspensions containing various amounts (0.5, 1 and 3wt%) of nanocellulose	301:427	Rheological properties of unsaturated polyester resin suspensions containing various amounts (0.5, 1 and 3wt%) of nanocellulose were investigated by oscillatory rheometer with parallel plate geometry.
24877644	5	5	theme	Newtonian	785:793	arg1	behaviour					795:803	Newtonian behaviour	785:803	Newtonian behaviour	785:803	NC reinforced polyester suspensions showed shear thinning behaviour initially and at higher shear rates they showed Newtonian behaviour.
24877644	9	6	theme	glass	1202:1206	arg1	transition					1208:1217	Maximum glass transition	1194:1217	Maximum glass transition temperature	1194:1229	Maximum glass transition temperature is obtained for 0.5wt% NC reinforced composite, which showed an increase of 10°C than neat resin.
24877644	1	7	theme	mechanical	174:183	arg1	process					192:198	mechanical mixing process	174:198	mechanical mixing process	174:198	Nanocellulose (NC) reinforced unsaturated polyester (UPR) composites were prepared by mechanical mixing process.
24877644	7	8	dep	polyester	969:977	arg1	respect					949:955	respect	949:955	respect	949:955	With respect to the neat polyester the percentage increase in tensile strength of 0.5wt% NC reinforced composite is 57%.
24877644	9	9	theme	neat	1317:1320	arg1	resin					1322:1326	neat resin	1317:1326	neat resin	1317:1326	Maximum glass transition temperature is obtained for 0.5wt% NC reinforced composite, which showed an increase of 10°C than neat resin.
24877644	8	10	theme	Optical	1065:1071	arg1	studies					1102:1108	Optical and atomic force microscopic studies	1065:1108	Optical and atomic force microscopic studies	1065:1108	Optical and atomic force microscopic studies confirmed that the dispersion state of NC within the polyester matrix was adequate.
24877644	7	11	theme	percentage	983:992	arg1	increase					994:1001	the percentage increase	979:1001	the percentage increase in tensile strength of 0.5wt% NC reinforced composite	979:1055	With respect to the neat polyester the percentage increase in tensile strength of 0.5wt% NC reinforced composite is 57%.
24877644	7	11	theme	percentage	983:992	arg1	%					1062:1062	57%	1060:1062	57%	1060:1062	With respect to the neat polyester the percentage increase in tensile strength of 0.5wt% NC reinforced composite is 57%.
24877644	9	12	theme	Maximum	1194:1200	arg1	transition					1208:1217	Maximum glass transition	1194:1217	Maximum glass transition temperature	1194:1229	Maximum glass transition temperature is obtained for 0.5wt% NC reinforced composite, which showed an increase of 10°C than neat resin.
24877644	4	13	theme	cure	654:657	arg1	reaction					659:666	cure reaction	654:666	cure reaction	654:666	Analysis of curing revealed that the time required for gelation in NC filled UPR is lower than neat resin, which describe the catalytic action of NC on cure reaction.
24877644	5	14	theme	shear	712:716	arg1	behaviour					727:735	shear thinning behaviour	712:735	shear thinning behaviour	712:735	NC reinforced polyester suspensions showed shear thinning behaviour initially and at higher shear rates they showed Newtonian behaviour.
24877644	6	15	theme	impact	818:823	arg1	properties					825:834	Tensile and impact properties	806:834	properties	825:834	Tensile and impact properties showed superior behaviour revealing improved interfacial bonding between nanofiller and the polymer matrix.
24877644	4	16	theme	neat	597:600	arg1	resin					602:606	neat resin	597:606	neat resin	597:606	Analysis of curing revealed that the time required for gelation in NC filled UPR is lower than neat resin, which describe the catalytic action of NC on cure reaction.
24877644	6	17	theme	polymer	928:934	arg1	matrix					936:941	the polymer matrix	924:941	the polymer matrix	924:941	Tensile and impact properties showed superior behaviour revealing improved interfacial bonding between nanofiller and the polymer matrix.
24877644	8	18	theme	atomic	1077:1082	arg1	studies					1102:1108	Optical and atomic force microscopic studies	1065:1108	Optical and atomic force microscopic studies	1065:1108	Optical and atomic force microscopic studies confirmed that the dispersion state of NC within the polyester matrix was adequate.
24877644	1	19	theme	mixing	185:190	arg1	process					192:198	mechanical mixing process	174:198	mechanical mixing process	174:198	Nanocellulose (NC) reinforced unsaturated polyester (UPR) composites were prepared by mechanical mixing process.
24877644	5	20	theme	higher	754:759	arg1	rates					767:771	higher shear rates	754:771	higher shear rates	754:771	NC reinforced polyester suspensions showed shear thinning behaviour initially and at higher shear rates they showed Newtonian behaviour.
24877644	4	21	theme	curing	514:519	arg1	Analysis					502:509	Analysis	502:509	Analysis of curing	502:519	Analysis of curing revealed that the time required for gelation in NC filled UPR is lower than neat resin, which describe the catalytic action of NC on cure reaction.
24877644	3	22	theme	nanocellulose	415:427	arg1	%					409:409	0.5, 1 and 3wt%	395:409	0.5, 1 and 3wt%	395:409	Rheological properties of unsaturated polyester resin suspensions containing various amounts (0.5, 1 and 3wt%) of nanocellulose were investigated by oscillatory rheometer with parallel plate geometry.
24877644	3	22	theme	nanocellulose	415:427	arg1	nanocellulose					415:427	nanocellulose	415:427	nanocellulose	415:427	Rheological properties of unsaturated polyester resin suspensions containing various amounts (0.5, 1 and 3wt%) of nanocellulose were investigated by oscillatory rheometer with parallel plate geometry.
24877644	3	22	theme	nanocellulose	415:427	arg1	amounts					386:392	various amounts	378:392	various amounts (0.5, 1 and 3wt%) of nanocellulose	378:427	Rheological properties of unsaturated polyester resin suspensions containing various amounts (0.5, 1 and 3wt%) of nanocellulose were investigated by oscillatory rheometer with parallel plate geometry.
24877644	6	23	theme	Tensile	806:812	arg1	properties					825:834	Tensile and impact properties	806:834	properties	825:834	Tensile and impact properties showed superior behaviour revealing improved interfacial bonding between nanofiller and the polymer matrix.
24877644	8	24	theme	polyester	1163:1171	arg1	matrix					1173:1178	the polyester matrix	1159:1178	the polyester matrix	1159:1178	Optical and atomic force microscopic studies confirmed that the dispersion state of NC within the polyester matrix was adequate.
24877644	8	25	theme	microscopic	1090:1100	arg1	studies					1102:1108	Optical and atomic force microscopic studies	1065:1108	Optical and atomic force microscopic studies	1065:1108	Optical and atomic force microscopic studies confirmed that the dispersion state of NC within the polyester matrix was adequate.
24877644	9	26	theme	10°C	1307:1310	arg1	increase					1295:1302	an increase	1292:1302	an increase of 10°C	1292:1310	Maximum glass transition temperature is obtained for 0.5wt% NC reinforced composite, which showed an increase of 10°C than neat resin.
24877644	5	27	theme	reinforced	672:681	arg1	suspensions					693:703	NC reinforced polyester suspensions	669:703	NC reinforced polyester suspensions	669:703	NC reinforced polyester suspensions showed shear thinning behaviour initially and at higher shear rates they showed Newtonian behaviour.
24877644	9	28	theme	transition	1208:1217	arg1	temperature					1219:1229	Maximum glass transition temperature	1194:1229	Maximum glass transition temperature	1194:1229	Maximum glass transition temperature is obtained for 0.5wt% NC reinforced composite, which showed an increase of 10°C than neat resin.
24877644	0	29	theme	Rheological	0:10	arg1	behaviour					12:20	Rheological behaviour	0:20	Rheological behaviour of nanocellulose	0:37	Rheological behaviour of nanocellulose reinforced unsaturated polyester nanocomposites.
24877644	4	30	theme	filled	572:577	arg1	UPR					579:581	NC filled UPR	569:581	NC filled UPR	569:581	Analysis of curing revealed that the time required for gelation in NC filled UPR is lower than neat resin, which describe the catalytic action of NC on cure reaction.
24877644	8	31	theme	force	1084:1088	arg1	studies					1102:1108	Optical and atomic force microscopic studies	1065:1108	Optical and atomic force microscopic studies	1065:1108	Optical and atomic force microscopic studies confirmed that the dispersion state of NC within the polyester matrix was adequate.
24877644	9	32	theme	0.5wt	1247:1251	arg1	NC					1254:1255	0.5wt% NC	1247:1255	0.5wt% NC	1247:1255	Maximum glass transition temperature is obtained for 0.5wt% NC reinforced composite, which showed an increase of 10°C than neat resin.
24877644	1	33	theme	reinforced	107:116	arg1	composites					146:155	Nanocellulose (NC) reinforced unsaturated polyester (UPR) composites	88:155	Nanocellulose (NC) reinforced unsaturated polyester (UPR) composites	88:155	Nanocellulose (NC) reinforced unsaturated polyester (UPR) composites were prepared by mechanical mixing process.
24877644	0	34	theme	nanocellulose	25:37	arg1	behaviour					12:20	Rheological behaviour	0:20	Rheological behaviour of nanocellulose	0:37	Rheological behaviour of nanocellulose reinforced unsaturated polyester nanocomposites.
24877644	4	35	theme	NC	569:570	arg1	UPR					579:581	NC filled UPR	569:581	NC filled UPR	569:581	Analysis of curing revealed that the time required for gelation in NC filled UPR is lower than neat resin, which describe the catalytic action of NC on cure reaction.
24877644	7	36	theme	neat	964:967	arg1	polyester					969:977	the neat polyester	960:977	the neat polyester	960:977	With respect to the neat polyester the percentage increase in tensile strength of 0.5wt% NC reinforced composite is 57%.
24877644	1	37	theme	unsaturated	118:128	arg1	UPR					141:143	UPR	141:143	UPR	141:143	Nanocellulose (NC) reinforced unsaturated polyester (UPR) composites were prepared by mechanical mixing process.
24877644	1	37	theme	unsaturated	118:128	arg1	polyester					130:138	unsaturated polyester	118:138	Nanocellulose (NC) reinforced unsaturated polyester (UPR) composites	88:155	Nanocellulose (NC) reinforced unsaturated polyester (UPR) composites were prepared by mechanical mixing process.
24877644	3	38	theme	plate	486:490	arg1	geometry					492:499	parallel plate geometry	477:499	parallel plate geometry	477:499	Rheological properties of unsaturated polyester resin suspensions containing various amounts (0.5, 1 and 3wt%) of nanocellulose were investigated by oscillatory rheometer with parallel plate geometry.
24877644	3	39	theme	Rheological	301:311	arg1	properties					313:322	Rheological properties	301:322	Rheological properties of unsaturated polyester resin suspensions containing various amounts (0.5, 1 and 3wt%) of nanocellulose	301:427	Rheological properties of unsaturated polyester resin suspensions containing various amounts (0.5, 1 and 3wt%) of nanocellulose were investigated by oscillatory rheometer with parallel plate geometry.
24877644	7	40	theme	reinforced	1036:1045	arg1	composite					1047:1055	0.5wt% NC reinforced composite	1026:1055	0.5wt% NC reinforced composite	1026:1055	With respect to the neat polyester the percentage increase in tensile strength of 0.5wt% NC reinforced composite is 57%.
24877644	1	41	theme	polyester	130:138	arg1	composites					146:155	Nanocellulose (NC) reinforced unsaturated polyester (UPR) composites	88:155	Nanocellulose (NC) reinforced unsaturated polyester (UPR) composites	88:155	Nanocellulose (NC) reinforced unsaturated polyester (UPR) composites were prepared by mechanical mixing process.
24877644	4	42	from	action	638:643	arg1	reaction					659:666	cure reaction	654:666	cure reaction	654:666	Analysis of curing revealed that the time required for gelation in NC filled UPR is lower than neat resin, which describe the catalytic action of NC on cure reaction.
24877644	4	43	theme	catalytic	628:636	arg1	action					638:643	the catalytic action	624:643	the catalytic action of NC on cure reaction	624:666	Analysis of curing revealed that the time required for gelation in NC filled UPR is lower than neat resin, which describe the catalytic action of NC on cure reaction.
24877644	0	44	theme	unsaturated	50:60	arg1	nanocomposites					72:85	unsaturated polyester nanocomposites	50:85	unsaturated polyester nanocomposites	50:85	Rheological behaviour of nanocellulose reinforced unsaturated polyester nanocomposites.
24877644	5	45	theme	thinning	718:725	arg1	behaviour					727:735	shear thinning behaviour	712:735	shear thinning behaviour	712:735	NC reinforced polyester suspensions showed shear thinning behaviour initially and at higher shear rates they showed Newtonian behaviour.
24877644	2	46	theme	polyester	252:260	arg1	composites					262:271	polyester composites	252:271	polyester composites	252:271	Effect of isora nanocellulose on the properties of polyester composites has been studied in detail.
24877644	4	47	theme	NC	648:649	arg1	action					638:643	the catalytic action	624:643	the catalytic action of NC on cure reaction	624:666	Analysis of curing revealed that the time required for gelation in NC filled UPR is lower than neat resin, which describe the catalytic action of NC on cure reaction.
24877644	3	48	theme	oscillatory	450:460	arg1	rheometer					462:470	oscillatory rheometer	450:470	oscillatory rheometer with parallel plate geometry	450:499	Rheological properties of unsaturated polyester resin suspensions containing various amounts (0.5, 1 and 3wt%) of nanocellulose were investigated by oscillatory rheometer with parallel plate geometry.
24877644	4	49	from	gelation	557:564	arg1	UPR					579:581	NC filled UPR	569:581	NC filled UPR	569:581	Analysis of curing revealed that the time required for gelation in NC filled UPR is lower than neat resin, which describe the catalytic action of NC on cure reaction.
24877644	7	50	theme	composite	1047:1055	arg1	strength					1014:1021	tensile strength	1006:1021	tensile strength of 0.5wt% NC reinforced composite	1006:1055	With respect to the neat polyester the percentage increase in tensile strength of 0.5wt% NC reinforced composite is 57%.
24877644	3	51	theme	parallel	477:484	arg1	geometry					492:499	parallel plate geometry	477:499	parallel plate geometry	477:499	Rheological properties of unsaturated polyester resin suspensions containing various amounts (0.5, 1 and 3wt%) of nanocellulose were investigated by oscillatory rheometer with parallel plate geometry.
24877644	3	52	theme	unsaturated	327:337	arg1	suspensions					355:365	unsaturated polyester resin suspensions	327:365	unsaturated polyester resin suspensions containing various amounts (0.5, 1 and 3wt%) of nanocellulose	327:427	Rheological properties of unsaturated polyester resin suspensions containing various amounts (0.5, 1 and 3wt%) of nanocellulose were investigated by oscillatory rheometer with parallel plate geometry.
24877644	6	53	theme	interfacial	881:891	arg1	bonding					893:899	improved interfacial bonding	872:899	improved interfacial bonding between nanofiller and the polymer matrix	872:941	Tensile and impact properties showed superior behaviour revealing improved interfacial bonding between nanofiller and the polymer matrix.
24877644	8	54	theme	dispersion	1129:1138	arg1	state					1140:1144	the dispersion state	1125:1144	the dispersion state of NC within the polyester matrix	1125:1178	Optical and atomic force microscopic studies confirmed that the dispersion state of NC within the polyester matrix was adequate.
24877644	8	54	theme	dispersion	1129:1138	arg1	adequate					1184:1191	adequate	1184:1191	adequate	1184:1191	Optical and atomic force microscopic studies confirmed that the dispersion state of NC within the polyester matrix was adequate.
24877644	0	55	theme	polyester	62:70	arg1	nanocomposites					72:85	unsaturated polyester nanocomposites	50:85	unsaturated polyester nanocomposites	50:85	Rheological behaviour of nanocellulose reinforced unsaturated polyester nanocomposites.
24877644	3	56	theme	polyester	339:347	arg1	suspensions					355:365	unsaturated polyester resin suspensions	327:365	unsaturated polyester resin suspensions containing various amounts (0.5, 1 and 3wt%) of nanocellulose	327:427	Rheological properties of unsaturated polyester resin suspensions containing various amounts (0.5, 1 and 3wt%) of nanocellulose were investigated by oscillatory rheometer with parallel plate geometry.
24877644	6	57	theme	improved	872:879	arg1	bonding					893:899	improved interfacial bonding	872:899	improved interfacial bonding between nanofiller and the polymer matrix	872:941	Tensile and impact properties showed superior behaviour revealing improved interfacial bonding between nanofiller and the polymer matrix.
24877644	7	58	theme	NC	1033:1034	arg1	composite					1047:1055	0.5wt% NC reinforced composite	1026:1055	0.5wt% NC reinforced composite	1026:1055	With respect to the neat polyester the percentage increase in tensile strength of 0.5wt% NC reinforced composite is 57%.
24877644	9	59	theme	%	1252:1252	arg1	NC					1254:1255	0.5wt% NC	1247:1255	0.5wt% NC	1247:1255	Maximum glass transition temperature is obtained for 0.5wt% NC reinforced composite, which showed an increase of 10°C than neat resin.
24877644	5	60	theme	polyester	683:691	arg1	suspensions					693:703	NC reinforced polyester suspensions	669:703	NC reinforced polyester suspensions	669:703	NC reinforced polyester suspensions showed shear thinning behaviour initially and at higher shear rates they showed Newtonian behaviour.
24877644	2	61	theme	nanocellulose	217:229	arg1	Effect					201:206	Effect	201:206	Effect of isora nanocellulose on the properties of polyester composites	201:271	Effect of isora nanocellulose on the properties of polyester composites has been studied in detail.
24877644	8	62	theme	NC	1149:1150	arg1	state					1140:1144	the dispersion state	1125:1144	the dispersion state of NC within the polyester matrix	1125:1178	Optical and atomic force microscopic studies confirmed that the dispersion state of NC within the polyester matrix was adequate.
24877644	8	62	theme	NC	1149:1150	arg1	adequate					1184:1191	adequate	1184:1191	adequate	1184:1191	Optical and atomic force microscopic studies confirmed that the dispersion state of NC within the polyester matrix was adequate.
24877644	7	63	theme	tensile	1006:1012	arg1	strength					1014:1021	tensile strength	1006:1021	tensile strength of 0.5wt% NC reinforced composite	1006:1055	With respect to the neat polyester the percentage increase in tensile strength of 0.5wt% NC reinforced composite is 57%.
24877644	3	64	with	rheometer	462:470	arg1	geometry					492:499	parallel plate geometry	477:499	parallel plate geometry	477:499	Rheological properties of unsaturated polyester resin suspensions containing various amounts (0.5, 1 and 3wt%) of nanocellulose were investigated by oscillatory rheometer with parallel plate geometry.
24877644	2	65	theme	isora	211:215	arg1	nanocellulose					217:229	isora nanocellulose	211:229	isora nanocellulose	211:229	Effect of isora nanocellulose on the properties of polyester composites has been studied in detail.
24877644	7	66	theme	0.5wt	1026:1030	arg1	composite					1047:1055	0.5wt% NC reinforced composite	1026:1055	0.5wt% NC reinforced composite	1026:1055	With respect to the neat polyester the percentage increase in tensile strength of 0.5wt% NC reinforced composite is 57%.
24877644	5	67	theme	shear	761:765	arg1	rates					767:771	higher shear rates	754:771	higher shear rates	754:771	NC reinforced polyester suspensions showed shear thinning behaviour initially and at higher shear rates they showed Newtonian behaviour.
24877644	6	68	theme	superior	843:850	arg1	behaviour					852:860	superior behaviour	843:860	superior behaviour	843:860	Tensile and impact properties showed superior behaviour revealing improved interfacial bonding between nanofiller and the polymer matrix.
24877644	7	69	theme	%	1031:1031	arg1	composite					1047:1055	0.5wt% NC reinforced composite	1026:1055	0.5wt% NC reinforced composite	1026:1055	With respect to the neat polyester the percentage increase in tensile strength of 0.5wt% NC reinforced composite is 57%.
24877644	3	70	theme	various	378:384	arg1	%					409:409	0.5, 1 and 3wt%	395:409	0.5, 1 and 3wt%	395:409	Rheological properties of unsaturated polyester resin suspensions containing various amounts (0.5, 1 and 3wt%) of nanocellulose were investigated by oscillatory rheometer with parallel plate geometry.
24877644	3	70	theme	various	378:384	arg1	nanocellulose					415:427	nanocellulose	415:427	nanocellulose	415:427	Rheological properties of unsaturated polyester resin suspensions containing various amounts (0.5, 1 and 3wt%) of nanocellulose were investigated by oscillatory rheometer with parallel plate geometry.
24877644	3	70	theme	various	378:384	arg1	amounts					386:392	various amounts	378:392	various amounts (0.5, 1 and 3wt%) of nanocellulose	378:427	Rheological properties of unsaturated polyester resin suspensions containing various amounts (0.5, 1 and 3wt%) of nanocellulose were investigated by oscillatory rheometer with parallel plate geometry.
24877644	2	71	theme	composites	262:271	arg1	properties					238:247	the properties	234:247	the properties of polyester composites	234:271	Effect of isora nanocellulose on the properties of polyester composites has been studied in detail.
24877644	7	72	from	increase	994:1001	arg1	strength					1014:1021	tensile strength	1006:1021	tensile strength of 0.5wt% NC reinforced composite	1006:1055	With respect to the neat polyester the percentage increase in tensile strength of 0.5wt% NC reinforced composite is 57%.
29269012	7	0	theme	300 mg/L	1083:1090	arg1	height					1042:1047	bed height	1038:1047	bed height of 3 cm, 2.0 mL/min flow rate and 300 mg/L	1038:1090	Maximum dynamic adsorption capacity was confirmed at bed height of 3 cm, 2.0 mL/min flow rate and 300 mg/L as Hg+2 concentration with breakthrough time (tb) and exhaustion time (te) of 9 and 21 h. Yoon-Nelson and Thomas models best described the experimental Hg+2 breakthrough curve model.
29269012	7	1	theme	3 cm	1052:1055	arg1	height					1042:1047	bed height	1038:1047	bed height of 3 cm, 2.0 mL/min flow rate and 300 mg/L	1038:1090	Maximum dynamic adsorption capacity was confirmed at bed height of 3 cm, 2.0 mL/min flow rate and 300 mg/L as Hg+2 concentration with breakthrough time (tb) and exhaustion time (te) of 9 and 21 h. Yoon-Nelson and Thomas models best described the experimental Hg+2 breakthrough curve model.
29269012	4	2	theme	time	621:624	arg1	effect					589:594	the effect	585:594	the effect of adsorbent dosage, pH, time and initial Hg+2 concentrations	585:656	Static adsorption of Hg+2 was tested for the effect of adsorbent dosage, pH, time and initial Hg+2 concentrations indicating that maximum static adsorption capacity was confirmed by CP12 (111.6 mg/g).
29269012	1	3	theme	chitosan	257:264	arg1	ratios					286:291	different chitosan: nanohydroxyapatite ratios	247:291	different chitosan: nanohydroxyapatite ratios (1:2, 1:4 and 2:1, respectively)	247:324	Chitosan/nanohydroxyapatite composites based on scallop shells (CP12, CP14 and CP21) were prepared with different chitosan: nanohydroxyapatite ratios (1:2, 1:4 and 2:1, respectively).
29269012	2	4	theme	adsorption/desorption	428:448	arg1	analysis					450:457	N2 adsorption/desorption analysis	425:457	N2 adsorption/desorption analysis	425:457	Nanohydroxyapatite (P), chitosan(C) and their composites were characterized by means of TGA, XRD, N2 adsorption/desorption analysis, SEM, Zeta potential and FTIR.
29269012	1	5	theme	scallop	191:197	arg1	CP12					207:210	CP12	207:210	CP12	207:210	Chitosan/nanohydroxyapatite composites based on scallop shells (CP12, CP14 and CP21) were prepared with different chitosan: nanohydroxyapatite ratios (1:2, 1:4 and 2:1, respectively).
29269012	1	5	theme	scallop	191:197	arg1	shells					199:204	scallop shells	191:204	scallop shells (CP12, CP14 and CP21)	191:226	Chitosan/nanohydroxyapatite composites based on scallop shells (CP12, CP14 and CP21) were prepared with different chitosan: nanohydroxyapatite ratios (1:2, 1:4 and 2:1, respectively).
29269012	1	5	theme	scallop	191:197	arg1	CP21					222:225	CP21	222:225	CP21	222:225	Chitosan/nanohydroxyapatite composites based on scallop shells (CP12, CP14 and CP21) were prepared with different chitosan: nanohydroxyapatite ratios (1:2, 1:4 and 2:1, respectively).
29269012	1	5	theme	scallop	191:197	arg1	CP14					213:216	CP14	213:216	CP14	213:216	Chitosan/nanohydroxyapatite composites based on scallop shells (CP12, CP14 and CP21) were prepared with different chitosan: nanohydroxyapatite ratios (1:2, 1:4 and 2:1, respectively).
29269012	8	6	theme	static	1281:1286	arg1	adsorption					1288:1297	static adsorption	1281:1297	static adsorption	1281:1297	After static adsorption, EDTA solution confirmed the maximum desorption efficiency.
29269012	7	7	theme	9	1170:1170	arg1	concentration					1100:1112	Hg+2 concentration	1095:1112	Hg+2 concentration with breakthrough time (tb) and exhaustion time (te) of 9 and 21 h. Yoon-Nelson and Thomas models	1095:1210	Maximum dynamic adsorption capacity was confirmed at bed height of 3 cm, 2.0 mL/min flow rate and 300 mg/L as Hg+2 concentration with breakthrough time (tb) and exhaustion time (te) of 9 and 21 h. Yoon-Nelson and Thomas models best described the experimental Hg+2 breakthrough curve model.
29269012	0	8	theme	Static	104:109	arg1	studies					134:140	Static and dynamic adsorption studies	104:140	Static and dynamic adsorption studies	104:140	Chitosan/nanohydroxyapatite composite based scallop shells as an efficient adsorbent for mercuric ions: Static and dynamic adsorption studies.
29269012	9	9	theme	adsorption-desorption	1430:1450	arg1	cycles					1405:1410	three cycles	1399:1410	three cycles of column dynamic adsorption-desorption	1399:1450	The validity of CP12 was tested through three cycles of column dynamic adsorption-desorption.
29269012	8	10	theme	maximum	1328:1334	arg1	efficiency					1347:1356	the maximum desorption efficiency	1324:1356	the maximum desorption efficiency	1324:1356	After static adsorption, EDTA solution confirmed the maximum desorption efficiency.
29269012	7	11	with	concentration	1100:1112	arg1	time					1132:1135	breakthrough time	1119:1135	breakthrough time (tb)	1119:1140	Maximum dynamic adsorption capacity was confirmed at bed height of 3 cm, 2.0 mL/min flow rate and 300 mg/L as Hg+2 concentration with breakthrough time (tb) and exhaustion time (te) of 9 and 21 h. Yoon-Nelson and Thomas models best described the experimental Hg+2 breakthrough curve model.
29269012	7	11	with	concentration	1100:1112	arg1	te					1163:1164	te	1163:1164	te	1163:1164	Maximum dynamic adsorption capacity was confirmed at bed height of 3 cm, 2.0 mL/min flow rate and 300 mg/L as Hg+2 concentration with breakthrough time (tb) and exhaustion time (te) of 9 and 21 h. Yoon-Nelson and Thomas models best described the experimental Hg+2 breakthrough curve model.
29269012	7	11	with	concentration	1100:1112	arg1	time					1157:1160	exhaustion time	1146:1160	exhaustion time (te)	1146:1165	Maximum dynamic adsorption capacity was confirmed at bed height of 3 cm, 2.0 mL/min flow rate and 300 mg/L as Hg+2 concentration with breakthrough time (tb) and exhaustion time (te) of 9 and 21 h. Yoon-Nelson and Thomas models best described the experimental Hg+2 breakthrough curve model.
29269012	7	11	with	concentration	1100:1112	arg1	tb					1138:1139	tb	1138:1139	tb	1138:1139	Maximum dynamic adsorption capacity was confirmed at bed height of 3 cm, 2.0 mL/min flow rate and 300 mg/L as Hg+2 concentration with breakthrough time (tb) and exhaustion time (te) of 9 and 21 h. Yoon-Nelson and Thomas models best described the experimental Hg+2 breakthrough curve model.
29269012	1	12	theme	nanohydroxyapatite	267:284	arg1	ratios					286:291	different chitosan: nanohydroxyapatite ratios	247:291	different chitosan: nanohydroxyapatite ratios (1:2, 1:4 and 2:1, respectively)	247:324	Chitosan/nanohydroxyapatite composites based on scallop shells (CP12, CP14 and CP21) were prepared with different chitosan: nanohydroxyapatite ratios (1:2, 1:4 and 2:1, respectively).
29269012	9	13	theme	column	1415:1420	arg1	adsorption-desorption					1430:1450	column dynamic adsorption-desorption	1415:1450	column dynamic adsorption-desorption	1415:1450	The validity of CP12 was tested through three cycles of column dynamic adsorption-desorption.
29269012	4	14	theme	adsorption	689:698	arg1	capacity					700:707	maximum static adsorption capacity	674:707	maximum static adsorption capacity	674:707	Static adsorption of Hg+2 was tested for the effect of adsorbent dosage, pH, time and initial Hg+2 concentrations indicating that maximum static adsorption capacity was confirmed by CP12 (111.6 mg/g).
29269012	7	15	theme	rate	1074:1077	arg1	height					1042:1047	bed height	1038:1047	bed height of 3 cm, 2.0 mL/min flow rate and 300 mg/L	1038:1090	Maximum dynamic adsorption capacity was confirmed at bed height of 3 cm, 2.0 mL/min flow rate and 300 mg/L as Hg+2 concentration with breakthrough time (tb) and exhaustion time (te) of 9 and 21 h. Yoon-Nelson and Thomas models best described the experimental Hg+2 breakthrough curve model.
29269012	3	16	theme	BET	494:496	arg1	area					506:509	The BET surface area	490:509	The BET surface area	490:509	The BET surface area ranged between 189 and 512 m2/g.
29269012	4	17	theme	Hg+2	638:641	arg1	concentrations					643:656	initial Hg+2 concentrations	630:656	initial Hg+2 concentrations	630:656	Static adsorption of Hg+2 was tested for the effect of adsorbent dosage, pH, time and initial Hg+2 concentrations indicating that maximum static adsorption capacity was confirmed by CP12 (111.6 mg/g).
29269012	2	18	theme	Zeta	465:468	arg1	potential					470:478	Zeta potential	465:478	Zeta potential	465:478	Nanohydroxyapatite (P), chitosan(C) and their composites were characterized by means of TGA, XRD, N2 adsorption/desorption analysis, SEM, Zeta potential and FTIR.
29269012	6	19	theme	concentrations	969:982	arg1	effect					914:919	the effect	910:919	the effect of bed height	910:933	CP12 was selected for dynamic adsorption of Hg+2 considering the effect of bed height, flow rate and the effect of Hg+2 concentrations.
29269012	6	19	theme	concentrations	969:982	arg1	rate					941:944	flow rate	936:944	flow rate	936:944	CP12 was selected for dynamic adsorption of Hg+2 considering the effect of bed height, flow rate and the effect of Hg+2 concentrations.
29269012	6	19	theme	concentrations	969:982	arg1	effect					954:959	the effect	950:959	the effect of Hg+2 concentrations	950:982	CP12 was selected for dynamic adsorption of Hg+2 considering the effect of bed height, flow rate and the effect of Hg+2 concentrations.
29269012	5	20	theme	adsorption	789:798	arg1	isotherm					800:807	Langmuir adsorption isotherm	780:807	Langmuir adsorption isotherm	780:807	Static adsorption well fitted with Langmuir adsorption isotherm and Pseudo-second order kinetic models.
29269012	6	21	theme	bed	924:926	arg1	height					928:933	bed height	924:933	bed height	924:933	CP12 was selected for dynamic adsorption of Hg+2 considering the effect of bed height, flow rate and the effect of Hg+2 concentrations.
29269012	6	22	theme	flow	936:939	arg1	rate					941:944	flow rate	936:944	flow rate	936:944	CP12 was selected for dynamic adsorption of Hg+2 considering the effect of bed height, flow rate and the effect of Hg+2 concentrations.
29269012	7	23	dep	9	1170:1170	arg1	models					1205:1210	models	1205:1210	models	1205:1210	Maximum dynamic adsorption capacity was confirmed at bed height of 3 cm, 2.0 mL/min flow rate and 300 mg/L as Hg+2 concentration with breakthrough time (tb) and exhaustion time (te) of 9 and 21 h. Yoon-Nelson and Thomas models best described the experimental Hg+2 breakthrough curve model.
29269012	0	24	theme	dynamic	115:121	arg1	studies					134:140	Static and dynamic adsorption studies	104:140	Static and dynamic adsorption studies	104:140	Chitosan/nanohydroxyapatite composite based scallop shells as an efficient adsorbent for mercuric ions: Static and dynamic adsorption studies.
29269012	0	25	theme	composite	28:36	arg1	adsorbent					75:83	adsorbent	75:83	adsorbent	75:83	Chitosan/nanohydroxyapatite composite based scallop shells as an efficient adsorbent for mercuric ions: Static and dynamic adsorption studies.
29269012	0	25	theme	composite	28:36	arg1	shells					52:57	composite based scallop shells	28:57	composite based scallop shells	28:57	Chitosan/nanohydroxyapatite composite based scallop shells as an efficient adsorbent for mercuric ions: Static and dynamic adsorption studies.
29269012	0	26	theme	mercuric	89:96	arg1	ions					98:101	mercuric ions	89:101	mercuric ions	89:101	Chitosan/nanohydroxyapatite composite based scallop shells as an efficient adsorbent for mercuric ions: Static and dynamic adsorption studies.
29269012	7	27	theme	Hg+2	1095:1098	arg1	concentration					1100:1112	Hg+2 concentration	1095:1112	Hg+2 concentration with breakthrough time (tb) and exhaustion time (te) of 9 and 21 h. Yoon-Nelson and Thomas models	1095:1210	Maximum dynamic adsorption capacity was confirmed at bed height of 3 cm, 2.0 mL/min flow rate and 300 mg/L as Hg+2 concentration with breakthrough time (tb) and exhaustion time (te) of 9 and 21 h. Yoon-Nelson and Thomas models best described the experimental Hg+2 breakthrough curve model.
29269012	4	28	theme	dosage	609:614	arg1	effect					589:594	the effect	585:594	the effect of adsorbent dosage, pH, time and initial Hg+2 concentrations	585:656	Static adsorption of Hg+2 was tested for the effect of adsorbent dosage, pH, time and initial Hg+2 concentrations indicating that maximum static adsorption capacity was confirmed by CP12 (111.6 mg/g).
29269012	4	29	theme	adsorbent	599:607	arg1	dosage					609:614	adsorbent dosage	599:614	adsorbent dosage	599:614	Static adsorption of Hg+2 was tested for the effect of adsorbent dosage, pH, time and initial Hg+2 concentrations indicating that maximum static adsorption capacity was confirmed by CP12 (111.6 mg/g).
29269012	0	30	theme	scallop	44:50	arg1	adsorbent					75:83	adsorbent	75:83	adsorbent	75:83	Chitosan/nanohydroxyapatite composite based scallop shells as an efficient adsorbent for mercuric ions: Static and dynamic adsorption studies.
29269012	0	30	theme	scallop	44:50	arg1	shells					52:57	composite based scallop shells	28:57	composite based scallop shells	28:57	Chitosan/nanohydroxyapatite composite based scallop shells as an efficient adsorbent for mercuric ions: Static and dynamic adsorption studies.
29269012	7	31	theme	adsorption	1001:1010	arg1	capacity					1012:1019	Maximum dynamic adsorption capacity	985:1019	Maximum dynamic adsorption capacity	985:1019	Maximum dynamic adsorption capacity was confirmed at bed height of 3 cm, 2.0 mL/min flow rate and 300 mg/L as Hg+2 concentration with breakthrough time (tb) and exhaustion time (te) of 9 and 21 h. Yoon-Nelson and Thomas models best described the experimental Hg+2 breakthrough curve model.
29269012	7	32	theme	breakthrough	1249:1260	arg1	model					1268:1272	the experimental Hg+2 breakthrough curve model	1227:1272	the experimental Hg+2 breakthrough curve model	1227:1272	Maximum dynamic adsorption capacity was confirmed at bed height of 3 cm, 2.0 mL/min flow rate and 300 mg/L as Hg+2 concentration with breakthrough time (tb) and exhaustion time (te) of 9 and 21 h. Yoon-Nelson and Thomas models best described the experimental Hg+2 breakthrough curve model.
29269012	3	33	theme	surface	498:504	arg1	area					506:509	The BET surface area	490:509	The BET surface area	490:509	The BET surface area ranged between 189 and 512 m2/g.
29269012	9	34	theme	CP12	1375:1378	arg1	validity					1363:1370	The validity	1359:1370	The validity of CP12	1359:1378	The validity of CP12 was tested through three cycles of column dynamic adsorption-desorption.
29269012	0	35	theme	based	38:42	arg1	adsorbent					75:83	adsorbent	75:83	adsorbent	75:83	Chitosan/nanohydroxyapatite composite based scallop shells as an efficient adsorbent for mercuric ions: Static and dynamic adsorption studies.
29269012	0	35	theme	based	38:42	arg1	shells					52:57	composite based scallop shells	28:57	composite based scallop shells	28:57	Chitosan/nanohydroxyapatite composite based scallop shells as an efficient adsorbent for mercuric ions: Static and dynamic adsorption studies.
29269012	0	36	theme	adsorption	123:132	arg1	studies					134:140	Static and dynamic adsorption studies	104:140	Static and dynamic adsorption studies	104:140	Chitosan/nanohydroxyapatite composite based scallop shells as an efficient adsorbent for mercuric ions: Static and dynamic adsorption studies.
29269012	4	37	theme	maximum	674:680	arg1	capacity					700:707	maximum static adsorption capacity	674:707	maximum static adsorption capacity	674:707	Static adsorption of Hg+2 was tested for the effect of adsorbent dosage, pH, time and initial Hg+2 concentrations indicating that maximum static adsorption capacity was confirmed by CP12 (111.6 mg/g).
29269012	5	38	theme	order	827:831	arg1	models					841:846	Pseudo-second order kinetic models	813:846	Pseudo-second order kinetic models	813:846	Static adsorption well fitted with Langmuir adsorption isotherm and Pseudo-second order kinetic models.
29269012	2	39	theme	N2	425:426	arg1	analysis					450:457	N2 adsorption/desorption analysis	425:457	N2 adsorption/desorption analysis	425:457	Nanohydroxyapatite (P), chitosan(C) and their composites were characterized by means of TGA, XRD, N2 adsorption/desorption analysis, SEM, Zeta potential and FTIR.
29269012	7	40	theme	Maximum	985:991	arg1	capacity					1012:1019	Maximum dynamic adsorption capacity	985:1019	Maximum dynamic adsorption capacity	985:1019	Maximum dynamic adsorption capacity was confirmed at bed height of 3 cm, 2.0 mL/min flow rate and 300 mg/L as Hg+2 concentration with breakthrough time (tb) and exhaustion time (te) of 9 and 21 h. Yoon-Nelson and Thomas models best described the experimental Hg+2 breakthrough curve model.
29269012	7	41	theme	Yoon-Nelson	1182:1192	arg1	concentration					1100:1112	Hg+2 concentration	1095:1112	Hg+2 concentration with breakthrough time (tb) and exhaustion time (te) of 9 and 21 h. Yoon-Nelson and Thomas models	1095:1210	Maximum dynamic adsorption capacity was confirmed at bed height of 3 cm, 2.0 mL/min flow rate and 300 mg/L as Hg+2 concentration with breakthrough time (tb) and exhaustion time (te) of 9 and 21 h. Yoon-Nelson and Thomas models best described the experimental Hg+2 breakthrough curve model.
29269012	8	42	theme	EDTA	1300:1303	arg1	solution					1305:1312	EDTA solution	1300:1312	EDTA solution	1300:1312	After static adsorption, EDTA solution confirmed the maximum desorption efficiency.
29269012	5	43	theme	Pseudo-second	813:825	arg1	models					841:846	Pseudo-second order kinetic models	813:846	Pseudo-second order kinetic models	813:846	Static adsorption well fitted with Langmuir adsorption isotherm and Pseudo-second order kinetic models.
29269012	6	44	theme	Hg+2	964:967	arg1	concentrations					969:982	Hg+2 concentrations	964:982	Hg+2 concentrations	964:982	CP12 was selected for dynamic adsorption of Hg+2 considering the effect of bed height, flow rate and the effect of Hg+2 concentrations.
29269012	6	45	theme	Hg+2	893:896	arg1	adsorption					879:888	dynamic adsorption	871:888	dynamic adsorption of Hg+2 considering the effect of bed height, flow rate and the effect of Hg+2 concentrations	871:982	CP12 was selected for dynamic adsorption of Hg+2 considering the effect of bed height, flow rate and the effect of Hg+2 concentrations.
29269012	7	46	theme	dynamic	993:999	arg1	capacity					1012:1019	Maximum dynamic adsorption capacity	985:1019	Maximum dynamic adsorption capacity	985:1019	Maximum dynamic adsorption capacity was confirmed at bed height of 3 cm, 2.0 mL/min flow rate and 300 mg/L as Hg+2 concentration with breakthrough time (tb) and exhaustion time (te) of 9 and 21 h. Yoon-Nelson and Thomas models best described the experimental Hg+2 breakthrough curve model.
29269012	5	47	theme	Static	745:750	arg1	adsorption					752:761	Static adsorption	745:761	Static adsorption	745:761	Static adsorption well fitted with Langmuir adsorption isotherm and Pseudo-second order kinetic models.
29269012	5	48	theme	Langmuir	780:787	arg1	adsorption					789:798	Langmuir adsorption	780:798	Langmuir adsorption isotherm	780:807	Static adsorption well fitted with Langmuir adsorption isotherm and Pseudo-second order kinetic models.
29269012	7	49	theme	2.0 mL/min	1058:1067	arg1	rate					1074:1077	2.0 mL/min flow rate	1058:1077	2.0 mL/min flow rate	1058:1077	Maximum dynamic adsorption capacity was confirmed at bed height of 3 cm, 2.0 mL/min flow rate and 300 mg/L as Hg+2 concentration with breakthrough time (tb) and exhaustion time (te) of 9 and 21 h. Yoon-Nelson and Thomas models best described the experimental Hg+2 breakthrough curve model.
29269012	7	50	theme	exhaustion	1146:1155	arg1	te					1163:1164	te	1163:1164	te	1163:1164	Maximum dynamic adsorption capacity was confirmed at bed height of 3 cm, 2.0 mL/min flow rate and 300 mg/L as Hg+2 concentration with breakthrough time (tb) and exhaustion time (te) of 9 and 21 h. Yoon-Nelson and Thomas models best described the experimental Hg+2 breakthrough curve model.
29269012	7	50	theme	exhaustion	1146:1155	arg1	time					1157:1160	exhaustion time	1146:1160	exhaustion time (te)	1146:1165	Maximum dynamic adsorption capacity was confirmed at bed height of 3 cm, 2.0 mL/min flow rate and 300 mg/L as Hg+2 concentration with breakthrough time (tb) and exhaustion time (te) of 9 and 21 h. Yoon-Nelson and Thomas models best described the experimental Hg+2 breakthrough curve model.
29269012	1	51	dep	shells	199:204	arg1	CP12					207:210	CP12	207:210	CP12	207:210	Chitosan/nanohydroxyapatite composites based on scallop shells (CP12, CP14 and CP21) were prepared with different chitosan: nanohydroxyapatite ratios (1:2, 1:4 and 2:1, respectively).
29269012	1	51	dep	shells	199:204	arg1	shells					199:204	scallop shells	191:204	scallop shells (CP12, CP14 and CP21)	191:226	Chitosan/nanohydroxyapatite composites based on scallop shells (CP12, CP14 and CP21) were prepared with different chitosan: nanohydroxyapatite ratios (1:2, 1:4 and 2:1, respectively).
29269012	1	51	dep	shells	199:204	arg1	CP21					222:225	CP21	222:225	CP21	222:225	Chitosan/nanohydroxyapatite composites based on scallop shells (CP12, CP14 and CP21) were prepared with different chitosan: nanohydroxyapatite ratios (1:2, 1:4 and 2:1, respectively).
29269012	1	51	dep	shells	199:204	arg1	CP14					213:216	CP14	213:216	CP14	213:216	Chitosan/nanohydroxyapatite composites based on scallop shells (CP12, CP14 and CP21) were prepared with different chitosan: nanohydroxyapatite ratios (1:2, 1:4 and 2:1, respectively).
29269012	4	52	theme	initial	630:636	arg1	concentrations					643:656	initial Hg+2 concentrations	630:656	initial Hg+2 concentrations	630:656	Static adsorption of Hg+2 was tested for the effect of adsorbent dosage, pH, time and initial Hg+2 concentrations indicating that maximum static adsorption capacity was confirmed by CP12 (111.6 mg/g).
29269012	7	53	theme	flow	1069:1072	arg1	rate					1074:1077	2.0 mL/min flow rate	1058:1077	2.0 mL/min flow rate	1058:1077	Maximum dynamic adsorption capacity was confirmed at bed height of 3 cm, 2.0 mL/min flow rate and 300 mg/L as Hg+2 concentration with breakthrough time (tb) and exhaustion time (te) of 9 and 21 h. Yoon-Nelson and Thomas models best described the experimental Hg+2 breakthrough curve model.
29269012	7	54	theme	bed	1038:1040	arg1	height					1042:1047	bed height	1038:1047	bed height of 3 cm, 2.0 mL/min flow rate and 300 mg/L	1038:1090	Maximum dynamic adsorption capacity was confirmed at bed height of 3 cm, 2.0 mL/min flow rate and 300 mg/L as Hg+2 concentration with breakthrough time (tb) and exhaustion time (te) of 9 and 21 h. Yoon-Nelson and Thomas models best described the experimental Hg+2 breakthrough curve model.
29269012	6	55	theme	dynamic	871:877	arg1	adsorption					879:888	dynamic adsorption	871:888	dynamic adsorption of Hg+2 considering the effect of bed height, flow rate and the effect of Hg+2 concentrations	871:982	CP12 was selected for dynamic adsorption of Hg+2 considering the effect of bed height, flow rate and the effect of Hg+2 concentrations.
29269012	1	56	dep	ratios	286:291	arg1	2:1					307:309	2:1	307:309	2:1	307:309	Chitosan/nanohydroxyapatite composites based on scallop shells (CP12, CP14 and CP21) were prepared with different chitosan: nanohydroxyapatite ratios (1:2, 1:4 and 2:1, respectively).
29269012	1	56	dep	ratios	286:291	arg1	1:4					299:301	1:4	299:301	1:4	299:301	Chitosan/nanohydroxyapatite composites based on scallop shells (CP12, CP14 and CP21) were prepared with different chitosan: nanohydroxyapatite ratios (1:2, 1:4 and 2:1, respectively).
29269012	1	56	dep	ratios	286:291	arg1	1:2					294:296	1:2	294:296	1:2	294:296	Chitosan/nanohydroxyapatite composites based on scallop shells (CP12, CP14 and CP21) were prepared with different chitosan: nanohydroxyapatite ratios (1:2, 1:4 and 2:1, respectively).
29269012	7	57	theme	curve	1262:1266	arg1	model					1268:1272	the experimental Hg+2 breakthrough curve model	1227:1272	the experimental Hg+2 breakthrough curve model	1227:1272	Maximum dynamic adsorption capacity was confirmed at bed height of 3 cm, 2.0 mL/min flow rate and 300 mg/L as Hg+2 concentration with breakthrough time (tb) and exhaustion time (te) of 9 and 21 h. Yoon-Nelson and Thomas models best described the experimental Hg+2 breakthrough curve model.
29269012	4	58	theme	concentrations	643:656	arg1	effect					589:594	the effect	585:594	the effect of adsorbent dosage, pH, time and initial Hg+2 concentrations	585:656	Static adsorption of Hg+2 was tested for the effect of adsorbent dosage, pH, time and initial Hg+2 concentrations indicating that maximum static adsorption capacity was confirmed by CP12 (111.6 mg/g).
29269012	4	59	theme	static	682:687	arg1	capacity					700:707	maximum static adsorption capacity	674:707	maximum static adsorption capacity	674:707	Static adsorption of Hg+2 was tested for the effect of adsorbent dosage, pH, time and initial Hg+2 concentrations indicating that maximum static adsorption capacity was confirmed by CP12 (111.6 mg/g).
29269012	8	60	theme	desorption	1336:1345	arg1	efficiency					1347:1356	the maximum desorption efficiency	1324:1356	the maximum desorption efficiency	1324:1356	After static adsorption, EDTA solution confirmed the maximum desorption efficiency.
29269012	1	61	theme	Chitosan/nanohydroxyapatite	143:169	arg1	composites					171:180	Chitosan/nanohydroxyapatite composites	143:180	Chitosan/nanohydroxyapatite composites based on scallop shells (CP12, CP14 and CP21)	143:226	Chitosan/nanohydroxyapatite composites based on scallop shells (CP12, CP14 and CP21) were prepared with different chitosan: nanohydroxyapatite ratios (1:2, 1:4 and 2:1, respectively).
29269012	4	62	theme	Static	544:549	arg1	adsorption					551:560	Static adsorption	544:560	Static adsorption of Hg+2	544:568	Static adsorption of Hg+2 was tested for the effect of adsorbent dosage, pH, time and initial Hg+2 concentrations indicating that maximum static adsorption capacity was confirmed by CP12 (111.6 mg/g).
29269012	7	63	theme	Hg+2	1244:1247	arg1	model					1268:1272	the experimental Hg+2 breakthrough curve model	1227:1272	the experimental Hg+2 breakthrough curve model	1227:1272	Maximum dynamic adsorption capacity was confirmed at bed height of 3 cm, 2.0 mL/min flow rate and 300 mg/L as Hg+2 concentration with breakthrough time (tb) and exhaustion time (te) of 9 and 21 h. Yoon-Nelson and Thomas models best described the experimental Hg+2 breakthrough curve model.
29269012	9	64	theme	dynamic	1422:1428	arg1	adsorption-desorption					1430:1450	column dynamic adsorption-desorption	1415:1450	column dynamic adsorption-desorption	1415:1450	The validity of CP12 was tested through three cycles of column dynamic adsorption-desorption.
29269012	4	65	theme	pH	617:618	arg1	effect					589:594	the effect	585:594	the effect of adsorbent dosage, pH, time and initial Hg+2 concentrations	585:656	Static adsorption of Hg+2 was tested for the effect of adsorbent dosage, pH, time and initial Hg+2 concentrations indicating that maximum static adsorption capacity was confirmed by CP12 (111.6 mg/g).
29269012	7	66	theme	experimental	1231:1242	arg1	model					1268:1272	the experimental Hg+2 breakthrough curve model	1227:1272	the experimental Hg+2 breakthrough curve model	1227:1272	Maximum dynamic adsorption capacity was confirmed at bed height of 3 cm, 2.0 mL/min flow rate and 300 mg/L as Hg+2 concentration with breakthrough time (tb) and exhaustion time (te) of 9 and 21 h. Yoon-Nelson and Thomas models best described the experimental Hg+2 breakthrough curve model.
29269012	4	67	theme	Hg+2	565:568	arg1	adsorption					551:560	Static adsorption	544:560	Static adsorption of Hg+2	544:568	Static adsorption of Hg+2 was tested for the effect of adsorbent dosage, pH, time and initial Hg+2 concentrations indicating that maximum static adsorption capacity was confirmed by CP12 (111.6 mg/g).
29269012	6	68	theme	height	928:933	arg1	effect					914:919	the effect	910:919	the effect of bed height	910:933	CP12 was selected for dynamic adsorption of Hg+2 considering the effect of bed height, flow rate and the effect of Hg+2 concentrations.
29269012	6	68	theme	height	928:933	arg1	rate					941:944	flow rate	936:944	flow rate	936:944	CP12 was selected for dynamic adsorption of Hg+2 considering the effect of bed height, flow rate and the effect of Hg+2 concentrations.
29269012	6	68	theme	height	928:933	arg1	effect					954:959	the effect	950:959	the effect of Hg+2 concentrations	950:982	CP12 was selected for dynamic adsorption of Hg+2 considering the effect of bed height, flow rate and the effect of Hg+2 concentrations.
29269012	0	69	dep	Chitosan/nanohydroxyapatite	0:26	arg1	studies					134:140	Static and dynamic adsorption studies	104:140	Static and dynamic adsorption studies	104:140	Chitosan/nanohydroxyapatite composite based scallop shells as an efficient adsorbent for mercuric ions: Static and dynamic adsorption studies.
29269012	5	70	theme	kinetic	833:839	arg1	models					841:846	Pseudo-second order kinetic models	813:846	Pseudo-second order kinetic models	813:846	Static adsorption well fitted with Langmuir adsorption isotherm and Pseudo-second order kinetic models.
29269012	1	71	theme	different	247:255	arg1	ratios					286:291	different chitosan: nanohydroxyapatite ratios	247:291	different chitosan: nanohydroxyapatite ratios (1:2, 1:4 and 2:1, respectively)	247:324	Chitosan/nanohydroxyapatite composites based on scallop shells (CP12, CP14 and CP21) were prepared with different chitosan: nanohydroxyapatite ratios (1:2, 1:4 and 2:1, respectively).
26480962	0	0	theme	in	89:90	arg1	studies					97:103	in vivo studies	89:103	Development of chitosan-pullulan composite nanoparticles for nasal delivery of vaccines: in vivo studies.	0:104	Development of chitosan-pullulan composite nanoparticles for nasal delivery of vaccines: in vivo studies.
26480962	6	1	theme	CpG	929:931	arg1	ODN					933:935	CpG ODN	929:935	CpG ODN	929:935	Co-encapsulation of CpG ODN within TMC-CMP-DT nanoparticles resulted in a balanced Th1/Th2 response.
26480962	1	2	theme	composite	153:161	arg1	nanoparticles					163:175	chitosan/pullulan composite nanoparticles	135:175	chitosan/pullulan composite nanoparticles	135:175	Here, we aimed at developing chitosan/pullulan composite nanoparticles and testing their potential as novel systems for the nasal delivery of diphtheria toxoid (DT).
26480962	1	3	theme	diphtheria	248:257	arg1	toxoid					259:264	diphtheria toxoid	248:264	diphtheria toxoid (DT)	248:269	Here, we aimed at developing chitosan/pullulan composite nanoparticles and testing their potential as novel systems for the nasal delivery of diphtheria toxoid (DT).
26480962	1	3	theme	diphtheria	248:257	arg1	DT					267:268	DT	267:268	DT	267:268	Here, we aimed at developing chitosan/pullulan composite nanoparticles and testing their potential as novel systems for the nasal delivery of diphtheria toxoid (DT).
26480962	2	4	theme	chitosan	466:473	arg1	complexation					450:461	polyion complexation	442:461	polyion complexation of chitosan and pullulan derivatives (particle size: 239-405 nm; surface charge: +18 and +27 mV)	442:558	All the chitosan derivatives [N-trimethyl (TMC), chloride and glutamate] and carboxymethyl pullulan (CMP) were synthesised and antigen-loaded composites were prepared by polyion complexation of chitosan and pullulan derivatives (particle size: 239-405 nm; surface charge: +18 and +27 mV).
26480962	4	5	theme	Composite	667:675	arg1	nanoparticles					677:689	Composite nanoparticles	667:689	Composite nanoparticles	667:689	Composite nanoparticles induced higher levels of IgG responses than particles formed with chitosan derivative and antigen.
26480962	1	6	theme	toxoid	259:264	arg1	delivery					236:243	the nasal delivery	226:243	the nasal delivery of diphtheria toxoid (DT)	226:269	Here, we aimed at developing chitosan/pullulan composite nanoparticles and testing their potential as novel systems for the nasal delivery of diphtheria toxoid (DT).
26480962	8	7	theme	promising	1193:1201	arg1	system					1212:1217	promising delivery system	1193:1217	promising delivery system for nasal vaccination	1193:1239	These findings demonstrated that TMC-CMP-DT composite nanoparticles are promising delivery system for nasal vaccination.
26480962	8	7	theme	promising	1193:1201	arg1	nanoparticles					1175:1187	TMC-CMP-DT composite nanoparticles	1154:1187	TMC-CMP-DT composite nanoparticles	1154:1187	These findings demonstrated that TMC-CMP-DT composite nanoparticles are promising delivery system for nasal vaccination.
26480962	4	8	theme	responses	720:728	arg1	levels					706:711	higher levels	699:711	higher levels of IgG responses	699:728	Composite nanoparticles induced higher levels of IgG responses than particles formed with chitosan derivative and antigen.
26480962	2	9	theme	particle	501:508	arg1	derivatives					488:498	pullulan derivatives	479:498	pullulan derivatives (particle size: 239-405 nm; surface charge: +18 and +27 mV)	479:558	All the chitosan derivatives [N-trimethyl (TMC), chloride and glutamate] and carboxymethyl pullulan (CMP) were synthesised and antigen-loaded composites were prepared by polyion complexation of chitosan and pullulan derivatives (particle size: 239-405 nm; surface charge: +18 and +27 mV).
26480962	2	9	theme	particle	501:508	arg1	size					510:513	particle size	501:513	particle size: 239-405 nm; surface charge	501:541	All the chitosan derivatives [N-trimethyl (TMC), chloride and glutamate] and carboxymethyl pullulan (CMP) were synthesised and antigen-loaded composites were prepared by polyion complexation of chitosan and pullulan derivatives (particle size: 239-405 nm; surface charge: +18 and +27 mV).
26480962	4	10	theme	higher	699:704	arg1	levels					706:711	higher levels	699:711	higher levels of IgG responses	699:728	Composite nanoparticles induced higher levels of IgG responses than particles formed with chitosan derivative and antigen.
26480962	5	11	theme	DT	849:850	arg1	titre					862:866	higher DT serum IgG titre	842:866	higher DT serum IgG titre	842:866	Nasally administered TMC-pullulan composites showed higher DT serum IgG titre when compared with the other composites.
26480962	4	12	theme	chitosan	757:764	arg1	derivative					766:775	chitosan derivative	757:775	chitosan derivative	757:775	Composite nanoparticles induced higher levels of IgG responses than particles formed with chitosan derivative and antigen.
26480962	5	13	theme	administered	798:809	arg1	composites					824:833	Nasally administered TMC-pullulan composites	790:833	Nasally administered TMC-pullulan composites	790:833	Nasally administered TMC-pullulan composites showed higher DT serum IgG titre when compared with the other composites.
26480962	8	14	theme	delivery	1203:1210	arg1	system					1212:1217	promising delivery system	1193:1217	promising delivery system for nasal vaccination	1193:1239	These findings demonstrated that TMC-CMP-DT composite nanoparticles are promising delivery system for nasal vaccination.
26480962	8	14	theme	delivery	1203:1210	arg1	nanoparticles					1175:1187	TMC-CMP-DT composite nanoparticles	1154:1187	TMC-CMP-DT composite nanoparticles	1154:1187	These findings demonstrated that TMC-CMP-DT composite nanoparticles are promising delivery system for nasal vaccination.
26480962	2	15	theme	derivatives	488:498	arg1	complexation					450:461	polyion complexation	442:461	polyion complexation of chitosan and pullulan derivatives (particle size: 239-405 nm; surface charge: +18 and +27 mV)	442:558	All the chitosan derivatives [N-trimethyl (TMC), chloride and glutamate] and carboxymethyl pullulan (CMP) were synthesised and antigen-loaded composites were prepared by polyion complexation of chitosan and pullulan derivatives (particle size: 239-405 nm; surface charge: +18 and +27 mV).
26480962	0	16	dep	in	89:90	arg1	vivo					92:95	vivo	92:95	vivo	92:95	Development of chitosan-pullulan composite nanoparticles for nasal delivery of vaccines: in vivo studies.
26480962	5	17	theme	TMC-pullulan	811:822	arg1	composites					824:833	Nasally administered TMC-pullulan composites	790:833	Nasally administered TMC-pullulan composites	790:833	Nasally administered TMC-pullulan composites showed higher DT serum IgG titre when compared with the other composites.
26480962	6	18	theme	Th1/Th2	992:998	arg1	response					1000:1007	a balanced Th1/Th2 response	981:1007	a balanced Th1/Th2 response	981:1007	Co-encapsulation of CpG ODN within TMC-CMP-DT nanoparticles resulted in a balanced Th1/Th2 response.
26480962	4	19	theme	IgG	716:718	arg1	responses					720:728	IgG responses	716:728	IgG responses	716:728	Composite nanoparticles induced higher levels of IgG responses than particles formed with chitosan derivative and antigen.
26480962	2	20	theme	carboxymethyl	349:361	arg1	CMP					373:375	CMP	373:375	CMP	373:375	All the chitosan derivatives [N-trimethyl (TMC), chloride and glutamate] and carboxymethyl pullulan (CMP) were synthesised and antigen-loaded composites were prepared by polyion complexation of chitosan and pullulan derivatives (particle size: 239-405 nm; surface charge: +18 and +27 mV).
26480962	2	20	theme	carboxymethyl	349:361	arg1	pullulan					363:370	carboxymethyl pullulan	349:370	carboxymethyl pullulan (CMP)	349:376	All the chitosan derivatives [N-trimethyl (TMC), chloride and glutamate] and carboxymethyl pullulan (CMP) were synthesised and antigen-loaded composites were prepared by polyion complexation of chitosan and pullulan derivatives (particle size: 239-405 nm; surface charge: +18 and +27 mV).
26480962	0	21	theme	composite	33:41	arg1	nanoparticles					43:55	chitosan-pullulan composite nanoparticles	15:55	chitosan-pullulan composite nanoparticles	15:55	Development of chitosan-pullulan composite nanoparticles for nasal delivery of vaccines: in vivo studies.
26480962	6	22	theme	balanced	983:990	arg1	response					1000:1007	a balanced Th1/Th2 response	981:1007	a balanced Th1/Th2 response	981:1007	Co-encapsulation of CpG ODN within TMC-CMP-DT nanoparticles resulted in a balanced Th1/Th2 response.
26480962	2	23	theme	surface	528:534	arg1	charge					536:541	surface charge	528:541	particle size: 239-405 nm; surface charge	501:541	All the chitosan derivatives [N-trimethyl (TMC), chloride and glutamate] and carboxymethyl pullulan (CMP) were synthesised and antigen-loaded composites were prepared by polyion complexation of chitosan and pullulan derivatives (particle size: 239-405 nm; surface charge: +18 and +27 mV).
26480962	7	24	theme	composite	1023:1031	arg1	nanoparticles					1033:1045	TMC/pullulan composite nanoparticles	1010:1045	TMC/pullulan composite nanoparticles	1010:1045	TMC/pullulan composite nanoparticles also induced highest cytokine levels compared to those of chitosan salts.
26480962	5	25	theme	higher	842:847	arg1	titre					862:866	higher DT serum IgG titre	842:866	higher DT serum IgG titre	842:866	Nasally administered TMC-pullulan composites showed higher DT serum IgG titre when compared with the other composites.
26480962	0	26	theme	chitosan-pullulan	15:31	arg1	nanoparticles					43:55	chitosan-pullulan composite nanoparticles	15:55	chitosan-pullulan composite nanoparticles	15:55	Development of chitosan-pullulan composite nanoparticles for nasal delivery of vaccines: in vivo studies.
26480962	2	27	theme	chitosan	280:287	arg1	derivatives					289:299	the chitosan derivatives	276:299	All the chitosan derivatives [N-trimethyl (TMC), chloride and glutamate]	272:343	All the chitosan derivatives [N-trimethyl (TMC), chloride and glutamate] and carboxymethyl pullulan (CMP) were synthesised and antigen-loaded composites were prepared by polyion complexation of chitosan and pullulan derivatives (particle size: 239-405 nm; surface charge: +18 and +27 mV).
26480962	2	27	theme	chitosan	280:287	arg1	chloride					321:328	chloride	321:328	chloride	321:328	All the chitosan derivatives [N-trimethyl (TMC), chloride and glutamate] and carboxymethyl pullulan (CMP) were synthesised and antigen-loaded composites were prepared by polyion complexation of chitosan and pullulan derivatives (particle size: 239-405 nm; surface charge: +18 and +27 mV).
26480962	2	27	theme	chitosan	280:287	arg1	[N-trimethyl					301:312	[N-trimethyl	301:312	[N-trimethyl (TMC)	301:318	All the chitosan derivatives [N-trimethyl (TMC), chloride and glutamate] and carboxymethyl pullulan (CMP) were synthesised and antigen-loaded composites were prepared by polyion complexation of chitosan and pullulan derivatives (particle size: 239-405 nm; surface charge: +18 and +27 mV).
26480962	2	27	theme	chitosan	280:287	arg1	glutamate					334:342	glutamate	334:342	glutamate	334:342	All the chitosan derivatives [N-trimethyl (TMC), chloride and glutamate] and carboxymethyl pullulan (CMP) were synthesised and antigen-loaded composites were prepared by polyion complexation of chitosan and pullulan derivatives (particle size: 239-405 nm; surface charge: +18 and +27 mV).
26480962	1	28	theme	novel	208:212	arg1	systems					214:220	novel systems	208:220	novel systems	208:220	Here, we aimed at developing chitosan/pullulan composite nanoparticles and testing their potential as novel systems for the nasal delivery of diphtheria toxoid (DT).
26480962	5	29	theme	serum	852:856	arg1	titre					862:866	higher DT serum IgG titre	842:866	higher DT serum IgG titre	842:866	Nasally administered TMC-pullulan composites showed higher DT serum IgG titre when compared with the other composites.
26480962	0	30	theme	nanoparticles	43:55	arg1	Development					0:10	Development	0:10	Development of chitosan-pullulan composite nanoparticles for nasal delivery of vaccines: in vivo studies.	0:104	Development of chitosan-pullulan composite nanoparticles for nasal delivery of vaccines: in vivo studies.
26480962	2	31	dep	size	510:513	arg1	mV					556:557	+18 and +27 mV	544:557	+18 and +27 mV	544:557	All the chitosan derivatives [N-trimethyl (TMC), chloride and glutamate] and carboxymethyl pullulan (CMP) were synthesised and antigen-loaded composites were prepared by polyion complexation of chitosan and pullulan derivatives (particle size: 239-405 nm; surface charge: +18 and +27 mV).
26480962	2	31	dep	size	510:513	arg1	charge					536:541	surface charge	528:541	particle size: 239-405 nm; surface charge	501:541	All the chitosan derivatives [N-trimethyl (TMC), chloride and glutamate] and carboxymethyl pullulan (CMP) were synthesised and antigen-loaded composites were prepared by polyion complexation of chitosan and pullulan derivatives (particle size: 239-405 nm; surface charge: +18 and +27 mV).
26480962	2	31	dep	size	510:513	arg1	nm					524:525	239-405 nm	516:525	particle size: 239-405 nm; surface charge	501:541	All the chitosan derivatives [N-trimethyl (TMC), chloride and glutamate] and carboxymethyl pullulan (CMP) were synthesised and antigen-loaded composites were prepared by polyion complexation of chitosan and pullulan derivatives (particle size: 239-405 nm; surface charge: +18 and +27 mV).
26480962	3	32	theme	intranasal	595:604	arg1	administration					606:619	intranasal administration	595:619	intranasal administration to mice	595:627	Their immunological effects after intranasal administration to mice were compared to intramuscular route.
26480962	5	33	theme	IgG	858:860	arg1	titre					862:866	higher DT serum IgG titre	842:866	higher DT serum IgG titre	842:866	Nasally administered TMC-pullulan composites showed higher DT serum IgG titre when compared with the other composites.
26480962	2	34	theme	polyion	442:448	arg1	complexation					450:461	polyion complexation	442:461	polyion complexation of chitosan and pullulan derivatives (particle size: 239-405 nm; surface charge: +18 and +27 mV)	442:558	All the chitosan derivatives [N-trimethyl (TMC), chloride and glutamate] and carboxymethyl pullulan (CMP) were synthesised and antigen-loaded composites were prepared by polyion complexation of chitosan and pullulan derivatives (particle size: 239-405 nm; surface charge: +18 and +27 mV).
26480962	8	35	theme	nasal	1223:1227	arg1	vaccination					1229:1239	nasal vaccination	1223:1239	nasal vaccination	1223:1239	These findings demonstrated that TMC-CMP-DT composite nanoparticles are promising delivery system for nasal vaccination.
26480962	8	36	theme	TMC-CMP-DT	1154:1163	arg1	system					1212:1217	promising delivery system	1193:1217	promising delivery system for nasal vaccination	1193:1239	These findings demonstrated that TMC-CMP-DT composite nanoparticles are promising delivery system for nasal vaccination.
26480962	8	36	theme	TMC-CMP-DT	1154:1163	arg1	nanoparticles					1175:1187	TMC-CMP-DT composite nanoparticles	1154:1187	TMC-CMP-DT composite nanoparticles	1154:1187	These findings demonstrated that TMC-CMP-DT composite nanoparticles are promising delivery system for nasal vaccination.
26480962	7	37	theme	highest	1060:1066	arg1	levels					1077:1082	highest cytokine levels	1060:1082	highest cytokine levels	1060:1082	TMC/pullulan composite nanoparticles also induced highest cytokine levels compared to those of chitosan salts.
26480962	2	38	dep	derivatives	289:299	arg1	glutamate					334:342	glutamate	334:342	glutamate	334:342	All the chitosan derivatives [N-trimethyl (TMC), chloride and glutamate] and carboxymethyl pullulan (CMP) were synthesised and antigen-loaded composites were prepared by polyion complexation of chitosan and pullulan derivatives (particle size: 239-405 nm; surface charge: +18 and +27 mV).
26480962	2	38	dep	derivatives	289:299	arg1	TMC					315:317	TMC	315:317	TMC	315:317	All the chitosan derivatives [N-trimethyl (TMC), chloride and glutamate] and carboxymethyl pullulan (CMP) were synthesised and antigen-loaded composites were prepared by polyion complexation of chitosan and pullulan derivatives (particle size: 239-405 nm; surface charge: +18 and +27 mV).
26480962	2	38	dep	derivatives	289:299	arg1	chloride					321:328	chloride	321:328	chloride	321:328	All the chitosan derivatives [N-trimethyl (TMC), chloride and glutamate] and carboxymethyl pullulan (CMP) were synthesised and antigen-loaded composites were prepared by polyion complexation of chitosan and pullulan derivatives (particle size: 239-405 nm; surface charge: +18 and +27 mV).
26480962	2	38	dep	derivatives	289:299	arg1	[N-trimethyl					301:312	[N-trimethyl	301:312	[N-trimethyl (TMC)	301:318	All the chitosan derivatives [N-trimethyl (TMC), chloride and glutamate] and carboxymethyl pullulan (CMP) were synthesised and antigen-loaded composites were prepared by polyion complexation of chitosan and pullulan derivatives (particle size: 239-405 nm; surface charge: +18 and +27 mV).
26480962	2	38	dep	derivatives	289:299	arg1	derivatives					289:299	the chitosan derivatives	276:299	All the chitosan derivatives [N-trimethyl (TMC), chloride and glutamate]	272:343	All the chitosan derivatives [N-trimethyl (TMC), chloride and glutamate] and carboxymethyl pullulan (CMP) were synthesised and antigen-loaded composites were prepared by polyion complexation of chitosan and pullulan derivatives (particle size: 239-405 nm; surface charge: +18 and +27 mV).
26480962	0	39	theme	nasal	61:65	arg1	delivery					67:74	nasal delivery	61:74	nasal delivery of vaccines	61:86	Development of chitosan-pullulan composite nanoparticles for nasal delivery of vaccines: in vivo studies.
26480962	6	40	theme	TMC-CMP-DT	944:953	arg1	nanoparticles					955:967	TMC-CMP-DT nanoparticles	944:967	TMC-CMP-DT nanoparticles	944:967	Co-encapsulation of CpG ODN within TMC-CMP-DT nanoparticles resulted in a balanced Th1/Th2 response.
26480962	7	41	theme	chitosan	1105:1112	arg1	salts					1114:1118	chitosan salts	1105:1118	chitosan salts	1105:1118	TMC/pullulan composite nanoparticles also induced highest cytokine levels compared to those of chitosan salts.
26480962	3	42	theme	immunological	567:579	arg1	effects					581:587	Their immunological effects	561:587	Their immunological effects after intranasal administration to mice	561:627	Their immunological effects after intranasal administration to mice were compared to intramuscular route.
26480962	7	43	theme	cytokine	1068:1075	arg1	levels					1077:1082	highest cytokine levels	1060:1082	highest cytokine levels	1060:1082	TMC/pullulan composite nanoparticles also induced highest cytokine levels compared to those of chitosan salts.
26480962	8	44	theme	composite	1165:1173	arg1	system					1212:1217	promising delivery system	1193:1217	promising delivery system for nasal vaccination	1193:1239	These findings demonstrated that TMC-CMP-DT composite nanoparticles are promising delivery system for nasal vaccination.
26480962	8	44	theme	composite	1165:1173	arg1	nanoparticles					1175:1187	TMC-CMP-DT composite nanoparticles	1154:1187	TMC-CMP-DT composite nanoparticles	1154:1187	These findings demonstrated that TMC-CMP-DT composite nanoparticles are promising delivery system for nasal vaccination.
26480962	7	45	theme	TMC/pullulan	1010:1021	arg1	nanoparticles					1033:1045	TMC/pullulan composite nanoparticles	1010:1045	TMC/pullulan composite nanoparticles	1010:1045	TMC/pullulan composite nanoparticles also induced highest cytokine levels compared to those of chitosan salts.
26480962	5	46	theme	other	891:895	arg1	composites					897:906	the other composites	887:906	the other composites	887:906	Nasally administered TMC-pullulan composites showed higher DT serum IgG titre when compared with the other composites.
26480962	1	47	theme	nasal	230:234	arg1	delivery					236:243	the nasal delivery	226:243	the nasal delivery of diphtheria toxoid (DT)	226:269	Here, we aimed at developing chitosan/pullulan composite nanoparticles and testing their potential as novel systems for the nasal delivery of diphtheria toxoid (DT).
26480962	0	48	theme	vaccines	79:86	arg1	delivery					67:74	nasal delivery	61:74	nasal delivery of vaccines	61:86	Development of chitosan-pullulan composite nanoparticles for nasal delivery of vaccines: in vivo studies.
26480962	2	49	theme	antigen-loaded	399:412	arg1	composites					414:423	antigen-loaded composites	399:423	antigen-loaded composites	399:423	All the chitosan derivatives [N-trimethyl (TMC), chloride and glutamate] and carboxymethyl pullulan (CMP) were synthesised and antigen-loaded composites were prepared by polyion complexation of chitosan and pullulan derivatives (particle size: 239-405 nm; surface charge: +18 and +27 mV).
26480962	2	50	theme	pullulan	479:486	arg1	derivatives					488:498	pullulan derivatives	479:498	pullulan derivatives (particle size: 239-405 nm; surface charge: +18 and +27 mV)	479:558	All the chitosan derivatives [N-trimethyl (TMC), chloride and glutamate] and carboxymethyl pullulan (CMP) were synthesised and antigen-loaded composites were prepared by polyion complexation of chitosan and pullulan derivatives (particle size: 239-405 nm; surface charge: +18 and +27 mV).
26480962	2	50	theme	pullulan	479:486	arg1	size					510:513	particle size	501:513	particle size: 239-405 nm; surface charge	501:541	All the chitosan derivatives [N-trimethyl (TMC), chloride and glutamate] and carboxymethyl pullulan (CMP) were synthesised and antigen-loaded composites were prepared by polyion complexation of chitosan and pullulan derivatives (particle size: 239-405 nm; surface charge: +18 and +27 mV).
26480962	6	51	theme	ODN	933:935	arg1	Co-encapsulation					909:924	Co-encapsulation	909:924	Co-encapsulation of CpG ODN within TMC-CMP-DT nanoparticles	909:967	Co-encapsulation of CpG ODN within TMC-CMP-DT nanoparticles resulted in a balanced Th1/Th2 response.
26480962	0	52	dep	Development	0:10	arg1	studies					97:103	in vivo studies	89:103	Development of chitosan-pullulan composite nanoparticles for nasal delivery of vaccines: in vivo studies.	0:104	Development of chitosan-pullulan composite nanoparticles for nasal delivery of vaccines: in vivo studies.
26480962	1	53	theme	chitosan/pullulan	135:151	arg1	nanoparticles					163:175	chitosan/pullulan composite nanoparticles	135:175	chitosan/pullulan composite nanoparticles	135:175	Here, we aimed at developing chitosan/pullulan composite nanoparticles and testing their potential as novel systems for the nasal delivery of diphtheria toxoid (DT).
27666435	6	0	theme	%	1152:1152	arg1	range					1134:1138	the range	1130:1138	the range of 0.74-8.29% and 82.33-117.86% respectively	1130:1183	Precision (% RSD) and accuracy (% recovery) for both methods were in the range of 0.74-8.29% and 82.33-117.86% respectively.
27666435	4	1	theme	water	860:864	arg1	elution					836:842	gradient elution	827:842	gradient elution of methanol and water (0.1% formic acid)	827:883	For HPLC-MS/MS method, separation was achieved using a Hypersil BDS C18 column with gradient elution of methanol and water (0.1% formic acid).
27666435	8	2	theme	pneumococcal	1385:1396	arg1	polysaccharides					1398:1412	purified pneumococcal polysaccharides	1376:1412	purified pneumococcal polysaccharides of serotypes: 1, 5, 6A, 6B, 7F, 9V, 14, 19A, 19F and 23F	1376:1469	Both methods enabled traceable quantitation of DOC in intermediate and purified pneumococcal polysaccharides of serotypes: 1, 5, 6A, 6B, 7F, 9V, 14, 19A, 19F and 23F.
27666435	1	3	theme	residual	168:175	arg1	DOC					198:200	DOC	198:200	DOC	198:200	The analysis of residual sodium deoxycholate (DOC); a detergent of biological origin used in manufacturing of polysaccharide vaccines is challenging due to complex sample matrices and the lack of suitable methods.
27666435	1	3	theme	residual	168:175	arg1	deoxycholate					184:195	residual sodium deoxycholate	168:195	residual sodium deoxycholate (DOC); a detergent of biological origin used in manufacturing of polysaccharide vaccines	168:284	The analysis of residual sodium deoxycholate (DOC); a detergent of biological origin used in manufacturing of polysaccharide vaccines is challenging due to complex sample matrices and the lack of suitable methods.
27666435	3	4	theme	%	735:735	arg1	acetate					718:724	20 mM sodium acetate	705:724	Luna C18 column and mobile phase compositions of acetonitrile: methanol: 20 mM sodium acetate (60:05:35% v/v)	632:740	For HPLC-RI method, separation was achieved using Luna C18 column and mobile phase compositions of acetonitrile: methanol: 20 mM sodium acetate (60:05:35% v/v).
27666435	3	4	theme	%	735:735	arg1	v/v					737:739	60:05:35% v/v	727:739	60:05:35% v/v	727:739	For HPLC-RI method, separation was achieved using Luna C18 column and mobile phase compositions of acetonitrile: methanol: 20 mM sodium acetate (60:05:35% v/v).
27666435	2	5	theme	pneumococcal	552:563	arg1	polysaccharides					565:579	pneumococcal polysaccharides	552:579	pneumococcal polysaccharides	552:579	Here we report, rapid and sensitive high-performance liquid chromatography-refractive index (HPLC-RI) and tandem mass spectrometry (HPLC-MS/MS) methods for estimation of residual DOC in pneumococcal polysaccharides.
27666435	1	6	theme	sodium	177:182	arg1	DOC					198:200	DOC	198:200	DOC	198:200	The analysis of residual sodium deoxycholate (DOC); a detergent of biological origin used in manufacturing of polysaccharide vaccines is challenging due to complex sample matrices and the lack of suitable methods.
27666435	1	6	theme	sodium	177:182	arg1	deoxycholate					184:195	residual sodium deoxycholate	168:195	residual sodium deoxycholate (DOC); a detergent of biological origin used in manufacturing of polysaccharide vaccines	168:284	The analysis of residual sodium deoxycholate (DOC); a detergent of biological origin used in manufacturing of polysaccharide vaccines is challenging due to complex sample matrices and the lack of suitable methods.
27666435	5	7	theme	quantitation	1010:1021	arg1	limits					965:970	limits	965:970	limits of detection (LOD)	965:989	MS/MS method showed linearity (r2 = 0.997) over the range of 10-320 ng/mL with limits of detection (LOD) and lower limit of quantitation (LOQ) of 3 and 10 ng/mL respectively.
27666435	5	7	theme	quantitation	1010:1021	arg1	limit					1001:1005	lower limit	995:1005	lower limit of quantitation (LOQ) of 3 and 10 ng/mL respectively	995:1058	MS/MS method showed linearity (r2 = 0.997) over the range of 10-320 ng/mL with limits of detection (LOD) and lower limit of quantitation (LOQ) of 3 and 10 ng/mL respectively.
27666435	0	8	theme	deoxycholate	99:110	arg1	levels					112:117	residual deoxycholate levels	90:117	residual deoxycholate levels in pneumococcal polysaccharides	90:149	Development and application of HPLC-RI and HPLC-MS/MS based methods for quantification of residual deoxycholate levels in pneumococcal polysaccharides.
27666435	1	9	theme	suitable	348:355	arg1	methods					357:363	suitable methods	348:363	suitable methods	348:363	The analysis of residual sodium deoxycholate (DOC); a detergent of biological origin used in manufacturing of polysaccharide vaccines is challenging due to complex sample matrices and the lack of suitable methods.
27666435	4	10	theme	0.1	867:869	arg1	%					870:870	%	870:870	%	870:870	For HPLC-MS/MS method, separation was achieved using a Hypersil BDS C18 column with gradient elution of methanol and water (0.1% formic acid).
27666435	7	11	theme	liquid-liquid	1279:1291	arg1	extraction					1293:1302	liquid-liquid extraction	1279:1302	liquid-liquid extraction	1279:1302	Sample matrices interferences were addressed following novel sample clean-up method based on liquid-liquid extraction.
27666435	1	12	theme	polysaccharide	262:275	arg1	vaccines					277:284	polysaccharide vaccines	262:284	polysaccharide vaccines	262:284	The analysis of residual sodium deoxycholate (DOC); a detergent of biological origin used in manufacturing of polysaccharide vaccines is challenging due to complex sample matrices and the lack of suitable methods.
27666435	0	13	theme	residual	90:97	arg1	levels					112:117	residual deoxycholate levels	90:117	residual deoxycholate levels in pneumococcal polysaccharides	90:149	Development and application of HPLC-RI and HPLC-MS/MS based methods for quantification of residual deoxycholate levels in pneumococcal polysaccharides.
27666435	1	14	theme	methods	357:363	arg1	matrices					323:330	complex sample matrices	308:330	complex sample matrices	308:330	The analysis of residual sodium deoxycholate (DOC); a detergent of biological origin used in manufacturing of polysaccharide vaccines is challenging due to complex sample matrices and the lack of suitable methods.
27666435	1	14	theme	methods	357:363	arg1	lack					340:343	the lack	336:343	the lack of suitable methods	336:363	The analysis of residual sodium deoxycholate (DOC); a detergent of biological origin used in manufacturing of polysaccharide vaccines is challenging due to complex sample matrices and the lack of suitable methods.
27666435	8	15	from	quantitation	1336:1347	arg1	polysaccharides					1398:1412	purified pneumococcal polysaccharides	1376:1412	purified pneumococcal polysaccharides of serotypes: 1, 5, 6A, 6B, 7F, 9V, 14, 19A, 19F and 23F	1376:1469	Both methods enabled traceable quantitation of DOC in intermediate and purified pneumococcal polysaccharides of serotypes: 1, 5, 6A, 6B, 7F, 9V, 14, 19A, 19F and 23F.
27666435	8	15	from	quantitation	1336:1347	arg1	intermediate					1359:1370	intermediate	1359:1370	intermediate	1359:1370	Both methods enabled traceable quantitation of DOC in intermediate and purified pneumococcal polysaccharides of serotypes: 1, 5, 6A, 6B, 7F, 9V, 14, 19A, 19F and 23F.
27666435	1	16	theme	vaccines	277:284	arg1	manufacturing					245:257	manufacturing	245:257	manufacturing of polysaccharide vaccines	245:284	The analysis of residual sodium deoxycholate (DOC); a detergent of biological origin used in manufacturing of polysaccharide vaccines is challenging due to complex sample matrices and the lack of suitable methods.
27666435	5	17	theme	3	1032:1032	arg1	LOQ					1024:1026	LOQ	1024:1026	LOQ	1024:1026	MS/MS method showed linearity (r2 = 0.997) over the range of 10-320 ng/mL with limits of detection (LOD) and lower limit of quantitation (LOQ) of 3 and 10 ng/mL respectively.
27666435	5	17	theme	3	1032:1032	arg1	quantitation					1010:1021	quantitation	1010:1021	quantitation (LOQ) of 3 and 10 ng/mL respectively	1010:1058	MS/MS method showed linearity (r2 = 0.997) over the range of 10-320 ng/mL with limits of detection (LOD) and lower limit of quantitation (LOQ) of 3 and 10 ng/mL respectively.
27666435	6	18	dep	accuracy	1083:1090	arg1	%					1093:1093	%	1093:1093	% recovery	1093:1102	Precision (% RSD) and accuracy (% recovery) for both methods were in the range of 0.74-8.29% and 82.33-117.86% respectively.
27666435	1	19	theme	deoxycholate	184:195	arg1	analysis					156:163	The analysis	152:163	The analysis of residual sodium deoxycholate (DOC); a detergent of biological origin used in manufacturing of polysaccharide vaccines	152:284	The analysis of residual sodium deoxycholate (DOC); a detergent of biological origin used in manufacturing of polysaccharide vaccines is challenging due to complex sample matrices and the lack of suitable methods.
27666435	0	20	theme	levels	112:117	arg1	quantification					72:85	quantification	72:85	quantification of residual deoxycholate levels in pneumococcal polysaccharides	72:149	Development and application of HPLC-RI and HPLC-MS/MS based methods for quantification of residual deoxycholate levels in pneumococcal polysaccharides.
27666435	4	21	theme	gradient	827:834	arg1	elution					836:842	gradient elution	827:842	gradient elution of methanol and water (0.1% formic acid)	827:883	For HPLC-MS/MS method, separation was achieved using a Hypersil BDS C18 column with gradient elution of methanol and water (0.1% formic acid).
27666435	2	22	theme	rapid	382:386	arg1	methods					510:516	rapid and sensitive high-performance liquid chromatography-refractive index (HPLC-RI) and tandem mass spectrometry (HPLC-MS/MS) methods	382:516	rapid and sensitive high-performance liquid chromatography-refractive index (HPLC-RI) and tandem mass spectrometry (HPLC-MS/MS) methods for estimation of residual DOC in pneumococcal polysaccharides	382:579	Here we report, rapid and sensitive high-performance liquid chromatography-refractive index (HPLC-RI) and tandem mass spectrometry (HPLC-MS/MS) methods for estimation of residual DOC in pneumococcal polysaccharides.
27666435	5	23	theme	10 ng/mL	1038:1045	arg1	LOQ					1024:1026	LOQ	1024:1026	LOQ	1024:1026	MS/MS method showed linearity (r2 = 0.997) over the range of 10-320 ng/mL with limits of detection (LOD) and lower limit of quantitation (LOQ) of 3 and 10 ng/mL respectively.
27666435	5	23	theme	10 ng/mL	1038:1045	arg1	quantitation					1010:1021	quantitation	1010:1021	quantitation (LOQ) of 3 and 10 ng/mL respectively	1010:1058	MS/MS method showed linearity (r2 = 0.997) over the range of 10-320 ng/mL with limits of detection (LOD) and lower limit of quantitation (LOQ) of 3 and 10 ng/mL respectively.
27666435	4	24	theme	methanol	847:854	arg1	elution					836:842	gradient elution	827:842	gradient elution of methanol and water (0.1% formic acid)	827:883	For HPLC-MS/MS method, separation was achieved using a Hypersil BDS C18 column with gradient elution of methanol and water (0.1% formic acid).
27666435	3	25	theme	HPLC-RI	586:592	arg1	method					594:599	HPLC-RI method	586:599	HPLC-RI method	586:599	For HPLC-RI method, separation was achieved using Luna C18 column and mobile phase compositions of acetonitrile: methanol: 20 mM sodium acetate (60:05:35% v/v).
27666435	3	26	theme	20 mM	705:709	arg1	acetate					718:724	20 mM sodium acetate	705:724	Luna C18 column and mobile phase compositions of acetonitrile: methanol: 20 mM sodium acetate (60:05:35% v/v)	632:740	For HPLC-RI method, separation was achieved using Luna C18 column and mobile phase compositions of acetonitrile: methanol: 20 mM sodium acetate (60:05:35% v/v).
27666435	3	26	theme	20 mM	705:709	arg1	v/v					737:739	60:05:35% v/v	727:739	60:05:35% v/v	727:739	For HPLC-RI method, separation was achieved using Luna C18 column and mobile phase compositions of acetonitrile: methanol: 20 mM sodium acetate (60:05:35% v/v).
27666435	2	27	theme	residual	536:543	arg1	DOC					545:547	residual DOC	536:547	residual DOC	536:547	Here we report, rapid and sensitive high-performance liquid chromatography-refractive index (HPLC-RI) and tandem mass spectrometry (HPLC-MS/MS) methods for estimation of residual DOC in pneumococcal polysaccharides.
27666435	8	28	theme	serotypes	1417:1425	arg1	polysaccharides					1398:1412	purified pneumococcal polysaccharides	1376:1412	purified pneumococcal polysaccharides of serotypes: 1, 5, 6A, 6B, 7F, 9V, 14, 19A, 19F and 23F	1376:1469	Both methods enabled traceable quantitation of DOC in intermediate and purified pneumococcal polysaccharides of serotypes: 1, 5, 6A, 6B, 7F, 9V, 14, 19A, 19F and 23F.
27666435	8	28	theme	serotypes	1417:1425	arg1	intermediate					1359:1370	intermediate	1359:1370	intermediate	1359:1370	Both methods enabled traceable quantitation of DOC in intermediate and purified pneumococcal polysaccharides of serotypes: 1, 5, 6A, 6B, 7F, 9V, 14, 19A, 19F and 23F.
27666435	7	29	theme	Sample	1186:1191	arg1	interferences					1202:1214	Sample matrices interferences	1186:1214	Sample matrices interferences	1186:1214	Sample matrices interferences were addressed following novel sample clean-up method based on liquid-liquid extraction.
27666435	7	30	theme	clean-up	1254:1261	arg1	method					1263:1268	novel sample clean-up method	1241:1268	novel sample clean-up method based on liquid-liquid extraction	1241:1302	Sample matrices interferences were addressed following novel sample clean-up method based on liquid-liquid extraction.
27666435	6	31	dep	%	1093:1093	arg1	recovery					1095:1102	recovery	1095:1102	% recovery	1093:1102	Precision (% RSD) and accuracy (% recovery) for both methods were in the range of 0.74-8.29% and 82.33-117.86% respectively.
27666435	8	32	theme	traceable	1326:1334	arg1	quantitation					1336:1347	traceable quantitation	1326:1347	traceable quantitation of DOC in intermediate and purified pneumococcal polysaccharides of serotypes: 1, 5, 6A, 6B, 7F, 9V, 14, 19A, 19F and 23F	1326:1469	Both methods enabled traceable quantitation of DOC in intermediate and purified pneumococcal polysaccharides of serotypes: 1, 5, 6A, 6B, 7F, 9V, 14, 19A, 19F and 23F.
27666435	4	33	theme	HPLC-MS/MS	747:756	arg1	method					758:763	HPLC-MS/MS method	747:763	HPLC-MS/MS method	747:763	For HPLC-MS/MS method, separation was achieved using a Hypersil BDS C18 column with gradient elution of methanol and water (0.1% formic acid).
27666435	5	34	theme	detection	975:983	arg1	limits					965:970	limits	965:970	limits of detection (LOD)	965:989	MS/MS method showed linearity (r2 = 0.997) over the range of 10-320 ng/mL with limits of detection (LOD) and lower limit of quantitation (LOQ) of 3 and 10 ng/mL respectively.
27666435	5	34	theme	detection	975:983	arg1	limit					1001:1005	lower limit	995:1005	lower limit of quantitation (LOQ) of 3 and 10 ng/mL respectively	995:1058	MS/MS method showed linearity (r2 = 0.997) over the range of 10-320 ng/mL with limits of detection (LOD) and lower limit of quantitation (LOQ) of 3 and 10 ng/mL respectively.
27666435	2	35	theme	chromatography-refractive	426:450	arg1	HPLC-RI					459:465	HPLC-RI	459:465	HPLC-RI	459:465	Here we report, rapid and sensitive high-performance liquid chromatography-refractive index (HPLC-RI) and tandem mass spectrometry (HPLC-MS/MS) methods for estimation of residual DOC in pneumococcal polysaccharides.
27666435	2	35	theme	chromatography-refractive	426:450	arg1	index					452:456	high-performance liquid chromatography-refractive index	402:456	high-performance liquid chromatography-refractive index (HPLC-RI)	402:466	Here we report, rapid and sensitive high-performance liquid chromatography-refractive index (HPLC-RI) and tandem mass spectrometry (HPLC-MS/MS) methods for estimation of residual DOC in pneumococcal polysaccharides.
27666435	2	36	theme	index	452:456	arg1	methods					510:516	rapid and sensitive high-performance liquid chromatography-refractive index (HPLC-RI) and tandem mass spectrometry (HPLC-MS/MS) methods	382:516	rapid and sensitive high-performance liquid chromatography-refractive index (HPLC-RI) and tandem mass spectrometry (HPLC-MS/MS) methods for estimation of residual DOC in pneumococcal polysaccharides	382:579	Here we report, rapid and sensitive high-performance liquid chromatography-refractive index (HPLC-RI) and tandem mass spectrometry (HPLC-MS/MS) methods for estimation of residual DOC in pneumococcal polysaccharides.
27666435	8	37	dep	serotypes	1417:1425	arg1	serotypes					1417:1425	serotypes	1417:1425	serotypes: 1, 5, 6A, 6B, 7F, 9V, 14, 19A, 19F and 23F	1417:1469	Both methods enabled traceable quantitation of DOC in intermediate and purified pneumococcal polysaccharides of serotypes: 1, 5, 6A, 6B, 7F, 9V, 14, 19A, 19F and 23F.
27666435	8	37	dep	serotypes	1417:1425	arg1	23F					1467:1469	23F	1467:1469	23F	1467:1469	Both methods enabled traceable quantitation of DOC in intermediate and purified pneumococcal polysaccharides of serotypes: 1, 5, 6A, 6B, 7F, 9V, 14, 19A, 19F and 23F.
27666435	8	37	dep	serotypes	1417:1425	arg1	19F					1459:1461	19F	1459:1461	19F	1459:1461	Both methods enabled traceable quantitation of DOC in intermediate and purified pneumococcal polysaccharides of serotypes: 1, 5, 6A, 6B, 7F, 9V, 14, 19A, 19F and 23F.
27666435	0	38	theme	pneumococcal	122:133	arg1	polysaccharides					135:149	pneumococcal polysaccharides	122:149	pneumococcal polysaccharides	122:149	Development and application of HPLC-RI and HPLC-MS/MS based methods for quantification of residual deoxycholate levels in pneumococcal polysaccharides.
27666435	3	39	theme	phase	659:663	arg1	compositions					665:676	mobile phase compositions	652:676	mobile phase compositions	652:676	For HPLC-RI method, separation was achieved using Luna C18 column and mobile phase compositions of acetonitrile: methanol: 20 mM sodium acetate (60:05:35% v/v).
27666435	6	40	theme	%	1072:1072	arg1	Precision					1061:1069	Precision	1061:1069	Precision (% RSD)	1061:1077	Precision (% RSD) and accuracy (% recovery) for both methods were in the range of 0.74-8.29% and 82.33-117.86% respectively.
27666435	6	40	theme	%	1072:1072	arg1	RSD					1074:1076	% RSD	1072:1076	% RSD	1072:1076	Precision (% RSD) and accuracy (% recovery) for both methods were in the range of 0.74-8.29% and 82.33-117.86% respectively.
27666435	2	41	theme	liquid	419:424	arg1	HPLC-RI					459:465	HPLC-RI	459:465	HPLC-RI	459:465	Here we report, rapid and sensitive high-performance liquid chromatography-refractive index (HPLC-RI) and tandem mass spectrometry (HPLC-MS/MS) methods for estimation of residual DOC in pneumococcal polysaccharides.
27666435	2	41	theme	liquid	419:424	arg1	index					452:456	high-performance liquid chromatography-refractive index	402:456	high-performance liquid chromatography-refractive index (HPLC-RI)	402:466	Here we report, rapid and sensitive high-performance liquid chromatography-refractive index (HPLC-RI) and tandem mass spectrometry (HPLC-MS/MS) methods for estimation of residual DOC in pneumococcal polysaccharides.
27666435	2	42	theme	spectrometry	484:495	arg1	methods					510:516	rapid and sensitive high-performance liquid chromatography-refractive index (HPLC-RI) and tandem mass spectrometry (HPLC-MS/MS) methods	382:516	rapid and sensitive high-performance liquid chromatography-refractive index (HPLC-RI) and tandem mass spectrometry (HPLC-MS/MS) methods for estimation of residual DOC in pneumococcal polysaccharides	382:579	Here we report, rapid and sensitive high-performance liquid chromatography-refractive index (HPLC-RI) and tandem mass spectrometry (HPLC-MS/MS) methods for estimation of residual DOC in pneumococcal polysaccharides.
27666435	7	43	theme	matrices	1193:1200	arg1	interferences					1202:1214	Sample matrices interferences	1186:1214	Sample matrices interferences	1186:1214	Sample matrices interferences were addressed following novel sample clean-up method based on liquid-liquid extraction.
27666435	0	44	from	quantification	72:85	arg1	polysaccharides					135:149	pneumococcal polysaccharides	122:149	pneumococcal polysaccharides	122:149	Development and application of HPLC-RI and HPLC-MS/MS based methods for quantification of residual deoxycholate levels in pneumococcal polysaccharides.
27666435	4	45	theme	C18	811:813	arg1	column					815:820	a Hypersil BDS C18 column	796:820	a Hypersil BDS C18 column	796:820	For HPLC-MS/MS method, separation was achieved using a Hypersil BDS C18 column with gradient elution of methanol and water (0.1% formic acid).
27666435	2	46	theme	high-performance	402:417	arg1	HPLC-RI					459:465	HPLC-RI	459:465	HPLC-RI	459:465	Here we report, rapid and sensitive high-performance liquid chromatography-refractive index (HPLC-RI) and tandem mass spectrometry (HPLC-MS/MS) methods for estimation of residual DOC in pneumococcal polysaccharides.
27666435	2	46	theme	high-performance	402:417	arg1	index					452:456	high-performance liquid chromatography-refractive index	402:456	high-performance liquid chromatography-refractive index (HPLC-RI)	402:466	Here we report, rapid and sensitive high-performance liquid chromatography-refractive index (HPLC-RI) and tandem mass spectrometry (HPLC-MS/MS) methods for estimation of residual DOC in pneumococcal polysaccharides.
27666435	0	47	theme	HPLC-RI	31:37	arg1	application					16:26	application	16:26	application	16:26	Development and application of HPLC-RI and HPLC-MS/MS based methods for quantification of residual deoxycholate levels in pneumococcal polysaccharides.
27666435	0	47	theme	HPLC-RI	31:37	arg1	Development					0:10	Development	0:10	Development	0:10	Development and application of HPLC-RI and HPLC-MS/MS based methods for quantification of residual deoxycholate levels in pneumococcal polysaccharides.
27666435	1	48	theme	complex	308:314	arg1	matrices					323:330	complex sample matrices	308:330	complex sample matrices	308:330	The analysis of residual sodium deoxycholate (DOC); a detergent of biological origin used in manufacturing of polysaccharide vaccines is challenging due to complex sample matrices and the lack of suitable methods.
27666435	2	49	theme	mass	479:482	arg1	HPLC-MS/MS					498:507	HPLC-MS/MS	498:507	HPLC-MS/MS	498:507	Here we report, rapid and sensitive high-performance liquid chromatography-refractive index (HPLC-RI) and tandem mass spectrometry (HPLC-MS/MS) methods for estimation of residual DOC in pneumococcal polysaccharides.
27666435	2	49	theme	mass	479:482	arg1	spectrometry					484:495	tandem mass spectrometry	472:495	tandem mass spectrometry (HPLC-MS/MS)	472:508	Here we report, rapid and sensitive high-performance liquid chromatography-refractive index (HPLC-RI) and tandem mass spectrometry (HPLC-MS/MS) methods for estimation of residual DOC in pneumococcal polysaccharides.
27666435	7	50	theme	novel	1241:1245	arg1	method					1263:1268	novel sample clean-up method	1241:1268	novel sample clean-up method based on liquid-liquid extraction	1241:1302	Sample matrices interferences were addressed following novel sample clean-up method based on liquid-liquid extraction.
27666435	4	51	theme	BDS	807:809	arg1	column					815:820	a Hypersil BDS C18 column	796:820	a Hypersil BDS C18 column	796:820	For HPLC-MS/MS method, separation was achieved using a Hypersil BDS C18 column with gradient elution of methanol and water (0.1% formic acid).
27666435	2	52	theme	DOC	545:547	arg1	estimation					522:531	estimation	522:531	estimation of residual DOC in pneumococcal polysaccharides	522:579	Here we report, rapid and sensitive high-performance liquid chromatography-refractive index (HPLC-RI) and tandem mass spectrometry (HPLC-MS/MS) methods for estimation of residual DOC in pneumococcal polysaccharides.
27666435	2	53	theme	sensitive	392:400	arg1	methods					510:516	rapid and sensitive high-performance liquid chromatography-refractive index (HPLC-RI) and tandem mass spectrometry (HPLC-MS/MS) methods	382:516	rapid and sensitive high-performance liquid chromatography-refractive index (HPLC-RI) and tandem mass spectrometry (HPLC-MS/MS) methods for estimation of residual DOC in pneumococcal polysaccharides	382:579	Here we report, rapid and sensitive high-performance liquid chromatography-refractive index (HPLC-RI) and tandem mass spectrometry (HPLC-MS/MS) methods for estimation of residual DOC in pneumococcal polysaccharides.
27666435	2	54	theme	tandem	472:477	arg1	HPLC-MS/MS					498:507	HPLC-MS/MS	498:507	HPLC-MS/MS	498:507	Here we report, rapid and sensitive high-performance liquid chromatography-refractive index (HPLC-RI) and tandem mass spectrometry (HPLC-MS/MS) methods for estimation of residual DOC in pneumococcal polysaccharides.
27666435	2	54	theme	tandem	472:477	arg1	spectrometry					484:495	tandem mass spectrometry	472:495	tandem mass spectrometry (HPLC-MS/MS)	472:508	Here we report, rapid and sensitive high-performance liquid chromatography-refractive index (HPLC-RI) and tandem mass spectrometry (HPLC-MS/MS) methods for estimation of residual DOC in pneumococcal polysaccharides.
27666435	1	55	theme	sample	316:321	arg1	matrices					323:330	complex sample matrices	308:330	complex sample matrices	308:330	The analysis of residual sodium deoxycholate (DOC); a detergent of biological origin used in manufacturing of polysaccharide vaccines is challenging due to complex sample matrices and the lack of suitable methods.
27666435	4	56	theme	formic	872:877	arg1	acid					879:882	0.1% formic acid	867:882	0.1% formic acid	867:882	For HPLC-MS/MS method, separation was achieved using a Hypersil BDS C18 column with gradient elution of methanol and water (0.1% formic acid).
27666435	4	56	theme	formic	872:877	arg1	methanol					847:854	methanol	847:854	methanol	847:854	For HPLC-MS/MS method, separation was achieved using a Hypersil BDS C18 column with gradient elution of methanol and water (0.1% formic acid).
27666435	3	57	theme	mobile	652:657	arg1	compositions					665:676	mobile phase compositions	652:676	mobile phase compositions	652:676	For HPLC-RI method, separation was achieved using Luna C18 column and mobile phase compositions of acetonitrile: methanol: 20 mM sodium acetate (60:05:35% v/v).
27666435	1	58	dep	deoxycholate	184:195	arg1	detergent					206:214	a detergent	204:214	residual sodium deoxycholate (DOC); a detergent of biological origin used in manufacturing of polysaccharide vaccines	168:284	The analysis of residual sodium deoxycholate (DOC); a detergent of biological origin used in manufacturing of polysaccharide vaccines is challenging due to complex sample matrices and the lack of suitable methods.
27666435	0	59	theme	HPLC-MS/MS	43:52	arg1	application					16:26	application	16:26	application	16:26	Development and application of HPLC-RI and HPLC-MS/MS based methods for quantification of residual deoxycholate levels in pneumococcal polysaccharides.
27666435	0	59	theme	HPLC-MS/MS	43:52	arg1	Development					0:10	Development	0:10	Development	0:10	Development and application of HPLC-RI and HPLC-MS/MS based methods for quantification of residual deoxycholate levels in pneumococcal polysaccharides.
27666435	4	60	theme	%	870:870	arg1	acid					879:882	0.1% formic acid	867:882	0.1% formic acid	867:882	For HPLC-MS/MS method, separation was achieved using a Hypersil BDS C18 column with gradient elution of methanol and water (0.1% formic acid).
27666435	4	60	theme	%	870:870	arg1	methanol					847:854	methanol	847:854	methanol	847:854	For HPLC-MS/MS method, separation was achieved using a Hypersil BDS C18 column with gradient elution of methanol and water (0.1% formic acid).
27666435	2	61	from	estimation	522:531	arg1	polysaccharides					565:579	pneumococcal polysaccharides	552:579	pneumococcal polysaccharides	552:579	Here we report, rapid and sensitive high-performance liquid chromatography-refractive index (HPLC-RI) and tandem mass spectrometry (HPLC-MS/MS) methods for estimation of residual DOC in pneumococcal polysaccharides.
27666435	5	62	theme	lower	995:999	arg1	limit					1001:1005	lower limit	995:1005	lower limit of quantitation (LOQ) of 3 and 10 ng/mL respectively	995:1058	MS/MS method showed linearity (r2 = 0.997) over the range of 10-320 ng/mL with limits of detection (LOD) and lower limit of quantitation (LOQ) of 3 and 10 ng/mL respectively.
27666435	5	63	theme	MS/MS	886:890	arg1	method					892:897	MS/MS method	886:897	MS/MS method	886:897	MS/MS method showed linearity (r2 = 0.997) over the range of 10-320 ng/mL with limits of detection (LOD) and lower limit of quantitation (LOQ) of 3 and 10 ng/mL respectively.
27666435	8	64	theme	DOC	1352:1354	arg1	quantitation					1336:1347	traceable quantitation	1326:1347	traceable quantitation of DOC in intermediate and purified pneumococcal polysaccharides of serotypes: 1, 5, 6A, 6B, 7F, 9V, 14, 19A, 19F and 23F	1326:1469	Both methods enabled traceable quantitation of DOC in intermediate and purified pneumococcal polysaccharides of serotypes: 1, 5, 6A, 6B, 7F, 9V, 14, 19A, 19F and 23F.
27666435	0	65	from	levels	112:117	arg1	polysaccharides					135:149	pneumococcal polysaccharides	122:149	pneumococcal polysaccharides	122:149	Development and application of HPLC-RI and HPLC-MS/MS based methods for quantification of residual deoxycholate levels in pneumococcal polysaccharides.
27666435	3	66	theme	C18	637:639	arg1	column					641:646	Luna C18 column	632:646	Luna C18 column	632:646	For HPLC-RI method, separation was achieved using Luna C18 column and mobile phase compositions of acetonitrile: methanol: 20 mM sodium acetate (60:05:35% v/v).
27666435	1	67	theme	biological	219:228	arg1	origin					230:235	biological origin	219:235	biological origin	219:235	The analysis of residual sodium deoxycholate (DOC); a detergent of biological origin used in manufacturing of polysaccharide vaccines is challenging due to complex sample matrices and the lack of suitable methods.
27666435	8	68	theme	purified	1376:1383	arg1	polysaccharides					1398:1412	purified pneumococcal polysaccharides	1376:1412	purified pneumococcal polysaccharides of serotypes: 1, 5, 6A, 6B, 7F, 9V, 14, 19A, 19F and 23F	1376:1469	Both methods enabled traceable quantitation of DOC in intermediate and purified pneumococcal polysaccharides of serotypes: 1, 5, 6A, 6B, 7F, 9V, 14, 19A, 19F and 23F.
27666435	8	69	theme	19A	1454:1456	arg1	serotypes					1417:1425	serotypes	1417:1425	serotypes: 1, 5, 6A, 6B, 7F, 9V, 14, 19A, 19F and 23F	1417:1469	Both methods enabled traceable quantitation of DOC in intermediate and purified pneumococcal polysaccharides of serotypes: 1, 5, 6A, 6B, 7F, 9V, 14, 19A, 19F and 23F.
27666435	8	69	theme	19A	1454:1456	arg1	19F					1459:1461	19F	1459:1461	19F	1459:1461	Both methods enabled traceable quantitation of DOC in intermediate and purified pneumococcal polysaccharides of serotypes: 1, 5, 6A, 6B, 7F, 9V, 14, 19A, 19F and 23F.
27666435	1	70	theme	origin	230:235	arg1	detergent					206:214	a detergent	204:214	residual sodium deoxycholate (DOC); a detergent of biological origin used in manufacturing of polysaccharide vaccines	168:284	The analysis of residual sodium deoxycholate (DOC); a detergent of biological origin used in manufacturing of polysaccharide vaccines is challenging due to complex sample matrices and the lack of suitable methods.
27666435	3	71	dep	column	641:646	arg1	v/v					737:739	60:05:35% v/v	727:739	60:05:35% v/v	727:739	For HPLC-RI method, separation was achieved using Luna C18 column and mobile phase compositions of acetonitrile: methanol: 20 mM sodium acetate (60:05:35% v/v).
27666435	3	71	dep	column	641:646	arg1	acetate					718:724	20 mM sodium acetate	705:724	Luna C18 column and mobile phase compositions of acetonitrile: methanol: 20 mM sodium acetate (60:05:35% v/v)	632:740	For HPLC-RI method, separation was achieved using Luna C18 column and mobile phase compositions of acetonitrile: methanol: 20 mM sodium acetate (60:05:35% v/v).
27666435	3	71	dep	column	641:646	arg1	methanol					695:702	methanol	695:702	Luna C18 column and mobile phase compositions of acetonitrile: methanol: 20 mM sodium acetate (60:05:35% v/v)	632:740	For HPLC-RI method, separation was achieved using Luna C18 column and mobile phase compositions of acetonitrile: methanol: 20 mM sodium acetate (60:05:35% v/v).
27666435	3	72	theme	acetonitrile	681:692	arg1	compositions					665:676	mobile phase compositions	652:676	mobile phase compositions	652:676	For HPLC-RI method, separation was achieved using Luna C18 column and mobile phase compositions of acetonitrile: methanol: 20 mM sodium acetate (60:05:35% v/v).
27666435	3	72	theme	acetonitrile	681:692	arg1	column					641:646	Luna C18 column	632:646	Luna C18 column	632:646	For HPLC-RI method, separation was achieved using Luna C18 column and mobile phase compositions of acetonitrile: methanol: 20 mM sodium acetate (60:05:35% v/v).
27666435	4	73	theme	Hypersil	798:805	arg1	column					815:820	a Hypersil BDS C18 column	796:820	a Hypersil BDS C18 column	796:820	For HPLC-MS/MS method, separation was achieved using a Hypersil BDS C18 column with gradient elution of methanol and water (0.1% formic acid).
27666435	7	74	theme	sample	1247:1252	arg1	method					1263:1268	novel sample clean-up method	1241:1268	novel sample clean-up method based on liquid-liquid extraction	1241:1302	Sample matrices interferences were addressed following novel sample clean-up method based on liquid-liquid extraction.
27666435	0	75	from	polysaccharides	135:149	arg1	quantification					72:85	quantification	72:85	quantification of residual deoxycholate levels in pneumococcal polysaccharides	72:149	Development and application of HPLC-RI and HPLC-MS/MS based methods for quantification of residual deoxycholate levels in pneumococcal polysaccharides.
27666435	5	76	theme	10-320 ng/mL	947:958	arg1	range					938:942	the range	934:942	the range of 10-320 ng/mL	934:958	MS/MS method showed linearity (r2 = 0.997) over the range of 10-320 ng/mL with limits of detection (LOD) and lower limit of quantitation (LOQ) of 3 and 10 ng/mL respectively.
27666435	3	77	theme	sodium	711:716	arg1	acetate					718:724	20 mM sodium acetate	705:724	Luna C18 column and mobile phase compositions of acetonitrile: methanol: 20 mM sodium acetate (60:05:35% v/v)	632:740	For HPLC-RI method, separation was achieved using Luna C18 column and mobile phase compositions of acetonitrile: methanol: 20 mM sodium acetate (60:05:35% v/v).
27666435	3	77	theme	sodium	711:716	arg1	v/v					737:739	60:05:35% v/v	727:739	60:05:35% v/v	727:739	For HPLC-RI method, separation was achieved using Luna C18 column and mobile phase compositions of acetonitrile: methanol: 20 mM sodium acetate (60:05:35% v/v).
27666435	3	78	theme	Luna	632:635	arg1	column					641:646	Luna C18 column	632:646	Luna C18 column	632:646	For HPLC-RI method, separation was achieved using Luna C18 column and mobile phase compositions of acetonitrile: methanol: 20 mM sodium acetate (60:05:35% v/v).
27666435	6	79	theme	%	1170:1170	arg1	range					1134:1138	the range	1130:1138	the range of 0.74-8.29% and 82.33-117.86% respectively	1130:1183	Precision (% RSD) and accuracy (% recovery) for both methods were in the range of 0.74-8.29% and 82.33-117.86% respectively.
28254732	5	0	from	sorghum	721:727	arg1	dominant					700:707	dominant	700:707	dominant	700:707	The aqueous fraction from sweet sorghum bagasse contained furfural and furan carboxyl aldehydes, while ketones and alcohols were dominant from energy sorghum as other key compounds.
28254732	2	1	theme	sorghum	304:310	arg1	bagasse					312:318	sweet sorghum bagasse	298:318	sweet sorghum bagasse	298:318	The torrefied solid yield ranged from 43% to 65% for sweet sorghum bagasse and 51-70% for energy sorghum.
28254732	4	2	from	compound	518:525	arg1	fraction					542:549	the aqueous fraction	530:549	the aqueous fraction of liquid products	530:568	Besides water, the acetic acid, with a maximum yield of 101.90gL-1 was the dominant compound in the aqueous fraction of liquid products.
28254732	6	3	theme	oil	831:833	arg1	fraction					835:842	the oil fraction	827:842	the oil fraction of the liquid product	827:864	Phenolic type chemicals and furan derivatives were the major compounds in the oil fraction of the liquid product, accounted up to 58wt%.
28254732	2	4	theme	sweet	298:302	arg1	bagasse					312:318	sweet sorghum bagasse	298:318	sweet sorghum bagasse	298:318	The torrefied solid yield ranged from 43% to 65% for sweet sorghum bagasse and 51-70% for energy sorghum.
28254732	4	5	theme	101.90gL-1	490:499	arg1	yield					481:485	a maximum yield	471:485	a maximum yield of 101.90gL-1	471:499	Besides water, the acetic acid, with a maximum yield of 101.90gL-1 was the dominant compound in the aqueous fraction of liquid products.
28254732	5	6	theme	carboxyl	648:655	arg1	aldehydes					657:665	furfural and furan carboxyl aldehydes	629:665	furfural and furan carboxyl aldehydes	629:665	The aqueous fraction from sweet sorghum bagasse contained furfural and furan carboxyl aldehydes, while ketones and alcohols were dominant from energy sorghum as other key compounds.
28254732	5	7	theme	aqueous	575:581	arg1	fraction					583:590	The aqueous fraction	571:590	The aqueous fraction from sweet sorghum bagasse	571:617	The aqueous fraction from sweet sorghum bagasse contained furfural and furan carboxyl aldehydes, while ketones and alcohols were dominant from energy sorghum as other key compounds.
28254732	2	8	dep	%	292:292	arg1	to					287:288	to	287:288	to	287:288	The torrefied solid yield ranged from 43% to 65% for sweet sorghum bagasse and 51-70% for energy sorghum.
28254732	4	9	theme	dominant	509:516	arg1	acid					460:463	the acetic acid	449:463	the acetic acid	449:463	Besides water, the acetic acid, with a maximum yield of 101.90gL-1 was the dominant compound in the aqueous fraction of liquid products.
28254732	4	9	theme	dominant	509:516	arg1	compound					518:525	the dominant compound	505:525	the dominant compound in the aqueous fraction of liquid products	505:568	Besides water, the acetic acid, with a maximum yield of 101.90gL-1 was the dominant compound in the aqueous fraction of liquid products.
28254732	2	10	theme	%	285:285	arg1	%					292:292	43% to 65%	283:292	43% to 65% for sweet sorghum bagasse	283:318	The torrefied solid yield ranged from 43% to 65% for sweet sorghum bagasse and 51-70% for energy sorghum.
28254732	2	11	theme	solid	259:263	arg1	yield					265:269	The torrefied solid yield	245:269	The torrefied solid yield	245:269	The torrefied solid yield ranged from 43% to 65% for sweet sorghum bagasse and 51-70% for energy sorghum.
28254732	2	12	theme	torrefied	249:257	arg1	yield					265:269	The torrefied solid yield	245:269	The torrefied solid yield	245:269	The torrefied solid yield ranged from 43% to 65% for sweet sorghum bagasse and 51-70% for energy sorghum.
28254732	2	13	theme	energy	335:340	arg1	sorghum					342:348	energy sorghum	335:348	energy sorghum	335:348	The torrefied solid yield ranged from 43% to 65% for sweet sorghum bagasse and 51-70% for energy sorghum.
28254732	6	14	from	compounds	814:822	arg1	fraction					835:842	the oil fraction	827:842	the oil fraction of the liquid product	827:864	Phenolic type chemicals and furan derivatives were the major compounds in the oil fraction of the liquid product, accounted up to 58wt%.
28254732	6	15	theme	major	808:812	arg1	compounds					814:822	the major compounds	804:822	the major compounds	804:822	Phenolic type chemicals and furan derivatives were the major compounds in the oil fraction of the liquid product, accounted up to 58wt%.
28254732	6	15	theme	major	808:812	arg1	chemicals					767:775	Phenolic type chemicals	753:775	Phenolic type chemicals	753:775	Phenolic type chemicals and furan derivatives were the major compounds in the oil fraction of the liquid product, accounted up to 58wt%.
28254732	6	15	theme	major	808:812	arg1	derivatives					787:797	furan derivatives	781:797	furan derivatives	781:797	Phenolic type chemicals and furan derivatives were the major compounds in the oil fraction of the liquid product, accounted up to 58wt%.
28254732	1	16	theme	energy	76:81	arg1	sorghum					83:89	energy sorghum and sweet sorghum bagasse	76:115	sorghum	83:89	Torrefaction of energy sorghum and sweet sorghum bagasse was investigated at three different temperatures (250, 275 & 300°C) for 30min to determine product yields and its compositions.
28254732	6	17	dep	%	887:887	arg1	up					877:878	up	877:878	up	877:878	Phenolic type chemicals and furan derivatives were the major compounds in the oil fraction of the liquid product, accounted up to 58wt%.
28254732	3	18	theme	torrefied	378:386	arg1	sorghums					388:395	both torrefied sorghums	373:395	both torrefied sorghums	373:395	The energy density of both torrefied sorghums increased between 1.6 and 1.4 folds.
28254732	1	19	theme	sorghum	83:89	arg1	Torrefaction					60:71	Torrefaction	60:71	Torrefaction of energy sorghum and sweet sorghum bagasse	60:115	Torrefaction of energy sorghum and sweet sorghum bagasse was investigated at three different temperatures (250, 275 & 300°C) for 30min to determine product yields and its compositions.
28254732	7	20	theme	high-value	951:960	arg1	chemicals					971:979	high-value platform chemicals	951:979	high-value platform chemicals	951:979	The condensable liquid products can be further upgraded into high-value platform chemicals.
28254732	5	21	theme	other	732:736	arg1	compounds					742:750	other key compounds	732:750	other key compounds	732:750	The aqueous fraction from sweet sorghum bagasse contained furfural and furan carboxyl aldehydes, while ketones and alcohols were dominant from energy sorghum as other key compounds.
28254732	4	22	theme	maximum	473:479	arg1	yield					481:485	a maximum yield	471:485	a maximum yield of 101.90gL-1	471:499	Besides water, the acetic acid, with a maximum yield of 101.90gL-1 was the dominant compound in the aqueous fraction of liquid products.
28254732	1	23	theme	&	176:176	arg1	temperatures					153:164	three different temperatures	137:164	three different temperatures (250, 275 & 300°C) for 30min to determine product yields	137:221	Torrefaction of energy sorghum and sweet sorghum bagasse was investigated at three different temperatures (250, 275 & 300°C) for 30min to determine product yields and its compositions.
28254732	1	23	theme	&	176:176	arg1	300°C					178:182	250, 275 & 300°C	167:182	300°C	178:182	Torrefaction of energy sorghum and sweet sorghum bagasse was investigated at three different temperatures (250, 275 & 300°C) for 30min to determine product yields and its compositions.
28254732	0	24	theme	biomass	24:30	arg1	Torrefaction					0:11	Torrefaction	0:11	Torrefaction of sorghum biomass	0:30	Torrefaction of sorghum biomass to improve fuel properties.
28254732	1	25	theme	sweet	95:99	arg1	bagasse					109:115	energy sorghum and sweet sorghum bagasse	76:115	bagasse	109:115	Torrefaction of energy sorghum and sweet sorghum bagasse was investigated at three different temperatures (250, 275 & 300°C) for 30min to determine product yields and its compositions.
28254732	0	26	theme	sorghum	16:22	arg1	biomass					24:30	sorghum biomass	16:30	sorghum biomass	16:30	Torrefaction of sorghum biomass to improve fuel properties.
28254732	5	27	theme	furfural	629:636	arg1	aldehydes					657:665	furfural and furan carboxyl aldehydes	629:665	furfural and furan carboxyl aldehydes	629:665	The aqueous fraction from sweet sorghum bagasse contained furfural and furan carboxyl aldehydes, while ketones and alcohols were dominant from energy sorghum as other key compounds.
28254732	1	28	theme	sorghum	101:107	arg1	bagasse					109:115	energy sorghum and sweet sorghum bagasse	76:115	bagasse	109:115	Torrefaction of energy sorghum and sweet sorghum bagasse was investigated at three different temperatures (250, 275 & 300°C) for 30min to determine product yields and its compositions.
28254732	5	29	theme	key	738:740	arg1	compounds					742:750	other key compounds	732:750	other key compounds	732:750	The aqueous fraction from sweet sorghum bagasse contained furfural and furan carboxyl aldehydes, while ketones and alcohols were dominant from energy sorghum as other key compounds.
28254732	5	30	contain	contained	619:627	arg2	aldehydes					657:665	furfural and furan carboxyl aldehydes	629:665	furfural and furan carboxyl aldehydes	629:665	The aqueous fraction from sweet sorghum bagasse contained furfural and furan carboxyl aldehydes, while ketones and alcohols were dominant from energy sorghum as other key compounds.
28254732	5	30	contain	contained	619:627	arg1	fraction					583:590	The aqueous fraction	571:590	The aqueous fraction from sweet sorghum bagasse	571:617	The aqueous fraction from sweet sorghum bagasse contained furfural and furan carboxyl aldehydes, while ketones and alcohols were dominant from energy sorghum as other key compounds.
28254732	7	31	theme	platform	962:969	arg1	chemicals					971:979	high-value platform chemicals	951:979	high-value platform chemicals	951:979	The condensable liquid products can be further upgraded into high-value platform chemicals.
28254732	4	32	theme	products	561:568	arg1	fraction					542:549	the aqueous fraction	530:549	the aqueous fraction of liquid products	530:568	Besides water, the acetic acid, with a maximum yield of 101.90gL-1 was the dominant compound in the aqueous fraction of liquid products.
28254732	3	33	theme	sorghums	388:395	arg1	density					362:368	The energy density	351:368	The energy density of both torrefied sorghums	351:395	The energy density of both torrefied sorghums increased between 1.6 and 1.4 folds.
28254732	3	34	theme	energy	355:360	arg1	density					362:368	The energy density	351:368	The energy density of both torrefied sorghums	351:395	The energy density of both torrefied sorghums increased between 1.6 and 1.4 folds.
28254732	5	35	theme	furan	642:646	arg1	aldehydes					657:665	furfural and furan carboxyl aldehydes	629:665	furfural and furan carboxyl aldehydes	629:665	The aqueous fraction from sweet sorghum bagasse contained furfural and furan carboxyl aldehydes, while ketones and alcohols were dominant from energy sorghum as other key compounds.
28254732	6	36	theme	furan	781:785	arg1	compounds					814:822	the major compounds	804:822	the major compounds	804:822	Phenolic type chemicals and furan derivatives were the major compounds in the oil fraction of the liquid product, accounted up to 58wt%.
28254732	6	36	theme	furan	781:785	arg1	chemicals					767:775	Phenolic type chemicals	753:775	Phenolic type chemicals	753:775	Phenolic type chemicals and furan derivatives were the major compounds in the oil fraction of the liquid product, accounted up to 58wt%.
28254732	6	36	theme	furan	781:785	arg1	derivatives					787:797	furan derivatives	781:797	furan derivatives	781:797	Phenolic type chemicals and furan derivatives were the major compounds in the oil fraction of the liquid product, accounted up to 58wt%.
28254732	4	37	theme	aqueous	534:540	arg1	fraction					542:549	the aqueous fraction	530:549	the aqueous fraction of liquid products	530:568	Besides water, the acetic acid, with a maximum yield of 101.90gL-1 was the dominant compound in the aqueous fraction of liquid products.
28254732	4	38	theme	liquid	554:559	arg1	products					561:568	liquid products	554:568	liquid products	554:568	Besides water, the acetic acid, with a maximum yield of 101.90gL-1 was the dominant compound in the aqueous fraction of liquid products.
28254732	5	39	theme	energy	714:719	arg1	sorghum					721:727	energy sorghum	714:727	energy sorghum as other key compounds	714:750	The aqueous fraction from sweet sorghum bagasse contained furfural and furan carboxyl aldehydes, while ketones and alcohols were dominant from energy sorghum as other key compounds.
28254732	4	40	theme	acetic	453:458	arg1	acid					460:463	the acetic acid	449:463	the acetic acid	449:463	Besides water, the acetic acid, with a maximum yield of 101.90gL-1 was the dominant compound in the aqueous fraction of liquid products.
28254732	4	40	theme	acetic	453:458	arg1	compound					518:525	the dominant compound	505:525	the dominant compound in the aqueous fraction of liquid products	505:568	Besides water, the acetic acid, with a maximum yield of 101.90gL-1 was the dominant compound in the aqueous fraction of liquid products.
28254732	1	41	theme	bagasse	109:115	arg1	Torrefaction					60:71	Torrefaction	60:71	Torrefaction of energy sorghum and sweet sorghum bagasse	60:115	Torrefaction of energy sorghum and sweet sorghum bagasse was investigated at three different temperatures (250, 275 & 300°C) for 30min to determine product yields and its compositions.
28254732	0	42	theme	fuel	43:46	arg1	properties					48:57	fuel properties	43:57	fuel properties	43:57	Torrefaction of sorghum biomass to improve fuel properties.
28254732	6	43	theme	liquid	851:856	arg1	product					858:864	the liquid product	847:864	the liquid product	847:864	Phenolic type chemicals and furan derivatives were the major compounds in the oil fraction of the liquid product, accounted up to 58wt%.
28254732	5	44	theme	sweet	597:601	arg1	bagasse					611:617	sweet sorghum bagasse	597:617	sweet sorghum bagasse	597:617	The aqueous fraction from sweet sorghum bagasse contained furfural and furan carboxyl aldehydes, while ketones and alcohols were dominant from energy sorghum as other key compounds.
28254732	7	45	theme	liquid	906:911	arg1	products					913:920	The condensable liquid products	890:920	The condensable liquid products	890:920	The condensable liquid products can be further upgraded into high-value platform chemicals.
28254732	5	46	theme	sorghum	603:609	arg1	bagasse					611:617	sweet sorghum bagasse	597:617	sweet sorghum bagasse	597:617	The aqueous fraction from sweet sorghum bagasse contained furfural and furan carboxyl aldehydes, while ketones and alcohols were dominant from energy sorghum as other key compounds.
28254732	6	47	theme	type	762:765	arg1	compounds					814:822	the major compounds	804:822	the major compounds	804:822	Phenolic type chemicals and furan derivatives were the major compounds in the oil fraction of the liquid product, accounted up to 58wt%.
28254732	6	47	theme	type	762:765	arg1	chemicals					767:775	Phenolic type chemicals	753:775	Phenolic type chemicals	753:775	Phenolic type chemicals and furan derivatives were the major compounds in the oil fraction of the liquid product, accounted up to 58wt%.
28254732	6	47	theme	type	762:765	arg1	derivatives					787:797	furan derivatives	781:797	furan derivatives	781:797	Phenolic type chemicals and furan derivatives were the major compounds in the oil fraction of the liquid product, accounted up to 58wt%.
28254732	4	48	with	acid	460:463	arg1	yield					481:485	a maximum yield	471:485	a maximum yield of 101.90gL-1	471:499	Besides water, the acetic acid, with a maximum yield of 101.90gL-1 was the dominant compound in the aqueous fraction of liquid products.
28254732	6	49	theme	Phenolic	753:760	arg1	compounds					814:822	the major compounds	804:822	the major compounds	804:822	Phenolic type chemicals and furan derivatives were the major compounds in the oil fraction of the liquid product, accounted up to 58wt%.
28254732	6	49	theme	Phenolic	753:760	arg1	chemicals					767:775	Phenolic type chemicals	753:775	Phenolic type chemicals	753:775	Phenolic type chemicals and furan derivatives were the major compounds in the oil fraction of the liquid product, accounted up to 58wt%.
28254732	6	49	theme	Phenolic	753:760	arg1	derivatives					787:797	furan derivatives	781:797	furan derivatives	781:797	Phenolic type chemicals and furan derivatives were the major compounds in the oil fraction of the liquid product, accounted up to 58wt%.
28254732	5	50	from	bagasse	611:617	arg1	fraction					583:590	The aqueous fraction	571:590	The aqueous fraction from sweet sorghum bagasse	571:617	The aqueous fraction from sweet sorghum bagasse contained furfural and furan carboxyl aldehydes, while ketones and alcohols were dominant from energy sorghum as other key compounds.
28254732	7	51	theme	condensable	894:904	arg1	products					913:920	The condensable liquid products	890:920	The condensable liquid products	890:920	The condensable liquid products can be further upgraded into high-value platform chemicals.
28254732	1	52	theme	product	208:214	arg1	yields					216:221	product yields	208:221	product yields	208:221	Torrefaction of energy sorghum and sweet sorghum bagasse was investigated at three different temperatures (250, 275 & 300°C) for 30min to determine product yields and its compositions.
28254732	3	53	dep	1.6	415:417	arg1	folds					427:431	folds	427:431	folds	427:431	The energy density of both torrefied sorghums increased between 1.6 and 1.4 folds.
28254732	1	54	theme	different	143:151	arg1	temperatures					153:164	three different temperatures	137:164	three different temperatures (250, 275 & 300°C) for 30min to determine product yields	137:221	Torrefaction of energy sorghum and sweet sorghum bagasse was investigated at three different temperatures (250, 275 & 300°C) for 30min to determine product yields and its compositions.
28254732	1	54	theme	different	143:151	arg1	300°C					178:182	250, 275 & 300°C	167:182	300°C	178:182	Torrefaction of energy sorghum and sweet sorghum bagasse was investigated at three different temperatures (250, 275 & 300°C) for 30min to determine product yields and its compositions.
28254732	6	55	theme	product	858:864	arg1	fraction					835:842	the oil fraction	827:842	the oil fraction of the liquid product	827:864	Phenolic type chemicals and furan derivatives were the major compounds in the oil fraction of the liquid product, accounted up to 58wt%.
27083352	0	0	from	Achillea	31:38	arg1	fraction					17:24	A polysaccharide fraction	0:24	A polysaccharide fraction from Achillea millefolium	0:50	A polysaccharide fraction from Achillea millefolium increases cytokine secretion and reduces activation of Akt, ERK and NF-κB in THP-1 monocytes.
27083352	1	1	theme	Achillea	146:153	arg1	millefolium					155:165	Achillea millefolium	146:165	Achillea millefolium	146:165	Achillea millefolium has been used in traditional medicine for a number of ailments, including skin inflammation and wounds.
27083352	5	2	theme	THP-1	805:809	arg1	monocytes					811:819	Stimulating THP-1 monocytes	793:819	Stimulating THP-1 monocytes in the presence of Am-25-d	793:846	Stimulating THP-1 monocytes in the presence of Am-25-d led to decreased nuclear concentrations of NF-κB and phosphorylation of ERK1/2 and Akt kinases compared with that when the cells were stimulated without Am-25-d.
27083352	5	3	dep	led	848:850	arg1	compared					943:950	compared	943:950	compared with that when the cells were stimulated without Am-25-d	943:1007	Stimulating THP-1 monocytes in the presence of Am-25-d led to decreased nuclear concentrations of NF-κB and phosphorylation of ERK1/2 and Akt kinases compared with that when the cells were stimulated without Am-25-d.
27083352	1	4	theme	skin	241:244	arg1	inflammation					246:257	skin inflammation	241:257	skin inflammation	241:257	Achillea millefolium has been used in traditional medicine for a number of ailments, including skin inflammation and wounds.
27083352	0	5	theme	Akt	107:109	arg1	activation					93:102	activation	93:102	activation of Akt, ERK and NF-κB in THP-1 monocytes	93:143	A polysaccharide fraction from Achillea millefolium increases cytokine secretion and reduces activation of Akt, ERK and NF-κB in THP-1 monocytes.
27083352	5	6	theme	phosphorylation	901:915	arg1	concentrations					873:886	decreased nuclear concentrations	855:886	decreased nuclear concentrations of NF-κB and phosphorylation of ERK1/2 and Akt kinases	855:941	Stimulating THP-1 monocytes in the presence of Am-25-d led to decreased nuclear concentrations of NF-κB and phosphorylation of ERK1/2 and Akt kinases compared with that when the cells were stimulated without Am-25-d.
27083352	5	7	from	monocytes	811:819	arg1	presence					828:835	the presence	824:835	the presence of Am-25-d	824:846	Stimulating THP-1 monocytes in the presence of Am-25-d led to decreased nuclear concentrations of NF-κB and phosphorylation of ERK1/2 and Akt kinases compared with that when the cells were stimulated without Am-25-d.
27083352	2	8	theme	polysaccharide	273:286	arg1	Am-25-d					298:304	Am-25-d	298:304	Am-25-d	298:304	A polysaccharide fraction (Am-25-d) isolated from aqueous extract from A. millefolium had an average molecular weight of 270 kDa and a monosaccharide composition of GalA, Gal, Ara, Xyl, Rha in molar ratio of 28:26:23:9:7.
27083352	2	8	theme	polysaccharide	273:286	arg1	fraction					288:295	A polysaccharide fraction	271:295	A polysaccharide fraction (Am-25-d) isolated from aqueous extract from A. millefolium	271:355	A polysaccharide fraction (Am-25-d) isolated from aqueous extract from A. millefolium had an average molecular weight of 270 kDa and a monosaccharide composition of GalA, Gal, Ara, Xyl, Rha in molar ratio of 28:26:23:9:7.
27083352	2	9	theme	molar	464:468	arg1	ratio					470:474	molar ratio	464:474	molar ratio of 28:26:23:9:7	464:490	A polysaccharide fraction (Am-25-d) isolated from aqueous extract from A. millefolium had an average molecular weight of 270 kDa and a monosaccharide composition of GalA, Gal, Ara, Xyl, Rha in molar ratio of 28:26:23:9:7.
27083352	4	10	theme	cytokines	773:781	arg1	secretion					756:764	secretion	756:764	secretion of the cytokines examined	756:790	However, when added to unstimulated cells Am-25-d did not increase secretion of the cytokines examined.
27083352	3	11	theme	THP-1	493:497	arg1	cells					499:503	THP-1 cells	493:503	THP-1 cells primed with IFN-γ and stimulated with LPS in the presence of Am-25-d	493:572	THP-1 cells primed with IFN-γ and stimulated with LPS in the presence of Am-25-d secreted more IL-1β, IL-8, IL-10, IL-12p40, IL-23 and TNF-α than THP-1 cells stimulated in the absence of Am-25-d.
27083352	6	12	contain	has	1076:1078	arg2	properties					1096:1105	immunoenhancing properties	1080:1105	immunoenhancing properties that may be mediated via the Akt pathway	1080:1146	These findings indicate that Am-25-d isolated from A. millefolium has immunoenhancing properties that may be mediated via the Akt pathway.
27083352	6	12	contain	has	1076:1078	arg1	Am-25-d					1039:1045	Am-25-d	1039:1045	Am-25-d isolated from A. millefolium	1039:1074	These findings indicate that Am-25-d isolated from A. millefolium has immunoenhancing properties that may be mediated via the Akt pathway.
27083352	0	13	theme	ERK	112:114	arg1	activation					93:102	activation	93:102	activation of Akt, ERK and NF-κB in THP-1 monocytes	93:143	A polysaccharide fraction from Achillea millefolium increases cytokine secretion and reduces activation of Akt, ERK and NF-κB in THP-1 monocytes.
27083352	3	14	theme	THP-1	639:643	arg1	cells					645:649	THP-1 cells	639:649	THP-1 cells stimulated in the absence of Am-25-d	639:686	THP-1 cells primed with IFN-γ and stimulated with LPS in the presence of Am-25-d secreted more IL-1β, IL-8, IL-10, IL-12p40, IL-23 and TNF-α than THP-1 cells stimulated in the absence of Am-25-d.
27083352	5	15	dep	ERK1/2	920:925	arg1	kinases					935:941	kinases	935:941	kinases	935:941	Stimulating THP-1 monocytes in the presence of Am-25-d led to decreased nuclear concentrations of NF-κB and phosphorylation of ERK1/2 and Akt kinases compared with that when the cells were stimulated without Am-25-d.
27083352	2	16	attach	isolated	307:314	arg1	extract					329:335	aqueous extract	321:335	aqueous extract from A. millefolium	321:355	A polysaccharide fraction (Am-25-d) isolated from aqueous extract from A. millefolium had an average molecular weight of 270 kDa and a monosaccharide composition of GalA, Gal, Ara, Xyl, Rha in molar ratio of 28:26:23:9:7.
27083352	2	16	attach	isolated	307:314	arg2	Am-25-d					298:304	Am-25-d	298:304	Am-25-d	298:304	A polysaccharide fraction (Am-25-d) isolated from aqueous extract from A. millefolium had an average molecular weight of 270 kDa and a monosaccharide composition of GalA, Gal, Ara, Xyl, Rha in molar ratio of 28:26:23:9:7.
27083352	2	16	attach	isolated	307:314	arg2	fraction					288:295	A polysaccharide fraction	271:295	A polysaccharide fraction (Am-25-d) isolated from aqueous extract from A. millefolium	271:355	A polysaccharide fraction (Am-25-d) isolated from aqueous extract from A. millefolium had an average molecular weight of 270 kDa and a monosaccharide composition of GalA, Gal, Ara, Xyl, Rha in molar ratio of 28:26:23:9:7.
27083352	5	17	theme	NF-κB	891:895	arg1	concentrations					873:886	decreased nuclear concentrations	855:886	decreased nuclear concentrations of NF-κB and phosphorylation of ERK1/2 and Akt kinases	855:941	Stimulating THP-1 monocytes in the presence of Am-25-d led to decreased nuclear concentrations of NF-κB and phosphorylation of ERK1/2 and Akt kinases compared with that when the cells were stimulated without Am-25-d.
27083352	2	18	theme	molecular	372:380	arg1	weight					382:387	an average molecular weight	361:387	an average molecular weight of 270 kDa	361:398	A polysaccharide fraction (Am-25-d) isolated from aqueous extract from A. millefolium had an average molecular weight of 270 kDa and a monosaccharide composition of GalA, Gal, Ara, Xyl, Rha in molar ratio of 28:26:23:9:7.
27083352	0	19	theme	polysaccharide	2:15	arg1	fraction					17:24	A polysaccharide fraction	0:24	A polysaccharide fraction from Achillea millefolium	0:50	A polysaccharide fraction from Achillea millefolium increases cytokine secretion and reduces activation of Akt, ERK and NF-κB in THP-1 monocytes.
27083352	2	20	contain	had	357:359	arg1	Am-25-d					298:304	Am-25-d	298:304	Am-25-d	298:304	A polysaccharide fraction (Am-25-d) isolated from aqueous extract from A. millefolium had an average molecular weight of 270 kDa and a monosaccharide composition of GalA, Gal, Ara, Xyl, Rha in molar ratio of 28:26:23:9:7.
27083352	2	20	contain	had	357:359	arg2	weight					382:387	an average molecular weight	361:387	an average molecular weight of 270 kDa	361:398	A polysaccharide fraction (Am-25-d) isolated from aqueous extract from A. millefolium had an average molecular weight of 270 kDa and a monosaccharide composition of GalA, Gal, Ara, Xyl, Rha in molar ratio of 28:26:23:9:7.
27083352	2	20	contain	had	357:359	arg1	fraction					288:295	A polysaccharide fraction	271:295	A polysaccharide fraction (Am-25-d) isolated from aqueous extract from A. millefolium	271:355	A polysaccharide fraction (Am-25-d) isolated from aqueous extract from A. millefolium had an average molecular weight of 270 kDa and a monosaccharide composition of GalA, Gal, Ara, Xyl, Rha in molar ratio of 28:26:23:9:7.
27083352	2	20	contain	had	357:359	arg2	composition					421:431	a monosaccharide composition	404:431	a monosaccharide composition of GalA, Gal, Ara, Xyl, Rha	404:459	A polysaccharide fraction (Am-25-d) isolated from aqueous extract from A. millefolium had an average molecular weight of 270 kDa and a monosaccharide composition of GalA, Gal, Ara, Xyl, Rha in molar ratio of 28:26:23:9:7.
27083352	5	21	theme	Am-25-d	840:846	arg1	presence					828:835	the presence	824:835	the presence of Am-25-d	824:846	Stimulating THP-1 monocytes in the presence of Am-25-d led to decreased nuclear concentrations of NF-κB and phosphorylation of ERK1/2 and Akt kinases compared with that when the cells were stimulated without Am-25-d.
27083352	2	22	theme	average	364:370	arg1	weight					382:387	an average molecular weight	361:387	an average molecular weight of 270 kDa	361:398	A polysaccharide fraction (Am-25-d) isolated from aqueous extract from A. millefolium had an average molecular weight of 270 kDa and a monosaccharide composition of GalA, Gal, Ara, Xyl, Rha in molar ratio of 28:26:23:9:7.
27083352	0	23	theme	THP-1	129:133	arg1	monocytes					135:143	THP-1 monocytes	129:143	THP-1 monocytes	129:143	A polysaccharide fraction from Achillea millefolium increases cytokine secretion and reduces activation of Akt, ERK and NF-κB in THP-1 monocytes.
27083352	2	24	theme	monosaccharide	406:419	arg1	composition					421:431	a monosaccharide composition	404:431	a monosaccharide composition of GalA, Gal, Ara, Xyl, Rha	404:459	A polysaccharide fraction (Am-25-d) isolated from aqueous extract from A. millefolium had an average molecular weight of 270 kDa and a monosaccharide composition of GalA, Gal, Ara, Xyl, Rha in molar ratio of 28:26:23:9:7.
27083352	0	25	theme	NF-κB	120:124	arg1	activation					93:102	activation	93:102	activation of Akt, ERK and NF-κB in THP-1 monocytes	93:143	A polysaccharide fraction from Achillea millefolium increases cytokine secretion and reduces activation of Akt, ERK and NF-κB in THP-1 monocytes.
27083352	2	26	from	millefolium	345:355	arg1	extract					329:335	aqueous extract	321:335	aqueous extract from A. millefolium	321:355	A polysaccharide fraction (Am-25-d) isolated from aqueous extract from A. millefolium had an average molecular weight of 270 kDa and a monosaccharide composition of GalA, Gal, Ara, Xyl, Rha in molar ratio of 28:26:23:9:7.
27083352	1	27	theme	traditional	184:194	arg1	medicine					196:203	traditional medicine	184:203	traditional medicine	184:203	Achillea millefolium has been used in traditional medicine for a number of ailments, including skin inflammation and wounds.
27083352	0	28	from	activation	93:102	arg1	monocytes					135:143	THP-1 monocytes	129:143	THP-1 monocytes	129:143	A polysaccharide fraction from Achillea millefolium increases cytokine secretion and reduces activation of Akt, ERK and NF-κB in THP-1 monocytes.
27083352	5	29	theme	decreased	855:863	arg1	concentrations					873:886	decreased nuclear concentrations	855:886	decreased nuclear concentrations of NF-κB and phosphorylation of ERK1/2 and Akt kinases	855:941	Stimulating THP-1 monocytes in the presence of Am-25-d led to decreased nuclear concentrations of NF-κB and phosphorylation of ERK1/2 and Akt kinases compared with that when the cells were stimulated without Am-25-d.
27083352	2	30	theme	kDa	396:398	arg1	composition					421:431	a monosaccharide composition	404:431	a monosaccharide composition of GalA, Gal, Ara, Xyl, Rha	404:459	A polysaccharide fraction (Am-25-d) isolated from aqueous extract from A. millefolium had an average molecular weight of 270 kDa and a monosaccharide composition of GalA, Gal, Ara, Xyl, Rha in molar ratio of 28:26:23:9:7.
27083352	2	30	theme	kDa	396:398	arg1	weight					382:387	an average molecular weight	361:387	an average molecular weight of 270 kDa	361:398	A polysaccharide fraction (Am-25-d) isolated from aqueous extract from A. millefolium had an average molecular weight of 270 kDa and a monosaccharide composition of GalA, Gal, Ara, Xyl, Rha in molar ratio of 28:26:23:9:7.
27083352	5	31	theme	nuclear	865:871	arg1	concentrations					873:886	decreased nuclear concentrations	855:886	decreased nuclear concentrations of NF-κB and phosphorylation of ERK1/2 and Akt kinases	855:941	Stimulating THP-1 monocytes in the presence of Am-25-d led to decreased nuclear concentrations of NF-κB and phosphorylation of ERK1/2 and Akt kinases compared with that when the cells were stimulated without Am-25-d.
27083352	2	32	theme	28:26:23:9:7	479:490	arg1	ratio					470:474	molar ratio	464:474	molar ratio of 28:26:23:9:7	464:490	A polysaccharide fraction (Am-25-d) isolated from aqueous extract from A. millefolium had an average molecular weight of 270 kDa and a monosaccharide composition of GalA, Gal, Ara, Xyl, Rha in molar ratio of 28:26:23:9:7.
27083352	4	33	theme	unstimulated	712:723	arg1	cells					725:729	unstimulated cells	712:729	unstimulated cells	712:729	However, when added to unstimulated cells Am-25-d did not increase secretion of the cytokines examined.
27083352	3	34	from	LPS	543:545	arg1	presence					554:561	the presence	550:561	the presence of Am-25-d	550:572	THP-1 cells primed with IFN-γ and stimulated with LPS in the presence of Am-25-d secreted more IL-1β, IL-8, IL-10, IL-12p40, IL-23 and TNF-α than THP-1 cells stimulated in the absence of Am-25-d.
27083352	3	35	theme	Am-25-d	680:686	arg1	absence					669:675	the absence	665:675	the absence of Am-25-d	665:686	THP-1 cells primed with IFN-γ and stimulated with LPS in the presence of Am-25-d secreted more IL-1β, IL-8, IL-10, IL-12p40, IL-23 and TNF-α than THP-1 cells stimulated in the absence of Am-25-d.
27083352	3	36	theme	Am-25-d	566:572	arg1	presence					554:561	the presence	550:561	the presence of Am-25-d	550:572	THP-1 cells primed with IFN-γ and stimulated with LPS in the presence of Am-25-d secreted more IL-1β, IL-8, IL-10, IL-12p40, IL-23 and TNF-α than THP-1 cells stimulated in the absence of Am-25-d.
27083352	5	37	theme	Akt	931:933	arg1	phosphorylation					901:915	phosphorylation	901:915	phosphorylation	901:915	Stimulating THP-1 monocytes in the presence of Am-25-d led to decreased nuclear concentrations of NF-κB and phosphorylation of ERK1/2 and Akt kinases compared with that when the cells were stimulated without Am-25-d.
27083352	5	37	theme	Akt	931:933	arg1	NF-κB					891:895	NF-κB	891:895	NF-κB	891:895	Stimulating THP-1 monocytes in the presence of Am-25-d led to decreased nuclear concentrations of NF-κB and phosphorylation of ERK1/2 and Akt kinases compared with that when the cells were stimulated without Am-25-d.
27083352	0	38	theme	cytokine	62:69	arg1	secretion					71:79	cytokine secretion	62:79	cytokine secretion	62:79	A polysaccharide fraction from Achillea millefolium increases cytokine secretion and reduces activation of Akt, ERK and NF-κB in THP-1 monocytes.
27083352	2	39	theme	GalA	436:439	arg1	composition					421:431	a monosaccharide composition	404:431	a monosaccharide composition of GalA, Gal, Ara, Xyl, Rha	404:459	A polysaccharide fraction (Am-25-d) isolated from aqueous extract from A. millefolium had an average molecular weight of 270 kDa and a monosaccharide composition of GalA, Gal, Ara, Xyl, Rha in molar ratio of 28:26:23:9:7.
27083352	2	39	theme	GalA	436:439	arg1	weight					382:387	an average molecular weight	361:387	an average molecular weight of 270 kDa	361:398	A polysaccharide fraction (Am-25-d) isolated from aqueous extract from A. millefolium had an average molecular weight of 270 kDa and a monosaccharide composition of GalA, Gal, Ara, Xyl, Rha in molar ratio of 28:26:23:9:7.
27083352	0	40	dep	Achillea	31:38	arg1	millefolium					40:50	Achillea millefolium	31:50	Achillea millefolium	31:50	A polysaccharide fraction from Achillea millefolium increases cytokine secretion and reduces activation of Akt, ERK and NF-κB in THP-1 monocytes.
27083352	2	41	theme	aqueous	321:327	arg1	extract					329:335	aqueous extract	321:335	aqueous extract from A. millefolium	321:355	A polysaccharide fraction (Am-25-d) isolated from aqueous extract from A. millefolium had an average molecular weight of 270 kDa and a monosaccharide composition of GalA, Gal, Ara, Xyl, Rha in molar ratio of 28:26:23:9:7.
27083352	6	42	theme	Akt	1136:1138	arg1	pathway					1140:1146	the Akt pathway	1132:1146	the Akt pathway	1132:1146	These findings indicate that Am-25-d isolated from A. millefolium has immunoenhancing properties that may be mediated via the Akt pathway.
27083352	6	43	attach	isolated	1047:1054	arg1	millefolium					1064:1074	A. millefolium	1061:1074	A. millefolium	1061:1074	These findings indicate that Am-25-d isolated from A. millefolium has immunoenhancing properties that may be mediated via the Akt pathway.
27083352	6	43	attach	isolated	1047:1054	arg2	Am-25-d					1039:1045	Am-25-d	1039:1045	Am-25-d isolated from A. millefolium	1039:1074	These findings indicate that Am-25-d isolated from A. millefolium has immunoenhancing properties that may be mediated via the Akt pathway.
27083352	6	44	theme	immunoenhancing	1080:1094	arg1	properties					1096:1105	immunoenhancing properties	1080:1105	immunoenhancing properties that may be mediated via the Akt pathway	1080:1146	These findings indicate that Am-25-d isolated from A. millefolium has immunoenhancing properties that may be mediated via the Akt pathway.
27083352	1	45	used	used	176:179	arg2	millefolium					155:165	Achillea millefolium	146:165	Achillea millefolium	146:165	Achillea millefolium has been used in traditional medicine for a number of ailments, including skin inflammation and wounds.
27083352	3	46	dep	cells	499:503	arg1	primed					505:510	primed	505:510	primed with IFN-γ	505:521	THP-1 cells primed with IFN-γ and stimulated with LPS in the presence of Am-25-d secreted more IL-1β, IL-8, IL-10, IL-12p40, IL-23 and TNF-α than THP-1 cells stimulated in the absence of Am-25-d.
27083352	3	46	dep	cells	499:503	arg1	stimulated					527:536	stimulated	527:536	stimulated with LPS in the presence of Am-25-d	527:572	THP-1 cells primed with IFN-γ and stimulated with LPS in the presence of Am-25-d secreted more IL-1β, IL-8, IL-10, IL-12p40, IL-23 and TNF-α than THP-1 cells stimulated in the absence of Am-25-d.
27083352	1	47	theme	ailments	221:228	arg1	number					211:216	a number	209:216	a number	209:216	Achillea millefolium has been used in traditional medicine for a number of ailments, including skin inflammation and wounds.
27083352	1	47	theme	ailments	221:228	arg1	inflammation					246:257	skin inflammation	241:257	skin inflammation	241:257	Achillea millefolium has been used in traditional medicine for a number of ailments, including skin inflammation and wounds.
27083352	1	47	theme	ailments	221:228	arg1	wounds					263:268	wounds	263:268	wounds	263:268	Achillea millefolium has been used in traditional medicine for a number of ailments, including skin inflammation and wounds.
27083352	5	48	theme	ERK1/2	920:925	arg1	phosphorylation					901:915	phosphorylation	901:915	phosphorylation	901:915	Stimulating THP-1 monocytes in the presence of Am-25-d led to decreased nuclear concentrations of NF-κB and phosphorylation of ERK1/2 and Akt kinases compared with that when the cells were stimulated without Am-25-d.
27083352	5	48	theme	ERK1/2	920:925	arg1	NF-κB					891:895	NF-κB	891:895	NF-κB	891:895	Stimulating THP-1 monocytes in the presence of Am-25-d led to decreased nuclear concentrations of NF-κB and phosphorylation of ERK1/2 and Akt kinases compared with that when the cells were stimulated without Am-25-d.
27083352	5	49	theme	Stimulating	793:803	arg1	monocytes					811:819	Stimulating THP-1 monocytes	793:819	Stimulating THP-1 monocytes in the presence of Am-25-d	793:846	Stimulating THP-1 monocytes in the presence of Am-25-d led to decreased nuclear concentrations of NF-κB and phosphorylation of ERK1/2 and Akt kinases compared with that when the cells were stimulated without Am-25-d.
26187191	4	0	theme	different	449:457	arg1	composites					459:468	Two different composites	445:468	Two different composites	445:468	Two different composites were prepared by incorporating 50% (w/w) HA/(β-TCP) in CHS or CHC followed by lyophilization and cross-linking of composites by tripolyphosphate (TPP).
26187191	7	1	theme	pore	955:958	arg1	Porosity					945:952	Porosity	945:952	Porosity	945:952	Porosity, pore size, water uptake; water retention abilities and in vitro degradations of the prepared composites were evaluated.
26187191	7	1	theme	pore	955:958	arg1	size					960:963	pore size	955:963	pore size	955:963	Porosity, pore size, water uptake; water retention abilities and in vitro degradations of the prepared composites were evaluated.
26187191	1	2	theme	chitosan/hydroxyapatite	130:152	arg1	composites					190:199	chitosan/hydroxyapatite (HA)/β-tircalcium phosphate (β-TCP) composites	130:199	chitosan/hydroxyapatite (HA)/β-tircalcium phosphate (β-TCP) composites	130:199	In the present study, chitosan/hydroxyapatite (HA)/β-tircalcium phosphate (β-TCP) composites were produced using squid pen derived chitosan (CHS) and commercial crab derived chitosan (CHC).
26187191	1	3	theme	pen	227:229	arg1	CHS					249:251	CHS	249:251	CHS	249:251	In the present study, chitosan/hydroxyapatite (HA)/β-tircalcium phosphate (β-TCP) composites were produced using squid pen derived chitosan (CHS) and commercial crab derived chitosan (CHC).
26187191	1	3	theme	pen	227:229	arg1	chitosan					239:246	squid pen derived chitosan	221:246	squid pen derived chitosan (CHS)	221:252	In the present study, chitosan/hydroxyapatite (HA)/β-tircalcium phosphate (β-TCP) composites were produced using squid pen derived chitosan (CHS) and commercial crab derived chitosan (CHC).
26187191	0	4	theme	crab	65:68	arg1	chitosan					70:77	crab chitosan	65:77	crab chitosan	65:77	Bio-mimetic composite scaffold from mussel shells, squid pen and crab chitosan for bone tissue engineering.
26187191	6	5	theme	preliminary	814:824	arg1	study					835:839	preliminary in vitro study	814:839	preliminary in vitro study	814:839	A simulated body fluid (SBF) solution was used for preliminary in vitro study for 1, 7, 14 and 28 days and the composites were characterized by XRD, FTIR, TGA, SEM, μ-CT and ICP-MS.
26187191	9	6	theme	bone	1438:1441	arg1	applications					1462:1473	bone tissue engineering applications	1438:1473	bone tissue engineering applications	1438:1473	The results of this study indicated that composites produced at -20 °C had higher mechanical properties and lower degradation rate compared with -80 °C. Chitosan from the squid pen is an excellent biomaterial candidate for bone tissue engineering applications.
26187191	6	7	theme	body	775:778	arg1	fluid					780:784	A simulated body fluid	763:784	A simulated body fluid (SBF) solution	763:799	A simulated body fluid (SBF) solution was used for preliminary in vitro study for 1, 7, 14 and 28 days and the composites were characterized by XRD, FTIR, TGA, SEM, μ-CT and ICP-MS.
26187191	6	7	theme	body	775:778	arg1	SBF					787:789	SBF	787:789	SBF	787:789	A simulated body fluid (SBF) solution was used for preliminary in vitro study for 1, 7, 14 and 28 days and the composites were characterized by XRD, FTIR, TGA, SEM, μ-CT and ICP-MS.
26187191	1	8	theme	derived	231:237	arg1	CHS					249:251	CHS	249:251	CHS	249:251	In the present study, chitosan/hydroxyapatite (HA)/β-tircalcium phosphate (β-TCP) composites were produced using squid pen derived chitosan (CHS) and commercial crab derived chitosan (CHC).
26187191	1	8	theme	derived	231:237	arg1	chitosan					239:246	squid pen derived chitosan	221:246	squid pen derived chitosan (CHS)	221:252	In the present study, chitosan/hydroxyapatite (HA)/β-tircalcium phosphate (β-TCP) composites were produced using squid pen derived chitosan (CHS) and commercial crab derived chitosan (CHC).
26187191	0	9	theme	bone	83:86	arg1	engineering					95:105	bone tissue engineering	83:105	bone tissue engineering	83:105	Bio-mimetic composite scaffold from mussel shells, squid pen and crab chitosan for bone tissue engineering.
26187191	7	10	theme	Porosity	945:952	arg1	abilities					996:1004	Porosity, pore size, water uptake; water retention abilities	945:1004	Porosity, pore size, water uptake; water retention abilities	945:1004	Porosity, pore size, water uptake; water retention abilities and in vitro degradations of the prepared composites were evaluated.
26187191	9	11	theme	degradation	1329:1339	arg1	rate					1341:1344	lower degradation rate	1323:1344	lower degradation rate	1323:1344	The results of this study indicated that composites produced at -20 °C had higher mechanical properties and lower degradation rate compared with -80 °C. Chitosan from the squid pen is an excellent biomaterial candidate for bone tissue engineering applications.
26187191	5	12	theme	temperatures	655:666	arg1	effect					626:631	The effect	622:631	The effect of different freezing temperatures of -20, -80 and -196 °C on the physicochemical characteristics of composites	622:743	The effect of different freezing temperatures of -20, -80 and -196 °C on the physicochemical characteristics of composites was investigated.
26187191	9	13	theme	higher	1290:1295	arg1	properties					1308:1317	higher mechanical properties	1290:1317	higher mechanical properties	1290:1317	The results of this study indicated that composites produced at -20 °C had higher mechanical properties and lower degradation rate compared with -80 °C. Chitosan from the squid pen is an excellent biomaterial candidate for bone tissue engineering applications.
26187191	9	14	theme	engineering	1450:1460	arg1	applications					1462:1473	bone tissue engineering applications	1438:1473	bone tissue engineering applications	1438:1473	The results of this study indicated that composites produced at -20 °C had higher mechanical properties and lower degradation rate compared with -80 °C. Chitosan from the squid pen is an excellent biomaterial candidate for bone tissue engineering applications.
26187191	5	15	from	effect	626:631	arg1	characteristics					715:729	the physicochemical characteristics	695:729	the physicochemical characteristics of composites	695:743	The effect of different freezing temperatures of -20, -80 and -196 °C on the physicochemical characteristics of composites was investigated.
26187191	7	16	theme	in	1010:1011	arg1	degradations					1019:1030	in vitro degradations	1010:1030	in vitro degradations	1010:1030	Porosity, pore size, water uptake; water retention abilities and in vitro degradations of the prepared composites were evaluated.
26187191	8	17	theme	higher	1113:1118	arg1	porosity					1120:1127	higher porosity	1113:1127	higher porosity (62%)	1113:1133	The CHS composites were found to have higher porosity (62%) compared to the CHC composites (porosity 42%) and better mechanical properties.
26187191	8	17	theme	higher	1113:1118	arg1	%					1132:1132	62%	1130:1132	62%	1130:1132	The CHS composites were found to have higher porosity (62%) compared to the CHC composites (porosity 42%) and better mechanical properties.
26187191	1	18	link	derived	231:237	arg1	CHS					249:251	CHS	249:251	CHS	249:251	In the present study, chitosan/hydroxyapatite (HA)/β-tircalcium phosphate (β-TCP) composites were produced using squid pen derived chitosan (CHS) and commercial crab derived chitosan (CHC).
26187191	1	18	link	derived	231:237	arg1	chitosan					239:246	squid pen derived chitosan	221:246	squid pen derived chitosan (CHS)	221:252	In the present study, chitosan/hydroxyapatite (HA)/β-tircalcium phosphate (β-TCP) composites were produced using squid pen derived chitosan (CHS) and commercial crab derived chitosan (CHC).
26187191	8	19	theme	CHS	1079:1081	arg1	composites					1083:1092	The CHS composites	1075:1092	The CHS composites	1075:1092	The CHS composites were found to have higher porosity (62%) compared to the CHC composites (porosity 42%) and better mechanical properties.
26187191	4	20	theme	composites	584:593	arg1	cross-linking					567:579	cross-linking	567:579	cross-linking of composites by tripolyphosphate (TPP)	567:619	Two different composites were prepared by incorporating 50% (w/w) HA/(β-TCP) in CHS or CHC followed by lyophilization and cross-linking of composites by tripolyphosphate (TPP).
26187191	4	20	theme	composites	584:593	arg1	lyophilization					548:561	lyophilization	548:561	lyophilization	548:561	Two different composites were prepared by incorporating 50% (w/w) HA/(β-TCP) in CHS or CHC followed by lyophilization and cross-linking of composites by tripolyphosphate (TPP).
26187191	0	21	theme	tissue	88:93	arg1	engineering					95:105	bone tissue engineering	83:105	bone tissue engineering	83:105	Bio-mimetic composite scaffold from mussel shells, squid pen and crab chitosan for bone tissue engineering.
26187191	0	22	from	shells	43:48	arg1	scaffold					22:29	Bio-mimetic composite scaffold	0:29	Bio-mimetic composite scaffold from mussel shells, squid pen and crab chitosan for bone tissue engineering.	0:106	Bio-mimetic composite scaffold from mussel shells, squid pen and crab chitosan for bone tissue engineering.
26187191	9	23	dep	pen	1392:1394	arg1	candidate					1424:1432	an excellent biomaterial candidate	1399:1432	an excellent biomaterial candidate for bone tissue engineering applications	1399:1473	The results of this study indicated that composites produced at -20 °C had higher mechanical properties and lower degradation rate compared with -80 °C. Chitosan from the squid pen is an excellent biomaterial candidate for bone tissue engineering applications.
26187191	8	24	theme	CHC	1151:1153	arg1	composites					1155:1164	the CHC composites	1147:1164	the CHC composites (porosity 42%)	1147:1179	The CHS composites were found to have higher porosity (62%) compared to the CHC composites (porosity 42%) and better mechanical properties.
26187191	8	24	theme	CHC	1151:1153	arg1	%					1178:1178	porosity 42%	1167:1178	porosity 42%	1167:1178	The CHS composites were found to have higher porosity (62%) compared to the CHC composites (porosity 42%) and better mechanical properties.
26187191	2	25	theme	alkaline	334:341	arg1	N-deacetylation					343:357	alkaline N-deacetylation	334:357	alkaline N-deacetylation	334:357	CHS was prepared from squid pens by alkaline N-deacetylation.
26187191	0	26	theme	composite	12:20	arg1	scaffold					22:29	Bio-mimetic composite scaffold	0:29	Bio-mimetic composite scaffold from mussel shells, squid pen and crab chitosan for bone tissue engineering.	0:106	Bio-mimetic composite scaffold from mussel shells, squid pen and crab chitosan for bone tissue engineering.
26187191	6	27	used	used	805:808	arg2	solution					792:799	A simulated body fluid (SBF) solution	763:799	A simulated body fluid (SBF) solution	763:799	A simulated body fluid (SBF) solution was used for preliminary in vitro study for 1, 7, 14 and 28 days and the composites were characterized by XRD, FTIR, TGA, SEM, μ-CT and ICP-MS.
26187191	7	28	theme	prepared	1039:1046	arg1	composites					1048:1057	the prepared composites	1035:1057	the prepared composites	1035:1057	Porosity, pore size, water uptake; water retention abilities and in vitro degradations of the prepared composites were evaluated.
26187191	8	29	theme	porosity	1167:1174	arg1	composites					1155:1164	the CHC composites	1147:1164	the CHC composites (porosity 42%)	1147:1179	The CHS composites were found to have higher porosity (62%) compared to the CHC composites (porosity 42%) and better mechanical properties.
26187191	8	29	theme	porosity	1167:1174	arg1	%					1178:1178	porosity 42%	1167:1178	porosity 42%	1167:1178	The CHS composites were found to have higher porosity (62%) compared to the CHC composites (porosity 42%) and better mechanical properties.
26187191	1	30	theme	/β-tircalcium	158:170	arg1	composites					190:199	chitosan/hydroxyapatite (HA)/β-tircalcium phosphate (β-TCP) composites	130:199	chitosan/hydroxyapatite (HA)/β-tircalcium phosphate (β-TCP) composites	130:199	In the present study, chitosan/hydroxyapatite (HA)/β-tircalcium phosphate (β-TCP) composites were produced using squid pen derived chitosan (CHS) and commercial crab derived chitosan (CHC).
26187191	0	31	theme	Bio-mimetic	0:10	arg1	scaffold					22:29	Bio-mimetic composite scaffold	0:29	Bio-mimetic composite scaffold from mussel shells, squid pen and crab chitosan for bone tissue engineering.	0:106	Bio-mimetic composite scaffold from mussel shells, squid pen and crab chitosan for bone tissue engineering.
26187191	6	32	dep	in	826:827	arg1	vitro					829:833	vitro	829:833	vitro	829:833	A simulated body fluid (SBF) solution was used for preliminary in vitro study for 1, 7, 14 and 28 days and the composites were characterized by XRD, FTIR, TGA, SEM, μ-CT and ICP-MS.
26187191	9	33	theme	°C.	1364:1366	arg1	Chitosan					1368:1375	-80 °C. Chitosan	1360:1375	-80 °C. Chitosan from the squid pen is an excellent biomaterial candidate for bone tissue engineering applications	1360:1473	The results of this study indicated that composites produced at -20 °C had higher mechanical properties and lower degradation rate compared with -80 °C. Chitosan from the squid pen is an excellent biomaterial candidate for bone tissue engineering applications.
26187191	5	34	theme	freezing	646:653	arg1	temperatures					655:666	different freezing temperatures	636:666	different freezing temperatures of -20, -80 and -196 °C	636:690	The effect of different freezing temperatures of -20, -80 and -196 °C on the physicochemical characteristics of composites was investigated.
26187191	1	35	theme	phosphate	172:180	arg1	composites					190:199	chitosan/hydroxyapatite (HA)/β-tircalcium phosphate (β-TCP) composites	130:199	chitosan/hydroxyapatite (HA)/β-tircalcium phosphate (β-TCP) composites	130:199	In the present study, chitosan/hydroxyapatite (HA)/β-tircalcium phosphate (β-TCP) composites were produced using squid pen derived chitosan (CHS) and commercial crab derived chitosan (CHC).
26187191	5	36	theme	composites	734:743	arg1	characteristics					715:729	the physicochemical characteristics	695:729	the physicochemical characteristics of composites	695:743	The effect of different freezing temperatures of -20, -80 and -196 °C on the physicochemical characteristics of composites was investigated.
26187191	9	37	from	pen	1392:1394	arg1	Chitosan					1368:1375	-80 °C. Chitosan	1360:1375	-80 °C. Chitosan from the squid pen is an excellent biomaterial candidate for bone tissue engineering applications	1360:1473	The results of this study indicated that composites produced at -20 °C had higher mechanical properties and lower degradation rate compared with -80 °C. Chitosan from the squid pen is an excellent biomaterial candidate for bone tissue engineering applications.
26187191	9	38	contain	had	1286:1288	arg1	composites					1256:1265	composites	1256:1265	composites produced at -20 °C	1256:1284	The results of this study indicated that composites produced at -20 °C had higher mechanical properties and lower degradation rate compared with -80 °C. Chitosan from the squid pen is an excellent biomaterial candidate for bone tissue engineering applications.
26187191	9	38	contain	had	1286:1288	arg2	properties					1308:1317	higher mechanical properties	1290:1317	higher mechanical properties	1290:1317	The results of this study indicated that composites produced at -20 °C had higher mechanical properties and lower degradation rate compared with -80 °C. Chitosan from the squid pen is an excellent biomaterial candidate for bone tissue engineering applications.
26187191	9	38	contain	had	1286:1288	arg2	rate					1341:1344	lower degradation rate	1323:1344	lower degradation rate	1323:1344	The results of this study indicated that composites produced at -20 °C had higher mechanical properties and lower degradation rate compared with -80 °C. Chitosan from the squid pen is an excellent biomaterial candidate for bone tissue engineering applications.
26187191	0	39	from	chitosan	70:77	arg1	scaffold					22:29	Bio-mimetic composite scaffold	0:29	Bio-mimetic composite scaffold from mussel shells, squid pen and crab chitosan for bone tissue engineering.	0:106	Bio-mimetic composite scaffold from mussel shells, squid pen and crab chitosan for bone tissue engineering.
26187191	9	40	theme	lower	1323:1327	arg1	rate					1341:1344	lower degradation rate	1323:1344	lower degradation rate	1323:1344	The results of this study indicated that composites produced at -20 °C had higher mechanical properties and lower degradation rate compared with -80 °C. Chitosan from the squid pen is an excellent biomaterial candidate for bone tissue engineering applications.
26187191	6	41	theme	in	826:827	arg1	study					835:839	preliminary in vitro study	814:839	preliminary in vitro study	814:839	A simulated body fluid (SBF) solution was used for preliminary in vitro study for 1, 7, 14 and 28 days and the composites were characterized by XRD, FTIR, TGA, SEM, μ-CT and ICP-MS.
26187191	1	42	theme	β-TCP	183:187	arg1	composites					190:199	chitosan/hydroxyapatite (HA)/β-tircalcium phosphate (β-TCP) composites	130:199	chitosan/hydroxyapatite (HA)/β-tircalcium phosphate (β-TCP) composites	130:199	In the present study, chitosan/hydroxyapatite (HA)/β-tircalcium phosphate (β-TCP) composites were produced using squid pen derived chitosan (CHS) and commercial crab derived chitosan (CHC).
26187191	5	43	theme	°C	689:690	arg1	temperatures					655:666	different freezing temperatures	636:666	different freezing temperatures of -20, -80 and -196 °C	636:690	The effect of different freezing temperatures of -20, -80 and -196 °C on the physicochemical characteristics of composites was investigated.
26187191	1	44	theme	commercial	258:267	arg1	CHC					292:294	CHC	292:294	CHC	292:294	In the present study, chitosan/hydroxyapatite (HA)/β-tircalcium phosphate (β-TCP) composites were produced using squid pen derived chitosan (CHS) and commercial crab derived chitosan (CHC).
26187191	1	44	theme	commercial	258:267	arg1	chitosan					282:289	commercial crab derived chitosan	258:289	commercial crab derived chitosan (CHC)	258:295	In the present study, chitosan/hydroxyapatite (HA)/β-tircalcium phosphate (β-TCP) composites were produced using squid pen derived chitosan (CHS) and commercial crab derived chitosan (CHC).
26187191	9	45	theme	squid	1386:1390	arg1	pen					1392:1394	the squid pen	1382:1394	the squid pen is an excellent biomaterial candidate for bone tissue engineering applications	1382:1473	The results of this study indicated that composites produced at -20 °C had higher mechanical properties and lower degradation rate compared with -80 °C. Chitosan from the squid pen is an excellent biomaterial candidate for bone tissue engineering applications.
26187191	0	46	theme	mussel	36:41	arg1	shells					43:48	mussel shells	36:48	mussel shells	36:48	Bio-mimetic composite scaffold from mussel shells, squid pen and crab chitosan for bone tissue engineering.
26187191	1	47	theme	crab	269:272	arg1	CHC					292:294	CHC	292:294	CHC	292:294	In the present study, chitosan/hydroxyapatite (HA)/β-tircalcium phosphate (β-TCP) composites were produced using squid pen derived chitosan (CHS) and commercial crab derived chitosan (CHC).
26187191	1	47	theme	crab	269:272	arg1	chitosan					282:289	commercial crab derived chitosan	258:289	commercial crab derived chitosan (CHC)	258:295	In the present study, chitosan/hydroxyapatite (HA)/β-tircalcium phosphate (β-TCP) composites were produced using squid pen derived chitosan (CHS) and commercial crab derived chitosan (CHC).
26187191	5	48	theme	different	636:644	arg1	temperatures					655:666	different freezing temperatures	636:666	different freezing temperatures of -20, -80 and -196 °C	636:690	The effect of different freezing temperatures of -20, -80 and -196 °C on the physicochemical characteristics of composites was investigated.
26187191	6	49	theme	fluid	780:784	arg1	solution					792:799	A simulated body fluid (SBF) solution	763:799	A simulated body fluid (SBF) solution	763:799	A simulated body fluid (SBF) solution was used for preliminary in vitro study for 1, 7, 14 and 28 days and the composites were characterized by XRD, FTIR, TGA, SEM, μ-CT and ICP-MS.
26187191	7	50	theme	water	980:984	arg1	retention					986:994	water retention	980:994	water retention	980:994	Porosity, pore size, water uptake; water retention abilities and in vitro degradations of the prepared composites were evaluated.
26187191	0	51	theme	squid	51:55	arg1	pen					57:59	squid pen	51:59	squid pen	51:59	Bio-mimetic composite scaffold from mussel shells, squid pen and crab chitosan for bone tissue engineering.
26187191	1	52	theme	derived	274:280	arg1	CHC					292:294	CHC	292:294	CHC	292:294	In the present study, chitosan/hydroxyapatite (HA)/β-tircalcium phosphate (β-TCP) composites were produced using squid pen derived chitosan (CHS) and commercial crab derived chitosan (CHC).
26187191	1	52	theme	derived	274:280	arg1	chitosan					282:289	commercial crab derived chitosan	258:289	commercial crab derived chitosan (CHC)	258:295	In the present study, chitosan/hydroxyapatite (HA)/β-tircalcium phosphate (β-TCP) composites were produced using squid pen derived chitosan (CHS) and commercial crab derived chitosan (CHC).
26187191	7	53	dep	in	1010:1011	arg1	vitro					1013:1017	vitro	1013:1017	vitro	1013:1017	Porosity, pore size, water uptake; water retention abilities and in vitro degradations of the prepared composites were evaluated.
26187191	0	54	from	pen	57:59	arg1	scaffold					22:29	Bio-mimetic composite scaffold	0:29	Bio-mimetic composite scaffold from mussel shells, squid pen and crab chitosan for bone tissue engineering.	0:106	Bio-mimetic composite scaffold from mussel shells, squid pen and crab chitosan for bone tissue engineering.
26187191	8	55	theme	mechanical	1192:1201	arg1	properties					1203:1212	better mechanical properties	1185:1212	better mechanical properties	1185:1212	The CHS composites were found to have higher porosity (62%) compared to the CHC composites (porosity 42%) and better mechanical properties.
26187191	3	56	theme	microwave	415:423	arg1	irradiation					425:435	a microwave irradiation	413:435	a microwave irradiation method	413:442	HA and β-TCP were extracted from mussel shells using a microwave irradiation method.
26187191	5	57	theme	physicochemical	699:713	arg1	characteristics					715:729	the physicochemical characteristics	695:729	the physicochemical characteristics of composites	695:743	The effect of different freezing temperatures of -20, -80 and -196 °C on the physicochemical characteristics of composites was investigated.
26187191	4	58	theme	%	503:503	arg1	β-TCP					515:519	β-TCP	515:519	β-TCP	515:519	Two different composites were prepared by incorporating 50% (w/w) HA/(β-TCP) in CHS or CHC followed by lyophilization and cross-linking of composites by tripolyphosphate (TPP).
26187191	4	58	theme	%	503:503	arg1	HA/					511:513	50% (w/w) HA/	501:513	50% (w/w) HA/(β-TCP)	501:520	Two different composites were prepared by incorporating 50% (w/w) HA/(β-TCP) in CHS or CHC followed by lyophilization and cross-linking of composites by tripolyphosphate (TPP).
26187191	6	59	theme	simulated	765:773	arg1	fluid					780:784	A simulated body fluid	763:784	A simulated body fluid (SBF) solution	763:799	A simulated body fluid (SBF) solution was used for preliminary in vitro study for 1, 7, 14 and 28 days and the composites were characterized by XRD, FTIR, TGA, SEM, μ-CT and ICP-MS.
26187191	6	59	theme	simulated	765:773	arg1	SBF					787:789	SBF	787:789	SBF	787:789	A simulated body fluid (SBF) solution was used for preliminary in vitro study for 1, 7, 14 and 28 days and the composites were characterized by XRD, FTIR, TGA, SEM, μ-CT and ICP-MS.
26187191	8	60	theme	better	1185:1190	arg1	properties					1203:1212	better mechanical properties	1185:1212	better mechanical properties	1185:1212	The CHS composites were found to have higher porosity (62%) compared to the CHC composites (porosity 42%) and better mechanical properties.
26187191	9	61	theme	tissue	1443:1448	arg1	applications					1462:1473	bone tissue engineering applications	1438:1473	bone tissue engineering applications	1438:1473	The results of this study indicated that composites produced at -20 °C had higher mechanical properties and lower degradation rate compared with -80 °C. Chitosan from the squid pen is an excellent biomaterial candidate for bone tissue engineering applications.
26187191	8	62	contain	have	1108:1111	arg2	%					1132:1132	62%	1130:1132	62%	1130:1132	The CHS composites were found to have higher porosity (62%) compared to the CHC composites (porosity 42%) and better mechanical properties.
26187191	8	62	contain	have	1108:1111	arg1	composites					1083:1092	The CHS composites	1075:1092	The CHS composites	1075:1092	The CHS composites were found to have higher porosity (62%) compared to the CHC composites (porosity 42%) and better mechanical properties.
26187191	8	62	contain	have	1108:1111	arg2	porosity					1120:1127	higher porosity	1113:1127	higher porosity (62%)	1113:1133	The CHS composites were found to have higher porosity (62%) compared to the CHC composites (porosity 42%) and better mechanical properties.
26187191	7	63	theme	water	966:970	arg1	uptake					972:977	water uptake	966:977	water uptake	966:977	Porosity, pore size, water uptake; water retention abilities and in vitro degradations of the prepared composites were evaluated.
26187191	7	63	theme	water	966:970	arg1	Porosity					945:952	Porosity	945:952	Porosity	945:952	Porosity, pore size, water uptake; water retention abilities and in vitro degradations of the prepared composites were evaluated.
26187191	1	64	link	derived	274:280	arg1	CHC					292:294	CHC	292:294	CHC	292:294	In the present study, chitosan/hydroxyapatite (HA)/β-tircalcium phosphate (β-TCP) composites were produced using squid pen derived chitosan (CHS) and commercial crab derived chitosan (CHC).
26187191	1	64	link	derived	274:280	arg1	chitosan					282:289	commercial crab derived chitosan	258:289	commercial crab derived chitosan (CHC)	258:295	In the present study, chitosan/hydroxyapatite (HA)/β-tircalcium phosphate (β-TCP) composites were produced using squid pen derived chitosan (CHS) and commercial crab derived chitosan (CHC).
26187191	3	65	theme	irradiation	425:435	arg1	method					437:442	a microwave irradiation method	413:442	a microwave irradiation method	413:442	HA and β-TCP were extracted from mussel shells using a microwave irradiation method.
26187191	9	66	theme	excellent	1402:1410	arg1	candidate					1424:1432	an excellent biomaterial candidate	1399:1432	an excellent biomaterial candidate for bone tissue engineering applications	1399:1473	The results of this study indicated that composites produced at -20 °C had higher mechanical properties and lower degradation rate compared with -80 °C. Chitosan from the squid pen is an excellent biomaterial candidate for bone tissue engineering applications.
26187191	1	67	theme	present	115:121	arg1	study					123:127	the present study	111:127	the present study	111:127	In the present study, chitosan/hydroxyapatite (HA)/β-tircalcium phosphate (β-TCP) composites were produced using squid pen derived chitosan (CHS) and commercial crab derived chitosan (CHC).
26187191	7	68	theme	composites	1048:1057	arg1	degradations					1019:1030	in vitro degradations	1010:1030	in vitro degradations	1010:1030	Porosity, pore size, water uptake; water retention abilities and in vitro degradations of the prepared composites were evaluated.
26187191	7	68	theme	composites	1048:1057	arg1	abilities					996:1004	Porosity, pore size, water uptake; water retention abilities	945:1004	Porosity, pore size, water uptake; water retention abilities	945:1004	Porosity, pore size, water uptake; water retention abilities and in vitro degradations of the prepared composites were evaluated.
26187191	9	69	theme	biomaterial	1412:1422	arg1	candidate					1424:1432	an excellent biomaterial candidate	1399:1432	an excellent biomaterial candidate for bone tissue engineering applications	1399:1473	The results of this study indicated that composites produced at -20 °C had higher mechanical properties and lower degradation rate compared with -80 °C. Chitosan from the squid pen is an excellent biomaterial candidate for bone tissue engineering applications.
26187191	9	70	theme	mechanical	1297:1306	arg1	properties					1308:1317	higher mechanical properties	1290:1317	higher mechanical properties	1290:1317	The results of this study indicated that composites produced at -20 °C had higher mechanical properties and lower degradation rate compared with -80 °C. Chitosan from the squid pen is an excellent biomaterial candidate for bone tissue engineering applications.
26187191	2	71	theme	squid	320:324	arg1	pens					326:329	squid pens	320:329	squid pens	320:329	CHS was prepared from squid pens by alkaline N-deacetylation.
26187191	3	72	theme	mussel	393:398	arg1	shells					400:405	mussel shells	393:405	mussel shells	393:405	HA and β-TCP were extracted from mussel shells using a microwave irradiation method.
26187191	9	73	theme	study	1235:1239	arg1	results					1219:1225	The results	1215:1225	The results of this study	1215:1239	The results of this study indicated that composites produced at -20 °C had higher mechanical properties and lower degradation rate compared with -80 °C. Chitosan from the squid pen is an excellent biomaterial candidate for bone tissue engineering applications.
26187191	7	74	dep	Porosity	945:952	arg1	retention					986:994	water retention	980:994	water retention	980:994	Porosity, pore size, water uptake; water retention abilities and in vitro degradations of the prepared composites were evaluated.
26187191	1	75	theme	squid	221:225	arg1	CHS					249:251	CHS	249:251	CHS	249:251	In the present study, chitosan/hydroxyapatite (HA)/β-tircalcium phosphate (β-TCP) composites were produced using squid pen derived chitosan (CHS) and commercial crab derived chitosan (CHC).
26187191	1	75	theme	squid	221:225	arg1	chitosan					239:246	squid pen derived chitosan	221:246	squid pen derived chitosan (CHS)	221:252	In the present study, chitosan/hydroxyapatite (HA)/β-tircalcium phosphate (β-TCP) composites were produced using squid pen derived chitosan (CHS) and commercial crab derived chitosan (CHC).
27825861	3	0	theme	aqueous	700:706	arg1	films					748:752	aqueous ternary polymer-drug-polymer nanomatrix films	700:752	aqueous ternary polymer-drug-polymer nanomatrix films	700:752	The focus of this study was to investigate the impacts of the in situ formation of polymer-drug nanoconjugate, at the interfacial nano-domains of two oppositely charged polymers, on the mechanism of film formation and to prepare aqueous ternary polymer-drug-polymer nanomatrix films as a novel green strategy for the delivery of ibuprofen, a model poorly soluble drug.
27825861	3	0	theme	aqueous	700:706	arg1	strategy					771:778	a novel green strategy	757:778	a novel green strategy for the delivery of ibuprofen, a model poorly soluble drug	757:837	The focus of this study was to investigate the impacts of the in situ formation of polymer-drug nanoconjugate, at the interfacial nano-domains of two oppositely charged polymers, on the mechanism of film formation and to prepare aqueous ternary polymer-drug-polymer nanomatrix films as a novel green strategy for the delivery of ibuprofen, a model poorly soluble drug.
27825861	1	1	theme	masking	241:247	arg1	strategies					249:258	taste masking strategies	235:258	taste masking strategies	235:258	Aqueous polymeric films have potentially great values in drug development, particularly in controlled drug release and taste masking strategies.
27825861	9	2	theme	release	1642:1648	arg1	mechanism					1624:1632	the mechanism	1620:1632	the mechanism of drug release	1620:1648	Generally, the mechanism of drug release was by Fickian diffusion, however anomalous transport or polymer relaxation was also observed at higher concentration of Ddex.
27825861	6	3	theme	gellan	1177:1182	arg1	film					1184:1187	gellan film	1177:1187	gellan film	1177:1187	Ibuprofen formed spherical core-shell microstructures (4.55-9.73μm) in gellan film.
27825861	10	4	theme	model	1921:1925	arg1	ibuprofen					1908:1916	ibuprofen	1908:1916	ibuprofen	1908:1916	This study demonstrated the potential application of aqueous drug-loaded nanomatrix films as controlled drug delivery strategy for ibuprofen, a model poorly soluble drug.
27825861	10	4	theme	model	1921:1925	arg1	drug					1942:1945	a model poorly soluble drug	1919:1945	a model poorly soluble drug	1919:1945	This study demonstrated the potential application of aqueous drug-loaded nanomatrix films as controlled drug delivery strategy for ibuprofen, a model poorly soluble drug.
27825861	8	5	dep	exhibited	1353:1361	arg1	compared					1527:1534	compared	1527:1534	compared to 47.37% in layer-by-layer (LbL) films, dictated by Ddex concentration	1527:1606	The composite films exhibited reduced tensile strength and lower elastic modulus with optimal conjugation efficiency of 98.14±1.19%, which correlates with higher dissolution efficiency (99.76%) compared to 47.37% in layer-by-layer (LbL) films, dictated by Ddex concentration.
27825861	3	6	theme	polymer-drug-polymer	716:735	arg1	films					748:752	aqueous ternary polymer-drug-polymer nanomatrix films	700:752	aqueous ternary polymer-drug-polymer nanomatrix films	700:752	The focus of this study was to investigate the impacts of the in situ formation of polymer-drug nanoconjugate, at the interfacial nano-domains of two oppositely charged polymers, on the mechanism of film formation and to prepare aqueous ternary polymer-drug-polymer nanomatrix films as a novel green strategy for the delivery of ibuprofen, a model poorly soluble drug.
27825861	3	6	theme	polymer-drug-polymer	716:735	arg1	strategy					771:778	a novel green strategy	757:778	a novel green strategy for the delivery of ibuprofen, a model poorly soluble drug	757:837	The focus of this study was to investigate the impacts of the in situ formation of polymer-drug nanoconjugate, at the interfacial nano-domains of two oppositely charged polymers, on the mechanism of film formation and to prepare aqueous ternary polymer-drug-polymer nanomatrix films as a novel green strategy for the delivery of ibuprofen, a model poorly soluble drug.
27825861	2	7	theme	progressive	273:283	arg1	coalescence					302:312	the progressive polymer-particle coalescence	269:312	the progressive polymer-particle coalescence that occurs randomly during film formation, curing and storage	269:375	However the progressive polymer-particle coalescence that occurs randomly during film formation, curing and storage may render the film less permeable leading to erratic and unpredictable drug release profile.
27825861	1	8	theme	drug	173:176	arg1	development					178:188	drug development	173:188	drug development	173:188	Aqueous polymeric films have potentially great values in drug development, particularly in controlled drug release and taste masking strategies.
27825861	3	9	theme	green	765:769	arg1	films					748:752	aqueous ternary polymer-drug-polymer nanomatrix films	700:752	aqueous ternary polymer-drug-polymer nanomatrix films	700:752	The focus of this study was to investigate the impacts of the in situ formation of polymer-drug nanoconjugate, at the interfacial nano-domains of two oppositely charged polymers, on the mechanism of film formation and to prepare aqueous ternary polymer-drug-polymer nanomatrix films as a novel green strategy for the delivery of ibuprofen, a model poorly soluble drug.
27825861	3	9	theme	green	765:769	arg1	strategy					771:778	a novel green strategy	757:778	a novel green strategy for the delivery of ibuprofen, a model poorly soluble drug	757:837	The focus of this study was to investigate the impacts of the in situ formation of polymer-drug nanoconjugate, at the interfacial nano-domains of two oppositely charged polymers, on the mechanism of film formation and to prepare aqueous ternary polymer-drug-polymer nanomatrix films as a novel green strategy for the delivery of ibuprofen, a model poorly soluble drug.
27825861	0	10	theme	soluble	101:107	arg1	drugs					109:113	poorly soluble drugs	94:113	poorly soluble drugs	94:113	Development of aqueous ternary nanomatrix films: A novel 'green' strategy for the delivery of poorly soluble drugs.
27825861	8	11	theme	%	1463:1463	arg1	efficiency					1439:1448	optimal conjugation efficiency	1419:1448	optimal conjugation efficiency of 98.14±1.19%, which correlates with higher dissolution efficiency (99.76%)	1419:1525	The composite films exhibited reduced tensile strength and lower elastic modulus with optimal conjugation efficiency of 98.14±1.19%, which correlates with higher dissolution efficiency (99.76%) compared to 47.37% in layer-by-layer (LbL) films, dictated by Ddex concentration.
27825861	4	12	theme	aqueous	892:898	arg1	technique					908:916	aqueous casting technique	892:916	aqueous casting technique using the concept of combined polymer-drug self-assembly and polyelectrolyte complexation	892:1006	Composite and Layer-by-Layer films were prepared by aqueous casting technique using the concept of combined polymer-drug self-assembly and polyelectrolyte complexation.
27825861	3	13	theme	nanoconjugate	567:579	arg1	formation					541:549	the in situ formation	529:549	the in situ formation of polymer-drug nanoconjugate	529:579	The focus of this study was to investigate the impacts of the in situ formation of polymer-drug nanoconjugate, at the interfacial nano-domains of two oppositely charged polymers, on the mechanism of film formation and to prepare aqueous ternary polymer-drug-polymer nanomatrix films as a novel green strategy for the delivery of ibuprofen, a model poorly soluble drug.
27825861	9	14	theme	polymer	1707:1713	arg1	relaxation					1715:1724	polymer relaxation	1707:1724	polymer relaxation	1707:1724	Generally, the mechanism of drug release was by Fickian diffusion, however anomalous transport or polymer relaxation was also observed at higher concentration of Ddex.
27825861	0	15	theme	novel	51:55	arg1	strategy					65:72	A novel 'green' strategy	49:72	Development of aqueous ternary nanomatrix films: A novel 'green' strategy for the delivery of poorly soluble drugs.	0:114	Development of aqueous ternary nanomatrix films: A novel 'green' strategy for the delivery of poorly soluble drugs.
27825861	3	16	theme	interfacial	589:599	arg1	polymers					640:647	two oppositely charged polymers	617:647	two oppositely charged polymers	617:647	The focus of this study was to investigate the impacts of the in situ formation of polymer-drug nanoconjugate, at the interfacial nano-domains of two oppositely charged polymers, on the mechanism of film formation and to prepare aqueous ternary polymer-drug-polymer nanomatrix films as a novel green strategy for the delivery of ibuprofen, a model poorly soluble drug.
27825861	3	16	theme	interfacial	589:599	arg1	nano-domains					601:612	the interfacial nano-domains	585:612	the interfacial nano-domains of two oppositely charged polymers	585:647	The focus of this study was to investigate the impacts of the in situ formation of polymer-drug nanoconjugate, at the interfacial nano-domains of two oppositely charged polymers, on the mechanism of film formation and to prepare aqueous ternary polymer-drug-polymer nanomatrix films as a novel green strategy for the delivery of ibuprofen, a model poorly soluble drug.
27825861	1	17	theme	Aqueous	116:122	arg1	films					134:138	Aqueous polymeric films	116:138	Aqueous polymeric films	116:138	Aqueous polymeric films have potentially great values in drug development, particularly in controlled drug release and taste masking strategies.
27825861	4	18	theme	complexation	995:1006	arg1	concept					928:934	the concept	924:934	the concept of combined polymer-drug self-assembly and polyelectrolyte complexation	924:1006	Composite and Layer-by-Layer films were prepared by aqueous casting technique using the concept of combined polymer-drug self-assembly and polyelectrolyte complexation.
27825861	8	19	theme	dissolution	1495:1505	arg1	efficiency					1507:1516	higher dissolution efficiency	1488:1516	higher dissolution efficiency (99.76%)	1488:1525	The composite films exhibited reduced tensile strength and lower elastic modulus with optimal conjugation efficiency of 98.14±1.19%, which correlates with higher dissolution efficiency (99.76%) compared to 47.37% in layer-by-layer (LbL) films, dictated by Ddex concentration.
27825861	8	19	theme	dissolution	1495:1505	arg1	%					1524:1524	99.76%	1519:1524	99.76%	1519:1524	The composite films exhibited reduced tensile strength and lower elastic modulus with optimal conjugation efficiency of 98.14±1.19%, which correlates with higher dissolution efficiency (99.76%) compared to 47.37% in layer-by-layer (LbL) films, dictated by Ddex concentration.
27825861	10	20	theme	soluble	1934:1940	arg1	ibuprofen					1908:1916	ibuprofen	1908:1916	ibuprofen	1908:1916	This study demonstrated the potential application of aqueous drug-loaded nanomatrix films as controlled drug delivery strategy for ibuprofen, a model poorly soluble drug.
27825861	10	20	theme	soluble	1934:1940	arg1	drug					1942:1945	a model poorly soluble drug	1919:1945	a model poorly soluble drug	1919:1945	This study demonstrated the potential application of aqueous drug-loaded nanomatrix films as controlled drug delivery strategy for ibuprofen, a model poorly soluble drug.
27825861	7	21	theme	cationic	1217:1224	arg1	Ddex					1235:1238	Ddex	1235:1238	Ddex	1235:1238	However in the presence of cationic dextran (Ddex), nanoconjugates (61.49±5.97-447.52±37.51nm) were formed within the core of the film matrix.
27825861	7	21	theme	cationic	1217:1224	arg1	dextran					1226:1232	cationic dextran	1217:1232	cationic dextran (Ddex)	1217:1239	However in the presence of cationic dextran (Ddex), nanoconjugates (61.49±5.97-447.52±37.51nm) were formed within the core of the film matrix.
27825861	4	22	theme	Layer-by-Layer	854:867	arg1	films					869:873	Composite and Layer-by-Layer films	840:873	Composite and Layer-by-Layer films	840:873	Composite and Layer-by-Layer films were prepared by aqueous casting technique using the concept of combined polymer-drug self-assembly and polyelectrolyte complexation.
27825861	10	23	theme	films	1861:1865	arg1	application					1815:1825	the potential application	1801:1825	the potential application of aqueous drug-loaded nanomatrix films	1801:1865	This study demonstrated the potential application of aqueous drug-loaded nanomatrix films as controlled drug delivery strategy for ibuprofen, a model poorly soluble drug.
27825861	9	24	theme	higher	1747:1752	arg1	concentration					1754:1766	higher concentration	1747:1766	higher concentration of Ddex	1747:1774	Generally, the mechanism of drug release was by Fickian diffusion, however anomalous transport or polymer relaxation was also observed at higher concentration of Ddex.
27825861	3	25	theme	ibuprofen	800:808	arg1	delivery					788:795	the delivery	784:795	the delivery of ibuprofen, a model poorly soluble drug	784:837	The focus of this study was to investigate the impacts of the in situ formation of polymer-drug nanoconjugate, at the interfacial nano-domains of two oppositely charged polymers, on the mechanism of film formation and to prepare aqueous ternary polymer-drug-polymer nanomatrix films as a novel green strategy for the delivery of ibuprofen, a model poorly soluble drug.
27825861	4	26	theme	polymer-drug	948:959	arg1	self-assembly					961:973	combined polymer-drug self-assembly	939:973	combined polymer-drug self-assembly	939:973	Composite and Layer-by-Layer films were prepared by aqueous casting technique using the concept of combined polymer-drug self-assembly and polyelectrolyte complexation.
27825861	9	27	located	observed	1735:1742	arg2	relaxation					1715:1724	polymer relaxation	1707:1724	polymer relaxation	1707:1724	Generally, the mechanism of drug release was by Fickian diffusion, however anomalous transport or polymer relaxation was also observed at higher concentration of Ddex.
27825861	9	27	located	observed	1735:1742	arg1	concentration					1754:1766	higher concentration	1747:1766	higher concentration of Ddex	1747:1774	Generally, the mechanism of drug release was by Fickian diffusion, however anomalous transport or polymer relaxation was also observed at higher concentration of Ddex.
27825861	9	27	located	observed	1735:1742	arg2	transport					1694:1702	anomalous transport	1684:1702	anomalous transport	1684:1702	Generally, the mechanism of drug release was by Fickian diffusion, however anomalous transport or polymer relaxation was also observed at higher concentration of Ddex.
27825861	7	28	theme	matrix	1325:1330	arg1	core					1308:1311	the core	1304:1311	the core of the film matrix	1304:1330	However in the presence of cationic dextran (Ddex), nanoconjugates (61.49±5.97-447.52±37.51nm) were formed within the core of the film matrix.
27825861	2	29	theme	unpredictable	435:447	arg1	profile					462:468	erratic and unpredictable drug release profile	423:468	erratic and unpredictable drug release profile	423:468	However the progressive polymer-particle coalescence that occurs randomly during film formation, curing and storage may render the film less permeable leading to erratic and unpredictable drug release profile.
27825861	8	30	theme	reduced	1363:1369	arg1	strength					1379:1386	reduced tensile strength	1363:1386	reduced tensile strength	1363:1386	The composite films exhibited reduced tensile strength and lower elastic modulus with optimal conjugation efficiency of 98.14±1.19%, which correlates with higher dissolution efficiency (99.76%) compared to 47.37% in layer-by-layer (LbL) films, dictated by Ddex concentration.
27825861	10	31	theme	nanomatrix	1850:1859	arg1	films					1861:1865	aqueous drug-loaded nanomatrix films	1830:1865	aqueous drug-loaded nanomatrix films	1830:1865	This study demonstrated the potential application of aqueous drug-loaded nanomatrix films as controlled drug delivery strategy for ibuprofen, a model poorly soluble drug.
27825861	2	32	theme	erratic	423:429	arg1	profile					462:468	erratic and unpredictable drug release profile	423:468	erratic and unpredictable drug release profile	423:468	However the progressive polymer-particle coalescence that occurs randomly during film formation, curing and storage may render the film less permeable leading to erratic and unpredictable drug release profile.
27825861	0	33	theme	aqueous	15:21	arg1	films					42:46	aqueous ternary nanomatrix films	15:46	aqueous ternary nanomatrix films	15:46	Development of aqueous ternary nanomatrix films: A novel 'green' strategy for the delivery of poorly soluble drugs.
27825861	0	34	dep	novel	51:55	arg1	green					58:62	green	58:62	green	58:62	Development of aqueous ternary nanomatrix films: A novel 'green' strategy for the delivery of poorly soluble drugs.
27825861	7	35	dep	dextran	1226:1232	arg1	the					1201:1203	the	1201:1203	the	1201:1203	However in the presence of cationic dextran (Ddex), nanoconjugates (61.49±5.97-447.52±37.51nm) were formed within the core of the film matrix.
27825861	7	35	dep	dextran	1226:1232	arg1	presence					1205:1212	presence	1205:1212	presence	1205:1212	However in the presence of cationic dextran (Ddex), nanoconjugates (61.49±5.97-447.52±37.51nm) were formed within the core of the film matrix.
27825861	3	36	theme	polymers	640:647	arg1	polymers					640:647	two oppositely charged polymers	617:647	two oppositely charged polymers	617:647	The focus of this study was to investigate the impacts of the in situ formation of polymer-drug nanoconjugate, at the interfacial nano-domains of two oppositely charged polymers, on the mechanism of film formation and to prepare aqueous ternary polymer-drug-polymer nanomatrix films as a novel green strategy for the delivery of ibuprofen, a model poorly soluble drug.
27825861	3	36	theme	polymers	640:647	arg1	nano-domains					601:612	the interfacial nano-domains	585:612	the interfacial nano-domains of two oppositely charged polymers	585:647	The focus of this study was to investigate the impacts of the in situ formation of polymer-drug nanoconjugate, at the interfacial nano-domains of two oppositely charged polymers, on the mechanism of film formation and to prepare aqueous ternary polymer-drug-polymer nanomatrix films as a novel green strategy for the delivery of ibuprofen, a model poorly soluble drug.
27825861	8	37	theme	lower	1392:1396	arg1	modulus					1406:1412	lower elastic modulus	1392:1412	lower elastic modulus	1392:1412	The composite films exhibited reduced tensile strength and lower elastic modulus with optimal conjugation efficiency of 98.14±1.19%, which correlates with higher dissolution efficiency (99.76%) compared to 47.37% in layer-by-layer (LbL) films, dictated by Ddex concentration.
27825861	0	38	theme	nanomatrix	31:40	arg1	films					42:46	aqueous ternary nanomatrix films	15:46	aqueous ternary nanomatrix films	15:46	Development of aqueous ternary nanomatrix films: A novel 'green' strategy for the delivery of poorly soluble drugs.
27825861	5	39	theme	nanomatrix	1035:1044	arg1	films					1046:1050	The plain and drug-loaded nanomatrix films	1009:1050	films	1046:1050	The plain and drug-loaded nanomatrix films were characterized using SEM, AFM, FTIR, DSC and TGA.
27825861	2	40	theme	release	454:460	arg1	profile					462:468	erratic and unpredictable drug release profile	423:468	erratic and unpredictable drug release profile	423:468	However the progressive polymer-particle coalescence that occurs randomly during film formation, curing and storage may render the film less permeable leading to erratic and unpredictable drug release profile.
27825861	1	41	contain	have	140:143	arg2	values					163:168	potentially great values	145:168	potentially great values	145:168	Aqueous polymeric films have potentially great values in drug development, particularly in controlled drug release and taste masking strategies.
27825861	1	41	contain	have	140:143	arg1	films					134:138	Aqueous polymeric films	116:138	Aqueous polymeric films	116:138	Aqueous polymeric films have potentially great values in drug development, particularly in controlled drug release and taste masking strategies.
27825861	2	42	theme	film	342:345	arg1	formation					347:355	film formation	342:355	film formation	342:355	However the progressive polymer-particle coalescence that occurs randomly during film formation, curing and storage may render the film less permeable leading to erratic and unpredictable drug release profile.
27825861	6	43	theme	spherical	1123:1131	arg1	4.55-9.73μm					1161:1171	4.55-9.73μm	1161:1171	4.55-9.73μm	1161:1171	Ibuprofen formed spherical core-shell microstructures (4.55-9.73μm) in gellan film.
27825861	6	43	theme	spherical	1123:1131	arg1	microstructures					1144:1158	spherical core-shell microstructures	1123:1158	spherical core-shell microstructures (4.55-9.73μm)	1123:1172	Ibuprofen formed spherical core-shell microstructures (4.55-9.73μm) in gellan film.
27825861	10	44	theme	drug	1881:1884	arg1	strategy					1895:1902	controlled drug delivery strategy	1870:1902	controlled drug delivery strategy for ibuprofen, a model poorly soluble drug	1870:1945	This study demonstrated the potential application of aqueous drug-loaded nanomatrix films as controlled drug delivery strategy for ibuprofen, a model poorly soluble drug.
27825861	8	45	theme	optimal	1419:1425	arg1	efficiency					1439:1448	optimal conjugation efficiency	1419:1448	optimal conjugation efficiency of 98.14±1.19%, which correlates with higher dissolution efficiency (99.76%)	1419:1525	The composite films exhibited reduced tensile strength and lower elastic modulus with optimal conjugation efficiency of 98.14±1.19%, which correlates with higher dissolution efficiency (99.76%) compared to 47.37% in layer-by-layer (LbL) films, dictated by Ddex concentration.
27825861	1	46	theme	controlled	207:216	arg1	release					223:229	controlled drug release	207:229	controlled drug release	207:229	Aqueous polymeric films have potentially great values in drug development, particularly in controlled drug release and taste masking strategies.
27825861	0	47	dep	Development	0:10	arg1	strategy					65:72	A novel 'green' strategy	49:72	Development of aqueous ternary nanomatrix films: A novel 'green' strategy for the delivery of poorly soluble drugs.	0:114	Development of aqueous ternary nanomatrix films: A novel 'green' strategy for the delivery of poorly soluble drugs.
27825861	3	48	theme	study	489:493	arg1	focus					475:479	The focus	471:479	The focus of this study	471:493	The focus of this study was to investigate the impacts of the in situ formation of polymer-drug nanoconjugate, at the interfacial nano-domains of two oppositely charged polymers, on the mechanism of film formation and to prepare aqueous ternary polymer-drug-polymer nanomatrix films as a novel green strategy for the delivery of ibuprofen, a model poorly soluble drug.
27825861	4	49	theme	Composite	840:848	arg1	films					869:873	Composite and Layer-by-Layer films	840:873	Composite and Layer-by-Layer films	840:873	Composite and Layer-by-Layer films were prepared by aqueous casting technique using the concept of combined polymer-drug self-assembly and polyelectrolyte complexation.
27825861	10	50	theme	aqueous	1830:1836	arg1	films					1861:1865	aqueous drug-loaded nanomatrix films	1830:1865	aqueous drug-loaded nanomatrix films	1830:1865	This study demonstrated the potential application of aqueous drug-loaded nanomatrix films as controlled drug delivery strategy for ibuprofen, a model poorly soluble drug.
27825861	3	51	theme	film	670:673	arg1	formation					675:683	film formation	670:683	film formation	670:683	The focus of this study was to investigate the impacts of the in situ formation of polymer-drug nanoconjugate, at the interfacial nano-domains of two oppositely charged polymers, on the mechanism of film formation and to prepare aqueous ternary polymer-drug-polymer nanomatrix films as a novel green strategy for the delivery of ibuprofen, a model poorly soluble drug.
27825861	8	52	from	%	1544:1544	arg1	films					1570:1574	layer-by-layer (LbL) films	1549:1574	layer-by-layer (LbL) films	1549:1574	The composite films exhibited reduced tensile strength and lower elastic modulus with optimal conjugation efficiency of 98.14±1.19%, which correlates with higher dissolution efficiency (99.76%) compared to 47.37% in layer-by-layer (LbL) films, dictated by Ddex concentration.
27825861	1	53	theme	taste	235:239	arg1	strategies					249:258	taste masking strategies	235:258	taste masking strategies	235:258	Aqueous polymeric films have potentially great values in drug development, particularly in controlled drug release and taste masking strategies.
27825861	9	54	theme	drug	1637:1640	arg1	release					1642:1648	drug release	1637:1648	drug release	1637:1648	Generally, the mechanism of drug release was by Fickian diffusion, however anomalous transport or polymer relaxation was also observed at higher concentration of Ddex.
27825861	3	55	theme	ternary	708:714	arg1	films					748:752	aqueous ternary polymer-drug-polymer nanomatrix films	700:752	aqueous ternary polymer-drug-polymer nanomatrix films	700:752	The focus of this study was to investigate the impacts of the in situ formation of polymer-drug nanoconjugate, at the interfacial nano-domains of two oppositely charged polymers, on the mechanism of film formation and to prepare aqueous ternary polymer-drug-polymer nanomatrix films as a novel green strategy for the delivery of ibuprofen, a model poorly soluble drug.
27825861	3	55	theme	ternary	708:714	arg1	strategy					771:778	a novel green strategy	757:778	a novel green strategy for the delivery of ibuprofen, a model poorly soluble drug	757:837	The focus of this study was to investigate the impacts of the in situ formation of polymer-drug nanoconjugate, at the interfacial nano-domains of two oppositely charged polymers, on the mechanism of film formation and to prepare aqueous ternary polymer-drug-polymer nanomatrix films as a novel green strategy for the delivery of ibuprofen, a model poorly soluble drug.
27825861	3	56	theme	formation	541:549	arg1	impacts					518:524	the impacts	514:524	the impacts of the in situ formation of polymer-drug nanoconjugate	514:579	The focus of this study was to investigate the impacts of the in situ formation of polymer-drug nanoconjugate, at the interfacial nano-domains of two oppositely charged polymers, on the mechanism of film formation and to prepare aqueous ternary polymer-drug-polymer nanomatrix films as a novel green strategy for the delivery of ibuprofen, a model poorly soluble drug.
27825861	1	57	theme	great	157:161	arg1	values					163:168	potentially great values	145:168	potentially great values	145:168	Aqueous polymeric films have potentially great values in drug development, particularly in controlled drug release and taste masking strategies.
27825861	9	58	theme	Fickian	1657:1663	arg1	diffusion					1665:1673	Fickian diffusion	1657:1673	Fickian diffusion	1657:1673	Generally, the mechanism of drug release was by Fickian diffusion, however anomalous transport or polymer relaxation was also observed at higher concentration of Ddex.
27825861	2	59	theme	polymer-particle	285:300	arg1	coalescence					302:312	the progressive polymer-particle coalescence	269:312	the progressive polymer-particle coalescence that occurs randomly during film formation, curing and storage	269:375	However the progressive polymer-particle coalescence that occurs randomly during film formation, curing and storage may render the film less permeable leading to erratic and unpredictable drug release profile.
27825861	4	60	theme	casting	900:906	arg1	technique					908:916	aqueous casting technique	892:916	aqueous casting technique using the concept of combined polymer-drug self-assembly and polyelectrolyte complexation	892:1006	Composite and Layer-by-Layer films were prepared by aqueous casting technique using the concept of combined polymer-drug self-assembly and polyelectrolyte complexation.
27825861	3	61	theme	nanomatrix	737:746	arg1	films					748:752	aqueous ternary polymer-drug-polymer nanomatrix films	700:752	aqueous ternary polymer-drug-polymer nanomatrix films	700:752	The focus of this study was to investigate the impacts of the in situ formation of polymer-drug nanoconjugate, at the interfacial nano-domains of two oppositely charged polymers, on the mechanism of film formation and to prepare aqueous ternary polymer-drug-polymer nanomatrix films as a novel green strategy for the delivery of ibuprofen, a model poorly soluble drug.
27825861	3	61	theme	nanomatrix	737:746	arg1	strategy					771:778	a novel green strategy	757:778	a novel green strategy for the delivery of ibuprofen, a model poorly soluble drug	757:837	The focus of this study was to investigate the impacts of the in situ formation of polymer-drug nanoconjugate, at the interfacial nano-domains of two oppositely charged polymers, on the mechanism of film formation and to prepare aqueous ternary polymer-drug-polymer nanomatrix films as a novel green strategy for the delivery of ibuprofen, a model poorly soluble drug.
27825861	0	62	theme	drugs	109:113	arg1	delivery					82:89	the delivery	78:89	the delivery of poorly soluble drugs	78:113	Development of aqueous ternary nanomatrix films: A novel 'green' strategy for the delivery of poorly soluble drugs.
27825861	9	63	theme	anomalous	1684:1692	arg1	transport					1694:1702	anomalous transport	1684:1702	anomalous transport	1684:1702	Generally, the mechanism of drug release was by Fickian diffusion, however anomalous transport or polymer relaxation was also observed at higher concentration of Ddex.
27825861	3	64	theme	polymer-drug	554:565	arg1	nanoconjugate					567:579	polymer-drug nanoconjugate	554:579	polymer-drug nanoconjugate	554:579	The focus of this study was to investigate the impacts of the in situ formation of polymer-drug nanoconjugate, at the interfacial nano-domains of two oppositely charged polymers, on the mechanism of film formation and to prepare aqueous ternary polymer-drug-polymer nanomatrix films as a novel green strategy for the delivery of ibuprofen, a model poorly soluble drug.
27825861	3	65	theme	novel	759:763	arg1	films					748:752	aqueous ternary polymer-drug-polymer nanomatrix films	700:752	aqueous ternary polymer-drug-polymer nanomatrix films	700:752	The focus of this study was to investigate the impacts of the in situ formation of polymer-drug nanoconjugate, at the interfacial nano-domains of two oppositely charged polymers, on the mechanism of film formation and to prepare aqueous ternary polymer-drug-polymer nanomatrix films as a novel green strategy for the delivery of ibuprofen, a model poorly soluble drug.
27825861	3	65	theme	novel	759:763	arg1	strategy					771:778	a novel green strategy	757:778	a novel green strategy for the delivery of ibuprofen, a model poorly soluble drug	757:837	The focus of this study was to investigate the impacts of the in situ formation of polymer-drug nanoconjugate, at the interfacial nano-domains of two oppositely charged polymers, on the mechanism of film formation and to prepare aqueous ternary polymer-drug-polymer nanomatrix films as a novel green strategy for the delivery of ibuprofen, a model poorly soluble drug.
27825861	3	66	theme	in	533:534	arg1	formation					541:549	the in situ formation	529:549	the in situ formation of polymer-drug nanoconjugate	529:579	The focus of this study was to investigate the impacts of the in situ formation of polymer-drug nanoconjugate, at the interfacial nano-domains of two oppositely charged polymers, on the mechanism of film formation and to prepare aqueous ternary polymer-drug-polymer nanomatrix films as a novel green strategy for the delivery of ibuprofen, a model poorly soluble drug.
27825861	8	67	theme	higher	1488:1493	arg1	efficiency					1507:1516	higher dissolution efficiency	1488:1516	higher dissolution efficiency (99.76%)	1488:1525	The composite films exhibited reduced tensile strength and lower elastic modulus with optimal conjugation efficiency of 98.14±1.19%, which correlates with higher dissolution efficiency (99.76%) compared to 47.37% in layer-by-layer (LbL) films, dictated by Ddex concentration.
27825861	8	67	theme	higher	1488:1493	arg1	%					1524:1524	99.76%	1519:1524	99.76%	1519:1524	The composite films exhibited reduced tensile strength and lower elastic modulus with optimal conjugation efficiency of 98.14±1.19%, which correlates with higher dissolution efficiency (99.76%) compared to 47.37% in layer-by-layer (LbL) films, dictated by Ddex concentration.
27825861	10	68	theme	potential	1805:1813	arg1	application					1815:1825	the potential application	1801:1825	the potential application of aqueous drug-loaded nanomatrix films	1801:1865	This study demonstrated the potential application of aqueous drug-loaded nanomatrix films as controlled drug delivery strategy for ibuprofen, a model poorly soluble drug.
27825861	3	69	dep	in	533:534	arg1	situ					536:539	situ	536:539	situ	536:539	The focus of this study was to investigate the impacts of the in situ formation of polymer-drug nanoconjugate, at the interfacial nano-domains of two oppositely charged polymers, on the mechanism of film formation and to prepare aqueous ternary polymer-drug-polymer nanomatrix films as a novel green strategy for the delivery of ibuprofen, a model poorly soluble drug.
27825861	3	70	theme	soluble	826:832	arg1	drug					834:837	a model poorly soluble drug	811:837	a model poorly soluble drug	811:837	The focus of this study was to investigate the impacts of the in situ formation of polymer-drug nanoconjugate, at the interfacial nano-domains of two oppositely charged polymers, on the mechanism of film formation and to prepare aqueous ternary polymer-drug-polymer nanomatrix films as a novel green strategy for the delivery of ibuprofen, a model poorly soluble drug.
27825861	3	70	theme	soluble	826:832	arg1	ibuprofen					800:808	ibuprofen	800:808	ibuprofen	800:808	The focus of this study was to investigate the impacts of the in situ formation of polymer-drug nanoconjugate, at the interfacial nano-domains of two oppositely charged polymers, on the mechanism of film formation and to prepare aqueous ternary polymer-drug-polymer nanomatrix films as a novel green strategy for the delivery of ibuprofen, a model poorly soluble drug.
27825861	4	71	theme	self-assembly	961:973	arg1	concept					928:934	the concept	924:934	the concept of combined polymer-drug self-assembly and polyelectrolyte complexation	924:1006	Composite and Layer-by-Layer films were prepared by aqueous casting technique using the concept of combined polymer-drug self-assembly and polyelectrolyte complexation.
27825861	1	72	theme	polymeric	124:132	arg1	films					134:138	Aqueous polymeric films	116:138	Aqueous polymeric films	116:138	Aqueous polymeric films have potentially great values in drug development, particularly in controlled drug release and taste masking strategies.
27825861	4	73	theme	polyelectrolyte	979:993	arg1	complexation					995:1006	polyelectrolyte complexation	979:1006	polyelectrolyte complexation	979:1006	Composite and Layer-by-Layer films were prepared by aqueous casting technique using the concept of combined polymer-drug self-assembly and polyelectrolyte complexation.
27825861	3	74	theme	model	813:817	arg1	drug					834:837	a model poorly soluble drug	811:837	a model poorly soluble drug	811:837	The focus of this study was to investigate the impacts of the in situ formation of polymer-drug nanoconjugate, at the interfacial nano-domains of two oppositely charged polymers, on the mechanism of film formation and to prepare aqueous ternary polymer-drug-polymer nanomatrix films as a novel green strategy for the delivery of ibuprofen, a model poorly soluble drug.
27825861	3	74	theme	model	813:817	arg1	ibuprofen					800:808	ibuprofen	800:808	ibuprofen	800:808	The focus of this study was to investigate the impacts of the in situ formation of polymer-drug nanoconjugate, at the interfacial nano-domains of two oppositely charged polymers, on the mechanism of film formation and to prepare aqueous ternary polymer-drug-polymer nanomatrix films as a novel green strategy for the delivery of ibuprofen, a model poorly soluble drug.
27825861	7	75	theme	film	1320:1323	arg1	matrix					1325:1330	the film matrix	1316:1330	the film matrix	1316:1330	However in the presence of cationic dextran (Ddex), nanoconjugates (61.49±5.97-447.52±37.51nm) were formed within the core of the film matrix.
27825861	4	76	theme	combined	939:946	arg1	self-assembly					961:973	combined polymer-drug self-assembly	939:973	combined polymer-drug self-assembly	939:973	Composite and Layer-by-Layer films were prepared by aqueous casting technique using the concept of combined polymer-drug self-assembly and polyelectrolyte complexation.
27825861	8	77	theme	composite	1337:1345	arg1	films					1347:1351	The composite films	1333:1351	The composite films	1333:1351	The composite films exhibited reduced tensile strength and lower elastic modulus with optimal conjugation efficiency of 98.14±1.19%, which correlates with higher dissolution efficiency (99.76%) compared to 47.37% in layer-by-layer (LbL) films, dictated by Ddex concentration.
27825861	0	78	theme	ternary	23:29	arg1	films					42:46	aqueous ternary nanomatrix films	15:46	aqueous ternary nanomatrix films	15:46	Development of aqueous ternary nanomatrix films: A novel 'green' strategy for the delivery of poorly soluble drugs.
27825861	8	79	theme	layer-by-layer	1549:1562	arg1	films					1570:1574	layer-by-layer (LbL) films	1549:1574	layer-by-layer (LbL) films	1549:1574	The composite films exhibited reduced tensile strength and lower elastic modulus with optimal conjugation efficiency of 98.14±1.19%, which correlates with higher dissolution efficiency (99.76%) compared to 47.37% in layer-by-layer (LbL) films, dictated by Ddex concentration.
27825861	3	80	theme	charged	632:638	arg1	polymers					640:647	two oppositely charged polymers	617:647	two oppositely charged polymers	617:647	The focus of this study was to investigate the impacts of the in situ formation of polymer-drug nanoconjugate, at the interfacial nano-domains of two oppositely charged polymers, on the mechanism of film formation and to prepare aqueous ternary polymer-drug-polymer nanomatrix films as a novel green strategy for the delivery of ibuprofen, a model poorly soluble drug.
27825861	8	81	theme	tensile	1371:1377	arg1	strength					1379:1386	reduced tensile strength	1363:1386	reduced tensile strength	1363:1386	The composite films exhibited reduced tensile strength and lower elastic modulus with optimal conjugation efficiency of 98.14±1.19%, which correlates with higher dissolution efficiency (99.76%) compared to 47.37% in layer-by-layer (LbL) films, dictated by Ddex concentration.
27825861	2	82	theme	permeable	402:410	arg1	film					392:395	the film	388:395	the film less permeable	388:410	However the progressive polymer-particle coalescence that occurs randomly during film formation, curing and storage may render the film less permeable leading to erratic and unpredictable drug release profile.
27825861	0	83	theme	films	42:46	arg1	Development					0:10	Development	0:10	Development of aqueous ternary nanomatrix films: A novel 'green' strategy for the delivery of poorly soluble drugs.	0:114	Development of aqueous ternary nanomatrix films: A novel 'green' strategy for the delivery of poorly soluble drugs.
27825861	5	84	theme	drug-loaded	1023:1033	arg1	films					1046:1050	The plain and drug-loaded nanomatrix films	1009:1050	films	1046:1050	The plain and drug-loaded nanomatrix films were characterized using SEM, AFM, FTIR, DSC and TGA.
27825861	8	85	theme	LbL	1565:1567	arg1	films					1570:1574	layer-by-layer (LbL) films	1549:1574	layer-by-layer (LbL) films	1549:1574	The composite films exhibited reduced tensile strength and lower elastic modulus with optimal conjugation efficiency of 98.14±1.19%, which correlates with higher dissolution efficiency (99.76%) compared to 47.37% in layer-by-layer (LbL) films, dictated by Ddex concentration.
27825861	10	86	theme	drug-loaded	1838:1848	arg1	films					1861:1865	aqueous drug-loaded nanomatrix films	1830:1865	aqueous drug-loaded nanomatrix films	1830:1865	This study demonstrated the potential application of aqueous drug-loaded nanomatrix films as controlled drug delivery strategy for ibuprofen, a model poorly soluble drug.
27825861	9	87	theme	Ddex	1771:1774	arg1	concentration					1754:1766	higher concentration	1747:1766	higher concentration of Ddex	1747:1774	Generally, the mechanism of drug release was by Fickian diffusion, however anomalous transport or polymer relaxation was also observed at higher concentration of Ddex.
27825861	6	88	theme	core-shell	1133:1142	arg1	4.55-9.73μm					1161:1171	4.55-9.73μm	1161:1171	4.55-9.73μm	1161:1171	Ibuprofen formed spherical core-shell microstructures (4.55-9.73μm) in gellan film.
27825861	6	88	theme	core-shell	1133:1142	arg1	microstructures					1144:1158	spherical core-shell microstructures	1123:1158	spherical core-shell microstructures (4.55-9.73μm)	1123:1172	Ibuprofen formed spherical core-shell microstructures (4.55-9.73μm) in gellan film.
27825861	2	89	theme	drug	449:452	arg1	profile					462:468	erratic and unpredictable drug release profile	423:468	erratic and unpredictable drug release profile	423:468	However the progressive polymer-particle coalescence that occurs randomly during film formation, curing and storage may render the film less permeable leading to erratic and unpredictable drug release profile.
27825861	10	90	theme	delivery	1886:1893	arg1	strategy					1895:1902	controlled drug delivery strategy	1870:1902	controlled drug delivery strategy for ibuprofen, a model poorly soluble drug	1870:1945	This study demonstrated the potential application of aqueous drug-loaded nanomatrix films as controlled drug delivery strategy for ibuprofen, a model poorly soluble drug.
27825861	8	91	theme	elastic	1398:1404	arg1	modulus					1406:1412	lower elastic modulus	1392:1412	lower elastic modulus	1392:1412	The composite films exhibited reduced tensile strength and lower elastic modulus with optimal conjugation efficiency of 98.14±1.19%, which correlates with higher dissolution efficiency (99.76%) compared to 47.37% in layer-by-layer (LbL) films, dictated by Ddex concentration.
27825861	10	92	theme	controlled	1870:1879	arg1	strategy					1895:1902	controlled drug delivery strategy	1870:1902	controlled drug delivery strategy for ibuprofen, a model poorly soluble drug	1870:1945	This study demonstrated the potential application of aqueous drug-loaded nanomatrix films as controlled drug delivery strategy for ibuprofen, a model poorly soluble drug.
27825861	8	93	theme	Ddex	1589:1592	arg1	concentration					1594:1606	Ddex concentration	1589:1606	Ddex concentration	1589:1606	The composite films exhibited reduced tensile strength and lower elastic modulus with optimal conjugation efficiency of 98.14±1.19%, which correlates with higher dissolution efficiency (99.76%) compared to 47.37% in layer-by-layer (LbL) films, dictated by Ddex concentration.
27825861	3	94	theme	formation	675:683	arg1	mechanism					657:665	the mechanism	653:665	the mechanism of film formation	653:683	The focus of this study was to investigate the impacts of the in situ formation of polymer-drug nanoconjugate, at the interfacial nano-domains of two oppositely charged polymers, on the mechanism of film formation and to prepare aqueous ternary polymer-drug-polymer nanomatrix films as a novel green strategy for the delivery of ibuprofen, a model poorly soluble drug.
27825861	8	95	theme	conjugation	1427:1437	arg1	efficiency					1439:1448	optimal conjugation efficiency	1419:1448	optimal conjugation efficiency of 98.14±1.19%, which correlates with higher dissolution efficiency (99.76%)	1419:1525	The composite films exhibited reduced tensile strength and lower elastic modulus with optimal conjugation efficiency of 98.14±1.19%, which correlates with higher dissolution efficiency (99.76%) compared to 47.37% in layer-by-layer (LbL) films, dictated by Ddex concentration.
27825861	1	96	theme	drug	218:221	arg1	release					223:229	controlled drug release	207:229	controlled drug release	207:229	Aqueous polymeric films have potentially great values in drug development, particularly in controlled drug release and taste masking strategies.
26616450	10	0	theme	potential	1444:1452	arg1	applications					1454:1465	the potential applications	1440:1465	the potential applications of chitosan/soy protein-based biomaterials that may come into contact with blood	1440:1546	The improved cytocompatibility and hemocompatibility of HCS-n would shed light on the potential applications of chitosan/soy protein-based biomaterials that may come into contact with blood.
26616450	7	1	theme	recalcification	1014:1028	arg1	measurement					1041:1051	plasma recalcification time (PRT) measurement	1007:1051	plasma recalcification time (PRT) measurement	1007:1051	The cytocompatibility and hemocompatibility of CS-n and HCS-n were evaluated by cell culture (MTT assay, live/dead assay, cell morphology and cell density observation), platelet adhesion test, plasma recalcification time (PRT) measurement, hemolysis assay and thrombus formation test.
26616450	7	1	theme	recalcification	1014:1028	arg1	culture					899:905	cell culture	894:905	cell culture (MTT assay, live/dead assay, cell morphology and cell density observation)	894:980	The cytocompatibility and hemocompatibility of CS-n and HCS-n were evaluated by cell culture (MTT assay, live/dead assay, cell morphology and cell density observation), platelet adhesion test, plasma recalcification time (PRT) measurement, hemolysis assay and thrombus formation test.
26616450	9	2	dep	higher	1263:1268	arg1	BCI					1299:1301	BCI	1299:1301	BCI	1299:1301	HCS-n also exhibited lower platelet adhesion, longer PRT, higher blood anticoagulant indexes (BCI) and lower hemolysis rate than the corresponding CS-n.
26616450	9	2	dep	higher	1263:1268	arg1	indexes					1290:1296	blood anticoagulant indexes	1270:1296	blood anticoagulant indexes (BCI)	1270:1302	HCS-n also exhibited lower platelet adhesion, longer PRT, higher blood anticoagulant indexes (BCI) and lower hemolysis rate than the corresponding CS-n.
26616450	9	2	dep	higher	1263:1268	arg1	rate					1324:1327	lower hemolysis rate	1308:1327	lower hemolysis rate	1308:1327	HCS-n also exhibited lower platelet adhesion, longer PRT, higher blood anticoagulant indexes (BCI) and lower hemolysis rate than the corresponding CS-n.
26616450	1	3	theme	SPI	150:152	arg1	n=0					178:180	n=0	178:180	n=0	178:180	A series of chitosan/soy protein isolate (SPI) composite films (CS-n, n=0, 10 and 30, corresponding to SPI content in the composites) were prepared.
26616450	1	3	theme	SPI	150:152	arg1	CS-n					172:175	CS-n	172:175	CS-n	172:175	A series of chitosan/soy protein isolate (SPI) composite films (CS-n, n=0, 10 and 30, corresponding to SPI content in the composites) were prepared.
26616450	1	3	theme	SPI	150:152	arg1	films					165:169	chitosan/soy protein isolate (SPI) composite films	120:169	chitosan/soy protein isolate (SPI) composite films (CS-n, n=0, 10 and 30, corresponding to SPI content in the composites)	120:240	A series of chitosan/soy protein isolate (SPI) composite films (CS-n, n=0, 10 and 30, corresponding to SPI content in the composites) were prepared.
26616450	7	4	dep	cytocompatibility	818:834	arg1	The					814:816	The	814:816	The	814:816	The cytocompatibility and hemocompatibility of CS-n and HCS-n were evaluated by cell culture (MTT assay, live/dead assay, cell morphology and cell density observation), platelet adhesion test, plasma recalcification time (PRT) measurement, hemolysis assay and thrombus formation test.
26616450	7	5	theme	time	1030:1033	arg1	measurement					1041:1051	plasma recalcification time (PRT) measurement	1007:1051	plasma recalcification time (PRT) measurement	1007:1051	The cytocompatibility and hemocompatibility of CS-n and HCS-n were evaluated by cell culture (MTT assay, live/dead assay, cell morphology and cell density observation), platelet adhesion test, plasma recalcification time (PRT) measurement, hemolysis assay and thrombus formation test.
26616450	7	5	theme	time	1030:1033	arg1	culture					899:905	cell culture	894:905	cell culture (MTT assay, live/dead assay, cell morphology and cell density observation)	894:980	The cytocompatibility and hemocompatibility of CS-n and HCS-n were evaluated by cell culture (MTT assay, live/dead assay, cell morphology and cell density observation), platelet adhesion test, plasma recalcification time (PRT) measurement, hemolysis assay and thrombus formation test.
26616450	8	6	theme	higher	1112:1117	arg1	rate					1133:1136	higher cell adhesion rate	1112:1136	higher cell adhesion rate	1112:1136	HCS-n showed higher cell adhesion rate and improved cytocompatibility compared to the corresponding CS-n.
26616450	3	7	theme	contact	445:451	arg1	angle					453:457	water contact angle	439:457	water contact angle	439:457	CS-n and HCS-n were characterized by ATR-Fourier transform infrared spectroscopy and water contact angle.
26616450	10	8	theme	chitosan/soy	1470:1481	arg1	biomaterials					1497:1508	chitosan/soy protein-based biomaterials	1470:1508	chitosan/soy protein-based biomaterials that may come into contact with blood	1470:1546	The improved cytocompatibility and hemocompatibility of HCS-n would shed light on the potential applications of chitosan/soy protein-based biomaterials that may come into contact with blood.
26616450	1	9	theme	SPI	211:213	arg1	content					215:221	SPI content	211:221	SPI content in the composites	211:239	A series of chitosan/soy protein isolate (SPI) composite films (CS-n, n=0, 10 and 30, corresponding to SPI content in the composites) were prepared.
26616450	0	10	from	Improvement	0:10	arg1	properties					39:48	physical and biological properties	15:48	physical and biological properties of chitosan/soy protein films	15:78	Improvement in physical and biological properties of chitosan/soy protein films by surface grafted heparin.
26616450	7	11	theme	HCS-n	870:874	arg1	hemocompatibility					840:856	hemocompatibility	840:856	hemocompatibility	840:856	The cytocompatibility and hemocompatibility of CS-n and HCS-n were evaluated by cell culture (MTT assay, live/dead assay, cell morphology and cell density observation), platelet adhesion test, plasma recalcification time (PRT) measurement, hemolysis assay and thrombus formation test.
26616450	7	11	theme	HCS-n	870:874	arg1	cytocompatibility					818:834	cytocompatibility	818:834	cytocompatibility	818:834	The cytocompatibility and hemocompatibility of CS-n and HCS-n were evaluated by cell culture (MTT assay, live/dead assay, cell morphology and cell density observation), platelet adhesion test, plasma recalcification time (PRT) measurement, hemolysis assay and thrombus formation test.
26616450	3	12	dep	transform	403:411	arg1	infrared					413:420	infrared	413:420	transform infrared spectroscopy and water contact angle	403:457	CS-n and HCS-n were characterized by ATR-Fourier transform infrared spectroscopy and water contact angle.
26616450	9	13	theme	anticoagulant	1276:1288	arg1	BCI					1299:1301	BCI	1299:1301	BCI	1299:1301	HCS-n also exhibited lower platelet adhesion, longer PRT, higher blood anticoagulant indexes (BCI) and lower hemolysis rate than the corresponding CS-n.
26616450	9	13	theme	anticoagulant	1276:1288	arg1	indexes					1290:1296	blood anticoagulant indexes	1270:1296	blood anticoagulant indexes (BCI)	1270:1302	HCS-n also exhibited lower platelet adhesion, longer PRT, higher blood anticoagulant indexes (BCI) and lower hemolysis rate than the corresponding CS-n.
26616450	9	14	theme	hemolysis	1314:1322	arg1	rate					1324:1327	lower hemolysis rate	1308:1327	lower hemolysis rate	1308:1327	HCS-n also exhibited lower platelet adhesion, longer PRT, higher blood anticoagulant indexes (BCI) and lower hemolysis rate than the corresponding CS-n.
26616450	10	15	theme	biomaterials	1497:1508	arg1	applications					1454:1465	the potential applications	1440:1465	the potential applications of chitosan/soy protein-based biomaterials that may come into contact with blood	1440:1546	The improved cytocompatibility and hemocompatibility of HCS-n would shed light on the potential applications of chitosan/soy protein-based biomaterials that may come into contact with blood.
26616450	7	16	theme	plasma	1007:1012	arg1	measurement					1041:1051	plasma recalcification time (PRT) measurement	1007:1051	plasma recalcification time (PRT) measurement	1007:1051	The cytocompatibility and hemocompatibility of CS-n and HCS-n were evaluated by cell culture (MTT assay, live/dead assay, cell morphology and cell density observation), platelet adhesion test, plasma recalcification time (PRT) measurement, hemolysis assay and thrombus formation test.
26616450	7	16	theme	plasma	1007:1012	arg1	culture					899:905	cell culture	894:905	cell culture (MTT assay, live/dead assay, cell morphology and cell density observation)	894:980	The cytocompatibility and hemocompatibility of CS-n and HCS-n were evaluated by cell culture (MTT assay, live/dead assay, cell morphology and cell density observation), platelet adhesion test, plasma recalcification time (PRT) measurement, hemolysis assay and thrombus formation test.
26616450	6	17	theme	content	728:734	arg1	increase					712:719	the increase	708:719	the increase of SPI content	708:734	Heparin evenly distributed on the surface of the films and the heparin content increased with the increase of SPI content, and the hydrophilicity of the films was enhanced due to the grafted heparin.
26616450	2	18	theme	CS-n	297:300	arg1	surface					286:292	the surface	282:292	the surface of CS-n	282:300	Heparin was grafted onto the surface of CS-n to fabricate a series of heparinized films (HCS-n).
26616450	7	19	dep	assay	912:916	arg1	observation					969:979	observation	969:979	observation	969:979	The cytocompatibility and hemocompatibility of CS-n and HCS-n were evaluated by cell culture (MTT assay, live/dead assay, cell morphology and cell density observation), platelet adhesion test, plasma recalcification time (PRT) measurement, hemolysis assay and thrombus formation test.
26616450	7	20	theme	thrombus	1074:1081	arg1	formation					1083:1091	thrombus formation	1074:1091	thrombus formation test	1074:1096	The cytocompatibility and hemocompatibility of CS-n and HCS-n were evaluated by cell culture (MTT assay, live/dead assay, cell morphology and cell density observation), platelet adhesion test, plasma recalcification time (PRT) measurement, hemolysis assay and thrombus formation test.
26616450	6	21	theme	grafted	797:803	arg1	heparin					805:811	the grafted heparin	793:811	the grafted heparin	793:811	Heparin evenly distributed on the surface of the films and the heparin content increased with the increase of SPI content, and the hydrophilicity of the films was enhanced due to the grafted heparin.
26616450	7	22	theme	adhesion	992:999	arg1	test					1001:1004	platelet adhesion test	983:1004	platelet adhesion test	983:1004	The cytocompatibility and hemocompatibility of CS-n and HCS-n were evaluated by cell culture (MTT assay, live/dead assay, cell morphology and cell density observation), platelet adhesion test, plasma recalcification time (PRT) measurement, hemolysis assay and thrombus formation test.
26616450	7	22	theme	adhesion	992:999	arg1	culture					899:905	cell culture	894:905	cell culture (MTT assay, live/dead assay, cell morphology and cell density observation)	894:980	The cytocompatibility and hemocompatibility of CS-n and HCS-n were evaluated by cell culture (MTT assay, live/dead assay, cell morphology and cell density observation), platelet adhesion test, plasma recalcification time (PRT) measurement, hemolysis assay and thrombus formation test.
26616450	6	23	theme	SPI	724:726	arg1	content					728:734	SPI content	724:734	SPI content	724:734	Heparin evenly distributed on the surface of the films and the heparin content increased with the increase of SPI content, and the hydrophilicity of the films was enhanced due to the grafted heparin.
26616450	1	24	theme	composite	155:163	arg1	n=0					178:180	n=0	178:180	n=0	178:180	A series of chitosan/soy protein isolate (SPI) composite films (CS-n, n=0, 10 and 30, corresponding to SPI content in the composites) were prepared.
26616450	1	24	theme	composite	155:163	arg1	CS-n					172:175	CS-n	172:175	CS-n	172:175	A series of chitosan/soy protein isolate (SPI) composite films (CS-n, n=0, 10 and 30, corresponding to SPI content in the composites) were prepared.
26616450	1	24	theme	composite	155:163	arg1	films					165:169	chitosan/soy protein isolate (SPI) composite films	120:169	chitosan/soy protein isolate (SPI) composite films (CS-n, n=0, 10 and 30, corresponding to SPI content in the composites)	120:240	A series of chitosan/soy protein isolate (SPI) composite films (CS-n, n=0, 10 and 30, corresponding to SPI content in the composites) were prepared.
26616450	7	25	theme	formation	1083:1091	arg1	test					1093:1096	thrombus formation test	1074:1096	thrombus formation test	1074:1096	The cytocompatibility and hemocompatibility of CS-n and HCS-n were evaluated by cell culture (MTT assay, live/dead assay, cell morphology and cell density observation), platelet adhesion test, plasma recalcification time (PRT) measurement, hemolysis assay and thrombus formation test.
26616450	7	25	theme	formation	1083:1091	arg1	culture					899:905	cell culture	894:905	cell culture (MTT assay, live/dead assay, cell morphology and cell density observation)	894:980	The cytocompatibility and hemocompatibility of CS-n and HCS-n were evaluated by cell culture (MTT assay, live/dead assay, cell morphology and cell density observation), platelet adhesion test, plasma recalcification time (PRT) measurement, hemolysis assay and thrombus formation test.
26616450	9	26	theme	lower	1226:1230	arg1	adhesion					1241:1248	lower platelet adhesion	1226:1248	lower platelet adhesion	1226:1248	HCS-n also exhibited lower platelet adhesion, longer PRT, higher blood anticoagulant indexes (BCI) and lower hemolysis rate than the corresponding CS-n.
26616450	10	27	theme	improved	1362:1369	arg1	cytocompatibility					1371:1387	improved cytocompatibility	1362:1387	improved cytocompatibility	1362:1387	The improved cytocompatibility and hemocompatibility of HCS-n would shed light on the potential applications of chitosan/soy protein-based biomaterials that may come into contact with blood.
26616450	7	28	theme	CS-n	861:864	arg1	hemocompatibility					840:856	hemocompatibility	840:856	hemocompatibility	840:856	The cytocompatibility and hemocompatibility of CS-n and HCS-n were evaluated by cell culture (MTT assay, live/dead assay, cell morphology and cell density observation), platelet adhesion test, plasma recalcification time (PRT) measurement, hemolysis assay and thrombus formation test.
26616450	7	28	theme	CS-n	861:864	arg1	cytocompatibility					818:834	cytocompatibility	818:834	cytocompatibility	818:834	The cytocompatibility and hemocompatibility of CS-n and HCS-n were evaluated by cell culture (MTT assay, live/dead assay, cell morphology and cell density observation), platelet adhesion test, plasma recalcification time (PRT) measurement, hemolysis assay and thrombus formation test.
26616450	7	29	theme	MTT	908:910	arg1	assay					912:916	MTT assay	908:916	MTT assay	908:916	The cytocompatibility and hemocompatibility of CS-n and HCS-n were evaluated by cell culture (MTT assay, live/dead assay, cell morphology and cell density observation), platelet adhesion test, plasma recalcification time (PRT) measurement, hemolysis assay and thrombus formation test.
26616450	1	30	theme	films	165:169	arg1	series					110:115	A series	108:115	A series of chitosan/soy protein isolate (SPI) composite films (CS-n, n=0, 10 and 30, corresponding to SPI content in the composites)	108:240	A series of chitosan/soy protein isolate (SPI) composite films (CS-n, n=0, 10 and 30, corresponding to SPI content in the composites) were prepared.
26616450	9	31	theme	platelet	1232:1239	arg1	adhesion					1241:1248	lower platelet adhesion	1226:1248	lower platelet adhesion	1226:1248	HCS-n also exhibited lower platelet adhesion, longer PRT, higher blood anticoagulant indexes (BCI) and lower hemolysis rate than the corresponding CS-n.
26616450	9	32	theme	corresponding	1338:1350	arg1	CS-n					1352:1355	the corresponding CS-n	1334:1355	the corresponding CS-n	1334:1355	HCS-n also exhibited lower platelet adhesion, longer PRT, higher blood anticoagulant indexes (BCI) and lower hemolysis rate than the corresponding CS-n.
26616450	7	33	theme	platelet	983:990	arg1	test					1001:1004	platelet adhesion test	983:1004	platelet adhesion test	983:1004	The cytocompatibility and hemocompatibility of CS-n and HCS-n were evaluated by cell culture (MTT assay, live/dead assay, cell morphology and cell density observation), platelet adhesion test, plasma recalcification time (PRT) measurement, hemolysis assay and thrombus formation test.
26616450	7	33	theme	platelet	983:990	arg1	culture					899:905	cell culture	894:905	cell culture (MTT assay, live/dead assay, cell morphology and cell density observation)	894:980	The cytocompatibility and hemocompatibility of CS-n and HCS-n were evaluated by cell culture (MTT assay, live/dead assay, cell morphology and cell density observation), platelet adhesion test, plasma recalcification time (PRT) measurement, hemolysis assay and thrombus formation test.
26616450	10	34	dep	come	1519:1522	arg1	may					1515:1517	may	1515:1517	may	1515:1517	The improved cytocompatibility and hemocompatibility of HCS-n would shed light on the potential applications of chitosan/soy protein-based biomaterials that may come into contact with blood.
26616450	10	35	dep	cytocompatibility	1371:1387	arg1	The					1358:1360	The	1358:1360	The	1358:1360	The improved cytocompatibility and hemocompatibility of HCS-n would shed light on the potential applications of chitosan/soy protein-based biomaterials that may come into contact with blood.
26616450	1	36	from	content	215:221	arg1	composites					230:239	the composites	226:239	the composites	226:239	A series of chitosan/soy protein isolate (SPI) composite films (CS-n, n=0, 10 and 30, corresponding to SPI content in the composites) were prepared.
26616450	7	37	dep	culture	899:905	arg1	morphology					941:950	cell morphology	936:950	cell morphology	936:950	The cytocompatibility and hemocompatibility of CS-n and HCS-n were evaluated by cell culture (MTT assay, live/dead assay, cell morphology and cell density observation), platelet adhesion test, plasma recalcification time (PRT) measurement, hemolysis assay and thrombus formation test.
26616450	7	37	dep	culture	899:905	arg1	density					961:967	cell density	956:967	cell density	956:967	The cytocompatibility and hemocompatibility of CS-n and HCS-n were evaluated by cell culture (MTT assay, live/dead assay, cell morphology and cell density observation), platelet adhesion test, plasma recalcification time (PRT) measurement, hemolysis assay and thrombus formation test.
26616450	7	37	dep	culture	899:905	arg1	assay					912:916	MTT assay	908:916	MTT assay	908:916	The cytocompatibility and hemocompatibility of CS-n and HCS-n were evaluated by cell culture (MTT assay, live/dead assay, cell morphology and cell density observation), platelet adhesion test, plasma recalcification time (PRT) measurement, hemolysis assay and thrombus formation test.
26616450	7	37	dep	culture	899:905	arg1	assay					929:933	live/dead assay	919:933	live/dead assay	919:933	The cytocompatibility and hemocompatibility of CS-n and HCS-n were evaluated by cell culture (MTT assay, live/dead assay, cell morphology and cell density observation), platelet adhesion test, plasma recalcification time (PRT) measurement, hemolysis assay and thrombus formation test.
26616450	0	38	theme	physical	15:22	arg1	properties					39:48	physical and biological properties	15:48	physical and biological properties of chitosan/soy protein films	15:78	Improvement in physical and biological properties of chitosan/soy protein films by surface grafted heparin.
26616450	10	39	with	contact	1529:1535	arg1	blood					1542:1546	blood	1542:1546	blood	1542:1546	The improved cytocompatibility and hemocompatibility of HCS-n would shed light on the potential applications of chitosan/soy protein-based biomaterials that may come into contact with blood.
26616450	4	40	theme	blue	514:517	arg1	assay					519:523	toluidine blue assay	504:523	toluidine blue assay	504:523	The surface heparin density was measured by toluidine blue assay.
26616450	3	41	theme	water	439:443	arg1	angle					453:457	water contact angle	439:457	water contact angle	439:457	CS-n and HCS-n were characterized by ATR-Fourier transform infrared spectroscopy and water contact angle.
26616450	7	42	theme	cell	956:959	arg1	density					961:967	cell density	956:967	cell density	956:967	The cytocompatibility and hemocompatibility of CS-n and HCS-n were evaluated by cell culture (MTT assay, live/dead assay, cell morphology and cell density observation), platelet adhesion test, plasma recalcification time (PRT) measurement, hemolysis assay and thrombus formation test.
26616450	2	43	theme	heparinized	327:337	arg1	HCS-n					346:350	HCS-n	346:350	HCS-n	346:350	Heparin was grafted onto the surface of CS-n to fabricate a series of heparinized films (HCS-n).
26616450	2	43	theme	heparinized	327:337	arg1	films					339:343	heparinized films	327:343	heparinized films (HCS-n)	327:351	Heparin was grafted onto the surface of CS-n to fabricate a series of heparinized films (HCS-n).
26616450	4	44	theme	surface	464:470	arg1	density					480:486	The surface heparin density	460:486	The surface heparin density	460:486	The surface heparin density was measured by toluidine blue assay.
26616450	0	45	theme	biological	28:37	arg1	properties					39:48	physical and biological properties	15:48	physical and biological properties of chitosan/soy protein films	15:78	Improvement in physical and biological properties of chitosan/soy protein films by surface grafted heparin.
26616450	7	46	theme	live/dead	919:927	arg1	assay					929:933	live/dead assay	919:933	live/dead assay	919:933	The cytocompatibility and hemocompatibility of CS-n and HCS-n were evaluated by cell culture (MTT assay, live/dead assay, cell morphology and cell density observation), platelet adhesion test, plasma recalcification time (PRT) measurement, hemolysis assay and thrombus formation test.
26616450	2	47	theme	films	339:343	arg1	series					317:322	a series	315:322	a series of heparinized films (HCS-n)	315:351	Heparin was grafted onto the surface of CS-n to fabricate a series of heparinized films (HCS-n).
26616450	0	48	theme	chitosan/soy	53:64	arg1	films					74:78	chitosan/soy protein films	53:78	chitosan/soy protein films	53:78	Improvement in physical and biological properties of chitosan/soy protein films by surface grafted heparin.
26616450	5	49	theme	CS-n	608:611	arg1	surface					597:603	the surface	593:603	the surface of CS-n	593:611	The results showed that heparin has been successfully grafted onto the surface of CS-n.
26616450	6	50	theme	films	767:771	arg1	hydrophilicity					745:758	the hydrophilicity	741:758	the hydrophilicity of the films	741:771	Heparin evenly distributed on the surface of the films and the heparin content increased with the increase of SPI content, and the hydrophilicity of the films was enhanced due to the grafted heparin.
26616450	7	51	theme	PRT	1036:1038	arg1	measurement					1041:1051	plasma recalcification time (PRT) measurement	1007:1051	plasma recalcification time (PRT) measurement	1007:1051	The cytocompatibility and hemocompatibility of CS-n and HCS-n were evaluated by cell culture (MTT assay, live/dead assay, cell morphology and cell density observation), platelet adhesion test, plasma recalcification time (PRT) measurement, hemolysis assay and thrombus formation test.
26616450	7	51	theme	PRT	1036:1038	arg1	culture					899:905	cell culture	894:905	cell culture (MTT assay, live/dead assay, cell morphology and cell density observation)	894:980	The cytocompatibility and hemocompatibility of CS-n and HCS-n were evaluated by cell culture (MTT assay, live/dead assay, cell morphology and cell density observation), platelet adhesion test, plasma recalcification time (PRT) measurement, hemolysis assay and thrombus formation test.
26616450	9	52	theme	blood	1270:1274	arg1	BCI					1299:1301	BCI	1299:1301	BCI	1299:1301	HCS-n also exhibited lower platelet adhesion, longer PRT, higher blood anticoagulant indexes (BCI) and lower hemolysis rate than the corresponding CS-n.
26616450	9	52	theme	blood	1270:1274	arg1	indexes					1290:1296	blood anticoagulant indexes	1270:1296	blood anticoagulant indexes (BCI)	1270:1302	HCS-n also exhibited lower platelet adhesion, longer PRT, higher blood anticoagulant indexes (BCI) and lower hemolysis rate than the corresponding CS-n.
26616450	10	53	theme	HCS-n	1414:1418	arg1	hemocompatibility					1393:1409	hemocompatibility	1393:1409	hemocompatibility	1393:1409	The improved cytocompatibility and hemocompatibility of HCS-n would shed light on the potential applications of chitosan/soy protein-based biomaterials that may come into contact with blood.
26616450	10	53	theme	HCS-n	1414:1418	arg1	cytocompatibility					1371:1387	improved cytocompatibility	1362:1387	improved cytocompatibility	1362:1387	The improved cytocompatibility and hemocompatibility of HCS-n would shed light on the potential applications of chitosan/soy protein-based biomaterials that may come into contact with blood.
26616450	1	54	dep	films	165:169	arg1	n=0					178:180	n=0	178:180	n=0	178:180	A series of chitosan/soy protein isolate (SPI) composite films (CS-n, n=0, 10 and 30, corresponding to SPI content in the composites) were prepared.
26616450	1	54	dep	films	165:169	arg1	CS-n					172:175	CS-n	172:175	CS-n	172:175	A series of chitosan/soy protein isolate (SPI) composite films (CS-n, n=0, 10 and 30, corresponding to SPI content in the composites) were prepared.
26616450	1	54	dep	films	165:169	arg1	films					165:169	chitosan/soy protein isolate (SPI) composite films	120:169	chitosan/soy protein isolate (SPI) composite films (CS-n, n=0, 10 and 30, corresponding to SPI content in the composites)	120:240	A series of chitosan/soy protein isolate (SPI) composite films (CS-n, n=0, 10 and 30, corresponding to SPI content in the composites) were prepared.
26616450	1	54	dep	films	165:169	arg1	30					190:191	30	190:191	30	190:191	A series of chitosan/soy protein isolate (SPI) composite films (CS-n, n=0, 10 and 30, corresponding to SPI content in the composites) were prepared.
26616450	1	54	dep	films	165:169	arg1	10					183:184	10	183:184	10	183:184	A series of chitosan/soy protein isolate (SPI) composite films (CS-n, n=0, 10 and 30, corresponding to SPI content in the composites) were prepared.
26616450	6	55	theme	heparin	677:683	arg1	content					685:691	the heparin content	673:691	the heparin content	673:691	Heparin evenly distributed on the surface of the films and the heparin content increased with the increase of SPI content, and the hydrophilicity of the films was enhanced due to the grafted heparin.
26616450	8	56	theme	corresponding	1185:1197	arg1	CS-n					1199:1202	the corresponding CS-n	1181:1202	the corresponding CS-n	1181:1202	HCS-n showed higher cell adhesion rate and improved cytocompatibility compared to the corresponding CS-n.
26616450	4	57	theme	heparin	472:478	arg1	density					480:486	The surface heparin density	460:486	The surface heparin density	460:486	The surface heparin density was measured by toluidine blue assay.
26616450	1	58	theme	chitosan/soy	120:131	arg1	n=0					178:180	n=0	178:180	n=0	178:180	A series of chitosan/soy protein isolate (SPI) composite films (CS-n, n=0, 10 and 30, corresponding to SPI content in the composites) were prepared.
26616450	1	58	theme	chitosan/soy	120:131	arg1	CS-n					172:175	CS-n	172:175	CS-n	172:175	A series of chitosan/soy protein isolate (SPI) composite films (CS-n, n=0, 10 and 30, corresponding to SPI content in the composites) were prepared.
26616450	1	58	theme	chitosan/soy	120:131	arg1	films					165:169	chitosan/soy protein isolate (SPI) composite films	120:169	chitosan/soy protein isolate (SPI) composite films (CS-n, n=0, 10 and 30, corresponding to SPI content in the composites)	120:240	A series of chitosan/soy protein isolate (SPI) composite films (CS-n, n=0, 10 and 30, corresponding to SPI content in the composites) were prepared.
26616450	10	59	theme	protein-based	1483:1495	arg1	biomaterials					1497:1508	chitosan/soy protein-based biomaterials	1470:1508	chitosan/soy protein-based biomaterials that may come into contact with blood	1470:1546	The improved cytocompatibility and hemocompatibility of HCS-n would shed light on the potential applications of chitosan/soy protein-based biomaterials that may come into contact with blood.
26616450	7	60	theme	cell	936:939	arg1	morphology					941:950	cell morphology	936:950	cell morphology	936:950	The cytocompatibility and hemocompatibility of CS-n and HCS-n were evaluated by cell culture (MTT assay, live/dead assay, cell morphology and cell density observation), platelet adhesion test, plasma recalcification time (PRT) measurement, hemolysis assay and thrombus formation test.
26616450	9	61	theme	lower	1308:1312	arg1	rate					1324:1327	lower hemolysis rate	1308:1327	lower hemolysis rate	1308:1327	HCS-n also exhibited lower platelet adhesion, longer PRT, higher blood anticoagulant indexes (BCI) and lower hemolysis rate than the corresponding CS-n.
26616450	1	62	theme	protein	133:139	arg1	n=0					178:180	n=0	178:180	n=0	178:180	A series of chitosan/soy protein isolate (SPI) composite films (CS-n, n=0, 10 and 30, corresponding to SPI content in the composites) were prepared.
26616450	1	62	theme	protein	133:139	arg1	CS-n					172:175	CS-n	172:175	CS-n	172:175	A series of chitosan/soy protein isolate (SPI) composite films (CS-n, n=0, 10 and 30, corresponding to SPI content in the composites) were prepared.
26616450	1	62	theme	protein	133:139	arg1	films					165:169	chitosan/soy protein isolate (SPI) composite films	120:169	chitosan/soy protein isolate (SPI) composite films (CS-n, n=0, 10 and 30, corresponding to SPI content in the composites)	120:240	A series of chitosan/soy protein isolate (SPI) composite films (CS-n, n=0, 10 and 30, corresponding to SPI content in the composites) were prepared.
26616450	8	63	theme	cell	1119:1122	arg1	rate					1133:1136	higher cell adhesion rate	1112:1136	higher cell adhesion rate	1112:1136	HCS-n showed higher cell adhesion rate and improved cytocompatibility compared to the corresponding CS-n.
26616450	0	64	theme	films	74:78	arg1	properties					39:48	physical and biological properties	15:48	physical and biological properties of chitosan/soy protein films	15:78	Improvement in physical and biological properties of chitosan/soy protein films by surface grafted heparin.
26616450	6	65	theme	films	663:667	arg1	surface					648:654	the surface	644:654	the surface of the films	644:667	Heparin evenly distributed on the surface of the films and the heparin content increased with the increase of SPI content, and the hydrophilicity of the films was enhanced due to the grafted heparin.
26616450	1	66	theme	isolate	141:147	arg1	n=0					178:180	n=0	178:180	n=0	178:180	A series of chitosan/soy protein isolate (SPI) composite films (CS-n, n=0, 10 and 30, corresponding to SPI content in the composites) were prepared.
26616450	1	66	theme	isolate	141:147	arg1	CS-n					172:175	CS-n	172:175	CS-n	172:175	A series of chitosan/soy protein isolate (SPI) composite films (CS-n, n=0, 10 and 30, corresponding to SPI content in the composites) were prepared.
26616450	1	66	theme	isolate	141:147	arg1	films					165:169	chitosan/soy protein isolate (SPI) composite films	120:169	chitosan/soy protein isolate (SPI) composite films (CS-n, n=0, 10 and 30, corresponding to SPI content in the composites)	120:240	A series of chitosan/soy protein isolate (SPI) composite films (CS-n, n=0, 10 and 30, corresponding to SPI content in the composites) were prepared.
26616450	8	67	theme	adhesion	1124:1131	arg1	rate					1133:1136	higher cell adhesion rate	1112:1136	higher cell adhesion rate	1112:1136	HCS-n showed higher cell adhesion rate and improved cytocompatibility compared to the corresponding CS-n.
26616450	0	68	theme	protein	66:72	arg1	films					74:78	chitosan/soy protein films	53:78	chitosan/soy protein films	53:78	Improvement in physical and biological properties of chitosan/soy protein films by surface grafted heparin.
26616450	3	69	dep	ATR-Fourier	391:401	arg1	transform					403:411	transform	403:411	transform infrared spectroscopy and water contact angle	403:457	CS-n and HCS-n were characterized by ATR-Fourier transform infrared spectroscopy and water contact angle.
26616450	7	70	theme	cell	894:897	arg1	test					1093:1096	thrombus formation test	1074:1096	thrombus formation test	1074:1096	The cytocompatibility and hemocompatibility of CS-n and HCS-n were evaluated by cell culture (MTT assay, live/dead assay, cell morphology and cell density observation), platelet adhesion test, plasma recalcification time (PRT) measurement, hemolysis assay and thrombus formation test.
26616450	7	70	theme	cell	894:897	arg1	measurement					1041:1051	plasma recalcification time (PRT) measurement	1007:1051	plasma recalcification time (PRT) measurement	1007:1051	The cytocompatibility and hemocompatibility of CS-n and HCS-n were evaluated by cell culture (MTT assay, live/dead assay, cell morphology and cell density observation), platelet adhesion test, plasma recalcification time (PRT) measurement, hemolysis assay and thrombus formation test.
26616450	7	70	theme	cell	894:897	arg1	assay					1064:1068	hemolysis assay	1054:1068	hemolysis assay	1054:1068	The cytocompatibility and hemocompatibility of CS-n and HCS-n were evaluated by cell culture (MTT assay, live/dead assay, cell morphology and cell density observation), platelet adhesion test, plasma recalcification time (PRT) measurement, hemolysis assay and thrombus formation test.
26616450	7	70	theme	cell	894:897	arg1	culture					899:905	cell culture	894:905	cell culture (MTT assay, live/dead assay, cell morphology and cell density observation)	894:980	The cytocompatibility and hemocompatibility of CS-n and HCS-n were evaluated by cell culture (MTT assay, live/dead assay, cell morphology and cell density observation), platelet adhesion test, plasma recalcification time (PRT) measurement, hemolysis assay and thrombus formation test.
26616450	7	70	theme	cell	894:897	arg1	test					1001:1004	platelet adhesion test	983:1004	platelet adhesion test	983:1004	The cytocompatibility and hemocompatibility of CS-n and HCS-n were evaluated by cell culture (MTT assay, live/dead assay, cell morphology and cell density observation), platelet adhesion test, plasma recalcification time (PRT) measurement, hemolysis assay and thrombus formation test.
26616450	7	71	theme	hemolysis	1054:1062	arg1	assay					1064:1068	hemolysis assay	1054:1068	hemolysis assay	1054:1068	The cytocompatibility and hemocompatibility of CS-n and HCS-n were evaluated by cell culture (MTT assay, live/dead assay, cell morphology and cell density observation), platelet adhesion test, plasma recalcification time (PRT) measurement, hemolysis assay and thrombus formation test.
26616450	7	71	theme	hemolysis	1054:1062	arg1	culture					899:905	cell culture	894:905	cell culture (MTT assay, live/dead assay, cell morphology and cell density observation)	894:980	The cytocompatibility and hemocompatibility of CS-n and HCS-n were evaluated by cell culture (MTT assay, live/dead assay, cell morphology and cell density observation), platelet adhesion test, plasma recalcification time (PRT) measurement, hemolysis assay and thrombus formation test.
27416015	3	0	theme	NMR	300:302	arg1	spectroscopy					304:315	(13)C NMR spectroscopy	294:315	(13)C NMR spectroscopy	294:315	chlororaphis UCM B-106 and studied by composition analysis along with 1D and 2D (1)H and (13)C NMR spectroscopy.
27416015	4	1	theme	1→	530:531	arg1	acid					654:657	the following structure of the trisaccharide repeating unit was established: →4)-β-Psep5Ac7Hb-(2 → 6)-β-d-Galf-(1 → 3)-β-d-Galp-(1→ where Pse5Ac7Hb indicates 5-acetamido-3,5,7,9-tetradeoxy-7-[(R)-3-hydroxybutanoylamino]-l-glycero-l-manno-non-2-ulosonic acid	401:657	the following structure of the trisaccharide repeating unit was established: →4)-β-Psep5Ac7Hb-(2 → 6)-β-d-Galf-(1 → 3)-β-d-Galp-(1→ where Pse5Ac7Hb indicates 5-acetamido-3,5,7,9-tetradeoxy-7-[(R)-3-hydroxybutanoylamino]-l-glycero-l-manno-non-2-ulosonic acid	401:657	The polysaccharide was found to contain a derivative of pseudaminic acid (Pse) and the following structure of the trisaccharide repeating unit was established: →4)-β-Psep5Ac7Hb-(2 → 6)-β-d-Galf-(1 → 3)-β-d-Galp-(1→ where Pse5Ac7Hb indicates 5-acetamido-3,5,7,9-tetradeoxy-7-[(R)-3-hydroxybutanoylamino]-l-glycero-l-manno-non-2-ulosonic acid.
27416015	3	2	theme	chlororaphis	205:216	arg1	B-106					222:226	chlororaphis UCM B-106	205:226	chlororaphis UCM B-106	205:226	chlororaphis UCM B-106 and studied by composition analysis along with 1D and 2D (1)H and (13)C NMR spectroscopy.
27416015	3	3	theme	composition	243:253	arg1	analysis					255:262	composition analysis	243:262	composition analysis	243:262	chlororaphis UCM B-106 and studied by composition analysis along with 1D and 2D (1)H and (13)C NMR spectroscopy.
27416015	3	4	theme	UCM	218:220	arg1	B-106					222:226	chlororaphis UCM B-106	205:226	chlororaphis UCM B-106	205:226	chlororaphis UCM B-106 and studied by composition analysis along with 1D and 2D (1)H and (13)C NMR spectroscopy.
27416015	4	5	dep	structure	415:423	arg1	established					465:475	established	465:475	was established	461:475	The polysaccharide was found to contain a derivative of pseudaminic acid (Pse) and the following structure of the trisaccharide repeating unit was established: →4)-β-Psep5Ac7Hb-(2 → 6)-β-d-Galf-(1 → 3)-β-d-Galp-(1→ where Pse5Ac7Hb indicates 5-acetamido-3,5,7,9-tetradeoxy-7-[(R)-3-hydroxybutanoylamino]-l-glycero-l-manno-non-2-ulosonic acid.
27416015	4	5	dep	structure	415:423	arg1	-β-d-Galp-					519:528	→4)-β-Psep5Ac7Hb-(2 → 6)-β-d-Galf-(1 → 3)-β-d-Galp-	478:528	the following structure of the trisaccharide repeating unit was established: →4)-β-Psep5Ac7Hb-(2 → 6)-β-d-Galf-(1 → 3)-β-d-Galp-(1→ where Pse5Ac7Hb indicates 5-acetamido-3,5,7,9-tetradeoxy-7-[(R)-3-hydroxybutanoylamino]-l-glycero-l-manno-non-2-ulosonic acid	401:657	The polysaccharide was found to contain a derivative of pseudaminic acid (Pse) and the following structure of the trisaccharide repeating unit was established: →4)-β-Psep5Ac7Hb-(2 → 6)-β-d-Galf-(1 → 3)-β-d-Galp-(1→ where Pse5Ac7Hb indicates 5-acetamido-3,5,7,9-tetradeoxy-7-[(R)-3-hydroxybutanoylamino]-l-glycero-l-manno-non-2-ulosonic acid.
27416015	4	6	theme	repeating	446:454	arg1	unit					456:459	the trisaccharide repeating unit	428:459	the trisaccharide repeating unit	428:459	The polysaccharide was found to contain a derivative of pseudaminic acid (Pse) and the following structure of the trisaccharide repeating unit was established: →4)-β-Psep5Ac7Hb-(2 → 6)-β-d-Galf-(1 → 3)-β-d-Galp-(1→ where Pse5Ac7Hb indicates 5-acetamido-3,5,7,9-tetradeoxy-7-[(R)-3-hydroxybutanoylamino]-l-glycero-l-manno-non-2-ulosonic acid.
27416015	1	7	theme	chlororaphis	79:90	arg1	B-106					96:100	chlororaphis UCM B-106	79:100	chlororaphis UCM B-106.	79:101	chlororaphis UCM B-106.
27416015	1	8	theme	UCM	92:94	arg1	B-106					96:100	chlororaphis UCM B-106	79:100	chlororaphis UCM B-106.	79:101	chlororaphis UCM B-106.
27416015	4	9	theme	acid	654:657	arg1	derivative					360:369	a derivative	358:369	a derivative of pseudaminic acid (Pse) and the following structure of the trisaccharide repeating unit was established: →4)-β-Psep5Ac7Hb-(2 → 6)-β-d-Galf-(1 → 3)-β-d-Galp-(1→ where Pse5Ac7Hb indicates 5-acetamido-3,5,7,9-tetradeoxy-7-[(R)-3-hydroxybutanoylamino]-l-glycero-l-manno-non-2-ulosonic acid	358:657	The polysaccharide was found to contain a derivative of pseudaminic acid (Pse) and the following structure of the trisaccharide repeating unit was established: →4)-β-Psep5Ac7Hb-(2 → 6)-β-d-Galf-(1 → 3)-β-d-Galp-(1→ where Pse5Ac7Hb indicates 5-acetamido-3,5,7,9-tetradeoxy-7-[(R)-3-hydroxybutanoylamino]-l-glycero-l-manno-non-2-ulosonic acid.
27416015	2	10	theme	Pseudomonas	173:183	arg1	subsp					198:202	Pseudomonas chlororaphis subsp	173:202	Pseudomonas chlororaphis subsp	173:202	O-specific polysaccharide was obtained from the lipopolysaccharide of Pseudomonas chlororaphis subsp.
27416015	4	11	theme	1 → 3	513:517	arg1	-β-d-Galp-					519:528	→4)-β-Psep5Ac7Hb-(2 → 6)-β-d-Galf-(1 → 3)-β-d-Galp-	478:528	the following structure of the trisaccharide repeating unit was established: →4)-β-Psep5Ac7Hb-(2 → 6)-β-d-Galf-(1 → 3)-β-d-Galp-(1→ where Pse5Ac7Hb indicates 5-acetamido-3,5,7,9-tetradeoxy-7-[(R)-3-hydroxybutanoylamino]-l-glycero-l-manno-non-2-ulosonic acid	401:657	The polysaccharide was found to contain a derivative of pseudaminic acid (Pse) and the following structure of the trisaccharide repeating unit was established: →4)-β-Psep5Ac7Hb-(2 → 6)-β-d-Galf-(1 → 3)-β-d-Galp-(1→ where Pse5Ac7Hb indicates 5-acetamido-3,5,7,9-tetradeoxy-7-[(R)-3-hydroxybutanoylamino]-l-glycero-l-manno-non-2-ulosonic acid.
27416015	0	12	theme	O-specific	17:26	arg1	polysaccharides					28:42	the O-specific polysaccharides	13:42	the O-specific polysaccharides of Pseudomonas chlororaphis subsp	13:76	Structure of the O-specific polysaccharides of Pseudomonas chlororaphis subsp.
27416015	4	13	contain	contain	350:356	arg1	polysaccharide					322:335	The polysaccharide	318:335	The polysaccharide	318:335	The polysaccharide was found to contain a derivative of pseudaminic acid (Pse) and the following structure of the trisaccharide repeating unit was established: →4)-β-Psep5Ac7Hb-(2 → 6)-β-d-Galf-(1 → 3)-β-d-Galp-(1→ where Pse5Ac7Hb indicates 5-acetamido-3,5,7,9-tetradeoxy-7-[(R)-3-hydroxybutanoylamino]-l-glycero-l-manno-non-2-ulosonic acid.
27416015	4	13	contain	contain	350:356	arg2	derivative					360:369	a derivative	358:369	a derivative of pseudaminic acid (Pse) and the following structure of the trisaccharide repeating unit was established: →4)-β-Psep5Ac7Hb-(2 → 6)-β-d-Galf-(1 → 3)-β-d-Galp-(1→ where Pse5Ac7Hb indicates 5-acetamido-3,5,7,9-tetradeoxy-7-[(R)-3-hydroxybutanoylamino]-l-glycero-l-manno-non-2-ulosonic acid	358:657	The polysaccharide was found to contain a derivative of pseudaminic acid (Pse) and the following structure of the trisaccharide repeating unit was established: →4)-β-Psep5Ac7Hb-(2 → 6)-β-d-Galf-(1 → 3)-β-d-Galp-(1→ where Pse5Ac7Hb indicates 5-acetamido-3,5,7,9-tetradeoxy-7-[(R)-3-hydroxybutanoylamino]-l-glycero-l-manno-non-2-ulosonic acid.
27416015	4	14	theme	acid	386:389	arg1	derivative					360:369	a derivative	358:369	a derivative of pseudaminic acid (Pse) and the following structure of the trisaccharide repeating unit was established: →4)-β-Psep5Ac7Hb-(2 → 6)-β-d-Galf-(1 → 3)-β-d-Galp-(1→ where Pse5Ac7Hb indicates 5-acetamido-3,5,7,9-tetradeoxy-7-[(R)-3-hydroxybutanoylamino]-l-glycero-l-manno-non-2-ulosonic acid	358:657	The polysaccharide was found to contain a derivative of pseudaminic acid (Pse) and the following structure of the trisaccharide repeating unit was established: →4)-β-Psep5Ac7Hb-(2 → 6)-β-d-Galf-(1 → 3)-β-d-Galp-(1→ where Pse5Ac7Hb indicates 5-acetamido-3,5,7,9-tetradeoxy-7-[(R)-3-hydroxybutanoylamino]-l-glycero-l-manno-non-2-ulosonic acid.
27416015	2	15	dep	Pseudomonas	173:183	arg1	chlororaphis					185:196	chlororaphis	185:196	chlororaphis	185:196	O-specific polysaccharide was obtained from the lipopolysaccharide of Pseudomonas chlororaphis subsp.
27416015	4	16	theme	structure	415:423	arg1	acid					654:657	the following structure of the trisaccharide repeating unit was established: →4)-β-Psep5Ac7Hb-(2 → 6)-β-d-Galf-(1 → 3)-β-d-Galp-(1→ where Pse5Ac7Hb indicates 5-acetamido-3,5,7,9-tetradeoxy-7-[(R)-3-hydroxybutanoylamino]-l-glycero-l-manno-non-2-ulosonic acid	401:657	the following structure of the trisaccharide repeating unit was established: →4)-β-Psep5Ac7Hb-(2 → 6)-β-d-Galf-(1 → 3)-β-d-Galp-(1→ where Pse5Ac7Hb indicates 5-acetamido-3,5,7,9-tetradeoxy-7-[(R)-3-hydroxybutanoylamino]-l-glycero-l-manno-non-2-ulosonic acid	401:657	The polysaccharide was found to contain a derivative of pseudaminic acid (Pse) and the following structure of the trisaccharide repeating unit was established: →4)-β-Psep5Ac7Hb-(2 → 6)-β-d-Galf-(1 → 3)-β-d-Galp-(1→ where Pse5Ac7Hb indicates 5-acetamido-3,5,7,9-tetradeoxy-7-[(R)-3-hydroxybutanoylamino]-l-glycero-l-manno-non-2-ulosonic acid.
27416015	4	17	theme	-l-glycero-l-manno-non-2-ulosonic	620:652	arg1	acid					654:657	the following structure of the trisaccharide repeating unit was established: →4)-β-Psep5Ac7Hb-(2 → 6)-β-d-Galf-(1 → 3)-β-d-Galp-(1→ where Pse5Ac7Hb indicates 5-acetamido-3,5,7,9-tetradeoxy-7-[(R)-3-hydroxybutanoylamino]-l-glycero-l-manno-non-2-ulosonic acid	401:657	the following structure of the trisaccharide repeating unit was established: →4)-β-Psep5Ac7Hb-(2 → 6)-β-d-Galf-(1 → 3)-β-d-Galp-(1→ where Pse5Ac7Hb indicates 5-acetamido-3,5,7,9-tetradeoxy-7-[(R)-3-hydroxybutanoylamino]-l-glycero-l-manno-non-2-ulosonic acid	401:657	The polysaccharide was found to contain a derivative of pseudaminic acid (Pse) and the following structure of the trisaccharide repeating unit was established: →4)-β-Psep5Ac7Hb-(2 → 6)-β-d-Galf-(1 → 3)-β-d-Galp-(1→ where Pse5Ac7Hb indicates 5-acetamido-3,5,7,9-tetradeoxy-7-[(R)-3-hydroxybutanoylamino]-l-glycero-l-manno-non-2-ulosonic acid.
27416015	0	18	theme	polysaccharides	28:42	arg1	Structure					0:8	Structure	0:8	Structure of the O-specific polysaccharides of Pseudomonas chlororaphis subsp	0:76	Structure of the O-specific polysaccharides of Pseudomonas chlororaphis subsp.
27416015	4	19	theme	trisaccharide	432:444	arg1	unit					456:459	the trisaccharide repeating unit	428:459	the trisaccharide repeating unit	428:459	The polysaccharide was found to contain a derivative of pseudaminic acid (Pse) and the following structure of the trisaccharide repeating unit was established: →4)-β-Psep5Ac7Hb-(2 → 6)-β-d-Galf-(1 → 3)-β-d-Galp-(1→ where Pse5Ac7Hb indicates 5-acetamido-3,5,7,9-tetradeoxy-7-[(R)-3-hydroxybutanoylamino]-l-glycero-l-manno-non-2-ulosonic acid.
27416015	0	20	dep	Pseudomonas	47:57	arg1	chlororaphis					59:70	chlororaphis	59:70	chlororaphis	59:70	Structure of the O-specific polysaccharides of Pseudomonas chlororaphis subsp.
27416015	3	21	dep	2D	282:283	arg1	H					288:288	H	288:288	H	288:288	chlororaphis UCM B-106 and studied by composition analysis along with 1D and 2D (1)H and (13)C NMR spectroscopy.
27416015	3	21	dep	2D	282:283	arg1	1					286:286	1	286:286	1	286:286	chlororaphis UCM B-106 and studied by composition analysis along with 1D and 2D (1)H and (13)C NMR spectroscopy.
27416015	3	21	dep	2D	282:283	arg1	spectroscopy					304:315	(13)C NMR spectroscopy	294:315	(13)C NMR spectroscopy	294:315	chlororaphis UCM B-106 and studied by composition analysis along with 1D and 2D (1)H and (13)C NMR spectroscopy.
27416015	2	22	theme	O-specific	103:112	arg1	polysaccharide					114:127	O-specific polysaccharide	103:127	O-specific polysaccharide	103:127	O-specific polysaccharide was obtained from the lipopolysaccharide of Pseudomonas chlororaphis subsp.
27416015	2	23	theme	subsp	198:202	arg1	lipopolysaccharide					151:168	the lipopolysaccharide	147:168	the lipopolysaccharide of Pseudomonas chlororaphis subsp	147:202	O-specific polysaccharide was obtained from the lipopolysaccharide of Pseudomonas chlororaphis subsp.
27416015	0	24	theme	Pseudomonas	47:57	arg1	subsp					72:76	Pseudomonas chlororaphis subsp	47:76	Pseudomonas chlororaphis subsp	47:76	Structure of the O-specific polysaccharides of Pseudomonas chlororaphis subsp.
27416015	4	25	theme	-β-d-Galf-	502:511	arg1	-β-d-Galp-					519:528	→4)-β-Psep5Ac7Hb-(2 → 6)-β-d-Galf-(1 → 3)-β-d-Galp-	478:528	the following structure of the trisaccharide repeating unit was established: →4)-β-Psep5Ac7Hb-(2 → 6)-β-d-Galf-(1 → 3)-β-d-Galp-(1→ where Pse5Ac7Hb indicates 5-acetamido-3,5,7,9-tetradeoxy-7-[(R)-3-hydroxybutanoylamino]-l-glycero-l-manno-non-2-ulosonic acid	401:657	The polysaccharide was found to contain a derivative of pseudaminic acid (Pse) and the following structure of the trisaccharide repeating unit was established: →4)-β-Psep5Ac7Hb-(2 → 6)-β-d-Galf-(1 → 3)-β-d-Galp-(1→ where Pse5Ac7Hb indicates 5-acetamido-3,5,7,9-tetradeoxy-7-[(R)-3-hydroxybutanoylamino]-l-glycero-l-manno-non-2-ulosonic acid.
27416015	3	26	theme	C	298:298	arg1	spectroscopy					304:315	(13)C NMR spectroscopy	294:315	(13)C NMR spectroscopy	294:315	chlororaphis UCM B-106 and studied by composition analysis along with 1D and 2D (1)H and (13)C NMR spectroscopy.
27416015	4	27	theme	pseudaminic	374:384	arg1	acid					386:389	pseudaminic acid	374:389	pseudaminic acid (Pse)	374:395	The polysaccharide was found to contain a derivative of pseudaminic acid (Pse) and the following structure of the trisaccharide repeating unit was established: →4)-β-Psep5Ac7Hb-(2 → 6)-β-d-Galf-(1 → 3)-β-d-Galp-(1→ where Pse5Ac7Hb indicates 5-acetamido-3,5,7,9-tetradeoxy-7-[(R)-3-hydroxybutanoylamino]-l-glycero-l-manno-non-2-ulosonic acid.
27416015	4	27	theme	pseudaminic	374:384	arg1	Pse					392:394	Pse	392:394	Pse	392:394	The polysaccharide was found to contain a derivative of pseudaminic acid (Pse) and the following structure of the trisaccharide repeating unit was established: →4)-β-Psep5Ac7Hb-(2 → 6)-β-d-Galf-(1 → 3)-β-d-Galp-(1→ where Pse5Ac7Hb indicates 5-acetamido-3,5,7,9-tetradeoxy-7-[(R)-3-hydroxybutanoylamino]-l-glycero-l-manno-non-2-ulosonic acid.
27416015	4	28	theme	following	405:413	arg1	structure					415:423	the following structure	401:423	the following structure of the trisaccharide repeating unit was established: →4)-β-Psep5Ac7Hb-(2 → 6)-β-d-Galf-(1 → 3)-β-d-Galp-(1→ where Pse5Ac7Hb indicates 5-acetamido-3,5,7,9-tetradeoxy-7-[(R)-3-hydroxybutanoylamino]-l-glycero-l-manno-non-2-ulosonic acid	401:657	The polysaccharide was found to contain a derivative of pseudaminic acid (Pse) and the following structure of the trisaccharide repeating unit was established: →4)-β-Psep5Ac7Hb-(2 → 6)-β-d-Galf-(1 → 3)-β-d-Galp-(1→ where Pse5Ac7Hb indicates 5-acetamido-3,5,7,9-tetradeoxy-7-[(R)-3-hydroxybutanoylamino]-l-glycero-l-manno-non-2-ulosonic acid.
27416015	0	29	theme	subsp	72:76	arg1	polysaccharides					28:42	the O-specific polysaccharides	13:42	the O-specific polysaccharides of Pseudomonas chlororaphis subsp	13:76	Structure of the O-specific polysaccharides of Pseudomonas chlororaphis subsp.
26476169	0	0	theme	hemicellulose	80:92	arg1	Solubilization					62:75	Solubilization	62:75	Solubilization of hemicellulose and cellulose from holm oak wood	62:125	Modeling of biomass fractionation in a lab-scale biorefinery: Solubilization of hemicellulose and cellulose from holm oak wood using subcritical water.
26476169	1	1	theme	Lignocellulose	152:165	arg1	process					202:208	a key biorefinery process	184:208	a key biorefinery process that need to be understood	184:235	Lignocellulose fractionation is a key biorefinery process that need to be understood.
26476169	1	1	theme	Lignocellulose	152:165	arg1	fractionation					167:179	Lignocellulose fractionation	152:179	Lignocellulose fractionation	152:179	Lignocellulose fractionation is a key biorefinery process that need to be understood.
26476169	6	2	dep	deviation	949:957	arg1	e.g.					944:947	e.g.	944:947	e.g.	944:947	The kinetic model was validated against experimental data, reproducing the total organic carbon profile (e.g. deviation of 33%) and the physicochemical phenomena observed in the process.
26476169	6	3	theme	kinetic	843:849	arg1	model					851:855	The kinetic model	839:855	The kinetic model	839:855	The kinetic model was validated against experimental data, reproducing the total organic carbon profile (e.g. deviation of 33%) and the physicochemical phenomena observed in the process.
26476169	0	4	theme	cellulose	98:106	arg1	Solubilization					62:75	Solubilization	62:75	Solubilization of hemicellulose and cellulose from holm oak wood	62:125	Modeling of biomass fractionation in a lab-scale biorefinery: Solubilization of hemicellulose and cellulose from holm oak wood using subcritical water.
26476169	0	5	from	Modeling	0:7	arg1	biorefinery					49:59	a lab-scale biorefinery	37:59	a lab-scale biorefinery	37:59	Modeling of biomass fractionation in a lab-scale biorefinery: Solubilization of hemicellulose and cellulose from holm oak wood using subcritical water.
26476169	6	6	theme	carbon	928:933	arg1	profile					935:941	the total organic carbon profile	910:941	the total organic carbon profile (e.g. deviation of 33%)	910:965	The kinetic model was validated against experimental data, reproducing the total organic carbon profile (e.g. deviation of 33%) and the physicochemical phenomena observed in the process.
26476169	6	7	located	observed	1001:1008	arg2	phenomena					991:999	the physicochemical phenomena	971:999	the physicochemical phenomena observed in the process	971:1023	The kinetic model was validated against experimental data, reproducing the total organic carbon profile (e.g. deviation of 33%) and the physicochemical phenomena observed in the process.
26476169	6	7	located	observed	1001:1008	arg1	process					1017:1023	the process	1013:1023	the process	1013:1023	The kinetic model was validated against experimental data, reproducing the total organic carbon profile (e.g. deviation of 33%) and the physicochemical phenomena observed in the process.
26476169	4	8	theme	different	676:684	arg1	flows					686:690	different flows	676:690	different flows	676:690	The experiments involved two sets: at constant flow (6mL/min) and two different ranges of temperature (140-180 and 240-280°C) and at a constant temperature range (180-260°C) and different flows: 11.0, 15.0 and 27.9mL/min.
26476169	6	9	theme	organic	920:926	arg1	profile					935:941	the total organic carbon profile	910:941	the total organic carbon profile (e.g. deviation of 33%)	910:965	The kinetic model was validated against experimental data, reproducing the total organic carbon profile (e.g. deviation of 33%) and the physicochemical phenomena observed in the process.
26476169	0	10	theme	holm	113:116	arg1	wood					122:125	holm oak wood	113:125	holm oak wood	113:125	Modeling of biomass fractionation in a lab-scale biorefinery: Solubilization of hemicellulose and cellulose from holm oak wood using subcritical water.
26476169	5	11	theme	flow	773:776	arg1	effect					778:783	flow effect	773:783	flow effect	773:783	From the results, temperature has main influence and flow effect was observed only if soluble compounds were produced.
26476169	2	12	theme	comprehensive	254:266	arg1	study					268:272	a comprehensive study	252:272	a comprehensive study on hydrothermal-fractionation of holm oak in a semi-continuous system	252:342	In this work, a comprehensive study on hydrothermal-fractionation of holm oak in a semi-continuous system was conducted.
26476169	7	13	theme	molecular	1081:1089	arg1	weight					1091:1096	molecular weight	1081:1096	molecular weight of each biopolymer	1081:1115	In the model, it was also considered the variations of molecular weight of each biopolymer, successfully reproducing the biomass cleaving.
26476169	6	14	theme	total	914:918	arg1	profile					935:941	the total organic carbon profile	910:941	the total organic carbon profile (e.g. deviation of 33%)	910:965	The kinetic model was validated against experimental data, reproducing the total organic carbon profile (e.g. deviation of 33%) and the physicochemical phenomena observed in the process.
26476169	1	15	theme	key	186:188	arg1	process					202:208	a key biorefinery process	184:208	a key biorefinery process that need to be understood	184:235	Lignocellulose fractionation is a key biorefinery process that need to be understood.
26476169	1	15	theme	key	186:188	arg1	fractionation					167:179	Lignocellulose fractionation	152:179	Lignocellulose fractionation	152:179	Lignocellulose fractionation is a key biorefinery process that need to be understood.
26476169	7	16	theme	weight	1091:1096	arg1	variations					1067:1076	the variations	1063:1076	the variations	1063:1076	In the model, it was also considered the variations of molecular weight of each biopolymer, successfully reproducing the biomass cleaving.
26476169	4	17	theme	constant	536:543	arg1	6mL/min					551:557	6mL/min	551:557	6mL/min	551:557	The experiments involved two sets: at constant flow (6mL/min) and two different ranges of temperature (140-180 and 240-280°C) and at a constant temperature range (180-260°C) and different flows: 11.0, 15.0 and 27.9mL/min.
26476169	4	17	theme	constant	536:543	arg1	flow					545:548	constant flow	536:548	constant flow (6mL/min)	536:558	The experiments involved two sets: at constant flow (6mL/min) and two different ranges of temperature (140-180 and 240-280°C) and at a constant temperature range (180-260°C) and different flows: 11.0, 15.0 and 27.9mL/min.
26476169	2	18	theme	oak	312:314	arg1	hydrothermal-fractionation					277:302	hydrothermal-fractionation	277:302	hydrothermal-fractionation of holm oak	277:314	In this work, a comprehensive study on hydrothermal-fractionation of holm oak in a semi-continuous system was conducted.
26476169	1	19	theme	biorefinery	190:200	arg1	process					202:208	a key biorefinery process	184:208	a key biorefinery process that need to be understood	184:235	Lignocellulose fractionation is a key biorefinery process that need to be understood.
26476169	1	19	theme	biorefinery	190:200	arg1	fractionation					167:179	Lignocellulose fractionation	152:179	Lignocellulose fractionation	152:179	Lignocellulose fractionation is a key biorefinery process that need to be understood.
26476169	0	20	theme	fractionation	20:32	arg1	Modeling					0:7	Modeling	0:7	Modeling of biomass fractionation in a lab-scale biorefinery	0:59	Modeling of biomass fractionation in a lab-scale biorefinery: Solubilization of hemicellulose and cellulose from holm oak wood using subcritical water.
26476169	2	21	theme	holm	307:310	arg1	oak					312:314	holm oak	307:314	holm oak	307:314	In this work, a comprehensive study on hydrothermal-fractionation of holm oak in a semi-continuous system was conducted.
26476169	0	22	theme	biomass	12:18	arg1	fractionation					20:32	biomass fractionation	12:32	biomass fractionation	12:32	Modeling of biomass fractionation in a lab-scale biorefinery: Solubilization of hemicellulose and cellulose from holm oak wood using subcritical water.
26476169	0	23	theme	oak	118:120	arg1	wood					122:125	holm oak wood	113:125	holm oak wood	113:125	Modeling of biomass fractionation in a lab-scale biorefinery: Solubilization of hemicellulose and cellulose from holm oak wood using subcritical water.
26476169	5	24	theme	main	754:757	arg1	influence					759:767	main influence	754:767	main influence	754:767	From the results, temperature has main influence and flow effect was observed only if soluble compounds were produced.
26476169	3	25	theme	water	456:460	arg1	flow					462:465	water flow	456:465	water flow	456:465	The aim was to develop a physicochemical model in order to reproduce the role of temperature and water flow over the products composition.
26476169	7	26	theme	biopolymer	1106:1115	arg1	weight					1091:1096	molecular weight	1081:1096	molecular weight of each biopolymer	1081:1115	In the model, it was also considered the variations of molecular weight of each biopolymer, successfully reproducing the biomass cleaving.
26476169	0	27	theme	subcritical	133:143	arg1	water					145:149	subcritical water	133:149	subcritical water	133:149	Modeling of biomass fractionation in a lab-scale biorefinery: Solubilization of hemicellulose and cellulose from holm oak wood using subcritical water.
26476169	5	28	theme	soluble	806:812	arg1	compounds					814:822	soluble compounds	806:822	soluble compounds	806:822	From the results, temperature has main influence and flow effect was observed only if soluble compounds were produced.
26476169	5	29	contain	has	750:752	arg2	influence					759:767	main influence	754:767	main influence	754:767	From the results, temperature has main influence and flow effect was observed only if soluble compounds were produced.
26476169	5	29	contain	has	750:752	arg1	temperature					738:748	temperature	738:748	temperature	738:748	From the results, temperature has main influence and flow effect was observed only if soluble compounds were produced.
26476169	4	30	theme	temperature	588:598	arg1	ranges					578:583	two different ranges	564:583	two different ranges of temperature (140-180 and 240-280°C)	564:622	The experiments involved two sets: at constant flow (6mL/min) and two different ranges of temperature (140-180 and 240-280°C) and at a constant temperature range (180-260°C) and different flows: 11.0, 15.0 and 27.9mL/min.
26476169	3	31	theme	temperature	440:450	arg1	role					432:435	the role	428:435	the role of temperature and water flow	428:465	The aim was to develop a physicochemical model in order to reproduce the role of temperature and water flow over the products composition.
26476169	3	32	theme	physicochemical	384:398	arg1	model					400:404	a physicochemical model	382:404	a physicochemical model in order to reproduce the role of temperature and water flow over the products composition	382:495	The aim was to develop a physicochemical model in order to reproduce the role of temperature and water flow over the products composition.
26476169	6	33	theme	experimental	879:890	arg1	data					892:895	experimental data	879:895	experimental data	879:895	The kinetic model was validated against experimental data, reproducing the total organic carbon profile (e.g. deviation of 33%) and the physicochemical phenomena observed in the process.
26476169	0	34	theme	lab-scale	39:47	arg1	biorefinery					49:59	a lab-scale biorefinery	37:59	a lab-scale biorefinery	37:59	Modeling of biomass fractionation in a lab-scale biorefinery: Solubilization of hemicellulose and cellulose from holm oak wood using subcritical water.
26476169	3	35	theme	products	476:483	arg1	composition					485:495	the products composition	472:495	the products composition	472:495	The aim was to develop a physicochemical model in order to reproduce the role of temperature and water flow over the products composition.
26476169	2	36	from	study	268:272	arg1	system					337:342	a semi-continuous system	319:342	a semi-continuous system	319:342	In this work, a comprehensive study on hydrothermal-fractionation of holm oak in a semi-continuous system was conducted.
26476169	2	36	from	study	268:272	arg1	hydrothermal-fractionation					277:302	hydrothermal-fractionation	277:302	hydrothermal-fractionation of holm oak	277:314	In this work, a comprehensive study on hydrothermal-fractionation of holm oak in a semi-continuous system was conducted.
26476169	2	37	theme	semi-continuous	321:335	arg1	system					337:342	a semi-continuous system	319:342	a semi-continuous system	319:342	In this work, a comprehensive study on hydrothermal-fractionation of holm oak in a semi-continuous system was conducted.
26476169	4	38	dep	ranges	578:583	arg1	240-280°C					613:621	240-280°C	613:621	240-280°C	613:621	The experiments involved two sets: at constant flow (6mL/min) and two different ranges of temperature (140-180 and 240-280°C) and at a constant temperature range (180-260°C) and different flows: 11.0, 15.0 and 27.9mL/min.
26476169	4	38	dep	ranges	578:583	arg1	140-180					601:607	140-180	601:607	140-180	601:607	The experiments involved two sets: at constant flow (6mL/min) and two different ranges of temperature (140-180 and 240-280°C) and at a constant temperature range (180-260°C) and different flows: 11.0, 15.0 and 27.9mL/min.
26476169	6	39	theme	physicochemical	975:989	arg1	phenomena					991:999	the physicochemical phenomena	971:999	the physicochemical phenomena observed in the process	971:1023	The kinetic model was validated against experimental data, reproducing the total organic carbon profile (e.g. deviation of 33%) and the physicochemical phenomena observed in the process.
26476169	4	40	theme	constant	633:640	arg1	range					654:658	a constant temperature range	631:658	a constant temperature range (180-260°C)	631:670	The experiments involved two sets: at constant flow (6mL/min) and two different ranges of temperature (140-180 and 240-280°C) and at a constant temperature range (180-260°C) and different flows: 11.0, 15.0 and 27.9mL/min.
26476169	4	40	theme	constant	633:640	arg1	180-260°C					661:669	180-260°C	661:669	180-260°C	661:669	The experiments involved two sets: at constant flow (6mL/min) and two different ranges of temperature (140-180 and 240-280°C) and at a constant temperature range (180-260°C) and different flows: 11.0, 15.0 and 27.9mL/min.
26476169	3	41	theme	flow	462:465	arg1	role					432:435	the role	428:435	the role of temperature and water flow	428:465	The aim was to develop a physicochemical model in order to reproduce the role of temperature and water flow over the products composition.
26476169	6	42	theme	%	964:964	arg1	deviation					949:957	deviation	949:957	deviation of 33%	949:964	The kinetic model was validated against experimental data, reproducing the total organic carbon profile (e.g. deviation of 33%) and the physicochemical phenomena observed in the process.
26476169	4	43	theme	temperature	642:652	arg1	range					654:658	a constant temperature range	631:658	a constant temperature range (180-260°C)	631:670	The experiments involved two sets: at constant flow (6mL/min) and two different ranges of temperature (140-180 and 240-280°C) and at a constant temperature range (180-260°C) and different flows: 11.0, 15.0 and 27.9mL/min.
26476169	4	43	theme	temperature	642:652	arg1	180-260°C					661:669	180-260°C	661:669	180-260°C	661:669	The experiments involved two sets: at constant flow (6mL/min) and two different ranges of temperature (140-180 and 240-280°C) and at a constant temperature range (180-260°C) and different flows: 11.0, 15.0 and 27.9mL/min.
26476169	0	44	from	wood	122:125	arg1	Solubilization					62:75	Solubilization	62:75	Solubilization of hemicellulose and cellulose from holm oak wood	62:125	Modeling of biomass fractionation in a lab-scale biorefinery: Solubilization of hemicellulose and cellulose from holm oak wood using subcritical water.
26476169	7	45	theme	biomass	1147:1153	arg1	cleaving					1155:1162	the biomass cleaving	1143:1162	the biomass cleaving	1143:1162	In the model, it was also considered the variations of molecular weight of each biopolymer, successfully reproducing the biomass cleaving.
26476169	4	46	theme	different	568:576	arg1	ranges					578:583	two different ranges	564:583	two different ranges of temperature (140-180 and 240-280°C)	564:622	The experiments involved two sets: at constant flow (6mL/min) and two different ranges of temperature (140-180 and 240-280°C) and at a constant temperature range (180-260°C) and different flows: 11.0, 15.0 and 27.9mL/min.
26476169	0	47	dep	using	127:131	arg1	Modeling					0:7	Modeling	0:7	Modeling of biomass fractionation in a lab-scale biorefinery	0:59	Modeling of biomass fractionation in a lab-scale biorefinery: Solubilization of hemicellulose and cellulose from holm oak wood using subcritical water.
28698076	5	0	theme	CPN	717:719	arg1	composites					721:730	The CPN composites	713:730	The CPN composites	713:730	The CPN composites were characterized for physical properties like Thickness, Barrier properties and mechanical testing.
28698076	8	1	theme	composite	1074:1082	arg1	Cytotoxicity					1054:1065	Cytotoxicity	1054:1065	Cytotoxicity of the composite	1054:1082	Cytotoxicity of the composite has been analyzed in normal mouse embryonic fibroblast cells.
28698076	10	2	theme	wound	1358:1362	arg1	healing					1364:1370	a wound healing	1356:1370	a wound healing material	1356:1379	The biological study suggests that CPN3% composite may be a potential candidate as a wound healing material for biomedical application.
28698076	3	3	theme	nanocellulose	545:557	arg1	size					533:536	the average particle size	512:536	the average particle size of the nanocellulose is 6.1nm	512:566	TEM images revealed the average particle size of the nanocellulose is 6.1nm.
28698076	9	4	theme	CPN3	1173:1176	arg1	composite					1179:1187	the CPN3% composite	1169:1187	the CPN3% composite	1169:1187	The results have shown the CPN3% composite shows a high level of antibacterial property when compared to the other composites.
28698076	2	5	theme	Thermo	340:345	arg1	TGA					369:371	TGA	369:371	TGA	369:371	The prepared bionanocomposites were characterized by Transmission electron microscopy (TEM), Thermo gravimetric analysis (TGA), X-ray diffraction (XRD) and Attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) spectra.
28698076	2	5	theme	Thermo	340:345	arg1	analysis					359:366	Thermo gravimetric analysis	340:366	Thermo gravimetric analysis (TGA)	340:372	The prepared bionanocomposites were characterized by Transmission electron microscopy (TEM), Thermo gravimetric analysis (TGA), X-ray diffraction (XRD) and Attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) spectra.
28698076	10	6	theme	%	1312:1312	arg1	candidate					1343:1351	a potential candidate	1331:1351	a potential candidate as a wound healing material for biomedical application	1331:1406	The biological study suggests that CPN3% composite may be a potential candidate as a wound healing material for biomedical application.
28698076	10	6	theme	%	1312:1312	arg1	composite					1314:1322	CPN3% composite	1308:1322	CPN3% composite	1308:1322	The biological study suggests that CPN3% composite may be a potential candidate as a wound healing material for biomedical application.
28698076	5	7	theme	mechanical	814:823	arg1	testing					825:831	mechanical testing	814:831	mechanical testing	814:831	The CPN composites were characterized for physical properties like Thickness, Barrier properties and mechanical testing.
28698076	8	8	theme	normal	1105:1110	arg1	cells					1139:1143	normal mouse embryonic fibroblast cells	1105:1143	normal mouse embryonic fibroblast cells	1105:1143	Cytotoxicity of the composite has been analyzed in normal mouse embryonic fibroblast cells.
28698076	4	9	theme	composites	640:649	arg1	stability					623:631	the thermal stability	611:631	the thermal stability of the composites	611:649	Thermogravimetric analysis indicated that the thermal stability of the composites was decreased with increasing concentration of nanocellulose.
28698076	2	10	theme	X-ray	375:379	arg1	XRD					394:396	XRD	394:396	XRD	394:396	The prepared bionanocomposites were characterized by Transmission electron microscopy (TEM), Thermo gravimetric analysis (TGA), X-ray diffraction (XRD) and Attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) spectra.
28698076	2	10	theme	X-ray	375:379	arg1	diffraction					381:391	X-ray diffraction	375:391	X-ray diffraction (XRD)	375:397	The prepared bionanocomposites were characterized by Transmission electron microscopy (TEM), Thermo gravimetric analysis (TGA), X-ray diffraction (XRD) and Attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) spectra.
28698076	6	11	theme	CPN	897:899	arg1	composite					901:909	CPN composite	897:909	CPN composite	897:909	Water vapor and oxygen permeability evaluations indicated that CPN composite could maintain a moist environment over wound bed.
28698076	2	12	dep	microscopy	322:331	arg1	spectra					483:489	infrared spectroscopy (ATR-FTIR) spectra	450:489	infrared spectroscopy (ATR-FTIR) spectra	450:489	The prepared bionanocomposites were characterized by Transmission electron microscopy (TEM), Thermo gravimetric analysis (TGA), X-ray diffraction (XRD) and Attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) spectra.
28698076	10	13	theme	CPN3	1308:1311	arg1	candidate					1343:1351	a potential candidate	1331:1351	a potential candidate as a wound healing material for biomedical application	1331:1406	The biological study suggests that CPN3% composite may be a potential candidate as a wound healing material for biomedical application.
28698076	10	13	theme	CPN3	1308:1311	arg1	composite					1314:1322	CPN3% composite	1308:1322	CPN3% composite	1308:1322	The biological study suggests that CPN3% composite may be a potential candidate as a wound healing material for biomedical application.
28698076	8	14	theme	embryonic	1118:1126	arg1	cells					1139:1143	normal mouse embryonic fibroblast cells	1105:1143	normal mouse embryonic fibroblast cells	1105:1143	Cytotoxicity of the composite has been analyzed in normal mouse embryonic fibroblast cells.
28698076	2	15	theme	total	414:418	arg1	reflectance-Fourier					420:438	Attenuated total reflectance-Fourier transform	403:448	Attenuated total reflectance-Fourier transform	403:448	The prepared bionanocomposites were characterized by Transmission electron microscopy (TEM), Thermo gravimetric analysis (TGA), X-ray diffraction (XRD) and Attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) spectra.
28698076	8	16	theme	mouse	1112:1116	arg1	cells					1139:1143	normal mouse embryonic fibroblast cells	1105:1143	normal mouse embryonic fibroblast cells	1105:1143	Cytotoxicity of the composite has been analyzed in normal mouse embryonic fibroblast cells.
28698076	1	17	theme	Biocompatible	113:125	arg1	/Nanocellulose					160:173	Biocompatible Chitosan/Poly (vinyl pyrrolidone)/Nanocellulose	113:173	Biocompatible Chitosan/Poly (vinyl pyrrolidone)/Nanocellulose (CPN) composites	113:190	Biocompatible Chitosan/Poly (vinyl pyrrolidone)/Nanocellulose (CPN) composites were successfully prepared by solution casting method.
28698076	1	17	theme	Biocompatible	113:125	arg1	CPN					176:178	CPN	176:178	CPN	176:178	Biocompatible Chitosan/Poly (vinyl pyrrolidone)/Nanocellulose (CPN) composites were successfully prepared by solution casting method.
28698076	9	18	theme	high	1197:1200	arg1	level					1202:1206	a high level	1195:1206	a high level of antibacterial property	1195:1232	The results have shown the CPN3% composite shows a high level of antibacterial property when compared to the other composites.
28698076	2	19	theme	Attenuated	403:412	arg1	reflectance-Fourier					420:438	Attenuated total reflectance-Fourier transform	403:448	Attenuated total reflectance-Fourier transform	403:448	The prepared bionanocomposites were characterized by Transmission electron microscopy (TEM), Thermo gravimetric analysis (TGA), X-ray diffraction (XRD) and Attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) spectra.
28698076	7	20	theme	antibacterial	1030:1042	arg1	activity					1044:1051	antibacterial activity	1030:1051	antibacterial activity	1030:1051	The nanocomposite showed enhanced swelling, blood compatibility and antibacterial activity.
28698076	1	21	theme	Chitosan/Poly	127:139	arg1	/Nanocellulose					160:173	Biocompatible Chitosan/Poly (vinyl pyrrolidone)/Nanocellulose	113:173	Biocompatible Chitosan/Poly (vinyl pyrrolidone)/Nanocellulose (CPN) composites	113:190	Biocompatible Chitosan/Poly (vinyl pyrrolidone)/Nanocellulose (CPN) composites were successfully prepared by solution casting method.
28698076	1	21	theme	Chitosan/Poly	127:139	arg1	CPN					176:178	CPN	176:178	CPN	176:178	Biocompatible Chitosan/Poly (vinyl pyrrolidone)/Nanocellulose (CPN) composites were successfully prepared by solution casting method.
28698076	1	22	theme	solution	222:229	arg1	method					239:244	solution casting method	222:244	solution casting method	222:244	Biocompatible Chitosan/Poly (vinyl pyrrolidone)/Nanocellulose (CPN) composites were successfully prepared by solution casting method.
28698076	10	23	theme	potential	1333:1341	arg1	candidate					1343:1351	a potential candidate	1331:1351	a potential candidate as a wound healing material for biomedical application	1331:1406	The biological study suggests that CPN3% composite may be a potential candidate as a wound healing material for biomedical application.
28698076	10	23	theme	potential	1333:1341	arg1	composite					1314:1322	CPN3% composite	1308:1322	CPN3% composite	1308:1322	The biological study suggests that CPN3% composite may be a potential candidate as a wound healing material for biomedical application.
28698076	2	24	dep	reflectance-Fourier	420:438	arg1	transform					440:448	transform	440:448	transform	440:448	The prepared bionanocomposites were characterized by Transmission electron microscopy (TEM), Thermo gravimetric analysis (TGA), X-ray diffraction (XRD) and Attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) spectra.
28698076	6	25	theme	permeability	857:868	arg1	evaluations					870:880	oxygen permeability evaluations	850:880	oxygen permeability evaluations	850:880	Water vapor and oxygen permeability evaluations indicated that CPN composite could maintain a moist environment over wound bed.
28698076	2	26	theme	gravimetric	347:357	arg1	TGA					369:371	TGA	369:371	TGA	369:371	The prepared bionanocomposites were characterized by Transmission electron microscopy (TEM), Thermo gravimetric analysis (TGA), X-ray diffraction (XRD) and Attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) spectra.
28698076	2	26	theme	gravimetric	347:357	arg1	analysis					359:366	Thermo gravimetric analysis	340:366	Thermo gravimetric analysis (TGA)	340:372	The prepared bionanocomposites were characterized by Transmission electron microscopy (TEM), Thermo gravimetric analysis (TGA), X-ray diffraction (XRD) and Attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) spectra.
28698076	1	27	theme	casting	231:237	arg1	method					239:244	solution casting method	222:244	solution casting method	222:244	Biocompatible Chitosan/Poly (vinyl pyrrolidone)/Nanocellulose (CPN) composites were successfully prepared by solution casting method.
28698076	2	28	theme	ATR-FTIR	473:480	arg1	spectra					483:489	infrared spectroscopy (ATR-FTIR) spectra	450:489	infrared spectroscopy (ATR-FTIR) spectra	450:489	The prepared bionanocomposites were characterized by Transmission electron microscopy (TEM), Thermo gravimetric analysis (TGA), X-ray diffraction (XRD) and Attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) spectra.
28698076	1	29	theme	vinyl	142:146	arg1	/Nanocellulose					160:173	Biocompatible Chitosan/Poly (vinyl pyrrolidone)/Nanocellulose	113:173	Biocompatible Chitosan/Poly (vinyl pyrrolidone)/Nanocellulose (CPN) composites	113:190	Biocompatible Chitosan/Poly (vinyl pyrrolidone)/Nanocellulose (CPN) composites were successfully prepared by solution casting method.
28698076	1	29	theme	vinyl	142:146	arg1	CPN					176:178	CPN	176:178	CPN	176:178	Biocompatible Chitosan/Poly (vinyl pyrrolidone)/Nanocellulose (CPN) composites were successfully prepared by solution casting method.
28698076	9	30	theme	antibacterial	1211:1223	arg1	property					1225:1232	antibacterial property	1211:1232	antibacterial property	1211:1232	The results have shown the CPN3% composite shows a high level of antibacterial property when compared to the other composites.
28698076	2	31	theme	spectroscopy	459:470	arg1	spectra					483:489	infrared spectroscopy (ATR-FTIR) spectra	450:489	infrared spectroscopy (ATR-FTIR) spectra	450:489	The prepared bionanocomposites were characterized by Transmission electron microscopy (TEM), Thermo gravimetric analysis (TGA), X-ray diffraction (XRD) and Attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) spectra.
28698076	0	32	theme	composites	61:70	arg1	characterization					14:29	characterization	14:29	characterization	14:29	Synthesis and characterization of chitosan-PVP-nanocellulose composites for in-vitro wound dressing application.
28698076	0	32	theme	composites	61:70	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis and characterization of chitosan-PVP-nanocellulose composites for in-vitro wound dressing application.
28698076	4	33	theme	thermal	615:621	arg1	stability					623:631	the thermal stability	611:631	the thermal stability of the composites	611:649	Thermogravimetric analysis indicated that the thermal stability of the composites was decreased with increasing concentration of nanocellulose.
28698076	5	34	theme	Barrier	791:797	arg1	properties					799:808	Barrier properties	791:808	Barrier properties	791:808	The CPN composites were characterized for physical properties like Thickness, Barrier properties and mechanical testing.
28698076	3	35	theme	TEM	492:494	arg1	images					496:501	TEM images	492:501	TEM images	492:501	TEM images revealed the average particle size of the nanocellulose is 6.1nm.
28698076	9	36	theme	property	1225:1232	arg1	level					1202:1206	a high level	1195:1206	a high level of antibacterial property	1195:1232	The results have shown the CPN3% composite shows a high level of antibacterial property when compared to the other composites.
28698076	2	37	theme	infrared	450:457	arg1	spectra					483:489	infrared spectroscopy (ATR-FTIR) spectra	450:489	infrared spectroscopy (ATR-FTIR) spectra	450:489	The prepared bionanocomposites were characterized by Transmission electron microscopy (TEM), Thermo gravimetric analysis (TGA), X-ray diffraction (XRD) and Attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) spectra.
28698076	0	38	theme	chitosan-PVP-nanocellulose	34:59	arg1	composites					61:70	chitosan-PVP-nanocellulose composites	34:70	chitosan-PVP-nanocellulose composites	34:70	Synthesis and characterization of chitosan-PVP-nanocellulose composites for in-vitro wound dressing application.
28698076	4	39	theme	Thermogravimetric	569:585	arg1	analysis					587:594	Thermogravimetric analysis	569:594	Thermogravimetric analysis	569:594	Thermogravimetric analysis indicated that the thermal stability of the composites was decreased with increasing concentration of nanocellulose.
28698076	6	40	theme	oxygen	850:855	arg1	evaluations					870:880	oxygen permeability evaluations	850:880	oxygen permeability evaluations	850:880	Water vapor and oxygen permeability evaluations indicated that CPN composite could maintain a moist environment over wound bed.
28698076	0	41	theme	in-vitro	76:83	arg1	application					100:110	in-vitro wound dressing application	76:110	in-vitro wound dressing application	76:110	Synthesis and characterization of chitosan-PVP-nanocellulose composites for in-vitro wound dressing application.
28698076	6	42	theme	wound	951:955	arg1	bed					957:959	wound bed	951:959	wound bed	951:959	Water vapor and oxygen permeability evaluations indicated that CPN composite could maintain a moist environment over wound bed.
28698076	1	43	theme	pyrrolidone	148:158	arg1	/Nanocellulose					160:173	Biocompatible Chitosan/Poly (vinyl pyrrolidone)/Nanocellulose	113:173	Biocompatible Chitosan/Poly (vinyl pyrrolidone)/Nanocellulose (CPN) composites	113:190	Biocompatible Chitosan/Poly (vinyl pyrrolidone)/Nanocellulose (CPN) composites were successfully prepared by solution casting method.
28698076	1	43	theme	pyrrolidone	148:158	arg1	CPN					176:178	CPN	176:178	CPN	176:178	Biocompatible Chitosan/Poly (vinyl pyrrolidone)/Nanocellulose (CPN) composites were successfully prepared by solution casting method.
28698076	5	44	theme	physical	755:762	arg1	properties					764:773	physical properties	755:773	physical properties like Thickness, Barrier properties and mechanical testing	755:831	The CPN composites were characterized for physical properties like Thickness, Barrier properties and mechanical testing.
28698076	10	45	theme	biomedical	1385:1394	arg1	application					1396:1406	biomedical application	1385:1406	biomedical application	1385:1406	The biological study suggests that CPN3% composite may be a potential candidate as a wound healing material for biomedical application.
28698076	2	46	theme	prepared	251:258	arg1	bionanocomposites					260:276	The prepared bionanocomposites	247:276	The prepared bionanocomposites	247:276	The prepared bionanocomposites were characterized by Transmission electron microscopy (TEM), Thermo gravimetric analysis (TGA), X-ray diffraction (XRD) and Attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) spectra.
28698076	3	47	dep	nanocellulose	545:557	arg1	6.1nm					562:566	6.1nm	562:566	6.1nm	562:566	TEM images revealed the average particle size of the nanocellulose is 6.1nm.
28698076	8	48	theme	fibroblast	1128:1137	arg1	cells					1139:1143	normal mouse embryonic fibroblast cells	1105:1143	normal mouse embryonic fibroblast cells	1105:1143	Cytotoxicity of the composite has been analyzed in normal mouse embryonic fibroblast cells.
28698076	6	49	theme	Water	834:838	arg1	vapor					840:844	Water vapor	834:844	Water vapor	834:844	Water vapor and oxygen permeability evaluations indicated that CPN composite could maintain a moist environment over wound bed.
28698076	1	50	theme	/Nanocellulose	160:173	arg1	composites					181:190	Biocompatible Chitosan/Poly (vinyl pyrrolidone)/Nanocellulose (CPN) composites	113:190	Biocompatible Chitosan/Poly (vinyl pyrrolidone)/Nanocellulose (CPN) composites	113:190	Biocompatible Chitosan/Poly (vinyl pyrrolidone)/Nanocellulose (CPN) composites were successfully prepared by solution casting method.
28698076	10	51	theme	healing	1364:1370	arg1	material					1372:1379	a wound healing material	1356:1379	a wound healing material	1356:1379	The biological study suggests that CPN3% composite may be a potential candidate as a wound healing material for biomedical application.
28698076	6	52	theme	moist	928:932	arg1	environment					934:944	a moist environment	926:944	a moist environment	926:944	Water vapor and oxygen permeability evaluations indicated that CPN composite could maintain a moist environment over wound bed.
28698076	2	53	theme	electron	313:320	arg1	TEM					334:336	TEM	334:336	TEM	334:336	The prepared bionanocomposites were characterized by Transmission electron microscopy (TEM), Thermo gravimetric analysis (TGA), X-ray diffraction (XRD) and Attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) spectra.
28698076	2	53	theme	electron	313:320	arg1	microscopy					322:331	Transmission electron microscopy	300:331	Transmission electron microscopy (TEM)	300:337	The prepared bionanocomposites were characterized by Transmission electron microscopy (TEM), Thermo gravimetric analysis (TGA), X-ray diffraction (XRD) and Attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) spectra.
28698076	9	54	theme	other	1255:1259	arg1	composites					1261:1270	the other composites	1251:1270	the other composites	1251:1270	The results have shown the CPN3% composite shows a high level of antibacterial property when compared to the other composites.
28698076	3	55	theme	particle	524:531	arg1	size					533:536	the average particle size	512:536	the average particle size of the nanocellulose is 6.1nm	512:566	TEM images revealed the average particle size of the nanocellulose is 6.1nm.
28698076	3	56	theme	average	516:522	arg1	size					533:536	the average particle size	512:536	the average particle size of the nanocellulose is 6.1nm	512:566	TEM images revealed the average particle size of the nanocellulose is 6.1nm.
28698076	0	57	theme	dressing	91:98	arg1	application					100:110	in-vitro wound dressing application	76:110	in-vitro wound dressing application	76:110	Synthesis and characterization of chitosan-PVP-nanocellulose composites for in-vitro wound dressing application.
28698076	9	58	theme	%	1177:1177	arg1	composite					1179:1187	the CPN3% composite	1169:1187	the CPN3% composite	1169:1187	The results have shown the CPN3% composite shows a high level of antibacterial property when compared to the other composites.
28698076	2	59	theme	Transmission	300:311	arg1	TEM					334:336	TEM	334:336	TEM	334:336	The prepared bionanocomposites were characterized by Transmission electron microscopy (TEM), Thermo gravimetric analysis (TGA), X-ray diffraction (XRD) and Attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) spectra.
28698076	2	59	theme	Transmission	300:311	arg1	microscopy					322:331	Transmission electron microscopy	300:331	Transmission electron microscopy (TEM)	300:337	The prepared bionanocomposites were characterized by Transmission electron microscopy (TEM), Thermo gravimetric analysis (TGA), X-ray diffraction (XRD) and Attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) spectra.
28698076	4	60	theme	nanocellulose	698:710	arg1	concentration					681:693	concentration	681:693	concentration of nanocellulose	681:710	Thermogravimetric analysis indicated that the thermal stability of the composites was decreased with increasing concentration of nanocellulose.
28698076	7	61	theme	blood	1006:1010	arg1	compatibility					1012:1024	blood compatibility	1006:1024	blood compatibility	1006:1024	The nanocomposite showed enhanced swelling, blood compatibility and antibacterial activity.
28698076	0	62	theme	wound	85:89	arg1	application					100:110	in-vitro wound dressing application	76:110	in-vitro wound dressing application	76:110	Synthesis and characterization of chitosan-PVP-nanocellulose composites for in-vitro wound dressing application.
28698076	7	63	theme	enhanced	987:994	arg1	swelling					996:1003	enhanced swelling	987:1003	enhanced swelling	987:1003	The nanocomposite showed enhanced swelling, blood compatibility and antibacterial activity.
28698076	10	64	theme	biological	1277:1286	arg1	study					1288:1292	The biological study	1273:1292	The biological study	1273:1292	The biological study suggests that CPN3% composite may be a potential candidate as a wound healing material for biomedical application.
25780905	2	0	theme	microbial	354:362	arg1	FBMFC					375:379	FBMFC	375:379	FBMFC	375:379	This study reports a novel integration of HTL and carbon nanotubes (CNTs) fixed-bed microbial fuel cell (FBMFC) for continuous electricity generation from cornstalk biomass.
25780905	2	0	theme	microbial	354:362	arg1	cell					369:372	fixed-bed microbial fuel cell	344:372	fixed-bed microbial fuel cell (FBMFC)	344:380	This study reports a novel integration of HTL and carbon nanotubes (CNTs) fixed-bed microbial fuel cell (FBMFC) for continuous electricity generation from cornstalk biomass.
25780905	1	1	theme	chemicals	259:267	arg1	omission					237:244	the omission	233:244	the omission of hazardous chemicals	233:267	Hydrothermal liquefaction (HTL) is a green technology for biomass pretreatment with the omission of hazardous chemicals.
25780905	6	2	theme	biomass	1197:1203	arg1	degradation					1171:1181	degradation	1171:1181	degradation of cellulosic biomass	1171:1203	Distinguished from Proteobacteria, which a number of exoelectrogens belong to, the identified dominant genus Rhizobium in FBMFC was closely related to degradation of cellulosic biomass.
25780905	2	3	theme	fixed-bed	344:352	arg1	FBMFC					375:379	FBMFC	375:379	FBMFC	375:379	This study reports a novel integration of HTL and carbon nanotubes (CNTs) fixed-bed microbial fuel cell (FBMFC) for continuous electricity generation from cornstalk biomass.
25780905	2	3	theme	fixed-bed	344:352	arg1	cell					369:372	fixed-bed microbial fuel cell	344:372	fixed-bed microbial fuel cell (FBMFC)	344:380	This study reports a novel integration of HTL and carbon nanotubes (CNTs) fixed-bed microbial fuel cell (FBMFC) for continuous electricity generation from cornstalk biomass.
25780905	4	4	theme	Chemical	600:607	arg1	COD					624:626	COD	624:626	COD	624:626	About 80% of Chemical oxygen demand (COD) and Total organic carbon (TOC) was removed from low-Biochemical oxygen demand (BOD)/COD (0.16) cornstalk hydrolysate at 8.16g/L/d, whereas a maximum power density (680mW/m(3)) was obtained at 2.41g/L/d, and a smallest internal resistance (Rin) (28Ω) at 3.01g/L/d.
25780905	4	4	theme	Chemical	600:607	arg1	demand					616:621	Chemical oxygen demand	600:621	Chemical oxygen demand (COD)	600:627	About 80% of Chemical oxygen demand (COD) and Total organic carbon (TOC) was removed from low-Biochemical oxygen demand (BOD)/COD (0.16) cornstalk hydrolysate at 8.16g/L/d, whereas a maximum power density (680mW/m(3)) was obtained at 2.41g/L/d, and a smallest internal resistance (Rin) (28Ω) at 3.01g/L/d.
25780905	6	5	theme	cellulosic	1186:1195	arg1	biomass					1197:1203	cellulosic biomass	1186:1203	cellulosic biomass	1186:1203	Distinguished from Proteobacteria, which a number of exoelectrogens belong to, the identified dominant genus Rhizobium in FBMFC was closely related to degradation of cellulosic biomass.
25780905	4	6	dep	removed	664:670	arg1	whereas					760:766	whereas	760:766	whereas	760:766	About 80% of Chemical oxygen demand (COD) and Total organic carbon (TOC) was removed from low-Biochemical oxygen demand (BOD)/COD (0.16) cornstalk hydrolysate at 8.16g/L/d, whereas a maximum power density (680mW/m(3)) was obtained at 2.41g/L/d, and a smallest internal resistance (Rin) (28Ω) at 3.01g/L/d.
25780905	4	7	theme	internal	847:854	arg1	Rin					868:870	Rin	868:870	Rin	868:870	About 80% of Chemical oxygen demand (COD) and Total organic carbon (TOC) was removed from low-Biochemical oxygen demand (BOD)/COD (0.16) cornstalk hydrolysate at 8.16g/L/d, whereas a maximum power density (680mW/m(3)) was obtained at 2.41g/L/d, and a smallest internal resistance (Rin) (28Ω) at 3.01g/L/d.
25780905	4	7	theme	internal	847:854	arg1	28Ω					874:876	28Ω	874:876	28Ω	874:876	About 80% of Chemical oxygen demand (COD) and Total organic carbon (TOC) was removed from low-Biochemical oxygen demand (BOD)/COD (0.16) cornstalk hydrolysate at 8.16g/L/d, whereas a maximum power density (680mW/m(3)) was obtained at 2.41g/L/d, and a smallest internal resistance (Rin) (28Ω) at 3.01g/L/d.
25780905	4	7	theme	internal	847:854	arg1	resistance					856:865	a smallest internal resistance	836:865	a smallest internal resistance (Rin) (28Ω)	836:877	About 80% of Chemical oxygen demand (COD) and Total organic carbon (TOC) was removed from low-Biochemical oxygen demand (BOD)/COD (0.16) cornstalk hydrolysate at 8.16g/L/d, whereas a maximum power density (680mW/m(3)) was obtained at 2.41g/L/d, and a smallest internal resistance (Rin) (28Ω) at 3.01g/L/d.
25780905	4	8	theme	/COD	712:715	arg1	hydrolysate					734:744	low-Biochemical oxygen demand (BOD)/COD (0.16) cornstalk hydrolysate	677:744	low-Biochemical oxygen demand (BOD)/COD (0.16) cornstalk hydrolysate at 8.16g/L/d	677:757	About 80% of Chemical oxygen demand (COD) and Total organic carbon (TOC) was removed from low-Biochemical oxygen demand (BOD)/COD (0.16) cornstalk hydrolysate at 8.16g/L/d, whereas a maximum power density (680mW/m(3)) was obtained at 2.41g/L/d, and a smallest internal resistance (Rin) (28Ω) at 3.01g/L/d.
25780905	6	9	theme	dominant	1114:1121	arg1	related					1160:1166	related	1160:1166	related	1160:1166	Distinguished from Proteobacteria, which a number of exoelectrogens belong to, the identified dominant genus Rhizobium in FBMFC was closely related to degradation of cellulosic biomass.
25780905	6	9	theme	dominant	1114:1121	arg1	Rhizobium					1129:1137	the identified dominant genus Rhizobium	1099:1137	the identified dominant genus Rhizobium in FBMFC	1099:1146	Distinguished from Proteobacteria, which a number of exoelectrogens belong to, the identified dominant genus Rhizobium in FBMFC was closely related to degradation of cellulosic biomass.
25780905	2	10	theme	novel	291:295	arg1	integration					297:307	a novel integration	289:307	a novel integration of HTL and carbon nanotubes (CNTs) fixed-bed microbial fuel cell (FBMFC)	289:380	This study reports a novel integration of HTL and carbon nanotubes (CNTs) fixed-bed microbial fuel cell (FBMFC) for continuous electricity generation from cornstalk biomass.
25780905	1	11	theme	Hydrothermal	149:160	arg1	technology					192:201	a green technology	184:201	a green technology for biomass pretreatment with the omission of hazardous chemicals	184:267	Hydrothermal liquefaction (HTL) is a green technology for biomass pretreatment with the omission of hazardous chemicals.
25780905	1	11	theme	Hydrothermal	149:160	arg1	HTL					176:178	HTL	176:178	HTL	176:178	Hydrothermal liquefaction (HTL) is a green technology for biomass pretreatment with the omission of hazardous chemicals.
25780905	1	11	theme	Hydrothermal	149:160	arg1	liquefaction					162:173	Hydrothermal liquefaction	149:173	Hydrothermal liquefaction (HTL)	149:179	Hydrothermal liquefaction (HTL) is a green technology for biomass pretreatment with the omission of hazardous chemicals.
25780905	0	12	theme	liquefied	120:128	arg1	biomass					140:146	hydrothermal liquefied cornstalk biomass	107:146	hydrothermal liquefied cornstalk biomass	107:146	Performance and microbial community of carbon nanotube fixed-bed microbial fuel cell continuously fed with hydrothermal liquefied cornstalk biomass.
25780905	5	13	theme	cornstalk	997:1005	arg1	hydrolysate					1007:1017	cornstalk hydrolysate	997:1017	cornstalk hydrolysate	997:1017	Illumina MiSeq sequencing reveals the diverse microbial structure induced by the complex composition of cornstalk hydrolysate.
25780905	4	14	theme	demand	616:621	arg1	COD					624:626	COD	624:626	COD	624:626	About 80% of Chemical oxygen demand (COD) and Total organic carbon (TOC) was removed from low-Biochemical oxygen demand (BOD)/COD (0.16) cornstalk hydrolysate at 8.16g/L/d, whereas a maximum power density (680mW/m(3)) was obtained at 2.41g/L/d, and a smallest internal resistance (Rin) (28Ω) at 3.01g/L/d.
25780905	4	14	theme	demand	616:621	arg1	demand					616:621	Chemical oxygen demand	600:621	Chemical oxygen demand (COD)	600:627	About 80% of Chemical oxygen demand (COD) and Total organic carbon (TOC) was removed from low-Biochemical oxygen demand (BOD)/COD (0.16) cornstalk hydrolysate at 8.16g/L/d, whereas a maximum power density (680mW/m(3)) was obtained at 2.41g/L/d, and a smallest internal resistance (Rin) (28Ω) at 3.01g/L/d.
25780905	4	14	theme	demand	616:621	arg1	TOC					655:657	TOC	655:657	TOC	655:657	About 80% of Chemical oxygen demand (COD) and Total organic carbon (TOC) was removed from low-Biochemical oxygen demand (BOD)/COD (0.16) cornstalk hydrolysate at 8.16g/L/d, whereas a maximum power density (680mW/m(3)) was obtained at 2.41g/L/d, and a smallest internal resistance (Rin) (28Ω) at 3.01g/L/d.
25780905	4	14	theme	demand	616:621	arg1	%					595:595	About 80%	587:595	About 80% of Chemical oxygen demand (COD) and Total organic carbon (TOC)	587:658	About 80% of Chemical oxygen demand (COD) and Total organic carbon (TOC) was removed from low-Biochemical oxygen demand (BOD)/COD (0.16) cornstalk hydrolysate at 8.16g/L/d, whereas a maximum power density (680mW/m(3)) was obtained at 2.41g/L/d, and a smallest internal resistance (Rin) (28Ω) at 3.01g/L/d.
25780905	4	14	theme	demand	616:621	arg1	carbon					647:652	Total organic carbon	633:652	Total organic carbon (TOC)	633:658	About 80% of Chemical oxygen demand (COD) and Total organic carbon (TOC) was removed from low-Biochemical oxygen demand (BOD)/COD (0.16) cornstalk hydrolysate at 8.16g/L/d, whereas a maximum power density (680mW/m(3)) was obtained at 2.41g/L/d, and a smallest internal resistance (Rin) (28Ω) at 3.01g/L/d.
25780905	3	15	theme	organic	540:546	arg1	rates					556:560	different organic loading rates	530:560	different organic loading rates (OLRs) (0.82-8.16g/L/d)	530:584	Two FBMFCs in parallel achieved similar performance fed with cornstalk hydrolysate at different organic loading rates (OLRs) (0.82-8.16g/L/d).
25780905	3	15	theme	organic	540:546	arg1	OLRs					563:566	OLRs	563:566	OLRs	563:566	Two FBMFCs in parallel achieved similar performance fed with cornstalk hydrolysate at different organic loading rates (OLRs) (0.82-8.16g/L/d).
25780905	3	15	theme	organic	540:546	arg1	0.82-8.16g/L/d					570:583	0.82-8.16g/L/d	570:583	0.82-8.16g/L/d	570:583	Two FBMFCs in parallel achieved similar performance fed with cornstalk hydrolysate at different organic loading rates (OLRs) (0.82-8.16g/L/d).
25780905	4	16	theme	carbon	647:652	arg1	COD					624:626	COD	624:626	COD	624:626	About 80% of Chemical oxygen demand (COD) and Total organic carbon (TOC) was removed from low-Biochemical oxygen demand (BOD)/COD (0.16) cornstalk hydrolysate at 8.16g/L/d, whereas a maximum power density (680mW/m(3)) was obtained at 2.41g/L/d, and a smallest internal resistance (Rin) (28Ω) at 3.01g/L/d.
25780905	4	16	theme	carbon	647:652	arg1	demand					616:621	Chemical oxygen demand	600:621	Chemical oxygen demand (COD)	600:627	About 80% of Chemical oxygen demand (COD) and Total organic carbon (TOC) was removed from low-Biochemical oxygen demand (BOD)/COD (0.16) cornstalk hydrolysate at 8.16g/L/d, whereas a maximum power density (680mW/m(3)) was obtained at 2.41g/L/d, and a smallest internal resistance (Rin) (28Ω) at 3.01g/L/d.
25780905	4	16	theme	carbon	647:652	arg1	TOC					655:657	TOC	655:657	TOC	655:657	About 80% of Chemical oxygen demand (COD) and Total organic carbon (TOC) was removed from low-Biochemical oxygen demand (BOD)/COD (0.16) cornstalk hydrolysate at 8.16g/L/d, whereas a maximum power density (680mW/m(3)) was obtained at 2.41g/L/d, and a smallest internal resistance (Rin) (28Ω) at 3.01g/L/d.
25780905	4	16	theme	carbon	647:652	arg1	%					595:595	About 80%	587:595	About 80% of Chemical oxygen demand (COD) and Total organic carbon (TOC)	587:658	About 80% of Chemical oxygen demand (COD) and Total organic carbon (TOC) was removed from low-Biochemical oxygen demand (BOD)/COD (0.16) cornstalk hydrolysate at 8.16g/L/d, whereas a maximum power density (680mW/m(3)) was obtained at 2.41g/L/d, and a smallest internal resistance (Rin) (28Ω) at 3.01g/L/d.
25780905	4	16	theme	carbon	647:652	arg1	carbon					647:652	Total organic carbon	633:652	Total organic carbon (TOC)	633:658	About 80% of Chemical oxygen demand (COD) and Total organic carbon (TOC) was removed from low-Biochemical oxygen demand (BOD)/COD (0.16) cornstalk hydrolysate at 8.16g/L/d, whereas a maximum power density (680mW/m(3)) was obtained at 2.41g/L/d, and a smallest internal resistance (Rin) (28Ω) at 3.01g/L/d.
25780905	4	17	theme	Total	633:637	arg1	TOC					655:657	TOC	655:657	TOC	655:657	About 80% of Chemical oxygen demand (COD) and Total organic carbon (TOC) was removed from low-Biochemical oxygen demand (BOD)/COD (0.16) cornstalk hydrolysate at 8.16g/L/d, whereas a maximum power density (680mW/m(3)) was obtained at 2.41g/L/d, and a smallest internal resistance (Rin) (28Ω) at 3.01g/L/d.
25780905	4	17	theme	Total	633:637	arg1	carbon					647:652	Total organic carbon	633:652	Total organic carbon (TOC)	633:658	About 80% of Chemical oxygen demand (COD) and Total organic carbon (TOC) was removed from low-Biochemical oxygen demand (BOD)/COD (0.16) cornstalk hydrolysate at 8.16g/L/d, whereas a maximum power density (680mW/m(3)) was obtained at 2.41g/L/d, and a smallest internal resistance (Rin) (28Ω) at 3.01g/L/d.
25780905	0	18	theme	hydrothermal	107:118	arg1	biomass					140:146	hydrothermal liquefied cornstalk biomass	107:146	hydrothermal liquefied cornstalk biomass	107:146	Performance and microbial community of carbon nanotube fixed-bed microbial fuel cell continuously fed with hydrothermal liquefied cornstalk biomass.
25780905	5	19	theme	hydrolysate	1007:1017	arg1	composition					982:992	the complex composition	970:992	the complex composition of cornstalk hydrolysate	970:1017	Illumina MiSeq sequencing reveals the diverse microbial structure induced by the complex composition of cornstalk hydrolysate.
25780905	4	20	theme	power	778:782	arg1	680mW/m					793:799	680mW/m(3)	793:802	680mW/m(3)	793:802	About 80% of Chemical oxygen demand (COD) and Total organic carbon (TOC) was removed from low-Biochemical oxygen demand (BOD)/COD (0.16) cornstalk hydrolysate at 8.16g/L/d, whereas a maximum power density (680mW/m(3)) was obtained at 2.41g/L/d, and a smallest internal resistance (Rin) (28Ω) at 3.01g/L/d.
25780905	4	20	theme	power	778:782	arg1	density					784:790	a maximum power density	768:790	a maximum power density (680mW/m(3))	768:803	About 80% of Chemical oxygen demand (COD) and Total organic carbon (TOC) was removed from low-Biochemical oxygen demand (BOD)/COD (0.16) cornstalk hydrolysate at 8.16g/L/d, whereas a maximum power density (680mW/m(3)) was obtained at 2.41g/L/d, and a smallest internal resistance (Rin) (28Ω) at 3.01g/L/d.
25780905	3	21	theme	loading	548:554	arg1	rates					556:560	different organic loading rates	530:560	different organic loading rates (OLRs) (0.82-8.16g/L/d)	530:584	Two FBMFCs in parallel achieved similar performance fed with cornstalk hydrolysate at different organic loading rates (OLRs) (0.82-8.16g/L/d).
25780905	3	21	theme	loading	548:554	arg1	OLRs					563:566	OLRs	563:566	OLRs	563:566	Two FBMFCs in parallel achieved similar performance fed with cornstalk hydrolysate at different organic loading rates (OLRs) (0.82-8.16g/L/d).
25780905	3	21	theme	loading	548:554	arg1	0.82-8.16g/L/d					570:583	0.82-8.16g/L/d	570:583	0.82-8.16g/L/d	570:583	Two FBMFCs in parallel achieved similar performance fed with cornstalk hydrolysate at different organic loading rates (OLRs) (0.82-8.16g/L/d).
25780905	3	22	theme	different	530:538	arg1	rates					556:560	different organic loading rates	530:560	different organic loading rates (OLRs) (0.82-8.16g/L/d)	530:584	Two FBMFCs in parallel achieved similar performance fed with cornstalk hydrolysate at different organic loading rates (OLRs) (0.82-8.16g/L/d).
25780905	3	22	theme	different	530:538	arg1	OLRs					563:566	OLRs	563:566	OLRs	563:566	Two FBMFCs in parallel achieved similar performance fed with cornstalk hydrolysate at different organic loading rates (OLRs) (0.82-8.16g/L/d).
25780905	3	22	theme	different	530:538	arg1	0.82-8.16g/L/d					570:583	0.82-8.16g/L/d	570:583	0.82-8.16g/L/d	570:583	Two FBMFCs in parallel achieved similar performance fed with cornstalk hydrolysate at different organic loading rates (OLRs) (0.82-8.16g/L/d).
25780905	2	23	theme	carbon	320:325	arg1	CNTs					338:341	CNTs	338:341	CNTs	338:341	This study reports a novel integration of HTL and carbon nanotubes (CNTs) fixed-bed microbial fuel cell (FBMFC) for continuous electricity generation from cornstalk biomass.
25780905	2	23	theme	carbon	320:325	arg1	nanotubes					327:335	carbon nanotubes	320:335	carbon nanotubes (CNTs)	320:342	This study reports a novel integration of HTL and carbon nanotubes (CNTs) fixed-bed microbial fuel cell (FBMFC) for continuous electricity generation from cornstalk biomass.
25780905	1	24	theme	green	186:190	arg1	technology					192:201	a green technology	184:201	a green technology for biomass pretreatment with the omission of hazardous chemicals	184:267	Hydrothermal liquefaction (HTL) is a green technology for biomass pretreatment with the omission of hazardous chemicals.
25780905	1	24	theme	green	186:190	arg1	liquefaction					162:173	Hydrothermal liquefaction	149:173	Hydrothermal liquefaction (HTL)	149:179	Hydrothermal liquefaction (HTL) is a green technology for biomass pretreatment with the omission of hazardous chemicals.
25780905	4	25	theme	low-Biochemical	677:691	arg1	hydrolysate					734:744	low-Biochemical oxygen demand (BOD)/COD (0.16) cornstalk hydrolysate	677:744	low-Biochemical oxygen demand (BOD)/COD (0.16) cornstalk hydrolysate at 8.16g/L/d	677:757	About 80% of Chemical oxygen demand (COD) and Total organic carbon (TOC) was removed from low-Biochemical oxygen demand (BOD)/COD (0.16) cornstalk hydrolysate at 8.16g/L/d, whereas a maximum power density (680mW/m(3)) was obtained at 2.41g/L/d, and a smallest internal resistance (Rin) (28Ω) at 3.01g/L/d.
25780905	4	26	theme	demand	700:705	arg1	hydrolysate					734:744	low-Biochemical oxygen demand (BOD)/COD (0.16) cornstalk hydrolysate	677:744	low-Biochemical oxygen demand (BOD)/COD (0.16) cornstalk hydrolysate at 8.16g/L/d	677:757	About 80% of Chemical oxygen demand (COD) and Total organic carbon (TOC) was removed from low-Biochemical oxygen demand (BOD)/COD (0.16) cornstalk hydrolysate at 8.16g/L/d, whereas a maximum power density (680mW/m(3)) was obtained at 2.41g/L/d, and a smallest internal resistance (Rin) (28Ω) at 3.01g/L/d.
25780905	4	27	theme	organic	639:645	arg1	TOC					655:657	TOC	655:657	TOC	655:657	About 80% of Chemical oxygen demand (COD) and Total organic carbon (TOC) was removed from low-Biochemical oxygen demand (BOD)/COD (0.16) cornstalk hydrolysate at 8.16g/L/d, whereas a maximum power density (680mW/m(3)) was obtained at 2.41g/L/d, and a smallest internal resistance (Rin) (28Ω) at 3.01g/L/d.
25780905	4	27	theme	organic	639:645	arg1	carbon					647:652	Total organic carbon	633:652	Total organic carbon (TOC)	633:658	About 80% of Chemical oxygen demand (COD) and Total organic carbon (TOC) was removed from low-Biochemical oxygen demand (BOD)/COD (0.16) cornstalk hydrolysate at 8.16g/L/d, whereas a maximum power density (680mW/m(3)) was obtained at 2.41g/L/d, and a smallest internal resistance (Rin) (28Ω) at 3.01g/L/d.
25780905	1	28	with	pretreatment	215:226	arg1	omission					237:244	the omission	233:244	the omission of hazardous chemicals	233:267	Hydrothermal liquefaction (HTL) is a green technology for biomass pretreatment with the omission of hazardous chemicals.
25780905	2	29	theme	HTL	312:314	arg1	integration					297:307	a novel integration	289:307	a novel integration of HTL and carbon nanotubes (CNTs) fixed-bed microbial fuel cell (FBMFC)	289:380	This study reports a novel integration of HTL and carbon nanotubes (CNTs) fixed-bed microbial fuel cell (FBMFC) for continuous electricity generation from cornstalk biomass.
25780905	2	30	theme	cornstalk	425:433	arg1	biomass					435:441	cornstalk biomass	425:441	cornstalk biomass	425:441	This study reports a novel integration of HTL and carbon nanotubes (CNTs) fixed-bed microbial fuel cell (FBMFC) for continuous electricity generation from cornstalk biomass.
25780905	0	31	theme	cornstalk	130:138	arg1	biomass					140:146	hydrothermal liquefied cornstalk biomass	107:146	hydrothermal liquefied cornstalk biomass	107:146	Performance and microbial community of carbon nanotube fixed-bed microbial fuel cell continuously fed with hydrothermal liquefied cornstalk biomass.
25780905	4	32	theme	BOD	708:710	arg1	hydrolysate					734:744	low-Biochemical oxygen demand (BOD)/COD (0.16) cornstalk hydrolysate	677:744	low-Biochemical oxygen demand (BOD)/COD (0.16) cornstalk hydrolysate at 8.16g/L/d	677:757	About 80% of Chemical oxygen demand (COD) and Total organic carbon (TOC) was removed from low-Biochemical oxygen demand (BOD)/COD (0.16) cornstalk hydrolysate at 8.16g/L/d, whereas a maximum power density (680mW/m(3)) was obtained at 2.41g/L/d, and a smallest internal resistance (Rin) (28Ω) at 3.01g/L/d.
25780905	1	33	theme	biomass	207:213	arg1	pretreatment					215:226	biomass pretreatment	207:226	biomass pretreatment with the omission of hazardous chemicals	207:267	Hydrothermal liquefaction (HTL) is a green technology for biomass pretreatment with the omission of hazardous chemicals.
25780905	0	34	theme	carbon	39:44	arg1	Performance					0:10	Performance	0:10	Performance	0:10	Performance and microbial community of carbon nanotube fixed-bed microbial fuel cell continuously fed with hydrothermal liquefied cornstalk biomass.
25780905	0	34	theme	carbon	39:44	arg1	community					26:34	microbial community	16:34	microbial community	16:34	Performance and microbial community of carbon nanotube fixed-bed microbial fuel cell continuously fed with hydrothermal liquefied cornstalk biomass.
25780905	4	35	theme	smallest	838:845	arg1	Rin					868:870	Rin	868:870	Rin	868:870	About 80% of Chemical oxygen demand (COD) and Total organic carbon (TOC) was removed from low-Biochemical oxygen demand (BOD)/COD (0.16) cornstalk hydrolysate at 8.16g/L/d, whereas a maximum power density (680mW/m(3)) was obtained at 2.41g/L/d, and a smallest internal resistance (Rin) (28Ω) at 3.01g/L/d.
25780905	4	35	theme	smallest	838:845	arg1	28Ω					874:876	28Ω	874:876	28Ω	874:876	About 80% of Chemical oxygen demand (COD) and Total organic carbon (TOC) was removed from low-Biochemical oxygen demand (BOD)/COD (0.16) cornstalk hydrolysate at 8.16g/L/d, whereas a maximum power density (680mW/m(3)) was obtained at 2.41g/L/d, and a smallest internal resistance (Rin) (28Ω) at 3.01g/L/d.
25780905	4	35	theme	smallest	838:845	arg1	resistance					856:865	a smallest internal resistance	836:865	a smallest internal resistance (Rin) (28Ω)	836:877	About 80% of Chemical oxygen demand (COD) and Total organic carbon (TOC) was removed from low-Biochemical oxygen demand (BOD)/COD (0.16) cornstalk hydrolysate at 8.16g/L/d, whereas a maximum power density (680mW/m(3)) was obtained at 2.41g/L/d, and a smallest internal resistance (Rin) (28Ω) at 3.01g/L/d.
25780905	5	36	theme	MiSeq	902:906	arg1	sequencing					908:917	Illumina MiSeq sequencing	893:917	Illumina MiSeq sequencing	893:917	Illumina MiSeq sequencing reveals the diverse microbial structure induced by the complex composition of cornstalk hydrolysate.
25780905	2	37	theme	electricity	397:407	arg1	generation					409:418	continuous electricity generation	386:418	continuous electricity generation from cornstalk biomass	386:441	This study reports a novel integration of HTL and carbon nanotubes (CNTs) fixed-bed microbial fuel cell (FBMFC) for continuous electricity generation from cornstalk biomass.
25780905	5	38	theme	complex	974:980	arg1	composition					982:992	the complex composition	970:992	the complex composition of cornstalk hydrolysate	970:1017	Illumina MiSeq sequencing reveals the diverse microbial structure induced by the complex composition of cornstalk hydrolysate.
25780905	3	39	from	FBMFCs	448:453	arg1	parallel					458:465	parallel	458:465	parallel	458:465	Two FBMFCs in parallel achieved similar performance fed with cornstalk hydrolysate at different organic loading rates (OLRs) (0.82-8.16g/L/d).
25780905	2	40	theme	continuous	386:395	arg1	generation					409:418	continuous electricity generation	386:418	continuous electricity generation from cornstalk biomass	386:441	This study reports a novel integration of HTL and carbon nanotubes (CNTs) fixed-bed microbial fuel cell (FBMFC) for continuous electricity generation from cornstalk biomass.
25780905	0	41	theme	fixed-bed	55:63	arg1	cell					80:83	fixed-bed microbial fuel cell	55:83	fixed-bed microbial fuel cell continuously fed with hydrothermal liquefied cornstalk biomass	55:146	Performance and microbial community of carbon nanotube fixed-bed microbial fuel cell continuously fed with hydrothermal liquefied cornstalk biomass.
25780905	3	42	theme	cornstalk	505:513	arg1	hydrolysate					515:525	cornstalk hydrolysate	505:525	cornstalk hydrolysate	505:525	Two FBMFCs in parallel achieved similar performance fed with cornstalk hydrolysate at different organic loading rates (OLRs) (0.82-8.16g/L/d).
25780905	4	43	theme	maximum	770:776	arg1	680mW/m					793:799	680mW/m(3)	793:802	680mW/m(3)	793:802	About 80% of Chemical oxygen demand (COD) and Total organic carbon (TOC) was removed from low-Biochemical oxygen demand (BOD)/COD (0.16) cornstalk hydrolysate at 8.16g/L/d, whereas a maximum power density (680mW/m(3)) was obtained at 2.41g/L/d, and a smallest internal resistance (Rin) (28Ω) at 3.01g/L/d.
25780905	4	43	theme	maximum	770:776	arg1	density					784:790	a maximum power density	768:790	a maximum power density (680mW/m(3))	768:803	About 80% of Chemical oxygen demand (COD) and Total organic carbon (TOC) was removed from low-Biochemical oxygen demand (BOD)/COD (0.16) cornstalk hydrolysate at 8.16g/L/d, whereas a maximum power density (680mW/m(3)) was obtained at 2.41g/L/d, and a smallest internal resistance (Rin) (28Ω) at 3.01g/L/d.
25780905	2	44	dep	HTL	312:314	arg1	FBMFC					375:379	FBMFC	375:379	FBMFC	375:379	This study reports a novel integration of HTL and carbon nanotubes (CNTs) fixed-bed microbial fuel cell (FBMFC) for continuous electricity generation from cornstalk biomass.
25780905	2	44	dep	HTL	312:314	arg1	cell					369:372	fixed-bed microbial fuel cell	344:372	fixed-bed microbial fuel cell (FBMFC)	344:380	This study reports a novel integration of HTL and carbon nanotubes (CNTs) fixed-bed microbial fuel cell (FBMFC) for continuous electricity generation from cornstalk biomass.
25780905	4	45	attach	removed	664:670	arg2	carbon					647:652	Total organic carbon	633:652	Total organic carbon (TOC)	633:658	About 80% of Chemical oxygen demand (COD) and Total organic carbon (TOC) was removed from low-Biochemical oxygen demand (BOD)/COD (0.16) cornstalk hydrolysate at 8.16g/L/d, whereas a maximum power density (680mW/m(3)) was obtained at 2.41g/L/d, and a smallest internal resistance (Rin) (28Ω) at 3.01g/L/d.
25780905	4	45	attach	removed	664:670	arg1	hydrolysate					734:744	low-Biochemical oxygen demand (BOD)/COD (0.16) cornstalk hydrolysate	677:744	low-Biochemical oxygen demand (BOD)/COD (0.16) cornstalk hydrolysate at 8.16g/L/d	677:757	About 80% of Chemical oxygen demand (COD) and Total organic carbon (TOC) was removed from low-Biochemical oxygen demand (BOD)/COD (0.16) cornstalk hydrolysate at 8.16g/L/d, whereas a maximum power density (680mW/m(3)) was obtained at 2.41g/L/d, and a smallest internal resistance (Rin) (28Ω) at 3.01g/L/d.
25780905	4	45	attach	removed	664:670	arg2	demand					616:621	Chemical oxygen demand	600:621	Chemical oxygen demand (COD)	600:627	About 80% of Chemical oxygen demand (COD) and Total organic carbon (TOC) was removed from low-Biochemical oxygen demand (BOD)/COD (0.16) cornstalk hydrolysate at 8.16g/L/d, whereas a maximum power density (680mW/m(3)) was obtained at 2.41g/L/d, and a smallest internal resistance (Rin) (28Ω) at 3.01g/L/d.
25780905	4	45	attach	removed	664:670	arg2	%					595:595	About 80%	587:595	About 80% of Chemical oxygen demand (COD) and Total organic carbon (TOC)	587:658	About 80% of Chemical oxygen demand (COD) and Total organic carbon (TOC) was removed from low-Biochemical oxygen demand (BOD)/COD (0.16) cornstalk hydrolysate at 8.16g/L/d, whereas a maximum power density (680mW/m(3)) was obtained at 2.41g/L/d, and a smallest internal resistance (Rin) (28Ω) at 3.01g/L/d.
25780905	4	45	attach	removed	664:670	arg2	TOC					655:657	TOC	655:657	TOC	655:657	About 80% of Chemical oxygen demand (COD) and Total organic carbon (TOC) was removed from low-Biochemical oxygen demand (BOD)/COD (0.16) cornstalk hydrolysate at 8.16g/L/d, whereas a maximum power density (680mW/m(3)) was obtained at 2.41g/L/d, and a smallest internal resistance (Rin) (28Ω) at 3.01g/L/d.
25780905	4	45	attach	removed	664:670	arg2	COD					624:626	COD	624:626	COD	624:626	About 80% of Chemical oxygen demand (COD) and Total organic carbon (TOC) was removed from low-Biochemical oxygen demand (BOD)/COD (0.16) cornstalk hydrolysate at 8.16g/L/d, whereas a maximum power density (680mW/m(3)) was obtained at 2.41g/L/d, and a smallest internal resistance (Rin) (28Ω) at 3.01g/L/d.
25780905	5	46	theme	diverse	931:937	arg1	structure					949:957	the diverse microbial structure	927:957	the diverse microbial structure induced by the complex composition of cornstalk hydrolysate	927:1017	Illumina MiSeq sequencing reveals the diverse microbial structure induced by the complex composition of cornstalk hydrolysate.
25780905	5	47	theme	Illumina	893:900	arg1	sequencing					908:917	Illumina MiSeq sequencing	893:917	Illumina MiSeq sequencing	893:917	Illumina MiSeq sequencing reveals the diverse microbial structure induced by the complex composition of cornstalk hydrolysate.
25780905	5	48	theme	microbial	939:947	arg1	structure					949:957	the diverse microbial structure	927:957	the diverse microbial structure induced by the complex composition of cornstalk hydrolysate	927:1017	Illumina MiSeq sequencing reveals the diverse microbial structure induced by the complex composition of cornstalk hydrolysate.
25780905	6	49	theme	genus	1123:1127	arg1	related					1160:1166	related	1160:1166	related	1160:1166	Distinguished from Proteobacteria, which a number of exoelectrogens belong to, the identified dominant genus Rhizobium in FBMFC was closely related to degradation of cellulosic biomass.
25780905	6	49	theme	genus	1123:1127	arg1	Rhizobium					1129:1137	the identified dominant genus Rhizobium	1099:1137	the identified dominant genus Rhizobium in FBMFC	1099:1146	Distinguished from Proteobacteria, which a number of exoelectrogens belong to, the identified dominant genus Rhizobium in FBMFC was closely related to degradation of cellulosic biomass.
25780905	3	50	theme	similar	476:482	arg1	performance					484:494	similar performance	476:494	similar performance fed with cornstalk hydrolysate at different organic loading rates (OLRs) (0.82-8.16g/L/d)	476:584	Two FBMFCs in parallel achieved similar performance fed with cornstalk hydrolysate at different organic loading rates (OLRs) (0.82-8.16g/L/d).
25780905	6	51	from	Rhizobium	1129:1137	arg1	FBMFC					1142:1146	FBMFC	1142:1146	FBMFC	1142:1146	Distinguished from Proteobacteria, which a number of exoelectrogens belong to, the identified dominant genus Rhizobium in FBMFC was closely related to degradation of cellulosic biomass.
25780905	6	52	theme	exoelectrogens	1073:1086	arg1	number					1063:1068	a number	1061:1068	a number of exoelectrogens	1061:1086	Distinguished from Proteobacteria, which a number of exoelectrogens belong to, the identified dominant genus Rhizobium in FBMFC was closely related to degradation of cellulosic biomass.
25780905	4	53	theme	cornstalk	724:732	arg1	hydrolysate					734:744	low-Biochemical oxygen demand (BOD)/COD (0.16) cornstalk hydrolysate	677:744	low-Biochemical oxygen demand (BOD)/COD (0.16) cornstalk hydrolysate at 8.16g/L/d	677:757	About 80% of Chemical oxygen demand (COD) and Total organic carbon (TOC) was removed from low-Biochemical oxygen demand (BOD)/COD (0.16) cornstalk hydrolysate at 8.16g/L/d, whereas a maximum power density (680mW/m(3)) was obtained at 2.41g/L/d, and a smallest internal resistance (Rin) (28Ω) at 3.01g/L/d.
25780905	4	54	from	8.16g/L/d	749:757	arg1	hydrolysate					734:744	low-Biochemical oxygen demand (BOD)/COD (0.16) cornstalk hydrolysate	677:744	low-Biochemical oxygen demand (BOD)/COD (0.16) cornstalk hydrolysate at 8.16g/L/d	677:757	About 80% of Chemical oxygen demand (COD) and Total organic carbon (TOC) was removed from low-Biochemical oxygen demand (BOD)/COD (0.16) cornstalk hydrolysate at 8.16g/L/d, whereas a maximum power density (680mW/m(3)) was obtained at 2.41g/L/d, and a smallest internal resistance (Rin) (28Ω) at 3.01g/L/d.
25780905	2	55	theme	nanotubes	327:335	arg1	integration					297:307	a novel integration	289:307	a novel integration of HTL and carbon nanotubes (CNTs) fixed-bed microbial fuel cell (FBMFC)	289:380	This study reports a novel integration of HTL and carbon nanotubes (CNTs) fixed-bed microbial fuel cell (FBMFC) for continuous electricity generation from cornstalk biomass.
25780905	4	56	theme	oxygen	693:698	arg1	hydrolysate					734:744	low-Biochemical oxygen demand (BOD)/COD (0.16) cornstalk hydrolysate	677:744	low-Biochemical oxygen demand (BOD)/COD (0.16) cornstalk hydrolysate at 8.16g/L/d	677:757	About 80% of Chemical oxygen demand (COD) and Total organic carbon (TOC) was removed from low-Biochemical oxygen demand (BOD)/COD (0.16) cornstalk hydrolysate at 8.16g/L/d, whereas a maximum power density (680mW/m(3)) was obtained at 2.41g/L/d, and a smallest internal resistance (Rin) (28Ω) at 3.01g/L/d.
25780905	0	57	theme	fuel	75:78	arg1	cell					80:83	fixed-bed microbial fuel cell	55:83	fixed-bed microbial fuel cell continuously fed with hydrothermal liquefied cornstalk biomass	55:146	Performance and microbial community of carbon nanotube fixed-bed microbial fuel cell continuously fed with hydrothermal liquefied cornstalk biomass.
25780905	6	58	theme	identified	1103:1112	arg1	related					1160:1166	related	1160:1166	related	1160:1166	Distinguished from Proteobacteria, which a number of exoelectrogens belong to, the identified dominant genus Rhizobium in FBMFC was closely related to degradation of cellulosic biomass.
25780905	6	58	theme	identified	1103:1112	arg1	Rhizobium					1129:1137	the identified dominant genus Rhizobium	1099:1137	the identified dominant genus Rhizobium in FBMFC	1099:1146	Distinguished from Proteobacteria, which a number of exoelectrogens belong to, the identified dominant genus Rhizobium in FBMFC was closely related to degradation of cellulosic biomass.
25780905	4	59	theme	oxygen	609:614	arg1	COD					624:626	COD	624:626	COD	624:626	About 80% of Chemical oxygen demand (COD) and Total organic carbon (TOC) was removed from low-Biochemical oxygen demand (BOD)/COD (0.16) cornstalk hydrolysate at 8.16g/L/d, whereas a maximum power density (680mW/m(3)) was obtained at 2.41g/L/d, and a smallest internal resistance (Rin) (28Ω) at 3.01g/L/d.
25780905	4	59	theme	oxygen	609:614	arg1	demand					616:621	Chemical oxygen demand	600:621	Chemical oxygen demand (COD)	600:627	About 80% of Chemical oxygen demand (COD) and Total organic carbon (TOC) was removed from low-Biochemical oxygen demand (BOD)/COD (0.16) cornstalk hydrolysate at 8.16g/L/d, whereas a maximum power density (680mW/m(3)) was obtained at 2.41g/L/d, and a smallest internal resistance (Rin) (28Ω) at 3.01g/L/d.
25780905	0	60	theme	microbial	65:73	arg1	cell					80:83	fixed-bed microbial fuel cell	55:83	fixed-bed microbial fuel cell continuously fed with hydrothermal liquefied cornstalk biomass	55:146	Performance and microbial community of carbon nanotube fixed-bed microbial fuel cell continuously fed with hydrothermal liquefied cornstalk biomass.
25780905	2	61	from	biomass	435:441	arg1	generation					409:418	continuous electricity generation	386:418	continuous electricity generation from cornstalk biomass	386:441	This study reports a novel integration of HTL and carbon nanotubes (CNTs) fixed-bed microbial fuel cell (FBMFC) for continuous electricity generation from cornstalk biomass.
25780905	0	62	theme	microbial	16:24	arg1	community					26:34	microbial community	16:34	microbial community	16:34	Performance and microbial community of carbon nanotube fixed-bed microbial fuel cell continuously fed with hydrothermal liquefied cornstalk biomass.
25780905	2	63	theme	fuel	364:367	arg1	FBMFC					375:379	FBMFC	375:379	FBMFC	375:379	This study reports a novel integration of HTL and carbon nanotubes (CNTs) fixed-bed microbial fuel cell (FBMFC) for continuous electricity generation from cornstalk biomass.
25780905	2	63	theme	fuel	364:367	arg1	cell					369:372	fixed-bed microbial fuel cell	344:372	fixed-bed microbial fuel cell (FBMFC)	344:380	This study reports a novel integration of HTL and carbon nanotubes (CNTs) fixed-bed microbial fuel cell (FBMFC) for continuous electricity generation from cornstalk biomass.
25780905	1	64	theme	hazardous	249:257	arg1	chemicals					259:267	hazardous chemicals	249:267	hazardous chemicals	249:267	Hydrothermal liquefaction (HTL) is a green technology for biomass pretreatment with the omission of hazardous chemicals.
25062502	3	0	from	ratio	739:743	arg1	incorporation					695:707	the incorporation	691:707	the incorporation of CNF preferably at a weight ratio of 30 wt %	691:754	Results show that the incorporation of CNF preferably at a weight ratio of 30 wt % greatly enhanced the mechanical strength and modulus, energy absorption, water resistance, and dimensional stability of the composite foams because of the rigid and semicrystalline nature of CNF as well as regular and compact pore structures.
25062502	5	1	theme	freeze-drying	1350:1362	arg1	process					1364:1370	the freeze-drying process	1346:1370	the freeze-drying process	1346:1370	Hence, we provided some meaningful information on the biomimetic cellular composite foams with controllable morphs and properties by varying the freeze-drying process and composition.
25062502	3	2	theme	wt	751:752	arg1	%					754:754	30 wt %	748:754	30 wt %	748:754	Results show that the incorporation of CNF preferably at a weight ratio of 30 wt % greatly enhanced the mechanical strength and modulus, energy absorption, water resistance, and dimensional stability of the composite foams because of the rigid and semicrystalline nature of CNF as well as regular and compact pore structures.
25062502	3	3	theme	compact	974:980	arg1	structures					987:996	regular and compact pore structures	962:996	regular and compact pore structures	962:996	Results show that the incorporation of CNF preferably at a weight ratio of 30 wt % greatly enhanced the mechanical strength and modulus, energy absorption, water resistance, and dimensional stability of the composite foams because of the rigid and semicrystalline nature of CNF as well as regular and compact pore structures.
25062502	3	4	theme	semicrystalline	921:935	arg1	nature					937:942	the rigid and semicrystalline nature	907:942	the rigid and semicrystalline nature of CNF	907:949	Results show that the incorporation of CNF preferably at a weight ratio of 30 wt % greatly enhanced the mechanical strength and modulus, energy absorption, water resistance, and dimensional stability of the composite foams because of the rigid and semicrystalline nature of CNF as well as regular and compact pore structures.
25062502	3	5	theme	regular	962:968	arg1	structures					987:996	regular and compact pore structures	962:996	regular and compact pore structures	962:996	Results show that the incorporation of CNF preferably at a weight ratio of 30 wt % greatly enhanced the mechanical strength and modulus, energy absorption, water resistance, and dimensional stability of the composite foams because of the rigid and semicrystalline nature of CNF as well as regular and compact pore structures.
25062502	1	6	theme	vinyl	228:232	arg1	poly					223:226	poly	223:226	poly(vinyl alcohol) (PVA) supported by cellulose nanofibrils (CNF)	223:288	In order to capture savings in energy and ambitious environmental targets, biodegradable composite foams of poly(vinyl alcohol) (PVA) supported by cellulose nanofibrils (CNF) were prepared through unidirectional freeze-drying technology.
25062502	1	6	theme	vinyl	228:232	arg1	alcohol					234:240	vinyl alcohol	228:240	vinyl alcohol	228:240	In order to capture savings in energy and ambitious environmental targets, biodegradable composite foams of poly(vinyl alcohol) (PVA) supported by cellulose nanofibrils (CNF) were prepared through unidirectional freeze-drying technology.
25062502	4	7	theme	CNF	1124:1126	arg1	involvement					1109:1119	the involvement	1105:1119	the involvement of CNF	1105:1126	Furthermore, the biodegradation tests performed in a simulated aerobic compost environment suggested that the involvement of CNF significantly accelerated the pace of biodegradation of the composite foams.
25062502	2	8	theme	electron	551:558	arg1	microscopy					560:569	scanning electron microscopy	542:569	scanning electron microscopy (SEM)	542:575	Effects of the content of CNF, the solid content of the precursor suspension, and the quenching temperature on the microstructure and properties of the composite foams were investigated by scanning electron microscopy (SEM), compressive testing, X-ray diffraction (XRD) analysis, water uptake, and biodegradation tests.
25062502	2	8	theme	electron	551:558	arg1	XRD					618:620	XRD	618:620	XRD	618:620	Effects of the content of CNF, the solid content of the precursor suspension, and the quenching temperature on the microstructure and properties of the composite foams were investigated by scanning electron microscopy (SEM), compressive testing, X-ray diffraction (XRD) analysis, water uptake, and biodegradation tests.
25062502	2	8	theme	electron	551:558	arg1	SEM					572:574	SEM	572:574	SEM	572:574	Effects of the content of CNF, the solid content of the precursor suspension, and the quenching temperature on the microstructure and properties of the composite foams were investigated by scanning electron microscopy (SEM), compressive testing, X-ray diffraction (XRD) analysis, water uptake, and biodegradation tests.
25062502	1	9	from	savings	135:141	arg1	targets					181:187	ambitious environmental targets	157:187	ambitious environmental targets	157:187	In order to capture savings in energy and ambitious environmental targets, biodegradable composite foams of poly(vinyl alcohol) (PVA) supported by cellulose nanofibrils (CNF) were prepared through unidirectional freeze-drying technology.
25062502	1	9	from	savings	135:141	arg1	energy					146:151	energy	146:151	energy	146:151	In order to capture savings in energy and ambitious environmental targets, biodegradable composite foams of poly(vinyl alcohol) (PVA) supported by cellulose nanofibrils (CNF) were prepared through unidirectional freeze-drying technology.
25062502	5	10	theme	cellular	1270:1277	arg1	foams					1289:1293	the biomimetic cellular composite foams	1255:1293	the biomimetic cellular composite foams with controllable morphs and properties by varying the freeze-drying process and composition	1255:1386	Hence, we provided some meaningful information on the biomimetic cellular composite foams with controllable morphs and properties by varying the freeze-drying process and composition.
25062502	2	11	theme	scanning	542:549	arg1	microscopy					560:569	scanning electron microscopy	542:569	scanning electron microscopy (SEM)	542:575	Effects of the content of CNF, the solid content of the precursor suspension, and the quenching temperature on the microstructure and properties of the composite foams were investigated by scanning electron microscopy (SEM), compressive testing, X-ray diffraction (XRD) analysis, water uptake, and biodegradation tests.
25062502	2	11	theme	scanning	542:549	arg1	XRD					618:620	XRD	618:620	XRD	618:620	Effects of the content of CNF, the solid content of the precursor suspension, and the quenching temperature on the microstructure and properties of the composite foams were investigated by scanning electron microscopy (SEM), compressive testing, X-ray diffraction (XRD) analysis, water uptake, and biodegradation tests.
25062502	2	11	theme	scanning	542:549	arg1	SEM					572:574	SEM	572:574	SEM	572:574	Effects of the content of CNF, the solid content of the precursor suspension, and the quenching temperature on the microstructure and properties of the composite foams were investigated by scanning electron microscopy (SEM), compressive testing, X-ray diffraction (XRD) analysis, water uptake, and biodegradation tests.
25062502	3	12	theme	rigid	911:915	arg1	nature					937:942	the rigid and semicrystalline nature	907:942	the rigid and semicrystalline nature of CNF	907:949	Results show that the incorporation of CNF preferably at a weight ratio of 30 wt % greatly enhanced the mechanical strength and modulus, energy absorption, water resistance, and dimensional stability of the composite foams because of the rigid and semicrystalline nature of CNF as well as regular and compact pore structures.
25062502	1	13	theme	unidirectional	312:325	arg1	technology					341:350	unidirectional freeze-drying technology	312:350	unidirectional freeze-drying technology	312:350	In order to capture savings in energy and ambitious environmental targets, biodegradable composite foams of poly(vinyl alcohol) (PVA) supported by cellulose nanofibrils (CNF) were prepared through unidirectional freeze-drying technology.
25062502	2	14	theme	compressive	578:588	arg1	testing					590:596	compressive testing	578:596	compressive testing	578:596	Effects of the content of CNF, the solid content of the precursor suspension, and the quenching temperature on the microstructure and properties of the composite foams were investigated by scanning electron microscopy (SEM), compressive testing, X-ray diffraction (XRD) analysis, water uptake, and biodegradation tests.
25062502	4	15	theme	biodegradation	1166:1179	arg1	pace					1158:1161	the pace	1154:1161	the pace of biodegradation of the composite foams	1154:1202	Furthermore, the biodegradation tests performed in a simulated aerobic compost environment suggested that the involvement of CNF significantly accelerated the pace of biodegradation of the composite foams.
25062502	3	16	theme	water	829:833	arg1	resistance					835:844	water resistance	829:844	water resistance	829:844	Results show that the incorporation of CNF preferably at a weight ratio of 30 wt % greatly enhanced the mechanical strength and modulus, energy absorption, water resistance, and dimensional stability of the composite foams because of the rigid and semicrystalline nature of CNF as well as regular and compact pore structures.
25062502	1	17	theme	freeze-drying	327:339	arg1	technology					341:350	unidirectional freeze-drying technology	312:350	unidirectional freeze-drying technology	312:350	In order to capture savings in energy and ambitious environmental targets, biodegradable composite foams of poly(vinyl alcohol) (PVA) supported by cellulose nanofibrils (CNF) were prepared through unidirectional freeze-drying technology.
25062502	2	18	theme	content	394:400	arg1	content					368:374	the content	364:374	the content of CNF, the solid content of the precursor suspension, and the quenching temperature	364:459	Effects of the content of CNF, the solid content of the precursor suspension, and the quenching temperature on the microstructure and properties of the composite foams were investigated by scanning electron microscopy (SEM), compressive testing, X-ray diffraction (XRD) analysis, water uptake, and biodegradation tests.
25062502	3	19	theme	energy	810:815	arg1	absorption					817:826	energy absorption	810:826	energy absorption	810:826	Results show that the incorporation of CNF preferably at a weight ratio of 30 wt % greatly enhanced the mechanical strength and modulus, energy absorption, water resistance, and dimensional stability of the composite foams because of the rigid and semicrystalline nature of CNF as well as regular and compact pore structures.
25062502	3	20	theme	pore	982:985	arg1	structures					987:996	regular and compact pore structures	962:996	regular and compact pore structures	962:996	Results show that the incorporation of CNF preferably at a weight ratio of 30 wt % greatly enhanced the mechanical strength and modulus, energy absorption, water resistance, and dimensional stability of the composite foams because of the rigid and semicrystalline nature of CNF as well as regular and compact pore structures.
25062502	2	21	theme	temperature	449:459	arg1	content					368:374	the content	364:374	the content of CNF, the solid content of the precursor suspension, and the quenching temperature	364:459	Effects of the content of CNF, the solid content of the precursor suspension, and the quenching temperature on the microstructure and properties of the composite foams were investigated by scanning electron microscopy (SEM), compressive testing, X-ray diffraction (XRD) analysis, water uptake, and biodegradation tests.
25062502	5	22	theme	composite	1279:1287	arg1	foams					1289:1293	the biomimetic cellular composite foams	1255:1293	the biomimetic cellular composite foams with controllable morphs and properties by varying the freeze-drying process and composition	1255:1386	Hence, we provided some meaningful information on the biomimetic cellular composite foams with controllable morphs and properties by varying the freeze-drying process and composition.
25062502	0	23	theme	Biodegradable	0:12	arg1	poly					14:17	Biodegradable poly	0:17	Biodegradable poly(vinyl alcohol)	0:32	Biodegradable poly(vinyl alcohol) foams supported by cellulose nanofibrils: processing, structure, and properties.
25062502	0	23	theme	Biodegradable	0:12	arg1	alcohol					25:31	vinyl alcohol	19:31	vinyl alcohol	19:31	Biodegradable poly(vinyl alcohol) foams supported by cellulose nanofibrils: processing, structure, and properties.
25062502	4	24	theme	foams	1198:1202	arg1	biodegradation					1166:1179	biodegradation	1166:1179	biodegradation of the composite foams	1166:1202	Furthermore, the biodegradation tests performed in a simulated aerobic compost environment suggested that the involvement of CNF significantly accelerated the pace of biodegradation of the composite foams.
25062502	0	25	dep	nanofibrils	63:73	arg1	nanofibrils					63:73	cellulose nanofibrils	53:73	cellulose nanofibrils: processing, structure, and properties	53:112	Biodegradable poly(vinyl alcohol) foams supported by cellulose nanofibrils: processing, structure, and properties.
25062502	0	25	dep	nanofibrils	63:73	arg1	structure					88:96	structure	88:96	structure	88:96	Biodegradable poly(vinyl alcohol) foams supported by cellulose nanofibrils: processing, structure, and properties.
25062502	0	25	dep	nanofibrils	63:73	arg1	properties					103:112	properties	103:112	properties	103:112	Biodegradable poly(vinyl alcohol) foams supported by cellulose nanofibrils: processing, structure, and properties.
25062502	0	25	dep	nanofibrils	63:73	arg1	processing					76:85	processing	76:85	processing	76:85	Biodegradable poly(vinyl alcohol) foams supported by cellulose nanofibrils: processing, structure, and properties.
25062502	3	26	theme	preferably	716:725	arg1	incorporation					695:707	the incorporation	691:707	the incorporation of CNF preferably at a weight ratio of 30 wt %	691:754	Results show that the incorporation of CNF preferably at a weight ratio of 30 wt % greatly enhanced the mechanical strength and modulus, energy absorption, water resistance, and dimensional stability of the composite foams because of the rigid and semicrystalline nature of CNF as well as regular and compact pore structures.
25062502	1	27	theme	ambitious	157:165	arg1	targets					181:187	ambitious environmental targets	157:187	ambitious environmental targets	157:187	In order to capture savings in energy and ambitious environmental targets, biodegradable composite foams of poly(vinyl alcohol) (PVA) supported by cellulose nanofibrils (CNF) were prepared through unidirectional freeze-drying technology.
25062502	0	28	theme	vinyl	19:23	arg1	poly					14:17	Biodegradable poly	0:17	Biodegradable poly(vinyl alcohol)	0:32	Biodegradable poly(vinyl alcohol) foams supported by cellulose nanofibrils: processing, structure, and properties.
25062502	0	28	theme	vinyl	19:23	arg1	alcohol					25:31	vinyl alcohol	19:31	vinyl alcohol	19:31	Biodegradable poly(vinyl alcohol) foams supported by cellulose nanofibrils: processing, structure, and properties.
25062502	2	29	theme	composite	505:513	arg1	foams					515:519	the composite foams	501:519	the composite foams	501:519	Effects of the content of CNF, the solid content of the precursor suspension, and the quenching temperature on the microstructure and properties of the composite foams were investigated by scanning electron microscopy (SEM), compressive testing, X-ray diffraction (XRD) analysis, water uptake, and biodegradation tests.
25062502	3	30	theme	CNF	947:949	arg1	nature					937:942	the rigid and semicrystalline nature	907:942	the rigid and semicrystalline nature of CNF	907:949	Results show that the incorporation of CNF preferably at a weight ratio of 30 wt % greatly enhanced the mechanical strength and modulus, energy absorption, water resistance, and dimensional stability of the composite foams because of the rigid and semicrystalline nature of CNF as well as regular and compact pore structures.
25062502	3	31	theme	dimensional	851:861	arg1	stability					863:871	dimensional stability	851:871	dimensional stability	851:871	Results show that the incorporation of CNF preferably at a weight ratio of 30 wt % greatly enhanced the mechanical strength and modulus, energy absorption, water resistance, and dimensional stability of the composite foams because of the rigid and semicrystalline nature of CNF as well as regular and compact pore structures.
25062502	1	32	theme	environmental	167:179	arg1	targets					181:187	ambitious environmental targets	157:187	ambitious environmental targets	157:187	In order to capture savings in energy and ambitious environmental targets, biodegradable composite foams of poly(vinyl alcohol) (PVA) supported by cellulose nanofibrils (CNF) were prepared through unidirectional freeze-drying technology.
25062502	2	33	theme	CNF	379:381	arg1	content					368:374	the content	364:374	the content of CNF, the solid content of the precursor suspension, and the quenching temperature	364:459	Effects of the content of CNF, the solid content of the precursor suspension, and the quenching temperature on the microstructure and properties of the composite foams were investigated by scanning electron microscopy (SEM), compressive testing, X-ray diffraction (XRD) analysis, water uptake, and biodegradation tests.
25062502	5	34	theme	biomimetic	1259:1268	arg1	foams					1289:1293	the biomimetic cellular composite foams	1255:1293	the biomimetic cellular composite foams with controllable morphs and properties by varying the freeze-drying process and composition	1255:1386	Hence, we provided some meaningful information on the biomimetic cellular composite foams with controllable morphs and properties by varying the freeze-drying process and composition.
25062502	5	35	from	information	1240:1250	arg1	foams					1289:1293	the biomimetic cellular composite foams	1255:1293	the biomimetic cellular composite foams with controllable morphs and properties by varying the freeze-drying process and composition	1255:1386	Hence, we provided some meaningful information on the biomimetic cellular composite foams with controllable morphs and properties by varying the freeze-drying process and composition.
25062502	4	36	theme	biodegradation	1016:1029	arg1	tests					1031:1035	the biodegradation tests	1012:1035	the biodegradation tests performed in a simulated aerobic compost environment	1012:1088	Furthermore, the biodegradation tests performed in a simulated aerobic compost environment suggested that the involvement of CNF significantly accelerated the pace of biodegradation of the composite foams.
25062502	2	37	theme	suspension	419:428	arg1	content					394:400	the solid content	384:400	the solid content of the precursor suspension	384:428	Effects of the content of CNF, the solid content of the precursor suspension, and the quenching temperature on the microstructure and properties of the composite foams were investigated by scanning electron microscopy (SEM), compressive testing, X-ray diffraction (XRD) analysis, water uptake, and biodegradation tests.
25062502	2	37	theme	suspension	419:428	arg1	temperature					449:459	the quenching temperature	435:459	the quenching temperature	435:459	Effects of the content of CNF, the solid content of the precursor suspension, and the quenching temperature on the microstructure and properties of the composite foams were investigated by scanning electron microscopy (SEM), compressive testing, X-ray diffraction (XRD) analysis, water uptake, and biodegradation tests.
25062502	2	37	theme	suspension	419:428	arg1	CNF					379:381	CNF	379:381	CNF	379:381	Effects of the content of CNF, the solid content of the precursor suspension, and the quenching temperature on the microstructure and properties of the composite foams were investigated by scanning electron microscopy (SEM), compressive testing, X-ray diffraction (XRD) analysis, water uptake, and biodegradation tests.
25062502	3	38	theme	CNF	712:714	arg1	preferably					716:725	CNF preferably	712:725	CNF preferably	712:725	Results show that the incorporation of CNF preferably at a weight ratio of 30 wt % greatly enhanced the mechanical strength and modulus, energy absorption, water resistance, and dimensional stability of the composite foams because of the rigid and semicrystalline nature of CNF as well as regular and compact pore structures.
25062502	3	39	theme	%	754:754	arg1	ratio					739:743	a weight ratio	730:743	a weight ratio of 30 wt %	730:754	Results show that the incorporation of CNF preferably at a weight ratio of 30 wt % greatly enhanced the mechanical strength and modulus, energy absorption, water resistance, and dimensional stability of the composite foams because of the rigid and semicrystalline nature of CNF as well as regular and compact pore structures.
25062502	0	40	dep	foams	34:38	arg1	supported					40:48	supported	40:48	foams supported by cellulose nanofibrils: processing, structure, and properties	34:112	Biodegradable poly(vinyl alcohol) foams supported by cellulose nanofibrils: processing, structure, and properties.
25062502	2	41	from	Effects	353:359	arg1	microstructure					468:481	the microstructure	464:481	the microstructure	464:481	Effects of the content of CNF, the solid content of the precursor suspension, and the quenching temperature on the microstructure and properties of the composite foams were investigated by scanning electron microscopy (SEM), compressive testing, X-ray diffraction (XRD) analysis, water uptake, and biodegradation tests.
25062502	2	42	theme	content	368:374	arg1	properties					487:496	properties	487:496	properties of the composite foams	487:519	Effects of the content of CNF, the solid content of the precursor suspension, and the quenching temperature on the microstructure and properties of the composite foams were investigated by scanning electron microscopy (SEM), compressive testing, X-ray diffraction (XRD) analysis, water uptake, and biodegradation tests.
25062502	2	42	theme	content	368:374	arg1	Effects					353:359	Effects	353:359	Effects of the content of CNF, the solid content of the precursor suspension, and the quenching temperature on the microstructure	353:481	Effects of the content of CNF, the solid content of the precursor suspension, and the quenching temperature on the microstructure and properties of the composite foams were investigated by scanning electron microscopy (SEM), compressive testing, X-ray diffraction (XRD) analysis, water uptake, and biodegradation tests.
25062502	2	43	theme	precursor	409:417	arg1	suspension					419:428	the precursor suspension	405:428	the precursor suspension	405:428	Effects of the content of CNF, the solid content of the precursor suspension, and the quenching temperature on the microstructure and properties of the composite foams were investigated by scanning electron microscopy (SEM), compressive testing, X-ray diffraction (XRD) analysis, water uptake, and biodegradation tests.
25062502	1	44	theme	cellulose	262:270	arg1	CNF					285:287	CNF	285:287	CNF	285:287	In order to capture savings in energy and ambitious environmental targets, biodegradable composite foams of poly(vinyl alcohol) (PVA) supported by cellulose nanofibrils (CNF) were prepared through unidirectional freeze-drying technology.
25062502	1	44	theme	cellulose	262:270	arg1	nanofibrils					272:282	cellulose nanofibrils	262:282	cellulose nanofibrils (CNF)	262:288	In order to capture savings in energy and ambitious environmental targets, biodegradable composite foams of poly(vinyl alcohol) (PVA) supported by cellulose nanofibrils (CNF) were prepared through unidirectional freeze-drying technology.
25062502	5	45	theme	controllable	1300:1311	arg1	morphs					1313:1318	controllable morphs	1300:1318	controllable morphs	1300:1318	Hence, we provided some meaningful information on the biomimetic cellular composite foams with controllable morphs and properties by varying the freeze-drying process and composition.
25062502	3	46	theme	composite	880:888	arg1	foams					890:894	the composite foams	876:894	the composite foams because of the rigid and semicrystalline nature of CNF	876:949	Results show that the incorporation of CNF preferably at a weight ratio of 30 wt % greatly enhanced the mechanical strength and modulus, energy absorption, water resistance, and dimensional stability of the composite foams because of the rigid and semicrystalline nature of CNF as well as regular and compact pore structures.
25062502	2	47	theme	solid	388:392	arg1	content					394:400	the solid content	384:400	the solid content of the precursor suspension	384:428	Effects of the content of CNF, the solid content of the precursor suspension, and the quenching temperature on the microstructure and properties of the composite foams were investigated by scanning electron microscopy (SEM), compressive testing, X-ray diffraction (XRD) analysis, water uptake, and biodegradation tests.
25062502	4	48	theme	composite	1188:1196	arg1	foams					1198:1202	the composite foams	1184:1202	the composite foams	1184:1202	Furthermore, the biodegradation tests performed in a simulated aerobic compost environment suggested that the involvement of CNF significantly accelerated the pace of biodegradation of the composite foams.
25062502	2	49	theme	water	633:637	arg1	uptake					639:644	water uptake	633:644	water uptake	633:644	Effects of the content of CNF, the solid content of the precursor suspension, and the quenching temperature on the microstructure and properties of the composite foams were investigated by scanning electron microscopy (SEM), compressive testing, X-ray diffraction (XRD) analysis, water uptake, and biodegradation tests.
25062502	5	50	theme	meaningful	1229:1238	arg1	information					1240:1250	some meaningful information	1224:1250	some meaningful information on the biomimetic cellular composite foams with controllable morphs and properties by varying the freeze-drying process and composition	1224:1386	Hence, we provided some meaningful information on the biomimetic cellular composite foams with controllable morphs and properties by varying the freeze-drying process and composition.
25062502	2	51	theme	X-ray	599:603	arg1	diffraction					605:615	X-ray diffraction	599:615	X-ray diffraction	599:615	Effects of the content of CNF, the solid content of the precursor suspension, and the quenching temperature on the microstructure and properties of the composite foams were investigated by scanning electron microscopy (SEM), compressive testing, X-ray diffraction (XRD) analysis, water uptake, and biodegradation tests.
25062502	3	52	theme	foams	890:894	arg1	absorption					817:826	energy absorption	810:826	energy absorption	810:826	Results show that the incorporation of CNF preferably at a weight ratio of 30 wt % greatly enhanced the mechanical strength and modulus, energy absorption, water resistance, and dimensional stability of the composite foams because of the rigid and semicrystalline nature of CNF as well as regular and compact pore structures.
25062502	3	52	theme	foams	890:894	arg1	stability					863:871	dimensional stability	851:871	dimensional stability	851:871	Results show that the incorporation of CNF preferably at a weight ratio of 30 wt % greatly enhanced the mechanical strength and modulus, energy absorption, water resistance, and dimensional stability of the composite foams because of the rigid and semicrystalline nature of CNF as well as regular and compact pore structures.
25062502	3	52	theme	foams	890:894	arg1	strength					788:795	the mechanical strength	773:795	the mechanical strength	773:795	Results show that the incorporation of CNF preferably at a weight ratio of 30 wt % greatly enhanced the mechanical strength and modulus, energy absorption, water resistance, and dimensional stability of the composite foams because of the rigid and semicrystalline nature of CNF as well as regular and compact pore structures.
25062502	3	52	theme	foams	890:894	arg1	structures					987:996	regular and compact pore structures	962:996	regular and compact pore structures	962:996	Results show that the incorporation of CNF preferably at a weight ratio of 30 wt % greatly enhanced the mechanical strength and modulus, energy absorption, water resistance, and dimensional stability of the composite foams because of the rigid and semicrystalline nature of CNF as well as regular and compact pore structures.
25062502	3	52	theme	foams	890:894	arg1	modulus					801:807	modulus	801:807	modulus	801:807	Results show that the incorporation of CNF preferably at a weight ratio of 30 wt % greatly enhanced the mechanical strength and modulus, energy absorption, water resistance, and dimensional stability of the composite foams because of the rigid and semicrystalline nature of CNF as well as regular and compact pore structures.
25062502	3	52	theme	foams	890:894	arg1	resistance					835:844	water resistance	829:844	water resistance	829:844	Results show that the incorporation of CNF preferably at a weight ratio of 30 wt % greatly enhanced the mechanical strength and modulus, energy absorption, water resistance, and dimensional stability of the composite foams because of the rigid and semicrystalline nature of CNF as well as regular and compact pore structures.
25062502	1	53	theme	composite	204:212	arg1	foams					214:218	biodegradable composite foams	190:218	biodegradable composite foams of poly(vinyl alcohol) (PVA) supported by cellulose nanofibrils (CNF)	190:288	In order to capture savings in energy and ambitious environmental targets, biodegradable composite foams of poly(vinyl alcohol) (PVA) supported by cellulose nanofibrils (CNF) were prepared through unidirectional freeze-drying technology.
25062502	2	54	theme	foams	515:519	arg1	properties					487:496	properties	487:496	properties of the composite foams	487:519	Effects of the content of CNF, the solid content of the precursor suspension, and the quenching temperature on the microstructure and properties of the composite foams were investigated by scanning electron microscopy (SEM), compressive testing, X-ray diffraction (XRD) analysis, water uptake, and biodegradation tests.
25062502	2	54	theme	foams	515:519	arg1	Effects					353:359	Effects	353:359	Effects of the content of CNF, the solid content of the precursor suspension, and the quenching temperature on the microstructure	353:481	Effects of the content of CNF, the solid content of the precursor suspension, and the quenching temperature on the microstructure and properties of the composite foams were investigated by scanning electron microscopy (SEM), compressive testing, X-ray diffraction (XRD) analysis, water uptake, and biodegradation tests.
25062502	4	55	theme	compost	1070:1076	arg1	environment					1078:1088	a simulated aerobic compost environment	1050:1088	a simulated aerobic compost environment	1050:1088	Furthermore, the biodegradation tests performed in a simulated aerobic compost environment suggested that the involvement of CNF significantly accelerated the pace of biodegradation of the composite foams.
25062502	4	56	theme	simulated	1052:1060	arg1	environment					1078:1088	a simulated aerobic compost environment	1050:1088	a simulated aerobic compost environment	1050:1088	Furthermore, the biodegradation tests performed in a simulated aerobic compost environment suggested that the involvement of CNF significantly accelerated the pace of biodegradation of the composite foams.
25062502	2	57	from	properties	487:496	arg1	microstructure					468:481	the microstructure	464:481	the microstructure	464:481	Effects of the content of CNF, the solid content of the precursor suspension, and the quenching temperature on the microstructure and properties of the composite foams were investigated by scanning electron microscopy (SEM), compressive testing, X-ray diffraction (XRD) analysis, water uptake, and biodegradation tests.
25062502	0	58	theme	cellulose	53:61	arg1	nanofibrils					63:73	cellulose nanofibrils	53:73	cellulose nanofibrils: processing, structure, and properties	53:112	Biodegradable poly(vinyl alcohol) foams supported by cellulose nanofibrils: processing, structure, and properties.
25062502	0	58	theme	cellulose	53:61	arg1	structure					88:96	structure	88:96	structure	88:96	Biodegradable poly(vinyl alcohol) foams supported by cellulose nanofibrils: processing, structure, and properties.
25062502	0	58	theme	cellulose	53:61	arg1	processing					76:85	processing	76:85	processing	76:85	Biodegradable poly(vinyl alcohol) foams supported by cellulose nanofibrils: processing, structure, and properties.
25062502	1	59	theme	biodegradable	190:202	arg1	foams					214:218	biodegradable composite foams	190:218	biodegradable composite foams of poly(vinyl alcohol) (PVA) supported by cellulose nanofibrils (CNF)	190:288	In order to capture savings in energy and ambitious environmental targets, biodegradable composite foams of poly(vinyl alcohol) (PVA) supported by cellulose nanofibrils (CNF) were prepared through unidirectional freeze-drying technology.
25062502	3	60	theme	nature	937:942	arg1	foams					890:894	the composite foams	876:894	the composite foams because of the rigid and semicrystalline nature of CNF	876:949	Results show that the incorporation of CNF preferably at a weight ratio of 30 wt % greatly enhanced the mechanical strength and modulus, energy absorption, water resistance, and dimensional stability of the composite foams because of the rigid and semicrystalline nature of CNF as well as regular and compact pore structures.
25062502	4	61	theme	aerobic	1062:1068	arg1	environment					1078:1088	a simulated aerobic compost environment	1050:1088	a simulated aerobic compost environment	1050:1088	Furthermore, the biodegradation tests performed in a simulated aerobic compost environment suggested that the involvement of CNF significantly accelerated the pace of biodegradation of the composite foams.
25062502	3	62	theme	mechanical	777:786	arg1	strength					788:795	the mechanical strength	773:795	the mechanical strength	773:795	Results show that the incorporation of CNF preferably at a weight ratio of 30 wt % greatly enhanced the mechanical strength and modulus, energy absorption, water resistance, and dimensional stability of the composite foams because of the rigid and semicrystalline nature of CNF as well as regular and compact pore structures.
25062502	5	63	with	foams	1289:1293	arg1	properties					1324:1333	properties	1324:1333	properties	1324:1333	Hence, we provided some meaningful information on the biomimetic cellular composite foams with controllable morphs and properties by varying the freeze-drying process and composition.
25062502	5	63	with	foams	1289:1293	arg1	morphs					1313:1318	controllable morphs	1300:1318	controllable morphs	1300:1318	Hence, we provided some meaningful information on the biomimetic cellular composite foams with controllable morphs and properties by varying the freeze-drying process and composition.
25062502	2	64	theme	quenching	439:447	arg1	temperature					449:459	the quenching temperature	435:459	the quenching temperature	435:459	Effects of the content of CNF, the solid content of the precursor suspension, and the quenching temperature on the microstructure and properties of the composite foams were investigated by scanning electron microscopy (SEM), compressive testing, X-ray diffraction (XRD) analysis, water uptake, and biodegradation tests.
25062502	2	65	theme	biodegradation	651:664	arg1	tests					666:670	biodegradation tests	651:670	biodegradation tests	651:670	Effects of the content of CNF, the solid content of the precursor suspension, and the quenching temperature on the microstructure and properties of the composite foams were investigated by scanning electron microscopy (SEM), compressive testing, X-ray diffraction (XRD) analysis, water uptake, and biodegradation tests.
25062502	3	66	theme	weight	732:737	arg1	ratio					739:743	a weight ratio	730:743	a weight ratio of 30 wt %	730:754	Results show that the incorporation of CNF preferably at a weight ratio of 30 wt % greatly enhanced the mechanical strength and modulus, energy absorption, water resistance, and dimensional stability of the composite foams because of the rigid and semicrystalline nature of CNF as well as regular and compact pore structures.
25062502	1	67	theme	poly	223:226	arg1	foams					214:218	biodegradable composite foams	190:218	biodegradable composite foams of poly(vinyl alcohol) (PVA) supported by cellulose nanofibrils (CNF)	190:288	In order to capture savings in energy and ambitious environmental targets, biodegradable composite foams of poly(vinyl alcohol) (PVA) supported by cellulose nanofibrils (CNF) were prepared through unidirectional freeze-drying technology.
24559142	0	0	theme	serotype	103:110	arg1	polysaccharides					125:139	the Gram-negative bacterium Shigella flexneri serotype Y. O-Antigen polysaccharides	57:139	the Gram-negative bacterium Shigella flexneri serotype Y. O-Antigen polysaccharides	57:139	Conformational diversity of O-antigen polysaccharides of the Gram-negative bacterium Shigella flexneri serotype Y. O-Antigen polysaccharides constitute the outer protective layer of most Gram-negative bacteria, important for the bacterium's survival and adaption within its host.
24559142	1	1	theme	polysaccharide	364:377	arg1	coat					379:382	the dense polysaccharide coat	354:382	the dense polysaccharide coat	354:382	Although important for many functions, the three-dimensional structure of the dense polysaccharide coat remains to be elucidated.
24559142	0	2	theme	important	211:219	arg1	bacteria					201:208	most Gram-negative bacteria	182:208	most Gram-negative bacteria	182:208	Conformational diversity of O-antigen polysaccharides of the Gram-negative bacterium Shigella flexneri serotype Y. O-Antigen polysaccharides constitute the outer protective layer of most Gram-negative bacteria, important for the bacterium's survival and adaption within its host.
24559142	6	3	theme	chain	1232:1236	arg1	distribution					1245:1256	chain length distribution	1232:1256	chain length distribution	1232:1256	Thus, our computational analysis represents an essential prerequisite for developing reliable coarse-grained models that may help visualizing changes in O-antigen coat morphology upon variations in chain length distribution or chemical composition of the polysaccharide characterizing a certain serotype.
24559142	0	4	theme	flexneri	94:101	arg1	serotype					103:110	the Gram-negative bacterium Shigella flexneri serotype	57:110	the Gram-negative bacterium Shigella flexneri serotype Y. O-Antigen polysaccharides	57:139	Conformational diversity of O-antigen polysaccharides of the Gram-negative bacterium Shigella flexneri serotype Y. O-Antigen polysaccharides constitute the outer protective layer of most Gram-negative bacteria, important for the bacterium's survival and adaption within its host.
24559142	1	5	theme	coat	379:382	arg1	structure					341:349	the three-dimensional structure	319:349	the three-dimensional structure of the dense polysaccharide coat	319:382	Although important for many functions, the three-dimensional structure of the dense polysaccharide coat remains to be elucidated.
24559142	2	6	theme	serotype	529:536	arg1	Y					538:538	serotype Y	529:538	Shigella flexneri serotype Y composed of one up to four tetrasaccharide repeat units	511:594	In this study, we present a systematic numerical investigation of O-antigen polysaccharide chains of Shigella flexneri serotype Y composed of one up to four tetrasaccharide repeat units.
24559142	6	7	theme	chemical	1261:1268	arg1	composition					1270:1280	chemical composition	1261:1280	chemical composition	1261:1280	Thus, our computational analysis represents an essential prerequisite for developing reliable coarse-grained models that may help visualizing changes in O-antigen coat morphology upon variations in chain length distribution or chemical composition of the polysaccharide characterizing a certain serotype.
24559142	0	8	theme	Y.	112:113	arg1	O-Antigen					115:123	Y. O-Antigen	112:123	the Gram-negative bacterium Shigella flexneri serotype Y. O-Antigen polysaccharides	57:139	Conformational diversity of O-antigen polysaccharides of the Gram-negative bacterium Shigella flexneri serotype Y. O-Antigen polysaccharides constitute the outer protective layer of most Gram-negative bacteria, important for the bacterium's survival and adaption within its host.
24559142	5	9	theme	force	989:993	arg1	field					995:999	the force field	985:999	the force field used to model the carbohydrates	985:1031	These can facilitate the formation of metastable compact states, but this conclusion depends sensitively on the force field used to model the carbohydrates.
24559142	6	10	theme	coarse-grained	1128:1141	arg1	models					1143:1148	reliable coarse-grained models	1119:1148	reliable coarse-grained models that may help visualizing changes in O-antigen coat morphology upon variations in chain length distribution or chemical composition of the polysaccharide characterizing a certain serotype	1119:1336	Thus, our computational analysis represents an essential prerequisite for developing reliable coarse-grained models that may help visualizing changes in O-antigen coat morphology upon variations in chain length distribution or chemical composition of the polysaccharide characterizing a certain serotype.
24559142	4	11	dep	extreme	840:846	arg1	hairpin-like					849:860	hairpin-like	849:860	hairpin-like	849:860	It reveals that even for a few repeat units polymer-like flexibility emerges, which is furthermore complemented by extreme, hairpin-like conformations.
24559142	0	12	theme	polysaccharides	125:139	arg1	polysaccharides					38:52	O-antigen polysaccharides	28:52	O-antigen polysaccharides of the Gram-negative bacterium Shigella flexneri serotype Y. O-Antigen polysaccharides	28:139	Conformational diversity of O-antigen polysaccharides of the Gram-negative bacterium Shigella flexneri serotype Y. O-Antigen polysaccharides constitute the outer protective layer of most Gram-negative bacteria, important for the bacterium's survival and adaption within its host.
24559142	6	13	theme	length	1238:1243	arg1	distribution					1245:1256	chain length distribution	1232:1256	chain length distribution	1232:1256	Thus, our computational analysis represents an essential prerequisite for developing reliable coarse-grained models that may help visualizing changes in O-antigen coat morphology upon variations in chain length distribution or chemical composition of the polysaccharide characterizing a certain serotype.
24559142	2	14	theme	O-antigen	476:484	arg1	chains					501:506	O-antigen polysaccharide chains	476:506	O-antigen polysaccharide chains of Shigella flexneri serotype Y composed of one up to four tetrasaccharide repeat units	476:594	In this study, we present a systematic numerical investigation of O-antigen polysaccharide chains of Shigella flexneri serotype Y composed of one up to four tetrasaccharide repeat units.
24559142	2	15	theme	flexneri	520:527	arg1	chains					501:506	O-antigen polysaccharide chains	476:506	O-antigen polysaccharide chains of Shigella flexneri serotype Y composed of one up to four tetrasaccharide repeat units	476:594	In this study, we present a systematic numerical investigation of O-antigen polysaccharide chains of Shigella flexneri serotype Y composed of one up to four tetrasaccharide repeat units.
24559142	0	16	dep	serotype	103:110	arg1	O-Antigen					115:123	Y. O-Antigen	112:123	the Gram-negative bacterium Shigella flexneri serotype Y. O-Antigen polysaccharides	57:139	Conformational diversity of O-antigen polysaccharides of the Gram-negative bacterium Shigella flexneri serotype Y. O-Antigen polysaccharides constitute the outer protective layer of most Gram-negative bacteria, important for the bacterium's survival and adaption within its host.
24559142	5	17	theme	metastable	915:924	arg1	states					934:939	metastable compact states	915:939	metastable compact states	915:939	These can facilitate the formation of metastable compact states, but this conclusion depends sensitively on the force field used to model the carbohydrates.
24559142	0	18	theme	Conformational	0:13	arg1	diversity					15:23	Conformational diversity	0:23	Conformational diversity of O-antigen polysaccharides of the Gram-negative bacterium Shigella flexneri serotype Y. O-Antigen polysaccharides	0:139	Conformational diversity of O-antigen polysaccharides of the Gram-negative bacterium Shigella flexneri serotype Y. O-Antigen polysaccharides constitute the outer protective layer of most Gram-negative bacteria, important for the bacterium's survival and adaption within its host.
24559142	1	19	theme	many	303:306	arg1	functions					308:316	many functions	303:316	many functions	303:316	Although important for many functions, the three-dimensional structure of the dense polysaccharide coat remains to be elucidated.
24559142	4	20	theme	repeat	756:761	arg1	units					763:767	a few repeat units	750:767	a few repeat units	750:767	It reveals that even for a few repeat units polymer-like flexibility emerges, which is furthermore complemented by extreme, hairpin-like conformations.
24559142	6	21	from	variations	1218:1227	arg1	distribution					1245:1256	chain length distribution	1232:1256	chain length distribution	1232:1256	Thus, our computational analysis represents an essential prerequisite for developing reliable coarse-grained models that may help visualizing changes in O-antigen coat morphology upon variations in chain length distribution or chemical composition of the polysaccharide characterizing a certain serotype.
24559142	6	21	from	variations	1218:1227	arg1	composition					1270:1280	chemical composition	1261:1280	chemical composition	1261:1280	Thus, our computational analysis represents an essential prerequisite for developing reliable coarse-grained models that may help visualizing changes in O-antigen coat morphology upon variations in chain length distribution or chemical composition of the polysaccharide characterizing a certain serotype.
24559142	5	22	theme	compact	926:932	arg1	states					934:939	metastable compact states	915:939	metastable compact states	915:939	These can facilitate the formation of metastable compact states, but this conclusion depends sensitively on the force field used to model the carbohydrates.
24559142	0	23	theme	O-antigen	28:36	arg1	polysaccharides					38:52	O-antigen polysaccharides	28:52	O-antigen polysaccharides of the Gram-negative bacterium Shigella flexneri serotype Y. O-Antigen polysaccharides	28:139	Conformational diversity of O-antigen polysaccharides of the Gram-negative bacterium Shigella flexneri serotype Y. O-Antigen polysaccharides constitute the outer protective layer of most Gram-negative bacteria, important for the bacterium's survival and adaption within its host.
24559142	4	24	theme	extreme	840:846	arg1	conformations					862:874	extreme, hairpin-like conformations	840:874	extreme, hairpin-like conformations	840:874	It reveals that even for a few repeat units polymer-like flexibility emerges, which is furthermore complemented by extreme, hairpin-like conformations.
24559142	0	25	theme	outer	156:160	arg1	layer					173:177	the outer protective layer	152:177	the outer protective layer of most Gram-negative bacteria, important for the bacterium's survival and adaption within its host	152:277	Conformational diversity of O-antigen polysaccharides of the Gram-negative bacterium Shigella flexneri serotype Y. O-Antigen polysaccharides constitute the outer protective layer of most Gram-negative bacteria, important for the bacterium's survival and adaption within its host.
24559142	2	26	theme	systematic	438:447	arg1	investigation					459:471	a systematic numerical investigation	436:471	a systematic numerical investigation of O-antigen polysaccharide chains of Shigella flexneri serotype Y composed of one up to four tetrasaccharide repeat units	436:594	In this study, we present a systematic numerical investigation of O-antigen polysaccharide chains of Shigella flexneri serotype Y composed of one up to four tetrasaccharide repeat units.
24559142	2	27	theme	numerical	449:457	arg1	investigation					459:471	a systematic numerical investigation	436:471	a systematic numerical investigation of O-antigen polysaccharide chains of Shigella flexneri serotype Y composed of one up to four tetrasaccharide repeat units	436:594	In this study, we present a systematic numerical investigation of O-antigen polysaccharide chains of Shigella flexneri serotype Y composed of one up to four tetrasaccharide repeat units.
24559142	2	28	theme	repeat	583:588	arg1	units					590:594	one up to four tetrasaccharide repeat units	552:594	one up to four tetrasaccharide repeat units	552:594	In this study, we present a systematic numerical investigation of O-antigen polysaccharide chains of Shigella flexneri serotype Y composed of one up to four tetrasaccharide repeat units.
24559142	4	29	theme	polymer-like	769:780	arg1	flexibility					782:792	polymer-like flexibility	769:792	polymer-like flexibility	769:792	It reveals that even for a few repeat units polymer-like flexibility emerges, which is furthermore complemented by extreme, hairpin-like conformations.
24559142	2	30	theme	chains	501:506	arg1	investigation					459:471	a systematic numerical investigation	436:471	a systematic numerical investigation of O-antigen polysaccharide chains of Shigella flexneri serotype Y composed of one up to four tetrasaccharide repeat units	436:594	In this study, we present a systematic numerical investigation of O-antigen polysaccharide chains of Shigella flexneri serotype Y composed of one up to four tetrasaccharide repeat units.
24559142	2	31	theme	Shigella	511:518	arg1	flexneri					520:527	Shigella flexneri serotype Y composed of one up to four tetrasaccharide repeat units	511:594	Shigella flexneri serotype Y composed of one up to four tetrasaccharide repeat units	511:594	In this study, we present a systematic numerical investigation of O-antigen polysaccharide chains of Shigella flexneri serotype Y composed of one up to four tetrasaccharide repeat units.
24559142	2	32	theme	tetrasaccharide	567:581	arg1	units					590:594	one up to four tetrasaccharide repeat units	552:594	one up to four tetrasaccharide repeat units	552:594	In this study, we present a systematic numerical investigation of O-antigen polysaccharide chains of Shigella flexneri serotype Y composed of one up to four tetrasaccharide repeat units.
24559142	3	33	theme	atomistic	623:631	arg1	levels					652:657	atomistic and coarse-grained levels	623:657	atomistic and coarse-grained levels of description	623:672	To bridge the gap between atomistic and coarse-grained levels of description, we employ a genuine multiscale modeling approach.
24559142	6	34	theme	reliable	1119:1126	arg1	models					1143:1148	reliable coarse-grained models	1119:1148	reliable coarse-grained models that may help visualizing changes in O-antigen coat morphology upon variations in chain length distribution or chemical composition of the polysaccharide characterizing a certain serotype	1119:1336	Thus, our computational analysis represents an essential prerequisite for developing reliable coarse-grained models that may help visualizing changes in O-antigen coat morphology upon variations in chain length distribution or chemical composition of the polysaccharide characterizing a certain serotype.
24559142	0	35	theme	polysaccharides	38:52	arg1	diversity					15:23	Conformational diversity	0:23	Conformational diversity of O-antigen polysaccharides of the Gram-negative bacterium Shigella flexneri serotype Y. O-Antigen polysaccharides	0:139	Conformational diversity of O-antigen polysaccharides of the Gram-negative bacterium Shigella flexneri serotype Y. O-Antigen polysaccharides constitute the outer protective layer of most Gram-negative bacteria, important for the bacterium's survival and adaption within its host.
24559142	1	36	theme	three-dimensional	323:339	arg1	structure					341:349	the three-dimensional structure	319:349	the three-dimensional structure of the dense polysaccharide coat	319:382	Although important for many functions, the three-dimensional structure of the dense polysaccharide coat remains to be elucidated.
24559142	0	37	theme	protective	162:171	arg1	layer					173:177	the outer protective layer	152:177	the outer protective layer of most Gram-negative bacteria, important for the bacterium's survival and adaption within its host	152:277	Conformational diversity of O-antigen polysaccharides of the Gram-negative bacterium Shigella flexneri serotype Y. O-Antigen polysaccharides constitute the outer protective layer of most Gram-negative bacteria, important for the bacterium's survival and adaption within its host.
24559142	6	38	theme	certain	1321:1327	arg1	serotype					1329:1336	a certain serotype	1319:1336	a certain serotype	1319:1336	Thus, our computational analysis represents an essential prerequisite for developing reliable coarse-grained models that may help visualizing changes in O-antigen coat morphology upon variations in chain length distribution or chemical composition of the polysaccharide characterizing a certain serotype.
24559142	6	39	theme	essential	1081:1089	arg1	prerequisite					1091:1102	an essential prerequisite	1078:1102	an essential prerequisite for developing reliable coarse-grained models that may help visualizing changes in O-antigen coat morphology upon variations in chain length distribution or chemical composition of the polysaccharide characterizing a certain serotype	1078:1336	Thus, our computational analysis represents an essential prerequisite for developing reliable coarse-grained models that may help visualizing changes in O-antigen coat morphology upon variations in chain length distribution or chemical composition of the polysaccharide characterizing a certain serotype.
24559142	0	40	theme	Gram-negative	61:73	arg1	bacterium					75:83	the Gram-negative bacterium	57:83	the Gram-negative bacterium Shigella flexneri serotype Y. O-Antigen polysaccharides	57:139	Conformational diversity of O-antigen polysaccharides of the Gram-negative bacterium Shigella flexneri serotype Y. O-Antigen polysaccharides constitute the outer protective layer of most Gram-negative bacteria, important for the bacterium's survival and adaption within its host.
24559142	0	41	theme	most	182:185	arg1	bacteria					201:208	most Gram-negative bacteria	182:208	most Gram-negative bacteria	182:208	Conformational diversity of O-antigen polysaccharides of the Gram-negative bacterium Shigella flexneri serotype Y. O-Antigen polysaccharides constitute the outer protective layer of most Gram-negative bacteria, important for the bacterium's survival and adaption within its host.
24559142	2	42	attach	present	428:434	arg1	study					418:422	this study	413:422	this study	413:422	In this study, we present a systematic numerical investigation of O-antigen polysaccharide chains of Shigella flexneri serotype Y composed of one up to four tetrasaccharide repeat units.
24559142	2	42	attach	present	428:434	arg2	we					425:426	we	425:426	we	425:426	In this study, we present a systematic numerical investigation of O-antigen polysaccharide chains of Shigella flexneri serotype Y composed of one up to four tetrasaccharide repeat units.
24559142	2	43	theme	polysaccharide	486:499	arg1	chains					501:506	O-antigen polysaccharide chains	476:506	O-antigen polysaccharide chains of Shigella flexneri serotype Y composed of one up to four tetrasaccharide repeat units	476:594	In this study, we present a systematic numerical investigation of O-antigen polysaccharide chains of Shigella flexneri serotype Y composed of one up to four tetrasaccharide repeat units.
24559142	2	44	dep	flexneri	520:527	arg1	Y					538:538	serotype Y	529:538	Shigella flexneri serotype Y composed of one up to four tetrasaccharide repeat units	511:594	In this study, we present a systematic numerical investigation of O-antigen polysaccharide chains of Shigella flexneri serotype Y composed of one up to four tetrasaccharide repeat units.
24559142	3	45	theme	modeling	706:713	arg1	approach					715:722	a genuine multiscale modeling approach	685:722	a genuine multiscale modeling approach	685:722	To bridge the gap between atomistic and coarse-grained levels of description, we employ a genuine multiscale modeling approach.
24559142	6	46	theme	coat	1197:1200	arg1	morphology					1202:1211	O-antigen coat morphology	1187:1211	O-antigen coat morphology	1187:1211	Thus, our computational analysis represents an essential prerequisite for developing reliable coarse-grained models that may help visualizing changes in O-antigen coat morphology upon variations in chain length distribution or chemical composition of the polysaccharide characterizing a certain serotype.
24559142	0	47	theme	bacteria	201:208	arg1	layer					173:177	the outer protective layer	152:177	the outer protective layer of most Gram-negative bacteria, important for the bacterium's survival and adaption within its host	152:277	Conformational diversity of O-antigen polysaccharides of the Gram-negative bacterium Shigella flexneri serotype Y. O-Antigen polysaccharides constitute the outer protective layer of most Gram-negative bacteria, important for the bacterium's survival and adaption within its host.
24559142	6	48	theme	polysaccharide	1289:1302	arg1	distribution					1245:1256	chain length distribution	1232:1256	chain length distribution	1232:1256	Thus, our computational analysis represents an essential prerequisite for developing reliable coarse-grained models that may help visualizing changes in O-antigen coat morphology upon variations in chain length distribution or chemical composition of the polysaccharide characterizing a certain serotype.
24559142	6	48	theme	polysaccharide	1289:1302	arg1	composition					1270:1280	chemical composition	1261:1280	chemical composition	1261:1280	Thus, our computational analysis represents an essential prerequisite for developing reliable coarse-grained models that may help visualizing changes in O-antigen coat morphology upon variations in chain length distribution or chemical composition of the polysaccharide characterizing a certain serotype.
24559142	4	49	theme	few	752:754	arg1	units					763:767	a few repeat units	750:767	a few repeat units	750:767	It reveals that even for a few repeat units polymer-like flexibility emerges, which is furthermore complemented by extreme, hairpin-like conformations.
24559142	0	50	theme	Gram-negative	187:199	arg1	bacteria					201:208	most Gram-negative bacteria	182:208	most Gram-negative bacteria	182:208	Conformational diversity of O-antigen polysaccharides of the Gram-negative bacterium Shigella flexneri serotype Y. O-Antigen polysaccharides constitute the outer protective layer of most Gram-negative bacteria, important for the bacterium's survival and adaption within its host.
24559142	3	51	theme	genuine	687:693	arg1	approach					715:722	a genuine multiscale modeling approach	685:722	a genuine multiscale modeling approach	685:722	To bridge the gap between atomistic and coarse-grained levels of description, we employ a genuine multiscale modeling approach.
24559142	3	52	theme	description	662:672	arg1	levels					652:657	atomistic and coarse-grained levels	623:657	atomistic and coarse-grained levels of description	623:672	To bridge the gap between atomistic and coarse-grained levels of description, we employ a genuine multiscale modeling approach.
24559142	6	53	theme	O-antigen	1187:1195	arg1	morphology					1202:1211	O-antigen coat morphology	1187:1211	O-antigen coat morphology	1187:1211	Thus, our computational analysis represents an essential prerequisite for developing reliable coarse-grained models that may help visualizing changes in O-antigen coat morphology upon variations in chain length distribution or chemical composition of the polysaccharide characterizing a certain serotype.
24559142	0	54	theme	Shigella	85:92	arg1	flexneri					94:101	the Gram-negative bacterium Shigella flexneri	57:101	the Gram-negative bacterium Shigella flexneri serotype Y. O-Antigen polysaccharides	57:139	Conformational diversity of O-antigen polysaccharides of the Gram-negative bacterium Shigella flexneri serotype Y. O-Antigen polysaccharides constitute the outer protective layer of most Gram-negative bacteria, important for the bacterium's survival and adaption within its host.
24559142	3	55	theme	multiscale	695:704	arg1	approach					715:722	a genuine multiscale modeling approach	685:722	a genuine multiscale modeling approach	685:722	To bridge the gap between atomistic and coarse-grained levels of description, we employ a genuine multiscale modeling approach.
24559142	3	56	theme	coarse-grained	637:650	arg1	levels					652:657	atomistic and coarse-grained levels	623:657	atomistic and coarse-grained levels of description	623:672	To bridge the gap between atomistic and coarse-grained levels of description, we employ a genuine multiscale modeling approach.
24559142	5	57	theme	states	934:939	arg1	formation					902:910	the formation	898:910	the formation of metastable compact states	898:939	These can facilitate the formation of metastable compact states, but this conclusion depends sensitively on the force field used to model the carbohydrates.
24559142	0	58	theme	bacterium	75:83	arg1	flexneri					94:101	the Gram-negative bacterium Shigella flexneri	57:101	the Gram-negative bacterium Shigella flexneri serotype Y. O-Antigen polysaccharides	57:139	Conformational diversity of O-antigen polysaccharides of the Gram-negative bacterium Shigella flexneri serotype Y. O-Antigen polysaccharides constitute the outer protective layer of most Gram-negative bacteria, important for the bacterium's survival and adaption within its host.
24559142	1	59	theme	dense	358:362	arg1	coat					379:382	the dense polysaccharide coat	354:382	the dense polysaccharide coat	354:382	Although important for many functions, the three-dimensional structure of the dense polysaccharide coat remains to be elucidated.
24559142	6	60	theme	computational	1044:1056	arg1	analysis					1058:1065	our computational analysis	1040:1065	our computational analysis	1040:1065	Thus, our computational analysis represents an essential prerequisite for developing reliable coarse-grained models that may help visualizing changes in O-antigen coat morphology upon variations in chain length distribution or chemical composition of the polysaccharide characterizing a certain serotype.
24559142	6	61	from	changes	1176:1182	arg1	morphology					1202:1211	O-antigen coat morphology	1187:1211	O-antigen coat morphology	1187:1211	Thus, our computational analysis represents an essential prerequisite for developing reliable coarse-grained models that may help visualizing changes in O-antigen coat morphology upon variations in chain length distribution or chemical composition of the polysaccharide characterizing a certain serotype.
24559142	2	62	dep	four	562:565	arg1	up					556:557	up	556:557	up	556:557	In this study, we present a systematic numerical investigation of O-antigen polysaccharide chains of Shigella flexneri serotype Y composed of one up to four tetrasaccharide repeat units.
24559142	2	62	dep	four	562:565	arg1	to					559:560	to	559:560	to	559:560	In this study, we present a systematic numerical investigation of O-antigen polysaccharide chains of Shigella flexneri serotype Y composed of one up to four tetrasaccharide repeat units.
24548747	5	0	theme	Cyclin	660:665	arg1	B1					667:668	Cyclin B1	660:668	Cyclin B1	660:668	Moreover, AVAP I dramatically reduced the mRNA levels of CDK6 and Cyclin E1 but significantly increased Cyclin B1, CDK1 and Cyclin F. Interestingly, AVAP I remained able to induce cell proliferation in a low serum concentration medium.
24548747	5	1	theme	serum	764:768	arg1	concentration					770:782	a low serum concentration	758:782	a low serum concentration medium	758:789	Moreover, AVAP I dramatically reduced the mRNA levels of CDK6 and Cyclin E1 but significantly increased Cyclin B1, CDK1 and Cyclin F. Interestingly, AVAP I remained able to induce cell proliferation in a low serum concentration medium.
24548747	4	2	theme	AVAP	502:505	arg1	I					507:507	AVAP I	502:507	AVAP I	502:507	The oligosaccharide of AVAP I (Oli-AVAP I) exhibited the same growth effects, but rhamnose, the primary monosaccharide of AVAP I and Oli-AVAP I, did not exhibit this activity.
24548747	5	3	dep	B1	667:668	arg1	F.					687:688	F.	687:688	F.	687:688	Moreover, AVAP I dramatically reduced the mRNA levels of CDK6 and Cyclin E1 but significantly increased Cyclin B1, CDK1 and Cyclin F. Interestingly, AVAP I remained able to induce cell proliferation in a low serum concentration medium.
24548747	4	4	theme	growth	442:447	arg1	effects					449:455	the same growth effects	433:455	the same growth effects	433:455	The oligosaccharide of AVAP I (Oli-AVAP I) exhibited the same growth effects, but rhamnose, the primary monosaccharide of AVAP I and Oli-AVAP I, did not exhibit this activity.
24548747	7	5	theme	mammalian	999:1007	arg1	cells					1009:1013	mammalian cells	999:1013	mammalian cells	999:1013	AVAP I may be useful as a serum supplement for stimulating the proliferation of mammalian cells.
24548747	5	6	theme	concentration	770:782	arg1	medium					784:789	a low serum concentration medium	758:789	a low serum concentration medium	758:789	Moreover, AVAP I dramatically reduced the mRNA levels of CDK6 and Cyclin E1 but significantly increased Cyclin B1, CDK1 and Cyclin F. Interestingly, AVAP I remained able to induce cell proliferation in a low serum concentration medium.
24548747	0	7	theme	cell	78:81	arg1	proliferation					83:95	HepG2 cell proliferation	72:95	HepG2 cell proliferation	72:95	Effects of polysaccharides from abalone (Haliotis discus hannai Ino) on HepG2 cell proliferation.
24548747	0	8	from	Effects	0:6	arg1	proliferation					83:95	HepG2 cell proliferation	72:95	HepG2 cell proliferation	72:95	Effects of polysaccharides from abalone (Haliotis discus hannai Ino) on HepG2 cell proliferation.
24548747	0	8	from	Effects	0:6	arg1	Ino					64:66	Haliotis discus hannai Ino	41:66	Haliotis discus hannai Ino	41:66	Effects of polysaccharides from abalone (Haliotis discus hannai Ino) on HepG2 cell proliferation.
24548747	0	8	from	Effects	0:6	arg1	abalone					32:38	abalone	32:38	abalone (Haliotis discus hannai Ino)	32:67	Effects of polysaccharides from abalone (Haliotis discus hannai Ino) on HepG2 cell proliferation.
24548747	5	9	theme	low	760:762	arg1	concentration					770:782	a low serum concentration	758:782	a low serum concentration medium	758:789	Moreover, AVAP I dramatically reduced the mRNA levels of CDK6 and Cyclin E1 but significantly increased Cyclin B1, CDK1 and Cyclin F. Interestingly, AVAP I remained able to induce cell proliferation in a low serum concentration medium.
24548747	2	10	from	effects	261:267	arg1	proliferation					283:295	HepG2 cell proliferation	272:295	HepG2 cell proliferation	272:295	The polysaccharides' compositions were analysed, and their effects on HepG2 cell proliferation were assessed.
24548747	0	11	theme	HepG2	72:76	arg1	proliferation					83:95	HepG2 cell proliferation	72:95	HepG2 cell proliferation	72:95	Effects of polysaccharides from abalone (Haliotis discus hannai Ino) on HepG2 cell proliferation.
24548747	4	12	theme	Oli-AVAP	411:418	arg1	I					420:420	Oli-AVAP I	411:420	Oli-AVAP I	411:420	The oligosaccharide of AVAP I (Oli-AVAP I) exhibited the same growth effects, but rhamnose, the primary monosaccharide of AVAP I and Oli-AVAP I, did not exhibit this activity.
24548747	4	12	theme	Oli-AVAP	411:418	arg1	I					408:408	AVAP I	403:408	AVAP I (Oli-AVAP I)	403:421	The oligosaccharide of AVAP I (Oli-AVAP I) exhibited the same growth effects, but rhamnose, the primary monosaccharide of AVAP I and Oli-AVAP I, did not exhibit this activity.
24548747	5	13	theme	AVAP	566:569	arg1	I					571:571	AVAP I	566:571	AVAP I	566:571	Moreover, AVAP I dramatically reduced the mRNA levels of CDK6 and Cyclin E1 but significantly increased Cyclin B1, CDK1 and Cyclin F. Interestingly, AVAP I remained able to induce cell proliferation in a low serum concentration medium.
24548747	4	14	theme	I	507:507	arg1	rhamnose					462:469	rhamnose	462:469	rhamnose	462:469	The oligosaccharide of AVAP I (Oli-AVAP I) exhibited the same growth effects, but rhamnose, the primary monosaccharide of AVAP I and Oli-AVAP I, did not exhibit this activity.
24548747	4	14	theme	I	507:507	arg1	monosaccharide					484:497	the primary monosaccharide	472:497	the primary monosaccharide of AVAP I and Oli-AVAP I	472:522	The oligosaccharide of AVAP I (Oli-AVAP I) exhibited the same growth effects, but rhamnose, the primary monosaccharide of AVAP I and Oli-AVAP I, did not exhibit this activity.
24548747	5	15	theme	E1	629:630	arg1	levels					603:608	the mRNA levels	594:608	the mRNA levels of CDK6 and Cyclin E1	594:630	Moreover, AVAP I dramatically reduced the mRNA levels of CDK6 and Cyclin E1 but significantly increased Cyclin B1, CDK1 and Cyclin F. Interestingly, AVAP I remained able to induce cell proliferation in a low serum concentration medium.
24548747	6	16	theme	gene	862:865	arg1	expression					867:876	gene expression	862:876	gene expression	862:876	AVAP I could therefore promote HepG2 cell proliferation by regulating gene expression and accelerating the cell cycle process.
24548747	4	17	theme	Oli-AVAP	513:520	arg1	I					522:522	Oli-AVAP I	513:522	Oli-AVAP I	513:522	The oligosaccharide of AVAP I (Oli-AVAP I) exhibited the same growth effects, but rhamnose, the primary monosaccharide of AVAP I and Oli-AVAP I, did not exhibit this activity.
24548747	1	18	theme	AVAP	138:141	arg1	II					143:144	AVAP II	138:144	AVAP II	138:144	Three polysaccharides, AAP, AVAP I, and AVAP II, were isolated from abalone Haliotis discus hannai Ino.
24548747	1	18	theme	AVAP	138:141	arg1	polysaccharides					104:118	Three polysaccharides	98:118	Three polysaccharides	98:118	Three polysaccharides, AAP, AVAP I, and AVAP II, were isolated from abalone Haliotis discus hannai Ino.
24548747	3	19	theme	greater	325:331	arg1	effect					352:357	a greater growth-stimulatory effect	323:357	a greater growth-stimulatory effect	323:357	AVAP I had a greater growth-stimulatory effect than AAP or AVAP II.
24548747	0	20	from	abalone	32:38	arg1	polysaccharides					11:25	polysaccharides	11:25	polysaccharides from abalone (Haliotis discus hannai Ino)	11:67	Effects of polysaccharides from abalone (Haliotis discus hannai Ino) on HepG2 cell proliferation.
24548747	0	20	from	abalone	32:38	arg1	Effects					0:6	Effects	0:6	Effects of polysaccharides from abalone (Haliotis discus hannai Ino) on HepG2 cell proliferation	0:95	Effects of polysaccharides from abalone (Haliotis discus hannai Ino) on HepG2 cell proliferation.
24548747	7	21	theme	serum	945:949	arg1	supplement					951:960	a serum supplement	943:960	a serum supplement for stimulating the proliferation of mammalian cells	943:1013	AVAP I may be useful as a serum supplement for stimulating the proliferation of mammalian cells.
24548747	3	22	contain	had	319:321	arg1	AVAP					312:315	AVAP I	312:317	AVAP I	312:317	AVAP I had a greater growth-stimulatory effect than AAP or AVAP II.
24548747	3	22	contain	had	319:321	arg2	effect					352:357	a greater growth-stimulatory effect	323:357	a greater growth-stimulatory effect	323:357	AVAP I had a greater growth-stimulatory effect than AAP or AVAP II.
24548747	3	23	theme	growth-stimulatory	333:350	arg1	effect					352:357	a greater growth-stimulatory effect	323:357	a greater growth-stimulatory effect	323:357	AVAP I had a greater growth-stimulatory effect than AAP or AVAP II.
24548747	8	24	theme	abalone	1075:1081	arg1	waste					1122:1126	waste	1122:1126	waste	1122:1126	Our results offer a comprehensive method for utilising the abalone viscera, which is usually discarded as waste.
24548747	8	24	theme	abalone	1075:1081	arg1	viscera					1083:1089	the abalone viscera	1071:1089	the abalone viscera	1071:1089	Our results offer a comprehensive method for utilising the abalone viscera, which is usually discarded as waste.
24548747	8	25	theme	comprehensive	1036:1048	arg1	method					1050:1055	a comprehensive method	1034:1055	a comprehensive method for utilising the abalone viscera, which is usually discarded as waste	1034:1126	Our results offer a comprehensive method for utilising the abalone viscera, which is usually discarded as waste.
24548747	5	26	theme	mRNA	598:601	arg1	levels					603:608	the mRNA levels	594:608	the mRNA levels of CDK6 and Cyclin E1	594:630	Moreover, AVAP I dramatically reduced the mRNA levels of CDK6 and Cyclin E1 but significantly increased Cyclin B1, CDK1 and Cyclin F. Interestingly, AVAP I remained able to induce cell proliferation in a low serum concentration medium.
24548747	0	27	theme	polysaccharides	11:25	arg1	Effects					0:6	Effects	0:6	Effects of polysaccharides from abalone (Haliotis discus hannai Ino) on HepG2 cell proliferation	0:95	Effects of polysaccharides from abalone (Haliotis discus hannai Ino) on HepG2 cell proliferation.
24548747	4	28	theme	I	522:522	arg1	rhamnose					462:469	rhamnose	462:469	rhamnose	462:469	The oligosaccharide of AVAP I (Oli-AVAP I) exhibited the same growth effects, but rhamnose, the primary monosaccharide of AVAP I and Oli-AVAP I, did not exhibit this activity.
24548747	4	28	theme	I	522:522	arg1	monosaccharide					484:497	the primary monosaccharide	472:497	the primary monosaccharide of AVAP I and Oli-AVAP I	472:522	The oligosaccharide of AVAP I (Oli-AVAP I) exhibited the same growth effects, but rhamnose, the primary monosaccharide of AVAP I and Oli-AVAP I, did not exhibit this activity.
24548747	4	29	theme	primary	476:482	arg1	rhamnose					462:469	rhamnose	462:469	rhamnose	462:469	The oligosaccharide of AVAP I (Oli-AVAP I) exhibited the same growth effects, but rhamnose, the primary monosaccharide of AVAP I and Oli-AVAP I, did not exhibit this activity.
24548747	4	29	theme	primary	476:482	arg1	monosaccharide					484:497	the primary monosaccharide	472:497	the primary monosaccharide of AVAP I and Oli-AVAP I	472:522	The oligosaccharide of AVAP I (Oli-AVAP I) exhibited the same growth effects, but rhamnose, the primary monosaccharide of AVAP I and Oli-AVAP I, did not exhibit this activity.
24548747	6	30	theme	cell	829:832	arg1	proliferation					834:846	HepG2 cell proliferation	823:846	HepG2 cell proliferation	823:846	AVAP I could therefore promote HepG2 cell proliferation by regulating gene expression and accelerating the cell cycle process.
24548747	7	31	theme	cells	1009:1013	arg1	proliferation					982:994	the proliferation	978:994	the proliferation of mammalian cells	978:1013	AVAP I may be useful as a serum supplement for stimulating the proliferation of mammalian cells.
24548747	6	32	theme	HepG2	823:827	arg1	proliferation					834:846	HepG2 cell proliferation	823:846	HepG2 cell proliferation	823:846	AVAP I could therefore promote HepG2 cell proliferation by regulating gene expression and accelerating the cell cycle process.
24548747	5	33	theme	CDK6	613:616	arg1	levels					603:608	the mRNA levels	594:608	the mRNA levels of CDK6 and Cyclin E1	594:630	Moreover, AVAP I dramatically reduced the mRNA levels of CDK6 and Cyclin E1 but significantly increased Cyclin B1, CDK1 and Cyclin F. Interestingly, AVAP I remained able to induce cell proliferation in a low serum concentration medium.
24548747	4	34	theme	I	408:408	arg1	oligosaccharide					384:398	The oligosaccharide	380:398	The oligosaccharide of AVAP I (Oli-AVAP I)	380:421	The oligosaccharide of AVAP I (Oli-AVAP I) exhibited the same growth effects, but rhamnose, the primary monosaccharide of AVAP I and Oli-AVAP I, did not exhibit this activity.
24548747	6	35	theme	cell	899:902	arg1	process					910:916	the cell cycle process	895:916	the cell cycle process	895:916	AVAP I could therefore promote HepG2 cell proliferation by regulating gene expression and accelerating the cell cycle process.
24548747	1	36	theme	abalone	166:172	arg1	Ino					197:199	abalone Haliotis discus hannai Ino	166:199	abalone Haliotis discus hannai Ino	166:199	Three polysaccharides, AAP, AVAP I, and AVAP II, were isolated from abalone Haliotis discus hannai Ino.
24548747	0	37	theme	discus	50:55	arg1	Ino					64:66	Haliotis discus hannai Ino	41:66	Haliotis discus hannai Ino	41:66	Effects of polysaccharides from abalone (Haliotis discus hannai Ino) on HepG2 cell proliferation.
24548747	0	37	theme	discus	50:55	arg1	abalone					32:38	abalone	32:38	abalone (Haliotis discus hannai Ino)	32:67	Effects of polysaccharides from abalone (Haliotis discus hannai Ino) on HepG2 cell proliferation.
24548747	4	38	theme	AVAP	403:406	arg1	I					420:420	Oli-AVAP I	411:420	Oli-AVAP I	411:420	The oligosaccharide of AVAP I (Oli-AVAP I) exhibited the same growth effects, but rhamnose, the primary monosaccharide of AVAP I and Oli-AVAP I, did not exhibit this activity.
24548747	4	38	theme	AVAP	403:406	arg1	I					408:408	AVAP I	403:408	AVAP I (Oli-AVAP I)	403:421	The oligosaccharide of AVAP I (Oli-AVAP I) exhibited the same growth effects, but rhamnose, the primary monosaccharide of AVAP I and Oli-AVAP I, did not exhibit this activity.
24548747	0	39	theme	Haliotis	41:48	arg1	Ino					64:66	Haliotis discus hannai Ino	41:66	Haliotis discus hannai Ino	41:66	Effects of polysaccharides from abalone (Haliotis discus hannai Ino) on HepG2 cell proliferation.
24548747	0	39	theme	Haliotis	41:48	arg1	abalone					32:38	abalone	32:38	abalone (Haliotis discus hannai Ino)	32:67	Effects of polysaccharides from abalone (Haliotis discus hannai Ino) on HepG2 cell proliferation.
24548747	1	40	attach	isolated	152:159	arg2	I					131:131	AVAP I	126:131	AVAP I	126:131	Three polysaccharides, AAP, AVAP I, and AVAP II, were isolated from abalone Haliotis discus hannai Ino.
24548747	1	40	attach	isolated	152:159	arg1	Ino					197:199	abalone Haliotis discus hannai Ino	166:199	abalone Haliotis discus hannai Ino	166:199	Three polysaccharides, AAP, AVAP I, and AVAP II, were isolated from abalone Haliotis discus hannai Ino.
24548747	1	40	attach	isolated	152:159	arg2	AAP					121:123	AAP	121:123	AAP	121:123	Three polysaccharides, AAP, AVAP I, and AVAP II, were isolated from abalone Haliotis discus hannai Ino.
24548747	1	40	attach	isolated	152:159	arg2	polysaccharides					104:118	Three polysaccharides	98:118	Three polysaccharides	98:118	Three polysaccharides, AAP, AVAP I, and AVAP II, were isolated from abalone Haliotis discus hannai Ino.
24548747	1	40	attach	isolated	152:159	arg2	II					143:144	AVAP II	138:144	AVAP II	138:144	Three polysaccharides, AAP, AVAP I, and AVAP II, were isolated from abalone Haliotis discus hannai Ino.
24548747	6	41	theme	cycle	904:908	arg1	process					910:916	the cell cycle process	895:916	the cell cycle process	895:916	AVAP I could therefore promote HepG2 cell proliferation by regulating gene expression and accelerating the cell cycle process.
24548747	2	42	theme	cell	278:281	arg1	proliferation					283:295	HepG2 cell proliferation	272:295	HepG2 cell proliferation	272:295	The polysaccharides' compositions were analysed, and their effects on HepG2 cell proliferation were assessed.
24548747	1	43	theme	Haliotis	174:181	arg1	Ino					197:199	abalone Haliotis discus hannai Ino	166:199	abalone Haliotis discus hannai Ino	166:199	Three polysaccharides, AAP, AVAP I, and AVAP II, were isolated from abalone Haliotis discus hannai Ino.
24548747	4	44	theme	same	437:440	arg1	effects					449:455	the same growth effects	433:455	the same growth effects	433:455	The oligosaccharide of AVAP I (Oli-AVAP I) exhibited the same growth effects, but rhamnose, the primary monosaccharide of AVAP I and Oli-AVAP I, did not exhibit this activity.
24548747	2	45	theme	HepG2	272:276	arg1	proliferation					283:295	HepG2 cell proliferation	272:295	HepG2 cell proliferation	272:295	The polysaccharides' compositions were analysed, and their effects on HepG2 cell proliferation were assessed.
24548747	1	46	theme	discus	183:188	arg1	Ino					197:199	abalone Haliotis discus hannai Ino	166:199	abalone Haliotis discus hannai Ino	166:199	Three polysaccharides, AAP, AVAP I, and AVAP II, were isolated from abalone Haliotis discus hannai Ino.
24548747	5	47	theme	cell	736:739	arg1	proliferation					741:753	cell proliferation	736:753	cell proliferation	736:753	Moreover, AVAP I dramatically reduced the mRNA levels of CDK6 and Cyclin E1 but significantly increased Cyclin B1, CDK1 and Cyclin F. Interestingly, AVAP I remained able to induce cell proliferation in a low serum concentration medium.
24548747	1	48	theme	hannai	190:195	arg1	Ino					197:199	abalone Haliotis discus hannai Ino	166:199	abalone Haliotis discus hannai Ino	166:199	Three polysaccharides, AAP, AVAP I, and AVAP II, were isolated from abalone Haliotis discus hannai Ino.
24548747	0	49	theme	hannai	57:62	arg1	Ino					64:66	Haliotis discus hannai Ino	41:66	Haliotis discus hannai Ino	41:66	Effects of polysaccharides from abalone (Haliotis discus hannai Ino) on HepG2 cell proliferation.
24548747	0	49	theme	hannai	57:62	arg1	abalone					32:38	abalone	32:38	abalone (Haliotis discus hannai Ino)	32:67	Effects of polysaccharides from abalone (Haliotis discus hannai Ino) on HepG2 cell proliferation.
24548747	1	50	theme	AVAP	126:129	arg1	I					131:131	AVAP I	126:131	AVAP I	126:131	Three polysaccharides, AAP, AVAP I, and AVAP II, were isolated from abalone Haliotis discus hannai Ino.
24548747	1	50	theme	AVAP	126:129	arg1	polysaccharides					104:118	Three polysaccharides	98:118	Three polysaccharides	98:118	Three polysaccharides, AAP, AVAP I, and AVAP II, were isolated from abalone Haliotis discus hannai Ino.
24548747	5	51	theme	Cyclin	622:627	arg1	E1					629:630	Cyclin E1	622:630	Cyclin E1	622:630	Moreover, AVAP I dramatically reduced the mRNA levels of CDK6 and Cyclin E1 but significantly increased Cyclin B1, CDK1 and Cyclin F. Interestingly, AVAP I remained able to induce cell proliferation in a low serum concentration medium.
24657410	0	0	theme	central	77:83	arg1	design					95:100	central composite design	77:100	central composite design	77:100	Optimization of a chromatographic stationary phase based on gellan gum using central composite design.
24657410	1	1	theme	divalent	323:330	arg1	cations					332:338	divalent cations	323:338	divalent cations	323:338	To develop a new stationary phase of easy production, low cost, biocompatible, biodegradable and low unspecific adsorption, a three-dimensional network was prepared by combining the natural polysaccharide of gellan with divalent cations.
24657410	9	2	dep	matrix	1439:1444	arg1	explore					1449:1455	explore	1449:1455	to explore ionic interactions	1446:1474	In this way, gellan gel might be a promising chromatographic matrix to explore ionic interactions and to be applied in different purification strategies, getting the best benefit from its use at low cost.
24657410	9	2	dep	matrix	1439:1444	arg1	applied					1486:1492	applied	1486:1492	to be applied in different purification strategies	1480:1529	In this way, gellan gel might be a promising chromatographic matrix to explore ionic interactions and to be applied in different purification strategies, getting the best benefit from its use at low cost.
24657410	1	3	theme	easy	140:143	arg1	production					145:154	easy production	140:154	easy production	140:154	To develop a new stationary phase of easy production, low cost, biocompatible, biodegradable and low unspecific adsorption, a three-dimensional network was prepared by combining the natural polysaccharide of gellan with divalent cations.
24657410	8	4	theme	breakthrough	1350:1361	arg1	breakthrough					1350:1361	breakthrough	1350:1361	breakthrough	1350:1361	The values of dynamic binding capacity from gellan gel were 3.9mg/mL and 17.4mg/mL, at 10% and 50% of breakthrough, respectively.
24657410	8	4	theme	breakthrough	1350:1361	arg1	%					1337:1337	10%	1335:1337	10%	1335:1337	The values of dynamic binding capacity from gellan gel were 3.9mg/mL and 17.4mg/mL, at 10% and 50% of breakthrough, respectively.
24657410	8	4	theme	breakthrough	1350:1361	arg1	%					1345:1345	50%	1343:1345	50% of breakthrough	1343:1361	The values of dynamic binding capacity from gellan gel were 3.9mg/mL and 17.4mg/mL, at 10% and 50% of breakthrough, respectively.
24657410	9	5	theme	different	1497:1505	arg1	strategies					1520:1529	different purification strategies	1497:1529	different purification strategies	1497:1529	In this way, gellan gel might be a promising chromatographic matrix to explore ionic interactions and to be applied in different purification strategies, getting the best benefit from its use at low cost.
24657410	5	6	theme	MES	824:826	arg1	pH					835:836	MES buffer pH 6.2	824:840	MES buffer pH 6.2	824:840	The results showed that the retention occurred in function of the net charge of each protein in MES buffer pH 6.2 and the elution was performed by increase of ionic strength to 750mM NaCl in MES buffer pH 6.2.
24657410	1	7	theme	production	145:154	arg1	adsorption					215:224	biocompatible, biodegradable and low unspecific adsorption	167:224	biocompatible, biodegradable and low unspecific adsorption	167:224	To develop a new stationary phase of easy production, low cost, biocompatible, biodegradable and low unspecific adsorption, a three-dimensional network was prepared by combining the natural polysaccharide of gellan with divalent cations.
24657410	1	7	theme	production	145:154	arg1	cost					161:164	low cost	157:164	low cost	157:164	To develop a new stationary phase of easy production, low cost, biocompatible, biodegradable and low unspecific adsorption, a three-dimensional network was prepared by combining the natural polysaccharide of gellan with divalent cations.
24657410	1	7	theme	production	145:154	arg1	phase					131:135	a new stationary phase	114:135	a new stationary phase of easy production	114:154	To develop a new stationary phase of easy production, low cost, biocompatible, biodegradable and low unspecific adsorption, a three-dimensional network was prepared by combining the natural polysaccharide of gellan with divalent cations.
24657410	1	8	theme	three-dimensional	229:245	arg1	network					247:253	a three-dimensional network	227:253	a three-dimensional network	227:253	To develop a new stationary phase of easy production, low cost, biocompatible, biodegradable and low unspecific adsorption, a three-dimensional network was prepared by combining the natural polysaccharide of gellan with divalent cations.
24657410	9	9	from	cost	1577:1580	arg1	use					1566:1568	its use	1562:1568	its use at low cost	1562:1580	In this way, gellan gel might be a promising chromatographic matrix to explore ionic interactions and to be applied in different purification strategies, getting the best benefit from its use at low cost.
24657410	9	10	theme	purification	1507:1518	arg1	strategies					1520:1529	different purification strategies	1497:1529	different purification strategies	1497:1529	In this way, gellan gel might be a promising chromatographic matrix to explore ionic interactions and to be applied in different purification strategies, getting the best benefit from its use at low cost.
24657410	4	11	dep	proteins	660:667	arg1	proteins					660:667	three model proteins	648:667	three model proteins (bovine serum albumin (BSA), α-chymotripsin and lysozyme)	648:725	The applicability of gellan matrix was tested by chromatographic assays with three model proteins (bovine serum albumin (BSA), α-chymotripsin and lysozyme).
24657410	4	11	dep	proteins	660:667	arg1	lysozyme					717:724	lysozyme	717:724	lysozyme	717:724	The applicability of gellan matrix was tested by chromatographic assays with three model proteins (bovine serum albumin (BSA), α-chymotripsin and lysozyme).
24657410	4	11	dep	proteins	660:667	arg1	BSA					692:694	BSA	692:694	BSA	692:694	The applicability of gellan matrix was tested by chromatographic assays with three model proteins (bovine serum albumin (BSA), α-chymotripsin and lysozyme).
24657410	4	11	dep	proteins	660:667	arg1	α-chymotripsin					698:711	α-chymotripsin	698:711	α-chymotripsin	698:711	The applicability of gellan matrix was tested by chromatographic assays with three model proteins (bovine serum albumin (BSA), α-chymotripsin and lysozyme).
24657410	4	11	dep	proteins	660:667	arg1	albumin					683:689	bovine serum albumin	670:689	bovine serum albumin (BSA)	670:695	The applicability of gellan matrix was tested by chromatographic assays with three model proteins (bovine serum albumin (BSA), α-chymotripsin and lysozyme).
24657410	8	12	theme	dynamic	1262:1268	arg1	capacity					1278:1285	dynamic binding capacity	1262:1285	dynamic binding capacity from gellan gel	1262:1301	The values of dynamic binding capacity from gellan gel were 3.9mg/mL and 17.4mg/mL, at 10% and 50% of breakthrough, respectively.
24657410	0	13	theme	composite	85:93	arg1	design					95:100	central composite design	77:100	central composite design	77:100	Optimization of a chromatographic stationary phase based on gellan gum using central composite design.
24657410	3	14	theme	gel	501:503	arg1	formulation					505:515	the gellan gel formulation	490:515	the gellan gel formulation	490:515	The optimal conditions proposed for the gellan gel formulation were 48mM ZnSO4, 0% DMF, 25°C, 0.75% gellan and 0.5h.
24657410	5	15	from	NaCl	911:914	arg1	pH					930:931	MES buffer pH 6.2	919:935	MES buffer pH 6.2	919:935	The results showed that the retention occurred in function of the net charge of each protein in MES buffer pH 6.2 and the elution was performed by increase of ionic strength to 750mM NaCl in MES buffer pH 6.2.
24657410	3	16	dep	%	535:535	arg1	DMF					537:539	DMF	537:539	48mM ZnSO4, 0% DMF	522:539	The optimal conditions proposed for the gellan gel formulation were 48mM ZnSO4, 0% DMF, 25°C, 0.75% gellan and 0.5h.
24657410	8	17	from	gel	1299:1301	arg1	3.9mg/mL					1308:1315	3.9mg/mL	1308:1315	3.9mg/mL	1308:1315	The values of dynamic binding capacity from gellan gel were 3.9mg/mL and 17.4mg/mL, at 10% and 50% of breakthrough, respectively.
24657410	8	17	from	gel	1299:1301	arg1	capacity					1278:1285	dynamic binding capacity	1262:1285	dynamic binding capacity from gellan gel	1262:1301	The values of dynamic binding capacity from gellan gel were 3.9mg/mL and 17.4mg/mL, at 10% and 50% of breakthrough, respectively.
24657410	8	17	from	gel	1299:1301	arg1	values					1252:1257	The values	1248:1257	The values of dynamic binding capacity from gellan gel	1248:1301	The values of dynamic binding capacity from gellan gel were 3.9mg/mL and 17.4mg/mL, at 10% and 50% of breakthrough, respectively.
24657410	6	18	theme	due	977:979	arg1	protein					969:975	the more retained protein	951:975	the more retained protein due to its positive charge	951:1002	Lysozyme was the more retained protein due to its positive charge more effective than α-chymotripsin, while BSA did not interact with the matrix due to its negative charge at these conditions.
24657410	6	18	theme	due	977:979	arg1	Lysozyme					938:945	Lysozyme	938:945	Lysozyme	938:945	Lysozyme was the more retained protein due to its positive charge more effective than α-chymotripsin, while BSA did not interact with the matrix due to its negative charge at these conditions.
24657410	3	19	theme	ZnSO4	527:531	arg1	%					535:535	48mM ZnSO4, 0%	522:535	%	535:535	The optimal conditions proposed for the gellan gel formulation were 48mM ZnSO4, 0% DMF, 25°C, 0.75% gellan and 0.5h.
24657410	3	19	theme	ZnSO4	527:531	arg1	conditions					466:475	The optimal conditions	454:475	The optimal conditions proposed for the gellan gel formulation	454:515	The optimal conditions proposed for the gellan gel formulation were 48mM ZnSO4, 0% DMF, 25°C, 0.75% gellan and 0.5h.
24657410	6	20	theme	retained	960:967	arg1	protein					969:975	the more retained protein	951:975	the more retained protein due to its positive charge	951:1002	Lysozyme was the more retained protein due to its positive charge more effective than α-chymotripsin, while BSA did not interact with the matrix due to its negative charge at these conditions.
24657410	6	20	theme	retained	960:967	arg1	Lysozyme					938:945	Lysozyme	938:945	Lysozyme	938:945	Lysozyme was the more retained protein due to its positive charge more effective than α-chymotripsin, while BSA did not interact with the matrix due to its negative charge at these conditions.
24657410	2	21	theme	matrix	395:400	arg1	stability					345:353	The stability	341:353	The stability of this cation exchange chromatographic matrix	341:400	The stability of this cation exchange chromatographic matrix was optimized by using an experimental design tool.
24657410	5	22	theme	buffer	828:833	arg1	pH					835:836	MES buffer pH 6.2	824:840	MES buffer pH 6.2	824:840	The results showed that the retention occurred in function of the net charge of each protein in MES buffer pH 6.2 and the elution was performed by increase of ionic strength to 750mM NaCl in MES buffer pH 6.2.
24657410	5	23	theme	strength	893:900	arg1	increase					875:882	increase	875:882	increase of ionic strength to 750mM NaCl in MES buffer pH 6.2	875:935	The results showed that the retention occurred in function of the net charge of each protein in MES buffer pH 6.2 and the elution was performed by increase of ionic strength to 750mM NaCl in MES buffer pH 6.2.
24657410	1	24	theme	low	157:159	arg1	cost					161:164	low cost	157:164	low cost	157:164	To develop a new stationary phase of easy production, low cost, biocompatible, biodegradable and low unspecific adsorption, a three-dimensional network was prepared by combining the natural polysaccharide of gellan with divalent cations.
24657410	1	24	theme	low	157:159	arg1	phase					131:135	a new stationary phase	114:135	a new stationary phase of easy production	114:154	To develop a new stationary phase of easy production, low cost, biocompatible, biodegradable and low unspecific adsorption, a three-dimensional network was prepared by combining the natural polysaccharide of gellan with divalent cations.
24657410	8	25	theme	capacity	1278:1285	arg1	3.9mg/mL					1308:1315	3.9mg/mL	1308:1315	3.9mg/mL	1308:1315	The values of dynamic binding capacity from gellan gel were 3.9mg/mL and 17.4mg/mL, at 10% and 50% of breakthrough, respectively.
24657410	8	25	theme	capacity	1278:1285	arg1	values					1252:1257	The values	1248:1257	The values of dynamic binding capacity from gellan gel	1248:1301	The values of dynamic binding capacity from gellan gel were 3.9mg/mL and 17.4mg/mL, at 10% and 50% of breakthrough, respectively.
24657410	7	26	theme	binding	1139:1145	arg1	assays					1156:1161	Dynamic binding capacity assays	1131:1161	Dynamic binding capacity assays	1131:1161	Dynamic binding capacity assays were accomplished to characterize this matrix and to compare with commercial resins.
24657410	9	27	from	matrix	1439:1444	arg1	way					1386:1388	this way	1381:1388	this way	1381:1388	In this way, gellan gel might be a promising chromatographic matrix to explore ionic interactions and to be applied in different purification strategies, getting the best benefit from its use at low cost.
24657410	2	28	theme	exchange	370:377	arg1	matrix					395:400	this cation exchange chromatographic matrix	358:400	this cation exchange chromatographic matrix	358:400	The stability of this cation exchange chromatographic matrix was optimized by using an experimental design tool.
24657410	2	29	theme	design	441:446	arg1	tool					448:451	an experimental design tool	425:451	an experimental design tool	425:451	The stability of this cation exchange chromatographic matrix was optimized by using an experimental design tool.
24657410	4	30	theme	serum	677:681	arg1	proteins					660:667	three model proteins	648:667	three model proteins (bovine serum albumin (BSA), α-chymotripsin and lysozyme)	648:725	The applicability of gellan matrix was tested by chromatographic assays with three model proteins (bovine serum albumin (BSA), α-chymotripsin and lysozyme).
24657410	4	30	theme	serum	677:681	arg1	BSA					692:694	BSA	692:694	BSA	692:694	The applicability of gellan matrix was tested by chromatographic assays with three model proteins (bovine serum albumin (BSA), α-chymotripsin and lysozyme).
24657410	4	30	theme	serum	677:681	arg1	albumin					683:689	bovine serum albumin	670:689	bovine serum albumin (BSA)	670:695	The applicability of gellan matrix was tested by chromatographic assays with three model proteins (bovine serum albumin (BSA), α-chymotripsin and lysozyme).
24657410	5	31	theme	net	794:796	arg1	charge					798:803	the net charge	790:803	the net charge of each protein	790:819	The results showed that the retention occurred in function of the net charge of each protein in MES buffer pH 6.2 and the elution was performed by increase of ionic strength to 750mM NaCl in MES buffer pH 6.2.
24657410	5	32	theme	charge	798:803	arg1	function					778:785	function	778:785	function of the net charge of each protein in MES buffer pH 6.2	778:840	The results showed that the retention occurred in function of the net charge of each protein in MES buffer pH 6.2 and the elution was performed by increase of ionic strength to 750mM NaCl in MES buffer pH 6.2.
24657410	2	33	theme	cation	363:368	arg1	matrix					395:400	this cation exchange chromatographic matrix	358:400	this cation exchange chromatographic matrix	358:400	The stability of this cation exchange chromatographic matrix was optimized by using an experimental design tool.
24657410	0	34	theme	chromatographic	18:32	arg1	phase					45:49	a chromatographic stationary phase	16:49	a chromatographic stationary phase	16:49	Optimization of a chromatographic stationary phase based on gellan gum using central composite design.
24657410	8	35	theme	gellan	1292:1297	arg1	gel					1299:1301	gellan gel	1292:1301	gellan gel	1292:1301	The values of dynamic binding capacity from gellan gel were 3.9mg/mL and 17.4mg/mL, at 10% and 50% of breakthrough, respectively.
24657410	8	36	theme	binding	1270:1276	arg1	capacity					1278:1285	dynamic binding capacity	1262:1285	dynamic binding capacity from gellan gel	1262:1301	The values of dynamic binding capacity from gellan gel were 3.9mg/mL and 17.4mg/mL, at 10% and 50% of breakthrough, respectively.
24657410	5	37	theme	MES	919:921	arg1	pH					930:931	MES buffer pH 6.2	919:935	MES buffer pH 6.2	919:935	The results showed that the retention occurred in function of the net charge of each protein in MES buffer pH 6.2 and the elution was performed by increase of ionic strength to 750mM NaCl in MES buffer pH 6.2.
24657410	1	38	theme	biocompatible	167:179	arg1	adsorption					215:224	biocompatible, biodegradable and low unspecific adsorption	167:224	biocompatible, biodegradable and low unspecific adsorption	167:224	To develop a new stationary phase of easy production, low cost, biocompatible, biodegradable and low unspecific adsorption, a three-dimensional network was prepared by combining the natural polysaccharide of gellan with divalent cations.
24657410	1	38	theme	biocompatible	167:179	arg1	phase					131:135	a new stationary phase	114:135	a new stationary phase of easy production	114:154	To develop a new stationary phase of easy production, low cost, biocompatible, biodegradable and low unspecific adsorption, a three-dimensional network was prepared by combining the natural polysaccharide of gellan with divalent cations.
24657410	9	39	theme	promising	1413:1421	arg1	gel					1398:1400	gellan gel	1391:1400	gellan gel	1391:1400	In this way, gellan gel might be a promising chromatographic matrix to explore ionic interactions and to be applied in different purification strategies, getting the best benefit from its use at low cost.
24657410	9	39	theme	promising	1413:1421	arg1	matrix					1439:1444	a promising chromatographic matrix	1411:1444	a promising chromatographic matrix to explore ionic interactions and to be applied in different purification strategies	1411:1529	In this way, gellan gel might be a promising chromatographic matrix to explore ionic interactions and to be applied in different purification strategies, getting the best benefit from its use at low cost.
24657410	5	40	theme	ionic	887:891	arg1	strength					893:900	ionic strength	887:900	ionic strength to 750mM NaCl in MES buffer pH 6.2	887:935	The results showed that the retention occurred in function of the net charge of each protein in MES buffer pH 6.2 and the elution was performed by increase of ionic strength to 750mM NaCl in MES buffer pH 6.2.
24657410	4	41	with	assays	636:641	arg1	proteins					660:667	three model proteins	648:667	three model proteins (bovine serum albumin (BSA), α-chymotripsin and lysozyme)	648:725	The applicability of gellan matrix was tested by chromatographic assays with three model proteins (bovine serum albumin (BSA), α-chymotripsin and lysozyme).
24657410	4	41	with	assays	636:641	arg1	lysozyme					717:724	lysozyme	717:724	lysozyme	717:724	The applicability of gellan matrix was tested by chromatographic assays with three model proteins (bovine serum albumin (BSA), α-chymotripsin and lysozyme).
24657410	4	41	with	assays	636:641	arg1	α-chymotripsin					698:711	α-chymotripsin	698:711	α-chymotripsin	698:711	The applicability of gellan matrix was tested by chromatographic assays with three model proteins (bovine serum albumin (BSA), α-chymotripsin and lysozyme).
24657410	4	41	with	assays	636:641	arg1	albumin					683:689	bovine serum albumin	670:689	bovine serum albumin (BSA)	670:695	The applicability of gellan matrix was tested by chromatographic assays with three model proteins (bovine serum albumin (BSA), α-chymotripsin and lysozyme).
24657410	4	42	theme	model	654:658	arg1	proteins					660:667	three model proteins	648:667	three model proteins (bovine serum albumin (BSA), α-chymotripsin and lysozyme)	648:725	The applicability of gellan matrix was tested by chromatographic assays with three model proteins (bovine serum albumin (BSA), α-chymotripsin and lysozyme).
24657410	4	42	theme	model	654:658	arg1	lysozyme					717:724	lysozyme	717:724	lysozyme	717:724	The applicability of gellan matrix was tested by chromatographic assays with three model proteins (bovine serum albumin (BSA), α-chymotripsin and lysozyme).
24657410	4	42	theme	model	654:658	arg1	α-chymotripsin					698:711	α-chymotripsin	698:711	α-chymotripsin	698:711	The applicability of gellan matrix was tested by chromatographic assays with three model proteins (bovine serum albumin (BSA), α-chymotripsin and lysozyme).
24657410	4	42	theme	model	654:658	arg1	albumin					683:689	bovine serum albumin	670:689	bovine serum albumin (BSA)	670:695	The applicability of gellan matrix was tested by chromatographic assays with three model proteins (bovine serum albumin (BSA), α-chymotripsin and lysozyme).
24657410	7	43	theme	Dynamic	1131:1137	arg1	assays					1156:1161	Dynamic binding capacity assays	1131:1161	Dynamic binding capacity assays	1131:1161	Dynamic binding capacity assays were accomplished to characterize this matrix and to compare with commercial resins.
24657410	0	44	theme	phase	45:49	arg1	Optimization					0:11	Optimization	0:11	Optimization of a chromatographic stationary phase	0:49	Optimization of a chromatographic stationary phase based on gellan gum using central composite design.
24657410	9	45	theme	best	1544:1547	arg1	benefit					1549:1555	the best benefit	1540:1555	the best benefit from its use at low cost	1540:1580	In this way, gellan gel might be a promising chromatographic matrix to explore ionic interactions and to be applied in different purification strategies, getting the best benefit from its use at low cost.
24657410	3	46	theme	gellan	554:559	arg1	%					552:552	0.75% gellan	548:559	0.75% gellan	548:559	The optimal conditions proposed for the gellan gel formulation were 48mM ZnSO4, 0% DMF, 25°C, 0.75% gellan and 0.5h.
24657410	4	47	theme	chromatographic	620:634	arg1	assays					636:641	chromatographic assays	620:641	chromatographic assays with three model proteins (bovine serum albumin (BSA), α-chymotripsin and lysozyme)	620:725	The applicability of gellan matrix was tested by chromatographic assays with three model proteins (bovine serum albumin (BSA), α-chymotripsin and lysozyme).
24657410	1	48	theme	biodegradable	182:194	arg1	adsorption					215:224	biocompatible, biodegradable and low unspecific adsorption	167:224	biocompatible, biodegradable and low unspecific adsorption	167:224	To develop a new stationary phase of easy production, low cost, biocompatible, biodegradable and low unspecific adsorption, a three-dimensional network was prepared by combining the natural polysaccharide of gellan with divalent cations.
24657410	1	48	theme	biodegradable	182:194	arg1	phase					131:135	a new stationary phase	114:135	a new stationary phase of easy production	114:154	To develop a new stationary phase of easy production, low cost, biocompatible, biodegradable and low unspecific adsorption, a three-dimensional network was prepared by combining the natural polysaccharide of gellan with divalent cations.
24657410	0	49	theme	stationary	34:43	arg1	phase					45:49	a chromatographic stationary phase	16:49	a chromatographic stationary phase	16:49	Optimization of a chromatographic stationary phase based on gellan gum using central composite design.
24657410	1	50	theme	natural	285:291	arg1	polysaccharide					293:306	the natural polysaccharide	281:306	the natural polysaccharide of gellan	281:316	To develop a new stationary phase of easy production, low cost, biocompatible, biodegradable and low unspecific adsorption, a three-dimensional network was prepared by combining the natural polysaccharide of gellan with divalent cations.
24657410	6	51	from	conditions	1119:1128	arg1	charge					1103:1108	its negative charge	1090:1108	its negative charge at these conditions	1090:1128	Lysozyme was the more retained protein due to its positive charge more effective than α-chymotripsin, while BSA did not interact with the matrix due to its negative charge at these conditions.
24657410	2	52	theme	experimental	428:439	arg1	tool					448:451	an experimental design tool	425:451	an experimental design tool	425:451	The stability of this cation exchange chromatographic matrix was optimized by using an experimental design tool.
24657410	4	53	theme	matrix	599:604	arg1	applicability					575:587	The applicability	571:587	The applicability of gellan matrix	571:604	The applicability of gellan matrix was tested by chromatographic assays with three model proteins (bovine serum albumin (BSA), α-chymotripsin and lysozyme).
24657410	9	54	theme	ionic	1457:1461	arg1	interactions					1463:1474	ionic interactions	1457:1474	ionic interactions	1457:1474	In this way, gellan gel might be a promising chromatographic matrix to explore ionic interactions and to be applied in different purification strategies, getting the best benefit from its use at low cost.
24657410	4	55	theme	bovine	670:675	arg1	proteins					660:667	three model proteins	648:667	three model proteins (bovine serum albumin (BSA), α-chymotripsin and lysozyme)	648:725	The applicability of gellan matrix was tested by chromatographic assays with three model proteins (bovine serum albumin (BSA), α-chymotripsin and lysozyme).
24657410	4	55	theme	bovine	670:675	arg1	BSA					692:694	BSA	692:694	BSA	692:694	The applicability of gellan matrix was tested by chromatographic assays with three model proteins (bovine serum albumin (BSA), α-chymotripsin and lysozyme).
24657410	4	55	theme	bovine	670:675	arg1	albumin					683:689	bovine serum albumin	670:689	bovine serum albumin (BSA)	670:695	The applicability of gellan matrix was tested by chromatographic assays with three model proteins (bovine serum albumin (BSA), α-chymotripsin and lysozyme).
24657410	9	56	theme	low	1573:1575	arg1	cost					1577:1580	low cost	1573:1580	low cost	1573:1580	In this way, gellan gel might be a promising chromatographic matrix to explore ionic interactions and to be applied in different purification strategies, getting the best benefit from its use at low cost.
24657410	9	57	theme	gellan	1391:1396	arg1	gel					1398:1400	gellan gel	1391:1400	gellan gel	1391:1400	In this way, gellan gel might be a promising chromatographic matrix to explore ionic interactions and to be applied in different purification strategies, getting the best benefit from its use at low cost.
24657410	9	57	theme	gellan	1391:1396	arg1	matrix					1439:1444	a promising chromatographic matrix	1411:1444	a promising chromatographic matrix to explore ionic interactions and to be applied in different purification strategies	1411:1529	In this way, gellan gel might be a promising chromatographic matrix to explore ionic interactions and to be applied in different purification strategies, getting the best benefit from its use at low cost.
24657410	6	58	theme	negative	1094:1101	arg1	charge					1103:1108	its negative charge	1090:1108	its negative charge at these conditions	1090:1128	Lysozyme was the more retained protein due to its positive charge more effective than α-chymotripsin, while BSA did not interact with the matrix due to its negative charge at these conditions.
24657410	9	59	from	use	1566:1568	arg1	benefit					1549:1555	the best benefit	1540:1555	the best benefit from its use at low cost	1540:1580	In this way, gellan gel might be a promising chromatographic matrix to explore ionic interactions and to be applied in different purification strategies, getting the best benefit from its use at low cost.
24657410	3	60	theme	gellan	494:499	arg1	formulation					505:515	the gellan gel formulation	490:515	the gellan gel formulation	490:515	The optimal conditions proposed for the gellan gel formulation were 48mM ZnSO4, 0% DMF, 25°C, 0.75% gellan and 0.5h.
24657410	1	61	theme	gellan	311:316	arg1	polysaccharide					293:306	the natural polysaccharide	281:306	the natural polysaccharide of gellan	281:316	To develop a new stationary phase of easy production, low cost, biocompatible, biodegradable and low unspecific adsorption, a three-dimensional network was prepared by combining the natural polysaccharide of gellan with divalent cations.
24657410	6	62	dep	effective	1009:1017	arg1	protein					969:975	the more retained protein	951:975	the more retained protein due to its positive charge	951:1002	Lysozyme was the more retained protein due to its positive charge more effective than α-chymotripsin, while BSA did not interact with the matrix due to its negative charge at these conditions.
24657410	6	62	dep	effective	1009:1017	arg1	Lysozyme					938:945	Lysozyme	938:945	Lysozyme	938:945	Lysozyme was the more retained protein due to its positive charge more effective than α-chymotripsin, while BSA did not interact with the matrix due to its negative charge at these conditions.
24657410	5	63	theme	buffer	923:928	arg1	pH					930:931	MES buffer pH 6.2	919:935	MES buffer pH 6.2	919:935	The results showed that the retention occurred in function of the net charge of each protein in MES buffer pH 6.2 and the elution was performed by increase of ionic strength to 750mM NaCl in MES buffer pH 6.2.
24657410	1	64	theme	new	116:118	arg1	adsorption					215:224	biocompatible, biodegradable and low unspecific adsorption	167:224	biocompatible, biodegradable and low unspecific adsorption	167:224	To develop a new stationary phase of easy production, low cost, biocompatible, biodegradable and low unspecific adsorption, a three-dimensional network was prepared by combining the natural polysaccharide of gellan with divalent cations.
24657410	1	64	theme	new	116:118	arg1	cost					161:164	low cost	157:164	low cost	157:164	To develop a new stationary phase of easy production, low cost, biocompatible, biodegradable and low unspecific adsorption, a three-dimensional network was prepared by combining the natural polysaccharide of gellan with divalent cations.
24657410	1	64	theme	new	116:118	arg1	phase					131:135	a new stationary phase	114:135	a new stationary phase of easy production	114:154	To develop a new stationary phase of easy production, low cost, biocompatible, biodegradable and low unspecific adsorption, a three-dimensional network was prepared by combining the natural polysaccharide of gellan with divalent cations.
24657410	9	65	theme	chromatographic	1423:1437	arg1	gel					1398:1400	gellan gel	1391:1400	gellan gel	1391:1400	In this way, gellan gel might be a promising chromatographic matrix to explore ionic interactions and to be applied in different purification strategies, getting the best benefit from its use at low cost.
24657410	9	65	theme	chromatographic	1423:1437	arg1	matrix					1439:1444	a promising chromatographic matrix	1411:1444	a promising chromatographic matrix to explore ionic interactions and to be applied in different purification strategies	1411:1529	In this way, gellan gel might be a promising chromatographic matrix to explore ionic interactions and to be applied in different purification strategies, getting the best benefit from its use at low cost.
24657410	1	66	theme	low	200:202	arg1	adsorption					215:224	biocompatible, biodegradable and low unspecific adsorption	167:224	biocompatible, biodegradable and low unspecific adsorption	167:224	To develop a new stationary phase of easy production, low cost, biocompatible, biodegradable and low unspecific adsorption, a three-dimensional network was prepared by combining the natural polysaccharide of gellan with divalent cations.
24657410	1	66	theme	low	200:202	arg1	phase					131:135	a new stationary phase	114:135	a new stationary phase of easy production	114:154	To develop a new stationary phase of easy production, low cost, biocompatible, biodegradable and low unspecific adsorption, a three-dimensional network was prepared by combining the natural polysaccharide of gellan with divalent cations.
24657410	5	67	from	function	778:785	arg1	pH					835:836	MES buffer pH 6.2	824:840	MES buffer pH 6.2	824:840	The results showed that the retention occurred in function of the net charge of each protein in MES buffer pH 6.2 and the elution was performed by increase of ionic strength to 750mM NaCl in MES buffer pH 6.2.
24657410	5	68	theme	750mM	905:909	arg1	NaCl					911:914	750mM NaCl	905:914	750mM NaCl in MES buffer pH 6.2	905:935	The results showed that the retention occurred in function of the net charge of each protein in MES buffer pH 6.2 and the elution was performed by increase of ionic strength to 750mM NaCl in MES buffer pH 6.2.
24657410	1	69	theme	stationary	120:129	arg1	adsorption					215:224	biocompatible, biodegradable and low unspecific adsorption	167:224	biocompatible, biodegradable and low unspecific adsorption	167:224	To develop a new stationary phase of easy production, low cost, biocompatible, biodegradable and low unspecific adsorption, a three-dimensional network was prepared by combining the natural polysaccharide of gellan with divalent cations.
24657410	1	69	theme	stationary	120:129	arg1	cost					161:164	low cost	157:164	low cost	157:164	To develop a new stationary phase of easy production, low cost, biocompatible, biodegradable and low unspecific adsorption, a three-dimensional network was prepared by combining the natural polysaccharide of gellan with divalent cations.
24657410	1	69	theme	stationary	120:129	arg1	phase					131:135	a new stationary phase	114:135	a new stationary phase of easy production	114:154	To develop a new stationary phase of easy production, low cost, biocompatible, biodegradable and low unspecific adsorption, a three-dimensional network was prepared by combining the natural polysaccharide of gellan with divalent cations.
24657410	1	70	theme	unspecific	204:213	arg1	adsorption					215:224	biocompatible, biodegradable and low unspecific adsorption	167:224	biocompatible, biodegradable and low unspecific adsorption	167:224	To develop a new stationary phase of easy production, low cost, biocompatible, biodegradable and low unspecific adsorption, a three-dimensional network was prepared by combining the natural polysaccharide of gellan with divalent cations.
24657410	1	70	theme	unspecific	204:213	arg1	phase					131:135	a new stationary phase	114:135	a new stationary phase of easy production	114:154	To develop a new stationary phase of easy production, low cost, biocompatible, biodegradable and low unspecific adsorption, a three-dimensional network was prepared by combining the natural polysaccharide of gellan with divalent cations.
24657410	8	71	from	%	1337:1337	arg1	3.9mg/mL					1308:1315	3.9mg/mL	1308:1315	3.9mg/mL	1308:1315	The values of dynamic binding capacity from gellan gel were 3.9mg/mL and 17.4mg/mL, at 10% and 50% of breakthrough, respectively.
24657410	8	71	from	%	1337:1337	arg1	values					1252:1257	The values	1248:1257	The values of dynamic binding capacity from gellan gel	1248:1301	The values of dynamic binding capacity from gellan gel were 3.9mg/mL and 17.4mg/mL, at 10% and 50% of breakthrough, respectively.
24657410	8	71	from	%	1337:1337	arg1	17.4mg/mL					1321:1329	17.4mg/mL	1321:1329	17.4mg/mL	1321:1329	The values of dynamic binding capacity from gellan gel were 3.9mg/mL and 17.4mg/mL, at 10% and 50% of breakthrough, respectively.
24657410	8	72	from	values	1252:1257	arg1	gel					1299:1301	gellan gel	1292:1301	gellan gel	1292:1301	The values of dynamic binding capacity from gellan gel were 3.9mg/mL and 17.4mg/mL, at 10% and 50% of breakthrough, respectively.
24657410	7	73	theme	commercial	1229:1238	arg1	resins					1240:1245	commercial resins	1229:1245	commercial resins	1229:1245	Dynamic binding capacity assays were accomplished to characterize this matrix and to compare with commercial resins.
24657410	3	74	theme	optimal	458:464	arg1	%					535:535	48mM ZnSO4, 0%	522:535	%	535:535	The optimal conditions proposed for the gellan gel formulation were 48mM ZnSO4, 0% DMF, 25°C, 0.75% gellan and 0.5h.
24657410	3	74	theme	optimal	458:464	arg1	conditions					466:475	The optimal conditions	454:475	The optimal conditions proposed for the gellan gel formulation	454:515	The optimal conditions proposed for the gellan gel formulation were 48mM ZnSO4, 0% DMF, 25°C, 0.75% gellan and 0.5h.
24657410	5	75	theme	protein	813:819	arg1	charge					798:803	the net charge	790:803	the net charge of each protein	790:819	The results showed that the retention occurred in function of the net charge of each protein in MES buffer pH 6.2 and the elution was performed by increase of ionic strength to 750mM NaCl in MES buffer pH 6.2.
24657410	2	76	theme	chromatographic	379:393	arg1	matrix					395:400	this cation exchange chromatographic matrix	358:400	this cation exchange chromatographic matrix	358:400	The stability of this cation exchange chromatographic matrix was optimized by using an experimental design tool.
24657410	0	77	theme	gellan	60:65	arg1	gum					67:69	gellan gum	60:69	gellan gum using central composite design	60:100	Optimization of a chromatographic stationary phase based on gellan gum using central composite design.
24657410	4	78	theme	gellan	592:597	arg1	matrix					599:604	gellan matrix	592:604	gellan matrix	592:604	The applicability of gellan matrix was tested by chromatographic assays with three model proteins (bovine serum albumin (BSA), α-chymotripsin and lysozyme).
24657410	7	79	theme	capacity	1147:1154	arg1	assays					1156:1161	Dynamic binding capacity assays	1131:1161	Dynamic binding capacity assays	1131:1161	Dynamic binding capacity assays were accomplished to characterize this matrix and to compare with commercial resins.
24657410	6	80	theme	positive	988:995	arg1	charge					997:1002	its positive charge	984:1002	its positive charge	984:1002	Lysozyme was the more retained protein due to its positive charge more effective than α-chymotripsin, while BSA did not interact with the matrix due to its negative charge at these conditions.
24657410	8	81	from	%	1345:1345	arg1	3.9mg/mL					1308:1315	3.9mg/mL	1308:1315	3.9mg/mL	1308:1315	The values of dynamic binding capacity from gellan gel were 3.9mg/mL and 17.4mg/mL, at 10% and 50% of breakthrough, respectively.
24657410	8	81	from	%	1345:1345	arg1	values					1252:1257	The values	1248:1257	The values of dynamic binding capacity from gellan gel	1248:1301	The values of dynamic binding capacity from gellan gel were 3.9mg/mL and 17.4mg/mL, at 10% and 50% of breakthrough, respectively.
24657410	8	81	from	%	1345:1345	arg1	17.4mg/mL					1321:1329	17.4mg/mL	1321:1329	17.4mg/mL	1321:1329	The values of dynamic binding capacity from gellan gel were 3.9mg/mL and 17.4mg/mL, at 10% and 50% of breakthrough, respectively.
24727271	0	0	theme	Svalbard	99:106	arg1	fjord					90:94	an arctic fjord	80:94	an arctic fjord of Svalbard	80:106	Verrucomicrobia are candidates for polysaccharide-degrading bacterioplankton in an arctic fjord of Svalbard.
24727271	0	1	from	candidates	20:29	arg1	fjord					90:94	an arctic fjord	80:94	an arctic fjord of Svalbard	80:106	Verrucomicrobia are candidates for polysaccharide-degrading bacterioplankton in an arctic fjord of Svalbard.
24727271	1	2	theme	phylum	164:169	arg1	Verrucomicrobia					171:185	the phylum Verrucomicrobia	160:185	the phylum Verrucomicrobia	160:185	In Arctic marine bacterial communities, members of the phylum Verrucomicrobia are consistently detected, although not typically abundant, in 16S rRNA gene clone libraries and pyrotag surveys of the marine water column and in sediments.
24727271	4	3	theme	sequenced	944:952	arg1	genomes					980:986	previously sequenced, glycoside hydrolase-rich genomes	933:986	genomes	980:986	Several Verrucomicrobia water column phylotypes were affiliated with previously sequenced, glycoside hydrolase-rich genomes of individual Verrucomicrobia cells that bound fluorescently labeled laminarin and xylan and therefore constituted candidates for laminarin and xylan hydrolysis.
24727271	1	4	theme	gene	259:262	arg1	libraries					270:278	16S rRNA gene clone libraries	250:278	16S rRNA gene clone libraries	250:278	In Arctic marine bacterial communities, members of the phylum Verrucomicrobia are consistently detected, although not typically abundant, in 16S rRNA gene clone libraries and pyrotag surveys of the marine water column and in sediments.
24727271	4	5	theme	hydrolase-rich	965:978	arg1	genomes					980:986	previously sequenced, glycoside hydrolase-rich genomes	933:986	genomes	980:986	Several Verrucomicrobia water column phylotypes were affiliated with previously sequenced, glycoside hydrolase-rich genomes of individual Verrucomicrobia cells that bound fluorescently labeled laminarin and xylan and therefore constituted candidates for laminarin and xylan hydrolysis.
24727271	3	6	dep	sulfate	787:793	arg1	hydrolysis					817:826	hydrolysis	817:826	hydrolysis	817:826	Parallel measurements in the water column of the activities of six endo-acting polysaccharide hydrolases showed that chondroitin sulfate, laminarin, and xylan hydrolysis accounted for most of the activity.
24727271	2	7	theme	library	620:626	arg1	analyses					628:635	16S rRNA gene-based clone library analyses	594:635	16S rRNA gene-based clone library analyses of the water column	594:655	In an Arctic fjord (Smeerenburgfjord) of Svalbard, members of the Verrucomicrobia, together with Flavobacteria and smaller proportions of Alpha- and Gammaproteobacteria, constituted the most frequently detected bacterioplankton community members in 16S rRNA gene-based clone library analyses of the water column.
24727271	2	8	theme	Alpha-	483:488	arg1	Flavobacteria					442:454	Flavobacteria	442:454	Flavobacteria	442:454	In an Arctic fjord (Smeerenburgfjord) of Svalbard, members of the Verrucomicrobia, together with Flavobacteria and smaller proportions of Alpha- and Gammaproteobacteria, constituted the most frequently detected bacterioplankton community members in 16S rRNA gene-based clone library analyses of the water column.
24727271	2	8	theme	Alpha-	483:488	arg1	proportions					468:478	smaller proportions	460:478	smaller proportions of Alpha- and Gammaproteobacteria	460:512	In an Arctic fjord (Smeerenburgfjord) of Svalbard, members of the Verrucomicrobia, together with Flavobacteria and smaller proportions of Alpha- and Gammaproteobacteria, constituted the most frequently detected bacterioplankton community members in 16S rRNA gene-based clone library analyses of the water column.
24727271	1	9	theme	Verrucomicrobia	171:185	arg1	members					149:155	members	149:155	members of the phylum Verrucomicrobia	149:185	In Arctic marine bacterial communities, members of the phylum Verrucomicrobia are consistently detected, although not typically abundant, in 16S rRNA gene clone libraries and pyrotag surveys of the marine water column and in sediments.
24727271	1	10	theme	clone	264:268	arg1	libraries					270:278	16S rRNA gene clone libraries	250:278	16S rRNA gene clone libraries	250:278	In Arctic marine bacterial communities, members of the phylum Verrucomicrobia are consistently detected, although not typically abundant, in 16S rRNA gene clone libraries and pyrotag surveys of the marine water column and in sediments.
24727271	4	11	theme	cells	1018:1022	arg1	genomes					980:986	previously sequenced, glycoside hydrolase-rich genomes	933:986	genomes	980:986	Several Verrucomicrobia water column phylotypes were affiliated with previously sequenced, glycoside hydrolase-rich genomes of individual Verrucomicrobia cells that bound fluorescently labeled laminarin and xylan and therefore constituted candidates for laminarin and xylan hydrolysis.
24727271	3	12	theme	activities	707:716	arg1	column					693:698	the water column	683:698	the water column of the activities of six endo-acting polysaccharide hydrolases	683:761	Parallel measurements in the water column of the activities of six endo-acting polysaccharide hydrolases showed that chondroitin sulfate, laminarin, and xylan hydrolysis accounted for most of the activity.
24727271	2	13	theme	clone	614:618	arg1	library					620:626	16S rRNA gene-based clone library	594:626	16S rRNA gene-based clone library analyses of the water column	594:655	In an Arctic fjord (Smeerenburgfjord) of Svalbard, members of the Verrucomicrobia, together with Flavobacteria and smaller proportions of Alpha- and Gammaproteobacteria, constituted the most frequently detected bacterioplankton community members in 16S rRNA gene-based clone library analyses of the water column.
24727271	5	14	theme	bacterial	1168:1176	arg1	community					1178:1186	the bacterial community	1164:1186	the bacterial community	1164:1186	In sediments, the bacterial community was dominated by different lineages of Verrucomicrobia, Bacteroidetes, and Proteobacteria but also included members of multiple phylum-level lineages not observed in the water column.
24727271	5	15	theme	multiple	1307:1314	arg1	lineages					1329:1336	multiple phylum-level lineages	1307:1336	multiple phylum-level lineages not observed in the water column	1307:1369	In sediments, the bacterial community was dominated by different lineages of Verrucomicrobia, Bacteroidetes, and Proteobacteria but also included members of multiple phylum-level lineages not observed in the water column.
24727271	4	16	theme	Verrucomicrobia	1002:1016	arg1	cells					1018:1022	individual Verrucomicrobia cells	991:1022	individual Verrucomicrobia cells that bound fluorescently labeled laminarin and xylan and therefore constituted candidates for laminarin and xylan hydrolysis	991:1147	Several Verrucomicrobia water column phylotypes were affiliated with previously sequenced, glycoside hydrolase-rich genomes of individual Verrucomicrobia cells that bound fluorescently labeled laminarin and xylan and therefore constituted candidates for laminarin and xylan hydrolysis.
24727271	3	17	theme	hydrolases	752:761	arg1	activities					707:716	the activities	703:716	the activities of six endo-acting polysaccharide hydrolases	703:761	Parallel measurements in the water column of the activities of six endo-acting polysaccharide hydrolases showed that chondroitin sulfate, laminarin, and xylan hydrolysis accounted for most of the activity.
24727271	2	18	theme	gene-based	603:612	arg1	library					620:626	16S rRNA gene-based clone library	594:626	16S rRNA gene-based clone library analyses of the water column	594:655	In an Arctic fjord (Smeerenburgfjord) of Svalbard, members of the Verrucomicrobia, together with Flavobacteria and smaller proportions of Alpha- and Gammaproteobacteria, constituted the most frequently detected bacterioplankton community members in 16S rRNA gene-based clone library analyses of the water column.
24727271	2	19	theme	Arctic	351:356	arg1	Smeerenburgfjord					365:380	Smeerenburgfjord	365:380	Smeerenburgfjord	365:380	In an Arctic fjord (Smeerenburgfjord) of Svalbard, members of the Verrucomicrobia, together with Flavobacteria and smaller proportions of Alpha- and Gammaproteobacteria, constituted the most frequently detected bacterioplankton community members in 16S rRNA gene-based clone library analyses of the water column.
24727271	2	19	theme	Arctic	351:356	arg1	fjord					358:362	an Arctic fjord	348:362	an Arctic fjord (Smeerenburgfjord) of Svalbard	348:393	In an Arctic fjord (Smeerenburgfjord) of Svalbard, members of the Verrucomicrobia, together with Flavobacteria and smaller proportions of Alpha- and Gammaproteobacteria, constituted the most frequently detected bacterioplankton community members in 16S rRNA gene-based clone library analyses of the water column.
24727271	5	20	theme	phylum-level	1316:1327	arg1	lineages					1329:1336	multiple phylum-level lineages	1307:1336	multiple phylum-level lineages not observed in the water column	1307:1369	In sediments, the bacterial community was dominated by different lineages of Verrucomicrobia, Bacteroidetes, and Proteobacteria but also included members of multiple phylum-level lineages not observed in the water column.
24727271	3	21	from	measurements	667:678	arg1	column					693:698	the water column	683:698	the water column of the activities of six endo-acting polysaccharide hydrolases	683:761	Parallel measurements in the water column of the activities of six endo-acting polysaccharide hydrolases showed that chondroitin sulfate, laminarin, and xylan hydrolysis accounted for most of the activity.
24727271	2	22	theme	column	650:655	arg1	analyses					628:635	16S rRNA gene-based clone library analyses	594:635	16S rRNA gene-based clone library analyses of the water column	594:655	In an Arctic fjord (Smeerenburgfjord) of Svalbard, members of the Verrucomicrobia, together with Flavobacteria and smaller proportions of Alpha- and Gammaproteobacteria, constituted the most frequently detected bacterioplankton community members in 16S rRNA gene-based clone library analyses of the water column.
24727271	2	23	dep	constituted	515:525	arg1	detected					547:554	detected	547:554	constituted the most frequently detected bacterioplankton community members in 16S rRNA gene-based clone library analyses of the water column	515:655	In an Arctic fjord (Smeerenburgfjord) of Svalbard, members of the Verrucomicrobia, together with Flavobacteria and smaller proportions of Alpha- and Gammaproteobacteria, constituted the most frequently detected bacterioplankton community members in 16S rRNA gene-based clone library analyses of the water column.
24727271	7	24	theme	same	1530:1533	arg1	fjord					1535:1539	the same fjord	1526:1539	the same fjord in the previous summer	1526:1562	Comparisons with data from the same fjord in the previous summer showed that the bacterial community in Smeerenburgfjord changed in composition, most conspicuously in the changing detection frequency of Verrucomicrobia in the water column.
24727271	7	25	theme	water	1725:1729	arg1	column					1731:1736	the water column	1721:1736	the water column	1721:1736	Comparisons with data from the same fjord in the previous summer showed that the bacterial community in Smeerenburgfjord changed in composition, most conspicuously in the changing detection frequency of Verrucomicrobia in the water column.
24727271	2	26	theme	Svalbard	386:393	arg1	Smeerenburgfjord					365:380	Smeerenburgfjord	365:380	Smeerenburgfjord	365:380	In an Arctic fjord (Smeerenburgfjord) of Svalbard, members of the Verrucomicrobia, together with Flavobacteria and smaller proportions of Alpha- and Gammaproteobacteria, constituted the most frequently detected bacterioplankton community members in 16S rRNA gene-based clone library analyses of the water column.
24727271	2	26	theme	Svalbard	386:393	arg1	fjord					358:362	an Arctic fjord	348:362	an Arctic fjord (Smeerenburgfjord) of Svalbard	348:393	In an Arctic fjord (Smeerenburgfjord) of Svalbard, members of the Verrucomicrobia, together with Flavobacteria and smaller proportions of Alpha- and Gammaproteobacteria, constituted the most frequently detected bacterioplankton community members in 16S rRNA gene-based clone library analyses of the water column.
24727271	6	27	theme	additional	1447:1456	arg1	substrates					1473:1482	three additional polysaccharide substrates	1441:1482	three additional polysaccharide substrates	1441:1482	This community hydrolyzed laminarin, xylan, chondroitin sulfate, and three additional polysaccharide substrates at high rates.
24727271	3	28	theme	endo-acting	725:735	arg1	hydrolases					752:761	six endo-acting polysaccharide hydrolases	721:761	six endo-acting polysaccharide hydrolases	721:761	Parallel measurements in the water column of the activities of six endo-acting polysaccharide hydrolases showed that chondroitin sulfate, laminarin, and xylan hydrolysis accounted for most of the activity.
24727271	2	29	theme	water	644:648	arg1	column					650:655	the water column	640:655	the water column	640:655	In an Arctic fjord (Smeerenburgfjord) of Svalbard, members of the Verrucomicrobia, together with Flavobacteria and smaller proportions of Alpha- and Gammaproteobacteria, constituted the most frequently detected bacterioplankton community members in 16S rRNA gene-based clone library analyses of the water column.
24727271	7	30	theme	detection	1679:1687	arg1	frequency					1689:1697	the changing detection frequency	1666:1697	the changing detection frequency of Verrucomicrobia in the water column	1666:1736	Comparisons with data from the same fjord in the previous summer showed that the bacterial community in Smeerenburgfjord changed in composition, most conspicuously in the changing detection frequency of Verrucomicrobia in the water column.
24727271	6	31	theme	polysaccharide	1458:1471	arg1	substrates					1473:1482	three additional polysaccharide substrates	1441:1482	three additional polysaccharide substrates	1441:1482	This community hydrolyzed laminarin, xylan, chondroitin sulfate, and three additional polysaccharide substrates at high rates.
24727271	3	32	theme	polysaccharide	737:750	arg1	hydrolases					752:761	six endo-acting polysaccharide hydrolases	721:761	six endo-acting polysaccharide hydrolases	721:761	Parallel measurements in the water column of the activities of six endo-acting polysaccharide hydrolases showed that chondroitin sulfate, laminarin, and xylan hydrolysis accounted for most of the activity.
24727271	4	33	theme	column	894:899	arg1	phylotypes					901:910	Several Verrucomicrobia water column phylotypes	864:910	Several Verrucomicrobia water column phylotypes	864:910	Several Verrucomicrobia water column phylotypes were affiliated with previously sequenced, glycoside hydrolase-rich genomes of individual Verrucomicrobia cells that bound fluorescently labeled laminarin and xylan and therefore constituted candidates for laminarin and xylan hydrolysis.
24727271	4	34	theme	labeled	1049:1055	arg1	laminarin					1057:1065	laminarin	1057:1065	laminarin	1057:1065	Several Verrucomicrobia water column phylotypes were affiliated with previously sequenced, glycoside hydrolase-rich genomes of individual Verrucomicrobia cells that bound fluorescently labeled laminarin and xylan and therefore constituted candidates for laminarin and xylan hydrolysis.
24727271	7	35	theme	changing	1670:1677	arg1	frequency					1689:1697	the changing detection frequency	1666:1697	the changing detection frequency of Verrucomicrobia in the water column	1666:1736	Comparisons with data from the same fjord in the previous summer showed that the bacterial community in Smeerenburgfjord changed in composition, most conspicuously in the changing detection frequency of Verrucomicrobia in the water column.
24727271	7	36	from	frequency	1689:1697	arg1	column					1731:1736	the water column	1721:1736	the water column	1721:1736	Comparisons with data from the same fjord in the previous summer showed that the bacterial community in Smeerenburgfjord changed in composition, most conspicuously in the changing detection frequency of Verrucomicrobia in the water column.
24727271	1	37	theme	pyrotag	284:290	arg1	surveys					292:298	pyrotag surveys	284:298	pyrotag surveys	284:298	In Arctic marine bacterial communities, members of the phylum Verrucomicrobia are consistently detected, although not typically abundant, in 16S rRNA gene clone libraries and pyrotag surveys of the marine water column and in sediments.
24727271	4	38	theme	water	888:892	arg1	phylotypes					901:910	Several Verrucomicrobia water column phylotypes	864:910	Several Verrucomicrobia water column phylotypes	864:910	Several Verrucomicrobia water column phylotypes were affiliated with previously sequenced, glycoside hydrolase-rich genomes of individual Verrucomicrobia cells that bound fluorescently labeled laminarin and xylan and therefore constituted candidates for laminarin and xylan hydrolysis.
24727271	5	39	theme	water	1358:1362	arg1	column					1364:1369	the water column	1354:1369	the water column	1354:1369	In sediments, the bacterial community was dominated by different lineages of Verrucomicrobia, Bacteroidetes, and Proteobacteria but also included members of multiple phylum-level lineages not observed in the water column.
24727271	4	40	theme	Verrucomicrobia	872:886	arg1	phylotypes					901:910	Several Verrucomicrobia water column phylotypes	864:910	Several Verrucomicrobia water column phylotypes	864:910	Several Verrucomicrobia water column phylotypes were affiliated with previously sequenced, glycoside hydrolase-rich genomes of individual Verrucomicrobia cells that bound fluorescently labeled laminarin and xylan and therefore constituted candidates for laminarin and xylan hydrolysis.
24727271	7	41	from	column	1731:1736	arg1	frequency					1689:1697	the changing detection frequency	1666:1697	the changing detection frequency of Verrucomicrobia in the water column	1666:1736	Comparisons with data from the same fjord in the previous summer showed that the bacterial community in Smeerenburgfjord changed in composition, most conspicuously in the changing detection frequency of Verrucomicrobia in the water column.
24727271	7	42	from	fjord	1535:1539	arg1	data					1516:1519	data	1516:1519	data from the same fjord in the previous summer	1516:1562	Comparisons with data from the same fjord in the previous summer showed that the bacterial community in Smeerenburgfjord changed in composition, most conspicuously in the changing detection frequency of Verrucomicrobia in the water column.
24727271	7	42	from	fjord	1535:1539	arg1	summer					1557:1562	the previous summer	1544:1562	the previous summer	1544:1562	Comparisons with data from the same fjord in the previous summer showed that the bacterial community in Smeerenburgfjord changed in composition, most conspicuously in the changing detection frequency of Verrucomicrobia in the water column.
24727271	2	43	theme	community	573:581	arg1	members					583:589	bacterioplankton community members	556:589	bacterioplankton community members	556:589	In an Arctic fjord (Smeerenburgfjord) of Svalbard, members of the Verrucomicrobia, together with Flavobacteria and smaller proportions of Alpha- and Gammaproteobacteria, constituted the most frequently detected bacterioplankton community members in 16S rRNA gene-based clone library analyses of the water column.
24727271	5	44	located	observed	1342:1349	arg1	column					1364:1369	the water column	1354:1369	the water column	1354:1369	In sediments, the bacterial community was dominated by different lineages of Verrucomicrobia, Bacteroidetes, and Proteobacteria but also included members of multiple phylum-level lineages not observed in the water column.
24727271	5	44	located	observed	1342:1349	arg2	lineages					1329:1336	multiple phylum-level lineages	1307:1336	multiple phylum-level lineages not observed in the water column	1307:1369	In sediments, the bacterial community was dominated by different lineages of Verrucomicrobia, Bacteroidetes, and Proteobacteria but also included members of multiple phylum-level lineages not observed in the water column.
24727271	1	45	theme	Arctic	112:117	arg1	communities					136:146	Arctic marine bacterial communities	112:146	Arctic marine bacterial communities	112:146	In Arctic marine bacterial communities, members of the phylum Verrucomicrobia are consistently detected, although not typically abundant, in 16S rRNA gene clone libraries and pyrotag surveys of the marine water column and in sediments.
24727271	2	46	theme	Verrucomicrobia	411:425	arg1	members					396:402	members	396:402	members	396:402	In an Arctic fjord (Smeerenburgfjord) of Svalbard, members of the Verrucomicrobia, together with Flavobacteria and smaller proportions of Alpha- and Gammaproteobacteria, constituted the most frequently detected bacterioplankton community members in 16S rRNA gene-based clone library analyses of the water column.
24727271	2	47	theme	bacterioplankton	556:571	arg1	members					583:589	bacterioplankton community members	556:589	bacterioplankton community members	556:589	In an Arctic fjord (Smeerenburgfjord) of Svalbard, members of the Verrucomicrobia, together with Flavobacteria and smaller proportions of Alpha- and Gammaproteobacteria, constituted the most frequently detected bacterioplankton community members in 16S rRNA gene-based clone library analyses of the water column.
24727271	7	48	from	Verrucomicrobia	1702:1716	arg1	column					1731:1736	the water column	1721:1736	the water column	1721:1736	Comparisons with data from the same fjord in the previous summer showed that the bacterial community in Smeerenburgfjord changed in composition, most conspicuously in the changing detection frequency of Verrucomicrobia in the water column.
24727271	1	49	theme	marine	119:124	arg1	communities					136:146	Arctic marine bacterial communities	112:146	Arctic marine bacterial communities	112:146	In Arctic marine bacterial communities, members of the phylum Verrucomicrobia are consistently detected, although not typically abundant, in 16S rRNA gene clone libraries and pyrotag surveys of the marine water column and in sediments.
24727271	8	50	theme	same	1795:1798	arg1	substrates					1815:1824	the same polysaccharide substrates	1791:1824	the same polysaccharide substrates	1791:1824	Nonetheless, in both years the community hydrolyzed the same polysaccharide substrates.
24727271	6	51	theme	chondroitin	1416:1426	arg1	sulfate					1428:1434	chondroitin sulfate	1416:1434	chondroitin sulfate	1416:1434	This community hydrolyzed laminarin, xylan, chondroitin sulfate, and three additional polysaccharide substrates at high rates.
24727271	3	52	theme	chondroitin	775:785	arg1	sulfate					787:793	chondroitin sulfate	775:793	chondroitin sulfate	775:793	Parallel measurements in the water column of the activities of six endo-acting polysaccharide hydrolases showed that chondroitin sulfate, laminarin, and xylan hydrolysis accounted for most of the activity.
24727271	4	53	theme	individual	991:1000	arg1	cells					1018:1022	individual Verrucomicrobia cells	991:1022	individual Verrucomicrobia cells that bound fluorescently labeled laminarin and xylan and therefore constituted candidates for laminarin and xylan hydrolysis	991:1147	Several Verrucomicrobia water column phylotypes were affiliated with previously sequenced, glycoside hydrolase-rich genomes of individual Verrucomicrobia cells that bound fluorescently labeled laminarin and xylan and therefore constituted candidates for laminarin and xylan hydrolysis.
24727271	0	54	theme	polysaccharide-degrading	35:58	arg1	bacterioplankton					60:75	polysaccharide-degrading bacterioplankton	35:75	polysaccharide-degrading bacterioplankton	35:75	Verrucomicrobia are candidates for polysaccharide-degrading bacterioplankton in an arctic fjord of Svalbard.
24727271	1	55	theme	marine	307:312	arg1	column					320:325	the marine water column	303:325	the marine water column	303:325	In Arctic marine bacterial communities, members of the phylum Verrucomicrobia are consistently detected, although not typically abundant, in 16S rRNA gene clone libraries and pyrotag surveys of the marine water column and in sediments.
24727271	2	56	theme	smaller	460:466	arg1	proportions					468:478	smaller proportions	460:478	smaller proportions of Alpha- and Gammaproteobacteria	460:512	In an Arctic fjord (Smeerenburgfjord) of Svalbard, members of the Verrucomicrobia, together with Flavobacteria and smaller proportions of Alpha- and Gammaproteobacteria, constituted the most frequently detected bacterioplankton community members in 16S rRNA gene-based clone library analyses of the water column.
24727271	7	57	with	Comparisons	1499:1509	arg1	data					1516:1519	data	1516:1519	data from the same fjord in the previous summer	1516:1562	Comparisons with data from the same fjord in the previous summer showed that the bacterial community in Smeerenburgfjord changed in composition, most conspicuously in the changing detection frequency of Verrucomicrobia in the water column.
24727271	7	58	from	community	1590:1598	arg1	Smeerenburgfjord					1603:1618	Smeerenburgfjord	1603:1618	Smeerenburgfjord	1603:1618	Comparisons with data from the same fjord in the previous summer showed that the bacterial community in Smeerenburgfjord changed in composition, most conspicuously in the changing detection frequency of Verrucomicrobia in the water column.
24727271	1	59	theme	water	314:318	arg1	column					320:325	the marine water column	303:325	the marine water column	303:325	In Arctic marine bacterial communities, members of the phylum Verrucomicrobia are consistently detected, although not typically abundant, in 16S rRNA gene clone libraries and pyrotag surveys of the marine water column and in sediments.
24727271	2	60	theme	rRNA	598:601	arg1	library					620:626	16S rRNA gene-based clone library	594:626	16S rRNA gene-based clone library analyses of the water column	594:655	In an Arctic fjord (Smeerenburgfjord) of Svalbard, members of the Verrucomicrobia, together with Flavobacteria and smaller proportions of Alpha- and Gammaproteobacteria, constituted the most frequently detected bacterioplankton community members in 16S rRNA gene-based clone library analyses of the water column.
24727271	1	61	located	detected	204:211	arg1	libraries					270:278	16S rRNA gene clone libraries	250:278	16S rRNA gene clone libraries	250:278	In Arctic marine bacterial communities, members of the phylum Verrucomicrobia are consistently detected, although not typically abundant, in 16S rRNA gene clone libraries and pyrotag surveys of the marine water column and in sediments.
24727271	1	61	located	detected	204:211	arg2	members					149:155	members	149:155	members of the phylum Verrucomicrobia	149:185	In Arctic marine bacterial communities, members of the phylum Verrucomicrobia are consistently detected, although not typically abundant, in 16S rRNA gene clone libraries and pyrotag surveys of the marine water column and in sediments.
24727271	1	61	located	detected	204:211	arg1	communities					136:146	Arctic marine bacterial communities	112:146	Arctic marine bacterial communities	112:146	In Arctic marine bacterial communities, members of the phylum Verrucomicrobia are consistently detected, although not typically abundant, in 16S rRNA gene clone libraries and pyrotag surveys of the marine water column and in sediments.
24727271	7	62	theme	previous	1548:1555	arg1	summer					1557:1562	the previous summer	1544:1562	the previous summer	1544:1562	Comparisons with data from the same fjord in the previous summer showed that the bacterial community in Smeerenburgfjord changed in composition, most conspicuously in the changing detection frequency of Verrucomicrobia in the water column.
24727271	1	63	theme	bacterial	126:134	arg1	communities					136:146	Arctic marine bacterial communities	112:146	Arctic marine bacterial communities	112:146	In Arctic marine bacterial communities, members of the phylum Verrucomicrobia are consistently detected, although not typically abundant, in 16S rRNA gene clone libraries and pyrotag surveys of the marine water column and in sediments.
24727271	5	64	theme	Verrucomicrobia	1227:1241	arg1	lineages					1215:1222	different lineages	1205:1222	different lineages of Verrucomicrobia, Bacteroidetes, and Proteobacteria	1205:1276	In sediments, the bacterial community was dominated by different lineages of Verrucomicrobia, Bacteroidetes, and Proteobacteria but also included members of multiple phylum-level lineages not observed in the water column.
24727271	1	65	theme	column	320:325	arg1	libraries					270:278	16S rRNA gene clone libraries	250:278	16S rRNA gene clone libraries	250:278	In Arctic marine bacterial communities, members of the phylum Verrucomicrobia are consistently detected, although not typically abundant, in 16S rRNA gene clone libraries and pyrotag surveys of the marine water column and in sediments.
24727271	2	66	theme	16S	594:596	arg1	library					620:626	16S rRNA gene-based clone library	594:626	16S rRNA gene-based clone library analyses of the water column	594:655	In an Arctic fjord (Smeerenburgfjord) of Svalbard, members of the Verrucomicrobia, together with Flavobacteria and smaller proportions of Alpha- and Gammaproteobacteria, constituted the most frequently detected bacterioplankton community members in 16S rRNA gene-based clone library analyses of the water column.
24727271	3	67	theme	Parallel	658:665	arg1	measurements					667:678	Parallel measurements	658:678	Parallel measurements in the water column of the activities of six endo-acting polysaccharide hydrolases	658:761	Parallel measurements in the water column of the activities of six endo-acting polysaccharide hydrolases showed that chondroitin sulfate, laminarin, and xylan hydrolysis accounted for most of the activity.
24727271	4	68	theme	Several	864:870	arg1	phylotypes					901:910	Several Verrucomicrobia water column phylotypes	864:910	Several Verrucomicrobia water column phylotypes	864:910	Several Verrucomicrobia water column phylotypes were affiliated with previously sequenced, glycoside hydrolase-rich genomes of individual Verrucomicrobia cells that bound fluorescently labeled laminarin and xylan and therefore constituted candidates for laminarin and xylan hydrolysis.
24727271	4	69	theme	glycoside	955:963	arg1	genomes					980:986	previously sequenced, glycoside hydrolase-rich genomes	933:986	genomes	980:986	Several Verrucomicrobia water column phylotypes were affiliated with previously sequenced, glycoside hydrolase-rich genomes of individual Verrucomicrobia cells that bound fluorescently labeled laminarin and xylan and therefore constituted candidates for laminarin and xylan hydrolysis.
24727271	7	70	theme	bacterial	1580:1588	arg1	community					1590:1598	the bacterial community	1576:1598	the bacterial community in Smeerenburgfjord	1576:1618	Comparisons with data from the same fjord in the previous summer showed that the bacterial community in Smeerenburgfjord changed in composition, most conspicuously in the changing detection frequency of Verrucomicrobia in the water column.
24727271	5	71	theme	lineages	1329:1336	arg1	members					1296:1302	members	1296:1302	members of multiple phylum-level lineages not observed in the water column	1296:1369	In sediments, the bacterial community was dominated by different lineages of Verrucomicrobia, Bacteroidetes, and Proteobacteria but also included members of multiple phylum-level lineages not observed in the water column.
24727271	6	72	theme	high	1487:1490	arg1	rates					1492:1496	high rates	1487:1496	high rates	1487:1496	This community hydrolyzed laminarin, xylan, chondroitin sulfate, and three additional polysaccharide substrates at high rates.
24727271	4	73	dep	laminarin	1118:1126	arg1	hydrolysis					1138:1147	hydrolysis	1138:1147	hydrolysis	1138:1147	Several Verrucomicrobia water column phylotypes were affiliated with previously sequenced, glycoside hydrolase-rich genomes of individual Verrucomicrobia cells that bound fluorescently labeled laminarin and xylan and therefore constituted candidates for laminarin and xylan hydrolysis.
24727271	2	74	theme	Gammaproteobacteria	494:512	arg1	Flavobacteria					442:454	Flavobacteria	442:454	Flavobacteria	442:454	In an Arctic fjord (Smeerenburgfjord) of Svalbard, members of the Verrucomicrobia, together with Flavobacteria and smaller proportions of Alpha- and Gammaproteobacteria, constituted the most frequently detected bacterioplankton community members in 16S rRNA gene-based clone library analyses of the water column.
24727271	2	74	theme	Gammaproteobacteria	494:512	arg1	proportions					468:478	smaller proportions	460:478	smaller proportions of Alpha- and Gammaproteobacteria	460:512	In an Arctic fjord (Smeerenburgfjord) of Svalbard, members of the Verrucomicrobia, together with Flavobacteria and smaller proportions of Alpha- and Gammaproteobacteria, constituted the most frequently detected bacterioplankton community members in 16S rRNA gene-based clone library analyses of the water column.
24727271	3	75	theme	water	687:691	arg1	column					693:698	the water column	683:698	the water column of the activities of six endo-acting polysaccharide hydrolases	683:761	Parallel measurements in the water column of the activities of six endo-acting polysaccharide hydrolases showed that chondroitin sulfate, laminarin, and xylan hydrolysis accounted for most of the activity.
24727271	5	76	theme	different	1205:1213	arg1	lineages					1215:1222	different lineages	1205:1222	different lineages of Verrucomicrobia, Bacteroidetes, and Proteobacteria	1205:1276	In sediments, the bacterial community was dominated by different lineages of Verrucomicrobia, Bacteroidetes, and Proteobacteria but also included members of multiple phylum-level lineages not observed in the water column.
24727271	5	77	theme	Proteobacteria	1263:1276	arg1	lineages					1215:1222	different lineages	1205:1222	different lineages of Verrucomicrobia, Bacteroidetes, and Proteobacteria	1205:1276	In sediments, the bacterial community was dominated by different lineages of Verrucomicrobia, Bacteroidetes, and Proteobacteria but also included members of multiple phylum-level lineages not observed in the water column.
24727271	1	78	theme	16S	250:252	arg1	libraries					270:278	16S rRNA gene clone libraries	250:278	16S rRNA gene clone libraries	250:278	In Arctic marine bacterial communities, members of the phylum Verrucomicrobia are consistently detected, although not typically abundant, in 16S rRNA gene clone libraries and pyrotag surveys of the marine water column and in sediments.
24727271	0	79	theme	arctic	83:88	arg1	fjord					90:94	an arctic fjord	80:94	an arctic fjord of Svalbard	80:106	Verrucomicrobia are candidates for polysaccharide-degrading bacterioplankton in an arctic fjord of Svalbard.
24727271	5	80	theme	Bacteroidetes	1244:1256	arg1	lineages					1215:1222	different lineages	1205:1222	different lineages of Verrucomicrobia, Bacteroidetes, and Proteobacteria	1205:1276	In sediments, the bacterial community was dominated by different lineages of Verrucomicrobia, Bacteroidetes, and Proteobacteria but also included members of multiple phylum-level lineages not observed in the water column.
24727271	7	81	theme	Verrucomicrobia	1702:1716	arg1	frequency					1689:1697	the changing detection frequency	1666:1697	the changing detection frequency of Verrucomicrobia in the water column	1666:1736	Comparisons with data from the same fjord in the previous summer showed that the bacterial community in Smeerenburgfjord changed in composition, most conspicuously in the changing detection frequency of Verrucomicrobia in the water column.
24727271	8	82	theme	polysaccharide	1800:1813	arg1	substrates					1815:1824	the same polysaccharide substrates	1791:1824	the same polysaccharide substrates	1791:1824	Nonetheless, in both years the community hydrolyzed the same polysaccharide substrates.
24727271	1	83	theme	rRNA	254:257	arg1	libraries					270:278	16S rRNA gene clone libraries	250:278	16S rRNA gene clone libraries	250:278	In Arctic marine bacterial communities, members of the phylum Verrucomicrobia are consistently detected, although not typically abundant, in 16S rRNA gene clone libraries and pyrotag surveys of the marine water column and in sediments.
26331907	3	0	theme	molecular	584:592	arg1	factors					603:609	molecular response factors	584:609	molecular response factors	584:609	Despite the widespread use of electrospray ionization for metabolite analyses, the fundamentals needed for reliable prediction of molecular response factors have yet to be explored in detail for analytes that lack ionized functional groups.
26331907	1	1	theme	chromatography/mass	313:331	arg1	LC/MS					347:351	LC/MS	347:351	LC/MS	347:351	Authentic standards of known concentrations serve as references for accurate absolute quantification of plant metabolites using liquid chromatography/mass spectrometry (LC/MS).
26331907	1	1	theme	chromatography/mass	313:331	arg1	spectrometry					333:344	liquid chromatography/mass spectrometry	306:344	liquid chromatography/mass spectrometry (LC/MS)	306:352	Authentic standards of known concentrations serve as references for accurate absolute quantification of plant metabolites using liquid chromatography/mass spectrometry (LC/MS).
26331907	4	2	theme	dilution	996:1003	arg1	spectrometry					1061:1072	stable-isotope dilution ultrahigh performance liquid chromatography-tandem mass spectrometry	981:1072	stable-isotope dilution ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS)	981:1086	In order to lay a foundation for quantifying unknown neutral plant metabolites in absence of authentic standards, sub-milligram quantities of purified homologous acylsucrose metabolites were authenticated by subjecting each to basic hydrolysis and quantifying the sucrose product using stable-isotope dilution ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) with multiple reaction monitoring (MRM).
26331907	4	2	theme	dilution	996:1003	arg1	UHPLC-MS/MS					1075:1085	UHPLC-MS/MS	1075:1085	UHPLC-MS/MS	1075:1085	In order to lay a foundation for quantifying unknown neutral plant metabolites in absence of authentic standards, sub-milligram quantities of purified homologous acylsucrose metabolites were authenticated by subjecting each to basic hydrolysis and quantifying the sucrose product using stable-isotope dilution ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) with multiple reaction monitoring (MRM).
26331907	7	3	from	leaves	1825:1830	arg1	extract					1812:1818	an extract	1809:1818	an extract from leaves of the wild tomato Solanum habrochaites LA1777	1809:1877	Absolute UHPLC-MS quantification was performed on these three metabolites in an extract from leaves of the wild tomato Solanum habrochaites LA1777, yielding deviations of 26%, 6.7%, and 7.3% from values established using compound-specific response factors.
26331907	7	4	theme	%	1905:1905	arg1	deviations					1889:1898	deviations	1889:1898	deviations of 26%, 6.7%, and 7.3% from values established using compound-specific response factors	1889:1986	Absolute UHPLC-MS quantification was performed on these three metabolites in an extract from leaves of the wild tomato Solanum habrochaites LA1777, yielding deviations of 26%, 6.7%, and 7.3% from values established using compound-specific response factors.
26331907	5	5	theme	%	1303:1303	arg1	acetonitrile					1305:1316	40%-80% acetonitrile	1297:1316	40%-80% acetonitrile	1297:1316	Once authenticated, molar response factors of [M + formate](-) ions for the acylsucrose metabolites were determined at different mobile phase compositions ranging from 40%-80% acetonitrile, and demonstrated relationships of response factors with mobile phase composition and metabolite structural features including nonpolar surface areas, the length of the longest acyl chain, and the number of hydroxyl groups.
26331907	4	6	theme	sucrose	959:965	arg1	product					967:973	the sucrose product	955:973	the sucrose product using stable-isotope dilution ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS)	955:1086	In order to lay a foundation for quantifying unknown neutral plant metabolites in absence of authentic standards, sub-milligram quantities of purified homologous acylsucrose metabolites were authenticated by subjecting each to basic hydrolysis and quantifying the sucrose product using stable-isotope dilution ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) with multiple reaction monitoring (MRM).
26331907	5	7	dep	phase	1382:1386	arg1	composition					1388:1398	composition	1388:1398	composition	1388:1398	Once authenticated, molar response factors of [M + formate](-) ions for the acylsucrose metabolites were determined at different mobile phase compositions ranging from 40%-80% acetonitrile, and demonstrated relationships of response factors with mobile phase composition and metabolite structural features including nonpolar surface areas, the length of the longest acyl chain, and the number of hydroxyl groups.
26331907	4	8	theme	unknown	740:746	arg1	metabolites					762:772	unknown neutral plant metabolites	740:772	unknown neutral plant metabolites	740:772	In order to lay a foundation for quantifying unknown neutral plant metabolites in absence of authentic standards, sub-milligram quantities of purified homologous acylsucrose metabolites were authenticated by subjecting each to basic hydrolysis and quantifying the sucrose product using stable-isotope dilution ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) with multiple reaction monitoring (MRM).
26331907	5	9	theme	hydroxyl	1525:1532	arg1	groups					1534:1539	hydroxyl groups	1525:1539	hydroxyl groups	1525:1539	Once authenticated, molar response factors of [M + formate](-) ions for the acylsucrose metabolites were determined at different mobile phase compositions ranging from 40%-80% acetonitrile, and demonstrated relationships of response factors with mobile phase composition and metabolite structural features including nonpolar surface areas, the length of the longest acyl chain, and the number of hydroxyl groups.
26331907	2	10	theme	such	370:373	arg1	standards					375:383	often such standards	364:383	often such standards	364:383	However, often such standards are not commercially available or not amenable for custom syntheses.
26331907	0	11	theme	neutral	145:151	arg1	metabolites					165:175	newly-authenticated neutral acylsucrose metabolites	125:175	newly-authenticated neutral acylsucrose metabolites	125:175	Dependence of negative-mode electrospray ionization response factors on mobile phase composition and molecular structure for newly-authenticated neutral acylsucrose metabolites.
26331907	1	12	theme	accurate	246:253	arg1	quantification					264:277	accurate absolute quantification	246:277	accurate absolute quantification of plant metabolites using liquid chromatography/mass spectrometry (LC/MS)	246:352	Authentic standards of known concentrations serve as references for accurate absolute quantification of plant metabolites using liquid chromatography/mass spectrometry (LC/MS).
26331907	3	13	theme	ionization	497:506	arg1	use					477:479	the widespread use	462:479	the widespread use of electrospray ionization for metabolite analyses	462:530	Despite the widespread use of electrospray ionization for metabolite analyses, the fundamentals needed for reliable prediction of molecular response factors have yet to be explored in detail for analytes that lack ionized functional groups.
26331907	3	14	theme	ionized	668:674	arg1	groups					687:692	ionized functional groups	668:692	ionized functional groups	668:692	Despite the widespread use of electrospray ionization for metabolite analyses, the fundamentals needed for reliable prediction of molecular response factors have yet to be explored in detail for analytes that lack ionized functional groups.
26331907	6	15	theme	acyl	1713:1716	arg1	atoms					1725:1729	total acyl carbon atoms	1707:1729	total acyl carbon atoms	1707:1729	This approach was employed to calculate predicted response factors for three authenticated acylsucroses based on mean values for all isomers with a common number of total acyl carbon atoms.
26331907	7	16	theme	Absolute	1732:1739	arg1	quantification					1750:1763	Absolute UHPLC-MS quantification	1732:1763	Absolute UHPLC-MS quantification	1732:1763	Absolute UHPLC-MS quantification was performed on these three metabolites in an extract from leaves of the wild tomato Solanum habrochaites LA1777, yielding deviations of 26%, 6.7%, and 7.3% from values established using compound-specific response factors.
26331907	4	17	theme	performance	1015:1025	arg1	spectrometry					1061:1072	stable-isotope dilution ultrahigh performance liquid chromatography-tandem mass spectrometry	981:1072	stable-isotope dilution ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS)	981:1086	In order to lay a foundation for quantifying unknown neutral plant metabolites in absence of authentic standards, sub-milligram quantities of purified homologous acylsucrose metabolites were authenticated by subjecting each to basic hydrolysis and quantifying the sucrose product using stable-isotope dilution ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) with multiple reaction monitoring (MRM).
26331907	4	17	theme	performance	1015:1025	arg1	UHPLC-MS/MS					1075:1085	UHPLC-MS/MS	1075:1085	UHPLC-MS/MS	1075:1085	In order to lay a foundation for quantifying unknown neutral plant metabolites in absence of authentic standards, sub-milligram quantities of purified homologous acylsucrose metabolites were authenticated by subjecting each to basic hydrolysis and quantifying the sucrose product using stable-isotope dilution ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) with multiple reaction monitoring (MRM).
26331907	5	18	theme	[M	1175:1176	arg1	ions					1192:1195	[M + formate](-) ions	1175:1195	[M + formate](-) ions	1175:1195	Once authenticated, molar response factors of [M + formate](-) ions for the acylsucrose metabolites were determined at different mobile phase compositions ranging from 40%-80% acetonitrile, and demonstrated relationships of response factors with mobile phase composition and metabolite structural features including nonpolar surface areas, the length of the longest acyl chain, and the number of hydroxyl groups.
26331907	7	19	theme	habrochaites	1859:1870	arg1	LA1777					1872:1877	the wild tomato Solanum habrochaites LA1777	1835:1877	the wild tomato Solanum habrochaites LA1777	1835:1877	Absolute UHPLC-MS quantification was performed on these three metabolites in an extract from leaves of the wild tomato Solanum habrochaites LA1777, yielding deviations of 26%, 6.7%, and 7.3% from values established using compound-specific response factors.
26331907	4	20	theme	chromatography-tandem	1034:1054	arg1	spectrometry					1061:1072	stable-isotope dilution ultrahigh performance liquid chromatography-tandem mass spectrometry	981:1072	stable-isotope dilution ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS)	981:1086	In order to lay a foundation for quantifying unknown neutral plant metabolites in absence of authentic standards, sub-milligram quantities of purified homologous acylsucrose metabolites were authenticated by subjecting each to basic hydrolysis and quantifying the sucrose product using stable-isotope dilution ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) with multiple reaction monitoring (MRM).
26331907	4	20	theme	chromatography-tandem	1034:1054	arg1	UHPLC-MS/MS					1075:1085	UHPLC-MS/MS	1075:1085	UHPLC-MS/MS	1075:1085	In order to lay a foundation for quantifying unknown neutral plant metabolites in absence of authentic standards, sub-milligram quantities of purified homologous acylsucrose metabolites were authenticated by subjecting each to basic hydrolysis and quantifying the sucrose product using stable-isotope dilution ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) with multiple reaction monitoring (MRM).
26331907	4	21	theme	standards	798:806	arg1	absence					777:783	absence	777:783	absence of authentic standards	777:806	In order to lay a foundation for quantifying unknown neutral plant metabolites in absence of authentic standards, sub-milligram quantities of purified homologous acylsucrose metabolites were authenticated by subjecting each to basic hydrolysis and quantifying the sucrose product using stable-isotope dilution ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) with multiple reaction monitoring (MRM).
26331907	5	22	theme	formate	1180:1186	arg1	ions					1192:1195	[M + formate](-) ions	1175:1195	[M + formate](-) ions	1175:1195	Once authenticated, molar response factors of [M + formate](-) ions for the acylsucrose metabolites were determined at different mobile phase compositions ranging from 40%-80% acetonitrile, and demonstrated relationships of response factors with mobile phase composition and metabolite structural features including nonpolar surface areas, the length of the longest acyl chain, and the number of hydroxyl groups.
26331907	6	23	theme	common	1690:1695	arg1	number					1697:1702	a common number	1688:1702	a common number of total acyl carbon atoms	1688:1729	This approach was employed to calculate predicted response factors for three authenticated acylsucroses based on mean values for all isomers with a common number of total acyl carbon atoms.
26331907	7	24	theme	tomato	1844:1849	arg1	LA1777					1872:1877	the wild tomato Solanum habrochaites LA1777	1835:1877	the wild tomato Solanum habrochaites LA1777	1835:1877	Absolute UHPLC-MS quantification was performed on these three metabolites in an extract from leaves of the wild tomato Solanum habrochaites LA1777, yielding deviations of 26%, 6.7%, and 7.3% from values established using compound-specific response factors.
26331907	4	25	theme	plant	756:760	arg1	metabolites					762:772	unknown neutral plant metabolites	740:772	unknown neutral plant metabolites	740:772	In order to lay a foundation for quantifying unknown neutral plant metabolites in absence of authentic standards, sub-milligram quantities of purified homologous acylsucrose metabolites were authenticated by subjecting each to basic hydrolysis and quantifying the sucrose product using stable-isotope dilution ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) with multiple reaction monitoring (MRM).
26331907	5	26	theme	structural	1415:1424	arg1	features					1426:1433	metabolite structural features	1404:1433	metabolite structural features including nonpolar surface areas, the length of the longest acyl chain, and the number of hydroxyl groups	1404:1539	Once authenticated, molar response factors of [M + formate](-) ions for the acylsucrose metabolites were determined at different mobile phase compositions ranging from 40%-80% acetonitrile, and demonstrated relationships of response factors with mobile phase composition and metabolite structural features including nonpolar surface areas, the length of the longest acyl chain, and the number of hydroxyl groups.
26331907	5	26	theme	structural	1415:1424	arg1	number					1515:1520	the number	1511:1520	the number of hydroxyl groups	1511:1539	Once authenticated, molar response factors of [M + formate](-) ions for the acylsucrose metabolites were determined at different mobile phase compositions ranging from 40%-80% acetonitrile, and demonstrated relationships of response factors with mobile phase composition and metabolite structural features including nonpolar surface areas, the length of the longest acyl chain, and the number of hydroxyl groups.
26331907	5	26	theme	structural	1415:1424	arg1	areas					1462:1466	nonpolar surface areas	1445:1466	nonpolar surface areas	1445:1466	Once authenticated, molar response factors of [M + formate](-) ions for the acylsucrose metabolites were determined at different mobile phase compositions ranging from 40%-80% acetonitrile, and demonstrated relationships of response factors with mobile phase composition and metabolite structural features including nonpolar surface areas, the length of the longest acyl chain, and the number of hydroxyl groups.
26331907	5	26	theme	structural	1415:1424	arg1	length					1473:1478	the length	1469:1478	the length of the longest acyl chain	1469:1504	Once authenticated, molar response factors of [M + formate](-) ions for the acylsucrose metabolites were determined at different mobile phase compositions ranging from 40%-80% acetonitrile, and demonstrated relationships of response factors with mobile phase composition and metabolite structural features including nonpolar surface areas, the length of the longest acyl chain, and the number of hydroxyl groups.
26331907	0	27	theme	mobile	72:77	arg1	composition					85:95	mobile phase composition	72:95	mobile phase composition	72:95	Dependence of negative-mode electrospray ionization response factors on mobile phase composition and molecular structure for newly-authenticated neutral acylsucrose metabolites.
26331907	1	28	theme	known	201:205	arg1	concentrations					207:220	known concentrations	201:220	known concentrations	201:220	Authentic standards of known concentrations serve as references for accurate absolute quantification of plant metabolites using liquid chromatography/mass spectrometry (LC/MS).
26331907	0	29	from	Dependence	0:9	arg1	composition					85:95	mobile phase composition	72:95	mobile phase composition	72:95	Dependence of negative-mode electrospray ionization response factors on mobile phase composition and molecular structure for newly-authenticated neutral acylsucrose metabolites.
26331907	0	29	from	Dependence	0:9	arg1	structure					111:119	molecular structure	101:119	molecular structure	101:119	Dependence of negative-mode electrospray ionization response factors on mobile phase composition and molecular structure for newly-authenticated neutral acylsucrose metabolites.
26331907	4	30	theme	homologous	846:855	arg1	metabolites					869:879	purified homologous acylsucrose metabolites	837:879	purified homologous acylsucrose metabolites	837:879	In order to lay a foundation for quantifying unknown neutral plant metabolites in absence of authentic standards, sub-milligram quantities of purified homologous acylsucrose metabolites were authenticated by subjecting each to basic hydrolysis and quantifying the sucrose product using stable-isotope dilution ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) with multiple reaction monitoring (MRM).
26331907	0	31	theme	molecular	101:109	arg1	structure					111:119	molecular structure	101:119	molecular structure	101:119	Dependence of negative-mode electrospray ionization response factors on mobile phase composition and molecular structure for newly-authenticated neutral acylsucrose metabolites.
26331907	4	32	theme	reaction	1102:1109	arg1	MRM					1123:1125	MRM	1123:1125	MRM	1123:1125	In order to lay a foundation for quantifying unknown neutral plant metabolites in absence of authentic standards, sub-milligram quantities of purified homologous acylsucrose metabolites were authenticated by subjecting each to basic hydrolysis and quantifying the sucrose product using stable-isotope dilution ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) with multiple reaction monitoring (MRM).
26331907	4	32	theme	reaction	1102:1109	arg1	monitoring					1111:1120	multiple reaction monitoring	1093:1120	multiple reaction monitoring (MRM)	1093:1126	In order to lay a foundation for quantifying unknown neutral plant metabolites in absence of authentic standards, sub-milligram quantities of purified homologous acylsucrose metabolites were authenticated by subjecting each to basic hydrolysis and quantifying the sucrose product using stable-isotope dilution ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) with multiple reaction monitoring (MRM).
26331907	4	33	theme	metabolites	869:879	arg1	quantities					823:832	sub-milligram quantities	809:832	sub-milligram quantities of purified homologous acylsucrose metabolites	809:879	In order to lay a foundation for quantifying unknown neutral plant metabolites in absence of authentic standards, sub-milligram quantities of purified homologous acylsucrose metabolites were authenticated by subjecting each to basic hydrolysis and quantifying the sucrose product using stable-isotope dilution ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) with multiple reaction monitoring (MRM).
26331907	5	34	theme	acylsucrose	1205:1215	arg1	metabolites					1217:1227	the acylsucrose metabolites	1201:1227	the acylsucrose metabolites	1201:1227	Once authenticated, molar response factors of [M + formate](-) ions for the acylsucrose metabolites were determined at different mobile phase compositions ranging from 40%-80% acetonitrile, and demonstrated relationships of response factors with mobile phase composition and metabolite structural features including nonpolar surface areas, the length of the longest acyl chain, and the number of hydroxyl groups.
26331907	4	35	theme	sub-milligram	809:821	arg1	quantities					823:832	sub-milligram quantities	809:832	sub-milligram quantities of purified homologous acylsucrose metabolites	809:879	In order to lay a foundation for quantifying unknown neutral plant metabolites in absence of authentic standards, sub-milligram quantities of purified homologous acylsucrose metabolites were authenticated by subjecting each to basic hydrolysis and quantifying the sucrose product using stable-isotope dilution ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) with multiple reaction monitoring (MRM).
26331907	3	36	theme	electrospray	484:495	arg1	ionization					497:506	electrospray ionization	484:506	electrospray ionization	484:506	Despite the widespread use of electrospray ionization for metabolite analyses, the fundamentals needed for reliable prediction of molecular response factors have yet to be explored in detail for analytes that lack ionized functional groups.
26331907	6	37	theme	atoms	1725:1729	arg1	number					1697:1702	a common number	1688:1702	a common number of total acyl carbon atoms	1688:1729	This approach was employed to calculate predicted response factors for three authenticated acylsucroses based on mean values for all isomers with a common number of total acyl carbon atoms.
26331907	0	38	theme	negative-mode	14:26	arg1	factors					61:67	negative-mode electrospray ionization response factors	14:67	negative-mode electrospray ionization response factors	14:67	Dependence of negative-mode electrospray ionization response factors on mobile phase composition and molecular structure for newly-authenticated neutral acylsucrose metabolites.
26331907	5	39	theme	%	1299:1299	arg1	acetonitrile					1305:1316	40%-80% acetonitrile	1297:1316	40%-80% acetonitrile	1297:1316	Once authenticated, molar response factors of [M + formate](-) ions for the acylsucrose metabolites were determined at different mobile phase compositions ranging from 40%-80% acetonitrile, and demonstrated relationships of response factors with mobile phase composition and metabolite structural features including nonpolar surface areas, the length of the longest acyl chain, and the number of hydroxyl groups.
26331907	5	40	theme	surface	1454:1460	arg1	areas					1462:1466	nonpolar surface areas	1445:1466	nonpolar surface areas	1445:1466	Once authenticated, molar response factors of [M + formate](-) ions for the acylsucrose metabolites were determined at different mobile phase compositions ranging from 40%-80% acetonitrile, and demonstrated relationships of response factors with mobile phase composition and metabolite structural features including nonpolar surface areas, the length of the longest acyl chain, and the number of hydroxyl groups.
26331907	0	41	theme	ionization	41:50	arg1	factors					61:67	negative-mode electrospray ionization response factors	14:67	negative-mode electrospray ionization response factors	14:67	Dependence of negative-mode electrospray ionization response factors on mobile phase composition and molecular structure for newly-authenticated neutral acylsucrose metabolites.
26331907	5	42	theme	mobile	1258:1263	arg1	compositions					1271:1282	different mobile phase compositions	1248:1282	different mobile phase compositions ranging from 40%-80% acetonitrile	1248:1316	Once authenticated, molar response factors of [M + formate](-) ions for the acylsucrose metabolites were determined at different mobile phase compositions ranging from 40%-80% acetonitrile, and demonstrated relationships of response factors with mobile phase composition and metabolite structural features including nonpolar surface areas, the length of the longest acyl chain, and the number of hydroxyl groups.
26331907	5	43	theme	response	1155:1162	arg1	factors					1164:1170	molar response factors	1149:1170	molar response factors of [M + formate](-) ions for the acylsucrose metabolites	1149:1227	Once authenticated, molar response factors of [M + formate](-) ions for the acylsucrose metabolites were determined at different mobile phase compositions ranging from 40%-80% acetonitrile, and demonstrated relationships of response factors with mobile phase composition and metabolite structural features including nonpolar surface areas, the length of the longest acyl chain, and the number of hydroxyl groups.
26331907	3	44	theme	reliable	561:568	arg1	prediction					570:579	reliable prediction	561:579	reliable prediction of molecular response factors	561:609	Despite the widespread use of electrospray ionization for metabolite analyses, the fundamentals needed for reliable prediction of molecular response factors have yet to be explored in detail for analytes that lack ionized functional groups.
26331907	0	45	theme	factors	61:67	arg1	Dependence					0:9	Dependence	0:9	Dependence of negative-mode electrospray ionization response factors on mobile phase composition and molecular structure for newly-authenticated neutral acylsucrose metabolites.	0:176	Dependence of negative-mode electrospray ionization response factors on mobile phase composition and molecular structure for newly-authenticated neutral acylsucrose metabolites.
26331907	7	46	theme	%	1921:1921	arg1	deviations					1889:1898	deviations	1889:1898	deviations of 26%, 6.7%, and 7.3% from values established using compound-specific response factors	1889:1986	Absolute UHPLC-MS quantification was performed on these three metabolites in an extract from leaves of the wild tomato Solanum habrochaites LA1777, yielding deviations of 26%, 6.7%, and 7.3% from values established using compound-specific response factors.
26331907	7	47	theme	compound-specific	1953:1969	arg1	factors					1980:1986	compound-specific response factors	1953:1986	compound-specific response factors	1953:1986	Absolute UHPLC-MS quantification was performed on these three metabolites in an extract from leaves of the wild tomato Solanum habrochaites LA1777, yielding deviations of 26%, 6.7%, and 7.3% from values established using compound-specific response factors.
26331907	3	48	theme	response	594:601	arg1	factors					603:609	molecular response factors	584:609	molecular response factors	584:609	Despite the widespread use of electrospray ionization for metabolite analyses, the fundamentals needed for reliable prediction of molecular response factors have yet to be explored in detail for analytes that lack ionized functional groups.
26331907	6	49	theme	response	1592:1599	arg1	factors					1601:1607	predicted response factors	1582:1607	predicted response factors for three authenticated acylsucroses based on mean values for all isomers with a common number of total acyl carbon atoms	1582:1729	This approach was employed to calculate predicted response factors for three authenticated acylsucroses based on mean values for all isomers with a common number of total acyl carbon atoms.
26331907	1	50	theme	metabolites	288:298	arg1	quantification					264:277	accurate absolute quantification	246:277	accurate absolute quantification of plant metabolites using liquid chromatography/mass spectrometry (LC/MS)	246:352	Authentic standards of known concentrations serve as references for accurate absolute quantification of plant metabolites using liquid chromatography/mass spectrometry (LC/MS).
26331907	7	51	theme	%	1911:1911	arg1	deviations					1889:1898	deviations	1889:1898	deviations of 26%, 6.7%, and 7.3% from values established using compound-specific response factors	1889:1986	Absolute UHPLC-MS quantification was performed on these three metabolites in an extract from leaves of the wild tomato Solanum habrochaites LA1777, yielding deviations of 26%, 6.7%, and 7.3% from values established using compound-specific response factors.
26331907	5	52	theme	acyl	1495:1498	arg1	chain					1500:1504	the longest acyl chain	1483:1504	the longest acyl chain	1483:1504	Once authenticated, molar response factors of [M + formate](-) ions for the acylsucrose metabolites were determined at different mobile phase compositions ranging from 40%-80% acetonitrile, and demonstrated relationships of response factors with mobile phase composition and metabolite structural features including nonpolar surface areas, the length of the longest acyl chain, and the number of hydroxyl groups.
26331907	1	53	theme	liquid	306:311	arg1	LC/MS					347:351	LC/MS	347:351	LC/MS	347:351	Authentic standards of known concentrations serve as references for accurate absolute quantification of plant metabolites using liquid chromatography/mass spectrometry (LC/MS).
26331907	1	53	theme	liquid	306:311	arg1	spectrometry					333:344	liquid chromatography/mass spectrometry	306:344	liquid chromatography/mass spectrometry (LC/MS)	306:352	Authentic standards of known concentrations serve as references for accurate absolute quantification of plant metabolites using liquid chromatography/mass spectrometry (LC/MS).
26331907	4	54	theme	stable-isotope	981:994	arg1	spectrometry					1061:1072	stable-isotope dilution ultrahigh performance liquid chromatography-tandem mass spectrometry	981:1072	stable-isotope dilution ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS)	981:1086	In order to lay a foundation for quantifying unknown neutral plant metabolites in absence of authentic standards, sub-milligram quantities of purified homologous acylsucrose metabolites were authenticated by subjecting each to basic hydrolysis and quantifying the sucrose product using stable-isotope dilution ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) with multiple reaction monitoring (MRM).
26331907	4	54	theme	stable-isotope	981:994	arg1	UHPLC-MS/MS					1075:1085	UHPLC-MS/MS	1075:1085	UHPLC-MS/MS	1075:1085	In order to lay a foundation for quantifying unknown neutral plant metabolites in absence of authentic standards, sub-milligram quantities of purified homologous acylsucrose metabolites were authenticated by subjecting each to basic hydrolysis and quantifying the sucrose product using stable-isotope dilution ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) with multiple reaction monitoring (MRM).
26331907	1	55	theme	concentrations	207:220	arg1	standards					188:196	Authentic standards	178:196	Authentic standards of known concentrations	178:220	Authentic standards of known concentrations serve as references for accurate absolute quantification of plant metabolites using liquid chromatography/mass spectrometry (LC/MS).
26331907	1	55	theme	concentrations	207:220	arg1	references					231:240	references	231:240	references for accurate absolute quantification of plant metabolites using liquid chromatography/mass spectrometry (LC/MS)	231:352	Authentic standards of known concentrations serve as references for accurate absolute quantification of plant metabolites using liquid chromatography/mass spectrometry (LC/MS).
26331907	6	56	theme	mean	1655:1658	arg1	values					1660:1665	mean values	1655:1665	mean values for all isomers with a common number of total acyl carbon atoms	1655:1729	This approach was employed to calculate predicted response factors for three authenticated acylsucroses based on mean values for all isomers with a common number of total acyl carbon atoms.
26331907	0	57	theme	acylsucrose	153:163	arg1	metabolites					165:175	newly-authenticated neutral acylsucrose metabolites	125:175	newly-authenticated neutral acylsucrose metabolites	125:175	Dependence of negative-mode electrospray ionization response factors on mobile phase composition and molecular structure for newly-authenticated neutral acylsucrose metabolites.
26331907	5	58	theme	groups	1534:1539	arg1	number					1515:1520	the number	1511:1520	the number of hydroxyl groups	1511:1539	Once authenticated, molar response factors of [M + formate](-) ions for the acylsucrose metabolites were determined at different mobile phase compositions ranging from 40%-80% acetonitrile, and demonstrated relationships of response factors with mobile phase composition and metabolite structural features including nonpolar surface areas, the length of the longest acyl chain, and the number of hydroxyl groups.
26331907	5	58	theme	groups	1534:1539	arg1	areas					1462:1466	nonpolar surface areas	1445:1466	nonpolar surface areas	1445:1466	Once authenticated, molar response factors of [M + formate](-) ions for the acylsucrose metabolites were determined at different mobile phase compositions ranging from 40%-80% acetonitrile, and demonstrated relationships of response factors with mobile phase composition and metabolite structural features including nonpolar surface areas, the length of the longest acyl chain, and the number of hydroxyl groups.
26331907	5	58	theme	groups	1534:1539	arg1	length					1473:1478	the length	1469:1478	the length of the longest acyl chain	1469:1504	Once authenticated, molar response factors of [M + formate](-) ions for the acylsucrose metabolites were determined at different mobile phase compositions ranging from 40%-80% acetonitrile, and demonstrated relationships of response factors with mobile phase composition and metabolite structural features including nonpolar surface areas, the length of the longest acyl chain, and the number of hydroxyl groups.
26331907	7	59	theme	UHPLC-MS	1741:1748	arg1	quantification					1750:1763	Absolute UHPLC-MS quantification	1732:1763	Absolute UHPLC-MS quantification	1732:1763	Absolute UHPLC-MS quantification was performed on these three metabolites in an extract from leaves of the wild tomato Solanum habrochaites LA1777, yielding deviations of 26%, 6.7%, and 7.3% from values established using compound-specific response factors.
26331907	4	60	theme	mass	1056:1059	arg1	spectrometry					1061:1072	stable-isotope dilution ultrahigh performance liquid chromatography-tandem mass spectrometry	981:1072	stable-isotope dilution ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS)	981:1086	In order to lay a foundation for quantifying unknown neutral plant metabolites in absence of authentic standards, sub-milligram quantities of purified homologous acylsucrose metabolites were authenticated by subjecting each to basic hydrolysis and quantifying the sucrose product using stable-isotope dilution ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) with multiple reaction monitoring (MRM).
26331907	4	60	theme	mass	1056:1059	arg1	UHPLC-MS/MS					1075:1085	UHPLC-MS/MS	1075:1085	UHPLC-MS/MS	1075:1085	In order to lay a foundation for quantifying unknown neutral plant metabolites in absence of authentic standards, sub-milligram quantities of purified homologous acylsucrose metabolites were authenticated by subjecting each to basic hydrolysis and quantifying the sucrose product using stable-isotope dilution ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) with multiple reaction monitoring (MRM).
26331907	6	61	theme	authenticated	1619:1631	arg1	acylsucroses					1633:1644	three authenticated acylsucroses	1613:1644	three authenticated acylsucroses based on mean values for all isomers with a common number of total acyl carbon atoms	1613:1729	This approach was employed to calculate predicted response factors for three authenticated acylsucroses based on mean values for all isomers with a common number of total acyl carbon atoms.
26331907	1	62	theme	absolute	255:262	arg1	quantification					264:277	accurate absolute quantification	246:277	accurate absolute quantification of plant metabolites using liquid chromatography/mass spectrometry (LC/MS)	246:352	Authentic standards of known concentrations serve as references for accurate absolute quantification of plant metabolites using liquid chromatography/mass spectrometry (LC/MS).
26331907	5	63	theme	ions	1192:1195	arg1	factors					1164:1170	molar response factors	1149:1170	molar response factors of [M + formate](-) ions for the acylsucrose metabolites	1149:1227	Once authenticated, molar response factors of [M + formate](-) ions for the acylsucrose metabolites were determined at different mobile phase compositions ranging from 40%-80% acetonitrile, and demonstrated relationships of response factors with mobile phase composition and metabolite structural features including nonpolar surface areas, the length of the longest acyl chain, and the number of hydroxyl groups.
26331907	6	64	theme	total	1707:1711	arg1	atoms					1725:1729	total acyl carbon atoms	1707:1729	total acyl carbon atoms	1707:1729	This approach was employed to calculate predicted response factors for three authenticated acylsucroses based on mean values for all isomers with a common number of total acyl carbon atoms.
26331907	4	65	theme	ultrahigh	1005:1013	arg1	spectrometry					1061:1072	stable-isotope dilution ultrahigh performance liquid chromatography-tandem mass spectrometry	981:1072	stable-isotope dilution ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS)	981:1086	In order to lay a foundation for quantifying unknown neutral plant metabolites in absence of authentic standards, sub-milligram quantities of purified homologous acylsucrose metabolites were authenticated by subjecting each to basic hydrolysis and quantifying the sucrose product using stable-isotope dilution ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) with multiple reaction monitoring (MRM).
26331907	4	65	theme	ultrahigh	1005:1013	arg1	UHPLC-MS/MS					1075:1085	UHPLC-MS/MS	1075:1085	UHPLC-MS/MS	1075:1085	In order to lay a foundation for quantifying unknown neutral plant metabolites in absence of authentic standards, sub-milligram quantities of purified homologous acylsucrose metabolites were authenticated by subjecting each to basic hydrolysis and quantifying the sucrose product using stable-isotope dilution ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) with multiple reaction monitoring (MRM).
26331907	4	66	theme	liquid	1027:1032	arg1	spectrometry					1061:1072	stable-isotope dilution ultrahigh performance liquid chromatography-tandem mass spectrometry	981:1072	stable-isotope dilution ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS)	981:1086	In order to lay a foundation for quantifying unknown neutral plant metabolites in absence of authentic standards, sub-milligram quantities of purified homologous acylsucrose metabolites were authenticated by subjecting each to basic hydrolysis and quantifying the sucrose product using stable-isotope dilution ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) with multiple reaction monitoring (MRM).
26331907	4	66	theme	liquid	1027:1032	arg1	UHPLC-MS/MS					1075:1085	UHPLC-MS/MS	1075:1085	UHPLC-MS/MS	1075:1085	In order to lay a foundation for quantifying unknown neutral plant metabolites in absence of authentic standards, sub-milligram quantities of purified homologous acylsucrose metabolites were authenticated by subjecting each to basic hydrolysis and quantifying the sucrose product using stable-isotope dilution ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) with multiple reaction monitoring (MRM).
26331907	4	67	theme	authentic	788:796	arg1	standards					798:806	authentic standards	788:806	authentic standards	788:806	In order to lay a foundation for quantifying unknown neutral plant metabolites in absence of authentic standards, sub-milligram quantities of purified homologous acylsucrose metabolites were authenticated by subjecting each to basic hydrolysis and quantifying the sucrose product using stable-isotope dilution ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) with multiple reaction monitoring (MRM).
26331907	5	68	theme	metabolite	1404:1413	arg1	features					1426:1433	metabolite structural features	1404:1433	metabolite structural features including nonpolar surface areas, the length of the longest acyl chain, and the number of hydroxyl groups	1404:1539	Once authenticated, molar response factors of [M + formate](-) ions for the acylsucrose metabolites were determined at different mobile phase compositions ranging from 40%-80% acetonitrile, and demonstrated relationships of response factors with mobile phase composition and metabolite structural features including nonpolar surface areas, the length of the longest acyl chain, and the number of hydroxyl groups.
26331907	5	68	theme	metabolite	1404:1413	arg1	number					1515:1520	the number	1511:1520	the number of hydroxyl groups	1511:1539	Once authenticated, molar response factors of [M + formate](-) ions for the acylsucrose metabolites were determined at different mobile phase compositions ranging from 40%-80% acetonitrile, and demonstrated relationships of response factors with mobile phase composition and metabolite structural features including nonpolar surface areas, the length of the longest acyl chain, and the number of hydroxyl groups.
26331907	5	68	theme	metabolite	1404:1413	arg1	areas					1462:1466	nonpolar surface areas	1445:1466	nonpolar surface areas	1445:1466	Once authenticated, molar response factors of [M + formate](-) ions for the acylsucrose metabolites were determined at different mobile phase compositions ranging from 40%-80% acetonitrile, and demonstrated relationships of response factors with mobile phase composition and metabolite structural features including nonpolar surface areas, the length of the longest acyl chain, and the number of hydroxyl groups.
26331907	5	68	theme	metabolite	1404:1413	arg1	length					1473:1478	the length	1469:1478	the length of the longest acyl chain	1469:1504	Once authenticated, molar response factors of [M + formate](-) ions for the acylsucrose metabolites were determined at different mobile phase compositions ranging from 40%-80% acetonitrile, and demonstrated relationships of response factors with mobile phase composition and metabolite structural features including nonpolar surface areas, the length of the longest acyl chain, and the number of hydroxyl groups.
26331907	0	69	theme	phase	79:83	arg1	composition					85:95	mobile phase composition	72:95	mobile phase composition	72:95	Dependence of negative-mode electrospray ionization response factors on mobile phase composition and molecular structure for newly-authenticated neutral acylsucrose metabolites.
26331907	3	70	theme	functional	676:685	arg1	groups					687:692	ionized functional groups	668:692	ionized functional groups	668:692	Despite the widespread use of electrospray ionization for metabolite analyses, the fundamentals needed for reliable prediction of molecular response factors have yet to be explored in detail for analytes that lack ionized functional groups.
26331907	1	71	theme	Authentic	178:186	arg1	standards					188:196	Authentic standards	178:196	Authentic standards of known concentrations	178:220	Authentic standards of known concentrations serve as references for accurate absolute quantification of plant metabolites using liquid chromatography/mass spectrometry (LC/MS).
26331907	1	71	theme	Authentic	178:186	arg1	references					231:240	references	231:240	references for accurate absolute quantification of plant metabolites using liquid chromatography/mass spectrometry (LC/MS)	231:352	Authentic standards of known concentrations serve as references for accurate absolute quantification of plant metabolites using liquid chromatography/mass spectrometry (LC/MS).
26331907	7	72	theme	Solanum	1851:1857	arg1	LA1777					1872:1877	the wild tomato Solanum habrochaites LA1777	1835:1877	the wild tomato Solanum habrochaites LA1777	1835:1877	Absolute UHPLC-MS quantification was performed on these three metabolites in an extract from leaves of the wild tomato Solanum habrochaites LA1777, yielding deviations of 26%, 6.7%, and 7.3% from values established using compound-specific response factors.
26331907	4	73	theme	neutral	748:754	arg1	metabolites					762:772	unknown neutral plant metabolites	740:772	unknown neutral plant metabolites	740:772	In order to lay a foundation for quantifying unknown neutral plant metabolites in absence of authentic standards, sub-milligram quantities of purified homologous acylsucrose metabolites were authenticated by subjecting each to basic hydrolysis and quantifying the sucrose product using stable-isotope dilution ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) with multiple reaction monitoring (MRM).
26331907	5	74	theme	factors	1362:1368	arg1	relationships					1336:1348	relationships	1336:1348	relationships of response factors	1336:1368	Once authenticated, molar response factors of [M + formate](-) ions for the acylsucrose metabolites were determined at different mobile phase compositions ranging from 40%-80% acetonitrile, and demonstrated relationships of response factors with mobile phase composition and metabolite structural features including nonpolar surface areas, the length of the longest acyl chain, and the number of hydroxyl groups.
26331907	6	75	with	isomers	1675:1681	arg1	number					1697:1702	a common number	1688:1702	a common number of total acyl carbon atoms	1688:1729	This approach was employed to calculate predicted response factors for three authenticated acylsucroses based on mean values for all isomers with a common number of total acyl carbon atoms.
26331907	7	76	theme	wild	1839:1842	arg1	LA1777					1872:1877	the wild tomato Solanum habrochaites LA1777	1835:1877	the wild tomato Solanum habrochaites LA1777	1835:1877	Absolute UHPLC-MS quantification was performed on these three metabolites in an extract from leaves of the wild tomato Solanum habrochaites LA1777, yielding deviations of 26%, 6.7%, and 7.3% from values established using compound-specific response factors.
26331907	5	77	theme	mobile	1375:1380	arg1	phase					1382:1386	mobile phase composition and metabolite structural features including nonpolar surface areas, the length of the longest acyl chain, and the number of hydroxyl groups	1375:1539	phase	1382:1386	Once authenticated, molar response factors of [M + formate](-) ions for the acylsucrose metabolites were determined at different mobile phase compositions ranging from 40%-80% acetonitrile, and demonstrated relationships of response factors with mobile phase composition and metabolite structural features including nonpolar surface areas, the length of the longest acyl chain, and the number of hydroxyl groups.
26331907	5	78	theme	different	1248:1256	arg1	compositions					1271:1282	different mobile phase compositions	1248:1282	different mobile phase compositions ranging from 40%-80% acetonitrile	1248:1316	Once authenticated, molar response factors of [M + formate](-) ions for the acylsucrose metabolites were determined at different mobile phase compositions ranging from 40%-80% acetonitrile, and demonstrated relationships of response factors with mobile phase composition and metabolite structural features including nonpolar surface areas, the length of the longest acyl chain, and the number of hydroxyl groups.
26331907	4	79	theme	purified	837:844	arg1	metabolites					869:879	purified homologous acylsucrose metabolites	837:879	purified homologous acylsucrose metabolites	837:879	In order to lay a foundation for quantifying unknown neutral plant metabolites in absence of authentic standards, sub-milligram quantities of purified homologous acylsucrose metabolites were authenticated by subjecting each to basic hydrolysis and quantifying the sucrose product using stable-isotope dilution ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) with multiple reaction monitoring (MRM).
26331907	0	80	theme	newly-authenticated	125:143	arg1	metabolites					165:175	newly-authenticated neutral acylsucrose metabolites	125:175	newly-authenticated neutral acylsucrose metabolites	125:175	Dependence of negative-mode electrospray ionization response factors on mobile phase composition and molecular structure for newly-authenticated neutral acylsucrose metabolites.
26331907	4	81	theme	multiple	1093:1100	arg1	MRM					1123:1125	MRM	1123:1125	MRM	1123:1125	In order to lay a foundation for quantifying unknown neutral plant metabolites in absence of authentic standards, sub-milligram quantities of purified homologous acylsucrose metabolites were authenticated by subjecting each to basic hydrolysis and quantifying the sucrose product using stable-isotope dilution ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) with multiple reaction monitoring (MRM).
26331907	4	81	theme	multiple	1093:1100	arg1	monitoring					1111:1120	multiple reaction monitoring	1093:1120	multiple reaction monitoring (MRM)	1093:1126	In order to lay a foundation for quantifying unknown neutral plant metabolites in absence of authentic standards, sub-milligram quantities of purified homologous acylsucrose metabolites were authenticated by subjecting each to basic hydrolysis and quantifying the sucrose product using stable-isotope dilution ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) with multiple reaction monitoring (MRM).
26331907	4	82	theme	acylsucrose	857:867	arg1	metabolites					869:879	purified homologous acylsucrose metabolites	837:879	purified homologous acylsucrose metabolites	837:879	In order to lay a foundation for quantifying unknown neutral plant metabolites in absence of authentic standards, sub-milligram quantities of purified homologous acylsucrose metabolites were authenticated by subjecting each to basic hydrolysis and quantifying the sucrose product using stable-isotope dilution ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) with multiple reaction monitoring (MRM).
26331907	3	83	theme	metabolite	512:521	arg1	analyses					523:530	metabolite analyses	512:530	metabolite analyses	512:530	Despite the widespread use of electrospray ionization for metabolite analyses, the fundamentals needed for reliable prediction of molecular response factors have yet to be explored in detail for analytes that lack ionized functional groups.
26331907	2	84	theme	custom	436:441	arg1	syntheses					443:451	custom syntheses	436:451	custom syntheses	436:451	However, often such standards are not commercially available or not amenable for custom syntheses.
26331907	0	85	theme	electrospray	28:39	arg1	factors					61:67	negative-mode electrospray ionization response factors	14:67	negative-mode electrospray ionization response factors	14:67	Dependence of negative-mode electrospray ionization response factors on mobile phase composition and molecular structure for newly-authenticated neutral acylsucrose metabolites.
26331907	6	86	theme	carbon	1718:1723	arg1	atoms					1725:1729	total acyl carbon atoms	1707:1729	total acyl carbon atoms	1707:1729	This approach was employed to calculate predicted response factors for three authenticated acylsucroses based on mean values for all isomers with a common number of total acyl carbon atoms.
26331907	5	87	theme	nonpolar	1445:1452	arg1	areas					1462:1466	nonpolar surface areas	1445:1466	nonpolar surface areas	1445:1466	Once authenticated, molar response factors of [M + formate](-) ions for the acylsucrose metabolites were determined at different mobile phase compositions ranging from 40%-80% acetonitrile, and demonstrated relationships of response factors with mobile phase composition and metabolite structural features including nonpolar surface areas, the length of the longest acyl chain, and the number of hydroxyl groups.
26331907	0	88	theme	response	52:59	arg1	factors					61:67	negative-mode electrospray ionization response factors	14:67	negative-mode electrospray ionization response factors	14:67	Dependence of negative-mode electrospray ionization response factors on mobile phase composition and molecular structure for newly-authenticated neutral acylsucrose metabolites.
26331907	5	89	theme	molar	1149:1153	arg1	factors					1164:1170	molar response factors	1149:1170	molar response factors of [M + formate](-) ions for the acylsucrose metabolites	1149:1227	Once authenticated, molar response factors of [M + formate](-) ions for the acylsucrose metabolites were determined at different mobile phase compositions ranging from 40%-80% acetonitrile, and demonstrated relationships of response factors with mobile phase composition and metabolite structural features including nonpolar surface areas, the length of the longest acyl chain, and the number of hydroxyl groups.
26331907	7	90	from	values	1928:1933	arg1	deviations					1889:1898	deviations	1889:1898	deviations of 26%, 6.7%, and 7.3% from values established using compound-specific response factors	1889:1986	Absolute UHPLC-MS quantification was performed on these three metabolites in an extract from leaves of the wild tomato Solanum habrochaites LA1777, yielding deviations of 26%, 6.7%, and 7.3% from values established using compound-specific response factors.
26331907	5	91	theme	chain	1500:1504	arg1	number					1515:1520	the number	1511:1520	the number of hydroxyl groups	1511:1539	Once authenticated, molar response factors of [M + formate](-) ions for the acylsucrose metabolites were determined at different mobile phase compositions ranging from 40%-80% acetonitrile, and demonstrated relationships of response factors with mobile phase composition and metabolite structural features including nonpolar surface areas, the length of the longest acyl chain, and the number of hydroxyl groups.
26331907	5	91	theme	chain	1500:1504	arg1	areas					1462:1466	nonpolar surface areas	1445:1466	nonpolar surface areas	1445:1466	Once authenticated, molar response factors of [M + formate](-) ions for the acylsucrose metabolites were determined at different mobile phase compositions ranging from 40%-80% acetonitrile, and demonstrated relationships of response factors with mobile phase composition and metabolite structural features including nonpolar surface areas, the length of the longest acyl chain, and the number of hydroxyl groups.
26331907	5	91	theme	chain	1500:1504	arg1	length					1473:1478	the length	1469:1478	the length of the longest acyl chain	1469:1504	Once authenticated, molar response factors of [M + formate](-) ions for the acylsucrose metabolites were determined at different mobile phase compositions ranging from 40%-80% acetonitrile, and demonstrated relationships of response factors with mobile phase composition and metabolite structural features including nonpolar surface areas, the length of the longest acyl chain, and the number of hydroxyl groups.
26331907	5	92	theme	phase	1265:1269	arg1	compositions					1271:1282	different mobile phase compositions	1248:1282	different mobile phase compositions ranging from 40%-80% acetonitrile	1248:1316	Once authenticated, molar response factors of [M + formate](-) ions for the acylsucrose metabolites were determined at different mobile phase compositions ranging from 40%-80% acetonitrile, and demonstrated relationships of response factors with mobile phase composition and metabolite structural features including nonpolar surface areas, the length of the longest acyl chain, and the number of hydroxyl groups.
26331907	7	93	theme	response	1971:1978	arg1	factors					1980:1986	compound-specific response factors	1953:1986	compound-specific response factors	1953:1986	Absolute UHPLC-MS quantification was performed on these three metabolites in an extract from leaves of the wild tomato Solanum habrochaites LA1777, yielding deviations of 26%, 6.7%, and 7.3% from values established using compound-specific response factors.
26331907	3	94	theme	widespread	466:475	arg1	use					477:479	the widespread use	462:479	the widespread use of electrospray ionization for metabolite analyses	462:530	Despite the widespread use of electrospray ionization for metabolite analyses, the fundamentals needed for reliable prediction of molecular response factors have yet to be explored in detail for analytes that lack ionized functional groups.
26331907	4	95	theme	basic	922:926	arg1	hydrolysis					928:937	basic hydrolysis	922:937	basic hydrolysis	922:937	In order to lay a foundation for quantifying unknown neutral plant metabolites in absence of authentic standards, sub-milligram quantities of purified homologous acylsucrose metabolites were authenticated by subjecting each to basic hydrolysis and quantifying the sucrose product using stable-isotope dilution ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) with multiple reaction monitoring (MRM).
26331907	1	96	theme	plant	282:286	arg1	metabolites					288:298	plant metabolites	282:298	plant metabolites using liquid chromatography/mass spectrometry (LC/MS)	282:352	Authentic standards of known concentrations serve as references for accurate absolute quantification of plant metabolites using liquid chromatography/mass spectrometry (LC/MS).
26331907	5	97	theme	longest	1487:1493	arg1	chain					1500:1504	the longest acyl chain	1483:1504	the longest acyl chain	1483:1504	Once authenticated, molar response factors of [M + formate](-) ions for the acylsucrose metabolites were determined at different mobile phase compositions ranging from 40%-80% acetonitrile, and demonstrated relationships of response factors with mobile phase composition and metabolite structural features including nonpolar surface areas, the length of the longest acyl chain, and the number of hydroxyl groups.
26331907	3	98	theme	factors	603:609	arg1	prediction					570:579	reliable prediction	561:579	reliable prediction of molecular response factors	561:609	Despite the widespread use of electrospray ionization for metabolite analyses, the fundamentals needed for reliable prediction of molecular response factors have yet to be explored in detail for analytes that lack ionized functional groups.
26331907	6	99	theme	predicted	1582:1590	arg1	factors					1601:1607	predicted response factors	1582:1607	predicted response factors for three authenticated acylsucroses based on mean values for all isomers with a common number of total acyl carbon atoms	1582:1729	This approach was employed to calculate predicted response factors for three authenticated acylsucroses based on mean values for all isomers with a common number of total acyl carbon atoms.
26331907	5	100	theme	response	1353:1360	arg1	factors					1362:1368	response factors	1353:1368	response factors	1353:1368	Once authenticated, molar response factors of [M + formate](-) ions for the acylsucrose metabolites were determined at different mobile phase compositions ranging from 40%-80% acetonitrile, and demonstrated relationships of response factors with mobile phase composition and metabolite structural features including nonpolar surface areas, the length of the longest acyl chain, and the number of hydroxyl groups.
28911623	0	0	theme	correspondences	79:93	arg1	study					14:18	A comparative study	0:18	A comparative study of three tissue-cultured Dendrobium species and their wild correspondences by headspace gas chromatography-mass spectrometry	0:143	A comparative study of three tissue-cultured Dendrobium species and their wild correspondences by headspace gas chromatography-mass spectrometry combined with chemometric methods.
28911623	1	1	theme	Plant	180:184	arg1	technique					201:209	Plant tissue culture technique	180:209	Plant tissue culture technique	180:209	Plant tissue culture technique is widely used in the conservation and utilization of rare and endangered medicinal plants and it is crucial for tissue culture stocks to obtain the ability to produce similar bioactive components as their wild correspondences.
28911623	0	2	theme	wild	74:77	arg1	correspondences					79:93	their wild correspondences	68:93	their wild correspondences	68:93	A comparative study of three tissue-cultured Dendrobium species and their wild correspondences by headspace gas chromatography-mass spectrometry combined with chemometric methods.
28911623	2	3	theme	Migo	702:705	arg1	moniliforme					722:732	tissue-cultured and wild Dendrobium huoshanense Cheng and Tang, Dendrobium officinale Kimura et Migo and Dendrobium moniliforme	606:732	tissue-cultured and wild Dendrobium huoshanense Cheng and Tang, Dendrobium officinale Kimura et Migo and Dendrobium moniliforme	606:732	In this paper, a headspace gas chromatography-mass spectrometry method combined with chemometric methods was applied to analyze and evaluate the volatile compounds in tissue-cultured and wild Dendrobium huoshanense Cheng and Tang, Dendrobium officinale Kimura et Migo and Dendrobium moniliforme (Linn.)
28911623	1	4	theme	tissue	186:191	arg1	technique					201:209	Plant tissue culture technique	180:209	Plant tissue culture technique	180:209	Plant tissue culture technique is widely used in the conservation and utilization of rare and endangered medicinal plants and it is crucial for tissue culture stocks to obtain the ability to produce similar bioactive components as their wild correspondences.
28911623	1	5	theme	endangered	274:283	arg1	plants					295:300	rare and endangered medicinal plants	265:300	rare and endangered medicinal plants	265:300	Plant tissue culture technique is widely used in the conservation and utilization of rare and endangered medicinal plants and it is crucial for tissue culture stocks to obtain the ability to produce similar bioactive components as their wild correspondences.
28911623	0	6	theme	headspace	98:106	arg1	spectrometry					132:143	headspace gas chromatography-mass spectrometry	98:143	headspace gas chromatography-mass spectrometry	98:143	A comparative study of three tissue-cultured Dendrobium species and their wild correspondences by headspace gas chromatography-mass spectrometry combined with chemometric methods.
28911623	8	7	theme	spectrometry	1311:1322	arg1	strategy					1386:1393	an effective strategy	1373:1393	an effective strategy for identifying the species and their provenance	1373:1442	Gas chromatography-mass spectrometry techniques, combined with chemometrics, might be an effective strategy for identifying the species and their provenance, especially in the assessment of tissue-cultured Dendrobium quality for use in raw herbal medicines.
28911623	8	7	theme	spectrometry	1311:1322	arg1	techniques					1324:1333	Gas chromatography-mass spectrometry techniques	1287:1333	Gas chromatography-mass spectrometry techniques	1287:1333	Gas chromatography-mass spectrometry techniques, combined with chemometrics, might be an effective strategy for identifying the species and their provenance, especially in the assessment of tissue-cultured Dendrobium quality for use in raw herbal medicines.
28911623	1	8	theme	medicinal	285:293	arg1	plants					295:300	rare and endangered medicinal plants	265:300	rare and endangered medicinal plants	265:300	Plant tissue culture technique is widely used in the conservation and utilization of rare and endangered medicinal plants and it is crucial for tissue culture stocks to obtain the ability to produce similar bioactive components as their wild correspondences.
28911623	2	9	theme	wild	626:629	arg1	moniliforme					722:732	tissue-cultured and wild Dendrobium huoshanense Cheng and Tang, Dendrobium officinale Kimura et Migo and Dendrobium moniliforme	606:732	tissue-cultured and wild Dendrobium huoshanense Cheng and Tang, Dendrobium officinale Kimura et Migo and Dendrobium moniliforme	606:732	In this paper, a headspace gas chromatography-mass spectrometry method combined with chemometric methods was applied to analyze and evaluate the volatile compounds in tissue-cultured and wild Dendrobium huoshanense Cheng and Tang, Dendrobium officinale Kimura et Migo and Dendrobium moniliforme (Linn.)
28911623	5	10	contain	had	895:897	arg1	provenances					868:878	SAMPLES Different provenances	850:878	SAMPLES Different provenances of Dendrobiums	850:893	SAMPLES Different provenances of Dendrobiums had characteristic chemicals and showed remarkable quantity discrepancy of common compositions.
28911623	5	10	contain	had	895:897	arg2	chemicals					914:922	characteristic chemicals	899:922	characteristic chemicals	899:922	SAMPLES Different provenances of Dendrobiums had characteristic chemicals and showed remarkable quantity discrepancy of common compositions.
28911623	5	11	theme	remarkable	935:944	arg1	discrepancy					955:965	remarkable quantity discrepancy	935:965	remarkable quantity discrepancy of common compositions	935:988	SAMPLES Different provenances of Dendrobiums had characteristic chemicals and showed remarkable quantity discrepancy of common compositions.
28911623	8	12	from	strategy	1386:1393	arg1	assessment					1463:1472	the assessment	1459:1472	the assessment of tissue-cultured Dendrobium quality for use in raw herbal medicines	1459:1542	Gas chromatography-mass spectrometry techniques, combined with chemometrics, might be an effective strategy for identifying the species and their provenance, especially in the assessment of tissue-cultured Dendrobium quality for use in raw herbal medicines.
28911623	1	13	theme	plants	295:300	arg1	utilization					250:260	utilization	250:260	utilization	250:260	Plant tissue culture technique is widely used in the conservation and utilization of rare and endangered medicinal plants and it is crucial for tissue culture stocks to obtain the ability to produce similar bioactive components as their wild correspondences.
28911623	1	13	theme	plants	295:300	arg1	conservation					233:244	conservation	233:244	conservation	233:244	Plant tissue culture technique is widely used in the conservation and utilization of rare and endangered medicinal plants and it is crucial for tissue culture stocks to obtain the ability to produce similar bioactive components as their wild correspondences.
28911623	0	14	theme	chromatography-mass	112:130	arg1	spectrometry					132:143	headspace gas chromatography-mass spectrometry	98:143	headspace gas chromatography-mass spectrometry	98:143	A comparative study of three tissue-cultured Dendrobium species and their wild correspondences by headspace gas chromatography-mass spectrometry combined with chemometric methods.
28911623	8	15	from	use	1516:1518	arg1	medicines					1534:1542	raw herbal medicines	1523:1542	raw herbal medicines	1523:1542	Gas chromatography-mass spectrometry techniques, combined with chemometrics, might be an effective strategy for identifying the species and their provenance, especially in the assessment of tissue-cultured Dendrobium quality for use in raw herbal medicines.
28911623	2	16	dep	applied	548:554	arg1	Linn					735:738	Linn	735:738	Linn	735:738	In this paper, a headspace gas chromatography-mass spectrometry method combined with chemometric methods was applied to analyze and evaluate the volatile compounds in tissue-cultured and wild Dendrobium huoshanense Cheng and Tang, Dendrobium officinale Kimura et Migo and Dendrobium moniliforme (Linn.)
28911623	1	17	theme	culture	193:199	arg1	technique					201:209	Plant tissue culture technique	180:209	Plant tissue culture technique	180:209	Plant tissue culture technique is widely used in the conservation and utilization of rare and endangered medicinal plants and it is crucial for tissue culture stocks to obtain the ability to produce similar bioactive components as their wild correspondences.
28911623	0	18	theme	gas	108:110	arg1	spectrometry					132:143	headspace gas chromatography-mass spectrometry	98:143	headspace gas chromatography-mass spectrometry	98:143	A comparative study of three tissue-cultured Dendrobium species and their wild correspondences by headspace gas chromatography-mass spectrometry combined with chemometric methods.
28911623	1	19	theme	similar	379:385	arg1	components					397:406	similar bioactive components	379:406	similar bioactive components	379:406	Plant tissue culture technique is widely used in the conservation and utilization of rare and endangered medicinal plants and it is crucial for tissue culture stocks to obtain the ability to produce similar bioactive components as their wild correspondences.
28911623	1	19	theme	similar	379:385	arg1	correspondences					422:436	their wild correspondences	411:436	their wild correspondences	411:436	Plant tissue culture technique is widely used in the conservation and utilization of rare and endangered medicinal plants and it is crucial for tissue culture stocks to obtain the ability to produce similar bioactive components as their wild correspondences.
28911623	7	20	theme	data	1219:1222	arg1	variance					1224:1231	data variance	1219:1231	data variance	1219:1231	Principal component analysis showed that the first three components explained 85.9% of data variance, demonstrating a good discrimination between samples.
28911623	7	21	theme	good	1250:1253	arg1	discrimination					1255:1268	a good discrimination	1248:1268	a good discrimination between samples	1248:1284	Principal component analysis showed that the first three components explained 85.9% of data variance, demonstrating a good discrimination between samples.
28911623	8	22	theme	quality	1504:1510	arg1	assessment					1463:1472	the assessment	1459:1472	the assessment of tissue-cultured Dendrobium quality for use in raw herbal medicines	1459:1542	Gas chromatography-mass spectrometry techniques, combined with chemometrics, might be an effective strategy for identifying the species and their provenance, especially in the assessment of tissue-cultured Dendrobium quality for use in raw herbal medicines.
28911623	0	23	theme	comparative	2:12	arg1	study					14:18	A comparative study	0:18	A comparative study of three tissue-cultured Dendrobium species and their wild correspondences by headspace gas chromatography-mass spectrometry	0:143	A comparative study of three tissue-cultured Dendrobium species and their wild correspondences by headspace gas chromatography-mass spectrometry combined with chemometric methods.
28911623	1	24	theme	bioactive	387:395	arg1	components					397:406	similar bioactive components	379:406	similar bioactive components	379:406	Plant tissue culture technique is widely used in the conservation and utilization of rare and endangered medicinal plants and it is crucial for tissue culture stocks to obtain the ability to produce similar bioactive components as their wild correspondences.
28911623	1	24	theme	bioactive	387:395	arg1	correspondences					422:436	their wild correspondences	411:436	their wild correspondences	411:436	Plant tissue culture technique is widely used in the conservation and utilization of rare and endangered medicinal plants and it is crucial for tissue culture stocks to obtain the ability to produce similar bioactive components as their wild correspondences.
28911623	4	25	theme	volatile	759:766	arg1	compounds					768:776	63 volatile compounds	756:776	63 volatile compounds	756:776	In total, 63 volatile compounds were separated, with 53 being identified from the three Dendrobium spp.
28911623	5	26	theme	characteristic	899:912	arg1	chemicals					914:922	characteristic chemicals	899:922	characteristic chemicals	899:922	SAMPLES Different provenances of Dendrobiums had characteristic chemicals and showed remarkable quantity discrepancy of common compositions.
28911623	6	27	from	accumulation	1036:1047	arg1	samples					1085:1091	Dendrobium samples	1074:1091	Dendrobium samples	1074:1091	The similarity evaluation disclosed that the accumulation of volatile compounds in Dendrobium samples might be affected by their provenance.
28911623	7	28	theme	variance	1224:1231	arg1	%					1214:1214	85.9%	1210:1214	85.9% of data variance	1210:1231	Principal component analysis showed that the first three components explained 85.9% of data variance, demonstrating a good discrimination between samples.
28911623	7	28	theme	variance	1224:1231	arg1	variance					1224:1231	data variance	1219:1231	data variance	1219:1231	Principal component analysis showed that the first three components explained 85.9% of data variance, demonstrating a good discrimination between samples.
28911623	2	29	theme	Dendrobium	711:720	arg1	moniliforme					722:732	tissue-cultured and wild Dendrobium huoshanense Cheng and Tang, Dendrobium officinale Kimura et Migo and Dendrobium moniliforme	606:732	tissue-cultured and wild Dendrobium huoshanense Cheng and Tang, Dendrobium officinale Kimura et Migo and Dendrobium moniliforme	606:732	In this paper, a headspace gas chromatography-mass spectrometry method combined with chemometric methods was applied to analyze and evaluate the volatile compounds in tissue-cultured and wild Dendrobium huoshanense Cheng and Tang, Dendrobium officinale Kimura et Migo and Dendrobium moniliforme (Linn.)
28911623	7	30	theme	component	1142:1150	arg1	analysis					1152:1159	Principal component analysis	1132:1159	Principal component analysis	1132:1159	Principal component analysis showed that the first three components explained 85.9% of data variance, demonstrating a good discrimination between samples.
28911623	2	31	theme	volatile	584:591	arg1	compounds					593:601	the volatile compounds	580:601	the volatile compounds in tissue-cultured and wild Dendrobium huoshanense Cheng and Tang, Dendrobium officinale Kimura et Migo and Dendrobium moniliforme	580:732	In this paper, a headspace gas chromatography-mass spectrometry method combined with chemometric methods was applied to analyze and evaluate the volatile compounds in tissue-cultured and wild Dendrobium huoshanense Cheng and Tang, Dendrobium officinale Kimura et Migo and Dendrobium moniliforme (Linn.)
28911623	5	32	theme	compositions	977:988	arg1	discrepancy					955:965	remarkable quantity discrepancy	935:965	remarkable quantity discrepancy of common compositions	935:988	SAMPLES Different provenances of Dendrobiums had characteristic chemicals and showed remarkable quantity discrepancy of common compositions.
28911623	8	33	theme	chromatography-mass	1291:1309	arg1	spectrometry					1311:1322	Gas chromatography-mass spectrometry	1287:1322	Gas chromatography-mass spectrometry techniques	1287:1333	Gas chromatography-mass spectrometry techniques, combined with chemometrics, might be an effective strategy for identifying the species and their provenance, especially in the assessment of tissue-cultured Dendrobium quality for use in raw herbal medicines.
28911623	7	34	theme	first	1177:1181	arg1	components					1189:1198	the first three components	1173:1198	the first three components	1173:1198	Principal component analysis showed that the first three components explained 85.9% of data variance, demonstrating a good discrimination between samples.
28911623	5	35	theme	SAMPLES	850:856	arg1	provenances					868:878	SAMPLES Different provenances	850:878	SAMPLES Different provenances of Dendrobiums	850:893	SAMPLES Different provenances of Dendrobiums had characteristic chemicals and showed remarkable quantity discrepancy of common compositions.
28911623	5	36	theme	quantity	946:953	arg1	discrepancy					955:965	remarkable quantity discrepancy	935:965	remarkable quantity discrepancy of common compositions	935:988	SAMPLES Different provenances of Dendrobiums had characteristic chemicals and showed remarkable quantity discrepancy of common compositions.
28911623	5	37	theme	Different	858:866	arg1	provenances					868:878	SAMPLES Different provenances	850:878	SAMPLES Different provenances of Dendrobiums	850:893	SAMPLES Different provenances of Dendrobiums had characteristic chemicals and showed remarkable quantity discrepancy of common compositions.
28911623	0	38	theme	tissue-cultured	29:43	arg1	species					56:62	three tissue-cultured Dendrobium species	23:62	three tissue-cultured Dendrobium species	23:62	A comparative study of three tissue-cultured Dendrobium species and their wild correspondences by headspace gas chromatography-mass spectrometry combined with chemometric methods.
28911623	0	39	theme	chemometric	159:169	arg1	methods					171:177	chemometric methods	159:177	chemometric methods	159:177	A comparative study of three tissue-cultured Dendrobium species and their wild correspondences by headspace gas chromatography-mass spectrometry combined with chemometric methods.
28911623	4	40	theme	Dendrobium	834:843	arg1	spp					845:847	the three Dendrobium spp	824:847	the three Dendrobium spp	824:847	In total, 63 volatile compounds were separated, with 53 being identified from the three Dendrobium spp.
28911623	2	41	theme	spectrometry	490:501	arg1	method					503:508	a headspace gas chromatography-mass spectrometry method	454:508	a headspace gas chromatography-mass spectrometry method combined with chemometric methods	454:542	In this paper, a headspace gas chromatography-mass spectrometry method combined with chemometric methods was applied to analyze and evaluate the volatile compounds in tissue-cultured and wild Dendrobium huoshanense Cheng and Tang, Dendrobium officinale Kimura et Migo and Dendrobium moniliforme (Linn.)
28911623	2	42	from	compounds	593:601	arg1	moniliforme					722:732	tissue-cultured and wild Dendrobium huoshanense Cheng and Tang, Dendrobium officinale Kimura et Migo and Dendrobium moniliforme	606:732	tissue-cultured and wild Dendrobium huoshanense Cheng and Tang, Dendrobium officinale Kimura et Migo and Dendrobium moniliforme	606:732	In this paper, a headspace gas chromatography-mass spectrometry method combined with chemometric methods was applied to analyze and evaluate the volatile compounds in tissue-cultured and wild Dendrobium huoshanense Cheng and Tang, Dendrobium officinale Kimura et Migo and Dendrobium moniliforme (Linn.)
28911623	6	43	theme	compounds	1061:1069	arg1	accumulation					1036:1047	the accumulation	1032:1047	the accumulation of volatile compounds in Dendrobium samples	1032:1091	The similarity evaluation disclosed that the accumulation of volatile compounds in Dendrobium samples might be affected by their provenance.
28911623	8	44	theme	Dendrobium	1493:1502	arg1	quality					1504:1510	tissue-cultured Dendrobium quality	1477:1510	tissue-cultured Dendrobium quality	1477:1510	Gas chromatography-mass spectrometry techniques, combined with chemometrics, might be an effective strategy for identifying the species and their provenance, especially in the assessment of tissue-cultured Dendrobium quality for use in raw herbal medicines.
28911623	0	45	theme	species	56:62	arg1	study					14:18	A comparative study	0:18	A comparative study of three tissue-cultured Dendrobium species and their wild correspondences by headspace gas chromatography-mass spectrometry	0:143	A comparative study of three tissue-cultured Dendrobium species and their wild correspondences by headspace gas chromatography-mass spectrometry combined with chemometric methods.
28911623	1	46	theme	tissue	324:329	arg1	stocks					339:344	tissue culture stocks	324:344	tissue culture stocks	324:344	Plant tissue culture technique is widely used in the conservation and utilization of rare and endangered medicinal plants and it is crucial for tissue culture stocks to obtain the ability to produce similar bioactive components as their wild correspondences.
28911623	8	47	theme	Gas	1287:1289	arg1	spectrometry					1311:1322	Gas chromatography-mass spectrometry	1287:1322	Gas chromatography-mass spectrometry techniques	1287:1333	Gas chromatography-mass spectrometry techniques, combined with chemometrics, might be an effective strategy for identifying the species and their provenance, especially in the assessment of tissue-cultured Dendrobium quality for use in raw herbal medicines.
28911623	1	48	theme	wild	417:420	arg1	components					397:406	similar bioactive components	379:406	similar bioactive components	379:406	Plant tissue culture technique is widely used in the conservation and utilization of rare and endangered medicinal plants and it is crucial for tissue culture stocks to obtain the ability to produce similar bioactive components as their wild correspondences.
28911623	1	48	theme	wild	417:420	arg1	correspondences					422:436	their wild correspondences	411:436	their wild correspondences	411:436	Plant tissue culture technique is widely used in the conservation and utilization of rare and endangered medicinal plants and it is crucial for tissue culture stocks to obtain the ability to produce similar bioactive components as their wild correspondences.
28911623	7	49	theme	Principal	1132:1140	arg1	analysis					1152:1159	Principal component analysis	1132:1159	Principal component analysis	1132:1159	Principal component analysis showed that the first three components explained 85.9% of data variance, demonstrating a good discrimination between samples.
28911623	0	50	theme	Dendrobium	45:54	arg1	species					56:62	three tissue-cultured Dendrobium species	23:62	three tissue-cultured Dendrobium species	23:62	A comparative study of three tissue-cultured Dendrobium species and their wild correspondences by headspace gas chromatography-mass spectrometry combined with chemometric methods.
28911623	1	51	theme	culture	331:337	arg1	stocks					339:344	tissue culture stocks	324:344	tissue culture stocks	324:344	Plant tissue culture technique is widely used in the conservation and utilization of rare and endangered medicinal plants and it is crucial for tissue culture stocks to obtain the ability to produce similar bioactive components as their wild correspondences.
28911623	2	52	theme	tissue-cultured	606:620	arg1	moniliforme					722:732	tissue-cultured and wild Dendrobium huoshanense Cheng and Tang, Dendrobium officinale Kimura et Migo and Dendrobium moniliforme	606:732	tissue-cultured and wild Dendrobium huoshanense Cheng and Tang, Dendrobium officinale Kimura et Migo and Dendrobium moniliforme	606:732	In this paper, a headspace gas chromatography-mass spectrometry method combined with chemometric methods was applied to analyze and evaluate the volatile compounds in tissue-cultured and wild Dendrobium huoshanense Cheng and Tang, Dendrobium officinale Kimura et Migo and Dendrobium moniliforme (Linn.)
28911623	2	53	theme	gas	466:468	arg1	spectrometry					490:501	headspace gas chromatography-mass spectrometry	456:501	a headspace gas chromatography-mass spectrometry method combined with chemometric methods	454:542	In this paper, a headspace gas chromatography-mass spectrometry method combined with chemometric methods was applied to analyze and evaluate the volatile compounds in tissue-cultured and wild Dendrobium huoshanense Cheng and Tang, Dendrobium officinale Kimura et Migo and Dendrobium moniliforme (Linn.)
28911623	1	54	used	used	221:224	arg2	technique					201:209	Plant tissue culture technique	180:209	Plant tissue culture technique	180:209	Plant tissue culture technique is widely used in the conservation and utilization of rare and endangered medicinal plants and it is crucial for tissue culture stocks to obtain the ability to produce similar bioactive components as their wild correspondences.
28911623	8	55	theme	effective	1376:1384	arg1	strategy					1386:1393	an effective strategy	1373:1393	an effective strategy for identifying the species and their provenance	1373:1442	Gas chromatography-mass spectrometry techniques, combined with chemometrics, might be an effective strategy for identifying the species and their provenance, especially in the assessment of tissue-cultured Dendrobium quality for use in raw herbal medicines.
28911623	8	55	theme	effective	1376:1384	arg1	techniques					1324:1333	Gas chromatography-mass spectrometry techniques	1287:1333	Gas chromatography-mass spectrometry techniques	1287:1333	Gas chromatography-mass spectrometry techniques, combined with chemometrics, might be an effective strategy for identifying the species and their provenance, especially in the assessment of tissue-cultured Dendrobium quality for use in raw herbal medicines.
28911623	8	56	theme	herbal	1527:1532	arg1	medicines					1534:1542	raw herbal medicines	1523:1542	raw herbal medicines	1523:1542	Gas chromatography-mass spectrometry techniques, combined with chemometrics, might be an effective strategy for identifying the species and their provenance, especially in the assessment of tissue-cultured Dendrobium quality for use in raw herbal medicines.
28911623	8	57	theme	raw	1523:1525	arg1	medicines					1534:1542	raw herbal medicines	1523:1542	raw herbal medicines	1523:1542	Gas chromatography-mass spectrometry techniques, combined with chemometrics, might be an effective strategy for identifying the species and their provenance, especially in the assessment of tissue-cultured Dendrobium quality for use in raw herbal medicines.
28911623	5	58	theme	common	970:975	arg1	compositions					977:988	common compositions	970:988	common compositions	970:988	SAMPLES Different provenances of Dendrobiums had characteristic chemicals and showed remarkable quantity discrepancy of common compositions.
28911623	8	59	theme	tissue-cultured	1477:1491	arg1	quality					1504:1510	tissue-cultured Dendrobium quality	1477:1510	tissue-cultured Dendrobium quality	1477:1510	Gas chromatography-mass spectrometry techniques, combined with chemometrics, might be an effective strategy for identifying the species and their provenance, especially in the assessment of tissue-cultured Dendrobium quality for use in raw herbal medicines.
28911623	6	60	theme	volatile	1052:1059	arg1	compounds					1061:1069	volatile compounds	1052:1069	volatile compounds	1052:1069	The similarity evaluation disclosed that the accumulation of volatile compounds in Dendrobium samples might be affected by their provenance.
28911623	1	61	dep	conservation	233:244	arg1	the					229:231	the	229:231	the	229:231	Plant tissue culture technique is widely used in the conservation and utilization of rare and endangered medicinal plants and it is crucial for tissue culture stocks to obtain the ability to produce similar bioactive components as their wild correspondences.
28911623	5	62	theme	Dendrobiums	883:893	arg1	provenances					868:878	SAMPLES Different provenances	850:878	SAMPLES Different provenances of Dendrobiums	850:893	SAMPLES Different provenances of Dendrobiums had characteristic chemicals and showed remarkable quantity discrepancy of common compositions.
28911623	2	63	theme	chemometric	524:534	arg1	methods					536:542	chemometric methods	524:542	chemometric methods	524:542	In this paper, a headspace gas chromatography-mass spectrometry method combined with chemometric methods was applied to analyze and evaluate the volatile compounds in tissue-cultured and wild Dendrobium huoshanense Cheng and Tang, Dendrobium officinale Kimura et Migo and Dendrobium moniliforme (Linn.)
28911623	2	64	theme	chromatography-mass	470:488	arg1	spectrometry					490:501	headspace gas chromatography-mass spectrometry	456:501	a headspace gas chromatography-mass spectrometry method combined with chemometric methods	454:542	In this paper, a headspace gas chromatography-mass spectrometry method combined with chemometric methods was applied to analyze and evaluate the volatile compounds in tissue-cultured and wild Dendrobium huoshanense Cheng and Tang, Dendrobium officinale Kimura et Migo and Dendrobium moniliforme (Linn.)
28911623	6	65	theme	Dendrobium	1074:1083	arg1	samples					1085:1091	Dendrobium samples	1074:1091	Dendrobium samples	1074:1091	The similarity evaluation disclosed that the accumulation of volatile compounds in Dendrobium samples might be affected by their provenance.
28911623	2	66	theme	headspace	456:464	arg1	spectrometry					490:501	headspace gas chromatography-mass spectrometry	456:501	a headspace gas chromatography-mass spectrometry method combined with chemometric methods	454:542	In this paper, a headspace gas chromatography-mass spectrometry method combined with chemometric methods was applied to analyze and evaluate the volatile compounds in tissue-cultured and wild Dendrobium huoshanense Cheng and Tang, Dendrobium officinale Kimura et Migo and Dendrobium moniliforme (Linn.)
28911623	6	67	theme	similarity	995:1004	arg1	evaluation					1006:1015	The similarity evaluation	991:1015	The similarity evaluation	991:1015	The similarity evaluation disclosed that the accumulation of volatile compounds in Dendrobium samples might be affected by their provenance.
28911623	1	68	theme	rare	265:268	arg1	plants					295:300	rare and endangered medicinal plants	265:300	rare and endangered medicinal plants	265:300	Plant tissue culture technique is widely used in the conservation and utilization of rare and endangered medicinal plants and it is crucial for tissue culture stocks to obtain the ability to produce similar bioactive components as their wild correspondences.
25421533	3	0	theme	polyphenols	321:331	arg1	Levels					311:316	Levels	311:316	Levels of polyphenols, flavonoids and polysaccharides	311:363	Levels of polyphenols, flavonoids and polysaccharides and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free-radical scavenging activity were measured in extracts of P. alte-lobatum (EPA).
25421533	3	0	theme	polyphenols	321:331	arg1	activity					430:437	2,2-diphenyl-1-picrylhydrazyl (DPPH) free-radical scavenging activity	369:437	2,2-diphenyl-1-picrylhydrazyl (DPPH) free-radical scavenging activity	369:437	Levels of polyphenols, flavonoids and polysaccharides and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free-radical scavenging activity were measured in extracts of P. alte-lobatum (EPA).
25421533	8	1	theme	endurance	1135:1143	arg1	time					1153:1156	endurance running time	1135:1156	endurance running time to exhaustion and superoxide dismutase activity	1135:1204	EPA treatment dose-dependently increased endurance running time to exhaustion and superoxide dismutase activity and total antioxidant ability of blood.
25421533	10	2	theme	important	1369:1377	arg1	source					1386:1391	an important energy source	1366:1391	an important energy source for exercise	1366:1404	Hepatic glycogen content, an important energy source for exercise, was significantly increased with EPA treatment.
25421533	10	2	theme	important	1369:1377	arg1	content					1357:1363	Hepatic glycogen content	1340:1363	Hepatic glycogen content	1340:1363	Hepatic glycogen content, an important energy source for exercise, was significantly increased with EPA treatment.
25421533	1	3	dep	Polygonatum	92:102	arg1	Hayata					117:122	Polygonatum alte-lobatum Hayata	92:122	Polygonatum alte-lobatum Hayata	92:122	Polygonatum alte-lobatum Hayata, a rhizomatous perennial herb, belongs to the Liliaceae family and is endemic to Taiwan.
25421533	6	4	dep	times	959:963	arg1	greater					965:971	greater	965:971	greater	965:971	EPA contained polyphenols, flavonoids and polysaccharides, with polysaccharide content at least 26 times greater than that of polyphenols and flavonoids.
25421533	9	5	theme	serum	1277:1281	arg1	nitrogen					1288:1295	serum urea nitrogen and malondialdehyde levels	1277:1322	nitrogen	1288:1295	EPA dose-dependently decreased serum urea nitrogen and malondialdehyde levels after exercise.
25421533	8	6	theme	antioxidant	1216:1226	arg1	ability					1228:1234	total antioxidant ability	1210:1234	total antioxidant ability of blood	1210:1243	EPA treatment dose-dependently increased endurance running time to exhaustion and superoxide dismutase activity and total antioxidant ability of blood.
25421533	5	7	theme	experiment	848:857	arg1	end					837:839	the end	833:839	the end of the experiment	833:857	Exercise performance was evaluated by exhaustive treadmill exercise time and by changes in body composition and biochemical variables at the end of the experiment.
25421533	4	8	with	treatment	561:569	arg1	control					585:591	control	585:591	control	585:591	Sprague-Dawley rats were randomly divided into four groups for 8-week treatment with vehicle (control) and low-, medium-, and high-dose EPA (LEPA, MEPA, HEPA; 0, 75, 150, and 375 mg/kg/day, respectively).
25421533	4	8	with	treatment	561:569	arg1	low-					598:601	low-	598:601	low-	598:601	Sprague-Dawley rats were randomly divided into four groups for 8-week treatment with vehicle (control) and low-, medium-, and high-dose EPA (LEPA, MEPA, HEPA; 0, 75, 150, and 375 mg/kg/day, respectively).
25421533	4	8	with	treatment	561:569	arg1	vehicle					576:582	vehicle	576:582	vehicle (control)	576:592	Sprague-Dawley rats were randomly divided into four groups for 8-week treatment with vehicle (control) and low-, medium-, and high-dose EPA (LEPA, MEPA, HEPA; 0, 75, 150, and 375 mg/kg/day, respectively).
25421533	10	9	theme	EPA	1440:1442	arg1	treatment					1444:1452	EPA treatment	1440:1452	EPA treatment	1440:1452	Hepatic glycogen content, an important energy source for exercise, was significantly increased with EPA treatment.
25421533	7	10	theme	free	1079:1082	arg1	radicals					1084:1091	DPPH free radicals	1074:1091	DPPH free radicals	1074:1091	Trend analysis revealed that EPA dose-dependently scavenged DPPH free radicals.
25421533	2	11	theme	exercised	295:303	arg1	rats					305:308	exercised rats	295:308	exercised rats	295:308	We investigated the antioxidant and anti-fatigue activities of P. alte-lobatum in exercised rats.
25421533	8	12	theme	running	1145:1151	arg1	time					1153:1156	endurance running time	1135:1156	endurance running time to exhaustion and superoxide dismutase activity	1135:1204	EPA treatment dose-dependently increased endurance running time to exhaustion and superoxide dismutase activity and total antioxidant ability of blood.
25421533	5	13	from	end	837:839	arg1	time					764:767	exhaustive treadmill exercise time	734:767	exhaustive treadmill exercise time	734:767	Exercise performance was evaluated by exhaustive treadmill exercise time and by changes in body composition and biochemical variables at the end of the experiment.
25421533	0	14	theme	Antioxidant	0:10	arg1	activities					28:37	Antioxidant and antifatigue activities	0:37	Antioxidant and antifatigue activities of Polygonatum Alte-lobatum Hayata rhizomes in rats.	0:90	Antioxidant and antifatigue activities of Polygonatum Alte-lobatum Hayata rhizomes in rats.
25421533	5	15	theme	biochemical	808:818	arg1	variables					820:828	biochemical variables	808:828	biochemical variables	808:828	Exercise performance was evaluated by exhaustive treadmill exercise time and by changes in body composition and biochemical variables at the end of the experiment.
25421533	3	16	theme	polysaccharides	349:363	arg1	Levels					311:316	Levels	311:316	Levels of polyphenols, flavonoids and polysaccharides	311:363	Levels of polyphenols, flavonoids and polysaccharides and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free-radical scavenging activity were measured in extracts of P. alte-lobatum (EPA).
25421533	3	16	theme	polysaccharides	349:363	arg1	activity					430:437	2,2-diphenyl-1-picrylhydrazyl (DPPH) free-radical scavenging activity	369:437	2,2-diphenyl-1-picrylhydrazyl (DPPH) free-radical scavenging activity	369:437	Levels of polyphenols, flavonoids and polysaccharides and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free-radical scavenging activity were measured in extracts of P. alte-lobatum (EPA).
25421533	4	17	theme	Sprague-Dawley	491:504	arg1	rats					506:509	Sprague-Dawley rats	491:509	Sprague-Dawley rats	491:509	Sprague-Dawley rats were randomly divided into four groups for 8-week treatment with vehicle (control) and low-, medium-, and high-dose EPA (LEPA, MEPA, HEPA; 0, 75, 150, and 375 mg/kg/day, respectively).
25421533	2	18	theme	alte-lobatum	279:290	arg1	activities					262:271	the antioxidant and anti-fatigue activities	229:271	the antioxidant and anti-fatigue activities of P. alte-lobatum in exercised rats	229:308	We investigated the antioxidant and anti-fatigue activities of P. alte-lobatum in exercised rats.
25421533	1	19	theme	Liliaceae	170:178	arg1	family					180:185	the Liliaceae family	166:185	the Liliaceae family	166:185	Polygonatum alte-lobatum Hayata, a rhizomatous perennial herb, belongs to the Liliaceae family and is endemic to Taiwan.
25421533	5	20	theme	exercise	755:762	arg1	time					764:767	exhaustive treadmill exercise time	734:767	exhaustive treadmill exercise time	734:767	Exercise performance was evaluated by exhaustive treadmill exercise time and by changes in body composition and biochemical variables at the end of the experiment.
25421533	7	21	theme	DPPH	1074:1077	arg1	radicals					1084:1091	DPPH free radicals	1074:1091	DPPH free radicals	1074:1091	Trend analysis revealed that EPA dose-dependently scavenged DPPH free radicals.
25421533	2	22	theme	P.	276:277	arg1	alte-lobatum					279:290	P. alte-lobatum	276:290	P. alte-lobatum	276:290	We investigated the antioxidant and anti-fatigue activities of P. alte-lobatum in exercised rats.
25421533	3	23	theme	alte-lobatum	471:482	arg1	extracts					456:463	extracts	456:463	extracts of P. alte-lobatum (EPA)	456:488	Levels of polyphenols, flavonoids and polysaccharides and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free-radical scavenging activity were measured in extracts of P. alte-lobatum (EPA).
25421533	3	24	theme	free-radical	406:417	arg1	activity					430:437	2,2-diphenyl-1-picrylhydrazyl (DPPH) free-radical scavenging activity	369:437	2,2-diphenyl-1-picrylhydrazyl (DPPH) free-radical scavenging activity	369:437	Levels of polyphenols, flavonoids and polysaccharides and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free-radical scavenging activity were measured in extracts of P. alte-lobatum (EPA).
25421533	5	25	theme	Exercise	696:703	arg1	performance					705:715	Exercise performance	696:715	Exercise performance	696:715	Exercise performance was evaluated by exhaustive treadmill exercise time and by changes in body composition and biochemical variables at the end of the experiment.
25421533	5	26	from	changes	776:782	arg1	composition					792:802	body composition	787:802	body composition	787:802	Exercise performance was evaluated by exhaustive treadmill exercise time and by changes in body composition and biochemical variables at the end of the experiment.
25421533	5	26	from	changes	776:782	arg1	variables					820:828	biochemical variables	808:828	biochemical variables	808:828	Exercise performance was evaluated by exhaustive treadmill exercise time and by changes in body composition and biochemical variables at the end of the experiment.
25421533	6	27	contain	contained	864:872	arg2	polyphenols					874:884	polyphenols	874:884	polyphenols	874:884	EPA contained polyphenols, flavonoids and polysaccharides, with polysaccharide content at least 26 times greater than that of polyphenols and flavonoids.
25421533	6	27	contain	contained	864:872	arg1	EPA					860:862	EPA	860:862	EPA	860:862	EPA contained polyphenols, flavonoids and polysaccharides, with polysaccharide content at least 26 times greater than that of polyphenols and flavonoids.
25421533	6	27	contain	contained	864:872	arg2	polysaccharides					902:916	polysaccharides	902:916	polysaccharides	902:916	EPA contained polyphenols, flavonoids and polysaccharides, with polysaccharide content at least 26 times greater than that of polyphenols and flavonoids.
25421533	6	27	contain	contained	864:872	arg2	flavonoids					887:896	flavonoids	887:896	flavonoids	887:896	EPA contained polyphenols, flavonoids and polysaccharides, with polysaccharide content at least 26 times greater than that of polyphenols and flavonoids.
25421533	3	28	theme	scavenging	419:428	arg1	activity					430:437	2,2-diphenyl-1-picrylhydrazyl (DPPH) free-radical scavenging activity	369:437	2,2-diphenyl-1-picrylhydrazyl (DPPH) free-radical scavenging activity	369:437	Levels of polyphenols, flavonoids and polysaccharides and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free-radical scavenging activity were measured in extracts of P. alte-lobatum (EPA).
25421533	8	29	theme	blood	1239:1243	arg1	time					1153:1156	endurance running time	1135:1156	endurance running time to exhaustion and superoxide dismutase activity	1135:1204	EPA treatment dose-dependently increased endurance running time to exhaustion and superoxide dismutase activity and total antioxidant ability of blood.
25421533	8	29	theme	blood	1239:1243	arg1	ability					1228:1234	total antioxidant ability	1210:1234	total antioxidant ability of blood	1210:1243	EPA treatment dose-dependently increased endurance running time to exhaustion and superoxide dismutase activity and total antioxidant ability of blood.
25421533	1	30	theme	alte-lobatum	104:115	arg1	herb					149:152	a rhizomatous perennial herb	125:152	a rhizomatous perennial herb	125:152	Polygonatum alte-lobatum Hayata, a rhizomatous perennial herb, belongs to the Liliaceae family and is endemic to Taiwan.
25421533	1	30	theme	alte-lobatum	104:115	arg1	Polygonatum					92:102	Polygonatum alte-lobatum Hayata	92:122	Polygonatum alte-lobatum Hayata	92:122	Polygonatum alte-lobatum Hayata, a rhizomatous perennial herb, belongs to the Liliaceae family and is endemic to Taiwan.
25421533	10	31	theme	Hepatic	1340:1346	arg1	source					1386:1391	an important energy source	1366:1391	an important energy source for exercise	1366:1404	Hepatic glycogen content, an important energy source for exercise, was significantly increased with EPA treatment.
25421533	10	31	theme	Hepatic	1340:1346	arg1	content					1357:1363	Hepatic glycogen content	1340:1363	Hepatic glycogen content	1340:1363	Hepatic glycogen content, an important energy source for exercise, was significantly increased with EPA treatment.
25421533	6	32	theme	polysaccharide	924:937	arg1	content					939:945	polysaccharide content	924:945	polysaccharide content at least 26 times greater than that of polyphenols and flavonoids	924:1011	EPA contained polyphenols, flavonoids and polysaccharides, with polysaccharide content at least 26 times greater than that of polyphenols and flavonoids.
25421533	11	33	with	agent	1480:1484	arg1	function					1523:1530	an anti-fatigue pharmacological function	1491:1530	an anti-fatigue pharmacological function	1491:1530	EPA could be a potential agent with an anti-fatigue pharmacological function.
25421533	5	34	theme	treadmill	745:753	arg1	time					764:767	exhaustive treadmill exercise time	734:767	exhaustive treadmill exercise time	734:767	Exercise performance was evaluated by exhaustive treadmill exercise time and by changes in body composition and biochemical variables at the end of the experiment.
25421533	0	35	theme	antifatigue	16:26	arg1	activities					28:37	Antioxidant and antifatigue activities	0:37	Antioxidant and antifatigue activities of Polygonatum Alte-lobatum Hayata rhizomes in rats.	0:90	Antioxidant and antifatigue activities of Polygonatum Alte-lobatum Hayata rhizomes in rats.
25421533	11	36	theme	potential	1470:1478	arg1	agent					1480:1484	a potential agent	1468:1484	a potential agent with an anti-fatigue pharmacological function	1468:1530	EPA could be a potential agent with an anti-fatigue pharmacological function.
25421533	11	36	theme	potential	1470:1478	arg1	EPA					1455:1457	EPA	1455:1457	EPA	1455:1457	EPA could be a potential agent with an anti-fatigue pharmacological function.
25421533	5	37	theme	body	787:790	arg1	composition					792:802	body composition	787:802	body composition	787:802	Exercise performance was evaluated by exhaustive treadmill exercise time and by changes in body composition and biochemical variables at the end of the experiment.
25421533	0	38	theme	Polygonatum	42:52	arg1	rhizomes					74:81	Polygonatum Alte-lobatum Hayata rhizomes	42:81	Polygonatum Alte-lobatum Hayata rhizomes	42:81	Antioxidant and antifatigue activities of Polygonatum Alte-lobatum Hayata rhizomes in rats.
25421533	7	39	theme	Trend	1014:1018	arg1	analysis					1020:1027	Trend analysis	1014:1027	Trend analysis	1014:1027	Trend analysis revealed that EPA dose-dependently scavenged DPPH free radicals.
25421533	2	40	theme	antioxidant	233:243	arg1	activities					262:271	the antioxidant and anti-fatigue activities	229:271	the antioxidant and anti-fatigue activities of P. alte-lobatum in exercised rats	229:308	We investigated the antioxidant and anti-fatigue activities of P. alte-lobatum in exercised rats.
25421533	2	41	from	activities	262:271	arg1	rats					305:308	exercised rats	295:308	exercised rats	295:308	We investigated the antioxidant and anti-fatigue activities of P. alte-lobatum in exercised rats.
25421533	3	42	theme	P.	468:469	arg1	EPA					485:487	EPA	485:487	EPA	485:487	Levels of polyphenols, flavonoids and polysaccharides and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free-radical scavenging activity were measured in extracts of P. alte-lobatum (EPA).
25421533	3	42	theme	P.	468:469	arg1	alte-lobatum					471:482	P. alte-lobatum	468:482	P. alte-lobatum (EPA)	468:488	Levels of polyphenols, flavonoids and polysaccharides and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free-radical scavenging activity were measured in extracts of P. alte-lobatum (EPA).
25421533	4	43	theme	8-week	554:559	arg1	treatment					561:569	8-week treatment	554:569	8-week treatment with vehicle (control) and low-, medium-, and high-dose EPA (LEPA, MEPA, HEPA; 0, 75, 150, and 375 mg/kg/day, respectively)	554:693	Sprague-Dawley rats were randomly divided into four groups for 8-week treatment with vehicle (control) and low-, medium-, and high-dose EPA (LEPA, MEPA, HEPA; 0, 75, 150, and 375 mg/kg/day, respectively).
25421533	5	44	theme	exhaustive	734:743	arg1	time					764:767	exhaustive treadmill exercise time	734:767	exhaustive treadmill exercise time	734:767	Exercise performance was evaluated by exhaustive treadmill exercise time and by changes in body composition and biochemical variables at the end of the experiment.
25421533	4	45	dep	low-	598:601	arg1	HEPA					644:647	HEPA	644:647	HEPA	644:647	Sprague-Dawley rats were randomly divided into four groups for 8-week treatment with vehicle (control) and low-, medium-, and high-dose EPA (LEPA, MEPA, HEPA; 0, 75, 150, and 375 mg/kg/day, respectively).
25421533	4	45	dep	low-	598:601	arg1	MEPA					638:641	MEPA	638:641	MEPA	638:641	Sprague-Dawley rats were randomly divided into four groups for 8-week treatment with vehicle (control) and low-, medium-, and high-dose EPA (LEPA, MEPA, HEPA; 0, 75, 150, and 375 mg/kg/day, respectively).
25421533	4	45	dep	low-	598:601	arg1	LEPA					632:635	LEPA	632:635	LEPA	632:635	Sprague-Dawley rats were randomly divided into four groups for 8-week treatment with vehicle (control) and low-, medium-, and high-dose EPA (LEPA, MEPA, HEPA; 0, 75, 150, and 375 mg/kg/day, respectively).
25421533	0	46	theme	Hayata	67:72	arg1	rhizomes					74:81	Polygonatum Alte-lobatum Hayata rhizomes	42:81	Polygonatum Alte-lobatum Hayata rhizomes	42:81	Antioxidant and antifatigue activities of Polygonatum Alte-lobatum Hayata rhizomes in rats.
25421533	8	47	theme	total	1210:1214	arg1	ability					1228:1234	total antioxidant ability	1210:1234	total antioxidant ability of blood	1210:1243	EPA treatment dose-dependently increased endurance running time to exhaustion and superoxide dismutase activity and total antioxidant ability of blood.
25421533	4	48	dep	LEPA	632:635	arg1	mg/kg/day					670:678	0, 75, 150, and 375 mg/kg/day	650:678	0, 75, 150, and 375 mg/kg/day	650:678	Sprague-Dawley rats were randomly divided into four groups for 8-week treatment with vehicle (control) and low-, medium-, and high-dose EPA (LEPA, MEPA, HEPA; 0, 75, 150, and 375 mg/kg/day, respectively).
25421533	0	49	theme	Alte-lobatum	54:65	arg1	rhizomes					74:81	Polygonatum Alte-lobatum Hayata rhizomes	42:81	Polygonatum Alte-lobatum Hayata rhizomes	42:81	Antioxidant and antifatigue activities of Polygonatum Alte-lobatum Hayata rhizomes in rats.
25421533	11	50	theme	anti-fatigue	1494:1505	arg1	function					1523:1530	an anti-fatigue pharmacological function	1491:1530	an anti-fatigue pharmacological function	1491:1530	EPA could be a potential agent with an anti-fatigue pharmacological function.
25421533	1	51	theme	rhizomatous	127:137	arg1	herb					149:152	a rhizomatous perennial herb	125:152	a rhizomatous perennial herb	125:152	Polygonatum alte-lobatum Hayata, a rhizomatous perennial herb, belongs to the Liliaceae family and is endemic to Taiwan.
25421533	1	51	theme	rhizomatous	127:137	arg1	Polygonatum					92:102	Polygonatum alte-lobatum Hayata	92:122	Polygonatum alte-lobatum Hayata	92:122	Polygonatum alte-lobatum Hayata, a rhizomatous perennial herb, belongs to the Liliaceae family and is endemic to Taiwan.
25421533	11	52	theme	pharmacological	1507:1521	arg1	function					1523:1530	an anti-fatigue pharmacological function	1491:1530	an anti-fatigue pharmacological function	1491:1530	EPA could be a potential agent with an anti-fatigue pharmacological function.
25421533	8	53	theme	EPA	1094:1096	arg1	treatment					1098:1106	EPA treatment	1094:1106	EPA treatment	1094:1106	EPA treatment dose-dependently increased endurance running time to exhaustion and superoxide dismutase activity and total antioxidant ability of blood.
25421533	10	54	theme	glycogen	1348:1355	arg1	source					1386:1391	an important energy source	1366:1391	an important energy source for exercise	1366:1404	Hepatic glycogen content, an important energy source for exercise, was significantly increased with EPA treatment.
25421533	10	54	theme	glycogen	1348:1355	arg1	content					1357:1363	Hepatic glycogen content	1340:1363	Hepatic glycogen content	1340:1363	Hepatic glycogen content, an important energy source for exercise, was significantly increased with EPA treatment.
25421533	1	55	theme	perennial	139:147	arg1	herb					149:152	a rhizomatous perennial herb	125:152	a rhizomatous perennial herb	125:152	Polygonatum alte-lobatum Hayata, a rhizomatous perennial herb, belongs to the Liliaceae family and is endemic to Taiwan.
25421533	1	55	theme	perennial	139:147	arg1	Polygonatum					92:102	Polygonatum alte-lobatum Hayata	92:122	Polygonatum alte-lobatum Hayata	92:122	Polygonatum alte-lobatum Hayata, a rhizomatous perennial herb, belongs to the Liliaceae family and is endemic to Taiwan.
25421533	6	56	dep	content	939:945	arg1	times					959:963	times	959:963	times	959:963	EPA contained polyphenols, flavonoids and polysaccharides, with polysaccharide content at least 26 times greater than that of polyphenols and flavonoids.
25421533	9	57	theme	malondialdehyde	1301:1315	arg1	levels					1317:1322	serum urea nitrogen and malondialdehyde levels	1277:1322	levels	1317:1322	EPA dose-dependently decreased serum urea nitrogen and malondialdehyde levels after exercise.
25421533	3	58	theme	flavonoids	334:343	arg1	Levels					311:316	Levels	311:316	Levels of polyphenols, flavonoids and polysaccharides	311:363	Levels of polyphenols, flavonoids and polysaccharides and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free-radical scavenging activity were measured in extracts of P. alte-lobatum (EPA).
25421533	3	58	theme	flavonoids	334:343	arg1	activity					430:437	2,2-diphenyl-1-picrylhydrazyl (DPPH) free-radical scavenging activity	369:437	2,2-diphenyl-1-picrylhydrazyl (DPPH) free-radical scavenging activity	369:437	Levels of polyphenols, flavonoids and polysaccharides and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free-radical scavenging activity were measured in extracts of P. alte-lobatum (EPA).
25421533	9	59	theme	urea	1283:1286	arg1	nitrogen					1288:1295	serum urea nitrogen and malondialdehyde levels	1277:1322	nitrogen	1288:1295	EPA dose-dependently decreased serum urea nitrogen and malondialdehyde levels after exercise.
25421533	2	60	theme	anti-fatigue	249:260	arg1	activities					262:271	the antioxidant and anti-fatigue activities	229:271	the antioxidant and anti-fatigue activities of P. alte-lobatum in exercised rats	229:308	We investigated the antioxidant and anti-fatigue activities of P. alte-lobatum in exercised rats.
25421533	6	61	theme	26	956:957	arg1	times					959:963	times	959:963	times	959:963	EPA contained polyphenols, flavonoids and polysaccharides, with polysaccharide content at least 26 times greater than that of polyphenols and flavonoids.
25421533	0	62	theme	rhizomes	74:81	arg1	activities					28:37	Antioxidant and antifatigue activities	0:37	Antioxidant and antifatigue activities of Polygonatum Alte-lobatum Hayata rhizomes in rats.	0:90	Antioxidant and antifatigue activities of Polygonatum Alte-lobatum Hayata rhizomes in rats.
25421533	0	63	from	activities	28:37	arg1	rats					86:89	rats	86:89	rats	86:89	Antioxidant and antifatigue activities of Polygonatum Alte-lobatum Hayata rhizomes in rats.
25421533	8	64	theme	dismutase	1187:1195	arg1	activity					1197:1204	superoxide dismutase activity	1176:1204	superoxide dismutase activity	1176:1204	EPA treatment dose-dependently increased endurance running time to exhaustion and superoxide dismutase activity and total antioxidant ability of blood.
25421533	10	65	theme	energy	1379:1384	arg1	source					1386:1391	an important energy source	1366:1391	an important energy source for exercise	1366:1404	Hepatic glycogen content, an important energy source for exercise, was significantly increased with EPA treatment.
25421533	10	65	theme	energy	1379:1384	arg1	content					1357:1363	Hepatic glycogen content	1340:1363	Hepatic glycogen content	1340:1363	Hepatic glycogen content, an important energy source for exercise, was significantly increased with EPA treatment.
25421533	8	66	theme	superoxide	1176:1185	arg1	dismutase					1187:1195	superoxide dismutase	1176:1195	superoxide dismutase activity	1176:1204	EPA treatment dose-dependently increased endurance running time to exhaustion and superoxide dismutase activity and total antioxidant ability of blood.
25421533	4	67	theme	high-dose	617:625	arg1	EPA					627:629	high-dose EPA	617:629	high-dose EPA	617:629	Sprague-Dawley rats were randomly divided into four groups for 8-week treatment with vehicle (control) and low-, medium-, and high-dose EPA (LEPA, MEPA, HEPA; 0, 75, 150, and 375 mg/kg/day, respectively).
24641584	0	0	theme	hemicellulose	85:97	arg1	biosynthesis					114:125	hemicellulose glucuronoxylan biosynthesis	85:125	hemicellulose glucuronoxylan biosynthesis	85:125	Two cotton fiber-associated glycosyltransferases, GhGT43A1 and GhGT43C1, function in hemicellulose glucuronoxylan biosynthesis during plant development.
24641584	3	1	theme	molecular	346:354	arg1	sizes					356:360	the molecular sizes	342:360	Not only the amounts but also the molecular sizes of the hemicellulosic polysaccharides	312:398	Not only the amounts but also the molecular sizes of the hemicellulosic polysaccharides show substantial changes during cotton fiber development.
24641584	10	2	theme	xylan	1486:1490	arg1	synthesis					1492:1500	xylan synthesis	1486:1500	xylan synthesis	1486:1500	These findings suggest that GhGT43A1 and GhGT43C1 likely participate in xylan synthesis during fiber development.
24641584	1	3	theme	hemicellulosic	172:185	arg1	Xylan					153:157	Xylan	153:157	Xylan	153:157	Xylan is the major hemicellulosic constituent in dicot secondary cell walls.
24641584	1	3	theme	hemicellulosic	172:185	arg1	constituent					187:197	the major hemicellulosic constituent	162:197	the major hemicellulosic constituent in dicot secondary cell walls	162:227	Xylan is the major hemicellulosic constituent in dicot secondary cell walls.
24641584	6	4	theme	20 days	918:924	arg1	fiber					953:957	15 and 20 days post-anthesis (dpa) cotton fiber	911:957	fiber	953:957	We show that GhGT43A1 is highly and preferentially expressed in 15 and 20 days post-anthesis (dpa) cotton fiber, whereas GhGT43C1 is ubiquitously expressed in most organs, with especially high expression in 15 dpa fiber and hypocotyl.
24641584	6	5	theme	dpa	941:943	arg1	fiber					953:957	15 and 20 days post-anthesis (dpa) cotton fiber	911:957	fiber	953:957	We show that GhGT43A1 is highly and preferentially expressed in 15 and 20 days post-anthesis (dpa) cotton fiber, whereas GhGT43C1 is ubiquitously expressed in most organs, with especially high expression in 15 dpa fiber and hypocotyl.
24641584	5	6	theme	CAZy	761:764	arg1	family					771:776	the CAZy GT43 family	757:776	the CAZy GT43 family	757:776	In this study, we applied a bioinformatics approach and identified two putative GTs from cotton, designated GhGT43A1 and GhGT43C1, which belong to the CAZy GT43 family and are closely related to Arabidopsis IRX9 and IRX14, respectively.
24641584	6	7	theme	post-anthesis	926:938	arg1	fiber					953:957	15 and 20 days post-anthesis (dpa) cotton fiber	911:957	fiber	953:957	We show that GhGT43A1 is highly and preferentially expressed in 15 and 20 days post-anthesis (dpa) cotton fiber, whereas GhGT43C1 is ubiquitously expressed in most organs, with especially high expression in 15 dpa fiber and hypocotyl.
24641584	6	8	from	expression	1040:1049	arg1	hypocotyl					1071:1079	hypocotyl	1071:1079	hypocotyl	1071:1079	We show that GhGT43A1 is highly and preferentially expressed in 15 and 20 days post-anthesis (dpa) cotton fiber, whereas GhGT43C1 is ubiquitously expressed in most organs, with especially high expression in 15 dpa fiber and hypocotyl.
24641584	6	8	from	expression	1040:1049	arg1	fiber					1061:1065	15 dpa fiber	1054:1065	15 dpa fiber	1054:1065	We show that GhGT43A1 is highly and preferentially expressed in 15 and 20 days post-anthesis (dpa) cotton fiber, whereas GhGT43C1 is ubiquitously expressed in most organs, with especially high expression in 15 dpa fiber and hypocotyl.
24641584	1	9	from	constituent	187:197	arg1	walls					223:227	dicot secondary cell walls	202:227	dicot secondary cell walls	202:227	Xylan is the major hemicellulosic constituent in dicot secondary cell walls.
24641584	6	10	theme	most	1006:1009	arg1	organs					1011:1016	most organs	1006:1016	most organs	1006:1016	We show that GhGT43A1 is highly and preferentially expressed in 15 and 20 days post-anthesis (dpa) cotton fiber, whereas GhGT43C1 is ubiquitously expressed in most organs, with especially high expression in 15 dpa fiber and hypocotyl.
24641584	3	11	theme	substantial	405:415	arg1	changes					417:423	substantial changes	405:423	substantial changes	405:423	Not only the amounts but also the molecular sizes of the hemicellulosic polysaccharides show substantial changes during cotton fiber development.
24641584	4	12	theme	responsible	521:531	arg1	glycosyltransferases					494:513	glycosyltransferases	494:513	glycosyltransferases (GTs) responsible for synthesizing xylan	494:554	However, none of the genes encoding glycosyltransferases (GTs) responsible for synthesizing xylan have been isolated and characterized in cotton fiber.
24641584	4	12	theme	responsible	521:531	arg1	GTs					516:518	GTs	516:518	GTs	516:518	However, none of the genes encoding glycosyltransferases (GTs) responsible for synthesizing xylan have been isolated and characterized in cotton fiber.
24641584	3	13	theme	hemicellulosic	369:382	arg1	polysaccharides					384:398	the hemicellulosic polysaccharides	365:398	the hemicellulosic polysaccharides	365:398	Not only the amounts but also the molecular sizes of the hemicellulosic polysaccharides show substantial changes during cotton fiber development.
24641584	8	14	theme	GhGT43C1	1261:1268	arg1	overexpression					1231:1244	overexpression	1231:1244	overexpression of GhGT43A1 or GhGT43C1 in Arabidopsis	1231:1283	Furthermore, we show that overexpression of GhGT43A1 or GhGT43C1 in Arabidopsis results in increased xylan content.
24641584	3	15	theme	polysaccharides	384:398	arg1	polysaccharides					384:398	the hemicellulosic polysaccharides	365:398	the hemicellulosic polysaccharides	365:398	Not only the amounts but also the molecular sizes of the hemicellulosic polysaccharides show substantial changes during cotton fiber development.
24641584	3	15	theme	polysaccharides	384:398	arg1	sizes					356:360	the molecular sizes	342:360	Not only the amounts but also the molecular sizes of the hemicellulosic polysaccharides	312:398	Not only the amounts but also the molecular sizes of the hemicellulosic polysaccharides show substantial changes during cotton fiber development.
24641584	3	15	theme	polysaccharides	384:398	arg1	amounts					325:331	the amounts	321:331	Not only the amounts but also the molecular sizes of the hemicellulosic polysaccharides	312:398	Not only the amounts but also the molecular sizes of the hemicellulosic polysaccharides show substantial changes during cotton fiber development.
24641584	6	16	theme	15	911:912	arg1	fiber					953:957	15 and 20 days post-anthesis (dpa) cotton fiber	911:957	fiber	953:957	We show that GhGT43A1 is highly and preferentially expressed in 15 and 20 days post-anthesis (dpa) cotton fiber, whereas GhGT43C1 is ubiquitously expressed in most organs, with especially high expression in 15 dpa fiber and hypocotyl.
24641584	7	17	theme	IRX9	1175:1178	arg1	GhGT43C1					1137:1144	GhGT43C1	1137:1144	GhGT43C1	1137:1144	Complementation analysis demonstrates that GhG43A1 and GhGT43C1 are orthologs of Arabidopsis IRX9 and IRX14, respectively.
24641584	7	17	theme	IRX9	1175:1178	arg1	GhG43A1					1125:1131	GhG43A1	1125:1131	GhG43A1	1125:1131	Complementation analysis demonstrates that GhG43A1 and GhGT43C1 are orthologs of Arabidopsis IRX9 and IRX14, respectively.
24641584	7	17	theme	IRX9	1175:1178	arg1	orthologs					1150:1158	orthologs	1150:1158	orthologs of Arabidopsis IRX9 and IRX14	1150:1188	Complementation analysis demonstrates that GhG43A1 and GhGT43C1 are orthologs of Arabidopsis IRX9 and IRX14, respectively.
24641584	1	18	theme	dicot	202:206	arg1	walls					223:227	dicot secondary cell walls	202:227	dicot secondary cell walls	202:227	Xylan is the major hemicellulosic constituent in dicot secondary cell walls.
24641584	3	19	theme	cotton	432:437	arg1	development					445:455	cotton fiber development	432:455	cotton fiber development	432:455	Not only the amounts but also the molecular sizes of the hemicellulosic polysaccharides show substantial changes during cotton fiber development.
24641584	5	20	theme	putative	681:688	arg1	GTs					690:692	two putative GTs	677:692	two putative GTs	677:692	In this study, we applied a bioinformatics approach and identified two putative GTs from cotton, designated GhGT43A1 and GhGT43C1, which belong to the CAZy GT43 family and are closely related to Arabidopsis IRX9 and IRX14, respectively.
24641584	0	21	theme	plant	134:138	arg1	development					140:150	plant development	134:150	plant development	134:150	Two cotton fiber-associated glycosyltransferases, GhGT43A1 and GhGT43C1, function in hemicellulose glucuronoxylan biosynthesis during plant development.
24641584	1	22	theme	secondary	208:216	arg1	walls					223:227	dicot secondary cell walls	202:227	dicot secondary cell walls	202:227	Xylan is the major hemicellulosic constituent in dicot secondary cell walls.
24641584	3	23	theme	fiber	439:443	arg1	development					445:455	cotton fiber development	432:455	cotton fiber development	432:455	Not only the amounts but also the molecular sizes of the hemicellulosic polysaccharides show substantial changes during cotton fiber development.
24641584	9	24	theme	GhGT43A1	1357:1364	arg1	overexpression					1339:1352	overexpression	1339:1352	overexpression of GhGT43A1 or GhGT43C1	1339:1376	We also show that overexpression of GhGT43A1 or GhGT43C1 leads to more cellulose deposition.
24641584	9	25	theme	cellulose	1392:1400	arg1	deposition					1402:1411	more cellulose deposition	1387:1411	more cellulose deposition	1387:1411	We also show that overexpression of GhGT43A1 or GhGT43C1 leads to more cellulose deposition.
24641584	1	26	theme	cell	218:221	arg1	walls					223:227	dicot secondary cell walls	202:227	dicot secondary cell walls	202:227	Xylan is the major hemicellulosic constituent in dicot secondary cell walls.
24641584	7	27	theme	IRX14	1184:1188	arg1	GhGT43C1					1137:1144	GhGT43C1	1137:1144	GhGT43C1	1137:1144	Complementation analysis demonstrates that GhG43A1 and GhGT43C1 are orthologs of Arabidopsis IRX9 and IRX14, respectively.
24641584	7	27	theme	IRX14	1184:1188	arg1	GhG43A1					1125:1131	GhG43A1	1125:1131	GhG43A1	1125:1131	Complementation analysis demonstrates that GhG43A1 and GhGT43C1 are orthologs of Arabidopsis IRX9 and IRX14, respectively.
24641584	7	27	theme	IRX14	1184:1188	arg1	orthologs					1150:1158	orthologs	1150:1158	orthologs of Arabidopsis IRX9 and IRX14	1150:1188	Complementation analysis demonstrates that GhG43A1 and GhGT43C1 are orthologs of Arabidopsis IRX9 and IRX14, respectively.
24641584	0	28	theme	fiber-associated	11:26	arg1	GhGT43A1					50:57	GhGT43A1	50:57	GhGT43A1	50:57	Two cotton fiber-associated glycosyltransferases, GhGT43A1 and GhGT43C1, function in hemicellulose glucuronoxylan biosynthesis during plant development.
24641584	0	28	theme	fiber-associated	11:26	arg1	glycosyltransferases					28:47	Two cotton fiber-associated glycosyltransferases	0:47	Two cotton fiber-associated glycosyltransferases	0:47	Two cotton fiber-associated glycosyltransferases, GhGT43A1 and GhGT43C1, function in hemicellulose glucuronoxylan biosynthesis during plant development.
24641584	0	28	theme	fiber-associated	11:26	arg1	GhGT43C1					63:70	GhGT43C1	63:70	GhGT43C1	63:70	Two cotton fiber-associated glycosyltransferases, GhGT43A1 and GhGT43C1, function in hemicellulose glucuronoxylan biosynthesis during plant development.
24641584	9	29	theme	GhGT43C1	1369:1376	arg1	overexpression					1339:1352	overexpression	1339:1352	overexpression of GhGT43A1 or GhGT43C1	1339:1376	We also show that overexpression of GhGT43A1 or GhGT43C1 leads to more cellulose deposition.
24641584	7	30	theme	Complementation	1082:1096	arg1	analysis					1098:1105	Complementation analysis	1082:1105	Complementation analysis	1082:1105	Complementation analysis demonstrates that GhG43A1 and GhGT43C1 are orthologs of Arabidopsis IRX9 and IRX14, respectively.
24641584	6	31	theme	dpa	1057:1059	arg1	fiber					1061:1065	15 dpa fiber	1054:1065	15 dpa fiber	1054:1065	We show that GhGT43A1 is highly and preferentially expressed in 15 and 20 days post-anthesis (dpa) cotton fiber, whereas GhGT43C1 is ubiquitously expressed in most organs, with especially high expression in 15 dpa fiber and hypocotyl.
24641584	5	32	theme	Arabidopsis	805:815	arg1	IRX9					817:820	Arabidopsis IRX9	805:820	Arabidopsis IRX9	805:820	In this study, we applied a bioinformatics approach and identified two putative GTs from cotton, designated GhGT43A1 and GhGT43C1, which belong to the CAZy GT43 family and are closely related to Arabidopsis IRX9 and IRX14, respectively.
24641584	8	33	theme	xylan	1306:1310	arg1	content					1312:1318	increased xylan content	1296:1318	increased xylan content	1296:1318	Furthermore, we show that overexpression of GhGT43A1 or GhGT43C1 in Arabidopsis results in increased xylan content.
24641584	2	34	theme	fiber	262:266	arg1	composition					240:250	Cell wall composition	230:250	Cell wall composition of cotton fiber	230:266	Cell wall composition of cotton fiber changes dynamically throughout development.
24641584	6	35	theme	15	1054:1055	arg1	dpa					1057:1059	dpa	1057:1059	dpa	1057:1059	We show that GhGT43A1 is highly and preferentially expressed in 15 and 20 days post-anthesis (dpa) cotton fiber, whereas GhGT43C1 is ubiquitously expressed in most organs, with especially high expression in 15 dpa fiber and hypocotyl.
24641584	5	36	theme	GT43	766:769	arg1	family					771:776	the CAZy GT43 family	757:776	the CAZy GT43 family	757:776	In this study, we applied a bioinformatics approach and identified two putative GTs from cotton, designated GhGT43A1 and GhGT43C1, which belong to the CAZy GT43 family and are closely related to Arabidopsis IRX9 and IRX14, respectively.
24641584	2	37	theme	cotton	255:260	arg1	fiber					262:266	cotton fiber	255:266	cotton fiber	255:266	Cell wall composition of cotton fiber changes dynamically throughout development.
24641584	6	38	theme	cotton	946:951	arg1	fiber					953:957	15 and 20 days post-anthesis (dpa) cotton fiber	911:957	fiber	953:957	We show that GhGT43A1 is highly and preferentially expressed in 15 and 20 days post-anthesis (dpa) cotton fiber, whereas GhGT43C1 is ubiquitously expressed in most organs, with especially high expression in 15 dpa fiber and hypocotyl.
24641584	8	39	theme	GhGT43A1	1249:1256	arg1	overexpression					1231:1244	overexpression	1231:1244	overexpression of GhGT43A1 or GhGT43C1 in Arabidopsis	1231:1283	Furthermore, we show that overexpression of GhGT43A1 or GhGT43C1 in Arabidopsis results in increased xylan content.
24641584	8	40	from	overexpression	1231:1244	arg1	Arabidopsis					1273:1283	Arabidopsis	1273:1283	Arabidopsis	1273:1283	Furthermore, we show that overexpression of GhGT43A1 or GhGT43C1 in Arabidopsis results in increased xylan content.
24641584	7	41	theme	Arabidopsis	1163:1173	arg1	IRX9					1175:1178	Arabidopsis IRX9	1163:1178	Arabidopsis IRX9	1163:1178	Complementation analysis demonstrates that GhG43A1 and GhGT43C1 are orthologs of Arabidopsis IRX9 and IRX14, respectively.
24641584	4	42	theme	cotton	596:601	arg1	fiber					603:607	cotton fiber	596:607	cotton fiber	596:607	However, none of the genes encoding glycosyltransferases (GTs) responsible for synthesizing xylan have been isolated and characterized in cotton fiber.
24641584	2	43	theme	wall	235:238	arg1	composition					240:250	Cell wall composition	230:250	Cell wall composition of cotton fiber	230:266	Cell wall composition of cotton fiber changes dynamically throughout development.
24641584	10	44	theme	fiber	1509:1513	arg1	development					1515:1525	fiber development	1509:1525	fiber development	1509:1525	These findings suggest that GhGT43A1 and GhGT43C1 likely participate in xylan synthesis during fiber development.
24641584	5	45	from	cotton	699:704	arg1	GTs					690:692	two putative GTs	677:692	two putative GTs	677:692	In this study, we applied a bioinformatics approach and identified two putative GTs from cotton, designated GhGT43A1 and GhGT43C1, which belong to the CAZy GT43 family and are closely related to Arabidopsis IRX9 and IRX14, respectively.
24641584	2	46	theme	Cell	230:233	arg1	composition					240:250	Cell wall composition	230:250	Cell wall composition of cotton fiber	230:266	Cell wall composition of cotton fiber changes dynamically throughout development.
24641584	5	47	theme	bioinformatics	638:651	arg1	approach					653:660	a bioinformatics approach	636:660	a bioinformatics approach	636:660	In this study, we applied a bioinformatics approach and identified two putative GTs from cotton, designated GhGT43A1 and GhGT43C1, which belong to the CAZy GT43 family and are closely related to Arabidopsis IRX9 and IRX14, respectively.
24641584	1	48	theme	major	166:170	arg1	Xylan					153:157	Xylan	153:157	Xylan	153:157	Xylan is the major hemicellulosic constituent in dicot secondary cell walls.
24641584	1	48	theme	major	166:170	arg1	constituent					187:197	the major hemicellulosic constituent	162:197	the major hemicellulosic constituent in dicot secondary cell walls	162:227	Xylan is the major hemicellulosic constituent in dicot secondary cell walls.
24641584	6	49	theme	high	1035:1038	arg1	expression					1040:1049	especially high expression	1024:1049	especially high expression in 15 dpa fiber and hypocotyl	1024:1079	We show that GhGT43A1 is highly and preferentially expressed in 15 and 20 days post-anthesis (dpa) cotton fiber, whereas GhGT43C1 is ubiquitously expressed in most organs, with especially high expression in 15 dpa fiber and hypocotyl.
24641584	8	50	theme	increased	1296:1304	arg1	content					1312:1318	increased xylan content	1296:1318	increased xylan content	1296:1318	Furthermore, we show that overexpression of GhGT43A1 or GhGT43C1 in Arabidopsis results in increased xylan content.
24641584	6	51	dep	expressed	898:906	arg1	whereas					960:966	whereas	960:966	whereas	960:966	We show that GhGT43A1 is highly and preferentially expressed in 15 and 20 days post-anthesis (dpa) cotton fiber, whereas GhGT43C1 is ubiquitously expressed in most organs, with especially high expression in 15 dpa fiber and hypocotyl.
24641584	0	52	theme	cotton	4:9	arg1	GhGT43A1					50:57	GhGT43A1	50:57	GhGT43A1	50:57	Two cotton fiber-associated glycosyltransferases, GhGT43A1 and GhGT43C1, function in hemicellulose glucuronoxylan biosynthesis during plant development.
24641584	0	52	theme	cotton	4:9	arg1	glycosyltransferases					28:47	Two cotton fiber-associated glycosyltransferases	0:47	Two cotton fiber-associated glycosyltransferases	0:47	Two cotton fiber-associated glycosyltransferases, GhGT43A1 and GhGT43C1, function in hemicellulose glucuronoxylan biosynthesis during plant development.
24641584	0	52	theme	cotton	4:9	arg1	GhGT43C1					63:70	GhGT43C1	63:70	GhGT43C1	63:70	Two cotton fiber-associated glycosyltransferases, GhGT43A1 and GhGT43C1, function in hemicellulose glucuronoxylan biosynthesis during plant development.
24641584	0	53	theme	glucuronoxylan	99:112	arg1	biosynthesis					114:125	hemicellulose glucuronoxylan biosynthesis	85:125	hemicellulose glucuronoxylan biosynthesis	85:125	Two cotton fiber-associated glycosyltransferases, GhGT43A1 and GhGT43C1, function in hemicellulose glucuronoxylan biosynthesis during plant development.
26874335	0	0	theme	-amino	85:90	arg1	derivatives					97:107	fluorinated cyclic β(3) -amino acid derivatives	61:107	fluorinated cyclic β(3) -amino acid derivatives	61:107	High-performance liquid chromatographic enantioseparation of fluorinated cyclic β(3) -amino acid derivatives on polysaccharide-based chiral stationary phases.
26874335	3	1	theme	temperature	788:798	arg1	range					800:804	the temperature range 5-40 °C	784:812	the temperature range 5-40 °C	784:812	The enantioseparations were carried out in normal-phase mode with n-hexane/alcohol/alkylamine mobile phases in the temperature range 5-40 °C.
26874335	1	2	theme	nonfluorinated	175:188	arg1	counterparts					190:201	nonfluorinated counterparts	175:201	nonfluorinated counterparts	175:201	Comparison with nonfluorinated counterparts.
26874335	2	3	theme	3,5-dimethylphenyl	590:607	arg1	tris-					584:588	amylose tris-	576:588	amylose tris-(3,5-dimethylphenyl carbamate)	576:618	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	3	theme	3,5-dimethylphenyl	590:607	arg1	carbamate					609:617	3,5-dimethylphenyl carbamate	590:617	3,5-dimethylphenyl carbamate	590:617	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	4	theme	cellulose	390:398	arg1	tris-					400:404	cellulose tris-	390:404	cellulose tris-(3,5-dimethylphenyl carbamate)	390:434	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	4	theme	cellulose	390:398	arg1	tris-					499:503	cellulose tris-	489:503	cellulose tris-(4-methylbenzoate)	489:521	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	4	theme	cellulose	390:398	arg1	tris-					534:538	cellulose tris-	524:538	cellulose tris-(4-chloro-3-methylphenyl carbamate)	524:573	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	4	theme	cellulose	390:398	arg1	tris-					447:451	cellulose tris-	437:451	cellulose tris-(3-chloro-4-methylphenyl carbamate)	437:486	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	4	theme	cellulose	390:398	arg1	tris-					631:635	amylose tris-	623:635	amylose tris-(5-chloro-2-methylphenyl carbamate)	623:670	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	4	theme	cellulose	390:398	arg1	tris-					584:588	amylose tris-	576:588	amylose tris-(3,5-dimethylphenyl carbamate)	576:618	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	4	theme	cellulose	390:398	arg1	carbamate					425:433	3,5-dimethylphenyl carbamate	406:433	3,5-dimethylphenyl carbamate	406:433	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	4	theme	cellulose	390:398	arg1	selectors					380:388	chiral selectors	373:388	chiral selectors	373:388	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	5	5	theme	α	1073:1073	arg1	plots					1061:1065	plots	1061:1065	plots of ln α vs. 1/T	1061:1081	Thermodynamic parameters were calculated from plots of ln α vs. 1/T.
26874335	0	6	theme	derivatives	97:107	arg1	enantioseparation					40:56	High-performance liquid chromatographic enantioseparation	0:56	High-performance liquid chromatographic enantioseparation of fluorinated cyclic β(3) -amino acid derivatives on polysaccharide-based chiral stationary phases.	0:157	High-performance liquid chromatographic enantioseparation of fluorinated cyclic β(3) -amino acid derivatives on polysaccharide-based chiral stationary phases.
26874335	5	7	theme	1/T	1079:1081	arg1	plots					1061:1065	plots	1061:1065	plots of ln α vs. 1/T	1061:1081	Thermodynamic parameters were calculated from plots of ln α vs. 1/T.
26874335	1	8	with	Comparison	159:168	arg1	counterparts					190:201	nonfluorinated counterparts	175:201	nonfluorinated counterparts	175:201	Comparison with nonfluorinated counterparts.
26874335	4	9	theme	composition	847:857	arg1	effects					819:825	The effects	815:825	The effects of the mobile phase composition, the nature and concentration of the alcohol and alkylamine additives, the structures of the analytes and temperature on the separations	815:994	The effects of the mobile phase composition, the nature and concentration of the alcohol and alkylamine additives, the structures of the analytes and temperature on the separations were investigated.
26874335	0	10	theme	acid	92:95	arg1	derivatives					97:107	fluorinated cyclic β(3) -amino acid derivatives	61:107	fluorinated cyclic β(3) -amino acid derivatives	61:107	High-performance liquid chromatographic enantioseparation of fluorinated cyclic β(3) -amino acid derivatives on polysaccharide-based chiral stationary phases.
26874335	4	11	theme	analytes	952:959	arg1	concentration					875:887	concentration	875:887	concentration	875:887	The effects of the mobile phase composition, the nature and concentration of the alcohol and alkylamine additives, the structures of the analytes and temperature on the separations were investigated.
26874335	4	11	theme	analytes	952:959	arg1	composition					847:857	the mobile phase composition	830:857	the mobile phase composition	830:857	The effects of the mobile phase composition, the nature and concentration of the alcohol and alkylamine additives, the structures of the analytes and temperature on the separations were investigated.
26874335	4	11	theme	analytes	952:959	arg1	temperature					965:975	temperature	965:975	temperature	965:975	The effects of the mobile phase composition, the nature and concentration of the alcohol and alkylamine additives, the structures of the analytes and temperature on the separations were investigated.
26874335	4	11	theme	analytes	952:959	arg1	structures					934:943	the structures	930:943	the structures of the analytes	930:959	The effects of the mobile phase composition, the nature and concentration of the alcohol and alkylamine additives, the structures of the analytes and temperature on the separations were investigated.
26874335	4	11	theme	analytes	952:959	arg1	nature					864:869	nature	864:869	nature	864:869	The effects of the mobile phase composition, the nature and concentration of the alcohol and alkylamine additives, the structures of the analytes and temperature on the separations were investigated.
26874335	4	12	theme	phase	841:845	arg1	composition					847:857	the mobile phase composition	830:857	the mobile phase composition	830:857	The effects of the mobile phase composition, the nature and concentration of the alcohol and alkylamine additives, the structures of the analytes and temperature on the separations were investigated.
26874335	3	13	with	mode	729:732	arg1	phases					774:779	n-hexane/alcohol/alkylamine mobile phases	739:779	n-hexane/alcohol/alkylamine mobile phases in the temperature range 5-40 °C	739:812	The enantioseparations were carried out in normal-phase mode with n-hexane/alcohol/alkylamine mobile phases in the temperature range 5-40 °C.
26874335	0	14	theme	polysaccharide-based	112:131	arg1	phases					151:156	polysaccharide-based chiral stationary phases	112:156	polysaccharide-based chiral stationary phases	112:156	High-performance liquid chromatographic enantioseparation of fluorinated cyclic β(3) -amino acid derivatives on polysaccharide-based chiral stationary phases.
26874335	2	15	theme	counterparts	303:314	arg1	stereoisomers					208:220	The stereoisomers	204:220	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts	204:314	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	16	theme	cellulose	437:445	arg1	tris-					400:404	cellulose tris-	390:404	cellulose tris-(3,5-dimethylphenyl carbamate)	390:434	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	16	theme	cellulose	437:445	arg1	tris-					499:503	cellulose tris-	489:503	cellulose tris-(4-methylbenzoate)	489:521	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	16	theme	cellulose	437:445	arg1	tris-					534:538	cellulose tris-	524:538	cellulose tris-(4-chloro-3-methylphenyl carbamate)	524:573	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	16	theme	cellulose	437:445	arg1	tris-					447:451	cellulose tris-	437:451	cellulose tris-(3-chloro-4-methylphenyl carbamate)	437:486	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	16	theme	cellulose	437:445	arg1	tris-					631:635	amylose tris-	623:635	amylose tris-(5-chloro-2-methylphenyl carbamate)	623:670	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	16	theme	cellulose	437:445	arg1	tris-					584:588	amylose tris-	576:588	amylose tris-(3,5-dimethylphenyl carbamate)	576:618	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	16	theme	cellulose	437:445	arg1	carbamate					477:485	3-chloro-4-methylphenyl carbamate	453:485	3-chloro-4-methylphenyl carbamate	453:485	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	16	theme	cellulose	437:445	arg1	selectors					380:388	chiral selectors	373:388	chiral selectors	373:388	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	7	17	theme	retention	1247:1255	arg1	factor					1257:1262	the retention factor	1243:1262	the retention factor	1243:1262	The enantioseparation was enthalpically controlled, the retention factor and the separation factor decreasing with increasing temperature, but entropically controlled separation was also observed.
26874335	8	18	theme	elution	1392:1398	arg1	sequence					1400:1407	The elution sequence	1388:1407	The elution sequence	1388:1407	The elution sequence was determined for all of the investigated analytes.
26874335	3	19	from	phases	774:779	arg1	range					800:804	the temperature range 5-40 °C	784:812	the temperature range 5-40 °C	784:812	The enantioseparations were carried out in normal-phase mode with n-hexane/alcohol/alkylamine mobile phases in the temperature range 5-40 °C.
26874335	0	20	theme	High-performance	0:15	arg1	enantioseparation					40:56	High-performance liquid chromatographic enantioseparation	0:56	High-performance liquid chromatographic enantioseparation of fluorinated cyclic β(3) -amino acid derivatives on polysaccharide-based chiral stationary phases.	0:157	High-performance liquid chromatographic enantioseparation of fluorinated cyclic β(3) -amino acid derivatives on polysaccharide-based chiral stationary phases.
26874335	0	21	theme	liquid	17:22	arg1	enantioseparation					40:56	High-performance liquid chromatographic enantioseparation	0:56	High-performance liquid chromatographic enantioseparation of fluorinated cyclic β(3) -amino acid derivatives on polysaccharide-based chiral stationary phases.	0:157	High-performance liquid chromatographic enantioseparation of fluorinated cyclic β(3) -amino acid derivatives on polysaccharide-based chiral stationary phases.
26874335	2	22	theme	cellulose	524:532	arg1	tris-					400:404	cellulose tris-	390:404	cellulose tris-(3,5-dimethylphenyl carbamate)	390:434	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	22	theme	cellulose	524:532	arg1	tris-					499:503	cellulose tris-	489:503	cellulose tris-(4-methylbenzoate)	489:521	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	22	theme	cellulose	524:532	arg1	tris-					534:538	cellulose tris-	524:538	cellulose tris-(4-chloro-3-methylphenyl carbamate)	524:573	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	22	theme	cellulose	524:532	arg1	tris-					447:451	cellulose tris-	437:451	cellulose tris-(3-chloro-4-methylphenyl carbamate)	437:486	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	22	theme	cellulose	524:532	arg1	tris-					631:635	amylose tris-	623:635	amylose tris-(5-chloro-2-methylphenyl carbamate)	623:670	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	22	theme	cellulose	524:532	arg1	tris-					584:588	amylose tris-	576:588	amylose tris-(3,5-dimethylphenyl carbamate)	576:618	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	22	theme	cellulose	524:532	arg1	selectors					380:388	chiral selectors	373:388	chiral selectors	373:388	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	22	theme	cellulose	524:532	arg1	carbamate					564:572	4-chloro-3-methylphenyl carbamate	540:572	4-chloro-3-methylphenyl carbamate	540:572	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	4	23	theme	structures	934:943	arg1	effects					819:825	The effects	815:825	The effects of the mobile phase composition, the nature and concentration of the alcohol and alkylamine additives, the structures of the analytes and temperature on the separations	815:994	The effects of the mobile phase composition, the nature and concentration of the alcohol and alkylamine additives, the structures of the analytes and temperature on the separations were investigated.
26874335	0	24	from	enantioseparation	40:56	arg1	phases					151:156	polysaccharide-based chiral stationary phases	112:156	polysaccharide-based chiral stationary phases	112:156	High-performance liquid chromatographic enantioseparation of fluorinated cyclic β(3) -amino acid derivatives on polysaccharide-based chiral stationary phases.
26874335	8	25	theme	investigated	1439:1450	arg1	analytes					1452:1459	the investigated analytes	1435:1459	the investigated analytes	1435:1459	The elution sequence was determined for all of the investigated analytes.
26874335	4	26	theme	temperature	965:975	arg1	effects					819:825	The effects	815:825	The effects of the mobile phase composition, the nature and concentration of the alcohol and alkylamine additives, the structures of the analytes and temperature on the separations	815:994	The effects of the mobile phase composition, the nature and concentration of the alcohol and alkylamine additives, the structures of the analytes and temperature on the separations were investigated.
26874335	2	27	theme	cellulose	489:497	arg1	tris-					400:404	cellulose tris-	390:404	cellulose tris-(3,5-dimethylphenyl carbamate)	390:434	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	27	theme	cellulose	489:497	arg1	tris-					499:503	cellulose tris-	489:503	cellulose tris-(4-methylbenzoate)	489:521	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	27	theme	cellulose	489:497	arg1	tris-					534:538	cellulose tris-	524:538	cellulose tris-(4-chloro-3-methylphenyl carbamate)	524:573	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	27	theme	cellulose	489:497	arg1	tris-					447:451	cellulose tris-	437:451	cellulose tris-(3-chloro-4-methylphenyl carbamate)	437:486	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	27	theme	cellulose	489:497	arg1	tris-					631:635	amylose tris-	623:635	amylose tris-(5-chloro-2-methylphenyl carbamate)	623:670	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	27	theme	cellulose	489:497	arg1	tris-					584:588	amylose tris-	576:588	amylose tris-(3,5-dimethylphenyl carbamate)	576:618	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	27	theme	cellulose	489:497	arg1	4-methylbenzoate					505:520	4-methylbenzoate	505:520	4-methylbenzoate	505:520	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	27	theme	cellulose	489:497	arg1	selectors					380:388	chiral selectors	373:388	chiral selectors	373:388	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	9	28	dep	&	1490:1490	arg1	Sons					1492:1495	Sons	1492:1495	Sons	1492:1495	Copyright © 2016 John Wiley & Sons, Ltd.
26874335	2	29	theme	stationary	341:350	arg1	phases					352:357	chiral stationary phases	334:357	chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate)	334:670	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	4	30	dep	nature	864:869	arg1	the					860:862	the	860:862	the	860:862	The effects of the mobile phase composition, the nature and concentration of the alcohol and alkylamine additives, the structures of the analytes and temperature on the separations were investigated.
26874335	0	31	theme	stationary	140:149	arg1	phases					151:156	polysaccharide-based chiral stationary phases	112:156	polysaccharide-based chiral stationary phases	112:156	High-performance liquid chromatographic enantioseparation of fluorinated cyclic β(3) -amino acid derivatives on polysaccharide-based chiral stationary phases.
26874335	4	32	from	effects	819:825	arg1	separations					984:994	the separations	980:994	the separations	980:994	The effects of the mobile phase composition, the nature and concentration of the alcohol and alkylamine additives, the structures of the analytes and temperature on the separations were investigated.
26874335	2	33	theme	chiral	334:339	arg1	phases					352:357	chiral stationary phases	334:357	chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate)	334:670	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	4	34	theme	alcohol	896:902	arg1	additives					919:927	the alcohol and alkylamine additives	892:927	the alcohol and alkylamine additives	892:927	The effects of the mobile phase composition, the nature and concentration of the alcohol and alkylamine additives, the structures of the analytes and temperature on the separations were investigated.
26874335	0	35	theme	chromatographic	24:38	arg1	enantioseparation					40:56	High-performance liquid chromatographic enantioseparation	0:56	High-performance liquid chromatographic enantioseparation of fluorinated cyclic β(3) -amino acid derivatives on polysaccharide-based chiral stationary phases.	0:157	High-performance liquid chromatographic enantioseparation of fluorinated cyclic β(3) -amino acid derivatives on polysaccharide-based chiral stationary phases.
26874335	0	36	theme	chiral	133:138	arg1	phases					151:156	polysaccharide-based chiral stationary phases	112:156	polysaccharide-based chiral stationary phases	112:156	High-performance liquid chromatographic enantioseparation of fluorinated cyclic β(3) -amino acid derivatives on polysaccharide-based chiral stationary phases.
26874335	2	37	theme	3-chloro-4-methylphenyl	453:475	arg1	tris-					447:451	cellulose tris-	437:451	cellulose tris-(3-chloro-4-methylphenyl carbamate)	437:486	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	37	theme	3-chloro-4-methylphenyl	453:475	arg1	carbamate					477:485	3-chloro-4-methylphenyl carbamate	453:485	3-chloro-4-methylphenyl carbamate	453:485	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	0	38	theme	fluorinated	61:71	arg1	derivatives					97:107	fluorinated cyclic β(3) -amino acid derivatives	61:107	fluorinated cyclic β(3) -amino acid derivatives	61:107	High-performance liquid chromatographic enantioseparation of fluorinated cyclic β(3) -amino acid derivatives on polysaccharide-based chiral stationary phases.
26874335	2	39	theme	chiral	373:378	arg1	tris-					400:404	cellulose tris-	390:404	cellulose tris-(3,5-dimethylphenyl carbamate)	390:434	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	39	theme	chiral	373:378	arg1	tris-					499:503	cellulose tris-	489:503	cellulose tris-(4-methylbenzoate)	489:521	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	39	theme	chiral	373:378	arg1	tris-					534:538	cellulose tris-	524:538	cellulose tris-(4-chloro-3-methylphenyl carbamate)	524:573	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	39	theme	chiral	373:378	arg1	tris-					447:451	cellulose tris-	437:451	cellulose tris-(3-chloro-4-methylphenyl carbamate)	437:486	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	39	theme	chiral	373:378	arg1	tris-					631:635	amylose tris-	623:635	amylose tris-(5-chloro-2-methylphenyl carbamate)	623:670	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	39	theme	chiral	373:378	arg1	tris-					584:588	amylose tris-	576:588	amylose tris-(3,5-dimethylphenyl carbamate)	576:618	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	39	theme	chiral	373:378	arg1	selectors					380:388	chiral selectors	373:388	chiral selectors	373:388	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	3	40	theme	normal-phase	716:727	arg1	mode					729:732	normal-phase mode	716:732	normal-phase mode with n-hexane/alcohol/alkylamine mobile phases in the temperature range 5-40 °C	716:812	The enantioseparations were carried out in normal-phase mode with n-hexane/alcohol/alkylamine mobile phases in the temperature range 5-40 °C.
26874335	2	41	theme	-amino	254:259	arg1	derivatives					266:276	five fluorinated cyclic β(3) -amino acid derivatives	225:276	five fluorinated cyclic β(3) -amino acid derivatives	225:276	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	42	dep	derivatives	266:276	arg1	3					251:251	3	251:251	3	251:251	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	0	43	theme	β	80:80	arg1	derivatives					97:107	fluorinated cyclic β(3) -amino acid derivatives	61:107	fluorinated cyclic β(3) -amino acid derivatives	61:107	High-performance liquid chromatographic enantioseparation of fluorinated cyclic β(3) -amino acid derivatives on polysaccharide-based chiral stationary phases.
26874335	2	44	theme	amylose	576:582	arg1	selectors					380:388	chiral selectors	373:388	chiral selectors	373:388	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	44	theme	amylose	576:582	arg1	tris-					400:404	cellulose tris-	390:404	cellulose tris-(3,5-dimethylphenyl carbamate)	390:434	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	44	theme	amylose	576:582	arg1	tris-					499:503	cellulose tris-	489:503	cellulose tris-(4-methylbenzoate)	489:521	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	44	theme	amylose	576:582	arg1	tris-					534:538	cellulose tris-	524:538	cellulose tris-(4-chloro-3-methylphenyl carbamate)	524:573	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	44	theme	amylose	576:582	arg1	tris-					447:451	cellulose tris-	437:451	cellulose tris-(3-chloro-4-methylphenyl carbamate)	437:486	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	44	theme	amylose	576:582	arg1	tris-					631:635	amylose tris-	623:635	amylose tris-(5-chloro-2-methylphenyl carbamate)	623:670	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	44	theme	amylose	576:582	arg1	tris-					584:588	amylose tris-	576:588	amylose tris-(3,5-dimethylphenyl carbamate)	576:618	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	44	theme	amylose	576:582	arg1	carbamate					609:617	3,5-dimethylphenyl carbamate	590:617	3,5-dimethylphenyl carbamate	590:617	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	4	45	theme	alkylamine	908:917	arg1	additives					919:927	the alcohol and alkylamine additives	892:927	the alcohol and alkylamine additives	892:927	The effects of the mobile phase composition, the nature and concentration of the alcohol and alkylamine additives, the structures of the analytes and temperature on the separations were investigated.
26874335	7	46	theme	controlled	1347:1356	arg1	separation					1358:1367	entropically controlled separation	1334:1367	entropically controlled separation	1334:1367	The enantioseparation was enthalpically controlled, the retention factor and the separation factor decreasing with increasing temperature, but entropically controlled separation was also observed.
26874335	0	47	theme	cyclic	73:78	arg1	derivatives					97:107	fluorinated cyclic β(3) -amino acid derivatives	61:107	fluorinated cyclic β(3) -amino acid derivatives	61:107	High-performance liquid chromatographic enantioseparation of fluorinated cyclic β(3) -amino acid derivatives on polysaccharide-based chiral stationary phases.
26874335	4	48	theme	additives	919:927	arg1	concentration					875:887	concentration	875:887	concentration	875:887	The effects of the mobile phase composition, the nature and concentration of the alcohol and alkylamine additives, the structures of the analytes and temperature on the separations were investigated.
26874335	4	48	theme	additives	919:927	arg1	composition					847:857	the mobile phase composition	830:857	the mobile phase composition	830:857	The effects of the mobile phase composition, the nature and concentration of the alcohol and alkylamine additives, the structures of the analytes and temperature on the separations were investigated.
26874335	4	48	theme	additives	919:927	arg1	temperature					965:975	temperature	965:975	temperature	965:975	The effects of the mobile phase composition, the nature and concentration of the alcohol and alkylamine additives, the structures of the analytes and temperature on the separations were investigated.
26874335	4	48	theme	additives	919:927	arg1	structures					934:943	the structures	930:943	the structures of the analytes	930:959	The effects of the mobile phase composition, the nature and concentration of the alcohol and alkylamine additives, the structures of the analytes and temperature on the separations were investigated.
26874335	4	48	theme	additives	919:927	arg1	nature					864:869	nature	864:869	nature	864:869	The effects of the mobile phase composition, the nature and concentration of the alcohol and alkylamine additives, the structures of the analytes and temperature on the separations were investigated.
26874335	2	49	theme	amylose	623:629	arg1	carbamate					661:669	5-chloro-2-methylphenyl carbamate	637:669	5-chloro-2-methylphenyl carbamate	637:669	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	49	theme	amylose	623:629	arg1	tris-					400:404	cellulose tris-	390:404	cellulose tris-(3,5-dimethylphenyl carbamate)	390:434	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	49	theme	amylose	623:629	arg1	tris-					499:503	cellulose tris-	489:503	cellulose tris-(4-methylbenzoate)	489:521	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	49	theme	amylose	623:629	arg1	tris-					534:538	cellulose tris-	524:538	cellulose tris-(4-chloro-3-methylphenyl carbamate)	524:573	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	49	theme	amylose	623:629	arg1	tris-					447:451	cellulose tris-	437:451	cellulose tris-(3-chloro-4-methylphenyl carbamate)	437:486	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	49	theme	amylose	623:629	arg1	tris-					631:635	amylose tris-	623:635	amylose tris-(5-chloro-2-methylphenyl carbamate)	623:670	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	49	theme	amylose	623:629	arg1	tris-					584:588	amylose tris-	576:588	amylose tris-(3,5-dimethylphenyl carbamate)	576:618	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	49	theme	amylose	623:629	arg1	selectors					380:388	chiral selectors	373:388	chiral selectors	373:388	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	50	theme	β	249:249	arg1	derivatives					266:276	five fluorinated cyclic β(3) -amino acid derivatives	225:276	five fluorinated cyclic β(3) -amino acid derivatives	225:276	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	3	51	theme	mobile	767:772	arg1	phases					774:779	n-hexane/alcohol/alkylamine mobile phases	739:779	n-hexane/alcohol/alkylamine mobile phases in the temperature range 5-40 °C	739:812	The enantioseparations were carried out in normal-phase mode with n-hexane/alcohol/alkylamine mobile phases in the temperature range 5-40 °C.
26874335	2	52	theme	5-chloro-2-methylphenyl	637:659	arg1	carbamate					661:669	5-chloro-2-methylphenyl carbamate	637:669	5-chloro-2-methylphenyl carbamate	637:669	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	52	theme	5-chloro-2-methylphenyl	637:659	arg1	tris-					631:635	amylose tris-	623:635	amylose tris-(5-chloro-2-methylphenyl carbamate)	623:670	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	53	theme	cyclic	242:247	arg1	derivatives					266:276	five fluorinated cyclic β(3) -amino acid derivatives	225:276	five fluorinated cyclic β(3) -amino acid derivatives	225:276	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	4	54	theme	mobile	834:839	arg1	composition					847:857	the mobile phase composition	830:857	the mobile phase composition	830:857	The effects of the mobile phase composition, the nature and concentration of the alcohol and alkylamine additives, the structures of the analytes and temperature on the separations were investigated.
26874335	2	55	theme	nonfluorinated	288:301	arg1	counterparts					303:314	their nonfluorinated counterparts	282:314	their nonfluorinated counterparts	282:314	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	56	theme	fluorinated	230:240	arg1	derivatives					266:276	five fluorinated cyclic β(3) -amino acid derivatives	225:276	five fluorinated cyclic β(3) -amino acid derivatives	225:276	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	4	57	theme	nature	864:869	arg1	effects					819:825	The effects	815:825	The effects of the mobile phase composition, the nature and concentration of the alcohol and alkylamine additives, the structures of the analytes and temperature on the separations	815:994	The effects of the mobile phase composition, the nature and concentration of the alcohol and alkylamine additives, the structures of the analytes and temperature on the separations were investigated.
26874335	5	58	theme	Thermodynamic	1015:1027	arg1	parameters					1029:1038	Thermodynamic parameters	1015:1038	Thermodynamic parameters	1015:1038	Thermodynamic parameters were calculated from plots of ln α vs. 1/T.
26874335	2	59	theme	4-chloro-3-methylphenyl	540:562	arg1	tris-					534:538	cellulose tris-	524:538	cellulose tris-(4-chloro-3-methylphenyl carbamate)	524:573	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	59	theme	4-chloro-3-methylphenyl	540:562	arg1	carbamate					564:572	4-chloro-3-methylphenyl carbamate	540:572	4-chloro-3-methylphenyl carbamate	540:572	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	5	60	theme	ln	1070:1071	arg1	α					1073:1073	ln α	1070:1073	ln α	1070:1073	Thermodynamic parameters were calculated from plots of ln α vs. 1/T.
26874335	2	61	theme	derivatives	266:276	arg1	stereoisomers					208:220	The stereoisomers	204:220	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts	204:314	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	62	theme	3,5-dimethylphenyl	406:423	arg1	tris-					400:404	cellulose tris-	390:404	cellulose tris-(3,5-dimethylphenyl carbamate)	390:434	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	62	theme	3,5-dimethylphenyl	406:423	arg1	carbamate					425:433	3,5-dimethylphenyl carbamate	406:433	3,5-dimethylphenyl carbamate	406:433	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	3	63	theme	n-hexane/alcohol/alkylamine	739:765	arg1	phases					774:779	n-hexane/alcohol/alkylamine mobile phases	739:779	n-hexane/alcohol/alkylamine mobile phases in the temperature range 5-40 °C	739:812	The enantioseparations were carried out in normal-phase mode with n-hexane/alcohol/alkylamine mobile phases in the temperature range 5-40 °C.
26874335	7	64	theme	separation	1272:1281	arg1	factor					1283:1288	the separation factor	1268:1288	the separation factor	1268:1288	The enantioseparation was enthalpically controlled, the retention factor and the separation factor decreasing with increasing temperature, but entropically controlled separation was also observed.
26874335	2	65	contain	containing	359:368	arg2	carbamate					477:485	3-chloro-4-methylphenyl carbamate	453:485	3-chloro-4-methylphenyl carbamate	453:485	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	65	contain	containing	359:368	arg2	4-methylbenzoate					505:520	4-methylbenzoate	505:520	4-methylbenzoate	505:520	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	65	contain	containing	359:368	arg2	carbamate					609:617	3,5-dimethylphenyl carbamate	590:617	3,5-dimethylphenyl carbamate	590:617	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	65	contain	containing	359:368	arg1	phases					352:357	chiral stationary phases	334:357	chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate)	334:670	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	65	contain	containing	359:368	arg2	tris-					584:588	amylose tris-	576:588	amylose tris-(3,5-dimethylphenyl carbamate)	576:618	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	65	contain	containing	359:368	arg2	selectors					380:388	chiral selectors	373:388	chiral selectors	373:388	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	65	contain	containing	359:368	arg2	carbamate					564:572	4-chloro-3-methylphenyl carbamate	540:572	4-chloro-3-methylphenyl carbamate	540:572	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	65	contain	containing	359:368	arg2	carbamate					425:433	3,5-dimethylphenyl carbamate	406:433	3,5-dimethylphenyl carbamate	406:433	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	65	contain	containing	359:368	arg2	tris-					400:404	cellulose tris-	390:404	cellulose tris-(3,5-dimethylphenyl carbamate)	390:434	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	65	contain	containing	359:368	arg2	carbamate					661:669	5-chloro-2-methylphenyl carbamate	637:669	5-chloro-2-methylphenyl carbamate	637:669	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	65	contain	containing	359:368	arg2	tris-					631:635	amylose tris-	623:635	amylose tris-(5-chloro-2-methylphenyl carbamate)	623:670	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	65	contain	containing	359:368	arg2	tris-					499:503	cellulose tris-	489:503	cellulose tris-(4-methylbenzoate)	489:521	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	65	contain	containing	359:368	arg2	tris-					447:451	cellulose tris-	437:451	cellulose tris-(3-chloro-4-methylphenyl carbamate)	437:486	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	65	contain	containing	359:368	arg2	tris-					534:538	cellulose tris-	524:538	cellulose tris-(4-chloro-3-methylphenyl carbamate)	524:573	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	2	66	theme	acid	261:264	arg1	derivatives					266:276	five fluorinated cyclic β(3) -amino acid derivatives	225:276	five fluorinated cyclic β(3) -amino acid derivatives	225:276	The stereoisomers of five fluorinated cyclic β(3) -amino acid derivatives and their nonfluorinated counterparts were separated on chiral stationary phases containing as chiral selectors cellulose tris-(3,5-dimethylphenyl carbamate), cellulose tris-(3-chloro-4-methylphenyl carbamate), cellulose tris-(4-methylbenzoate), cellulose tris-(4-chloro-3-methylphenyl carbamate), amylose tris-(3,5-dimethylphenyl carbamate) or amylose tris-(5-chloro-2-methylphenyl carbamate).
26874335	6	67	theme	Δ	1088:1088	arg1	values					1095:1100	The Δ(ΔH°) values	1084:1100	The Δ(ΔH°) values	1084:1100	The Δ(ΔH°) values ranged between -5.0 and +1.6 kJ/mol, while Δ(ΔS°) varied between -12.6 and +5.7 J/mol/K.
25726973	8	0	theme	molecular	1211:1219	arg1	studies					1232:1238	molecular structural studies	1211:1238	molecular structural studies of PRRSV	1211:1247	Our findings help to explain PRRSV infection of cells lacking sialoadhesin and provide a glycan database to facilitate molecular structural studies of PRRSV.
25726973	6	1	theme	oligomers	968:976	arg1	abundance					893:901	an abundance	890:901	an abundance of N-acetylglucosamine (GlcNAc) and N-acetyllactosamine (LacNAc) oligomers in addition to sialic acids	890:1004	Mass spectrometric analysis (HPLC-ESI-MS/MS) of GP5 N-glycans revealed an abundance of N-acetylglucosamine (GlcNAc) and N-acetyllactosamine (LacNAc) oligomers in addition to sialic acids.
25726973	4	2	theme	virion-associated	590:606	arg1	glycans					608:614	the virion-associated glycans	586:614	the virion-associated glycans	586:614	Thus, we purified the PRRSV type 2 prototype strain, VR2332, and analyzed the virion-associated glycans by both biochemical and mass spectrometric methods.
25726973	0	3	theme	glycoprotein	90:101	arg1	profiling					16:24	N-glycosylation profiling	0:24	N-glycosylation profiling of porcine reproductive and respiratory syndrome virus envelope glycoprotein 5	0:103	N-glycosylation profiling of porcine reproductive and respiratory syndrome virus envelope glycoprotein 5.
25726973	4	4	theme	type	540:543	arg1	strain					557:562	the PRRSV type 2 prototype strain	530:562	the PRRSV type 2 prototype strain	530:562	Thus, we purified the PRRSV type 2 prototype strain, VR2332, and analyzed the virion-associated glycans by both biochemical and mass spectrometric methods.
25726973	4	4	theme	type	540:543	arg1	VR2332					565:570	VR2332	565:570	VR2332	565:570	Thus, we purified the PRRSV type 2 prototype strain, VR2332, and analyzed the virion-associated glycans by both biochemical and mass spectrometric methods.
25726973	6	5	theme	GP5	867:869	arg1	N-glycans					871:879	GP5 N-glycans	867:879	GP5 N-glycans	867:879	Mass spectrometric analysis (HPLC-ESI-MS/MS) of GP5 N-glycans revealed an abundance of N-acetylglucosamine (GlcNAc) and N-acetyllactosamine (LacNAc) oligomers in addition to sialic acids.
25726973	4	6	theme	PRRSV	534:538	arg1	strain					557:562	the PRRSV type 2 prototype strain	530:562	the PRRSV type 2 prototype strain	530:562	Thus, we purified the PRRSV type 2 prototype strain, VR2332, and analyzed the virion-associated glycans by both biochemical and mass spectrometric methods.
25726973	4	6	theme	PRRSV	534:538	arg1	VR2332					565:570	VR2332	565:570	VR2332	565:570	Thus, we purified the PRRSV type 2 prototype strain, VR2332, and analyzed the virion-associated glycans by both biochemical and mass spectrometric methods.
25726973	3	7	theme	Structural	402:411	arg1	characterization					413:428	Structural characterization	402:428	Structural characterization of GP5 glycans	402:443	Structural characterization of GP5 glycans would facilitate the mechanistic understanding of these processes.
25726973	5	8	theme	carbohydrate	758:769	arg1	moieties					771:778	the carbohydrate moieties	754:778	the carbohydrate moieties	754:778	Endoglycosidase digestion showed that GP5 was the primary protein substrate, and that the carbohydrate moieties were primarily complex-type N-glycans.
25726973	5	8	theme	carbohydrate	758:769	arg1	N-glycans					808:816	primarily complex-type N-glycans	785:816	primarily complex-type N-glycans	785:816	Endoglycosidase digestion showed that GP5 was the primary protein substrate, and that the carbohydrate moieties were primarily complex-type N-glycans.
25726973	4	9	theme	spectrometric	645:657	arg1	methods					659:665	both biochemical and mass spectrometric methods	619:665	both biochemical and mass spectrometric methods	619:665	Thus, we purified the PRRSV type 2 prototype strain, VR2332, and analyzed the virion-associated glycans by both biochemical and mass spectrometric methods.
25726973	8	10	theme	PRRSV	1243:1247	arg1	studies					1232:1238	molecular structural studies	1211:1238	molecular structural studies of PRRSV	1211:1247	Our findings help to explain PRRSV infection of cells lacking sialoadhesin and provide a glycan database to facilitate molecular structural studies of PRRSV.
25726973	2	11	theme	virus	354:358	arg1	assembly					360:367	virus assembly	354:367	virus assembly	354:367	Glycans of major envelope glycoprotein 5 (GP5) are proposed as important for virus assembly and entry into permissive cells.
25726973	1	12	gly	nonglycosylated	251:265	arg1	proteins					267:274	three nonglycosylated proteins	245:274	three nonglycosylated proteins	245:274	Porcine reproductive and respiratory syndrome virus (PRRSV) is a positive-sense ssRNA virus whose envelope contains four glycoproteins and three nonglycosylated proteins.
25726973	0	13	gly	glycoprotein	90:101	arg1	glycoprotein					90:101	porcine reproductive and respiratory syndrome virus envelope glycoprotein 5	29:103	porcine reproductive and respiratory syndrome virus envelope glycoprotein 5	29:103	N-glycosylation profiling of porcine reproductive and respiratory syndrome virus envelope glycoprotein 5.
25726973	1	14	theme	positive-sense	171:184	arg1	virus					192:196	a positive-sense ssRNA virus	169:196	a positive-sense ssRNA virus whose envelope contains four glycoproteins and three nonglycosylated proteins	169:274	Porcine reproductive and respiratory syndrome virus (PRRSV) is a positive-sense ssRNA virus whose envelope contains four glycoproteins and three nonglycosylated proteins.
25726973	1	14	theme	positive-sense	171:184	arg1	virus					152:156	Porcine reproductive and respiratory syndrome virus	106:156	virus	152:156	Porcine reproductive and respiratory syndrome virus (PRRSV) is a positive-sense ssRNA virus whose envelope contains four glycoproteins and three nonglycosylated proteins.
25726973	1	14	theme	positive-sense	171:184	arg1	reproductive					114:125	reproductive	114:125	reproductive	114:125	Porcine reproductive and respiratory syndrome virus (PRRSV) is a positive-sense ssRNA virus whose envelope contains four glycoproteins and three nonglycosylated proteins.
25726973	2	15	gly	glycoprotein	303:314	arg1	glycoprotein					303:314	major envelope glycoprotein 5	288:316	major envelope glycoprotein 5 (GP5)	288:322	Glycans of major envelope glycoprotein 5 (GP5) are proposed as important for virus assembly and entry into permissive cells.
25726973	2	15	gly	glycoprotein	303:314	arg1	GP5					319:321	GP5	319:321	GP5	319:321	Glycans of major envelope glycoprotein 5 (GP5) are proposed as important for virus assembly and entry into permissive cells.
25726973	2	16	theme	envelope	294:301	arg1	glycoprotein					303:314	major envelope glycoprotein 5	288:316	major envelope glycoprotein 5 (GP5)	288:322	Glycans of major envelope glycoprotein 5 (GP5) are proposed as important for virus assembly and entry into permissive cells.
25726973	2	16	theme	envelope	294:301	arg1	GP5					319:321	GP5	319:321	GP5	319:321	Glycans of major envelope glycoprotein 5 (GP5) are proposed as important for virus assembly and entry into permissive cells.
25726973	1	17	theme	ssRNA	186:190	arg1	virus					192:196	a positive-sense ssRNA virus	169:196	a positive-sense ssRNA virus whose envelope contains four glycoproteins and three nonglycosylated proteins	169:274	Porcine reproductive and respiratory syndrome virus (PRRSV) is a positive-sense ssRNA virus whose envelope contains four glycoproteins and three nonglycosylated proteins.
25726973	1	17	theme	ssRNA	186:190	arg1	virus					152:156	Porcine reproductive and respiratory syndrome virus	106:156	virus	152:156	Porcine reproductive and respiratory syndrome virus (PRRSV) is a positive-sense ssRNA virus whose envelope contains four glycoproteins and three nonglycosylated proteins.
25726973	1	17	theme	ssRNA	186:190	arg1	reproductive					114:125	reproductive	114:125	reproductive	114:125	Porcine reproductive and respiratory syndrome virus (PRRSV) is a positive-sense ssRNA virus whose envelope contains four glycoproteins and three nonglycosylated proteins.
25726973	1	18	gly	glycoproteins	227:239	arg1	glycoproteins					227:239	four glycoproteins	222:239	four glycoproteins	222:239	Porcine reproductive and respiratory syndrome virus (PRRSV) is a positive-sense ssRNA virus whose envelope contains four glycoproteins and three nonglycosylated proteins.
25726973	8	19	theme	PRRSV	1121:1125	arg1	infection					1127:1135	PRRSV infection	1121:1135	PRRSV infection of cells lacking sialoadhesin	1121:1165	Our findings help to explain PRRSV infection of cells lacking sialoadhesin and provide a glycan database to facilitate molecular structural studies of PRRSV.
25726973	6	20	theme	N-acetyllactosamine	939:957	arg1	oligomers					968:976	N-acetylglucosamine (GlcNAc) and N-acetyllactosamine (LacNAc) oligomers	906:976	N-acetylglucosamine (GlcNAc) and N-acetyllactosamine (LacNAc) oligomers	906:976	Mass spectrometric analysis (HPLC-ESI-MS/MS) of GP5 N-glycans revealed an abundance of N-acetylglucosamine (GlcNAc) and N-acetyllactosamine (LacNAc) oligomers in addition to sialic acids.
25726973	0	21	theme	N-glycosylation	0:14	arg1	profiling					16:24	N-glycosylation profiling	0:24	N-glycosylation profiling of porcine reproductive and respiratory syndrome virus envelope glycoprotein 5	0:103	N-glycosylation profiling of porcine reproductive and respiratory syndrome virus envelope glycoprotein 5.
25726973	5	22	theme	Endoglycosidase	668:682	arg1	digestion					684:692	Endoglycosidase digestion	668:692	Endoglycosidase digestion	668:692	Endoglycosidase digestion showed that GP5 was the primary protein substrate, and that the carbohydrate moieties were primarily complex-type N-glycans.
25726973	6	23	theme	spectrometric	824:836	arg1	analysis					838:845	Mass spectrometric analysis	819:845	Mass spectrometric analysis (HPLC-ESI-MS/MS) of GP5 N-glycans	819:879	Mass spectrometric analysis (HPLC-ESI-MS/MS) of GP5 N-glycans revealed an abundance of N-acetylglucosamine (GlcNAc) and N-acetyllactosamine (LacNAc) oligomers in addition to sialic acids.
25726973	6	23	theme	spectrometric	824:836	arg1	HPLC-ESI-MS/MS					848:861	HPLC-ESI-MS/MS	848:861	HPLC-ESI-MS/MS	848:861	Mass spectrometric analysis (HPLC-ESI-MS/MS) of GP5 N-glycans revealed an abundance of N-acetylglucosamine (GlcNAc) and N-acetyllactosamine (LacNAc) oligomers in addition to sialic acids.
25726973	2	24	theme	permissive	384:393	arg1	cells					395:399	permissive cells	384:399	permissive cells	384:399	Glycans of major envelope glycoprotein 5 (GP5) are proposed as important for virus assembly and entry into permissive cells.
25726973	8	25	theme	cells	1140:1144	arg1	infection					1127:1135	PRRSV infection	1121:1135	PRRSV infection of cells lacking sialoadhesin	1121:1165	Our findings help to explain PRRSV infection of cells lacking sialoadhesin and provide a glycan database to facilitate molecular structural studies of PRRSV.
25726973	6	26	theme	Mass	819:822	arg1	analysis					838:845	Mass spectrometric analysis	819:845	Mass spectrometric analysis (HPLC-ESI-MS/MS) of GP5 N-glycans	819:879	Mass spectrometric analysis (HPLC-ESI-MS/MS) of GP5 N-glycans revealed an abundance of N-acetylglucosamine (GlcNAc) and N-acetyllactosamine (LacNAc) oligomers in addition to sialic acids.
25726973	6	26	theme	Mass	819:822	arg1	HPLC-ESI-MS/MS					848:861	HPLC-ESI-MS/MS	848:861	HPLC-ESI-MS/MS	848:861	Mass spectrometric analysis (HPLC-ESI-MS/MS) of GP5 N-glycans revealed an abundance of N-acetylglucosamine (GlcNAc) and N-acetyllactosamine (LacNAc) oligomers in addition to sialic acids.
25726973	2	27	theme	glycoprotein	303:314	arg1	Glycans					277:283	Glycans	277:283	Glycans of major envelope glycoprotein 5 (GP5)	277:322	Glycans of major envelope glycoprotein 5 (GP5) are proposed as important for virus assembly and entry into permissive cells.
25726973	7	28	theme	GlcNAc	1007:1012	arg1	accessibility					1025:1037	GlcNAc and LacNAc accessibility	1007:1037	GlcNAc and LacNAc accessibility to ligands	1007:1048	GlcNAc and LacNAc accessibility to ligands was confirmed by lectin co-precipitation.
25726973	3	29	theme	processes	501:509	arg1	understanding					478:490	the mechanistic understanding	462:490	the mechanistic understanding of these processes	462:509	Structural characterization of GP5 glycans would facilitate the mechanistic understanding of these processes.
25726973	3	30	theme	GP5	433:435	arg1	glycans					437:443	GP5 glycans	433:443	GP5 glycans	433:443	Structural characterization of GP5 glycans would facilitate the mechanistic understanding of these processes.
25726973	0	31	theme	reproductive	37:48	arg1	glycoprotein					90:101	porcine reproductive and respiratory syndrome virus envelope glycoprotein 5	29:103	porcine reproductive and respiratory syndrome virus envelope glycoprotein 5	29:103	N-glycosylation profiling of porcine reproductive and respiratory syndrome virus envelope glycoprotein 5.
25726973	4	32	theme	prototype	547:555	arg1	strain					557:562	the PRRSV type 2 prototype strain	530:562	the PRRSV type 2 prototype strain	530:562	Thus, we purified the PRRSV type 2 prototype strain, VR2332, and analyzed the virion-associated glycans by both biochemical and mass spectrometric methods.
25726973	4	32	theme	prototype	547:555	arg1	VR2332					565:570	VR2332	565:570	VR2332	565:570	Thus, we purified the PRRSV type 2 prototype strain, VR2332, and analyzed the virion-associated glycans by both biochemical and mass spectrometric methods.
25726973	3	33	theme	glycans	437:443	arg1	characterization					413:428	Structural characterization	402:428	Structural characterization of GP5 glycans	402:443	Structural characterization of GP5 glycans would facilitate the mechanistic understanding of these processes.
25726973	6	34	theme	N-acetylglucosamine	906:924	arg1	oligomers					968:976	N-acetylglucosamine (GlcNAc) and N-acetyllactosamine (LacNAc) oligomers	906:976	N-acetylglucosamine (GlcNAc) and N-acetyllactosamine (LacNAc) oligomers	906:976	Mass spectrometric analysis (HPLC-ESI-MS/MS) of GP5 N-glycans revealed an abundance of N-acetylglucosamine (GlcNAc) and N-acetyllactosamine (LacNAc) oligomers in addition to sialic acids.
25726973	4	35	theme	mass	640:643	arg1	methods					659:665	both biochemical and mass spectrometric methods	619:665	both biochemical and mass spectrometric methods	619:665	Thus, we purified the PRRSV type 2 prototype strain, VR2332, and analyzed the virion-associated glycans by both biochemical and mass spectrometric methods.
25726973	7	36	theme	lectin	1067:1072	arg1	co-precipitation					1074:1089	lectin co-precipitation	1067:1089	lectin co-precipitation	1067:1089	GlcNAc and LacNAc accessibility to ligands was confirmed by lectin co-precipitation.
25726973	0	37	theme	syndrome	66:73	arg1	glycoprotein					90:101	porcine reproductive and respiratory syndrome virus envelope glycoprotein 5	29:103	porcine reproductive and respiratory syndrome virus envelope glycoprotein 5	29:103	N-glycosylation profiling of porcine reproductive and respiratory syndrome virus envelope glycoprotein 5.
25726973	6	38	theme	sialic	993:998	arg1	acids					1000:1004	sialic acids	993:1004	sialic acids	993:1004	Mass spectrometric analysis (HPLC-ESI-MS/MS) of GP5 N-glycans revealed an abundance of N-acetylglucosamine (GlcNAc) and N-acetyllactosamine (LacNAc) oligomers in addition to sialic acids.
25726973	5	39	theme	complex-type	795:806	arg1	moieties					771:778	the carbohydrate moieties	754:778	the carbohydrate moieties	754:778	Endoglycosidase digestion showed that GP5 was the primary protein substrate, and that the carbohydrate moieties were primarily complex-type N-glycans.
25726973	5	39	theme	complex-type	795:806	arg1	N-glycans					808:816	primarily complex-type N-glycans	785:816	primarily complex-type N-glycans	785:816	Endoglycosidase digestion showed that GP5 was the primary protein substrate, and that the carbohydrate moieties were primarily complex-type N-glycans.
25726973	8	40	theme	glycan	1181:1186	arg1	database					1188:1195	a glycan database	1179:1195	a glycan database to facilitate molecular structural studies of PRRSV	1179:1247	Our findings help to explain PRRSV infection of cells lacking sialoadhesin and provide a glycan database to facilitate molecular structural studies of PRRSV.
25726973	1	41	theme	respiratory	131:141	arg1	virus					152:156	Porcine reproductive and respiratory syndrome virus	106:156	virus	152:156	Porcine reproductive and respiratory syndrome virus (PRRSV) is a positive-sense ssRNA virus whose envelope contains four glycoproteins and three nonglycosylated proteins.
25726973	1	41	theme	respiratory	131:141	arg1	virus					192:196	a positive-sense ssRNA virus	169:196	a positive-sense ssRNA virus whose envelope contains four glycoproteins and three nonglycosylated proteins	169:274	Porcine reproductive and respiratory syndrome virus (PRRSV) is a positive-sense ssRNA virus whose envelope contains four glycoproteins and three nonglycosylated proteins.
25726973	1	41	theme	respiratory	131:141	arg1	reproductive					114:125	reproductive	114:125	reproductive	114:125	Porcine reproductive and respiratory syndrome virus (PRRSV) is a positive-sense ssRNA virus whose envelope contains four glycoproteins and three nonglycosylated proteins.
25726973	0	42	theme	respiratory	54:64	arg1	glycoprotein					90:101	porcine reproductive and respiratory syndrome virus envelope glycoprotein 5	29:103	porcine reproductive and respiratory syndrome virus envelope glycoprotein 5	29:103	N-glycosylation profiling of porcine reproductive and respiratory syndrome virus envelope glycoprotein 5.
25726973	1	43	theme	syndrome	143:150	arg1	virus					152:156	Porcine reproductive and respiratory syndrome virus	106:156	virus	152:156	Porcine reproductive and respiratory syndrome virus (PRRSV) is a positive-sense ssRNA virus whose envelope contains four glycoproteins and three nonglycosylated proteins.
25726973	1	43	theme	syndrome	143:150	arg1	virus					192:196	a positive-sense ssRNA virus	169:196	a positive-sense ssRNA virus whose envelope contains four glycoproteins and three nonglycosylated proteins	169:274	Porcine reproductive and respiratory syndrome virus (PRRSV) is a positive-sense ssRNA virus whose envelope contains four glycoproteins and three nonglycosylated proteins.
25726973	1	43	theme	syndrome	143:150	arg1	reproductive					114:125	reproductive	114:125	reproductive	114:125	Porcine reproductive and respiratory syndrome virus (PRRSV) is a positive-sense ssRNA virus whose envelope contains four glycoproteins and three nonglycosylated proteins.
25726973	5	44	theme	primary	718:724	arg1	substrate					734:742	the primary protein substrate	714:742	the primary protein substrate	714:742	Endoglycosidase digestion showed that GP5 was the primary protein substrate, and that the carbohydrate moieties were primarily complex-type N-glycans.
25726973	5	44	theme	primary	718:724	arg1	GP5					706:708	GP5	706:708	GP5	706:708	Endoglycosidase digestion showed that GP5 was the primary protein substrate, and that the carbohydrate moieties were primarily complex-type N-glycans.
25726973	2	45	theme	major	288:292	arg1	glycoprotein					303:314	major envelope glycoprotein 5	288:316	major envelope glycoprotein 5 (GP5)	288:322	Glycans of major envelope glycoprotein 5 (GP5) are proposed as important for virus assembly and entry into permissive cells.
25726973	2	45	theme	major	288:292	arg1	GP5					319:321	GP5	319:321	GP5	319:321	Glycans of major envelope glycoprotein 5 (GP5) are proposed as important for virus assembly and entry into permissive cells.
25726973	7	46	theme	LacNAc	1018:1023	arg1	accessibility					1025:1037	GlcNAc and LacNAc accessibility	1007:1037	GlcNAc and LacNAc accessibility to ligands	1007:1048	GlcNAc and LacNAc accessibility to ligands was confirmed by lectin co-precipitation.
25726973	4	47	theme	biochemical	624:634	arg1	methods					659:665	both biochemical and mass spectrometric methods	619:665	both biochemical and mass spectrometric methods	619:665	Thus, we purified the PRRSV type 2 prototype strain, VR2332, and analyzed the virion-associated glycans by both biochemical and mass spectrometric methods.
25726973	5	48	theme	protein	726:732	arg1	substrate					734:742	the primary protein substrate	714:742	the primary protein substrate	714:742	Endoglycosidase digestion showed that GP5 was the primary protein substrate, and that the carbohydrate moieties were primarily complex-type N-glycans.
25726973	5	48	theme	protein	726:732	arg1	GP5					706:708	GP5	706:708	GP5	706:708	Endoglycosidase digestion showed that GP5 was the primary protein substrate, and that the carbohydrate moieties were primarily complex-type N-glycans.
25726973	1	49	contain	contains	213:220	arg2	glycoproteins					227:239	four glycoproteins	222:239	four glycoproteins	222:239	Porcine reproductive and respiratory syndrome virus (PRRSV) is a positive-sense ssRNA virus whose envelope contains four glycoproteins and three nonglycosylated proteins.
25726973	1	49	contain	contains	213:220	arg2	proteins					267:274	three nonglycosylated proteins	245:274	three nonglycosylated proteins	245:274	Porcine reproductive and respiratory syndrome virus (PRRSV) is a positive-sense ssRNA virus whose envelope contains four glycoproteins and three nonglycosylated proteins.
25726973	1	49	contain	contains	213:220	arg1	envelope					204:211	envelope	204:211	envelope	204:211	Porcine reproductive and respiratory syndrome virus (PRRSV) is a positive-sense ssRNA virus whose envelope contains four glycoproteins and three nonglycosylated proteins.
25726973	0	50	theme	envelope	81:88	arg1	glycoprotein					90:101	porcine reproductive and respiratory syndrome virus envelope glycoprotein 5	29:103	porcine reproductive and respiratory syndrome virus envelope glycoprotein 5	29:103	N-glycosylation profiling of porcine reproductive and respiratory syndrome virus envelope glycoprotein 5.
25726973	6	51	theme	N-glycans	871:879	arg1	analysis					838:845	Mass spectrometric analysis	819:845	Mass spectrometric analysis (HPLC-ESI-MS/MS) of GP5 N-glycans	819:879	Mass spectrometric analysis (HPLC-ESI-MS/MS) of GP5 N-glycans revealed an abundance of N-acetylglucosamine (GlcNAc) and N-acetyllactosamine (LacNAc) oligomers in addition to sialic acids.
25726973	6	51	theme	N-glycans	871:879	arg1	HPLC-ESI-MS/MS					848:861	HPLC-ESI-MS/MS	848:861	HPLC-ESI-MS/MS	848:861	Mass spectrometric analysis (HPLC-ESI-MS/MS) of GP5 N-glycans revealed an abundance of N-acetylglucosamine (GlcNAc) and N-acetyllactosamine (LacNAc) oligomers in addition to sialic acids.
25726973	3	52	theme	mechanistic	466:476	arg1	understanding					478:490	the mechanistic understanding	462:490	the mechanistic understanding of these processes	462:509	Structural characterization of GP5 glycans would facilitate the mechanistic understanding of these processes.
25726973	1	53	theme	nonglycosylated	251:265	arg1	proteins					267:274	three nonglycosylated proteins	245:274	three nonglycosylated proteins	245:274	Porcine reproductive and respiratory syndrome virus (PRRSV) is a positive-sense ssRNA virus whose envelope contains four glycoproteins and three nonglycosylated proteins.
25726973	0	54	theme	virus	75:79	arg1	glycoprotein					90:101	porcine reproductive and respiratory syndrome virus envelope glycoprotein 5	29:103	porcine reproductive and respiratory syndrome virus envelope glycoprotein 5	29:103	N-glycosylation profiling of porcine reproductive and respiratory syndrome virus envelope glycoprotein 5.
25726973	8	55	theme	structural	1221:1230	arg1	studies					1232:1238	molecular structural studies	1211:1238	molecular structural studies of PRRSV	1211:1247	Our findings help to explain PRRSV infection of cells lacking sialoadhesin and provide a glycan database to facilitate molecular structural studies of PRRSV.
26732413	2	0	theme	issachenkonii	442:454	arg1	KCTC					456:459	P. issachenkonii KCTC 12958	439:465	P. issachenkonii KCTC 12958	439:465	Here, we report the complete genome of P. issachenkonii KCTC 12958(T) (=KMM 3549(T)=LMG 19697(T)=CIP 106858(T)), which consists of 4,131,541 bp (G+C content of 40.3%) with two chromosomes, 3538 protein-coding genes, 102 tRNAs and 8 rRNA operons.
26732413	3	1	theme	related	660:666	arg1	genes					654:658	Several genes	646:658	Several genes related to glycoside hydrolases, proteases, and bacteriolytic- and hemolytic activities	646:746	Several genes related to glycoside hydrolases, proteases, and bacteriolytic- and hemolytic activities were detected in the genome that help explain how the strain mediates degradation of algal cell wall and decomposes algal polysaccharides into industrially applicable products.
26732413	2	2	theme	P.	439:440	arg1	KCTC					456:459	P. issachenkonii KCTC 12958	439:465	P. issachenkonii KCTC 12958	439:465	Here, we report the complete genome of P. issachenkonii KCTC 12958(T) (=KMM 3549(T)=LMG 19697(T)=CIP 106858(T)), which consists of 4,131,541 bp (G+C content of 40.3%) with two chromosomes, 3538 protein-coding genes, 102 tRNAs and 8 rRNA operons.
26732413	2	3	theme	=CIP	496:499	arg1	T					508:508	T	508:508	T	508:508	Here, we report the complete genome of P. issachenkonii KCTC 12958(T) (=KMM 3549(T)=LMG 19697(T)=CIP 106858(T)), which consists of 4,131,541 bp (G+C content of 40.3%) with two chromosomes, 3538 protein-coding genes, 102 tRNAs and 8 rRNA operons.
26732413	2	3	theme	=CIP	496:499	arg1	106858					501:506	=KMM 3549(T)=LMG 19697(T)=CIP 106858	471:506	=KMM 3549(T)=LMG 19697(T)=CIP 106858(T)	471:509	Here, we report the complete genome of P. issachenkonii KCTC 12958(T) (=KMM 3549(T)=LMG 19697(T)=CIP 106858(T)), which consists of 4,131,541 bp (G+C content of 40.3%) with two chromosomes, 3538 protein-coding genes, 102 tRNAs and 8 rRNA operons.
26732413	3	4	theme	wall	844:847	arg1	degradation					818:828	degradation	818:828	degradation of algal cell wall	818:847	Several genes related to glycoside hydrolases, proteases, and bacteriolytic- and hemolytic activities were detected in the genome that help explain how the strain mediates degradation of algal cell wall and decomposes algal polysaccharides into industrially applicable products.
26732413	2	5	dep	genome	429:434	arg1	T					508:508	T	508:508	T	508:508	Here, we report the complete genome of P. issachenkonii KCTC 12958(T) (=KMM 3549(T)=LMG 19697(T)=CIP 106858(T)), which consists of 4,131,541 bp (G+C content of 40.3%) with two chromosomes, 3538 protein-coding genes, 102 tRNAs and 8 rRNA operons.
26732413	2	5	dep	genome	429:434	arg1	106858					501:506	=KMM 3549(T)=LMG 19697(T)=CIP 106858	471:506	=KMM 3549(T)=LMG 19697(T)=CIP 106858(T)	471:509	Here, we report the complete genome of P. issachenkonii KCTC 12958(T) (=KMM 3549(T)=LMG 19697(T)=CIP 106858(T)), which consists of 4,131,541 bp (G+C content of 40.3%) with two chromosomes, 3538 protein-coding genes, 102 tRNAs and 8 rRNA operons.
26732413	1	6	from	Islands	370:376	arg1	Ocean					393:397	the Pacific Ocean	381:397	the Pacific Ocean	381:397	Pseudoalteromonas issachenkonii is a Gram-negative, rod-shaped, flagellated, aerobic, chemoorganotrophic marine bacterium that was isolated from the thallus of Fucus evanescens (marine brown macroalgae) sampled from the Kraternaya Bight of the Kurile Islands in the Pacific Ocean.
26732413	3	7	theme	Several	646:652	arg1	genes					654:658	Several genes	646:658	Several genes related to glycoside hydrolases, proteases, and bacteriolytic- and hemolytic activities	646:746	Several genes related to glycoside hydrolases, proteases, and bacteriolytic- and hemolytic activities were detected in the genome that help explain how the strain mediates degradation of algal cell wall and decomposes algal polysaccharides into industrially applicable products.
26732413	2	8	theme	T	494:494	arg1	T					508:508	T	508:508	T	508:508	Here, we report the complete genome of P. issachenkonii KCTC 12958(T) (=KMM 3549(T)=LMG 19697(T)=CIP 106858(T)), which consists of 4,131,541 bp (G+C content of 40.3%) with two chromosomes, 3538 protein-coding genes, 102 tRNAs and 8 rRNA operons.
26732413	2	8	theme	T	494:494	arg1	106858					501:506	=KMM 3549(T)=LMG 19697(T)=CIP 106858	471:506	=KMM 3549(T)=LMG 19697(T)=CIP 106858(T)	471:509	Here, we report the complete genome of P. issachenkonii KCTC 12958(T) (=KMM 3549(T)=LMG 19697(T)=CIP 106858(T)), which consists of 4,131,541 bp (G+C content of 40.3%) with two chromosomes, 3538 protein-coding genes, 102 tRNAs and 8 rRNA operons.
26732413	3	9	theme	algal	833:837	arg1	wall					844:847	algal cell wall	833:847	algal cell wall	833:847	Several genes related to glycoside hydrolases, proteases, and bacteriolytic- and hemolytic activities were detected in the genome that help explain how the strain mediates degradation of algal cell wall and decomposes algal polysaccharides into industrially applicable products.
26732413	3	10	theme	glycoside	671:679	arg1	hydrolases					681:690	glycoside hydrolases	671:690	glycoside hydrolases	671:690	Several genes related to glycoside hydrolases, proteases, and bacteriolytic- and hemolytic activities were detected in the genome that help explain how the strain mediates degradation of algal cell wall and decomposes algal polysaccharides into industrially applicable products.
26732413	3	11	theme	cell	839:842	arg1	wall					844:847	algal cell wall	833:847	algal cell wall	833:847	Several genes related to glycoside hydrolases, proteases, and bacteriolytic- and hemolytic activities were detected in the genome that help explain how the strain mediates degradation of algal cell wall and decomposes algal polysaccharides into industrially applicable products.
26732413	2	12	theme	protein-coding	594:607	arg1	genes					609:613	3538 protein-coding genes	589:613	3538 protein-coding genes	589:613	Here, we report the complete genome of P. issachenkonii KCTC 12958(T) (=KMM 3549(T)=LMG 19697(T)=CIP 106858(T)), which consists of 4,131,541 bp (G+C content of 40.3%) with two chromosomes, 3538 protein-coding genes, 102 tRNAs and 8 rRNA operons.
26732413	2	12	theme	protein-coding	594:607	arg1	chromosomes					576:586	two chromosomes	572:586	two chromosomes	572:586	Here, we report the complete genome of P. issachenkonii KCTC 12958(T) (=KMM 3549(T)=LMG 19697(T)=CIP 106858(T)), which consists of 4,131,541 bp (G+C content of 40.3%) with two chromosomes, 3538 protein-coding genes, 102 tRNAs and 8 rRNA operons.
26732413	0	13	theme	=KMM	104:107	arg1	T					114:114	T	114:114	T	114:114	Complete genome of brown algal polysaccharides-degrading Pseudoalteromonas issachenkonii KCTC 12958(T) (=KMM 3549(T)).
26732413	0	13	theme	=KMM	104:107	arg1	3549					109:112	=KMM 3549	104:112	=KMM 3549(T)	104:115	Complete genome of brown algal polysaccharides-degrading Pseudoalteromonas issachenkonii KCTC 12958(T) (=KMM 3549(T)).
26732413	2	14	theme	G+C	545:547	arg1	content					549:555	G+C content	545:555	G+C content of 40.3%	545:564	Here, we report the complete genome of P. issachenkonii KCTC 12958(T) (=KMM 3549(T)=LMG 19697(T)=CIP 106858(T)), which consists of 4,131,541 bp (G+C content of 40.3%) with two chromosomes, 3538 protein-coding genes, 102 tRNAs and 8 rRNA operons.
26732413	3	15	theme	hemolytic	727:735	arg1	activities					737:746	bacteriolytic- and hemolytic activities	708:746	bacteriolytic- and hemolytic activities	708:746	Several genes related to glycoside hydrolases, proteases, and bacteriolytic- and hemolytic activities were detected in the genome that help explain how the strain mediates degradation of algal cell wall and decomposes algal polysaccharides into industrially applicable products.
26732413	1	16	theme	Gram-negative	156:168	arg1	bacterium					231:239	a Gram-negative, rod-shaped, flagellated, aerobic, chemoorganotrophic marine bacterium	154:239	bacterium	231:239	Pseudoalteromonas issachenkonii is a Gram-negative, rod-shaped, flagellated, aerobic, chemoorganotrophic marine bacterium that was isolated from the thallus of Fucus evanescens (marine brown macroalgae) sampled from the Kraternaya Bight of the Kurile Islands in the Pacific Ocean.
26732413	1	16	theme	Gram-negative	156:168	arg1	issachenkonii					137:149	Pseudoalteromonas issachenkonii	119:149	Pseudoalteromonas issachenkonii	119:149	Pseudoalteromonas issachenkonii is a Gram-negative, rod-shaped, flagellated, aerobic, chemoorganotrophic marine bacterium that was isolated from the thallus of Fucus evanescens (marine brown macroalgae) sampled from the Kraternaya Bight of the Kurile Islands in the Pacific Ocean.
26732413	3	17	located	detected	753:760	arg2	genes					654:658	Several genes	646:658	Several genes related to glycoside hydrolases, proteases, and bacteriolytic- and hemolytic activities	646:746	Several genes related to glycoside hydrolases, proteases, and bacteriolytic- and hemolytic activities were detected in the genome that help explain how the strain mediates degradation of algal cell wall and decomposes algal polysaccharides into industrially applicable products.
26732413	3	17	located	detected	753:760	arg1	genome					769:774	the genome	765:774	the genome that help explain how the strain mediates degradation of algal cell wall and decomposes algal polysaccharides into industrially applicable products	765:922	Several genes related to glycoside hydrolases, proteases, and bacteriolytic- and hemolytic activities were detected in the genome that help explain how the strain mediates degradation of algal cell wall and decomposes algal polysaccharides into industrially applicable products.
26732413	0	18	theme	Complete	0:7	arg1	genome					9:14	Complete genome	0:14	Complete genome of brown algal polysaccharides-degrading Pseudoalteromonas issachenkonii KCTC 12958(T) (=KMM 3549(T)).	0:117	Complete genome of brown algal polysaccharides-degrading Pseudoalteromonas issachenkonii KCTC 12958(T) (=KMM 3549(T)).
26732413	1	19	theme	rod-shaped	171:180	arg1	bacterium					231:239	a Gram-negative, rod-shaped, flagellated, aerobic, chemoorganotrophic marine bacterium	154:239	bacterium	231:239	Pseudoalteromonas issachenkonii is a Gram-negative, rod-shaped, flagellated, aerobic, chemoorganotrophic marine bacterium that was isolated from the thallus of Fucus evanescens (marine brown macroalgae) sampled from the Kraternaya Bight of the Kurile Islands in the Pacific Ocean.
26732413	1	19	theme	rod-shaped	171:180	arg1	issachenkonii					137:149	Pseudoalteromonas issachenkonii	119:149	Pseudoalteromonas issachenkonii	119:149	Pseudoalteromonas issachenkonii is a Gram-negative, rod-shaped, flagellated, aerobic, chemoorganotrophic marine bacterium that was isolated from the thallus of Fucus evanescens (marine brown macroalgae) sampled from the Kraternaya Bight of the Kurile Islands in the Pacific Ocean.
26732413	0	20	theme	brown	19:23	arg1	T					100:100	T	100:100	T	100:100	Complete genome of brown algal polysaccharides-degrading Pseudoalteromonas issachenkonii KCTC 12958(T) (=KMM 3549(T)).
26732413	0	20	theme	brown	19:23	arg1	12958					94:98	brown algal polysaccharides-degrading Pseudoalteromonas issachenkonii KCTC 12958	19:98	brown algal polysaccharides-degrading Pseudoalteromonas issachenkonii KCTC 12958(T) (=KMM 3549(T))	19:116	Complete genome of brown algal polysaccharides-degrading Pseudoalteromonas issachenkonii KCTC 12958(T) (=KMM 3549(T)).
26732413	2	21	theme	T	481:481	arg1	T					508:508	T	508:508	T	508:508	Here, we report the complete genome of P. issachenkonii KCTC 12958(T) (=KMM 3549(T)=LMG 19697(T)=CIP 106858(T)), which consists of 4,131,541 bp (G+C content of 40.3%) with two chromosomes, 3538 protein-coding genes, 102 tRNAs and 8 rRNA operons.
26732413	2	21	theme	T	481:481	arg1	106858					501:506	=KMM 3549(T)=LMG 19697(T)=CIP 106858	471:506	=KMM 3549(T)=LMG 19697(T)=CIP 106858(T)	471:509	Here, we report the complete genome of P. issachenkonii KCTC 12958(T) (=KMM 3549(T)=LMG 19697(T)=CIP 106858(T)), which consists of 4,131,541 bp (G+C content of 40.3%) with two chromosomes, 3538 protein-coding genes, 102 tRNAs and 8 rRNA operons.
26732413	3	22	theme	algal	864:868	arg1	polysaccharides					870:884	algal polysaccharides	864:884	algal polysaccharides into industrially applicable products	864:922	Several genes related to glycoside hydrolases, proteases, and bacteriolytic- and hemolytic activities were detected in the genome that help explain how the strain mediates degradation of algal cell wall and decomposes algal polysaccharides into industrially applicable products.
26732413	0	23	dep	12958	94:98	arg1	T					114:114	T	114:114	T	114:114	Complete genome of brown algal polysaccharides-degrading Pseudoalteromonas issachenkonii KCTC 12958(T) (=KMM 3549(T)).
26732413	0	23	dep	12958	94:98	arg1	3549					109:112	=KMM 3549	104:112	=KMM 3549(T)	104:115	Complete genome of brown algal polysaccharides-degrading Pseudoalteromonas issachenkonii KCTC 12958(T) (=KMM 3549(T)).
26732413	2	24	theme	complete	420:427	arg1	T					467:467	T	467:467	T	467:467	Here, we report the complete genome of P. issachenkonii KCTC 12958(T) (=KMM 3549(T)=LMG 19697(T)=CIP 106858(T)), which consists of 4,131,541 bp (G+C content of 40.3%) with two chromosomes, 3538 protein-coding genes, 102 tRNAs and 8 rRNA operons.
26732413	2	24	theme	complete	420:427	arg1	genome					429:434	the complete genome	416:434	the complete genome of P. issachenkonii KCTC 12958(T) (=KMM 3549(T)=LMG 19697(T)=CIP 106858(T))	416:510	Here, we report the complete genome of P. issachenkonii KCTC 12958(T) (=KMM 3549(T)=LMG 19697(T)=CIP 106858(T)), which consists of 4,131,541 bp (G+C content of 40.3%) with two chromosomes, 3538 protein-coding genes, 102 tRNAs and 8 rRNA operons.
26732413	1	25	theme	flagellated	183:193	arg1	bacterium					231:239	a Gram-negative, rod-shaped, flagellated, aerobic, chemoorganotrophic marine bacterium	154:239	bacterium	231:239	Pseudoalteromonas issachenkonii is a Gram-negative, rod-shaped, flagellated, aerobic, chemoorganotrophic marine bacterium that was isolated from the thallus of Fucus evanescens (marine brown macroalgae) sampled from the Kraternaya Bight of the Kurile Islands in the Pacific Ocean.
26732413	1	25	theme	flagellated	183:193	arg1	issachenkonii					137:149	Pseudoalteromonas issachenkonii	119:149	Pseudoalteromonas issachenkonii	119:149	Pseudoalteromonas issachenkonii is a Gram-negative, rod-shaped, flagellated, aerobic, chemoorganotrophic marine bacterium that was isolated from the thallus of Fucus evanescens (marine brown macroalgae) sampled from the Kraternaya Bight of the Kurile Islands in the Pacific Ocean.
26732413	0	26	theme	polysaccharides-degrading	31:55	arg1	T					100:100	T	100:100	T	100:100	Complete genome of brown algal polysaccharides-degrading Pseudoalteromonas issachenkonii KCTC 12958(T) (=KMM 3549(T)).
26732413	0	26	theme	polysaccharides-degrading	31:55	arg1	12958					94:98	brown algal polysaccharides-degrading Pseudoalteromonas issachenkonii KCTC 12958	19:98	brown algal polysaccharides-degrading Pseudoalteromonas issachenkonii KCTC 12958(T) (=KMM 3549(T))	19:116	Complete genome of brown algal polysaccharides-degrading Pseudoalteromonas issachenkonii KCTC 12958(T) (=KMM 3549(T)).
26732413	2	27	theme	3549	476:479	arg1	T					508:508	T	508:508	T	508:508	Here, we report the complete genome of P. issachenkonii KCTC 12958(T) (=KMM 3549(T)=LMG 19697(T)=CIP 106858(T)), which consists of 4,131,541 bp (G+C content of 40.3%) with two chromosomes, 3538 protein-coding genes, 102 tRNAs and 8 rRNA operons.
26732413	2	27	theme	3549	476:479	arg1	106858					501:506	=KMM 3549(T)=LMG 19697(T)=CIP 106858	471:506	=KMM 3549(T)=LMG 19697(T)=CIP 106858(T)	471:509	Here, we report the complete genome of P. issachenkonii KCTC 12958(T) (=KMM 3549(T)=LMG 19697(T)=CIP 106858(T)), which consists of 4,131,541 bp (G+C content of 40.3%) with two chromosomes, 3538 protein-coding genes, 102 tRNAs and 8 rRNA operons.
26732413	1	28	theme	Kurile	363:368	arg1	Islands					370:376	the Kurile Islands	359:376	the Kurile Islands in the Pacific Ocean	359:397	Pseudoalteromonas issachenkonii is a Gram-negative, rod-shaped, flagellated, aerobic, chemoorganotrophic marine bacterium that was isolated from the thallus of Fucus evanescens (marine brown macroalgae) sampled from the Kraternaya Bight of the Kurile Islands in the Pacific Ocean.
26732413	0	29	theme	algal	25:29	arg1	T					100:100	T	100:100	T	100:100	Complete genome of brown algal polysaccharides-degrading Pseudoalteromonas issachenkonii KCTC 12958(T) (=KMM 3549(T)).
26732413	0	29	theme	algal	25:29	arg1	12958					94:98	brown algal polysaccharides-degrading Pseudoalteromonas issachenkonii KCTC 12958	19:98	brown algal polysaccharides-degrading Pseudoalteromonas issachenkonii KCTC 12958(T) (=KMM 3549(T))	19:116	Complete genome of brown algal polysaccharides-degrading Pseudoalteromonas issachenkonii KCTC 12958(T) (=KMM 3549(T)).
26732413	1	30	theme	evanescens	285:294	arg1	thallus					268:274	the thallus	264:274	the thallus of Fucus evanescens (marine brown macroalgae) sampled from the Kraternaya Bight of the Kurile Islands in the Pacific Ocean	264:397	Pseudoalteromonas issachenkonii is a Gram-negative, rod-shaped, flagellated, aerobic, chemoorganotrophic marine bacterium that was isolated from the thallus of Fucus evanescens (marine brown macroalgae) sampled from the Kraternaya Bight of the Kurile Islands in the Pacific Ocean.
26732413	2	31	theme	=KMM	471:474	arg1	T					508:508	T	508:508	T	508:508	Here, we report the complete genome of P. issachenkonii KCTC 12958(T) (=KMM 3549(T)=LMG 19697(T)=CIP 106858(T)), which consists of 4,131,541 bp (G+C content of 40.3%) with two chromosomes, 3538 protein-coding genes, 102 tRNAs and 8 rRNA operons.
26732413	2	31	theme	=KMM	471:474	arg1	106858					501:506	=KMM 3549(T)=LMG 19697(T)=CIP 106858	471:506	=KMM 3549(T)=LMG 19697(T)=CIP 106858(T)	471:509	Here, we report the complete genome of P. issachenkonii KCTC 12958(T) (=KMM 3549(T)=LMG 19697(T)=CIP 106858(T)), which consists of 4,131,541 bp (G+C content of 40.3%) with two chromosomes, 3538 protein-coding genes, 102 tRNAs and 8 rRNA operons.
26732413	2	32	theme	%	564:564	arg1	content					549:555	G+C content	545:555	G+C content of 40.3%	545:564	Here, we report the complete genome of P. issachenkonii KCTC 12958(T) (=KMM 3549(T)=LMG 19697(T)=CIP 106858(T)), which consists of 4,131,541 bp (G+C content of 40.3%) with two chromosomes, 3538 protein-coding genes, 102 tRNAs and 8 rRNA operons.
26732413	1	33	theme	aerobic	196:202	arg1	bacterium					231:239	a Gram-negative, rod-shaped, flagellated, aerobic, chemoorganotrophic marine bacterium	154:239	bacterium	231:239	Pseudoalteromonas issachenkonii is a Gram-negative, rod-shaped, flagellated, aerobic, chemoorganotrophic marine bacterium that was isolated from the thallus of Fucus evanescens (marine brown macroalgae) sampled from the Kraternaya Bight of the Kurile Islands in the Pacific Ocean.
26732413	1	33	theme	aerobic	196:202	arg1	issachenkonii					137:149	Pseudoalteromonas issachenkonii	119:149	Pseudoalteromonas issachenkonii	119:149	Pseudoalteromonas issachenkonii is a Gram-negative, rod-shaped, flagellated, aerobic, chemoorganotrophic marine bacterium that was isolated from the thallus of Fucus evanescens (marine brown macroalgae) sampled from the Kraternaya Bight of the Kurile Islands in the Pacific Ocean.
26732413	0	34	theme	issachenkonii	75:87	arg1	T					100:100	T	100:100	T	100:100	Complete genome of brown algal polysaccharides-degrading Pseudoalteromonas issachenkonii KCTC 12958(T) (=KMM 3549(T)).
26732413	0	34	theme	issachenkonii	75:87	arg1	12958					94:98	brown algal polysaccharides-degrading Pseudoalteromonas issachenkonii KCTC 12958	19:98	brown algal polysaccharides-degrading Pseudoalteromonas issachenkonii KCTC 12958(T) (=KMM 3549(T))	19:116	Complete genome of brown algal polysaccharides-degrading Pseudoalteromonas issachenkonii KCTC 12958(T) (=KMM 3549(T)).
26732413	2	35	with	bp	541:542	arg1	operons					637:643	8 rRNA operons	630:643	8 rRNA operons	630:643	Here, we report the complete genome of P. issachenkonii KCTC 12958(T) (=KMM 3549(T)=LMG 19697(T)=CIP 106858(T)), which consists of 4,131,541 bp (G+C content of 40.3%) with two chromosomes, 3538 protein-coding genes, 102 tRNAs and 8 rRNA operons.
26732413	2	35	with	bp	541:542	arg1	genes					609:613	3538 protein-coding genes	589:613	3538 protein-coding genes	589:613	Here, we report the complete genome of P. issachenkonii KCTC 12958(T) (=KMM 3549(T)=LMG 19697(T)=CIP 106858(T)), which consists of 4,131,541 bp (G+C content of 40.3%) with two chromosomes, 3538 protein-coding genes, 102 tRNAs and 8 rRNA operons.
26732413	2	35	with	bp	541:542	arg1	tRNAs					620:624	102 tRNAs	616:624	102 tRNAs	616:624	Here, we report the complete genome of P. issachenkonii KCTC 12958(T) (=KMM 3549(T)=LMG 19697(T)=CIP 106858(T)), which consists of 4,131,541 bp (G+C content of 40.3%) with two chromosomes, 3538 protein-coding genes, 102 tRNAs and 8 rRNA operons.
26732413	2	35	with	bp	541:542	arg1	chromosomes					576:586	two chromosomes	572:586	two chromosomes	572:586	Here, we report the complete genome of P. issachenkonii KCTC 12958(T) (=KMM 3549(T)=LMG 19697(T)=CIP 106858(T)), which consists of 4,131,541 bp (G+C content of 40.3%) with two chromosomes, 3538 protein-coding genes, 102 tRNAs and 8 rRNA operons.
26732413	2	36	theme	KCTC	456:459	arg1	T					467:467	T	467:467	T	467:467	Here, we report the complete genome of P. issachenkonii KCTC 12958(T) (=KMM 3549(T)=LMG 19697(T)=CIP 106858(T)), which consists of 4,131,541 bp (G+C content of 40.3%) with two chromosomes, 3538 protein-coding genes, 102 tRNAs and 8 rRNA operons.
26732413	2	36	theme	KCTC	456:459	arg1	genome					429:434	the complete genome	416:434	the complete genome of P. issachenkonii KCTC 12958(T) (=KMM 3549(T)=LMG 19697(T)=CIP 106858(T))	416:510	Here, we report the complete genome of P. issachenkonii KCTC 12958(T) (=KMM 3549(T)=LMG 19697(T)=CIP 106858(T)), which consists of 4,131,541 bp (G+C content of 40.3%) with two chromosomes, 3538 protein-coding genes, 102 tRNAs and 8 rRNA operons.
26732413	0	37	theme	Pseudoalteromonas	57:73	arg1	T					100:100	T	100:100	T	100:100	Complete genome of brown algal polysaccharides-degrading Pseudoalteromonas issachenkonii KCTC 12958(T) (=KMM 3549(T)).
26732413	0	37	theme	Pseudoalteromonas	57:73	arg1	12958					94:98	brown algal polysaccharides-degrading Pseudoalteromonas issachenkonii KCTC 12958	19:98	brown algal polysaccharides-degrading Pseudoalteromonas issachenkonii KCTC 12958(T) (=KMM 3549(T))	19:116	Complete genome of brown algal polysaccharides-degrading Pseudoalteromonas issachenkonii KCTC 12958(T) (=KMM 3549(T)).
26732413	1	38	theme	marine	297:302	arg1	macroalgae					310:319	marine brown macroalgae	297:319	marine brown macroalgae	297:319	Pseudoalteromonas issachenkonii is a Gram-negative, rod-shaped, flagellated, aerobic, chemoorganotrophic marine bacterium that was isolated from the thallus of Fucus evanescens (marine brown macroalgae) sampled from the Kraternaya Bight of the Kurile Islands in the Pacific Ocean.
26732413	1	38	theme	marine	297:302	arg1	evanescens					285:294	Fucus evanescens	279:294	Fucus evanescens (marine brown macroalgae) sampled from the Kraternaya Bight of the Kurile Islands in the Pacific Ocean	279:397	Pseudoalteromonas issachenkonii is a Gram-negative, rod-shaped, flagellated, aerobic, chemoorganotrophic marine bacterium that was isolated from the thallus of Fucus evanescens (marine brown macroalgae) sampled from the Kraternaya Bight of the Kurile Islands in the Pacific Ocean.
26732413	2	39	theme	19697	488:492	arg1	T					508:508	T	508:508	T	508:508	Here, we report the complete genome of P. issachenkonii KCTC 12958(T) (=KMM 3549(T)=LMG 19697(T)=CIP 106858(T)), which consists of 4,131,541 bp (G+C content of 40.3%) with two chromosomes, 3538 protein-coding genes, 102 tRNAs and 8 rRNA operons.
26732413	2	39	theme	19697	488:492	arg1	106858					501:506	=KMM 3549(T)=LMG 19697(T)=CIP 106858	471:506	=KMM 3549(T)=LMG 19697(T)=CIP 106858(T)	471:509	Here, we report the complete genome of P. issachenkonii KCTC 12958(T) (=KMM 3549(T)=LMG 19697(T)=CIP 106858(T)), which consists of 4,131,541 bp (G+C content of 40.3%) with two chromosomes, 3538 protein-coding genes, 102 tRNAs and 8 rRNA operons.
26732413	2	40	dep	bp	541:542	arg1	content					549:555	G+C content	545:555	G+C content of 40.3%	545:564	Here, we report the complete genome of P. issachenkonii KCTC 12958(T) (=KMM 3549(T)=LMG 19697(T)=CIP 106858(T)), which consists of 4,131,541 bp (G+C content of 40.3%) with two chromosomes, 3538 protein-coding genes, 102 tRNAs and 8 rRNA operons.
26732413	1	41	theme	brown	304:308	arg1	macroalgae					310:319	marine brown macroalgae	297:319	marine brown macroalgae	297:319	Pseudoalteromonas issachenkonii is a Gram-negative, rod-shaped, flagellated, aerobic, chemoorganotrophic marine bacterium that was isolated from the thallus of Fucus evanescens (marine brown macroalgae) sampled from the Kraternaya Bight of the Kurile Islands in the Pacific Ocean.
26732413	1	41	theme	brown	304:308	arg1	evanescens					285:294	Fucus evanescens	279:294	Fucus evanescens (marine brown macroalgae) sampled from the Kraternaya Bight of the Kurile Islands in the Pacific Ocean	279:397	Pseudoalteromonas issachenkonii is a Gram-negative, rod-shaped, flagellated, aerobic, chemoorganotrophic marine bacterium that was isolated from the thallus of Fucus evanescens (marine brown macroalgae) sampled from the Kraternaya Bight of the Kurile Islands in the Pacific Ocean.
26732413	2	42	theme	=LMG	483:486	arg1	T					508:508	T	508:508	T	508:508	Here, we report the complete genome of P. issachenkonii KCTC 12958(T) (=KMM 3549(T)=LMG 19697(T)=CIP 106858(T)), which consists of 4,131,541 bp (G+C content of 40.3%) with two chromosomes, 3538 protein-coding genes, 102 tRNAs and 8 rRNA operons.
26732413	2	42	theme	=LMG	483:486	arg1	106858					501:506	=KMM 3549(T)=LMG 19697(T)=CIP 106858	471:506	=KMM 3549(T)=LMG 19697(T)=CIP 106858(T)	471:509	Here, we report the complete genome of P. issachenkonii KCTC 12958(T) (=KMM 3549(T)=LMG 19697(T)=CIP 106858(T)), which consists of 4,131,541 bp (G+C content of 40.3%) with two chromosomes, 3538 protein-coding genes, 102 tRNAs and 8 rRNA operons.
26732413	1	43	theme	Pacific	385:391	arg1	Ocean					393:397	the Pacific Ocean	381:397	the Pacific Ocean	381:397	Pseudoalteromonas issachenkonii is a Gram-negative, rod-shaped, flagellated, aerobic, chemoorganotrophic marine bacterium that was isolated from the thallus of Fucus evanescens (marine brown macroalgae) sampled from the Kraternaya Bight of the Kurile Islands in the Pacific Ocean.
26732413	3	44	theme	applicable	904:913	arg1	products					915:922	industrially applicable products	891:922	industrially applicable products	891:922	Several genes related to glycoside hydrolases, proteases, and bacteriolytic- and hemolytic activities were detected in the genome that help explain how the strain mediates degradation of algal cell wall and decomposes algal polysaccharides into industrially applicable products.
26732413	2	45	theme	rRNA	632:635	arg1	operons					637:643	8 rRNA operons	630:643	8 rRNA operons	630:643	Here, we report the complete genome of P. issachenkonii KCTC 12958(T) (=KMM 3549(T)=LMG 19697(T)=CIP 106858(T)), which consists of 4,131,541 bp (G+C content of 40.3%) with two chromosomes, 3538 protein-coding genes, 102 tRNAs and 8 rRNA operons.
26732413	2	45	theme	rRNA	632:635	arg1	chromosomes					576:586	two chromosomes	572:586	two chromosomes	572:586	Here, we report the complete genome of P. issachenkonii KCTC 12958(T) (=KMM 3549(T)=LMG 19697(T)=CIP 106858(T)), which consists of 4,131,541 bp (G+C content of 40.3%) with two chromosomes, 3538 protein-coding genes, 102 tRNAs and 8 rRNA operons.
26732413	1	46	theme	chemoorganotrophic	205:222	arg1	bacterium					231:239	a Gram-negative, rod-shaped, flagellated, aerobic, chemoorganotrophic marine bacterium	154:239	bacterium	231:239	Pseudoalteromonas issachenkonii is a Gram-negative, rod-shaped, flagellated, aerobic, chemoorganotrophic marine bacterium that was isolated from the thallus of Fucus evanescens (marine brown macroalgae) sampled from the Kraternaya Bight of the Kurile Islands in the Pacific Ocean.
26732413	1	46	theme	chemoorganotrophic	205:222	arg1	issachenkonii					137:149	Pseudoalteromonas issachenkonii	119:149	Pseudoalteromonas issachenkonii	119:149	Pseudoalteromonas issachenkonii is a Gram-negative, rod-shaped, flagellated, aerobic, chemoorganotrophic marine bacterium that was isolated from the thallus of Fucus evanescens (marine brown macroalgae) sampled from the Kraternaya Bight of the Kurile Islands in the Pacific Ocean.
26732413	0	47	theme	12958	94:98	arg1	genome					9:14	Complete genome	0:14	Complete genome of brown algal polysaccharides-degrading Pseudoalteromonas issachenkonii KCTC 12958(T) (=KMM 3549(T)).	0:117	Complete genome of brown algal polysaccharides-degrading Pseudoalteromonas issachenkonii KCTC 12958(T) (=KMM 3549(T)).
26732413	1	48	theme	marine	224:229	arg1	bacterium					231:239	a Gram-negative, rod-shaped, flagellated, aerobic, chemoorganotrophic marine bacterium	154:239	bacterium	231:239	Pseudoalteromonas issachenkonii is a Gram-negative, rod-shaped, flagellated, aerobic, chemoorganotrophic marine bacterium that was isolated from the thallus of Fucus evanescens (marine brown macroalgae) sampled from the Kraternaya Bight of the Kurile Islands in the Pacific Ocean.
26732413	1	48	theme	marine	224:229	arg1	issachenkonii					137:149	Pseudoalteromonas issachenkonii	119:149	Pseudoalteromonas issachenkonii	119:149	Pseudoalteromonas issachenkonii is a Gram-negative, rod-shaped, flagellated, aerobic, chemoorganotrophic marine bacterium that was isolated from the thallus of Fucus evanescens (marine brown macroalgae) sampled from the Kraternaya Bight of the Kurile Islands in the Pacific Ocean.
26732413	0	49	theme	KCTC	89:92	arg1	T					100:100	T	100:100	T	100:100	Complete genome of brown algal polysaccharides-degrading Pseudoalteromonas issachenkonii KCTC 12958(T) (=KMM 3549(T)).
26732413	0	49	theme	KCTC	89:92	arg1	12958					94:98	brown algal polysaccharides-degrading Pseudoalteromonas issachenkonii KCTC 12958	19:98	brown algal polysaccharides-degrading Pseudoalteromonas issachenkonii KCTC 12958(T) (=KMM 3549(T))	19:116	Complete genome of brown algal polysaccharides-degrading Pseudoalteromonas issachenkonii KCTC 12958(T) (=KMM 3549(T)).
26732413	1	50	attach	isolated	250:257	arg2	issachenkonii					137:149	Pseudoalteromonas issachenkonii	119:149	Pseudoalteromonas issachenkonii	119:149	Pseudoalteromonas issachenkonii is a Gram-negative, rod-shaped, flagellated, aerobic, chemoorganotrophic marine bacterium that was isolated from the thallus of Fucus evanescens (marine brown macroalgae) sampled from the Kraternaya Bight of the Kurile Islands in the Pacific Ocean.
26732413	1	50	attach	isolated	250:257	arg2	bacterium					231:239	a Gram-negative, rod-shaped, flagellated, aerobic, chemoorganotrophic marine bacterium	154:239	bacterium	231:239	Pseudoalteromonas issachenkonii is a Gram-negative, rod-shaped, flagellated, aerobic, chemoorganotrophic marine bacterium that was isolated from the thallus of Fucus evanescens (marine brown macroalgae) sampled from the Kraternaya Bight of the Kurile Islands in the Pacific Ocean.
26732413	1	50	attach	isolated	250:257	arg1	thallus					268:274	the thallus	264:274	the thallus of Fucus evanescens (marine brown macroalgae) sampled from the Kraternaya Bight of the Kurile Islands in the Pacific Ocean	264:397	Pseudoalteromonas issachenkonii is a Gram-negative, rod-shaped, flagellated, aerobic, chemoorganotrophic marine bacterium that was isolated from the thallus of Fucus evanescens (marine brown macroalgae) sampled from the Kraternaya Bight of the Kurile Islands in the Pacific Ocean.
26732413	1	51	theme	Pseudoalteromonas	119:135	arg1	bacterium					231:239	a Gram-negative, rod-shaped, flagellated, aerobic, chemoorganotrophic marine bacterium	154:239	bacterium	231:239	Pseudoalteromonas issachenkonii is a Gram-negative, rod-shaped, flagellated, aerobic, chemoorganotrophic marine bacterium that was isolated from the thallus of Fucus evanescens (marine brown macroalgae) sampled from the Kraternaya Bight of the Kurile Islands in the Pacific Ocean.
26732413	1	51	theme	Pseudoalteromonas	119:135	arg1	issachenkonii					137:149	Pseudoalteromonas issachenkonii	119:149	Pseudoalteromonas issachenkonii	119:149	Pseudoalteromonas issachenkonii is a Gram-negative, rod-shaped, flagellated, aerobic, chemoorganotrophic marine bacterium that was isolated from the thallus of Fucus evanescens (marine brown macroalgae) sampled from the Kraternaya Bight of the Kurile Islands in the Pacific Ocean.
26732413	3	52	theme	bacteriolytic-	708:721	arg1	activities					737:746	bacteriolytic- and hemolytic activities	708:746	bacteriolytic- and hemolytic activities	708:746	Several genes related to glycoside hydrolases, proteases, and bacteriolytic- and hemolytic activities were detected in the genome that help explain how the strain mediates degradation of algal cell wall and decomposes algal polysaccharides into industrially applicable products.
28528944	0	0	theme	officinale	70:79	arg1	stem					51:54	the stem	47:54	the stem of Dendrobium officinale and anti-angiogenesis activities of its sulfated derivative	47:139	Structure analysis of a novel heteroxylan from the stem of Dendrobium officinale and anti-angiogenesis activities of its sulfated derivative.
28528944	4	1	from	glucose	612:618	arg1	ratio					713:717	a ratio	711:717	a ratio of 8.9:62.7:8.5:12.3:3.9:3.7	711:746	It composed of arabinose, xylose, glucose and 4-O-methylglucuronic acid (4-MGA) as well as trace amount of rhamnose and galactose in a ratio of 8.9:62.7:8.5:12.3:3.9:3.7.
28528944	5	2	link	1,4-linked	838:847	arg1	α-d-glucose					849:859	1,4-linked α-d-glucose	838:859	1,4-linked α-d-glucose	838:859	We further showed that it contained a backbone of 1,4-linked β-d-xylan, with branches of 1,4-linked α-d-glucose, 1,3-linked α-l-rhamnose, and terminal-linked α-l-arabinose, β-d-galactose, 4-MGA, and β-d-xylose directly or indirectly attached to C-2 position of glycosyl residues on backbone.
28528944	7	3	from	concentration	1362:1374	arg1	way					1405:1407	a dose-dependent way	1388:1407	a dose-dependent way without significant cytotoxity	1388:1438	Bioactivity tests suggested that the sulfated polysaccharide could significantly disrupt tube formation and inhibit the migration of human microvascular endothelial cells (HMEC-1) at a low concentration (0.29μM) in a dose-dependent way without significant cytotoxity.
28528944	2	4	theme	major	360:364	arg1	substances					376:385	the major bioactive substances	356:385	the major bioactive substances from this herb	356:400	Although evidences showed polysaccharide is one of the major bioactive substances from this herb, several of them were homogenous with fine structures elucidated.
28528944	0	5	theme	Dendrobium	59:68	arg1	officinale					70:79	Dendrobium officinale and anti-angiogenesis activities	59:112	officinale	70:79	Structure analysis of a novel heteroxylan from the stem of Dendrobium officinale and anti-angiogenesis activities of its sulfated derivative.
28528944	0	6	from	analysis	10:17	arg1	stem					51:54	the stem	47:54	the stem of Dendrobium officinale and anti-angiogenesis activities of its sulfated derivative	47:139	Structure analysis of a novel heteroxylan from the stem of Dendrobium officinale and anti-angiogenesis activities of its sulfated derivative.
28528944	5	7	theme	α-l-arabinose	907:919	arg1	branches					826:833	branches	826:833	branches of 1,4-linked α-d-glucose, 1,3-linked α-l-rhamnose, and terminal-linked α-l-arabinose, β-d-galactose, 4-MGA, and β-d-xylose directly or indirectly attached to C-2 position of glycosyl residues on backbone	826:1038	We further showed that it contained a backbone of 1,4-linked β-d-xylan, with branches of 1,4-linked α-d-glucose, 1,3-linked α-l-rhamnose, and terminal-linked α-l-arabinose, β-d-galactose, 4-MGA, and β-d-xylose directly or indirectly attached to C-2 position of glycosyl residues on backbone.
28528944	3	8	theme	novel	495:499	arg1	heteroxylan					513:523	a novel homogeneous heteroxylan	493:523	a novel homogeneous heteroxylan obtained from alkali-extracted crude polysaccharide	493:575	In this study, we showed a novel homogeneous heteroxylan obtained from alkali-extracted crude polysaccharide.
28528944	4	9	theme	8.9:62.7:8.5:12.3:3.9:3.7	722:746	arg1	ratio					713:717	a ratio	711:717	a ratio of 8.9:62.7:8.5:12.3:3.9:3.7	711:746	It composed of arabinose, xylose, glucose and 4-O-methylglucuronic acid (4-MGA) as well as trace amount of rhamnose and galactose in a ratio of 8.9:62.7:8.5:12.3:3.9:3.7.
28528944	0	10	theme	anti-angiogenesis	85:101	arg1	activities					103:112	Dendrobium officinale and anti-angiogenesis activities	59:112	activities	103:112	Structure analysis of a novel heteroxylan from the stem of Dendrobium officinale and anti-angiogenesis activities of its sulfated derivative.
28528944	3	11	theme	homogeneous	501:511	arg1	heteroxylan					513:523	a novel homogeneous heteroxylan	493:523	a novel homogeneous heteroxylan obtained from alkali-extracted crude polysaccharide	493:575	In this study, we showed a novel homogeneous heteroxylan obtained from alkali-extracted crude polysaccharide.
28528944	3	12	theme	alkali-extracted	539:554	arg1	polysaccharide					562:575	alkali-extracted crude polysaccharide	539:575	alkali-extracted crude polysaccharide	539:575	In this study, we showed a novel homogeneous heteroxylan obtained from alkali-extracted crude polysaccharide.
28528944	4	13	from	amount	675:680	arg1	ratio					713:717	a ratio	711:717	a ratio of 8.9:62.7:8.5:12.3:3.9:3.7	711:746	It composed of arabinose, xylose, glucose and 4-O-methylglucuronic acid (4-MGA) as well as trace amount of rhamnose and galactose in a ratio of 8.9:62.7:8.5:12.3:3.9:3.7.
28528944	2	14	theme	showed	324:329	arg1	polysaccharide					331:344	evidences showed polysaccharide	314:344	evidences showed polysaccharide	314:344	Although evidences showed polysaccharide is one of the major bioactive substances from this herb, several of them were homogenous with fine structures elucidated.
28528944	5	15	theme	β-d-xylan	810:818	arg1	backbone					787:794	a backbone	785:794	a backbone of 1,4-linked β-d-xylan	785:818	We further showed that it contained a backbone of 1,4-linked β-d-xylan, with branches of 1,4-linked α-d-glucose, 1,3-linked α-l-rhamnose, and terminal-linked α-l-arabinose, β-d-galactose, 4-MGA, and β-d-xylose directly or indirectly attached to C-2 position of glycosyl residues on backbone.
28528944	3	16	theme	crude	556:560	arg1	polysaccharide					562:575	alkali-extracted crude polysaccharide	539:575	alkali-extracted crude polysaccharide	539:575	In this study, we showed a novel homogeneous heteroxylan obtained from alkali-extracted crude polysaccharide.
28528944	6	17	theme	chlorosulfonic	1130:1143	arg1	acid					1145:1148	chlorosulfonic acid	1130:1148	the chlorosulfonic acid (CSA)-pyridine method	1126:1170	The sulfated derivative with substitution degree about 0.9 was prepared according to the chlorosulfonic acid (CSA)-pyridine method.
28528944	6	17	theme	chlorosulfonic	1130:1143	arg1	CSA					1151:1153	CSA	1151:1153	CSA	1151:1153	The sulfated derivative with substitution degree about 0.9 was prepared according to the chlorosulfonic acid (CSA)-pyridine method.
28528944	4	18	theme	rhamnose	685:692	arg1	acid					645:648	4-O-methylglucuronic acid	624:648	4-O-methylglucuronic acid (4-MGA)	624:656	It composed of arabinose, xylose, glucose and 4-O-methylglucuronic acid (4-MGA) as well as trace amount of rhamnose and galactose in a ratio of 8.9:62.7:8.5:12.3:3.9:3.7.
28528944	4	18	theme	rhamnose	685:692	arg1	xylose					604:609	xylose	604:609	xylose	604:609	It composed of arabinose, xylose, glucose and 4-O-methylglucuronic acid (4-MGA) as well as trace amount of rhamnose and galactose in a ratio of 8.9:62.7:8.5:12.3:3.9:3.7.
28528944	4	18	theme	rhamnose	685:692	arg1	4-MGA					651:655	4-MGA	651:655	4-MGA	651:655	It composed of arabinose, xylose, glucose and 4-O-methylglucuronic acid (4-MGA) as well as trace amount of rhamnose and galactose in a ratio of 8.9:62.7:8.5:12.3:3.9:3.7.
28528944	4	18	theme	rhamnose	685:692	arg1	amount					675:680	trace amount	669:680	trace amount of rhamnose and galactose in a ratio of 8.9:62.7:8.5:12.3:3.9:3.7	669:746	It composed of arabinose, xylose, glucose and 4-O-methylglucuronic acid (4-MGA) as well as trace amount of rhamnose and galactose in a ratio of 8.9:62.7:8.5:12.3:3.9:3.7.
28528944	4	18	theme	rhamnose	685:692	arg1	rhamnose					685:692	rhamnose	685:692	rhamnose	685:692	It composed of arabinose, xylose, glucose and 4-O-methylglucuronic acid (4-MGA) as well as trace amount of rhamnose and galactose in a ratio of 8.9:62.7:8.5:12.3:3.9:3.7.
28528944	4	18	theme	rhamnose	685:692	arg1	glucose					612:618	glucose	612:618	glucose	612:618	It composed of arabinose, xylose, glucose and 4-O-methylglucuronic acid (4-MGA) as well as trace amount of rhamnose and galactose in a ratio of 8.9:62.7:8.5:12.3:3.9:3.7.
28528944	4	18	theme	rhamnose	685:692	arg1	arabinose					593:601	arabinose	593:601	arabinose	593:601	It composed of arabinose, xylose, glucose and 4-O-methylglucuronic acid (4-MGA) as well as trace amount of rhamnose and galactose in a ratio of 8.9:62.7:8.5:12.3:3.9:3.7.
28528944	4	18	theme	rhamnose	685:692	arg1	galactose					698:706	galactose	698:706	galactose	698:706	It composed of arabinose, xylose, glucose and 4-O-methylglucuronic acid (4-MGA) as well as trace amount of rhamnose and galactose in a ratio of 8.9:62.7:8.5:12.3:3.9:3.7.
28528944	7	19	theme	microvascular	1312:1324	arg1	HMEC-1					1345:1350	HMEC-1	1345:1350	HMEC-1	1345:1350	Bioactivity tests suggested that the sulfated polysaccharide could significantly disrupt tube formation and inhibit the migration of human microvascular endothelial cells (HMEC-1) at a low concentration (0.29μM) in a dose-dependent way without significant cytotoxity.
28528944	7	19	theme	microvascular	1312:1324	arg1	cells					1338:1342	human microvascular endothelial cells	1306:1342	human microvascular endothelial cells (HMEC-1)	1306:1351	Bioactivity tests suggested that the sulfated polysaccharide could significantly disrupt tube formation and inhibit the migration of human microvascular endothelial cells (HMEC-1) at a low concentration (0.29μM) in a dose-dependent way without significant cytotoxity.
28528944	2	20	theme	them	414:417	arg1	several					403:409	several	403:409	several	403:409	Although evidences showed polysaccharide is one of the major bioactive substances from this herb, several of them were homogenous with fine structures elucidated.
28528944	5	21	theme	α-l-rhamnose	873:884	arg1	branches					826:833	branches	826:833	branches of 1,4-linked α-d-glucose, 1,3-linked α-l-rhamnose, and terminal-linked α-l-arabinose, β-d-galactose, 4-MGA, and β-d-xylose directly or indirectly attached to C-2 position of glycosyl residues on backbone	826:1038	We further showed that it contained a backbone of 1,4-linked β-d-xylan, with branches of 1,4-linked α-d-glucose, 1,3-linked α-l-rhamnose, and terminal-linked α-l-arabinose, β-d-galactose, 4-MGA, and β-d-xylose directly or indirectly attached to C-2 position of glycosyl residues on backbone.
28528944	7	22	theme	low	1358:1360	arg1	0.29μM					1377:1382	0.29μM	1377:1382	0.29μM	1377:1382	Bioactivity tests suggested that the sulfated polysaccharide could significantly disrupt tube formation and inhibit the migration of human microvascular endothelial cells (HMEC-1) at a low concentration (0.29μM) in a dose-dependent way without significant cytotoxity.
28528944	7	22	theme	low	1358:1360	arg1	concentration					1362:1374	a low concentration	1356:1374	a low concentration (0.29μM) in a dose-dependent way without significant cytotoxity	1356:1438	Bioactivity tests suggested that the sulfated polysaccharide could significantly disrupt tube formation and inhibit the migration of human microvascular endothelial cells (HMEC-1) at a low concentration (0.29μM) in a dose-dependent way without significant cytotoxity.
28528944	0	23	theme	activities	103:112	arg1	stem					51:54	the stem	47:54	the stem of Dendrobium officinale and anti-angiogenesis activities of its sulfated derivative	47:139	Structure analysis of a novel heteroxylan from the stem of Dendrobium officinale and anti-angiogenesis activities of its sulfated derivative.
28528944	0	24	theme	Structure	0:8	arg1	analysis					10:17	Structure analysis	0:17	Structure analysis of a novel heteroxylan from the stem of Dendrobium officinale and anti-angiogenesis activities of its sulfated derivative.	0:140	Structure analysis of a novel heteroxylan from the stem of Dendrobium officinale and anti-angiogenesis activities of its sulfated derivative.
28528944	5	25	from	position	998:1005	arg1	backbone					1031:1038	backbone	1031:1038	backbone	1031:1038	We further showed that it contained a backbone of 1,4-linked β-d-xylan, with branches of 1,4-linked α-d-glucose, 1,3-linked α-l-rhamnose, and terminal-linked α-l-arabinose, β-d-galactose, 4-MGA, and β-d-xylose directly or indirectly attached to C-2 position of glycosyl residues on backbone.
28528944	4	26	from	arabinose	593:601	arg1	ratio					713:717	a ratio	711:717	a ratio of 8.9:62.7:8.5:12.3:3.9:3.7	711:746	It composed of arabinose, xylose, glucose and 4-O-methylglucuronic acid (4-MGA) as well as trace amount of rhamnose and galactose in a ratio of 8.9:62.7:8.5:12.3:3.9:3.7.
28528944	5	27	theme	1,4-linked	799:808	arg1	β-d-xylan					810:818	1,4-linked β-d-xylan	799:818	1,4-linked β-d-xylan	799:818	We further showed that it contained a backbone of 1,4-linked β-d-xylan, with branches of 1,4-linked α-d-glucose, 1,3-linked α-l-rhamnose, and terminal-linked α-l-arabinose, β-d-galactose, 4-MGA, and β-d-xylose directly or indirectly attached to C-2 position of glycosyl residues on backbone.
28528944	7	28	theme	cells	1338:1342	arg1	migration					1293:1301	the migration	1289:1301	the migration of human microvascular endothelial cells (HMEC-1)	1289:1351	Bioactivity tests suggested that the sulfated polysaccharide could significantly disrupt tube formation and inhibit the migration of human microvascular endothelial cells (HMEC-1) at a low concentration (0.29μM) in a dose-dependent way without significant cytotoxity.
28528944	1	29	dep	Kimura	164:169	arg1	Migo					174:177	Migo	174:177	Migo	174:177	Dendrobium officinale Kimura et Migo (Tie-Pi-Shi-Hu), a precious folk medicine exhibiting multiple bioactivities, including antitumor, immune-enhancing and so on.
28528944	1	29	dep	Kimura	164:169	arg1	et					171:172	Kimura et Migo	164:177	Dendrobium officinale Kimura et Migo (Tie-Pi-Shi-Hu)	142:193	Dendrobium officinale Kimura et Migo (Tie-Pi-Shi-Hu), a precious folk medicine exhibiting multiple bioactivities, including antitumor, immune-enhancing and so on.
28528944	7	30	theme	human	1306:1310	arg1	HMEC-1					1345:1350	HMEC-1	1345:1350	HMEC-1	1345:1350	Bioactivity tests suggested that the sulfated polysaccharide could significantly disrupt tube formation and inhibit the migration of human microvascular endothelial cells (HMEC-1) at a low concentration (0.29μM) in a dose-dependent way without significant cytotoxity.
28528944	7	30	theme	human	1306:1310	arg1	cells					1338:1342	human microvascular endothelial cells	1306:1342	human microvascular endothelial cells (HMEC-1)	1306:1351	Bioactivity tests suggested that the sulfated polysaccharide could significantly disrupt tube formation and inhibit the migration of human microvascular endothelial cells (HMEC-1) at a low concentration (0.29μM) in a dose-dependent way without significant cytotoxity.
28528944	4	31	theme	trace	669:673	arg1	rhamnose					685:692	rhamnose	685:692	rhamnose	685:692	It composed of arabinose, xylose, glucose and 4-O-methylglucuronic acid (4-MGA) as well as trace amount of rhamnose and galactose in a ratio of 8.9:62.7:8.5:12.3:3.9:3.7.
28528944	4	31	theme	trace	669:673	arg1	amount					675:680	trace amount	669:680	trace amount of rhamnose and galactose in a ratio of 8.9:62.7:8.5:12.3:3.9:3.7	669:746	It composed of arabinose, xylose, glucose and 4-O-methylglucuronic acid (4-MGA) as well as trace amount of rhamnose and galactose in a ratio of 8.9:62.7:8.5:12.3:3.9:3.7.
28528944	4	31	theme	trace	669:673	arg1	galactose					698:706	galactose	698:706	galactose	698:706	It composed of arabinose, xylose, glucose and 4-O-methylglucuronic acid (4-MGA) as well as trace amount of rhamnose and galactose in a ratio of 8.9:62.7:8.5:12.3:3.9:3.7.
28528944	2	32	theme	fine	440:443	arg1	structures					445:454	fine structures	440:454	fine structures	440:454	Although evidences showed polysaccharide is one of the major bioactive substances from this herb, several of them were homogenous with fine structures elucidated.
28528944	5	33	attach	attached	982:989	arg2	branches					826:833	branches	826:833	branches of 1,4-linked α-d-glucose, 1,3-linked α-l-rhamnose, and terminal-linked α-l-arabinose, β-d-galactose, 4-MGA, and β-d-xylose directly or indirectly attached to C-2 position of glycosyl residues on backbone	826:1038	We further showed that it contained a backbone of 1,4-linked β-d-xylan, with branches of 1,4-linked α-d-glucose, 1,3-linked α-l-rhamnose, and terminal-linked α-l-arabinose, β-d-galactose, 4-MGA, and β-d-xylose directly or indirectly attached to C-2 position of glycosyl residues on backbone.
28528944	5	33	attach	attached	982:989	arg1	position					998:1005	C-2 position	994:1005	C-2 position of glycosyl residues on backbone	994:1038	We further showed that it contained a backbone of 1,4-linked β-d-xylan, with branches of 1,4-linked α-d-glucose, 1,3-linked α-l-rhamnose, and terminal-linked α-l-arabinose, β-d-galactose, 4-MGA, and β-d-xylose directly or indirectly attached to C-2 position of glycosyl residues on backbone.
28528944	5	34	link	1,3-linked	862:871	arg1	α-l-rhamnose					873:884	1,3-linked α-l-rhamnose	862:884	1,3-linked α-l-rhamnose	862:884	We further showed that it contained a backbone of 1,4-linked β-d-xylan, with branches of 1,4-linked α-d-glucose, 1,3-linked α-l-rhamnose, and terminal-linked α-l-arabinose, β-d-galactose, 4-MGA, and β-d-xylose directly or indirectly attached to C-2 position of glycosyl residues on backbone.
28528944	2	35	theme	evidences	314:322	arg1	polysaccharide					331:344	evidences showed polysaccharide	314:344	evidences showed polysaccharide	314:344	Although evidences showed polysaccharide is one of the major bioactive substances from this herb, several of them were homogenous with fine structures elucidated.
28528944	7	36	theme	Bioactivity	1173:1183	arg1	tests					1185:1189	Bioactivity tests	1173:1189	Bioactivity tests	1173:1189	Bioactivity tests suggested that the sulfated polysaccharide could significantly disrupt tube formation and inhibit the migration of human microvascular endothelial cells (HMEC-1) at a low concentration (0.29μM) in a dose-dependent way without significant cytotoxity.
28528944	5	37	theme	glycosyl	1010:1017	arg1	residues					1019:1026	glycosyl residues	1010:1026	glycosyl residues	1010:1026	We further showed that it contained a backbone of 1,4-linked β-d-xylan, with branches of 1,4-linked α-d-glucose, 1,3-linked α-l-rhamnose, and terminal-linked α-l-arabinose, β-d-galactose, 4-MGA, and β-d-xylose directly or indirectly attached to C-2 position of glycosyl residues on backbone.
28528944	0	38	theme	sulfated	121:128	arg1	derivative					130:139	its sulfated derivative	117:139	its sulfated derivative	117:139	Structure analysis of a novel heteroxylan from the stem of Dendrobium officinale and anti-angiogenesis activities of its sulfated derivative.
28528944	6	39	theme	-pyridine	1155:1163	arg1	method					1165:1170	the chlorosulfonic acid (CSA)-pyridine method	1126:1170	the chlorosulfonic acid (CSA)-pyridine method	1126:1170	The sulfated derivative with substitution degree about 0.9 was prepared according to the chlorosulfonic acid (CSA)-pyridine method.
28528944	6	40	theme	substitution	1070:1081	arg1	degree					1083:1088	substitution degree	1070:1088	substitution degree about 0.9	1070:1098	The sulfated derivative with substitution degree about 0.9 was prepared according to the chlorosulfonic acid (CSA)-pyridine method.
28528944	5	41	theme	β-d-xylose	948:957	arg1	branches					826:833	branches	826:833	branches of 1,4-linked α-d-glucose, 1,3-linked α-l-rhamnose, and terminal-linked α-l-arabinose, β-d-galactose, 4-MGA, and β-d-xylose directly or indirectly attached to C-2 position of glycosyl residues on backbone	826:1038	We further showed that it contained a backbone of 1,4-linked β-d-xylan, with branches of 1,4-linked α-d-glucose, 1,3-linked α-l-rhamnose, and terminal-linked α-l-arabinose, β-d-galactose, 4-MGA, and β-d-xylose directly or indirectly attached to C-2 position of glycosyl residues on backbone.
28528944	1	42	dep	officinale	153:162	arg1	Kimura					164:169	Kimura	164:169	Kimura	164:169	Dendrobium officinale Kimura et Migo (Tie-Pi-Shi-Hu), a precious folk medicine exhibiting multiple bioactivities, including antitumor, immune-enhancing and so on.
28528944	1	43	theme	Dendrobium	142:151	arg1	Tie-Pi-Shi-Hu					180:192	Tie-Pi-Shi-Hu	180:192	Tie-Pi-Shi-Hu	180:192	Dendrobium officinale Kimura et Migo (Tie-Pi-Shi-Hu), a precious folk medicine exhibiting multiple bioactivities, including antitumor, immune-enhancing and so on.
28528944	1	43	theme	Dendrobium	142:151	arg1	officinale					153:162	Dendrobium officinale	142:162	Dendrobium officinale Kimura et Migo (Tie-Pi-Shi-Hu)	142:193	Dendrobium officinale Kimura et Migo (Tie-Pi-Shi-Hu), a precious folk medicine exhibiting multiple bioactivities, including antitumor, immune-enhancing and so on.
28528944	1	43	theme	Dendrobium	142:151	arg1	medicine					212:219	a precious folk medicine	196:219	a precious folk medicine exhibiting multiple bioactivities, including antitumor, immune-enhancing and so on	196:302	Dendrobium officinale Kimura et Migo (Tie-Pi-Shi-Hu), a precious folk medicine exhibiting multiple bioactivities, including antitumor, immune-enhancing and so on.
28528944	0	44	theme	heteroxylan	30:40	arg1	analysis					10:17	Structure analysis	0:17	Structure analysis of a novel heteroxylan from the stem of Dendrobium officinale and anti-angiogenesis activities of its sulfated derivative.	0:140	Structure analysis of a novel heteroxylan from the stem of Dendrobium officinale and anti-angiogenesis activities of its sulfated derivative.
28528944	7	45	theme	endothelial	1326:1336	arg1	HMEC-1					1345:1350	HMEC-1	1345:1350	HMEC-1	1345:1350	Bioactivity tests suggested that the sulfated polysaccharide could significantly disrupt tube formation and inhibit the migration of human microvascular endothelial cells (HMEC-1) at a low concentration (0.29μM) in a dose-dependent way without significant cytotoxity.
28528944	7	45	theme	endothelial	1326:1336	arg1	cells					1338:1342	human microvascular endothelial cells	1306:1342	human microvascular endothelial cells (HMEC-1)	1306:1351	Bioactivity tests suggested that the sulfated polysaccharide could significantly disrupt tube formation and inhibit the migration of human microvascular endothelial cells (HMEC-1) at a low concentration (0.29μM) in a dose-dependent way without significant cytotoxity.
28528944	5	46	theme	1,4-linked	838:847	arg1	α-d-glucose					849:859	1,4-linked α-d-glucose	838:859	1,4-linked α-d-glucose	838:859	We further showed that it contained a backbone of 1,4-linked β-d-xylan, with branches of 1,4-linked α-d-glucose, 1,3-linked α-l-rhamnose, and terminal-linked α-l-arabinose, β-d-galactose, 4-MGA, and β-d-xylose directly or indirectly attached to C-2 position of glycosyl residues on backbone.
28528944	4	47	from	acid	645:648	arg1	ratio					713:717	a ratio	711:717	a ratio of 8.9:62.7:8.5:12.3:3.9:3.7	711:746	It composed of arabinose, xylose, glucose and 4-O-methylglucuronic acid (4-MGA) as well as trace amount of rhamnose and galactose in a ratio of 8.9:62.7:8.5:12.3:3.9:3.7.
28528944	1	48	theme	precious	198:205	arg1	officinale					153:162	Dendrobium officinale	142:162	Dendrobium officinale Kimura et Migo (Tie-Pi-Shi-Hu)	142:193	Dendrobium officinale Kimura et Migo (Tie-Pi-Shi-Hu), a precious folk medicine exhibiting multiple bioactivities, including antitumor, immune-enhancing and so on.
28528944	1	48	theme	precious	198:205	arg1	medicine					212:219	a precious folk medicine	196:219	a precious folk medicine exhibiting multiple bioactivities, including antitumor, immune-enhancing and so on	196:302	Dendrobium officinale Kimura et Migo (Tie-Pi-Shi-Hu), a precious folk medicine exhibiting multiple bioactivities, including antitumor, immune-enhancing and so on.
28528944	0	49	theme	novel	24:28	arg1	heteroxylan					30:40	a novel heteroxylan	22:40	a novel heteroxylan from the stem of Dendrobium officinale and anti-angiogenesis activities of its sulfated derivative	22:139	Structure analysis of a novel heteroxylan from the stem of Dendrobium officinale and anti-angiogenesis activities of its sulfated derivative.
28528944	0	50	theme	derivative	130:139	arg1	officinale					70:79	Dendrobium officinale and anti-angiogenesis activities	59:112	officinale	70:79	Structure analysis of a novel heteroxylan from the stem of Dendrobium officinale and anti-angiogenesis activities of its sulfated derivative.
28528944	0	50	theme	derivative	130:139	arg1	activities					103:112	Dendrobium officinale and anti-angiogenesis activities	59:112	activities	103:112	Structure analysis of a novel heteroxylan from the stem of Dendrobium officinale and anti-angiogenesis activities of its sulfated derivative.
28528944	4	51	theme	4-O-methylglucuronic	624:643	arg1	4-MGA					651:655	4-MGA	651:655	4-MGA	651:655	It composed of arabinose, xylose, glucose and 4-O-methylglucuronic acid (4-MGA) as well as trace amount of rhamnose and galactose in a ratio of 8.9:62.7:8.5:12.3:3.9:3.7.
28528944	4	51	theme	4-O-methylglucuronic	624:643	arg1	acid					645:648	4-O-methylglucuronic acid	624:648	4-O-methylglucuronic acid (4-MGA)	624:656	It composed of arabinose, xylose, glucose and 4-O-methylglucuronic acid (4-MGA) as well as trace amount of rhamnose and galactose in a ratio of 8.9:62.7:8.5:12.3:3.9:3.7.
28528944	5	52	theme	α-d-glucose	849:859	arg1	branches					826:833	branches	826:833	branches of 1,4-linked α-d-glucose, 1,3-linked α-l-rhamnose, and terminal-linked α-l-arabinose, β-d-galactose, 4-MGA, and β-d-xylose directly or indirectly attached to C-2 position of glycosyl residues on backbone	826:1038	We further showed that it contained a backbone of 1,4-linked β-d-xylan, with branches of 1,4-linked α-d-glucose, 1,3-linked α-l-rhamnose, and terminal-linked α-l-arabinose, β-d-galactose, 4-MGA, and β-d-xylose directly or indirectly attached to C-2 position of glycosyl residues on backbone.
28528944	1	53	theme	folk	207:210	arg1	officinale					153:162	Dendrobium officinale	142:162	Dendrobium officinale Kimura et Migo (Tie-Pi-Shi-Hu)	142:193	Dendrobium officinale Kimura et Migo (Tie-Pi-Shi-Hu), a precious folk medicine exhibiting multiple bioactivities, including antitumor, immune-enhancing and so on.
28528944	1	53	theme	folk	207:210	arg1	medicine					212:219	a precious folk medicine	196:219	a precious folk medicine exhibiting multiple bioactivities, including antitumor, immune-enhancing and so on	196:302	Dendrobium officinale Kimura et Migo (Tie-Pi-Shi-Hu), a precious folk medicine exhibiting multiple bioactivities, including antitumor, immune-enhancing and so on.
28528944	4	54	from	xylose	604:609	arg1	ratio					713:717	a ratio	711:717	a ratio of 8.9:62.7:8.5:12.3:3.9:3.7	711:746	It composed of arabinose, xylose, glucose and 4-O-methylglucuronic acid (4-MGA) as well as trace amount of rhamnose and galactose in a ratio of 8.9:62.7:8.5:12.3:3.9:3.7.
28528944	6	55	theme	sulfated	1045:1052	arg1	derivative					1054:1063	The sulfated derivative	1041:1063	The sulfated derivative with substitution degree about 0.9	1041:1098	The sulfated derivative with substitution degree about 0.9 was prepared according to the chlorosulfonic acid (CSA)-pyridine method.
28528944	0	56	from	stem	51:54	arg1	heteroxylan					30:40	a novel heteroxylan	22:40	a novel heteroxylan from the stem of Dendrobium officinale and anti-angiogenesis activities of its sulfated derivative	22:139	Structure analysis of a novel heteroxylan from the stem of Dendrobium officinale and anti-angiogenesis activities of its sulfated derivative.
28528944	0	56	from	stem	51:54	arg1	analysis					10:17	Structure analysis	0:17	Structure analysis of a novel heteroxylan from the stem of Dendrobium officinale and anti-angiogenesis activities of its sulfated derivative.	0:140	Structure analysis of a novel heteroxylan from the stem of Dendrobium officinale and anti-angiogenesis activities of its sulfated derivative.
28528944	2	57	with	homogenous	424:433	arg1	structures					445:454	fine structures	440:454	fine structures	440:454	Although evidences showed polysaccharide is one of the major bioactive substances from this herb, several of them were homogenous with fine structures elucidated.
28528944	5	58	theme	β-d-galactose	922:934	arg1	branches					826:833	branches	826:833	branches of 1,4-linked α-d-glucose, 1,3-linked α-l-rhamnose, and terminal-linked α-l-arabinose, β-d-galactose, 4-MGA, and β-d-xylose directly or indirectly attached to C-2 position of glycosyl residues on backbone	826:1038	We further showed that it contained a backbone of 1,4-linked β-d-xylan, with branches of 1,4-linked α-d-glucose, 1,3-linked α-l-rhamnose, and terminal-linked α-l-arabinose, β-d-galactose, 4-MGA, and β-d-xylose directly or indirectly attached to C-2 position of glycosyl residues on backbone.
28528944	5	59	link	terminal-linked	891:905	arg1	α-l-arabinose					907:919	terminal-linked α-l-arabinose	891:919	terminal-linked α-l-arabinose	891:919	We further showed that it contained a backbone of 1,4-linked β-d-xylan, with branches of 1,4-linked α-d-glucose, 1,3-linked α-l-rhamnose, and terminal-linked α-l-arabinose, β-d-galactose, 4-MGA, and β-d-xylose directly or indirectly attached to C-2 position of glycosyl residues on backbone.
28528944	7	60	theme	sulfated	1210:1217	arg1	polysaccharide					1219:1232	the sulfated polysaccharide	1206:1232	the sulfated polysaccharide	1206:1232	Bioactivity tests suggested that the sulfated polysaccharide could significantly disrupt tube formation and inhibit the migration of human microvascular endothelial cells (HMEC-1) at a low concentration (0.29μM) in a dose-dependent way without significant cytotoxity.
28528944	6	61	with	derivative	1054:1063	arg1	degree					1083:1088	substitution degree	1070:1088	substitution degree about 0.9	1070:1098	The sulfated derivative with substitution degree about 0.9 was prepared according to the chlorosulfonic acid (CSA)-pyridine method.
28528944	7	62	theme	dose-dependent	1390:1403	arg1	way					1405:1407	a dose-dependent way	1388:1407	a dose-dependent way without significant cytotoxity	1388:1438	Bioactivity tests suggested that the sulfated polysaccharide could significantly disrupt tube formation and inhibit the migration of human microvascular endothelial cells (HMEC-1) at a low concentration (0.29μM) in a dose-dependent way without significant cytotoxity.
28528944	4	63	theme	galactose	698:706	arg1	acid					645:648	4-O-methylglucuronic acid	624:648	4-O-methylglucuronic acid (4-MGA)	624:656	It composed of arabinose, xylose, glucose and 4-O-methylglucuronic acid (4-MGA) as well as trace amount of rhamnose and galactose in a ratio of 8.9:62.7:8.5:12.3:3.9:3.7.
28528944	4	63	theme	galactose	698:706	arg1	xylose					604:609	xylose	604:609	xylose	604:609	It composed of arabinose, xylose, glucose and 4-O-methylglucuronic acid (4-MGA) as well as trace amount of rhamnose and galactose in a ratio of 8.9:62.7:8.5:12.3:3.9:3.7.
28528944	4	63	theme	galactose	698:706	arg1	4-MGA					651:655	4-MGA	651:655	4-MGA	651:655	It composed of arabinose, xylose, glucose and 4-O-methylglucuronic acid (4-MGA) as well as trace amount of rhamnose and galactose in a ratio of 8.9:62.7:8.5:12.3:3.9:3.7.
28528944	4	63	theme	galactose	698:706	arg1	amount					675:680	trace amount	669:680	trace amount of rhamnose and galactose in a ratio of 8.9:62.7:8.5:12.3:3.9:3.7	669:746	It composed of arabinose, xylose, glucose and 4-O-methylglucuronic acid (4-MGA) as well as trace amount of rhamnose and galactose in a ratio of 8.9:62.7:8.5:12.3:3.9:3.7.
28528944	4	63	theme	galactose	698:706	arg1	rhamnose					685:692	rhamnose	685:692	rhamnose	685:692	It composed of arabinose, xylose, glucose and 4-O-methylglucuronic acid (4-MGA) as well as trace amount of rhamnose and galactose in a ratio of 8.9:62.7:8.5:12.3:3.9:3.7.
28528944	4	63	theme	galactose	698:706	arg1	glucose					612:618	glucose	612:618	glucose	612:618	It composed of arabinose, xylose, glucose and 4-O-methylglucuronic acid (4-MGA) as well as trace amount of rhamnose and galactose in a ratio of 8.9:62.7:8.5:12.3:3.9:3.7.
28528944	4	63	theme	galactose	698:706	arg1	arabinose					593:601	arabinose	593:601	arabinose	593:601	It composed of arabinose, xylose, glucose and 4-O-methylglucuronic acid (4-MGA) as well as trace amount of rhamnose and galactose in a ratio of 8.9:62.7:8.5:12.3:3.9:3.7.
28528944	4	63	theme	galactose	698:706	arg1	galactose					698:706	galactose	698:706	galactose	698:706	It composed of arabinose, xylose, glucose and 4-O-methylglucuronic acid (4-MGA) as well as trace amount of rhamnose and galactose in a ratio of 8.9:62.7:8.5:12.3:3.9:3.7.
28528944	2	64	from	herb	397:400	arg1	substances					376:385	the major bioactive substances	356:385	the major bioactive substances from this herb	356:400	Although evidences showed polysaccharide is one of the major bioactive substances from this herb, several of them were homogenous with fine structures elucidated.
28528944	5	65	theme	4-MGA	937:941	arg1	branches					826:833	branches	826:833	branches of 1,4-linked α-d-glucose, 1,3-linked α-l-rhamnose, and terminal-linked α-l-arabinose, β-d-galactose, 4-MGA, and β-d-xylose directly or indirectly attached to C-2 position of glycosyl residues on backbone	826:1038	We further showed that it contained a backbone of 1,4-linked β-d-xylan, with branches of 1,4-linked α-d-glucose, 1,3-linked α-l-rhamnose, and terminal-linked α-l-arabinose, β-d-galactose, 4-MGA, and β-d-xylose directly or indirectly attached to C-2 position of glycosyl residues on backbone.
28528944	7	66	theme	significant	1417:1427	arg1	cytotoxity					1429:1438	significant cytotoxity	1417:1438	significant cytotoxity	1417:1438	Bioactivity tests suggested that the sulfated polysaccharide could significantly disrupt tube formation and inhibit the migration of human microvascular endothelial cells (HMEC-1) at a low concentration (0.29μM) in a dose-dependent way without significant cytotoxity.
28528944	5	67	theme	C-2	994:996	arg1	position					998:1005	C-2 position	994:1005	C-2 position of glycosyl residues on backbone	994:1038	We further showed that it contained a backbone of 1,4-linked β-d-xylan, with branches of 1,4-linked α-d-glucose, 1,3-linked α-l-rhamnose, and terminal-linked α-l-arabinose, β-d-galactose, 4-MGA, and β-d-xylose directly or indirectly attached to C-2 position of glycosyl residues on backbone.
28528944	5	68	theme	terminal-linked	891:905	arg1	α-l-arabinose					907:919	terminal-linked α-l-arabinose	891:919	terminal-linked α-l-arabinose	891:919	We further showed that it contained a backbone of 1,4-linked β-d-xylan, with branches of 1,4-linked α-d-glucose, 1,3-linked α-l-rhamnose, and terminal-linked α-l-arabinose, β-d-galactose, 4-MGA, and β-d-xylose directly or indirectly attached to C-2 position of glycosyl residues on backbone.
28528944	6	69	theme	acid	1145:1148	arg1	method					1165:1170	the chlorosulfonic acid (CSA)-pyridine method	1126:1170	the chlorosulfonic acid (CSA)-pyridine method	1126:1170	The sulfated derivative with substitution degree about 0.9 was prepared according to the chlorosulfonic acid (CSA)-pyridine method.
28528944	7	70	theme	tube	1262:1265	arg1	formation					1267:1275	tube formation	1262:1275	tube formation	1262:1275	Bioactivity tests suggested that the sulfated polysaccharide could significantly disrupt tube formation and inhibit the migration of human microvascular endothelial cells (HMEC-1) at a low concentration (0.29μM) in a dose-dependent way without significant cytotoxity.
28528944	5	71	contain	contained	775:783	arg2	backbone					787:794	a backbone	785:794	a backbone of 1,4-linked β-d-xylan	785:818	We further showed that it contained a backbone of 1,4-linked β-d-xylan, with branches of 1,4-linked α-d-glucose, 1,3-linked α-l-rhamnose, and terminal-linked α-l-arabinose, β-d-galactose, 4-MGA, and β-d-xylose directly or indirectly attached to C-2 position of glycosyl residues on backbone.
28528944	5	71	contain	contained	775:783	arg1	it					772:773	it	772:773	it	772:773	We further showed that it contained a backbone of 1,4-linked β-d-xylan, with branches of 1,4-linked α-d-glucose, 1,3-linked α-l-rhamnose, and terminal-linked α-l-arabinose, β-d-galactose, 4-MGA, and β-d-xylose directly or indirectly attached to C-2 position of glycosyl residues on backbone.
28528944	5	72	link	1,4-linked	799:808	arg1	β-d-xylan					810:818	1,4-linked β-d-xylan	799:818	1,4-linked β-d-xylan	799:818	We further showed that it contained a backbone of 1,4-linked β-d-xylan, with branches of 1,4-linked α-d-glucose, 1,3-linked α-l-rhamnose, and terminal-linked α-l-arabinose, β-d-galactose, 4-MGA, and β-d-xylose directly or indirectly attached to C-2 position of glycosyl residues on backbone.
28528944	2	73	theme	substances	376:385	arg1	one					349:351	one	349:351	one	349:351	Although evidences showed polysaccharide is one of the major bioactive substances from this herb, several of them were homogenous with fine structures elucidated.
28528944	2	73	theme	substances	376:385	arg1	substances					376:385	the major bioactive substances	356:385	the major bioactive substances from this herb	356:400	Although evidences showed polysaccharide is one of the major bioactive substances from this herb, several of them were homogenous with fine structures elucidated.
28528944	5	74	theme	1,3-linked	862:871	arg1	α-l-rhamnose					873:884	1,3-linked α-l-rhamnose	862:884	1,3-linked α-l-rhamnose	862:884	We further showed that it contained a backbone of 1,4-linked β-d-xylan, with branches of 1,4-linked α-d-glucose, 1,3-linked α-l-rhamnose, and terminal-linked α-l-arabinose, β-d-galactose, 4-MGA, and β-d-xylose directly or indirectly attached to C-2 position of glycosyl residues on backbone.
28528944	1	75	theme	multiple	232:239	arg1	so					298:299	so	298:299	so	298:299	Dendrobium officinale Kimura et Migo (Tie-Pi-Shi-Hu), a precious folk medicine exhibiting multiple bioactivities, including antitumor, immune-enhancing and so on.
28528944	1	75	theme	multiple	232:239	arg1	immune-enhancing					277:292	immune-enhancing	277:292	immune-enhancing	277:292	Dendrobium officinale Kimura et Migo (Tie-Pi-Shi-Hu), a precious folk medicine exhibiting multiple bioactivities, including antitumor, immune-enhancing and so on.
28528944	1	75	theme	multiple	232:239	arg1	bioactivities					241:253	multiple bioactivities	232:253	multiple bioactivities	232:253	Dendrobium officinale Kimura et Migo (Tie-Pi-Shi-Hu), a precious folk medicine exhibiting multiple bioactivities, including antitumor, immune-enhancing and so on.
28528944	1	75	theme	multiple	232:239	arg1	antitumor					266:274	antitumor	266:274	antitumor	266:274	Dendrobium officinale Kimura et Migo (Tie-Pi-Shi-Hu), a precious folk medicine exhibiting multiple bioactivities, including antitumor, immune-enhancing and so on.
28528944	5	76	theme	residues	1019:1026	arg1	position					998:1005	C-2 position	994:1005	C-2 position of glycosyl residues on backbone	994:1038	We further showed that it contained a backbone of 1,4-linked β-d-xylan, with branches of 1,4-linked α-d-glucose, 1,3-linked α-l-rhamnose, and terminal-linked α-l-arabinose, β-d-galactose, 4-MGA, and β-d-xylose directly or indirectly attached to C-2 position of glycosyl residues on backbone.
28528944	2	77	theme	bioactive	366:374	arg1	substances					376:385	the major bioactive substances	356:385	the major bioactive substances from this herb	356:400	Although evidences showed polysaccharide is one of the major bioactive substances from this herb, several of them were homogenous with fine structures elucidated.
28950882	8	0	theme	epsB	1617:1620	arg1	strain					1634:1639	the epsB recombinant strain	1613:1639	the epsB recombinant strain	1613:1639	The bioflocculant production of the epsB recombinant strain was further evaluated during batch fermentation in a 2 L fermenter; the flocculating activity reached 9612.75 U/mL, and the bioflocculant yield reached 10.26 g/L after 72 h, representing increases of 224% and 36.62%, respectively, compared with the original strain.
28950882	2	1	theme	CGMCC	387:391	arg1	2876					393:396	Bacillus licheniformis CGMCC 2876	364:396	Bacillus licheniformis CGMCC 2876	364:396	Bacillus licheniformis CGMCC 2876 can produce polysaccharide and γ-PGA bioflocculants under different culture conditions.
28950882	6	2	theme	%	1230:1230	arg1	protein					1232:1238	3.464% protein	1225:1238	3.464% protein	1225:1238	The composition of the bioflocculant from the epsB recombinant strain was 28.95% total sugar, 3.464% protein and 44.03% γ-PGA, while in the original strain, these components represented 53.67%, 3.246% and 34.13%, respectively.
28950882	8	3	theme	strain	1634:1639	arg1	production					1599:1608	The bioflocculant production	1581:1608	The bioflocculant production of the epsB recombinant strain	1581:1639	The bioflocculant production of the epsB recombinant strain was further evaluated during batch fermentation in a 2 L fermenter; the flocculating activity reached 9612.75 U/mL, and the bioflocculant yield reached 10.26 g/L after 72 h, representing increases of 224% and 36.62%, respectively, compared with the original strain.
28950882	6	4	from	composition	1135:1145	arg1	strain					1194:1199	the epsB recombinant strain	1173:1199	the epsB recombinant strain	1173:1199	The composition of the bioflocculant from the epsB recombinant strain was 28.95% total sugar, 3.464% protein and 44.03% γ-PGA, while in the original strain, these components represented 53.67%, 3.246% and 34.13%, respectively.
28950882	7	5	theme	several	1423:1429	arg1	genes					1435:1439	several key genes	1423:1439	several key genes involved in γ-PGA synthesis in B. licheniformis	1423:1487	In combination with an analysis of the transcriptional levels of several key genes involved in γ-PGA synthesis in B. licheniformis, we inferred that epsB played a key role in the synthesis of both polysaccharide and γ-PGA.
28950882	9	6	theme	epsA	2130:2133	arg1	overexpression					2112:2125	the overexpression	2108:2125	the overexpression of epsA	2108:2133	Moreover, we found that the tandem expression of phosphoglucomutase (pgcA) and UTP-glucose-1-phosphate uridylyltransferase (gtaB1) could enhance the crude yield of the bioflocculant by 20.77% and that the overexpression of epsA could enhance the bioflocculant yield by 23.70% compared with the original strain.
28950882	1	7	theme	poly-γ-glutamic	193:207	arg1	acid					209:212	poly-γ-glutamic acid	193:212	poly-γ-glutamic acid (γ-PGA)	193:220	BACKGROUND Polysaccharides and poly-γ-glutamic acid (γ-PGA) are biomacromolecules that have been reported as bioflocculants, and they exhibit high flocculating activity in many industrial applications.
28950882	1	7	theme	poly-γ-glutamic	193:207	arg1	Polysaccharides					173:187	BACKGROUND Polysaccharides	162:187	BACKGROUND Polysaccharides	162:187	BACKGROUND Polysaccharides and poly-γ-glutamic acid (γ-PGA) are biomacromolecules that have been reported as bioflocculants, and they exhibit high flocculating activity in many industrial applications.
28950882	1	7	theme	poly-γ-glutamic	193:207	arg1	γ-PGA					215:219	γ-PGA	215:219	γ-PGA	215:219	BACKGROUND Polysaccharides and poly-γ-glutamic acid (γ-PGA) are biomacromolecules that have been reported as bioflocculants, and they exhibit high flocculating activity in many industrial applications.
28950882	1	7	theme	poly-γ-glutamic	193:207	arg1	biomacromolecules					226:242	biomacromolecules	226:242	biomacromolecules that have been reported as bioflocculants	226:284	BACKGROUND Polysaccharides and poly-γ-glutamic acid (γ-PGA) are biomacromolecules that have been reported as bioflocculants, and they exhibit high flocculating activity in many industrial applications.
28950882	7	8	theme	γ-PGA	1453:1457	arg1	synthesis					1459:1467	γ-PGA synthesis	1453:1467	γ-PGA synthesis in B. licheniformis	1453:1487	In combination with an analysis of the transcriptional levels of several key genes involved in γ-PGA synthesis in B. licheniformis, we inferred that epsB played a key role in the synthesis of both polysaccharide and γ-PGA.
28950882	6	9	theme	total	1212:1216	arg1	sugar					1218:1222	28.95% total sugar	1205:1222	28.95% total sugar	1205:1222	The composition of the bioflocculant from the epsB recombinant strain was 28.95% total sugar, 3.464% protein and 44.03% γ-PGA, while in the original strain, these components represented 53.67%, 3.246% and 34.13%, respectively.
28950882	6	9	theme	total	1212:1216	arg1	composition					1135:1145	The composition	1131:1145	The composition of the bioflocculant from the epsB recombinant strain	1131:1199	The composition of the bioflocculant from the epsB recombinant strain was 28.95% total sugar, 3.464% protein and 44.03% γ-PGA, while in the original strain, these components represented 53.67%, 3.246% and 34.13%, respectively.
28950882	7	10	from	synthesis	1459:1467	arg1	licheniformis					1475:1487	B. licheniformis	1472:1487	B. licheniformis	1472:1487	In combination with an analysis of the transcriptional levels of several key genes involved in γ-PGA synthesis in B. licheniformis, we inferred that epsB played a key role in the synthesis of both polysaccharide and γ-PGA.
28950882	6	11	theme	28.95	1205:1209	arg1	%					1210:1210	%	1210:1210	%	1210:1210	The composition of the bioflocculant from the epsB recombinant strain was 28.95% total sugar, 3.464% protein and 44.03% γ-PGA, while in the original strain, these components represented 53.67%, 3.246% and 34.13%, respectively.
28950882	9	12	theme	bioflocculant	2153:2165	arg1	yield					2167:2171	the bioflocculant yield	2149:2171	the bioflocculant yield by 23.70%	2149:2181	Moreover, we found that the tandem expression of phosphoglucomutase (pgcA) and UTP-glucose-1-phosphate uridylyltransferase (gtaB1) could enhance the crude yield of the bioflocculant by 20.77% and that the overexpression of epsA could enhance the bioflocculant yield by 23.70% compared with the original strain.
28950882	8	13	theme	original	1890:1897	arg1	strain					1899:1904	the original strain	1886:1904	the original strain	1886:1904	The bioflocculant production of the epsB recombinant strain was further evaluated during batch fermentation in a 2 L fermenter; the flocculating activity reached 9612.75 U/mL, and the bioflocculant yield reached 10.26 g/L after 72 h, representing increases of 224% and 36.62%, respectively, compared with the original strain.
28950882	3	14	theme	regulation	605:614	arg1	impacts					590:596	the impacts	586:596	the impacts of the regulation of these genes on the production of polysaccharide bioflocculants	586:680	Several key genes are involved in the metabolic pathway of polysaccharides in B. licheniformis, but the impacts of the regulation of these genes on the production of polysaccharide bioflocculants have not been illustrated completely.
28950882	9	15	theme	tandem	1935:1940	arg1	expression					1942:1951	the tandem expression	1931:1951	the tandem expression of phosphoglucomutase (pgcA) and UTP-glucose-1-phosphate uridylyltransferase (gtaB1)	1931:2036	Moreover, we found that the tandem expression of phosphoglucomutase (pgcA) and UTP-glucose-1-phosphate uridylyltransferase (gtaB1) could enhance the crude yield of the bioflocculant by 20.77% and that the overexpression of epsA could enhance the bioflocculant yield by 23.70% compared with the original strain.
28950882	5	16	theme	flocculating	1097:1108	arg1	activity					1110:1117	the flocculating activity	1093:1117	the flocculating activity	1093:1117	RESULTS Overexpressing epsB from the eps gene cluster not only improved the bioflocculant crude yield by 13.98% but also enhanced the flocculating activity by 117.92%.
28950882	8	17	theme	bioflocculant	1765:1777	arg1	yield					1779:1783	the bioflocculant yield	1761:1783	the bioflocculant yield	1761:1783	The bioflocculant production of the epsB recombinant strain was further evaluated during batch fermentation in a 2 L fermenter; the flocculating activity reached 9612.75 U/mL, and the bioflocculant yield reached 10.26 g/L after 72 h, representing increases of 224% and 36.62%, respectively, compared with the original strain.
28950882	10	18	dep	CONCLUSIONS	2218:2228	arg1	provides					2241:2248	provides	2241:2248	provides a new method to greatly increase the bioflocculant production in B. licheniformis	2241:2330	CONCLUSIONS This study provides a new method to greatly increase the bioflocculant production in B. licheniformis, and it demonstrates the correlation between the biosynthesis of polysaccharide and γ-PGA during EPS fermentation by regulating the expression of EpsB.
28950882	10	18	dep	CONCLUSIONS	2218:2228	arg1	demonstrates					2340:2351	demonstrates	2340:2351	demonstrates the correlation between the biosynthesis of polysaccharide and γ-PGA during EPS fermentation by regulating the expression of EpsB	2340:2481	CONCLUSIONS This study provides a new method to greatly increase the bioflocculant production in B. licheniformis, and it demonstrates the correlation between the biosynthesis of polysaccharide and γ-PGA during EPS fermentation by regulating the expression of EpsB.
28950882	6	19	theme	%	1249:1249	arg1	γ-PGA					1251:1255	44.03% γ-PGA	1244:1255	44.03% γ-PGA	1244:1255	The composition of the bioflocculant from the epsB recombinant strain was 28.95% total sugar, 3.464% protein and 44.03% γ-PGA, while in the original strain, these components represented 53.67%, 3.246% and 34.13%, respectively.
28950882	7	20	theme	genes	1435:1439	arg1	levels					1413:1418	the transcriptional levels	1393:1418	the transcriptional levels of several key genes involved in γ-PGA synthesis in B. licheniformis	1393:1487	In combination with an analysis of the transcriptional levels of several key genes involved in γ-PGA synthesis in B. licheniformis, we inferred that epsB played a key role in the synthesis of both polysaccharide and γ-PGA.
28950882	5	21	theme	eps	1000:1002	arg1	cluster					1009:1015	the eps gene cluster	996:1015	the eps gene cluster	996:1015	RESULTS Overexpressing epsB from the eps gene cluster not only improved the bioflocculant crude yield by 13.98% but also enhanced the flocculating activity by 117.92%.
28950882	9	22	theme	original	2201:2208	arg1	strain					2210:2215	the original strain	2197:2215	the original strain	2197:2215	Moreover, we found that the tandem expression of phosphoglucomutase (pgcA) and UTP-glucose-1-phosphate uridylyltransferase (gtaB1) could enhance the crude yield of the bioflocculant by 20.77% and that the overexpression of epsA could enhance the bioflocculant yield by 23.70% compared with the original strain.
28950882	3	23	theme	polysaccharide	652:665	arg1	bioflocculants					667:680	polysaccharide bioflocculants	652:680	polysaccharide bioflocculants	652:680	Several key genes are involved in the metabolic pathway of polysaccharides in B. licheniformis, but the impacts of the regulation of these genes on the production of polysaccharide bioflocculants have not been illustrated completely.
28950882	7	24	with	combination	1361:1371	arg1	analysis					1381:1388	an analysis	1378:1388	an analysis of the transcriptional levels of several key genes involved in γ-PGA synthesis in B. licheniformis	1378:1487	In combination with an analysis of the transcriptional levels of several key genes involved in γ-PGA synthesis in B. licheniformis, we inferred that epsB played a key role in the synthesis of both polysaccharide and γ-PGA.
28950882	10	25	theme	polysaccharide	2397:2410	arg1	biosynthesis					2381:2392	the biosynthesis	2377:2392	the biosynthesis of polysaccharide and γ-PGA	2377:2420	CONCLUSIONS This study provides a new method to greatly increase the bioflocculant production in B. licheniformis, and it demonstrates the correlation between the biosynthesis of polysaccharide and γ-PGA during EPS fermentation by regulating the expression of EpsB.
28950882	9	26	theme	UTP-glucose-1-phosphate	1986:2008	arg1	uridylyltransferase					2010:2028	UTP-glucose-1-phosphate uridylyltransferase	1986:2028	UTP-glucose-1-phosphate uridylyltransferase (gtaB1)	1986:2036	Moreover, we found that the tandem expression of phosphoglucomutase (pgcA) and UTP-glucose-1-phosphate uridylyltransferase (gtaB1) could enhance the crude yield of the bioflocculant by 20.77% and that the overexpression of epsA could enhance the bioflocculant yield by 23.70% compared with the original strain.
28950882	9	26	theme	UTP-glucose-1-phosphate	1986:2008	arg1	gtaB1					2031:2035	gtaB1	2031:2035	gtaB1	2031:2035	Moreover, we found that the tandem expression of phosphoglucomutase (pgcA) and UTP-glucose-1-phosphate uridylyltransferase (gtaB1) could enhance the crude yield of the bioflocculant by 20.77% and that the overexpression of epsA could enhance the bioflocculant yield by 23.70% compared with the original strain.
28950882	3	27	theme	key	494:496	arg1	genes					498:502	Several key genes	486:502	Several key genes	486:502	Several key genes are involved in the metabolic pathway of polysaccharides in B. licheniformis, but the impacts of the regulation of these genes on the production of polysaccharide bioflocculants have not been illustrated completely.
28950882	5	28	theme	crude	1053:1057	arg1	yield					1059:1063	the bioflocculant crude yield	1035:1063	the bioflocculant crude yield	1035:1063	RESULTS Overexpressing epsB from the eps gene cluster not only improved the bioflocculant crude yield by 13.98% but also enhanced the flocculating activity by 117.92%.
28950882	10	29	theme	EPS	2429:2431	arg1	fermentation					2433:2444	EPS fermentation	2429:2444	EPS fermentation	2429:2444	CONCLUSIONS This study provides a new method to greatly increase the bioflocculant production in B. licheniformis, and it demonstrates the correlation between the biosynthesis of polysaccharide and γ-PGA during EPS fermentation by regulating the expression of EpsB.
28950882	10	30	theme	γ-PGA	2416:2420	arg1	biosynthesis					2381:2392	the biosynthesis	2377:2392	the biosynthesis of polysaccharide and γ-PGA	2377:2420	CONCLUSIONS This study provides a new method to greatly increase the bioflocculant production in B. licheniformis, and it demonstrates the correlation between the biosynthesis of polysaccharide and γ-PGA during EPS fermentation by regulating the expression of EpsB.
28950882	0	31	theme	bioflocculant	15:27	arg1	production					29:38	the bioflocculant production	11:38	the bioflocculant production	11:38	Increasing the bioflocculant production and identifying the effect of overexpressing epsB on the synthesis of polysaccharide and γ-PGA in Bacillus licheniformis.
28950882	1	32	theme	high	304:307	arg1	activity					322:329	high flocculating activity	304:329	high flocculating activity in many industrial applications	304:361	BACKGROUND Polysaccharides and poly-γ-glutamic acid (γ-PGA) are biomacromolecules that have been reported as bioflocculants, and they exhibit high flocculating activity in many industrial applications.
28950882	9	33	theme	crude	2056:2060	arg1	yield					2062:2066	the crude yield	2052:2066	the crude yield of the bioflocculant	2052:2087	Moreover, we found that the tandem expression of phosphoglucomutase (pgcA) and UTP-glucose-1-phosphate uridylyltransferase (gtaB1) could enhance the crude yield of the bioflocculant by 20.77% and that the overexpression of epsA could enhance the bioflocculant yield by 23.70% compared with the original strain.
28950882	2	34	theme	different	456:464	arg1	conditions					474:483	different culture conditions	456:483	different culture conditions	456:483	Bacillus licheniformis CGMCC 2876 can produce polysaccharide and γ-PGA bioflocculants under different culture conditions.
28950882	2	35	theme	licheniformis	373:385	arg1	2876					393:396	Bacillus licheniformis CGMCC 2876	364:396	Bacillus licheniformis CGMCC 2876	364:396	Bacillus licheniformis CGMCC 2876 can produce polysaccharide and γ-PGA bioflocculants under different culture conditions.
28950882	1	36	theme	many	334:337	arg1	applications					350:361	many industrial applications	334:361	many industrial applications	334:361	BACKGROUND Polysaccharides and poly-γ-glutamic acid (γ-PGA) are biomacromolecules that have been reported as bioflocculants, and they exhibit high flocculating activity in many industrial applications.
28950882	6	37	theme	recombinant	1182:1192	arg1	strain					1194:1199	the epsB recombinant strain	1173:1199	the epsB recombinant strain	1173:1199	The composition of the bioflocculant from the epsB recombinant strain was 28.95% total sugar, 3.464% protein and 44.03% γ-PGA, while in the original strain, these components represented 53.67%, 3.246% and 34.13%, respectively.
28950882	9	38	theme	bioflocculant	2075:2087	arg1	yield					2062:2066	the crude yield	2052:2066	the crude yield of the bioflocculant	2052:2087	Moreover, we found that the tandem expression of phosphoglucomutase (pgcA) and UTP-glucose-1-phosphate uridylyltransferase (gtaB1) could enhance the crude yield of the bioflocculant by 20.77% and that the overexpression of epsA could enhance the bioflocculant yield by 23.70% compared with the original strain.
28950882	3	39	theme	metabolic	524:532	arg1	pathway					534:540	the metabolic pathway	520:540	the metabolic pathway of polysaccharides in B. licheniformis	520:579	Several key genes are involved in the metabolic pathway of polysaccharides in B. licheniformis, but the impacts of the regulation of these genes on the production of polysaccharide bioflocculants have not been illustrated completely.
28950882	4	40	theme	key	868:870	arg1	genes					872:876	a few key genes	862:876	a few key genes	862:876	To increase the bioflocculant production and identify the correlation between the synthesis of polysaccharides and γ-PGA in B. licheniformis, a few key genes were investigated to explore their influence on the synthesis of the bioflocculants.
28950882	6	41	theme	bioflocculant	1154:1166	arg1	sugar					1218:1222	28.95% total sugar	1205:1222	28.95% total sugar	1205:1222	The composition of the bioflocculant from the epsB recombinant strain was 28.95% total sugar, 3.464% protein and 44.03% γ-PGA, while in the original strain, these components represented 53.67%, 3.246% and 34.13%, respectively.
28950882	6	41	theme	bioflocculant	1154:1166	arg1	composition					1135:1145	The composition	1131:1145	The composition of the bioflocculant from the epsB recombinant strain	1131:1199	The composition of the bioflocculant from the epsB recombinant strain was 28.95% total sugar, 3.464% protein and 44.03% γ-PGA, while in the original strain, these components represented 53.67%, 3.246% and 34.13%, respectively.
28950882	8	42	theme	%	1855:1855	arg1	increases					1828:1836	increases	1828:1836	increases of 224% and 36.62%, respectively	1828:1869	The bioflocculant production of the epsB recombinant strain was further evaluated during batch fermentation in a 2 L fermenter; the flocculating activity reached 9612.75 U/mL, and the bioflocculant yield reached 10.26 g/L after 72 h, representing increases of 224% and 36.62%, respectively, compared with the original strain.
28950882	8	43	theme	batch	1670:1674	arg1	fermentation					1676:1687	batch fermentation	1670:1687	batch fermentation	1670:1687	The bioflocculant production of the epsB recombinant strain was further evaluated during batch fermentation in a 2 L fermenter; the flocculating activity reached 9612.75 U/mL, and the bioflocculant yield reached 10.26 g/L after 72 h, representing increases of 224% and 36.62%, respectively, compared with the original strain.
28950882	10	44	theme	new	2252:2254	arg1	method					2256:2261	a new method	2250:2261	a new method to greatly increase the bioflocculant production in B. licheniformis	2250:2330	CONCLUSIONS This study provides a new method to greatly increase the bioflocculant production in B. licheniformis, and it demonstrates the correlation between the biosynthesis of polysaccharide and γ-PGA during EPS fermentation by regulating the expression of EpsB.
28950882	1	45	from	activity	322:329	arg1	applications					350:361	many industrial applications	334:361	many industrial applications	334:361	BACKGROUND Polysaccharides and poly-γ-glutamic acid (γ-PGA) are biomacromolecules that have been reported as bioflocculants, and they exhibit high flocculating activity in many industrial applications.
28950882	7	46	theme	levels	1413:1418	arg1	analysis					1381:1388	an analysis	1378:1388	an analysis of the transcriptional levels of several key genes involved in γ-PGA synthesis in B. licheniformis	1378:1487	In combination with an analysis of the transcriptional levels of several key genes involved in γ-PGA synthesis in B. licheniformis, we inferred that epsB played a key role in the synthesis of both polysaccharide and γ-PGA.
28950882	6	47	theme	3.464	1225:1229	arg1	%					1230:1230	%	1230:1230	%	1230:1230	The composition of the bioflocculant from the epsB recombinant strain was 28.95% total sugar, 3.464% protein and 44.03% γ-PGA, while in the original strain, these components represented 53.67%, 3.246% and 34.13%, respectively.
28950882	8	48	theme	recombinant	1622:1632	arg1	strain					1634:1639	the epsB recombinant strain	1613:1639	the epsB recombinant strain	1613:1639	The bioflocculant production of the epsB recombinant strain was further evaluated during batch fermentation in a 2 L fermenter; the flocculating activity reached 9612.75 U/mL, and the bioflocculant yield reached 10.26 g/L after 72 h, representing increases of 224% and 36.62%, respectively, compared with the original strain.
28950882	8	49	theme	2 L	1694:1696	arg1	fermenter					1698:1706	a 2 L fermenter	1692:1706	a 2 L fermenter	1692:1706	The bioflocculant production of the epsB recombinant strain was further evaluated during batch fermentation in a 2 L fermenter; the flocculating activity reached 9612.75 U/mL, and the bioflocculant yield reached 10.26 g/L after 72 h, representing increases of 224% and 36.62%, respectively, compared with the original strain.
28950882	7	50	theme	key	1431:1433	arg1	genes					1435:1439	several key genes	1423:1439	several key genes involved in γ-PGA synthesis in B. licheniformis	1423:1487	In combination with an analysis of the transcriptional levels of several key genes involved in γ-PGA synthesis in B. licheniformis, we inferred that epsB played a key role in the synthesis of both polysaccharide and γ-PGA.
28950882	10	51	theme	EpsB	2478:2481	arg1	expression					2464:2473	the expression	2460:2473	the expression of EpsB	2460:2481	CONCLUSIONS This study provides a new method to greatly increase the bioflocculant production in B. licheniformis, and it demonstrates the correlation between the biosynthesis of polysaccharide and γ-PGA during EPS fermentation by regulating the expression of EpsB.
28950882	0	52	theme	epsB	85:88	arg1	effect					60:65	the effect	56:65	the effect of overexpressing epsB on the synthesis of polysaccharide and γ-PGA in Bacillus licheniformis	56:159	Increasing the bioflocculant production and identifying the effect of overexpressing epsB on the synthesis of polysaccharide and γ-PGA in Bacillus licheniformis.
28950882	6	53	theme	original	1271:1278	arg1	strain					1280:1285	the original strain	1267:1285	the original strain	1267:1285	The composition of the bioflocculant from the epsB recombinant strain was 28.95% total sugar, 3.464% protein and 44.03% γ-PGA, while in the original strain, these components represented 53.67%, 3.246% and 34.13%, respectively.
28950882	3	54	from	pathway	534:540	arg1	licheniformis					567:579	B. licheniformis	564:579	B. licheniformis	564:579	Several key genes are involved in the metabolic pathway of polysaccharides in B. licheniformis, but the impacts of the regulation of these genes on the production of polysaccharide bioflocculants have not been illustrated completely.
28950882	6	55	theme	%	1210:1210	arg1	sugar					1218:1222	28.95% total sugar	1205:1222	28.95% total sugar	1205:1222	The composition of the bioflocculant from the epsB recombinant strain was 28.95% total sugar, 3.464% protein and 44.03% γ-PGA, while in the original strain, these components represented 53.67%, 3.246% and 34.13%, respectively.
28950882	6	55	theme	%	1210:1210	arg1	composition					1135:1145	The composition	1131:1145	The composition of the bioflocculant from the epsB recombinant strain	1131:1199	The composition of the bioflocculant from the epsB recombinant strain was 28.95% total sugar, 3.464% protein and 44.03% γ-PGA, while in the original strain, these components represented 53.67%, 3.246% and 34.13%, respectively.
28950882	0	56	theme	γ-PGA	129:133	arg1	synthesis					97:105	the synthesis	93:105	the synthesis of polysaccharide and γ-PGA in Bacillus licheniformis	93:159	Increasing the bioflocculant production and identifying the effect of overexpressing epsB on the synthesis of polysaccharide and γ-PGA in Bacillus licheniformis.
28950882	8	57	theme	flocculating	1713:1724	arg1	activity					1726:1733	the flocculating activity	1709:1733	the flocculating activity	1709:1733	The bioflocculant production of the epsB recombinant strain was further evaluated during batch fermentation in a 2 L fermenter; the flocculating activity reached 9612.75 U/mL, and the bioflocculant yield reached 10.26 g/L after 72 h, representing increases of 224% and 36.62%, respectively, compared with the original strain.
28950882	7	58	theme	transcriptional	1397:1411	arg1	levels					1413:1418	the transcriptional levels	1393:1418	the transcriptional levels of several key genes involved in γ-PGA synthesis in B. licheniformis	1393:1487	In combination with an analysis of the transcriptional levels of several key genes involved in γ-PGA synthesis in B. licheniformis, we inferred that epsB played a key role in the synthesis of both polysaccharide and γ-PGA.
28950882	2	59	theme	Bacillus	364:371	arg1	2876					393:396	Bacillus licheniformis CGMCC 2876	364:396	Bacillus licheniformis CGMCC 2876	364:396	Bacillus licheniformis CGMCC 2876 can produce polysaccharide and γ-PGA bioflocculants under different culture conditions.
28950882	10	60	theme	bioflocculant	2287:2299	arg1	production					2301:2310	the bioflocculant production	2283:2310	the bioflocculant production in B. licheniformis	2283:2330	CONCLUSIONS This study provides a new method to greatly increase the bioflocculant production in B. licheniformis, and it demonstrates the correlation between the biosynthesis of polysaccharide and γ-PGA during EPS fermentation by regulating the expression of EpsB.
28950882	3	61	theme	genes	625:629	arg1	regulation					605:614	the regulation	601:614	the regulation of these genes	601:629	Several key genes are involved in the metabolic pathway of polysaccharides in B. licheniformis, but the impacts of the regulation of these genes on the production of polysaccharide bioflocculants have not been illustrated completely.
28950882	4	62	theme	bioflocculants	947:960	arg1	synthesis					930:938	the synthesis	926:938	the synthesis of the bioflocculants	926:960	To increase the bioflocculant production and identify the correlation between the synthesis of polysaccharides and γ-PGA in B. licheniformis, a few key genes were investigated to explore their influence on the synthesis of the bioflocculants.
28950882	3	63	theme	bioflocculants	667:680	arg1	production					638:647	the production	634:647	the production of polysaccharide bioflocculants	634:680	Several key genes are involved in the metabolic pathway of polysaccharides in B. licheniformis, but the impacts of the regulation of these genes on the production of polysaccharide bioflocculants have not been illustrated completely.
28950882	7	64	theme	γ-PGA	1574:1578	arg1	synthesis					1537:1545	the synthesis	1533:1545	the synthesis of both polysaccharide and γ-PGA	1533:1578	In combination with an analysis of the transcriptional levels of several key genes involved in γ-PGA synthesis in B. licheniformis, we inferred that epsB played a key role in the synthesis of both polysaccharide and γ-PGA.
28950882	0	65	from	synthesis	97:105	arg1	licheniformis					147:159	Bacillus licheniformis	138:159	Bacillus licheniformis	138:159	Increasing the bioflocculant production and identifying the effect of overexpressing epsB on the synthesis of polysaccharide and γ-PGA in Bacillus licheniformis.
28950882	6	66	theme	44.03	1244:1248	arg1	%					1249:1249	%	1249:1249	%	1249:1249	The composition of the bioflocculant from the epsB recombinant strain was 28.95% total sugar, 3.464% protein and 44.03% γ-PGA, while in the original strain, these components represented 53.67%, 3.246% and 34.13%, respectively.
28950882	9	67	theme	phosphoglucomutase	1956:1973	arg1	expression					1942:1951	the tandem expression	1931:1951	the tandem expression of phosphoglucomutase (pgcA) and UTP-glucose-1-phosphate uridylyltransferase (gtaB1)	1931:2036	Moreover, we found that the tandem expression of phosphoglucomutase (pgcA) and UTP-glucose-1-phosphate uridylyltransferase (gtaB1) could enhance the crude yield of the bioflocculant by 20.77% and that the overexpression of epsA could enhance the bioflocculant yield by 23.70% compared with the original strain.
28950882	0	68	theme	overexpressing	70:83	arg1	epsB					85:88	overexpressing epsB	70:88	overexpressing epsB	70:88	Increasing the bioflocculant production and identifying the effect of overexpressing epsB on the synthesis of polysaccharide and γ-PGA in Bacillus licheniformis.
28950882	7	69	theme	polysaccharide	1555:1568	arg1	synthesis					1537:1545	the synthesis	1533:1545	the synthesis of both polysaccharide and γ-PGA	1533:1578	In combination with an analysis of the transcriptional levels of several key genes involved in γ-PGA synthesis in B. licheniformis, we inferred that epsB played a key role in the synthesis of both polysaccharide and γ-PGA.
28950882	4	70	theme	bioflocculant	736:748	arg1	production					750:759	the bioflocculant production	732:759	the bioflocculant production	732:759	To increase the bioflocculant production and identify the correlation between the synthesis of polysaccharides and γ-PGA in B. licheniformis, a few key genes were investigated to explore their influence on the synthesis of the bioflocculants.
28950882	1	71	theme	BACKGROUND	162:171	arg1	acid					209:212	poly-γ-glutamic acid	193:212	poly-γ-glutamic acid (γ-PGA)	193:220	BACKGROUND Polysaccharides and poly-γ-glutamic acid (γ-PGA) are biomacromolecules that have been reported as bioflocculants, and they exhibit high flocculating activity in many industrial applications.
28950882	1	71	theme	BACKGROUND	162:171	arg1	biomacromolecules					226:242	biomacromolecules	226:242	biomacromolecules that have been reported as bioflocculants	226:284	BACKGROUND Polysaccharides and poly-γ-glutamic acid (γ-PGA) are biomacromolecules that have been reported as bioflocculants, and they exhibit high flocculating activity in many industrial applications.
28950882	1	71	theme	BACKGROUND	162:171	arg1	Polysaccharides					173:187	BACKGROUND Polysaccharides	162:187	BACKGROUND Polysaccharides	162:187	BACKGROUND Polysaccharides and poly-γ-glutamic acid (γ-PGA) are biomacromolecules that have been reported as bioflocculants, and they exhibit high flocculating activity in many industrial applications.
28950882	3	72	from	impacts	590:596	arg1	production					638:647	the production	634:647	the production of polysaccharide bioflocculants	634:680	Several key genes are involved in the metabolic pathway of polysaccharides in B. licheniformis, but the impacts of the regulation of these genes on the production of polysaccharide bioflocculants have not been illustrated completely.
28950882	0	73	from	effect	60:65	arg1	synthesis					97:105	the synthesis	93:105	the synthesis of polysaccharide and γ-PGA in Bacillus licheniformis	93:159	Increasing the bioflocculant production and identifying the effect of overexpressing epsB on the synthesis of polysaccharide and γ-PGA in Bacillus licheniformis.
28950882	9	74	theme	uridylyltransferase	2010:2028	arg1	expression					1942:1951	the tandem expression	1931:1951	the tandem expression of phosphoglucomutase (pgcA) and UTP-glucose-1-phosphate uridylyltransferase (gtaB1)	1931:2036	Moreover, we found that the tandem expression of phosphoglucomutase (pgcA) and UTP-glucose-1-phosphate uridylyltransferase (gtaB1) could enhance the crude yield of the bioflocculant by 20.77% and that the overexpression of epsA could enhance the bioflocculant yield by 23.70% compared with the original strain.
28950882	0	75	theme	polysaccharide	110:123	arg1	synthesis					97:105	the synthesis	93:105	the synthesis of polysaccharide and γ-PGA in Bacillus licheniformis	93:159	Increasing the bioflocculant production and identifying the effect of overexpressing epsB on the synthesis of polysaccharide and γ-PGA in Bacillus licheniformis.
28950882	4	76	from	influence	913:921	arg1	synthesis					930:938	the synthesis	926:938	the synthesis of the bioflocculants	926:960	To increase the bioflocculant production and identify the correlation between the synthesis of polysaccharides and γ-PGA in B. licheniformis, a few key genes were investigated to explore their influence on the synthesis of the bioflocculants.
28950882	5	77	theme	bioflocculant	1039:1051	arg1	yield					1059:1063	the bioflocculant crude yield	1035:1063	the bioflocculant crude yield	1035:1063	RESULTS Overexpressing epsB from the eps gene cluster not only improved the bioflocculant crude yield by 13.98% but also enhanced the flocculating activity by 117.92%.
28950882	2	78	theme	γ-PGA	429:433	arg1	bioflocculants					435:448	γ-PGA bioflocculants	429:448	γ-PGA bioflocculants	429:448	Bacillus licheniformis CGMCC 2876 can produce polysaccharide and γ-PGA bioflocculants under different culture conditions.
28950882	5	79	theme	gene	1004:1007	arg1	cluster					1009:1015	the eps gene cluster	996:1015	the eps gene cluster	996:1015	RESULTS Overexpressing epsB from the eps gene cluster not only improved the bioflocculant crude yield by 13.98% but also enhanced the flocculating activity by 117.92%.
28950882	3	80	theme	Several	486:492	arg1	genes					498:502	Several key genes	486:502	Several key genes	486:502	Several key genes are involved in the metabolic pathway of polysaccharides in B. licheniformis, but the impacts of the regulation of these genes on the production of polysaccharide bioflocculants have not been illustrated completely.
28950882	8	81	theme	%	1844:1844	arg1	increases					1828:1836	increases	1828:1836	increases of 224% and 36.62%, respectively	1828:1869	The bioflocculant production of the epsB recombinant strain was further evaluated during batch fermentation in a 2 L fermenter; the flocculating activity reached 9612.75 U/mL, and the bioflocculant yield reached 10.26 g/L after 72 h, representing increases of 224% and 36.62%, respectively, compared with the original strain.
28950882	10	82	from	production	2301:2310	arg1	licheniformis					2318:2330	B. licheniformis	2315:2330	B. licheniformis	2315:2330	CONCLUSIONS This study provides a new method to greatly increase the bioflocculant production in B. licheniformis, and it demonstrates the correlation between the biosynthesis of polysaccharide and γ-PGA during EPS fermentation by regulating the expression of EpsB.
28950882	4	83	theme	polysaccharides	815:829	arg1	synthesis					802:810	the synthesis	798:810	the synthesis of polysaccharides	798:829	To increase the bioflocculant production and identify the correlation between the synthesis of polysaccharides and γ-PGA in B. licheniformis, a few key genes were investigated to explore their influence on the synthesis of the bioflocculants.
28950882	4	83	theme	polysaccharides	815:829	arg1	γ-PGA					835:839	γ-PGA	835:839	γ-PGA	835:839	To increase the bioflocculant production and identify the correlation between the synthesis of polysaccharides and γ-PGA in B. licheniformis, a few key genes were investigated to explore their influence on the synthesis of the bioflocculants.
28950882	2	84	theme	culture	466:472	arg1	conditions					474:483	different culture conditions	456:483	different culture conditions	456:483	Bacillus licheniformis CGMCC 2876 can produce polysaccharide and γ-PGA bioflocculants under different culture conditions.
28950882	1	85	theme	flocculating	309:320	arg1	activity					322:329	high flocculating activity	304:329	high flocculating activity in many industrial applications	304:361	BACKGROUND Polysaccharides and poly-γ-glutamic acid (γ-PGA) are biomacromolecules that have been reported as bioflocculants, and they exhibit high flocculating activity in many industrial applications.
28950882	7	86	theme	key	1521:1523	arg1	role					1525:1528	a key role	1519:1528	a key role	1519:1528	In combination with an analysis of the transcriptional levels of several key genes involved in γ-PGA synthesis in B. licheniformis, we inferred that epsB played a key role in the synthesis of both polysaccharide and γ-PGA.
28950882	6	87	from	strain	1194:1199	arg1	bioflocculant					1154:1166	the bioflocculant	1150:1166	the bioflocculant from the epsB recombinant strain	1150:1199	The composition of the bioflocculant from the epsB recombinant strain was 28.95% total sugar, 3.464% protein and 44.03% γ-PGA, while in the original strain, these components represented 53.67%, 3.246% and 34.13%, respectively.
28950882	6	87	from	strain	1194:1199	arg1	sugar					1218:1222	28.95% total sugar	1205:1222	28.95% total sugar	1205:1222	The composition of the bioflocculant from the epsB recombinant strain was 28.95% total sugar, 3.464% protein and 44.03% γ-PGA, while in the original strain, these components represented 53.67%, 3.246% and 34.13%, respectively.
28950882	6	87	from	strain	1194:1199	arg1	composition					1135:1145	The composition	1131:1145	The composition of the bioflocculant from the epsB recombinant strain	1131:1199	The composition of the bioflocculant from the epsB recombinant strain was 28.95% total sugar, 3.464% protein and 44.03% γ-PGA, while in the original strain, these components represented 53.67%, 3.246% and 34.13%, respectively.
28950882	3	88	theme	polysaccharides	545:559	arg1	pathway					534:540	the metabolic pathway	520:540	the metabolic pathway of polysaccharides in B. licheniformis	520:579	Several key genes are involved in the metabolic pathway of polysaccharides in B. licheniformis, but the impacts of the regulation of these genes on the production of polysaccharide bioflocculants have not been illustrated completely.
28950882	1	89	theme	industrial	339:348	arg1	applications					350:361	many industrial applications	334:361	many industrial applications	334:361	BACKGROUND Polysaccharides and poly-γ-glutamic acid (γ-PGA) are biomacromolecules that have been reported as bioflocculants, and they exhibit high flocculating activity in many industrial applications.
28950882	6	90	theme	epsB	1177:1180	arg1	strain					1194:1199	the epsB recombinant strain	1173:1199	the epsB recombinant strain	1173:1199	The composition of the bioflocculant from the epsB recombinant strain was 28.95% total sugar, 3.464% protein and 44.03% γ-PGA, while in the original strain, these components represented 53.67%, 3.246% and 34.13%, respectively.
28950882	8	91	theme	bioflocculant	1585:1597	arg1	production					1599:1608	The bioflocculant production	1581:1608	The bioflocculant production of the epsB recombinant strain	1581:1639	The bioflocculant production of the epsB recombinant strain was further evaluated during batch fermentation in a 2 L fermenter; the flocculating activity reached 9612.75 U/mL, and the bioflocculant yield reached 10.26 g/L after 72 h, representing increases of 224% and 36.62%, respectively, compared with the original strain.
28950882	4	92	theme	few	864:866	arg1	genes					872:876	a few key genes	862:876	a few key genes	862:876	To increase the bioflocculant production and identify the correlation between the synthesis of polysaccharides and γ-PGA in B. licheniformis, a few key genes were investigated to explore their influence on the synthesis of the bioflocculants.
27448235	3	0	with	polysaccharides	547:561	arg1	addition					572:579	the addition	568:579	the addition of structural glycoproteins, phenolic esters, minerals, lignin, and associated enzymes	568:666	The plant cell wall consists predominantly of polysaccharides with the addition of structural glycoproteins, phenolic esters, minerals, lignin, and associated enzymes.
27448235	6	1	theme	plant	1446:1450	arg1	development					1452:1462	plant development	1446:1462	plant development	1446:1462	These results demonstrate that the post-synthetic modification of cell wall components presents a valuable approach to investigate the potential signaling pathways induced during plant responses to such modifications that usually occur during plant development and stress responses.
27448235	2	2	theme	cell	385:388	arg1	walls					390:394	cell walls	385:394	cell walls	385:394	Besides mechanical function providing cell shape, cell walls participate in intercellular communication, defense during plant-microbe interactions, and plant growth.
27448235	4	3	theme	cell	847:850	arg1	integrity					857:865	cell wall integrity	847:865	cell wall integrity	847:865	Alterations in the cell wall composition created through either changes in biosynthesis of specific constituents or their post-synthetic modifications in the apoplast compromise cell wall integrity and frequently induce plant compensatory responses as a result of these alterations.
27448235	1	4	theme	cell	292:295	arg1	types					297:301	the different cell types	278:301	the different cell types	278:301	Cell walls are essential components of plant cells which perform a variety of important functions for the different cell types, tissues and organs of a plant.
27448235	5	5	theme	fucose	998:1003	arg1	residues					1005:1012	fucose residues	998:1012	fucose residues	998:1012	Here we report that post-synthetic removal of fucose residues specifically from arabinogalactan proteins in the Arabidopsis plant cell wall induces differential expression of fucosyltransferases and leads to the root and hypocotyl elongation changes.
27448235	4	6	theme	alterations	939:949	arg1	responses					908:916	plant compensatory responses	889:916	plant compensatory responses	889:916	Alterations in the cell wall composition created through either changes in biosynthesis of specific constituents or their post-synthetic modifications in the apoplast compromise cell wall integrity and frequently induce plant compensatory responses as a result of these alterations.
27448235	4	6	theme	alterations	939:949	arg1	result					923:928	a result	921:928	a result of these alterations	921:949	Alterations in the cell wall composition created through either changes in biosynthesis of specific constituents or their post-synthetic modifications in the apoplast compromise cell wall integrity and frequently induce plant compensatory responses as a result of these alterations.
27448235	5	7	theme	post-synthetic	972:985	arg1	removal					987:993	post-synthetic removal	972:993	post-synthetic removal of fucose residues specifically from arabinogalactan proteins in the Arabidopsis plant cell wall	972:1090	Here we report that post-synthetic removal of fucose residues specifically from arabinogalactan proteins in the Arabidopsis plant cell wall induces differential expression of fucosyltransferases and leads to the root and hypocotyl elongation changes.
27448235	5	8	theme	cell	1082:1085	arg1	wall					1087:1090	the Arabidopsis plant cell wall	1060:1090	the Arabidopsis plant cell wall	1060:1090	Here we report that post-synthetic removal of fucose residues specifically from arabinogalactan proteins in the Arabidopsis plant cell wall induces differential expression of fucosyltransferases and leads to the root and hypocotyl elongation changes.
27448235	0	9	theme	Compensatory	120:131	arg1	Upregulation					133:144	Compensatory Upregulation	120:144	Compensatory Upregulation of α-1,2-Fucosyltransferases	120:173	Post-Synthetic Defucosylation of AGP by Aspergillus nidulans α-1,2-Fucosidase Expressed in Arabidopsis Apoplast Induces Compensatory Upregulation of α-1,2-Fucosyltransferases.
27448235	6	10	theme	potential	1338:1346	arg1	pathways					1358:1365	the potential signaling pathways	1334:1365	the potential signaling pathways induced during plant responses to such modifications that usually occur during plant development and stress responses	1334:1483	These results demonstrate that the post-synthetic modification of cell wall components presents a valuable approach to investigate the potential signaling pathways induced during plant responses to such modifications that usually occur during plant development and stress responses.
27448235	5	11	from	removal	987:993	arg1	wall					1087:1090	the Arabidopsis plant cell wall	1060:1090	the Arabidopsis plant cell wall	1060:1090	Here we report that post-synthetic removal of fucose residues specifically from arabinogalactan proteins in the Arabidopsis plant cell wall induces differential expression of fucosyltransferases and leads to the root and hypocotyl elongation changes.
27448235	3	12	theme	glycoproteins	595:607	arg1	addition					572:579	the addition	568:579	the addition of structural glycoproteins, phenolic esters, minerals, lignin, and associated enzymes	568:666	The plant cell wall consists predominantly of polysaccharides with the addition of structural glycoproteins, phenolic esters, minerals, lignin, and associated enzymes.
27448235	4	13	from	changes	733:739	arg1	biosynthesis					744:755	biosynthesis	744:755	biosynthesis of specific constituents	744:780	Alterations in the cell wall composition created through either changes in biosynthesis of specific constituents or their post-synthetic modifications in the apoplast compromise cell wall integrity and frequently induce plant compensatory responses as a result of these alterations.
27448235	6	14	theme	components	1279:1288	arg1	modification					1253:1264	the post-synthetic modification	1234:1264	the post-synthetic modification of cell wall components	1234:1288	These results demonstrate that the post-synthetic modification of cell wall components presents a valuable approach to investigate the potential signaling pathways induced during plant responses to such modifications that usually occur during plant development and stress responses.
27448235	5	15	theme	Arabidopsis	1064:1074	arg1	wall					1087:1090	the Arabidopsis plant cell wall	1060:1090	the Arabidopsis plant cell wall	1060:1090	Here we report that post-synthetic removal of fucose residues specifically from arabinogalactan proteins in the Arabidopsis plant cell wall induces differential expression of fucosyltransferases and leads to the root and hypocotyl elongation changes.
27448235	5	16	theme	arabinogalactan	1032:1046	arg1	proteins					1048:1055	arabinogalactan proteins	1032:1055	arabinogalactan proteins	1032:1055	Here we report that post-synthetic removal of fucose residues specifically from arabinogalactan proteins in the Arabidopsis plant cell wall induces differential expression of fucosyltransferases and leads to the root and hypocotyl elongation changes.
27448235	3	17	theme	cell	511:514	arg1	wall					516:519	The plant cell wall	501:519	The plant cell wall	501:519	The plant cell wall consists predominantly of polysaccharides with the addition of structural glycoproteins, phenolic esters, minerals, lignin, and associated enzymes.
27448235	4	18	theme	wall	852:855	arg1	integrity					857:865	cell wall integrity	847:865	cell wall integrity	847:865	Alterations in the cell wall composition created through either changes in biosynthesis of specific constituents or their post-synthetic modifications in the apoplast compromise cell wall integrity and frequently induce plant compensatory responses as a result of these alterations.
27448235	1	19	theme	plant	215:219	arg1	cells					221:225	plant cells	215:225	plant cells	215:225	Cell walls are essential components of plant cells which perform a variety of important functions for the different cell types, tissues and organs of a plant.
27448235	5	20	attach	removal	987:993	arg3	wall					1087:1090	the Arabidopsis plant cell wall	1060:1090	the Arabidopsis plant cell wall	1060:1090	Here we report that post-synthetic removal of fucose residues specifically from arabinogalactan proteins in the Arabidopsis plant cell wall induces differential expression of fucosyltransferases and leads to the root and hypocotyl elongation changes.
27448235	5	20	attach	removal	987:993	arg2	residues					1005:1012	fucose residues	998:1012	fucose residues	998:1012	Here we report that post-synthetic removal of fucose residues specifically from arabinogalactan proteins in the Arabidopsis plant cell wall induces differential expression of fucosyltransferases and leads to the root and hypocotyl elongation changes.
27448235	6	21	theme	post-synthetic	1238:1251	arg1	modification					1253:1264	the post-synthetic modification	1234:1264	the post-synthetic modification of cell wall components	1234:1288	These results demonstrate that the post-synthetic modification of cell wall components presents a valuable approach to investigate the potential signaling pathways induced during plant responses to such modifications that usually occur during plant development and stress responses.
27448235	2	22	theme	plant	487:491	arg1	growth					493:498	plant growth	487:498	plant growth	487:498	Besides mechanical function providing cell shape, cell walls participate in intercellular communication, defense during plant-microbe interactions, and plant growth.
27448235	1	23	theme	cells	221:225	arg1	walls					181:185	Cell walls	176:185	Cell walls	176:185	Cell walls are essential components of plant cells which perform a variety of important functions for the different cell types, tissues and organs of a plant.
27448235	1	23	theme	cells	221:225	arg1	components					201:210	essential components	191:210	essential components of plant cells which perform a variety of important functions for the different cell types, tissues and organs of a plant	191:332	Cell walls are essential components of plant cells which perform a variety of important functions for the different cell types, tissues and organs of a plant.
27448235	5	24	theme	fucosyltransferases	1127:1145	arg1	expression					1113:1122	differential expression	1100:1122	differential expression of fucosyltransferases	1100:1145	Here we report that post-synthetic removal of fucose residues specifically from arabinogalactan proteins in the Arabidopsis plant cell wall induces differential expression of fucosyltransferases and leads to the root and hypocotyl elongation changes.
27448235	2	25	theme	mechanical	343:352	arg1	function					354:361	mechanical function	343:361	mechanical function	343:361	Besides mechanical function providing cell shape, cell walls participate in intercellular communication, defense during plant-microbe interactions, and plant growth.
27448235	2	26	theme	intercellular	411:423	arg1	communication					425:437	intercellular communication	411:437	intercellular communication	411:437	Besides mechanical function providing cell shape, cell walls participate in intercellular communication, defense during plant-microbe interactions, and plant growth.
27448235	0	27	theme	Post-Synthetic	0:13	arg1	Defucosylation					15:28	Post-Synthetic Defucosylation	0:28	Post-Synthetic Defucosylation of AGP by Aspergillus nidulans α-1,2-Fucosidase Expressed in Arabidopsis Apoplast	0:110	Post-Synthetic Defucosylation of AGP by Aspergillus nidulans α-1,2-Fucosidase Expressed in Arabidopsis Apoplast Induces Compensatory Upregulation of α-1,2-Fucosyltransferases.
27448235	4	28	from	modifications	806:818	arg1	biosynthesis					744:755	biosynthesis	744:755	biosynthesis of specific constituents	744:780	Alterations in the cell wall composition created through either changes in biosynthesis of specific constituents or their post-synthetic modifications in the apoplast compromise cell wall integrity and frequently induce plant compensatory responses as a result of these alterations.
27448235	3	29	theme	minerals	627:634	arg1	addition					572:579	the addition	568:579	the addition of structural glycoproteins, phenolic esters, minerals, lignin, and associated enzymes	568:666	The plant cell wall consists predominantly of polysaccharides with the addition of structural glycoproteins, phenolic esters, minerals, lignin, and associated enzymes.
27448235	0	30	theme	AGP	33:35	arg1	Defucosylation					15:28	Post-Synthetic Defucosylation	0:28	Post-Synthetic Defucosylation of AGP by Aspergillus nidulans α-1,2-Fucosidase Expressed in Arabidopsis Apoplast	0:110	Post-Synthetic Defucosylation of AGP by Aspergillus nidulans α-1,2-Fucosidase Expressed in Arabidopsis Apoplast Induces Compensatory Upregulation of α-1,2-Fucosyltransferases.
27448235	5	31	theme	differential	1100:1111	arg1	expression					1113:1122	differential expression	1100:1122	differential expression of fucosyltransferases	1100:1145	Here we report that post-synthetic removal of fucose residues specifically from arabinogalactan proteins in the Arabidopsis plant cell wall induces differential expression of fucosyltransferases and leads to the root and hypocotyl elongation changes.
27448235	3	32	theme	phenolic	610:617	arg1	esters					619:624	phenolic esters	610:624	phenolic esters	610:624	The plant cell wall consists predominantly of polysaccharides with the addition of structural glycoproteins, phenolic esters, minerals, lignin, and associated enzymes.
27448235	6	33	theme	plant	1382:1386	arg1	responses					1388:1396	plant responses	1382:1396	plant responses to such modifications that usually occur during plant development and stress responses	1382:1483	These results demonstrate that the post-synthetic modification of cell wall components presents a valuable approach to investigate the potential signaling pathways induced during plant responses to such modifications that usually occur during plant development and stress responses.
27448235	3	34	theme	plant	505:509	arg1	wall					516:519	The plant cell wall	501:519	The plant cell wall	501:519	The plant cell wall consists predominantly of polysaccharides with the addition of structural glycoproteins, phenolic esters, minerals, lignin, and associated enzymes.
27448235	4	35	theme	post-synthetic	791:804	arg1	modifications					806:818	their post-synthetic modifications	785:818	their post-synthetic modifications	785:818	Alterations in the cell wall composition created through either changes in biosynthesis of specific constituents or their post-synthetic modifications in the apoplast compromise cell wall integrity and frequently induce plant compensatory responses as a result of these alterations.
27448235	0	36	theme	α-1,2-Fucosyltransferases	149:173	arg1	Upregulation					133:144	Compensatory Upregulation	120:144	Compensatory Upregulation of α-1,2-Fucosyltransferases	120:173	Post-Synthetic Defucosylation of AGP by Aspergillus nidulans α-1,2-Fucosidase Expressed in Arabidopsis Apoplast Induces Compensatory Upregulation of α-1,2-Fucosyltransferases.
27448235	5	37	theme	hypocotyl	1173:1181	arg1	changes					1194:1200	the root and hypocotyl elongation changes	1160:1200	changes	1194:1200	Here we report that post-synthetic removal of fucose residues specifically from arabinogalactan proteins in the Arabidopsis plant cell wall induces differential expression of fucosyltransferases and leads to the root and hypocotyl elongation changes.
27448235	3	38	theme	esters	619:624	arg1	addition					572:579	the addition	568:579	the addition of structural glycoproteins, phenolic esters, minerals, lignin, and associated enzymes	568:666	The plant cell wall consists predominantly of polysaccharides with the addition of structural glycoproteins, phenolic esters, minerals, lignin, and associated enzymes.
27448235	2	39	theme	plant-microbe	455:467	arg1	interactions					469:480	plant-microbe interactions	455:480	plant-microbe interactions	455:480	Besides mechanical function providing cell shape, cell walls participate in intercellular communication, defense during plant-microbe interactions, and plant growth.
27448235	0	40	theme	Aspergillus	40:50	arg1	nidulans					52:59	Aspergillus nidulans	40:59	Aspergillus nidulans α-1,2-Fucosidase Expressed in Arabidopsis Apoplast	40:110	Post-Synthetic Defucosylation of AGP by Aspergillus nidulans α-1,2-Fucosidase Expressed in Arabidopsis Apoplast Induces Compensatory Upregulation of α-1,2-Fucosyltransferases.
27448235	4	41	theme	specific	760:767	arg1	constituents					769:780	specific constituents	760:780	specific constituents	760:780	Alterations in the cell wall composition created through either changes in biosynthesis of specific constituents or their post-synthetic modifications in the apoplast compromise cell wall integrity and frequently induce plant compensatory responses as a result of these alterations.
27448235	5	42	theme	elongation	1183:1192	arg1	changes					1194:1200	the root and hypocotyl elongation changes	1160:1200	changes	1194:1200	Here we report that post-synthetic removal of fucose residues specifically from arabinogalactan proteins in the Arabidopsis plant cell wall induces differential expression of fucosyltransferases and leads to the root and hypocotyl elongation changes.
27448235	4	43	theme	compensatory	895:906	arg1	responses					908:916	plant compensatory responses	889:916	plant compensatory responses	889:916	Alterations in the cell wall composition created through either changes in biosynthesis of specific constituents or their post-synthetic modifications in the apoplast compromise cell wall integrity and frequently induce plant compensatory responses as a result of these alterations.
27448235	4	43	theme	compensatory	895:906	arg1	result					923:928	a result	921:928	a result of these alterations	921:949	Alterations in the cell wall composition created through either changes in biosynthesis of specific constituents or their post-synthetic modifications in the apoplast compromise cell wall integrity and frequently induce plant compensatory responses as a result of these alterations.
27448235	4	44	theme	wall	693:696	arg1	composition					698:708	the cell wall composition	684:708	the cell wall composition created through either changes in biosynthesis of specific constituents or their post-synthetic modifications in the apoplast	684:834	Alterations in the cell wall composition created through either changes in biosynthesis of specific constituents or their post-synthetic modifications in the apoplast compromise cell wall integrity and frequently induce plant compensatory responses as a result of these alterations.
27448235	6	45	theme	such	1401:1404	arg1	modifications					1406:1418	such modifications	1401:1418	such modifications that usually occur during plant development and stress responses	1401:1483	These results demonstrate that the post-synthetic modification of cell wall components presents a valuable approach to investigate the potential signaling pathways induced during plant responses to such modifications that usually occur during plant development and stress responses.
27448235	1	46	theme	plant	328:332	arg1	types					297:301	the different cell types	278:301	the different cell types	278:301	Cell walls are essential components of plant cells which perform a variety of important functions for the different cell types, tissues and organs of a plant.
27448235	1	46	theme	plant	328:332	arg1	organs					316:321	organs	316:321	organs	316:321	Cell walls are essential components of plant cells which perform a variety of important functions for the different cell types, tissues and organs of a plant.
27448235	1	46	theme	plant	328:332	arg1	tissues					304:310	tissues	304:310	tissues	304:310	Cell walls are essential components of plant cells which perform a variety of important functions for the different cell types, tissues and organs of a plant.
27448235	0	47	gly	Defucosylation	15:28	arg1	AGP					33:35	AGP	33:35	AGP	33:35	Post-Synthetic Defucosylation of AGP by Aspergillus nidulans α-1,2-Fucosidase Expressed in Arabidopsis Apoplast Induces Compensatory Upregulation of α-1,2-Fucosyltransferases.
27448235	1	48	theme	important	254:262	arg1	functions					264:272	important functions	254:272	important functions for the different cell types, tissues and organs of a plant	254:332	Cell walls are essential components of plant cells which perform a variety of important functions for the different cell types, tissues and organs of a plant.
27448235	6	49	theme	stress	1468:1473	arg1	responses					1475:1483	stress responses	1468:1483	stress responses	1468:1483	These results demonstrate that the post-synthetic modification of cell wall components presents a valuable approach to investigate the potential signaling pathways induced during plant responses to such modifications that usually occur during plant development and stress responses.
27448235	4	50	theme	constituents	769:780	arg1	biosynthesis					744:755	biosynthesis	744:755	biosynthesis of specific constituents	744:780	Alterations in the cell wall composition created through either changes in biosynthesis of specific constituents or their post-synthetic modifications in the apoplast compromise cell wall integrity and frequently induce plant compensatory responses as a result of these alterations.
27448235	3	51	theme	lignin	637:642	arg1	addition					572:579	the addition	568:579	the addition of structural glycoproteins, phenolic esters, minerals, lignin, and associated enzymes	568:666	The plant cell wall consists predominantly of polysaccharides with the addition of structural glycoproteins, phenolic esters, minerals, lignin, and associated enzymes.
27448235	2	52	theme	cell	373:376	arg1	shape					378:382	cell shape	373:382	cell shape	373:382	Besides mechanical function providing cell shape, cell walls participate in intercellular communication, defense during plant-microbe interactions, and plant growth.
27448235	5	53	from	proteins	1048:1055	arg1	removal					987:993	post-synthetic removal	972:993	post-synthetic removal of fucose residues specifically from arabinogalactan proteins in the Arabidopsis plant cell wall	972:1090	Here we report that post-synthetic removal of fucose residues specifically from arabinogalactan proteins in the Arabidopsis plant cell wall induces differential expression of fucosyltransferases and leads to the root and hypocotyl elongation changes.
27448235	6	54	theme	signaling	1348:1356	arg1	pathways					1358:1365	the potential signaling pathways	1334:1365	the potential signaling pathways induced during plant responses to such modifications that usually occur during plant development and stress responses	1334:1483	These results demonstrate that the post-synthetic modification of cell wall components presents a valuable approach to investigate the potential signaling pathways induced during plant responses to such modifications that usually occur during plant development and stress responses.
27448235	4	55	theme	plant	889:893	arg1	responses					908:916	plant compensatory responses	889:916	plant compensatory responses	889:916	Alterations in the cell wall composition created through either changes in biosynthesis of specific constituents or their post-synthetic modifications in the apoplast compromise cell wall integrity and frequently induce plant compensatory responses as a result of these alterations.
27448235	4	55	theme	plant	889:893	arg1	result					923:928	a result	921:928	a result of these alterations	921:949	Alterations in the cell wall composition created through either changes in biosynthesis of specific constituents or their post-synthetic modifications in the apoplast compromise cell wall integrity and frequently induce plant compensatory responses as a result of these alterations.
27448235	1	56	theme	Cell	176:179	arg1	walls					181:185	Cell walls	176:185	Cell walls	176:185	Cell walls are essential components of plant cells which perform a variety of important functions for the different cell types, tissues and organs of a plant.
27448235	1	56	theme	Cell	176:179	arg1	components					201:210	essential components	191:210	essential components of plant cells which perform a variety of important functions for the different cell types, tissues and organs of a plant	191:332	Cell walls are essential components of plant cells which perform a variety of important functions for the different cell types, tissues and organs of a plant.
27448235	6	57	theme	wall	1274:1277	arg1	components					1279:1288	cell wall components	1269:1288	cell wall components	1269:1288	These results demonstrate that the post-synthetic modification of cell wall components presents a valuable approach to investigate the potential signaling pathways induced during plant responses to such modifications that usually occur during plant development and stress responses.
27448235	1	58	theme	functions	264:272	arg1	variety					243:249	a variety	241:249	a variety of important functions for the different cell types, tissues and organs of a plant	241:332	Cell walls are essential components of plant cells which perform a variety of important functions for the different cell types, tissues and organs of a plant.
27448235	1	58	theme	functions	264:272	arg1	functions					264:272	important functions	254:272	important functions for the different cell types, tissues and organs of a plant	254:332	Cell walls are essential components of plant cells which perform a variety of important functions for the different cell types, tissues and organs of a plant.
27448235	3	59	theme	structural	584:593	arg1	glycoproteins					595:607	structural glycoproteins	584:607	structural glycoproteins	584:607	The plant cell wall consists predominantly of polysaccharides with the addition of structural glycoproteins, phenolic esters, minerals, lignin, and associated enzymes.
27448235	5	60	theme	plant	1076:1080	arg1	wall					1087:1090	the Arabidopsis plant cell wall	1060:1090	the Arabidopsis plant cell wall	1060:1090	Here we report that post-synthetic removal of fucose residues specifically from arabinogalactan proteins in the Arabidopsis plant cell wall induces differential expression of fucosyltransferases and leads to the root and hypocotyl elongation changes.
27448235	1	61	theme	essential	191:199	arg1	walls					181:185	Cell walls	176:185	Cell walls	176:185	Cell walls are essential components of plant cells which perform a variety of important functions for the different cell types, tissues and organs of a plant.
27448235	1	61	theme	essential	191:199	arg1	components					201:210	essential components	191:210	essential components of plant cells which perform a variety of important functions for the different cell types, tissues and organs of a plant	191:332	Cell walls are essential components of plant cells which perform a variety of important functions for the different cell types, tissues and organs of a plant.
27448235	5	62	theme	residues	1005:1012	arg1	removal					987:993	post-synthetic removal	972:993	post-synthetic removal of fucose residues specifically from arabinogalactan proteins in the Arabidopsis plant cell wall	972:1090	Here we report that post-synthetic removal of fucose residues specifically from arabinogalactan proteins in the Arabidopsis plant cell wall induces differential expression of fucosyltransferases and leads to the root and hypocotyl elongation changes.
27448235	4	63	from	Alterations	669:679	arg1	composition					698:708	the cell wall composition	684:708	the cell wall composition created through either changes in biosynthesis of specific constituents or their post-synthetic modifications in the apoplast	684:834	Alterations in the cell wall composition created through either changes in biosynthesis of specific constituents or their post-synthetic modifications in the apoplast compromise cell wall integrity and frequently induce plant compensatory responses as a result of these alterations.
27448235	4	64	theme	cell	688:691	arg1	composition					698:708	the cell wall composition	684:708	the cell wall composition created through either changes in biosynthesis of specific constituents or their post-synthetic modifications in the apoplast	684:834	Alterations in the cell wall composition created through either changes in biosynthesis of specific constituents or their post-synthetic modifications in the apoplast compromise cell wall integrity and frequently induce plant compensatory responses as a result of these alterations.
27448235	3	65	theme	associated	649:658	arg1	enzymes					660:666	associated enzymes	649:666	associated enzymes	649:666	The plant cell wall consists predominantly of polysaccharides with the addition of structural glycoproteins, phenolic esters, minerals, lignin, and associated enzymes.
27448235	6	66	theme	valuable	1301:1308	arg1	approach					1310:1317	a valuable approach	1299:1317	a valuable approach to investigate the potential signaling pathways induced during plant responses to such modifications that usually occur during plant development and stress responses	1299:1483	These results demonstrate that the post-synthetic modification of cell wall components presents a valuable approach to investigate the potential signaling pathways induced during plant responses to such modifications that usually occur during plant development and stress responses.
27448235	6	67	theme	cell	1269:1272	arg1	components					1279:1288	cell wall components	1269:1288	cell wall components	1269:1288	These results demonstrate that the post-synthetic modification of cell wall components presents a valuable approach to investigate the potential signaling pathways induced during plant responses to such modifications that usually occur during plant development and stress responses.
27448235	3	68	theme	enzymes	660:666	arg1	addition					572:579	the addition	568:579	the addition of structural glycoproteins, phenolic esters, minerals, lignin, and associated enzymes	568:666	The plant cell wall consists predominantly of polysaccharides with the addition of structural glycoproteins, phenolic esters, minerals, lignin, and associated enzymes.
27448235	3	69	gly	glycoproteins	595:607	arg1	glycoproteins					595:607	structural glycoproteins	584:607	structural glycoproteins	584:607	The plant cell wall consists predominantly of polysaccharides with the addition of structural glycoproteins, phenolic esters, minerals, lignin, and associated enzymes.
27448235	1	70	theme	different	282:290	arg1	types					297:301	the different cell types	278:301	the different cell types	278:301	Cell walls are essential components of plant cells which perform a variety of important functions for the different cell types, tissues and organs of a plant.
29120180	4	0	theme	antibodies	662:671	arg1	mapping					636:642	the detailed epitope mapping	615:642	the detailed epitope mapping of two monoclonal antibodies known to recognize AGPs	615:695	Carbohydrate microarrays were generated to enable the detailed epitope mapping of two monoclonal antibodies known to recognize AGPs: JIM16 and JIM133.
29120180	2	1	theme	thioglycoside	293:305	arg1	donors					307:312	thioglycoside donors	293:312	thioglycoside donors	293:312	The synthetic strategy relies on iterative couplings of monosaccharide and disaccharide thioglycoside donors, followed by a late-stage glycosylation of heptagalactan backbone acceptors to introduce branching.
29120180	2	2	dep	monosaccharide	261:274	arg1	donors					307:312	thioglycoside donors	293:312	thioglycoside donors	293:312	The synthetic strategy relies on iterative couplings of monosaccharide and disaccharide thioglycoside donors, followed by a late-stage glycosylation of heptagalactan backbone acceptors to introduce branching.
29120180	2	3	theme	synthetic	209:217	arg1	strategy					219:226	The synthetic strategy	205:226	The synthetic strategy	205:226	The synthetic strategy relies on iterative couplings of monosaccharide and disaccharide thioglycoside donors, followed by a late-stage glycosylation of heptagalactan backbone acceptors to introduce branching.
29120180	3	4	theme	protective	475:484	arg1	groups					486:491	protective groups	475:491	protective groups in order to tune reactivity and ensure selectivity during the assembly	475:562	A key finding from the synthetic study was the need to match protective groups in order to tune reactivity and ensure selectivity during the assembly.
29120180	4	5	theme	detailed	619:626	arg1	mapping					636:642	the detailed epitope mapping	615:642	the detailed epitope mapping of two monoclonal antibodies known to recognize AGPs	615:695	Carbohydrate microarrays were generated to enable the detailed epitope mapping of two monoclonal antibodies known to recognize AGPs: JIM16 and JIM133.
29120180	2	6	gly	glycosylation	340:352	arg1	acceptors					380:388	heptagalactan backbone acceptors	357:388	heptagalactan backbone acceptors	357:388	The synthetic strategy relies on iterative couplings of monosaccharide and disaccharide thioglycoside donors, followed by a late-stage glycosylation of heptagalactan backbone acceptors to introduce branching.
29120180	2	7	theme	late-stage	329:338	arg1	glycosylation					340:352	a late-stage glycosylation	327:352	a late-stage glycosylation of heptagalactan backbone acceptors to introduce branching	327:411	The synthetic strategy relies on iterative couplings of monosaccharide and disaccharide thioglycoside donors, followed by a late-stage glycosylation of heptagalactan backbone acceptors to introduce branching.
29120180	1	8	theme	linear	81:86	arg1	-d-galactans					118:129	linear and (1 → 6)-branched β-(1 → 3)-d-galactans	81:129	linear and (1 → 6)-branched β-(1 → 3)-d-galactans	81:129	The synthesis of linear and (1 → 6)-branched β-(1 → 3)-d-galactans, structures found in plant arabinogalactan proteins (AGPs), is described.
29120180	1	8	theme	linear	81:86	arg1	structures					132:141	structures	132:141	structures found in plant arabinogalactan proteins (AGPs)	132:188	The synthesis of linear and (1 → 6)-branched β-(1 → 3)-d-galactans, structures found in plant arabinogalactan proteins (AGPs), is described.
29120180	1	8	theme	linear	81:86	arg1	→					114:114	1 → 3	112:116	1 → 3	112:116	The synthesis of linear and (1 → 6)-branched β-(1 → 3)-d-galactans, structures found in plant arabinogalactan proteins (AGPs), is described.
29120180	1	9	theme	-d-galactans	118:129	arg1	synthesis					68:76	The synthesis	64:76	The synthesis of linear and (1 → 6)-branched β-(1 → 3)-d-galactans, structures found in plant arabinogalactan proteins (AGPs),	64:189	The synthesis of linear and (1 → 6)-branched β-(1 → 3)-d-galactans, structures found in plant arabinogalactan proteins (AGPs), is described.
29120180	3	10	from	study	447:451	arg1	need					461:464	the need to match protective groups in order to tune reactivity and ensure selectivity during the assembly	457:562	the need to match protective groups in order to tune reactivity and ensure selectivity during the assembly	457:562	A key finding from the synthetic study was the need to match protective groups in order to tune reactivity and ensure selectivity during the assembly.
29120180	3	10	from	study	447:451	arg1	finding					420:426	A key finding	414:426	A key finding from the synthetic study	414:451	A key finding from the synthetic study was the need to match protective groups in order to tune reactivity and ensure selectivity during the assembly.
29120180	2	11	theme	iterative	238:246	arg1	couplings					248:256	iterative couplings	238:256	iterative couplings of monosaccharide and disaccharide thioglycoside donors	238:312	The synthetic strategy relies on iterative couplings of monosaccharide and disaccharide thioglycoside donors, followed by a late-stage glycosylation of heptagalactan backbone acceptors to introduce branching.
29120180	4	12	theme	epitope	628:634	arg1	mapping					636:642	the detailed epitope mapping	615:642	the detailed epitope mapping of two monoclonal antibodies known to recognize AGPs	615:695	Carbohydrate microarrays were generated to enable the detailed epitope mapping of two monoclonal antibodies known to recognize AGPs: JIM16 and JIM133.
29120180	1	13	dep	β-	109:110	arg1	→					95:95	1 → 6	93:97	1 → 6	93:97	The synthesis of linear and (1 → 6)-branched β-(1 → 3)-d-galactans, structures found in plant arabinogalactan proteins (AGPs), is described.
29120180	2	14	theme	acceptors	380:388	arg1	glycosylation					340:352	a late-stage glycosylation	327:352	a late-stage glycosylation of heptagalactan backbone acceptors to introduce branching	327:411	The synthetic strategy relies on iterative couplings of monosaccharide and disaccharide thioglycoside donors, followed by a late-stage glycosylation of heptagalactan backbone acceptors to introduce branching.
29120180	4	15	theme	monoclonal	651:660	arg1	antibodies					662:671	two monoclonal antibodies	647:671	two monoclonal antibodies known to recognize AGPs	647:695	Carbohydrate microarrays were generated to enable the detailed epitope mapping of two monoclonal antibodies known to recognize AGPs: JIM16 and JIM133.
29120180	2	16	theme	backbone	371:378	arg1	acceptors					380:388	heptagalactan backbone acceptors	357:388	heptagalactan backbone acceptors	357:388	The synthetic strategy relies on iterative couplings of monosaccharide and disaccharide thioglycoside donors, followed by a late-stage glycosylation of heptagalactan backbone acceptors to introduce branching.
29120180	0	17	theme	Type	38:41	arg1	Arabinogalactans					46:61	Branched Type II Arabinogalactans	29:61	Branched Type II Arabinogalactans	29:61	Synthesis and Application of Branched Type II Arabinogalactans.
29120180	2	18	theme	heptagalactan	357:369	arg1	acceptors					380:388	heptagalactan backbone acceptors	357:388	heptagalactan backbone acceptors	357:388	The synthetic strategy relies on iterative couplings of monosaccharide and disaccharide thioglycoside donors, followed by a late-stage glycosylation of heptagalactan backbone acceptors to introduce branching.
29120180	0	19	theme	Branched	29:36	arg1	Arabinogalactans					46:61	Branched Type II Arabinogalactans	29:61	Branched Type II Arabinogalactans	29:61	Synthesis and Application of Branched Type II Arabinogalactans.
29120180	1	20	theme	plant	152:156	arg1	AGPs					184:187	AGPs	184:187	AGPs	184:187	The synthesis of linear and (1 → 6)-branched β-(1 → 3)-d-galactans, structures found in plant arabinogalactan proteins (AGPs), is described.
29120180	1	20	theme	plant	152:156	arg1	proteins					174:181	plant arabinogalactan proteins	152:181	plant arabinogalactan proteins (AGPs)	152:188	The synthesis of linear and (1 → 6)-branched β-(1 → 3)-d-galactans, structures found in plant arabinogalactan proteins (AGPs), is described.
29120180	0	21	theme	Arabinogalactans	46:61	arg1	Application					14:24	Application	14:24	Application	14:24	Synthesis and Application of Branched Type II Arabinogalactans.
29120180	0	21	theme	Arabinogalactans	46:61	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis and Application of Branched Type II Arabinogalactans.
29120180	4	22	dep	generated	595:603	arg1	JIM133					708:713	JIM133	708:713	JIM133	708:713	Carbohydrate microarrays were generated to enable the detailed epitope mapping of two monoclonal antibodies known to recognize AGPs: JIM16 and JIM133.
29120180	4	22	dep	generated	595:603	arg1	JIM16					698:702	JIM16	698:702	JIM16	698:702	Carbohydrate microarrays were generated to enable the detailed epitope mapping of two monoclonal antibodies known to recognize AGPs: JIM16 and JIM133.
29120180	2	23	theme	disaccharide	280:291	arg1	couplings					248:256	iterative couplings	238:256	iterative couplings of monosaccharide and disaccharide thioglycoside donors	238:312	The synthetic strategy relies on iterative couplings of monosaccharide and disaccharide thioglycoside donors, followed by a late-stage glycosylation of heptagalactan backbone acceptors to introduce branching.
29120180	0	24	theme	II	43:44	arg1	Arabinogalactans					46:61	Branched Type II Arabinogalactans	29:61	Branched Type II Arabinogalactans	29:61	Synthesis and Application of Branched Type II Arabinogalactans.
29120180	1	25	located	found	143:147	arg1	AGPs					184:187	AGPs	184:187	AGPs	184:187	The synthesis of linear and (1 → 6)-branched β-(1 → 3)-d-galactans, structures found in plant arabinogalactan proteins (AGPs), is described.
29120180	1	25	located	found	143:147	arg2	-d-galactans					118:129	linear and (1 → 6)-branched β-(1 → 3)-d-galactans	81:129	linear and (1 → 6)-branched β-(1 → 3)-d-galactans	81:129	The synthesis of linear and (1 → 6)-branched β-(1 → 3)-d-galactans, structures found in plant arabinogalactan proteins (AGPs), is described.
29120180	1	25	located	found	143:147	arg1	proteins					174:181	plant arabinogalactan proteins	152:181	plant arabinogalactan proteins (AGPs)	152:188	The synthesis of linear and (1 → 6)-branched β-(1 → 3)-d-galactans, structures found in plant arabinogalactan proteins (AGPs), is described.
29120180	1	25	located	found	143:147	arg2	structures					132:141	structures	132:141	structures found in plant arabinogalactan proteins (AGPs)	132:188	The synthesis of linear and (1 → 6)-branched β-(1 → 3)-d-galactans, structures found in plant arabinogalactan proteins (AGPs), is described.
29120180	2	26	dep	relies	228:233	arg1	followed					315:322	followed	315:322	followed by a late-stage glycosylation of heptagalactan backbone acceptors to introduce branching	315:411	The synthetic strategy relies on iterative couplings of monosaccharide and disaccharide thioglycoside donors, followed by a late-stage glycosylation of heptagalactan backbone acceptors to introduce branching.
29120180	2	27	theme	monosaccharide	261:274	arg1	couplings					248:256	iterative couplings	238:256	iterative couplings of monosaccharide and disaccharide thioglycoside donors	238:312	The synthetic strategy relies on iterative couplings of monosaccharide and disaccharide thioglycoside donors, followed by a late-stage glycosylation of heptagalactan backbone acceptors to introduce branching.
29120180	1	28	theme	arabinogalactan	158:172	arg1	AGPs					184:187	AGPs	184:187	AGPs	184:187	The synthesis of linear and (1 → 6)-branched β-(1 → 3)-d-galactans, structures found in plant arabinogalactan proteins (AGPs), is described.
29120180	1	28	theme	arabinogalactan	158:172	arg1	proteins					174:181	plant arabinogalactan proteins	152:181	plant arabinogalactan proteins (AGPs)	152:188	The synthesis of linear and (1 → 6)-branched β-(1 → 3)-d-galactans, structures found in plant arabinogalactan proteins (AGPs), is described.
29120180	3	29	theme	synthetic	437:445	arg1	study					447:451	the synthetic study	433:451	the synthetic study	433:451	A key finding from the synthetic study was the need to match protective groups in order to tune reactivity and ensure selectivity during the assembly.
29120180	3	30	theme	key	416:418	arg1	need					461:464	the need to match protective groups in order to tune reactivity and ensure selectivity during the assembly	457:562	the need to match protective groups in order to tune reactivity and ensure selectivity during the assembly	457:562	A key finding from the synthetic study was the need to match protective groups in order to tune reactivity and ensure selectivity during the assembly.
29120180	3	30	theme	key	416:418	arg1	finding					420:426	A key finding	414:426	A key finding from the synthetic study	414:451	A key finding from the synthetic study was the need to match protective groups in order to tune reactivity and ensure selectivity during the assembly.
29120180	1	31	theme	β-	109:110	arg1	-d-galactans					118:129	linear and (1 → 6)-branched β-(1 → 3)-d-galactans	81:129	linear and (1 → 6)-branched β-(1 → 3)-d-galactans	81:129	The synthesis of linear and (1 → 6)-branched β-(1 → 3)-d-galactans, structures found in plant arabinogalactan proteins (AGPs), is described.
29120180	1	31	theme	β-	109:110	arg1	structures					132:141	structures	132:141	structures found in plant arabinogalactan proteins (AGPs)	132:188	The synthesis of linear and (1 → 6)-branched β-(1 → 3)-d-galactans, structures found in plant arabinogalactan proteins (AGPs), is described.
29120180	1	31	theme	β-	109:110	arg1	→					114:114	1 → 3	112:116	1 → 3	112:116	The synthesis of linear and (1 → 6)-branched β-(1 → 3)-d-galactans, structures found in plant arabinogalactan proteins (AGPs), is described.
29120180	4	32	theme	Carbohydrate	565:576	arg1	microarrays					578:588	Carbohydrate microarrays	565:588	Carbohydrate microarrays	565:588	Carbohydrate microarrays were generated to enable the detailed epitope mapping of two monoclonal antibodies known to recognize AGPs: JIM16 and JIM133.
25498203	5	0	from	H	797:797	arg1	strain					830:835	strain G3977	830:841	strain G3977	830:841	The following structures of the O-polysaccharides were established: [Formula: see text] where R indicates H in strain G3983 or α-D-Glcp in strain G3977, d-Fuc3NAlaAc indicates 3-(N-acetyl-L-alanyl)amino-3,6-dideoxy-D-galactose.
25498203	5	0	from	H	797:797	arg1	strain					802:807	strain G3983	802:813	strain G3983	802:813	The following structures of the O-polysaccharides were established: [Formula: see text] where R indicates H in strain G3983 or α-D-Glcp in strain G3977, d-Fuc3NAlaAc indicates 3-(N-acetyl-L-alanyl)amino-3,6-dideoxy-D-galactose.
25498203	2	1	theme	opportunistic	180:192	arg1	pathogens					200:208	opportunistic human pathogens	180:208	opportunistic human pathogens linked with life-threatening infections predominantly in neonates	180:274	are emerging opportunistic human pathogens linked with life-threatening infections predominantly in neonates.
25498203	6	2	theme	C.	996:997	arg1	Pathog					1025:1030	C. dublinensis O1 (Foodborne Pathog	996:1030	C. dublinensis O1 (Foodborne Pathog	996:1030	Both strains share the O-antigen gene cluster, which is identical to that of C. dublinensis O1 (Foodborne Pathog.
25498203	0	3	theme	G3977	81:85	arg1	O-polysaccharides					26:42	the O-polysaccharides	22:42	the O-polysaccharides of Cronobacter dublinensis G3983 and G3977	22:85	Related structures of the O-polysaccharides of Cronobacter dublinensis G3983 and G3977 containing 3-(N-acetyl-L-alanyl)amino-3,6-dideoxy-D-galactose.
25498203	2	4	from	infections	239:248	arg1	neonates					267:274	neonates	267:274	neonates	267:274	are emerging opportunistic human pathogens linked with life-threatening infections predominantly in neonates.
25498203	0	5	theme	3-	98:99	arg1	amino-3,6-dideoxy-D-galactose					119:147	3-(N-acetyl-L-alanyl)amino-3,6-dideoxy-D-galactose	98:147	3-(N-acetyl-L-alanyl)amino-3,6-dideoxy-D-galactose	98:147	Related structures of the O-polysaccharides of Cronobacter dublinensis G3983 and G3977 containing 3-(N-acetyl-L-alanyl)amino-3,6-dideoxy-D-galactose.
25498203	4	6	theme	alkaline	516:523	arg1	degradation					525:535	mild alkaline degradation	511:535	mild alkaline degradation of the lipopolysaccharides of C. dublinensis G3983 and G3977	511:596	In this work, short-chain O-polysaccharides consisting on the average of 2-3 repeating units were obtained by mild acid or mild alkaline degradation of the lipopolysaccharides of C. dublinensis G3983 and G3977 and studied by composition analysis, Smith degradation, and (1)H and (13)C NMR spectroscopy.
25498203	4	7	theme	2-3	461:463	arg1	units					475:479	2-3 repeating units	461:479	2-3 repeating units	461:479	In this work, short-chain O-polysaccharides consisting on the average of 2-3 repeating units were obtained by mild acid or mild alkaline degradation of the lipopolysaccharides of C. dublinensis G3983 and G3977 and studied by composition analysis, Smith degradation, and (1)H and (13)C NMR spectroscopy.
25498203	4	8	theme	Smith	635:639	arg1	degradation					641:651	Smith degradation	635:651	Smith degradation	635:651	In this work, short-chain O-polysaccharides consisting on the average of 2-3 repeating units were obtained by mild acid or mild alkaline degradation of the lipopolysaccharides of C. dublinensis G3983 and G3977 and studied by composition analysis, Smith degradation, and (1)H and (13)C NMR spectroscopy.
25498203	8	9	theme	G3983	1149:1153	arg1	structure					1121:1129	the O-antigen structure	1107:1129	the O-antigen structure of C. dublinensis G3983	1107:1153	The assigned gene functions are in agreement with the O-antigen structure of C. dublinensis G3983, and the side-chain glucosylation of the O-antigen of C. dublinensis G3977 is evidently encoded elsewhere in the genome.
25498203	4	10	theme	mild	511:514	arg1	degradation					525:535	mild alkaline degradation	511:535	mild alkaline degradation of the lipopolysaccharides of C. dublinensis G3983 and G3977	511:596	In this work, short-chain O-polysaccharides consisting on the average of 2-3 repeating units were obtained by mild acid or mild alkaline degradation of the lipopolysaccharides of C. dublinensis G3983 and G3977 and studied by composition analysis, Smith degradation, and (1)H and (13)C NMR spectroscopy.
25498203	8	11	theme	G3977	1224:1228	arg1	O-antigen					1196:1204	the O-antigen	1192:1204	the O-antigen of C. dublinensis G3977	1192:1228	The assigned gene functions are in agreement with the O-antigen structure of C. dublinensis G3983, and the side-chain glucosylation of the O-antigen of C. dublinensis G3977 is evidently encoded elsewhere in the genome.
25498203	8	12	theme	C.	1209:1210	arg1	G3977					1224:1228	C. dublinensis G3977	1209:1228	C. dublinensis G3977	1209:1228	The assigned gene functions are in agreement with the O-antigen structure of C. dublinensis G3983, and the side-chain glucosylation of the O-antigen of C. dublinensis G3977 is evidently encoded elsewhere in the genome.
25498203	6	13	theme	O-antigen	942:950	arg1	cluster					957:963	the O-antigen gene cluster	938:963	the O-antigen gene cluster	938:963	Both strains share the O-antigen gene cluster, which is identical to that of C. dublinensis O1 (Foodborne Pathog.
25498203	6	13	theme	O-antigen	942:950	arg1	identical					975:983	identical	975:983	identical	975:983	Both strains share the O-antigen gene cluster, which is identical to that of C. dublinensis O1 (Foodborne Pathog.
25498203	3	14	theme	niche	370:374	arg1	adaptation					376:385	niche adaptation	370:385	niche adaptation	370:385	O-Antigen (O-polysaccharide) is highly variable and plays an important role in virulence and niche adaptation.
25498203	4	15	theme	short-chain	402:412	arg1	O-polysaccharides					414:430	short-chain O-polysaccharides	402:430	short-chain O-polysaccharides consisting on the average of 2-3 repeating units	402:479	In this work, short-chain O-polysaccharides consisting on the average of 2-3 repeating units were obtained by mild acid or mild alkaline degradation of the lipopolysaccharides of C. dublinensis G3983 and G3977 and studied by composition analysis, Smith degradation, and (1)H and (13)C NMR spectroscopy.
25498203	8	16	theme	dublinensis	1212:1222	arg1	G3977					1224:1228	C. dublinensis G3977	1209:1228	C. dublinensis G3977	1209:1228	The assigned gene functions are in agreement with the O-antigen structure of C. dublinensis G3983, and the side-chain glucosylation of the O-antigen of C. dublinensis G3977 is evidently encoded elsewhere in the genome.
25498203	4	17	theme	NMR	673:675	arg1	spectroscopy					677:688	(13)C NMR spectroscopy	667:688	(13)C NMR spectroscopy	667:688	In this work, short-chain O-polysaccharides consisting on the average of 2-3 repeating units were obtained by mild acid or mild alkaline degradation of the lipopolysaccharides of C. dublinensis G3983 and G3977 and studied by composition analysis, Smith degradation, and (1)H and (13)C NMR spectroscopy.
25498203	0	18	theme	Related	0:6	arg1	structures					8:17	Related structures	0:17	Related structures of the O-polysaccharides of Cronobacter dublinensis G3983 and G3977	0:85	Related structures of the O-polysaccharides of Cronobacter dublinensis G3983 and G3977 containing 3-(N-acetyl-L-alanyl)amino-3,6-dideoxy-D-galactose.
25498203	4	19	theme	C	671:671	arg1	spectroscopy					677:688	(13)C NMR spectroscopy	667:688	(13)C NMR spectroscopy	667:688	In this work, short-chain O-polysaccharides consisting on the average of 2-3 repeating units were obtained by mild acid or mild alkaline degradation of the lipopolysaccharides of C. dublinensis G3983 and G3977 and studied by composition analysis, Smith degradation, and (1)H and (13)C NMR spectroscopy.
25498203	8	20	theme	side-chain	1164:1173	arg1	glucosylation					1175:1187	the side-chain glucosylation	1160:1187	the side-chain glucosylation of the O-antigen of C. dublinensis G3977	1160:1228	The assigned gene functions are in agreement with the O-antigen structure of C. dublinensis G3983, and the side-chain glucosylation of the O-antigen of C. dublinensis G3977 is evidently encoded elsewhere in the genome.
25498203	4	21	theme	composition	613:623	arg1	analysis					625:632	composition analysis	613:632	composition analysis	613:632	In this work, short-chain O-polysaccharides consisting on the average of 2-3 repeating units were obtained by mild acid or mild alkaline degradation of the lipopolysaccharides of C. dublinensis G3983 and G3977 and studied by composition analysis, Smith degradation, and (1)H and (13)C NMR spectroscopy.
25498203	4	22	theme	mild	498:501	arg1	acid					503:506	mild acid	498:506	mild acid	498:506	In this work, short-chain O-polysaccharides consisting on the average of 2-3 repeating units were obtained by mild acid or mild alkaline degradation of the lipopolysaccharides of C. dublinensis G3983 and G3977 and studied by composition analysis, Smith degradation, and (1)H and (13)C NMR spectroscopy.
25498203	8	23	theme	gene	1070:1073	arg1	functions					1075:1083	The assigned gene functions	1057:1083	The assigned gene functions	1057:1083	The assigned gene functions are in agreement with the O-antigen structure of C. dublinensis G3983, and the side-chain glucosylation of the O-antigen of C. dublinensis G3977 is evidently encoded elsewhere in the genome.
25498203	5	24	theme	3-	867:868	arg1	amino-3,6-dideoxy-D-galactose					888:916	3-(N-acetyl-L-alanyl)amino-3,6-dideoxy-D-galactose	867:916	3-(N-acetyl-L-alanyl)amino-3,6-dideoxy-D-galactose	867:916	The following structures of the O-polysaccharides were established: [Formula: see text] where R indicates H in strain G3983 or α-D-Glcp in strain G3977, d-Fuc3NAlaAc indicates 3-(N-acetyl-L-alanyl)amino-3,6-dideoxy-D-galactose.
25498203	0	25	theme	O-polysaccharides	26:42	arg1	structures					8:17	Related structures	0:17	Related structures of the O-polysaccharides of Cronobacter dublinensis G3983 and G3977	0:85	Related structures of the O-polysaccharides of Cronobacter dublinensis G3983 and G3977 containing 3-(N-acetyl-L-alanyl)amino-3,6-dideoxy-D-galactose.
25498203	4	26	theme	G3983	582:586	arg1	lipopolysaccharides					544:562	the lipopolysaccharides	540:562	the lipopolysaccharides of C. dublinensis G3983 and G3977	540:596	In this work, short-chain O-polysaccharides consisting on the average of 2-3 repeating units were obtained by mild acid or mild alkaline degradation of the lipopolysaccharides of C. dublinensis G3983 and G3977 and studied by composition analysis, Smith degradation, and (1)H and (13)C NMR spectroscopy.
25498203	5	27	theme	O-polysaccharides	723:739	arg1	structures					705:714	The following structures	691:714	The following structures of the O-polysaccharides	691:739	The following structures of the O-polysaccharides were established: [Formula: see text] where R indicates H in strain G3983 or α-D-Glcp in strain G3977, d-Fuc3NAlaAc indicates 3-(N-acetyl-L-alanyl)amino-3,6-dideoxy-D-galactose.
25498203	8	28	theme	O-antigen	1111:1119	arg1	structure					1121:1129	the O-antigen structure	1107:1129	the O-antigen structure of C. dublinensis G3983	1107:1153	The assigned gene functions are in agreement with the O-antigen structure of C. dublinensis G3983, and the side-chain glucosylation of the O-antigen of C. dublinensis G3977 is evidently encoded elsewhere in the genome.
25498203	8	29	theme	O-antigen	1196:1204	arg1	glucosylation					1175:1187	the side-chain glucosylation	1160:1187	the side-chain glucosylation of the O-antigen of C. dublinensis G3977	1160:1228	The assigned gene functions are in agreement with the O-antigen structure of C. dublinensis G3983, and the side-chain glucosylation of the O-antigen of C. dublinensis G3977 is evidently encoded elsewhere in the genome.
25498203	6	30	theme	gene	952:955	arg1	cluster					957:963	the O-antigen gene cluster	938:963	the O-antigen gene cluster	938:963	Both strains share the O-antigen gene cluster, which is identical to that of C. dublinensis O1 (Foodborne Pathog.
25498203	6	30	theme	gene	952:955	arg1	identical					975:983	identical	975:983	identical	975:983	Both strains share the O-antigen gene cluster, which is identical to that of C. dublinensis O1 (Foodborne Pathog.
25498203	2	31	theme	human	194:198	arg1	pathogens					200:208	opportunistic human pathogens	180:208	opportunistic human pathogens linked with life-threatening infections predominantly in neonates	180:274	are emerging opportunistic human pathogens linked with life-threatening infections predominantly in neonates.
25498203	4	32	dep	H	661:661	arg1	1					659:659	1	659:659	1	659:659	In this work, short-chain O-polysaccharides consisting on the average of 2-3 repeating units were obtained by mild acid or mild alkaline degradation of the lipopolysaccharides of C. dublinensis G3983 and G3977 and studied by composition analysis, Smith degradation, and (1)H and (13)C NMR spectroscopy.
25498203	8	33	theme	assigned	1061:1068	arg1	functions					1075:1083	The assigned gene functions	1057:1083	The assigned gene functions	1057:1083	The assigned gene functions are in agreement with the O-antigen structure of C. dublinensis G3983, and the side-chain glucosylation of the O-antigen of C. dublinensis G3977 is evidently encoded elsewhere in the genome.
25498203	4	34	theme	G3977	592:596	arg1	lipopolysaccharides					544:562	the lipopolysaccharides	540:562	the lipopolysaccharides of C. dublinensis G3983 and G3977	540:596	In this work, short-chain O-polysaccharides consisting on the average of 2-3 repeating units were obtained by mild acid or mild alkaline degradation of the lipopolysaccharides of C. dublinensis G3983 and G3977 and studied by composition analysis, Smith degradation, and (1)H and (13)C NMR spectroscopy.
25498203	5	35	dep	[Formula	759:766	arg1	see					769:771	see	769:771	see text	769:776	The following structures of the O-polysaccharides were established: [Formula: see text] where R indicates H in strain G3983 or α-D-Glcp in strain G3977, d-Fuc3NAlaAc indicates 3-(N-acetyl-L-alanyl)amino-3,6-dideoxy-D-galactose.
25498203	4	36	theme	units	475:479	arg1	average					450:456	the average	446:456	the average of 2-3 repeating units	446:479	In this work, short-chain O-polysaccharides consisting on the average of 2-3 repeating units were obtained by mild acid or mild alkaline degradation of the lipopolysaccharides of C. dublinensis G3983 and G3977 and studied by composition analysis, Smith degradation, and (1)H and (13)C NMR spectroscopy.
25498203	5	37	from	α-D-Glcp	818:825	arg1	strain					830:835	strain G3977	830:841	strain G3977	830:841	The following structures of the O-polysaccharides were established: [Formula: see text] where R indicates H in strain G3983 or α-D-Glcp in strain G3977, d-Fuc3NAlaAc indicates 3-(N-acetyl-L-alanyl)amino-3,6-dideoxy-D-galactose.
25498203	5	37	from	α-D-Glcp	818:825	arg1	strain					802:807	strain G3983	802:813	strain G3983	802:813	The following structures of the O-polysaccharides were established: [Formula: see text] where R indicates H in strain G3983 or α-D-Glcp in strain G3977, d-Fuc3NAlaAc indicates 3-(N-acetyl-L-alanyl)amino-3,6-dideoxy-D-galactose.
25498203	3	38	theme	important	338:346	arg1	role					348:351	an important role	335:351	an important role	335:351	O-Antigen (O-polysaccharide) is highly variable and plays an important role in virulence and niche adaptation.
25498203	4	39	theme	repeating	465:473	arg1	units					475:479	2-3 repeating units	461:479	2-3 repeating units	461:479	In this work, short-chain O-polysaccharides consisting on the average of 2-3 repeating units were obtained by mild acid or mild alkaline degradation of the lipopolysaccharides of C. dublinensis G3983 and G3977 and studied by composition analysis, Smith degradation, and (1)H and (13)C NMR spectroscopy.
25498203	6	40	theme	Foodborne	1015:1023	arg1	Pathog					1025:1030	C. dublinensis O1 (Foodborne Pathog	996:1030	C. dublinensis O1 (Foodborne Pathog	996:1030	Both strains share the O-antigen gene cluster, which is identical to that of C. dublinensis O1 (Foodborne Pathog.
25498203	5	41	theme	following	695:703	arg1	structures					705:714	The following structures	691:714	The following structures of the O-polysaccharides	691:739	The following structures of the O-polysaccharides were established: [Formula: see text] where R indicates H in strain G3983 or α-D-Glcp in strain G3977, d-Fuc3NAlaAc indicates 3-(N-acetyl-L-alanyl)amino-3,6-dideoxy-D-galactose.
25498203	7	42	dep	10	1043:1044	arg1	Dis.2013					1033:1040	Dis.2013	1033:1040	Dis.2013	1033:1040	Dis.2013, 10, 343-352).
25498203	7	42	dep	10	1043:1044	arg1	343-352					1047:1053	343-352	1047:1053	343-352	1047:1053	Dis.2013, 10, 343-352).
25498203	2	43	theme	life-threatening	222:237	arg1	infections					239:248	life-threatening infections	222:248	life-threatening infections predominantly in neonates	222:274	are emerging opportunistic human pathogens linked with life-threatening infections predominantly in neonates.
25498203	6	44	theme	O1	1011:1012	arg1	Pathog					1025:1030	C. dublinensis O1 (Foodborne Pathog	996:1030	C. dublinensis O1 (Foodborne Pathog	996:1030	Both strains share the O-antigen gene cluster, which is identical to that of C. dublinensis O1 (Foodborne Pathog.
25498203	0	45	theme	G3983	71:75	arg1	O-polysaccharides					26:42	the O-polysaccharides	22:42	the O-polysaccharides of Cronobacter dublinensis G3983 and G3977	22:85	Related structures of the O-polysaccharides of Cronobacter dublinensis G3983 and G3977 containing 3-(N-acetyl-L-alanyl)amino-3,6-dideoxy-D-galactose.
25498203	4	46	theme	lipopolysaccharides	544:562	arg1	degradation					525:535	mild alkaline degradation	511:535	mild alkaline degradation of the lipopolysaccharides of C. dublinensis G3983 and G3977	511:596	In this work, short-chain O-polysaccharides consisting on the average of 2-3 repeating units were obtained by mild acid or mild alkaline degradation of the lipopolysaccharides of C. dublinensis G3983 and G3977 and studied by composition analysis, Smith degradation, and (1)H and (13)C NMR spectroscopy.
25498203	4	46	theme	lipopolysaccharides	544:562	arg1	acid					503:506	mild acid	498:506	mild acid	498:506	In this work, short-chain O-polysaccharides consisting on the average of 2-3 repeating units were obtained by mild acid or mild alkaline degradation of the lipopolysaccharides of C. dublinensis G3983 and G3977 and studied by composition analysis, Smith degradation, and (1)H and (13)C NMR spectroscopy.
25498203	8	47	with	agreement	1092:1100	arg1	structure					1121:1129	the O-antigen structure	1107:1129	the O-antigen structure of C. dublinensis G3983	1107:1153	The assigned gene functions are in agreement with the O-antigen structure of C. dublinensis G3983, and the side-chain glucosylation of the O-antigen of C. dublinensis G3977 is evidently encoded elsewhere in the genome.
25498203	6	48	theme	dublinensis	999:1009	arg1	Pathog					1025:1030	C. dublinensis O1 (Foodborne Pathog	996:1030	C. dublinensis O1 (Foodborne Pathog	996:1030	Both strains share the O-antigen gene cluster, which is identical to that of C. dublinensis O1 (Foodborne Pathog.
28482536	0	0	theme	antibacterial	83:95	arg1	efficacy					97:104	antibacterial efficacy	83:104	antibacterial efficacy	83:104	Influences of mesoporous zinc-calcium silicate on water absorption, degradability, antibacterial efficacy, hemostatic performances and cell viability to microporous starch based hemostat.
28482536	6	1	from	model	1106:1110	arg1	arterial					1142:1149	arterial	1142:1149	arterial	1142:1149	In addition, for the animal model of rabbits in ear vein, skin, arterial and liver injuries, the hemostatic time of the composites obviously reduced with the increase of m-ZSC content, in which the composite with 15wt% m-ZCS content (15mZSC) showed remarkable efficacy on bleeding control.
28482536	6	1	from	model	1106:1110	arg1	injuries					1161:1168	liver injuries	1155:1168	liver injuries	1155:1168	In addition, for the animal model of rabbits in ear vein, skin, arterial and liver injuries, the hemostatic time of the composites obviously reduced with the increase of m-ZSC content, in which the composite with 15wt% m-ZCS content (15mZSC) showed remarkable efficacy on bleeding control.
28482536	6	1	from	model	1106:1110	arg1	skin					1136:1139	skin	1136:1139	skin	1136:1139	In addition, for the animal model of rabbits in ear vein, skin, arterial and liver injuries, the hemostatic time of the composites obviously reduced with the increase of m-ZSC content, in which the composite with 15wt% m-ZCS content (15mZSC) showed remarkable efficacy on bleeding control.
28482536	6	1	from	model	1106:1110	arg1	vein					1130:1133	ear vein	1126:1133	ear vein	1126:1133	In addition, for the animal model of rabbits in ear vein, skin, arterial and liver injuries, the hemostatic time of the composites obviously reduced with the increase of m-ZSC content, in which the composite with 15wt% m-ZCS content (15mZSC) showed remarkable efficacy on bleeding control.
28482536	3	2	dep	absorption	587:596	arg1	the					577:579	the	577:579	the	577:579	The results showed that the incorporation of m-ZCS into MS significantly enhanced the water absorption and degradability of the composites, which were dependent on the m-ZCS content.
28482536	1	3	theme	large	278:282	arg1	vessel					284:289	large vessel	278:289	large vessel	278:289	Efficacious hemostatic agents have significant potential application in visceral organ or large vessel arterial injure.
28482536	6	4	theme	remarkable	1327:1336	arg1	efficacy					1338:1345	remarkable efficacy	1327:1345	remarkable efficacy	1327:1345	In addition, for the animal model of rabbits in ear vein, skin, arterial and liver injuries, the hemostatic time of the composites obviously reduced with the increase of m-ZSC content, in which the composite with 15wt% m-ZCS content (15mZSC) showed remarkable efficacy on bleeding control.
28482536	8	5	with	composites	1512:1521	arg1	properties					1567:1576	excellent hemostatic and antibacterial properties	1528:1576	excellent hemostatic and antibacterial properties	1528:1576	The results suggested that the m-ZCS/MS composites with excellent hemostatic and antibacterial properties might be a candidate for controlling bleeding and infection.
28482536	4	6	theme	antibacterial	708:720	arg1	property					722:729	antibacterial property	708:729	antibacterial property	708:729	Moreover, the composites with antibacterial property could inhibit the growth of Escherichia coli (E. coli) and the antibacterial ratios increased with the m-ZCS content.
28482536	6	7	theme	animal	1099:1104	arg1	model					1106:1110	the animal model	1095:1110	the animal model of rabbits in ear vein, skin, arterial and liver injuries	1095:1168	In addition, for the animal model of rabbits in ear vein, skin, arterial and liver injuries, the hemostatic time of the composites obviously reduced with the increase of m-ZSC content, in which the composite with 15wt% m-ZCS content (15mZSC) showed remarkable efficacy on bleeding control.
28482536	6	8	theme	content	1254:1260	arg1	increase					1236:1243	the increase	1232:1243	the increase	1232:1243	In addition, for the animal model of rabbits in ear vein, skin, arterial and liver injuries, the hemostatic time of the composites obviously reduced with the increase of m-ZSC content, in which the composite with 15wt% m-ZCS content (15mZSC) showed remarkable efficacy on bleeding control.
28482536	5	9	dep	in	853:854	arg1	vitro					856:860	vitro	856:860	vitro	856:860	The in vitro coagulation evaluation by using activated partial thromboplastin time (APTT) and prothrombin time (PT) revealed that the composites significantly activated the intrinsic and extrinsic pathway of coagulation cascade.
28482536	4	10	theme	coli	771:774	arg1	growth					749:754	the growth	745:754	the growth of Escherichia coli (E. coli)	745:784	Moreover, the composites with antibacterial property could inhibit the growth of Escherichia coli (E. coli) and the antibacterial ratios increased with the m-ZCS content.
28482536	1	11	theme	arterial	291:298	arg1	organ					269:273	visceral organ	260:273	visceral organ	260:273	Efficacious hemostatic agents have significant potential application in visceral organ or large vessel arterial injure.
28482536	7	12	theme	cells	1419:1423	arg1	viability					1401:1409	the viability	1397:1409	the viability of L929 cells	1397:1423	The composites could promote the viability of L929 cells, indicating no cytotoxicity of the composites.
28482536	6	13	theme	m-ZSC	1248:1252	arg1	content					1254:1260	m-ZSC content	1248:1260	m-ZSC content	1248:1260	In addition, for the animal model of rabbits in ear vein, skin, arterial and liver injuries, the hemostatic time of the composites obviously reduced with the increase of m-ZSC content, in which the composite with 15wt% m-ZCS content (15mZSC) showed remarkable efficacy on bleeding control.
28482536	5	14	theme	thromboplastin	912:925	arg1	APTT					933:936	APTT	933:936	APTT	933:936	The in vitro coagulation evaluation by using activated partial thromboplastin time (APTT) and prothrombin time (PT) revealed that the composites significantly activated the intrinsic and extrinsic pathway of coagulation cascade.
28482536	5	14	theme	thromboplastin	912:925	arg1	time					927:930	activated partial thromboplastin time	894:930	activated partial thromboplastin time (APTT)	894:937	The in vitro coagulation evaluation by using activated partial thromboplastin time (APTT) and prothrombin time (PT) revealed that the composites significantly activated the intrinsic and extrinsic pathway of coagulation cascade.
28482536	5	15	theme	in	853:854	arg1	evaluation					874:883	The in vitro coagulation evaluation	849:883	The in vitro coagulation evaluation by using activated partial thromboplastin time (APTT) and prothrombin time (PT)	849:963	The in vitro coagulation evaluation by using activated partial thromboplastin time (APTT) and prothrombin time (PT) revealed that the composites significantly activated the intrinsic and extrinsic pathway of coagulation cascade.
28482536	6	16	theme	liver	1155:1159	arg1	injuries					1161:1168	liver injuries	1155:1168	liver injuries	1155:1168	In addition, for the animal model of rabbits in ear vein, skin, arterial and liver injuries, the hemostatic time of the composites obviously reduced with the increase of m-ZSC content, in which the composite with 15wt% m-ZCS content (15mZSC) showed remarkable efficacy on bleeding control.
28482536	5	17	theme	coagulation	862:872	arg1	evaluation					874:883	The in vitro coagulation evaluation	849:883	The in vitro coagulation evaluation by using activated partial thromboplastin time (APTT) and prothrombin time (PT)	849:963	The in vitro coagulation evaluation by using activated partial thromboplastin time (APTT) and prothrombin time (PT) revealed that the composites significantly activated the intrinsic and extrinsic pathway of coagulation cascade.
28482536	0	18	theme	hemostatic	107:116	arg1	performances					118:129	hemostatic performances	107:129	hemostatic performances	107:129	Influences of mesoporous zinc-calcium silicate on water absorption, degradability, antibacterial efficacy, hemostatic performances and cell viability to microporous starch based hemostat.
28482536	7	19	theme	L929	1414:1417	arg1	cells					1419:1423	L929 cells	1414:1423	L929 cells	1414:1423	The composites could promote the viability of L929 cells, indicating no cytotoxicity of the composites.
28482536	1	20	theme	Efficacious	188:198	arg1	agents					211:216	Efficacious hemostatic agents	188:216	Efficacious hemostatic agents	188:216	Efficacious hemostatic agents have significant potential application in visceral organ or large vessel arterial injure.
28482536	2	21	theme	hemorrhage	460:469	arg1	control					471:477	hemorrhage control	460:477	hemorrhage control	460:477	In this study, mesoporous zinc-calcium silicate (m-ZCS) was synthesized, and microporous starch (MS) based hemostatic agents of m-ZCS/MS composites for hemorrhage control was fabricated.
28482536	4	22	with	composites	692:701	arg1	property					722:729	antibacterial property	708:729	antibacterial property	708:729	Moreover, the composites with antibacterial property could inhibit the growth of Escherichia coli (E. coli) and the antibacterial ratios increased with the m-ZCS content.
28482536	5	23	theme	prothrombin	943:953	arg1	PT					961:962	PT	961:962	PT	961:962	The in vitro coagulation evaluation by using activated partial thromboplastin time (APTT) and prothrombin time (PT) revealed that the composites significantly activated the intrinsic and extrinsic pathway of coagulation cascade.
28482536	5	23	theme	prothrombin	943:953	arg1	time					955:958	prothrombin time	943:958	prothrombin time (PT)	943:963	The in vitro coagulation evaluation by using activated partial thromboplastin time (APTT) and prothrombin time (PT) revealed that the composites significantly activated the intrinsic and extrinsic pathway of coagulation cascade.
28482536	6	24	from	injuries	1161:1168	arg1	model					1106:1110	the animal model	1095:1110	the animal model of rabbits in ear vein, skin, arterial and liver injuries	1095:1168	In addition, for the animal model of rabbits in ear vein, skin, arterial and liver injuries, the hemostatic time of the composites obviously reduced with the increase of m-ZSC content, in which the composite with 15wt% m-ZCS content (15mZSC) showed remarkable efficacy on bleeding control.
28482536	1	25	theme	hemostatic	200:209	arg1	agents					211:216	Efficacious hemostatic agents	188:216	Efficacious hemostatic agents	188:216	Efficacious hemostatic agents have significant potential application in visceral organ or large vessel arterial injure.
28482536	7	26	theme	composites	1460:1469	arg1	cytotoxicity					1440:1451	no cytotoxicity	1437:1451	no cytotoxicity of the composites	1437:1469	The composites could promote the viability of L929 cells, indicating no cytotoxicity of the composites.
28482536	2	27	theme	zinc-calcium	334:345	arg1	m-ZCS					357:361	m-ZCS	357:361	m-ZCS	357:361	In this study, mesoporous zinc-calcium silicate (m-ZCS) was synthesized, and microporous starch (MS) based hemostatic agents of m-ZCS/MS composites for hemorrhage control was fabricated.
28482536	2	27	theme	zinc-calcium	334:345	arg1	silicate					347:354	mesoporous zinc-calcium silicate	323:354	mesoporous zinc-calcium silicate (m-ZCS)	323:362	In this study, mesoporous zinc-calcium silicate (m-ZCS) was synthesized, and microporous starch (MS) based hemostatic agents of m-ZCS/MS composites for hemorrhage control was fabricated.
28482536	6	28	from	skin	1136:1139	arg1	model					1106:1110	the animal model	1095:1110	the animal model of rabbits in ear vein, skin, arterial and liver injuries	1095:1168	In addition, for the animal model of rabbits in ear vein, skin, arterial and liver injuries, the hemostatic time of the composites obviously reduced with the increase of m-ZSC content, in which the composite with 15wt% m-ZCS content (15mZSC) showed remarkable efficacy on bleeding control.
28482536	2	29	theme	composites	445:454	arg1	agents					426:431	hemostatic agents	415:431	hemostatic agents of m-ZCS/MS composites for hemorrhage control	415:477	In this study, mesoporous zinc-calcium silicate (m-ZCS) was synthesized, and microporous starch (MS) based hemostatic agents of m-ZCS/MS composites for hemorrhage control was fabricated.
28482536	8	30	theme	m-ZCS/MS	1503:1510	arg1	candidate					1589:1597	a candidate	1587:1597	a candidate for controlling bleeding and infection	1587:1636	The results suggested that the m-ZCS/MS composites with excellent hemostatic and antibacterial properties might be a candidate for controlling bleeding and infection.
28482536	8	30	theme	m-ZCS/MS	1503:1510	arg1	composites					1512:1521	the m-ZCS/MS composites	1499:1521	the m-ZCS/MS composites with excellent hemostatic and antibacterial properties	1499:1576	The results suggested that the m-ZCS/MS composites with excellent hemostatic and antibacterial properties might be a candidate for controlling bleeding and infection.
28482536	0	31	theme	cell	135:138	arg1	viability					140:148	cell viability	135:148	cell viability	135:148	Influences of mesoporous zinc-calcium silicate on water absorption, degradability, antibacterial efficacy, hemostatic performances and cell viability to microporous starch based hemostat.
28482536	2	32	theme	mesoporous	323:332	arg1	m-ZCS					357:361	m-ZCS	357:361	m-ZCS	357:361	In this study, mesoporous zinc-calcium silicate (m-ZCS) was synthesized, and microporous starch (MS) based hemostatic agents of m-ZCS/MS composites for hemorrhage control was fabricated.
28482536	2	32	theme	mesoporous	323:332	arg1	silicate					347:354	mesoporous zinc-calcium silicate	323:354	mesoporous zinc-calcium silicate (m-ZCS)	323:362	In this study, mesoporous zinc-calcium silicate (m-ZCS) was synthesized, and microporous starch (MS) based hemostatic agents of m-ZCS/MS composites for hemorrhage control was fabricated.
28482536	2	33	theme	m-ZCS/MS	436:443	arg1	composites					445:454	m-ZCS/MS composites	436:454	m-ZCS/MS composites	436:454	In this study, mesoporous zinc-calcium silicate (m-ZCS) was synthesized, and microporous starch (MS) based hemostatic agents of m-ZCS/MS composites for hemorrhage control was fabricated.
28482536	8	34	theme	excellent	1528:1536	arg1	properties					1567:1576	excellent hemostatic and antibacterial properties	1528:1576	excellent hemostatic and antibacterial properties	1528:1576	The results suggested that the m-ZCS/MS composites with excellent hemostatic and antibacterial properties might be a candidate for controlling bleeding and infection.
28482536	6	35	theme	m-ZCS	1297:1301	arg1	15mZSC					1312:1317	15mZSC	1312:1317	15mZSC	1312:1317	In addition, for the animal model of rabbits in ear vein, skin, arterial and liver injuries, the hemostatic time of the composites obviously reduced with the increase of m-ZSC content, in which the composite with 15wt% m-ZCS content (15mZSC) showed remarkable efficacy on bleeding control.
28482536	6	35	theme	m-ZCS	1297:1301	arg1	content					1303:1309	15wt% m-ZCS content	1291:1309	15wt% m-ZCS content (15mZSC)	1291:1318	In addition, for the animal model of rabbits in ear vein, skin, arterial and liver injuries, the hemostatic time of the composites obviously reduced with the increase of m-ZSC content, in which the composite with 15wt% m-ZCS content (15mZSC) showed remarkable efficacy on bleeding control.
28482536	3	36	theme	m-ZCS	663:667	arg1	content					669:675	the m-ZCS content	659:675	the m-ZCS content	659:675	The results showed that the incorporation of m-ZCS into MS significantly enhanced the water absorption and degradability of the composites, which were dependent on the m-ZCS content.
28482536	3	37	theme	water	581:585	arg1	absorption					587:596	water absorption	581:596	water absorption	581:596	The results showed that the incorporation of m-ZCS into MS significantly enhanced the water absorption and degradability of the composites, which were dependent on the m-ZCS content.
28482536	6	38	from	arterial	1142:1149	arg1	model					1106:1110	the animal model	1095:1110	the animal model of rabbits in ear vein, skin, arterial and liver injuries	1095:1168	In addition, for the animal model of rabbits in ear vein, skin, arterial and liver injuries, the hemostatic time of the composites obviously reduced with the increase of m-ZSC content, in which the composite with 15wt% m-ZCS content (15mZSC) showed remarkable efficacy on bleeding control.
28482536	1	39	theme	significant	223:233	arg1	application					245:255	significant potential application	223:255	significant potential application	223:255	Efficacious hemostatic agents have significant potential application in visceral organ or large vessel arterial injure.
28482536	6	40	theme	ear	1126:1128	arg1	vein					1130:1133	ear vein	1126:1133	ear vein	1126:1133	In addition, for the animal model of rabbits in ear vein, skin, arterial and liver injuries, the hemostatic time of the composites obviously reduced with the increase of m-ZSC content, in which the composite with 15wt% m-ZCS content (15mZSC) showed remarkable efficacy on bleeding control.
28482536	6	41	theme	%	1295:1295	arg1	15mZSC					1312:1317	15mZSC	1312:1317	15mZSC	1312:1317	In addition, for the animal model of rabbits in ear vein, skin, arterial and liver injuries, the hemostatic time of the composites obviously reduced with the increase of m-ZSC content, in which the composite with 15wt% m-ZCS content (15mZSC) showed remarkable efficacy on bleeding control.
28482536	6	41	theme	%	1295:1295	arg1	content					1303:1309	15wt% m-ZCS content	1291:1309	15wt% m-ZCS content (15mZSC)	1291:1318	In addition, for the animal model of rabbits in ear vein, skin, arterial and liver injuries, the hemostatic time of the composites obviously reduced with the increase of m-ZSC content, in which the composite with 15wt% m-ZCS content (15mZSC) showed remarkable efficacy on bleeding control.
28482536	2	42	theme	hemostatic	415:424	arg1	agents					426:431	hemostatic agents	415:431	hemostatic agents of m-ZCS/MS composites for hemorrhage control	415:477	In this study, mesoporous zinc-calcium silicate (m-ZCS) was synthesized, and microporous starch (MS) based hemostatic agents of m-ZCS/MS composites for hemorrhage control was fabricated.
28482536	5	43	theme	intrinsic	1022:1030	arg1	pathway					1046:1052	the intrinsic and extrinsic pathway	1018:1052	the intrinsic and extrinsic pathway of coagulation cascade	1018:1075	The in vitro coagulation evaluation by using activated partial thromboplastin time (APTT) and prothrombin time (PT) revealed that the composites significantly activated the intrinsic and extrinsic pathway of coagulation cascade.
28482536	6	44	with	composite	1276:1284	arg1	15mZSC					1312:1317	15mZSC	1312:1317	15mZSC	1312:1317	In addition, for the animal model of rabbits in ear vein, skin, arterial and liver injuries, the hemostatic time of the composites obviously reduced with the increase of m-ZSC content, in which the composite with 15wt% m-ZCS content (15mZSC) showed remarkable efficacy on bleeding control.
28482536	6	44	with	composite	1276:1284	arg1	content					1303:1309	15wt% m-ZCS content	1291:1309	15wt% m-ZCS content (15mZSC)	1291:1318	In addition, for the animal model of rabbits in ear vein, skin, arterial and liver injuries, the hemostatic time of the composites obviously reduced with the increase of m-ZSC content, in which the composite with 15wt% m-ZCS content (15mZSC) showed remarkable efficacy on bleeding control.
28482536	1	45	theme	potential	235:243	arg1	application					245:255	significant potential application	223:255	significant potential application	223:255	Efficacious hemostatic agents have significant potential application in visceral organ or large vessel arterial injure.
28482536	6	46	theme	15wt	1291:1294	arg1	15mZSC					1312:1317	15mZSC	1312:1317	15mZSC	1312:1317	In addition, for the animal model of rabbits in ear vein, skin, arterial and liver injuries, the hemostatic time of the composites obviously reduced with the increase of m-ZSC content, in which the composite with 15wt% m-ZCS content (15mZSC) showed remarkable efficacy on bleeding control.
28482536	6	46	theme	15wt	1291:1294	arg1	content					1303:1309	15wt% m-ZCS content	1291:1309	15wt% m-ZCS content (15mZSC)	1291:1318	In addition, for the animal model of rabbits in ear vein, skin, arterial and liver injuries, the hemostatic time of the composites obviously reduced with the increase of m-ZSC content, in which the composite with 15wt% m-ZCS content (15mZSC) showed remarkable efficacy on bleeding control.
28482536	0	47	theme	zinc-calcium	25:36	arg1	Influences					0:9	Influences	0:9	Influences of mesoporous zinc-calcium	0:36	Influences of mesoporous zinc-calcium silicate on water absorption, degradability, antibacterial efficacy, hemostatic performances and cell viability to microporous starch based hemostat.
28482536	5	48	theme	activated	894:902	arg1	APTT					933:936	APTT	933:936	APTT	933:936	The in vitro coagulation evaluation by using activated partial thromboplastin time (APTT) and prothrombin time (PT) revealed that the composites significantly activated the intrinsic and extrinsic pathway of coagulation cascade.
28482536	5	48	theme	activated	894:902	arg1	time					927:930	activated partial thromboplastin time	894:930	activated partial thromboplastin time (APTT)	894:937	The in vitro coagulation evaluation by using activated partial thromboplastin time (APTT) and prothrombin time (PT) revealed that the composites significantly activated the intrinsic and extrinsic pathway of coagulation cascade.
28482536	0	49	theme	starch	165:170	arg1	hemostat					178:185	microporous starch based hemostat	153:185	microporous starch based hemostat	153:185	Influences of mesoporous zinc-calcium silicate on water absorption, degradability, antibacterial efficacy, hemostatic performances and cell viability to microporous starch based hemostat.
28482536	5	50	theme	extrinsic	1036:1044	arg1	pathway					1046:1052	the intrinsic and extrinsic pathway	1018:1052	the intrinsic and extrinsic pathway of coagulation cascade	1018:1075	The in vitro coagulation evaluation by using activated partial thromboplastin time (APTT) and prothrombin time (PT) revealed that the composites significantly activated the intrinsic and extrinsic pathway of coagulation cascade.
28482536	6	51	from	rabbits	1115:1121	arg1	arterial					1142:1149	arterial	1142:1149	arterial	1142:1149	In addition, for the animal model of rabbits in ear vein, skin, arterial and liver injuries, the hemostatic time of the composites obviously reduced with the increase of m-ZSC content, in which the composite with 15wt% m-ZCS content (15mZSC) showed remarkable efficacy on bleeding control.
28482536	6	51	from	rabbits	1115:1121	arg1	injuries					1161:1168	liver injuries	1155:1168	liver injuries	1155:1168	In addition, for the animal model of rabbits in ear vein, skin, arterial and liver injuries, the hemostatic time of the composites obviously reduced with the increase of m-ZSC content, in which the composite with 15wt% m-ZCS content (15mZSC) showed remarkable efficacy on bleeding control.
28482536	6	51	from	rabbits	1115:1121	arg1	skin					1136:1139	skin	1136:1139	skin	1136:1139	In addition, for the animal model of rabbits in ear vein, skin, arterial and liver injuries, the hemostatic time of the composites obviously reduced with the increase of m-ZSC content, in which the composite with 15wt% m-ZCS content (15mZSC) showed remarkable efficacy on bleeding control.
28482536	6	51	from	rabbits	1115:1121	arg1	vein					1130:1133	ear vein	1126:1133	ear vein	1126:1133	In addition, for the animal model of rabbits in ear vein, skin, arterial and liver injuries, the hemostatic time of the composites obviously reduced with the increase of m-ZSC content, in which the composite with 15wt% m-ZCS content (15mZSC) showed remarkable efficacy on bleeding control.
28482536	0	52	theme	water	50:54	arg1	absorption					56:65	water absorption	50:65	water absorption	50:65	Influences of mesoporous zinc-calcium silicate on water absorption, degradability, antibacterial efficacy, hemostatic performances and cell viability to microporous starch based hemostat.
28482536	0	53	theme	microporous	153:163	arg1	hemostat					178:185	microporous starch based hemostat	153:185	microporous starch based hemostat	153:185	Influences of mesoporous zinc-calcium silicate on water absorption, degradability, antibacterial efficacy, hemostatic performances and cell viability to microporous starch based hemostat.
28482536	1	54	dep	arterial	291:298	arg1	injure					300:305	injure	300:305	injure	300:305	Efficacious hemostatic agents have significant potential application in visceral organ or large vessel arterial injure.
28482536	4	55	theme	m-ZCS	834:838	arg1	content					840:846	the m-ZCS content	830:846	the m-ZCS content	830:846	Moreover, the composites with antibacterial property could inhibit the growth of Escherichia coli (E. coli) and the antibacterial ratios increased with the m-ZCS content.
28482536	6	56	from	vein	1130:1133	arg1	model					1106:1110	the animal model	1095:1110	the animal model of rabbits in ear vein, skin, arterial and liver injuries	1095:1168	In addition, for the animal model of rabbits in ear vein, skin, arterial and liver injuries, the hemostatic time of the composites obviously reduced with the increase of m-ZSC content, in which the composite with 15wt% m-ZCS content (15mZSC) showed remarkable efficacy on bleeding control.
28482536	4	57	dep	coli	771:774	arg1	coli					780:783	E. coli	777:783	E. coli	777:783	Moreover, the composites with antibacterial property could inhibit the growth of Escherichia coli (E. coli) and the antibacterial ratios increased with the m-ZCS content.
28482536	8	58	theme	antibacterial	1553:1565	arg1	properties					1567:1576	excellent hemostatic and antibacterial properties	1528:1576	excellent hemostatic and antibacterial properties	1528:1576	The results suggested that the m-ZCS/MS composites with excellent hemostatic and antibacterial properties might be a candidate for controlling bleeding and infection.
28482536	5	59	theme	coagulation	1057:1067	arg1	cascade					1069:1075	coagulation cascade	1057:1075	coagulation cascade	1057:1075	The in vitro coagulation evaluation by using activated partial thromboplastin time (APTT) and prothrombin time (PT) revealed that the composites significantly activated the intrinsic and extrinsic pathway of coagulation cascade.
28482536	4	60	theme	antibacterial	794:806	arg1	ratios					808:813	the antibacterial ratios	790:813	the antibacterial ratios	790:813	Moreover, the composites with antibacterial property could inhibit the growth of Escherichia coli (E. coli) and the antibacterial ratios increased with the m-ZCS content.
28482536	3	61	theme	composites	623:632	arg1	degradability					602:614	degradability	602:614	degradability	602:614	The results showed that the incorporation of m-ZCS into MS significantly enhanced the water absorption and degradability of the composites, which were dependent on the m-ZCS content.
28482536	3	61	theme	composites	623:632	arg1	absorption					587:596	water absorption	581:596	water absorption	581:596	The results showed that the incorporation of m-ZCS into MS significantly enhanced the water absorption and degradability of the composites, which were dependent on the m-ZCS content.
28482536	6	62	theme	rabbits	1115:1121	arg1	model					1106:1110	the animal model	1095:1110	the animal model of rabbits in ear vein, skin, arterial and liver injuries	1095:1168	In addition, for the animal model of rabbits in ear vein, skin, arterial and liver injuries, the hemostatic time of the composites obviously reduced with the increase of m-ZSC content, in which the composite with 15wt% m-ZCS content (15mZSC) showed remarkable efficacy on bleeding control.
28482536	0	63	theme	based	172:176	arg1	hemostat					178:185	microporous starch based hemostat	153:185	microporous starch based hemostat	153:185	Influences of mesoporous zinc-calcium silicate on water absorption, degradability, antibacterial efficacy, hemostatic performances and cell viability to microporous starch based hemostat.
28482536	5	64	theme	cascade	1069:1075	arg1	pathway					1046:1052	the intrinsic and extrinsic pathway	1018:1052	the intrinsic and extrinsic pathway of coagulation cascade	1018:1075	The in vitro coagulation evaluation by using activated partial thromboplastin time (APTT) and prothrombin time (PT) revealed that the composites significantly activated the intrinsic and extrinsic pathway of coagulation cascade.
28482536	5	65	theme	partial	904:910	arg1	APTT					933:936	APTT	933:936	APTT	933:936	The in vitro coagulation evaluation by using activated partial thromboplastin time (APTT) and prothrombin time (PT) revealed that the composites significantly activated the intrinsic and extrinsic pathway of coagulation cascade.
28482536	5	65	theme	partial	904:910	arg1	time					927:930	activated partial thromboplastin time	894:930	activated partial thromboplastin time (APTT)	894:937	The in vitro coagulation evaluation by using activated partial thromboplastin time (APTT) and prothrombin time (PT) revealed that the composites significantly activated the intrinsic and extrinsic pathway of coagulation cascade.
28482536	1	66	theme	visceral	260:267	arg1	organ					269:273	visceral organ	260:273	visceral organ	260:273	Efficacious hemostatic agents have significant potential application in visceral organ or large vessel arterial injure.
28482536	6	67	theme	bleeding	1350:1357	arg1	control					1359:1365	bleeding control	1350:1365	bleeding control	1350:1365	In addition, for the animal model of rabbits in ear vein, skin, arterial and liver injuries, the hemostatic time of the composites obviously reduced with the increase of m-ZSC content, in which the composite with 15wt% m-ZCS content (15mZSC) showed remarkable efficacy on bleeding control.
28482536	1	68	contain	have	218:221	arg2	application					245:255	significant potential application	223:255	significant potential application	223:255	Efficacious hemostatic agents have significant potential application in visceral organ or large vessel arterial injure.
28482536	1	68	contain	have	218:221	arg1	agents					211:216	Efficacious hemostatic agents	188:216	Efficacious hemostatic agents	188:216	Efficacious hemostatic agents have significant potential application in visceral organ or large vessel arterial injure.
28482536	6	69	theme	hemostatic	1175:1184	arg1	time					1186:1189	the hemostatic time	1171:1189	the hemostatic time of the composites	1171:1207	In addition, for the animal model of rabbits in ear vein, skin, arterial and liver injuries, the hemostatic time of the composites obviously reduced with the increase of m-ZSC content, in which the composite with 15wt% m-ZCS content (15mZSC) showed remarkable efficacy on bleeding control.
28482536	6	70	theme	composites	1198:1207	arg1	time					1186:1189	the hemostatic time	1171:1189	the hemostatic time of the composites	1171:1207	In addition, for the animal model of rabbits in ear vein, skin, arterial and liver injuries, the hemostatic time of the composites obviously reduced with the increase of m-ZSC content, in which the composite with 15wt% m-ZCS content (15mZSC) showed remarkable efficacy on bleeding control.
28482536	2	71	theme	microporous	385:395	arg1	MS					405:406	MS	405:406	MS	405:406	In this study, mesoporous zinc-calcium silicate (m-ZCS) was synthesized, and microporous starch (MS) based hemostatic agents of m-ZCS/MS composites for hemorrhage control was fabricated.
28482536	2	71	theme	microporous	385:395	arg1	starch					397:402	microporous starch	385:402	microporous starch (MS) based hemostatic agents of m-ZCS/MS composites for hemorrhage control	385:477	In this study, mesoporous zinc-calcium silicate (m-ZCS) was synthesized, and microporous starch (MS) based hemostatic agents of m-ZCS/MS composites for hemorrhage control was fabricated.
28482536	0	72	theme	mesoporous	14:23	arg1	zinc-calcium					25:36	mesoporous zinc-calcium	14:36	mesoporous zinc-calcium	14:36	Influences of mesoporous zinc-calcium silicate on water absorption, degradability, antibacterial efficacy, hemostatic performances and cell viability to microporous starch based hemostat.
28482536	3	73	theme	m-ZCS	540:544	arg1	incorporation					523:535	the incorporation	519:535	the incorporation of m-ZCS into MS	519:552	The results showed that the incorporation of m-ZCS into MS significantly enhanced the water absorption and degradability of the composites, which were dependent on the m-ZCS content.
28482536	8	74	theme	hemostatic	1538:1547	arg1	properties					1567:1576	excellent hemostatic and antibacterial properties	1528:1576	excellent hemostatic and antibacterial properties	1528:1576	The results suggested that the m-ZCS/MS composites with excellent hemostatic and antibacterial properties might be a candidate for controlling bleeding and infection.
26876821	5	0	theme	secretion	802:810	arg1	productions					759:769	the productions	755:769	the productions of IFN-γ, IL-2, IL-6 and TNF-α secretion in the peripheral lymphocytes	755:840	They all could cause the proliferation of the lymphocyte, up-regulate stimulate the productions of IFN-γ, IL-2, IL-6 and TNF-α secretion in the peripheral lymphocytes.
26876821	7	1	theme	different	1053:1061	arg1	characteristics					1074:1088	different structural characteristics	1053:1088	different structural characteristics	1053:1088	These indicated that five CAPSs displayed different activities which were associated with their different structural characteristics and CAPS70, with the molecular weights of 20.82kDa and consisting of mannose and glucose in the molar ratio of 1.20:1.01, possessed the strongest immuno-enhancement activity.
26876821	4	2	theme	different	610:618	arg1	features					631:638	different structural features	610:638	different structural features	610:638	The results showed that they displayed different structural features and immuno-enhancement activities.
26876821	0	3	theme	Angelica	90:97	arg1	sinensis					99:106	Angelica sinensis	90:106	Angelica sinensis	90:106	Structural analysis and immunoregulation activity comparison of five polysaccharides from Angelica sinensis.
26876821	0	4	from	analysis	11:18	arg1	sinensis					99:106	Angelica sinensis	90:106	Angelica sinensis	90:106	Structural analysis and immunoregulation activity comparison of five polysaccharides from Angelica sinensis.
26876821	7	5	theme	consisting	1145:1154	arg1	weights					1121:1127	the molecular weights	1107:1127	the molecular weights of 20.82kDa and consisting of mannose and glucose	1107:1177	These indicated that five CAPSs displayed different activities which were associated with their different structural characteristics and CAPS70, with the molecular weights of 20.82kDa and consisting of mannose and glucose in the molar ratio of 1.20:1.01, possessed the strongest immuno-enhancement activity.
26876821	6	6	theme	CD56	927:930	arg1	cells					935:939	CD3(+)CD56(+) cells	921:939	CD3(+)CD56(+) cells	921:939	Further experiments showed that CAPS50 and CAPS70 could increase the ratio of CD3(+)CD56(+) cells to some extent.
26876821	2	7	theme	transform-infrared	293:310	arg1	FT-IR					326:330	FT-IR	326:330	FT-IR	326:330	Their structures were identified by Fourier transform-infrared spectroscopy (FT-IR), molecular weights were evaluated by high performance gel permeation chromatography (HPGPC) and compositions were analyzed by gas chromatography-mass spectrometry (GC-MS).
26876821	2	7	theme	transform-infrared	293:310	arg1	spectroscopy					312:323	Fourier transform-infrared spectroscopy	285:323	Fourier transform-infrared spectroscopy (FT-IR)	285:331	Their structures were identified by Fourier transform-infrared spectroscopy (FT-IR), molecular weights were evaluated by high performance gel permeation chromatography (HPGPC) and compositions were analyzed by gas chromatography-mass spectrometry (GC-MS).
26876821	5	8	theme	IL-2	781:784	arg1	productions					759:769	the productions	755:769	the productions of IFN-γ, IL-2, IL-6 and TNF-α secretion in the peripheral lymphocytes	755:840	They all could cause the proliferation of the lymphocyte, up-regulate stimulate the productions of IFN-γ, IL-2, IL-6 and TNF-α secretion in the peripheral lymphocytes.
26876821	7	9	theme	20.82kDa	1132:1139	arg1	weights					1121:1127	the molecular weights	1107:1127	the molecular weights of 20.82kDa and consisting of mannose and glucose	1107:1177	These indicated that five CAPSs displayed different activities which were associated with their different structural characteristics and CAPS70, with the molecular weights of 20.82kDa and consisting of mannose and glucose in the molar ratio of 1.20:1.01, possessed the strongest immuno-enhancement activity.
26876821	2	10	theme	Fourier	285:291	arg1	FT-IR					326:330	FT-IR	326:330	FT-IR	326:330	Their structures were identified by Fourier transform-infrared spectroscopy (FT-IR), molecular weights were evaluated by high performance gel permeation chromatography (HPGPC) and compositions were analyzed by gas chromatography-mass spectrometry (GC-MS).
26876821	2	10	theme	Fourier	285:291	arg1	spectroscopy					312:323	Fourier transform-infrared spectroscopy	285:323	Fourier transform-infrared spectroscopy (FT-IR)	285:331	Their structures were identified by Fourier transform-infrared spectroscopy (FT-IR), molecular weights were evaluated by high performance gel permeation chromatography (HPGPC) and compositions were analyzed by gas chromatography-mass spectrometry (GC-MS).
26876821	4	11	theme	structural	620:629	arg1	features					631:638	different structural features	610:638	different structural features	610:638	The results showed that they displayed different structural features and immuno-enhancement activities.
26876821	7	12	theme	molecular	1111:1119	arg1	weights					1121:1127	the molecular weights	1107:1127	the molecular weights of 20.82kDa and consisting of mannose and glucose	1107:1177	These indicated that five CAPSs displayed different activities which were associated with their different structural characteristics and CAPS70, with the molecular weights of 20.82kDa and consisting of mannose and glucose in the molar ratio of 1.20:1.01, possessed the strongest immuno-enhancement activity.
26876821	6	13	theme	Further	843:849	arg1	experiments					851:861	Further experiments	843:861	Further experiments	843:861	Further experiments showed that CAPS50 and CAPS70 could increase the ratio of CD3(+)CD56(+) cells to some extent.
26876821	5	14	from	productions	759:769	arg1	lymphocytes					830:840	the peripheral lymphocytes	815:840	the peripheral lymphocytes	815:840	They all could cause the proliferation of the lymphocyte, up-regulate stimulate the productions of IFN-γ, IL-2, IL-6 and TNF-α secretion in the peripheral lymphocytes.
26876821	1	15	theme	total	181:185	arg1	CAPSt					204:208	CAPSt	204:208	CAPSt	204:208	Four fractional polysaccharides (CAPS30, CAPS50, CAPS70 and CAPS80) and total polysaccharides (CAPSt) were obtained from Angelica sinensis.
26876821	1	15	theme	total	181:185	arg1	polysaccharides					187:201	total polysaccharides	181:201	total polysaccharides (CAPSt)	181:209	Four fractional polysaccharides (CAPS30, CAPS50, CAPS70 and CAPS80) and total polysaccharides (CAPSt) were obtained from Angelica sinensis.
26876821	5	16	theme	peripheral	819:828	arg1	lymphocytes					830:840	the peripheral lymphocytes	815:840	the peripheral lymphocytes	815:840	They all could cause the proliferation of the lymphocyte, up-regulate stimulate the productions of IFN-γ, IL-2, IL-6 and TNF-α secretion in the peripheral lymphocytes.
26876821	5	17	theme	IL-6	787:790	arg1	productions					759:769	the productions	755:769	the productions of IFN-γ, IL-2, IL-6 and TNF-α secretion in the peripheral lymphocytes	755:840	They all could cause the proliferation of the lymphocyte, up-regulate stimulate the productions of IFN-γ, IL-2, IL-6 and TNF-α secretion in the peripheral lymphocytes.
26876821	0	18	from	comparison	50:59	arg1	sinensis					99:106	Angelica sinensis	90:106	Angelica sinensis	90:106	Structural analysis and immunoregulation activity comparison of five polysaccharides from Angelica sinensis.
26876821	2	19	theme	gas	459:461	arg1	spectrometry					483:494	gas chromatography-mass spectrometry	459:494	gas chromatography-mass spectrometry (GC-MS)	459:502	Their structures were identified by Fourier transform-infrared spectroscopy (FT-IR), molecular weights were evaluated by high performance gel permeation chromatography (HPGPC) and compositions were analyzed by gas chromatography-mass spectrometry (GC-MS).
26876821	2	19	theme	gas	459:461	arg1	GC-MS					497:501	GC-MS	497:501	GC-MS	497:501	Their structures were identified by Fourier transform-infrared spectroscopy (FT-IR), molecular weights were evaluated by high performance gel permeation chromatography (HPGPC) and compositions were analyzed by gas chromatography-mass spectrometry (GC-MS).
26876821	6	20	theme	CD3	921:923	arg1	cells					935:939	CD3(+)CD56(+) cells	921:939	CD3(+)CD56(+) cells	921:939	Further experiments showed that CAPS50 and CAPS70 could increase the ratio of CD3(+)CD56(+) cells to some extent.
26876821	7	21	theme	strongest	1226:1234	arg1	activity					1255:1262	the strongest immuno-enhancement activity	1222:1262	the strongest immuno-enhancement activity	1222:1262	These indicated that five CAPSs displayed different activities which were associated with their different structural characteristics and CAPS70, with the molecular weights of 20.82kDa and consisting of mannose and glucose in the molar ratio of 1.20:1.01, possessed the strongest immuno-enhancement activity.
26876821	7	22	theme	molar	1186:1190	arg1	ratio					1192:1196	the molar ratio	1182:1196	the molar ratio of 1.20:1.01	1182:1209	These indicated that five CAPSs displayed different activities which were associated with their different structural characteristics and CAPS70, with the molecular weights of 20.82kDa and consisting of mannose and glucose in the molar ratio of 1.20:1.01, possessed the strongest immuno-enhancement activity.
26876821	2	23	theme	permeation	391:400	arg1	chromatography					402:415	high performance gel permeation chromatography	370:415	high performance gel permeation chromatography (HPGPC)	370:423	Their structures were identified by Fourier transform-infrared spectroscopy (FT-IR), molecular weights were evaluated by high performance gel permeation chromatography (HPGPC) and compositions were analyzed by gas chromatography-mass spectrometry (GC-MS).
26876821	2	23	theme	permeation	391:400	arg1	HPGPC					418:422	HPGPC	418:422	HPGPC	418:422	Their structures were identified by Fourier transform-infrared spectroscopy (FT-IR), molecular weights were evaluated by high performance gel permeation chromatography (HPGPC) and compositions were analyzed by gas chromatography-mass spectrometry (GC-MS).
26876821	0	24	theme	Structural	0:9	arg1	analysis					11:18	Structural analysis	0:18	Structural analysis	0:18	Structural analysis and immunoregulation activity comparison of five polysaccharides from Angelica sinensis.
26876821	2	25	theme	gel	387:389	arg1	chromatography					402:415	high performance gel permeation chromatography	370:415	high performance gel permeation chromatography (HPGPC)	370:423	Their structures were identified by Fourier transform-infrared spectroscopy (FT-IR), molecular weights were evaluated by high performance gel permeation chromatography (HPGPC) and compositions were analyzed by gas chromatography-mass spectrometry (GC-MS).
26876821	2	25	theme	gel	387:389	arg1	HPGPC					418:422	HPGPC	418:422	HPGPC	418:422	Their structures were identified by Fourier transform-infrared spectroscopy (FT-IR), molecular weights were evaluated by high performance gel permeation chromatography (HPGPC) and compositions were analyzed by gas chromatography-mass spectrometry (GC-MS).
26876821	0	26	theme	immunoregulation	24:39	arg1	comparison					50:59	immunoregulation activity comparison	24:59	immunoregulation activity comparison	24:59	Structural analysis and immunoregulation activity comparison of five polysaccharides from Angelica sinensis.
26876821	5	27	dep	cause	690:694	arg1	up-regulate					733:743	up-regulate	733:743	up-regulate stimulate the productions of IFN-γ, IL-2, IL-6 and TNF-α secretion in the peripheral lymphocytes	733:840	They all could cause the proliferation of the lymphocyte, up-regulate stimulate the productions of IFN-γ, IL-2, IL-6 and TNF-α secretion in the peripheral lymphocytes.
26876821	1	28	theme	fractional	114:123	arg1	CAPS70					158:163	CAPS70	158:163	CAPS70	158:163	Four fractional polysaccharides (CAPS30, CAPS50, CAPS70 and CAPS80) and total polysaccharides (CAPSt) were obtained from Angelica sinensis.
26876821	1	28	theme	fractional	114:123	arg1	CAPS80					169:174	CAPS80	169:174	CAPS80	169:174	Four fractional polysaccharides (CAPS30, CAPS50, CAPS70 and CAPS80) and total polysaccharides (CAPSt) were obtained from Angelica sinensis.
26876821	1	28	theme	fractional	114:123	arg1	polysaccharides					125:139	Four fractional polysaccharides	109:139	Four fractional polysaccharides (CAPS30, CAPS50, CAPS70 and CAPS80)	109:175	Four fractional polysaccharides (CAPS30, CAPS50, CAPS70 and CAPS80) and total polysaccharides (CAPSt) were obtained from Angelica sinensis.
26876821	0	29	from	sinensis	99:106	arg1	comparison					50:59	immunoregulation activity comparison	24:59	immunoregulation activity comparison	24:59	Structural analysis and immunoregulation activity comparison of five polysaccharides from Angelica sinensis.
26876821	0	29	from	sinensis	99:106	arg1	polysaccharides					69:83	five polysaccharides	64:83	five polysaccharides from Angelica sinensis	64:106	Structural analysis and immunoregulation activity comparison of five polysaccharides from Angelica sinensis.
26876821	0	29	from	sinensis	99:106	arg1	analysis					11:18	Structural analysis	0:18	Structural analysis	0:18	Structural analysis and immunoregulation activity comparison of five polysaccharides from Angelica sinensis.
26876821	7	30	dep	displayed	989:997	arg1	possessed					1212:1220	possessed	1212:1220	possessed the strongest immuno-enhancement activity	1212:1262	These indicated that five CAPSs displayed different activities which were associated with their different structural characteristics and CAPS70, with the molecular weights of 20.82kDa and consisting of mannose and glucose in the molar ratio of 1.20:1.01, possessed the strongest immuno-enhancement activity.
26876821	0	31	theme	activity	41:48	arg1	comparison					50:59	immunoregulation activity comparison	24:59	immunoregulation activity comparison	24:59	Structural analysis and immunoregulation activity comparison of five polysaccharides from Angelica sinensis.
26876821	5	32	theme	IFN-γ	774:778	arg1	productions					759:769	the productions	755:769	the productions of IFN-γ, IL-2, IL-6 and TNF-α secretion in the peripheral lymphocytes	755:840	They all could cause the proliferation of the lymphocyte, up-regulate stimulate the productions of IFN-γ, IL-2, IL-6 and TNF-α secretion in the peripheral lymphocytes.
26876821	7	33	theme	1.20:1.01	1201:1209	arg1	ratio					1192:1196	the molar ratio	1182:1196	the molar ratio of 1.20:1.01	1182:1209	These indicated that five CAPSs displayed different activities which were associated with their different structural characteristics and CAPS70, with the molecular weights of 20.82kDa and consisting of mannose and glucose in the molar ratio of 1.20:1.01, possessed the strongest immuno-enhancement activity.
26876821	5	34	theme	lymphocyte	721:730	arg1	proliferation					700:712	the proliferation	696:712	the proliferation of the lymphocyte	696:730	They all could cause the proliferation of the lymphocyte, up-regulate stimulate the productions of IFN-γ, IL-2, IL-6 and TNF-α secretion in the peripheral lymphocytes.
26876821	5	35	theme	TNF-α	796:800	arg1	secretion					802:810	TNF-α secretion	796:810	TNF-α secretion	796:810	They all could cause the proliferation of the lymphocyte, up-regulate stimulate the productions of IFN-γ, IL-2, IL-6 and TNF-α secretion in the peripheral lymphocytes.
26876821	2	36	theme	chromatography-mass	463:481	arg1	spectrometry					483:494	gas chromatography-mass spectrometry	459:494	gas chromatography-mass spectrometry (GC-MS)	459:502	Their structures were identified by Fourier transform-infrared spectroscopy (FT-IR), molecular weights were evaluated by high performance gel permeation chromatography (HPGPC) and compositions were analyzed by gas chromatography-mass spectrometry (GC-MS).
26876821	2	36	theme	chromatography-mass	463:481	arg1	GC-MS					497:501	GC-MS	497:501	GC-MS	497:501	Their structures were identified by Fourier transform-infrared spectroscopy (FT-IR), molecular weights were evaluated by high performance gel permeation chromatography (HPGPC) and compositions were analyzed by gas chromatography-mass spectrometry (GC-MS).
26876821	7	37	theme	structural	1063:1072	arg1	characteristics					1074:1088	different structural characteristics	1053:1088	different structural characteristics	1053:1088	These indicated that five CAPSs displayed different activities which were associated with their different structural characteristics and CAPS70, with the molecular weights of 20.82kDa and consisting of mannose and glucose in the molar ratio of 1.20:1.01, possessed the strongest immuno-enhancement activity.
26876821	3	38	theme	immunoregulation	511:526	arg1	activities					528:537	Their immunoregulation activities	505:537	Their immunoregulation activities	505:537	Their immunoregulation activities were further compared in vitro.
26876821	6	39	theme	cells	935:939	arg1	ratio					912:916	the ratio	908:916	the ratio of CD3(+)CD56(+) cells to some extent	908:954	Further experiments showed that CAPS50 and CAPS70 could increase the ratio of CD3(+)CD56(+) cells to some extent.
26876821	2	40	theme	molecular	334:342	arg1	weights					344:350	molecular weights	334:350	molecular weights	334:350	Their structures were identified by Fourier transform-infrared spectroscopy (FT-IR), molecular weights were evaluated by high performance gel permeation chromatography (HPGPC) and compositions were analyzed by gas chromatography-mass spectrometry (GC-MS).
26876821	4	41	theme	immuno-enhancement	644:661	arg1	activities					663:672	immuno-enhancement activities	644:672	immuno-enhancement activities	644:672	The results showed that they displayed different structural features and immuno-enhancement activities.
26876821	1	42	dep	polysaccharides	125:139	arg1	CAPS70					158:163	CAPS70	158:163	CAPS70	158:163	Four fractional polysaccharides (CAPS30, CAPS50, CAPS70 and CAPS80) and total polysaccharides (CAPSt) were obtained from Angelica sinensis.
26876821	1	42	dep	polysaccharides	125:139	arg1	polysaccharides					125:139	Four fractional polysaccharides	109:139	Four fractional polysaccharides (CAPS30, CAPS50, CAPS70 and CAPS80)	109:175	Four fractional polysaccharides (CAPS30, CAPS50, CAPS70 and CAPS80) and total polysaccharides (CAPSt) were obtained from Angelica sinensis.
26876821	1	42	dep	polysaccharides	125:139	arg1	CAPS80					169:174	CAPS80	169:174	CAPS80	169:174	Four fractional polysaccharides (CAPS30, CAPS50, CAPS70 and CAPS80) and total polysaccharides (CAPSt) were obtained from Angelica sinensis.
26876821	7	43	theme	immuno-enhancement	1236:1253	arg1	activity					1255:1262	the strongest immuno-enhancement activity	1222:1262	the strongest immuno-enhancement activity	1222:1262	These indicated that five CAPSs displayed different activities which were associated with their different structural characteristics and CAPS70, with the molecular weights of 20.82kDa and consisting of mannose and glucose in the molar ratio of 1.20:1.01, possessed the strongest immuno-enhancement activity.
26876821	7	44	theme	different	999:1007	arg1	activities					1009:1018	different activities	999:1018	different activities which were associated with their different structural characteristics and CAPS70, with the molecular weights of 20.82kDa and consisting of mannose and glucose	999:1177	These indicated that five CAPSs displayed different activities which were associated with their different structural characteristics and CAPS70, with the molecular weights of 20.82kDa and consisting of mannose and glucose in the molar ratio of 1.20:1.01, possessed the strongest immuno-enhancement activity.
26876821	2	45	theme	performance	375:385	arg1	chromatography					402:415	high performance gel permeation chromatography	370:415	high performance gel permeation chromatography (HPGPC)	370:423	Their structures were identified by Fourier transform-infrared spectroscopy (FT-IR), molecular weights were evaluated by high performance gel permeation chromatography (HPGPC) and compositions were analyzed by gas chromatography-mass spectrometry (GC-MS).
26876821	2	45	theme	performance	375:385	arg1	HPGPC					418:422	HPGPC	418:422	HPGPC	418:422	Their structures were identified by Fourier transform-infrared spectroscopy (FT-IR), molecular weights were evaluated by high performance gel permeation chromatography (HPGPC) and compositions were analyzed by gas chromatography-mass spectrometry (GC-MS).
26876821	1	46	theme	Angelica	230:237	arg1	sinensis					239:246	Angelica sinensis	230:246	Angelica sinensis	230:246	Four fractional polysaccharides (CAPS30, CAPS50, CAPS70 and CAPS80) and total polysaccharides (CAPSt) were obtained from Angelica sinensis.
26876821	0	47	theme	polysaccharides	69:83	arg1	comparison					50:59	immunoregulation activity comparison	24:59	immunoregulation activity comparison	24:59	Structural analysis and immunoregulation activity comparison of five polysaccharides from Angelica sinensis.
26876821	0	47	theme	polysaccharides	69:83	arg1	analysis					11:18	Structural analysis	0:18	Structural analysis	0:18	Structural analysis and immunoregulation activity comparison of five polysaccharides from Angelica sinensis.
26876821	2	48	theme	high	370:373	arg1	chromatography					402:415	high performance gel permeation chromatography	370:415	high performance gel permeation chromatography (HPGPC)	370:423	Their structures were identified by Fourier transform-infrared spectroscopy (FT-IR), molecular weights were evaluated by high performance gel permeation chromatography (HPGPC) and compositions were analyzed by gas chromatography-mass spectrometry (GC-MS).
26876821	2	48	theme	high	370:373	arg1	HPGPC					418:422	HPGPC	418:422	HPGPC	418:422	Their structures were identified by Fourier transform-infrared spectroscopy (FT-IR), molecular weights were evaluated by high performance gel permeation chromatography (HPGPC) and compositions were analyzed by gas chromatography-mass spectrometry (GC-MS).
27302279	8	0	theme	clinicopathological	1366:1384	arg1	factors					1386:1392	existing clinicopathological factors	1357:1392	existing clinicopathological factors	1357:1392	In the absence of validated biomarkers to improve upon prognostic information from existing clinicopathological factors, the potential of these novel IgG glycan biomarkers merits further investigation.
27302279	6	1	with	patients	1000:1007	arg1	time					1037:1040	the shortest follow-up time	1014:1040	the shortest follow-up time until death	1014:1052	The inclusion of IgG glycan data did not lead to any statistically significant improvements overall, but it improved the prediction over clinical models for stage 4 patients with the shortest follow-up time until death, with the median gain in the test AUC of 0.08.
27302279	4	2	theme	glycan	495:500	arg1	structures					502:511	fucosylated IgG glycan structures	479:511	fucosylated IgG glycan structures	479:511	Decreased galactosylation, decreased sialylation (of fucosylated IgG glycan structures) and increased bisecting GlcNAc in IgG glycan structures were strongly associated with all-cause (q < 0.01) and CRC mortality (q = 0.04 for galactosylation and sialylation).
27302279	1	3	theme	glycosylation	135:147	arg1	value					103:107	the potential value	89:107	the potential value of Immunoglobulin G (IgG) glycosylation	89:147	In this study we demonstrate the potential value of Immunoglobulin G (IgG) glycosylation as a novel prognostic biomarker of colorectal cancer (CRC).
27302279	1	3	theme	glycosylation	135:147	arg1	biomarker					171:179	a novel prognostic biomarker	152:179	a novel prognostic biomarker of colorectal cancer (CRC)	152:206	In this study we demonstrate the potential value of Immunoglobulin G (IgG) glycosylation as a novel prognostic biomarker of colorectal cancer (CRC).
27302279	8	4	theme	IgG	1424:1426	arg1	biomarkers					1435:1444	these novel IgG glycan biomarkers	1412:1444	these novel IgG glycan biomarkers	1412:1444	In the absence of validated biomarkers to improve upon prognostic information from existing clinicopathological factors, the potential of these novel IgG glycan biomarkers merits further investigation.
27302279	4	5	theme	glycan	552:557	arg1	structures					559:568	IgG glycan structures	548:568	IgG glycan structures	548:568	Decreased galactosylation, decreased sialylation (of fucosylated IgG glycan structures) and increased bisecting GlcNAc in IgG glycan structures were strongly associated with all-cause (q < 0.01) and CRC mortality (q = 0.04 for galactosylation and sialylation).
27302279	3	6	theme	predictive	316:325	arg1	value					327:331	the predictive value	312:331	the predictive value of clinical algorithms	312:354	We assessed the predictive value of clinical algorithms and compared this to algorithms that also included glycan predictors.
27302279	0	7	from	Glycosylation	0:12	arg1	prognosis					49:57	colorectal cancer prognosis	31:57	colorectal cancer prognosis	31:57	Glycosylation of plasma IgG in colorectal cancer prognosis.
27302279	7	8	from	consistent	1130:1139	arg1	CRC					1269:1271	late stage CRC	1258:1271	late stage CRC	1258:1271	These glycan differences are consistent with significantly increased IgG pro-inflammatory activity being associated with poorer CRC prognosis, especially in late stage CRC.
27302279	2	9	theme	CRC	248:250	arg1	patients					252:259	1229 CRC patients	243:259	1229 CRC patients	243:259	We analysed plasma IgG glycans in 1229 CRC patients and correlated with survival outcomes.
27302279	8	10	theme	prognostic	1329:1338	arg1	information					1340:1350	prognostic information	1329:1350	prognostic information from existing clinicopathological factors	1329:1392	In the absence of validated biomarkers to improve upon prognostic information from existing clinicopathological factors, the potential of these novel IgG glycan biomarkers merits further investigation.
27302279	7	11	theme	increased	1160:1168	arg1	activity					1191:1198	significantly increased IgG pro-inflammatory activity	1146:1198	significantly increased IgG pro-inflammatory activity	1146:1198	These glycan differences are consistent with significantly increased IgG pro-inflammatory activity being associated with poorer CRC prognosis, especially in late stage CRC.
27302279	4	12	theme	decreased	453:461	arg1	sialylation					463:473	decreased sialylation	453:473	decreased sialylation (of fucosylated IgG glycan structures)	453:512	Decreased galactosylation, decreased sialylation (of fucosylated IgG glycan structures) and increased bisecting GlcNAc in IgG glycan structures were strongly associated with all-cause (q < 0.01) and CRC mortality (q = 0.04 for galactosylation and sialylation).
27302279	6	13	theme	IgG	852:854	arg1	data					863:866	IgG glycan data	852:866	IgG glycan data	852:866	The inclusion of IgG glycan data did not lead to any statistically significant improvements overall, but it improved the prediction over clinical models for stage 4 patients with the shortest follow-up time until death, with the median gain in the test AUC of 0.08.
27302279	6	14	from	gain	1071:1074	arg1	AUC					1088:1090	the test AUC	1079:1090	the test AUC of 0.08	1079:1098	The inclusion of IgG glycan data did not lead to any statistically significant improvements overall, but it improved the prediction over clinical models for stage 4 patients with the shortest follow-up time until death, with the median gain in the test AUC of 0.08.
27302279	2	15	theme	survival	281:288	arg1	outcomes					290:297	survival outcomes	281:297	survival outcomes	281:297	We analysed plasma IgG glycans in 1229 CRC patients and correlated with survival outcomes.
27302279	8	16	theme	biomarkers	1435:1444	arg1	potential					1399:1407	the potential	1395:1407	the potential of these novel IgG glycan biomarkers	1395:1444	In the absence of validated biomarkers to improve upon prognostic information from existing clinicopathological factors, the potential of these novel IgG glycan biomarkers merits further investigation.
27302279	5	17	dep	C	772:772	arg1	AUC					787:789	AUC	787:789	AUC: 0.75	787:795	Clinical algorithms showed good prediction of all-cause and CRC mortality (Harrell's C: 0.73, 0.77; AUC: 0.75, 0.79, IDI: 0.02, 0.04 respectively).
27302279	5	17	dep	C	772:772	arg1	0.02					809:812	0.02	809:812	0.02	809:812	Clinical algorithms showed good prediction of all-cause and CRC mortality (Harrell's C: 0.73, 0.77; AUC: 0.75, 0.79, IDI: 0.02, 0.04 respectively).
27302279	5	17	dep	C	772:772	arg1	0.73					775:778	0.73	775:778	0.73	775:778	Clinical algorithms showed good prediction of all-cause and CRC mortality (Harrell's C: 0.73, 0.77; AUC: 0.75, 0.79, IDI: 0.02, 0.04 respectively).
27302279	5	17	dep	C	772:772	arg1	0.04					815:818	0.04	815:818	0.04	815:818	Clinical algorithms showed good prediction of all-cause and CRC mortality (Harrell's C: 0.73, 0.77; AUC: 0.75, 0.79, IDI: 0.02, 0.04 respectively).
27302279	5	17	dep	C	772:772	arg1	0.77					781:784	0.77	781:784	0.77	781:784	Clinical algorithms showed good prediction of all-cause and CRC mortality (Harrell's C: 0.73, 0.77; AUC: 0.75, 0.79, IDI: 0.02, 0.04 respectively).
27302279	5	17	dep	C	772:772	arg1	0.79					798:801	0.79	798:801	0.79	798:801	Clinical algorithms showed good prediction of all-cause and CRC mortality (Harrell's C: 0.73, 0.77; AUC: 0.75, 0.79, IDI: 0.02, 0.04 respectively).
27302279	5	17	dep	C	772:772	arg1	IDI					804:806	IDI	804:806	IDI	804:806	Clinical algorithms showed good prediction of all-cause and CRC mortality (Harrell's C: 0.73, 0.77; AUC: 0.75, 0.79, IDI: 0.02, 0.04 respectively).
27302279	4	18	gly	fucosylated	479:489	arg1	structures					502:511	fucosylated IgG glycan structures	479:511	fucosylated IgG glycan structures	479:511	Decreased galactosylation, decreased sialylation (of fucosylated IgG glycan structures) and increased bisecting GlcNAc in IgG glycan structures were strongly associated with all-cause (q < 0.01) and CRC mortality (q = 0.04 for galactosylation and sialylation).
27302279	6	19	theme	stage	992:996	arg1	patients					1000:1007	stage 4 patients	992:1007	stage 4 patients with the shortest follow-up time until death	992:1052	The inclusion of IgG glycan data did not lead to any statistically significant improvements overall, but it improved the prediction over clinical models for stage 4 patients with the shortest follow-up time until death, with the median gain in the test AUC of 0.08.
27302279	5	20	theme	all-cause	733:741	arg1	prediction					719:728	good prediction	714:728	good prediction of all-cause and CRC mortality	714:759	Clinical algorithms showed good prediction of all-cause and CRC mortality (Harrell's C: 0.73, 0.77; AUC: 0.75, 0.79, IDI: 0.02, 0.04 respectively).
27302279	4	21	from	sialylation	463:473	arg1	structures					559:568	IgG glycan structures	548:568	IgG glycan structures	548:568	Decreased galactosylation, decreased sialylation (of fucosylated IgG glycan structures) and increased bisecting GlcNAc in IgG glycan structures were strongly associated with all-cause (q < 0.01) and CRC mortality (q = 0.04 for galactosylation and sialylation).
27302279	7	22	from	CRC	1269:1271	arg1	consistent					1130:1139	consistent	1130:1139	consistent	1130:1139	These glycan differences are consistent with significantly increased IgG pro-inflammatory activity being associated with poorer CRC prognosis, especially in late stage CRC.
27302279	6	23	theme	median	1064:1069	arg1	gain					1071:1074	the median gain	1060:1074	the median gain in the test AUC of 0.08	1060:1098	The inclusion of IgG glycan data did not lead to any statistically significant improvements overall, but it improved the prediction over clinical models for stage 4 patients with the shortest follow-up time until death, with the median gain in the test AUC of 0.08.
27302279	8	24	theme	validated	1292:1300	arg1	biomarkers					1302:1311	validated biomarkers	1292:1311	validated biomarkers	1292:1311	In the absence of validated biomarkers to improve upon prognostic information from existing clinicopathological factors, the potential of these novel IgG glycan biomarkers merits further investigation.
27302279	3	25	theme	glycan	407:412	arg1	predictors					414:423	glycan predictors	407:423	glycan predictors	407:423	We assessed the predictive value of clinical algorithms and compared this to algorithms that also included glycan predictors.
27302279	3	26	theme	algorithms	345:354	arg1	value					327:331	the predictive value	312:331	the predictive value of clinical algorithms	312:354	We assessed the predictive value of clinical algorithms and compared this to algorithms that also included glycan predictors.
27302279	8	27	theme	further	1453:1459	arg1	investigation					1461:1473	further investigation	1453:1473	further investigation	1453:1473	In the absence of validated biomarkers to improve upon prognostic information from existing clinicopathological factors, the potential of these novel IgG glycan biomarkers merits further investigation.
27302279	8	28	from	factors	1386:1392	arg1	information					1340:1350	prognostic information	1329:1350	prognostic information from existing clinicopathological factors	1329:1392	In the absence of validated biomarkers to improve upon prognostic information from existing clinicopathological factors, the potential of these novel IgG glycan biomarkers merits further investigation.
27302279	7	29	theme	IgG	1170:1172	arg1	activity					1191:1198	significantly increased IgG pro-inflammatory activity	1146:1198	significantly increased IgG pro-inflammatory activity	1146:1198	These glycan differences are consistent with significantly increased IgG pro-inflammatory activity being associated with poorer CRC prognosis, especially in late stage CRC.
27302279	1	30	theme	novel	154:158	arg1	value					103:107	the potential value	89:107	the potential value of Immunoglobulin G (IgG) glycosylation	89:147	In this study we demonstrate the potential value of Immunoglobulin G (IgG) glycosylation as a novel prognostic biomarker of colorectal cancer (CRC).
27302279	1	30	theme	novel	154:158	arg1	biomarker					171:179	a novel prognostic biomarker	152:179	a novel prognostic biomarker of colorectal cancer (CRC)	152:206	In this study we demonstrate the potential value of Immunoglobulin G (IgG) glycosylation as a novel prognostic biomarker of colorectal cancer (CRC).
27302279	8	31	theme	glycan	1428:1433	arg1	biomarkers					1435:1444	these novel IgG glycan biomarkers	1412:1444	these novel IgG glycan biomarkers	1412:1444	In the absence of validated biomarkers to improve upon prognostic information from existing clinicopathological factors, the potential of these novel IgG glycan biomarkers merits further investigation.
27302279	8	32	from	merits	1446:1451	arg1	absence					1281:1287	the absence	1277:1287	the absence of validated biomarkers to improve upon prognostic information from existing clinicopathological factors	1277:1392	In the absence of validated biomarkers to improve upon prognostic information from existing clinicopathological factors, the potential of these novel IgG glycan biomarkers merits further investigation.
27302279	7	33	theme	stage	1263:1267	arg1	CRC					1269:1271	late stage CRC	1258:1271	late stage CRC	1258:1271	These glycan differences are consistent with significantly increased IgG pro-inflammatory activity being associated with poorer CRC prognosis, especially in late stage CRC.
27302279	5	34	theme	good	714:717	arg1	prediction					719:728	good prediction	714:728	good prediction of all-cause and CRC mortality	714:759	Clinical algorithms showed good prediction of all-cause and CRC mortality (Harrell's C: 0.73, 0.77; AUC: 0.75, 0.79, IDI: 0.02, 0.04 respectively).
27302279	1	35	theme	prognostic	160:169	arg1	value					103:107	the potential value	89:107	the potential value of Immunoglobulin G (IgG) glycosylation	89:147	In this study we demonstrate the potential value of Immunoglobulin G (IgG) glycosylation as a novel prognostic biomarker of colorectal cancer (CRC).
27302279	1	35	theme	prognostic	160:169	arg1	biomarker					171:179	a novel prognostic biomarker	152:179	a novel prognostic biomarker of colorectal cancer (CRC)	152:206	In this study we demonstrate the potential value of Immunoglobulin G (IgG) glycosylation as a novel prognostic biomarker of colorectal cancer (CRC).
27302279	0	36	theme	IgG	24:26	arg1	Glycosylation					0:12	Glycosylation	0:12	Glycosylation of plasma IgG in colorectal cancer prognosis.	0:58	Glycosylation of plasma IgG in colorectal cancer prognosis.
27302279	4	37	theme	bisecting	528:536	arg1	GlcNAc					538:543	increased bisecting GlcNAc	518:543	increased bisecting GlcNAc in IgG glycan structures	518:568	Decreased galactosylation, decreased sialylation (of fucosylated IgG glycan structures) and increased bisecting GlcNAc in IgG glycan structures were strongly associated with all-cause (q < 0.01) and CRC mortality (q = 0.04 for galactosylation and sialylation).
27302279	4	38	from	galactosylation	436:450	arg1	structures					559:568	IgG glycan structures	548:568	IgG glycan structures	548:568	Decreased galactosylation, decreased sialylation (of fucosylated IgG glycan structures) and increased bisecting GlcNAc in IgG glycan structures were strongly associated with all-cause (q < 0.01) and CRC mortality (q = 0.04 for galactosylation and sialylation).
27302279	7	39	theme	late	1258:1261	arg1	CRC					1269:1271	late stage CRC	1258:1271	late stage CRC	1258:1271	These glycan differences are consistent with significantly increased IgG pro-inflammatory activity being associated with poorer CRC prognosis, especially in late stage CRC.
27302279	8	40	theme	novel	1418:1422	arg1	biomarkers					1435:1444	these novel IgG glycan biomarkers	1412:1444	these novel IgG glycan biomarkers	1412:1444	In the absence of validated biomarkers to improve upon prognostic information from existing clinicopathological factors, the potential of these novel IgG glycan biomarkers merits further investigation.
27302279	3	41	theme	clinical	336:343	arg1	algorithms					345:354	clinical algorithms	336:354	clinical algorithms	336:354	We assessed the predictive value of clinical algorithms and compared this to algorithms that also included glycan predictors.
27302279	4	42	theme	IgG	548:550	arg1	structures					559:568	IgG glycan structures	548:568	IgG glycan structures	548:568	Decreased galactosylation, decreased sialylation (of fucosylated IgG glycan structures) and increased bisecting GlcNAc in IgG glycan structures were strongly associated with all-cause (q < 0.01) and CRC mortality (q = 0.04 for galactosylation and sialylation).
27302279	5	43	theme	Clinical	687:694	arg1	algorithms					696:705	Clinical algorithms	687:705	Clinical algorithms	687:705	Clinical algorithms showed good prediction of all-cause and CRC mortality (Harrell's C: 0.73, 0.77; AUC: 0.75, 0.79, IDI: 0.02, 0.04 respectively).
27302279	8	44	theme	existing	1357:1364	arg1	factors					1386:1392	existing clinicopathological factors	1357:1392	existing clinicopathological factors	1357:1392	In the absence of validated biomarkers to improve upon prognostic information from existing clinicopathological factors, the potential of these novel IgG glycan biomarkers merits further investigation.
27302279	1	45	theme	potential	93:101	arg1	value					103:107	the potential value	89:107	the potential value of Immunoglobulin G (IgG) glycosylation	89:147	In this study we demonstrate the potential value of Immunoglobulin G (IgG) glycosylation as a novel prognostic biomarker of colorectal cancer (CRC).
27302279	1	45	theme	potential	93:101	arg1	biomarker					171:179	a novel prognostic biomarker	152:179	a novel prognostic biomarker of colorectal cancer (CRC)	152:206	In this study we demonstrate the potential value of Immunoglobulin G (IgG) glycosylation as a novel prognostic biomarker of colorectal cancer (CRC).
27302279	4	46	gly	sialylation	463:473	arg1	structures					502:511	fucosylated IgG glycan structures	479:511	fucosylated IgG glycan structures	479:511	Decreased galactosylation, decreased sialylation (of fucosylated IgG glycan structures) and increased bisecting GlcNAc in IgG glycan structures were strongly associated with all-cause (q < 0.01) and CRC mortality (q = 0.04 for galactosylation and sialylation).
27302279	4	46	gly	sialylation	463:473	arg1	structures					559:568	IgG glycan structures	548:568	IgG glycan structures	548:568	Decreased galactosylation, decreased sialylation (of fucosylated IgG glycan structures) and increased bisecting GlcNAc in IgG glycan structures were strongly associated with all-cause (q < 0.01) and CRC mortality (q = 0.04 for galactosylation and sialylation).
27302279	7	47	theme	poorer	1222:1227	arg1	prognosis					1233:1241	poorer CRC prognosis	1222:1241	poorer CRC prognosis	1222:1241	These glycan differences are consistent with significantly increased IgG pro-inflammatory activity being associated with poorer CRC prognosis, especially in late stage CRC.
27302279	0	48	theme	colorectal	31:40	arg1	prognosis					49:57	colorectal cancer prognosis	31:57	colorectal cancer prognosis	31:57	Glycosylation of plasma IgG in colorectal cancer prognosis.
27302279	4	49	theme	CRC	625:627	arg1	mortality					629:637	CRC mortality	625:637	CRC mortality (q = 0.04 for galactosylation and sialylation)	625:684	Decreased galactosylation, decreased sialylation (of fucosylated IgG glycan structures) and increased bisecting GlcNAc in IgG glycan structures were strongly associated with all-cause (q < 0.01) and CRC mortality (q = 0.04 for galactosylation and sialylation).
27302279	0	50	gly	Glycosylation	0:12	arg1	IgG					24:26	plasma IgG	17:26	plasma IgG	17:26	Glycosylation of plasma IgG in colorectal cancer prognosis.
27302279	6	51	theme	test	1083:1086	arg1	AUC					1088:1090	the test AUC	1079:1090	the test AUC of 0.08	1079:1098	The inclusion of IgG glycan data did not lead to any statistically significant improvements overall, but it improved the prediction over clinical models for stage 4 patients with the shortest follow-up time until death, with the median gain in the test AUC of 0.08.
27302279	1	52	theme	colorectal	184:193	arg1	CRC					203:205	CRC	203:205	CRC	203:205	In this study we demonstrate the potential value of Immunoglobulin G (IgG) glycosylation as a novel prognostic biomarker of colorectal cancer (CRC).
27302279	1	52	theme	colorectal	184:193	arg1	cancer					195:200	colorectal cancer	184:200	colorectal cancer (CRC)	184:206	In this study we demonstrate the potential value of Immunoglobulin G (IgG) glycosylation as a novel prognostic biomarker of colorectal cancer (CRC).
27302279	6	53	theme	data	863:866	arg1	inclusion					839:847	The inclusion	835:847	The inclusion of IgG glycan data	835:866	The inclusion of IgG glycan data did not lead to any statistically significant improvements overall, but it improved the prediction over clinical models for stage 4 patients with the shortest follow-up time until death, with the median gain in the test AUC of 0.08.
27302279	6	54	theme	significant	902:912	arg1	improvements					914:925	any statistically significant improvements	884:925	any statistically significant improvements overall	884:933	The inclusion of IgG glycan data did not lead to any statistically significant improvements overall, but it improved the prediction over clinical models for stage 4 patients with the shortest follow-up time until death, with the median gain in the test AUC of 0.08.
27302279	4	55	from	GlcNAc	538:543	arg1	structures					559:568	IgG glycan structures	548:568	IgG glycan structures	548:568	Decreased galactosylation, decreased sialylation (of fucosylated IgG glycan structures) and increased bisecting GlcNAc in IgG glycan structures were strongly associated with all-cause (q < 0.01) and CRC mortality (q = 0.04 for galactosylation and sialylation).
27302279	1	56	theme	cancer	195:200	arg1	value					103:107	the potential value	89:107	the potential value of Immunoglobulin G (IgG) glycosylation	89:147	In this study we demonstrate the potential value of Immunoglobulin G (IgG) glycosylation as a novel prognostic biomarker of colorectal cancer (CRC).
27302279	1	56	theme	cancer	195:200	arg1	biomarker					171:179	a novel prognostic biomarker	152:179	a novel prognostic biomarker of colorectal cancer (CRC)	152:206	In this study we demonstrate the potential value of Immunoglobulin G (IgG) glycosylation as a novel prognostic biomarker of colorectal cancer (CRC).
27302279	6	57	theme	clinical	972:979	arg1	models					981:986	clinical models	972:986	clinical models for stage 4 patients with the shortest follow-up time until death	972:1052	The inclusion of IgG glycan data did not lead to any statistically significant improvements overall, but it improved the prediction over clinical models for stage 4 patients with the shortest follow-up time until death, with the median gain in the test AUC of 0.08.
27302279	4	58	theme	structures	502:511	arg1	sialylation					463:473	decreased sialylation	453:473	decreased sialylation (of fucosylated IgG glycan structures)	453:512	Decreased galactosylation, decreased sialylation (of fucosylated IgG glycan structures) and increased bisecting GlcNAc in IgG glycan structures were strongly associated with all-cause (q < 0.01) and CRC mortality (q = 0.04 for galactosylation and sialylation).
27302279	4	58	theme	structures	502:511	arg1	GlcNAc					538:543	increased bisecting GlcNAc	518:543	increased bisecting GlcNAc in IgG glycan structures	518:568	Decreased galactosylation, decreased sialylation (of fucosylated IgG glycan structures) and increased bisecting GlcNAc in IgG glycan structures were strongly associated with all-cause (q < 0.01) and CRC mortality (q = 0.04 for galactosylation and sialylation).
27302279	4	58	theme	structures	502:511	arg1	galactosylation					436:450	Decreased galactosylation	426:450	Decreased galactosylation	426:450	Decreased galactosylation, decreased sialylation (of fucosylated IgG glycan structures) and increased bisecting GlcNAc in IgG glycan structures were strongly associated with all-cause (q < 0.01) and CRC mortality (q = 0.04 for galactosylation and sialylation).
27302279	4	59	theme	Decreased	426:434	arg1	galactosylation					436:450	Decreased galactosylation	426:450	Decreased galactosylation	426:450	Decreased galactosylation, decreased sialylation (of fucosylated IgG glycan structures) and increased bisecting GlcNAc in IgG glycan structures were strongly associated with all-cause (q < 0.01) and CRC mortality (q = 0.04 for galactosylation and sialylation).
27302279	7	60	theme	pro-inflammatory	1174:1189	arg1	activity					1191:1198	significantly increased IgG pro-inflammatory activity	1146:1198	significantly increased IgG pro-inflammatory activity	1146:1198	These glycan differences are consistent with significantly increased IgG pro-inflammatory activity being associated with poorer CRC prognosis, especially in late stage CRC.
27302279	0	61	theme	cancer	42:47	arg1	prognosis					49:57	colorectal cancer prognosis	31:57	colorectal cancer prognosis	31:57	Glycosylation of plasma IgG in colorectal cancer prognosis.
27302279	4	62	theme	increased	518:526	arg1	GlcNAc					538:543	increased bisecting GlcNAc	518:543	increased bisecting GlcNAc in IgG glycan structures	518:568	Decreased galactosylation, decreased sialylation (of fucosylated IgG glycan structures) and increased bisecting GlcNAc in IgG glycan structures were strongly associated with all-cause (q < 0.01) and CRC mortality (q = 0.04 for galactosylation and sialylation).
27302279	5	63	dep	all-cause	733:741	arg1	mortality					751:759	mortality	751:759	mortality	751:759	Clinical algorithms showed good prediction of all-cause and CRC mortality (Harrell's C: 0.73, 0.77; AUC: 0.75, 0.79, IDI: 0.02, 0.04 respectively).
27302279	8	64	theme	biomarkers	1302:1311	arg1	absence					1281:1287	the absence	1277:1287	the absence of validated biomarkers to improve upon prognostic information from existing clinicopathological factors	1277:1392	In the absence of validated biomarkers to improve upon prognostic information from existing clinicopathological factors, the potential of these novel IgG glycan biomarkers merits further investigation.
27302279	4	65	dep	mortality	629:637	arg1	q = 0.04					640:647	q = 0.04	640:647	q = 0.04	640:647	Decreased galactosylation, decreased sialylation (of fucosylated IgG glycan structures) and increased bisecting GlcNAc in IgG glycan structures were strongly associated with all-cause (q < 0.01) and CRC mortality (q = 0.04 for galactosylation and sialylation).
27302279	6	66	theme	0.08	1095:1098	arg1	AUC					1088:1090	the test AUC	1079:1090	the test AUC of 0.08	1079:1098	The inclusion of IgG glycan data did not lead to any statistically significant improvements overall, but it improved the prediction over clinical models for stage 4 patients with the shortest follow-up time until death, with the median gain in the test AUC of 0.08.
27302279	2	67	theme	IgG	228:230	arg1	glycans					232:238	plasma IgG glycans	221:238	plasma IgG glycans	221:238	We analysed plasma IgG glycans in 1229 CRC patients and correlated with survival outcomes.
27302279	1	68	theme	Immunoglobulin	112:125	arg1	IgG					130:132	IgG	130:132	IgG	130:132	In this study we demonstrate the potential value of Immunoglobulin G (IgG) glycosylation as a novel prognostic biomarker of colorectal cancer (CRC).
27302279	1	68	theme	Immunoglobulin	112:125	arg1	G					127:127	Immunoglobulin G	112:127	Immunoglobulin G (IgG) glycosylation	112:147	In this study we demonstrate the potential value of Immunoglobulin G (IgG) glycosylation as a novel prognostic biomarker of colorectal cancer (CRC).
27302279	6	69	theme	shortest	1018:1025	arg1	time					1037:1040	the shortest follow-up time	1014:1040	the shortest follow-up time until death	1014:1052	The inclusion of IgG glycan data did not lead to any statistically significant improvements overall, but it improved the prediction over clinical models for stage 4 patients with the shortest follow-up time until death, with the median gain in the test AUC of 0.08.
27302279	4	70	theme	IgG	491:493	arg1	structures					502:511	fucosylated IgG glycan structures	479:511	fucosylated IgG glycan structures	479:511	Decreased galactosylation, decreased sialylation (of fucosylated IgG glycan structures) and increased bisecting GlcNAc in IgG glycan structures were strongly associated with all-cause (q < 0.01) and CRC mortality (q = 0.04 for galactosylation and sialylation).
27302279	2	71	theme	plasma	221:226	arg1	glycans					232:238	plasma IgG glycans	221:238	plasma IgG glycans	221:238	We analysed plasma IgG glycans in 1229 CRC patients and correlated with survival outcomes.
27302279	1	72	theme	G	127:127	arg1	glycosylation					135:147	Immunoglobulin G (IgG) glycosylation	112:147	Immunoglobulin G (IgG) glycosylation	112:147	In this study we demonstrate the potential value of Immunoglobulin G (IgG) glycosylation as a novel prognostic biomarker of colorectal cancer (CRC).
27302279	7	73	theme	CRC	1229:1231	arg1	prognosis					1233:1241	poorer CRC prognosis	1222:1241	poorer CRC prognosis	1222:1241	These glycan differences are consistent with significantly increased IgG pro-inflammatory activity being associated with poorer CRC prognosis, especially in late stage CRC.
27302279	6	74	theme	follow-up	1027:1035	arg1	time					1037:1040	the shortest follow-up time	1014:1040	the shortest follow-up time until death	1014:1052	The inclusion of IgG glycan data did not lead to any statistically significant improvements overall, but it improved the prediction over clinical models for stage 4 patients with the shortest follow-up time until death, with the median gain in the test AUC of 0.08.
27302279	7	75	theme	glycan	1107:1112	arg1	differences					1114:1124	These glycan differences	1101:1124	These glycan differences	1101:1124	These glycan differences are consistent with significantly increased IgG pro-inflammatory activity being associated with poorer CRC prognosis, especially in late stage CRC.
27302279	5	76	dep	AUC	787:789	arg1	0.75					792:795	0.75	792:795	0.75	792:795	Clinical algorithms showed good prediction of all-cause and CRC mortality (Harrell's C: 0.73, 0.77; AUC: 0.75, 0.79, IDI: 0.02, 0.04 respectively).
27302279	5	77	theme	CRC	747:749	arg1	prediction					719:728	good prediction	714:728	good prediction of all-cause and CRC mortality	714:759	Clinical algorithms showed good prediction of all-cause and CRC mortality (Harrell's C: 0.73, 0.77; AUC: 0.75, 0.79, IDI: 0.02, 0.04 respectively).
27302279	4	78	theme	fucosylated	479:489	arg1	structures					502:511	fucosylated IgG glycan structures	479:511	fucosylated IgG glycan structures	479:511	Decreased galactosylation, decreased sialylation (of fucosylated IgG glycan structures) and increased bisecting GlcNAc in IgG glycan structures were strongly associated with all-cause (q < 0.01) and CRC mortality (q = 0.04 for galactosylation and sialylation).
27302279	0	79	theme	plasma	17:22	arg1	IgG					24:26	plasma IgG	17:26	plasma IgG	17:26	Glycosylation of plasma IgG in colorectal cancer prognosis.
27302279	6	80	theme	glycan	856:861	arg1	data					863:866	IgG glycan data	852:866	IgG glycan data	852:866	The inclusion of IgG glycan data did not lead to any statistically significant improvements overall, but it improved the prediction over clinical models for stage 4 patients with the shortest follow-up time until death, with the median gain in the test AUC of 0.08.
27302279	5	81	dep	showed	707:712	arg1	C					772:772	Harrell's C	762:772	Harrell's C	762:772	Clinical algorithms showed good prediction of all-cause and CRC mortality (Harrell's C: 0.73, 0.77; AUC: 0.75, 0.79, IDI: 0.02, 0.04 respectively).
25843830	2	0	theme	nuclear	500:506	arg1	resonance					517:525	nuclear magnetic resonance	500:525	nuclear magnetic resonance	500:525	The chemical composition and structural features of the hemicellulosic fractions obtained were comparatively characterized by the combination of high-performance anion-exchange chromatography, gel permeation chromatography, Fourier transform infrared, and nuclear magnetic resonance spectroscopies.
25843830	2	1	dep	transform	476:484	arg1	infrared					486:493	infrared	486:493	transform infrared	476:493	The chemical composition and structural features of the hemicellulosic fractions obtained were comparatively characterized by the combination of high-performance anion-exchange chromatography, gel permeation chromatography, Fourier transform infrared, and nuclear magnetic resonance spectroscopies.
25843830	1	2	theme	%	173:173	arg1	ethanol					175:181	70% ethanol	171:181	70% ethanol containing 0.4, 1.0, 2.0, 3.0, and 5.0% NaOH for 2h at 80°C	171:241	Eucalyptus was sequentially extracted with 70% ethanol containing 0.4, 1.0, 2.0, 3.0, and 5.0% NaOH for 2h at 80°C.
25843830	0	3	from	characterization	11:26	arg1	wall					90:93	Eucalyptus cell wall	74:93	Eucalyptus cell wall	74:93	Structural characterization of hemicelluloses and topochemical changes in Eucalyptus cell wall during alkali ethanol treatment.
25843830	2	4	dep	Fourier	468:474	arg1	transform					476:484	transform	476:484	transform infrared	476:493	The chemical composition and structural features of the hemicellulosic fractions obtained were comparatively characterized by the combination of high-performance anion-exchange chromatography, gel permeation chromatography, Fourier transform infrared, and nuclear magnetic resonance spectroscopies.
25843830	6	5	theme	chemical	1178:1185	arg1	characterization					1187:1202	chemical characterization	1178:1202	chemical characterization	1178:1202	The information obtained from the study conducted by combining chemical characterization with ultrastructure provides important basis for studying the mechanism of the alkali treatment.
25843830	4	6	dep	transform	688:696	arg1	infrared					698:705	infrared	698:705	transform infrared	688:705	Based on the Fourier transform infrared and nuclear magnetic resonance analyses, the hemicelluloses extracted from Eucalyptus mainly have a linear backbone of (1→4)-linked-β-d-xylopyranosyl residues decorated with branch at O-2 of 4-O-methyl-α-glucuronic acid unit.
25843830	4	7	theme	unit	927:930	arg1	O-2					891:893	O-2	891:893	O-2 of 4-O-methyl-α-glucuronic acid unit	891:930	Based on the Fourier transform infrared and nuclear magnetic resonance analyses, the hemicelluloses extracted from Eucalyptus mainly have a linear backbone of (1→4)-linked-β-d-xylopyranosyl residues decorated with branch at O-2 of 4-O-methyl-α-glucuronic acid unit.
25843830	0	8	theme	ethanol	109:115	arg1	treatment					117:125	alkali ethanol treatment	102:125	alkali ethanol treatment	102:125	Structural characterization of hemicelluloses and topochemical changes in Eucalyptus cell wall during alkali ethanol treatment.
25843830	4	9	theme	nuclear	711:717	arg1	resonance					728:736	nuclear magnetic resonance	711:736	nuclear magnetic resonance	711:736	Based on the Fourier transform infrared and nuclear magnetic resonance analyses, the hemicelluloses extracted from Eucalyptus mainly have a linear backbone of (1→4)-linked-β-d-xylopyranosyl residues decorated with branch at O-2 of 4-O-methyl-α-glucuronic acid unit.
25843830	2	10	theme	chemical	248:255	arg1	composition					257:267	chemical composition	248:267	chemical composition	248:267	The chemical composition and structural features of the hemicellulosic fractions obtained were comparatively characterized by the combination of high-performance anion-exchange chromatography, gel permeation chromatography, Fourier transform infrared, and nuclear magnetic resonance spectroscopies.
25843830	3	11	from	distribution	575:586	arg1	wall					614:617	cell wall	609:617	cell wall	609:617	Furthermore, the main component distribution and their changes in cell wall were investigated by confocal Raman microscopy.
25843830	0	12	theme	alkali	102:107	arg1	treatment					117:125	alkali ethanol treatment	102:125	alkali ethanol treatment	102:125	Structural characterization of hemicelluloses and topochemical changes in Eucalyptus cell wall during alkali ethanol treatment.
25843830	0	13	from	changes	63:69	arg1	wall					90:93	Eucalyptus cell wall	74:93	Eucalyptus cell wall	74:93	Structural characterization of hemicelluloses and topochemical changes in Eucalyptus cell wall during alkali ethanol treatment.
25843830	5	14	from	different	1000:1008	arg1	regions					1031:1037	the morphological regions	1013:1037	the morphological regions	1013:1037	Raman analysis revealed that the dissolution of hemicelluloses was different in the morphological regions, and the hemicelluloses released mainly originated from the secondary wall.
25843830	0	15	theme	changes	63:69	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization of hemicelluloses and topochemical changes in Eucalyptus cell wall during alkali ethanol treatment.	0:126	Structural characterization of hemicelluloses and topochemical changes in Eucalyptus cell wall during alkali ethanol treatment.
25843830	4	16	from	O-2	891:893	arg1	branch					881:886	branch	881:886	branch at O-2 of 4-O-methyl-α-glucuronic acid unit	881:930	Based on the Fourier transform infrared and nuclear magnetic resonance analyses, the hemicelluloses extracted from Eucalyptus mainly have a linear backbone of (1→4)-linked-β-d-xylopyranosyl residues decorated with branch at O-2 of 4-O-methyl-α-glucuronic acid unit.
25843830	0	17	theme	Structural	0:9	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization of hemicelluloses and topochemical changes in Eucalyptus cell wall during alkali ethanol treatment.	0:126	Structural characterization of hemicelluloses and topochemical changes in Eucalyptus cell wall during alkali ethanol treatment.
25843830	4	18	theme	4-O-methyl-α-glucuronic	898:920	arg1	unit					927:930	4-O-methyl-α-glucuronic acid unit	898:930	4-O-methyl-α-glucuronic acid unit	898:930	Based on the Fourier transform infrared and nuclear magnetic resonance analyses, the hemicelluloses extracted from Eucalyptus mainly have a linear backbone of (1→4)-linked-β-d-xylopyranosyl residues decorated with branch at O-2 of 4-O-methyl-α-glucuronic acid unit.
25843830	0	19	theme	cell	85:88	arg1	wall					90:93	Eucalyptus cell wall	74:93	Eucalyptus cell wall	74:93	Structural characterization of hemicelluloses and topochemical changes in Eucalyptus cell wall during alkali ethanol treatment.
25843830	5	20	from	regions	1031:1037	arg1	different					1000:1008	different	1000:1008	different	1000:1008	Raman analysis revealed that the dissolution of hemicelluloses was different in the morphological regions, and the hemicelluloses released mainly originated from the secondary wall.
25843830	5	20	from	regions	1031:1037	arg1	dissolution					966:976	the dissolution	962:976	the dissolution of hemicelluloses	962:994	Raman analysis revealed that the dissolution of hemicelluloses was different in the morphological regions, and the hemicelluloses released mainly originated from the secondary wall.
25843830	2	21	theme	chromatography	452:465	arg1	spectroscopies					527:540	high-performance anion-exchange chromatography, gel permeation chromatography, Fourier transform infrared, and nuclear magnetic resonance spectroscopies	389:540	high-performance anion-exchange chromatography, gel permeation chromatography, Fourier transform infrared, and nuclear magnetic resonance spectroscopies	389:540	The chemical composition and structural features of the hemicellulosic fractions obtained were comparatively characterized by the combination of high-performance anion-exchange chromatography, gel permeation chromatography, Fourier transform infrared, and nuclear magnetic resonance spectroscopies.
25843830	6	22	theme	important	1233:1241	arg1	basis					1243:1247	important basis	1233:1247	important basis for studying the mechanism of the alkali treatment	1233:1298	The information obtained from the study conducted by combining chemical characterization with ultrastructure provides important basis for studying the mechanism of the alkali treatment.
25843830	2	23	theme	structural	273:282	arg1	features					284:291	structural features	273:291	structural features	273:291	The chemical composition and structural features of the hemicellulosic fractions obtained were comparatively characterized by the combination of high-performance anion-exchange chromatography, gel permeation chromatography, Fourier transform infrared, and nuclear magnetic resonance spectroscopies.
25843830	4	24	theme	linear	807:812	arg1	backbone					814:821	a linear backbone	805:821	a linear backbone of (1→4)-linked-β-d-xylopyranosyl residues decorated with branch at O-2 of 4-O-methyl-α-glucuronic acid unit	805:930	Based on the Fourier transform infrared and nuclear magnetic resonance analyses, the hemicelluloses extracted from Eucalyptus mainly have a linear backbone of (1→4)-linked-β-d-xylopyranosyl residues decorated with branch at O-2 of 4-O-methyl-α-glucuronic acid unit.
25843830	4	25	theme	acid	922:925	arg1	unit					927:930	4-O-methyl-α-glucuronic acid unit	898:930	4-O-methyl-α-glucuronic acid unit	898:930	Based on the Fourier transform infrared and nuclear magnetic resonance analyses, the hemicelluloses extracted from Eucalyptus mainly have a linear backbone of (1→4)-linked-β-d-xylopyranosyl residues decorated with branch at O-2 of 4-O-methyl-α-glucuronic acid unit.
25843830	2	26	theme	permeation	441:450	arg1	chromatography					452:465	gel permeation chromatography	437:465	gel permeation chromatography	437:465	The chemical composition and structural features of the hemicellulosic fractions obtained were comparatively characterized by the combination of high-performance anion-exchange chromatography, gel permeation chromatography, Fourier transform infrared, and nuclear magnetic resonance spectroscopies.
25843830	4	27	contain	have	800:803	arg2	backbone					814:821	a linear backbone	805:821	a linear backbone of (1→4)-linked-β-d-xylopyranosyl residues decorated with branch at O-2 of 4-O-methyl-α-glucuronic acid unit	805:930	Based on the Fourier transform infrared and nuclear magnetic resonance analyses, the hemicelluloses extracted from Eucalyptus mainly have a linear backbone of (1→4)-linked-β-d-xylopyranosyl residues decorated with branch at O-2 of 4-O-methyl-α-glucuronic acid unit.
25843830	4	27	contain	have	800:803	arg1	hemicelluloses					752:765	the hemicelluloses	748:765	the hemicelluloses extracted from Eucalyptus	748:791	Based on the Fourier transform infrared and nuclear magnetic resonance analyses, the hemicelluloses extracted from Eucalyptus mainly have a linear backbone of (1→4)-linked-β-d-xylopyranosyl residues decorated with branch at O-2 of 4-O-methyl-α-glucuronic acid unit.
25843830	2	28	theme	gel	437:439	arg1	chromatography					452:465	gel permeation chromatography	437:465	gel permeation chromatography	437:465	The chemical composition and structural features of the hemicellulosic fractions obtained were comparatively characterized by the combination of high-performance anion-exchange chromatography, gel permeation chromatography, Fourier transform infrared, and nuclear magnetic resonance spectroscopies.
25843830	6	29	theme	alkali	1283:1288	arg1	treatment					1290:1298	the alkali treatment	1279:1298	the alkali treatment	1279:1298	The information obtained from the study conducted by combining chemical characterization with ultrastructure provides important basis for studying the mechanism of the alkali treatment.
25843830	5	30	theme	morphological	1017:1029	arg1	regions					1031:1037	the morphological regions	1013:1037	the morphological regions	1013:1037	Raman analysis revealed that the dissolution of hemicelluloses was different in the morphological regions, and the hemicelluloses released mainly originated from the secondary wall.
25843830	5	31	theme	secondary	1099:1107	arg1	wall					1109:1112	the secondary wall	1095:1112	the secondary wall	1095:1112	Raman analysis revealed that the dissolution of hemicelluloses was different in the morphological regions, and the hemicelluloses released mainly originated from the secondary wall.
25843830	2	32	theme	chromatography	421:434	arg1	spectroscopies					527:540	high-performance anion-exchange chromatography, gel permeation chromatography, Fourier transform infrared, and nuclear magnetic resonance spectroscopies	389:540	high-performance anion-exchange chromatography, gel permeation chromatography, Fourier transform infrared, and nuclear magnetic resonance spectroscopies	389:540	The chemical composition and structural features of the hemicellulosic fractions obtained were comparatively characterized by the combination of high-performance anion-exchange chromatography, gel permeation chromatography, Fourier transform infrared, and nuclear magnetic resonance spectroscopies.
25843830	0	33	theme	topochemical	50:61	arg1	changes					63:69	topochemical changes	50:69	topochemical changes in Eucalyptus cell wall during alkali ethanol treatment	50:125	Structural characterization of hemicelluloses and topochemical changes in Eucalyptus cell wall during alkali ethanol treatment.
25843830	2	34	dep	composition	257:267	arg1	The					244:246	The	244:246	The	244:246	The chemical composition and structural features of the hemicellulosic fractions obtained were comparatively characterized by the combination of high-performance anion-exchange chromatography, gel permeation chromatography, Fourier transform infrared, and nuclear magnetic resonance spectroscopies.
25843830	0	35	from	wall	90:93	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization of hemicelluloses and topochemical changes in Eucalyptus cell wall during alkali ethanol treatment.	0:126	Structural characterization of hemicelluloses and topochemical changes in Eucalyptus cell wall during alkali ethanol treatment.
25843830	5	36	theme	Raman	933:937	arg1	analysis					939:946	Raman analysis	933:946	Raman analysis	933:946	Raman analysis revealed that the dissolution of hemicelluloses was different in the morphological regions, and the hemicelluloses released mainly originated from the secondary wall.
25843830	2	37	theme	anion-exchange	406:419	arg1	chromatography					421:434	high-performance anion-exchange chromatography	389:434	high-performance anion-exchange chromatography	389:434	The chemical composition and structural features of the hemicellulosic fractions obtained were comparatively characterized by the combination of high-performance anion-exchange chromatography, gel permeation chromatography, Fourier transform infrared, and nuclear magnetic resonance spectroscopies.
25843830	0	38	theme	hemicelluloses	31:44	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization of hemicelluloses and topochemical changes in Eucalyptus cell wall during alkali ethanol treatment.	0:126	Structural characterization of hemicelluloses and topochemical changes in Eucalyptus cell wall during alkali ethanol treatment.
25843830	2	39	theme	spectroscopies	527:540	arg1	combination					374:384	the combination	370:384	the combination of high-performance anion-exchange chromatography, gel permeation chromatography, Fourier transform infrared, and nuclear magnetic resonance spectroscopies	370:540	The chemical composition and structural features of the hemicellulosic fractions obtained were comparatively characterized by the combination of high-performance anion-exchange chromatography, gel permeation chromatography, Fourier transform infrared, and nuclear magnetic resonance spectroscopies.
25843830	1	40	contain	containing	183:192	arg2	NaOH					223:226	0.4, 1.0, 2.0, 3.0, and 5.0% NaOH	194:226	0.4, 1.0, 2.0, 3.0, and 5.0% NaOH for 2h	194:233	Eucalyptus was sequentially extracted with 70% ethanol containing 0.4, 1.0, 2.0, 3.0, and 5.0% NaOH for 2h at 80°C.
25843830	1	40	contain	containing	183:192	arg1	ethanol					175:181	70% ethanol	171:181	70% ethanol containing 0.4, 1.0, 2.0, 3.0, and 5.0% NaOH for 2h at 80°C	171:241	Eucalyptus was sequentially extracted with 70% ethanol containing 0.4, 1.0, 2.0, 3.0, and 5.0% NaOH for 2h at 80°C.
25843830	3	41	theme	cell	609:612	arg1	wall					614:617	cell wall	609:617	cell wall	609:617	Furthermore, the main component distribution and their changes in cell wall were investigated by confocal Raman microscopy.
25843830	2	42	theme	fractions	315:323	arg1	composition					257:267	chemical composition	248:267	chemical composition	248:267	The chemical composition and structural features of the hemicellulosic fractions obtained were comparatively characterized by the combination of high-performance anion-exchange chromatography, gel permeation chromatography, Fourier transform infrared, and nuclear magnetic resonance spectroscopies.
25843830	2	42	theme	fractions	315:323	arg1	features					284:291	structural features	273:291	structural features	273:291	The chemical composition and structural features of the hemicellulosic fractions obtained were comparatively characterized by the combination of high-performance anion-exchange chromatography, gel permeation chromatography, Fourier transform infrared, and nuclear magnetic resonance spectroscopies.
25843830	2	43	theme	Fourier	468:474	arg1	spectroscopies					527:540	high-performance anion-exchange chromatography, gel permeation chromatography, Fourier transform infrared, and nuclear magnetic resonance spectroscopies	389:540	high-performance anion-exchange chromatography, gel permeation chromatography, Fourier transform infrared, and nuclear magnetic resonance spectroscopies	389:540	The chemical composition and structural features of the hemicellulosic fractions obtained were comparatively characterized by the combination of high-performance anion-exchange chromatography, gel permeation chromatography, Fourier transform infrared, and nuclear magnetic resonance spectroscopies.
25843830	2	44	theme	hemicellulosic	300:313	arg1	fractions					315:323	the hemicellulosic fractions	296:323	the hemicellulosic fractions obtained	296:332	The chemical composition and structural features of the hemicellulosic fractions obtained were comparatively characterized by the combination of high-performance anion-exchange chromatography, gel permeation chromatography, Fourier transform infrared, and nuclear magnetic resonance spectroscopies.
25843830	4	45	dep	Fourier	680:686	arg1	transform					688:696	transform	688:696	transform infrared	688:705	Based on the Fourier transform infrared and nuclear magnetic resonance analyses, the hemicelluloses extracted from Eucalyptus mainly have a linear backbone of (1→4)-linked-β-d-xylopyranosyl residues decorated with branch at O-2 of 4-O-methyl-α-glucuronic acid unit.
25843830	4	45	dep	Fourier	680:686	arg1	analyses					738:745	analyses	738:745	analyses	738:745	Based on the Fourier transform infrared and nuclear magnetic resonance analyses, the hemicelluloses extracted from Eucalyptus mainly have a linear backbone of (1→4)-linked-β-d-xylopyranosyl residues decorated with branch at O-2 of 4-O-methyl-α-glucuronic acid unit.
25843830	4	46	theme	1→4	827:829	arg1	residues					857:864	(1→4)-linked-β-d-xylopyranosyl residues	826:864	(1→4)-linked-β-d-xylopyranosyl residues decorated with branch at O-2 of 4-O-methyl-α-glucuronic acid unit	826:930	Based on the Fourier transform infrared and nuclear magnetic resonance analyses, the hemicelluloses extracted from Eucalyptus mainly have a linear backbone of (1→4)-linked-β-d-xylopyranosyl residues decorated with branch at O-2 of 4-O-methyl-α-glucuronic acid unit.
25843830	3	47	theme	Raman	649:653	arg1	microscopy					655:664	confocal Raman microscopy	640:664	confocal Raman microscopy	640:664	Furthermore, the main component distribution and their changes in cell wall were investigated by confocal Raman microscopy.
25843830	4	48	theme	residues	857:864	arg1	backbone					814:821	a linear backbone	805:821	a linear backbone of (1→4)-linked-β-d-xylopyranosyl residues decorated with branch at O-2 of 4-O-methyl-α-glucuronic acid unit	805:930	Based on the Fourier transform infrared and nuclear magnetic resonance analyses, the hemicelluloses extracted from Eucalyptus mainly have a linear backbone of (1→4)-linked-β-d-xylopyranosyl residues decorated with branch at O-2 of 4-O-methyl-α-glucuronic acid unit.
25843830	5	49	theme	hemicelluloses	981:994	arg1	different					1000:1008	different	1000:1008	different	1000:1008	Raman analysis revealed that the dissolution of hemicelluloses was different in the morphological regions, and the hemicelluloses released mainly originated from the secondary wall.
25843830	5	49	theme	hemicelluloses	981:994	arg1	dissolution					966:976	the dissolution	962:976	the dissolution of hemicelluloses	962:994	Raman analysis revealed that the dissolution of hemicelluloses was different in the morphological regions, and the hemicelluloses released mainly originated from the secondary wall.
25843830	5	50	dep	released	1063:1070	arg1	originated					1079:1088	originated	1079:1088	released mainly originated from the secondary wall	1063:1112	Raman analysis revealed that the dissolution of hemicelluloses was different in the morphological regions, and the hemicelluloses released mainly originated from the secondary wall.
25843830	3	51	theme	main	560:563	arg1	distribution					575:586	the main component distribution	556:586	the main component distribution	556:586	Furthermore, the main component distribution and their changes in cell wall were investigated by confocal Raman microscopy.
25843830	3	52	theme	component	565:573	arg1	distribution					575:586	the main component distribution	556:586	the main component distribution	556:586	Furthermore, the main component distribution and their changes in cell wall were investigated by confocal Raman microscopy.
25843830	2	53	theme	magnetic	508:515	arg1	resonance					517:525	nuclear magnetic resonance	500:525	nuclear magnetic resonance	500:525	The chemical composition and structural features of the hemicellulosic fractions obtained were comparatively characterized by the combination of high-performance anion-exchange chromatography, gel permeation chromatography, Fourier transform infrared, and nuclear magnetic resonance spectroscopies.
25843830	3	54	from	changes	598:604	arg1	wall					614:617	cell wall	609:617	cell wall	609:617	Furthermore, the main component distribution and their changes in cell wall were investigated by confocal Raman microscopy.
25843830	6	55	theme	treatment	1290:1298	arg1	mechanism					1266:1274	the mechanism	1262:1274	the mechanism of the alkali treatment	1262:1298	The information obtained from the study conducted by combining chemical characterization with ultrastructure provides important basis for studying the mechanism of the alkali treatment.
25843830	2	56	theme	resonance	517:525	arg1	spectroscopies					527:540	high-performance anion-exchange chromatography, gel permeation chromatography, Fourier transform infrared, and nuclear magnetic resonance spectroscopies	389:540	high-performance anion-exchange chromatography, gel permeation chromatography, Fourier transform infrared, and nuclear magnetic resonance spectroscopies	389:540	The chemical composition and structural features of the hemicellulosic fractions obtained were comparatively characterized by the combination of high-performance anion-exchange chromatography, gel permeation chromatography, Fourier transform infrared, and nuclear magnetic resonance spectroscopies.
25843830	2	57	theme	high-performance	389:404	arg1	chromatography					421:434	high-performance anion-exchange chromatography	389:434	high-performance anion-exchange chromatography	389:434	The chemical composition and structural features of the hemicellulosic fractions obtained were comparatively characterized by the combination of high-performance anion-exchange chromatography, gel permeation chromatography, Fourier transform infrared, and nuclear magnetic resonance spectroscopies.
25843830	4	58	theme	-linked-β-d-xylopyranosyl	831:855	arg1	residues					857:864	(1→4)-linked-β-d-xylopyranosyl residues	826:864	(1→4)-linked-β-d-xylopyranosyl residues decorated with branch at O-2 of 4-O-methyl-α-glucuronic acid unit	826:930	Based on the Fourier transform infrared and nuclear magnetic resonance analyses, the hemicelluloses extracted from Eucalyptus mainly have a linear backbone of (1→4)-linked-β-d-xylopyranosyl residues decorated with branch at O-2 of 4-O-methyl-α-glucuronic acid unit.
25843830	4	59	theme	magnetic	719:726	arg1	resonance					728:736	nuclear magnetic resonance	711:736	nuclear magnetic resonance	711:736	Based on the Fourier transform infrared and nuclear magnetic resonance analyses, the hemicelluloses extracted from Eucalyptus mainly have a linear backbone of (1→4)-linked-β-d-xylopyranosyl residues decorated with branch at O-2 of 4-O-methyl-α-glucuronic acid unit.
25843830	0	60	theme	Eucalyptus	74:83	arg1	wall					90:93	Eucalyptus cell wall	74:93	Eucalyptus cell wall	74:93	Structural characterization of hemicelluloses and topochemical changes in Eucalyptus cell wall during alkali ethanol treatment.
25843830	0	61	from	hemicelluloses	31:44	arg1	wall					90:93	Eucalyptus cell wall	74:93	Eucalyptus cell wall	74:93	Structural characterization of hemicelluloses and topochemical changes in Eucalyptus cell wall during alkali ethanol treatment.
25843830	3	62	theme	confocal	640:647	arg1	microscopy					655:664	confocal Raman microscopy	640:664	confocal Raman microscopy	640:664	Furthermore, the main component distribution and their changes in cell wall were investigated by confocal Raman microscopy.
25843830	1	63	theme	70	171:172	arg1	%					173:173	%	173:173	%	173:173	Eucalyptus was sequentially extracted with 70% ethanol containing 0.4, 1.0, 2.0, 3.0, and 5.0% NaOH for 2h at 80°C.
25843830	1	64	theme	%	221:221	arg1	NaOH					223:226	0.4, 1.0, 2.0, 3.0, and 5.0% NaOH	194:226	0.4, 1.0, 2.0, 3.0, and 5.0% NaOH for 2h	194:233	Eucalyptus was sequentially extracted with 70% ethanol containing 0.4, 1.0, 2.0, 3.0, and 5.0% NaOH for 2h at 80°C.
29021489	5	0	theme	Confocal	735:742	arg1	CLSM					771:774	CLSM	771:774	CLSM	771:774	Confocal laser scanning microscopy (CLSM) images of the cosmetic film showed that MPM-GDM spread through the whole film in the absence of glycerin, whereas the addition of glycerin led to the formation of a sea-island structure.
29021489	5	0	theme	Confocal	735:742	arg1	microscopy					759:768	Confocal laser scanning microscopy	735:768	Confocal laser scanning microscopy (CLSM) images of the cosmetic film	735:803	Confocal laser scanning microscopy (CLSM) images of the cosmetic film showed that MPM-GDM spread through the whole film in the absence of glycerin, whereas the addition of glycerin led to the formation of a sea-island structure.
29021489	7	1	theme	gum	1258:1260	arg1	concentration					1216:1228	the concentration	1212:1228	the concentration of both MPM-GDM and xanthan gum	1212:1260	We also demonstrated that the concentration of both MPM-GDM and xanthan gum affected the surface morphology.
29021489	1	2	theme	diisostearate	267:279	arg1	MPM-GDM					305:311	MPM-GDM	305:311	MPM-GDM	305:311	The surface morphology of a cosmetic film consisting of an amphiphilic random copolymer (methoxy polyethylene glycol-23 methacrylate/glyceryl diisostearate methacrylate copolymer, MPM-GDM) and solvents has been studied.
29021489	1	2	theme	diisostearate	267:279	arg1	copolymer					294:302	methoxy polyethylene glycol-23 methacrylate/glyceryl diisostearate methacrylate copolymer	214:302	methoxy polyethylene glycol-23 methacrylate/glyceryl diisostearate methacrylate copolymer	214:302	The surface morphology of a cosmetic film consisting of an amphiphilic random copolymer (methoxy polyethylene glycol-23 methacrylate/glyceryl diisostearate methacrylate copolymer, MPM-GDM) and solvents has been studied.
29021489	5	3	theme	laser	744:748	arg1	CLSM					771:774	CLSM	771:774	CLSM	771:774	Confocal laser scanning microscopy (CLSM) images of the cosmetic film showed that MPM-GDM spread through the whole film in the absence of glycerin, whereas the addition of glycerin led to the formation of a sea-island structure.
29021489	5	3	theme	laser	744:748	arg1	microscopy					759:768	Confocal laser scanning microscopy	735:768	Confocal laser scanning microscopy (CLSM) images of the cosmetic film	735:803	Confocal laser scanning microscopy (CLSM) images of the cosmetic film showed that MPM-GDM spread through the whole film in the absence of glycerin, whereas the addition of glycerin led to the formation of a sea-island structure.
29021489	3	4	from	water	506:510	arg1	soluble					495:501	soluble	495:501	soluble	495:501	MPM-GDM was soluble in water, monohydric alcohols, and 1,3-butylene glycol (1,3-BG), whereas it hardly dissolved in glycerin.
29021489	1	5	theme	cosmetic	153:160	arg1	film					162:165	a cosmetic film	151:165	a cosmetic film consisting of an amphiphilic random copolymer (methoxy polyethylene glycol-23 methacrylate/glyceryl diisostearate methacrylate copolymer, MPM-GDM) and solvents	151:325	The surface morphology of a cosmetic film consisting of an amphiphilic random copolymer (methoxy polyethylene glycol-23 methacrylate/glyceryl diisostearate methacrylate copolymer, MPM-GDM) and solvents has been studied.
29021489	1	6	theme	methacrylate	281:292	arg1	MPM-GDM					305:311	MPM-GDM	305:311	MPM-GDM	305:311	The surface morphology of a cosmetic film consisting of an amphiphilic random copolymer (methoxy polyethylene glycol-23 methacrylate/glyceryl diisostearate methacrylate copolymer, MPM-GDM) and solvents has been studied.
29021489	1	6	theme	methacrylate	281:292	arg1	copolymer					294:302	methoxy polyethylene glycol-23 methacrylate/glyceryl diisostearate methacrylate copolymer	214:302	methoxy polyethylene glycol-23 methacrylate/glyceryl diisostearate methacrylate copolymer	214:302	The surface morphology of a cosmetic film consisting of an amphiphilic random copolymer (methoxy polyethylene glycol-23 methacrylate/glyceryl diisostearate methacrylate copolymer, MPM-GDM) and solvents has been studied.
29021489	6	7	theme	MPM-GDM	1114:1120	arg1	miscibility					1070:1080	the miscibility	1066:1080	the miscibility (or the interfacial tension) of MPM-GDM against the solvents	1066:1141	It was assumed that the size of the MPM-GDM domain was determined by the balance between two factors: the miscibility (or the interfacial tension) of MPM-GDM against the solvents and the viscosity of the continuous phase.
29021489	6	7	theme	MPM-GDM	1114:1120	arg1	viscosity					1151:1159	the viscosity	1147:1159	the viscosity of the continuous phase	1147:1183	It was assumed that the size of the MPM-GDM domain was determined by the balance between two factors: the miscibility (or the interfacial tension) of MPM-GDM against the solvents and the viscosity of the continuous phase.
29021489	6	7	theme	MPM-GDM	1114:1120	arg1	factors					1057:1063	two factors	1053:1063	two factors: the miscibility (or the interfacial tension) of MPM-GDM against the solvents and the viscosity of the continuous phase	1053:1183	It was assumed that the size of the MPM-GDM domain was determined by the balance between two factors: the miscibility (or the interfacial tension) of MPM-GDM against the solvents and the viscosity of the continuous phase.
29021489	6	7	theme	MPM-GDM	1114:1120	arg1	tension					1102:1108	the interfacial tension	1086:1108	the interfacial tension	1086:1108	It was assumed that the size of the MPM-GDM domain was determined by the balance between two factors: the miscibility (or the interfacial tension) of MPM-GDM against the solvents and the viscosity of the continuous phase.
29021489	8	8	theme	morphology	1318:1327	arg1	Control					1295:1301	Control	1295:1301	Control of the surface morphology by changing the solubility of MPM-GDM	1295:1365	Control of the surface morphology by changing the solubility of MPM-GDM is expected to be useful for improving the functionality and feel of cosmetic films.
29021489	3	9	theme	1,3-butylene	538:549	arg1	1,3-BG					559:564	1,3-BG	559:564	1,3-BG	559:564	MPM-GDM was soluble in water, monohydric alcohols, and 1,3-butylene glycol (1,3-BG), whereas it hardly dissolved in glycerin.
29021489	3	9	theme	1,3-butylene	538:549	arg1	glycol					551:556	1,3-butylene glycol	538:556	1,3-butylene glycol (1,3-BG)	538:565	MPM-GDM was soluble in water, monohydric alcohols, and 1,3-butylene glycol (1,3-BG), whereas it hardly dissolved in glycerin.
29021489	3	10	from	glycol	551:556	arg1	soluble					495:501	soluble	495:501	soluble	495:501	MPM-GDM was soluble in water, monohydric alcohols, and 1,3-butylene glycol (1,3-BG), whereas it hardly dissolved in glycerin.
29021489	0	11	theme	Xanthan	98:104	arg1	Gum					106:108	Xanthan Gum	98:108	Xanthan Gum	98:108	Surface Morphology of Cosmetic Film Consisting of PEG-Diisostearate Amphiphilic Random Copolymer, Xanthan Gum, and Solvents.
29021489	4	12	theme	surface	613:619	arg1	morphology					621:630	The surface morphology	609:630	The surface morphology	609:630	The surface morphology was examined by changing the solvent composition of 1,3-BG (good solvent) and glycerin (poor solvent).
29021489	6	13	theme	phase	1179:1183	arg1	miscibility					1070:1080	the miscibility	1066:1080	the miscibility (or the interfacial tension) of MPM-GDM against the solvents	1066:1141	It was assumed that the size of the MPM-GDM domain was determined by the balance between two factors: the miscibility (or the interfacial tension) of MPM-GDM against the solvents and the viscosity of the continuous phase.
29021489	6	13	theme	phase	1179:1183	arg1	viscosity					1151:1159	the viscosity	1147:1159	the viscosity of the continuous phase	1147:1183	It was assumed that the size of the MPM-GDM domain was determined by the balance between two factors: the miscibility (or the interfacial tension) of MPM-GDM against the solvents and the viscosity of the continuous phase.
29021489	6	13	theme	phase	1179:1183	arg1	factors					1057:1063	two factors	1053:1063	two factors: the miscibility (or the interfacial tension) of MPM-GDM against the solvents and the viscosity of the continuous phase	1053:1183	It was assumed that the size of the MPM-GDM domain was determined by the balance between two factors: the miscibility (or the interfacial tension) of MPM-GDM against the solvents and the viscosity of the continuous phase.
29021489	6	13	theme	phase	1179:1183	arg1	tension					1102:1108	the interfacial tension	1086:1108	the interfacial tension	1086:1108	It was assumed that the size of the MPM-GDM domain was determined by the balance between two factors: the miscibility (or the interfacial tension) of MPM-GDM against the solvents and the viscosity of the continuous phase.
29021489	4	14	dep	1,3-BG	684:689	arg1	solvent					697:703	solvent	697:703	solvent	697:703	The surface morphology was examined by changing the solvent composition of 1,3-BG (good solvent) and glycerin (poor solvent).
29021489	4	15	dep	glycerin	710:717	arg1	solvent					725:731	solvent	725:731	solvent	725:731	The surface morphology was examined by changing the solvent composition of 1,3-BG (good solvent) and glycerin (poor solvent).
29021489	5	16	theme	film	800:803	arg1	images					777:782	Confocal laser scanning microscopy (CLSM) images	735:782	Confocal laser scanning microscopy (CLSM) images of the cosmetic film	735:803	Confocal laser scanning microscopy (CLSM) images of the cosmetic film showed that MPM-GDM spread through the whole film in the absence of glycerin, whereas the addition of glycerin led to the formation of a sea-island structure.
29021489	3	17	from	alcohols	524:531	arg1	soluble					495:501	soluble	495:501	soluble	495:501	MPM-GDM was soluble in water, monohydric alcohols, and 1,3-butylene glycol (1,3-BG), whereas it hardly dissolved in glycerin.
29021489	4	18	theme	solvent	661:667	arg1	composition					669:679	the solvent composition	657:679	the solvent composition of 1,3-BG (good solvent) and glycerin (poor solvent)	657:732	The surface morphology was examined by changing the solvent composition of 1,3-BG (good solvent) and glycerin (poor solvent).
29021489	1	19	theme	film	162:165	arg1	morphology					137:146	The surface morphology	125:146	The surface morphology of a cosmetic film consisting of an amphiphilic random copolymer (methoxy polyethylene glycol-23 methacrylate/glyceryl diisostearate methacrylate copolymer, MPM-GDM) and solvents	125:325	The surface morphology of a cosmetic film consisting of an amphiphilic random copolymer (methoxy polyethylene glycol-23 methacrylate/glyceryl diisostearate methacrylate copolymer, MPM-GDM) and solvents has been studied.
29021489	0	20	theme	Surface	0:6	arg1	Morphology					8:17	Surface Morphology	0:17	Surface Morphology of Cosmetic Film	0:34	Surface Morphology of Cosmetic Film Consisting of PEG-Diisostearate Amphiphilic Random Copolymer, Xanthan Gum, and Solvents.
29021489	6	21	theme	MPM-GDM	1000:1006	arg1	domain					1008:1013	the MPM-GDM domain	996:1013	the MPM-GDM domain	996:1013	It was assumed that the size of the MPM-GDM domain was determined by the balance between two factors: the miscibility (or the interfacial tension) of MPM-GDM against the solvents and the viscosity of the continuous phase.
29021489	2	22	theme	gum	464:466	arg1	mixture					436:442	a homogeneous aqueous mixture	414:442	a homogeneous aqueous mixture of MPM-GDM, xanthan gum, and solvents	414:480	The cosmetic film was prepared through the evaporation of water from a homogeneous aqueous mixture of MPM-GDM, xanthan gum, and solvents.
29021489	8	23	theme	cosmetic	1436:1443	arg1	films					1445:1449	cosmetic films	1436:1449	cosmetic films	1436:1449	Control of the surface morphology by changing the solubility of MPM-GDM is expected to be useful for improving the functionality and feel of cosmetic films.
29021489	6	24	theme	domain	1008:1013	arg1	size					988:991	the size	984:991	the size of the MPM-GDM domain	984:1013	It was assumed that the size of the MPM-GDM domain was determined by the balance between two factors: the miscibility (or the interfacial tension) of MPM-GDM against the solvents and the viscosity of the continuous phase.
29021489	2	25	theme	water	403:407	arg1	evaporation					388:398	the evaporation	384:398	the evaporation of water	384:407	The cosmetic film was prepared through the evaporation of water from a homogeneous aqueous mixture of MPM-GDM, xanthan gum, and solvents.
29021489	2	26	theme	xanthan	456:462	arg1	gum					464:466	xanthan gum	456:466	xanthan gum	456:466	The cosmetic film was prepared through the evaporation of water from a homogeneous aqueous mixture of MPM-GDM, xanthan gum, and solvents.
29021489	6	27	theme	continuous	1168:1177	arg1	phase					1179:1183	the continuous phase	1164:1183	the continuous phase	1164:1183	It was assumed that the size of the MPM-GDM domain was determined by the balance between two factors: the miscibility (or the interfacial tension) of MPM-GDM against the solvents and the viscosity of the continuous phase.
29021489	3	28	from	soluble	495:501	arg1	1,3-BG					559:564	1,3-BG	559:564	1,3-BG	559:564	MPM-GDM was soluble in water, monohydric alcohols, and 1,3-butylene glycol (1,3-BG), whereas it hardly dissolved in glycerin.
29021489	3	28	from	soluble	495:501	arg1	glycol					551:556	1,3-butylene glycol	538:556	1,3-butylene glycol (1,3-BG)	538:565	MPM-GDM was soluble in water, monohydric alcohols, and 1,3-butylene glycol (1,3-BG), whereas it hardly dissolved in glycerin.
29021489	3	28	from	soluble	495:501	arg1	water					506:510	water	506:510	water	506:510	MPM-GDM was soluble in water, monohydric alcohols, and 1,3-butylene glycol (1,3-BG), whereas it hardly dissolved in glycerin.
29021489	3	28	from	soluble	495:501	arg1	alcohols					524:531	monohydric alcohols	513:531	monohydric alcohols	513:531	MPM-GDM was soluble in water, monohydric alcohols, and 1,3-butylene glycol (1,3-BG), whereas it hardly dissolved in glycerin.
29021489	4	29	theme	glycerin	710:717	arg1	composition					669:679	the solvent composition	657:679	the solvent composition of 1,3-BG (good solvent) and glycerin (poor solvent)	657:732	The surface morphology was examined by changing the solvent composition of 1,3-BG (good solvent) and glycerin (poor solvent).
29021489	0	30	theme	Cosmetic	22:29	arg1	Film					31:34	Cosmetic Film	22:34	Cosmetic Film	22:34	Surface Morphology of Cosmetic Film Consisting of PEG-Diisostearate Amphiphilic Random Copolymer, Xanthan Gum, and Solvents.
29021489	7	31	theme	xanthan	1250:1256	arg1	gum					1258:1260	xanthan gum	1250:1260	xanthan gum	1250:1260	We also demonstrated that the concentration of both MPM-GDM and xanthan gum affected the surface morphology.
29021489	5	32	from	film	850:853	arg1	absence					862:868	the absence	858:868	the absence of glycerin	858:880	Confocal laser scanning microscopy (CLSM) images of the cosmetic film showed that MPM-GDM spread through the whole film in the absence of glycerin, whereas the addition of glycerin led to the formation of a sea-island structure.
29021489	1	33	theme	amphiphilic	184:194	arg1	copolymer					203:211	an amphiphilic random copolymer	181:211	an amphiphilic random copolymer (methoxy polyethylene glycol-23 methacrylate/glyceryl diisostearate methacrylate copolymer, MPM-GDM)	181:312	The surface morphology of a cosmetic film consisting of an amphiphilic random copolymer (methoxy polyethylene glycol-23 methacrylate/glyceryl diisostearate methacrylate copolymer, MPM-GDM) and solvents has been studied.
29021489	2	34	theme	MPM-GDM	447:453	arg1	mixture					436:442	a homogeneous aqueous mixture	414:442	a homogeneous aqueous mixture of MPM-GDM, xanthan gum, and solvents	414:480	The cosmetic film was prepared through the evaporation of water from a homogeneous aqueous mixture of MPM-GDM, xanthan gum, and solvents.
29021489	8	35	theme	MPM-GDM	1359:1365	arg1	solubility					1345:1354	the solubility	1341:1354	the solubility of MPM-GDM	1341:1365	Control of the surface morphology by changing the solubility of MPM-GDM is expected to be useful for improving the functionality and feel of cosmetic films.
29021489	4	36	theme	1,3-BG	684:689	arg1	composition					669:679	the solvent composition	657:679	the solvent composition of 1,3-BG (good solvent) and glycerin (poor solvent)	657:732	The surface morphology was examined by changing the solvent composition of 1,3-BG (good solvent) and glycerin (poor solvent).
29021489	1	37	theme	random	196:201	arg1	copolymer					203:211	an amphiphilic random copolymer	181:211	an amphiphilic random copolymer (methoxy polyethylene glycol-23 methacrylate/glyceryl diisostearate methacrylate copolymer, MPM-GDM)	181:312	The surface morphology of a cosmetic film consisting of an amphiphilic random copolymer (methoxy polyethylene glycol-23 methacrylate/glyceryl diisostearate methacrylate copolymer, MPM-GDM) and solvents has been studied.
29021489	5	38	theme	scanning	750:757	arg1	CLSM					771:774	CLSM	771:774	CLSM	771:774	Confocal laser scanning microscopy (CLSM) images of the cosmetic film showed that MPM-GDM spread through the whole film in the absence of glycerin, whereas the addition of glycerin led to the formation of a sea-island structure.
29021489	5	38	theme	scanning	750:757	arg1	microscopy					759:768	Confocal laser scanning microscopy	735:768	Confocal laser scanning microscopy (CLSM) images of the cosmetic film	735:803	Confocal laser scanning microscopy (CLSM) images of the cosmetic film showed that MPM-GDM spread through the whole film in the absence of glycerin, whereas the addition of glycerin led to the formation of a sea-island structure.
29021489	5	39	theme	structure	953:961	arg1	formation					927:935	the formation	923:935	the formation of a sea-island structure	923:961	Confocal laser scanning microscopy (CLSM) images of the cosmetic film showed that MPM-GDM spread through the whole film in the absence of glycerin, whereas the addition of glycerin led to the formation of a sea-island structure.
29021489	2	40	theme	aqueous	428:434	arg1	mixture					436:442	a homogeneous aqueous mixture	414:442	a homogeneous aqueous mixture of MPM-GDM, xanthan gum, and solvents	414:480	The cosmetic film was prepared through the evaporation of water from a homogeneous aqueous mixture of MPM-GDM, xanthan gum, and solvents.
29021489	0	41	theme	Film	31:34	arg1	Morphology					8:17	Surface Morphology	0:17	Surface Morphology of Cosmetic Film	0:34	Surface Morphology of Cosmetic Film Consisting of PEG-Diisostearate Amphiphilic Random Copolymer, Xanthan Gum, and Solvents.
29021489	2	42	theme	solvents	473:480	arg1	mixture					436:442	a homogeneous aqueous mixture	414:442	a homogeneous aqueous mixture of MPM-GDM, xanthan gum, and solvents	414:480	The cosmetic film was prepared through the evaporation of water from a homogeneous aqueous mixture of MPM-GDM, xanthan gum, and solvents.
29021489	7	43	theme	MPM-GDM	1238:1244	arg1	concentration					1216:1228	the concentration	1212:1228	the concentration of both MPM-GDM and xanthan gum	1212:1260	We also demonstrated that the concentration of both MPM-GDM and xanthan gum affected the surface morphology.
29021489	2	44	theme	homogeneous	416:426	arg1	mixture					436:442	a homogeneous aqueous mixture	414:442	a homogeneous aqueous mixture of MPM-GDM, xanthan gum, and solvents	414:480	The cosmetic film was prepared through the evaporation of water from a homogeneous aqueous mixture of MPM-GDM, xanthan gum, and solvents.
29021489	0	45	theme	PEG-Diisostearate	50:66	arg1	Copolymer					87:95	PEG-Diisostearate Amphiphilic Random Copolymer	50:95	PEG-Diisostearate Amphiphilic Random Copolymer	50:95	Surface Morphology of Cosmetic Film Consisting of PEG-Diisostearate Amphiphilic Random Copolymer, Xanthan Gum, and Solvents.
29021489	5	46	theme	whole	844:848	arg1	film					850:853	the whole film	840:853	the whole film in the absence of glycerin	840:880	Confocal laser scanning microscopy (CLSM) images of the cosmetic film showed that MPM-GDM spread through the whole film in the absence of glycerin, whereas the addition of glycerin led to the formation of a sea-island structure.
29021489	7	47	theme	surface	1275:1281	arg1	morphology					1283:1292	the surface morphology	1271:1292	the surface morphology	1271:1292	We also demonstrated that the concentration of both MPM-GDM and xanthan gum affected the surface morphology.
29021489	6	48	theme	interfacial	1090:1100	arg1	miscibility					1070:1080	the miscibility	1066:1080	the miscibility (or the interfacial tension) of MPM-GDM against the solvents	1066:1141	It was assumed that the size of the MPM-GDM domain was determined by the balance between two factors: the miscibility (or the interfacial tension) of MPM-GDM against the solvents and the viscosity of the continuous phase.
29021489	6	48	theme	interfacial	1090:1100	arg1	tension					1102:1108	the interfacial tension	1086:1108	the interfacial tension	1086:1108	It was assumed that the size of the MPM-GDM domain was determined by the balance between two factors: the miscibility (or the interfacial tension) of MPM-GDM against the solvents and the viscosity of the continuous phase.
29021489	8	49	theme	surface	1310:1316	arg1	morphology					1318:1327	the surface morphology	1306:1327	the surface morphology	1306:1327	Control of the surface morphology by changing the solubility of MPM-GDM is expected to be useful for improving the functionality and feel of cosmetic films.
29021489	5	50	theme	glycerin	873:880	arg1	absence					862:868	the absence	858:868	the absence of glycerin	858:880	Confocal laser scanning microscopy (CLSM) images of the cosmetic film showed that MPM-GDM spread through the whole film in the absence of glycerin, whereas the addition of glycerin led to the formation of a sea-island structure.
29021489	5	51	theme	sea-island	942:951	arg1	structure					953:961	a sea-island structure	940:961	a sea-island structure	940:961	Confocal laser scanning microscopy (CLSM) images of the cosmetic film showed that MPM-GDM spread through the whole film in the absence of glycerin, whereas the addition of glycerin led to the formation of a sea-island structure.
29021489	1	52	dep	copolymer	203:211	arg1	MPM-GDM					305:311	MPM-GDM	305:311	MPM-GDM	305:311	The surface morphology of a cosmetic film consisting of an amphiphilic random copolymer (methoxy polyethylene glycol-23 methacrylate/glyceryl diisostearate methacrylate copolymer, MPM-GDM) and solvents has been studied.
29021489	1	52	dep	copolymer	203:211	arg1	copolymer					294:302	methoxy polyethylene glycol-23 methacrylate/glyceryl diisostearate methacrylate copolymer	214:302	methoxy polyethylene glycol-23 methacrylate/glyceryl diisostearate methacrylate copolymer	214:302	The surface morphology of a cosmetic film consisting of an amphiphilic random copolymer (methoxy polyethylene glycol-23 methacrylate/glyceryl diisostearate methacrylate copolymer, MPM-GDM) and solvents has been studied.
29021489	2	53	theme	cosmetic	349:356	arg1	film					358:361	The cosmetic film	345:361	The cosmetic film	345:361	The cosmetic film was prepared through the evaporation of water from a homogeneous aqueous mixture of MPM-GDM, xanthan gum, and solvents.
29021489	1	54	theme	methoxy	214:220	arg1	MPM-GDM					305:311	MPM-GDM	305:311	MPM-GDM	305:311	The surface morphology of a cosmetic film consisting of an amphiphilic random copolymer (methoxy polyethylene glycol-23 methacrylate/glyceryl diisostearate methacrylate copolymer, MPM-GDM) and solvents has been studied.
29021489	1	54	theme	methoxy	214:220	arg1	copolymer					294:302	methoxy polyethylene glycol-23 methacrylate/glyceryl diisostearate methacrylate copolymer	214:302	methoxy polyethylene glycol-23 methacrylate/glyceryl diisostearate methacrylate copolymer	214:302	The surface morphology of a cosmetic film consisting of an amphiphilic random copolymer (methoxy polyethylene glycol-23 methacrylate/glyceryl diisostearate methacrylate copolymer, MPM-GDM) and solvents has been studied.
29021489	5	55	theme	glycerin	907:914	arg1	addition					895:902	the addition	891:902	the addition of glycerin	891:914	Confocal laser scanning microscopy (CLSM) images of the cosmetic film showed that MPM-GDM spread through the whole film in the absence of glycerin, whereas the addition of glycerin led to the formation of a sea-island structure.
29021489	1	56	theme	surface	129:135	arg1	morphology					137:146	The surface morphology	125:146	The surface morphology of a cosmetic film consisting of an amphiphilic random copolymer (methoxy polyethylene glycol-23 methacrylate/glyceryl diisostearate methacrylate copolymer, MPM-GDM) and solvents	125:325	The surface morphology of a cosmetic film consisting of an amphiphilic random copolymer (methoxy polyethylene glycol-23 methacrylate/glyceryl diisostearate methacrylate copolymer, MPM-GDM) and solvents has been studied.
29021489	1	57	theme	polyethylene	222:233	arg1	MPM-GDM					305:311	MPM-GDM	305:311	MPM-GDM	305:311	The surface morphology of a cosmetic film consisting of an amphiphilic random copolymer (methoxy polyethylene glycol-23 methacrylate/glyceryl diisostearate methacrylate copolymer, MPM-GDM) and solvents has been studied.
29021489	1	57	theme	polyethylene	222:233	arg1	copolymer					294:302	methoxy polyethylene glycol-23 methacrylate/glyceryl diisostearate methacrylate copolymer	214:302	methoxy polyethylene glycol-23 methacrylate/glyceryl diisostearate methacrylate copolymer	214:302	The surface morphology of a cosmetic film consisting of an amphiphilic random copolymer (methoxy polyethylene glycol-23 methacrylate/glyceryl diisostearate methacrylate copolymer, MPM-GDM) and solvents has been studied.
29021489	0	58	theme	Random	80:85	arg1	Copolymer					87:95	PEG-Diisostearate Amphiphilic Random Copolymer	50:95	PEG-Diisostearate Amphiphilic Random Copolymer	50:95	Surface Morphology of Cosmetic Film Consisting of PEG-Diisostearate Amphiphilic Random Copolymer, Xanthan Gum, and Solvents.
29021489	6	59	dep	factors	1057:1063	arg1	miscibility					1070:1080	the miscibility	1066:1080	the miscibility (or the interfacial tension) of MPM-GDM against the solvents	1066:1141	It was assumed that the size of the MPM-GDM domain was determined by the balance between two factors: the miscibility (or the interfacial tension) of MPM-GDM against the solvents and the viscosity of the continuous phase.
29021489	6	59	dep	factors	1057:1063	arg1	viscosity					1151:1159	the viscosity	1147:1159	the viscosity of the continuous phase	1147:1183	It was assumed that the size of the MPM-GDM domain was determined by the balance between two factors: the miscibility (or the interfacial tension) of MPM-GDM against the solvents and the viscosity of the continuous phase.
29021489	6	59	dep	factors	1057:1063	arg1	factors					1057:1063	two factors	1053:1063	two factors: the miscibility (or the interfacial tension) of MPM-GDM against the solvents and the viscosity of the continuous phase	1053:1183	It was assumed that the size of the MPM-GDM domain was determined by the balance between two factors: the miscibility (or the interfacial tension) of MPM-GDM against the solvents and the viscosity of the continuous phase.
29021489	6	59	dep	factors	1057:1063	arg1	tension					1102:1108	the interfacial tension	1086:1108	the interfacial tension	1086:1108	It was assumed that the size of the MPM-GDM domain was determined by the balance between two factors: the miscibility (or the interfacial tension) of MPM-GDM against the solvents and the viscosity of the continuous phase.
29021489	5	60	theme	cosmetic	791:798	arg1	film					800:803	the cosmetic film	787:803	the cosmetic film	787:803	Confocal laser scanning microscopy (CLSM) images of the cosmetic film showed that MPM-GDM spread through the whole film in the absence of glycerin, whereas the addition of glycerin led to the formation of a sea-island structure.
29021489	3	61	theme	monohydric	513:522	arg1	alcohols					524:531	monohydric alcohols	513:531	monohydric alcohols	513:531	MPM-GDM was soluble in water, monohydric alcohols, and 1,3-butylene glycol (1,3-BG), whereas it hardly dissolved in glycerin.
29021489	1	62	theme	glycol-23	235:243	arg1	MPM-GDM					305:311	MPM-GDM	305:311	MPM-GDM	305:311	The surface morphology of a cosmetic film consisting of an amphiphilic random copolymer (methoxy polyethylene glycol-23 methacrylate/glyceryl diisostearate methacrylate copolymer, MPM-GDM) and solvents has been studied.
29021489	1	62	theme	glycol-23	235:243	arg1	copolymer					294:302	methoxy polyethylene glycol-23 methacrylate/glyceryl diisostearate methacrylate copolymer	214:302	methoxy polyethylene glycol-23 methacrylate/glyceryl diisostearate methacrylate copolymer	214:302	The surface morphology of a cosmetic film consisting of an amphiphilic random copolymer (methoxy polyethylene glycol-23 methacrylate/glyceryl diisostearate methacrylate copolymer, MPM-GDM) and solvents has been studied.
29021489	0	63	theme	Amphiphilic	68:78	arg1	Copolymer					87:95	PEG-Diisostearate Amphiphilic Random Copolymer	50:95	PEG-Diisostearate Amphiphilic Random Copolymer	50:95	Surface Morphology of Cosmetic Film Consisting of PEG-Diisostearate Amphiphilic Random Copolymer, Xanthan Gum, and Solvents.
29021489	5	64	theme	microscopy	759:768	arg1	images					777:782	Confocal laser scanning microscopy (CLSM) images	735:782	Confocal laser scanning microscopy (CLSM) images of the cosmetic film	735:803	Confocal laser scanning microscopy (CLSM) images of the cosmetic film showed that MPM-GDM spread through the whole film in the absence of glycerin, whereas the addition of glycerin led to the formation of a sea-island structure.
29021489	1	65	theme	methacrylate/glyceryl	245:265	arg1	MPM-GDM					305:311	MPM-GDM	305:311	MPM-GDM	305:311	The surface morphology of a cosmetic film consisting of an amphiphilic random copolymer (methoxy polyethylene glycol-23 methacrylate/glyceryl diisostearate methacrylate copolymer, MPM-GDM) and solvents has been studied.
29021489	1	65	theme	methacrylate/glyceryl	245:265	arg1	copolymer					294:302	methoxy polyethylene glycol-23 methacrylate/glyceryl diisostearate methacrylate copolymer	214:302	methoxy polyethylene glycol-23 methacrylate/glyceryl diisostearate methacrylate copolymer	214:302	The surface morphology of a cosmetic film consisting of an amphiphilic random copolymer (methoxy polyethylene glycol-23 methacrylate/glyceryl diisostearate methacrylate copolymer, MPM-GDM) and solvents has been studied.
26959542	1	0	theme	polyethylene	339:350	arg1	oxide					352:356	polyethylene oxide	339:356	polyethylene oxide (PEO) N12K	339:367	The aim of this research was to formulate effervescent floating drug delivery systems of theophylline using different release retarding polymers such as ethyl cellulose, Eudragit® L100, xanthan gum and polyethylene oxide (PEO) N12K.
26959542	1	0	theme	polyethylene	339:350	arg1	PEO					359:361	PEO	359:361	PEO	359:361	The aim of this research was to formulate effervescent floating drug delivery systems of theophylline using different release retarding polymers such as ethyl cellulose, Eudragit® L100, xanthan gum and polyethylene oxide (PEO) N12K.
26959542	2	1	used	used	393:396	arg2	agent					418:422	a gas generating agent	401:422	a gas generating agent	401:422	Sodium bicarbonate was used as a gas generating agent.
26959542	2	1	used	used	393:396	arg2	bicarbonate					377:387	Sodium bicarbonate	370:387	Sodium bicarbonate	370:387	Sodium bicarbonate was used as a gas generating agent.
26959542	1	2	theme	research	153:160	arg1	aim					141:143	The aim	137:143	The aim of this research	137:160	The aim of this research was to formulate effervescent floating drug delivery systems of theophylline using different release retarding polymers such as ethyl cellulose, Eudragit® L100, xanthan gum and polyethylene oxide (PEO) N12K.
26959542	1	3	theme	retarding	263:271	arg1	polymers					273:280	different release retarding polymers	245:280	different release retarding polymers such as ethyl cellulose, Eudragit® L100, xanthan gum and polyethylene oxide (PEO) N12K	245:367	The aim of this research was to formulate effervescent floating drug delivery systems of theophylline using different release retarding polymers such as ethyl cellulose, Eudragit® L100, xanthan gum and polyethylene oxide (PEO) N12K.
26959542	1	3	theme	retarding	263:271	arg1	N12K					364:367	polyethylene oxide (PEO) N12K	339:367	polyethylene oxide (PEO) N12K	339:367	The aim of this research was to formulate effervescent floating drug delivery systems of theophylline using different release retarding polymers such as ethyl cellulose, Eudragit® L100, xanthan gum and polyethylene oxide (PEO) N12K.
26959542	1	3	theme	retarding	263:271	arg1	cellulose					296:304	ethyl cellulose	290:304	ethyl cellulose	290:304	The aim of this research was to formulate effervescent floating drug delivery systems of theophylline using different release retarding polymers such as ethyl cellulose, Eudragit® L100, xanthan gum and polyethylene oxide (PEO) N12K.
26959542	1	3	theme	retarding	263:271	arg1	L100					317:320	Eudragit® L100	307:320	Eudragit® L100	307:320	The aim of this research was to formulate effervescent floating drug delivery systems of theophylline using different release retarding polymers such as ethyl cellulose, Eudragit® L100, xanthan gum and polyethylene oxide (PEO) N12K.
26959542	1	3	theme	retarding	263:271	arg1	gum					331:333	xanthan gum	323:333	xanthan gum	323:333	The aim of this research was to formulate effervescent floating drug delivery systems of theophylline using different release retarding polymers such as ethyl cellulose, Eudragit® L100, xanthan gum and polyethylene oxide (PEO) N12K.
26959542	0	4	theme	system	78:83	arg1	Design					0:5	Design	0:5	Design	0:5	Design and statistical optimization of an effervescent floating drug delivery system of theophylline using response surface methodology.
26959542	0	4	theme	system	78:83	arg1	optimization					23:34	statistical optimization	11:34	statistical optimization	11:34	Design and statistical optimization of an effervescent floating drug delivery system of theophylline using response surface methodology.
26959542	1	5	theme	oxide	352:356	arg1	N12K					364:367	polyethylene oxide (PEO) N12K	339:367	polyethylene oxide (PEO) N12K	339:367	The aim of this research was to formulate effervescent floating drug delivery systems of theophylline using different release retarding polymers such as ethyl cellulose, Eudragit® L100, xanthan gum and polyethylene oxide (PEO) N12K.
26959542	6	6	theme	drug	835:838	arg1	time					808:811	floating lag time	795:811	floating lag time	795:811	Further subjects of research were the effect of formulation variables on floating lag time and the percentage of drug released at the seventh hour (D7h).
26959542	6	6	theme	drug	835:838	arg1	percentage					821:830	the percentage	817:830	the percentage of drug	817:838	Further subjects of research were the effect of formulation variables on floating lag time and the percentage of drug released at the seventh hour (D7h).
26959542	8	7	theme	Scanning	1161:1168	arg1	DSC					1183:1185	DSC	1183:1185	DSC	1183:1185	No interaction was found between theophylline and PEO by Fourier Transformation Infrared spectroscopy (FTIR) and Differential Scanning Calorimetry (DSC) studies.
26959542	8	7	theme	Scanning	1161:1168	arg1	Calorimetry					1170:1180	Differential Scanning Calorimetry	1148:1180	Differential Scanning Calorimetry (DSC)	1148:1186	No interaction was found between theophylline and PEO by Fourier Transformation Infrared spectroscopy (FTIR) and Differential Scanning Calorimetry (DSC) studies.
26959542	6	8	theme	research	742:749	arg1	effect					760:765	the effect	756:765	the effect of formulation variables on floating lag time and the percentage of drug released at the seventh hour (D7h)	756:873	Further subjects of research were the effect of formulation variables on floating lag time and the percentage of drug released at the seventh hour (D7h).
26959542	6	8	theme	research	742:749	arg1	subjects					730:737	Further subjects	722:737	Further subjects of research	722:749	Further subjects of research were the effect of formulation variables on floating lag time and the percentage of drug released at the seventh hour (D7h).
26959542	4	9	theme	drug	612:615	arg1	properties					625:634	better drug release properties	605:634	better drug release properties	605:634	PEO based formulations produced better drug release properties than other formulations.
26959542	0	10	theme	theophylline	88:99	arg1	Design					0:5	Design	0:5	Design	0:5	Design and statistical optimization of an effervescent floating drug delivery system of theophylline using response surface methodology.
26959542	0	10	theme	theophylline	88:99	arg1	optimization					23:34	statistical optimization	11:34	statistical optimization	11:34	Design and statistical optimization of an effervescent floating drug delivery system of theophylline using response surface methodology.
26959542	3	11	theme	dissolution	544:554	arg1	characteristics					556:570	their physicochemical and dissolution characteristics	518:570	characteristics	556:570	Direct compression was used to formulate floating tablets and the tablets were evaluated for their physicochemical and dissolution characteristics.
26959542	4	12	theme	better	605:610	arg1	properties					625:634	better drug release properties	605:634	better drug release properties	605:634	PEO based formulations produced better drug release properties than other formulations.
26959542	0	13	theme	surface	116:122	arg1	methodology					124:134	response surface methodology	107:134	response surface methodology	107:134	Design and statistical optimization of an effervescent floating drug delivery system of theophylline using response surface methodology.
26959542	2	14	theme	gas	403:405	arg1	agent					418:422	a gas generating agent	401:422	a gas generating agent	401:422	Sodium bicarbonate was used as a gas generating agent.
26959542	2	14	theme	gas	403:405	arg1	bicarbonate					377:387	Sodium bicarbonate	370:387	Sodium bicarbonate	370:387	Sodium bicarbonate was used as a gas generating agent.
26959542	3	15	theme	Direct	425:430	arg1	compression					432:442	Direct compression	425:442	Direct compression	425:442	Direct compression was used to formulate floating tablets and the tablets were evaluated for their physicochemical and dissolution characteristics.
26959542	0	16	theme	response	107:114	arg1	methodology					124:134	response surface methodology	107:134	response surface methodology	107:134	Design and statistical optimization of an effervescent floating drug delivery system of theophylline using response surface methodology.
26959542	6	17	theme	Further	722:728	arg1	effect					760:765	the effect	756:765	the effect of formulation variables on floating lag time and the percentage of drug released at the seventh hour (D7h)	756:873	Further subjects of research were the effect of formulation variables on floating lag time and the percentage of drug released at the seventh hour (D7h).
26959542	6	17	theme	Further	722:728	arg1	subjects					730:737	Further subjects	722:737	Further subjects of research	722:749	Further subjects of research were the effect of formulation variables on floating lag time and the percentage of drug released at the seventh hour (D7h).
26959542	7	18	theme	optimized	1012:1020	arg1	quantities					888:897	The optimum quantities	876:897	The optimum quantities of PEO and sodium bicarbonate, which had the highest desirability close to 1.0,	876:977	The optimum quantities of PEO and sodium bicarbonate, which had the highest desirability close to 1.0, were chosen as the statistically optimized formulation.
26959542	7	18	theme	optimized	1012:1020	arg1	formulation					1022:1032	the statistically optimized formulation	994:1032	the statistically optimized formulation	994:1032	The optimum quantities of PEO and sodium bicarbonate, which had the highest desirability close to 1.0, were chosen as the statistically optimized formulation.
26959542	8	19	dep	spectroscopy	1124:1135	arg1	studies					1188:1194	studies	1188:1194	studies	1188:1194	No interaction was found between theophylline and PEO by Fourier Transformation Infrared spectroscopy (FTIR) and Differential Scanning Calorimetry (DSC) studies.
26959542	4	20	theme	PEO	573:575	arg1	formulations					583:594	PEO based formulations	573:594	PEO based formulations	573:594	PEO based formulations produced better drug release properties than other formulations.
26959542	3	21	used	used	448:451	arg2	compression					432:442	Direct compression	425:442	Direct compression	425:442	Direct compression was used to formulate floating tablets and the tablets were evaluated for their physicochemical and dissolution characteristics.
26959542	8	22	theme	Fourier	1092:1098	arg1	FTIR					1138:1141	FTIR	1138:1141	FTIR	1138:1141	No interaction was found between theophylline and PEO by Fourier Transformation Infrared spectroscopy (FTIR) and Differential Scanning Calorimetry (DSC) studies.
26959542	8	22	theme	Fourier	1092:1098	arg1	spectroscopy					1124:1135	Fourier Transformation Infrared spectroscopy	1092:1135	Fourier Transformation Infrared spectroscopy (FTIR)	1092:1142	No interaction was found between theophylline and PEO by Fourier Transformation Infrared spectroscopy (FTIR) and Differential Scanning Calorimetry (DSC) studies.
26959542	1	23	theme	effervescent	179:190	arg1	systems					215:221	effervescent floating drug delivery systems	179:221	effervescent floating drug delivery systems of theophylline using different release retarding polymers such as ethyl cellulose, Eudragit® L100, xanthan gum and polyethylene oxide (PEO) N12K	179:367	The aim of this research was to formulate effervescent floating drug delivery systems of theophylline using different release retarding polymers such as ethyl cellulose, Eudragit® L100, xanthan gum and polyethylene oxide (PEO) N12K.
26959542	1	24	theme	ethyl	290:294	arg1	cellulose					296:304	ethyl cellulose	290:304	ethyl cellulose	290:304	The aim of this research was to formulate effervescent floating drug delivery systems of theophylline using different release retarding polymers such as ethyl cellulose, Eudragit® L100, xanthan gum and polyethylene oxide (PEO) N12K.
26959542	6	25	theme	lag	804:806	arg1	time					808:811	floating lag time	795:811	floating lag time	795:811	Further subjects of research were the effect of formulation variables on floating lag time and the percentage of drug released at the seventh hour (D7h).
26959542	1	26	theme	floating	192:199	arg1	systems					215:221	effervescent floating drug delivery systems	179:221	effervescent floating drug delivery systems of theophylline using different release retarding polymers such as ethyl cellulose, Eudragit® L100, xanthan gum and polyethylene oxide (PEO) N12K	179:367	The aim of this research was to formulate effervescent floating drug delivery systems of theophylline using different release retarding polymers such as ethyl cellulose, Eudragit® L100, xanthan gum and polyethylene oxide (PEO) N12K.
26959542	7	27	contain	had	936:938	arg1	PEO					902:904	PEO	902:904	PEO	902:904	The optimum quantities of PEO and sodium bicarbonate, which had the highest desirability close to 1.0, were chosen as the statistically optimized formulation.
26959542	7	27	contain	had	936:938	arg1	bicarbonate					917:927	sodium bicarbonate	910:927	sodium bicarbonate	910:927	The optimum quantities of PEO and sodium bicarbonate, which had the highest desirability close to 1.0, were chosen as the statistically optimized formulation.
26959542	7	27	contain	had	936:938	arg2	desirability					952:963	the highest desirability	940:963	the highest desirability close to 1.0	940:976	The optimum quantities of PEO and sodium bicarbonate, which had the highest desirability close to 1.0, were chosen as the statistically optimized formulation.
26959542	7	28	theme	bicarbonate	917:927	arg1	quantities					888:897	The optimum quantities	876:897	The optimum quantities of PEO and sodium bicarbonate, which had the highest desirability close to 1.0,	876:977	The optimum quantities of PEO and sodium bicarbonate, which had the highest desirability close to 1.0, were chosen as the statistically optimized formulation.
26959542	7	28	theme	bicarbonate	917:927	arg1	formulation					1022:1032	the statistically optimized formulation	994:1032	the statistically optimized formulation	994:1032	The optimum quantities of PEO and sodium bicarbonate, which had the highest desirability close to 1.0, were chosen as the statistically optimized formulation.
26959542	6	29	theme	floating	795:802	arg1	time					808:811	floating lag time	795:811	floating lag time	795:811	Further subjects of research were the effect of formulation variables on floating lag time and the percentage of drug released at the seventh hour (D7h).
26959542	1	30	theme	drug	201:204	arg1	systems					215:221	effervescent floating drug delivery systems	179:221	effervescent floating drug delivery systems of theophylline using different release retarding polymers such as ethyl cellulose, Eudragit® L100, xanthan gum and polyethylene oxide (PEO) N12K	179:367	The aim of this research was to formulate effervescent floating drug delivery systems of theophylline using different release retarding polymers such as ethyl cellulose, Eudragit® L100, xanthan gum and polyethylene oxide (PEO) N12K.
26959542	0	31	theme	statistical	11:21	arg1	optimization					23:34	statistical optimization	11:34	statistical optimization	11:34	Design and statistical optimization of an effervescent floating drug delivery system of theophylline using response surface methodology.
26959542	4	32	theme	based	577:581	arg1	formulations					583:594	PEO based formulations	573:594	PEO based formulations	573:594	PEO based formulations produced better drug release properties than other formulations.
26959542	7	33	theme	highest	944:950	arg1	desirability					952:963	the highest desirability	940:963	the highest desirability close to 1.0	940:976	The optimum quantities of PEO and sodium bicarbonate, which had the highest desirability close to 1.0, were chosen as the statistically optimized formulation.
26959542	1	34	theme	delivery	206:213	arg1	systems					215:221	effervescent floating drug delivery systems	179:221	effervescent floating drug delivery systems of theophylline using different release retarding polymers such as ethyl cellulose, Eudragit® L100, xanthan gum and polyethylene oxide (PEO) N12K	179:367	The aim of this research was to formulate effervescent floating drug delivery systems of theophylline using different release retarding polymers such as ethyl cellulose, Eudragit® L100, xanthan gum and polyethylene oxide (PEO) N12K.
26959542	1	35	theme	Eudragit®	307:315	arg1	L100					317:320	Eudragit® L100	307:320	Eudragit® L100	307:320	The aim of this research was to formulate effervescent floating drug delivery systems of theophylline using different release retarding polymers such as ethyl cellulose, Eudragit® L100, xanthan gum and polyethylene oxide (PEO) N12K.
26959542	2	36	theme	Sodium	370:375	arg1	agent					418:422	a gas generating agent	401:422	a gas generating agent	401:422	Sodium bicarbonate was used as a gas generating agent.
26959542	2	36	theme	Sodium	370:375	arg1	bicarbonate					377:387	Sodium bicarbonate	370:387	Sodium bicarbonate	370:387	Sodium bicarbonate was used as a gas generating agent.
26959542	6	37	from	effect	760:765	arg1	time					808:811	floating lag time	795:811	floating lag time	795:811	Further subjects of research were the effect of formulation variables on floating lag time and the percentage of drug released at the seventh hour (D7h).
26959542	6	37	from	effect	760:765	arg1	percentage					821:830	the percentage	817:830	the percentage of drug	817:838	Further subjects of research were the effect of formulation variables on floating lag time and the percentage of drug released at the seventh hour (D7h).
26959542	4	38	theme	other	641:645	arg1	formulations					647:658	other formulations	641:658	other formulations	641:658	PEO based formulations produced better drug release properties than other formulations.
26959542	0	39	theme	floating	55:62	arg1	system					78:83	an effervescent floating drug delivery system	39:83	an effervescent floating drug delivery system	39:83	Design and statistical optimization of an effervescent floating drug delivery system of theophylline using response surface methodology.
26959542	8	40	theme	Transformation	1100:1113	arg1	FTIR					1138:1141	FTIR	1138:1141	FTIR	1138:1141	No interaction was found between theophylline and PEO by Fourier Transformation Infrared spectroscopy (FTIR) and Differential Scanning Calorimetry (DSC) studies.
26959542	8	40	theme	Transformation	1100:1113	arg1	spectroscopy					1124:1135	Fourier Transformation Infrared spectroscopy	1092:1135	Fourier Transformation Infrared spectroscopy (FTIR)	1092:1142	No interaction was found between theophylline and PEO by Fourier Transformation Infrared spectroscopy (FTIR) and Differential Scanning Calorimetry (DSC) studies.
26959542	6	41	theme	variables	782:790	arg1	effect					760:765	the effect	756:765	the effect of formulation variables on floating lag time and the percentage of drug released at the seventh hour (D7h)	756:873	Further subjects of research were the effect of formulation variables on floating lag time and the percentage of drug released at the seventh hour (D7h).
26959542	6	41	theme	variables	782:790	arg1	subjects					730:737	Further subjects	722:737	Further subjects of research	722:749	Further subjects of research were the effect of formulation variables on floating lag time and the percentage of drug released at the seventh hour (D7h).
26959542	0	42	theme	effervescent	42:53	arg1	system					78:83	an effervescent floating drug delivery system	39:83	an effervescent floating drug delivery system	39:83	Design and statistical optimization of an effervescent floating drug delivery system of theophylline using response surface methodology.
26959542	1	43	theme	xanthan	323:329	arg1	gum					331:333	xanthan gum	323:333	xanthan gum	323:333	The aim of this research was to formulate effervescent floating drug delivery systems of theophylline using different release retarding polymers such as ethyl cellulose, Eudragit® L100, xanthan gum and polyethylene oxide (PEO) N12K.
26959542	5	44	theme	central	696:702	arg1	design					714:719	central composite design	696:719	central composite design	696:719	Hence, it was further optimized by central composite design.
26959542	6	45	theme	seventh	856:862	arg1	D7h					870:872	D7h	870:872	D7h	870:872	Further subjects of research were the effect of formulation variables on floating lag time and the percentage of drug released at the seventh hour (D7h).
26959542	6	45	theme	seventh	856:862	arg1	hour					864:867	the seventh hour	852:867	the seventh hour (D7h)	852:873	Further subjects of research were the effect of formulation variables on floating lag time and the percentage of drug released at the seventh hour (D7h).
26959542	6	46	theme	formulation	770:780	arg1	variables					782:790	formulation variables	770:790	formulation variables	770:790	Further subjects of research were the effect of formulation variables on floating lag time and the percentage of drug released at the seventh hour (D7h).
26959542	5	47	theme	composite	704:712	arg1	design					714:719	central composite design	696:719	central composite design	696:719	Hence, it was further optimized by central composite design.
26959542	8	48	theme	Differential	1148:1159	arg1	DSC					1183:1185	DSC	1183:1185	DSC	1183:1185	No interaction was found between theophylline and PEO by Fourier Transformation Infrared spectroscopy (FTIR) and Differential Scanning Calorimetry (DSC) studies.
26959542	8	48	theme	Differential	1148:1159	arg1	Calorimetry					1170:1180	Differential Scanning Calorimetry	1148:1180	Differential Scanning Calorimetry (DSC)	1148:1186	No interaction was found between theophylline and PEO by Fourier Transformation Infrared spectroscopy (FTIR) and Differential Scanning Calorimetry (DSC) studies.
26959542	3	49	theme	floating	466:473	arg1	tablets					475:481	floating tablets	466:481	floating tablets	466:481	Direct compression was used to formulate floating tablets and the tablets were evaluated for their physicochemical and dissolution characteristics.
26959542	7	50	theme	sodium	910:915	arg1	bicarbonate					917:927	sodium bicarbonate	910:927	sodium bicarbonate	910:927	The optimum quantities of PEO and sodium bicarbonate, which had the highest desirability close to 1.0, were chosen as the statistically optimized formulation.
26959542	1	51	theme	theophylline	226:237	arg1	systems					215:221	effervescent floating drug delivery systems	179:221	effervescent floating drug delivery systems of theophylline using different release retarding polymers such as ethyl cellulose, Eudragit® L100, xanthan gum and polyethylene oxide (PEO) N12K	179:367	The aim of this research was to formulate effervescent floating drug delivery systems of theophylline using different release retarding polymers such as ethyl cellulose, Eudragit® L100, xanthan gum and polyethylene oxide (PEO) N12K.
26959542	8	52	theme	Infrared	1115:1122	arg1	FTIR					1138:1141	FTIR	1138:1141	FTIR	1138:1141	No interaction was found between theophylline and PEO by Fourier Transformation Infrared spectroscopy (FTIR) and Differential Scanning Calorimetry (DSC) studies.
26959542	8	52	theme	Infrared	1115:1122	arg1	spectroscopy					1124:1135	Fourier Transformation Infrared spectroscopy	1092:1135	Fourier Transformation Infrared spectroscopy (FTIR)	1092:1142	No interaction was found between theophylline and PEO by Fourier Transformation Infrared spectroscopy (FTIR) and Differential Scanning Calorimetry (DSC) studies.
26959542	4	53	theme	release	617:623	arg1	properties					625:634	better drug release properties	605:634	better drug release properties	605:634	PEO based formulations produced better drug release properties than other formulations.
26959542	0	54	theme	delivery	69:76	arg1	system					78:83	an effervescent floating drug delivery system	39:83	an effervescent floating drug delivery system	39:83	Design and statistical optimization of an effervescent floating drug delivery system of theophylline using response surface methodology.
26959542	2	55	theme	generating	407:416	arg1	agent					418:422	a gas generating agent	401:422	a gas generating agent	401:422	Sodium bicarbonate was used as a gas generating agent.
26959542	2	55	theme	generating	407:416	arg1	bicarbonate					377:387	Sodium bicarbonate	370:387	Sodium bicarbonate	370:387	Sodium bicarbonate was used as a gas generating agent.
26959542	7	56	theme	PEO	902:904	arg1	quantities					888:897	The optimum quantities	876:897	The optimum quantities of PEO and sodium bicarbonate, which had the highest desirability close to 1.0,	876:977	The optimum quantities of PEO and sodium bicarbonate, which had the highest desirability close to 1.0, were chosen as the statistically optimized formulation.
26959542	7	56	theme	PEO	902:904	arg1	formulation					1022:1032	the statistically optimized formulation	994:1032	the statistically optimized formulation	994:1032	The optimum quantities of PEO and sodium bicarbonate, which had the highest desirability close to 1.0, were chosen as the statistically optimized formulation.
26959542	1	57	theme	different	245:253	arg1	polymers					273:280	different release retarding polymers	245:280	different release retarding polymers such as ethyl cellulose, Eudragit® L100, xanthan gum and polyethylene oxide (PEO) N12K	245:367	The aim of this research was to formulate effervescent floating drug delivery systems of theophylline using different release retarding polymers such as ethyl cellulose, Eudragit® L100, xanthan gum and polyethylene oxide (PEO) N12K.
26959542	1	57	theme	different	245:253	arg1	N12K					364:367	polyethylene oxide (PEO) N12K	339:367	polyethylene oxide (PEO) N12K	339:367	The aim of this research was to formulate effervescent floating drug delivery systems of theophylline using different release retarding polymers such as ethyl cellulose, Eudragit® L100, xanthan gum and polyethylene oxide (PEO) N12K.
26959542	1	57	theme	different	245:253	arg1	cellulose					296:304	ethyl cellulose	290:304	ethyl cellulose	290:304	The aim of this research was to formulate effervescent floating drug delivery systems of theophylline using different release retarding polymers such as ethyl cellulose, Eudragit® L100, xanthan gum and polyethylene oxide (PEO) N12K.
26959542	1	57	theme	different	245:253	arg1	L100					317:320	Eudragit® L100	307:320	Eudragit® L100	307:320	The aim of this research was to formulate effervescent floating drug delivery systems of theophylline using different release retarding polymers such as ethyl cellulose, Eudragit® L100, xanthan gum and polyethylene oxide (PEO) N12K.
26959542	1	57	theme	different	245:253	arg1	gum					331:333	xanthan gum	323:333	xanthan gum	323:333	The aim of this research was to formulate effervescent floating drug delivery systems of theophylline using different release retarding polymers such as ethyl cellulose, Eudragit® L100, xanthan gum and polyethylene oxide (PEO) N12K.
26959542	0	58	theme	drug	64:67	arg1	system					78:83	an effervescent floating drug delivery system	39:83	an effervescent floating drug delivery system	39:83	Design and statistical optimization of an effervescent floating drug delivery system of theophylline using response surface methodology.
26959542	7	59	theme	optimum	880:886	arg1	quantities					888:897	The optimum quantities	876:897	The optimum quantities of PEO and sodium bicarbonate, which had the highest desirability close to 1.0,	876:977	The optimum quantities of PEO and sodium bicarbonate, which had the highest desirability close to 1.0, were chosen as the statistically optimized formulation.
26959542	7	59	theme	optimum	880:886	arg1	formulation					1022:1032	the statistically optimized formulation	994:1032	the statistically optimized formulation	994:1032	The optimum quantities of PEO and sodium bicarbonate, which had the highest desirability close to 1.0, were chosen as the statistically optimized formulation.
26959542	1	60	theme	release	255:261	arg1	polymers					273:280	different release retarding polymers	245:280	different release retarding polymers such as ethyl cellulose, Eudragit® L100, xanthan gum and polyethylene oxide (PEO) N12K	245:367	The aim of this research was to formulate effervescent floating drug delivery systems of theophylline using different release retarding polymers such as ethyl cellulose, Eudragit® L100, xanthan gum and polyethylene oxide (PEO) N12K.
26959542	1	60	theme	release	255:261	arg1	N12K					364:367	polyethylene oxide (PEO) N12K	339:367	polyethylene oxide (PEO) N12K	339:367	The aim of this research was to formulate effervescent floating drug delivery systems of theophylline using different release retarding polymers such as ethyl cellulose, Eudragit® L100, xanthan gum and polyethylene oxide (PEO) N12K.
26959542	1	60	theme	release	255:261	arg1	cellulose					296:304	ethyl cellulose	290:304	ethyl cellulose	290:304	The aim of this research was to formulate effervescent floating drug delivery systems of theophylline using different release retarding polymers such as ethyl cellulose, Eudragit® L100, xanthan gum and polyethylene oxide (PEO) N12K.
26959542	1	60	theme	release	255:261	arg1	L100					317:320	Eudragit® L100	307:320	Eudragit® L100	307:320	The aim of this research was to formulate effervescent floating drug delivery systems of theophylline using different release retarding polymers such as ethyl cellulose, Eudragit® L100, xanthan gum and polyethylene oxide (PEO) N12K.
26959542	1	60	theme	release	255:261	arg1	gum					331:333	xanthan gum	323:333	xanthan gum	323:333	The aim of this research was to formulate effervescent floating drug delivery systems of theophylline using different release retarding polymers such as ethyl cellulose, Eudragit® L100, xanthan gum and polyethylene oxide (PEO) N12K.
27112888	2	0	theme	CAT	504:506	arg1	activities					485:494	the renal activities	475:494	the renal activities of SOD, CAT and GSH-Px	475:517	Biochemical and antioxidant analysis showed that the En-RPS had potential effects in decreasing the serum levels of CRE, BUN, ALB and GLU significantly, increasing the renal activities of SOD, CAT and GSH-Px remarkably, and reducing the renal contents of MDA prominently.
27112888	4	1	theme	nephropathy	906:916	arg1	progression					882:892	the progression	878:892	the progression of diabetic nephropathy	878:916	These results demonstrated that En-RPS extracted from the residue of F. velutipes possessed potent antioxidant activities, and could be used as a promising therapeutic agent for inhibiting the progression of diabetic nephropathy.
27112888	2	2	theme	MDA	566:568	arg1	contents					554:561	the renal contents	544:561	the renal contents of MDA	544:568	Biochemical and antioxidant analysis showed that the En-RPS had potential effects in decreasing the serum levels of CRE, BUN, ALB and GLU significantly, increasing the renal activities of SOD, CAT and GSH-Px remarkably, and reducing the renal contents of MDA prominently.
27112888	4	3	theme	diabetic	897:904	arg1	nephropathy					906:916	diabetic nephropathy	897:916	diabetic nephropathy	897:916	These results demonstrated that En-RPS extracted from the residue of F. velutipes possessed potent antioxidant activities, and could be used as a promising therapeutic agent for inhibiting the progression of diabetic nephropathy.
27112888	5	4	theme	monosaccharide	936:949	arg1	compositions					951:962	the monosaccharide compositions	932:962	the monosaccharide compositions of these three RPS	932:981	In addition, the monosaccharide compositions of these three RPS were also analyzed.
27112888	5	5	theme	RPS	979:981	arg1	compositions					951:962	the monosaccharide compositions	932:962	the monosaccharide compositions of these three RPS	932:981	In addition, the monosaccharide compositions of these three RPS were also analyzed.
27112888	2	6	theme	antioxidant	327:337	arg1	analysis					339:346	Biochemical and antioxidant analysis	311:346	Biochemical and antioxidant analysis	311:346	Biochemical and antioxidant analysis showed that the En-RPS had potential effects in decreasing the serum levels of CRE, BUN, ALB and GLU significantly, increasing the renal activities of SOD, CAT and GSH-Px remarkably, and reducing the renal contents of MDA prominently.
27112888	2	7	theme	CRE	427:429	arg1	levels					417:422	the serum levels	407:422	the serum levels of CRE, BUN, ALB and GLU	407:447	Biochemical and antioxidant analysis showed that the En-RPS had potential effects in decreasing the serum levels of CRE, BUN, ALB and GLU significantly, increasing the renal activities of SOD, CAT and GSH-Px remarkably, and reducing the renal contents of MDA prominently.
27112888	2	8	theme	BUN	432:434	arg1	levels					417:422	the serum levels	407:422	the serum levels of CRE, BUN, ALB and GLU	407:447	Biochemical and antioxidant analysis showed that the En-RPS had potential effects in decreasing the serum levels of CRE, BUN, ALB and GLU significantly, increasing the renal activities of SOD, CAT and GSH-Px remarkably, and reducing the renal contents of MDA prominently.
27112888	2	9	theme	renal	479:483	arg1	activities					485:494	the renal activities	475:494	the renal activities of SOD, CAT and GSH-Px	475:517	Biochemical and antioxidant analysis showed that the En-RPS had potential effects in decreasing the serum levels of CRE, BUN, ALB and GLU significantly, increasing the renal activities of SOD, CAT and GSH-Px remarkably, and reducing the renal contents of MDA prominently.
27112888	0	10	theme	residue	44:50	arg1	polysaccharides					52:66	residue polysaccharides	44:66	residue polysaccharides from Flammulina velutipes	44:92	Antioxidative and renoprotective effects of residue polysaccharides from Flammulina velutipes.
27112888	2	11	theme	Biochemical	311:321	arg1	analysis					339:346	Biochemical and antioxidant analysis	311:346	Biochemical and antioxidant analysis	311:346	Biochemical and antioxidant analysis showed that the En-RPS had potential effects in decreasing the serum levels of CRE, BUN, ALB and GLU significantly, increasing the renal activities of SOD, CAT and GSH-Px remarkably, and reducing the renal contents of MDA prominently.
27112888	1	12	theme	extractable	101:111	arg1	polysaccharides					113:127	Three extractable polysaccharides	95:127	Three extractable polysaccharides including Ac-RPS, Al-RPS and En-RPS	95:163	Three extractable polysaccharides including Ac-RPS, Al-RPS and En-RPS were extracted from the residue of Flammulina velutipes and their antioxidative and renoprotective effects on STZ-induced mice were investigated.
27112888	1	12	theme	extractable	101:111	arg1	En-RPS					158:163	En-RPS	158:163	En-RPS	158:163	Three extractable polysaccharides including Ac-RPS, Al-RPS and En-RPS were extracted from the residue of Flammulina velutipes and their antioxidative and renoprotective effects on STZ-induced mice were investigated.
27112888	1	12	theme	extractable	101:111	arg1	Al-RPS					147:152	Al-RPS	147:152	Al-RPS	147:152	Three extractable polysaccharides including Ac-RPS, Al-RPS and En-RPS were extracted from the residue of Flammulina velutipes and their antioxidative and renoprotective effects on STZ-induced mice were investigated.
27112888	1	12	theme	extractable	101:111	arg1	Ac-RPS					139:144	Ac-RPS	139:144	Ac-RPS	139:144	Three extractable polysaccharides including Ac-RPS, Al-RPS and En-RPS were extracted from the residue of Flammulina velutipes and their antioxidative and renoprotective effects on STZ-induced mice were investigated.
27112888	1	13	theme	Flammulina	200:209	arg1	velutipes					211:219	Flammulina velutipes	200:219	Flammulina velutipes	200:219	Three extractable polysaccharides including Ac-RPS, Al-RPS and En-RPS were extracted from the residue of Flammulina velutipes and their antioxidative and renoprotective effects on STZ-induced mice were investigated.
27112888	0	14	theme	Antioxidative	0:12	arg1	effects					33:39	Antioxidative and renoprotective effects	0:39	Antioxidative and renoprotective effects of residue polysaccharides from Flammulina velutipes	0:92	Antioxidative and renoprotective effects of residue polysaccharides from Flammulina velutipes.
27112888	4	15	theme	velutipes	761:769	arg1	residue					747:753	the residue	743:753	the residue of F. velutipes	743:769	These results demonstrated that En-RPS extracted from the residue of F. velutipes possessed potent antioxidant activities, and could be used as a promising therapeutic agent for inhibiting the progression of diabetic nephropathy.
27112888	4	15	theme	velutipes	761:769	arg1	velutipes					761:769	F. velutipes	758:769	F. velutipes	758:769	These results demonstrated that En-RPS extracted from the residue of F. velutipes possessed potent antioxidant activities, and could be used as a promising therapeutic agent for inhibiting the progression of diabetic nephropathy.
27112888	1	16	theme	velutipes	211:219	arg1	residue					189:195	the residue	185:195	the residue of Flammulina velutipes	185:219	Three extractable polysaccharides including Ac-RPS, Al-RPS and En-RPS were extracted from the residue of Flammulina velutipes and their antioxidative and renoprotective effects on STZ-induced mice were investigated.
27112888	1	16	theme	velutipes	211:219	arg1	velutipes					211:219	Flammulina velutipes	200:219	Flammulina velutipes	200:219	Three extractable polysaccharides including Ac-RPS, Al-RPS and En-RPS were extracted from the residue of Flammulina velutipes and their antioxidative and renoprotective effects on STZ-induced mice were investigated.
27112888	4	17	theme	antioxidant	788:798	arg1	activities					800:809	potent antioxidant activities	781:809	potent antioxidant activities	781:809	These results demonstrated that En-RPS extracted from the residue of F. velutipes possessed potent antioxidant activities, and could be used as a promising therapeutic agent for inhibiting the progression of diabetic nephropathy.
27112888	4	18	theme	therapeutic	845:855	arg1	agent					857:861	a promising therapeutic agent	833:861	a promising therapeutic agent for inhibiting the progression of diabetic nephropathy	833:916	These results demonstrated that En-RPS extracted from the residue of F. velutipes possessed potent antioxidant activities, and could be used as a promising therapeutic agent for inhibiting the progression of diabetic nephropathy.
27112888	4	18	theme	therapeutic	845:855	arg1	En-RPS					721:726	En-RPS	721:726	En-RPS extracted from the residue of F. velutipes	721:769	These results demonstrated that En-RPS extracted from the residue of F. velutipes possessed potent antioxidant activities, and could be used as a promising therapeutic agent for inhibiting the progression of diabetic nephropathy.
27112888	2	19	theme	GLU	445:447	arg1	levels					417:422	the serum levels	407:422	the serum levels of CRE, BUN, ALB and GLU	407:447	Biochemical and antioxidant analysis showed that the En-RPS had potential effects in decreasing the serum levels of CRE, BUN, ALB and GLU significantly, increasing the renal activities of SOD, CAT and GSH-Px remarkably, and reducing the renal contents of MDA prominently.
27112888	0	20	theme	renoprotective	18:31	arg1	effects					33:39	Antioxidative and renoprotective effects	0:39	Antioxidative and renoprotective effects of residue polysaccharides from Flammulina velutipes	0:92	Antioxidative and renoprotective effects of residue polysaccharides from Flammulina velutipes.
27112888	3	21	theme	kidney	674:679	arg1	damage					681:686	kidney damage	674:686	kidney damage	674:686	Furthermore, the histopathological observations also displayed that En-RPS could alleviate kidney damage.
27112888	4	22	theme	potent	781:786	arg1	activities					800:809	potent antioxidant activities	781:809	potent antioxidant activities	781:809	These results demonstrated that En-RPS extracted from the residue of F. velutipes possessed potent antioxidant activities, and could be used as a promising therapeutic agent for inhibiting the progression of diabetic nephropathy.
27112888	2	23	theme	SOD	499:501	arg1	activities					485:494	the renal activities	475:494	the renal activities of SOD, CAT and GSH-Px	475:517	Biochemical and antioxidant analysis showed that the En-RPS had potential effects in decreasing the serum levels of CRE, BUN, ALB and GLU significantly, increasing the renal activities of SOD, CAT and GSH-Px remarkably, and reducing the renal contents of MDA prominently.
27112888	4	24	contain	possessed	771:779	arg1	agent					857:861	a promising therapeutic agent	833:861	a promising therapeutic agent for inhibiting the progression of diabetic nephropathy	833:916	These results demonstrated that En-RPS extracted from the residue of F. velutipes possessed potent antioxidant activities, and could be used as a promising therapeutic agent for inhibiting the progression of diabetic nephropathy.
27112888	4	24	contain	possessed	771:779	arg1	En-RPS					721:726	En-RPS	721:726	En-RPS extracted from the residue of F. velutipes	721:769	These results demonstrated that En-RPS extracted from the residue of F. velutipes possessed potent antioxidant activities, and could be used as a promising therapeutic agent for inhibiting the progression of diabetic nephropathy.
27112888	4	24	contain	possessed	771:779	arg2	activities					800:809	potent antioxidant activities	781:809	potent antioxidant activities	781:809	These results demonstrated that En-RPS extracted from the residue of F. velutipes possessed potent antioxidant activities, and could be used as a promising therapeutic agent for inhibiting the progression of diabetic nephropathy.
27112888	2	25	theme	renal	548:552	arg1	contents					554:561	the renal contents	544:561	the renal contents of MDA	544:568	Biochemical and antioxidant analysis showed that the En-RPS had potential effects in decreasing the serum levels of CRE, BUN, ALB and GLU significantly, increasing the renal activities of SOD, CAT and GSH-Px remarkably, and reducing the renal contents of MDA prominently.
27112888	1	26	theme	antioxidative	231:243	arg1	effects					264:270	their antioxidative and renoprotective effects	225:270	their antioxidative and renoprotective effects on STZ-induced mice	225:290	Three extractable polysaccharides including Ac-RPS, Al-RPS and En-RPS were extracted from the residue of Flammulina velutipes and their antioxidative and renoprotective effects on STZ-induced mice were investigated.
27112888	3	27	theme	histopathological	600:616	arg1	observations					618:629	the histopathological observations	596:629	the histopathological observations	596:629	Furthermore, the histopathological observations also displayed that En-RPS could alleviate kidney damage.
27112888	4	28	used	used	825:828	arg2	agent					857:861	a promising therapeutic agent	833:861	a promising therapeutic agent for inhibiting the progression of diabetic nephropathy	833:916	These results demonstrated that En-RPS extracted from the residue of F. velutipes possessed potent antioxidant activities, and could be used as a promising therapeutic agent for inhibiting the progression of diabetic nephropathy.
27112888	4	28	used	used	825:828	arg2	En-RPS					721:726	En-RPS	721:726	En-RPS extracted from the residue of F. velutipes	721:769	These results demonstrated that En-RPS extracted from the residue of F. velutipes possessed potent antioxidant activities, and could be used as a promising therapeutic agent for inhibiting the progression of diabetic nephropathy.
27112888	2	29	theme	ALB	437:439	arg1	levels					417:422	the serum levels	407:422	the serum levels of CRE, BUN, ALB and GLU	407:447	Biochemical and antioxidant analysis showed that the En-RPS had potential effects in decreasing the serum levels of CRE, BUN, ALB and GLU significantly, increasing the renal activities of SOD, CAT and GSH-Px remarkably, and reducing the renal contents of MDA prominently.
27112888	0	30	from	velutipes	84:92	arg1	effects					33:39	Antioxidative and renoprotective effects	0:39	Antioxidative and renoprotective effects of residue polysaccharides from Flammulina velutipes	0:92	Antioxidative and renoprotective effects of residue polysaccharides from Flammulina velutipes.
27112888	0	30	from	velutipes	84:92	arg1	polysaccharides					52:66	residue polysaccharides	44:66	residue polysaccharides from Flammulina velutipes	44:92	Antioxidative and renoprotective effects of residue polysaccharides from Flammulina velutipes.
27112888	4	31	theme	promising	835:843	arg1	agent					857:861	a promising therapeutic agent	833:861	a promising therapeutic agent for inhibiting the progression of diabetic nephropathy	833:916	These results demonstrated that En-RPS extracted from the residue of F. velutipes possessed potent antioxidant activities, and could be used as a promising therapeutic agent for inhibiting the progression of diabetic nephropathy.
27112888	4	31	theme	promising	835:843	arg1	En-RPS					721:726	En-RPS	721:726	En-RPS extracted from the residue of F. velutipes	721:769	These results demonstrated that En-RPS extracted from the residue of F. velutipes possessed potent antioxidant activities, and could be used as a promising therapeutic agent for inhibiting the progression of diabetic nephropathy.
27112888	0	32	theme	polysaccharides	52:66	arg1	effects					33:39	Antioxidative and renoprotective effects	0:39	Antioxidative and renoprotective effects of residue polysaccharides from Flammulina velutipes	0:92	Antioxidative and renoprotective effects of residue polysaccharides from Flammulina velutipes.
27112888	2	33	contain	had	371:373	arg2	effects					385:391	potential effects	375:391	potential effects	375:391	Biochemical and antioxidant analysis showed that the En-RPS had potential effects in decreasing the serum levels of CRE, BUN, ALB and GLU significantly, increasing the renal activities of SOD, CAT and GSH-Px remarkably, and reducing the renal contents of MDA prominently.
27112888	2	33	contain	had	371:373	arg1	En-RPS					364:369	the En-RPS	360:369	the En-RPS	360:369	Biochemical and antioxidant analysis showed that the En-RPS had potential effects in decreasing the serum levels of CRE, BUN, ALB and GLU significantly, increasing the renal activities of SOD, CAT and GSH-Px remarkably, and reducing the renal contents of MDA prominently.
27112888	2	34	theme	potential	375:383	arg1	effects					385:391	potential effects	375:391	potential effects	375:391	Biochemical and antioxidant analysis showed that the En-RPS had potential effects in decreasing the serum levels of CRE, BUN, ALB and GLU significantly, increasing the renal activities of SOD, CAT and GSH-Px remarkably, and reducing the renal contents of MDA prominently.
27112888	1	35	theme	renoprotective	249:262	arg1	effects					264:270	their antioxidative and renoprotective effects	225:270	their antioxidative and renoprotective effects on STZ-induced mice	225:290	Three extractable polysaccharides including Ac-RPS, Al-RPS and En-RPS were extracted from the residue of Flammulina velutipes and their antioxidative and renoprotective effects on STZ-induced mice were investigated.
27112888	1	36	from	effects	264:270	arg1	mice					287:290	STZ-induced mice	275:290	STZ-induced mice	275:290	Three extractable polysaccharides including Ac-RPS, Al-RPS and En-RPS were extracted from the residue of Flammulina velutipes and their antioxidative and renoprotective effects on STZ-induced mice were investigated.
27112888	4	37	theme	F.	758:759	arg1	velutipes					761:769	F. velutipes	758:769	F. velutipes	758:769	These results demonstrated that En-RPS extracted from the residue of F. velutipes possessed potent antioxidant activities, and could be used as a promising therapeutic agent for inhibiting the progression of diabetic nephropathy.
27112888	2	38	theme	GSH-Px	512:517	arg1	activities					485:494	the renal activities	475:494	the renal activities of SOD, CAT and GSH-Px	475:517	Biochemical and antioxidant analysis showed that the En-RPS had potential effects in decreasing the serum levels of CRE, BUN, ALB and GLU significantly, increasing the renal activities of SOD, CAT and GSH-Px remarkably, and reducing the renal contents of MDA prominently.
27112888	0	39	theme	Flammulina	73:82	arg1	velutipes					84:92	Flammulina velutipes	73:92	Flammulina velutipes	73:92	Antioxidative and renoprotective effects of residue polysaccharides from Flammulina velutipes.
27112888	0	40	from	effects	33:39	arg1	velutipes					84:92	Flammulina velutipes	73:92	Flammulina velutipes	73:92	Antioxidative and renoprotective effects of residue polysaccharides from Flammulina velutipes.
27112888	2	41	theme	serum	411:415	arg1	levels					417:422	the serum levels	407:422	the serum levels of CRE, BUN, ALB and GLU	407:447	Biochemical and antioxidant analysis showed that the En-RPS had potential effects in decreasing the serum levels of CRE, BUN, ALB and GLU significantly, increasing the renal activities of SOD, CAT and GSH-Px remarkably, and reducing the renal contents of MDA prominently.
27112888	1	42	theme	STZ-induced	275:285	arg1	mice					287:290	STZ-induced mice	275:290	STZ-induced mice	275:290	Three extractable polysaccharides including Ac-RPS, Al-RPS and En-RPS were extracted from the residue of Flammulina velutipes and their antioxidative and renoprotective effects on STZ-induced mice were investigated.
28684354	5	0	theme	flax	579:582	arg1	fiber					584:588	flax fiber	579:588	flax fiber	579:588	CNC was isolated from flax fiber using acid hydrolysis method.
28684354	3	1	theme	chitosan-based	465:478	arg1	films					480:484	chitosan-based films	465:484	chitosan-based films	465:484	The current study was conducted to check the effect of flax (Linum usitatissimum) cellulose nanocrystals (CNC) on mechanical and barrier properties of chitosan-based films.
28684354	0	2	theme	reinforcing	68:78	arg1	material					80:87	reinforcing material	68:87	reinforcing material	68:87	Utilization of flax (Linum usitatissimum) cellulose nanocrystals as reinforcing material for chitosan films.
28684354	8	3	theme	lower	875:879	arg1	crystallinity					886:898	overall lower film crystallinity	867:898	overall lower film crystallinity	867:898	No significant improvement was recorded in water vapor permeability due to overall lower film crystallinity.
28684354	3	4	theme	films	480:484	arg1	properties					451:460	mechanical and barrier properties	428:460	properties	451:460	The current study was conducted to check the effect of flax (Linum usitatissimum) cellulose nanocrystals (CNC) on mechanical and barrier properties of chitosan-based films.
28684354	7	5	theme	YM	770:771	arg1	value					773:777	the highest YM value	758:777	the highest YM value as 52.35MPa	758:789	Chitosan film with 20% CNC revealed the highest YM value as 52.35MPa.
28684354	0	6	theme	chitosan	93:100	arg1	films					102:106	chitosan films	93:106	chitosan films	93:106	Utilization of flax (Linum usitatissimum) cellulose nanocrystals as reinforcing material for chitosan films.
28684354	2	7	theme	biomaterials	269:280	arg1	use					252:254	the use	248:254	the use of different biomaterials such as chitosan and cellulose	248:311	To overcome this situation, researchers are focusing on the use of different biomaterials such as chitosan and cellulose.
28684354	5	8	attach	isolated	565:572	arg2	CNC					557:559	CNC	557:559	CNC	557:559	CNC was isolated from flax fiber using acid hydrolysis method.
28684354	5	8	attach	isolated	565:572	arg1	fiber					584:588	flax fiber	579:588	flax fiber	579:588	CNC was isolated from flax fiber using acid hydrolysis method.
28684354	5	9	theme	acid	596:599	arg1	method					612:617	acid hydrolysis method	596:617	acid hydrolysis method	596:617	CNC was isolated from flax fiber using acid hydrolysis method.
28684354	6	10	theme	concentration	707:719	arg1	increase					691:698	the increase	687:698	the increase of CNC concentration	687:719	Tensile strength (TS) and young modulus (YM) values increased with the increase of CNC concentration.
28684354	2	11	theme	different	259:267	arg1	biomaterials					269:280	different biomaterials	259:280	different biomaterials	259:280	To overcome this situation, researchers are focusing on the use of different biomaterials such as chitosan and cellulose.
28684354	9	12	theme	acceptable	956:965	arg1	level					967:971	an acceptable level	953:971	an acceptable level	953:971	All the films were observed to be transparent up to an acceptable level.
28684354	3	13	theme	barrier	443:449	arg1	properties					451:460	mechanical and barrier properties	428:460	properties	451:460	The current study was conducted to check the effect of flax (Linum usitatissimum) cellulose nanocrystals (CNC) on mechanical and barrier properties of chitosan-based films.
28684354	0	14	theme	cellulose	42:50	arg1	nanocrystals					52:63	cellulose nanocrystals	42:63	Utilization of flax (Linum usitatissimum) cellulose nanocrystals as reinforcing material for chitosan films.	0:107	Utilization of flax (Linum usitatissimum) cellulose nanocrystals as reinforcing material for chitosan films.
28684354	3	15	dep	flax	369:372	arg1	nanocrystals					406:417	cellulose nanocrystals	396:417	flax (Linum usitatissimum) cellulose nanocrystals (CNC)	369:423	The current study was conducted to check the effect of flax (Linum usitatissimum) cellulose nanocrystals (CNC) on mechanical and barrier properties of chitosan-based films.
28684354	3	15	dep	flax	369:372	arg1	CNC					420:422	CNC	420:422	CNC	420:422	The current study was conducted to check the effect of flax (Linum usitatissimum) cellulose nanocrystals (CNC) on mechanical and barrier properties of chitosan-based films.
28684354	3	15	dep	flax	369:372	arg1	usitatissimum					381:393	Linum usitatissimum	375:393	Linum usitatissimum	375:393	The current study was conducted to check the effect of flax (Linum usitatissimum) cellulose nanocrystals (CNC) on mechanical and barrier properties of chitosan-based films.
28684354	6	16	theme	CNC	703:705	arg1	concentration					707:719	CNC concentration	703:719	CNC concentration	703:719	Tensile strength (TS) and young modulus (YM) values increased with the increase of CNC concentration.
28684354	7	17	theme	highest	762:768	arg1	value					773:777	the highest YM value	758:777	the highest YM value as 52.35MPa	758:789	Chitosan film with 20% CNC revealed the highest YM value as 52.35MPa.
28684354	3	18	from	effect	359:364	arg1	properties					451:460	mechanical and barrier properties	428:460	properties	451:460	The current study was conducted to check the effect of flax (Linum usitatissimum) cellulose nanocrystals (CNC) on mechanical and barrier properties of chitosan-based films.
28684354	12	19	theme	composites	1193:1202	arg1	stability					1180:1188	the thermal stability	1168:1188	the thermal stability of composites	1168:1202	No significant improvement revealed in the thermal stability of composites.
28684354	10	20	theme	films	1024:1028	arg1	homogeneity					1009:1019	the homogeneity	1005:1019	the homogeneity of films	1005:1028	SEM and AFM analysis confirmed the homogeneity of films.
28684354	10	21	gly	homogeneity	1009:1019	arg1	films					1024:1028	films	1024:1028	films	1024:1028	SEM and AFM analysis confirmed the homogeneity of films.
28684354	11	22	theme	chitosan/CNC	1099:1110	arg1	films					1122:1126	chitosan/CNC composite films	1099:1126	chitosan/CNC composite films	1099:1126	A gradual enhancement was recorded in the antimicrobial activity of chitosan/CNC composite films.
28684354	6	23	theme	Tensile	620:626	arg1	TS					638:639	TS	638:639	TS	638:639	Tensile strength (TS) and young modulus (YM) values increased with the increase of CNC concentration.
28684354	6	23	theme	Tensile	620:626	arg1	strength					628:635	Tensile strength	620:635	Tensile strength (TS)	620:640	Tensile strength (TS) and young modulus (YM) values increased with the increase of CNC concentration.
28684354	3	24	theme	cellulose	396:404	arg1	nanocrystals					406:417	cellulose nanocrystals	396:417	flax (Linum usitatissimum) cellulose nanocrystals (CNC)	369:423	The current study was conducted to check the effect of flax (Linum usitatissimum) cellulose nanocrystals (CNC) on mechanical and barrier properties of chitosan-based films.
28684354	3	24	theme	cellulose	396:404	arg1	CNC					420:422	CNC	420:422	CNC	420:422	The current study was conducted to check the effect of flax (Linum usitatissimum) cellulose nanocrystals (CNC) on mechanical and barrier properties of chitosan-based films.
28684354	7	25	theme	Chitosan	722:729	arg1	film					731:734	Chitosan film	722:734	Chitosan film with 20% CNC	722:747	Chitosan film with 20% CNC revealed the highest YM value as 52.35MPa.
28684354	7	26	theme	20	741:742	arg1	%					743:743	%	743:743	%	743:743	Chitosan film with 20% CNC revealed the highest YM value as 52.35MPa.
28684354	11	27	theme	antimicrobial	1073:1085	arg1	activity					1087:1094	the antimicrobial activity	1069:1094	the antimicrobial activity of chitosan/CNC composite films	1069:1126	A gradual enhancement was recorded in the antimicrobial activity of chitosan/CNC composite films.
28684354	7	28	theme	%	743:743	arg1	CNC					745:747	20% CNC	741:747	20% CNC	741:747	Chitosan film with 20% CNC revealed the highest YM value as 52.35MPa.
28684354	12	29	theme	thermal	1172:1178	arg1	stability					1180:1188	the thermal stability	1168:1188	the thermal stability of composites	1168:1202	No significant improvement revealed in the thermal stability of composites.
28684354	0	30	theme	flax	15:18	arg1	Utilization					0:10	Utilization	0:10	Utilization of flax (Linum usitatissimum) cellulose nanocrystals as reinforcing material for chitosan films.	0:107	Utilization of flax (Linum usitatissimum) cellulose nanocrystals as reinforcing material for chitosan films.
28684354	7	31	with	film	731:734	arg1	CNC					745:747	20% CNC	741:747	20% CNC	741:747	Chitosan film with 20% CNC revealed the highest YM value as 52.35MPa.
28684354	8	32	theme	significant	795:805	arg1	improvement					807:817	No significant improvement	792:817	No significant improvement	792:817	No significant improvement was recorded in water vapor permeability due to overall lower film crystallinity.
28684354	3	33	theme	mechanical	428:437	arg1	properties					451:460	mechanical and barrier properties	428:460	properties	451:460	The current study was conducted to check the effect of flax (Linum usitatissimum) cellulose nanocrystals (CNC) on mechanical and barrier properties of chitosan-based films.
28684354	11	34	theme	composite	1112:1120	arg1	films					1122:1126	chitosan/CNC composite films	1099:1126	chitosan/CNC composite films	1099:1126	A gradual enhancement was recorded in the antimicrobial activity of chitosan/CNC composite films.
28684354	8	35	theme	vapor	841:845	arg1	permeability					847:858	water vapor permeability	835:858	water vapor permeability due to overall lower film crystallinity	835:898	No significant improvement was recorded in water vapor permeability due to overall lower film crystallinity.
28684354	11	36	theme	films	1122:1126	arg1	activity					1087:1094	the antimicrobial activity	1069:1094	the antimicrobial activity of chitosan/CNC composite films	1069:1126	A gradual enhancement was recorded in the antimicrobial activity of chitosan/CNC composite films.
28684354	3	37	theme	flax	369:372	arg1	effect					359:364	the effect	355:364	the effect of flax (Linum usitatissimum) cellulose nanocrystals (CNC) on mechanical and barrier properties of chitosan-based films	355:484	The current study was conducted to check the effect of flax (Linum usitatissimum) cellulose nanocrystals (CNC) on mechanical and barrier properties of chitosan-based films.
28684354	5	38	theme	hydrolysis	601:610	arg1	method					612:617	acid hydrolysis method	596:617	acid hydrolysis method	596:617	CNC was isolated from flax fiber using acid hydrolysis method.
28684354	1	39	dep	both	157:160	arg1	health					162:167	health	162:167	health	162:167	Use of plastic based packaging tools is causing both health and economic problems.
28684354	10	40	theme	AFM	982:984	arg1	analysis					986:993	AFM analysis	982:993	AFM analysis	982:993	SEM and AFM analysis confirmed the homogeneity of films.
28684354	4	41	theme	different	511:519	arg1	%					553:553	5, 10, 20 and 30%	537:553	5, 10, 20 and 30%	537:553	CNC was incorporated in different concentrations (5, 10, 20 and 30%).
28684354	4	41	theme	different	511:519	arg1	concentrations					521:534	different concentrations	511:534	different concentrations (5, 10, 20 and 30%)	511:554	CNC was incorporated in different concentrations (5, 10, 20 and 30%).
28684354	8	42	theme	water	835:839	arg1	permeability					847:858	water vapor permeability	835:858	water vapor permeability due to overall lower film crystallinity	835:898	No significant improvement was recorded in water vapor permeability due to overall lower film crystallinity.
28684354	12	43	theme	significant	1132:1142	arg1	improvement					1144:1154	No significant improvement	1129:1154	No significant improvement	1129:1154	No significant improvement revealed in the thermal stability of composites.
28684354	11	44	theme	gradual	1033:1039	arg1	enhancement					1041:1051	A gradual enhancement	1031:1051	A gradual enhancement	1031:1051	A gradual enhancement was recorded in the antimicrobial activity of chitosan/CNC composite films.
28684354	6	45	theme	YM	661:662	arg1	values					665:670	young modulus (YM) values	646:670	young modulus (YM) values	646:670	Tensile strength (TS) and young modulus (YM) values increased with the increase of CNC concentration.
28684354	1	46	theme	plastic	116:122	arg1	tools					140:144	plastic based packaging tools	116:144	plastic based packaging tools	116:144	Use of plastic based packaging tools is causing both health and economic problems.
28684354	9	47	dep	level	967:971	arg1	up					947:948	up	947:948	up	947:948	All the films were observed to be transparent up to an acceptable level.
28684354	1	48	theme	based	124:128	arg1	tools					140:144	plastic based packaging tools	116:144	plastic based packaging tools	116:144	Use of plastic based packaging tools is causing both health and economic problems.
28684354	1	49	dep	problems	182:189	arg1	both					157:160	both	157:160	both	157:160	Use of plastic based packaging tools is causing both health and economic problems.
28684354	1	49	dep	problems	182:189	arg1	economic					173:180	economic	173:180	economic	173:180	Use of plastic based packaging tools is causing both health and economic problems.
28684354	6	50	theme	modulus	652:658	arg1	values					665:670	young modulus (YM) values	646:670	young modulus (YM) values	646:670	Tensile strength (TS) and young modulus (YM) values increased with the increase of CNC concentration.
28684354	1	51	theme	packaging	130:138	arg1	tools					140:144	plastic based packaging tools	116:144	plastic based packaging tools	116:144	Use of plastic based packaging tools is causing both health and economic problems.
28684354	8	52	theme	film	881:884	arg1	crystallinity					886:898	overall lower film crystallinity	867:898	overall lower film crystallinity	867:898	No significant improvement was recorded in water vapor permeability due to overall lower film crystallinity.
28684354	3	53	theme	current	318:324	arg1	study					326:330	The current study	314:330	The current study	314:330	The current study was conducted to check the effect of flax (Linum usitatissimum) cellulose nanocrystals (CNC) on mechanical and barrier properties of chitosan-based films.
28684354	0	54	dep	Utilization	0:10	arg1	usitatissimum					27:39	Linum usitatissimum	21:39	Utilization of flax (Linum usitatissimum) cellulose nanocrystals as reinforcing material for chitosan films.	0:107	Utilization of flax (Linum usitatissimum) cellulose nanocrystals as reinforcing material for chitosan films.
28684354	0	54	dep	Utilization	0:10	arg1	nanocrystals					52:63	cellulose nanocrystals	42:63	Utilization of flax (Linum usitatissimum) cellulose nanocrystals as reinforcing material for chitosan films.	0:107	Utilization of flax (Linum usitatissimum) cellulose nanocrystals as reinforcing material for chitosan films.
28684354	6	55	theme	young	646:650	arg1	values					665:670	young modulus (YM) values	646:670	young modulus (YM) values	646:670	Tensile strength (TS) and young modulus (YM) values increased with the increase of CNC concentration.
28684354	1	56	theme	tools	140:144	arg1	Use					109:111	Use	109:111	Use of plastic based packaging tools	109:144	Use of plastic based packaging tools is causing both health and economic problems.
28530283	7	0	theme	IBU-CD-MOF/PAA	1648:1661	arg1	microspheres					1673:1684	IBU-CD-MOF/PAA composite microspheres	1648:1684	IBU-CD-MOF/PAA composite microspheres	1648:1684	The cumulative release rate for IBU (and LPZ) follows the trend: IBU-γ-CD complex microspheres (ca. 80% in 2 h) > IBU microspheres > IBU-CD-MOF/PAA composite microspheres (ca. 50% in 24 h).
28530283	3	1	theme	polyacrylic	461:471	arg1	PAA					479:481	PAA	479:481	PAA	479:481	In the investigation reported herein, composites of γ-cyclodextrin (γ-CD)-based MOFs (CD-MOFs) and polyacrylic acid (PAA) were prepared by a solid in oil-in-oil (s/o/o) emulsifying solvent evaporation method.
28530283	3	1	theme	polyacrylic	461:471	arg1	acid					473:476	polyacrylic acid	461:476	polyacrylic acid (PAA)	461:482	In the investigation reported herein, composites of γ-cyclodextrin (γ-CD)-based MOFs (CD-MOFs) and polyacrylic acid (PAA) were prepared by a solid in oil-in-oil (s/o/o) emulsifying solvent evaporation method.
28530283	4	2	theme	X-ray	786:790	arg1	diffraction					792:802	powder X-ray diffraction	779:802	powder X-ray diffraction	779:802	A modified hydrothermal protocol has been established which produces efficiently at 50 °C in 6 h micron (5-10 μm) and nanometer (500-700 nm) diameter CD-MOF particles of uniform size with smooth surfaces and powder X-ray diffraction patterns that are identical with those reported in the literature.
28530283	9	3	from	embedded	1990:1997	arg1	matrix					2032:2037	a biocompatible polymer (PAA) matrix	2002:2037	a biocompatible polymer (PAA) matrix	2002:2037	In summary, these composite microspheres, composed of CD-MOF nanocrystals embedded in a biocompatible polymer (PAA) matrix, constitute an efficient and pharmaceutically acceptable MOF-based carrier for sustained drug release.
28530283	6	4	theme	prolonged	1440:1448	arg1	period					1450:1455	a prolonged period	1438:1455	a prolonged period of time	1438:1463	On account of the good dispersion of drug-loaded CD-MOF nanocrystals inside polyacrylic acid (PAA) matrices and the homogeneous distribution of the drug molecules within these crystals, the composite microspheres exhibit not only spherical shapes and sustained drug release over a prolonged period of time, but they also demonstrate reduced cell toxicity.
28530283	4	5	theme	size	749:752	arg1	particles					728:736	CD-MOF particles	721:736	6 h micron (5-10 μm) and nanometer (500-700 nm) diameter CD-MOF particles of uniform size with smooth surfaces	664:773	A modified hydrothermal protocol has been established which produces efficiently at 50 °C in 6 h micron (5-10 μm) and nanometer (500-700 nm) diameter CD-MOF particles of uniform size with smooth surfaces and powder X-ray diffraction patterns that are identical with those reported in the literature.
28530283	5	6	dep	both	911:914	arg1	insoluble					916:924	insoluble	916:924	insoluble	916:924	Ibuprofen (IBU) and Lansoprazole (LPZ), both insoluble in water and lacking in stability, were entrapped with high drug loading in nanometer-sized CD-MOFs by co-crystallisation (that is more effective than impregnation) without causing MOF crystal degradation during the loading process.
28530283	9	7	theme	composite	1934:1942	arg1	microspheres					1944:1955	these composite microspheres	1928:1955	these composite microspheres	1928:1955	In summary, these composite microspheres, composed of CD-MOF nanocrystals embedded in a biocompatible polymer (PAA) matrix, constitute an efficient and pharmaceutically acceptable MOF-based carrier for sustained drug release.
28530283	4	8	theme	smooth	759:764	arg1	surfaces					766:773	smooth surfaces	759:773	smooth surfaces	759:773	A modified hydrothermal protocol has been established which produces efficiently at 50 °C in 6 h micron (5-10 μm) and nanometer (500-700 nm) diameter CD-MOF particles of uniform size with smooth surfaces and powder X-ray diffraction patterns that are identical with those reported in the literature.
28530283	7	9	dep	%	1693:1693	arg1	ca.					1687:1689	ca.	1687:1689	ca.	1687:1689	The cumulative release rate for IBU (and LPZ) follows the trend: IBU-γ-CD complex microspheres (ca. 80% in 2 h) > IBU microspheres > IBU-CD-MOF/PAA composite microspheres (ca. 50% in 24 h).
28530283	3	10	theme	in	509:510	arg1	method					563:568	a solid in oil-in-oil (s/o/o) emulsifying solvent evaporation method	501:568	a solid in oil-in-oil (s/o/o) emulsifying solvent evaporation method	501:568	In the investigation reported herein, composites of γ-cyclodextrin (γ-CD)-based MOFs (CD-MOFs) and polyacrylic acid (PAA) were prepared by a solid in oil-in-oil (s/o/o) emulsifying solvent evaporation method.
28530283	6	11	theme	drug	1420:1423	arg1	release					1425:1431	drug release	1420:1431	drug release	1420:1431	On account of the good dispersion of drug-loaded CD-MOF nanocrystals inside polyacrylic acid (PAA) matrices and the homogeneous distribution of the drug molecules within these crystals, the composite microspheres exhibit not only spherical shapes and sustained drug release over a prolonged period of time, but they also demonstrate reduced cell toxicity.
28530283	8	12	theme	burst	1721:1725	arg1	release					1727:1733	no burst release	1718:1733	no burst release of IBU (and LPZ)	1718:1750	Importantly, no burst release of IBU (and LPZ) was observed from the CD-MOF/PAA composite microspheres, suggesting an even distribution of the drug as well as strong drug carrier interactions inside the CD-MOF.
28530283	1	13	theme	new	198:200	arg1	class					202:206	a new class	196:206	a new class of carriers for sustained drug delivery	196:246	Metal-organic frameworks (MOFs), which are typically embedded in polymer matrices as composites, are emerging as a new class of carriers for sustained drug delivery.
28530283	7	14	theme	cumulative	1519:1528	arg1	rate					1538:1541	The cumulative release rate	1515:1541	The cumulative release rate for IBU (and LPZ)	1515:1559	The cumulative release rate for IBU (and LPZ) follows the trend: IBU-γ-CD complex microspheres (ca. 80% in 2 h) > IBU microspheres > IBU-CD-MOF/PAA composite microspheres (ca. 50% in 24 h).
28530283	4	15	theme	hydrothermal	582:593	arg1	protocol					595:602	A modified hydrothermal protocol	571:602	A modified hydrothermal protocol	571:602	A modified hydrothermal protocol has been established which produces efficiently at 50 °C in 6 h micron (5-10 μm) and nanometer (500-700 nm) diameter CD-MOF particles of uniform size with smooth surfaces and powder X-ray diffraction patterns that are identical with those reported in the literature.
28530283	6	16	theme	CD-MOF	1208:1213	arg1	nanocrystals					1215:1226	drug-loaded CD-MOF nanocrystals	1196:1226	drug-loaded CD-MOF nanocrystals inside polyacrylic acid (PAA) matrices	1196:1265	On account of the good dispersion of drug-loaded CD-MOF nanocrystals inside polyacrylic acid (PAA) matrices and the homogeneous distribution of the drug molecules within these crystals, the composite microspheres exhibit not only spherical shapes and sustained drug release over a prolonged period of time, but they also demonstrate reduced cell toxicity.
28530283	4	17	theme	6	664:664	arg1	h					666:666	h	666:666	h	666:666	A modified hydrothermal protocol has been established which produces efficiently at 50 °C in 6 h micron (5-10 μm) and nanometer (500-700 nm) diameter CD-MOF particles of uniform size with smooth surfaces and powder X-ray diffraction patterns that are identical with those reported in the literature.
28530283	9	18	theme	biocompatible	2004:2016	arg1	PAA					2027:2029	PAA	2027:2029	PAA	2027:2029	In summary, these composite microspheres, composed of CD-MOF nanocrystals embedded in a biocompatible polymer (PAA) matrix, constitute an efficient and pharmaceutically acceptable MOF-based carrier for sustained drug release.
28530283	9	18	theme	biocompatible	2004:2016	arg1	polymer					2018:2024	a biocompatible polymer	2002:2024	a biocompatible polymer (PAA) matrix	2002:2037	In summary, these composite microspheres, composed of CD-MOF nanocrystals embedded in a biocompatible polymer (PAA) matrix, constitute an efficient and pharmaceutically acceptable MOF-based carrier for sustained drug release.
28530283	6	19	theme	good	1177:1180	arg1	dispersion					1182:1191	the good dispersion	1173:1191	the good dispersion of drug-loaded CD-MOF nanocrystals inside polyacrylic acid (PAA) matrices	1173:1265	On account of the good dispersion of drug-loaded CD-MOF nanocrystals inside polyacrylic acid (PAA) matrices and the homogeneous distribution of the drug molecules within these crystals, the composite microspheres exhibit not only spherical shapes and sustained drug release over a prolonged period of time, but they also demonstrate reduced cell toxicity.
28530283	0	20	theme	nanocrystals-containing	17:39	arg1	microspheres					41:52	Composite CD-MOF nanocrystals-containing microspheres	0:52	Composite CD-MOF nanocrystals-containing microspheres for sustained drug delivery	0:80	Composite CD-MOF nanocrystals-containing microspheres for sustained drug delivery.
28530283	7	21	theme	IBU-γ-CD	1580:1587	arg1	microspheres					1597:1608	IBU-γ-CD complex microspheres	1580:1608	IBU-γ-CD complex microspheres (ca. 80% in 2 h) > IBU microspheres	1580:1644	The cumulative release rate for IBU (and LPZ) follows the trend: IBU-γ-CD complex microspheres (ca. 80% in 2 h) > IBU microspheres > IBU-CD-MOF/PAA composite microspheres (ca. 50% in 24 h).
28530283	8	22	theme	LPZ	1747:1749	arg1	release					1727:1733	no burst release	1718:1733	no burst release of IBU (and LPZ)	1718:1750	Importantly, no burst release of IBU (and LPZ) was observed from the CD-MOF/PAA composite microspheres, suggesting an even distribution of the drug as well as strong drug carrier interactions inside the CD-MOF.
28530283	5	23	theme	loading	1142:1148	arg1	process					1150:1156	the loading process	1138:1156	the loading process	1138:1156	Ibuprofen (IBU) and Lansoprazole (LPZ), both insoluble in water and lacking in stability, were entrapped with high drug loading in nanometer-sized CD-MOFs by co-crystallisation (that is more effective than impregnation) without causing MOF crystal degradation during the loading process.
28530283	5	24	from	water	929:933	arg1	insoluble					916:924	insoluble	916:924	insoluble	916:924	Ibuprofen (IBU) and Lansoprazole (LPZ), both insoluble in water and lacking in stability, were entrapped with high drug loading in nanometer-sized CD-MOFs by co-crystallisation (that is more effective than impregnation) without causing MOF crystal degradation during the loading process.
28530283	5	25	theme	nanometer-sized	1002:1016	arg1	CD-MOFs					1018:1024	nanometer-sized CD-MOFs	1002:1024	nanometer-sized CD-MOFs	1002:1024	Ibuprofen (IBU) and Lansoprazole (LPZ), both insoluble in water and lacking in stability, were entrapped with high drug loading in nanometer-sized CD-MOFs by co-crystallisation (that is more effective than impregnation) without causing MOF crystal degradation during the loading process.
28530283	6	26	theme	PAA	1253:1255	arg1	matrices					1258:1265	polyacrylic acid (PAA) matrices	1235:1265	polyacrylic acid (PAA) matrices	1235:1265	On account of the good dispersion of drug-loaded CD-MOF nanocrystals inside polyacrylic acid (PAA) matrices and the homogeneous distribution of the drug molecules within these crystals, the composite microspheres exhibit not only spherical shapes and sustained drug release over a prolonged period of time, but they also demonstrate reduced cell toxicity.
28530283	1	27	theme	Metal-organic	83:95	arg1	frameworks					97:106	Metal-organic frameworks	83:106	Metal-organic frameworks (MOFs)	83:113	Metal-organic frameworks (MOFs), which are typically embedded in polymer matrices as composites, are emerging as a new class of carriers for sustained drug delivery.
28530283	1	27	theme	Metal-organic	83:95	arg1	MOFs					109:112	MOFs	109:112	MOFs	109:112	Metal-organic frameworks (MOFs), which are typically embedded in polymer matrices as composites, are emerging as a new class of carriers for sustained drug delivery.
28530283	9	28	theme	MOF-based	2096:2104	arg1	carrier					2106:2112	an efficient and pharmaceutically acceptable MOF-based carrier	2051:2112	an efficient and pharmaceutically acceptable MOF-based carrier for sustained drug release	2051:2139	In summary, these composite microspheres, composed of CD-MOF nanocrystals embedded in a biocompatible polymer (PAA) matrix, constitute an efficient and pharmaceutically acceptable MOF-based carrier for sustained drug release.
28530283	4	29	theme	nanometer	689:697	arg1	nm					708:709	500-700 nm	700:709	500-700 nm	700:709	A modified hydrothermal protocol has been established which produces efficiently at 50 °C in 6 h micron (5-10 μm) and nanometer (500-700 nm) diameter CD-MOF particles of uniform size with smooth surfaces and powder X-ray diffraction patterns that are identical with those reported in the literature.
28530283	4	29	theme	nanometer	689:697	arg1	diameter					712:719	6 h micron (5-10 μm) and nanometer (500-700 nm) diameter	664:719	diameter	712:719	A modified hydrothermal protocol has been established which produces efficiently at 50 °C in 6 h micron (5-10 μm) and nanometer (500-700 nm) diameter CD-MOF particles of uniform size with smooth surfaces and powder X-ray diffraction patterns that are identical with those reported in the literature.
28530283	6	30	theme	acid	1247:1250	arg1	matrices					1258:1265	polyacrylic acid (PAA) matrices	1235:1265	polyacrylic acid (PAA) matrices	1235:1265	On account of the good dispersion of drug-loaded CD-MOF nanocrystals inside polyacrylic acid (PAA) matrices and the homogeneous distribution of the drug molecules within these crystals, the composite microspheres exhibit not only spherical shapes and sustained drug release over a prolonged period of time, but they also demonstrate reduced cell toxicity.
28530283	5	31	theme	high	981:984	arg1	loading					991:997	high drug loading	981:997	high drug loading in nanometer-sized CD-MOFs by co-crystallisation (that is more effective than impregnation)	981:1089	Ibuprofen (IBU) and Lansoprazole (LPZ), both insoluble in water and lacking in stability, were entrapped with high drug loading in nanometer-sized CD-MOFs by co-crystallisation (that is more effective than impregnation) without causing MOF crystal degradation during the loading process.
28530283	0	32	theme	sustained	58:66	arg1	delivery					73:80	sustained drug delivery	58:80	sustained drug delivery	58:80	Composite CD-MOF nanocrystals-containing microspheres for sustained drug delivery.
28530283	1	33	from	embedded	136:143	arg1	matrices					156:163	polymer matrices	148:163	polymer matrices as composites	148:177	Metal-organic frameworks (MOFs), which are typically embedded in polymer matrices as composites, are emerging as a new class of carriers for sustained drug delivery.
28530283	9	34	theme	polymer	2018:2024	arg1	matrix					2032:2037	a biocompatible polymer (PAA) matrix	2002:2037	a biocompatible polymer (PAA) matrix	2002:2037	In summary, these composite microspheres, composed of CD-MOF nanocrystals embedded in a biocompatible polymer (PAA) matrix, constitute an efficient and pharmaceutically acceptable MOF-based carrier for sustained drug release.
28530283	4	35	theme	micron	668:673	arg1	patterns					804:811	6 h micron (5-10 μm) and nanometer (500-700 nm) diameter CD-MOF particles of uniform size with smooth surfaces and powder X-ray diffraction patterns	664:811	6 h micron (5-10 μm) and nanometer (500-700 nm) diameter CD-MOF particles of uniform size with smooth surfaces and powder X-ray diffraction patterns that are identical with those reported in the literature	664:868	A modified hydrothermal protocol has been established which produces efficiently at 50 °C in 6 h micron (5-10 μm) and nanometer (500-700 nm) diameter CD-MOF particles of uniform size with smooth surfaces and powder X-ray diffraction patterns that are identical with those reported in the literature.
28530283	0	36	theme	Composite	0:8	arg1	microspheres					41:52	Composite CD-MOF nanocrystals-containing microspheres	0:52	Composite CD-MOF nanocrystals-containing microspheres for sustained drug delivery	0:80	Composite CD-MOF nanocrystals-containing microspheres for sustained drug delivery.
28530283	6	37	theme	cell	1500:1503	arg1	toxicity					1505:1512	reduced cell toxicity	1492:1512	reduced cell toxicity	1492:1512	On account of the good dispersion of drug-loaded CD-MOF nanocrystals inside polyacrylic acid (PAA) matrices and the homogeneous distribution of the drug molecules within these crystals, the composite microspheres exhibit not only spherical shapes and sustained drug release over a prolonged period of time, but they also demonstrate reduced cell toxicity.
28530283	8	38	theme	strong	1864:1869	arg1	interactions					1884:1895	strong drug carrier interactions	1864:1895	an even distribution of the drug as well as strong drug carrier interactions	1820:1895	Importantly, no burst release of IBU (and LPZ) was observed from the CD-MOF/PAA composite microspheres, suggesting an even distribution of the drug as well as strong drug carrier interactions inside the CD-MOF.
28530283	9	39	theme	acceptable	2085:2094	arg1	carrier					2106:2112	an efficient and pharmaceutically acceptable MOF-based carrier	2051:2112	an efficient and pharmaceutically acceptable MOF-based carrier for sustained drug release	2051:2139	In summary, these composite microspheres, composed of CD-MOF nanocrystals embedded in a biocompatible polymer (PAA) matrix, constitute an efficient and pharmaceutically acceptable MOF-based carrier for sustained drug release.
28530283	6	40	theme	molecules	1312:1320	arg1	distribution					1287:1298	the homogeneous distribution	1271:1298	the homogeneous distribution of the drug molecules within these crystals	1271:1342	On account of the good dispersion of drug-loaded CD-MOF nanocrystals inside polyacrylic acid (PAA) matrices and the homogeneous distribution of the drug molecules within these crystals, the composite microspheres exhibit not only spherical shapes and sustained drug release over a prolonged period of time, but they also demonstrate reduced cell toxicity.
28530283	6	40	theme	molecules	1312:1320	arg1	dispersion					1182:1191	the good dispersion	1173:1191	the good dispersion of drug-loaded CD-MOF nanocrystals inside polyacrylic acid (PAA) matrices	1173:1265	On account of the good dispersion of drug-loaded CD-MOF nanocrystals inside polyacrylic acid (PAA) matrices and the homogeneous distribution of the drug molecules within these crystals, the composite microspheres exhibit not only spherical shapes and sustained drug release over a prolonged period of time, but they also demonstrate reduced cell toxicity.
28530283	8	41	theme	carrier	1876:1882	arg1	interactions					1884:1895	strong drug carrier interactions	1864:1895	an even distribution of the drug as well as strong drug carrier interactions	1820:1895	Importantly, no burst release of IBU (and LPZ) was observed from the CD-MOF/PAA composite microspheres, suggesting an even distribution of the drug as well as strong drug carrier interactions inside the CD-MOF.
28530283	4	42	theme	CD-MOF	721:726	arg1	particles					728:736	CD-MOF particles	721:736	6 h micron (5-10 μm) and nanometer (500-700 nm) diameter CD-MOF particles of uniform size with smooth surfaces	664:773	A modified hydrothermal protocol has been established which produces efficiently at 50 °C in 6 h micron (5-10 μm) and nanometer (500-700 nm) diameter CD-MOF particles of uniform size with smooth surfaces and powder X-ray diffraction patterns that are identical with those reported in the literature.
28530283	7	43	from	%	1693:1693	arg1	h					1701:1701	24 h	1698:1701	24 h	1698:1701	The cumulative release rate for IBU (and LPZ) follows the trend: IBU-γ-CD complex microspheres (ca. 80% in 2 h) > IBU microspheres > IBU-CD-MOF/PAA composite microspheres (ca. 50% in 24 h).
28530283	9	44	theme	sustained	2118:2126	arg1	release					2133:2139	sustained drug release	2118:2139	sustained drug release	2118:2139	In summary, these composite microspheres, composed of CD-MOF nanocrystals embedded in a biocompatible polymer (PAA) matrix, constitute an efficient and pharmaceutically acceptable MOF-based carrier for sustained drug release.
28530283	0	45	theme	drug	68:71	arg1	delivery					73:80	sustained drug delivery	58:80	sustained drug delivery	58:80	Composite CD-MOF nanocrystals-containing microspheres for sustained drug delivery.
28530283	3	46	theme	solvent	543:549	arg1	method					563:568	a solid in oil-in-oil (s/o/o) emulsifying solvent evaporation method	501:568	a solid in oil-in-oil (s/o/o) emulsifying solvent evaporation method	501:568	In the investigation reported herein, composites of γ-cyclodextrin (γ-CD)-based MOFs (CD-MOFs) and polyacrylic acid (PAA) were prepared by a solid in oil-in-oil (s/o/o) emulsifying solvent evaporation method.
28530283	3	47	theme	MOFs	442:445	arg1	MOFs					442:445	γ-cyclodextrin (γ-CD)-based MOFs	414:445	γ-cyclodextrin (γ-CD)-based MOFs (CD-MOFs)	414:455	In the investigation reported herein, composites of γ-cyclodextrin (γ-CD)-based MOFs (CD-MOFs) and polyacrylic acid (PAA) were prepared by a solid in oil-in-oil (s/o/o) emulsifying solvent evaporation method.
28530283	3	47	theme	MOFs	442:445	arg1	acid					473:476	polyacrylic acid	461:476	polyacrylic acid (PAA)	461:482	In the investigation reported herein, composites of γ-cyclodextrin (γ-CD)-based MOFs (CD-MOFs) and polyacrylic acid (PAA) were prepared by a solid in oil-in-oil (s/o/o) emulsifying solvent evaporation method.
28530283	3	47	theme	MOFs	442:445	arg1	composites					400:409	composites	400:409	composites of γ-cyclodextrin (γ-CD)-based MOFs (CD-MOFs) and polyacrylic acid (PAA)	400:482	In the investigation reported herein, composites of γ-cyclodextrin (γ-CD)-based MOFs (CD-MOFs) and polyacrylic acid (PAA) were prepared by a solid in oil-in-oil (s/o/o) emulsifying solvent evaporation method.
28530283	1	48	theme	carriers	211:218	arg1	class					202:206	a new class	196:206	a new class of carriers for sustained drug delivery	196:246	Metal-organic frameworks (MOFs), which are typically embedded in polymer matrices as composites, are emerging as a new class of carriers for sustained drug delivery.
28530283	7	49	theme	composite	1663:1671	arg1	microspheres					1673:1684	IBU-CD-MOF/PAA composite microspheres	1648:1684	IBU-CD-MOF/PAA composite microspheres	1648:1684	The cumulative release rate for IBU (and LPZ) follows the trend: IBU-γ-CD complex microspheres (ca. 80% in 2 h) > IBU microspheres > IBU-CD-MOF/PAA composite microspheres (ca. 50% in 24 h).
28530283	3	50	theme	acid	473:476	arg1	MOFs					442:445	γ-cyclodextrin (γ-CD)-based MOFs	414:445	γ-cyclodextrin (γ-CD)-based MOFs (CD-MOFs)	414:455	In the investigation reported herein, composites of γ-cyclodextrin (γ-CD)-based MOFs (CD-MOFs) and polyacrylic acid (PAA) were prepared by a solid in oil-in-oil (s/o/o) emulsifying solvent evaporation method.
28530283	3	50	theme	acid	473:476	arg1	acid					473:476	polyacrylic acid	461:476	polyacrylic acid (PAA)	461:482	In the investigation reported herein, composites of γ-cyclodextrin (γ-CD)-based MOFs (CD-MOFs) and polyacrylic acid (PAA) were prepared by a solid in oil-in-oil (s/o/o) emulsifying solvent evaporation method.
28530283	3	50	theme	acid	473:476	arg1	composites					400:409	composites	400:409	composites of γ-cyclodextrin (γ-CD)-based MOFs (CD-MOFs) and polyacrylic acid (PAA)	400:482	In the investigation reported herein, composites of γ-cyclodextrin (γ-CD)-based MOFs (CD-MOFs) and polyacrylic acid (PAA) were prepared by a solid in oil-in-oil (s/o/o) emulsifying solvent evaporation method.
28530283	1	51	theme	sustained	224:232	arg1	delivery					239:246	sustained drug delivery	224:246	sustained drug delivery	224:246	Metal-organic frameworks (MOFs), which are typically embedded in polymer matrices as composites, are emerging as a new class of carriers for sustained drug delivery.
28530283	7	52	dep	%	1617:1617	arg1	ca.					1611:1613	ca.	1611:1613	ca.	1611:1613	The cumulative release rate for IBU (and LPZ) follows the trend: IBU-γ-CD complex microspheres (ca. 80% in 2 h) > IBU microspheres > IBU-CD-MOF/PAA composite microspheres (ca. 50% in 24 h).
28530283	6	53	theme	composite	1349:1357	arg1	microspheres					1359:1370	the composite microspheres	1345:1370	the composite microspheres	1345:1370	On account of the good dispersion of drug-loaded CD-MOF nanocrystals inside polyacrylic acid (PAA) matrices and the homogeneous distribution of the drug molecules within these crystals, the composite microspheres exhibit not only spherical shapes and sustained drug release over a prolonged period of time, but they also demonstrate reduced cell toxicity.
28530283	7	54	theme	IBU	1629:1631	arg1	microspheres					1633:1644	IBU microspheres	1629:1644	IBU microspheres	1629:1644	The cumulative release rate for IBU (and LPZ) follows the trend: IBU-γ-CD complex microspheres (ca. 80% in 2 h) > IBU microspheres > IBU-CD-MOF/PAA composite microspheres (ca. 50% in 24 h).
28530283	4	55	theme	powder	779:784	arg1	diffraction					792:802	powder X-ray diffraction	779:802	powder X-ray diffraction	779:802	A modified hydrothermal protocol has been established which produces efficiently at 50 °C in 6 h micron (5-10 μm) and nanometer (500-700 nm) diameter CD-MOF particles of uniform size with smooth surfaces and powder X-ray diffraction patterns that are identical with those reported in the literature.
28530283	8	56	theme	drug	1848:1851	arg1	interactions					1884:1895	strong drug carrier interactions	1864:1895	an even distribution of the drug as well as strong drug carrier interactions	1820:1895	Importantly, no burst release of IBU (and LPZ) was observed from the CD-MOF/PAA composite microspheres, suggesting an even distribution of the drug as well as strong drug carrier interactions inside the CD-MOF.
28530283	8	56	theme	drug	1848:1851	arg1	distribution					1828:1839	an even distribution	1820:1839	an even distribution of the drug as well as strong drug carrier interactions	1820:1895	Importantly, no burst release of IBU (and LPZ) was observed from the CD-MOF/PAA composite microspheres, suggesting an even distribution of the drug as well as strong drug carrier interactions inside the CD-MOF.
28530283	4	57	theme	uniform	741:747	arg1	size					749:752	uniform size	741:752	uniform size	741:752	A modified hydrothermal protocol has been established which produces efficiently at 50 °C in 6 h micron (5-10 μm) and nanometer (500-700 nm) diameter CD-MOF particles of uniform size with smooth surfaces and powder X-ray diffraction patterns that are identical with those reported in the literature.
28530283	3	58	theme	solid	503:507	arg1	method					563:568	a solid in oil-in-oil (s/o/o) emulsifying solvent evaporation method	501:568	a solid in oil-in-oil (s/o/o) emulsifying solvent evaporation method	501:568	In the investigation reported herein, composites of γ-cyclodextrin (γ-CD)-based MOFs (CD-MOFs) and polyacrylic acid (PAA) were prepared by a solid in oil-in-oil (s/o/o) emulsifying solvent evaporation method.
28530283	7	59	theme	release	1530:1536	arg1	rate					1538:1541	The cumulative release rate	1515:1541	The cumulative release rate for IBU (and LPZ)	1515:1559	The cumulative release rate for IBU (and LPZ) follows the trend: IBU-γ-CD complex microspheres (ca. 80% in 2 h) > IBU microspheres > IBU-CD-MOF/PAA composite microspheres (ca. 50% in 24 h).
28530283	7	60	dep	>	1646:1646	arg1	%					1693:1693	50%	1691:1693	50%	1691:1693	The cumulative release rate for IBU (and LPZ) follows the trend: IBU-γ-CD complex microspheres (ca. 80% in 2 h) > IBU microspheres > IBU-CD-MOF/PAA composite microspheres (ca. 50% in 24 h).
28530283	6	61	theme	polyacrylic	1235:1245	arg1	matrices					1258:1265	polyacrylic acid (PAA) matrices	1235:1265	polyacrylic acid (PAA) matrices	1235:1265	On account of the good dispersion of drug-loaded CD-MOF nanocrystals inside polyacrylic acid (PAA) matrices and the homogeneous distribution of the drug molecules within these crystals, the composite microspheres exhibit not only spherical shapes and sustained drug release over a prolonged period of time, but they also demonstrate reduced cell toxicity.
28530283	5	62	from	insoluble	916:924	arg1	water					929:933	water	929:933	water	929:933	Ibuprofen (IBU) and Lansoprazole (LPZ), both insoluble in water and lacking in stability, were entrapped with high drug loading in nanometer-sized CD-MOFs by co-crystallisation (that is more effective than impregnation) without causing MOF crystal degradation during the loading process.
28530283	9	63	theme	CD-MOF	1970:1975	arg1	nanocrystals					1977:1988	CD-MOF nanocrystals	1970:1988	CD-MOF nanocrystals embedded in a biocompatible polymer (PAA) matrix	1970:2037	In summary, these composite microspheres, composed of CD-MOF nanocrystals embedded in a biocompatible polymer (PAA) matrix, constitute an efficient and pharmaceutically acceptable MOF-based carrier for sustained drug release.
28530283	6	64	theme	nanocrystals	1215:1226	arg1	distribution					1287:1298	the homogeneous distribution	1271:1298	the homogeneous distribution of the drug molecules within these crystals	1271:1342	On account of the good dispersion of drug-loaded CD-MOF nanocrystals inside polyacrylic acid (PAA) matrices and the homogeneous distribution of the drug molecules within these crystals, the composite microspheres exhibit not only spherical shapes and sustained drug release over a prolonged period of time, but they also demonstrate reduced cell toxicity.
28530283	6	64	theme	nanocrystals	1215:1226	arg1	dispersion					1182:1191	the good dispersion	1173:1191	the good dispersion of drug-loaded CD-MOF nanocrystals inside polyacrylic acid (PAA) matrices	1173:1265	On account of the good dispersion of drug-loaded CD-MOF nanocrystals inside polyacrylic acid (PAA) matrices and the homogeneous distribution of the drug molecules within these crystals, the composite microspheres exhibit not only spherical shapes and sustained drug release over a prolonged period of time, but they also demonstrate reduced cell toxicity.
28530283	3	65	theme	s/o/o	524:528	arg1	method					563:568	a solid in oil-in-oil (s/o/o) emulsifying solvent evaporation method	501:568	a solid in oil-in-oil (s/o/o) emulsifying solvent evaporation method	501:568	In the investigation reported herein, composites of γ-cyclodextrin (γ-CD)-based MOFs (CD-MOFs) and polyacrylic acid (PAA) were prepared by a solid in oil-in-oil (s/o/o) emulsifying solvent evaporation method.
28530283	4	66	theme	modified	573:580	arg1	protocol					595:602	A modified hydrothermal protocol	571:602	A modified hydrothermal protocol	571:602	A modified hydrothermal protocol has been established which produces efficiently at 50 °C in 6 h micron (5-10 μm) and nanometer (500-700 nm) diameter CD-MOF particles of uniform size with smooth surfaces and powder X-ray diffraction patterns that are identical with those reported in the literature.
28530283	9	67	theme	embedded	1990:1997	arg1	nanocrystals					1977:1988	CD-MOF nanocrystals	1970:1988	CD-MOF nanocrystals embedded in a biocompatible polymer (PAA) matrix	1970:2037	In summary, these composite microspheres, composed of CD-MOF nanocrystals embedded in a biocompatible polymer (PAA) matrix, constitute an efficient and pharmaceutically acceptable MOF-based carrier for sustained drug release.
28530283	6	68	theme	drug-loaded	1196:1206	arg1	nanocrystals					1215:1226	drug-loaded CD-MOF nanocrystals	1196:1226	drug-loaded CD-MOF nanocrystals inside polyacrylic acid (PAA) matrices	1196:1265	On account of the good dispersion of drug-loaded CD-MOF nanocrystals inside polyacrylic acid (PAA) matrices and the homogeneous distribution of the drug molecules within these crystals, the composite microspheres exhibit not only spherical shapes and sustained drug release over a prolonged period of time, but they also demonstrate reduced cell toxicity.
28530283	3	69	theme	oil-in-oil	512:521	arg1	method					563:568	a solid in oil-in-oil (s/o/o) emulsifying solvent evaporation method	501:568	a solid in oil-in-oil (s/o/o) emulsifying solvent evaporation method	501:568	In the investigation reported herein, composites of γ-cyclodextrin (γ-CD)-based MOFs (CD-MOFs) and polyacrylic acid (PAA) were prepared by a solid in oil-in-oil (s/o/o) emulsifying solvent evaporation method.
28530283	4	70	theme	diffraction	792:802	arg1	patterns					804:811	6 h micron (5-10 μm) and nanometer (500-700 nm) diameter CD-MOF particles of uniform size with smooth surfaces and powder X-ray diffraction patterns	664:811	6 h micron (5-10 μm) and nanometer (500-700 nm) diameter CD-MOF particles of uniform size with smooth surfaces and powder X-ray diffraction patterns that are identical with those reported in the literature	664:868	A modified hydrothermal protocol has been established which produces efficiently at 50 °C in 6 h micron (5-10 μm) and nanometer (500-700 nm) diameter CD-MOF particles of uniform size with smooth surfaces and powder X-ray diffraction patterns that are identical with those reported in the literature.
28530283	3	71	theme	evaporation	551:561	arg1	method					563:568	a solid in oil-in-oil (s/o/o) emulsifying solvent evaporation method	501:568	a solid in oil-in-oil (s/o/o) emulsifying solvent evaporation method	501:568	In the investigation reported herein, composites of γ-cyclodextrin (γ-CD)-based MOFs (CD-MOFs) and polyacrylic acid (PAA) were prepared by a solid in oil-in-oil (s/o/o) emulsifying solvent evaporation method.
28530283	4	72	dep	micron	668:673	arg1	particles					728:736	CD-MOF particles	721:736	6 h micron (5-10 μm) and nanometer (500-700 nm) diameter CD-MOF particles of uniform size with smooth surfaces	664:773	A modified hydrothermal protocol has been established which produces efficiently at 50 °C in 6 h micron (5-10 μm) and nanometer (500-700 nm) diameter CD-MOF particles of uniform size with smooth surfaces and powder X-ray diffraction patterns that are identical with those reported in the literature.
28530283	8	73	dep	suggesting	1809:1818	arg1	inside					1897:1902	inside	1897:1902	suggesting an even distribution of the drug as well as strong drug carrier interactions inside the CD-MOF	1809:1913	Importantly, no burst release of IBU (and LPZ) was observed from the CD-MOF/PAA composite microspheres, suggesting an even distribution of the drug as well as strong drug carrier interactions inside the CD-MOF.
28530283	4	74	with	particles	728:736	arg1	surfaces					766:773	smooth surfaces	759:773	smooth surfaces	759:773	A modified hydrothermal protocol has been established which produces efficiently at 50 °C in 6 h micron (5-10 μm) and nanometer (500-700 nm) diameter CD-MOF particles of uniform size with smooth surfaces and powder X-ray diffraction patterns that are identical with those reported in the literature.
28530283	6	75	theme	homogeneous	1275:1285	arg1	distribution					1287:1298	the homogeneous distribution	1271:1298	the homogeneous distribution of the drug molecules within these crystals	1271:1342	On account of the good dispersion of drug-loaded CD-MOF nanocrystals inside polyacrylic acid (PAA) matrices and the homogeneous distribution of the drug molecules within these crystals, the composite microspheres exhibit not only spherical shapes and sustained drug release over a prolonged period of time, but they also demonstrate reduced cell toxicity.
28530283	3	76	theme	emulsifying	531:541	arg1	method					563:568	a solid in oil-in-oil (s/o/o) emulsifying solvent evaporation method	501:568	a solid in oil-in-oil (s/o/o) emulsifying solvent evaporation method	501:568	In the investigation reported herein, composites of γ-cyclodextrin (γ-CD)-based MOFs (CD-MOFs) and polyacrylic acid (PAA) were prepared by a solid in oil-in-oil (s/o/o) emulsifying solvent evaporation method.
28530283	6	77	theme	time	1460:1463	arg1	period					1450:1455	a prolonged period	1438:1455	a prolonged period of time	1438:1463	On account of the good dispersion of drug-loaded CD-MOF nanocrystals inside polyacrylic acid (PAA) matrices and the homogeneous distribution of the drug molecules within these crystals, the composite microspheres exhibit not only spherical shapes and sustained drug release over a prolonged period of time, but they also demonstrate reduced cell toxicity.
28530283	1	78	theme	polymer	148:154	arg1	matrices					156:163	polymer matrices	148:163	polymer matrices as composites	148:177	Metal-organic frameworks (MOFs), which are typically embedded in polymer matrices as composites, are emerging as a new class of carriers for sustained drug delivery.
28530283	8	79	theme	even	1823:1826	arg1	distribution					1828:1839	an even distribution	1820:1839	an even distribution of the drug as well as strong drug carrier interactions	1820:1895	Importantly, no burst release of IBU (and LPZ) was observed from the CD-MOF/PAA composite microspheres, suggesting an even distribution of the drug as well as strong drug carrier interactions inside the CD-MOF.
28530283	5	80	theme	MOF	1107:1109	arg1	degradation					1119:1129	MOF crystal degradation	1107:1129	MOF crystal degradation	1107:1129	Ibuprofen (IBU) and Lansoprazole (LPZ), both insoluble in water and lacking in stability, were entrapped with high drug loading in nanometer-sized CD-MOFs by co-crystallisation (that is more effective than impregnation) without causing MOF crystal degradation during the loading process.
28530283	8	81	theme	CD-MOF/PAA	1774:1783	arg1	microspheres					1795:1806	the CD-MOF/PAA composite microspheres	1770:1806	the CD-MOF/PAA composite microspheres	1770:1806	Importantly, no burst release of IBU (and LPZ) was observed from the CD-MOF/PAA composite microspheres, suggesting an even distribution of the drug as well as strong drug carrier interactions inside the CD-MOF.
28530283	7	82	theme	complex	1589:1595	arg1	microspheres					1597:1608	IBU-γ-CD complex microspheres	1580:1608	IBU-γ-CD complex microspheres (ca. 80% in 2 h) > IBU microspheres	1580:1644	The cumulative release rate for IBU (and LPZ) follows the trend: IBU-γ-CD complex microspheres (ca. 80% in 2 h) > IBU microspheres > IBU-CD-MOF/PAA composite microspheres (ca. 50% in 24 h).
28530283	8	83	theme	composite	1785:1793	arg1	microspheres					1795:1806	the CD-MOF/PAA composite microspheres	1770:1806	the CD-MOF/PAA composite microspheres	1770:1806	Importantly, no burst release of IBU (and LPZ) was observed from the CD-MOF/PAA composite microspheres, suggesting an even distribution of the drug as well as strong drug carrier interactions inside the CD-MOF.
28530283	1	84	from	matrices	156:163	arg1	embedded					136:143	embedded	136:143	embedded	136:143	Metal-organic frameworks (MOFs), which are typically embedded in polymer matrices as composites, are emerging as a new class of carriers for sustained drug delivery.
28530283	7	85	dep	trend	1573:1577	arg1	>					1646:1646	>	1646:1646	> IBU-CD-MOF/PAA composite microspheres (ca. 50% in 24 h)	1646:1702	The cumulative release rate for IBU (and LPZ) follows the trend: IBU-γ-CD complex microspheres (ca. 80% in 2 h) > IBU microspheres > IBU-CD-MOF/PAA composite microspheres (ca. 50% in 24 h).
28530283	9	86	theme	efficient	2054:2062	arg1	carrier					2106:2112	an efficient and pharmaceutically acceptable MOF-based carrier	2051:2112	an efficient and pharmaceutically acceptable MOF-based carrier for sustained drug release	2051:2139	In summary, these composite microspheres, composed of CD-MOF nanocrystals embedded in a biocompatible polymer (PAA) matrix, constitute an efficient and pharmaceutically acceptable MOF-based carrier for sustained drug release.
28530283	0	87	theme	CD-MOF	10:15	arg1	microspheres					41:52	Composite CD-MOF nanocrystals-containing microspheres	0:52	Composite CD-MOF nanocrystals-containing microspheres for sustained drug delivery	0:80	Composite CD-MOF nanocrystals-containing microspheres for sustained drug delivery.
28530283	5	88	theme	drug	986:989	arg1	loading					991:997	high drug loading	981:997	high drug loading in nanometer-sized CD-MOFs by co-crystallisation (that is more effective than impregnation)	981:1089	Ibuprofen (IBU) and Lansoprazole (LPZ), both insoluble in water and lacking in stability, were entrapped with high drug loading in nanometer-sized CD-MOFs by co-crystallisation (that is more effective than impregnation) without causing MOF crystal degradation during the loading process.
28530283	4	89	with	identical	822:830	arg1	those					837:841	those	837:841	those	837:841	A modified hydrothermal protocol has been established which produces efficiently at 50 °C in 6 h micron (5-10 μm) and nanometer (500-700 nm) diameter CD-MOF particles of uniform size with smooth surfaces and powder X-ray diffraction patterns that are identical with those reported in the literature.
28530283	8	90	theme	drug	1871:1874	arg1	interactions					1884:1895	strong drug carrier interactions	1864:1895	an even distribution of the drug as well as strong drug carrier interactions	1820:1895	Importantly, no burst release of IBU (and LPZ) was observed from the CD-MOF/PAA composite microspheres, suggesting an even distribution of the drug as well as strong drug carrier interactions inside the CD-MOF.
28530283	8	91	theme	IBU	1738:1740	arg1	release					1727:1733	no burst release	1718:1733	no burst release of IBU (and LPZ)	1718:1750	Importantly, no burst release of IBU (and LPZ) was observed from the CD-MOF/PAA composite microspheres, suggesting an even distribution of the drug as well as strong drug carrier interactions inside the CD-MOF.
28530283	4	92	theme	h	666:666	arg1	micron					668:673	6 h micron (5-10 μm) and nanometer (500-700 nm) diameter	664:719	micron	668:673	A modified hydrothermal protocol has been established which produces efficiently at 50 °C in 6 h micron (5-10 μm) and nanometer (500-700 nm) diameter CD-MOF particles of uniform size with smooth surfaces and powder X-ray diffraction patterns that are identical with those reported in the literature.
28530283	4	92	theme	h	666:666	arg1	μm					681:682	5-10 μm	676:682	5-10 μm	676:682	A modified hydrothermal protocol has been established which produces efficiently at 50 °C in 6 h micron (5-10 μm) and nanometer (500-700 nm) diameter CD-MOF particles of uniform size with smooth surfaces and powder X-ray diffraction patterns that are identical with those reported in the literature.
28530283	6	93	theme	reduced	1492:1498	arg1	toxicity					1505:1512	reduced cell toxicity	1492:1512	reduced cell toxicity	1492:1512	On account of the good dispersion of drug-loaded CD-MOF nanocrystals inside polyacrylic acid (PAA) matrices and the homogeneous distribution of the drug molecules within these crystals, the composite microspheres exhibit not only spherical shapes and sustained drug release over a prolonged period of time, but they also demonstrate reduced cell toxicity.
28530283	3	94	theme	-based	435:440	arg1	CD-MOFs					448:454	CD-MOFs	448:454	CD-MOFs	448:454	In the investigation reported herein, composites of γ-cyclodextrin (γ-CD)-based MOFs (CD-MOFs) and polyacrylic acid (PAA) were prepared by a solid in oil-in-oil (s/o/o) emulsifying solvent evaporation method.
28530283	3	94	theme	-based	435:440	arg1	MOFs					442:445	γ-cyclodextrin (γ-CD)-based MOFs	414:445	γ-cyclodextrin (γ-CD)-based MOFs (CD-MOFs)	414:455	In the investigation reported herein, composites of γ-cyclodextrin (γ-CD)-based MOFs (CD-MOFs) and polyacrylic acid (PAA) were prepared by a solid in oil-in-oil (s/o/o) emulsifying solvent evaporation method.
28530283	6	95	theme	drug	1307:1310	arg1	molecules					1312:1320	the drug molecules	1303:1320	the drug molecules within these crystals	1303:1342	On account of the good dispersion of drug-loaded CD-MOF nanocrystals inside polyacrylic acid (PAA) matrices and the homogeneous distribution of the drug molecules within these crystals, the composite microspheres exhibit not only spherical shapes and sustained drug release over a prolonged period of time, but they also demonstrate reduced cell toxicity.
28530283	4	96	theme	diameter	712:719	arg1	patterns					804:811	6 h micron (5-10 μm) and nanometer (500-700 nm) diameter CD-MOF particles of uniform size with smooth surfaces and powder X-ray diffraction patterns	664:811	6 h micron (5-10 μm) and nanometer (500-700 nm) diameter CD-MOF particles of uniform size with smooth surfaces and powder X-ray diffraction patterns that are identical with those reported in the literature	664:868	A modified hydrothermal protocol has been established which produces efficiently at 50 °C in 6 h micron (5-10 μm) and nanometer (500-700 nm) diameter CD-MOF particles of uniform size with smooth surfaces and powder X-ray diffraction patterns that are identical with those reported in the literature.
28530283	5	97	from	loading	991:997	arg1	CD-MOFs					1018:1024	nanometer-sized CD-MOFs	1002:1024	nanometer-sized CD-MOFs	1002:1024	Ibuprofen (IBU) and Lansoprazole (LPZ), both insoluble in water and lacking in stability, were entrapped with high drug loading in nanometer-sized CD-MOFs by co-crystallisation (that is more effective than impregnation) without causing MOF crystal degradation during the loading process.
28530283	7	98	from	%	1617:1617	arg1	h					1624:1624	2 h	1622:1624	2 h	1622:1624	The cumulative release rate for IBU (and LPZ) follows the trend: IBU-γ-CD complex microspheres (ca. 80% in 2 h) > IBU microspheres > IBU-CD-MOF/PAA composite microspheres (ca. 50% in 24 h).
28530283	9	99	from	matrix	2032:2037	arg1	embedded					1990:1997	embedded	1990:1997	embedded	1990:1997	In summary, these composite microspheres, composed of CD-MOF nanocrystals embedded in a biocompatible polymer (PAA) matrix, constitute an efficient and pharmaceutically acceptable MOF-based carrier for sustained drug release.
28530283	9	100	theme	drug	2128:2131	arg1	release					2133:2139	sustained drug release	2118:2139	sustained drug release	2118:2139	In summary, these composite microspheres, composed of CD-MOF nanocrystals embedded in a biocompatible polymer (PAA) matrix, constitute an efficient and pharmaceutically acceptable MOF-based carrier for sustained drug release.
28530283	6	101	theme	spherical	1389:1397	arg1	shapes					1399:1404	spherical shapes	1389:1404	spherical shapes	1389:1404	On account of the good dispersion of drug-loaded CD-MOF nanocrystals inside polyacrylic acid (PAA) matrices and the homogeneous distribution of the drug molecules within these crystals, the composite microspheres exhibit not only spherical shapes and sustained drug release over a prolonged period of time, but they also demonstrate reduced cell toxicity.
28530283	4	102	from	°C	658:659	arg1	patterns					804:811	6 h micron (5-10 μm) and nanometer (500-700 nm) diameter CD-MOF particles of uniform size with smooth surfaces and powder X-ray diffraction patterns	664:811	6 h micron (5-10 μm) and nanometer (500-700 nm) diameter CD-MOF particles of uniform size with smooth surfaces and powder X-ray diffraction patterns that are identical with those reported in the literature	664:868	A modified hydrothermal protocol has been established which produces efficiently at 50 °C in 6 h micron (5-10 μm) and nanometer (500-700 nm) diameter CD-MOF particles of uniform size with smooth surfaces and powder X-ray diffraction patterns that are identical with those reported in the literature.
28530283	5	103	theme	crystal	1111:1117	arg1	degradation					1119:1129	MOF crystal degradation	1107:1129	MOF crystal degradation	1107:1129	Ibuprofen (IBU) and Lansoprazole (LPZ), both insoluble in water and lacking in stability, were entrapped with high drug loading in nanometer-sized CD-MOFs by co-crystallisation (that is more effective than impregnation) without causing MOF crystal degradation during the loading process.
28530283	5	104	dep	Lansoprazole	891:902	arg1	lacking					939:945	lacking	939:945	lacking in stability	939:958	Ibuprofen (IBU) and Lansoprazole (LPZ), both insoluble in water and lacking in stability, were entrapped with high drug loading in nanometer-sized CD-MOFs by co-crystallisation (that is more effective than impregnation) without causing MOF crystal degradation during the loading process.
28530283	5	104	dep	Lansoprazole	891:902	arg1	both					911:914	both	911:914	both	911:914	Ibuprofen (IBU) and Lansoprazole (LPZ), both insoluble in water and lacking in stability, were entrapped with high drug loading in nanometer-sized CD-MOFs by co-crystallisation (that is more effective than impregnation) without causing MOF crystal degradation during the loading process.
28530283	6	105	dep	exhibit	1372:1378	arg1	only					1384:1387	only	1384:1387	only	1384:1387	On account of the good dispersion of drug-loaded CD-MOF nanocrystals inside polyacrylic acid (PAA) matrices and the homogeneous distribution of the drug molecules within these crystals, the composite microspheres exhibit not only spherical shapes and sustained drug release over a prolonged period of time, but they also demonstrate reduced cell toxicity.
28530283	1	106	theme	drug	234:237	arg1	delivery					239:246	sustained drug delivery	224:246	sustained drug delivery	224:246	Metal-organic frameworks (MOFs), which are typically embedded in polymer matrices as composites, are emerging as a new class of carriers for sustained drug delivery.
25529653	0	0	theme	edulis	94:99	arg1	proximate					42:50	proximate	42:50	proximate	42:50	Evaluation of physicochemical properties, proximate and nutritional composition of Gracilaria edulis collected from Palk Bay.
25529653	0	0	theme	edulis	94:99	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of physicochemical properties	0:39	Evaluation of physicochemical properties, proximate and nutritional composition of Gracilaria edulis collected from Palk Bay.
25529653	0	0	theme	edulis	94:99	arg1	composition					68:78	nutritional composition	56:78	nutritional composition	56:78	Evaluation of physicochemical properties, proximate and nutritional composition of Gracilaria edulis collected from Palk Bay.
25529653	4	1	theme	linolenic	780:788	arg1	acid					790:793	linolenic acid	780:793	linolenic acid (2.56%)	780:801	G. edulis contains biologically important fatty acids like palmitic acid (2.06%), linolenic acid (2.56%), and oleic acid (1.98%).
25529653	4	1	theme	linolenic	780:788	arg1	%					800:800	2.56%	796:800	2.56%	796:800	G. edulis contains biologically important fatty acids like palmitic acid (2.06%), linolenic acid (2.56%), and oleic acid (1.98%).
25529653	2	2	theme	gelling	378:384	arg1	galactans					306:314	galactans	306:314	galactans	306:314	FT-IR analysis of soluble polysaccharides revealed the presence of galactans, 3,6-anhydro-α-L-galactopyranose, sulphated galactose and the gelling agent agar, with the sulphate content estimated as 51.01 μg/mg of polysaccharide.
25529653	2	2	theme	gelling	378:384	arg1	agar					392:395	the gelling agent agar	374:395	the gelling agent agar	374:395	FT-IR analysis of soluble polysaccharides revealed the presence of galactans, 3,6-anhydro-α-L-galactopyranose, sulphated galactose and the gelling agent agar, with the sulphate content estimated as 51.01 μg/mg of polysaccharide.
25529653	2	3	with	galactans	306:314	arg1	content					416:422	the sulphate content	403:422	the sulphate content estimated as 51.01 μg/mg of polysaccharide	403:465	FT-IR analysis of soluble polysaccharides revealed the presence of galactans, 3,6-anhydro-α-L-galactopyranose, sulphated galactose and the gelling agent agar, with the sulphate content estimated as 51.01 μg/mg of polysaccharide.
25529653	0	4	theme	Gracilaria	83:92	arg1	edulis					94:99	Gracilaria edulis	83:99	Gracilaria edulis	83:99	Evaluation of physicochemical properties, proximate and nutritional composition of Gracilaria edulis collected from Palk Bay.
25529653	3	5	theme	mg/g	649:652	arg1	protein					628:634	crude protein	622:634	crude protein (6.68 ± 0.94 mg/g DW)	622:656	Results of physicochemical properties and nutritional profile reveal the presence of dietary fibre (8.9 ± 0.62% DW), carbohydrate (101.61 ± 1.8 mg/g DW), crude protein (6.68 ± 0.94 mg/g DW) and lipid content (8.3 ± 1.03 mg/g DW).
25529653	3	5	theme	mg/g	649:652	arg1	DW					654:655	6.68 ± 0.94 mg/g DW	637:655	6.68 ± 0.94 mg/g DW	637:655	Results of physicochemical properties and nutritional profile reveal the presence of dietary fibre (8.9 ± 0.62% DW), carbohydrate (101.61 ± 1.8 mg/g DW), crude protein (6.68 ± 0.94 mg/g DW) and lipid content (8.3 ± 1.03 mg/g DW).
25529653	1	6	theme	present	156:162	arg1	edulis					137:142	Gracilaria edulis	126:142	Gracilaria edulis	126:142	Gracilaria edulis, a red alga present in southeast coast of India was evaluated for its nutritional composition.
25529653	1	6	theme	present	156:162	arg1	alga					151:154	a red alga	145:154	a red alga present in southeast coast of India	145:190	Gracilaria edulis, a red alga present in southeast coast of India was evaluated for its nutritional composition.
25529653	6	7	used	used	1055:1058	arg2	source					1065:1070	a source	1063:1070	a source of nutrients for human and animals	1063:1105	These findings suggest that G. edulis has potent nutritional value which might be used as a source of nutrients for human and animals.
25529653	6	7	used	used	1055:1058	arg2	value					1034:1038	potent nutritional value	1015:1038	potent nutritional value which might be used as a source of nutrients for human and animals	1015:1105	These findings suggest that G. edulis has potent nutritional value which might be used as a source of nutrients for human and animals.
25529653	5	8	attach	present	861:867	arg2	components					850:859	The other nutritional components	828:859	The other nutritional components present in high amounts	828:883	The other nutritional components present in high amounts are proline, chlorophyll A and B, all the essential amino acids and vitamin A, E and C.
25529653	5	8	attach	present	861:867	arg2	proline					889:895	proline	889:895	proline	889:895	The other nutritional components present in high amounts are proline, chlorophyll A and B, all the essential amino acids and vitamin A, E and C.
25529653	5	8	attach	present	861:867	arg1	amounts					877:883	high amounts	872:883	high amounts	872:883	The other nutritional components present in high amounts are proline, chlorophyll A and B, all the essential amino acids and vitamin A, E and C.
25529653	3	9	dep	%	578:578	arg1	±					572:572	±	572:572	±	572:572	Results of physicochemical properties and nutritional profile reveal the presence of dietary fibre (8.9 ± 0.62% DW), carbohydrate (101.61 ± 1.8 mg/g DW), crude protein (6.68 ± 0.94 mg/g DW) and lipid content (8.3 ± 1.03 mg/g DW).
25529653	1	10	from	present	156:162	arg1	coast					177:181	southeast coast	167:181	southeast coast of India	167:190	Gracilaria edulis, a red alga present in southeast coast of India was evaluated for its nutritional composition.
25529653	3	11	theme	8.9	568:570	arg1	±					572:572	±	572:572	±	572:572	Results of physicochemical properties and nutritional profile reveal the presence of dietary fibre (8.9 ± 0.62% DW), carbohydrate (101.61 ± 1.8 mg/g DW), crude protein (6.68 ± 0.94 mg/g DW) and lipid content (8.3 ± 1.03 mg/g DW).
25529653	5	12	theme	other	832:836	arg1	proline					889:895	proline	889:895	proline	889:895	The other nutritional components present in high amounts are proline, chlorophyll A and B, all the essential amino acids and vitamin A, E and C.
25529653	5	12	theme	other	832:836	arg1	components					850:859	The other nutritional components	828:859	The other nutritional components present in high amounts	828:883	The other nutritional components present in high amounts are proline, chlorophyll A and B, all the essential amino acids and vitamin A, E and C.
25529653	3	13	dep	DW	580:581	arg1	%					578:578	%	578:578	%	578:578	Results of physicochemical properties and nutritional profile reveal the presence of dietary fibre (8.9 ± 0.62% DW), carbohydrate (101.61 ± 1.8 mg/g DW), crude protein (6.68 ± 0.94 mg/g DW) and lipid content (8.3 ± 1.03 mg/g DW).
25529653	3	14	theme	nutritional	510:520	arg1	profile					522:528	nutritional profile	510:528	nutritional profile	510:528	Results of physicochemical properties and nutritional profile reveal the presence of dietary fibre (8.9 ± 0.62% DW), carbohydrate (101.61 ± 1.8 mg/g DW), crude protein (6.68 ± 0.94 mg/g DW) and lipid content (8.3 ± 1.03 mg/g DW).
25529653	6	15	theme	nutrients	1075:1083	arg1	value					1034:1038	potent nutritional value	1015:1038	potent nutritional value which might be used as a source of nutrients for human and animals	1015:1105	These findings suggest that G. edulis has potent nutritional value which might be used as a source of nutrients for human and animals.
25529653	6	15	theme	nutrients	1075:1083	arg1	source					1065:1070	a source	1063:1070	a source of nutrients for human and animals	1063:1105	These findings suggest that G. edulis has potent nutritional value which might be used as a source of nutrients for human and animals.
25529653	3	16	theme	fibre	561:565	arg1	presence					541:548	the presence	537:548	the presence of dietary fibre (8.9 ± 0.62% DW), carbohydrate (101.61 ± 1.8 mg/g DW), crude protein (6.68 ± 0.94 mg/g DW) and lipid content (8.3 ± 1.03 mg/g DW)	537:695	Results of physicochemical properties and nutritional profile reveal the presence of dietary fibre (8.9 ± 0.62% DW), carbohydrate (101.61 ± 1.8 mg/g DW), crude protein (6.68 ± 0.94 mg/g DW) and lipid content (8.3 ± 1.03 mg/g DW).
25529653	5	17	from	amounts	877:883	arg1	present					861:867	present	861:867	present	861:867	The other nutritional components present in high amounts are proline, chlorophyll A and B, all the essential amino acids and vitamin A, E and C.
25529653	2	18	theme	sulphate	407:414	arg1	content					416:422	the sulphate content	403:422	the sulphate content estimated as 51.01 μg/mg of polysaccharide	403:465	FT-IR analysis of soluble polysaccharides revealed the presence of galactans, 3,6-anhydro-α-L-galactopyranose, sulphated galactose and the gelling agent agar, with the sulphate content estimated as 51.01 μg/mg of polysaccharide.
25529653	1	19	attach	present	156:162	arg1	coast					177:181	southeast coast	167:181	southeast coast of India	167:190	Gracilaria edulis, a red alga present in southeast coast of India was evaluated for its nutritional composition.
25529653	1	19	attach	present	156:162	arg2	alga					151:154	a red alga	145:154	a red alga present in southeast coast of India	145:190	Gracilaria edulis, a red alga present in southeast coast of India was evaluated for its nutritional composition.
25529653	1	19	attach	present	156:162	arg2	edulis					137:142	Gracilaria edulis	126:142	Gracilaria edulis	126:142	Gracilaria edulis, a red alga present in southeast coast of India was evaluated for its nutritional composition.
25529653	2	20	theme	polysaccharides	265:279	arg1	analysis					245:252	FT-IR analysis	239:252	FT-IR analysis of soluble polysaccharides	239:279	FT-IR analysis of soluble polysaccharides revealed the presence of galactans, 3,6-anhydro-α-L-galactopyranose, sulphated galactose and the gelling agent agar, with the sulphate content estimated as 51.01 μg/mg of polysaccharide.
25529653	5	21	from	present	861:867	arg1	amounts					877:883	high amounts	872:883	high amounts	872:883	The other nutritional components present in high amounts are proline, chlorophyll A and B, all the essential amino acids and vitamin A, E and C.
25529653	1	22	theme	southeast	167:175	arg1	coast					177:181	southeast coast	167:181	southeast coast of India	167:190	Gracilaria edulis, a red alga present in southeast coast of India was evaluated for its nutritional composition.
25529653	5	23	theme	high	872:875	arg1	amounts					877:883	high amounts	872:883	high amounts	872:883	The other nutritional components present in high amounts are proline, chlorophyll A and B, all the essential amino acids and vitamin A, E and C.
25529653	2	24	theme	polysaccharide	452:465	arg1	μg/mg					443:447	51.01 μg/mg	437:447	51.01 μg/mg of polysaccharide	437:465	FT-IR analysis of soluble polysaccharides revealed the presence of galactans, 3,6-anhydro-α-L-galactopyranose, sulphated galactose and the gelling agent agar, with the sulphate content estimated as 51.01 μg/mg of polysaccharide.
25529653	3	25	theme	mg/g	688:691	arg1	content					668:674	lipid content	662:674	lipid content (8.3 ± 1.03 mg/g DW)	662:695	Results of physicochemical properties and nutritional profile reveal the presence of dietary fibre (8.9 ± 0.62% DW), carbohydrate (101.61 ± 1.8 mg/g DW), crude protein (6.68 ± 0.94 mg/g DW) and lipid content (8.3 ± 1.03 mg/g DW).
25529653	3	25	theme	mg/g	688:691	arg1	DW					693:694	8.3 ± 1.03 mg/g DW	677:694	8.3 ± 1.03 mg/g DW	677:694	Results of physicochemical properties and nutritional profile reveal the presence of dietary fibre (8.9 ± 0.62% DW), carbohydrate (101.61 ± 1.8 mg/g DW), crude protein (6.68 ± 0.94 mg/g DW) and lipid content (8.3 ± 1.03 mg/g DW).
25529653	0	26	theme	properties	30:39	arg1	proximate					42:50	proximate	42:50	proximate	42:50	Evaluation of physicochemical properties, proximate and nutritional composition of Gracilaria edulis collected from Palk Bay.
25529653	0	26	theme	properties	30:39	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of physicochemical properties	0:39	Evaluation of physicochemical properties, proximate and nutritional composition of Gracilaria edulis collected from Palk Bay.
25529653	0	26	theme	properties	30:39	arg1	composition					68:78	nutritional composition	56:78	nutritional composition	56:78	Evaluation of physicochemical properties, proximate and nutritional composition of Gracilaria edulis collected from Palk Bay.
25529653	4	27	theme	fatty	740:744	arg1	acids					746:750	biologically important fatty acids	717:750	biologically important fatty acids	717:750	G. edulis contains biologically important fatty acids like palmitic acid (2.06%), linolenic acid (2.56%), and oleic acid (1.98%).
25529653	4	28	theme	oleic	808:812	arg1	acid					814:817	oleic acid	808:817	oleic acid (1.98%)	808:825	G. edulis contains biologically important fatty acids like palmitic acid (2.06%), linolenic acid (2.56%), and oleic acid (1.98%).
25529653	4	28	theme	oleic	808:812	arg1	%					824:824	1.98%	820:824	1.98%	820:824	G. edulis contains biologically important fatty acids like palmitic acid (2.06%), linolenic acid (2.56%), and oleic acid (1.98%).
25529653	5	29	theme	chlorophyll	898:908	arg1	proline					889:895	proline	889:895	proline	889:895	The other nutritional components present in high amounts are proline, chlorophyll A and B, all the essential amino acids and vitamin A, E and C.
25529653	5	29	theme	chlorophyll	898:908	arg1	A					910:910	chlorophyll A	898:910	chlorophyll A	898:910	The other nutritional components present in high amounts are proline, chlorophyll A and B, all the essential amino acids and vitamin A, E and C.
25529653	5	29	theme	chlorophyll	898:908	arg1	acids					943:947	all the essential amino acids	919:947	all the essential amino acids	919:947	The other nutritional components present in high amounts are proline, chlorophyll A and B, all the essential amino acids and vitamin A, E and C.
25529653	2	30	theme	galactans	306:314	arg1	presence					294:301	the presence	290:301	the presence of galactans, 3,6-anhydro-α-L-galactopyranose, sulphated galactose and the gelling agent agar, with the sulphate content estimated as 51.01 μg/mg of polysaccharide	290:465	FT-IR analysis of soluble polysaccharides revealed the presence of galactans, 3,6-anhydro-α-L-galactopyranose, sulphated galactose and the gelling agent agar, with the sulphate content estimated as 51.01 μg/mg of polysaccharide.
25529653	1	31	theme	India	186:190	arg1	coast					177:181	southeast coast	167:181	southeast coast of India	167:190	Gracilaria edulis, a red alga present in southeast coast of India was evaluated for its nutritional composition.
25529653	0	32	theme	physicochemical	14:28	arg1	properties					30:39	physicochemical properties	14:39	physicochemical properties	14:39	Evaluation of physicochemical properties, proximate and nutritional composition of Gracilaria edulis collected from Palk Bay.
25529653	3	33	theme	±	681:681	arg1	content					668:674	lipid content	662:674	lipid content (8.3 ± 1.03 mg/g DW)	662:695	Results of physicochemical properties and nutritional profile reveal the presence of dietary fibre (8.9 ± 0.62% DW), carbohydrate (101.61 ± 1.8 mg/g DW), crude protein (6.68 ± 0.94 mg/g DW) and lipid content (8.3 ± 1.03 mg/g DW).
25529653	3	33	theme	±	681:681	arg1	DW					693:694	8.3 ± 1.03 mg/g DW	677:694	8.3 ± 1.03 mg/g DW	677:694	Results of physicochemical properties and nutritional profile reveal the presence of dietary fibre (8.9 ± 0.62% DW), carbohydrate (101.61 ± 1.8 mg/g DW), crude protein (6.68 ± 0.94 mg/g DW) and lipid content (8.3 ± 1.03 mg/g DW).
25529653	5	34	theme	nutritional	838:848	arg1	proline					889:895	proline	889:895	proline	889:895	The other nutritional components present in high amounts are proline, chlorophyll A and B, all the essential amino acids and vitamin A, E and C.
25529653	5	34	theme	nutritional	838:848	arg1	components					850:859	The other nutritional components	828:859	The other nutritional components present in high amounts	828:883	The other nutritional components present in high amounts are proline, chlorophyll A and B, all the essential amino acids and vitamin A, E and C.
25529653	3	35	theme	crude	622:626	arg1	fibre					561:565	fibre	561:565	fibre	561:565	Results of physicochemical properties and nutritional profile reveal the presence of dietary fibre (8.9 ± 0.62% DW), carbohydrate (101.61 ± 1.8 mg/g DW), crude protein (6.68 ± 0.94 mg/g DW) and lipid content (8.3 ± 1.03 mg/g DW).
25529653	3	35	theme	crude	622:626	arg1	protein					628:634	crude protein	622:634	crude protein (6.68 ± 0.94 mg/g DW)	622:656	Results of physicochemical properties and nutritional profile reveal the presence of dietary fibre (8.9 ± 0.62% DW), carbohydrate (101.61 ± 1.8 mg/g DW), crude protein (6.68 ± 0.94 mg/g DW) and lipid content (8.3 ± 1.03 mg/g DW).
25529653	3	35	theme	crude	622:626	arg1	DW					654:655	6.68 ± 0.94 mg/g DW	637:655	6.68 ± 0.94 mg/g DW	637:655	Results of physicochemical properties and nutritional profile reveal the presence of dietary fibre (8.9 ± 0.62% DW), carbohydrate (101.61 ± 1.8 mg/g DW), crude protein (6.68 ± 0.94 mg/g DW) and lipid content (8.3 ± 1.03 mg/g DW).
25529653	5	36	theme	vitamin	953:959	arg1	proline					889:895	proline	889:895	proline	889:895	The other nutritional components present in high amounts are proline, chlorophyll A and B, all the essential amino acids and vitamin A, E and C.
25529653	5	36	theme	vitamin	953:959	arg1	A					961:961	vitamin A	953:961	vitamin A	953:961	The other nutritional components present in high amounts are proline, chlorophyll A and B, all the essential amino acids and vitamin A, E and C.
25529653	6	37	theme	nutritional	1022:1032	arg1	value					1034:1038	potent nutritional value	1015:1038	potent nutritional value which might be used as a source of nutrients for human and animals	1015:1105	These findings suggest that G. edulis has potent nutritional value which might be used as a source of nutrients for human and animals.
25529653	6	37	theme	nutritional	1022:1032	arg1	source					1065:1070	a source	1063:1070	a source of nutrients for human and animals	1063:1105	These findings suggest that G. edulis has potent nutritional value which might be used as a source of nutrients for human and animals.
25529653	3	38	theme	properties	495:504	arg1	Results					468:474	Results	468:474	Results of physicochemical properties and nutritional profile	468:528	Results of physicochemical properties and nutritional profile reveal the presence of dietary fibre (8.9 ± 0.62% DW), carbohydrate (101.61 ± 1.8 mg/g DW), crude protein (6.68 ± 0.94 mg/g DW) and lipid content (8.3 ± 1.03 mg/g DW).
25529653	6	39	theme	potent	1015:1020	arg1	value					1034:1038	potent nutritional value	1015:1038	potent nutritional value which might be used as a source of nutrients for human and animals	1015:1105	These findings suggest that G. edulis has potent nutritional value which might be used as a source of nutrients for human and animals.
25529653	6	39	theme	potent	1015:1020	arg1	source					1065:1070	a source	1063:1070	a source of nutrients for human and animals	1063:1105	These findings suggest that G. edulis has potent nutritional value which might be used as a source of nutrients for human and animals.
25529653	5	40	theme	essential	927:935	arg1	acids					943:947	all the essential amino acids	919:947	all the essential amino acids	919:947	The other nutritional components present in high amounts are proline, chlorophyll A and B, all the essential amino acids and vitamin A, E and C.
25529653	5	40	theme	essential	927:935	arg1	A					910:910	chlorophyll A	898:910	chlorophyll A	898:910	The other nutritional components present in high amounts are proline, chlorophyll A and B, all the essential amino acids and vitamin A, E and C.
25529653	0	41	theme	nutritional	56:66	arg1	composition					68:78	nutritional composition	56:78	nutritional composition	56:78	Evaluation of physicochemical properties, proximate and nutritional composition of Gracilaria edulis collected from Palk Bay.
25529653	5	42	theme	amino	937:941	arg1	acids					943:947	all the essential amino acids	919:947	all the essential amino acids	919:947	The other nutritional components present in high amounts are proline, chlorophyll A and B, all the essential amino acids and vitamin A, E and C.
25529653	5	42	theme	amino	937:941	arg1	A					910:910	chlorophyll A	898:910	chlorophyll A	898:910	The other nutritional components present in high amounts are proline, chlorophyll A and B, all the essential amino acids and vitamin A, E and C.
25529653	3	43	theme	lipid	662:666	arg1	content					668:674	lipid content	662:674	lipid content (8.3 ± 1.03 mg/g DW)	662:695	Results of physicochemical properties and nutritional profile reveal the presence of dietary fibre (8.9 ± 0.62% DW), carbohydrate (101.61 ± 1.8 mg/g DW), crude protein (6.68 ± 0.94 mg/g DW) and lipid content (8.3 ± 1.03 mg/g DW).
25529653	3	43	theme	lipid	662:666	arg1	fibre					561:565	fibre	561:565	fibre	561:565	Results of physicochemical properties and nutritional profile reveal the presence of dietary fibre (8.9 ± 0.62% DW), carbohydrate (101.61 ± 1.8 mg/g DW), crude protein (6.68 ± 0.94 mg/g DW) and lipid content (8.3 ± 1.03 mg/g DW).
25529653	3	43	theme	lipid	662:666	arg1	DW					693:694	8.3 ± 1.03 mg/g DW	677:694	8.3 ± 1.03 mg/g DW	677:694	Results of physicochemical properties and nutritional profile reveal the presence of dietary fibre (8.9 ± 0.62% DW), carbohydrate (101.61 ± 1.8 mg/g DW), crude protein (6.68 ± 0.94 mg/g DW) and lipid content (8.3 ± 1.03 mg/g DW).
25529653	3	44	theme	profile	522:528	arg1	Results					468:474	Results	468:474	Results of physicochemical properties and nutritional profile	468:528	Results of physicochemical properties and nutritional profile reveal the presence of dietary fibre (8.9 ± 0.62% DW), carbohydrate (101.61 ± 1.8 mg/g DW), crude protein (6.68 ± 0.94 mg/g DW) and lipid content (8.3 ± 1.03 mg/g DW).
25529653	3	45	theme	±	642:642	arg1	protein					628:634	crude protein	622:634	crude protein (6.68 ± 0.94 mg/g DW)	622:656	Results of physicochemical properties and nutritional profile reveal the presence of dietary fibre (8.9 ± 0.62% DW), carbohydrate (101.61 ± 1.8 mg/g DW), crude protein (6.68 ± 0.94 mg/g DW) and lipid content (8.3 ± 1.03 mg/g DW).
25529653	3	45	theme	±	642:642	arg1	DW					654:655	6.68 ± 0.94 mg/g DW	637:655	6.68 ± 0.94 mg/g DW	637:655	Results of physicochemical properties and nutritional profile reveal the presence of dietary fibre (8.9 ± 0.62% DW), carbohydrate (101.61 ± 1.8 mg/g DW), crude protein (6.68 ± 0.94 mg/g DW) and lipid content (8.3 ± 1.03 mg/g DW).
25529653	4	46	theme	palmitic	757:764	arg1	%					776:776	2.06%	772:776	2.06%	772:776	G. edulis contains biologically important fatty acids like palmitic acid (2.06%), linolenic acid (2.56%), and oleic acid (1.98%).
25529653	4	46	theme	palmitic	757:764	arg1	acid					766:769	palmitic acid	757:769	palmitic acid (2.06%)	757:777	G. edulis contains biologically important fatty acids like palmitic acid (2.06%), linolenic acid (2.56%), and oleic acid (1.98%).
25529653	1	47	theme	Gracilaria	126:135	arg1	edulis					137:142	Gracilaria edulis	126:142	Gracilaria edulis	126:142	Gracilaria edulis, a red alga present in southeast coast of India was evaluated for its nutritional composition.
25529653	1	47	theme	Gracilaria	126:135	arg1	alga					151:154	a red alga	145:154	a red alga present in southeast coast of India	145:190	Gracilaria edulis, a red alga present in southeast coast of India was evaluated for its nutritional composition.
25529653	4	48	theme	important	730:738	arg1	acids					746:750	biologically important fatty acids	717:750	biologically important fatty acids	717:750	G. edulis contains biologically important fatty acids like palmitic acid (2.06%), linolenic acid (2.56%), and oleic acid (1.98%).
25529653	3	49	theme	mg/g	612:615	arg1	carbohydrate					585:596	carbohydrate	585:596	carbohydrate (101.61 ± 1.8 mg/g DW)	585:619	Results of physicochemical properties and nutritional profile reveal the presence of dietary fibre (8.9 ± 0.62% DW), carbohydrate (101.61 ± 1.8 mg/g DW), crude protein (6.68 ± 0.94 mg/g DW) and lipid content (8.3 ± 1.03 mg/g DW).
25529653	3	49	theme	mg/g	612:615	arg1	DW					617:618	101.61 ± 1.8 mg/g DW	599:618	101.61 ± 1.8 mg/g DW	599:618	Results of physicochemical properties and nutritional profile reveal the presence of dietary fibre (8.9 ± 0.62% DW), carbohydrate (101.61 ± 1.8 mg/g DW), crude protein (6.68 ± 0.94 mg/g DW) and lipid content (8.3 ± 1.03 mg/g DW).
25529653	2	50	theme	soluble	257:263	arg1	polysaccharides					265:279	soluble polysaccharides	257:279	soluble polysaccharides	257:279	FT-IR analysis of soluble polysaccharides revealed the presence of galactans, 3,6-anhydro-α-L-galactopyranose, sulphated galactose and the gelling agent agar, with the sulphate content estimated as 51.01 μg/mg of polysaccharide.
25529653	2	51	theme	sulphated	350:358	arg1	galactose					360:368	sulphated galactose	350:368	sulphated galactose	350:368	FT-IR analysis of soluble polysaccharides revealed the presence of galactans, 3,6-anhydro-α-L-galactopyranose, sulphated galactose and the gelling agent agar, with the sulphate content estimated as 51.01 μg/mg of polysaccharide.
25529653	2	51	theme	sulphated	350:358	arg1	galactans					306:314	galactans	306:314	galactans	306:314	FT-IR analysis of soluble polysaccharides revealed the presence of galactans, 3,6-anhydro-α-L-galactopyranose, sulphated galactose and the gelling agent agar, with the sulphate content estimated as 51.01 μg/mg of polysaccharide.
25529653	3	52	theme	0.62	574:577	arg1	±					572:572	±	572:572	±	572:572	Results of physicochemical properties and nutritional profile reveal the presence of dietary fibre (8.9 ± 0.62% DW), carbohydrate (101.61 ± 1.8 mg/g DW), crude protein (6.68 ± 0.94 mg/g DW) and lipid content (8.3 ± 1.03 mg/g DW).
25529653	4	53	theme	G.	698:699	arg1	edulis					701:706	G. edulis	698:706	G. edulis	698:706	G. edulis contains biologically important fatty acids like palmitic acid (2.06%), linolenic acid (2.56%), and oleic acid (1.98%).
25529653	1	54	from	coast	177:181	arg1	present					156:162	present	156:162	present	156:162	Gracilaria edulis, a red alga present in southeast coast of India was evaluated for its nutritional composition.
25529653	1	55	theme	red	147:149	arg1	edulis					137:142	Gracilaria edulis	126:142	Gracilaria edulis	126:142	Gracilaria edulis, a red alga present in southeast coast of India was evaluated for its nutritional composition.
25529653	1	55	theme	red	147:149	arg1	alga					151:154	a red alga	145:154	a red alga present in southeast coast of India	145:190	Gracilaria edulis, a red alga present in southeast coast of India was evaluated for its nutritional composition.
25529653	1	56	theme	nutritional	214:224	arg1	composition					226:236	its nutritional composition	210:236	its nutritional composition	210:236	Gracilaria edulis, a red alga present in southeast coast of India was evaluated for its nutritional composition.
25529653	3	57	theme	±	606:606	arg1	carbohydrate					585:596	carbohydrate	585:596	carbohydrate (101.61 ± 1.8 mg/g DW)	585:619	Results of physicochemical properties and nutritional profile reveal the presence of dietary fibre (8.9 ± 0.62% DW), carbohydrate (101.61 ± 1.8 mg/g DW), crude protein (6.68 ± 0.94 mg/g DW) and lipid content (8.3 ± 1.03 mg/g DW).
25529653	3	57	theme	±	606:606	arg1	DW					617:618	101.61 ± 1.8 mg/g DW	599:618	101.61 ± 1.8 mg/g DW	599:618	Results of physicochemical properties and nutritional profile reveal the presence of dietary fibre (8.9 ± 0.62% DW), carbohydrate (101.61 ± 1.8 mg/g DW), crude protein (6.68 ± 0.94 mg/g DW) and lipid content (8.3 ± 1.03 mg/g DW).
25529653	3	58	theme	physicochemical	479:493	arg1	properties					495:504	physicochemical properties	479:504	physicochemical properties	479:504	Results of physicochemical properties and nutritional profile reveal the presence of dietary fibre (8.9 ± 0.62% DW), carbohydrate (101.61 ± 1.8 mg/g DW), crude protein (6.68 ± 0.94 mg/g DW) and lipid content (8.3 ± 1.03 mg/g DW).
25529653	6	59	theme	G.	1001:1002	arg1	edulis					1004:1009	G. edulis	1001:1009	G. edulis	1001:1009	These findings suggest that G. edulis has potent nutritional value which might be used as a source of nutrients for human and animals.
25529653	4	60	contain	contains	708:715	arg2	acids					746:750	biologically important fatty acids	717:750	biologically important fatty acids	717:750	G. edulis contains biologically important fatty acids like palmitic acid (2.06%), linolenic acid (2.56%), and oleic acid (1.98%).
25529653	4	60	contain	contains	708:715	arg1	edulis					701:706	G. edulis	698:706	G. edulis	698:706	G. edulis contains biologically important fatty acids like palmitic acid (2.06%), linolenic acid (2.56%), and oleic acid (1.98%).
25529653	5	61	theme	present	861:867	arg1	proline					889:895	proline	889:895	proline	889:895	The other nutritional components present in high amounts are proline, chlorophyll A and B, all the essential amino acids and vitamin A, E and C.
25529653	5	61	theme	present	861:867	arg1	components					850:859	The other nutritional components	828:859	The other nutritional components present in high amounts	828:883	The other nutritional components present in high amounts are proline, chlorophyll A and B, all the essential amino acids and vitamin A, E and C.
25529653	2	62	theme	FT-IR	239:243	arg1	analysis					245:252	FT-IR analysis	239:252	FT-IR analysis of soluble polysaccharides	239:279	FT-IR analysis of soluble polysaccharides revealed the presence of galactans, 3,6-anhydro-α-L-galactopyranose, sulphated galactose and the gelling agent agar, with the sulphate content estimated as 51.01 μg/mg of polysaccharide.
25529653	2	63	theme	agent	386:390	arg1	galactans					306:314	galactans	306:314	galactans	306:314	FT-IR analysis of soluble polysaccharides revealed the presence of galactans, 3,6-anhydro-α-L-galactopyranose, sulphated galactose and the gelling agent agar, with the sulphate content estimated as 51.01 μg/mg of polysaccharide.
25529653	2	63	theme	agent	386:390	arg1	agar					392:395	the gelling agent agar	374:395	the gelling agent agar	374:395	FT-IR analysis of soluble polysaccharides revealed the presence of galactans, 3,6-anhydro-α-L-galactopyranose, sulphated galactose and the gelling agent agar, with the sulphate content estimated as 51.01 μg/mg of polysaccharide.
25529653	6	64	contain	has	1011:1013	arg1	edulis					1004:1009	G. edulis	1001:1009	G. edulis	1001:1009	These findings suggest that G. edulis has potent nutritional value which might be used as a source of nutrients for human and animals.
25529653	6	64	contain	has	1011:1013	arg2	source					1065:1070	a source	1063:1070	a source of nutrients for human and animals	1063:1105	These findings suggest that G. edulis has potent nutritional value which might be used as a source of nutrients for human and animals.
25529653	6	64	contain	has	1011:1013	arg2	value					1034:1038	potent nutritional value	1015:1038	potent nutritional value which might be used as a source of nutrients for human and animals	1015:1105	These findings suggest that G. edulis has potent nutritional value which might be used as a source of nutrients for human and animals.
28040662	0	0	theme	iron	114:117	arg1	oxide					119:123	superparamagnetic iron oxide	96:123	superparamagnetic iron oxide	96:123	Development and application of micro-polysaccharide drug carriers incorporating doxorubicin and superparamagnetic iron oxide for bimodality treatment of hepatocellular carcinoma.
28040662	6	1	theme	G2/Huh-6-bearing	1228:1243	arg1	mice					1245:1248	Hep G2/Huh-6-bearing mice	1224:1248	Hep G2/Huh-6-bearing mice model in vivo	1224:1262	According to the results of a cell viability assay and an animal study, the DOX-SPIO-CS/CHI MPs exhibited stronger cytotoxicity than did free DOX when it was administered to Hep G2 and Huh-6 human liver cancer cell lines in vitro and to nude mice of Hep G2/Huh-6-bearing mice model in vivo.
28040662	5	2	theme	MPs	907:909	arg1	behavior					879:886	the sustained-release behavior	857:886	the sustained-release behavior of DOX-SPIO-CS/CHI MPs, which released 51.5% of DOX within 48h of the testing period	857:971	The results of release profile test revealed the sustained-release behavior of DOX-SPIO-CS/CHI MPs, which released 51.5% of DOX within 48h of the testing period.
28040662	1	3	theme	iron	284:287	arg1	CHI					337:339	CHI	337:339	CHI	337:339	In this study, we demonstrated a novel polyelectrolyte microparticle, doxorubicin(DOX)-superparamagnetic iron oxide (SPIO)-chondroitin sulfate (CS)/chitosan (CHI)microparticles (MPs), as a drug delivery system for hepatic cancer treatment.
28040662	1	3	theme	iron	284:287	arg1	/chitosan					326:334	a novel polyelectrolyte microparticle, doxorubicin(DOX)-superparamagnetic iron oxide (SPIO)-chondroitin sulfate (CS)/chitosan	210:334	/chitosan	326:334	In this study, we demonstrated a novel polyelectrolyte microparticle, doxorubicin(DOX)-superparamagnetic iron oxide (SPIO)-chondroitin sulfate (CS)/chitosan (CHI)microparticles (MPs), as a drug delivery system for hepatic cancer treatment.
28040662	0	4	theme	superparamagnetic	96:112	arg1	oxide					119:123	superparamagnetic iron oxide	96:123	superparamagnetic iron oxide	96:123	Development and application of micro-polysaccharide drug carriers incorporating doxorubicin and superparamagnetic iron oxide for bimodality treatment of hepatocellular carcinoma.
28040662	6	5	theme	Hep	1224:1226	arg1	mice					1245:1248	Hep G2/Huh-6-bearing mice	1224:1248	Hep G2/Huh-6-bearing mice model in vivo	1224:1262	According to the results of a cell viability assay and an animal study, the DOX-SPIO-CS/CHI MPs exhibited stronger cytotoxicity than did free DOX when it was administered to Hep G2 and Huh-6 human liver cancer cell lines in vitro and to nude mice of Hep G2/Huh-6-bearing mice model in vivo.
28040662	6	6	theme	DOX-SPIO-CS/CHI	1050:1064	arg1	MPs					1066:1068	the DOX-SPIO-CS/CHI MPs	1046:1068	the DOX-SPIO-CS/CHI MPs	1046:1068	According to the results of a cell viability assay and an animal study, the DOX-SPIO-CS/CHI MPs exhibited stronger cytotoxicity than did free DOX when it was administered to Hep G2 and Huh-6 human liver cancer cell lines in vitro and to nude mice of Hep G2/Huh-6-bearing mice model in vivo.
28040662	1	7	theme	oxide	289:293	arg1	CHI					337:339	CHI	337:339	CHI	337:339	In this study, we demonstrated a novel polyelectrolyte microparticle, doxorubicin(DOX)-superparamagnetic iron oxide (SPIO)-chondroitin sulfate (CS)/chitosan (CHI)microparticles (MPs), as a drug delivery system for hepatic cancer treatment.
28040662	1	7	theme	oxide	289:293	arg1	/chitosan					326:334	a novel polyelectrolyte microparticle, doxorubicin(DOX)-superparamagnetic iron oxide (SPIO)-chondroitin sulfate (CS)/chitosan	210:334	/chitosan	326:334	In this study, we demonstrated a novel polyelectrolyte microparticle, doxorubicin(DOX)-superparamagnetic iron oxide (SPIO)-chondroitin sulfate (CS)/chitosan (CHI)microparticles (MPs), as a drug delivery system for hepatic cancer treatment.
28040662	3	8	theme	spherical	671:679	arg1	shape					681:685	a spherical shape	669:685	a spherical shape	669:685	The results showed that our DOX-SPIO-CS/CHI MPs had an average diameter of 1.43±0.54μm and exhibited a spherical shape.
28040662	6	9	theme	liver	1171:1175	arg1	lines					1189:1193	Huh-6 human liver cancer cell lines	1159:1193	Huh-6 human liver cancer cell lines in vitro	1159:1202	According to the results of a cell viability assay and an animal study, the DOX-SPIO-CS/CHI MPs exhibited stronger cytotoxicity than did free DOX when it was administered to Hep G2 and Huh-6 human liver cancer cell lines in vitro and to nude mice of Hep G2/Huh-6-bearing mice model in vivo.
28040662	2	10	dep	in	533:534	arg1	vitro					536:540	vitro	536:540	vitro	536:540	We also investigated the properties of these microparticles through composition determination, formulation tests, in vitro study, and in vivo study.
28040662	1	11	dep	/chitosan	326:334	arg1	doxorubicin					249:259	a novel polyelectrolyte microparticle, doxorubicin(DOX)-superparamagnetic iron oxide (SPIO)-chondroitin sulfate (CS)/chitosan	210:334	doxorubicin	249:259	In this study, we demonstrated a novel polyelectrolyte microparticle, doxorubicin(DOX)-superparamagnetic iron oxide (SPIO)-chondroitin sulfate (CS)/chitosan (CHI)microparticles (MPs), as a drug delivery system for hepatic cancer treatment.
28040662	6	12	theme	Hep	1148:1150	arg1	G2					1152:1153	Hep G2	1148:1153	Hep G2	1148:1153	According to the results of a cell viability assay and an animal study, the DOX-SPIO-CS/CHI MPs exhibited stronger cytotoxicity than did free DOX when it was administered to Hep G2 and Huh-6 human liver cancer cell lines in vitro and to nude mice of Hep G2/Huh-6-bearing mice model in vivo.
28040662	1	13	theme	SPIO	296:299	arg1	CHI					337:339	CHI	337:339	CHI	337:339	In this study, we demonstrated a novel polyelectrolyte microparticle, doxorubicin(DOX)-superparamagnetic iron oxide (SPIO)-chondroitin sulfate (CS)/chitosan (CHI)microparticles (MPs), as a drug delivery system for hepatic cancer treatment.
28040662	1	13	theme	SPIO	296:299	arg1	/chitosan					326:334	a novel polyelectrolyte microparticle, doxorubicin(DOX)-superparamagnetic iron oxide (SPIO)-chondroitin sulfate (CS)/chitosan	210:334	/chitosan	326:334	In this study, we demonstrated a novel polyelectrolyte microparticle, doxorubicin(DOX)-superparamagnetic iron oxide (SPIO)-chondroitin sulfate (CS)/chitosan (CHI)microparticles (MPs), as a drug delivery system for hepatic cancer treatment.
28040662	2	14	theme	formulation	514:524	arg1	tests					526:530	formulation tests	514:530	formulation tests	514:530	We also investigated the properties of these microparticles through composition determination, formulation tests, in vitro study, and in vivo study.
28040662	6	15	theme	free	1111:1114	arg1	DOX					1116:1118	free DOX	1111:1118	free DOX when it was administered to Hep G2 and Huh-6 human liver cancer cell lines in vitro and to nude mice of Hep G2/Huh-6-bearing mice model in vivo	1111:1262	According to the results of a cell viability assay and an animal study, the DOX-SPIO-CS/CHI MPs exhibited stronger cytotoxicity than did free DOX when it was administered to Hep G2 and Huh-6 human liver cancer cell lines in vitro and to nude mice of Hep G2/Huh-6-bearing mice model in vivo.
28040662	5	16	theme	release	827:833	arg1	test					843:846	release profile test	827:846	release profile test	827:846	The results of release profile test revealed the sustained-release behavior of DOX-SPIO-CS/CHI MPs, which released 51.5% of DOX within 48h of the testing period.
28040662	3	17	contain	had	616:618	arg2	diameter					631:638	an average diameter	620:638	an average diameter of 1.43±0.54μm	620:653	The results showed that our DOX-SPIO-CS/CHI MPs had an average diameter of 1.43±0.54μm and exhibited a spherical shape.
28040662	3	17	contain	had	616:618	arg1	MPs					612:614	our DOX-SPIO-CS/CHI MPs	592:614	our DOX-SPIO-CS/CHI MPs	592:614	The results showed that our DOX-SPIO-CS/CHI MPs had an average diameter of 1.43±0.54μm and exhibited a spherical shape.
28040662	5	18	theme	testing	958:964	arg1	period					966:971	the testing period	954:971	the testing period	954:971	The results of release profile test revealed the sustained-release behavior of DOX-SPIO-CS/CHI MPs, which released 51.5% of DOX within 48h of the testing period.
28040662	6	19	theme	study	1039:1043	arg1	results					991:997	the results	987:997	the results of a cell viability assay and an animal study	987:1043	According to the results of a cell viability assay and an animal study, the DOX-SPIO-CS/CHI MPs exhibited stronger cytotoxicity than did free DOX when it was administered to Hep G2 and Huh-6 human liver cancer cell lines in vitro and to nude mice of Hep G2/Huh-6-bearing mice model in vivo.
28040662	0	20	theme	bimodality	129:138	arg1	treatment					140:148	bimodality treatment	129:148	bimodality treatment of hepatocellular carcinoma	129:176	Development and application of micro-polysaccharide drug carriers incorporating doxorubicin and superparamagnetic iron oxide for bimodality treatment of hepatocellular carcinoma.
28040662	5	21	theme	period	966:971	arg1	48h					947:949	48h	947:949	48h of the testing period	947:971	The results of release profile test revealed the sustained-release behavior of DOX-SPIO-CS/CHI MPs, which released 51.5% of DOX within 48h of the testing period.
28040662	2	22	theme	microparticles	464:477	arg1	properties					444:453	the properties	440:453	the properties of these microparticles	440:477	We also investigated the properties of these microparticles through composition determination, formulation tests, in vitro study, and in vivo study.
28040662	4	23	theme	drug	725:728	arg1	carrier					730:736	this drug carrier	720:736	this drug carrier	720:736	The encapsulation efficiency of this drug carrier was approximately 31±8.07%, according to our spectroscopic determination.
28040662	5	24	theme	sustained-release	861:877	arg1	behavior					879:886	the sustained-release behavior	857:886	the sustained-release behavior of DOX-SPIO-CS/CHI MPs, which released 51.5% of DOX within 48h of the testing period	857:971	The results of release profile test revealed the sustained-release behavior of DOX-SPIO-CS/CHI MPs, which released 51.5% of DOX within 48h of the testing period.
28040662	6	25	theme	nude	1211:1214	arg1	mice					1216:1219	nude mice	1211:1219	nude mice of Hep G2/Huh-6-bearing mice model in vivo	1211:1262	According to the results of a cell viability assay and an animal study, the DOX-SPIO-CS/CHI MPs exhibited stronger cytotoxicity than did free DOX when it was administered to Hep G2 and Huh-6 human liver cancer cell lines in vitro and to nude mice of Hep G2/Huh-6-bearing mice model in vivo.
28040662	5	26	theme	DOX	936:938	arg1	DOX					936:938	DOX	936:938	DOX	936:938	The results of release profile test revealed the sustained-release behavior of DOX-SPIO-CS/CHI MPs, which released 51.5% of DOX within 48h of the testing period.
28040662	5	26	theme	DOX	936:938	arg1	%					931:931	51.5%	927:931	51.5% of DOX	927:938	The results of release profile test revealed the sustained-release behavior of DOX-SPIO-CS/CHI MPs, which released 51.5% of DOX within 48h of the testing period.
28040662	1	27	theme	novel	212:216	arg1	CHI					337:339	CHI	337:339	CHI	337:339	In this study, we demonstrated a novel polyelectrolyte microparticle, doxorubicin(DOX)-superparamagnetic iron oxide (SPIO)-chondroitin sulfate (CS)/chitosan (CHI)microparticles (MPs), as a drug delivery system for hepatic cancer treatment.
28040662	1	27	theme	novel	212:216	arg1	/chitosan					326:334	a novel polyelectrolyte microparticle, doxorubicin(DOX)-superparamagnetic iron oxide (SPIO)-chondroitin sulfate (CS)/chitosan	210:334	/chitosan	326:334	In this study, we demonstrated a novel polyelectrolyte microparticle, doxorubicin(DOX)-superparamagnetic iron oxide (SPIO)-chondroitin sulfate (CS)/chitosan (CHI)microparticles (MPs), as a drug delivery system for hepatic cancer treatment.
28040662	1	28	theme	-chondroitin	301:312	arg1	CHI					337:339	CHI	337:339	CHI	337:339	In this study, we demonstrated a novel polyelectrolyte microparticle, doxorubicin(DOX)-superparamagnetic iron oxide (SPIO)-chondroitin sulfate (CS)/chitosan (CHI)microparticles (MPs), as a drug delivery system for hepatic cancer treatment.
28040662	1	28	theme	-chondroitin	301:312	arg1	/chitosan					326:334	a novel polyelectrolyte microparticle, doxorubicin(DOX)-superparamagnetic iron oxide (SPIO)-chondroitin sulfate (CS)/chitosan	210:334	/chitosan	326:334	In this study, we demonstrated a novel polyelectrolyte microparticle, doxorubicin(DOX)-superparamagnetic iron oxide (SPIO)-chondroitin sulfate (CS)/chitosan (CHI)microparticles (MPs), as a drug delivery system for hepatic cancer treatment.
28040662	0	29	theme	hepatocellular	153:166	arg1	carcinoma					168:176	hepatocellular carcinoma	153:176	hepatocellular carcinoma	153:176	Development and application of micro-polysaccharide drug carriers incorporating doxorubicin and superparamagnetic iron oxide for bimodality treatment of hepatocellular carcinoma.
28040662	6	30	theme	assay	1019:1023	arg1	results					991:997	the results	987:997	the results of a cell viability assay and an animal study	987:1043	According to the results of a cell viability assay and an animal study, the DOX-SPIO-CS/CHI MPs exhibited stronger cytotoxicity than did free DOX when it was administered to Hep G2 and Huh-6 human liver cancer cell lines in vitro and to nude mice of Hep G2/Huh-6-bearing mice model in vivo.
28040662	1	31	theme	polyelectrolyte	218:232	arg1	CHI					337:339	CHI	337:339	CHI	337:339	In this study, we demonstrated a novel polyelectrolyte microparticle, doxorubicin(DOX)-superparamagnetic iron oxide (SPIO)-chondroitin sulfate (CS)/chitosan (CHI)microparticles (MPs), as a drug delivery system for hepatic cancer treatment.
28040662	1	31	theme	polyelectrolyte	218:232	arg1	/chitosan					326:334	a novel polyelectrolyte microparticle, doxorubicin(DOX)-superparamagnetic iron oxide (SPIO)-chondroitin sulfate (CS)/chitosan	210:334	/chitosan	326:334	In this study, we demonstrated a novel polyelectrolyte microparticle, doxorubicin(DOX)-superparamagnetic iron oxide (SPIO)-chondroitin sulfate (CS)/chitosan (CHI)microparticles (MPs), as a drug delivery system for hepatic cancer treatment.
28040662	1	32	theme	sulfate	314:320	arg1	CHI					337:339	CHI	337:339	CHI	337:339	In this study, we demonstrated a novel polyelectrolyte microparticle, doxorubicin(DOX)-superparamagnetic iron oxide (SPIO)-chondroitin sulfate (CS)/chitosan (CHI)microparticles (MPs), as a drug delivery system for hepatic cancer treatment.
28040662	1	32	theme	sulfate	314:320	arg1	/chitosan					326:334	a novel polyelectrolyte microparticle, doxorubicin(DOX)-superparamagnetic iron oxide (SPIO)-chondroitin sulfate (CS)/chitosan	210:334	/chitosan	326:334	In this study, we demonstrated a novel polyelectrolyte microparticle, doxorubicin(DOX)-superparamagnetic iron oxide (SPIO)-chondroitin sulfate (CS)/chitosan (CHI)microparticles (MPs), as a drug delivery system for hepatic cancer treatment.
28040662	3	33	theme	average	623:629	arg1	diameter					631:638	an average diameter	620:638	an average diameter of 1.43±0.54μm	620:653	The results showed that our DOX-SPIO-CS/CHI MPs had an average diameter of 1.43±0.54μm and exhibited a spherical shape.
28040662	4	34	theme	carrier	730:736	arg1	%					763:763	approximately 31±8.07%	742:763	approximately 31±8.07%	742:763	The encapsulation efficiency of this drug carrier was approximately 31±8.07%, according to our spectroscopic determination.
28040662	4	34	theme	carrier	730:736	arg1	efficiency					706:715	The encapsulation efficiency	688:715	The encapsulation efficiency of this drug carrier	688:736	The encapsulation efficiency of this drug carrier was approximately 31±8.07%, according to our spectroscopic determination.
28040662	1	35	theme	microparticle	234:246	arg1	CHI					337:339	CHI	337:339	CHI	337:339	In this study, we demonstrated a novel polyelectrolyte microparticle, doxorubicin(DOX)-superparamagnetic iron oxide (SPIO)-chondroitin sulfate (CS)/chitosan (CHI)microparticles (MPs), as a drug delivery system for hepatic cancer treatment.
28040662	1	35	theme	microparticle	234:246	arg1	/chitosan					326:334	a novel polyelectrolyte microparticle, doxorubicin(DOX)-superparamagnetic iron oxide (SPIO)-chondroitin sulfate (CS)/chitosan	210:334	/chitosan	326:334	In this study, we demonstrated a novel polyelectrolyte microparticle, doxorubicin(DOX)-superparamagnetic iron oxide (SPIO)-chondroitin sulfate (CS)/chitosan (CHI)microparticles (MPs), as a drug delivery system for hepatic cancer treatment.
28040662	0	36	theme	drug	52:55	arg1	carriers					57:64	micro-polysaccharide drug carriers	31:64	micro-polysaccharide drug carriers incorporating doxorubicin and superparamagnetic iron oxide for bimodality treatment of hepatocellular carcinoma	31:176	Development and application of micro-polysaccharide drug carriers incorporating doxorubicin and superparamagnetic iron oxide for bimodality treatment of hepatocellular carcinoma.
28040662	1	37	theme	drug	368:371	arg1	microparticles					341:354	a novel polyelectrolyte microparticle, doxorubicin(DOX)-superparamagnetic iron oxide (SPIO)-chondroitin sulfate (CS)/chitosan (CHI)microparticles	210:354	a novel polyelectrolyte microparticle, doxorubicin(DOX)-superparamagnetic iron oxide (SPIO)-chondroitin sulfate (CS)/chitosan (CHI)microparticles (MPs)	210:360	In this study, we demonstrated a novel polyelectrolyte microparticle, doxorubicin(DOX)-superparamagnetic iron oxide (SPIO)-chondroitin sulfate (CS)/chitosan (CHI)microparticles (MPs), as a drug delivery system for hepatic cancer treatment.
28040662	1	37	theme	drug	368:371	arg1	system					382:387	a drug delivery system	366:387	a drug delivery system for hepatic cancer treatment	366:416	In this study, we demonstrated a novel polyelectrolyte microparticle, doxorubicin(DOX)-superparamagnetic iron oxide (SPIO)-chondroitin sulfate (CS)/chitosan (CHI)microparticles (MPs), as a drug delivery system for hepatic cancer treatment.
28040662	6	38	theme	animal	1032:1037	arg1	study					1039:1043	an animal study	1029:1043	an animal study	1029:1043	According to the results of a cell viability assay and an animal study, the DOX-SPIO-CS/CHI MPs exhibited stronger cytotoxicity than did free DOX when it was administered to Hep G2 and Huh-6 human liver cancer cell lines in vitro and to nude mice of Hep G2/Huh-6-bearing mice model in vivo.
28040662	5	39	theme	profile	835:841	arg1	test					843:846	release profile test	827:846	release profile test	827:846	The results of release profile test revealed the sustained-release behavior of DOX-SPIO-CS/CHI MPs, which released 51.5% of DOX within 48h of the testing period.
28040662	0	40	theme	micro-polysaccharide	31:50	arg1	carriers					57:64	micro-polysaccharide drug carriers	31:64	micro-polysaccharide drug carriers incorporating doxorubicin and superparamagnetic iron oxide for bimodality treatment of hepatocellular carcinoma	31:176	Development and application of micro-polysaccharide drug carriers incorporating doxorubicin and superparamagnetic iron oxide for bimodality treatment of hepatocellular carcinoma.
28040662	1	41	theme	CS	323:324	arg1	CHI					337:339	CHI	337:339	CHI	337:339	In this study, we demonstrated a novel polyelectrolyte microparticle, doxorubicin(DOX)-superparamagnetic iron oxide (SPIO)-chondroitin sulfate (CS)/chitosan (CHI)microparticles (MPs), as a drug delivery system for hepatic cancer treatment.
28040662	1	41	theme	CS	323:324	arg1	/chitosan					326:334	a novel polyelectrolyte microparticle, doxorubicin(DOX)-superparamagnetic iron oxide (SPIO)-chondroitin sulfate (CS)/chitosan	210:334	/chitosan	326:334	In this study, we demonstrated a novel polyelectrolyte microparticle, doxorubicin(DOX)-superparamagnetic iron oxide (SPIO)-chondroitin sulfate (CS)/chitosan (CHI)microparticles (MPs), as a drug delivery system for hepatic cancer treatment.
28040662	0	42	theme	carcinoma	168:176	arg1	treatment					140:148	bimodality treatment	129:148	bimodality treatment of hepatocellular carcinoma	129:176	Development and application of micro-polysaccharide drug carriers incorporating doxorubicin and superparamagnetic iron oxide for bimodality treatment of hepatocellular carcinoma.
28040662	1	43	theme	delivery	373:380	arg1	microparticles					341:354	a novel polyelectrolyte microparticle, doxorubicin(DOX)-superparamagnetic iron oxide (SPIO)-chondroitin sulfate (CS)/chitosan (CHI)microparticles	210:354	a novel polyelectrolyte microparticle, doxorubicin(DOX)-superparamagnetic iron oxide (SPIO)-chondroitin sulfate (CS)/chitosan (CHI)microparticles (MPs)	210:360	In this study, we demonstrated a novel polyelectrolyte microparticle, doxorubicin(DOX)-superparamagnetic iron oxide (SPIO)-chondroitin sulfate (CS)/chitosan (CHI)microparticles (MPs), as a drug delivery system for hepatic cancer treatment.
28040662	1	43	theme	delivery	373:380	arg1	system					382:387	a drug delivery system	366:387	a drug delivery system for hepatic cancer treatment	366:416	In this study, we demonstrated a novel polyelectrolyte microparticle, doxorubicin(DOX)-superparamagnetic iron oxide (SPIO)-chondroitin sulfate (CS)/chitosan (CHI)microparticles (MPs), as a drug delivery system for hepatic cancer treatment.
28040662	2	44	theme	in	533:534	arg1	study					542:546	in vitro study	533:546	in vitro study	533:546	We also investigated the properties of these microparticles through composition determination, formulation tests, in vitro study, and in vivo study.
28040662	5	45	theme	test	843:846	arg1	results					816:822	The results	812:822	The results of release profile test	812:846	The results of release profile test revealed the sustained-release behavior of DOX-SPIO-CS/CHI MPs, which released 51.5% of DOX within 48h of the testing period.
28040662	6	46	theme	viability	1009:1017	arg1	assay					1019:1023	a cell viability assay	1002:1023	a cell viability assay	1002:1023	According to the results of a cell viability assay and an animal study, the DOX-SPIO-CS/CHI MPs exhibited stronger cytotoxicity than did free DOX when it was administered to Hep G2 and Huh-6 human liver cancer cell lines in vitro and to nude mice of Hep G2/Huh-6-bearing mice model in vivo.
28040662	6	47	theme	cell	1184:1187	arg1	lines					1189:1193	Huh-6 human liver cancer cell lines	1159:1193	Huh-6 human liver cancer cell lines in vitro	1159:1202	According to the results of a cell viability assay and an animal study, the DOX-SPIO-CS/CHI MPs exhibited stronger cytotoxicity than did free DOX when it was administered to Hep G2 and Huh-6 human liver cancer cell lines in vitro and to nude mice of Hep G2/Huh-6-bearing mice model in vivo.
28040662	6	48	theme	model	1250:1254	arg1	mice					1245:1248	Hep G2/Huh-6-bearing mice	1224:1248	Hep G2/Huh-6-bearing mice model in vivo	1224:1262	According to the results of a cell viability assay and an animal study, the DOX-SPIO-CS/CHI MPs exhibited stronger cytotoxicity than did free DOX when it was administered to Hep G2 and Huh-6 human liver cancer cell lines in vitro and to nude mice of Hep G2/Huh-6-bearing mice model in vivo.
28040662	4	49	theme	encapsulation	692:704	arg1	%					763:763	approximately 31±8.07%	742:763	approximately 31±8.07%	742:763	The encapsulation efficiency of this drug carrier was approximately 31±8.07%, according to our spectroscopic determination.
28040662	4	49	theme	encapsulation	692:704	arg1	efficiency					706:715	The encapsulation efficiency	688:715	The encapsulation efficiency of this drug carrier	688:736	The encapsulation efficiency of this drug carrier was approximately 31±8.07%, according to our spectroscopic determination.
28040662	0	50	theme	carriers	57:64	arg1	application					16:26	application	16:26	application	16:26	Development and application of micro-polysaccharide drug carriers incorporating doxorubicin and superparamagnetic iron oxide for bimodality treatment of hepatocellular carcinoma.
28040662	0	50	theme	carriers	57:64	arg1	Development					0:10	Development	0:10	Development	0:10	Development and application of micro-polysaccharide drug carriers incorporating doxorubicin and superparamagnetic iron oxide for bimodality treatment of hepatocellular carcinoma.
28040662	1	51	theme	/chitosan	326:334	arg1	microparticles					341:354	a novel polyelectrolyte microparticle, doxorubicin(DOX)-superparamagnetic iron oxide (SPIO)-chondroitin sulfate (CS)/chitosan (CHI)microparticles	210:354	a novel polyelectrolyte microparticle, doxorubicin(DOX)-superparamagnetic iron oxide (SPIO)-chondroitin sulfate (CS)/chitosan (CHI)microparticles (MPs)	210:360	In this study, we demonstrated a novel polyelectrolyte microparticle, doxorubicin(DOX)-superparamagnetic iron oxide (SPIO)-chondroitin sulfate (CS)/chitosan (CHI)microparticles (MPs), as a drug delivery system for hepatic cancer treatment.
28040662	1	51	theme	/chitosan	326:334	arg1	system					382:387	a drug delivery system	366:387	a drug delivery system for hepatic cancer treatment	366:416	In this study, we demonstrated a novel polyelectrolyte microparticle, doxorubicin(DOX)-superparamagnetic iron oxide (SPIO)-chondroitin sulfate (CS)/chitosan (CHI)microparticles (MPs), as a drug delivery system for hepatic cancer treatment.
28040662	1	51	theme	/chitosan	326:334	arg1	MPs					357:359	MPs	357:359	MPs	357:359	In this study, we demonstrated a novel polyelectrolyte microparticle, doxorubicin(DOX)-superparamagnetic iron oxide (SPIO)-chondroitin sulfate (CS)/chitosan (CHI)microparticles (MPs), as a drug delivery system for hepatic cancer treatment.
28040662	6	52	theme	cancer	1177:1182	arg1	lines					1189:1193	Huh-6 human liver cancer cell lines	1159:1193	Huh-6 human liver cancer cell lines in vitro	1159:1202	According to the results of a cell viability assay and an animal study, the DOX-SPIO-CS/CHI MPs exhibited stronger cytotoxicity than did free DOX when it was administered to Hep G2 and Huh-6 human liver cancer cell lines in vitro and to nude mice of Hep G2/Huh-6-bearing mice model in vivo.
28040662	2	53	theme	in	553:554	arg1	study					561:565	in vivo study	553:565	in vivo study	553:565	We also investigated the properties of these microparticles through composition determination, formulation tests, in vitro study, and in vivo study.
28040662	1	54	theme	hepatic	393:399	arg1	treatment					408:416	hepatic cancer treatment	393:416	hepatic cancer treatment	393:416	In this study, we demonstrated a novel polyelectrolyte microparticle, doxorubicin(DOX)-superparamagnetic iron oxide (SPIO)-chondroitin sulfate (CS)/chitosan (CHI)microparticles (MPs), as a drug delivery system for hepatic cancer treatment.
28040662	3	55	theme	DOX-SPIO-CS/CHI	596:610	arg1	MPs					612:614	our DOX-SPIO-CS/CHI MPs	592:614	our DOX-SPIO-CS/CHI MPs	592:614	The results showed that our DOX-SPIO-CS/CHI MPs had an average diameter of 1.43±0.54μm and exhibited a spherical shape.
28040662	6	56	theme	cell	1004:1007	arg1	assay					1019:1023	a cell viability assay	1002:1023	a cell viability assay	1002:1023	According to the results of a cell viability assay and an animal study, the DOX-SPIO-CS/CHI MPs exhibited stronger cytotoxicity than did free DOX when it was administered to Hep G2 and Huh-6 human liver cancer cell lines in vitro and to nude mice of Hep G2/Huh-6-bearing mice model in vivo.
28040662	6	57	theme	mice	1245:1248	arg1	mice					1216:1219	nude mice	1211:1219	nude mice of Hep G2/Huh-6-bearing mice model in vivo	1211:1262	According to the results of a cell viability assay and an animal study, the DOX-SPIO-CS/CHI MPs exhibited stronger cytotoxicity than did free DOX when it was administered to Hep G2 and Huh-6 human liver cancer cell lines in vitro and to nude mice of Hep G2/Huh-6-bearing mice model in vivo.
28040662	1	58	theme	cancer	401:406	arg1	treatment					408:416	hepatic cancer treatment	393:416	hepatic cancer treatment	393:416	In this study, we demonstrated a novel polyelectrolyte microparticle, doxorubicin(DOX)-superparamagnetic iron oxide (SPIO)-chondroitin sulfate (CS)/chitosan (CHI)microparticles (MPs), as a drug delivery system for hepatic cancer treatment.
28040662	6	59	theme	human	1165:1169	arg1	lines					1189:1193	Huh-6 human liver cancer cell lines	1159:1193	Huh-6 human liver cancer cell lines in vitro	1159:1202	According to the results of a cell viability assay and an animal study, the DOX-SPIO-CS/CHI MPs exhibited stronger cytotoxicity than did free DOX when it was administered to Hep G2 and Huh-6 human liver cancer cell lines in vitro and to nude mice of Hep G2/Huh-6-bearing mice model in vivo.
28040662	1	60	theme	DOX	261:263	arg1	CHI					337:339	CHI	337:339	CHI	337:339	In this study, we demonstrated a novel polyelectrolyte microparticle, doxorubicin(DOX)-superparamagnetic iron oxide (SPIO)-chondroitin sulfate (CS)/chitosan (CHI)microparticles (MPs), as a drug delivery system for hepatic cancer treatment.
28040662	1	60	theme	DOX	261:263	arg1	/chitosan					326:334	a novel polyelectrolyte microparticle, doxorubicin(DOX)-superparamagnetic iron oxide (SPIO)-chondroitin sulfate (CS)/chitosan	210:334	/chitosan	326:334	In this study, we demonstrated a novel polyelectrolyte microparticle, doxorubicin(DOX)-superparamagnetic iron oxide (SPIO)-chondroitin sulfate (CS)/chitosan (CHI)microparticles (MPs), as a drug delivery system for hepatic cancer treatment.
28040662	2	61	dep	in	553:554	arg1	vivo					556:559	vivo	556:559	vivo	556:559	We also investigated the properties of these microparticles through composition determination, formulation tests, in vitro study, and in vivo study.
28040662	3	62	theme	1.43±0.54μm	643:653	arg1	diameter					631:638	an average diameter	620:638	an average diameter of 1.43±0.54μm	620:653	The results showed that our DOX-SPIO-CS/CHI MPs had an average diameter of 1.43±0.54μm and exhibited a spherical shape.
28040662	4	63	theme	spectroscopic	783:795	arg1	determination					797:809	our spectroscopic determination	779:809	our spectroscopic determination	779:809	The encapsulation efficiency of this drug carrier was approximately 31±8.07%, according to our spectroscopic determination.
28040662	6	64	theme	stronger	1080:1087	arg1	cytotoxicity					1089:1100	stronger cytotoxicity	1080:1100	stronger cytotoxicity	1080:1100	According to the results of a cell viability assay and an animal study, the DOX-SPIO-CS/CHI MPs exhibited stronger cytotoxicity than did free DOX when it was administered to Hep G2 and Huh-6 human liver cancer cell lines in vitro and to nude mice of Hep G2/Huh-6-bearing mice model in vivo.
28040662	2	65	theme	composition	487:497	arg1	determination					499:511	composition determination	487:511	composition determination	487:511	We also investigated the properties of these microparticles through composition determination, formulation tests, in vitro study, and in vivo study.
28040662	6	66	dep	did	1107:1109	arg1	than					1102:1105	than	1102:1105	than	1102:1105	According to the results of a cell viability assay and an animal study, the DOX-SPIO-CS/CHI MPs exhibited stronger cytotoxicity than did free DOX when it was administered to Hep G2 and Huh-6 human liver cancer cell lines in vitro and to nude mice of Hep G2/Huh-6-bearing mice model in vivo.
28040662	5	67	theme	DOX-SPIO-CS/CHI	891:905	arg1	MPs					907:909	DOX-SPIO-CS/CHI MPs	891:909	DOX-SPIO-CS/CHI MPs	891:909	The results of release profile test revealed the sustained-release behavior of DOX-SPIO-CS/CHI MPs, which released 51.5% of DOX within 48h of the testing period.
28040662	1	68	theme	-superparamagnetic	265:282	arg1	CHI					337:339	CHI	337:339	CHI	337:339	In this study, we demonstrated a novel polyelectrolyte microparticle, doxorubicin(DOX)-superparamagnetic iron oxide (SPIO)-chondroitin sulfate (CS)/chitosan (CHI)microparticles (MPs), as a drug delivery system for hepatic cancer treatment.
28040662	1	68	theme	-superparamagnetic	265:282	arg1	/chitosan					326:334	a novel polyelectrolyte microparticle, doxorubicin(DOX)-superparamagnetic iron oxide (SPIO)-chondroitin sulfate (CS)/chitosan	210:334	/chitosan	326:334	In this study, we demonstrated a novel polyelectrolyte microparticle, doxorubicin(DOX)-superparamagnetic iron oxide (SPIO)-chondroitin sulfate (CS)/chitosan (CHI)microparticles (MPs), as a drug delivery system for hepatic cancer treatment.
25817671	4	0	theme	silica	469:474	arg1	gel					476:478	nanostructured silica gel	454:478	nanostructured silica gel	454:478	In both cases, nanostructured silica gel was in situ formed inside cellulose matrix.
25817671	7	1	theme	mesoporous	913:922	arg1	pores					948:952	superinsulating mesoporous silica inside cellulose pores	897:952	superinsulating mesoporous silica inside cellulose pores	897:952	Thermal conductivity of composite aerogels was lower than that of cellulose aerogel due to the formation of superinsulating mesoporous silica inside cellulose pores.
25817671	3	2	theme	enormous	320:327	arg1	decrease					329:336	an enormous decrease	317:336	an enormous decrease in the impregnation times	317:362	The latter allowed an enormous decrease in the impregnation times, by almost three orders of magnitude, for a sample with the same geometry.
25817671	5	3	theme	cellulose	628:636	arg1	aerogel					638:644	cellulose aerogel	628:644	cellulose aerogel alone to organic-inorganic composite	628:681	Nitrogen adsorption analysis revealed an almost threefold increase in pores specific surface area, from cellulose aerogel alone to organic-inorganic composite.
25817671	5	4	theme	threefold	572:580	arg1	increase					582:589	an almost threefold increase	562:589	an almost threefold increase in pores specific surface area	562:620	Nitrogen adsorption analysis revealed an almost threefold increase in pores specific surface area, from cellulose aerogel alone to organic-inorganic composite.
25817671	5	5	from	increase	582:589	arg1	pores					594:598	pores	594:598	pores specific surface area	594:620	Nitrogen adsorption analysis revealed an almost threefold increase in pores specific surface area, from cellulose aerogel alone to organic-inorganic composite.
25817671	2	6	dep	methods	212:218	arg1	methods					212:218	two methods	208:218	two methods: (i) molecular diffusion and (ii) forced flow induced by pressure difference	208:295	Wet coagulated cellulose was impregnated with silica phase, polyethoxydisiloxane, using two methods: (i) molecular diffusion and (ii) forced flow induced by pressure difference.
25817671	2	6	dep	methods	212:218	arg1	diffusion					235:243	(i) molecular diffusion	221:243	(i) molecular diffusion	221:243	Wet coagulated cellulose was impregnated with silica phase, polyethoxydisiloxane, using two methods: (i) molecular diffusion and (ii) forced flow induced by pressure difference.
25817671	2	6	dep	methods	212:218	arg1	flow					261:264	(ii) forced flow	249:264	(ii) forced flow	249:264	Wet coagulated cellulose was impregnated with silica phase, polyethoxydisiloxane, using two methods: (i) molecular diffusion and (ii) forced flow induced by pressure difference.
25817671	2	7	theme	pressure	277:284	arg1	difference					286:295	pressure difference	277:295	pressure difference	277:295	Wet coagulated cellulose was impregnated with silica phase, polyethoxydisiloxane, using two methods: (i) molecular diffusion and (ii) forced flow induced by pressure difference.
25817671	7	8	theme	due	873:875	arg1	aerogel					865:871	cellulose aerogel	855:871	cellulose aerogel due to the formation of superinsulating mesoporous silica inside cellulose pores	855:952	Thermal conductivity of composite aerogels was lower than that of cellulose aerogel due to the formation of superinsulating mesoporous silica inside cellulose pores.
25817671	5	9	theme	specific	600:607	arg1	pores					594:598	pores	594:598	pores specific surface area	594:620	Nitrogen adsorption analysis revealed an almost threefold increase in pores specific surface area, from cellulose aerogel alone to organic-inorganic composite.
25817671	8	10	theme	reference	1013:1021	arg1	aerogels					1023:1030	reference aerogels	1013:1030	reference aerogels	1013:1030	Furthermore, composite aerogels were stiffer than each of reference aerogels.
25817671	6	11	theme	thermal	696:702	arg1	conductivity					704:715	thermal conductivity	696:715	thermal conductivity	696:715	Morphology, thermal conductivity and mechanical properties under uniaxial compression were investigated.
25817671	3	12	theme	impregnation	345:356	arg1	times					358:362	the impregnation times	341:362	the impregnation times	341:362	The latter allowed an enormous decrease in the impregnation times, by almost three orders of magnitude, for a sample with the same geometry.
25817671	4	13	theme	nanostructured	454:467	arg1	gel					476:478	nanostructured silica gel	454:478	nanostructured silica gel	454:478	In both cases, nanostructured silica gel was in situ formed inside cellulose matrix.
25817671	5	14	theme	surface	609:615	arg1	area					617:620	surface area	609:620	surface area	609:620	Nitrogen adsorption analysis revealed an almost threefold increase in pores specific surface area, from cellulose aerogel alone to organic-inorganic composite.
25817671	2	15	theme	silica	166:171	arg1	phase					173:177	silica phase	166:177	silica phase	166:177	Wet coagulated cellulose was impregnated with silica phase, polyethoxydisiloxane, using two methods: (i) molecular diffusion and (ii) forced flow induced by pressure difference.
25817671	2	15	theme	silica	166:171	arg1	polyethoxydisiloxane					180:199	polyethoxydisiloxane	180:199	polyethoxydisiloxane	180:199	Wet coagulated cellulose was impregnated with silica phase, polyethoxydisiloxane, using two methods: (i) molecular diffusion and (ii) forced flow induced by pressure difference.
25817671	5	16	theme	organic-inorganic	655:671	arg1	composite					673:681	organic-inorganic composite	655:681	organic-inorganic composite	655:681	Nitrogen adsorption analysis revealed an almost threefold increase in pores specific surface area, from cellulose aerogel alone to organic-inorganic composite.
25817671	2	17	dep	flow	261:264	arg1	ii					250:251	ii	250:251	ii	250:251	Wet coagulated cellulose was impregnated with silica phase, polyethoxydisiloxane, using two methods: (i) molecular diffusion and (ii) forced flow induced by pressure difference.
25817671	7	18	theme	cellulose	855:863	arg1	aerogel					865:871	cellulose aerogel	855:871	cellulose aerogel due to the formation of superinsulating mesoporous silica inside cellulose pores	855:952	Thermal conductivity of composite aerogels was lower than that of cellulose aerogel due to the formation of superinsulating mesoporous silica inside cellulose pores.
25817671	2	19	dep	diffusion	235:243	arg1	i					222:222	i	222:222	i	222:222	Wet coagulated cellulose was impregnated with silica phase, polyethoxydisiloxane, using two methods: (i) molecular diffusion and (ii) forced flow induced by pressure difference.
25817671	7	20	theme	composite	813:821	arg1	aerogels					823:830	composite aerogels	813:830	composite aerogels	813:830	Thermal conductivity of composite aerogels was lower than that of cellulose aerogel due to the formation of superinsulating mesoporous silica inside cellulose pores.
25817671	8	21	theme	composite	968:976	arg1	aerogels					978:985	composite aerogels	968:985	composite aerogels	968:985	Furthermore, composite aerogels were stiffer than each of reference aerogels.
25817671	7	22	theme	aerogels	823:830	arg1	conductivity					797:808	Thermal conductivity	789:808	Thermal conductivity of composite aerogels	789:830	Thermal conductivity of composite aerogels was lower than that of cellulose aerogel due to the formation of superinsulating mesoporous silica inside cellulose pores.
25817671	3	23	theme	same	424:427	arg1	geometry					429:436	the same geometry	420:436	the same geometry	420:436	The latter allowed an enormous decrease in the impregnation times, by almost three orders of magnitude, for a sample with the same geometry.
25817671	2	24	theme	coagulated	124:133	arg1	cellulose					135:143	Wet coagulated cellulose	120:143	Wet coagulated cellulose	120:143	Wet coagulated cellulose was impregnated with silica phase, polyethoxydisiloxane, using two methods: (i) molecular diffusion and (ii) forced flow induced by pressure difference.
25817671	5	25	dep	specific	600:607	arg1	area					617:620	surface area	609:620	surface area	609:620	Nitrogen adsorption analysis revealed an almost threefold increase in pores specific surface area, from cellulose aerogel alone to organic-inorganic composite.
25817671	2	26	theme	Wet	120:122	arg1	cellulose					135:143	Wet coagulated cellulose	120:143	Wet coagulated cellulose	120:143	Wet coagulated cellulose was impregnated with silica phase, polyethoxydisiloxane, using two methods: (i) molecular diffusion and (ii) forced flow induced by pressure difference.
25817671	3	27	theme	magnitude	391:399	arg1	orders					381:386	almost three orders	368:386	almost three orders of magnitude	368:399	The latter allowed an enormous decrease in the impregnation times, by almost three orders of magnitude, for a sample with the same geometry.
25817671	4	28	theme	cellulose	506:514	arg1	matrix					516:521	cellulose matrix	506:521	cellulose matrix	506:521	In both cases, nanostructured silica gel was in situ formed inside cellulose matrix.
25817671	3	29	with	sample	408:413	arg1	geometry					429:436	the same geometry	420:436	the same geometry	420:436	The latter allowed an enormous decrease in the impregnation times, by almost three orders of magnitude, for a sample with the same geometry.
25817671	2	30	theme	molecular	225:233	arg1	methods					212:218	two methods	208:218	two methods: (i) molecular diffusion and (ii) forced flow induced by pressure difference	208:295	Wet coagulated cellulose was impregnated with silica phase, polyethoxydisiloxane, using two methods: (i) molecular diffusion and (ii) forced flow induced by pressure difference.
25817671	2	30	theme	molecular	225:233	arg1	diffusion					235:243	(i) molecular diffusion	221:243	(i) molecular diffusion	221:243	Wet coagulated cellulose was impregnated with silica phase, polyethoxydisiloxane, using two methods: (i) molecular diffusion and (ii) forced flow induced by pressure difference.
25817671	5	31	theme	Nitrogen	524:531	arg1	analysis					544:551	Nitrogen adsorption analysis	524:551	Nitrogen adsorption analysis	524:551	Nitrogen adsorption analysis revealed an almost threefold increase in pores specific surface area, from cellulose aerogel alone to organic-inorganic composite.
25817671	7	32	theme	cellulose	938:946	arg1	pores					948:952	superinsulating mesoporous silica inside cellulose pores	897:952	superinsulating mesoporous silica inside cellulose pores	897:952	Thermal conductivity of composite aerogels was lower than that of cellulose aerogel due to the formation of superinsulating mesoporous silica inside cellulose pores.
25817671	7	33	theme	Thermal	789:795	arg1	conductivity					797:808	Thermal conductivity	789:808	Thermal conductivity of composite aerogels	789:830	Thermal conductivity of composite aerogels was lower than that of cellulose aerogel due to the formation of superinsulating mesoporous silica inside cellulose pores.
25817671	5	34	theme	adsorption	533:542	arg1	analysis					544:551	Nitrogen adsorption analysis	524:551	Nitrogen adsorption analysis	524:551	Nitrogen adsorption analysis revealed an almost threefold increase in pores specific surface area, from cellulose aerogel alone to organic-inorganic composite.
25817671	7	35	theme	pores	948:952	arg1	formation					884:892	the formation	880:892	the formation of superinsulating mesoporous silica inside cellulose pores	880:952	Thermal conductivity of composite aerogels was lower than that of cellulose aerogel due to the formation of superinsulating mesoporous silica inside cellulose pores.
25817671	6	36	theme	uniaxial	749:756	arg1	compression					758:768	uniaxial compression	749:768	uniaxial compression	749:768	Morphology, thermal conductivity and mechanical properties under uniaxial compression were investigated.
25817671	3	37	from	decrease	329:336	arg1	times					358:362	the impregnation times	341:362	the impregnation times	341:362	The latter allowed an enormous decrease in the impregnation times, by almost three orders of magnitude, for a sample with the same geometry.
25817671	2	38	theme	forced	254:259	arg1	methods					212:218	two methods	208:218	two methods: (i) molecular diffusion and (ii) forced flow induced by pressure difference	208:295	Wet coagulated cellulose was impregnated with silica phase, polyethoxydisiloxane, using two methods: (i) molecular diffusion and (ii) forced flow induced by pressure difference.
25817671	2	38	theme	forced	254:259	arg1	flow					261:264	(ii) forced flow	249:264	(ii) forced flow	249:264	Wet coagulated cellulose was impregnated with silica phase, polyethoxydisiloxane, using two methods: (i) molecular diffusion and (ii) forced flow induced by pressure difference.
25817671	7	39	theme	silica	924:929	arg1	pores					948:952	superinsulating mesoporous silica inside cellulose pores	897:952	superinsulating mesoporous silica inside cellulose pores	897:952	Thermal conductivity of composite aerogels was lower than that of cellulose aerogel due to the formation of superinsulating mesoporous silica inside cellulose pores.
25817671	1	40	theme	interpenetrated	45:59	arg1	networks					78:85	interpenetrated cellulose-silica networks	45:85	interpenetrated cellulose-silica networks	45:85	Aerogels based on interpenetrated cellulose-silica networks were prepared and characterised.
25817671	7	41	theme	inside	931:936	arg1	pores					948:952	superinsulating mesoporous silica inside cellulose pores	897:952	superinsulating mesoporous silica inside cellulose pores	897:952	Thermal conductivity of composite aerogels was lower than that of cellulose aerogel due to the formation of superinsulating mesoporous silica inside cellulose pores.
25817671	1	42	theme	cellulose-silica	61:76	arg1	networks					78:85	interpenetrated cellulose-silica networks	45:85	interpenetrated cellulose-silica networks	45:85	Aerogels based on interpenetrated cellulose-silica networks were prepared and characterised.
25817671	6	43	theme	mechanical	721:730	arg1	properties					732:741	mechanical properties	721:741	mechanical properties	721:741	Morphology, thermal conductivity and mechanical properties under uniaxial compression were investigated.
25817671	7	44	theme	superinsulating	897:911	arg1	pores					948:952	superinsulating mesoporous silica inside cellulose pores	897:952	superinsulating mesoporous silica inside cellulose pores	897:952	Thermal conductivity of composite aerogels was lower than that of cellulose aerogel due to the formation of superinsulating mesoporous silica inside cellulose pores.
25737227	6	0	from	changes	907:913	arg1	abundance					943:951	cell wall polysaccharide abundance	918:951	cell wall polysaccharide abundance	918:951	Syncytial development progressed faster in the resistant cultivar Chebec than in the susceptible cultivar Skiff, and was accompanied by changes in cell wall polysaccharide abundance, particularly (1,3;1,4)-β-glucan.
25737227	0	1	theme	-β-glucan	137:145	arg1	levels					147:152	(1,3;1,4)-β-glucan levels	128:152	(1,3;1,4)-β-glucan levels	128:152	The dynamics of cereal cyst nematode infection differ between susceptible and resistant barley cultivars and lead to changes in (1,3;1,4)-β-glucan levels and HvCslF gene transcript abundance.
25737227	1	2	theme	nematode	223:230	arg1	avenae					203:208	Heterodera avenae	192:208	Heterodera avenae (cereal cyst nematode, CCN)	192:236	Heterodera avenae (cereal cyst nematode, CCN) infects the roots of barley (Hordeum vulgare) forming syncytial feeding sites.
25737227	1	2	theme	nematode	223:230	arg1	CCN					233:235	cereal cyst nematode, CCN	211:235	CCN	233:235	Heterodera avenae (cereal cyst nematode, CCN) infects the roots of barley (Hordeum vulgare) forming syncytial feeding sites.
25737227	10	3	from	changes	1609:1615	arg1	genes					1643:1647	uncharacterized HvCslF genes	1620:1647	uncharacterized HvCslF genes	1620:1647	Dynamic transcriptional changes in uncharacterized HvCslF genes, possibly involved in (1,3;1,4)-β-glucan synthesis, suggest a role for these genes in the CCN infection process.
25737227	4	4	theme	transcriptional	619:633	arg1	analysis					635:642	histological, compositional and transcriptional analysis	587:642	histological, compositional and transcriptional analysis	587:642	Responses to CCN infection were investigated in resistant (Rha2) and susceptible barley cultivars through histological, compositional and transcriptional analysis.
25737227	0	5	theme	gene	165:168	arg1	abundance					181:189	HvCslF gene transcript abundance	158:189	HvCslF gene transcript abundance	158:189	The dynamics of cereal cyst nematode infection differ between susceptible and resistant barley cultivars and lead to changes in (1,3;1,4)-β-glucan levels and HvCslF gene transcript abundance.
25737227	4	6	theme	compositional	601:613	arg1	analysis					635:642	histological, compositional and transcriptional analysis	587:642	histological, compositional and transcriptional analysis	587:642	Responses to CCN infection were investigated in resistant (Rha2) and susceptible barley cultivars through histological, compositional and transcriptional analysis.
25737227	9	7	theme	CCN	1466:1468	arg1	development					1483:1493	CCN feeding site development	1466:1493	CCN feeding site development	1466:1493	During H. avenae infection, (1,3;1,4)-β-glucan may influence CCN feeding site development by limiting solute flow, similar to (1,3)-β-glucan during dicot cyst nematode infections.
25737227	4	8	theme	histological	587:598	arg1	analysis					635:642	histological, compositional and transcriptional analysis	587:642	histological, compositional and transcriptional analysis	587:642	Responses to CCN infection were investigated in resistant (Rha2) and susceptible barley cultivars through histological, compositional and transcriptional analysis.
25737227	10	9	theme	Dynamic	1585:1591	arg1	changes					1609:1615	Dynamic transcriptional changes	1585:1615	Dynamic transcriptional changes in uncharacterized HvCslF genes, possibly involved in (1,3;1,4)-β-glucan synthesis,	1585:1699	Dynamic transcriptional changes in uncharacterized HvCslF genes, possibly involved in (1,3;1,4)-β-glucan synthesis, suggest a role for these genes in the CCN infection process.
25737227	7	10	theme	SYNTHASE-LIKE	1080:1092	arg1	HvCslF10					1099:1106	HvCslF10	1099:1106	HvCslF10	1099:1106	Transcriptional profiling identified several glycosyl transferase genes, including CELLULOSE SYNTHASE-LIKE F10 (HvCslF10), which may contribute to differences in polysaccharide abundance between resistant and susceptible cultivars.
25737227	7	10	theme	SYNTHASE-LIKE	1080:1092	arg1	F10					1094:1096	CELLULOSE SYNTHASE-LIKE F10	1070:1096	CELLULOSE SYNTHASE-LIKE F10 (HvCslF10)	1070:1107	Transcriptional profiling identified several glycosyl transferase genes, including CELLULOSE SYNTHASE-LIKE F10 (HvCslF10), which may contribute to differences in polysaccharide abundance between resistant and susceptible cultivars.
25737227	10	11	theme	infection	1743:1751	arg1	process					1753:1759	the CCN infection process	1735:1759	the CCN infection process	1735:1759	Dynamic transcriptional changes in uncharacterized HvCslF genes, possibly involved in (1,3;1,4)-β-glucan synthesis, suggest a role for these genes in the CCN infection process.
25737227	9	12	theme	site	1478:1481	arg1	development					1483:1493	CCN feeding site development	1466:1493	CCN feeding site development	1466:1493	During H. avenae infection, (1,3;1,4)-β-glucan may influence CCN feeding site development by limiting solute flow, similar to (1,3)-β-glucan during dicot cyst nematode infections.
25737227	8	13	theme	sites	1301:1305	arg1	changes					1317:1323	specific changes	1308:1323	specific changes in cell wall-related transcript abundance	1308:1365	In barley, Rha2-mediated CCN resistance drives rapid deterioration of CCN feeding sites, specific changes in cell wall-related transcript abundance and changes in cell wall composition.
25737227	8	13	theme	sites	1301:1305	arg1	deterioration					1272:1284	rapid deterioration	1266:1284	rapid deterioration of CCN feeding sites	1266:1305	In barley, Rha2-mediated CCN resistance drives rapid deterioration of CCN feeding sites, specific changes in cell wall-related transcript abundance and changes in cell wall composition.
25737227	8	13	theme	sites	1301:1305	arg1	changes					1371:1377	changes	1371:1377	changes in cell wall composition	1371:1402	In barley, Rha2-mediated CCN resistance drives rapid deterioration of CCN feeding sites, specific changes in cell wall-related transcript abundance and changes in cell wall composition.
25737227	8	14	theme	CCN	1244:1246	arg1	resistance					1248:1257	Rha2-mediated CCN resistance	1230:1257	Rha2-mediated CCN resistance	1230:1257	In barley, Rha2-mediated CCN resistance drives rapid deterioration of CCN feeding sites, specific changes in cell wall-related transcript abundance and changes in cell wall composition.
25737227	6	15	theme	polysaccharide	928:941	arg1	abundance					943:951	cell wall polysaccharide abundance	918:951	cell wall polysaccharide abundance	918:951	Syncytial development progressed faster in the resistant cultivar Chebec than in the susceptible cultivar Skiff, and was accompanied by changes in cell wall polysaccharide abundance, particularly (1,3;1,4)-β-glucan.
25737227	8	16	theme	specific	1308:1315	arg1	changes					1317:1323	specific changes	1308:1323	specific changes in cell wall-related transcript abundance	1308:1365	In barley, Rha2-mediated CCN resistance drives rapid deterioration of CCN feeding sites, specific changes in cell wall-related transcript abundance and changes in cell wall composition.
25737227	8	16	theme	specific	1308:1315	arg1	deterioration					1272:1284	rapid deterioration	1266:1284	rapid deterioration of CCN feeding sites	1266:1305	In barley, Rha2-mediated CCN resistance drives rapid deterioration of CCN feeding sites, specific changes in cell wall-related transcript abundance and changes in cell wall composition.
25737227	10	17	theme	uncharacterized	1620:1634	arg1	genes					1643:1647	uncharacterized HvCslF genes	1620:1647	uncharacterized HvCslF genes	1620:1647	Dynamic transcriptional changes in uncharacterized HvCslF genes, possibly involved in (1,3;1,4)-β-glucan synthesis, suggest a role for these genes in the CCN infection process.
25737227	9	18	theme	solute	1507:1512	arg1	flow					1514:1517	solute flow	1507:1517	solute flow	1507:1517	During H. avenae infection, (1,3;1,4)-β-glucan may influence CCN feeding site development by limiting solute flow, similar to (1,3)-β-glucan during dicot cyst nematode infections.
25737227	6	19	theme	cell	918:921	arg1	abundance					943:951	cell wall polysaccharide abundance	918:951	cell wall polysaccharide abundance	918:951	Syncytial development progressed faster in the resistant cultivar Chebec than in the susceptible cultivar Skiff, and was accompanied by changes in cell wall polysaccharide abundance, particularly (1,3;1,4)-β-glucan.
25737227	7	20	theme	glycosyl	1032:1039	arg1	F10					1094:1096	CELLULOSE SYNTHASE-LIKE F10	1070:1096	CELLULOSE SYNTHASE-LIKE F10 (HvCslF10)	1070:1107	Transcriptional profiling identified several glycosyl transferase genes, including CELLULOSE SYNTHASE-LIKE F10 (HvCslF10), which may contribute to differences in polysaccharide abundance between resistant and susceptible cultivars.
25737227	7	20	theme	glycosyl	1032:1039	arg1	genes					1053:1057	several glycosyl transferase genes	1024:1057	several glycosyl transferase genes	1024:1057	Transcriptional profiling identified several glycosyl transferase genes, including CELLULOSE SYNTHASE-LIKE F10 (HvCslF10), which may contribute to differences in polysaccharide abundance between resistant and susceptible cultivars.
25737227	0	21	dep	1,3	129:131	arg1	1,4					133:135	1,4	133:135	1,3;1,4	129:135	The dynamics of cereal cyst nematode infection differ between susceptible and resistant barley cultivars and lead to changes in (1,3;1,4)-β-glucan levels and HvCslF gene transcript abundance.
25737227	8	22	theme	wall-related	1333:1344	arg1	abundance					1357:1365	cell wall-related transcript abundance	1328:1365	cell wall-related transcript abundance	1328:1365	In barley, Rha2-mediated CCN resistance drives rapid deterioration of CCN feeding sites, specific changes in cell wall-related transcript abundance and changes in cell wall composition.
25737227	7	23	from	differences	1134:1144	arg1	abundance					1164:1172	polysaccharide abundance	1149:1172	polysaccharide abundance	1149:1172	Transcriptional profiling identified several glycosyl transferase genes, including CELLULOSE SYNTHASE-LIKE F10 (HvCslF10), which may contribute to differences in polysaccharide abundance between resistant and susceptible cultivars.
25737227	5	24	theme	cyst	754:757	arg1	maturation					759:768	subsequent cyst maturation	743:768	subsequent cyst maturation	743:768	Two phases were identified that influence CCN viability, including feeding site establishment and subsequent cyst maturation.
25737227	9	25	dep	1,3	1434:1436	arg1	1,4					1438:1440	1,4	1438:1440	1,4	1438:1440	During H. avenae infection, (1,3;1,4)-β-glucan may influence CCN feeding site development by limiting solute flow, similar to (1,3)-β-glucan during dicot cyst nematode infections.
25737227	0	26	theme	barley	88:93	arg1	cultivars					95:103	susceptible and resistant barley cultivars	62:103	susceptible and resistant barley cultivars	62:103	The dynamics of cereal cyst nematode infection differ between susceptible and resistant barley cultivars and lead to changes in (1,3;1,4)-β-glucan levels and HvCslF gene transcript abundance.
25737227	1	27	theme	feeding	302:308	arg1	sites					310:314	syncytial feeding sites	292:314	syncytial feeding sites	292:314	Heterodera avenae (cereal cyst nematode, CCN) infects the roots of barley (Hordeum vulgare) forming syncytial feeding sites.
25737227	8	28	theme	cell	1382:1385	arg1	composition					1392:1402	cell wall composition	1382:1402	cell wall composition	1382:1402	In barley, Rha2-mediated CCN resistance drives rapid deterioration of CCN feeding sites, specific changes in cell wall-related transcript abundance and changes in cell wall composition.
25737227	5	29	theme	site	720:723	arg1	establishment					725:737	feeding site establishment	712:737	feeding site establishment	712:737	Two phases were identified that influence CCN viability, including feeding site establishment and subsequent cyst maturation.
25737227	9	30	theme	dicot	1553:1557	arg1	infections					1573:1582	dicot cyst nematode infections	1553:1582	dicot cyst nematode infections	1553:1582	During H. avenae infection, (1,3;1,4)-β-glucan may influence CCN feeding site development by limiting solute flow, similar to (1,3)-β-glucan during dicot cyst nematode infections.
25737227	10	31	dep	synthesis	1690:1698	arg1	1,3					1672:1674	1,3	1672:1674	1,3;1,4	1672:1678	Dynamic transcriptional changes in uncharacterized HvCslF genes, possibly involved in (1,3;1,4)-β-glucan synthesis, suggest a role for these genes in the CCN infection process.
25737227	2	32	theme	host	330:333	arg1	plants					335:340	resistant host plants	320:340	resistant host plants	320:340	In resistant host plants, relatively few females develop to maturity.
25737227	9	33	theme	nematode	1564:1571	arg1	infections					1573:1582	dicot cyst nematode infections	1553:1582	dicot cyst nematode infections	1553:1582	During H. avenae infection, (1,3;1,4)-β-glucan may influence CCN feeding site development by limiting solute flow, similar to (1,3)-β-glucan during dicot cyst nematode infections.
25737227	6	34	theme	cultivar	868:875	arg1	Skiff					877:881	the susceptible cultivar Skiff	852:881	the susceptible cultivar Skiff	852:881	Syncytial development progressed faster in the resistant cultivar Chebec than in the susceptible cultivar Skiff, and was accompanied by changes in cell wall polysaccharide abundance, particularly (1,3;1,4)-β-glucan.
25737227	2	35	theme	few	354:356	arg1	females					358:364	relatively few females	343:364	relatively few females	343:364	In resistant host plants, relatively few females develop to maturity.
25737227	7	36	theme	polysaccharide	1149:1162	arg1	abundance					1164:1172	polysaccharide abundance	1149:1172	polysaccharide abundance	1149:1172	Transcriptional profiling identified several glycosyl transferase genes, including CELLULOSE SYNTHASE-LIKE F10 (HvCslF10), which may contribute to differences in polysaccharide abundance between resistant and susceptible cultivars.
25737227	0	37	theme	cyst	23:26	arg1	infection					37:45	cereal cyst nematode infection	16:45	cereal cyst nematode infection	16:45	The dynamics of cereal cyst nematode infection differ between susceptible and resistant barley cultivars and lead to changes in (1,3;1,4)-β-glucan levels and HvCslF gene transcript abundance.
25737227	0	38	from	abundance	181:189	arg1	levels					147:152	(1,3;1,4)-β-glucan levels	128:152	(1,3;1,4)-β-glucan levels	128:152	The dynamics of cereal cyst nematode infection differ between susceptible and resistant barley cultivars and lead to changes in (1,3;1,4)-β-glucan levels and HvCslF gene transcript abundance.
25737227	9	39	dep	-β-glucan	1442:1450	arg1	1,3					1434:1436	1,3	1434:1436	1,3;1,4	1434:1440	During H. avenae infection, (1,3;1,4)-β-glucan may influence CCN feeding site development by limiting solute flow, similar to (1,3)-β-glucan during dicot cyst nematode infections.
25737227	3	40	theme	biochemical	431:441	arg1	changes					443:449	the physiological and biochemical changes	409:449	the physiological and biochemical changes induced during CCN infection	409:478	Little is known about the physiological and biochemical changes induced during CCN infection.
25737227	0	41	theme	infection	37:45	arg1	dynamics					4:11	The dynamics	0:11	The dynamics of cereal cyst nematode infection	0:45	The dynamics of cereal cyst nematode infection differ between susceptible and resistant barley cultivars and lead to changes in (1,3;1,4)-β-glucan levels and HvCslF gene transcript abundance.
25737227	3	42	theme	physiological	413:425	arg1	changes					443:449	the physiological and biochemical changes	409:449	the physiological and biochemical changes induced during CCN infection	409:478	Little is known about the physiological and biochemical changes induced during CCN infection.
25737227	9	43	theme	H. avenae	1412:1420	arg1	infection					1422:1430	H. avenae infection	1412:1430	H. avenae infection	1412:1430	During H. avenae infection, (1,3;1,4)-β-glucan may influence CCN feeding site development by limiting solute flow, similar to (1,3)-β-glucan during dicot cyst nematode infections.
25737227	6	44	theme	cultivar	828:835	arg1	Chebec					837:842	the resistant cultivar Chebec	814:842	the resistant cultivar Chebec	814:842	Syncytial development progressed faster in the resistant cultivar Chebec than in the susceptible cultivar Skiff, and was accompanied by changes in cell wall polysaccharide abundance, particularly (1,3;1,4)-β-glucan.
25737227	3	45	theme	CCN	466:468	arg1	infection					470:478	CCN infection	466:478	CCN infection	466:478	Little is known about the physiological and biochemical changes induced during CCN infection.
25737227	4	46	theme	barley	562:567	arg1	cultivars					569:577	resistant (Rha2) and susceptible barley cultivars	529:577	resistant (Rha2) and susceptible barley cultivars	529:577	Responses to CCN infection were investigated in resistant (Rha2) and susceptible barley cultivars through histological, compositional and transcriptional analysis.
25737227	5	47	theme	subsequent	743:752	arg1	maturation					759:768	subsequent cyst maturation	743:768	subsequent cyst maturation	743:768	Two phases were identified that influence CCN viability, including feeding site establishment and subsequent cyst maturation.
25737227	1	48	theme	cyst	218:221	arg1	avenae					203:208	Heterodera avenae	192:208	Heterodera avenae (cereal cyst nematode, CCN)	192:236	Heterodera avenae (cereal cyst nematode, CCN) infects the roots of barley (Hordeum vulgare) forming syncytial feeding sites.
25737227	1	48	theme	cyst	218:221	arg1	CCN					233:235	cereal cyst nematode, CCN	211:235	CCN	233:235	Heterodera avenae (cereal cyst nematode, CCN) infects the roots of barley (Hordeum vulgare) forming syncytial feeding sites.
25737227	4	49	theme	resistant	529:537	arg1	cultivars					569:577	resistant (Rha2) and susceptible barley cultivars	529:577	resistant (Rha2) and susceptible barley cultivars	529:577	Responses to CCN infection were investigated in resistant (Rha2) and susceptible barley cultivars through histological, compositional and transcriptional analysis.
25737227	0	50	theme	HvCslF	158:163	arg1	abundance					181:189	HvCslF gene transcript abundance	158:189	HvCslF gene transcript abundance	158:189	The dynamics of cereal cyst nematode infection differ between susceptible and resistant barley cultivars and lead to changes in (1,3;1,4)-β-glucan levels and HvCslF gene transcript abundance.
25737227	10	51	theme	CCN	1739:1741	arg1	process					1753:1759	the CCN infection process	1735:1759	the CCN infection process	1735:1759	Dynamic transcriptional changes in uncharacterized HvCslF genes, possibly involved in (1,3;1,4)-β-glucan synthesis, suggest a role for these genes in the CCN infection process.
25737227	0	52	theme	transcript	170:179	arg1	abundance					181:189	HvCslF gene transcript abundance	158:189	HvCslF gene transcript abundance	158:189	The dynamics of cereal cyst nematode infection differ between susceptible and resistant barley cultivars and lead to changes in (1,3;1,4)-β-glucan levels and HvCslF gene transcript abundance.
25737227	10	53	from	role	1711:1714	arg1	process					1753:1759	the CCN infection process	1735:1759	the CCN infection process	1735:1759	Dynamic transcriptional changes in uncharacterized HvCslF genes, possibly involved in (1,3;1,4)-β-glucan synthesis, suggest a role for these genes in the CCN infection process.
25737227	6	54	theme	Syncytial	771:779	arg1	development					781:791	Syncytial development	771:791	Syncytial development	771:791	Syncytial development progressed faster in the resistant cultivar Chebec than in the susceptible cultivar Skiff, and was accompanied by changes in cell wall polysaccharide abundance, particularly (1,3;1,4)-β-glucan.
25737227	8	55	theme	rapid	1266:1270	arg1	changes					1317:1323	specific changes	1308:1323	specific changes in cell wall-related transcript abundance	1308:1365	In barley, Rha2-mediated CCN resistance drives rapid deterioration of CCN feeding sites, specific changes in cell wall-related transcript abundance and changes in cell wall composition.
25737227	8	55	theme	rapid	1266:1270	arg1	deterioration					1272:1284	rapid deterioration	1266:1284	rapid deterioration of CCN feeding sites	1266:1305	In barley, Rha2-mediated CCN resistance drives rapid deterioration of CCN feeding sites, specific changes in cell wall-related transcript abundance and changes in cell wall composition.
25737227	8	55	theme	rapid	1266:1270	arg1	changes					1371:1377	changes	1371:1377	changes in cell wall composition	1371:1402	In barley, Rha2-mediated CCN resistance drives rapid deterioration of CCN feeding sites, specific changes in cell wall-related transcript abundance and changes in cell wall composition.
25737227	10	56	theme	transcriptional	1593:1607	arg1	changes					1609:1615	Dynamic transcriptional changes	1585:1615	Dynamic transcriptional changes in uncharacterized HvCslF genes, possibly involved in (1,3;1,4)-β-glucan synthesis,	1585:1699	Dynamic transcriptional changes in uncharacterized HvCslF genes, possibly involved in (1,3;1,4)-β-glucan synthesis, suggest a role for these genes in the CCN infection process.
25737227	1	57	theme	Heterodera	192:201	arg1	avenae					203:208	Heterodera avenae	192:208	Heterodera avenae (cereal cyst nematode, CCN)	192:236	Heterodera avenae (cereal cyst nematode, CCN) infects the roots of barley (Hordeum vulgare) forming syncytial feeding sites.
25737227	1	57	theme	Heterodera	192:201	arg1	CCN					233:235	cereal cyst nematode, CCN	211:235	CCN	233:235	Heterodera avenae (cereal cyst nematode, CCN) infects the roots of barley (Hordeum vulgare) forming syncytial feeding sites.
25737227	7	58	theme	CELLULOSE	1070:1078	arg1	HvCslF10					1099:1106	HvCslF10	1099:1106	HvCslF10	1099:1106	Transcriptional profiling identified several glycosyl transferase genes, including CELLULOSE SYNTHASE-LIKE F10 (HvCslF10), which may contribute to differences in polysaccharide abundance between resistant and susceptible cultivars.
25737227	7	58	theme	CELLULOSE	1070:1078	arg1	F10					1094:1096	CELLULOSE SYNTHASE-LIKE F10	1070:1096	CELLULOSE SYNTHASE-LIKE F10 (HvCslF10)	1070:1107	Transcriptional profiling identified several glycosyl transferase genes, including CELLULOSE SYNTHASE-LIKE F10 (HvCslF10), which may contribute to differences in polysaccharide abundance between resistant and susceptible cultivars.
25737227	9	59	theme	feeding	1470:1476	arg1	development					1483:1493	CCN feeding site development	1466:1493	CCN feeding site development	1466:1493	During H. avenae infection, (1,3;1,4)-β-glucan may influence CCN feeding site development by limiting solute flow, similar to (1,3)-β-glucan during dicot cyst nematode infections.
25737227	5	60	theme	CCN	687:689	arg1	viability					691:699	CCN viability	687:699	CCN viability	687:699	Two phases were identified that influence CCN viability, including feeding site establishment and subsequent cyst maturation.
25737227	0	61	dep	levels	147:152	arg1	1,3					129:131	1,3	129:131	1,3;1,4	129:135	The dynamics of cereal cyst nematode infection differ between susceptible and resistant barley cultivars and lead to changes in (1,3;1,4)-β-glucan levels and HvCslF gene transcript abundance.
25737227	8	62	theme	feeding	1293:1299	arg1	sites					1301:1305	CCN feeding sites	1289:1305	CCN feeding sites	1289:1305	In barley, Rha2-mediated CCN resistance drives rapid deterioration of CCN feeding sites, specific changes in cell wall-related transcript abundance and changes in cell wall composition.
25737227	10	63	theme	HvCslF	1636:1641	arg1	genes					1643:1647	uncharacterized HvCslF genes	1620:1647	uncharacterized HvCslF genes	1620:1647	Dynamic transcriptional changes in uncharacterized HvCslF genes, possibly involved in (1,3;1,4)-β-glucan synthesis, suggest a role for these genes in the CCN infection process.
25737227	7	64	theme	transferase	1041:1051	arg1	F10					1094:1096	CELLULOSE SYNTHASE-LIKE F10	1070:1096	CELLULOSE SYNTHASE-LIKE F10 (HvCslF10)	1070:1107	Transcriptional profiling identified several glycosyl transferase genes, including CELLULOSE SYNTHASE-LIKE F10 (HvCslF10), which may contribute to differences in polysaccharide abundance between resistant and susceptible cultivars.
25737227	7	64	theme	transferase	1041:1051	arg1	genes					1053:1057	several glycosyl transferase genes	1024:1057	several glycosyl transferase genes	1024:1057	Transcriptional profiling identified several glycosyl transferase genes, including CELLULOSE SYNTHASE-LIKE F10 (HvCslF10), which may contribute to differences in polysaccharide abundance between resistant and susceptible cultivars.
25737227	4	65	dep	resistant	529:537	arg1	Rha2					540:543	Rha2	540:543	Rha2	540:543	Responses to CCN infection were investigated in resistant (Rha2) and susceptible barley cultivars through histological, compositional and transcriptional analysis.
25737227	8	66	theme	Rha2-mediated	1230:1242	arg1	resistance					1248:1257	Rha2-mediated CCN resistance	1230:1257	Rha2-mediated CCN resistance	1230:1257	In barley, Rha2-mediated CCN resistance drives rapid deterioration of CCN feeding sites, specific changes in cell wall-related transcript abundance and changes in cell wall composition.
25737227	6	67	theme	wall	923:926	arg1	abundance					943:951	cell wall polysaccharide abundance	918:951	cell wall polysaccharide abundance	918:951	Syncytial development progressed faster in the resistant cultivar Chebec than in the susceptible cultivar Skiff, and was accompanied by changes in cell wall polysaccharide abundance, particularly (1,3;1,4)-β-glucan.
25737227	7	68	theme	several	1024:1030	arg1	F10					1094:1096	CELLULOSE SYNTHASE-LIKE F10	1070:1096	CELLULOSE SYNTHASE-LIKE F10 (HvCslF10)	1070:1107	Transcriptional profiling identified several glycosyl transferase genes, including CELLULOSE SYNTHASE-LIKE F10 (HvCslF10), which may contribute to differences in polysaccharide abundance between resistant and susceptible cultivars.
25737227	7	68	theme	several	1024:1030	arg1	genes					1053:1057	several glycosyl transferase genes	1024:1057	several glycosyl transferase genes	1024:1057	Transcriptional profiling identified several glycosyl transferase genes, including CELLULOSE SYNTHASE-LIKE F10 (HvCslF10), which may contribute to differences in polysaccharide abundance between resistant and susceptible cultivars.
25737227	7	69	theme	Transcriptional	987:1001	arg1	profiling					1003:1011	Transcriptional profiling	987:1011	Transcriptional profiling	987:1011	Transcriptional profiling identified several glycosyl transferase genes, including CELLULOSE SYNTHASE-LIKE F10 (HvCslF10), which may contribute to differences in polysaccharide abundance between resistant and susceptible cultivars.
25737227	6	70	dep	1,3	968:970	arg1	1,4					972:974	1,4	972:974	1,4	972:974	Syncytial development progressed faster in the resistant cultivar Chebec than in the susceptible cultivar Skiff, and was accompanied by changes in cell wall polysaccharide abundance, particularly (1,3;1,4)-β-glucan.
25737227	9	71	dep	-β-glucan	1536:1544	arg1	1,3					1532:1534	1,3	1532:1534	1,3	1532:1534	During H. avenae infection, (1,3;1,4)-β-glucan may influence CCN feeding site development by limiting solute flow, similar to (1,3)-β-glucan during dicot cyst nematode infections.
25737227	0	72	theme	susceptible	62:72	arg1	cultivars					95:103	susceptible and resistant barley cultivars	62:103	susceptible and resistant barley cultivars	62:103	The dynamics of cereal cyst nematode infection differ between susceptible and resistant barley cultivars and lead to changes in (1,3;1,4)-β-glucan levels and HvCslF gene transcript abundance.
25737227	8	73	theme	CCN	1289:1291	arg1	sites					1301:1305	CCN feeding sites	1289:1305	CCN feeding sites	1289:1305	In barley, Rha2-mediated CCN resistance drives rapid deterioration of CCN feeding sites, specific changes in cell wall-related transcript abundance and changes in cell wall composition.
25737227	9	74	theme	similar	1520:1526	arg1	flow					1514:1517	solute flow	1507:1517	solute flow	1507:1517	During H. avenae infection, (1,3;1,4)-β-glucan may influence CCN feeding site development by limiting solute flow, similar to (1,3)-β-glucan during dicot cyst nematode infections.
25737227	8	75	theme	cell	1328:1331	arg1	abundance					1357:1365	cell wall-related transcript abundance	1328:1365	cell wall-related transcript abundance	1328:1365	In barley, Rha2-mediated CCN resistance drives rapid deterioration of CCN feeding sites, specific changes in cell wall-related transcript abundance and changes in cell wall composition.
25737227	0	76	theme	resistant	78:86	arg1	cultivars					95:103	susceptible and resistant barley cultivars	62:103	susceptible and resistant barley cultivars	62:103	The dynamics of cereal cyst nematode infection differ between susceptible and resistant barley cultivars and lead to changes in (1,3;1,4)-β-glucan levels and HvCslF gene transcript abundance.
25737227	6	77	dep	-β-glucan	976:984	arg1	1,3					968:970	1,3	968:970	1,3;1,4	968:974	Syncytial development progressed faster in the resistant cultivar Chebec than in the susceptible cultivar Skiff, and was accompanied by changes in cell wall polysaccharide abundance, particularly (1,3;1,4)-β-glucan.
25737227	8	78	theme	transcript	1346:1355	arg1	abundance					1357:1365	cell wall-related transcript abundance	1328:1365	cell wall-related transcript abundance	1328:1365	In barley, Rha2-mediated CCN resistance drives rapid deterioration of CCN feeding sites, specific changes in cell wall-related transcript abundance and changes in cell wall composition.
25737227	1	79	theme	syncytial	292:300	arg1	sites					310:314	syncytial feeding sites	292:314	syncytial feeding sites	292:314	Heterodera avenae (cereal cyst nematode, CCN) infects the roots of barley (Hordeum vulgare) forming syncytial feeding sites.
25737227	8	80	from	changes	1317:1323	arg1	composition					1392:1402	cell wall composition	1382:1402	cell wall composition	1382:1402	In barley, Rha2-mediated CCN resistance drives rapid deterioration of CCN feeding sites, specific changes in cell wall-related transcript abundance and changes in cell wall composition.
25737227	8	80	from	changes	1317:1323	arg1	abundance					1357:1365	cell wall-related transcript abundance	1328:1365	cell wall-related transcript abundance	1328:1365	In barley, Rha2-mediated CCN resistance drives rapid deterioration of CCN feeding sites, specific changes in cell wall-related transcript abundance and changes in cell wall composition.
25737227	5	81	theme	feeding	712:718	arg1	establishment					725:737	feeding site establishment	712:737	feeding site establishment	712:737	Two phases were identified that influence CCN viability, including feeding site establishment and subsequent cyst maturation.
25737227	7	82	theme	susceptible	1196:1206	arg1	cultivars					1208:1216	resistant and susceptible cultivars	1182:1216	resistant and susceptible cultivars	1182:1216	Transcriptional profiling identified several glycosyl transferase genes, including CELLULOSE SYNTHASE-LIKE F10 (HvCslF10), which may contribute to differences in polysaccharide abundance between resistant and susceptible cultivars.
25737227	7	83	theme	resistant	1182:1190	arg1	cultivars					1208:1216	resistant and susceptible cultivars	1182:1216	resistant and susceptible cultivars	1182:1216	Transcriptional profiling identified several glycosyl transferase genes, including CELLULOSE SYNTHASE-LIKE F10 (HvCslF10), which may contribute to differences in polysaccharide abundance between resistant and susceptible cultivars.
25737227	9	84	theme	cyst	1559:1562	arg1	infections					1573:1582	dicot cyst nematode infections	1553:1582	dicot cyst nematode infections	1553:1582	During H. avenae infection, (1,3;1,4)-β-glucan may influence CCN feeding site development by limiting solute flow, similar to (1,3)-β-glucan during dicot cyst nematode infections.
25737227	8	85	theme	wall	1387:1390	arg1	composition					1392:1402	cell wall composition	1382:1402	cell wall composition	1382:1402	In barley, Rha2-mediated CCN resistance drives rapid deterioration of CCN feeding sites, specific changes in cell wall-related transcript abundance and changes in cell wall composition.
25737227	2	86	theme	resistant	320:328	arg1	plants					335:340	resistant host plants	320:340	resistant host plants	320:340	In resistant host plants, relatively few females develop to maturity.
25737227	0	87	theme	cereal	16:21	arg1	infection					37:45	cereal cyst nematode infection	16:45	cereal cyst nematode infection	16:45	The dynamics of cereal cyst nematode infection differ between susceptible and resistant barley cultivars and lead to changes in (1,3;1,4)-β-glucan levels and HvCslF gene transcript abundance.
25737227	0	88	theme	nematode	28:35	arg1	infection					37:45	cereal cyst nematode infection	16:45	cereal cyst nematode infection	16:45	The dynamics of cereal cyst nematode infection differ between susceptible and resistant barley cultivars and lead to changes in (1,3;1,4)-β-glucan levels and HvCslF gene transcript abundance.
25737227	6	89	theme	susceptible	856:866	arg1	Skiff					877:881	the susceptible cultivar Skiff	852:881	the susceptible cultivar Skiff	852:881	Syncytial development progressed faster in the resistant cultivar Chebec than in the susceptible cultivar Skiff, and was accompanied by changes in cell wall polysaccharide abundance, particularly (1,3;1,4)-β-glucan.
25737227	10	90	dep	1,3	1672:1674	arg1	1,4					1676:1678	1,4	1676:1678	1,4	1676:1678	Dynamic transcriptional changes in uncharacterized HvCslF genes, possibly involved in (1,3;1,4)-β-glucan synthesis, suggest a role for these genes in the CCN infection process.
25737227	1	91	theme	barley	259:264	arg1	roots					250:254	the roots	246:254	the roots of barley (Hordeum vulgare) forming syncytial feeding sites	246:314	Heterodera avenae (cereal cyst nematode, CCN) infects the roots of barley (Hordeum vulgare) forming syncytial feeding sites.
25737227	4	92	theme	CCN	494:496	arg1	infection					498:506	CCN infection	494:506	CCN infection	494:506	Responses to CCN infection were investigated in resistant (Rha2) and susceptible barley cultivars through histological, compositional and transcriptional analysis.
25737227	1	93	theme	Hordeum	267:273	arg1	barley					259:264	barley	259:264	barley (Hordeum vulgare) forming syncytial feeding sites	259:314	Heterodera avenae (cereal cyst nematode, CCN) infects the roots of barley (Hordeum vulgare) forming syncytial feeding sites.
25737227	1	93	theme	Hordeum	267:273	arg1	vulgare					275:281	Hordeum vulgare	267:281	Hordeum vulgare	267:281	Heterodera avenae (cereal cyst nematode, CCN) infects the roots of barley (Hordeum vulgare) forming syncytial feeding sites.
25737227	4	94	theme	susceptible	550:560	arg1	cultivars					569:577	resistant (Rha2) and susceptible barley cultivars	529:577	resistant (Rha2) and susceptible barley cultivars	529:577	Responses to CCN infection were investigated in resistant (Rha2) and susceptible barley cultivars through histological, compositional and transcriptional analysis.
25737227	10	95	theme	-β-glucan	1680:1688	arg1	synthesis					1690:1698	(1,3;1,4)-β-glucan synthesis	1671:1698	(1,3;1,4)-β-glucan synthesis	1671:1698	Dynamic transcriptional changes in uncharacterized HvCslF genes, possibly involved in (1,3;1,4)-β-glucan synthesis, suggest a role for these genes in the CCN infection process.
25737227	8	96	from	changes	1371:1377	arg1	composition					1392:1402	cell wall composition	1382:1402	cell wall composition	1382:1402	In barley, Rha2-mediated CCN resistance drives rapid deterioration of CCN feeding sites, specific changes in cell wall-related transcript abundance and changes in cell wall composition.
25737227	8	96	from	changes	1371:1377	arg1	abundance					1357:1365	cell wall-related transcript abundance	1328:1365	cell wall-related transcript abundance	1328:1365	In barley, Rha2-mediated CCN resistance drives rapid deterioration of CCN feeding sites, specific changes in cell wall-related transcript abundance and changes in cell wall composition.
25737227	6	97	theme	resistant	818:826	arg1	Chebec					837:842	the resistant cultivar Chebec	814:842	the resistant cultivar Chebec	814:842	Syncytial development progressed faster in the resistant cultivar Chebec than in the susceptible cultivar Skiff, and was accompanied by changes in cell wall polysaccharide abundance, particularly (1,3;1,4)-β-glucan.
25737227	0	98	from	changes	117:123	arg1	levels					147:152	(1,3;1,4)-β-glucan levels	128:152	(1,3;1,4)-β-glucan levels	128:152	The dynamics of cereal cyst nematode infection differ between susceptible and resistant barley cultivars and lead to changes in (1,3;1,4)-β-glucan levels and HvCslF gene transcript abundance.
25737227	1	99	theme	cereal	211:216	arg1	avenae					203:208	Heterodera avenae	192:208	Heterodera avenae (cereal cyst nematode, CCN)	192:236	Heterodera avenae (cereal cyst nematode, CCN) infects the roots of barley (Hordeum vulgare) forming syncytial feeding sites.
25737227	1	99	theme	cereal	211:216	arg1	CCN					233:235	cereal cyst nematode, CCN	211:235	CCN	233:235	Heterodera avenae (cereal cyst nematode, CCN) infects the roots of barley (Hordeum vulgare) forming syncytial feeding sites.
25878501	8	0	theme	fungicidal	1068:1077	arg1	agents					1079:1084	fungicidal agents	1068:1084	fungicidal agents in the future	1068:1098	The fabricated spheres had macroporous property, and could be used for many applications such as fungicidal agents in the future.
25878501	5	1	theme	1.7	595:597	arg1	mm					599:600	mm	599:600	mm	599:600	The diameter of the synthesized chitosan composite particles ranged from 1.7 mm to 2.5 mm, and the embedded silver nanoparticles were measured to be 15 ± 3.3 nm.
25878501	6	2	theme	diffraction	785:795	arg1	analyses					697:704	the analyses	693:704	the analyses of ultraviolet-visible spectroscopy, energy dispersive spectroscopy, and X-ray diffraction	693:795	Further, the analyses of ultraviolet-visible spectroscopy, energy dispersive spectroscopy, and X-ray diffraction were employed to characterize the prepared composites.
25878501	3	3	theme	silver	340:345	arg1	particles					379:387	silver nanoparticles-embedded chitosan particles	340:387	silver nanoparticles-embedded chitosan particles	340:387	The present article describes a novel approach for one-step synthesis of silver nanoparticles-embedded chitosan particles.
25878501	5	4	theme	silver	630:635	arg1	nanoparticles					637:649	the embedded silver nanoparticles	617:649	the embedded silver nanoparticles	617:649	The diameter of the synthesized chitosan composite particles ranged from 1.7 mm to 2.5 mm, and the embedded silver nanoparticles were measured to be 15 ± 3.3 nm.
25878501	5	4	theme	silver	630:635	arg1	nm					680:681	15 ± 3.3 nm	671:681	15 ± 3.3 nm	671:681	The diameter of the synthesized chitosan composite particles ranged from 1.7 mm to 2.5 mm, and the embedded silver nanoparticles were measured to be 15 ± 3.3 nm.
25878501	8	5	theme	many	1042:1045	arg1	agents					1079:1084	fungicidal agents	1068:1084	fungicidal agents in the future	1068:1098	The fabricated spheres had macroporous property, and could be used for many applications such as fungicidal agents in the future.
25878501	8	5	theme	many	1042:1045	arg1	applications					1047:1058	many applications	1042:1058	many applications such as fungicidal agents in the future	1042:1098	The fabricated spheres had macroporous property, and could be used for many applications such as fungicidal agents in the future.
25878501	5	6	dep	mm	599:600	arg1	to					602:603	to	602:603	to	602:603	The diameter of the synthesized chitosan composite particles ranged from 1.7 mm to 2.5 mm, and the embedded silver nanoparticles were measured to be 15 ± 3.3 nm.
25878501	6	7	theme	dispersive	750:759	arg1	spectroscopy					761:772	energy dispersive spectroscopy	743:772	energy dispersive spectroscopy	743:772	Further, the analyses of ultraviolet-visible spectroscopy, energy dispersive spectroscopy, and X-ray diffraction were employed to characterize the prepared composites.
25878501	8	8	theme	macroporous	998:1008	arg1	property					1010:1017	macroporous property	998:1017	macroporous property	998:1017	The fabricated spheres had macroporous property, and could be used for many applications such as fungicidal agents in the future.
25878501	6	9	theme	spectroscopy	729:740	arg1	analyses					697:704	the analyses	693:704	the analyses of ultraviolet-visible spectroscopy, energy dispersive spectroscopy, and X-ray diffraction	693:795	Further, the analyses of ultraviolet-visible spectroscopy, energy dispersive spectroscopy, and X-ray diffraction were employed to characterize the prepared composites.
25878501	6	10	theme	energy	743:748	arg1	spectroscopy					761:772	energy dispersive spectroscopy	743:772	energy dispersive spectroscopy	743:772	Further, the analyses of ultraviolet-visible spectroscopy, energy dispersive spectroscopy, and X-ray diffraction were employed to characterize the prepared composites.
25878501	6	11	theme	ultraviolet-visible	709:727	arg1	spectroscopy					729:740	ultraviolet-visible spectroscopy	709:740	ultraviolet-visible spectroscopy	709:740	Further, the analyses of ultraviolet-visible spectroscopy, energy dispersive spectroscopy, and X-ray diffraction were employed to characterize the prepared composites.
25878501	3	12	theme	particles	379:387	arg1	synthesis					327:335	one-step synthesis	318:335	one-step synthesis of silver nanoparticles-embedded chitosan particles	318:387	The present article describes a novel approach for one-step synthesis of silver nanoparticles-embedded chitosan particles.
25878501	1	13	theme	Silver	87:92	arg1	nanoparticles					94:106	Silver nanoparticles	87:106	Silver nanoparticles	87:106	Silver nanoparticles have been used in various fields, and several synthesis processes have been developed.
25878501	5	14	theme	synthesized	542:552	arg1	particles					573:581	the synthesized chitosan composite particles	538:581	the synthesized chitosan composite particles	538:581	The diameter of the synthesized chitosan composite particles ranged from 1.7 mm to 2.5 mm, and the embedded silver nanoparticles were measured to be 15 ± 3.3 nm.
25878501	3	15	theme	present	271:277	arg1	article					279:285	The present article	267:285	The present article	267:285	The present article describes a novel approach for one-step synthesis of silver nanoparticles-embedded chitosan particles.
25878501	6	16	theme	prepared	831:838	arg1	composites					840:849	the prepared composites	827:849	the prepared composites	827:849	Further, the analyses of ultraviolet-visible spectroscopy, energy dispersive spectroscopy, and X-ray diffraction were employed to characterize the prepared composites.
25878501	5	17	theme	chitosan	554:561	arg1	particles					573:581	the synthesized chitosan composite particles	538:581	the synthesized chitosan composite particles	538:581	The diameter of the synthesized chitosan composite particles ranged from 1.7 mm to 2.5 mm, and the embedded silver nanoparticles were measured to be 15 ± 3.3 nm.
25878501	7	18	dep	surface	925:931	arg1	the					921:923	the	921:923	the	921:923	The results show that the silver nanoparticles were distributed over the surface and interior of the chitosan spheres.
25878501	5	19	theme	composite	563:571	arg1	particles					573:581	the synthesized chitosan composite particles	538:581	the synthesized chitosan composite particles	538:581	The diameter of the synthesized chitosan composite particles ranged from 1.7 mm to 2.5 mm, and the embedded silver nanoparticles were measured to be 15 ± 3.3 nm.
25878501	5	20	theme	±	674:674	arg1	nm					680:681	15 ± 3.3 nm	671:681	15 ± 3.3 nm	671:681	The diameter of the synthesized chitosan composite particles ranged from 1.7 mm to 2.5 mm, and the embedded silver nanoparticles were measured to be 15 ± 3.3 nm.
25878501	5	20	theme	±	674:674	arg1	nanoparticles					637:649	the embedded silver nanoparticles	617:649	the embedded silver nanoparticles	617:649	The diameter of the synthesized chitosan composite particles ranged from 1.7 mm to 2.5 mm, and the embedded silver nanoparticles were measured to be 15 ± 3.3 nm.
25878501	8	21	from	agents	1079:1084	arg1	future					1093:1098	future	1093:1098	future	1093:1098	The fabricated spheres had macroporous property, and could be used for many applications such as fungicidal agents in the future.
25878501	5	22	theme	embedded	621:628	arg1	nanoparticles					637:649	the embedded silver nanoparticles	617:649	the embedded silver nanoparticles	617:649	The diameter of the synthesized chitosan composite particles ranged from 1.7 mm to 2.5 mm, and the embedded silver nanoparticles were measured to be 15 ± 3.3 nm.
25878501	5	22	theme	embedded	621:628	arg1	nm					680:681	15 ± 3.3 nm	671:681	15 ± 3.3 nm	671:681	The diameter of the synthesized chitosan composite particles ranged from 1.7 mm to 2.5 mm, and the embedded silver nanoparticles were measured to be 15 ± 3.3 nm.
25878501	7	23	theme	silver	878:883	arg1	nanoparticles					885:897	the silver nanoparticles	874:897	the silver nanoparticles	874:897	The results show that the silver nanoparticles were distributed over the surface and interior of the chitosan spheres.
25878501	0	24	theme	anti-fungal	14:24	arg1	effect					26:31	anti-fungal effect	14:31	anti-fungal effect	14:31	Synthesis and anti-fungal effect of silver nanoparticles-chitosan composite particles.
25878501	8	25	contain	had	994:996	arg1	spheres					986:992	The fabricated spheres	971:992	The fabricated spheres	971:992	The fabricated spheres had macroporous property, and could be used for many applications such as fungicidal agents in the future.
25878501	8	25	contain	had	994:996	arg2	property					1010:1017	macroporous property	998:1017	macroporous property	998:1017	The fabricated spheres had macroporous property, and could be used for many applications such as fungicidal agents in the future.
25878501	3	26	theme	novel	299:303	arg1	approach					305:312	a novel approach	297:312	a novel approach for one-step synthesis of silver nanoparticles-embedded chitosan particles	297:387	The present article describes a novel approach for one-step synthesis of silver nanoparticles-embedded chitosan particles.
25878501	4	27	theme	polymer	498:504	arg1	matrix					506:511	a chitosan polymer matrix	487:511	a chitosan polymer matrix in-situ	487:519	The proposed approach was applied to simultaneously obtain and stabilize silver nanoparticles in a chitosan polymer matrix in-situ.
25878501	0	28	theme	silver	36:41	arg1	particles					76:84	silver nanoparticles-chitosan composite particles	36:84	silver nanoparticles-chitosan composite particles	36:84	Synthesis and anti-fungal effect of silver nanoparticles-chitosan composite particles.
25878501	8	29	used	used	1033:1036	arg2	spheres					986:992	The fabricated spheres	971:992	The fabricated spheres	971:992	The fabricated spheres had macroporous property, and could be used for many applications such as fungicidal agents in the future.
25878501	4	30	theme	chitosan	489:496	arg1	matrix					506:511	a chitosan polymer matrix	487:511	a chitosan polymer matrix in-situ	487:519	The proposed approach was applied to simultaneously obtain and stabilize silver nanoparticles in a chitosan polymer matrix in-situ.
25878501	2	31	theme	nanoparticles	243:255	arg1	dispersion					213:222	dispersion	213:222	dispersion	213:222	The stability and dispersion of the synthesized nanoparticles is vital.
25878501	2	31	theme	nanoparticles	243:255	arg1	stability					199:207	stability	199:207	stability	199:207	The stability and dispersion of the synthesized nanoparticles is vital.
25878501	0	32	theme	composite	66:74	arg1	particles					76:84	silver nanoparticles-chitosan composite particles	36:84	silver nanoparticles-chitosan composite particles	36:84	Synthesis and anti-fungal effect of silver nanoparticles-chitosan composite particles.
25878501	3	33	theme	one-step	318:325	arg1	synthesis					327:335	one-step synthesis	318:335	one-step synthesis of silver nanoparticles-embedded chitosan particles	318:387	The present article describes a novel approach for one-step synthesis of silver nanoparticles-embedded chitosan particles.
25878501	0	34	theme	particles	76:84	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis and anti-fungal effect of silver nanoparticles-chitosan composite particles.
25878501	0	34	theme	particles	76:84	arg1	effect					26:31	anti-fungal effect	14:31	anti-fungal effect	14:31	Synthesis and anti-fungal effect of silver nanoparticles-chitosan composite particles.
25878501	2	35	theme	synthesized	231:241	arg1	nanoparticles					243:255	the synthesized nanoparticles	227:255	the synthesized nanoparticles	227:255	The stability and dispersion of the synthesized nanoparticles is vital.
25878501	1	36	theme	various	126:132	arg1	fields					134:139	various fields	126:139	various fields	126:139	Silver nanoparticles have been used in various fields, and several synthesis processes have been developed.
25878501	0	37	theme	nanoparticles-chitosan	43:64	arg1	particles					76:84	silver nanoparticles-chitosan composite particles	36:84	silver nanoparticles-chitosan composite particles	36:84	Synthesis and anti-fungal effect of silver nanoparticles-chitosan composite particles.
25878501	4	38	theme	proposed	394:401	arg1	approach					403:410	The proposed approach	390:410	The proposed approach	390:410	The proposed approach was applied to simultaneously obtain and stabilize silver nanoparticles in a chitosan polymer matrix in-situ.
25878501	1	39	used	used	118:121	arg2	nanoparticles					94:106	Silver nanoparticles	87:106	Silver nanoparticles	87:106	Silver nanoparticles have been used in various fields, and several synthesis processes have been developed.
25878501	7	40	theme	chitosan	953:960	arg1	spheres					962:968	the chitosan spheres	949:968	the chitosan spheres	949:968	The results show that the silver nanoparticles were distributed over the surface and interior of the chitosan spheres.
25878501	4	41	theme	silver	463:468	arg1	nanoparticles					470:482	silver nanoparticles	463:482	silver nanoparticles	463:482	The proposed approach was applied to simultaneously obtain and stabilize silver nanoparticles in a chitosan polymer matrix in-situ.
25878501	6	42	theme	X-ray	779:783	arg1	diffraction					785:795	X-ray diffraction	779:795	X-ray diffraction	779:795	Further, the analyses of ultraviolet-visible spectroscopy, energy dispersive spectroscopy, and X-ray diffraction were employed to characterize the prepared composites.
25878501	7	43	theme	spheres	962:968	arg1	interior					937:944	interior	937:944	interior	937:944	The results show that the silver nanoparticles were distributed over the surface and interior of the chitosan spheres.
25878501	7	43	theme	spheres	962:968	arg1	surface					925:931	surface	925:931	surface	925:931	The results show that the silver nanoparticles were distributed over the surface and interior of the chitosan spheres.
25878501	5	44	theme	2.5	605:607	arg1	mm					599:600	mm	599:600	mm	599:600	The diameter of the synthesized chitosan composite particles ranged from 1.7 mm to 2.5 mm, and the embedded silver nanoparticles were measured to be 15 ± 3.3 nm.
25878501	8	45	theme	fabricated	975:984	arg1	spheres					986:992	The fabricated spheres	971:992	The fabricated spheres	971:992	The fabricated spheres had macroporous property, and could be used for many applications such as fungicidal agents in the future.
25878501	5	46	theme	particles	573:581	arg1	diameter					526:533	The diameter	522:533	The diameter of the synthesized chitosan composite particles	522:581	The diameter of the synthesized chitosan composite particles ranged from 1.7 mm to 2.5 mm, and the embedded silver nanoparticles were measured to be 15 ± 3.3 nm.
25878501	2	47	dep	stability	199:207	arg1	The					195:197	The	195:197	The	195:197	The stability and dispersion of the synthesized nanoparticles is vital.
25878501	3	48	theme	nanoparticles-embedded	347:368	arg1	particles					379:387	silver nanoparticles-embedded chitosan particles	340:387	silver nanoparticles-embedded chitosan particles	340:387	The present article describes a novel approach for one-step synthesis of silver nanoparticles-embedded chitosan particles.
25878501	1	49	theme	several	146:152	arg1	processes					164:172	several synthesis processes	146:172	several synthesis processes	146:172	Silver nanoparticles have been used in various fields, and several synthesis processes have been developed.
25878501	3	50	theme	chitosan	370:377	arg1	particles					379:387	silver nanoparticles-embedded chitosan particles	340:387	silver nanoparticles-embedded chitosan particles	340:387	The present article describes a novel approach for one-step synthesis of silver nanoparticles-embedded chitosan particles.
25878501	6	51	theme	spectroscopy	761:772	arg1	analyses					697:704	the analyses	693:704	the analyses of ultraviolet-visible spectroscopy, energy dispersive spectroscopy, and X-ray diffraction	693:795	Further, the analyses of ultraviolet-visible spectroscopy, energy dispersive spectroscopy, and X-ray diffraction were employed to characterize the prepared composites.
25878501	1	52	theme	synthesis	154:162	arg1	processes					164:172	several synthesis processes	146:172	several synthesis processes	146:172	Silver nanoparticles have been used in various fields, and several synthesis processes have been developed.
27326857	1	0	theme	strain	471:476	arg1	capsule					435:441	the capsule	431:441	the capsule of Francisella live vaccine strain (LVS)	431:482	To pursue the possibility that the capsule of Francisella live vaccine strain (LVS) has a structurally unique lipid anchor, we have metabolically labeled Francisella with [14C]acetate to facilitate highly sensitive compositional analysis of capsule-associated lipids.
27326857	0	1	theme	same	368:371	arg1	tetrasaccharide					383:397	the same O-antigen tetrasaccharide	364:397	the same O-antigen tetrasaccharide	364:397	Evidence Suggesting That Francisella tularensis O-Antigen Capsule Contains a Lipid A-Like Molecule That Is Structurally Distinct from the More Abundant Free Lipid A. Francisella tularensis, the Gram-negative bacterium that causes tularemia, produces a high molecular weight capsule that is immunologically distinct from Francisella lipopolysaccharide but contains the same O-antigen tetrasaccharide.
27326857	3	2	theme	immunologic	766:776	arg1	analysis					778:785	Autoradiographic and immunologic analysis	745:785	Autoradiographic and immunologic analysis	745:785	Autoradiographic and immunologic analysis confirmed that this purified material was largely devoid of low molecular weight LPS and of the copious amounts of free lipid A that the Francisellae accumulate.
27326857	4	3	theme	fatty	1153:1157	arg1	acids					1159:1163	non-hydroxylated fatty acids	1136:1163	non-hydroxylated fatty acids (mainly C14:0 rather than C16:0)	1136:1196	Chemical hydrolysis yielded [14C]-labeled free fatty acids characteristic of Francisella lipid A but with a different molar ratio of 3-OH C18:0 to 3-OH C16:0 and different composition of non-hydroxylated fatty acids (mainly C14:0 rather than C16:0) than that of free Francisella lipid A. Mild acid hydrolysis to induce selective cleavage of KDO-lipid A linkage yielded a [14C]-labeled product that partitioned during Bligh/Dyer extraction and migrated during thin-layer chromatography like lipid A.
27326857	4	4	theme	free	991:994	arg1	acids					1002:1006	[14C]-labeled free fatty acids	977:1006	[14C]-labeled free fatty acids characteristic of Francisella lipid A but with a different molar ratio of 3-OH C18:0 to 3-OH C16:0 and different composition of non-hydroxylated fatty acids (mainly C14:0 rather than C16:0)	977:1196	Chemical hydrolysis yielded [14C]-labeled free fatty acids characteristic of Francisella lipid A but with a different molar ratio of 3-OH C18:0 to 3-OH C16:0 and different composition of non-hydroxylated fatty acids (mainly C14:0 rather than C16:0) than that of free Francisella lipid A. Mild acid hydrolysis to induce selective cleavage of KDO-lipid A linkage yielded a [14C]-labeled product that partitioned during Bligh/Dyer extraction and migrated during thin-layer chromatography like lipid A.
27326857	0	5	from	lipopolysaccharide	332:349	arg1	distinct					306:313	distinct	306:313	distinct	306:313	Evidence Suggesting That Francisella tularensis O-Antigen Capsule Contains a Lipid A-Like Molecule That Is Structurally Distinct from the More Abundant Free Lipid A. Francisella tularensis, the Gram-negative bacterium that causes tularemia, produces a high molecular weight capsule that is immunologically distinct from Francisella lipopolysaccharide but contains the same O-antigen tetrasaccharide.
27326857	4	6	theme	thin-layer	1408:1417	arg1	chromatography					1419:1432	thin-layer chromatography	1408:1432	thin-layer chromatography like lipid A	1408:1445	Chemical hydrolysis yielded [14C]-labeled free fatty acids characteristic of Francisella lipid A but with a different molar ratio of 3-OH C18:0 to 3-OH C16:0 and different composition of non-hydroxylated fatty acids (mainly C14:0 rather than C16:0) than that of free Francisella lipid A. Mild acid hydrolysis to induce selective cleavage of KDO-lipid A linkage yielded a [14C]-labeled product that partitioned during Bligh/Dyer extraction and migrated during thin-layer chromatography like lipid A.
27326857	4	7	theme	Chemical	949:956	arg1	hydrolysis					958:967	Chemical hydrolysis	949:967	Chemical hydrolysis	949:967	Chemical hydrolysis yielded [14C]-labeled free fatty acids characteristic of Francisella lipid A but with a different molar ratio of 3-OH C18:0 to 3-OH C16:0 and different composition of non-hydroxylated fatty acids (mainly C14:0 rather than C16:0) than that of free Francisella lipid A. Mild acid hydrolysis to induce selective cleavage of KDO-lipid A linkage yielded a [14C]-labeled product that partitioned during Bligh/Dyer extraction and migrated during thin-layer chromatography like lipid A.
27326857	4	8	theme	free	1211:1214	arg1	lipid					1228:1232	free Francisella lipid	1211:1232	free Francisella lipid	1211:1232	Chemical hydrolysis yielded [14C]-labeled free fatty acids characteristic of Francisella lipid A but with a different molar ratio of 3-OH C18:0 to 3-OH C16:0 and different composition of non-hydroxylated fatty acids (mainly C14:0 rather than C16:0) than that of free Francisella lipid A. Mild acid hydrolysis to induce selective cleavage of KDO-lipid A linkage yielded a [14C]-labeled product that partitioned during Bligh/Dyer extraction and migrated during thin-layer chromatography like lipid A.
27326857	0	9	theme	Free	152:155	arg1	tularensis					178:187	the More Abundant Free Lipid A. Francisella tularensis	134:187	the More Abundant Free Lipid A. Francisella tularensis	134:187	Evidence Suggesting That Francisella tularensis O-Antigen Capsule Contains a Lipid A-Like Molecule That Is Structurally Distinct from the More Abundant Free Lipid A. Francisella tularensis, the Gram-negative bacterium that causes tularemia, produces a high molecular weight capsule that is immunologically distinct from Francisella lipopolysaccharide but contains the same O-antigen tetrasaccharide.
27326857	0	9	theme	Free	152:155	arg1	bacterium					208:216	the Gram-negative bacterium	190:216	the Gram-negative bacterium that causes tularemia, produces a high molecular weight capsule that is immunologically distinct from Francisella lipopolysaccharide but contains the same O-antigen tetrasaccharide	190:397	Evidence Suggesting That Francisella tularensis O-Antigen Capsule Contains a Lipid A-Like Molecule That Is Structurally Distinct from the More Abundant Free Lipid A. Francisella tularensis, the Gram-negative bacterium that causes tularemia, produces a high molecular weight capsule that is immunologically distinct from Francisella lipopolysaccharide but contains the same O-antigen tetrasaccharide.
27326857	4	10	dep	acids	1159:1163	arg1	C14:0					1173:1177	C14:0	1173:1177	C14:0	1173:1177	Chemical hydrolysis yielded [14C]-labeled free fatty acids characteristic of Francisella lipid A but with a different molar ratio of 3-OH C18:0 to 3-OH C16:0 and different composition of non-hydroxylated fatty acids (mainly C14:0 rather than C16:0) than that of free Francisella lipid A. Mild acid hydrolysis to induce selective cleavage of KDO-lipid A linkage yielded a [14C]-labeled product that partitioned during Bligh/Dyer extraction and migrated during thin-layer chromatography like lipid A.
27326857	4	10	dep	acids	1159:1163	arg1	C16:0					1191:1195	C16:0	1191:1195	C16:0	1191:1195	Chemical hydrolysis yielded [14C]-labeled free fatty acids characteristic of Francisella lipid A but with a different molar ratio of 3-OH C18:0 to 3-OH C16:0 and different composition of non-hydroxylated fatty acids (mainly C14:0 rather than C16:0) than that of free Francisella lipid A. Mild acid hydrolysis to induce selective cleavage of KDO-lipid A linkage yielded a [14C]-labeled product that partitioned during Bligh/Dyer extraction and migrated during thin-layer chromatography like lipid A.
27326857	3	11	theme	lipid	907:911	arg1	A					913:913	free lipid A	902:913	free lipid A	902:913	Autoradiographic and immunologic analysis confirmed that this purified material was largely devoid of low molecular weight LPS and of the copious amounts of free lipid A that the Francisellae accumulate.
27326857	3	12	theme	LPS	868:870	arg1	devoid					837:842	devoid	837:842	devoid	837:842	Autoradiographic and immunologic analysis confirmed that this purified material was largely devoid of low molecular weight LPS and of the copious amounts of free lipid A that the Francisellae accumulate.
27326857	0	13	theme	A.	163:164	arg1	tularensis					178:187	the More Abundant Free Lipid A. Francisella tularensis	134:187	the More Abundant Free Lipid A. Francisella tularensis	134:187	Evidence Suggesting That Francisella tularensis O-Antigen Capsule Contains a Lipid A-Like Molecule That Is Structurally Distinct from the More Abundant Free Lipid A. Francisella tularensis, the Gram-negative bacterium that causes tularemia, produces a high molecular weight capsule that is immunologically distinct from Francisella lipopolysaccharide but contains the same O-antigen tetrasaccharide.
27326857	0	13	theme	A.	163:164	arg1	bacterium					208:216	the Gram-negative bacterium	190:216	the Gram-negative bacterium that causes tularemia, produces a high molecular weight capsule that is immunologically distinct from Francisella lipopolysaccharide but contains the same O-antigen tetrasaccharide	190:397	Evidence Suggesting That Francisella tularensis O-Antigen Capsule Contains a Lipid A-Like Molecule That Is Structurally Distinct from the More Abundant Free Lipid A. Francisella tularensis, the Gram-negative bacterium that causes tularemia, produces a high molecular weight capsule that is immunologically distinct from Francisella lipopolysaccharide but contains the same O-antigen tetrasaccharide.
27326857	1	14	theme	live	458:461	arg1	LVS					479:481	LVS	479:481	LVS	479:481	To pursue the possibility that the capsule of Francisella live vaccine strain (LVS) has a structurally unique lipid anchor, we have metabolically labeled Francisella with [14C]acetate to facilitate highly sensitive compositional analysis of capsule-associated lipids.
27326857	1	14	theme	live	458:461	arg1	strain					471:476	Francisella live vaccine strain	446:476	Francisella live vaccine strain (LVS)	446:482	To pursue the possibility that the capsule of Francisella live vaccine strain (LVS) has a structurally unique lipid anchor, we have metabolically labeled Francisella with [14C]acetate to facilitate highly sensitive compositional analysis of capsule-associated lipids.
27326857	4	15	theme	lipid	1038:1042	arg1	A					1044:1044	Francisella lipid A	1026:1044	Francisella lipid A	1026:1044	Chemical hydrolysis yielded [14C]-labeled free fatty acids characteristic of Francisella lipid A but with a different molar ratio of 3-OH C18:0 to 3-OH C16:0 and different composition of non-hydroxylated fatty acids (mainly C14:0 rather than C16:0) than that of free Francisella lipid A. Mild acid hydrolysis to induce selective cleavage of KDO-lipid A linkage yielded a [14C]-labeled product that partitioned during Bligh/Dyer extraction and migrated during thin-layer chromatography like lipid A.
27326857	1	16	theme	capsule-associated	641:658	arg1	lipids					660:665	capsule-associated lipids	641:665	capsule-associated lipids	641:665	To pursue the possibility that the capsule of Francisella live vaccine strain (LVS) has a structurally unique lipid anchor, we have metabolically labeled Francisella with [14C]acetate to facilitate highly sensitive compositional analysis of capsule-associated lipids.
27326857	3	17	theme	molecular	851:859	arg1	weight					861:866	low molecular weight	847:866	low molecular weight LPS	847:870	Autoradiographic and immunologic analysis confirmed that this purified material was largely devoid of low molecular weight LPS and of the copious amounts of free lipid A that the Francisellae accumulate.
27326857	6	18	theme	A-like	1618:1623	arg1	molecule					1625:1632	a discrete lipid A-like molecule	1601:1632	a discrete lipid A-like molecule associated with capsule	1601:1656	The presence of a discrete lipid A-like molecule associated with capsule raises the possibility that Francisella selectively exploits lipid A structural heterogeneity to regulate synthesis, transport, and stable bacterial surface association of the O-antigen capsular layer.
27326857	6	19	theme	O-antigen	1834:1842	arg1	layer					1853:1857	the O-antigen capsular layer	1830:1857	the O-antigen capsular layer	1830:1857	The presence of a discrete lipid A-like molecule associated with capsule raises the possibility that Francisella selectively exploits lipid A structural heterogeneity to regulate synthesis, transport, and stable bacterial surface association of the O-antigen capsular layer.
27326857	3	20	theme	copious	883:889	arg1	A					913:913	free lipid A	902:913	free lipid A	902:913	Autoradiographic and immunologic analysis confirmed that this purified material was largely devoid of low molecular weight LPS and of the copious amounts of free lipid A that the Francisellae accumulate.
27326857	3	20	theme	copious	883:889	arg1	amounts					891:897	the copious amounts	879:897	the copious amounts of free lipid A	879:913	Autoradiographic and immunologic analysis confirmed that this purified material was largely devoid of low molecular weight LPS and of the copious amounts of free lipid A that the Francisellae accumulate.
27326857	0	21	theme	A-Like	83:88	arg1	Molecule					90:97	a Lipid A-Like Molecule	75:97	a Lipid A-Like Molecule That Is Structurally Distinct from the More Abundant Free Lipid A. Francisella tularensis, the Gram-negative bacterium that causes tularemia, produces a high molecular weight capsule that is immunologically distinct from Francisella lipopolysaccharide but contains the same O-antigen tetrasaccharide	75:397	Evidence Suggesting That Francisella tularensis O-Antigen Capsule Contains a Lipid A-Like Molecule That Is Structurally Distinct from the More Abundant Free Lipid A. Francisella tularensis, the Gram-negative bacterium that causes tularemia, produces a high molecular weight capsule that is immunologically distinct from Francisella lipopolysaccharide but contains the same O-antigen tetrasaccharide.
27326857	6	22	theme	structural	1727:1736	arg1	heterogeneity					1738:1750	structural heterogeneity	1727:1750	structural heterogeneity	1727:1750	The presence of a discrete lipid A-like molecule associated with capsule raises the possibility that Francisella selectively exploits lipid A structural heterogeneity to regulate synthesis, transport, and stable bacterial surface association of the O-antigen capsular layer.
27326857	5	23	theme	distinct	1559:1566	arg1	species					1576:1582	a covalently linked and structurally distinct lipid A species	1522:1582	a covalently linked and structurally distinct lipid A species	1522:1582	These findings suggest that the O-antigen capsule of Francisella contains a covalently linked and structurally distinct lipid A species.
27326857	0	24	theme	high	252:255	arg1	weight					267:272	a high molecular weight	250:272	a high molecular weight capsule that is immunologically distinct from Francisella lipopolysaccharide	250:349	Evidence Suggesting That Francisella tularensis O-Antigen Capsule Contains a Lipid A-Like Molecule That Is Structurally Distinct from the More Abundant Free Lipid A. Francisella tularensis, the Gram-negative bacterium that causes tularemia, produces a high molecular weight capsule that is immunologically distinct from Francisella lipopolysaccharide but contains the same O-antigen tetrasaccharide.
27326857	6	25	theme	surface	1807:1813	arg1	association					1815:1825	stable bacterial surface association	1790:1825	stable bacterial surface association	1790:1825	The presence of a discrete lipid A-like molecule associated with capsule raises the possibility that Francisella selectively exploits lipid A structural heterogeneity to regulate synthesis, transport, and stable bacterial surface association of the O-antigen capsular layer.
27326857	0	26	theme	weight	267:272	arg1	capsule					274:280	a high molecular weight capsule	250:280	a high molecular weight capsule that is immunologically distinct from Francisella lipopolysaccharide	250:349	Evidence Suggesting That Francisella tularensis O-Antigen Capsule Contains a Lipid A-Like Molecule That Is Structurally Distinct from the More Abundant Free Lipid A. Francisella tularensis, the Gram-negative bacterium that causes tularemia, produces a high molecular weight capsule that is immunologically distinct from Francisella lipopolysaccharide but contains the same O-antigen tetrasaccharide.
27326857	6	27	theme	stable	1790:1795	arg1	association					1815:1825	stable bacterial surface association	1790:1825	stable bacterial surface association	1790:1825	The presence of a discrete lipid A-like molecule associated with capsule raises the possibility that Francisella selectively exploits lipid A structural heterogeneity to regulate synthesis, transport, and stable bacterial surface association of the O-antigen capsular layer.
27326857	4	28	theme	Mild	1237:1240	arg1	hydrolysis					1247:1256	Mild acid hydrolysis	1237:1256	Mild acid hydrolysis to induce selective cleavage of KDO-lipid A linkage yielded a [14C]-labeled product that partitioned during Bligh/Dyer extraction and migrated during thin-layer chromatography like lipid A	1237:1445	Chemical hydrolysis yielded [14C]-labeled free fatty acids characteristic of Francisella lipid A but with a different molar ratio of 3-OH C18:0 to 3-OH C16:0 and different composition of non-hydroxylated fatty acids (mainly C14:0 rather than C16:0) than that of free Francisella lipid A. Mild acid hydrolysis to induce selective cleavage of KDO-lipid A linkage yielded a [14C]-labeled product that partitioned during Bligh/Dyer extraction and migrated during thin-layer chromatography like lipid A.
27326857	4	29	theme	C18:0	1087:1091	arg1	ratio					1073:1077	a different molar ratio	1055:1077	a different molar ratio of 3-OH C18:0 to 3-OH C16:0 and different composition of non-hydroxylated fatty acids (mainly C14:0 rather than C16:0)	1055:1196	Chemical hydrolysis yielded [14C]-labeled free fatty acids characteristic of Francisella lipid A but with a different molar ratio of 3-OH C18:0 to 3-OH C16:0 and different composition of non-hydroxylated fatty acids (mainly C14:0 rather than C16:0) than that of free Francisella lipid A. Mild acid hydrolysis to induce selective cleavage of KDO-lipid A linkage yielded a [14C]-labeled product that partitioned during Bligh/Dyer extraction and migrated during thin-layer chromatography like lipid A.
27326857	6	30	theme	molecule	1625:1632	arg1	presence					1589:1596	The presence	1585:1596	The presence of a discrete lipid A-like molecule associated with capsule	1585:1656	The presence of a discrete lipid A-like molecule associated with capsule raises the possibility that Francisella selectively exploits lipid A structural heterogeneity to regulate synthesis, transport, and stable bacterial surface association of the O-antigen capsular layer.
27326857	0	31	from	tularensis	178:187	arg1	Distinct					120:127	Distinct	120:127	Distinct	120:127	Evidence Suggesting That Francisella tularensis O-Antigen Capsule Contains a Lipid A-Like Molecule That Is Structurally Distinct from the More Abundant Free Lipid A. Francisella tularensis, the Gram-negative bacterium that causes tularemia, produces a high molecular weight capsule that is immunologically distinct from Francisella lipopolysaccharide but contains the same O-antigen tetrasaccharide.
27326857	1	32	theme	sensitive	605:613	arg1	analysis					629:636	highly sensitive compositional analysis	598:636	highly sensitive compositional analysis of capsule-associated lipids	598:665	To pursue the possibility that the capsule of Francisella live vaccine strain (LVS) has a structurally unique lipid anchor, we have metabolically labeled Francisella with [14C]acetate to facilitate highly sensitive compositional analysis of capsule-associated lipids.
27326857	0	33	theme	tularensis	37:46	arg1	Capsule					58:64	Francisella tularensis O-Antigen Capsule	25:64	Francisella tularensis O-Antigen Capsule	25:64	Evidence Suggesting That Francisella tularensis O-Antigen Capsule Contains a Lipid A-Like Molecule That Is Structurally Distinct from the More Abundant Free Lipid A. Francisella tularensis, the Gram-negative bacterium that causes tularemia, produces a high molecular weight capsule that is immunologically distinct from Francisella lipopolysaccharide but contains the same O-antigen tetrasaccharide.
27326857	4	34	theme	molar	1067:1071	arg1	ratio					1073:1077	a different molar ratio	1055:1077	a different molar ratio of 3-OH C18:0 to 3-OH C16:0 and different composition of non-hydroxylated fatty acids (mainly C14:0 rather than C16:0)	1055:1196	Chemical hydrolysis yielded [14C]-labeled free fatty acids characteristic of Francisella lipid A but with a different molar ratio of 3-OH C18:0 to 3-OH C16:0 and different composition of non-hydroxylated fatty acids (mainly C14:0 rather than C16:0) than that of free Francisella lipid A. Mild acid hydrolysis to induce selective cleavage of KDO-lipid A linkage yielded a [14C]-labeled product that partitioned during Bligh/Dyer extraction and migrated during thin-layer chromatography like lipid A.
27326857	5	35	theme	A	1574:1574	arg1	species					1576:1582	a covalently linked and structurally distinct lipid A species	1522:1582	a covalently linked and structurally distinct lipid A species	1522:1582	These findings suggest that the O-antigen capsule of Francisella contains a covalently linked and structurally distinct lipid A species.
27326857	6	36	theme	layer	1853:1857	arg1	transport					1775:1783	transport	1775:1783	transport	1775:1783	The presence of a discrete lipid A-like molecule associated with capsule raises the possibility that Francisella selectively exploits lipid A structural heterogeneity to regulate synthesis, transport, and stable bacterial surface association of the O-antigen capsular layer.
27326857	6	36	theme	layer	1853:1857	arg1	synthesis					1764:1772	synthesis	1764:1772	synthesis	1764:1772	The presence of a discrete lipid A-like molecule associated with capsule raises the possibility that Francisella selectively exploits lipid A structural heterogeneity to regulate synthesis, transport, and stable bacterial surface association of the O-antigen capsular layer.
27326857	6	36	theme	layer	1853:1857	arg1	association					1815:1825	stable bacterial surface association	1790:1825	stable bacterial surface association	1790:1825	The presence of a discrete lipid A-like molecule associated with capsule raises the possibility that Francisella selectively exploits lipid A structural heterogeneity to regulate synthesis, transport, and stable bacterial surface association of the O-antigen capsular layer.
27326857	4	37	theme	KDO-lipid	1290:1298	arg1	linkage					1302:1308	KDO-lipid A linkage	1290:1308	KDO-lipid A linkage yielded a [14C]-labeled product that partitioned during Bligh/Dyer extraction and migrated during thin-layer chromatography like lipid A	1290:1445	Chemical hydrolysis yielded [14C]-labeled free fatty acids characteristic of Francisella lipid A but with a different molar ratio of 3-OH C18:0 to 3-OH C16:0 and different composition of non-hydroxylated fatty acids (mainly C14:0 rather than C16:0) than that of free Francisella lipid A. Mild acid hydrolysis to induce selective cleavage of KDO-lipid A linkage yielded a [14C]-labeled product that partitioned during Bligh/Dyer extraction and migrated during thin-layer chromatography like lipid A.
27326857	4	38	dep	hydrolysis	1247:1256	arg1	A.					1234:1235	A.	1234:1235	A.	1234:1235	Chemical hydrolysis yielded [14C]-labeled free fatty acids characteristic of Francisella lipid A but with a different molar ratio of 3-OH C18:0 to 3-OH C16:0 and different composition of non-hydroxylated fatty acids (mainly C14:0 rather than C16:0) than that of free Francisella lipid A. Mild acid hydrolysis to induce selective cleavage of KDO-lipid A linkage yielded a [14C]-labeled product that partitioned during Bligh/Dyer extraction and migrated during thin-layer chromatography like lipid A.
27326857	4	39	theme	non-hydroxylated	1136:1151	arg1	acids					1159:1163	non-hydroxylated fatty acids	1136:1163	non-hydroxylated fatty acids (mainly C14:0 rather than C16:0)	1136:1196	Chemical hydrolysis yielded [14C]-labeled free fatty acids characteristic of Francisella lipid A but with a different molar ratio of 3-OH C18:0 to 3-OH C16:0 and different composition of non-hydroxylated fatty acids (mainly C14:0 rather than C16:0) than that of free Francisella lipid A. Mild acid hydrolysis to induce selective cleavage of KDO-lipid A linkage yielded a [14C]-labeled product that partitioned during Bligh/Dyer extraction and migrated during thin-layer chromatography like lipid A.
27326857	1	40	with	Francisella	554:564	arg1	acetate					576:582	[14C]acetate	571:582	[14C]acetate	571:582	To pursue the possibility that the capsule of Francisella live vaccine strain (LVS) has a structurally unique lipid anchor, we have metabolically labeled Francisella with [14C]acetate to facilitate highly sensitive compositional analysis of capsule-associated lipids.
27326857	4	41	theme	linkage	1302:1308	arg1	cleavage					1278:1285	selective cleavage	1268:1285	selective cleavage of KDO-lipid A linkage yielded a [14C]-labeled product that partitioned during Bligh/Dyer extraction and migrated during thin-layer chromatography like lipid A	1268:1445	Chemical hydrolysis yielded [14C]-labeled free fatty acids characteristic of Francisella lipid A but with a different molar ratio of 3-OH C18:0 to 3-OH C16:0 and different composition of non-hydroxylated fatty acids (mainly C14:0 rather than C16:0) than that of free Francisella lipid A. Mild acid hydrolysis to induce selective cleavage of KDO-lipid A linkage yielded a [14C]-labeled product that partitioned during Bligh/Dyer extraction and migrated during thin-layer chromatography like lipid A.
27326857	4	42	theme	3-OH	1096:1099	arg1	C16:0					1101:1105	3-OH C16:0	1096:1105	3-OH C16:0	1096:1105	Chemical hydrolysis yielded [14C]-labeled free fatty acids characteristic of Francisella lipid A but with a different molar ratio of 3-OH C18:0 to 3-OH C16:0 and different composition of non-hydroxylated fatty acids (mainly C14:0 rather than C16:0) than that of free Francisella lipid A. Mild acid hydrolysis to induce selective cleavage of KDO-lipid A linkage yielded a [14C]-labeled product that partitioned during Bligh/Dyer extraction and migrated during thin-layer chromatography like lipid A.
27326857	4	43	theme	Bligh/Dyer	1366:1375	arg1	extraction					1377:1386	Bligh/Dyer extraction	1366:1386	Bligh/Dyer extraction	1366:1386	Chemical hydrolysis yielded [14C]-labeled free fatty acids characteristic of Francisella lipid A but with a different molar ratio of 3-OH C18:0 to 3-OH C16:0 and different composition of non-hydroxylated fatty acids (mainly C14:0 rather than C16:0) than that of free Francisella lipid A. Mild acid hydrolysis to induce selective cleavage of KDO-lipid A linkage yielded a [14C]-labeled product that partitioned during Bligh/Dyer extraction and migrated during thin-layer chromatography like lipid A.
27326857	6	44	theme	discrete	1603:1610	arg1	molecule					1625:1632	a discrete lipid A-like molecule	1601:1632	a discrete lipid A-like molecule associated with capsule	1601:1656	The presence of a discrete lipid A-like molecule associated with capsule raises the possibility that Francisella selectively exploits lipid A structural heterogeneity to regulate synthesis, transport, and stable bacterial surface association of the O-antigen capsular layer.
27326857	0	45	theme	Gram-negative	194:206	arg1	tularensis					178:187	the More Abundant Free Lipid A. Francisella tularensis	134:187	the More Abundant Free Lipid A. Francisella tularensis	134:187	Evidence Suggesting That Francisella tularensis O-Antigen Capsule Contains a Lipid A-Like Molecule That Is Structurally Distinct from the More Abundant Free Lipid A. Francisella tularensis, the Gram-negative bacterium that causes tularemia, produces a high molecular weight capsule that is immunologically distinct from Francisella lipopolysaccharide but contains the same O-antigen tetrasaccharide.
27326857	0	45	theme	Gram-negative	194:206	arg1	bacterium					208:216	the Gram-negative bacterium	190:216	the Gram-negative bacterium that causes tularemia, produces a high molecular weight capsule that is immunologically distinct from Francisella lipopolysaccharide but contains the same O-antigen tetrasaccharide	190:397	Evidence Suggesting That Francisella tularensis O-Antigen Capsule Contains a Lipid A-Like Molecule That Is Structurally Distinct from the More Abundant Free Lipid A. Francisella tularensis, the Gram-negative bacterium that causes tularemia, produces a high molecular weight capsule that is immunologically distinct from Francisella lipopolysaccharide but contains the same O-antigen tetrasaccharide.
27326857	0	46	contain	Contains	66:73	arg2	Molecule					90:97	a Lipid A-Like Molecule	75:97	a Lipid A-Like Molecule That Is Structurally Distinct from the More Abundant Free Lipid A. Francisella tularensis, the Gram-negative bacterium that causes tularemia, produces a high molecular weight capsule that is immunologically distinct from Francisella lipopolysaccharide but contains the same O-antigen tetrasaccharide	75:397	Evidence Suggesting That Francisella tularensis O-Antigen Capsule Contains a Lipid A-Like Molecule That Is Structurally Distinct from the More Abundant Free Lipid A. Francisella tularensis, the Gram-negative bacterium that causes tularemia, produces a high molecular weight capsule that is immunologically distinct from Francisella lipopolysaccharide but contains the same O-antigen tetrasaccharide.
27326857	0	46	contain	Contains	66:73	arg1	Capsule					58:64	Francisella tularensis O-Antigen Capsule	25:64	Francisella tularensis O-Antigen Capsule	25:64	Evidence Suggesting That Francisella tularensis O-Antigen Capsule Contains a Lipid A-Like Molecule That Is Structurally Distinct from the More Abundant Free Lipid A. Francisella tularensis, the Gram-negative bacterium that causes tularemia, produces a high molecular weight capsule that is immunologically distinct from Francisella lipopolysaccharide but contains the same O-antigen tetrasaccharide.
27326857	5	47	theme	Francisella	1501:1511	arg1	capsule					1490:1496	the O-antigen capsule	1476:1496	the O-antigen capsule of Francisella	1476:1511	These findings suggest that the O-antigen capsule of Francisella contains a covalently linked and structurally distinct lipid A species.
27326857	1	48	contain	has	484:486	arg1	capsule					435:441	the capsule	431:441	the capsule of Francisella live vaccine strain (LVS)	431:482	To pursue the possibility that the capsule of Francisella live vaccine strain (LVS) has a structurally unique lipid anchor, we have metabolically labeled Francisella with [14C]acetate to facilitate highly sensitive compositional analysis of capsule-associated lipids.
27326857	1	48	contain	has	484:486	arg2	anchor					516:521	a structurally unique lipid anchor	488:521	a structurally unique lipid anchor	488:521	To pursue the possibility that the capsule of Francisella live vaccine strain (LVS) has a structurally unique lipid anchor, we have metabolically labeled Francisella with [14C]acetate to facilitate highly sensitive compositional analysis of capsule-associated lipids.
27326857	5	49	link	linked	1535:1540	arg1	species					1576:1582	a covalently linked and structurally distinct lipid A species	1522:1582	a covalently linked and structurally distinct lipid A species	1522:1582	These findings suggest that the O-antigen capsule of Francisella contains a covalently linked and structurally distinct lipid A species.
27326857	0	50	theme	O-antigen	373:381	arg1	tetrasaccharide					383:397	the same O-antigen tetrasaccharide	364:397	the same O-antigen tetrasaccharide	364:397	Evidence Suggesting That Francisella tularensis O-Antigen Capsule Contains a Lipid A-Like Molecule That Is Structurally Distinct from the More Abundant Free Lipid A. Francisella tularensis, the Gram-negative bacterium that causes tularemia, produces a high molecular weight capsule that is immunologically distinct from Francisella lipopolysaccharide but contains the same O-antigen tetrasaccharide.
27326857	4	51	theme	acids	1159:1163	arg1	C16:0					1101:1105	3-OH C16:0	1096:1105	3-OH C16:0	1096:1105	Chemical hydrolysis yielded [14C]-labeled free fatty acids characteristic of Francisella lipid A but with a different molar ratio of 3-OH C18:0 to 3-OH C16:0 and different composition of non-hydroxylated fatty acids (mainly C14:0 rather than C16:0) than that of free Francisella lipid A. Mild acid hydrolysis to induce selective cleavage of KDO-lipid A linkage yielded a [14C]-labeled product that partitioned during Bligh/Dyer extraction and migrated during thin-layer chromatography like lipid A.
27326857	4	51	theme	acids	1159:1163	arg1	composition					1121:1131	different composition	1111:1131	different composition	1111:1131	Chemical hydrolysis yielded [14C]-labeled free fatty acids characteristic of Francisella lipid A but with a different molar ratio of 3-OH C18:0 to 3-OH C16:0 and different composition of non-hydroxylated fatty acids (mainly C14:0 rather than C16:0) than that of free Francisella lipid A. Mild acid hydrolysis to induce selective cleavage of KDO-lipid A linkage yielded a [14C]-labeled product that partitioned during Bligh/Dyer extraction and migrated during thin-layer chromatography like lipid A.
27326857	4	52	theme	lipid	1439:1443	arg1	A					1445:1445	lipid A	1439:1445	lipid A	1439:1445	Chemical hydrolysis yielded [14C]-labeled free fatty acids characteristic of Francisella lipid A but with a different molar ratio of 3-OH C18:0 to 3-OH C16:0 and different composition of non-hydroxylated fatty acids (mainly C14:0 rather than C16:0) than that of free Francisella lipid A. Mild acid hydrolysis to induce selective cleavage of KDO-lipid A linkage yielded a [14C]-labeled product that partitioned during Bligh/Dyer extraction and migrated during thin-layer chromatography like lipid A.
27326857	1	53	theme	unique	503:508	arg1	anchor					516:521	a structurally unique lipid anchor	488:521	a structurally unique lipid anchor	488:521	To pursue the possibility that the capsule of Francisella live vaccine strain (LVS) has a structurally unique lipid anchor, we have metabolically labeled Francisella with [14C]acetate to facilitate highly sensitive compositional analysis of capsule-associated lipids.
27326857	4	54	theme	selective	1268:1276	arg1	cleavage					1278:1285	selective cleavage	1268:1285	selective cleavage of KDO-lipid A linkage yielded a [14C]-labeled product that partitioned during Bligh/Dyer extraction and migrated during thin-layer chromatography like lipid A	1268:1445	Chemical hydrolysis yielded [14C]-labeled free fatty acids characteristic of Francisella lipid A but with a different molar ratio of 3-OH C18:0 to 3-OH C16:0 and different composition of non-hydroxylated fatty acids (mainly C14:0 rather than C16:0) than that of free Francisella lipid A. Mild acid hydrolysis to induce selective cleavage of KDO-lipid A linkage yielded a [14C]-labeled product that partitioned during Bligh/Dyer extraction and migrated during thin-layer chromatography like lipid A.
27326857	3	55	theme	purified	807:814	arg1	material					816:823	this purified material	802:823	this purified material	802:823	Autoradiographic and immunologic analysis confirmed that this purified material was largely devoid of low molecular weight LPS and of the copious amounts of free lipid A that the Francisellae accumulate.
27326857	0	56	theme	Abundant	143:150	arg1	tularensis					178:187	the More Abundant Free Lipid A. Francisella tularensis	134:187	the More Abundant Free Lipid A. Francisella tularensis	134:187	Evidence Suggesting That Francisella tularensis O-Antigen Capsule Contains a Lipid A-Like Molecule That Is Structurally Distinct from the More Abundant Free Lipid A. Francisella tularensis, the Gram-negative bacterium that causes tularemia, produces a high molecular weight capsule that is immunologically distinct from Francisella lipopolysaccharide but contains the same O-antigen tetrasaccharide.
27326857	0	56	theme	Abundant	143:150	arg1	bacterium					208:216	the Gram-negative bacterium	190:216	the Gram-negative bacterium that causes tularemia, produces a high molecular weight capsule that is immunologically distinct from Francisella lipopolysaccharide but contains the same O-antigen tetrasaccharide	190:397	Evidence Suggesting That Francisella tularensis O-Antigen Capsule Contains a Lipid A-Like Molecule That Is Structurally Distinct from the More Abundant Free Lipid A. Francisella tularensis, the Gram-negative bacterium that causes tularemia, produces a high molecular weight capsule that is immunologically distinct from Francisella lipopolysaccharide but contains the same O-antigen tetrasaccharide.
27326857	4	57	theme	-labeled	982:989	arg1	acids					1002:1006	[14C]-labeled free fatty acids	977:1006	[14C]-labeled free fatty acids characteristic of Francisella lipid A but with a different molar ratio of 3-OH C18:0 to 3-OH C16:0 and different composition of non-hydroxylated fatty acids (mainly C14:0 rather than C16:0)	977:1196	Chemical hydrolysis yielded [14C]-labeled free fatty acids characteristic of Francisella lipid A but with a different molar ratio of 3-OH C18:0 to 3-OH C16:0 and different composition of non-hydroxylated fatty acids (mainly C14:0 rather than C16:0) than that of free Francisella lipid A. Mild acid hydrolysis to induce selective cleavage of KDO-lipid A linkage yielded a [14C]-labeled product that partitioned during Bligh/Dyer extraction and migrated during thin-layer chromatography like lipid A.
27326857	4	58	theme	Francisella	1216:1226	arg1	lipid					1228:1232	free Francisella lipid	1211:1232	free Francisella lipid	1211:1232	Chemical hydrolysis yielded [14C]-labeled free fatty acids characteristic of Francisella lipid A but with a different molar ratio of 3-OH C18:0 to 3-OH C16:0 and different composition of non-hydroxylated fatty acids (mainly C14:0 rather than C16:0) than that of free Francisella lipid A. Mild acid hydrolysis to induce selective cleavage of KDO-lipid A linkage yielded a [14C]-labeled product that partitioned during Bligh/Dyer extraction and migrated during thin-layer chromatography like lipid A.
27326857	3	59	theme	low	847:849	arg1	weight					861:866	low molecular weight	847:866	low molecular weight LPS	847:870	Autoradiographic and immunologic analysis confirmed that this purified material was largely devoid of low molecular weight LPS and of the copious amounts of free lipid A that the Francisellae accumulate.
27326857	0	60	theme	Lipid	157:161	arg1	tularensis					178:187	the More Abundant Free Lipid A. Francisella tularensis	134:187	the More Abundant Free Lipid A. Francisella tularensis	134:187	Evidence Suggesting That Francisella tularensis O-Antigen Capsule Contains a Lipid A-Like Molecule That Is Structurally Distinct from the More Abundant Free Lipid A. Francisella tularensis, the Gram-negative bacterium that causes tularemia, produces a high molecular weight capsule that is immunologically distinct from Francisella lipopolysaccharide but contains the same O-antigen tetrasaccharide.
27326857	0	60	theme	Lipid	157:161	arg1	bacterium					208:216	the Gram-negative bacterium	190:216	the Gram-negative bacterium that causes tularemia, produces a high molecular weight capsule that is immunologically distinct from Francisella lipopolysaccharide but contains the same O-antigen tetrasaccharide	190:397	Evidence Suggesting That Francisella tularensis O-Antigen Capsule Contains a Lipid A-Like Molecule That Is Structurally Distinct from the More Abundant Free Lipid A. Francisella tularensis, the Gram-negative bacterium that causes tularemia, produces a high molecular weight capsule that is immunologically distinct from Francisella lipopolysaccharide but contains the same O-antigen tetrasaccharide.
27326857	1	61	theme	compositional	615:627	arg1	analysis					629:636	highly sensitive compositional analysis	598:636	highly sensitive compositional analysis of capsule-associated lipids	598:665	To pursue the possibility that the capsule of Francisella live vaccine strain (LVS) has a structurally unique lipid anchor, we have metabolically labeled Francisella with [14C]acetate to facilitate highly sensitive compositional analysis of capsule-associated lipids.
27326857	0	62	theme	Francisella	166:176	arg1	tularensis					178:187	the More Abundant Free Lipid A. Francisella tularensis	134:187	the More Abundant Free Lipid A. Francisella tularensis	134:187	Evidence Suggesting That Francisella tularensis O-Antigen Capsule Contains a Lipid A-Like Molecule That Is Structurally Distinct from the More Abundant Free Lipid A. Francisella tularensis, the Gram-negative bacterium that causes tularemia, produces a high molecular weight capsule that is immunologically distinct from Francisella lipopolysaccharide but contains the same O-antigen tetrasaccharide.
27326857	0	62	theme	Francisella	166:176	arg1	bacterium					208:216	the Gram-negative bacterium	190:216	the Gram-negative bacterium that causes tularemia, produces a high molecular weight capsule that is immunologically distinct from Francisella lipopolysaccharide but contains the same O-antigen tetrasaccharide	190:397	Evidence Suggesting That Francisella tularensis O-Antigen Capsule Contains a Lipid A-Like Molecule That Is Structurally Distinct from the More Abundant Free Lipid A. Francisella tularensis, the Gram-negative bacterium that causes tularemia, produces a high molecular weight capsule that is immunologically distinct from Francisella lipopolysaccharide but contains the same O-antigen tetrasaccharide.
27326857	1	63	theme	Francisella	446:456	arg1	LVS					479:481	LVS	479:481	LVS	479:481	To pursue the possibility that the capsule of Francisella live vaccine strain (LVS) has a structurally unique lipid anchor, we have metabolically labeled Francisella with [14C]acetate to facilitate highly sensitive compositional analysis of capsule-associated lipids.
27326857	1	63	theme	Francisella	446:456	arg1	strain					471:476	Francisella live vaccine strain	446:476	Francisella live vaccine strain (LVS)	446:482	To pursue the possibility that the capsule of Francisella live vaccine strain (LVS) has a structurally unique lipid anchor, we have metabolically labeled Francisella with [14C]acetate to facilitate highly sensitive compositional analysis of capsule-associated lipids.
27326857	3	64	theme	A	913:913	arg1	A					913:913	free lipid A	902:913	free lipid A	902:913	Autoradiographic and immunologic analysis confirmed that this purified material was largely devoid of low molecular weight LPS and of the copious amounts of free lipid A that the Francisellae accumulate.
27326857	3	64	theme	A	913:913	arg1	amounts					891:897	the copious amounts	879:897	the copious amounts of free lipid A	879:913	Autoradiographic and immunologic analysis confirmed that this purified material was largely devoid of low molecular weight LPS and of the copious amounts of free lipid A that the Francisellae accumulate.
27326857	0	65	theme	Lipid	77:81	arg1	Molecule					90:97	a Lipid A-Like Molecule	75:97	a Lipid A-Like Molecule That Is Structurally Distinct from the More Abundant Free Lipid A. Francisella tularensis, the Gram-negative bacterium that causes tularemia, produces a high molecular weight capsule that is immunologically distinct from Francisella lipopolysaccharide but contains the same O-antigen tetrasaccharide	75:397	Evidence Suggesting That Francisella tularensis O-Antigen Capsule Contains a Lipid A-Like Molecule That Is Structurally Distinct from the More Abundant Free Lipid A. Francisella tularensis, the Gram-negative bacterium that causes tularemia, produces a high molecular weight capsule that is immunologically distinct from Francisella lipopolysaccharide but contains the same O-antigen tetrasaccharide.
27326857	1	66	theme	vaccine	463:469	arg1	LVS					479:481	LVS	479:481	LVS	479:481	To pursue the possibility that the capsule of Francisella live vaccine strain (LVS) has a structurally unique lipid anchor, we have metabolically labeled Francisella with [14C]acetate to facilitate highly sensitive compositional analysis of capsule-associated lipids.
27326857	1	66	theme	vaccine	463:469	arg1	strain					471:476	Francisella live vaccine strain	446:476	Francisella live vaccine strain (LVS)	446:482	To pursue the possibility that the capsule of Francisella live vaccine strain (LVS) has a structurally unique lipid anchor, we have metabolically labeled Francisella with [14C]acetate to facilitate highly sensitive compositional analysis of capsule-associated lipids.
27326857	0	67	theme	Francisella	320:330	arg1	lipopolysaccharide					332:349	Francisella lipopolysaccharide	320:349	Francisella lipopolysaccharide	320:349	Evidence Suggesting That Francisella tularensis O-Antigen Capsule Contains a Lipid A-Like Molecule That Is Structurally Distinct from the More Abundant Free Lipid A. Francisella tularensis, the Gram-negative bacterium that causes tularemia, produces a high molecular weight capsule that is immunologically distinct from Francisella lipopolysaccharide but contains the same O-antigen tetrasaccharide.
27326857	3	68	theme	free	902:905	arg1	A					913:913	free lipid A	902:913	free lipid A	902:913	Autoradiographic and immunologic analysis confirmed that this purified material was largely devoid of low molecular weight LPS and of the copious amounts of free lipid A that the Francisellae accumulate.
27326857	4	69	theme	Francisella	1026:1036	arg1	A					1044:1044	Francisella lipid A	1026:1044	Francisella lipid A	1026:1044	Chemical hydrolysis yielded [14C]-labeled free fatty acids characteristic of Francisella lipid A but with a different molar ratio of 3-OH C18:0 to 3-OH C16:0 and different composition of non-hydroxylated fatty acids (mainly C14:0 rather than C16:0) than that of free Francisella lipid A. Mild acid hydrolysis to induce selective cleavage of KDO-lipid A linkage yielded a [14C]-labeled product that partitioned during Bligh/Dyer extraction and migrated during thin-layer chromatography like lipid A.
27326857	6	70	theme	capsular	1844:1851	arg1	layer					1853:1857	the O-antigen capsular layer	1830:1857	the O-antigen capsular layer	1830:1857	The presence of a discrete lipid A-like molecule associated with capsule raises the possibility that Francisella selectively exploits lipid A structural heterogeneity to regulate synthesis, transport, and stable bacterial surface association of the O-antigen capsular layer.
27326857	1	71	theme	lipids	660:665	arg1	analysis					629:636	highly sensitive compositional analysis	598:636	highly sensitive compositional analysis of capsule-associated lipids	598:665	To pursue the possibility that the capsule of Francisella live vaccine strain (LVS) has a structurally unique lipid anchor, we have metabolically labeled Francisella with [14C]acetate to facilitate highly sensitive compositional analysis of capsule-associated lipids.
27326857	3	72	theme	weight	861:866	arg1	LPS					868:870	low molecular weight LPS	847:870	low molecular weight LPS	847:870	Autoradiographic and immunologic analysis confirmed that this purified material was largely devoid of low molecular weight LPS and of the copious amounts of free lipid A that the Francisellae accumulate.
27326857	5	73	theme	O-antigen	1480:1488	arg1	capsule					1490:1496	the O-antigen capsule	1476:1496	the O-antigen capsule of Francisella	1476:1511	These findings suggest that the O-antigen capsule of Francisella contains a covalently linked and structurally distinct lipid A species.
27326857	6	74	theme	lipid	1612:1616	arg1	molecule					1625:1632	a discrete lipid A-like molecule	1601:1632	a discrete lipid A-like molecule associated with capsule	1601:1656	The presence of a discrete lipid A-like molecule associated with capsule raises the possibility that Francisella selectively exploits lipid A structural heterogeneity to regulate synthesis, transport, and stable bacterial surface association of the O-antigen capsular layer.
27326857	4	75	theme	A	1044:1044	arg1	characteristic					1008:1021	characteristic	1008:1021	characteristic	1008:1021	Chemical hydrolysis yielded [14C]-labeled free fatty acids characteristic of Francisella lipid A but with a different molar ratio of 3-OH C18:0 to 3-OH C16:0 and different composition of non-hydroxylated fatty acids (mainly C14:0 rather than C16:0) than that of free Francisella lipid A. Mild acid hydrolysis to induce selective cleavage of KDO-lipid A linkage yielded a [14C]-labeled product that partitioned during Bligh/Dyer extraction and migrated during thin-layer chromatography like lipid A.
27326857	2	76	theme	independent	696:706	arg1	methods					708:714	two independent methods	692:714	two independent methods	692:714	Capsule was purified by two independent methods and yielded similar results.
27326857	3	77	theme	amounts	891:897	arg1	devoid					837:842	devoid	837:842	devoid	837:842	Autoradiographic and immunologic analysis confirmed that this purified material was largely devoid of low molecular weight LPS and of the copious amounts of free lipid A that the Francisellae accumulate.
27326857	6	78	theme	lipid	1719:1723	arg1	A					1725:1725	lipid A	1719:1725	lipid A	1719:1725	The presence of a discrete lipid A-like molecule associated with capsule raises the possibility that Francisella selectively exploits lipid A structural heterogeneity to regulate synthesis, transport, and stable bacterial surface association of the O-antigen capsular layer.
27326857	4	79	theme	fatty	996:1000	arg1	acids					1002:1006	[14C]-labeled free fatty acids	977:1006	[14C]-labeled free fatty acids characteristic of Francisella lipid A but with a different molar ratio of 3-OH C18:0 to 3-OH C16:0 and different composition of non-hydroxylated fatty acids (mainly C14:0 rather than C16:0)	977:1196	Chemical hydrolysis yielded [14C]-labeled free fatty acids characteristic of Francisella lipid A but with a different molar ratio of 3-OH C18:0 to 3-OH C16:0 and different composition of non-hydroxylated fatty acids (mainly C14:0 rather than C16:0) than that of free Francisella lipid A. Mild acid hydrolysis to induce selective cleavage of KDO-lipid A linkage yielded a [14C]-labeled product that partitioned during Bligh/Dyer extraction and migrated during thin-layer chromatography like lipid A.
27326857	5	80	theme	lipid	1568:1572	arg1	species					1576:1582	a covalently linked and structurally distinct lipid A species	1522:1582	a covalently linked and structurally distinct lipid A species	1522:1582	These findings suggest that the O-antigen capsule of Francisella contains a covalently linked and structurally distinct lipid A species.
27326857	5	81	contain	contains	1513:1520	arg1	capsule					1490:1496	the O-antigen capsule	1476:1496	the O-antigen capsule of Francisella	1476:1511	These findings suggest that the O-antigen capsule of Francisella contains a covalently linked and structurally distinct lipid A species.
27326857	5	81	contain	contains	1513:1520	arg2	species					1576:1582	a covalently linked and structurally distinct lipid A species	1522:1582	a covalently linked and structurally distinct lipid A species	1522:1582	These findings suggest that the O-antigen capsule of Francisella contains a covalently linked and structurally distinct lipid A species.
27326857	1	82	contain	have	527:530	arg2	Francisella					554:564	metabolically labeled Francisella	532:564	metabolically labeled Francisella with [14C]acetate to facilitate highly sensitive compositional analysis of capsule-associated lipids	532:665	To pursue the possibility that the capsule of Francisella live vaccine strain (LVS) has a structurally unique lipid anchor, we have metabolically labeled Francisella with [14C]acetate to facilitate highly sensitive compositional analysis of capsule-associated lipids.
27326857	1	82	contain	have	527:530	arg1	we					524:525	we	524:525	we	524:525	To pursue the possibility that the capsule of Francisella live vaccine strain (LVS) has a structurally unique lipid anchor, we have metabolically labeled Francisella with [14C]acetate to facilitate highly sensitive compositional analysis of capsule-associated lipids.
27326857	6	83	theme	bacterial	1797:1805	arg1	association					1815:1825	stable bacterial surface association	1790:1825	stable bacterial surface association	1790:1825	The presence of a discrete lipid A-like molecule associated with capsule raises the possibility that Francisella selectively exploits lipid A structural heterogeneity to regulate synthesis, transport, and stable bacterial surface association of the O-antigen capsular layer.
27326857	4	84	theme	acid	1242:1245	arg1	hydrolysis					1247:1256	Mild acid hydrolysis	1237:1256	Mild acid hydrolysis to induce selective cleavage of KDO-lipid A linkage yielded a [14C]-labeled product that partitioned during Bligh/Dyer extraction and migrated during thin-layer chromatography like lipid A	1237:1445	Chemical hydrolysis yielded [14C]-labeled free fatty acids characteristic of Francisella lipid A but with a different molar ratio of 3-OH C18:0 to 3-OH C16:0 and different composition of non-hydroxylated fatty acids (mainly C14:0 rather than C16:0) than that of free Francisella lipid A. Mild acid hydrolysis to induce selective cleavage of KDO-lipid A linkage yielded a [14C]-labeled product that partitioned during Bligh/Dyer extraction and migrated during thin-layer chromatography like lipid A.
27326857	0	85	theme	molecular	257:265	arg1	weight					267:272	a high molecular weight	250:272	a high molecular weight capsule that is immunologically distinct from Francisella lipopolysaccharide	250:349	Evidence Suggesting That Francisella tularensis O-Antigen Capsule Contains a Lipid A-Like Molecule That Is Structurally Distinct from the More Abundant Free Lipid A. Francisella tularensis, the Gram-negative bacterium that causes tularemia, produces a high molecular weight capsule that is immunologically distinct from Francisella lipopolysaccharide but contains the same O-antigen tetrasaccharide.
27326857	0	86	dep	Molecule	90:97	arg1	Distinct					120:127	Distinct	120:127	Distinct	120:127	Evidence Suggesting That Francisella tularensis O-Antigen Capsule Contains a Lipid A-Like Molecule That Is Structurally Distinct from the More Abundant Free Lipid A. Francisella tularensis, the Gram-negative bacterium that causes tularemia, produces a high molecular weight capsule that is immunologically distinct from Francisella lipopolysaccharide but contains the same O-antigen tetrasaccharide.
27326857	5	87	theme	linked	1535:1540	arg1	species					1576:1582	a covalently linked and structurally distinct lipid A species	1522:1582	a covalently linked and structurally distinct lipid A species	1522:1582	These findings suggest that the O-antigen capsule of Francisella contains a covalently linked and structurally distinct lipid A species.
27326857	0	88	theme	Francisella	25:35	arg1	tularensis					37:46	Francisella tularensis	25:46	Francisella tularensis O-Antigen Capsule	25:64	Evidence Suggesting That Francisella tularensis O-Antigen Capsule Contains a Lipid A-Like Molecule That Is Structurally Distinct from the More Abundant Free Lipid A. Francisella tularensis, the Gram-negative bacterium that causes tularemia, produces a high molecular weight capsule that is immunologically distinct from Francisella lipopolysaccharide but contains the same O-antigen tetrasaccharide.
27326857	4	89	theme	3-OH	1082:1085	arg1	C18:0					1087:1091	3-OH C18:0	1082:1091	3-OH C18:0	1082:1091	Chemical hydrolysis yielded [14C]-labeled free fatty acids characteristic of Francisella lipid A but with a different molar ratio of 3-OH C18:0 to 3-OH C16:0 and different composition of non-hydroxylated fatty acids (mainly C14:0 rather than C16:0) than that of free Francisella lipid A. Mild acid hydrolysis to induce selective cleavage of KDO-lipid A linkage yielded a [14C]-labeled product that partitioned during Bligh/Dyer extraction and migrated during thin-layer chromatography like lipid A.
27326857	0	90	theme	O-Antigen	48:56	arg1	Capsule					58:64	Francisella tularensis O-Antigen Capsule	25:64	Francisella tularensis O-Antigen Capsule	25:64	Evidence Suggesting That Francisella tularensis O-Antigen Capsule Contains a Lipid A-Like Molecule That Is Structurally Distinct from the More Abundant Free Lipid A. Francisella tularensis, the Gram-negative bacterium that causes tularemia, produces a high molecular weight capsule that is immunologically distinct from Francisella lipopolysaccharide but contains the same O-antigen tetrasaccharide.
27326857	4	91	theme	-labeled	1325:1332	arg1	product					1334:1340	a [14C]-labeled product	1318:1340	a [14C]-labeled product that partitioned during Bligh/Dyer extraction and migrated during thin-layer chromatography like lipid A	1318:1445	Chemical hydrolysis yielded [14C]-labeled free fatty acids characteristic of Francisella lipid A but with a different molar ratio of 3-OH C18:0 to 3-OH C16:0 and different composition of non-hydroxylated fatty acids (mainly C14:0 rather than C16:0) than that of free Francisella lipid A. Mild acid hydrolysis to induce selective cleavage of KDO-lipid A linkage yielded a [14C]-labeled product that partitioned during Bligh/Dyer extraction and migrated during thin-layer chromatography like lipid A.
27326857	1	92	theme	lipid	510:514	arg1	anchor					516:521	a structurally unique lipid anchor	488:521	a structurally unique lipid anchor	488:521	To pursue the possibility that the capsule of Francisella live vaccine strain (LVS) has a structurally unique lipid anchor, we have metabolically labeled Francisella with [14C]acetate to facilitate highly sensitive compositional analysis of capsule-associated lipids.
27326857	4	93	theme	characteristic	1008:1021	arg1	acids					1002:1006	[14C]-labeled free fatty acids	977:1006	[14C]-labeled free fatty acids characteristic of Francisella lipid A but with a different molar ratio of 3-OH C18:0 to 3-OH C16:0 and different composition of non-hydroxylated fatty acids (mainly C14:0 rather than C16:0)	977:1196	Chemical hydrolysis yielded [14C]-labeled free fatty acids characteristic of Francisella lipid A but with a different molar ratio of 3-OH C18:0 to 3-OH C16:0 and different composition of non-hydroxylated fatty acids (mainly C14:0 rather than C16:0) than that of free Francisella lipid A. Mild acid hydrolysis to induce selective cleavage of KDO-lipid A linkage yielded a [14C]-labeled product that partitioned during Bligh/Dyer extraction and migrated during thin-layer chromatography like lipid A.
27326857	4	94	theme	different	1057:1065	arg1	ratio					1073:1077	a different molar ratio	1055:1077	a different molar ratio of 3-OH C18:0 to 3-OH C16:0 and different composition of non-hydroxylated fatty acids (mainly C14:0 rather than C16:0)	1055:1196	Chemical hydrolysis yielded [14C]-labeled free fatty acids characteristic of Francisella lipid A but with a different molar ratio of 3-OH C18:0 to 3-OH C16:0 and different composition of non-hydroxylated fatty acids (mainly C14:0 rather than C16:0) than that of free Francisella lipid A. Mild acid hydrolysis to induce selective cleavage of KDO-lipid A linkage yielded a [14C]-labeled product that partitioned during Bligh/Dyer extraction and migrated during thin-layer chromatography like lipid A.
27326857	4	95	theme	A	1300:1300	arg1	linkage					1302:1308	KDO-lipid A linkage	1290:1308	KDO-lipid A linkage yielded a [14C]-labeled product that partitioned during Bligh/Dyer extraction and migrated during thin-layer chromatography like lipid A	1290:1445	Chemical hydrolysis yielded [14C]-labeled free fatty acids characteristic of Francisella lipid A but with a different molar ratio of 3-OH C18:0 to 3-OH C16:0 and different composition of non-hydroxylated fatty acids (mainly C14:0 rather than C16:0) than that of free Francisella lipid A. Mild acid hydrolysis to induce selective cleavage of KDO-lipid A linkage yielded a [14C]-labeled product that partitioned during Bligh/Dyer extraction and migrated during thin-layer chromatography like lipid A.
27326857	2	96	theme	similar	728:734	arg1	results					736:742	similar results	728:742	similar results	728:742	Capsule was purified by two independent methods and yielded similar results.
27326857	4	97	theme	different	1111:1119	arg1	composition					1121:1131	different composition	1111:1131	different composition	1111:1131	Chemical hydrolysis yielded [14C]-labeled free fatty acids characteristic of Francisella lipid A but with a different molar ratio of 3-OH C18:0 to 3-OH C16:0 and different composition of non-hydroxylated fatty acids (mainly C14:0 rather than C16:0) than that of free Francisella lipid A. Mild acid hydrolysis to induce selective cleavage of KDO-lipid A linkage yielded a [14C]-labeled product that partitioned during Bligh/Dyer extraction and migrated during thin-layer chromatography like lipid A.
27326857	4	98	with	characteristic	1008:1021	arg1	ratio					1073:1077	a different molar ratio	1055:1077	a different molar ratio of 3-OH C18:0 to 3-OH C16:0 and different composition of non-hydroxylated fatty acids (mainly C14:0 rather than C16:0)	1055:1196	Chemical hydrolysis yielded [14C]-labeled free fatty acids characteristic of Francisella lipid A but with a different molar ratio of 3-OH C18:0 to 3-OH C16:0 and different composition of non-hydroxylated fatty acids (mainly C14:0 rather than C16:0) than that of free Francisella lipid A. Mild acid hydrolysis to induce selective cleavage of KDO-lipid A linkage yielded a [14C]-labeled product that partitioned during Bligh/Dyer extraction and migrated during thin-layer chromatography like lipid A.
27326857	0	99	contain	contains	355:362	arg1	tularensis					178:187	the More Abundant Free Lipid A. Francisella tularensis	134:187	the More Abundant Free Lipid A. Francisella tularensis	134:187	Evidence Suggesting That Francisella tularensis O-Antigen Capsule Contains a Lipid A-Like Molecule That Is Structurally Distinct from the More Abundant Free Lipid A. Francisella tularensis, the Gram-negative bacterium that causes tularemia, produces a high molecular weight capsule that is immunologically distinct from Francisella lipopolysaccharide but contains the same O-antigen tetrasaccharide.
27326857	0	99	contain	contains	355:362	arg2	tetrasaccharide					383:397	the same O-antigen tetrasaccharide	364:397	the same O-antigen tetrasaccharide	364:397	Evidence Suggesting That Francisella tularensis O-Antigen Capsule Contains a Lipid A-Like Molecule That Is Structurally Distinct from the More Abundant Free Lipid A. Francisella tularensis, the Gram-negative bacterium that causes tularemia, produces a high molecular weight capsule that is immunologically distinct from Francisella lipopolysaccharide but contains the same O-antigen tetrasaccharide.
27326857	0	99	contain	contains	355:362	arg1	bacterium					208:216	the Gram-negative bacterium	190:216	the Gram-negative bacterium that causes tularemia, produces a high molecular weight capsule that is immunologically distinct from Francisella lipopolysaccharide but contains the same O-antigen tetrasaccharide	190:397	Evidence Suggesting That Francisella tularensis O-Antigen Capsule Contains a Lipid A-Like Molecule That Is Structurally Distinct from the More Abundant Free Lipid A. Francisella tularensis, the Gram-negative bacterium that causes tularemia, produces a high molecular weight capsule that is immunologically distinct from Francisella lipopolysaccharide but contains the same O-antigen tetrasaccharide.
27326857	3	100	theme	Autoradiographic	745:760	arg1	analysis					778:785	Autoradiographic and immunologic analysis	745:785	Autoradiographic and immunologic analysis	745:785	Autoradiographic and immunologic analysis confirmed that this purified material was largely devoid of low molecular weight LPS and of the copious amounts of free lipid A that the Francisellae accumulate.
27326857	1	101	theme	labeled	546:552	arg1	Francisella					554:564	metabolically labeled Francisella	532:564	metabolically labeled Francisella with [14C]acetate to facilitate highly sensitive compositional analysis of capsule-associated lipids	532:665	To pursue the possibility that the capsule of Francisella live vaccine strain (LVS) has a structurally unique lipid anchor, we have metabolically labeled Francisella with [14C]acetate to facilitate highly sensitive compositional analysis of capsule-associated lipids.
29174356	0	0	theme	sulfates	93:100	arg1	purification					12:23	purification	12:23	purification	12:23	Separation, purification, structures and anticoagulant activities of fucosylated chondroitin sulfates from Holothuria scabra.
29174356	0	0	theme	sulfates	93:100	arg1	Separation					0:9	Separation	0:9	Separation	0:9	Separation, purification, structures and anticoagulant activities of fucosylated chondroitin sulfates from Holothuria scabra.
29174356	0	0	theme	sulfates	93:100	arg1	structures					26:35	structures	26:35	structures	26:35	Separation, purification, structures and anticoagulant activities of fucosylated chondroitin sulfates from Holothuria scabra.
29174356	0	0	theme	sulfates	93:100	arg1	activities					55:64	anticoagulant activities	41:64	anticoagulant activities	41:64	Separation, purification, structures and anticoagulant activities of fucosylated chondroitin sulfates from Holothuria scabra.
29174356	4	1	theme	molar	1294:1298	arg1	ratio					1300:1304	the molar ratio	1290:1304	the molar ratio of 1:1.72:2.34:3.29	1290:1324	The backbone →4)GlcUAβ(1 → 3)GalNAcβ(1→ and sulfated fucose branches were composed of the β-d-glucuronic acid, N-acetyl-β-d- galactosamine, α-l-fucose and sulfate groups by the molar ratio of 1:1.72:2.34:3.29.
29174356	1	2	theme	fucosylated	147:157	arg1	HsG					180:182	HsG	180:182	HsG	180:182	In this study, a new fucosylated chondroitin sulfate (HsG) with an average molecular weight of 69.1 kDa was isolated from sea cucumber Holothuria scabra.
29174356	1	2	theme	fucosylated	147:157	arg1	sulfate					171:177	a new fucosylated chondroitin sulfate	141:177	a new fucosylated chondroitin sulfate (HsG) with an average molecular weight of 69.1 kDa	141:228	In this study, a new fucosylated chondroitin sulfate (HsG) with an average molecular weight of 69.1 kDa was isolated from sea cucumber Holothuria scabra.
29174356	3	3	theme	acid	916:919	arg1	position					889:896	the O-3 position	881:896	the O-3 position of β-d-glucuronic acid	881:919	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	3	4	attach	linked	1075:1080	arg2	residues					858:865	the fucopyranosyl residues	840:865	the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid	840:919	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	3	4	attach	linked	1075:1080	arg2	%					926:926	13.0%	922:926	13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety	922:1024	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	3	4	attach	linked	1075:1080	arg2	residues					754:761	the fucopyranosyl residues	736:761	the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety	736:828	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	3	4	attach	linked	1075:1080	arg2	residues					949:956	the fucopyranosyl residues	931:956	the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety	931:1024	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	3	4	attach	linked	1075:1080	arg2	%					835:835	21.2%	831:835	21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid	831:919	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	3	4	attach	linked	1075:1080	arg2	residues					1058:1065	the fucopyranosyl residues	1040:1065	the fucopyranosyl residues	1040:1065	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	3	4	attach	linked	1075:1080	arg1	groups					1093:1098	sulfate groups	1085:1098	sulfate groups on the backbone	1085:1114	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	3	4	attach	linked	1075:1080	arg2	%					1035:1035	10.1%	1031:1035	10.1% of the fucopyranosyl residues	1031:1065	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	3	4	attach	linked	1075:1080	arg2	%					731:731	55.7%	727:731	55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety	727:828	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	0	5	theme	chondroitin	81:91	arg1	sulfates					93:100	fucosylated chondroitin sulfates	69:100	fucosylated chondroitin sulfates	69:100	Separation, purification, structures and anticoagulant activities of fucosylated chondroitin sulfates from Holothuria scabra.
29174356	3	6	dep	residues	858:865	arg1	linked					871:876	linked	871:876	are linked to the O-3 position of β-d-glucuronic acid	867:919	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	1	7	theme	chondroitin	159:169	arg1	HsG					180:182	HsG	180:182	HsG	180:182	In this study, a new fucosylated chondroitin sulfate (HsG) with an average molecular weight of 69.1 kDa was isolated from sea cucumber Holothuria scabra.
29174356	1	7	theme	chondroitin	159:169	arg1	sulfate					171:177	a new fucosylated chondroitin sulfate	141:177	a new fucosylated chondroitin sulfate (HsG) with an average molecular weight of 69.1 kDa	141:228	In this study, a new fucosylated chondroitin sulfate (HsG) with an average molecular weight of 69.1 kDa was isolated from sea cucumber Holothuria scabra.
29174356	4	8	theme	fucose	1170:1175	arg1	branches					1177:1184	sulfated fucose branches	1161:1184	sulfated fucose branches	1161:1184	The backbone →4)GlcUAβ(1 → 3)GalNAcβ(1→ and sulfated fucose branches were composed of the β-d-glucuronic acid, N-acetyl-β-d- galactosamine, α-l-fucose and sulfate groups by the molar ratio of 1:1.72:2.34:3.29.
29174356	3	9	theme	residues	1058:1065	arg1	%					835:835	21.2%	831:835	21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid	831:919	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	3	9	theme	residues	1058:1065	arg1	residues					754:761	the fucopyranosyl residues	736:761	the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety	736:828	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	3	9	theme	residues	1058:1065	arg1	residues					1058:1065	the fucopyranosyl residues	1040:1065	the fucopyranosyl residues	1040:1065	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	3	9	theme	residues	1058:1065	arg1	residues					949:956	the fucopyranosyl residues	931:956	the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety	931:1024	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	3	9	theme	residues	1058:1065	arg1	%					731:731	55.7%	727:731	55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety	727:828	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	3	9	theme	residues	1058:1065	arg1	%					1035:1035	10.1%	1031:1035	10.1% of the fucopyranosyl residues	1031:1065	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	3	9	theme	residues	1058:1065	arg1	%					926:926	13.0%	922:926	13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety	922:1024	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	3	9	theme	residues	1058:1065	arg1	residues					858:865	the fucopyranosyl residues	840:865	the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid	840:919	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	4	10	theme	acid	1222:1225	arg1	groups					1280:1285	the β-d-glucuronic acid, N-acetyl-β-d- galactosamine, α-l-fucose and sulfate groups	1203:1285	the β-d-glucuronic acid, N-acetyl-β-d- galactosamine, α-l-fucose and sulfate groups	1203:1285	The backbone →4)GlcUAβ(1 → 3)GalNAcβ(1→ and sulfated fucose branches were composed of the β-d-glucuronic acid, N-acetyl-β-d- galactosamine, α-l-fucose and sulfate groups by the molar ratio of 1:1.72:2.34:3.29.
29174356	1	11	theme	sea	248:250	arg1	cucumber					252:259	sea cucumber	248:259	sea cucumber	248:259	In this study, a new fucosylated chondroitin sulfate (HsG) with an average molecular weight of 69.1 kDa was isolated from sea cucumber Holothuria scabra.
29174356	0	12	gly	fucosylated	69:79	arg1	sulfates					93:100	fucosylated chondroitin sulfates	69:100	fucosylated chondroitin sulfates	69:100	Separation, purification, structures and anticoagulant activities of fucosylated chondroitin sulfates from Holothuria scabra.
29174356	3	13	theme	moiety	1019:1024	arg1	positions					980:988	the O-4 positions	972:988	the O-4 positions of the N-acetylgalactosamine moiety	972:1024	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	2	14	theme	composition	348:358	arg1	analysis					360:367	monosaccharide composition analysis	333:367	monosaccharide composition analysis	333:367	We investigated the structure of the HsG by adopting monosaccharide composition analysis, disaccharide composition analysis, IR,1H and13C NMR spectra and methylation analysis.
29174356	4	15	theme	→4	1130:1131	arg1	1→					1154:1155	The backbone →4)GlcUAβ(1 → 3)GalNAcβ(1→	1117:1155	The backbone →4)GlcUAβ(1 → 3)GalNAcβ(1→	1117:1155	The backbone →4)GlcUAβ(1 → 3)GalNAcβ(1→ and sulfated fucose branches were composed of the β-d-glucuronic acid, N-acetyl-β-d- galactosamine, α-l-fucose and sulfate groups by the molar ratio of 1:1.72:2.34:3.29.
29174356	3	16	theme	fucopyranosyl	844:856	arg1	residues					858:865	the fucopyranosyl residues	840:865	the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid	840:919	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	4	17	theme	β-d-glucuronic	1207:1220	arg1	acid					1222:1225	the β-d-glucuronic acid	1203:1225	the β-d-glucuronic acid	1203:1225	The backbone →4)GlcUAβ(1 → 3)GalNAcβ(1→ and sulfated fucose branches were composed of the β-d-glucuronic acid, N-acetyl-β-d- galactosamine, α-l-fucose and sulfate groups by the molar ratio of 1:1.72:2.34:3.29.
29174356	3	18	theme	disaccharides	568:580	arg1	polysaccharides					520:534	desulfated/carboxyl-reduced polysaccharides	492:534	desulfated/carboxyl-reduced polysaccharides	492:534	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	3	18	theme	disaccharides	568:580	arg1	analysis					544:551	the analysis	540:551	the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides	540:647	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	4	19	theme	backbone	1121:1128	arg1	1→					1154:1155	The backbone →4)GlcUAβ(1 → 3)GalNAcβ(1→	1117:1155	The backbone →4)GlcUAβ(1 → 3)GalNAcβ(1→	1117:1155	The backbone →4)GlcUAβ(1 → 3)GalNAcβ(1→ and sulfated fucose branches were composed of the β-d-glucuronic acid, N-acetyl-β-d- galactosamine, α-l-fucose and sulfate groups by the molar ratio of 1:1.72:2.34:3.29.
29174356	3	20	dep	wherein	719:725	arg1	linked					1075:1080	linked	1075:1080	are not linked to sulfate groups on the backbone	1067:1114	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	3	21	theme	fucopyranosyl	740:752	arg1	residues					754:761	the fucopyranosyl residues	736:761	the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety	736:828	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	3	22	theme	residues	858:865	arg1	%					835:835	21.2%	831:835	21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid	831:919	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	3	22	theme	residues	858:865	arg1	residues					754:761	the fucopyranosyl residues	736:761	the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety	736:828	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	3	22	theme	residues	858:865	arg1	residues					1058:1065	the fucopyranosyl residues	1040:1065	the fucopyranosyl residues	1040:1065	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	3	22	theme	residues	858:865	arg1	residues					949:956	the fucopyranosyl residues	931:956	the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety	931:1024	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	3	22	theme	residues	858:865	arg1	%					731:731	55.7%	727:731	55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety	727:828	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	3	22	theme	residues	858:865	arg1	%					1035:1035	10.1%	1031:1035	10.1% of the fucopyranosyl residues	1031:1065	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	3	22	theme	residues	858:865	arg1	%					926:926	13.0%	922:926	13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety	922:1024	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	3	22	theme	residues	858:865	arg1	residues					858:865	the fucopyranosyl residues	840:865	the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid	840:919	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	4	23	theme	GlcUAβ	1133:1138	arg1	1→					1154:1155	The backbone →4)GlcUAβ(1 → 3)GalNAcβ(1→	1117:1155	The backbone →4)GlcUAβ(1 → 3)GalNAcβ(1→	1117:1155	The backbone →4)GlcUAβ(1 → 3)GalNAcβ(1→ and sulfated fucose branches were composed of the β-d-glucuronic acid, N-acetyl-β-d- galactosamine, α-l-fucose and sulfate groups by the molar ratio of 1:1.72:2.34:3.29.
29174356	4	24	theme	GalNAcβ	1146:1152	arg1	1→					1154:1155	The backbone →4)GlcUAβ(1 → 3)GalNAcβ(1→	1117:1155	The backbone →4)GlcUAβ(1 → 3)GalNAcβ(1→	1117:1155	The backbone →4)GlcUAβ(1 → 3)GalNAcβ(1→ and sulfated fucose branches were composed of the β-d-glucuronic acid, N-acetyl-β-d- galactosamine, α-l-fucose and sulfate groups by the molar ratio of 1:1.72:2.34:3.29.
29174356	3	25	theme	residues	754:761	arg1	%					835:835	21.2%	831:835	21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid	831:919	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	3	25	theme	residues	754:761	arg1	residues					754:761	the fucopyranosyl residues	736:761	the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety	736:828	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	3	25	theme	residues	754:761	arg1	residues					1058:1065	the fucopyranosyl residues	1040:1065	the fucopyranosyl residues	1040:1065	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	3	25	theme	residues	754:761	arg1	residues					949:956	the fucopyranosyl residues	931:956	the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety	931:1024	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	3	25	theme	residues	754:761	arg1	%					731:731	55.7%	727:731	55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety	727:828	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	3	25	theme	residues	754:761	arg1	%					1035:1035	10.1%	1031:1035	10.1% of the fucopyranosyl residues	1031:1065	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	3	25	theme	residues	754:761	arg1	%					926:926	13.0%	922:926	13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety	922:1024	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	3	25	theme	residues	754:761	arg1	residues					858:865	the fucopyranosyl residues	840:865	the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid	840:919	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	3	26	theme	fucopyranosyl	1044:1056	arg1	residues					1058:1065	the fucopyranosyl residues	1040:1065	the fucopyranosyl residues	1040:1065	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	2	27	theme	composition	383:393	arg1	analysis					395:402	disaccharide composition analysis	370:402	disaccharide composition analysis	370:402	We investigated the structure of the HsG by adopting monosaccharide composition analysis, disaccharide composition analysis, IR,1H and13C NMR spectra and methylation analysis.
29174356	3	28	theme	O-6	781:783	arg1	position					785:792	the O-6 position	777:792	the O-6 position of the N-acetylgalactosamine moiety	777:828	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	3	29	theme	sulfate	1085:1091	arg1	groups					1093:1098	sulfate groups	1085:1098	sulfate groups on the backbone	1085:1114	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	3	30	theme	defucosed	623:631	arg1	polysaccharides					633:647	the defucosed polysaccharides	619:647	the defucosed polysaccharides	619:647	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	3	31	theme	residues	949:956	arg1	%					835:835	21.2%	831:835	21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid	831:919	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	3	31	theme	residues	949:956	arg1	residues					754:761	the fucopyranosyl residues	736:761	the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety	736:828	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	3	31	theme	residues	949:956	arg1	residues					1058:1065	the fucopyranosyl residues	1040:1065	the fucopyranosyl residues	1040:1065	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	3	31	theme	residues	949:956	arg1	residues					949:956	the fucopyranosyl residues	931:956	the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety	931:1024	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	3	31	theme	residues	949:956	arg1	%					731:731	55.7%	727:731	55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety	727:828	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	3	31	theme	residues	949:956	arg1	%					1035:1035	10.1%	1031:1035	10.1% of the fucopyranosyl residues	1031:1065	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	3	31	theme	residues	949:956	arg1	%					926:926	13.0%	922:926	13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety	922:1024	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	3	31	theme	residues	949:956	arg1	residues					858:865	the fucopyranosyl residues	840:865	the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid	840:919	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	2	32	theme	disaccharide	370:381	arg1	analysis					395:402	disaccharide composition analysis	370:402	disaccharide composition analysis	370:402	We investigated the structure of the HsG by adopting monosaccharide composition analysis, disaccharide composition analysis, IR,1H and13C NMR spectra and methylation analysis.
29174356	2	33	theme	methylation	434:444	arg1	analysis					446:453	methylation analysis	434:453	methylation analysis	434:453	We investigated the structure of the HsG by adopting monosaccharide composition analysis, disaccharide composition analysis, IR,1H and13C NMR spectra and methylation analysis.
29174356	3	34	theme	methylation	469:479	arg1	results					481:487	methylation results	469:487	methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides	469:647	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	3	35	theme	unsaturated	556:566	arg1	disaccharides					568:580	unsaturated disaccharides	556:580	unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides	556:647	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	3	36	from	groups	1093:1098	arg1	backbone					1107:1114	the backbone	1103:1114	the backbone	1103:1114	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	4	37	theme	sulfated	1161:1168	arg1	branches					1177:1184	sulfated fucose branches	1161:1184	sulfated fucose branches	1161:1184	The backbone →4)GlcUAβ(1 → 3)GalNAcβ(1→ and sulfated fucose branches were composed of the β-d-glucuronic acid, N-acetyl-β-d- galactosamine, α-l-fucose and sulfate groups by the molar ratio of 1:1.72:2.34:3.29.
29174356	2	38	theme	monosaccharide	333:346	arg1	analysis					360:367	monosaccharide composition analysis	333:367	monosaccharide composition analysis	333:367	We investigated the structure of the HsG by adopting monosaccharide composition analysis, disaccharide composition analysis, IR,1H and13C NMR spectra and methylation analysis.
29174356	2	39	theme	NMR	418:420	arg1	spectra					422:428	IR,1H and13C NMR spectra	405:428	IR,1H and13C NMR spectra	405:428	We investigated the structure of the HsG by adopting monosaccharide composition analysis, disaccharide composition analysis, IR,1H and13C NMR spectra and methylation analysis.
29174356	1	40	theme	average	193:199	arg1	weight					211:216	an average molecular weight	190:216	an average molecular weight of 69.1 kDa	190:228	In this study, a new fucosylated chondroitin sulfate (HsG) with an average molecular weight of 69.1 kDa was isolated from sea cucumber Holothuria scabra.
29174356	4	41	theme	α-l-fucose	1257:1266	arg1	groups					1280:1285	the β-d-glucuronic acid, N-acetyl-β-d- galactosamine, α-l-fucose and sulfate groups	1203:1285	the β-d-glucuronic acid, N-acetyl-β-d- galactosamine, α-l-fucose and sulfate groups	1203:1285	The backbone →4)GlcUAβ(1 → 3)GalNAcβ(1→ and sulfated fucose branches were composed of the β-d-glucuronic acid, N-acetyl-β-d- galactosamine, α-l-fucose and sulfate groups by the molar ratio of 1:1.72:2.34:3.29.
29174356	3	42	theme	fucopyranosyl	935:947	arg1	residues					949:956	the fucopyranosyl residues	931:956	the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety	931:1024	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	2	43	theme	and13C	411:416	arg1	spectra					422:428	IR,1H and13C NMR spectra	405:428	IR,1H and13C NMR spectra	405:428	We investigated the structure of the HsG by adopting monosaccharide composition analysis, disaccharide composition analysis, IR,1H and13C NMR spectra and methylation analysis.
29174356	3	44	theme	N-acetylgalactosamine	801:821	arg1	moiety					823:828	the N-acetylgalactosamine moiety	797:828	the N-acetylgalactosamine moiety	797:828	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	5	45	theme	HsG	1363:1365	arg1	activities					1345:1354	The anticoagulant activities	1327:1354	The anticoagulant activities of the HsG	1327:1365	The anticoagulant activities of the HsG was evaluated and compared with heparin.
29174356	1	46	theme	molecular	201:209	arg1	weight					211:216	an average molecular weight	190:216	an average molecular weight of 69.1 kDa	190:228	In this study, a new fucosylated chondroitin sulfate (HsG) with an average molecular weight of 69.1 kDa was isolated from sea cucumber Holothuria scabra.
29174356	3	47	theme	moiety	823:828	arg1	position					785:792	the O-6 position	777:792	the O-6 position of the N-acetylgalactosamine moiety	777:828	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	4	48	theme	sulfate	1272:1278	arg1	groups					1280:1285	the β-d-glucuronic acid, N-acetyl-β-d- galactosamine, α-l-fucose and sulfate groups	1203:1285	the β-d-glucuronic acid, N-acetyl-β-d- galactosamine, α-l-fucose and sulfate groups	1203:1285	The backbone →4)GlcUAβ(1 → 3)GalNAcβ(1→ and sulfated fucose branches were composed of the β-d-glucuronic acid, N-acetyl-β-d- galactosamine, α-l-fucose and sulfate groups by the molar ratio of 1:1.72:2.34:3.29.
29174356	3	49	theme	polysaccharides	633:647	arg1	enzymolysis					604:614	the enzymolysis	600:614	the enzymolysis of the defucosed polysaccharides	600:647	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	3	50	theme	O-4	976:978	arg1	positions					980:988	the O-4 positions	972:988	the O-4 positions of the N-acetylgalactosamine moiety	972:1024	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	3	51	theme	polysaccharides	520:534	arg1	results					481:487	methylation results	469:487	methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides	469:647	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	0	52	theme	anticoagulant	41:53	arg1	activities					55:64	anticoagulant activities	41:64	anticoagulant activities	41:64	Separation, purification, structures and anticoagulant activities of fucosylated chondroitin sulfates from Holothuria scabra.
29174356	4	53	theme	N-acetyl-β-d-	1228:1240	arg1	galactosamine					1242:1254	N-acetyl-β-d- galactosamine	1228:1254	N-acetyl-β-d- galactosamine	1228:1254	The backbone →4)GlcUAβ(1 → 3)GalNAcβ(1→ and sulfated fucose branches were composed of the β-d-glucuronic acid, N-acetyl-β-d- galactosamine, α-l-fucose and sulfate groups by the molar ratio of 1:1.72:2.34:3.29.
29174356	3	54	theme	analysis	544:551	arg1	results					481:487	methylation results	469:487	methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides	469:647	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	6	55	theme	thromboplastin	1475:1488	arg1	time					1490:1493	the activated partial thromboplastin time	1453:1493	the activated partial thromboplastin time	1453:1493	The result showed that the HsG could prolong the activated partial thromboplastin time.
29174356	1	56	with	sulfate	171:177	arg1	weight					211:216	an average molecular weight	190:216	an average molecular weight of 69.1 kDa	190:228	In this study, a new fucosylated chondroitin sulfate (HsG) with an average molecular weight of 69.1 kDa was isolated from sea cucumber Holothuria scabra.
29174356	0	57	from	scabra	118:123	arg1	purification					12:23	purification	12:23	purification	12:23	Separation, purification, structures and anticoagulant activities of fucosylated chondroitin sulfates from Holothuria scabra.
29174356	0	57	from	scabra	118:123	arg1	Separation					0:9	Separation	0:9	Separation	0:9	Separation, purification, structures and anticoagulant activities of fucosylated chondroitin sulfates from Holothuria scabra.
29174356	0	57	from	scabra	118:123	arg1	structures					26:35	structures	26:35	structures	26:35	Separation, purification, structures and anticoagulant activities of fucosylated chondroitin sulfates from Holothuria scabra.
29174356	0	57	from	scabra	118:123	arg1	activities					55:64	anticoagulant activities	41:64	anticoagulant activities	41:64	Separation, purification, structures and anticoagulant activities of fucosylated chondroitin sulfates from Holothuria scabra.
29174356	4	58	theme	galactosamine	1242:1254	arg1	groups					1280:1285	the β-d-glucuronic acid, N-acetyl-β-d- galactosamine, α-l-fucose and sulfate groups	1203:1285	the β-d-glucuronic acid, N-acetyl-β-d- galactosamine, α-l-fucose and sulfate groups	1203:1285	The backbone →4)GlcUAβ(1 → 3)GalNAcβ(1→ and sulfated fucose branches were composed of the β-d-glucuronic acid, N-acetyl-β-d- galactosamine, α-l-fucose and sulfate groups by the molar ratio of 1:1.72:2.34:3.29.
29174356	2	59	theme	HsG	317:319	arg1	structure					300:308	the structure	296:308	the structure of the HsG	296:319	We investigated the structure of the HsG by adopting monosaccharide composition analysis, disaccharide composition analysis, IR,1H and13C NMR spectra and methylation analysis.
29174356	3	60	theme	O-3	885:887	arg1	position					889:896	the O-3 position	881:896	the O-3 position of β-d-glucuronic acid	881:919	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	1	61	gly	fucosylated	147:157	arg1	HsG					180:182	HsG	180:182	HsG	180:182	In this study, a new fucosylated chondroitin sulfate (HsG) with an average molecular weight of 69.1 kDa was isolated from sea cucumber Holothuria scabra.
29174356	1	61	gly	fucosylated	147:157	arg1	sulfate					171:177	a new fucosylated chondroitin sulfate	141:177	a new fucosylated chondroitin sulfate (HsG) with an average molecular weight of 69.1 kDa	141:228	In this study, a new fucosylated chondroitin sulfate (HsG) with an average molecular weight of 69.1 kDa was isolated from sea cucumber Holothuria scabra.
29174356	3	62	theme	fucopyranosyl	696:708	arg1	residue					710:716	one fucopyranosyl residue	692:716	one fucopyranosyl residue	692:716	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	3	62	theme	fucopyranosyl	696:708	arg1	wherein					719:725	wherein	719:725	wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone	719:1114	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	1	63	attach	isolated	234:241	arg2	sulfate					171:177	a new fucosylated chondroitin sulfate	141:177	a new fucosylated chondroitin sulfate (HsG) with an average molecular weight of 69.1 kDa	141:228	In this study, a new fucosylated chondroitin sulfate (HsG) with an average molecular weight of 69.1 kDa was isolated from sea cucumber Holothuria scabra.
29174356	1	63	attach	isolated	234:241	arg2	HsG					180:182	HsG	180:182	HsG	180:182	In this study, a new fucosylated chondroitin sulfate (HsG) with an average molecular weight of 69.1 kDa was isolated from sea cucumber Holothuria scabra.
29174356	1	63	attach	isolated	234:241	arg1	cucumber					252:259	sea cucumber	248:259	sea cucumber	248:259	In this study, a new fucosylated chondroitin sulfate (HsG) with an average molecular weight of 69.1 kDa was isolated from sea cucumber Holothuria scabra.
29174356	5	64	theme	anticoagulant	1331:1343	arg1	activities					1345:1354	The anticoagulant activities	1327:1354	The anticoagulant activities of the HsG	1327:1365	The anticoagulant activities of the HsG was evaluated and compared with heparin.
29174356	3	65	theme	desulfated/carboxyl-reduced	492:518	arg1	polysaccharides					520:534	desulfated/carboxyl-reduced polysaccharides	492:534	desulfated/carboxyl-reduced polysaccharides	492:534	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	6	66	theme	activated	1457:1465	arg1	time					1490:1493	the activated partial thromboplastin time	1453:1493	the activated partial thromboplastin time	1453:1493	The result showed that the HsG could prolong the activated partial thromboplastin time.
29174356	0	67	theme	fucosylated	69:79	arg1	sulfates					93:100	fucosylated chondroitin sulfates	69:100	fucosylated chondroitin sulfates	69:100	Separation, purification, structures and anticoagulant activities of fucosylated chondroitin sulfates from Holothuria scabra.
29174356	2	68	theme	IR,1H	405:409	arg1	spectra					422:428	IR,1H and13C NMR spectra	405:428	IR,1H and13C NMR spectra	405:428	We investigated the structure of the HsG by adopting monosaccharide composition analysis, disaccharide composition analysis, IR,1H and13C NMR spectra and methylation analysis.
29174356	3	69	theme	N-acetylgalactosamine	997:1017	arg1	moiety					1019:1024	the N-acetylgalactosamine moiety	993:1024	the N-acetylgalactosamine moiety	993:1024	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	6	70	theme	partial	1467:1473	arg1	time					1490:1493	the activated partial thromboplastin time	1453:1493	the activated partial thromboplastin time	1453:1493	The result showed that the HsG could prolong the activated partial thromboplastin time.
29174356	1	71	theme	69.1 kDa	221:228	arg1	weight					211:216	an average molecular weight	190:216	an average molecular weight of 69.1 kDa	190:228	In this study, a new fucosylated chondroitin sulfate (HsG) with an average molecular weight of 69.1 kDa was isolated from sea cucumber Holothuria scabra.
29174356	4	72	theme	1:1.72:2.34:3.29	1309:1324	arg1	ratio					1300:1304	the molar ratio	1290:1304	the molar ratio of 1:1.72:2.34:3.29	1290:1324	The backbone →4)GlcUAβ(1 → 3)GalNAcβ(1→ and sulfated fucose branches were composed of the β-d-glucuronic acid, N-acetyl-β-d- galactosamine, α-l-fucose and sulfate groups by the molar ratio of 1:1.72:2.34:3.29.
29174356	3	73	dep	residues	949:956	arg1	linked					962:967	linked	962:967	are linked to the O-4 positions of the N-acetylgalactosamine moiety	958:1024	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	1	74	theme	new	143:145	arg1	HsG					180:182	HsG	180:182	HsG	180:182	In this study, a new fucosylated chondroitin sulfate (HsG) with an average molecular weight of 69.1 kDa was isolated from sea cucumber Holothuria scabra.
29174356	1	74	theme	new	143:145	arg1	sulfate					171:177	a new fucosylated chondroitin sulfate	141:177	a new fucosylated chondroitin sulfate (HsG) with an average molecular weight of 69.1 kDa	141:228	In this study, a new fucosylated chondroitin sulfate (HsG) with an average molecular weight of 69.1 kDa was isolated from sea cucumber Holothuria scabra.
29174356	3	75	theme	β-d-glucuronic	901:914	arg1	acid					916:919	β-d-glucuronic acid	901:919	β-d-glucuronic acid	901:919	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
29174356	3	76	dep	residues	754:761	arg1	linked					767:772	linked	767:772	are linked to the O-6 position of the N-acetylgalactosamine moiety	763:828	According to methylation results of desulfated/carboxyl-reduced polysaccharides and the analysis of unsaturated disaccharides generated through the enzymolysis of the defucosed polysaccharides, it is shown that each branch is formed by one fucopyranosyl residue, wherein 55.7% of the fucopyranosyl residues are linked to the O-6 position of the N-acetylgalactosamine moiety, 21.2% of the fucopyranosyl residues are linked to the O-3 position of β-d-glucuronic acid, 13.0% of the fucopyranosyl residues are linked to the O-4 positions of the N-acetylgalactosamine moiety, and 10.1% of the fucopyranosyl residues are not linked to sulfate groups on the backbone.
24663085	5	0	attach	attached	922:929	arg1	position					942:949	the O-6 position	934:949	the O-6 position of the α-D-Glcp residues	934:974	Structural analysis indicated that LP1 consisted of a backbone of → 4)-α-D-Glcp-(1 → 4)-α-D-GALPA-(1 → 4)-α-D-Glcp-(1 → 4)-β-D-Glcp-(1 → units with poly saccharide side chains composed of → 2)-β-D-Fruf-(1 → 2)-L-sorbose-(1 → attached to the O-6 position of the α-D-Glcp residues.
24663085	5	0	attach	attached	922:929	arg2	-L-sorbose-					906:916	-α-D-Glcp-(1 → 4)-α-D-GALPA-(1 → 4)-α-D-Glcp-(1 → 4)-β-D-Glcp-(1 → units with poly saccharide side chains composed of → 2)-β-D-Fruf-(1 → 2)-L-sorbose-	767:916	-α-D-Glcp-(1 → 4)-α-D-GALPA-(1 → 4)-α-D-Glcp-(1 → 4)-β-D-Glcp-(1 → units with poly saccharide side chains composed of → 2)-β-D-Fruf-(1 → 2)-L-sorbose-	767:916	Structural analysis indicated that LP1 consisted of a backbone of → 4)-α-D-Glcp-(1 → 4)-α-D-GALPA-(1 → 4)-α-D-Glcp-(1 → 4)-β-D-Glcp-(1 → units with poly saccharide side chains composed of → 2)-β-D-Fruf-(1 → 2)-L-sorbose-(1 → attached to the O-6 position of the α-D-Glcp residues.
24663085	5	0	attach	attached	922:929	arg2	→					920:920	→	920:920	→	920:920	Structural analysis indicated that LP1 consisted of a backbone of → 4)-α-D-Glcp-(1 → 4)-α-D-GALPA-(1 → 4)-α-D-Glcp-(1 → 4)-β-D-Glcp-(1 → units with poly saccharide side chains composed of → 2)-β-D-Fruf-(1 → 2)-L-sorbose-(1 → attached to the O-6 position of the α-D-Glcp residues.
24663085	5	1	with	units	834:838	arg1	chains					866:871	poly saccharide side chains	845:871	poly saccharide side chains composed of → 2)-β-D-Fruf-(1 → 2	845:904	Structural analysis indicated that LP1 consisted of a backbone of → 4)-α-D-Glcp-(1 → 4)-α-D-GALPA-(1 → 4)-α-D-Glcp-(1 → 4)-β-D-Glcp-(1 → units with poly saccharide side chains composed of → 2)-β-D-Fruf-(1 → 2)-L-sorbose-(1 → attached to the O-6 position of the α-D-Glcp residues.
24663085	6	2	theme	tumor	1084:1088	arg1	cells					1090:1094	SKOV3 and HO8910 tumor cells	1067:1094	cells	1090:1094	In vitro experiments indicated that LP1 had significantly high antitumor activity against SKOV3 and HO8910 tumor cells, with inhibition percentages of 40% and 50%, respectively.
24663085	7	3	theme	B-	1308:1309	arg1	proliferation					1328:1340	B- and T-lymphocyte proliferation	1308:1340	B- and T-lymphocyte proliferation	1308:1340	In addition, LP1 significantly stimulated the production of the cytokine interferon-γ (IFN-γ), increased the activity of murine macrophages and enhanced B- and T-lymphocyte proliferation.
24663085	5	4	dep	-β-D-Fruf-	889:898	arg1	2					887:887	2	887:887	2	887:887	Structural analysis indicated that LP1 consisted of a backbone of → 4)-α-D-Glcp-(1 → 4)-α-D-GALPA-(1 → 4)-α-D-Glcp-(1 → 4)-β-D-Glcp-(1 → units with poly saccharide side chains composed of → 2)-β-D-Fruf-(1 → 2)-L-sorbose-(1 → attached to the O-6 position of the α-D-Glcp residues.
24663085	0	5	from	Structure	0:8	arg1	Dimocarpus					95:104	Dimocarpus	95:104	Dimocarpus	95:104	Structure and antitumor and immunomodulatory activities of a water-soluble polysaccharide from Dimocarpus longan pulp.
24663085	6	6	theme	HO8910	1077:1082	arg1	cells					1090:1094	SKOV3 and HO8910 tumor cells	1067:1094	cells	1090:1094	In vitro experiments indicated that LP1 had significantly high antitumor activity against SKOV3 and HO8910 tumor cells, with inhibition percentages of 40% and 50%, respectively.
24663085	1	7	dep	polysaccharide	139:152	arg1	polysaccharide					162:175	longan polysaccharide 1	155:177	longan polysaccharide 1 (LP1)	155:183	A new water-soluble polysaccharide (longan polysaccharide 1 (LP1)) was extracted and successfully purified from Dimocarpus longan pulp via diethylaminoethyl (DEAE)-cellulose anion-exchange and Sephacryl S-300 HR gel chromatography.
24663085	1	7	dep	polysaccharide	139:152	arg1	LP1					180:182	LP1	180:182	LP1	180:182	A new water-soluble polysaccharide (longan polysaccharide 1 (LP1)) was extracted and successfully purified from Dimocarpus longan pulp via diethylaminoethyl (DEAE)-cellulose anion-exchange and Sephacryl S-300 HR gel chromatography.
24663085	8	8	theme	natural	1422:1428	arg1	agent					1440:1444	a natural antitumor agent	1420:1444	a natural antitumor agent with immunomodulatory activity	1420:1475	The results of this study demonstrate that LP1 has potential applications as a natural antitumor agent with immunomodulatory activity.
24663085	8	8	theme	natural	1422:1428	arg1	applications					1404:1415	potential applications	1394:1415	potential applications	1394:1415	The results of this study demonstrate that LP1 has potential applications as a natural antitumor agent with immunomodulatory activity.
24663085	8	9	theme	potential	1394:1402	arg1	agent					1440:1444	a natural antitumor agent	1420:1444	a natural antitumor agent with immunomodulatory activity	1420:1475	The results of this study demonstrate that LP1 has potential applications as a natural antitumor agent with immunomodulatory activity.
24663085	8	9	theme	potential	1394:1402	arg1	applications					1404:1415	potential applications	1394:1415	potential applications	1394:1415	The results of this study demonstrate that LP1 has potential applications as a natural antitumor agent with immunomodulatory activity.
24663085	7	10	theme	cytokine	1219:1226	arg1	IFN-γ					1242:1246	IFN-γ	1242:1246	IFN-γ	1242:1246	In addition, LP1 significantly stimulated the production of the cytokine interferon-γ (IFN-γ), increased the activity of murine macrophages and enhanced B- and T-lymphocyte proliferation.
24663085	7	10	theme	cytokine	1219:1226	arg1	interferon-γ					1228:1239	the cytokine interferon-γ	1215:1239	the cytokine interferon-γ (IFN-γ)	1215:1247	In addition, LP1 significantly stimulated the production of the cytokine interferon-γ (IFN-γ), increased the activity of murine macrophages and enhanced B- and T-lymphocyte proliferation.
24663085	6	11	theme	%	1138:1138	arg1	percentages					1113:1123	inhibition percentages	1102:1123	inhibition percentages of 40% and 50%, respectively	1102:1152	In vitro experiments indicated that LP1 had significantly high antitumor activity against SKOV3 and HO8910 tumor cells, with inhibition percentages of 40% and 50%, respectively.
24663085	2	12	theme	chemical	355:362	arg1	structure					364:372	The chemical structure	351:372	The chemical structure	351:372	The chemical structure was determined using Infrared (IR), gas chromatography (GC) and nuclear magnetic resonance (NMR) analysis.
24663085	5	13	dep	consisted	736:744	arg1	attached					922:929	attached	922:929	attached to the O-6 position of the α-D-Glcp residues	922:974	Structural analysis indicated that LP1 consisted of a backbone of → 4)-α-D-Glcp-(1 → 4)-α-D-GALPA-(1 → 4)-α-D-Glcp-(1 → 4)-β-D-Glcp-(1 → units with poly saccharide side chains composed of → 2)-β-D-Fruf-(1 → 2)-L-sorbose-(1 → attached to the O-6 position of the α-D-Glcp residues.
24663085	3	14	theme	molecular	512:520	arg1	weight					522:527	the molecular weight	508:527	the molecular weight of the sample	508:541	The results indicated that the molecular weight of the sample was 1.1 × 10(5) Da.
24663085	3	14	theme	molecular	512:520	arg1	Da					559:560	1.1 × 10(5) Da	547:560	1.1 × 10(5) Da	547:560	The results indicated that the molecular weight of the sample was 1.1 × 10(5) Da.
24663085	0	15	from	activities	45:54	arg1	Dimocarpus					95:104	Dimocarpus	95:104	Dimocarpus	95:104	Structure and antitumor and immunomodulatory activities of a water-soluble polysaccharide from Dimocarpus longan pulp.
24663085	8	16	contain	has	1390:1392	arg1	LP1					1386:1388	LP1	1386:1388	LP1	1386:1388	The results of this study demonstrate that LP1 has potential applications as a natural antitumor agent with immunomodulatory activity.
24663085	8	16	contain	has	1390:1392	arg2	agent					1440:1444	a natural antitumor agent	1420:1444	a natural antitumor agent with immunomodulatory activity	1420:1475	The results of this study demonstrate that LP1 has potential applications as a natural antitumor agent with immunomodulatory activity.
24663085	8	16	contain	has	1390:1392	arg2	applications					1404:1415	potential applications	1394:1415	potential applications	1394:1415	The results of this study demonstrate that LP1 has potential applications as a natural antitumor agent with immunomodulatory activity.
24663085	5	17	theme	→	832:832	arg1	units					834:838	1 → units	830:838	1 → units with poly saccharide side chains composed of → 2)-β-D-Fruf-(1 → 2	830:904	Structural analysis indicated that LP1 consisted of a backbone of → 4)-α-D-Glcp-(1 → 4)-α-D-GALPA-(1 → 4)-α-D-Glcp-(1 → 4)-β-D-Glcp-(1 → units with poly saccharide side chains composed of → 2)-β-D-Fruf-(1 → 2)-L-sorbose-(1 → attached to the O-6 position of the α-D-Glcp residues.
24663085	6	18	contain	had	1017:1019	arg2	activity					1050:1057	significantly high antitumor activity	1021:1057	significantly high antitumor activity against SKOV3 and HO8910 tumor cells	1021:1094	In vitro experiments indicated that LP1 had significantly high antitumor activity against SKOV3 and HO8910 tumor cells, with inhibition percentages of 40% and 50%, respectively.
24663085	6	18	contain	had	1017:1019	arg1	LP1					1013:1015	LP1	1013:1015	LP1	1013:1015	In vitro experiments indicated that LP1 had significantly high antitumor activity against SKOV3 and HO8910 tumor cells, with inhibition percentages of 40% and 50%, respectively.
24663085	2	19	theme	nuclear	438:444	arg1	resonance					455:463	nuclear magnetic resonance	438:463	nuclear magnetic resonance (NMR) analysis	438:478	The chemical structure was determined using Infrared (IR), gas chromatography (GC) and nuclear magnetic resonance (NMR) analysis.
24663085	2	19	theme	nuclear	438:444	arg1	NMR					466:468	NMR	466:468	NMR	466:468	The chemical structure was determined using Infrared (IR), gas chromatography (GC) and nuclear magnetic resonance (NMR) analysis.
24663085	7	20	theme	macrophages	1283:1293	arg1	activity					1264:1271	the activity	1260:1271	the activity of murine macrophages	1260:1293	In addition, LP1 significantly stimulated the production of the cytokine interferon-γ (IFN-γ), increased the activity of murine macrophages and enhanced B- and T-lymphocyte proliferation.
24663085	5	21	theme	α-D-Glcp	958:965	arg1	residues					967:974	the α-D-Glcp residues	954:974	the α-D-Glcp residues	954:974	Structural analysis indicated that LP1 consisted of a backbone of → 4)-α-D-Glcp-(1 → 4)-α-D-GALPA-(1 → 4)-α-D-Glcp-(1 → 4)-β-D-Glcp-(1 → units with poly saccharide side chains composed of → 2)-β-D-Fruf-(1 → 2)-L-sorbose-(1 → attached to the O-6 position of the α-D-Glcp residues.
24663085	6	22	theme	antitumor	1040:1048	arg1	activity					1050:1057	significantly high antitumor activity	1021:1057	significantly high antitumor activity against SKOV3 and HO8910 tumor cells	1021:1094	In vitro experiments indicated that LP1 had significantly high antitumor activity against SKOV3 and HO8910 tumor cells, with inhibition percentages of 40% and 50%, respectively.
24663085	3	23	theme	×	551:551	arg1	weight					522:527	the molecular weight	508:527	the molecular weight of the sample	508:541	The results indicated that the molecular weight of the sample was 1.1 × 10(5) Da.
24663085	3	23	theme	×	551:551	arg1	Da					559:560	1.1 × 10(5) Da	547:560	1.1 × 10(5) Da	547:560	The results indicated that the molecular weight of the sample was 1.1 × 10(5) Da.
24663085	7	24	theme	T-lymphocyte	1315:1326	arg1	proliferation					1328:1340	B- and T-lymphocyte proliferation	1308:1340	B- and T-lymphocyte proliferation	1308:1340	In addition, LP1 significantly stimulated the production of the cytokine interferon-γ (IFN-γ), increased the activity of murine macrophages and enhanced B- and T-lymphocyte proliferation.
24663085	4	25	theme	molar	661:665	arg1	ratio					667:671	a molar ratio	659:671	a molar ratio of 5.39:1.04:0.74:0.21	659:694	Monosaccharide composition analysis revealed that LP1 was composed of Glc, GalA, Ara and Gal in a molar ratio of 5.39:1.04:0.74:0.21.
24663085	3	26	theme	sample	536:541	arg1	weight					522:527	the molecular weight	508:527	the molecular weight of the sample	508:541	The results indicated that the molecular weight of the sample was 1.1 × 10(5) Da.
24663085	3	26	theme	sample	536:541	arg1	Da					559:560	1.1 × 10(5) Da	547:560	1.1 × 10(5) Da	547:560	The results indicated that the molecular weight of the sample was 1.1 × 10(5) Da.
24663085	6	27	theme	high	1035:1038	arg1	activity					1050:1057	significantly high antitumor activity	1021:1057	significantly high antitumor activity against SKOV3 and HO8910 tumor cells	1021:1094	In vitro experiments indicated that LP1 had significantly high antitumor activity against SKOV3 and HO8910 tumor cells, with inhibition percentages of 40% and 50%, respectively.
24663085	5	28	theme	-β-D-Glcp-	819:828	arg1	-L-sorbose-					906:916	-α-D-Glcp-(1 → 4)-α-D-GALPA-(1 → 4)-α-D-Glcp-(1 → 4)-β-D-Glcp-(1 → units with poly saccharide side chains composed of → 2)-β-D-Fruf-(1 → 2)-L-sorbose-	767:916	-α-D-Glcp-(1 → 4)-α-D-GALPA-(1 → 4)-α-D-Glcp-(1 → 4)-β-D-Glcp-(1 → units with poly saccharide side chains composed of → 2)-β-D-Fruf-(1 → 2)-L-sorbose-	767:916	Structural analysis indicated that LP1 consisted of a backbone of → 4)-α-D-Glcp-(1 → 4)-α-D-GALPA-(1 → 4)-α-D-Glcp-(1 → 4)-β-D-Glcp-(1 → units with poly saccharide side chains composed of → 2)-β-D-Fruf-(1 → 2)-L-sorbose-(1 → attached to the O-6 position of the α-D-Glcp residues.
24663085	0	29	theme	antitumor	14:22	arg1	activities					45:54	antitumor and immunomodulatory activities	14:54	activities	45:54	Structure and antitumor and immunomodulatory activities of a water-soluble polysaccharide from Dimocarpus longan pulp.
24663085	6	30	theme	In	977:978	arg1	experiments					986:996	In vitro experiments	977:996	In vitro experiments	977:996	In vitro experiments indicated that LP1 had significantly high antitumor activity against SKOV3 and HO8910 tumor cells, with inhibition percentages of 40% and 50%, respectively.
24663085	6	31	theme	%	1130:1130	arg1	percentages					1113:1123	inhibition percentages	1102:1123	inhibition percentages of 40% and 50%, respectively	1102:1152	In vitro experiments indicated that LP1 had significantly high antitumor activity against SKOV3 and HO8910 tumor cells, with inhibition percentages of 40% and 50%, respectively.
24663085	0	32	from	Dimocarpus	95:104	arg1	activities					45:54	antitumor and immunomodulatory activities	14:54	activities	45:54	Structure and antitumor and immunomodulatory activities of a water-soluble polysaccharide from Dimocarpus longan pulp.
24663085	0	32	from	Dimocarpus	95:104	arg1	Structure					0:8	Structure	0:8	Structure	0:8	Structure and antitumor and immunomodulatory activities of a water-soluble polysaccharide from Dimocarpus longan pulp.
24663085	0	32	from	Dimocarpus	95:104	arg1	polysaccharide					75:88	a water-soluble polysaccharide	59:88	a water-soluble polysaccharide from Dimocarpus	59:104	Structure and antitumor and immunomodulatory activities of a water-soluble polysaccharide from Dimocarpus longan pulp.
24663085	5	33	dep	attached	922:929	arg1	4					765:765	4	765:765	4	765:765	Structural analysis indicated that LP1 consisted of a backbone of → 4)-α-D-Glcp-(1 → 4)-α-D-GALPA-(1 → 4)-α-D-Glcp-(1 → 4)-β-D-Glcp-(1 → units with poly saccharide side chains composed of → 2)-β-D-Fruf-(1 → 2)-L-sorbose-(1 → attached to the O-6 position of the α-D-Glcp residues.
24663085	5	33	dep	attached	922:929	arg1	1					918:918	1	918:918	1	918:918	Structural analysis indicated that LP1 consisted of a backbone of → 4)-α-D-Glcp-(1 → 4)-α-D-GALPA-(1 → 4)-α-D-Glcp-(1 → 4)-β-D-Glcp-(1 → units with poly saccharide side chains composed of → 2)-β-D-Fruf-(1 → 2)-L-sorbose-(1 → attached to the O-6 position of the α-D-Glcp residues.
24663085	8	34	with	agent	1440:1444	arg1	activity					1468:1475	immunomodulatory activity	1451:1475	immunomodulatory activity	1451:1475	The results of this study demonstrate that LP1 has potential applications as a natural antitumor agent with immunomodulatory activity.
24663085	6	35	dep	In	977:978	arg1	vitro					980:984	vitro	980:984	vitro	980:984	In vitro experiments indicated that LP1 had significantly high antitumor activity against SKOV3 and HO8910 tumor cells, with inhibition percentages of 40% and 50%, respectively.
24663085	5	36	theme	side	861:864	arg1	chains					866:871	poly saccharide side chains	845:871	poly saccharide side chains composed of → 2)-β-D-Fruf-(1 → 2	845:904	Structural analysis indicated that LP1 consisted of a backbone of → 4)-α-D-Glcp-(1 → 4)-α-D-GALPA-(1 → 4)-α-D-Glcp-(1 → 4)-β-D-Glcp-(1 → units with poly saccharide side chains composed of → 2)-β-D-Fruf-(1 → 2)-L-sorbose-(1 → attached to the O-6 position of the α-D-Glcp residues.
24663085	2	37	theme	gas	410:412	arg1	GC					430:431	GC	430:431	GC	430:431	The chemical structure was determined using Infrared (IR), gas chromatography (GC) and nuclear magnetic resonance (NMR) analysis.
24663085	2	37	theme	gas	410:412	arg1	chromatography					414:427	gas chromatography	410:427	gas chromatography (GC)	410:432	The chemical structure was determined using Infrared (IR), gas chromatography (GC) and nuclear magnetic resonance (NMR) analysis.
24663085	0	38	theme	immunomodulatory	28:43	arg1	activities					45:54	antitumor and immunomodulatory activities	14:54	activities	45:54	Structure and antitumor and immunomodulatory activities of a water-soluble polysaccharide from Dimocarpus longan pulp.
24663085	1	39	theme	Sephacryl	312:320	arg1	chromatography					335:348	diethylaminoethyl (DEAE)-cellulose anion-exchange and Sephacryl S-300 HR gel chromatography	258:348	chromatography	335:348	A new water-soluble polysaccharide (longan polysaccharide 1 (LP1)) was extracted and successfully purified from Dimocarpus longan pulp via diethylaminoethyl (DEAE)-cellulose anion-exchange and Sephacryl S-300 HR gel chromatography.
24663085	4	40	theme	composition	578:588	arg1	analysis					590:597	Monosaccharide composition analysis	563:597	Monosaccharide composition analysis	563:597	Monosaccharide composition analysis revealed that LP1 was composed of Glc, GalA, Ara and Gal in a molar ratio of 5.39:1.04:0.74:0.21.
24663085	8	41	theme	study	1363:1367	arg1	results					1347:1353	The results	1343:1353	The results of this study	1343:1367	The results of this study demonstrate that LP1 has potential applications as a natural antitumor agent with immunomodulatory activity.
24663085	2	42	theme	resonance	455:463	arg1	analysis					471:478	nuclear magnetic resonance (NMR) analysis	438:478	nuclear magnetic resonance (NMR) analysis	438:478	The chemical structure was determined using Infrared (IR), gas chromatography (GC) and nuclear magnetic resonance (NMR) analysis.
24663085	1	43	theme	S-300	322:326	arg1	chromatography					335:348	diethylaminoethyl (DEAE)-cellulose anion-exchange and Sephacryl S-300 HR gel chromatography	258:348	chromatography	335:348	A new water-soluble polysaccharide (longan polysaccharide 1 (LP1)) was extracted and successfully purified from Dimocarpus longan pulp via diethylaminoethyl (DEAE)-cellulose anion-exchange and Sephacryl S-300 HR gel chromatography.
24663085	5	44	theme	residues	967:974	arg1	position					942:949	the O-6 position	934:949	the O-6 position of the α-D-Glcp residues	934:974	Structural analysis indicated that LP1 consisted of a backbone of → 4)-α-D-Glcp-(1 → 4)-α-D-GALPA-(1 → 4)-α-D-Glcp-(1 → 4)-β-D-Glcp-(1 → units with poly saccharide side chains composed of → 2)-β-D-Fruf-(1 → 2)-L-sorbose-(1 → attached to the O-6 position of the α-D-Glcp residues.
24663085	7	45	theme	interferon-γ	1228:1239	arg1	production					1201:1210	the production	1197:1210	the production of the cytokine interferon-γ (IFN-γ)	1197:1247	In addition, LP1 significantly stimulated the production of the cytokine interferon-γ (IFN-γ), increased the activity of murine macrophages and enhanced B- and T-lymphocyte proliferation.
24663085	1	46	theme	new	121:123	arg1	polysaccharide					139:152	A new water-soluble polysaccharide	119:152	A new water-soluble polysaccharide (longan polysaccharide 1 (LP1))	119:184	A new water-soluble polysaccharide (longan polysaccharide 1 (LP1)) was extracted and successfully purified from Dimocarpus longan pulp via diethylaminoethyl (DEAE)-cellulose anion-exchange and Sephacryl S-300 HR gel chromatography.
24663085	2	47	theme	magnetic	446:453	arg1	resonance					455:463	nuclear magnetic resonance	438:463	nuclear magnetic resonance (NMR) analysis	438:478	The chemical structure was determined using Infrared (IR), gas chromatography (GC) and nuclear magnetic resonance (NMR) analysis.
24663085	2	47	theme	magnetic	446:453	arg1	NMR					466:468	NMR	466:468	NMR	466:468	The chemical structure was determined using Infrared (IR), gas chromatography (GC) and nuclear magnetic resonance (NMR) analysis.
24663085	1	48	theme	HR	328:329	arg1	chromatography					335:348	diethylaminoethyl (DEAE)-cellulose anion-exchange and Sephacryl S-300 HR gel chromatography	258:348	chromatography	335:348	A new water-soluble polysaccharide (longan polysaccharide 1 (LP1)) was extracted and successfully purified from Dimocarpus longan pulp via diethylaminoethyl (DEAE)-cellulose anion-exchange and Sephacryl S-300 HR gel chromatography.
24663085	6	49	theme	inhibition	1102:1111	arg1	percentages					1113:1123	inhibition percentages	1102:1123	inhibition percentages of 40% and 50%, respectively	1102:1152	In vitro experiments indicated that LP1 had significantly high antitumor activity against SKOV3 and HO8910 tumor cells, with inhibition percentages of 40% and 50%, respectively.
24663085	5	50	theme	→	885:885	arg1	-β-D-Fruf-					889:898	→ 2)-β-D-Fruf-(1 → 2	885:904	→ 2)-β-D-Fruf-(1 → 2	885:904	Structural analysis indicated that LP1 consisted of a backbone of → 4)-α-D-Glcp-(1 → 4)-α-D-GALPA-(1 → 4)-α-D-Glcp-(1 → 4)-β-D-Glcp-(1 → units with poly saccharide side chains composed of → 2)-β-D-Fruf-(1 → 2)-L-sorbose-(1 → attached to the O-6 position of the α-D-Glcp residues.
24663085	5	51	dep	→	780:780	arg1	-α-D-Glcp-					802:811	-α-D-Glcp-(1 → 4	802:817	1 → 4)-α-D-GALPA-(1 → 4)-α-D-Glcp-(1 → 4	778:817	Structural analysis indicated that LP1 consisted of a backbone of → 4)-α-D-Glcp-(1 → 4)-α-D-GALPA-(1 → 4)-α-D-Glcp-(1 → 4)-β-D-Glcp-(1 → units with poly saccharide side chains composed of → 2)-β-D-Fruf-(1 → 2)-L-sorbose-(1 → attached to the O-6 position of the α-D-Glcp residues.
24663085	5	51	dep	→	780:780	arg1	-α-D-GALPA-					784:794	-α-D-GALPA-(1 → 4)	784:801	1 → 4)-α-D-GALPA-(1 → 4)-α-D-Glcp-(1 → 4	778:817	Structural analysis indicated that LP1 consisted of a backbone of → 4)-α-D-Glcp-(1 → 4)-α-D-GALPA-(1 → 4)-α-D-Glcp-(1 → 4)-β-D-Glcp-(1 → units with poly saccharide side chains composed of → 2)-β-D-Fruf-(1 → 2)-L-sorbose-(1 → attached to the O-6 position of the α-D-Glcp residues.
24663085	5	51	dep	→	780:780	arg1	4					782:782	4	782:782	4	782:782	Structural analysis indicated that LP1 consisted of a backbone of → 4)-α-D-Glcp-(1 → 4)-α-D-GALPA-(1 → 4)-α-D-Glcp-(1 → 4)-β-D-Glcp-(1 → units with poly saccharide side chains composed of → 2)-β-D-Fruf-(1 → 2)-L-sorbose-(1 → attached to the O-6 position of the α-D-Glcp residues.
24663085	5	52	theme	O-6	938:940	arg1	position					942:949	the O-6 position	934:949	the O-6 position of the α-D-Glcp residues	934:974	Structural analysis indicated that LP1 consisted of a backbone of → 4)-α-D-Glcp-(1 → 4)-α-D-GALPA-(1 → 4)-α-D-Glcp-(1 → 4)-β-D-Glcp-(1 → units with poly saccharide side chains composed of → 2)-β-D-Fruf-(1 → 2)-L-sorbose-(1 → attached to the O-6 position of the α-D-Glcp residues.
24663085	1	53	theme	water-soluble	125:137	arg1	polysaccharide					139:152	A new water-soluble polysaccharide	119:152	A new water-soluble polysaccharide (longan polysaccharide 1 (LP1))	119:184	A new water-soluble polysaccharide (longan polysaccharide 1 (LP1)) was extracted and successfully purified from Dimocarpus longan pulp via diethylaminoethyl (DEAE)-cellulose anion-exchange and Sephacryl S-300 HR gel chromatography.
24663085	1	54	theme	gel	331:333	arg1	chromatography					335:348	diethylaminoethyl (DEAE)-cellulose anion-exchange and Sephacryl S-300 HR gel chromatography	258:348	chromatography	335:348	A new water-soluble polysaccharide (longan polysaccharide 1 (LP1)) was extracted and successfully purified from Dimocarpus longan pulp via diethylaminoethyl (DEAE)-cellulose anion-exchange and Sephacryl S-300 HR gel chromatography.
24663085	5	55	theme	Structural	697:706	arg1	analysis					708:715	Structural analysis	697:715	Structural analysis	697:715	Structural analysis indicated that LP1 consisted of a backbone of → 4)-α-D-Glcp-(1 → 4)-α-D-GALPA-(1 → 4)-α-D-Glcp-(1 → 4)-β-D-Glcp-(1 → units with poly saccharide side chains composed of → 2)-β-D-Fruf-(1 → 2)-L-sorbose-(1 → attached to the O-6 position of the α-D-Glcp residues.
24663085	5	56	dep	-β-D-Glcp-	819:828	arg1	→					780:780	1 → 4)-α-D-GALPA-(1 → 4)-α-D-Glcp-(1 → 4	778:817	1 → 4)-α-D-GALPA-(1 → 4)-α-D-Glcp-(1 → 4	778:817	Structural analysis indicated that LP1 consisted of a backbone of → 4)-α-D-Glcp-(1 → 4)-α-D-GALPA-(1 → 4)-α-D-Glcp-(1 → 4)-β-D-Glcp-(1 → units with poly saccharide side chains composed of → 2)-β-D-Fruf-(1 → 2)-L-sorbose-(1 → attached to the O-6 position of the α-D-Glcp residues.
24663085	5	56	dep	-β-D-Glcp-	819:828	arg1	units					834:838	1 → units	830:838	1 → units with poly saccharide side chains composed of → 2)-β-D-Fruf-(1 → 2	830:904	Structural analysis indicated that LP1 consisted of a backbone of → 4)-α-D-Glcp-(1 → 4)-α-D-GALPA-(1 → 4)-α-D-Glcp-(1 → 4)-β-D-Glcp-(1 → units with poly saccharide side chains composed of → 2)-β-D-Fruf-(1 → 2)-L-sorbose-(1 → attached to the O-6 position of the α-D-Glcp residues.
24663085	4	57	theme	5.39:1.04:0.74:0.21	676:694	arg1	ratio					667:671	a molar ratio	659:671	a molar ratio of 5.39:1.04:0.74:0.21	659:694	Monosaccharide composition analysis revealed that LP1 was composed of Glc, GalA, Ara and Gal in a molar ratio of 5.39:1.04:0.74:0.21.
24663085	6	58	theme	SKOV3	1067:1071	arg1	cells					1090:1094	SKOV3 and HO8910 tumor cells	1067:1094	cells	1090:1094	In vitro experiments indicated that LP1 had significantly high antitumor activity against SKOV3 and HO8910 tumor cells, with inhibition percentages of 40% and 50%, respectively.
24663085	5	59	theme	→	763:763	arg1	backbone					751:758	a backbone	749:758	a backbone of →	749:763	Structural analysis indicated that LP1 consisted of a backbone of → 4)-α-D-Glcp-(1 → 4)-α-D-GALPA-(1 → 4)-α-D-Glcp-(1 → 4)-β-D-Glcp-(1 → units with poly saccharide side chains composed of → 2)-β-D-Fruf-(1 → 2)-L-sorbose-(1 → attached to the O-6 position of the α-D-Glcp residues.
24663085	8	60	theme	immunomodulatory	1451:1466	arg1	activity					1468:1475	immunomodulatory activity	1451:1475	immunomodulatory activity	1451:1475	The results of this study demonstrate that LP1 has potential applications as a natural antitumor agent with immunomodulatory activity.
24663085	1	61	theme	Dimocarpus	231:240	arg1	pulp					249:252	Dimocarpus longan pulp	231:252	Dimocarpus longan pulp via diethylaminoethyl (DEAE)-cellulose anion-exchange and Sephacryl S-300 HR gel chromatography	231:348	A new water-soluble polysaccharide (longan polysaccharide 1 (LP1)) was extracted and successfully purified from Dimocarpus longan pulp via diethylaminoethyl (DEAE)-cellulose anion-exchange and Sephacryl S-300 HR gel chromatography.
24663085	0	62	theme	polysaccharide	75:88	arg1	activities					45:54	antitumor and immunomodulatory activities	14:54	activities	45:54	Structure and antitumor and immunomodulatory activities of a water-soluble polysaccharide from Dimocarpus longan pulp.
24663085	0	62	theme	polysaccharide	75:88	arg1	Structure					0:8	Structure	0:8	Structure	0:8	Structure and antitumor and immunomodulatory activities of a water-soluble polysaccharide from Dimocarpus longan pulp.
24663085	7	63	theme	murine	1276:1281	arg1	macrophages					1283:1293	murine macrophages	1276:1293	murine macrophages	1276:1293	In addition, LP1 significantly stimulated the production of the cytokine interferon-γ (IFN-γ), increased the activity of murine macrophages and enhanced B- and T-lymphocyte proliferation.
24663085	5	64	theme	poly	845:848	arg1	chains					866:871	poly saccharide side chains	845:871	poly saccharide side chains composed of → 2)-β-D-Fruf-(1 → 2	845:904	Structural analysis indicated that LP1 consisted of a backbone of → 4)-α-D-Glcp-(1 → 4)-α-D-GALPA-(1 → 4)-α-D-Glcp-(1 → 4)-β-D-Glcp-(1 → units with poly saccharide side chains composed of → 2)-β-D-Fruf-(1 → 2)-L-sorbose-(1 → attached to the O-6 position of the α-D-Glcp residues.
24663085	1	65	theme	longan	155:160	arg1	polysaccharide					162:175	longan polysaccharide 1	155:177	longan polysaccharide 1 (LP1)	155:183	A new water-soluble polysaccharide (longan polysaccharide 1 (LP1)) was extracted and successfully purified from Dimocarpus longan pulp via diethylaminoethyl (DEAE)-cellulose anion-exchange and Sephacryl S-300 HR gel chromatography.
24663085	1	65	theme	longan	155:160	arg1	LP1					180:182	LP1	180:182	LP1	180:182	A new water-soluble polysaccharide (longan polysaccharide 1 (LP1)) was extracted and successfully purified from Dimocarpus longan pulp via diethylaminoethyl (DEAE)-cellulose anion-exchange and Sephacryl S-300 HR gel chromatography.
24663085	8	66	theme	antitumor	1430:1438	arg1	agent					1440:1444	a natural antitumor agent	1420:1444	a natural antitumor agent with immunomodulatory activity	1420:1475	The results of this study demonstrate that LP1 has potential applications as a natural antitumor agent with immunomodulatory activity.
24663085	8	66	theme	antitumor	1430:1438	arg1	applications					1404:1415	potential applications	1394:1415	potential applications	1394:1415	The results of this study demonstrate that LP1 has potential applications as a natural antitumor agent with immunomodulatory activity.
24663085	1	67	theme	longan	242:247	arg1	pulp					249:252	Dimocarpus longan pulp	231:252	Dimocarpus longan pulp via diethylaminoethyl (DEAE)-cellulose anion-exchange and Sephacryl S-300 HR gel chromatography	231:348	A new water-soluble polysaccharide (longan polysaccharide 1 (LP1)) was extracted and successfully purified from Dimocarpus longan pulp via diethylaminoethyl (DEAE)-cellulose anion-exchange and Sephacryl S-300 HR gel chromatography.
24663085	0	68	theme	water-soluble	61:73	arg1	polysaccharide					75:88	a water-soluble polysaccharide	59:88	a water-soluble polysaccharide from Dimocarpus	59:104	Structure and antitumor and immunomodulatory activities of a water-soluble polysaccharide from Dimocarpus longan pulp.
24663085	5	69	theme	saccharide	850:859	arg1	chains					866:871	poly saccharide side chains	845:871	poly saccharide side chains composed of → 2)-β-D-Fruf-(1 → 2	845:904	Structural analysis indicated that LP1 consisted of a backbone of → 4)-α-D-Glcp-(1 → 4)-α-D-GALPA-(1 → 4)-α-D-Glcp-(1 → 4)-β-D-Glcp-(1 → units with poly saccharide side chains composed of → 2)-β-D-Fruf-(1 → 2)-L-sorbose-(1 → attached to the O-6 position of the α-D-Glcp residues.
24663085	4	70	theme	Monosaccharide	563:576	arg1	analysis					590:597	Monosaccharide composition analysis	563:597	Monosaccharide composition analysis	563:597	Monosaccharide composition analysis revealed that LP1 was composed of Glc, GalA, Ara and Gal in a molar ratio of 5.39:1.04:0.74:0.21.
29286349	0	0	theme	Sargassum	104:112	arg1	hemiphyllum					114:124	the Brown Seaweed Sargassum hemiphyllum	86:124	the Brown Seaweed Sargassum hemiphyllum	86:124	Compressional-Puffing Pretreatment Enhances Neuroprotective Effects of Fucoidans from the Brown Seaweed Sargassum hemiphyllum on 6-Hydroxydopamine-Induced Apoptosis in SH-SY5Y Cells.
29286349	7	1	theme	SH1-SH3	1274:1280	arg1	effects					1263:1269	the opposing effects	1250:1269	the opposing effects of SH1-SH3 on 6-OHDA-induced neurotoxicity	1250:1312	Additional experiments revealed that phosphorylation of Akt is involved in the opposing effects of SH1-SH3 on 6-OHDA-induced neurotoxicity.
29286349	3	2	theme	sulfate/fucose	642:655	arg1	yield					594:598	the extraction yield	579:598	the extraction yield	579:598	The results indicate that CPP increased the extraction yield, total sugar content, and molar ratios of sulfate/fucose of fucoidan and decreased molecular weight and impurities of fucoidan.
29286349	3	2	theme	sulfate/fucose	642:655	arg1	ratios					632:637	molar ratios	626:637	molar ratios	626:637	The results indicate that CPP increased the extraction yield, total sugar content, and molar ratios of sulfate/fucose of fucoidan and decreased molecular weight and impurities of fucoidan.
29286349	3	2	theme	sulfate/fucose	642:655	arg1	content					613:619	total sugar content	601:619	total sugar content	601:619	The results indicate that CPP increased the extraction yield, total sugar content, and molar ratios of sulfate/fucose of fucoidan and decreased molecular weight and impurities of fucoidan.
29286349	6	3	theme	cell	1059:1062	arg1	distribution					1070:1081	cell cycle distribution	1059:1081	cell cycle distribution	1059:1081	The SH1-SH3 extracts protected SH-SY5Y cells from 6-hydroxydopamine (6-OHDA)-induced apoptosis as illustrated by cell cycle distribution, cytochrome c release, activation of caspase-8, -9, and -3, and DNA fragmentation analyses.
29286349	8	4	theme	diseases	1641:1648	arg1	therapy					1612:1618	the preventive therapy	1597:1618	the preventive therapy of neurodegenerative diseases	1597:1648	SH3 exhibited a relatively high extraction yield, the lowest levels of impurities, and was the most effective at reversing the 6-OHDA-induced neurotoxicity of SH-SY5Y cells among SH1-SH3, which taken together indicate that it may have potential as a candidate therapeutic agent for the preventive therapy of neurodegenerative diseases.
29286349	0	5	theme	Seaweed	96:102	arg1	hemiphyllum					114:124	the Brown Seaweed Sargassum hemiphyllum	86:124	the Brown Seaweed Sargassum hemiphyllum	86:124	Compressional-Puffing Pretreatment Enhances Neuroprotective Effects of Fucoidans from the Brown Seaweed Sargassum hemiphyllum on 6-Hydroxydopamine-Induced Apoptosis in SH-SY5Y Cells.
29286349	8	6	theme	SH-SY5Y	1474:1480	arg1	cells					1482:1486	SH-SY5Y cells	1474:1486	SH-SY5Y cells	1474:1486	SH3 exhibited a relatively high extraction yield, the lowest levels of impurities, and was the most effective at reversing the 6-OHDA-induced neurotoxicity of SH-SY5Y cells among SH1-SH3, which taken together indicate that it may have potential as a candidate therapeutic agent for the preventive therapy of neurodegenerative diseases.
29286349	6	7	theme	SH1-SH3	950:956	arg1	extracts					958:965	The SH1-SH3 extracts	946:965	The SH1-SH3 extracts	946:965	The SH1-SH3 extracts protected SH-SY5Y cells from 6-hydroxydopamine (6-OHDA)-induced apoptosis as illustrated by cell cycle distribution, cytochrome c release, activation of caspase-8, -9, and -3, and DNA fragmentation analyses.
29286349	8	8	theme	high	1342:1345	arg1	yield					1358:1362	a relatively high extraction yield	1329:1362	a relatively high extraction yield	1329:1362	SH3 exhibited a relatively high extraction yield, the lowest levels of impurities, and was the most effective at reversing the 6-OHDA-induced neurotoxicity of SH-SY5Y cells among SH1-SH3, which taken together indicate that it may have potential as a candidate therapeutic agent for the preventive therapy of neurodegenerative diseases.
29286349	8	8	theme	high	1342:1345	arg1	levels					1376:1381	the lowest levels	1365:1381	the lowest levels of impurities	1365:1395	SH3 exhibited a relatively high extraction yield, the lowest levels of impurities, and was the most effective at reversing the 6-OHDA-induced neurotoxicity of SH-SY5Y cells among SH1-SH3, which taken together indicate that it may have potential as a candidate therapeutic agent for the preventive therapy of neurodegenerative diseases.
29286349	8	9	theme	candidate	1565:1573	arg1	agent					1587:1591	a candidate therapeutic agent	1563:1591	a candidate therapeutic agent for the preventive therapy of neurodegenerative diseases	1563:1648	SH3 exhibited a relatively high extraction yield, the lowest levels of impurities, and was the most effective at reversing the 6-OHDA-induced neurotoxicity of SH-SY5Y cells among SH1-SH3, which taken together indicate that it may have potential as a candidate therapeutic agent for the preventive therapy of neurodegenerative diseases.
29286349	8	9	theme	candidate	1565:1573	arg1	potential					1550:1558	potential	1550:1558	potential	1550:1558	SH3 exhibited a relatively high extraction yield, the lowest levels of impurities, and was the most effective at reversing the 6-OHDA-induced neurotoxicity of SH-SY5Y cells among SH1-SH3, which taken together indicate that it may have potential as a candidate therapeutic agent for the preventive therapy of neurodegenerative diseases.
29286349	8	10	theme	impurities	1386:1395	arg1	yield					1358:1362	a relatively high extraction yield	1329:1362	a relatively high extraction yield	1329:1362	SH3 exhibited a relatively high extraction yield, the lowest levels of impurities, and was the most effective at reversing the 6-OHDA-induced neurotoxicity of SH-SY5Y cells among SH1-SH3, which taken together indicate that it may have potential as a candidate therapeutic agent for the preventive therapy of neurodegenerative diseases.
29286349	8	10	theme	impurities	1386:1395	arg1	levels					1376:1381	the lowest levels	1365:1381	the lowest levels of impurities	1365:1395	SH3 exhibited a relatively high extraction yield, the lowest levels of impurities, and was the most effective at reversing the 6-OHDA-induced neurotoxicity of SH-SY5Y cells among SH1-SH3, which taken together indicate that it may have potential as a candidate therapeutic agent for the preventive therapy of neurodegenerative diseases.
29286349	4	11	theme	weight	886:891	arg1	analyses					810:817	the analyses	806:817	the analyses of composition, FTIR spectroscopy, NMR spectroscopy, and molecular weight	806:891	The SH1-SH3 extracts exhibited characteristics of fucoidan as demonstrated by the analyses of composition, FTIR spectroscopy, NMR spectroscopy, and molecular weight.
29286349	4	12	theme	composition	822:832	arg1	analyses					810:817	the analyses	806:817	the analyses of composition, FTIR spectroscopy, NMR spectroscopy, and molecular weight	806:891	The SH1-SH3 extracts exhibited characteristics of fucoidan as demonstrated by the analyses of composition, FTIR spectroscopy, NMR spectroscopy, and molecular weight.
29286349	3	13	theme	fucoidan	660:667	arg1	sulfate/fucose					642:655	sulfate/fucose	642:655	sulfate/fucose of fucoidan	642:667	The results indicate that CPP increased the extraction yield, total sugar content, and molar ratios of sulfate/fucose of fucoidan and decreased molecular weight and impurities of fucoidan.
29286349	5	14	theme	antioxidant	922:932	arg1	activities					934:943	antioxidant activities	922:943	antioxidant activities	922:943	All SH1-SH3 extracts showed antioxidant activities.
29286349	8	15	theme	neurodegenerative	1623:1639	arg1	diseases					1641:1648	neurodegenerative diseases	1623:1648	neurodegenerative diseases	1623:1648	SH3 exhibited a relatively high extraction yield, the lowest levels of impurities, and was the most effective at reversing the 6-OHDA-induced neurotoxicity of SH-SY5Y cells among SH1-SH3, which taken together indicate that it may have potential as a candidate therapeutic agent for the preventive therapy of neurodegenerative diseases.
29286349	0	16	theme	6-Hydroxydopamine-Induced	129:153	arg1	Apoptosis					155:163	6-Hydroxydopamine-Induced Apoptosis	129:163	6-Hydroxydopamine-Induced Apoptosis in SH-SY5Y Cells	129:180	Compressional-Puffing Pretreatment Enhances Neuroprotective Effects of Fucoidans from the Brown Seaweed Sargassum hemiphyllum on 6-Hydroxydopamine-Induced Apoptosis in SH-SY5Y Cells.
29286349	1	17	theme	compressional-puffing	200:220	arg1	process					222:228	a compressional-puffing process	198:228	a compressional-puffing process (CPP)	198:234	In this study, a compressional-puffing process (CPP) was used to pretreat Sargassum hemiphyllum (SH) and then fucoidan was extracted from SH by hot water.
29286349	1	17	theme	compressional-puffing	200:220	arg1	CPP					231:233	CPP	231:233	CPP	231:233	In this study, a compressional-puffing process (CPP) was used to pretreat Sargassum hemiphyllum (SH) and then fucoidan was extracted from SH by hot water.
29286349	0	18	from	Effects	60:66	arg1	hemiphyllum					114:124	the Brown Seaweed Sargassum hemiphyllum	86:124	the Brown Seaweed Sargassum hemiphyllum	86:124	Compressional-Puffing Pretreatment Enhances Neuroprotective Effects of Fucoidans from the Brown Seaweed Sargassum hemiphyllum on 6-Hydroxydopamine-Induced Apoptosis in SH-SY5Y Cells.
29286349	0	18	from	Effects	60:66	arg1	Apoptosis					155:163	6-Hydroxydopamine-Induced Apoptosis	129:163	6-Hydroxydopamine-Induced Apoptosis in SH-SY5Y Cells	129:180	Compressional-Puffing Pretreatment Enhances Neuroprotective Effects of Fucoidans from the Brown Seaweed Sargassum hemiphyllum on 6-Hydroxydopamine-Induced Apoptosis in SH-SY5Y Cells.
29286349	6	19	theme	caspase-8	1120:1128	arg1	distribution					1070:1081	cell cycle distribution	1059:1081	cell cycle distribution	1059:1081	The SH1-SH3 extracts protected SH-SY5Y cells from 6-hydroxydopamine (6-OHDA)-induced apoptosis as illustrated by cell cycle distribution, cytochrome c release, activation of caspase-8, -9, and -3, and DNA fragmentation analyses.
29286349	6	19	theme	caspase-8	1120:1128	arg1	activation					1106:1115	activation	1106:1115	activation of caspase-8, -9, and -3	1106:1140	The SH1-SH3 extracts protected SH-SY5Y cells from 6-hydroxydopamine (6-OHDA)-induced apoptosis as illustrated by cell cycle distribution, cytochrome c release, activation of caspase-8, -9, and -3, and DNA fragmentation analyses.
29286349	6	19	theme	caspase-8	1120:1128	arg1	fragmentation					1151:1163	DNA fragmentation	1147:1163	DNA fragmentation	1147:1163	The SH1-SH3 extracts protected SH-SY5Y cells from 6-hydroxydopamine (6-OHDA)-induced apoptosis as illustrated by cell cycle distribution, cytochrome c release, activation of caspase-8, -9, and -3, and DNA fragmentation analyses.
29286349	6	19	theme	caspase-8	1120:1128	arg1	release					1097:1103	cytochrome c release	1084:1103	cytochrome c release	1084:1103	The SH1-SH3 extracts protected SH-SY5Y cells from 6-hydroxydopamine (6-OHDA)-induced apoptosis as illustrated by cell cycle distribution, cytochrome c release, activation of caspase-8, -9, and -3, and DNA fragmentation analyses.
29286349	4	20	theme	FTIR	835:838	arg1	spectroscopy					840:851	FTIR spectroscopy	835:851	FTIR spectroscopy	835:851	The SH1-SH3 extracts exhibited characteristics of fucoidan as demonstrated by the analyses of composition, FTIR spectroscopy, NMR spectroscopy, and molecular weight.
29286349	0	21	theme	Compressional-Puffing	0:20	arg1	Pretreatment					22:33	Compressional-Puffing Pretreatment	0:33	Compressional-Puffing Pretreatment	0:33	Compressional-Puffing Pretreatment Enhances Neuroprotective Effects of Fucoidans from the Brown Seaweed Sargassum hemiphyllum on 6-Hydroxydopamine-Induced Apoptosis in SH-SY5Y Cells.
29286349	3	22	theme	molecular	683:691	arg1	weight					693:698	molecular weight	683:698	molecular weight	683:698	The results indicate that CPP increased the extraction yield, total sugar content, and molar ratios of sulfate/fucose of fucoidan and decreased molecular weight and impurities of fucoidan.
29286349	3	23	theme	total	601:605	arg1	content					613:619	total sugar content	601:619	total sugar content	601:619	The results indicate that CPP increased the extraction yield, total sugar content, and molar ratios of sulfate/fucose of fucoidan and decreased molecular weight and impurities of fucoidan.
29286349	0	24	from	Apoptosis	155:163	arg1	Cells					176:180	SH-SY5Y Cells	168:180	SH-SY5Y Cells	168:180	Compressional-Puffing Pretreatment Enhances Neuroprotective Effects of Fucoidans from the Brown Seaweed Sargassum hemiphyllum on 6-Hydroxydopamine-Induced Apoptosis in SH-SY5Y Cells.
29286349	6	25	theme	-induced	1022:1029	arg1	apoptosis					1031:1039	6-hydroxydopamine (6-OHDA)-induced apoptosis	996:1039	6-hydroxydopamine (6-OHDA)-induced apoptosis	996:1039	The SH1-SH3 extracts protected SH-SY5Y cells from 6-hydroxydopamine (6-OHDA)-induced apoptosis as illustrated by cell cycle distribution, cytochrome c release, activation of caspase-8, -9, and -3, and DNA fragmentation analyses.
29286349	0	26	theme	Neuroprotective	44:58	arg1	Effects					60:66	Neuroprotective Effects	44:66	Neuroprotective Effects of Fucoidans from the Brown Seaweed Sargassum hemiphyllum on 6-Hydroxydopamine-Induced Apoptosis in SH-SY5Y Cells	44:180	Compressional-Puffing Pretreatment Enhances Neuroprotective Effects of Fucoidans from the Brown Seaweed Sargassum hemiphyllum on 6-Hydroxydopamine-Induced Apoptosis in SH-SY5Y Cells.
29286349	0	27	theme	SH-SY5Y	168:174	arg1	Cells					176:180	SH-SY5Y Cells	168:180	SH-SY5Y Cells	168:180	Compressional-Puffing Pretreatment Enhances Neuroprotective Effects of Fucoidans from the Brown Seaweed Sargassum hemiphyllum on 6-Hydroxydopamine-Induced Apoptosis in SH-SY5Y Cells.
29286349	6	28	theme	activation	1106:1115	arg1	analyses					1165:1172	cell cycle distribution, cytochrome c release, activation of caspase-8, -9, and -3, and DNA fragmentation analyses	1059:1172	cell cycle distribution, cytochrome c release, activation of caspase-8, -9, and -3, and DNA fragmentation analyses	1059:1172	The SH1-SH3 extracts protected SH-SY5Y cells from 6-hydroxydopamine (6-OHDA)-induced apoptosis as illustrated by cell cycle distribution, cytochrome c release, activation of caspase-8, -9, and -3, and DNA fragmentation analyses.
29286349	7	29	theme	opposing	1254:1261	arg1	effects					1263:1269	the opposing effects	1250:1269	the opposing effects of SH1-SH3 on 6-OHDA-induced neurotoxicity	1250:1312	Additional experiments revealed that phosphorylation of Akt is involved in the opposing effects of SH1-SH3 on 6-OHDA-induced neurotoxicity.
29286349	8	30	theme	cells	1482:1486	arg1	neurotoxicity					1457:1469	the 6-OHDA-induced neurotoxicity	1438:1469	the 6-OHDA-induced neurotoxicity of SH-SY5Y cells	1438:1486	SH3 exhibited a relatively high extraction yield, the lowest levels of impurities, and was the most effective at reversing the 6-OHDA-induced neurotoxicity of SH-SY5Y cells among SH1-SH3, which taken together indicate that it may have potential as a candidate therapeutic agent for the preventive therapy of neurodegenerative diseases.
29286349	4	31	theme	spectroscopy	858:869	arg1	analyses					810:817	the analyses	806:817	the analyses of composition, FTIR spectroscopy, NMR spectroscopy, and molecular weight	806:891	The SH1-SH3 extracts exhibited characteristics of fucoidan as demonstrated by the analyses of composition, FTIR spectroscopy, NMR spectroscopy, and molecular weight.
29286349	3	32	theme	extraction	583:592	arg1	yield					594:598	the extraction yield	579:598	the extraction yield	579:598	The results indicate that CPP increased the extraction yield, total sugar content, and molar ratios of sulfate/fucose of fucoidan and decreased molecular weight and impurities of fucoidan.
29286349	5	33	theme	SH1-SH3	898:904	arg1	extracts					906:913	All SH1-SH3 extracts	894:913	All SH1-SH3 extracts	894:913	All SH1-SH3 extracts showed antioxidant activities.
29286349	4	34	theme	molecular	876:884	arg1	weight					886:891	molecular weight	876:891	molecular weight	876:891	The SH1-SH3 extracts exhibited characteristics of fucoidan as demonstrated by the analyses of composition, FTIR spectroscopy, NMR spectroscopy, and molecular weight.
29286349	8	35	theme	preventive	1601:1610	arg1	therapy					1612:1618	the preventive therapy	1597:1618	the preventive therapy of neurodegenerative diseases	1597:1648	SH3 exhibited a relatively high extraction yield, the lowest levels of impurities, and was the most effective at reversing the 6-OHDA-induced neurotoxicity of SH-SY5Y cells among SH1-SH3, which taken together indicate that it may have potential as a candidate therapeutic agent for the preventive therapy of neurodegenerative diseases.
29286349	1	36	used	used	240:243	arg2	CPP					231:233	CPP	231:233	CPP	231:233	In this study, a compressional-puffing process (CPP) was used to pretreat Sargassum hemiphyllum (SH) and then fucoidan was extracted from SH by hot water.
29286349	1	36	used	used	240:243	arg2	process					222:228	a compressional-puffing process	198:228	a compressional-puffing process (CPP)	198:234	In this study, a compressional-puffing process (CPP) was used to pretreat Sargassum hemiphyllum (SH) and then fucoidan was extracted from SH by hot water.
29286349	6	37	theme	-3	1139:1140	arg1	distribution					1070:1081	cell cycle distribution	1059:1081	cell cycle distribution	1059:1081	The SH1-SH3 extracts protected SH-SY5Y cells from 6-hydroxydopamine (6-OHDA)-induced apoptosis as illustrated by cell cycle distribution, cytochrome c release, activation of caspase-8, -9, and -3, and DNA fragmentation analyses.
29286349	6	37	theme	-3	1139:1140	arg1	activation					1106:1115	activation	1106:1115	activation of caspase-8, -9, and -3	1106:1140	The SH1-SH3 extracts protected SH-SY5Y cells from 6-hydroxydopamine (6-OHDA)-induced apoptosis as illustrated by cell cycle distribution, cytochrome c release, activation of caspase-8, -9, and -3, and DNA fragmentation analyses.
29286349	6	37	theme	-3	1139:1140	arg1	fragmentation					1151:1163	DNA fragmentation	1147:1163	DNA fragmentation	1147:1163	The SH1-SH3 extracts protected SH-SY5Y cells from 6-hydroxydopamine (6-OHDA)-induced apoptosis as illustrated by cell cycle distribution, cytochrome c release, activation of caspase-8, -9, and -3, and DNA fragmentation analyses.
29286349	6	37	theme	-3	1139:1140	arg1	release					1097:1103	cytochrome c release	1084:1103	cytochrome c release	1084:1103	The SH1-SH3 extracts protected SH-SY5Y cells from 6-hydroxydopamine (6-OHDA)-induced apoptosis as illustrated by cell cycle distribution, cytochrome c release, activation of caspase-8, -9, and -3, and DNA fragmentation analyses.
29286349	8	38	theme	therapeutic	1575:1585	arg1	agent					1587:1591	a candidate therapeutic agent	1563:1591	a candidate therapeutic agent for the preventive therapy of neurodegenerative diseases	1563:1648	SH3 exhibited a relatively high extraction yield, the lowest levels of impurities, and was the most effective at reversing the 6-OHDA-induced neurotoxicity of SH-SY5Y cells among SH1-SH3, which taken together indicate that it may have potential as a candidate therapeutic agent for the preventive therapy of neurodegenerative diseases.
29286349	8	38	theme	therapeutic	1575:1585	arg1	potential					1550:1558	potential	1550:1558	potential	1550:1558	SH3 exhibited a relatively high extraction yield, the lowest levels of impurities, and was the most effective at reversing the 6-OHDA-induced neurotoxicity of SH-SY5Y cells among SH1-SH3, which taken together indicate that it may have potential as a candidate therapeutic agent for the preventive therapy of neurodegenerative diseases.
29286349	6	39	theme	SH-SY5Y	977:983	arg1	cells					985:989	SH-SY5Y cells	977:989	SH-SY5Y cells	977:989	The SH1-SH3 extracts protected SH-SY5Y cells from 6-hydroxydopamine (6-OHDA)-induced apoptosis as illustrated by cell cycle distribution, cytochrome c release, activation of caspase-8, -9, and -3, and DNA fragmentation analyses.
29286349	3	40	theme	fucoidan	718:725	arg1	impurities					704:713	impurities	704:713	impurities of fucoidan	704:725	The results indicate that CPP increased the extraction yield, total sugar content, and molar ratios of sulfate/fucose of fucoidan and decreased molecular weight and impurities of fucoidan.
29286349	3	40	theme	fucoidan	718:725	arg1	weight					693:698	molecular weight	683:698	molecular weight	683:698	The results indicate that CPP increased the extraction yield, total sugar content, and molar ratios of sulfate/fucose of fucoidan and decreased molecular weight and impurities of fucoidan.
29286349	4	41	theme	spectroscopy	840:851	arg1	analyses					810:817	the analyses	806:817	the analyses of composition, FTIR spectroscopy, NMR spectroscopy, and molecular weight	806:891	The SH1-SH3 extracts exhibited characteristics of fucoidan as demonstrated by the analyses of composition, FTIR spectroscopy, NMR spectroscopy, and molecular weight.
29286349	4	42	theme	fucoidan	778:785	arg1	characteristics					759:773	characteristics	759:773	characteristics of fucoidan	759:785	The SH1-SH3 extracts exhibited characteristics of fucoidan as demonstrated by the analyses of composition, FTIR spectroscopy, NMR spectroscopy, and molecular weight.
29286349	8	43	contain	have	1545:1548	arg1	it					1538:1539	it	1538:1539	it	1538:1539	SH3 exhibited a relatively high extraction yield, the lowest levels of impurities, and was the most effective at reversing the 6-OHDA-induced neurotoxicity of SH-SY5Y cells among SH1-SH3, which taken together indicate that it may have potential as a candidate therapeutic agent for the preventive therapy of neurodegenerative diseases.
29286349	8	43	contain	have	1545:1548	arg2	agent					1587:1591	a candidate therapeutic agent	1563:1591	a candidate therapeutic agent for the preventive therapy of neurodegenerative diseases	1563:1648	SH3 exhibited a relatively high extraction yield, the lowest levels of impurities, and was the most effective at reversing the 6-OHDA-induced neurotoxicity of SH-SY5Y cells among SH1-SH3, which taken together indicate that it may have potential as a candidate therapeutic agent for the preventive therapy of neurodegenerative diseases.
29286349	8	43	contain	have	1545:1548	arg2	potential					1550:1558	potential	1550:1558	potential	1550:1558	SH3 exhibited a relatively high extraction yield, the lowest levels of impurities, and was the most effective at reversing the 6-OHDA-induced neurotoxicity of SH-SY5Y cells among SH1-SH3, which taken together indicate that it may have potential as a candidate therapeutic agent for the preventive therapy of neurodegenerative diseases.
29286349	2	44	theme	fucoidan	344:351	arg1	SH3					430:432	SH3	430:432	SH3 (puffing at 10.0 kg/cm²)	430:457	Three fucoidan extracts, namely SH1 (puffing at 0 kg/cm²); SH2 (puffing at 1.7 kg/cm²); and SH3 (puffing at 10.0 kg/cm²) were obtained, and their compositions and biological activities were evaluated.
29286349	2	44	theme	fucoidan	344:351	arg1	SH2					397:399	SH2	397:399	SH2 (puffing at 1.7 kg/cm²)	397:423	Three fucoidan extracts, namely SH1 (puffing at 0 kg/cm²); SH2 (puffing at 1.7 kg/cm²); and SH3 (puffing at 10.0 kg/cm²) were obtained, and their compositions and biological activities were evaluated.
29286349	2	44	theme	fucoidan	344:351	arg1	extracts					353:360	Three fucoidan extracts	338:360	Three fucoidan extracts	338:360	Three fucoidan extracts, namely SH1 (puffing at 0 kg/cm²); SH2 (puffing at 1.7 kg/cm²); and SH3 (puffing at 10.0 kg/cm²) were obtained, and their compositions and biological activities were evaluated.
29286349	2	44	theme	fucoidan	344:351	arg1	SH1					370:372	SH1	370:372	SH1 (puffing at 0 kg/cm²)	370:394	Three fucoidan extracts, namely SH1 (puffing at 0 kg/cm²); SH2 (puffing at 1.7 kg/cm²); and SH3 (puffing at 10.0 kg/cm²) were obtained, and their compositions and biological activities were evaluated.
29286349	0	45	theme	Fucoidans	71:79	arg1	Effects					60:66	Neuroprotective Effects	44:66	Neuroprotective Effects of Fucoidans from the Brown Seaweed Sargassum hemiphyllum on 6-Hydroxydopamine-Induced Apoptosis in SH-SY5Y Cells	44:180	Compressional-Puffing Pretreatment Enhances Neuroprotective Effects of Fucoidans from the Brown Seaweed Sargassum hemiphyllum on 6-Hydroxydopamine-Induced Apoptosis in SH-SY5Y Cells.
29286349	8	46	theme	lowest	1369:1374	arg1	yield					1358:1362	a relatively high extraction yield	1329:1362	a relatively high extraction yield	1329:1362	SH3 exhibited a relatively high extraction yield, the lowest levels of impurities, and was the most effective at reversing the 6-OHDA-induced neurotoxicity of SH-SY5Y cells among SH1-SH3, which taken together indicate that it may have potential as a candidate therapeutic agent for the preventive therapy of neurodegenerative diseases.
29286349	8	46	theme	lowest	1369:1374	arg1	levels					1376:1381	the lowest levels	1365:1381	the lowest levels of impurities	1365:1395	SH3 exhibited a relatively high extraction yield, the lowest levels of impurities, and was the most effective at reversing the 6-OHDA-induced neurotoxicity of SH-SY5Y cells among SH1-SH3, which taken together indicate that it may have potential as a candidate therapeutic agent for the preventive therapy of neurodegenerative diseases.
29286349	2	47	theme	biological	501:510	arg1	activities					512:521	biological activities	501:521	biological activities	501:521	Three fucoidan extracts, namely SH1 (puffing at 0 kg/cm²); SH2 (puffing at 1.7 kg/cm²); and SH3 (puffing at 10.0 kg/cm²) were obtained, and their compositions and biological activities were evaluated.
29286349	3	48	theme	sugar	607:611	arg1	content					613:619	total sugar content	601:619	total sugar content	601:619	The results indicate that CPP increased the extraction yield, total sugar content, and molar ratios of sulfate/fucose of fucoidan and decreased molecular weight and impurities of fucoidan.
29286349	6	49	theme	fragmentation	1151:1163	arg1	analyses					1165:1172	cell cycle distribution, cytochrome c release, activation of caspase-8, -9, and -3, and DNA fragmentation analyses	1059:1172	cell cycle distribution, cytochrome c release, activation of caspase-8, -9, and -3, and DNA fragmentation analyses	1059:1172	The SH1-SH3 extracts protected SH-SY5Y cells from 6-hydroxydopamine (6-OHDA)-induced apoptosis as illustrated by cell cycle distribution, cytochrome c release, activation of caspase-8, -9, and -3, and DNA fragmentation analyses.
29286349	7	50	theme	6-OHDA-induced	1285:1298	arg1	neurotoxicity					1300:1312	6-OHDA-induced neurotoxicity	1285:1312	6-OHDA-induced neurotoxicity	1285:1312	Additional experiments revealed that phosphorylation of Akt is involved in the opposing effects of SH1-SH3 on 6-OHDA-induced neurotoxicity.
29286349	6	51	theme	c	1095:1095	arg1	release					1097:1103	cytochrome c release	1084:1103	cytochrome c release	1084:1103	The SH1-SH3 extracts protected SH-SY5Y cells from 6-hydroxydopamine (6-OHDA)-induced apoptosis as illustrated by cell cycle distribution, cytochrome c release, activation of caspase-8, -9, and -3, and DNA fragmentation analyses.
29286349	1	52	theme	hot	327:329	arg1	water					331:335	hot water	327:335	hot water	327:335	In this study, a compressional-puffing process (CPP) was used to pretreat Sargassum hemiphyllum (SH) and then fucoidan was extracted from SH by hot water.
29286349	4	53	theme	NMR	854:856	arg1	spectroscopy					858:869	NMR spectroscopy	854:869	NMR spectroscopy	854:869	The SH1-SH3 extracts exhibited characteristics of fucoidan as demonstrated by the analyses of composition, FTIR spectroscopy, NMR spectroscopy, and molecular weight.
29286349	6	54	theme	DNA	1147:1149	arg1	fragmentation					1151:1163	DNA fragmentation	1147:1163	DNA fragmentation	1147:1163	The SH1-SH3 extracts protected SH-SY5Y cells from 6-hydroxydopamine (6-OHDA)-induced apoptosis as illustrated by cell cycle distribution, cytochrome c release, activation of caspase-8, -9, and -3, and DNA fragmentation analyses.
29286349	7	55	from	effects	1263:1269	arg1	neurotoxicity					1300:1312	6-OHDA-induced neurotoxicity	1285:1312	6-OHDA-induced neurotoxicity	1285:1312	Additional experiments revealed that phosphorylation of Akt is involved in the opposing effects of SH1-SH3 on 6-OHDA-induced neurotoxicity.
29286349	6	56	theme	cytochrome	1084:1093	arg1	release					1097:1103	cytochrome c release	1084:1103	cytochrome c release	1084:1103	The SH1-SH3 extracts protected SH-SY5Y cells from 6-hydroxydopamine (6-OHDA)-induced apoptosis as illustrated by cell cycle distribution, cytochrome c release, activation of caspase-8, -9, and -3, and DNA fragmentation analyses.
29286349	8	57	theme	extraction	1347:1356	arg1	yield					1358:1362	a relatively high extraction yield	1329:1362	a relatively high extraction yield	1329:1362	SH3 exhibited a relatively high extraction yield, the lowest levels of impurities, and was the most effective at reversing the 6-OHDA-induced neurotoxicity of SH-SY5Y cells among SH1-SH3, which taken together indicate that it may have potential as a candidate therapeutic agent for the preventive therapy of neurodegenerative diseases.
29286349	8	57	theme	extraction	1347:1356	arg1	levels					1376:1381	the lowest levels	1365:1381	the lowest levels of impurities	1365:1395	SH3 exhibited a relatively high extraction yield, the lowest levels of impurities, and was the most effective at reversing the 6-OHDA-induced neurotoxicity of SH-SY5Y cells among SH1-SH3, which taken together indicate that it may have potential as a candidate therapeutic agent for the preventive therapy of neurodegenerative diseases.
29286349	8	58	theme	6-OHDA-induced	1442:1455	arg1	neurotoxicity					1457:1469	the 6-OHDA-induced neurotoxicity	1438:1469	the 6-OHDA-induced neurotoxicity of SH-SY5Y cells	1438:1486	SH3 exhibited a relatively high extraction yield, the lowest levels of impurities, and was the most effective at reversing the 6-OHDA-induced neurotoxicity of SH-SY5Y cells among SH1-SH3, which taken together indicate that it may have potential as a candidate therapeutic agent for the preventive therapy of neurodegenerative diseases.
29286349	7	59	theme	Akt	1231:1233	arg1	phosphorylation					1212:1226	phosphorylation	1212:1226	phosphorylation of Akt	1212:1233	Additional experiments revealed that phosphorylation of Akt is involved in the opposing effects of SH1-SH3 on 6-OHDA-induced neurotoxicity.
29286349	6	60	theme	release	1097:1103	arg1	analyses					1165:1172	cell cycle distribution, cytochrome c release, activation of caspase-8, -9, and -3, and DNA fragmentation analyses	1059:1172	cell cycle distribution, cytochrome c release, activation of caspase-8, -9, and -3, and DNA fragmentation analyses	1059:1172	The SH1-SH3 extracts protected SH-SY5Y cells from 6-hydroxydopamine (6-OHDA)-induced apoptosis as illustrated by cell cycle distribution, cytochrome c release, activation of caspase-8, -9, and -3, and DNA fragmentation analyses.
29286349	1	61	theme	pretreat	248:255	arg1	SH					280:281	SH	280:281	SH	280:281	In this study, a compressional-puffing process (CPP) was used to pretreat Sargassum hemiphyllum (SH) and then fucoidan was extracted from SH by hot water.
29286349	1	61	theme	pretreat	248:255	arg1	hemiphyllum					267:277	pretreat Sargassum hemiphyllum	248:277	pretreat Sargassum hemiphyllum (SH)	248:282	In this study, a compressional-puffing process (CPP) was used to pretreat Sargassum hemiphyllum (SH) and then fucoidan was extracted from SH by hot water.
29286349	0	62	theme	Brown	90:94	arg1	hemiphyllum					114:124	the Brown Seaweed Sargassum hemiphyllum	86:124	the Brown Seaweed Sargassum hemiphyllum	86:124	Compressional-Puffing Pretreatment Enhances Neuroprotective Effects of Fucoidans from the Brown Seaweed Sargassum hemiphyllum on 6-Hydroxydopamine-Induced Apoptosis in SH-SY5Y Cells.
29286349	0	63	from	hemiphyllum	114:124	arg1	Fucoidans					71:79	Fucoidans	71:79	Fucoidans from the Brown Seaweed Sargassum hemiphyllum	71:124	Compressional-Puffing Pretreatment Enhances Neuroprotective Effects of Fucoidans from the Brown Seaweed Sargassum hemiphyllum on 6-Hydroxydopamine-Induced Apoptosis in SH-SY5Y Cells.
29286349	0	63	from	hemiphyllum	114:124	arg1	Effects					60:66	Neuroprotective Effects	44:66	Neuroprotective Effects of Fucoidans from the Brown Seaweed Sargassum hemiphyllum on 6-Hydroxydopamine-Induced Apoptosis in SH-SY5Y Cells	44:180	Compressional-Puffing Pretreatment Enhances Neuroprotective Effects of Fucoidans from the Brown Seaweed Sargassum hemiphyllum on 6-Hydroxydopamine-Induced Apoptosis in SH-SY5Y Cells.
29286349	6	64	theme	distribution	1070:1081	arg1	analyses					1165:1172	cell cycle distribution, cytochrome c release, activation of caspase-8, -9, and -3, and DNA fragmentation analyses	1059:1172	cell cycle distribution, cytochrome c release, activation of caspase-8, -9, and -3, and DNA fragmentation analyses	1059:1172	The SH1-SH3 extracts protected SH-SY5Y cells from 6-hydroxydopamine (6-OHDA)-induced apoptosis as illustrated by cell cycle distribution, cytochrome c release, activation of caspase-8, -9, and -3, and DNA fragmentation analyses.
29286349	6	65	theme	-9	1131:1132	arg1	distribution					1070:1081	cell cycle distribution	1059:1081	cell cycle distribution	1059:1081	The SH1-SH3 extracts protected SH-SY5Y cells from 6-hydroxydopamine (6-OHDA)-induced apoptosis as illustrated by cell cycle distribution, cytochrome c release, activation of caspase-8, -9, and -3, and DNA fragmentation analyses.
29286349	6	65	theme	-9	1131:1132	arg1	activation					1106:1115	activation	1106:1115	activation of caspase-8, -9, and -3	1106:1140	The SH1-SH3 extracts protected SH-SY5Y cells from 6-hydroxydopamine (6-OHDA)-induced apoptosis as illustrated by cell cycle distribution, cytochrome c release, activation of caspase-8, -9, and -3, and DNA fragmentation analyses.
29286349	6	65	theme	-9	1131:1132	arg1	fragmentation					1151:1163	DNA fragmentation	1147:1163	DNA fragmentation	1147:1163	The SH1-SH3 extracts protected SH-SY5Y cells from 6-hydroxydopamine (6-OHDA)-induced apoptosis as illustrated by cell cycle distribution, cytochrome c release, activation of caspase-8, -9, and -3, and DNA fragmentation analyses.
29286349	6	65	theme	-9	1131:1132	arg1	release					1097:1103	cytochrome c release	1084:1103	cytochrome c release	1084:1103	The SH1-SH3 extracts protected SH-SY5Y cells from 6-hydroxydopamine (6-OHDA)-induced apoptosis as illustrated by cell cycle distribution, cytochrome c release, activation of caspase-8, -9, and -3, and DNA fragmentation analyses.
29286349	1	66	theme	Sargassum	257:265	arg1	SH					280:281	SH	280:281	SH	280:281	In this study, a compressional-puffing process (CPP) was used to pretreat Sargassum hemiphyllum (SH) and then fucoidan was extracted from SH by hot water.
29286349	1	66	theme	Sargassum	257:265	arg1	hemiphyllum					267:277	pretreat Sargassum hemiphyllum	248:277	pretreat Sargassum hemiphyllum (SH)	248:282	In this study, a compressional-puffing process (CPP) was used to pretreat Sargassum hemiphyllum (SH) and then fucoidan was extracted from SH by hot water.
29286349	7	67	theme	Additional	1175:1184	arg1	experiments					1186:1196	Additional experiments	1175:1196	Additional experiments	1175:1196	Additional experiments revealed that phosphorylation of Akt is involved in the opposing effects of SH1-SH3 on 6-OHDA-induced neurotoxicity.
29286349	3	68	theme	molar	626:630	arg1	ratios					632:637	molar ratios	626:637	molar ratios	626:637	The results indicate that CPP increased the extraction yield, total sugar content, and molar ratios of sulfate/fucose of fucoidan and decreased molecular weight and impurities of fucoidan.
29286349	4	69	theme	SH1-SH3	732:738	arg1	extracts					740:747	The SH1-SH3 extracts	728:747	The SH1-SH3 extracts	728:747	The SH1-SH3 extracts exhibited characteristics of fucoidan as demonstrated by the analyses of composition, FTIR spectroscopy, NMR spectroscopy, and molecular weight.
29286349	6	70	theme	cycle	1064:1068	arg1	distribution					1070:1081	cell cycle distribution	1059:1081	cell cycle distribution	1059:1081	The SH1-SH3 extracts protected SH-SY5Y cells from 6-hydroxydopamine (6-OHDA)-induced apoptosis as illustrated by cell cycle distribution, cytochrome c release, activation of caspase-8, -9, and -3, and DNA fragmentation analyses.
27325464	7	0	theme	low	1691:1693	arg1	heparin					1712:1718	a low molecular weight heparin	1689:1718	a low molecular weight heparin	1689:1718	Here we demonstrate the analysis of heparin oligosaccharides, including disaccharides, ultralow molecular weight heparin, and a low molecular weight heparin, using a novel electrokinetic pump-based CE-MS coupling eletrospray ion source.
27325464	4	1	theme	heparin	1018:1024	arg1	analysis					985:992	bottom-up and top-down analysis	962:992	analysis	985:992	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	4	1	theme	heparin	1018:1024	arg1	analysis					949:956	disaccharide compositional analysis	922:956	disaccharide compositional analysis	922:956	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	8	2	theme	electrospray	1849:1860	arg1	ionization					1862:1871	negative-mode electrospray ionization	1835:1871	negative-mode electrospray ionization	1835:1871	Reverse polarity CE separation and negative-mode electrospray ionization were optimized using a volatile methanolic ammonium acetate electrolyte and sheath fluid.
27325464	9	3	from	analysis	2106:2113	arg1	useful					2069:2074	useful	2069:2074	useful	2069:2074	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	9	3	from	analysis	2106:2113	arg1	MS					2025:2026	The online CE hyphenated negative-ion electrospray ionization MS	1963:2026	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer	1963:2063	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	6	4	theme	liquid	1330:1335	arg1	chromatography-MS					1337:1353	liquid chromatography-MS	1330:1353	liquid chromatography-MS	1330:1353	Graphical abstract Most hyphenated analytical approaches that rely on liquid chromatography-MS require relatively long separation times, produce incomplete resolution of oligosaccharide mixtures, use eluents that are incompatible with electrospray ionization, or require oligosaccharide derivatization.
27325464	9	5	theme	molecular	2158:2166	arg1	heparin					2175:2181	low molecular weight heparin	2154:2181	low molecular weight heparin	2154:2181	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	10	6	theme	CE-MS	2208:2212	arg1	method					2214:2219	this CE-MS method	2203:2219	this CE-MS method	2203:2219	The application of this CE-MS method to ultralow molecular heparin suggests that a charge state distribution and the low level of sulfate group loss that is achieved make this method useful for online tandem MS analysis of heparins.
27325464	6	7	theme	mixtures	1446:1453	arg1	resolution					1416:1425	incomplete resolution	1405:1425	incomplete resolution of oligosaccharide mixtures	1405:1453	Graphical abstract Most hyphenated analytical approaches that rely on liquid chromatography-MS require relatively long separation times, produce incomplete resolution of oligosaccharide mixtures, use eluents that are incompatible with electrospray ionization, or require oligosaccharide derivatization.
27325464	3	8	theme	Reverse	643:649	arg1	separation					663:672	Reverse polarity CE separation	643:672	Reverse polarity CE separation	643:672	Reverse polarity CE separation and negative-mode electrospray ionization were optimized using a volatile methanolic ammonium acetate electrolyte and sheath fluid.
27325464	8	9	theme	sheath	1949:1954	arg1	fluid					1956:1960	sheath fluid	1949:1960	sheath fluid	1949:1960	Reverse polarity CE separation and negative-mode electrospray ionization were optimized using a volatile methanolic ammonium acetate electrolyte and sheath fluid.
27325464	9	10	from	useful	2069:2074	arg1	analysis					2142:2149	bottom-up and top-down analysis	2119:2149	analysis	2142:2149	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	9	10	from	useful	2069:2074	arg1	analysis					2106:2113	disaccharide compositional analysis	2079:2113	disaccharide compositional analysis	2079:2113	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	9	11	from	analysis	2142:2149	arg1	useful					2069:2074	useful	2069:2074	useful	2069:2074	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	9	11	from	analysis	2142:2149	arg1	MS					2025:2026	The online CE hyphenated negative-ion electrospray ionization MS	1963:2026	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer	1963:2063	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	9	12	theme	heparin	2175:2181	arg1	analysis					2142:2149	bottom-up and top-down analysis	2119:2149	analysis	2142:2149	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	9	12	theme	heparin	2175:2181	arg1	analysis					2106:2113	disaccharide compositional analysis	2079:2113	disaccharide compositional analysis	2079:2113	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	7	13	theme	molecular	1659:1667	arg1	heparin					1676:1682	ultralow molecular weight heparin	1650:1682	ultralow molecular weight heparin	1650:1682	Here we demonstrate the analysis of heparin oligosaccharides, including disaccharides, ultralow molecular weight heparin, and a low molecular weight heparin, using a novel electrokinetic pump-based CE-MS coupling eletrospray ion source.
27325464	4	14	theme	molecular	1001:1009	arg1	heparin					1018:1024	low molecular weight heparin	997:1024	low molecular weight heparin	997:1024	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	7	15	theme	heparin	1599:1605	arg1	heparin					1712:1718	a low molecular weight heparin	1689:1718	a low molecular weight heparin	1689:1718	Here we demonstrate the analysis of heparin oligosaccharides, including disaccharides, ultralow molecular weight heparin, and a low molecular weight heparin, using a novel electrokinetic pump-based CE-MS coupling eletrospray ion source.
27325464	7	15	theme	heparin	1599:1605	arg1	heparin					1676:1682	ultralow molecular weight heparin	1650:1682	ultralow molecular weight heparin	1650:1682	Here we demonstrate the analysis of heparin oligosaccharides, including disaccharides, ultralow molecular weight heparin, and a low molecular weight heparin, using a novel electrokinetic pump-based CE-MS coupling eletrospray ion source.
27325464	7	15	theme	heparin	1599:1605	arg1	oligosaccharides					1607:1622	heparin oligosaccharides	1599:1622	heparin oligosaccharides	1599:1622	Here we demonstrate the analysis of heparin oligosaccharides, including disaccharides, ultralow molecular weight heparin, and a low molecular weight heparin, using a novel electrokinetic pump-based CE-MS coupling eletrospray ion source.
27325464	7	15	theme	heparin	1599:1605	arg1	disaccharides					1635:1647	disaccharides	1635:1647	disaccharides	1635:1647	Here we demonstrate the analysis of heparin oligosaccharides, including disaccharides, ultralow molecular weight heparin, and a low molecular weight heparin, using a novel electrokinetic pump-based CE-MS coupling eletrospray ion source.
27325464	10	16	theme	molecular	2233:2241	arg1	heparin					2243:2249	ultralow molecular heparin	2224:2249	ultralow molecular heparin	2224:2249	The application of this CE-MS method to ultralow molecular heparin suggests that a charge state distribution and the low level of sulfate group loss that is achieved make this method useful for online tandem MS analysis of heparins.
27325464	6	17	theme	incomplete	1405:1414	arg1	resolution					1416:1425	incomplete resolution	1405:1425	incomplete resolution of oligosaccharide mixtures	1405:1453	Graphical abstract Most hyphenated analytical approaches that rely on liquid chromatography-MS require relatively long separation times, produce incomplete resolution of oligosaccharide mixtures, use eluents that are incompatible with electrospray ionization, or require oligosaccharide derivatization.
27325464	6	18	theme	hyphenated	1284:1293	arg1	approaches					1306:1315	Graphical abstract Most hyphenated analytical approaches	1260:1315	Graphical abstract Most hyphenated analytical approaches that rely on liquid chromatography-MS	1260:1353	Graphical abstract Most hyphenated analytical approaches that rely on liquid chromatography-MS require relatively long separation times, produce incomplete resolution of oligosaccharide mixtures, use eluents that are incompatible with electrospray ionization, or require oligosaccharide derivatization.
27325464	3	19	theme	CE	660:661	arg1	separation					663:672	Reverse polarity CE separation	643:672	Reverse polarity CE separation	643:672	Reverse polarity CE separation and negative-mode electrospray ionization were optimized using a volatile methanolic ammonium acetate electrolyte and sheath fluid.
27325464	10	20	theme	tandem	2385:2390	arg1	analysis					2395:2402	online tandem MS analysis	2378:2402	online tandem MS analysis of heparins	2378:2414	The application of this CE-MS method to ultralow molecular heparin suggests that a charge state distribution and the low level of sulfate group loss that is achieved make this method useful for online tandem MS analysis of heparins.
27325464	6	21	theme	electrospray	1495:1506	arg1	ionization					1508:1517	electrospray ionization	1495:1517	electrospray ionization	1495:1517	Graphical abstract Most hyphenated analytical approaches that rely on liquid chromatography-MS require relatively long separation times, produce incomplete resolution of oligosaccharide mixtures, use eluents that are incompatible with electrospray ionization, or require oligosaccharide derivatization.
27325464	1	22	theme	oligosaccharide	273:287	arg1	mixtures					289:296	oligosaccharide mixtures	273:296	oligosaccharide mixtures	273:296	Most hyphenated analytical approaches that rely on liquid chromatography-MS require relatively long separation times, produce incomplete resolution of oligosaccharide mixtures, use eluents that are incompatible with electrospray ionization, or require oligosaccharide derivatization.
27325464	8	23	dep	acetate	1925:1931	arg1	fluid					1956:1960	sheath fluid	1949:1960	sheath fluid	1949:1960	Reverse polarity CE separation and negative-mode electrospray ionization were optimized using a volatile methanolic ammonium acetate electrolyte and sheath fluid.
27325464	8	23	dep	acetate	1925:1931	arg1	electrolyte					1933:1943	electrolyte	1933:1943	electrolyte	1933:1943	Reverse polarity CE separation and negative-mode electrospray ionization were optimized using a volatile methanolic ammonium acetate electrolyte and sheath fluid.
27325464	4	24	theme	electrospray	844:855	arg1	useful					912:917	useful	912:917	useful	912:917	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	4	24	theme	electrospray	844:855	arg1	MS					868:869	The online CE hyphenated negative-ion electrospray ionization MS	806:869	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer	806:906	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	3	25	theme	electrospray	692:703	arg1	ionization					705:714	negative-mode electrospray ionization	678:714	negative-mode electrospray ionization	678:714	Reverse polarity CE separation and negative-mode electrospray ionization were optimized using a volatile methanolic ammonium acetate electrolyte and sheath fluid.
27325464	9	26	theme	CE	1974:1975	arg1	MS					2025:2026	The online CE hyphenated negative-ion electrospray ionization MS	1963:2026	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer	1963:2063	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	9	26	theme	CE	1974:1975	arg1	useful					2069:2074	useful	2069:2074	useful	2069:2074	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	6	27	theme	long	1374:1377	arg1	times					1390:1394	relatively long separation times	1363:1394	relatively long separation times	1363:1394	Graphical abstract Most hyphenated analytical approaches that rely on liquid chromatography-MS require relatively long separation times, produce incomplete resolution of oligosaccharide mixtures, use eluents that are incompatible with electrospray ionization, or require oligosaccharide derivatization.
27325464	10	28	theme	heparins	2407:2414	arg1	analysis					2395:2402	online tandem MS analysis	2378:2402	online tandem MS analysis of heparins	2378:2414	The application of this CE-MS method to ultralow molecular heparin suggests that a charge state distribution and the low level of sulfate group loss that is achieved make this method useful for online tandem MS analysis of heparins.
27325464	3	29	theme	volatile	739:746	arg1	acetate					768:774	a volatile methanolic ammonium acetate electrolyte and sheath fluid	737:803	a volatile methanolic ammonium acetate electrolyte and sheath fluid	737:803	Reverse polarity CE separation and negative-mode electrospray ionization were optimized using a volatile methanolic ammonium acetate electrolyte and sheath fluid.
27325464	4	30	from	analysis	949:956	arg1	MS					868:869	The online CE hyphenated negative-ion electrospray ionization MS	806:869	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer	806:906	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	4	30	from	analysis	949:956	arg1	useful					912:917	useful	912:917	useful	912:917	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	5	31	theme	molecular	1076:1084	arg1	heparin					1086:1092	ultralow molecular heparin	1067:1092	ultralow molecular heparin	1067:1092	The application of this CE-MS method to ultralow molecular heparin suggests that a charge state distribution and the low level of sulfate group loss that is achieved make this method useful for online tandem MS analysis of heparins.
27325464	2	32	theme	electrokinetic	578:591	arg1	source					635:640	a novel electrokinetic pump-based CE-MS coupling eletrospray ion source	570:640	a novel electrokinetic pump-based CE-MS coupling eletrospray ion source	570:640	Here we demonstrate the analysis of heparin oligosaccharides, including disaccharides, ultralow molecular weight heparin, and a low molecular weight heparin, using a novel electrokinetic pump-based CE-MS coupling eletrospray ion source.
27325464	9	33	theme	electrospray	2001:2012	arg1	MS					2025:2026	The online CE hyphenated negative-ion electrospray ionization MS	1963:2026	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer	1963:2063	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	9	33	theme	electrospray	2001:2012	arg1	useful					2069:2074	useful	2069:2074	useful	2069:2074	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	4	34	theme	online	810:815	arg1	useful					912:917	useful	912:917	useful	912:917	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	4	34	theme	online	810:815	arg1	MS					868:869	The online CE hyphenated negative-ion electrospray ionization MS	806:869	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer	806:906	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	10	35	theme	state	2274:2278	arg1	distribution					2280:2291	a charge state distribution	2265:2291	a charge state distribution	2265:2291	The application of this CE-MS method to ultralow molecular heparin suggests that a charge state distribution and the low level of sulfate group loss that is achieved make this method useful for online tandem MS analysis of heparins.
27325464	0	36	theme	ionization	92:101	arg1	spectrometry					108:119	capillary electrophoresis-negative-ion electrospray ionization mass spectrometry	40:119	capillary electrophoresis-negative-ion electrospray ionization mass spectrometry	40:119	Analysis of heparin oligosaccharides by capillary electrophoresis-negative-ion electrospray ionization mass spectrometry.
27325464	5	37	theme	heparins	1250:1257	arg1	analysis					1238:1245	online tandem MS analysis	1221:1245	online tandem MS analysis of heparins	1221:1257	The application of this CE-MS method to ultralow molecular heparin suggests that a charge state distribution and the low level of sulfate group loss that is achieved make this method useful for online tandem MS analysis of heparins.
27325464	9	38	theme	ionization	2014:2023	arg1	MS					2025:2026	The online CE hyphenated negative-ion electrospray ionization MS	1963:2026	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer	1963:2063	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	9	38	theme	ionization	2014:2023	arg1	useful					2069:2074	useful	2069:2074	useful	2069:2074	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	4	39	theme	hyphenated	820:829	arg1	useful					912:917	useful	912:917	useful	912:917	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	4	39	theme	hyphenated	820:829	arg1	MS					868:869	The online CE hyphenated negative-ion electrospray ionization MS	806:869	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer	806:906	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	4	40	from	useful	912:917	arg1	analysis					985:992	bottom-up and top-down analysis	962:992	analysis	985:992	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	4	40	from	useful	912:917	arg1	analysis					949:956	disaccharide compositional analysis	922:956	disaccharide compositional analysis	922:956	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	1	41	theme	long	217:220	arg1	times					233:237	relatively long separation times	206:237	relatively long separation times	206:237	Most hyphenated analytical approaches that rely on liquid chromatography-MS require relatively long separation times, produce incomplete resolution of oligosaccharide mixtures, use eluents that are incompatible with electrospray ionization, or require oligosaccharide derivatization.
27325464	7	42	theme	eletrospray	1776:1786	arg1	source					1792:1797	a novel electrokinetic pump-based CE-MS coupling eletrospray ion source	1727:1797	a novel electrokinetic pump-based CE-MS coupling eletrospray ion source	1727:1797	Here we demonstrate the analysis of heparin oligosaccharides, including disaccharides, ultralow molecular weight heparin, and a low molecular weight heparin, using a novel electrokinetic pump-based CE-MS coupling eletrospray ion source.
27325464	2	43	theme	eletrospray	619:629	arg1	source					635:640	a novel electrokinetic pump-based CE-MS coupling eletrospray ion source	570:640	a novel electrokinetic pump-based CE-MS coupling eletrospray ion source	570:640	Here we demonstrate the analysis of heparin oligosaccharides, including disaccharides, ultralow molecular weight heparin, and a low molecular weight heparin, using a novel electrokinetic pump-based CE-MS coupling eletrospray ion source.
27325464	10	44	theme	low	2301:2303	arg1	level					2305:2309	the low level	2297:2309	the low level of sulfate group loss that is achieved	2297:2348	The application of this CE-MS method to ultralow molecular heparin suggests that a charge state distribution and the low level of sulfate group loss that is achieved make this method useful for online tandem MS analysis of heparins.
27325464	5	45	theme	tandem	1228:1233	arg1	analysis					1238:1245	online tandem MS analysis	1221:1245	online tandem MS analysis of heparins	1221:1257	The application of this CE-MS method to ultralow molecular heparin suggests that a charge state distribution and the low level of sulfate group loss that is achieved make this method useful for online tandem MS analysis of heparins.
27325464	5	46	theme	state	1117:1121	arg1	distribution					1123:1134	a charge state distribution	1108:1134	a charge state distribution	1108:1134	The application of this CE-MS method to ultralow molecular heparin suggests that a charge state distribution and the low level of sulfate group loss that is achieved make this method useful for online tandem MS analysis of heparins.
27325464	1	47	theme	liquid	173:178	arg1	chromatography-MS					180:196	liquid chromatography-MS	173:196	liquid chromatography-MS	173:196	Most hyphenated analytical approaches that rely on liquid chromatography-MS require relatively long separation times, produce incomplete resolution of oligosaccharide mixtures, use eluents that are incompatible with electrospray ionization, or require oligosaccharide derivatization.
27325464	2	48	theme	CE-MS	604:608	arg1	source					635:640	a novel electrokinetic pump-based CE-MS coupling eletrospray ion source	570:640	a novel electrokinetic pump-based CE-MS coupling eletrospray ion source	570:640	Here we demonstrate the analysis of heparin oligosaccharides, including disaccharides, ultralow molecular weight heparin, and a low molecular weight heparin, using a novel electrokinetic pump-based CE-MS coupling eletrospray ion source.
27325464	10	49	theme	online	2378:2383	arg1	analysis					2395:2402	online tandem MS analysis	2378:2402	online tandem MS analysis of heparins	2378:2414	The application of this CE-MS method to ultralow molecular heparin suggests that a charge state distribution and the low level of sulfate group loss that is achieved make this method useful for online tandem MS analysis of heparins.
27325464	2	50	theme	low	534:536	arg1	heparin					555:561	a low molecular weight heparin	532:561	a low molecular weight heparin	532:561	Here we demonstrate the analysis of heparin oligosaccharides, including disaccharides, ultralow molecular weight heparin, and a low molecular weight heparin, using a novel electrokinetic pump-based CE-MS coupling eletrospray ion source.
27325464	9	51	theme	negative-ion	1988:1999	arg1	MS					2025:2026	The online CE hyphenated negative-ion electrospray ionization MS	1963:2026	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer	1963:2063	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	9	51	theme	negative-ion	1988:1999	arg1	useful					2069:2074	useful	2069:2074	useful	2069:2074	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	1	52	theme	electrospray	338:349	arg1	ionization					351:360	electrospray ionization	338:360	electrospray ionization	338:360	Most hyphenated analytical approaches that rely on liquid chromatography-MS require relatively long separation times, produce incomplete resolution of oligosaccharide mixtures, use eluents that are incompatible with electrospray ionization, or require oligosaccharide derivatization.
27325464	1	53	theme	hyphenated	127:136	arg1	approaches					149:158	Most hyphenated analytical approaches	122:158	Most hyphenated analytical approaches that rely on liquid chromatography-MS	122:196	Most hyphenated analytical approaches that rely on liquid chromatography-MS require relatively long separation times, produce incomplete resolution of oligosaccharide mixtures, use eluents that are incompatible with electrospray ionization, or require oligosaccharide derivatization.
27325464	4	54	theme	Orbitrap	881:888	arg1	spectrometer					895:906	an LTQ Orbitrap mass spectrometer	874:906	an LTQ Orbitrap mass spectrometer	874:906	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	0	55	theme	heparin	12:18	arg1	Analysis					0:7	Analysis	0:7	Analysis of heparin	0:18	Analysis of heparin oligosaccharides by capillary electrophoresis-negative-ion electrospray ionization mass spectrometry.
27325464	7	56	theme	novel	1729:1733	arg1	source					1792:1797	a novel electrokinetic pump-based CE-MS coupling eletrospray ion source	1727:1797	a novel electrokinetic pump-based CE-MS coupling eletrospray ion source	1727:1797	Here we demonstrate the analysis of heparin oligosaccharides, including disaccharides, ultralow molecular weight heparin, and a low molecular weight heparin, using a novel electrokinetic pump-based CE-MS coupling eletrospray ion source.
27325464	4	57	theme	bottom-up	962:970	arg1	analysis					985:992	bottom-up and top-down analysis	962:992	analysis	985:992	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	2	58	theme	molecular	502:510	arg1	heparin					519:525	ultralow molecular weight heparin	493:525	ultralow molecular weight heparin	493:525	Here we demonstrate the analysis of heparin oligosaccharides, including disaccharides, ultralow molecular weight heparin, and a low molecular weight heparin, using a novel electrokinetic pump-based CE-MS coupling eletrospray ion source.
27325464	5	59	theme	group	1165:1169	arg1	loss					1171:1174	sulfate group loss	1157:1174	sulfate group loss that is achieved	1157:1191	The application of this CE-MS method to ultralow molecular heparin suggests that a charge state distribution and the low level of sulfate group loss that is achieved make this method useful for online tandem MS analysis of heparins.
27325464	1	60	with	incompatible	320:331	arg1	ionization					351:360	electrospray ionization	338:360	electrospray ionization	338:360	Most hyphenated analytical approaches that rely on liquid chromatography-MS require relatively long separation times, produce incomplete resolution of oligosaccharide mixtures, use eluents that are incompatible with electrospray ionization, or require oligosaccharide derivatization.
27325464	2	61	theme	weight	548:553	arg1	heparin					555:561	a low molecular weight heparin	532:561	a low molecular weight heparin	532:561	Here we demonstrate the analysis of heparin oligosaccharides, including disaccharides, ultralow molecular weight heparin, and a low molecular weight heparin, using a novel electrokinetic pump-based CE-MS coupling eletrospray ion source.
27325464	3	62	theme	ammonium	759:766	arg1	acetate					768:774	a volatile methanolic ammonium acetate electrolyte and sheath fluid	737:803	a volatile methanolic ammonium acetate electrolyte and sheath fluid	737:803	Reverse polarity CE separation and negative-mode electrospray ionization were optimized using a volatile methanolic ammonium acetate electrolyte and sheath fluid.
27325464	4	63	theme	top-down	976:983	arg1	analysis					985:992	bottom-up and top-down analysis	962:992	analysis	985:992	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	0	64	theme	electrophoresis-negative-ion	50:77	arg1	spectrometry					108:119	capillary electrophoresis-negative-ion electrospray ionization mass spectrometry	40:119	capillary electrophoresis-negative-ion electrospray ionization mass spectrometry	40:119	Analysis of heparin oligosaccharides by capillary electrophoresis-negative-ion electrospray ionization mass spectrometry.
27325464	6	65	theme	Graphical	1260:1268	arg1	approaches					1306:1315	Graphical abstract Most hyphenated analytical approaches	1260:1315	Graphical abstract Most hyphenated analytical approaches that rely on liquid chromatography-MS	1260:1353	Graphical abstract Most hyphenated analytical approaches that rely on liquid chromatography-MS require relatively long separation times, produce incomplete resolution of oligosaccharide mixtures, use eluents that are incompatible with electrospray ionization, or require oligosaccharide derivatization.
27325464	4	66	theme	disaccharide	922:933	arg1	analysis					949:956	disaccharide compositional analysis	922:956	disaccharide compositional analysis	922:956	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	1	67	theme	mixtures	289:296	arg1	resolution					259:268	incomplete resolution	248:268	incomplete resolution of oligosaccharide mixtures	248:296	Most hyphenated analytical approaches that rely on liquid chromatography-MS require relatively long separation times, produce incomplete resolution of oligosaccharide mixtures, use eluents that are incompatible with electrospray ionization, or require oligosaccharide derivatization.
27325464	7	68	theme	molecular	1695:1703	arg1	heparin					1712:1718	a low molecular weight heparin	1689:1718	a low molecular weight heparin	1689:1718	Here we demonstrate the analysis of heparin oligosaccharides, including disaccharides, ultralow molecular weight heparin, and a low molecular weight heparin, using a novel electrokinetic pump-based CE-MS coupling eletrospray ion source.
27325464	5	69	theme	low	1144:1146	arg1	level					1148:1152	the low level	1140:1152	the low level of sulfate group loss that is achieved	1140:1191	The application of this CE-MS method to ultralow molecular heparin suggests that a charge state distribution and the low level of sulfate group loss that is achieved make this method useful for online tandem MS analysis of heparins.
27325464	10	70	theme	group	2322:2326	arg1	loss					2328:2331	sulfate group loss	2314:2331	sulfate group loss that is achieved	2314:2348	The application of this CE-MS method to ultralow molecular heparin suggests that a charge state distribution and the low level of sulfate group loss that is achieved make this method useful for online tandem MS analysis of heparins.
27325464	8	71	theme	polarity	1808:1815	arg1	separation					1820:1829	Reverse polarity CE separation	1800:1829	Reverse polarity CE separation	1800:1829	Reverse polarity CE separation and negative-mode electrospray ionization were optimized using a volatile methanolic ammonium acetate electrolyte and sheath fluid.
27325464	3	72	theme	sheath	792:797	arg1	fluid					799:803	sheath fluid	792:803	sheath fluid	792:803	Reverse polarity CE separation and negative-mode electrospray ionization were optimized using a volatile methanolic ammonium acetate electrolyte and sheath fluid.
27325464	7	73	theme	CE-MS	1761:1765	arg1	source					1792:1797	a novel electrokinetic pump-based CE-MS coupling eletrospray ion source	1727:1797	a novel electrokinetic pump-based CE-MS coupling eletrospray ion source	1727:1797	Here we demonstrate the analysis of heparin oligosaccharides, including disaccharides, ultralow molecular weight heparin, and a low molecular weight heparin, using a novel electrokinetic pump-based CE-MS coupling eletrospray ion source.
27325464	2	74	theme	oligosaccharides	450:465	arg1	analysis					430:437	the analysis	426:437	the analysis of heparin oligosaccharides, including disaccharides, ultralow molecular weight heparin, and a low molecular weight heparin	426:561	Here we demonstrate the analysis of heparin oligosaccharides, including disaccharides, ultralow molecular weight heparin, and a low molecular weight heparin, using a novel electrokinetic pump-based CE-MS coupling eletrospray ion source.
27325464	3	75	dep	acetate	768:774	arg1	electrolyte					776:786	electrolyte	776:786	electrolyte	776:786	Reverse polarity CE separation and negative-mode electrospray ionization were optimized using a volatile methanolic ammonium acetate electrolyte and sheath fluid.
27325464	3	75	dep	acetate	768:774	arg1	fluid					799:803	sheath fluid	792:803	sheath fluid	792:803	Reverse polarity CE separation and negative-mode electrospray ionization were optimized using a volatile methanolic ammonium acetate electrolyte and sheath fluid.
27325464	9	76	theme	compositional	2092:2104	arg1	analysis					2106:2113	disaccharide compositional analysis	2079:2113	disaccharide compositional analysis	2079:2113	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	8	77	theme	methanolic	1905:1914	arg1	acetate					1925:1931	a volatile methanolic ammonium acetate electrolyte and sheath fluid	1894:1960	a volatile methanolic ammonium acetate electrolyte and sheath fluid	1894:1960	Reverse polarity CE separation and negative-mode electrospray ionization were optimized using a volatile methanolic ammonium acetate electrolyte and sheath fluid.
27325464	4	78	theme	weight	1011:1016	arg1	heparin					1018:1024	low molecular weight heparin	997:1024	low molecular weight heparin	997:1024	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	9	79	theme	low	2154:2156	arg1	heparin					2175:2181	low molecular weight heparin	2154:2181	low molecular weight heparin	2154:2181	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	8	80	theme	negative-mode	1835:1847	arg1	ionization					1862:1871	negative-mode electrospray ionization	1835:1871	negative-mode electrospray ionization	1835:1871	Reverse polarity CE separation and negative-mode electrospray ionization were optimized using a volatile methanolic ammonium acetate electrolyte and sheath fluid.
27325464	7	81	theme	weight	1669:1674	arg1	heparin					1676:1682	ultralow molecular weight heparin	1650:1682	ultralow molecular weight heparin	1650:1682	Here we demonstrate the analysis of heparin oligosaccharides, including disaccharides, ultralow molecular weight heparin, and a low molecular weight heparin, using a novel electrokinetic pump-based CE-MS coupling eletrospray ion source.
27325464	9	82	theme	weight	2168:2173	arg1	heparin					2175:2181	low molecular weight heparin	2154:2181	low molecular weight heparin	2154:2181	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	6	83	theme	oligosaccharide	1430:1444	arg1	mixtures					1446:1453	oligosaccharide mixtures	1430:1453	oligosaccharide mixtures	1430:1453	Graphical abstract Most hyphenated analytical approaches that rely on liquid chromatography-MS require relatively long separation times, produce incomplete resolution of oligosaccharide mixtures, use eluents that are incompatible with electrospray ionization, or require oligosaccharide derivatization.
27325464	1	84	theme	separation	222:231	arg1	times					233:237	relatively long separation times	206:237	relatively long separation times	206:237	Most hyphenated analytical approaches that rely on liquid chromatography-MS require relatively long separation times, produce incomplete resolution of oligosaccharide mixtures, use eluents that are incompatible with electrospray ionization, or require oligosaccharide derivatization.
27325464	7	85	theme	ultralow	1650:1657	arg1	heparin					1676:1682	ultralow molecular weight heparin	1650:1682	ultralow molecular weight heparin	1650:1682	Here we demonstrate the analysis of heparin oligosaccharides, including disaccharides, ultralow molecular weight heparin, and a low molecular weight heparin, using a novel electrokinetic pump-based CE-MS coupling eletrospray ion source.
27325464	4	86	theme	low	997:999	arg1	heparin					1018:1024	low molecular weight heparin	997:1024	low molecular weight heparin	997:1024	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	6	87	theme	analytical	1295:1304	arg1	approaches					1306:1315	Graphical abstract Most hyphenated analytical approaches	1260:1315	Graphical abstract Most hyphenated analytical approaches that rely on liquid chromatography-MS	1260:1353	Graphical abstract Most hyphenated analytical approaches that rely on liquid chromatography-MS require relatively long separation times, produce incomplete resolution of oligosaccharide mixtures, use eluents that are incompatible with electrospray ionization, or require oligosaccharide derivatization.
27325464	10	88	theme	ultralow	2224:2231	arg1	heparin					2243:2249	ultralow molecular heparin	2224:2249	ultralow molecular heparin	2224:2249	The application of this CE-MS method to ultralow molecular heparin suggests that a charge state distribution and the low level of sulfate group loss that is achieved make this method useful for online tandem MS analysis of heparins.
27325464	3	89	theme	polarity	651:658	arg1	separation					663:672	Reverse polarity CE separation	643:672	Reverse polarity CE separation	643:672	Reverse polarity CE separation and negative-mode electrospray ionization were optimized using a volatile methanolic ammonium acetate electrolyte and sheath fluid.
27325464	1	90	theme	incomplete	248:257	arg1	resolution					259:268	incomplete resolution	248:268	incomplete resolution of oligosaccharide mixtures	248:296	Most hyphenated analytical approaches that rely on liquid chromatography-MS require relatively long separation times, produce incomplete resolution of oligosaccharide mixtures, use eluents that are incompatible with electrospray ionization, or require oligosaccharide derivatization.
27325464	7	91	theme	oligosaccharides	1607:1622	arg1	analysis					1587:1594	the analysis	1583:1594	the analysis of heparin oligosaccharides, including disaccharides, ultralow molecular weight heparin, and a low molecular weight heparin	1583:1718	Here we demonstrate the analysis of heparin oligosaccharides, including disaccharides, ultralow molecular weight heparin, and a low molecular weight heparin, using a novel electrokinetic pump-based CE-MS coupling eletrospray ion source.
27325464	5	92	theme	CE-MS	1051:1055	arg1	method					1057:1062	this CE-MS method	1046:1062	this CE-MS method	1046:1062	The application of this CE-MS method to ultralow molecular heparin suggests that a charge state distribution and the low level of sulfate group loss that is achieved make this method useful for online tandem MS analysis of heparins.
27325464	10	93	theme	MS	2392:2393	arg1	analysis					2395:2402	online tandem MS analysis	2378:2402	online tandem MS analysis of heparins	2378:2414	The application of this CE-MS method to ultralow molecular heparin suggests that a charge state distribution and the low level of sulfate group loss that is achieved make this method useful for online tandem MS analysis of heparins.
27325464	10	94	theme	method	2214:2219	arg1	application					2188:2198	The application	2184:2198	The application of this CE-MS method to ultralow molecular heparin	2184:2249	The application of this CE-MS method to ultralow molecular heparin suggests that a charge state distribution and the low level of sulfate group loss that is achieved make this method useful for online tandem MS analysis of heparins.
27325464	6	95	with	incompatible	1477:1488	arg1	ionization					1508:1517	electrospray ionization	1495:1517	electrospray ionization	1495:1517	Graphical abstract Most hyphenated analytical approaches that rely on liquid chromatography-MS require relatively long separation times, produce incomplete resolution of oligosaccharide mixtures, use eluents that are incompatible with electrospray ionization, or require oligosaccharide derivatization.
27325464	2	96	theme	ion	631:633	arg1	source					635:640	a novel electrokinetic pump-based CE-MS coupling eletrospray ion source	570:640	a novel electrokinetic pump-based CE-MS coupling eletrospray ion source	570:640	Here we demonstrate the analysis of heparin oligosaccharides, including disaccharides, ultralow molecular weight heparin, and a low molecular weight heparin, using a novel electrokinetic pump-based CE-MS coupling eletrospray ion source.
27325464	5	97	theme	online	1221:1226	arg1	analysis					1238:1245	online tandem MS analysis	1221:1245	online tandem MS analysis of heparins	1221:1257	The application of this CE-MS method to ultralow molecular heparin suggests that a charge state distribution and the low level of sulfate group loss that is achieved make this method useful for online tandem MS analysis of heparins.
27325464	3	98	theme	negative-mode	678:690	arg1	ionization					705:714	negative-mode electrospray ionization	678:714	negative-mode electrospray ionization	678:714	Reverse polarity CE separation and negative-mode electrospray ionization were optimized using a volatile methanolic ammonium acetate electrolyte and sheath fluid.
27325464	5	99	theme	method	1057:1062	arg1	application					1031:1041	The application	1027:1041	The application of this CE-MS method to ultralow molecular heparin	1027:1092	The application of this CE-MS method to ultralow molecular heparin suggests that a charge state distribution and the low level of sulfate group loss that is achieved make this method useful for online tandem MS analysis of heparins.
27325464	9	100	theme	online	1967:1972	arg1	MS					2025:2026	The online CE hyphenated negative-ion electrospray ionization MS	1963:2026	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer	1963:2063	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	9	100	theme	online	1967:1972	arg1	useful					2069:2074	useful	2069:2074	useful	2069:2074	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	6	101	theme	separation	1379:1388	arg1	times					1390:1394	relatively long separation times	1363:1394	relatively long separation times	1363:1394	Graphical abstract Most hyphenated analytical approaches that rely on liquid chromatography-MS require relatively long separation times, produce incomplete resolution of oligosaccharide mixtures, use eluents that are incompatible with electrospray ionization, or require oligosaccharide derivatization.
27325464	4	102	theme	negative-ion	831:842	arg1	useful					912:917	useful	912:917	useful	912:917	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	4	102	theme	negative-ion	831:842	arg1	MS					868:869	The online CE hyphenated negative-ion electrospray ionization MS	806:869	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer	806:906	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	5	103	theme	ultralow	1067:1074	arg1	heparin					1086:1092	ultralow molecular heparin	1067:1092	ultralow molecular heparin	1067:1092	The application of this CE-MS method to ultralow molecular heparin suggests that a charge state distribution and the low level of sulfate group loss that is achieved make this method useful for online tandem MS analysis of heparins.
27325464	9	104	theme	hyphenated	1977:1986	arg1	MS					2025:2026	The online CE hyphenated negative-ion electrospray ionization MS	1963:2026	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer	1963:2063	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	9	104	theme	hyphenated	1977:1986	arg1	useful					2069:2074	useful	2069:2074	useful	2069:2074	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	4	105	theme	ionization	857:866	arg1	useful					912:917	useful	912:917	useful	912:917	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	4	105	theme	ionization	857:866	arg1	MS					868:869	The online CE hyphenated negative-ion electrospray ionization MS	806:869	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer	806:906	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	0	106	theme	mass	103:106	arg1	spectrometry					108:119	capillary electrophoresis-negative-ion electrospray ionization mass spectrometry	40:119	capillary electrophoresis-negative-ion electrospray ionization mass spectrometry	40:119	Analysis of heparin oligosaccharides by capillary electrophoresis-negative-ion electrospray ionization mass spectrometry.
27325464	10	107	theme	loss	2328:2331	arg1	distribution					2280:2291	a charge state distribution	2265:2291	a charge state distribution	2265:2291	The application of this CE-MS method to ultralow molecular heparin suggests that a charge state distribution and the low level of sulfate group loss that is achieved make this method useful for online tandem MS analysis of heparins.
27325464	10	107	theme	loss	2328:2331	arg1	level					2305:2309	the low level	2297:2309	the low level of sulfate group loss that is achieved	2297:2348	The application of this CE-MS method to ultralow molecular heparin suggests that a charge state distribution and the low level of sulfate group loss that is achieved make this method useful for online tandem MS analysis of heparins.
27325464	7	108	theme	ion	1788:1790	arg1	source					1792:1797	a novel electrokinetic pump-based CE-MS coupling eletrospray ion source	1727:1797	a novel electrokinetic pump-based CE-MS coupling eletrospray ion source	1727:1797	Here we demonstrate the analysis of heparin oligosaccharides, including disaccharides, ultralow molecular weight heparin, and a low molecular weight heparin, using a novel electrokinetic pump-based CE-MS coupling eletrospray ion source.
27325464	2	109	theme	novel	572:576	arg1	source					635:640	a novel electrokinetic pump-based CE-MS coupling eletrospray ion source	570:640	a novel electrokinetic pump-based CE-MS coupling eletrospray ion source	570:640	Here we demonstrate the analysis of heparin oligosaccharides, including disaccharides, ultralow molecular weight heparin, and a low molecular weight heparin, using a novel electrokinetic pump-based CE-MS coupling eletrospray ion source.
27325464	10	110	theme	charge	2267:2272	arg1	distribution					2280:2291	a charge state distribution	2265:2291	a charge state distribution	2265:2291	The application of this CE-MS method to ultralow molecular heparin suggests that a charge state distribution and the low level of sulfate group loss that is achieved make this method useful for online tandem MS analysis of heparins.
27325464	7	111	theme	coupling	1767:1774	arg1	source					1792:1797	a novel electrokinetic pump-based CE-MS coupling eletrospray ion source	1727:1797	a novel electrokinetic pump-based CE-MS coupling eletrospray ion source	1727:1797	Here we demonstrate the analysis of heparin oligosaccharides, including disaccharides, ultralow molecular weight heparin, and a low molecular weight heparin, using a novel electrokinetic pump-based CE-MS coupling eletrospray ion source.
27325464	9	112	theme	mass	2047:2050	arg1	spectrometer					2052:2063	an LTQ Orbitrap mass spectrometer	2031:2063	an LTQ Orbitrap mass spectrometer	2031:2063	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	4	113	theme	CE	817:818	arg1	useful					912:917	useful	912:917	useful	912:917	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	4	113	theme	CE	817:818	arg1	MS					868:869	The online CE hyphenated negative-ion electrospray ionization MS	806:869	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer	806:906	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	5	114	theme	charge	1110:1115	arg1	distribution					1123:1134	a charge state distribution	1108:1134	a charge state distribution	1108:1134	The application of this CE-MS method to ultralow molecular heparin suggests that a charge state distribution and the low level of sulfate group loss that is achieved make this method useful for online tandem MS analysis of heparins.
27325464	6	115	theme	Most	1279:1282	arg1	approaches					1306:1315	Graphical abstract Most hyphenated analytical approaches	1260:1315	Graphical abstract Most hyphenated analytical approaches that rely on liquid chromatography-MS	1260:1353	Graphical abstract Most hyphenated analytical approaches that rely on liquid chromatography-MS require relatively long separation times, produce incomplete resolution of oligosaccharide mixtures, use eluents that are incompatible with electrospray ionization, or require oligosaccharide derivatization.
27325464	7	116	theme	pump-based	1750:1759	arg1	source					1792:1797	a novel electrokinetic pump-based CE-MS coupling eletrospray ion source	1727:1797	a novel electrokinetic pump-based CE-MS coupling eletrospray ion source	1727:1797	Here we demonstrate the analysis of heparin oligosaccharides, including disaccharides, ultralow molecular weight heparin, and a low molecular weight heparin, using a novel electrokinetic pump-based CE-MS coupling eletrospray ion source.
27325464	2	117	theme	coupling	610:617	arg1	source					635:640	a novel electrokinetic pump-based CE-MS coupling eletrospray ion source	570:640	a novel electrokinetic pump-based CE-MS coupling eletrospray ion source	570:640	Here we demonstrate the analysis of heparin oligosaccharides, including disaccharides, ultralow molecular weight heparin, and a low molecular weight heparin, using a novel electrokinetic pump-based CE-MS coupling eletrospray ion source.
27325464	4	118	theme	mass	890:893	arg1	spectrometer					895:906	an LTQ Orbitrap mass spectrometer	874:906	an LTQ Orbitrap mass spectrometer	874:906	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	6	119	theme	oligosaccharide	1531:1545	arg1	derivatization					1547:1560	oligosaccharide derivatization	1531:1560	oligosaccharide derivatization	1531:1560	Graphical abstract Most hyphenated analytical approaches that rely on liquid chromatography-MS require relatively long separation times, produce incomplete resolution of oligosaccharide mixtures, use eluents that are incompatible with electrospray ionization, or require oligosaccharide derivatization.
27325464	5	120	theme	MS	1235:1236	arg1	analysis					1238:1245	online tandem MS analysis	1221:1245	online tandem MS analysis of heparins	1221:1257	The application of this CE-MS method to ultralow molecular heparin suggests that a charge state distribution and the low level of sulfate group loss that is achieved make this method useful for online tandem MS analysis of heparins.
27325464	2	121	theme	pump-based	593:602	arg1	source					635:640	a novel electrokinetic pump-based CE-MS coupling eletrospray ion source	570:640	a novel electrokinetic pump-based CE-MS coupling eletrospray ion source	570:640	Here we demonstrate the analysis of heparin oligosaccharides, including disaccharides, ultralow molecular weight heparin, and a low molecular weight heparin, using a novel electrokinetic pump-based CE-MS coupling eletrospray ion source.
27325464	9	122	theme	LTQ	2034:2036	arg1	spectrometer					2052:2063	an LTQ Orbitrap mass spectrometer	2031:2063	an LTQ Orbitrap mass spectrometer	2031:2063	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	4	123	from	analysis	985:992	arg1	MS					868:869	The online CE hyphenated negative-ion electrospray ionization MS	806:869	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer	806:906	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	4	123	from	analysis	985:992	arg1	useful					912:917	useful	912:917	useful	912:917	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	1	124	theme	Most	122:125	arg1	approaches					149:158	Most hyphenated analytical approaches	122:158	Most hyphenated analytical approaches that rely on liquid chromatography-MS	122:196	Most hyphenated analytical approaches that rely on liquid chromatography-MS require relatively long separation times, produce incomplete resolution of oligosaccharide mixtures, use eluents that are incompatible with electrospray ionization, or require oligosaccharide derivatization.
27325464	1	125	theme	analytical	138:147	arg1	approaches					149:158	Most hyphenated analytical approaches	122:158	Most hyphenated analytical approaches that rely on liquid chromatography-MS	122:196	Most hyphenated analytical approaches that rely on liquid chromatography-MS require relatively long separation times, produce incomplete resolution of oligosaccharide mixtures, use eluents that are incompatible with electrospray ionization, or require oligosaccharide derivatization.
27325464	4	126	theme	LTQ	877:879	arg1	spectrometer					895:906	an LTQ Orbitrap mass spectrometer	874:906	an LTQ Orbitrap mass spectrometer	874:906	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	2	127	theme	weight	512:517	arg1	heparin					519:525	ultralow molecular weight heparin	493:525	ultralow molecular weight heparin	493:525	Here we demonstrate the analysis of heparin oligosaccharides, including disaccharides, ultralow molecular weight heparin, and a low molecular weight heparin, using a novel electrokinetic pump-based CE-MS coupling eletrospray ion source.
27325464	0	128	theme	capillary	40:48	arg1	spectrometry					108:119	capillary electrophoresis-negative-ion electrospray ionization mass spectrometry	40:119	capillary electrophoresis-negative-ion electrospray ionization mass spectrometry	40:119	Analysis of heparin oligosaccharides by capillary electrophoresis-negative-ion electrospray ionization mass spectrometry.
27325464	7	129	theme	electrokinetic	1735:1748	arg1	source					1792:1797	a novel electrokinetic pump-based CE-MS coupling eletrospray ion source	1727:1797	a novel electrokinetic pump-based CE-MS coupling eletrospray ion source	1727:1797	Here we demonstrate the analysis of heparin oligosaccharides, including disaccharides, ultralow molecular weight heparin, and a low molecular weight heparin, using a novel electrokinetic pump-based CE-MS coupling eletrospray ion source.
27325464	5	130	theme	sulfate	1157:1163	arg1	loss					1171:1174	sulfate group loss	1157:1174	sulfate group loss that is achieved	1157:1191	The application of this CE-MS method to ultralow molecular heparin suggests that a charge state distribution and the low level of sulfate group loss that is achieved make this method useful for online tandem MS analysis of heparins.
27325464	10	131	theme	sulfate	2314:2320	arg1	loss					2328:2331	sulfate group loss	2314:2331	sulfate group loss that is achieved	2314:2348	The application of this CE-MS method to ultralow molecular heparin suggests that a charge state distribution and the low level of sulfate group loss that is achieved make this method useful for online tandem MS analysis of heparins.
27325464	9	132	theme	disaccharide	2079:2090	arg1	analysis					2106:2113	disaccharide compositional analysis	2079:2113	disaccharide compositional analysis	2079:2113	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	3	133	theme	methanolic	748:757	arg1	acetate					768:774	a volatile methanolic ammonium acetate electrolyte and sheath fluid	737:803	a volatile methanolic ammonium acetate electrolyte and sheath fluid	737:803	Reverse polarity CE separation and negative-mode electrospray ionization were optimized using a volatile methanolic ammonium acetate electrolyte and sheath fluid.
27325464	2	134	theme	ultralow	493:500	arg1	heparin					519:525	ultralow molecular weight heparin	493:525	ultralow molecular weight heparin	493:525	Here we demonstrate the analysis of heparin oligosaccharides, including disaccharides, ultralow molecular weight heparin, and a low molecular weight heparin, using a novel electrokinetic pump-based CE-MS coupling eletrospray ion source.
27325464	0	135	theme	electrospray	79:90	arg1	spectrometry					108:119	capillary electrophoresis-negative-ion electrospray ionization mass spectrometry	40:119	capillary electrophoresis-negative-ion electrospray ionization mass spectrometry	40:119	Analysis of heparin oligosaccharides by capillary electrophoresis-negative-ion electrospray ionization mass spectrometry.
27325464	1	136	theme	oligosaccharide	374:388	arg1	derivatization					390:403	oligosaccharide derivatization	374:403	oligosaccharide derivatization	374:403	Most hyphenated analytical approaches that rely on liquid chromatography-MS require relatively long separation times, produce incomplete resolution of oligosaccharide mixtures, use eluents that are incompatible with electrospray ionization, or require oligosaccharide derivatization.
27325464	5	137	theme	loss	1171:1174	arg1	level					1148:1152	the low level	1140:1152	the low level of sulfate group loss that is achieved	1140:1191	The application of this CE-MS method to ultralow molecular heparin suggests that a charge state distribution and the low level of sulfate group loss that is achieved make this method useful for online tandem MS analysis of heparins.
27325464	5	137	theme	loss	1171:1174	arg1	distribution					1123:1134	a charge state distribution	1108:1134	a charge state distribution	1108:1134	The application of this CE-MS method to ultralow molecular heparin suggests that a charge state distribution and the low level of sulfate group loss that is achieved make this method useful for online tandem MS analysis of heparins.
27325464	6	138	theme	abstract	1270:1277	arg1	approaches					1306:1315	Graphical abstract Most hyphenated analytical approaches	1260:1315	Graphical abstract Most hyphenated analytical approaches that rely on liquid chromatography-MS	1260:1353	Graphical abstract Most hyphenated analytical approaches that rely on liquid chromatography-MS require relatively long separation times, produce incomplete resolution of oligosaccharide mixtures, use eluents that are incompatible with electrospray ionization, or require oligosaccharide derivatization.
27325464	2	139	theme	molecular	538:546	arg1	heparin					555:561	a low molecular weight heparin	532:561	a low molecular weight heparin	532:561	Here we demonstrate the analysis of heparin oligosaccharides, including disaccharides, ultralow molecular weight heparin, and a low molecular weight heparin, using a novel electrokinetic pump-based CE-MS coupling eletrospray ion source.
27325464	8	140	theme	Reverse	1800:1806	arg1	separation					1820:1829	Reverse polarity CE separation	1800:1829	Reverse polarity CE separation	1800:1829	Reverse polarity CE separation and negative-mode electrospray ionization were optimized using a volatile methanolic ammonium acetate electrolyte and sheath fluid.
27325464	9	141	theme	Orbitrap	2038:2045	arg1	spectrometer					2052:2063	an LTQ Orbitrap mass spectrometer	2031:2063	an LTQ Orbitrap mass spectrometer	2031:2063	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	4	142	from	MS	868:869	arg1	spectrometer					895:906	an LTQ Orbitrap mass spectrometer	874:906	an LTQ Orbitrap mass spectrometer	874:906	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	9	143	from	MS	2025:2026	arg1	spectrometer					2052:2063	an LTQ Orbitrap mass spectrometer	2031:2063	an LTQ Orbitrap mass spectrometer	2031:2063	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	9	144	theme	bottom-up	2119:2127	arg1	analysis					2142:2149	bottom-up and top-down analysis	2119:2149	analysis	2142:2149	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	8	145	theme	CE	1817:1818	arg1	separation					1820:1829	Reverse polarity CE separation	1800:1829	Reverse polarity CE separation	1800:1829	Reverse polarity CE separation and negative-mode electrospray ionization were optimized using a volatile methanolic ammonium acetate electrolyte and sheath fluid.
27325464	8	146	theme	volatile	1896:1903	arg1	acetate					1925:1931	a volatile methanolic ammonium acetate electrolyte and sheath fluid	1894:1960	a volatile methanolic ammonium acetate electrolyte and sheath fluid	1894:1960	Reverse polarity CE separation and negative-mode electrospray ionization were optimized using a volatile methanolic ammonium acetate electrolyte and sheath fluid.
27325464	7	147	theme	weight	1705:1710	arg1	heparin					1712:1718	a low molecular weight heparin	1689:1718	a low molecular weight heparin	1689:1718	Here we demonstrate the analysis of heparin oligosaccharides, including disaccharides, ultralow molecular weight heparin, and a low molecular weight heparin, using a novel electrokinetic pump-based CE-MS coupling eletrospray ion source.
27325464	9	148	theme	top-down	2133:2140	arg1	analysis					2142:2149	bottom-up and top-down analysis	2119:2149	analysis	2142:2149	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	4	149	theme	compositional	935:947	arg1	analysis					949:956	disaccharide compositional analysis	922:956	disaccharide compositional analysis	922:956	The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin.
27325464	2	150	theme	heparin	442:448	arg1	disaccharides					478:490	disaccharides	478:490	disaccharides	478:490	Here we demonstrate the analysis of heparin oligosaccharides, including disaccharides, ultralow molecular weight heparin, and a low molecular weight heparin, using a novel electrokinetic pump-based CE-MS coupling eletrospray ion source.
27325464	2	150	theme	heparin	442:448	arg1	heparin					519:525	ultralow molecular weight heparin	493:525	ultralow molecular weight heparin	493:525	Here we demonstrate the analysis of heparin oligosaccharides, including disaccharides, ultralow molecular weight heparin, and a low molecular weight heparin, using a novel electrokinetic pump-based CE-MS coupling eletrospray ion source.
27325464	2	150	theme	heparin	442:448	arg1	heparin					555:561	a low molecular weight heparin	532:561	a low molecular weight heparin	532:561	Here we demonstrate the analysis of heparin oligosaccharides, including disaccharides, ultralow molecular weight heparin, and a low molecular weight heparin, using a novel electrokinetic pump-based CE-MS coupling eletrospray ion source.
27325464	2	150	theme	heparin	442:448	arg1	oligosaccharides					450:465	heparin oligosaccharides	442:465	heparin oligosaccharides	442:465	Here we demonstrate the analysis of heparin oligosaccharides, including disaccharides, ultralow molecular weight heparin, and a low molecular weight heparin, using a novel electrokinetic pump-based CE-MS coupling eletrospray ion source.
27325464	8	151	theme	ammonium	1916:1923	arg1	acetate					1925:1931	a volatile methanolic ammonium acetate electrolyte and sheath fluid	1894:1960	a volatile methanolic ammonium acetate electrolyte and sheath fluid	1894:1960	Reverse polarity CE separation and negative-mode electrospray ionization were optimized using a volatile methanolic ammonium acetate electrolyte and sheath fluid.
25138067	0	0	theme	penetrated	89:98	arg1	papillae					100:107	nonpenetrated versus penetrated papillae	68:107	nonpenetrated versus penetrated papillae on leaves of barley	68:127	Differential accumulation of callose, arabinoxylan and cellulose in nonpenetrated versus penetrated papillae on leaves of barley infected with Blumeria graminis f. sp.
25138067	9	1	theme	layered	1232:1238	arg1	structure					1240:1248	a layered structure	1230:1248	a layered structure	1230:1248	The papillae have a layered structure, with the inner core consisting of callose and arabinoxylan and the outer layer containing arabinoxylan and cellulose.
25138067	7	2	theme	cellulose	1054:1062	arg1	concentrations					1036:1049	significant concentrations	1024:1049	significant concentrations of cellulose and arabinoxylan	1024:1079	The data show that barley papillae induced during infection with Bgh contain, in addition to callose, significant concentrations of cellulose and arabinoxylan.
25138067	3	3	theme	physical	446:453	arg1	forces					468:473	the physical and chemical forces	442:473	the physical and chemical forces applied by the fungus	442:495	For the plant cell to block penetration attempts, it must adapt its cell wall to withstand the physical and chemical forces applied by the fungus.
25138067	10	4	theme	composition	1495:1505	arg1	improvement					1472:1482	the improvement	1468:1482	the improvement of papilla composition and enhanced disease resistance	1468:1537	The association of arabinoxylan and cellulose with penetration resistance suggests new targets for the improvement of papilla composition and enhanced disease resistance.
25138067	5	5	theme	Hordeum	720:726	arg1	barley					712:717	barley	712:717	barley (Hordeum vulgare) attacked by the powdery mildew fungus Blumeria graminis f. sp	712:797	Here, we have re-examined the composition of papillae of barley (Hordeum vulgare) attacked by the powdery mildew fungus Blumeria graminis f. sp.
25138067	5	5	theme	Hordeum	720:726	arg1	vulgare					728:734	Hordeum vulgare	720:734	Hordeum vulgare	720:734	Here, we have re-examined the composition of papillae of barley (Hordeum vulgare) attacked by the powdery mildew fungus Blumeria graminis f. sp.
25138067	0	6	from	accumulation	13:24	arg1	leaves					112:117	leaves	112:117	leaves	112:117	Differential accumulation of callose, arabinoxylan and cellulose in nonpenetrated versus penetrated papillae on leaves of barley infected with Blumeria graminis f. sp.
25138067	0	6	from	accumulation	13:24	arg1	papillae					100:107	nonpenetrated versus penetrated papillae	68:107	nonpenetrated versus penetrated papillae on leaves of barley	68:127	Differential accumulation of callose, arabinoxylan and cellulose in nonpenetrated versus penetrated papillae on leaves of barley infected with Blumeria graminis f. sp.
25138067	7	7	theme	arabinoxylan	1068:1079	arg1	concentrations					1036:1049	significant concentrations	1024:1049	significant concentrations of cellulose and arabinoxylan	1024:1079	The data show that barley papillae induced during infection with Bgh contain, in addition to callose, significant concentrations of cellulose and arabinoxylan.
25138067	10	8	theme	cellulose	1405:1413	arg1	association					1373:1383	The association	1369:1383	The association of arabinoxylan and cellulose with penetration resistance	1369:1441	The association of arabinoxylan and cellulose with penetration resistance suggests new targets for the improvement of papilla composition and enhanced disease resistance.
25138067	8	9	theme	callose	1107:1113	arg1	concentrations					1089:1102	Higher concentrations	1082:1102	Higher concentrations of callose, arabinoxylan and cellulose	1082:1141	Higher concentrations of callose, arabinoxylan and cellulose are found in effective papillae, compared with ineffective papillae.
25138067	10	10	theme	resistance	1528:1537	arg1	improvement					1472:1482	the improvement	1468:1482	the improvement of papilla composition and enhanced disease resistance	1468:1537	The association of arabinoxylan and cellulose with penetration resistance suggests new targets for the improvement of papilla composition and enhanced disease resistance.
25138067	3	11	theme	chemical	459:466	arg1	forces					468:473	the physical and chemical forces	442:473	the physical and chemical forces applied by the fungus	442:495	For the plant cell to block penetration attempts, it must adapt its cell wall to withstand the physical and chemical forces applied by the fungus.
25138067	2	12	theme	successful	262:271	arg1	invasion					273:280	successful invasion	262:280	successful invasion by fungal pathogens	262:300	In plants, cell walls are one of the first lines of defence for protecting cells from successful invasion by fungal pathogens and are a major factor in basal host resistance.
25138067	9	13	contain	containing	1330:1339	arg1	layer					1324:1328	the outer layer	1314:1328	the outer layer containing arabinoxylan and cellulose	1314:1366	The papillae have a layered structure, with the inner core consisting of callose and arabinoxylan and the outer layer containing arabinoxylan and cellulose.
25138067	9	13	contain	containing	1330:1339	arg2	cellulose					1358:1366	cellulose	1358:1366	cellulose	1358:1366	The papillae have a layered structure, with the inner core consisting of callose and arabinoxylan and the outer layer containing arabinoxylan and cellulose.
25138067	9	13	contain	containing	1330:1339	arg2	arabinoxylan					1341:1352	arabinoxylan	1341:1352	arabinoxylan	1341:1352	The papillae have a layered structure, with the inner core consisting of callose and arabinoxylan and the outer layer containing arabinoxylan and cellulose.
25138067	8	14	theme	arabinoxylan	1116:1127	arg1	concentrations					1089:1102	Higher concentrations	1082:1102	Higher concentrations of callose, arabinoxylan and cellulose	1082:1141	Higher concentrations of callose, arabinoxylan and cellulose are found in effective papillae, compared with ineffective papillae.
25138067	2	15	from	one	202:204	arg1	plants					179:184	plants	179:184	plants	179:184	In plants, cell walls are one of the first lines of defence for protecting cells from successful invasion by fungal pathogens and are a major factor in basal host resistance.
25138067	5	16	theme	papillae	700:707	arg1	composition					685:695	the composition	681:695	the composition of papillae of barley (Hordeum vulgare) attacked by the powdery mildew fungus Blumeria graminis f. sp	681:797	Here, we have re-examined the composition of papillae of barley (Hordeum vulgare) attacked by the powdery mildew fungus Blumeria graminis f. sp.
25138067	9	17	theme	inner	1260:1264	arg1	core					1266:1269	the inner core	1256:1269	the inner core consisting of callose and arabinoxylan	1256:1308	The papillae have a layered structure, with the inner core consisting of callose and arabinoxylan and the outer layer containing arabinoxylan and cellulose.
25138067	7	18	theme	significant	1024:1034	arg1	concentrations					1036:1049	significant concentrations	1024:1049	significant concentrations of cellulose and arabinoxylan	1024:1079	The data show that barley papillae induced during infection with Bgh contain, in addition to callose, significant concentrations of cellulose and arabinoxylan.
25138067	5	19	theme	powdery	753:759	arg1	graminis					784:791	the powdery mildew fungus Blumeria graminis	749:791	the powdery mildew fungus Blumeria graminis f. sp	749:797	Here, we have re-examined the composition of papillae of barley (Hordeum vulgare) attacked by the powdery mildew fungus Blumeria graminis f. sp.
25138067	7	20	with	infection	972:980	arg1	Bgh					987:989	Bgh	987:989	Bgh	987:989	The data show that barley papillae induced during infection with Bgh contain, in addition to callose, significant concentrations of cellulose and arabinoxylan.
25138067	8	21	theme	cellulose	1133:1141	arg1	concentrations					1089:1102	Higher concentrations	1082:1102	Higher concentrations of callose, arabinoxylan and cellulose	1082:1141	Higher concentrations of callose, arabinoxylan and cellulose are found in effective papillae, compared with ineffective papillae.
25138067	8	22	theme	Higher	1082:1087	arg1	concentrations					1089:1102	Higher concentrations	1082:1102	Higher concentrations of callose, arabinoxylan and cellulose	1082:1141	Higher concentrations of callose, arabinoxylan and cellulose are found in effective papillae, compared with ineffective papillae.
25138067	10	23	theme	penetration	1420:1430	arg1	resistance					1432:1441	penetration resistance	1420:1441	penetration resistance	1420:1441	The association of arabinoxylan and cellulose with penetration resistance suggests new targets for the improvement of papilla composition and enhanced disease resistance.
25138067	2	24	from	factor	318:323	arg1	resistance					339:348	basal host resistance	328:348	basal host resistance	328:348	In plants, cell walls are one of the first lines of defence for protecting cells from successful invasion by fungal pathogens and are a major factor in basal host resistance.
25138067	10	25	theme	papilla	1487:1493	arg1	composition					1495:1505	papilla composition	1487:1505	papilla composition	1487:1505	The association of arabinoxylan and cellulose with penetration resistance suggests new targets for the improvement of papilla composition and enhanced disease resistance.
25138067	4	26	theme	main	624:627	arg1	component					644:652	the main polysaccharide component	620:652	the main polysaccharide component	620:652	Papillae that have been effective in preventing penetration by pathogens are traditionally believed to contain callose as the main polysaccharide component.
25138067	4	26	theme	main	624:627	arg1	callose					609:615	callose	609:615	callose	609:615	Papillae that have been effective in preventing penetration by pathogens are traditionally believed to contain callose as the main polysaccharide component.
25138067	0	27	theme	Differential	0:11	arg1	accumulation					13:24	Differential accumulation	0:24	Differential accumulation of callose, arabinoxylan and cellulose in nonpenetrated versus penetrated papillae on leaves of barley	0:127	Differential accumulation of callose, arabinoxylan and cellulose in nonpenetrated versus penetrated papillae on leaves of barley infected with Blumeria graminis f. sp.
25138067	2	28	theme	fungal	285:290	arg1	pathogens					292:300	fungal pathogens	285:300	fungal pathogens	285:300	In plants, cell walls are one of the first lines of defence for protecting cells from successful invasion by fungal pathogens and are a major factor in basal host resistance.
25138067	8	29	theme	ineffective	1190:1200	arg1	papillae					1202:1209	ineffective papillae	1190:1209	ineffective papillae	1190:1209	Higher concentrations of callose, arabinoxylan and cellulose are found in effective papillae, compared with ineffective papillae.
25138067	0	30	theme	callose	29:35	arg1	accumulation					13:24	Differential accumulation	0:24	Differential accumulation of callose, arabinoxylan and cellulose in nonpenetrated versus penetrated papillae on leaves of barley	0:127	Differential accumulation of callose, arabinoxylan and cellulose in nonpenetrated versus penetrated papillae on leaves of barley infected with Blumeria graminis f. sp.
25138067	6	31	theme	wall	900:903	arg1	polysaccharides					905:919	cell wall polysaccharides	895:919	cell wall polysaccharides	895:919	hordei (Bgh) using a range of antibodies and carbohydrate-binding modules that are targeted to cell wall polysaccharides.
25138067	7	32	contain	contain	991:997	arg2	papillae					948:955	barley papillae	941:955	barley papillae induced during infection with Bgh	941:989	The data show that barley papillae induced during infection with Bgh contain, in addition to callose, significant concentrations of cellulose and arabinoxylan.
25138067	7	32	contain	contain	991:997	arg1	papillae					948:955	barley papillae	941:955	barley papillae induced during infection with Bgh	941:989	The data show that barley papillae induced during infection with Bgh contain, in addition to callose, significant concentrations of cellulose and arabinoxylan.
25138067	7	32	contain	contain	991:997	arg2	concentrations					1036:1049	significant concentrations	1024:1049	significant concentrations of cellulose and arabinoxylan	1024:1079	The data show that barley papillae induced during infection with Bgh contain, in addition to callose, significant concentrations of cellulose and arabinoxylan.
25138067	7	32	contain	contain	991:997	arg1	callose					1015:1021	callose	1015:1021	callose	1015:1021	The data show that barley papillae induced during infection with Bgh contain, in addition to callose, significant concentrations of cellulose and arabinoxylan.
25138067	8	33	theme	effective	1156:1164	arg1	papillae					1166:1173	effective papillae	1156:1173	effective papillae	1156:1173	Higher concentrations of callose, arabinoxylan and cellulose are found in effective papillae, compared with ineffective papillae.
25138067	3	34	dep	penetration	379:389	arg1	attempts					391:398	attempts	391:398	attempts	391:398	For the plant cell to block penetration attempts, it must adapt its cell wall to withstand the physical and chemical forces applied by the fungus.
25138067	5	35	theme	Blumeria	775:782	arg1	graminis					784:791	the powdery mildew fungus Blumeria graminis	749:791	the powdery mildew fungus Blumeria graminis f. sp	749:797	Here, we have re-examined the composition of papillae of barley (Hordeum vulgare) attacked by the powdery mildew fungus Blumeria graminis f. sp.
25138067	5	36	theme	fungus	768:773	arg1	graminis					784:791	the powdery mildew fungus Blumeria graminis	749:791	the powdery mildew fungus Blumeria graminis f. sp	749:797	Here, we have re-examined the composition of papillae of barley (Hordeum vulgare) attacked by the powdery mildew fungus Blumeria graminis f. sp.
25138067	6	37	theme	cell	895:898	arg1	polysaccharides					905:919	cell wall polysaccharides	895:919	cell wall polysaccharides	895:919	hordei (Bgh) using a range of antibodies and carbohydrate-binding modules that are targeted to cell wall polysaccharides.
25138067	10	38	theme	disease	1520:1526	arg1	resistance					1528:1537	enhanced disease resistance	1511:1537	enhanced disease resistance	1511:1537	The association of arabinoxylan and cellulose with penetration resistance suggests new targets for the improvement of papilla composition and enhanced disease resistance.
25138067	10	39	theme	enhanced	1511:1518	arg1	resistance					1528:1537	enhanced disease resistance	1511:1537	enhanced disease resistance	1511:1537	The association of arabinoxylan and cellulose with penetration resistance suggests new targets for the improvement of papilla composition and enhanced disease resistance.
25138067	3	40	theme	cell	419:422	arg1	wall					424:427	its cell wall	415:427	its cell wall	415:427	For the plant cell to block penetration attempts, it must adapt its cell wall to withstand the physical and chemical forces applied by the fungus.
25138067	0	41	theme	arabinoxylan	38:49	arg1	accumulation					13:24	Differential accumulation	0:24	Differential accumulation of callose, arabinoxylan and cellulose in nonpenetrated versus penetrated papillae on leaves of barley	0:127	Differential accumulation of callose, arabinoxylan and cellulose in nonpenetrated versus penetrated papillae on leaves of barley infected with Blumeria graminis f. sp.
25138067	0	42	theme	Blumeria	143:150	arg1	sp					164:165	Blumeria graminis f. sp	143:165	Blumeria graminis f. sp	143:165	Differential accumulation of callose, arabinoxylan and cellulose in nonpenetrated versus penetrated papillae on leaves of barley infected with Blumeria graminis f. sp.
25138067	4	43	contain	contain	601:607	arg1	Papillae					498:505	Papillae	498:505	Papillae that have been effective in preventing penetration by pathogens	498:569	Papillae that have been effective in preventing penetration by pathogens are traditionally believed to contain callose as the main polysaccharide component.
25138067	4	43	contain	contain	601:607	arg2	callose					609:615	callose	609:615	callose	609:615	Papillae that have been effective in preventing penetration by pathogens are traditionally believed to contain callose as the main polysaccharide component.
25138067	4	43	contain	contain	601:607	arg2	component					644:652	the main polysaccharide component	620:652	the main polysaccharide component	620:652	Papillae that have been effective in preventing penetration by pathogens are traditionally believed to contain callose as the main polysaccharide component.
25138067	2	44	theme	first	213:217	arg1	lines					219:223	the first lines	209:223	the first lines of defence for protecting cells from successful invasion by fungal pathogens	209:300	In plants, cell walls are one of the first lines of defence for protecting cells from successful invasion by fungal pathogens and are a major factor in basal host resistance.
25138067	5	45	theme	f.	793:794	arg1	sp					796:797	f. sp	793:797	the powdery mildew fungus Blumeria graminis f. sp	749:797	Here, we have re-examined the composition of papillae of barley (Hordeum vulgare) attacked by the powdery mildew fungus Blumeria graminis f. sp.
25138067	7	46	dep	callose	1015:1021	arg1	addition					1003:1010	addition	1003:1010	addition	1003:1010	The data show that barley papillae induced during infection with Bgh contain, in addition to callose, significant concentrations of cellulose and arabinoxylan.
25138067	9	47	theme	outer	1318:1322	arg1	layer					1324:1328	the outer layer	1314:1328	the outer layer containing arabinoxylan and cellulose	1314:1366	The papillae have a layered structure, with the inner core consisting of callose and arabinoxylan and the outer layer containing arabinoxylan and cellulose.
25138067	5	48	theme	mildew	761:766	arg1	graminis					784:791	the powdery mildew fungus Blumeria graminis	749:791	the powdery mildew fungus Blumeria graminis f. sp	749:797	Here, we have re-examined the composition of papillae of barley (Hordeum vulgare) attacked by the powdery mildew fungus Blumeria graminis f. sp.
25138067	6	49	theme	modules	866:872	arg1	range					821:825	a range	819:825	a range of antibodies and carbohydrate-binding modules that are targeted to cell wall polysaccharides	819:919	hordei (Bgh) using a range of antibodies and carbohydrate-binding modules that are targeted to cell wall polysaccharides.
25138067	0	50	theme	cellulose	55:63	arg1	accumulation					13:24	Differential accumulation	0:24	Differential accumulation of callose, arabinoxylan and cellulose in nonpenetrated versus penetrated papillae on leaves of barley	0:127	Differential accumulation of callose, arabinoxylan and cellulose in nonpenetrated versus penetrated papillae on leaves of barley infected with Blumeria graminis f. sp.
25138067	0	51	theme	f.	161:162	arg1	sp					164:165	Blumeria graminis f. sp	143:165	Blumeria graminis f. sp	143:165	Differential accumulation of callose, arabinoxylan and cellulose in nonpenetrated versus penetrated papillae on leaves of barley infected with Blumeria graminis f. sp.
25138067	5	52	theme	barley	712:717	arg1	composition					685:695	the composition	681:695	the composition of papillae of barley (Hordeum vulgare) attacked by the powdery mildew fungus Blumeria graminis f. sp	681:797	Here, we have re-examined the composition of papillae of barley (Hordeum vulgare) attacked by the powdery mildew fungus Blumeria graminis f. sp.
25138067	2	53	theme	major	312:316	arg1	factor					318:323	a major factor	310:323	a major factor in basal host resistance	310:348	In plants, cell walls are one of the first lines of defence for protecting cells from successful invasion by fungal pathogens and are a major factor in basal host resistance.
25138067	10	54	theme	new	1452:1454	arg1	targets					1456:1462	new targets	1452:1462	new targets for the improvement of papilla composition and enhanced disease resistance	1452:1537	The association of arabinoxylan and cellulose with penetration resistance suggests new targets for the improvement of papilla composition and enhanced disease resistance.
25138067	0	55	theme	graminis	152:159	arg1	sp					164:165	Blumeria graminis f. sp	143:165	Blumeria graminis f. sp	143:165	Differential accumulation of callose, arabinoxylan and cellulose in nonpenetrated versus penetrated papillae on leaves of barley infected with Blumeria graminis f. sp.
25138067	6	56	theme	antibodies	830:839	arg1	range					821:825	a range	819:825	a range of antibodies and carbohydrate-binding modules that are targeted to cell wall polysaccharides	819:919	hordei (Bgh) using a range of antibodies and carbohydrate-binding modules that are targeted to cell wall polysaccharides.
25138067	8	57	located	found	1147:1151	arg2	concentrations					1089:1102	Higher concentrations	1082:1102	Higher concentrations of callose, arabinoxylan and cellulose	1082:1141	Higher concentrations of callose, arabinoxylan and cellulose are found in effective papillae, compared with ineffective papillae.
25138067	8	57	located	found	1147:1151	arg1	papillae					1166:1173	effective papillae	1156:1173	effective papillae	1156:1173	Higher concentrations of callose, arabinoxylan and cellulose are found in effective papillae, compared with ineffective papillae.
25138067	10	58	theme	arabinoxylan	1388:1399	arg1	association					1373:1383	The association	1369:1383	The association of arabinoxylan and cellulose with penetration resistance	1369:1441	The association of arabinoxylan and cellulose with penetration resistance suggests new targets for the improvement of papilla composition and enhanced disease resistance.
25138067	5	59	dep	graminis	784:791	arg1	sp					796:797	f. sp	793:797	the powdery mildew fungus Blumeria graminis f. sp	749:797	Here, we have re-examined the composition of papillae of barley (Hordeum vulgare) attacked by the powdery mildew fungus Blumeria graminis f. sp.
25138067	0	60	from	leaves	112:117	arg1	accumulation					13:24	Differential accumulation	0:24	Differential accumulation of callose, arabinoxylan and cellulose in nonpenetrated versus penetrated papillae on leaves of barley	0:127	Differential accumulation of callose, arabinoxylan and cellulose in nonpenetrated versus penetrated papillae on leaves of barley infected with Blumeria graminis f. sp.
25138067	7	61	theme	barley	941:946	arg1	papillae					948:955	barley papillae	941:955	barley papillae induced during infection with Bgh	941:989	The data show that barley papillae induced during infection with Bgh contain, in addition to callose, significant concentrations of cellulose and arabinoxylan.
25138067	2	62	theme	cell	187:190	arg1	walls					192:196	cell walls	187:196	cell walls	187:196	In plants, cell walls are one of the first lines of defence for protecting cells from successful invasion by fungal pathogens and are a major factor in basal host resistance.
25138067	2	63	theme	defence	228:234	arg1	lines					219:223	the first lines	209:223	the first lines of defence for protecting cells from successful invasion by fungal pathogens	209:300	In plants, cell walls are one of the first lines of defence for protecting cells from successful invasion by fungal pathogens and are a major factor in basal host resistance.
25138067	4	64	theme	polysaccharide	629:642	arg1	component					644:652	the main polysaccharide component	620:652	the main polysaccharide component	620:652	Papillae that have been effective in preventing penetration by pathogens are traditionally believed to contain callose as the main polysaccharide component.
25138067	4	64	theme	polysaccharide	629:642	arg1	callose					609:615	callose	609:615	callose	609:615	Papillae that have been effective in preventing penetration by pathogens are traditionally believed to contain callose as the main polysaccharide component.
25138067	0	65	theme	nonpenetrated	68:80	arg1	papillae					100:107	nonpenetrated versus penetrated papillae	68:107	nonpenetrated versus penetrated papillae on leaves of barley	68:127	Differential accumulation of callose, arabinoxylan and cellulose in nonpenetrated versus penetrated papillae on leaves of barley infected with Blumeria graminis f. sp.
25138067	0	66	from	papillae	100:107	arg1	leaves					112:117	leaves	112:117	leaves	112:117	Differential accumulation of callose, arabinoxylan and cellulose in nonpenetrated versus penetrated papillae on leaves of barley infected with Blumeria graminis f. sp.
25138067	2	67	theme	host	334:337	arg1	resistance					339:348	basal host resistance	328:348	basal host resistance	328:348	In plants, cell walls are one of the first lines of defence for protecting cells from successful invasion by fungal pathogens and are a major factor in basal host resistance.
25138067	10	68	with	association	1373:1383	arg1	resistance					1432:1441	penetration resistance	1420:1441	penetration resistance	1420:1441	The association of arabinoxylan and cellulose with penetration resistance suggests new targets for the improvement of papilla composition and enhanced disease resistance.
25138067	3	69	theme	plant	359:363	arg1	cell					365:368	the plant cell	355:368	the plant cell	355:368	For the plant cell to block penetration attempts, it must adapt its cell wall to withstand the physical and chemical forces applied by the fungus.
25138067	6	70	theme	carbohydrate-binding	845:864	arg1	modules					866:872	carbohydrate-binding modules	845:872	carbohydrate-binding modules	845:872	hordei (Bgh) using a range of antibodies and carbohydrate-binding modules that are targeted to cell wall polysaccharides.
25138067	2	71	theme	lines	219:223	arg1	one					202:204	one	202:204	one	202:204	In plants, cell walls are one of the first lines of defence for protecting cells from successful invasion by fungal pathogens and are a major factor in basal host resistance.
25138067	2	71	theme	lines	219:223	arg1	lines					219:223	the first lines	209:223	the first lines of defence for protecting cells from successful invasion by fungal pathogens	209:300	In plants, cell walls are one of the first lines of defence for protecting cells from successful invasion by fungal pathogens and are a major factor in basal host resistance.
25138067	2	72	theme	basal	328:332	arg1	resistance					339:348	basal host resistance	328:348	basal host resistance	328:348	In plants, cell walls are one of the first lines of defence for protecting cells from successful invasion by fungal pathogens and are a major factor in basal host resistance.
25138067	9	73	contain	have	1225:1228	arg1	papillae					1216:1223	The papillae	1212:1223	The papillae	1212:1223	The papillae have a layered structure, with the inner core consisting of callose and arabinoxylan and the outer layer containing arabinoxylan and cellulose.
25138067	9	73	contain	have	1225:1228	arg2	structure					1240:1248	a layered structure	1230:1248	a layered structure	1230:1248	The papillae have a layered structure, with the inner core consisting of callose and arabinoxylan and the outer layer containing arabinoxylan and cellulose.
24694253	5	0	theme	thin	627:630	arg1	film					638:641	a thin solid film	625:641	a thin solid film	625:641	When dried into a thin solid film, the CNCs retain the helicoidal chiral nematic order and assemble into a layered structure where the CNCs have aligned orientation within each layer, and their orientation rotates through the stack with a characteristic pitch (repeating distance).
24694253	17	1	theme	organosilica	2433:2444	arg1	materials					2446:2454	organosilica materials	2433:2454	organosilica materials	2433:2454	Chiral nematic mesoporous silica and organosilica materials, obtainable as centimeter-scale freestanding films, are interesting hosts for nanomaterials.
24694253	19	2	theme	circular	2833:2840	arg1	spectra					2852:2858	induced circular dichroism spectra	2825:2858	induced circular dichroism spectra when encapsulated in the chiral nematic host	2825:2903	Fluorescent conjugated polymers show induced circular dichroism spectra when encapsulated in the chiral nematic host.
24694253	18	3	theme	surface	2679:2685	arg1	resonances					2695:2704	their surface plasmon resonances	2673:2704	their surface plasmon resonances that arise from dipolar coupling of the particles within the chiral nematic host	2673:2785	When noble metal nanoparticles are incorporated into the pores, they show strong circular dichroism signals associated with their surface plasmon resonances that arise from dipolar coupling of the particles within the chiral nematic host.
24694253	19	4	theme	induced	2825:2831	arg1	spectra					2852:2858	induced circular dichroism spectra	2825:2858	induced circular dichroism spectra when encapsulated in the chiral nematic host	2825:2903	Fluorescent conjugated polymers show induced circular dichroism spectra when encapsulated in the chiral nematic host.
24694253	21	5	dep	mesoporous	3059:3068	arg1	silica					3070:3075	silica	3070:3075	silica	3070:3075	We describe the development of chiral nematic mesoporous silica and organosilica, demonstrate different avenues of host-guest chemistry, and identify future directions that exploit the unique combination of properties present in these materials.
24694253	21	5	dep	mesoporous	3059:3068	arg1	mesoporous					3059:3068	chiral nematic mesoporous silica and organosilica	3044:3092	chiral nematic mesoporous silica and organosilica	3044:3092	We describe the development of chiral nematic mesoporous silica and organosilica, demonstrate different avenues of host-guest chemistry, and identify future directions that exploit the unique combination of properties present in these materials.
24694253	21	5	dep	mesoporous	3059:3068	arg1	organosilica					3081:3092	organosilica	3081:3092	organosilica	3081:3092	We describe the development of chiral nematic mesoporous silica and organosilica, demonstrate different avenues of host-guest chemistry, and identify future directions that exploit the unique combination of properties present in these materials.
24694253	14	6	theme	films	2074:2078	arg1	composition					2044:2054	The composition	2040:2054	The composition of the mesoporous films	2040:2078	The composition of the mesoporous films can be varied by using assorted organosilica precursors.
24694253	7	7	theme	CNC	1059:1061	arg1	self-assembly					1063:1075	CNC self-assembly	1059:1075	CNC self-assembly	1059:1075	During CNC self-assembly, it is possible to add sol-gel precursors, such as Si(OMe)4, that undergo hydrolysis and condensation as the solvent evaporates, leading to a chiral nematic silica/CNC composite material.
24694253	18	8	theme	dipolar	2722:2728	arg1	coupling					2730:2737	dipolar coupling	2722:2737	dipolar coupling of the particles within the chiral nematic host	2722:2785	When noble metal nanoparticles are incorporated into the pores, they show strong circular dichroism signals associated with their surface plasmon resonances that arise from dipolar coupling of the particles within the chiral nematic host.
24694253	2	9	theme	cellulose	251:259	arg1	source					261:266	the cellulose source	247:266	the cellulose source	247:266	The nanocrystals have diameters of ~5-15 nm and lengths of ~100-300 nm (depending on the cellulose source and hydrolysis conditions).
24694253	22	10	theme	composite	3342:3350	arg1	materials					3352:3360	composite materials	3342:3360	composite materials accessible using CNC templating	3342:3392	The examples covered in this Account demonstrate that there is a rich diversity of composite materials accessible using CNC templating.
24694253	11	11	theme	chiral	1694:1699	arg1	order					1709:1713	chiral nematic order	1694:1713	chiral nematic order	1694:1713	In this Account, we describe our recent research into mesoporous films with chiral nematic order.
24694253	17	12	dep	mesoporous	2411:2420	arg1	silica					2422:2427	silica	2422:2427	silica	2422:2427	Chiral nematic mesoporous silica and organosilica materials, obtainable as centimeter-scale freestanding films, are interesting hosts for nanomaterials.
24694253	17	12	dep	mesoporous	2411:2420	arg1	materials					2446:2454	organosilica materials	2433:2454	organosilica materials	2433:2454	Chiral nematic mesoporous silica and organosilica materials, obtainable as centimeter-scale freestanding films, are interesting hosts for nanomaterials.
24694253	21	13	from	materials	3248:3256	arg1	present					3231:3237	present	3231:3237	present	3231:3237	We describe the development of chiral nematic mesoporous silica and organosilica, demonstrate different avenues of host-guest chemistry, and identify future directions that exploit the unique combination of properties present in these materials.
24694253	5	14	theme	helicoidal	664:673	arg1	order					690:694	the helicoidal chiral nematic order	660:694	the helicoidal chiral nematic order	660:694	When dried into a thin solid film, the CNCs retain the helicoidal chiral nematic order and assemble into a layered structure where the CNCs have aligned orientation within each layer, and their orientation rotates through the stack with a characteristic pitch (repeating distance).
24694253	1	15	theme	sulfuric	108:115	arg1	hydrolysis					132:141	the sulfuric acid-catalyzed hydrolysis	104:141	the sulfuric acid-catalyzed hydrolysis of bulk cellulose	104:159	Cellulose nanocrystals (CNCs) are obtained from the sulfuric acid-catalyzed hydrolysis of bulk cellulose.
24694253	8	16	theme	area	1356:1359	arg1	film					1379:1382	a high surface area mesoporous silica film	1341:1382	a high surface area mesoporous silica film that has pore diameters of ~3-10 nm	1341:1418	Calcination of the material in air destroys the cellulose template, leaving a high surface area mesoporous silica film that has pore diameters of ~3-10 nm.
24694253	19	17	theme	nematic	2892:2898	arg1	host					2900:2903	the chiral nematic host	2881:2903	the chiral nematic host	2881:2903	Fluorescent conjugated polymers show induced circular dichroism spectra when encapsulated in the chiral nematic host.
24694253	5	18	theme	nematic	682:688	arg1	order					690:694	the helicoidal chiral nematic order	660:694	the helicoidal chiral nematic order	660:694	When dried into a thin solid film, the CNCs retain the helicoidal chiral nematic order and assemble into a layered structure where the CNCs have aligned orientation within each layer, and their orientation rotates through the stack with a characteristic pitch (repeating distance).
24694253	6	19	theme	cholesteric	895:905	arg1	structure					942:950	a 1-D photonic structure	927:950	a 1-D photonic structure	927:950	The cholesteric ordering can act as a 1-D photonic structure, selectively reflecting circularly polarized light that has a wavelength nearly matching the pitch.
24694253	6	19	theme	cholesteric	895:905	arg1	ordering					907:914	The cholesteric ordering	891:914	The cholesteric ordering	891:914	The cholesteric ordering can act as a 1-D photonic structure, selectively reflecting circularly polarized light that has a wavelength nearly matching the pitch.
24694253	8	20	theme	silica	1372:1377	arg1	film					1379:1382	a high surface area mesoporous silica film	1341:1382	a high surface area mesoporous silica film that has pore diameters of ~3-10 nm	1341:1418	Calcination of the material in air destroys the cellulose template, leaving a high surface area mesoporous silica film that has pore diameters of ~3-10 nm.
24694253	17	21	theme	interesting	2512:2522	arg1	hosts					2524:2528	interesting hosts	2512:2528	interesting hosts for nanomaterials	2512:2546	Chiral nematic mesoporous silica and organosilica materials, obtainable as centimeter-scale freestanding films, are interesting hosts for nanomaterials.
24694253	17	21	theme	interesting	2512:2522	arg1	mesoporous					2411:2420	Chiral nematic mesoporous silica and organosilica materials	2396:2454	Chiral nematic mesoporous silica and organosilica materials	2396:2454	Chiral nematic mesoporous silica and organosilica materials, obtainable as centimeter-scale freestanding films, are interesting hosts for nanomaterials.
24694253	13	22	theme	mesoporous	1969:1978	arg1	films					1987:1991	mesoporous carbon films	1969:1991	mesoporous carbon films that can be used as supercapacitor electrodes	1969:2037	For example, heating the silica/CNC composites under an inert atmosphere followed by removal of the silica leaves highly ordered, mesoporous carbon films that can be used as supercapacitor electrodes.
24694253	13	22	theme	mesoporous	1969:1978	arg1	electrodes					2028:2037	supercapacitor electrodes	2013:2037	supercapacitor electrodes	2013:2037	For example, heating the silica/CNC composites under an inert atmosphere followed by removal of the silica leaves highly ordered, mesoporous carbon films that can be used as supercapacitor electrodes.
24694253	5	23	theme	repeating	870:878	arg1	pitch					863:867	a characteristic pitch	846:867	a characteristic pitch (repeating distance)	846:888	When dried into a thin solid film, the CNCs retain the helicoidal chiral nematic order and assemble into a layered structure where the CNCs have aligned orientation within each layer, and their orientation rotates through the stack with a characteristic pitch (repeating distance).
24694253	5	23	theme	repeating	870:878	arg1	distance					880:887	repeating distance	870:887	repeating distance	870:887	When dried into a thin solid film, the CNCs retain the helicoidal chiral nematic order and assemble into a layered structure where the CNCs have aligned orientation within each layer, and their orientation rotates through the stack with a characteristic pitch (repeating distance).
24694253	18	24	theme	strong	2623:2628	arg1	signals					2649:2655	strong circular dichroism signals	2623:2655	strong circular dichroism signals associated with their surface plasmon resonances that arise from dipolar coupling of the particles within the chiral nematic host	2623:2785	When noble metal nanoparticles are incorporated into the pores, they show strong circular dichroism signals associated with their surface plasmon resonances that arise from dipolar coupling of the particles within the chiral nematic host.
24694253	4	25	dep	crystal	591:597	arg1	referred					556:563	referred	556:563	referred to as cholesteric	556:581	But CNCs have an additional, less explored property: they organize into a chiral nematic (historically referred to as cholesteric) liquid crystal in water.
24694253	8	26	theme	pore	1393:1396	arg1	diameters					1398:1406	pore diameters	1393:1406	pore diameters of ~3-10 nm	1393:1418	Calcination of the material in air destroys the cellulose template, leaving a high surface area mesoporous silica film that has pore diameters of ~3-10 nm.
24694253	5	27	theme	layered	716:722	arg1	structure					724:732	a layered structure	714:732	a layered structure where the CNCs have aligned orientation within each layer	714:790	When dried into a thin solid film, the CNCs retain the helicoidal chiral nematic order and assemble into a layered structure where the CNCs have aligned orientation within each layer, and their orientation rotates through the stack with a characteristic pitch (repeating distance).
24694253	21	28	theme	nematic	3051:3057	arg1	silica					3070:3075	silica	3070:3075	silica	3070:3075	We describe the development of chiral nematic mesoporous silica and organosilica, demonstrate different avenues of host-guest chemistry, and identify future directions that exploit the unique combination of properties present in these materials.
24694253	21	28	theme	nematic	3051:3057	arg1	mesoporous					3059:3068	chiral nematic mesoporous silica and organosilica	3044:3092	chiral nematic mesoporous silica and organosilica	3044:3092	We describe the development of chiral nematic mesoporous silica and organosilica, demonstrate different avenues of host-guest chemistry, and identify future directions that exploit the unique combination of properties present in these materials.
24694253	21	28	theme	nematic	3051:3057	arg1	organosilica					3081:3092	organosilica	3081:3092	organosilica	3081:3092	We describe the development of chiral nematic mesoporous silica and organosilica, demonstrate different avenues of host-guest chemistry, and identify future directions that exploit the unique combination of properties present in these materials.
24694253	1	29	theme	bulk	146:149	arg1	cellulose					151:159	bulk cellulose	146:159	bulk cellulose	146:159	Cellulose nanocrystals (CNCs) are obtained from the sulfuric acid-catalyzed hydrolysis of bulk cellulose.
24694253	3	30	theme	lightweight	301:311	arg1	material					313:320	This lightweight material	296:320	This lightweight material	296:320	This lightweight material has mostly been investigated to reinforce composites and polymers because it has remarkable strength that rivals carbon nanotubes.
24694253	12	31	theme	new	1797:1799	arg1	materials					1812:1820	new functional materials	1797:1820	new functional materials	1797:1820	Taking advantage of the chiral nematic order and nanoscale of the CNC templates, new functional materials can be prepared.
24694253	8	32	theme	nm	1417:1418	arg1	diameters					1398:1406	pore diameters	1393:1406	pore diameters of ~3-10 nm	1393:1418	Calcination of the material in air destroys the cellulose template, leaving a high surface area mesoporous silica film that has pore diameters of ~3-10 nm.
24694253	12	33	dep	prepared	1829:1836	arg1	nanoscale					1765:1773	nanoscale	1765:1773	nanoscale	1765:1773	Taking advantage of the chiral nematic order and nanoscale of the CNC templates, new functional materials can be prepared.
24694253	12	33	dep	prepared	1829:1836	arg1	Taking					1716:1721	Taking	1716:1721	Taking advantage of the chiral nematic order	1716:1759	Taking advantage of the chiral nematic order and nanoscale of the CNC templates, new functional materials can be prepared.
24694253	18	34	theme	dichroism	2639:2647	arg1	signals					2649:2655	strong circular dichroism signals	2623:2655	strong circular dichroism signals associated with their surface plasmon resonances that arise from dipolar coupling of the particles within the chiral nematic host	2623:2785	When noble metal nanoparticles are incorporated into the pores, they show strong circular dichroism signals associated with their surface plasmon resonances that arise from dipolar coupling of the particles within the chiral nematic host.
24694253	11	35	theme	recent	1651:1656	arg1	research					1658:1665	our recent research	1647:1665	our recent research into mesoporous films with chiral nematic order	1647:1713	In this Account, we describe our recent research into mesoporous films with chiral nematic order.
24694253	4	36	dep	referred	556:563	arg1	historically					543:554	historically	543:554	historically	543:554	But CNCs have an additional, less explored property: they organize into a chiral nematic (historically referred to as cholesteric) liquid crystal in water.
24694253	6	37	theme	1-D	929:931	arg1	structure					942:950	a 1-D photonic structure	927:950	a 1-D photonic structure	927:950	The cholesteric ordering can act as a 1-D photonic structure, selectively reflecting circularly polarized light that has a wavelength nearly matching the pitch.
24694253	6	37	theme	1-D	929:931	arg1	ordering					907:914	The cholesteric ordering	891:914	The cholesteric ordering	891:914	The cholesteric ordering can act as a 1-D photonic structure, selectively reflecting circularly polarized light that has a wavelength nearly matching the pitch.
24694253	3	38	theme	carbon	435:440	arg1	nanotubes					442:450	carbon nanotubes	435:450	carbon nanotubes	435:450	This lightweight material has mostly been investigated to reinforce composites and polymers because it has remarkable strength that rivals carbon nanotubes.
24694253	11	39	with	films	1683:1687	arg1	order					1709:1713	chiral nematic order	1694:1713	chiral nematic order	1694:1713	In this Account, we describe our recent research into mesoporous films with chiral nematic order.
24694253	17	40	theme	freestanding	2488:2499	arg1	films					2501:2505	centimeter-scale freestanding films	2471:2505	centimeter-scale freestanding films	2471:2505	Chiral nematic mesoporous silica and organosilica materials, obtainable as centimeter-scale freestanding films, are interesting hosts for nanomaterials.
24694253	18	41	theme	metal	2560:2564	arg1	nanoparticles					2566:2578	noble metal nanoparticles	2554:2578	noble metal nanoparticles	2554:2578	When noble metal nanoparticles are incorporated into the pores, they show strong circular dichroism signals associated with their surface plasmon resonances that arise from dipolar coupling of the particles within the chiral nematic host.
24694253	21	42	theme	chemistry	3139:3147	arg1	avenues					3117:3123	different avenues	3107:3123	different avenues of host-guest chemistry	3107:3147	We describe the development of chiral nematic mesoporous silica and organosilica, demonstrate different avenues of host-guest chemistry, and identify future directions that exploit the unique combination of properties present in these materials.
24694253	15	43	theme	cellulose	2158:2166	arg1	removal					2143:2149	removal	2143:2149	removal of the cellulose by acid-catalyzed hydrolysis	2143:2195	After removal of the cellulose by acid-catalyzed hydrolysis, highly porous, iridescent organosilica films are obtained.
24694253	12	44	theme	templates	1786:1794	arg1	nanoscale					1765:1773	nanoscale	1765:1773	nanoscale	1765:1773	Taking advantage of the chiral nematic order and nanoscale of the CNC templates, new functional materials can be prepared.
24694253	7	45	theme	silica/CNC	1234:1243	arg1	material					1255:1262	a chiral nematic silica/CNC composite material	1217:1262	a chiral nematic silica/CNC composite material	1217:1262	During CNC self-assembly, it is possible to add sol-gel precursors, such as Si(OMe)4, that undergo hydrolysis and condensation as the solvent evaporates, leading to a chiral nematic silica/CNC composite material.
24694253	4	46	theme	nematic	534:540	arg1	crystal					591:597	a chiral nematic (historically referred to as cholesteric) liquid crystal	525:597	a chiral nematic (historically referred to as cholesteric) liquid crystal in water	525:606	But CNCs have an additional, less explored property: they organize into a chiral nematic (historically referred to as cholesteric) liquid crystal in water.
24694253	4	47	dep	additional	470:479	arg1	explored					487:494	explored	487:494	explored	487:494	But CNCs have an additional, less explored property: they organize into a chiral nematic (historically referred to as cholesteric) liquid crystal in water.
24694253	12	48	theme	order	1755:1759	arg1	advantage					1723:1731	advantage	1723:1731	advantage of the chiral nematic order	1723:1759	Taking advantage of the chiral nematic order and nanoscale of the CNC templates, new functional materials can be prepared.
24694253	15	49	dep	porous	2205:2210	arg1	iridescent					2213:2222	iridescent	2213:2222	iridescent	2213:2222	After removal of the cellulose by acid-catalyzed hydrolysis, highly porous, iridescent organosilica films are obtained.
24694253	7	50	theme	chiral	1219:1224	arg1	material					1255:1262	a chiral nematic silica/CNC composite material	1217:1262	a chiral nematic silica/CNC composite material	1217:1262	During CNC self-assembly, it is possible to add sol-gel precursors, such as Si(OMe)4, that undergo hydrolysis and condensation as the solvent evaporates, leading to a chiral nematic silica/CNC composite material.
24694253	3	51	theme	remarkable	403:412	arg1	strength					414:421	remarkable strength	403:421	remarkable strength that rivals carbon nanotubes	403:450	This lightweight material has mostly been investigated to reinforce composites and polymers because it has remarkable strength that rivals carbon nanotubes.
24694253	21	52	theme	different	3107:3115	arg1	avenues					3117:3123	different avenues	3107:3123	different avenues of host-guest chemistry	3107:3147	We describe the development of chiral nematic mesoporous silica and organosilica, demonstrate different avenues of host-guest chemistry, and identify future directions that exploit the unique combination of properties present in these materials.
24694253	8	53	theme	cellulose	1313:1321	arg1	template					1323:1330	the cellulose template	1309:1330	the cellulose template	1309:1330	Calcination of the material in air destroys the cellulose template, leaving a high surface area mesoporous silica film that has pore diameters of ~3-10 nm.
24694253	10	54	theme	optical	1574:1580	arg1	filters					1582:1588	optical filters	1574:1588	optical filters	1574:1588	These films may be useful as optical filters, reflectors, and membranes.
24694253	7	55	theme	sol-gel	1100:1106	arg1	OMe					1131:1133	Si(OMe)4	1128:1135	Si(OMe)4	1128:1135	During CNC self-assembly, it is possible to add sol-gel precursors, such as Si(OMe)4, that undergo hydrolysis and condensation as the solvent evaporates, leading to a chiral nematic silica/CNC composite material.
24694253	7	55	theme	sol-gel	1100:1106	arg1	precursors					1108:1117	sol-gel precursors	1100:1117	sol-gel precursors	1100:1117	During CNC self-assembly, it is possible to add sol-gel precursors, such as Si(OMe)4, that undergo hydrolysis and condensation as the solvent evaporates, leading to a chiral nematic silica/CNC composite material.
24694253	0	56	theme	nematic	26:32	arg1	materials					45:53	chiral nematic mesoporous materials	19:53	chiral nematic mesoporous materials	19:53	The development of chiral nematic mesoporous materials.
24694253	21	57	theme	present	3231:3237	arg1	properties					3220:3229	properties	3220:3229	properties present in these materials	3220:3256	We describe the development of chiral nematic mesoporous silica and organosilica, demonstrate different avenues of host-guest chemistry, and identify future directions that exploit the unique combination of properties present in these materials.
24694253	0	58	theme	materials	45:53	arg1	development					4:14	The development	0:14	The development of chiral nematic mesoporous materials	0:53	The development of chiral nematic mesoporous materials.
24694253	9	59	theme	chiral	1519:1524	arg1	structure					1534:1542	the chiral nematic structure	1515:1542	the chiral nematic structure	1515:1542	Importantly, the silica is brilliantly iridescent because the pores in its interior replicate the chiral nematic structure.
24694253	2	60	theme	nm	203:204	arg1	lengths					210:216	lengths	210:216	lengths of ~100-300 nm	210:231	The nanocrystals have diameters of ~5-15 nm and lengths of ~100-300 nm (depending on the cellulose source and hydrolysis conditions).
24694253	2	60	theme	nm	203:204	arg1	diameters					184:192	diameters	184:192	diameters of ~5-15 nm	184:204	The nanocrystals have diameters of ~5-15 nm and lengths of ~100-300 nm (depending on the cellulose source and hydrolysis conditions).
24694253	12	61	theme	chiral	1740:1745	arg1	order					1755:1759	the chiral nematic order	1736:1759	the chiral nematic order	1736:1759	Taking advantage of the chiral nematic order and nanoscale of the CNC templates, new functional materials can be prepared.
24694253	18	62	theme	particles	2746:2754	arg1	coupling					2730:2737	dipolar coupling	2722:2737	dipolar coupling of the particles within the chiral nematic host	2722:2785	When noble metal nanoparticles are incorporated into the pores, they show strong circular dichroism signals associated with their surface plasmon resonances that arise from dipolar coupling of the particles within the chiral nematic host.
24694253	7	63	theme	solvent	1186:1192	arg1	condensation					1166:1177	condensation	1166:1177	condensation	1166:1177	During CNC self-assembly, it is possible to add sol-gel precursors, such as Si(OMe)4, that undergo hydrolysis and condensation as the solvent evaporates, leading to a chiral nematic silica/CNC composite material.
24694253	7	63	theme	solvent	1186:1192	arg1	hydrolysis					1151:1160	hydrolysis	1151:1160	hydrolysis	1151:1160	During CNC self-assembly, it is possible to add sol-gel precursors, such as Si(OMe)4, that undergo hydrolysis and condensation as the solvent evaporates, leading to a chiral nematic silica/CNC composite material.
24694253	7	63	theme	solvent	1186:1192	arg1	evaporates					1194:1203	the solvent evaporates	1182:1203	the solvent evaporates	1182:1203	During CNC self-assembly, it is possible to add sol-gel precursors, such as Si(OMe)4, that undergo hydrolysis and condensation as the solvent evaporates, leading to a chiral nematic silica/CNC composite material.
24694253	17	64	theme	Chiral	2396:2401	arg1	hosts					2524:2528	interesting hosts	2512:2528	interesting hosts for nanomaterials	2512:2546	Chiral nematic mesoporous silica and organosilica materials, obtainable as centimeter-scale freestanding films, are interesting hosts for nanomaterials.
24694253	17	64	theme	Chiral	2396:2401	arg1	mesoporous					2411:2420	Chiral nematic mesoporous silica and organosilica materials	2396:2454	Chiral nematic mesoporous silica and organosilica materials	2396:2454	Chiral nematic mesoporous silica and organosilica materials, obtainable as centimeter-scale freestanding films, are interesting hosts for nanomaterials.
24694253	4	65	theme	liquid	584:589	arg1	crystal					591:597	a chiral nematic (historically referred to as cholesteric) liquid crystal	525:597	a chiral nematic (historically referred to as cholesteric) liquid crystal in water	525:606	But CNCs have an additional, less explored property: they organize into a chiral nematic (historically referred to as cholesteric) liquid crystal in water.
24694253	14	66	theme	organosilica	2112:2123	arg1	precursors					2125:2134	assorted organosilica precursors	2103:2134	assorted organosilica precursors	2103:2134	The composition of the mesoporous films can be varied by using assorted organosilica precursors.
24694253	8	67	theme	surface	1348:1354	arg1	film					1379:1382	a high surface area mesoporous silica film	1341:1382	a high surface area mesoporous silica film that has pore diameters of ~3-10 nm	1341:1418	Calcination of the material in air destroys the cellulose template, leaving a high surface area mesoporous silica film that has pore diameters of ~3-10 nm.
24694253	18	68	theme	chiral	2767:2772	arg1	host					2782:2785	the chiral nematic host	2763:2785	the chiral nematic host	2763:2785	When noble metal nanoparticles are incorporated into the pores, they show strong circular dichroism signals associated with their surface plasmon resonances that arise from dipolar coupling of the particles within the chiral nematic host.
24694253	15	69	theme	porous	2205:2210	arg1	films					2237:2241	highly porous, iridescent organosilica films	2198:2241	highly porous, iridescent organosilica films	2198:2241	After removal of the cellulose by acid-catalyzed hydrolysis, highly porous, iridescent organosilica films are obtained.
24694253	16	70	theme	mechanical	2333:2342	arg1	properties					2344:2353	the chemical and mechanical properties	2316:2353	the chemical and mechanical properties	2316:2353	These materials are flexible and offer the ability to tune the chemical and mechanical properties through variation of the organic spacer.
24694253	21	71	theme	future	3163:3168	arg1	directions					3170:3179	future directions	3163:3179	future directions that exploit the unique combination of properties present in these materials	3163:3256	We describe the development of chiral nematic mesoporous silica and organosilica, demonstrate different avenues of host-guest chemistry, and identify future directions that exploit the unique combination of properties present in these materials.
24694253	13	72	theme	supercapacitor	2013:2026	arg1	films					1987:1991	mesoporous carbon films	1969:1991	mesoporous carbon films that can be used as supercapacitor electrodes	1969:2037	For example, heating the silica/CNC composites under an inert atmosphere followed by removal of the silica leaves highly ordered, mesoporous carbon films that can be used as supercapacitor electrodes.
24694253	13	72	theme	supercapacitor	2013:2026	arg1	electrodes					2028:2037	supercapacitor electrodes	2013:2037	supercapacitor electrodes	2013:2037	For example, heating the silica/CNC composites under an inert atmosphere followed by removal of the silica leaves highly ordered, mesoporous carbon films that can be used as supercapacitor electrodes.
24694253	3	73	contain	has	399:401	arg2	strength					414:421	remarkable strength	403:421	remarkable strength that rivals carbon nanotubes	403:450	This lightweight material has mostly been investigated to reinforce composites and polymers because it has remarkable strength that rivals carbon nanotubes.
24694253	3	73	contain	has	399:401	arg1	it					396:397	it	396:397	it	396:397	This lightweight material has mostly been investigated to reinforce composites and polymers because it has remarkable strength that rivals carbon nanotubes.
24694253	18	74	theme	noble	2554:2558	arg1	nanoparticles					2566:2578	noble metal nanoparticles	2554:2578	noble metal nanoparticles	2554:2578	When noble metal nanoparticles are incorporated into the pores, they show strong circular dichroism signals associated with their surface plasmon resonances that arise from dipolar coupling of the particles within the chiral nematic host.
24694253	5	75	theme	solid	632:636	arg1	film					638:641	a thin solid film	625:641	a thin solid film	625:641	When dried into a thin solid film, the CNCs retain the helicoidal chiral nematic order and assemble into a layered structure where the CNCs have aligned orientation within each layer, and their orientation rotates through the stack with a characteristic pitch (repeating distance).
24694253	18	76	theme	plasmon	2687:2693	arg1	resonances					2695:2704	their surface plasmon resonances	2673:2704	their surface plasmon resonances that arise from dipolar coupling of the particles within the chiral nematic host	2673:2785	When noble metal nanoparticles are incorporated into the pores, they show strong circular dichroism signals associated with their surface plasmon resonances that arise from dipolar coupling of the particles within the chiral nematic host.
24694253	2	77	theme	hydrolysis	272:281	arg1	conditions					283:292	hydrolysis conditions	272:292	hydrolysis conditions	272:292	The nanocrystals have diameters of ~5-15 nm and lengths of ~100-300 nm (depending on the cellulose source and hydrolysis conditions).
24694253	15	78	theme	acid-catalyzed	2171:2184	arg1	hydrolysis					2186:2195	acid-catalyzed hydrolysis	2171:2195	acid-catalyzed hydrolysis	2171:2195	After removal of the cellulose by acid-catalyzed hydrolysis, highly porous, iridescent organosilica films are obtained.
24694253	13	79	used	used	2005:2008	arg2	electrodes					2028:2037	supercapacitor electrodes	2013:2037	supercapacitor electrodes	2013:2037	For example, heating the silica/CNC composites under an inert atmosphere followed by removal of the silica leaves highly ordered, mesoporous carbon films that can be used as supercapacitor electrodes.
24694253	13	79	used	used	2005:2008	arg2	films					1987:1991	mesoporous carbon films	1969:1991	mesoporous carbon films that can be used as supercapacitor electrodes	1969:2037	For example, heating the silica/CNC composites under an inert atmosphere followed by removal of the silica leaves highly ordered, mesoporous carbon films that can be used as supercapacitor electrodes.
24694253	5	80	theme	characteristic	848:861	arg1	pitch					863:867	a characteristic pitch	846:867	a characteristic pitch (repeating distance)	846:888	When dried into a thin solid film, the CNCs retain the helicoidal chiral nematic order and assemble into a layered structure where the CNCs have aligned orientation within each layer, and their orientation rotates through the stack with a characteristic pitch (repeating distance).
24694253	5	80	theme	characteristic	848:861	arg1	distance					880:887	repeating distance	870:887	repeating distance	870:887	When dried into a thin solid film, the CNCs retain the helicoidal chiral nematic order and assemble into a layered structure where the CNCs have aligned orientation within each layer, and their orientation rotates through the stack with a characteristic pitch (repeating distance).
24694253	2	81	dep	have	179:182	arg1	source					261:266	the cellulose source	247:266	the cellulose source	247:266	The nanocrystals have diameters of ~5-15 nm and lengths of ~100-300 nm (depending on the cellulose source and hydrolysis conditions).
24694253	2	81	dep	have	179:182	arg1	conditions					283:292	hydrolysis conditions	272:292	hydrolysis conditions	272:292	The nanocrystals have diameters of ~5-15 nm and lengths of ~100-300 nm (depending on the cellulose source and hydrolysis conditions).
24694253	21	82	theme	properties	3220:3229	arg1	combination					3205:3215	the unique combination	3194:3215	the unique combination of properties present in these materials	3194:3256	We describe the development of chiral nematic mesoporous silica and organosilica, demonstrate different avenues of host-guest chemistry, and identify future directions that exploit the unique combination of properties present in these materials.
24694253	22	83	theme	materials	3352:3360	arg1	diversity					3329:3337	a rich diversity	3322:3337	a rich diversity of composite materials accessible using CNC templating	3322:3392	The examples covered in this Account demonstrate that there is a rich diversity of composite materials accessible using CNC templating.
24694253	16	84	theme	organic	2380:2386	arg1	spacer					2388:2393	the organic spacer	2376:2393	the organic spacer	2376:2393	These materials are flexible and offer the ability to tune the chemical and mechanical properties through variation of the organic spacer.
24694253	14	85	theme	mesoporous	2063:2072	arg1	films					2074:2078	the mesoporous films	2059:2078	the mesoporous films	2059:2078	The composition of the mesoporous films can be varied by using assorted organosilica precursors.
24694253	2	86	contain	have	179:182	arg2	lengths					210:216	lengths	210:216	lengths of ~100-300 nm	210:231	The nanocrystals have diameters of ~5-15 nm and lengths of ~100-300 nm (depending on the cellulose source and hydrolysis conditions).
24694253	2	86	contain	have	179:182	arg2	diameters					184:192	diameters	184:192	diameters of ~5-15 nm	184:204	The nanocrystals have diameters of ~5-15 nm and lengths of ~100-300 nm (depending on the cellulose source and hydrolysis conditions).
24694253	2	86	contain	have	179:182	arg1	nanocrystals					166:177	The nanocrystals	162:177	The nanocrystals	162:177	The nanocrystals have diameters of ~5-15 nm and lengths of ~100-300 nm (depending on the cellulose source and hydrolysis conditions).
24694253	20	87	from	use	2997:2999	arg1	sensors					3004:3010	sensors	3004:3010	sensors	3004:3010	The porosity, film structure, and optical properties of these materials could enable their use in sensors.
24694253	19	88	theme	dichroism	2842:2850	arg1	spectra					2852:2858	induced circular dichroism spectra	2825:2858	induced circular dichroism spectra when encapsulated in the chiral nematic host	2825:2903	Fluorescent conjugated polymers show induced circular dichroism spectra when encapsulated in the chiral nematic host.
24694253	11	89	theme	nematic	1701:1707	arg1	order					1709:1713	chiral nematic order	1694:1713	chiral nematic order	1694:1713	In this Account, we describe our recent research into mesoporous films with chiral nematic order.
24694253	22	90	dep	materials	3352:3360	arg1	accessible					3362:3371	accessible	3362:3371	accessible	3362:3371	The examples covered in this Account demonstrate that there is a rich diversity of composite materials accessible using CNC templating.
24694253	22	91	theme	rich	3324:3327	arg1	diversity					3329:3337	a rich diversity	3322:3337	a rich diversity of composite materials accessible using CNC templating	3322:3392	The examples covered in this Account demonstrate that there is a rich diversity of composite materials accessible using CNC templating.
24694253	8	92	theme	mesoporous	1361:1370	arg1	film					1379:1382	a high surface area mesoporous silica film	1341:1382	a high surface area mesoporous silica film that has pore diameters of ~3-10 nm	1341:1418	Calcination of the material in air destroys the cellulose template, leaving a high surface area mesoporous silica film that has pore diameters of ~3-10 nm.
24694253	19	93	theme	chiral	2885:2890	arg1	host					2900:2903	the chiral nematic host	2881:2903	the chiral nematic host	2881:2903	Fluorescent conjugated polymers show induced circular dichroism spectra when encapsulated in the chiral nematic host.
24694253	5	94	theme	chiral	675:680	arg1	order					690:694	the helicoidal chiral nematic order	660:694	the helicoidal chiral nematic order	660:694	When dried into a thin solid film, the CNCs retain the helicoidal chiral nematic order and assemble into a layered structure where the CNCs have aligned orientation within each layer, and their orientation rotates through the stack with a characteristic pitch (repeating distance).
24694253	21	95	from	present	3231:3237	arg1	materials					3248:3256	these materials	3242:3256	these materials	3242:3256	We describe the development of chiral nematic mesoporous silica and organosilica, demonstrate different avenues of host-guest chemistry, and identify future directions that exploit the unique combination of properties present in these materials.
24694253	2	96	theme	nm	230:231	arg1	lengths					210:216	lengths	210:216	lengths of ~100-300 nm	210:231	The nanocrystals have diameters of ~5-15 nm and lengths of ~100-300 nm (depending on the cellulose source and hydrolysis conditions).
24694253	2	96	theme	nm	230:231	arg1	diameters					184:192	diameters	184:192	diameters of ~5-15 nm	184:204	The nanocrystals have diameters of ~5-15 nm and lengths of ~100-300 nm (depending on the cellulose source and hydrolysis conditions).
24694253	18	97	theme	circular	2630:2637	arg1	dichroism					2639:2647	circular dichroism	2630:2647	strong circular dichroism signals associated with their surface plasmon resonances that arise from dipolar coupling of the particles within the chiral nematic host	2623:2785	When noble metal nanoparticles are incorporated into the pores, they show strong circular dichroism signals associated with their surface plasmon resonances that arise from dipolar coupling of the particles within the chiral nematic host.
24694253	13	98	theme	carbon	1980:1985	arg1	films					1987:1991	mesoporous carbon films	1969:1991	mesoporous carbon films that can be used as supercapacitor electrodes	1969:2037	For example, heating the silica/CNC composites under an inert atmosphere followed by removal of the silica leaves highly ordered, mesoporous carbon films that can be used as supercapacitor electrodes.
24694253	13	98	theme	carbon	1980:1985	arg1	electrodes					2028:2037	supercapacitor electrodes	2013:2037	supercapacitor electrodes	2013:2037	For example, heating the silica/CNC composites under an inert atmosphere followed by removal of the silica leaves highly ordered, mesoporous carbon films that can be used as supercapacitor electrodes.
24694253	1	99	theme	acid-catalyzed	117:130	arg1	hydrolysis					132:141	the sulfuric acid-catalyzed hydrolysis	104:141	the sulfuric acid-catalyzed hydrolysis of bulk cellulose	104:159	Cellulose nanocrystals (CNCs) are obtained from the sulfuric acid-catalyzed hydrolysis of bulk cellulose.
24694253	6	100	theme	polarized	987:995	arg1	light					997:1001	circularly polarized light	976:1001	circularly polarized light that has a wavelength nearly matching the pitch	976:1049	The cholesteric ordering can act as a 1-D photonic structure, selectively reflecting circularly polarized light that has a wavelength nearly matching the pitch.
24694253	21	101	theme	chiral	3044:3049	arg1	silica					3070:3075	silica	3070:3075	silica	3070:3075	We describe the development of chiral nematic mesoporous silica and organosilica, demonstrate different avenues of host-guest chemistry, and identify future directions that exploit the unique combination of properties present in these materials.
24694253	21	101	theme	chiral	3044:3049	arg1	mesoporous					3059:3068	chiral nematic mesoporous silica and organosilica	3044:3092	chiral nematic mesoporous silica and organosilica	3044:3092	We describe the development of chiral nematic mesoporous silica and organosilica, demonstrate different avenues of host-guest chemistry, and identify future directions that exploit the unique combination of properties present in these materials.
24694253	21	101	theme	chiral	3044:3049	arg1	organosilica					3081:3092	organosilica	3081:3092	organosilica	3081:3092	We describe the development of chiral nematic mesoporous silica and organosilica, demonstrate different avenues of host-guest chemistry, and identify future directions that exploit the unique combination of properties present in these materials.
24694253	9	102	from	pores	1483:1487	arg1	interior					1496:1503	its interior	1492:1503	its interior	1492:1503	Importantly, the silica is brilliantly iridescent because the pores in its interior replicate the chiral nematic structure.
24694253	4	103	from	crystal	591:597	arg1	water					602:606	water	602:606	water	602:606	But CNCs have an additional, less explored property: they organize into a chiral nematic (historically referred to as cholesteric) liquid crystal in water.
24694253	12	104	theme	functional	1801:1810	arg1	materials					1812:1820	new functional materials	1797:1820	new functional materials	1797:1820	Taking advantage of the chiral nematic order and nanoscale of the CNC templates, new functional materials can be prepared.
24694253	1	105	theme	cellulose	151:159	arg1	hydrolysis					132:141	the sulfuric acid-catalyzed hydrolysis	104:141	the sulfuric acid-catalyzed hydrolysis of bulk cellulose	104:159	Cellulose nanocrystals (CNCs) are obtained from the sulfuric acid-catalyzed hydrolysis of bulk cellulose.
24694253	11	106	theme	mesoporous	1672:1681	arg1	films					1683:1687	mesoporous films	1672:1687	mesoporous films with chiral nematic order	1672:1713	In this Account, we describe our recent research into mesoporous films with chiral nematic order.
24694253	1	107	theme	Cellulose	56:64	arg1	CNCs					80:83	CNCs	80:83	CNCs	80:83	Cellulose nanocrystals (CNCs) are obtained from the sulfuric acid-catalyzed hydrolysis of bulk cellulose.
24694253	1	107	theme	Cellulose	56:64	arg1	nanocrystals					66:77	Cellulose nanocrystals	56:77	Cellulose nanocrystals (CNCs)	56:84	Cellulose nanocrystals (CNCs) are obtained from the sulfuric acid-catalyzed hydrolysis of bulk cellulose.
24694253	6	108	theme	photonic	933:940	arg1	structure					942:950	a 1-D photonic structure	927:950	a 1-D photonic structure	927:950	The cholesteric ordering can act as a 1-D photonic structure, selectively reflecting circularly polarized light that has a wavelength nearly matching the pitch.
24694253	6	108	theme	photonic	933:940	arg1	ordering					907:914	The cholesteric ordering	891:914	The cholesteric ordering	891:914	The cholesteric ordering can act as a 1-D photonic structure, selectively reflecting circularly polarized light that has a wavelength nearly matching the pitch.
24694253	18	109	theme	nematic	2774:2780	arg1	host					2782:2785	the chiral nematic host	2763:2785	the chiral nematic host	2763:2785	When noble metal nanoparticles are incorporated into the pores, they show strong circular dichroism signals associated with their surface plasmon resonances that arise from dipolar coupling of the particles within the chiral nematic host.
24694253	4	110	theme	additional	470:479	arg1	property					496:503	an additional, less explored property	467:503	an additional, less explored property	467:503	But CNCs have an additional, less explored property: they organize into a chiral nematic (historically referred to as cholesteric) liquid crystal in water.
24694253	7	111	theme	Si	1128:1129	arg1	OMe					1131:1133	Si(OMe)4	1128:1135	Si(OMe)4	1128:1135	During CNC self-assembly, it is possible to add sol-gel precursors, such as Si(OMe)4, that undergo hydrolysis and condensation as the solvent evaporates, leading to a chiral nematic silica/CNC composite material.
24694253	12	112	theme	CNC	1782:1784	arg1	templates					1786:1794	the CNC templates	1778:1794	the CNC templates	1778:1794	Taking advantage of the chiral nematic order and nanoscale of the CNC templates, new functional materials can be prepared.
24694253	7	113	theme	composite	1245:1253	arg1	material					1255:1262	a chiral nematic silica/CNC composite material	1217:1262	a chiral nematic silica/CNC composite material	1217:1262	During CNC self-assembly, it is possible to add sol-gel precursors, such as Si(OMe)4, that undergo hydrolysis and condensation as the solvent evaporates, leading to a chiral nematic silica/CNC composite material.
24694253	6	114	contain	has	1008:1010	arg1	light					997:1001	circularly polarized light	976:1001	circularly polarized light that has a wavelength nearly matching the pitch	976:1049	The cholesteric ordering can act as a 1-D photonic structure, selectively reflecting circularly polarized light that has a wavelength nearly matching the pitch.
24694253	6	114	contain	has	1008:1010	arg2	wavelength					1014:1023	a wavelength	1012:1023	a wavelength nearly matching the pitch	1012:1049	The cholesteric ordering can act as a 1-D photonic structure, selectively reflecting circularly polarized light that has a wavelength nearly matching the pitch.
24694253	17	115	theme	centimeter-scale	2471:2486	arg1	films					2501:2505	centimeter-scale freestanding films	2471:2505	centimeter-scale freestanding films	2471:2505	Chiral nematic mesoporous silica and organosilica materials, obtainable as centimeter-scale freestanding films, are interesting hosts for nanomaterials.
24694253	21	116	theme	host-guest	3128:3137	arg1	chemistry					3139:3147	host-guest chemistry	3128:3147	host-guest chemistry	3128:3147	We describe the development of chiral nematic mesoporous silica and organosilica, demonstrate different avenues of host-guest chemistry, and identify future directions that exploit the unique combination of properties present in these materials.
24694253	13	117	theme	silica	1939:1944	arg1	removal					1924:1930	removal	1924:1930	removal of the silica	1924:1944	For example, heating the silica/CNC composites under an inert atmosphere followed by removal of the silica leaves highly ordered, mesoporous carbon films that can be used as supercapacitor electrodes.
24694253	19	118	theme	Fluorescent	2788:2798	arg1	polymers					2811:2818	Fluorescent conjugated polymers	2788:2818	Fluorescent conjugated polymers	2788:2818	Fluorescent conjugated polymers show induced circular dichroism spectra when encapsulated in the chiral nematic host.
24694253	7	119	theme	nematic	1226:1232	arg1	material					1255:1262	a chiral nematic silica/CNC composite material	1217:1262	a chiral nematic silica/CNC composite material	1217:1262	During CNC self-assembly, it is possible to add sol-gel precursors, such as Si(OMe)4, that undergo hydrolysis and condensation as the solvent evaporates, leading to a chiral nematic silica/CNC composite material.
24694253	8	120	theme	material	1284:1291	arg1	Calcination					1265:1275	Calcination	1265:1275	Calcination of the material in air	1265:1298	Calcination of the material in air destroys the cellulose template, leaving a high surface area mesoporous silica film that has pore diameters of ~3-10 nm.
24694253	0	121	theme	chiral	19:24	arg1	materials					45:53	chiral nematic mesoporous materials	19:53	chiral nematic mesoporous materials	19:53	The development of chiral nematic mesoporous materials.
24694253	21	122	attach	present	3231:3237	arg1	materials					3248:3256	these materials	3242:3256	these materials	3242:3256	We describe the development of chiral nematic mesoporous silica and organosilica, demonstrate different avenues of host-guest chemistry, and identify future directions that exploit the unique combination of properties present in these materials.
24694253	21	122	attach	present	3231:3237	arg2	properties					3220:3229	properties	3220:3229	properties present in these materials	3220:3256	We describe the development of chiral nematic mesoporous silica and organosilica, demonstrate different avenues of host-guest chemistry, and identify future directions that exploit the unique combination of properties present in these materials.
24694253	21	123	theme	mesoporous	3059:3068	arg1	development					3029:3039	the development	3025:3039	the development of chiral nematic mesoporous silica and organosilica	3025:3092	We describe the development of chiral nematic mesoporous silica and organosilica, demonstrate different avenues of host-guest chemistry, and identify future directions that exploit the unique combination of properties present in these materials.
24694253	22	124	theme	CNC	3379:3381	arg1	templating					3383:3392	CNC templating	3379:3392	CNC templating	3379:3392	The examples covered in this Account demonstrate that there is a rich diversity of composite materials accessible using CNC templating.
24694253	13	125	dep	leaves	1946:1951	arg1	ordered					1960:1966	ordered	1960:1966	ordered	1960:1966	For example, heating the silica/CNC composites under an inert atmosphere followed by removal of the silica leaves highly ordered, mesoporous carbon films that can be used as supercapacitor electrodes.
24694253	4	126	contain	have	462:465	arg2	property					496:503	an additional, less explored property	467:503	an additional, less explored property	467:503	But CNCs have an additional, less explored property: they organize into a chiral nematic (historically referred to as cholesteric) liquid crystal in water.
24694253	4	126	contain	have	462:465	arg1	CNCs					457:460	CNCs	457:460	CNCs	457:460	But CNCs have an additional, less explored property: they organize into a chiral nematic (historically referred to as cholesteric) liquid crystal in water.
24694253	0	127	theme	mesoporous	34:43	arg1	materials					45:53	chiral nematic mesoporous materials	19:53	chiral nematic mesoporous materials	19:53	The development of chiral nematic mesoporous materials.
24694253	16	128	theme	spacer	2388:2393	arg1	variation					2363:2371	variation	2363:2371	variation of the organic spacer	2363:2393	These materials are flexible and offer the ability to tune the chemical and mechanical properties through variation of the organic spacer.
24694253	17	129	theme	nematic	2403:2409	arg1	hosts					2524:2528	interesting hosts	2512:2528	interesting hosts for nanomaterials	2512:2546	Chiral nematic mesoporous silica and organosilica materials, obtainable as centimeter-scale freestanding films, are interesting hosts for nanomaterials.
24694253	17	129	theme	nematic	2403:2409	arg1	mesoporous					2411:2420	Chiral nematic mesoporous silica and organosilica materials	2396:2454	Chiral nematic mesoporous silica and organosilica materials	2396:2454	Chiral nematic mesoporous silica and organosilica materials, obtainable as centimeter-scale freestanding films, are interesting hosts for nanomaterials.
24694253	20	130	theme	film	2920:2923	arg1	structure					2925:2933	film structure	2920:2933	film structure	2920:2933	The porosity, film structure, and optical properties of these materials could enable their use in sensors.
24694253	13	131	theme	inert	1895:1899	arg1	atmosphere					1901:1910	an inert atmosphere	1892:1910	an inert atmosphere followed by removal of the silica	1892:1944	For example, heating the silica/CNC composites under an inert atmosphere followed by removal of the silica leaves highly ordered, mesoporous carbon films that can be used as supercapacitor electrodes.
24694253	19	132	theme	conjugated	2800:2809	arg1	polymers					2811:2818	Fluorescent conjugated polymers	2788:2818	Fluorescent conjugated polymers	2788:2818	Fluorescent conjugated polymers show induced circular dichroism spectra when encapsulated in the chiral nematic host.
24694253	20	133	theme	materials	2968:2976	arg1	properties					2948:2957	optical properties	2940:2957	optical properties	2940:2957	The porosity, film structure, and optical properties of these materials could enable their use in sensors.
24694253	20	133	theme	materials	2968:2976	arg1	porosity					2910:2917	The porosity	2906:2917	The porosity	2906:2917	The porosity, film structure, and optical properties of these materials could enable their use in sensors.
24694253	20	133	theme	materials	2968:2976	arg1	structure					2925:2933	film structure	2920:2933	film structure	2920:2933	The porosity, film structure, and optical properties of these materials could enable their use in sensors.
24694253	4	134	theme	chiral	527:532	arg1	crystal					591:597	a chiral nematic (historically referred to as cholesteric) liquid crystal	525:597	a chiral nematic (historically referred to as cholesteric) liquid crystal in water	525:606	But CNCs have an additional, less explored property: they organize into a chiral nematic (historically referred to as cholesteric) liquid crystal in water.
24694253	5	135	with	stack	835:839	arg1	pitch					863:867	a characteristic pitch	846:867	a characteristic pitch (repeating distance)	846:888	When dried into a thin solid film, the CNCs retain the helicoidal chiral nematic order and assemble into a layered structure where the CNCs have aligned orientation within each layer, and their orientation rotates through the stack with a characteristic pitch (repeating distance).
24694253	5	135	with	stack	835:839	arg1	distance					880:887	repeating distance	870:887	repeating distance	870:887	When dried into a thin solid film, the CNCs retain the helicoidal chiral nematic order and assemble into a layered structure where the CNCs have aligned orientation within each layer, and their orientation rotates through the stack with a characteristic pitch (repeating distance).
24694253	12	136	theme	nematic	1747:1753	arg1	order					1755:1759	the chiral nematic order	1736:1759	the chiral nematic order	1736:1759	Taking advantage of the chiral nematic order and nanoscale of the CNC templates, new functional materials can be prepared.
24694253	9	137	theme	nematic	1526:1532	arg1	structure					1534:1542	the chiral nematic structure	1515:1542	the chiral nematic structure	1515:1542	Importantly, the silica is brilliantly iridescent because the pores in its interior replicate the chiral nematic structure.
24694253	13	138	theme	silica/CNC	1864:1873	arg1	composites					1875:1884	the silica/CNC composites	1860:1884	the silica/CNC composites	1860:1884	For example, heating the silica/CNC composites under an inert atmosphere followed by removal of the silica leaves highly ordered, mesoporous carbon films that can be used as supercapacitor electrodes.
24694253	21	139	theme	unique	3198:3203	arg1	combination					3205:3215	the unique combination	3194:3215	the unique combination of properties present in these materials	3194:3256	We describe the development of chiral nematic mesoporous silica and organosilica, demonstrate different avenues of host-guest chemistry, and identify future directions that exploit the unique combination of properties present in these materials.
24694253	8	140	from	Calcination	1265:1275	arg1	air					1296:1298	air	1296:1298	air	1296:1298	Calcination of the material in air destroys the cellulose template, leaving a high surface area mesoporous silica film that has pore diameters of ~3-10 nm.
24694253	15	141	theme	organosilica	2224:2235	arg1	films					2237:2241	highly porous, iridescent organosilica films	2198:2241	highly porous, iridescent organosilica films	2198:2241	After removal of the cellulose by acid-catalyzed hydrolysis, highly porous, iridescent organosilica films are obtained.
24694253	20	142	theme	optical	2940:2946	arg1	properties					2948:2957	optical properties	2940:2957	optical properties	2940:2957	The porosity, film structure, and optical properties of these materials could enable their use in sensors.
24694253	8	143	contain	has	1389:1391	arg2	diameters					1398:1406	pore diameters	1393:1406	pore diameters of ~3-10 nm	1393:1418	Calcination of the material in air destroys the cellulose template, leaving a high surface area mesoporous silica film that has pore diameters of ~3-10 nm.
24694253	8	143	contain	has	1389:1391	arg1	film					1379:1382	a high surface area mesoporous silica film	1341:1382	a high surface area mesoporous silica film that has pore diameters of ~3-10 nm	1341:1418	Calcination of the material in air destroys the cellulose template, leaving a high surface area mesoporous silica film that has pore diameters of ~3-10 nm.
24694253	14	144	theme	assorted	2103:2110	arg1	precursors					2125:2134	assorted organosilica precursors	2103:2134	assorted organosilica precursors	2103:2134	The composition of the mesoporous films can be varied by using assorted organosilica precursors.
24694253	8	145	theme	high	1343:1346	arg1	film					1379:1382	a high surface area mesoporous silica film	1341:1382	a high surface area mesoporous silica film that has pore diameters of ~3-10 nm	1341:1418	Calcination of the material in air destroys the cellulose template, leaving a high surface area mesoporous silica film that has pore diameters of ~3-10 nm.
24694253	16	146	theme	chemical	2320:2327	arg1	properties					2344:2353	the chemical and mechanical properties	2316:2353	the chemical and mechanical properties	2316:2353	These materials are flexible and offer the ability to tune the chemical and mechanical properties through variation of the organic spacer.
28156029	2	0	theme	cross	395:399	arg1	complexation					401:412	cross complexation	395:412	cross complexation with the cations	395:429	The process was based on the assumption that particular arrangements of the linear CS chains in aqueous solution, induced so as to accommodate cross complexation with the cations, would be embodied into a tridimensional matrix created through an organoalkoxysilane sol-gel scheme.
28156029	4	1	theme	Pb	820:821	arg1	ions					827:830	the Pb(II) ions	816:830	the Pb(II) ions	816:830	Inversely, for Pb(II), the materials obtained exhibited a "memory" effect for the Pb(II) ions, expressed in the observation of stronger (13%-44%) binding as compared to the nonimprinted counterparts, and increased selectivity (1.5-2 folds) against Cd(II).
28156029	2	2	theme	tridimensional	457:470	arg1	matrix					472:477	a tridimensional matrix	455:477	a tridimensional matrix created through an organoalkoxysilane sol-gel scheme	455:530	The process was based on the assumption that particular arrangements of the linear CS chains in aqueous solution, induced so as to accommodate cross complexation with the cations, would be embodied into a tridimensional matrix created through an organoalkoxysilane sol-gel scheme.
28156029	2	3	theme	sol-gel	517:523	arg1	scheme					525:530	an organoalkoxysilane sol-gel scheme	495:530	an organoalkoxysilane sol-gel scheme	495:530	The process was based on the assumption that particular arrangements of the linear CS chains in aqueous solution, induced so as to accommodate cross complexation with the cations, would be embodied into a tridimensional matrix created through an organoalkoxysilane sol-gel scheme.
28156029	7	4	theme	certain	1515:1521	arg1	cation					1523:1528	a certain cation	1513:1528	a certain cation	1513:1528	The augmented selectivity provided by the cation-imprinting process may be advantageous in areas such as analytical separation, remediation, purification, sensing, and others, particularly in those cases where a certain cation is of special interest within a mixture of them.
28156029	6	5	theme	units	1192:1196	arg1	sulfate					1151:1157	sulfate	1151:1157	sulfate	1151:1157	However, it was not possible to identify, among a set of their properties (carboxylate and sulfate abundance, percent of disulfated units, 4S/6S ratio, and molecular weight), any that correlated directly with the observed imprinting features.
28156029	6	5	theme	units	1192:1196	arg1	weight					1226:1231	molecular weight	1216:1231	molecular weight	1216:1231	However, it was not possible to identify, among a set of their properties (carboxylate and sulfate abundance, percent of disulfated units, 4S/6S ratio, and molecular weight), any that correlated directly with the observed imprinting features.
28156029	6	5	theme	units	1192:1196	arg1	properties					1123:1132	their properties	1117:1132	their properties (carboxylate and sulfate abundance, percent of disulfated units, 4S/6S ratio, and molecular weight)	1117:1232	However, it was not possible to identify, among a set of their properties (carboxylate and sulfate abundance, percent of disulfated units, 4S/6S ratio, and molecular weight), any that correlated directly with the observed imprinting features.
28156029	6	5	theme	units	1192:1196	arg1	percent					1170:1176	percent	1170:1176	percent of disulfated units	1170:1196	However, it was not possible to identify, among a set of their properties (carboxylate and sulfate abundance, percent of disulfated units, 4S/6S ratio, and molecular weight), any that correlated directly with the observed imprinting features.
28156029	6	5	theme	units	1192:1196	arg1	ratio					1205:1209	4S/6S ratio	1199:1209	4S/6S ratio	1199:1209	However, it was not possible to identify, among a set of their properties (carboxylate and sulfate abundance, percent of disulfated units, 4S/6S ratio, and molecular weight), any that correlated directly with the observed imprinting features.
28156029	6	5	theme	units	1192:1196	arg1	carboxylate					1135:1145	carboxylate	1135:1145	carboxylate	1135:1145	However, it was not possible to identify, among a set of their properties (carboxylate and sulfate abundance, percent of disulfated units, 4S/6S ratio, and molecular weight), any that correlated directly with the observed imprinting features.
28156029	7	6	theme	cation-imprinting	1345:1361	arg1	process					1363:1369	the cation-imprinting process	1341:1369	the cation-imprinting process	1341:1369	The augmented selectivity provided by the cation-imprinting process may be advantageous in areas such as analytical separation, remediation, purification, sensing, and others, particularly in those cases where a certain cation is of special interest within a mixture of them.
28156029	2	7	from	solution	356:363	arg1	arrangements					308:319	particular arrangements	297:319	particular arrangements	297:319	The process was based on the assumption that particular arrangements of the linear CS chains in aqueous solution, induced so as to accommodate cross complexation with the cations, would be embodied into a tridimensional matrix created through an organoalkoxysilane sol-gel scheme.
28156029	2	8	theme	organoalkoxysilane	498:515	arg1	scheme					525:530	an organoalkoxysilane sol-gel scheme	495:530	an organoalkoxysilane sol-gel scheme	495:530	The process was based on the assumption that particular arrangements of the linear CS chains in aqueous solution, induced so as to accommodate cross complexation with the cations, would be embodied into a tridimensional matrix created through an organoalkoxysilane sol-gel scheme.
28156029	4	9	dep	stronger	865:872	arg1	%					881:881	13%-44%	875:881	13%-44%	875:881	Inversely, for Pb(II), the materials obtained exhibited a "memory" effect for the Pb(II) ions, expressed in the observation of stronger (13%-44%) binding as compared to the nonimprinted counterparts, and increased selectivity (1.5-2 folds) against Cd(II).
28156029	4	10	theme	stronger	865:872	arg1	binding					884:890	stronger (13%-44%) binding	865:890	stronger (13%-44%) binding as compared to the nonimprinted counterparts	865:935	Inversely, for Pb(II), the materials obtained exhibited a "memory" effect for the Pb(II) ions, expressed in the observation of stronger (13%-44%) binding as compared to the nonimprinted counterparts, and increased selectivity (1.5-2 folds) against Cd(II).
28156029	0	11	theme	chondroitin	80:90	arg1	sulfate					92:98	chondroitin sulfate	80:98	chondroitin sulfate	80:98	Cationic imprinting of Pb(II) within composite networks based on bovine or fish chondroitin sulfate.
28156029	2	12	with	complexation	401:412	arg1	cations					423:429	the cations	419:429	the cations	419:429	The process was based on the assumption that particular arrangements of the linear CS chains in aqueous solution, induced so as to accommodate cross complexation with the cations, would be embodied into a tridimensional matrix created through an organoalkoxysilane sol-gel scheme.
28156029	1	13	theme	/silica	136:142	arg1	composites					144:153	Imprinting chondroitin sulfate (CS)/silica composites	101:153	Imprinting chondroitin sulfate (CS)/silica composites with Pb(II) and Cu(II) cations	101:184	Imprinting chondroitin sulfate (CS)/silica composites with Pb(II) and Cu(II) cations was explored with CS of bovine and different fish species origin.
28156029	4	14	theme	memory	797:802	arg1	effect					805:810	a "memory" effect	794:810	a "memory" effect	794:810	Inversely, for Pb(II), the materials obtained exhibited a "memory" effect for the Pb(II) ions, expressed in the observation of stronger (13%-44%) binding as compared to the nonimprinted counterparts, and increased selectivity (1.5-2 folds) against Cd(II).
28156029	0	15	dep	bovine	65:70	arg1	sulfate					92:98	chondroitin sulfate	80:98	chondroitin sulfate	80:98	Cationic imprinting of Pb(II) within composite networks based on bovine or fish chondroitin sulfate.
28156029	3	16	from	presence	537:544	arg1	synthesis					563:571	the synthesis	559:571	the synthesis of the composites	559:589	The presence of Cu(II) in the synthesis of the composites did not result in the production of significantly stronger Cu(II)-oriented binding arrangements, and therefore, the imprinting was not successful.
28156029	4	17	theme	"	803:803	arg1	effect					805:810	a "memory" effect	794:810	a "memory" effect	794:810	Inversely, for Pb(II), the materials obtained exhibited a "memory" effect for the Pb(II) ions, expressed in the observation of stronger (13%-44%) binding as compared to the nonimprinted counterparts, and increased selectivity (1.5-2 folds) against Cd(II).
28156029	6	18	dep	properties	1123:1132	arg1	sulfate					1151:1157	sulfate	1151:1157	sulfate	1151:1157	However, it was not possible to identify, among a set of their properties (carboxylate and sulfate abundance, percent of disulfated units, 4S/6S ratio, and molecular weight), any that correlated directly with the observed imprinting features.
28156029	6	18	dep	properties	1123:1132	arg1	weight					1226:1231	molecular weight	1216:1231	molecular weight	1216:1231	However, it was not possible to identify, among a set of their properties (carboxylate and sulfate abundance, percent of disulfated units, 4S/6S ratio, and molecular weight), any that correlated directly with the observed imprinting features.
28156029	6	18	dep	properties	1123:1132	arg1	properties					1123:1132	their properties	1117:1132	their properties (carboxylate and sulfate abundance, percent of disulfated units, 4S/6S ratio, and molecular weight)	1117:1232	However, it was not possible to identify, among a set of their properties (carboxylate and sulfate abundance, percent of disulfated units, 4S/6S ratio, and molecular weight), any that correlated directly with the observed imprinting features.
28156029	6	18	dep	properties	1123:1132	arg1	percent					1170:1176	percent	1170:1176	percent of disulfated units	1170:1196	However, it was not possible to identify, among a set of their properties (carboxylate and sulfate abundance, percent of disulfated units, 4S/6S ratio, and molecular weight), any that correlated directly with the observed imprinting features.
28156029	6	18	dep	properties	1123:1132	arg1	ratio					1205:1209	4S/6S ratio	1199:1209	4S/6S ratio	1199:1209	However, it was not possible to identify, among a set of their properties (carboxylate and sulfate abundance, percent of disulfated units, 4S/6S ratio, and molecular weight), any that correlated directly with the observed imprinting features.
28156029	6	18	dep	properties	1123:1132	arg1	carboxylate					1135:1145	carboxylate	1135:1145	carboxylate	1135:1145	However, it was not possible to identify, among a set of their properties (carboxylate and sulfate abundance, percent of disulfated units, 4S/6S ratio, and molecular weight), any that correlated directly with the observed imprinting features.
28156029	4	19	theme	nonimprinted	911:922	arg1	counterparts					924:935	the nonimprinted counterparts	907:935	the nonimprinted counterparts	907:935	Inversely, for Pb(II), the materials obtained exhibited a "memory" effect for the Pb(II) ions, expressed in the observation of stronger (13%-44%) binding as compared to the nonimprinted counterparts, and increased selectivity (1.5-2 folds) against Cd(II).
28156029	2	20	theme	particular	297:306	arg1	arrangements					308:319	particular arrangements	297:319	particular arrangements	297:319	The process was based on the assumption that particular arrangements of the linear CS chains in aqueous solution, induced so as to accommodate cross complexation with the cations, would be embodied into a tridimensional matrix created through an organoalkoxysilane sol-gel scheme.
28156029	0	21	theme	Cationic	0:7	arg1	imprinting					9:18	Cationic imprinting	0:18	Cationic imprinting of Pb(II) within composite networks based on bovine or fish chondroitin sulfate.	0:99	Cationic imprinting of Pb(II) within composite networks based on bovine or fish chondroitin sulfate.
28156029	3	22	theme	stronger	641:648	arg1	arrangements					674:685	significantly stronger Cu(II)-oriented binding arrangements	627:685	significantly stronger Cu(II)-oriented binding arrangements	627:685	The presence of Cu(II) in the synthesis of the composites did not result in the production of significantly stronger Cu(II)-oriented binding arrangements, and therefore, the imprinting was not successful.
28156029	7	23	from	advantageous	1378:1389	arg1	others					1471:1476	others	1471:1476	others	1471:1476	The augmented selectivity provided by the cation-imprinting process may be advantageous in areas such as analytical separation, remediation, purification, sensing, and others, particularly in those cases where a certain cation is of special interest within a mixture of them.
28156029	7	23	from	advantageous	1378:1389	arg1	areas					1394:1398	areas	1394:1398	areas	1394:1398	The augmented selectivity provided by the cation-imprinting process may be advantageous in areas such as analytical separation, remediation, purification, sensing, and others, particularly in those cases where a certain cation is of special interest within a mixture of them.
28156029	7	23	from	advantageous	1378:1389	arg1	purification					1444:1455	purification	1444:1455	purification	1444:1455	The augmented selectivity provided by the cation-imprinting process may be advantageous in areas such as analytical separation, remediation, purification, sensing, and others, particularly in those cases where a certain cation is of special interest within a mixture of them.
28156029	7	23	from	advantageous	1378:1389	arg1	sensing					1458:1464	sensing	1458:1464	sensing	1458:1464	The augmented selectivity provided by the cation-imprinting process may be advantageous in areas such as analytical separation, remediation, purification, sensing, and others, particularly in those cases where a certain cation is of special interest within a mixture of them.
28156029	7	23	from	advantageous	1378:1389	arg1	separation					1419:1428	analytical separation	1408:1428	analytical separation	1408:1428	The augmented selectivity provided by the cation-imprinting process may be advantageous in areas such as analytical separation, remediation, purification, sensing, and others, particularly in those cases where a certain cation is of special interest within a mixture of them.
28156029	7	23	from	advantageous	1378:1389	arg1	remediation					1431:1441	remediation	1431:1441	remediation	1431:1441	The augmented selectivity provided by the cation-imprinting process may be advantageous in areas such as analytical separation, remediation, purification, sensing, and others, particularly in those cases where a certain cation is of special interest within a mixture of them.
28156029	3	24	theme	-oriented	656:664	arg1	arrangements					674:685	significantly stronger Cu(II)-oriented binding arrangements	627:685	significantly stronger Cu(II)-oriented binding arrangements	627:685	The presence of Cu(II) in the synthesis of the composites did not result in the production of significantly stronger Cu(II)-oriented binding arrangements, and therefore, the imprinting was not successful.
28156029	0	25	theme	Pb	23:24	arg1	imprinting					9:18	Cationic imprinting	0:18	Cationic imprinting of Pb(II) within composite networks based on bovine or fish chondroitin sulfate.	0:99	Cationic imprinting of Pb(II) within composite networks based on bovine or fish chondroitin sulfate.
28156029	3	26	theme	Cu	650:651	arg1	arrangements					674:685	significantly stronger Cu(II)-oriented binding arrangements	627:685	significantly stronger Cu(II)-oriented binding arrangements	627:685	The presence of Cu(II) in the synthesis of the composites did not result in the production of significantly stronger Cu(II)-oriented binding arrangements, and therefore, the imprinting was not successful.
28156029	6	27	dep	carboxylate	1135:1145	arg1	abundance					1159:1167	abundance	1159:1167	abundance	1159:1167	However, it was not possible to identify, among a set of their properties (carboxylate and sulfate abundance, percent of disulfated units, 4S/6S ratio, and molecular weight), any that correlated directly with the observed imprinting features.
28156029	2	28	theme	chains	338:343	arg1	arrangements					308:319	particular arrangements	297:319	particular arrangements	297:319	The process was based on the assumption that particular arrangements of the linear CS chains in aqueous solution, induced so as to accommodate cross complexation with the cations, would be embodied into a tridimensional matrix created through an organoalkoxysilane sol-gel scheme.
28156029	6	29	theme	imprinting	1282:1291	arg1	features					1293:1300	the observed imprinting features	1269:1300	the observed imprinting features	1269:1300	However, it was not possible to identify, among a set of their properties (carboxylate and sulfate abundance, percent of disulfated units, 4S/6S ratio, and molecular weight), any that correlated directly with the observed imprinting features.
28156029	1	30	theme	bovine	210:215	arg1	species					236:242	bovine and different fish species	210:242	species	236:242	Imprinting chondroitin sulfate (CS)/silica composites with Pb(II) and Cu(II) cations was explored with CS of bovine and different fish species origin.
28156029	4	31	dep	1.5-2	965:969	arg1	folds					971:975	folds	971:975	folds	971:975	Inversely, for Pb(II), the materials obtained exhibited a "memory" effect for the Pb(II) ions, expressed in the observation of stronger (13%-44%) binding as compared to the nonimprinted counterparts, and increased selectivity (1.5-2 folds) against Cd(II).
28156029	2	32	theme	CS	335:336	arg1	chains					338:343	the linear CS chains	324:343	the linear CS chains in aqueous solution	324:363	The process was based on the assumption that particular arrangements of the linear CS chains in aqueous solution, induced so as to accommodate cross complexation with the cations, would be embodied into a tridimensional matrix created through an organoalkoxysilane sol-gel scheme.
28156029	2	33	theme	linear	328:333	arg1	chains					338:343	the linear CS chains	324:343	the linear CS chains in aqueous solution	324:363	The process was based on the assumption that particular arrangements of the linear CS chains in aqueous solution, induced so as to accommodate cross complexation with the cations, would be embodied into a tridimensional matrix created through an organoalkoxysilane sol-gel scheme.
28156029	6	34	theme	molecular	1216:1224	arg1	weight					1226:1231	molecular weight	1216:1231	molecular weight	1216:1231	However, it was not possible to identify, among a set of their properties (carboxylate and sulfate abundance, percent of disulfated units, 4S/6S ratio, and molecular weight), any that correlated directly with the observed imprinting features.
28156029	6	34	theme	molecular	1216:1224	arg1	properties					1123:1132	their properties	1117:1132	their properties (carboxylate and sulfate abundance, percent of disulfated units, 4S/6S ratio, and molecular weight)	1117:1232	However, it was not possible to identify, among a set of their properties (carboxylate and sulfate abundance, percent of disulfated units, 4S/6S ratio, and molecular weight), any that correlated directly with the observed imprinting features.
28156029	1	35	theme	different	221:229	arg1	species					236:242	bovine and different fish species	210:242	species	236:242	Imprinting chondroitin sulfate (CS)/silica composites with Pb(II) and Cu(II) cations was explored with CS of bovine and different fish species origin.
28156029	3	36	theme	Cu	549:550	arg1	presence					537:544	The presence	533:544	The presence of Cu(II) in the synthesis of the composites	533:589	The presence of Cu(II) in the synthesis of the composites did not result in the production of significantly stronger Cu(II)-oriented binding arrangements, and therefore, the imprinting was not successful.
28156029	1	37	dep	Pb	160:161	arg1	cations					178:184	cations	178:184	cations	178:184	Imprinting chondroitin sulfate (CS)/silica composites with Pb(II) and Cu(II) cations was explored with CS of bovine and different fish species origin.
28156029	7	38	from	areas	1394:1398	arg1	cases					1501:1505	those cases	1495:1505	those cases where a certain cation is of special interest within a mixture of them	1495:1576	The augmented selectivity provided by the cation-imprinting process may be advantageous in areas such as analytical separation, remediation, purification, sensing, and others, particularly in those cases where a certain cation is of special interest within a mixture of them.
28156029	7	38	from	areas	1394:1398	arg1	selectivity					1317:1327	The augmented selectivity	1303:1327	The augmented selectivity provided by the cation-imprinting process	1303:1369	The augmented selectivity provided by the cation-imprinting process may be advantageous in areas such as analytical separation, remediation, purification, sensing, and others, particularly in those cases where a certain cation is of special interest within a mixture of them.
28156029	7	38	from	areas	1394:1398	arg1	advantageous					1378:1389	advantageous	1378:1389	advantageous	1378:1389	The augmented selectivity provided by the cation-imprinting process may be advantageous in areas such as analytical separation, remediation, purification, sensing, and others, particularly in those cases where a certain cation is of special interest within a mixture of them.
28156029	1	39	theme	fish	231:234	arg1	species					236:242	bovine and different fish species	210:242	species	236:242	Imprinting chondroitin sulfate (CS)/silica composites with Pb(II) and Cu(II) cations was explored with CS of bovine and different fish species origin.
28156029	6	40	theme	observed	1273:1280	arg1	features					1293:1300	the observed imprinting features	1269:1300	the observed imprinting features	1269:1300	However, it was not possible to identify, among a set of their properties (carboxylate and sulfate abundance, percent of disulfated units, 4S/6S ratio, and molecular weight), any that correlated directly with the observed imprinting features.
28156029	2	41	theme	aqueous	348:354	arg1	solution					356:363	aqueous solution	348:363	aqueous solution	348:363	The process was based on the assumption that particular arrangements of the linear CS chains in aqueous solution, induced so as to accommodate cross complexation with the cations, would be embodied into a tridimensional matrix created through an organoalkoxysilane sol-gel scheme.
28156029	1	42	theme	species	236:242	arg1	origin					244:249	bovine and different fish species origin	210:249	bovine and different fish species origin	210:249	Imprinting chondroitin sulfate (CS)/silica composites with Pb(II) and Cu(II) cations was explored with CS of bovine and different fish species origin.
28156029	4	43	dep	binding	884:890	arg1	compared					895:902	compared	895:902	compared to the nonimprinted counterparts	895:935	Inversely, for Pb(II), the materials obtained exhibited a "memory" effect for the Pb(II) ions, expressed in the observation of stronger (13%-44%) binding as compared to the nonimprinted counterparts, and increased selectivity (1.5-2 folds) against Cd(II).
28156029	4	44	theme	%	877:877	arg1	%					881:881	13%-44%	875:881	13%-44%	875:881	Inversely, for Pb(II), the materials obtained exhibited a "memory" effect for the Pb(II) ions, expressed in the observation of stronger (13%-44%) binding as compared to the nonimprinted counterparts, and increased selectivity (1.5-2 folds) against Cd(II).
28156029	1	45	theme	origin	244:249	arg1	CS					204:205	CS	204:205	CS of bovine and different fish species origin	204:249	Imprinting chondroitin sulfate (CS)/silica composites with Pb(II) and Cu(II) cations was explored with CS of bovine and different fish species origin.
28156029	7	46	theme	them	1573:1576	arg1	mixture					1562:1568	a mixture	1560:1568	a mixture of them	1560:1576	The augmented selectivity provided by the cation-imprinting process may be advantageous in areas such as analytical separation, remediation, purification, sensing, and others, particularly in those cases where a certain cation is of special interest within a mixture of them.
28156029	2	47	from	arrangements	308:319	arg1	solution					356:363	aqueous solution	348:363	aqueous solution	348:363	The process was based on the assumption that particular arrangements of the linear CS chains in aqueous solution, induced so as to accommodate cross complexation with the cations, would be embodied into a tridimensional matrix created through an organoalkoxysilane sol-gel scheme.
28156029	1	48	theme	Imprinting	101:110	arg1	composites					144:153	Imprinting chondroitin sulfate (CS)/silica composites	101:153	Imprinting chondroitin sulfate (CS)/silica composites with Pb(II) and Cu(II) cations	101:184	Imprinting chondroitin sulfate (CS)/silica composites with Pb(II) and Cu(II) cations was explored with CS of bovine and different fish species origin.
28156029	3	49	attach	presence	537:544	arg2	Cu					549:550	Cu(II)	549:554	Cu(II)	549:554	The presence of Cu(II) in the synthesis of the composites did not result in the production of significantly stronger Cu(II)-oriented binding arrangements, and therefore, the imprinting was not successful.
28156029	3	49	attach	presence	537:544	arg1	synthesis					563:571	the synthesis	559:571	the synthesis of the composites	559:589	The presence of Cu(II) in the synthesis of the composites did not result in the production of significantly stronger Cu(II)-oriented binding arrangements, and therefore, the imprinting was not successful.
28156029	7	50	theme	augmented	1307:1315	arg1	advantageous					1378:1389	advantageous	1378:1389	advantageous	1378:1389	The augmented selectivity provided by the cation-imprinting process may be advantageous in areas such as analytical separation, remediation, purification, sensing, and others, particularly in those cases where a certain cation is of special interest within a mixture of them.
28156029	7	50	theme	augmented	1307:1315	arg1	selectivity					1317:1327	The augmented selectivity	1303:1327	The augmented selectivity provided by the cation-imprinting process	1303:1369	The augmented selectivity provided by the cation-imprinting process may be advantageous in areas such as analytical separation, remediation, purification, sensing, and others, particularly in those cases where a certain cation is of special interest within a mixture of them.
28156029	1	51	with	composites	144:153	arg1	II					163:164	II	163:164	II	163:164	Imprinting chondroitin sulfate (CS)/silica composites with Pb(II) and Cu(II) cations was explored with CS of bovine and different fish species origin.
28156029	1	51	with	composites	144:153	arg1	Cu					171:172	Cu(II)	171:176	Cu(II)	171:176	Imprinting chondroitin sulfate (CS)/silica composites with Pb(II) and Cu(II) cations was explored with CS of bovine and different fish species origin.
28156029	1	51	with	composites	144:153	arg1	Pb					160:161	Pb	160:161	Pb(II)	160:165	Imprinting chondroitin sulfate (CS)/silica composites with Pb(II) and Cu(II) cations was explored with CS of bovine and different fish species origin.
28156029	7	52	theme	special	1536:1542	arg1	interest					1544:1551	special interest	1536:1551	special interest	1536:1551	The augmented selectivity provided by the cation-imprinting process may be advantageous in areas such as analytical separation, remediation, purification, sensing, and others, particularly in those cases where a certain cation is of special interest within a mixture of them.
28156029	1	53	theme	chondroitin	112:122	arg1	CS					133:134	CS	133:134	CS	133:134	Imprinting chondroitin sulfate (CS)/silica composites with Pb(II) and Cu(II) cations was explored with CS of bovine and different fish species origin.
28156029	1	53	theme	chondroitin	112:122	arg1	sulfate					124:130	chondroitin sulfate	112:130	Imprinting chondroitin sulfate (CS)/silica composites with Pb(II) and Cu(II) cations	101:184	Imprinting chondroitin sulfate (CS)/silica composites with Pb(II) and Cu(II) cations was explored with CS of bovine and different fish species origin.
28156029	5	54	theme	CS	1049:1050	arg1	source					1052:1057	the CS source	1045:1057	the CS source	1045:1057	The imprinting features observed were dependent on the CS source.
28156029	7	55	theme	analytical	1408:1417	arg1	separation					1419:1428	analytical separation	1408:1428	analytical separation	1408:1428	The augmented selectivity provided by the cation-imprinting process may be advantageous in areas such as analytical separation, remediation, purification, sensing, and others, particularly in those cases where a certain cation is of special interest within a mixture of them.
28156029	6	56	theme	properties	1123:1132	arg1	sulfate					1151:1157	sulfate	1151:1157	sulfate	1151:1157	However, it was not possible to identify, among a set of their properties (carboxylate and sulfate abundance, percent of disulfated units, 4S/6S ratio, and molecular weight), any that correlated directly with the observed imprinting features.
28156029	6	56	theme	properties	1123:1132	arg1	set					1110:1112	a set	1108:1112	a set of their properties (carboxylate and sulfate abundance, percent of disulfated units, 4S/6S ratio, and molecular weight)	1108:1232	However, it was not possible to identify, among a set of their properties (carboxylate and sulfate abundance, percent of disulfated units, 4S/6S ratio, and molecular weight), any that correlated directly with the observed imprinting features.
28156029	6	56	theme	properties	1123:1132	arg1	weight					1226:1231	molecular weight	1216:1231	molecular weight	1216:1231	However, it was not possible to identify, among a set of their properties (carboxylate and sulfate abundance, percent of disulfated units, 4S/6S ratio, and molecular weight), any that correlated directly with the observed imprinting features.
28156029	6	56	theme	properties	1123:1132	arg1	properties					1123:1132	their properties	1117:1132	their properties (carboxylate and sulfate abundance, percent of disulfated units, 4S/6S ratio, and molecular weight)	1117:1232	However, it was not possible to identify, among a set of their properties (carboxylate and sulfate abundance, percent of disulfated units, 4S/6S ratio, and molecular weight), any that correlated directly with the observed imprinting features.
28156029	6	56	theme	properties	1123:1132	arg1	percent					1170:1176	percent	1170:1176	percent of disulfated units	1170:1196	However, it was not possible to identify, among a set of their properties (carboxylate and sulfate abundance, percent of disulfated units, 4S/6S ratio, and molecular weight), any that correlated directly with the observed imprinting features.
28156029	6	56	theme	properties	1123:1132	arg1	ratio					1205:1209	4S/6S ratio	1199:1209	4S/6S ratio	1199:1209	However, it was not possible to identify, among a set of their properties (carboxylate and sulfate abundance, percent of disulfated units, 4S/6S ratio, and molecular weight), any that correlated directly with the observed imprinting features.
28156029	6	56	theme	properties	1123:1132	arg1	carboxylate					1135:1145	carboxylate	1135:1145	carboxylate	1135:1145	However, it was not possible to identify, among a set of their properties (carboxylate and sulfate abundance, percent of disulfated units, 4S/6S ratio, and molecular weight), any that correlated directly with the observed imprinting features.
28156029	6	57	theme	disulfated	1181:1190	arg1	units					1192:1196	disulfated units	1181:1196	disulfated units	1181:1196	However, it was not possible to identify, among a set of their properties (carboxylate and sulfate abundance, percent of disulfated units, 4S/6S ratio, and molecular weight), any that correlated directly with the observed imprinting features.
28156029	3	58	theme	composites	580:589	arg1	synthesis					563:571	the synthesis	559:571	the synthesis of the composites	559:589	The presence of Cu(II) in the synthesis of the composites did not result in the production of significantly stronger Cu(II)-oriented binding arrangements, and therefore, the imprinting was not successful.
28156029	6	59	theme	4S/6S	1199:1203	arg1	ratio					1205:1209	4S/6S ratio	1199:1209	4S/6S ratio	1199:1209	However, it was not possible to identify, among a set of their properties (carboxylate and sulfate abundance, percent of disulfated units, 4S/6S ratio, and molecular weight), any that correlated directly with the observed imprinting features.
28156029	6	59	theme	4S/6S	1199:1203	arg1	properties					1123:1132	their properties	1117:1132	their properties (carboxylate and sulfate abundance, percent of disulfated units, 4S/6S ratio, and molecular weight)	1117:1232	However, it was not possible to identify, among a set of their properties (carboxylate and sulfate abundance, percent of disulfated units, 4S/6S ratio, and molecular weight), any that correlated directly with the observed imprinting features.
28156029	1	60	theme	sulfate	124:130	arg1	composites					144:153	Imprinting chondroitin sulfate (CS)/silica composites	101:153	Imprinting chondroitin sulfate (CS)/silica composites with Pb(II) and Cu(II) cations	101:184	Imprinting chondroitin sulfate (CS)/silica composites with Pb(II) and Cu(II) cations was explored with CS of bovine and different fish species origin.
28156029	3	61	theme	binding	666:672	arg1	arrangements					674:685	significantly stronger Cu(II)-oriented binding arrangements	627:685	significantly stronger Cu(II)-oriented binding arrangements	627:685	The presence of Cu(II) in the synthesis of the composites did not result in the production of significantly stronger Cu(II)-oriented binding arrangements, and therefore, the imprinting was not successful.
28156029	0	62	theme	composite	37:45	arg1	networks					47:54	composite networks	37:54	composite networks based on bovine or fish chondroitin sulfate	37:98	Cationic imprinting of Pb(II) within composite networks based on bovine or fish chondroitin sulfate.
28156029	5	63	theme	imprinting	998:1007	arg1	features					1009:1016	The imprinting features	994:1016	The imprinting features observed	994:1025	The imprinting features observed were dependent on the CS source.
28156029	5	63	theme	imprinting	998:1007	arg1	dependent					1032:1040	dependent	1032:1040	dependent	1032:1040	The imprinting features observed were dependent on the CS source.
28156029	2	64	from	chains	338:343	arg1	solution					356:363	aqueous solution	348:363	aqueous solution	348:363	The process was based on the assumption that particular arrangements of the linear CS chains in aqueous solution, induced so as to accommodate cross complexation with the cations, would be embodied into a tridimensional matrix created through an organoalkoxysilane sol-gel scheme.
28156029	4	65	theme	binding	884:890	arg1	observation					850:860	the observation	846:860	the observation of stronger (13%-44%) binding as compared to the nonimprinted counterparts	846:935	Inversely, for Pb(II), the materials obtained exhibited a "memory" effect for the Pb(II) ions, expressed in the observation of stronger (13%-44%) binding as compared to the nonimprinted counterparts, and increased selectivity (1.5-2 folds) against Cd(II).
28156029	3	66	theme	arrangements	674:685	arg1	production					613:622	the production	609:622	the production of significantly stronger Cu(II)-oriented binding arrangements	609:685	The presence of Cu(II) in the synthesis of the composites did not result in the production of significantly stronger Cu(II)-oriented binding arrangements, and therefore, the imprinting was not successful.
28249765	7	0	from	results	771:777	arg1	evident					754:760	evident	754:760	evident	754:760	It is also evident from TGA results that thermal stability of the composites were enhanced with addition of seaweed.
28249765	6	1	theme	plane	736:740	arg1	formation					714:722	formation	714:722	formation of cleavage plane	714:740	Scanning electron micrograph of the tensile fracture showed homogeneous surface with formation of cleavage plane.
28249765	1	2	theme	paper	140:144	arg1	aim					128:130	The aim	124:130	The aim of this paper	124:144	The aim of this paper is to investigate the characteristics of thermoplastic sugar palm starch/agar (TPSA) blend containing Eucheuma cottonii seaweed waste as biofiller.
28249765	6	3	theme	Scanning	629:636	arg1	micrograph					647:656	Scanning electron micrograph	629:656	Scanning electron micrograph of the tensile fracture	629:680	Scanning electron micrograph of the tensile fracture showed homogeneous surface with formation of cleavage plane.
28249765	1	4	theme	blend	231:235	arg1	characteristics					168:182	the characteristics	164:182	the characteristics of thermoplastic sugar palm starch/agar (TPSA) blend containing Eucheuma cottonii seaweed waste as biofiller	164:291	The aim of this paper is to investigate the characteristics of thermoplastic sugar palm starch/agar (TPSA) blend containing Eucheuma cottonii seaweed waste as biofiller.
28249765	8	5	theme	seaweed	964:970	arg1	addition					952:959	addition	952:959	addition of seaweed	952:970	After soil burial for 2 and 4 weeks, the biodegradation of the composites was enhanced with addition of seaweed.
28249765	2	6	theme	hot	342:344	arg1	pressing					346:353	melt-mixing and hot pressing	326:353	melt-mixing and hot pressing	326:353	The composites were prepared by melt-mixing and hot pressing at 140°C for 10min.
28249765	8	7	theme	soil	866:869	arg1	burial					871:876	soil burial	866:876	soil burial for 2 and 4 weeks	866:894	After soil burial for 2 and 4 weeks, the biodegradation of the composites was enhanced with addition of seaweed.
28249765	0	8	theme	sugar	89:93	arg1	composites					112:121	thermoplastic sugar palm starch/agar composites	75:121	thermoplastic sugar palm starch/agar composites	75:121	Effect of seaweed on mechanical, thermal, and biodegradation properties of thermoplastic sugar palm starch/agar composites.
28249765	0	9	from	Effect	0:5	arg1	properties					61:70	mechanical, thermal, and biodegradation properties	21:70	mechanical, thermal, and biodegradation properties of thermoplastic sugar palm starch/agar composites	21:121	Effect of seaweed on mechanical, thermal, and biodegradation properties of thermoplastic sugar palm starch/agar composites.
28249765	9	10	theme	seaweed	1003:1009	arg1	incorporation					986:998	the incorporation	982:998	the incorporation of seaweed into TPSA	982:1019	Overall, the incorporation of seaweed into TPSA enhances the properties of TPSA for short-life product application such as tray, plate, etc.
28249765	9	11	theme	TPSA	1048:1051	arg1	properties					1034:1043	the properties	1030:1043	the properties of TPSA for short-life product application such as tray, plate, etc	1030:1111	Overall, the incorporation of seaweed into TPSA enhances the properties of TPSA for short-life product application such as tray, plate, etc.
28249765	9	11	theme	TPSA	1048:1051	arg1	tray					1096:1099	tray	1096:1099	tray	1096:1099	Overall, the incorporation of seaweed into TPSA enhances the properties of TPSA for short-life product application such as tray, plate, etc.
28249765	1	12	theme	Eucheuma	248:255	arg1	waste					274:278	Eucheuma cottonii seaweed waste	248:278	Eucheuma cottonii seaweed waste	248:278	The aim of this paper is to investigate the characteristics of thermoplastic sugar palm starch/agar (TPSA) blend containing Eucheuma cottonii seaweed waste as biofiller.
28249765	0	13	theme	thermoplastic	75:87	arg1	composites					112:121	thermoplastic sugar palm starch/agar composites	75:121	thermoplastic sugar palm starch/agar composites	75:121	Effect of seaweed on mechanical, thermal, and biodegradation properties of thermoplastic sugar palm starch/agar composites.
28249765	6	14	theme	cleavage	727:734	arg1	plane					736:740	cleavage plane	727:740	cleavage plane	727:740	Scanning electron micrograph of the tensile fracture showed homogeneous surface with formation of cleavage plane.
28249765	1	15	theme	cottonii	257:264	arg1	waste					274:278	Eucheuma cottonii seaweed waste	248:278	Eucheuma cottonii seaweed waste	248:278	The aim of this paper is to investigate the characteristics of thermoplastic sugar palm starch/agar (TPSA) blend containing Eucheuma cottonii seaweed waste as biofiller.
28249765	0	16	theme	starch/agar	100:110	arg1	composites					112:121	thermoplastic sugar palm starch/agar composites	75:121	thermoplastic sugar palm starch/agar composites	75:121	Effect of seaweed on mechanical, thermal, and biodegradation properties of thermoplastic sugar palm starch/agar composites.
28249765	5	17	theme	composites	617:626	arg1	properties					586:595	the tensile, flexural, and impact properties	552:595	the tensile, flexural, and impact properties of the TPSA/seaweed composites	552:626	% has significantly improved the tensile, flexural, and impact properties of the TPSA/seaweed composites.
28249765	5	18	theme	TPSA/seaweed	604:615	arg1	composites					617:626	the TPSA/seaweed composites	600:626	the TPSA/seaweed composites	600:626	% has significantly improved the tensile, flexural, and impact properties of the TPSA/seaweed composites.
28249765	9	19	theme	short-life	1057:1066	arg1	application					1076:1086	short-life product application	1057:1086	short-life product application	1057:1086	Overall, the incorporation of seaweed into TPSA enhances the properties of TPSA for short-life product application such as tray, plate, etc.
28249765	3	20	theme	mechanical	432:441	arg1	properties					471:480	their mechanical, thermal and biodegradation properties	426:480	their mechanical, thermal and biodegradation properties	426:480	The TPSA/seaweed composites were characterized for their mechanical, thermal and biodegradation properties.
28249765	0	21	theme	palm	95:98	arg1	composites					112:121	thermoplastic sugar palm starch/agar composites	75:121	thermoplastic sugar palm starch/agar composites	75:121	Effect of seaweed on mechanical, thermal, and biodegradation properties of thermoplastic sugar palm starch/agar composites.
28249765	5	22	theme	flexural	565:572	arg1	properties					586:595	the tensile, flexural, and impact properties	552:595	the tensile, flexural, and impact properties of the TPSA/seaweed composites	552:626	% has significantly improved the tensile, flexural, and impact properties of the TPSA/seaweed composites.
28249765	7	23	theme	seaweed	851:857	arg1	addition					839:846	addition	839:846	addition of seaweed	839:857	It is also evident from TGA results that thermal stability of the composites were enhanced with addition of seaweed.
28249765	7	24	theme	composites	809:818	arg1	stability					792:800	thermal stability	784:800	thermal stability of the composites	784:818	It is also evident from TGA results that thermal stability of the composites were enhanced with addition of seaweed.
28249765	1	25	theme	seaweed	266:272	arg1	waste					274:278	Eucheuma cottonii seaweed waste	248:278	Eucheuma cottonii seaweed waste	248:278	The aim of this paper is to investigate the characteristics of thermoplastic sugar palm starch/agar (TPSA) blend containing Eucheuma cottonii seaweed waste as biofiller.
28249765	9	26	theme	product	1068:1074	arg1	application					1076:1086	short-life product application	1057:1086	short-life product application	1057:1086	Overall, the incorporation of seaweed into TPSA enhances the properties of TPSA for short-life product application such as tray, plate, etc.
28249765	5	27	theme	impact	579:584	arg1	properties					586:595	the tensile, flexural, and impact properties	552:595	the tensile, flexural, and impact properties of the TPSA/seaweed composites	552:626	% has significantly improved the tensile, flexural, and impact properties of the TPSA/seaweed composites.
28249765	6	28	theme	homogeneous	689:699	arg1	surface					701:707	homogeneous surface	689:707	homogeneous surface	689:707	Scanning electron micrograph of the tensile fracture showed homogeneous surface with formation of cleavage plane.
28249765	3	29	theme	biodegradation	456:469	arg1	properties					471:480	their mechanical, thermal and biodegradation properties	426:480	their mechanical, thermal and biodegradation properties	426:480	The TPSA/seaweed composites were characterized for their mechanical, thermal and biodegradation properties.
28249765	0	30	theme	seaweed	10:16	arg1	Effect					0:5	Effect	0:5	Effect of seaweed on mechanical, thermal, and biodegradation properties of thermoplastic sugar palm starch/agar composites.	0:122	Effect of seaweed on mechanical, thermal, and biodegradation properties of thermoplastic sugar palm starch/agar composites.
28249765	0	31	theme	composites	112:121	arg1	properties					61:70	mechanical, thermal, and biodegradation properties	21:70	mechanical, thermal, and biodegradation properties of thermoplastic sugar palm starch/agar composites	21:121	Effect of seaweed on mechanical, thermal, and biodegradation properties of thermoplastic sugar palm starch/agar composites.
28249765	1	32	theme	thermoplastic	187:199	arg1	blend					231:235	thermoplastic sugar palm starch/agar (TPSA) blend	187:235	thermoplastic sugar palm starch/agar (TPSA) blend containing Eucheuma cottonii seaweed waste as biofiller	187:291	The aim of this paper is to investigate the characteristics of thermoplastic sugar palm starch/agar (TPSA) blend containing Eucheuma cottonii seaweed waste as biofiller.
28249765	3	33	theme	thermal	444:450	arg1	properties					471:480	their mechanical, thermal and biodegradation properties	426:480	their mechanical, thermal and biodegradation properties	426:480	The TPSA/seaweed composites were characterized for their mechanical, thermal and biodegradation properties.
28249765	6	34	theme	fracture	673:680	arg1	micrograph					647:656	Scanning electron micrograph	629:656	Scanning electron micrograph of the tensile fracture	629:680	Scanning electron micrograph of the tensile fracture showed homogeneous surface with formation of cleavage plane.
28249765	1	35	theme	sugar	201:205	arg1	blend					231:235	thermoplastic sugar palm starch/agar (TPSA) blend	187:235	thermoplastic sugar palm starch/agar (TPSA) blend containing Eucheuma cottonii seaweed waste as biofiller	187:291	The aim of this paper is to investigate the characteristics of thermoplastic sugar palm starch/agar (TPSA) blend containing Eucheuma cottonii seaweed waste as biofiller.
28249765	0	36	theme	mechanical	21:30	arg1	properties					61:70	mechanical, thermal, and biodegradation properties	21:70	mechanical, thermal, and biodegradation properties of thermoplastic sugar palm starch/agar composites	21:121	Effect of seaweed on mechanical, thermal, and biodegradation properties of thermoplastic sugar palm starch/agar composites.
28249765	7	37	theme	thermal	784:790	arg1	stability					792:800	thermal stability	784:800	thermal stability of the composites	784:818	It is also evident from TGA results that thermal stability of the composites were enhanced with addition of seaweed.
28249765	1	38	theme	palm	207:210	arg1	blend					231:235	thermoplastic sugar palm starch/agar (TPSA) blend	187:235	thermoplastic sugar palm starch/agar (TPSA) blend containing Eucheuma cottonii seaweed waste as biofiller	187:291	The aim of this paper is to investigate the characteristics of thermoplastic sugar palm starch/agar (TPSA) blend containing Eucheuma cottonii seaweed waste as biofiller.
28249765	3	39	theme	TPSA/seaweed	379:390	arg1	composites					392:401	The TPSA/seaweed composites	375:401	The TPSA/seaweed composites	375:401	The TPSA/seaweed composites were characterized for their mechanical, thermal and biodegradation properties.
28249765	0	40	theme	thermal	33:39	arg1	properties					61:70	mechanical, thermal, and biodegradation properties	21:70	mechanical, thermal, and biodegradation properties of thermoplastic sugar palm starch/agar composites	21:121	Effect of seaweed on mechanical, thermal, and biodegradation properties of thermoplastic sugar palm starch/agar composites.
28249765	5	41	theme	tensile	556:562	arg1	properties					586:595	the tensile, flexural, and impact properties	552:595	the tensile, flexural, and impact properties of the TPSA/seaweed composites	552:626	% has significantly improved the tensile, flexural, and impact properties of the TPSA/seaweed composites.
28249765	2	42	theme	melt-mixing	326:336	arg1	pressing					346:353	melt-mixing and hot pressing	326:353	melt-mixing and hot pressing	326:353	The composites were prepared by melt-mixing and hot pressing at 140°C for 10min.
28249765	6	43	theme	tensile	665:671	arg1	fracture					673:680	the tensile fracture	661:680	the tensile fracture	661:680	Scanning electron micrograph of the tensile fracture showed homogeneous surface with formation of cleavage plane.
28249765	1	44	theme	starch/agar	212:222	arg1	blend					231:235	thermoplastic sugar palm starch/agar (TPSA) blend	187:235	thermoplastic sugar palm starch/agar (TPSA) blend containing Eucheuma cottonii seaweed waste as biofiller	187:291	The aim of this paper is to investigate the characteristics of thermoplastic sugar palm starch/agar (TPSA) blend containing Eucheuma cottonii seaweed waste as biofiller.
28249765	7	45	theme	TGA	767:769	arg1	results					771:777	TGA results	767:777	TGA results	767:777	It is also evident from TGA results that thermal stability of the composites were enhanced with addition of seaweed.
28249765	0	46	theme	biodegradation	46:59	arg1	properties					61:70	mechanical, thermal, and biodegradation properties	21:70	mechanical, thermal, and biodegradation properties of thermoplastic sugar palm starch/agar composites	21:121	Effect of seaweed on mechanical, thermal, and biodegradation properties of thermoplastic sugar palm starch/agar composites.
28249765	1	47	contain	containing	237:246	arg2	waste					274:278	Eucheuma cottonii seaweed waste	248:278	Eucheuma cottonii seaweed waste	248:278	The aim of this paper is to investigate the characteristics of thermoplastic sugar palm starch/agar (TPSA) blend containing Eucheuma cottonii seaweed waste as biofiller.
28249765	1	47	contain	containing	237:246	arg1	blend					231:235	thermoplastic sugar palm starch/agar (TPSA) blend	187:235	thermoplastic sugar palm starch/agar (TPSA) blend containing Eucheuma cottonii seaweed waste as biofiller	187:291	The aim of this paper is to investigate the characteristics of thermoplastic sugar palm starch/agar (TPSA) blend containing Eucheuma cottonii seaweed waste as biofiller.
28249765	1	48	theme	TPSA	225:228	arg1	blend					231:235	thermoplastic sugar palm starch/agar (TPSA) blend	187:235	thermoplastic sugar palm starch/agar (TPSA) blend containing Eucheuma cottonii seaweed waste as biofiller	187:291	The aim of this paper is to investigate the characteristics of thermoplastic sugar palm starch/agar (TPSA) blend containing Eucheuma cottonii seaweed waste as biofiller.
28249765	8	49	theme	composites	923:932	arg1	biodegradation					901:914	the biodegradation	897:914	the biodegradation of the composites	897:932	After soil burial for 2 and 4 weeks, the biodegradation of the composites was enhanced with addition of seaweed.
28249765	6	50	theme	electron	638:645	arg1	micrograph					647:656	Scanning electron micrograph	629:656	Scanning electron micrograph of the tensile fracture	629:680	Scanning electron micrograph of the tensile fracture showed homogeneous surface with formation of cleavage plane.
26456290	0	0	theme	efficient	81:89	arg1	removal					98:104	efficient Cr(VI) removal	81:104	efficient Cr(VI) removal	81:104	One pot synthesis of metal ion anchored alginate-gelatin binary biocomposite for efficient Cr(VI) removal.
26456290	7	1	theme	Dubinin-Radushkevich	1328:1347	arg1	isotherms					1355:1363	Freundlich, Langmuir and Dubinin-Radushkevich (D-R) isotherms	1303:1363	Freundlich, Langmuir and Dubinin-Radushkevich (D-R) isotherms	1303:1363	The sorption data was fitted by using Freundlich, Langmuir and Dubinin-Radushkevich (D-R) isotherms.
26456290	2	2	theme	Ca2+	591:594	arg1	ions					611:614	Ca2+, Ce3+ and Zr4+ ions	591:614	Ca2+, Ce3+ and Zr4+ ions	591:614	To overcome the limitations of biopolymers and to improve their properties, the present study was designed in such a way to develop a novel sorbent with enhanced chromium sorption capacity and better stability by synthesizing metal ion cross-linked binary biocomposites using biopolymers like alginate and gelatin cross-linked with Ca2+, Ce3+ and Zr4+ ions namely Ca@AlgGel, Ce@AlgGel and Zr@AlgGel composites.
26456290	2	3	theme	AlgGel	651:656	arg1	composites					658:667	Zr@AlgGel composites	648:667	Zr@AlgGel composites	648:667	To overcome the limitations of biopolymers and to improve their properties, the present study was designed in such a way to develop a novel sorbent with enhanced chromium sorption capacity and better stability by synthesizing metal ion cross-linked binary biocomposites using biopolymers like alginate and gelatin cross-linked with Ca2+, Ce3+ and Zr4+ ions namely Ca@AlgGel, Ce@AlgGel and Zr@AlgGel composites.
26456290	8	4	theme	chromium	1414:1421	arg1	sorption					1423:1430	chromium sorption	1414:1430	chromium sorption	1414:1430	Thermodynamic parameters indicate the nature of chromium sorption.
26456290	8	5	theme	Thermodynamic	1366:1378	arg1	parameters					1380:1389	Thermodynamic parameters	1366:1389	Thermodynamic parameters	1366:1389	Thermodynamic parameters indicate the nature of chromium sorption.
26456290	1	6	theme	sorption	222:229	arg1	capacity					231:238	poor sorption capacity	217:238	poor sorption capacity	217:238	Biopolymers are widely used for the removal of chromium from aqueous medium but it possesses limitations like poor sorption capacity and low stability.
26456290	6	7	theme	composites	1194:1203	arg1	mg/g					1245:1248	19.40, 24.50 and 25.40 mg/g	1222:1248	mg/g	1245:1248	The maximum sorption capacity of Ca@AlgGel, Ce@AlgGel and Zr@AlgGel composites were found to be 19.40, 24.50 and 25.40 mg/g, respectively.
26456290	6	7	theme	composites	1194:1203	arg1	capacity					1147:1154	The maximum sorption capacity	1126:1154	The maximum sorption capacity of Ca@AlgGel, Ce@AlgGel and Zr@AlgGel composites	1126:1203	The maximum sorption capacity of Ca@AlgGel, Ce@AlgGel and Zr@AlgGel composites were found to be 19.40, 24.50 and 25.40 mg/g, respectively.
26456290	9	8	theme	field	1502:1506	arg1	conditions					1508:1517	the field conditions	1498:1517	the field conditions	1498:1517	The suitability of the composite materials was also tested under the field conditions.
26456290	2	9	theme	@	625:625	arg1	AlgGel					626:631	Ca@AlgGel	623:631	Ca@AlgGel	623:631	To overcome the limitations of biopolymers and to improve their properties, the present study was designed in such a way to develop a novel sorbent with enhanced chromium sorption capacity and better stability by synthesizing metal ion cross-linked binary biocomposites using biopolymers like alginate and gelatin cross-linked with Ca2+, Ce3+ and Zr4+ ions namely Ca@AlgGel, Ce@AlgGel and Zr@AlgGel composites.
26456290	0	10	dep	removal	98:104	arg1	VI					94:95	VI	94:95	VI	94:95	One pot synthesis of metal ion anchored alginate-gelatin binary biocomposite for efficient Cr(VI) removal.
26456290	5	11	theme	chromium	1086:1093	arg1	concentration					1095:1107	initial chromium concentration	1078:1107	initial chromium concentration	1078:1107	The sorption process was optimized by varying the influencing aspects like contact time, dosage, presence of common ions, pH, initial chromium concentration and temperature.
26456290	2	12	theme	ion	491:493	arg1	biocomposites					515:527	metal ion cross-linked binary biocomposites	485:527	metal ion cross-linked binary biocomposites using biopolymers like alginate and gelatin cross-linked with Ca2+, Ce3+ and Zr4+ ions namely Ca@AlgGel, Ce@AlgGel and Zr@AlgGel composites	485:667	To overcome the limitations of biopolymers and to improve their properties, the present study was designed in such a way to develop a novel sorbent with enhanced chromium sorption capacity and better stability by synthesizing metal ion cross-linked binary biocomposites using biopolymers like alginate and gelatin cross-linked with Ca2+, Ce3+ and Zr4+ ions namely Ca@AlgGel, Ce@AlgGel and Zr@AlgGel composites.
26456290	7	13	theme	Langmuir	1315:1322	arg1	isotherms					1355:1363	Freundlich, Langmuir and Dubinin-Radushkevich (D-R) isotherms	1303:1363	Freundlich, Langmuir and Dubinin-Radushkevich (D-R) isotherms	1303:1363	The sorption data was fitted by using Freundlich, Langmuir and Dubinin-Radushkevich (D-R) isotherms.
26456290	6	14	theme	AlgGel	1162:1167	arg1	mg/g					1245:1248	19.40, 24.50 and 25.40 mg/g	1222:1248	mg/g	1245:1248	The maximum sorption capacity of Ca@AlgGel, Ce@AlgGel and Zr@AlgGel composites were found to be 19.40, 24.50 and 25.40 mg/g, respectively.
26456290	6	14	theme	AlgGel	1162:1167	arg1	capacity					1147:1154	The maximum sorption capacity	1126:1154	The maximum sorption capacity of Ca@AlgGel, Ce@AlgGel and Zr@AlgGel composites	1126:1203	The maximum sorption capacity of Ca@AlgGel, Ce@AlgGel and Zr@AlgGel composites were found to be 19.40, 24.50 and 25.40 mg/g, respectively.
26456290	2	15	theme	Ca	623:624	arg1	AlgGel					626:631	Ca@AlgGel	623:631	Ca@AlgGel	623:631	To overcome the limitations of biopolymers and to improve their properties, the present study was designed in such a way to develop a novel sorbent with enhanced chromium sorption capacity and better stability by synthesizing metal ion cross-linked binary biocomposites using biopolymers like alginate and gelatin cross-linked with Ca2+, Ce3+ and Zr4+ ions namely Ca@AlgGel, Ce@AlgGel and Zr@AlgGel composites.
26456290	8	16	theme	sorption	1423:1430	arg1	nature					1404:1409	the nature	1400:1409	the nature of chromium sorption	1400:1430	Thermodynamic parameters indicate the nature of chromium sorption.
26456290	0	17	theme	Cr	91:92	arg1	removal					98:104	efficient Cr(VI) removal	81:104	efficient Cr(VI) removal	81:104	One pot synthesis of metal ion anchored alginate-gelatin binary biocomposite for efficient Cr(VI) removal.
26456290	6	18	theme	@	1172:1172	arg1	AlgGel					1173:1178	Ce@AlgGel	1170:1178	Ce@AlgGel	1170:1178	The maximum sorption capacity of Ca@AlgGel, Ce@AlgGel and Zr@AlgGel composites were found to be 19.40, 24.50 and 25.40 mg/g, respectively.
26456290	6	19	theme	@	1161:1161	arg1	AlgGel					1162:1167	Ca@AlgGel	1159:1167	Ca@AlgGel	1159:1167	The maximum sorption capacity of Ca@AlgGel, Ce@AlgGel and Zr@AlgGel composites were found to be 19.40, 24.50 and 25.40 mg/g, respectively.
26456290	9	20	theme	composite	1456:1464	arg1	materials					1466:1474	the composite materials	1452:1474	the composite materials	1452:1474	The suitability of the composite materials was also tested under the field conditions.
26456290	1	21	theme	low	244:246	arg1	stability					248:256	low stability	244:256	low stability	244:256	Biopolymers are widely used for the removal of chromium from aqueous medium but it possesses limitations like poor sorption capacity and low stability.
26456290	5	22	theme	influencing	1002:1012	arg1	aspects					1014:1020	the influencing aspects	998:1020	the influencing aspects like contact time, dosage, presence of common ions, pH, initial chromium concentration and temperature	998:1123	The sorption process was optimized by varying the influencing aspects like contact time, dosage, presence of common ions, pH, initial chromium concentration and temperature.
26456290	2	23	theme	Zr4+	606:609	arg1	ions					611:614	Ca2+, Ce3+ and Zr4+ ions	591:614	Ca2+, Ce3+ and Zr4+ ions	591:614	To overcome the limitations of biopolymers and to improve their properties, the present study was designed in such a way to develop a novel sorbent with enhanced chromium sorption capacity and better stability by synthesizing metal ion cross-linked binary biocomposites using biopolymers like alginate and gelatin cross-linked with Ca2+, Ce3+ and Zr4+ ions namely Ca@AlgGel, Ce@AlgGel and Zr@AlgGel composites.
26456290	1	24	used	used	130:133	arg2	Biopolymers					107:117	Biopolymers	107:117	Biopolymers	107:117	Biopolymers are widely used for the removal of chromium from aqueous medium but it possesses limitations like poor sorption capacity and low stability.
26456290	2	25	link	cross-linked	495:506	arg1	biocomposites					515:527	metal ion cross-linked binary biocomposites	485:527	metal ion cross-linked binary biocomposites using biopolymers like alginate and gelatin cross-linked with Ca2+, Ce3+ and Zr4+ ions namely Ca@AlgGel, Ce@AlgGel and Zr@AlgGel composites	485:667	To overcome the limitations of biopolymers and to improve their properties, the present study was designed in such a way to develop a novel sorbent with enhanced chromium sorption capacity and better stability by synthesizing metal ion cross-linked binary biocomposites using biopolymers like alginate and gelatin cross-linked with Ca2+, Ce3+ and Zr4+ ions namely Ca@AlgGel, Ce@AlgGel and Zr@AlgGel composites.
26456290	2	26	theme	sorption	430:437	arg1	capacity					439:446	enhanced chromium sorption capacity	412:446	enhanced chromium sorption capacity	412:446	To overcome the limitations of biopolymers and to improve their properties, the present study was designed in such a way to develop a novel sorbent with enhanced chromium sorption capacity and better stability by synthesizing metal ion cross-linked binary biocomposites using biopolymers like alginate and gelatin cross-linked with Ca2+, Ce3+ and Zr4+ ions namely Ca@AlgGel, Ce@AlgGel and Zr@AlgGel composites.
26456290	2	27	theme	@	636:636	arg1	AlgGel					637:642	Ce@AlgGel	634:642	Ce@AlgGel	634:642	To overcome the limitations of biopolymers and to improve their properties, the present study was designed in such a way to develop a novel sorbent with enhanced chromium sorption capacity and better stability by synthesizing metal ion cross-linked binary biocomposites using biopolymers like alginate and gelatin cross-linked with Ca2+, Ce3+ and Zr4+ ions namely Ca@AlgGel, Ce@AlgGel and Zr@AlgGel composites.
26456290	7	28	theme	sorption	1269:1276	arg1	data					1278:1281	The sorption data	1265:1281	The sorption data	1265:1281	The sorption data was fitted by using Freundlich, Langmuir and Dubinin-Radushkevich (D-R) isotherms.
26456290	4	29	theme	chromium	874:881	arg1	studies					891:897	The chromium removal studies	870:897	The chromium removal studies of the biocomposites	870:918	The chromium removal studies of the biocomposites were carried out in batch mode.
26456290	0	30	theme	pot	4:6	arg1	synthesis					8:16	One pot synthesis	0:16	One pot synthesis of metal ion	0:29	One pot synthesis of metal ion anchored alginate-gelatin binary biocomposite for efficient Cr(VI) removal.
26456290	6	31	theme	Ce	1170:1171	arg1	AlgGel					1173:1178	Ce@AlgGel	1170:1178	Ce@AlgGel	1170:1178	The maximum sorption capacity of Ca@AlgGel, Ce@AlgGel and Zr@AlgGel composites were found to be 19.40, 24.50 and 25.40 mg/g, respectively.
26456290	2	32	theme	Ce3+	597:600	arg1	ions					611:614	Ca2+, Ce3+ and Zr4+ ions	591:614	Ca2+, Ce3+ and Zr4+ ions	591:614	To overcome the limitations of biopolymers and to improve their properties, the present study was designed in such a way to develop a novel sorbent with enhanced chromium sorption capacity and better stability by synthesizing metal ion cross-linked binary biocomposites using biopolymers like alginate and gelatin cross-linked with Ca2+, Ce3+ and Zr4+ ions namely Ca@AlgGel, Ce@AlgGel and Zr@AlgGel composites.
26456290	2	33	theme	Ce	634:635	arg1	AlgGel					637:642	Ce@AlgGel	634:642	Ce@AlgGel	634:642	To overcome the limitations of biopolymers and to improve their properties, the present study was designed in such a way to develop a novel sorbent with enhanced chromium sorption capacity and better stability by synthesizing metal ion cross-linked binary biocomposites using biopolymers like alginate and gelatin cross-linked with Ca2+, Ce3+ and Zr4+ ions namely Ca@AlgGel, Ce@AlgGel and Zr@AlgGel composites.
26456290	2	34	theme	biopolymers	290:300	arg1	limitations					275:285	the limitations	271:285	the limitations of biopolymers	271:300	To overcome the limitations of biopolymers and to improve their properties, the present study was designed in such a way to develop a novel sorbent with enhanced chromium sorption capacity and better stability by synthesizing metal ion cross-linked binary biocomposites using biopolymers like alginate and gelatin cross-linked with Ca2+, Ce3+ and Zr4+ ions namely Ca@AlgGel, Ce@AlgGel and Zr@AlgGel composites.
26456290	7	35	theme	Freundlich	1303:1312	arg1	isotherms					1355:1363	Freundlich, Langmuir and Dubinin-Radushkevich (D-R) isotherms	1303:1363	Freundlich, Langmuir and Dubinin-Radushkevich (D-R) isotherms	1303:1363	The sorption data was fitted by using Freundlich, Langmuir and Dubinin-Radushkevich (D-R) isotherms.
26456290	1	36	theme	chromium	154:161	arg1	removal					143:149	the removal	139:149	the removal of chromium from aqueous medium	139:181	Biopolymers are widely used for the removal of chromium from aqueous medium but it possesses limitations like poor sorption capacity and low stability.
26456290	2	37	theme	novel	393:397	arg1	sorbent					399:405	a novel sorbent	391:405	a novel sorbent with enhanced chromium sorption capacity and better stability	391:467	To overcome the limitations of biopolymers and to improve their properties, the present study was designed in such a way to develop a novel sorbent with enhanced chromium sorption capacity and better stability by synthesizing metal ion cross-linked binary biocomposites using biopolymers like alginate and gelatin cross-linked with Ca2+, Ce3+ and Zr4+ ions namely Ca@AlgGel, Ce@AlgGel and Zr@AlgGel composites.
26456290	5	38	theme	ions	1068:1071	arg1	concentration					1095:1107	initial chromium concentration	1078:1107	initial chromium concentration	1078:1107	The sorption process was optimized by varying the influencing aspects like contact time, dosage, presence of common ions, pH, initial chromium concentration and temperature.
26456290	5	38	theme	ions	1068:1071	arg1	time					1035:1038	contact time	1027:1038	contact time	1027:1038	The sorption process was optimized by varying the influencing aspects like contact time, dosage, presence of common ions, pH, initial chromium concentration and temperature.
26456290	5	38	theme	ions	1068:1071	arg1	dosage					1041:1046	dosage	1041:1046	dosage	1041:1046	The sorption process was optimized by varying the influencing aspects like contact time, dosage, presence of common ions, pH, initial chromium concentration and temperature.
26456290	5	38	theme	ions	1068:1071	arg1	pH					1074:1075	pH	1074:1075	pH	1074:1075	The sorption process was optimized by varying the influencing aspects like contact time, dosage, presence of common ions, pH, initial chromium concentration and temperature.
26456290	5	38	theme	ions	1068:1071	arg1	temperature					1113:1123	temperature	1113:1123	temperature	1113:1123	The sorption process was optimized by varying the influencing aspects like contact time, dosage, presence of common ions, pH, initial chromium concentration and temperature.
26456290	5	38	theme	ions	1068:1071	arg1	presence					1049:1056	presence	1049:1056	presence of common ions	1049:1071	The sorption process was optimized by varying the influencing aspects like contact time, dosage, presence of common ions, pH, initial chromium concentration and temperature.
26456290	6	39	theme	sorption	1138:1145	arg1	mg/g					1245:1248	19.40, 24.50 and 25.40 mg/g	1222:1248	mg/g	1245:1248	The maximum sorption capacity of Ca@AlgGel, Ce@AlgGel and Zr@AlgGel composites were found to be 19.40, 24.50 and 25.40 mg/g, respectively.
26456290	6	39	theme	sorption	1138:1145	arg1	capacity					1147:1154	The maximum sorption capacity	1126:1154	The maximum sorption capacity of Ca@AlgGel, Ce@AlgGel and Zr@AlgGel composites	1126:1203	The maximum sorption capacity of Ca@AlgGel, Ce@AlgGel and Zr@AlgGel composites were found to be 19.40, 24.50 and 25.40 mg/g, respectively.
26456290	6	40	theme	Ca	1159:1160	arg1	AlgGel					1162:1167	Ca@AlgGel	1159:1167	Ca@AlgGel	1159:1167	The maximum sorption capacity of Ca@AlgGel, Ce@AlgGel and Zr@AlgGel composites were found to be 19.40, 24.50 and 25.40 mg/g, respectively.
26456290	9	41	theme	materials	1466:1474	arg1	suitability					1437:1447	The suitability	1433:1447	The suitability of the composite materials	1433:1474	The suitability of the composite materials was also tested under the field conditions.
26456290	1	42	theme	aqueous	168:174	arg1	medium					176:181	aqueous medium	168:181	aqueous medium	168:181	Biopolymers are widely used for the removal of chromium from aqueous medium but it possesses limitations like poor sorption capacity and low stability.
26456290	4	43	theme	batch	940:944	arg1	mode					946:949	batch mode	940:949	batch mode	940:949	The chromium removal studies of the biocomposites were carried out in batch mode.
26456290	3	44	theme	functional	674:683	arg1	composites					790:799	the composites	786:799	the composites	786:799	The functional groups, agglomeration, surface area, surface morphology, elemental analysis and thermal stability of the composites were investigated by FTIR, TEM, BET, SEM with EDAX and TGA analysis.
26456290	3	44	theme	functional	674:683	arg1	area					716:719	surface area	708:719	surface area	708:719	The functional groups, agglomeration, surface area, surface morphology, elemental analysis and thermal stability of the composites were investigated by FTIR, TEM, BET, SEM with EDAX and TGA analysis.
26456290	3	44	theme	functional	674:683	arg1	analysis					752:759	elemental analysis	742:759	elemental analysis	742:759	The functional groups, agglomeration, surface area, surface morphology, elemental analysis and thermal stability of the composites were investigated by FTIR, TEM, BET, SEM with EDAX and TGA analysis.
26456290	3	44	theme	functional	674:683	arg1	groups					685:690	The functional groups	670:690	The functional groups	670:690	The functional groups, agglomeration, surface area, surface morphology, elemental analysis and thermal stability of the composites were investigated by FTIR, TEM, BET, SEM with EDAX and TGA analysis.
26456290	3	44	theme	functional	674:683	arg1	stability					773:781	thermal stability	765:781	thermal stability	765:781	The functional groups, agglomeration, surface area, surface morphology, elemental analysis and thermal stability of the composites were investigated by FTIR, TEM, BET, SEM with EDAX and TGA analysis.
26456290	3	44	theme	functional	674:683	arg1	agglomeration					693:705	agglomeration	693:705	agglomeration	693:705	The functional groups, agglomeration, surface area, surface morphology, elemental analysis and thermal stability of the composites were investigated by FTIR, TEM, BET, SEM with EDAX and TGA analysis.
26456290	3	44	theme	functional	674:683	arg1	morphology					730:739	surface morphology	722:739	surface morphology	722:739	The functional groups, agglomeration, surface area, surface morphology, elemental analysis and thermal stability of the composites were investigated by FTIR, TEM, BET, SEM with EDAX and TGA analysis.
26456290	3	45	theme	TGA	856:858	arg1	analysis					860:867	TGA analysis	856:867	TGA analysis	856:867	The functional groups, agglomeration, surface area, surface morphology, elemental analysis and thermal stability of the composites were investigated by FTIR, TEM, BET, SEM with EDAX and TGA analysis.
26456290	2	46	theme	enhanced	412:419	arg1	capacity					439:446	enhanced chromium sorption capacity	412:446	enhanced chromium sorption capacity	412:446	To overcome the limitations of biopolymers and to improve their properties, the present study was designed in such a way to develop a novel sorbent with enhanced chromium sorption capacity and better stability by synthesizing metal ion cross-linked binary biocomposites using biopolymers like alginate and gelatin cross-linked with Ca2+, Ce3+ and Zr4+ ions namely Ca@AlgGel, Ce@AlgGel and Zr@AlgGel composites.
26456290	0	47	theme	ion	27:29	arg1	synthesis					8:16	One pot synthesis	0:16	One pot synthesis of metal ion	0:29	One pot synthesis of metal ion anchored alginate-gelatin binary biocomposite for efficient Cr(VI) removal.
26456290	2	48	theme	binary	508:513	arg1	biocomposites					515:527	metal ion cross-linked binary biocomposites	485:527	metal ion cross-linked binary biocomposites using biopolymers like alginate and gelatin cross-linked with Ca2+, Ce3+ and Zr4+ ions namely Ca@AlgGel, Ce@AlgGel and Zr@AlgGel composites	485:667	To overcome the limitations of biopolymers and to improve their properties, the present study was designed in such a way to develop a novel sorbent with enhanced chromium sorption capacity and better stability by synthesizing metal ion cross-linked binary biocomposites using biopolymers like alginate and gelatin cross-linked with Ca2+, Ce3+ and Zr4+ ions namely Ca@AlgGel, Ce@AlgGel and Zr@AlgGel composites.
26456290	6	49	theme	maximum	1130:1136	arg1	mg/g					1245:1248	19.40, 24.50 and 25.40 mg/g	1222:1248	mg/g	1245:1248	The maximum sorption capacity of Ca@AlgGel, Ce@AlgGel and Zr@AlgGel composites were found to be 19.40, 24.50 and 25.40 mg/g, respectively.
26456290	6	49	theme	maximum	1130:1136	arg1	capacity					1147:1154	The maximum sorption capacity	1126:1154	The maximum sorption capacity of Ca@AlgGel, Ce@AlgGel and Zr@AlgGel composites	1126:1203	The maximum sorption capacity of Ca@AlgGel, Ce@AlgGel and Zr@AlgGel composites were found to be 19.40, 24.50 and 25.40 mg/g, respectively.
26456290	0	50	theme	metal	21:25	arg1	ion					27:29	metal ion	21:29	metal ion	21:29	One pot synthesis of metal ion anchored alginate-gelatin binary biocomposite for efficient Cr(VI) removal.
26456290	5	51	theme	initial	1078:1084	arg1	concentration					1095:1107	initial chromium concentration	1078:1107	initial chromium concentration	1078:1107	The sorption process was optimized by varying the influencing aspects like contact time, dosage, presence of common ions, pH, initial chromium concentration and temperature.
26456290	2	52	theme	chromium	421:428	arg1	capacity					439:446	enhanced chromium sorption capacity	412:446	enhanced chromium sorption capacity	412:446	To overcome the limitations of biopolymers and to improve their properties, the present study was designed in such a way to develop a novel sorbent with enhanced chromium sorption capacity and better stability by synthesizing metal ion cross-linked binary biocomposites using biopolymers like alginate and gelatin cross-linked with Ca2+, Ce3+ and Zr4+ ions namely Ca@AlgGel, Ce@AlgGel and Zr@AlgGel composites.
26456290	6	53	theme	AlgGel	1187:1192	arg1	composites					1194:1203	Zr@AlgGel composites	1184:1203	Zr@AlgGel composites	1184:1203	The maximum sorption capacity of Ca@AlgGel, Ce@AlgGel and Zr@AlgGel composites were found to be 19.40, 24.50 and 25.40 mg/g, respectively.
26456290	2	54	theme	@	650:650	arg1	composites					658:667	Zr@AlgGel composites	648:667	Zr@AlgGel composites	648:667	To overcome the limitations of biopolymers and to improve their properties, the present study was designed in such a way to develop a novel sorbent with enhanced chromium sorption capacity and better stability by synthesizing metal ion cross-linked binary biocomposites using biopolymers like alginate and gelatin cross-linked with Ca2+, Ce3+ and Zr4+ ions namely Ca@AlgGel, Ce@AlgGel and Zr@AlgGel composites.
26456290	5	55	theme	common	1061:1066	arg1	ions					1068:1071	common ions	1061:1071	common ions	1061:1071	The sorption process was optimized by varying the influencing aspects like contact time, dosage, presence of common ions, pH, initial chromium concentration and temperature.
26456290	0	56	theme	alginate-gelatin	40:55	arg1	biocomposite					64:75	alginate-gelatin binary biocomposite	40:75	alginate-gelatin binary biocomposite for efficient Cr(VI) removal	40:104	One pot synthesis of metal ion anchored alginate-gelatin binary biocomposite for efficient Cr(VI) removal.
26456290	6	57	theme	19.40	1222:1226	arg1	mg/g					1245:1248	19.40, 24.50 and 25.40 mg/g	1222:1248	mg/g	1245:1248	The maximum sorption capacity of Ca@AlgGel, Ce@AlgGel and Zr@AlgGel composites were found to be 19.40, 24.50 and 25.40 mg/g, respectively.
26456290	6	57	theme	19.40	1222:1226	arg1	capacity					1147:1154	The maximum sorption capacity	1126:1154	The maximum sorption capacity of Ca@AlgGel, Ce@AlgGel and Zr@AlgGel composites	1126:1203	The maximum sorption capacity of Ca@AlgGel, Ce@AlgGel and Zr@AlgGel composites were found to be 19.40, 24.50 and 25.40 mg/g, respectively.
26456290	6	58	theme	Zr	1184:1185	arg1	composites					1194:1203	Zr@AlgGel composites	1184:1203	Zr@AlgGel composites	1184:1203	The maximum sorption capacity of Ca@AlgGel, Ce@AlgGel and Zr@AlgGel composites were found to be 19.40, 24.50 and 25.40 mg/g, respectively.
26456290	2	59	theme	better	452:457	arg1	stability					459:467	better stability	452:467	better stability	452:467	To overcome the limitations of biopolymers and to improve their properties, the present study was designed in such a way to develop a novel sorbent with enhanced chromium sorption capacity and better stability by synthesizing metal ion cross-linked binary biocomposites using biopolymers like alginate and gelatin cross-linked with Ca2+, Ce3+ and Zr4+ ions namely Ca@AlgGel, Ce@AlgGel and Zr@AlgGel composites.
26456290	2	60	theme	metal	485:489	arg1	biocomposites					515:527	metal ion cross-linked binary biocomposites	485:527	metal ion cross-linked binary biocomposites using biopolymers like alginate and gelatin cross-linked with Ca2+, Ce3+ and Zr4+ ions namely Ca@AlgGel, Ce@AlgGel and Zr@AlgGel composites	485:667	To overcome the limitations of biopolymers and to improve their properties, the present study was designed in such a way to develop a novel sorbent with enhanced chromium sorption capacity and better stability by synthesizing metal ion cross-linked binary biocomposites using biopolymers like alginate and gelatin cross-linked with Ca2+, Ce3+ and Zr4+ ions namely Ca@AlgGel, Ce@AlgGel and Zr@AlgGel composites.
26456290	2	61	theme	Zr	648:649	arg1	composites					658:667	Zr@AlgGel composites	648:667	Zr@AlgGel composites	648:667	To overcome the limitations of biopolymers and to improve their properties, the present study was designed in such a way to develop a novel sorbent with enhanced chromium sorption capacity and better stability by synthesizing metal ion cross-linked binary biocomposites using biopolymers like alginate and gelatin cross-linked with Ca2+, Ce3+ and Zr4+ ions namely Ca@AlgGel, Ce@AlgGel and Zr@AlgGel composites.
26456290	2	62	theme	cross-linked	495:506	arg1	biocomposites					515:527	metal ion cross-linked binary biocomposites	485:527	metal ion cross-linked binary biocomposites using biopolymers like alginate and gelatin cross-linked with Ca2+, Ce3+ and Zr4+ ions namely Ca@AlgGel, Ce@AlgGel and Zr@AlgGel composites	485:667	To overcome the limitations of biopolymers and to improve their properties, the present study was designed in such a way to develop a novel sorbent with enhanced chromium sorption capacity and better stability by synthesizing metal ion cross-linked binary biocomposites using biopolymers like alginate and gelatin cross-linked with Ca2+, Ce3+ and Zr4+ ions namely Ca@AlgGel, Ce@AlgGel and Zr@AlgGel composites.
26456290	1	63	contain	possesses	190:198	arg2	limitations					200:210	limitations	200:210	limitations like poor sorption capacity and low stability	200:256	Biopolymers are widely used for the removal of chromium from aqueous medium but it possesses limitations like poor sorption capacity and low stability.
26456290	1	63	contain	possesses	190:198	arg1	it					187:188	it	187:188	it	187:188	Biopolymers are widely used for the removal of chromium from aqueous medium but it possesses limitations like poor sorption capacity and low stability.
26456290	2	64	with	sorbent	399:405	arg1	capacity					439:446	enhanced chromium sorption capacity	412:446	enhanced chromium sorption capacity	412:446	To overcome the limitations of biopolymers and to improve their properties, the present study was designed in such a way to develop a novel sorbent with enhanced chromium sorption capacity and better stability by synthesizing metal ion cross-linked binary biocomposites using biopolymers like alginate and gelatin cross-linked with Ca2+, Ce3+ and Zr4+ ions namely Ca@AlgGel, Ce@AlgGel and Zr@AlgGel composites.
26456290	2	64	with	sorbent	399:405	arg1	stability					459:467	better stability	452:467	better stability	452:467	To overcome the limitations of biopolymers and to improve their properties, the present study was designed in such a way to develop a novel sorbent with enhanced chromium sorption capacity and better stability by synthesizing metal ion cross-linked binary biocomposites using biopolymers like alginate and gelatin cross-linked with Ca2+, Ce3+ and Zr4+ ions namely Ca@AlgGel, Ce@AlgGel and Zr@AlgGel composites.
26456290	3	65	theme	composites	790:799	arg1	composites					790:799	the composites	786:799	the composites	786:799	The functional groups, agglomeration, surface area, surface morphology, elemental analysis and thermal stability of the composites were investigated by FTIR, TEM, BET, SEM with EDAX and TGA analysis.
26456290	3	65	theme	composites	790:799	arg1	area					716:719	surface area	708:719	surface area	708:719	The functional groups, agglomeration, surface area, surface morphology, elemental analysis and thermal stability of the composites were investigated by FTIR, TEM, BET, SEM with EDAX and TGA analysis.
26456290	3	65	theme	composites	790:799	arg1	analysis					752:759	elemental analysis	742:759	elemental analysis	742:759	The functional groups, agglomeration, surface area, surface morphology, elemental analysis and thermal stability of the composites were investigated by FTIR, TEM, BET, SEM with EDAX and TGA analysis.
26456290	3	65	theme	composites	790:799	arg1	groups					685:690	The functional groups	670:690	The functional groups	670:690	The functional groups, agglomeration, surface area, surface morphology, elemental analysis and thermal stability of the composites were investigated by FTIR, TEM, BET, SEM with EDAX and TGA analysis.
26456290	3	65	theme	composites	790:799	arg1	stability					773:781	thermal stability	765:781	thermal stability	765:781	The functional groups, agglomeration, surface area, surface morphology, elemental analysis and thermal stability of the composites were investigated by FTIR, TEM, BET, SEM with EDAX and TGA analysis.
26456290	3	65	theme	composites	790:799	arg1	agglomeration					693:705	agglomeration	693:705	agglomeration	693:705	The functional groups, agglomeration, surface area, surface morphology, elemental analysis and thermal stability of the composites were investigated by FTIR, TEM, BET, SEM with EDAX and TGA analysis.
26456290	3	65	theme	composites	790:799	arg1	morphology					730:739	surface morphology	722:739	surface morphology	722:739	The functional groups, agglomeration, surface area, surface morphology, elemental analysis and thermal stability of the composites were investigated by FTIR, TEM, BET, SEM with EDAX and TGA analysis.
26456290	3	66	theme	surface	708:714	arg1	composites					790:799	the composites	786:799	the composites	786:799	The functional groups, agglomeration, surface area, surface morphology, elemental analysis and thermal stability of the composites were investigated by FTIR, TEM, BET, SEM with EDAX and TGA analysis.
26456290	3	66	theme	surface	708:714	arg1	area					716:719	surface area	708:719	surface area	708:719	The functional groups, agglomeration, surface area, surface morphology, elemental analysis and thermal stability of the composites were investigated by FTIR, TEM, BET, SEM with EDAX and TGA analysis.
26456290	3	66	theme	surface	708:714	arg1	groups					685:690	The functional groups	670:690	The functional groups	670:690	The functional groups, agglomeration, surface area, surface morphology, elemental analysis and thermal stability of the composites were investigated by FTIR, TEM, BET, SEM with EDAX and TGA analysis.
26456290	6	67	theme	AlgGel	1173:1178	arg1	mg/g					1245:1248	19.40, 24.50 and 25.40 mg/g	1222:1248	mg/g	1245:1248	The maximum sorption capacity of Ca@AlgGel, Ce@AlgGel and Zr@AlgGel composites were found to be 19.40, 24.50 and 25.40 mg/g, respectively.
26456290	6	67	theme	AlgGel	1173:1178	arg1	capacity					1147:1154	The maximum sorption capacity	1126:1154	The maximum sorption capacity of Ca@AlgGel, Ce@AlgGel and Zr@AlgGel composites	1126:1203	The maximum sorption capacity of Ca@AlgGel, Ce@AlgGel and Zr@AlgGel composites were found to be 19.40, 24.50 and 25.40 mg/g, respectively.
26456290	1	68	from	medium	176:181	arg1	removal					143:149	the removal	139:149	the removal of chromium from aqueous medium	139:181	Biopolymers are widely used for the removal of chromium from aqueous medium but it possesses limitations like poor sorption capacity and low stability.
26456290	6	69	theme	@	1186:1186	arg1	composites					1194:1203	Zr@AlgGel composites	1184:1203	Zr@AlgGel composites	1184:1203	The maximum sorption capacity of Ca@AlgGel, Ce@AlgGel and Zr@AlgGel composites were found to be 19.40, 24.50 and 25.40 mg/g, respectively.
26456290	4	70	theme	biocomposites	906:918	arg1	studies					891:897	The chromium removal studies	870:897	The chromium removal studies of the biocomposites	870:918	The chromium removal studies of the biocomposites were carried out in batch mode.
26456290	5	71	theme	sorption	956:963	arg1	process					965:971	The sorption process	952:971	The sorption process	952:971	The sorption process was optimized by varying the influencing aspects like contact time, dosage, presence of common ions, pH, initial chromium concentration and temperature.
26456290	6	72	theme	25.40	1239:1243	arg1	mg/g					1245:1248	19.40, 24.50 and 25.40 mg/g	1222:1248	mg/g	1245:1248	The maximum sorption capacity of Ca@AlgGel, Ce@AlgGel and Zr@AlgGel composites were found to be 19.40, 24.50 and 25.40 mg/g, respectively.
26456290	6	72	theme	25.40	1239:1243	arg1	capacity					1147:1154	The maximum sorption capacity	1126:1154	The maximum sorption capacity of Ca@AlgGel, Ce@AlgGel and Zr@AlgGel composites	1126:1203	The maximum sorption capacity of Ca@AlgGel, Ce@AlgGel and Zr@AlgGel composites were found to be 19.40, 24.50 and 25.40 mg/g, respectively.
26456290	3	73	theme	thermal	765:771	arg1	composites					790:799	the composites	786:799	the composites	786:799	The functional groups, agglomeration, surface area, surface morphology, elemental analysis and thermal stability of the composites were investigated by FTIR, TEM, BET, SEM with EDAX and TGA analysis.
26456290	3	73	theme	thermal	765:771	arg1	groups					685:690	The functional groups	670:690	The functional groups	670:690	The functional groups, agglomeration, surface area, surface morphology, elemental analysis and thermal stability of the composites were investigated by FTIR, TEM, BET, SEM with EDAX and TGA analysis.
26456290	3	73	theme	thermal	765:771	arg1	stability					773:781	thermal stability	765:781	thermal stability	765:781	The functional groups, agglomeration, surface area, surface morphology, elemental analysis and thermal stability of the composites were investigated by FTIR, TEM, BET, SEM with EDAX and TGA analysis.
26456290	3	74	theme	elemental	742:750	arg1	composites					790:799	the composites	786:799	the composites	786:799	The functional groups, agglomeration, surface area, surface morphology, elemental analysis and thermal stability of the composites were investigated by FTIR, TEM, BET, SEM with EDAX and TGA analysis.
26456290	3	74	theme	elemental	742:750	arg1	groups					685:690	The functional groups	670:690	The functional groups	670:690	The functional groups, agglomeration, surface area, surface morphology, elemental analysis and thermal stability of the composites were investigated by FTIR, TEM, BET, SEM with EDAX and TGA analysis.
26456290	3	74	theme	elemental	742:750	arg1	analysis					752:759	elemental analysis	742:759	elemental analysis	742:759	The functional groups, agglomeration, surface area, surface morphology, elemental analysis and thermal stability of the composites were investigated by FTIR, TEM, BET, SEM with EDAX and TGA analysis.
26456290	4	75	theme	removal	883:889	arg1	studies					891:897	The chromium removal studies	870:897	The chromium removal studies of the biocomposites	870:918	The chromium removal studies of the biocomposites were carried out in batch mode.
26456290	2	76	theme	present	339:345	arg1	study					347:351	the present study	335:351	the present study	335:351	To overcome the limitations of biopolymers and to improve their properties, the present study was designed in such a way to develop a novel sorbent with enhanced chromium sorption capacity and better stability by synthesizing metal ion cross-linked binary biocomposites using biopolymers like alginate and gelatin cross-linked with Ca2+, Ce3+ and Zr4+ ions namely Ca@AlgGel, Ce@AlgGel and Zr@AlgGel composites.
26456290	3	77	theme	surface	722:728	arg1	composites					790:799	the composites	786:799	the composites	786:799	The functional groups, agglomeration, surface area, surface morphology, elemental analysis and thermal stability of the composites were investigated by FTIR, TEM, BET, SEM with EDAX and TGA analysis.
26456290	3	77	theme	surface	722:728	arg1	morphology					730:739	surface morphology	722:739	surface morphology	722:739	The functional groups, agglomeration, surface area, surface morphology, elemental analysis and thermal stability of the composites were investigated by FTIR, TEM, BET, SEM with EDAX and TGA analysis.
26456290	3	77	theme	surface	722:728	arg1	groups					685:690	The functional groups	670:690	The functional groups	670:690	The functional groups, agglomeration, surface area, surface morphology, elemental analysis and thermal stability of the composites were investigated by FTIR, TEM, BET, SEM with EDAX and TGA analysis.
26456290	0	78	theme	binary	57:62	arg1	biocomposite					64:75	alginate-gelatin binary biocomposite	40:75	alginate-gelatin binary biocomposite for efficient Cr(VI) removal	40:104	One pot synthesis of metal ion anchored alginate-gelatin binary biocomposite for efficient Cr(VI) removal.
26456290	5	79	theme	contact	1027:1033	arg1	time					1035:1038	contact time	1027:1038	contact time	1027:1038	The sorption process was optimized by varying the influencing aspects like contact time, dosage, presence of common ions, pH, initial chromium concentration and temperature.
26456290	1	80	theme	poor	217:220	arg1	capacity					231:238	poor sorption capacity	217:238	poor sorption capacity	217:238	Biopolymers are widely used for the removal of chromium from aqueous medium but it possesses limitations like poor sorption capacity and low stability.
26123432	2	0	theme	downstream	482:491	arg1	purification					493:504	the downstream purification	478:504	the downstream purification of N. meningitidis serogroup A (MenA) and serogroup C (MenC) capsular polysaccharides (PS)	478:595	Our laboratory has developed rapid, efficient and scalable processes for the downstream purification of N. meningitidis serogroup A (MenA) and serogroup C (MenC) capsular polysaccharides (PS).
26123432	3	1	theme	interaction	696:706	arg1	chromatography					708:721	hydrophobic interaction chromatography	684:721	hydrophobic interaction chromatography	684:721	The MenC-PS was purified with a novel 2-step procedure including de-O-acetylation and hydrophobic interaction chromatography whereas, MenA-PS was purified using a rapid method as compared to the prior art.
26123432	5	2	theme	MenC-PS	1060:1066	arg1	purification					1032:1043	the purification	1028:1043	the purification of MenA-PS and MenC-PS with reduced timelines	1028:1089	Our results provide a proof of principle for the purification of MenA-PS and MenC-PS with reduced timelines.
26123432	5	3	theme	reduced	1073:1079	arg1	timelines					1081:1089	reduced timelines	1073:1089	reduced timelines	1073:1089	Our results provide a proof of principle for the purification of MenA-PS and MenC-PS with reduced timelines.
26123432	4	4	theme	analytical	846:855	arg1	tests					857:861	various analytical tests	838:861	various analytical tests including nuclear magnetic resonance, molecular weight, composition and purity analyses	838:949	The purified PSs were analyzed by various analytical tests including nuclear magnetic resonance, molecular weight, composition and purity analyses to meet desired specifications.
26123432	4	4	theme	analytical	846:855	arg1	purity					935:940	purity	935:940	purity	935:940	The purified PSs were analyzed by various analytical tests including nuclear magnetic resonance, molecular weight, composition and purity analyses to meet desired specifications.
26123432	4	4	theme	analytical	846:855	arg1	composition					919:929	composition	919:929	composition	919:929	The purified PSs were analyzed by various analytical tests including nuclear magnetic resonance, molecular weight, composition and purity analyses to meet desired specifications.
26123432	4	4	theme	analytical	846:855	arg1	resonance					890:898	nuclear magnetic resonance	873:898	nuclear magnetic resonance	873:898	The purified PSs were analyzed by various analytical tests including nuclear magnetic resonance, molecular weight, composition and purity analyses to meet desired specifications.
26123432	4	4	theme	analytical	846:855	arg1	weight					911:916	molecular weight	901:916	molecular weight	901:916	The purified PSs were analyzed by various analytical tests including nuclear magnetic resonance, molecular weight, composition and purity analyses to meet desired specifications.
26123432	1	5	dep	out	245:247	arg1	of					249:250	of	249:250	of	249:250	The glycoconjugate vaccines against Neisseria meningitidis are highly effective, however most of these vaccines are expensive and still out of reach in the developing world as well as the technical know-how and the set-up required for the consistent production of pure polysaccharide is limited.
26123432	2	6	theme	polysaccharides	576:590	arg1	purification					493:504	the downstream purification	478:504	the downstream purification of N. meningitidis serogroup A (MenA) and serogroup C (MenC) capsular polysaccharides (PS)	478:595	Our laboratory has developed rapid, efficient and scalable processes for the downstream purification of N. meningitidis serogroup A (MenA) and serogroup C (MenC) capsular polysaccharides (PS).
26123432	2	7	theme	A	535:535	arg1	PS					593:594	PS	593:594	PS	593:594	Our laboratory has developed rapid, efficient and scalable processes for the downstream purification of N. meningitidis serogroup A (MenA) and serogroup C (MenC) capsular polysaccharides (PS).
26123432	2	7	theme	A	535:535	arg1	polysaccharides					576:590	N. meningitidis serogroup A (MenA) and serogroup C (MenC) capsular polysaccharides	509:590	N. meningitidis serogroup A (MenA) and serogroup C (MenC) capsular polysaccharides (PS)	509:595	Our laboratory has developed rapid, efficient and scalable processes for the downstream purification of N. meningitidis serogroup A (MenA) and serogroup C (MenC) capsular polysaccharides (PS).
26123432	2	8	theme	capsular	567:574	arg1	PS					593:594	PS	593:594	PS	593:594	Our laboratory has developed rapid, efficient and scalable processes for the downstream purification of N. meningitidis serogroup A (MenA) and serogroup C (MenC) capsular polysaccharides (PS).
26123432	2	8	theme	capsular	567:574	arg1	polysaccharides					576:590	N. meningitidis serogroup A (MenA) and serogroup C (MenC) capsular polysaccharides	509:590	N. meningitidis serogroup A (MenA) and serogroup C (MenC) capsular polysaccharides (PS)	509:595	Our laboratory has developed rapid, efficient and scalable processes for the downstream purification of N. meningitidis serogroup A (MenA) and serogroup C (MenC) capsular polysaccharides (PS).
26123432	5	9	with	MenA-PS	1048:1054	arg1	timelines					1081:1089	reduced timelines	1073:1089	reduced timelines	1073:1089	Our results provide a proof of principle for the purification of MenA-PS and MenC-PS with reduced timelines.
26123432	1	10	theme	developing	265:274	arg1	world					276:280	the developing world	261:280	the developing world	261:280	The glycoconjugate vaccines against Neisseria meningitidis are highly effective, however most of these vaccines are expensive and still out of reach in the developing world as well as the technical know-how and the set-up required for the consistent production of pure polysaccharide is limited.
26123432	2	11	theme	serogroup	525:533	arg1	MenA					538:541	MenA	538:541	MenA	538:541	Our laboratory has developed rapid, efficient and scalable processes for the downstream purification of N. meningitidis serogroup A (MenA) and serogroup C (MenC) capsular polysaccharides (PS).
26123432	2	11	theme	serogroup	525:533	arg1	A					535:535	N. meningitidis serogroup A	509:535	N. meningitidis serogroup A (MenA)	509:542	Our laboratory has developed rapid, efficient and scalable processes for the downstream purification of N. meningitidis serogroup A (MenA) and serogroup C (MenC) capsular polysaccharides (PS).
26123432	1	12	theme	consistent	348:357	arg1	production					359:368	the consistent production	344:368	the consistent production of pure polysaccharide	344:391	The glycoconjugate vaccines against Neisseria meningitidis are highly effective, however most of these vaccines are expensive and still out of reach in the developing world as well as the technical know-how and the set-up required for the consistent production of pure polysaccharide is limited.
26123432	2	13	theme	N. meningitidis	509:523	arg1	MenA					538:541	MenA	538:541	MenA	538:541	Our laboratory has developed rapid, efficient and scalable processes for the downstream purification of N. meningitidis serogroup A (MenA) and serogroup C (MenC) capsular polysaccharides (PS).
26123432	2	13	theme	N. meningitidis	509:523	arg1	A					535:535	N. meningitidis serogroup A	509:535	N. meningitidis serogroup A (MenA)	509:542	Our laboratory has developed rapid, efficient and scalable processes for the downstream purification of N. meningitidis serogroup A (MenA) and serogroup C (MenC) capsular polysaccharides (PS).
26123432	2	14	theme	efficient	441:449	arg1	processes					464:472	rapid, efficient and scalable processes	434:472	rapid, efficient and scalable processes	434:472	Our laboratory has developed rapid, efficient and scalable processes for the downstream purification of N. meningitidis serogroup A (MenA) and serogroup C (MenC) capsular polysaccharides (PS).
26123432	4	15	theme	various	838:844	arg1	tests					857:861	various analytical tests	838:861	various analytical tests including nuclear magnetic resonance, molecular weight, composition and purity analyses	838:949	The purified PSs were analyzed by various analytical tests including nuclear magnetic resonance, molecular weight, composition and purity analyses to meet desired specifications.
26123432	4	15	theme	various	838:844	arg1	purity					935:940	purity	935:940	purity	935:940	The purified PSs were analyzed by various analytical tests including nuclear magnetic resonance, molecular weight, composition and purity analyses to meet desired specifications.
26123432	4	15	theme	various	838:844	arg1	composition					919:929	composition	919:929	composition	919:929	The purified PSs were analyzed by various analytical tests including nuclear magnetic resonance, molecular weight, composition and purity analyses to meet desired specifications.
26123432	4	15	theme	various	838:844	arg1	resonance					890:898	nuclear magnetic resonance	873:898	nuclear magnetic resonance	873:898	The purified PSs were analyzed by various analytical tests including nuclear magnetic resonance, molecular weight, composition and purity analyses to meet desired specifications.
26123432	4	15	theme	various	838:844	arg1	weight					911:916	molecular weight	901:916	molecular weight	901:916	The purified PSs were analyzed by various analytical tests including nuclear magnetic resonance, molecular weight, composition and purity analyses to meet desired specifications.
26123432	3	16	dep	method	767:772	arg1	compared					777:784	compared	777:784	compared to the prior art	777:801	The MenC-PS was purified with a novel 2-step procedure including de-O-acetylation and hydrophobic interaction chromatography whereas, MenA-PS was purified using a rapid method as compared to the prior art.
26123432	0	17	theme	Rapid	0:4	arg1	processes					6:14	Rapid processes	0:14	Rapid processes for purification of capsular polysaccharides from Neisseria meningitidis	0:87	Rapid processes for purification of capsular polysaccharides from Neisseria meningitidis serogroups A and C.
26123432	1	18	dep	effective	179:187	arg1	effective					179:187	effective	179:187	effective	179:187	The glycoconjugate vaccines against Neisseria meningitidis are highly effective, however most of these vaccines are expensive and still out of reach in the developing world as well as the technical know-how and the set-up required for the consistent production of pure polysaccharide is limited.
26123432	1	18	dep	effective	179:187	arg1	vaccines					128:135	The glycoconjugate vaccines	109:135	The glycoconjugate vaccines against Neisseria meningitidis	109:166	The glycoconjugate vaccines against Neisseria meningitidis are highly effective, however most of these vaccines are expensive and still out of reach in the developing world as well as the technical know-how and the set-up required for the consistent production of pure polysaccharide is limited.
26123432	1	18	dep	effective	179:187	arg1	reach					252:256	reach	252:256	still out of reach in the developing world as well as the technical know-how	239:314	The glycoconjugate vaccines against Neisseria meningitidis are highly effective, however most of these vaccines are expensive and still out of reach in the developing world as well as the technical know-how and the set-up required for the consistent production of pure polysaccharide is limited.
26123432	1	18	dep	effective	179:187	arg1	expensive					225:233	expensive	225:233	expensive	225:233	The glycoconjugate vaccines against Neisseria meningitidis are highly effective, however most of these vaccines are expensive and still out of reach in the developing world as well as the technical know-how and the set-up required for the consistent production of pure polysaccharide is limited.
26123432	2	19	theme	rapid	434:438	arg1	processes					464:472	rapid, efficient and scalable processes	434:472	rapid, efficient and scalable processes	434:472	Our laboratory has developed rapid, efficient and scalable processes for the downstream purification of N. meningitidis serogroup A (MenA) and serogroup C (MenC) capsular polysaccharides (PS).
26123432	2	20	theme	serogroup	548:556	arg1	MenC					561:564	MenC	561:564	MenC	561:564	Our laboratory has developed rapid, efficient and scalable processes for the downstream purification of N. meningitidis serogroup A (MenA) and serogroup C (MenC) capsular polysaccharides (PS).
26123432	2	20	theme	serogroup	548:556	arg1	C					558:558	serogroup C	548:558	serogroup C (MenC)	548:565	Our laboratory has developed rapid, efficient and scalable processes for the downstream purification of N. meningitidis serogroup A (MenA) and serogroup C (MenC) capsular polysaccharides (PS).
26123432	1	21	theme	pure	373:376	arg1	polysaccharide					378:391	pure polysaccharide	373:391	pure polysaccharide	373:391	The glycoconjugate vaccines against Neisseria meningitidis are highly effective, however most of these vaccines are expensive and still out of reach in the developing world as well as the technical know-how and the set-up required for the consistent production of pure polysaccharide is limited.
26123432	4	22	dep	resonance	890:898	arg1	analyses					942:949	analyses	942:949	analyses	942:949	The purified PSs were analyzed by various analytical tests including nuclear magnetic resonance, molecular weight, composition and purity analyses to meet desired specifications.
26123432	5	23	with	MenC-PS	1060:1066	arg1	timelines					1081:1089	reduced timelines	1073:1089	reduced timelines	1073:1089	Our results provide a proof of principle for the purification of MenA-PS and MenC-PS with reduced timelines.
26123432	0	24	theme	capsular	36:43	arg1	polysaccharides					45:59	capsular polysaccharides	36:59	capsular polysaccharides from Neisseria meningitidis	36:87	Rapid processes for purification of capsular polysaccharides from Neisseria meningitidis serogroups A and C.
26123432	1	25	theme	polysaccharide	378:391	arg1	production					359:368	the consistent production	344:368	the consistent production of pure polysaccharide	344:391	The glycoconjugate vaccines against Neisseria meningitidis are highly effective, however most of these vaccines are expensive and still out of reach in the developing world as well as the technical know-how and the set-up required for the consistent production of pure polysaccharide is limited.
26123432	0	26	from	meningitidis	76:87	arg1	purification					20:31	purification	20:31	purification of capsular polysaccharides from Neisseria meningitidis	20:87	Rapid processes for purification of capsular polysaccharides from Neisseria meningitidis serogroups A and C.
26123432	0	26	from	meningitidis	76:87	arg1	polysaccharides					45:59	capsular polysaccharides	36:59	capsular polysaccharides from Neisseria meningitidis	36:87	Rapid processes for purification of capsular polysaccharides from Neisseria meningitidis serogroups A and C.
26123432	5	27	theme	principle	1014:1022	arg1	proof					1005:1009	a proof	1003:1009	a proof of principle for the purification of MenA-PS and MenC-PS with reduced timelines	1003:1089	Our results provide a proof of principle for the purification of MenA-PS and MenC-PS with reduced timelines.
26123432	2	28	theme	scalable	455:462	arg1	processes					464:472	rapid, efficient and scalable processes	434:472	rapid, efficient and scalable processes	434:472	Our laboratory has developed rapid, efficient and scalable processes for the downstream purification of N. meningitidis serogroup A (MenA) and serogroup C (MenC) capsular polysaccharides (PS).
26123432	1	29	theme	technical	297:305	arg1	know-how					307:314	the technical know-how	293:314	the technical know-how	293:314	The glycoconjugate vaccines against Neisseria meningitidis are highly effective, however most of these vaccines are expensive and still out of reach in the developing world as well as the technical know-how and the set-up required for the consistent production of pure polysaccharide is limited.
26123432	4	30	theme	desired	959:965	arg1	specifications					967:980	desired specifications	959:980	desired specifications	959:980	The purified PSs were analyzed by various analytical tests including nuclear magnetic resonance, molecular weight, composition and purity analyses to meet desired specifications.
26123432	1	31	theme	glycoconjugate	113:126	arg1	effective					179:187	effective	179:187	effective	179:187	The glycoconjugate vaccines against Neisseria meningitidis are highly effective, however most of these vaccines are expensive and still out of reach in the developing world as well as the technical know-how and the set-up required for the consistent production of pure polysaccharide is limited.
26123432	1	31	theme	glycoconjugate	113:126	arg1	vaccines					128:135	The glycoconjugate vaccines	109:135	The glycoconjugate vaccines against Neisseria meningitidis	109:166	The glycoconjugate vaccines against Neisseria meningitidis are highly effective, however most of these vaccines are expensive and still out of reach in the developing world as well as the technical know-how and the set-up required for the consistent production of pure polysaccharide is limited.
26123432	1	31	theme	glycoconjugate	113:126	arg1	reach					252:256	reach	252:256	still out of reach in the developing world as well as the technical know-how	239:314	The glycoconjugate vaccines against Neisseria meningitidis are highly effective, however most of these vaccines are expensive and still out of reach in the developing world as well as the technical know-how and the set-up required for the consistent production of pure polysaccharide is limited.
26123432	1	31	theme	glycoconjugate	113:126	arg1	expensive					225:233	expensive	225:233	expensive	225:233	The glycoconjugate vaccines against Neisseria meningitidis are highly effective, however most of these vaccines are expensive and still out of reach in the developing world as well as the technical know-how and the set-up required for the consistent production of pure polysaccharide is limited.
26123432	0	32	theme	polysaccharides	45:59	arg1	purification					20:31	purification	20:31	purification of capsular polysaccharides from Neisseria meningitidis	20:87	Rapid processes for purification of capsular polysaccharides from Neisseria meningitidis serogroups A and C.
26123432	3	33	theme	rapid	761:765	arg1	method					767:772	a rapid method	759:772	a rapid method as compared to the prior art	759:801	The MenC-PS was purified with a novel 2-step procedure including de-O-acetylation and hydrophobic interaction chromatography whereas, MenA-PS was purified using a rapid method as compared to the prior art.
26123432	3	34	theme	hydrophobic	684:694	arg1	chromatography					708:721	hydrophobic interaction chromatography	684:721	hydrophobic interaction chromatography	684:721	The MenC-PS was purified with a novel 2-step procedure including de-O-acetylation and hydrophobic interaction chromatography whereas, MenA-PS was purified using a rapid method as compared to the prior art.
26123432	4	35	theme	purified	808:815	arg1	PSs					817:819	The purified PSs	804:819	The purified PSs	804:819	The purified PSs were analyzed by various analytical tests including nuclear magnetic resonance, molecular weight, composition and purity analyses to meet desired specifications.
26123432	4	36	theme	nuclear	873:879	arg1	resonance					890:898	nuclear magnetic resonance	873:898	nuclear magnetic resonance	873:898	The purified PSs were analyzed by various analytical tests including nuclear magnetic resonance, molecular weight, composition and purity analyses to meet desired specifications.
26123432	4	37	theme	molecular	901:909	arg1	weight					911:916	molecular weight	901:916	molecular weight	901:916	The purified PSs were analyzed by various analytical tests including nuclear magnetic resonance, molecular weight, composition and purity analyses to meet desired specifications.
26123432	3	38	theme	prior	793:797	arg1	art					799:801	the prior art	789:801	the prior art	789:801	The MenC-PS was purified with a novel 2-step procedure including de-O-acetylation and hydrophobic interaction chromatography whereas, MenA-PS was purified using a rapid method as compared to the prior art.
26123432	2	39	theme	C	558:558	arg1	PS					593:594	PS	593:594	PS	593:594	Our laboratory has developed rapid, efficient and scalable processes for the downstream purification of N. meningitidis serogroup A (MenA) and serogroup C (MenC) capsular polysaccharides (PS).
26123432	2	39	theme	C	558:558	arg1	polysaccharides					576:590	N. meningitidis serogroup A (MenA) and serogroup C (MenC) capsular polysaccharides	509:590	N. meningitidis serogroup A (MenA) and serogroup C (MenC) capsular polysaccharides (PS)	509:595	Our laboratory has developed rapid, efficient and scalable processes for the downstream purification of N. meningitidis serogroup A (MenA) and serogroup C (MenC) capsular polysaccharides (PS).
26123432	3	40	theme	novel	630:634	arg1	procedure					643:651	a novel 2-step procedure	628:651	a novel 2-step procedure including de-O-acetylation and hydrophobic interaction chromatography	628:721	The MenC-PS was purified with a novel 2-step procedure including de-O-acetylation and hydrophobic interaction chromatography whereas, MenA-PS was purified using a rapid method as compared to the prior art.
26123432	1	41	theme	Neisseria	145:153	arg1	meningitidis					155:166	Neisseria meningitidis	145:166	Neisseria meningitidis	145:166	The glycoconjugate vaccines against Neisseria meningitidis are highly effective, however most of these vaccines are expensive and still out of reach in the developing world as well as the technical know-how and the set-up required for the consistent production of pure polysaccharide is limited.
26123432	5	42	theme	MenA-PS	1048:1054	arg1	purification					1032:1043	the purification	1028:1043	the purification of MenA-PS and MenC-PS with reduced timelines	1028:1089	Our results provide a proof of principle for the purification of MenA-PS and MenC-PS with reduced timelines.
26123432	3	43	theme	2-step	636:641	arg1	procedure					643:651	a novel 2-step procedure	628:651	a novel 2-step procedure including de-O-acetylation and hydrophobic interaction chromatography	628:721	The MenC-PS was purified with a novel 2-step procedure including de-O-acetylation and hydrophobic interaction chromatography whereas, MenA-PS was purified using a rapid method as compared to the prior art.
26123432	4	44	theme	magnetic	881:888	arg1	resonance					890:898	nuclear magnetic resonance	873:898	nuclear magnetic resonance	873:898	The purified PSs were analyzed by various analytical tests including nuclear magnetic resonance, molecular weight, composition and purity analyses to meet desired specifications.
26123432	0	45	theme	Neisseria	66:74	arg1	meningitidis					76:87	Neisseria meningitidis	66:87	Neisseria meningitidis	66:87	Rapid processes for purification of capsular polysaccharides from Neisseria meningitidis serogroups A and C.
26123432	0	46	from	purification	20:31	arg1	meningitidis					76:87	Neisseria meningitidis	66:87	Neisseria meningitidis	66:87	Rapid processes for purification of capsular polysaccharides from Neisseria meningitidis serogroups A and C.
29279125	0	0	theme	barrier	80:86	arg1	properties					88:97	the mechanical and water vapor barrier properties	49:97	properties	88:97	Effect of polyethyleneimine modified graphene on the mechanical and water vapor barrier properties of methyl cellulose composite films.
29279125	4	1	theme	enhanced	716:723	arg1	hydrophobicity					733:746	an enhanced surface hydrophobicity	713:746	an enhanced surface hydrophobicity	713:746	The addition of PEI-RGO resulted in an enhanced surface hydrophobicity and a tortuous diffusion pathway for water molecules.
29279125	5	2	theme	vapor	808:812	arg1	permeability					814:825	Water vapor permeability	802:825	Water vapor permeability of PEI-RGO/MC with loading of 3.0% of surface modified graphene	802:889	Water vapor permeability of PEI-RGO/MC with loading of 3.0% of surface modified graphene was as low as 5.98×10-11gmm-2s-1Pa-1.
29279125	1	3	theme	novel	148:152	arg1	films					186:190	novel methyl cellulose (MC) composite films	148:190	novel methyl cellulose (MC) composite films	148:190	A series of novel methyl cellulose (MC) composite films were prepared using polyethyleneimine reduced graphene oxide (PEI-RGO) as an effective filler for water vapor barrier application.
29279125	6	4	theme	barrier	1063:1069	arg1	performance					1071:1081	the improved water vapor barrier performance	1038:1081	the improved water vapor barrier performance of the PEI-RGO/MC composite films	1038:1115	The synergistic effects of enhanced surface hydrophobicity and tortuous diffusion pathway were accounted for the improved water vapor barrier performance of the PEI-RGO/MC composite films.
29279125	0	5	theme	vapor	74:78	arg1	properties					88:97	the mechanical and water vapor barrier properties	49:97	properties	88:97	Effect of polyethyleneimine modified graphene on the mechanical and water vapor barrier properties of methyl cellulose composite films.
29279125	6	6	theme	enhanced	956:963	arg1	hydrophobicity					973:986	enhanced surface hydrophobicity	956:986	enhanced surface hydrophobicity	956:986	The synergistic effects of enhanced surface hydrophobicity and tortuous diffusion pathway were accounted for the improved water vapor barrier performance of the PEI-RGO/MC composite films.
29279125	1	7	theme	methyl	154:159	arg1	films					186:190	novel methyl cellulose (MC) composite films	148:190	novel methyl cellulose (MC) composite films	148:190	A series of novel methyl cellulose (MC) composite films were prepared using polyethyleneimine reduced graphene oxide (PEI-RGO) as an effective filler for water vapor barrier application.
29279125	6	8	theme	vapor	1057:1061	arg1	performance					1071:1081	the improved water vapor barrier performance	1038:1081	the improved water vapor barrier performance of the PEI-RGO/MC composite films	1038:1115	The synergistic effects of enhanced surface hydrophobicity and tortuous diffusion pathway were accounted for the improved water vapor barrier performance of the PEI-RGO/MC composite films.
29279125	4	9	theme	water	785:789	arg1	molecules					791:799	water molecules	785:799	water molecules	785:799	The addition of PEI-RGO resulted in an enhanced surface hydrophobicity and a tortuous diffusion pathway for water molecules.
29279125	6	10	theme	water	1051:1055	arg1	performance					1071:1081	the improved water vapor barrier performance	1038:1081	the improved water vapor barrier performance of the PEI-RGO/MC composite films	1038:1115	The synergistic effects of enhanced surface hydrophobicity and tortuous diffusion pathway were accounted for the improved water vapor barrier performance of the PEI-RGO/MC composite films.
29279125	3	11	theme	experimental	522:533	arg1	results					551:557	The experimental and theoretical results	518:557	The experimental and theoretical results	518:557	The experimental and theoretical results exhibited that PEI-RGO was uniformly dispersed in the MC matrix without aggregation and formed an aligned dispersion.
29279125	6	12	theme	improved	1042:1049	arg1	performance					1071:1081	the improved water vapor barrier performance	1038:1081	the improved water vapor barrier performance of the PEI-RGO/MC composite films	1038:1115	The synergistic effects of enhanced surface hydrophobicity and tortuous diffusion pathway were accounted for the improved water vapor barrier performance of the PEI-RGO/MC composite films.
29279125	0	13	theme	cellulose	109:117	arg1	films					129:133	methyl cellulose composite films	102:133	methyl cellulose composite films	102:133	Effect of polyethyleneimine modified graphene on the mechanical and water vapor barrier properties of methyl cellulose composite films.
29279125	6	14	theme	synergistic	933:943	arg1	effects					945:951	The synergistic effects	929:951	The synergistic effects of enhanced surface hydrophobicity and tortuous diffusion pathway	929:1017	The synergistic effects of enhanced surface hydrophobicity and tortuous diffusion pathway were accounted for the improved water vapor barrier performance of the PEI-RGO/MC composite films.
29279125	5	15	theme	modified	873:880	arg1	graphene					882:889	surface modified graphene	865:889	surface modified graphene	865:889	Water vapor permeability of PEI-RGO/MC with loading of 3.0% of surface modified graphene was as low as 5.98×10-11gmm-2s-1Pa-1.
29279125	2	16	theme	X-ray	424:428	arg1	spectroscopy					410:421	spectroscopy	410:421	spectroscopy	410:421	The as-prepared PEI-RGO/MC composites were characterized by Fourier transform infrared spectroscopy, X-ray diffraction, thermogravimetric analysis, tensile test and scanning electron microscopy.
29279125	2	16	theme	X-ray	424:428	arg1	diffraction					430:440	X-ray diffraction	424:440	X-ray diffraction	424:440	The as-prepared PEI-RGO/MC composites were characterized by Fourier transform infrared spectroscopy, X-ray diffraction, thermogravimetric analysis, tensile test and scanning electron microscopy.
29279125	1	17	theme	cellulose	161:169	arg1	films					186:190	novel methyl cellulose (MC) composite films	148:190	novel methyl cellulose (MC) composite films	148:190	A series of novel methyl cellulose (MC) composite films were prepared using polyethyleneimine reduced graphene oxide (PEI-RGO) as an effective filler for water vapor barrier application.
29279125	0	18	theme	methyl	102:107	arg1	films					129:133	methyl cellulose composite films	102:133	methyl cellulose composite films	102:133	Effect of polyethyleneimine modified graphene on the mechanical and water vapor barrier properties of methyl cellulose composite films.
29279125	2	19	theme	electron	497:504	arg1	microscopy					506:515	scanning electron microscopy	488:515	scanning electron microscopy	488:515	The as-prepared PEI-RGO/MC composites were characterized by Fourier transform infrared spectroscopy, X-ray diffraction, thermogravimetric analysis, tensile test and scanning electron microscopy.
29279125	2	19	theme	electron	497:504	arg1	spectroscopy					410:421	spectroscopy	410:421	spectroscopy	410:421	The as-prepared PEI-RGO/MC composites were characterized by Fourier transform infrared spectroscopy, X-ray diffraction, thermogravimetric analysis, tensile test and scanning electron microscopy.
29279125	5	20	with	PEI-RGO/MC	830:839	arg1	loading					846:852	loading	846:852	loading of 3.0% of surface modified graphene	846:889	Water vapor permeability of PEI-RGO/MC with loading of 3.0% of surface modified graphene was as low as 5.98×10-11gmm-2s-1Pa-1.
29279125	6	21	theme	tortuous	992:999	arg1	pathway					1011:1017	tortuous diffusion pathway	992:1017	tortuous diffusion pathway	992:1017	The synergistic effects of enhanced surface hydrophobicity and tortuous diffusion pathway were accounted for the improved water vapor barrier performance of the PEI-RGO/MC composite films.
29279125	0	22	from	graphene	37:44	arg1	properties					88:97	the mechanical and water vapor barrier properties	49:97	properties	88:97	Effect of polyethyleneimine modified graphene on the mechanical and water vapor barrier properties of methyl cellulose composite films.
29279125	0	22	from	graphene	37:44	arg1	mechanical					53:62	mechanical	53:62	mechanical	53:62	Effect of polyethyleneimine modified graphene on the mechanical and water vapor barrier properties of methyl cellulose composite films.
29279125	1	23	theme	MC	172:173	arg1	films					186:190	novel methyl cellulose (MC) composite films	148:190	novel methyl cellulose (MC) composite films	148:190	A series of novel methyl cellulose (MC) composite films were prepared using polyethyleneimine reduced graphene oxide (PEI-RGO) as an effective filler for water vapor barrier application.
29279125	4	24	theme	surface	725:731	arg1	hydrophobicity					733:746	an enhanced surface hydrophobicity	713:746	an enhanced surface hydrophobicity	713:746	The addition of PEI-RGO resulted in an enhanced surface hydrophobicity and a tortuous diffusion pathway for water molecules.
29279125	2	25	theme	thermogravimetric	443:459	arg1	analysis					461:468	thermogravimetric analysis	443:468	thermogravimetric analysis	443:468	The as-prepared PEI-RGO/MC composites were characterized by Fourier transform infrared spectroscopy, X-ray diffraction, thermogravimetric analysis, tensile test and scanning electron microscopy.
29279125	2	25	theme	thermogravimetric	443:459	arg1	spectroscopy					410:421	spectroscopy	410:421	spectroscopy	410:421	The as-prepared PEI-RGO/MC composites were characterized by Fourier transform infrared spectroscopy, X-ray diffraction, thermogravimetric analysis, tensile test and scanning electron microscopy.
29279125	0	26	theme	films	129:133	arg1	mechanical					53:62	mechanical	53:62	mechanical	53:62	Effect of polyethyleneimine modified graphene on the mechanical and water vapor barrier properties of methyl cellulose composite films.
29279125	1	27	theme	composite	176:184	arg1	films					186:190	novel methyl cellulose (MC) composite films	148:190	novel methyl cellulose (MC) composite films	148:190	A series of novel methyl cellulose (MC) composite films were prepared using polyethyleneimine reduced graphene oxide (PEI-RGO) as an effective filler for water vapor barrier application.
29279125	0	28	theme	polyethyleneimine	10:26	arg1	Effect					0:5	Effect	0:5	Effect of polyethyleneimine	0:26	Effect of polyethyleneimine modified graphene on the mechanical and water vapor barrier properties of methyl cellulose composite films.
29279125	1	29	theme	effective	269:277	arg1	filler					279:284	an effective filler	266:284	an effective filler for water vapor barrier application	266:320	A series of novel methyl cellulose (MC) composite films were prepared using polyethyleneimine reduced graphene oxide (PEI-RGO) as an effective filler for water vapor barrier application.
29279125	1	29	theme	effective	269:277	arg1	polyethyleneimine					212:228	polyethyleneimine reduced graphene oxide (PEI-RGO)	212:261	polyethyleneimine reduced graphene oxide (PEI-RGO)	212:261	A series of novel methyl cellulose (MC) composite films were prepared using polyethyleneimine reduced graphene oxide (PEI-RGO) as an effective filler for water vapor barrier application.
29279125	0	30	theme	composite	119:127	arg1	films					129:133	methyl cellulose composite films	102:133	methyl cellulose composite films	102:133	Effect of polyethyleneimine modified graphene on the mechanical and water vapor barrier properties of methyl cellulose composite films.
29279125	2	31	theme	PEI-RGO/MC	339:348	arg1	composites					350:359	The as-prepared PEI-RGO/MC composites	323:359	The as-prepared PEI-RGO/MC composites	323:359	The as-prepared PEI-RGO/MC composites were characterized by Fourier transform infrared spectroscopy, X-ray diffraction, thermogravimetric analysis, tensile test and scanning electron microscopy.
29279125	6	32	theme	surface	965:971	arg1	hydrophobicity					973:986	enhanced surface hydrophobicity	956:986	enhanced surface hydrophobicity	956:986	The synergistic effects of enhanced surface hydrophobicity and tortuous diffusion pathway were accounted for the improved water vapor barrier performance of the PEI-RGO/MC composite films.
29279125	1	33	theme	films	186:190	arg1	series					138:143	A series	136:143	A series of novel methyl cellulose (MC) composite films	136:190	A series of novel methyl cellulose (MC) composite films were prepared using polyethyleneimine reduced graphene oxide (PEI-RGO) as an effective filler for water vapor barrier application.
29279125	2	34	theme	scanning	488:495	arg1	microscopy					506:515	scanning electron microscopy	488:515	scanning electron microscopy	488:515	The as-prepared PEI-RGO/MC composites were characterized by Fourier transform infrared spectroscopy, X-ray diffraction, thermogravimetric analysis, tensile test and scanning electron microscopy.
29279125	2	34	theme	scanning	488:495	arg1	spectroscopy					410:421	spectroscopy	410:421	spectroscopy	410:421	The as-prepared PEI-RGO/MC composites were characterized by Fourier transform infrared spectroscopy, X-ray diffraction, thermogravimetric analysis, tensile test and scanning electron microscopy.
29279125	6	35	theme	films	1111:1115	arg1	performance					1071:1081	the improved water vapor barrier performance	1038:1081	the improved water vapor barrier performance of the PEI-RGO/MC composite films	1038:1115	The synergistic effects of enhanced surface hydrophobicity and tortuous diffusion pathway were accounted for the improved water vapor barrier performance of the PEI-RGO/MC composite films.
29279125	5	36	theme	graphene	882:889	arg1	graphene					882:889	surface modified graphene	865:889	surface modified graphene	865:889	Water vapor permeability of PEI-RGO/MC with loading of 3.0% of surface modified graphene was as low as 5.98×10-11gmm-2s-1Pa-1.
29279125	5	36	theme	graphene	882:889	arg1	%					860:860	3.0%	857:860	3.0% of surface modified graphene	857:889	Water vapor permeability of PEI-RGO/MC with loading of 3.0% of surface modified graphene was as low as 5.98×10-11gmm-2s-1Pa-1.
29279125	4	37	theme	PEI-RGO	693:699	arg1	addition					681:688	The addition	677:688	The addition of PEI-RGO	677:699	The addition of PEI-RGO resulted in an enhanced surface hydrophobicity and a tortuous diffusion pathway for water molecules.
29279125	6	38	theme	pathway	1011:1017	arg1	effects					945:951	The synergistic effects	929:951	The synergistic effects of enhanced surface hydrophobicity and tortuous diffusion pathway	929:1017	The synergistic effects of enhanced surface hydrophobicity and tortuous diffusion pathway were accounted for the improved water vapor barrier performance of the PEI-RGO/MC composite films.
29279125	5	39	theme	PEI-RGO/MC	830:839	arg1	permeability					814:825	Water vapor permeability	802:825	Water vapor permeability of PEI-RGO/MC with loading of 3.0% of surface modified graphene	802:889	Water vapor permeability of PEI-RGO/MC with loading of 3.0% of surface modified graphene was as low as 5.98×10-11gmm-2s-1Pa-1.
29279125	5	40	theme	%	860:860	arg1	loading					846:852	loading	846:852	loading of 3.0% of surface modified graphene	846:889	Water vapor permeability of PEI-RGO/MC with loading of 3.0% of surface modified graphene was as low as 5.98×10-11gmm-2s-1Pa-1.
29279125	6	41	theme	composite	1101:1109	arg1	films					1111:1115	the PEI-RGO/MC composite films	1086:1115	the PEI-RGO/MC composite films	1086:1115	The synergistic effects of enhanced surface hydrophobicity and tortuous diffusion pathway were accounted for the improved water vapor barrier performance of the PEI-RGO/MC composite films.
29279125	1	42	theme	water	290:294	arg1	application					310:320	water vapor barrier application	290:320	water vapor barrier application	290:320	A series of novel methyl cellulose (MC) composite films were prepared using polyethyleneimine reduced graphene oxide (PEI-RGO) as an effective filler for water vapor barrier application.
29279125	1	43	dep	polyethyleneimine	212:228	arg1	PEI-RGO					254:260	PEI-RGO	254:260	PEI-RGO	254:260	A series of novel methyl cellulose (MC) composite films were prepared using polyethyleneimine reduced graphene oxide (PEI-RGO) as an effective filler for water vapor barrier application.
29279125	1	43	dep	polyethyleneimine	212:228	arg1	oxide					247:251	reduced graphene oxide	230:251	polyethyleneimine reduced graphene oxide (PEI-RGO)	212:261	A series of novel methyl cellulose (MC) composite films were prepared using polyethyleneimine reduced graphene oxide (PEI-RGO) as an effective filler for water vapor barrier application.
29279125	3	44	theme	MC	613:614	arg1	matrix					616:621	the MC matrix	609:621	the MC matrix	609:621	The experimental and theoretical results exhibited that PEI-RGO was uniformly dispersed in the MC matrix without aggregation and formed an aligned dispersion.
29279125	2	45	theme	as-prepared	327:337	arg1	composites					350:359	The as-prepared PEI-RGO/MC composites	323:359	The as-prepared PEI-RGO/MC composites	323:359	The as-prepared PEI-RGO/MC composites were characterized by Fourier transform infrared spectroscopy, X-ray diffraction, thermogravimetric analysis, tensile test and scanning electron microscopy.
29279125	2	46	theme	tensile	471:477	arg1	test					479:482	tensile test	471:482	tensile test	471:482	The as-prepared PEI-RGO/MC composites were characterized by Fourier transform infrared spectroscopy, X-ray diffraction, thermogravimetric analysis, tensile test and scanning electron microscopy.
29279125	2	46	theme	tensile	471:477	arg1	spectroscopy					410:421	spectroscopy	410:421	spectroscopy	410:421	The as-prepared PEI-RGO/MC composites were characterized by Fourier transform infrared spectroscopy, X-ray diffraction, thermogravimetric analysis, tensile test and scanning electron microscopy.
29279125	1	47	theme	vapor	296:300	arg1	application					310:320	water vapor barrier application	290:320	water vapor barrier application	290:320	A series of novel methyl cellulose (MC) composite films were prepared using polyethyleneimine reduced graphene oxide (PEI-RGO) as an effective filler for water vapor barrier application.
29279125	1	48	theme	barrier	302:308	arg1	application					310:320	water vapor barrier application	290:320	water vapor barrier application	290:320	A series of novel methyl cellulose (MC) composite films were prepared using polyethyleneimine reduced graphene oxide (PEI-RGO) as an effective filler for water vapor barrier application.
29279125	2	49	dep	Fourier	383:389	arg1	transform					391:399	transform	391:399	transform infrared spectroscopy, X-ray diffraction, thermogravimetric analysis, tensile test and scanning electron microscopy	391:515	The as-prepared PEI-RGO/MC composites were characterized by Fourier transform infrared spectroscopy, X-ray diffraction, thermogravimetric analysis, tensile test and scanning electron microscopy.
29279125	4	50	theme	diffusion	763:771	arg1	pathway					773:779	a tortuous diffusion pathway	752:779	a tortuous diffusion pathway for water molecules	752:799	The addition of PEI-RGO resulted in an enhanced surface hydrophobicity and a tortuous diffusion pathway for water molecules.
29279125	6	51	theme	PEI-RGO/MC	1090:1099	arg1	films					1111:1115	the PEI-RGO/MC composite films	1086:1115	the PEI-RGO/MC composite films	1086:1115	The synergistic effects of enhanced surface hydrophobicity and tortuous diffusion pathway were accounted for the improved water vapor barrier performance of the PEI-RGO/MC composite films.
29279125	3	52	theme	aligned	657:663	arg1	dispersion					665:674	an aligned dispersion	654:674	an aligned dispersion	654:674	The experimental and theoretical results exhibited that PEI-RGO was uniformly dispersed in the MC matrix without aggregation and formed an aligned dispersion.
29279125	4	53	theme	tortuous	754:761	arg1	pathway					773:779	a tortuous diffusion pathway	752:779	a tortuous diffusion pathway for water molecules	752:799	The addition of PEI-RGO resulted in an enhanced surface hydrophobicity and a tortuous diffusion pathway for water molecules.
29279125	0	54	theme	water	68:72	arg1	properties					88:97	the mechanical and water vapor barrier properties	49:97	properties	88:97	Effect of polyethyleneimine modified graphene on the mechanical and water vapor barrier properties of methyl cellulose composite films.
29279125	5	55	theme	surface	865:871	arg1	graphene					882:889	surface modified graphene	865:889	surface modified graphene	865:889	Water vapor permeability of PEI-RGO/MC with loading of 3.0% of surface modified graphene was as low as 5.98×10-11gmm-2s-1Pa-1.
29279125	3	56	theme	theoretical	539:549	arg1	results					551:557	The experimental and theoretical results	518:557	The experimental and theoretical results	518:557	The experimental and theoretical results exhibited that PEI-RGO was uniformly dispersed in the MC matrix without aggregation and formed an aligned dispersion.
29279125	1	57	theme	reduced	230:236	arg1	PEI-RGO					254:260	PEI-RGO	254:260	PEI-RGO	254:260	A series of novel methyl cellulose (MC) composite films were prepared using polyethyleneimine reduced graphene oxide (PEI-RGO) as an effective filler for water vapor barrier application.
29279125	1	57	theme	reduced	230:236	arg1	oxide					247:251	reduced graphene oxide	230:251	polyethyleneimine reduced graphene oxide (PEI-RGO)	212:261	A series of novel methyl cellulose (MC) composite films were prepared using polyethyleneimine reduced graphene oxide (PEI-RGO) as an effective filler for water vapor barrier application.
29279125	5	58	theme	Water	802:806	arg1	permeability					814:825	Water vapor permeability	802:825	Water vapor permeability of PEI-RGO/MC with loading of 3.0% of surface modified graphene	802:889	Water vapor permeability of PEI-RGO/MC with loading of 3.0% of surface modified graphene was as low as 5.98×10-11gmm-2s-1Pa-1.
29279125	6	59	theme	hydrophobicity	973:986	arg1	effects					945:951	The synergistic effects	929:951	The synergistic effects of enhanced surface hydrophobicity and tortuous diffusion pathway	929:1017	The synergistic effects of enhanced surface hydrophobicity and tortuous diffusion pathway were accounted for the improved water vapor barrier performance of the PEI-RGO/MC composite films.
29279125	2	60	dep	transform	391:399	arg1	infrared					401:408	infrared	401:408	transform infrared spectroscopy, X-ray diffraction, thermogravimetric analysis, tensile test and scanning electron microscopy	391:515	The as-prepared PEI-RGO/MC composites were characterized by Fourier transform infrared spectroscopy, X-ray diffraction, thermogravimetric analysis, tensile test and scanning electron microscopy.
29279125	6	61	theme	diffusion	1001:1009	arg1	pathway					1011:1017	tortuous diffusion pathway	992:1017	tortuous diffusion pathway	992:1017	The synergistic effects of enhanced surface hydrophobicity and tortuous diffusion pathway were accounted for the improved water vapor barrier performance of the PEI-RGO/MC composite films.
29279125	1	62	theme	graphene	238:245	arg1	PEI-RGO					254:260	PEI-RGO	254:260	PEI-RGO	254:260	A series of novel methyl cellulose (MC) composite films were prepared using polyethyleneimine reduced graphene oxide (PEI-RGO) as an effective filler for water vapor barrier application.
29279125	1	62	theme	graphene	238:245	arg1	oxide					247:251	reduced graphene oxide	230:251	polyethyleneimine reduced graphene oxide (PEI-RGO)	212:261	A series of novel methyl cellulose (MC) composite films were prepared using polyethyleneimine reduced graphene oxide (PEI-RGO) as an effective filler for water vapor barrier application.
27295282	9	0	used	used	1592:1595	arg2	HS					1586:1587	commercial HS	1575:1587	commercial HS	1575:1587	Great care has to be taken in drawing conclusions from investigations of structural features of HS and specificities of HS interaction with proteins when commercial HS is used without further analysis.
27295282	6	1	theme	porcine	1120:1126	arg1	intestine					1128:1136	porcine intestine	1120:1136	porcine intestine	1120:1136	(1)H NMR of the intact polysaccharide showed that this unusual batch differed strikingly from other HS preparations obtained from bovine kidney and porcine intestine.
27295282	2	2	theme	rare	357:360	arg1	glucosamine					408:418	N-unsubstituted glucosamine	392:418	N-unsubstituted glucosamine (GlcN)	392:425	HS carbohydrate chains are complex and can contain rare structural components such as N-unsubstituted glucosamine (GlcN).
27295282	2	2	theme	rare	357:360	arg1	components					373:382	rare structural components	357:382	rare structural components such as N-unsubstituted glucosamine (GlcN)	357:425	HS carbohydrate chains are complex and can contain rare structural components such as N-unsubstituted glucosamine (GlcN).
27295282	9	3	theme	Great	1421:1425	arg1	care					1427:1430	Great care	1421:1430	Great care	1421:1430	Great care has to be taken in drawing conclusions from investigations of structural features of HS and specificities of HS interaction with proteins when commercial HS is used without further analysis.
27295282	10	4	theme	community	1752:1760	arg1	members					1726:1732	members	1726:1732	members of the scientific community who have used the present preparation in their studies	1726:1815	Pending the availability of a validated commercial HS reference preparation, our data may be useful to members of the scientific community who have used the present preparation in their studies.
27295282	1	5	theme	many	269:272	arg1	processes					295:303	many important biological processes	269:303	many important biological processes	269:303	Heparan sulfate (HS) polysaccharides are ubiquitous in animal tissues as components of proteoglycans, and they participate in many important biological processes.
27295282	2	6	theme	N-unsubstituted	392:406	arg1	glucosamine					408:418	N-unsubstituted glucosamine	392:418	N-unsubstituted glucosamine (GlcN)	392:425	HS carbohydrate chains are complex and can contain rare structural components such as N-unsubstituted glucosamine (GlcN).
27295282	2	6	theme	N-unsubstituted	392:406	arg1	GlcN					421:424	GlcN	421:424	GlcN	421:424	HS carbohydrate chains are complex and can contain rare structural components such as N-unsubstituted glucosamine (GlcN).
27295282	3	7	from	types	496:500	arg1	invaluable					477:486	invaluable	477:486	invaluable	477:486	Commercially available HS preparations have been invaluable in many types of research activities.
27295282	10	8	used	used	1771:1774	arg2	members					1726:1732	members	1726:1732	members of the scientific community who have used the present preparation in their studies	1726:1815	Pending the availability of a validated commercial HS reference preparation, our data may be useful to members of the scientific community who have used the present preparation in their studies.
27295282	4	9	theme	purchased	682:690	arg1	batch					692:696	a recently purchased batch	671:696	a recently purchased batch of porcine intestinal mucosal HS	671:729	In the course of preparing microarrays to include probes derived from HS oligosaccharides, we found an unusually high content of GlcN residue in a recently purchased batch of porcine intestinal mucosal HS.
27295282	0	10	theme	Heparan	126:132	arg1	Sulfate					134:140	Commercially Available Porcine Intestinal Heparan Sulfate	84:140	Commercially Available Porcine Intestinal Heparan Sulfate	84:140	Abnormally High Content of Free Glucosamine Residues Identified in a Preparation of Commercially Available Porcine Intestinal Heparan Sulfate.
27295282	2	11	theme	HS	306:307	arg1	chains					322:327	HS carbohydrate chains	306:327	HS carbohydrate chains	306:327	HS carbohydrate chains are complex and can contain rare structural components such as N-unsubstituted glucosamine (GlcN).
27295282	5	12	theme	polysaccharide	861:874	arg1	digestion					844:852	heparin lyase III digestion	826:852	heparin lyase III digestion of the polysaccharide	826:874	Composition and sequence analysis by mass spectrometry of the oligosaccharides obtained after heparin lyase III digestion of the polysaccharide indicated two and three GlcN in the tetrasaccharide and hexasaccharide fractions, respectively.
27295282	8	13	used	used	1343:1346	arg2	HS					1330:1331	HS	1330:1331	HS	1330:1331	HS is widely used by the scientific community to investigate HS structures and activities.
27295282	4	14	theme	residue	660:666	arg1	content					644:650	an unusually high content	626:650	an unusually high content of GlcN residue	626:666	In the course of preparing microarrays to include probes derived from HS oligosaccharides, we found an unusually high content of GlcN residue in a recently purchased batch of porcine intestinal mucosal HS.
27295282	4	15	theme	HS	728:729	arg1	batch					692:696	a recently purchased batch	671:696	a recently purchased batch of porcine intestinal mucosal HS	671:729	In the course of preparing microarrays to include probes derived from HS oligosaccharides, we found an unusually high content of GlcN residue in a recently purchased batch of porcine intestinal mucosal HS.
27295282	9	16	with	interaction	1544:1554	arg1	proteins					1561:1568	proteins	1561:1568	proteins when commercial HS is used without further analysis	1561:1620	Great care has to be taken in drawing conclusions from investigations of structural features of HS and specificities of HS interaction with proteins when commercial HS is used without further analysis.
27295282	9	17	theme	structural	1494:1503	arg1	features					1505:1512	structural features	1494:1512	structural features of HS	1494:1518	Great care has to be taken in drawing conclusions from investigations of structural features of HS and specificities of HS interaction with proteins when commercial HS is used without further analysis.
27295282	6	18	dep	NMR	977:979	arg1	1					973:973	1	973:973	1	973:973	(1)H NMR of the intact polysaccharide showed that this unusual batch differed strikingly from other HS preparations obtained from bovine kidney and porcine intestine.
27295282	7	19	theme	GlcN	1164:1167	arg1	content					1153:1159	The very high content	1139:1159	The very high content of GlcN (30%) and low content of GlcNAc (4.2%) determined by disaccharide composition analysis	1139:1254	The very high content of GlcN (30%) and low content of GlcNAc (4.2%) determined by disaccharide composition analysis indicated that N-deacetylation and/or N-desulfation may have taken place.
27295282	1	20	from	ubiquitous	184:193	arg1	tissues					205:211	animal tissues	198:211	animal tissues as components of proteoglycans	198:242	Heparan sulfate (HS) polysaccharides are ubiquitous in animal tissues as components of proteoglycans, and they participate in many important biological processes.
27295282	1	21	theme	Heparan	143:149	arg1	HS					160:161	HS	160:161	HS	160:161	Heparan sulfate (HS) polysaccharides are ubiquitous in animal tissues as components of proteoglycans, and they participate in many important biological processes.
27295282	1	21	theme	Heparan	143:149	arg1	sulfate					151:157	Heparan sulfate	143:157	Heparan sulfate (HS) polysaccharides	143:178	Heparan sulfate (HS) polysaccharides are ubiquitous in animal tissues as components of proteoglycans, and they participate in many important biological processes.
27295282	5	22	theme	tetrasaccharide	912:926	arg1	fractions					947:955	the tetrasaccharide and hexasaccharide fractions	908:955	the tetrasaccharide and hexasaccharide fractions	908:955	Composition and sequence analysis by mass spectrometry of the oligosaccharides obtained after heparin lyase III digestion of the polysaccharide indicated two and three GlcN in the tetrasaccharide and hexasaccharide fractions, respectively.
27295282	6	23	theme	unusual	1027:1033	arg1	batch					1035:1039	this unusual batch	1022:1039	this unusual batch	1022:1039	(1)H NMR of the intact polysaccharide showed that this unusual batch differed strikingly from other HS preparations obtained from bovine kidney and porcine intestine.
27295282	4	24	theme	intestinal	709:718	arg1	HS					728:729	porcine intestinal mucosal HS	701:729	porcine intestinal mucosal HS	701:729	In the course of preparing microarrays to include probes derived from HS oligosaccharides, we found an unusually high content of GlcN residue in a recently purchased batch of porcine intestinal mucosal HS.
27295282	5	25	theme	hexasaccharide	932:945	arg1	fractions					947:955	the tetrasaccharide and hexasaccharide fractions	908:955	the tetrasaccharide and hexasaccharide fractions	908:955	Composition and sequence analysis by mass spectrometry of the oligosaccharides obtained after heparin lyase III digestion of the polysaccharide indicated two and three GlcN in the tetrasaccharide and hexasaccharide fractions, respectively.
27295282	5	26	theme	sequence	748:755	arg1	analysis					757:764	sequence analysis	748:764	sequence analysis	748:764	Composition and sequence analysis by mass spectrometry of the oligosaccharides obtained after heparin lyase III digestion of the polysaccharide indicated two and three GlcN in the tetrasaccharide and hexasaccharide fractions, respectively.
27295282	9	27	theme	interaction	1544:1554	arg1	conclusions					1459:1469	conclusions	1459:1469	conclusions from investigations of structural features of HS	1459:1518	Great care has to be taken in drawing conclusions from investigations of structural features of HS and specificities of HS interaction with proteins when commercial HS is used without further analysis.
27295282	9	27	theme	interaction	1544:1554	arg1	specificities					1524:1536	specificities	1524:1536	specificities of HS interaction with proteins when commercial HS is used without further analysis	1524:1620	Great care has to be taken in drawing conclusions from investigations of structural features of HS and specificities of HS interaction with proteins when commercial HS is used without further analysis.
27295282	6	28	theme	polysaccharide	995:1008	arg1	NMR					977:979	H NMR	975:979	(1)H NMR of the intact polysaccharide	972:1008	(1)H NMR of the intact polysaccharide showed that this unusual batch differed strikingly from other HS preparations obtained from bovine kidney and porcine intestine.
27295282	3	29	theme	many	491:494	arg1	types					496:500	many types	491:500	many types of research activities	491:523	Commercially available HS preparations have been invaluable in many types of research activities.
27295282	10	30	theme	commercial	1663:1672	arg1	preparation					1687:1697	a validated commercial HS reference preparation	1651:1697	a validated commercial HS reference preparation	1651:1697	Pending the availability of a validated commercial HS reference preparation, our data may be useful to members of the scientific community who have used the present preparation in their studies.
27295282	0	31	theme	Porcine	107:113	arg1	Sulfate					134:140	Commercially Available Porcine Intestinal Heparan Sulfate	84:140	Commercially Available Porcine Intestinal Heparan Sulfate	84:140	Abnormally High Content of Free Glucosamine Residues Identified in a Preparation of Commercially Available Porcine Intestinal Heparan Sulfate.
27295282	0	32	theme	Glucosamine	32:42	arg1	Residues					44:51	Free Glucosamine Residues	27:51	Free Glucosamine Residues	27:51	Abnormally High Content of Free Glucosamine Residues Identified in a Preparation of Commercially Available Porcine Intestinal Heparan Sulfate.
27295282	6	33	theme	bovine	1102:1107	arg1	kidney					1109:1114	bovine kidney	1102:1114	bovine kidney	1102:1114	(1)H NMR of the intact polysaccharide showed that this unusual batch differed strikingly from other HS preparations obtained from bovine kidney and porcine intestine.
27295282	3	34	theme	available	441:449	arg1	preparations					454:465	Commercially available HS preparations	428:465	Commercially available HS preparations	428:465	Commercially available HS preparations have been invaluable in many types of research activities.
27295282	7	35	theme	low	1179:1181	arg1	content					1183:1189	low content	1179:1189	low content of GlcNAc (4.2%) determined by disaccharide composition analysis	1179:1254	The very high content of GlcN (30%) and low content of GlcNAc (4.2%) determined by disaccharide composition analysis indicated that N-deacetylation and/or N-desulfation may have taken place.
27295282	1	36	theme	important	274:282	arg1	processes					295:303	many important biological processes	269:303	many important biological processes	269:303	Heparan sulfate (HS) polysaccharides are ubiquitous in animal tissues as components of proteoglycans, and they participate in many important biological processes.
27295282	9	37	theme	commercial	1575:1584	arg1	HS					1586:1587	commercial HS	1575:1587	commercial HS	1575:1587	Great care has to be taken in drawing conclusions from investigations of structural features of HS and specificities of HS interaction with proteins when commercial HS is used without further analysis.
27295282	10	38	theme	scientific	1741:1750	arg1	community					1752:1760	the scientific community	1737:1760	the scientific community	1737:1760	Pending the availability of a validated commercial HS reference preparation, our data may be useful to members of the scientific community who have used the present preparation in their studies.
27295282	6	39	theme	HS	1072:1073	arg1	preparations					1075:1086	other HS preparations	1066:1086	other HS preparations obtained from bovine kidney and porcine intestine	1066:1136	(1)H NMR of the intact polysaccharide showed that this unusual batch differed strikingly from other HS preparations obtained from bovine kidney and porcine intestine.
27295282	10	40	theme	reference	1677:1685	arg1	preparation					1687:1697	a validated commercial HS reference preparation	1651:1697	a validated commercial HS reference preparation	1651:1697	Pending the availability of a validated commercial HS reference preparation, our data may be useful to members of the scientific community who have used the present preparation in their studies.
27295282	0	41	theme	Free	27:30	arg1	Residues					44:51	Free Glucosamine Residues	27:51	Free Glucosamine Residues	27:51	Abnormally High Content of Free Glucosamine Residues Identified in a Preparation of Commercially Available Porcine Intestinal Heparan Sulfate.
27295282	3	42	from	invaluable	477:486	arg1	types					496:500	many types	491:500	many types of research activities	491:523	Commercially available HS preparations have been invaluable in many types of research activities.
27295282	3	43	theme	research	505:512	arg1	activities					514:523	research activities	505:523	research activities	505:523	Commercially available HS preparations have been invaluable in many types of research activities.
27295282	5	44	theme	heparin	826:832	arg1	lyase					834:838	heparin lyase III	826:842	heparin lyase III digestion of the polysaccharide	826:874	Composition and sequence analysis by mass spectrometry of the oligosaccharides obtained after heparin lyase III digestion of the polysaccharide indicated two and three GlcN in the tetrasaccharide and hexasaccharide fractions, respectively.
27295282	7	45	theme	content	1183:1189	arg1	content					1153:1159	The very high content	1139:1159	The very high content of GlcN (30%) and low content of GlcNAc (4.2%) determined by disaccharide composition analysis	1139:1254	The very high content of GlcN (30%) and low content of GlcNAc (4.2%) determined by disaccharide composition analysis indicated that N-deacetylation and/or N-desulfation may have taken place.
27295282	7	46	theme	high	1148:1151	arg1	content					1153:1159	The very high content	1139:1159	The very high content of GlcN (30%) and low content of GlcNAc (4.2%) determined by disaccharide composition analysis	1139:1254	The very high content of GlcN (30%) and low content of GlcNAc (4.2%) determined by disaccharide composition analysis indicated that N-deacetylation and/or N-desulfation may have taken place.
27295282	2	47	theme	structural	362:371	arg1	glucosamine					408:418	N-unsubstituted glucosamine	392:418	N-unsubstituted glucosamine (GlcN)	392:425	HS carbohydrate chains are complex and can contain rare structural components such as N-unsubstituted glucosamine (GlcN).
27295282	2	47	theme	structural	362:371	arg1	components					373:382	rare structural components	357:382	rare structural components such as N-unsubstituted glucosamine (GlcN)	357:425	HS carbohydrate chains are complex and can contain rare structural components such as N-unsubstituted glucosamine (GlcN).
27295282	7	48	theme	disaccharide	1222:1233	arg1	analysis					1247:1254	disaccharide composition analysis	1222:1254	disaccharide composition analysis	1222:1254	The very high content of GlcN (30%) and low content of GlcNAc (4.2%) determined by disaccharide composition analysis indicated that N-deacetylation and/or N-desulfation may have taken place.
27295282	4	49	theme	HS	596:597	arg1	oligosaccharides					599:614	HS oligosaccharides	596:614	HS oligosaccharides	596:614	In the course of preparing microarrays to include probes derived from HS oligosaccharides, we found an unusually high content of GlcN residue in a recently purchased batch of porcine intestinal mucosal HS.
27295282	4	50	attach	derived	583:589	arg2	probes					576:581	probes	576:581	probes derived from HS oligosaccharides	576:614	In the course of preparing microarrays to include probes derived from HS oligosaccharides, we found an unusually high content of GlcN residue in a recently purchased batch of porcine intestinal mucosal HS.
27295282	4	50	attach	derived	583:589	arg1	oligosaccharides					599:614	HS oligosaccharides	596:614	HS oligosaccharides	596:614	In the course of preparing microarrays to include probes derived from HS oligosaccharides, we found an unusually high content of GlcN residue in a recently purchased batch of porcine intestinal mucosal HS.
27295282	0	51	theme	Residues	44:51	arg1	Content					16:22	Abnormally High Content	0:22	Abnormally High Content of Free Glucosamine Residues	0:51	Abnormally High Content of Free Glucosamine Residues Identified in a Preparation of Commercially Available Porcine Intestinal Heparan Sulfate.
27295282	10	52	theme	present	1780:1786	arg1	preparation					1788:1798	the present preparation	1776:1798	the present preparation in their studies	1776:1815	Pending the availability of a validated commercial HS reference preparation, our data may be useful to members of the scientific community who have used the present preparation in their studies.
27295282	6	53	theme	intact	988:993	arg1	polysaccharide					995:1008	the intact polysaccharide	984:1008	the intact polysaccharide	984:1008	(1)H NMR of the intact polysaccharide showed that this unusual batch differed strikingly from other HS preparations obtained from bovine kidney and porcine intestine.
27295282	0	54	theme	Sulfate	134:140	arg1	Preparation					69:79	a Preparation	67:79	a Preparation of Commercially Available Porcine Intestinal Heparan Sulfate	67:140	Abnormally High Content of Free Glucosamine Residues Identified in a Preparation of Commercially Available Porcine Intestinal Heparan Sulfate.
27295282	2	55	theme	carbohydrate	309:320	arg1	chains					322:327	HS carbohydrate chains	306:327	HS carbohydrate chains	306:327	HS carbohydrate chains are complex and can contain rare structural components such as N-unsubstituted glucosamine (GlcN).
27295282	1	56	theme	animal	198:203	arg1	tissues					205:211	animal tissues	198:211	animal tissues as components of proteoglycans	198:242	Heparan sulfate (HS) polysaccharides are ubiquitous in animal tissues as components of proteoglycans, and they participate in many important biological processes.
27295282	1	57	theme	sulfate	151:157	arg1	polysaccharides					164:178	Heparan sulfate (HS) polysaccharides	143:178	Heparan sulfate (HS) polysaccharides	143:178	Heparan sulfate (HS) polysaccharides are ubiquitous in animal tissues as components of proteoglycans, and they participate in many important biological processes.
27295282	6	58	theme	H	975:975	arg1	NMR					977:979	H NMR	975:979	(1)H NMR of the intact polysaccharide	972:1008	(1)H NMR of the intact polysaccharide showed that this unusual batch differed strikingly from other HS preparations obtained from bovine kidney and porcine intestine.
27295282	4	59	theme	GlcN	655:658	arg1	residue					660:666	GlcN residue	655:666	GlcN residue	655:666	In the course of preparing microarrays to include probes derived from HS oligosaccharides, we found an unusually high content of GlcN residue in a recently purchased batch of porcine intestinal mucosal HS.
27295282	7	60	theme	GlcNAc	1194:1199	arg1	content					1183:1189	low content	1179:1189	low content of GlcNAc (4.2%) determined by disaccharide composition analysis	1179:1254	The very high content of GlcN (30%) and low content of GlcNAc (4.2%) determined by disaccharide composition analysis indicated that N-deacetylation and/or N-desulfation may have taken place.
27295282	7	60	theme	GlcNAc	1194:1199	arg1	GlcN					1164:1167	GlcN	1164:1167	GlcN (30%)	1164:1173	The very high content of GlcN (30%) and low content of GlcNAc (4.2%) determined by disaccharide composition analysis indicated that N-deacetylation and/or N-desulfation may have taken place.
27295282	7	60	theme	GlcNAc	1194:1199	arg1	%					1172:1172	30%	1170:1172	30%	1170:1172	The very high content of GlcN (30%) and low content of GlcNAc (4.2%) determined by disaccharide composition analysis indicated that N-deacetylation and/or N-desulfation may have taken place.
27295282	4	61	theme	mucosal	720:726	arg1	HS					728:729	porcine intestinal mucosal HS	701:729	porcine intestinal mucosal HS	701:729	In the course of preparing microarrays to include probes derived from HS oligosaccharides, we found an unusually high content of GlcN residue in a recently purchased batch of porcine intestinal mucosal HS.
27295282	1	62	from	tissues	205:211	arg1	ubiquitous					184:193	ubiquitous	184:193	ubiquitous	184:193	Heparan sulfate (HS) polysaccharides are ubiquitous in animal tissues as components of proteoglycans, and they participate in many important biological processes.
27295282	9	63	theme	features	1505:1512	arg1	investigations					1476:1489	investigations	1476:1489	investigations of structural features of HS	1476:1518	Great care has to be taken in drawing conclusions from investigations of structural features of HS and specificities of HS interaction with proteins when commercial HS is used without further analysis.
27295282	5	64	theme	mass	769:772	arg1	spectrometry					774:785	mass spectrometry	769:785	mass spectrometry of the oligosaccharides obtained after heparin lyase III digestion of the polysaccharide	769:874	Composition and sequence analysis by mass spectrometry of the oligosaccharides obtained after heparin lyase III digestion of the polysaccharide indicated two and three GlcN in the tetrasaccharide and hexasaccharide fractions, respectively.
27295282	9	65	theme	HS	1517:1518	arg1	features					1505:1512	structural features	1494:1512	structural features of HS	1494:1518	Great care has to be taken in drawing conclusions from investigations of structural features of HS and specificities of HS interaction with proteins when commercial HS is used without further analysis.
27295282	4	66	theme	porcine	701:707	arg1	HS					728:729	porcine intestinal mucosal HS	701:729	porcine intestinal mucosal HS	701:729	In the course of preparing microarrays to include probes derived from HS oligosaccharides, we found an unusually high content of GlcN residue in a recently purchased batch of porcine intestinal mucosal HS.
27295282	0	67	theme	Available	97:105	arg1	Sulfate					134:140	Commercially Available Porcine Intestinal Heparan Sulfate	84:140	Commercially Available Porcine Intestinal Heparan Sulfate	84:140	Abnormally High Content of Free Glucosamine Residues Identified in a Preparation of Commercially Available Porcine Intestinal Heparan Sulfate.
27295282	2	68	contain	contain	349:355	arg2	components					373:382	rare structural components	357:382	rare structural components such as N-unsubstituted glucosamine (GlcN)	357:425	HS carbohydrate chains are complex and can contain rare structural components such as N-unsubstituted glucosamine (GlcN).
27295282	2	68	contain	contain	349:355	arg1	chains					322:327	HS carbohydrate chains	306:327	HS carbohydrate chains	306:327	HS carbohydrate chains are complex and can contain rare structural components such as N-unsubstituted glucosamine (GlcN).
27295282	2	68	contain	contain	349:355	arg2	glucosamine					408:418	N-unsubstituted glucosamine	392:418	N-unsubstituted glucosamine (GlcN)	392:425	HS carbohydrate chains are complex and can contain rare structural components such as N-unsubstituted glucosamine (GlcN).
27295282	9	69	theme	HS	1541:1542	arg1	interaction					1544:1554	HS interaction	1541:1554	HS interaction with proteins when commercial HS is used without further analysis	1541:1620	Great care has to be taken in drawing conclusions from investigations of structural features of HS and specificities of HS interaction with proteins when commercial HS is used without further analysis.
27295282	0	70	theme	Intestinal	115:124	arg1	Sulfate					134:140	Commercially Available Porcine Intestinal Heparan Sulfate	84:140	Commercially Available Porcine Intestinal Heparan Sulfate	84:140	Abnormally High Content of Free Glucosamine Residues Identified in a Preparation of Commercially Available Porcine Intestinal Heparan Sulfate.
27295282	10	71	from	preparation	1788:1798	arg1	studies					1809:1815	their studies	1803:1815	their studies	1803:1815	Pending the availability of a validated commercial HS reference preparation, our data may be useful to members of the scientific community who have used the present preparation in their studies.
27295282	10	72	theme	validated	1653:1661	arg1	preparation					1687:1697	a validated commercial HS reference preparation	1651:1697	a validated commercial HS reference preparation	1651:1697	Pending the availability of a validated commercial HS reference preparation, our data may be useful to members of the scientific community who have used the present preparation in their studies.
27295282	5	73	theme	lyase	834:838	arg1	digestion					844:852	heparin lyase III digestion	826:852	heparin lyase III digestion of the polysaccharide	826:874	Composition and sequence analysis by mass spectrometry of the oligosaccharides obtained after heparin lyase III digestion of the polysaccharide indicated two and three GlcN in the tetrasaccharide and hexasaccharide fractions, respectively.
27295282	8	74	theme	scientific	1355:1364	arg1	community					1366:1374	the scientific community	1351:1374	the scientific community	1351:1374	HS is widely used by the scientific community to investigate HS structures and activities.
27295282	3	75	theme	HS	451:452	arg1	preparations					454:465	Commercially available HS preparations	428:465	Commercially available HS preparations	428:465	Commercially available HS preparations have been invaluable in many types of research activities.
27295282	1	76	theme	biological	284:293	arg1	processes					295:303	many important biological processes	269:303	many important biological processes	269:303	Heparan sulfate (HS) polysaccharides are ubiquitous in animal tissues as components of proteoglycans, and they participate in many important biological processes.
27295282	10	77	theme	preparation	1687:1697	arg1	availability					1635:1646	the availability	1631:1646	the availability of a validated commercial HS reference preparation	1631:1697	Pending the availability of a validated commercial HS reference preparation, our data may be useful to members of the scientific community who have used the present preparation in their studies.
27295282	5	78	theme	oligosaccharides	794:809	arg1	spectrometry					774:785	mass spectrometry	769:785	mass spectrometry of the oligosaccharides obtained after heparin lyase III digestion of the polysaccharide	769:874	Composition and sequence analysis by mass spectrometry of the oligosaccharides obtained after heparin lyase III digestion of the polysaccharide indicated two and three GlcN in the tetrasaccharide and hexasaccharide fractions, respectively.
27295282	6	79	theme	other	1066:1070	arg1	preparations					1075:1086	other HS preparations	1066:1086	other HS preparations obtained from bovine kidney and porcine intestine	1066:1136	(1)H NMR of the intact polysaccharide showed that this unusual batch differed strikingly from other HS preparations obtained from bovine kidney and porcine intestine.
27295282	8	80	theme	HS	1391:1392	arg1	structures					1394:1403	HS structures	1391:1403	HS structures	1391:1403	HS is widely used by the scientific community to investigate HS structures and activities.
27295282	10	81	theme	HS	1674:1675	arg1	preparation					1687:1697	a validated commercial HS reference preparation	1651:1697	a validated commercial HS reference preparation	1651:1697	Pending the availability of a validated commercial HS reference preparation, our data may be useful to members of the scientific community who have used the present preparation in their studies.
27295282	9	82	theme	further	1605:1611	arg1	analysis					1613:1620	further analysis	1605:1620	further analysis	1605:1620	Great care has to be taken in drawing conclusions from investigations of structural features of HS and specificities of HS interaction with proteins when commercial HS is used without further analysis.
27295282	9	83	from	investigations	1476:1489	arg1	conclusions					1459:1469	conclusions	1459:1469	conclusions from investigations of structural features of HS	1459:1518	Great care has to be taken in drawing conclusions from investigations of structural features of HS and specificities of HS interaction with proteins when commercial HS is used without further analysis.
27295282	9	83	from	investigations	1476:1489	arg1	specificities					1524:1536	specificities	1524:1536	specificities of HS interaction with proteins when commercial HS is used without further analysis	1524:1620	Great care has to be taken in drawing conclusions from investigations of structural features of HS and specificities of HS interaction with proteins when commercial HS is used without further analysis.
27295282	3	84	theme	activities	514:523	arg1	types					496:500	many types	491:500	many types of research activities	491:523	Commercially available HS preparations have been invaluable in many types of research activities.
27295282	1	85	theme	proteoglycans	230:242	arg1	components					216:225	components	216:225	components of proteoglycans	216:242	Heparan sulfate (HS) polysaccharides are ubiquitous in animal tissues as components of proteoglycans, and they participate in many important biological processes.
27295282	7	86	theme	composition	1235:1245	arg1	analysis					1247:1254	disaccharide composition analysis	1222:1254	disaccharide composition analysis	1222:1254	The very high content of GlcN (30%) and low content of GlcNAc (4.2%) determined by disaccharide composition analysis indicated that N-deacetylation and/or N-desulfation may have taken place.
27295282	4	87	theme	high	639:642	arg1	content					644:650	an unusually high content	626:650	an unusually high content of GlcN residue	626:666	In the course of preparing microarrays to include probes derived from HS oligosaccharides, we found an unusually high content of GlcN residue in a recently purchased batch of porcine intestinal mucosal HS.
27295282	0	88	theme	High	11:14	arg1	Content					16:22	Abnormally High Content	0:22	Abnormally High Content of Free Glucosamine Residues	0:51	Abnormally High Content of Free Glucosamine Residues Identified in a Preparation of Commercially Available Porcine Intestinal Heparan Sulfate.
28549863	9	0	from	fibers	1433:1438	arg1	MCC					1416:1418	the isolated MCC	1403:1418	the isolated MCC from roselle fibers	1403:1438	Thus, the isolated MCC from roselle fibers will be going to use as reinforcing element in green composites and may be a precursor for future roselle derived nanocellulose, and thus a promising subject in nanocomposite research.
28549863	9	0	from	fibers	1433:1438	arg1	going					1448:1452	going	1448:1452	going	1448:1452	Thus, the isolated MCC from roselle fibers will be going to use as reinforcing element in green composites and may be a precursor for future roselle derived nanocellulose, and thus a promising subject in nanocomposite research.
28549863	1	1	theme	roselle	149:155	arg1	fiber					157:161	roselle fiber	149:161	roselle fiber	149:161	In this study, microcrystalline cellulose (MCC) was extracted from roselle fiber through acid hydrolysis treatment and its properties were compared with those of commercially available MCC.
28549863	8	2	contain	had	1270:1272	arg2	stability					1289:1297	better thermal stability	1274:1297	better thermal stability	1274:1297	TGA and DSC results indicated that the roselle MCC had better thermal stability than the roselle pulp, whereas it had poorer thermal stability in comparison with commercial MCC.
28549863	8	2	contain	had	1270:1272	arg1	MCC					1266:1268	the roselle MCC	1254:1268	the roselle MCC	1254:1268	TGA and DSC results indicated that the roselle MCC had better thermal stability than the roselle pulp, whereas it had poorer thermal stability in comparison with commercial MCC.
28549863	4	3	theme	scanning	701:708	arg1	SEM					731:733	SEM	731:733	SEM	731:733	Rough surface and slight aggregation of MCC were observed by scanning electron microscopy (SEM).
28549863	4	3	theme	scanning	701:708	arg1	microscopy					719:728	scanning electron microscopy	701:728	scanning electron microscopy (SEM)	701:734	Rough surface and slight aggregation of MCC were observed by scanning electron microscopy (SEM).
28549863	8	4	contain	had	1333:1335	arg2	stability					1352:1360	poorer thermal stability	1337:1360	poorer thermal stability	1337:1360	TGA and DSC results indicated that the roselle MCC had better thermal stability than the roselle pulp, whereas it had poorer thermal stability in comparison with commercial MCC.
28549863	8	4	contain	had	1333:1335	arg1	it					1330:1331	it	1330:1331	it	1330:1331	TGA and DSC results indicated that the roselle MCC had better thermal stability than the roselle pulp, whereas it had poorer thermal stability in comparison with commercial MCC.
28549863	1	5	theme	available	257:265	arg1	MCC					267:269	commercially available MCC	244:269	commercially available MCC	244:269	In this study, microcrystalline cellulose (MCC) was extracted from roselle fiber through acid hydrolysis treatment and its properties were compared with those of commercially available MCC.
28549863	9	6	from	element	1476:1482	arg1	composites					1493:1502	green composites	1487:1502	green composites	1487:1502	Thus, the isolated MCC from roselle fibers will be going to use as reinforcing element in green composites and may be a precursor for future roselle derived nanocellulose, and thus a promising subject in nanocomposite research.
28549863	9	7	theme	isolated	1407:1414	arg1	MCC					1416:1418	the isolated MCC	1403:1418	the isolated MCC from roselle fibers	1403:1438	Thus, the isolated MCC from roselle fibers will be going to use as reinforcing element in green composites and may be a precursor for future roselle derived nanocellulose, and thus a promising subject in nanocomposite research.
28549863	9	7	theme	isolated	1407:1414	arg1	going					1448:1452	going	1448:1452	going	1448:1452	Thus, the isolated MCC from roselle fibers will be going to use as reinforcing element in green composites and may be a precursor for future roselle derived nanocellulose, and thus a promising subject in nanocomposite research.
28549863	7	8	dep	increased	1076:1084	arg1	having					1145:1150	having	1145:1150	having a slightly higher crystallinity than that of commercial MCC (74%)	1145:1216	X-ray diffraction (XRD) showed the crystallinity increased from 63% in roselle pulp to 78% in roselle MCC, the latter having a slightly higher crystallinity than that of commercial MCC (74%).
28549863	2	9	theme	morphological	296:308	arg1	characteristics					310:324	The physicochemical and morphological characteristics	272:324	The physicochemical and morphological characteristics	272:324	The physicochemical and morphological characteristics, elemental composition, size distribution, crystallinity and thermal properties of the obtained MCC were analyzed in this work.
28549863	8	10	theme	better	1274:1279	arg1	stability					1289:1297	better thermal stability	1274:1297	better thermal stability	1274:1297	TGA and DSC results indicated that the roselle MCC had better thermal stability than the roselle pulp, whereas it had poorer thermal stability in comparison with commercial MCC.
28549863	5	11	theme	Energy	737:742	arg1	analysis					767:774	Energy dispersive X-ray (EDX) analysis	737:774	Energy dispersive X-ray (EDX) analysis	737:774	Energy dispersive X-ray (EDX) analysis showed that pure MCC with small quantities of residues and impurities was obtained, with a similar elemental composition to that of commercial MCC.
28549863	5	12	with	MCC	793:795	arg1	quantities					808:817	small quantities	802:817	small quantities of residues and impurities	802:844	Energy dispersive X-ray (EDX) analysis showed that pure MCC with small quantities of residues and impurities was obtained, with a similar elemental composition to that of commercial MCC.
28549863	4	13	theme	electron	710:717	arg1	SEM					731:733	SEM	731:733	SEM	731:733	Rough surface and slight aggregation of MCC were observed by scanning electron microscopy (SEM).
28549863	4	13	theme	electron	710:717	arg1	microscopy					719:728	scanning electron microscopy	701:728	scanning electron microscopy (SEM)	701:734	Rough surface and slight aggregation of MCC were observed by scanning electron microscopy (SEM).
28549863	7	14	theme	commercial	1197:1206	arg1	MCC					1208:1210	commercial MCC	1197:1210	commercial MCC (74%)	1197:1216	X-ray diffraction (XRD) showed the crystallinity increased from 63% in roselle pulp to 78% in roselle MCC, the latter having a slightly higher crystallinity than that of commercial MCC (74%).
28549863	7	14	theme	commercial	1197:1206	arg1	%					1215:1215	74%	1213:1215	74%	1213:1215	X-ray diffraction (XRD) showed the crystallinity increased from 63% in roselle pulp to 78% in roselle MCC, the latter having a slightly higher crystallinity than that of commercial MCC (74%).
28549863	5	15	theme	dispersive	744:753	arg1	analysis					767:774	Energy dispersive X-ray (EDX) analysis	737:774	Energy dispersive X-ray (EDX) analysis	737:774	Energy dispersive X-ray (EDX) analysis showed that pure MCC with small quantities of residues and impurities was obtained, with a similar elemental composition to that of commercial MCC.
28549863	2	16	theme	physicochemical	276:290	arg1	characteristics					310:324	The physicochemical and morphological characteristics	272:324	The physicochemical and morphological characteristics	272:324	The physicochemical and morphological characteristics, elemental composition, size distribution, crystallinity and thermal properties of the obtained MCC were analyzed in this work.
28549863	2	17	theme	MCC	422:424	arg1	distribution					355:366	size distribution	350:366	size distribution	350:366	The physicochemical and morphological characteristics, elemental composition, size distribution, crystallinity and thermal properties of the obtained MCC were analyzed in this work.
28549863	2	17	theme	MCC	422:424	arg1	properties					395:404	thermal properties	387:404	thermal properties	387:404	The physicochemical and morphological characteristics, elemental composition, size distribution, crystallinity and thermal properties of the obtained MCC were analyzed in this work.
28549863	2	17	theme	MCC	422:424	arg1	crystallinity					369:381	crystallinity	369:381	crystallinity	369:381	The physicochemical and morphological characteristics, elemental composition, size distribution, crystallinity and thermal properties of the obtained MCC were analyzed in this work.
28549863	2	17	theme	MCC	422:424	arg1	characteristics					310:324	The physicochemical and morphological characteristics	272:324	The physicochemical and morphological characteristics	272:324	The physicochemical and morphological characteristics, elemental composition, size distribution, crystallinity and thermal properties of the obtained MCC were analyzed in this work.
28549863	2	17	theme	MCC	422:424	arg1	composition					337:347	elemental composition	327:347	elemental composition	327:347	The physicochemical and morphological characteristics, elemental composition, size distribution, crystallinity and thermal properties of the obtained MCC were analyzed in this work.
28549863	5	18	theme	X-ray	755:759	arg1	analysis					767:774	Energy dispersive X-ray (EDX) analysis	737:774	Energy dispersive X-ray (EDX) analysis	737:774	Energy dispersive X-ray (EDX) analysis showed that pure MCC with small quantities of residues and impurities was obtained, with a similar elemental composition to that of commercial MCC.
28549863	7	19	from	%	1116:1116	arg1	MCC					1129:1131	roselle MCC	1121:1131	roselle MCC	1121:1131	X-ray diffraction (XRD) showed the crystallinity increased from 63% in roselle pulp to 78% in roselle MCC, the latter having a slightly higher crystallinity than that of commercial MCC (74%).
28549863	7	20	theme	roselle	1098:1104	arg1	pulp					1106:1109	roselle pulp	1098:1109	roselle pulp	1098:1109	X-ray diffraction (XRD) showed the crystallinity increased from 63% in roselle pulp to 78% in roselle MCC, the latter having a slightly higher crystallinity than that of commercial MCC (74%).
28549863	6	21	theme	particle	997:1004	arg1	PSA					1021:1023	PSA	1021:1023	PSA	1021:1023	A mean diameter of approximately 44.28μm was measured for MCC by using a particle size analyzer (PSA).
28549863	6	21	theme	particle	997:1004	arg1	analyzer					1011:1018	a particle size analyzer	995:1018	a particle size analyzer (PSA)	995:1024	A mean diameter of approximately 44.28μm was measured for MCC by using a particle size analyzer (PSA).
28549863	7	22	dep	showed	1051:1056	arg1	increased					1076:1084	increased	1076:1084	increased	1076:1084	X-ray diffraction (XRD) showed the crystallinity increased from 63% in roselle pulp to 78% in roselle MCC, the latter having a slightly higher crystallinity than that of commercial MCC (74%).
28549863	2	23	theme	obtained	413:420	arg1	MCC					422:424	the obtained MCC	409:424	the obtained MCC	409:424	The physicochemical and morphological characteristics, elemental composition, size distribution, crystallinity and thermal properties of the obtained MCC were analyzed in this work.
28549863	1	24	theme	hydrolysis	176:185	arg1	treatment					187:195	acid hydrolysis treatment	171:195	acid hydrolysis treatment	171:195	In this study, microcrystalline cellulose (MCC) was extracted from roselle fiber through acid hydrolysis treatment and its properties were compared with those of commercially available MCC.
28549863	5	25	theme	small	802:806	arg1	quantities					808:817	small quantities	802:817	small quantities of residues and impurities	802:844	Energy dispersive X-ray (EDX) analysis showed that pure MCC with small quantities of residues and impurities was obtained, with a similar elemental composition to that of commercial MCC.
28549863	8	26	with	comparison	1365:1374	arg1	MCC					1392:1394	commercial MCC	1381:1394	commercial MCC	1381:1394	TGA and DSC results indicated that the roselle MCC had better thermal stability than the roselle pulp, whereas it had poorer thermal stability in comparison with commercial MCC.
28549863	2	27	theme	elemental	327:335	arg1	composition					337:347	elemental composition	327:347	elemental composition	327:347	The physicochemical and morphological characteristics, elemental composition, size distribution, crystallinity and thermal properties of the obtained MCC were analyzed in this work.
28549863	4	28	theme	MCC	680:682	arg1	surface					646:652	Rough surface	640:652	Rough surface	640:652	Rough surface and slight aggregation of MCC were observed by scanning electron microscopy (SEM).
28549863	4	28	theme	MCC	680:682	arg1	aggregation					665:675	slight aggregation	658:675	slight aggregation	658:675	Rough surface and slight aggregation of MCC were observed by scanning electron microscopy (SEM).
28549863	6	29	theme	44.28μm	957:963	arg1	diameter					931:938	A mean diameter	924:938	A mean diameter of approximately 44.28μm	924:963	A mean diameter of approximately 44.28μm was measured for MCC by using a particle size analyzer (PSA).
28549863	8	30	theme	thermal	1344:1350	arg1	stability					1352:1360	poorer thermal stability	1337:1360	poorer thermal stability	1337:1360	TGA and DSC results indicated that the roselle MCC had better thermal stability than the roselle pulp, whereas it had poorer thermal stability in comparison with commercial MCC.
28549863	9	31	theme	green	1487:1491	arg1	composites					1493:1502	green composites	1487:1502	green composites	1487:1502	Thus, the isolated MCC from roselle fibers will be going to use as reinforcing element in green composites and may be a precursor for future roselle derived nanocellulose, and thus a promising subject in nanocomposite research.
28549863	1	32	theme	microcrystalline	97:112	arg1	MCC					125:127	MCC	125:127	MCC	125:127	In this study, microcrystalline cellulose (MCC) was extracted from roselle fiber through acid hydrolysis treatment and its properties were compared with those of commercially available MCC.
28549863	1	32	theme	microcrystalline	97:112	arg1	cellulose					114:122	microcrystalline cellulose	97:122	microcrystalline cellulose (MCC)	97:128	In this study, microcrystalline cellulose (MCC) was extracted from roselle fiber through acid hydrolysis treatment and its properties were compared with those of commercially available MCC.
28549863	9	33	theme	reinforcing	1464:1474	arg1	element					1476:1482	reinforcing element	1464:1482	reinforcing element in green composites	1464:1502	Thus, the isolated MCC from roselle fibers will be going to use as reinforcing element in green composites and may be a precursor for future roselle derived nanocellulose, and thus a promising subject in nanocomposite research.
28549863	5	34	theme	residues	822:829	arg1	quantities					808:817	small quantities	802:817	small quantities of residues and impurities	802:844	Energy dispersive X-ray (EDX) analysis showed that pure MCC with small quantities of residues and impurities was obtained, with a similar elemental composition to that of commercial MCC.
28549863	9	35	from	roselle	1538:1544	arg1	research					1615:1622	nanocomposite research	1601:1622	nanocomposite research	1601:1622	Thus, the isolated MCC from roselle fibers will be going to use as reinforcing element in green composites and may be a precursor for future roselle derived nanocellulose, and thus a promising subject in nanocomposite research.
28549863	8	36	theme	roselle	1258:1264	arg1	MCC					1266:1268	the roselle MCC	1254:1268	the roselle MCC	1254:1268	TGA and DSC results indicated that the roselle MCC had better thermal stability than the roselle pulp, whereas it had poorer thermal stability in comparison with commercial MCC.
28549863	8	37	theme	poorer	1337:1342	arg1	stability					1352:1360	poorer thermal stability	1337:1360	poorer thermal stability	1337:1360	TGA and DSC results indicated that the roselle MCC had better thermal stability than the roselle pulp, whereas it had poorer thermal stability in comparison with commercial MCC.
28549863	8	38	theme	DSC	1227:1229	arg1	results					1231:1237	TGA and DSC results	1219:1237	TGA and DSC results	1219:1237	TGA and DSC results indicated that the roselle MCC had better thermal stability than the roselle pulp, whereas it had poorer thermal stability in comparison with commercial MCC.
28549863	4	39	theme	Rough	640:644	arg1	surface					646:652	Rough surface	640:652	Rough surface	640:652	Rough surface and slight aggregation of MCC were observed by scanning electron microscopy (SEM).
28549863	0	40	theme	cellulose	51:59	arg1	characterization					14:29	characterization	14:29	characterization	14:29	Isolation and characterization of microcrystalline cellulose from roselle fibers.
28549863	0	40	theme	cellulose	51:59	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation and characterization of microcrystalline cellulose from roselle fibers.
28549863	5	41	theme	impurities	835:844	arg1	quantities					808:817	small quantities	802:817	small quantities of residues and impurities	802:844	Energy dispersive X-ray (EDX) analysis showed that pure MCC with small quantities of residues and impurities was obtained, with a similar elemental composition to that of commercial MCC.
28549863	3	42	theme	MCC	607:609	arg1	spectrum					591:598	the spectrum	587:598	the spectrum of the MCC prepared from roselle fiber	587:637	Fourier transform infrared spectroscopy (FTIR) analysis provided clear evidence that the characteristic peak of lignin was absent in the spectrum of the MCC prepared from roselle fiber.
28549863	3	43	from	spectrum	591:598	arg1	peak					558:561	the characteristic peak	539:561	the characteristic peak of lignin	539:571	Fourier transform infrared spectroscopy (FTIR) analysis provided clear evidence that the characteristic peak of lignin was absent in the spectrum of the MCC prepared from roselle fiber.
28549863	3	43	from	spectrum	591:598	arg1	absent					577:582	absent	577:582	absent	577:582	Fourier transform infrared spectroscopy (FTIR) analysis provided clear evidence that the characteristic peak of lignin was absent in the spectrum of the MCC prepared from roselle fiber.
28549863	4	44	theme	slight	658:663	arg1	aggregation					665:675	slight aggregation	658:675	slight aggregation	658:675	Rough surface and slight aggregation of MCC were observed by scanning electron microscopy (SEM).
28549863	0	45	theme	microcrystalline	34:49	arg1	cellulose					51:59	microcrystalline cellulose	34:59	microcrystalline cellulose	34:59	Isolation and characterization of microcrystalline cellulose from roselle fibers.
28549863	7	46	theme	X-ray	1027:1031	arg1	XRD					1046:1048	XRD	1046:1048	XRD	1046:1048	X-ray diffraction (XRD) showed the crystallinity increased from 63% in roselle pulp to 78% in roselle MCC, the latter having a slightly higher crystallinity than that of commercial MCC (74%).
28549863	7	46	theme	X-ray	1027:1031	arg1	diffraction					1033:1043	X-ray diffraction	1027:1043	X-ray diffraction (XRD)	1027:1049	X-ray diffraction (XRD) showed the crystallinity increased from 63% in roselle pulp to 78% in roselle MCC, the latter having a slightly higher crystallinity than that of commercial MCC (74%).
28549863	3	47	theme	spectroscopy	481:492	arg1	analysis					501:508	spectroscopy (FTIR) analysis	481:508	spectroscopy (FTIR) analysis	481:508	Fourier transform infrared spectroscopy (FTIR) analysis provided clear evidence that the characteristic peak of lignin was absent in the spectrum of the MCC prepared from roselle fiber.
28549863	0	48	theme	roselle	66:72	arg1	fibers					74:79	roselle fibers	66:79	roselle fibers	66:79	Isolation and characterization of microcrystalline cellulose from roselle fibers.
28549863	6	49	theme	mean	926:929	arg1	diameter					931:938	A mean diameter	924:938	A mean diameter of approximately 44.28μm	924:963	A mean diameter of approximately 44.28μm was measured for MCC by using a particle size analyzer (PSA).
28549863	0	50	from	fibers	74:79	arg1	characterization					14:29	characterization	14:29	characterization	14:29	Isolation and characterization of microcrystalline cellulose from roselle fibers.
28549863	0	50	from	fibers	74:79	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation and characterization of microcrystalline cellulose from roselle fibers.
28549863	3	51	dep	Fourier	454:460	arg1	transform					462:470	transform	462:470	transform infrared spectroscopy (FTIR) analysis	462:508	Fourier transform infrared spectroscopy (FTIR) analysis provided clear evidence that the characteristic peak of lignin was absent in the spectrum of the MCC prepared from roselle fiber.
28549863	8	52	theme	TGA	1219:1221	arg1	results					1231:1237	TGA and DSC results	1219:1237	TGA and DSC results	1219:1237	TGA and DSC results indicated that the roselle MCC had better thermal stability than the roselle pulp, whereas it had poorer thermal stability in comparison with commercial MCC.
28549863	9	53	theme	promising	1580:1588	arg1	subject					1590:1596	a promising subject	1578:1596	a promising subject in nanocomposite research	1578:1622	Thus, the isolated MCC from roselle fibers will be going to use as reinforcing element in green composites and may be a precursor for future roselle derived nanocellulose, and thus a promising subject in nanocomposite research.
28549863	8	54	theme	roselle	1308:1314	arg1	pulp					1316:1319	the roselle pulp	1304:1319	the roselle pulp	1304:1319	TGA and DSC results indicated that the roselle MCC had better thermal stability than the roselle pulp, whereas it had poorer thermal stability in comparison with commercial MCC.
28549863	7	55	from	%	1093:1093	arg1	pulp					1106:1109	roselle pulp	1098:1109	roselle pulp	1098:1109	X-ray diffraction (XRD) showed the crystallinity increased from 63% in roselle pulp to 78% in roselle MCC, the latter having a slightly higher crystallinity than that of commercial MCC (74%).
28549863	9	56	theme	roselle	1425:1431	arg1	fibers					1433:1438	roselle fibers	1425:1438	roselle fibers	1425:1438	Thus, the isolated MCC from roselle fibers will be going to use as reinforcing element in green composites and may be a precursor for future roselle derived nanocellulose, and thus a promising subject in nanocomposite research.
28549863	3	57	dep	transform	462:470	arg1	infrared					472:479	infrared	472:479	transform infrared spectroscopy (FTIR) analysis	462:508	Fourier transform infrared spectroscopy (FTIR) analysis provided clear evidence that the characteristic peak of lignin was absent in the spectrum of the MCC prepared from roselle fiber.
28549863	9	58	theme	future	1531:1536	arg1	roselle					1538:1544	future roselle	1531:1544	future roselle derived nanocellulose	1531:1566	Thus, the isolated MCC from roselle fibers will be going to use as reinforcing element in green composites and may be a precursor for future roselle derived nanocellulose, and thus a promising subject in nanocomposite research.
28549863	3	59	theme	clear	519:523	arg1	evidence					525:532	clear evidence that the characteristic peak of lignin was absent in the spectrum of the MCC prepared from roselle fiber	519:637	clear evidence that the characteristic peak of lignin was absent in the spectrum of the MCC prepared from roselle fiber	519:637	Fourier transform infrared spectroscopy (FTIR) analysis provided clear evidence that the characteristic peak of lignin was absent in the spectrum of the MCC prepared from roselle fiber.
28549863	3	60	from	absent	577:582	arg1	spectrum					591:598	the spectrum	587:598	the spectrum of the MCC prepared from roselle fiber	587:637	Fourier transform infrared spectroscopy (FTIR) analysis provided clear evidence that the characteristic peak of lignin was absent in the spectrum of the MCC prepared from roselle fiber.
28549863	9	61	theme	nanocomposite	1601:1613	arg1	research					1615:1622	nanocomposite research	1601:1622	nanocomposite research	1601:1622	Thus, the isolated MCC from roselle fibers will be going to use as reinforcing element in green composites and may be a precursor for future roselle derived nanocellulose, and thus a promising subject in nanocomposite research.
28549863	5	62	theme	pure	788:791	arg1	MCC					793:795	pure MCC	788:795	pure MCC with small quantities of residues and impurities	788:844	Energy dispersive X-ray (EDX) analysis showed that pure MCC with small quantities of residues and impurities was obtained, with a similar elemental composition to that of commercial MCC.
28549863	2	63	theme	size	350:353	arg1	distribution					355:366	size distribution	350:366	size distribution	350:366	The physicochemical and morphological characteristics, elemental composition, size distribution, crystallinity and thermal properties of the obtained MCC were analyzed in this work.
28549863	8	64	theme	thermal	1281:1287	arg1	stability					1289:1297	better thermal stability	1274:1297	better thermal stability	1274:1297	TGA and DSC results indicated that the roselle MCC had better thermal stability than the roselle pulp, whereas it had poorer thermal stability in comparison with commercial MCC.
28549863	6	65	theme	size	1006:1009	arg1	PSA					1021:1023	PSA	1021:1023	PSA	1021:1023	A mean diameter of approximately 44.28μm was measured for MCC by using a particle size analyzer (PSA).
28549863	6	65	theme	size	1006:1009	arg1	analyzer					1011:1018	a particle size analyzer	995:1018	a particle size analyzer (PSA)	995:1024	A mean diameter of approximately 44.28μm was measured for MCC by using a particle size analyzer (PSA).
28549863	8	66	theme	commercial	1381:1390	arg1	MCC					1392:1394	commercial MCC	1381:1394	commercial MCC	1381:1394	TGA and DSC results indicated that the roselle MCC had better thermal stability than the roselle pulp, whereas it had poorer thermal stability in comparison with commercial MCC.
28549863	3	67	theme	lignin	566:571	arg1	peak					558:561	the characteristic peak	539:561	the characteristic peak of lignin	539:571	Fourier transform infrared spectroscopy (FTIR) analysis provided clear evidence that the characteristic peak of lignin was absent in the spectrum of the MCC prepared from roselle fiber.
28549863	3	67	theme	lignin	566:571	arg1	absent					577:582	absent	577:582	absent	577:582	Fourier transform infrared spectroscopy (FTIR) analysis provided clear evidence that the characteristic peak of lignin was absent in the spectrum of the MCC prepared from roselle fiber.
28549863	2	68	theme	thermal	387:393	arg1	properties					395:404	thermal properties	387:404	thermal properties	387:404	The physicochemical and morphological characteristics, elemental composition, size distribution, crystallinity and thermal properties of the obtained MCC were analyzed in this work.
28549863	5	69	theme	commercial	908:917	arg1	MCC					919:921	commercial MCC	908:921	commercial MCC	908:921	Energy dispersive X-ray (EDX) analysis showed that pure MCC with small quantities of residues and impurities was obtained, with a similar elemental composition to that of commercial MCC.
28549863	5	70	theme	elemental	875:883	arg1	composition					885:895	a similar elemental composition	865:895	a similar elemental composition to that of commercial MCC	865:921	Energy dispersive X-ray (EDX) analysis showed that pure MCC with small quantities of residues and impurities was obtained, with a similar elemental composition to that of commercial MCC.
28549863	5	71	theme	similar	867:873	arg1	composition					885:895	a similar elemental composition	865:895	a similar elemental composition to that of commercial MCC	865:921	Energy dispersive X-ray (EDX) analysis showed that pure MCC with small quantities of residues and impurities was obtained, with a similar elemental composition to that of commercial MCC.
28549863	1	72	theme	acid	171:174	arg1	treatment					187:195	acid hydrolysis treatment	171:195	acid hydrolysis treatment	171:195	In this study, microcrystalline cellulose (MCC) was extracted from roselle fiber through acid hydrolysis treatment and its properties were compared with those of commercially available MCC.
28549863	9	73	from	subject	1590:1596	arg1	research					1615:1622	nanocomposite research	1601:1622	nanocomposite research	1601:1622	Thus, the isolated MCC from roselle fibers will be going to use as reinforcing element in green composites and may be a precursor for future roselle derived nanocellulose, and thus a promising subject in nanocomposite research.
28549863	5	74	theme	EDX	762:764	arg1	analysis					767:774	Energy dispersive X-ray (EDX) analysis	737:774	Energy dispersive X-ray (EDX) analysis	737:774	Energy dispersive X-ray (EDX) analysis showed that pure MCC with small quantities of residues and impurities was obtained, with a similar elemental composition to that of commercial MCC.
28549863	7	75	theme	roselle	1121:1127	arg1	MCC					1129:1131	roselle MCC	1121:1131	roselle MCC	1121:1131	X-ray diffraction (XRD) showed the crystallinity increased from 63% in roselle pulp to 78% in roselle MCC, the latter having a slightly higher crystallinity than that of commercial MCC (74%).
28549863	3	76	theme	roselle	625:631	arg1	fiber					633:637	roselle fiber	625:637	roselle fiber	625:637	Fourier transform infrared spectroscopy (FTIR) analysis provided clear evidence that the characteristic peak of lignin was absent in the spectrum of the MCC prepared from roselle fiber.
28549863	3	77	theme	characteristic	543:556	arg1	peak					558:561	the characteristic peak	539:561	the characteristic peak of lignin	539:571	Fourier transform infrared spectroscopy (FTIR) analysis provided clear evidence that the characteristic peak of lignin was absent in the spectrum of the MCC prepared from roselle fiber.
28549863	3	77	theme	characteristic	543:556	arg1	absent					577:582	absent	577:582	absent	577:582	Fourier transform infrared spectroscopy (FTIR) analysis provided clear evidence that the characteristic peak of lignin was absent in the spectrum of the MCC prepared from roselle fiber.
28549863	7	78	theme	higher	1163:1168	arg1	crystallinity					1170:1182	a slightly higher crystallinity	1152:1182	a slightly higher crystallinity than that of commercial MCC (74%)	1152:1216	X-ray diffraction (XRD) showed the crystallinity increased from 63% in roselle pulp to 78% in roselle MCC, the latter having a slightly higher crystallinity than that of commercial MCC (74%).
24885467	8	0	theme	fengycin	1157:1164	arg1	lipopeptides					1106:1117	lipopeptides	1106:1117	lipopeptides	1106:1117	In dextrose, sucrose and glycerol supplemented MSM, strain AR2 produced lipopeptides as a mixture of surfactin, iturin and fengycin.
24885467	8	0	theme	fengycin	1157:1164	arg1	mixture					1124:1130	a mixture	1122:1130	a mixture of surfactin, iturin and fengycin	1122:1164	In dextrose, sucrose and glycerol supplemented MSM, strain AR2 produced lipopeptides as a mixture of surfactin, iturin and fengycin.
24885467	13	1	theme	lipopeptide	1741:1751	arg1	biosurfactant					1753:1765	lipopeptide biosurfactant	1741:1765	lipopeptide biosurfactant	1741:1765	Sucrose is the most suitable carbon source for production of lipopeptide biosurfactant with antifungal activity.
24885467	4	2	theme	source	527:532	arg1	effect					486:491	the qualitative and quantitative effect	453:491	the qualitative and quantitative effect of different water soluble carbon source on the biosurfactant produced by Bacillus amylofaciens strain AR2	453:598	The present study investigates the qualitative and quantitative effect of different water soluble carbon source on the biosurfactant produced by Bacillus amylofaciens strain AR2.
24885467	10	3	theme	compositional	1276:1288	arg1	variation					1290:1298	This substrate dependent compositional variation	1251:1298	This substrate dependent compositional variation in the lipopeptides	1251:1318	This substrate dependent compositional variation in the lipopeptides significantly influenced antifungal activity.
24885467	0	4	theme	AR2	71:73	arg1	activity					40:47	vitro antifungal activity	23:47	vitro antifungal activity of Bacillus sp strain AR2	23:73	Substrate dependent in vitro antifungal activity of Bacillus sp strain AR2.
24885467	0	5	from	dependent	10:18	arg1	activity					40:47	vitro antifungal activity	23:47	vitro antifungal activity of Bacillus sp strain AR2	23:73	Substrate dependent in vitro antifungal activity of Bacillus sp strain AR2.
24885467	13	6	theme	biosurfactant	1753:1765	arg1	production					1727:1736	production	1727:1736	production of lipopeptide biosurfactant with antifungal activity	1727:1790	Sucrose is the most suitable carbon source for production of lipopeptide biosurfactant with antifungal activity.
24885467	4	7	theme	present	426:432	arg1	study					434:438	The present study	422:438	The present study	422:438	The present study investigates the qualitative and quantitative effect of different water soluble carbon source on the biosurfactant produced by Bacillus amylofaciens strain AR2.
24885467	4	8	theme	different	496:504	arg1	source					527:532	different water soluble carbon source	496:532	different water soluble carbon source	496:532	The present study investigates the qualitative and quantitative effect of different water soluble carbon source on the biosurfactant produced by Bacillus amylofaciens strain AR2.
24885467	10	9	theme	dependent	1266:1274	arg1	variation					1290:1298	This substrate dependent compositional variation	1251:1298	This substrate dependent compositional variation in the lipopeptides	1251:1318	This substrate dependent compositional variation in the lipopeptides significantly influenced antifungal activity.
24885467	4	10	dep	different	496:504	arg1	soluble					512:518	soluble	512:518	soluble	512:518	The present study investigates the qualitative and quantitative effect of different water soluble carbon source on the biosurfactant produced by Bacillus amylofaciens strain AR2.
24885467	3	11	theme	biosurfactant	370:382	arg1	diversity					357:365	Structural and compositional diversity	328:365	Structural and compositional diversity of biosurfactant	328:382	Structural and compositional diversity of biosurfactant is unambiguously substrate dependent.
24885467	6	12	dep	supplemented	768:779	arg1	sucrose					760:766	sucrose	760:766	sucrose	760:766	Maximum biosurfactant production was observed in the sucrose supplemented minimal salt medium (MSM).
24885467	6	13	located	observed	744:751	arg2	production					729:738	Maximum biosurfactant production	707:738	Maximum biosurfactant production	707:738	Maximum biosurfactant production was observed in the sucrose supplemented minimal salt medium (MSM).
24885467	6	13	located	observed	744:751	arg1	MSM					802:804	MSM	802:804	MSM	802:804	Maximum biosurfactant production was observed in the sucrose supplemented minimal salt medium (MSM).
24885467	6	13	located	observed	744:751	arg1	medium					794:799	the sucrose supplemented minimal salt medium	756:799	the sucrose supplemented minimal salt medium (MSM)	756:805	Maximum biosurfactant production was observed in the sucrose supplemented minimal salt medium (MSM).
24885467	9	14	theme	maltose	1195:1201	arg1	presence					1183:1190	the presence	1179:1190	the presence of maltose, lactose and sorbitol	1179:1223	However, in the presence of maltose, lactose and sorbitol only iturin was produced.
24885467	10	15	theme	substrate	1256:1264	arg1	variation					1290:1298	This substrate dependent compositional variation	1251:1298	This substrate dependent compositional variation in the lipopeptides	1251:1318	This substrate dependent compositional variation in the lipopeptides significantly influenced antifungal activity.
24885467	4	16	theme	carbon	520:525	arg1	source					527:532	different water soluble carbon source	496:532	different water soluble carbon source	496:532	The present study investigates the qualitative and quantitative effect of different water soluble carbon source on the biosurfactant produced by Bacillus amylofaciens strain AR2.
24885467	13	17	theme	antifungal	1772:1781	arg1	activity					1783:1790	antifungal activity	1772:1790	antifungal activity	1772:1790	Sucrose is the most suitable carbon source for production of lipopeptide biosurfactant with antifungal activity.
24885467	11	18	theme	dextrose	1431:1438	arg1	MSM					1446:1448	dextrose based MSM	1431:1448	dextrose based MSM	1431:1448	Lipopeptides produced by strain AR2 while growing on sucrose and dextrose based MSM was observed to be most efficient as an antifungal agent.
24885467	7	19	theme	range	951:955	arg1	80-110 mg/l					957:967	the range 80-110 mg/l	947:967	the range 80-110 mg/l	947:967	Strain AR2 exhibited carbon source dependent surface tension reduction in the range of 30-37 mN/m, critical micelle concentration (CMC) in the range 80-110 mg/l and emulsification index (EI24 kerosene) in the range of 32-66%.
24885467	7	20	theme	micelle	916:922	arg1	CMC					939:941	CMC	939:941	CMC	939:941	Strain AR2 exhibited carbon source dependent surface tension reduction in the range of 30-37 mN/m, critical micelle concentration (CMC) in the range 80-110 mg/l and emulsification index (EI24 kerosene) in the range of 32-66%.
24885467	7	20	theme	micelle	916:922	arg1	concentration					924:936	critical micelle concentration	907:936	critical micelle concentration (CMC) in the range 80-110 mg/l	907:967	Strain AR2 exhibited carbon source dependent surface tension reduction in the range of 30-37 mN/m, critical micelle concentration (CMC) in the range 80-110 mg/l and emulsification index (EI24 kerosene) in the range of 32-66%.
24885467	13	21	with	production	1727:1736	arg1	activity					1783:1790	antifungal activity	1772:1790	antifungal activity	1772:1790	Sucrose is the most suitable carbon source for production of lipopeptide biosurfactant with antifungal activity.
24885467	5	22	theme	soluble	683:689	arg1	sources					698:704	water soluble carbon sources	677:704	water soluble carbon sources	677:704	RESULTS Strain AR2 produced lipopeptide type biosurfactant while growing on water soluble carbon sources.
24885467	9	23	theme	lactose	1204:1210	arg1	presence					1183:1190	the presence	1179:1190	the presence of maltose, lactose and sorbitol	1179:1223	However, in the presence of maltose, lactose and sorbitol only iturin was produced.
24885467	6	24	theme	salt	789:792	arg1	MSM					802:804	MSM	802:804	MSM	802:804	Maximum biosurfactant production was observed in the sucrose supplemented minimal salt medium (MSM).
24885467	6	24	theme	salt	789:792	arg1	medium					794:799	the sucrose supplemented minimal salt medium	756:799	the sucrose supplemented minimal salt medium (MSM)	756:805	Maximum biosurfactant production was observed in the sucrose supplemented minimal salt medium (MSM).
24885467	0	25	from	activity	40:47	arg1	dependent					10:18	dependent	10:18	dependent	10:18	Substrate dependent in vitro antifungal activity of Bacillus sp strain AR2.
24885467	5	26	theme	carbon	691:696	arg1	sources					698:704	water soluble carbon sources	677:704	water soluble carbon sources	677:704	RESULTS Strain AR2 produced lipopeptide type biosurfactant while growing on water soluble carbon sources.
24885467	7	27	theme	critical	907:914	arg1	CMC					939:941	CMC	939:941	CMC	939:941	Strain AR2 exhibited carbon source dependent surface tension reduction in the range of 30-37 mN/m, critical micelle concentration (CMC) in the range 80-110 mg/l and emulsification index (EI24 kerosene) in the range of 32-66%.
24885467	7	27	theme	critical	907:914	arg1	concentration					924:936	critical micelle concentration	907:936	critical micelle concentration (CMC) in the range 80-110 mg/l	907:967	Strain AR2 exhibited carbon source dependent surface tension reduction in the range of 30-37 mN/m, critical micelle concentration (CMC) in the range 80-110 mg/l and emulsification index (EI24 kerosene) in the range of 32-66%.
24885467	12	28	theme	biosurfactant	1589:1601	arg1	production					1603:1612	biosurfactant production	1589:1612	biosurfactant production	1589:1612	CONCLUSIONS These results suggest that carbon source provided for the growth and biosurfactant production not only influences the yield but also the type of biosurfactant.
24885467	6	29	theme	minimal	781:787	arg1	MSM					802:804	MSM	802:804	MSM	802:804	Maximum biosurfactant production was observed in the sucrose supplemented minimal salt medium (MSM).
24885467	6	29	theme	minimal	781:787	arg1	medium					794:799	the sucrose supplemented minimal salt medium	756:799	the sucrose supplemented minimal salt medium (MSM)	756:805	Maximum biosurfactant production was observed in the sucrose supplemented minimal salt medium (MSM).
24885467	1	30	theme	potential	173:181	arg1	potential					173:181	potential	173:181	potential	173:181	BACKGROUND Biosurfactants are a structurally diverse group of secondary metabolites with lots of potential to serve mankind.
24885467	1	30	theme	potential	173:181	arg1	lots					165:168	lots	165:168	lots of potential	165:181	BACKGROUND Biosurfactants are a structurally diverse group of secondary metabolites with lots of potential to serve mankind.
24885467	3	31	theme	Structural	328:337	arg1	diversity					357:365	Structural and compositional diversity	328:365	Structural and compositional diversity of biosurfactant	328:382	Structural and compositional diversity of biosurfactant is unambiguously substrate dependent.
24885467	5	32	theme	lipopeptide	629:639	arg1	biosurfactant					646:658	lipopeptide type biosurfactant	629:658	lipopeptide type biosurfactant	629:658	RESULTS Strain AR2 produced lipopeptide type biosurfactant while growing on water soluble carbon sources.
24885467	1	33	theme	BACKGROUND	76:85	arg1	group					129:133	a structurally diverse group	106:133	a structurally diverse group of secondary metabolites with lots of potential to serve mankind	106:198	BACKGROUND Biosurfactants are a structurally diverse group of secondary metabolites with lots of potential to serve mankind.
24885467	1	33	theme	BACKGROUND	76:85	arg1	Biosurfactants					87:100	BACKGROUND Biosurfactants	76:100	BACKGROUND Biosurfactants	76:100	BACKGROUND Biosurfactants are a structurally diverse group of secondary metabolites with lots of potential to serve mankind.
24885467	1	33	theme	BACKGROUND	76:85	arg1	metabolites					148:158	secondary metabolites	138:158	secondary metabolites	138:158	BACKGROUND Biosurfactants are a structurally diverse group of secondary metabolites with lots of potential to serve mankind.
24885467	6	34	theme	supplemented	768:779	arg1	MSM					802:804	MSM	802:804	MSM	802:804	Maximum biosurfactant production was observed in the sucrose supplemented minimal salt medium (MSM).
24885467	6	34	theme	supplemented	768:779	arg1	medium					794:799	the sucrose supplemented minimal salt medium	756:799	the sucrose supplemented minimal salt medium (MSM)	756:805	Maximum biosurfactant production was observed in the sucrose supplemented minimal salt medium (MSM).
24885467	11	35	theme	strain	1391:1396	arg1	AR2					1398:1400	strain AR2	1391:1400	strain AR2	1391:1400	Lipopeptides produced by strain AR2 while growing on sucrose and dextrose based MSM was observed to be most efficient as an antifungal agent.
24885467	11	36	theme	based	1440:1444	arg1	MSM					1446:1448	dextrose based MSM	1431:1448	dextrose based MSM	1431:1448	Lipopeptides produced by strain AR2 while growing on sucrose and dextrose based MSM was observed to be most efficient as an antifungal agent.
24885467	5	37	theme	type	641:644	arg1	biosurfactant					646:658	lipopeptide type biosurfactant	629:658	lipopeptide type biosurfactant	629:658	RESULTS Strain AR2 produced lipopeptide type biosurfactant while growing on water soluble carbon sources.
24885467	2	38	theme	particular	304:313	arg1	application					315:325	a particular application	302:325	a particular application	302:325	Depending upon the structure and composition they may exhibit properties that make them suitable for a particular application.
24885467	7	39	theme	mN/m	901:904	arg1	range					886:890	the range	882:890	the range of 30-37 mN/m	882:904	Strain AR2 exhibited carbon source dependent surface tension reduction in the range of 30-37 mN/m, critical micelle concentration (CMC) in the range 80-110 mg/l and emulsification index (EI24 kerosene) in the range of 32-66%.
24885467	0	40	theme	vitro	23:27	arg1	activity					40:47	vitro antifungal activity	23:47	vitro antifungal activity of Bacillus sp strain AR2	23:73	Substrate dependent in vitro antifungal activity of Bacillus sp strain AR2.
24885467	7	41	from	reduction	869:877	arg1	80-110 mg/l					957:967	the range 80-110 mg/l	947:967	the range 80-110 mg/l	947:967	Strain AR2 exhibited carbon source dependent surface tension reduction in the range of 30-37 mN/m, critical micelle concentration (CMC) in the range 80-110 mg/l and emulsification index (EI24 kerosene) in the range of 32-66%.
24885467	7	41	from	reduction	869:877	arg1	range					886:890	the range	882:890	the range of 30-37 mN/m	882:904	Strain AR2 exhibited carbon source dependent surface tension reduction in the range of 30-37 mN/m, critical micelle concentration (CMC) in the range 80-110 mg/l and emulsification index (EI24 kerosene) in the range of 32-66%.
24885467	10	42	from	variation	1290:1298	arg1	lipopeptides					1307:1318	the lipopeptides	1303:1318	the lipopeptides	1303:1318	This substrate dependent compositional variation in the lipopeptides significantly influenced antifungal activity.
24885467	12	43	theme	biosurfactant	1665:1677	arg1	type					1657:1660	the type	1653:1660	the type of biosurfactant	1653:1677	CONCLUSIONS These results suggest that carbon source provided for the growth and biosurfactant production not only influences the yield but also the type of biosurfactant.
24885467	2	44	dep	structure	220:228	arg1	the					216:218	the	216:218	the	216:218	Depending upon the structure and composition they may exhibit properties that make them suitable for a particular application.
24885467	2	44	dep	structure	220:228	arg1	exhibit					255:261	exhibit	255:261	may exhibit properties that make them suitable for a particular application	251:325	Depending upon the structure and composition they may exhibit properties that make them suitable for a particular application.
24885467	12	45	theme	carbon	1547:1552	arg1	source					1554:1559	carbon source	1547:1559	carbon source provided for the growth and biosurfactant production	1547:1612	CONCLUSIONS These results suggest that carbon source provided for the growth and biosurfactant production not only influences the yield but also the type of biosurfactant.
24885467	9	46	theme	sorbitol	1216:1223	arg1	presence					1183:1190	the presence	1179:1190	the presence of maltose, lactose and sorbitol	1179:1223	However, in the presence of maltose, lactose and sorbitol only iturin was produced.
24885467	10	47	theme	antifungal	1345:1354	arg1	activity					1356:1363	antifungal activity	1345:1363	antifungal activity	1345:1363	This substrate dependent compositional variation in the lipopeptides significantly influenced antifungal activity.
24885467	8	48	theme	strain	1086:1091	arg1	AR2					1093:1095	strain AR2	1086:1095	strain AR2	1086:1095	In dextrose, sucrose and glycerol supplemented MSM, strain AR2 produced lipopeptides as a mixture of surfactin, iturin and fengycin.
24885467	13	49	theme	suitable	1700:1707	arg1	Sucrose					1680:1686	Sucrose	1680:1686	Sucrose	1680:1686	Sucrose is the most suitable carbon source for production of lipopeptide biosurfactant with antifungal activity.
24885467	13	49	theme	suitable	1700:1707	arg1	source					1716:1721	the most suitable carbon source	1691:1721	the most suitable carbon source for production of lipopeptide biosurfactant with antifungal activity	1691:1790	Sucrose is the most suitable carbon source for production of lipopeptide biosurfactant with antifungal activity.
24885467	11	50	theme	antifungal	1490:1499	arg1	agent					1501:1505	an antifungal agent	1487:1505	an antifungal agent	1487:1505	Lipopeptides produced by strain AR2 while growing on sucrose and dextrose based MSM was observed to be most efficient as an antifungal agent.
24885467	0	51	theme	antifungal	29:38	arg1	activity					40:47	vitro antifungal activity	23:47	vitro antifungal activity of Bacillus sp strain AR2	23:73	Substrate dependent in vitro antifungal activity of Bacillus sp strain AR2.
24885467	4	52	theme	amylofaciens	576:587	arg1	AR2					596:598	Bacillus amylofaciens strain AR2	567:598	Bacillus amylofaciens strain AR2	567:598	The present study investigates the qualitative and quantitative effect of different water soluble carbon source on the biosurfactant produced by Bacillus amylofaciens strain AR2.
24885467	4	53	from	effect	486:491	arg1	biosurfactant					541:553	the biosurfactant	537:553	the biosurfactant produced by Bacillus amylofaciens strain AR2	537:598	The present study investigates the qualitative and quantitative effect of different water soluble carbon source on the biosurfactant produced by Bacillus amylofaciens strain AR2.
24885467	7	54	theme	dependent	843:851	arg1	reduction					869:877	carbon source dependent surface tension reduction	829:877	carbon source dependent surface tension reduction in the range of 30-37 mN/m	829:904	Strain AR2 exhibited carbon source dependent surface tension reduction in the range of 30-37 mN/m, critical micelle concentration (CMC) in the range 80-110 mg/l and emulsification index (EI24 kerosene) in the range of 32-66%.
24885467	0	55	theme	Bacillus	52:59	arg1	AR2					71:73	Bacillus sp strain AR2	52:73	Bacillus sp strain AR2	52:73	Substrate dependent in vitro antifungal activity of Bacillus sp strain AR2.
24885467	4	56	theme	Bacillus	567:574	arg1	AR2					596:598	Bacillus amylofaciens strain AR2	567:598	Bacillus amylofaciens strain AR2	567:598	The present study investigates the qualitative and quantitative effect of different water soluble carbon source on the biosurfactant produced by Bacillus amylofaciens strain AR2.
24885467	8	57	theme	surfactin	1135:1143	arg1	lipopeptides					1106:1117	lipopeptides	1106:1117	lipopeptides	1106:1117	In dextrose, sucrose and glycerol supplemented MSM, strain AR2 produced lipopeptides as a mixture of surfactin, iturin and fengycin.
24885467	8	57	theme	surfactin	1135:1143	arg1	mixture					1124:1130	a mixture	1122:1130	a mixture of surfactin, iturin and fengycin	1122:1164	In dextrose, sucrose and glycerol supplemented MSM, strain AR2 produced lipopeptides as a mixture of surfactin, iturin and fengycin.
24885467	0	58	theme	sp	61:62	arg1	AR2					71:73	Bacillus sp strain AR2	52:73	Bacillus sp strain AR2	52:73	Substrate dependent in vitro antifungal activity of Bacillus sp strain AR2.
24885467	5	59	theme	RESULTS	601:607	arg1	AR2					616:618	RESULTS Strain AR2	601:618	RESULTS Strain AR2	601:618	RESULTS Strain AR2 produced lipopeptide type biosurfactant while growing on water soluble carbon sources.
24885467	7	60	from	index	988:992	arg1	80-110 mg/l					957:967	the range 80-110 mg/l	947:967	the range 80-110 mg/l	947:967	Strain AR2 exhibited carbon source dependent surface tension reduction in the range of 30-37 mN/m, critical micelle concentration (CMC) in the range 80-110 mg/l and emulsification index (EI24 kerosene) in the range of 32-66%.
24885467	7	60	from	index	988:992	arg1	range					886:890	the range	882:890	the range of 30-37 mN/m	882:904	Strain AR2 exhibited carbon source dependent surface tension reduction in the range of 30-37 mN/m, critical micelle concentration (CMC) in the range 80-110 mg/l and emulsification index (EI24 kerosene) in the range of 32-66%.
24885467	13	61	theme	carbon	1709:1714	arg1	Sucrose					1680:1686	Sucrose	1680:1686	Sucrose	1680:1686	Sucrose is the most suitable carbon source for production of lipopeptide biosurfactant with antifungal activity.
24885467	13	61	theme	carbon	1709:1714	arg1	source					1716:1721	the most suitable carbon source	1691:1721	the most suitable carbon source for production of lipopeptide biosurfactant with antifungal activity	1691:1790	Sucrose is the most suitable carbon source for production of lipopeptide biosurfactant with antifungal activity.
24885467	7	62	theme	surface	853:859	arg1	reduction					869:877	carbon source dependent surface tension reduction	829:877	carbon source dependent surface tension reduction in the range of 30-37 mN/m	829:904	Strain AR2 exhibited carbon source dependent surface tension reduction in the range of 30-37 mN/m, critical micelle concentration (CMC) in the range 80-110 mg/l and emulsification index (EI24 kerosene) in the range of 32-66%.
24885467	4	63	theme	qualitative	457:467	arg1	effect					486:491	the qualitative and quantitative effect	453:491	the qualitative and quantitative effect of different water soluble carbon source on the biosurfactant produced by Bacillus amylofaciens strain AR2	453:598	The present study investigates the qualitative and quantitative effect of different water soluble carbon source on the biosurfactant produced by Bacillus amylofaciens strain AR2.
24885467	5	64	theme	Strain	609:614	arg1	AR2					616:618	RESULTS Strain AR2	601:618	RESULTS Strain AR2	601:618	RESULTS Strain AR2 produced lipopeptide type biosurfactant while growing on water soluble carbon sources.
24885467	1	65	theme	diverse	121:127	arg1	group					129:133	a structurally diverse group	106:133	a structurally diverse group of secondary metabolites with lots of potential to serve mankind	106:198	BACKGROUND Biosurfactants are a structurally diverse group of secondary metabolites with lots of potential to serve mankind.
24885467	1	65	theme	diverse	121:127	arg1	metabolites					148:158	secondary metabolites	138:158	secondary metabolites	138:158	BACKGROUND Biosurfactants are a structurally diverse group of secondary metabolites with lots of potential to serve mankind.
24885467	1	65	theme	diverse	121:127	arg1	Biosurfactants					87:100	BACKGROUND Biosurfactants	76:100	BACKGROUND Biosurfactants	76:100	BACKGROUND Biosurfactants are a structurally diverse group of secondary metabolites with lots of potential to serve mankind.
24885467	4	66	theme	strain	589:594	arg1	AR2					596:598	Bacillus amylofaciens strain AR2	567:598	Bacillus amylofaciens strain AR2	567:598	The present study investigates the qualitative and quantitative effect of different water soluble carbon source on the biosurfactant produced by Bacillus amylofaciens strain AR2.
24885467	8	67	theme	iturin	1146:1151	arg1	lipopeptides					1106:1117	lipopeptides	1106:1117	lipopeptides	1106:1117	In dextrose, sucrose and glycerol supplemented MSM, strain AR2 produced lipopeptides as a mixture of surfactin, iturin and fengycin.
24885467	8	67	theme	iturin	1146:1151	arg1	mixture					1124:1130	a mixture	1122:1130	a mixture of surfactin, iturin and fengycin	1122:1164	In dextrose, sucrose and glycerol supplemented MSM, strain AR2 produced lipopeptides as a mixture of surfactin, iturin and fengycin.
24885467	7	68	theme	emulsification	973:986	arg1	index					988:992	emulsification index	973:992	emulsification index (EI24 kerosene)	973:1008	Strain AR2 exhibited carbon source dependent surface tension reduction in the range of 30-37 mN/m, critical micelle concentration (CMC) in the range 80-110 mg/l and emulsification index (EI24 kerosene) in the range of 32-66%.
24885467	7	68	theme	emulsification	973:986	arg1	kerosene					1000:1007	EI24 kerosene	995:1007	EI24 kerosene	995:1007	Strain AR2 exhibited carbon source dependent surface tension reduction in the range of 30-37 mN/m, critical micelle concentration (CMC) in the range 80-110 mg/l and emulsification index (EI24 kerosene) in the range of 32-66%.
24885467	4	69	theme	quantitative	473:484	arg1	effect					486:491	the qualitative and quantitative effect	453:491	the qualitative and quantitative effect of different water soluble carbon source on the biosurfactant produced by Bacillus amylofaciens strain AR2	453:598	The present study investigates the qualitative and quantitative effect of different water soluble carbon source on the biosurfactant produced by Bacillus amylofaciens strain AR2.
24885467	7	70	theme	%	1031:1031	arg1	range					1017:1021	the range	1013:1021	the range of 32-66%	1013:1031	Strain AR2 exhibited carbon source dependent surface tension reduction in the range of 30-37 mN/m, critical micelle concentration (CMC) in the range 80-110 mg/l and emulsification index (EI24 kerosene) in the range of 32-66%.
24885467	6	71	theme	biosurfactant	715:727	arg1	production					729:738	Maximum biosurfactant production	707:738	Maximum biosurfactant production	707:738	Maximum biosurfactant production was observed in the sucrose supplemented minimal salt medium (MSM).
24885467	7	72	theme	carbon	829:834	arg1	source					836:841	carbon source	829:841	carbon source	829:841	Strain AR2 exhibited carbon source dependent surface tension reduction in the range of 30-37 mN/m, critical micelle concentration (CMC) in the range 80-110 mg/l and emulsification index (EI24 kerosene) in the range of 32-66%.
24885467	0	73	theme	strain	64:69	arg1	AR2					71:73	Bacillus sp strain AR2	52:73	Bacillus sp strain AR2	52:73	Substrate dependent in vitro antifungal activity of Bacillus sp strain AR2.
24885467	7	74	from	concentration	924:936	arg1	80-110 mg/l					957:967	the range 80-110 mg/l	947:967	the range 80-110 mg/l	947:967	Strain AR2 exhibited carbon source dependent surface tension reduction in the range of 30-37 mN/m, critical micelle concentration (CMC) in the range 80-110 mg/l and emulsification index (EI24 kerosene) in the range of 32-66%.
24885467	7	74	from	concentration	924:936	arg1	range					886:890	the range	882:890	the range of 30-37 mN/m	882:904	Strain AR2 exhibited carbon source dependent surface tension reduction in the range of 30-37 mN/m, critical micelle concentration (CMC) in the range 80-110 mg/l and emulsification index (EI24 kerosene) in the range of 32-66%.
24885467	1	75	theme	secondary	138:146	arg1	metabolites					148:158	secondary metabolites	138:158	secondary metabolites	138:158	BACKGROUND Biosurfactants are a structurally diverse group of secondary metabolites with lots of potential to serve mankind.
24885467	6	76	theme	Maximum	707:713	arg1	production					729:738	Maximum biosurfactant production	707:738	Maximum biosurfactant production	707:738	Maximum biosurfactant production was observed in the sucrose supplemented minimal salt medium (MSM).
24885467	7	77	theme	Strain	808:813	arg1	AR2					815:817	Strain AR2	808:817	Strain AR2	808:817	Strain AR2 exhibited carbon source dependent surface tension reduction in the range of 30-37 mN/m, critical micelle concentration (CMC) in the range 80-110 mg/l and emulsification index (EI24 kerosene) in the range of 32-66%.
24885467	3	78	theme	compositional	343:355	arg1	diversity					357:365	Structural and compositional diversity	328:365	Structural and compositional diversity of biosurfactant	328:382	Structural and compositional diversity of biosurfactant is unambiguously substrate dependent.
24885467	12	79	dep	CONCLUSIONS	1508:1518	arg1	suggest					1534:1540	suggest	1534:1540	suggest that carbon source provided for the growth and biosurfactant production not only influences the yield but also the type of biosurfactant	1534:1677	CONCLUSIONS These results suggest that carbon source provided for the growth and biosurfactant production not only influences the yield but also the type of biosurfactant.
24885467	1	80	theme	metabolites	148:158	arg1	group					129:133	a structurally diverse group	106:133	a structurally diverse group of secondary metabolites with lots of potential to serve mankind	106:198	BACKGROUND Biosurfactants are a structurally diverse group of secondary metabolites with lots of potential to serve mankind.
24885467	1	80	theme	metabolites	148:158	arg1	metabolites					148:158	secondary metabolites	138:158	secondary metabolites	138:158	BACKGROUND Biosurfactants are a structurally diverse group of secondary metabolites with lots of potential to serve mankind.
24885467	1	80	theme	metabolites	148:158	arg1	Biosurfactants					87:100	BACKGROUND Biosurfactants	76:100	BACKGROUND Biosurfactants	76:100	BACKGROUND Biosurfactants are a structurally diverse group of secondary metabolites with lots of potential to serve mankind.
24885467	7	81	theme	tension	861:867	arg1	reduction					869:877	carbon source dependent surface tension reduction	829:877	carbon source dependent surface tension reduction in the range of 30-37 mN/m	829:904	Strain AR2 exhibited carbon source dependent surface tension reduction in the range of 30-37 mN/m, critical micelle concentration (CMC) in the range 80-110 mg/l and emulsification index (EI24 kerosene) in the range of 32-66%.
26876864	6	0	theme	DMA	1256:1258	arg1	results					1260:1266	DMA results	1256:1266	DMA results	1256:1266	Both storage modulus and glass translation temperature of PBS/PLA blend are increased by DCP and PBS-g-CNC, which is proved by DMA results, showing a weak molecular segment mobility of PBS/PLA matrix.
26876864	5	1	theme	high	1024:1027	arg1	impact					1042:1047	high Notched Izod impact strength and moduli	1024:1067	impact	1042:1047	PBS/PLA blends modified by DCP have high Notched Izod impact strength and moduli, and the values are increased by the addition of PBS-g-CNC.
26876864	7	2	dep	crystallization	1364:1378	arg1	temperature					1380:1390	temperature	1380:1390	temperature	1380:1390	The addition of DCP decreases the crystallization temperature and crystallinity of PBS/PLA composite, but increases the thermal stability of composites, mostly because of the crosslink effect of DCP on PBS/PLA matrix.
26876864	1	3	theme	butylene	131:138	arg1	Poly					126:129	Poly	126:129	Poly(butylene succinate) (PBS)/polylactic acid (PLA) blends modified with dicumyl peroxide (DCP)	126:221	Poly(butylene succinate) (PBS)/polylactic acid (PLA) blends modified with dicumyl peroxide (DCP) were reinforced by PBS-g-cellulose nanocrystal (CNC) through melt mixing.
26876864	1	3	theme	butylene	131:138	arg1	succinate					140:148	butylene succinate	131:148	butylene succinate	131:148	Poly(butylene succinate) (PBS)/polylactic acid (PLA) blends modified with dicumyl peroxide (DCP) were reinforced by PBS-g-cellulose nanocrystal (CNC) through melt mixing.
26876864	1	4	theme	dicumyl	200:206	arg1	peroxide					208:215	dicumyl peroxide	200:215	dicumyl peroxide (DCP)	200:221	Poly(butylene succinate) (PBS)/polylactic acid (PLA) blends modified with dicumyl peroxide (DCP) were reinforced by PBS-g-cellulose nanocrystal (CNC) through melt mixing.
26876864	1	4	theme	dicumyl	200:206	arg1	DCP					218:220	DCP	218:220	DCP	218:220	Poly(butylene succinate) (PBS)/polylactic acid (PLA) blends modified with dicumyl peroxide (DCP) were reinforced by PBS-g-cellulose nanocrystal (CNC) through melt mixing.
26876864	5	5	contain	have	1019:1022	arg2	impact					1042:1047	high Notched Izod impact strength and moduli	1024:1067	impact	1042:1047	PBS/PLA blends modified by DCP have high Notched Izod impact strength and moduli, and the values are increased by the addition of PBS-g-CNC.
26876864	5	5	contain	have	1019:1022	arg1	blends					996:1001	PBS/PLA blends	988:1001	PBS/PLA blends modified by DCP	988:1017	PBS/PLA blends modified by DCP have high Notched Izod impact strength and moduli, and the values are increased by the addition of PBS-g-CNC.
26876864	5	5	contain	have	1019:1022	arg2	moduli					1062:1067	moduli	1062:1067	moduli	1062:1067	PBS/PLA blends modified by DCP have high Notched Izod impact strength and moduli, and the values are increased by the addition of PBS-g-CNC.
26876864	1	6	theme	melt	284:287	arg1	mixing					289:294	melt mixing	284:294	melt mixing	284:294	Poly(butylene succinate) (PBS)/polylactic acid (PLA) blends modified with dicumyl peroxide (DCP) were reinforced by PBS-g-cellulose nanocrystal (CNC) through melt mixing.
26876864	3	7	theme	PBS-g-CNC	574:582	arg1	addition					554:561	the addition	550:561	the addition of DCP and PBS-g-CNC	550:582	The morphological analysis of PBS/PLA/PBS-g-CNC composites before and after etched by CH2Cl2 shows that the addition of DCP and PBS-g-CNC could decrease the size of PBS as a dispersed phase in PLA matrix and improve the dispersion of PBS-g-CNC in both PBS and PLA phases, which could affect the crystallization and mechanical properties of composites.
26876864	7	8	theme	PBS/PLA	1413:1419	arg1	composite					1421:1429	PBS/PLA composite	1413:1429	PBS/PLA composite	1413:1429	The addition of DCP decreases the crystallization temperature and crystallinity of PBS/PLA composite, but increases the thermal stability of composites, mostly because of the crosslink effect of DCP on PBS/PLA matrix.
26876864	7	9	theme	composite	1421:1429	arg1	crystallization					1364:1378	the crystallization temperature and crystallinity	1360:1408	crystallization	1364:1378	The addition of DCP decreases the crystallization temperature and crystallinity of PBS/PLA composite, but increases the thermal stability of composites, mostly because of the crosslink effect of DCP on PBS/PLA matrix.
26876864	7	9	theme	composite	1421:1429	arg1	crystallinity					1396:1408	crystallinity	1396:1408	crystallinity	1396:1408	The addition of DCP decreases the crystallization temperature and crystallinity of PBS/PLA composite, but increases the thermal stability of composites, mostly because of the crosslink effect of DCP on PBS/PLA matrix.
26876864	4	10	theme	PBS/PLA/PBS-g-CNC	843:859	arg1	composites					861:870	PBS/PLA/PBS-g-CNC composites	843:870	PBS/PLA/PBS-g-CNC composites	843:870	The crystallinity of PLA α'-phase crystal in PBS/PLA/PBS-g-CNC composites is increased obviously by the addition of PBS-g-CNC, leading to an increase of the crystallinity of the composites.
26876864	5	11	theme	Izod	1037:1040	arg1	impact					1042:1047	high Notched Izod impact strength and moduli	1024:1067	impact	1042:1047	PBS/PLA blends modified by DCP have high Notched Izod impact strength and moduli, and the values are increased by the addition of PBS-g-CNC.
26876864	6	12	theme	translation	1160:1170	arg1	temperature					1172:1182	glass translation temperature	1154:1182	glass translation temperature of PBS/PLA blend	1154:1199	Both storage modulus and glass translation temperature of PBS/PLA blend are increased by DCP and PBS-g-CNC, which is proved by DMA results, showing a weak molecular segment mobility of PBS/PLA matrix.
26876864	3	13	theme	morphological	450:462	arg1	analysis					464:471	The morphological analysis	446:471	The morphological analysis of PBS/PLA/PBS-g-CNC composites before and after etched by CH2Cl2	446:537	The morphological analysis of PBS/PLA/PBS-g-CNC composites before and after etched by CH2Cl2 shows that the addition of DCP and PBS-g-CNC could decrease the size of PBS as a dispersed phase in PLA matrix and improve the dispersion of PBS-g-CNC in both PBS and PLA phases, which could affect the crystallization and mechanical properties of composites.
26876864	3	14	theme	mechanical	761:770	arg1	properties					772:781	mechanical properties	761:781	mechanical properties	761:781	The morphological analysis of PBS/PLA/PBS-g-CNC composites before and after etched by CH2Cl2 shows that the addition of DCP and PBS-g-CNC could decrease the size of PBS as a dispersed phase in PLA matrix and improve the dispersion of PBS-g-CNC in both PBS and PLA phases, which could affect the crystallization and mechanical properties of composites.
26876864	3	15	theme	PLA	706:708	arg1	phases					710:715	both PBS and PLA phases	693:715	phases	710:715	The morphological analysis of PBS/PLA/PBS-g-CNC composites before and after etched by CH2Cl2 shows that the addition of DCP and PBS-g-CNC could decrease the size of PBS as a dispersed phase in PLA matrix and improve the dispersion of PBS-g-CNC in both PBS and PLA phases, which could affect the crystallization and mechanical properties of composites.
26876864	0	16	theme	cellulose	63:71	arg1	nanocrystal					73:83	cellulose nanocrystal	63:83	cellulose nanocrystal on toughened PBS/polylactic acid blends	63:123	Reinforcement effect of poly(butylene succinate) (PBS)-grafted cellulose nanocrystal on toughened PBS/polylactic acid blends.
26876864	7	17	theme	crosslink	1505:1513	arg1	effect					1515:1520	the crosslink effect	1501:1520	the crosslink effect of DCP on PBS/PLA matrix	1501:1545	The addition of DCP decreases the crystallization temperature and crystallinity of PBS/PLA composite, but increases the thermal stability of composites, mostly because of the crosslink effect of DCP on PBS/PLA matrix.
26876864	6	18	theme	glass	1154:1158	arg1	temperature					1172:1182	glass translation temperature	1154:1182	glass translation temperature of PBS/PLA blend	1154:1199	Both storage modulus and glass translation temperature of PBS/PLA blend are increased by DCP and PBS-g-CNC, which is proved by DMA results, showing a weak molecular segment mobility of PBS/PLA matrix.
26876864	7	19	theme	thermal	1450:1456	arg1	stability					1458:1466	the thermal stability	1446:1466	the thermal stability of composites	1446:1480	The addition of DCP decreases the crystallization temperature and crystallinity of PBS/PLA composite, but increases the thermal stability of composites, mostly because of the crosslink effect of DCP on PBS/PLA matrix.
26876864	0	20	theme	toughened	88:96	arg1	blends					118:123	toughened PBS/polylactic acid blends	88:123	toughened PBS/polylactic acid blends	88:123	Reinforcement effect of poly(butylene succinate) (PBS)-grafted cellulose nanocrystal on toughened PBS/polylactic acid blends.
26876864	3	21	dep	crystallization	741:755	arg1	the					737:739	the	737:739	the	737:739	The morphological analysis of PBS/PLA/PBS-g-CNC composites before and after etched by CH2Cl2 shows that the addition of DCP and PBS-g-CNC could decrease the size of PBS as a dispersed phase in PLA matrix and improve the dispersion of PBS-g-CNC in both PBS and PLA phases, which could affect the crystallization and mechanical properties of composites.
26876864	6	22	theme	weak	1279:1282	arg1	mobility					1302:1309	a weak molecular segment mobility	1277:1309	a weak molecular segment mobility of PBS/PLA matrix	1277:1327	Both storage modulus and glass translation temperature of PBS/PLA blend are increased by DCP and PBS-g-CNC, which is proved by DMA results, showing a weak molecular segment mobility of PBS/PLA matrix.
26876864	2	23	theme	GPC	411:413	arg1	analysis					415:422	GPC analysis	411:422	GPC analysis	411:422	PBS-g-CNC was prepared through in situ polymerization and its structure was confirmed by FTIR, (13)C NMR, XPS and GPC analysis after saponification.
26876864	5	24	dep	impact	1042:1047	arg1	strength					1049:1056	strength	1049:1056	strength	1049:1056	PBS/PLA blends modified by DCP have high Notched Izod impact strength and moduli, and the values are increased by the addition of PBS-g-CNC.
26876864	2	25	theme	C	396:396	arg1	NMR					398:400	(13)C NMR	392:400	(13)C NMR	392:400	PBS-g-CNC was prepared through in situ polymerization and its structure was confirmed by FTIR, (13)C NMR, XPS and GPC analysis after saponification.
26876864	3	26	theme	composites	786:795	arg1	crystallization					741:755	crystallization	741:755	crystallization	741:755	The morphological analysis of PBS/PLA/PBS-g-CNC composites before and after etched by CH2Cl2 shows that the addition of DCP and PBS-g-CNC could decrease the size of PBS as a dispersed phase in PLA matrix and improve the dispersion of PBS-g-CNC in both PBS and PLA phases, which could affect the crystallization and mechanical properties of composites.
26876864	3	26	theme	composites	786:795	arg1	properties					772:781	mechanical properties	761:781	mechanical properties	761:781	The morphological analysis of PBS/PLA/PBS-g-CNC composites before and after etched by CH2Cl2 shows that the addition of DCP and PBS-g-CNC could decrease the size of PBS as a dispersed phase in PLA matrix and improve the dispersion of PBS-g-CNC in both PBS and PLA phases, which could affect the crystallization and mechanical properties of composites.
26876864	6	27	theme	PBS/PLA	1314:1320	arg1	matrix					1322:1327	PBS/PLA matrix	1314:1327	PBS/PLA matrix	1314:1327	Both storage modulus and glass translation temperature of PBS/PLA blend are increased by DCP and PBS-g-CNC, which is proved by DMA results, showing a weak molecular segment mobility of PBS/PLA matrix.
26876864	3	28	theme	dispersed	620:628	arg1	size					603:606	the size	599:606	the size of PBS	599:613	The morphological analysis of PBS/PLA/PBS-g-CNC composites before and after etched by CH2Cl2 shows that the addition of DCP and PBS-g-CNC could decrease the size of PBS as a dispersed phase in PLA matrix and improve the dispersion of PBS-g-CNC in both PBS and PLA phases, which could affect the crystallization and mechanical properties of composites.
26876864	3	28	theme	dispersed	620:628	arg1	phase					630:634	a dispersed phase	618:634	a dispersed phase in PLA matrix	618:648	The morphological analysis of PBS/PLA/PBS-g-CNC composites before and after etched by CH2Cl2 shows that the addition of DCP and PBS-g-CNC could decrease the size of PBS as a dispersed phase in PLA matrix and improve the dispersion of PBS-g-CNC in both PBS and PLA phases, which could affect the crystallization and mechanical properties of composites.
26876864	6	29	theme	storage	1134:1140	arg1	modulus					1142:1148	storage modulus	1134:1148	storage modulus	1134:1148	Both storage modulus and glass translation temperature of PBS/PLA blend are increased by DCP and PBS-g-CNC, which is proved by DMA results, showing a weak molecular segment mobility of PBS/PLA matrix.
26876864	3	30	theme	composites	494:503	arg1	analysis					464:471	The morphological analysis	446:471	The morphological analysis of PBS/PLA/PBS-g-CNC composites before and after etched by CH2Cl2	446:537	The morphological analysis of PBS/PLA/PBS-g-CNC composites before and after etched by CH2Cl2 shows that the addition of DCP and PBS-g-CNC could decrease the size of PBS as a dispersed phase in PLA matrix and improve the dispersion of PBS-g-CNC in both PBS and PLA phases, which could affect the crystallization and mechanical properties of composites.
26876864	2	31	theme	in	328:329	arg1	polymerization					336:349	in situ polymerization	328:349	in situ polymerization	328:349	PBS-g-CNC was prepared through in situ polymerization and its structure was confirmed by FTIR, (13)C NMR, XPS and GPC analysis after saponification.
26876864	0	32	theme	Reinforcement	0:12	arg1	effect					14:19	Reinforcement effect	0:19	Reinforcement effect of poly(butylene succinate) (PBS)	0:53	Reinforcement effect of poly(butylene succinate) (PBS)-grafted cellulose nanocrystal on toughened PBS/polylactic acid blends.
26876864	6	33	theme	molecular	1284:1292	arg1	mobility					1302:1309	a weak molecular segment mobility	1277:1309	a weak molecular segment mobility of PBS/PLA matrix	1277:1327	Both storage modulus and glass translation temperature of PBS/PLA blend are increased by DCP and PBS-g-CNC, which is proved by DMA results, showing a weak molecular segment mobility of PBS/PLA matrix.
26876864	0	34	theme	poly	24:27	arg1	effect					14:19	Reinforcement effect	0:19	Reinforcement effect of poly(butylene succinate) (PBS)	0:53	Reinforcement effect of poly(butylene succinate) (PBS)-grafted cellulose nanocrystal on toughened PBS/polylactic acid blends.
26876864	7	35	theme	DCP	1525:1527	arg1	effect					1515:1520	the crosslink effect	1501:1520	the crosslink effect of DCP on PBS/PLA matrix	1501:1545	The addition of DCP decreases the crystallization temperature and crystallinity of PBS/PLA composite, but increases the thermal stability of composites, mostly because of the crosslink effect of DCP on PBS/PLA matrix.
26876864	0	36	theme	acid	113:116	arg1	blends					118:123	toughened PBS/polylactic acid blends	88:123	toughened PBS/polylactic acid blends	88:123	Reinforcement effect of poly(butylene succinate) (PBS)-grafted cellulose nanocrystal on toughened PBS/polylactic acid blends.
26876864	1	37	theme	/polylactic	156:166	arg1	blends					179:184	Poly(butylene succinate) (PBS)/polylactic acid (PLA) blends	126:184	Poly(butylene succinate) (PBS)/polylactic acid (PLA) blends modified with dicumyl peroxide (DCP)	126:221	Poly(butylene succinate) (PBS)/polylactic acid (PLA) blends modified with dicumyl peroxide (DCP) were reinforced by PBS-g-cellulose nanocrystal (CNC) through melt mixing.
26876864	6	38	theme	segment	1294:1300	arg1	mobility					1302:1309	a weak molecular segment mobility	1277:1309	a weak molecular segment mobility of PBS/PLA matrix	1277:1327	Both storage modulus and glass translation temperature of PBS/PLA blend are increased by DCP and PBS-g-CNC, which is proved by DMA results, showing a weak molecular segment mobility of PBS/PLA matrix.
26876864	3	39	theme	PLA	639:641	arg1	matrix					643:648	PLA matrix	639:648	PLA matrix	639:648	The morphological analysis of PBS/PLA/PBS-g-CNC composites before and after etched by CH2Cl2 shows that the addition of DCP and PBS-g-CNC could decrease the size of PBS as a dispersed phase in PLA matrix and improve the dispersion of PBS-g-CNC in both PBS and PLA phases, which could affect the crystallization and mechanical properties of composites.
26876864	0	40	theme	PBS/polylactic	98:111	arg1	blends					118:123	toughened PBS/polylactic acid blends	88:123	toughened PBS/polylactic acid blends	88:123	Reinforcement effect of poly(butylene succinate) (PBS)-grafted cellulose nanocrystal on toughened PBS/polylactic acid blends.
26876864	3	41	theme	DCP	566:568	arg1	addition					554:561	the addition	550:561	the addition of DCP and PBS-g-CNC	550:582	The morphological analysis of PBS/PLA/PBS-g-CNC composites before and after etched by CH2Cl2 shows that the addition of DCP and PBS-g-CNC could decrease the size of PBS as a dispersed phase in PLA matrix and improve the dispersion of PBS-g-CNC in both PBS and PLA phases, which could affect the crystallization and mechanical properties of composites.
26876864	3	42	theme	PBS/PLA/PBS-g-CNC	476:492	arg1	composites					494:503	PBS/PLA/PBS-g-CNC composites	476:503	PBS/PLA/PBS-g-CNC composites before and after etched	476:527	The morphological analysis of PBS/PLA/PBS-g-CNC composites before and after etched by CH2Cl2 shows that the addition of DCP and PBS-g-CNC could decrease the size of PBS as a dispersed phase in PLA matrix and improve the dispersion of PBS-g-CNC in both PBS and PLA phases, which could affect the crystallization and mechanical properties of composites.
26876864	1	43	theme	acid	168:171	arg1	blends					179:184	Poly(butylene succinate) (PBS)/polylactic acid (PLA) blends	126:184	Poly(butylene succinate) (PBS)/polylactic acid (PLA) blends modified with dicumyl peroxide (DCP)	126:221	Poly(butylene succinate) (PBS)/polylactic acid (PLA) blends modified with dicumyl peroxide (DCP) were reinforced by PBS-g-cellulose nanocrystal (CNC) through melt mixing.
26876864	0	44	theme	butylene	29:36	arg1	poly					24:27	poly	24:27	poly(butylene succinate) (PBS)	24:53	Reinforcement effect of poly(butylene succinate) (PBS)-grafted cellulose nanocrystal on toughened PBS/polylactic acid blends.
26876864	0	44	theme	butylene	29:36	arg1	succinate					38:46	butylene succinate	29:46	butylene succinate	29:46	Reinforcement effect of poly(butylene succinate) (PBS)-grafted cellulose nanocrystal on toughened PBS/polylactic acid blends.
26876864	4	45	theme	crystal	832:838	arg1	crystallinity					802:814	The crystallinity	798:814	The crystallinity of PLA α'-phase crystal in PBS/PLA/PBS-g-CNC composites	798:870	The crystallinity of PLA α'-phase crystal in PBS/PLA/PBS-g-CNC composites is increased obviously by the addition of PBS-g-CNC, leading to an increase of the crystallinity of the composites.
26876864	7	46	theme	PBS/PLA	1532:1538	arg1	matrix					1540:1545	PBS/PLA matrix	1532:1545	PBS/PLA matrix	1532:1545	The addition of DCP decreases the crystallization temperature and crystallinity of PBS/PLA composite, but increases the thermal stability of composites, mostly because of the crosslink effect of DCP on PBS/PLA matrix.
26876864	6	47	theme	matrix	1322:1327	arg1	mobility					1302:1309	a weak molecular segment mobility	1277:1309	a weak molecular segment mobility of PBS/PLA matrix	1277:1327	Both storage modulus and glass translation temperature of PBS/PLA blend are increased by DCP and PBS-g-CNC, which is proved by DMA results, showing a weak molecular segment mobility of PBS/PLA matrix.
26876864	1	48	theme	PBS-g-cellulose	242:256	arg1	nanocrystal					258:268	PBS-g-cellulose nanocrystal	242:268	PBS-g-cellulose nanocrystal (CNC)	242:274	Poly(butylene succinate) (PBS)/polylactic acid (PLA) blends modified with dicumyl peroxide (DCP) were reinforced by PBS-g-cellulose nanocrystal (CNC) through melt mixing.
26876864	1	48	theme	PBS-g-cellulose	242:256	arg1	CNC					271:273	CNC	271:273	CNC	271:273	Poly(butylene succinate) (PBS)/polylactic acid (PLA) blends modified with dicumyl peroxide (DCP) were reinforced by PBS-g-cellulose nanocrystal (CNC) through melt mixing.
26876864	4	49	theme	α'-phase	823:830	arg1	crystal					832:838	PLA α'-phase crystal	819:838	PLA α'-phase crystal	819:838	The crystallinity of PLA α'-phase crystal in PBS/PLA/PBS-g-CNC composites is increased obviously by the addition of PBS-g-CNC, leading to an increase of the crystallinity of the composites.
26876864	3	50	from	phase	630:634	arg1	matrix					643:648	PLA matrix	639:648	PLA matrix	639:648	The morphological analysis of PBS/PLA/PBS-g-CNC composites before and after etched by CH2Cl2 shows that the addition of DCP and PBS-g-CNC could decrease the size of PBS as a dispersed phase in PLA matrix and improve the dispersion of PBS-g-CNC in both PBS and PLA phases, which could affect the crystallization and mechanical properties of composites.
26876864	1	51	theme	PLA	174:176	arg1	blends					179:184	Poly(butylene succinate) (PBS)/polylactic acid (PLA) blends	126:184	Poly(butylene succinate) (PBS)/polylactic acid (PLA) blends modified with dicumyl peroxide (DCP)	126:221	Poly(butylene succinate) (PBS)/polylactic acid (PLA) blends modified with dicumyl peroxide (DCP) were reinforced by PBS-g-cellulose nanocrystal (CNC) through melt mixing.
26876864	3	52	theme	PBS-g-CNC	680:688	arg1	dispersion					666:675	the dispersion	662:675	the dispersion of PBS-g-CNC	662:688	The morphological analysis of PBS/PLA/PBS-g-CNC composites before and after etched by CH2Cl2 shows that the addition of DCP and PBS-g-CNC could decrease the size of PBS as a dispersed phase in PLA matrix and improve the dispersion of PBS-g-CNC in both PBS and PLA phases, which could affect the crystallization and mechanical properties of composites.
26876864	5	53	theme	PBS/PLA	988:994	arg1	blends					996:1001	PBS/PLA blends	988:1001	PBS/PLA blends modified by DCP	988:1017	PBS/PLA blends modified by DCP have high Notched Izod impact strength and moduli, and the values are increased by the addition of PBS-g-CNC.
26876864	5	54	theme	Notched	1029:1035	arg1	impact					1042:1047	high Notched Izod impact strength and moduli	1024:1067	impact	1042:1047	PBS/PLA blends modified by DCP have high Notched Izod impact strength and moduli, and the values are increased by the addition of PBS-g-CNC.
26876864	4	55	theme	PBS-g-CNC	914:922	arg1	addition					902:909	the addition	898:909	the addition of PBS-g-CNC	898:922	The crystallinity of PLA α'-phase crystal in PBS/PLA/PBS-g-CNC composites is increased obviously by the addition of PBS-g-CNC, leading to an increase of the crystallinity of the composites.
26876864	4	56	theme	PLA	819:821	arg1	crystal					832:838	PLA α'-phase crystal	819:838	PLA α'-phase crystal	819:838	The crystallinity of PLA α'-phase crystal in PBS/PLA/PBS-g-CNC composites is increased obviously by the addition of PBS-g-CNC, leading to an increase of the crystallinity of the composites.
26876864	4	57	from	crystallinity	802:814	arg1	composites					861:870	PBS/PLA/PBS-g-CNC composites	843:870	PBS/PLA/PBS-g-CNC composites	843:870	The crystallinity of PLA α'-phase crystal in PBS/PLA/PBS-g-CNC composites is increased obviously by the addition of PBS-g-CNC, leading to an increase of the crystallinity of the composites.
26876864	7	58	from	effect	1515:1520	arg1	matrix					1540:1545	PBS/PLA matrix	1532:1545	PBS/PLA matrix	1532:1545	The addition of DCP decreases the crystallization temperature and crystallinity of PBS/PLA composite, but increases the thermal stability of composites, mostly because of the crosslink effect of DCP on PBS/PLA matrix.
26876864	4	59	theme	composites	976:985	arg1	crystallinity					955:967	the crystallinity	951:967	the crystallinity of the composites	951:985	The crystallinity of PLA α'-phase crystal in PBS/PLA/PBS-g-CNC composites is increased obviously by the addition of PBS-g-CNC, leading to an increase of the crystallinity of the composites.
26876864	6	60	theme	blend	1195:1199	arg1	modulus					1142:1148	storage modulus	1134:1148	storage modulus	1134:1148	Both storage modulus and glass translation temperature of PBS/PLA blend are increased by DCP and PBS-g-CNC, which is proved by DMA results, showing a weak molecular segment mobility of PBS/PLA matrix.
26876864	6	60	theme	blend	1195:1199	arg1	temperature					1172:1182	glass translation temperature	1154:1182	glass translation temperature of PBS/PLA blend	1154:1199	Both storage modulus and glass translation temperature of PBS/PLA blend are increased by DCP and PBS-g-CNC, which is proved by DMA results, showing a weak molecular segment mobility of PBS/PLA matrix.
26876864	2	61	dep	in	328:329	arg1	situ					331:334	situ	331:334	situ	331:334	PBS-g-CNC was prepared through in situ polymerization and its structure was confirmed by FTIR, (13)C NMR, XPS and GPC analysis after saponification.
26876864	7	62	theme	DCP	1346:1348	arg1	addition					1334:1341	The addition	1330:1341	The addition of DCP	1330:1348	The addition of DCP decreases the crystallization temperature and crystallinity of PBS/PLA composite, but increases the thermal stability of composites, mostly because of the crosslink effect of DCP on PBS/PLA matrix.
26876864	3	63	theme	PBS	698:700	arg1	phases					710:715	both PBS and PLA phases	693:715	phases	710:715	The morphological analysis of PBS/PLA/PBS-g-CNC composites before and after etched by CH2Cl2 shows that the addition of DCP and PBS-g-CNC could decrease the size of PBS as a dispersed phase in PLA matrix and improve the dispersion of PBS-g-CNC in both PBS and PLA phases, which could affect the crystallization and mechanical properties of composites.
26876864	6	64	theme	PBS/PLA	1187:1193	arg1	blend					1195:1199	PBS/PLA blend	1187:1199	PBS/PLA blend	1187:1199	Both storage modulus and glass translation temperature of PBS/PLA blend are increased by DCP and PBS-g-CNC, which is proved by DMA results, showing a weak molecular segment mobility of PBS/PLA matrix.
26876864	0	65	from	nanocrystal	73:83	arg1	blends					118:123	toughened PBS/polylactic acid blends	88:123	toughened PBS/polylactic acid blends	88:123	Reinforcement effect of poly(butylene succinate) (PBS)-grafted cellulose nanocrystal on toughened PBS/polylactic acid blends.
26876864	4	66	theme	crystallinity	955:967	arg1	increase					939:946	an increase	936:946	an increase of the crystallinity of the composites	936:985	The crystallinity of PLA α'-phase crystal in PBS/PLA/PBS-g-CNC composites is increased obviously by the addition of PBS-g-CNC, leading to an increase of the crystallinity of the composites.
26876864	1	67	theme	Poly	126:129	arg1	blends					179:184	Poly(butylene succinate) (PBS)/polylactic acid (PLA) blends	126:184	Poly(butylene succinate) (PBS)/polylactic acid (PLA) blends modified with dicumyl peroxide (DCP)	126:221	Poly(butylene succinate) (PBS)/polylactic acid (PLA) blends modified with dicumyl peroxide (DCP) were reinforced by PBS-g-cellulose nanocrystal (CNC) through melt mixing.
26876864	5	68	theme	PBS-g-CNC	1118:1126	arg1	addition					1106:1113	the addition	1102:1113	the addition of PBS-g-CNC	1102:1126	PBS/PLA blends modified by DCP have high Notched Izod impact strength and moduli, and the values are increased by the addition of PBS-g-CNC.
26876864	3	69	theme	PBS	611:613	arg1	size					603:606	the size	599:606	the size of PBS	599:613	The morphological analysis of PBS/PLA/PBS-g-CNC composites before and after etched by CH2Cl2 shows that the addition of DCP and PBS-g-CNC could decrease the size of PBS as a dispersed phase in PLA matrix and improve the dispersion of PBS-g-CNC in both PBS and PLA phases, which could affect the crystallization and mechanical properties of composites.
26876864	3	69	theme	PBS	611:613	arg1	phase					630:634	a dispersed phase	618:634	a dispersed phase in PLA matrix	618:648	The morphological analysis of PBS/PLA/PBS-g-CNC composites before and after etched by CH2Cl2 shows that the addition of DCP and PBS-g-CNC could decrease the size of PBS as a dispersed phase in PLA matrix and improve the dispersion of PBS-g-CNC in both PBS and PLA phases, which could affect the crystallization and mechanical properties of composites.
26876864	7	70	theme	composites	1471:1480	arg1	stability					1458:1466	the thermal stability	1446:1466	the thermal stability of composites	1446:1480	The addition of DCP decreases the crystallization temperature and crystallinity of PBS/PLA composite, but increases the thermal stability of composites, mostly because of the crosslink effect of DCP on PBS/PLA matrix.
26290580	5	0	theme	cell	1163:1166	arg1	lysates					1168:1174	Chlamydia-infected HeLa cell lysates	1139:1174	Chlamydia-infected HeLa cell lysates	1139:1174	We designed a new method of muropeptide isolation consisting of a double filtration step coupled with reverse-phase HPLC fractionation of Chlamydia-infected HeLa cell lysates.
26290580	4	1	theme	C.	882:883	arg1	lysates					911:917	C. trachomatis-infected cell lysates	882:917	C. trachomatis-infected cell lysates	882:917	We used the NOD2-dependent activation of NF-κB by C. trachomatis-infected cell lysates as a biomarker for the presence of PG fragments within specific lysate fractions.
26290580	2	2	from	PG	424:425	arg1	trachomatis					440:450	Chlamydia trachomatis	430:450	Chlamydia trachomatis	430:450	We recently detected PG in Chlamydia trachomatis by a new metabolic cell wall labeling method, however efforts to purify PG from pathogenic Chlamydiae have remained unsuccessful.
26290580	0	3	from	peptidoglycan	71:83	arg1	muropeptides					31:42	muropeptides	31:42	muropeptides from Chlamydia trachomatis peptidoglycan	31:83	Structural characterization of muropeptides from Chlamydia trachomatis peptidoglycan by mass spectrometry resolves "chlamydial anomaly".
26290580	0	3	from	peptidoglycan	71:83	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization of muropeptides from Chlamydia trachomatis peptidoglycan by mass spectrometry	0:104	Structural characterization of muropeptides from Chlamydia trachomatis peptidoglycan by mass spectrometry resolves "chlamydial anomaly".
26290580	6	4	theme	NOD2	1202:1205	arg1	activity					1207:1214	NOD2 activity	1202:1214	NOD2 activity	1202:1214	Fractions that displayed NOD2 activity were analyzed by electrospray ionization mass spectrometry, confirming the presence of muramyl di- and tripeptides in Chlamydia-infected cell lysate fractions.
26290580	2	5	theme	labeling	481:488	arg1	method					490:495	a new metabolic cell wall labeling method	455:495	a new metabolic cell wall labeling method	455:495	We recently detected PG in Chlamydia trachomatis by a new metabolic cell wall labeling method, however efforts to purify PG from pathogenic Chlamydiae have remained unsuccessful.
26290580	0	6	from	characterization	11:26	arg1	peptidoglycan					71:83	Chlamydia trachomatis peptidoglycan	49:83	Chlamydia trachomatis peptidoglycan	49:83	Structural characterization of muropeptides from Chlamydia trachomatis peptidoglycan by mass spectrometry resolves "chlamydial anomaly".
26290580	5	7	theme	double	1067:1072	arg1	step					1085:1088	a double filtration step	1065:1088	a double filtration step coupled with reverse-phase HPLC fractionation of Chlamydia-infected HeLa cell lysates	1065:1174	We designed a new method of muropeptide isolation consisting of a double filtration step coupled with reverse-phase HPLC fractionation of Chlamydia-infected HeLa cell lysates.
26290580	6	8	theme	di-	1311:1313	arg1	presence					1291:1298	the presence	1287:1298	the presence of muramyl di- and tripeptides in Chlamydia-infected cell lysate fractions	1287:1373	Fractions that displayed NOD2 activity were analyzed by electrospray ionization mass spectrometry, confirming the presence of muramyl di- and tripeptides in Chlamydia-infected cell lysate fractions.
26290580	5	9	theme	reverse-phase	1103:1115	arg1	fractionation					1122:1134	reverse-phase HPLC fractionation	1103:1134	reverse-phase HPLC fractionation of Chlamydia-infected HeLa cell lysates	1103:1174	We designed a new method of muropeptide isolation consisting of a double filtration step coupled with reverse-phase HPLC fractionation of Chlamydia-infected HeLa cell lysates.
26290580	7	10	theme	mass	1390:1393	arg1	spectrometry					1395:1406	the mass spectrometry data	1386:1411	the mass spectrometry data of large muropeptide fragments	1386:1442	Moreover, the mass spectrometry data of large muropeptide fragments provided evidence that transpeptidation and transglycosylation reactions occur in pathogenic Chlamydiae.
26290580	6	11	theme	cell	1353:1356	arg1	fractions					1365:1373	Chlamydia-infected cell lysate fractions	1334:1373	Chlamydia-infected cell lysate fractions	1334:1373	Fractions that displayed NOD2 activity were analyzed by electrospray ionization mass spectrometry, confirming the presence of muramyl di- and tripeptides in Chlamydia-infected cell lysate fractions.
26290580	0	12	theme	chlamydial	116:125	arg1	"					134:134	chlamydial anomaly"	116:134	"chlamydial anomaly"	115:134	Structural characterization of muropeptides from Chlamydia trachomatis peptidoglycan by mass spectrometry resolves "chlamydial anomaly".
26290580	1	13	theme	antibiotic	344:353	arg1	data					370:373	antibiotic susceptibility data	344:373	antibiotic susceptibility data that suggest its existence	344:400	The "chlamydial anomaly," first coined by James Moulder, describes the inability of researchers to detect or purify peptidoglycan (PG) from pathogenic Chlamydiae despite genetic and biochemical evidence and antibiotic susceptibility data that suggest its existence.
26290580	4	14	theme	cell	906:909	arg1	lysates					911:917	C. trachomatis-infected cell lysates	882:917	C. trachomatis-infected cell lysates	882:917	We used the NOD2-dependent activation of NF-κB by C. trachomatis-infected cell lysates as a biomarker for the presence of PG fragments within specific lysate fractions.
26290580	3	15	theme	chlamydial	593:602	arg1	species					604:610	Pathogenic chlamydial species	582:610	Pathogenic chlamydial species	582:610	Pathogenic chlamydial species are known to activate nucleotide-binding oligomerization domain-containing protein 2 (NOD2) innate immune receptors by as yet uncharacterized ligands, which are presumed to be PG fragments (muramyl di- and tripeptides).
26290580	7	16	theme	pathogenic	1526:1535	arg1	Chlamydiae					1537:1546	pathogenic Chlamydiae	1526:1546	pathogenic Chlamydiae	1526:1546	Moreover, the mass spectrometry data of large muropeptide fragments provided evidence that transpeptidation and transglycosylation reactions occur in pathogenic Chlamydiae.
26290580	4	17	theme	PG	954:955	arg1	fragments					957:965	PG fragments	954:965	PG fragments within specific lysate fractions	954:998	We used the NOD2-dependent activation of NF-κB by C. trachomatis-infected cell lysates as a biomarker for the presence of PG fragments within specific lysate fractions.
26290580	1	18	theme	susceptibility	355:368	arg1	data					370:373	antibiotic susceptibility data	344:373	antibiotic susceptibility data that suggest its existence	344:400	The "chlamydial anomaly," first coined by James Moulder, describes the inability of researchers to detect or purify peptidoglycan (PG) from pathogenic Chlamydiae despite genetic and biochemical evidence and antibiotic susceptibility data that suggest its existence.
26290580	4	19	theme	trachomatis-infected	885:904	arg1	lysates					911:917	C. trachomatis-infected cell lysates	882:917	C. trachomatis-infected cell lysates	882:917	We used the NOD2-dependent activation of NF-κB by C. trachomatis-infected cell lysates as a biomarker for the presence of PG fragments within specific lysate fractions.
26290580	6	20	theme	tripeptides	1319:1329	arg1	presence					1291:1298	the presence	1287:1298	the presence of muramyl di- and tripeptides in Chlamydia-infected cell lysate fractions	1287:1373	Fractions that displayed NOD2 activity were analyzed by electrospray ionization mass spectrometry, confirming the presence of muramyl di- and tripeptides in Chlamydia-infected cell lysate fractions.
26290580	4	21	used	used	835:838	arg2	We					832:833	We	832:833	We	832:833	We used the NOD2-dependent activation of NF-κB by C. trachomatis-infected cell lysates as a biomarker for the presence of PG fragments within specific lysate fractions.
26290580	7	22	theme	transglycosylation	1488:1505	arg1	reactions					1507:1515	transpeptidation and transglycosylation reactions	1467:1515	reactions	1507:1515	Moreover, the mass spectrometry data of large muropeptide fragments provided evidence that transpeptidation and transglycosylation reactions occur in pathogenic Chlamydiae.
26290580	4	23	theme	specific	974:981	arg1	fractions					990:998	specific lysate fractions	974:998	specific lysate fractions	974:998	We used the NOD2-dependent activation of NF-κB by C. trachomatis-infected cell lysates as a biomarker for the presence of PG fragments within specific lysate fractions.
26290580	3	24	dep	fragments	791:799	arg1	tripeptides					818:828	tripeptides	818:828	tripeptides	818:828	Pathogenic chlamydial species are known to activate nucleotide-binding oligomerization domain-containing protein 2 (NOD2) innate immune receptors by as yet uncharacterized ligands, which are presumed to be PG fragments (muramyl di- and tripeptides).
26290580	3	24	dep	fragments	791:799	arg1	di-					810:812	muramyl di-	802:812	muramyl di-	802:812	Pathogenic chlamydial species are known to activate nucleotide-binding oligomerization domain-containing protein 2 (NOD2) innate immune receptors by as yet uncharacterized ligands, which are presumed to be PG fragments (muramyl di- and tripeptides).
26290580	7	25	theme	fragments	1434:1442	arg1	spectrometry					1395:1406	the mass spectrometry data	1386:1411	the mass spectrometry data of large muropeptide fragments	1386:1442	Moreover, the mass spectrometry data of large muropeptide fragments provided evidence that transpeptidation and transglycosylation reactions occur in pathogenic Chlamydiae.
26290580	6	26	theme	mass	1257:1260	arg1	spectrometry					1262:1273	electrospray ionization mass spectrometry	1233:1273	electrospray ionization mass spectrometry	1233:1273	Fractions that displayed NOD2 activity were analyzed by electrospray ionization mass spectrometry, confirming the presence of muramyl di- and tripeptides in Chlamydia-infected cell lysate fractions.
26290580	0	27	theme	anomaly	127:133	arg1	"					134:134	chlamydial anomaly"	116:134	"chlamydial anomaly"	115:134	Structural characterization of muropeptides from Chlamydia trachomatis peptidoglycan by mass spectrometry resolves "chlamydial anomaly".
26290580	3	28	theme	uncharacterized	738:752	arg1	ligands					754:760	as yet uncharacterized ligands	731:760	as yet uncharacterized ligands	731:760	Pathogenic chlamydial species are known to activate nucleotide-binding oligomerization domain-containing protein 2 (NOD2) innate immune receptors by as yet uncharacterized ligands, which are presumed to be PG fragments (muramyl di- and tripeptides).
26290580	2	29	theme	wall	476:479	arg1	method					490:495	a new metabolic cell wall labeling method	455:495	a new metabolic cell wall labeling method	455:495	We recently detected PG in Chlamydia trachomatis by a new metabolic cell wall labeling method, however efforts to purify PG from pathogenic Chlamydiae have remained unsuccessful.
26290580	8	30	theme	glycanless	1621:1630	arg1	hypothesis					1647:1656	the "glycanless peptidoglycan" hypothesis	1616:1656	the "glycanless peptidoglycan" hypothesis	1616:1656	These results reveal the composition of chlamydial PG and disprove the "glycanless peptidoglycan" hypothesis.
26290580	3	31	theme	PG	788:789	arg1	fragments					791:799	PG fragments	788:799	PG fragments (muramyl di- and tripeptides)	788:829	Pathogenic chlamydial species are known to activate nucleotide-binding oligomerization domain-containing protein 2 (NOD2) innate immune receptors by as yet uncharacterized ligands, which are presumed to be PG fragments (muramyl di- and tripeptides).
26290580	5	32	theme	Chlamydia-infected	1139:1156	arg1	lysates					1168:1174	Chlamydia-infected HeLa cell lysates	1139:1174	Chlamydia-infected HeLa cell lysates	1139:1174	We designed a new method of muropeptide isolation consisting of a double filtration step coupled with reverse-phase HPLC fractionation of Chlamydia-infected HeLa cell lysates.
26290580	4	33	theme	NF-κB	873:877	arg1	biomarker					924:932	a biomarker	922:932	a biomarker for the presence of PG fragments within specific lysate fractions	922:998	We used the NOD2-dependent activation of NF-κB by C. trachomatis-infected cell lysates as a biomarker for the presence of PG fragments within specific lysate fractions.
26290580	4	33	theme	NF-κB	873:877	arg1	activation					859:868	the NOD2-dependent activation	840:868	the NOD2-dependent activation of NF-κB by C. trachomatis-infected cell lysates	840:917	We used the NOD2-dependent activation of NF-κB by C. trachomatis-infected cell lysates as a biomarker for the presence of PG fragments within specific lysate fractions.
26290580	4	34	theme	fragments	957:965	arg1	presence					942:949	the presence	938:949	the presence of PG fragments within specific lysate fractions	938:998	We used the NOD2-dependent activation of NF-κB by C. trachomatis-infected cell lysates as a biomarker for the presence of PG fragments within specific lysate fractions.
26290580	0	35	theme	Structural	0:9	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization of muropeptides from Chlamydia trachomatis peptidoglycan by mass spectrometry	0:104	Structural characterization of muropeptides from Chlamydia trachomatis peptidoglycan by mass spectrometry resolves "chlamydial anomaly".
26290580	3	36	theme	Pathogenic	582:591	arg1	species					604:610	Pathogenic chlamydial species	582:610	Pathogenic chlamydial species	582:610	Pathogenic chlamydial species are known to activate nucleotide-binding oligomerization domain-containing protein 2 (NOD2) innate immune receptors by as yet uncharacterized ligands, which are presumed to be PG fragments (muramyl di- and tripeptides).
26290580	6	37	theme	Chlamydia-infected	1334:1351	arg1	fractions					1365:1373	Chlamydia-infected cell lysate fractions	1334:1373	Chlamydia-infected cell lysate fractions	1334:1373	Fractions that displayed NOD2 activity were analyzed by electrospray ionization mass spectrometry, confirming the presence of muramyl di- and tripeptides in Chlamydia-infected cell lysate fractions.
26290580	0	38	theme	muropeptides	31:42	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization of muropeptides from Chlamydia trachomatis peptidoglycan by mass spectrometry	0:104	Structural characterization of muropeptides from Chlamydia trachomatis peptidoglycan by mass spectrometry resolves "chlamydial anomaly".
26290580	6	39	theme	ionization	1246:1255	arg1	spectrometry					1262:1273	electrospray ionization mass spectrometry	1233:1273	electrospray ionization mass spectrometry	1233:1273	Fractions that displayed NOD2 activity were analyzed by electrospray ionization mass spectrometry, confirming the presence of muramyl di- and tripeptides in Chlamydia-infected cell lysate fractions.
26290580	7	40	dep	spectrometry	1395:1406	arg1	data					1408:1411	data	1408:1411	the mass spectrometry data of large muropeptide fragments	1386:1442	Moreover, the mass spectrometry data of large muropeptide fragments provided evidence that transpeptidation and transglycosylation reactions occur in pathogenic Chlamydiae.
26290580	7	41	theme	transpeptidation	1467:1482	arg1	reactions					1507:1515	transpeptidation and transglycosylation reactions	1467:1515	reactions	1507:1515	Moreover, the mass spectrometry data of large muropeptide fragments provided evidence that transpeptidation and transglycosylation reactions occur in pathogenic Chlamydiae.
26290580	2	42	theme	Chlamydia	430:438	arg1	trachomatis					440:450	Chlamydia trachomatis	430:450	Chlamydia trachomatis	430:450	We recently detected PG in Chlamydia trachomatis by a new metabolic cell wall labeling method, however efforts to purify PG from pathogenic Chlamydiae have remained unsuccessful.
26290580	2	43	dep	efforts	506:512	arg1	purify					517:522	purify	517:522	to purify PG from pathogenic Chlamydiae	514:552	We recently detected PG in Chlamydia trachomatis by a new metabolic cell wall labeling method, however efforts to purify PG from pathogenic Chlamydiae have remained unsuccessful.
26290580	5	44	theme	isolation	1041:1049	arg1	method					1019:1024	a new method	1013:1024	a new method of muropeptide isolation consisting of a double filtration step coupled with reverse-phase HPLC fractionation of Chlamydia-infected HeLa cell lysates	1013:1174	We designed a new method of muropeptide isolation consisting of a double filtration step coupled with reverse-phase HPLC fractionation of Chlamydia-infected HeLa cell lysates.
26290580	7	45	theme	muropeptide	1422:1432	arg1	fragments					1434:1442	large muropeptide fragments	1416:1442	large muropeptide fragments	1416:1442	Moreover, the mass spectrometry data of large muropeptide fragments provided evidence that transpeptidation and transglycosylation reactions occur in pathogenic Chlamydiae.
26290580	0	46	theme	Chlamydia	49:57	arg1	peptidoglycan					71:83	Chlamydia trachomatis peptidoglycan	49:83	Chlamydia trachomatis peptidoglycan	49:83	Structural characterization of muropeptides from Chlamydia trachomatis peptidoglycan by mass spectrometry resolves "chlamydial anomaly".
26290580	1	47	theme	pathogenic	277:286	arg1	Chlamydiae					288:297	pathogenic Chlamydiae	277:297	pathogenic Chlamydiae	277:297	The "chlamydial anomaly," first coined by James Moulder, describes the inability of researchers to detect or purify peptidoglycan (PG) from pathogenic Chlamydiae despite genetic and biochemical evidence and antibiotic susceptibility data that suggest its existence.
26290580	2	48	theme	pathogenic	532:541	arg1	Chlamydiae					543:552	pathogenic Chlamydiae	532:552	pathogenic Chlamydiae	532:552	We recently detected PG in Chlamydia trachomatis by a new metabolic cell wall labeling method, however efforts to purify PG from pathogenic Chlamydiae have remained unsuccessful.
26290580	7	49	theme	large	1416:1420	arg1	fragments					1434:1442	large muropeptide fragments	1416:1442	large muropeptide fragments	1416:1442	Moreover, the mass spectrometry data of large muropeptide fragments provided evidence that transpeptidation and transglycosylation reactions occur in pathogenic Chlamydiae.
26290580	2	50	theme	cell	471:474	arg1	method					490:495	a new metabolic cell wall labeling method	455:495	a new metabolic cell wall labeling method	455:495	We recently detected PG in Chlamydia trachomatis by a new metabolic cell wall labeling method, however efforts to purify PG from pathogenic Chlamydiae have remained unsuccessful.
26290580	3	51	theme	domain-containing	669:685	arg1	receptors					718:726	nucleotide-binding oligomerization domain-containing protein 2 (NOD2) innate immune receptors	634:726	nucleotide-binding oligomerization domain-containing protein 2 (NOD2) innate immune receptors	634:726	Pathogenic chlamydial species are known to activate nucleotide-binding oligomerization domain-containing protein 2 (NOD2) innate immune receptors by as yet uncharacterized ligands, which are presumed to be PG fragments (muramyl di- and tripeptides).
26290580	5	52	theme	muropeptide	1029:1039	arg1	isolation					1041:1049	muropeptide isolation	1029:1049	muropeptide isolation consisting of a double filtration step coupled with reverse-phase HPLC fractionation of Chlamydia-infected HeLa cell lysates	1029:1174	We designed a new method of muropeptide isolation consisting of a double filtration step coupled with reverse-phase HPLC fractionation of Chlamydia-infected HeLa cell lysates.
26290580	2	53	theme	metabolic	461:469	arg1	method					490:495	a new metabolic cell wall labeling method	455:495	a new metabolic cell wall labeling method	455:495	We recently detected PG in Chlamydia trachomatis by a new metabolic cell wall labeling method, however efforts to purify PG from pathogenic Chlamydiae have remained unsuccessful.
26290580	3	54	theme	muramyl	802:808	arg1	di-					810:812	muramyl di-	802:812	muramyl di-	802:812	Pathogenic chlamydial species are known to activate nucleotide-binding oligomerization domain-containing protein 2 (NOD2) innate immune receptors by as yet uncharacterized ligands, which are presumed to be PG fragments (muramyl di- and tripeptides).
26290580	5	55	theme	HPLC	1117:1120	arg1	fractionation					1122:1134	reverse-phase HPLC fractionation	1103:1134	reverse-phase HPLC fractionation of Chlamydia-infected HeLa cell lysates	1103:1174	We designed a new method of muropeptide isolation consisting of a double filtration step coupled with reverse-phase HPLC fractionation of Chlamydia-infected HeLa cell lysates.
26290580	3	56	theme	protein	687:693	arg1	receptors					718:726	nucleotide-binding oligomerization domain-containing protein 2 (NOD2) innate immune receptors	634:726	nucleotide-binding oligomerization domain-containing protein 2 (NOD2) innate immune receptors	634:726	Pathogenic chlamydial species are known to activate nucleotide-binding oligomerization domain-containing protein 2 (NOD2) innate immune receptors by as yet uncharacterized ligands, which are presumed to be PG fragments (muramyl di- and tripeptides).
26290580	0	57	dep	Chlamydia	49:57	arg1	trachomatis					59:69	trachomatis	59:69	trachomatis	59:69	Structural characterization of muropeptides from Chlamydia trachomatis peptidoglycan by mass spectrometry resolves "chlamydial anomaly".
26290580	8	58	theme	"	1645:1645	arg1	hypothesis					1647:1656	the "glycanless peptidoglycan" hypothesis	1616:1656	the "glycanless peptidoglycan" hypothesis	1616:1656	These results reveal the composition of chlamydial PG and disprove the "glycanless peptidoglycan" hypothesis.
26290580	5	59	theme	lysates	1168:1174	arg1	fractionation					1122:1134	reverse-phase HPLC fractionation	1103:1134	reverse-phase HPLC fractionation of Chlamydia-infected HeLa cell lysates	1103:1174	We designed a new method of muropeptide isolation consisting of a double filtration step coupled with reverse-phase HPLC fractionation of Chlamydia-infected HeLa cell lysates.
26290580	2	60	theme	new	457:459	arg1	method					490:495	a new metabolic cell wall labeling method	455:495	a new metabolic cell wall labeling method	455:495	We recently detected PG in Chlamydia trachomatis by a new metabolic cell wall labeling method, however efforts to purify PG from pathogenic Chlamydiae have remained unsuccessful.
26290580	1	61	theme	genetic	307:313	arg1	evidence					331:338	genetic and biochemical evidence	307:338	genetic and biochemical evidence	307:338	The "chlamydial anomaly," first coined by James Moulder, describes the inability of researchers to detect or purify peptidoglycan (PG) from pathogenic Chlamydiae despite genetic and biochemical evidence and antibiotic susceptibility data that suggest its existence.
26290580	5	62	theme	HeLa	1158:1161	arg1	lysates					1168:1174	Chlamydia-infected HeLa cell lysates	1139:1174	Chlamydia-infected HeLa cell lysates	1139:1174	We designed a new method of muropeptide isolation consisting of a double filtration step coupled with reverse-phase HPLC fractionation of Chlamydia-infected HeLa cell lysates.
26290580	3	63	theme	innate	704:709	arg1	receptors					718:726	nucleotide-binding oligomerization domain-containing protein 2 (NOD2) innate immune receptors	634:726	nucleotide-binding oligomerization domain-containing protein 2 (NOD2) innate immune receptors	634:726	Pathogenic chlamydial species are known to activate nucleotide-binding oligomerization domain-containing protein 2 (NOD2) innate immune receptors by as yet uncharacterized ligands, which are presumed to be PG fragments (muramyl di- and tripeptides).
26290580	4	64	theme	NOD2-dependent	844:857	arg1	biomarker					924:932	a biomarker	922:932	a biomarker for the presence of PG fragments within specific lysate fractions	922:998	We used the NOD2-dependent activation of NF-κB by C. trachomatis-infected cell lysates as a biomarker for the presence of PG fragments within specific lysate fractions.
26290580	4	64	theme	NOD2-dependent	844:857	arg1	activation					859:868	the NOD2-dependent activation	840:868	the NOD2-dependent activation of NF-κB by C. trachomatis-infected cell lysates	840:917	We used the NOD2-dependent activation of NF-κB by C. trachomatis-infected cell lysates as a biomarker for the presence of PG fragments within specific lysate fractions.
26290580	3	65	theme	immune	711:716	arg1	receptors					718:726	nucleotide-binding oligomerization domain-containing protein 2 (NOD2) innate immune receptors	634:726	nucleotide-binding oligomerization domain-containing protein 2 (NOD2) innate immune receptors	634:726	Pathogenic chlamydial species are known to activate nucleotide-binding oligomerization domain-containing protein 2 (NOD2) innate immune receptors by as yet uncharacterized ligands, which are presumed to be PG fragments (muramyl di- and tripeptides).
26290580	3	66	theme	nucleotide-binding	634:651	arg1	receptors					718:726	nucleotide-binding oligomerization domain-containing protein 2 (NOD2) innate immune receptors	634:726	nucleotide-binding oligomerization domain-containing protein 2 (NOD2) innate immune receptors	634:726	Pathogenic chlamydial species are known to activate nucleotide-binding oligomerization domain-containing protein 2 (NOD2) innate immune receptors by as yet uncharacterized ligands, which are presumed to be PG fragments (muramyl di- and tripeptides).
26290580	6	67	theme	muramyl	1303:1309	arg1	di-					1311:1313	muramyl di-	1303:1313	muramyl di-	1303:1313	Fractions that displayed NOD2 activity were analyzed by electrospray ionization mass spectrometry, confirming the presence of muramyl di- and tripeptides in Chlamydia-infected cell lysate fractions.
26290580	6	68	theme	lysate	1358:1363	arg1	fractions					1365:1373	Chlamydia-infected cell lysate fractions	1334:1373	Chlamydia-infected cell lysate fractions	1334:1373	Fractions that displayed NOD2 activity were analyzed by electrospray ionization mass spectrometry, confirming the presence of muramyl di- and tripeptides in Chlamydia-infected cell lysate fractions.
26290580	3	69	theme	NOD2	698:701	arg1	receptors					718:726	nucleotide-binding oligomerization domain-containing protein 2 (NOD2) innate immune receptors	634:726	nucleotide-binding oligomerization domain-containing protein 2 (NOD2) innate immune receptors	634:726	Pathogenic chlamydial species are known to activate nucleotide-binding oligomerization domain-containing protein 2 (NOD2) innate immune receptors by as yet uncharacterized ligands, which are presumed to be PG fragments (muramyl di- and tripeptides).
26290580	3	70	theme	oligomerization	653:667	arg1	receptors					718:726	nucleotide-binding oligomerization domain-containing protein 2 (NOD2) innate immune receptors	634:726	nucleotide-binding oligomerization domain-containing protein 2 (NOD2) innate immune receptors	634:726	Pathogenic chlamydial species are known to activate nucleotide-binding oligomerization domain-containing protein 2 (NOD2) innate immune receptors by as yet uncharacterized ligands, which are presumed to be PG fragments (muramyl di- and tripeptides).
26290580	8	71	theme	PG	1600:1601	arg1	composition					1574:1584	the composition	1570:1584	the composition of chlamydial PG	1570:1601	These results reveal the composition of chlamydial PG and disprove the "glycanless peptidoglycan" hypothesis.
26290580	1	72	theme	chlamydial	142:151	arg1	"					161:161	"	161:161	" first coined by James Moulder	161:191	The "chlamydial anomaly," first coined by James Moulder, describes the inability of researchers to detect or purify peptidoglycan (PG) from pathogenic Chlamydiae despite genetic and biochemical evidence and antibiotic susceptibility data that suggest its existence.
26290580	1	72	theme	chlamydial	142:151	arg1	anomaly					153:159	The "chlamydial anomaly	137:159	The "chlamydial anomaly	137:159	The "chlamydial anomaly," first coined by James Moulder, describes the inability of researchers to detect or purify peptidoglycan (PG) from pathogenic Chlamydiae despite genetic and biochemical evidence and antibiotic susceptibility data that suggest its existence.
26290580	6	73	from	presence	1291:1298	arg1	fractions					1365:1373	Chlamydia-infected cell lysate fractions	1334:1373	Chlamydia-infected cell lysate fractions	1334:1373	Fractions that displayed NOD2 activity were analyzed by electrospray ionization mass spectrometry, confirming the presence of muramyl di- and tripeptides in Chlamydia-infected cell lysate fractions.
26290580	1	74	theme	researchers	221:231	arg1	inability					208:216	the inability	204:216	the inability of researchers to detect or purify peptidoglycan (PG) from pathogenic Chlamydiae despite genetic and biochemical evidence and antibiotic susceptibility data that suggest its existence	204:400	The "chlamydial anomaly," first coined by James Moulder, describes the inability of researchers to detect or purify peptidoglycan (PG) from pathogenic Chlamydiae despite genetic and biochemical evidence and antibiotic susceptibility data that suggest its existence.
26290580	0	75	theme	mass	88:91	arg1	spectrometry					93:104	mass spectrometry	88:104	mass spectrometry	88:104	Structural characterization of muropeptides from Chlamydia trachomatis peptidoglycan by mass spectrometry resolves "chlamydial anomaly".
26290580	5	76	theme	filtration	1074:1083	arg1	step					1085:1088	a double filtration step	1065:1088	a double filtration step coupled with reverse-phase HPLC fractionation of Chlamydia-infected HeLa cell lysates	1065:1174	We designed a new method of muropeptide isolation consisting of a double filtration step coupled with reverse-phase HPLC fractionation of Chlamydia-infected HeLa cell lysates.
26290580	5	77	theme	new	1015:1017	arg1	method					1019:1024	a new method	1013:1024	a new method of muropeptide isolation consisting of a double filtration step coupled with reverse-phase HPLC fractionation of Chlamydia-infected HeLa cell lysates	1013:1174	We designed a new method of muropeptide isolation consisting of a double filtration step coupled with reverse-phase HPLC fractionation of Chlamydia-infected HeLa cell lysates.
26290580	8	78	theme	peptidoglycan	1632:1644	arg1	hypothesis					1647:1656	the "glycanless peptidoglycan" hypothesis	1616:1656	the "glycanless peptidoglycan" hypothesis	1616:1656	These results reveal the composition of chlamydial PG and disprove the "glycanless peptidoglycan" hypothesis.
26290580	4	79	theme	lysate	983:988	arg1	fractions					990:998	specific lysate fractions	974:998	specific lysate fractions	974:998	We used the NOD2-dependent activation of NF-κB by C. trachomatis-infected cell lysates as a biomarker for the presence of PG fragments within specific lysate fractions.
26290580	1	80	theme	biochemical	319:329	arg1	evidence					331:338	genetic and biochemical evidence	307:338	genetic and biochemical evidence	307:338	The "chlamydial anomaly," first coined by James Moulder, describes the inability of researchers to detect or purify peptidoglycan (PG) from pathogenic Chlamydiae despite genetic and biochemical evidence and antibiotic susceptibility data that suggest its existence.
26290580	6	81	theme	electrospray	1233:1244	arg1	spectrometry					1262:1273	electrospray ionization mass spectrometry	1233:1273	electrospray ionization mass spectrometry	1233:1273	Fractions that displayed NOD2 activity were analyzed by electrospray ionization mass spectrometry, confirming the presence of muramyl di- and tripeptides in Chlamydia-infected cell lysate fractions.
26290580	8	82	theme	chlamydial	1589:1598	arg1	PG					1600:1601	chlamydial PG	1589:1601	chlamydial PG	1589:1601	These results reveal the composition of chlamydial PG and disprove the "glycanless peptidoglycan" hypothesis.
26290580	6	83	attach	presence	1291:1298	arg2	tripeptides					1319:1329	tripeptides	1319:1329	tripeptides	1319:1329	Fractions that displayed NOD2 activity were analyzed by electrospray ionization mass spectrometry, confirming the presence of muramyl di- and tripeptides in Chlamydia-infected cell lysate fractions.
26290580	6	83	attach	presence	1291:1298	arg1	fractions					1365:1373	Chlamydia-infected cell lysate fractions	1334:1373	Chlamydia-infected cell lysate fractions	1334:1373	Fractions that displayed NOD2 activity were analyzed by electrospray ionization mass spectrometry, confirming the presence of muramyl di- and tripeptides in Chlamydia-infected cell lysate fractions.
26290580	6	83	attach	presence	1291:1298	arg2	di-					1311:1313	muramyl di-	1303:1313	muramyl di-	1303:1313	Fractions that displayed NOD2 activity were analyzed by electrospray ionization mass spectrometry, confirming the presence of muramyl di- and tripeptides in Chlamydia-infected cell lysate fractions.
26476244	1	0	theme	size-exclusion	320:333	arg1	chromatography					335:348	anion-exchange and size-exclusion chromatography	301:348	chromatography	335:348	A novel water-soluble polysaccharide PUP60S2, with a molecular weight of 1.44×10(4)Da, was obtained from the sclerotia of Polyporus umbellatus by hot water extraction, ethanol precipitation, anion-exchange and size-exclusion chromatography.
26476244	1	0	theme	size-exclusion	320:333	arg1	extraction					266:275	hot water extraction	256:275	hot water extraction	256:275	A novel water-soluble polysaccharide PUP60S2, with a molecular weight of 1.44×10(4)Da, was obtained from the sclerotia of Polyporus umbellatus by hot water extraction, ethanol precipitation, anion-exchange and size-exclusion chromatography.
26476244	1	1	theme	molecular	163:171	arg1	weight					173:178	a molecular weight	161:178	a molecular weight of 1.44×10(4)Da	161:194	A novel water-soluble polysaccharide PUP60S2, with a molecular weight of 1.44×10(4)Da, was obtained from the sclerotia of Polyporus umbellatus by hot water extraction, ethanol precipitation, anion-exchange and size-exclusion chromatography.
26476244	5	2	from	effect	984:989	arg1	DPPH					994:997	DPPH	994:997	DPPH	994:997	The antioxidant activity assay in vitro showed that PUP60S2 exerted a significant scavenging effect on DPPH, hydroxyl radical and superoxide radical in a dose-dependent manner.
26476244	5	2	from	effect	984:989	arg1	radical					1009:1015	hydroxyl radical	1000:1015	hydroxyl radical	1000:1015	The antioxidant activity assay in vitro showed that PUP60S2 exerted a significant scavenging effect on DPPH, hydroxyl radical and superoxide radical in a dose-dependent manner.
26476244	5	2	from	effect	984:989	arg1	radical					1032:1038	superoxide radical	1021:1038	superoxide radical	1021:1038	The antioxidant activity assay in vitro showed that PUP60S2 exerted a significant scavenging effect on DPPH, hydroxyl radical and superoxide radical in a dose-dependent manner.
26476244	0	3	from	elucidation	10:20	arg1	Polyporus					78:86	Polyporus	78:86	Polyporus	78:86	Structure elucidation and antioxidant activity of a novel polysaccharide from Polyporus umbellatus sclerotia.
26476244	4	4	theme	-β-d-GlcpA	873:882	arg1	units					884:888	(1→4)-β-d-GlcpA units	868:888	(1→4)-β-d-GlcpA units	868:888	Reduction of carboxyl groups, sugar analysis, methylation analysis, together with one and two dimension NMR spectra disclosed that the backbone of PUP60S2 consisted of (1→6)-β-d-glucopyranosyl, every second of which was substituted at O-3 by side chains consisting of terminal β-d-Glcp, (1→3)-β-d-Glcp, (1→3)-β-d-GlcpA, (1→4)-β-d-Glcp and (1→4)-β-d-GlcpA units.
26476244	1	5	theme	umbellatus	242:251	arg1	sclerotia					219:227	the sclerotia	215:227	the sclerotia of Polyporus umbellatus	215:251	A novel water-soluble polysaccharide PUP60S2, with a molecular weight of 1.44×10(4)Da, was obtained from the sclerotia of Polyporus umbellatus by hot water extraction, ethanol precipitation, anion-exchange and size-exclusion chromatography.
26476244	5	6	theme	superoxide	1021:1030	arg1	DPPH					994:997	DPPH	994:997	DPPH	994:997	The antioxidant activity assay in vitro showed that PUP60S2 exerted a significant scavenging effect on DPPH, hydroxyl radical and superoxide radical in a dose-dependent manner.
26476244	5	6	theme	superoxide	1021:1030	arg1	radical					1032:1038	superoxide radical	1021:1038	superoxide radical	1021:1038	The antioxidant activity assay in vitro showed that PUP60S2 exerted a significant scavenging effect on DPPH, hydroxyl radical and superoxide radical in a dose-dependent manner.
26476244	5	7	theme	antioxidant	895:905	arg1	assay					916:920	The antioxidant activity assay	891:920	The antioxidant activity assay in vitro	891:929	The antioxidant activity assay in vitro showed that PUP60S2 exerted a significant scavenging effect on DPPH, hydroxyl radical and superoxide radical in a dose-dependent manner.
26476244	3	8	theme	glucuronic	512:521	arg1	acid					523:526	glucuronic acid	512:526	glucuronic acid	512:526	Monosaccharide composition analysis showed that PUP60S2 was only comprised of glucose and glucuronic acid.
26476244	4	9	theme	-β-d-glucopyranosyl	702:720	arg1	second					729:734	second	729:734	second	729:734	Reduction of carboxyl groups, sugar analysis, methylation analysis, together with one and two dimension NMR spectra disclosed that the backbone of PUP60S2 consisted of (1→6)-β-d-glucopyranosyl, every second of which was substituted at O-3 by side chains consisting of terminal β-d-Glcp, (1→3)-β-d-Glcp, (1→3)-β-d-GlcpA, (1→4)-β-d-Glcp and (1→4)-β-d-GlcpA units.
26476244	1	10	with	PUP60S2	147:153	arg1	weight					173:178	a molecular weight	161:178	a molecular weight of 1.44×10(4)Da	161:194	A novel water-soluble polysaccharide PUP60S2, with a molecular weight of 1.44×10(4)Da, was obtained from the sclerotia of Polyporus umbellatus by hot water extraction, ethanol precipitation, anion-exchange and size-exclusion chromatography.
26476244	4	11	theme	NMR	633:635	arg1	groups					551:556	carboxyl groups	542:556	carboxyl groups	542:556	Reduction of carboxyl groups, sugar analysis, methylation analysis, together with one and two dimension NMR spectra disclosed that the backbone of PUP60S2 consisted of (1→6)-β-d-glucopyranosyl, every second of which was substituted at O-3 by side chains consisting of terminal β-d-Glcp, (1→3)-β-d-Glcp, (1→3)-β-d-GlcpA, (1→4)-β-d-Glcp and (1→4)-β-d-GlcpA units.
26476244	4	11	theme	NMR	633:635	arg1	spectra					637:643	one and two dimension NMR spectra	611:643	one and two dimension NMR spectra	611:643	Reduction of carboxyl groups, sugar analysis, methylation analysis, together with one and two dimension NMR spectra disclosed that the backbone of PUP60S2 consisted of (1→6)-β-d-glucopyranosyl, every second of which was substituted at O-3 by side chains consisting of terminal β-d-Glcp, (1→3)-β-d-Glcp, (1→3)-β-d-GlcpA, (1→4)-β-d-Glcp and (1→4)-β-d-GlcpA units.
26476244	5	12	theme	activity	907:914	arg1	assay					916:920	The antioxidant activity assay	891:920	The antioxidant activity assay in vitro	891:929	The antioxidant activity assay in vitro showed that PUP60S2 exerted a significant scavenging effect on DPPH, hydroxyl radical and superoxide radical in a dose-dependent manner.
26476244	4	13	theme	PUP60S2	676:682	arg1	backbone					664:671	the backbone	660:671	the backbone of PUP60S2	660:682	Reduction of carboxyl groups, sugar analysis, methylation analysis, together with one and two dimension NMR spectra disclosed that the backbone of PUP60S2 consisted of (1→6)-β-d-glucopyranosyl, every second of which was substituted at O-3 by side chains consisting of terminal β-d-Glcp, (1→3)-β-d-Glcp, (1→3)-β-d-GlcpA, (1→4)-β-d-Glcp and (1→4)-β-d-GlcpA units.
26476244	4	14	dep	-β-d-Glcp	854:862	arg1	1→4					850:852	1→4	850:852	1→4	850:852	Reduction of carboxyl groups, sugar analysis, methylation analysis, together with one and two dimension NMR spectra disclosed that the backbone of PUP60S2 consisted of (1→6)-β-d-glucopyranosyl, every second of which was substituted at O-3 by side chains consisting of terminal β-d-Glcp, (1→3)-β-d-Glcp, (1→3)-β-d-GlcpA, (1→4)-β-d-Glcp and (1→4)-β-d-GlcpA units.
26476244	4	15	dep	-β-d-Glcp	821:829	arg1	1→3					817:819	1→3	817:819	1→3	817:819	Reduction of carboxyl groups, sugar analysis, methylation analysis, together with one and two dimension NMR spectra disclosed that the backbone of PUP60S2 consisted of (1→6)-β-d-glucopyranosyl, every second of which was substituted at O-3 by side chains consisting of terminal β-d-Glcp, (1→3)-β-d-Glcp, (1→3)-β-d-GlcpA, (1→4)-β-d-Glcp and (1→4)-β-d-GlcpA units.
26476244	0	16	from	activity	38:45	arg1	Polyporus					78:86	Polyporus	78:86	Polyporus	78:86	Structure elucidation and antioxidant activity of a novel polysaccharide from Polyporus umbellatus sclerotia.
26476244	4	17	dep	together	597:604	arg1	with					606:609	with	606:609	with	606:609	Reduction of carboxyl groups, sugar analysis, methylation analysis, together with one and two dimension NMR spectra disclosed that the backbone of PUP60S2 consisted of (1→6)-β-d-glucopyranosyl, every second of which was substituted at O-3 by side chains consisting of terminal β-d-Glcp, (1→3)-β-d-Glcp, (1→3)-β-d-GlcpA, (1→4)-β-d-Glcp and (1→4)-β-d-GlcpA units.
26476244	3	18	theme	Monosaccharide	422:435	arg1	analysis					449:456	Monosaccharide composition analysis	422:456	Monosaccharide composition analysis	422:456	Monosaccharide composition analysis showed that PUP60S2 was only comprised of glucose and glucuronic acid.
26476244	0	19	theme	Structure	0:8	arg1	elucidation					10:20	Structure elucidation	0:20	Structure elucidation	0:20	Structure elucidation and antioxidant activity of a novel polysaccharide from Polyporus umbellatus sclerotia.
26476244	1	20	theme	hot	256:258	arg1	extraction					266:275	hot water extraction	256:275	hot water extraction	256:275	A novel water-soluble polysaccharide PUP60S2, with a molecular weight of 1.44×10(4)Da, was obtained from the sclerotia of Polyporus umbellatus by hot water extraction, ethanol precipitation, anion-exchange and size-exclusion chromatography.
26476244	1	20	theme	hot	256:258	arg1	precipitation					286:298	ethanol precipitation	278:298	ethanol precipitation	278:298	A novel water-soluble polysaccharide PUP60S2, with a molecular weight of 1.44×10(4)Da, was obtained from the sclerotia of Polyporus umbellatus by hot water extraction, ethanol precipitation, anion-exchange and size-exclusion chromatography.
26476244	1	20	theme	hot	256:258	arg1	chromatography					335:348	anion-exchange and size-exclusion chromatography	301:348	chromatography	335:348	A novel water-soluble polysaccharide PUP60S2, with a molecular weight of 1.44×10(4)Da, was obtained from the sclerotia of Polyporus umbellatus by hot water extraction, ethanol precipitation, anion-exchange and size-exclusion chromatography.
26476244	0	21	dep	Polyporus	78:86	arg1	sclerotia					99:107	Polyporus umbellatus sclerotia	78:107	Polyporus umbellatus sclerotia	78:107	Structure elucidation and antioxidant activity of a novel polysaccharide from Polyporus umbellatus sclerotia.
26476244	3	22	theme	composition	437:447	arg1	analysis					449:456	Monosaccharide composition analysis	422:456	Monosaccharide composition analysis	422:456	Monosaccharide composition analysis showed that PUP60S2 was only comprised of glucose and glucuronic acid.
26476244	0	23	theme	antioxidant	26:36	arg1	activity					38:45	antioxidant activity	26:45	antioxidant activity	26:45	Structure elucidation and antioxidant activity of a novel polysaccharide from Polyporus umbellatus sclerotia.
26476244	1	24	theme	water	260:264	arg1	extraction					266:275	hot water extraction	256:275	hot water extraction	256:275	A novel water-soluble polysaccharide PUP60S2, with a molecular weight of 1.44×10(4)Da, was obtained from the sclerotia of Polyporus umbellatus by hot water extraction, ethanol precipitation, anion-exchange and size-exclusion chromatography.
26476244	1	24	theme	water	260:264	arg1	precipitation					286:298	ethanol precipitation	278:298	ethanol precipitation	278:298	A novel water-soluble polysaccharide PUP60S2, with a molecular weight of 1.44×10(4)Da, was obtained from the sclerotia of Polyporus umbellatus by hot water extraction, ethanol precipitation, anion-exchange and size-exclusion chromatography.
26476244	1	24	theme	water	260:264	arg1	chromatography					335:348	anion-exchange and size-exclusion chromatography	301:348	chromatography	335:348	A novel water-soluble polysaccharide PUP60S2, with a molecular weight of 1.44×10(4)Da, was obtained from the sclerotia of Polyporus umbellatus by hot water extraction, ethanol precipitation, anion-exchange and size-exclusion chromatography.
26476244	5	25	theme	significant	961:971	arg1	effect					984:989	a significant scavenging effect	959:989	a significant scavenging effect on DPPH, hydroxyl radical and superoxide radical	959:1038	The antioxidant activity assay in vitro showed that PUP60S2 exerted a significant scavenging effect on DPPH, hydroxyl radical and superoxide radical in a dose-dependent manner.
26476244	4	26	dep	-β-d-glucopyranosyl	702:720	arg1	1→6					698:700	1→6	698:700	1→6	698:700	Reduction of carboxyl groups, sugar analysis, methylation analysis, together with one and two dimension NMR spectra disclosed that the backbone of PUP60S2 consisted of (1→6)-β-d-glucopyranosyl, every second of which was substituted at O-3 by side chains consisting of terminal β-d-Glcp, (1→3)-β-d-Glcp, (1→3)-β-d-GlcpA, (1→4)-β-d-Glcp and (1→4)-β-d-GlcpA units.
26476244	4	27	theme	methylation	575:585	arg1	groups					551:556	carboxyl groups	542:556	carboxyl groups	542:556	Reduction of carboxyl groups, sugar analysis, methylation analysis, together with one and two dimension NMR spectra disclosed that the backbone of PUP60S2 consisted of (1→6)-β-d-glucopyranosyl, every second of which was substituted at O-3 by side chains consisting of terminal β-d-Glcp, (1→3)-β-d-Glcp, (1→3)-β-d-GlcpA, (1→4)-β-d-Glcp and (1→4)-β-d-GlcpA units.
26476244	4	27	theme	methylation	575:585	arg1	analysis					587:594	methylation analysis	575:594	methylation analysis	575:594	Reduction of carboxyl groups, sugar analysis, methylation analysis, together with one and two dimension NMR spectra disclosed that the backbone of PUP60S2 consisted of (1→6)-β-d-glucopyranosyl, every second of which was substituted at O-3 by side chains consisting of terminal β-d-Glcp, (1→3)-β-d-Glcp, (1→3)-β-d-GlcpA, (1→4)-β-d-Glcp and (1→4)-β-d-GlcpA units.
26476244	1	28	theme	novel	112:116	arg1	PUP60S2					147:153	A novel water-soluble polysaccharide PUP60S2	110:153	A novel water-soluble polysaccharide PUP60S2	110:153	A novel water-soluble polysaccharide PUP60S2, with a molecular weight of 1.44×10(4)Da, was obtained from the sclerotia of Polyporus umbellatus by hot water extraction, ethanol precipitation, anion-exchange and size-exclusion chromatography.
26476244	1	29	theme	Da	193:194	arg1	weight					173:178	a molecular weight	161:178	a molecular weight of 1.44×10(4)Da	161:194	A novel water-soluble polysaccharide PUP60S2, with a molecular weight of 1.44×10(4)Da, was obtained from the sclerotia of Polyporus umbellatus by hot water extraction, ethanol precipitation, anion-exchange and size-exclusion chromatography.
26476244	4	30	dep	-β-d-GlcpA	837:846	arg1	1→3					833:835	1→3	833:835	1→3	833:835	Reduction of carboxyl groups, sugar analysis, methylation analysis, together with one and two dimension NMR spectra disclosed that the backbone of PUP60S2 consisted of (1→6)-β-d-glucopyranosyl, every second of which was substituted at O-3 by side chains consisting of terminal β-d-Glcp, (1→3)-β-d-Glcp, (1→3)-β-d-GlcpA, (1→4)-β-d-Glcp and (1→4)-β-d-GlcpA units.
26476244	4	31	theme	1→4	869:871	arg1	units					884:888	(1→4)-β-d-GlcpA units	868:888	(1→4)-β-d-GlcpA units	868:888	Reduction of carboxyl groups, sugar analysis, methylation analysis, together with one and two dimension NMR spectra disclosed that the backbone of PUP60S2 consisted of (1→6)-β-d-glucopyranosyl, every second of which was substituted at O-3 by side chains consisting of terminal β-d-Glcp, (1→3)-β-d-Glcp, (1→3)-β-d-GlcpA, (1→4)-β-d-Glcp and (1→4)-β-d-GlcpA units.
26476244	0	32	from	Polyporus	78:86	arg1	polysaccharide					58:71	a novel polysaccharide	50:71	a novel polysaccharide from Polyporus umbellatus sclerotia	50:107	Structure elucidation and antioxidant activity of a novel polysaccharide from Polyporus umbellatus sclerotia.
26476244	0	32	from	Polyporus	78:86	arg1	elucidation					10:20	Structure elucidation	0:20	Structure elucidation	0:20	Structure elucidation and antioxidant activity of a novel polysaccharide from Polyporus umbellatus sclerotia.
26476244	0	32	from	Polyporus	78:86	arg1	activity					38:45	antioxidant activity	26:45	antioxidant activity	26:45	Structure elucidation and antioxidant activity of a novel polysaccharide from Polyporus umbellatus sclerotia.
26476244	2	33	theme	glucuronic	405:414	arg1	acid					416:419	about 22.3% glucuronic acid	393:419	about 22.3% glucuronic acid	393:419	PUP60S2 was a polysaccharide comprised of about 22.3% glucuronic acid.
26476244	1	34	theme	ethanol	278:284	arg1	extraction					266:275	hot water extraction	256:275	hot water extraction	256:275	A novel water-soluble polysaccharide PUP60S2, with a molecular weight of 1.44×10(4)Da, was obtained from the sclerotia of Polyporus umbellatus by hot water extraction, ethanol precipitation, anion-exchange and size-exclusion chromatography.
26476244	1	34	theme	ethanol	278:284	arg1	precipitation					286:298	ethanol precipitation	278:298	ethanol precipitation	278:298	A novel water-soluble polysaccharide PUP60S2, with a molecular weight of 1.44×10(4)Da, was obtained from the sclerotia of Polyporus umbellatus by hot water extraction, ethanol precipitation, anion-exchange and size-exclusion chromatography.
26476244	2	35	dep	%	403:403	arg1	22.3					399:402	22.3	399:402	22.3	399:402	PUP60S2 was a polysaccharide comprised of about 22.3% glucuronic acid.
26476244	4	36	theme	groups	551:556	arg1	Reduction					529:537	Reduction	529:537	Reduction of carboxyl groups, sugar analysis, methylation analysis, together with one and two dimension NMR spectra	529:643	Reduction of carboxyl groups, sugar analysis, methylation analysis, together with one and two dimension NMR spectra disclosed that the backbone of PUP60S2 consisted of (1→6)-β-d-glucopyranosyl, every second of which was substituted at O-3 by side chains consisting of terminal β-d-Glcp, (1→3)-β-d-Glcp, (1→3)-β-d-GlcpA, (1→4)-β-d-Glcp and (1→4)-β-d-GlcpA units.
26476244	0	37	theme	novel	52:56	arg1	polysaccharide					58:71	a novel polysaccharide	50:71	a novel polysaccharide from Polyporus umbellatus sclerotia	50:107	Structure elucidation and antioxidant activity of a novel polysaccharide from Polyporus umbellatus sclerotia.
26476244	4	38	theme	carboxyl	542:549	arg1	groups					551:556	carboxyl groups	542:556	carboxyl groups	542:556	Reduction of carboxyl groups, sugar analysis, methylation analysis, together with one and two dimension NMR spectra disclosed that the backbone of PUP60S2 consisted of (1→6)-β-d-glucopyranosyl, every second of which was substituted at O-3 by side chains consisting of terminal β-d-Glcp, (1→3)-β-d-Glcp, (1→3)-β-d-GlcpA, (1→4)-β-d-Glcp and (1→4)-β-d-GlcpA units.
26476244	4	38	theme	carboxyl	542:549	arg1	analysis					565:572	sugar analysis	559:572	sugar analysis	559:572	Reduction of carboxyl groups, sugar analysis, methylation analysis, together with one and two dimension NMR spectra disclosed that the backbone of PUP60S2 consisted of (1→6)-β-d-glucopyranosyl, every second of which was substituted at O-3 by side chains consisting of terminal β-d-Glcp, (1→3)-β-d-Glcp, (1→3)-β-d-GlcpA, (1→4)-β-d-Glcp and (1→4)-β-d-GlcpA units.
26476244	4	38	theme	carboxyl	542:549	arg1	analysis					587:594	methylation analysis	575:594	methylation analysis	575:594	Reduction of carboxyl groups, sugar analysis, methylation analysis, together with one and two dimension NMR spectra disclosed that the backbone of PUP60S2 consisted of (1→6)-β-d-glucopyranosyl, every second of which was substituted at O-3 by side chains consisting of terminal β-d-Glcp, (1→3)-β-d-Glcp, (1→3)-β-d-GlcpA, (1→4)-β-d-Glcp and (1→4)-β-d-GlcpA units.
26476244	4	38	theme	carboxyl	542:549	arg1	spectra					637:643	one and two dimension NMR spectra	611:643	one and two dimension NMR spectra	611:643	Reduction of carboxyl groups, sugar analysis, methylation analysis, together with one and two dimension NMR spectra disclosed that the backbone of PUP60S2 consisted of (1→6)-β-d-glucopyranosyl, every second of which was substituted at O-3 by side chains consisting of terminal β-d-Glcp, (1→3)-β-d-Glcp, (1→3)-β-d-GlcpA, (1→4)-β-d-Glcp and (1→4)-β-d-GlcpA units.
26476244	4	39	theme	sugar	559:563	arg1	groups					551:556	carboxyl groups	542:556	carboxyl groups	542:556	Reduction of carboxyl groups, sugar analysis, methylation analysis, together with one and two dimension NMR spectra disclosed that the backbone of PUP60S2 consisted of (1→6)-β-d-glucopyranosyl, every second of which was substituted at O-3 by side chains consisting of terminal β-d-Glcp, (1→3)-β-d-Glcp, (1→3)-β-d-GlcpA, (1→4)-β-d-Glcp and (1→4)-β-d-GlcpA units.
26476244	4	39	theme	sugar	559:563	arg1	analysis					565:572	sugar analysis	559:572	sugar analysis	559:572	Reduction of carboxyl groups, sugar analysis, methylation analysis, together with one and two dimension NMR spectra disclosed that the backbone of PUP60S2 consisted of (1→6)-β-d-glucopyranosyl, every second of which was substituted at O-3 by side chains consisting of terminal β-d-Glcp, (1→3)-β-d-Glcp, (1→3)-β-d-GlcpA, (1→4)-β-d-Glcp and (1→4)-β-d-GlcpA units.
26476244	1	40	theme	water-soluble	118:130	arg1	PUP60S2					147:153	A novel water-soluble polysaccharide PUP60S2	110:153	A novel water-soluble polysaccharide PUP60S2	110:153	A novel water-soluble polysaccharide PUP60S2, with a molecular weight of 1.44×10(4)Da, was obtained from the sclerotia of Polyporus umbellatus by hot water extraction, ethanol precipitation, anion-exchange and size-exclusion chromatography.
26476244	5	41	theme	scavenging	973:982	arg1	effect					984:989	a significant scavenging effect	959:989	a significant scavenging effect on DPPH, hydroxyl radical and superoxide radical	959:1038	The antioxidant activity assay in vitro showed that PUP60S2 exerted a significant scavenging effect on DPPH, hydroxyl radical and superoxide radical in a dose-dependent manner.
26476244	2	42	dep	acid	416:419	arg1	%					403:403	%	403:403	%	403:403	PUP60S2 was a polysaccharide comprised of about 22.3% glucuronic acid.
26476244	1	43	theme	polysaccharide	132:145	arg1	PUP60S2					147:153	A novel water-soluble polysaccharide PUP60S2	110:153	A novel water-soluble polysaccharide PUP60S2	110:153	A novel water-soluble polysaccharide PUP60S2, with a molecular weight of 1.44×10(4)Da, was obtained from the sclerotia of Polyporus umbellatus by hot water extraction, ethanol precipitation, anion-exchange and size-exclusion chromatography.
26476244	1	44	theme	anion-exchange	301:314	arg1	chromatography					335:348	anion-exchange and size-exclusion chromatography	301:348	chromatography	335:348	A novel water-soluble polysaccharide PUP60S2, with a molecular weight of 1.44×10(4)Da, was obtained from the sclerotia of Polyporus umbellatus by hot water extraction, ethanol precipitation, anion-exchange and size-exclusion chromatography.
26476244	1	44	theme	anion-exchange	301:314	arg1	extraction					266:275	hot water extraction	256:275	hot water extraction	256:275	A novel water-soluble polysaccharide PUP60S2, with a molecular weight of 1.44×10(4)Da, was obtained from the sclerotia of Polyporus umbellatus by hot water extraction, ethanol precipitation, anion-exchange and size-exclusion chromatography.
26476244	4	45	theme	terminal	797:804	arg1	β-d-Glcp					806:813	terminal β-d-Glcp	797:813	terminal β-d-Glcp	797:813	Reduction of carboxyl groups, sugar analysis, methylation analysis, together with one and two dimension NMR spectra disclosed that the backbone of PUP60S2 consisted of (1→6)-β-d-glucopyranosyl, every second of which was substituted at O-3 by side chains consisting of terminal β-d-Glcp, (1→3)-β-d-Glcp, (1→3)-β-d-GlcpA, (1→4)-β-d-Glcp and (1→4)-β-d-GlcpA units.
26476244	4	46	theme	side	771:774	arg1	chains					776:781	side chains	771:781	side chains consisting of terminal β-d-Glcp, (1→3)-β-d-Glcp, (1→3)-β-d-GlcpA, (1→4)-β-d-Glcp and (1→4)-β-d-GlcpA units	771:888	Reduction of carboxyl groups, sugar analysis, methylation analysis, together with one and two dimension NMR spectra disclosed that the backbone of PUP60S2 consisted of (1→6)-β-d-glucopyranosyl, every second of which was substituted at O-3 by side chains consisting of terminal β-d-Glcp, (1→3)-β-d-Glcp, (1→3)-β-d-GlcpA, (1→4)-β-d-Glcp and (1→4)-β-d-GlcpA units.
26476244	5	47	theme	hydroxyl	1000:1007	arg1	DPPH					994:997	DPPH	994:997	DPPH	994:997	The antioxidant activity assay in vitro showed that PUP60S2 exerted a significant scavenging effect on DPPH, hydroxyl radical and superoxide radical in a dose-dependent manner.
26476244	5	47	theme	hydroxyl	1000:1007	arg1	radical					1009:1015	hydroxyl radical	1000:1015	hydroxyl radical	1000:1015	The antioxidant activity assay in vitro showed that PUP60S2 exerted a significant scavenging effect on DPPH, hydroxyl radical and superoxide radical in a dose-dependent manner.
26476244	4	48	theme	dimension	623:631	arg1	groups					551:556	carboxyl groups	542:556	carboxyl groups	542:556	Reduction of carboxyl groups, sugar analysis, methylation analysis, together with one and two dimension NMR spectra disclosed that the backbone of PUP60S2 consisted of (1→6)-β-d-glucopyranosyl, every second of which was substituted at O-3 by side chains consisting of terminal β-d-Glcp, (1→3)-β-d-Glcp, (1→3)-β-d-GlcpA, (1→4)-β-d-Glcp and (1→4)-β-d-GlcpA units.
26476244	4	48	theme	dimension	623:631	arg1	spectra					637:643	one and two dimension NMR spectra	611:643	one and two dimension NMR spectra	611:643	Reduction of carboxyl groups, sugar analysis, methylation analysis, together with one and two dimension NMR spectra disclosed that the backbone of PUP60S2 consisted of (1→6)-β-d-glucopyranosyl, every second of which was substituted at O-3 by side chains consisting of terminal β-d-Glcp, (1→3)-β-d-Glcp, (1→3)-β-d-GlcpA, (1→4)-β-d-Glcp and (1→4)-β-d-GlcpA units.
26476244	0	49	theme	polysaccharide	58:71	arg1	elucidation					10:20	Structure elucidation	0:20	Structure elucidation	0:20	Structure elucidation and antioxidant activity of a novel polysaccharide from Polyporus umbellatus sclerotia.
26476244	0	49	theme	polysaccharide	58:71	arg1	activity					38:45	antioxidant activity	26:45	antioxidant activity	26:45	Structure elucidation and antioxidant activity of a novel polysaccharide from Polyporus umbellatus sclerotia.
26476244	5	50	theme	dose-dependent	1045:1058	arg1	manner					1060:1065	a dose-dependent manner	1043:1065	a dose-dependent manner	1043:1065	The antioxidant activity assay in vitro showed that PUP60S2 exerted a significant scavenging effect on DPPH, hydroxyl radical and superoxide radical in a dose-dependent manner.
26277184	9	0	theme	bioactive	1401:1409	arg1	properties					1429:1438	the bioactive and biocompatible properties	1397:1438	the bioactive and biocompatible properties of this composite	1397:1456	The results indicate the bioactive and biocompatible properties of this composite and demonstrate how Raman and FTIR spectroscopic imaging have been used as an effective tool for tissue characterisation.
26277184	7	1	theme	imaging	1177:1183	arg1	suitability					1145:1155	the suitability	1141:1155	the suitability of FTIR microscopic imaging with lenses in studying these samples	1141:1221	The presence of collagen and lipids at the bone/composite interface confirmed biocompatibility and demonstrate the suitability of FTIR microscopic imaging with lenses in studying these samples.
26277184	1	2	theme	FTIR	176:179	arg1	imaging					181:187	FTIR imaging	176:187	FTIR imaging	176:187	The spectroscopic approaches of FTIR imaging and Raman mapping were applied to the characterisation of a new carbon hydroxyapatite/β-glucan composite developed for bone tissue engineering.
26277184	1	3	theme	hydroxyapatite/β-glucan	260:282	arg1	composite					284:292	a new carbon hydroxyapatite/β-glucan composite	247:292	a new carbon hydroxyapatite/β-glucan composite developed for bone tissue engineering	247:330	The spectroscopic approaches of FTIR imaging and Raman mapping were applied to the characterisation of a new carbon hydroxyapatite/β-glucan composite developed for bone tissue engineering.
26277184	4	4	theme	spectroscopic	546:558	arg1	methods					576:582	spectroscopic and chemometric methods	546:582	spectroscopic and chemometric methods	546:582	Using spectroscopic and chemometric methods, we were able to determine the presence of amides and phosphates and the distribution of lipid-rich domains in the bone tissue, providing an assessment of the composite's bioactivity.
26277184	6	5	used	used	904:907	arg2	lenses					887:892	CaF2 lenses	882:892	CaF2 lenses	882:892	CaF2 lenses were also used on the infrared microscope to improve spectral quality by reducing scattering artefacts, improving chemometric analysis.
26277184	8	6	theme	good	1266:1269	arg1	composite					1246:1254	the composite	1242:1254	the composite	1242:1254	It confirmed that the composite is a very good background for collagen growth and increases collagen maturity with the time of the bone growth process.
26277184	8	6	theme	good	1266:1269	arg1	background					1271:1280	a very good background	1259:1280	a very good background for collagen growth	1259:1300	It confirmed that the composite is a very good background for collagen growth and increases collagen maturity with the time of the bone growth process.
26277184	1	7	theme	imaging	181:187	arg1	approaches					162:171	The spectroscopic approaches	144:171	The spectroscopic approaches of FTIR imaging and Raman mapping	144:205	The spectroscopic approaches of FTIR imaging and Raman mapping were applied to the characterisation of a new carbon hydroxyapatite/β-glucan composite developed for bone tissue engineering.
26277184	6	8	theme	CaF2	882:885	arg1	lenses					887:892	CaF2 lenses	882:892	CaF2 lenses	882:892	CaF2 lenses were also used on the infrared microscope to improve spectral quality by reducing scattering artefacts, improving chemometric analysis.
26277184	1	9	theme	composite	284:292	arg1	characterisation					227:242	the characterisation	223:242	the characterisation of a new carbon hydroxyapatite/β-glucan composite developed for bone tissue engineering	223:330	The spectroscopic approaches of FTIR imaging and Raman mapping were applied to the characterisation of a new carbon hydroxyapatite/β-glucan composite developed for bone tissue engineering.
26277184	6	10	theme	infrared	916:923	arg1	microscope					925:934	the infrared microscope	912:934	the infrared microscope	912:934	CaF2 lenses were also used on the infrared microscope to improve spectral quality by reducing scattering artefacts, improving chemometric analysis.
26277184	7	11	theme	FTIR	1160:1163	arg1	imaging					1177:1183	FTIR microscopic imaging	1160:1183	FTIR microscopic imaging	1160:1183	The presence of collagen and lipids at the bone/composite interface confirmed biocompatibility and demonstrate the suitability of FTIR microscopic imaging with lenses in studying these samples.
26277184	4	12	from	tissue	704:709	arg1	presence					615:622	the presence	611:622	the presence of amides and phosphates	611:647	Using spectroscopic and chemometric methods, we were able to determine the presence of amides and phosphates and the distribution of lipid-rich domains in the bone tissue, providing an assessment of the composite's bioactivity.
26277184	4	12	from	tissue	704:709	arg1	distribution					657:668	the distribution	653:668	the distribution of lipid-rich domains in the bone tissue	653:709	Using spectroscopic and chemometric methods, we were able to determine the presence of amides and phosphates and the distribution of lipid-rich domains in the bone tissue, providing an assessment of the composite's bioactivity.
26277184	0	13	theme	Raman	106:110	arg1	imaging					135:141	Raman and FTIR spectroscopic imaging	106:141	imaging	135:141	The biocompatibility of carbon hydroxyapatite/β-glucan composite for bone tissue engineering studied with Raman and FTIR spectroscopic imaging.
26277184	9	14	theme	tissue	1555:1560	arg1	characterisation					1562:1577	tissue characterisation	1555:1577	tissue characterisation	1555:1577	The results indicate the bioactive and biocompatible properties of this composite and demonstrate how Raman and FTIR spectroscopic imaging have been used as an effective tool for tissue characterisation.
26277184	6	15	theme	chemometric	1008:1018	arg1	analysis					1020:1027	chemometric analysis	1008:1027	chemometric analysis	1008:1027	CaF2 lenses were also used on the infrared microscope to improve spectral quality by reducing scattering artefacts, improving chemometric analysis.
26277184	2	16	theme	apatite-forming	386:400	arg1	ability					402:408	an apatite-forming ability	383:408	an apatite-forming ability for the bone repair process	383:436	The composite is an artificial bone material with an apatite-forming ability for the bone repair process.
26277184	2	17	theme	artificial	353:362	arg1	material					369:376	an artificial bone material	350:376	an artificial bone material with an apatite-forming ability for the bone repair process	350:436	The composite is an artificial bone material with an apatite-forming ability for the bone repair process.
26277184	2	17	theme	artificial	353:362	arg1	composite					337:345	The composite	333:345	The composite	333:345	The composite is an artificial bone material with an apatite-forming ability for the bone repair process.
26277184	0	18	theme	spectroscopic	121:133	arg1	imaging					135:141	Raman and FTIR spectroscopic imaging	106:141	imaging	135:141	The biocompatibility of carbon hydroxyapatite/β-glucan composite for bone tissue engineering studied with Raman and FTIR spectroscopic imaging.
26277184	8	19	with	maturity	1325:1332	arg1	time					1343:1346	the time	1339:1346	the time of the bone growth process	1339:1373	It confirmed that the composite is a very good background for collagen growth and increases collagen maturity with the time of the bone growth process.
26277184	1	20	theme	mapping	199:205	arg1	approaches					162:171	The spectroscopic approaches	144:171	The spectroscopic approaches of FTIR imaging and Raman mapping	144:205	The spectroscopic approaches of FTIR imaging and Raman mapping were applied to the characterisation of a new carbon hydroxyapatite/β-glucan composite developed for bone tissue engineering.
26277184	8	21	theme	collagen	1286:1293	arg1	growth					1295:1300	collagen growth	1286:1300	collagen growth	1286:1300	It confirmed that the composite is a very good background for collagen growth and increases collagen maturity with the time of the bone growth process.
26277184	0	22	theme	FTIR	116:119	arg1	imaging					135:141	Raman and FTIR spectroscopic imaging	106:141	imaging	135:141	The biocompatibility of carbon hydroxyapatite/β-glucan composite for bone tissue engineering studied with Raman and FTIR spectroscopic imaging.
26277184	3	23	theme	bone	446:449	arg1	samples					451:457	Rabbit bone samples	439:457	Rabbit bone samples	439:457	Rabbit bone samples were tested with an implanted bioactive material for a period of several months.
26277184	9	24	used	used	1525:1528	arg2	Raman					1478:1482	Raman	1478:1482	Raman	1478:1482	The results indicate the bioactive and biocompatible properties of this composite and demonstrate how Raman and FTIR spectroscopic imaging have been used as an effective tool for tissue characterisation.
26277184	9	24	used	used	1525:1528	arg2	tool					1546:1549	an effective tool	1533:1549	an effective tool for tissue characterisation	1533:1577	The results indicate the bioactive and biocompatible properties of this composite and demonstrate how Raman and FTIR spectroscopic imaging have been used as an effective tool for tissue characterisation.
26277184	9	24	used	used	1525:1528	arg2	imaging					1507:1513	FTIR spectroscopic imaging	1488:1513	FTIR spectroscopic imaging	1488:1513	The results indicate the bioactive and biocompatible properties of this composite and demonstrate how Raman and FTIR spectroscopic imaging have been used as an effective tool for tissue characterisation.
26277184	1	25	theme	Raman	193:197	arg1	mapping					199:205	Raman mapping	193:205	Raman mapping	193:205	The spectroscopic approaches of FTIR imaging and Raman mapping were applied to the characterisation of a new carbon hydroxyapatite/β-glucan composite developed for bone tissue engineering.
26277184	6	26	theme	scattering	976:985	arg1	artefacts					987:995	scattering artefacts	976:995	scattering artefacts	976:995	CaF2 lenses were also used on the infrared microscope to improve spectral quality by reducing scattering artefacts, improving chemometric analysis.
26277184	4	27	theme	domains	684:690	arg1	presence					615:622	the presence	611:622	the presence of amides and phosphates	611:647	Using spectroscopic and chemometric methods, we were able to determine the presence of amides and phosphates and the distribution of lipid-rich domains in the bone tissue, providing an assessment of the composite's bioactivity.
26277184	4	27	theme	domains	684:690	arg1	distribution					657:668	the distribution	653:668	the distribution of lipid-rich domains in the bone tissue	653:709	Using spectroscopic and chemometric methods, we were able to determine the presence of amides and phosphates and the distribution of lipid-rich domains in the bone tissue, providing an assessment of the composite's bioactivity.
26277184	4	28	theme	bioactivity	755:765	arg1	assessment					725:734	an assessment	722:734	an assessment of the composite's bioactivity	722:765	Using spectroscopic and chemometric methods, we were able to determine the presence of amides and phosphates and the distribution of lipid-rich domains in the bone tissue, providing an assessment of the composite's bioactivity.
26277184	4	29	from	domains	684:690	arg1	tissue					704:709	the bone tissue	695:709	the bone tissue	695:709	Using spectroscopic and chemometric methods, we were able to determine the presence of amides and phosphates and the distribution of lipid-rich domains in the bone tissue, providing an assessment of the composite's bioactivity.
26277184	2	30	theme	bone	418:421	arg1	process					430:436	the bone repair process	414:436	the bone repair process	414:436	The composite is an artificial bone material with an apatite-forming ability for the bone repair process.
26277184	1	31	theme	bone	308:311	arg1	engineering					320:330	bone tissue engineering	308:330	bone tissue engineering	308:330	The spectroscopic approaches of FTIR imaging and Raman mapping were applied to the characterisation of a new carbon hydroxyapatite/β-glucan composite developed for bone tissue engineering.
26277184	6	32	theme	spectral	947:954	arg1	quality					956:962	spectral quality	947:962	spectral quality	947:962	CaF2 lenses were also used on the infrared microscope to improve spectral quality by reducing scattering artefacts, improving chemometric analysis.
26277184	0	33	theme	carbon	24:29	arg1	composite					55:63	carbon hydroxyapatite/β-glucan composite	24:63	carbon hydroxyapatite/β-glucan composite	24:63	The biocompatibility of carbon hydroxyapatite/β-glucan composite for bone tissue engineering studied with Raman and FTIR spectroscopic imaging.
26277184	1	34	theme	tissue	313:318	arg1	engineering					320:330	bone tissue engineering	308:330	bone tissue engineering	308:330	The spectroscopic approaches of FTIR imaging and Raman mapping were applied to the characterisation of a new carbon hydroxyapatite/β-glucan composite developed for bone tissue engineering.
26277184	7	35	theme	microscopic	1165:1175	arg1	imaging					1177:1183	FTIR microscopic imaging	1160:1183	FTIR microscopic imaging	1160:1183	The presence of collagen and lipids at the bone/composite interface confirmed biocompatibility and demonstrate the suitability of FTIR microscopic imaging with lenses in studying these samples.
26277184	9	36	theme	FTIR	1488:1491	arg1	imaging					1507:1513	FTIR spectroscopic imaging	1488:1513	FTIR spectroscopic imaging	1488:1513	The results indicate the bioactive and biocompatible properties of this composite and demonstrate how Raman and FTIR spectroscopic imaging have been used as an effective tool for tissue characterisation.
26277184	9	36	theme	FTIR	1488:1491	arg1	Raman					1478:1482	Raman	1478:1482	Raman	1478:1482	The results indicate the bioactive and biocompatible properties of this composite and demonstrate how Raman and FTIR spectroscopic imaging have been used as an effective tool for tissue characterisation.
26277184	9	36	theme	FTIR	1488:1491	arg1	tool					1546:1549	an effective tool	1533:1549	an effective tool for tissue characterisation	1533:1577	The results indicate the bioactive and biocompatible properties of this composite and demonstrate how Raman and FTIR spectroscopic imaging have been used as an effective tool for tissue characterisation.
26277184	7	37	attach	presence	1034:1041	arg2	lipids					1059:1064	lipids	1059:1064	lipids	1059:1064	The presence of collagen and lipids at the bone/composite interface confirmed biocompatibility and demonstrate the suitability of FTIR microscopic imaging with lenses in studying these samples.
26277184	7	37	attach	presence	1034:1041	arg2	collagen					1046:1053	collagen	1046:1053	collagen	1046:1053	The presence of collagen and lipids at the bone/composite interface confirmed biocompatibility and demonstrate the suitability of FTIR microscopic imaging with lenses in studying these samples.
26277184	7	37	attach	presence	1034:1041	arg1	interface					1088:1096	the bone/composite interface	1069:1096	the bone/composite interface	1069:1096	The presence of collagen and lipids at the bone/composite interface confirmed biocompatibility and demonstrate the suitability of FTIR microscopic imaging with lenses in studying these samples.
26277184	5	38	theme	focal	854:858	arg1	detector					872:879	a focal plane array detector	852:879	a focal plane array detector	852:879	Samples were also imaged in transmission using an infrared microscope combined with a focal plane array detector.
26277184	8	39	theme	process	1367:1373	arg1	time					1343:1346	the time	1339:1346	the time of the bone growth process	1339:1373	It confirmed that the composite is a very good background for collagen growth and increases collagen maturity with the time of the bone growth process.
26277184	4	40	from	distribution	657:668	arg1	tissue					704:709	the bone tissue	695:709	the bone tissue	695:709	Using spectroscopic and chemometric methods, we were able to determine the presence of amides and phosphates and the distribution of lipid-rich domains in the bone tissue, providing an assessment of the composite's bioactivity.
26277184	5	41	theme	plane	860:864	arg1	detector					872:879	a focal plane array detector	852:879	a focal plane array detector	852:879	Samples were also imaged in transmission using an infrared microscope combined with a focal plane array detector.
26277184	0	42	theme	composite	55:63	arg1	biocompatibility					4:19	The biocompatibility	0:19	The biocompatibility of carbon hydroxyapatite/β-glucan composite for bone tissue engineering	0:91	The biocompatibility of carbon hydroxyapatite/β-glucan composite for bone tissue engineering studied with Raman and FTIR spectroscopic imaging.
26277184	4	43	theme	bone	699:702	arg1	tissue					704:709	the bone tissue	695:709	the bone tissue	695:709	Using spectroscopic and chemometric methods, we were able to determine the presence of amides and phosphates and the distribution of lipid-rich domains in the bone tissue, providing an assessment of the composite's bioactivity.
26277184	4	44	theme	lipid-rich	673:682	arg1	domains					684:690	lipid-rich domains	673:690	lipid-rich domains in the bone tissue	673:709	Using spectroscopic and chemometric methods, we were able to determine the presence of amides and phosphates and the distribution of lipid-rich domains in the bone tissue, providing an assessment of the composite's bioactivity.
26277184	3	45	theme	implanted	479:487	arg1	material					499:506	an implanted bioactive material	476:506	an implanted bioactive material for a period of several months	476:537	Rabbit bone samples were tested with an implanted bioactive material for a period of several months.
26277184	0	46	theme	hydroxyapatite/β-glucan	31:53	arg1	composite					55:63	carbon hydroxyapatite/β-glucan composite	24:63	carbon hydroxyapatite/β-glucan composite	24:63	The biocompatibility of carbon hydroxyapatite/β-glucan composite for bone tissue engineering studied with Raman and FTIR spectroscopic imaging.
26277184	4	47	theme	chemometric	564:574	arg1	methods					576:582	spectroscopic and chemometric methods	546:582	spectroscopic and chemometric methods	546:582	Using spectroscopic and chemometric methods, we were able to determine the presence of amides and phosphates and the distribution of lipid-rich domains in the bone tissue, providing an assessment of the composite's bioactivity.
26277184	4	48	from	presence	615:622	arg1	tissue					704:709	the bone tissue	695:709	the bone tissue	695:709	Using spectroscopic and chemometric methods, we were able to determine the presence of amides and phosphates and the distribution of lipid-rich domains in the bone tissue, providing an assessment of the composite's bioactivity.
26277184	9	49	theme	composite	1448:1456	arg1	properties					1429:1438	the bioactive and biocompatible properties	1397:1438	the bioactive and biocompatible properties of this composite	1397:1456	The results indicate the bioactive and biocompatible properties of this composite and demonstrate how Raman and FTIR spectroscopic imaging have been used as an effective tool for tissue characterisation.
26277184	7	50	theme	collagen	1046:1053	arg1	presence					1034:1041	The presence	1030:1041	The presence of collagen and lipids at the bone/composite interface	1030:1096	The presence of collagen and lipids at the bone/composite interface confirmed biocompatibility and demonstrate the suitability of FTIR microscopic imaging with lenses in studying these samples.
26277184	3	51	theme	bioactive	489:497	arg1	material					499:506	an implanted bioactive material	476:506	an implanted bioactive material for a period of several months	476:537	Rabbit bone samples were tested with an implanted bioactive material for a period of several months.
26277184	0	52	theme	bone	69:72	arg1	engineering					81:91	bone tissue engineering	69:91	bone tissue engineering	69:91	The biocompatibility of carbon hydroxyapatite/β-glucan composite for bone tissue engineering studied with Raman and FTIR spectroscopic imaging.
26277184	3	53	theme	months	532:537	arg1	period					514:519	a period	512:519	a period of several months	512:537	Rabbit bone samples were tested with an implanted bioactive material for a period of several months.
26277184	5	54	theme	infrared	818:825	arg1	microscope					827:836	an infrared microscope	815:836	an infrared microscope combined with a focal plane array detector	815:879	Samples were also imaged in transmission using an infrared microscope combined with a focal plane array detector.
26277184	2	55	with	material	369:376	arg1	ability					402:408	an apatite-forming ability	383:408	an apatite-forming ability for the bone repair process	383:436	The composite is an artificial bone material with an apatite-forming ability for the bone repair process.
26277184	7	56	from	interface	1088:1096	arg1	presence					1034:1041	The presence	1030:1041	The presence of collagen and lipids at the bone/composite interface	1030:1096	The presence of collagen and lipids at the bone/composite interface confirmed biocompatibility and demonstrate the suitability of FTIR microscopic imaging with lenses in studying these samples.
26277184	3	57	theme	Rabbit	439:444	arg1	samples					451:457	Rabbit bone samples	439:457	Rabbit bone samples	439:457	Rabbit bone samples were tested with an implanted bioactive material for a period of several months.
26277184	9	58	theme	biocompatible	1415:1427	arg1	properties					1429:1438	the bioactive and biocompatible properties	1397:1438	the bioactive and biocompatible properties of this composite	1397:1456	The results indicate the bioactive and biocompatible properties of this composite and demonstrate how Raman and FTIR spectroscopic imaging have been used as an effective tool for tissue characterisation.
26277184	7	59	theme	bone/composite	1073:1086	arg1	interface					1088:1096	the bone/composite interface	1069:1096	the bone/composite interface	1069:1096	The presence of collagen and lipids at the bone/composite interface confirmed biocompatibility and demonstrate the suitability of FTIR microscopic imaging with lenses in studying these samples.
26277184	9	60	theme	effective	1536:1544	arg1	imaging					1507:1513	FTIR spectroscopic imaging	1488:1513	FTIR spectroscopic imaging	1488:1513	The results indicate the bioactive and biocompatible properties of this composite and demonstrate how Raman and FTIR spectroscopic imaging have been used as an effective tool for tissue characterisation.
26277184	9	60	theme	effective	1536:1544	arg1	Raman					1478:1482	Raman	1478:1482	Raman	1478:1482	The results indicate the bioactive and biocompatible properties of this composite and demonstrate how Raman and FTIR spectroscopic imaging have been used as an effective tool for tissue characterisation.
26277184	9	60	theme	effective	1536:1544	arg1	tool					1546:1549	an effective tool	1533:1549	an effective tool for tissue characterisation	1533:1577	The results indicate the bioactive and biocompatible properties of this composite and demonstrate how Raman and FTIR spectroscopic imaging have been used as an effective tool for tissue characterisation.
26277184	3	61	theme	several	524:530	arg1	months					532:537	several months	524:537	several months	524:537	Rabbit bone samples were tested with an implanted bioactive material for a period of several months.
26277184	5	62	theme	array	866:870	arg1	detector					872:879	a focal plane array detector	852:879	a focal plane array detector	852:879	Samples were also imaged in transmission using an infrared microscope combined with a focal plane array detector.
26277184	7	63	theme	lipids	1059:1064	arg1	presence					1034:1041	The presence	1030:1041	The presence of collagen and lipids at the bone/composite interface	1030:1096	The presence of collagen and lipids at the bone/composite interface confirmed biocompatibility and demonstrate the suitability of FTIR microscopic imaging with lenses in studying these samples.
26277184	7	64	with	suitability	1145:1155	arg1	lenses					1190:1195	lenses	1190:1195	lenses in studying these samples	1190:1221	The presence of collagen and lipids at the bone/composite interface confirmed biocompatibility and demonstrate the suitability of FTIR microscopic imaging with lenses in studying these samples.
26277184	2	65	theme	bone	364:367	arg1	material					369:376	an artificial bone material	350:376	an artificial bone material with an apatite-forming ability for the bone repair process	350:436	The composite is an artificial bone material with an apatite-forming ability for the bone repair process.
26277184	2	65	theme	bone	364:367	arg1	composite					337:345	The composite	333:345	The composite	333:345	The composite is an artificial bone material with an apatite-forming ability for the bone repair process.
26277184	9	66	theme	spectroscopic	1493:1505	arg1	imaging					1507:1513	FTIR spectroscopic imaging	1488:1513	FTIR spectroscopic imaging	1488:1513	The results indicate the bioactive and biocompatible properties of this composite and demonstrate how Raman and FTIR spectroscopic imaging have been used as an effective tool for tissue characterisation.
26277184	9	66	theme	spectroscopic	1493:1505	arg1	Raman					1478:1482	Raman	1478:1482	Raman	1478:1482	The results indicate the bioactive and biocompatible properties of this composite and demonstrate how Raman and FTIR spectroscopic imaging have been used as an effective tool for tissue characterisation.
26277184	9	66	theme	spectroscopic	1493:1505	arg1	tool					1546:1549	an effective tool	1533:1549	an effective tool for tissue characterisation	1533:1577	The results indicate the bioactive and biocompatible properties of this composite and demonstrate how Raman and FTIR spectroscopic imaging have been used as an effective tool for tissue characterisation.
26277184	1	67	theme	spectroscopic	148:160	arg1	approaches					162:171	The spectroscopic approaches	144:171	The spectroscopic approaches of FTIR imaging and Raman mapping	144:205	The spectroscopic approaches of FTIR imaging and Raman mapping were applied to the characterisation of a new carbon hydroxyapatite/β-glucan composite developed for bone tissue engineering.
26277184	6	68	dep	reducing	967:974	arg1	improving					998:1006	improving	998:1006	improving chemometric analysis	998:1027	CaF2 lenses were also used on the infrared microscope to improve spectral quality by reducing scattering artefacts, improving chemometric analysis.
26277184	4	69	theme	amides	627:632	arg1	presence					615:622	the presence	611:622	the presence of amides and phosphates	611:647	Using spectroscopic and chemometric methods, we were able to determine the presence of amides and phosphates and the distribution of lipid-rich domains in the bone tissue, providing an assessment of the composite's bioactivity.
26277184	4	69	theme	amides	627:632	arg1	distribution					657:668	the distribution	653:668	the distribution of lipid-rich domains in the bone tissue	653:709	Using spectroscopic and chemometric methods, we were able to determine the presence of amides and phosphates and the distribution of lipid-rich domains in the bone tissue, providing an assessment of the composite's bioactivity.
26277184	8	70	theme	collagen	1316:1323	arg1	maturity					1325:1332	collagen maturity	1316:1332	collagen maturity with the time of the bone growth process	1316:1373	It confirmed that the composite is a very good background for collagen growth and increases collagen maturity with the time of the bone growth process.
26277184	8	71	theme	growth	1360:1365	arg1	process					1367:1373	the bone growth process	1351:1373	the bone growth process	1351:1373	It confirmed that the composite is a very good background for collagen growth and increases collagen maturity with the time of the bone growth process.
26277184	2	72	theme	repair	423:428	arg1	process					430:436	the bone repair process	414:436	the bone repair process	414:436	The composite is an artificial bone material with an apatite-forming ability for the bone repair process.
26277184	1	73	theme	new	249:251	arg1	composite					284:292	a new carbon hydroxyapatite/β-glucan composite	247:292	a new carbon hydroxyapatite/β-glucan composite developed for bone tissue engineering	247:330	The spectroscopic approaches of FTIR imaging and Raman mapping were applied to the characterisation of a new carbon hydroxyapatite/β-glucan composite developed for bone tissue engineering.
26277184	0	74	theme	tissue	74:79	arg1	engineering					81:91	bone tissue engineering	69:91	bone tissue engineering	69:91	The biocompatibility of carbon hydroxyapatite/β-glucan composite for bone tissue engineering studied with Raman and FTIR spectroscopic imaging.
26277184	4	75	theme	phosphates	638:647	arg1	presence					615:622	the presence	611:622	the presence of amides and phosphates	611:647	Using spectroscopic and chemometric methods, we were able to determine the presence of amides and phosphates and the distribution of lipid-rich domains in the bone tissue, providing an assessment of the composite's bioactivity.
26277184	4	75	theme	phosphates	638:647	arg1	distribution					657:668	the distribution	653:668	the distribution of lipid-rich domains in the bone tissue	653:709	Using spectroscopic and chemometric methods, we were able to determine the presence of amides and phosphates and the distribution of lipid-rich domains in the bone tissue, providing an assessment of the composite's bioactivity.
26277184	8	76	theme	bone	1355:1358	arg1	process					1367:1373	the bone growth process	1351:1373	the bone growth process	1351:1373	It confirmed that the composite is a very good background for collagen growth and increases collagen maturity with the time of the bone growth process.
26277184	1	77	theme	carbon	253:258	arg1	composite					284:292	a new carbon hydroxyapatite/β-glucan composite	247:292	a new carbon hydroxyapatite/β-glucan composite developed for bone tissue engineering	247:330	The spectroscopic approaches of FTIR imaging and Raman mapping were applied to the characterisation of a new carbon hydroxyapatite/β-glucan composite developed for bone tissue engineering.
25505137	0	0	from	profiling	12:20	arg1	cereus					59:64	Bacillus cereus	50:64	Bacillus cereus	50:64	Single cell profiling of surface carbohydrates on Bacillus cereus.
25505137	4	1	from	spore	779:783	arg1	compositions					738:749	the surface carbohydrate compositions	713:749	the surface carbohydrate compositions from the vegetative cell to spore	713:783	Further, the changes of the surface carbohydrate compositions from the vegetative cell to spore were shown.
25505137	4	1	from	spore	779:783	arg1	changes					702:708	the changes	698:708	the changes of the surface carbohydrate compositions from the vegetative cell to spore	698:783	Further, the changes of the surface carbohydrate compositions from the vegetative cell to spore were shown.
25505137	2	2	theme	cell	353:356	arg1	level					358:362	the single cell level	342:362	the single cell level	342:362	It is well known that different types of Bacillus display heterogeneity of surface carbohydrate compositions, but detection of their presence, quantitation and estimation of variation at the single cell level have not been previously solved.
25505137	4	3	theme	cell	771:774	arg1	spore					779:783	the vegetative cell to spore	756:783	the vegetative cell to spore	756:783	Further, the changes of the surface carbohydrate compositions from the vegetative cell to spore were shown.
25505137	5	4	theme	surface	942:948	arg1	biomarkers					950:959	key bacterial surface biomarkers	928:959	key bacterial surface biomarkers (such as carbohydrate compositions)	928:995	These results demonstrate AFM-based 'recognition force mapping' as a versatile platform to quantitatively detect and spatially map key bacterial surface biomarkers (such as carbohydrate compositions), and monitor in situ changes in surface biochemical properties during intracellular activities at the single cell level.
25505137	5	5	theme	cell	1106:1109	arg1	level					1111:1115	the single cell level	1095:1115	the single cell level	1095:1115	These results demonstrate AFM-based 'recognition force mapping' as a versatile platform to quantitatively detect and spatially map key bacterial surface biomarkers (such as carbohydrate compositions), and monitor in situ changes in surface biochemical properties during intracellular activities at the single cell level.
25505137	3	6	theme	N-acetylglucosamine	542:560	arg1	distributions					525:537	the specific carbohydrate distributions	499:537	the specific carbohydrate distributions of N-acetylglucosamine and mannose/glucose	499:580	Here, using atomic force microscopy (AFM)-based recognition force mapping coupled with lectin probes, the specific carbohydrate distributions of N-acetylglucosamine and mannose/glucose were detected, mapped and quantified on single B. cereus surfaces at the nanoscale across the entire cell.
25505137	2	7	theme	presence	288:295	arg1	estimation					315:324	estimation	315:324	estimation	315:324	It is well known that different types of Bacillus display heterogeneity of surface carbohydrate compositions, but detection of their presence, quantitation and estimation of variation at the single cell level have not been previously solved.
25505137	2	7	theme	presence	288:295	arg1	quantitation					298:309	quantitation	298:309	quantitation	298:309	It is well known that different types of Bacillus display heterogeneity of surface carbohydrate compositions, but detection of their presence, quantitation and estimation of variation at the single cell level have not been previously solved.
25505137	2	7	theme	presence	288:295	arg1	detection					269:277	detection	269:277	detection of their presence	269:295	It is well known that different types of Bacillus display heterogeneity of surface carbohydrate compositions, but detection of their presence, quantitation and estimation of variation at the single cell level have not been previously solved.
25505137	5	8	theme	bacterial	932:940	arg1	biomarkers					950:959	key bacterial surface biomarkers	928:959	key bacterial surface biomarkers (such as carbohydrate compositions)	928:995	These results demonstrate AFM-based 'recognition force mapping' as a versatile platform to quantitatively detect and spatially map key bacterial surface biomarkers (such as carbohydrate compositions), and monitor in situ changes in surface biochemical properties during intracellular activities at the single cell level.
25505137	1	9	theme	various	111:117	arg1	activities					129:138	various bacterial activities	111:138	various bacterial activities	111:138	Cell surface carbohydrates are important to various bacterial activities and functions.
25505137	5	10	theme	force	846:850	arg1	mapping					852:858	AFM-based 'recognition force mapping'	823:859	AFM-based 'recognition force mapping'	823:859	These results demonstrate AFM-based 'recognition force mapping' as a versatile platform to quantitatively detect and spatially map key bacterial surface biomarkers (such as carbohydrate compositions), and monitor in situ changes in surface biochemical properties during intracellular activities at the single cell level.
25505137	5	10	theme	force	846:850	arg1	platform					876:883	a versatile platform	864:883	a versatile platform to quantitatively detect and spatially map key bacterial surface biomarkers (such as carbohydrate compositions), and monitor in situ changes in surface biochemical properties during intracellular activities at the single cell level	864:1115	These results demonstrate AFM-based 'recognition force mapping' as a versatile platform to quantitatively detect and spatially map key bacterial surface biomarkers (such as carbohydrate compositions), and monitor in situ changes in surface biochemical properties during intracellular activities at the single cell level.
25505137	1	11	theme	Cell	67:70	arg1	carbohydrates					80:92	Cell surface carbohydrates	67:92	Cell surface carbohydrates	67:92	Cell surface carbohydrates are important to various bacterial activities and functions.
25505137	5	12	theme	versatile	866:874	arg1	mapping					852:858	AFM-based 'recognition force mapping'	823:859	AFM-based 'recognition force mapping'	823:859	These results demonstrate AFM-based 'recognition force mapping' as a versatile platform to quantitatively detect and spatially map key bacterial surface biomarkers (such as carbohydrate compositions), and monitor in situ changes in surface biochemical properties during intracellular activities at the single cell level.
25505137	5	12	theme	versatile	866:874	arg1	platform					876:883	a versatile platform	864:883	a versatile platform to quantitatively detect and spatially map key bacterial surface biomarkers (such as carbohydrate compositions), and monitor in situ changes in surface biochemical properties during intracellular activities at the single cell level	864:1115	These results demonstrate AFM-based 'recognition force mapping' as a versatile platform to quantitatively detect and spatially map key bacterial surface biomarkers (such as carbohydrate compositions), and monitor in situ changes in surface biochemical properties during intracellular activities at the single cell level.
25505137	3	13	from	nanoscale	655:663	arg1	surfaces					639:646	single B. cereus surfaces	622:646	single B. cereus surfaces at the nanoscale across the entire cell	622:686	Here, using atomic force microscopy (AFM)-based recognition force mapping coupled with lectin probes, the specific carbohydrate distributions of N-acetylglucosamine and mannose/glucose were detected, mapped and quantified on single B. cereus surfaces at the nanoscale across the entire cell.
25505137	2	14	from	level	358:362	arg1	estimation					315:324	estimation	315:324	estimation	315:324	It is well known that different types of Bacillus display heterogeneity of surface carbohydrate compositions, but detection of their presence, quantitation and estimation of variation at the single cell level have not been previously solved.
25505137	2	14	from	level	358:362	arg1	quantitation					298:309	quantitation	298:309	quantitation	298:309	It is well known that different types of Bacillus display heterogeneity of surface carbohydrate compositions, but detection of their presence, quantitation and estimation of variation at the single cell level have not been previously solved.
25505137	2	14	from	level	358:362	arg1	detection					269:277	detection	269:277	detection of their presence	269:295	It is well known that different types of Bacillus display heterogeneity of surface carbohydrate compositions, but detection of their presence, quantitation and estimation of variation at the single cell level have not been previously solved.
25505137	1	15	theme	surface	72:78	arg1	carbohydrates					80:92	Cell surface carbohydrates	67:92	Cell surface carbohydrates	67:92	Cell surface carbohydrates are important to various bacterial activities and functions.
25505137	0	16	theme	cell	7:10	arg1	profiling					12:20	Single cell profiling	0:20	Single cell profiling of surface carbohydrates on Bacillus cereus	0:64	Single cell profiling of surface carbohydrates on Bacillus cereus.
25505137	4	17	theme	compositions	738:749	arg1	changes					702:708	the changes	698:708	the changes of the surface carbohydrate compositions from the vegetative cell to spore	698:783	Further, the changes of the surface carbohydrate compositions from the vegetative cell to spore were shown.
25505137	3	18	theme	force	416:420	arg1	AFM					434:436	AFM	434:436	AFM	434:436	Here, using atomic force microscopy (AFM)-based recognition force mapping coupled with lectin probes, the specific carbohydrate distributions of N-acetylglucosamine and mannose/glucose were detected, mapped and quantified on single B. cereus surfaces at the nanoscale across the entire cell.
25505137	3	18	theme	force	416:420	arg1	microscopy					422:431	atomic force microscopy	409:431	atomic force microscopy (AFM)	409:437	Here, using atomic force microscopy (AFM)-based recognition force mapping coupled with lectin probes, the specific carbohydrate distributions of N-acetylglucosamine and mannose/glucose were detected, mapped and quantified on single B. cereus surfaces at the nanoscale across the entire cell.
25505137	0	19	theme	Single	0:5	arg1	profiling					12:20	Single cell profiling	0:20	Single cell profiling of surface carbohydrates on Bacillus cereus	0:64	Single cell profiling of surface carbohydrates on Bacillus cereus.
25505137	4	20	theme	carbohydrate	725:736	arg1	compositions					738:749	the surface carbohydrate compositions	713:749	the surface carbohydrate compositions from the vegetative cell to spore	713:783	Further, the changes of the surface carbohydrate compositions from the vegetative cell to spore were shown.
25505137	5	21	theme	AFM-based	823:831	arg1	mapping					852:858	AFM-based 'recognition force mapping'	823:859	AFM-based 'recognition force mapping'	823:859	These results demonstrate AFM-based 'recognition force mapping' as a versatile platform to quantitatively detect and spatially map key bacterial surface biomarkers (such as carbohydrate compositions), and monitor in situ changes in surface biochemical properties during intracellular activities at the single cell level.
25505137	5	21	theme	AFM-based	823:831	arg1	platform					876:883	a versatile platform	864:883	a versatile platform to quantitatively detect and spatially map key bacterial surface biomarkers (such as carbohydrate compositions), and monitor in situ changes in surface biochemical properties during intracellular activities at the single cell level	864:1115	These results demonstrate AFM-based 'recognition force mapping' as a versatile platform to quantitatively detect and spatially map key bacterial surface biomarkers (such as carbohydrate compositions), and monitor in situ changes in surface biochemical properties during intracellular activities at the single cell level.
25505137	5	22	theme	biochemical	1037:1047	arg1	properties					1049:1058	surface biochemical properties	1029:1058	surface biochemical properties	1029:1058	These results demonstrate AFM-based 'recognition force mapping' as a versatile platform to quantitatively detect and spatially map key bacterial surface biomarkers (such as carbohydrate compositions), and monitor in situ changes in surface biochemical properties during intracellular activities at the single cell level.
25505137	5	23	theme	situ	1013:1016	arg1	changes					1018:1024	situ changes	1013:1024	situ changes in surface biochemical properties	1013:1058	These results demonstrate AFM-based 'recognition force mapping' as a versatile platform to quantitatively detect and spatially map key bacterial surface biomarkers (such as carbohydrate compositions), and monitor in situ changes in surface biochemical properties during intracellular activities at the single cell level.
25505137	3	24	theme	recognition	445:455	arg1	mapping					463:469	atomic force microscopy (AFM)-based recognition force mapping	409:469	atomic force microscopy (AFM)-based recognition force mapping coupled with lectin probes	409:496	Here, using atomic force microscopy (AFM)-based recognition force mapping coupled with lectin probes, the specific carbohydrate distributions of N-acetylglucosamine and mannose/glucose were detected, mapped and quantified on single B. cereus surfaces at the nanoscale across the entire cell.
25505137	5	25	from	level	1111:1115	arg1	activities					1081:1090	intracellular activities	1067:1090	intracellular activities at the single cell level	1067:1115	These results demonstrate AFM-based 'recognition force mapping' as a versatile platform to quantitatively detect and spatially map key bacterial surface biomarkers (such as carbohydrate compositions), and monitor in situ changes in surface biochemical properties during intracellular activities at the single cell level.
25505137	3	26	theme	carbohydrate	512:523	arg1	distributions					525:537	the specific carbohydrate distributions	499:537	the specific carbohydrate distributions of N-acetylglucosamine and mannose/glucose	499:580	Here, using atomic force microscopy (AFM)-based recognition force mapping coupled with lectin probes, the specific carbohydrate distributions of N-acetylglucosamine and mannose/glucose were detected, mapped and quantified on single B. cereus surfaces at the nanoscale across the entire cell.
25505137	2	27	theme	different	177:185	arg1	types					187:191	different types	177:191	different types of Bacillus	177:203	It is well known that different types of Bacillus display heterogeneity of surface carbohydrate compositions, but detection of their presence, quantitation and estimation of variation at the single cell level have not been previously solved.
25505137	3	28	theme	mannose/glucose	566:580	arg1	distributions					525:537	the specific carbohydrate distributions	499:537	the specific carbohydrate distributions of N-acetylglucosamine and mannose/glucose	499:580	Here, using atomic force microscopy (AFM)-based recognition force mapping coupled with lectin probes, the specific carbohydrate distributions of N-acetylglucosamine and mannose/glucose were detected, mapped and quantified on single B. cereus surfaces at the nanoscale across the entire cell.
25505137	3	29	theme	force	457:461	arg1	mapping					463:469	atomic force microscopy (AFM)-based recognition force mapping	409:469	atomic force microscopy (AFM)-based recognition force mapping coupled with lectin probes	409:496	Here, using atomic force microscopy (AFM)-based recognition force mapping coupled with lectin probes, the specific carbohydrate distributions of N-acetylglucosamine and mannose/glucose were detected, mapped and quantified on single B. cereus surfaces at the nanoscale across the entire cell.
25505137	0	30	theme	carbohydrates	33:45	arg1	profiling					12:20	Single cell profiling	0:20	Single cell profiling of surface carbohydrates on Bacillus cereus	0:64	Single cell profiling of surface carbohydrates on Bacillus cereus.
25505137	3	31	theme	lectin	484:489	arg1	probes					491:496	lectin probes	484:496	lectin probes	484:496	Here, using atomic force microscopy (AFM)-based recognition force mapping coupled with lectin probes, the specific carbohydrate distributions of N-acetylglucosamine and mannose/glucose were detected, mapped and quantified on single B. cereus surfaces at the nanoscale across the entire cell.
25505137	2	32	theme	carbohydrate	238:249	arg1	compositions					251:262	surface carbohydrate compositions	230:262	surface carbohydrate compositions	230:262	It is well known that different types of Bacillus display heterogeneity of surface carbohydrate compositions, but detection of their presence, quantitation and estimation of variation at the single cell level have not been previously solved.
25505137	5	33	from	changes	1018:1024	arg1	properties					1049:1058	surface biochemical properties	1029:1058	surface biochemical properties	1029:1058	These results demonstrate AFM-based 'recognition force mapping' as a versatile platform to quantitatively detect and spatially map key bacterial surface biomarkers (such as carbohydrate compositions), and monitor in situ changes in surface biochemical properties during intracellular activities at the single cell level.
25505137	3	34	dep	B.	629:630	arg1	cereus					632:637	cereus	632:637	cereus	632:637	Here, using atomic force microscopy (AFM)-based recognition force mapping coupled with lectin probes, the specific carbohydrate distributions of N-acetylglucosamine and mannose/glucose were detected, mapped and quantified on single B. cereus surfaces at the nanoscale across the entire cell.
25505137	0	35	theme	surface	25:31	arg1	carbohydrates					33:45	surface carbohydrates	25:45	surface carbohydrates	25:45	Single cell profiling of surface carbohydrates on Bacillus cereus.
25505137	3	36	theme	atomic	409:414	arg1	AFM					434:436	AFM	434:436	AFM	434:436	Here, using atomic force microscopy (AFM)-based recognition force mapping coupled with lectin probes, the specific carbohydrate distributions of N-acetylglucosamine and mannose/glucose were detected, mapped and quantified on single B. cereus surfaces at the nanoscale across the entire cell.
25505137	3	36	theme	atomic	409:414	arg1	microscopy					422:431	atomic force microscopy	409:431	atomic force microscopy (AFM)	409:437	Here, using atomic force microscopy (AFM)-based recognition force mapping coupled with lectin probes, the specific carbohydrate distributions of N-acetylglucosamine and mannose/glucose were detected, mapped and quantified on single B. cereus surfaces at the nanoscale across the entire cell.
25505137	4	37	theme	vegetative	760:769	arg1	spore					779:783	the vegetative cell to spore	756:783	the vegetative cell to spore	756:783	Further, the changes of the surface carbohydrate compositions from the vegetative cell to spore were shown.
25505137	2	38	theme	surface	230:236	arg1	compositions					251:262	surface carbohydrate compositions	230:262	surface carbohydrate compositions	230:262	It is well known that different types of Bacillus display heterogeneity of surface carbohydrate compositions, but detection of their presence, quantitation and estimation of variation at the single cell level have not been previously solved.
25505137	3	39	theme	-based	438:443	arg1	mapping					463:469	atomic force microscopy (AFM)-based recognition force mapping	409:469	atomic force microscopy (AFM)-based recognition force mapping coupled with lectin probes	409:496	Here, using atomic force microscopy (AFM)-based recognition force mapping coupled with lectin probes, the specific carbohydrate distributions of N-acetylglucosamine and mannose/glucose were detected, mapped and quantified on single B. cereus surfaces at the nanoscale across the entire cell.
25505137	2	40	gly	heterogeneity	213:225	arg1	compositions					251:262	surface carbohydrate compositions	230:262	surface carbohydrate compositions	230:262	It is well known that different types of Bacillus display heterogeneity of surface carbohydrate compositions, but detection of their presence, quantitation and estimation of variation at the single cell level have not been previously solved.
25505137	5	41	theme	single	1099:1104	arg1	level					1111:1115	the single cell level	1095:1115	the single cell level	1095:1115	These results demonstrate AFM-based 'recognition force mapping' as a versatile platform to quantitatively detect and spatially map key bacterial surface biomarkers (such as carbohydrate compositions), and monitor in situ changes in surface biochemical properties during intracellular activities at the single cell level.
25505137	5	42	theme	carbohydrate	970:981	arg1	compositions					983:994	carbohydrate compositions	970:994	carbohydrate compositions	970:994	These results demonstrate AFM-based 'recognition force mapping' as a versatile platform to quantitatively detect and spatially map key bacterial surface biomarkers (such as carbohydrate compositions), and monitor in situ changes in surface biochemical properties during intracellular activities at the single cell level.
25505137	2	43	theme	single	346:351	arg1	level					358:362	the single cell level	342:362	the single cell level	342:362	It is well known that different types of Bacillus display heterogeneity of surface carbohydrate compositions, but detection of their presence, quantitation and estimation of variation at the single cell level have not been previously solved.
25505137	5	44	dep	biomarkers	950:959	arg1	such					962:965	such	962:965	such	962:965	These results demonstrate AFM-based 'recognition force mapping' as a versatile platform to quantitatively detect and spatially map key bacterial surface biomarkers (such as carbohydrate compositions), and monitor in situ changes in surface biochemical properties during intracellular activities at the single cell level.
25505137	3	45	theme	entire	676:681	arg1	cell					683:686	the entire cell	672:686	the entire cell	672:686	Here, using atomic force microscopy (AFM)-based recognition force mapping coupled with lectin probes, the specific carbohydrate distributions of N-acetylglucosamine and mannose/glucose were detected, mapped and quantified on single B. cereus surfaces at the nanoscale across the entire cell.
25505137	5	46	theme	surface	1029:1035	arg1	properties					1049:1058	surface biochemical properties	1029:1058	surface biochemical properties	1029:1058	These results demonstrate AFM-based 'recognition force mapping' as a versatile platform to quantitatively detect and spatially map key bacterial surface biomarkers (such as carbohydrate compositions), and monitor in situ changes in surface biochemical properties during intracellular activities at the single cell level.
25505137	1	47	theme	bacterial	119:127	arg1	activities					129:138	various bacterial activities	111:138	various bacterial activities	111:138	Cell surface carbohydrates are important to various bacterial activities and functions.
25505137	3	48	theme	single	622:627	arg1	surfaces					639:646	single B. cereus surfaces	622:646	single B. cereus surfaces at the nanoscale across the entire cell	622:686	Here, using atomic force microscopy (AFM)-based recognition force mapping coupled with lectin probes, the specific carbohydrate distributions of N-acetylglucosamine and mannose/glucose were detected, mapped and quantified on single B. cereus surfaces at the nanoscale across the entire cell.
25505137	2	49	theme	Bacillus	196:203	arg1	types					187:191	different types	177:191	different types of Bacillus	177:203	It is well known that different types of Bacillus display heterogeneity of surface carbohydrate compositions, but detection of their presence, quantitation and estimation of variation at the single cell level have not been previously solved.
25505137	3	50	theme	B.	629:630	arg1	surfaces					639:646	single B. cereus surfaces	622:646	single B. cereus surfaces at the nanoscale across the entire cell	622:686	Here, using atomic force microscopy (AFM)-based recognition force mapping coupled with lectin probes, the specific carbohydrate distributions of N-acetylglucosamine and mannose/glucose were detected, mapped and quantified on single B. cereus surfaces at the nanoscale across the entire cell.
25505137	4	51	theme	surface	717:723	arg1	compositions					738:749	the surface carbohydrate compositions	713:749	the surface carbohydrate compositions from the vegetative cell to spore	713:783	Further, the changes of the surface carbohydrate compositions from the vegetative cell to spore were shown.
25505137	2	52	theme	variation	329:337	arg1	estimation					315:324	estimation	315:324	estimation	315:324	It is well known that different types of Bacillus display heterogeneity of surface carbohydrate compositions, but detection of their presence, quantitation and estimation of variation at the single cell level have not been previously solved.
25505137	2	52	theme	variation	329:337	arg1	quantitation					298:309	quantitation	298:309	quantitation	298:309	It is well known that different types of Bacillus display heterogeneity of surface carbohydrate compositions, but detection of their presence, quantitation and estimation of variation at the single cell level have not been previously solved.
25505137	2	52	theme	variation	329:337	arg1	detection					269:277	detection	269:277	detection of their presence	269:295	It is well known that different types of Bacillus display heterogeneity of surface carbohydrate compositions, but detection of their presence, quantitation and estimation of variation at the single cell level have not been previously solved.
25505137	5	53	theme	key	928:930	arg1	biomarkers					950:959	key bacterial surface biomarkers	928:959	key bacterial surface biomarkers (such as carbohydrate compositions)	928:995	These results demonstrate AFM-based 'recognition force mapping' as a versatile platform to quantitatively detect and spatially map key bacterial surface biomarkers (such as carbohydrate compositions), and monitor in situ changes in surface biochemical properties during intracellular activities at the single cell level.
25505137	5	54	theme	intracellular	1067:1079	arg1	activities					1081:1090	intracellular activities	1067:1090	intracellular activities at the single cell level	1067:1115	These results demonstrate AFM-based 'recognition force mapping' as a versatile platform to quantitatively detect and spatially map key bacterial surface biomarkers (such as carbohydrate compositions), and monitor in situ changes in surface biochemical properties during intracellular activities at the single cell level.
25505137	5	55	theme	recognition	834:844	arg1	mapping					852:858	AFM-based 'recognition force mapping'	823:859	AFM-based 'recognition force mapping'	823:859	These results demonstrate AFM-based 'recognition force mapping' as a versatile platform to quantitatively detect and spatially map key bacterial surface biomarkers (such as carbohydrate compositions), and monitor in situ changes in surface biochemical properties during intracellular activities at the single cell level.
25505137	5	55	theme	recognition	834:844	arg1	platform					876:883	a versatile platform	864:883	a versatile platform to quantitatively detect and spatially map key bacterial surface biomarkers (such as carbohydrate compositions), and monitor in situ changes in surface biochemical properties during intracellular activities at the single cell level	864:1115	These results demonstrate AFM-based 'recognition force mapping' as a versatile platform to quantitatively detect and spatially map key bacterial surface biomarkers (such as carbohydrate compositions), and monitor in situ changes in surface biochemical properties during intracellular activities at the single cell level.
25505137	4	56	dep	spore	779:783	arg1	to					776:777	to	776:777	to	776:777	Further, the changes of the surface carbohydrate compositions from the vegetative cell to spore were shown.
25505137	4	57	from	changes	702:708	arg1	spore					779:783	the vegetative cell to spore	756:783	the vegetative cell to spore	756:783	Further, the changes of the surface carbohydrate compositions from the vegetative cell to spore were shown.
25505137	2	58	theme	compositions	251:262	arg1	heterogeneity					213:225	heterogeneity	213:225	heterogeneity of surface carbohydrate compositions	213:262	It is well known that different types of Bacillus display heterogeneity of surface carbohydrate compositions, but detection of their presence, quantitation and estimation of variation at the single cell level have not been previously solved.
25505137	3	59	theme	specific	503:510	arg1	distributions					525:537	the specific carbohydrate distributions	499:537	the specific carbohydrate distributions of N-acetylglucosamine and mannose/glucose	499:580	Here, using atomic force microscopy (AFM)-based recognition force mapping coupled with lectin probes, the specific carbohydrate distributions of N-acetylglucosamine and mannose/glucose were detected, mapped and quantified on single B. cereus surfaces at the nanoscale across the entire cell.
28732850	8	0	theme	equal	1066:1070	arg1	biocompatible					1111:1123	biocompatible	1111:1123	biocompatible	1111:1123	The composite containing equal parts PPy and chitosan was found to be biocompatible; together with the corrosion protection results, the findings indicated that this bioactive coating material has potential for use in 316L SS implants.
28732850	8	0	theme	equal	1066:1070	arg1	parts					1072:1076	The composite containing equal parts	1041:1076	The composite containing equal parts PPy and chitosan	1041:1093	The composite containing equal parts PPy and chitosan was found to be biocompatible; together with the corrosion protection results, the findings indicated that this bioactive coating material has potential for use in 316L SS implants.
28732850	8	0	theme	equal	1066:1070	arg1	chitosan					1086:1093	chitosan	1086:1093	chitosan	1086:1093	The composite containing equal parts PPy and chitosan was found to be biocompatible; together with the corrosion protection results, the findings indicated that this bioactive coating material has potential for use in 316L SS implants.
28732850	8	0	theme	equal	1066:1070	arg1	PPy					1078:1080	PPy	1078:1080	PPy	1078:1080	The composite containing equal parts PPy and chitosan was found to be biocompatible; together with the corrosion protection results, the findings indicated that this bioactive coating material has potential for use in 316L SS implants.
28732850	4	1	theme	surface	577:583	arg1	hydrophilicity					585:598	surface hydrophilicity	577:598	surface hydrophilicity due to the addition of chitosan into the PPy matrix	577:650	Contact angle results confirmed the enhancement in surface hydrophilicity due to the addition of chitosan into the PPy matrix.
28732850	0	2	theme	in	77:78	arg1	performance					103:113	in vitro biocompatibility performance	77:113	in vitro biocompatibility performance	77:113	Promising bio-composites of polypyrrole and chitosan: Surface protective and in vitro biocompatibility performance on 316L SS implants.
28732850	5	3	theme	enhanced	731:738	arg1	performance					751:761	enhanced protective performance	731:761	enhanced protective performance	731:761	Electrochemical corrosion studies revealed that the composite coatings showed enhanced protective performance compared to pure PPy.
28732850	8	4	theme	SS	1264:1265	arg1	implants					1267:1274	316L SS implants	1259:1274	316L SS implants	1259:1274	The composite containing equal parts PPy and chitosan was found to be biocompatible; together with the corrosion protection results, the findings indicated that this bioactive coating material has potential for use in 316L SS implants.
28732850	4	5	theme	due	600:602	arg1	hydrophilicity					585:598	surface hydrophilicity	577:598	surface hydrophilicity due to the addition of chitosan into the PPy matrix	577:650	Contact angle results confirmed the enhancement in surface hydrophilicity due to the addition of chitosan into the PPy matrix.
28732850	8	6	theme	corrosion	1144:1152	arg1	results					1165:1171	the corrosion protection results	1140:1171	the corrosion protection results	1140:1171	The composite containing equal parts PPy and chitosan was found to be biocompatible; together with the corrosion protection results, the findings indicated that this bioactive coating material has potential for use in 316L SS implants.
28732850	2	7	from	polymerization	369:382	arg1	medium					399:404	oxalic acid medium	387:404	oxalic acid medium	387:404	We synthesized bioactive composites based on polypyrrole/chitosan through in-situ electrochemical polymerization in oxalic acid medium.
28732850	0	8	theme	biocompatibility	86:101	arg1	performance					103:113	in vitro biocompatibility performance	77:113	in vitro biocompatibility performance	77:113	Promising bio-composites of polypyrrole and chitosan: Surface protective and in vitro biocompatibility performance on 316L SS implants.
28732850	5	9	theme	pure	775:778	arg1	PPy					780:782	pure PPy	775:782	pure PPy	775:782	Electrochemical corrosion studies revealed that the composite coatings showed enhanced protective performance compared to pure PPy.
28732850	2	10	theme	oxalic	387:392	arg1	acid					394:397	oxalic acid	387:397	oxalic acid medium	387:404	We synthesized bioactive composites based on polypyrrole/chitosan through in-situ electrochemical polymerization in oxalic acid medium.
28732850	0	11	theme	316L	118:121	arg1	implants					126:133	316L SS implants	118:133	316L SS implants	118:133	Promising bio-composites of polypyrrole and chitosan: Surface protective and in vitro biocompatibility performance on 316L SS implants.
28732850	3	12	from	influence	453:461	arg1	morphology					514:523	polypyrrole (PPy) surface morphology	488:523	polypyrrole (PPy) surface morphology	488:523	Surface characterization results revealed the influence of chitosan inclusion on polypyrrole (PPy) surface morphology.
28732850	5	13	theme	composite	705:713	arg1	coatings					715:722	the composite coatings	701:722	the composite coatings	701:722	Electrochemical corrosion studies revealed that the composite coatings showed enhanced protective performance compared to pure PPy.
28732850	6	14	from	effect	814:819	arg1	growth					854:859	the growth	850:859	the growth of MG-63 human osteoblast cells	850:891	Further, we investigated the effect of the composite coatings on the growth of MG-63 human osteoblast cells to assess their biocompatibility.
28732850	3	15	theme	surface	506:512	arg1	morphology					514:523	polypyrrole (PPy) surface morphology	488:523	polypyrrole (PPy) surface morphology	488:523	Surface characterization results revealed the influence of chitosan inclusion on polypyrrole (PPy) surface morphology.
28732850	3	16	theme	characterization	415:430	arg1	results					432:438	Surface characterization results	407:438	Surface characterization results	407:438	Surface characterization results revealed the influence of chitosan inclusion on polypyrrole (PPy) surface morphology.
28732850	3	17	theme	polypyrrole	488:498	arg1	morphology					514:523	polypyrrole (PPy) surface morphology	488:523	polypyrrole (PPy) surface morphology	488:523	Surface characterization results revealed the influence of chitosan inclusion on polypyrrole (PPy) surface morphology.
28732850	6	18	theme	cells	887:891	arg1	growth					854:859	the growth	850:859	the growth of MG-63 human osteoblast cells	850:891	Further, we investigated the effect of the composite coatings on the growth of MG-63 human osteoblast cells to assess their biocompatibility.
28732850	3	19	theme	chitosan	466:473	arg1	inclusion					475:483	chitosan inclusion	466:483	chitosan inclusion	466:483	Surface characterization results revealed the influence of chitosan inclusion on polypyrrole (PPy) surface morphology.
28732850	3	20	theme	Surface	407:413	arg1	results					432:438	Surface characterization results	407:438	Surface characterization results	407:438	Surface characterization results revealed the influence of chitosan inclusion on polypyrrole (PPy) surface morphology.
28732850	3	21	dep	polypyrrole	488:498	arg1	PPy					501:503	PPy	501:503	PPy	501:503	Surface characterization results revealed the influence of chitosan inclusion on polypyrrole (PPy) surface morphology.
28732850	5	22	theme	Electrochemical	653:667	arg1	studies					679:685	Electrochemical corrosion studies	653:685	Electrochemical corrosion studies	653:685	Electrochemical corrosion studies revealed that the composite coatings showed enhanced protective performance compared to pure PPy.
28732850	0	23	theme	Promising	0:8	arg1	chitosan					44:51	chitosan	44:51	chitosan	44:51	Promising bio-composites of polypyrrole and chitosan: Surface protective and in vitro biocompatibility performance on 316L SS implants.
28732850	0	23	theme	Promising	0:8	arg1	polypyrrole					28:38	polypyrrole	28:38	polypyrrole	28:38	Promising bio-composites of polypyrrole and chitosan: Surface protective and in vitro biocompatibility performance on 316L SS implants.
28732850	0	23	theme	Promising	0:8	arg1	bio-composites					10:23	Promising bio-composites	0:23	Promising bio-composites of polypyrrole and chitosan: Surface protective and in vitro biocompatibility performance on 316L SS implants.	0:134	Promising bio-composites of polypyrrole and chitosan: Surface protective and in vitro biocompatibility performance on 316L SS implants.
28732850	5	24	theme	protective	740:749	arg1	performance					751:761	enhanced protective performance	731:761	enhanced protective performance	731:761	Electrochemical corrosion studies revealed that the composite coatings showed enhanced protective performance compared to pure PPy.
28732850	5	25	theme	corrosion	669:677	arg1	studies					679:685	Electrochemical corrosion studies	653:685	Electrochemical corrosion studies	653:685	Electrochemical corrosion studies revealed that the composite coatings showed enhanced protective performance compared to pure PPy.
28732850	0	26	dep	in	77:78	arg1	vitro					80:84	vitro	80:84	vitro	80:84	Promising bio-composites of polypyrrole and chitosan: Surface protective and in vitro biocompatibility performance on 316L SS implants.
28732850	7	27	theme	Carlo	933:937	arg1	simulations					939:949	Monte Carlo simulations	927:949	Monte Carlo simulations	927:949	Monte Carlo simulations were engaged to assess the interactions between the metal surface and composite coatings.
28732850	0	28	theme	polypyrrole	28:38	arg1	chitosan					44:51	chitosan	44:51	chitosan	44:51	Promising bio-composites of polypyrrole and chitosan: Surface protective and in vitro biocompatibility performance on 316L SS implants.
28732850	0	28	theme	polypyrrole	28:38	arg1	polypyrrole					28:38	polypyrrole	28:38	polypyrrole	28:38	Promising bio-composites of polypyrrole and chitosan: Surface protective and in vitro biocompatibility performance on 316L SS implants.
28732850	0	28	theme	polypyrrole	28:38	arg1	bio-composites					10:23	Promising bio-composites	0:23	Promising bio-composites of polypyrrole and chitosan: Surface protective and in vitro biocompatibility performance on 316L SS implants.	0:134	Promising bio-composites of polypyrrole and chitosan: Surface protective and in vitro biocompatibility performance on 316L SS implants.
28732850	6	29	theme	human	870:874	arg1	cells					887:891	MG-63 human osteoblast cells	864:891	MG-63 human osteoblast cells	864:891	Further, we investigated the effect of the composite coatings on the growth of MG-63 human osteoblast cells to assess their biocompatibility.
28732850	6	30	theme	MG-63	864:868	arg1	cells					887:891	MG-63 human osteoblast cells	864:891	MG-63 human osteoblast cells	864:891	Further, we investigated the effect of the composite coatings on the growth of MG-63 human osteoblast cells to assess their biocompatibility.
28732850	2	31	theme	electrochemical	353:367	arg1	polymerization					369:382	in-situ electrochemical polymerization	345:382	in-situ electrochemical polymerization in oxalic acid medium	345:404	We synthesized bioactive composites based on polypyrrole/chitosan through in-situ electrochemical polymerization in oxalic acid medium.
28732850	8	32	theme	containing	1055:1064	arg1	biocompatible					1111:1123	biocompatible	1111:1123	biocompatible	1111:1123	The composite containing equal parts PPy and chitosan was found to be biocompatible; together with the corrosion protection results, the findings indicated that this bioactive coating material has potential for use in 316L SS implants.
28732850	8	32	theme	containing	1055:1064	arg1	parts					1072:1076	The composite containing equal parts	1041:1076	The composite containing equal parts PPy and chitosan	1041:1093	The composite containing equal parts PPy and chitosan was found to be biocompatible; together with the corrosion protection results, the findings indicated that this bioactive coating material has potential for use in 316L SS implants.
28732850	8	32	theme	containing	1055:1064	arg1	chitosan					1086:1093	chitosan	1086:1093	chitosan	1086:1093	The composite containing equal parts PPy and chitosan was found to be biocompatible; together with the corrosion protection results, the findings indicated that this bioactive coating material has potential for use in 316L SS implants.
28732850	8	32	theme	containing	1055:1064	arg1	PPy					1078:1080	PPy	1078:1080	PPy	1078:1080	The composite containing equal parts PPy and chitosan was found to be biocompatible; together with the corrosion protection results, the findings indicated that this bioactive coating material has potential for use in 316L SS implants.
28732850	0	33	theme	SS	123:124	arg1	implants					126:133	316L SS implants	118:133	316L SS implants	118:133	Promising bio-composites of polypyrrole and chitosan: Surface protective and in vitro biocompatibility performance on 316L SS implants.
28732850	4	34	theme	Contact	526:532	arg1	results					540:546	Contact angle results	526:546	Contact angle results	526:546	Contact angle results confirmed the enhancement in surface hydrophilicity due to the addition of chitosan into the PPy matrix.
28732850	2	35	theme	in-situ	345:351	arg1	polymerization					369:382	in-situ electrochemical polymerization	345:382	in-situ electrochemical polymerization in oxalic acid medium	345:404	We synthesized bioactive composites based on polypyrrole/chitosan through in-situ electrochemical polymerization in oxalic acid medium.
28732850	1	36	theme	natural	216:222	arg1	polymers					238:245	natural biodegradable polymers	216:245	natural biodegradable polymers	216:245	Advanced biomedical materials can potentially be developed from combinations of natural biodegradable polymers and synthetic polymers.
28732850	0	37	theme	chitosan	44:51	arg1	chitosan					44:51	chitosan	44:51	chitosan	44:51	Promising bio-composites of polypyrrole and chitosan: Surface protective and in vitro biocompatibility performance on 316L SS implants.
28732850	0	37	theme	chitosan	44:51	arg1	polypyrrole					28:38	polypyrrole	28:38	polypyrrole	28:38	Promising bio-composites of polypyrrole and chitosan: Surface protective and in vitro biocompatibility performance on 316L SS implants.
28732850	0	37	theme	chitosan	44:51	arg1	bio-composites					10:23	Promising bio-composites	0:23	Promising bio-composites of polypyrrole and chitosan: Surface protective and in vitro biocompatibility performance on 316L SS implants.	0:134	Promising bio-composites of polypyrrole and chitosan: Surface protective and in vitro biocompatibility performance on 316L SS implants.
28732850	4	38	theme	PPy	641:643	arg1	matrix					645:650	the PPy matrix	637:650	the PPy matrix	637:650	Contact angle results confirmed the enhancement in surface hydrophilicity due to the addition of chitosan into the PPy matrix.
28732850	8	39	from	use	1252:1254	arg1	implants					1267:1274	316L SS implants	1259:1274	316L SS implants	1259:1274	The composite containing equal parts PPy and chitosan was found to be biocompatible; together with the corrosion protection results, the findings indicated that this bioactive coating material has potential for use in 316L SS implants.
28732850	7	40	theme	Monte	927:931	arg1	simulations					939:949	Monte Carlo simulations	927:949	Monte Carlo simulations	927:949	Monte Carlo simulations were engaged to assess the interactions between the metal surface and composite coatings.
28732850	1	41	theme	biodegradable	224:236	arg1	polymers					238:245	natural biodegradable polymers	216:245	natural biodegradable polymers	216:245	Advanced biomedical materials can potentially be developed from combinations of natural biodegradable polymers and synthetic polymers.
28732850	8	42	theme	protection	1154:1163	arg1	results					1165:1171	the corrosion protection results	1140:1171	the corrosion protection results	1140:1171	The composite containing equal parts PPy and chitosan was found to be biocompatible; together with the corrosion protection results, the findings indicated that this bioactive coating material has potential for use in 316L SS implants.
28732850	1	43	theme	polymers	238:245	arg1	combinations					200:211	combinations	200:211	combinations of natural biodegradable polymers and synthetic polymers	200:268	Advanced biomedical materials can potentially be developed from combinations of natural biodegradable polymers and synthetic polymers.
28732850	3	44	theme	inclusion	475:483	arg1	influence					453:461	the influence	449:461	the influence of chitosan inclusion on polypyrrole (PPy) surface morphology	449:523	Surface characterization results revealed the influence of chitosan inclusion on polypyrrole (PPy) surface morphology.
28732850	8	45	theme	coating	1217:1223	arg1	material					1225:1232	this bioactive coating material	1202:1232	this bioactive coating material	1202:1232	The composite containing equal parts PPy and chitosan was found to be biocompatible; together with the corrosion protection results, the findings indicated that this bioactive coating material has potential for use in 316L SS implants.
28732850	4	46	theme	angle	534:538	arg1	results					540:546	Contact angle results	526:546	Contact angle results	526:546	Contact angle results confirmed the enhancement in surface hydrophilicity due to the addition of chitosan into the PPy matrix.
28732850	4	47	theme	chitosan	623:630	arg1	addition					611:618	the addition	607:618	the addition of chitosan into the PPy matrix	607:650	Contact angle results confirmed the enhancement in surface hydrophilicity due to the addition of chitosan into the PPy matrix.
28732850	8	48	theme	bioactive	1207:1215	arg1	material					1225:1232	this bioactive coating material	1202:1232	this bioactive coating material	1202:1232	The composite containing equal parts PPy and chitosan was found to be biocompatible; together with the corrosion protection results, the findings indicated that this bioactive coating material has potential for use in 316L SS implants.
28732850	4	49	from	enhancement	562:572	arg1	hydrophilicity					585:598	surface hydrophilicity	577:598	surface hydrophilicity due to the addition of chitosan into the PPy matrix	577:650	Contact angle results confirmed the enhancement in surface hydrophilicity due to the addition of chitosan into the PPy matrix.
28732850	8	50	theme	composite	1045:1053	arg1	biocompatible					1111:1123	biocompatible	1111:1123	biocompatible	1111:1123	The composite containing equal parts PPy and chitosan was found to be biocompatible; together with the corrosion protection results, the findings indicated that this bioactive coating material has potential for use in 316L SS implants.
28732850	8	50	theme	composite	1045:1053	arg1	parts					1072:1076	The composite containing equal parts	1041:1076	The composite containing equal parts PPy and chitosan	1041:1093	The composite containing equal parts PPy and chitosan was found to be biocompatible; together with the corrosion protection results, the findings indicated that this bioactive coating material has potential for use in 316L SS implants.
28732850	8	50	theme	composite	1045:1053	arg1	chitosan					1086:1093	chitosan	1086:1093	chitosan	1086:1093	The composite containing equal parts PPy and chitosan was found to be biocompatible; together with the corrosion protection results, the findings indicated that this bioactive coating material has potential for use in 316L SS implants.
28732850	8	50	theme	composite	1045:1053	arg1	PPy					1078:1080	PPy	1078:1080	PPy	1078:1080	The composite containing equal parts PPy and chitosan was found to be biocompatible; together with the corrosion protection results, the findings indicated that this bioactive coating material has potential for use in 316L SS implants.
28732850	8	51	contain	has	1234:1236	arg2	potential					1238:1246	potential	1238:1246	potential for use in 316L SS implants	1238:1274	The composite containing equal parts PPy and chitosan was found to be biocompatible; together with the corrosion protection results, the findings indicated that this bioactive coating material has potential for use in 316L SS implants.
28732850	8	51	contain	has	1234:1236	arg1	material					1225:1232	this bioactive coating material	1202:1232	this bioactive coating material	1202:1232	The composite containing equal parts PPy and chitosan was found to be biocompatible; together with the corrosion protection results, the findings indicated that this bioactive coating material has potential for use in 316L SS implants.
28732850	6	52	theme	osteoblast	876:885	arg1	cells					887:891	MG-63 human osteoblast cells	864:891	MG-63 human osteoblast cells	864:891	Further, we investigated the effect of the composite coatings on the growth of MG-63 human osteoblast cells to assess their biocompatibility.
28732850	2	53	theme	acid	394:397	arg1	medium					399:404	oxalic acid medium	387:404	oxalic acid medium	387:404	We synthesized bioactive composites based on polypyrrole/chitosan through in-situ electrochemical polymerization in oxalic acid medium.
28732850	7	54	theme	composite	1021:1029	arg1	coatings					1031:1038	the metal surface and composite coatings	999:1038	coatings	1031:1038	Monte Carlo simulations were engaged to assess the interactions between the metal surface and composite coatings.
28732850	2	55	theme	bioactive	286:294	arg1	composites					296:305	bioactive composites	286:305	bioactive composites based on polypyrrole/chitosan	286:335	We synthesized bioactive composites based on polypyrrole/chitosan through in-situ electrochemical polymerization in oxalic acid medium.
28732850	6	56	theme	coatings	838:845	arg1	effect					814:819	the effect	810:819	the effect of the composite coatings on the growth of MG-63 human osteoblast cells	810:891	Further, we investigated the effect of the composite coatings on the growth of MG-63 human osteoblast cells to assess their biocompatibility.
28732850	1	57	theme	Advanced	136:143	arg1	materials					156:164	Advanced biomedical materials	136:164	Advanced biomedical materials	136:164	Advanced biomedical materials can potentially be developed from combinations of natural biodegradable polymers and synthetic polymers.
28732850	7	58	theme	metal	1003:1007	arg1	surface					1009:1015	the metal surface and composite coatings	999:1038	surface	1009:1015	Monte Carlo simulations were engaged to assess the interactions between the metal surface and composite coatings.
28732850	1	59	theme	synthetic	251:259	arg1	polymers					261:268	synthetic polymers	251:268	synthetic polymers	251:268	Advanced biomedical materials can potentially be developed from combinations of natural biodegradable polymers and synthetic polymers.
28732850	0	60	dep	bio-composites	10:23	arg1	performance					103:113	in vitro biocompatibility performance	77:113	in vitro biocompatibility performance	77:113	Promising bio-composites of polypyrrole and chitosan: Surface protective and in vitro biocompatibility performance on 316L SS implants.
28732850	0	60	dep	bio-composites	10:23	arg1	protective					62:71	protective	62:71	protective	62:71	Promising bio-composites of polypyrrole and chitosan: Surface protective and in vitro biocompatibility performance on 316L SS implants.
28732850	6	61	theme	composite	828:836	arg1	coatings					838:845	the composite coatings	824:845	the composite coatings	824:845	Further, we investigated the effect of the composite coatings on the growth of MG-63 human osteoblast cells to assess their biocompatibility.
28732850	1	62	theme	biomedical	145:154	arg1	materials					156:164	Advanced biomedical materials	136:164	Advanced biomedical materials	136:164	Advanced biomedical materials can potentially be developed from combinations of natural biodegradable polymers and synthetic polymers.
28732850	8	63	dep	parts	1072:1076	arg1	parts					1072:1076	The composite containing equal parts	1041:1076	The composite containing equal parts PPy and chitosan	1041:1093	The composite containing equal parts PPy and chitosan was found to be biocompatible; together with the corrosion protection results, the findings indicated that this bioactive coating material has potential for use in 316L SS implants.
28732850	8	63	dep	parts	1072:1076	arg1	chitosan					1086:1093	chitosan	1086:1093	chitosan	1086:1093	The composite containing equal parts PPy and chitosan was found to be biocompatible; together with the corrosion protection results, the findings indicated that this bioactive coating material has potential for use in 316L SS implants.
28732850	8	63	dep	parts	1072:1076	arg1	PPy					1078:1080	PPy	1078:1080	PPy	1078:1080	The composite containing equal parts PPy and chitosan was found to be biocompatible; together with the corrosion protection results, the findings indicated that this bioactive coating material has potential for use in 316L SS implants.
28732850	1	64	theme	polymers	261:268	arg1	combinations					200:211	combinations	200:211	combinations of natural biodegradable polymers and synthetic polymers	200:268	Advanced biomedical materials can potentially be developed from combinations of natural biodegradable polymers and synthetic polymers.
28732850	8	65	theme	316L	1259:1262	arg1	implants					1267:1274	316L SS implants	1259:1274	316L SS implants	1259:1274	The composite containing equal parts PPy and chitosan was found to be biocompatible; together with the corrosion protection results, the findings indicated that this bioactive coating material has potential for use in 316L SS implants.
27981048	8	0	theme	root	1269:1272	arg1	sealer					1280:1285	root canal sealer	1269:1285	root canal sealer	1269:1285	When low-viscosity MC or HPC was added, the composition of CSCs changed, thus fulfilling the requirements for use as root canal sealer.
27981048	4	1	theme	higher	750:755	arg1	values					762:767	significantly higher flow values	736:767	significantly higher flow values	736:767	MC and HPC groups exhibited significantly higher flow values and setting times than other groups (p < 0.05).
27981048	3	2	theme	2 wt	640:643	arg1	MC					660:661	2 wt% low-viscosity MC	640:661	2 wt% low-viscosity MC (MC group)	640:672	MTA and low-heat Portland cement (LPC) including 20% fine particle zirconium oxide (ZO group), LPC including zirconium oxide and 2 wt% low-viscosity MC (MC group), and HPC (HPC group) were tested.
27981048	3	2	theme	2 wt	640:643	arg1	group					667:671	MC group	664:671	MC group	664:671	MTA and low-heat Portland cement (LPC) including 20% fine particle zirconium oxide (ZO group), LPC including zirconium oxide and 2 wt% low-viscosity MC (MC group), and HPC (HPC group) were tested.
27981048	2	3	theme	different	308:316	arg1	compositions					318:329	different compositions	308:329	different compositions containing low-viscosity methyl cellulose (MC) or hydroxypropyl cellulose (HPC) additive	308:418	The flow, working time, and setting time of CSCs with different compositions containing low-viscosity methyl cellulose (MC) or hydroxypropyl cellulose (HPC) additive were examined according to ISO 6876-2012; calcium ion release analysis was also conducted.
27981048	2	4	with	time	272:275	arg1	compositions					318:329	different compositions	308:329	different compositions containing low-viscosity methyl cellulose (MC) or hydroxypropyl cellulose (HPC) additive	308:418	The flow, working time, and setting time of CSCs with different compositions containing low-viscosity methyl cellulose (MC) or hydroxypropyl cellulose (HPC) additive were examined according to ISO 6876-2012; calcium ion release analysis was also conducted.
27981048	0	5	theme	Hydroxypropyl	79:91	arg1	Cellulose					93:101	Hydroxypropyl Cellulose Additive	79:110	Hydroxypropyl Cellulose Additive	79:110	Examination of Calcium Silicate Cements with Low-Viscosity Methyl Cellulose or Hydroxypropyl Cellulose Additive.
27981048	7	6	theme	ions	1066:1069	arg1	concentration					1041:1053	The concentration	1037:1053	The concentration of calcium ions	1037:1069	The concentration of calcium ions was decreased by the addition of the MC or HPC group compared with the ZO group.
27981048	6	7	theme	HPC	1007:1009	arg1	groups					1011:1016	HPC groups	1007:1016	HPC groups	1007:1016	Calcium ion release was retarded with ZO, MC, and HPC groups compared with MTA.
27981048	8	8	theme	CSCs	1211:1214	arg1	composition					1196:1206	the composition	1192:1206	the composition of CSCs	1192:1214	When low-viscosity MC or HPC was added, the composition of CSCs changed, thus fulfilling the requirements for use as root canal sealer.
27981048	2	9	theme	release	474:480	arg1	analysis					482:489	calcium ion release analysis	462:489	calcium ion release analysis	462:489	The flow, working time, and setting time of CSCs with different compositions containing low-viscosity methyl cellulose (MC) or hydroxypropyl cellulose (HPC) additive were examined according to ISO 6876-2012; calcium ion release analysis was also conducted.
27981048	3	10	theme	%	644:644	arg1	MC					660:661	2 wt% low-viscosity MC	640:661	2 wt% low-viscosity MC (MC group)	640:672	MTA and low-heat Portland cement (LPC) including 20% fine particle zirconium oxide (ZO group), LPC including zirconium oxide and 2 wt% low-viscosity MC (MC group), and HPC (HPC group) were tested.
27981048	3	10	theme	%	644:644	arg1	group					667:671	MC group	664:671	MC group	664:671	MTA and low-heat Portland cement (LPC) including 20% fine particle zirconium oxide (ZO group), LPC including zirconium oxide and 2 wt% low-viscosity MC (MC group), and HPC (HPC group) were tested.
27981048	4	11	theme	flow	757:760	arg1	values					762:767	significantly higher flow values	736:767	significantly higher flow values	736:767	MC and HPC groups exhibited significantly higher flow values and setting times than other groups (p < 0.05).
27981048	7	12	theme	calcium	1058:1064	arg1	ions					1066:1069	calcium ions	1058:1069	calcium ions	1058:1069	The concentration of calcium ions was decreased by the addition of the MC or HPC group compared with the ZO group.
27981048	1	13	theme	cement	246:251	arg1	trioxide					221:228	mineral trioxide	213:228	mineral trioxide aggregate (MTA) cement	213:251	The purpose of this study was to improve the operability of calcium silicate cements (CSCs) such as mineral trioxide aggregate (MTA) cement.
27981048	2	14	theme	ion	470:472	arg1	release					474:480	calcium ion release	462:480	calcium ion release analysis	462:489	The flow, working time, and setting time of CSCs with different compositions containing low-viscosity methyl cellulose (MC) or hydroxypropyl cellulose (HPC) additive were examined according to ISO 6876-2012; calcium ion release analysis was also conducted.
27981048	3	15	theme	low-viscosity	646:658	arg1	MC					660:661	2 wt% low-viscosity MC	640:661	2 wt% low-viscosity MC (MC group)	640:672	MTA and low-heat Portland cement (LPC) including 20% fine particle zirconium oxide (ZO group), LPC including zirconium oxide and 2 wt% low-viscosity MC (MC group), and HPC (HPC group) were tested.
27981048	3	15	theme	low-viscosity	646:658	arg1	group					667:671	MC group	664:671	MC group	664:671	MTA and low-heat Portland cement (LPC) including 20% fine particle zirconium oxide (ZO group), LPC including zirconium oxide and 2 wt% low-viscosity MC (MC group), and HPC (HPC group) were tested.
27981048	4	16	theme	HPC	715:717	arg1	groups					719:724	MC and HPC groups	708:724	MC and HPC groups	708:724	MC and HPC groups exhibited significantly higher flow values and setting times than other groups (p < 0.05).
27981048	5	17	theme	flow	831:834	arg1	values					836:841	flow values	831:841	flow values of these groups	831:857	Additionally, flow values of these groups were higher than the ISO 6876-2012 reference values; furthermore, working times were over 10 min.
27981048	1	18	theme	silicate	181:188	arg1	operability					158:168	the operability	154:168	the operability of calcium silicate cements (CSCs) such as mineral trioxide aggregate (MTA) cement	154:251	The purpose of this study was to improve the operability of calcium silicate cements (CSCs) such as mineral trioxide aggregate (MTA) cement.
27981048	2	19	theme	calcium	462:468	arg1	release					474:480	calcium ion release	462:480	calcium ion release analysis	462:489	The flow, working time, and setting time of CSCs with different compositions containing low-viscosity methyl cellulose (MC) or hydroxypropyl cellulose (HPC) additive were examined according to ISO 6876-2012; calcium ion release analysis was also conducted.
27981048	6	20	theme	ion	965:967	arg1	release					969:975	Calcium ion release	957:975	Calcium ion release	957:975	Calcium ion release was retarded with ZO, MC, and HPC groups compared with MTA.
27981048	1	21	theme	calcium	173:179	arg1	silicate					181:188	calcium silicate cements	173:196	calcium silicate cements (CSCs)	173:203	The purpose of this study was to improve the operability of calcium silicate cements (CSCs) such as mineral trioxide aggregate (MTA) cement.
27981048	1	21	theme	calcium	173:179	arg1	CSCs					199:202	CSCs	199:202	CSCs	199:202	The purpose of this study was to improve the operability of calcium silicate cements (CSCs) such as mineral trioxide aggregate (MTA) cement.
27981048	2	22	contain	containing	331:340	arg2	hydroxypropyl					381:393	hydroxypropyl	381:393	hydroxypropyl	381:393	The flow, working time, and setting time of CSCs with different compositions containing low-viscosity methyl cellulose (MC) or hydroxypropyl cellulose (HPC) additive were examined according to ISO 6876-2012; calcium ion release analysis was also conducted.
27981048	2	22	contain	containing	331:340	arg1	compositions					318:329	different compositions	308:329	different compositions containing low-viscosity methyl cellulose (MC) or hydroxypropyl cellulose (HPC) additive	308:418	The flow, working time, and setting time of CSCs with different compositions containing low-viscosity methyl cellulose (MC) or hydroxypropyl cellulose (HPC) additive were examined according to ISO 6876-2012; calcium ion release analysis was also conducted.
27981048	2	22	contain	containing	331:340	arg2	MC					374:375	MC	374:375	MC	374:375	The flow, working time, and setting time of CSCs with different compositions containing low-viscosity methyl cellulose (MC) or hydroxypropyl cellulose (HPC) additive were examined according to ISO 6876-2012; calcium ion release analysis was also conducted.
27981048	2	22	contain	containing	331:340	arg2	cellulose					363:371	low-viscosity methyl cellulose	342:371	low-viscosity methyl cellulose (MC)	342:376	The flow, working time, and setting time of CSCs with different compositions containing low-viscosity methyl cellulose (MC) or hydroxypropyl cellulose (HPC) additive were examined according to ISO 6876-2012; calcium ion release analysis was also conducted.
27981048	3	23	theme	low-heat	519:526	arg1	LPC					545:547	LPC	545:547	LPC	545:547	MTA and low-heat Portland cement (LPC) including 20% fine particle zirconium oxide (ZO group), LPC including zirconium oxide and 2 wt% low-viscosity MC (MC group), and HPC (HPC group) were tested.
27981048	3	23	theme	low-heat	519:526	arg1	cement					537:542	low-heat Portland cement	519:542	low-heat Portland cement (LPC)	519:548	MTA and low-heat Portland cement (LPC) including 20% fine particle zirconium oxide (ZO group), LPC including zirconium oxide and 2 wt% low-viscosity MC (MC group), and HPC (HPC group) were tested.
27981048	2	24	theme	methyl	356:361	arg1	cellulose					363:371	low-viscosity methyl cellulose	342:371	low-viscosity methyl cellulose (MC)	342:376	The flow, working time, and setting time of CSCs with different compositions containing low-viscosity methyl cellulose (MC) or hydroxypropyl cellulose (HPC) additive were examined according to ISO 6876-2012; calcium ion release analysis was also conducted.
27981048	2	24	theme	methyl	356:361	arg1	MC					374:375	MC	374:375	MC	374:375	The flow, working time, and setting time of CSCs with different compositions containing low-viscosity methyl cellulose (MC) or hydroxypropyl cellulose (HPC) additive were examined according to ISO 6876-2012; calcium ion release analysis was also conducted.
27981048	3	25	theme	MC	664:665	arg1	MC					660:661	2 wt% low-viscosity MC	640:661	2 wt% low-viscosity MC (MC group)	640:672	MTA and low-heat Portland cement (LPC) including 20% fine particle zirconium oxide (ZO group), LPC including zirconium oxide and 2 wt% low-viscosity MC (MC group), and HPC (HPC group) were tested.
27981048	3	25	theme	MC	664:665	arg1	group					667:671	MC group	664:671	MC group	664:671	MTA and low-heat Portland cement (LPC) including 20% fine particle zirconium oxide (ZO group), LPC including zirconium oxide and 2 wt% low-viscosity MC (MC group), and HPC (HPC group) were tested.
27981048	9	26	theme	CSC	1345:1347	arg1	composition					1349:1359	the CSC composition	1341:1359	the CSC composition	1341:1359	Calcium ion release by CSCs was affected by changing the CSC composition via the addition of MC or HPC.
27981048	3	27	theme	Portland	528:535	arg1	LPC					545:547	LPC	545:547	LPC	545:547	MTA and low-heat Portland cement (LPC) including 20% fine particle zirconium oxide (ZO group), LPC including zirconium oxide and 2 wt% low-viscosity MC (MC group), and HPC (HPC group) were tested.
27981048	3	27	theme	Portland	528:535	arg1	cement					537:542	low-heat Portland cement	519:542	low-heat Portland cement (LPC)	519:548	MTA and low-heat Portland cement (LPC) including 20% fine particle zirconium oxide (ZO group), LPC including zirconium oxide and 2 wt% low-viscosity MC (MC group), and HPC (HPC group) were tested.
27981048	2	28	theme	low-viscosity	342:354	arg1	cellulose					363:371	low-viscosity methyl cellulose	342:371	low-viscosity methyl cellulose (MC)	342:376	The flow, working time, and setting time of CSCs with different compositions containing low-viscosity methyl cellulose (MC) or hydroxypropyl cellulose (HPC) additive were examined according to ISO 6876-2012; calcium ion release analysis was also conducted.
27981048	2	28	theme	low-viscosity	342:354	arg1	MC					374:375	MC	374:375	MC	374:375	The flow, working time, and setting time of CSCs with different compositions containing low-viscosity methyl cellulose (MC) or hydroxypropyl cellulose (HPC) additive were examined according to ISO 6876-2012; calcium ion release analysis was also conducted.
27981048	0	29	theme	Silicate	23:30	arg1	Examination					0:10	Examination	0:10	Examination of Calcium Silicate	0:30	Examination of Calcium Silicate Cements with Low-Viscosity Methyl Cellulose or Hydroxypropyl Cellulose Additive.
27981048	7	30	theme	ZO	1142:1143	arg1	group					1145:1149	the ZO group	1138:1149	the ZO group	1138:1149	The concentration of calcium ions was decreased by the addition of the MC or HPC group compared with the ZO group.
27981048	5	31	theme	6876-2012	884:892	arg1	values					904:909	the ISO 6876-2012 reference values	876:909	the ISO 6876-2012 reference values	876:909	Additionally, flow values of these groups were higher than the ISO 6876-2012 reference values; furthermore, working times were over 10 min.
27981048	9	32	theme	MC	1381:1382	arg1	addition					1369:1376	the addition	1365:1376	the addition of MC or HPC	1365:1389	Calcium ion release by CSCs was affected by changing the CSC composition via the addition of MC or HPC.
27981048	0	33	theme	Calcium	15:21	arg1	Silicate					23:30	Calcium Silicate	15:30	Calcium Silicate	15:30	Examination of Calcium Silicate Cements with Low-Viscosity Methyl Cellulose or Hydroxypropyl Cellulose Additive.
27981048	4	34	theme	other	792:796	arg1	groups					798:803	other groups	792:803	other groups (p < 0.05)	792:814	MC and HPC groups exhibited significantly higher flow values and setting times than other groups (p < 0.05).
27981048	4	34	theme	other	792:796	arg1	<					808:808	p < 0.05	806:813	p < 0.05	806:813	MC and HPC groups exhibited significantly higher flow values and setting times than other groups (p < 0.05).
27981048	2	35	with	time	290:293	arg1	compositions					318:329	different compositions	308:329	different compositions containing low-viscosity methyl cellulose (MC) or hydroxypropyl cellulose (HPC) additive	308:418	The flow, working time, and setting time of CSCs with different compositions containing low-viscosity methyl cellulose (MC) or hydroxypropyl cellulose (HPC) additive were examined according to ISO 6876-2012; calcium ion release analysis was also conducted.
27981048	2	36	theme	working	264:270	arg1	time					272:275	working time	264:275	working time	264:275	The flow, working time, and setting time of CSCs with different compositions containing low-viscosity methyl cellulose (MC) or hydroxypropyl cellulose (HPC) additive were examined according to ISO 6876-2012; calcium ion release analysis was also conducted.
27981048	7	37	theme	HPC	1114:1116	arg1	group					1118:1122	the MC or HPC group	1104:1122	group	1118:1122	The concentration of calcium ions was decreased by the addition of the MC or HPC group compared with the ZO group.
27981048	4	38	theme	p	806:806	arg1	groups					798:803	other groups	792:803	other groups (p < 0.05)	792:814	MC and HPC groups exhibited significantly higher flow values and setting times than other groups (p < 0.05).
27981048	4	38	theme	p	806:806	arg1	<					808:808	p < 0.05	806:813	p < 0.05	806:813	MC and HPC groups exhibited significantly higher flow values and setting times than other groups (p < 0.05).
27981048	1	39	dep	silicate	181:188	arg1	cements					190:196	cements	190:196	cements	190:196	The purpose of this study was to improve the operability of calcium silicate cements (CSCs) such as mineral trioxide aggregate (MTA) cement.
27981048	9	40	theme	HPC	1387:1389	arg1	addition					1369:1376	the addition	1365:1376	the addition of MC or HPC	1365:1389	Calcium ion release by CSCs was affected by changing the CSC composition via the addition of MC or HPC.
27981048	3	41	theme	particle	569:576	arg1	oxide					588:592	20% fine particle zirconium oxide	560:592	20% fine particle zirconium oxide (ZO group)	560:603	MTA and low-heat Portland cement (LPC) including 20% fine particle zirconium oxide (ZO group), LPC including zirconium oxide and 2 wt% low-viscosity MC (MC group), and HPC (HPC group) were tested.
27981048	3	41	theme	particle	569:576	arg1	group					598:602	ZO group	595:602	ZO group	595:602	MTA and low-heat Portland cement (LPC) including 20% fine particle zirconium oxide (ZO group), LPC including zirconium oxide and 2 wt% low-viscosity MC (MC group), and HPC (HPC group) were tested.
27981048	3	42	theme	HPC	684:686	arg1	group					688:692	HPC group	684:692	HPC group	684:692	MTA and low-heat Portland cement (LPC) including 20% fine particle zirconium oxide (ZO group), LPC including zirconium oxide and 2 wt% low-viscosity MC (MC group), and HPC (HPC group) were tested.
27981048	3	42	theme	HPC	684:686	arg1	HPC					679:681	HPC	679:681	HPC (HPC group)	679:693	MTA and low-heat Portland cement (LPC) including 20% fine particle zirconium oxide (ZO group), LPC including zirconium oxide and 2 wt% low-viscosity MC (MC group), and HPC (HPC group) were tested.
27981048	0	43	theme	Methyl	59:64	arg1	Cellulose					66:74	Low-Viscosity Methyl Cellulose	45:74	Low-Viscosity Methyl Cellulose	45:74	Examination of Calcium Silicate Cements with Low-Viscosity Methyl Cellulose or Hydroxypropyl Cellulose Additive.
27981048	4	44	theme	setting	773:779	arg1	times					781:785	setting times	773:785	setting times	773:785	MC and HPC groups exhibited significantly higher flow values and setting times than other groups (p < 0.05).
27981048	3	45	theme	zirconium	578:586	arg1	oxide					588:592	20% fine particle zirconium oxide	560:592	20% fine particle zirconium oxide (ZO group)	560:603	MTA and low-heat Portland cement (LPC) including 20% fine particle zirconium oxide (ZO group), LPC including zirconium oxide and 2 wt% low-viscosity MC (MC group), and HPC (HPC group) were tested.
27981048	3	45	theme	zirconium	578:586	arg1	group					598:602	ZO group	595:602	ZO group	595:602	MTA and low-heat Portland cement (LPC) including 20% fine particle zirconium oxide (ZO group), LPC including zirconium oxide and 2 wt% low-viscosity MC (MC group), and HPC (HPC group) were tested.
27981048	4	46	theme	MC	708:709	arg1	groups					719:724	MC and HPC groups	708:724	MC and HPC groups	708:724	MC and HPC groups exhibited significantly higher flow values and setting times than other groups (p < 0.05).
27981048	8	47	theme	low-viscosity	1157:1169	arg1	MC					1171:1172	low-viscosity MC	1157:1172	low-viscosity MC	1157:1172	When low-viscosity MC or HPC was added, the composition of CSCs changed, thus fulfilling the requirements for use as root canal sealer.
27981048	2	48	theme	CSCs	298:301	arg1	flow					258:261	The flow	254:261	The flow	254:261	The flow, working time, and setting time of CSCs with different compositions containing low-viscosity methyl cellulose (MC) or hydroxypropyl cellulose (HPC) additive were examined according to ISO 6876-2012; calcium ion release analysis was also conducted.
27981048	2	48	theme	CSCs	298:301	arg1	time					272:275	working time	264:275	working time	264:275	The flow, working time, and setting time of CSCs with different compositions containing low-viscosity methyl cellulose (MC) or hydroxypropyl cellulose (HPC) additive were examined according to ISO 6876-2012; calcium ion release analysis was also conducted.
27981048	2	48	theme	CSCs	298:301	arg1	time					290:293	setting time	282:293	setting time	282:293	The flow, working time, and setting time of CSCs with different compositions containing low-viscosity methyl cellulose (MC) or hydroxypropyl cellulose (HPC) additive were examined according to ISO 6876-2012; calcium ion release analysis was also conducted.
27981048	0	49	theme	Low-Viscosity	45:57	arg1	Cellulose					66:74	Low-Viscosity Methyl Cellulose	45:74	Low-Viscosity Methyl Cellulose	45:74	Examination of Calcium Silicate Cements with Low-Viscosity Methyl Cellulose or Hydroxypropyl Cellulose Additive.
27981048	8	50	theme	canal	1274:1278	arg1	sealer					1280:1285	root canal sealer	1269:1285	root canal sealer	1269:1285	When low-viscosity MC or HPC was added, the composition of CSCs changed, thus fulfilling the requirements for use as root canal sealer.
27981048	2	51	theme	additive	411:418	arg1	cellulose					363:371	low-viscosity methyl cellulose	342:371	low-viscosity methyl cellulose (MC)	342:376	The flow, working time, and setting time of CSCs with different compositions containing low-viscosity methyl cellulose (MC) or hydroxypropyl cellulose (HPC) additive were examined according to ISO 6876-2012; calcium ion release analysis was also conducted.
27981048	2	51	theme	additive	411:418	arg1	MC					374:375	MC	374:375	MC	374:375	The flow, working time, and setting time of CSCs with different compositions containing low-viscosity methyl cellulose (MC) or hydroxypropyl cellulose (HPC) additive were examined according to ISO 6876-2012; calcium ion release analysis was also conducted.
27981048	5	52	theme	ISO	880:882	arg1	values					904:909	the ISO 6876-2012 reference values	876:909	the ISO 6876-2012 reference values	876:909	Additionally, flow values of these groups were higher than the ISO 6876-2012 reference values; furthermore, working times were over 10 min.
27981048	5	53	theme	working	925:931	arg1	times					933:937	working times	925:937	working times	925:937	Additionally, flow values of these groups were higher than the ISO 6876-2012 reference values; furthermore, working times were over 10 min.
27981048	3	54	theme	20	560:561	arg1	%					562:562	%	562:562	%	562:562	MTA and low-heat Portland cement (LPC) including 20% fine particle zirconium oxide (ZO group), LPC including zirconium oxide and 2 wt% low-viscosity MC (MC group), and HPC (HPC group) were tested.
27981048	5	55	theme	reference	894:902	arg1	values					904:909	the ISO 6876-2012 reference values	876:909	the ISO 6876-2012 reference values	876:909	Additionally, flow values of these groups were higher than the ISO 6876-2012 reference values; furthermore, working times were over 10 min.
27981048	3	56	theme	%	562:562	arg1	oxide					588:592	20% fine particle zirconium oxide	560:592	20% fine particle zirconium oxide (ZO group)	560:603	MTA and low-heat Portland cement (LPC) including 20% fine particle zirconium oxide (ZO group), LPC including zirconium oxide and 2 wt% low-viscosity MC (MC group), and HPC (HPC group) were tested.
27981048	3	56	theme	%	562:562	arg1	group					598:602	ZO group	595:602	ZO group	595:602	MTA and low-heat Portland cement (LPC) including 20% fine particle zirconium oxide (ZO group), LPC including zirconium oxide and 2 wt% low-viscosity MC (MC group), and HPC (HPC group) were tested.
27981048	1	57	theme	study	133:137	arg1	purpose					117:123	The purpose	113:123	The purpose of this study	113:137	The purpose of this study was to improve the operability of calcium silicate cements (CSCs) such as mineral trioxide aggregate (MTA) cement.
27981048	6	58	theme	Calcium	957:963	arg1	release					969:975	Calcium ion release	957:975	Calcium ion release	957:975	Calcium ion release was retarded with ZO, MC, and HPC groups compared with MTA.
27981048	3	59	theme	ZO	595:596	arg1	oxide					588:592	20% fine particle zirconium oxide	560:592	20% fine particle zirconium oxide (ZO group)	560:603	MTA and low-heat Portland cement (LPC) including 20% fine particle zirconium oxide (ZO group), LPC including zirconium oxide and 2 wt% low-viscosity MC (MC group), and HPC (HPC group) were tested.
27981048	3	59	theme	ZO	595:596	arg1	group					598:602	ZO group	595:602	ZO group	595:602	MTA and low-heat Portland cement (LPC) including 20% fine particle zirconium oxide (ZO group), LPC including zirconium oxide and 2 wt% low-viscosity MC (MC group), and HPC (HPC group) were tested.
27981048	3	60	theme	fine	564:567	arg1	oxide					588:592	20% fine particle zirconium oxide	560:592	20% fine particle zirconium oxide (ZO group)	560:603	MTA and low-heat Portland cement (LPC) including 20% fine particle zirconium oxide (ZO group), LPC including zirconium oxide and 2 wt% low-viscosity MC (MC group), and HPC (HPC group) were tested.
27981048	3	60	theme	fine	564:567	arg1	group					598:602	ZO group	595:602	ZO group	595:602	MTA and low-heat Portland cement (LPC) including 20% fine particle zirconium oxide (ZO group), LPC including zirconium oxide and 2 wt% low-viscosity MC (MC group), and HPC (HPC group) were tested.
27981048	2	61	theme	setting	282:288	arg1	time					290:293	setting time	282:293	setting time	282:293	The flow, working time, and setting time of CSCs with different compositions containing low-viscosity methyl cellulose (MC) or hydroxypropyl cellulose (HPC) additive were examined according to ISO 6876-2012; calcium ion release analysis was also conducted.
27981048	7	62	theme	group	1118:1122	arg1	addition					1092:1099	the addition	1088:1099	the addition of the MC or HPC group	1088:1122	The concentration of calcium ions was decreased by the addition of the MC or HPC group compared with the ZO group.
27981048	1	63	theme	mineral	213:219	arg1	trioxide					221:228	mineral trioxide	213:228	mineral trioxide aggregate (MTA) cement	213:251	The purpose of this study was to improve the operability of calcium silicate cements (CSCs) such as mineral trioxide aggregate (MTA) cement.
27981048	7	64	theme	MC	1108:1109	arg1	group					1118:1122	the MC or HPC group	1104:1122	group	1118:1122	The concentration of calcium ions was decreased by the addition of the MC or HPC group compared with the ZO group.
27981048	5	65	theme	groups	852:857	arg1	values					836:841	flow values	831:841	flow values of these groups	831:857	Additionally, flow values of these groups were higher than the ISO 6876-2012 reference values; furthermore, working times were over 10 min.
27981048	9	66	theme	Calcium	1288:1294	arg1	release					1300:1306	Calcium ion release	1288:1306	Calcium ion release by CSCs	1288:1314	Calcium ion release by CSCs was affected by changing the CSC composition via the addition of MC or HPC.
27981048	3	67	theme	zirconium	620:628	arg1	oxide					630:634	zirconium oxide	620:634	zirconium oxide	620:634	MTA and low-heat Portland cement (LPC) including 20% fine particle zirconium oxide (ZO group), LPC including zirconium oxide and 2 wt% low-viscosity MC (MC group), and HPC (HPC group) were tested.
27981048	2	68	with	flow	258:261	arg1	compositions					318:329	different compositions	308:329	different compositions containing low-viscosity methyl cellulose (MC) or hydroxypropyl cellulose (HPC) additive	308:418	The flow, working time, and setting time of CSCs with different compositions containing low-viscosity methyl cellulose (MC) or hydroxypropyl cellulose (HPC) additive were examined according to ISO 6876-2012; calcium ion release analysis was also conducted.
27981048	9	69	theme	ion	1296:1298	arg1	release					1300:1306	Calcium ion release	1288:1306	Calcium ion release by CSCs	1288:1314	Calcium ion release by CSCs was affected by changing the CSC composition via the addition of MC or HPC.
27981048	0	70	theme	Additive	103:110	arg1	Cellulose					93:101	Hydroxypropyl Cellulose Additive	79:110	Hydroxypropyl Cellulose Additive	79:110	Examination of Calcium Silicate Cements with Low-Viscosity Methyl Cellulose or Hydroxypropyl Cellulose Additive.
26949055	0	0	theme	bioethanol	74:83	arg1	production					85:94	bioethanol production	74:94	bioethanol production	74:94	A novel sono-assisted acid pretreatment of chili post harvest residue for bioethanol production.
26949055	4	1	theme	electron	718:725	arg1	SEM					739:741	SEM	739:741	SEM	739:741	Changes in structural properties of the biomass were studied in relation to the pretreatment process using scanning electron microscopy (SEM), X-ray diffraction (XRD) and Fourier Transform Infrared (FTIR) analysis, and the changes in chemical composition was also monitored.
26949055	4	1	theme	electron	718:725	arg1	microscopy					727:736	scanning electron microscopy	709:736	scanning electron microscopy (SEM)	709:742	Changes in structural properties of the biomass were studied in relation to the pretreatment process using scanning electron microscopy (SEM), X-ray diffraction (XRD) and Fourier Transform Infrared (FTIR) analysis, and the changes in chemical composition was also monitored.
26949055	2	2	theme	pretreatment	348:359	arg1	efficiency					361:370	the pretreatment efficiency	344:370	the pretreatment efficiency	344:370	Operational parameters that affect the pretreatment efficiency were studied and optimized.
26949055	4	3	theme	scanning	709:716	arg1	SEM					739:741	SEM	739:741	SEM	739:741	Changes in structural properties of the biomass were studied in relation to the pretreatment process using scanning electron microscopy (SEM), X-ray diffraction (XRD) and Fourier Transform Infrared (FTIR) analysis, and the changes in chemical composition was also monitored.
26949055	4	3	theme	scanning	709:716	arg1	microscopy					727:736	scanning electron microscopy	709:736	scanning electron microscopy (SEM)	709:742	Changes in structural properties of the biomass were studied in relation to the pretreatment process using scanning electron microscopy (SEM), X-ray diffraction (XRD) and Fourier Transform Infrared (FTIR) analysis, and the changes in chemical composition was also monitored.
26949055	4	4	dep	Infrared	791:798	arg1	FTIR					801:804	FTIR	801:804	FTIR	801:804	Changes in structural properties of the biomass were studied in relation to the pretreatment process using scanning electron microscopy (SEM), X-ray diffraction (XRD) and Fourier Transform Infrared (FTIR) analysis, and the changes in chemical composition was also monitored.
26949055	3	5	theme	succinic	556:563	arg1	acid					565:568	succinic acid	556:568	succinic acid	556:568	Inhibitor analysis of the hydrolyzate revealed that major fermentation inhibitors like furfural, hydroxymethyl furfural and organic acids like citric acid, succinic acid and propionic acid were absent.
26949055	3	6	theme	Inhibitor	400:408	arg1	analysis					410:417	Inhibitor analysis	400:417	Inhibitor analysis of the hydrolyzate	400:436	Inhibitor analysis of the hydrolyzate revealed that major fermentation inhibitors like furfural, hydroxymethyl furfural and organic acids like citric acid, succinic acid and propionic acid were absent.
26949055	4	7	from	Changes	602:608	arg1	properties					624:633	structural properties	613:633	structural properties of the biomass	613:648	Changes in structural properties of the biomass were studied in relation to the pretreatment process using scanning electron microscopy (SEM), X-ray diffraction (XRD) and Fourier Transform Infrared (FTIR) analysis, and the changes in chemical composition was also monitored.
26949055	5	8	theme	sugars	961:966	arg1	0.465g/g					940:947	0.465g/g	940:947	0.465g/g of reducing sugars on enzymatic hydrolysis	940:990	The biomass pretreated with the optimized novel method yielded 0.465g/g of reducing sugars on enzymatic hydrolysis.
26949055	3	9	theme	organic	524:530	arg1	acids					532:536	organic acids	524:536	organic acids	524:536	Inhibitor analysis of the hydrolyzate revealed that major fermentation inhibitors like furfural, hydroxymethyl furfural and organic acids like citric acid, succinic acid and propionic acid were absent.
26949055	1	10	theme	study	126:130	arg1	objective					101:109	The objective	97:109	The objective of the present study	97:130	The objective of the present study was to develop a sono-assisted acid pretreatment strategy for the effective removal of lignin and hemicelluloses and to improve the sugar yield from chili post harvest residue.
26949055	3	11	theme	major	452:456	arg1	inhibitors					471:480	major fermentation inhibitors	452:480	major fermentation inhibitors like furfural, hydroxymethyl furfural and organic acids like citric acid, succinic acid and propionic acid	452:587	Inhibitor analysis of the hydrolyzate revealed that major fermentation inhibitors like furfural, hydroxymethyl furfural and organic acids like citric acid, succinic acid and propionic acid were absent.
26949055	3	12	theme	hydrolyzate	426:436	arg1	analysis					410:417	Inhibitor analysis	400:417	Inhibitor analysis of the hydrolyzate	400:436	Inhibitor analysis of the hydrolyzate revealed that major fermentation inhibitors like furfural, hydroxymethyl furfural and organic acids like citric acid, succinic acid and propionic acid were absent.
26949055	1	13	theme	sono-assisted	149:161	arg1	strategy					181:188	a sono-assisted acid pretreatment strategy	147:188	a sono-assisted acid pretreatment strategy for the effective removal of lignin and hemicelluloses	147:243	The objective of the present study was to develop a sono-assisted acid pretreatment strategy for the effective removal of lignin and hemicelluloses and to improve the sugar yield from chili post harvest residue.
26949055	4	14	from	changes	825:831	arg1	composition					845:855	chemical composition	836:855	chemical composition	836:855	Changes in structural properties of the biomass were studied in relation to the pretreatment process using scanning electron microscopy (SEM), X-ray diffraction (XRD) and Fourier Transform Infrared (FTIR) analysis, and the changes in chemical composition was also monitored.
26949055	1	15	from	chili	281:285	arg1	yield					270:274	the sugar yield	260:274	the sugar yield from chili post harvest residue	260:306	The objective of the present study was to develop a sono-assisted acid pretreatment strategy for the effective removal of lignin and hemicelluloses and to improve the sugar yield from chili post harvest residue.
26949055	4	16	theme	structural	613:622	arg1	properties					624:633	structural properties	613:633	structural properties of the biomass	613:648	Changes in structural properties of the biomass were studied in relation to the pretreatment process using scanning electron microscopy (SEM), X-ray diffraction (XRD) and Fourier Transform Infrared (FTIR) analysis, and the changes in chemical composition was also monitored.
26949055	1	17	theme	acid	163:166	arg1	strategy					181:188	a sono-assisted acid pretreatment strategy	147:188	a sono-assisted acid pretreatment strategy for the effective removal of lignin and hemicelluloses	147:243	The objective of the present study was to develop a sono-assisted acid pretreatment strategy for the effective removal of lignin and hemicelluloses and to improve the sugar yield from chili post harvest residue.
26949055	0	18	theme	novel	2:6	arg1	pretreatment					27:38	A novel sono-assisted acid pretreatment	0:38	A novel sono-assisted acid pretreatment of chili post harvest residue for bioethanol production.	0:95	A novel sono-assisted acid pretreatment of chili post harvest residue for bioethanol production.
26949055	4	19	theme	chemical	836:843	arg1	composition					845:855	chemical composition	836:855	chemical composition	836:855	Changes in structural properties of the biomass were studied in relation to the pretreatment process using scanning electron microscopy (SEM), X-ray diffraction (XRD) and Fourier Transform Infrared (FTIR) analysis, and the changes in chemical composition was also monitored.
26949055	4	20	theme	X-ray	745:749	arg1	diffraction					751:761	X-ray diffraction	745:761	X-ray diffraction (XRD)	745:767	Changes in structural properties of the biomass were studied in relation to the pretreatment process using scanning electron microscopy (SEM), X-ray diffraction (XRD) and Fourier Transform Infrared (FTIR) analysis, and the changes in chemical composition was also monitored.
26949055	4	20	theme	X-ray	745:749	arg1	XRD					764:766	XRD	764:766	XRD	764:766	Changes in structural properties of the biomass were studied in relation to the pretreatment process using scanning electron microscopy (SEM), X-ray diffraction (XRD) and Fourier Transform Infrared (FTIR) analysis, and the changes in chemical composition was also monitored.
26949055	1	21	theme	pretreatment	168:179	arg1	strategy					181:188	a sono-assisted acid pretreatment strategy	147:188	a sono-assisted acid pretreatment strategy for the effective removal of lignin and hemicelluloses	147:243	The objective of the present study was to develop a sono-assisted acid pretreatment strategy for the effective removal of lignin and hemicelluloses and to improve the sugar yield from chili post harvest residue.
26949055	1	22	theme	sugar	264:268	arg1	yield					270:274	the sugar yield	260:274	the sugar yield from chili post harvest residue	260:306	The objective of the present study was to develop a sono-assisted acid pretreatment strategy for the effective removal of lignin and hemicelluloses and to improve the sugar yield from chili post harvest residue.
26949055	0	23	theme	acid	22:25	arg1	pretreatment					27:38	A novel sono-assisted acid pretreatment	0:38	A novel sono-assisted acid pretreatment of chili post harvest residue for bioethanol production.	0:95	A novel sono-assisted acid pretreatment of chili post harvest residue for bioethanol production.
26949055	6	24	theme	fermentation	1075:1086	arg1	efficiency					1088:1097	a fermentation efficiency	1073:1097	a fermentation efficiency of 71.03%	1073:1107	Fermentation of the non-detoxified hydrolysate yielded 2.14% of bioethanol with a fermentation efficiency of 71.03%.
26949055	5	25	theme	novel	919:923	arg1	method					925:930	the optimized novel method	905:930	the optimized novel method	905:930	The biomass pretreated with the optimized novel method yielded 0.465g/g of reducing sugars on enzymatic hydrolysis.
26949055	6	26	theme	bioethanol	1057:1066	arg1	bioethanol					1057:1066	bioethanol	1057:1066	bioethanol	1057:1066	Fermentation of the non-detoxified hydrolysate yielded 2.14% of bioethanol with a fermentation efficiency of 71.03%.
26949055	6	26	theme	bioethanol	1057:1066	arg1	%					1052:1052	2.14%	1048:1052	2.14% of bioethanol	1048:1066	Fermentation of the non-detoxified hydrolysate yielded 2.14% of bioethanol with a fermentation efficiency of 71.03%.
26949055	0	27	theme	sono-assisted	8:20	arg1	pretreatment					27:38	A novel sono-assisted acid pretreatment	0:38	A novel sono-assisted acid pretreatment of chili post harvest residue for bioethanol production.	0:95	A novel sono-assisted acid pretreatment of chili post harvest residue for bioethanol production.
26949055	3	28	theme	propionic	574:582	arg1	acid					584:587	propionic acid	574:587	propionic acid	574:587	Inhibitor analysis of the hydrolyzate revealed that major fermentation inhibitors like furfural, hydroxymethyl furfural and organic acids like citric acid, succinic acid and propionic acid were absent.
26949055	4	29	theme	biomass	642:648	arg1	properties					624:633	structural properties	613:633	structural properties of the biomass	613:648	Changes in structural properties of the biomass were studied in relation to the pretreatment process using scanning electron microscopy (SEM), X-ray diffraction (XRD) and Fourier Transform Infrared (FTIR) analysis, and the changes in chemical composition was also monitored.
26949055	4	30	theme	pretreatment	682:693	arg1	process					695:701	the pretreatment process	678:701	the pretreatment process	678:701	Changes in structural properties of the biomass were studied in relation to the pretreatment process using scanning electron microscopy (SEM), X-ray diffraction (XRD) and Fourier Transform Infrared (FTIR) analysis, and the changes in chemical composition was also monitored.
26949055	1	31	theme	effective	198:206	arg1	removal					208:214	the effective removal	194:214	the effective removal of lignin and hemicelluloses	194:243	The objective of the present study was to develop a sono-assisted acid pretreatment strategy for the effective removal of lignin and hemicelluloses and to improve the sugar yield from chili post harvest residue.
26949055	3	32	theme	fermentation	458:469	arg1	inhibitors					471:480	major fermentation inhibitors	452:480	major fermentation inhibitors like furfural, hydroxymethyl furfural and organic acids like citric acid, succinic acid and propionic acid	452:587	Inhibitor analysis of the hydrolyzate revealed that major fermentation inhibitors like furfural, hydroxymethyl furfural and organic acids like citric acid, succinic acid and propionic acid were absent.
26949055	0	33	theme	post	49:52	arg1	residue					62:68	chili post harvest residue	43:68	chili post harvest residue	43:68	A novel sono-assisted acid pretreatment of chili post harvest residue for bioethanol production.
26949055	1	34	theme	harvest	292:298	arg1	residue					300:306	harvest residue	292:306	harvest residue	292:306	The objective of the present study was to develop a sono-assisted acid pretreatment strategy for the effective removal of lignin and hemicelluloses and to improve the sugar yield from chili post harvest residue.
26949055	0	35	theme	chili	43:47	arg1	residue					62:68	chili post harvest residue	43:68	chili post harvest residue	43:68	A novel sono-assisted acid pretreatment of chili post harvest residue for bioethanol production.
26949055	5	36	theme	optimized	909:917	arg1	method					925:930	the optimized novel method	905:930	the optimized novel method	905:930	The biomass pretreated with the optimized novel method yielded 0.465g/g of reducing sugars on enzymatic hydrolysis.
26949055	5	37	from	0.465g/g	940:947	arg1	hydrolysis					981:990	enzymatic hydrolysis	971:990	enzymatic hydrolysis	971:990	The biomass pretreated with the optimized novel method yielded 0.465g/g of reducing sugars on enzymatic hydrolysis.
26949055	4	38	theme	Infrared	791:798	arg1	analysis					807:814	Infrared (FTIR) analysis	791:814	Infrared (FTIR) analysis	791:814	Changes in structural properties of the biomass were studied in relation to the pretreatment process using scanning electron microscopy (SEM), X-ray diffraction (XRD) and Fourier Transform Infrared (FTIR) analysis, and the changes in chemical composition was also monitored.
26949055	6	39	theme	hydrolysate	1028:1038	arg1	Fermentation					993:1004	Fermentation	993:1004	Fermentation of the non-detoxified hydrolysate	993:1038	Fermentation of the non-detoxified hydrolysate yielded 2.14% of bioethanol with a fermentation efficiency of 71.03%.
26949055	1	40	theme	present	118:124	arg1	study					126:130	the present study	114:130	the present study	114:130	The objective of the present study was to develop a sono-assisted acid pretreatment strategy for the effective removal of lignin and hemicelluloses and to improve the sugar yield from chili post harvest residue.
26949055	5	41	theme	enzymatic	971:979	arg1	hydrolysis					981:990	enzymatic hydrolysis	971:990	enzymatic hydrolysis	971:990	The biomass pretreated with the optimized novel method yielded 0.465g/g of reducing sugars on enzymatic hydrolysis.
26949055	5	42	theme	reducing	952:959	arg1	sugars					961:966	reducing sugars	952:966	reducing sugars	952:966	The biomass pretreated with the optimized novel method yielded 0.465g/g of reducing sugars on enzymatic hydrolysis.
26949055	2	43	theme	Operational	309:319	arg1	parameters					321:330	Operational parameters	309:330	Operational parameters that affect the pretreatment efficiency	309:370	Operational parameters that affect the pretreatment efficiency were studied and optimized.
26949055	1	44	theme	lignin	219:224	arg1	removal					208:214	the effective removal	194:214	the effective removal of lignin and hemicelluloses	194:243	The objective of the present study was to develop a sono-assisted acid pretreatment strategy for the effective removal of lignin and hemicelluloses and to improve the sugar yield from chili post harvest residue.
26949055	0	45	theme	residue	62:68	arg1	pretreatment					27:38	A novel sono-assisted acid pretreatment	0:38	A novel sono-assisted acid pretreatment of chili post harvest residue for bioethanol production.	0:95	A novel sono-assisted acid pretreatment of chili post harvest residue for bioethanol production.
26949055	6	46	theme	%	1107:1107	arg1	efficiency					1088:1097	a fermentation efficiency	1073:1097	a fermentation efficiency of 71.03%	1073:1107	Fermentation of the non-detoxified hydrolysate yielded 2.14% of bioethanol with a fermentation efficiency of 71.03%.
26949055	4	47	dep	Fourier	773:779	arg1	Transform					781:789	Transform	781:789	Transform Infrared (FTIR) analysis	781:814	Changes in structural properties of the biomass were studied in relation to the pretreatment process using scanning electron microscopy (SEM), X-ray diffraction (XRD) and Fourier Transform Infrared (FTIR) analysis, and the changes in chemical composition was also monitored.
26949055	3	48	theme	hydroxymethyl	497:509	arg1	furfural					511:518	hydroxymethyl furfural	497:518	hydroxymethyl furfural	497:518	Inhibitor analysis of the hydrolyzate revealed that major fermentation inhibitors like furfural, hydroxymethyl furfural and organic acids like citric acid, succinic acid and propionic acid were absent.
26949055	6	49	theme	non-detoxified	1013:1026	arg1	hydrolysate					1028:1038	the non-detoxified hydrolysate	1009:1038	the non-detoxified hydrolysate	1009:1038	Fermentation of the non-detoxified hydrolysate yielded 2.14% of bioethanol with a fermentation efficiency of 71.03%.
26949055	0	50	theme	harvest	54:60	arg1	residue					62:68	chili post harvest residue	43:68	chili post harvest residue	43:68	A novel sono-assisted acid pretreatment of chili post harvest residue for bioethanol production.
26949055	3	51	theme	citric	543:548	arg1	acid					550:553	citric acid	543:553	citric acid	543:553	Inhibitor analysis of the hydrolyzate revealed that major fermentation inhibitors like furfural, hydroxymethyl furfural and organic acids like citric acid, succinic acid and propionic acid were absent.
26949055	1	52	theme	hemicelluloses	230:243	arg1	removal					208:214	the effective removal	194:214	the effective removal of lignin and hemicelluloses	194:243	The objective of the present study was to develop a sono-assisted acid pretreatment strategy for the effective removal of lignin and hemicelluloses and to improve the sugar yield from chili post harvest residue.
24712394	5	0	theme	peroxyl	881:887	arg1	hydroxyl					871:878	hydroxyl, peroxyl, and DPPH(•) radicals	871:909	hydroxyl	871:878	GPP-S exhibited scavenging capacities against hydroxyl, peroxyl, and DPPH(•) radicals in vitro.
24712394	2	1	theme	1.00:3.72:19.49:7.82	539:558	arg1	ratio					530:534	a molar ratio	522:534	a molar ratio of 1.00:3.72:19.49:7.82	522:558	The monosaccharide composition of GPP-S was determined as rhamnose, arabinose, glucose, and galactose with a molar ratio of 1.00:3.72:19.49:7.82.
24712394	5	2	theme	DPPH	894:897	arg1	radicals					902:909	hydroxyl, peroxyl, and DPPH(•) radicals	871:909	radicals	902:909	GPP-S exhibited scavenging capacities against hydroxyl, peroxyl, and DPPH(•) radicals in vitro.
24712394	1	3	theme	Da	244:245	arg1	mass					224:227	a molecular mass	212:227	a molecular mass of 1.2 × 10(6) Da	212:245	A novel polysaccharide (GPP-S), with a molecular mass of 1.2 × 10(6) Da, was isolated from the tetraploid Gynostemma pentaphyllum Makino by alkali extraction followed by purifications using DEAE and Sephacryl S-400 column chromatographies.
24712394	0	4	theme	potential	119:127	arg1	properties					163:172	its potential anti-inflammatory and antioxidant properties	115:172	its potential anti-inflammatory and antioxidant properties	115:172	Characterization of a novel alkali-soluble heteropolysaccharide from tetraploid Gynostemma pentaphyllum Makino and its potential anti-inflammatory and antioxidant properties.
24712394	0	5	from	Characterization	0:15	arg1	Makino					104:109	tetraploid Gynostemma pentaphyllum Makino	69:109	tetraploid Gynostemma pentaphyllum Makino	69:109	Characterization of a novel alkali-soluble heteropolysaccharide from tetraploid Gynostemma pentaphyllum Makino and its potential anti-inflammatory and antioxidant properties.
24712394	0	5	from	Characterization	0:15	arg1	properties					163:172	its potential anti-inflammatory and antioxidant properties	115:172	its potential anti-inflammatory and antioxidant properties	115:172	Characterization of a novel alkali-soluble heteropolysaccharide from tetraploid Gynostemma pentaphyllum Makino and its potential anti-inflammatory and antioxidant properties.
24712394	1	6	with	polysaccharide	183:196	arg1	mass					224:227	a molecular mass	212:227	a molecular mass of 1.2 × 10(6) Da	212:245	A novel polysaccharide (GPP-S), with a molecular mass of 1.2 × 10(6) Da, was isolated from the tetraploid Gynostemma pentaphyllum Makino by alkali extraction followed by purifications using DEAE and Sephacryl S-400 column chromatographies.
24712394	1	7	attach	isolated	252:259	arg2	GPP-S					199:203	GPP-S	199:203	GPP-S	199:203	A novel polysaccharide (GPP-S), with a molecular mass of 1.2 × 10(6) Da, was isolated from the tetraploid Gynostemma pentaphyllum Makino by alkali extraction followed by purifications using DEAE and Sephacryl S-400 column chromatographies.
24712394	1	7	attach	isolated	252:259	arg2	polysaccharide					183:196	A novel polysaccharide	175:196	A novel polysaccharide (GPP-S)	175:204	A novel polysaccharide (GPP-S), with a molecular mass of 1.2 × 10(6) Da, was isolated from the tetraploid Gynostemma pentaphyllum Makino by alkali extraction followed by purifications using DEAE and Sephacryl S-400 column chromatographies.
24712394	1	7	attach	isolated	252:259	arg1	Makino					305:310	Makino	305:310	Makino	305:310	A novel polysaccharide (GPP-S), with a molecular mass of 1.2 × 10(6) Da, was isolated from the tetraploid Gynostemma pentaphyllum Makino by alkali extraction followed by purifications using DEAE and Sephacryl S-400 column chromatographies.
24712394	7	8	theme	dietary	1143:1149	arg1	supplements					1151:1161	dietary supplements	1143:1161	dietary supplements	1143:1161	These results suggested that GPP-S could be developed as a bioactive ingredient for functional foods and dietary supplements.
24712394	6	9	from	IL-1β	961:965	arg1	cells					1031:1035	RAW 264.7 mouse macrophage cells	1004:1035	RAW 264.7 mouse macrophage cells	1004:1035	GPP-S also had inhibitory activities on IL-1β, IL-6, and COX-2 gene expressions in RAW 264.7 mouse macrophage cells.
24712394	6	10	theme	RAW	1004:1006	arg1	cells					1031:1035	RAW 264.7 mouse macrophage cells	1004:1035	RAW 264.7 mouse macrophage cells	1004:1035	GPP-S also had inhibitory activities on IL-1β, IL-6, and COX-2 gene expressions in RAW 264.7 mouse macrophage cells.
24712394	0	11	theme	anti-inflammatory	129:145	arg1	properties					163:172	its potential anti-inflammatory and antioxidant properties	115:172	its potential anti-inflammatory and antioxidant properties	115:172	Characterization of a novel alkali-soluble heteropolysaccharide from tetraploid Gynostemma pentaphyllum Makino and its potential anti-inflammatory and antioxidant properties.
24712394	3	12	theme	-linked-glucose	687:701	arg1	branch					703:708	a (1→4,6)-linked-glucose branch	678:708	a (1→4,6)-linked-glucose branch	678:708	The structural analysis suggested that the backbone of GPP-S is (1→4)-linked-glucose and (1→6)-linked-galactose with a (1→4,6)-linked-glucose branch every six monosaccharide residues.
24712394	7	13	theme	bioactive	1097:1105	arg1	ingredient					1107:1116	a bioactive ingredient	1095:1116	a bioactive ingredient for functional foods and dietary supplements	1095:1161	These results suggested that GPP-S could be developed as a bioactive ingredient for functional foods and dietary supplements.
24712394	7	13	theme	bioactive	1097:1105	arg1	GPP-S					1067:1071	GPP-S	1067:1071	GPP-S	1067:1071	These results suggested that GPP-S could be developed as a bioactive ingredient for functional foods and dietary supplements.
24712394	6	14	theme	COX-2	978:982	arg1	expressions					989:999	COX-2 gene expressions	978:999	COX-2 gene expressions	978:999	GPP-S also had inhibitory activities on IL-1β, IL-6, and COX-2 gene expressions in RAW 264.7 mouse macrophage cells.
24712394	3	15	with	-linked-galactose	655:671	arg1	branch					703:708	a (1→4,6)-linked-glucose branch	678:708	a (1→4,6)-linked-glucose branch	678:708	The structural analysis suggested that the backbone of GPP-S is (1→4)-linked-glucose and (1→6)-linked-galactose with a (1→4,6)-linked-glucose branch every six monosaccharide residues.
24712394	6	16	from	IL-6	968:971	arg1	cells					1031:1035	RAW 264.7 mouse macrophage cells	1004:1035	RAW 264.7 mouse macrophage cells	1004:1035	GPP-S also had inhibitory activities on IL-1β, IL-6, and COX-2 gene expressions in RAW 264.7 mouse macrophage cells.
24712394	6	17	from	expressions	989:999	arg1	cells					1031:1035	RAW 264.7 mouse macrophage cells	1004:1035	RAW 264.7 mouse macrophage cells	1004:1035	GPP-S also had inhibitory activities on IL-1β, IL-6, and COX-2 gene expressions in RAW 264.7 mouse macrophage cells.
24712394	6	18	theme	macrophage	1020:1029	arg1	cells					1031:1035	RAW 264.7 mouse macrophage cells	1004:1035	RAW 264.7 mouse macrophage cells	1004:1035	GPP-S also had inhibitory activities on IL-1β, IL-6, and COX-2 gene expressions in RAW 264.7 mouse macrophage cells.
24712394	1	19	theme	S-400	384:388	arg1	chromatographies					397:412	Sephacryl S-400 column chromatographies	374:412	Sephacryl S-400 column chromatographies	374:412	A novel polysaccharide (GPP-S), with a molecular mass of 1.2 × 10(6) Da, was isolated from the tetraploid Gynostemma pentaphyllum Makino by alkali extraction followed by purifications using DEAE and Sephacryl S-400 column chromatographies.
24712394	0	20	theme	novel	22:26	arg1	heteropolysaccharide					43:62	a novel alkali-soluble heteropolysaccharide	20:62	a novel alkali-soluble heteropolysaccharide from tetraploid Gynostemma pentaphyllum Makino and its potential anti-inflammatory and antioxidant properties	20:172	Characterization of a novel alkali-soluble heteropolysaccharide from tetraploid Gynostemma pentaphyllum Makino and its potential anti-inflammatory and antioxidant properties.
24712394	6	21	theme	inhibitory	936:945	arg1	activities					947:956	inhibitory activities	936:956	inhibitory activities	936:956	GPP-S also had inhibitory activities on IL-1β, IL-6, and COX-2 gene expressions in RAW 264.7 mouse macrophage cells.
24712394	1	22	theme	molecular	214:222	arg1	mass					224:227	a molecular mass	212:227	a molecular mass of 1.2 × 10(6) Da	212:245	A novel polysaccharide (GPP-S), with a molecular mass of 1.2 × 10(6) Da, was isolated from the tetraploid Gynostemma pentaphyllum Makino by alkali extraction followed by purifications using DEAE and Sephacryl S-400 column chromatographies.
24712394	7	23	theme	functional	1122:1131	arg1	foods					1133:1137	functional foods	1122:1137	functional foods	1122:1137	These results suggested that GPP-S could be developed as a bioactive ingredient for functional foods and dietary supplements.
24712394	3	24	theme	monosaccharide	720:733	arg1	backbone					604:611	the backbone	600:611	the backbone of GPP-S	600:620	The structural analysis suggested that the backbone of GPP-S is (1→4)-linked-glucose and (1→6)-linked-galactose with a (1→4,6)-linked-glucose branch every six monosaccharide residues.
24712394	3	24	theme	monosaccharide	720:733	arg1	residues					735:742	every six monosaccharide residues	710:742	(1→4)-linked-glucose and (1→6)-linked-galactose with a (1→4,6)-linked-glucose branch every six monosaccharide residues	625:742	The structural analysis suggested that the backbone of GPP-S is (1→4)-linked-glucose and (1→6)-linked-galactose with a (1→4,6)-linked-glucose branch every six monosaccharide residues.
24712394	3	24	theme	monosaccharide	720:733	arg1	-linked-glucose					630:644	-linked-glucose	630:644	-linked-glucose	630:644	The structural analysis suggested that the backbone of GPP-S is (1→4)-linked-glucose and (1→6)-linked-galactose with a (1→4,6)-linked-glucose branch every six monosaccharide residues.
24712394	0	25	theme	antioxidant	151:161	arg1	properties					163:172	its potential anti-inflammatory and antioxidant properties	115:172	its potential anti-inflammatory and antioxidant properties	115:172	Characterization of a novel alkali-soluble heteropolysaccharide from tetraploid Gynostemma pentaphyllum Makino and its potential anti-inflammatory and antioxidant properties.
24712394	1	26	theme	Sephacryl	374:382	arg1	chromatographies					397:412	Sephacryl S-400 column chromatographies	374:412	Sephacryl S-400 column chromatographies	374:412	A novel polysaccharide (GPP-S), with a molecular mass of 1.2 × 10(6) Da, was isolated from the tetraploid Gynostemma pentaphyllum Makino by alkali extraction followed by purifications using DEAE and Sephacryl S-400 column chromatographies.
24712394	3	27	theme	structural	565:574	arg1	analysis					576:583	The structural analysis	561:583	The structural analysis	561:583	The structural analysis suggested that the backbone of GPP-S is (1→4)-linked-glucose and (1→6)-linked-galactose with a (1→4,6)-linked-glucose branch every six monosaccharide residues.
24712394	2	28	theme	monosaccharide	419:432	arg1	rhamnose					473:480	rhamnose	473:480	rhamnose	473:480	The monosaccharide composition of GPP-S was determined as rhamnose, arabinose, glucose, and galactose with a molar ratio of 1.00:3.72:19.49:7.82.
24712394	2	28	theme	monosaccharide	419:432	arg1	arabinose					483:491	arabinose	483:491	arabinose	483:491	The monosaccharide composition of GPP-S was determined as rhamnose, arabinose, glucose, and galactose with a molar ratio of 1.00:3.72:19.49:7.82.
24712394	2	28	theme	monosaccharide	419:432	arg1	glucose					494:500	glucose	494:500	glucose	494:500	The monosaccharide composition of GPP-S was determined as rhamnose, arabinose, glucose, and galactose with a molar ratio of 1.00:3.72:19.49:7.82.
24712394	2	28	theme	monosaccharide	419:432	arg1	galactose					507:515	galactose	507:515	galactose	507:515	The monosaccharide composition of GPP-S was determined as rhamnose, arabinose, glucose, and galactose with a molar ratio of 1.00:3.72:19.49:7.82.
24712394	2	28	theme	monosaccharide	419:432	arg1	composition					434:444	The monosaccharide composition	415:444	The monosaccharide composition of GPP-S	415:453	The monosaccharide composition of GPP-S was determined as rhamnose, arabinose, glucose, and galactose with a molar ratio of 1.00:3.72:19.49:7.82.
24712394	0	29	theme	heteropolysaccharide	43:62	arg1	Characterization					0:15	Characterization	0:15	Characterization of a novel alkali-soluble heteropolysaccharide from tetraploid Gynostemma pentaphyllum Makino and its potential anti-inflammatory and antioxidant properties.	0:173	Characterization of a novel alkali-soluble heteropolysaccharide from tetraploid Gynostemma pentaphyllum Makino and its potential anti-inflammatory and antioxidant properties.
24712394	0	30	from	Makino	104:109	arg1	heteropolysaccharide					43:62	a novel alkali-soluble heteropolysaccharide	20:62	a novel alkali-soluble heteropolysaccharide from tetraploid Gynostemma pentaphyllum Makino and its potential anti-inflammatory and antioxidant properties	20:172	Characterization of a novel alkali-soluble heteropolysaccharide from tetraploid Gynostemma pentaphyllum Makino and its potential anti-inflammatory and antioxidant properties.
24712394	0	30	from	Makino	104:109	arg1	Characterization					0:15	Characterization	0:15	Characterization of a novel alkali-soluble heteropolysaccharide from tetraploid Gynostemma pentaphyllum Makino and its potential anti-inflammatory and antioxidant properties.	0:173	Characterization of a novel alkali-soluble heteropolysaccharide from tetraploid Gynostemma pentaphyllum Makino and its potential anti-inflammatory and antioxidant properties.
24712394	6	31	theme	mouse	1014:1018	arg1	cells					1031:1035	RAW 264.7 mouse macrophage cells	1004:1035	RAW 264.7 mouse macrophage cells	1004:1035	GPP-S also had inhibitory activities on IL-1β, IL-6, and COX-2 gene expressions in RAW 264.7 mouse macrophage cells.
24712394	0	32	theme	alkali-soluble	28:41	arg1	heteropolysaccharide					43:62	a novel alkali-soluble heteropolysaccharide	20:62	a novel alkali-soluble heteropolysaccharide from tetraploid Gynostemma pentaphyllum Makino and its potential anti-inflammatory and antioxidant properties	20:172	Characterization of a novel alkali-soluble heteropolysaccharide from tetraploid Gynostemma pentaphyllum Makino and its potential anti-inflammatory and antioxidant properties.
24712394	1	33	theme	column	390:395	arg1	chromatographies					397:412	Sephacryl S-400 column chromatographies	374:412	Sephacryl S-400 column chromatographies	374:412	A novel polysaccharide (GPP-S), with a molecular mass of 1.2 × 10(6) Da, was isolated from the tetraploid Gynostemma pentaphyllum Makino by alkali extraction followed by purifications using DEAE and Sephacryl S-400 column chromatographies.
24712394	2	34	theme	GPP-S	449:453	arg1	rhamnose					473:480	rhamnose	473:480	rhamnose	473:480	The monosaccharide composition of GPP-S was determined as rhamnose, arabinose, glucose, and galactose with a molar ratio of 1.00:3.72:19.49:7.82.
24712394	2	34	theme	GPP-S	449:453	arg1	arabinose					483:491	arabinose	483:491	arabinose	483:491	The monosaccharide composition of GPP-S was determined as rhamnose, arabinose, glucose, and galactose with a molar ratio of 1.00:3.72:19.49:7.82.
24712394	2	34	theme	GPP-S	449:453	arg1	glucose					494:500	glucose	494:500	glucose	494:500	The monosaccharide composition of GPP-S was determined as rhamnose, arabinose, glucose, and galactose with a molar ratio of 1.00:3.72:19.49:7.82.
24712394	2	34	theme	GPP-S	449:453	arg1	galactose					507:515	galactose	507:515	galactose	507:515	The monosaccharide composition of GPP-S was determined as rhamnose, arabinose, glucose, and galactose with a molar ratio of 1.00:3.72:19.49:7.82.
24712394	2	34	theme	GPP-S	449:453	arg1	composition					434:444	The monosaccharide composition	415:444	The monosaccharide composition of GPP-S	415:453	The monosaccharide composition of GPP-S was determined as rhamnose, arabinose, glucose, and galactose with a molar ratio of 1.00:3.72:19.49:7.82.
24712394	5	35	theme	scavenging	841:850	arg1	capacities					852:861	scavenging capacities	841:861	scavenging capacities against hydroxyl, peroxyl, and DPPH(•) radicals	841:909	GPP-S exhibited scavenging capacities against hydroxyl, peroxyl, and DPPH(•) radicals in vitro.
24712394	0	36	theme	tetraploid	69:78	arg1	Makino					104:109	tetraploid Gynostemma pentaphyllum Makino	69:109	tetraploid Gynostemma pentaphyllum Makino	69:109	Characterization of a novel alkali-soluble heteropolysaccharide from tetraploid Gynostemma pentaphyllum Makino and its potential anti-inflammatory and antioxidant properties.
24712394	2	37	theme	molar	524:528	arg1	ratio					530:534	a molar ratio	522:534	a molar ratio of 1.00:3.72:19.49:7.82	522:558	The monosaccharide composition of GPP-S was determined as rhamnose, arabinose, glucose, and galactose with a molar ratio of 1.00:3.72:19.49:7.82.
24712394	1	38	dep	10	238:239	arg1	6					241:241	6	241:241	6	241:241	A novel polysaccharide (GPP-S), with a molecular mass of 1.2 × 10(6) Da, was isolated from the tetraploid Gynostemma pentaphyllum Makino by alkali extraction followed by purifications using DEAE and Sephacryl S-400 column chromatographies.
24712394	3	39	theme	1→4,6	681:685	arg1	branch					703:708	a (1→4,6)-linked-glucose branch	678:708	a (1→4,6)-linked-glucose branch	678:708	The structural analysis suggested that the backbone of GPP-S is (1→4)-linked-glucose and (1→6)-linked-galactose with a (1→4,6)-linked-glucose branch every six monosaccharide residues.
24712394	4	40	theme	other	802:806	arg1	monosaccharides					808:822	other monosaccharides	802:822	other monosaccharides	802:822	The terminals were 1-)-α-arabinose, glucuronic acid, and other monosaccharides.
24712394	5	41	theme	•	899:899	arg1	radicals					902:909	hydroxyl, peroxyl, and DPPH(•) radicals	871:909	radicals	902:909	GPP-S exhibited scavenging capacities against hydroxyl, peroxyl, and DPPH(•) radicals in vitro.
24712394	6	42	contain	had	932:934	arg2	activities					947:956	inhibitory activities	936:956	inhibitory activities	936:956	GPP-S also had inhibitory activities on IL-1β, IL-6, and COX-2 gene expressions in RAW 264.7 mouse macrophage cells.
24712394	6	42	contain	had	932:934	arg1	GPP-S					921:925	GPP-S	921:925	GPP-S	921:925	GPP-S also had inhibitory activities on IL-1β, IL-6, and COX-2 gene expressions in RAW 264.7 mouse macrophage cells.
24712394	6	43	theme	gene	984:987	arg1	expressions					989:999	COX-2 gene expressions	978:999	COX-2 gene expressions	978:999	GPP-S also had inhibitory activities on IL-1β, IL-6, and COX-2 gene expressions in RAW 264.7 mouse macrophage cells.
24712394	3	44	dep	-linked-glucose	630:644	arg1	backbone					604:611	the backbone	600:611	the backbone of GPP-S	600:620	The structural analysis suggested that the backbone of GPP-S is (1→4)-linked-glucose and (1→6)-linked-galactose with a (1→4,6)-linked-glucose branch every six monosaccharide residues.
24712394	3	44	dep	-linked-glucose	630:644	arg1	residues					735:742	every six monosaccharide residues	710:742	(1→4)-linked-glucose and (1→6)-linked-galactose with a (1→4,6)-linked-glucose branch every six monosaccharide residues	625:742	The structural analysis suggested that the backbone of GPP-S is (1→4)-linked-glucose and (1→6)-linked-galactose with a (1→4,6)-linked-glucose branch every six monosaccharide residues.
24712394	3	44	dep	-linked-glucose	630:644	arg1	-linked-glucose					630:644	-linked-glucose	630:644	-linked-glucose	630:644	The structural analysis suggested that the backbone of GPP-S is (1→4)-linked-glucose and (1→6)-linked-galactose with a (1→4,6)-linked-glucose branch every six monosaccharide residues.
24712394	0	45	theme	pentaphyllum	91:102	arg1	Makino					104:109	tetraploid Gynostemma pentaphyllum Makino	69:109	tetraploid Gynostemma pentaphyllum Makino	69:109	Characterization of a novel alkali-soluble heteropolysaccharide from tetraploid Gynostemma pentaphyllum Makino and its potential anti-inflammatory and antioxidant properties.
24712394	3	46	theme	GPP-S	616:620	arg1	backbone					604:611	the backbone	600:611	the backbone of GPP-S	600:620	The structural analysis suggested that the backbone of GPP-S is (1→4)-linked-glucose and (1→6)-linked-galactose with a (1→4,6)-linked-glucose branch every six monosaccharide residues.
24712394	3	46	theme	GPP-S	616:620	arg1	-linked-glucose					630:644	-linked-glucose	630:644	-linked-glucose	630:644	The structural analysis suggested that the backbone of GPP-S is (1→4)-linked-glucose and (1→6)-linked-galactose with a (1→4,6)-linked-glucose branch every six monosaccharide residues.
24712394	3	46	theme	GPP-S	616:620	arg1	residues					735:742	every six monosaccharide residues	710:742	(1→4)-linked-glucose and (1→6)-linked-galactose with a (1→4,6)-linked-glucose branch every six monosaccharide residues	625:742	The structural analysis suggested that the backbone of GPP-S is (1→4)-linked-glucose and (1→6)-linked-galactose with a (1→4,6)-linked-glucose branch every six monosaccharide residues.
24712394	1	47	theme	novel	177:181	arg1	polysaccharide					183:196	A novel polysaccharide	175:196	A novel polysaccharide (GPP-S)	175:204	A novel polysaccharide (GPP-S), with a molecular mass of 1.2 × 10(6) Da, was isolated from the tetraploid Gynostemma pentaphyllum Makino by alkali extraction followed by purifications using DEAE and Sephacryl S-400 column chromatographies.
24712394	1	47	theme	novel	177:181	arg1	GPP-S					199:203	GPP-S	199:203	GPP-S	199:203	A novel polysaccharide (GPP-S), with a molecular mass of 1.2 × 10(6) Da, was isolated from the tetraploid Gynostemma pentaphyllum Makino by alkali extraction followed by purifications using DEAE and Sephacryl S-400 column chromatographies.
24712394	0	48	from	properties	163:172	arg1	heteropolysaccharide					43:62	a novel alkali-soluble heteropolysaccharide	20:62	a novel alkali-soluble heteropolysaccharide from tetraploid Gynostemma pentaphyllum Makino and its potential anti-inflammatory and antioxidant properties	20:172	Characterization of a novel alkali-soluble heteropolysaccharide from tetraploid Gynostemma pentaphyllum Makino and its potential anti-inflammatory and antioxidant properties.
24712394	0	48	from	properties	163:172	arg1	Characterization					0:15	Characterization	0:15	Characterization of a novel alkali-soluble heteropolysaccharide from tetraploid Gynostemma pentaphyllum Makino and its potential anti-inflammatory and antioxidant properties.	0:173	Characterization of a novel alkali-soluble heteropolysaccharide from tetraploid Gynostemma pentaphyllum Makino and its potential anti-inflammatory and antioxidant properties.
24712394	0	49	theme	Gynostemma	80:89	arg1	Makino					104:109	tetraploid Gynostemma pentaphyllum Makino	69:109	tetraploid Gynostemma pentaphyllum Makino	69:109	Characterization of a novel alkali-soluble heteropolysaccharide from tetraploid Gynostemma pentaphyllum Makino and its potential anti-inflammatory and antioxidant properties.
24712394	1	50	theme	alkali	315:320	arg1	extraction					322:331	alkali extraction	315:331	alkali extraction followed by purifications using DEAE and Sephacryl S-400 column chromatographies	315:412	A novel polysaccharide (GPP-S), with a molecular mass of 1.2 × 10(6) Da, was isolated from the tetraploid Gynostemma pentaphyllum Makino by alkali extraction followed by purifications using DEAE and Sephacryl S-400 column chromatographies.
24712394	4	51	theme	glucuronic	781:790	arg1	acid					792:795	glucuronic acid	781:795	glucuronic acid	781:795	The terminals were 1-)-α-arabinose, glucuronic acid, and other monosaccharides.
24530338	8	0	theme	macrophage	1287:1296	arg1	functions					1298:1306	macrophage functions	1287:1306	macrophage functions	1287:1306	Taken together, our results demonstrate that LBPF4 can enhance T, B cells and macrophages functions, but LBPF4-OL can only enhance B cells and macrophage functions.
24530338	0	1	from	comparison	18:27	arg1	Lycium					87:92	Lycium	87:92	Lycium	87:92	Immune activities comparison of polysaccharide and polysaccharide-protein complex from Lycium barbarum L. Lycium barbarum L., known as wolfberry, is an important Chinese herbal medicine.
24530338	3	2	theme	molecular	496:504	arg1	weight					506:511	LBPF4-OL molecular weight	487:511	LBPF4-OL molecular weight	487:511	LBPF4-OL molecular weight was 181 kDa, as determined by high-performance gel-permeation chromatography (HPGPC).
24530338	2	3	theme	NMR	471:473	arg1	techniques					475:484	NMR techniques	471:484	NMR techniques	471:484	The monosaccharide and amino acid composition of LBPF4 and LBPF4-OL was elucidated with fractional acid hydrolization, GC/MC and NMR techniques.
24530338	1	4	theme	barbarum	330:337	arg1	L					339:339	L. barbarum L	327:339	L. barbarum L	327:339	In the research, we purified water-soluble polysaccharide-protein complex (LBPF4) and polysaccharide (LBPF4-OL) from the fruiting bodies of L. barbarum L.
24530338	0	5	theme	herbal	170:175	arg1	comparison					18:27	Immune activities comparison	0:27	Immune activities comparison	0:27	Immune activities comparison of polysaccharide and polysaccharide-protein complex from Lycium barbarum L. Lycium barbarum L., known as wolfberry, is an important Chinese herbal medicine.
24530338	0	5	theme	herbal	170:175	arg1	medicine					177:184	an important Chinese herbal medicine	149:184	an important Chinese herbal medicine	149:184	Immune activities comparison of polysaccharide and polysaccharide-protein complex from Lycium barbarum L. Lycium barbarum L., known as wolfberry, is an important Chinese herbal medicine.
24530338	8	6	theme	T	1207:1207	arg1	functions					1234:1242	T, B cells and macrophages functions	1207:1242	T, B cells and macrophages functions	1207:1242	Taken together, our results demonstrate that LBPF4 can enhance T, B cells and macrophages functions, but LBPF4-OL can only enhance B cells and macrophage functions.
24530338	4	7	theme	B	686:686	arg1	cells					688:692	B cells	686:692	B cells	686:692	In vitro assay, we found that LBPF4 induced splenocyte proliferations depended on both B cells and T cells, but LBPF4-OL induced splenocyte proliferations mainly depended on B cells.
24530338	8	8	theme	cells	1212:1216	arg1	functions					1234:1242	T, B cells and macrophages functions	1207:1242	T, B cells and macrophages functions	1207:1242	Taken together, our results demonstrate that LBPF4 can enhance T, B cells and macrophages functions, but LBPF4-OL can only enhance B cells and macrophage functions.
24530338	9	9	theme	lymphocytes	1378:1388	arg1	activity					1396:1403	lymphocytes NF-κB activity	1378:1403	lymphocytes NF-κB activity	1378:1403	This is partly due to LBPF4 being able to more significantly enhance lymphocytes NF-κB activity.
24530338	1	10	theme	L	339:339	arg1	bodies					317:322	the fruiting bodies	304:322	the fruiting bodies of L. barbarum L	304:339	In the research, we purified water-soluble polysaccharide-protein complex (LBPF4) and polysaccharide (LBPF4-OL) from the fruiting bodies of L. barbarum L.
24530338	4	11	theme	induced	720:726	arg1	proliferations					739:752	LBPF4-OL induced splenocyte proliferations	711:752	LBPF4-OL induced splenocyte proliferations	711:752	In vitro assay, we found that LBPF4 induced splenocyte proliferations depended on both B cells and T cells, but LBPF4-OL induced splenocyte proliferations mainly depended on B cells.
24530338	9	12	theme	NF-κB	1390:1394	arg1	activity					1396:1403	lymphocytes NF-κB activity	1378:1403	lymphocytes NF-κB activity	1378:1403	This is partly due to LBPF4 being able to more significantly enhance lymphocytes NF-κB activity.
24530338	4	13	theme	LBPF4-OL	711:718	arg1	proliferations					739:752	LBPF4-OL induced splenocyte proliferations	711:752	LBPF4-OL induced splenocyte proliferations	711:752	In vitro assay, we found that LBPF4 induced splenocyte proliferations depended on both B cells and T cells, but LBPF4-OL induced splenocyte proliferations mainly depended on B cells.
24530338	7	14	theme	shift	1018:1022	arg1	EMSA					1031:1034	EMSA	1031:1034	EMSA	1031:1034	Furthermore, electrophoretic mobility shift assay (EMSA) studies suggest that LBPF4 100 μg/ml treatment can more effectively increase NF-κB activity than LBPF4-OL.
24530338	7	14	theme	shift	1018:1022	arg1	assay					1024:1028	electrophoretic mobility shift assay	993:1028	electrophoretic mobility shift assay (EMSA) studies	993:1043	Furthermore, electrophoretic mobility shift assay (EMSA) studies suggest that LBPF4 100 μg/ml treatment can more effectively increase NF-κB activity than LBPF4-OL.
24530338	6	15	theme	macrophage	955:964	arg1	phagocytosis					966:977	macrophage phagocytosis	955:977	macrophage phagocytosis	955:977	We also found that both LBPF4 and LBPF4-OL can enhance macrophage phagocytosis.
24530338	8	16	theme	macrophages	1222:1232	arg1	functions					1234:1242	T, B cells and macrophages functions	1207:1242	T, B cells and macrophages functions	1207:1242	Taken together, our results demonstrate that LBPF4 can enhance T, B cells and macrophages functions, but LBPF4-OL can only enhance B cells and macrophage functions.
24530338	4	17	theme	splenocyte	643:652	arg1	proliferations					654:667	LBPF4 induced splenocyte proliferations	629:667	LBPF4 induced splenocyte proliferations	629:667	In vitro assay, we found that LBPF4 induced splenocyte proliferations depended on both B cells and T cells, but LBPF4-OL induced splenocyte proliferations mainly depended on B cells.
24530338	5	18	theme	ELISA	782:786	arg1	results					788:794	ELISA results	782:794	ELISA results	782:794	ELISA results showed that both LBPF4 and LBPF4-OL significantly induced TNF-α, IL-1β and NO production on macrophage.
24530338	7	19	theme	LBPF4	1058:1062	arg1	treatment					1074:1082	LBPF4 100 μg/ml treatment	1058:1082	LBPF4 100 μg/ml treatment	1058:1082	Furthermore, electrophoretic mobility shift assay (EMSA) studies suggest that LBPF4 100 μg/ml treatment can more effectively increase NF-κB activity than LBPF4-OL.
24530338	0	20	dep	Lycium	106:111	arg1	L.					122:123	L. Lycium barbarum L.	103:123	Lycium barbarum L. Lycium barbarum L.	87:123	Immune activities comparison of polysaccharide and polysaccharide-protein complex from Lycium barbarum L. Lycium barbarum L., known as wolfberry, is an important Chinese herbal medicine.
24530338	4	21	theme	induced	635:641	arg1	proliferations					654:667	LBPF4 induced splenocyte proliferations	629:667	LBPF4 induced splenocyte proliferations	629:667	In vitro assay, we found that LBPF4 induced splenocyte proliferations depended on both B cells and T cells, but LBPF4-OL induced splenocyte proliferations mainly depended on B cells.
24530338	0	22	theme	activities	7:16	arg1	comparison					18:27	Immune activities comparison	0:27	Immune activities comparison	0:27	Immune activities comparison of polysaccharide and polysaccharide-protein complex from Lycium barbarum L. Lycium barbarum L., known as wolfberry, is an important Chinese herbal medicine.
24530338	0	22	theme	activities	7:16	arg1	medicine					177:184	an important Chinese herbal medicine	149:184	an important Chinese herbal medicine	149:184	Immune activities comparison of polysaccharide and polysaccharide-protein complex from Lycium barbarum L. Lycium barbarum L., known as wolfberry, is an important Chinese herbal medicine.
24530338	3	23	theme	gel-permeation	560:573	arg1	HPGPC					591:595	HPGPC	591:595	HPGPC	591:595	LBPF4-OL molecular weight was 181 kDa, as determined by high-performance gel-permeation chromatography (HPGPC).
24530338	3	23	theme	gel-permeation	560:573	arg1	chromatography					575:588	high-performance gel-permeation chromatography	543:588	high-performance gel-permeation chromatography (HPGPC)	543:596	LBPF4-OL molecular weight was 181 kDa, as determined by high-performance gel-permeation chromatography (HPGPC).
24530338	2	24	theme	acid	371:374	arg1	composition					376:386	The monosaccharide and amino acid composition	342:386	composition	376:386	The monosaccharide and amino acid composition of LBPF4 and LBPF4-OL was elucidated with fractional acid hydrolization, GC/MC and NMR techniques.
24530338	0	25	theme	Immune	0:5	arg1	comparison					18:27	Immune activities comparison	0:27	Immune activities comparison	0:27	Immune activities comparison of polysaccharide and polysaccharide-protein complex from Lycium barbarum L. Lycium barbarum L., known as wolfberry, is an important Chinese herbal medicine.
24530338	0	25	theme	Immune	0:5	arg1	medicine					177:184	an important Chinese herbal medicine	149:184	an important Chinese herbal medicine	149:184	Immune activities comparison of polysaccharide and polysaccharide-protein complex from Lycium barbarum L. Lycium barbarum L., known as wolfberry, is an important Chinese herbal medicine.
24530338	7	26	theme	μg/ml	1068:1072	arg1	treatment					1074:1082	LBPF4 100 μg/ml treatment	1058:1082	LBPF4 100 μg/ml treatment	1058:1082	Furthermore, electrophoretic mobility shift assay (EMSA) studies suggest that LBPF4 100 μg/ml treatment can more effectively increase NF-κB activity than LBPF4-OL.
24530338	2	27	theme	amino	365:369	arg1	composition					376:386	The monosaccharide and amino acid composition	342:386	composition	376:386	The monosaccharide and amino acid composition of LBPF4 and LBPF4-OL was elucidated with fractional acid hydrolization, GC/MC and NMR techniques.
24530338	0	28	dep	Lycium	87:92	arg1	barbarum					94:101	Lycium barbarum L. Lycium barbarum L.	87:123	Lycium barbarum L. Lycium barbarum L.	87:123	Immune activities comparison of polysaccharide and polysaccharide-protein complex from Lycium barbarum L. Lycium barbarum L., known as wolfberry, is an important Chinese herbal medicine.
24530338	0	28	dep	Lycium	87:92	arg1	Lycium					106:111	Lycium	106:111	Lycium	106:111	Immune activities comparison of polysaccharide and polysaccharide-protein complex from Lycium barbarum L. Lycium barbarum L., known as wolfberry, is an important Chinese herbal medicine.
24530338	2	29	theme	fractional	430:439	arg1	hydrolization					446:458	fractional acid hydrolization	430:458	fractional acid hydrolization	430:458	The monosaccharide and amino acid composition of LBPF4 and LBPF4-OL was elucidated with fractional acid hydrolization, GC/MC and NMR techniques.
24530338	3	30	theme	high-performance	543:558	arg1	HPGPC					591:595	HPGPC	591:595	HPGPC	591:595	LBPF4-OL molecular weight was 181 kDa, as determined by high-performance gel-permeation chromatography (HPGPC).
24530338	3	30	theme	high-performance	543:558	arg1	chromatography					575:588	high-performance gel-permeation chromatography	543:588	high-performance gel-permeation chromatography (HPGPC)	543:596	LBPF4-OL molecular weight was 181 kDa, as determined by high-performance gel-permeation chromatography (HPGPC).
24530338	2	31	theme	LBPF4-OL	401:408	arg1	composition					376:386	The monosaccharide and amino acid composition	342:386	composition	376:386	The monosaccharide and amino acid composition of LBPF4 and LBPF4-OL was elucidated with fractional acid hydrolization, GC/MC and NMR techniques.
24530338	2	31	theme	LBPF4-OL	401:408	arg1	monosaccharide					346:359	The monosaccharide and amino acid composition	342:386	monosaccharide	346:359	The monosaccharide and amino acid composition of LBPF4 and LBPF4-OL was elucidated with fractional acid hydrolization, GC/MC and NMR techniques.
24530338	0	32	theme	polysaccharide	32:45	arg1	comparison					18:27	Immune activities comparison	0:27	Immune activities comparison	0:27	Immune activities comparison of polysaccharide and polysaccharide-protein complex from Lycium barbarum L. Lycium barbarum L., known as wolfberry, is an important Chinese herbal medicine.
24530338	0	32	theme	polysaccharide	32:45	arg1	medicine					177:184	an important Chinese herbal medicine	149:184	an important Chinese herbal medicine	149:184	Immune activities comparison of polysaccharide and polysaccharide-protein complex from Lycium barbarum L. Lycium barbarum L., known as wolfberry, is an important Chinese herbal medicine.
24530338	0	33	from	Lycium	87:92	arg1	comparison					18:27	Immune activities comparison	0:27	Immune activities comparison	0:27	Immune activities comparison of polysaccharide and polysaccharide-protein complex from Lycium barbarum L. Lycium barbarum L., known as wolfberry, is an important Chinese herbal medicine.
24530338	0	33	from	Lycium	87:92	arg1	medicine					177:184	an important Chinese herbal medicine	149:184	an important Chinese herbal medicine	149:184	Immune activities comparison of polysaccharide and polysaccharide-protein complex from Lycium barbarum L. Lycium barbarum L., known as wolfberry, is an important Chinese herbal medicine.
24530338	0	33	from	Lycium	87:92	arg1	polysaccharide					32:45	polysaccharide	32:45	polysaccharide	32:45	Immune activities comparison of polysaccharide and polysaccharide-protein complex from Lycium barbarum L. Lycium barbarum L., known as wolfberry, is an important Chinese herbal medicine.
24530338	0	33	from	Lycium	87:92	arg1	complex					74:80	polysaccharide-protein complex	51:80	polysaccharide-protein complex	51:80	Immune activities comparison of polysaccharide and polysaccharide-protein complex from Lycium barbarum L. Lycium barbarum L., known as wolfberry, is an important Chinese herbal medicine.
24530338	7	34	theme	mobility	1009:1016	arg1	EMSA					1031:1034	EMSA	1031:1034	EMSA	1031:1034	Furthermore, electrophoretic mobility shift assay (EMSA) studies suggest that LBPF4 100 μg/ml treatment can more effectively increase NF-κB activity than LBPF4-OL.
24530338	7	34	theme	mobility	1009:1016	arg1	assay					1024:1028	electrophoretic mobility shift assay	993:1028	electrophoretic mobility shift assay (EMSA) studies	993:1043	Furthermore, electrophoretic mobility shift assay (EMSA) studies suggest that LBPF4 100 μg/ml treatment can more effectively increase NF-κB activity than LBPF4-OL.
24530338	2	35	theme	LBPF4	391:395	arg1	composition					376:386	The monosaccharide and amino acid composition	342:386	composition	376:386	The monosaccharide and amino acid composition of LBPF4 and LBPF4-OL was elucidated with fractional acid hydrolization, GC/MC and NMR techniques.
24530338	2	35	theme	LBPF4	391:395	arg1	monosaccharide					346:359	The monosaccharide and amino acid composition	342:386	monosaccharide	346:359	The monosaccharide and amino acid composition of LBPF4 and LBPF4-OL was elucidated with fractional acid hydrolization, GC/MC and NMR techniques.
24530338	0	36	theme	complex	74:80	arg1	comparison					18:27	Immune activities comparison	0:27	Immune activities comparison	0:27	Immune activities comparison of polysaccharide and polysaccharide-protein complex from Lycium barbarum L. Lycium barbarum L., known as wolfberry, is an important Chinese herbal medicine.
24530338	0	36	theme	complex	74:80	arg1	medicine					177:184	an important Chinese herbal medicine	149:184	an important Chinese herbal medicine	149:184	Immune activities comparison of polysaccharide and polysaccharide-protein complex from Lycium barbarum L. Lycium barbarum L., known as wolfberry, is an important Chinese herbal medicine.
24530338	1	37	theme	L.	327:328	arg1	L					339:339	L. barbarum L	327:339	L. barbarum L	327:339	In the research, we purified water-soluble polysaccharide-protein complex (LBPF4) and polysaccharide (LBPF4-OL) from the fruiting bodies of L. barbarum L.
24530338	7	38	theme	assay	1024:1028	arg1	studies					1037:1043	electrophoretic mobility shift assay (EMSA) studies	993:1043	electrophoretic mobility shift assay (EMSA) studies	993:1043	Furthermore, electrophoretic mobility shift assay (EMSA) studies suggest that LBPF4 100 μg/ml treatment can more effectively increase NF-κB activity than LBPF4-OL.
24530338	4	39	theme	T	698:698	arg1	cells					700:704	T cells	698:704	T cells	698:704	In vitro assay, we found that LBPF4 induced splenocyte proliferations depended on both B cells and T cells, but LBPF4-OL induced splenocyte proliferations mainly depended on B cells.
24530338	0	40	theme	polysaccharide-protein	51:72	arg1	complex					74:80	polysaccharide-protein complex	51:80	polysaccharide-protein complex	51:80	Immune activities comparison of polysaccharide and polysaccharide-protein complex from Lycium barbarum L. Lycium barbarum L., known as wolfberry, is an important Chinese herbal medicine.
24530338	1	41	theme	fruiting	308:315	arg1	bodies					317:322	the fruiting bodies	304:322	the fruiting bodies of L. barbarum L	304:339	In the research, we purified water-soluble polysaccharide-protein complex (LBPF4) and polysaccharide (LBPF4-OL) from the fruiting bodies of L. barbarum L.
24530338	7	42	theme	NF-κB	1114:1118	arg1	activity					1120:1127	NF-κB activity	1114:1127	NF-κB activity than LBPF4-OL	1114:1141	Furthermore, electrophoretic mobility shift assay (EMSA) studies suggest that LBPF4 100 μg/ml treatment can more effectively increase NF-κB activity than LBPF4-OL.
24530338	3	43	theme	LBPF4-OL	487:494	arg1	weight					506:511	LBPF4-OL molecular weight	487:511	LBPF4-OL molecular weight	487:511	LBPF4-OL molecular weight was 181 kDa, as determined by high-performance gel-permeation chromatography (HPGPC).
24530338	0	44	theme	Chinese	162:168	arg1	comparison					18:27	Immune activities comparison	0:27	Immune activities comparison	0:27	Immune activities comparison of polysaccharide and polysaccharide-protein complex from Lycium barbarum L. Lycium barbarum L., known as wolfberry, is an important Chinese herbal medicine.
24530338	0	44	theme	Chinese	162:168	arg1	medicine					177:184	an important Chinese herbal medicine	149:184	an important Chinese herbal medicine	149:184	Immune activities comparison of polysaccharide and polysaccharide-protein complex from Lycium barbarum L. Lycium barbarum L., known as wolfberry, is an important Chinese herbal medicine.
24530338	7	45	theme	electrophoretic	993:1007	arg1	EMSA					1031:1034	EMSA	1031:1034	EMSA	1031:1034	Furthermore, electrophoretic mobility shift assay (EMSA) studies suggest that LBPF4 100 μg/ml treatment can more effectively increase NF-κB activity than LBPF4-OL.
24530338	7	45	theme	electrophoretic	993:1007	arg1	assay					1024:1028	electrophoretic mobility shift assay	993:1028	electrophoretic mobility shift assay (EMSA) studies	993:1043	Furthermore, electrophoretic mobility shift assay (EMSA) studies suggest that LBPF4 100 μg/ml treatment can more effectively increase NF-κB activity than LBPF4-OL.
24530338	8	46	theme	B	1275:1275	arg1	cells					1277:1281	B cells	1275:1281	B cells	1275:1281	Taken together, our results demonstrate that LBPF4 can enhance T, B cells and macrophages functions, but LBPF4-OL can only enhance B cells and macrophage functions.
24530338	4	47	theme	splenocyte	728:737	arg1	proliferations					739:752	LBPF4-OL induced splenocyte proliferations	711:752	LBPF4-OL induced splenocyte proliferations	711:752	In vitro assay, we found that LBPF4 induced splenocyte proliferations depended on both B cells and T cells, but LBPF4-OL induced splenocyte proliferations mainly depended on B cells.
24530338	4	48	theme	vitro	602:606	arg1	assay					608:612	vitro assay	602:612	vitro assay	602:612	In vitro assay, we found that LBPF4 induced splenocyte proliferations depended on both B cells and T cells, but LBPF4-OL induced splenocyte proliferations mainly depended on B cells.
24530338	0	49	theme	important	152:160	arg1	comparison					18:27	Immune activities comparison	0:27	Immune activities comparison	0:27	Immune activities comparison of polysaccharide and polysaccharide-protein complex from Lycium barbarum L. Lycium barbarum L., known as wolfberry, is an important Chinese herbal medicine.
24530338	0	49	theme	important	152:160	arg1	medicine					177:184	an important Chinese herbal medicine	149:184	an important Chinese herbal medicine	149:184	Immune activities comparison of polysaccharide and polysaccharide-protein complex from Lycium barbarum L. Lycium barbarum L., known as wolfberry, is an important Chinese herbal medicine.
24530338	4	50	theme	B	773:773	arg1	cells					775:779	B cells	773:779	B cells	773:779	In vitro assay, we found that LBPF4 induced splenocyte proliferations depended on both B cells and T cells, but LBPF4-OL induced splenocyte proliferations mainly depended on B cells.
24530338	5	51	theme	NO	871:872	arg1	production					874:883	NO production	871:883	NO production	871:883	ELISA results showed that both LBPF4 and LBPF4-OL significantly induced TNF-α, IL-1β and NO production on macrophage.
24530338	1	52	theme	water-soluble	216:228	arg1	LBPF4					262:266	LBPF4	262:266	LBPF4	262:266	In the research, we purified water-soluble polysaccharide-protein complex (LBPF4) and polysaccharide (LBPF4-OL) from the fruiting bodies of L. barbarum L.
24530338	1	52	theme	water-soluble	216:228	arg1	complex					253:259	water-soluble polysaccharide-protein complex	216:259	water-soluble polysaccharide-protein complex (LBPF4)	216:267	In the research, we purified water-soluble polysaccharide-protein complex (LBPF4) and polysaccharide (LBPF4-OL) from the fruiting bodies of L. barbarum L.
24530338	8	53	theme	B	1210:1210	arg1	cells					1212:1216	B cells	1210:1216	B cells	1210:1216	Taken together, our results demonstrate that LBPF4 can enhance T, B cells and macrophages functions, but LBPF4-OL can only enhance B cells and macrophage functions.
24530338	1	54	theme	polysaccharide-protein	230:251	arg1	LBPF4					262:266	LBPF4	262:266	LBPF4	262:266	In the research, we purified water-soluble polysaccharide-protein complex (LBPF4) and polysaccharide (LBPF4-OL) from the fruiting bodies of L. barbarum L.
24530338	1	54	theme	polysaccharide-protein	230:251	arg1	complex					253:259	water-soluble polysaccharide-protein complex	216:259	water-soluble polysaccharide-protein complex (LBPF4)	216:267	In the research, we purified water-soluble polysaccharide-protein complex (LBPF4) and polysaccharide (LBPF4-OL) from the fruiting bodies of L. barbarum L.
24530338	2	55	theme	acid	441:444	arg1	hydrolization					446:458	fractional acid hydrolization	430:458	fractional acid hydrolization	430:458	The monosaccharide and amino acid composition of LBPF4 and LBPF4-OL was elucidated with fractional acid hydrolization, GC/MC and NMR techniques.
25106895	2	0	from	yield	212:216	arg1	144 h					256:260	144 h	256:260	144 h of submerged cultivation	256:285	Biomass yield 14.3 g/L and EPS synthesis 4.3 g/L in 144 h of submerged cultivation were achieved.
25106895	8	1	theme	laurentii	928:936	arg1	EPS					938:940	raw C. laurentii EPS	921:940	raw C. laurentii EPS	921:940	Molecular mass distribution of raw C. laurentii EPS was determined by SEC-MALS as 1.352.
25106895	4	2	theme	1 	403:404	arg1	%					418:418	1 % < pO2 < 10 %	403:418	1 % < pO2 < 10 %	403:418	For EPS formation, pH 3, 25 °C and low aeration (1 % < pO2 < 10 %) were advantageous, while cell growth optimum was at pH 6, 20 °C, and high aeration (pO2 > 30 %).
25106895	4	2	theme	1 	403:404	arg1	aeration					393:400	low aeration	389:400	low aeration (1 % < pO2 < 10 %)	389:419	For EPS formation, pH 3, 25 °C and low aeration (1 % < pO2 < 10 %) were advantageous, while cell growth optimum was at pH 6, 20 °C, and high aeration (pO2 > 30 %).
25106895	6	3	theme	polysaccharide	717:730	arg1	synthesis					732:740	GluAc containing polysaccharide synthesis	700:740	GluAc containing polysaccharide synthesis	700:740	Twenty-five degrees Celsius was optimal for GluAc containing polysaccharide synthesis, while lower temperature (15 °C) increased glucose content in EPS.
25106895	10	4	theme	wound	1120:1124	arg1	healing					1126:1132	excisional wound healing	1109:1132	excisional wound healing	1109:1132	In the in vivo experiments, EPS significantly improved excisional wound healing in healthy rats.
25106895	7	5	theme	little	856:861	arg1	effect					863:868	little effect	856:868	little effect	856:868	Aeration intensity and time of cultivation had little effect on EPS composition.
25106895	11	6	theme	C.	1176:1177	arg1	EPS					1189:1191	C. laurentii EPS	1176:1191	C. laurentii EPS	1176:1191	The results suggest that C. laurentii EPS is a promising biotechnological product and an advanced material for application in wound management.
25106895	11	6	theme	C.	1176:1177	arg1	product					1225:1231	a promising biotechnological product	1196:1231	a promising biotechnological product	1196:1231	The results suggest that C. laurentii EPS is a promising biotechnological product and an advanced material for application in wound management.
25106895	7	7	theme	EPS	873:875	arg1	composition					877:887	EPS composition	873:887	EPS composition	873:887	Aeration intensity and time of cultivation had little effect on EPS composition.
25106895	9	8	theme	row	983:985	arg1	EPS					987:989	The row EPS	979:989	The row EPS	979:989	The row EPS was composed of acidic glucuronoxylomannan for more than 85 %.
25106895	10	9	theme	excisional	1109:1118	arg1	healing					1126:1132	excisional wound healing	1109:1132	excisional wound healing	1109:1132	In the in vivo experiments, EPS significantly improved excisional wound healing in healthy rats.
25106895	11	10	theme	laurentii	1179:1187	arg1	EPS					1189:1191	C. laurentii EPS	1176:1191	C. laurentii EPS	1176:1191	The results suggest that C. laurentii EPS is a promising biotechnological product and an advanced material for application in wound management.
25106895	11	10	theme	laurentii	1179:1187	arg1	product					1225:1231	a promising biotechnological product	1196:1231	a promising biotechnological product	1196:1231	The results suggest that C. laurentii EPS is a promising biotechnological product and an advanced material for application in wound management.
25106895	1	11	from	production	165:174	arg1	bioreactor					179:188	bioreactor	179:188	bioreactor	179:188	Cryptococcus laurentii growth and extracellular polysaccharide (EPS) production in bioreactor were studied.
25106895	7	12	theme	cultivation	840:850	arg1	Aeration					809:816	Aeration	809:816	Aeration	809:816	Aeration intensity and time of cultivation had little effect on EPS composition.
25106895	7	12	theme	cultivation	840:850	arg1	time					832:835	time	832:835	time	832:835	Aeration intensity and time of cultivation had little effect on EPS composition.
25106895	8	13	theme	C.	925:926	arg1	EPS					938:940	raw C. laurentii EPS	921:940	raw C. laurentii EPS	921:940	Molecular mass distribution of raw C. laurentii EPS was determined by SEC-MALS as 1.352.
25106895	0	14	from	application	63:73	arg1	management					84:93	wound management	78:93	wound management	78:93	Cryptococcus laurentii extracellular biopolymer production for application in wound management.
25106895	5	15	theme	glucuronic	558:567	arg1	GluAc					575:579	GluAc	575:579	GluAc	575:579	As medium pH changed from pH 3 to pH 6, glucuronic acid (GluAc) content in EPS increased, while galactose, xylose, and glucose decreased.
25106895	5	15	theme	glucuronic	558:567	arg1	acid					569:572	glucuronic acid	558:572	glucuronic acid (GluAc) content in EPS	558:595	As medium pH changed from pH 3 to pH 6, glucuronic acid (GluAc) content in EPS increased, while galactose, xylose, and glucose decreased.
25106895	4	16	theme	%	405:405	arg1	%					418:418	1 % < pO2 < 10 %	403:418	1 % < pO2 < 10 %	403:418	For EPS formation, pH 3, 25 °C and low aeration (1 % < pO2 < 10 %) were advantageous, while cell growth optimum was at pH 6, 20 °C, and high aeration (pO2 > 30 %).
25106895	4	16	theme	%	405:405	arg1	aeration					393:400	low aeration	389:400	low aeration (1 % < pO2 < 10 %)	389:419	For EPS formation, pH 3, 25 °C and low aeration (1 % < pO2 < 10 %) were advantageous, while cell growth optimum was at pH 6, 20 °C, and high aeration (pO2 > 30 %).
25106895	8	17	theme	Molecular	890:898	arg1	distribution					905:916	Molecular mass distribution	890:916	Molecular mass distribution of raw C. laurentii EPS	890:940	Molecular mass distribution of raw C. laurentii EPS was determined by SEC-MALS as 1.352.
25106895	8	17	theme	Molecular	890:898	arg1	1.352					972:976	1.352	972:976	1.352	972:976	Molecular mass distribution of raw C. laurentii EPS was determined by SEC-MALS as 1.352.
25106895	2	18	from	4.3 g/L	245:251	arg1	144 h					256:260	144 h	256:260	144 h of submerged cultivation	256:285	Biomass yield 14.3 g/L and EPS synthesis 4.3 g/L in 144 h of submerged cultivation were achieved.
25106895	5	19	theme	acid	569:572	arg1	content					582:588	glucuronic acid (GluAc) content	558:588	glucuronic acid (GluAc) content in EPS	558:595	As medium pH changed from pH 3 to pH 6, glucuronic acid (GluAc) content in EPS increased, while galactose, xylose, and glucose decreased.
25106895	5	20	theme	medium	521:526	arg1	pH					528:529	medium pH	521:529	medium pH	521:529	As medium pH changed from pH 3 to pH 6, glucuronic acid (GluAc) content in EPS increased, while galactose, xylose, and glucose decreased.
25106895	1	21	theme	Cryptococcus	96:107	arg1	growth					119:124	Cryptococcus laurentii growth	96:124	Cryptococcus laurentii growth	96:124	Cryptococcus laurentii growth and extracellular polysaccharide (EPS) production in bioreactor were studied.
25106895	11	22	theme	advanced	1240:1247	arg1	material					1249:1256	an advanced material	1237:1256	an advanced material for application in wound management	1237:1292	The results suggest that C. laurentii EPS is a promising biotechnological product and an advanced material for application in wound management.
25106895	11	23	from	material	1249:1256	arg1	management					1283:1292	wound management	1277:1292	wound management	1277:1292	The results suggest that C. laurentii EPS is a promising biotechnological product and an advanced material for application in wound management.
25106895	6	24	theme	containing	706:715	arg1	synthesis					732:740	GluAc containing polysaccharide synthesis	700:740	GluAc containing polysaccharide synthesis	700:740	Twenty-five degrees Celsius was optimal for GluAc containing polysaccharide synthesis, while lower temperature (15 °C) increased glucose content in EPS.
25106895	1	25	theme	laurentii	109:117	arg1	growth					119:124	Cryptococcus laurentii growth	96:124	Cryptococcus laurentii growth	96:124	Cryptococcus laurentii growth and extracellular polysaccharide (EPS) production in bioreactor were studied.
25106895	3	26	contain	had	332:334	arg1	synthesis					306:314	EPS synthesis	302:314	EPS synthesis	302:314	EPS synthesis and cell growth had different optima.
25106895	3	26	contain	had	332:334	arg2	optima					346:351	different optima	336:351	different optima	336:351	EPS synthesis and cell growth had different optima.
25106895	3	26	contain	had	332:334	arg1	growth					325:330	cell growth	320:330	cell growth	320:330	EPS synthesis and cell growth had different optima.
25106895	7	27	contain	had	852:854	arg1	Aeration					809:816	Aeration	809:816	Aeration	809:816	Aeration intensity and time of cultivation had little effect on EPS composition.
25106895	7	27	contain	had	852:854	arg1	time					832:835	time	832:835	time	832:835	Aeration intensity and time of cultivation had little effect on EPS composition.
25106895	7	27	contain	had	852:854	arg2	effect					863:868	little effect	856:868	little effect	856:868	Aeration intensity and time of cultivation had little effect on EPS composition.
25106895	11	28	theme	promising	1198:1206	arg1	EPS					1189:1191	C. laurentii EPS	1176:1191	C. laurentii EPS	1176:1191	The results suggest that C. laurentii EPS is a promising biotechnological product and an advanced material for application in wound management.
25106895	11	28	theme	promising	1198:1206	arg1	product					1225:1231	a promising biotechnological product	1196:1231	a promising biotechnological product	1196:1231	The results suggest that C. laurentii EPS is a promising biotechnological product and an advanced material for application in wound management.
25106895	6	29	theme	GluAc	700:704	arg1	synthesis					732:740	GluAc containing polysaccharide synthesis	700:740	GluAc containing polysaccharide synthesis	700:740	Twenty-five degrees Celsius was optimal for GluAc containing polysaccharide synthesis, while lower temperature (15 °C) increased glucose content in EPS.
25106895	11	30	theme	biotechnological	1208:1223	arg1	EPS					1189:1191	C. laurentii EPS	1176:1191	C. laurentii EPS	1176:1191	The results suggest that C. laurentii EPS is a promising biotechnological product and an advanced material for application in wound management.
25106895	11	30	theme	biotechnological	1208:1223	arg1	product					1225:1231	a promising biotechnological product	1196:1231	a promising biotechnological product	1196:1231	The results suggest that C. laurentii EPS is a promising biotechnological product and an advanced material for application in wound management.
25106895	9	31	theme	acidic	1007:1012	arg1	glucuronoxylomannan					1014:1032	acidic glucuronoxylomannan	1007:1032	acidic glucuronoxylomannan for more than 85 %	1007:1051	The row EPS was composed of acidic glucuronoxylomannan for more than 85 %.
25106895	0	32	theme	biopolymer	37:46	arg1	production					48:57	extracellular biopolymer production	23:57	extracellular biopolymer production	23:57	Cryptococcus laurentii extracellular biopolymer production for application in wound management.
25106895	7	33	dep	Aeration	809:816	arg1	intensity					818:826	intensity	818:826	intensity	818:826	Aeration intensity and time of cultivation had little effect on EPS composition.
25106895	4	34	theme	low	389:391	arg1	%					418:418	1 % < pO2 < 10 %	403:418	1 % < pO2 < 10 %	403:418	For EPS formation, pH 3, 25 °C and low aeration (1 % < pO2 < 10 %) were advantageous, while cell growth optimum was at pH 6, 20 °C, and high aeration (pO2 > 30 %).
25106895	4	34	theme	low	389:391	arg1	aeration					393:400	low aeration	389:400	low aeration (1 % < pO2 < 10 %)	389:419	For EPS formation, pH 3, 25 °C and low aeration (1 % < pO2 < 10 %) were advantageous, while cell growth optimum was at pH 6, 20 °C, and high aeration (pO2 > 30 %).
25106895	6	35	theme	lower	749:753	arg1	15 °C					768:772	15 °C	768:772	15 °C	768:772	Twenty-five degrees Celsius was optimal for GluAc containing polysaccharide synthesis, while lower temperature (15 °C) increased glucose content in EPS.
25106895	6	35	theme	lower	749:753	arg1	temperature					755:765	lower temperature	749:765	lower temperature (15 °C)	749:773	Twenty-five degrees Celsius was optimal for GluAc containing polysaccharide synthesis, while lower temperature (15 °C) increased glucose content in EPS.
25106895	0	36	theme	extracellular	23:35	arg1	production					48:57	extracellular biopolymer production	23:57	extracellular biopolymer production	23:57	Cryptococcus laurentii extracellular biopolymer production for application in wound management.
25106895	1	37	theme	extracellular	130:142	arg1	EPS					160:162	EPS	160:162	EPS	160:162	Cryptococcus laurentii growth and extracellular polysaccharide (EPS) production in bioreactor were studied.
25106895	1	37	theme	extracellular	130:142	arg1	polysaccharide					144:157	extracellular polysaccharide	130:157	extracellular polysaccharide (EPS) production	130:174	Cryptococcus laurentii growth and extracellular polysaccharide (EPS) production in bioreactor were studied.
25106895	11	38	from	product	1225:1231	arg1	management					1283:1292	wound management	1277:1292	wound management	1277:1292	The results suggest that C. laurentii EPS is a promising biotechnological product and an advanced material for application in wound management.
25106895	2	39	theme	Biomass	204:210	arg1	yield					212:216	Biomass yield 14.3 g/L	204:225	Biomass yield 14.3 g/L	204:225	Biomass yield 14.3 g/L and EPS synthesis 4.3 g/L in 144 h of submerged cultivation were achieved.
25106895	11	40	theme	wound	1277:1281	arg1	management					1283:1292	wound management	1277:1292	wound management	1277:1292	The results suggest that C. laurentii EPS is a promising biotechnological product and an advanced material for application in wound management.
25106895	8	41	theme	raw	921:923	arg1	EPS					938:940	raw C. laurentii EPS	921:940	raw C. laurentii EPS	921:940	Molecular mass distribution of raw C. laurentii EPS was determined by SEC-MALS as 1.352.
25106895	3	42	theme	EPS	302:304	arg1	synthesis					306:314	EPS synthesis	302:314	EPS synthesis	302:314	EPS synthesis and cell growth had different optima.
25106895	4	43	theme	growth	451:456	arg1	optimum					458:464	cell growth optimum	446:464	cell growth optimum	446:464	For EPS formation, pH 3, 25 °C and low aeration (1 % < pO2 < 10 %) were advantageous, while cell growth optimum was at pH 6, 20 °C, and high aeration (pO2 > 30 %).
25106895	8	44	theme	EPS	938:940	arg1	distribution					905:916	Molecular mass distribution	890:916	Molecular mass distribution of raw C. laurentii EPS	890:940	Molecular mass distribution of raw C. laurentii EPS was determined by SEC-MALS as 1.352.
25106895	8	44	theme	EPS	938:940	arg1	1.352					972:976	1.352	972:976	1.352	972:976	Molecular mass distribution of raw C. laurentii EPS was determined by SEC-MALS as 1.352.
25106895	4	45	theme	high	490:493	arg1	aeration					495:502	high aeration	490:502	high aeration (pO2 > 30 %)	490:515	For EPS formation, pH 3, 25 °C and low aeration (1 % < pO2 < 10 %) were advantageous, while cell growth optimum was at pH 6, 20 °C, and high aeration (pO2 > 30 %).
25106895	4	45	theme	high	490:493	arg1	%					514:514	pO2 > 30 %	505:514	pO2 > 30 %	505:514	For EPS formation, pH 3, 25 °C and low aeration (1 % < pO2 < 10 %) were advantageous, while cell growth optimum was at pH 6, 20 °C, and high aeration (pO2 > 30 %).
25106895	8	46	theme	mass	900:903	arg1	distribution					905:916	Molecular mass distribution	890:916	Molecular mass distribution of raw C. laurentii EPS	890:940	Molecular mass distribution of raw C. laurentii EPS was determined by SEC-MALS as 1.352.
25106895	8	46	theme	mass	900:903	arg1	1.352					972:976	1.352	972:976	1.352	972:976	Molecular mass distribution of raw C. laurentii EPS was determined by SEC-MALS as 1.352.
25106895	4	47	theme	cell	446:449	arg1	optimum					458:464	cell growth optimum	446:464	cell growth optimum	446:464	For EPS formation, pH 3, 25 °C and low aeration (1 % < pO2 < 10 %) were advantageous, while cell growth optimum was at pH 6, 20 °C, and high aeration (pO2 > 30 %).
25106895	1	48	theme	polysaccharide	144:157	arg1	production					165:174	extracellular polysaccharide (EPS) production	130:174	extracellular polysaccharide (EPS) production	130:174	Cryptococcus laurentii growth and extracellular polysaccharide (EPS) production in bioreactor were studied.
25106895	10	49	dep	in	1061:1062	arg1	vivo					1064:1067	vivo	1064:1067	vivo	1064:1067	In the in vivo experiments, EPS significantly improved excisional wound healing in healthy rats.
25106895	3	50	theme	different	336:344	arg1	optima					346:351	different optima	336:351	different optima	336:351	EPS synthesis and cell growth had different optima.
25106895	2	51	theme	synthesis	235:243	arg1	4.3 g/L					245:251	EPS synthesis 4.3 g/L	231:251	EPS synthesis 4.3 g/L	231:251	Biomass yield 14.3 g/L and EPS synthesis 4.3 g/L in 144 h of submerged cultivation were achieved.
25106895	6	52	theme	glucose	785:791	arg1	content					793:799	glucose content	785:799	glucose content in EPS	785:806	Twenty-five degrees Celsius was optimal for GluAc containing polysaccharide synthesis, while lower temperature (15 °C) increased glucose content in EPS.
25106895	5	53	dep	pH 6	552:555	arg1	to					549:550	to	549:550	to	549:550	As medium pH changed from pH 3 to pH 6, glucuronic acid (GluAc) content in EPS increased, while galactose, xylose, and glucose decreased.
25106895	5	54	from	content	582:588	arg1	EPS					593:595	EPS	593:595	EPS	593:595	As medium pH changed from pH 3 to pH 6, glucuronic acid (GluAc) content in EPS increased, while galactose, xylose, and glucose decreased.
25106895	3	55	theme	cell	320:323	arg1	growth					325:330	cell growth	320:330	cell growth	320:330	EPS synthesis and cell growth had different optima.
25106895	2	56	theme	EPS	231:233	arg1	4.3 g/L					245:251	EPS synthesis 4.3 g/L	231:251	EPS synthesis 4.3 g/L	231:251	Biomass yield 14.3 g/L and EPS synthesis 4.3 g/L in 144 h of submerged cultivation were achieved.
25106895	6	57	theme	degrees	668:674	arg1	Celsius					676:682	Twenty-five degrees Celsius	656:682	Twenty-five degrees Celsius	656:682	Twenty-five degrees Celsius was optimal for GluAc containing polysaccharide synthesis, while lower temperature (15 °C) increased glucose content in EPS.
25106895	6	58	from	content	793:799	arg1	EPS					804:806	EPS	804:806	EPS	804:806	Twenty-five degrees Celsius was optimal for GluAc containing polysaccharide synthesis, while lower temperature (15 °C) increased glucose content in EPS.
25106895	6	59	theme	Twenty-five	656:666	arg1	Celsius					676:682	Twenty-five degrees Celsius	656:682	Twenty-five degrees Celsius	656:682	Twenty-five degrees Celsius was optimal for GluAc containing polysaccharide synthesis, while lower temperature (15 °C) increased glucose content in EPS.
25106895	10	60	theme	healthy	1137:1143	arg1	rats					1145:1148	healthy rats	1137:1148	healthy rats	1137:1148	In the in vivo experiments, EPS significantly improved excisional wound healing in healthy rats.
25106895	2	61	theme	cultivation	275:285	arg1	144 h					256:260	144 h	256:260	144 h of submerged cultivation	256:285	Biomass yield 14.3 g/L and EPS synthesis 4.3 g/L in 144 h of submerged cultivation were achieved.
25106895	1	62	from	growth	119:124	arg1	bioreactor					179:188	bioreactor	179:188	bioreactor	179:188	Cryptococcus laurentii growth and extracellular polysaccharide (EPS) production in bioreactor were studied.
25106895	0	63	theme	wound	78:82	arg1	management					84:93	wound management	78:93	wound management	78:93	Cryptococcus laurentii extracellular biopolymer production for application in wound management.
25106895	2	64	theme	submerged	265:273	arg1	cultivation					275:285	submerged cultivation	265:285	submerged cultivation	265:285	Biomass yield 14.3 g/L and EPS synthesis 4.3 g/L in 144 h of submerged cultivation were achieved.
25106895	4	65	theme	EPS	358:360	arg1	formation					362:370	EPS formation	358:370	EPS formation	358:370	For EPS formation, pH 3, 25 °C and low aeration (1 % < pO2 < 10 %) were advantageous, while cell growth optimum was at pH 6, 20 °C, and high aeration (pO2 > 30 %).
25106895	10	66	theme	in	1061:1062	arg1	experiments					1069:1079	the in vivo experiments	1057:1079	the in vivo experiments	1057:1079	In the in vivo experiments, EPS significantly improved excisional wound healing in healthy rats.
26887688	8	0	with	reaction	1108:1115	arg1	group					1175:1179	the amino group	1165:1179	the amino group of creatinine in the model reaction	1165:1215	Furthermore, the complex spatial structure of waxy rice starch disturbs the reaction of the glucosyl hydroxyl groups of glucose with the amino group of creatinine in the model reaction.
26887688	8	0	with	reaction	1108:1115	arg1	creatinine					1184:1193	creatinine	1184:1193	creatinine in the model reaction	1184:1215	Furthermore, the complex spatial structure of waxy rice starch disturbs the reaction of the glucosyl hydroxyl groups of glucose with the amino group of creatinine in the model reaction.
26887688	9	1	theme	SCA	1235:1237	arg1	pronounced					1248:1257	pronounced	1248:1257	pronounced	1248:1257	So the effect of SCA was more pronounced than that of waxy rice starch on suppressing PhIP formation.
26887688	9	1	theme	SCA	1235:1237	arg1	effect					1225:1230	So the effect	1218:1230	So the effect of SCA	1218:1237	So the effect of SCA was more pronounced than that of waxy rice starch on suppressing PhIP formation.
26887688	8	2	theme	creatinine	1184:1193	arg1	group					1175:1179	the amino group	1165:1179	the amino group of creatinine in the model reaction	1165:1215	Furthermore, the complex spatial structure of waxy rice starch disturbs the reaction of the glucosyl hydroxyl groups of glucose with the amino group of creatinine in the model reaction.
26887688	8	2	theme	creatinine	1184:1193	arg1	creatinine					1184:1193	creatinine	1184:1193	creatinine in the model reaction	1184:1215	Furthermore, the complex spatial structure of waxy rice starch disturbs the reaction of the glucosyl hydroxyl groups of glucose with the amino group of creatinine in the model reaction.
26887688	4	3	theme	chain	385:389	arg1	amylose					391:397	short chain amylose	379:397	short chain amylose	379:397	In this study, the effects of waxy rice starch and short chain amylose (SCA) from debranched waxy rice starch on the formation of PhIP in a model system were investigated and compared.
26887688	10	4	theme	SCA	1365:1367	arg1	mechanism					1331:1339	A possible mechanism	1320:1339	A possible mechanism of waxy rice starch and SCA for inhibiting PhIP formation in the model system	1320:1417	A possible mechanism of waxy rice starch and SCA for inhibiting PhIP formation in the model system is also proposed.
26887688	4	5	theme	debranched	410:419	arg1	starch					431:436	debranched waxy rice starch	410:436	debranched waxy rice starch	410:436	In this study, the effects of waxy rice starch and short chain amylose (SCA) from debranched waxy rice starch on the formation of PhIP in a model system were investigated and compared.
26887688	0	6	theme	model	138:142	arg1	system					144:149	a model system	136:149	a model system	136:149	Effects of waxy rice starch and short chain amylose (SCA) on the formation of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) in a model system.
26887688	8	7	theme	rice	1083:1086	arg1	starch					1088:1093	waxy rice starch	1078:1093	waxy rice starch	1078:1093	Furthermore, the complex spatial structure of waxy rice starch disturbs the reaction of the glucosyl hydroxyl groups of glucose with the amino group of creatinine in the model reaction.
26887688	10	8	theme	model	1406:1410	arg1	system					1412:1417	the model system	1402:1417	the model system	1402:1417	A possible mechanism of waxy rice starch and SCA for inhibiting PhIP formation in the model system is also proposed.
26887688	0	9	from	Effects	0:6	arg1	formation					65:73	the formation	61:73	the formation of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) in a model system	61:149	Effects of waxy rice starch and short chain amylose (SCA) on the formation of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) in a model system.
26887688	3	10	theme	important	292:300	arg1	material					302:309	an important material	289:309	an important material in cooking meat	289:325	Starch is often used as an important material in cooking meat.
26887688	3	10	theme	important	292:300	arg1	Starch					265:270	Starch	265:270	Starch	265:270	Starch is often used as an important material in cooking meat.
26887688	3	11	from	material	302:309	arg1	meat					322:325	cooking meat	314:325	cooking meat	314:325	Starch is often used as an important material in cooking meat.
26887688	4	12	theme	model	468:472	arg1	system					474:479	a model system	466:479	a model system	466:479	In this study, the effects of waxy rice starch and short chain amylose (SCA) from debranched waxy rice starch on the formation of PhIP in a model system were investigated and compared.
26887688	10	13	from	formation	1389:1397	arg1	system					1412:1417	the model system	1402:1417	the model system	1402:1417	A possible mechanism of waxy rice starch and SCA for inhibiting PhIP formation in the model system is also proposed.
26887688	4	14	theme	short	379:383	arg1	amylose					391:397	short chain amylose	379:397	short chain amylose	379:397	In this study, the effects of waxy rice starch and short chain amylose (SCA) from debranched waxy rice starch on the formation of PhIP in a model system were investigated and compared.
26887688	7	15	theme	condensation	956:967	arg1	formation					977:985	the aldol condensation product formation	946:985	the aldol condensation product formation	946:985	This reaction path could disturb the reaction of creatinine with phenylacetaldehyde, subsequently influence the aldol condensation product formation, and finally suppress the formation of PhIP.
26887688	7	16	with	reaction	875:882	arg1	phenylacetaldehyde					903:920	phenylacetaldehyde	903:920	phenylacetaldehyde	903:920	This reaction path could disturb the reaction of creatinine with phenylacetaldehyde, subsequently influence the aldol condensation product formation, and finally suppress the formation of PhIP.
26887688	2	17	theme	major	224:228	arg1	component					230:238	the major component	220:238	the major component of all our staple foods	220:262	It forms the major component of all our staple foods.
26887688	4	18	from	starch	431:436	arg1	effects					347:353	the effects	343:353	the effects of waxy rice starch and short chain amylose (SCA) from debranched waxy rice starch on the formation of PhIP in a model system	343:479	In this study, the effects of waxy rice starch and short chain amylose (SCA) from debranched waxy rice starch on the formation of PhIP in a model system were investigated and compared.
26887688	4	18	from	starch	431:436	arg1	amylose					391:397	short chain amylose	379:397	short chain amylose	379:397	In this study, the effects of waxy rice starch and short chain amylose (SCA) from debranched waxy rice starch on the formation of PhIP in a model system were investigated and compared.
26887688	4	18	from	starch	431:436	arg1	starch					368:373	waxy rice starch	358:373	waxy rice starch	358:373	In this study, the effects of waxy rice starch and short chain amylose (SCA) from debranched waxy rice starch on the formation of PhIP in a model system were investigated and compared.
26887688	4	18	from	starch	431:436	arg1	SCA					400:402	SCA	400:402	SCA	400:402	In this study, the effects of waxy rice starch and short chain amylose (SCA) from debranched waxy rice starch on the formation of PhIP in a model system were investigated and compared.
26887688	6	19	theme	conjugate	827:835	arg1	group					785:789	the amino group	775:789	the amino group of the creatinine formed N-glycosyl conjugate	775:835	This decrease may be attributed to the fact that the glucose residues of starch condense with the amino group of the creatinine formed N-glycosyl conjugate.
26887688	6	19	theme	conjugate	827:835	arg1	conjugate					827:835	the creatinine formed N-glycosyl conjugate	794:835	the creatinine formed N-glycosyl conjugate	794:835	This decrease may be attributed to the fact that the glucose residues of starch condense with the amino group of the creatinine formed N-glycosyl conjugate.
26887688	3	20	used	used	281:284	arg2	material					302:309	an important material	289:309	an important material in cooking meat	289:325	Starch is often used as an important material in cooking meat.
26887688	3	20	used	used	281:284	arg2	Starch					265:270	Starch	265:270	Starch	265:270	Starch is often used as an important material in cooking meat.
26887688	8	21	from	creatinine	1184:1193	arg1	reaction					1208:1215	the model reaction	1198:1215	the model reaction	1198:1215	Furthermore, the complex spatial structure of waxy rice starch disturbs the reaction of the glucosyl hydroxyl groups of glucose with the amino group of creatinine in the model reaction.
26887688	8	22	theme	model	1202:1206	arg1	reaction					1208:1215	the model reaction	1198:1215	the model reaction	1198:1215	Furthermore, the complex spatial structure of waxy rice starch disturbs the reaction of the glucosyl hydroxyl groups of glucose with the amino group of creatinine in the model reaction.
26887688	9	23	theme	rice	1277:1280	arg1	starch					1282:1287	waxy rice starch	1272:1287	waxy rice starch	1272:1287	So the effect of SCA was more pronounced than that of waxy rice starch on suppressing PhIP formation.
26887688	3	24	theme	cooking	314:320	arg1	meat					322:325	cooking meat	314:325	cooking meat	314:325	Starch is often used as an important material in cooking meat.
26887688	4	25	theme	amylose	391:397	arg1	effects					347:353	the effects	343:353	the effects of waxy rice starch and short chain amylose (SCA) from debranched waxy rice starch on the formation of PhIP in a model system	343:479	In this study, the effects of waxy rice starch and short chain amylose (SCA) from debranched waxy rice starch on the formation of PhIP in a model system were investigated and compared.
26887688	6	26	theme	glucose	734:740	arg1	starch					754:759	starch	754:759	starch	754:759	This decrease may be attributed to the fact that the glucose residues of starch condense with the amino group of the creatinine formed N-glycosyl conjugate.
26887688	6	26	theme	glucose	734:740	arg1	residues					742:749	the glucose residues	730:749	the glucose residues of starch	730:759	This decrease may be attributed to the fact that the glucose residues of starch condense with the amino group of the creatinine formed N-glycosyl conjugate.
26887688	2	27	theme	foods	258:262	arg1	component					230:238	the major component	220:238	the major component of all our staple foods	220:262	It forms the major component of all our staple foods.
26887688	5	28	theme	rice	558:561	arg1	starch					563:568	waxy rice starch	553:568	waxy rice starch	553:568	The results showed that the addition of waxy rice starch and SCA significantly decreased PhIP, and the effect of SCA was more pronounced than that of waxy rice starch.
26887688	5	29	theme	rice	668:671	arg1	starch					673:678	waxy rice starch	663:678	waxy rice starch	663:678	The results showed that the addition of waxy rice starch and SCA significantly decreased PhIP, and the effect of SCA was more pronounced than that of waxy rice starch.
26887688	6	30	theme	N-glycosyl	816:825	arg1	conjugate					827:835	the creatinine formed N-glycosyl conjugate	794:835	the creatinine formed N-glycosyl conjugate	794:835	This decrease may be attributed to the fact that the glucose residues of starch condense with the amino group of the creatinine formed N-glycosyl conjugate.
26887688	7	31	theme	aldol	950:954	arg1	formation					977:985	the aldol condensation product formation	946:985	the aldol condensation product formation	946:985	This reaction path could disturb the reaction of creatinine with phenylacetaldehyde, subsequently influence the aldol condensation product formation, and finally suppress the formation of PhIP.
26887688	8	32	from	group	1175:1179	arg1	reaction					1208:1215	the model reaction	1198:1215	the model reaction	1198:1215	Furthermore, the complex spatial structure of waxy rice starch disturbs the reaction of the glucosyl hydroxyl groups of glucose with the amino group of creatinine in the model reaction.
26887688	2	33	theme	staple	251:256	arg1	foods					258:262	all our staple foods	243:262	all our staple foods	243:262	It forms the major component of all our staple foods.
26887688	0	34	theme	rice	16:19	arg1	starch					21:26	waxy rice starch	11:26	waxy rice starch	11:26	Effects of waxy rice starch and short chain amylose (SCA) on the formation of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) in a model system.
26887688	6	35	theme	formed	809:814	arg1	conjugate					827:835	the creatinine formed N-glycosyl conjugate	794:835	the creatinine formed N-glycosyl conjugate	794:835	This decrease may be attributed to the fact that the glucose residues of starch condense with the amino group of the creatinine formed N-glycosyl conjugate.
26887688	5	36	theme	SCA	574:576	arg1	addition					541:548	the addition	537:548	the addition of waxy rice starch and SCA	537:576	The results showed that the addition of waxy rice starch and SCA significantly decreased PhIP, and the effect of SCA was more pronounced than that of waxy rice starch.
26887688	9	37	theme	waxy	1272:1275	arg1	starch					1282:1287	waxy rice starch	1272:1287	waxy rice starch	1272:1287	So the effect of SCA was more pronounced than that of waxy rice starch on suppressing PhIP formation.
26887688	8	38	theme	glucosyl	1124:1131	arg1	glucose					1152:1158	glucose	1152:1158	glucose	1152:1158	Furthermore, the complex spatial structure of waxy rice starch disturbs the reaction of the glucosyl hydroxyl groups of glucose with the amino group of creatinine in the model reaction.
26887688	8	38	theme	glucosyl	1124:1131	arg1	groups					1142:1147	the glucosyl hydroxyl groups	1120:1147	the glucosyl hydroxyl groups of glucose	1120:1158	Furthermore, the complex spatial structure of waxy rice starch disturbs the reaction of the glucosyl hydroxyl groups of glucose with the amino group of creatinine in the model reaction.
26887688	0	39	theme	waxy	11:14	arg1	starch					21:26	waxy rice starch	11:26	waxy rice starch	11:26	Effects of waxy rice starch and short chain amylose (SCA) on the formation of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) in a model system.
26887688	4	40	theme	PhIP	458:461	arg1	formation					445:453	the formation	441:453	the formation of PhIP in a model system	441:479	In this study, the effects of waxy rice starch and short chain amylose (SCA) from debranched waxy rice starch on the formation of PhIP in a model system were investigated and compared.
26887688	0	41	theme	2-amino-1-methyl-6-phenylimidazo[4,5-b	78:115	arg1	pyridine					117:124	2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine	78:124	2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP)	78:131	Effects of waxy rice starch and short chain amylose (SCA) on the formation of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) in a model system.
26887688	0	41	theme	2-amino-1-methyl-6-phenylimidazo[4,5-b	78:115	arg1	PhIP					127:130	PhIP	127:130	PhIP	127:130	Effects of waxy rice starch and short chain amylose (SCA) on the formation of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) in a model system.
26887688	4	42	theme	rice	363:366	arg1	starch					368:373	waxy rice starch	358:373	waxy rice starch	358:373	In this study, the effects of waxy rice starch and short chain amylose (SCA) from debranched waxy rice starch on the formation of PhIP in a model system were investigated and compared.
26887688	4	42	theme	rice	363:366	arg1	SCA					400:402	SCA	400:402	SCA	400:402	In this study, the effects of waxy rice starch and short chain amylose (SCA) from debranched waxy rice starch on the formation of PhIP in a model system were investigated and compared.
26887688	9	43	theme	PhIP	1304:1307	arg1	formation					1309:1317	PhIP formation	1304:1317	PhIP formation	1304:1317	So the effect of SCA was more pronounced than that of waxy rice starch on suppressing PhIP formation.
26887688	8	44	theme	waxy	1078:1081	arg1	starch					1088:1093	waxy rice starch	1078:1093	waxy rice starch	1078:1093	Furthermore, the complex spatial structure of waxy rice starch disturbs the reaction of the glucosyl hydroxyl groups of glucose with the amino group of creatinine in the model reaction.
26887688	10	45	theme	possible	1322:1329	arg1	mechanism					1331:1339	A possible mechanism	1320:1339	A possible mechanism of waxy rice starch and SCA for inhibiting PhIP formation in the model system	1320:1417	A possible mechanism of waxy rice starch and SCA for inhibiting PhIP formation in the model system is also proposed.
26887688	4	46	theme	waxy	358:361	arg1	starch					368:373	waxy rice starch	358:373	waxy rice starch	358:373	In this study, the effects of waxy rice starch and short chain amylose (SCA) from debranched waxy rice starch on the formation of PhIP in a model system were investigated and compared.
26887688	4	46	theme	waxy	358:361	arg1	SCA					400:402	SCA	400:402	SCA	400:402	In this study, the effects of waxy rice starch and short chain amylose (SCA) from debranched waxy rice starch on the formation of PhIP in a model system were investigated and compared.
26887688	8	47	theme	complex	1049:1055	arg1	structure					1065:1073	the complex spatial structure	1045:1073	the complex spatial structure of waxy rice starch	1045:1093	Furthermore, the complex spatial structure of waxy rice starch disturbs the reaction of the glucosyl hydroxyl groups of glucose with the amino group of creatinine in the model reaction.
26887688	1	48	theme	glucose	164:170	arg1	Starch					152:157	Starch	152:157	Starch	152:157	Starch is a glucose polymer of vast importance to mankind.
26887688	1	48	theme	glucose	164:170	arg1	polymer					172:178	a glucose polymer	162:178	a glucose polymer of vast importance to mankind	162:208	Starch is a glucose polymer of vast importance to mankind.
26887688	0	49	theme	starch	21:26	arg1	Effects					0:6	Effects	0:6	Effects of waxy rice starch and short chain amylose (SCA) on the formation of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) in a model system.	0:150	Effects of waxy rice starch and short chain amylose (SCA) on the formation of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) in a model system.
26887688	4	50	theme	rice	426:429	arg1	starch					431:436	debranched waxy rice starch	410:436	debranched waxy rice starch	410:436	In this study, the effects of waxy rice starch and short chain amylose (SCA) from debranched waxy rice starch on the formation of PhIP in a model system were investigated and compared.
26887688	0	51	theme	pyridine	117:124	arg1	formation					65:73	the formation	61:73	the formation of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) in a model system	61:149	Effects of waxy rice starch and short chain amylose (SCA) on the formation of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) in a model system.
26887688	8	52	theme	groups	1142:1147	arg1	reaction					1108:1115	the reaction	1104:1115	the reaction of the glucosyl hydroxyl groups of glucose with the amino group of creatinine in the model reaction	1104:1215	Furthermore, the complex spatial structure of waxy rice starch disturbs the reaction of the glucosyl hydroxyl groups of glucose with the amino group of creatinine in the model reaction.
26887688	4	53	from	formation	445:453	arg1	system					474:479	a model system	466:479	a model system	466:479	In this study, the effects of waxy rice starch and short chain amylose (SCA) from debranched waxy rice starch on the formation of PhIP in a model system were investigated and compared.
26887688	8	54	theme	hydroxyl	1133:1140	arg1	glucose					1152:1158	glucose	1152:1158	glucose	1152:1158	Furthermore, the complex spatial structure of waxy rice starch disturbs the reaction of the glucosyl hydroxyl groups of glucose with the amino group of creatinine in the model reaction.
26887688	8	54	theme	hydroxyl	1133:1140	arg1	groups					1142:1147	the glucosyl hydroxyl groups	1120:1147	the glucosyl hydroxyl groups of glucose	1120:1158	Furthermore, the complex spatial structure of waxy rice starch disturbs the reaction of the glucosyl hydroxyl groups of glucose with the amino group of creatinine in the model reaction.
26887688	0	55	theme	chain	38:42	arg1	SCA					53:55	SCA	53:55	SCA	53:55	Effects of waxy rice starch and short chain amylose (SCA) on the formation of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) in a model system.
26887688	0	55	theme	chain	38:42	arg1	amylose					44:50	short chain amylose	32:50	short chain amylose (SCA)	32:56	Effects of waxy rice starch and short chain amylose (SCA) on the formation of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) in a model system.
26887688	4	56	theme	waxy	421:424	arg1	starch					431:436	debranched waxy rice starch	410:436	debranched waxy rice starch	410:436	In this study, the effects of waxy rice starch and short chain amylose (SCA) from debranched waxy rice starch on the formation of PhIP in a model system were investigated and compared.
26887688	5	57	theme	waxy	553:556	arg1	starch					563:568	waxy rice starch	553:568	waxy rice starch	553:568	The results showed that the addition of waxy rice starch and SCA significantly decreased PhIP, and the effect of SCA was more pronounced than that of waxy rice starch.
26887688	4	58	theme	starch	368:373	arg1	effects					347:353	the effects	343:353	the effects of waxy rice starch and short chain amylose (SCA) from debranched waxy rice starch on the formation of PhIP in a model system	343:479	In this study, the effects of waxy rice starch and short chain amylose (SCA) from debranched waxy rice starch on the formation of PhIP in a model system were investigated and compared.
26887688	10	59	theme	rice	1349:1352	arg1	starch					1354:1359	waxy rice starch	1344:1359	waxy rice starch	1344:1359	A possible mechanism of waxy rice starch and SCA for inhibiting PhIP formation in the model system is also proposed.
26887688	8	60	theme	glucose	1152:1158	arg1	glucose					1152:1158	glucose	1152:1158	glucose	1152:1158	Furthermore, the complex spatial structure of waxy rice starch disturbs the reaction of the glucosyl hydroxyl groups of glucose with the amino group of creatinine in the model reaction.
26887688	8	60	theme	glucose	1152:1158	arg1	groups					1142:1147	the glucosyl hydroxyl groups	1120:1147	the glucosyl hydroxyl groups of glucose	1120:1158	Furthermore, the complex spatial structure of waxy rice starch disturbs the reaction of the glucosyl hydroxyl groups of glucose with the amino group of creatinine in the model reaction.
26887688	0	61	theme	short	32:36	arg1	SCA					53:55	SCA	53:55	SCA	53:55	Effects of waxy rice starch and short chain amylose (SCA) on the formation of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) in a model system.
26887688	0	61	theme	short	32:36	arg1	amylose					44:50	short chain amylose	32:50	short chain amylose (SCA)	32:56	Effects of waxy rice starch and short chain amylose (SCA) on the formation of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) in a model system.
26887688	6	62	theme	amino	779:783	arg1	group					785:789	the amino group	775:789	the amino group of the creatinine formed N-glycosyl conjugate	775:835	This decrease may be attributed to the fact that the glucose residues of starch condense with the amino group of the creatinine formed N-glycosyl conjugate.
26887688	6	62	theme	amino	779:783	arg1	conjugate					827:835	the creatinine formed N-glycosyl conjugate	794:835	the creatinine formed N-glycosyl conjugate	794:835	This decrease may be attributed to the fact that the glucose residues of starch condense with the amino group of the creatinine formed N-glycosyl conjugate.
26887688	4	63	from	effects	347:353	arg1	starch					431:436	debranched waxy rice starch	410:436	debranched waxy rice starch	410:436	In this study, the effects of waxy rice starch and short chain amylose (SCA) from debranched waxy rice starch on the formation of PhIP in a model system were investigated and compared.
26887688	4	63	from	effects	347:353	arg1	formation					445:453	the formation	441:453	the formation of PhIP in a model system	441:479	In this study, the effects of waxy rice starch and short chain amylose (SCA) from debranched waxy rice starch on the formation of PhIP in a model system were investigated and compared.
26887688	6	64	theme	starch	754:759	arg1	starch					754:759	starch	754:759	starch	754:759	This decrease may be attributed to the fact that the glucose residues of starch condense with the amino group of the creatinine formed N-glycosyl conjugate.
26887688	6	64	theme	starch	754:759	arg1	residues					742:749	the glucose residues	730:749	the glucose residues of starch	730:759	This decrease may be attributed to the fact that the glucose residues of starch condense with the amino group of the creatinine formed N-glycosyl conjugate.
26887688	7	65	theme	product	969:975	arg1	formation					977:985	the aldol condensation product formation	946:985	the aldol condensation product formation	946:985	This reaction path could disturb the reaction of creatinine with phenylacetaldehyde, subsequently influence the aldol condensation product formation, and finally suppress the formation of PhIP.
26887688	8	66	from	reaction	1208:1215	arg1	group					1175:1179	the amino group	1165:1179	the amino group of creatinine in the model reaction	1165:1215	Furthermore, the complex spatial structure of waxy rice starch disturbs the reaction of the glucosyl hydroxyl groups of glucose with the amino group of creatinine in the model reaction.
26887688	8	66	from	reaction	1208:1215	arg1	creatinine					1184:1193	creatinine	1184:1193	creatinine in the model reaction	1184:1215	Furthermore, the complex spatial structure of waxy rice starch disturbs the reaction of the glucosyl hydroxyl groups of glucose with the amino group of creatinine in the model reaction.
26887688	7	67	theme	PhIP	1026:1029	arg1	formation					1013:1021	the formation	1009:1021	the formation of PhIP	1009:1029	This reaction path could disturb the reaction of creatinine with phenylacetaldehyde, subsequently influence the aldol condensation product formation, and finally suppress the formation of PhIP.
26887688	5	68	theme	starch	563:568	arg1	addition					541:548	the addition	537:548	the addition of waxy rice starch and SCA	537:576	The results showed that the addition of waxy rice starch and SCA significantly decreased PhIP, and the effect of SCA was more pronounced than that of waxy rice starch.
26887688	0	69	from	formation	65:73	arg1	system					144:149	a model system	136:149	a model system	136:149	Effects of waxy rice starch and short chain amylose (SCA) on the formation of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) in a model system.
26887688	5	70	theme	SCA	626:628	arg1	pronounced					639:648	pronounced	639:648	pronounced	639:648	The results showed that the addition of waxy rice starch and SCA significantly decreased PhIP, and the effect of SCA was more pronounced than that of waxy rice starch.
26887688	5	70	theme	SCA	626:628	arg1	effect					616:621	the effect	612:621	the effect of SCA	612:628	The results showed that the addition of waxy rice starch and SCA significantly decreased PhIP, and the effect of SCA was more pronounced than that of waxy rice starch.
26887688	10	71	theme	waxy	1344:1347	arg1	starch					1354:1359	waxy rice starch	1344:1359	waxy rice starch	1344:1359	A possible mechanism of waxy rice starch and SCA for inhibiting PhIP formation in the model system is also proposed.
26887688	8	72	theme	amino	1169:1173	arg1	group					1175:1179	the amino group	1165:1179	the amino group of creatinine in the model reaction	1165:1215	Furthermore, the complex spatial structure of waxy rice starch disturbs the reaction of the glucosyl hydroxyl groups of glucose with the amino group of creatinine in the model reaction.
26887688	8	72	theme	amino	1169:1173	arg1	creatinine					1184:1193	creatinine	1184:1193	creatinine in the model reaction	1184:1215	Furthermore, the complex spatial structure of waxy rice starch disturbs the reaction of the glucosyl hydroxyl groups of glucose with the amino group of creatinine in the model reaction.
26887688	8	73	theme	starch	1088:1093	arg1	structure					1065:1073	the complex spatial structure	1045:1073	the complex spatial structure of waxy rice starch	1045:1093	Furthermore, the complex spatial structure of waxy rice starch disturbs the reaction of the glucosyl hydroxyl groups of glucose with the amino group of creatinine in the model reaction.
26887688	1	74	theme	vast	183:186	arg1	importance					188:197	vast importance	183:197	vast importance	183:197	Starch is a glucose polymer of vast importance to mankind.
26887688	10	75	theme	PhIP	1384:1387	arg1	formation					1389:1397	PhIP formation	1384:1397	PhIP formation in the model system	1384:1417	A possible mechanism of waxy rice starch and SCA for inhibiting PhIP formation in the model system is also proposed.
26887688	6	76	dep	formed	809:814	arg1	creatinine					798:807	creatinine	798:807	creatinine	798:807	This decrease may be attributed to the fact that the glucose residues of starch condense with the amino group of the creatinine formed N-glycosyl conjugate.
26887688	7	77	theme	creatinine	887:896	arg1	reaction					875:882	the reaction	871:882	the reaction of creatinine with phenylacetaldehyde	871:920	This reaction path could disturb the reaction of creatinine with phenylacetaldehyde, subsequently influence the aldol condensation product formation, and finally suppress the formation of PhIP.
26887688	7	78	theme	reaction	843:850	arg1	path					852:855	This reaction path	838:855	This reaction path	838:855	This reaction path could disturb the reaction of creatinine with phenylacetaldehyde, subsequently influence the aldol condensation product formation, and finally suppress the formation of PhIP.
26887688	10	79	theme	starch	1354:1359	arg1	mechanism					1331:1339	A possible mechanism	1320:1339	A possible mechanism of waxy rice starch and SCA for inhibiting PhIP formation in the model system	1320:1417	A possible mechanism of waxy rice starch and SCA for inhibiting PhIP formation in the model system is also proposed.
26887688	1	80	theme	importance	188:197	arg1	Starch					152:157	Starch	152:157	Starch	152:157	Starch is a glucose polymer of vast importance to mankind.
26887688	1	80	theme	importance	188:197	arg1	polymer					172:178	a glucose polymer	162:178	a glucose polymer of vast importance to mankind	162:208	Starch is a glucose polymer of vast importance to mankind.
26887688	0	81	theme	amylose	44:50	arg1	Effects					0:6	Effects	0:6	Effects of waxy rice starch and short chain amylose (SCA) on the formation of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) in a model system.	0:150	Effects of waxy rice starch and short chain amylose (SCA) on the formation of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) in a model system.
26887688	8	82	theme	spatial	1057:1063	arg1	structure					1065:1073	the complex spatial structure	1045:1073	the complex spatial structure of waxy rice starch	1045:1093	Furthermore, the complex spatial structure of waxy rice starch disturbs the reaction of the glucosyl hydroxyl groups of glucose with the amino group of creatinine in the model reaction.
26887688	5	83	theme	waxy	663:666	arg1	starch					673:678	waxy rice starch	663:678	waxy rice starch	663:678	The results showed that the addition of waxy rice starch and SCA significantly decreased PhIP, and the effect of SCA was more pronounced than that of waxy rice starch.
29558056	6	0	theme	mycelium	843:850	arg1	powder					819:824	Dry powder	815:824	Dry powder of the submerged mycelium	815:850	Dry powder of the submerged mycelium, water extract of the mycelium and total fraction of the water-soluble polysaccharides demonstrated the antitumor activity against murine lymphocytic leukemia P 388 in vivo.
29558056	6	0	theme	mycelium	843:850	arg1	extract					859:865	water extract	853:865	water extract of the mycelium	853:881	Dry powder of the submerged mycelium, water extract of the mycelium and total fraction of the water-soluble polysaccharides demonstrated the antitumor activity against murine lymphocytic leukemia P 388 in vivo.
29558056	6	0	theme	mycelium	843:850	arg1	fraction					893:900	total fraction	887:900	total fraction of the water-soluble polysaccharides	887:937	Dry powder of the submerged mycelium, water extract of the mycelium and total fraction of the water-soluble polysaccharides demonstrated the antitumor activity against murine lymphocytic leukemia P 388 in vivo.
29558056	1	1	theme	optimized	287:295	arg1	conditions					297:306	optimized conditions	287:306	optimized conditions	287:306	The aim of the study was to evaluate antitumor and antioxidant properties of water-soluble polysaccharides from submerged myceliumn of Flammulina velutipes grown under optimized conditions.
29558056	10	2	theme	Grifolafrondosa	1549:1563	arg1	mycelium					1537:1544	the sub- merged mycelium	1521:1544	the sub- merged mycelium of Grifolafrondosa	1521:1563	The antioxidant capacity (AOC) of the water-soluble fraction from F.velutipes was higher than that of the water-soluble polysaccharides from the sub- merged mycelium of Grifolafrondosa, but inferior to the AOC of the water-soluble polysaccharides from the submerged mycelium of Ganoderma luciduma.
29558056	10	3	from	AOC	1586:1588	arg1	mycelium					1646:1653	the submerged mycelium	1632:1653	the submerged mycelium of Ganoderma luciduma	1632:1675	The antioxidant capacity (AOC) of the water-soluble fraction from F.velutipes was higher than that of the water-soluble polysaccharides from the sub- merged mycelium of Grifolafrondosa, but inferior to the AOC of the water-soluble polysaccharides from the submerged mycelium of Ganoderma luciduma.
29558056	8	4	theme	tumor	1182:1186	arg1	inhibition					1195:1204	The maximum tumor growth inhibition	1170:1204	The maximum tumor growth inhibition by the water-soluble polysaccharides	1170:1241	The maximum tumor growth inhibition by the water-soluble polysaccharides amounted to 94%.
29558056	10	5	theme	antioxidant	1384:1394	arg1	AOC					1406:1408	AOC	1406:1408	AOC	1406:1408	The antioxidant capacity (AOC) of the water-soluble fraction from F.velutipes was higher than that of the water-soluble polysaccharides from the sub- merged mycelium of Grifolafrondosa, but inferior to the AOC of the water-soluble polysaccharides from the submerged mycelium of Ganoderma luciduma.
29558056	10	5	theme	antioxidant	1384:1394	arg1	capacity					1396:1403	The antioxidant capacity	1380:1403	The antioxidant capacity (AOC) of the water-soluble fraction from F.velutipes	1380:1456	The antioxidant capacity (AOC) of the water-soluble fraction from F.velutipes was higher than that of the water-soluble polysaccharides from the sub- merged mycelium of Grifolafrondosa, but inferior to the AOC of the water-soluble polysaccharides from the submerged mycelium of Ganoderma luciduma.
29558056	10	5	theme	antioxidant	1384:1394	arg1	higher					1462:1467	higher	1462:1467	higher	1462:1467	The antioxidant capacity (AOC) of the water-soluble fraction from F.velutipes was higher than that of the water-soluble polysaccharides from the sub- merged mycelium of Grifolafrondosa, but inferior to the AOC of the water-soluble polysaccharides from the submerged mycelium of Ganoderma luciduma.
29558056	3	6	theme	polysaccharides	571:585	arg1	%					592:592	31.6%	588:592	31.6% of proteins	588:604	The submerged mycelium of F.velutipes strain Fv-1 contained 14.8% of a water-soluble polysaccharides, 31.6% of proteins, 2.5% of total lipids, vitamins B (B1, B5, B6).
29558056	3	6	theme	polysaccharides	571:585	arg1	lipids					621:626	total lipids	615:626	total lipids	615:626	The submerged mycelium of F.velutipes strain Fv-1 contained 14.8% of a water-soluble polysaccharides, 31.6% of proteins, 2.5% of total lipids, vitamins B (B1, B5, B6).
29558056	3	6	theme	polysaccharides	571:585	arg1	proteins					597:604	proteins	597:604	proteins	597:604	The submerged mycelium of F.velutipes strain Fv-1 contained 14.8% of a water-soluble polysaccharides, 31.6% of proteins, 2.5% of total lipids, vitamins B (B1, B5, B6).
29558056	3	6	theme	polysaccharides	571:585	arg1	polysaccharides					571:585	a water-soluble polysaccharides	555:585	a water-soluble polysaccharides	555:585	The submerged mycelium of F.velutipes strain Fv-1 contained 14.8% of a water-soluble polysaccharides, 31.6% of proteins, 2.5% of total lipids, vitamins B (B1, B5, B6).
29558056	3	6	theme	polysaccharides	571:585	arg1	%					610:610	2.5%	607:610	2.5% of total lipids	607:626	The submerged mycelium of F.velutipes strain Fv-1 contained 14.8% of a water-soluble polysaccharides, 31.6% of proteins, 2.5% of total lipids, vitamins B (B1, B5, B6).
29558056	3	6	theme	polysaccharides	571:585	arg1	B					638:638	vitamins B	629:638	vitamins B (B1, B5, B6)	629:651	The submerged mycelium of F.velutipes strain Fv-1 contained 14.8% of a water-soluble polysaccharides, 31.6% of proteins, 2.5% of total lipids, vitamins B (B1, B5, B6).
29558056	3	6	theme	polysaccharides	571:585	arg1	%					550:550	14.8%	546:550	14.8% of a water-soluble polysaccharides	546:585	The submerged mycelium of F.velutipes strain Fv-1 contained 14.8% of a water-soluble polysaccharides, 31.6% of proteins, 2.5% of total lipids, vitamins B (B1, B5, B6).
29558056	1	7	theme	water-soluble	196:208	arg1	polysaccharides					210:224	water-soluble polysaccharides	196:224	water-soluble polysaccharides from submerged myceliumn of Flammulina velutipes grown under optimized conditions	196:306	The aim of the study was to evaluate antitumor and antioxidant properties of water-soluble polysaccharides from submerged myceliumn of Flammulina velutipes grown under optimized conditions.
29558056	6	8	theme	total	887:891	arg1	fraction					893:900	total fraction	887:900	total fraction of the water-soluble polysaccharides	887:937	Dry powder of the submerged mycelium, water extract of the mycelium and total fraction of the water-soluble polysaccharides demonstrated the antitumor activity against murine lymphocytic leukemia P 388 in vivo.
29558056	4	9	contain	contained	674:682	arg2	glucose					684:690	glucose	684:690	glucose	684:690	The polysaccharides contained glucose, galactose, fucose, mannose, xylose, rhamnose.
29558056	4	9	contain	contained	674:682	arg1	polysaccharides					658:672	The polysaccharides	654:672	The polysaccharides	654:672	The polysaccharides contained glucose, galactose, fucose, mannose, xylose, rhamnose.
29558056	4	9	contain	contained	674:682	arg2	galactose					693:701	galactose	693:701	galactose	693:701	The polysaccharides contained glucose, galactose, fucose, mannose, xylose, rhamnose.
29558056	4	9	contain	contained	674:682	arg2	xylose					721:726	xylose	721:726	xylose	721:726	The polysaccharides contained glucose, galactose, fucose, mannose, xylose, rhamnose.
29558056	4	9	contain	contained	674:682	arg2	fucose					704:709	fucose	704:709	fucose	704:709	The polysaccharides contained glucose, galactose, fucose, mannose, xylose, rhamnose.
29558056	4	9	contain	contained	674:682	arg2	rhamnose					729:736	rhamnose	729:736	rhamnose	729:736	The polysaccharides contained glucose, galactose, fucose, mannose, xylose, rhamnose.
29558056	4	9	contain	contained	674:682	arg2	mannose					712:718	mannose	712:718	mannose	712:718	The polysaccharides contained glucose, galactose, fucose, mannose, xylose, rhamnose.
29558056	2	10	theme	nutrient	333:340	arg1	composition					349:359	the nutrient medium composition	329:359	the nutrient medium composition	329:359	The optimization of the nutrient medium composition allowed to increase the biomass yield by more than 2 times (up to 35 g/l) and to reduce the time of the cultivation process.
29558056	6	11	theme	mycelium	874:881	arg1	powder					819:824	Dry powder	815:824	Dry powder of the submerged mycelium	815:850	Dry powder of the submerged mycelium, water extract of the mycelium and total fraction of the water-soluble polysaccharides demonstrated the antitumor activity against murine lymphocytic leukemia P 388 in vivo.
29558056	6	11	theme	mycelium	874:881	arg1	extract					859:865	water extract	853:865	water extract of the mycelium	853:881	Dry powder of the submerged mycelium, water extract of the mycelium and total fraction of the water-soluble polysaccharides demonstrated the antitumor activity against murine lymphocytic leukemia P 388 in vivo.
29558056	6	11	theme	mycelium	874:881	arg1	fraction					893:900	total fraction	887:900	total fraction of the water-soluble polysaccharides	887:937	Dry powder of the submerged mycelium, water extract of the mycelium and total fraction of the water-soluble polysaccharides demonstrated the antitumor activity against murine lymphocytic leukemia P 388 in vivo.
29558056	3	12	dep	B	638:638	arg1	B6					649:650	B6	649:650	B6	649:650	The submerged mycelium of F.velutipes strain Fv-1 contained 14.8% of a water-soluble polysaccharides, 31.6% of proteins, 2.5% of total lipids, vitamins B (B1, B5, B6).
29558056	3	12	dep	B	638:638	arg1	B1					641:642	B1	641:642	B1	641:642	The submerged mycelium of F.velutipes strain Fv-1 contained 14.8% of a water-soluble polysaccharides, 31.6% of proteins, 2.5% of total lipids, vitamins B (B1, B5, B6).
29558056	5	13	theme	amino	780:784	arg1	acids					786:790	the essential amino acids	766:790	the essential amino acids	766:790	The proteins contained all the essential amino acids except for tryptophan.
29558056	6	14	theme	water	853:857	arg1	extract					859:865	water extract	853:865	water extract of the mycelium	853:881	Dry powder of the submerged mycelium, water extract of the mycelium and total fraction of the water-soluble polysaccharides demonstrated the antitumor activity against murine lymphocytic leukemia P 388 in vivo.
29558056	6	15	theme	antitumor	956:964	arg1	activity					966:973	the antitumor activity	952:973	the antitumor activity against murine lymphocytic leukemia P 388	952:1015	Dry powder of the submerged mycelium, water extract of the mycelium and total fraction of the water-soluble polysaccharides demonstrated the antitumor activity against murine lymphocytic leukemia P 388 in vivo.
29558056	0	16	theme	Submerged	75:83	arg1	Mycelium					85:92	Submerged Mycelium	75:92	Submerged Mycelium of Flammulina velutipes	75:116	Antitumor and Antioxidant Properties of Water-Soluble Polysaccharides from Submerged Mycelium of Flammulina velutipes.
29558056	9	17	from	Fv-1	1340:1343	arg1	polysaccharides					1300:1314	the water-soluble polysaccharides	1282:1314	the water-soluble polysaccharides from F.velutipes strain Fv-1	1282:1343	The total fraction of the water-soluble polysaccharides from F.velutipes strain Fv-1 demonstrated antioxidant activity.
29558056	9	17	from	Fv-1	1340:1343	arg1	fraction					1270:1277	The total fraction	1260:1277	The total fraction of the water-soluble polysaccharides from F.velutipes strain Fv-1	1260:1343	The total fraction of the water-soluble polysaccharides from F.velutipes strain Fv-1 demonstrated antioxidant activity.
29558056	6	18	theme	water-soluble	909:921	arg1	polysaccharides					923:937	the water-soluble polysaccharides	905:937	the water-soluble polysaccharides	905:937	Dry powder of the submerged mycelium, water extract of the mycelium and total fraction of the water-soluble polysaccharides demonstrated the antitumor activity against murine lymphocytic leukemia P 388 in vivo.
29558056	0	19	from	Antitumor	0:8	arg1	Mycelium					85:92	Submerged Mycelium	75:92	Submerged Mycelium of Flammulina velutipes	75:116	Antitumor and Antioxidant Properties of Water-Soluble Polysaccharides from Submerged Mycelium of Flammulina velutipes.
29558056	0	20	theme	velutipes	108:116	arg1	Mycelium					85:92	Submerged Mycelium	75:92	Submerged Mycelium of Flammulina velutipes	75:116	Antitumor and Antioxidant Properties of Water-Soluble Polysaccharides from Submerged Mycelium of Flammulina velutipes.
29558056	10	21	theme	submerged	1636:1644	arg1	mycelium					1646:1653	the submerged mycelium	1632:1653	the submerged mycelium of Ganoderma luciduma	1632:1675	The antioxidant capacity (AOC) of the water-soluble fraction from F.velutipes was higher than that of the water-soluble polysaccharides from the sub- merged mycelium of Grifolafrondosa, but inferior to the AOC of the water-soluble polysaccharides from the submerged mycelium of Ganoderma luciduma.
29558056	9	22	theme	total	1264:1268	arg1	fraction					1270:1277	The total fraction	1260:1277	The total fraction of the water-soluble polysaccharides from F.velutipes strain Fv-1	1260:1343	The total fraction of the water-soluble polysaccharides from F.velutipes strain Fv-1 demonstrated antioxidant activity.
29558056	10	23	theme	fraction	1432:1439	arg1	AOC					1406:1408	AOC	1406:1408	AOC	1406:1408	The antioxidant capacity (AOC) of the water-soluble fraction from F.velutipes was higher than that of the water-soluble polysaccharides from the sub- merged mycelium of Grifolafrondosa, but inferior to the AOC of the water-soluble polysaccharides from the submerged mycelium of Ganoderma luciduma.
29558056	10	23	theme	fraction	1432:1439	arg1	capacity					1396:1403	The antioxidant capacity	1380:1403	The antioxidant capacity (AOC) of the water-soluble fraction from F.velutipes	1380:1456	The antioxidant capacity (AOC) of the water-soluble fraction from F.velutipes was higher than that of the water-soluble polysaccharides from the sub- merged mycelium of Grifolafrondosa, but inferior to the AOC of the water-soluble polysaccharides from the submerged mycelium of Ganoderma luciduma.
29558056	10	23	theme	fraction	1432:1439	arg1	higher					1462:1467	higher	1462:1467	higher	1462:1467	The antioxidant capacity (AOC) of the water-soluble fraction from F.velutipes was higher than that of the water-soluble polysaccharides from the sub- merged mycelium of Grifolafrondosa, but inferior to the AOC of the water-soluble polysaccharides from the submerged mycelium of Ganoderma luciduma.
29558056	6	24	theme	P	1011:1011	arg1	388					1013:1015	murine lymphocytic leukemia P 388	983:1015	murine lymphocytic leukemia P 388	983:1015	Dry powder of the submerged mycelium, water extract of the mycelium and total fraction of the water-soluble polysaccharides demonstrated the antitumor activity against murine lymphocytic leukemia P 388 in vivo.
29558056	10	25	theme	luciduma	1668:1675	arg1	mycelium					1646:1653	the submerged mycelium	1632:1653	the submerged mycelium of Ganoderma luciduma	1632:1675	The antioxidant capacity (AOC) of the water-soluble fraction from F.velutipes was higher than that of the water-soluble polysaccharides from the sub- merged mycelium of Grifolafrondosa, but inferior to the AOC of the water-soluble polysaccharides from the submerged mycelium of Ganoderma luciduma.
29558056	0	26	theme	Antioxidant	14:24	arg1	Properties					26:35	Antioxidant Properties	14:35	Antioxidant Properties	14:35	Antitumor and Antioxidant Properties of Water-Soluble Polysaccharides from Submerged Mycelium of Flammulina velutipes.
29558056	9	27	theme	water-soluble	1286:1298	arg1	polysaccharides					1300:1314	the water-soluble polysaccharides	1282:1314	the water-soluble polysaccharides from F.velutipes strain Fv-1	1282:1343	The total fraction of the water-soluble polysaccharides from F.velutipes strain Fv-1 demonstrated antioxidant activity.
29558056	6	28	theme	lymphocytic	990:1000	arg1	leukemia					1002:1009	murine lymphocytic leukemia	983:1009	murine lymphocytic leukemia P 388	983:1015	Dry powder of the submerged mycelium, water extract of the mycelium and total fraction of the water-soluble polysaccharides demonstrated the antitumor activity against murine lymphocytic leukemia P 388 in vivo.
29558056	2	29	theme	cultivation	465:475	arg1	process					477:483	the cultivation process	461:483	the cultivation process	461:483	The optimization of the nutrient medium composition allowed to increase the biomass yield by more than 2 times (up to 35 g/l) and to reduce the time of the cultivation process.
29558056	2	30	dep	35	427:428	arg1	to					424:425	to	424:425	to	424:425	The optimization of the nutrient medium composition allowed to increase the biomass yield by more than 2 times (up to 35 g/l) and to reduce the time of the cultivation process.
29558056	3	31	theme	total	615:619	arg1	lipids					621:626	total lipids	615:626	total lipids	615:626	The submerged mycelium of F.velutipes strain Fv-1 contained 14.8% of a water-soluble polysaccharides, 31.6% of proteins, 2.5% of total lipids, vitamins B (B1, B5, B6).
29558056	2	32	theme	biomass	385:391	arg1	yield					393:397	the biomass yield	381:397	the biomass yield by more than 2 times (up to 35 g/l)	381:433	The optimization of the nutrient medium composition allowed to increase the biomass yield by more than 2 times (up to 35 g/l) and to reduce the time of the cultivation process.
29558056	0	33	theme	Polysaccharides	54:68	arg1	Antitumor					0:8	Antitumor	0:8	Antitumor	0:8	Antitumor and Antioxidant Properties of Water-Soluble Polysaccharides from Submerged Mycelium of Flammulina velutipes.
29558056	0	33	theme	Polysaccharides	54:68	arg1	Properties					26:35	Antioxidant Properties	14:35	Antioxidant Properties	14:35	Antitumor and Antioxidant Properties of Water-Soluble Polysaccharides from Submerged Mycelium of Flammulina velutipes.
29558056	9	34	theme	strain	1333:1338	arg1	Fv-1					1340:1343	F.velutipes strain Fv-1	1321:1343	F.velutipes strain Fv-1	1321:1343	The total fraction of the water-soluble polysaccharides from F.velutipes strain Fv-1 demonstrated antioxidant activity.
29558056	10	35	theme	merged	1530:1535	arg1	mycelium					1537:1544	the sub- merged mycelium	1521:1544	the sub- merged mycelium of Grifolafrondosa	1521:1563	The antioxidant capacity (AOC) of the water-soluble fraction from F.velutipes was higher than that of the water-soluble polysaccharides from the sub- merged mycelium of Grifolafrondosa, but inferior to the AOC of the water-soluble polysaccharides from the submerged mycelium of Ganoderma luciduma.
29558056	3	36	theme	F.velutipes	512:522	arg1	Fv-1					531:534	F.velutipes strain Fv-1	512:534	F.velutipes strain Fv-1	512:534	The submerged mycelium of F.velutipes strain Fv-1 contained 14.8% of a water-soluble polysaccharides, 31.6% of proteins, 2.5% of total lipids, vitamins B (B1, B5, B6).
29558056	1	37	theme	velutipes	265:273	arg1	myceliumn					241:249	submerged myceliumn	231:249	submerged myceliumn of Flammulina velutipes grown under optimized conditions	231:306	The aim of the study was to evaluate antitumor and antioxidant properties of water-soluble polysaccharides from submerged myceliumn of Flammulina velutipes grown under optimized conditions.
29558056	7	38	theme	growth	1151:1156	arg1	inhibition					1158:1167	the tumor growth inhibition	1141:1167	the tumor growth inhibition	1141:1167	The antitumor activity of the substances was mainly due to the polysaccharides, since their purification increased the tumor growth inhibition.
29558056	7	39	theme	substances	1056:1065	arg1	activity					1040:1047	The antitumor activity	1026:1047	The antitumor activity of the substances	1026:1065	The antitumor activity of the substances was mainly due to the polysaccharides, since their purification increased the tumor growth inhibition.
29558056	7	39	theme	substances	1056:1065	arg1	due					1078:1080	due	1078:1080	due	1078:1080	The antitumor activity of the substances was mainly due to the polysaccharides, since their purification increased the tumor growth inhibition.
29558056	6	40	theme	submerged	833:841	arg1	mycelium					843:850	the submerged mycelium	829:850	the submerged mycelium	829:850	Dry powder of the submerged mycelium, water extract of the mycelium and total fraction of the water-soluble polysaccharides demonstrated the antitumor activity against murine lymphocytic leukemia P 388 in vivo.
29558056	10	41	from	capacity	1396:1403	arg1	F.velutipes					1446:1456	F.velutipes	1446:1456	F.velutipes	1446:1456	The antioxidant capacity (AOC) of the water-soluble fraction from F.velutipes was higher than that of the water-soluble polysaccharides from the sub- merged mycelium of Grifolafrondosa, but inferior to the AOC of the water-soluble polysaccharides from the submerged mycelium of Ganoderma luciduma.
29558056	7	42	theme	antitumor	1030:1038	arg1	activity					1040:1047	The antitumor activity	1026:1047	The antitumor activity of the substances	1026:1065	The antitumor activity of the substances was mainly due to the polysaccharides, since their purification increased the tumor growth inhibition.
29558056	7	42	theme	antitumor	1030:1038	arg1	due					1078:1080	due	1078:1080	due	1078:1080	The antitumor activity of the substances was mainly due to the polysaccharides, since their purification increased the tumor growth inhibition.
29558056	3	43	theme	Fv-1	531:534	arg1	mycelium					500:507	The submerged mycelium	486:507	The submerged mycelium of F.velutipes strain Fv-1	486:534	The submerged mycelium of F.velutipes strain Fv-1 contained 14.8% of a water-soluble polysaccharides, 31.6% of proteins, 2.5% of total lipids, vitamins B (B1, B5, B6).
29558056	1	44	theme	antioxidant	170:180	arg1	properties					182:191	antitumor and antioxidant properties	156:191	antitumor and antioxidant properties of water-soluble polysaccharides from submerged myceliumn of Flammulina velutipes grown under optimized conditions	156:306	The aim of the study was to evaluate antitumor and antioxidant properties of water-soluble polysaccharides from submerged myceliumn of Flammulina velutipes grown under optimized conditions.
29558056	6	45	theme	Dry	815:817	arg1	powder					819:824	Dry powder	815:824	Dry powder of the submerged mycelium	815:850	Dry powder of the submerged mycelium, water extract of the mycelium and total fraction of the water-soluble polysaccharides demonstrated the antitumor activity against murine lymphocytic leukemia P 388 in vivo.
29558056	8	46	theme	maximum	1174:1180	arg1	inhibition					1195:1204	The maximum tumor growth inhibition	1170:1204	The maximum tumor growth inhibition by the water-soluble polysaccharides	1170:1241	The maximum tumor growth inhibition by the water-soluble polysaccharides amounted to 94%.
29558056	3	47	theme	water-soluble	557:569	arg1	polysaccharides					571:585	a water-soluble polysaccharides	555:585	a water-soluble polysaccharides	555:585	The submerged mycelium of F.velutipes strain Fv-1 contained 14.8% of a water-soluble polysaccharides, 31.6% of proteins, 2.5% of total lipids, vitamins B (B1, B5, B6).
29558056	9	48	from	fraction	1270:1277	arg1	Fv-1					1340:1343	F.velutipes strain Fv-1	1321:1343	F.velutipes strain Fv-1	1321:1343	The total fraction of the water-soluble polysaccharides from F.velutipes strain Fv-1 demonstrated antioxidant activity.
29558056	8	49	theme	growth	1188:1193	arg1	inhibition					1195:1204	The maximum tumor growth inhibition	1170:1204	The maximum tumor growth inhibition by the water-soluble polysaccharides	1170:1241	The maximum tumor growth inhibition by the water-soluble polysaccharides amounted to 94%.
29558056	2	50	theme	medium	342:347	arg1	composition					349:359	the nutrient medium composition	329:359	the nutrient medium composition	329:359	The optimization of the nutrient medium composition allowed to increase the biomass yield by more than 2 times (up to 35 g/l) and to reduce the time of the cultivation process.
29558056	1	51	theme	polysaccharides	210:224	arg1	properties					182:191	antitumor and antioxidant properties	156:191	antitumor and antioxidant properties of water-soluble polysaccharides from submerged myceliumn of Flammulina velutipes grown under optimized conditions	156:306	The aim of the study was to evaluate antitumor and antioxidant properties of water-soluble polysaccharides from submerged myceliumn of Flammulina velutipes grown under optimized conditions.
29558056	2	52	theme	composition	349:359	arg1	optimization					313:324	The optimization	309:324	The optimization of the nutrient medium composition	309:359	The optimization of the nutrient medium composition allowed to increase the biomass yield by more than 2 times (up to 35 g/l) and to reduce the time of the cultivation process.
29558056	5	53	contain	contained	752:760	arg1	proteins					743:750	The proteins	739:750	The proteins	739:750	The proteins contained all the essential amino acids except for tryptophan.
29558056	5	53	contain	contained	752:760	arg2	acids					786:790	the essential amino acids	766:790	the essential amino acids	766:790	The proteins contained all the essential amino acids except for tryptophan.
29558056	10	54	theme	water-soluble	1597:1609	arg1	polysaccharides					1611:1625	the water-soluble polysaccharides	1593:1625	the water-soluble polysaccharides from the submerged mycelium of Ganoderma luciduma	1593:1675	The antioxidant capacity (AOC) of the water-soluble fraction from F.velutipes was higher than that of the water-soluble polysaccharides from the sub- merged mycelium of Grifolafrondosa, but inferior to the AOC of the water-soluble polysaccharides from the submerged mycelium of Ganoderma luciduma.
29558056	5	55	theme	essential	770:778	arg1	acids					786:790	the essential amino acids	766:790	the essential amino acids	766:790	The proteins contained all the essential amino acids except for tryptophan.
29558056	0	56	from	Mycelium	85:92	arg1	Antitumor					0:8	Antitumor	0:8	Antitumor	0:8	Antitumor and Antioxidant Properties of Water-Soluble Polysaccharides from Submerged Mycelium of Flammulina velutipes.
29558056	0	56	from	Mycelium	85:92	arg1	Properties					26:35	Antioxidant Properties	14:35	Antioxidant Properties	14:35	Antitumor and Antioxidant Properties of Water-Soluble Polysaccharides from Submerged Mycelium of Flammulina velutipes.
29558056	0	56	from	Mycelium	85:92	arg1	Polysaccharides					54:68	Water-Soluble Polysaccharides	40:68	Water-Soluble Polysaccharides from Submerged Mycelium of Flammulina velutipes	40:116	Antitumor and Antioxidant Properties of Water-Soluble Polysaccharides from Submerged Mycelium of Flammulina velutipes.
29558056	8	57	theme	water-soluble	1213:1225	arg1	polysaccharides					1227:1241	the water-soluble polysaccharides	1209:1241	the water-soluble polysaccharides	1209:1241	The maximum tumor growth inhibition by the water-soluble polysaccharides amounted to 94%.
29558056	1	58	from	myceliumn	241:249	arg1	properties					182:191	antitumor and antioxidant properties	156:191	antitumor and antioxidant properties of water-soluble polysaccharides from submerged myceliumn of Flammulina velutipes grown under optimized conditions	156:306	The aim of the study was to evaluate antitumor and antioxidant properties of water-soluble polysaccharides from submerged myceliumn of Flammulina velutipes grown under optimized conditions.
29558056	1	58	from	myceliumn	241:249	arg1	polysaccharides					210:224	water-soluble polysaccharides	196:224	water-soluble polysaccharides from submerged myceliumn of Flammulina velutipes grown under optimized conditions	196:306	The aim of the study was to evaluate antitumor and antioxidant properties of water-soluble polysaccharides from submerged myceliumn of Flammulina velutipes grown under optimized conditions.
29558056	10	59	from	F.velutipes	1446:1456	arg1	fraction					1432:1439	the water-soluble fraction	1414:1439	the water-soluble fraction from F.velutipes	1414:1456	The antioxidant capacity (AOC) of the water-soluble fraction from F.velutipes was higher than that of the water-soluble polysaccharides from the sub- merged mycelium of Grifolafrondosa, but inferior to the AOC of the water-soluble polysaccharides from the submerged mycelium of Ganoderma luciduma.
29558056	10	59	from	F.velutipes	1446:1456	arg1	AOC					1406:1408	AOC	1406:1408	AOC	1406:1408	The antioxidant capacity (AOC) of the water-soluble fraction from F.velutipes was higher than that of the water-soluble polysaccharides from the sub- merged mycelium of Grifolafrondosa, but inferior to the AOC of the water-soluble polysaccharides from the submerged mycelium of Ganoderma luciduma.
29558056	10	59	from	F.velutipes	1446:1456	arg1	capacity					1396:1403	The antioxidant capacity	1380:1403	The antioxidant capacity (AOC) of the water-soluble fraction from F.velutipes	1380:1456	The antioxidant capacity (AOC) of the water-soluble fraction from F.velutipes was higher than that of the water-soluble polysaccharides from the sub- merged mycelium of Grifolafrondosa, but inferior to the AOC of the water-soluble polysaccharides from the submerged mycelium of Ganoderma luciduma.
29558056	10	59	from	F.velutipes	1446:1456	arg1	higher					1462:1467	higher	1462:1467	higher	1462:1467	The antioxidant capacity (AOC) of the water-soluble fraction from F.velutipes was higher than that of the water-soluble polysaccharides from the sub- merged mycelium of Grifolafrondosa, but inferior to the AOC of the water-soluble polysaccharides from the submerged mycelium of Ganoderma luciduma.
29558056	1	60	theme	study	134:138	arg1	aim					123:125	The aim	119:125	The aim of the study	119:138	The aim of the study was to evaluate antitumor and antioxidant properties of water-soluble polysaccharides from submerged myceliumn of Flammulina velutipes grown under optimized conditions.
29558056	3	61	theme	proteins	597:604	arg1	%					592:592	31.6%	588:592	31.6% of proteins	588:604	The submerged mycelium of F.velutipes strain Fv-1 contained 14.8% of a water-soluble polysaccharides, 31.6% of proteins, 2.5% of total lipids, vitamins B (B1, B5, B6).
29558056	3	61	theme	proteins	597:604	arg1	%					550:550	14.8%	546:550	14.8% of a water-soluble polysaccharides	546:585	The submerged mycelium of F.velutipes strain Fv-1 contained 14.8% of a water-soluble polysaccharides, 31.6% of proteins, 2.5% of total lipids, vitamins B (B1, B5, B6).
29558056	3	61	theme	proteins	597:604	arg1	proteins					597:604	proteins	597:604	proteins	597:604	The submerged mycelium of F.velutipes strain Fv-1 contained 14.8% of a water-soluble polysaccharides, 31.6% of proteins, 2.5% of total lipids, vitamins B (B1, B5, B6).
29558056	3	61	theme	proteins	597:604	arg1	polysaccharides					571:585	a water-soluble polysaccharides	555:585	a water-soluble polysaccharides	555:585	The submerged mycelium of F.velutipes strain Fv-1 contained 14.8% of a water-soluble polysaccharides, 31.6% of proteins, 2.5% of total lipids, vitamins B (B1, B5, B6).
29558056	0	62	from	Properties	26:35	arg1	Mycelium					85:92	Submerged Mycelium	75:92	Submerged Mycelium of Flammulina velutipes	75:116	Antitumor and Antioxidant Properties of Water-Soluble Polysaccharides from Submerged Mycelium of Flammulina velutipes.
29558056	7	63	theme	tumor	1145:1149	arg1	inhibition					1158:1167	the tumor growth inhibition	1141:1167	the tumor growth inhibition	1141:1167	The antitumor activity of the substances was mainly due to the polysaccharides, since their purification increased the tumor growth inhibition.
29558056	0	64	theme	Flammulina	97:106	arg1	velutipes					108:116	Flammulina velutipes	97:116	Flammulina velutipes	97:116	Antitumor and Antioxidant Properties of Water-Soluble Polysaccharides from Submerged Mycelium of Flammulina velutipes.
29558056	1	65	from	properties	182:191	arg1	myceliumn					241:249	submerged myceliumn	231:249	submerged myceliumn of Flammulina velutipes grown under optimized conditions	231:306	The aim of the study was to evaluate antitumor and antioxidant properties of water-soluble polysaccharides from submerged myceliumn of Flammulina velutipes grown under optimized conditions.
29558056	1	66	theme	antitumor	156:164	arg1	properties					182:191	antitumor and antioxidant properties	156:191	antitumor and antioxidant properties of water-soluble polysaccharides from submerged myceliumn of Flammulina velutipes grown under optimized conditions	156:306	The aim of the study was to evaluate antitumor and antioxidant properties of water-soluble polysaccharides from submerged myceliumn of Flammulina velutipes grown under optimized conditions.
29558056	6	67	theme	polysaccharides	923:937	arg1	powder					819:824	Dry powder	815:824	Dry powder of the submerged mycelium	815:850	Dry powder of the submerged mycelium, water extract of the mycelium and total fraction of the water-soluble polysaccharides demonstrated the antitumor activity against murine lymphocytic leukemia P 388 in vivo.
29558056	6	67	theme	polysaccharides	923:937	arg1	extract					859:865	water extract	853:865	water extract of the mycelium	853:881	Dry powder of the submerged mycelium, water extract of the mycelium and total fraction of the water-soluble polysaccharides demonstrated the antitumor activity against murine lymphocytic leukemia P 388 in vivo.
29558056	6	67	theme	polysaccharides	923:937	arg1	fraction					893:900	total fraction	887:900	total fraction of the water-soluble polysaccharides	887:937	Dry powder of the submerged mycelium, water extract of the mycelium and total fraction of the water-soluble polysaccharides demonstrated the antitumor activity against murine lymphocytic leukemia P 388 in vivo.
29558056	2	68	theme	process	477:483	arg1	time					453:456	the time	449:456	the time of the cultivation process	449:483	The optimization of the nutrient medium composition allowed to increase the biomass yield by more than 2 times (up to 35 g/l) and to reduce the time of the cultivation process.
29558056	1	69	theme	Flammulina	254:263	arg1	velutipes					265:273	Flammulina velutipes	254:273	Flammulina velutipes grown under optimized conditions	254:306	The aim of the study was to evaluate antitumor and antioxidant properties of water-soluble polysaccharides from submerged myceliumn of Flammulina velutipes grown under optimized conditions.
29558056	10	70	theme	water-soluble	1418:1430	arg1	fraction					1432:1439	the water-soluble fraction	1414:1439	the water-soluble fraction from F.velutipes	1414:1456	The antioxidant capacity (AOC) of the water-soluble fraction from F.velutipes was higher than that of the water-soluble polysaccharides from the sub- merged mycelium of Grifolafrondosa, but inferior to the AOC of the water-soluble polysaccharides from the submerged mycelium of Ganoderma luciduma.
29558056	10	71	theme	polysaccharides	1611:1625	arg1	AOC					1586:1588	the AOC	1582:1588	the AOC of the water-soluble polysaccharides from the submerged mycelium of Ganoderma luciduma	1582:1675	The antioxidant capacity (AOC) of the water-soluble fraction from F.velutipes was higher than that of the water-soluble polysaccharides from the sub- merged mycelium of Grifolafrondosa, but inferior to the AOC of the water-soluble polysaccharides from the submerged mycelium of Ganoderma luciduma.
29558056	6	72	theme	leukemia	1002:1009	arg1	388					1013:1015	murine lymphocytic leukemia P 388	983:1015	murine lymphocytic leukemia P 388	983:1015	Dry powder of the submerged mycelium, water extract of the mycelium and total fraction of the water-soluble polysaccharides demonstrated the antitumor activity against murine lymphocytic leukemia P 388 in vivo.
29558056	3	73	theme	lipids	621:626	arg1	lipids					621:626	total lipids	615:626	total lipids	615:626	The submerged mycelium of F.velutipes strain Fv-1 contained 14.8% of a water-soluble polysaccharides, 31.6% of proteins, 2.5% of total lipids, vitamins B (B1, B5, B6).
29558056	3	73	theme	lipids	621:626	arg1	%					550:550	14.8%	546:550	14.8% of a water-soluble polysaccharides	546:585	The submerged mycelium of F.velutipes strain Fv-1 contained 14.8% of a water-soluble polysaccharides, 31.6% of proteins, 2.5% of total lipids, vitamins B (B1, B5, B6).
29558056	3	73	theme	lipids	621:626	arg1	polysaccharides					571:585	a water-soluble polysaccharides	555:585	a water-soluble polysaccharides	555:585	The submerged mycelium of F.velutipes strain Fv-1 contained 14.8% of a water-soluble polysaccharides, 31.6% of proteins, 2.5% of total lipids, vitamins B (B1, B5, B6).
29558056	3	73	theme	lipids	621:626	arg1	%					610:610	2.5%	607:610	2.5% of total lipids	607:626	The submerged mycelium of F.velutipes strain Fv-1 contained 14.8% of a water-soluble polysaccharides, 31.6% of proteins, 2.5% of total lipids, vitamins B (B1, B5, B6).
29558056	10	74	theme	Ganoderma	1658:1666	arg1	luciduma					1668:1675	Ganoderma luciduma	1658:1675	Ganoderma luciduma	1658:1675	The antioxidant capacity (AOC) of the water-soluble fraction from F.velutipes was higher than that of the water-soluble polysaccharides from the sub- merged mycelium of Grifolafrondosa, but inferior to the AOC of the water-soluble polysaccharides from the submerged mycelium of Ganoderma luciduma.
29558056	0	75	theme	Water-Soluble	40:52	arg1	Polysaccharides					54:68	Water-Soluble Polysaccharides	40:68	Water-Soluble Polysaccharides from Submerged Mycelium of Flammulina velutipes	40:116	Antitumor and Antioxidant Properties of Water-Soluble Polysaccharides from Submerged Mycelium of Flammulina velutipes.
29558056	9	76	theme	polysaccharides	1300:1314	arg1	fraction					1270:1277	The total fraction	1260:1277	The total fraction of the water-soluble polysaccharides from F.velutipes strain Fv-1	1260:1343	The total fraction of the water-soluble polysaccharides from F.velutipes strain Fv-1 demonstrated antioxidant activity.
29558056	1	77	theme	submerged	231:239	arg1	myceliumn					241:249	submerged myceliumn	231:249	submerged myceliumn of Flammulina velutipes grown under optimized conditions	231:306	The aim of the study was to evaluate antitumor and antioxidant properties of water-soluble polysaccharides from submerged myceliumn of Flammulina velutipes grown under optimized conditions.
29558056	6	78	theme	murine	983:988	arg1	leukemia					1002:1009	murine lymphocytic leukemia	983:1009	murine lymphocytic leukemia P 388	983:1015	Dry powder of the submerged mycelium, water extract of the mycelium and total fraction of the water-soluble polysaccharides demonstrated the antitumor activity against murine lymphocytic leukemia P 388 in vivo.
29558056	3	79	contain	contained	536:544	arg2	%					610:610	2.5%	607:610	2.5% of total lipids	607:626	The submerged mycelium of F.velutipes strain Fv-1 contained 14.8% of a water-soluble polysaccharides, 31.6% of proteins, 2.5% of total lipids, vitamins B (B1, B5, B6).
29558056	3	79	contain	contained	536:544	arg2	B					638:638	vitamins B	629:638	vitamins B (B1, B5, B6)	629:651	The submerged mycelium of F.velutipes strain Fv-1 contained 14.8% of a water-soluble polysaccharides, 31.6% of proteins, 2.5% of total lipids, vitamins B (B1, B5, B6).
29558056	3	79	contain	contained	536:544	arg2	%					550:550	14.8%	546:550	14.8% of a water-soluble polysaccharides	546:585	The submerged mycelium of F.velutipes strain Fv-1 contained 14.8% of a water-soluble polysaccharides, 31.6% of proteins, 2.5% of total lipids, vitamins B (B1, B5, B6).
29558056	3	79	contain	contained	536:544	arg2	%					592:592	31.6%	588:592	31.6% of proteins	588:604	The submerged mycelium of F.velutipes strain Fv-1 contained 14.8% of a water-soluble polysaccharides, 31.6% of proteins, 2.5% of total lipids, vitamins B (B1, B5, B6).
29558056	3	79	contain	contained	536:544	arg2	polysaccharides					571:585	a water-soluble polysaccharides	555:585	a water-soluble polysaccharides	555:585	The submerged mycelium of F.velutipes strain Fv-1 contained 14.8% of a water-soluble polysaccharides, 31.6% of proteins, 2.5% of total lipids, vitamins B (B1, B5, B6).
29558056	3	79	contain	contained	536:544	arg2	proteins					597:604	proteins	597:604	proteins	597:604	The submerged mycelium of F.velutipes strain Fv-1 contained 14.8% of a water-soluble polysaccharides, 31.6% of proteins, 2.5% of total lipids, vitamins B (B1, B5, B6).
29558056	3	79	contain	contained	536:544	arg1	mycelium					500:507	The submerged mycelium	486:507	The submerged mycelium of F.velutipes strain Fv-1	486:534	The submerged mycelium of F.velutipes strain Fv-1 contained 14.8% of a water-soluble polysaccharides, 31.6% of proteins, 2.5% of total lipids, vitamins B (B1, B5, B6).
29558056	3	79	contain	contained	536:544	arg2	lipids					621:626	total lipids	615:626	total lipids	615:626	The submerged mycelium of F.velutipes strain Fv-1 contained 14.8% of a water-soluble polysaccharides, 31.6% of proteins, 2.5% of total lipids, vitamins B (B1, B5, B6).
29558056	9	80	theme	F.velutipes	1321:1331	arg1	Fv-1					1340:1343	F.velutipes strain Fv-1	1321:1343	F.velutipes strain Fv-1	1321:1343	The total fraction of the water-soluble polysaccharides from F.velutipes strain Fv-1 demonstrated antioxidant activity.
29558056	10	81	theme	water-soluble	1486:1498	arg1	polysaccharides					1500:1514	the water-soluble polysaccharides	1482:1514	the water-soluble polysaccharides from the sub- merged mycelium of Grifolafrondosa	1482:1563	The antioxidant capacity (AOC) of the water-soluble fraction from F.velutipes was higher than that of the water-soluble polysaccharides from the sub- merged mycelium of Grifolafrondosa, but inferior to the AOC of the water-soluble polysaccharides from the submerged mycelium of Ganoderma luciduma.
29558056	3	82	theme	submerged	490:498	arg1	mycelium					500:507	The submerged mycelium	486:507	The submerged mycelium of F.velutipes strain Fv-1	486:534	The submerged mycelium of F.velutipes strain Fv-1 contained 14.8% of a water-soluble polysaccharides, 31.6% of proteins, 2.5% of total lipids, vitamins B (B1, B5, B6).
29558056	10	83	from	mycelium	1537:1544	arg1	polysaccharides					1500:1514	the water-soluble polysaccharides	1482:1514	the water-soluble polysaccharides from the sub- merged mycelium of Grifolafrondosa	1482:1563	The antioxidant capacity (AOC) of the water-soluble fraction from F.velutipes was higher than that of the water-soluble polysaccharides from the sub- merged mycelium of Grifolafrondosa, but inferior to the AOC of the water-soluble polysaccharides from the submerged mycelium of Ganoderma luciduma.
29558056	3	84	theme	vitamins	629:636	arg1	B					638:638	vitamins B	629:638	vitamins B (B1, B5, B6)	629:651	The submerged mycelium of F.velutipes strain Fv-1 contained 14.8% of a water-soluble polysaccharides, 31.6% of proteins, 2.5% of total lipids, vitamins B (B1, B5, B6).
29558056	3	84	theme	vitamins	629:636	arg1	%					550:550	14.8%	546:550	14.8% of a water-soluble polysaccharides	546:585	The submerged mycelium of F.velutipes strain Fv-1 contained 14.8% of a water-soluble polysaccharides, 31.6% of proteins, 2.5% of total lipids, vitamins B (B1, B5, B6).
29558056	3	84	theme	vitamins	629:636	arg1	polysaccharides					571:585	a water-soluble polysaccharides	555:585	a water-soluble polysaccharides	555:585	The submerged mycelium of F.velutipes strain Fv-1 contained 14.8% of a water-soluble polysaccharides, 31.6% of proteins, 2.5% of total lipids, vitamins B (B1, B5, B6).
29558056	9	85	theme	antioxidant	1358:1368	arg1	activity					1370:1377	antioxidant activity	1358:1377	antioxidant activity	1358:1377	The total fraction of the water-soluble polysaccharides from F.velutipes strain Fv-1 demonstrated antioxidant activity.
29558056	10	86	from	mycelium	1646:1653	arg1	polysaccharides					1611:1625	the water-soluble polysaccharides	1593:1625	the water-soluble polysaccharides from the submerged mycelium of Ganoderma luciduma	1593:1675	The antioxidant capacity (AOC) of the water-soluble fraction from F.velutipes was higher than that of the water-soluble polysaccharides from the sub- merged mycelium of Grifolafrondosa, but inferior to the AOC of the water-soluble polysaccharides from the submerged mycelium of Ganoderma luciduma.
29558056	10	86	from	mycelium	1646:1653	arg1	AOC					1586:1588	the AOC	1582:1588	the AOC of the water-soluble polysaccharides from the submerged mycelium of Ganoderma luciduma	1582:1675	The antioxidant capacity (AOC) of the water-soluble fraction from F.velutipes was higher than that of the water-soluble polysaccharides from the sub- merged mycelium of Grifolafrondosa, but inferior to the AOC of the water-soluble polysaccharides from the submerged mycelium of Ganoderma luciduma.
29558056	10	87	theme	sub-	1525:1528	arg1	mycelium					1537:1544	the sub- merged mycelium	1521:1544	the sub- merged mycelium of Grifolafrondosa	1521:1563	The antioxidant capacity (AOC) of the water-soluble fraction from F.velutipes was higher than that of the water-soluble polysaccharides from the sub- merged mycelium of Grifolafrondosa, but inferior to the AOC of the water-soluble polysaccharides from the submerged mycelium of Ganoderma luciduma.
29558056	3	88	theme	strain	524:529	arg1	Fv-1					531:534	F.velutipes strain Fv-1	512:534	F.velutipes strain Fv-1	512:534	The submerged mycelium of F.velutipes strain Fv-1 contained 14.8% of a water-soluble polysaccharides, 31.6% of proteins, 2.5% of total lipids, vitamins B (B1, B5, B6).
25965463	4	0	theme	loading	461:467	arg1	effect					445:450	The effect	441:450	The effect of fiber loading on the tensile properties and thermal stability	441:515	The effect of fiber loading on the tensile properties and thermal stability was studied.
25965463	1	1	theme	hydroxide+12	156:167	arg1	urea					170:173	aq.7% sodium hydroxide+12% urea	143:173	aq.7% sodium hydroxide+12% urea	143:173	Cellulose was dissolved in pre cooled environment friendly solvent (aq.7% sodium hydroxide+12% urea) and regenerated with 5%H2SO4 as coagulation bath.
25965463	1	1	theme	hydroxide+12	156:167	arg1	solvent					134:140	pre cooled environment friendly solvent	102:140	pre cooled environment friendly solvent (aq.7% sodium hydroxide+12% urea)	102:174	Cellulose was dissolved in pre cooled environment friendly solvent (aq.7% sodium hydroxide+12% urea) and regenerated with 5%H2SO4 as coagulation bath.
25965463	4	2	theme	tensile	476:482	arg1	properties					484:493	the tensile properties	472:493	the tensile properties	472:493	The effect of fiber loading on the tensile properties and thermal stability was studied.
25965463	1	3	theme	%	168:168	arg1	urea					170:173	aq.7% sodium hydroxide+12% urea	143:173	aq.7% sodium hydroxide+12% urea	143:173	Cellulose was dissolved in pre cooled environment friendly solvent (aq.7% sodium hydroxide+12% urea) and regenerated with 5%H2SO4 as coagulation bath.
25965463	1	3	theme	%	168:168	arg1	solvent					134:140	pre cooled environment friendly solvent	102:140	pre cooled environment friendly solvent (aq.7% sodium hydroxide+12% urea)	102:174	Cellulose was dissolved in pre cooled environment friendly solvent (aq.7% sodium hydroxide+12% urea) and regenerated with 5%H2SO4 as coagulation bath.
25965463	2	4	theme	lampas	338:343	arg1	plant					345:349	the newly identified Thespesia lampas plant	307:349	the newly identified Thespesia lampas plant	307:349	Using cellulose as matrix and alkali treated short natural fibers extracted from the newly identified Thespesia lampas plant as fillers the green composite films were prepared.
25965463	2	5	theme	short	271:275	arg1	fibers					285:290	alkali treated short natural fibers	256:290	alkali treated short natural fibers	256:290	Using cellulose as matrix and alkali treated short natural fibers extracted from the newly identified Thespesia lampas plant as fillers the green composite films were prepared.
25965463	2	5	theme	short	271:275	arg1	cellulose					232:240	cellulose	232:240	cellulose	232:240	Using cellulose as matrix and alkali treated short natural fibers extracted from the newly identified Thespesia lampas plant as fillers the green composite films were prepared.
25965463	2	6	theme	natural	277:283	arg1	fibers					285:290	alkali treated short natural fibers	256:290	alkali treated short natural fibers	256:290	Using cellulose as matrix and alkali treated short natural fibers extracted from the newly identified Thespesia lampas plant as fillers the green composite films were prepared.
25965463	2	6	theme	natural	277:283	arg1	cellulose					232:240	cellulose	232:240	cellulose	232:240	Using cellulose as matrix and alkali treated short natural fibers extracted from the newly identified Thespesia lampas plant as fillers the green composite films were prepared.
25965463	8	7	theme	fiber	1098:1102	arg1	content					1104:1110	fiber content	1098:1110	fiber content	1098:1110	The thermal stability of the composite films was higher than the matrix and increased with fiber content.
25965463	2	8	theme	treated	263:269	arg1	fibers					285:290	alkali treated short natural fibers	256:290	alkali treated short natural fibers	256:290	Using cellulose as matrix and alkali treated short natural fibers extracted from the newly identified Thespesia lampas plant as fillers the green composite films were prepared.
25965463	2	8	theme	treated	263:269	arg1	cellulose					232:240	cellulose	232:240	cellulose	232:240	Using cellulose as matrix and alkali treated short natural fibers extracted from the newly identified Thespesia lampas plant as fillers the green composite films were prepared.
25965463	1	9	theme	friendly	125:132	arg1	urea					170:173	aq.7% sodium hydroxide+12% urea	143:173	aq.7% sodium hydroxide+12% urea	143:173	Cellulose was dissolved in pre cooled environment friendly solvent (aq.7% sodium hydroxide+12% urea) and regenerated with 5%H2SO4 as coagulation bath.
25965463	1	9	theme	friendly	125:132	arg1	solvent					134:140	pre cooled environment friendly solvent	102:140	pre cooled environment friendly solvent (aq.7% sodium hydroxide+12% urea)	102:174	Cellulose was dissolved in pre cooled environment friendly solvent (aq.7% sodium hydroxide+12% urea) and regenerated with 5%H2SO4 as coagulation bath.
25965463	7	10	from	orientation	962:972	arg1	composites					995:1004	the composites	991:1004	the composites	991:1004	In spite of better interfacial bonding, the tensile properties of the composites were found to be lower than those of the matrix and decreased with increasing fiber content and this behavior was attributed to the random orientation of the fibers in the composites.
25965463	2	11	theme	alkali	256:261	arg1	fibers					285:290	alkali treated short natural fibers	256:290	alkali treated short natural fibers	256:290	Using cellulose as matrix and alkali treated short natural fibers extracted from the newly identified Thespesia lampas plant as fillers the green composite films were prepared.
25965463	2	11	theme	alkali	256:261	arg1	cellulose					232:240	cellulose	232:240	cellulose	232:240	Using cellulose as matrix and alkali treated short natural fibers extracted from the newly identified Thespesia lampas plant as fillers the green composite films were prepared.
25965463	4	12	theme	fiber	455:459	arg1	loading					461:467	fiber loading	455:467	fiber loading	455:467	The effect of fiber loading on the tensile properties and thermal stability was studied.
25965463	7	13	theme	composites	812:821	arg1	properties					794:803	the tensile properties	782:803	the tensile properties of the composites	782:821	In spite of better interfacial bonding, the tensile properties of the composites were found to be lower than those of the matrix and decreased with increasing fiber content and this behavior was attributed to the random orientation of the fibers in the composites.
25965463	7	13	theme	composites	812:821	arg1	lower					840:844	lower	840:844	lower	840:844	In spite of better interfacial bonding, the tensile properties of the composites were found to be lower than those of the matrix and decreased with increasing fiber content and this behavior was attributed to the random orientation of the fibers in the composites.
25965463	2	14	theme	composite	372:380	arg1	films					382:386	the green composite films	362:386	the green composite films	362:386	Using cellulose as matrix and alkali treated short natural fibers extracted from the newly identified Thespesia lampas plant as fillers the green composite films were prepared.
25965463	4	15	from	effect	445:450	arg1	properties					484:493	the tensile properties	472:493	the tensile properties	472:493	The effect of fiber loading on the tensile properties and thermal stability was studied.
25965463	4	15	from	effect	445:450	arg1	stability					507:515	thermal stability	499:515	thermal stability	499:515	The effect of fiber loading on the tensile properties and thermal stability was studied.
25965463	7	16	theme	fiber	901:905	arg1	content					907:913	fiber content	901:913	fiber content	901:913	In spite of better interfacial bonding, the tensile properties of the composites were found to be lower than those of the matrix and decreased with increasing fiber content and this behavior was attributed to the random orientation of the fibers in the composites.
25965463	8	17	theme	thermal	1011:1017	arg1	higher					1056:1061	higher	1056:1061	higher	1056:1061	The thermal stability of the composite films was higher than the matrix and increased with fiber content.
25965463	8	17	theme	thermal	1011:1017	arg1	stability					1019:1027	The thermal stability	1007:1027	The thermal stability of the composite films	1007:1050	The thermal stability of the composite films was higher than the matrix and increased with fiber content.
25965463	5	18	theme	better	557:562	arg1	bonding					576:582	better interfacial bonding	557:582	better interfacial bonding between the fibers and cellulose	557:615	The fractographs indicated better interfacial bonding between the fibers and cellulose.
25965463	1	19	theme	pre	102:104	arg1	urea					170:173	aq.7% sodium hydroxide+12% urea	143:173	aq.7% sodium hydroxide+12% urea	143:173	Cellulose was dissolved in pre cooled environment friendly solvent (aq.7% sodium hydroxide+12% urea) and regenerated with 5%H2SO4 as coagulation bath.
25965463	1	19	theme	pre	102:104	arg1	solvent					134:140	pre cooled environment friendly solvent	102:140	pre cooled environment friendly solvent (aq.7% sodium hydroxide+12% urea)	102:174	Cellulose was dissolved in pre cooled environment friendly solvent (aq.7% sodium hydroxide+12% urea) and regenerated with 5%H2SO4 as coagulation bath.
25965463	0	20	theme	lampas	34:39	arg1	fibers					47:52	cellulose/Thespesia lampas short fibers	14:52	cellulose/Thespesia lampas short fibers	14:52	Properties of cellulose/Thespesia lampas short fibers bio-composite films.
25965463	5	21	theme	interfacial	564:574	arg1	bonding					576:582	better interfacial bonding	557:582	better interfacial bonding between the fibers and cellulose	557:615	The fractographs indicated better interfacial bonding between the fibers and cellulose.
25965463	1	22	theme	cooled	106:111	arg1	urea					170:173	aq.7% sodium hydroxide+12% urea	143:173	aq.7% sodium hydroxide+12% urea	143:173	Cellulose was dissolved in pre cooled environment friendly solvent (aq.7% sodium hydroxide+12% urea) and regenerated with 5%H2SO4 as coagulation bath.
25965463	1	22	theme	cooled	106:111	arg1	solvent					134:140	pre cooled environment friendly solvent	102:140	pre cooled environment friendly solvent (aq.7% sodium hydroxide+12% urea)	102:174	Cellulose was dissolved in pre cooled environment friendly solvent (aq.7% sodium hydroxide+12% urea) and regenerated with 5%H2SO4 as coagulation bath.
25965463	0	23	theme	cellulose/Thespesia	14:32	arg1	fibers					47:52	cellulose/Thespesia lampas short fibers	14:52	cellulose/Thespesia lampas short fibers	14:52	Properties of cellulose/Thespesia lampas short fibers bio-composite films.
25965463	7	24	theme	interfacial	761:771	arg1	bonding					773:779	better interfacial bonding	754:779	better interfacial bonding	754:779	In spite of better interfacial bonding, the tensile properties of the composites were found to be lower than those of the matrix and decreased with increasing fiber content and this behavior was attributed to the random orientation of the fibers in the composites.
25965463	7	25	theme	random	955:960	arg1	orientation					962:972	the random orientation	951:972	the random orientation of the fibers in the composites	951:1004	In spite of better interfacial bonding, the tensile properties of the composites were found to be lower than those of the matrix and decreased with increasing fiber content and this behavior was attributed to the random orientation of the fibers in the composites.
25965463	1	26	theme	environment	113:123	arg1	urea					170:173	aq.7% sodium hydroxide+12% urea	143:173	aq.7% sodium hydroxide+12% urea	143:173	Cellulose was dissolved in pre cooled environment friendly solvent (aq.7% sodium hydroxide+12% urea) and regenerated with 5%H2SO4 as coagulation bath.
25965463	1	26	theme	environment	113:123	arg1	solvent					134:140	pre cooled environment friendly solvent	102:140	pre cooled environment friendly solvent (aq.7% sodium hydroxide+12% urea)	102:174	Cellulose was dissolved in pre cooled environment friendly solvent (aq.7% sodium hydroxide+12% urea) and regenerated with 5%H2SO4 as coagulation bath.
25965463	7	27	theme	tensile	786:792	arg1	properties					794:803	the tensile properties	782:803	the tensile properties of the composites	782:821	In spite of better interfacial bonding, the tensile properties of the composites were found to be lower than those of the matrix and decreased with increasing fiber content and this behavior was attributed to the random orientation of the fibers in the composites.
25965463	7	27	theme	tensile	786:792	arg1	lower					840:844	lower	840:844	lower	840:844	In spite of better interfacial bonding, the tensile properties of the composites were found to be lower than those of the matrix and decreased with increasing fiber content and this behavior was attributed to the random orientation of the fibers in the composites.
25965463	1	28	theme	5	197:197	arg1	%					198:198	%	198:198	%	198:198	Cellulose was dissolved in pre cooled environment friendly solvent (aq.7% sodium hydroxide+12% urea) and regenerated with 5%H2SO4 as coagulation bath.
25965463	0	29	theme	fibers	47:52	arg1	Properties					0:9	Properties	0:9	Properties of cellulose/Thespesia lampas short fibers	0:52	Properties of cellulose/Thespesia lampas short fibers bio-composite films.
25965463	8	30	theme	films	1046:1050	arg1	higher					1056:1061	higher	1056:1061	higher	1056:1061	The thermal stability of the composite films was higher than the matrix and increased with fiber content.
25965463	8	30	theme	films	1046:1050	arg1	stability					1019:1027	The thermal stability	1007:1027	The thermal stability of the composite films	1007:1050	The thermal stability of the composite films was higher than the matrix and increased with fiber content.
25965463	2	31	dep	prepared	393:400	arg1	Using					226:230	Using	226:230	Using cellulose as matrix and alkali treated short natural fibers extracted from the newly identified Thespesia lampas plant as fillers	226:360	Using cellulose as matrix and alkali treated short natural fibers extracted from the newly identified Thespesia lampas plant as fillers the green composite films were prepared.
25965463	6	32	theme	films	653:657	arg1	lower					675:679	lower	675:679	lower	675:679	The crystallinity of the composite films was found to be lower than the matrix and decreased with increasing fiber content.
25965463	6	32	theme	films	653:657	arg1	crystallinity					622:634	The crystallinity	618:634	The crystallinity of the composite films	618:657	The crystallinity of the composite films was found to be lower than the matrix and decreased with increasing fiber content.
25965463	1	33	theme	%	198:198	arg1	H2SO4					199:203	5%H2SO4	197:203	5%H2SO4 as coagulation bath	197:223	Cellulose was dissolved in pre cooled environment friendly solvent (aq.7% sodium hydroxide+12% urea) and regenerated with 5%H2SO4 as coagulation bath.
25965463	0	34	theme	short	41:45	arg1	fibers					47:52	cellulose/Thespesia lampas short fibers	14:52	cellulose/Thespesia lampas short fibers	14:52	Properties of cellulose/Thespesia lampas short fibers bio-composite films.
25965463	8	35	theme	composite	1036:1044	arg1	films					1046:1050	the composite films	1032:1050	the composite films	1032:1050	The thermal stability of the composite films was higher than the matrix and increased with fiber content.
25965463	7	36	theme	better	754:759	arg1	bonding					773:779	better interfacial bonding	754:779	better interfacial bonding	754:779	In spite of better interfacial bonding, the tensile properties of the composites were found to be lower than those of the matrix and decreased with increasing fiber content and this behavior was attributed to the random orientation of the fibers in the composites.
25965463	2	37	theme	Thespesia	328:336	arg1	plant					345:349	the newly identified Thespesia lampas plant	307:349	the newly identified Thespesia lampas plant	307:349	Using cellulose as matrix and alkali treated short natural fibers extracted from the newly identified Thespesia lampas plant as fillers the green composite films were prepared.
25965463	6	38	theme	fiber	727:731	arg1	content					733:739	fiber content	727:739	fiber content	727:739	The crystallinity of the composite films was found to be lower than the matrix and decreased with increasing fiber content.
25965463	2	39	theme	identified	317:326	arg1	plant					345:349	the newly identified Thespesia lampas plant	307:349	the newly identified Thespesia lampas plant	307:349	Using cellulose as matrix and alkali treated short natural fibers extracted from the newly identified Thespesia lampas plant as fillers the green composite films were prepared.
25965463	6	40	theme	composite	643:651	arg1	films					653:657	the composite films	639:657	the composite films	639:657	The crystallinity of the composite films was found to be lower than the matrix and decreased with increasing fiber content.
25965463	4	41	theme	thermal	499:505	arg1	stability					507:515	thermal stability	499:515	thermal stability	499:515	The effect of fiber loading on the tensile properties and thermal stability was studied.
25965463	7	42	theme	fibers	981:986	arg1	orientation					962:972	the random orientation	951:972	the random orientation of the fibers in the composites	951:1004	In spite of better interfacial bonding, the tensile properties of the composites were found to be lower than those of the matrix and decreased with increasing fiber content and this behavior was attributed to the random orientation of the fibers in the composites.
25965463	7	43	from	fibers	981:986	arg1	composites					995:1004	the composites	991:1004	the composites	991:1004	In spite of better interfacial bonding, the tensile properties of the composites were found to be lower than those of the matrix and decreased with increasing fiber content and this behavior was attributed to the random orientation of the fibers in the composites.
25965463	1	44	theme	coagulation	208:218	arg1	bath					220:223	coagulation bath	208:223	coagulation bath	208:223	Cellulose was dissolved in pre cooled environment friendly solvent (aq.7% sodium hydroxide+12% urea) and regenerated with 5%H2SO4 as coagulation bath.
25965463	1	45	theme	%	147:147	arg1	urea					170:173	aq.7% sodium hydroxide+12% urea	143:173	aq.7% sodium hydroxide+12% urea	143:173	Cellulose was dissolved in pre cooled environment friendly solvent (aq.7% sodium hydroxide+12% urea) and regenerated with 5%H2SO4 as coagulation bath.
25965463	1	45	theme	%	147:147	arg1	solvent					134:140	pre cooled environment friendly solvent	102:140	pre cooled environment friendly solvent (aq.7% sodium hydroxide+12% urea)	102:174	Cellulose was dissolved in pre cooled environment friendly solvent (aq.7% sodium hydroxide+12% urea) and regenerated with 5%H2SO4 as coagulation bath.
25965463	1	46	theme	sodium	149:154	arg1	urea					170:173	aq.7% sodium hydroxide+12% urea	143:173	aq.7% sodium hydroxide+12% urea	143:173	Cellulose was dissolved in pre cooled environment friendly solvent (aq.7% sodium hydroxide+12% urea) and regenerated with 5%H2SO4 as coagulation bath.
25965463	1	46	theme	sodium	149:154	arg1	solvent					134:140	pre cooled environment friendly solvent	102:140	pre cooled environment friendly solvent (aq.7% sodium hydroxide+12% urea)	102:174	Cellulose was dissolved in pre cooled environment friendly solvent (aq.7% sodium hydroxide+12% urea) and regenerated with 5%H2SO4 as coagulation bath.
25965463	7	47	from	composites	995:1004	arg1	orientation					962:972	the random orientation	951:972	the random orientation of the fibers in the composites	951:1004	In spite of better interfacial bonding, the tensile properties of the composites were found to be lower than those of the matrix and decreased with increasing fiber content and this behavior was attributed to the random orientation of the fibers in the composites.
23996303	0	0	theme	capsule	93:99	arg1	integrity					58:66	the integrity	54:66	the integrity of Klebsiella pneumoniae capsule	54:99	Aeromonas punctata derived depolymerase that disrupts the integrity of Klebsiella pneumoniae capsule: optimization of depolymerase production.
23996303	6	1	theme	166.65 µmoles ml	1041:1056	arg1	-1					1066:1067	-1	1066:1067	-1	1066:1067	Optimization via central composite design (CCD) resulted in 16-fold enhancement in depolymerase yield (166.65 µmoles ml(-1) min(-1) ) over unoptimized medium.
23996303	6	1	theme	166.65 µmoles ml	1041:1056	arg1	min					1062:1064	166.65 µmoles ml(-1) min	1041:1064	166.65 µmoles ml(-1) min(-1)	1041:1068	Optimization via central composite design (CCD) resulted in 16-fold enhancement in depolymerase yield (166.65 µmoles ml(-1) min(-1) ) over unoptimized medium.
23996303	2	2	dep	resistance	328:337	arg1	compared					342:349	compared	342:349	compared to the planktonic cells	342:373	Extracellular polymeric substances encompassing biofilms offer 1000-fold greater resistance as compared to the planktonic cells.
23996303	6	3	dep	resulted	986:993	arg1	-1					1066:1067	-1	1066:1067	-1	1066:1067	Optimization via central composite design (CCD) resulted in 16-fold enhancement in depolymerase yield (166.65 µmoles ml(-1) min(-1) ) over unoptimized medium.
23996303	6	3	dep	resulted	986:993	arg1	min					1062:1064	166.65 µmoles ml(-1) min	1041:1064	166.65 µmoles ml(-1) min(-1)	1041:1068	Optimization via central composite design (CCD) resulted in 16-fold enhancement in depolymerase yield (166.65 µmoles ml(-1) min(-1) ) over unoptimized medium.
23996303	5	4	theme	Klebsiella	838:847	arg1	pneumoniae					849:858	Klebsiella pneumoniae B5055	838:864	Klebsiella pneumoniae B5055	838:864	Thus, statistical optimization was done to enhance depolymerase production by Aeromonas punctata, directed against the exopolysaccharide matrix of Klebsiella pneumoniae B5055, capable of substituting the available phage borne depolymerase enzyme.
23996303	5	5	theme	statistical	697:707	arg1	optimization					709:720	statistical optimization	697:720	statistical optimization	697:720	Thus, statistical optimization was done to enhance depolymerase production by Aeromonas punctata, directed against the exopolysaccharide matrix of Klebsiella pneumoniae B5055, capable of substituting the available phage borne depolymerase enzyme.
23996303	9	6	from	improvements	1542:1553	arg1	enzyme					1562:1567	the enzyme	1558:1567	the enzyme	1558:1567	Further improvements in the enzyme can lead to its successful development as prophylactic and/or a therapeutic agent.
23996303	8	7	with	homology	1461:1468	arg1	protein					1477:1483	a protein	1475:1483	a protein of unknown function from Aeromonas cavaie Ae398	1475:1531	Tryptic peptide analysis of the purified 29 kDa band by Matrix assisted laser desorption ionization-time of flight (MALDI-TOF) showed a high homology with a protein of unknown function from Aeromonas cavaie Ae398.
23996303	3	8	theme	anti-biofouling	393:407	arg1	enzymes					382:388	enzymes	382:388	enzymes	382:388	Using enzymes as anti-biofouling agents, will improve penetration of antimicrobials and increase susceptibility of biofilms to components of immune system.
23996303	3	8	theme	anti-biofouling	393:407	arg1	agents					409:414	anti-biofouling agents	393:414	anti-biofouling agents	393:414	Using enzymes as anti-biofouling agents, will improve penetration of antimicrobials and increase susceptibility of biofilms to components of immune system.
23996303	8	9	theme	cavaie	1520:1525	arg1	Ae398					1527:1531	Aeromonas cavaie Ae398	1510:1531	Aeromonas cavaie Ae398	1510:1531	Tryptic peptide analysis of the purified 29 kDa band by Matrix assisted laser desorption ionization-time of flight (MALDI-TOF) showed a high homology with a protein of unknown function from Aeromonas cavaie Ae398.
23996303	3	10	dep	improve	422:428	arg1	Using					376:380	Using	376:380	Using enzymes as anti-biofouling agents	376:414	Using enzymes as anti-biofouling agents, will improve penetration of antimicrobials and increase susceptibility of biofilms to components of immune system.
23996303	8	11	theme	29 kDa	1361:1366	arg1	band					1368:1371	the purified 29 kDa band	1348:1371	the purified 29 kDa band by Matrix assisted laser desorption ionization-time of flight (MALDI-TOF)	1348:1445	Tryptic peptide analysis of the purified 29 kDa band by Matrix assisted laser desorption ionization-time of flight (MALDI-TOF) showed a high homology with a protein of unknown function from Aeromonas cavaie Ae398.
23996303	9	12	theme	successful	1585:1594	arg1	development					1596:1606	its successful development	1581:1606	its successful development	1581:1606	Further improvements in the enzyme can lead to its successful development as prophylactic and/or a therapeutic agent.
23996303	8	13	theme	purified	1352:1359	arg1	band					1368:1371	the purified 29 kDa band	1348:1371	the purified 29 kDa band by Matrix assisted laser desorption ionization-time of flight (MALDI-TOF)	1348:1445	Tryptic peptide analysis of the purified 29 kDa band by Matrix assisted laser desorption ionization-time of flight (MALDI-TOF) showed a high homology with a protein of unknown function from Aeromonas cavaie Ae398.
23996303	2	14	theme	greater	320:326	arg1	resistance					328:337	1000-fold greater resistance	310:337	1000-fold greater resistance as compared to the planktonic cells	310:373	Extracellular polymeric substances encompassing biofilms offer 1000-fold greater resistance as compared to the planktonic cells.
23996303	4	15	theme	pneumoniae	648:657	arg1	matrix					627:632	capsular matrix	618:632	capsular matrix of Klebsiella pneumoniae	618:657	The challenge of using enzymes derived from unrelated bacteria for the degradation of capsular matrix of Klebsiella pneumoniae has not been dealt in the past.
23996303	0	16	theme	depolymerase	118:129	arg1	production					131:140	depolymerase production	118:140	depolymerase production	118:140	Aeromonas punctata derived depolymerase that disrupts the integrity of Klebsiella pneumoniae capsule: optimization of depolymerase production.
23996303	7	17	theme	capsular	1267:1274	arg1	polysaccharide					1276:1289	capsular polysaccharide	1267:1289	capsular polysaccharide	1267:1289	Out of the 19 variables, media composition giving maximum expression levels of the enzyme consisted of 1 mg ml(-1) galactose and ammonium chloride, 1.5 mg ml(-1) each of capsular polysaccharide (CPS) and magnesium sulfate.
23996303	5	18	theme	borne	911:915	arg1	enzyme					930:935	the available phage borne depolymerase enzyme	891:935	the available phage borne depolymerase enzyme	891:935	Thus, statistical optimization was done to enhance depolymerase production by Aeromonas punctata, directed against the exopolysaccharide matrix of Klebsiella pneumoniae B5055, capable of substituting the available phage borne depolymerase enzyme.
23996303	1	19	theme	biofilm	179:185	arg1	structures					187:196	dense, highly hydrated biofilm structures	156:196	dense, highly hydrated biofilm structures	156:196	Formation of dense, highly hydrated biofilm structures pose a risk for public and environmental health.
23996303	5	20	theme	capable	867:873	arg1	production					755:764	depolymerase production	742:764	depolymerase production	742:764	Thus, statistical optimization was done to enhance depolymerase production by Aeromonas punctata, directed against the exopolysaccharide matrix of Klebsiella pneumoniae B5055, capable of substituting the available phage borne depolymerase enzyme.
23996303	7	21	theme	maximum	1147:1153	arg1	levels					1166:1171	maximum expression levels	1147:1171	maximum expression levels of the enzyme	1147:1185	Out of the 19 variables, media composition giving maximum expression levels of the enzyme consisted of 1 mg ml(-1) galactose and ammonium chloride, 1.5 mg ml(-1) each of capsular polysaccharide (CPS) and magnesium sulfate.
23996303	8	22	theme	band	1368:1371	arg1	analysis					1336:1343	Tryptic peptide analysis	1320:1343	Tryptic peptide analysis of the purified 29 kDa band by Matrix assisted laser desorption ionization-time of flight (MALDI-TOF)	1320:1445	Tryptic peptide analysis of the purified 29 kDa band by Matrix assisted laser desorption ionization-time of flight (MALDI-TOF) showed a high homology with a protein of unknown function from Aeromonas cavaie Ae398.
23996303	1	23	theme	structures	187:196	arg1	Formation					143:151	Formation	143:151	Formation of dense, highly hydrated biofilm structures	143:196	Formation of dense, highly hydrated biofilm structures pose a risk for public and environmental health.
23996303	8	24	from	Ae398	1527:1531	arg1	protein					1477:1483	a protein	1475:1483	a protein of unknown function from Aeromonas cavaie Ae398	1475:1531	Tryptic peptide analysis of the purified 29 kDa band by Matrix assisted laser desorption ionization-time of flight (MALDI-TOF) showed a high homology with a protein of unknown function from Aeromonas cavaie Ae398.
23996303	6	25	theme	composite	963:971	arg1	CCD					981:983	CCD	981:983	CCD	981:983	Optimization via central composite design (CCD) resulted in 16-fold enhancement in depolymerase yield (166.65 µmoles ml(-1) min(-1) ) over unoptimized medium.
23996303	6	25	theme	composite	963:971	arg1	design					973:978	central composite design	955:978	central composite design (CCD)	955:984	Optimization via central composite design (CCD) resulted in 16-fold enhancement in depolymerase yield (166.65 µmoles ml(-1) min(-1) ) over unoptimized medium.
23996303	7	26	theme	magnesium	1301:1309	arg1	sulfate					1311:1317	magnesium sulfate	1301:1317	magnesium sulfate	1301:1317	Out of the 19 variables, media composition giving maximum expression levels of the enzyme consisted of 1 mg ml(-1) galactose and ammonium chloride, 1.5 mg ml(-1) each of capsular polysaccharide (CPS) and magnesium sulfate.
23996303	5	27	theme	exopolysaccharide	810:826	arg1	matrix					828:833	the exopolysaccharide matrix	806:833	the exopolysaccharide matrix of Klebsiella pneumoniae B5055	806:864	Thus, statistical optimization was done to enhance depolymerase production by Aeromonas punctata, directed against the exopolysaccharide matrix of Klebsiella pneumoniae B5055, capable of substituting the available phage borne depolymerase enzyme.
23996303	8	28	theme	assisted	1383:1390	arg1	ionization-time					1409:1423	Matrix assisted laser desorption ionization-time	1376:1423	Matrix assisted laser desorption ionization-time of flight (MALDI-TOF)	1376:1445	Tryptic peptide analysis of the purified 29 kDa band by Matrix assisted laser desorption ionization-time of flight (MALDI-TOF) showed a high homology with a protein of unknown function from Aeromonas cavaie Ae398.
23996303	0	29	theme	Aeromonas	0:8	arg1	punctata					10:17	Aeromonas punctata	0:17	Aeromonas punctata	0:17	Aeromonas punctata derived depolymerase that disrupts the integrity of Klebsiella pneumoniae capsule: optimization of depolymerase production.
23996303	8	30	theme	unknown	1488:1494	arg1	function					1496:1503	unknown function	1488:1503	unknown function	1488:1503	Tryptic peptide analysis of the purified 29 kDa band by Matrix assisted laser desorption ionization-time of flight (MALDI-TOF) showed a high homology with a protein of unknown function from Aeromonas cavaie Ae398.
23996303	8	31	theme	Matrix	1376:1381	arg1	ionization-time					1409:1423	Matrix assisted laser desorption ionization-time	1376:1423	Matrix assisted laser desorption ionization-time of flight (MALDI-TOF)	1376:1445	Tryptic peptide analysis of the purified 29 kDa band by Matrix assisted laser desorption ionization-time of flight (MALDI-TOF) showed a high homology with a protein of unknown function from Aeromonas cavaie Ae398.
23996303	8	32	theme	high	1456:1459	arg1	homology					1461:1468	a high homology	1454:1468	a high homology with a protein of unknown function from Aeromonas cavaie Ae398	1454:1531	Tryptic peptide analysis of the purified 29 kDa band by Matrix assisted laser desorption ionization-time of flight (MALDI-TOF) showed a high homology with a protein of unknown function from Aeromonas cavaie Ae398.
23996303	7	33	dep	1.5 mg ml	1245:1253	arg1	each					1259:1262	each	1259:1262	each	1259:1262	Out of the 19 variables, media composition giving maximum expression levels of the enzyme consisted of 1 mg ml(-1) galactose and ammonium chloride, 1.5 mg ml(-1) each of capsular polysaccharide (CPS) and magnesium sulfate.
23996303	7	33	dep	1.5 mg ml	1245:1253	arg1	polysaccharide					1276:1289	capsular polysaccharide	1267:1289	capsular polysaccharide	1267:1289	Out of the 19 variables, media composition giving maximum expression levels of the enzyme consisted of 1 mg ml(-1) galactose and ammonium chloride, 1.5 mg ml(-1) each of capsular polysaccharide (CPS) and magnesium sulfate.
23996303	1	34	dep	dense	156:160	arg1	hydrated					170:177	hydrated	170:177	hydrated	170:177	Formation of dense, highly hydrated biofilm structures pose a risk for public and environmental health.
23996303	8	35	theme	Aeromonas	1510:1518	arg1	Ae398					1527:1531	Aeromonas cavaie Ae398	1510:1531	Aeromonas cavaie Ae398	1510:1531	Tryptic peptide analysis of the purified 29 kDa band by Matrix assisted laser desorption ionization-time of flight (MALDI-TOF) showed a high homology with a protein of unknown function from Aeromonas cavaie Ae398.
23996303	3	36	theme	immune	517:522	arg1	system					524:529	immune system	517:529	immune system	517:529	Using enzymes as anti-biofouling agents, will improve penetration of antimicrobials and increase susceptibility of biofilms to components of immune system.
23996303	5	37	theme	available	895:903	arg1	enzyme					930:935	the available phage borne depolymerase enzyme	891:935	the available phage borne depolymerase enzyme	891:935	Thus, statistical optimization was done to enhance depolymerase production by Aeromonas punctata, directed against the exopolysaccharide matrix of Klebsiella pneumoniae B5055, capable of substituting the available phage borne depolymerase enzyme.
23996303	4	38	theme	matrix	627:632	arg1	degradation					603:613	the degradation	599:613	the degradation of capsular matrix of Klebsiella pneumoniae	599:657	The challenge of using enzymes derived from unrelated bacteria for the degradation of capsular matrix of Klebsiella pneumoniae has not been dealt in the past.
23996303	0	39	theme	production	131:140	arg1	optimization					102:113	optimization	102:113	optimization of depolymerase production	102:140	Aeromonas punctata derived depolymerase that disrupts the integrity of Klebsiella pneumoniae capsule: optimization of depolymerase production.
23996303	6	40	theme	central	955:961	arg1	CCD					981:983	CCD	981:983	CCD	981:983	Optimization via central composite design (CCD) resulted in 16-fold enhancement in depolymerase yield (166.65 µmoles ml(-1) min(-1) ) over unoptimized medium.
23996303	6	40	theme	central	955:961	arg1	design					973:978	central composite design	955:978	central composite design (CCD)	955:984	Optimization via central composite design (CCD) resulted in 16-fold enhancement in depolymerase yield (166.65 µmoles ml(-1) min(-1) ) over unoptimized medium.
23996303	8	41	theme	laser	1392:1396	arg1	ionization-time					1409:1423	Matrix assisted laser desorption ionization-time	1376:1423	Matrix assisted laser desorption ionization-time of flight (MALDI-TOF)	1376:1445	Tryptic peptide analysis of the purified 29 kDa band by Matrix assisted laser desorption ionization-time of flight (MALDI-TOF) showed a high homology with a protein of unknown function from Aeromonas cavaie Ae398.
23996303	3	42	theme	system	524:529	arg1	components					503:512	components	503:512	components of immune system	503:529	Using enzymes as anti-biofouling agents, will improve penetration of antimicrobials and increase susceptibility of biofilms to components of immune system.
23996303	5	43	theme	pneumoniae	849:858	arg1	matrix					828:833	the exopolysaccharide matrix	806:833	the exopolysaccharide matrix of Klebsiella pneumoniae B5055	806:864	Thus, statistical optimization was done to enhance depolymerase production by Aeromonas punctata, directed against the exopolysaccharide matrix of Klebsiella pneumoniae B5055, capable of substituting the available phage borne depolymerase enzyme.
23996303	4	44	theme	capsular	618:625	arg1	matrix					627:632	capsular matrix	618:632	capsular matrix of Klebsiella pneumoniae	618:657	The challenge of using enzymes derived from unrelated bacteria for the degradation of capsular matrix of Klebsiella pneumoniae has not been dealt in the past.
23996303	5	45	theme	Aeromonas	769:777	arg1	punctata					779:786	Aeromonas punctata	769:786	Aeromonas punctata	769:786	Thus, statistical optimization was done to enhance depolymerase production by Aeromonas punctata, directed against the exopolysaccharide matrix of Klebsiella pneumoniae B5055, capable of substituting the available phage borne depolymerase enzyme.
23996303	5	46	theme	depolymerase	917:928	arg1	enzyme					930:935	the available phage borne depolymerase enzyme	891:935	the available phage borne depolymerase enzyme	891:935	Thus, statistical optimization was done to enhance depolymerase production by Aeromonas punctata, directed against the exopolysaccharide matrix of Klebsiella pneumoniae B5055, capable of substituting the available phage borne depolymerase enzyme.
23996303	2	47	theme	Extracellular	247:259	arg1	substances					271:280	Extracellular polymeric substances	247:280	Extracellular polymeric substances encompassing biofilms	247:302	Extracellular polymeric substances encompassing biofilms offer 1000-fold greater resistance as compared to the planktonic cells.
23996303	1	48	theme	public	214:219	arg1	health					239:244	public and environmental health	214:244	public and environmental health	214:244	Formation of dense, highly hydrated biofilm structures pose a risk for public and environmental health.
23996303	3	49	theme	biofilms	491:498	arg1	susceptibility					473:486	susceptibility	473:486	susceptibility of biofilms to components of immune system	473:529	Using enzymes as anti-biofouling agents, will improve penetration of antimicrobials and increase susceptibility of biofilms to components of immune system.
23996303	7	50	theme	1 mg ml	1200:1206	arg1	galactose					1212:1220	1 mg ml(-1) galactose	1200:1220	1 mg ml(-1) galactose	1200:1220	Out of the 19 variables, media composition giving maximum expression levels of the enzyme consisted of 1 mg ml(-1) galactose and ammonium chloride, 1.5 mg ml(-1) each of capsular polysaccharide (CPS) and magnesium sulfate.
23996303	9	51	theme	prophylactic	1611:1622	arg1	agent					1645:1649	prophylactic and/or a therapeutic agent	1611:1649	prophylactic and/or a therapeutic agent	1611:1649	Further improvements in the enzyme can lead to its successful development as prophylactic and/or a therapeutic agent.
23996303	7	52	theme	ammonium	1226:1233	arg1	chloride					1235:1242	ammonium chloride	1226:1242	ammonium chloride	1226:1242	Out of the 19 variables, media composition giving maximum expression levels of the enzyme consisted of 1 mg ml(-1) galactose and ammonium chloride, 1.5 mg ml(-1) each of capsular polysaccharide (CPS) and magnesium sulfate.
23996303	7	53	theme	enzyme	1180:1185	arg1	levels					1166:1171	maximum expression levels	1147:1171	maximum expression levels of the enzyme	1147:1185	Out of the 19 variables, media composition giving maximum expression levels of the enzyme consisted of 1 mg ml(-1) galactose and ammonium chloride, 1.5 mg ml(-1) each of capsular polysaccharide (CPS) and magnesium sulfate.
23996303	4	54	theme	unrelated	576:584	arg1	bacteria					586:593	unrelated bacteria	576:593	unrelated bacteria	576:593	The challenge of using enzymes derived from unrelated bacteria for the degradation of capsular matrix of Klebsiella pneumoniae has not been dealt in the past.
23996303	7	55	theme	media	1122:1126	arg1	composition					1128:1138	media composition	1122:1138	media composition giving maximum expression levels of the enzyme	1122:1185	Out of the 19 variables, media composition giving maximum expression levels of the enzyme consisted of 1 mg ml(-1) galactose and ammonium chloride, 1.5 mg ml(-1) each of capsular polysaccharide (CPS) and magnesium sulfate.
23996303	8	56	theme	peptide	1328:1334	arg1	analysis					1336:1343	Tryptic peptide analysis	1320:1343	Tryptic peptide analysis of the purified 29 kDa band by Matrix assisted laser desorption ionization-time of flight (MALDI-TOF)	1320:1445	Tryptic peptide analysis of the purified 29 kDa band by Matrix assisted laser desorption ionization-time of flight (MALDI-TOF) showed a high homology with a protein of unknown function from Aeromonas cavaie Ae398.
23996303	3	57	theme	antimicrobials	445:458	arg1	penetration					430:440	penetration	430:440	penetration of antimicrobials	430:458	Using enzymes as anti-biofouling agents, will improve penetration of antimicrobials and increase susceptibility of biofilms to components of immune system.
23996303	6	58	from	enhancement	1006:1016	arg1	yield					1034:1038	depolymerase yield	1021:1038	depolymerase yield	1021:1038	Optimization via central composite design (CCD) resulted in 16-fold enhancement in depolymerase yield (166.65 µmoles ml(-1) min(-1) ) over unoptimized medium.
23996303	8	59	theme	flight	1428:1433	arg1	ionization-time					1409:1423	Matrix assisted laser desorption ionization-time	1376:1423	Matrix assisted laser desorption ionization-time of flight (MALDI-TOF)	1376:1445	Tryptic peptide analysis of the purified 29 kDa band by Matrix assisted laser desorption ionization-time of flight (MALDI-TOF) showed a high homology with a protein of unknown function from Aeromonas cavaie Ae398.
23996303	7	60	theme	expression	1155:1164	arg1	levels					1166:1171	maximum expression levels	1147:1171	maximum expression levels of the enzyme	1147:1185	Out of the 19 variables, media composition giving maximum expression levels of the enzyme consisted of 1 mg ml(-1) galactose and ammonium chloride, 1.5 mg ml(-1) each of capsular polysaccharide (CPS) and magnesium sulfate.
23996303	9	61	theme	therapeutic	1633:1643	arg1	agent					1645:1649	prophylactic and/or a therapeutic agent	1611:1649	prophylactic and/or a therapeutic agent	1611:1649	Further improvements in the enzyme can lead to its successful development as prophylactic and/or a therapeutic agent.
23996303	9	62	theme	Further	1534:1540	arg1	improvements					1542:1553	Further improvements	1534:1553	Further improvements in the enzyme	1534:1567	Further improvements in the enzyme can lead to its successful development as prophylactic and/or a therapeutic agent.
23996303	4	63	attach	derived	563:569	arg2	enzymes					555:561	enzymes	555:561	enzymes derived from unrelated bacteria for the degradation of capsular matrix of Klebsiella pneumoniae	555:657	The challenge of using enzymes derived from unrelated bacteria for the degradation of capsular matrix of Klebsiella pneumoniae has not been dealt in the past.
23996303	4	63	attach	derived	563:569	arg1	bacteria					586:593	unrelated bacteria	576:593	unrelated bacteria	576:593	The challenge of using enzymes derived from unrelated bacteria for the degradation of capsular matrix of Klebsiella pneumoniae has not been dealt in the past.
23996303	0	64	dep	Klebsiella	71:80	arg1	pneumoniae					82:91	pneumoniae	82:91	pneumoniae	82:91	Aeromonas punctata derived depolymerase that disrupts the integrity of Klebsiella pneumoniae capsule: optimization of depolymerase production.
23996303	1	65	theme	environmental	225:237	arg1	health					239:244	public and environmental health	214:244	public and environmental health	214:244	Formation of dense, highly hydrated biofilm structures pose a risk for public and environmental health.
23996303	5	66	theme	depolymerase	742:753	arg1	production					755:764	depolymerase production	742:764	depolymerase production	742:764	Thus, statistical optimization was done to enhance depolymerase production by Aeromonas punctata, directed against the exopolysaccharide matrix of Klebsiella pneumoniae B5055, capable of substituting the available phage borne depolymerase enzyme.
23996303	8	67	theme	function	1496:1503	arg1	protein					1477:1483	a protein	1475:1483	a protein of unknown function from Aeromonas cavaie Ae398	1475:1531	Tryptic peptide analysis of the purified 29 kDa band by Matrix assisted laser desorption ionization-time of flight (MALDI-TOF) showed a high homology with a protein of unknown function from Aeromonas cavaie Ae398.
23996303	0	68	dep	derived	19:25	arg1	optimization					102:113	optimization	102:113	optimization of depolymerase production	102:140	Aeromonas punctata derived depolymerase that disrupts the integrity of Klebsiella pneumoniae capsule: optimization of depolymerase production.
23996303	2	69	theme	planktonic	358:367	arg1	cells					369:373	the planktonic cells	354:373	the planktonic cells	354:373	Extracellular polymeric substances encompassing biofilms offer 1000-fold greater resistance as compared to the planktonic cells.
23996303	8	70	theme	Tryptic	1320:1326	arg1	analysis					1336:1343	Tryptic peptide analysis	1320:1343	Tryptic peptide analysis of the purified 29 kDa band by Matrix assisted laser desorption ionization-time of flight (MALDI-TOF)	1320:1445	Tryptic peptide analysis of the purified 29 kDa band by Matrix assisted laser desorption ionization-time of flight (MALDI-TOF) showed a high homology with a protein of unknown function from Aeromonas cavaie Ae398.
23996303	0	71	theme	Klebsiella	71:80	arg1	capsule					93:99	Klebsiella pneumoniae capsule	71:99	Klebsiella pneumoniae capsule	71:99	Aeromonas punctata derived depolymerase that disrupts the integrity of Klebsiella pneumoniae capsule: optimization of depolymerase production.
23996303	8	72	theme	desorption	1398:1407	arg1	ionization-time					1409:1423	Matrix assisted laser desorption ionization-time	1376:1423	Matrix assisted laser desorption ionization-time of flight (MALDI-TOF)	1376:1445	Tryptic peptide analysis of the purified 29 kDa band by Matrix assisted laser desorption ionization-time of flight (MALDI-TOF) showed a high homology with a protein of unknown function from Aeromonas cavaie Ae398.
23996303	5	73	theme	phage	905:909	arg1	enzyme					930:935	the available phage borne depolymerase enzyme	891:935	the available phage borne depolymerase enzyme	891:935	Thus, statistical optimization was done to enhance depolymerase production by Aeromonas punctata, directed against the exopolysaccharide matrix of Klebsiella pneumoniae B5055, capable of substituting the available phage borne depolymerase enzyme.
23996303	1	74	theme	dense	156:160	arg1	structures					187:196	dense, highly hydrated biofilm structures	156:196	dense, highly hydrated biofilm structures	156:196	Formation of dense, highly hydrated biofilm structures pose a risk for public and environmental health.
23996303	6	75	theme	16-fold	998:1004	arg1	enhancement					1006:1016	16-fold enhancement	998:1016	16-fold enhancement in depolymerase yield	998:1038	Optimization via central composite design (CCD) resulted in 16-fold enhancement in depolymerase yield (166.65 µmoles ml(-1) min(-1) ) over unoptimized medium.
23996303	2	76	theme	polymeric	261:269	arg1	substances					271:280	Extracellular polymeric substances	247:280	Extracellular polymeric substances encompassing biofilms	247:302	Extracellular polymeric substances encompassing biofilms offer 1000-fold greater resistance as compared to the planktonic cells.
23996303	6	77	theme	unoptimized	1077:1087	arg1	medium					1089:1094	unoptimized medium	1077:1094	unoptimized medium	1077:1094	Optimization via central composite design (CCD) resulted in 16-fold enhancement in depolymerase yield (166.65 µmoles ml(-1) min(-1) ) over unoptimized medium.
23996303	6	78	theme	depolymerase	1021:1032	arg1	yield					1034:1038	depolymerase yield	1021:1038	depolymerase yield	1021:1038	Optimization via central composite design (CCD) resulted in 16-fold enhancement in depolymerase yield (166.65 µmoles ml(-1) min(-1) ) over unoptimized medium.
28972729	0	0	theme	Delivery	115:122	arg1	Carriers					124:131	Sustained Tetracycline Hydrochloride Delivery Carriers	78:131	Sustained Tetracycline Hydrochloride Delivery Carriers	78:131	Autotemplate Microcapsules of CaCO3/Pectin and Nonstoichiometric Complexes as Sustained Tetracycline Hydrochloride Delivery Carriers.
28972729	6	1	theme	release	969:975	arg1	mechanism					977:985	the drug release mechanism	960:985	the drug release mechanism	960:985	The kinetics of the drug release mechanism was followed using Higuchi and Korsmeyer-Peppas mathematical models.
28972729	1	2	theme	autotemplate	178:189	arg1	method					191:196	an autotemplate method	175:196	an autotemplate method for assembling hollow CaCO3 capsules by using pH-sensitive polymers	175:264	New types of composites were obtained by an autotemplate method for assembling hollow CaCO3 capsules by using pH-sensitive polymers.
28972729	2	3	theme	crystal	507:513	arg1	growth					515:520	the crystal growth	503:520	the crystal growth	503:520	Five pectin samples, which differ in the methylation degree and/or amide content, and some nonstoichiometric polyelectrolyte complex dispersions, prepared with the pectin samples and poly(allylamine hydrochloride), were used to control the crystal growth.
28972729	6	4	theme	drug	964:967	arg1	mechanism					977:985	the drug release mechanism	960:985	the drug release mechanism	960:985	The kinetics of the drug release mechanism was followed using Higuchi and Korsmeyer-Peppas mathematical models.
28972729	2	5	theme	complex	392:398	arg1	dispersions					400:410	some nonstoichiometric polyelectrolyte complex dispersions	353:410	some nonstoichiometric polyelectrolyte complex dispersions	353:410	Five pectin samples, which differ in the methylation degree and/or amide content, and some nonstoichiometric polyelectrolyte complex dispersions, prepared with the pectin samples and poly(allylamine hydrochloride), were used to control the crystal growth.
28972729	3	6	theme	scanning	576:583	arg1	microscopy					594:603	scanning electron microscopy	576:603	scanning electron microscopy	576:603	The morphology of the composites was investigated by scanning electron microscopy, and the polymorphs characteristics were investigated by FTIR spectroscopy.
28972729	2	7	theme	methylation	308:318	arg1	degree					320:325	the methylation degree	304:325	the methylation degree	304:325	Five pectin samples, which differ in the methylation degree and/or amide content, and some nonstoichiometric polyelectrolyte complex dispersions, prepared with the pectin samples and poly(allylamine hydrochloride), were used to control the crystal growth.
28972729	4	8	theme	X-ray	753:757	arg1	spectroscopy					773:784	X-ray photoelectron spectroscopy	753:784	X-ray photoelectron spectroscopy	753:784	The presence of the polymer in the composite particles was evidenced by X-ray photoelectron spectroscopy, particle charge density, and zeta-potential.
28972729	5	9	theme	hydrochloride	921:933	arg1	carrier					935:941	a tetracycline hydrochloride carrier	906:941	a tetracycline hydrochloride carrier	906:941	The new CaCO3/pectin hollow capsules were tested as a possible matrix for a tetracycline hydrochloride carrier.
28972729	4	10	theme	polymer	701:707	arg1	presence					685:692	The presence	681:692	The presence of the polymer in the composite particles	681:734	The presence of the polymer in the composite particles was evidenced by X-ray photoelectron spectroscopy, particle charge density, and zeta-potential.
28972729	2	11	theme	pectin	431:436	arg1	samples					438:444	the pectin samples	427:444	the pectin samples	427:444	Five pectin samples, which differ in the methylation degree and/or amide content, and some nonstoichiometric polyelectrolyte complex dispersions, prepared with the pectin samples and poly(allylamine hydrochloride), were used to control the crystal growth.
28972729	0	12	theme	Hydrochloride	101:113	arg1	Carriers					124:131	Sustained Tetracycline Hydrochloride Delivery Carriers	78:131	Sustained Tetracycline Hydrochloride Delivery Carriers	78:131	Autotemplate Microcapsules of CaCO3/Pectin and Nonstoichiometric Complexes as Sustained Tetracycline Hydrochloride Delivery Carriers.
28972729	4	13	theme	particle	787:794	arg1	density					803:809	particle charge density	787:809	particle charge density	787:809	The presence of the polymer in the composite particles was evidenced by X-ray photoelectron spectroscopy, particle charge density, and zeta-potential.
28972729	1	14	theme	hollow	213:218	arg1	capsules					226:233	hollow CaCO3 capsules	213:233	hollow CaCO3 capsules	213:233	New types of composites were obtained by an autotemplate method for assembling hollow CaCO3 capsules by using pH-sensitive polymers.
28972729	0	15	theme	Autotemplate	0:11	arg1	Microcapsules					13:25	Autotemplate Microcapsules	0:25	Autotemplate Microcapsules of CaCO3/Pectin and Nonstoichiometric	0:63	Autotemplate Microcapsules of CaCO3/Pectin and Nonstoichiometric Complexes as Sustained Tetracycline Hydrochloride Delivery Carriers.
28972729	1	16	theme	CaCO3	220:224	arg1	capsules					226:233	hollow CaCO3 capsules	213:233	hollow CaCO3 capsules	213:233	New types of composites were obtained by an autotemplate method for assembling hollow CaCO3 capsules by using pH-sensitive polymers.
28972729	0	17	theme	CaCO3/Pectin	30:41	arg1	Microcapsules					13:25	Autotemplate Microcapsules	0:25	Autotemplate Microcapsules of CaCO3/Pectin and Nonstoichiometric	0:63	Autotemplate Microcapsules of CaCO3/Pectin and Nonstoichiometric Complexes as Sustained Tetracycline Hydrochloride Delivery Carriers.
28972729	6	18	theme	mathematical	1035:1046	arg1	models					1048:1053	Higuchi and Korsmeyer-Peppas mathematical models	1006:1053	Higuchi and Korsmeyer-Peppas mathematical models	1006:1053	The kinetics of the drug release mechanism was followed using Higuchi and Korsmeyer-Peppas mathematical models.
28972729	2	19	theme	amide	334:338	arg1	content					340:346	amide content	334:346	amide content	334:346	Five pectin samples, which differ in the methylation degree and/or amide content, and some nonstoichiometric polyelectrolyte complex dispersions, prepared with the pectin samples and poly(allylamine hydrochloride), were used to control the crystal growth.
28972729	4	20	from	presence	685:692	arg1	particles					726:734	the composite particles	712:734	the composite particles	712:734	The presence of the polymer in the composite particles was evidenced by X-ray photoelectron spectroscopy, particle charge density, and zeta-potential.
28972729	2	21	theme	allylamine	455:464	arg1	poly					450:453	poly	450:453	poly(allylamine hydrochloride)	450:479	Five pectin samples, which differ in the methylation degree and/or amide content, and some nonstoichiometric polyelectrolyte complex dispersions, prepared with the pectin samples and poly(allylamine hydrochloride), were used to control the crystal growth.
28972729	2	21	theme	allylamine	455:464	arg1	hydrochloride					466:478	allylamine hydrochloride	455:478	allylamine hydrochloride	455:478	Five pectin samples, which differ in the methylation degree and/or amide content, and some nonstoichiometric polyelectrolyte complex dispersions, prepared with the pectin samples and poly(allylamine hydrochloride), were used to control the crystal growth.
28972729	6	22	theme	Korsmeyer-Peppas	1018:1033	arg1	models					1048:1053	Higuchi and Korsmeyer-Peppas mathematical models	1006:1053	Higuchi and Korsmeyer-Peppas mathematical models	1006:1053	The kinetics of the drug release mechanism was followed using Higuchi and Korsmeyer-Peppas mathematical models.
28972729	4	23	theme	charge	796:801	arg1	density					803:809	particle charge density	787:809	particle charge density	787:809	The presence of the polymer in the composite particles was evidenced by X-ray photoelectron spectroscopy, particle charge density, and zeta-potential.
28972729	5	24	theme	possible	886:893	arg1	matrix					895:900	a possible matrix	884:900	a possible matrix for a tetracycline hydrochloride carrier	884:941	The new CaCO3/pectin hollow capsules were tested as a possible matrix for a tetracycline hydrochloride carrier.
28972729	5	24	theme	possible	886:893	arg1	capsules					860:867	The new CaCO3/pectin hollow capsules	832:867	The new CaCO3/pectin hollow capsules	832:867	The new CaCO3/pectin hollow capsules were tested as a possible matrix for a tetracycline hydrochloride carrier.
28972729	0	25	theme	Nonstoichiometric	47:63	arg1	Microcapsules					13:25	Autotemplate Microcapsules	0:25	Autotemplate Microcapsules of CaCO3/Pectin and Nonstoichiometric	0:63	Autotemplate Microcapsules of CaCO3/Pectin and Nonstoichiometric Complexes as Sustained Tetracycline Hydrochloride Delivery Carriers.
28972729	2	26	theme	nonstoichiometric	358:374	arg1	dispersions					400:410	some nonstoichiometric polyelectrolyte complex dispersions	353:410	some nonstoichiometric polyelectrolyte complex dispersions	353:410	Five pectin samples, which differ in the methylation degree and/or amide content, and some nonstoichiometric polyelectrolyte complex dispersions, prepared with the pectin samples and poly(allylamine hydrochloride), were used to control the crystal growth.
28972729	2	27	theme	pectin	272:277	arg1	samples					279:285	Five pectin samples	267:285	Five pectin samples	267:285	Five pectin samples, which differ in the methylation degree and/or amide content, and some nonstoichiometric polyelectrolyte complex dispersions, prepared with the pectin samples and poly(allylamine hydrochloride), were used to control the crystal growth.
28972729	5	28	theme	new	836:838	arg1	matrix					895:900	a possible matrix	884:900	a possible matrix for a tetracycline hydrochloride carrier	884:941	The new CaCO3/pectin hollow capsules were tested as a possible matrix for a tetracycline hydrochloride carrier.
28972729	5	28	theme	new	836:838	arg1	capsules					860:867	The new CaCO3/pectin hollow capsules	832:867	The new CaCO3/pectin hollow capsules	832:867	The new CaCO3/pectin hollow capsules were tested as a possible matrix for a tetracycline hydrochloride carrier.
28972729	6	29	theme	Higuchi	1006:1012	arg1	models					1048:1053	Higuchi and Korsmeyer-Peppas mathematical models	1006:1053	Higuchi and Korsmeyer-Peppas mathematical models	1006:1053	The kinetics of the drug release mechanism was followed using Higuchi and Korsmeyer-Peppas mathematical models.
28972729	1	30	theme	pH-sensitive	244:255	arg1	polymers					257:264	pH-sensitive polymers	244:264	pH-sensitive polymers	244:264	New types of composites were obtained by an autotemplate method for assembling hollow CaCO3 capsules by using pH-sensitive polymers.
28972729	5	31	theme	CaCO3/pectin	840:851	arg1	matrix					895:900	a possible matrix	884:900	a possible matrix for a tetracycline hydrochloride carrier	884:941	The new CaCO3/pectin hollow capsules were tested as a possible matrix for a tetracycline hydrochloride carrier.
28972729	5	31	theme	CaCO3/pectin	840:851	arg1	capsules					860:867	The new CaCO3/pectin hollow capsules	832:867	The new CaCO3/pectin hollow capsules	832:867	The new CaCO3/pectin hollow capsules were tested as a possible matrix for a tetracycline hydrochloride carrier.
28972729	2	32	theme	polyelectrolyte	376:390	arg1	dispersions					400:410	some nonstoichiometric polyelectrolyte complex dispersions	353:410	some nonstoichiometric polyelectrolyte complex dispersions	353:410	Five pectin samples, which differ in the methylation degree and/or amide content, and some nonstoichiometric polyelectrolyte complex dispersions, prepared with the pectin samples and poly(allylamine hydrochloride), were used to control the crystal growth.
28972729	5	33	theme	hollow	853:858	arg1	matrix					895:900	a possible matrix	884:900	a possible matrix for a tetracycline hydrochloride carrier	884:941	The new CaCO3/pectin hollow capsules were tested as a possible matrix for a tetracycline hydrochloride carrier.
28972729	5	33	theme	hollow	853:858	arg1	capsules					860:867	The new CaCO3/pectin hollow capsules	832:867	The new CaCO3/pectin hollow capsules	832:867	The new CaCO3/pectin hollow capsules were tested as a possible matrix for a tetracycline hydrochloride carrier.
28972729	3	34	theme	electron	585:592	arg1	microscopy					594:603	scanning electron microscopy	576:603	scanning electron microscopy	576:603	The morphology of the composites was investigated by scanning electron microscopy, and the polymorphs characteristics were investigated by FTIR spectroscopy.
28972729	6	35	theme	mechanism	977:985	arg1	kinetics					948:955	The kinetics	944:955	The kinetics of the drug release mechanism	944:985	The kinetics of the drug release mechanism was followed using Higuchi and Korsmeyer-Peppas mathematical models.
28972729	3	36	theme	polymorphs	614:623	arg1	characteristics					625:639	the polymorphs characteristics	610:639	the polymorphs characteristics	610:639	The morphology of the composites was investigated by scanning electron microscopy, and the polymorphs characteristics were investigated by FTIR spectroscopy.
28972729	1	37	theme	composites	147:156	arg1	types					138:142	New types	134:142	New types of composites	134:156	New types of composites were obtained by an autotemplate method for assembling hollow CaCO3 capsules by using pH-sensitive polymers.
28972729	3	38	theme	FTIR	662:665	arg1	spectroscopy					667:678	FTIR spectroscopy	662:678	FTIR spectroscopy	662:678	The morphology of the composites was investigated by scanning electron microscopy, and the polymorphs characteristics were investigated by FTIR spectroscopy.
28972729	4	39	theme	composite	716:724	arg1	particles					726:734	the composite particles	712:734	the composite particles	712:734	The presence of the polymer in the composite particles was evidenced by X-ray photoelectron spectroscopy, particle charge density, and zeta-potential.
28972729	1	40	theme	New	134:136	arg1	types					138:142	New types	134:142	New types of composites	134:156	New types of composites were obtained by an autotemplate method for assembling hollow CaCO3 capsules by using pH-sensitive polymers.
28972729	4	41	attach	presence	685:692	arg2	polymer					701:707	the polymer	697:707	the polymer	697:707	The presence of the polymer in the composite particles was evidenced by X-ray photoelectron spectroscopy, particle charge density, and zeta-potential.
28972729	4	41	attach	presence	685:692	arg1	particles					726:734	the composite particles	712:734	the composite particles	712:734	The presence of the polymer in the composite particles was evidenced by X-ray photoelectron spectroscopy, particle charge density, and zeta-potential.
28972729	4	42	theme	photoelectron	759:771	arg1	spectroscopy					773:784	X-ray photoelectron spectroscopy	753:784	X-ray photoelectron spectroscopy	753:784	The presence of the polymer in the composite particles was evidenced by X-ray photoelectron spectroscopy, particle charge density, and zeta-potential.
28972729	0	43	theme	Tetracycline	88:99	arg1	Carriers					124:131	Sustained Tetracycline Hydrochloride Delivery Carriers	78:131	Sustained Tetracycline Hydrochloride Delivery Carriers	78:131	Autotemplate Microcapsules of CaCO3/Pectin and Nonstoichiometric Complexes as Sustained Tetracycline Hydrochloride Delivery Carriers.
28972729	5	44	theme	tetracycline	908:919	arg1	carrier					935:941	a tetracycline hydrochloride carrier	906:941	a tetracycline hydrochloride carrier	906:941	The new CaCO3/pectin hollow capsules were tested as a possible matrix for a tetracycline hydrochloride carrier.
28972729	0	45	theme	Sustained	78:86	arg1	Carriers					124:131	Sustained Tetracycline Hydrochloride Delivery Carriers	78:131	Sustained Tetracycline Hydrochloride Delivery Carriers	78:131	Autotemplate Microcapsules of CaCO3/Pectin and Nonstoichiometric Complexes as Sustained Tetracycline Hydrochloride Delivery Carriers.
28972729	2	46	used	used	487:490	arg2	dispersions					400:410	some nonstoichiometric polyelectrolyte complex dispersions	353:410	some nonstoichiometric polyelectrolyte complex dispersions	353:410	Five pectin samples, which differ in the methylation degree and/or amide content, and some nonstoichiometric polyelectrolyte complex dispersions, prepared with the pectin samples and poly(allylamine hydrochloride), were used to control the crystal growth.
28972729	2	46	used	used	487:490	arg2	samples					279:285	Five pectin samples	267:285	Five pectin samples	267:285	Five pectin samples, which differ in the methylation degree and/or amide content, and some nonstoichiometric polyelectrolyte complex dispersions, prepared with the pectin samples and poly(allylamine hydrochloride), were used to control the crystal growth.
28972729	3	47	theme	composites	545:554	arg1	morphology					527:536	The morphology	523:536	The morphology of the composites	523:554	The morphology of the composites was investigated by scanning electron microscopy, and the polymorphs characteristics were investigated by FTIR spectroscopy.
24382476	1	0	theme	biological	143:152	arg1	treatment					165:173	biological wastewater treatment	143:173	biological wastewater treatment	143:173	The sludge floc stability is essential for the solid/liquid separation in biological wastewater treatment.
24382476	1	1	theme	wastewater	154:163	arg1	treatment					165:173	biological wastewater treatment	143:173	biological wastewater treatment	143:173	The sludge floc stability is essential for the solid/liquid separation in biological wastewater treatment.
24382476	0	2	from	Influences	0:9	arg1	stability					32:40	the stability	28:40	the stability of activated sludge flocs	28:66	Influences of D-tyrosine on the stability of activated sludge flocs.
24382476	5	3	theme	d-tyrosine	594:603	arg1	content					605:611	d-tyrosine content	594:611	d-tyrosine content	594:611	A high correlation coefficient was observed between the composition of EPS fraction and d-tyrosine content.
24382476	7	4	theme	inhibited	890:898	arg1	production					904:913	the inhibited EPS production	886:913	the inhibited EPS production	886:913	A linear relationship between the extracellular polymeric substances and surface characteristics for sludge flocs indicated that the inhibited EPS production may be responsible for the instability of sludge upon the addition of d-tyrosine.
24382476	7	4	theme	inhibited	890:898	arg1	responsible					922:932	responsible	922:932	responsible	922:932	A linear relationship between the extracellular polymeric substances and surface characteristics for sludge flocs indicated that the inhibited EPS production may be responsible for the instability of sludge upon the addition of d-tyrosine.
24382476	1	5	from	separation	129:138	arg1	treatment					165:173	biological wastewater treatment	143:173	biological wastewater treatment	143:173	The sludge floc stability is essential for the solid/liquid separation in biological wastewater treatment.
24382476	5	6	theme	content	605:611	arg1	composition					562:572	the composition	558:572	the composition of EPS fraction and d-tyrosine content	558:611	A high correlation coefficient was observed between the composition of EPS fraction and d-tyrosine content.
24382476	2	7	from	effect	195:200	arg1	characteristics					264:278	surface characteristics	256:278	surface characteristics	256:278	In this study, the effect of an exogenous d-tyrosine on the shear stability and surface characteristics of activated sludge flocs was investigated.
24382476	2	7	from	effect	195:200	arg1	stability					242:250	shear stability	236:250	shear stability	236:250	In this study, the effect of an exogenous d-tyrosine on the shear stability and surface characteristics of activated sludge flocs was investigated.
24382476	3	8	from	stable	359:364	arg1	addition					373:380	the addition	369:380	the addition of d-tyrosine	369:394	Sludge flocs were found to be less stable in the addition of d-tyrosine.
24382476	2	9	theme	surface	256:262	arg1	characteristics					264:278	surface characteristics	256:278	surface characteristics	256:278	In this study, the effect of an exogenous d-tyrosine on the shear stability and surface characteristics of activated sludge flocs was investigated.
24382476	7	10	theme	sludge	858:863	arg1	flocs					865:869	sludge flocs	858:869	sludge flocs	858:869	A linear relationship between the extracellular polymeric substances and surface characteristics for sludge flocs indicated that the inhibited EPS production may be responsible for the instability of sludge upon the addition of d-tyrosine.
24382476	0	11	theme	flocs	62:66	arg1	stability					32:40	the stability	28:40	the stability of activated sludge flocs	28:66	Influences of D-tyrosine on the stability of activated sludge flocs.
24382476	1	12	theme	sludge	73:78	arg1	essential					98:106	essential	98:106	essential	98:106	The sludge floc stability is essential for the solid/liquid separation in biological wastewater treatment.
24382476	1	12	theme	sludge	73:78	arg1	stability					85:93	The sludge floc stability	69:93	The sludge floc stability	69:93	The sludge floc stability is essential for the solid/liquid separation in biological wastewater treatment.
24382476	6	13	theme	zeta	682:685	arg1	potential					687:695	the zeta potential	678:695	the zeta potential	678:695	In addition, the hydrophobicity of sludge flocs was reduced and the zeta potential was more negative with the content of d-tyrosine increased.
24382476	6	13	theme	zeta	682:685	arg1	negative					706:713	negative	706:713	negative	706:713	In addition, the hydrophobicity of sludge flocs was reduced and the zeta potential was more negative with the content of d-tyrosine increased.
24382476	2	14	dep	stability	242:250	arg1	the					232:234	the	232:234	the	232:234	In this study, the effect of an exogenous d-tyrosine on the shear stability and surface characteristics of activated sludge flocs was investigated.
24382476	7	15	theme	polymeric	805:813	arg1	substances					815:824	extracellular polymeric substances	791:824	extracellular polymeric substances	791:824	A linear relationship between the extracellular polymeric substances and surface characteristics for sludge flocs indicated that the inhibited EPS production may be responsible for the instability of sludge upon the addition of d-tyrosine.
24382476	5	16	theme	EPS	577:579	arg1	fraction					581:588	EPS fraction	577:588	EPS fraction	577:588	A high correlation coefficient was observed between the composition of EPS fraction and d-tyrosine content.
24382476	2	17	theme	flocs	300:304	arg1	characteristics					264:278	surface characteristics	256:278	surface characteristics	256:278	In this study, the effect of an exogenous d-tyrosine on the shear stability and surface characteristics of activated sludge flocs was investigated.
24382476	2	17	theme	flocs	300:304	arg1	stability					242:250	shear stability	236:250	shear stability	236:250	In this study, the effect of an exogenous d-tyrosine on the shear stability and surface characteristics of activated sludge flocs was investigated.
24382476	5	18	theme	fraction	581:588	arg1	composition					562:572	the composition	558:572	the composition of EPS fraction and d-tyrosine content	558:611	A high correlation coefficient was observed between the composition of EPS fraction and d-tyrosine content.
24382476	2	19	theme	sludge	293:298	arg1	flocs					300:304	activated sludge flocs	283:304	activated sludge flocs	283:304	In this study, the effect of an exogenous d-tyrosine on the shear stability and surface characteristics of activated sludge flocs was investigated.
24382476	1	20	theme	floc	80:83	arg1	essential					98:106	essential	98:106	essential	98:106	The sludge floc stability is essential for the solid/liquid separation in biological wastewater treatment.
24382476	1	20	theme	floc	80:83	arg1	stability					85:93	The sludge floc stability	69:93	The sludge floc stability	69:93	The sludge floc stability is essential for the solid/liquid separation in biological wastewater treatment.
24382476	7	21	theme	d-tyrosine	985:994	arg1	addition					973:980	the addition	969:980	the addition of d-tyrosine	969:994	A linear relationship between the extracellular polymeric substances and surface characteristics for sludge flocs indicated that the inhibited EPS production may be responsible for the instability of sludge upon the addition of d-tyrosine.
24382476	4	22	theme	extracellular	436:448	arg1	EPS					472:474	EPS	472:474	EPS	472:474	d-Tyrosine inhibited the production of extracellular polymeric substances (EPS) especially for the proteins.
24382476	4	22	theme	extracellular	436:448	arg1	substances					460:469	extracellular polymeric substances	436:469	extracellular polymeric substances (EPS)	436:475	d-Tyrosine inhibited the production of extracellular polymeric substances (EPS) especially for the proteins.
24382476	2	23	theme	activated	283:291	arg1	flocs					300:304	activated sludge flocs	283:304	activated sludge flocs	283:304	In this study, the effect of an exogenous d-tyrosine on the shear stability and surface characteristics of activated sludge flocs was investigated.
24382476	3	24	theme	d-tyrosine	385:394	arg1	addition					373:380	the addition	369:380	the addition of d-tyrosine	369:394	Sludge flocs were found to be less stable in the addition of d-tyrosine.
24382476	7	25	theme	extracellular	791:803	arg1	substances					815:824	extracellular polymeric substances	791:824	extracellular polymeric substances	791:824	A linear relationship between the extracellular polymeric substances and surface characteristics for sludge flocs indicated that the inhibited EPS production may be responsible for the instability of sludge upon the addition of d-tyrosine.
24382476	2	26	theme	d-tyrosine	218:227	arg1	effect					195:200	the effect	191:200	the effect of an exogenous d-tyrosine on the shear stability and surface characteristics of activated sludge flocs	191:304	In this study, the effect of an exogenous d-tyrosine on the shear stability and surface characteristics of activated sludge flocs was investigated.
24382476	7	27	theme	surface	830:836	arg1	characteristics					838:852	surface characteristics	830:852	surface characteristics	830:852	A linear relationship between the extracellular polymeric substances and surface characteristics for sludge flocs indicated that the inhibited EPS production may be responsible for the instability of sludge upon the addition of d-tyrosine.
24382476	4	28	theme	substances	460:469	arg1	production					422:431	the production	418:431	the production of extracellular polymeric substances (EPS) especially for the proteins	418:503	d-Tyrosine inhibited the production of extracellular polymeric substances (EPS) especially for the proteins.
24382476	2	29	theme	exogenous	208:216	arg1	d-tyrosine					218:227	an exogenous d-tyrosine	205:227	an exogenous d-tyrosine	205:227	In this study, the effect of an exogenous d-tyrosine on the shear stability and surface characteristics of activated sludge flocs was investigated.
24382476	0	30	theme	activated	45:53	arg1	flocs					62:66	activated sludge flocs	45:66	activated sludge flocs	45:66	Influences of D-tyrosine on the stability of activated sludge flocs.
24382476	4	31	theme	polymeric	450:458	arg1	EPS					472:474	EPS	472:474	EPS	472:474	d-Tyrosine inhibited the production of extracellular polymeric substances (EPS) especially for the proteins.
24382476	4	31	theme	polymeric	450:458	arg1	substances					460:469	extracellular polymeric substances	436:469	extracellular polymeric substances (EPS)	436:475	d-Tyrosine inhibited the production of extracellular polymeric substances (EPS) especially for the proteins.
24382476	7	32	dep	substances	815:824	arg1	the					787:789	the	787:789	the	787:789	A linear relationship between the extracellular polymeric substances and surface characteristics for sludge flocs indicated that the inhibited EPS production may be responsible for the instability of sludge upon the addition of d-tyrosine.
24382476	7	33	theme	sludge	957:962	arg1	instability					942:952	the instability	938:952	the instability of sludge upon the addition of d-tyrosine	938:994	A linear relationship between the extracellular polymeric substances and surface characteristics for sludge flocs indicated that the inhibited EPS production may be responsible for the instability of sludge upon the addition of d-tyrosine.
24382476	6	34	theme	d-tyrosine	735:744	arg1	content					724:730	the content	720:730	the content of d-tyrosine	720:744	In addition, the hydrophobicity of sludge flocs was reduced and the zeta potential was more negative with the content of d-tyrosine increased.
24382476	6	35	theme	flocs	656:660	arg1	hydrophobicity					631:644	the hydrophobicity	627:644	the hydrophobicity of sludge flocs	627:660	In addition, the hydrophobicity of sludge flocs was reduced and the zeta potential was more negative with the content of d-tyrosine increased.
24382476	7	36	theme	linear	759:764	arg1	relationship					766:777	A linear relationship	757:777	A linear relationship between the extracellular polymeric substances and surface characteristics for sludge flocs	757:869	A linear relationship between the extracellular polymeric substances and surface characteristics for sludge flocs indicated that the inhibited EPS production may be responsible for the instability of sludge upon the addition of d-tyrosine.
24382476	6	37	theme	sludge	649:654	arg1	flocs					656:660	sludge flocs	649:660	sludge flocs	649:660	In addition, the hydrophobicity of sludge flocs was reduced and the zeta potential was more negative with the content of d-tyrosine increased.
24382476	2	38	theme	shear	236:240	arg1	stability					242:250	shear stability	236:250	shear stability	236:250	In this study, the effect of an exogenous d-tyrosine on the shear stability and surface characteristics of activated sludge flocs was investigated.
24382476	1	39	theme	solid/liquid	116:127	arg1	separation					129:138	the solid/liquid separation	112:138	the solid/liquid separation in biological wastewater treatment	112:173	The sludge floc stability is essential for the solid/liquid separation in biological wastewater treatment.
24382476	3	40	theme	Sludge	324:329	arg1	flocs					331:335	Sludge flocs	324:335	Sludge flocs	324:335	Sludge flocs were found to be less stable in the addition of d-tyrosine.
24382476	5	41	theme	high	508:511	arg1	coefficient					525:535	A high correlation coefficient	506:535	A high correlation coefficient	506:535	A high correlation coefficient was observed between the composition of EPS fraction and d-tyrosine content.
24382476	0	42	theme	sludge	55:60	arg1	flocs					62:66	activated sludge flocs	45:66	activated sludge flocs	45:66	Influences of D-tyrosine on the stability of activated sludge flocs.
24382476	3	43	from	addition	373:380	arg1	stable					359:364	stable	359:364	stable	359:364	Sludge flocs were found to be less stable in the addition of d-tyrosine.
24382476	0	44	theme	D-tyrosine	14:23	arg1	Influences					0:9	Influences	0:9	Influences of D-tyrosine on the stability of activated sludge flocs	0:66	Influences of D-tyrosine on the stability of activated sludge flocs.
24382476	5	45	theme	correlation	513:523	arg1	coefficient					525:535	A high correlation coefficient	506:535	A high correlation coefficient	506:535	A high correlation coefficient was observed between the composition of EPS fraction and d-tyrosine content.
24382476	7	46	theme	EPS	900:902	arg1	production					904:913	the inhibited EPS production	886:913	the inhibited EPS production	886:913	A linear relationship between the extracellular polymeric substances and surface characteristics for sludge flocs indicated that the inhibited EPS production may be responsible for the instability of sludge upon the addition of d-tyrosine.
24382476	7	46	theme	EPS	900:902	arg1	responsible					922:932	responsible	922:932	responsible	922:932	A linear relationship between the extracellular polymeric substances and surface characteristics for sludge flocs indicated that the inhibited EPS production may be responsible for the instability of sludge upon the addition of d-tyrosine.
28946314	5	0	theme	eating	922:927	arg1	quality					929:935	eating quality	922:935	eating quality	922:935	This study provides a structural basis to further investigate the starch degradation in kiwifruit, which may be of importance for the storage and eating quality of the fruit.
28946314	4	1	from	composition	659:669	arg1	pericarp					712:719	outer pericarp	706:719	outer pericarp	706:719	Overall, the composition and structure of starches from the outer pericarp and core tissues of a golden kiwifruit were different.
28946314	4	1	from	composition	659:669	arg1	tissues					730:736	core tissues	725:736	core tissues	725:736	Overall, the composition and structure of starches from the outer pericarp and core tissues of a golden kiwifruit were different.
28946314	1	2	theme	enzymatic	232:240	arg1	techniques					262:271	enzymatic and chromatographic techniques	232:271	enzymatic and chromatographic techniques	232:271	In this report, the amylose composition and molecular structure of starches from the core and outer pericarp of 3 golden kiwifruit varieties were characterised, using enzymatic and chromatographic techniques.
28946314	2	3	contain	have	326:329	arg2	contents					346:353	higher amylose contents	331:353	higher amylose contents (by ∼3-5%)	331:364	Starches from the core tissues of kiwifruit tend to have higher amylose contents (by ∼3-5%) and longer unit chains of both amylopectins and their φ, β-limit dextrins (LDs) than those of the outer pericarp starches.
28946314	2	3	contain	have	326:329	arg2	chains					382:387	longer unit chains	370:387	longer unit chains of both amylopectins and their φ, β-limit dextrins (LDs)	370:444	Starches from the core tissues of kiwifruit tend to have higher amylose contents (by ∼3-5%) and longer unit chains of both amylopectins and their φ, β-limit dextrins (LDs) than those of the outer pericarp starches.
28946314	2	3	contain	have	326:329	arg1	Starches					274:281	Starches	274:281	Starches from the core tissues of kiwifruit	274:316	Starches from the core tissues of kiwifruit tend to have higher amylose contents (by ∼3-5%) and longer unit chains of both amylopectins and their φ, β-limit dextrins (LDs) than those of the outer pericarp starches.
28946314	2	4	theme	amylose	338:344	arg1	contents					346:353	higher amylose contents	331:353	higher amylose contents (by ∼3-5%)	331:364	Starches from the core tissues of kiwifruit tend to have higher amylose contents (by ∼3-5%) and longer unit chains of both amylopectins and their φ, β-limit dextrins (LDs) than those of the outer pericarp starches.
28946314	2	5	theme	outer	464:468	arg1	starches					479:486	the outer pericarp starches	460:486	the outer pericarp starches	460:486	Starches from the core tissues of kiwifruit tend to have higher amylose contents (by ∼3-5%) and longer unit chains of both amylopectins and their φ, β-limit dextrins (LDs) than those of the outer pericarp starches.
28946314	4	6	theme	kiwifruit	750:758	arg1	pericarp					712:719	outer pericarp	706:719	outer pericarp	706:719	Overall, the composition and structure of starches from the outer pericarp and core tissues of a golden kiwifruit were different.
28946314	4	6	theme	kiwifruit	750:758	arg1	tissues					730:736	core tissues	725:736	core tissues	725:736	Overall, the composition and structure of starches from the outer pericarp and core tissues of a golden kiwifruit were different.
28946314	1	7	from	core	150:153	arg1	structure					119:127	molecular structure	109:127	molecular structure	109:127	In this report, the amylose composition and molecular structure of starches from the core and outer pericarp of 3 golden kiwifruit varieties were characterised, using enzymatic and chromatographic techniques.
28946314	1	7	from	core	150:153	arg1	starches					132:139	starches	132:139	starches from the core and outer pericarp of 3 golden kiwifruit varieties	132:204	In this report, the amylose composition and molecular structure of starches from the core and outer pericarp of 3 golden kiwifruit varieties were characterised, using enzymatic and chromatographic techniques.
28946314	1	7	from	core	150:153	arg1	composition					93:103	amylose composition	85:103	amylose composition	85:103	In this report, the amylose composition and molecular structure of starches from the core and outer pericarp of 3 golden kiwifruit varieties were characterised, using enzymatic and chromatographic techniques.
28946314	1	8	theme	chromatographic	246:260	arg1	techniques					262:271	enzymatic and chromatographic techniques	232:271	enzymatic and chromatographic techniques	232:271	In this report, the amylose composition and molecular structure of starches from the core and outer pericarp of 3 golden kiwifruit varieties were characterised, using enzymatic and chromatographic techniques.
28946314	3	9	theme	short	505:509	arg1	β-LDs					530:534	β-LDs	530:534	β-LDs of amylopectins from the outer pericarp	530:574	The contents of short B-chains of the φ, β-LDs of amylopectins from the outer pericarp were higher (by ∼3%) than those of φ, β-LDs of the core amylopectins.
28946314	3	9	theme	short	505:509	arg1	B-chains					511:518	short B-chains	505:518	short B-chains of the φ	505:527	The contents of short B-chains of the φ, β-LDs of amylopectins from the outer pericarp were higher (by ∼3%) than those of φ, β-LDs of the core amylopectins.
28946314	4	10	from	tissues	730:736	arg1	structure					675:683	structure	675:683	structure	675:683	Overall, the composition and structure of starches from the outer pericarp and core tissues of a golden kiwifruit were different.
28946314	4	10	from	tissues	730:736	arg1	composition					659:669	composition	659:669	composition	659:669	Overall, the composition and structure of starches from the outer pericarp and core tissues of a golden kiwifruit were different.
28946314	4	10	from	tissues	730:736	arg1	starches					688:695	starches	688:695	starches from the outer pericarp and core tissues of a golden kiwifruit	688:758	Overall, the composition and structure of starches from the outer pericarp and core tissues of a golden kiwifruit were different.
28946314	4	11	theme	golden	743:748	arg1	kiwifruit					750:758	a golden kiwifruit	741:758	a golden kiwifruit	741:758	Overall, the composition and structure of starches from the outer pericarp and core tissues of a golden kiwifruit were different.
28946314	5	12	theme	starch	842:847	arg1	degradation					849:859	the starch degradation	838:859	the starch degradation	838:859	This study provides a structural basis to further investigate the starch degradation in kiwifruit, which may be of importance for the storage and eating quality of the fruit.
28946314	3	13	from	β-LDs	530:534	arg1	pericarp					567:574	the outer pericarp	557:574	the outer pericarp	557:574	The contents of short B-chains of the φ, β-LDs of amylopectins from the outer pericarp were higher (by ∼3%) than those of φ, β-LDs of the core amylopectins.
28946314	5	14	dep	storage	910:916	arg1	the					906:908	the	906:908	the	906:908	This study provides a structural basis to further investigate the starch degradation in kiwifruit, which may be of importance for the storage and eating quality of the fruit.
28946314	3	15	from	pericarp	567:574	arg1	amylopectins					539:550	amylopectins	539:550	amylopectins from the outer pericarp	539:574	The contents of short B-chains of the φ, β-LDs of amylopectins from the outer pericarp were higher (by ∼3%) than those of φ, β-LDs of the core amylopectins.
28946314	3	15	from	pericarp	567:574	arg1	β-LDs					530:534	β-LDs	530:534	β-LDs of amylopectins from the outer pericarp	530:574	The contents of short B-chains of the φ, β-LDs of amylopectins from the outer pericarp were higher (by ∼3%) than those of φ, β-LDs of the core amylopectins.
28946314	3	15	from	pericarp	567:574	arg1	B-chains					511:518	short B-chains	505:518	short B-chains of the φ	505:527	The contents of short B-chains of the φ, β-LDs of amylopectins from the outer pericarp were higher (by ∼3%) than those of φ, β-LDs of the core amylopectins.
28946314	5	16	theme	fruit	944:948	arg1	storage					910:916	storage	910:916	storage	910:916	This study provides a structural basis to further investigate the starch degradation in kiwifruit, which may be of importance for the storage and eating quality of the fruit.
28946314	5	16	theme	fruit	944:948	arg1	quality					929:935	eating quality	922:935	eating quality	922:935	This study provides a structural basis to further investigate the starch degradation in kiwifruit, which may be of importance for the storage and eating quality of the fruit.
28946314	2	17	theme	φ	420:420	arg1	contents					346:353	higher amylose contents	331:353	higher amylose contents (by ∼3-5%)	331:364	Starches from the core tissues of kiwifruit tend to have higher amylose contents (by ∼3-5%) and longer unit chains of both amylopectins and their φ, β-limit dextrins (LDs) than those of the outer pericarp starches.
28946314	2	17	theme	φ	420:420	arg1	chains					382:387	longer unit chains	370:387	longer unit chains of both amylopectins and their φ, β-limit dextrins (LDs)	370:444	Starches from the core tissues of kiwifruit tend to have higher amylose contents (by ∼3-5%) and longer unit chains of both amylopectins and their φ, β-limit dextrins (LDs) than those of the outer pericarp starches.
28946314	3	18	dep	higher	581:586	arg1	%					594:594	∼3%	592:594	∼3%	592:594	The contents of short B-chains of the φ, β-LDs of amylopectins from the outer pericarp were higher (by ∼3%) than those of φ, β-LDs of the core amylopectins.
28946314	1	19	from	structure	119:127	arg1	pericarp					165:172	outer pericarp	159:172	outer pericarp	159:172	In this report, the amylose composition and molecular structure of starches from the core and outer pericarp of 3 golden kiwifruit varieties were characterised, using enzymatic and chromatographic techniques.
28946314	1	19	from	structure	119:127	arg1	core					150:153	the core	146:153	the core	146:153	In this report, the amylose composition and molecular structure of starches from the core and outer pericarp of 3 golden kiwifruit varieties were characterised, using enzymatic and chromatographic techniques.
28946314	1	20	theme	outer	159:163	arg1	pericarp					165:172	outer pericarp	159:172	outer pericarp	159:172	In this report, the amylose composition and molecular structure of starches from the core and outer pericarp of 3 golden kiwifruit varieties were characterised, using enzymatic and chromatographic techniques.
28946314	2	21	theme	β-limit	423:429	arg1	LDs					441:443	LDs	441:443	LDs	441:443	Starches from the core tissues of kiwifruit tend to have higher amylose contents (by ∼3-5%) and longer unit chains of both amylopectins and their φ, β-limit dextrins (LDs) than those of the outer pericarp starches.
28946314	2	21	theme	β-limit	423:429	arg1	φ					420:420	their φ	414:420	their φ	414:420	Starches from the core tissues of kiwifruit tend to have higher amylose contents (by ∼3-5%) and longer unit chains of both amylopectins and their φ, β-limit dextrins (LDs) than those of the outer pericarp starches.
28946314	2	21	theme	β-limit	423:429	arg1	dextrins					431:438	β-limit dextrins	423:438	β-limit dextrins (LDs)	423:444	Starches from the core tissues of kiwifruit tend to have higher amylose contents (by ∼3-5%) and longer unit chains of both amylopectins and their φ, β-limit dextrins (LDs) than those of the outer pericarp starches.
28946314	4	22	from	pericarp	712:719	arg1	structure					675:683	structure	675:683	structure	675:683	Overall, the composition and structure of starches from the outer pericarp and core tissues of a golden kiwifruit were different.
28946314	4	22	from	pericarp	712:719	arg1	composition					659:669	composition	659:669	composition	659:669	Overall, the composition and structure of starches from the outer pericarp and core tissues of a golden kiwifruit were different.
28946314	4	22	from	pericarp	712:719	arg1	starches					688:695	starches	688:695	starches from the outer pericarp and core tissues of a golden kiwifruit	688:758	Overall, the composition and structure of starches from the outer pericarp and core tissues of a golden kiwifruit were different.
28946314	2	23	theme	kiwifruit	308:316	arg1	tissues					297:303	the core tissues	288:303	the core tissues of kiwifruit	288:316	Starches from the core tissues of kiwifruit tend to have higher amylose contents (by ∼3-5%) and longer unit chains of both amylopectins and their φ, β-limit dextrins (LDs) than those of the outer pericarp starches.
28946314	3	24	theme	core	627:630	arg1	amylopectins					632:643	the core amylopectins	623:643	the core amylopectins	623:643	The contents of short B-chains of the φ, β-LDs of amylopectins from the outer pericarp were higher (by ∼3%) than those of φ, β-LDs of the core amylopectins.
28946314	4	25	theme	core	725:728	arg1	tissues					730:736	core tissues	725:736	core tissues	725:736	Overall, the composition and structure of starches from the outer pericarp and core tissues of a golden kiwifruit were different.
28946314	3	26	theme	B-chains	511:518	arg1	higher					581:586	higher	581:586	higher	581:586	The contents of short B-chains of the φ, β-LDs of amylopectins from the outer pericarp were higher (by ∼3%) than those of φ, β-LDs of the core amylopectins.
28946314	3	26	theme	B-chains	511:518	arg1	contents					493:500	The contents	489:500	The contents of short B-chains of the φ, β-LDs of amylopectins from the outer pericarp	489:574	The contents of short B-chains of the φ, β-LDs of amylopectins from the outer pericarp were higher (by ∼3%) than those of φ, β-LDs of the core amylopectins.
28946314	4	27	dep	composition	659:669	arg1	the					655:657	the	655:657	the	655:657	Overall, the composition and structure of starches from the outer pericarp and core tissues of a golden kiwifruit were different.
28946314	0	28	theme	chain	28:32	arg1	fractions					34:42	polymer chain fractions	20:42	polymer chain fractions of kiwifruit starch	20:62	Characterization of polymer chain fractions of kiwifruit starch.
28946314	3	29	theme	amylopectins	632:643	arg1	φ					611:611	φ	611:611	φ	611:611	The contents of short B-chains of the φ, β-LDs of amylopectins from the outer pericarp were higher (by ∼3%) than those of φ, β-LDs of the core amylopectins.
28946314	3	29	theme	amylopectins	632:643	arg1	β-LDs					614:618	β-LDs	614:618	β-LDs of the core amylopectins	614:643	The contents of short B-chains of the φ, β-LDs of amylopectins from the outer pericarp were higher (by ∼3%) than those of φ, β-LDs of the core amylopectins.
28946314	1	30	theme	amylose	85:91	arg1	composition					93:103	amylose composition	85:103	amylose composition	85:103	In this report, the amylose composition and molecular structure of starches from the core and outer pericarp of 3 golden kiwifruit varieties were characterised, using enzymatic and chromatographic techniques.
28946314	0	31	theme	polymer	20:26	arg1	fractions					34:42	polymer chain fractions	20:42	polymer chain fractions of kiwifruit starch	20:62	Characterization of polymer chain fractions of kiwifruit starch.
28946314	2	32	theme	core	292:295	arg1	tissues					297:303	the core tissues	288:303	the core tissues of kiwifruit	288:316	Starches from the core tissues of kiwifruit tend to have higher amylose contents (by ∼3-5%) and longer unit chains of both amylopectins and their φ, β-limit dextrins (LDs) than those of the outer pericarp starches.
28946314	1	33	theme	golden	179:184	arg1	varieties					196:204	3 golden kiwifruit varieties	177:204	3 golden kiwifruit varieties	177:204	In this report, the amylose composition and molecular structure of starches from the core and outer pericarp of 3 golden kiwifruit varieties were characterised, using enzymatic and chromatographic techniques.
28946314	1	34	theme	kiwifruit	186:194	arg1	varieties					196:204	3 golden kiwifruit varieties	177:204	3 golden kiwifruit varieties	177:204	In this report, the amylose composition and molecular structure of starches from the core and outer pericarp of 3 golden kiwifruit varieties were characterised, using enzymatic and chromatographic techniques.
28946314	3	35	theme	φ	527:527	arg1	β-LDs					530:534	β-LDs	530:534	β-LDs of amylopectins from the outer pericarp	530:574	The contents of short B-chains of the φ, β-LDs of amylopectins from the outer pericarp were higher (by ∼3%) than those of φ, β-LDs of the core amylopectins.
28946314	3	35	theme	φ	527:527	arg1	B-chains					511:518	short B-chains	505:518	short B-chains of the φ	505:527	The contents of short B-chains of the φ, β-LDs of amylopectins from the outer pericarp were higher (by ∼3%) than those of φ, β-LDs of the core amylopectins.
28946314	2	36	theme	unit	377:380	arg1	chains					382:387	longer unit chains	370:387	longer unit chains of both amylopectins and their φ, β-limit dextrins (LDs)	370:444	Starches from the core tissues of kiwifruit tend to have higher amylose contents (by ∼3-5%) and longer unit chains of both amylopectins and their φ, β-limit dextrins (LDs) than those of the outer pericarp starches.
28946314	0	37	theme	fractions	34:42	arg1	Characterization					0:15	Characterization	0:15	Characterization of polymer chain fractions of kiwifruit starch.	0:63	Characterization of polymer chain fractions of kiwifruit starch.
28946314	2	38	theme	pericarp	470:477	arg1	starches					479:486	the outer pericarp starches	460:486	the outer pericarp starches	460:486	Starches from the core tissues of kiwifruit tend to have higher amylose contents (by ∼3-5%) and longer unit chains of both amylopectins and their φ, β-limit dextrins (LDs) than those of the outer pericarp starches.
28946314	2	39	theme	amylopectins	397:408	arg1	contents					346:353	higher amylose contents	331:353	higher amylose contents (by ∼3-5%)	331:364	Starches from the core tissues of kiwifruit tend to have higher amylose contents (by ∼3-5%) and longer unit chains of both amylopectins and their φ, β-limit dextrins (LDs) than those of the outer pericarp starches.
28946314	2	39	theme	amylopectins	397:408	arg1	chains					382:387	longer unit chains	370:387	longer unit chains of both amylopectins and their φ, β-limit dextrins (LDs)	370:444	Starches from the core tissues of kiwifruit tend to have higher amylose contents (by ∼3-5%) and longer unit chains of both amylopectins and their φ, β-limit dextrins (LDs) than those of the outer pericarp starches.
28946314	1	40	theme	varieties	196:204	arg1	pericarp					165:172	outer pericarp	159:172	outer pericarp	159:172	In this report, the amylose composition and molecular structure of starches from the core and outer pericarp of 3 golden kiwifruit varieties were characterised, using enzymatic and chromatographic techniques.
28946314	1	40	theme	varieties	196:204	arg1	core					150:153	the core	146:153	the core	146:153	In this report, the amylose composition and molecular structure of starches from the core and outer pericarp of 3 golden kiwifruit varieties were characterised, using enzymatic and chromatographic techniques.
28946314	0	41	theme	starch	57:62	arg1	fractions					34:42	polymer chain fractions	20:42	polymer chain fractions of kiwifruit starch	20:62	Characterization of polymer chain fractions of kiwifruit starch.
28946314	3	42	theme	outer	561:565	arg1	pericarp					567:574	the outer pericarp	557:574	the outer pericarp	557:574	The contents of short B-chains of the φ, β-LDs of amylopectins from the outer pericarp were higher (by ∼3%) than those of φ, β-LDs of the core amylopectins.
28946314	4	43	theme	outer	706:710	arg1	pericarp					712:719	outer pericarp	706:719	outer pericarp	706:719	Overall, the composition and structure of starches from the outer pericarp and core tissues of a golden kiwifruit were different.
28946314	2	44	theme	higher	331:336	arg1	contents					346:353	higher amylose contents	331:353	higher amylose contents (by ∼3-5%)	331:364	Starches from the core tissues of kiwifruit tend to have higher amylose contents (by ∼3-5%) and longer unit chains of both amylopectins and their φ, β-limit dextrins (LDs) than those of the outer pericarp starches.
28946314	0	45	theme	kiwifruit	47:55	arg1	starch					57:62	kiwifruit starch	47:62	kiwifruit starch	47:62	Characterization of polymer chain fractions of kiwifruit starch.
28946314	1	46	theme	molecular	109:117	arg1	structure					119:127	molecular structure	109:127	molecular structure	109:127	In this report, the amylose composition and molecular structure of starches from the core and outer pericarp of 3 golden kiwifruit varieties were characterised, using enzymatic and chromatographic techniques.
28946314	3	47	theme	amylopectins	539:550	arg1	β-LDs					530:534	β-LDs	530:534	β-LDs of amylopectins from the outer pericarp	530:574	The contents of short B-chains of the φ, β-LDs of amylopectins from the outer pericarp were higher (by ∼3%) than those of φ, β-LDs of the core amylopectins.
28946314	3	47	theme	amylopectins	539:550	arg1	B-chains					511:518	short B-chains	505:518	short B-chains of the φ	505:527	The contents of short B-chains of the φ, β-LDs of amylopectins from the outer pericarp were higher (by ∼3%) than those of φ, β-LDs of the core amylopectins.
28946314	4	48	theme	starches	688:695	arg1	structure					675:683	structure	675:683	structure	675:683	Overall, the composition and structure of starches from the outer pericarp and core tissues of a golden kiwifruit were different.
28946314	4	48	theme	starches	688:695	arg1	composition					659:669	composition	659:669	composition	659:669	Overall, the composition and structure of starches from the outer pericarp and core tissues of a golden kiwifruit were different.
28946314	1	49	from	composition	93:103	arg1	pericarp					165:172	outer pericarp	159:172	outer pericarp	159:172	In this report, the amylose composition and molecular structure of starches from the core and outer pericarp of 3 golden kiwifruit varieties were characterised, using enzymatic and chromatographic techniques.
28946314	1	49	from	composition	93:103	arg1	core					150:153	the core	146:153	the core	146:153	In this report, the amylose composition and molecular structure of starches from the core and outer pericarp of 3 golden kiwifruit varieties were characterised, using enzymatic and chromatographic techniques.
28946314	1	50	from	pericarp	165:172	arg1	structure					119:127	molecular structure	109:127	molecular structure	109:127	In this report, the amylose composition and molecular structure of starches from the core and outer pericarp of 3 golden kiwifruit varieties were characterised, using enzymatic and chromatographic techniques.
28946314	1	50	from	pericarp	165:172	arg1	starches					132:139	starches	132:139	starches from the core and outer pericarp of 3 golden kiwifruit varieties	132:204	In this report, the amylose composition and molecular structure of starches from the core and outer pericarp of 3 golden kiwifruit varieties were characterised, using enzymatic and chromatographic techniques.
28946314	1	50	from	pericarp	165:172	arg1	composition					93:103	amylose composition	85:103	amylose composition	85:103	In this report, the amylose composition and molecular structure of starches from the core and outer pericarp of 3 golden kiwifruit varieties were characterised, using enzymatic and chromatographic techniques.
28946314	5	51	theme	structural	798:807	arg1	basis					809:813	a structural basis	796:813	a structural basis to further investigate the starch degradation in kiwifruit, which may be of importance for the storage and eating quality of the fruit	796:948	This study provides a structural basis to further investigate the starch degradation in kiwifruit, which may be of importance for the storage and eating quality of the fruit.
28946314	2	52	theme	longer	370:375	arg1	chains					382:387	longer unit chains	370:387	longer unit chains of both amylopectins and their φ, β-limit dextrins (LDs)	370:444	Starches from the core tissues of kiwifruit tend to have higher amylose contents (by ∼3-5%) and longer unit chains of both amylopectins and their φ, β-limit dextrins (LDs) than those of the outer pericarp starches.
28946314	4	53	from	structure	675:683	arg1	pericarp					712:719	outer pericarp	706:719	outer pericarp	706:719	Overall, the composition and structure of starches from the outer pericarp and core tissues of a golden kiwifruit were different.
28946314	4	53	from	structure	675:683	arg1	tissues					730:736	core tissues	725:736	core tissues	725:736	Overall, the composition and structure of starches from the outer pericarp and core tissues of a golden kiwifruit were different.
28946314	1	54	theme	starches	132:139	arg1	structure					119:127	molecular structure	109:127	molecular structure	109:127	In this report, the amylose composition and molecular structure of starches from the core and outer pericarp of 3 golden kiwifruit varieties were characterised, using enzymatic and chromatographic techniques.
28946314	1	54	theme	starches	132:139	arg1	composition					93:103	amylose composition	85:103	amylose composition	85:103	In this report, the amylose composition and molecular structure of starches from the core and outer pericarp of 3 golden kiwifruit varieties were characterised, using enzymatic and chromatographic techniques.
28946314	5	55	from	degradation	849:859	arg1	kiwifruit					864:872	kiwifruit	864:872	kiwifruit	864:872	This study provides a structural basis to further investigate the starch degradation in kiwifruit, which may be of importance for the storage and eating quality of the fruit.
28946314	4	56	dep	pericarp	712:719	arg1	the					702:704	the	702:704	the	702:704	Overall, the composition and structure of starches from the outer pericarp and core tissues of a golden kiwifruit were different.
28946314	1	57	dep	composition	93:103	arg1	the					81:83	the	81:83	the	81:83	In this report, the amylose composition and molecular structure of starches from the core and outer pericarp of 3 golden kiwifruit varieties were characterised, using enzymatic and chromatographic techniques.
28946314	2	58	from	tissues	297:303	arg1	Starches					274:281	Starches	274:281	Starches from the core tissues of kiwifruit	274:316	Starches from the core tissues of kiwifruit tend to have higher amylose contents (by ∼3-5%) and longer unit chains of both amylopectins and their φ, β-limit dextrins (LDs) than those of the outer pericarp starches.
25799321	8	0	theme	strong	1404:1409	arg1	linkage					1424:1430	a covalent or strong non-covalent linkage	1390:1430	linkage	1424:1430	The data, combined with a quantitative model for the evolution of the distribution during degradation, suggest that the bonding between β into α particles is different between phytoglycogen and liver glycogen, with the formation of a glycosidic linkage for phytoglycogen and a covalent or strong non-covalent linkage, most probably involving a protein, for glycogen as most likely.
25799321	4	1	theme	particles	657:665	arg1	formation					636:644	the formation	632:644	the formation of large α particles	632:665	This study aims to enhance our understanding of the nature of the link between liver-glycogen β particles resulting in the formation of large α particles.
25799321	7	2	theme	density	1036:1042	arg1	dependence					1008:1017	The size dependence	999:1017	The size dependence of the molecular density	999:1042	The size dependence of the molecular density shows qualitatively different shapes for these two types of molecules.
25799321	6	3	theme	phytoglycogen	863:875	arg1	distribution					847:858	The monomodal distribution	833:858	The monomodal distribution of phytoglycogen	833:875	The monomodal distribution of phytoglycogen decreases uniformly in time with hydrolysis, while with glycogen, the large particles degrade significantly more quickly.
25799321	2	4	theme	α	337:337	arg1	particles					339:347	composite α particles	327:347	composite α particles from smaller β particles	327:372	Both appear to form composite α particles from smaller β particles.
25799321	6	5	theme	large	947:951	arg1	particles					953:961	the large particles	943:961	the large particles	943:961	The monomodal distribution of phytoglycogen decreases uniformly in time with hydrolysis, while with glycogen, the large particles degrade significantly more quickly.
25799321	5	6	theme	time	684:687	arg1	evolution					689:697	the time evolution	680:697	the time evolution of the size distribution of these molecules during acid hydrolysis	680:764	It examines the time evolution of the size distribution of these molecules during acid hydrolysis, and the size dependence of the molecular density of both glucans.
25799321	0	7	theme	glycogen	106:113	arg1	particles					129:137	glycogen α (composite) particles	106:137	glycogen α (composite) particles	106:137	Acid hydrolysis and molecular density of phytoglycogen and liver glycogen helps understand the bonding in glycogen α (composite) particles.
25799321	6	8	theme	monomodal	837:845	arg1	distribution					847:858	The monomodal distribution	833:858	The monomodal distribution of phytoglycogen	833:875	The monomodal distribution of phytoglycogen decreases uniformly in time with hydrolysis, while with glycogen, the large particles degrade significantly more quickly.
25799321	7	9	theme	different	1064:1072	arg1	shapes					1074:1079	qualitatively different shapes	1050:1079	qualitatively different shapes	1050:1079	The size dependence of the molecular density shows qualitatively different shapes for these two types of molecules.
25799321	3	10	theme	liver	410:414	arg1	glycogen					416:423	liver glycogen	410:423	liver glycogen	410:423	The molecular size distribution of liver glycogen is bimodal, with distinct α and β components, while that of phytoglycogen is monomodal.
25799321	5	11	theme	density	808:814	arg1	dependence					780:789	the size dependence	771:789	the size dependence of the molecular density of both glucans	771:830	It examines the time evolution of the size distribution of these molecules during acid hydrolysis, and the size dependence of the molecular density of both glucans.
25799321	5	11	theme	density	808:814	arg1	evolution					689:697	the time evolution	680:697	the time evolution of the size distribution of these molecules during acid hydrolysis	680:764	It examines the time evolution of the size distribution of these molecules during acid hydrolysis, and the size dependence of the molecular density of both glucans.
25799321	3	12	theme	size	389:392	arg1	bimodal					428:434	bimodal	428:434	bimodal	428:434	The molecular size distribution of liver glycogen is bimodal, with distinct α and β components, while that of phytoglycogen is monomodal.
25799321	3	12	theme	size	389:392	arg1	distribution					394:405	The molecular size distribution	375:405	The molecular size distribution of liver glycogen	375:423	The molecular size distribution of liver glycogen is bimodal, with distinct α and β components, while that of phytoglycogen is monomodal.
25799321	1	13	theme	certain	160:166	arg1	plants					175:180	certain mutant plants	160:180	certain mutant plants	160:180	Phytoglycogen (from certain mutant plants) and animal glycogen are highly branched glucose polymers with similarities in structural features and molecular size range.
25799321	8	14	theme	quantitative	1141:1152	arg1	model					1154:1158	a quantitative model	1139:1158	a quantitative model for the evolution of the distribution during degradation	1139:1215	The data, combined with a quantitative model for the evolution of the distribution during degradation, suggest that the bonding between β into α particles is different between phytoglycogen and liver glycogen, with the formation of a glycosidic linkage for phytoglycogen and a covalent or strong non-covalent linkage, most probably involving a protein, for glycogen as most likely.
25799321	5	15	theme	size	706:709	arg1	distribution					711:722	the size distribution	702:722	the size distribution of these molecules during acid hydrolysis	702:764	It examines the time evolution of the size distribution of these molecules during acid hydrolysis, and the size dependence of the molecular density of both glucans.
25799321	6	16	with	time	900:903	arg1	hydrolysis					910:919	hydrolysis	910:919	hydrolysis	910:919	The monomodal distribution of phytoglycogen decreases uniformly in time with hydrolysis, while with glycogen, the large particles degrade significantly more quickly.
25799321	1	17	theme	mutant	168:173	arg1	plants					175:180	certain mutant plants	160:180	certain mutant plants	160:180	Phytoglycogen (from certain mutant plants) and animal glycogen are highly branched glucose polymers with similarities in structural features and molecular size range.
25799321	0	18	theme	Acid	0:3	arg1	hydrolysis					5:14	Acid hydrolysis	0:14	Acid hydrolysis	0:14	Acid hydrolysis and molecular density of phytoglycogen and liver glycogen helps understand the bonding in glycogen α (composite) particles.
25799321	1	19	theme	structural	261:270	arg1	features					272:279	structural features	261:279	structural features	261:279	Phytoglycogen (from certain mutant plants) and animal glycogen are highly branched glucose polymers with similarities in structural features and molecular size range.
25799321	2	20	theme	β	362:362	arg1	particles					364:372	smaller β particles	354:372	smaller β particles	354:372	Both appear to form composite α particles from smaller β particles.
25799321	0	21	theme	molecular	20:28	arg1	density					30:36	molecular density	20:36	molecular density	20:36	Acid hydrolysis and molecular density of phytoglycogen and liver glycogen helps understand the bonding in glycogen α (composite) particles.
25799321	1	22	from	similarities	245:256	arg1	range					300:304	molecular size range	285:304	molecular size range	285:304	Phytoglycogen (from certain mutant plants) and animal glycogen are highly branched glucose polymers with similarities in structural features and molecular size range.
25799321	1	22	from	similarities	245:256	arg1	features					272:279	structural features	261:279	structural features	261:279	Phytoglycogen (from certain mutant plants) and animal glycogen are highly branched glucose polymers with similarities in structural features and molecular size range.
25799321	9	23	theme	α-particle	1547:1556	arg1	structure					1558:1566	α-particle structure	1547:1566	α-particle structure	1547:1566	This finding is of importance for diabetes, where α-particle structure is impaired.
25799321	5	24	theme	acid	750:753	arg1	hydrolysis					755:764	acid hydrolysis	750:764	acid hydrolysis	750:764	It examines the time evolution of the size distribution of these molecules during acid hydrolysis, and the size dependence of the molecular density of both glucans.
25799321	3	25	theme	distinct	442:449	arg1	α					451:451	distinct α	442:451	distinct α	442:451	The molecular size distribution of liver glycogen is bimodal, with distinct α and β components, while that of phytoglycogen is monomodal.
25799321	5	26	theme	size	775:778	arg1	dependence					780:789	the size dependence	771:789	the size dependence of the molecular density of both glucans	771:830	It examines the time evolution of the size distribution of these molecules during acid hydrolysis, and the size dependence of the molecular density of both glucans.
25799321	0	27	theme	α	115:115	arg1	particles					129:137	glycogen α (composite) particles	106:137	glycogen α (composite) particles	106:137	Acid hydrolysis and molecular density of phytoglycogen and liver glycogen helps understand the bonding in glycogen α (composite) particles.
25799321	4	28	theme	α	655:655	arg1	particles					657:665	large α particles	649:665	large α particles	649:665	This study aims to enhance our understanding of the nature of the link between liver-glycogen β particles resulting in the formation of large α particles.
25799321	4	29	theme	large	649:653	arg1	particles					657:665	large α particles	649:665	large α particles	649:665	This study aims to enhance our understanding of the nature of the link between liver-glycogen β particles resulting in the formation of large α particles.
25799321	5	30	theme	molecules	733:741	arg1	distribution					711:722	the size distribution	702:722	the size distribution of these molecules during acid hydrolysis	702:764	It examines the time evolution of the size distribution of these molecules during acid hydrolysis, and the size dependence of the molecular density of both glucans.
25799321	1	31	theme	molecular	285:293	arg1	range					300:304	molecular size range	285:304	molecular size range	285:304	Phytoglycogen (from certain mutant plants) and animal glycogen are highly branched glucose polymers with similarities in structural features and molecular size range.
25799321	3	32	theme	glycogen	416:423	arg1	bimodal					428:434	bimodal	428:434	bimodal	428:434	The molecular size distribution of liver glycogen is bimodal, with distinct α and β components, while that of phytoglycogen is monomodal.
25799321	3	32	theme	glycogen	416:423	arg1	distribution					394:405	The molecular size distribution	375:405	The molecular size distribution of liver glycogen	375:423	The molecular size distribution of liver glycogen is bimodal, with distinct α and β components, while that of phytoglycogen is monomodal.
25799321	1	33	theme	animal	187:192	arg1	Phytoglycogen					140:152	Phytoglycogen	140:152	Phytoglycogen (from certain mutant plants)	140:181	Phytoglycogen (from certain mutant plants) and animal glycogen are highly branched glucose polymers with similarities in structural features and molecular size range.
25799321	1	33	theme	animal	187:192	arg1	polymers					231:238	highly branched glucose polymers	207:238	highly branched glucose polymers with similarities in structural features and molecular size range	207:304	Phytoglycogen (from certain mutant plants) and animal glycogen are highly branched glucose polymers with similarities in structural features and molecular size range.
25799321	1	33	theme	animal	187:192	arg1	glycogen					194:201	animal glycogen	187:201	animal glycogen	187:201	Phytoglycogen (from certain mutant plants) and animal glycogen are highly branched glucose polymers with similarities in structural features and molecular size range.
25799321	1	34	theme	size	295:298	arg1	range					300:304	molecular size range	285:304	molecular size range	285:304	Phytoglycogen (from certain mutant plants) and animal glycogen are highly branched glucose polymers with similarities in structural features and molecular size range.
25799321	0	35	theme	composite	118:126	arg1	particles					129:137	glycogen α (composite) particles	106:137	glycogen α (composite) particles	106:137	Acid hydrolysis and molecular density of phytoglycogen and liver glycogen helps understand the bonding in glycogen α (composite) particles.
25799321	8	36	theme	non-covalent	1411:1422	arg1	linkage					1424:1430	a covalent or strong non-covalent linkage	1390:1430	linkage	1424:1430	The data, combined with a quantitative model for the evolution of the distribution during degradation, suggest that the bonding between β into α particles is different between phytoglycogen and liver glycogen, with the formation of a glycosidic linkage for phytoglycogen and a covalent or strong non-covalent linkage, most probably involving a protein, for glycogen as most likely.
25799321	4	37	theme	link	579:582	arg1	nature					565:570	the nature	561:570	the nature of the link between liver-glycogen β particles resulting in the formation of large α particles	561:665	This study aims to enhance our understanding of the nature of the link between liver-glycogen β particles resulting in the formation of large α particles.
25799321	2	38	from	particles	364:372	arg1	particles					339:347	composite α particles	327:347	composite α particles from smaller β particles	327:372	Both appear to form composite α particles from smaller β particles.
25799321	4	39	theme	β	607:607	arg1	particles					609:617	liver-glycogen β particles	592:617	liver-glycogen β particles resulting in the formation of large α particles	592:665	This study aims to enhance our understanding of the nature of the link between liver-glycogen β particles resulting in the formation of large α particles.
25799321	3	40	theme	molecular	379:387	arg1	bimodal					428:434	bimodal	428:434	bimodal	428:434	The molecular size distribution of liver glycogen is bimodal, with distinct α and β components, while that of phytoglycogen is monomodal.
25799321	3	40	theme	molecular	379:387	arg1	distribution					394:405	The molecular size distribution	375:405	The molecular size distribution of liver glycogen	375:423	The molecular size distribution of liver glycogen is bimodal, with distinct α and β components, while that of phytoglycogen is monomodal.
25799321	7	41	theme	size	1003:1006	arg1	dependence					1008:1017	The size dependence	999:1017	The size dependence of the molecular density	999:1042	The size dependence of the molecular density shows qualitatively different shapes for these two types of molecules.
25799321	5	42	theme	distribution	711:722	arg1	dependence					780:789	the size dependence	771:789	the size dependence of the molecular density of both glucans	771:830	It examines the time evolution of the size distribution of these molecules during acid hydrolysis, and the size dependence of the molecular density of both glucans.
25799321	5	42	theme	distribution	711:722	arg1	evolution					689:697	the time evolution	680:697	the time evolution of the size distribution of these molecules during acid hydrolysis	680:764	It examines the time evolution of the size distribution of these molecules during acid hydrolysis, and the size dependence of the molecular density of both glucans.
25799321	8	43	theme	linkage	1360:1366	arg1	formation					1334:1342	the formation	1330:1342	the formation of a glycosidic linkage for phytoglycogen and a covalent or strong non-covalent linkage	1330:1430	The data, combined with a quantitative model for the evolution of the distribution during degradation, suggest that the bonding between β into α particles is different between phytoglycogen and liver glycogen, with the formation of a glycosidic linkage for phytoglycogen and a covalent or strong non-covalent linkage, most probably involving a protein, for glycogen as most likely.
25799321	2	44	theme	composite	327:335	arg1	particles					339:347	composite α particles	327:347	composite α particles from smaller β particles	327:372	Both appear to form composite α particles from smaller β particles.
25799321	0	45	theme	phytoglycogen	41:53	arg1	hydrolysis					5:14	Acid hydrolysis	0:14	Acid hydrolysis	0:14	Acid hydrolysis and molecular density of phytoglycogen and liver glycogen helps understand the bonding in glycogen α (composite) particles.
25799321	0	45	theme	phytoglycogen	41:53	arg1	density					30:36	molecular density	20:36	molecular density	20:36	Acid hydrolysis and molecular density of phytoglycogen and liver glycogen helps understand the bonding in glycogen α (composite) particles.
25799321	7	46	theme	molecular	1026:1034	arg1	density					1036:1042	the molecular density	1022:1042	the molecular density	1022:1042	The size dependence of the molecular density shows qualitatively different shapes for these two types of molecules.
25799321	8	47	theme	glycosidic	1349:1358	arg1	linkage					1360:1366	a glycosidic linkage	1347:1366	a glycosidic linkage for phytoglycogen and a covalent or strong non-covalent linkage	1347:1430	The data, combined with a quantitative model for the evolution of the distribution during degradation, suggest that the bonding between β into α particles is different between phytoglycogen and liver glycogen, with the formation of a glycosidic linkage for phytoglycogen and a covalent or strong non-covalent linkage, most probably involving a protein, for glycogen as most likely.
25799321	1	48	with	polymers	231:238	arg1	similarities					245:256	similarities	245:256	similarities in structural features and molecular size range	245:304	Phytoglycogen (from certain mutant plants) and animal glycogen are highly branched glucose polymers with similarities in structural features and molecular size range.
25799321	8	49	with	different	1273:1281	arg1	formation					1334:1342	the formation	1330:1342	the formation of a glycosidic linkage for phytoglycogen and a covalent or strong non-covalent linkage	1330:1430	The data, combined with a quantitative model for the evolution of the distribution during degradation, suggest that the bonding between β into α particles is different between phytoglycogen and liver glycogen, with the formation of a glycosidic linkage for phytoglycogen and a covalent or strong non-covalent linkage, most probably involving a protein, for glycogen as most likely.
25799321	7	50	theme	molecules	1104:1112	arg1	types					1095:1099	these two types	1085:1099	these two types of molecules	1085:1112	The size dependence of the molecular density shows qualitatively different shapes for these two types of molecules.
25799321	8	51	theme	α	1258:1258	arg1	particles					1260:1268	α particles	1258:1268	α particles	1258:1268	The data, combined with a quantitative model for the evolution of the distribution during degradation, suggest that the bonding between β into α particles is different between phytoglycogen and liver glycogen, with the formation of a glycosidic linkage for phytoglycogen and a covalent or strong non-covalent linkage, most probably involving a protein, for glycogen as most likely.
25799321	5	52	theme	glucans	824:830	arg1	density					808:814	the molecular density	794:814	the molecular density of both glucans	794:830	It examines the time evolution of the size distribution of these molecules during acid hydrolysis, and the size dependence of the molecular density of both glucans.
25799321	3	53	with	bimodal	428:434	arg1	components					459:468	β components	457:468	β components	457:468	The molecular size distribution of liver glycogen is bimodal, with distinct α and β components, while that of phytoglycogen is monomodal.
25799321	3	53	with	bimodal	428:434	arg1	α					451:451	distinct α	442:451	distinct α	442:451	The molecular size distribution of liver glycogen is bimodal, with distinct α and β components, while that of phytoglycogen is monomodal.
25799321	0	54	from	bonding	95:101	arg1	particles					129:137	glycogen α (composite) particles	106:137	glycogen α (composite) particles	106:137	Acid hydrolysis and molecular density of phytoglycogen and liver glycogen helps understand the bonding in glycogen α (composite) particles.
25799321	3	55	theme	β	457:457	arg1	components					459:468	β components	457:468	β components	457:468	The molecular size distribution of liver glycogen is bimodal, with distinct α and β components, while that of phytoglycogen is monomodal.
25799321	4	56	theme	nature	565:570	arg1	understanding					544:556	our understanding	540:556	our understanding of the nature of the link between liver-glycogen β particles resulting in the formation of large α particles	540:665	This study aims to enhance our understanding of the nature of the link between liver-glycogen β particles resulting in the formation of large α particles.
25799321	0	57	theme	glycogen	65:72	arg1	hydrolysis					5:14	Acid hydrolysis	0:14	Acid hydrolysis	0:14	Acid hydrolysis and molecular density of phytoglycogen and liver glycogen helps understand the bonding in glycogen α (composite) particles.
25799321	0	57	theme	glycogen	65:72	arg1	density					30:36	molecular density	20:36	molecular density	20:36	Acid hydrolysis and molecular density of phytoglycogen and liver glycogen helps understand the bonding in glycogen α (composite) particles.
25799321	5	58	theme	molecular	798:806	arg1	density					808:814	the molecular density	794:814	the molecular density of both glucans	794:830	It examines the time evolution of the size distribution of these molecules during acid hydrolysis, and the size dependence of the molecular density of both glucans.
25799321	1	59	from	plants	175:180	arg1	Phytoglycogen					140:152	Phytoglycogen	140:152	Phytoglycogen (from certain mutant plants)	140:181	Phytoglycogen (from certain mutant plants) and animal glycogen are highly branched glucose polymers with similarities in structural features and molecular size range.
25799321	1	59	from	plants	175:180	arg1	polymers					231:238	highly branched glucose polymers	207:238	highly branched glucose polymers with similarities in structural features and molecular size range	207:304	Phytoglycogen (from certain mutant plants) and animal glycogen are highly branched glucose polymers with similarities in structural features and molecular size range.
25799321	1	59	from	plants	175:180	arg1	glycogen					194:201	animal glycogen	187:201	animal glycogen	187:201	Phytoglycogen (from certain mutant plants) and animal glycogen are highly branched glucose polymers with similarities in structural features and molecular size range.
25799321	2	60	theme	smaller	354:360	arg1	particles					364:372	smaller β particles	354:372	smaller β particles	354:372	Both appear to form composite α particles from smaller β particles.
25799321	8	61	theme	distribution	1185:1196	arg1	evolution					1168:1176	the evolution	1164:1176	the evolution of the distribution during degradation	1164:1215	The data, combined with a quantitative model for the evolution of the distribution during degradation, suggest that the bonding between β into α particles is different between phytoglycogen and liver glycogen, with the formation of a glycosidic linkage for phytoglycogen and a covalent or strong non-covalent linkage, most probably involving a protein, for glycogen as most likely.
25799321	1	62	theme	branched	214:221	arg1	Phytoglycogen					140:152	Phytoglycogen	140:152	Phytoglycogen (from certain mutant plants)	140:181	Phytoglycogen (from certain mutant plants) and animal glycogen are highly branched glucose polymers with similarities in structural features and molecular size range.
25799321	1	62	theme	branched	214:221	arg1	polymers					231:238	highly branched glucose polymers	207:238	highly branched glucose polymers with similarities in structural features and molecular size range	207:304	Phytoglycogen (from certain mutant plants) and animal glycogen are highly branched glucose polymers with similarities in structural features and molecular size range.
25799321	1	62	theme	branched	214:221	arg1	glycogen					194:201	animal glycogen	187:201	animal glycogen	187:201	Phytoglycogen (from certain mutant plants) and animal glycogen are highly branched glucose polymers with similarities in structural features and molecular size range.
25799321	0	63	theme	liver	59:63	arg1	glycogen					65:72	liver glycogen	59:72	liver glycogen	59:72	Acid hydrolysis and molecular density of phytoglycogen and liver glycogen helps understand the bonding in glycogen α (composite) particles.
25799321	4	64	theme	liver-glycogen	592:605	arg1	particles					609:617	liver-glycogen β particles	592:617	liver-glycogen β particles resulting in the formation of large α particles	592:665	This study aims to enhance our understanding of the nature of the link between liver-glycogen β particles resulting in the formation of large α particles.
25799321	1	65	theme	glucose	223:229	arg1	Phytoglycogen					140:152	Phytoglycogen	140:152	Phytoglycogen (from certain mutant plants)	140:181	Phytoglycogen (from certain mutant plants) and animal glycogen are highly branched glucose polymers with similarities in structural features and molecular size range.
25799321	1	65	theme	glucose	223:229	arg1	polymers					231:238	highly branched glucose polymers	207:238	highly branched glucose polymers with similarities in structural features and molecular size range	207:304	Phytoglycogen (from certain mutant plants) and animal glycogen are highly branched glucose polymers with similarities in structural features and molecular size range.
25799321	1	65	theme	glucose	223:229	arg1	glycogen					194:201	animal glycogen	187:201	animal glycogen	187:201	Phytoglycogen (from certain mutant plants) and animal glycogen are highly branched glucose polymers with similarities in structural features and molecular size range.
25240187	1	0	theme	Hellecaucaside	151:164	arg1	nucleoside					188:197	a new disaccharide nucleoside	169:197	a new disaccharide nucleoside featuring a 2'-O-α-D-ribofuranosyluridine skeleton and a 4-hydroxybenzoyl group at the 5' position	169:296	Hellecaucaside A, a new disaccharide nucleoside featuring a 2'-O-α-D-ribofuranosyluridine skeleton and a 4-hydroxybenzoyl group at the 5' position, was isolated from the underground part of Helleborus caucasicus.
25240187	1	0	theme	Hellecaucaside	151:164	arg1	A					166:166	Hellecaucaside A	151:166	Hellecaucaside A	151:166	Hellecaucaside A, a new disaccharide nucleoside featuring a 2'-O-α-D-ribofuranosyluridine skeleton and a 4-hydroxybenzoyl group at the 5' position, was isolated from the underground part of Helleborus caucasicus.
25240187	1	1	theme	4-hydroxybenzoyl	256:271	arg1	group					273:277	a 4-hydroxybenzoyl group	254:277	a 4-hydroxybenzoyl group at the 5' position	254:296	Hellecaucaside A, a new disaccharide nucleoside featuring a 2'-O-α-D-ribofuranosyluridine skeleton and a 4-hydroxybenzoyl group at the 5' position, was isolated from the underground part of Helleborus caucasicus.
25240187	2	2	theme	1D/2D	478:482	arg1	NMR					484:486	1D/2D NMR	478:486	1D/2D NMR	478:486	The structure of the compound was elucidated by means of chemical degradation and spectroscopic analyses, such as 1D/2D NMR, chiral-GC, and HRMS.
25240187	0	3	theme	total	97:101	arg1	synthesis					103:111	total synthesis	97:111	total synthesis	97:111	Isolation of a new disaccharide nucleoside from Helleborus caucasicus: structure elucidation and total synthesis of hellecaucaside A and its β-anomer.
25240187	1	4	theme	caucasicus	352:361	arg1	part					333:336	the underground part	317:336	the underground part of Helleborus caucasicus	317:361	Hellecaucaside A, a new disaccharide nucleoside featuring a 2'-O-α-D-ribofuranosyluridine skeleton and a 4-hydroxybenzoyl group at the 5' position, was isolated from the underground part of Helleborus caucasicus.
25240187	2	5	dep	such	470:473	arg1	as					475:476	as	475:476	as	475:476	The structure of the compound was elucidated by means of chemical degradation and spectroscopic analyses, such as 1D/2D NMR, chiral-GC, and HRMS.
25240187	3	6	theme	total	514:518	arg1	synthesis					520:528	The total synthesis	510:528	The total synthesis of hellecaucaside A and its β-anomer	510:565	The total synthesis of hellecaucaside A and its β-anomer was accomplished, unequivocally confirming the structure of the natural product.
25240187	2	7	theme	compound	385:392	arg1	structure					368:376	The structure	364:376	The structure of the compound	364:392	The structure of the compound was elucidated by means of chemical degradation and spectroscopic analyses, such as 1D/2D NMR, chiral-GC, and HRMS.
25240187	2	8	theme	spectroscopic	446:458	arg1	analyses					460:467	spectroscopic analyses	446:467	spectroscopic analyses	446:467	The structure of the compound was elucidated by means of chemical degradation and spectroscopic analyses, such as 1D/2D NMR, chiral-GC, and HRMS.
25240187	0	9	theme	structure	71:79	arg1	elucidation					81:91	structure elucidation	71:91	structure elucidation	71:91	Isolation of a new disaccharide nucleoside from Helleborus caucasicus: structure elucidation and total synthesis of hellecaucaside A and its β-anomer.
25240187	1	10	attach	isolated	303:310	arg2	A					166:166	Hellecaucaside A	151:166	Hellecaucaside A	151:166	Hellecaucaside A, a new disaccharide nucleoside featuring a 2'-O-α-D-ribofuranosyluridine skeleton and a 4-hydroxybenzoyl group at the 5' position, was isolated from the underground part of Helleborus caucasicus.
25240187	1	10	attach	isolated	303:310	arg1	part					333:336	the underground part	317:336	the underground part of Helleborus caucasicus	317:361	Hellecaucaside A, a new disaccharide nucleoside featuring a 2'-O-α-D-ribofuranosyluridine skeleton and a 4-hydroxybenzoyl group at the 5' position, was isolated from the underground part of Helleborus caucasicus.
25240187	1	10	attach	isolated	303:310	arg2	nucleoside					188:197	a new disaccharide nucleoside	169:197	a new disaccharide nucleoside featuring a 2'-O-α-D-ribofuranosyluridine skeleton and a 4-hydroxybenzoyl group at the 5' position	169:296	Hellecaucaside A, a new disaccharide nucleoside featuring a 2'-O-α-D-ribofuranosyluridine skeleton and a 4-hydroxybenzoyl group at the 5' position, was isolated from the underground part of Helleborus caucasicus.
25240187	1	11	theme	new	171:173	arg1	nucleoside					188:197	a new disaccharide nucleoside	169:197	a new disaccharide nucleoside featuring a 2'-O-α-D-ribofuranosyluridine skeleton and a 4-hydroxybenzoyl group at the 5' position	169:296	Hellecaucaside A, a new disaccharide nucleoside featuring a 2'-O-α-D-ribofuranosyluridine skeleton and a 4-hydroxybenzoyl group at the 5' position, was isolated from the underground part of Helleborus caucasicus.
25240187	1	11	theme	new	171:173	arg1	A					166:166	Hellecaucaside A	151:166	Hellecaucaside A	151:166	Hellecaucaside A, a new disaccharide nucleoside featuring a 2'-O-α-D-ribofuranosyluridine skeleton and a 4-hydroxybenzoyl group at the 5' position, was isolated from the underground part of Helleborus caucasicus.
25240187	1	12	theme	disaccharide	175:186	arg1	nucleoside					188:197	a new disaccharide nucleoside	169:197	a new disaccharide nucleoside featuring a 2'-O-α-D-ribofuranosyluridine skeleton and a 4-hydroxybenzoyl group at the 5' position	169:296	Hellecaucaside A, a new disaccharide nucleoside featuring a 2'-O-α-D-ribofuranosyluridine skeleton and a 4-hydroxybenzoyl group at the 5' position, was isolated from the underground part of Helleborus caucasicus.
25240187	1	12	theme	disaccharide	175:186	arg1	A					166:166	Hellecaucaside A	151:166	Hellecaucaside A	151:166	Hellecaucaside A, a new disaccharide nucleoside featuring a 2'-O-α-D-ribofuranosyluridine skeleton and a 4-hydroxybenzoyl group at the 5' position, was isolated from the underground part of Helleborus caucasicus.
25240187	0	13	theme	new	15:17	arg1	nucleoside					32:41	a new disaccharide nucleoside	13:41	a new disaccharide nucleoside	13:41	Isolation of a new disaccharide nucleoside from Helleborus caucasicus: structure elucidation and total synthesis of hellecaucaside A and its β-anomer.
25240187	0	14	theme	A	131:131	arg1	elucidation					81:91	structure elucidation	71:91	structure elucidation	71:91	Isolation of a new disaccharide nucleoside from Helleborus caucasicus: structure elucidation and total synthesis of hellecaucaside A and its β-anomer.
25240187	0	14	theme	A	131:131	arg1	synthesis					103:111	total synthesis	97:111	total synthesis	97:111	Isolation of a new disaccharide nucleoside from Helleborus caucasicus: structure elucidation and total synthesis of hellecaucaside A and its β-anomer.
25240187	0	14	theme	A	131:131	arg1	β-anomer					141:148	its β-anomer	137:148	its β-anomer	137:148	Isolation of a new disaccharide nucleoside from Helleborus caucasicus: structure elucidation and total synthesis of hellecaucaside A and its β-anomer.
25240187	3	15	theme	hellecaucaside	533:546	arg1	A					548:548	hellecaucaside A	533:548	hellecaucaside A	533:548	The total synthesis of hellecaucaside A and its β-anomer was accomplished, unequivocally confirming the structure of the natural product.
25240187	2	16	theme	chemical	421:428	arg1	degradation					430:440	chemical degradation	421:440	chemical degradation	421:440	The structure of the compound was elucidated by means of chemical degradation and spectroscopic analyses, such as 1D/2D NMR, chiral-GC, and HRMS.
25240187	0	17	theme	hellecaucaside	116:129	arg1	A					131:131	hellecaucaside A	116:131	hellecaucaside A	116:131	Isolation of a new disaccharide nucleoside from Helleborus caucasicus: structure elucidation and total synthesis of hellecaucaside A and its β-anomer.
25240187	3	18	theme	natural	631:637	arg1	product					639:645	the natural product	627:645	the natural product	627:645	The total synthesis of hellecaucaside A and its β-anomer was accomplished, unequivocally confirming the structure of the natural product.
25240187	3	19	theme	A	548:548	arg1	synthesis					520:528	The total synthesis	510:528	The total synthesis of hellecaucaside A and its β-anomer	510:565	The total synthesis of hellecaucaside A and its β-anomer was accomplished, unequivocally confirming the structure of the natural product.
25240187	0	20	theme	nucleoside	32:41	arg1	Isolation					0:8	Isolation	0:8	Isolation of a new disaccharide nucleoside from Helleborus caucasicus: structure elucidation and total synthesis of hellecaucaside A and its β-anomer.	0:149	Isolation of a new disaccharide nucleoside from Helleborus caucasicus: structure elucidation and total synthesis of hellecaucaside A and its β-anomer.
25240187	3	21	theme	product	639:645	arg1	structure					614:622	the structure	610:622	the structure of the natural product	610:645	The total synthesis of hellecaucaside A and its β-anomer was accomplished, unequivocally confirming the structure of the natural product.
25240187	0	22	theme	disaccharide	19:30	arg1	nucleoside					32:41	a new disaccharide nucleoside	13:41	a new disaccharide nucleoside	13:41	Isolation of a new disaccharide nucleoside from Helleborus caucasicus: structure elucidation and total synthesis of hellecaucaside A and its β-anomer.
25240187	1	23	theme	2'-O-	211:215	arg1	skeleton					241:248	a 2'-O-α-D-ribofuranosyluridine skeleton	209:248	a 2'-O-α-D-ribofuranosyluridine skeleton	209:248	Hellecaucaside A, a new disaccharide nucleoside featuring a 2'-O-α-D-ribofuranosyluridine skeleton and a 4-hydroxybenzoyl group at the 5' position, was isolated from the underground part of Helleborus caucasicus.
25240187	0	24	from	caucasicus	59:68	arg1	Isolation					0:8	Isolation	0:8	Isolation of a new disaccharide nucleoside from Helleborus caucasicus: structure elucidation and total synthesis of hellecaucaside A and its β-anomer.	0:149	Isolation of a new disaccharide nucleoside from Helleborus caucasicus: structure elucidation and total synthesis of hellecaucaside A and its β-anomer.
25240187	3	25	theme	β-anomer	558:565	arg1	synthesis					520:528	The total synthesis	510:528	The total synthesis of hellecaucaside A and its β-anomer	510:565	The total synthesis of hellecaucaside A and its β-anomer was accomplished, unequivocally confirming the structure of the natural product.
25240187	1	26	theme	α-D-ribofuranosyluridine	216:239	arg1	skeleton					241:248	a 2'-O-α-D-ribofuranosyluridine skeleton	209:248	a 2'-O-α-D-ribofuranosyluridine skeleton	209:248	Hellecaucaside A, a new disaccharide nucleoside featuring a 2'-O-α-D-ribofuranosyluridine skeleton and a 4-hydroxybenzoyl group at the 5' position, was isolated from the underground part of Helleborus caucasicus.
25240187	1	27	theme	underground	321:331	arg1	part					333:336	the underground part	317:336	the underground part of Helleborus caucasicus	317:361	Hellecaucaside A, a new disaccharide nucleoside featuring a 2'-O-α-D-ribofuranosyluridine skeleton and a 4-hydroxybenzoyl group at the 5' position, was isolated from the underground part of Helleborus caucasicus.
25240187	0	28	dep	Isolation	0:8	arg1	elucidation					81:91	structure elucidation	71:91	structure elucidation	71:91	Isolation of a new disaccharide nucleoside from Helleborus caucasicus: structure elucidation and total synthesis of hellecaucaside A and its β-anomer.
25240187	0	28	dep	Isolation	0:8	arg1	synthesis					103:111	total synthesis	97:111	total synthesis	97:111	Isolation of a new disaccharide nucleoside from Helleborus caucasicus: structure elucidation and total synthesis of hellecaucaside A and its β-anomer.
25240187	0	28	dep	Isolation	0:8	arg1	β-anomer					141:148	its β-anomer	137:148	its β-anomer	137:148	Isolation of a new disaccharide nucleoside from Helleborus caucasicus: structure elucidation and total synthesis of hellecaucaside A and its β-anomer.
26572362	5	0	theme	octahedral	1053:1062	arg1	catalyst					1064:1071	octahedral catalyst	1053:1071	octahedral catalyst	1053:1071	The CA supported cubic Ag@AgCl catalyst exhibited much higher catalytic activity than octahedral catalyst in terms of the degradation of MO under visible light.
26572362	1	1	theme	cellulose	182:190	arg1	CA					201:202	CA	201:202	CA	201:202	Electrospun cellulose acetate (CA) membrane was employed as a support that provided sites for AgCl crystals in situ growth.
26572362	1	1	theme	cellulose	182:190	arg1	acetate					192:198	Electrospun cellulose acetate	170:198	Electrospun cellulose acetate (CA) membrane	170:212	Electrospun cellulose acetate (CA) membrane was employed as a support that provided sites for AgCl crystals in situ growth.
26572362	3	2	theme	diffuse	827:833	arg1	spectra					847:853	energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra	757:853	spectra	847:853	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	3	2	theme	diffuse	827:833	arg1	intensity					684:692	total reflection-infrared intensity	658:692	total reflection-infrared intensity (ATR-IR)	658:701	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	3	3	theme	spectrometer	775:786	arg1	EDS					789:791	energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra	757:853	EDS	789:791	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	3	3	theme	spectrometer	775:786	arg1	intensity					684:692	total reflection-infrared intensity	658:692	total reflection-infrared intensity (ATR-IR)	658:701	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	0	4	theme	photocatalytic	80:93	arg1	properties					95:104	facet-dependent photocatalytic properties	64:104	facet-dependent photocatalytic properties	64:104	Electrospun cellulose acetate supported Ag@AgCl composites with facet-dependent photocatalytic properties on degradation of organic dyes under visible-light irradiation.
26572362	7	5	theme	good	1242:1245	arg1	ability					1257:1263	a good catalytic ability	1240:1263	a good catalytic ability	1240:1263	The photocatalyst still exhibited a good catalytic ability after three times.
26572362	5	6	theme	@	992:992	arg1	catalyst					998:1005	The CA supported cubic Ag@AgCl catalyst	967:1005	The CA supported cubic Ag@AgCl catalyst	967:1005	The CA supported cubic Ag@AgCl catalyst exhibited much higher catalytic activity than octahedral catalyst in terms of the degradation of MO under visible light.
26572362	1	7	theme	acetate	192:198	arg1	support					232:238	a support	230:238	a support that provided sites for AgCl crystals in situ growth	230:291	Electrospun cellulose acetate (CA) membrane was employed as a support that provided sites for AgCl crystals in situ growth.
26572362	1	7	theme	acetate	192:198	arg1	membrane					205:212	Electrospun cellulose acetate (CA) membrane	170:212	Electrospun cellulose acetate (CA) membrane	170:212	Electrospun cellulose acetate (CA) membrane was employed as a support that provided sites for AgCl crystals in situ growth.
26572362	2	8	from	crystals	306:313	arg1	composites					333:342	electrospun CA composites	318:342	electrospun CA composites with exposed {100} and {111} facets	318:378	The Ag@AgCl crystals on electrospun CA composites with exposed {100} and {111} facets were fabricated at room temperature by a double diffusion technique.
26572362	1	9	theme	AgCl	264:267	arg1	crystals					269:276	AgCl crystals	264:276	AgCl crystals in situ growth	264:291	Electrospun cellulose acetate (CA) membrane was employed as a support that provided sites for AgCl crystals in situ growth.
26572362	6	10	theme	mg	1184:1185	arg1	MO					1177:1178	MO	1177:1178	MO (10 mg L(-1))	1177:1192	The 10mg CA based cubes could completely degrade MO (10 mg L(-1)) in 160 min.
26572362	6	10	theme	mg	1184:1185	arg1	L					1187:1187	10 mg L(-1)	1181:1191	10 mg L(-1)	1181:1191	The 10mg CA based cubes could completely degrade MO (10 mg L(-1)) in 160 min.
26572362	6	11	theme	based	1140:1144	arg1	cubes					1146:1150	The 10mg CA based cubes	1128:1150	The 10mg CA based cubes	1128:1150	The 10mg CA based cubes could completely degrade MO (10 mg L(-1)) in 160 min.
26572362	3	12	theme	absorption	501:510	arg1	ability					518:524	absorption light ability	501:524	absorption light ability	501:524	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	3	13	theme	UV-vis	819:824	arg1	spectra					847:853	energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra	757:853	spectra	847:853	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	3	13	theme	UV-vis	819:824	arg1	intensity					684:692	total reflection-infrared intensity	658:692	total reflection-infrared intensity (ATR-IR)	658:701	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	3	14	theme	energy	757:762	arg1	EDS					789:791	energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra	757:853	EDS	789:791	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	3	14	theme	energy	757:762	arg1	intensity					684:692	total reflection-infrared intensity	658:692	total reflection-infrared intensity (ATR-IR)	658:701	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	3	15	theme	reflection-infrared	664:682	arg1	EDS					789:791	energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra	757:853	EDS	789:791	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	3	15	theme	reflection-infrared	664:682	arg1	diffraction					592:602	X-ray powder diffraction	579:602	X-ray powder diffraction (XRD)	579:608	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	3	15	theme	reflection-infrared	664:682	arg1	intensity					684:692	total reflection-infrared intensity	658:692	total reflection-infrared intensity (ATR-IR)	658:701	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	3	15	theme	reflection-infrared	664:682	arg1	spectra					847:853	energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra	757:853	spectra	847:853	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	3	15	theme	reflection-infrared	664:682	arg1	measurements					737:748	X-ray photoelectron spectroscopy measurements	704:748	X-ray photoelectron spectroscopy measurements (XPS)	704:754	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	3	15	theme	reflection-infrared	664:682	arg1	ATR-IR					695:700	ATR-IR	695:700	ATR-IR	695:700	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	3	16	theme	light	512:516	arg1	ability					518:524	absorption light ability	501:524	absorption light ability	501:524	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	5	17	dep	supported	974:982	arg1	CA					971:972	CA	971:972	CA	971:972	The CA supported cubic Ag@AgCl catalyst exhibited much higher catalytic activity than octahedral catalyst in terms of the degradation of MO under visible light.
26572362	2	18	theme	exposed	349:355	arg1	facets					373:378	exposed {100} and {111} facets	349:378	exposed {100} and {111} facets	349:378	The Ag@AgCl crystals on electrospun CA composites with exposed {100} and {111} facets were fabricated at room temperature by a double diffusion technique.
26572362	3	19	theme	dispersive	764:773	arg1	EDS					789:791	energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra	757:853	EDS	789:791	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	3	19	theme	dispersive	764:773	arg1	intensity					684:692	total reflection-infrared intensity	658:692	total reflection-infrared intensity (ATR-IR)	658:701	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	0	20	theme	organic	124:130	arg1	dyes					132:135	organic dyes	124:135	organic dyes	124:135	Electrospun cellulose acetate supported Ag@AgCl composites with facet-dependent photocatalytic properties on degradation of organic dyes under visible-light irradiation.
26572362	5	21	theme	higher	1022:1027	arg1	activity					1039:1046	much higher catalytic activity	1017:1046	much higher catalytic activity than octahedral catalyst in terms of the degradation of MO	1017:1105	The CA supported cubic Ag@AgCl catalyst exhibited much higher catalytic activity than octahedral catalyst in terms of the degradation of MO under visible light.
26572362	0	22	theme	AgCl	43:46	arg1	composites					48:57	Ag@AgCl composites	40:57	Ag@AgCl composites with facet-dependent photocatalytic properties	40:104	Electrospun cellulose acetate supported Ag@AgCl composites with facet-dependent photocatalytic properties on degradation of organic dyes under visible-light irradiation.
26572362	3	23	theme	attenuated	647:656	arg1	EDS					789:791	energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra	757:853	EDS	789:791	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	3	23	theme	attenuated	647:656	arg1	diffraction					592:602	X-ray powder diffraction	579:602	X-ray powder diffraction (XRD)	579:608	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	3	23	theme	attenuated	647:656	arg1	intensity					684:692	total reflection-infrared intensity	658:692	total reflection-infrared intensity (ATR-IR)	658:701	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	3	23	theme	attenuated	647:656	arg1	spectra					847:853	energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra	757:853	spectra	847:853	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	3	23	theme	attenuated	647:656	arg1	measurements					737:748	X-ray photoelectron spectroscopy measurements	704:748	X-ray photoelectron spectroscopy measurements (XPS)	704:754	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	3	23	theme	attenuated	647:656	arg1	ATR-IR					695:700	ATR-IR	695:700	ATR-IR	695:700	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	4	24	theme	catalysts	905:913	arg1	activity					889:896	The photocatalytic activity	870:896	The photocatalytic activity of the catalysts	870:913	The photocatalytic activity of the catalysts was evaluated using methyl orange (MO) as a target.
26572362	3	25	theme	reflectance	835:845	arg1	spectra					847:853	energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra	757:853	spectra	847:853	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	3	25	theme	reflectance	835:845	arg1	intensity					684:692	total reflection-infrared intensity	658:692	total reflection-infrared intensity (ATR-IR)	658:701	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	2	26	theme	{	367:367	arg1	}					371:371	{111}	367:371	{111}	367:371	The Ag@AgCl crystals on electrospun CA composites with exposed {100} and {111} facets were fabricated at room temperature by a double diffusion technique.
26572362	0	27	theme	cellulose	12:20	arg1	acetate					22:28	Electrospun cellulose acetate	0:28	Electrospun cellulose acetate	0:28	Electrospun cellulose acetate supported Ag@AgCl composites with facet-dependent photocatalytic properties on degradation of organic dyes under visible-light irradiation.
26572362	3	28	theme	total	658:662	arg1	EDS					789:791	energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra	757:853	EDS	789:791	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	3	28	theme	total	658:662	arg1	diffraction					592:602	X-ray powder diffraction	579:602	X-ray powder diffraction (XRD)	579:608	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	3	28	theme	total	658:662	arg1	intensity					684:692	total reflection-infrared intensity	658:692	total reflection-infrared intensity (ATR-IR)	658:701	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	3	28	theme	total	658:662	arg1	spectra					847:853	energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra	757:853	spectra	847:853	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	3	28	theme	total	658:662	arg1	measurements					737:748	X-ray photoelectron spectroscopy measurements	704:748	X-ray photoelectron spectroscopy measurements (XPS)	704:754	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	3	28	theme	total	658:662	arg1	ATR-IR					695:700	ATR-IR	695:700	ATR-IR	695:700	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	3	29	theme	CA	529:530	arg1	morphology					472:481	morphology	472:481	morphology	472:481	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	3	29	theme	CA	529:530	arg1	structure					461:469	The crystal structure	449:469	The crystal structure	449:469	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	3	29	theme	CA	529:530	arg1	ability					518:524	absorption light ability	501:524	absorption light ability	501:524	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	3	29	theme	CA	529:530	arg1	composition					484:494	composition	484:494	composition	484:494	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	5	30	theme	degradation	1089:1099	arg1	terms					1076:1080	terms	1076:1080	terms of the degradation of MO	1076:1105	The CA supported cubic Ag@AgCl catalyst exhibited much higher catalytic activity than octahedral catalyst in terms of the degradation of MO under visible light.
26572362	6	31	theme	10mg	1132:1135	arg1	cubes					1146:1150	The 10mg CA based cubes	1128:1150	The 10mg CA based cubes	1128:1150	The 10mg CA based cubes could completely degrade MO (10 mg L(-1)) in 160 min.
26572362	0	32	theme	Electrospun	0:10	arg1	acetate					22:28	Electrospun cellulose acetate	0:28	Electrospun cellulose acetate	0:28	Electrospun cellulose acetate supported Ag@AgCl composites with facet-dependent photocatalytic properties on degradation of organic dyes under visible-light irradiation.
26572362	1	33	theme	situ	281:284	arg1	growth					286:291	situ growth	281:291	situ growth	281:291	Electrospun cellulose acetate (CA) membrane was employed as a support that provided sites for AgCl crystals in situ growth.
26572362	5	34	theme	Ag	990:991	arg1	catalyst					998:1005	The CA supported cubic Ag@AgCl catalyst	967:1005	The CA supported cubic Ag@AgCl catalyst	967:1005	The CA supported cubic Ag@AgCl catalyst exhibited much higher catalytic activity than octahedral catalyst in terms of the degradation of MO under visible light.
26572362	2	35	theme	AgCl	301:304	arg1	crystals					306:313	The Ag@AgCl crystals	294:313	The Ag@AgCl crystals on electrospun CA composites with exposed {100} and {111} facets	294:378	The Ag@AgCl crystals on electrospun CA composites with exposed {100} and {111} facets were fabricated at room temperature by a double diffusion technique.
26572362	2	36	theme	}	361:361	arg1	facets					373:378	exposed {100} and {111} facets	349:378	exposed {100} and {111} facets	349:378	The Ag@AgCl crystals on electrospun CA composites with exposed {100} and {111} facets were fabricated at room temperature by a double diffusion technique.
26572362	2	37	theme	room	399:402	arg1	temperature					404:414	room temperature	399:414	room temperature	399:414	The Ag@AgCl crystals on electrospun CA composites with exposed {100} and {111} facets were fabricated at room temperature by a double diffusion technique.
26572362	5	38	theme	MO	1104:1105	arg1	degradation					1089:1099	the degradation	1085:1099	the degradation of MO	1085:1105	The CA supported cubic Ag@AgCl catalyst exhibited much higher catalytic activity than octahedral catalyst in terms of the degradation of MO under visible light.
26572362	1	39	from	crystals	269:276	arg1	growth					286:291	situ growth	281:291	situ growth	281:291	Electrospun cellulose acetate (CA) membrane was employed as a support that provided sites for AgCl crystals in situ growth.
26572362	6	40	theme	CA	1137:1138	arg1	cubes					1146:1150	The 10mg CA based cubes	1128:1150	The 10mg CA based cubes	1128:1150	The 10mg CA based cubes could completely degrade MO (10 mg L(-1)) in 160 min.
26572362	2	41	theme	@	300:300	arg1	crystals					306:313	The Ag@AgCl crystals	294:313	The Ag@AgCl crystals on electrospun CA composites with exposed {100} and {111} facets	294:378	The Ag@AgCl crystals on electrospun CA composites with exposed {100} and {111} facets were fabricated at room temperature by a double diffusion technique.
26572362	0	42	theme	dyes	132:135	arg1	degradation					109:119	degradation	109:119	degradation of organic dyes	109:135	Electrospun cellulose acetate supported Ag@AgCl composites with facet-dependent photocatalytic properties on degradation of organic dyes under visible-light irradiation.
26572362	4	43	theme	photocatalytic	874:887	arg1	activity					889:896	The photocatalytic activity	870:896	The photocatalytic activity of the catalysts	870:913	The photocatalytic activity of the catalysts was evaluated using methyl orange (MO) as a target.
26572362	2	44	theme	Ag	298:299	arg1	crystals					306:313	The Ag@AgCl crystals	294:313	The Ag@AgCl crystals on electrospun CA composites with exposed {100} and {111} facets	294:378	The Ag@AgCl crystals on electrospun CA composites with exposed {100} and {111} facets were fabricated at room temperature by a double diffusion technique.
26572362	0	45	theme	@	42:42	arg1	composites					48:57	Ag@AgCl composites	40:57	Ag@AgCl composites with facet-dependent photocatalytic properties	40:104	Electrospun cellulose acetate supported Ag@AgCl composites with facet-dependent photocatalytic properties on degradation of organic dyes under visible-light irradiation.
26572362	5	46	theme	visible	1113:1119	arg1	light					1121:1125	visible light	1113:1125	visible light	1113:1125	The CA supported cubic Ag@AgCl catalyst exhibited much higher catalytic activity than octahedral catalyst in terms of the degradation of MO under visible light.
26572362	5	47	theme	AgCl	993:996	arg1	catalyst					998:1005	The CA supported cubic Ag@AgCl catalyst	967:1005	The CA supported cubic Ag@AgCl catalyst	967:1005	The CA supported cubic Ag@AgCl catalyst exhibited much higher catalytic activity than octahedral catalyst in terms of the degradation of MO under visible light.
26572362	2	48	theme	diffusion	428:436	arg1	technique					438:446	a double diffusion technique	419:446	a double diffusion technique	419:446	The Ag@AgCl crystals on electrospun CA composites with exposed {100} and {111} facets were fabricated at room temperature by a double diffusion technique.
26572362	3	49	theme	spectroscopy	724:735	arg1	intensity					684:692	total reflection-infrared intensity	658:692	total reflection-infrared intensity (ATR-IR)	658:701	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	3	49	theme	spectroscopy	724:735	arg1	XPS					751:753	XPS	751:753	XPS	751:753	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	3	49	theme	spectroscopy	724:735	arg1	measurements					737:748	X-ray photoelectron spectroscopy measurements	704:748	X-ray photoelectron spectroscopy measurements (XPS)	704:754	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	0	50	theme	Ag	40:41	arg1	composites					48:57	Ag@AgCl composites	40:57	Ag@AgCl composites with facet-dependent photocatalytic properties	40:104	Electrospun cellulose acetate supported Ag@AgCl composites with facet-dependent photocatalytic properties on degradation of organic dyes under visible-light irradiation.
26572362	2	51	with	composites	333:342	arg1	facets					373:378	exposed {100} and {111} facets	349:378	exposed {100} and {111} facets	349:378	The Ag@AgCl crystals on electrospun CA composites with exposed {100} and {111} facets were fabricated at room temperature by a double diffusion technique.
26572362	7	52	theme	catalytic	1247:1255	arg1	ability					1257:1263	a good catalytic ability	1240:1263	a good catalytic ability	1240:1263	The photocatalyst still exhibited a good catalytic ability after three times.
26572362	2	53	theme	}	371:371	arg1	facets					373:378	exposed {100} and {111} facets	349:378	exposed {100} and {111} facets	349:378	The Ag@AgCl crystals on electrospun CA composites with exposed {100} and {111} facets were fabricated at room temperature by a double diffusion technique.
26572362	2	54	theme	double	421:426	arg1	technique					438:446	a double diffusion technique	419:446	a double diffusion technique	419:446	The Ag@AgCl crystals on electrospun CA composites with exposed {100} and {111} facets were fabricated at room temperature by a double diffusion technique.
26572362	3	55	theme	Ag	542:543	arg1	AgCl					545:548	Ag@AgCl	542:548	Ag@AgCl	542:548	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	5	56	theme	catalytic	1029:1037	arg1	activity					1039:1046	much higher catalytic activity	1017:1046	much higher catalytic activity than octahedral catalyst in terms of the degradation of MO	1017:1105	The CA supported cubic Ag@AgCl catalyst exhibited much higher catalytic activity than octahedral catalyst in terms of the degradation of MO under visible light.
26572362	3	57	theme	crystal	453:459	arg1	structure					461:469	The crystal structure	449:469	The crystal structure	449:469	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	0	58	theme	visible-light	143:155	arg1	irradiation					157:167	visible-light irradiation	143:167	visible-light irradiation	143:167	Electrospun cellulose acetate supported Ag@AgCl composites with facet-dependent photocatalytic properties on degradation of organic dyes under visible-light irradiation.
26572362	3	59	theme	X-ray	704:708	arg1	intensity					684:692	total reflection-infrared intensity	658:692	total reflection-infrared intensity (ATR-IR)	658:701	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	3	59	theme	X-ray	704:708	arg1	XPS					751:753	XPS	751:753	XPS	751:753	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	3	59	theme	X-ray	704:708	arg1	measurements					737:748	X-ray photoelectron spectroscopy measurements	704:748	X-ray photoelectron spectroscopy measurements (XPS)	704:754	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	3	60	theme	electron	620:627	arg1	diffraction					592:602	X-ray powder diffraction	579:602	X-ray powder diffraction (XRD)	579:608	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	3	60	theme	electron	620:627	arg1	SEM					641:643	SEM	641:643	SEM	641:643	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	3	60	theme	electron	620:627	arg1	microscopy					629:638	scanning electron microscopy	611:638	scanning electron microscopy (SEM)	611:644	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	3	61	theme	@	544:544	arg1	AgCl					545:548	Ag@AgCl	542:548	Ag@AgCl	542:548	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	2	62	theme	CA	330:331	arg1	composites					333:342	electrospun CA composites	318:342	electrospun CA composites with exposed {100} and {111} facets	318:378	The Ag@AgCl crystals on electrospun CA composites with exposed {100} and {111} facets were fabricated at room temperature by a double diffusion technique.
26572362	3	63	theme	photoelectron	710:722	arg1	intensity					684:692	total reflection-infrared intensity	658:692	total reflection-infrared intensity (ATR-IR)	658:701	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	3	63	theme	photoelectron	710:722	arg1	XPS					751:753	XPS	751:753	XPS	751:753	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	3	63	theme	photoelectron	710:722	arg1	measurements					737:748	X-ray photoelectron spectroscopy measurements	704:748	X-ray photoelectron spectroscopy measurements (XPS)	704:754	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	5	64	theme	supported	974:982	arg1	catalyst					998:1005	The CA supported cubic Ag@AgCl catalyst	967:1005	The CA supported cubic Ag@AgCl catalyst	967:1005	The CA supported cubic Ag@AgCl catalyst exhibited much higher catalytic activity than octahedral catalyst in terms of the degradation of MO under visible light.
26572362	3	65	theme	X-ray	579:583	arg1	XRD					605:607	XRD	605:607	XRD	605:607	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	3	65	theme	X-ray	579:583	arg1	diffraction					592:602	X-ray powder diffraction	579:602	X-ray powder diffraction (XRD)	579:608	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	3	65	theme	X-ray	579:583	arg1	microscopy					629:638	scanning electron microscopy	611:638	scanning electron microscopy (SEM)	611:644	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	3	65	theme	X-ray	579:583	arg1	intensity					684:692	total reflection-infrared intensity	658:692	total reflection-infrared intensity (ATR-IR)	658:701	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	2	66	theme	electrospun	318:328	arg1	composites					333:342	electrospun CA composites	318:342	electrospun CA composites with exposed {100} and {111} facets	318:378	The Ag@AgCl crystals on electrospun CA composites with exposed {100} and {111} facets were fabricated at room temperature by a double diffusion technique.
26572362	3	67	theme	ultraviolet-visible	798:816	arg1	spectra					847:853	energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra	757:853	spectra	847:853	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	3	67	theme	ultraviolet-visible	798:816	arg1	intensity					684:692	total reflection-infrared intensity	658:692	total reflection-infrared intensity (ATR-IR)	658:701	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	4	68	theme	methyl	935:940	arg1	target					959:964	a target	957:964	a target	957:964	The photocatalytic activity of the catalysts was evaluated using methyl orange (MO) as a target.
26572362	4	68	theme	methyl	935:940	arg1	MO					950:951	MO	950:951	MO	950:951	The photocatalytic activity of the catalysts was evaluated using methyl orange (MO) as a target.
26572362	4	68	theme	methyl	935:940	arg1	orange					942:947	methyl orange	935:947	methyl orange (MO)	935:952	The photocatalytic activity of the catalysts was evaluated using methyl orange (MO) as a target.
26572362	5	69	theme	cubic	984:988	arg1	catalyst					998:1005	The CA supported cubic Ag@AgCl catalyst	967:1005	The CA supported cubic Ag@AgCl catalyst	967:1005	The CA supported cubic Ag@AgCl catalyst exhibited much higher catalytic activity than octahedral catalyst in terms of the degradation of MO under visible light.
26572362	3	70	theme	powder	585:590	arg1	XRD					605:607	XRD	605:607	XRD	605:607	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	3	70	theme	powder	585:590	arg1	diffraction					592:602	X-ray powder diffraction	579:602	X-ray powder diffraction (XRD)	579:608	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	3	70	theme	powder	585:590	arg1	microscopy					629:638	scanning electron microscopy	611:638	scanning electron microscopy (SEM)	611:644	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	3	70	theme	powder	585:590	arg1	intensity					684:692	total reflection-infrared intensity	658:692	total reflection-infrared intensity (ATR-IR)	658:701	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	0	71	with	composites	48:57	arg1	properties					95:104	facet-dependent photocatalytic properties	64:104	facet-dependent photocatalytic properties	64:104	Electrospun cellulose acetate supported Ag@AgCl composites with facet-dependent photocatalytic properties on degradation of organic dyes under visible-light irradiation.
26572362	2	72	theme	{	357:357	arg1	}					361:361	{100}	357:361	{100}	357:361	The Ag@AgCl crystals on electrospun CA composites with exposed {100} and {111} facets were fabricated at room temperature by a double diffusion technique.
26572362	0	73	theme	facet-dependent	64:78	arg1	properties					95:104	facet-dependent photocatalytic properties	64:104	facet-dependent photocatalytic properties	64:104	Electrospun cellulose acetate supported Ag@AgCl composites with facet-dependent photocatalytic properties on degradation of organic dyes under visible-light irradiation.
26572362	3	74	theme	scanning	611:618	arg1	diffraction					592:602	X-ray powder diffraction	579:602	X-ray powder diffraction (XRD)	579:608	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	3	74	theme	scanning	611:618	arg1	SEM					641:643	SEM	641:643	SEM	641:643	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	3	74	theme	scanning	611:618	arg1	microscopy					629:638	scanning electron microscopy	611:638	scanning electron microscopy (SEM)	611:644	The crystal structure, morphology, composition, and absorption light ability of CA supported Ag@AgCl were characterized utilizing X-ray powder diffraction (XRD), scanning electron microscopy (SEM), attenuated total reflection-infrared intensity (ATR-IR), X-ray photoelectron spectroscopy measurements (XPS), energy dispersive spectrometer (EDS) and ultraviolet-visible (UV-vis) diffuse reflectance spectra, respectively.
26572362	5	75	from	activity	1039:1046	arg1	terms					1076:1080	terms	1076:1080	terms of the degradation of MO	1076:1105	The CA supported cubic Ag@AgCl catalyst exhibited much higher catalytic activity than octahedral catalyst in terms of the degradation of MO under visible light.
26572362	1	76	theme	Electrospun	170:180	arg1	CA					201:202	CA	201:202	CA	201:202	Electrospun cellulose acetate (CA) membrane was employed as a support that provided sites for AgCl crystals in situ growth.
26572362	1	76	theme	Electrospun	170:180	arg1	acetate					192:198	Electrospun cellulose acetate	170:198	Electrospun cellulose acetate (CA) membrane	170:212	Electrospun cellulose acetate (CA) membrane was employed as a support that provided sites for AgCl crystals in situ growth.
24254641	0	0	theme	prostate	88:95	arg1	cancer					97:102	prostate cancer	88:102	prostate cancer	88:102	Capillary electrophoresis of urinary prostate glycoproteins assists in the diagnosis of prostate cancer.
24254641	6	1	theme	urinary	1217:1223	arg1	marker					1238:1243	the urinary glycoprofile marker	1213:1243	the urinary glycoprofile marker	1213:1243	Prostate-specific antigen (PSA) and the urinary glycoprofile marker showed comparable overall receiver operating characteristic curve analysis as well as in the diagnostic gray zone with serum PSA values between 4 and 10 μg/L. However, when combining PSA and the urinary glycoprofile marker, the latter gave an additive diagnostic value to serum PSA (p ≤ 0.001).
24254641	3	2	theme	prostate	497:504	arg1	hyperplasia					506:516	benign prostate hyperplasia	490:516	benign prostate hyperplasia (BPH; n = 62)	490:530	We determined the urinary asparagine-linked glycan (N-glycan) profile of prostatic proteins of healthy volunteers (n = 25), patients with benign prostate hyperplasia (BPH; n = 62) and newly diagnosed PCa patients (n = 42) using DNA-sequencer-assisted fluorophore-assisted carbohydrate electrophoresis.
24254641	8	3	theme	new	1451:1453	arg1	biomarker					1455:1463	a new biomarker	1449:1463	a new biomarker for PCa	1449:1471	These changes could lead to the discovery of a new biomarker for PCa.
24254641	1	4	theme	prostate	161:168	arg1	PCa					178:180	PCa	178:180	PCa	178:180	Prostate marker assays are widely used for detection of prostate cancer (PCa) but are associated with considerable sensitivity and specificity problems.
24254641	1	4	theme	prostate	161:168	arg1	cancer					170:175	prostate cancer	161:175	prostate cancer (PCa)	161:181	Prostate marker assays are widely used for detection of prostate cancer (PCa) but are associated with considerable sensitivity and specificity problems.
24254641	5	5	theme	overall	920:926	arg1	0.026					946:950	0.026	946:950	0.026	946:950	N-glycans of PCa compared to BPH were characterized by a significant decrease in triantennary structures (p = 0.047) and overall fucosylation (p = 0.026).
24254641	5	5	theme	overall	920:926	arg1	fucosylation					928:939	overall fucosylation	920:939	overall fucosylation (p = 0.026)	920:951	N-glycans of PCa compared to BPH were characterized by a significant decrease in triantennary structures (p = 0.047) and overall fucosylation (p = 0.026).
24254641	3	6	theme	urinary	370:376	arg1	profile					414:420	the urinary asparagine-linked glycan (N-glycan) profile	366:420	the urinary asparagine-linked glycan (N-glycan) profile of prostatic proteins of healthy volunteers (n = 25), patients with benign prostate hyperplasia (BPH; n = 62) and newly diagnosed PCa patients (n = 42)	366:572	We determined the urinary asparagine-linked glycan (N-glycan) profile of prostatic proteins of healthy volunteers (n = 25), patients with benign prostate hyperplasia (BPH; n = 62) and newly diagnosed PCa patients (n = 42) using DNA-sequencer-assisted fluorophore-assisted carbohydrate electrophoresis.
24254641	4	7	theme	N-glycan	778:785	arg1	structures					787:796	N-glycan structures	778:796	N-glycan structures	778:796	Through squeezing of the prostate, a sufficient amount of prostatic proteins was obtained for direct structural analyses of N-glycan structures.
24254641	2	8	theme	protein	295:301	arg1	profiles					317:324	prostatic protein glycosylation profiles	285:324	prostatic protein glycosylation profiles	285:324	Therefore, we investigated prostatic protein glycosylation profiles as a potential biomarker.
24254641	2	8	theme	protein	295:301	arg1	biomarker					341:349	a potential biomarker	329:349	a potential biomarker	329:349	Therefore, we investigated prostatic protein glycosylation profiles as a potential biomarker.
24254641	2	9	theme	glycosylation	303:315	arg1	profiles					317:324	prostatic protein glycosylation profiles	285:324	prostatic protein glycosylation profiles	285:324	Therefore, we investigated prostatic protein glycosylation profiles as a potential biomarker.
24254641	2	9	theme	glycosylation	303:315	arg1	biomarker					341:349	a potential biomarker	329:349	a potential biomarker	329:349	Therefore, we investigated prostatic protein glycosylation profiles as a potential biomarker.
24254641	0	10	theme	cancer	97:102	arg1	diagnosis					75:83	the diagnosis	71:83	the diagnosis of prostate cancer	71:102	Capillary electrophoresis of urinary prostate glycoproteins assists in the diagnosis of prostate cancer.
24254641	5	11	theme	PCa	812:814	arg1	N-glycans					799:807	N-glycans	799:807	N-glycans of PCa compared to BPH	799:830	N-glycans of PCa compared to BPH were characterized by a significant decrease in triantennary structures (p = 0.047) and overall fucosylation (p = 0.026).
24254641	4	12	theme	direct	748:753	arg1	analyses					766:773	direct structural analyses	748:773	direct structural analyses of N-glycan structures	748:796	Through squeezing of the prostate, a sufficient amount of prostatic proteins was obtained for direct structural analyses of N-glycan structures.
24254641	2	13	theme	prostatic	285:293	arg1	profiles					317:324	prostatic protein glycosylation profiles	285:324	prostatic protein glycosylation profiles	285:324	Therefore, we investigated prostatic protein glycosylation profiles as a potential biomarker.
24254641	2	13	theme	prostatic	285:293	arg1	biomarker					341:349	a potential biomarker	329:349	a potential biomarker	329:349	Therefore, we investigated prostatic protein glycosylation profiles as a potential biomarker.
24254641	3	14	theme	patients	476:483	arg1	proteins					435:442	prostatic proteins	425:442	prostatic proteins of healthy volunteers (n = 25), patients with benign prostate hyperplasia (BPH; n = 62) and newly diagnosed PCa patients (n = 42)	425:572	We determined the urinary asparagine-linked glycan (N-glycan) profile of prostatic proteins of healthy volunteers (n = 25), patients with benign prostate hyperplasia (BPH; n = 62) and newly diagnosed PCa patients (n = 42) using DNA-sequencer-assisted fluorophore-assisted carbohydrate electrophoresis.
24254641	6	15	theme	p	1305:1305	arg1	≤					1307:1307	p ≤ 0.001	1305:1313	p ≤ 0.001	1305:1313	Prostate-specific antigen (PSA) and the urinary glycoprofile marker showed comparable overall receiver operating characteristic curve analysis as well as in the diagnostic gray zone with serum PSA values between 4 and 10 μg/L. However, when combining PSA and the urinary glycoprofile marker, the latter gave an additive diagnostic value to serum PSA (p ≤ 0.001).
24254641	6	15	theme	p	1305:1305	arg1	PSA					1300:1302	serum PSA	1294:1302	serum PSA (p ≤ 0.001)	1294:1314	Prostate-specific antigen (PSA) and the urinary glycoprofile marker showed comparable overall receiver operating characteristic curve analysis as well as in the diagnostic gray zone with serum PSA values between 4 and 10 μg/L. However, when combining PSA and the urinary glycoprofile marker, the latter gave an additive diagnostic value to serum PSA (p ≤ 0.001).
24254641	6	16	theme	diagnostic	1115:1124	arg1	zone					1131:1134	the diagnostic gray zone	1111:1134	the diagnostic gray zone with serum PSA values between 4 and 10 μg/L.	1111:1179	Prostate-specific antigen (PSA) and the urinary glycoprofile marker showed comparable overall receiver operating characteristic curve analysis as well as in the diagnostic gray zone with serum PSA values between 4 and 10 μg/L. However, when combining PSA and the urinary glycoprofile marker, the latter gave an additive diagnostic value to serum PSA (p ≤ 0.001).
24254641	6	17	theme	glycoprofile	1225:1236	arg1	marker					1238:1243	the urinary glycoprofile marker	1213:1243	the urinary glycoprofile marker	1213:1243	Prostate-specific antigen (PSA) and the urinary glycoprofile marker showed comparable overall receiver operating characteristic curve analysis as well as in the diagnostic gray zone with serum PSA values between 4 and 10 μg/L. However, when combining PSA and the urinary glycoprofile marker, the latter gave an additive diagnostic value to serum PSA (p ≤ 0.001).
24254641	6	18	theme	characteristic	1067:1080	arg1	analysis					1088:1095	characteristic curve analysis	1067:1095	characteristic curve analysis	1067:1095	Prostate-specific antigen (PSA) and the urinary glycoprofile marker showed comparable overall receiver operating characteristic curve analysis as well as in the diagnostic gray zone with serum PSA values between 4 and 10 μg/L. However, when combining PSA and the urinary glycoprofile marker, the latter gave an additive diagnostic value to serum PSA (p ≤ 0.001).
24254641	1	19	theme	cancer	170:175	arg1	detection					148:156	detection	148:156	detection of prostate cancer (PCa)	148:181	Prostate marker assays are widely used for detection of prostate cancer (PCa) but are associated with considerable sensitivity and specificity problems.
24254641	6	20	theme	Prostate-specific	954:970	arg1	PSA					981:983	PSA	981:983	PSA	981:983	Prostate-specific antigen (PSA) and the urinary glycoprofile marker showed comparable overall receiver operating characteristic curve analysis as well as in the diagnostic gray zone with serum PSA values between 4 and 10 μg/L. However, when combining PSA and the urinary glycoprofile marker, the latter gave an additive diagnostic value to serum PSA (p ≤ 0.001).
24254641	6	20	theme	Prostate-specific	954:970	arg1	antigen					972:978	Prostate-specific antigen	954:978	Prostate-specific antigen (PSA)	954:984	Prostate-specific antigen (PSA) and the urinary glycoprofile marker showed comparable overall receiver operating characteristic curve analysis as well as in the diagnostic gray zone with serum PSA values between 4 and 10 μg/L. However, when combining PSA and the urinary glycoprofile marker, the latter gave an additive diagnostic value to serum PSA (p ≤ 0.001).
24254641	5	21	theme	p	905:905	arg1	0.047					909:913	p = 0.047	905:913	p = 0.047	905:913	N-glycans of PCa compared to BPH were characterized by a significant decrease in triantennary structures (p = 0.047) and overall fucosylation (p = 0.026).
24254641	5	21	theme	p	905:905	arg1	structures					893:902	triantennary structures	880:902	triantennary structures (p = 0.047)	880:914	N-glycans of PCa compared to BPH were characterized by a significant decrease in triantennary structures (p = 0.047) and overall fucosylation (p = 0.026).
24254641	3	22	theme	prostatic	425:433	arg1	proteins					435:442	prostatic proteins	425:442	prostatic proteins of healthy volunteers (n = 25), patients with benign prostate hyperplasia (BPH; n = 62) and newly diagnosed PCa patients (n = 42)	425:572	We determined the urinary asparagine-linked glycan (N-glycan) profile of prostatic proteins of healthy volunteers (n = 25), patients with benign prostate hyperplasia (BPH; n = 62) and newly diagnosed PCa patients (n = 42) using DNA-sequencer-assisted fluorophore-assisted carbohydrate electrophoresis.
24254641	3	23	link	asparagine-linked	378:394	arg1	profile					414:420	the urinary asparagine-linked glycan (N-glycan) profile	366:420	the urinary asparagine-linked glycan (N-glycan) profile of prostatic proteins of healthy volunteers (n = 25), patients with benign prostate hyperplasia (BPH; n = 62) and newly diagnosed PCa patients (n = 42)	366:572	We determined the urinary asparagine-linked glycan (N-glycan) profile of prostatic proteins of healthy volunteers (n = 25), patients with benign prostate hyperplasia (BPH; n = 62) and newly diagnosed PCa patients (n = 42) using DNA-sequencer-assisted fluorophore-assisted carbohydrate electrophoresis.
24254641	6	24	theme	curve	1082:1086	arg1	analysis					1088:1095	characteristic curve analysis	1067:1095	characteristic curve analysis	1067:1095	Prostate-specific antigen (PSA) and the urinary glycoprofile marker showed comparable overall receiver operating characteristic curve analysis as well as in the diagnostic gray zone with serum PSA values between 4 and 10 μg/L. However, when combining PSA and the urinary glycoprofile marker, the latter gave an additive diagnostic value to serum PSA (p ≤ 0.001).
24254641	5	25	theme	significant	856:866	arg1	decrease					868:875	a significant decrease	854:875	a significant decrease in triantennary structures (p = 0.047) and overall fucosylation (p = 0.026)	854:951	N-glycans of PCa compared to BPH were characterized by a significant decrease in triantennary structures (p = 0.047) and overall fucosylation (p = 0.026).
24254641	3	26	theme	N-glycan	404:411	arg1	profile					414:420	the urinary asparagine-linked glycan (N-glycan) profile	366:420	the urinary asparagine-linked glycan (N-glycan) profile of prostatic proteins of healthy volunteers (n = 25), patients with benign prostate hyperplasia (BPH; n = 62) and newly diagnosed PCa patients (n = 42)	366:572	We determined the urinary asparagine-linked glycan (N-glycan) profile of prostatic proteins of healthy volunteers (n = 25), patients with benign prostate hyperplasia (BPH; n = 62) and newly diagnosed PCa patients (n = 42) using DNA-sequencer-assisted fluorophore-assisted carbohydrate electrophoresis.
24254641	6	27	theme	serum	1141:1145	arg1	values					1151:1156	serum PSA values	1141:1156	serum PSA values	1141:1156	Prostate-specific antigen (PSA) and the urinary glycoprofile marker showed comparable overall receiver operating characteristic curve analysis as well as in the diagnostic gray zone with serum PSA values between 4 and 10 μg/L. However, when combining PSA and the urinary glycoprofile marker, the latter gave an additive diagnostic value to serum PSA (p ≤ 0.001).
24254641	1	28	used	used	139:142	arg2	assays					121:126	Prostate marker assays	105:126	Prostate marker assays	105:126	Prostate marker assays are widely used for detection of prostate cancer (PCa) but are associated with considerable sensitivity and specificity problems.
24254641	8	29	theme	biomarker	1455:1463	arg1	discovery					1436:1444	the discovery	1432:1444	the discovery of a new biomarker for PCa	1432:1471	These changes could lead to the discovery of a new biomarker for PCa.
24254641	0	30	theme	Capillary	0:8	arg1	electrophoresis					10:24	Capillary electrophoresis	0:24	Capillary electrophoresis of urinary prostate glycoproteins	0:58	Capillary electrophoresis of urinary prostate glycoproteins assists in the diagnosis of prostate cancer.
24254641	3	31	theme	diagnosed	542:550	arg1	n					566:566	n = 42	566:571	n = 42	566:571	We determined the urinary asparagine-linked glycan (N-glycan) profile of prostatic proteins of healthy volunteers (n = 25), patients with benign prostate hyperplasia (BPH; n = 62) and newly diagnosed PCa patients (n = 42) using DNA-sequencer-assisted fluorophore-assisted carbohydrate electrophoresis.
24254641	3	31	theme	diagnosed	542:550	arg1	patients					556:563	newly diagnosed PCa patients	536:563	newly diagnosed PCa patients (n = 42)	536:572	We determined the urinary asparagine-linked glycan (N-glycan) profile of prostatic proteins of healthy volunteers (n = 25), patients with benign prostate hyperplasia (BPH; n = 62) and newly diagnosed PCa patients (n = 42) using DNA-sequencer-assisted fluorophore-assisted carbohydrate electrophoresis.
24254641	4	32	theme	structural	755:764	arg1	analyses					766:773	direct structural analyses	748:773	direct structural analyses of N-glycan structures	748:796	Through squeezing of the prostate, a sufficient amount of prostatic proteins was obtained for direct structural analyses of N-glycan structures.
24254641	3	33	theme	healthy	447:453	arg1	volunteers					455:464	healthy volunteers	447:464	healthy volunteers (n = 25)	447:473	We determined the urinary asparagine-linked glycan (N-glycan) profile of prostatic proteins of healthy volunteers (n = 25), patients with benign prostate hyperplasia (BPH; n = 62) and newly diagnosed PCa patients (n = 42) using DNA-sequencer-assisted fluorophore-assisted carbohydrate electrophoresis.
24254641	3	33	theme	healthy	447:453	arg1	n					467:467	n = 25	467:472	n = 25	467:472	We determined the urinary asparagine-linked glycan (N-glycan) profile of prostatic proteins of healthy volunteers (n = 25), patients with benign prostate hyperplasia (BPH; n = 62) and newly diagnosed PCa patients (n = 42) using DNA-sequencer-assisted fluorophore-assisted carbohydrate electrophoresis.
24254641	0	34	theme	urinary	29:35	arg1	glycoproteins					46:58	urinary prostate glycoproteins	29:58	urinary prostate glycoproteins	29:58	Capillary electrophoresis of urinary prostate glycoproteins assists in the diagnosis of prostate cancer.
24254641	4	35	theme	proteins	722:729	arg1	amount					702:707	a sufficient amount	689:707	a sufficient amount of prostatic proteins	689:729	Through squeezing of the prostate, a sufficient amount of prostatic proteins was obtained for direct structural analyses of N-glycan structures.
24254641	4	35	theme	proteins	722:729	arg1	proteins					722:729	prostatic proteins	712:729	prostatic proteins	712:729	Through squeezing of the prostate, a sufficient amount of prostatic proteins was obtained for direct structural analyses of N-glycan structures.
24254641	1	36	theme	Prostate	105:112	arg1	assays					121:126	Prostate marker assays	105:126	Prostate marker assays	105:126	Prostate marker assays are widely used for detection of prostate cancer (PCa) but are associated with considerable sensitivity and specificity problems.
24254641	3	37	with	patients	476:483	arg1	hyperplasia					506:516	benign prostate hyperplasia	490:516	benign prostate hyperplasia (BPH; n = 62)	490:530	We determined the urinary asparagine-linked glycan (N-glycan) profile of prostatic proteins of healthy volunteers (n = 25), patients with benign prostate hyperplasia (BPH; n = 62) and newly diagnosed PCa patients (n = 42) using DNA-sequencer-assisted fluorophore-assisted carbohydrate electrophoresis.
24254641	6	38	theme	comparable	1029:1038	arg1	receiver					1048:1055	comparable overall receiver	1029:1055	comparable overall receiver operating characteristic curve analysis	1029:1095	Prostate-specific antigen (PSA) and the urinary glycoprofile marker showed comparable overall receiver operating characteristic curve analysis as well as in the diagnostic gray zone with serum PSA values between 4 and 10 μg/L. However, when combining PSA and the urinary glycoprofile marker, the latter gave an additive diagnostic value to serum PSA (p ≤ 0.001).
24254641	3	39	dep	hyperplasia	506:516	arg1	=					526:526	=	526:526	=	526:526	We determined the urinary asparagine-linked glycan (N-glycan) profile of prostatic proteins of healthy volunteers (n = 25), patients with benign prostate hyperplasia (BPH; n = 62) and newly diagnosed PCa patients (n = 42) using DNA-sequencer-assisted fluorophore-assisted carbohydrate electrophoresis.
24254641	6	40	theme	serum	1294:1298	arg1	≤					1307:1307	p ≤ 0.001	1305:1313	p ≤ 0.001	1305:1313	Prostate-specific antigen (PSA) and the urinary glycoprofile marker showed comparable overall receiver operating characteristic curve analysis as well as in the diagnostic gray zone with serum PSA values between 4 and 10 μg/L. However, when combining PSA and the urinary glycoprofile marker, the latter gave an additive diagnostic value to serum PSA (p ≤ 0.001).
24254641	6	40	theme	serum	1294:1298	arg1	PSA					1300:1302	serum PSA	1294:1302	serum PSA (p ≤ 0.001)	1294:1314	Prostate-specific antigen (PSA) and the urinary glycoprofile marker showed comparable overall receiver operating characteristic curve analysis as well as in the diagnostic gray zone with serum PSA values between 4 and 10 μg/L. However, when combining PSA and the urinary glycoprofile marker, the latter gave an additive diagnostic value to serum PSA (p ≤ 0.001).
24254641	3	41	theme	carbohydrate	624:635	arg1	electrophoresis					637:651	DNA-sequencer-assisted fluorophore-assisted carbohydrate electrophoresis	580:651	DNA-sequencer-assisted fluorophore-assisted carbohydrate electrophoresis	580:651	We determined the urinary asparagine-linked glycan (N-glycan) profile of prostatic proteins of healthy volunteers (n = 25), patients with benign prostate hyperplasia (BPH; n = 62) and newly diagnosed PCa patients (n = 42) using DNA-sequencer-assisted fluorophore-assisted carbohydrate electrophoresis.
24254641	1	42	theme	marker	114:119	arg1	assays					121:126	Prostate marker assays	105:126	Prostate marker assays	105:126	Prostate marker assays are widely used for detection of prostate cancer (PCa) but are associated with considerable sensitivity and specificity problems.
24254641	5	43	theme	triantennary	880:891	arg1	0.047					909:913	p = 0.047	905:913	p = 0.047	905:913	N-glycans of PCa compared to BPH were characterized by a significant decrease in triantennary structures (p = 0.047) and overall fucosylation (p = 0.026).
24254641	5	43	theme	triantennary	880:891	arg1	structures					893:902	triantennary structures	880:902	triantennary structures (p = 0.047)	880:914	N-glycans of PCa compared to BPH were characterized by a significant decrease in triantennary structures (p = 0.047) and overall fucosylation (p = 0.026).
24254641	0	44	theme	glycoproteins	46:58	arg1	electrophoresis					10:24	Capillary electrophoresis	0:24	Capillary electrophoresis of urinary prostate glycoproteins	0:58	Capillary electrophoresis of urinary prostate glycoproteins assists in the diagnosis of prostate cancer.
24254641	4	45	theme	sufficient	691:700	arg1	amount					702:707	a sufficient amount	689:707	a sufficient amount of prostatic proteins	689:729	Through squeezing of the prostate, a sufficient amount of prostatic proteins was obtained for direct structural analyses of N-glycan structures.
24254641	4	45	theme	sufficient	691:700	arg1	proteins					722:729	prostatic proteins	712:729	prostatic proteins	712:729	Through squeezing of the prostate, a sufficient amount of prostatic proteins was obtained for direct structural analyses of N-glycan structures.
24254641	6	46	theme	overall	1040:1046	arg1	receiver					1048:1055	comparable overall receiver	1029:1055	comparable overall receiver operating characteristic curve analysis	1029:1095	Prostate-specific antigen (PSA) and the urinary glycoprofile marker showed comparable overall receiver operating characteristic curve analysis as well as in the diagnostic gray zone with serum PSA values between 4 and 10 μg/L. However, when combining PSA and the urinary glycoprofile marker, the latter gave an additive diagnostic value to serum PSA (p ≤ 0.001).
24254641	0	47	theme	prostate	37:44	arg1	glycoproteins					46:58	urinary prostate glycoproteins	29:58	urinary prostate glycoproteins	29:58	Capillary electrophoresis of urinary prostate glycoproteins assists in the diagnosis of prostate cancer.
24254641	6	48	with	zone	1131:1134	arg1	values					1151:1156	serum PSA values	1141:1156	serum PSA values	1141:1156	Prostate-specific antigen (PSA) and the urinary glycoprofile marker showed comparable overall receiver operating characteristic curve analysis as well as in the diagnostic gray zone with serum PSA values between 4 and 10 μg/L. However, when combining PSA and the urinary glycoprofile marker, the latter gave an additive diagnostic value to serum PSA (p ≤ 0.001).
24254641	3	49	theme	proteins	435:442	arg1	profile					414:420	the urinary asparagine-linked glycan (N-glycan) profile	366:420	the urinary asparagine-linked glycan (N-glycan) profile of prostatic proteins of healthy volunteers (n = 25), patients with benign prostate hyperplasia (BPH; n = 62) and newly diagnosed PCa patients (n = 42)	366:572	We determined the urinary asparagine-linked glycan (N-glycan) profile of prostatic proteins of healthy volunteers (n = 25), patients with benign prostate hyperplasia (BPH; n = 62) and newly diagnosed PCa patients (n = 42) using DNA-sequencer-assisted fluorophore-assisted carbohydrate electrophoresis.
24254641	3	50	theme	DNA-sequencer-assisted	580:601	arg1	electrophoresis					637:651	DNA-sequencer-assisted fluorophore-assisted carbohydrate electrophoresis	580:651	DNA-sequencer-assisted fluorophore-assisted carbohydrate electrophoresis	580:651	We determined the urinary asparagine-linked glycan (N-glycan) profile of prostatic proteins of healthy volunteers (n = 25), patients with benign prostate hyperplasia (BPH; n = 62) and newly diagnosed PCa patients (n = 42) using DNA-sequencer-assisted fluorophore-assisted carbohydrate electrophoresis.
24254641	3	51	theme	glycan	396:401	arg1	profile					414:420	the urinary asparagine-linked glycan (N-glycan) profile	366:420	the urinary asparagine-linked glycan (N-glycan) profile of prostatic proteins of healthy volunteers (n = 25), patients with benign prostate hyperplasia (BPH; n = 62) and newly diagnosed PCa patients (n = 42)	366:572	We determined the urinary asparagine-linked glycan (N-glycan) profile of prostatic proteins of healthy volunteers (n = 25), patients with benign prostate hyperplasia (BPH; n = 62) and newly diagnosed PCa patients (n = 42) using DNA-sequencer-assisted fluorophore-assisted carbohydrate electrophoresis.
24254641	3	52	theme	=	469:469	arg1	volunteers					455:464	healthy volunteers	447:464	healthy volunteers (n = 25)	447:473	We determined the urinary asparagine-linked glycan (N-glycan) profile of prostatic proteins of healthy volunteers (n = 25), patients with benign prostate hyperplasia (BPH; n = 62) and newly diagnosed PCa patients (n = 42) using DNA-sequencer-assisted fluorophore-assisted carbohydrate electrophoresis.
24254641	3	52	theme	=	469:469	arg1	n					467:467	n = 25	467:472	n = 25	467:472	We determined the urinary asparagine-linked glycan (N-glycan) profile of prostatic proteins of healthy volunteers (n = 25), patients with benign prostate hyperplasia (BPH; n = 62) and newly diagnosed PCa patients (n = 42) using DNA-sequencer-assisted fluorophore-assisted carbohydrate electrophoresis.
24254641	6	53	theme	diagnostic	1274:1283	arg1	value					1285:1289	an additive diagnostic value	1262:1289	an additive diagnostic value	1262:1289	Prostate-specific antigen (PSA) and the urinary glycoprofile marker showed comparable overall receiver operating characteristic curve analysis as well as in the diagnostic gray zone with serum PSA values between 4 and 10 μg/L. However, when combining PSA and the urinary glycoprofile marker, the latter gave an additive diagnostic value to serum PSA (p ≤ 0.001).
24254641	1	54	dep	sensitivity	220:230	arg1	problems					248:255	problems	248:255	problems	248:255	Prostate marker assays are widely used for detection of prostate cancer (PCa) but are associated with considerable sensitivity and specificity problems.
24254641	3	55	theme	PCa	552:554	arg1	n					566:566	n = 42	566:571	n = 42	566:571	We determined the urinary asparagine-linked glycan (N-glycan) profile of prostatic proteins of healthy volunteers (n = 25), patients with benign prostate hyperplasia (BPH; n = 62) and newly diagnosed PCa patients (n = 42) using DNA-sequencer-assisted fluorophore-assisted carbohydrate electrophoresis.
24254641	3	55	theme	PCa	552:554	arg1	patients					556:563	newly diagnosed PCa patients	536:563	newly diagnosed PCa patients (n = 42)	536:572	We determined the urinary asparagine-linked glycan (N-glycan) profile of prostatic proteins of healthy volunteers (n = 25), patients with benign prostate hyperplasia (BPH; n = 62) and newly diagnosed PCa patients (n = 42) using DNA-sequencer-assisted fluorophore-assisted carbohydrate electrophoresis.
24254641	5	56	theme	=	907:907	arg1	0.047					909:913	p = 0.047	905:913	p = 0.047	905:913	N-glycans of PCa compared to BPH were characterized by a significant decrease in triantennary structures (p = 0.047) and overall fucosylation (p = 0.026).
24254641	5	56	theme	=	907:907	arg1	structures					893:902	triantennary structures	880:902	triantennary structures (p = 0.047)	880:914	N-glycans of PCa compared to BPH were characterized by a significant decrease in triantennary structures (p = 0.047) and overall fucosylation (p = 0.026).
24254641	6	57	theme	additive	1265:1272	arg1	value					1285:1289	an additive diagnostic value	1262:1289	an additive diagnostic value	1262:1289	Prostate-specific antigen (PSA) and the urinary glycoprofile marker showed comparable overall receiver operating characteristic curve analysis as well as in the diagnostic gray zone with serum PSA values between 4 and 10 μg/L. However, when combining PSA and the urinary glycoprofile marker, the latter gave an additive diagnostic value to serum PSA (p ≤ 0.001).
24254641	3	58	theme	fluorophore-assisted	603:622	arg1	electrophoresis					637:651	DNA-sequencer-assisted fluorophore-assisted carbohydrate electrophoresis	580:651	DNA-sequencer-assisted fluorophore-assisted carbohydrate electrophoresis	580:651	We determined the urinary asparagine-linked glycan (N-glycan) profile of prostatic proteins of healthy volunteers (n = 25), patients with benign prostate hyperplasia (BPH; n = 62) and newly diagnosed PCa patients (n = 42) using DNA-sequencer-assisted fluorophore-assisted carbohydrate electrophoresis.
24254641	3	59	theme	patients	556:563	arg1	proteins					435:442	prostatic proteins	425:442	prostatic proteins of healthy volunteers (n = 25), patients with benign prostate hyperplasia (BPH; n = 62) and newly diagnosed PCa patients (n = 42)	425:572	We determined the urinary asparagine-linked glycan (N-glycan) profile of prostatic proteins of healthy volunteers (n = 25), patients with benign prostate hyperplasia (BPH; n = 62) and newly diagnosed PCa patients (n = 42) using DNA-sequencer-assisted fluorophore-assisted carbohydrate electrophoresis.
24254641	3	60	with	patients	556:563	arg1	hyperplasia					506:516	benign prostate hyperplasia	490:516	benign prostate hyperplasia (BPH; n = 62)	490:530	We determined the urinary asparagine-linked glycan (N-glycan) profile of prostatic proteins of healthy volunteers (n = 25), patients with benign prostate hyperplasia (BPH; n = 62) and newly diagnosed PCa patients (n = 42) using DNA-sequencer-assisted fluorophore-assisted carbohydrate electrophoresis.
24254641	3	61	theme	benign	490:495	arg1	hyperplasia					506:516	benign prostate hyperplasia	490:516	benign prostate hyperplasia (BPH; n = 62)	490:530	We determined the urinary asparagine-linked glycan (N-glycan) profile of prostatic proteins of healthy volunteers (n = 25), patients with benign prostate hyperplasia (BPH; n = 62) and newly diagnosed PCa patients (n = 42) using DNA-sequencer-assisted fluorophore-assisted carbohydrate electrophoresis.
24254641	3	62	theme	volunteers	455:464	arg1	proteins					435:442	prostatic proteins	425:442	prostatic proteins of healthy volunteers (n = 25), patients with benign prostate hyperplasia (BPH; n = 62) and newly diagnosed PCa patients (n = 42)	425:572	We determined the urinary asparagine-linked glycan (N-glycan) profile of prostatic proteins of healthy volunteers (n = 25), patients with benign prostate hyperplasia (BPH; n = 62) and newly diagnosed PCa patients (n = 42) using DNA-sequencer-assisted fluorophore-assisted carbohydrate electrophoresis.
24254641	3	63	with	volunteers	455:464	arg1	hyperplasia					506:516	benign prostate hyperplasia	490:516	benign prostate hyperplasia (BPH; n = 62)	490:530	We determined the urinary asparagine-linked glycan (N-glycan) profile of prostatic proteins of healthy volunteers (n = 25), patients with benign prostate hyperplasia (BPH; n = 62) and newly diagnosed PCa patients (n = 42) using DNA-sequencer-assisted fluorophore-assisted carbohydrate electrophoresis.
24254641	4	64	theme	structures	787:796	arg1	analyses					766:773	direct structural analyses	748:773	direct structural analyses of N-glycan structures	748:796	Through squeezing of the prostate, a sufficient amount of prostatic proteins was obtained for direct structural analyses of N-glycan structures.
24254641	3	65	dep	=	526:526	arg1	BPH					519:521	BPH	519:521	BPH	519:521	We determined the urinary asparagine-linked glycan (N-glycan) profile of prostatic proteins of healthy volunteers (n = 25), patients with benign prostate hyperplasia (BPH; n = 62) and newly diagnosed PCa patients (n = 42) using DNA-sequencer-assisted fluorophore-assisted carbohydrate electrophoresis.
24254641	1	66	theme	considerable	207:218	arg1	sensitivity					220:230	considerable sensitivity	207:230	considerable sensitivity	207:230	Prostate marker assays are widely used for detection of prostate cancer (PCa) but are associated with considerable sensitivity and specificity problems.
24254641	6	67	theme	PSA	1147:1149	arg1	values					1151:1156	serum PSA values	1141:1156	serum PSA values	1141:1156	Prostate-specific antigen (PSA) and the urinary glycoprofile marker showed comparable overall receiver operating characteristic curve analysis as well as in the diagnostic gray zone with serum PSA values between 4 and 10 μg/L. However, when combining PSA and the urinary glycoprofile marker, the latter gave an additive diagnostic value to serum PSA (p ≤ 0.001).
24254641	0	68	gly	glycoproteins	46:58	arg1	glycoproteins					46:58	urinary prostate glycoproteins	29:58	urinary prostate glycoproteins	29:58	Capillary electrophoresis of urinary prostate glycoproteins assists in the diagnosis of prostate cancer.
24254641	5	69	from	decrease	868:875	arg1	0.026					946:950	0.026	946:950	0.026	946:950	N-glycans of PCa compared to BPH were characterized by a significant decrease in triantennary structures (p = 0.047) and overall fucosylation (p = 0.026).
24254641	5	69	from	decrease	868:875	arg1	0.047					909:913	p = 0.047	905:913	p = 0.047	905:913	N-glycans of PCa compared to BPH were characterized by a significant decrease in triantennary structures (p = 0.047) and overall fucosylation (p = 0.026).
24254641	5	69	from	decrease	868:875	arg1	fucosylation					928:939	overall fucosylation	920:939	overall fucosylation (p = 0.026)	920:951	N-glycans of PCa compared to BPH were characterized by a significant decrease in triantennary structures (p = 0.047) and overall fucosylation (p = 0.026).
24254641	5	69	from	decrease	868:875	arg1	structures					893:902	triantennary structures	880:902	triantennary structures (p = 0.047)	880:914	N-glycans of PCa compared to BPH were characterized by a significant decrease in triantennary structures (p = 0.047) and overall fucosylation (p = 0.026).
24254641	2	70	theme	potential	331:339	arg1	profiles					317:324	prostatic protein glycosylation profiles	285:324	prostatic protein glycosylation profiles	285:324	Therefore, we investigated prostatic protein glycosylation profiles as a potential biomarker.
24254641	2	70	theme	potential	331:339	arg1	biomarker					341:349	a potential biomarker	329:349	a potential biomarker	329:349	Therefore, we investigated prostatic protein glycosylation profiles as a potential biomarker.
24254641	4	71	theme	prostatic	712:720	arg1	proteins					722:729	prostatic proteins	712:729	prostatic proteins	712:729	Through squeezing of the prostate, a sufficient amount of prostatic proteins was obtained for direct structural analyses of N-glycan structures.
24254641	6	72	theme	glycoprofile	1002:1013	arg1	marker					1015:1020	the urinary glycoprofile marker	990:1020	the urinary glycoprofile marker	990:1020	Prostate-specific antigen (PSA) and the urinary glycoprofile marker showed comparable overall receiver operating characteristic curve analysis as well as in the diagnostic gray zone with serum PSA values between 4 and 10 μg/L. However, when combining PSA and the urinary glycoprofile marker, the latter gave an additive diagnostic value to serum PSA (p ≤ 0.001).
24254641	3	73	theme	=	568:568	arg1	n					566:566	n = 42	566:571	n = 42	566:571	We determined the urinary asparagine-linked glycan (N-glycan) profile of prostatic proteins of healthy volunteers (n = 25), patients with benign prostate hyperplasia (BPH; n = 62) and newly diagnosed PCa patients (n = 42) using DNA-sequencer-assisted fluorophore-assisted carbohydrate electrophoresis.
24254641	3	73	theme	=	568:568	arg1	patients					556:563	newly diagnosed PCa patients	536:563	newly diagnosed PCa patients (n = 42)	536:572	We determined the urinary asparagine-linked glycan (N-glycan) profile of prostatic proteins of healthy volunteers (n = 25), patients with benign prostate hyperplasia (BPH; n = 62) and newly diagnosed PCa patients (n = 42) using DNA-sequencer-assisted fluorophore-assisted carbohydrate electrophoresis.
24254641	4	74	theme	prostate	679:686	arg1	squeezing					662:670	squeezing	662:670	squeezing of the prostate	662:686	Through squeezing of the prostate, a sufficient amount of prostatic proteins was obtained for direct structural analyses of N-glycan structures.
24254641	6	75	theme	gray	1126:1129	arg1	zone					1131:1134	the diagnostic gray zone	1111:1134	the diagnostic gray zone with serum PSA values between 4 and 10 μg/L.	1111:1179	Prostate-specific antigen (PSA) and the urinary glycoprofile marker showed comparable overall receiver operating characteristic curve analysis as well as in the diagnostic gray zone with serum PSA values between 4 and 10 μg/L. However, when combining PSA and the urinary glycoprofile marker, the latter gave an additive diagnostic value to serum PSA (p ≤ 0.001).
24254641	6	76	theme	urinary	994:1000	arg1	marker					1015:1020	the urinary glycoprofile marker	990:1020	the urinary glycoprofile marker	990:1020	Prostate-specific antigen (PSA) and the urinary glycoprofile marker showed comparable overall receiver operating characteristic curve analysis as well as in the diagnostic gray zone with serum PSA values between 4 and 10 μg/L. However, when combining PSA and the urinary glycoprofile marker, the latter gave an additive diagnostic value to serum PSA (p ≤ 0.001).
24254641	7	77	theme	N-glycosylation	1332:1346	arg1	profiling					1348:1356	N-glycosylation profiling	1332:1356	N-glycosylation profiling	1332:1356	In conclusion, N-glycosylation profiling demonstrated differences between BPH and PCa.
24254641	3	78	theme	asparagine-linked	378:394	arg1	profile					414:420	the urinary asparagine-linked glycan (N-glycan) profile	366:420	the urinary asparagine-linked glycan (N-glycan) profile of prostatic proteins of healthy volunteers (n = 25), patients with benign prostate hyperplasia (BPH; n = 62) and newly diagnosed PCa patients (n = 42)	366:572	We determined the urinary asparagine-linked glycan (N-glycan) profile of prostatic proteins of healthy volunteers (n = 25), patients with benign prostate hyperplasia (BPH; n = 62) and newly diagnosed PCa patients (n = 42) using DNA-sequencer-assisted fluorophore-assisted carbohydrate electrophoresis.
24407671	3	0	theme	organic	328:334	arg1	polysaccharides					336:350	organic polysaccharides	328:350	organic polysaccharides	328:350	This work aims at investigating the effect of organic polysaccharides, namely carboxymethylcellulose (CMC) or chitosan (Ch) differing by their side groups, on the resulting structure of montmorillonite-based nanocomposites.
24407671	3	0	theme	organic	328:334	arg1	carboxymethylcellulose					360:381	carboxymethylcellulose	360:381	carboxymethylcellulose (CMC)	360:387	This work aims at investigating the effect of organic polysaccharides, namely carboxymethylcellulose (CMC) or chitosan (Ch) differing by their side groups, on the resulting structure of montmorillonite-based nanocomposites.
24407671	3	0	theme	organic	328:334	arg1	chitosan					392:399	chitosan	392:399	chitosan	392:399	This work aims at investigating the effect of organic polysaccharides, namely carboxymethylcellulose (CMC) or chitosan (Ch) differing by their side groups, on the resulting structure of montmorillonite-based nanocomposites.
24407671	7	1	theme	surface	1323:1329	arg1	adsorption					1331:1340	surface adsorption	1323:1340	surface adsorption	1323:1340	In this case, two mechanisms are in competition: surface adsorption and intercalation between exfoliated platelets.
24407671	7	1	theme	surface	1323:1329	arg1	mechanisms					1292:1301	two mechanisms	1288:1301	two mechanisms	1288:1301	In this case, two mechanisms are in competition: surface adsorption and intercalation between exfoliated platelets.
24407671	4	2	theme	size	760:763	arg1	analysis					765:772	particle size analysis	751:772	particle size analysis	751:772	EXPERIMENTS The effect of sodium hexametaphosphate and of two polysaccharide derivatives (carboxymethylcellulose and chitosan) combined with montmorillonite on the structure and microstructure of resulting composite films was investigated using particle size analysis, rheological measurements, thermogravimetric analysis, X-ray diffraction, scanning electron microscopy and flexural properties measurements of the textured films.
24407671	6	3	theme	montmorillonite/sodium	1083:1104	arg1	films					1124:1128	the montmorillonite/sodium hexametaphosphate films	1079:1128	the montmorillonite/sodium hexametaphosphate films	1079:1128	The high organization (and resulting toughness) of the montmorillonite/sodium hexametaphosphate films results from an exfoliated then layered microstructure, whereas the addition of polysaccharide derivatives leads to the particle agglomeration.
24407671	3	4	theme	polysaccharides	336:350	arg1	effect					318:323	the effect	314:323	the effect of organic polysaccharides, namely carboxymethylcellulose (CMC) or chitosan (Ch) differing by their side groups, on the resulting structure of montmorillonite-based nanocomposites	314:503	This work aims at investigating the effect of organic polysaccharides, namely carboxymethylcellulose (CMC) or chitosan (Ch) differing by their side groups, on the resulting structure of montmorillonite-based nanocomposites.
24407671	4	5	theme	sodium	532:537	arg1	hexametaphosphate					539:555	sodium hexametaphosphate	532:555	sodium hexametaphosphate	532:555	EXPERIMENTS The effect of sodium hexametaphosphate and of two polysaccharide derivatives (carboxymethylcellulose and chitosan) combined with montmorillonite on the structure and microstructure of resulting composite films was investigated using particle size analysis, rheological measurements, thermogravimetric analysis, X-ray diffraction, scanning electron microscopy and flexural properties measurements of the textured films.
24407671	6	6	dep	toughness	1065:1073	arg1	resulting					1055:1063	resulting	1055:1063	resulting	1055:1063	The high organization (and resulting toughness) of the montmorillonite/sodium hexametaphosphate films results from an exfoliated then layered microstructure, whereas the addition of polysaccharide derivatives leads to the particle agglomeration.
24407671	4	7	theme	particle	751:758	arg1	analysis					765:772	particle size analysis	751:772	particle size analysis	751:772	EXPERIMENTS The effect of sodium hexametaphosphate and of two polysaccharide derivatives (carboxymethylcellulose and chitosan) combined with montmorillonite on the structure and microstructure of resulting composite films was investigated using particle size analysis, rheological measurements, thermogravimetric analysis, X-ray diffraction, scanning electron microscopy and flexural properties measurements of the textured films.
24407671	4	8	from	effect	522:527	arg1	microstructure					684:697	microstructure	684:697	microstructure	684:697	EXPERIMENTS The effect of sodium hexametaphosphate and of two polysaccharide derivatives (carboxymethylcellulose and chitosan) combined with montmorillonite on the structure and microstructure of resulting composite films was investigated using particle size analysis, rheological measurements, thermogravimetric analysis, X-ray diffraction, scanning electron microscopy and flexural properties measurements of the textured films.
24407671	4	8	from	effect	522:527	arg1	structure					670:678	structure	670:678	structure	670:678	EXPERIMENTS The effect of sodium hexametaphosphate and of two polysaccharide derivatives (carboxymethylcellulose and chitosan) combined with montmorillonite on the structure and microstructure of resulting composite films was investigated using particle size analysis, rheological measurements, thermogravimetric analysis, X-ray diffraction, scanning electron microscopy and flexural properties measurements of the textured films.
24407671	4	9	dep	analysis	765:772	arg1	measurements					901:912	measurements	901:912	measurements	901:912	EXPERIMENTS The effect of sodium hexametaphosphate and of two polysaccharide derivatives (carboxymethylcellulose and chitosan) combined with montmorillonite on the structure and microstructure of resulting composite films was investigated using particle size analysis, rheological measurements, thermogravimetric analysis, X-ray diffraction, scanning electron microscopy and flexural properties measurements of the textured films.
24407671	4	10	theme	X-ray	829:833	arg1	diffraction					835:845	X-ray diffraction	829:845	X-ray diffraction	829:845	EXPERIMENTS The effect of sodium hexametaphosphate and of two polysaccharide derivatives (carboxymethylcellulose and chitosan) combined with montmorillonite on the structure and microstructure of resulting composite films was investigated using particle size analysis, rheological measurements, thermogravimetric analysis, X-ray diffraction, scanning electron microscopy and flexural properties measurements of the textured films.
24407671	7	11	theme	exfoliated	1368:1377	arg1	platelets					1379:1387	exfoliated platelets	1368:1387	exfoliated platelets	1368:1387	In this case, two mechanisms are in competition: surface adsorption and intercalation between exfoliated platelets.
24407671	1	12	theme	HYPOTHESES	91:100	arg1	Montmorillonite					102:116	HYPOTHESES Montmorillonite	91:116	HYPOTHESES Montmorillonite	91:116	HYPOTHESES Montmorillonite, an abundant raw material, is a good candidate to obtain textured nanocomposites.
24407671	1	12	theme	HYPOTHESES	91:100	arg1	candidate					155:163	a good candidate	148:163	a good candidate to obtain textured nanocomposites	148:197	HYPOTHESES Montmorillonite, an abundant raw material, is a good candidate to obtain textured nanocomposites.
24407671	1	12	theme	HYPOTHESES	91:100	arg1	material					135:142	an abundant raw material	119:142	an abundant raw material	119:142	HYPOTHESES Montmorillonite, an abundant raw material, is a good candidate to obtain textured nanocomposites.
24407671	3	13	theme	nanocomposites	490:503	arg1	structure					455:463	the resulting structure	441:463	the resulting structure of montmorillonite-based nanocomposites	441:503	This work aims at investigating the effect of organic polysaccharides, namely carboxymethylcellulose (CMC) or chitosan (Ch) differing by their side groups, on the resulting structure of montmorillonite-based nanocomposites.
24407671	4	14	theme	films	930:934	arg1	measurements					787:798	rheological measurements	775:798	rheological measurements	775:798	EXPERIMENTS The effect of sodium hexametaphosphate and of two polysaccharide derivatives (carboxymethylcellulose and chitosan) combined with montmorillonite on the structure and microstructure of resulting composite films was investigated using particle size analysis, rheological measurements, thermogravimetric analysis, X-ray diffraction, scanning electron microscopy and flexural properties measurements of the textured films.
24407671	4	14	theme	films	930:934	arg1	diffraction					835:845	X-ray diffraction	829:845	X-ray diffraction	829:845	EXPERIMENTS The effect of sodium hexametaphosphate and of two polysaccharide derivatives (carboxymethylcellulose and chitosan) combined with montmorillonite on the structure and microstructure of resulting composite films was investigated using particle size analysis, rheological measurements, thermogravimetric analysis, X-ray diffraction, scanning electron microscopy and flexural properties measurements of the textured films.
24407671	4	14	theme	films	930:934	arg1	properties					890:899	flexural properties	881:899	flexural properties	881:899	EXPERIMENTS The effect of sodium hexametaphosphate and of two polysaccharide derivatives (carboxymethylcellulose and chitosan) combined with montmorillonite on the structure and microstructure of resulting composite films was investigated using particle size analysis, rheological measurements, thermogravimetric analysis, X-ray diffraction, scanning electron microscopy and flexural properties measurements of the textured films.
24407671	4	14	theme	films	930:934	arg1	analysis					819:826	thermogravimetric analysis	801:826	thermogravimetric analysis	801:826	EXPERIMENTS The effect of sodium hexametaphosphate and of two polysaccharide derivatives (carboxymethylcellulose and chitosan) combined with montmorillonite on the structure and microstructure of resulting composite films was investigated using particle size analysis, rheological measurements, thermogravimetric analysis, X-ray diffraction, scanning electron microscopy and flexural properties measurements of the textured films.
24407671	4	14	theme	films	930:934	arg1	microscopy					866:875	scanning electron microscopy	848:875	scanning electron microscopy	848:875	EXPERIMENTS The effect of sodium hexametaphosphate and of two polysaccharide derivatives (carboxymethylcellulose and chitosan) combined with montmorillonite on the structure and microstructure of resulting composite films was investigated using particle size analysis, rheological measurements, thermogravimetric analysis, X-ray diffraction, scanning electron microscopy and flexural properties measurements of the textured films.
24407671	4	14	theme	films	930:934	arg1	analysis					765:772	particle size analysis	751:772	particle size analysis	751:772	EXPERIMENTS The effect of sodium hexametaphosphate and of two polysaccharide derivatives (carboxymethylcellulose and chitosan) combined with montmorillonite on the structure and microstructure of resulting composite films was investigated using particle size analysis, rheological measurements, thermogravimetric analysis, X-ray diffraction, scanning electron microscopy and flexural properties measurements of the textured films.
24407671	4	15	theme	scanning	848:855	arg1	microscopy					866:875	scanning electron microscopy	848:875	scanning electron microscopy	848:875	EXPERIMENTS The effect of sodium hexametaphosphate and of two polysaccharide derivatives (carboxymethylcellulose and chitosan) combined with montmorillonite on the structure and microstructure of resulting composite films was investigated using particle size analysis, rheological measurements, thermogravimetric analysis, X-ray diffraction, scanning electron microscopy and flexural properties measurements of the textured films.
24407671	4	16	theme	composite	712:720	arg1	films					722:726	resulting composite films	702:726	resulting composite films	702:726	EXPERIMENTS The effect of sodium hexametaphosphate and of two polysaccharide derivatives (carboxymethylcellulose and chitosan) combined with montmorillonite on the structure and microstructure of resulting composite films was investigated using particle size analysis, rheological measurements, thermogravimetric analysis, X-ray diffraction, scanning electron microscopy and flexural properties measurements of the textured films.
24407671	4	17	dep	derivatives	583:593	arg1	derivatives					583:593	two polysaccharide derivatives	564:593	two polysaccharide derivatives (carboxymethylcellulose and chitosan) combined with montmorillonite	564:661	EXPERIMENTS The effect of sodium hexametaphosphate and of two polysaccharide derivatives (carboxymethylcellulose and chitosan) combined with montmorillonite on the structure and microstructure of resulting composite films was investigated using particle size analysis, rheological measurements, thermogravimetric analysis, X-ray diffraction, scanning electron microscopy and flexural properties measurements of the textured films.
24407671	4	17	dep	derivatives	583:593	arg1	chitosan					623:630	chitosan	623:630	chitosan	623:630	EXPERIMENTS The effect of sodium hexametaphosphate and of two polysaccharide derivatives (carboxymethylcellulose and chitosan) combined with montmorillonite on the structure and microstructure of resulting composite films was investigated using particle size analysis, rheological measurements, thermogravimetric analysis, X-ray diffraction, scanning electron microscopy and flexural properties measurements of the textured films.
24407671	4	17	dep	derivatives	583:593	arg1	carboxymethylcellulose					596:617	carboxymethylcellulose	596:617	carboxymethylcellulose	596:617	EXPERIMENTS The effect of sodium hexametaphosphate and of two polysaccharide derivatives (carboxymethylcellulose and chitosan) combined with montmorillonite on the structure and microstructure of resulting composite films was investigated using particle size analysis, rheological measurements, thermogravimetric analysis, X-ray diffraction, scanning electron microscopy and flexural properties measurements of the textured films.
24407671	4	18	theme	textured	921:928	arg1	films					930:934	the textured films	917:934	the textured films	917:934	EXPERIMENTS The effect of sodium hexametaphosphate and of two polysaccharide derivatives (carboxymethylcellulose and chitosan) combined with montmorillonite on the structure and microstructure of resulting composite films was investigated using particle size analysis, rheological measurements, thermogravimetric analysis, X-ray diffraction, scanning electron microscopy and flexural properties measurements of the textured films.
24407671	4	19	theme	resulting	702:710	arg1	films					722:726	resulting composite films	702:726	resulting composite films	702:726	EXPERIMENTS The effect of sodium hexametaphosphate and of two polysaccharide derivatives (carboxymethylcellulose and chitosan) combined with montmorillonite on the structure and microstructure of resulting composite films was investigated using particle size analysis, rheological measurements, thermogravimetric analysis, X-ray diffraction, scanning electron microscopy and flexural properties measurements of the textured films.
24407671	4	20	dep	EXPERIMENTS	506:516	arg1	investigated					732:743	investigated	732:743	was investigated using particle size analysis, rheological measurements, thermogravimetric analysis, X-ray diffraction, scanning electron microscopy and flexural properties measurements of the textured films	728:934	EXPERIMENTS The effect of sodium hexametaphosphate and of two polysaccharide derivatives (carboxymethylcellulose and chitosan) combined with montmorillonite on the structure and microstructure of resulting composite films was investigated using particle size analysis, rheological measurements, thermogravimetric analysis, X-ray diffraction, scanning electron microscopy and flexural properties measurements of the textured films.
24407671	4	21	theme	thermogravimetric	801:817	arg1	analysis					819:826	thermogravimetric analysis	801:826	thermogravimetric analysis	801:826	EXPERIMENTS The effect of sodium hexametaphosphate and of two polysaccharide derivatives (carboxymethylcellulose and chitosan) combined with montmorillonite on the structure and microstructure of resulting composite films was investigated using particle size analysis, rheological measurements, thermogravimetric analysis, X-ray diffraction, scanning electron microscopy and flexural properties measurements of the textured films.
24407671	3	22	theme	side	425:428	arg1	groups					430:435	their side groups	419:435	their side groups	419:435	This work aims at investigating the effect of organic polysaccharides, namely carboxymethylcellulose (CMC) or chitosan (Ch) differing by their side groups, on the resulting structure of montmorillonite-based nanocomposites.
24407671	0	23	theme	Structural	0:9	arg1	studies					31:37	Structural and microstructural studies	0:37	Structural and microstructural studies of montmorillonite-based multilayer nanocomposites	0:88	Structural and microstructural studies of montmorillonite-based multilayer nanocomposites.
24407671	4	24	theme	derivatives	583:593	arg1	effect					522:527	The effect	518:527	The effect of sodium hexametaphosphate and of two polysaccharide derivatives (carboxymethylcellulose and chitosan) combined with montmorillonite on the structure and microstructure of resulting composite films	518:726	EXPERIMENTS The effect of sodium hexametaphosphate and of two polysaccharide derivatives (carboxymethylcellulose and chitosan) combined with montmorillonite on the structure and microstructure of resulting composite films was investigated using particle size analysis, rheological measurements, thermogravimetric analysis, X-ray diffraction, scanning electron microscopy and flexural properties measurements of the textured films.
24407671	4	25	theme	rheological	775:785	arg1	measurements					787:798	rheological measurements	775:798	rheological measurements	775:798	EXPERIMENTS The effect of sodium hexametaphosphate and of two polysaccharide derivatives (carboxymethylcellulose and chitosan) combined with montmorillonite on the structure and microstructure of resulting composite films was investigated using particle size analysis, rheological measurements, thermogravimetric analysis, X-ray diffraction, scanning electron microscopy and flexural properties measurements of the textured films.
24407671	1	26	theme	abundant	122:129	arg1	Montmorillonite					102:116	HYPOTHESES Montmorillonite	91:116	HYPOTHESES Montmorillonite	91:116	HYPOTHESES Montmorillonite, an abundant raw material, is a good candidate to obtain textured nanocomposites.
24407671	1	26	theme	abundant	122:129	arg1	material					135:142	an abundant raw material	119:142	an abundant raw material	119:142	HYPOTHESES Montmorillonite, an abundant raw material, is a good candidate to obtain textured nanocomposites.
24407671	4	27	theme	films	722:726	arg1	microstructure					684:697	microstructure	684:697	microstructure	684:697	EXPERIMENTS The effect of sodium hexametaphosphate and of two polysaccharide derivatives (carboxymethylcellulose and chitosan) combined with montmorillonite on the structure and microstructure of resulting composite films was investigated using particle size analysis, rheological measurements, thermogravimetric analysis, X-ray diffraction, scanning electron microscopy and flexural properties measurements of the textured films.
24407671	4	27	theme	films	722:726	arg1	structure					670:678	structure	670:678	structure	670:678	EXPERIMENTS The effect of sodium hexametaphosphate and of two polysaccharide derivatives (carboxymethylcellulose and chitosan) combined with montmorillonite on the structure and microstructure of resulting composite films was investigated using particle size analysis, rheological measurements, thermogravimetric analysis, X-ray diffraction, scanning electron microscopy and flexural properties measurements of the textured films.
24407671	4	28	theme	polysaccharide	568:581	arg1	derivatives					583:593	two polysaccharide derivatives	564:593	two polysaccharide derivatives (carboxymethylcellulose and chitosan) combined with montmorillonite	564:661	EXPERIMENTS The effect of sodium hexametaphosphate and of two polysaccharide derivatives (carboxymethylcellulose and chitosan) combined with montmorillonite on the structure and microstructure of resulting composite films was investigated using particle size analysis, rheological measurements, thermogravimetric analysis, X-ray diffraction, scanning electron microscopy and flexural properties measurements of the textured films.
24407671	4	28	theme	polysaccharide	568:581	arg1	chitosan					623:630	chitosan	623:630	chitosan	623:630	EXPERIMENTS The effect of sodium hexametaphosphate and of two polysaccharide derivatives (carboxymethylcellulose and chitosan) combined with montmorillonite on the structure and microstructure of resulting composite films was investigated using particle size analysis, rheological measurements, thermogravimetric analysis, X-ray diffraction, scanning electron microscopy and flexural properties measurements of the textured films.
24407671	4	28	theme	polysaccharide	568:581	arg1	carboxymethylcellulose					596:617	carboxymethylcellulose	596:617	carboxymethylcellulose	596:617	EXPERIMENTS The effect of sodium hexametaphosphate and of two polysaccharide derivatives (carboxymethylcellulose and chitosan) combined with montmorillonite on the structure and microstructure of resulting composite films was investigated using particle size analysis, rheological measurements, thermogravimetric analysis, X-ray diffraction, scanning electron microscopy and flexural properties measurements of the textured films.
24407671	1	29	theme	raw	131:133	arg1	Montmorillonite					102:116	HYPOTHESES Montmorillonite	91:116	HYPOTHESES Montmorillonite	91:116	HYPOTHESES Montmorillonite, an abundant raw material, is a good candidate to obtain textured nanocomposites.
24407671	1	29	theme	raw	131:133	arg1	material					135:142	an abundant raw material	119:142	an abundant raw material	119:142	HYPOTHESES Montmorillonite, an abundant raw material, is a good candidate to obtain textured nanocomposites.
24407671	6	30	theme	layered	1162:1168	arg1	microstructure					1170:1183	an exfoliated then layered microstructure	1143:1183	an exfoliated then layered microstructure	1143:1183	The high organization (and resulting toughness) of the montmorillonite/sodium hexametaphosphate films results from an exfoliated then layered microstructure, whereas the addition of polysaccharide derivatives leads to the particle agglomeration.
24407671	0	31	theme	microstructural	15:29	arg1	studies					31:37	Structural and microstructural studies	0:37	Structural and microstructural studies of montmorillonite-based multilayer nanocomposites	0:88	Structural and microstructural studies of montmorillonite-based multilayer nanocomposites.
24407671	7	32	from	intercalation	1346:1358	arg1	competition					1310:1320	competition	1310:1320	competition	1310:1320	In this case, two mechanisms are in competition: surface adsorption and intercalation between exfoliated platelets.
24407671	7	32	from	intercalation	1346:1358	arg1	case					1282:1285	this case	1277:1285	this case	1277:1285	In this case, two mechanisms are in competition: surface adsorption and intercalation between exfoliated platelets.
24407671	1	33	theme	textured	175:182	arg1	nanocomposites					184:197	textured nanocomposites	175:197	textured nanocomposites	175:197	HYPOTHESES Montmorillonite, an abundant raw material, is a good candidate to obtain textured nanocomposites.
24407671	6	34	theme	derivatives	1225:1235	arg1	addition					1198:1205	the addition	1194:1205	the addition of polysaccharide derivatives	1194:1235	The high organization (and resulting toughness) of the montmorillonite/sodium hexametaphosphate films results from an exfoliated then layered microstructure, whereas the addition of polysaccharide derivatives leads to the particle agglomeration.
24407671	0	35	theme	montmorillonite-based	42:62	arg1	nanocomposites					75:88	montmorillonite-based multilayer nanocomposites	42:88	montmorillonite-based multilayer nanocomposites	42:88	Structural and microstructural studies of montmorillonite-based multilayer nanocomposites.
24407671	6	36	theme	exfoliated	1146:1155	arg1	microstructure					1170:1183	an exfoliated then layered microstructure	1143:1183	an exfoliated then layered microstructure	1143:1183	The high organization (and resulting toughness) of the montmorillonite/sodium hexametaphosphate films results from an exfoliated then layered microstructure, whereas the addition of polysaccharide derivatives leads to the particle agglomeration.
24407671	6	37	theme	films	1124:1128	arg1	organization					1037:1048	The high organization	1028:1048	The high organization (and resulting toughness) of the montmorillonite/sodium hexametaphosphate films	1028:1128	The high organization (and resulting toughness) of the montmorillonite/sodium hexametaphosphate films results from an exfoliated then layered microstructure, whereas the addition of polysaccharide derivatives leads to the particle agglomeration.
24407671	6	37	theme	films	1124:1128	arg1	toughness					1065:1073	and resulting toughness	1051:1073	toughness	1065:1073	The high organization (and resulting toughness) of the montmorillonite/sodium hexametaphosphate films results from an exfoliated then layered microstructure, whereas the addition of polysaccharide derivatives leads to the particle agglomeration.
24407671	3	38	theme	resulting	445:453	arg1	structure					455:463	the resulting structure	441:463	the resulting structure of montmorillonite-based nanocomposites	441:503	This work aims at investigating the effect of organic polysaccharides, namely carboxymethylcellulose (CMC) or chitosan (Ch) differing by their side groups, on the resulting structure of montmorillonite-based nanocomposites.
24407671	0	39	theme	nanocomposites	75:88	arg1	studies					31:37	Structural and microstructural studies	0:37	Structural and microstructural studies of montmorillonite-based multilayer nanocomposites	0:88	Structural and microstructural studies of montmorillonite-based multilayer nanocomposites.
24407671	4	40	theme	hexametaphosphate	539:555	arg1	effect					522:527	The effect	518:527	The effect of sodium hexametaphosphate and of two polysaccharide derivatives (carboxymethylcellulose and chitosan) combined with montmorillonite on the structure and microstructure of resulting composite films	518:726	EXPERIMENTS The effect of sodium hexametaphosphate and of two polysaccharide derivatives (carboxymethylcellulose and chitosan) combined with montmorillonite on the structure and microstructure of resulting composite films was investigated using particle size analysis, rheological measurements, thermogravimetric analysis, X-ray diffraction, scanning electron microscopy and flexural properties measurements of the textured films.
24407671	2	41	theme	composite	240:248	arg1	structure					223:231	the resulting structure	209:231	the resulting structure of the composite	209:248	However, the resulting structure of the composite depends on the dispersant used.
24407671	6	42	theme	particle	1250:1257	arg1	agglomeration					1259:1271	the particle agglomeration	1246:1271	the particle agglomeration	1246:1271	The high organization (and resulting toughness) of the montmorillonite/sodium hexametaphosphate films results from an exfoliated then layered microstructure, whereas the addition of polysaccharide derivatives leads to the particle agglomeration.
24407671	0	43	theme	multilayer	64:73	arg1	nanocomposites					75:88	montmorillonite-based multilayer nanocomposites	42:88	montmorillonite-based multilayer nanocomposites	42:88	Structural and microstructural studies of montmorillonite-based multilayer nanocomposites.
24407671	4	44	dep	structure	670:678	arg1	the					666:668	the	666:668	the	666:668	EXPERIMENTS The effect of sodium hexametaphosphate and of two polysaccharide derivatives (carboxymethylcellulose and chitosan) combined with montmorillonite on the structure and microstructure of resulting composite films was investigated using particle size analysis, rheological measurements, thermogravimetric analysis, X-ray diffraction, scanning electron microscopy and flexural properties measurements of the textured films.
24407671	7	45	from	adsorption	1331:1340	arg1	competition					1310:1320	competition	1310:1320	competition	1310:1320	In this case, two mechanisms are in competition: surface adsorption and intercalation between exfoliated platelets.
24407671	7	45	from	adsorption	1331:1340	arg1	case					1282:1285	this case	1277:1285	this case	1277:1285	In this case, two mechanisms are in competition: surface adsorption and intercalation between exfoliated platelets.
24407671	3	46	theme	montmorillonite-based	468:488	arg1	nanocomposites					490:503	montmorillonite-based nanocomposites	468:503	montmorillonite-based nanocomposites	468:503	This work aims at investigating the effect of organic polysaccharides, namely carboxymethylcellulose (CMC) or chitosan (Ch) differing by their side groups, on the resulting structure of montmorillonite-based nanocomposites.
24407671	2	47	theme	resulting	213:221	arg1	structure					223:231	the resulting structure	209:231	the resulting structure of the composite	209:248	However, the resulting structure of the composite depends on the dispersant used.
24407671	4	48	theme	electron	857:864	arg1	microscopy					866:875	scanning electron microscopy	848:875	scanning electron microscopy	848:875	EXPERIMENTS The effect of sodium hexametaphosphate and of two polysaccharide derivatives (carboxymethylcellulose and chitosan) combined with montmorillonite on the structure and microstructure of resulting composite films was investigated using particle size analysis, rheological measurements, thermogravimetric analysis, X-ray diffraction, scanning electron microscopy and flexural properties measurements of the textured films.
24407671	1	49	theme	good	150:153	arg1	Montmorillonite					102:116	HYPOTHESES Montmorillonite	91:116	HYPOTHESES Montmorillonite	91:116	HYPOTHESES Montmorillonite, an abundant raw material, is a good candidate to obtain textured nanocomposites.
24407671	1	49	theme	good	150:153	arg1	candidate					155:163	a good candidate	148:163	a good candidate to obtain textured nanocomposites	148:197	HYPOTHESES Montmorillonite, an abundant raw material, is a good candidate to obtain textured nanocomposites.
24407671	5	50	theme	FINDINGS	937:944	arg1	Results					946:952	FINDINGS Results	937:952	FINDINGS Results	937:952	FINDINGS Results showed that the film structure and microstructure depend on the additive.
24407671	4	51	theme	flexural	881:888	arg1	properties					890:899	flexural properties	881:899	flexural properties	881:899	EXPERIMENTS The effect of sodium hexametaphosphate and of two polysaccharide derivatives (carboxymethylcellulose and chitosan) combined with montmorillonite on the structure and microstructure of resulting composite films was investigated using particle size analysis, rheological measurements, thermogravimetric analysis, X-ray diffraction, scanning electron microscopy and flexural properties measurements of the textured films.
24407671	5	52	theme	film	970:973	arg1	structure					975:983	the film structure	966:983	the film structure	966:983	FINDINGS Results showed that the film structure and microstructure depend on the additive.
24407671	3	53	from	effect	318:323	arg1	structure					455:463	the resulting structure	441:463	the resulting structure of montmorillonite-based nanocomposites	441:503	This work aims at investigating the effect of organic polysaccharides, namely carboxymethylcellulose (CMC) or chitosan (Ch) differing by their side groups, on the resulting structure of montmorillonite-based nanocomposites.
24407671	6	54	theme	polysaccharide	1210:1223	arg1	derivatives					1225:1235	polysaccharide derivatives	1210:1235	polysaccharide derivatives	1210:1235	The high organization (and resulting toughness) of the montmorillonite/sodium hexametaphosphate films results from an exfoliated then layered microstructure, whereas the addition of polysaccharide derivatives leads to the particle agglomeration.
24407671	6	55	theme	high	1032:1035	arg1	organization					1037:1048	The high organization	1028:1048	The high organization (and resulting toughness) of the montmorillonite/sodium hexametaphosphate films	1028:1128	The high organization (and resulting toughness) of the montmorillonite/sodium hexametaphosphate films results from an exfoliated then layered microstructure, whereas the addition of polysaccharide derivatives leads to the particle agglomeration.
24407671	6	55	theme	high	1032:1035	arg1	toughness					1065:1073	and resulting toughness	1051:1073	toughness	1065:1073	The high organization (and resulting toughness) of the montmorillonite/sodium hexametaphosphate films results from an exfoliated then layered microstructure, whereas the addition of polysaccharide derivatives leads to the particle agglomeration.
24407671	6	56	theme	hexametaphosphate	1106:1122	arg1	films					1124:1128	the montmorillonite/sodium hexametaphosphate films	1079:1128	the montmorillonite/sodium hexametaphosphate films	1079:1128	The high organization (and resulting toughness) of the montmorillonite/sodium hexametaphosphate films results from an exfoliated then layered microstructure, whereas the addition of polysaccharide derivatives leads to the particle agglomeration.
25659710	6	0	theme	-linked	1232:1238	arg1	α-d-Manp					1240:1247	(1→3)-linked α-d-Manp	1227:1247	(1→3)-linked α-d-Manp	1227:1247	Methylation analysis and NMR spectroscopy revealed that the backbone of poly-mannose consisting of (1→3)-linked β-d-Manp, (1→3)-linked α-d-Manp, and (1→6)-linked α-d-Manp residues, and the main chain were branched at the O-2, O-3, O-4, O-6 position.
25659710	6	1	theme	main	1294:1297	arg1	chain					1299:1303	the main chain	1290:1303	the main chain	1290:1303	Methylation analysis and NMR spectroscopy revealed that the backbone of poly-mannose consisting of (1→3)-linked β-d-Manp, (1→3)-linked α-d-Manp, and (1→6)-linked α-d-Manp residues, and the main chain were branched at the O-2, O-3, O-4, O-6 position.
25659710	1	2	theme	microwave	281:289	arg1	irradiation					291:301	microwave irradiation	281:301	microwave irradiation	281:301	Poly-mannose with molecular weight of 2.457 kDa was synthesized using d-mannose as substrate and phosphoric acid as catalyst under the condition of microwave irradiation for the first time.
25659710	5	3	theme	d-mannose	1094:1102	arg1	polymerization					1076:1089	the polymerization	1072:1089	the polymerization of d-mannose	1072:1102	HPAEC analysis showed that the composition of synthetic polysaccharides was d-mannose, its purity was demonstrated by HPGPC as a single symmetrical sharp peak, and additionally IR spectra demonstrated the polymerization of d-mannose.
25659710	6	4	theme	O-6	1341:1343	arg1	position					1345:1352	O-6 position	1341:1352	O-6 position	1341:1352	Methylation analysis and NMR spectroscopy revealed that the backbone of poly-mannose consisting of (1→3)-linked β-d-Manp, (1→3)-linked α-d-Manp, and (1→6)-linked α-d-Manp residues, and the main chain were branched at the O-2, O-3, O-4, O-6 position.
25659710	6	4	theme	O-6	1341:1343	arg1	O-3					1331:1333	O-3	1331:1333	O-3	1331:1333	Methylation analysis and NMR spectroscopy revealed that the backbone of poly-mannose consisting of (1→3)-linked β-d-Manp, (1→3)-linked α-d-Manp, and (1→6)-linked α-d-Manp residues, and the main chain were branched at the O-2, O-3, O-4, O-6 position.
25659710	1	5	theme	irradiation	291:301	arg1	condition					268:276	the condition	264:276	the condition of microwave irradiation for the first time	264:320	Poly-mannose with molecular weight of 2.457 kDa was synthesized using d-mannose as substrate and phosphoric acid as catalyst under the condition of microwave irradiation for the first time.
25659710	0	6	theme	microwave	110:118	arg1	irradiation					120:130	microwave irradiation	110:130	microwave irradiation	110:130	Preparation and structural characterization of poly-mannose synthesized by phosphoric acid catalyzation under microwave irradiation.
25659710	4	7	theme	anion-exchange	653:666	arg1	chromatography					668:681	high-performance anion-exchange chromatography	636:681	high-performance anion-exchange chromatography (HPAEC)	636:689	After purified by Sepherdex G-25 column chromatography, the structural features of poly-mannose were investigated by high-performance anion-exchange chromatography (HPAEC), high-performance gel-permeation chromatography (HPGPC), infrared (IR) spectroscopy, methylation analysis and NMR spectroscopy analysis ((1)H, (13)C, COSY, TOCSY, HMQC, and HMBC).
25659710	4	7	theme	anion-exchange	653:666	arg1	analysis					788:795	methylation analysis	776:795	methylation analysis	776:795	After purified by Sepherdex G-25 column chromatography, the structural features of poly-mannose were investigated by high-performance anion-exchange chromatography (HPAEC), high-performance gel-permeation chromatography (HPGPC), infrared (IR) spectroscopy, methylation analysis and NMR spectroscopy analysis ((1)H, (13)C, COSY, TOCSY, HMQC, and HMBC).
25659710	4	7	theme	anion-exchange	653:666	arg1	chromatography					724:737	high-performance gel-permeation chromatography	692:737	high-performance gel-permeation chromatography (HPGPC)	692:745	After purified by Sepherdex G-25 column chromatography, the structural features of poly-mannose were investigated by high-performance anion-exchange chromatography (HPAEC), high-performance gel-permeation chromatography (HPGPC), infrared (IR) spectroscopy, methylation analysis and NMR spectroscopy analysis ((1)H, (13)C, COSY, TOCSY, HMQC, and HMBC).
25659710	4	7	theme	anion-exchange	653:666	arg1	HPAEC					684:688	HPAEC	684:688	HPAEC	684:688	After purified by Sepherdex G-25 column chromatography, the structural features of poly-mannose were investigated by high-performance anion-exchange chromatography (HPAEC), high-performance gel-permeation chromatography (HPGPC), infrared (IR) spectroscopy, methylation analysis and NMR spectroscopy analysis ((1)H, (13)C, COSY, TOCSY, HMQC, and HMBC).
25659710	4	7	theme	anion-exchange	653:666	arg1	spectroscopy					762:773	infrared (IR) spectroscopy	748:773	infrared (IR) spectroscopy	748:773	After purified by Sepherdex G-25 column chromatography, the structural features of poly-mannose were investigated by high-performance anion-exchange chromatography (HPAEC), high-performance gel-permeation chromatography (HPGPC), infrared (IR) spectroscopy, methylation analysis and NMR spectroscopy analysis ((1)H, (13)C, COSY, TOCSY, HMQC, and HMBC).
25659710	4	7	theme	anion-exchange	653:666	arg1	analysis					818:825	NMR spectroscopy analysis	801:825	NMR spectroscopy analysis	801:825	After purified by Sepherdex G-25 column chromatography, the structural features of poly-mannose were investigated by high-performance anion-exchange chromatography (HPAEC), high-performance gel-permeation chromatography (HPGPC), infrared (IR) spectroscopy, methylation analysis and NMR spectroscopy analysis ((1)H, (13)C, COSY, TOCSY, HMQC, and HMBC).
25659710	5	8	theme	synthetic	917:925	arg1	polysaccharides					927:941	synthetic polysaccharides	917:941	synthetic polysaccharides	917:941	HPAEC analysis showed that the composition of synthetic polysaccharides was d-mannose, its purity was demonstrated by HPGPC as a single symmetrical sharp peak, and additionally IR spectra demonstrated the polymerization of d-mannose.
25659710	4	9	theme	IR	758:759	arg1	spectroscopy					762:773	infrared (IR) spectroscopy	748:773	infrared (IR) spectroscopy	748:773	After purified by Sepherdex G-25 column chromatography, the structural features of poly-mannose were investigated by high-performance anion-exchange chromatography (HPAEC), high-performance gel-permeation chromatography (HPGPC), infrared (IR) spectroscopy, methylation analysis and NMR spectroscopy analysis ((1)H, (13)C, COSY, TOCSY, HMQC, and HMBC).
25659710	4	9	theme	IR	758:759	arg1	chromatography					668:681	high-performance anion-exchange chromatography	636:681	high-performance anion-exchange chromatography (HPAEC)	636:689	After purified by Sepherdex G-25 column chromatography, the structural features of poly-mannose were investigated by high-performance anion-exchange chromatography (HPAEC), high-performance gel-permeation chromatography (HPGPC), infrared (IR) spectroscopy, methylation analysis and NMR spectroscopy analysis ((1)H, (13)C, COSY, TOCSY, HMQC, and HMBC).
25659710	3	10	theme	maximum	493:499	arg1	yield					501:505	The actual maximum yield	482:505	The actual maximum yield	482:505	The actual maximum yield was 91.46%.
25659710	3	10	theme	maximum	493:499	arg1	%					516:516	91.46%	511:516	91.46%	511:516	The actual maximum yield was 91.46%.
25659710	5	11	theme	IR	1048:1049	arg1	spectra					1051:1057	IR spectra	1048:1057	IR spectra	1048:1057	HPAEC analysis showed that the composition of synthetic polysaccharides was d-mannose, its purity was demonstrated by HPGPC as a single symmetrical sharp peak, and additionally IR spectra demonstrated the polymerization of d-mannose.
25659710	1	12	theme	phosphoric	230:239	arg1	acid					241:244	phosphoric acid	230:244	phosphoric acid	230:244	Poly-mannose with molecular weight of 2.457 kDa was synthesized using d-mannose as substrate and phosphoric acid as catalyst under the condition of microwave irradiation for the first time.
25659710	4	13	theme	spectroscopy	805:816	arg1	analysis					818:825	NMR spectroscopy analysis	801:825	NMR spectroscopy analysis	801:825	After purified by Sepherdex G-25 column chromatography, the structural features of poly-mannose were investigated by high-performance anion-exchange chromatography (HPAEC), high-performance gel-permeation chromatography (HPGPC), infrared (IR) spectroscopy, methylation analysis and NMR spectroscopy analysis ((1)H, (13)C, COSY, TOCSY, HMQC, and HMBC).
25659710	4	13	theme	spectroscopy	805:816	arg1	chromatography					668:681	high-performance anion-exchange chromatography	636:681	high-performance anion-exchange chromatography (HPAEC)	636:689	After purified by Sepherdex G-25 column chromatography, the structural features of poly-mannose were investigated by high-performance anion-exchange chromatography (HPAEC), high-performance gel-permeation chromatography (HPGPC), infrared (IR) spectroscopy, methylation analysis and NMR spectroscopy analysis ((1)H, (13)C, COSY, TOCSY, HMQC, and HMBC).
25659710	5	14	theme	HPAEC	871:875	arg1	analysis					877:884	HPAEC analysis	871:884	HPAEC analysis	871:884	HPAEC analysis showed that the composition of synthetic polysaccharides was d-mannose, its purity was demonstrated by HPGPC as a single symmetrical sharp peak, and additionally IR spectra demonstrated the polymerization of d-mannose.
25659710	2	15	theme	proton	412:417	arg1	concentration					419:431	proton concentration	412:431	proton concentration 2.5 mol/L	412:441	The optimum reaction conditions were microwave output power of 900 W, temperature 115°C, proton concentration 2.5 mol/L, and microwave irradiation time 5 min.
25659710	4	16	theme	NMR	801:803	arg1	analysis					818:825	NMR spectroscopy analysis	801:825	NMR spectroscopy analysis	801:825	After purified by Sepherdex G-25 column chromatography, the structural features of poly-mannose were investigated by high-performance anion-exchange chromatography (HPAEC), high-performance gel-permeation chromatography (HPGPC), infrared (IR) spectroscopy, methylation analysis and NMR spectroscopy analysis ((1)H, (13)C, COSY, TOCSY, HMQC, and HMBC).
25659710	4	16	theme	NMR	801:803	arg1	chromatography					668:681	high-performance anion-exchange chromatography	636:681	high-performance anion-exchange chromatography (HPAEC)	636:689	After purified by Sepherdex G-25 column chromatography, the structural features of poly-mannose were investigated by high-performance anion-exchange chromatography (HPAEC), high-performance gel-permeation chromatography (HPGPC), infrared (IR) spectroscopy, methylation analysis and NMR spectroscopy analysis ((1)H, (13)C, COSY, TOCSY, HMQC, and HMBC).
25659710	6	17	link	-linked	1232:1238	arg1	α-d-Manp					1240:1247	(1→3)-linked α-d-Manp	1227:1247	(1→3)-linked α-d-Manp	1227:1247	Methylation analysis and NMR spectroscopy revealed that the backbone of poly-mannose consisting of (1→3)-linked β-d-Manp, (1→3)-linked α-d-Manp, and (1→6)-linked α-d-Manp residues, and the main chain were branched at the O-2, O-3, O-4, O-6 position.
25659710	2	18	theme	time	470:473	arg1	min					477:479	time 5 min	470:479	time 5 min	470:479	The optimum reaction conditions were microwave output power of 900 W, temperature 115°C, proton concentration 2.5 mol/L, and microwave irradiation time 5 min.
25659710	6	19	theme	-linked	1209:1215	arg1	β-d-Manp					1217:1224	(1→3)-linked β-d-Manp	1204:1224	(1→3)-linked β-d-Manp	1204:1224	Methylation analysis and NMR spectroscopy revealed that the backbone of poly-mannose consisting of (1→3)-linked β-d-Manp, (1→3)-linked α-d-Manp, and (1→6)-linked α-d-Manp residues, and the main chain were branched at the O-2, O-3, O-4, O-6 position.
25659710	4	20	theme	gel-permeation	709:722	arg1	HPGPC					740:744	HPGPC	740:744	HPGPC	740:744	After purified by Sepherdex G-25 column chromatography, the structural features of poly-mannose were investigated by high-performance anion-exchange chromatography (HPAEC), high-performance gel-permeation chromatography (HPGPC), infrared (IR) spectroscopy, methylation analysis and NMR spectroscopy analysis ((1)H, (13)C, COSY, TOCSY, HMQC, and HMBC).
25659710	4	20	theme	gel-permeation	709:722	arg1	chromatography					724:737	high-performance gel-permeation chromatography	692:737	high-performance gel-permeation chromatography (HPGPC)	692:745	After purified by Sepherdex G-25 column chromatography, the structural features of poly-mannose were investigated by high-performance anion-exchange chromatography (HPAEC), high-performance gel-permeation chromatography (HPGPC), infrared (IR) spectroscopy, methylation analysis and NMR spectroscopy analysis ((1)H, (13)C, COSY, TOCSY, HMQC, and HMBC).
25659710	4	20	theme	gel-permeation	709:722	arg1	chromatography					668:681	high-performance anion-exchange chromatography	636:681	high-performance anion-exchange chromatography (HPAEC)	636:689	After purified by Sepherdex G-25 column chromatography, the structural features of poly-mannose were investigated by high-performance anion-exchange chromatography (HPAEC), high-performance gel-permeation chromatography (HPGPC), infrared (IR) spectroscopy, methylation analysis and NMR spectroscopy analysis ((1)H, (13)C, COSY, TOCSY, HMQC, and HMBC).
25659710	1	21	with	Poly-mannose	133:144	arg1	weight					161:166	molecular weight	151:166	molecular weight of 2.457 kDa	151:179	Poly-mannose with molecular weight of 2.457 kDa was synthesized using d-mannose as substrate and phosphoric acid as catalyst under the condition of microwave irradiation for the first time.
25659710	4	22	dep	spectroscopy	762:773	arg1	H					831:831	(1)H	828:831	(1)H	828:831	After purified by Sepherdex G-25 column chromatography, the structural features of poly-mannose were investigated by high-performance anion-exchange chromatography (HPAEC), high-performance gel-permeation chromatography (HPGPC), infrared (IR) spectroscopy, methylation analysis and NMR spectroscopy analysis ((1)H, (13)C, COSY, TOCSY, HMQC, and HMBC).
25659710	2	23	theme	reaction	335:342	arg1	power					377:381	microwave output power	360:381	microwave output power of 900 W	360:390	The optimum reaction conditions were microwave output power of 900 W, temperature 115°C, proton concentration 2.5 mol/L, and microwave irradiation time 5 min.
25659710	2	23	theme	reaction	335:342	arg1	conditions					344:353	The optimum reaction conditions	323:353	The optimum reaction conditions	323:353	The optimum reaction conditions were microwave output power of 900 W, temperature 115°C, proton concentration 2.5 mol/L, and microwave irradiation time 5 min.
25659710	6	24	link	-linked	1209:1215	arg1	β-d-Manp					1217:1224	(1→3)-linked β-d-Manp	1204:1224	(1→3)-linked β-d-Manp	1204:1224	Methylation analysis and NMR spectroscopy revealed that the backbone of poly-mannose consisting of (1→3)-linked β-d-Manp, (1→3)-linked α-d-Manp, and (1→6)-linked α-d-Manp residues, and the main chain were branched at the O-2, O-3, O-4, O-6 position.
25659710	2	25	theme	temperature	393:403	arg1	115°C					405:409	temperature 115°C	393:409	temperature 115°C	393:409	The optimum reaction conditions were microwave output power of 900 W, temperature 115°C, proton concentration 2.5 mol/L, and microwave irradiation time 5 min.
25659710	2	26	theme	microwave	448:456	arg1	irradiation					458:468	microwave irradiation	448:468	microwave irradiation	448:468	The optimum reaction conditions were microwave output power of 900 W, temperature 115°C, proton concentration 2.5 mol/L, and microwave irradiation time 5 min.
25659710	1	27	theme	first	311:315	arg1	time					317:320	the first time	307:320	the first time	307:320	Poly-mannose with molecular weight of 2.457 kDa was synthesized using d-mannose as substrate and phosphoric acid as catalyst under the condition of microwave irradiation for the first time.
25659710	6	28	theme	α-d-Manp	1267:1274	arg1	residues					1276:1283	(1→6)-linked α-d-Manp residues	1254:1283	(1→6)-linked α-d-Manp residues	1254:1283	Methylation analysis and NMR spectroscopy revealed that the backbone of poly-mannose consisting of (1→3)-linked β-d-Manp, (1→3)-linked α-d-Manp, and (1→6)-linked α-d-Manp residues, and the main chain were branched at the O-2, O-3, O-4, O-6 position.
25659710	2	29	theme	optimum	327:333	arg1	power					377:381	microwave output power	360:381	microwave output power of 900 W	360:390	The optimum reaction conditions were microwave output power of 900 W, temperature 115°C, proton concentration 2.5 mol/L, and microwave irradiation time 5 min.
25659710	2	29	theme	optimum	327:333	arg1	conditions					344:353	The optimum reaction conditions	323:353	The optimum reaction conditions	323:353	The optimum reaction conditions were microwave output power of 900 W, temperature 115°C, proton concentration 2.5 mol/L, and microwave irradiation time 5 min.
25659710	6	30	dep	α-d-Manp	1240:1247	arg1	1→3					1228:1230	1→3	1228:1230	1→3	1228:1230	Methylation analysis and NMR spectroscopy revealed that the backbone of poly-mannose consisting of (1→3)-linked β-d-Manp, (1→3)-linked α-d-Manp, and (1→6)-linked α-d-Manp residues, and the main chain were branched at the O-2, O-3, O-4, O-6 position.
25659710	0	31	theme	structural	16:25	arg1	characterization					27:42	structural characterization	16:42	structural characterization	16:42	Preparation and structural characterization of poly-mannose synthesized by phosphoric acid catalyzation under microwave irradiation.
25659710	5	32	theme	polysaccharides	927:941	arg1	d-mannose					947:955	d-mannose	947:955	d-mannose	947:955	HPAEC analysis showed that the composition of synthetic polysaccharides was d-mannose, its purity was demonstrated by HPGPC as a single symmetrical sharp peak, and additionally IR spectra demonstrated the polymerization of d-mannose.
25659710	5	32	theme	polysaccharides	927:941	arg1	composition					902:912	the composition	898:912	the composition of synthetic polysaccharides	898:941	HPAEC analysis showed that the composition of synthetic polysaccharides was d-mannose, its purity was demonstrated by HPGPC as a single symmetrical sharp peak, and additionally IR spectra demonstrated the polymerization of d-mannose.
25659710	5	33	theme	sharp	1019:1023	arg1	peak					1025:1028	a single symmetrical sharp peak	998:1028	a single symmetrical sharp peak	998:1028	HPAEC analysis showed that the composition of synthetic polysaccharides was d-mannose, its purity was demonstrated by HPGPC as a single symmetrical sharp peak, and additionally IR spectra demonstrated the polymerization of d-mannose.
25659710	5	33	theme	sharp	1019:1023	arg1	purity					962:967	its purity	958:967	its purity	958:967	HPAEC analysis showed that the composition of synthetic polysaccharides was d-mannose, its purity was demonstrated by HPGPC as a single symmetrical sharp peak, and additionally IR spectra demonstrated the polymerization of d-mannose.
25659710	2	34	theme	mol/L	437:441	arg1	concentration					419:431	proton concentration	412:431	proton concentration 2.5 mol/L	412:441	The optimum reaction conditions were microwave output power of 900 W, temperature 115°C, proton concentration 2.5 mol/L, and microwave irradiation time 5 min.
25659710	0	35	theme	poly-mannose	47:58	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and structural characterization of poly-mannose synthesized by phosphoric acid catalyzation under microwave irradiation.
25659710	0	35	theme	poly-mannose	47:58	arg1	characterization					27:42	structural characterization	16:42	structural characterization	16:42	Preparation and structural characterization of poly-mannose synthesized by phosphoric acid catalyzation under microwave irradiation.
25659710	2	36	theme	output	370:375	arg1	power					377:381	microwave output power	360:381	microwave output power of 900 W	360:390	The optimum reaction conditions were microwave output power of 900 W, temperature 115°C, proton concentration 2.5 mol/L, and microwave irradiation time 5 min.
25659710	2	36	theme	output	370:375	arg1	conditions					344:353	The optimum reaction conditions	323:353	The optimum reaction conditions	323:353	The optimum reaction conditions were microwave output power of 900 W, temperature 115°C, proton concentration 2.5 mol/L, and microwave irradiation time 5 min.
25659710	4	37	dep	H	831:831	arg1	1					829:829	1	829:829	1	829:829	After purified by Sepherdex G-25 column chromatography, the structural features of poly-mannose were investigated by high-performance anion-exchange chromatography (HPAEC), high-performance gel-permeation chromatography (HPGPC), infrared (IR) spectroscopy, methylation analysis and NMR spectroscopy analysis ((1)H, (13)C, COSY, TOCSY, HMQC, and HMBC).
25659710	4	37	dep	H	831:831	arg1	C					838:838	(13)C	834:838	(13)C	834:838	After purified by Sepherdex G-25 column chromatography, the structural features of poly-mannose were investigated by high-performance anion-exchange chromatography (HPAEC), high-performance gel-permeation chromatography (HPGPC), infrared (IR) spectroscopy, methylation analysis and NMR spectroscopy analysis ((1)H, (13)C, COSY, TOCSY, HMQC, and HMBC).
25659710	4	37	dep	H	831:831	arg1	HMQC					854:857	HMQC	854:857	HMQC	854:857	After purified by Sepherdex G-25 column chromatography, the structural features of poly-mannose were investigated by high-performance anion-exchange chromatography (HPAEC), high-performance gel-permeation chromatography (HPGPC), infrared (IR) spectroscopy, methylation analysis and NMR spectroscopy analysis ((1)H, (13)C, COSY, TOCSY, HMQC, and HMBC).
25659710	4	37	dep	H	831:831	arg1	HMBC					864:867	HMBC	864:867	HMBC	864:867	After purified by Sepherdex G-25 column chromatography, the structural features of poly-mannose were investigated by high-performance anion-exchange chromatography (HPAEC), high-performance gel-permeation chromatography (HPGPC), infrared (IR) spectroscopy, methylation analysis and NMR spectroscopy analysis ((1)H, (13)C, COSY, TOCSY, HMQC, and HMBC).
25659710	4	37	dep	H	831:831	arg1	COSY					841:844	COSY	841:844	COSY	841:844	After purified by Sepherdex G-25 column chromatography, the structural features of poly-mannose were investigated by high-performance anion-exchange chromatography (HPAEC), high-performance gel-permeation chromatography (HPGPC), infrared (IR) spectroscopy, methylation analysis and NMR spectroscopy analysis ((1)H, (13)C, COSY, TOCSY, HMQC, and HMBC).
25659710	4	37	dep	H	831:831	arg1	TOCSY					847:851	TOCSY	847:851	TOCSY	847:851	After purified by Sepherdex G-25 column chromatography, the structural features of poly-mannose were investigated by high-performance anion-exchange chromatography (HPAEC), high-performance gel-permeation chromatography (HPGPC), infrared (IR) spectroscopy, methylation analysis and NMR spectroscopy analysis ((1)H, (13)C, COSY, TOCSY, HMQC, and HMBC).
25659710	6	38	theme	1→3	1205:1207	arg1	β-d-Manp					1217:1224	(1→3)-linked β-d-Manp	1204:1224	(1→3)-linked β-d-Manp	1204:1224	Methylation analysis and NMR spectroscopy revealed that the backbone of poly-mannose consisting of (1→3)-linked β-d-Manp, (1→3)-linked α-d-Manp, and (1→6)-linked α-d-Manp residues, and the main chain were branched at the O-2, O-3, O-4, O-6 position.
25659710	4	39	theme	high-performance	692:707	arg1	HPGPC					740:744	HPGPC	740:744	HPGPC	740:744	After purified by Sepherdex G-25 column chromatography, the structural features of poly-mannose were investigated by high-performance anion-exchange chromatography (HPAEC), high-performance gel-permeation chromatography (HPGPC), infrared (IR) spectroscopy, methylation analysis and NMR spectroscopy analysis ((1)H, (13)C, COSY, TOCSY, HMQC, and HMBC).
25659710	4	39	theme	high-performance	692:707	arg1	chromatography					724:737	high-performance gel-permeation chromatography	692:737	high-performance gel-permeation chromatography (HPGPC)	692:745	After purified by Sepherdex G-25 column chromatography, the structural features of poly-mannose were investigated by high-performance anion-exchange chromatography (HPAEC), high-performance gel-permeation chromatography (HPGPC), infrared (IR) spectroscopy, methylation analysis and NMR spectroscopy analysis ((1)H, (13)C, COSY, TOCSY, HMQC, and HMBC).
25659710	4	39	theme	high-performance	692:707	arg1	chromatography					668:681	high-performance anion-exchange chromatography	636:681	high-performance anion-exchange chromatography (HPAEC)	636:689	After purified by Sepherdex G-25 column chromatography, the structural features of poly-mannose were investigated by high-performance anion-exchange chromatography (HPAEC), high-performance gel-permeation chromatography (HPGPC), infrared (IR) spectroscopy, methylation analysis and NMR spectroscopy analysis ((1)H, (13)C, COSY, TOCSY, HMQC, and HMBC).
25659710	2	40	theme	2.5	433:435	arg1	mol/L					437:441	mol/L	437:441	mol/L	437:441	The optimum reaction conditions were microwave output power of 900 W, temperature 115°C, proton concentration 2.5 mol/L, and microwave irradiation time 5 min.
25659710	4	41	theme	structural	579:588	arg1	features					590:597	the structural features	575:597	the structural features of poly-mannose	575:613	After purified by Sepherdex G-25 column chromatography, the structural features of poly-mannose were investigated by high-performance anion-exchange chromatography (HPAEC), high-performance gel-permeation chromatography (HPGPC), infrared (IR) spectroscopy, methylation analysis and NMR spectroscopy analysis ((1)H, (13)C, COSY, TOCSY, HMQC, and HMBC).
25659710	4	42	theme	methylation	776:786	arg1	chromatography					668:681	high-performance anion-exchange chromatography	636:681	high-performance anion-exchange chromatography (HPAEC)	636:689	After purified by Sepherdex G-25 column chromatography, the structural features of poly-mannose were investigated by high-performance anion-exchange chromatography (HPAEC), high-performance gel-permeation chromatography (HPGPC), infrared (IR) spectroscopy, methylation analysis and NMR spectroscopy analysis ((1)H, (13)C, COSY, TOCSY, HMQC, and HMBC).
25659710	4	42	theme	methylation	776:786	arg1	analysis					788:795	methylation analysis	776:795	methylation analysis	776:795	After purified by Sepherdex G-25 column chromatography, the structural features of poly-mannose were investigated by high-performance anion-exchange chromatography (HPAEC), high-performance gel-permeation chromatography (HPGPC), infrared (IR) spectroscopy, methylation analysis and NMR spectroscopy analysis ((1)H, (13)C, COSY, TOCSY, HMQC, and HMBC).
25659710	4	43	theme	column	552:557	arg1	chromatography					559:572	Sepherdex G-25 column chromatography	537:572	Sepherdex G-25 column chromatography	537:572	After purified by Sepherdex G-25 column chromatography, the structural features of poly-mannose were investigated by high-performance anion-exchange chromatography (HPAEC), high-performance gel-permeation chromatography (HPGPC), infrared (IR) spectroscopy, methylation analysis and NMR spectroscopy analysis ((1)H, (13)C, COSY, TOCSY, HMQC, and HMBC).
25659710	4	44	theme	infrared	748:755	arg1	spectroscopy					762:773	infrared (IR) spectroscopy	748:773	infrared (IR) spectroscopy	748:773	After purified by Sepherdex G-25 column chromatography, the structural features of poly-mannose were investigated by high-performance anion-exchange chromatography (HPAEC), high-performance gel-permeation chromatography (HPGPC), infrared (IR) spectroscopy, methylation analysis and NMR spectroscopy analysis ((1)H, (13)C, COSY, TOCSY, HMQC, and HMBC).
25659710	4	44	theme	infrared	748:755	arg1	chromatography					668:681	high-performance anion-exchange chromatography	636:681	high-performance anion-exchange chromatography (HPAEC)	636:689	After purified by Sepherdex G-25 column chromatography, the structural features of poly-mannose were investigated by high-performance anion-exchange chromatography (HPAEC), high-performance gel-permeation chromatography (HPGPC), infrared (IR) spectroscopy, methylation analysis and NMR spectroscopy analysis ((1)H, (13)C, COSY, TOCSY, HMQC, and HMBC).
25659710	4	45	theme	G-25	547:550	arg1	chromatography					559:572	Sepherdex G-25 column chromatography	537:572	Sepherdex G-25 column chromatography	537:572	After purified by Sepherdex G-25 column chromatography, the structural features of poly-mannose were investigated by high-performance anion-exchange chromatography (HPAEC), high-performance gel-permeation chromatography (HPGPC), infrared (IR) spectroscopy, methylation analysis and NMR spectroscopy analysis ((1)H, (13)C, COSY, TOCSY, HMQC, and HMBC).
25659710	4	46	theme	poly-mannose	602:613	arg1	features					590:597	the structural features	575:597	the structural features of poly-mannose	575:613	After purified by Sepherdex G-25 column chromatography, the structural features of poly-mannose were investigated by high-performance anion-exchange chromatography (HPAEC), high-performance gel-permeation chromatography (HPGPC), infrared (IR) spectroscopy, methylation analysis and NMR spectroscopy analysis ((1)H, (13)C, COSY, TOCSY, HMQC, and HMBC).
25659710	6	47	theme	Methylation	1105:1115	arg1	analysis					1117:1124	Methylation analysis	1105:1124	Methylation analysis	1105:1124	Methylation analysis and NMR spectroscopy revealed that the backbone of poly-mannose consisting of (1→3)-linked β-d-Manp, (1→3)-linked α-d-Manp, and (1→6)-linked α-d-Manp residues, and the main chain were branched at the O-2, O-3, O-4, O-6 position.
25659710	1	48	theme	molecular	151:159	arg1	weight					161:166	molecular weight	151:166	molecular weight of 2.457 kDa	151:179	Poly-mannose with molecular weight of 2.457 kDa was synthesized using d-mannose as substrate and phosphoric acid as catalyst under the condition of microwave irradiation for the first time.
25659710	4	49	theme	Sepherdex	537:545	arg1	chromatography					559:572	Sepherdex G-25 column chromatography	537:572	Sepherdex G-25 column chromatography	537:572	After purified by Sepherdex G-25 column chromatography, the structural features of poly-mannose were investigated by high-performance anion-exchange chromatography (HPAEC), high-performance gel-permeation chromatography (HPGPC), infrared (IR) spectroscopy, methylation analysis and NMR spectroscopy analysis ((1)H, (13)C, COSY, TOCSY, HMQC, and HMBC).
25659710	6	50	theme	poly-mannose	1177:1188	arg1	backbone					1165:1172	the backbone	1161:1172	the backbone of poly-mannose consisting of (1→3)-linked β-d-Manp, (1→3)-linked α-d-Manp, and (1→6)-linked α-d-Manp residues, and the main chain	1161:1303	Methylation analysis and NMR spectroscopy revealed that the backbone of poly-mannose consisting of (1→3)-linked β-d-Manp, (1→3)-linked α-d-Manp, and (1→6)-linked α-d-Manp residues, and the main chain were branched at the O-2, O-3, O-4, O-6 position.
25659710	2	51	theme	microwave	360:368	arg1	power					377:381	microwave output power	360:381	microwave output power of 900 W	360:390	The optimum reaction conditions were microwave output power of 900 W, temperature 115°C, proton concentration 2.5 mol/L, and microwave irradiation time 5 min.
25659710	2	51	theme	microwave	360:368	arg1	conditions					344:353	The optimum reaction conditions	323:353	The optimum reaction conditions	323:353	The optimum reaction conditions were microwave output power of 900 W, temperature 115°C, proton concentration 2.5 mol/L, and microwave irradiation time 5 min.
25659710	2	52	dep	power	377:381	arg1	min					477:479	time 5 min	470:479	time 5 min	470:479	The optimum reaction conditions were microwave output power of 900 W, temperature 115°C, proton concentration 2.5 mol/L, and microwave irradiation time 5 min.
25659710	6	53	theme	NMR	1130:1132	arg1	spectroscopy					1134:1145	NMR spectroscopy	1130:1145	NMR spectroscopy	1130:1145	Methylation analysis and NMR spectroscopy revealed that the backbone of poly-mannose consisting of (1→3)-linked β-d-Manp, (1→3)-linked α-d-Manp, and (1→6)-linked α-d-Manp residues, and the main chain were branched at the O-2, O-3, O-4, O-6 position.
25659710	5	54	theme	single	1000:1005	arg1	peak					1025:1028	a single symmetrical sharp peak	998:1028	a single symmetrical sharp peak	998:1028	HPAEC analysis showed that the composition of synthetic polysaccharides was d-mannose, its purity was demonstrated by HPGPC as a single symmetrical sharp peak, and additionally IR spectra demonstrated the polymerization of d-mannose.
25659710	5	54	theme	single	1000:1005	arg1	purity					962:967	its purity	958:967	its purity	958:967	HPAEC analysis showed that the composition of synthetic polysaccharides was d-mannose, its purity was demonstrated by HPGPC as a single symmetrical sharp peak, and additionally IR spectra demonstrated the polymerization of d-mannose.
25659710	6	55	theme	-linked	1259:1265	arg1	residues					1276:1283	(1→6)-linked α-d-Manp residues	1254:1283	(1→6)-linked α-d-Manp residues	1254:1283	Methylation analysis and NMR spectroscopy revealed that the backbone of poly-mannose consisting of (1→3)-linked β-d-Manp, (1→3)-linked α-d-Manp, and (1→6)-linked α-d-Manp residues, and the main chain were branched at the O-2, O-3, O-4, O-6 position.
25659710	1	56	theme	kDa	177:179	arg1	weight					161:166	molecular weight	151:166	molecular weight of 2.457 kDa	151:179	Poly-mannose with molecular weight of 2.457 kDa was synthesized using d-mannose as substrate and phosphoric acid as catalyst under the condition of microwave irradiation for the first time.
25659710	3	57	theme	actual	486:491	arg1	yield					501:505	The actual maximum yield	482:505	The actual maximum yield	482:505	The actual maximum yield was 91.46%.
25659710	3	57	theme	actual	486:491	arg1	%					516:516	91.46%	511:516	91.46%	511:516	The actual maximum yield was 91.46%.
25659710	0	58	theme	acid	86:89	arg1	catalyzation					91:102	phosphoric acid catalyzation	75:102	phosphoric acid catalyzation under microwave irradiation	75:130	Preparation and structural characterization of poly-mannose synthesized by phosphoric acid catalyzation under microwave irradiation.
25659710	5	59	theme	symmetrical	1007:1017	arg1	peak					1025:1028	a single symmetrical sharp peak	998:1028	a single symmetrical sharp peak	998:1028	HPAEC analysis showed that the composition of synthetic polysaccharides was d-mannose, its purity was demonstrated by HPGPC as a single symmetrical sharp peak, and additionally IR spectra demonstrated the polymerization of d-mannose.
25659710	5	59	theme	symmetrical	1007:1017	arg1	purity					962:967	its purity	958:967	its purity	958:967	HPAEC analysis showed that the composition of synthetic polysaccharides was d-mannose, its purity was demonstrated by HPGPC as a single symmetrical sharp peak, and additionally IR spectra demonstrated the polymerization of d-mannose.
25659710	6	60	link	-linked	1259:1265	arg1	residues					1276:1283	(1→6)-linked α-d-Manp residues	1254:1283	(1→6)-linked α-d-Manp residues	1254:1283	Methylation analysis and NMR spectroscopy revealed that the backbone of poly-mannose consisting of (1→3)-linked β-d-Manp, (1→3)-linked α-d-Manp, and (1→6)-linked α-d-Manp residues, and the main chain were branched at the O-2, O-3, O-4, O-6 position.
25659710	4	61	theme	high-performance	636:651	arg1	chromatography					668:681	high-performance anion-exchange chromatography	636:681	high-performance anion-exchange chromatography (HPAEC)	636:689	After purified by Sepherdex G-25 column chromatography, the structural features of poly-mannose were investigated by high-performance anion-exchange chromatography (HPAEC), high-performance gel-permeation chromatography (HPGPC), infrared (IR) spectroscopy, methylation analysis and NMR spectroscopy analysis ((1)H, (13)C, COSY, TOCSY, HMQC, and HMBC).
25659710	4	61	theme	high-performance	636:651	arg1	analysis					788:795	methylation analysis	776:795	methylation analysis	776:795	After purified by Sepherdex G-25 column chromatography, the structural features of poly-mannose were investigated by high-performance anion-exchange chromatography (HPAEC), high-performance gel-permeation chromatography (HPGPC), infrared (IR) spectroscopy, methylation analysis and NMR spectroscopy analysis ((1)H, (13)C, COSY, TOCSY, HMQC, and HMBC).
25659710	4	61	theme	high-performance	636:651	arg1	chromatography					724:737	high-performance gel-permeation chromatography	692:737	high-performance gel-permeation chromatography (HPGPC)	692:745	After purified by Sepherdex G-25 column chromatography, the structural features of poly-mannose were investigated by high-performance anion-exchange chromatography (HPAEC), high-performance gel-permeation chromatography (HPGPC), infrared (IR) spectroscopy, methylation analysis and NMR spectroscopy analysis ((1)H, (13)C, COSY, TOCSY, HMQC, and HMBC).
25659710	4	61	theme	high-performance	636:651	arg1	HPAEC					684:688	HPAEC	684:688	HPAEC	684:688	After purified by Sepherdex G-25 column chromatography, the structural features of poly-mannose were investigated by high-performance anion-exchange chromatography (HPAEC), high-performance gel-permeation chromatography (HPGPC), infrared (IR) spectroscopy, methylation analysis and NMR spectroscopy analysis ((1)H, (13)C, COSY, TOCSY, HMQC, and HMBC).
25659710	4	61	theme	high-performance	636:651	arg1	spectroscopy					762:773	infrared (IR) spectroscopy	748:773	infrared (IR) spectroscopy	748:773	After purified by Sepherdex G-25 column chromatography, the structural features of poly-mannose were investigated by high-performance anion-exchange chromatography (HPAEC), high-performance gel-permeation chromatography (HPGPC), infrared (IR) spectroscopy, methylation analysis and NMR spectroscopy analysis ((1)H, (13)C, COSY, TOCSY, HMQC, and HMBC).
25659710	4	61	theme	high-performance	636:651	arg1	analysis					818:825	NMR spectroscopy analysis	801:825	NMR spectroscopy analysis	801:825	After purified by Sepherdex G-25 column chromatography, the structural features of poly-mannose were investigated by high-performance anion-exchange chromatography (HPAEC), high-performance gel-permeation chromatography (HPGPC), infrared (IR) spectroscopy, methylation analysis and NMR spectroscopy analysis ((1)H, (13)C, COSY, TOCSY, HMQC, and HMBC).
25659710	0	62	theme	phosphoric	75:84	arg1	catalyzation					91:102	phosphoric acid catalyzation	75:102	phosphoric acid catalyzation under microwave irradiation	75:130	Preparation and structural characterization of poly-mannose synthesized by phosphoric acid catalyzation under microwave irradiation.
25659710	2	63	theme	W	390:390	arg1	irradiation					458:468	microwave irradiation	448:468	microwave irradiation	448:468	The optimum reaction conditions were microwave output power of 900 W, temperature 115°C, proton concentration 2.5 mol/L, and microwave irradiation time 5 min.
25659710	2	63	theme	W	390:390	arg1	power					377:381	microwave output power	360:381	microwave output power of 900 W	360:390	The optimum reaction conditions were microwave output power of 900 W, temperature 115°C, proton concentration 2.5 mol/L, and microwave irradiation time 5 min.
25659710	2	63	theme	W	390:390	arg1	115°C					405:409	temperature 115°C	393:409	temperature 115°C	393:409	The optimum reaction conditions were microwave output power of 900 W, temperature 115°C, proton concentration 2.5 mol/L, and microwave irradiation time 5 min.
25659710	2	63	theme	W	390:390	arg1	concentration					419:431	proton concentration	412:431	proton concentration 2.5 mol/L	412:441	The optimum reaction conditions were microwave output power of 900 W, temperature 115°C, proton concentration 2.5 mol/L, and microwave irradiation time 5 min.
25659710	2	63	theme	W	390:390	arg1	conditions					344:353	The optimum reaction conditions	323:353	The optimum reaction conditions	323:353	The optimum reaction conditions were microwave output power of 900 W, temperature 115°C, proton concentration 2.5 mol/L, and microwave irradiation time 5 min.
24762570	0	0	theme	ferritin	87:94	arg1	spectroscopy					15:26	spectroscopy	15:26	spectroscopy	15:26	57Fe Mössbauer spectroscopy and electron paramagnetic resonance studies of human liver ferritin, Ferrum Lek and Maltofer®.
24762570	0	0	theme	ferritin	87:94	arg1	studies					64:70	electron paramagnetic resonance studies	32:70	electron paramagnetic resonance studies	32:70	57Fe Mössbauer spectroscopy and electron paramagnetic resonance studies of human liver ferritin, Ferrum Lek and Maltofer®.
24762570	1	1	theme	paramagnetic	246:257	arg1	resonance					259:267	electron paramagnetic resonance	237:267	electron paramagnetic resonance	237:267	A human liver ferritin, commercial Ferrum Lek and Maltofer® samples were studied using Mössbauer spectroscopy and electron paramagnetic resonance.
24762570	0	2	theme	liver	81:85	arg1	ferritin					87:94	human liver ferritin	75:94	human liver ferritin	75:94	57Fe Mössbauer spectroscopy and electron paramagnetic resonance studies of human liver ferritin, Ferrum Lek and Maltofer®.
24762570	1	3	theme	commercial	147:156	arg1	Lek					165:167	commercial Ferrum Lek	147:167	commercial Ferrum Lek	147:167	A human liver ferritin, commercial Ferrum Lek and Maltofer® samples were studied using Mössbauer spectroscopy and electron paramagnetic resonance.
24762570	3	4	contain	have	488:491	arg2	features					521:528	different superparamagnetic features	493:528	different superparamagnetic features	493:528	It is shown that the three studied materials have different superparamagnetic features at various temperatures.
24762570	3	4	contain	have	488:491	arg1	materials					478:486	the three studied materials	460:486	the three studied materials	460:486	It is shown that the three studied materials have different superparamagnetic features at various temperatures.
24762570	6	5	from	components	1013:1022	arg1	spectra					1040:1046	the measured spectra	1027:1046	the measured spectra which could be related to different regions, layers, nanocrystallites, etc. in the iron cores that coincides with heterogeneous and multiphase models for the ferritin iron cores	1027:1224	Mössbauer spectroscopy provides evidences on several components in the measured spectra which could be related to different regions, layers, nanocrystallites, etc. in the iron cores that coincides with heterogeneous and multiphase models for the ferritin iron cores.
24762570	6	5	from	components	1013:1022	arg1	related					1063:1069	related	1063:1069	related	1063:1069	Mössbauer spectroscopy provides evidences on several components in the measured spectra which could be related to different regions, layers, nanocrystallites, etc. in the iron cores that coincides with heterogeneous and multiphase models for the ferritin iron cores.
24762570	4	6	theme	iron	675:678	arg1	cores					680:684	the iron cores	671:684	the iron cores	671:684	This may be caused by different magnetic anisotropy energy barriers, sizes (volume), structures and compositions of the iron cores.
24762570	5	7	theme	ferro-	859:864	arg1	phases					883:888	minor ferro- or ferrimagnetic phases	853:888	minor ferro- or ferrimagnetic phases	853:888	The electron paramagnetic resonance spectra of the ferritin, Ferrum Lek and Maltofer® were decomposed into multiple spectral components demonstrating the presence of minor ferro- or ferrimagnetic phases along with revealing marked differences among the studied substances.
24762570	6	8	theme	iron	1215:1218	arg1	cores					1220:1224	the ferritin iron cores	1202:1224	the ferritin iron cores	1202:1224	Mössbauer spectroscopy provides evidences on several components in the measured spectra which could be related to different regions, layers, nanocrystallites, etc. in the iron cores that coincides with heterogeneous and multiphase models for the ferritin iron cores.
24762570	5	9	theme	Maltofer®	763:771	arg1	spectra					723:729	The electron paramagnetic resonance spectra	687:729	The electron paramagnetic resonance spectra of the ferritin, Ferrum Lek and Maltofer®	687:771	The electron paramagnetic resonance spectra of the ferritin, Ferrum Lek and Maltofer® were decomposed into multiple spectral components demonstrating the presence of minor ferro- or ferrimagnetic phases along with revealing marked differences among the studied substances.
24762570	6	10	theme	ferritin	1206:1213	arg1	cores					1220:1224	the ferritin iron cores	1202:1224	the ferritin iron cores	1202:1224	Mössbauer spectroscopy provides evidences on several components in the measured spectra which could be related to different regions, layers, nanocrystallites, etc. in the iron cores that coincides with heterogeneous and multiphase models for the ferritin iron cores.
24762570	6	11	theme	measured	1031:1038	arg1	spectra					1040:1046	the measured spectra	1027:1046	the measured spectra which could be related to different regions, layers, nanocrystallites, etc. in the iron cores that coincides with heterogeneous and multiphase models for the ferritin iron cores	1027:1224	Mössbauer spectroscopy provides evidences on several components in the measured spectra which could be related to different regions, layers, nanocrystallites, etc. in the iron cores that coincides with heterogeneous and multiphase models for the ferritin iron cores.
24762570	6	11	theme	measured	1031:1038	arg1	related					1063:1069	related	1063:1069	related	1063:1069	Mössbauer spectroscopy provides evidences on several components in the measured spectra which could be related to different regions, layers, nanocrystallites, etc. in the iron cores that coincides with heterogeneous and multiphase models for the ferritin iron cores.
24762570	1	12	theme	Ferrum	158:163	arg1	Lek					165:167	commercial Ferrum Lek	147:167	commercial Ferrum Lek	147:167	A human liver ferritin, commercial Ferrum Lek and Maltofer® samples were studied using Mössbauer spectroscopy and electron paramagnetic resonance.
24762570	0	13	theme	resonance	54:62	arg1	studies					64:70	electron paramagnetic resonance studies	32:70	electron paramagnetic resonance studies	32:70	57Fe Mössbauer spectroscopy and electron paramagnetic resonance studies of human liver ferritin, Ferrum Lek and Maltofer®.
24762570	6	14	from	evidences	992:1000	arg1	components					1013:1022	several components	1005:1022	several components in the measured spectra which could be related to different regions, layers, nanocrystallites, etc. in the iron cores that coincides with heterogeneous and multiphase models for the ferritin iron cores	1005:1224	Mössbauer spectroscopy provides evidences on several components in the measured spectra which could be related to different regions, layers, nanocrystallites, etc. in the iron cores that coincides with heterogeneous and multiphase models for the ferritin iron cores.
24762570	2	15	theme	Mössbauer	274:282	arg1	spectrometers					284:296	Two Mössbauer spectrometers	270:296	Two Mössbauer spectrometers	270:296	Two Mössbauer spectrometers have been used: (i) a high velocity resolution (4096 channels) at 90 and 295K, (ii) and a low velocity resolution (250 channels) at 20 and 40 K.
24762570	1	16	theme	Lek	165:167	arg1	samples					183:189	A human liver ferritin, commercial Ferrum Lek and Maltofer® samples	123:189	A human liver ferritin, commercial Ferrum Lek and Maltofer® samples	123:189	A human liver ferritin, commercial Ferrum Lek and Maltofer® samples were studied using Mössbauer spectroscopy and electron paramagnetic resonance.
24762570	2	17	theme	low	388:390	arg1	90					364:365	90	364:365	90	364:365	Two Mössbauer spectrometers have been used: (i) a high velocity resolution (4096 channels) at 90 and 295K, (ii) and a low velocity resolution (250 channels) at 20 and 40 K.
24762570	2	17	theme	low	388:390	arg1	resolution					401:410	a low velocity resolution	386:410	a low velocity resolution (250 channels)	386:425	Two Mössbauer spectrometers have been used: (i) a high velocity resolution (4096 channels) at 90 and 295K, (ii) and a low velocity resolution (250 channels) at 20 and 40 K.
24762570	2	17	theme	low	388:390	arg1	channels					417:424	250 channels	413:424	250 channels	413:424	Two Mössbauer spectrometers have been used: (i) a high velocity resolution (4096 channels) at 90 and 295K, (ii) and a low velocity resolution (250 channels) at 20 and 40 K.
24762570	3	18	theme	studied	470:476	arg1	materials					478:486	the three studied materials	460:486	the three studied materials	460:486	It is shown that the three studied materials have different superparamagnetic features at various temperatures.
24762570	4	19	theme	anisotropy	596:605	arg1	compositions					655:666	compositions	655:666	compositions	655:666	This may be caused by different magnetic anisotropy energy barriers, sizes (volume), structures and compositions of the iron cores.
24762570	4	19	theme	anisotropy	596:605	arg1	structures					640:649	structures	640:649	structures	640:649	This may be caused by different magnetic anisotropy energy barriers, sizes (volume), structures and compositions of the iron cores.
24762570	4	19	theme	anisotropy	596:605	arg1	sizes					624:628	sizes	624:628	sizes (volume)	624:637	This may be caused by different magnetic anisotropy energy barriers, sizes (volume), structures and compositions of the iron cores.
24762570	4	19	theme	anisotropy	596:605	arg1	barriers					614:621	different magnetic anisotropy energy barriers	577:621	different magnetic anisotropy energy barriers	577:621	This may be caused by different magnetic anisotropy energy barriers, sizes (volume), structures and compositions of the iron cores.
24762570	5	20	theme	marked	911:916	arg1	differences					918:928	marked differences	911:928	marked differences among the studied substances	911:957	The electron paramagnetic resonance spectra of the ferritin, Ferrum Lek and Maltofer® were decomposed into multiple spectral components demonstrating the presence of minor ferro- or ferrimagnetic phases along with revealing marked differences among the studied substances.
24762570	5	21	theme	spectral	803:810	arg1	components					812:821	multiple spectral components	794:821	multiple spectral components	794:821	The electron paramagnetic resonance spectra of the ferritin, Ferrum Lek and Maltofer® were decomposed into multiple spectral components demonstrating the presence of minor ferro- or ferrimagnetic phases along with revealing marked differences among the studied substances.
24762570	1	22	theme	Maltofer®	173:181	arg1	samples					183:189	A human liver ferritin, commercial Ferrum Lek and Maltofer® samples	123:189	A human liver ferritin, commercial Ferrum Lek and Maltofer® samples	123:189	A human liver ferritin, commercial Ferrum Lek and Maltofer® samples were studied using Mössbauer spectroscopy and electron paramagnetic resonance.
24762570	5	23	theme	minor	853:857	arg1	phases					883:888	minor ferro- or ferrimagnetic phases	853:888	minor ferro- or ferrimagnetic phases	853:888	The electron paramagnetic resonance spectra of the ferritin, Ferrum Lek and Maltofer® were decomposed into multiple spectral components demonstrating the presence of minor ferro- or ferrimagnetic phases along with revealing marked differences among the studied substances.
24762570	5	24	theme	ferrimagnetic	869:881	arg1	phases					883:888	minor ferro- or ferrimagnetic phases	853:888	minor ferro- or ferrimagnetic phases	853:888	The electron paramagnetic resonance spectra of the ferritin, Ferrum Lek and Maltofer® were decomposed into multiple spectral components demonstrating the presence of minor ferro- or ferrimagnetic phases along with revealing marked differences among the studied substances.
24762570	6	25	theme	multiphase	1180:1189	arg1	models					1191:1196	heterogeneous and multiphase models	1162:1196	heterogeneous and multiphase models for the ferritin iron cores	1162:1224	Mössbauer spectroscopy provides evidences on several components in the measured spectra which could be related to different regions, layers, nanocrystallites, etc. in the iron cores that coincides with heterogeneous and multiphase models for the ferritin iron cores.
24762570	6	26	theme	several	1005:1011	arg1	components					1013:1022	several components	1005:1022	several components in the measured spectra which could be related to different regions, layers, nanocrystallites, etc. in the iron cores that coincides with heterogeneous and multiphase models for the ferritin iron cores	1005:1224	Mössbauer spectroscopy provides evidences on several components in the measured spectra which could be related to different regions, layers, nanocrystallites, etc. in the iron cores that coincides with heterogeneous and multiphase models for the ferritin iron cores.
24762570	5	27	dep	demonstrating	823:835	arg1	revealing					901:909	revealing	901:909	revealing marked differences among the studied substances	901:957	The electron paramagnetic resonance spectra of the ferritin, Ferrum Lek and Maltofer® were decomposed into multiple spectral components demonstrating the presence of minor ferro- or ferrimagnetic phases along with revealing marked differences among the studied substances.
24762570	6	28	theme	iron	1131:1134	arg1	cores					1136:1140	the iron cores	1127:1140	the iron cores that coincides with heterogeneous and multiphase models for the ferritin iron cores	1127:1224	Mössbauer spectroscopy provides evidences on several components in the measured spectra which could be related to different regions, layers, nanocrystallites, etc. in the iron cores that coincides with heterogeneous and multiphase models for the ferritin iron cores.
24762570	5	29	theme	phases	883:888	arg1	presence					841:848	the presence	837:848	the presence of minor ferro- or ferrimagnetic phases	837:888	The electron paramagnetic resonance spectra of the ferritin, Ferrum Lek and Maltofer® were decomposed into multiple spectral components demonstrating the presence of minor ferro- or ferrimagnetic phases along with revealing marked differences among the studied substances.
24762570	5	30	theme	multiple	794:801	arg1	components					812:821	multiple spectral components	794:821	multiple spectral components	794:821	The electron paramagnetic resonance spectra of the ferritin, Ferrum Lek and Maltofer® were decomposed into multiple spectral components demonstrating the presence of minor ferro- or ferrimagnetic phases along with revealing marked differences among the studied substances.
24762570	0	31	theme	paramagnetic	41:52	arg1	studies					64:70	electron paramagnetic resonance studies	32:70	electron paramagnetic resonance studies	32:70	57Fe Mössbauer spectroscopy and electron paramagnetic resonance studies of human liver ferritin, Ferrum Lek and Maltofer®.
24762570	5	32	theme	studied	940:946	arg1	substances					948:957	the studied substances	936:957	the studied substances	936:957	The electron paramagnetic resonance spectra of the ferritin, Ferrum Lek and Maltofer® were decomposed into multiple spectral components demonstrating the presence of minor ferro- or ferrimagnetic phases along with revealing marked differences among the studied substances.
24762570	6	33	theme	different	1074:1082	arg1	etc.					1119:1122	etc.	1119:1122	etc.	1119:1122	Mössbauer spectroscopy provides evidences on several components in the measured spectra which could be related to different regions, layers, nanocrystallites, etc. in the iron cores that coincides with heterogeneous and multiphase models for the ferritin iron cores.
24762570	6	33	theme	different	1074:1082	arg1	nanocrystallites					1101:1116	nanocrystallites	1101:1116	nanocrystallites	1101:1116	Mössbauer spectroscopy provides evidences on several components in the measured spectra which could be related to different regions, layers, nanocrystallites, etc. in the iron cores that coincides with heterogeneous and multiphase models for the ferritin iron cores.
24762570	6	33	theme	different	1074:1082	arg1	layers					1093:1098	layers	1093:1098	layers	1093:1098	Mössbauer spectroscopy provides evidences on several components in the measured spectra which could be related to different regions, layers, nanocrystallites, etc. in the iron cores that coincides with heterogeneous and multiphase models for the ferritin iron cores.
24762570	6	33	theme	different	1074:1082	arg1	regions					1084:1090	different regions	1074:1090	different regions	1074:1090	Mössbauer spectroscopy provides evidences on several components in the measured spectra which could be related to different regions, layers, nanocrystallites, etc. in the iron cores that coincides with heterogeneous and multiphase models for the ferritin iron cores.
24762570	3	34	theme	superparamagnetic	503:519	arg1	features					521:528	different superparamagnetic features	493:528	different superparamagnetic features	493:528	It is shown that the three studied materials have different superparamagnetic features at various temperatures.
24762570	4	35	theme	magnetic	587:594	arg1	compositions					655:666	compositions	655:666	compositions	655:666	This may be caused by different magnetic anisotropy energy barriers, sizes (volume), structures and compositions of the iron cores.
24762570	4	35	theme	magnetic	587:594	arg1	structures					640:649	structures	640:649	structures	640:649	This may be caused by different magnetic anisotropy energy barriers, sizes (volume), structures and compositions of the iron cores.
24762570	4	35	theme	magnetic	587:594	arg1	sizes					624:628	sizes	624:628	sizes (volume)	624:637	This may be caused by different magnetic anisotropy energy barriers, sizes (volume), structures and compositions of the iron cores.
24762570	4	35	theme	magnetic	587:594	arg1	barriers					614:621	different magnetic anisotropy energy barriers	577:621	different magnetic anisotropy energy barriers	577:621	This may be caused by different magnetic anisotropy energy barriers, sizes (volume), structures and compositions of the iron cores.
24762570	5	36	theme	Lek	755:757	arg1	spectra					723:729	The electron paramagnetic resonance spectra	687:729	The electron paramagnetic resonance spectra of the ferritin, Ferrum Lek and Maltofer®	687:771	The electron paramagnetic resonance spectra of the ferritin, Ferrum Lek and Maltofer® were decomposed into multiple spectral components demonstrating the presence of minor ferro- or ferrimagnetic phases along with revealing marked differences among the studied substances.
24762570	4	37	theme	different	577:585	arg1	compositions					655:666	compositions	655:666	compositions	655:666	This may be caused by different magnetic anisotropy energy barriers, sizes (volume), structures and compositions of the iron cores.
24762570	4	37	theme	different	577:585	arg1	structures					640:649	structures	640:649	structures	640:649	This may be caused by different magnetic anisotropy energy barriers, sizes (volume), structures and compositions of the iron cores.
24762570	4	37	theme	different	577:585	arg1	sizes					624:628	sizes	624:628	sizes (volume)	624:637	This may be caused by different magnetic anisotropy energy barriers, sizes (volume), structures and compositions of the iron cores.
24762570	4	37	theme	different	577:585	arg1	barriers					614:621	different magnetic anisotropy energy barriers	577:621	different magnetic anisotropy energy barriers	577:621	This may be caused by different magnetic anisotropy energy barriers, sizes (volume), structures and compositions of the iron cores.
24762570	5	38	theme	electron	691:698	arg1	resonance					713:721	electron paramagnetic resonance	691:721	The electron paramagnetic resonance spectra of the ferritin, Ferrum Lek and Maltofer®	687:771	The electron paramagnetic resonance spectra of the ferritin, Ferrum Lek and Maltofer® were decomposed into multiple spectral components demonstrating the presence of minor ferro- or ferrimagnetic phases along with revealing marked differences among the studied substances.
24762570	5	39	theme	paramagnetic	700:711	arg1	resonance					713:721	electron paramagnetic resonance	691:721	The electron paramagnetic resonance spectra of the ferritin, Ferrum Lek and Maltofer®	687:771	The electron paramagnetic resonance spectra of the ferritin, Ferrum Lek and Maltofer® were decomposed into multiple spectral components demonstrating the presence of minor ferro- or ferrimagnetic phases along with revealing marked differences among the studied substances.
24762570	5	40	theme	resonance	713:721	arg1	spectra					723:729	The electron paramagnetic resonance spectra	687:729	The electron paramagnetic resonance spectra of the ferritin, Ferrum Lek and Maltofer®	687:771	The electron paramagnetic resonance spectra of the ferritin, Ferrum Lek and Maltofer® were decomposed into multiple spectral components demonstrating the presence of minor ferro- or ferrimagnetic phases along with revealing marked differences among the studied substances.
24762570	6	41	theme	heterogeneous	1162:1174	arg1	models					1191:1196	heterogeneous and multiphase models	1162:1196	heterogeneous and multiphase models for the ferritin iron cores	1162:1224	Mössbauer spectroscopy provides evidences on several components in the measured spectra which could be related to different regions, layers, nanocrystallites, etc. in the iron cores that coincides with heterogeneous and multiphase models for the ferritin iron cores.
24762570	6	42	from	related	1063:1069	arg1	cores					1136:1140	the iron cores	1127:1140	the iron cores that coincides with heterogeneous and multiphase models for the ferritin iron cores	1127:1224	Mössbauer spectroscopy provides evidences on several components in the measured spectra which could be related to different regions, layers, nanocrystallites, etc. in the iron cores that coincides with heterogeneous and multiphase models for the ferritin iron cores.
24762570	2	43	theme	velocity	325:332	arg1	resolution					334:343	a high velocity resolution	318:343	a high velocity resolution (4096 channels)	318:359	Two Mössbauer spectrometers have been used: (i) a high velocity resolution (4096 channels) at 90 and 295K, (ii) and a low velocity resolution (250 channels) at 20 and 40 K.
24762570	2	43	theme	velocity	325:332	arg1	channels					351:358	4096 channels	346:358	4096 channels	346:358	Two Mössbauer spectrometers have been used: (i) a high velocity resolution (4096 channels) at 90 and 295K, (ii) and a low velocity resolution (250 channels) at 20 and 40 K.
24762570	2	44	theme	velocity	392:399	arg1	90					364:365	90	364:365	90	364:365	Two Mössbauer spectrometers have been used: (i) a high velocity resolution (4096 channels) at 90 and 295K, (ii) and a low velocity resolution (250 channels) at 20 and 40 K.
24762570	2	44	theme	velocity	392:399	arg1	resolution					401:410	a low velocity resolution	386:410	a low velocity resolution (250 channels)	386:425	Two Mössbauer spectrometers have been used: (i) a high velocity resolution (4096 channels) at 90 and 295K, (ii) and a low velocity resolution (250 channels) at 20 and 40 K.
24762570	2	44	theme	velocity	392:399	arg1	channels					417:424	250 channels	413:424	250 channels	413:424	Two Mössbauer spectrometers have been used: (i) a high velocity resolution (4096 channels) at 90 and 295K, (ii) and a low velocity resolution (250 channels) at 20 and 40 K.
24762570	3	45	theme	different	493:501	arg1	features					521:528	different superparamagnetic features	493:528	different superparamagnetic features	493:528	It is shown that the three studied materials have different superparamagnetic features at various temperatures.
24762570	2	46	theme	high	320:323	arg1	resolution					334:343	a high velocity resolution	318:343	a high velocity resolution (4096 channels)	318:359	Two Mössbauer spectrometers have been used: (i) a high velocity resolution (4096 channels) at 90 and 295K, (ii) and a low velocity resolution (250 channels) at 20 and 40 K.
24762570	2	46	theme	high	320:323	arg1	channels					351:358	4096 channels	346:358	4096 channels	346:358	Two Mössbauer spectrometers have been used: (i) a high velocity resolution (4096 channels) at 90 and 295K, (ii) and a low velocity resolution (250 channels) at 20 and 40 K.
24762570	2	47	used	used	308:311	arg2	spectrometers					284:296	Two Mössbauer spectrometers	270:296	Two Mössbauer spectrometers	270:296	Two Mössbauer spectrometers have been used: (i) a high velocity resolution (4096 channels) at 90 and 295K, (ii) and a low velocity resolution (250 channels) at 20 and 40 K.
24762570	3	48	theme	various	533:539	arg1	temperatures					541:552	various temperatures	533:552	various temperatures	533:552	It is shown that the three studied materials have different superparamagnetic features at various temperatures.
24762570	5	49	dep	Lek	755:757	arg1	the					734:736	the	734:736	the	734:736	The electron paramagnetic resonance spectra of the ferritin, Ferrum Lek and Maltofer® were decomposed into multiple spectral components demonstrating the presence of minor ferro- or ferrimagnetic phases along with revealing marked differences among the studied substances.
24762570	5	49	dep	Lek	755:757	arg1	Ferrum					748:753	Ferrum	748:753	Ferrum	748:753	The electron paramagnetic resonance spectra of the ferritin, Ferrum Lek and Maltofer® were decomposed into multiple spectral components demonstrating the presence of minor ferro- or ferrimagnetic phases along with revealing marked differences among the studied substances.
24762570	4	50	theme	energy	607:612	arg1	compositions					655:666	compositions	655:666	compositions	655:666	This may be caused by different magnetic anisotropy energy barriers, sizes (volume), structures and compositions of the iron cores.
24762570	4	50	theme	energy	607:612	arg1	structures					640:649	structures	640:649	structures	640:649	This may be caused by different magnetic anisotropy energy barriers, sizes (volume), structures and compositions of the iron cores.
24762570	4	50	theme	energy	607:612	arg1	sizes					624:628	sizes	624:628	sizes (volume)	624:637	This may be caused by different magnetic anisotropy energy barriers, sizes (volume), structures and compositions of the iron cores.
24762570	4	50	theme	energy	607:612	arg1	barriers					614:621	different magnetic anisotropy energy barriers	577:621	different magnetic anisotropy energy barriers	577:621	This may be caused by different magnetic anisotropy energy barriers, sizes (volume), structures and compositions of the iron cores.
24762570	1	51	theme	Mössbauer	210:218	arg1	spectroscopy					220:231	Mössbauer spectroscopy	210:231	Mössbauer spectroscopy	210:231	A human liver ferritin, commercial Ferrum Lek and Maltofer® samples were studied using Mössbauer spectroscopy and electron paramagnetic resonance.
24762570	4	52	theme	cores	680:684	arg1	compositions					655:666	compositions	655:666	compositions	655:666	This may be caused by different magnetic anisotropy energy barriers, sizes (volume), structures and compositions of the iron cores.
24762570	4	52	theme	cores	680:684	arg1	structures					640:649	structures	640:649	structures	640:649	This may be caused by different magnetic anisotropy energy barriers, sizes (volume), structures and compositions of the iron cores.
24762570	4	52	theme	cores	680:684	arg1	barriers					614:621	different magnetic anisotropy energy barriers	577:621	different magnetic anisotropy energy barriers	577:621	This may be caused by different magnetic anisotropy energy barriers, sizes (volume), structures and compositions of the iron cores.
24762570	1	53	theme	human	125:129	arg1	ferritin					137:144	A human liver ferritin	123:144	A human liver ferritin	123:144	A human liver ferritin, commercial Ferrum Lek and Maltofer® samples were studied using Mössbauer spectroscopy and electron paramagnetic resonance.
24762570	6	54	theme	Mössbauer	960:968	arg1	spectroscopy					970:981	Mössbauer spectroscopy	960:981	Mössbauer spectroscopy	960:981	Mössbauer spectroscopy provides evidences on several components in the measured spectra which could be related to different regions, layers, nanocrystallites, etc. in the iron cores that coincides with heterogeneous and multiphase models for the ferritin iron cores.
24762570	0	55	theme	human	75:79	arg1	ferritin					87:94	human liver ferritin	75:94	human liver ferritin	75:94	57Fe Mössbauer spectroscopy and electron paramagnetic resonance studies of human liver ferritin, Ferrum Lek and Maltofer®.
24762570	2	56	from	K	440:440	arg1	90					364:365	90	364:365	90	364:365	Two Mössbauer spectrometers have been used: (i) a high velocity resolution (4096 channels) at 90 and 295K, (ii) and a low velocity resolution (250 channels) at 20 and 40 K.
24762570	2	56	from	K	440:440	arg1	resolution					401:410	a low velocity resolution	386:410	a low velocity resolution (250 channels)	386:425	Two Mössbauer spectrometers have been used: (i) a high velocity resolution (4096 channels) at 90 and 295K, (ii) and a low velocity resolution (250 channels) at 20 and 40 K.
24762570	2	56	from	K	440:440	arg1	ii					378:379	ii	378:379	ii	378:379	Two Mössbauer spectrometers have been used: (i) a high velocity resolution (4096 channels) at 90 and 295K, (ii) and a low velocity resolution (250 channels) at 20 and 40 K.
24762570	0	57	dep	Mössbauer	5:13	arg1	spectroscopy					15:26	spectroscopy	15:26	spectroscopy	15:26	57Fe Mössbauer spectroscopy and electron paramagnetic resonance studies of human liver ferritin, Ferrum Lek and Maltofer®.
24762570	0	57	dep	Mössbauer	5:13	arg1	studies					64:70	electron paramagnetic resonance studies	32:70	electron paramagnetic resonance studies	32:70	57Fe Mössbauer spectroscopy and electron paramagnetic resonance studies of human liver ferritin, Ferrum Lek and Maltofer®.
24762570	1	58	theme	liver	131:135	arg1	ferritin					137:144	A human liver ferritin	123:144	A human liver ferritin	123:144	A human liver ferritin, commercial Ferrum Lek and Maltofer® samples were studied using Mössbauer spectroscopy and electron paramagnetic resonance.
24762570	2	59	dep	90	364:365	arg1	i					315:315	i	315:315	i	315:315	Two Mössbauer spectrometers have been used: (i) a high velocity resolution (4096 channels) at 90 and 295K, (ii) and a low velocity resolution (250 channels) at 20 and 40 K.
24762570	1	60	theme	ferritin	137:144	arg1	samples					183:189	A human liver ferritin, commercial Ferrum Lek and Maltofer® samples	123:189	A human liver ferritin, commercial Ferrum Lek and Maltofer® samples	123:189	A human liver ferritin, commercial Ferrum Lek and Maltofer® samples were studied using Mössbauer spectroscopy and electron paramagnetic resonance.
24762570	6	61	from	cores	1136:1140	arg1	spectra					1040:1046	the measured spectra	1027:1046	the measured spectra which could be related to different regions, layers, nanocrystallites, etc. in the iron cores that coincides with heterogeneous and multiphase models for the ferritin iron cores	1027:1224	Mössbauer spectroscopy provides evidences on several components in the measured spectra which could be related to different regions, layers, nanocrystallites, etc. in the iron cores that coincides with heterogeneous and multiphase models for the ferritin iron cores.
24762570	6	61	from	cores	1136:1140	arg1	related					1063:1069	related	1063:1069	related	1063:1069	Mössbauer spectroscopy provides evidences on several components in the measured spectra which could be related to different regions, layers, nanocrystallites, etc. in the iron cores that coincides with heterogeneous and multiphase models for the ferritin iron cores.
24762570	1	62	theme	electron	237:244	arg1	resonance					259:267	electron paramagnetic resonance	237:267	electron paramagnetic resonance	237:267	A human liver ferritin, commercial Ferrum Lek and Maltofer® samples were studied using Mössbauer spectroscopy and electron paramagnetic resonance.
28231388	0	0	theme	meyenii	77:83	arg1	Maca					62:65	Maca	62:65	Maca (Lepidium meyenii Walp) Polysaccharide	62:104	Structural Characterization and Antifatigue Effect In Vivo of Maca (Lepidium meyenii Walp) Polysaccharide.
28231388	0	0	theme	meyenii	77:83	arg1	Walp					85:88	Lepidium meyenii Walp	68:88	Lepidium meyenii Walp	68:88	Structural Characterization and Antifatigue Effect In Vivo of Maca (Lepidium meyenii Walp) Polysaccharide.
28231388	7	1	theme	biochemical	1053:1063	arg1	parameters					1065:1074	the serous biochemical parameters	1042:1074	the serous biochemical parameters of mice	1042:1082	MP (above mid-dosage 50 mg/kg bw/d) could effectively elongate swimming durations and accelerate average swimming speeds (within the 1st 5 min) of mice (P < 0.05) and improve the serous biochemical parameters of mice.
28231388	1	2	with	polysaccharides	136:150	arg1	purity					162:167	purity	162:167	purity of 99.2%	162:176	Maca (Lepidium meyenii Walp) polysaccharides (MP) with purity of 99.2% were obtained to investigate their structural characteristics and antifatigue effect in vivo.
28231388	2	3	theme	gel	348:350	arg1	chromatography					363:376	high-performance gel filtration chromatography	331:376	high-performance gel filtration chromatography	331:376	The physicochemical properties of MP were analyzed through high-performance gel filtration chromatography, IR, monosaccharide composition, methylation, GC-MS, and NMR analyses.
28231388	1	4	theme	structural	213:222	arg1	characteristics					224:238	their structural characteristics	207:238	their structural characteristics	207:238	Maca (Lepidium meyenii Walp) polysaccharides (MP) with purity of 99.2% were obtained to investigate their structural characteristics and antifatigue effect in vivo.
28231388	0	5	theme	Lepidium	68:75	arg1	Maca					62:65	Maca	62:65	Maca (Lepidium meyenii Walp) Polysaccharide	62:104	Structural Characterization and Antifatigue Effect In Vivo of Maca (Lepidium meyenii Walp) Polysaccharide.
28231388	0	5	theme	Lepidium	68:75	arg1	Walp					85:88	Lepidium meyenii Walp	68:88	Lepidium meyenii Walp	68:88	Structural Characterization and Antifatigue Effect In Vivo of Maca (Lepidium meyenii Walp) Polysaccharide.
28231388	10	6	with	polysaccharide	1448:1461	arg1	responsible					1506:1516	responsible	1506:1516	responsible	1506:1516	MP is an acidic polysaccharide with a high D-GalA content, which could be responsible for the antifatigue effect of maca.
28231388	10	6	with	polysaccharide	1448:1461	arg1	content					1482:1488	a high D-GalA content	1468:1488	a high D-GalA content	1468:1488	MP is an acidic polysaccharide with a high D-GalA content, which could be responsible for the antifatigue effect of maca.
28231388	10	7	theme	high	1470:1473	arg1	responsible					1506:1516	responsible	1506:1516	responsible	1506:1516	MP is an acidic polysaccharide with a high D-GalA content, which could be responsible for the antifatigue effect of maca.
28231388	10	7	theme	high	1470:1473	arg1	content					1482:1488	a high D-GalA content	1468:1488	a high D-GalA content	1468:1488	MP is an acidic polysaccharide with a high D-GalA content, which could be responsible for the antifatigue effect of maca.
28231388	2	8	theme	high-performance	331:346	arg1	chromatography					363:376	high-performance gel filtration chromatography	331:376	high-performance gel filtration chromatography	331:376	The physicochemical properties of MP were analyzed through high-performance gel filtration chromatography, IR, monosaccharide composition, methylation, GC-MS, and NMR analyses.
28231388	9	9	theme	High-dosage	1310:1320	arg1	MP					1322:1323	High-dosage MP	1310:1323	High-dosage MP	1310:1323	High-dosage MP could significantly reduce the levels of blood urea nitrogen, lactic acid, and malondialdehyde (P < 0.05).
28231388	4	10	theme	acidic	548:553	arg1	MP					539:540	MP	539:540	MP	539:540	MP is an acidic heteropolysaccharide with an average molecular weight (Mw ) of 793.5 kDa.
28231388	4	10	theme	acidic	548:553	arg1	heteropolysaccharide					555:574	an acidic heteropolysaccharide	545:574	an acidic heteropolysaccharide with an average molecular weight (Mw ) of 793.5 kDa	545:626	MP is an acidic heteropolysaccharide with an average molecular weight (Mw ) of 793.5 kDa.
28231388	5	11	dep	D-GalA	647:652	arg1	%					731:731	mol, %	726:731	%	731:731	It is composed of D-GalA: D-Glc: L-Ara: D-Man: D-Gal: L-Rha = 35.07:29.98:16.98:13.01:4.21:0.75 (mol, %).
28231388	5	11	dep	D-GalA	647:652	arg1	L-Ara					662:666	L-Ara	662:666	L-Ara	662:666	It is composed of D-GalA: D-Glc: L-Ara: D-Man: D-Gal: L-Rha = 35.07:29.98:16.98:13.01:4.21:0.75 (mol, %).
28231388	5	11	dep	D-GalA	647:652	arg1	35.07:29.98:16.98:13.01:4.21:0.75					691:723	35.07:29.98:16.98:13.01:4.21:0.75	691:723	35.07:29.98:16.98:13.01:4.21:0.75	691:723	It is composed of D-GalA: D-Glc: L-Ara: D-Man: D-Gal: L-Rha = 35.07:29.98:16.98:13.01:4.21:0.75 (mol, %).
28231388	5	11	dep	D-GalA	647:652	arg1	D-Man					669:673	D-Man	669:673	D-Man	669:673	It is composed of D-GalA: D-Glc: L-Ara: D-Man: D-Gal: L-Rha = 35.07:29.98:16.98:13.01:4.21:0.75 (mol, %).
28231388	5	11	dep	D-GalA	647:652	arg1	D-Gal					676:680	D-Gal	676:680	D-Gal	676:680	It is composed of D-GalA: D-Glc: L-Ara: D-Man: D-Gal: L-Rha = 35.07:29.98:16.98:13.01:4.21:0.75 (mol, %).
28231388	5	11	dep	D-GalA	647:652	arg1	D-Glc					655:659	D-Glc	655:659	D-GalA: D-Glc: L-Ara: D-Man: D-Gal: L-Rha = 35.07:29.98:16.98:13.01:4.21:0.75 (mol, %)	647:732	It is composed of D-GalA: D-Glc: L-Ara: D-Man: D-Gal: L-Rha = 35.07:29.98:16.98:13.01:4.21:0.75 (mol, %).
28231388	3	12	theme	swimming	523:530	arg1	model					532:536	a mouse weight-loaded swimming model	501:536	a mouse weight-loaded swimming model	501:536	The antifatigue effect of MP was evaluated by using a mouse weight-loaded swimming model.
28231388	9	13	theme	nitrogen	1377:1384	arg1	levels					1356:1361	the levels	1352:1361	the levels of blood urea nitrogen, lactic acid, and malondialdehyde (P < 0.05)	1352:1429	High-dosage MP could significantly reduce the levels of blood urea nitrogen, lactic acid, and malondialdehyde (P < 0.05).
28231388	7	14	theme	serous	1046:1051	arg1	parameters					1065:1074	the serous biochemical parameters	1042:1074	the serous biochemical parameters of mice	1042:1082	MP (above mid-dosage 50 mg/kg bw/d) could effectively elongate swimming durations and accelerate average swimming speeds (within the 1st 5 min) of mice (P < 0.05) and improve the serous biochemical parameters of mice.
28231388	10	15	theme	antifatigue	1526:1536	arg1	effect					1538:1543	the antifatigue effect	1522:1543	the antifatigue effect of maca	1522:1551	MP is an acidic polysaccharide with a high D-GalA content, which could be responsible for the antifatigue effect of maca.
28231388	7	16	theme	1st	1000:1002	arg1	min					1006:1008	the 1st 5 min	996:1008	the 1st 5 min	996:1008	MP (above mid-dosage 50 mg/kg bw/d) could effectively elongate swimming durations and accelerate average swimming speeds (within the 1st 5 min) of mice (P < 0.05) and improve the serous biochemical parameters of mice.
28231388	6	17	contain	contained	765:773	arg2	β-1,3-Glcp					790:799	β-1,3-Glcp	790:799	β-1,3-Glcp	790:799	The findings revealed that MP contained β-1,3-Galp(A), β-1,3-Glcp, and α-1, 3-Manp linked alternatingly to form a backbone (5:4:1).
28231388	6	17	contain	contained	765:773	arg2	A					786:786	A	786:786	A	786:786	The findings revealed that MP contained β-1,3-Galp(A), β-1,3-Glcp, and α-1, 3-Manp linked alternatingly to form a backbone (5:4:1).
28231388	6	17	contain	contained	765:773	arg2	α-1					806:808	α-1	806:808	α-1	806:808	The findings revealed that MP contained β-1,3-Galp(A), β-1,3-Glcp, and α-1, 3-Manp linked alternatingly to form a backbone (5:4:1).
28231388	6	17	contain	contained	765:773	arg1	MP					762:763	MP	762:763	MP	762:763	The findings revealed that MP contained β-1,3-Galp(A), β-1,3-Glcp, and α-1, 3-Manp linked alternatingly to form a backbone (5:4:1).
28231388	6	17	contain	contained	765:773	arg2	3-Manp					811:816	3-Manp	811:816	3-Manp linked alternatingly to form a backbone (5:4:1)	811:864	The findings revealed that MP contained β-1,3-Galp(A), β-1,3-Glcp, and α-1, 3-Manp linked alternatingly to form a backbone (5:4:1).
28231388	6	17	contain	contained	765:773	arg2	β-1,3-Galp					775:784	β-1,3-Galp	775:784	β-1,3-Galp(A)	775:787	The findings revealed that MP contained β-1,3-Galp(A), β-1,3-Glcp, and α-1, 3-Manp linked alternatingly to form a backbone (5:4:1).
28231388	2	18	theme	MP	306:307	arg1	properties					292:301	The physicochemical properties	272:301	The physicochemical properties of MP	272:307	The physicochemical properties of MP were analyzed through high-performance gel filtration chromatography, IR, monosaccharide composition, methylation, GC-MS, and NMR analyses.
28231388	1	19	theme	Maca	107:110	arg1	MP					153:154	MP	153:154	MP	153:154	Maca (Lepidium meyenii Walp) polysaccharides (MP) with purity of 99.2% were obtained to investigate their structural characteristics and antifatigue effect in vivo.
28231388	1	19	theme	Maca	107:110	arg1	polysaccharides					136:150	Maca (Lepidium meyenii Walp) polysaccharides	107:150	Maca (Lepidium meyenii Walp) polysaccharides (MP) with purity of 99.2%	107:176	Maca (Lepidium meyenii Walp) polysaccharides (MP) with purity of 99.2% were obtained to investigate their structural characteristics and antifatigue effect in vivo.
28231388	8	20	theme	control	1103:1109	arg1	model					1111:1115	the control model	1099:1115	the control model	1099:1115	Compared with the control model, high-dosage (100 mg/kg bw/d) MP treatment could significantly enhance glutathione peroxidase and creatine kinase activities (P < 0.05) and decreased lactate dehydrogenase activity (P < 0.01).
28231388	3	21	theme	mouse	503:507	arg1	model					532:536	a mouse weight-loaded swimming model	501:536	a mouse weight-loaded swimming model	501:536	The antifatigue effect of MP was evaluated by using a mouse weight-loaded swimming model.
28231388	2	22	theme	monosaccharide	383:396	arg1	composition					398:408	monosaccharide composition	383:408	monosaccharide composition	383:408	The physicochemical properties of MP were analyzed through high-performance gel filtration chromatography, IR, monosaccharide composition, methylation, GC-MS, and NMR analyses.
28231388	0	23	theme	Polysaccharide	91:104	arg1	Characterization					11:26	Structural Characterization	0:26	Structural Characterization	0:26	Structural Characterization and Antifatigue Effect In Vivo of Maca (Lepidium meyenii Walp) Polysaccharide.
28231388	0	23	theme	Polysaccharide	91:104	arg1	Effect					44:49	Antifatigue Effect	32:49	Antifatigue Effect	32:49	Structural Characterization and Antifatigue Effect In Vivo of Maca (Lepidium meyenii Walp) Polysaccharide.
28231388	7	24	theme	mice	1014:1017	arg1	speeds					981:986	average swimming speeds	964:986	average swimming speeds (within the 1st 5 min) of mice (P < 0.05)	964:1028	MP (above mid-dosage 50 mg/kg bw/d) could effectively elongate swimming durations and accelerate average swimming speeds (within the 1st 5 min) of mice (P < 0.05) and improve the serous biochemical parameters of mice.
28231388	3	25	theme	weight-loaded	509:521	arg1	model					532:536	a mouse weight-loaded swimming model	501:536	a mouse weight-loaded swimming model	501:536	The antifatigue effect of MP was evaluated by using a mouse weight-loaded swimming model.
28231388	4	26	theme	kDa	624:626	arg1	Mw					610:611	Mw	610:611	Mw	610:611	MP is an acidic heteropolysaccharide with an average molecular weight (Mw ) of 793.5 kDa.
28231388	4	26	theme	kDa	624:626	arg1	weight					602:607	an average molecular weight	581:607	an average molecular weight (Mw ) of 793.5 kDa	581:626	MP is an acidic heteropolysaccharide with an average molecular weight (Mw ) of 793.5 kDa.
28231388	2	27	theme	NMR	435:437	arg1	analyses					439:446	NMR analyses	435:446	NMR analyses	435:446	The physicochemical properties of MP were analyzed through high-performance gel filtration chromatography, IR, monosaccharide composition, methylation, GC-MS, and NMR analyses.
28231388	7	28	theme	swimming	972:979	arg1	speeds					981:986	average swimming speeds	964:986	average swimming speeds (within the 1st 5 min) of mice (P < 0.05)	964:1028	MP (above mid-dosage 50 mg/kg bw/d) could effectively elongate swimming durations and accelerate average swimming speeds (within the 1st 5 min) of mice (P < 0.05) and improve the serous biochemical parameters of mice.
28231388	8	29	theme	creatine	1215:1222	arg1	kinase					1224:1229	creatine kinase	1215:1229	creatine kinase	1215:1229	Compared with the control model, high-dosage (100 mg/kg bw/d) MP treatment could significantly enhance glutathione peroxidase and creatine kinase activities (P < 0.05) and decreased lactate dehydrogenase activity (P < 0.01).
28231388	0	30	theme	Structural	0:9	arg1	Characterization					11:26	Structural Characterization	0:26	Structural Characterization	0:26	Structural Characterization and Antifatigue Effect In Vivo of Maca (Lepidium meyenii Walp) Polysaccharide.
28231388	9	31	theme	acid	1394:1397	arg1	levels					1356:1361	the levels	1352:1361	the levels of blood urea nitrogen, lactic acid, and malondialdehyde (P < 0.05)	1352:1429	High-dosage MP could significantly reduce the levels of blood urea nitrogen, lactic acid, and malondialdehyde (P < 0.05).
28231388	8	32	theme	peroxidase	1200:1209	arg1	<					1245:1245	P < 0.05	1243:1250	P < 0.05	1243:1250	Compared with the control model, high-dosage (100 mg/kg bw/d) MP treatment could significantly enhance glutathione peroxidase and creatine kinase activities (P < 0.05) and decreased lactate dehydrogenase activity (P < 0.01).
28231388	8	32	theme	peroxidase	1200:1209	arg1	activities					1231:1240	glutathione peroxidase and creatine kinase activities	1188:1240	glutathione peroxidase and creatine kinase activities (P < 0.05)	1188:1251	Compared with the control model, high-dosage (100 mg/kg bw/d) MP treatment could significantly enhance glutathione peroxidase and creatine kinase activities (P < 0.05) and decreased lactate dehydrogenase activity (P < 0.01).
28231388	0	33	theme	Antifatigue	32:42	arg1	Effect					44:49	Antifatigue Effect	32:49	Antifatigue Effect	32:49	Structural Characterization and Antifatigue Effect In Vivo of Maca (Lepidium meyenii Walp) Polysaccharide.
28231388	8	34	theme	lactate	1267:1273	arg1	dehydrogenase					1275:1287	lactate dehydrogenase	1267:1287	decreased lactate dehydrogenase activity (P < 0.01)	1257:1307	Compared with the control model, high-dosage (100 mg/kg bw/d) MP treatment could significantly enhance glutathione peroxidase and creatine kinase activities (P < 0.05) and decreased lactate dehydrogenase activity (P < 0.01).
28231388	2	35	theme	physicochemical	276:290	arg1	properties					292:301	The physicochemical properties	272:301	The physicochemical properties of MP	272:307	The physicochemical properties of MP were analyzed through high-performance gel filtration chromatography, IR, monosaccharide composition, methylation, GC-MS, and NMR analyses.
28231388	9	36	theme	malondialdehyde	1404:1418	arg1	levels					1356:1361	the levels	1352:1361	the levels of blood urea nitrogen, lactic acid, and malondialdehyde (P < 0.05)	1352:1429	High-dosage MP could significantly reduce the levels of blood urea nitrogen, lactic acid, and malondialdehyde (P < 0.05).
28231388	10	37	theme	D-GalA	1475:1480	arg1	responsible					1506:1516	responsible	1506:1516	responsible	1506:1516	MP is an acidic polysaccharide with a high D-GalA content, which could be responsible for the antifatigue effect of maca.
28231388	10	37	theme	D-GalA	1475:1480	arg1	content					1482:1488	a high D-GalA content	1468:1488	a high D-GalA content	1468:1488	MP is an acidic polysaccharide with a high D-GalA content, which could be responsible for the antifatigue effect of maca.
28231388	7	38	theme	average	964:970	arg1	speeds					981:986	average swimming speeds	964:986	average swimming speeds (within the 1st 5 min) of mice (P < 0.05)	964:1028	MP (above mid-dosage 50 mg/kg bw/d) could effectively elongate swimming durations and accelerate average swimming speeds (within the 1st 5 min) of mice (P < 0.05) and improve the serous biochemical parameters of mice.
28231388	8	39	theme	kinase	1224:1229	arg1	<					1245:1245	P < 0.05	1243:1250	P < 0.05	1243:1250	Compared with the control model, high-dosage (100 mg/kg bw/d) MP treatment could significantly enhance glutathione peroxidase and creatine kinase activities (P < 0.05) and decreased lactate dehydrogenase activity (P < 0.01).
28231388	8	39	theme	kinase	1224:1229	arg1	activities					1231:1240	glutathione peroxidase and creatine kinase activities	1188:1240	glutathione peroxidase and creatine kinase activities (P < 0.05)	1188:1251	Compared with the control model, high-dosage (100 mg/kg bw/d) MP treatment could significantly enhance glutathione peroxidase and creatine kinase activities (P < 0.05) and decreased lactate dehydrogenase activity (P < 0.01).
28231388	3	40	theme	antifatigue	453:463	arg1	effect					465:470	The antifatigue effect	449:470	The antifatigue effect of MP	449:476	The antifatigue effect of MP was evaluated by using a mouse weight-loaded swimming model.
28231388	5	41	theme	mol	726:728	arg1	%					731:731	mol, %	726:731	%	731:731	It is composed of D-GalA: D-Glc: L-Ara: D-Man: D-Gal: L-Rha = 35.07:29.98:16.98:13.01:4.21:0.75 (mol, %).
28231388	5	41	theme	mol	726:728	arg1	35.07:29.98:16.98:13.01:4.21:0.75					691:723	35.07:29.98:16.98:13.01:4.21:0.75	691:723	35.07:29.98:16.98:13.01:4.21:0.75	691:723	It is composed of D-GalA: D-Glc: L-Ara: D-Man: D-Gal: L-Rha = 35.07:29.98:16.98:13.01:4.21:0.75 (mol, %).
28231388	9	42	theme	lactic	1387:1392	arg1	acid					1394:1397	lactic acid	1387:1397	lactic acid	1387:1397	High-dosage MP could significantly reduce the levels of blood urea nitrogen, lactic acid, and malondialdehyde (P < 0.05).
28231388	8	43	theme	dehydrogenase	1275:1287	arg1	activity					1289:1296	decreased lactate dehydrogenase activity	1257:1296	decreased lactate dehydrogenase activity (P < 0.01)	1257:1307	Compared with the control model, high-dosage (100 mg/kg bw/d) MP treatment could significantly enhance glutathione peroxidase and creatine kinase activities (P < 0.05) and decreased lactate dehydrogenase activity (P < 0.01).
28231388	8	43	theme	dehydrogenase	1275:1287	arg1	<					1301:1301	P < 0.01	1299:1306	P < 0.01	1299:1306	Compared with the control model, high-dosage (100 mg/kg bw/d) MP treatment could significantly enhance glutathione peroxidase and creatine kinase activities (P < 0.05) and decreased lactate dehydrogenase activity (P < 0.01).
28231388	8	44	theme	P	1243:1243	arg1	<					1245:1245	P < 0.05	1243:1250	P < 0.05	1243:1250	Compared with the control model, high-dosage (100 mg/kg bw/d) MP treatment could significantly enhance glutathione peroxidase and creatine kinase activities (P < 0.05) and decreased lactate dehydrogenase activity (P < 0.01).
28231388	8	44	theme	P	1243:1243	arg1	activities					1231:1240	glutathione peroxidase and creatine kinase activities	1188:1240	glutathione peroxidase and creatine kinase activities (P < 0.05)	1188:1251	Compared with the control model, high-dosage (100 mg/kg bw/d) MP treatment could significantly enhance glutathione peroxidase and creatine kinase activities (P < 0.05) and decreased lactate dehydrogenase activity (P < 0.01).
28231388	1	45	theme	%	176:176	arg1	purity					162:167	purity	162:167	purity of 99.2%	162:176	Maca (Lepidium meyenii Walp) polysaccharides (MP) with purity of 99.2% were obtained to investigate their structural characteristics and antifatigue effect in vivo.
28231388	8	46	theme	P	1299:1299	arg1	activity					1289:1296	decreased lactate dehydrogenase activity	1257:1296	decreased lactate dehydrogenase activity (P < 0.01)	1257:1307	Compared with the control model, high-dosage (100 mg/kg bw/d) MP treatment could significantly enhance glutathione peroxidase and creatine kinase activities (P < 0.05) and decreased lactate dehydrogenase activity (P < 0.01).
28231388	8	46	theme	P	1299:1299	arg1	<					1301:1301	P < 0.01	1299:1306	P < 0.01	1299:1306	Compared with the control model, high-dosage (100 mg/kg bw/d) MP treatment could significantly enhance glutathione peroxidase and creatine kinase activities (P < 0.05) and decreased lactate dehydrogenase activity (P < 0.01).
28231388	7	47	theme	P	1020:1020	arg1	mice					1014:1017	mice	1014:1017	mice (P < 0.05)	1014:1028	MP (above mid-dosage 50 mg/kg bw/d) could effectively elongate swimming durations and accelerate average swimming speeds (within the 1st 5 min) of mice (P < 0.05) and improve the serous biochemical parameters of mice.
28231388	7	47	theme	P	1020:1020	arg1	<					1022:1022	P < 0.05	1020:1027	P < 0.05	1020:1027	MP (above mid-dosage 50 mg/kg bw/d) could effectively elongate swimming durations and accelerate average swimming speeds (within the 1st 5 min) of mice (P < 0.05) and improve the serous biochemical parameters of mice.
28231388	7	48	theme	mice	1079:1082	arg1	parameters					1065:1074	the serous biochemical parameters	1042:1074	the serous biochemical parameters of mice	1042:1082	MP (above mid-dosage 50 mg/kg bw/d) could effectively elongate swimming durations and accelerate average swimming speeds (within the 1st 5 min) of mice (P < 0.05) and improve the serous biochemical parameters of mice.
28231388	9	49	theme	P	1421:1421	arg1	malondialdehyde					1404:1418	malondialdehyde	1404:1418	malondialdehyde (P < 0.05)	1404:1429	High-dosage MP could significantly reduce the levels of blood urea nitrogen, lactic acid, and malondialdehyde (P < 0.05).
28231388	9	49	theme	P	1421:1421	arg1	<					1423:1423	P < 0.05	1421:1428	P < 0.05	1421:1428	High-dosage MP could significantly reduce the levels of blood urea nitrogen, lactic acid, and malondialdehyde (P < 0.05).
28231388	3	50	theme	MP	475:476	arg1	effect					465:470	The antifatigue effect	449:470	The antifatigue effect of MP	449:476	The antifatigue effect of MP was evaluated by using a mouse weight-loaded swimming model.
28231388	7	51	theme	swimming	930:937	arg1	durations					939:947	swimming durations	930:947	swimming durations	930:947	MP (above mid-dosage 50 mg/kg bw/d) could effectively elongate swimming durations and accelerate average swimming speeds (within the 1st 5 min) of mice (P < 0.05) and improve the serous biochemical parameters of mice.
28231388	9	52	theme	blood	1366:1370	arg1	nitrogen					1377:1384	blood urea nitrogen	1366:1384	blood urea nitrogen	1366:1384	High-dosage MP could significantly reduce the levels of blood urea nitrogen, lactic acid, and malondialdehyde (P < 0.05).
28231388	8	53	theme	high-dosage	1118:1128	arg1	treatment					1150:1158	high-dosage (100 mg/kg bw/d) MP treatment	1118:1158	high-dosage (100 mg/kg bw/d) MP treatment	1118:1158	Compared with the control model, high-dosage (100 mg/kg bw/d) MP treatment could significantly enhance glutathione peroxidase and creatine kinase activities (P < 0.05) and decreased lactate dehydrogenase activity (P < 0.01).
28231388	10	54	theme	maca	1548:1551	arg1	effect					1538:1543	the antifatigue effect	1522:1543	the antifatigue effect of maca	1522:1551	MP is an acidic polysaccharide with a high D-GalA content, which could be responsible for the antifatigue effect of maca.
28231388	7	55	theme	mg/kg	891:895	arg1	MP					867:868	MP	867:868	MP (above mid-dosage 50 mg/kg bw/d)	867:901	MP (above mid-dosage 50 mg/kg bw/d) could effectively elongate swimming durations and accelerate average swimming speeds (within the 1st 5 min) of mice (P < 0.05) and improve the serous biochemical parameters of mice.
28231388	7	55	theme	mg/kg	891:895	arg1	bw/d					897:900	above mid-dosage 50 mg/kg bw/d	871:900	above mid-dosage 50 mg/kg bw/d	871:900	MP (above mid-dosage 50 mg/kg bw/d) could effectively elongate swimming durations and accelerate average swimming speeds (within the 1st 5 min) of mice (P < 0.05) and improve the serous biochemical parameters of mice.
28231388	1	56	theme	Lepidium	113:120	arg1	Maca					107:110	Maca	107:110	Maca (Lepidium meyenii Walp) polysaccharides (MP) with purity of 99.2%	107:176	Maca (Lepidium meyenii Walp) polysaccharides (MP) with purity of 99.2% were obtained to investigate their structural characteristics and antifatigue effect in vivo.
28231388	1	56	theme	Lepidium	113:120	arg1	Walp					130:133	Lepidium meyenii Walp	113:133	Lepidium meyenii Walp	113:133	Maca (Lepidium meyenii Walp) polysaccharides (MP) with purity of 99.2% were obtained to investigate their structural characteristics and antifatigue effect in vivo.
28231388	4	57	theme	molecular	592:600	arg1	Mw					610:611	Mw	610:611	Mw	610:611	MP is an acidic heteropolysaccharide with an average molecular weight (Mw ) of 793.5 kDa.
28231388	4	57	theme	molecular	592:600	arg1	weight					602:607	an average molecular weight	581:607	an average molecular weight (Mw ) of 793.5 kDa	581:626	MP is an acidic heteropolysaccharide with an average molecular weight (Mw ) of 793.5 kDa.
28231388	8	58	theme	glutathione	1188:1198	arg1	peroxidase					1200:1209	glutathione peroxidase	1188:1209	glutathione peroxidase	1188:1209	Compared with the control model, high-dosage (100 mg/kg bw/d) MP treatment could significantly enhance glutathione peroxidase and creatine kinase activities (P < 0.05) and decreased lactate dehydrogenase activity (P < 0.01).
28231388	8	59	dep	high-dosage	1118:1128	arg1	mg/kg					1135:1139	100 mg/kg bw/d	1131:1144	100 mg/kg bw/d	1131:1144	Compared with the control model, high-dosage (100 mg/kg bw/d) MP treatment could significantly enhance glutathione peroxidase and creatine kinase activities (P < 0.05) and decreased lactate dehydrogenase activity (P < 0.01).
28231388	7	60	theme	above	871:875	arg1	MP					867:868	MP	867:868	MP (above mid-dosage 50 mg/kg bw/d)	867:901	MP (above mid-dosage 50 mg/kg bw/d) could effectively elongate swimming durations and accelerate average swimming speeds (within the 1st 5 min) of mice (P < 0.05) and improve the serous biochemical parameters of mice.
28231388	7	60	theme	above	871:875	arg1	bw/d					897:900	above mid-dosage 50 mg/kg bw/d	871:900	above mid-dosage 50 mg/kg bw/d	871:900	MP (above mid-dosage 50 mg/kg bw/d) could effectively elongate swimming durations and accelerate average swimming speeds (within the 1st 5 min) of mice (P < 0.05) and improve the serous biochemical parameters of mice.
28231388	1	61	theme	meyenii	122:128	arg1	Maca					107:110	Maca	107:110	Maca (Lepidium meyenii Walp) polysaccharides (MP) with purity of 99.2%	107:176	Maca (Lepidium meyenii Walp) polysaccharides (MP) with purity of 99.2% were obtained to investigate their structural characteristics and antifatigue effect in vivo.
28231388	1	61	theme	meyenii	122:128	arg1	Walp					130:133	Lepidium meyenii Walp	113:133	Lepidium meyenii Walp	113:133	Maca (Lepidium meyenii Walp) polysaccharides (MP) with purity of 99.2% were obtained to investigate their structural characteristics and antifatigue effect in vivo.
28231388	4	62	theme	average	584:590	arg1	Mw					610:611	Mw	610:611	Mw	610:611	MP is an acidic heteropolysaccharide with an average molecular weight (Mw ) of 793.5 kDa.
28231388	4	62	theme	average	584:590	arg1	weight					602:607	an average molecular weight	581:607	an average molecular weight (Mw ) of 793.5 kDa	581:626	MP is an acidic heteropolysaccharide with an average molecular weight (Mw ) of 793.5 kDa.
28231388	4	63	with	heteropolysaccharide	555:574	arg1	Mw					610:611	Mw	610:611	Mw	610:611	MP is an acidic heteropolysaccharide with an average molecular weight (Mw ) of 793.5 kDa.
28231388	4	63	with	heteropolysaccharide	555:574	arg1	weight					602:607	an average molecular weight	581:607	an average molecular weight (Mw ) of 793.5 kDa	581:626	MP is an acidic heteropolysaccharide with an average molecular weight (Mw ) of 793.5 kDa.
28231388	7	64	theme	mid-dosage	877:886	arg1	MP					867:868	MP	867:868	MP (above mid-dosage 50 mg/kg bw/d)	867:901	MP (above mid-dosage 50 mg/kg bw/d) could effectively elongate swimming durations and accelerate average swimming speeds (within the 1st 5 min) of mice (P < 0.05) and improve the serous biochemical parameters of mice.
28231388	7	64	theme	mid-dosage	877:886	arg1	bw/d					897:900	above mid-dosage 50 mg/kg bw/d	871:900	above mid-dosage 50 mg/kg bw/d	871:900	MP (above mid-dosage 50 mg/kg bw/d) could effectively elongate swimming durations and accelerate average swimming speeds (within the 1st 5 min) of mice (P < 0.05) and improve the serous biochemical parameters of mice.
28231388	8	65	theme	MP	1147:1148	arg1	treatment					1150:1158	high-dosage (100 mg/kg bw/d) MP treatment	1118:1158	high-dosage (100 mg/kg bw/d) MP treatment	1118:1158	Compared with the control model, high-dosage (100 mg/kg bw/d) MP treatment could significantly enhance glutathione peroxidase and creatine kinase activities (P < 0.05) and decreased lactate dehydrogenase activity (P < 0.01).
28231388	10	66	theme	acidic	1441:1446	arg1	polysaccharide					1448:1461	an acidic polysaccharide	1438:1461	an acidic polysaccharide with a high D-GalA content, which could be responsible for the antifatigue effect of maca	1438:1551	MP is an acidic polysaccharide with a high D-GalA content, which could be responsible for the antifatigue effect of maca.
28231388	10	66	theme	acidic	1441:1446	arg1	MP					1432:1433	MP	1432:1433	MP	1432:1433	MP is an acidic polysaccharide with a high D-GalA content, which could be responsible for the antifatigue effect of maca.
28231388	9	67	theme	urea	1372:1375	arg1	nitrogen					1377:1384	blood urea nitrogen	1366:1384	blood urea nitrogen	1366:1384	High-dosage MP could significantly reduce the levels of blood urea nitrogen, lactic acid, and malondialdehyde (P < 0.05).
28231388	0	68	theme	Maca	62:65	arg1	Polysaccharide					91:104	Maca (Lepidium meyenii Walp) Polysaccharide	62:104	Maca (Lepidium meyenii Walp) Polysaccharide	62:104	Structural Characterization and Antifatigue Effect In Vivo of Maca (Lepidium meyenii Walp) Polysaccharide.
28231388	8	69	theme	decreased	1257:1265	arg1	activity					1289:1296	decreased lactate dehydrogenase activity	1257:1296	decreased lactate dehydrogenase activity (P < 0.01)	1257:1307	Compared with the control model, high-dosage (100 mg/kg bw/d) MP treatment could significantly enhance glutathione peroxidase and creatine kinase activities (P < 0.05) and decreased lactate dehydrogenase activity (P < 0.01).
28231388	8	69	theme	decreased	1257:1265	arg1	<					1301:1301	P < 0.01	1299:1306	P < 0.01	1299:1306	Compared with the control model, high-dosage (100 mg/kg bw/d) MP treatment could significantly enhance glutathione peroxidase and creatine kinase activities (P < 0.05) and decreased lactate dehydrogenase activity (P < 0.01).
28231388	2	70	theme	filtration	352:361	arg1	chromatography					363:376	high-performance gel filtration chromatography	331:376	high-performance gel filtration chromatography	331:376	The physicochemical properties of MP were analyzed through high-performance gel filtration chromatography, IR, monosaccharide composition, methylation, GC-MS, and NMR analyses.
26276259	6	0	theme	phosphate	976:984	arg1	buffer					986:991	phosphate buffer	976:991	phosphate buffer (pH 7.5) at 6, 12 and 24 h	976:1018	The releases (%) in phosphate buffer (pH 7.5) at 6, 12 and 24 h were selected as responses to depict the mode of release and similarity factor (f2), between release profiles in 0.1N HCl and pH 7.5 during the first 8 h, as response of pH-independence.
26276259	6	0	theme	phosphate	976:984	arg1	pH					994:995	pH 7.5	994:999	pH 7.5	994:999	The releases (%) in phosphate buffer (pH 7.5) at 6, 12 and 24 h were selected as responses to depict the mode of release and similarity factor (f2), between release profiles in 0.1N HCl and pH 7.5 during the first 8 h, as response of pH-independence.
26276259	7	1	theme	overall	1280:1286	arg1	values					1301:1306	overall desirability values	1280:1306	overall desirability values	1280:1306	A desirability function combining the four responses was constructed and overall desirability values were used for the ANN and MLR modeling.
26276259	9	2	theme	formulation	1679:1689	arg1	prediction					1665:1674	prediction	1665:1674	prediction of formulation with optimal in vitro drug release	1665:1724	The constructed ANN model fitted better to the overall desirability than the MLR model (R=0.838 vs. 0.670, for the additional checkpoint formulations) and therefore, was used for prediction of formulation with optimal in vitro drug release.
26276259	3	3	from	former	585:590	arg1	VH					535:536	VH	535:536	VH in intermediate layer, X1, and of XG in matrix former of intermediate and outer layers, X2 and X3	535:634	A face-centered central composite experimental design was employed with three factors (mass fraction of VH in intermediate layer, X1, and of XG in matrix former of intermediate and outer layers, X2 and X3).
26276259	1	4	theme	chronotherapeutic	249:265	arg1	release					282:288	chronotherapeutic pH-independent release	249:288	chronotherapeutic pH-independent release of verapamil HCl (VH)	249:310	The aim of this work was to evaluate and optimize formulation of three-layer matrix tablets based on xanthan gum (XG) and sodium alginate for chronotherapeutic pH-independent release of verapamil HCl (VH).
26276259	4	5	dep	in	675:676	arg1	vitro					678:682	vitro	678:682	vitro	678:682	The prepared tablets were tested for in vitro release in 0.1 N HCl and phosphate buffer (pH 7.5), tensile strength and friability.
26276259	7	6	theme	desirability	1209:1220	arg1	function					1222:1229	A desirability function	1207:1229	A desirability function combining the four responses	1207:1258	A desirability function combining the four responses was constructed and overall desirability values were used for the ANN and MLR modeling.
26276259	9	7	theme	optimal	1696:1702	arg1	release					1718:1724	optimal in vitro drug release	1696:1724	optimal in vitro drug release	1696:1724	The constructed ANN model fitted better to the overall desirability than the MLR model (R=0.838 vs. 0.670, for the additional checkpoint formulations) and therefore, was used for prediction of formulation with optimal in vitro drug release.
26276259	5	8	dep	release	924:930	arg1	behavior					932:939	behavior	932:939	behavior	932:939	Furthermore, swelling observation and release modeling to Weibull function and power law equation of Peppas were employed to help further understanding of release behavior and mechanism.
26276259	8	9	used	used	1426:1429	arg2	formulations					1375:1386	Five additional checkpoint formulations	1348:1386	Five additional checkpoint formulations	1348:1386	Five additional checkpoint formulations, within the experimental domain, were used to validate the external predictability of the models.
26276259	6	10	theme	factor	1092:1097	arg1	mode					1061:1064	the mode	1057:1064	the mode of release and similarity factor (f2)	1057:1102	The releases (%) in phosphate buffer (pH 7.5) at 6, 12 and 24 h were selected as responses to depict the mode of release and similarity factor (f2), between release profiles in 0.1N HCl and pH 7.5 during the first 8 h, as response of pH-independence.
26276259	1	11	theme	three-layer	172:182	arg1	tablets					191:197	three-layer matrix tablets	172:197	three-layer matrix tablets based on xanthan gum (XG) and sodium alginate	172:243	The aim of this work was to evaluate and optimize formulation of three-layer matrix tablets based on xanthan gum (XG) and sodium alginate for chronotherapeutic pH-independent release of verapamil HCl (VH).
26276259	3	12	theme	intermediate	541:552	arg1	layer					554:558	intermediate layer	541:558	intermediate layer	541:558	A face-centered central composite experimental design was employed with three factors (mass fraction of VH in intermediate layer, X1, and of XG in matrix former of intermediate and outer layers, X2 and X3).
26276259	1	13	theme	tablets	191:197	arg1	formulation					157:167	formulation	157:167	formulation of three-layer matrix tablets based on xanthan gum (XG) and sodium alginate for chronotherapeutic pH-independent release of verapamil HCl (VH)	157:310	The aim of this work was to evaluate and optimize formulation of three-layer matrix tablets based on xanthan gum (XG) and sodium alginate for chronotherapeutic pH-independent release of verapamil HCl (VH).
26276259	9	14	theme	ANN	1502:1504	arg1	model					1506:1510	The constructed ANN model	1486:1510	The constructed ANN model	1486:1510	The constructed ANN model fitted better to the overall desirability than the MLR model (R=0.838 vs. 0.670, for the additional checkpoint formulations) and therefore, was used for prediction of formulation with optimal in vitro drug release.
26276259	9	15	dep	model	1567:1571	arg1	R=0.838					1574:1580	R=0.838	1574:1580	R=0.838	1574:1580	The constructed ANN model fitted better to the overall desirability than the MLR model (R=0.838 vs. 0.670, for the additional checkpoint formulations) and therefore, was used for prediction of formulation with optimal in vitro drug release.
26276259	9	15	dep	model	1567:1571	arg1	0.670					1586:1590	0.670	1586:1590	0.670	1586:1590	The constructed ANN model fitted better to the overall desirability than the MLR model (R=0.838 vs. 0.670, for the additional checkpoint formulations) and therefore, was used for prediction of formulation with optimal in vitro drug release.
26276259	3	16	theme	VH	535:536	arg1	fraction					523:530	mass fraction	518:530	mass fraction of VH in intermediate layer, X1, and of XG in matrix former of intermediate and outer layers, X2 and X3	518:634	A face-centered central composite experimental design was employed with three factors (mass fraction of VH in intermediate layer, X1, and of XG in matrix former of intermediate and outer layers, X2 and X3).
26276259	6	17	theme	release	1069:1075	arg1	mode					1061:1064	the mode	1057:1064	the mode of release and similarity factor (f2)	1057:1102	The releases (%) in phosphate buffer (pH 7.5) at 6, 12 and 24 h were selected as responses to depict the mode of release and similarity factor (f2), between release profiles in 0.1N HCl and pH 7.5 during the first 8 h, as response of pH-independence.
26276259	9	18	dep	in	1704:1705	arg1	vitro					1707:1711	vitro	1707:1711	vitro	1707:1711	The constructed ANN model fitted better to the overall desirability than the MLR model (R=0.838 vs. 0.670, for the additional checkpoint formulations) and therefore, was used for prediction of formulation with optimal in vitro drug release.
26276259	1	19	theme	work	123:126	arg1	aim					111:113	The aim	107:113	The aim of this work	107:126	The aim of this work was to evaluate and optimize formulation of three-layer matrix tablets based on xanthan gum (XG) and sodium alginate for chronotherapeutic pH-independent release of verapamil HCl (VH).
26276259	9	20	theme	overall	1533:1539	arg1	desirability					1541:1552	the overall desirability	1529:1552	the overall desirability than the MLR model (R=0.838 vs. 0.670, for the additional checkpoint formulations)	1529:1635	The constructed ANN model fitted better to the overall desirability than the MLR model (R=0.838 vs. 0.670, for the additional checkpoint formulations) and therefore, was used for prediction of formulation with optimal in vitro drug release.
26276259	3	21	from	VH	535:536	arg1	layer					554:558	intermediate layer	541:558	intermediate layer	541:558	A face-centered central composite experimental design was employed with three factors (mass fraction of VH in intermediate layer, X1, and of XG in matrix former of intermediate and outer layers, X2 and X3).
26276259	3	21	from	VH	535:536	arg1	former					585:590	former	585:590	former	585:590	A face-centered central composite experimental design was employed with three factors (mass fraction of VH in intermediate layer, X1, and of XG in matrix former of intermediate and outer layers, X2 and X3).
26276259	6	22	theme	release	1113:1119	arg1	profiles					1121:1128	release profiles	1113:1128	release profiles in 0.1N HCl and pH 7.5 during the first 8 h	1113:1172	The releases (%) in phosphate buffer (pH 7.5) at 6, 12 and 24 h were selected as responses to depict the mode of release and similarity factor (f2), between release profiles in 0.1N HCl and pH 7.5 during the first 8 h, as response of pH-independence.
26276259	5	23	theme	release	807:813	arg1	modeling					815:822	release modeling	807:822	release modeling	807:822	Furthermore, swelling observation and release modeling to Weibull function and power law equation of Peppas were employed to help further understanding of release behavior and mechanism.
26276259	5	24	theme	Weibull	827:833	arg1	function					835:842	Weibull function	827:842	Weibull function	827:842	Furthermore, swelling observation and release modeling to Weibull function and power law equation of Peppas were employed to help further understanding of release behavior and mechanism.
26276259	9	25	theme	additional	1601:1610	arg1	checkpoint					1612:1621	the additional checkpoint	1597:1621	the additional checkpoint	1597:1621	The constructed ANN model fitted better to the overall desirability than the MLR model (R=0.838 vs. 0.670, for the additional checkpoint formulations) and therefore, was used for prediction of formulation with optimal in vitro drug release.
26276259	5	26	theme	swelling	782:789	arg1	observation					791:801	swelling observation	782:801	swelling observation	782:801	Furthermore, swelling observation and release modeling to Weibull function and power law equation of Peppas were employed to help further understanding of release behavior and mechanism.
26276259	9	27	theme	MLR	1563:1565	arg1	model					1567:1571	the MLR model	1559:1571	the MLR model (R=0.838 vs. 0.670, for the additional checkpoint formulations)	1559:1635	The constructed ANN model fitted better to the overall desirability than the MLR model (R=0.838 vs. 0.670, for the additional checkpoint formulations) and therefore, was used for prediction of formulation with optimal in vitro drug release.
26276259	9	28	dep	R=0.838	1574:1580	arg1	formulations					1623:1634	formulations	1623:1634	formulations	1623:1634	The constructed ANN model fitted better to the overall desirability than the MLR model (R=0.838 vs. 0.670, for the additional checkpoint formulations) and therefore, was used for prediction of formulation with optimal in vitro drug release.
26276259	0	29	theme	matrix	91:96	arg1	tablets					98:104	three-layer matrix tablets	79:104	three-layer matrix tablets	79:104	Optimization of pH-independent chronotherapeutic release of verapamil HCl from three-layer matrix tablets.
26276259	4	30	from	friability	757:766	arg1	pH					727:728	pH 7.5	727:732	pH 7.5	727:732	The prepared tablets were tested for in vitro release in 0.1 N HCl and phosphate buffer (pH 7.5), tensile strength and friability.
26276259	4	30	from	friability	757:766	arg1	HCl					701:703	0.1 N HCl	695:703	0.1 N HCl	695:703	The prepared tablets were tested for in vitro release in 0.1 N HCl and phosphate buffer (pH 7.5), tensile strength and friability.
26276259	4	30	from	friability	757:766	arg1	buffer					719:724	phosphate buffer	709:724	phosphate buffer (pH 7.5)	709:733	The prepared tablets were tested for in vitro release in 0.1 N HCl and phosphate buffer (pH 7.5), tensile strength and friability.
26276259	0	31	from	Optimization	0:11	arg1	tablets					98:104	three-layer matrix tablets	79:104	three-layer matrix tablets	79:104	Optimization of pH-independent chronotherapeutic release of verapamil HCl from three-layer matrix tablets.
26276259	3	32	from	fraction	523:530	arg1	layer					554:558	intermediate layer	541:558	intermediate layer	541:558	A face-centered central composite experimental design was employed with three factors (mass fraction of VH in intermediate layer, X1, and of XG in matrix former of intermediate and outer layers, X2 and X3).
26276259	6	33	theme	first	1164:1168	arg1	h					1172:1172	the first 8 h	1160:1172	the first 8 h	1160:1172	The releases (%) in phosphate buffer (pH 7.5) at 6, 12 and 24 h were selected as responses to depict the mode of release and similarity factor (f2), between release profiles in 0.1N HCl and pH 7.5 during the first 8 h, as response of pH-independence.
26276259	4	34	from	strength	744:751	arg1	pH					727:728	pH 7.5	727:732	pH 7.5	727:732	The prepared tablets were tested for in vitro release in 0.1 N HCl and phosphate buffer (pH 7.5), tensile strength and friability.
26276259	4	34	from	strength	744:751	arg1	HCl					701:703	0.1 N HCl	695:703	0.1 N HCl	695:703	The prepared tablets were tested for in vitro release in 0.1 N HCl and phosphate buffer (pH 7.5), tensile strength and friability.
26276259	4	34	from	strength	744:751	arg1	buffer					719:724	phosphate buffer	709:724	phosphate buffer (pH 7.5)	709:733	The prepared tablets were tested for in vitro release in 0.1 N HCl and phosphate buffer (pH 7.5), tensile strength and friability.
26276259	4	35	from	release	684:690	arg1	pH					727:728	pH 7.5	727:732	pH 7.5	727:732	The prepared tablets were tested for in vitro release in 0.1 N HCl and phosphate buffer (pH 7.5), tensile strength and friability.
26276259	4	35	from	release	684:690	arg1	HCl					701:703	0.1 N HCl	695:703	0.1 N HCl	695:703	The prepared tablets were tested for in vitro release in 0.1 N HCl and phosphate buffer (pH 7.5), tensile strength and friability.
26276259	4	35	from	release	684:690	arg1	buffer					719:724	phosphate buffer	709:724	phosphate buffer (pH 7.5)	709:733	The prepared tablets were tested for in vitro release in 0.1 N HCl and phosphate buffer (pH 7.5), tensile strength and friability.
26276259	6	36	dep	The	956:958	arg1	releases					960:967	releases	960:967	releases	960:967	The releases (%) in phosphate buffer (pH 7.5) at 6, 12 and 24 h were selected as responses to depict the mode of release and similarity factor (f2), between release profiles in 0.1N HCl and pH 7.5 during the first 8 h, as response of pH-independence.
26276259	1	37	theme	pH-independent	267:280	arg1	release					282:288	chronotherapeutic pH-independent release	249:288	chronotherapeutic pH-independent release of verapamil HCl (VH)	249:310	The aim of this work was to evaluate and optimize formulation of three-layer matrix tablets based on xanthan gum (XG) and sodium alginate for chronotherapeutic pH-independent release of verapamil HCl (VH).
26276259	4	38	theme	in	675:676	arg1	release					684:690	in vitro release	675:690	in vitro release in 0.1 N HCl and phosphate buffer (pH 7.5)	675:733	The prepared tablets were tested for in vitro release in 0.1 N HCl and phosphate buffer (pH 7.5), tensile strength and friability.
26276259	2	39	theme	multiple	397:404	arg1	MLR					425:427	MLR	425:427	MLR	425:427	Artificial neural networks (ANN) were applied in the optimization and compared with multiple linear regression (MLR).
26276259	2	39	theme	multiple	397:404	arg1	regression					413:422	multiple linear regression	397:422	multiple linear regression (MLR)	397:428	Artificial neural networks (ANN) were applied in the optimization and compared with multiple linear regression (MLR).
26276259	0	40	theme	pH-independent	16:29	arg1	release					49:55	pH-independent chronotherapeutic release	16:55	pH-independent chronotherapeutic release of verapamil HCl from three-layer matrix tablets	16:104	Optimization of pH-independent chronotherapeutic release of verapamil HCl from three-layer matrix tablets.
26276259	7	41	theme	MLR	1334:1336	arg1	modeling					1338:1345	MLR modeling	1334:1345	MLR modeling	1334:1345	A desirability function combining the four responses was constructed and overall desirability values were used for the ANN and MLR modeling.
26276259	5	42	theme	law	854:856	arg1	equation					858:865	power law equation	848:865	power law equation of Peppas	848:875	Furthermore, swelling observation and release modeling to Weibull function and power law equation of Peppas were employed to help further understanding of release behavior and mechanism.
26276259	8	43	theme	additional	1353:1362	arg1	formulations					1375:1386	Five additional checkpoint formulations	1348:1386	Five additional checkpoint formulations	1348:1386	Five additional checkpoint formulations, within the experimental domain, were used to validate the external predictability of the models.
26276259	3	44	theme	intermediate	595:606	arg1	layers					618:623	intermediate and outer layers	595:623	intermediate and outer layers	595:623	A face-centered central composite experimental design was employed with three factors (mass fraction of VH in intermediate layer, X1, and of XG in matrix former of intermediate and outer layers, X2 and X3).
26276259	0	45	theme	release	49:55	arg1	Optimization					0:11	Optimization	0:11	Optimization of pH-independent chronotherapeutic release of verapamil HCl from three-layer matrix tablets.	0:105	Optimization of pH-independent chronotherapeutic release of verapamil HCl from three-layer matrix tablets.
26276259	1	46	theme	HCl	303:305	arg1	release					282:288	chronotherapeutic pH-independent release	249:288	chronotherapeutic pH-independent release of verapamil HCl (VH)	249:310	The aim of this work was to evaluate and optimize formulation of three-layer matrix tablets based on xanthan gum (XG) and sodium alginate for chronotherapeutic pH-independent release of verapamil HCl (VH).
26276259	6	47	from	profiles	1121:1128	arg1	pH					1146:1147	pH 7.5	1146:1151	pH 7.5	1146:1151	The releases (%) in phosphate buffer (pH 7.5) at 6, 12 and 24 h were selected as responses to depict the mode of release and similarity factor (f2), between release profiles in 0.1N HCl and pH 7.5 during the first 8 h, as response of pH-independence.
26276259	6	47	from	profiles	1121:1128	arg1	HCl					1138:1140	0.1N HCl	1133:1140	0.1N HCl	1133:1140	The releases (%) in phosphate buffer (pH 7.5) at 6, 12 and 24 h were selected as responses to depict the mode of release and similarity factor (f2), between release profiles in 0.1N HCl and pH 7.5 during the first 8 h, as response of pH-independence.
26276259	3	48	theme	experimental	465:476	arg1	design					478:483	A face-centered central composite experimental design	431:483	A face-centered central composite experimental design	431:483	A face-centered central composite experimental design was employed with three factors (mass fraction of VH in intermediate layer, X1, and of XG in matrix former of intermediate and outer layers, X2 and X3).
26276259	0	49	theme	verapamil	60:68	arg1	HCl					70:72	verapamil HCl	60:72	verapamil HCl	60:72	Optimization of pH-independent chronotherapeutic release of verapamil HCl from three-layer matrix tablets.
26276259	2	50	theme	Artificial	313:322	arg1	ANN					341:343	ANN	341:343	ANN	341:343	Artificial neural networks (ANN) were applied in the optimization and compared with multiple linear regression (MLR).
26276259	2	50	theme	Artificial	313:322	arg1	networks					331:338	Artificial neural networks	313:338	Artificial neural networks (ANN)	313:344	Artificial neural networks (ANN) were applied in the optimization and compared with multiple linear regression (MLR).
26276259	8	51	theme	external	1447:1454	arg1	predictability					1456:1469	the external predictability	1443:1469	the external predictability of the models	1443:1483	Five additional checkpoint formulations, within the experimental domain, were used to validate the external predictability of the models.
26276259	7	52	dep	ANN	1326:1328	arg1	the					1322:1324	the	1322:1324	the	1322:1324	A desirability function combining the four responses was constructed and overall desirability values were used for the ANN and MLR modeling.
26276259	8	53	theme	experimental	1400:1411	arg1	domain					1413:1418	the experimental domain	1396:1418	the experimental domain	1396:1418	Five additional checkpoint formulations, within the experimental domain, were used to validate the external predictability of the models.
26276259	3	54	theme	outer	612:616	arg1	layers					618:623	intermediate and outer layers	595:623	intermediate and outer layers	595:623	A face-centered central composite experimental design was employed with three factors (mass fraction of VH in intermediate layer, X1, and of XG in matrix former of intermediate and outer layers, X2 and X3).
26276259	4	55	theme	phosphate	709:717	arg1	pH					727:728	pH 7.5	727:732	pH 7.5	727:732	The prepared tablets were tested for in vitro release in 0.1 N HCl and phosphate buffer (pH 7.5), tensile strength and friability.
26276259	4	55	theme	phosphate	709:717	arg1	buffer					719:724	phosphate buffer	709:724	phosphate buffer (pH 7.5)	709:733	The prepared tablets were tested for in vitro release in 0.1 N HCl and phosphate buffer (pH 7.5), tensile strength and friability.
26276259	6	56	theme	pH-independence	1190:1204	arg1	response					1178:1185	response	1178:1185	response of pH-independence	1178:1204	The releases (%) in phosphate buffer (pH 7.5) at 6, 12 and 24 h were selected as responses to depict the mode of release and similarity factor (f2), between release profiles in 0.1N HCl and pH 7.5 during the first 8 h, as response of pH-independence.
26276259	7	57	theme	desirability	1288:1299	arg1	values					1301:1306	overall desirability values	1280:1306	overall desirability values	1280:1306	A desirability function combining the four responses was constructed and overall desirability values were used for the ANN and MLR modeling.
26276259	5	58	theme	further	899:905	arg1	understanding					907:919	further understanding	899:919	further understanding of release behavior and mechanism	899:953	Furthermore, swelling observation and release modeling to Weibull function and power law equation of Peppas were employed to help further understanding of release behavior and mechanism.
26276259	9	59	with	formulation	1679:1689	arg1	release					1718:1724	optimal in vitro drug release	1696:1724	optimal in vitro drug release	1696:1724	The constructed ANN model fitted better to the overall desirability than the MLR model (R=0.838 vs. 0.670, for the additional checkpoint formulations) and therefore, was used for prediction of formulation with optimal in vitro drug release.
26276259	8	60	theme	models	1478:1483	arg1	predictability					1456:1469	the external predictability	1443:1469	the external predictability of the models	1443:1483	Five additional checkpoint formulations, within the experimental domain, were used to validate the external predictability of the models.
26276259	1	61	theme	sodium	229:234	arg1	alginate					236:243	sodium alginate	229:243	sodium alginate	229:243	The aim of this work was to evaluate and optimize formulation of three-layer matrix tablets based on xanthan gum (XG) and sodium alginate for chronotherapeutic pH-independent release of verapamil HCl (VH).
26276259	3	62	theme	mass	518:521	arg1	fraction					523:530	mass fraction	518:530	mass fraction of VH in intermediate layer, X1, and of XG in matrix former of intermediate and outer layers, X2 and X3	518:634	A face-centered central composite experimental design was employed with three factors (mass fraction of VH in intermediate layer, X1, and of XG in matrix former of intermediate and outer layers, X2 and X3).
26276259	9	63	theme	in	1704:1705	arg1	release					1718:1724	optimal in vitro drug release	1696:1724	optimal in vitro drug release	1696:1724	The constructed ANN model fitted better to the overall desirability than the MLR model (R=0.838 vs. 0.670, for the additional checkpoint formulations) and therefore, was used for prediction of formulation with optimal in vitro drug release.
26276259	6	64	theme	similarity	1081:1090	arg1	factor					1092:1097	similarity factor	1081:1097	similarity factor (f2)	1081:1102	The releases (%) in phosphate buffer (pH 7.5) at 6, 12 and 24 h were selected as responses to depict the mode of release and similarity factor (f2), between release profiles in 0.1N HCl and pH 7.5 during the first 8 h, as response of pH-independence.
26276259	6	64	theme	similarity	1081:1090	arg1	f2					1100:1101	f2	1100:1101	f2	1100:1101	The releases (%) in phosphate buffer (pH 7.5) at 6, 12 and 24 h were selected as responses to depict the mode of release and similarity factor (f2), between release profiles in 0.1N HCl and pH 7.5 during the first 8 h, as response of pH-independence.
26276259	1	65	theme	matrix	184:189	arg1	tablets					191:197	three-layer matrix tablets	172:197	three-layer matrix tablets based on xanthan gum (XG) and sodium alginate	172:243	The aim of this work was to evaluate and optimize formulation of three-layer matrix tablets based on xanthan gum (XG) and sodium alginate for chronotherapeutic pH-independent release of verapamil HCl (VH).
26276259	4	66	theme	tensile	736:742	arg1	strength					744:751	tensile strength	736:751	tensile strength	736:751	The prepared tablets were tested for in vitro release in 0.1 N HCl and phosphate buffer (pH 7.5), tensile strength and friability.
26276259	9	67	theme	drug	1713:1716	arg1	release					1718:1724	optimal in vitro drug release	1696:1724	optimal in vitro drug release	1696:1724	The constructed ANN model fitted better to the overall desirability than the MLR model (R=0.838 vs. 0.670, for the additional checkpoint formulations) and therefore, was used for prediction of formulation with optimal in vitro drug release.
26276259	0	68	from	tablets	98:104	arg1	Optimization					0:11	Optimization	0:11	Optimization of pH-independent chronotherapeutic release of verapamil HCl from three-layer matrix tablets.	0:105	Optimization of pH-independent chronotherapeutic release of verapamil HCl from three-layer matrix tablets.
26276259	0	68	from	tablets	98:104	arg1	release					49:55	pH-independent chronotherapeutic release	16:55	pH-independent chronotherapeutic release of verapamil HCl from three-layer matrix tablets	16:104	Optimization of pH-independent chronotherapeutic release of verapamil HCl from three-layer matrix tablets.
26276259	9	69	theme	constructed	1490:1500	arg1	model					1506:1510	The constructed ANN model	1486:1510	The constructed ANN model	1486:1510	The constructed ANN model fitted better to the overall desirability than the MLR model (R=0.838 vs. 0.670, for the additional checkpoint formulations) and therefore, was used for prediction of formulation with optimal in vitro drug release.
26276259	1	70	theme	xanthan	208:214	arg1	XG					221:222	XG	221:222	XG	221:222	The aim of this work was to evaluate and optimize formulation of three-layer matrix tablets based on xanthan gum (XG) and sodium alginate for chronotherapeutic pH-independent release of verapamil HCl (VH).
26276259	1	70	theme	xanthan	208:214	arg1	gum					216:218	xanthan gum	208:218	xanthan gum (XG)	208:223	The aim of this work was to evaluate and optimize formulation of three-layer matrix tablets based on xanthan gum (XG) and sodium alginate for chronotherapeutic pH-independent release of verapamil HCl (VH).
26276259	6	71	theme	0.1N	1133:1136	arg1	HCl					1138:1140	0.1N HCl	1133:1140	0.1N HCl	1133:1140	The releases (%) in phosphate buffer (pH 7.5) at 6, 12 and 24 h were selected as responses to depict the mode of release and similarity factor (f2), between release profiles in 0.1N HCl and pH 7.5 during the first 8 h, as response of pH-independence.
26276259	3	72	dep	factors	509:515	arg1	fraction					523:530	mass fraction	518:530	mass fraction of VH in intermediate layer, X1, and of XG in matrix former of intermediate and outer layers, X2 and X3	518:634	A face-centered central composite experimental design was employed with three factors (mass fraction of VH in intermediate layer, X1, and of XG in matrix former of intermediate and outer layers, X2 and X3).
26276259	5	73	theme	mechanism	945:953	arg1	understanding					907:919	further understanding	899:919	further understanding of release behavior and mechanism	899:953	Furthermore, swelling observation and release modeling to Weibull function and power law equation of Peppas were employed to help further understanding of release behavior and mechanism.
26276259	3	74	theme	XG	572:573	arg1	VH					535:536	VH	535:536	VH in intermediate layer, X1, and of XG in matrix former of intermediate and outer layers, X2 and X3	535:634	A face-centered central composite experimental design was employed with three factors (mass fraction of VH in intermediate layer, X1, and of XG in matrix former of intermediate and outer layers, X2 and X3).
26276259	4	75	theme	prepared	642:649	arg1	tablets					651:657	The prepared tablets	638:657	The prepared tablets	638:657	The prepared tablets were tested for in vitro release in 0.1 N HCl and phosphate buffer (pH 7.5), tensile strength and friability.
26276259	3	76	theme	central	447:453	arg1	design					478:483	A face-centered central composite experimental design	431:483	A face-centered central composite experimental design	431:483	A face-centered central composite experimental design was employed with three factors (mass fraction of VH in intermediate layer, X1, and of XG in matrix former of intermediate and outer layers, X2 and X3).
26276259	0	77	theme	three-layer	79:89	arg1	tablets					98:104	three-layer matrix tablets	79:104	three-layer matrix tablets	79:104	Optimization of pH-independent chronotherapeutic release of verapamil HCl from three-layer matrix tablets.
26276259	6	78	from	h	1018:1018	arg1	buffer					986:991	phosphate buffer	976:991	phosphate buffer (pH 7.5) at 6, 12 and 24 h	976:1018	The releases (%) in phosphate buffer (pH 7.5) at 6, 12 and 24 h were selected as responses to depict the mode of release and similarity factor (f2), between release profiles in 0.1N HCl and pH 7.5 during the first 8 h, as response of pH-independence.
26276259	6	78	from	h	1018:1018	arg1	pH					994:995	pH 7.5	994:999	pH 7.5	994:999	The releases (%) in phosphate buffer (pH 7.5) at 6, 12 and 24 h were selected as responses to depict the mode of release and similarity factor (f2), between release profiles in 0.1N HCl and pH 7.5 during the first 8 h, as response of pH-independence.
26276259	5	79	theme	Peppas	870:875	arg1	observation					791:801	swelling observation	782:801	swelling observation	782:801	Furthermore, swelling observation and release modeling to Weibull function and power law equation of Peppas were employed to help further understanding of release behavior and mechanism.
26276259	5	79	theme	Peppas	870:875	arg1	modeling					815:822	release modeling	807:822	release modeling	807:822	Furthermore, swelling observation and release modeling to Weibull function and power law equation of Peppas were employed to help further understanding of release behavior and mechanism.
26276259	5	79	theme	Peppas	870:875	arg1	equation					858:865	power law equation	848:865	power law equation of Peppas	848:875	Furthermore, swelling observation and release modeling to Weibull function and power law equation of Peppas were employed to help further understanding of release behavior and mechanism.
26276259	3	80	from	XG	572:573	arg1	former					585:590	former	585:590	former	585:590	A face-centered central composite experimental design was employed with three factors (mass fraction of VH in intermediate layer, X1, and of XG in matrix former of intermediate and outer layers, X2 and X3).
26276259	3	81	from	layer	554:558	arg1	fraction					523:530	mass fraction	518:530	mass fraction of VH in intermediate layer, X1, and of XG in matrix former of intermediate and outer layers, X2 and X3	518:634	A face-centered central composite experimental design was employed with three factors (mass fraction of VH in intermediate layer, X1, and of XG in matrix former of intermediate and outer layers, X2 and X3).
26276259	0	82	theme	chronotherapeutic	31:47	arg1	release					49:55	pH-independent chronotherapeutic release	16:55	pH-independent chronotherapeutic release of verapamil HCl from three-layer matrix tablets	16:104	Optimization of pH-independent chronotherapeutic release of verapamil HCl from three-layer matrix tablets.
26276259	5	83	theme	power	848:852	arg1	equation					858:865	power law equation	848:865	power law equation of Peppas	848:875	Furthermore, swelling observation and release modeling to Weibull function and power law equation of Peppas were employed to help further understanding of release behavior and mechanism.
26276259	2	84	theme	linear	406:411	arg1	MLR					425:427	MLR	425:427	MLR	425:427	Artificial neural networks (ANN) were applied in the optimization and compared with multiple linear regression (MLR).
26276259	2	84	theme	linear	406:411	arg1	regression					413:422	multiple linear regression	397:422	multiple linear regression (MLR)	397:428	Artificial neural networks (ANN) were applied in the optimization and compared with multiple linear regression (MLR).
26276259	8	85	theme	checkpoint	1364:1373	arg1	formulations					1375:1386	Five additional checkpoint formulations	1348:1386	Five additional checkpoint formulations	1348:1386	Five additional checkpoint formulations, within the experimental domain, were used to validate the external predictability of the models.
26276259	3	86	theme	face-centered	433:445	arg1	design					478:483	A face-centered central composite experimental design	431:483	A face-centered central composite experimental design	431:483	A face-centered central composite experimental design was employed with three factors (mass fraction of VH in intermediate layer, X1, and of XG in matrix former of intermediate and outer layers, X2 and X3).
26276259	1	87	theme	verapamil	293:301	arg1	VH					308:309	VH	308:309	VH	308:309	The aim of this work was to evaluate and optimize formulation of three-layer matrix tablets based on xanthan gum (XG) and sodium alginate for chronotherapeutic pH-independent release of verapamil HCl (VH).
26276259	1	87	theme	verapamil	293:301	arg1	HCl					303:305	verapamil HCl	293:305	verapamil HCl (VH)	293:310	The aim of this work was to evaluate and optimize formulation of three-layer matrix tablets based on xanthan gum (XG) and sodium alginate for chronotherapeutic pH-independent release of verapamil HCl (VH).
26276259	7	88	used	used	1313:1316	arg2	values					1301:1306	overall desirability values	1280:1306	overall desirability values	1280:1306	A desirability function combining the four responses was constructed and overall desirability values were used for the ANN and MLR modeling.
26276259	0	89	theme	HCl	70:72	arg1	release					49:55	pH-independent chronotherapeutic release	16:55	pH-independent chronotherapeutic release of verapamil HCl from three-layer matrix tablets	16:104	Optimization of pH-independent chronotherapeutic release of verapamil HCl from three-layer matrix tablets.
26276259	5	90	theme	release	924:930	arg1	understanding					907:919	further understanding	899:919	further understanding of release behavior and mechanism	899:953	Furthermore, swelling observation and release modeling to Weibull function and power law equation of Peppas were employed to help further understanding of release behavior and mechanism.
26276259	3	91	theme	layers	618:623	arg1	former					585:590	former	585:590	former	585:590	A face-centered central composite experimental design was employed with three factors (mass fraction of VH in intermediate layer, X1, and of XG in matrix former of intermediate and outer layers, X2 and X3).
26276259	2	92	theme	neural	324:329	arg1	ANN					341:343	ANN	341:343	ANN	341:343	Artificial neural networks (ANN) were applied in the optimization and compared with multiple linear regression (MLR).
26276259	2	92	theme	neural	324:329	arg1	networks					331:338	Artificial neural networks	313:338	Artificial neural networks (ANN)	313:344	Artificial neural networks (ANN) were applied in the optimization and compared with multiple linear regression (MLR).
26276259	4	93	theme	N	699:699	arg1	HCl					701:703	0.1 N HCl	695:703	0.1 N HCl	695:703	The prepared tablets were tested for in vitro release in 0.1 N HCl and phosphate buffer (pH 7.5), tensile strength and friability.
26276259	3	94	theme	composite	455:463	arg1	design					478:483	A face-centered central composite experimental design	431:483	A face-centered central composite experimental design	431:483	A face-centered central composite experimental design was employed with three factors (mass fraction of VH in intermediate layer, X1, and of XG in matrix former of intermediate and outer layers, X2 and X3).
26276259	9	95	used	used	1656:1659	arg2	model					1506:1510	The constructed ANN model	1486:1510	The constructed ANN model	1486:1510	The constructed ANN model fitted better to the overall desirability than the MLR model (R=0.838 vs. 0.670, for the additional checkpoint formulations) and therefore, was used for prediction of formulation with optimal in vitro drug release.
25782009	8	0	theme	IL-2	1148:1151	arg1	secretion					1153:1161	IL-2 secretion	1148:1161	IL-2 secretion	1148:1161	TPPPS3 enhanced the secretion of cytokine IL-2 and TNF, whereas TPPPS2 mainly elevated IL-2 secretion.
25782009	4	1	dep	fractions	509:517	arg1	TPPPS2					528:533	TPPPS2	528:533	TPPPS2	528:533	Three homogeneous polysaccharide fractions (TPPPS1, TPPPS2, and TPPPS3) were purified from TPPPS by DEAE-cellulose column chromatography.
25782009	4	1	dep	fractions	509:517	arg1	TPPPS3					540:545	TPPPS3	540:545	TPPPS3	540:545	Three homogeneous polysaccharide fractions (TPPPS1, TPPPS2, and TPPPS3) were purified from TPPPS by DEAE-cellulose column chromatography.
25782009	4	1	dep	fractions	509:517	arg1	TPPPS1					520:525	TPPPS1	520:525	TPPPS1	520:525	Three homogeneous polysaccharide fractions (TPPPS1, TPPPS2, and TPPPS3) were purified from TPPPS by DEAE-cellulose column chromatography.
25782009	4	1	dep	fractions	509:517	arg1	fractions					509:517	Three homogeneous polysaccharide fractions	476:517	Three homogeneous polysaccharide fractions (TPPPS1, TPPPS2, and TPPPS3)	476:546	Three homogeneous polysaccharide fractions (TPPPS1, TPPPS2, and TPPPS3) were purified from TPPPS by DEAE-cellulose column chromatography.
25782009	6	2	theme	high-performance	724:739	arg1	chromatography					748:761	high-performance liquid chromatography	724:761	high-performance liquid chromatography (HPLC)	724:768	Results of high-performance liquid chromatography (HPLC) showed that TPPPS comprised mannose, ribose, xylose, glucuronic acid, galacturonic acid, glucose, galactose, and arabinose.
25782009	6	2	theme	high-performance	724:739	arg1	HPLC					764:767	HPLC	764:767	HPLC	764:767	Results of high-performance liquid chromatography (HPLC) showed that TPPPS comprised mannose, ribose, xylose, glucuronic acid, galacturonic acid, glucose, galactose, and arabinose.
25782009	1	3	theme	cellular	165:172	arg1	responses					193:201	cellular and humoral immune responses	165:201	cellular and humoral immune responses of animals	165:212	Taishan Pinus massoniana pollen polysaccharide (TPPPS) improves cellular and humoral immune responses of animals and is a novel potential immunomodulator.
25782009	0	4	theme	in	91:92	arg1	activity					32:39	biological activity	21:39	biological activity of Taishan Pinus massoniana pollen polysaccharide in vitro	21:98	Characterization and biological activity of Taishan Pinus massoniana pollen polysaccharide in vitro.
25782009	10	5	theme	adsorption	1445:1454	arg1	interference					1456:1467	virus adsorption interference	1439:1467	virus adsorption interference	1439:1467	TPPPS3 at 50 μg/mL significantly inhibited the proliferation of subgroup B Avian Leukosis virus (ALV-B) through virus adsorption interference in vitro.
25782009	5	6	theme	average	618:624	arg1	kDa					694:696	56, 25, and 128 kDa	678:696	56, 25, and 128 kDa	678:696	The average molecular weights of the three polysaccharides were 56, 25, and 128 kDa, respectively.
25782009	5	6	theme	average	618:624	arg1	weights					636:642	The average molecular weights	614:642	The average molecular weights of the three polysaccharides	614:671	The average molecular weights of the three polysaccharides were 56, 25, and 128 kDa, respectively.
25782009	4	7	theme	column	591:596	arg1	chromatography					598:611	DEAE-cellulose column chromatography	576:611	DEAE-cellulose column chromatography	576:611	Three homogeneous polysaccharide fractions (TPPPS1, TPPPS2, and TPPPS3) were purified from TPPPS by DEAE-cellulose column chromatography.
25782009	3	8	theme	crude	356:360	arg1	polysaccharide					362:375	crude polysaccharide	356:375	crude polysaccharide	356:375	To investigate the composition of TPPPS, crude polysaccharide was obtained from Taishan P. massoniana pollen through water extraction and ethanol precipitation.
25782009	11	9	theme	key	1629:1631	arg1	roles					1633:1637	key roles	1629:1637	key roles	1629:1637	Results indicated that TPPPS comprised three main components, among which, TPPPS1 mainly showed antioxidant effects, whereas TPPPS2 and TPPPS3 played key roles in immunomodulation, especially TPPPS3.
25782009	5	10	theme	molecular	626:634	arg1	kDa					694:696	56, 25, and 128 kDa	678:696	56, 25, and 128 kDa	678:696	The average molecular weights of the three polysaccharides were 56, 25, and 128 kDa, respectively.
25782009	5	10	theme	molecular	626:634	arg1	weights					636:642	The average molecular weights	614:642	The average molecular weights of the three polysaccharides	614:671	The average molecular weights of the three polysaccharides were 56, 25, and 128 kDa, respectively.
25782009	7	11	theme	lymphocyte	985:994	arg1	proliferation					996:1008	spleen lymphocyte proliferation	978:1008	spleen lymphocyte proliferation	978:1008	The biological activity assays showed that TPPPS2 and TPPPS3 significantly promoted spleen lymphocyte proliferation, and that TPPPS3 showed better effect than TPPPS2.
25782009	10	12	from	μg/mL	1340:1344	arg1	TPPPS3					1327:1332	TPPPS3	1327:1332	TPPPS3 at 50 μg/mL	1327:1344	TPPPS3 at 50 μg/mL significantly inhibited the proliferation of subgroup B Avian Leukosis virus (ALV-B) through virus adsorption interference in vitro.
25782009	11	13	theme	main	1524:1527	arg1	components					1529:1538	three main components	1518:1538	three main components	1518:1538	Results indicated that TPPPS comprised three main components, among which, TPPPS1 mainly showed antioxidant effects, whereas TPPPS2 and TPPPS3 played key roles in immunomodulation, especially TPPPS3.
25782009	7	14	theme	activity	909:916	arg1	assays					918:923	The biological activity assays	894:923	The biological activity assays	894:923	The biological activity assays showed that TPPPS2 and TPPPS3 significantly promoted spleen lymphocyte proliferation, and that TPPPS3 showed better effect than TPPPS2.
25782009	3	15	theme	TPPPS	349:353	arg1	composition					334:344	the composition	330:344	the composition of TPPPS	330:353	To investigate the composition of TPPPS, crude polysaccharide was obtained from Taishan P. massoniana pollen through water extraction and ethanol precipitation.
25782009	4	16	theme	polysaccharide	494:507	arg1	TPPPS2					528:533	TPPPS2	528:533	TPPPS2	528:533	Three homogeneous polysaccharide fractions (TPPPS1, TPPPS2, and TPPPS3) were purified from TPPPS by DEAE-cellulose column chromatography.
25782009	4	16	theme	polysaccharide	494:507	arg1	TPPPS3					540:545	TPPPS3	540:545	TPPPS3	540:545	Three homogeneous polysaccharide fractions (TPPPS1, TPPPS2, and TPPPS3) were purified from TPPPS by DEAE-cellulose column chromatography.
25782009	4	16	theme	polysaccharide	494:507	arg1	TPPPS1					520:525	TPPPS1	520:525	TPPPS1	520:525	Three homogeneous polysaccharide fractions (TPPPS1, TPPPS2, and TPPPS3) were purified from TPPPS by DEAE-cellulose column chromatography.
25782009	4	16	theme	polysaccharide	494:507	arg1	fractions					509:517	Three homogeneous polysaccharide fractions	476:517	Three homogeneous polysaccharide fractions (TPPPS1, TPPPS2, and TPPPS3)	476:546	Three homogeneous polysaccharide fractions (TPPPS1, TPPPS2, and TPPPS3) were purified from TPPPS by DEAE-cellulose column chromatography.
25782009	9	17	theme	other	1194:1198	arg1	effects					1200:1206	other effects	1194:1206	other effects	1194:1206	By contrast, TPPPS1 exhibited other effects, and it induced the highest amount of NO production, thereby indicating that TPPPS1 had the best antioxidant activity.
25782009	2	18	theme	TPPPS	283:287	arg1	components					269:278	the components	265:278	the components of TPPPS	265:287	However, the components of TPPPS have not been recognized.
25782009	1	19	theme	humoral	178:184	arg1	responses					193:201	cellular and humoral immune responses	165:201	cellular and humoral immune responses of animals	165:212	Taishan Pinus massoniana pollen polysaccharide (TPPPS) improves cellular and humoral immune responses of animals and is a novel potential immunomodulator.
25782009	9	20	theme	best	1300:1303	arg1	activity					1317:1324	the best antioxidant activity	1296:1324	the best antioxidant activity	1296:1324	By contrast, TPPPS1 exhibited other effects, and it induced the highest amount of NO production, thereby indicating that TPPPS1 had the best antioxidant activity.
25782009	12	21	theme	immunomodulator	1792:1806	arg1	development					1764:1774	the development	1760:1774	the development of an effective immunomodulator	1760:1806	Further studies on the use of a reasonable proportion of TPPPS1-3 may facilitate the development of an effective immunomodulator.
25782009	10	22	theme	virus	1417:1421	arg1	proliferation					1374:1386	the proliferation	1370:1386	the proliferation of subgroup B Avian Leukosis virus (ALV-B)	1370:1429	TPPPS3 at 50 μg/mL significantly inhibited the proliferation of subgroup B Avian Leukosis virus (ALV-B) through virus adsorption interference in vitro.
25782009	9	23	theme	highest	1228:1234	arg1	amount					1236:1241	the highest amount	1224:1241	the highest amount of NO production	1224:1258	By contrast, TPPPS1 exhibited other effects, and it induced the highest amount of NO production, thereby indicating that TPPPS1 had the best antioxidant activity.
25782009	9	23	theme	highest	1228:1234	arg1	production					1249:1258	NO production	1246:1258	NO production	1246:1258	By contrast, TPPPS1 exhibited other effects, and it induced the highest amount of NO production, thereby indicating that TPPPS1 had the best antioxidant activity.
25782009	1	24	theme	immune	186:191	arg1	responses					193:201	cellular and humoral immune responses	165:201	cellular and humoral immune responses of animals	165:212	Taishan Pinus massoniana pollen polysaccharide (TPPPS) improves cellular and humoral immune responses of animals and is a novel potential immunomodulator.
25782009	9	25	theme	antioxidant	1305:1315	arg1	activity					1317:1324	the best antioxidant activity	1296:1324	the best antioxidant activity	1296:1324	By contrast, TPPPS1 exhibited other effects, and it induced the highest amount of NO production, thereby indicating that TPPPS1 had the best antioxidant activity.
25782009	5	26	theme	polysaccharides	657:671	arg1	kDa					694:696	56, 25, and 128 kDa	678:696	56, 25, and 128 kDa	678:696	The average molecular weights of the three polysaccharides were 56, 25, and 128 kDa, respectively.
25782009	5	26	theme	polysaccharides	657:671	arg1	weights					636:642	The average molecular weights	614:642	The average molecular weights of the three polysaccharides	614:671	The average molecular weights of the three polysaccharides were 56, 25, and 128 kDa, respectively.
25782009	10	27	theme	Leukosis	1408:1415	arg1	ALV-B					1424:1428	ALV-B	1424:1428	ALV-B	1424:1428	TPPPS3 at 50 μg/mL significantly inhibited the proliferation of subgroup B Avian Leukosis virus (ALV-B) through virus adsorption interference in vitro.
25782009	10	27	theme	Leukosis	1408:1415	arg1	virus					1417:1421	subgroup B Avian Leukosis virus	1391:1421	subgroup B Avian Leukosis virus (ALV-B)	1391:1429	TPPPS3 at 50 μg/mL significantly inhibited the proliferation of subgroup B Avian Leukosis virus (ALV-B) through virus adsorption interference in vitro.
25782009	3	28	theme	water	432:436	arg1	extraction					438:447	water extraction	432:447	water extraction	432:447	To investigate the composition of TPPPS, crude polysaccharide was obtained from Taishan P. massoniana pollen through water extraction and ethanol precipitation.
25782009	10	29	theme	virus	1439:1443	arg1	interference					1456:1467	virus adsorption interference	1439:1467	virus adsorption interference	1439:1467	TPPPS3 at 50 μg/mL significantly inhibited the proliferation of subgroup B Avian Leukosis virus (ALV-B) through virus adsorption interference in vitro.
25782009	10	30	theme	Avian	1402:1406	arg1	ALV-B					1424:1428	ALV-B	1424:1428	ALV-B	1424:1428	TPPPS3 at 50 μg/mL significantly inhibited the proliferation of subgroup B Avian Leukosis virus (ALV-B) through virus adsorption interference in vitro.
25782009	10	30	theme	Avian	1402:1406	arg1	virus					1417:1421	subgroup B Avian Leukosis virus	1391:1421	subgroup B Avian Leukosis virus (ALV-B)	1391:1429	TPPPS3 at 50 μg/mL significantly inhibited the proliferation of subgroup B Avian Leukosis virus (ALV-B) through virus adsorption interference in vitro.
25782009	1	31	theme	Taishan	101:107	arg1	TPPPS					149:153	Taishan Pinus massoniana pollen polysaccharide (TPPPS)	101:154	Taishan Pinus massoniana pollen polysaccharide (TPPPS)	101:154	Taishan Pinus massoniana pollen polysaccharide (TPPPS) improves cellular and humoral immune responses of animals and is a novel potential immunomodulator.
25782009	12	32	theme	TPPPS1-3	1736:1743	arg1	proportion					1722:1731	a reasonable proportion	1709:1731	a reasonable proportion of TPPPS1-3	1709:1743	Further studies on the use of a reasonable proportion of TPPPS1-3 may facilitate the development of an effective immunomodulator.
25782009	10	33	theme	B	1400:1400	arg1	ALV-B					1424:1428	ALV-B	1424:1428	ALV-B	1424:1428	TPPPS3 at 50 μg/mL significantly inhibited the proliferation of subgroup B Avian Leukosis virus (ALV-B) through virus adsorption interference in vitro.
25782009	10	33	theme	B	1400:1400	arg1	virus					1417:1421	subgroup B Avian Leukosis virus	1391:1421	subgroup B Avian Leukosis virus (ALV-B)	1391:1429	TPPPS3 at 50 μg/mL significantly inhibited the proliferation of subgroup B Avian Leukosis virus (ALV-B) through virus adsorption interference in vitro.
25782009	1	34	theme	Pinus	109:113	arg1	TPPPS					149:153	Taishan Pinus massoniana pollen polysaccharide (TPPPS)	101:154	Taishan Pinus massoniana pollen polysaccharide (TPPPS)	101:154	Taishan Pinus massoniana pollen polysaccharide (TPPPS) improves cellular and humoral immune responses of animals and is a novel potential immunomodulator.
25782009	10	35	theme	subgroup	1391:1398	arg1	ALV-B					1424:1428	ALV-B	1424:1428	ALV-B	1424:1428	TPPPS3 at 50 μg/mL significantly inhibited the proliferation of subgroup B Avian Leukosis virus (ALV-B) through virus adsorption interference in vitro.
25782009	10	35	theme	subgroup	1391:1398	arg1	virus					1417:1421	subgroup B Avian Leukosis virus	1391:1421	subgroup B Avian Leukosis virus (ALV-B)	1391:1429	TPPPS3 at 50 μg/mL significantly inhibited the proliferation of subgroup B Avian Leukosis virus (ALV-B) through virus adsorption interference in vitro.
25782009	1	36	theme	animals	206:212	arg1	responses					193:201	cellular and humoral immune responses	165:201	cellular and humoral immune responses of animals	165:212	Taishan Pinus massoniana pollen polysaccharide (TPPPS) improves cellular and humoral immune responses of animals and is a novel potential immunomodulator.
25782009	0	37	dep	in	91:92	arg1	vitro					94:98	vitro	94:98	vitro	94:98	Characterization and biological activity of Taishan Pinus massoniana pollen polysaccharide in vitro.
25782009	4	38	theme	DEAE-cellulose	576:589	arg1	chromatography					598:611	DEAE-cellulose column chromatography	576:611	DEAE-cellulose column chromatography	576:611	Three homogeneous polysaccharide fractions (TPPPS1, TPPPS2, and TPPPS3) were purified from TPPPS by DEAE-cellulose column chromatography.
25782009	12	39	theme	effective	1782:1790	arg1	immunomodulator					1792:1806	an effective immunomodulator	1779:1806	an effective immunomodulator	1779:1806	Further studies on the use of a reasonable proportion of TPPPS1-3 may facilitate the development of an effective immunomodulator.
25782009	1	40	theme	massoniana	115:124	arg1	TPPPS					149:153	Taishan Pinus massoniana pollen polysaccharide (TPPPS)	101:154	Taishan Pinus massoniana pollen polysaccharide (TPPPS)	101:154	Taishan Pinus massoniana pollen polysaccharide (TPPPS) improves cellular and humoral immune responses of animals and is a novel potential immunomodulator.
25782009	0	41	theme	Taishan	44:50	arg1	polysaccharide					76:89	Taishan Pinus massoniana pollen polysaccharide	44:89	Taishan Pinus massoniana pollen polysaccharide	44:89	Characterization and biological activity of Taishan Pinus massoniana pollen polysaccharide in vitro.
25782009	8	42	theme	IL-2	1103:1106	arg1	secretion					1081:1089	the secretion	1077:1089	the secretion of cytokine IL-2 and TNF	1077:1114	TPPPS3 enhanced the secretion of cytokine IL-2 and TNF, whereas TPPPS2 mainly elevated IL-2 secretion.
25782009	9	43	contain	had	1292:1294	arg2	activity					1317:1324	the best antioxidant activity	1296:1324	the best antioxidant activity	1296:1324	By contrast, TPPPS1 exhibited other effects, and it induced the highest amount of NO production, thereby indicating that TPPPS1 had the best antioxidant activity.
25782009	9	43	contain	had	1292:1294	arg1	TPPPS1					1285:1290	TPPPS1	1285:1290	TPPPS1	1285:1290	By contrast, TPPPS1 exhibited other effects, and it induced the highest amount of NO production, thereby indicating that TPPPS1 had the best antioxidant activity.
25782009	8	44	theme	cytokine	1094:1101	arg1	IL-2					1103:1106	cytokine IL-2	1094:1106	cytokine IL-2	1094:1106	TPPPS3 enhanced the secretion of cytokine IL-2 and TNF, whereas TPPPS2 mainly elevated IL-2 secretion.
25782009	4	45	theme	homogeneous	482:492	arg1	TPPPS2					528:533	TPPPS2	528:533	TPPPS2	528:533	Three homogeneous polysaccharide fractions (TPPPS1, TPPPS2, and TPPPS3) were purified from TPPPS by DEAE-cellulose column chromatography.
25782009	4	45	theme	homogeneous	482:492	arg1	TPPPS3					540:545	TPPPS3	540:545	TPPPS3	540:545	Three homogeneous polysaccharide fractions (TPPPS1, TPPPS2, and TPPPS3) were purified from TPPPS by DEAE-cellulose column chromatography.
25782009	4	45	theme	homogeneous	482:492	arg1	TPPPS1					520:525	TPPPS1	520:525	TPPPS1	520:525	Three homogeneous polysaccharide fractions (TPPPS1, TPPPS2, and TPPPS3) were purified from TPPPS by DEAE-cellulose column chromatography.
25782009	4	45	theme	homogeneous	482:492	arg1	fractions					509:517	Three homogeneous polysaccharide fractions	476:517	Three homogeneous polysaccharide fractions (TPPPS1, TPPPS2, and TPPPS3)	476:546	Three homogeneous polysaccharide fractions (TPPPS1, TPPPS2, and TPPPS3) were purified from TPPPS by DEAE-cellulose column chromatography.
25782009	0	46	theme	massoniana	58:67	arg1	polysaccharide					76:89	Taishan Pinus massoniana pollen polysaccharide	44:89	Taishan Pinus massoniana pollen polysaccharide	44:89	Characterization and biological activity of Taishan Pinus massoniana pollen polysaccharide in vitro.
25782009	8	47	theme	TNF	1112:1114	arg1	secretion					1081:1089	the secretion	1077:1089	the secretion of cytokine IL-2 and TNF	1077:1114	TPPPS3 enhanced the secretion of cytokine IL-2 and TNF, whereas TPPPS2 mainly elevated IL-2 secretion.
25782009	8	48	dep	enhanced	1068:1075	arg1	whereas					1117:1123	whereas	1117:1123	whereas	1117:1123	TPPPS3 enhanced the secretion of cytokine IL-2 and TNF, whereas TPPPS2 mainly elevated IL-2 secretion.
25782009	1	49	theme	pollen	126:131	arg1	TPPPS					149:153	Taishan Pinus massoniana pollen polysaccharide (TPPPS)	101:154	Taishan Pinus massoniana pollen polysaccharide (TPPPS)	101:154	Taishan Pinus massoniana pollen polysaccharide (TPPPS) improves cellular and humoral immune responses of animals and is a novel potential immunomodulator.
25782009	0	50	theme	Pinus	52:56	arg1	polysaccharide					76:89	Taishan Pinus massoniana pollen polysaccharide	44:89	Taishan Pinus massoniana pollen polysaccharide	44:89	Characterization and biological activity of Taishan Pinus massoniana pollen polysaccharide in vitro.
25782009	12	51	theme	reasonable	1711:1720	arg1	proportion					1722:1731	a reasonable proportion	1709:1731	a reasonable proportion of TPPPS1-3	1709:1743	Further studies on the use of a reasonable proportion of TPPPS1-3 may facilitate the development of an effective immunomodulator.
25782009	6	52	theme	galacturonic	840:851	arg1	acid					853:856	galacturonic acid	840:856	galacturonic acid	840:856	Results of high-performance liquid chromatography (HPLC) showed that TPPPS comprised mannose, ribose, xylose, glucuronic acid, galacturonic acid, glucose, galactose, and arabinose.
25782009	1	53	theme	polysaccharide	133:146	arg1	TPPPS					149:153	Taishan Pinus massoniana pollen polysaccharide (TPPPS)	101:154	Taishan Pinus massoniana pollen polysaccharide (TPPPS)	101:154	Taishan Pinus massoniana pollen polysaccharide (TPPPS) improves cellular and humoral immune responses of animals and is a novel potential immunomodulator.
25782009	7	54	theme	biological	898:907	arg1	assays					918:923	The biological activity assays	894:923	The biological activity assays	894:923	The biological activity assays showed that TPPPS2 and TPPPS3 significantly promoted spleen lymphocyte proliferation, and that TPPPS3 showed better effect than TPPPS2.
25782009	7	55	theme	better	1034:1039	arg1	effect					1041:1046	better effect	1034:1046	better effect	1034:1046	The biological activity assays showed that TPPPS2 and TPPPS3 significantly promoted spleen lymphocyte proliferation, and that TPPPS3 showed better effect than TPPPS2.
25782009	9	56	theme	NO	1246:1247	arg1	production					1249:1258	NO production	1246:1258	NO production	1246:1258	By contrast, TPPPS1 exhibited other effects, and it induced the highest amount of NO production, thereby indicating that TPPPS1 had the best antioxidant activity.
25782009	11	57	theme	antioxidant	1575:1585	arg1	effects					1587:1593	antioxidant effects	1575:1593	antioxidant effects	1575:1593	Results indicated that TPPPS comprised three main components, among which, TPPPS1 mainly showed antioxidant effects, whereas TPPPS2 and TPPPS3 played key roles in immunomodulation, especially TPPPS3.
25782009	7	58	theme	spleen	978:983	arg1	proliferation					996:1008	spleen lymphocyte proliferation	978:1008	spleen lymphocyte proliferation	978:1008	The biological activity assays showed that TPPPS2 and TPPPS3 significantly promoted spleen lymphocyte proliferation, and that TPPPS3 showed better effect than TPPPS2.
25782009	1	59	theme	novel	223:227	arg1	immunomodulator					239:253	a novel potential immunomodulator	221:253	a novel potential immunomodulator	221:253	Taishan Pinus massoniana pollen polysaccharide (TPPPS) improves cellular and humoral immune responses of animals and is a novel potential immunomodulator.
25782009	9	60	theme	production	1249:1258	arg1	amount					1236:1241	the highest amount	1224:1241	the highest amount of NO production	1224:1258	By contrast, TPPPS1 exhibited other effects, and it induced the highest amount of NO production, thereby indicating that TPPPS1 had the best antioxidant activity.
25782009	9	60	theme	production	1249:1258	arg1	production					1249:1258	NO production	1246:1258	NO production	1246:1258	By contrast, TPPPS1 exhibited other effects, and it induced the highest amount of NO production, thereby indicating that TPPPS1 had the best antioxidant activity.
25782009	12	61	from	studies	1687:1693	arg1	use					1702:1704	the use	1698:1704	the use of a reasonable proportion of TPPPS1-3	1698:1743	Further studies on the use of a reasonable proportion of TPPPS1-3 may facilitate the development of an effective immunomodulator.
25782009	12	62	theme	proportion	1722:1731	arg1	use					1702:1704	the use	1698:1704	the use of a reasonable proportion of TPPPS1-3	1698:1743	Further studies on the use of a reasonable proportion of TPPPS1-3 may facilitate the development of an effective immunomodulator.
25782009	1	63	theme	potential	229:237	arg1	immunomodulator					239:253	a novel potential immunomodulator	221:253	a novel potential immunomodulator	221:253	Taishan Pinus massoniana pollen polysaccharide (TPPPS) improves cellular and humoral immune responses of animals and is a novel potential immunomodulator.
25782009	0	64	theme	polysaccharide	76:89	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and biological activity of Taishan Pinus massoniana pollen polysaccharide in vitro.
25782009	0	64	theme	polysaccharide	76:89	arg1	activity					32:39	biological activity	21:39	biological activity of Taishan Pinus massoniana pollen polysaccharide in vitro	21:98	Characterization and biological activity of Taishan Pinus massoniana pollen polysaccharide in vitro.
25782009	6	65	theme	glucuronic	823:832	arg1	acid					834:837	glucuronic acid	823:837	glucuronic acid	823:837	Results of high-performance liquid chromatography (HPLC) showed that TPPPS comprised mannose, ribose, xylose, glucuronic acid, galacturonic acid, glucose, galactose, and arabinose.
25782009	6	66	theme	chromatography	748:761	arg1	Results					713:719	Results	713:719	Results of high-performance liquid chromatography (HPLC)	713:768	Results of high-performance liquid chromatography (HPLC) showed that TPPPS comprised mannose, ribose, xylose, glucuronic acid, galacturonic acid, glucose, galactose, and arabinose.
25782009	3	67	theme	ethanol	453:459	arg1	precipitation					461:473	ethanol precipitation	453:473	ethanol precipitation	453:473	To investigate the composition of TPPPS, crude polysaccharide was obtained from Taishan P. massoniana pollen through water extraction and ethanol precipitation.
25782009	0	68	theme	pollen	69:74	arg1	polysaccharide					76:89	Taishan Pinus massoniana pollen polysaccharide	44:89	Taishan Pinus massoniana pollen polysaccharide	44:89	Characterization and biological activity of Taishan Pinus massoniana pollen polysaccharide in vitro.
25782009	0	69	theme	biological	21:30	arg1	activity					32:39	biological activity	21:39	biological activity of Taishan Pinus massoniana pollen polysaccharide in vitro	21:98	Characterization and biological activity of Taishan Pinus massoniana pollen polysaccharide in vitro.
25782009	6	70	theme	liquid	741:746	arg1	chromatography					748:761	high-performance liquid chromatography	724:761	high-performance liquid chromatography (HPLC)	724:768	Results of high-performance liquid chromatography (HPLC) showed that TPPPS comprised mannose, ribose, xylose, glucuronic acid, galacturonic acid, glucose, galactose, and arabinose.
25782009	6	70	theme	liquid	741:746	arg1	HPLC					764:767	HPLC	764:767	HPLC	764:767	Results of high-performance liquid chromatography (HPLC) showed that TPPPS comprised mannose, ribose, xylose, glucuronic acid, galacturonic acid, glucose, galactose, and arabinose.
25782009	12	71	theme	Further	1679:1685	arg1	studies					1687:1693	Further studies	1679:1693	Further studies on the use of a reasonable proportion of TPPPS1-3	1679:1743	Further studies on the use of a reasonable proportion of TPPPS1-3 may facilitate the development of an effective immunomodulator.
29127410	0	0	theme	cell	109:112	arg1	media					122:126	cell culture media	109:126	cell culture media	109:126	Ultrafiltration combined with size exclusion chromatography efficiently isolates extracellular vesicles from cell culture media for compositional and functional studies.
29127410	5	1	theme	pulse	853:857	arg1	sensing					859:865	tuneable resistive pulse sensing	834:865	tuneable resistive pulse sensing	834:865	EV recovery was estimated by bead-coupled flow cytometry and tuneable resistive pulse sensing.
29127410	8	2	theme	ultracentrifugation	1090:1108	arg1	EVs					1110:1112	ultracentrifugation EVs	1090:1112	ultracentrifugation EVs	1090:1112	UF-SEC EVs and ultracentrifugation EVs showed similar fold-enrichments for biological pathways that were distinct from those of UF-SEC protein.
29127410	3	3	theme	bronchial	567:575	arg1	cells					588:592	BEAS-2B bronchial epithelial cells	559:592	BEAS-2B bronchial epithelial cells	559:592	Conditioned media of BEAS-2B bronchial epithelial cells were concentrated on 10 kDa centrifuge filters, followed by separation of EVs and free protein using sepharose CL-4B SEC.
29127410	6	4	theme	protein	917:923	arg1	fractions					925:933	SEC protein fractions	913:933	SEC protein fractions	913:933	The proteomic composition of EV isolates and SEC protein fractions was characterized by nano LC-MS/MS.
29127410	2	5	theme	exclusion	396:404	arg1	chromatography					406:419	size exclusion chromatography	391:419	size exclusion chromatography (UF-SEC)	391:428	We hypothesized that ultrafiltration followed by size exclusion chromatography (UF-SEC) provides well-matched concentrates of EVs and free secreted molecules for proteomic and functional studies.
29127410	2	5	theme	exclusion	396:404	arg1	UF-SEC					422:427	UF-SEC	422:427	UF-SEC	422:427	We hypothesized that ultrafiltration followed by size exclusion chromatography (UF-SEC) provides well-matched concentrates of EVs and free secreted molecules for proteomic and functional studies.
29127410	9	6	with	Treatment	1219:1227	arg1	type					1287:1290	either type	1280:1290	either type of EV isolate	1280:1304	Treatment of BEAS-2B cells with UF-SEC protein, but not with either type of EV isolate increased the IL-8 concentration in the media whereas EVs, but not protein induced monocyte adhesion to endothelial cells.
29127410	9	6	with	Treatment	1219:1227	arg1	protein					1258:1264	UF-SEC protein	1251:1264	UF-SEC protein	1251:1264	Treatment of BEAS-2B cells with UF-SEC protein, but not with either type of EV isolate increased the IL-8 concentration in the media whereas EVs, but not protein induced monocyte adhesion to endothelial cells.
29127410	5	7	theme	bead-coupled	802:813	arg1	cytometry					820:828	bead-coupled flow cytometry	802:828	bead-coupled flow cytometry	802:828	EV recovery was estimated by bead-coupled flow cytometry and tuneable resistive pulse sensing.
29127410	9	8	theme	isolate	1298:1304	arg1	type					1287:1290	either type	1280:1290	either type of EV isolate	1280:1304	Treatment of BEAS-2B cells with UF-SEC protein, but not with either type of EV isolate increased the IL-8 concentration in the media whereas EVs, but not protein induced monocyte adhesion to endothelial cells.
29127410	8	9	theme	biological	1150:1159	arg1	pathways					1161:1168	biological pathways	1150:1168	biological pathways that were distinct from those of UF-SEC protein	1150:1216	UF-SEC EVs and ultracentrifugation EVs showed similar fold-enrichments for biological pathways that were distinct from those of UF-SEC protein.
29127410	6	10	theme	isolates	900:907	arg1	composition					882:892	The proteomic composition	868:892	The proteomic composition of EV isolates and SEC protein fractions	868:933	The proteomic composition of EV isolates and SEC protein fractions was characterized by nano LC-MS/MS.
29127410	3	11	theme	10 kDa	615:620	arg1	filters					633:639	10 kDa centrifuge filters	615:639	10 kDa centrifuge filters	615:639	Conditioned media of BEAS-2B bronchial epithelial cells were concentrated on 10 kDa centrifuge filters, followed by separation of EVs and free protein using sepharose CL-4B SEC.
29127410	7	12	theme	UF-SEC	971:976	arg1	EVs					978:980	UF-SEC EVs	971:980	UF-SEC EVs	971:980	UF-SEC EVs tended to have a higher yield and EV-to-protein rate of purity than ultracentrifugation EVs.
29127410	1	13	theme	EV-free	296:302	arg1	secretome					304:312	the EV-free secretome	292:312	the EV-free secretome to homeostasis and disease	292:339	Appropriate isolation methods are essential for unravelling the relative contribution of extracellular vesicles (EVs) and the EV-free secretome to homeostasis and disease.
29127410	0	14	theme	culture	114:120	arg1	media					122:126	cell culture media	109:126	cell culture media	109:126	Ultrafiltration combined with size exclusion chromatography efficiently isolates extracellular vesicles from cell culture media for compositional and functional studies.
29127410	9	15	theme	monocyte	1389:1396	arg1	adhesion					1398:1405	monocyte adhesion	1389:1405	monocyte adhesion to endothelial cells	1389:1426	Treatment of BEAS-2B cells with UF-SEC protein, but not with either type of EV isolate increased the IL-8 concentration in the media whereas EVs, but not protein induced monocyte adhesion to endothelial cells.
29127410	6	16	theme	EV	897:898	arg1	isolates					900:907	EV isolates	897:907	EV isolates	897:907	The proteomic composition of EV isolates and SEC protein fractions was characterized by nano LC-MS/MS.
29127410	3	17	theme	centrifuge	622:631	arg1	filters					633:639	10 kDa centrifuge filters	615:639	10 kDa centrifuge filters	615:639	Conditioned media of BEAS-2B bronchial epithelial cells were concentrated on 10 kDa centrifuge filters, followed by separation of EVs and free protein using sepharose CL-4B SEC.
29127410	0	18	theme	compositional	132:144	arg1	studies					161:167	compositional and functional studies	132:167	compositional and functional studies	132:167	Ultrafiltration combined with size exclusion chromatography efficiently isolates extracellular vesicles from cell culture media for compositional and functional studies.
29127410	3	19	dep	concentrated	599:610	arg1	followed					642:649	followed	642:649	followed by separation of EVs and free protein using sepharose CL-4B SEC	642:713	Conditioned media of BEAS-2B bronchial epithelial cells were concentrated on 10 kDa centrifuge filters, followed by separation of EVs and free protein using sepharose CL-4B SEC.
29127410	7	20	contain	have	992:995	arg1	EVs					978:980	UF-SEC EVs	971:980	UF-SEC EVs	971:980	UF-SEC EVs tended to have a higher yield and EV-to-protein rate of purity than ultracentrifugation EVs.
29127410	7	20	contain	have	992:995	arg2	yield					1006:1010	a higher yield	997:1010	a higher yield	997:1010	UF-SEC EVs tended to have a higher yield and EV-to-protein rate of purity than ultracentrifugation EVs.
29127410	7	20	contain	have	992:995	arg2	rate					1030:1033	EV-to-protein rate	1016:1033	EV-to-protein rate	1016:1033	UF-SEC EVs tended to have a higher yield and EV-to-protein rate of purity than ultracentrifugation EVs.
29127410	7	21	theme	purity	1038:1043	arg1	yield					1006:1010	a higher yield	997:1010	a higher yield	997:1010	UF-SEC EVs tended to have a higher yield and EV-to-protein rate of purity than ultracentrifugation EVs.
29127410	7	21	theme	purity	1038:1043	arg1	rate					1030:1033	EV-to-protein rate	1016:1033	EV-to-protein rate	1016:1033	UF-SEC EVs tended to have a higher yield and EV-to-protein rate of purity than ultracentrifugation EVs.
29127410	10	22	theme	functional	1541:1550	arg1	effects					1552:1558	functional effects	1541:1558	functional effects	1541:1558	Thus, UF-SEC is a useful alternative for ultracentrifugation and allows comparing the proteomic composition and functional effects of EVs and free secreted molecules.
29127410	9	23	from	concentration	1325:1337	arg1	media					1346:1350	the media	1342:1350	the media	1342:1350	Treatment of BEAS-2B cells with UF-SEC protein, but not with either type of EV isolate increased the IL-8 concentration in the media whereas EVs, but not protein induced monocyte adhesion to endothelial cells.
29127410	7	24	theme	EV-to-protein	1016:1028	arg1	rate					1030:1033	EV-to-protein rate	1016:1033	EV-to-protein rate	1016:1033	UF-SEC EVs tended to have a higher yield and EV-to-protein rate of purity than ultracentrifugation EVs.
29127410	10	25	theme	proteomic	1515:1523	arg1	composition					1525:1535	proteomic composition	1515:1535	proteomic composition	1515:1535	Thus, UF-SEC is a useful alternative for ultracentrifugation and allows comparing the proteomic composition and functional effects of EVs and free secreted molecules.
29127410	2	26	theme	well-matched	439:450	arg1	concentrates					452:463	well-matched concentrates	439:463	well-matched concentrates of EVs	439:470	We hypothesized that ultrafiltration followed by size exclusion chromatography (UF-SEC) provides well-matched concentrates of EVs and free secreted molecules for proteomic and functional studies.
29127410	2	27	theme	proteomic	504:512	arg1	studies					529:535	proteomic and functional studies	504:535	proteomic and functional studies	504:535	We hypothesized that ultrafiltration followed by size exclusion chromatography (UF-SEC) provides well-matched concentrates of EVs and free secreted molecules for proteomic and functional studies.
29127410	9	28	theme	endothelial	1410:1420	arg1	cells					1422:1426	endothelial cells	1410:1426	endothelial cells	1410:1426	Treatment of BEAS-2B cells with UF-SEC protein, but not with either type of EV isolate increased the IL-8 concentration in the media whereas EVs, but not protein induced monocyte adhesion to endothelial cells.
29127410	10	29	theme	EVs	1563:1565	arg1	composition					1525:1535	proteomic composition	1515:1535	proteomic composition	1515:1535	Thus, UF-SEC is a useful alternative for ultracentrifugation and allows comparing the proteomic composition and functional effects of EVs and free secreted molecules.
29127410	10	29	theme	EVs	1563:1565	arg1	effects					1552:1558	functional effects	1541:1558	functional effects	1541:1558	Thus, UF-SEC is a useful alternative for ultracentrifugation and allows comparing the proteomic composition and functional effects of EVs and free secreted molecules.
29127410	6	30	theme	proteomic	872:880	arg1	composition					882:892	The proteomic composition	868:892	The proteomic composition of EV isolates and SEC protein fractions	868:933	The proteomic composition of EV isolates and SEC protein fractions was characterized by nano LC-MS/MS.
29127410	10	31	theme	molecules	1585:1593	arg1	composition					1525:1535	proteomic composition	1515:1535	proteomic composition	1515:1535	Thus, UF-SEC is a useful alternative for ultracentrifugation and allows comparing the proteomic composition and functional effects of EVs and free secreted molecules.
29127410	10	31	theme	molecules	1585:1593	arg1	effects					1552:1558	functional effects	1541:1558	functional effects	1541:1558	Thus, UF-SEC is a useful alternative for ultracentrifugation and allows comparing the proteomic composition and functional effects of EVs and free secreted molecules.
29127410	10	32	dep	composition	1525:1535	arg1	the					1511:1513	the	1511:1513	the	1511:1513	Thus, UF-SEC is a useful alternative for ultracentrifugation and allows comparing the proteomic composition and functional effects of EVs and free secreted molecules.
29127410	10	33	theme	free	1571:1574	arg1	molecules					1585:1593	free secreted molecules	1571:1593	free secreted molecules	1571:1593	Thus, UF-SEC is a useful alternative for ultracentrifugation and allows comparing the proteomic composition and functional effects of EVs and free secreted molecules.
29127410	8	34	theme	similar	1121:1127	arg1	fold-enrichments					1129:1144	similar fold-enrichments	1121:1144	similar fold-enrichments for biological pathways that were distinct from those of UF-SEC protein	1121:1216	UF-SEC EVs and ultracentrifugation EVs showed similar fold-enrichments for biological pathways that were distinct from those of UF-SEC protein.
29127410	0	35	theme	size	30:33	arg1	chromatography					45:58	size exclusion chromatography	30:58	size exclusion chromatography	30:58	Ultrafiltration combined with size exclusion chromatography efficiently isolates extracellular vesicles from cell culture media for compositional and functional studies.
29127410	0	36	theme	functional	150:159	arg1	studies					161:167	compositional and functional studies	132:167	compositional and functional studies	132:167	Ultrafiltration combined with size exclusion chromatography efficiently isolates extracellular vesicles from cell culture media for compositional and functional studies.
29127410	8	37	theme	UF-SEC	1075:1080	arg1	EVs					1082:1084	UF-SEC EVs	1075:1084	UF-SEC EVs	1075:1084	UF-SEC EVs and ultracentrifugation EVs showed similar fold-enrichments for biological pathways that were distinct from those of UF-SEC protein.
29127410	2	38	theme	free	476:479	arg1	molecules					490:498	free secreted molecules	476:498	free secreted molecules for proteomic and functional studies	476:535	We hypothesized that ultrafiltration followed by size exclusion chromatography (UF-SEC) provides well-matched concentrates of EVs and free secreted molecules for proteomic and functional studies.
29127410	2	39	theme	secreted	481:488	arg1	molecules					490:498	free secreted molecules	476:498	free secreted molecules for proteomic and functional studies	476:535	We hypothesized that ultrafiltration followed by size exclusion chromatography (UF-SEC) provides well-matched concentrates of EVs and free secreted molecules for proteomic and functional studies.
29127410	9	40	theme	IL-8	1320:1323	arg1	concentration					1325:1337	the IL-8 concentration	1316:1337	the IL-8 concentration in the media	1316:1350	Treatment of BEAS-2B cells with UF-SEC protein, but not with either type of EV isolate increased the IL-8 concentration in the media whereas EVs, but not protein induced monocyte adhesion to endothelial cells.
29127410	3	41	theme	epithelial	577:586	arg1	cells					588:592	BEAS-2B bronchial epithelial cells	559:592	BEAS-2B bronchial epithelial cells	559:592	Conditioned media of BEAS-2B bronchial epithelial cells were concentrated on 10 kDa centrifuge filters, followed by separation of EVs and free protein using sepharose CL-4B SEC.
29127410	3	42	theme	cells	588:592	arg1	media					550:554	Conditioned media	538:554	Conditioned media of BEAS-2B bronchial epithelial cells	538:592	Conditioned media of BEAS-2B bronchial epithelial cells were concentrated on 10 kDa centrifuge filters, followed by separation of EVs and free protein using sepharose CL-4B SEC.
29127410	8	43	theme	UF-SEC	1203:1208	arg1	protein					1210:1216	UF-SEC protein	1203:1216	UF-SEC protein	1203:1216	UF-SEC EVs and ultracentrifugation EVs showed similar fold-enrichments for biological pathways that were distinct from those of UF-SEC protein.
29127410	6	44	theme	nano	956:959	arg1	LC-MS/MS					961:968	nano LC-MS/MS	956:968	nano LC-MS/MS	956:968	The proteomic composition of EV isolates and SEC protein fractions was characterized by nano LC-MS/MS.
29127410	3	45	theme	EVs	668:670	arg1	separation					654:663	separation	654:663	separation of EVs and free protein	654:687	Conditioned media of BEAS-2B bronchial epithelial cells were concentrated on 10 kDa centrifuge filters, followed by separation of EVs and free protein using sepharose CL-4B SEC.
29127410	2	46	theme	EVs	468:470	arg1	concentrates					452:463	well-matched concentrates	439:463	well-matched concentrates of EVs	439:470	We hypothesized that ultrafiltration followed by size exclusion chromatography (UF-SEC) provides well-matched concentrates of EVs and free secreted molecules for proteomic and functional studies.
29127410	2	46	theme	EVs	468:470	arg1	molecules					490:498	free secreted molecules	476:498	free secreted molecules for proteomic and functional studies	476:535	We hypothesized that ultrafiltration followed by size exclusion chromatography (UF-SEC) provides well-matched concentrates of EVs and free secreted molecules for proteomic and functional studies.
29127410	0	47	theme	exclusion	35:43	arg1	chromatography					45:58	size exclusion chromatography	30:58	size exclusion chromatography	30:58	Ultrafiltration combined with size exclusion chromatography efficiently isolates extracellular vesicles from cell culture media for compositional and functional studies.
29127410	10	48	theme	secreted	1576:1583	arg1	molecules					1585:1593	free secreted molecules	1571:1593	free secreted molecules	1571:1593	Thus, UF-SEC is a useful alternative for ultracentrifugation and allows comparing the proteomic composition and functional effects of EVs and free secreted molecules.
29127410	3	49	theme	CL-4B	705:709	arg1	SEC					711:713	sepharose CL-4B SEC	695:713	sepharose CL-4B SEC	695:713	Conditioned media of BEAS-2B bronchial epithelial cells were concentrated on 10 kDa centrifuge filters, followed by separation of EVs and free protein using sepharose CL-4B SEC.
29127410	9	50	theme	BEAS-2B	1232:1238	arg1	cells					1240:1244	BEAS-2B cells	1232:1244	BEAS-2B cells	1232:1244	Treatment of BEAS-2B cells with UF-SEC protein, but not with either type of EV isolate increased the IL-8 concentration in the media whereas EVs, but not protein induced monocyte adhesion to endothelial cells.
29127410	1	51	theme	extracellular	259:271	arg1	EVs					283:285	EVs	283:285	EVs	283:285	Appropriate isolation methods are essential for unravelling the relative contribution of extracellular vesicles (EVs) and the EV-free secretome to homeostasis and disease.
29127410	1	51	theme	extracellular	259:271	arg1	vesicles					273:280	extracellular vesicles	259:280	extracellular vesicles (EVs)	259:286	Appropriate isolation methods are essential for unravelling the relative contribution of extracellular vesicles (EVs) and the EV-free secretome to homeostasis and disease.
29127410	2	52	theme	functional	518:527	arg1	studies					529:535	proteomic and functional studies	504:535	proteomic and functional studies	504:535	We hypothesized that ultrafiltration followed by size exclusion chromatography (UF-SEC) provides well-matched concentrates of EVs and free secreted molecules for proteomic and functional studies.
29127410	5	53	theme	resistive	843:851	arg1	sensing					859:865	tuneable resistive pulse sensing	834:865	tuneable resistive pulse sensing	834:865	EV recovery was estimated by bead-coupled flow cytometry and tuneable resistive pulse sensing.
29127410	9	54	theme	cells	1240:1244	arg1	Treatment					1219:1227	Treatment	1219:1227	Treatment of BEAS-2B cells with UF-SEC protein, but not with either type of EV isolate	1219:1304	Treatment of BEAS-2B cells with UF-SEC protein, but not with either type of EV isolate increased the IL-8 concentration in the media whereas EVs, but not protein induced monocyte adhesion to endothelial cells.
29127410	2	55	theme	size	391:394	arg1	chromatography					406:419	size exclusion chromatography	391:419	size exclusion chromatography (UF-SEC)	391:428	We hypothesized that ultrafiltration followed by size exclusion chromatography (UF-SEC) provides well-matched concentrates of EVs and free secreted molecules for proteomic and functional studies.
29127410	2	55	theme	size	391:394	arg1	UF-SEC					422:427	UF-SEC	422:427	UF-SEC	422:427	We hypothesized that ultrafiltration followed by size exclusion chromatography (UF-SEC) provides well-matched concentrates of EVs and free secreted molecules for proteomic and functional studies.
29127410	9	56	theme	EV	1295:1296	arg1	isolate					1298:1304	EV isolate	1295:1304	EV isolate	1295:1304	Treatment of BEAS-2B cells with UF-SEC protein, but not with either type of EV isolate increased the IL-8 concentration in the media whereas EVs, but not protein induced monocyte adhesion to endothelial cells.
29127410	9	57	theme	UF-SEC	1251:1256	arg1	protein					1258:1264	UF-SEC protein	1251:1264	UF-SEC protein	1251:1264	Treatment of BEAS-2B cells with UF-SEC protein, but not with either type of EV isolate increased the IL-8 concentration in the media whereas EVs, but not protein induced monocyte adhesion to endothelial cells.
29127410	3	58	theme	BEAS-2B	559:565	arg1	cells					588:592	BEAS-2B bronchial epithelial cells	559:592	BEAS-2B bronchial epithelial cells	559:592	Conditioned media of BEAS-2B bronchial epithelial cells were concentrated on 10 kDa centrifuge filters, followed by separation of EVs and free protein using sepharose CL-4B SEC.
29127410	6	59	theme	fractions	925:933	arg1	composition					882:892	The proteomic composition	868:892	The proteomic composition of EV isolates and SEC protein fractions	868:933	The proteomic composition of EV isolates and SEC protein fractions was characterized by nano LC-MS/MS.
29127410	7	60	theme	ultracentrifugation	1050:1068	arg1	EVs					1070:1072	ultracentrifugation EVs	1050:1072	ultracentrifugation EVs	1050:1072	UF-SEC EVs tended to have a higher yield and EV-to-protein rate of purity than ultracentrifugation EVs.
29127410	3	61	theme	Conditioned	538:548	arg1	media					550:554	Conditioned media	538:554	Conditioned media of BEAS-2B bronchial epithelial cells	538:592	Conditioned media of BEAS-2B bronchial epithelial cells were concentrated on 10 kDa centrifuge filters, followed by separation of EVs and free protein using sepharose CL-4B SEC.
29127410	7	62	theme	higher	999:1004	arg1	yield					1006:1010	a higher yield	997:1010	a higher yield	997:1010	UF-SEC EVs tended to have a higher yield and EV-to-protein rate of purity than ultracentrifugation EVs.
29127410	1	63	theme	vesicles	273:280	arg1	contribution					243:254	the relative contribution	230:254	the relative contribution of extracellular vesicles (EVs)	230:286	Appropriate isolation methods are essential for unravelling the relative contribution of extracellular vesicles (EVs) and the EV-free secretome to homeostasis and disease.
29127410	1	63	theme	vesicles	273:280	arg1	secretome					304:312	the EV-free secretome	292:312	the EV-free secretome to homeostasis and disease	292:339	Appropriate isolation methods are essential for unravelling the relative contribution of extracellular vesicles (EVs) and the EV-free secretome to homeostasis and disease.
29127410	5	64	theme	EV	773:774	arg1	recovery					776:783	EV recovery	773:783	EV recovery	773:783	EV recovery was estimated by bead-coupled flow cytometry and tuneable resistive pulse sensing.
29127410	0	65	from	media	122:126	arg1	isolates					72:79	isolates	72:79	isolates	72:79	Ultrafiltration combined with size exclusion chromatography efficiently isolates extracellular vesicles from cell culture media for compositional and functional studies.
29127410	3	66	theme	sepharose	695:703	arg1	SEC					711:713	sepharose CL-4B SEC	695:713	sepharose CL-4B SEC	695:713	Conditioned media of BEAS-2B bronchial epithelial cells were concentrated on 10 kDa centrifuge filters, followed by separation of EVs and free protein using sepharose CL-4B SEC.
29127410	10	67	theme	useful	1447:1452	arg1	alternative					1454:1464	a useful alternative	1445:1464	a useful alternative for ultracentrifugation	1445:1488	Thus, UF-SEC is a useful alternative for ultracentrifugation and allows comparing the proteomic composition and functional effects of EVs and free secreted molecules.
29127410	10	67	theme	useful	1447:1452	arg1	UF-SEC					1435:1440	UF-SEC	1435:1440	UF-SEC	1435:1440	Thus, UF-SEC is a useful alternative for ultracentrifugation and allows comparing the proteomic composition and functional effects of EVs and free secreted molecules.
29127410	5	68	theme	tuneable	834:841	arg1	sensing					859:865	tuneable resistive pulse sensing	834:865	tuneable resistive pulse sensing	834:865	EV recovery was estimated by bead-coupled flow cytometry and tuneable resistive pulse sensing.
29127410	6	69	theme	SEC	913:915	arg1	fractions					925:933	SEC protein fractions	913:933	SEC protein fractions	913:933	The proteomic composition of EV isolates and SEC protein fractions was characterized by nano LC-MS/MS.
29127410	3	70	theme	free	676:679	arg1	protein					681:687	free protein	676:687	free protein	676:687	Conditioned media of BEAS-2B bronchial epithelial cells were concentrated on 10 kDa centrifuge filters, followed by separation of EVs and free protein using sepharose CL-4B SEC.
29127410	1	71	theme	relative	234:241	arg1	contribution					243:254	the relative contribution	230:254	the relative contribution of extracellular vesicles (EVs)	230:286	Appropriate isolation methods are essential for unravelling the relative contribution of extracellular vesicles (EVs) and the EV-free secretome to homeostasis and disease.
29127410	1	72	theme	Appropriate	170:180	arg1	methods					192:198	Appropriate isolation methods	170:198	Appropriate isolation methods	170:198	Appropriate isolation methods are essential for unravelling the relative contribution of extracellular vesicles (EVs) and the EV-free secretome to homeostasis and disease.
29127410	8	73	from	those	1194:1198	arg1	distinct					1180:1187	distinct	1180:1187	distinct	1180:1187	UF-SEC EVs and ultracentrifugation EVs showed similar fold-enrichments for biological pathways that were distinct from those of UF-SEC protein.
29127410	0	74	theme	extracellular	81:93	arg1	vesicles					95:102	extracellular vesicles	81:102	extracellular vesicles	81:102	Ultrafiltration combined with size exclusion chromatography efficiently isolates extracellular vesicles from cell culture media for compositional and functional studies.
29127410	3	75	theme	protein	681:687	arg1	separation					654:663	separation	654:663	separation of EVs and free protein	654:687	Conditioned media of BEAS-2B bronchial epithelial cells were concentrated on 10 kDa centrifuge filters, followed by separation of EVs and free protein using sepharose CL-4B SEC.
29127410	1	76	theme	isolation	182:190	arg1	methods					192:198	Appropriate isolation methods	170:198	Appropriate isolation methods	170:198	Appropriate isolation methods are essential for unravelling the relative contribution of extracellular vesicles (EVs) and the EV-free secretome to homeostasis and disease.
29127410	5	77	theme	flow	815:818	arg1	cytometry					820:828	bead-coupled flow cytometry	802:828	bead-coupled flow cytometry	802:828	EV recovery was estimated by bead-coupled flow cytometry and tuneable resistive pulse sensing.
24812231	9	0	theme	litter	1530:1535	arg1	moisture					1537:1544	litter moisture	1530:1544	litter moisture	1530:1544	Fine grinding of the oat hulls diminished the effect on litter moisture and water activity.
24812231	5	1	with	treatment	929:937	arg1	birds					946:950	6 birds	944:950	6 birds per replicate	944:964	The 8 treatments were randomly assigned to cages within blocks, resulting in 12 replicates per treatment with 6 birds per replicate.
24812231	2	2	theme	moisture	351:358	arg1	content					360:366	moisture content	351:366	moisture content	351:366	The current experiment was designed to measure the effects of 8 different dietary characteristics on litter and excreta moisture content.
24812231	7	3	theme	moisture	1329:1336	arg1	content					1338:1344	litter moisture content	1322:1344	litter moisture content	1322:1344	Increasing dietary NSP content by feeding a corn-based diet (low NSP) compared with a wheat-based diet (high NSP) increased water intake, excreta moisture and free water, and litter moisture content.
24812231	4	4	theme	particle	586:593	arg1	size					595:598	particle size	586:598	particle size of insoluble fiber	586:617	The dietary treatments consisted of nonstarch polysaccharide content (NSP; corn vs. wheat), particle size of insoluble fiber (coarse vs. finely ground oat hulls), viscosity of a nonfermentable fiber (low- and high-viscosity carboxymethyl cellulose), inclusion of a clay mineral (sepiolite), and inclusion of a laxative electrolyte (MgSO4).
24812231	2	5	dep	litter	332:337	arg1	content					360:366	moisture content	351:366	moisture content	351:366	The current experiment was designed to measure the effects of 8 different dietary characteristics on litter and excreta moisture content.
24812231	4	6	theme	fiber	687:691	arg1	inclusion					789:797	inclusion	789:797	inclusion of a laxative electrolyte (MgSO4)	789:831	The dietary treatments consisted of nonstarch polysaccharide content (NSP; corn vs. wheat), particle size of insoluble fiber (coarse vs. finely ground oat hulls), viscosity of a nonfermentable fiber (low- and high-viscosity carboxymethyl cellulose), inclusion of a clay mineral (sepiolite), and inclusion of a laxative electrolyte (MgSO4).
24812231	4	6	theme	fiber	687:691	arg1	inclusion					744:752	inclusion	744:752	inclusion of a clay mineral (sepiolite)	744:782	The dietary treatments consisted of nonstarch polysaccharide content (NSP; corn vs. wheat), particle size of insoluble fiber (coarse vs. finely ground oat hulls), viscosity of a nonfermentable fiber (low- and high-viscosity carboxymethyl cellulose), inclusion of a clay mineral (sepiolite), and inclusion of a laxative electrolyte (MgSO4).
24812231	4	6	theme	fiber	687:691	arg1	viscosity					657:665	viscosity	657:665	viscosity of a nonfermentable fiber	657:691	The dietary treatments consisted of nonstarch polysaccharide content (NSP; corn vs. wheat), particle size of insoluble fiber (coarse vs. finely ground oat hulls), viscosity of a nonfermentable fiber (low- and high-viscosity carboxymethyl cellulose), inclusion of a clay mineral (sepiolite), and inclusion of a laxative electrolyte (MgSO4).
24812231	4	6	theme	fiber	687:691	arg1	size					595:598	particle size	586:598	particle size of insoluble fiber	586:617	The dietary treatments consisted of nonstarch polysaccharide content (NSP; corn vs. wheat), particle size of insoluble fiber (coarse vs. finely ground oat hulls), viscosity of a nonfermentable fiber (low- and high-viscosity carboxymethyl cellulose), inclusion of a clay mineral (sepiolite), and inclusion of a laxative electrolyte (MgSO4).
24812231	4	6	theme	fiber	687:691	arg1	content					555:561	nonstarch polysaccharide content	530:561	nonstarch polysaccharide content (NSP; corn vs. wheat)	530:583	The dietary treatments consisted of nonstarch polysaccharide content (NSP; corn vs. wheat), particle size of insoluble fiber (coarse vs. finely ground oat hulls), viscosity of a nonfermentable fiber (low- and high-viscosity carboxymethyl cellulose), inclusion of a clay mineral (sepiolite), and inclusion of a laxative electrolyte (MgSO4).
24812231	4	6	theme	fiber	687:691	arg1	cellulose					732:740	low- and high-viscosity carboxymethyl cellulose	694:740	low- and high-viscosity carboxymethyl cellulose	694:740	The dietary treatments consisted of nonstarch polysaccharide content (NSP; corn vs. wheat), particle size of insoluble fiber (coarse vs. finely ground oat hulls), viscosity of a nonfermentable fiber (low- and high-viscosity carboxymethyl cellulose), inclusion of a clay mineral (sepiolite), and inclusion of a laxative electrolyte (MgSO4).
24812231	6	7	theme	treatments	998:1007	arg1	effects					975:981	Limited effects	967:981	Limited effects of the dietary treatments	967:1007	Limited effects of the dietary treatments were noted on excreta and litter water activity, and indications were observed that this measurement is limited in high-moisture samples.
24812231	4	8	theme	oat	645:647	arg1	hulls					649:653	ground oat hulls	638:653	ground oat hulls	638:653	The dietary treatments consisted of nonstarch polysaccharide content (NSP; corn vs. wheat), particle size of insoluble fiber (coarse vs. finely ground oat hulls), viscosity of a nonfermentable fiber (low- and high-viscosity carboxymethyl cellulose), inclusion of a clay mineral (sepiolite), and inclusion of a laxative electrolyte (MgSO4).
24812231	4	9	theme	laxative	804:811	arg1	MgSO4					826:830	MgSO4	826:830	MgSO4	826:830	The dietary treatments consisted of nonstarch polysaccharide content (NSP; corn vs. wheat), particle size of insoluble fiber (coarse vs. finely ground oat hulls), viscosity of a nonfermentable fiber (low- and high-viscosity carboxymethyl cellulose), inclusion of a clay mineral (sepiolite), and inclusion of a laxative electrolyte (MgSO4).
24812231	4	9	theme	laxative	804:811	arg1	electrolyte					813:823	a laxative electrolyte	802:823	a laxative electrolyte (MgSO4)	802:831	The dietary treatments consisted of nonstarch polysaccharide content (NSP; corn vs. wheat), particle size of insoluble fiber (coarse vs. finely ground oat hulls), viscosity of a nonfermentable fiber (low- and high-viscosity carboxymethyl cellulose), inclusion of a clay mineral (sepiolite), and inclusion of a laxative electrolyte (MgSO4).
24812231	2	10	theme	dietary	305:311	arg1	characteristics					313:327	8 different dietary characteristics	293:327	8 different dietary characteristics	293:327	The current experiment was designed to measure the effects of 8 different dietary characteristics on litter and excreta moisture content.
24812231	12	11	theme	free	1866:1869	arg1	measurement					1877:1887	excreta free water measurement	1858:1887	excreta free water measurement	1858:1887	Findings of the current experiment indicate a potential for excreta free water measurement as an additional parameter to assess excreta quality besides total moisture.
24812231	0	12	theme	broiler	113:119	arg1	samples					140:146	broiler excreta and litter samples	113:146	broiler excreta and litter samples	113:146	Evaluation of free water and water activity measurements as functional alternatives to total moisture content in broiler excreta and litter samples.
24812231	10	13	theme	water	1601:1605	arg1	content					1607:1613	excreta moisture and free water content	1575:1613	content	1607:1613	However, excreta moisture and free water content were similar when fed finely or coarsely ground oat hulls.
24812231	12	14	theme	experiment	1822:1831	arg1	Findings					1798:1805	Findings	1798:1805	Findings of the current experiment	1798:1831	Findings of the current experiment indicate a potential for excreta free water measurement as an additional parameter to assess excreta quality besides total moisture.
24812231	7	15	theme	excreta	1285:1291	arg1	moisture					1293:1300	excreta moisture	1285:1300	excreta moisture	1285:1300	Increasing dietary NSP content by feeding a corn-based diet (low NSP) compared with a wheat-based diet (high NSP) increased water intake, excreta moisture and free water, and litter moisture content.
24812231	1	16	theme	poultry	216:222	arg1	barns					224:228	practical poultry barns	206:228	practical poultry barns	206:228	Litter moisture contents vary greatly between and within practical poultry barns.
24812231	8	17	theme	insoluble	1354:1362	arg1	fibers					1364:1369	insoluble fibers	1354:1369	insoluble fibers	1354:1369	Adding insoluble fibers to the wheat-based diet reduced excreta and litter moisture content, as well as litter water activity.
24812231	5	18	dep	12	911:912	arg1	replicates					914:923	replicates	914:923	replicates	914:923	The 8 treatments were randomly assigned to cages within blocks, resulting in 12 replicates per treatment with 6 birds per replicate.
24812231	0	19	from	content	102:108	arg1	samples					140:146	broiler excreta and litter samples	113:146	broiler excreta and litter samples	113:146	Evaluation of free water and water activity measurements as functional alternatives to total moisture content in broiler excreta and litter samples.
24812231	4	20	theme	low-	694:697	arg1	content					555:561	nonstarch polysaccharide content	530:561	nonstarch polysaccharide content (NSP; corn vs. wheat)	530:583	The dietary treatments consisted of nonstarch polysaccharide content (NSP; corn vs. wheat), particle size of insoluble fiber (coarse vs. finely ground oat hulls), viscosity of a nonfermentable fiber (low- and high-viscosity carboxymethyl cellulose), inclusion of a clay mineral (sepiolite), and inclusion of a laxative electrolyte (MgSO4).
24812231	4	20	theme	low-	694:697	arg1	cellulose					732:740	low- and high-viscosity carboxymethyl cellulose	694:740	low- and high-viscosity carboxymethyl cellulose	694:740	The dietary treatments consisted of nonstarch polysaccharide content (NSP; corn vs. wheat), particle size of insoluble fiber (coarse vs. finely ground oat hulls), viscosity of a nonfermentable fiber (low- and high-viscosity carboxymethyl cellulose), inclusion of a clay mineral (sepiolite), and inclusion of a laxative electrolyte (MgSO4).
24812231	8	21	theme	wheat-based	1378:1388	arg1	diet					1390:1393	the wheat-based diet	1374:1393	the wheat-based diet	1374:1393	Adding insoluble fibers to the wheat-based diet reduced excreta and litter moisture content, as well as litter water activity.
24812231	6	22	theme	high-moisture	1124:1136	arg1	samples					1138:1144	high-moisture samples	1124:1144	high-moisture samples	1124:1144	Limited effects of the dietary treatments were noted on excreta and litter water activity, and indications were observed that this measurement is limited in high-moisture samples.
24812231	4	23	theme	dietary	498:504	arg1	treatments					506:515	The dietary treatments	494:515	The dietary treatments	494:515	The dietary treatments consisted of nonstarch polysaccharide content (NSP; corn vs. wheat), particle size of insoluble fiber (coarse vs. finely ground oat hulls), viscosity of a nonfermentable fiber (low- and high-viscosity carboxymethyl cellulose), inclusion of a clay mineral (sepiolite), and inclusion of a laxative electrolyte (MgSO4).
24812231	12	24	theme	total	1950:1954	arg1	moisture					1956:1963	total moisture	1950:1963	total moisture	1950:1963	Findings of the current experiment indicate a potential for excreta free water measurement as an additional parameter to assess excreta quality besides total moisture.
24812231	7	25	theme	dietary	1158:1164	arg1	content					1170:1176	dietary NSP content	1158:1176	dietary NSP content	1158:1176	Increasing dietary NSP content by feeding a corn-based diet (low NSP) compared with a wheat-based diet (high NSP) increased water intake, excreta moisture and free water, and litter moisture content.
24812231	1	26	theme	moisture	156:163	arg1	contents					165:172	Litter moisture contents	149:172	Litter moisture contents	149:172	Litter moisture contents vary greatly between and within practical poultry barns.
24812231	0	27	theme	total	87:91	arg1	content					102:108	total moisture content	87:108	total moisture content in broiler excreta and litter samples	87:146	Evaluation of free water and water activity measurements as functional alternatives to total moisture content in broiler excreta and litter samples.
24812231	9	28	from	effect	1520:1525	arg1	moisture					1537:1544	litter moisture	1530:1544	litter moisture	1530:1544	Fine grinding of the oat hulls diminished the effect on litter moisture and water activity.
24812231	9	28	from	effect	1520:1525	arg1	activity					1556:1563	water activity	1550:1563	water activity	1550:1563	Fine grinding of the oat hulls diminished the effect on litter moisture and water activity.
24812231	8	29	theme	moisture	1422:1429	arg1	content					1431:1437	excreta and litter moisture content	1403:1437	excreta and litter moisture content	1403:1437	Adding insoluble fibers to the wheat-based diet reduced excreta and litter moisture content, as well as litter water activity.
24812231	11	30	theme	previous	1780:1787	arg1	studies					1789:1795	previous studies	1780:1795	previous studies	1780:1795	The effects of changing viscosity and adding a clay mineral or laxative deviated from results observed in previous studies.
24812231	7	31	theme	high	1251:1254	arg1	diet					1245:1248	a wheat-based diet	1231:1248	a wheat-based diet (high NSP) increased water intake, excreta moisture and free water, and litter moisture content	1231:1344	Increasing dietary NSP content by feeding a corn-based diet (low NSP) compared with a wheat-based diet (high NSP) increased water intake, excreta moisture and free water, and litter moisture content.
24812231	7	31	theme	high	1251:1254	arg1	NSP					1256:1258	high NSP	1251:1258	high NSP	1251:1258	Increasing dietary NSP content by feeding a corn-based diet (low NSP) compared with a wheat-based diet (high NSP) increased water intake, excreta moisture and free water, and litter moisture content.
24812231	3	32	theme	water	388:392	arg1	content					394:400	free water content	383:400	free water content	383:400	Additionally, free water content and water activity of the excreta and litter were evaluated as additional quality measures.
24812231	3	32	theme	water	388:392	arg1	activity					412:419	water activity	406:419	water activity	406:419	Additionally, free water content and water activity of the excreta and litter were evaluated as additional quality measures.
24812231	3	32	theme	water	388:392	arg1	measures					484:491	additional quality measures	465:491	additional quality measures	465:491	Additionally, free water content and water activity of the excreta and litter were evaluated as additional quality measures.
24812231	0	33	theme	functional	60:69	arg1	alternatives					71:82	functional alternatives	60:82	functional alternatives to total moisture content in broiler excreta and litter samples	60:146	Evaluation of free water and water activity measurements as functional alternatives to total moisture content in broiler excreta and litter samples.
24812231	4	34	theme	high-viscosity	703:716	arg1	content					555:561	nonstarch polysaccharide content	530:561	nonstarch polysaccharide content (NSP; corn vs. wheat)	530:583	The dietary treatments consisted of nonstarch polysaccharide content (NSP; corn vs. wheat), particle size of insoluble fiber (coarse vs. finely ground oat hulls), viscosity of a nonfermentable fiber (low- and high-viscosity carboxymethyl cellulose), inclusion of a clay mineral (sepiolite), and inclusion of a laxative electrolyte (MgSO4).
24812231	4	34	theme	high-viscosity	703:716	arg1	cellulose					732:740	low- and high-viscosity carboxymethyl cellulose	694:740	low- and high-viscosity carboxymethyl cellulose	694:740	The dietary treatments consisted of nonstarch polysaccharide content (NSP; corn vs. wheat), particle size of insoluble fiber (coarse vs. finely ground oat hulls), viscosity of a nonfermentable fiber (low- and high-viscosity carboxymethyl cellulose), inclusion of a clay mineral (sepiolite), and inclusion of a laxative electrolyte (MgSO4).
24812231	6	35	theme	dietary	990:996	arg1	treatments					998:1007	the dietary treatments	986:1007	the dietary treatments	986:1007	Limited effects of the dietary treatments were noted on excreta and litter water activity, and indications were observed that this measurement is limited in high-moisture samples.
24812231	0	36	theme	free	14:17	arg1	water					19:23	free water	14:23	free water	14:23	Evaluation of free water and water activity measurements as functional alternatives to total moisture content in broiler excreta and litter samples.
24812231	8	37	theme	litter	1451:1456	arg1	activity					1464:1471	litter water activity	1451:1471	litter water activity	1451:1471	Adding insoluble fibers to the wheat-based diet reduced excreta and litter moisture content, as well as litter water activity.
24812231	3	38	dep	excreta	428:434	arg1	the					424:426	the	424:426	the	424:426	Additionally, free water content and water activity of the excreta and litter were evaluated as additional quality measures.
24812231	6	39	theme	Limited	967:973	arg1	effects					975:981	Limited effects	967:981	Limited effects of the dietary treatments	967:1007	Limited effects of the dietary treatments were noted on excreta and litter water activity, and indications were observed that this measurement is limited in high-moisture samples.
24812231	4	40	theme	nonstarch	530:538	arg1	content					555:561	nonstarch polysaccharide content	530:561	nonstarch polysaccharide content (NSP; corn vs. wheat)	530:583	The dietary treatments consisted of nonstarch polysaccharide content (NSP; corn vs. wheat), particle size of insoluble fiber (coarse vs. finely ground oat hulls), viscosity of a nonfermentable fiber (low- and high-viscosity carboxymethyl cellulose), inclusion of a clay mineral (sepiolite), and inclusion of a laxative electrolyte (MgSO4).
24812231	4	40	theme	nonstarch	530:538	arg1	cellulose					732:740	low- and high-viscosity carboxymethyl cellulose	694:740	low- and high-viscosity carboxymethyl cellulose	694:740	The dietary treatments consisted of nonstarch polysaccharide content (NSP; corn vs. wheat), particle size of insoluble fiber (coarse vs. finely ground oat hulls), viscosity of a nonfermentable fiber (low- and high-viscosity carboxymethyl cellulose), inclusion of a clay mineral (sepiolite), and inclusion of a laxative electrolyte (MgSO4).
24812231	10	41	theme	free	1596:1599	arg1	content					1607:1613	excreta moisture and free water content	1575:1613	content	1607:1613	However, excreta moisture and free water content were similar when fed finely or coarsely ground oat hulls.
24812231	0	42	theme	activity	35:42	arg1	measurements					44:55	water activity measurements	29:55	water activity measurements	29:55	Evaluation of free water and water activity measurements as functional alternatives to total moisture content in broiler excreta and litter samples.
24812231	9	43	theme	hulls	1499:1503	arg1	grinding					1479:1486	grinding	1479:1486	grinding	1479:1486	Fine grinding of the oat hulls diminished the effect on litter moisture and water activity.
24812231	4	44	theme	clay	759:762	arg1	inclusion					789:797	inclusion	789:797	inclusion of a laxative electrolyte (MgSO4)	789:831	The dietary treatments consisted of nonstarch polysaccharide content (NSP; corn vs. wheat), particle size of insoluble fiber (coarse vs. finely ground oat hulls), viscosity of a nonfermentable fiber (low- and high-viscosity carboxymethyl cellulose), inclusion of a clay mineral (sepiolite), and inclusion of a laxative electrolyte (MgSO4).
24812231	4	44	theme	clay	759:762	arg1	inclusion					744:752	inclusion	744:752	inclusion of a clay mineral (sepiolite)	744:782	The dietary treatments consisted of nonstarch polysaccharide content (NSP; corn vs. wheat), particle size of insoluble fiber (coarse vs. finely ground oat hulls), viscosity of a nonfermentable fiber (low- and high-viscosity carboxymethyl cellulose), inclusion of a clay mineral (sepiolite), and inclusion of a laxative electrolyte (MgSO4).
24812231	4	44	theme	clay	759:762	arg1	viscosity					657:665	viscosity	657:665	viscosity of a nonfermentable fiber	657:691	The dietary treatments consisted of nonstarch polysaccharide content (NSP; corn vs. wheat), particle size of insoluble fiber (coarse vs. finely ground oat hulls), viscosity of a nonfermentable fiber (low- and high-viscosity carboxymethyl cellulose), inclusion of a clay mineral (sepiolite), and inclusion of a laxative electrolyte (MgSO4).
24812231	4	44	theme	clay	759:762	arg1	size					595:598	particle size	586:598	particle size of insoluble fiber	586:617	The dietary treatments consisted of nonstarch polysaccharide content (NSP; corn vs. wheat), particle size of insoluble fiber (coarse vs. finely ground oat hulls), viscosity of a nonfermentable fiber (low- and high-viscosity carboxymethyl cellulose), inclusion of a clay mineral (sepiolite), and inclusion of a laxative electrolyte (MgSO4).
24812231	4	44	theme	clay	759:762	arg1	content					555:561	nonstarch polysaccharide content	530:561	nonstarch polysaccharide content (NSP; corn vs. wheat)	530:583	The dietary treatments consisted of nonstarch polysaccharide content (NSP; corn vs. wheat), particle size of insoluble fiber (coarse vs. finely ground oat hulls), viscosity of a nonfermentable fiber (low- and high-viscosity carboxymethyl cellulose), inclusion of a clay mineral (sepiolite), and inclusion of a laxative electrolyte (MgSO4).
24812231	4	44	theme	clay	759:762	arg1	cellulose					732:740	low- and high-viscosity carboxymethyl cellulose	694:740	low- and high-viscosity carboxymethyl cellulose	694:740	The dietary treatments consisted of nonstarch polysaccharide content (NSP; corn vs. wheat), particle size of insoluble fiber (coarse vs. finely ground oat hulls), viscosity of a nonfermentable fiber (low- and high-viscosity carboxymethyl cellulose), inclusion of a clay mineral (sepiolite), and inclusion of a laxative electrolyte (MgSO4).
24812231	3	45	theme	quality	476:482	arg1	content					394:400	free water content	383:400	free water content	383:400	Additionally, free water content and water activity of the excreta and litter were evaluated as additional quality measures.
24812231	3	45	theme	quality	476:482	arg1	activity					412:419	water activity	406:419	water activity	406:419	Additionally, free water content and water activity of the excreta and litter were evaluated as additional quality measures.
24812231	3	45	theme	quality	476:482	arg1	measures					484:491	additional quality measures	465:491	additional quality measures	465:491	Additionally, free water content and water activity of the excreta and litter were evaluated as additional quality measures.
24812231	6	46	theme	water	1042:1046	arg1	activity					1048:1055	litter water activity	1035:1055	litter water activity	1035:1055	Limited effects of the dietary treatments were noted on excreta and litter water activity, and indications were observed that this measurement is limited in high-moisture samples.
24812231	12	47	theme	additional	1895:1904	arg1	parameter					1906:1914	an additional parameter	1892:1914	an additional parameter to assess excreta quality besides total moisture	1892:1963	Findings of the current experiment indicate a potential for excreta free water measurement as an additional parameter to assess excreta quality besides total moisture.
24812231	7	48	theme	corn-based	1191:1200	arg1	diet					1202:1205	a corn-based diet	1189:1205	a corn-based diet (low NSP) compared with a wheat-based diet (high NSP) increased water intake, excreta moisture and free water, and litter moisture content	1189:1344	Increasing dietary NSP content by feeding a corn-based diet (low NSP) compared with a wheat-based diet (high NSP) increased water intake, excreta moisture and free water, and litter moisture content.
24812231	7	48	theme	corn-based	1191:1200	arg1	NSP					1212:1214	low NSP	1208:1214	low NSP	1208:1214	Increasing dietary NSP content by feeding a corn-based diet (low NSP) compared with a wheat-based diet (high NSP) increased water intake, excreta moisture and free water, and litter moisture content.
24812231	4	49	theme	insoluble	603:611	arg1	fiber					613:617	insoluble fiber	603:617	insoluble fiber	603:617	The dietary treatments consisted of nonstarch polysaccharide content (NSP; corn vs. wheat), particle size of insoluble fiber (coarse vs. finely ground oat hulls), viscosity of a nonfermentable fiber (low- and high-viscosity carboxymethyl cellulose), inclusion of a clay mineral (sepiolite), and inclusion of a laxative electrolyte (MgSO4).
24812231	4	50	theme	electrolyte	813:823	arg1	inclusion					789:797	inclusion	789:797	inclusion of a laxative electrolyte (MgSO4)	789:831	The dietary treatments consisted of nonstarch polysaccharide content (NSP; corn vs. wheat), particle size of insoluble fiber (coarse vs. finely ground oat hulls), viscosity of a nonfermentable fiber (low- and high-viscosity carboxymethyl cellulose), inclusion of a clay mineral (sepiolite), and inclusion of a laxative electrolyte (MgSO4).
24812231	4	50	theme	electrolyte	813:823	arg1	inclusion					744:752	inclusion	744:752	inclusion of a clay mineral (sepiolite)	744:782	The dietary treatments consisted of nonstarch polysaccharide content (NSP; corn vs. wheat), particle size of insoluble fiber (coarse vs. finely ground oat hulls), viscosity of a nonfermentable fiber (low- and high-viscosity carboxymethyl cellulose), inclusion of a clay mineral (sepiolite), and inclusion of a laxative electrolyte (MgSO4).
24812231	4	50	theme	electrolyte	813:823	arg1	viscosity					657:665	viscosity	657:665	viscosity of a nonfermentable fiber	657:691	The dietary treatments consisted of nonstarch polysaccharide content (NSP; corn vs. wheat), particle size of insoluble fiber (coarse vs. finely ground oat hulls), viscosity of a nonfermentable fiber (low- and high-viscosity carboxymethyl cellulose), inclusion of a clay mineral (sepiolite), and inclusion of a laxative electrolyte (MgSO4).
24812231	4	50	theme	electrolyte	813:823	arg1	size					595:598	particle size	586:598	particle size of insoluble fiber	586:617	The dietary treatments consisted of nonstarch polysaccharide content (NSP; corn vs. wheat), particle size of insoluble fiber (coarse vs. finely ground oat hulls), viscosity of a nonfermentable fiber (low- and high-viscosity carboxymethyl cellulose), inclusion of a clay mineral (sepiolite), and inclusion of a laxative electrolyte (MgSO4).
24812231	4	50	theme	electrolyte	813:823	arg1	content					555:561	nonstarch polysaccharide content	530:561	nonstarch polysaccharide content (NSP; corn vs. wheat)	530:583	The dietary treatments consisted of nonstarch polysaccharide content (NSP; corn vs. wheat), particle size of insoluble fiber (coarse vs. finely ground oat hulls), viscosity of a nonfermentable fiber (low- and high-viscosity carboxymethyl cellulose), inclusion of a clay mineral (sepiolite), and inclusion of a laxative electrolyte (MgSO4).
24812231	4	50	theme	electrolyte	813:823	arg1	cellulose					732:740	low- and high-viscosity carboxymethyl cellulose	694:740	low- and high-viscosity carboxymethyl cellulose	694:740	The dietary treatments consisted of nonstarch polysaccharide content (NSP; corn vs. wheat), particle size of insoluble fiber (coarse vs. finely ground oat hulls), viscosity of a nonfermentable fiber (low- and high-viscosity carboxymethyl cellulose), inclusion of a clay mineral (sepiolite), and inclusion of a laxative electrolyte (MgSO4).
24812231	12	51	theme	water	1871:1875	arg1	measurement					1877:1887	excreta free water measurement	1858:1887	excreta free water measurement	1858:1887	Findings of the current experiment indicate a potential for excreta free water measurement as an additional parameter to assess excreta quality besides total moisture.
24812231	9	52	theme	water	1550:1554	arg1	activity					1556:1563	water activity	1550:1563	water activity	1550:1563	Fine grinding of the oat hulls diminished the effect on litter moisture and water activity.
24812231	11	53	theme	mineral	1726:1732	arg1	laxative					1737:1744	a clay mineral or laxative	1719:1744	a clay mineral or laxative	1719:1744	The effects of changing viscosity and adding a clay mineral or laxative deviated from results observed in previous studies.
24812231	7	54	theme	litter	1322:1327	arg1	content					1338:1344	litter moisture content	1322:1344	litter moisture content	1322:1344	Increasing dietary NSP content by feeding a corn-based diet (low NSP) compared with a wheat-based diet (high NSP) increased water intake, excreta moisture and free water, and litter moisture content.
24812231	4	55	theme	ground	638:643	arg1	hulls					649:653	ground oat hulls	638:653	ground oat hulls	638:653	The dietary treatments consisted of nonstarch polysaccharide content (NSP; corn vs. wheat), particle size of insoluble fiber (coarse vs. finely ground oat hulls), viscosity of a nonfermentable fiber (low- and high-viscosity carboxymethyl cellulose), inclusion of a clay mineral (sepiolite), and inclusion of a laxative electrolyte (MgSO4).
24812231	2	56	theme	characteristics	313:327	arg1	effects					282:288	the effects	278:288	the effects of 8 different dietary characteristics on litter and excreta moisture content	278:366	The current experiment was designed to measure the effects of 8 different dietary characteristics on litter and excreta moisture content.
24812231	0	57	theme	excreta	121:127	arg1	samples					140:146	broiler excreta and litter samples	113:146	broiler excreta and litter samples	113:146	Evaluation of free water and water activity measurements as functional alternatives to total moisture content in broiler excreta and litter samples.
24812231	2	58	theme	different	295:303	arg1	characteristics					313:327	8 different dietary characteristics	293:327	8 different dietary characteristics	293:327	The current experiment was designed to measure the effects of 8 different dietary characteristics on litter and excreta moisture content.
24812231	10	59	theme	oat	1663:1665	arg1	hulls					1667:1671	ground oat hulls	1656:1671	ground oat hulls	1656:1671	However, excreta moisture and free water content were similar when fed finely or coarsely ground oat hulls.
24812231	7	60	theme	free	1306:1309	arg1	water					1311:1315	free water	1306:1315	free water	1306:1315	Increasing dietary NSP content by feeding a corn-based diet (low NSP) compared with a wheat-based diet (high NSP) increased water intake, excreta moisture and free water, and litter moisture content.
24812231	0	61	theme	litter	133:138	arg1	samples					140:146	broiler excreta and litter samples	113:146	broiler excreta and litter samples	113:146	Evaluation of free water and water activity measurements as functional alternatives to total moisture content in broiler excreta and litter samples.
24812231	13	62	theme	further	2015:2021	arg1	investigation					2023:2035	further investigation	2015:2035	further investigation	2015:2035	The exact implication of this parameter warrants further investigation.
24812231	2	63	theme	current	235:241	arg1	experiment					243:252	The current experiment	231:252	The current experiment	231:252	The current experiment was designed to measure the effects of 8 different dietary characteristics on litter and excreta moisture content.
24812231	1	64	theme	practical	206:214	arg1	barns					224:228	practical poultry barns	206:228	practical poultry barns	206:228	Litter moisture contents vary greatly between and within practical poultry barns.
24812231	12	65	theme	current	1814:1820	arg1	experiment					1822:1831	the current experiment	1810:1831	the current experiment	1810:1831	Findings of the current experiment indicate a potential for excreta free water measurement as an additional parameter to assess excreta quality besides total moisture.
24812231	4	66	theme	nonfermentable	672:685	arg1	fiber					687:691	a nonfermentable fiber	670:691	a nonfermentable fiber	670:691	The dietary treatments consisted of nonstarch polysaccharide content (NSP; corn vs. wheat), particle size of insoluble fiber (coarse vs. finely ground oat hulls), viscosity of a nonfermentable fiber (low- and high-viscosity carboxymethyl cellulose), inclusion of a clay mineral (sepiolite), and inclusion of a laxative electrolyte (MgSO4).
24812231	10	67	theme	ground	1656:1661	arg1	hulls					1667:1671	ground oat hulls	1656:1671	ground oat hulls	1656:1671	However, excreta moisture and free water content were similar when fed finely or coarsely ground oat hulls.
24812231	7	68	theme	water	1271:1275	arg1	intake					1277:1282	water intake	1271:1282	water intake	1271:1282	Increasing dietary NSP content by feeding a corn-based diet (low NSP) compared with a wheat-based diet (high NSP) increased water intake, excreta moisture and free water, and litter moisture content.
24812231	7	69	theme	NSP	1166:1168	arg1	content					1170:1176	dietary NSP content	1158:1176	dietary NSP content	1158:1176	Increasing dietary NSP content by feeding a corn-based diet (low NSP) compared with a wheat-based diet (high NSP) increased water intake, excreta moisture and free water, and litter moisture content.
24812231	13	70	theme	parameter	1996:2004	arg1	implication					1976:1986	The exact implication	1966:1986	The exact implication of this parameter	1966:2004	The exact implication of this parameter warrants further investigation.
24812231	2	71	from	effects	282:288	arg1	excreta					343:349	excreta	343:349	excreta	343:349	The current experiment was designed to measure the effects of 8 different dietary characteristics on litter and excreta moisture content.
24812231	2	71	from	effects	282:288	arg1	litter					332:337	litter	332:337	litter	332:337	The current experiment was designed to measure the effects of 8 different dietary characteristics on litter and excreta moisture content.
24812231	8	72	theme	excreta	1403:1409	arg1	content					1431:1437	excreta and litter moisture content	1403:1437	excreta and litter moisture content	1403:1437	Adding insoluble fibers to the wheat-based diet reduced excreta and litter moisture content, as well as litter water activity.
24812231	12	73	theme	excreta	1858:1864	arg1	measurement					1877:1887	excreta free water measurement	1858:1887	excreta free water measurement	1858:1887	Findings of the current experiment indicate a potential for excreta free water measurement as an additional parameter to assess excreta quality besides total moisture.
24812231	12	74	theme	excreta	1926:1932	arg1	quality					1934:1940	excreta quality	1926:1940	excreta quality	1926:1940	Findings of the current experiment indicate a potential for excreta free water measurement as an additional parameter to assess excreta quality besides total moisture.
24812231	4	75	dep	content	555:561	arg1	hulls					649:653	ground oat hulls	638:653	ground oat hulls	638:653	The dietary treatments consisted of nonstarch polysaccharide content (NSP; corn vs. wheat), particle size of insoluble fiber (coarse vs. finely ground oat hulls), viscosity of a nonfermentable fiber (low- and high-viscosity carboxymethyl cellulose), inclusion of a clay mineral (sepiolite), and inclusion of a laxative electrolyte (MgSO4).
24812231	4	75	dep	content	555:561	arg1	coarse					620:625	coarse	620:625	coarse	620:625	The dietary treatments consisted of nonstarch polysaccharide content (NSP; corn vs. wheat), particle size of insoluble fiber (coarse vs. finely ground oat hulls), viscosity of a nonfermentable fiber (low- and high-viscosity carboxymethyl cellulose), inclusion of a clay mineral (sepiolite), and inclusion of a laxative electrolyte (MgSO4).
24812231	4	75	dep	content	555:561	arg1	NSP					564:566	NSP	564:566	NSP; corn vs. wheat	564:582	The dietary treatments consisted of nonstarch polysaccharide content (NSP; corn vs. wheat), particle size of insoluble fiber (coarse vs. finely ground oat hulls), viscosity of a nonfermentable fiber (low- and high-viscosity carboxymethyl cellulose), inclusion of a clay mineral (sepiolite), and inclusion of a laxative electrolyte (MgSO4).
24812231	1	76	theme	Litter	149:154	arg1	contents					165:172	Litter moisture contents	149:172	Litter moisture contents	149:172	Litter moisture contents vary greatly between and within practical poultry barns.
24812231	8	77	theme	litter	1415:1420	arg1	content					1431:1437	excreta and litter moisture content	1403:1437	excreta and litter moisture content	1403:1437	Adding insoluble fibers to the wheat-based diet reduced excreta and litter moisture content, as well as litter water activity.
24812231	0	78	theme	moisture	93:100	arg1	content					102:108	total moisture content	87:108	total moisture content in broiler excreta and litter samples	87:146	Evaluation of free water and water activity measurements as functional alternatives to total moisture content in broiler excreta and litter samples.
24812231	13	79	theme	exact	1970:1974	arg1	implication					1976:1986	The exact implication	1966:1986	The exact implication of this parameter	1966:2004	The exact implication of this parameter warrants further investigation.
24812231	11	80	theme	clay	1721:1724	arg1	laxative					1737:1744	a clay mineral or laxative	1719:1744	a clay mineral or laxative	1719:1744	The effects of changing viscosity and adding a clay mineral or laxative deviated from results observed in previous studies.
24812231	3	81	theme	litter	440:445	arg1	content					394:400	free water content	383:400	free water content	383:400	Additionally, free water content and water activity of the excreta and litter were evaluated as additional quality measures.
24812231	3	81	theme	litter	440:445	arg1	activity					412:419	water activity	406:419	water activity	406:419	Additionally, free water content and water activity of the excreta and litter were evaluated as additional quality measures.
24812231	3	81	theme	litter	440:445	arg1	measures					484:491	additional quality measures	465:491	additional quality measures	465:491	Additionally, free water content and water activity of the excreta and litter were evaluated as additional quality measures.
24812231	4	82	dep	NSP	564:566	arg1	wheat					578:582	wheat	578:582	wheat	578:582	The dietary treatments consisted of nonstarch polysaccharide content (NSP; corn vs. wheat), particle size of insoluble fiber (coarse vs. finely ground oat hulls), viscosity of a nonfermentable fiber (low- and high-viscosity carboxymethyl cellulose), inclusion of a clay mineral (sepiolite), and inclusion of a laxative electrolyte (MgSO4).
24812231	4	82	dep	NSP	564:566	arg1	corn					569:572	corn	569:572	corn	569:572	The dietary treatments consisted of nonstarch polysaccharide content (NSP; corn vs. wheat), particle size of insoluble fiber (coarse vs. finely ground oat hulls), viscosity of a nonfermentable fiber (low- and high-viscosity carboxymethyl cellulose), inclusion of a clay mineral (sepiolite), and inclusion of a laxative electrolyte (MgSO4).
24812231	7	83	theme	wheat-based	1233:1243	arg1	diet					1245:1248	a wheat-based diet	1231:1248	a wheat-based diet (high NSP) increased water intake, excreta moisture and free water, and litter moisture content	1231:1344	Increasing dietary NSP content by feeding a corn-based diet (low NSP) compared with a wheat-based diet (high NSP) increased water intake, excreta moisture and free water, and litter moisture content.
24812231	7	83	theme	wheat-based	1233:1243	arg1	NSP					1256:1258	high NSP	1251:1258	high NSP	1251:1258	Increasing dietary NSP content by feeding a corn-based diet (low NSP) compared with a wheat-based diet (high NSP) increased water intake, excreta moisture and free water, and litter moisture content.
24812231	3	84	theme	excreta	428:434	arg1	content					394:400	free water content	383:400	free water content	383:400	Additionally, free water content and water activity of the excreta and litter were evaluated as additional quality measures.
24812231	3	84	theme	excreta	428:434	arg1	activity					412:419	water activity	406:419	water activity	406:419	Additionally, free water content and water activity of the excreta and litter were evaluated as additional quality measures.
24812231	3	84	theme	excreta	428:434	arg1	measures					484:491	additional quality measures	465:491	additional quality measures	465:491	Additionally, free water content and water activity of the excreta and litter were evaluated as additional quality measures.
24812231	0	85	theme	water	19:23	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of free water and water activity measurements as functional alternatives to total moisture content in broiler excreta and litter samples.	0:147	Evaluation of free water and water activity measurements as functional alternatives to total moisture content in broiler excreta and litter samples.
24812231	3	86	theme	free	383:386	arg1	content					394:400	free water content	383:400	free water content	383:400	Additionally, free water content and water activity of the excreta and litter were evaluated as additional quality measures.
24812231	3	86	theme	free	383:386	arg1	activity					412:419	water activity	406:419	water activity	406:419	Additionally, free water content and water activity of the excreta and litter were evaluated as additional quality measures.
24812231	3	86	theme	free	383:386	arg1	measures					484:491	additional quality measures	465:491	additional quality measures	465:491	Additionally, free water content and water activity of the excreta and litter were evaluated as additional quality measures.
24812231	8	87	theme	water	1458:1462	arg1	activity					1464:1471	litter water activity	1451:1471	litter water activity	1451:1471	Adding insoluble fibers to the wheat-based diet reduced excreta and litter moisture content, as well as litter water activity.
24812231	4	88	theme	carboxymethyl	718:730	arg1	content					555:561	nonstarch polysaccharide content	530:561	nonstarch polysaccharide content (NSP; corn vs. wheat)	530:583	The dietary treatments consisted of nonstarch polysaccharide content (NSP; corn vs. wheat), particle size of insoluble fiber (coarse vs. finely ground oat hulls), viscosity of a nonfermentable fiber (low- and high-viscosity carboxymethyl cellulose), inclusion of a clay mineral (sepiolite), and inclusion of a laxative electrolyte (MgSO4).
24812231	4	88	theme	carboxymethyl	718:730	arg1	cellulose					732:740	low- and high-viscosity carboxymethyl cellulose	694:740	low- and high-viscosity carboxymethyl cellulose	694:740	The dietary treatments consisted of nonstarch polysaccharide content (NSP; corn vs. wheat), particle size of insoluble fiber (coarse vs. finely ground oat hulls), viscosity of a nonfermentable fiber (low- and high-viscosity carboxymethyl cellulose), inclusion of a clay mineral (sepiolite), and inclusion of a laxative electrolyte (MgSO4).
24812231	3	89	theme	additional	465:474	arg1	content					394:400	free water content	383:400	free water content	383:400	Additionally, free water content and water activity of the excreta and litter were evaluated as additional quality measures.
24812231	3	89	theme	additional	465:474	arg1	activity					412:419	water activity	406:419	water activity	406:419	Additionally, free water content and water activity of the excreta and litter were evaluated as additional quality measures.
24812231	3	89	theme	additional	465:474	arg1	measures					484:491	additional quality measures	465:491	additional quality measures	465:491	Additionally, free water content and water activity of the excreta and litter were evaluated as additional quality measures.
24812231	0	90	theme	water	29:33	arg1	measurements					44:55	water activity measurements	29:55	water activity measurements	29:55	Evaluation of free water and water activity measurements as functional alternatives to total moisture content in broiler excreta and litter samples.
24812231	4	91	theme	polysaccharide	540:553	arg1	content					555:561	nonstarch polysaccharide content	530:561	nonstarch polysaccharide content (NSP; corn vs. wheat)	530:583	The dietary treatments consisted of nonstarch polysaccharide content (NSP; corn vs. wheat), particle size of insoluble fiber (coarse vs. finely ground oat hulls), viscosity of a nonfermentable fiber (low- and high-viscosity carboxymethyl cellulose), inclusion of a clay mineral (sepiolite), and inclusion of a laxative electrolyte (MgSO4).
24812231	4	91	theme	polysaccharide	540:553	arg1	cellulose					732:740	low- and high-viscosity carboxymethyl cellulose	694:740	low- and high-viscosity carboxymethyl cellulose	694:740	The dietary treatments consisted of nonstarch polysaccharide content (NSP; corn vs. wheat), particle size of insoluble fiber (coarse vs. finely ground oat hulls), viscosity of a nonfermentable fiber (low- and high-viscosity carboxymethyl cellulose), inclusion of a clay mineral (sepiolite), and inclusion of a laxative electrolyte (MgSO4).
24812231	0	92	theme	measurements	44:55	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of free water and water activity measurements as functional alternatives to total moisture content in broiler excreta and litter samples.	0:147	Evaluation of free water and water activity measurements as functional alternatives to total moisture content in broiler excreta and litter samples.
24812231	9	93	theme	oat	1495:1497	arg1	hulls					1499:1503	the oat hulls	1491:1503	the oat hulls	1491:1503	Fine grinding of the oat hulls diminished the effect on litter moisture and water activity.
24812231	7	94	theme	low	1208:1210	arg1	diet					1202:1205	a corn-based diet	1189:1205	a corn-based diet (low NSP) compared with a wheat-based diet (high NSP) increased water intake, excreta moisture and free water, and litter moisture content	1189:1344	Increasing dietary NSP content by feeding a corn-based diet (low NSP) compared with a wheat-based diet (high NSP) increased water intake, excreta moisture and free water, and litter moisture content.
24812231	7	94	theme	low	1208:1210	arg1	NSP					1212:1214	low NSP	1208:1214	low NSP	1208:1214	Increasing dietary NSP content by feeding a corn-based diet (low NSP) compared with a wheat-based diet (high NSP) increased water intake, excreta moisture and free water, and litter moisture content.
24812231	3	95	theme	water	406:410	arg1	content					394:400	free water content	383:400	free water content	383:400	Additionally, free water content and water activity of the excreta and litter were evaluated as additional quality measures.
24812231	3	95	theme	water	406:410	arg1	activity					412:419	water activity	406:419	water activity	406:419	Additionally, free water content and water activity of the excreta and litter were evaluated as additional quality measures.
24812231	3	95	theme	water	406:410	arg1	measures					484:491	additional quality measures	465:491	additional quality measures	465:491	Additionally, free water content and water activity of the excreta and litter were evaluated as additional quality measures.
24812231	11	96	theme	or	1734:1735	arg1	laxative					1737:1744	a clay mineral or laxative	1719:1744	a clay mineral or laxative	1719:1744	The effects of changing viscosity and adding a clay mineral or laxative deviated from results observed in previous studies.
24812231	11	97	located	observed	1768:1775	arg2	results					1760:1766	results	1760:1766	results observed in previous studies	1760:1795	The effects of changing viscosity and adding a clay mineral or laxative deviated from results observed in previous studies.
24812231	11	97	located	observed	1768:1775	arg1	studies					1789:1795	previous studies	1780:1795	previous studies	1780:1795	The effects of changing viscosity and adding a clay mineral or laxative deviated from results observed in previous studies.
24812231	10	98	theme	excreta	1575:1581	arg1	moisture					1583:1590	excreta moisture and free water content	1575:1613	moisture	1583:1590	However, excreta moisture and free water content were similar when fed finely or coarsely ground oat hulls.
24812231	6	99	theme	litter	1035:1040	arg1	activity					1048:1055	litter water activity	1035:1055	litter water activity	1035:1055	Limited effects of the dietary treatments were noted on excreta and litter water activity, and indications were observed that this measurement is limited in high-moisture samples.
24812231	4	100	theme	fiber	613:617	arg1	inclusion					789:797	inclusion	789:797	inclusion of a laxative electrolyte (MgSO4)	789:831	The dietary treatments consisted of nonstarch polysaccharide content (NSP; corn vs. wheat), particle size of insoluble fiber (coarse vs. finely ground oat hulls), viscosity of a nonfermentable fiber (low- and high-viscosity carboxymethyl cellulose), inclusion of a clay mineral (sepiolite), and inclusion of a laxative electrolyte (MgSO4).
24812231	4	100	theme	fiber	613:617	arg1	inclusion					744:752	inclusion	744:752	inclusion of a clay mineral (sepiolite)	744:782	The dietary treatments consisted of nonstarch polysaccharide content (NSP; corn vs. wheat), particle size of insoluble fiber (coarse vs. finely ground oat hulls), viscosity of a nonfermentable fiber (low- and high-viscosity carboxymethyl cellulose), inclusion of a clay mineral (sepiolite), and inclusion of a laxative electrolyte (MgSO4).
24812231	4	100	theme	fiber	613:617	arg1	viscosity					657:665	viscosity	657:665	viscosity of a nonfermentable fiber	657:691	The dietary treatments consisted of nonstarch polysaccharide content (NSP; corn vs. wheat), particle size of insoluble fiber (coarse vs. finely ground oat hulls), viscosity of a nonfermentable fiber (low- and high-viscosity carboxymethyl cellulose), inclusion of a clay mineral (sepiolite), and inclusion of a laxative electrolyte (MgSO4).
24812231	4	100	theme	fiber	613:617	arg1	size					595:598	particle size	586:598	particle size of insoluble fiber	586:617	The dietary treatments consisted of nonstarch polysaccharide content (NSP; corn vs. wheat), particle size of insoluble fiber (coarse vs. finely ground oat hulls), viscosity of a nonfermentable fiber (low- and high-viscosity carboxymethyl cellulose), inclusion of a clay mineral (sepiolite), and inclusion of a laxative electrolyte (MgSO4).
24812231	4	100	theme	fiber	613:617	arg1	content					555:561	nonstarch polysaccharide content	530:561	nonstarch polysaccharide content (NSP; corn vs. wheat)	530:583	The dietary treatments consisted of nonstarch polysaccharide content (NSP; corn vs. wheat), particle size of insoluble fiber (coarse vs. finely ground oat hulls), viscosity of a nonfermentable fiber (low- and high-viscosity carboxymethyl cellulose), inclusion of a clay mineral (sepiolite), and inclusion of a laxative electrolyte (MgSO4).
24812231	4	100	theme	fiber	613:617	arg1	cellulose					732:740	low- and high-viscosity carboxymethyl cellulose	694:740	low- and high-viscosity carboxymethyl cellulose	694:740	The dietary treatments consisted of nonstarch polysaccharide content (NSP; corn vs. wheat), particle size of insoluble fiber (coarse vs. finely ground oat hulls), viscosity of a nonfermentable fiber (low- and high-viscosity carboxymethyl cellulose), inclusion of a clay mineral (sepiolite), and inclusion of a laxative electrolyte (MgSO4).
24812231	4	101	theme	mineral	764:770	arg1	clay					759:762	a clay mineral	757:770	a clay mineral (sepiolite)	757:782	The dietary treatments consisted of nonstarch polysaccharide content (NSP; corn vs. wheat), particle size of insoluble fiber (coarse vs. finely ground oat hulls), viscosity of a nonfermentable fiber (low- and high-viscosity carboxymethyl cellulose), inclusion of a clay mineral (sepiolite), and inclusion of a laxative electrolyte (MgSO4).
24812231	4	101	theme	mineral	764:770	arg1	sepiolite					773:781	sepiolite	773:781	sepiolite	773:781	The dietary treatments consisted of nonstarch polysaccharide content (NSP; corn vs. wheat), particle size of insoluble fiber (coarse vs. finely ground oat hulls), viscosity of a nonfermentable fiber (low- and high-viscosity carboxymethyl cellulose), inclusion of a clay mineral (sepiolite), and inclusion of a laxative electrolyte (MgSO4).
28940006	0	0	theme	Drosera	97:103	arg1	capensis					105:112	Drosera capensis	97:112	Drosera capensis	97:112	Occurrence of myo-inositol and alkyl-substituted polysaccharide in the prey-trapping mucilage of Drosera capensis.
28940006	7	1	theme	mucilage	1237:1244	arg1	components					1219:1228	the mineral components	1207:1228	the mineral components of the mucilage	1207:1244	A re-evaluation of the mineral components of the mucilage, reported 40 years ago, is presented from the viewpoints of the current result and plants' natural habitat.
28940006	2	2	theme	myo-inositol	389:400	arg1	myo-inositol					389:400	myo-inositol	389:400	myo-inositol	389:400	The mucilage was found to contain beside a very large molecular weight polysaccharide a significant amount of myo-inositol.
28940006	2	2	theme	myo-inositol	389:400	arg1	amount					379:384	a significant amount	365:384	beside a very large molecular weight polysaccharide a significant amount of myo-inositol	313:400	The mucilage was found to contain beside a very large molecular weight polysaccharide a significant amount of myo-inositol.
28940006	6	3	theme	hydrophobic	1044:1054	arg1	parts					1068:1072	the hydrophobic insect body parts	1040:1072	the hydrophobic insect body parts	1040:1072	These lipophilic moieties may provide the prey-trapping mucilage with the unique adhesive property onto the hydrophobic insect body parts, as well as onto the nature's well-known superhydrophobic surfaces such as the leaves of the sacred lotus plants.
28940006	6	4	theme	lipophilic	942:951	arg1	moieties					953:960	These lipophilic moieties	936:960	These lipophilic moieties	936:960	These lipophilic moieties may provide the prey-trapping mucilage with the unique adhesive property onto the hydrophobic insect body parts, as well as onto the nature's well-known superhydrophobic surfaces such as the leaves of the sacred lotus plants.
28940006	3	5	with	dialysis	542:549	arg1	alcohol					599:605	alcohol	599:605	alcohol	599:605	It appears that myo-inositol escaped detection due to the commonly applied methodology on the chemical analysis of plant mucilage, such as dialysis, precipitation of polysaccharide component with alcohol, acid hydrolysis and detection of the resultant monosaccharide (aldose) units.
28940006	2	6	dep	beside	313:318	arg1	polysaccharide					350:363	a very large molecular weight polysaccharide	320:363	beside a very large molecular weight polysaccharide a significant amount of myo-inositol	313:400	The mucilage was found to contain beside a very large molecular weight polysaccharide a significant amount of myo-inositol.
28940006	0	7	from	Occurrence	0:9	arg1	mucilage					85:92	the prey-trapping mucilage	67:92	the prey-trapping mucilage of Drosera capensis	67:112	Occurrence of myo-inositol and alkyl-substituted polysaccharide in the prey-trapping mucilage of Drosera capensis.
28940006	4	8	from	tentacles	800:808	arg1	functions					699:707	The possible functions	686:707	The possible functions of myo-inositol in the mucilage droplets	686:748	The possible functions of myo-inositol in the mucilage droplets and the fate after being washed off from the leaf tentacles are proposed.
28940006	4	8	from	tentacles	800:808	arg1	fate					758:761	the fate	754:761	the fate after being washed off from the leaf tentacles	754:808	The possible functions of myo-inositol in the mucilage droplets and the fate after being washed off from the leaf tentacles are proposed.
28940006	3	9	theme	component	584:592	arg1	dialysis					542:549	dialysis	542:549	dialysis	542:549	It appears that myo-inositol escaped detection due to the commonly applied methodology on the chemical analysis of plant mucilage, such as dialysis, precipitation of polysaccharide component with alcohol, acid hydrolysis and detection of the resultant monosaccharide (aldose) units.
28940006	3	9	theme	component	584:592	arg1	hydrolysis					613:622	acid hydrolysis	608:622	acid hydrolysis	608:622	It appears that myo-inositol escaped detection due to the commonly applied methodology on the chemical analysis of plant mucilage, such as dialysis, precipitation of polysaccharide component with alcohol, acid hydrolysis and detection of the resultant monosaccharide (aldose) units.
28940006	3	9	theme	component	584:592	arg1	detection					628:636	detection	628:636	detection of the resultant monosaccharide (aldose) units	628:683	It appears that myo-inositol escaped detection due to the commonly applied methodology on the chemical analysis of plant mucilage, such as dialysis, precipitation of polysaccharide component with alcohol, acid hydrolysis and detection of the resultant monosaccharide (aldose) units.
28940006	3	9	theme	component	584:592	arg1	precipitation					552:564	precipitation	552:564	precipitation of polysaccharide component with alcohol	552:605	It appears that myo-inositol escaped detection due to the commonly applied methodology on the chemical analysis of plant mucilage, such as dialysis, precipitation of polysaccharide component with alcohol, acid hydrolysis and detection of the resultant monosaccharide (aldose) units.
28940006	6	10	theme	sacred	1167:1172	arg1	plants					1180:1185	the sacred lotus plants	1163:1185	the sacred lotus plants	1163:1185	These lipophilic moieties may provide the prey-trapping mucilage with the unique adhesive property onto the hydrophobic insect body parts, as well as onto the nature's well-known superhydrophobic surfaces such as the leaves of the sacred lotus plants.
28940006	0	11	theme	capensis	105:112	arg1	mucilage					85:92	the prey-trapping mucilage	67:92	the prey-trapping mucilage of Drosera capensis	67:112	Occurrence of myo-inositol and alkyl-substituted polysaccharide in the prey-trapping mucilage of Drosera capensis.
28940006	3	12	with	precipitation	552:564	arg1	alcohol					599:605	alcohol	599:605	alcohol	599:605	It appears that myo-inositol escaped detection due to the commonly applied methodology on the chemical analysis of plant mucilage, such as dialysis, precipitation of polysaccharide component with alcohol, acid hydrolysis and detection of the resultant monosaccharide (aldose) units.
28940006	8	13	theme	well-studied	1387:1398	arg1	materials					1419:1427	the well-studied plant mucilaginous materials	1383:1427	the well-studied plant mucilaginous materials	1383:1427	A case for re-examination of the well-studied plant mucilaginous materials is made in light of the new findings.
28940006	3	14	theme	mucilage	524:531	arg1	analysis					506:513	the chemical analysis	493:513	the chemical analysis of plant mucilage	493:531	It appears that myo-inositol escaped detection due to the commonly applied methodology on the chemical analysis of plant mucilage, such as dialysis, precipitation of polysaccharide component with alcohol, acid hydrolysis and detection of the resultant monosaccharide (aldose) units.
28940006	4	15	theme	mucilage	732:739	arg1	droplets					741:748	the mucilage droplets	728:748	the mucilage droplets	728:748	The possible functions of myo-inositol in the mucilage droplets and the fate after being washed off from the leaf tentacles are proposed.
28940006	6	16	theme	well-known	1104:1113	arg1	surfaces					1132:1139	the nature's well-known superhydrophobic surfaces	1091:1139	the nature's well-known superhydrophobic surfaces such as the leaves of the sacred lotus plants	1091:1185	These lipophilic moieties may provide the prey-trapping mucilage with the unique adhesive property onto the hydrophobic insect body parts, as well as onto the nature's well-known superhydrophobic surfaces such as the leaves of the sacred lotus plants.
28940006	6	16	theme	well-known	1104:1113	arg1	the					1149:1151	the	1149:1151	the	1149:1151	These lipophilic moieties may provide the prey-trapping mucilage with the unique adhesive property onto the hydrophobic insect body parts, as well as onto the nature's well-known superhydrophobic surfaces such as the leaves of the sacred lotus plants.
28940006	4	17	theme	myo-inositol	712:723	arg1	functions					699:707	The possible functions	686:707	The possible functions of myo-inositol in the mucilage droplets	686:748	The possible functions of myo-inositol in the mucilage droplets and the fate after being washed off from the leaf tentacles are proposed.
28940006	4	17	theme	myo-inositol	712:723	arg1	fate					758:761	the fate	754:761	the fate after being washed off from the leaf tentacles	754:808	The possible functions of myo-inositol in the mucilage droplets and the fate after being washed off from the leaf tentacles are proposed.
28940006	1	18	theme	carnivorous	180:190	arg1	capensis					206:213	the carnivorous plant Drosera capensis	176:213	the carnivorous plant Drosera capensis	176:213	The chemical composition of the exudate mucilage droplets of the carnivorous plant Drosera capensis was investigated using nuclear magnetic resonance spectroscopy.
28940006	7	19	theme	components	1219:1228	arg1	re-evaluation					1190:1202	A re-evaluation	1188:1202	A re-evaluation	1188:1202	A re-evaluation of the mineral components of the mucilage, reported 40 years ago, is presented from the viewpoints of the current result and plants' natural habitat.
28940006	6	20	theme	unique	1010:1015	arg1	property					1026:1033	the unique adhesive property	1006:1033	the unique adhesive property onto the hydrophobic insect body parts, as well as onto the nature's well-known superhydrophobic surfaces such as the leaves of the sacred lotus plants	1006:1185	These lipophilic moieties may provide the prey-trapping mucilage with the unique adhesive property onto the hydrophobic insect body parts, as well as onto the nature's well-known superhydrophobic surfaces such as the leaves of the sacred lotus plants.
28940006	3	21	theme	polysaccharide	569:582	arg1	component					584:592	polysaccharide component	569:592	polysaccharide component	569:592	It appears that myo-inositol escaped detection due to the commonly applied methodology on the chemical analysis of plant mucilage, such as dialysis, precipitation of polysaccharide component with alcohol, acid hydrolysis and detection of the resultant monosaccharide (aldose) units.
28940006	4	22	from	functions	699:707	arg1	droplets					741:748	the mucilage droplets	728:748	the mucilage droplets	728:748	The possible functions of myo-inositol in the mucilage droplets and the fate after being washed off from the leaf tentacles are proposed.
28940006	1	23	theme	plant	192:196	arg1	capensis					206:213	the carnivorous plant Drosera capensis	176:213	the carnivorous plant Drosera capensis	176:213	The chemical composition of the exudate mucilage droplets of the carnivorous plant Drosera capensis was investigated using nuclear magnetic resonance spectroscopy.
28940006	5	24	theme	polysaccharide	831:844	arg1	component					846:854	the polysaccharide component	827:854	the polysaccharide component	827:854	On the polysaccharide component, the presence of methyl ester and alkyl chain-like moieties could be confirmed.
28940006	2	25	theme	weight	343:348	arg1	polysaccharide					350:363	a very large molecular weight polysaccharide	320:363	beside a very large molecular weight polysaccharide a significant amount of myo-inositol	313:400	The mucilage was found to contain beside a very large molecular weight polysaccharide a significant amount of myo-inositol.
28940006	6	26	theme	superhydrophobic	1115:1130	arg1	surfaces					1132:1139	the nature's well-known superhydrophobic surfaces	1091:1139	the nature's well-known superhydrophobic surfaces such as the leaves of the sacred lotus plants	1091:1185	These lipophilic moieties may provide the prey-trapping mucilage with the unique adhesive property onto the hydrophobic insect body parts, as well as onto the nature's well-known superhydrophobic surfaces such as the leaves of the sacred lotus plants.
28940006	6	26	theme	superhydrophobic	1115:1130	arg1	the					1149:1151	the	1149:1151	the	1149:1151	These lipophilic moieties may provide the prey-trapping mucilage with the unique adhesive property onto the hydrophobic insect body parts, as well as onto the nature's well-known superhydrophobic surfaces such as the leaves of the sacred lotus plants.
28940006	1	27	theme	Drosera	198:204	arg1	capensis					206:213	the carnivorous plant Drosera capensis	176:213	the carnivorous plant Drosera capensis	176:213	The chemical composition of the exudate mucilage droplets of the carnivorous plant Drosera capensis was investigated using nuclear magnetic resonance spectroscopy.
28940006	6	28	dep	the	1149:1151	arg1	leaves					1153:1158	leaves	1153:1158	leaves	1153:1158	These lipophilic moieties may provide the prey-trapping mucilage with the unique adhesive property onto the hydrophobic insect body parts, as well as onto the nature's well-known superhydrophobic surfaces such as the leaves of the sacred lotus plants.
28940006	6	29	theme	adhesive	1017:1024	arg1	property					1026:1033	the unique adhesive property	1006:1033	the unique adhesive property onto the hydrophobic insect body parts, as well as onto the nature's well-known superhydrophobic surfaces such as the leaves of the sacred lotus plants	1006:1185	These lipophilic moieties may provide the prey-trapping mucilage with the unique adhesive property onto the hydrophobic insect body parts, as well as onto the nature's well-known superhydrophobic surfaces such as the leaves of the sacred lotus plants.
28940006	2	30	theme	molecular	333:341	arg1	polysaccharide					350:363	a very large molecular weight polysaccharide	320:363	beside a very large molecular weight polysaccharide a significant amount of myo-inositol	313:400	The mucilage was found to contain beside a very large molecular weight polysaccharide a significant amount of myo-inositol.
28940006	4	31	theme	leaf	795:798	arg1	tentacles					800:808	the leaf tentacles	791:808	the leaf tentacles	791:808	The possible functions of myo-inositol in the mucilage droplets and the fate after being washed off from the leaf tentacles are proposed.
28940006	1	32	theme	capensis	206:213	arg1	droplets					164:171	the exudate mucilage droplets	143:171	the exudate mucilage droplets of the carnivorous plant Drosera capensis	143:213	The chemical composition of the exudate mucilage droplets of the carnivorous plant Drosera capensis was investigated using nuclear magnetic resonance spectroscopy.
28940006	2	33	contain	contain	305:311	arg1	mucilage					283:290	The mucilage	279:290	The mucilage	279:290	The mucilage was found to contain beside a very large molecular weight polysaccharide a significant amount of myo-inositol.
28940006	2	33	contain	contain	305:311	arg2	beside					313:318	beside a very large molecular weight polysaccharide a significant amount of myo-inositol	313:400	beside a very large molecular weight polysaccharide a significant amount of myo-inositol	313:400	The mucilage was found to contain beside a very large molecular weight polysaccharide a significant amount of myo-inositol.
28940006	8	34	theme	findings	1457:1464	arg1	light					1440:1444	light	1440:1444	light of the new findings	1440:1464	A case for re-examination of the well-studied plant mucilaginous materials is made in light of the new findings.
28940006	8	35	theme	materials	1419:1427	arg1	re-examination					1365:1378	re-examination	1365:1378	re-examination of the well-studied plant mucilaginous materials	1365:1427	A case for re-examination of the well-studied plant mucilaginous materials is made in light of the new findings.
28940006	2	36	theme	large	327:331	arg1	polysaccharide					350:363	a very large molecular weight polysaccharide	320:363	beside a very large molecular weight polysaccharide a significant amount of myo-inositol	313:400	The mucilage was found to contain beside a very large molecular weight polysaccharide a significant amount of myo-inositol.
28940006	7	37	theme	result	1318:1323	arg1	viewpoints					1292:1301	the viewpoints	1288:1301	the viewpoints of the current result and plants' natural habitat	1288:1351	A re-evaluation of the mineral components of the mucilage, reported 40 years ago, is presented from the viewpoints of the current result and plants' natural habitat.
28940006	0	38	theme	myo-inositol	14:25	arg1	Occurrence					0:9	Occurrence	0:9	Occurrence of myo-inositol and alkyl-substituted polysaccharide in the prey-trapping mucilage of Drosera capensis.	0:113	Occurrence of myo-inositol and alkyl-substituted polysaccharide in the prey-trapping mucilage of Drosera capensis.
28940006	6	39	with	mucilage	992:999	arg1	property					1026:1033	the unique adhesive property	1006:1033	the unique adhesive property onto the hydrophobic insect body parts, as well as onto the nature's well-known superhydrophobic surfaces such as the leaves of the sacred lotus plants	1006:1185	These lipophilic moieties may provide the prey-trapping mucilage with the unique adhesive property onto the hydrophobic insect body parts, as well as onto the nature's well-known superhydrophobic surfaces such as the leaves of the sacred lotus plants.
28940006	8	40	theme	new	1453:1455	arg1	findings					1457:1464	the new findings	1449:1464	the new findings	1449:1464	A case for re-examination of the well-studied plant mucilaginous materials is made in light of the new findings.
28940006	1	41	theme	chemical	119:126	arg1	composition					128:138	The chemical composition	115:138	The chemical composition of the exudate mucilage droplets of the carnivorous plant Drosera capensis	115:213	The chemical composition of the exudate mucilage droplets of the carnivorous plant Drosera capensis was investigated using nuclear magnetic resonance spectroscopy.
28940006	5	42	theme	alkyl	890:894	arg1	moieties					907:914	alkyl chain-like moieties	890:914	alkyl chain-like moieties	890:914	On the polysaccharide component, the presence of methyl ester and alkyl chain-like moieties could be confirmed.
28940006	0	43	theme	polysaccharide	49:62	arg1	Occurrence					0:9	Occurrence	0:9	Occurrence of myo-inositol and alkyl-substituted polysaccharide in the prey-trapping mucilage of Drosera capensis.	0:113	Occurrence of myo-inositol and alkyl-substituted polysaccharide in the prey-trapping mucilage of Drosera capensis.
28940006	7	44	theme	current	1310:1316	arg1	result					1318:1323	the current result	1306:1323	the current result	1306:1323	A re-evaluation of the mineral components of the mucilage, reported 40 years ago, is presented from the viewpoints of the current result and plants' natural habitat.
28940006	2	45	dep	polysaccharide	350:363	arg1	myo-inositol					389:400	myo-inositol	389:400	myo-inositol	389:400	The mucilage was found to contain beside a very large molecular weight polysaccharide a significant amount of myo-inositol.
28940006	2	45	dep	polysaccharide	350:363	arg1	amount					379:384	a significant amount	365:384	beside a very large molecular weight polysaccharide a significant amount of myo-inositol	313:400	The mucilage was found to contain beside a very large molecular weight polysaccharide a significant amount of myo-inositol.
28940006	8	46	theme	mucilaginous	1406:1417	arg1	materials					1419:1427	the well-studied plant mucilaginous materials	1383:1427	the well-studied plant mucilaginous materials	1383:1427	A case for re-examination of the well-studied plant mucilaginous materials is made in light of the new findings.
28940006	3	47	theme	applied	470:476	arg1	methodology					478:488	the commonly applied methodology	457:488	the commonly applied methodology	457:488	It appears that myo-inositol escaped detection due to the commonly applied methodology on the chemical analysis of plant mucilage, such as dialysis, precipitation of polysaccharide component with alcohol, acid hydrolysis and detection of the resultant monosaccharide (aldose) units.
28940006	5	48	theme	methyl	873:878	arg1	ester					880:884	methyl ester	873:884	methyl ester	873:884	On the polysaccharide component, the presence of methyl ester and alkyl chain-like moieties could be confirmed.
28940006	0	49	theme	alkyl-substituted	31:47	arg1	polysaccharide					49:62	alkyl-substituted polysaccharide	31:62	alkyl-substituted polysaccharide	31:62	Occurrence of myo-inositol and alkyl-substituted polysaccharide in the prey-trapping mucilage of Drosera capensis.
28940006	5	50	theme	chain-like	896:905	arg1	moieties					907:914	alkyl chain-like moieties	890:914	alkyl chain-like moieties	890:914	On the polysaccharide component, the presence of methyl ester and alkyl chain-like moieties could be confirmed.
28940006	7	51	theme	natural	1337:1343	arg1	habitat					1345:1351	plants' natural habitat	1329:1351	plants' natural habitat	1329:1351	A re-evaluation of the mineral components of the mucilage, reported 40 years ago, is presented from the viewpoints of the current result and plants' natural habitat.
28940006	6	52	theme	insect	1056:1061	arg1	parts					1068:1072	the hydrophobic insect body parts	1040:1072	the hydrophobic insect body parts	1040:1072	These lipophilic moieties may provide the prey-trapping mucilage with the unique adhesive property onto the hydrophobic insect body parts, as well as onto the nature's well-known superhydrophobic surfaces such as the leaves of the sacred lotus plants.
28940006	5	53	theme	ester	880:884	arg1	presence					861:868	the presence	857:868	the presence of methyl ester and alkyl chain-like moieties	857:914	On the polysaccharide component, the presence of methyl ester and alkyl chain-like moieties could be confirmed.
28940006	1	54	theme	nuclear	238:244	arg1	resonance					255:263	nuclear magnetic resonance	238:263	nuclear magnetic resonance spectroscopy	238:276	The chemical composition of the exudate mucilage droplets of the carnivorous plant Drosera capensis was investigated using nuclear magnetic resonance spectroscopy.
28940006	6	55	theme	prey-trapping	978:990	arg1	mucilage					992:999	the prey-trapping mucilage	974:999	the prey-trapping mucilage with the unique adhesive property onto the hydrophobic insect body parts, as well as onto the nature's well-known superhydrophobic surfaces such as the leaves of the sacred lotus plants	974:1185	These lipophilic moieties may provide the prey-trapping mucilage with the unique adhesive property onto the hydrophobic insect body parts, as well as onto the nature's well-known superhydrophobic surfaces such as the leaves of the sacred lotus plants.
28940006	3	56	theme	units	679:683	arg1	dialysis					542:549	dialysis	542:549	dialysis	542:549	It appears that myo-inositol escaped detection due to the commonly applied methodology on the chemical analysis of plant mucilage, such as dialysis, precipitation of polysaccharide component with alcohol, acid hydrolysis and detection of the resultant monosaccharide (aldose) units.
28940006	3	56	theme	units	679:683	arg1	hydrolysis					613:622	acid hydrolysis	608:622	acid hydrolysis	608:622	It appears that myo-inositol escaped detection due to the commonly applied methodology on the chemical analysis of plant mucilage, such as dialysis, precipitation of polysaccharide component with alcohol, acid hydrolysis and detection of the resultant monosaccharide (aldose) units.
28940006	3	56	theme	units	679:683	arg1	detection					628:636	detection	628:636	detection of the resultant monosaccharide (aldose) units	628:683	It appears that myo-inositol escaped detection due to the commonly applied methodology on the chemical analysis of plant mucilage, such as dialysis, precipitation of polysaccharide component with alcohol, acid hydrolysis and detection of the resultant monosaccharide (aldose) units.
28940006	3	56	theme	units	679:683	arg1	precipitation					552:564	precipitation	552:564	precipitation of polysaccharide component with alcohol	552:605	It appears that myo-inositol escaped detection due to the commonly applied methodology on the chemical analysis of plant mucilage, such as dialysis, precipitation of polysaccharide component with alcohol, acid hydrolysis and detection of the resultant monosaccharide (aldose) units.
28940006	6	57	theme	lotus	1174:1178	arg1	plants					1180:1185	the sacred lotus plants	1163:1185	the sacred lotus plants	1163:1185	These lipophilic moieties may provide the prey-trapping mucilage with the unique adhesive property onto the hydrophobic insect body parts, as well as onto the nature's well-known superhydrophobic surfaces such as the leaves of the sacred lotus plants.
28940006	2	58	theme	significant	367:377	arg1	myo-inositol					389:400	myo-inositol	389:400	myo-inositol	389:400	The mucilage was found to contain beside a very large molecular weight polysaccharide a significant amount of myo-inositol.
28940006	2	58	theme	significant	367:377	arg1	amount					379:384	a significant amount	365:384	beside a very large molecular weight polysaccharide a significant amount of myo-inositol	313:400	The mucilage was found to contain beside a very large molecular weight polysaccharide a significant amount of myo-inositol.
28940006	3	59	theme	acid	608:611	arg1	hydrolysis					613:622	acid hydrolysis	608:622	acid hydrolysis	608:622	It appears that myo-inositol escaped detection due to the commonly applied methodology on the chemical analysis of plant mucilage, such as dialysis, precipitation of polysaccharide component with alcohol, acid hydrolysis and detection of the resultant monosaccharide (aldose) units.
28940006	5	60	theme	moieties	907:914	arg1	presence					861:868	the presence	857:868	the presence of methyl ester and alkyl chain-like moieties	857:914	On the polysaccharide component, the presence of methyl ester and alkyl chain-like moieties could be confirmed.
28940006	8	61	theme	plant	1400:1404	arg1	materials					1419:1427	the well-studied plant mucilaginous materials	1383:1427	the well-studied plant mucilaginous materials	1383:1427	A case for re-examination of the well-studied plant mucilaginous materials is made in light of the new findings.
28940006	3	62	theme	plant	518:522	arg1	mucilage					524:531	plant mucilage	518:531	plant mucilage	518:531	It appears that myo-inositol escaped detection due to the commonly applied methodology on the chemical analysis of plant mucilage, such as dialysis, precipitation of polysaccharide component with alcohol, acid hydrolysis and detection of the resultant monosaccharide (aldose) units.
28940006	4	63	from	fate	758:761	arg1	droplets					741:748	the mucilage droplets	728:748	the mucilage droplets	728:748	The possible functions of myo-inositol in the mucilage droplets and the fate after being washed off from the leaf tentacles are proposed.
28940006	1	64	theme	magnetic	246:253	arg1	resonance					255:263	nuclear magnetic resonance	238:263	nuclear magnetic resonance spectroscopy	238:276	The chemical composition of the exudate mucilage droplets of the carnivorous plant Drosera capensis was investigated using nuclear magnetic resonance spectroscopy.
28940006	3	65	theme	aldose	671:676	arg1	units					679:683	the resultant monosaccharide (aldose) units	641:683	the resultant monosaccharide (aldose) units	641:683	It appears that myo-inositol escaped detection due to the commonly applied methodology on the chemical analysis of plant mucilage, such as dialysis, precipitation of polysaccharide component with alcohol, acid hydrolysis and detection of the resultant monosaccharide (aldose) units.
28940006	1	66	theme	exudate	147:153	arg1	droplets					164:171	the exudate mucilage droplets	143:171	the exudate mucilage droplets of the carnivorous plant Drosera capensis	143:213	The chemical composition of the exudate mucilage droplets of the carnivorous plant Drosera capensis was investigated using nuclear magnetic resonance spectroscopy.
28940006	1	67	theme	resonance	255:263	arg1	spectroscopy					265:276	nuclear magnetic resonance spectroscopy	238:276	nuclear magnetic resonance spectroscopy	238:276	The chemical composition of the exudate mucilage droplets of the carnivorous plant Drosera capensis was investigated using nuclear magnetic resonance spectroscopy.
28940006	3	68	theme	chemical	497:504	arg1	analysis					506:513	the chemical analysis	493:513	the chemical analysis of plant mucilage	493:531	It appears that myo-inositol escaped detection due to the commonly applied methodology on the chemical analysis of plant mucilage, such as dialysis, precipitation of polysaccharide component with alcohol, acid hydrolysis and detection of the resultant monosaccharide (aldose) units.
28940006	3	69	theme	resultant	645:653	arg1	units					679:683	the resultant monosaccharide (aldose) units	641:683	the resultant monosaccharide (aldose) units	641:683	It appears that myo-inositol escaped detection due to the commonly applied methodology on the chemical analysis of plant mucilage, such as dialysis, precipitation of polysaccharide component with alcohol, acid hydrolysis and detection of the resultant monosaccharide (aldose) units.
28940006	1	70	theme	mucilage	155:162	arg1	droplets					164:171	the exudate mucilage droplets	143:171	the exudate mucilage droplets of the carnivorous plant Drosera capensis	143:213	The chemical composition of the exudate mucilage droplets of the carnivorous plant Drosera capensis was investigated using nuclear magnetic resonance spectroscopy.
28940006	4	71	theme	possible	690:697	arg1	functions					699:707	The possible functions	686:707	The possible functions of myo-inositol in the mucilage droplets	686:748	The possible functions of myo-inositol in the mucilage droplets and the fate after being washed off from the leaf tentacles are proposed.
28940006	6	72	theme	body	1063:1066	arg1	parts					1068:1072	the hydrophobic insect body parts	1040:1072	the hydrophobic insect body parts	1040:1072	These lipophilic moieties may provide the prey-trapping mucilage with the unique adhesive property onto the hydrophobic insect body parts, as well as onto the nature's well-known superhydrophobic surfaces such as the leaves of the sacred lotus plants.
28940006	0	73	theme	prey-trapping	71:83	arg1	mucilage					85:92	the prey-trapping mucilage	67:92	the prey-trapping mucilage of Drosera capensis	67:112	Occurrence of myo-inositol and alkyl-substituted polysaccharide in the prey-trapping mucilage of Drosera capensis.
28940006	7	74	theme	mineral	1211:1217	arg1	components					1219:1228	the mineral components	1207:1228	the mineral components of the mucilage	1207:1244	A re-evaluation of the mineral components of the mucilage, reported 40 years ago, is presented from the viewpoints of the current result and plants' natural habitat.
28940006	3	75	theme	monosaccharide	655:668	arg1	units					679:683	the resultant monosaccharide (aldose) units	641:683	the resultant monosaccharide (aldose) units	641:683	It appears that myo-inositol escaped detection due to the commonly applied methodology on the chemical analysis of plant mucilage, such as dialysis, precipitation of polysaccharide component with alcohol, acid hydrolysis and detection of the resultant monosaccharide (aldose) units.
28940006	1	76	theme	droplets	164:171	arg1	composition					128:138	The chemical composition	115:138	The chemical composition of the exudate mucilage droplets of the carnivorous plant Drosera capensis	115:213	The chemical composition of the exudate mucilage droplets of the carnivorous plant Drosera capensis was investigated using nuclear magnetic resonance spectroscopy.
28940006	3	77	with	hydrolysis	613:622	arg1	alcohol					599:605	alcohol	599:605	alcohol	599:605	It appears that myo-inositol escaped detection due to the commonly applied methodology on the chemical analysis of plant mucilage, such as dialysis, precipitation of polysaccharide component with alcohol, acid hydrolysis and detection of the resultant monosaccharide (aldose) units.
28940006	3	78	with	detection	628:636	arg1	alcohol					599:605	alcohol	599:605	alcohol	599:605	It appears that myo-inositol escaped detection due to the commonly applied methodology on the chemical analysis of plant mucilage, such as dialysis, precipitation of polysaccharide component with alcohol, acid hydrolysis and detection of the resultant monosaccharide (aldose) units.
27481294	7	0	theme	method	966:971	arg1	use					906:908	the use	902:908	the use of the needle trap gas chromatography-mass spectrometry method	902:971	With the use of the needle trap gas chromatography-mass spectrometry method, we found that there are significant differences in the composition of the volatile aroma and flavor compounds secreted by the mycelia cultivated in different media.
27481294	4	1	theme	selenium	516:523	arg1	concentrations					525:538	The total selenium concentrations	506:538	The total selenium concentrations in the mycelium biomass and in the isolated crude polysaccharides	506:604	The total selenium concentrations in the mycelium biomass and in the isolated crude polysaccharides were determined using atomic absorption spectroscopy.
27481294	0	2	theme	Chemopreventive	68:82	arg1	Preparations					84:95	Mushroom-Derived Chemopreventive Preparations	51:95	Mushroom-Derived Chemopreventive Preparations	51:95	A Search for the Optimum Selenium Source to Obtain Mushroom-Derived Chemopreventive Preparations.
27481294	7	3	theme	gas	929:931	arg1	spectrometry					953:964	needle trap gas chromatography-mass spectrometry	917:964	the needle trap gas chromatography-mass spectrometry method	913:971	With the use of the needle trap gas chromatography-mass spectrometry method, we found that there are significant differences in the composition of the volatile aroma and flavor compounds secreted by the mycelia cultivated in different media.
27481294	4	4	theme	total	510:514	arg1	concentrations					525:538	The total selenium concentrations	506:538	The total selenium concentrations in the mycelium biomass and in the isolated crude polysaccharides	506:604	The total selenium concentrations in the mycelium biomass and in the isolated crude polysaccharides were determined using atomic absorption spectroscopy.
27481294	6	5	theme	Se-yeast-enriched	872:888	arg1	media					890:894	Se-yeast-enriched media	872:894	Se-yeast-enriched media	872:894	A higher concentration of selenium was also found in the crude polysaccharide fractions isolated from the mycelium grown in Se-yeast-enriched media.
27481294	7	6	theme	needle	917:922	arg1	spectrometry					953:964	needle trap gas chromatography-mass spectrometry	917:964	the needle trap gas chromatography-mass spectrometry method	913:971	With the use of the needle trap gas chromatography-mass spectrometry method, we found that there are significant differences in the composition of the volatile aroma and flavor compounds secreted by the mycelia cultivated in different media.
27481294	4	7	from	concentrations	525:538	arg1	biomass					556:562	the mycelium biomass	543:562	the mycelium biomass	543:562	The total selenium concentrations in the mycelium biomass and in the isolated crude polysaccharides were determined using atomic absorption spectroscopy.
27481294	4	7	from	concentrations	525:538	arg1	polysaccharides					590:604	the isolated crude polysaccharides	571:604	the isolated crude polysaccharides	571:604	The total selenium concentrations in the mycelium biomass and in the isolated crude polysaccharides were determined using atomic absorption spectroscopy.
27481294	6	8	theme	selenium	774:781	arg1	concentration					757:769	A higher concentration	748:769	A higher concentration of selenium	748:781	A higher concentration of selenium was also found in the crude polysaccharide fractions isolated from the mycelium grown in Se-yeast-enriched media.
27481294	3	9	dep	added	384:388	arg1	Sel-Plex					466:473	Sel-Plex	466:473	Sel-Plex	466:473	Selenium was added to the medium either in the form of sodium selenite or in form of Se-yeast (Sel-Plex; Alltech Inc., Lexington, KY).
27481294	7	10	theme	trap	924:927	arg1	spectrometry					953:964	needle trap gas chromatography-mass spectrometry	917:964	the needle trap gas chromatography-mass spectrometry method	913:971	With the use of the needle trap gas chromatography-mass spectrometry method, we found that there are significant differences in the composition of the volatile aroma and flavor compounds secreted by the mycelia cultivated in different media.
27481294	7	11	theme	flavor	1067:1072	arg1	compounds					1074:1082	the volatile aroma and flavor compounds	1044:1082	compounds	1074:1082	With the use of the needle trap gas chromatography-mass spectrometry method, we found that there are significant differences in the composition of the volatile aroma and flavor compounds secreted by the mycelia cultivated in different media.
27481294	7	12	theme	compounds	1074:1082	arg1	composition					1029:1039	the composition	1025:1039	the composition of the volatile aroma and flavor compounds secreted by the mycelia cultivated in different media	1025:1136	With the use of the needle trap gas chromatography-mass spectrometry method, we found that there are significant differences in the composition of the volatile aroma and flavor compounds secreted by the mycelia cultivated in different media.
27481294	7	13	from	differences	1010:1020	arg1	composition					1029:1039	the composition	1025:1039	the composition of the volatile aroma and flavor compounds secreted by the mycelia cultivated in different media	1025:1136	With the use of the needle trap gas chromatography-mass spectrometry method, we found that there are significant differences in the composition of the volatile aroma and flavor compounds secreted by the mycelia cultivated in different media.
27481294	7	14	theme	aroma	1057:1061	arg1	composition					1029:1039	the composition	1025:1039	the composition of the volatile aroma and flavor compounds secreted by the mycelia cultivated in different media	1025:1136	With the use of the needle trap gas chromatography-mass spectrometry method, we found that there are significant differences in the composition of the volatile aroma and flavor compounds secreted by the mycelia cultivated in different media.
27481294	6	15	theme	higher	750:755	arg1	concentration					757:769	A higher concentration	748:769	A higher concentration of selenium	748:781	A higher concentration of selenium was also found in the crude polysaccharide fractions isolated from the mycelium grown in Se-yeast-enriched media.
27481294	1	16	theme	immunoactive	259:270	arg1	polysaccharides					282:296	immunoactive cell wall polysaccharides	259:296	immunoactive cell wall polysaccharides	259:296	The objective of this research was to test whether selenium-yeast (Se-yeast) is a better source of selenium than sodium selenite for accumulation in mycelia and immunoactive cell wall polysaccharides.
27481294	7	17	theme	different	1122:1130	arg1	media					1132:1136	different media	1122:1136	different media	1122:1136	With the use of the needle trap gas chromatography-mass spectrometry method, we found that there are significant differences in the composition of the volatile aroma and flavor compounds secreted by the mycelia cultivated in different media.
27481294	3	18	dep	Sel-Plex	466:473	arg1	Lexington					490:498	Lexington	490:498	Lexington	490:498	Selenium was added to the medium either in the form of sodium selenite or in form of Se-yeast (Sel-Plex; Alltech Inc., Lexington, KY).
27481294	2	19	theme	µg/mL	364:368	arg1	concentration					344:356	a concentration	342:356	a concentration of 20 µg/mL	342:368	Culture media were enriched in selenium to a concentration of 20 µg/mL.
27481294	1	20	theme	cell	272:275	arg1	polysaccharides					282:296	immunoactive cell wall polysaccharides	259:296	immunoactive cell wall polysaccharides	259:296	The objective of this research was to test whether selenium-yeast (Se-yeast) is a better source of selenium than sodium selenite for accumulation in mycelia and immunoactive cell wall polysaccharides.
27481294	2	21	theme	Culture	299:305	arg1	media					307:311	Culture media	299:311	Culture media	299:311	Culture media were enriched in selenium to a concentration of 20 µg/mL.
27481294	3	22	theme	selenite	433:440	arg1	form					418:421	the form	414:421	the form of sodium selenite	414:440	Selenium was added to the medium either in the form of sodium selenite or in form of Se-yeast (Sel-Plex; Alltech Inc., Lexington, KY).
27481294	1	23	theme	wall	277:280	arg1	polysaccharides					282:296	immunoactive cell wall polysaccharides	259:296	immunoactive cell wall polysaccharides	259:296	The objective of this research was to test whether selenium-yeast (Se-yeast) is a better source of selenium than sodium selenite for accumulation in mycelia and immunoactive cell wall polysaccharides.
27481294	7	24	theme	volatile	1048:1055	arg1	aroma					1057:1061	the volatile aroma and flavor compounds	1044:1082	aroma	1057:1061	With the use of the needle trap gas chromatography-mass spectrometry method, we found that there are significant differences in the composition of the volatile aroma and flavor compounds secreted by the mycelia cultivated in different media.
27481294	6	25	attach	isolated	836:843	arg2	fractions					826:834	the crude polysaccharide fractions	801:834	the crude polysaccharide fractions isolated from the mycelium grown in Se-yeast-enriched media	801:894	A higher concentration of selenium was also found in the crude polysaccharide fractions isolated from the mycelium grown in Se-yeast-enriched media.
27481294	6	25	attach	isolated	836:843	arg1	mycelium					854:861	the mycelium	850:861	the mycelium grown in Se-yeast-enriched media	850:894	A higher concentration of selenium was also found in the crude polysaccharide fractions isolated from the mycelium grown in Se-yeast-enriched media.
27481294	0	26	theme	Selenium	25:32	arg1	Source					34:39	the Optimum Selenium Source	13:39	the Optimum Selenium Source	13:39	A Search for the Optimum Selenium Source to Obtain Mushroom-Derived Chemopreventive Preparations.
27481294	3	27	theme	Se-yeast	456:463	arg1	form					448:451	form	448:451	form of Se-yeast	448:463	Selenium was added to the medium either in the form of sodium selenite or in form of Se-yeast (Sel-Plex; Alltech Inc., Lexington, KY).
27481294	1	28	from	accumulation	231:242	arg1	polysaccharides					282:296	immunoactive cell wall polysaccharides	259:296	immunoactive cell wall polysaccharides	259:296	The objective of this research was to test whether selenium-yeast (Se-yeast) is a better source of selenium than sodium selenite for accumulation in mycelia and immunoactive cell wall polysaccharides.
27481294	1	28	from	accumulation	231:242	arg1	mycelia					247:253	mycelia	247:253	mycelia	247:253	The objective of this research was to test whether selenium-yeast (Se-yeast) is a better source of selenium than sodium selenite for accumulation in mycelia and immunoactive cell wall polysaccharides.
27481294	4	29	theme	isolated	575:582	arg1	polysaccharides					590:604	the isolated crude polysaccharides	571:604	the isolated crude polysaccharides	571:604	The total selenium concentrations in the mycelium biomass and in the isolated crude polysaccharides were determined using atomic absorption spectroscopy.
27481294	4	30	theme	crude	584:588	arg1	polysaccharides					590:604	the isolated crude polysaccharides	571:604	the isolated crude polysaccharides	571:604	The total selenium concentrations in the mycelium biomass and in the isolated crude polysaccharides were determined using atomic absorption spectroscopy.
27481294	0	31	theme	Optimum	17:23	arg1	Source					34:39	the Optimum Selenium Source	13:39	the Optimum Selenium Source	13:39	A Search for the Optimum Selenium Source to Obtain Mushroom-Derived Chemopreventive Preparations.
27481294	3	32	theme	sodium	426:431	arg1	selenite					433:440	sodium selenite	426:440	sodium selenite	426:440	Selenium was added to the medium either in the form of sodium selenite or in form of Se-yeast (Sel-Plex; Alltech Inc., Lexington, KY).
27481294	6	33	theme	polysaccharide	811:824	arg1	fractions					826:834	the crude polysaccharide fractions	801:834	the crude polysaccharide fractions isolated from the mycelium grown in Se-yeast-enriched media	801:894	A higher concentration of selenium was also found in the crude polysaccharide fractions isolated from the mycelium grown in Se-yeast-enriched media.
27481294	6	34	theme	crude	805:809	arg1	fractions					826:834	the crude polysaccharide fractions	801:834	the crude polysaccharide fractions isolated from the mycelium grown in Se-yeast-enriched media	801:894	A higher concentration of selenium was also found in the crude polysaccharide fractions isolated from the mycelium grown in Se-yeast-enriched media.
27481294	1	35	theme	research	120:127	arg1	objective					102:110	The objective	98:110	The objective of this research	98:127	The objective of this research was to test whether selenium-yeast (Se-yeast) is a better source of selenium than sodium selenite for accumulation in mycelia and immunoactive cell wall polysaccharides.
27481294	7	36	theme	significant	998:1008	arg1	differences					1010:1020	significant differences	998:1020	significant differences in the composition of the volatile aroma and flavor compounds secreted by the mycelia cultivated in different media	998:1136	With the use of the needle trap gas chromatography-mass spectrometry method, we found that there are significant differences in the composition of the volatile aroma and flavor compounds secreted by the mycelia cultivated in different media.
27481294	7	37	theme	chromatography-mass	933:951	arg1	spectrometry					953:964	needle trap gas chromatography-mass spectrometry	917:964	the needle trap gas chromatography-mass spectrometry method	913:971	With the use of the needle trap gas chromatography-mass spectrometry method, we found that there are significant differences in the composition of the volatile aroma and flavor compounds secreted by the mycelia cultivated in different media.
27481294	6	38	located	found	792:796	arg1	fractions					826:834	the crude polysaccharide fractions	801:834	the crude polysaccharide fractions isolated from the mycelium grown in Se-yeast-enriched media	801:894	A higher concentration of selenium was also found in the crude polysaccharide fractions isolated from the mycelium grown in Se-yeast-enriched media.
27481294	6	38	located	found	792:796	arg2	concentration					757:769	A higher concentration	748:769	A higher concentration of selenium	748:781	A higher concentration of selenium was also found in the crude polysaccharide fractions isolated from the mycelium grown in Se-yeast-enriched media.
27481294	4	39	theme	mycelium	547:554	arg1	biomass					556:562	the mycelium biomass	543:562	the mycelium biomass	543:562	The total selenium concentrations in the mycelium biomass and in the isolated crude polysaccharides were determined using atomic absorption spectroscopy.
27481294	1	40	theme	sodium	211:216	arg1	selenite					218:225	sodium selenite	211:225	sodium selenite for accumulation in mycelia and immunoactive cell wall polysaccharides	211:296	The objective of this research was to test whether selenium-yeast (Se-yeast) is a better source of selenium than sodium selenite for accumulation in mycelia and immunoactive cell wall polysaccharides.
27481294	0	41	theme	Mushroom-Derived	51:66	arg1	Preparations					84:95	Mushroom-Derived Chemopreventive Preparations	51:95	Mushroom-Derived Chemopreventive Preparations	51:95	A Search for the Optimum Selenium Source to Obtain Mushroom-Derived Chemopreventive Preparations.
27481294	1	42	theme	selenium	197:204	arg1	source					187:192	a better source	178:192	a better source of selenium than sodium selenite for accumulation in mycelia and immunoactive cell wall polysaccharides	178:296	The objective of this research was to test whether selenium-yeast (Se-yeast) is a better source of selenium than sodium selenite for accumulation in mycelia and immunoactive cell wall polysaccharides.
27481294	1	42	theme	selenium	197:204	arg1	selenium-yeast					149:162	selenium-yeast	149:162	selenium-yeast (Se-yeast)	149:173	The objective of this research was to test whether selenium-yeast (Se-yeast) is a better source of selenium than sodium selenite for accumulation in mycelia and immunoactive cell wall polysaccharides.
27481294	1	43	theme	better	180:185	arg1	source					187:192	a better source	178:192	a better source of selenium than sodium selenite for accumulation in mycelia and immunoactive cell wall polysaccharides	178:296	The objective of this research was to test whether selenium-yeast (Se-yeast) is a better source of selenium than sodium selenite for accumulation in mycelia and immunoactive cell wall polysaccharides.
27481294	1	43	theme	better	180:185	arg1	selenium-yeast					149:162	selenium-yeast	149:162	selenium-yeast (Se-yeast)	149:173	The objective of this research was to test whether selenium-yeast (Se-yeast) is a better source of selenium than sodium selenite for accumulation in mycelia and immunoactive cell wall polysaccharides.
27481294	4	44	theme	absorption	635:644	arg1	spectroscopy					646:657	atomic absorption spectroscopy	628:657	atomic absorption spectroscopy	628:657	The total selenium concentrations in the mycelium biomass and in the isolated crude polysaccharides were determined using atomic absorption spectroscopy.
27481294	7	45	theme	spectrometry	953:964	arg1	method					966:971	the needle trap gas chromatography-mass spectrometry method	913:971	the needle trap gas chromatography-mass spectrometry method	913:971	With the use of the needle trap gas chromatography-mass spectrometry method, we found that there are significant differences in the composition of the volatile aroma and flavor compounds secreted by the mycelia cultivated in different media.
27481294	4	46	theme	atomic	628:633	arg1	spectroscopy					646:657	atomic absorption spectroscopy	628:657	atomic absorption spectroscopy	628:657	The total selenium concentrations in the mycelium biomass and in the isolated crude polysaccharides were determined using atomic absorption spectroscopy.
27550933	6	0	theme	Chemical	1144:1151	arg1	analyses					1153:1160	Chemical analyses	1144:1160	Chemical analyses	1144:1160	Chemical analyses showed no difference in the composition as well as the linkage of 19A subtype capsular saccharides grown in defined and undefined growth media, which is consistent with a trisaccharide repeat unit composed of rhamnose, N-acetyl-mannosamine, and glucose.
27550933	8	1	theme	capsule	1663:1669	arg1	variant					1681:1687	a capsule structure variant	1661:1687	a capsule structure variant	1661:1687	The serotype 19A subtype shift observed after the introduction of the PCV7 can therefore not be explained by selection of a capsule structure variant.
27550933	1	2	theme	pneumococcal	266:277	arg1	PCV7					298:301	PCV7	298:301	PCV7	298:301	Serotype 19A strains have emerged as a cause of invasive pneumococcal disease after the introduction of the 7-valent pneumococcal conjugate vaccine (PCV7), and serotype 19A has now been included in the recent 13-valent vaccine (PCV13).
27550933	1	2	theme	pneumococcal	266:277	arg1	vaccine					289:295	the 7-valent pneumococcal conjugate vaccine	253:295	the 7-valent pneumococcal conjugate vaccine (PCV7)	253:302	Serotype 19A strains have emerged as a cause of invasive pneumococcal disease after the introduction of the 7-valent pneumococcal conjugate vaccine (PCV7), and serotype 19A has now been included in the recent 13-valent vaccine (PCV13).
27550933	7	3	theme	subtype	1523:1529	arg1	variance					1466:1473	no structural variance	1452:1473	no structural variance dependent of the nutritional environment or the subtype	1452:1529	In summary, our study suggests that no structural variance dependent of the nutritional environment or the subtype exists.
27550933	4	4	theme	chromatography-mass	937:955	arg1	GC-MS					971:975	GC-MS	971:975	GC-MS	971:975	We therefore analyzed the distribution of 19A subtypes collected within a Swiss national surveillance program and determined capsule composition under different nutritional conditions with high-performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS), and nuclear magnetic resonance (NMR) spectroscopy.
27550933	4	4	theme	chromatography-mass	937:955	arg1	spectrometry					957:968	gas chromatography-mass spectrometry	933:968	gas chromatography-mass spectrometry (GC-MS)	933:976	We therefore analyzed the distribution of 19A subtypes collected within a Swiss national surveillance program and determined capsule composition under different nutritional conditions with high-performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS), and nuclear magnetic resonance (NMR) spectroscopy.
27550933	6	5	theme	19A	1228:1230	arg1	saccharides					1249:1259	19A subtype capsular saccharides	1228:1259	19A subtype capsular saccharides grown in defined and undefined growth media, which is consistent with a trisaccharide repeat unit composed of rhamnose, N-acetyl-mannosamine, and glucose	1228:1413	Chemical analyses showed no difference in the composition as well as the linkage of 19A subtype capsular saccharides grown in defined and undefined growth media, which is consistent with a trisaccharide repeat unit composed of rhamnose, N-acetyl-mannosamine, and glucose.
27550933	8	6	theme	variant	1681:1687	arg1	selection					1648:1656	selection	1648:1656	selection of a capsule structure variant	1648:1687	The serotype 19A subtype shift observed after the introduction of the PCV7 can therefore not be explained by selection of a capsule structure variant.
27550933	3	7	from	differences	659:669	arg1	capsules					687:694	serotype 19A capsules	674:694	serotype 19A capsules	674:694	Pneumococcal vaccine effectiveness and serotyping accuracy might be impaired by structural differences in serotype 19A capsules.
27550933	1	8	theme	vaccine	289:295	arg1	introduction					237:248	the introduction	233:248	the introduction of the 7-valent pneumococcal conjugate vaccine (PCV7)	233:302	Serotype 19A strains have emerged as a cause of invasive pneumococcal disease after the introduction of the 7-valent pneumococcal conjugate vaccine (PCV7), and serotype 19A has now been included in the recent 13-valent vaccine (PCV13).
27550933	9	9	theme	composition	1707:1717	arg1	analysis					1719:1726	capsule composition analysis	1699:1726	capsule composition analysis of emerging 19A clones	1699:1749	However, capsule composition analysis of emerging 19A clones is recommended in cases where there is no other explanation for a selective advantage, such as antibiotic resistance or loss or acquisition of other virulence factors.
27550933	7	10	theme	structural	1455:1464	arg1	variance					1466:1473	no structural variance	1452:1473	no structural variance dependent of the nutritional environment or the subtype	1452:1529	In summary, our study suggests that no structural variance dependent of the nutritional environment or the subtype exists.
27550933	3	11	dep	19A	683:685	arg1	serotype					674:681	serotype	674:681	serotype	674:681	Pneumococcal vaccine effectiveness and serotyping accuracy might be impaired by structural differences in serotype 19A capsules.
27550933	7	12	theme	nutritional	1492:1502	arg1	environment					1504:1514	the nutritional environment	1488:1514	the nutritional environment	1488:1514	In summary, our study suggests that no structural variance dependent of the nutritional environment or the subtype exists.
27550933	6	13	theme	growth	1292:1297	arg1	media					1299:1303	defined and undefined growth media	1270:1303	defined and undefined growth media	1270:1303	Chemical analyses showed no difference in the composition as well as the linkage of 19A subtype capsular saccharides grown in defined and undefined growth media, which is consistent with a trisaccharide repeat unit composed of rhamnose, N-acetyl-mannosamine, and glucose.
27550933	3	14	theme	vaccine	581:587	arg1	effectiveness					589:601	Pneumococcal vaccine effectiveness	568:601	Pneumococcal vaccine effectiveness	568:601	Pneumococcal vaccine effectiveness and serotyping accuracy might be impaired by structural differences in serotype 19A capsules.
27550933	9	15	theme	19A	1740:1742	arg1	clones					1744:1749	19A clones	1740:1749	19A clones	1740:1749	However, capsule composition analysis of emerging 19A clones is recommended in cases where there is no other explanation for a selective advantage, such as antibiotic resistance or loss or acquisition of other virulence factors.
27550933	0	16	theme	Nutritional	124:134	arg1	Environment					136:146	the Nutritional Environment	120:146	the Nutritional Environment	120:146	Polysaccharide Capsule Composition of Pneumococcal Serotype 19A Subtypes Is Unaltered among Subtypes and Independent of the Nutritional Environment.
27550933	1	17	theme	pneumococcal	206:217	arg1	disease					219:225	invasive pneumococcal disease	197:225	invasive pneumococcal disease	197:225	Serotype 19A strains have emerged as a cause of invasive pneumococcal disease after the introduction of the 7-valent pneumococcal conjugate vaccine (PCV7), and serotype 19A has now been included in the recent 13-valent vaccine (PCV13).
27550933	4	18	theme	magnetic	991:998	arg1	NMR					1011:1013	NMR	1011:1013	NMR	1011:1013	We therefore analyzed the distribution of 19A subtypes collected within a Swiss national surveillance program and determined capsule composition under different nutritional conditions with high-performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS), and nuclear magnetic resonance (NMR) spectroscopy.
27550933	4	18	theme	magnetic	991:998	arg1	resonance					1000:1008	nuclear magnetic resonance	983:1008	nuclear magnetic resonance (NMR) spectroscopy	983:1027	We therefore analyzed the distribution of 19A subtypes collected within a Swiss national surveillance program and determined capsule composition under different nutritional conditions with high-performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS), and nuclear magnetic resonance (NMR) spectroscopy.
27550933	6	19	theme	undefined	1282:1290	arg1	media					1299:1303	defined and undefined growth media	1270:1303	defined and undefined growth media	1270:1303	Chemical analyses showed no difference in the composition as well as the linkage of 19A subtype capsular saccharides grown in defined and undefined growth media, which is consistent with a trisaccharide repeat unit composed of rhamnose, N-acetyl-mannosamine, and glucose.
27550933	8	20	dep	19A	1552:1554	arg1	serotype					1543:1550	serotype	1543:1550	serotype	1543:1550	The serotype 19A subtype shift observed after the introduction of the PCV7 can therefore not be explained by selection of a capsule structure variant.
27550933	5	21	theme	19A-I	1128:1132	arg1	decrease					1116:1123	decrease	1116:1123	decrease of 19A-I	1116:1132	After the introduction of PCV7, a significant relative increase of subtype 19A-II and decrease of 19A-I occurred.
27550933	5	21	theme	19A-I	1128:1132	arg1	increase					1085:1092	a significant relative increase	1062:1092	a significant relative increase of subtype 19A-II	1062:1110	After the introduction of PCV7, a significant relative increase of subtype 19A-II and decrease of 19A-I occurred.
27550933	6	22	theme	defined	1270:1276	arg1	media					1299:1303	defined and undefined growth media	1270:1303	defined and undefined growth media	1270:1303	Chemical analyses showed no difference in the composition as well as the linkage of 19A subtype capsular saccharides grown in defined and undefined growth media, which is consistent with a trisaccharide repeat unit composed of rhamnose, N-acetyl-mannosamine, and glucose.
27550933	4	23	theme	19A	739:741	arg1	subtypes					743:750	19A subtypes	739:750	19A subtypes collected within a Swiss national surveillance program	739:805	We therefore analyzed the distribution of 19A subtypes collected within a Swiss national surveillance program and determined capsule composition under different nutritional conditions with high-performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS), and nuclear magnetic resonance (NMR) spectroscopy.
27550933	4	24	theme	national	777:784	arg1	program					799:805	a Swiss national surveillance program	769:805	a Swiss national surveillance program	769:805	We therefore analyzed the distribution of 19A subtypes collected within a Swiss national surveillance program and determined capsule composition under different nutritional conditions with high-performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS), and nuclear magnetic resonance (NMR) spectroscopy.
27550933	6	25	theme	trisaccharide	1333:1345	arg1	unit					1354:1357	a trisaccharide repeat unit	1331:1357	a trisaccharide repeat unit composed of rhamnose, N-acetyl-mannosamine, and glucose	1331:1413	Chemical analyses showed no difference in the composition as well as the linkage of 19A subtype capsular saccharides grown in defined and undefined growth media, which is consistent with a trisaccharide repeat unit composed of rhamnose, N-acetyl-mannosamine, and glucose.
27550933	5	26	theme	19A-II	1105:1110	arg1	decrease					1116:1123	decrease	1116:1123	decrease of 19A-I	1116:1132	After the introduction of PCV7, a significant relative increase of subtype 19A-II and decrease of 19A-I occurred.
27550933	5	26	theme	19A-II	1105:1110	arg1	increase					1085:1092	a significant relative increase	1062:1092	a significant relative increase of subtype 19A-II	1062:1110	After the introduction of PCV7, a significant relative increase of subtype 19A-II and decrease of 19A-I occurred.
27550933	7	27	theme	dependent	1475:1483	arg1	variance					1466:1473	no structural variance	1452:1473	no structural variance dependent of the nutritional environment or the subtype	1452:1529	In summary, our study suggests that no structural variance dependent of the nutritional environment or the subtype exists.
27550933	2	28	theme	environment-dependent	488:508	arg1	variation					510:518	nutritional environment-dependent variation	476:518	nutritional environment-dependent variation of the 19A capsule structure	476:547	Genetic analysis has revealed at least three different capsular serotype 19A subtypes, and nutritional environment-dependent variation of the 19A capsule structure has been reported.
27550933	6	29	theme	capsular	1240:1247	arg1	saccharides					1249:1259	19A subtype capsular saccharides	1228:1259	19A subtype capsular saccharides grown in defined and undefined growth media, which is consistent with a trisaccharide repeat unit composed of rhamnose, N-acetyl-mannosamine, and glucose	1228:1413	Chemical analyses showed no difference in the composition as well as the linkage of 19A subtype capsular saccharides grown in defined and undefined growth media, which is consistent with a trisaccharide repeat unit composed of rhamnose, N-acetyl-mannosamine, and glucose.
27550933	4	30	with	conditions	870:879	arg1	GC-MS					971:975	GC-MS	971:975	GC-MS	971:975	We therefore analyzed the distribution of 19A subtypes collected within a Swiss national surveillance program and determined capsule composition under different nutritional conditions with high-performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS), and nuclear magnetic resonance (NMR) spectroscopy.
27550933	4	30	with	conditions	870:879	arg1	spectrometry					957:968	gas chromatography-mass spectrometry	933:968	gas chromatography-mass spectrometry (GC-MS)	933:976	We therefore analyzed the distribution of 19A subtypes collected within a Swiss national surveillance program and determined capsule composition under different nutritional conditions with high-performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS), and nuclear magnetic resonance (NMR) spectroscopy.
27550933	4	30	with	conditions	870:879	arg1	HPLC					926:929	HPLC	926:929	HPLC	926:929	We therefore analyzed the distribution of 19A subtypes collected within a Swiss national surveillance program and determined capsule composition under different nutritional conditions with high-performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS), and nuclear magnetic resonance (NMR) spectroscopy.
27550933	4	30	with	conditions	870:879	arg1	chromatography					910:923	high-performance liquid chromatography	886:923	high-performance liquid chromatography (HPLC)	886:930	We therefore analyzed the distribution of 19A subtypes collected within a Swiss national surveillance program and determined capsule composition under different nutritional conditions with high-performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS), and nuclear magnetic resonance (NMR) spectroscopy.
27550933	4	30	with	conditions	870:879	arg1	spectroscopy					1016:1027	nuclear magnetic resonance (NMR) spectroscopy	983:1027	nuclear magnetic resonance (NMR) spectroscopy	983:1027	We therefore analyzed the distribution of 19A subtypes collected within a Swiss national surveillance program and determined capsule composition under different nutritional conditions with high-performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS), and nuclear magnetic resonance (NMR) spectroscopy.
27550933	9	31	theme	other	1793:1797	arg1	explanation					1799:1809	no other explanation	1790:1809	no other explanation for a selective advantage	1790:1835	However, capsule composition analysis of emerging 19A clones is recommended in cases where there is no other explanation for a selective advantage, such as antibiotic resistance or loss or acquisition of other virulence factors.
27550933	9	32	theme	capsule	1699:1705	arg1	analysis					1719:1726	capsule composition analysis	1699:1726	capsule composition analysis of emerging 19A clones	1699:1749	However, capsule composition analysis of emerging 19A clones is recommended in cases where there is no other explanation for a selective advantage, such as antibiotic resistance or loss or acquisition of other virulence factors.
27550933	2	33	theme	19A	527:529	arg1	structure					539:547	the 19A capsule structure	523:547	the 19A capsule structure	523:547	Genetic analysis has revealed at least three different capsular serotype 19A subtypes, and nutritional environment-dependent variation of the 19A capsule structure has been reported.
27550933	8	34	theme	19A	1552:1554	arg1	shift					1564:1568	The serotype 19A subtype shift	1539:1568	The serotype 19A subtype shift observed after the introduction of the PCV7	1539:1612	The serotype 19A subtype shift observed after the introduction of the PCV7 can therefore not be explained by selection of a capsule structure variant.
27550933	2	35	dep	19A	458:460	arg1	serotype					449:456	capsular serotype	440:456	capsular serotype	440:456	Genetic analysis has revealed at least three different capsular serotype 19A subtypes, and nutritional environment-dependent variation of the 19A capsule structure has been reported.
27550933	9	36	theme	selective	1817:1825	arg1	advantage					1827:1835	a selective advantage	1815:1835	a selective advantage	1815:1835	However, capsule composition analysis of emerging 19A clones is recommended in cases where there is no other explanation for a selective advantage, such as antibiotic resistance or loss or acquisition of other virulence factors.
27550933	1	37	theme	13-valent	358:366	arg1	PCV13					377:381	PCV13	377:381	PCV13	377:381	Serotype 19A strains have emerged as a cause of invasive pneumococcal disease after the introduction of the 7-valent pneumococcal conjugate vaccine (PCV7), and serotype 19A has now been included in the recent 13-valent vaccine (PCV13).
27550933	1	37	theme	13-valent	358:366	arg1	vaccine					368:374	the recent 13-valent vaccine	347:374	the recent 13-valent vaccine (PCV13)	347:382	Serotype 19A strains have emerged as a cause of invasive pneumococcal disease after the introduction of the 7-valent pneumococcal conjugate vaccine (PCV7), and serotype 19A has now been included in the recent 13-valent vaccine (PCV13).
27550933	2	38	theme	different	430:438	arg1	subtypes					462:469	at least three different capsular serotype 19A subtypes	415:469	at least three different capsular serotype 19A subtypes	415:469	Genetic analysis has revealed at least three different capsular serotype 19A subtypes, and nutritional environment-dependent variation of the 19A capsule structure has been reported.
27550933	0	39	theme	Polysaccharide	0:13	arg1	Composition					23:33	Polysaccharide Capsule Composition	0:33	Polysaccharide Capsule Composition of Pneumococcal Serotype 19A Subtypes	0:71	Polysaccharide Capsule Composition of Pneumococcal Serotype 19A Subtypes Is Unaltered among Subtypes and Independent of the Nutritional Environment.
27550933	1	40	dep	19A	158:160	arg1	Serotype					149:156	Serotype	149:156	Serotype	149:156	Serotype 19A strains have emerged as a cause of invasive pneumococcal disease after the introduction of the 7-valent pneumococcal conjugate vaccine (PCV7), and serotype 19A has now been included in the recent 13-valent vaccine (PCV13).
27550933	0	41	theme	Pneumococcal	38:49	arg1	Serotype					51:58	Pneumococcal Serotype	38:58	Pneumococcal Serotype 19A Subtypes	38:71	Polysaccharide Capsule Composition of Pneumococcal Serotype 19A Subtypes Is Unaltered among Subtypes and Independent of the Nutritional Environment.
27550933	5	42	theme	significant	1064:1074	arg1	increase					1085:1092	a significant relative increase	1062:1092	a significant relative increase of subtype 19A-II	1062:1110	After the introduction of PCV7, a significant relative increase of subtype 19A-II and decrease of 19A-I occurred.
27550933	4	43	theme	high-performance	886:901	arg1	HPLC					926:929	HPLC	926:929	HPLC	926:929	We therefore analyzed the distribution of 19A subtypes collected within a Swiss national surveillance program and determined capsule composition under different nutritional conditions with high-performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS), and nuclear magnetic resonance (NMR) spectroscopy.
27550933	4	43	theme	high-performance	886:901	arg1	chromatography					910:923	high-performance liquid chromatography	886:923	high-performance liquid chromatography (HPLC)	886:930	We therefore analyzed the distribution of 19A subtypes collected within a Swiss national surveillance program and determined capsule composition under different nutritional conditions with high-performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS), and nuclear magnetic resonance (NMR) spectroscopy.
27550933	0	44	theme	19A	60:62	arg1	Subtypes					64:71	Pneumococcal Serotype 19A Subtypes	38:71	Pneumococcal Serotype 19A Subtypes	38:71	Polysaccharide Capsule Composition of Pneumococcal Serotype 19A Subtypes Is Unaltered among Subtypes and Independent of the Nutritional Environment.
27550933	9	45	theme	other	1894:1898	arg1	factors					1910:1916	other virulence factors	1894:1916	other virulence factors	1894:1916	However, capsule composition analysis of emerging 19A clones is recommended in cases where there is no other explanation for a selective advantage, such as antibiotic resistance or loss or acquisition of other virulence factors.
27550933	4	46	theme	different	848:856	arg1	conditions					870:879	different nutritional conditions	848:879	different nutritional conditions with high-performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS), and nuclear magnetic resonance (NMR) spectroscopy	848:1027	We therefore analyzed the distribution of 19A subtypes collected within a Swiss national surveillance program and determined capsule composition under different nutritional conditions with high-performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS), and nuclear magnetic resonance (NMR) spectroscopy.
27550933	9	47	theme	factors	1910:1916	arg1	acquisition					1879:1889	acquisition	1879:1889	acquisition	1879:1889	However, capsule composition analysis of emerging 19A clones is recommended in cases where there is no other explanation for a selective advantage, such as antibiotic resistance or loss or acquisition of other virulence factors.
27550933	9	47	theme	factors	1910:1916	arg1	loss					1871:1874	loss	1871:1874	loss	1871:1874	However, capsule composition analysis of emerging 19A clones is recommended in cases where there is no other explanation for a selective advantage, such as antibiotic resistance or loss or acquisition of other virulence factors.
27550933	9	47	theme	factors	1910:1916	arg1	resistance					1857:1866	antibiotic resistance	1846:1866	antibiotic resistance	1846:1866	However, capsule composition analysis of emerging 19A clones is recommended in cases where there is no other explanation for a selective advantage, such as antibiotic resistance or loss or acquisition of other virulence factors.
27550933	1	48	theme	7-valent	257:264	arg1	PCV7					298:301	PCV7	298:301	PCV7	298:301	Serotype 19A strains have emerged as a cause of invasive pneumococcal disease after the introduction of the 7-valent pneumococcal conjugate vaccine (PCV7), and serotype 19A has now been included in the recent 13-valent vaccine (PCV13).
27550933	1	48	theme	7-valent	257:264	arg1	vaccine					289:295	the 7-valent pneumococcal conjugate vaccine	253:295	the 7-valent pneumococcal conjugate vaccine (PCV7)	253:302	Serotype 19A strains have emerged as a cause of invasive pneumococcal disease after the introduction of the 7-valent pneumococcal conjugate vaccine (PCV7), and serotype 19A has now been included in the recent 13-valent vaccine (PCV13).
27550933	1	49	theme	conjugate	279:287	arg1	PCV7					298:301	PCV7	298:301	PCV7	298:301	Serotype 19A strains have emerged as a cause of invasive pneumococcal disease after the introduction of the 7-valent pneumococcal conjugate vaccine (PCV7), and serotype 19A has now been included in the recent 13-valent vaccine (PCV13).
27550933	1	49	theme	conjugate	279:287	arg1	vaccine					289:295	the 7-valent pneumococcal conjugate vaccine	253:295	the 7-valent pneumococcal conjugate vaccine (PCV7)	253:302	Serotype 19A strains have emerged as a cause of invasive pneumococcal disease after the introduction of the 7-valent pneumococcal conjugate vaccine (PCV7), and serotype 19A has now been included in the recent 13-valent vaccine (PCV13).
27550933	7	50	theme	environment	1504:1514	arg1	dependent					1475:1483	dependent	1475:1483	dependent	1475:1483	In summary, our study suggests that no structural variance dependent of the nutritional environment or the subtype exists.
27550933	4	51	theme	gas	933:935	arg1	GC-MS					971:975	GC-MS	971:975	GC-MS	971:975	We therefore analyzed the distribution of 19A subtypes collected within a Swiss national surveillance program and determined capsule composition under different nutritional conditions with high-performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS), and nuclear magnetic resonance (NMR) spectroscopy.
27550933	4	51	theme	gas	933:935	arg1	spectrometry					957:968	gas chromatography-mass spectrometry	933:968	gas chromatography-mass spectrometry (GC-MS)	933:976	We therefore analyzed the distribution of 19A subtypes collected within a Swiss national surveillance program and determined capsule composition under different nutritional conditions with high-performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS), and nuclear magnetic resonance (NMR) spectroscopy.
27550933	8	52	theme	structure	1671:1679	arg1	variant					1681:1687	a capsule structure variant	1661:1687	a capsule structure variant	1661:1687	The serotype 19A subtype shift observed after the introduction of the PCV7 can therefore not be explained by selection of a capsule structure variant.
27550933	2	53	theme	capsular	440:447	arg1	serotype					449:456	capsular serotype	440:456	capsular serotype	440:456	Genetic analysis has revealed at least three different capsular serotype 19A subtypes, and nutritional environment-dependent variation of the 19A capsule structure has been reported.
27550933	4	54	theme	liquid	903:908	arg1	HPLC					926:929	HPLC	926:929	HPLC	926:929	We therefore analyzed the distribution of 19A subtypes collected within a Swiss national surveillance program and determined capsule composition under different nutritional conditions with high-performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS), and nuclear magnetic resonance (NMR) spectroscopy.
27550933	4	54	theme	liquid	903:908	arg1	chromatography					910:923	high-performance liquid chromatography	886:923	high-performance liquid chromatography (HPLC)	886:930	We therefore analyzed the distribution of 19A subtypes collected within a Swiss national surveillance program and determined capsule composition under different nutritional conditions with high-performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS), and nuclear magnetic resonance (NMR) spectroscopy.
27550933	3	55	theme	Pneumococcal	568:579	arg1	effectiveness					589:601	Pneumococcal vaccine effectiveness	568:601	Pneumococcal vaccine effectiveness	568:601	Pneumococcal vaccine effectiveness and serotyping accuracy might be impaired by structural differences in serotype 19A capsules.
27550933	0	56	theme	Environment	136:146	arg1	Independent					105:115	Independent	105:115	Independent	105:115	Polysaccharide Capsule Composition of Pneumococcal Serotype 19A Subtypes Is Unaltered among Subtypes and Independent of the Nutritional Environment.
27550933	1	57	theme	invasive	197:204	arg1	disease					219:225	invasive pneumococcal disease	197:225	invasive pneumococcal disease	197:225	Serotype 19A strains have emerged as a cause of invasive pneumococcal disease after the introduction of the 7-valent pneumococcal conjugate vaccine (PCV7), and serotype 19A has now been included in the recent 13-valent vaccine (PCV13).
27550933	3	58	theme	serotyping	607:616	arg1	accuracy					618:625	serotyping accuracy	607:625	serotyping accuracy	607:625	Pneumococcal vaccine effectiveness and serotyping accuracy might be impaired by structural differences in serotype 19A capsules.
27550933	1	59	theme	disease	219:225	arg1	cause					188:192	a cause	186:192	a cause of invasive pneumococcal disease	186:225	Serotype 19A strains have emerged as a cause of invasive pneumococcal disease after the introduction of the 7-valent pneumococcal conjugate vaccine (PCV7), and serotype 19A has now been included in the recent 13-valent vaccine (PCV13).
27550933	4	60	theme	nuclear	983:989	arg1	NMR					1011:1013	NMR	1011:1013	NMR	1011:1013	We therefore analyzed the distribution of 19A subtypes collected within a Swiss national surveillance program and determined capsule composition under different nutritional conditions with high-performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS), and nuclear magnetic resonance (NMR) spectroscopy.
27550933	4	60	theme	nuclear	983:989	arg1	resonance					1000:1008	nuclear magnetic resonance	983:1008	nuclear magnetic resonance (NMR) spectroscopy	983:1027	We therefore analyzed the distribution of 19A subtypes collected within a Swiss national surveillance program and determined capsule composition under different nutritional conditions with high-performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS), and nuclear magnetic resonance (NMR) spectroscopy.
27550933	6	61	theme	repeat	1347:1352	arg1	unit					1354:1357	a trisaccharide repeat unit	1331:1357	a trisaccharide repeat unit composed of rhamnose, N-acetyl-mannosamine, and glucose	1331:1413	Chemical analyses showed no difference in the composition as well as the linkage of 19A subtype capsular saccharides grown in defined and undefined growth media, which is consistent with a trisaccharide repeat unit composed of rhamnose, N-acetyl-mannosamine, and glucose.
27550933	5	62	theme	subtype	1097:1103	arg1	19A-II					1105:1110	subtype 19A-II	1097:1110	subtype 19A-II	1097:1110	After the introduction of PCV7, a significant relative increase of subtype 19A-II and decrease of 19A-I occurred.
27550933	6	63	theme	saccharides	1249:1259	arg1	difference					1172:1181	no difference	1169:1181	no difference in the composition as well as the linkage of 19A subtype capsular saccharides grown in defined and undefined growth media, which is consistent with a trisaccharide repeat unit composed of rhamnose, N-acetyl-mannosamine, and glucose	1169:1413	Chemical analyses showed no difference in the composition as well as the linkage of 19A subtype capsular saccharides grown in defined and undefined growth media, which is consistent with a trisaccharide repeat unit composed of rhamnose, N-acetyl-mannosamine, and glucose.
27550933	6	63	theme	saccharides	1249:1259	arg1	linkage					1217:1223	the linkage	1213:1223	no difference in the composition as well as the linkage of 19A subtype capsular saccharides grown in defined and undefined growth media, which is consistent with a trisaccharide repeat unit composed of rhamnose, N-acetyl-mannosamine, and glucose	1169:1413	Chemical analyses showed no difference in the composition as well as the linkage of 19A subtype capsular saccharides grown in defined and undefined growth media, which is consistent with a trisaccharide repeat unit composed of rhamnose, N-acetyl-mannosamine, and glucose.
27550933	4	64	theme	subtypes	743:750	arg1	distribution					723:734	the distribution	719:734	the distribution of 19A subtypes collected within a Swiss national surveillance program	719:805	We therefore analyzed the distribution of 19A subtypes collected within a Swiss national surveillance program and determined capsule composition under different nutritional conditions with high-performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS), and nuclear magnetic resonance (NMR) spectroscopy.
27550933	1	65	theme	19A	158:160	arg1	strains					162:168	Serotype 19A strains	149:168	Serotype 19A strains	149:168	Serotype 19A strains have emerged as a cause of invasive pneumococcal disease after the introduction of the 7-valent pneumococcal conjugate vaccine (PCV7), and serotype 19A has now been included in the recent 13-valent vaccine (PCV13).
27550933	2	66	theme	nutritional	476:486	arg1	variation					510:518	nutritional environment-dependent variation	476:518	nutritional environment-dependent variation of the 19A capsule structure	476:547	Genetic analysis has revealed at least three different capsular serotype 19A subtypes, and nutritional environment-dependent variation of the 19A capsule structure has been reported.
27550933	6	67	theme	subtype	1232:1238	arg1	saccharides					1249:1259	19A subtype capsular saccharides	1228:1259	19A subtype capsular saccharides grown in defined and undefined growth media, which is consistent with a trisaccharide repeat unit composed of rhamnose, N-acetyl-mannosamine, and glucose	1228:1413	Chemical analyses showed no difference in the composition as well as the linkage of 19A subtype capsular saccharides grown in defined and undefined growth media, which is consistent with a trisaccharide repeat unit composed of rhamnose, N-acetyl-mannosamine, and glucose.
27550933	8	68	theme	subtype	1556:1562	arg1	shift					1564:1568	The serotype 19A subtype shift	1539:1568	The serotype 19A subtype shift observed after the introduction of the PCV7	1539:1612	The serotype 19A subtype shift observed after the introduction of the PCV7 can therefore not be explained by selection of a capsule structure variant.
27550933	4	69	theme	resonance	1000:1008	arg1	spectroscopy					1016:1027	nuclear magnetic resonance (NMR) spectroscopy	983:1027	nuclear magnetic resonance (NMR) spectroscopy	983:1027	We therefore analyzed the distribution of 19A subtypes collected within a Swiss national surveillance program and determined capsule composition under different nutritional conditions with high-performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS), and nuclear magnetic resonance (NMR) spectroscopy.
27550933	4	70	theme	surveillance	786:797	arg1	program					799:805	a Swiss national surveillance program	769:805	a Swiss national surveillance program	769:805	We therefore analyzed the distribution of 19A subtypes collected within a Swiss national surveillance program and determined capsule composition under different nutritional conditions with high-performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS), and nuclear magnetic resonance (NMR) spectroscopy.
27550933	6	71	from	linkage	1217:1223	arg1	composition					1190:1200	the composition	1186:1200	the composition	1186:1200	Chemical analyses showed no difference in the composition as well as the linkage of 19A subtype capsular saccharides grown in defined and undefined growth media, which is consistent with a trisaccharide repeat unit composed of rhamnose, N-acetyl-mannosamine, and glucose.
27550933	1	72	theme	recent	351:356	arg1	PCV13					377:381	PCV13	377:381	PCV13	377:381	Serotype 19A strains have emerged as a cause of invasive pneumococcal disease after the introduction of the 7-valent pneumococcal conjugate vaccine (PCV7), and serotype 19A has now been included in the recent 13-valent vaccine (PCV13).
27550933	1	72	theme	recent	351:356	arg1	vaccine					368:374	the recent 13-valent vaccine	347:374	the recent 13-valent vaccine (PCV13)	347:382	Serotype 19A strains have emerged as a cause of invasive pneumococcal disease after the introduction of the 7-valent pneumococcal conjugate vaccine (PCV7), and serotype 19A has now been included in the recent 13-valent vaccine (PCV13).
27550933	0	73	theme	Capsule	15:21	arg1	Composition					23:33	Polysaccharide Capsule Composition	0:33	Polysaccharide Capsule Composition of Pneumococcal Serotype 19A Subtypes	0:71	Polysaccharide Capsule Composition of Pneumococcal Serotype 19A Subtypes Is Unaltered among Subtypes and Independent of the Nutritional Environment.
27550933	2	74	theme	structure	539:547	arg1	variation					510:518	nutritional environment-dependent variation	476:518	nutritional environment-dependent variation of the 19A capsule structure	476:547	Genetic analysis has revealed at least three different capsular serotype 19A subtypes, and nutritional environment-dependent variation of the 19A capsule structure has been reported.
27550933	9	75	theme	antibiotic	1846:1855	arg1	resistance					1857:1866	antibiotic resistance	1846:1866	antibiotic resistance	1846:1866	However, capsule composition analysis of emerging 19A clones is recommended in cases where there is no other explanation for a selective advantage, such as antibiotic resistance or loss or acquisition of other virulence factors.
27550933	8	76	theme	PCV7	1609:1612	arg1	introduction					1589:1600	the introduction	1585:1600	the introduction of the PCV7	1585:1612	The serotype 19A subtype shift observed after the introduction of the PCV7 can therefore not be explained by selection of a capsule structure variant.
27550933	4	77	theme	Swiss	771:775	arg1	program					799:805	a Swiss national surveillance program	769:805	a Swiss national surveillance program	769:805	We therefore analyzed the distribution of 19A subtypes collected within a Swiss national surveillance program and determined capsule composition under different nutritional conditions with high-performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS), and nuclear magnetic resonance (NMR) spectroscopy.
27550933	0	78	theme	Serotype	51:58	arg1	Subtypes					64:71	Pneumococcal Serotype 19A Subtypes	38:71	Pneumococcal Serotype 19A Subtypes	38:71	Polysaccharide Capsule Composition of Pneumococcal Serotype 19A Subtypes Is Unaltered among Subtypes and Independent of the Nutritional Environment.
27550933	2	79	theme	19A	458:460	arg1	subtypes					462:469	at least three different capsular serotype 19A subtypes	415:469	at least three different capsular serotype 19A subtypes	415:469	Genetic analysis has revealed at least three different capsular serotype 19A subtypes, and nutritional environment-dependent variation of the 19A capsule structure has been reported.
27550933	3	80	theme	structural	648:657	arg1	differences					659:669	structural differences	648:669	structural differences in serotype 19A capsules	648:694	Pneumococcal vaccine effectiveness and serotyping accuracy might be impaired by structural differences in serotype 19A capsules.
27550933	4	81	theme	nutritional	858:868	arg1	conditions					870:879	different nutritional conditions	848:879	different nutritional conditions with high-performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS), and nuclear magnetic resonance (NMR) spectroscopy	848:1027	We therefore analyzed the distribution of 19A subtypes collected within a Swiss national surveillance program and determined capsule composition under different nutritional conditions with high-performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS), and nuclear magnetic resonance (NMR) spectroscopy.
27550933	6	82	from	difference	1172:1181	arg1	composition					1190:1200	the composition	1186:1200	the composition	1186:1200	Chemical analyses showed no difference in the composition as well as the linkage of 19A subtype capsular saccharides grown in defined and undefined growth media, which is consistent with a trisaccharide repeat unit composed of rhamnose, N-acetyl-mannosamine, and glucose.
27550933	2	83	theme	Genetic	385:391	arg1	analysis					393:400	Genetic analysis	385:400	Genetic analysis	385:400	Genetic analysis has revealed at least three different capsular serotype 19A subtypes, and nutritional environment-dependent variation of the 19A capsule structure has been reported.
27550933	0	84	theme	Subtypes	64:71	arg1	Composition					23:33	Polysaccharide Capsule Composition	0:33	Polysaccharide Capsule Composition of Pneumococcal Serotype 19A Subtypes	0:71	Polysaccharide Capsule Composition of Pneumococcal Serotype 19A Subtypes Is Unaltered among Subtypes and Independent of the Nutritional Environment.
27550933	5	85	theme	relative	1076:1083	arg1	increase					1085:1092	a significant relative increase	1062:1092	a significant relative increase of subtype 19A-II	1062:1110	After the introduction of PCV7, a significant relative increase of subtype 19A-II and decrease of 19A-I occurred.
27550933	2	86	theme	capsule	531:537	arg1	structure					539:547	the 19A capsule structure	523:547	the 19A capsule structure	523:547	Genetic analysis has revealed at least three different capsular serotype 19A subtypes, and nutritional environment-dependent variation of the 19A capsule structure has been reported.
27550933	6	87	with	consistent	1315:1324	arg1	unit					1354:1357	a trisaccharide repeat unit	1331:1357	a trisaccharide repeat unit composed of rhamnose, N-acetyl-mannosamine, and glucose	1331:1413	Chemical analyses showed no difference in the composition as well as the linkage of 19A subtype capsular saccharides grown in defined and undefined growth media, which is consistent with a trisaccharide repeat unit composed of rhamnose, N-acetyl-mannosamine, and glucose.
27550933	3	88	theme	19A	683:685	arg1	capsules					687:694	serotype 19A capsules	674:694	serotype 19A capsules	674:694	Pneumococcal vaccine effectiveness and serotyping accuracy might be impaired by structural differences in serotype 19A capsules.
27550933	4	89	theme	capsule	822:828	arg1	composition					830:840	capsule composition	822:840	capsule composition	822:840	We therefore analyzed the distribution of 19A subtypes collected within a Swiss national surveillance program and determined capsule composition under different nutritional conditions with high-performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS), and nuclear magnetic resonance (NMR) spectroscopy.
27550933	5	90	theme	PCV7	1056:1059	arg1	introduction					1040:1051	the introduction	1036:1051	the introduction of PCV7	1036:1059	After the introduction of PCV7, a significant relative increase of subtype 19A-II and decrease of 19A-I occurred.
27550933	9	91	theme	virulence	1900:1908	arg1	factors					1910:1916	other virulence factors	1894:1916	other virulence factors	1894:1916	However, capsule composition analysis of emerging 19A clones is recommended in cases where there is no other explanation for a selective advantage, such as antibiotic resistance or loss or acquisition of other virulence factors.
27760230	12	0	theme	mono-	1980:1984	arg1	concentrations					2006:2019	mono- and divalent cation concentrations	1980:2019	mono- and divalent cation concentrations	1980:2019	Electrophoretic mobility of the studied strains was also affected by the structure of the bacterial population (i.e., live/dead cell ratio), medium composition (ionic strength and mono- and divalent cation concentrations), and pH.
27760230	5	1	theme	Physicochemical	435:449	arg1	properties					451:460	Physicochemical properties	435:460	Physicochemical properties of bacterial cells	435:479	Physicochemical properties of bacterial cells are underpinned by the chemical composition of their envelope surrounding the cells, and depend on various environmental conditions.
27760230	12	2	theme	medium	1941:1946	arg1	composition					1948:1958	medium composition	1941:1958	medium composition (ionic strength and mono- and divalent cation concentrations)	1941:2020	Electrophoretic mobility of the studied strains was also affected by the structure of the bacterial population (i.e., live/dead cell ratio), medium composition (ionic strength and mono- and divalent cation concentrations), and pH.
27760230	7	3	theme	cell	1096:1099	arg1	viability					1101:1109	cell viability	1096:1109	cell viability	1096:1109	trifolii strain 24.2 and its derivatives producing various levels of exopolysaccharide (EPS), namely, pssA mutant Rt5819 deficient in EPS synthesis, rosR mutant Rt2472 producing diminished amounts of this polysaccharide, and two EPS-overproducing strains, Rt24.2(pBA1) and Rt24.2(pBR1), under different growth conditions (medium type, bacterial culture age, cell viability, and pH).
27760230	7	3	theme	cell	1096:1099	arg1	conditions					1048:1057	different growth conditions	1031:1057	different growth conditions (medium type, bacterial culture age, cell viability, and pH)	1031:1118	trifolii strain 24.2 and its derivatives producing various levels of exopolysaccharide (EPS), namely, pssA mutant Rt5819 deficient in EPS synthesis, rosR mutant Rt2472 producing diminished amounts of this polysaccharide, and two EPS-overproducing strains, Rt24.2(pBA1) and Rt24.2(pBR1), under different growth conditions (medium type, bacterial culture age, cell viability, and pH).
27760230	8	4	theme	rhizobial	1204:1212	arg1	cells					1214:1218	rhizobial cells	1204:1218	rhizobial cells	1204:1218	We established that EPS plays an essential role in the electrophoretic mobility of rhizobial cells, and that higher amounts of EPS produced resulted in greater negative electrophoretic mobility and higher acidity (lower pKapp,av) of the bacterial cell surface.
27760230	1	5	with	Strain	69:74	arg1	Content					145:151	Different Extracellular Polysaccharide Content	106:151	Different Extracellular Polysaccharide Content	106:151	trifolii Strain 24.2 and Its Derivatives with Different Extracellular Polysaccharide Content.
27760230	12	6	theme	cell	1928:1931	arg1	population					1900:1909	the bacterial population	1886:1909	the bacterial population (i.e., live/dead cell ratio)	1886:1938	Electrophoretic mobility of the studied strains was also affected by the structure of the bacterial population (i.e., live/dead cell ratio), medium composition (ionic strength and mono- and divalent cation concentrations), and pH.
27760230	12	6	theme	cell	1928:1931	arg1	ratio					1933:1937	live/dead cell ratio	1918:1937	live/dead cell ratio	1918:1937	Electrophoretic mobility of the studied strains was also affected by the structure of the bacterial population (i.e., live/dead cell ratio), medium composition (ionic strength and mono- and divalent cation concentrations), and pH.
27760230	11	7	theme	energy	1735:1740	arg1	measurements					1668:1679	measurements	1668:1679	measurements of water contact angle, surface free energy, and free energy of bacterial surface-water-bacterial surface interaction	1668:1797	This was determined by measurements of water contact angle, surface free energy, and free energy of bacterial surface-water-bacterial surface interaction.
27760230	7	8	theme	bacterial	1073:1081	arg1	age					1091:1093	bacterial culture age	1073:1093	bacterial culture age	1073:1093	trifolii strain 24.2 and its derivatives producing various levels of exopolysaccharide (EPS), namely, pssA mutant Rt5819 deficient in EPS synthesis, rosR mutant Rt2472 producing diminished amounts of this polysaccharide, and two EPS-overproducing strains, Rt24.2(pBA1) and Rt24.2(pBR1), under different growth conditions (medium type, bacterial culture age, cell viability, and pH).
27760230	7	8	theme	bacterial	1073:1081	arg1	conditions					1048:1057	different growth conditions	1031:1057	different growth conditions (medium type, bacterial culture age, cell viability, and pH)	1031:1118	trifolii strain 24.2 and its derivatives producing various levels of exopolysaccharide (EPS), namely, pssA mutant Rt5819 deficient in EPS synthesis, rosR mutant Rt2472 producing diminished amounts of this polysaccharide, and two EPS-overproducing strains, Rt24.2(pBA1) and Rt24.2(pBR1), under different growth conditions (medium type, bacterial culture age, cell viability, and pH).
27760230	7	9	theme	pssA	840:843	arg1	Rt5819					852:857	pssA mutant Rt5819	840:857	pssA mutant Rt5819 deficient in EPS synthesis, rosR mutant Rt2472 producing diminished amounts of this polysaccharide, and two EPS-overproducing strains, Rt24.2(pBA1) and Rt24.2(pBR1),	840:1023	trifolii strain 24.2 and its derivatives producing various levels of exopolysaccharide (EPS), namely, pssA mutant Rt5819 deficient in EPS synthesis, rosR mutant Rt2472 producing diminished amounts of this polysaccharide, and two EPS-overproducing strains, Rt24.2(pBA1) and Rt24.2(pBR1), under different growth conditions (medium type, bacterial culture age, cell viability, and pH).
27760230	7	9	theme	pssA	840:843	arg1	exopolysaccharide					807:823	exopolysaccharide	807:823	exopolysaccharide (EPS)	807:829	trifolii strain 24.2 and its derivatives producing various levels of exopolysaccharide (EPS), namely, pssA mutant Rt5819 deficient in EPS synthesis, rosR mutant Rt2472 producing diminished amounts of this polysaccharide, and two EPS-overproducing strains, Rt24.2(pBA1) and Rt24.2(pBR1), under different growth conditions (medium type, bacterial culture age, cell viability, and pH).
27760230	3	10	theme	clover	284:289	arg1	plants					291:296	clover plants	284:296	clover plants	284:296	trifolii is a soil bacterium able to establish symbiosis with agriculturally important legumes, i.e., clover plants (Trifolium spp.)
27760230	7	11	theme	rosR	887:890	arg1	Rt2472					899:904	rosR mutant Rt2472	887:904	rosR mutant Rt2472 producing diminished amounts of this polysaccharide	887:956	trifolii strain 24.2 and its derivatives producing various levels of exopolysaccharide (EPS), namely, pssA mutant Rt5819 deficient in EPS synthesis, rosR mutant Rt2472 producing diminished amounts of this polysaccharide, and two EPS-overproducing strains, Rt24.2(pBA1) and Rt24.2(pBR1), under different growth conditions (medium type, bacterial culture age, cell viability, and pH).
27760230	10	12	theme	surface	1566:1572	arg1	charge					1574:1579	negative surface charge	1557:1579	negative surface charge	1557:1579	Moreover, EPS produced by rhizobial strains resulted not only in an increase of negative surface charge but also in increased hydrophobicity of bacterial cell surface.
27760230	11	13	theme	surface	1779:1785	arg1	interaction					1787:1797	bacterial surface-water-bacterial surface interaction	1745:1797	bacterial surface-water-bacterial surface interaction	1745:1797	This was determined by measurements of water contact angle, surface free energy, and free energy of bacterial surface-water-bacterial surface interaction.
27760230	8	14	theme	EPS	1248:1250	arg1	EPS					1248:1250	EPS	1248:1250	EPS produced	1248:1259	We established that EPS plays an essential role in the electrophoretic mobility of rhizobial cells, and that higher amounts of EPS produced resulted in greater negative electrophoretic mobility and higher acidity (lower pKapp,av) of the bacterial cell surface.
27760230	8	14	theme	EPS	1248:1250	arg1	amounts					1237:1243	higher amounts	1230:1243	higher amounts of EPS produced	1230:1259	We established that EPS plays an essential role in the electrophoretic mobility of rhizobial cells, and that higher amounts of EPS produced resulted in greater negative electrophoretic mobility and higher acidity (lower pKapp,av) of the bacterial cell surface.
27760230	11	15	theme	bacterial	1745:1753	arg1	interaction					1787:1797	bacterial surface-water-bacterial surface interaction	1745:1797	bacterial surface-water-bacterial surface interaction	1745:1797	This was determined by measurements of water contact angle, surface free energy, and free energy of bacterial surface-water-bacterial surface interaction.
27760230	7	16	theme	growth	1041:1046	arg1	age					1091:1093	bacterial culture age	1073:1093	bacterial culture age	1073:1093	trifolii strain 24.2 and its derivatives producing various levels of exopolysaccharide (EPS), namely, pssA mutant Rt5819 deficient in EPS synthesis, rosR mutant Rt2472 producing diminished amounts of this polysaccharide, and two EPS-overproducing strains, Rt24.2(pBA1) and Rt24.2(pBR1), under different growth conditions (medium type, bacterial culture age, cell viability, and pH).
27760230	7	16	theme	growth	1041:1046	arg1	viability					1101:1109	cell viability	1096:1109	cell viability	1096:1109	trifolii strain 24.2 and its derivatives producing various levels of exopolysaccharide (EPS), namely, pssA mutant Rt5819 deficient in EPS synthesis, rosR mutant Rt2472 producing diminished amounts of this polysaccharide, and two EPS-overproducing strains, Rt24.2(pBA1) and Rt24.2(pBR1), under different growth conditions (medium type, bacterial culture age, cell viability, and pH).
27760230	7	16	theme	growth	1041:1046	arg1	pH					1116:1117	pH	1116:1117	pH	1116:1117	trifolii strain 24.2 and its derivatives producing various levels of exopolysaccharide (EPS), namely, pssA mutant Rt5819 deficient in EPS synthesis, rosR mutant Rt2472 producing diminished amounts of this polysaccharide, and two EPS-overproducing strains, Rt24.2(pBA1) and Rt24.2(pBR1), under different growth conditions (medium type, bacterial culture age, cell viability, and pH).
27760230	7	16	theme	growth	1041:1046	arg1	conditions					1048:1057	different growth conditions	1031:1057	different growth conditions (medium type, bacterial culture age, cell viability, and pH)	1031:1118	trifolii strain 24.2 and its derivatives producing various levels of exopolysaccharide (EPS), namely, pssA mutant Rt5819 deficient in EPS synthesis, rosR mutant Rt2472 producing diminished amounts of this polysaccharide, and two EPS-overproducing strains, Rt24.2(pBA1) and Rt24.2(pBR1), under different growth conditions (medium type, bacterial culture age, cell viability, and pH).
27760230	7	16	theme	growth	1041:1046	arg1	type					1067:1070	medium type	1060:1070	medium type	1060:1070	trifolii strain 24.2 and its derivatives producing various levels of exopolysaccharide (EPS), namely, pssA mutant Rt5819 deficient in EPS synthesis, rosR mutant Rt2472 producing diminished amounts of this polysaccharide, and two EPS-overproducing strains, Rt24.2(pBA1) and Rt24.2(pBR1), under different growth conditions (medium type, bacterial culture age, cell viability, and pH).
27760230	1	17	theme	Polysaccharide	130:143	arg1	Content					145:151	Different Extracellular Polysaccharide Content	106:151	Different Extracellular Polysaccharide Content	106:151	trifolii Strain 24.2 and Its Derivatives with Different Extracellular Polysaccharide Content.
27760230	12	18	theme	pH	2027:2028	arg1	structure					1873:1881	the structure	1869:1881	the structure of the bacterial population (i.e., live/dead cell ratio), medium composition (ionic strength and mono- and divalent cation concentrations), and pH	1869:2028	Electrophoretic mobility of the studied strains was also affected by the structure of the bacterial population (i.e., live/dead cell ratio), medium composition (ionic strength and mono- and divalent cation concentrations), and pH.
27760230	6	19	theme	surface	683:689	arg1	properties					691:700	cell surface properties	678:700	cell surface properties of a wild-type R. leguminosarum bv	678:735	In this study, we performed a comprehensive characterization of cell surface properties of a wild-type R. leguminosarum bv.
27760230	11	20	theme	angle	1698:1702	arg1	measurements					1668:1679	measurements	1668:1679	measurements of water contact angle, surface free energy, and free energy of bacterial surface-water-bacterial surface interaction	1668:1797	This was determined by measurements of water contact angle, surface free energy, and free energy of bacterial surface-water-bacterial surface interaction.
27760230	9	21	theme	EPS-deficient	1450:1462	arg1	mutant					1469:1474	EPS-deficient pssA mutant	1450:1474	EPS-deficient pssA mutant	1450:1474	From the tested strains, the electrophoretic mobility was lowest in EPS-deficient pssA mutant.
27760230	12	22	theme	divalent	1990:1997	arg1	concentrations					2006:2019	mono- and divalent cation concentrations	1980:2019	mono- and divalent cation concentrations	1980:2019	Electrophoretic mobility of the studied strains was also affected by the structure of the bacterial population (i.e., live/dead cell ratio), medium composition (ionic strength and mono- and divalent cation concentrations), and pH.
27760230	11	23	theme	water	1684:1688	arg1	angle					1698:1702	water contact angle	1684:1702	water contact angle	1684:1702	This was determined by measurements of water contact angle, surface free energy, and free energy of bacterial surface-water-bacterial surface interaction.
27760230	8	24	theme	electrophoretic	1290:1304	arg1	mobility					1306:1313	negative electrophoretic mobility	1281:1313	negative electrophoretic mobility	1281:1313	We established that EPS plays an essential role in the electrophoretic mobility of rhizobial cells, and that higher amounts of EPS produced resulted in greater negative electrophoretic mobility and higher acidity (lower pKapp,av) of the bacterial cell surface.
27760230	6	25	theme	comprehensive	644:656	arg1	characterization					658:673	a comprehensive characterization	642:673	a comprehensive characterization of cell surface properties of a wild-type R. leguminosarum bv	642:735	In this study, we performed a comprehensive characterization of cell surface properties of a wild-type R. leguminosarum bv.
27760230	11	26	theme	energy	1718:1723	arg1	measurements					1668:1679	measurements	1668:1679	measurements of water contact angle, surface free energy, and free energy of bacterial surface-water-bacterial surface interaction	1668:1797	This was determined by measurements of water contact angle, surface free energy, and free energy of bacterial surface-water-bacterial surface interaction.
27760230	3	27	theme	soil	196:199	arg1	trifolii					182:189	trifolii	182:189	trifolii	182:189	trifolii is a soil bacterium able to establish symbiosis with agriculturally important legumes, i.e., clover plants (Trifolium spp.)
27760230	3	27	theme	soil	196:199	arg1	bacterium					201:209	a soil bacterium	194:209	a soil bacterium	194:209	trifolii is a soil bacterium able to establish symbiosis with agriculturally important legumes, i.e., clover plants (Trifolium spp.)
27760230	11	28	theme	surface	1705:1711	arg1	energy					1718:1723	surface free energy	1705:1723	surface free energy	1705:1723	This was determined by measurements of water contact angle, surface free energy, and free energy of bacterial surface-water-bacterial surface interaction.
27760230	7	29	theme	EPS-overproducing	967:983	arg1	strains					985:991	two EPS-overproducing strains	963:991	two EPS-overproducing strains	963:991	trifolii strain 24.2 and its derivatives producing various levels of exopolysaccharide (EPS), namely, pssA mutant Rt5819 deficient in EPS synthesis, rosR mutant Rt2472 producing diminished amounts of this polysaccharide, and two EPS-overproducing strains, Rt24.2(pBA1) and Rt24.2(pBR1), under different growth conditions (medium type, bacterial culture age, cell viability, and pH).
27760230	7	29	theme	EPS-overproducing	967:983	arg1	Rt24.2					1011:1016	Rt24.2	1011:1016	Rt24.2	1011:1016	trifolii strain 24.2 and its derivatives producing various levels of exopolysaccharide (EPS), namely, pssA mutant Rt5819 deficient in EPS synthesis, rosR mutant Rt2472 producing diminished amounts of this polysaccharide, and two EPS-overproducing strains, Rt24.2(pBA1) and Rt24.2(pBR1), under different growth conditions (medium type, bacterial culture age, cell viability, and pH).
27760230	7	29	theme	EPS-overproducing	967:983	arg1	Rt24.2					994:999	Rt24.2	994:999	Rt24.2	994:999	trifolii strain 24.2 and its derivatives producing various levels of exopolysaccharide (EPS), namely, pssA mutant Rt5819 deficient in EPS synthesis, rosR mutant Rt2472 producing diminished amounts of this polysaccharide, and two EPS-overproducing strains, Rt24.2(pBA1) and Rt24.2(pBR1), under different growth conditions (medium type, bacterial culture age, cell viability, and pH).
27760230	7	30	from	Rt2472	899:904	arg1	deficient					859:867	deficient	859:867	deficient	859:867	trifolii strain 24.2 and its derivatives producing various levels of exopolysaccharide (EPS), namely, pssA mutant Rt5819 deficient in EPS synthesis, rosR mutant Rt2472 producing diminished amounts of this polysaccharide, and two EPS-overproducing strains, Rt24.2(pBA1) and Rt24.2(pBR1), under different growth conditions (medium type, bacterial culture age, cell viability, and pH).
27760230	10	31	theme	increased	1593:1601	arg1	hydrophobicity					1603:1616	increased hydrophobicity	1593:1616	increased hydrophobicity of bacterial cell surface	1593:1642	Moreover, EPS produced by rhizobial strains resulted not only in an increase of negative surface charge but also in increased hydrophobicity of bacterial cell surface.
27760230	5	32	theme	environmental	588:600	arg1	conditions					602:611	various environmental conditions	580:611	various environmental conditions	580:611	Physicochemical properties of bacterial cells are underpinned by the chemical composition of their envelope surrounding the cells, and depend on various environmental conditions.
27760230	4	33	theme	Cell	316:319	arg1	properties					329:338	Cell surface properties	316:338	Cell surface properties of rhizobia	316:350	Cell surface properties of rhizobia play an essential role in their interaction with both biotic and abiotic surfaces.
27760230	8	34	theme	lower	1335:1339	arg1	av					1347:1348	av	1347:1348	av	1347:1348	We established that EPS plays an essential role in the electrophoretic mobility of rhizobial cells, and that higher amounts of EPS produced resulted in greater negative electrophoretic mobility and higher acidity (lower pKapp,av) of the bacterial cell surface.
27760230	8	34	theme	lower	1335:1339	arg1	pKapp					1341:1345	lower pKapp	1335:1345	lower pKapp	1335:1345	We established that EPS plays an essential role in the electrophoretic mobility of rhizobial cells, and that higher amounts of EPS produced resulted in greater negative electrophoretic mobility and higher acidity (lower pKapp,av) of the bacterial cell surface.
27760230	6	35	theme	leguminosarum	720:732	arg1	properties					691:700	cell surface properties	678:700	cell surface properties of a wild-type R. leguminosarum bv	678:735	In this study, we performed a comprehensive characterization of cell surface properties of a wild-type R. leguminosarum bv.
27760230	0	36	theme	Surface	0:6	arg1	Properties					8:17	Surface Properties	0:17	Surface Properties of Wild-Type Rhizobium leguminosarum bv	0:57	Surface Properties of Wild-Type Rhizobium leguminosarum bv.
27760230	7	37	theme	polysaccharide	943:956	arg1	amounts					927:933	diminished amounts	916:933	diminished amounts of this polysaccharide	916:956	trifolii strain 24.2 and its derivatives producing various levels of exopolysaccharide (EPS), namely, pssA mutant Rt5819 deficient in EPS synthesis, rosR mutant Rt2472 producing diminished amounts of this polysaccharide, and two EPS-overproducing strains, Rt24.2(pBA1) and Rt24.2(pBR1), under different growth conditions (medium type, bacterial culture age, cell viability, and pH).
27760230	7	37	theme	polysaccharide	943:956	arg1	polysaccharide					943:956	this polysaccharide	938:956	this polysaccharide	938:956	trifolii strain 24.2 and its derivatives producing various levels of exopolysaccharide (EPS), namely, pssA mutant Rt5819 deficient in EPS synthesis, rosR mutant Rt2472 producing diminished amounts of this polysaccharide, and two EPS-overproducing strains, Rt24.2(pBA1) and Rt24.2(pBR1), under different growth conditions (medium type, bacterial culture age, cell viability, and pH).
27760230	3	38	theme	able	211:214	arg1	trifolii					182:189	trifolii	182:189	trifolii	182:189	trifolii is a soil bacterium able to establish symbiosis with agriculturally important legumes, i.e., clover plants (Trifolium spp.)
27760230	3	38	theme	able	211:214	arg1	bacterium					201:209	a soil bacterium	194:209	a soil bacterium	194:209	trifolii is a soil bacterium able to establish symbiosis with agriculturally important legumes, i.e., clover plants (Trifolium spp.)
27760230	6	39	theme	wild-type	707:715	arg1	leguminosarum					720:732	a wild-type R. leguminosarum bv	705:735	a wild-type R. leguminosarum bv	705:735	In this study, we performed a comprehensive characterization of cell surface properties of a wild-type R. leguminosarum bv.
27760230	10	40	theme	cell	1631:1634	arg1	surface					1636:1642	bacterial cell surface	1621:1642	bacterial cell surface	1621:1642	Moreover, EPS produced by rhizobial strains resulted not only in an increase of negative surface charge but also in increased hydrophobicity of bacterial cell surface.
27760230	1	41	theme	Different	106:114	arg1	Content					145:151	Different Extracellular Polysaccharide Content	106:151	Different Extracellular Polysaccharide Content	106:151	trifolii Strain 24.2 and Its Derivatives with Different Extracellular Polysaccharide Content.
27760230	0	42	theme	Rhizobium	32:40	arg1	leguminosarum					42:54	Wild-Type Rhizobium leguminosarum bv	22:57	Wild-Type Rhizobium leguminosarum bv	22:57	Surface Properties of Wild-Type Rhizobium leguminosarum bv.
27760230	12	43	theme	strains	1840:1846	arg1	mobility					1816:1823	Electrophoretic mobility	1800:1823	Electrophoretic mobility of the studied strains	1800:1846	Electrophoretic mobility of the studied strains was also affected by the structure of the bacterial population (i.e., live/dead cell ratio), medium composition (ionic strength and mono- and divalent cation concentrations), and pH.
27760230	10	44	theme	rhizobial	1503:1511	arg1	strains					1513:1519	rhizobial strains	1503:1519	rhizobial strains	1503:1519	Moreover, EPS produced by rhizobial strains resulted not only in an increase of negative surface charge but also in increased hydrophobicity of bacterial cell surface.
27760230	8	45	theme	cell	1368:1371	arg1	surface					1373:1379	the bacterial cell surface	1354:1379	the bacterial cell surface	1354:1379	We established that EPS plays an essential role in the electrophoretic mobility of rhizobial cells, and that higher amounts of EPS produced resulted in greater negative electrophoretic mobility and higher acidity (lower pKapp,av) of the bacterial cell surface.
27760230	5	46	theme	bacterial	465:473	arg1	cells					475:479	bacterial cells	465:479	bacterial cells	465:479	Physicochemical properties of bacterial cells are underpinned by the chemical composition of their envelope surrounding the cells, and depend on various environmental conditions.
27760230	7	47	theme	various	789:795	arg1	levels					797:802	various levels	789:802	various levels of exopolysaccharide (EPS), namely, pssA mutant Rt5819 deficient in EPS synthesis, rosR mutant Rt2472 producing diminished amounts of this polysaccharide, and two EPS-overproducing strains, Rt24.2(pBA1) and Rt24.2(pBR1),	789:1023	trifolii strain 24.2 and its derivatives producing various levels of exopolysaccharide (EPS), namely, pssA mutant Rt5819 deficient in EPS synthesis, rosR mutant Rt2472 producing diminished amounts of this polysaccharide, and two EPS-overproducing strains, Rt24.2(pBA1) and Rt24.2(pBR1), under different growth conditions (medium type, bacterial culture age, cell viability, and pH).
27760230	7	48	dep	conditions	1048:1057	arg1	age					1091:1093	bacterial culture age	1073:1093	bacterial culture age	1073:1093	trifolii strain 24.2 and its derivatives producing various levels of exopolysaccharide (EPS), namely, pssA mutant Rt5819 deficient in EPS synthesis, rosR mutant Rt2472 producing diminished amounts of this polysaccharide, and two EPS-overproducing strains, Rt24.2(pBA1) and Rt24.2(pBR1), under different growth conditions (medium type, bacterial culture age, cell viability, and pH).
27760230	7	48	dep	conditions	1048:1057	arg1	viability					1101:1109	cell viability	1096:1109	cell viability	1096:1109	trifolii strain 24.2 and its derivatives producing various levels of exopolysaccharide (EPS), namely, pssA mutant Rt5819 deficient in EPS synthesis, rosR mutant Rt2472 producing diminished amounts of this polysaccharide, and two EPS-overproducing strains, Rt24.2(pBA1) and Rt24.2(pBR1), under different growth conditions (medium type, bacterial culture age, cell viability, and pH).
27760230	7	48	dep	conditions	1048:1057	arg1	pH					1116:1117	pH	1116:1117	pH	1116:1117	trifolii strain 24.2 and its derivatives producing various levels of exopolysaccharide (EPS), namely, pssA mutant Rt5819 deficient in EPS synthesis, rosR mutant Rt2472 producing diminished amounts of this polysaccharide, and two EPS-overproducing strains, Rt24.2(pBA1) and Rt24.2(pBR1), under different growth conditions (medium type, bacterial culture age, cell viability, and pH).
27760230	7	48	dep	conditions	1048:1057	arg1	conditions					1048:1057	different growth conditions	1031:1057	different growth conditions (medium type, bacterial culture age, cell viability, and pH)	1031:1118	trifolii strain 24.2 and its derivatives producing various levels of exopolysaccharide (EPS), namely, pssA mutant Rt5819 deficient in EPS synthesis, rosR mutant Rt2472 producing diminished amounts of this polysaccharide, and two EPS-overproducing strains, Rt24.2(pBA1) and Rt24.2(pBR1), under different growth conditions (medium type, bacterial culture age, cell viability, and pH).
27760230	7	48	dep	conditions	1048:1057	arg1	type					1067:1070	medium type	1060:1070	medium type	1060:1070	trifolii strain 24.2 and its derivatives producing various levels of exopolysaccharide (EPS), namely, pssA mutant Rt5819 deficient in EPS synthesis, rosR mutant Rt2472 producing diminished amounts of this polysaccharide, and two EPS-overproducing strains, Rt24.2(pBA1) and Rt24.2(pBR1), under different growth conditions (medium type, bacterial culture age, cell viability, and pH).
27760230	7	49	from	synthesis	876:884	arg1	deficient					859:867	deficient	859:867	deficient	859:867	trifolii strain 24.2 and its derivatives producing various levels of exopolysaccharide (EPS), namely, pssA mutant Rt5819 deficient in EPS synthesis, rosR mutant Rt2472 producing diminished amounts of this polysaccharide, and two EPS-overproducing strains, Rt24.2(pBA1) and Rt24.2(pBR1), under different growth conditions (medium type, bacterial culture age, cell viability, and pH).
27760230	12	50	theme	ionic	1961:1965	arg1	strength					1967:1974	ionic strength	1961:1974	ionic strength	1961:1974	Electrophoretic mobility of the studied strains was also affected by the structure of the bacterial population (i.e., live/dead cell ratio), medium composition (ionic strength and mono- and divalent cation concentrations), and pH.
27760230	4	51	theme	rhizobia	343:350	arg1	properties					329:338	Cell surface properties	316:338	Cell surface properties of rhizobia	316:350	Cell surface properties of rhizobia play an essential role in their interaction with both biotic and abiotic surfaces.
27760230	3	52	theme	important	259:267	arg1	legumes					269:275	agriculturally important legumes	244:275	agriculturally important legumes	244:275	trifolii is a soil bacterium able to establish symbiosis with agriculturally important legumes, i.e., clover plants (Trifolium spp.)
27760230	4	53	theme	biotic	406:411	arg1	surfaces					425:432	both biotic and abiotic surfaces	401:432	both biotic and abiotic surfaces	401:432	Cell surface properties of rhizobia play an essential role in their interaction with both biotic and abiotic surfaces.
27760230	7	54	theme	mutant	892:897	arg1	Rt2472					899:904	rosR mutant Rt2472	887:904	rosR mutant Rt2472 producing diminished amounts of this polysaccharide	887:956	trifolii strain 24.2 and its derivatives producing various levels of exopolysaccharide (EPS), namely, pssA mutant Rt5819 deficient in EPS synthesis, rosR mutant Rt2472 producing diminished amounts of this polysaccharide, and two EPS-overproducing strains, Rt24.2(pBA1) and Rt24.2(pBR1), under different growth conditions (medium type, bacterial culture age, cell viability, and pH).
27760230	4	55	theme	abiotic	417:423	arg1	surfaces					425:432	both biotic and abiotic surfaces	401:432	both biotic and abiotic surfaces	401:432	Cell surface properties of rhizobia play an essential role in their interaction with both biotic and abiotic surfaces.
27760230	5	56	theme	envelope	534:541	arg1	composition					513:523	the chemical composition	500:523	the chemical composition of their envelope surrounding the cells	500:563	Physicochemical properties of bacterial cells are underpinned by the chemical composition of their envelope surrounding the cells, and depend on various environmental conditions.
27760230	7	57	theme	culture	1083:1089	arg1	age					1091:1093	bacterial culture age	1073:1093	bacterial culture age	1073:1093	trifolii strain 24.2 and its derivatives producing various levels of exopolysaccharide (EPS), namely, pssA mutant Rt5819 deficient in EPS synthesis, rosR mutant Rt2472 producing diminished amounts of this polysaccharide, and two EPS-overproducing strains, Rt24.2(pBA1) and Rt24.2(pBR1), under different growth conditions (medium type, bacterial culture age, cell viability, and pH).
27760230	7	57	theme	culture	1083:1089	arg1	conditions					1048:1057	different growth conditions	1031:1057	different growth conditions (medium type, bacterial culture age, cell viability, and pH)	1031:1118	trifolii strain 24.2 and its derivatives producing various levels of exopolysaccharide (EPS), namely, pssA mutant Rt5819 deficient in EPS synthesis, rosR mutant Rt2472 producing diminished amounts of this polysaccharide, and two EPS-overproducing strains, Rt24.2(pBA1) and Rt24.2(pBR1), under different growth conditions (medium type, bacterial culture age, cell viability, and pH).
27760230	12	58	theme	composition	1948:1958	arg1	structure					1873:1881	the structure	1869:1881	the structure of the bacterial population (i.e., live/dead cell ratio), medium composition (ionic strength and mono- and divalent cation concentrations), and pH	1869:2028	Electrophoretic mobility of the studied strains was also affected by the structure of the bacterial population (i.e., live/dead cell ratio), medium composition (ionic strength and mono- and divalent cation concentrations), and pH.
27760230	2	59	theme	Rhizobium	154:162	arg1	leguminosarum					164:176	Rhizobium leguminosarum bv	154:179	Rhizobium leguminosarum bv.	154:180	Rhizobium leguminosarum bv.
27760230	7	60	theme	EPS	872:874	arg1	synthesis					876:884	EPS synthesis	872:884	EPS synthesis	872:884	trifolii strain 24.2 and its derivatives producing various levels of exopolysaccharide (EPS), namely, pssA mutant Rt5819 deficient in EPS synthesis, rosR mutant Rt2472 producing diminished amounts of this polysaccharide, and two EPS-overproducing strains, Rt24.2(pBA1) and Rt24.2(pBR1), under different growth conditions (medium type, bacterial culture age, cell viability, and pH).
27760230	9	61	theme	tested	1391:1396	arg1	strains					1398:1404	the tested strains	1387:1404	the tested strains	1387:1404	From the tested strains, the electrophoretic mobility was lowest in EPS-deficient pssA mutant.
27760230	12	62	theme	live/dead	1918:1926	arg1	population					1900:1909	the bacterial population	1886:1909	the bacterial population (i.e., live/dead cell ratio)	1886:1938	Electrophoretic mobility of the studied strains was also affected by the structure of the bacterial population (i.e., live/dead cell ratio), medium composition (ionic strength and mono- and divalent cation concentrations), and pH.
27760230	12	62	theme	live/dead	1918:1926	arg1	ratio					1933:1937	live/dead cell ratio	1918:1937	live/dead cell ratio	1918:1937	Electrophoretic mobility of the studied strains was also affected by the structure of the bacterial population (i.e., live/dead cell ratio), medium composition (ionic strength and mono- and divalent cation concentrations), and pH.
27760230	8	63	theme	cells	1214:1218	arg1	mobility					1192:1199	the electrophoretic mobility	1172:1199	the electrophoretic mobility of rhizobial cells	1172:1218	We established that EPS plays an essential role in the electrophoretic mobility of rhizobial cells, and that higher amounts of EPS produced resulted in greater negative electrophoretic mobility and higher acidity (lower pKapp,av) of the bacterial cell surface.
27760230	7	64	theme	medium	1060:1065	arg1	conditions					1048:1057	different growth conditions	1031:1057	different growth conditions (medium type, bacterial culture age, cell viability, and pH)	1031:1118	trifolii strain 24.2 and its derivatives producing various levels of exopolysaccharide (EPS), namely, pssA mutant Rt5819 deficient in EPS synthesis, rosR mutant Rt2472 producing diminished amounts of this polysaccharide, and two EPS-overproducing strains, Rt24.2(pBA1) and Rt24.2(pBR1), under different growth conditions (medium type, bacterial culture age, cell viability, and pH).
27760230	7	64	theme	medium	1060:1065	arg1	type					1067:1070	medium type	1060:1070	medium type	1060:1070	trifolii strain 24.2 and its derivatives producing various levels of exopolysaccharide (EPS), namely, pssA mutant Rt5819 deficient in EPS synthesis, rosR mutant Rt2472 producing diminished amounts of this polysaccharide, and two EPS-overproducing strains, Rt24.2(pBA1) and Rt24.2(pBR1), under different growth conditions (medium type, bacterial culture age, cell viability, and pH).
27760230	7	65	theme	deficient	859:867	arg1	Rt5819					852:857	pssA mutant Rt5819	840:857	pssA mutant Rt5819 deficient in EPS synthesis, rosR mutant Rt2472 producing diminished amounts of this polysaccharide, and two EPS-overproducing strains, Rt24.2(pBA1) and Rt24.2(pBR1),	840:1023	trifolii strain 24.2 and its derivatives producing various levels of exopolysaccharide (EPS), namely, pssA mutant Rt5819 deficient in EPS synthesis, rosR mutant Rt2472 producing diminished amounts of this polysaccharide, and two EPS-overproducing strains, Rt24.2(pBA1) and Rt24.2(pBR1), under different growth conditions (medium type, bacterial culture age, cell viability, and pH).
27760230	7	65	theme	deficient	859:867	arg1	exopolysaccharide					807:823	exopolysaccharide	807:823	exopolysaccharide (EPS)	807:829	trifolii strain 24.2 and its derivatives producing various levels of exopolysaccharide (EPS), namely, pssA mutant Rt5819 deficient in EPS synthesis, rosR mutant Rt2472 producing diminished amounts of this polysaccharide, and two EPS-overproducing strains, Rt24.2(pBA1) and Rt24.2(pBR1), under different growth conditions (medium type, bacterial culture age, cell viability, and pH).
27760230	8	66	dep	acidity	1326:1332	arg1	av					1347:1348	av	1347:1348	av	1347:1348	We established that EPS plays an essential role in the electrophoretic mobility of rhizobial cells, and that higher amounts of EPS produced resulted in greater negative electrophoretic mobility and higher acidity (lower pKapp,av) of the bacterial cell surface.
27760230	8	66	dep	acidity	1326:1332	arg1	pKapp					1341:1345	lower pKapp	1335:1345	lower pKapp	1335:1345	We established that EPS plays an essential role in the electrophoretic mobility of rhizobial cells, and that higher amounts of EPS produced resulted in greater negative electrophoretic mobility and higher acidity (lower pKapp,av) of the bacterial cell surface.
27760230	1	67	with	Derivatives	89:99	arg1	Content					145:151	Different Extracellular Polysaccharide Content	106:151	Different Extracellular Polysaccharide Content	106:151	trifolii Strain 24.2 and Its Derivatives with Different Extracellular Polysaccharide Content.
27760230	3	68	theme	Trifolium	299:307	arg1	spp					309:311	Trifolium spp	299:311	Trifolium spp.	299:312	trifolii is a soil bacterium able to establish symbiosis with agriculturally important legumes, i.e., clover plants (Trifolium spp.)
27760230	12	69	theme	cation	1999:2004	arg1	concentrations					2006:2019	mono- and divalent cation concentrations	1980:2019	mono- and divalent cation concentrations	1980:2019	Electrophoretic mobility of the studied strains was also affected by the structure of the bacterial population (i.e., live/dead cell ratio), medium composition (ionic strength and mono- and divalent cation concentrations), and pH.
27760230	10	70	theme	charge	1574:1579	arg1	increase					1545:1552	an increase	1542:1552	an increase of negative surface charge	1542:1579	Moreover, EPS produced by rhizobial strains resulted not only in an increase of negative surface charge but also in increased hydrophobicity of bacterial cell surface.
27760230	11	71	theme	free	1730:1733	arg1	energy					1735:1740	free energy	1730:1740	free energy of bacterial surface-water-bacterial surface interaction	1730:1797	This was determined by measurements of water contact angle, surface free energy, and free energy of bacterial surface-water-bacterial surface interaction.
27760230	7	72	theme	trifolii	738:745	arg1	strain					747:752	trifolii strain 24.2	738:757	trifolii strain 24.2	738:757	trifolii strain 24.2 and its derivatives producing various levels of exopolysaccharide (EPS), namely, pssA mutant Rt5819 deficient in EPS synthesis, rosR mutant Rt2472 producing diminished amounts of this polysaccharide, and two EPS-overproducing strains, Rt24.2(pBA1) and Rt24.2(pBR1), under different growth conditions (medium type, bacterial culture age, cell viability, and pH).
27760230	7	73	theme	mutant	845:850	arg1	Rt5819					852:857	pssA mutant Rt5819	840:857	pssA mutant Rt5819 deficient in EPS synthesis, rosR mutant Rt2472 producing diminished amounts of this polysaccharide, and two EPS-overproducing strains, Rt24.2(pBA1) and Rt24.2(pBR1),	840:1023	trifolii strain 24.2 and its derivatives producing various levels of exopolysaccharide (EPS), namely, pssA mutant Rt5819 deficient in EPS synthesis, rosR mutant Rt2472 producing diminished amounts of this polysaccharide, and two EPS-overproducing strains, Rt24.2(pBA1) and Rt24.2(pBR1), under different growth conditions (medium type, bacterial culture age, cell viability, and pH).
27760230	7	73	theme	mutant	845:850	arg1	exopolysaccharide					807:823	exopolysaccharide	807:823	exopolysaccharide (EPS)	807:829	trifolii strain 24.2 and its derivatives producing various levels of exopolysaccharide (EPS), namely, pssA mutant Rt5819 deficient in EPS synthesis, rosR mutant Rt2472 producing diminished amounts of this polysaccharide, and two EPS-overproducing strains, Rt24.2(pBA1) and Rt24.2(pBR1), under different growth conditions (medium type, bacterial culture age, cell viability, and pH).
27760230	9	74	theme	electrophoretic	1411:1425	arg1	lowest					1440:1445	lowest	1440:1445	lowest	1440:1445	From the tested strains, the electrophoretic mobility was lowest in EPS-deficient pssA mutant.
27760230	9	74	theme	electrophoretic	1411:1425	arg1	mobility					1427:1434	the electrophoretic mobility	1407:1434	the electrophoretic mobility	1407:1434	From the tested strains, the electrophoretic mobility was lowest in EPS-deficient pssA mutant.
27760230	12	75	theme	population	1900:1909	arg1	structure					1873:1881	the structure	1869:1881	the structure of the bacterial population (i.e., live/dead cell ratio), medium composition (ionic strength and mono- and divalent cation concentrations), and pH	1869:2028	Electrophoretic mobility of the studied strains was also affected by the structure of the bacterial population (i.e., live/dead cell ratio), medium composition (ionic strength and mono- and divalent cation concentrations), and pH.
27760230	8	76	theme	higher	1230:1235	arg1	EPS					1248:1250	EPS	1248:1250	EPS produced	1248:1259	We established that EPS plays an essential role in the electrophoretic mobility of rhizobial cells, and that higher amounts of EPS produced resulted in greater negative electrophoretic mobility and higher acidity (lower pKapp,av) of the bacterial cell surface.
27760230	8	76	theme	higher	1230:1235	arg1	amounts					1237:1243	higher amounts	1230:1243	higher amounts of EPS produced	1230:1259	We established that EPS plays an essential role in the electrophoretic mobility of rhizobial cells, and that higher amounts of EPS produced resulted in greater negative electrophoretic mobility and higher acidity (lower pKapp,av) of the bacterial cell surface.
27760230	10	77	theme	negative	1557:1564	arg1	charge					1574:1579	negative surface charge	1557:1579	negative surface charge	1557:1579	Moreover, EPS produced by rhizobial strains resulted not only in an increase of negative surface charge but also in increased hydrophobicity of bacterial cell surface.
27760230	11	78	theme	interaction	1787:1797	arg1	angle					1698:1702	water contact angle	1684:1702	water contact angle	1684:1702	This was determined by measurements of water contact angle, surface free energy, and free energy of bacterial surface-water-bacterial surface interaction.
27760230	11	78	theme	interaction	1787:1797	arg1	energy					1735:1740	free energy	1730:1740	free energy of bacterial surface-water-bacterial surface interaction	1730:1797	This was determined by measurements of water contact angle, surface free energy, and free energy of bacterial surface-water-bacterial surface interaction.
27760230	11	78	theme	interaction	1787:1797	arg1	energy					1718:1723	surface free energy	1705:1723	surface free energy	1705:1723	This was determined by measurements of water contact angle, surface free energy, and free energy of bacterial surface-water-bacterial surface interaction.
27760230	7	79	theme	different	1031:1039	arg1	age					1091:1093	bacterial culture age	1073:1093	bacterial culture age	1073:1093	trifolii strain 24.2 and its derivatives producing various levels of exopolysaccharide (EPS), namely, pssA mutant Rt5819 deficient in EPS synthesis, rosR mutant Rt2472 producing diminished amounts of this polysaccharide, and two EPS-overproducing strains, Rt24.2(pBA1) and Rt24.2(pBR1), under different growth conditions (medium type, bacterial culture age, cell viability, and pH).
27760230	7	79	theme	different	1031:1039	arg1	viability					1101:1109	cell viability	1096:1109	cell viability	1096:1109	trifolii strain 24.2 and its derivatives producing various levels of exopolysaccharide (EPS), namely, pssA mutant Rt5819 deficient in EPS synthesis, rosR mutant Rt2472 producing diminished amounts of this polysaccharide, and two EPS-overproducing strains, Rt24.2(pBA1) and Rt24.2(pBR1), under different growth conditions (medium type, bacterial culture age, cell viability, and pH).
27760230	7	79	theme	different	1031:1039	arg1	pH					1116:1117	pH	1116:1117	pH	1116:1117	trifolii strain 24.2 and its derivatives producing various levels of exopolysaccharide (EPS), namely, pssA mutant Rt5819 deficient in EPS synthesis, rosR mutant Rt2472 producing diminished amounts of this polysaccharide, and two EPS-overproducing strains, Rt24.2(pBA1) and Rt24.2(pBR1), under different growth conditions (medium type, bacterial culture age, cell viability, and pH).
27760230	7	79	theme	different	1031:1039	arg1	conditions					1048:1057	different growth conditions	1031:1057	different growth conditions (medium type, bacterial culture age, cell viability, and pH)	1031:1118	trifolii strain 24.2 and its derivatives producing various levels of exopolysaccharide (EPS), namely, pssA mutant Rt5819 deficient in EPS synthesis, rosR mutant Rt2472 producing diminished amounts of this polysaccharide, and two EPS-overproducing strains, Rt24.2(pBA1) and Rt24.2(pBR1), under different growth conditions (medium type, bacterial culture age, cell viability, and pH).
27760230	7	79	theme	different	1031:1039	arg1	type					1067:1070	medium type	1060:1070	medium type	1060:1070	trifolii strain 24.2 and its derivatives producing various levels of exopolysaccharide (EPS), namely, pssA mutant Rt5819 deficient in EPS synthesis, rosR mutant Rt2472 producing diminished amounts of this polysaccharide, and two EPS-overproducing strains, Rt24.2(pBA1) and Rt24.2(pBR1), under different growth conditions (medium type, bacterial culture age, cell viability, and pH).
27760230	1	80	theme	Extracellular	116:128	arg1	Content					145:151	Different Extracellular Polysaccharide Content	106:151	Different Extracellular Polysaccharide Content	106:151	trifolii Strain 24.2 and Its Derivatives with Different Extracellular Polysaccharide Content.
27760230	8	81	theme	electrophoretic	1176:1190	arg1	mobility					1192:1199	the electrophoretic mobility	1172:1199	the electrophoretic mobility of rhizobial cells	1172:1218	We established that EPS plays an essential role in the electrophoretic mobility of rhizobial cells, and that higher amounts of EPS produced resulted in greater negative electrophoretic mobility and higher acidity (lower pKapp,av) of the bacterial cell surface.
27760230	6	82	theme	properties	691:700	arg1	characterization					658:673	a comprehensive characterization	642:673	a comprehensive characterization of cell surface properties of a wild-type R. leguminosarum bv	642:735	In this study, we performed a comprehensive characterization of cell surface properties of a wild-type R. leguminosarum bv.
27760230	11	83	theme	surface-water-bacterial	1755:1777	arg1	interaction					1787:1797	bacterial surface-water-bacterial surface interaction	1745:1797	bacterial surface-water-bacterial surface interaction	1745:1797	This was determined by measurements of water contact angle, surface free energy, and free energy of bacterial surface-water-bacterial surface interaction.
27760230	5	84	theme	various	580:586	arg1	conditions					602:611	various environmental conditions	580:611	various environmental conditions	580:611	Physicochemical properties of bacterial cells are underpinned by the chemical composition of their envelope surrounding the cells, and depend on various environmental conditions.
27760230	6	85	theme	cell	678:681	arg1	properties					691:700	cell surface properties	678:700	cell surface properties of a wild-type R. leguminosarum bv	678:735	In this study, we performed a comprehensive characterization of cell surface properties of a wild-type R. leguminosarum bv.
27760230	9	86	theme	pssA	1464:1467	arg1	mutant					1469:1474	EPS-deficient pssA mutant	1450:1474	EPS-deficient pssA mutant	1450:1474	From the tested strains, the electrophoretic mobility was lowest in EPS-deficient pssA mutant.
27760230	8	87	theme	essential	1154:1162	arg1	role					1164:1167	an essential role	1151:1167	an essential role	1151:1167	We established that EPS plays an essential role in the electrophoretic mobility of rhizobial cells, and that higher amounts of EPS produced resulted in greater negative electrophoretic mobility and higher acidity (lower pKapp,av) of the bacterial cell surface.
27760230	7	88	from	strains	985:991	arg1	deficient					859:867	deficient	859:867	deficient	859:867	trifolii strain 24.2 and its derivatives producing various levels of exopolysaccharide (EPS), namely, pssA mutant Rt5819 deficient in EPS synthesis, rosR mutant Rt2472 producing diminished amounts of this polysaccharide, and two EPS-overproducing strains, Rt24.2(pBA1) and Rt24.2(pBR1), under different growth conditions (medium type, bacterial culture age, cell viability, and pH).
27760230	3	89	dep	bacterium	201:209	arg1	spp					309:311	Trifolium spp	299:311	Trifolium spp.	299:312	trifolii is a soil bacterium able to establish symbiosis with agriculturally important legumes, i.e., clover plants (Trifolium spp.)
27760230	11	90	theme	contact	1690:1696	arg1	angle					1698:1702	water contact angle	1684:1702	water contact angle	1684:1702	This was determined by measurements of water contact angle, surface free energy, and free energy of bacterial surface-water-bacterial surface interaction.
27760230	8	91	theme	negative	1281:1288	arg1	mobility					1306:1313	negative electrophoretic mobility	1281:1313	negative electrophoretic mobility	1281:1313	We established that EPS plays an essential role in the electrophoretic mobility of rhizobial cells, and that higher amounts of EPS produced resulted in greater negative electrophoretic mobility and higher acidity (lower pKapp,av) of the bacterial cell surface.
27760230	12	92	theme	bacterial	1890:1898	arg1	population					1900:1909	the bacterial population	1886:1909	the bacterial population (i.e., live/dead cell ratio)	1886:1938	Electrophoretic mobility of the studied strains was also affected by the structure of the bacterial population (i.e., live/dead cell ratio), medium composition (ionic strength and mono- and divalent cation concentrations), and pH.
27760230	12	92	theme	bacterial	1890:1898	arg1	ratio					1933:1937	live/dead cell ratio	1918:1937	live/dead cell ratio	1918:1937	Electrophoretic mobility of the studied strains was also affected by the structure of the bacterial population (i.e., live/dead cell ratio), medium composition (ionic strength and mono- and divalent cation concentrations), and pH.
27760230	1	93	theme	trifolii	60:67	arg1	Strain					69:74	trifolii Strain 24.2	60:79	trifolii Strain 24.2	60:79	trifolii Strain 24.2 and Its Derivatives with Different Extracellular Polysaccharide Content.
27760230	11	94	theme	free	1713:1716	arg1	energy					1718:1723	surface free energy	1705:1723	surface free energy	1705:1723	This was determined by measurements of water contact angle, surface free energy, and free energy of bacterial surface-water-bacterial surface interaction.
27760230	5	95	theme	cells	475:479	arg1	properties					451:460	Physicochemical properties	435:460	Physicochemical properties of bacterial cells	435:479	Physicochemical properties of bacterial cells are underpinned by the chemical composition of their envelope surrounding the cells, and depend on various environmental conditions.
27760230	12	96	dep	ratio	1933:1937	arg1	i.e.					1912:1915	i.e.	1912:1915	i.e.	1912:1915	Electrophoretic mobility of the studied strains was also affected by the structure of the bacterial population (i.e., live/dead cell ratio), medium composition (ionic strength and mono- and divalent cation concentrations), and pH.
27760230	8	97	theme	higher	1319:1324	arg1	acidity					1326:1332	higher acidity	1319:1332	higher acidity (lower pKapp,av)	1319:1349	We established that EPS plays an essential role in the electrophoretic mobility of rhizobial cells, and that higher amounts of EPS produced resulted in greater negative electrophoretic mobility and higher acidity (lower pKapp,av) of the bacterial cell surface.
27760230	6	98	theme	R.	717:718	arg1	leguminosarum					720:732	a wild-type R. leguminosarum bv	705:735	a wild-type R. leguminosarum bv	705:735	In this study, we performed a comprehensive characterization of cell surface properties of a wild-type R. leguminosarum bv.
27760230	0	99	theme	Wild-Type	22:30	arg1	leguminosarum					42:54	Wild-Type Rhizobium leguminosarum bv	22:57	Wild-Type Rhizobium leguminosarum bv	22:57	Surface Properties of Wild-Type Rhizobium leguminosarum bv.
27760230	12	100	theme	studied	1832:1838	arg1	strains					1840:1846	the studied strains	1828:1846	the studied strains	1828:1846	Electrophoretic mobility of the studied strains was also affected by the structure of the bacterial population (i.e., live/dead cell ratio), medium composition (ionic strength and mono- and divalent cation concentrations), and pH.
27760230	12	101	dep	composition	1948:1958	arg1	strength					1967:1974	ionic strength	1961:1974	ionic strength	1961:1974	Electrophoretic mobility of the studied strains was also affected by the structure of the bacterial population (i.e., live/dead cell ratio), medium composition (ionic strength and mono- and divalent cation concentrations), and pH.
27760230	12	101	dep	composition	1948:1958	arg1	concentrations					2006:2019	mono- and divalent cation concentrations	1980:2019	mono- and divalent cation concentrations	1980:2019	Electrophoretic mobility of the studied strains was also affected by the structure of the bacterial population (i.e., live/dead cell ratio), medium composition (ionic strength and mono- and divalent cation concentrations), and pH.
27760230	5	102	theme	chemical	504:511	arg1	composition					513:523	the chemical composition	500:523	the chemical composition of their envelope surrounding the cells	500:563	Physicochemical properties of bacterial cells are underpinned by the chemical composition of their envelope surrounding the cells, and depend on various environmental conditions.
27760230	10	103	theme	bacterial	1621:1629	arg1	surface					1636:1642	bacterial cell surface	1621:1642	bacterial cell surface	1621:1642	Moreover, EPS produced by rhizobial strains resulted not only in an increase of negative surface charge but also in increased hydrophobicity of bacterial cell surface.
27760230	0	104	theme	leguminosarum	42:54	arg1	Properties					8:17	Surface Properties	0:17	Surface Properties of Wild-Type Rhizobium leguminosarum bv	0:57	Surface Properties of Wild-Type Rhizobium leguminosarum bv.
27760230	4	105	with	interaction	384:394	arg1	surfaces					425:432	both biotic and abiotic surfaces	401:432	both biotic and abiotic surfaces	401:432	Cell surface properties of rhizobia play an essential role in their interaction with both biotic and abiotic surfaces.
27760230	12	106	theme	Electrophoretic	1800:1814	arg1	mobility					1816:1823	Electrophoretic mobility	1800:1823	Electrophoretic mobility of the studied strains	1800:1846	Electrophoretic mobility of the studied strains was also affected by the structure of the bacterial population (i.e., live/dead cell ratio), medium composition (ionic strength and mono- and divalent cation concentrations), and pH.
27760230	8	107	dep	greater	1273:1279	arg1	mobility					1306:1313	negative electrophoretic mobility	1281:1313	negative electrophoretic mobility	1281:1313	We established that EPS plays an essential role in the electrophoretic mobility of rhizobial cells, and that higher amounts of EPS produced resulted in greater negative electrophoretic mobility and higher acidity (lower pKapp,av) of the bacterial cell surface.
27760230	7	108	from	deficient	859:867	arg1	Rt2472					899:904	rosR mutant Rt2472	887:904	rosR mutant Rt2472 producing diminished amounts of this polysaccharide	887:956	trifolii strain 24.2 and its derivatives producing various levels of exopolysaccharide (EPS), namely, pssA mutant Rt5819 deficient in EPS synthesis, rosR mutant Rt2472 producing diminished amounts of this polysaccharide, and two EPS-overproducing strains, Rt24.2(pBA1) and Rt24.2(pBR1), under different growth conditions (medium type, bacterial culture age, cell viability, and pH).
27760230	7	108	from	deficient	859:867	arg1	strains					985:991	two EPS-overproducing strains	963:991	two EPS-overproducing strains	963:991	trifolii strain 24.2 and its derivatives producing various levels of exopolysaccharide (EPS), namely, pssA mutant Rt5819 deficient in EPS synthesis, rosR mutant Rt2472 producing diminished amounts of this polysaccharide, and two EPS-overproducing strains, Rt24.2(pBA1) and Rt24.2(pBR1), under different growth conditions (medium type, bacterial culture age, cell viability, and pH).
27760230	7	108	from	deficient	859:867	arg1	synthesis					876:884	EPS synthesis	872:884	EPS synthesis	872:884	trifolii strain 24.2 and its derivatives producing various levels of exopolysaccharide (EPS), namely, pssA mutant Rt5819 deficient in EPS synthesis, rosR mutant Rt2472 producing diminished amounts of this polysaccharide, and two EPS-overproducing strains, Rt24.2(pBA1) and Rt24.2(pBR1), under different growth conditions (medium type, bacterial culture age, cell viability, and pH).
27760230	7	108	from	deficient	859:867	arg1	Rt24.2					1011:1016	Rt24.2	1011:1016	Rt24.2	1011:1016	trifolii strain 24.2 and its derivatives producing various levels of exopolysaccharide (EPS), namely, pssA mutant Rt5819 deficient in EPS synthesis, rosR mutant Rt2472 producing diminished amounts of this polysaccharide, and two EPS-overproducing strains, Rt24.2(pBA1) and Rt24.2(pBR1), under different growth conditions (medium type, bacterial culture age, cell viability, and pH).
27760230	7	108	from	deficient	859:867	arg1	Rt24.2					994:999	Rt24.2	994:999	Rt24.2	994:999	trifolii strain 24.2 and its derivatives producing various levels of exopolysaccharide (EPS), namely, pssA mutant Rt5819 deficient in EPS synthesis, rosR mutant Rt2472 producing diminished amounts of this polysaccharide, and two EPS-overproducing strains, Rt24.2(pBA1) and Rt24.2(pBR1), under different growth conditions (medium type, bacterial culture age, cell viability, and pH).
27760230	7	109	theme	exopolysaccharide	807:823	arg1	levels					797:802	various levels	789:802	various levels of exopolysaccharide (EPS), namely, pssA mutant Rt5819 deficient in EPS synthesis, rosR mutant Rt2472 producing diminished amounts of this polysaccharide, and two EPS-overproducing strains, Rt24.2(pBA1) and Rt24.2(pBR1),	789:1023	trifolii strain 24.2 and its derivatives producing various levels of exopolysaccharide (EPS), namely, pssA mutant Rt5819 deficient in EPS synthesis, rosR mutant Rt2472 producing diminished amounts of this polysaccharide, and two EPS-overproducing strains, Rt24.2(pBA1) and Rt24.2(pBR1), under different growth conditions (medium type, bacterial culture age, cell viability, and pH).
27760230	4	110	theme	essential	360:368	arg1	role					370:373	an essential role	357:373	an essential role	357:373	Cell surface properties of rhizobia play an essential role in their interaction with both biotic and abiotic surfaces.
27760230	8	111	theme	bacterial	1358:1366	arg1	surface					1373:1379	the bacterial cell surface	1354:1379	the bacterial cell surface	1354:1379	We established that EPS plays an essential role in the electrophoretic mobility of rhizobial cells, and that higher amounts of EPS produced resulted in greater negative electrophoretic mobility and higher acidity (lower pKapp,av) of the bacterial cell surface.
27760230	10	112	theme	surface	1636:1642	arg1	hydrophobicity					1603:1616	increased hydrophobicity	1593:1616	increased hydrophobicity of bacterial cell surface	1593:1642	Moreover, EPS produced by rhizobial strains resulted not only in an increase of negative surface charge but also in increased hydrophobicity of bacterial cell surface.
27760230	7	113	theme	diminished	916:925	arg1	amounts					927:933	diminished amounts	916:933	diminished amounts of this polysaccharide	916:956	trifolii strain 24.2 and its derivatives producing various levels of exopolysaccharide (EPS), namely, pssA mutant Rt5819 deficient in EPS synthesis, rosR mutant Rt2472 producing diminished amounts of this polysaccharide, and two EPS-overproducing strains, Rt24.2(pBA1) and Rt24.2(pBR1), under different growth conditions (medium type, bacterial culture age, cell viability, and pH).
27760230	7	113	theme	diminished	916:925	arg1	polysaccharide					943:956	this polysaccharide	938:956	this polysaccharide	938:956	trifolii strain 24.2 and its derivatives producing various levels of exopolysaccharide (EPS), namely, pssA mutant Rt5819 deficient in EPS synthesis, rosR mutant Rt2472 producing diminished amounts of this polysaccharide, and two EPS-overproducing strains, Rt24.2(pBA1) and Rt24.2(pBR1), under different growth conditions (medium type, bacterial culture age, cell viability, and pH).
27760230	4	114	theme	surface	321:327	arg1	properties					329:338	Cell surface properties	316:338	Cell surface properties of rhizobia	316:350	Cell surface properties of rhizobia play an essential role in their interaction with both biotic and abiotic surfaces.
27760230	3	115	with	symbiosis	229:237	arg1	legumes					269:275	agriculturally important legumes	244:275	agriculturally important legumes	244:275	trifolii is a soil bacterium able to establish symbiosis with agriculturally important legumes, i.e., clover plants (Trifolium spp.)
27760230	3	116	dep	plants	291:296	arg1	i.e.					278:281	i.e.	278:281	i.e.	278:281	trifolii is a soil bacterium able to establish symbiosis with agriculturally important legumes, i.e., clover plants (Trifolium spp.)
25481282	1	0	theme	SF-08	168:172	arg1	mycelia					174:180	mycelia	174:180	mycelia	174:180	The intracellular polysaccharide (IPS) from Flammulina velutipes SF-08 mycelia was isolated and degraded by enzyme and acid.
25481282	0	1	theme	velutipes	86:94	arg1	polysaccharide					57:70	intracellular polysaccharide	43:70	intracellular polysaccharide of Flammulina velutipes SF-08	43:100	Enzymatic and acidic degradation effect on intracellular polysaccharide of Flammulina velutipes SF-08.
25481282	4	2	theme	main	525:528	arg1	monosaccharide					530:543	the main monosaccharide	521:543	the main monosaccharide	521:543	On the basis of chemical composition and antioxidant ability analyses, rhamnose as the main monosaccharide might contribute to the strongest antioxidant capacity.
25481282	5	3	theme	anti-aging	678:687	arg1	activity					769:776	superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity	706:776	superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity	706:776	The in vivo results showed that IPS significantly enhanced the activities of anti-aging enzymes, such as superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity, and reduced the content of lipid peroxidantion (LPO).
25481282	5	3	theme	anti-aging	678:687	arg1	enzymes					689:695	anti-aging enzymes	678:695	anti-aging enzymes	678:695	The in vivo results showed that IPS significantly enhanced the activities of anti-aging enzymes, such as superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity, and reduced the content of lipid peroxidantion (LPO).
25481282	1	4	theme	mycelia	174:180	arg1	velutipes					158:166	Flammulina velutipes	147:166	Flammulina velutipes SF-08 mycelia	147:180	The intracellular polysaccharide (IPS) from Flammulina velutipes SF-08 mycelia was isolated and degraded by enzyme and acid.
25481282	4	5	dep	composition	463:473	arg1	the					441:443	the	441:443	the	441:443	On the basis of chemical composition and antioxidant ability analyses, rhamnose as the main monosaccharide might contribute to the strongest antioxidant capacity.
25481282	4	5	dep	composition	463:473	arg1	analyses					499:506	analyses	499:506	analyses	499:506	On the basis of chemical composition and antioxidant ability analyses, rhamnose as the main monosaccharide might contribute to the strongest antioxidant capacity.
25481282	4	5	dep	composition	463:473	arg1	basis					445:449	basis	445:449	basis	445:449	On the basis of chemical composition and antioxidant ability analyses, rhamnose as the main monosaccharide might contribute to the strongest antioxidant capacity.
25481282	3	6	theme	antioxidant	364:374	arg1	activities					376:385	antioxidant activities	364:385	antioxidant activities in vitro	364:394	The structural features and antioxidant activities in vitro of the isolated fractions were evaluated.
25481282	5	7	theme	enzymes	689:695	arg1	activities					664:673	the activities	660:673	the activities of anti-aging enzymes, such as superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity,	660:777	The in vivo results showed that IPS significantly enhanced the activities of anti-aging enzymes, such as superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity, and reduced the content of lipid peroxidantion (LPO).
25481282	5	8	theme	peroxidase	749:758	arg1	activity					769:776	superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity	706:776	superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity	706:776	The in vivo results showed that IPS significantly enhanced the activities of anti-aging enzymes, such as superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity, and reduced the content of lipid peroxidantion (LPO).
25481282	6	9	theme	natural	885:891	arg1	IPS					862:864	IPS	862:864	IPS	862:864	These results suggested that IPS should be a potent natural polymer and can be developed to be novel functional food.
25481282	6	9	theme	natural	885:891	arg1	polymer					893:899	a potent natural polymer	876:899	a potent natural polymer	876:899	These results suggested that IPS should be a potent natural polymer and can be developed to be novel functional food.
25481282	4	10	theme	antioxidant	479:489	arg1	ability					491:497	antioxidant ability	479:497	antioxidant ability	479:497	On the basis of chemical composition and antioxidant ability analyses, rhamnose as the main monosaccharide might contribute to the strongest antioxidant capacity.
25481282	1	11	from	velutipes	158:166	arg1	IPS					137:139	IPS	137:139	IPS	137:139	The intracellular polysaccharide (IPS) from Flammulina velutipes SF-08 mycelia was isolated and degraded by enzyme and acid.
25481282	1	11	from	velutipes	158:166	arg1	polysaccharide					121:134	The intracellular polysaccharide	103:134	The intracellular polysaccharide (IPS) from Flammulina velutipes SF-08 mycelia	103:180	The intracellular polysaccharide (IPS) from Flammulina velutipes SF-08 mycelia was isolated and degraded by enzyme and acid.
25481282	2	12	theme	cellulose	276:284	arg1	chromatography					286:299	DEAE-52 cellulose chromatography	268:299	DEAE-52 cellulose chromatography	268:299	IPS and its derivative were purified by DEAE-52 cellulose chromatography, and five fractions were obtained.
25481282	0	13	theme	intracellular	43:55	arg1	polysaccharide					57:70	intracellular polysaccharide	43:70	intracellular polysaccharide of Flammulina velutipes SF-08	43:100	Enzymatic and acidic degradation effect on intracellular polysaccharide of Flammulina velutipes SF-08.
25481282	2	14	theme	DEAE-52	268:274	arg1	chromatography					286:299	DEAE-52 cellulose chromatography	268:299	DEAE-52 cellulose chromatography	268:299	IPS and its derivative were purified by DEAE-52 cellulose chromatography, and five fractions were obtained.
25481282	5	15	theme	lipid	806:810	arg1	peroxidantion					812:824	lipid peroxidantion	806:824	lipid peroxidantion (LPO)	806:830	The in vivo results showed that IPS significantly enhanced the activities of anti-aging enzymes, such as superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity, and reduced the content of lipid peroxidantion (LPO).
25481282	5	15	theme	lipid	806:810	arg1	LPO					827:829	LPO	827:829	LPO	827:829	The in vivo results showed that IPS significantly enhanced the activities of anti-aging enzymes, such as superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity, and reduced the content of lipid peroxidantion (LPO).
25481282	0	16	from	effect	33:38	arg1	polysaccharide					57:70	intracellular polysaccharide	43:70	intracellular polysaccharide of Flammulina velutipes SF-08	43:100	Enzymatic and acidic degradation effect on intracellular polysaccharide of Flammulina velutipes SF-08.
25481282	6	17	theme	potent	878:883	arg1	IPS					862:864	IPS	862:864	IPS	862:864	These results suggested that IPS should be a potent natural polymer and can be developed to be novel functional food.
25481282	6	17	theme	potent	878:883	arg1	polymer					893:899	a potent natural polymer	876:899	a potent natural polymer	876:899	These results suggested that IPS should be a potent natural polymer and can be developed to be novel functional food.
25481282	0	18	theme	Enzymatic	0:8	arg1	effect					33:38	Enzymatic and acidic degradation effect	0:38	Enzymatic and acidic degradation effect on intracellular polysaccharide of Flammulina velutipes SF-08.	0:101	Enzymatic and acidic degradation effect on intracellular polysaccharide of Flammulina velutipes SF-08.
25481282	4	19	theme	strongest	569:577	arg1	capacity					591:598	the strongest antioxidant capacity	565:598	the strongest antioxidant capacity	565:598	On the basis of chemical composition and antioxidant ability analyses, rhamnose as the main monosaccharide might contribute to the strongest antioxidant capacity.
25481282	5	20	theme	in	605:606	arg1	results					613:619	The in vivo results	601:619	The in vivo results	601:619	The in vivo results showed that IPS significantly enhanced the activities of anti-aging enzymes, such as superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity, and reduced the content of lipid peroxidantion (LPO).
25481282	0	21	theme	degradation	21:31	arg1	effect					33:38	Enzymatic and acidic degradation effect	0:38	Enzymatic and acidic degradation effect on intracellular polysaccharide of Flammulina velutipes SF-08.	0:101	Enzymatic and acidic degradation effect on intracellular polysaccharide of Flammulina velutipes SF-08.
25481282	5	22	theme	peroxidantion	812:824	arg1	content					795:801	the content	791:801	the content of lipid peroxidantion (LPO)	791:830	The in vivo results showed that IPS significantly enhanced the activities of anti-aging enzymes, such as superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity, and reduced the content of lipid peroxidantion (LPO).
25481282	1	23	theme	intracellular	107:119	arg1	IPS					137:139	IPS	137:139	IPS	137:139	The intracellular polysaccharide (IPS) from Flammulina velutipes SF-08 mycelia was isolated and degraded by enzyme and acid.
25481282	1	23	theme	intracellular	107:119	arg1	polysaccharide					121:134	The intracellular polysaccharide	103:134	The intracellular polysaccharide (IPS) from Flammulina velutipes SF-08 mycelia	103:180	The intracellular polysaccharide (IPS) from Flammulina velutipes SF-08 mycelia was isolated and degraded by enzyme and acid.
25481282	0	24	theme	acidic	14:19	arg1	effect					33:38	Enzymatic and acidic degradation effect	0:38	Enzymatic and acidic degradation effect on intracellular polysaccharide of Flammulina velutipes SF-08.	0:101	Enzymatic and acidic degradation effect on intracellular polysaccharide of Flammulina velutipes SF-08.
25481282	4	25	theme	chemical	454:461	arg1	composition					463:473	chemical composition	454:473	chemical composition	454:473	On the basis of chemical composition and antioxidant ability analyses, rhamnose as the main monosaccharide might contribute to the strongest antioxidant capacity.
25481282	4	26	theme	antioxidant	579:589	arg1	capacity					591:598	the strongest antioxidant capacity	565:598	the strongest antioxidant capacity	565:598	On the basis of chemical composition and antioxidant ability analyses, rhamnose as the main monosaccharide might contribute to the strongest antioxidant capacity.
25481282	6	27	theme	functional	934:943	arg1	food					945:948	novel functional food	928:948	novel functional food	928:948	These results suggested that IPS should be a potent natural polymer and can be developed to be novel functional food.
25481282	6	28	theme	novel	928:932	arg1	food					945:948	novel functional food	928:948	novel functional food	928:948	These results suggested that IPS should be a potent natural polymer and can be developed to be novel functional food.
25481282	3	29	theme	isolated	403:410	arg1	fractions					412:420	the isolated fractions	399:420	the isolated fractions	399:420	The structural features and antioxidant activities in vitro of the isolated fractions were evaluated.
25481282	5	30	theme	glutathione	737:747	arg1	GSH-Px					761:766	GSH-Px	761:766	GSH-Px	761:766	The in vivo results showed that IPS significantly enhanced the activities of anti-aging enzymes, such as superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity, and reduced the content of lipid peroxidantion (LPO).
25481282	5	30	theme	glutathione	737:747	arg1	peroxidase					749:758	glutathione peroxidase	737:758	glutathione peroxidase (GSH-Px)	737:767	The in vivo results showed that IPS significantly enhanced the activities of anti-aging enzymes, such as superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity, and reduced the content of lipid peroxidantion (LPO).
25481282	3	31	theme	fractions	412:420	arg1	features					351:358	The structural features	336:358	The structural features	336:358	The structural features and antioxidant activities in vitro of the isolated fractions were evaluated.
25481282	3	31	theme	fractions	412:420	arg1	activities					376:385	antioxidant activities	364:385	antioxidant activities in vitro	364:394	The structural features and antioxidant activities in vitro of the isolated fractions were evaluated.
25481282	5	32	theme	superoxide	706:715	arg1	SOD					728:730	SOD	728:730	SOD	728:730	The in vivo results showed that IPS significantly enhanced the activities of anti-aging enzymes, such as superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity, and reduced the content of lipid peroxidantion (LPO).
25481282	5	32	theme	superoxide	706:715	arg1	dismutase					717:725	superoxide dismutase	706:725	superoxide dismutase (SOD)	706:731	The in vivo results showed that IPS significantly enhanced the activities of anti-aging enzymes, such as superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity, and reduced the content of lipid peroxidantion (LPO).
25481282	5	33	theme	dismutase	717:725	arg1	activity					769:776	superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity	706:776	superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity	706:776	The in vivo results showed that IPS significantly enhanced the activities of anti-aging enzymes, such as superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity, and reduced the content of lipid peroxidantion (LPO).
25481282	0	34	theme	Flammulina	75:84	arg1	velutipes					86:94	Flammulina velutipes SF-08	75:100	Flammulina velutipes SF-08	75:100	Enzymatic and acidic degradation effect on intracellular polysaccharide of Flammulina velutipes SF-08.
25481282	1	35	theme	Flammulina	147:156	arg1	velutipes					158:166	Flammulina velutipes	147:166	Flammulina velutipes SF-08 mycelia	147:180	The intracellular polysaccharide (IPS) from Flammulina velutipes SF-08 mycelia was isolated and degraded by enzyme and acid.
25481282	3	36	theme	structural	340:349	arg1	features					351:358	The structural features	336:358	The structural features	336:358	The structural features and antioxidant activities in vitro of the isolated fractions were evaluated.
25481282	5	37	dep	in	605:606	arg1	vivo					608:611	vivo	608:611	vivo	608:611	The in vivo results showed that IPS significantly enhanced the activities of anti-aging enzymes, such as superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity, and reduced the content of lipid peroxidantion (LPO).
27176049	2	0	theme	hydrogel-nanorod	386:401	arg1	composite					403:411	a NIR-responsive hydrogel-nanorod composite	369:411	a NIR-responsive hydrogel-nanorod composite	369:411	MATERIALS & METHODS Gold nanorods were mixed with hyaluronic acid derivatives modified with β-cyclodextrin or adamantane to create a NIR-responsive hydrogel-nanorod composite.
27176049	4	1	dep	DISCUSSION	520:529	arg1	exhibited					571:579	exhibited	571:579	exhibited	571:579	RESULTS & DISCUSSION The hydrogel-nanorod composite material exhibited a rapid response to NIR-irradiation, allowing enhanced release of encapsulated payloads with material heating and network disruption.
27176049	2	2	theme	NIR-responsive	371:384	arg1	composite					403:411	a NIR-responsive hydrogel-nanorod composite	369:411	a NIR-responsive hydrogel-nanorod composite	369:411	MATERIALS & METHODS Gold nanorods were mixed with hyaluronic acid derivatives modified with β-cyclodextrin or adamantane to create a NIR-responsive hydrogel-nanorod composite.
27176049	4	3	theme	payloads	660:667	arg1	release					636:642	enhanced release	627:642	enhanced release of encapsulated payloads with material heating and network disruption	627:712	RESULTS & DISCUSSION The hydrogel-nanorod composite material exhibited a rapid response to NIR-irradiation, allowing enhanced release of encapsulated payloads with material heating and network disruption.
27176049	5	4	theme	light	803:807	arg1	intensity					809:817	the light intensity	799:817	the light intensity	799:817	The release was dependent on the entrapped molecule size, the NIR exposure time and the light intensity.
27176049	4	5	theme	rapid	583:587	arg1	response					589:596	a rapid response	581:596	a rapid response to NIR-irradiation	581:615	RESULTS & DISCUSSION The hydrogel-nanorod composite material exhibited a rapid response to NIR-irradiation, allowing enhanced release of encapsulated payloads with material heating and network disruption.
27176049	5	6	theme	entrapped	748:756	arg1	size					767:770	the entrapped molecule size	744:770	the entrapped molecule size	744:770	The release was dependent on the entrapped molecule size, the NIR exposure time and the light intensity.
27176049	4	7	theme	hydrogel-nanorod	535:550	arg1	material					562:569	The hydrogel-nanorod composite material	531:569	The hydrogel-nanorod composite material	531:569	RESULTS & DISCUSSION The hydrogel-nanorod composite material exhibited a rapid response to NIR-irradiation, allowing enhanced release of encapsulated payloads with material heating and network disruption.
27176049	5	8	theme	molecule	758:765	arg1	size					767:770	the entrapped molecule size	744:770	the entrapped molecule size	744:770	The release was dependent on the entrapped molecule size, the NIR exposure time and the light intensity.
27176049	2	9	theme	acid	299:302	arg1	derivatives					304:314	hyaluronic acid derivatives	288:314	hyaluronic acid derivatives modified with β-cyclodextrin or adamantane to create a NIR-responsive hydrogel-nanorod composite	288:411	MATERIALS & METHODS Gold nanorods were mixed with hyaluronic acid derivatives modified with β-cyclodextrin or adamantane to create a NIR-responsive hydrogel-nanorod composite.
27176049	4	10	theme	material	674:681	arg1	heating					683:689	material heating	674:689	material heating	674:689	RESULTS & DISCUSSION The hydrogel-nanorod composite material exhibited a rapid response to NIR-irradiation, allowing enhanced release of encapsulated payloads with material heating and network disruption.
27176049	2	11	theme	hyaluronic	288:297	arg1	acid					299:302	hyaluronic acid	288:302	hyaluronic acid derivatives modified with β-cyclodextrin or adamantane to create a NIR-responsive hydrogel-nanorod composite	288:411	MATERIALS & METHODS Gold nanorods were mixed with hyaluronic acid derivatives modified with β-cyclodextrin or adamantane to create a NIR-responsive hydrogel-nanorod composite.
27176049	3	12	theme	NIR-triggered	473:485	arg1	release					487:493	NIR-triggered release	473:493	NIR-triggered release	473:493	Microfluidics were used to create responsive microgels and NIR-triggered release was evaluated.
27176049	6	13	theme	light-triggered	976:990	arg1	release					992:998	light-triggered release	976:998	light-triggered release of therapeutics	976:1014	CONCLUSION NIR irradiation of hydrogel-nanorods leads to plasmonic heating and triggered release of encapsulated molecules, a system that has potential for light-triggered release of therapeutics.
27176049	4	14	theme	composite	552:560	arg1	material					562:569	The hydrogel-nanorod composite material	531:569	The hydrogel-nanorod composite material	531:569	RESULTS & DISCUSSION The hydrogel-nanorod composite material exhibited a rapid response to NIR-irradiation, allowing enhanced release of encapsulated payloads with material heating and network disruption.
27176049	6	15	theme	plasmonic	877:885	arg1	heating					887:893	plasmonic heating	877:893	plasmonic heating	877:893	CONCLUSION NIR irradiation of hydrogel-nanorods leads to plasmonic heating and triggered release of encapsulated molecules, a system that has potential for light-triggered release of therapeutics.
27176049	1	16	theme	near	211:214	arg1	light					231:235	near infrared (NIR) light	211:235	near infrared (NIR) light	211:235	AIM To develop a stimulus-responsive material platform capable of releasing entrapped molecules in response to near infrared (NIR) light.
27176049	0	17	theme	Near-infrared	0:12	arg1	light					14:18	Near-infrared light	0:18	Near-infrared light	0:18	Near-infrared light triggered release of molecules from supramolecular hydrogel-nanorod composites.
27176049	4	18	theme	enhanced	627:634	arg1	release					636:642	enhanced release	627:642	enhanced release of encapsulated payloads with material heating and network disruption	627:712	RESULTS & DISCUSSION The hydrogel-nanorod composite material exhibited a rapid response to NIR-irradiation, allowing enhanced release of encapsulated payloads with material heating and network disruption.
27176049	5	19	theme	exposure	781:788	arg1	time					790:793	the NIR exposure time	773:793	the NIR exposure time	773:793	The release was dependent on the entrapped molecule size, the NIR exposure time and the light intensity.
27176049	4	20	theme	encapsulated	647:658	arg1	payloads					660:667	encapsulated payloads	647:667	encapsulated payloads with material heating and network disruption	647:712	RESULTS & DISCUSSION The hydrogel-nanorod composite material exhibited a rapid response to NIR-irradiation, allowing enhanced release of encapsulated payloads with material heating and network disruption.
27176049	1	21	theme	stimulus-responsive	117:135	arg1	platform					146:153	a stimulus-responsive material platform	115:153	a stimulus-responsive material platform capable of releasing entrapped molecules in response to near infrared (NIR) light	115:235	AIM To develop a stimulus-responsive material platform capable of releasing entrapped molecules in response to near infrared (NIR) light.
27176049	0	22	theme	molecules	41:49	arg1	release					30:36	release	30:36	release of molecules from supramolecular hydrogel-nanorod composites	30:97	Near-infrared light triggered release of molecules from supramolecular hydrogel-nanorod composites.
27176049	1	23	dep	near	211:214	arg1	infrared					216:223	infrared	216:223	infrared	216:223	AIM To develop a stimulus-responsive material platform capable of releasing entrapped molecules in response to near infrared (NIR) light.
27176049	2	24	dep	METHODS	250:256	arg1	mixed					277:281	mixed	277:281	were mixed with hyaluronic acid derivatives modified with β-cyclodextrin or adamantane to create a NIR-responsive hydrogel-nanorod composite	272:411	MATERIALS & METHODS Gold nanorods were mixed with hyaluronic acid derivatives modified with β-cyclodextrin or adamantane to create a NIR-responsive hydrogel-nanorod composite.
27176049	3	25	used	used	433:436	arg2	Microfluidics					414:426	Microfluidics	414:426	Microfluidics	414:426	Microfluidics were used to create responsive microgels and NIR-triggered release was evaluated.
27176049	0	26	from	composites	88:97	arg1	release					30:36	release	30:36	release of molecules from supramolecular hydrogel-nanorod composites	30:97	Near-infrared light triggered release of molecules from supramolecular hydrogel-nanorod composites.
27176049	1	27	dep	infrared	216:223	arg1	NIR					226:228	NIR	226:228	NIR	226:228	AIM To develop a stimulus-responsive material platform capable of releasing entrapped molecules in response to near infrared (NIR) light.
27176049	6	28	theme	hydrogel-nanorods	850:866	arg1	irradiation					835:845	CONCLUSION NIR irradiation	820:845	CONCLUSION NIR irradiation of hydrogel-nanorods	820:866	CONCLUSION NIR irradiation of hydrogel-nanorods leads to plasmonic heating and triggered release of encapsulated molecules, a system that has potential for light-triggered release of therapeutics.
27176049	0	29	theme	supramolecular	56:69	arg1	composites					88:97	supramolecular hydrogel-nanorod composites	56:97	supramolecular hydrogel-nanorod composites	56:97	Near-infrared light triggered release of molecules from supramolecular hydrogel-nanorod composites.
27176049	1	30	theme	material	137:144	arg1	platform					146:153	a stimulus-responsive material platform	115:153	a stimulus-responsive material platform capable of releasing entrapped molecules in response to near infrared (NIR) light	115:235	AIM To develop a stimulus-responsive material platform capable of releasing entrapped molecules in response to near infrared (NIR) light.
27176049	3	31	theme	responsive	448:457	arg1	microgels					459:467	responsive microgels	448:467	responsive microgels	448:467	Microfluidics were used to create responsive microgels and NIR-triggered release was evaluated.
27176049	4	32	with	payloads	660:667	arg1	heating					683:689	material heating	674:689	material heating	674:689	RESULTS & DISCUSSION The hydrogel-nanorod composite material exhibited a rapid response to NIR-irradiation, allowing enhanced release of encapsulated payloads with material heating and network disruption.
27176049	4	32	with	payloads	660:667	arg1	disruption					703:712	network disruption	695:712	network disruption	695:712	RESULTS & DISCUSSION The hydrogel-nanorod composite material exhibited a rapid response to NIR-irradiation, allowing enhanced release of encapsulated payloads with material heating and network disruption.
27176049	6	33	theme	NIR	831:833	arg1	irradiation					835:845	CONCLUSION NIR irradiation	820:845	CONCLUSION NIR irradiation of hydrogel-nanorods	820:866	CONCLUSION NIR irradiation of hydrogel-nanorods leads to plasmonic heating and triggered release of encapsulated molecules, a system that has potential for light-triggered release of therapeutics.
27176049	1	34	theme	capable	155:161	arg1	platform					146:153	a stimulus-responsive material platform	115:153	a stimulus-responsive material platform capable of releasing entrapped molecules in response to near infrared (NIR) light	115:235	AIM To develop a stimulus-responsive material platform capable of releasing entrapped molecules in response to near infrared (NIR) light.
27176049	4	35	theme	network	695:701	arg1	disruption					703:712	network disruption	695:712	network disruption	695:712	RESULTS & DISCUSSION The hydrogel-nanorod composite material exhibited a rapid response to NIR-irradiation, allowing enhanced release of encapsulated payloads with material heating and network disruption.
27176049	5	36	theme	NIR	777:779	arg1	time					790:793	the NIR exposure time	773:793	the NIR exposure time	773:793	The release was dependent on the entrapped molecule size, the NIR exposure time and the light intensity.
27176049	6	37	theme	molecules	933:941	arg1	release					909:915	release	909:915	release of encapsulated molecules, a system that has potential for light-triggered release of therapeutics	909:1014	CONCLUSION NIR irradiation of hydrogel-nanorods leads to plasmonic heating and triggered release of encapsulated molecules, a system that has potential for light-triggered release of therapeutics.
27176049	6	38	theme	CONCLUSION	820:829	arg1	irradiation					835:845	CONCLUSION NIR irradiation	820:845	CONCLUSION NIR irradiation of hydrogel-nanorods	820:866	CONCLUSION NIR irradiation of hydrogel-nanorods leads to plasmonic heating and triggered release of encapsulated molecules, a system that has potential for light-triggered release of therapeutics.
27176049	6	39	contain	has	958:960	arg2	potential					962:970	potential	962:970	potential for light-triggered release of therapeutics	962:1014	CONCLUSION NIR irradiation of hydrogel-nanorods leads to plasmonic heating and triggered release of encapsulated molecules, a system that has potential for light-triggered release of therapeutics.
27176049	6	39	contain	has	958:960	arg1	molecules					933:941	encapsulated molecules	920:941	encapsulated molecules	920:941	CONCLUSION NIR irradiation of hydrogel-nanorods leads to plasmonic heating and triggered release of encapsulated molecules, a system that has potential for light-triggered release of therapeutics.
27176049	6	39	contain	has	958:960	arg1	system					946:951	a system	944:951	a system that has potential for light-triggered release of therapeutics	944:1014	CONCLUSION NIR irradiation of hydrogel-nanorods leads to plasmonic heating and triggered release of encapsulated molecules, a system that has potential for light-triggered release of therapeutics.
27176049	6	40	theme	encapsulated	920:931	arg1	molecules					933:941	encapsulated molecules	920:941	encapsulated molecules	920:941	CONCLUSION NIR irradiation of hydrogel-nanorods leads to plasmonic heating and triggered release of encapsulated molecules, a system that has potential for light-triggered release of therapeutics.
27176049	6	40	theme	encapsulated	920:931	arg1	system					946:951	a system	944:951	a system that has potential for light-triggered release of therapeutics	944:1014	CONCLUSION NIR irradiation of hydrogel-nanorods leads to plasmonic heating and triggered release of encapsulated molecules, a system that has potential for light-triggered release of therapeutics.
27176049	6	41	theme	therapeutics	1003:1014	arg1	release					992:998	light-triggered release	976:998	light-triggered release of therapeutics	976:1014	CONCLUSION NIR irradiation of hydrogel-nanorods leads to plasmonic heating and triggered release of encapsulated molecules, a system that has potential for light-triggered release of therapeutics.
27176049	0	42	theme	hydrogel-nanorod	71:86	arg1	composites					88:97	supramolecular hydrogel-nanorod composites	56:97	supramolecular hydrogel-nanorod composites	56:97	Near-infrared light triggered release of molecules from supramolecular hydrogel-nanorod composites.
27176049	2	43	theme	Gold	258:261	arg1	nanorods					263:270	Gold nanorods	258:270	Gold nanorods	258:270	MATERIALS & METHODS Gold nanorods were mixed with hyaluronic acid derivatives modified with β-cyclodextrin or adamantane to create a NIR-responsive hydrogel-nanorod composite.
27176049	1	44	theme	entrapped	176:184	arg1	molecules					186:194	entrapped molecules	176:194	entrapped molecules	176:194	AIM To develop a stimulus-responsive material platform capable of releasing entrapped molecules in response to near infrared (NIR) light.
28465177	0	0	theme	Aureobasdium	87:98	arg1	P16					112:114	Aureobasdium melanogenum P16	87:114	Aureobasdium melanogenum P16	87:114	Simultaneous production of both high molecular weight pullulan and oligosaccharides by Aureobasdium melanogenum P16 isolated from a mangrove ecosystem.
28465177	4	1	theme	yeast	1287:1291	arg1	cultivated					1304:1313	the yeast strain P16 cultivated	1283:1313	the yeast strain P16 cultivated	1283:1313	Most of the oligosaccharides produced by the yeast strain P16 cultivated in the compositionally changed medium had degree of polymerization of 4 and 5.
28465177	2	2	theme	α-amylase	554:562	arg1	activities					540:549	the decreased activities	526:549	the decreased activities of α-amylase, glucoamylase and pullulanase	526:592	The increased Mw of the pullulan was due to the decreased activities of α-amylase, glucoamylase and pullulanase while the decreased pullulan titer was related to the decreased transcriptional levels of the genes encoding 6-P-glucose kinase, glucosyltransferase, α-phosphoglucose mutase, UDPG-pyrophosphorylase and pullulan synthetase.
28465177	1	3	theme	compositional	162:174	arg1	change					176:181	the compositional change	158:181	the compositional change of a pullulan production medium	158:213	After the compositional change of a pullulan production medium, a molecular weight (Mw) of the pullulan produced by Aureobasidium melanogenum P16 was 2.32×106 and a pullulan titer was 44.4g/L while a Mw of the pullulan produced by A. melanogenum P16 grown in the initial medium was only 3.47×105 and a pullulan titer was 65.3g/L.
28465177	3	4	theme	pullulan	1118:1125	arg1	46.4g/L					1159:1165	46.4g/L	1159:1165	46.4g/L	1159:1165	During the 10-L fermentation, when the yeast strain P16 was grown in the initial medium, the pullulan and oligosaccharide titers were 65.5g/L and 7.8g/L, respectively and the Mw of the produced pullulan was 4.42×105 while when the yeast strain P16 was grown in the compositionally changed medium, the pullulan and oligosaccharide titers were 46.4g/L and 27.8g/L, respectively and the Mw of the produced pullulan was 2.6×106.
28465177	3	4	theme	pullulan	1118:1125	arg1	titers					1147:1152	the pullulan and oligosaccharide titers	1114:1152	titers	1147:1152	During the 10-L fermentation, when the yeast strain P16 was grown in the initial medium, the pullulan and oligosaccharide titers were 65.5g/L and 7.8g/L, respectively and the Mw of the produced pullulan was 4.42×105 while when the yeast strain P16 was grown in the compositionally changed medium, the pullulan and oligosaccharide titers were 46.4g/L and 27.8g/L, respectively and the Mw of the produced pullulan was 2.6×106.
28465177	2	5	theme	pullulan	614:621	arg1	related					633:639	related	633:639	related	633:639	The increased Mw of the pullulan was due to the decreased activities of α-amylase, glucoamylase and pullulanase while the decreased pullulan titer was related to the decreased transcriptional levels of the genes encoding 6-P-glucose kinase, glucosyltransferase, α-phosphoglucose mutase, UDPG-pyrophosphorylase and pullulan synthetase.
28465177	2	5	theme	pullulan	614:621	arg1	titer					623:627	the decreased pullulan titer	600:627	the decreased pullulan titer	600:627	The increased Mw of the pullulan was due to the decreased activities of α-amylase, glucoamylase and pullulanase while the decreased pullulan titer was related to the decreased transcriptional levels of the genes encoding 6-P-glucose kinase, glucosyltransferase, α-phosphoglucose mutase, UDPG-pyrophosphorylase and pullulan synthetase.
28465177	5	6	with	pullulan	1425:1432	arg1	levels					1465:1470	high levels	1460:1470	high levels	1460:1470	Therefore, both of the high Mw pullulan and oligosaccharides with high levels were produced by the yeast strain P16.
28465177	3	7	theme	yeast	856:860	arg1	P16					869:871	the yeast strain P16	852:871	the yeast strain P16	852:871	During the 10-L fermentation, when the yeast strain P16 was grown in the initial medium, the pullulan and oligosaccharide titers were 65.5g/L and 7.8g/L, respectively and the Mw of the produced pullulan was 4.42×105 while when the yeast strain P16 was grown in the compositionally changed medium, the pullulan and oligosaccharide titers were 46.4g/L and 27.8g/L, respectively and the Mw of the produced pullulan was 2.6×106.
28465177	3	8	theme	10-L	828:831	arg1	fermentation					833:844	the 10-L fermentation	824:844	the 10-L fermentation	824:844	During the 10-L fermentation, when the yeast strain P16 was grown in the initial medium, the pullulan and oligosaccharide titers were 65.5g/L and 7.8g/L, respectively and the Mw of the produced pullulan was 4.42×105 while when the yeast strain P16 was grown in the compositionally changed medium, the pullulan and oligosaccharide titers were 46.4g/L and 27.8g/L, respectively and the Mw of the produced pullulan was 2.6×106.
28465177	2	9	theme	increased	486:494	arg1	due					519:521	due	519:521	due	519:521	The increased Mw of the pullulan was due to the decreased activities of α-amylase, glucoamylase and pullulanase while the decreased pullulan titer was related to the decreased transcriptional levels of the genes encoding 6-P-glucose kinase, glucosyltransferase, α-phosphoglucose mutase, UDPG-pyrophosphorylase and pullulan synthetase.
28465177	2	9	theme	increased	486:494	arg1	Mw					496:497	The increased Mw	482:497	The increased Mw of the pullulan	482:513	The increased Mw of the pullulan was due to the decreased activities of α-amylase, glucoamylase and pullulanase while the decreased pullulan titer was related to the decreased transcriptional levels of the genes encoding 6-P-glucose kinase, glucosyltransferase, α-phosphoglucose mutase, UDPG-pyrophosphorylase and pullulan synthetase.
28465177	1	10	dep	Aureobasidium	268:280	arg1	melanogenum					282:292	melanogenum	282:292	melanogenum	282:292	After the compositional change of a pullulan production medium, a molecular weight (Mw) of the pullulan produced by Aureobasidium melanogenum P16 was 2.32×106 and a pullulan titer was 44.4g/L while a Mw of the pullulan produced by A. melanogenum P16 grown in the initial medium was only 3.47×105 and a pullulan titer was 65.3g/L.
28465177	2	11	theme	decreased	604:612	arg1	related					633:639	related	633:639	related	633:639	The increased Mw of the pullulan was due to the decreased activities of α-amylase, glucoamylase and pullulanase while the decreased pullulan titer was related to the decreased transcriptional levels of the genes encoding 6-P-glucose kinase, glucosyltransferase, α-phosphoglucose mutase, UDPG-pyrophosphorylase and pullulan synthetase.
28465177	2	11	theme	decreased	604:612	arg1	titer					623:627	the decreased pullulan titer	600:627	the decreased pullulan titer	600:627	The increased Mw of the pullulan was due to the decreased activities of α-amylase, glucoamylase and pullulanase while the decreased pullulan titer was related to the decreased transcriptional levels of the genes encoding 6-P-glucose kinase, glucosyltransferase, α-phosphoglucose mutase, UDPG-pyrophosphorylase and pullulan synthetase.
28465177	3	12	theme	strain	862:867	arg1	P16					869:871	the yeast strain P16	852:871	the yeast strain P16	852:871	During the 10-L fermentation, when the yeast strain P16 was grown in the initial medium, the pullulan and oligosaccharide titers were 65.5g/L and 7.8g/L, respectively and the Mw of the produced pullulan was 4.42×105 while when the yeast strain P16 was grown in the compositionally changed medium, the pullulan and oligosaccharide titers were 46.4g/L and 27.8g/L, respectively and the Mw of the produced pullulan was 2.6×106.
28465177	1	13	dep	A.	383:384	arg1	melanogenum					386:396	melanogenum	386:396	melanogenum	386:396	After the compositional change of a pullulan production medium, a molecular weight (Mw) of the pullulan produced by Aureobasidium melanogenum P16 was 2.32×106 and a pullulan titer was 44.4g/L while a Mw of the pullulan produced by A. melanogenum P16 grown in the initial medium was only 3.47×105 and a pullulan titer was 65.3g/L.
28465177	0	14	theme	melanogenum	100:110	arg1	P16					112:114	Aureobasdium melanogenum P16	87:114	Aureobasdium melanogenum P16	87:114	Simultaneous production of both high molecular weight pullulan and oligosaccharides by Aureobasdium melanogenum P16 isolated from a mangrove ecosystem.
28465177	2	15	theme	decreased	530:538	arg1	activities					540:549	the decreased activities	526:549	the decreased activities of α-amylase, glucoamylase and pullulanase	526:592	The increased Mw of the pullulan was due to the decreased activities of α-amylase, glucoamylase and pullulanase while the decreased pullulan titer was related to the decreased transcriptional levels of the genes encoding 6-P-glucose kinase, glucosyltransferase, α-phosphoglucose mutase, UDPG-pyrophosphorylase and pullulan synthetase.
28465177	1	16	theme	initial	415:421	arg1	medium					423:428	the initial medium	411:428	the initial medium	411:428	After the compositional change of a pullulan production medium, a molecular weight (Mw) of the pullulan produced by Aureobasidium melanogenum P16 was 2.32×106 and a pullulan titer was 44.4g/L while a Mw of the pullulan produced by A. melanogenum P16 grown in the initial medium was only 3.47×105 and a pullulan titer was 65.3g/L.
28465177	5	17	theme	high	1460:1463	arg1	levels					1465:1470	high levels	1460:1470	high levels	1460:1470	Therefore, both of the high Mw pullulan and oligosaccharides with high levels were produced by the yeast strain P16.
28465177	4	18	theme	4	1385:1385	arg1	polymerization					1367:1380	polymerization	1367:1380	polymerization of 4 and 5	1367:1391	Most of the oligosaccharides produced by the yeast strain P16 cultivated in the compositionally changed medium had degree of polymerization of 4 and 5.
28465177	5	19	theme	Mw	1422:1423	arg1	pullulan					1425:1432	the high Mw pullulan	1413:1432	the high Mw pullulan	1413:1432	Therefore, both of the high Mw pullulan and oligosaccharides with high levels were produced by the yeast strain P16.
28465177	2	20	theme	pullulanase	582:592	arg1	activities					540:549	the decreased activities	526:549	the decreased activities of α-amylase, glucoamylase and pullulanase	526:592	The increased Mw of the pullulan was due to the decreased activities of α-amylase, glucoamylase and pullulanase while the decreased pullulan titer was related to the decreased transcriptional levels of the genes encoding 6-P-glucose kinase, glucosyltransferase, α-phosphoglucose mutase, UDPG-pyrophosphorylase and pullulan synthetase.
28465177	3	21	theme	produced	1211:1218	arg1	pullulan					1220:1227	the produced pullulan	1207:1227	the produced pullulan	1207:1227	During the 10-L fermentation, when the yeast strain P16 was grown in the initial medium, the pullulan and oligosaccharide titers were 65.5g/L and 7.8g/L, respectively and the Mw of the produced pullulan was 4.42×105 while when the yeast strain P16 was grown in the compositionally changed medium, the pullulan and oligosaccharide titers were 46.4g/L and 27.8g/L, respectively and the Mw of the produced pullulan was 2.6×106.
28465177	3	22	theme	produced	1002:1009	arg1	pullulan					1011:1018	the produced pullulan	998:1018	the produced pullulan	998:1018	During the 10-L fermentation, when the yeast strain P16 was grown in the initial medium, the pullulan and oligosaccharide titers were 65.5g/L and 7.8g/L, respectively and the Mw of the produced pullulan was 4.42×105 while when the yeast strain P16 was grown in the compositionally changed medium, the pullulan and oligosaccharide titers were 46.4g/L and 27.8g/L, respectively and the Mw of the produced pullulan was 2.6×106.
28465177	2	23	theme	glucoamylase	565:576	arg1	activities					540:549	the decreased activities	526:549	the decreased activities of α-amylase, glucoamylase and pullulanase	526:592	The increased Mw of the pullulan was due to the decreased activities of α-amylase, glucoamylase and pullulanase while the decreased pullulan titer was related to the decreased transcriptional levels of the genes encoding 6-P-glucose kinase, glucosyltransferase, α-phosphoglucose mutase, UDPG-pyrophosphorylase and pullulan synthetase.
28465177	3	24	theme	pullulan	1011:1018	arg1	Mw					992:993	the Mw	988:993	the Mw of the produced pullulan	988:1018	During the 10-L fermentation, when the yeast strain P16 was grown in the initial medium, the pullulan and oligosaccharide titers were 65.5g/L and 7.8g/L, respectively and the Mw of the produced pullulan was 4.42×105 while when the yeast strain P16 was grown in the compositionally changed medium, the pullulan and oligosaccharide titers were 46.4g/L and 27.8g/L, respectively and the Mw of the produced pullulan was 2.6×106.
28465177	3	24	theme	pullulan	1011:1018	arg1	4.42×105					1024:1031	4.42×105	1024:1031	4.42×105	1024:1031	During the 10-L fermentation, when the yeast strain P16 was grown in the initial medium, the pullulan and oligosaccharide titers were 65.5g/L and 7.8g/L, respectively and the Mw of the produced pullulan was 4.42×105 while when the yeast strain P16 was grown in the compositionally changed medium, the pullulan and oligosaccharide titers were 46.4g/L and 27.8g/L, respectively and the Mw of the produced pullulan was 2.6×106.
28465177	2	25	theme	6-P-glucose	703:713	arg1	UDPG-pyrophosphorylase					769:790	UDPG-pyrophosphorylase	769:790	UDPG-pyrophosphorylase	769:790	The increased Mw of the pullulan was due to the decreased activities of α-amylase, glucoamylase and pullulanase while the decreased pullulan titer was related to the decreased transcriptional levels of the genes encoding 6-P-glucose kinase, glucosyltransferase, α-phosphoglucose mutase, UDPG-pyrophosphorylase and pullulan synthetase.
28465177	2	25	theme	6-P-glucose	703:713	arg1	glucosyltransferase					723:741	glucosyltransferase	723:741	glucosyltransferase	723:741	The increased Mw of the pullulan was due to the decreased activities of α-amylase, glucoamylase and pullulanase while the decreased pullulan titer was related to the decreased transcriptional levels of the genes encoding 6-P-glucose kinase, glucosyltransferase, α-phosphoglucose mutase, UDPG-pyrophosphorylase and pullulan synthetase.
28465177	2	25	theme	6-P-glucose	703:713	arg1	mutase					761:766	α-phosphoglucose mutase	744:766	α-phosphoglucose mutase	744:766	The increased Mw of the pullulan was due to the decreased activities of α-amylase, glucoamylase and pullulanase while the decreased pullulan titer was related to the decreased transcriptional levels of the genes encoding 6-P-glucose kinase, glucosyltransferase, α-phosphoglucose mutase, UDPG-pyrophosphorylase and pullulan synthetase.
28465177	2	25	theme	6-P-glucose	703:713	arg1	kinase					715:720	6-P-glucose kinase	703:720	6-P-glucose kinase	703:720	The increased Mw of the pullulan was due to the decreased activities of α-amylase, glucoamylase and pullulanase while the decreased pullulan titer was related to the decreased transcriptional levels of the genes encoding 6-P-glucose kinase, glucosyltransferase, α-phosphoglucose mutase, UDPG-pyrophosphorylase and pullulan synthetase.
28465177	2	25	theme	6-P-glucose	703:713	arg1	synthetase					805:814	pullulan synthetase	796:814	pullulan synthetase	796:814	The increased Mw of the pullulan was due to the decreased activities of α-amylase, glucoamylase and pullulanase while the decreased pullulan titer was related to the decreased transcriptional levels of the genes encoding 6-P-glucose kinase, glucosyltransferase, α-phosphoglucose mutase, UDPG-pyrophosphorylase and pullulan synthetase.
28465177	1	26	theme	pullulan	188:195	arg1	medium					208:213	a pullulan production medium	186:213	a pullulan production medium	186:213	After the compositional change of a pullulan production medium, a molecular weight (Mw) of the pullulan produced by Aureobasidium melanogenum P16 was 2.32×106 and a pullulan titer was 44.4g/L while a Mw of the pullulan produced by A. melanogenum P16 grown in the initial medium was only 3.47×105 and a pullulan titer was 65.3g/L.
28465177	0	27	theme	Simultaneous	0:11	arg1	production					13:22	Simultaneous production	0:22	Simultaneous production of both high molecular weight pullulan and oligosaccharides by Aureobasdium melanogenum P16	0:114	Simultaneous production of both high molecular weight pullulan and oligosaccharides by Aureobasdium melanogenum P16 isolated from a mangrove ecosystem.
28465177	3	28	theme	yeast	1048:1052	arg1	P16					1061:1063	the yeast strain P16	1044:1063	the yeast strain P16	1044:1063	During the 10-L fermentation, when the yeast strain P16 was grown in the initial medium, the pullulan and oligosaccharide titers were 65.5g/L and 7.8g/L, respectively and the Mw of the produced pullulan was 4.42×105 while when the yeast strain P16 was grown in the compositionally changed medium, the pullulan and oligosaccharide titers were 46.4g/L and 27.8g/L, respectively and the Mw of the produced pullulan was 2.6×106.
28465177	3	29	theme	strain	1054:1059	arg1	P16					1061:1063	the yeast strain P16	1044:1063	the yeast strain P16	1044:1063	During the 10-L fermentation, when the yeast strain P16 was grown in the initial medium, the pullulan and oligosaccharide titers were 65.5g/L and 7.8g/L, respectively and the Mw of the produced pullulan was 4.42×105 while when the yeast strain P16 was grown in the compositionally changed medium, the pullulan and oligosaccharide titers were 46.4g/L and 27.8g/L, respectively and the Mw of the produced pullulan was 2.6×106.
28465177	2	30	theme	transcriptional	658:672	arg1	levels					674:679	the decreased transcriptional levels	644:679	the decreased transcriptional levels of the genes encoding 6-P-glucose kinase, glucosyltransferase, α-phosphoglucose mutase, UDPG-pyrophosphorylase and pullulan synthetase	644:814	The increased Mw of the pullulan was due to the decreased activities of α-amylase, glucoamylase and pullulanase while the decreased pullulan titer was related to the decreased transcriptional levels of the genes encoding 6-P-glucose kinase, glucosyltransferase, α-phosphoglucose mutase, UDPG-pyrophosphorylase and pullulan synthetase.
28465177	1	31	theme	production	197:206	arg1	medium					208:213	a pullulan production medium	186:213	a pullulan production medium	186:213	After the compositional change of a pullulan production medium, a molecular weight (Mw) of the pullulan produced by Aureobasidium melanogenum P16 was 2.32×106 and a pullulan titer was 44.4g/L while a Mw of the pullulan produced by A. melanogenum P16 grown in the initial medium was only 3.47×105 and a pullulan titer was 65.3g/L.
28465177	1	32	theme	Aureobasidium	268:280	arg1	P16					294:296	Aureobasidium melanogenum P16	268:296	Aureobasidium melanogenum P16	268:296	After the compositional change of a pullulan production medium, a molecular weight (Mw) of the pullulan produced by Aureobasidium melanogenum P16 was 2.32×106 and a pullulan titer was 44.4g/L while a Mw of the pullulan produced by A. melanogenum P16 grown in the initial medium was only 3.47×105 and a pullulan titer was 65.3g/L.
28465177	0	33	theme	mangrove	132:139	arg1	ecosystem					141:149	a mangrove ecosystem	130:149	a mangrove ecosystem	130:149	Simultaneous production of both high molecular weight pullulan and oligosaccharides by Aureobasdium melanogenum P16 isolated from a mangrove ecosystem.
28465177	3	34	theme	pullulan	910:917	arg1	titers					939:944	the pullulan and oligosaccharide titers	906:944	titers	939:944	During the 10-L fermentation, when the yeast strain P16 was grown in the initial medium, the pullulan and oligosaccharide titers were 65.5g/L and 7.8g/L, respectively and the Mw of the produced pullulan was 4.42×105 while when the yeast strain P16 was grown in the compositionally changed medium, the pullulan and oligosaccharide titers were 46.4g/L and 27.8g/L, respectively and the Mw of the produced pullulan was 2.6×106.
28465177	3	34	theme	pullulan	910:917	arg1	65.5g/L					951:957	65.5g/L	951:957	65.5g/L	951:957	During the 10-L fermentation, when the yeast strain P16 was grown in the initial medium, the pullulan and oligosaccharide titers were 65.5g/L and 7.8g/L, respectively and the Mw of the produced pullulan was 4.42×105 while when the yeast strain P16 was grown in the compositionally changed medium, the pullulan and oligosaccharide titers were 46.4g/L and 27.8g/L, respectively and the Mw of the produced pullulan was 2.6×106.
28465177	1	35	theme	medium	208:213	arg1	change					176:181	the compositional change	158:181	the compositional change of a pullulan production medium	158:213	After the compositional change of a pullulan production medium, a molecular weight (Mw) of the pullulan produced by Aureobasidium melanogenum P16 was 2.32×106 and a pullulan titer was 44.4g/L while a Mw of the pullulan produced by A. melanogenum P16 grown in the initial medium was only 3.47×105 and a pullulan titer was 65.3g/L.
28465177	4	36	contain	had	1353:1355	arg1	Most					1242:1245	Most	1242:1245	Most	1242:1245	Most of the oligosaccharides produced by the yeast strain P16 cultivated in the compositionally changed medium had degree of polymerization of 4 and 5.
28465177	4	36	contain	had	1353:1355	arg1	oligosaccharides					1254:1269	the oligosaccharides	1250:1269	the oligosaccharides produced by the yeast strain P16 cultivated in the compositionally changed medium	1250:1351	Most of the oligosaccharides produced by the yeast strain P16 cultivated in the compositionally changed medium had degree of polymerization of 4 and 5.
28465177	4	36	contain	had	1353:1355	arg2	degree					1357:1362	degree	1357:1362	degree of polymerization of 4 and 5	1357:1391	Most of the oligosaccharides produced by the yeast strain P16 cultivated in the compositionally changed medium had degree of polymerization of 4 and 5.
28465177	2	37	theme	α-phosphoglucose	744:759	arg1	mutase					761:766	α-phosphoglucose mutase	744:766	α-phosphoglucose mutase	744:766	The increased Mw of the pullulan was due to the decreased activities of α-amylase, glucoamylase and pullulanase while the decreased pullulan titer was related to the decreased transcriptional levels of the genes encoding 6-P-glucose kinase, glucosyltransferase, α-phosphoglucose mutase, UDPG-pyrophosphorylase and pullulan synthetase.
28465177	2	37	theme	α-phosphoglucose	744:759	arg1	kinase					715:720	6-P-glucose kinase	703:720	6-P-glucose kinase	703:720	The increased Mw of the pullulan was due to the decreased activities of α-amylase, glucoamylase and pullulanase while the decreased pullulan titer was related to the decreased transcriptional levels of the genes encoding 6-P-glucose kinase, glucosyltransferase, α-phosphoglucose mutase, UDPG-pyrophosphorylase and pullulan synthetase.
28465177	0	38	theme	molecular	37:45	arg1	pullulan					54:61	high molecular weight pullulan	32:61	high molecular weight pullulan	32:61	Simultaneous production of both high molecular weight pullulan and oligosaccharides by Aureobasdium melanogenum P16 isolated from a mangrove ecosystem.
28465177	3	39	theme	initial	890:896	arg1	medium					898:903	the initial medium	886:903	the initial medium	886:903	During the 10-L fermentation, when the yeast strain P16 was grown in the initial medium, the pullulan and oligosaccharide titers were 65.5g/L and 7.8g/L, respectively and the Mw of the produced pullulan was 4.42×105 while when the yeast strain P16 was grown in the compositionally changed medium, the pullulan and oligosaccharide titers were 46.4g/L and 27.8g/L, respectively and the Mw of the produced pullulan was 2.6×106.
28465177	1	40	theme	pullulan	362:369	arg1	3.47×105					439:446	3.47×105	439:446	3.47×105	439:446	After the compositional change of a pullulan production medium, a molecular weight (Mw) of the pullulan produced by Aureobasidium melanogenum P16 was 2.32×106 and a pullulan titer was 44.4g/L while a Mw of the pullulan produced by A. melanogenum P16 grown in the initial medium was only 3.47×105 and a pullulan titer was 65.3g/L.
28465177	1	40	theme	pullulan	362:369	arg1	Mw					352:353	a Mw	350:353	a Mw of the pullulan produced by A. melanogenum P16 grown in the initial medium	350:428	After the compositional change of a pullulan production medium, a molecular weight (Mw) of the pullulan produced by Aureobasidium melanogenum P16 was 2.32×106 and a pullulan titer was 44.4g/L while a Mw of the pullulan produced by A. melanogenum P16 grown in the initial medium was only 3.47×105 and a pullulan titer was 65.3g/L.
28465177	3	41	theme	pullulan	1220:1227	arg1	Mw					1201:1202	the Mw	1197:1202	the Mw of the produced pullulan	1197:1227	During the 10-L fermentation, when the yeast strain P16 was grown in the initial medium, the pullulan and oligosaccharide titers were 65.5g/L and 7.8g/L, respectively and the Mw of the produced pullulan was 4.42×105 while when the yeast strain P16 was grown in the compositionally changed medium, the pullulan and oligosaccharide titers were 46.4g/L and 27.8g/L, respectively and the Mw of the produced pullulan was 2.6×106.
28465177	3	41	theme	pullulan	1220:1227	arg1	2.6×106					1233:1239	2.6×106	1233:1239	2.6×106	1233:1239	During the 10-L fermentation, when the yeast strain P16 was grown in the initial medium, the pullulan and oligosaccharide titers were 65.5g/L and 7.8g/L, respectively and the Mw of the produced pullulan was 4.42×105 while when the yeast strain P16 was grown in the compositionally changed medium, the pullulan and oligosaccharide titers were 46.4g/L and 27.8g/L, respectively and the Mw of the produced pullulan was 2.6×106.
28465177	0	42	theme	high	32:35	arg1	pullulan					54:61	high molecular weight pullulan	32:61	high molecular weight pullulan	32:61	Simultaneous production of both high molecular weight pullulan and oligosaccharides by Aureobasdium melanogenum P16 isolated from a mangrove ecosystem.
28465177	4	43	theme	polymerization	1367:1380	arg1	degree					1357:1362	degree	1357:1362	degree of polymerization of 4 and 5	1357:1391	Most of the oligosaccharides produced by the yeast strain P16 cultivated in the compositionally changed medium had degree of polymerization of 4 and 5.
28465177	4	44	theme	P16	1300:1302	arg1	cultivated					1304:1313	the yeast strain P16 cultivated	1283:1313	the yeast strain P16 cultivated	1283:1313	Most of the oligosaccharides produced by the yeast strain P16 cultivated in the compositionally changed medium had degree of polymerization of 4 and 5.
28465177	1	45	theme	molecular	218:226	arg1	Mw					236:237	Mw	236:237	Mw	236:237	After the compositional change of a pullulan production medium, a molecular weight (Mw) of the pullulan produced by Aureobasidium melanogenum P16 was 2.32×106 and a pullulan titer was 44.4g/L while a Mw of the pullulan produced by A. melanogenum P16 grown in the initial medium was only 3.47×105 and a pullulan titer was 65.3g/L.
28465177	1	45	theme	molecular	218:226	arg1	2.32×106					302:309	2.32×106	302:309	2.32×106	302:309	After the compositional change of a pullulan production medium, a molecular weight (Mw) of the pullulan produced by Aureobasidium melanogenum P16 was 2.32×106 and a pullulan titer was 44.4g/L while a Mw of the pullulan produced by A. melanogenum P16 grown in the initial medium was only 3.47×105 and a pullulan titer was 65.3g/L.
28465177	1	45	theme	molecular	218:226	arg1	weight					228:233	a molecular weight	216:233	a molecular weight (Mw) of the pullulan produced by Aureobasidium melanogenum P16	216:296	After the compositional change of a pullulan production medium, a molecular weight (Mw) of the pullulan produced by Aureobasidium melanogenum P16 was 2.32×106 and a pullulan titer was 44.4g/L while a Mw of the pullulan produced by A. melanogenum P16 grown in the initial medium was only 3.47×105 and a pullulan titer was 65.3g/L.
28465177	0	46	theme	pullulan	54:61	arg1	production					13:22	Simultaneous production	0:22	Simultaneous production of both high molecular weight pullulan and oligosaccharides by Aureobasdium melanogenum P16	0:114	Simultaneous production of both high molecular weight pullulan and oligosaccharides by Aureobasdium melanogenum P16 isolated from a mangrove ecosystem.
28465177	5	47	theme	strain	1499:1504	arg1	P16					1506:1508	the yeast strain P16	1489:1508	the yeast strain P16	1489:1508	Therefore, both of the high Mw pullulan and oligosaccharides with high levels were produced by the yeast strain P16.
28465177	3	48	theme	oligosaccharide	1131:1145	arg1	46.4g/L					1159:1165	46.4g/L	1159:1165	46.4g/L	1159:1165	During the 10-L fermentation, when the yeast strain P16 was grown in the initial medium, the pullulan and oligosaccharide titers were 65.5g/L and 7.8g/L, respectively and the Mw of the produced pullulan was 4.42×105 while when the yeast strain P16 was grown in the compositionally changed medium, the pullulan and oligosaccharide titers were 46.4g/L and 27.8g/L, respectively and the Mw of the produced pullulan was 2.6×106.
28465177	3	48	theme	oligosaccharide	1131:1145	arg1	titers					1147:1152	the pullulan and oligosaccharide titers	1114:1152	titers	1147:1152	During the 10-L fermentation, when the yeast strain P16 was grown in the initial medium, the pullulan and oligosaccharide titers were 65.5g/L and 7.8g/L, respectively and the Mw of the produced pullulan was 4.42×105 while when the yeast strain P16 was grown in the compositionally changed medium, the pullulan and oligosaccharide titers were 46.4g/L and 27.8g/L, respectively and the Mw of the produced pullulan was 2.6×106.
28465177	1	49	theme	pullulan	454:461	arg1	titer					463:467	a pullulan titer	452:467	a pullulan titer	452:467	After the compositional change of a pullulan production medium, a molecular weight (Mw) of the pullulan produced by Aureobasidium melanogenum P16 was 2.32×106 and a pullulan titer was 44.4g/L while a Mw of the pullulan produced by A. melanogenum P16 grown in the initial medium was only 3.47×105 and a pullulan titer was 65.3g/L.
28465177	1	49	theme	pullulan	454:461	arg1	65.3g/L					473:479	65.3g/L	473:479	65.3g/L	473:479	After the compositional change of a pullulan production medium, a molecular weight (Mw) of the pullulan produced by Aureobasidium melanogenum P16 was 2.32×106 and a pullulan titer was 44.4g/L while a Mw of the pullulan produced by A. melanogenum P16 grown in the initial medium was only 3.47×105 and a pullulan titer was 65.3g/L.
28465177	0	50	theme	weight	47:52	arg1	pullulan					54:61	high molecular weight pullulan	32:61	high molecular weight pullulan	32:61	Simultaneous production of both high molecular weight pullulan and oligosaccharides by Aureobasdium melanogenum P16 isolated from a mangrove ecosystem.
28465177	4	51	theme	changed	1338:1344	arg1	medium					1346:1351	the compositionally changed medium	1318:1351	the compositionally changed medium	1318:1351	Most of the oligosaccharides produced by the yeast strain P16 cultivated in the compositionally changed medium had degree of polymerization of 4 and 5.
28465177	2	52	theme	pullulan	506:513	arg1	due					519:521	due	519:521	due	519:521	The increased Mw of the pullulan was due to the decreased activities of α-amylase, glucoamylase and pullulanase while the decreased pullulan titer was related to the decreased transcriptional levels of the genes encoding 6-P-glucose kinase, glucosyltransferase, α-phosphoglucose mutase, UDPG-pyrophosphorylase and pullulan synthetase.
28465177	2	52	theme	pullulan	506:513	arg1	Mw					496:497	The increased Mw	482:497	The increased Mw of the pullulan	482:513	The increased Mw of the pullulan was due to the decreased activities of α-amylase, glucoamylase and pullulanase while the decreased pullulan titer was related to the decreased transcriptional levels of the genes encoding 6-P-glucose kinase, glucosyltransferase, α-phosphoglucose mutase, UDPG-pyrophosphorylase and pullulan synthetase.
28465177	1	53	theme	A.	383:384	arg1	P16					398:400	A. melanogenum P16	383:400	A. melanogenum P16 grown in the initial medium	383:428	After the compositional change of a pullulan production medium, a molecular weight (Mw) of the pullulan produced by Aureobasidium melanogenum P16 was 2.32×106 and a pullulan titer was 44.4g/L while a Mw of the pullulan produced by A. melanogenum P16 grown in the initial medium was only 3.47×105 and a pullulan titer was 65.3g/L.
28465177	2	54	theme	decreased	648:656	arg1	levels					674:679	the decreased transcriptional levels	644:679	the decreased transcriptional levels of the genes encoding 6-P-glucose kinase, glucosyltransferase, α-phosphoglucose mutase, UDPG-pyrophosphorylase and pullulan synthetase	644:814	The increased Mw of the pullulan was due to the decreased activities of α-amylase, glucoamylase and pullulanase while the decreased pullulan titer was related to the decreased transcriptional levels of the genes encoding 6-P-glucose kinase, glucosyltransferase, α-phosphoglucose mutase, UDPG-pyrophosphorylase and pullulan synthetase.
28465177	4	55	theme	strain	1293:1298	arg1	cultivated					1304:1313	the yeast strain P16 cultivated	1283:1313	the yeast strain P16 cultivated	1283:1313	Most of the oligosaccharides produced by the yeast strain P16 cultivated in the compositionally changed medium had degree of polymerization of 4 and 5.
28465177	5	56	theme	yeast	1493:1497	arg1	P16					1506:1508	the yeast strain P16	1489:1508	the yeast strain P16	1489:1508	Therefore, both of the high Mw pullulan and oligosaccharides with high levels were produced by the yeast strain P16.
28465177	3	57	theme	oligosaccharide	923:937	arg1	titers					939:944	the pullulan and oligosaccharide titers	906:944	titers	939:944	During the 10-L fermentation, when the yeast strain P16 was grown in the initial medium, the pullulan and oligosaccharide titers were 65.5g/L and 7.8g/L, respectively and the Mw of the produced pullulan was 4.42×105 while when the yeast strain P16 was grown in the compositionally changed medium, the pullulan and oligosaccharide titers were 46.4g/L and 27.8g/L, respectively and the Mw of the produced pullulan was 2.6×106.
28465177	3	57	theme	oligosaccharide	923:937	arg1	65.5g/L					951:957	65.5g/L	951:957	65.5g/L	951:957	During the 10-L fermentation, when the yeast strain P16 was grown in the initial medium, the pullulan and oligosaccharide titers were 65.5g/L and 7.8g/L, respectively and the Mw of the produced pullulan was 4.42×105 while when the yeast strain P16 was grown in the compositionally changed medium, the pullulan and oligosaccharide titers were 46.4g/L and 27.8g/L, respectively and the Mw of the produced pullulan was 2.6×106.
28465177	2	58	theme	pullulan	796:803	arg1	synthetase					805:814	pullulan synthetase	796:814	pullulan synthetase	796:814	The increased Mw of the pullulan was due to the decreased activities of α-amylase, glucoamylase and pullulanase while the decreased pullulan titer was related to the decreased transcriptional levels of the genes encoding 6-P-glucose kinase, glucosyltransferase, α-phosphoglucose mutase, UDPG-pyrophosphorylase and pullulan synthetase.
28465177	2	58	theme	pullulan	796:803	arg1	kinase					715:720	6-P-glucose kinase	703:720	6-P-glucose kinase	703:720	The increased Mw of the pullulan was due to the decreased activities of α-amylase, glucoamylase and pullulanase while the decreased pullulan titer was related to the decreased transcriptional levels of the genes encoding 6-P-glucose kinase, glucosyltransferase, α-phosphoglucose mutase, UDPG-pyrophosphorylase and pullulan synthetase.
28465177	3	59	theme	changed	1098:1104	arg1	medium					1106:1111	the compositionally changed medium	1078:1111	the compositionally changed medium	1078:1111	During the 10-L fermentation, when the yeast strain P16 was grown in the initial medium, the pullulan and oligosaccharide titers were 65.5g/L and 7.8g/L, respectively and the Mw of the produced pullulan was 4.42×105 while when the yeast strain P16 was grown in the compositionally changed medium, the pullulan and oligosaccharide titers were 46.4g/L and 27.8g/L, respectively and the Mw of the produced pullulan was 2.6×106.
28465177	5	60	with	oligosaccharides	1438:1453	arg1	levels					1465:1470	high levels	1460:1470	high levels	1460:1470	Therefore, both of the high Mw pullulan and oligosaccharides with high levels were produced by the yeast strain P16.
28465177	4	61	theme	5	1391:1391	arg1	polymerization					1367:1380	polymerization	1367:1380	polymerization of 4 and 5	1367:1391	Most of the oligosaccharides produced by the yeast strain P16 cultivated in the compositionally changed medium had degree of polymerization of 4 and 5.
28465177	0	62	theme	oligosaccharides	67:82	arg1	production					13:22	Simultaneous production	0:22	Simultaneous production of both high molecular weight pullulan and oligosaccharides by Aureobasdium melanogenum P16	0:114	Simultaneous production of both high molecular weight pullulan and oligosaccharides by Aureobasdium melanogenum P16 isolated from a mangrove ecosystem.
28465177	2	63	theme	genes	688:692	arg1	levels					674:679	the decreased transcriptional levels	644:679	the decreased transcriptional levels of the genes encoding 6-P-glucose kinase, glucosyltransferase, α-phosphoglucose mutase, UDPG-pyrophosphorylase and pullulan synthetase	644:814	The increased Mw of the pullulan was due to the decreased activities of α-amylase, glucoamylase and pullulanase while the decreased pullulan titer was related to the decreased transcriptional levels of the genes encoding 6-P-glucose kinase, glucosyltransferase, α-phosphoglucose mutase, UDPG-pyrophosphorylase and pullulan synthetase.
28465177	1	64	theme	pullulan	247:254	arg1	Mw					236:237	Mw	236:237	Mw	236:237	After the compositional change of a pullulan production medium, a molecular weight (Mw) of the pullulan produced by Aureobasidium melanogenum P16 was 2.32×106 and a pullulan titer was 44.4g/L while a Mw of the pullulan produced by A. melanogenum P16 grown in the initial medium was only 3.47×105 and a pullulan titer was 65.3g/L.
28465177	1	64	theme	pullulan	247:254	arg1	2.32×106					302:309	2.32×106	302:309	2.32×106	302:309	After the compositional change of a pullulan production medium, a molecular weight (Mw) of the pullulan produced by Aureobasidium melanogenum P16 was 2.32×106 and a pullulan titer was 44.4g/L while a Mw of the pullulan produced by A. melanogenum P16 grown in the initial medium was only 3.47×105 and a pullulan titer was 65.3g/L.
28465177	1	64	theme	pullulan	247:254	arg1	weight					228:233	a molecular weight	216:233	a molecular weight (Mw) of the pullulan produced by Aureobasidium melanogenum P16	216:296	After the compositional change of a pullulan production medium, a molecular weight (Mw) of the pullulan produced by Aureobasidium melanogenum P16 was 2.32×106 and a pullulan titer was 44.4g/L while a Mw of the pullulan produced by A. melanogenum P16 grown in the initial medium was only 3.47×105 and a pullulan titer was 65.3g/L.
28465177	1	65	theme	pullulan	317:324	arg1	44.4g/L					336:342	44.4g/L	336:342	44.4g/L	336:342	After the compositional change of a pullulan production medium, a molecular weight (Mw) of the pullulan produced by Aureobasidium melanogenum P16 was 2.32×106 and a pullulan titer was 44.4g/L while a Mw of the pullulan produced by A. melanogenum P16 grown in the initial medium was only 3.47×105 and a pullulan titer was 65.3g/L.
28465177	1	65	theme	pullulan	317:324	arg1	titer					326:330	a pullulan titer	315:330	a pullulan titer	315:330	After the compositional change of a pullulan production medium, a molecular weight (Mw) of the pullulan produced by Aureobasidium melanogenum P16 was 2.32×106 and a pullulan titer was 44.4g/L while a Mw of the pullulan produced by A. melanogenum P16 grown in the initial medium was only 3.47×105 and a pullulan titer was 65.3g/L.
28465177	5	66	theme	high	1417:1420	arg1	pullulan					1425:1432	the high Mw pullulan	1413:1432	the high Mw pullulan	1413:1432	Therefore, both of the high Mw pullulan and oligosaccharides with high levels were produced by the yeast strain P16.
27131730	1	0	theme	anion-exchange	341:354	arg1	chromatography					356:369	DEAE-cellulose anion-exchange chromatography	326:369	DEAE-cellulose anion-exchange chromatography	326:369	In this report, a water-soluble polysaccharide was obtained from the dried stems of Dendrobium officinale Kimura et Migo by hot-water (70-75°C) extraction and 85% ethanol precipitation, and successively purification by DEAE-cellulose anion-exchange chromatography and gel-permeation chromatography.
27131730	6	1	theme	DPPH	1162:1165	arg1	radical					1168:1174	1,1-diphenyl-2-picrylhydrazyl (DPPH) radical	1131:1174	1,1-diphenyl-2-picrylhydrazyl (DPPH) radical	1131:1174	The antioxidant activities of the polysaccharide in vitro assay indicate that DOP has good scavenging activity of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, higher scavenging activity of hydroxyl radical, and metal chelating activities.
27131730	6	2	theme	polysaccharide	1051:1064	arg1	assay					1075:1079	the polysaccharide in vitro assay	1047:1079	the polysaccharide in vitro assay	1047:1079	The antioxidant activities of the polysaccharide in vitro assay indicate that DOP has good scavenging activity of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, higher scavenging activity of hydroxyl radical, and metal chelating activities.
27131730	0	3	theme	Dendrobium	84:93	arg1	officinale					95:104	Dendrobium officinale	84:104	Dendrobium officinale	84:104	Chemical properties and antioxidant activity of a water-soluble polysaccharide from Dendrobium officinale.
27131730	0	4	from	properties	9:18	arg1	officinale					95:104	Dendrobium officinale	84:104	Dendrobium officinale	84:104	Chemical properties and antioxidant activity of a water-soluble polysaccharide from Dendrobium officinale.
27131730	5	5	theme	polymerization	1001:1014	arg1	degrees					990:996	high degrees	985:996	high degrees of polymerization	985:1014	Atomic force microscope shows that DOP mainly exists as rod-shaped chains, supporting high degrees of polymerization.
27131730	1	6	theme	gel-permeation	375:388	arg1	chromatography					390:403	gel-permeation chromatography	375:403	gel-permeation chromatography	375:403	In this report, a water-soluble polysaccharide was obtained from the dried stems of Dendrobium officinale Kimura et Migo by hot-water (70-75°C) extraction and 85% ethanol precipitation, and successively purification by DEAE-cellulose anion-exchange chromatography and gel-permeation chromatography.
27131730	6	7	theme	chelating	1235:1243	arg1	activities					1245:1254	metal chelating activities	1229:1254	metal chelating activities	1229:1254	The antioxidant activities of the polysaccharide in vitro assay indicate that DOP has good scavenging activity of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, higher scavenging activity of hydroxyl radical, and metal chelating activities.
27131730	4	8	theme	mannose	756:762	arg1	predominance					740:751	the predominance	736:751	the predominance of mannose and glucose	736:774	Periodate oxidation-smith degradation and 1D and 2D NMR spectroscopy analysis suggest the predominance of mannose and glucose, and it contains a 2-O-acetylglucomannan and (1→4)-linked-β-d-mannopyranosyl and (1→4)-linked-β-d-glucopyranosyl residues.
27131730	1	9	theme	85	266:267	arg1	%					268:268	%	268:268	%	268:268	In this report, a water-soluble polysaccharide was obtained from the dried stems of Dendrobium officinale Kimura et Migo by hot-water (70-75°C) extraction and 85% ethanol precipitation, and successively purification by DEAE-cellulose anion-exchange chromatography and gel-permeation chromatography.
27131730	6	10	dep	in	1066:1067	arg1	vitro					1069:1073	vitro	1069:1073	vitro	1069:1073	The antioxidant activities of the polysaccharide in vitro assay indicate that DOP has good scavenging activity of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, higher scavenging activity of hydroxyl radical, and metal chelating activities.
27131730	4	11	theme	-linked-β-d-glucopyranosyl	862:887	arg1	residues					889:896	a 2-O-acetylglucomannan and (1→4)-linked-β-d-mannopyranosyl and (1→4)-linked-β-d-glucopyranosyl residues	793:896	a 2-O-acetylglucomannan and (1→4)-linked-β-d-mannopyranosyl and (1→4)-linked-β-d-glucopyranosyl residues	793:896	Periodate oxidation-smith degradation and 1D and 2D NMR spectroscopy analysis suggest the predominance of mannose and glucose, and it contains a 2-O-acetylglucomannan and (1→4)-linked-β-d-mannopyranosyl and (1→4)-linked-β-d-glucopyranosyl residues.
27131730	6	12	theme	1,1-diphenyl-2-picrylhydrazyl	1131:1159	arg1	radical					1168:1174	1,1-diphenyl-2-picrylhydrazyl (DPPH) radical	1131:1174	1,1-diphenyl-2-picrylhydrazyl (DPPH) radical	1131:1174	The antioxidant activities of the polysaccharide in vitro assay indicate that DOP has good scavenging activity of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, higher scavenging activity of hydroxyl radical, and metal chelating activities.
27131730	4	13	theme	2-O-acetylglucomannan	795:815	arg1	residues					889:896	a 2-O-acetylglucomannan and (1→4)-linked-β-d-mannopyranosyl and (1→4)-linked-β-d-glucopyranosyl residues	793:896	a 2-O-acetylglucomannan and (1→4)-linked-β-d-mannopyranosyl and (1→4)-linked-β-d-glucopyranosyl residues	793:896	Periodate oxidation-smith degradation and 1D and 2D NMR spectroscopy analysis suggest the predominance of mannose and glucose, and it contains a 2-O-acetylglucomannan and (1→4)-linked-β-d-mannopyranosyl and (1→4)-linked-β-d-glucopyranosyl residues.
27131730	3	14	from	ratio	624:628	arg1	trace					586:590	a trace	584:590	a trace of galacturonic acid in a molar ratio of 6.2:2.3:2.1:0.1	584:647	Monosaccharide composition analysis reveals that DOP is composed of mannose, glucose, and arabinose with a trace of galacturonic acid in a molar ratio of 6.2:2.3:2.1:0.1.
27131730	1	15	theme	dried	176:180	arg1	stems					182:186	the dried stems	172:186	the dried stems of Dendrobium officinale Kimura	172:218	In this report, a water-soluble polysaccharide was obtained from the dried stems of Dendrobium officinale Kimura et Migo by hot-water (70-75°C) extraction and 85% ethanol precipitation, and successively purification by DEAE-cellulose anion-exchange chromatography and gel-permeation chromatography.
27131730	6	16	contain	has	1099:1101	arg2	activities					1245:1254	metal chelating activities	1229:1254	metal chelating activities	1229:1254	The antioxidant activities of the polysaccharide in vitro assay indicate that DOP has good scavenging activity of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, higher scavenging activity of hydroxyl radical, and metal chelating activities.
27131730	6	16	contain	has	1099:1101	arg2	activity					1195:1202	higher scavenging activity	1177:1202	higher scavenging activity of hydroxyl radical	1177:1222	The antioxidant activities of the polysaccharide in vitro assay indicate that DOP has good scavenging activity of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, higher scavenging activity of hydroxyl radical, and metal chelating activities.
27131730	6	16	contain	has	1099:1101	arg2	activity					1119:1126	good scavenging activity	1103:1126	good scavenging activity of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical	1103:1174	The antioxidant activities of the polysaccharide in vitro assay indicate that DOP has good scavenging activity of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, higher scavenging activity of hydroxyl radical, and metal chelating activities.
27131730	6	16	contain	has	1099:1101	arg1	DOP					1095:1097	DOP	1095:1097	DOP	1095:1097	The antioxidant activities of the polysaccharide in vitro assay indicate that DOP has good scavenging activity of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, higher scavenging activity of hydroxyl radical, and metal chelating activities.
27131730	6	17	theme	higher	1177:1182	arg1	activity					1195:1202	higher scavenging activity	1177:1202	higher scavenging activity of hydroxyl radical	1177:1222	The antioxidant activities of the polysaccharide in vitro assay indicate that DOP has good scavenging activity of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, higher scavenging activity of hydroxyl radical, and metal chelating activities.
27131730	6	18	theme	assay	1075:1079	arg1	activities					1033:1042	The antioxidant activities	1017:1042	The antioxidant activities of the polysaccharide in vitro assay	1017:1079	The antioxidant activities of the polysaccharide in vitro assay indicate that DOP has good scavenging activity of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, higher scavenging activity of hydroxyl radical, and metal chelating activities.
27131730	5	19	theme	force	906:910	arg1	microscope					912:921	Atomic force microscope	899:921	Atomic force microscope	899:921	Atomic force microscope shows that DOP mainly exists as rod-shaped chains, supporting high degrees of polymerization.
27131730	2	20	theme	8500Da	471:476	arg1	weight					461:466	a molecular weight	449:466	a molecular weight of 8500Da	449:476	The D. officinale polysaccharide (DOP) has a molecular weight of 8500Da.
27131730	0	21	from	activity	36:43	arg1	officinale					95:104	Dendrobium officinale	84:104	Dendrobium officinale	84:104	Chemical properties and antioxidant activity of a water-soluble polysaccharide from Dendrobium officinale.
27131730	6	22	theme	antioxidant	1021:1031	arg1	activities					1033:1042	The antioxidant activities	1017:1042	The antioxidant activities of the polysaccharide in vitro assay	1017:1079	The antioxidant activities of the polysaccharide in vitro assay indicate that DOP has good scavenging activity of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, higher scavenging activity of hydroxyl radical, and metal chelating activities.
27131730	4	23	theme	glucose	768:774	arg1	predominance					740:751	the predominance	736:751	the predominance of mannose and glucose	736:774	Periodate oxidation-smith degradation and 1D and 2D NMR spectroscopy analysis suggest the predominance of mannose and glucose, and it contains a 2-O-acetylglucomannan and (1→4)-linked-β-d-mannopyranosyl and (1→4)-linked-β-d-glucopyranosyl residues.
27131730	2	24	theme	officinale	413:422	arg1	polysaccharide					424:437	The D. officinale polysaccharide	406:437	The D. officinale polysaccharide (DOP)	406:443	The D. officinale polysaccharide (DOP) has a molecular weight of 8500Da.
27131730	2	24	theme	officinale	413:422	arg1	DOP					440:442	DOP	440:442	DOP	440:442	The D. officinale polysaccharide (DOP) has a molecular weight of 8500Da.
27131730	0	25	theme	Chemical	0:7	arg1	properties					9:18	Chemical properties	0:18	Chemical properties	0:18	Chemical properties and antioxidant activity of a water-soluble polysaccharide from Dendrobium officinale.
27131730	1	26	theme	%	268:268	arg1	precipitation					278:290	85% ethanol precipitation	266:290	85% ethanol precipitation	266:290	In this report, a water-soluble polysaccharide was obtained from the dried stems of Dendrobium officinale Kimura et Migo by hot-water (70-75°C) extraction and 85% ethanol precipitation, and successively purification by DEAE-cellulose anion-exchange chromatography and gel-permeation chromatography.
27131730	6	27	theme	in	1066:1067	arg1	assay					1075:1079	the polysaccharide in vitro assay	1047:1079	the polysaccharide in vitro assay	1047:1079	The antioxidant activities of the polysaccharide in vitro assay indicate that DOP has good scavenging activity of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, higher scavenging activity of hydroxyl radical, and metal chelating activities.
27131730	6	28	theme	good	1103:1106	arg1	activity					1119:1126	good scavenging activity	1103:1126	good scavenging activity of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical	1103:1174	The antioxidant activities of the polysaccharide in vitro assay indicate that DOP has good scavenging activity of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, higher scavenging activity of hydroxyl radical, and metal chelating activities.
27131730	5	29	theme	high	985:988	arg1	degrees					990:996	high degrees	985:996	high degrees of polymerization	985:1014	Atomic force microscope shows that DOP mainly exists as rod-shaped chains, supporting high degrees of polymerization.
27131730	1	30	theme	Dendrobium	191:200	arg1	Kimura					213:218	Dendrobium officinale Kimura	191:218	Dendrobium officinale Kimura	191:218	In this report, a water-soluble polysaccharide was obtained from the dried stems of Dendrobium officinale Kimura et Migo by hot-water (70-75°C) extraction and 85% ethanol precipitation, and successively purification by DEAE-cellulose anion-exchange chromatography and gel-permeation chromatography.
27131730	0	31	theme	antioxidant	24:34	arg1	activity					36:43	antioxidant activity	24:43	antioxidant activity	24:43	Chemical properties and antioxidant activity of a water-soluble polysaccharide from Dendrobium officinale.
27131730	1	32	theme	ethanol	270:276	arg1	precipitation					278:290	85% ethanol precipitation	266:290	85% ethanol precipitation	266:290	In this report, a water-soluble polysaccharide was obtained from the dried stems of Dendrobium officinale Kimura et Migo by hot-water (70-75°C) extraction and 85% ethanol precipitation, and successively purification by DEAE-cellulose anion-exchange chromatography and gel-permeation chromatography.
27131730	6	33	theme	hydroxyl	1207:1214	arg1	radical					1216:1222	hydroxyl radical	1207:1222	hydroxyl radical	1207:1222	The antioxidant activities of the polysaccharide in vitro assay indicate that DOP has good scavenging activity of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, higher scavenging activity of hydroxyl radical, and metal chelating activities.
27131730	3	34	from	acid	608:611	arg1	ratio					624:628	a molar ratio	616:628	a molar ratio of 6.2:2.3:2.1:0.1	616:647	Monosaccharide composition analysis reveals that DOP is composed of mannose, glucose, and arabinose with a trace of galacturonic acid in a molar ratio of 6.2:2.3:2.1:0.1.
27131730	1	35	theme	officinale	202:211	arg1	Kimura					213:218	Dendrobium officinale Kimura	191:218	Dendrobium officinale Kimura	191:218	In this report, a water-soluble polysaccharide was obtained from the dried stems of Dendrobium officinale Kimura et Migo by hot-water (70-75°C) extraction and 85% ethanol precipitation, and successively purification by DEAE-cellulose anion-exchange chromatography and gel-permeation chromatography.
27131730	2	36	theme	molecular	451:459	arg1	weight					461:466	a molecular weight	449:466	a molecular weight of 8500Da	449:476	The D. officinale polysaccharide (DOP) has a molecular weight of 8500Da.
27131730	6	37	theme	metal	1229:1233	arg1	activities					1245:1254	metal chelating activities	1229:1254	metal chelating activities	1229:1254	The antioxidant activities of the polysaccharide in vitro assay indicate that DOP has good scavenging activity of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, higher scavenging activity of hydroxyl radical, and metal chelating activities.
27131730	0	38	from	officinale	95:104	arg1	polysaccharide					64:77	a water-soluble polysaccharide	48:77	a water-soluble polysaccharide from Dendrobium officinale	48:104	Chemical properties and antioxidant activity of a water-soluble polysaccharide from Dendrobium officinale.
27131730	0	38	from	officinale	95:104	arg1	properties					9:18	Chemical properties	0:18	Chemical properties	0:18	Chemical properties and antioxidant activity of a water-soluble polysaccharide from Dendrobium officinale.
27131730	0	38	from	officinale	95:104	arg1	activity					36:43	antioxidant activity	24:43	antioxidant activity	24:43	Chemical properties and antioxidant activity of a water-soluble polysaccharide from Dendrobium officinale.
27131730	1	39	theme	Kimura	213:218	arg1	stems					182:186	the dried stems	172:186	the dried stems of Dendrobium officinale Kimura	172:218	In this report, a water-soluble polysaccharide was obtained from the dried stems of Dendrobium officinale Kimura et Migo by hot-water (70-75°C) extraction and 85% ethanol precipitation, and successively purification by DEAE-cellulose anion-exchange chromatography and gel-permeation chromatography.
27131730	6	40	theme	scavenging	1184:1193	arg1	activity					1195:1202	higher scavenging activity	1177:1202	higher scavenging activity of hydroxyl radical	1177:1222	The antioxidant activities of the polysaccharide in vitro assay indicate that DOP has good scavenging activity of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, higher scavenging activity of hydroxyl radical, and metal chelating activities.
27131730	4	41	dep	degradation	676:686	arg1	analysis					719:726	NMR spectroscopy analysis	702:726	NMR spectroscopy analysis	702:726	Periodate oxidation-smith degradation and 1D and 2D NMR spectroscopy analysis suggest the predominance of mannose and glucose, and it contains a 2-O-acetylglucomannan and (1→4)-linked-β-d-mannopyranosyl and (1→4)-linked-β-d-glucopyranosyl residues.
27131730	2	42	theme	D.	410:411	arg1	polysaccharide					424:437	The D. officinale polysaccharide	406:437	The D. officinale polysaccharide (DOP)	406:443	The D. officinale polysaccharide (DOP) has a molecular weight of 8500Da.
27131730	2	42	theme	D.	410:411	arg1	DOP					440:442	DOP	440:442	DOP	440:442	The D. officinale polysaccharide (DOP) has a molecular weight of 8500Da.
27131730	5	43	theme	Atomic	899:904	arg1	microscope					912:921	Atomic force microscope	899:921	Atomic force microscope	899:921	Atomic force microscope shows that DOP mainly exists as rod-shaped chains, supporting high degrees of polymerization.
27131730	4	44	theme	-linked-β-d-mannopyranosyl	826:851	arg1	residues					889:896	a 2-O-acetylglucomannan and (1→4)-linked-β-d-mannopyranosyl and (1→4)-linked-β-d-glucopyranosyl residues	793:896	a 2-O-acetylglucomannan and (1→4)-linked-β-d-mannopyranosyl and (1→4)-linked-β-d-glucopyranosyl residues	793:896	Periodate oxidation-smith degradation and 1D and 2D NMR spectroscopy analysis suggest the predominance of mannose and glucose, and it contains a 2-O-acetylglucomannan and (1→4)-linked-β-d-mannopyranosyl and (1→4)-linked-β-d-glucopyranosyl residues.
27131730	3	45	theme	Monosaccharide	479:492	arg1	analysis					506:513	Monosaccharide composition analysis	479:513	Monosaccharide composition analysis	479:513	Monosaccharide composition analysis reveals that DOP is composed of mannose, glucose, and arabinose with a trace of galacturonic acid in a molar ratio of 6.2:2.3:2.1:0.1.
27131730	0	46	theme	water-soluble	50:62	arg1	polysaccharide					64:77	a water-soluble polysaccharide	48:77	a water-soluble polysaccharide from Dendrobium officinale	48:104	Chemical properties and antioxidant activity of a water-soluble polysaccharide from Dendrobium officinale.
27131730	1	47	theme	successively	297:308	arg1	purification					310:321	successively purification	297:321	successively purification	297:321	In this report, a water-soluble polysaccharide was obtained from the dried stems of Dendrobium officinale Kimura et Migo by hot-water (70-75°C) extraction and 85% ethanol precipitation, and successively purification by DEAE-cellulose anion-exchange chromatography and gel-permeation chromatography.
27131730	3	48	theme	galacturonic	595:606	arg1	acid					608:611	galacturonic acid	595:611	galacturonic acid in a molar ratio of 6.2:2.3:2.1:0.1	595:647	Monosaccharide composition analysis reveals that DOP is composed of mannose, glucose, and arabinose with a trace of galacturonic acid in a molar ratio of 6.2:2.3:2.1:0.1.
27131730	6	49	theme	radical	1168:1174	arg1	activity					1119:1126	good scavenging activity	1103:1126	good scavenging activity of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical	1103:1174	The antioxidant activities of the polysaccharide in vitro assay indicate that DOP has good scavenging activity of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, higher scavenging activity of hydroxyl radical, and metal chelating activities.
27131730	6	49	theme	radical	1168:1174	arg1	activities					1245:1254	metal chelating activities	1229:1254	metal chelating activities	1229:1254	The antioxidant activities of the polysaccharide in vitro assay indicate that DOP has good scavenging activity of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, higher scavenging activity of hydroxyl radical, and metal chelating activities.
27131730	6	49	theme	radical	1168:1174	arg1	activity					1195:1202	higher scavenging activity	1177:1202	higher scavenging activity of hydroxyl radical	1177:1222	The antioxidant activities of the polysaccharide in vitro assay indicate that DOP has good scavenging activity of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, higher scavenging activity of hydroxyl radical, and metal chelating activities.
27131730	6	50	theme	scavenging	1108:1117	arg1	activity					1119:1126	good scavenging activity	1103:1126	good scavenging activity of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical	1103:1174	The antioxidant activities of the polysaccharide in vitro assay indicate that DOP has good scavenging activity of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, higher scavenging activity of hydroxyl radical, and metal chelating activities.
27131730	3	51	theme	acid	608:611	arg1	trace					586:590	a trace	584:590	a trace of galacturonic acid in a molar ratio of 6.2:2.3:2.1:0.1	584:647	Monosaccharide composition analysis reveals that DOP is composed of mannose, glucose, and arabinose with a trace of galacturonic acid in a molar ratio of 6.2:2.3:2.1:0.1.
27131730	5	52	theme	rod-shaped	955:964	arg1	chains					966:971	rod-shaped chains	955:971	rod-shaped chains	955:971	Atomic force microscope shows that DOP mainly exists as rod-shaped chains, supporting high degrees of polymerization.
27131730	4	53	contain	contains	784:791	arg1	it					781:782	it	781:782	it	781:782	Periodate oxidation-smith degradation and 1D and 2D NMR spectroscopy analysis suggest the predominance of mannose and glucose, and it contains a 2-O-acetylglucomannan and (1→4)-linked-β-d-mannopyranosyl and (1→4)-linked-β-d-glucopyranosyl residues.
27131730	4	53	contain	contains	784:791	arg2	residues					889:896	a 2-O-acetylglucomannan and (1→4)-linked-β-d-mannopyranosyl and (1→4)-linked-β-d-glucopyranosyl residues	793:896	a 2-O-acetylglucomannan and (1→4)-linked-β-d-mannopyranosyl and (1→4)-linked-β-d-glucopyranosyl residues	793:896	Periodate oxidation-smith degradation and 1D and 2D NMR spectroscopy analysis suggest the predominance of mannose and glucose, and it contains a 2-O-acetylglucomannan and (1→4)-linked-β-d-mannopyranosyl and (1→4)-linked-β-d-glucopyranosyl residues.
27131730	1	54	theme	water-soluble	125:137	arg1	polysaccharide					139:152	a water-soluble polysaccharide	123:152	a water-soluble polysaccharide	123:152	In this report, a water-soluble polysaccharide was obtained from the dried stems of Dendrobium officinale Kimura et Migo by hot-water (70-75°C) extraction and 85% ethanol precipitation, and successively purification by DEAE-cellulose anion-exchange chromatography and gel-permeation chromatography.
27131730	2	55	contain	has	445:447	arg1	polysaccharide					424:437	The D. officinale polysaccharide	406:437	The D. officinale polysaccharide (DOP)	406:443	The D. officinale polysaccharide (DOP) has a molecular weight of 8500Da.
27131730	2	55	contain	has	445:447	arg1	DOP					440:442	DOP	440:442	DOP	440:442	The D. officinale polysaccharide (DOP) has a molecular weight of 8500Da.
27131730	2	55	contain	has	445:447	arg2	weight					461:466	a molecular weight	449:466	a molecular weight of 8500Da	449:476	The D. officinale polysaccharide (DOP) has a molecular weight of 8500Da.
27131730	3	56	theme	6.2:2.3:2.1:0.1	633:647	arg1	ratio					624:628	a molar ratio	616:628	a molar ratio of 6.2:2.3:2.1:0.1	616:647	Monosaccharide composition analysis reveals that DOP is composed of mannose, glucose, and arabinose with a trace of galacturonic acid in a molar ratio of 6.2:2.3:2.1:0.1.
27131730	3	57	from	trace	586:590	arg1	ratio					624:628	a molar ratio	616:628	a molar ratio of 6.2:2.3:2.1:0.1	616:647	Monosaccharide composition analysis reveals that DOP is composed of mannose, glucose, and arabinose with a trace of galacturonic acid in a molar ratio of 6.2:2.3:2.1:0.1.
27131730	1	58	theme	hot-water	231:239	arg1	extraction					251:260	hot-water (70-75°C) extraction	231:260	hot-water (70-75°C) extraction	231:260	In this report, a water-soluble polysaccharide was obtained from the dried stems of Dendrobium officinale Kimura et Migo by hot-water (70-75°C) extraction and 85% ethanol precipitation, and successively purification by DEAE-cellulose anion-exchange chromatography and gel-permeation chromatography.
27131730	4	59	theme	oxidation-smith	660:674	arg1	degradation					676:686	Periodate oxidation-smith degradation	650:686	Periodate oxidation-smith degradation	650:686	Periodate oxidation-smith degradation and 1D and 2D NMR spectroscopy analysis suggest the predominance of mannose and glucose, and it contains a 2-O-acetylglucomannan and (1→4)-linked-β-d-mannopyranosyl and (1→4)-linked-β-d-glucopyranosyl residues.
27131730	3	60	theme	molar	618:622	arg1	ratio					624:628	a molar ratio	616:628	a molar ratio of 6.2:2.3:2.1:0.1	616:647	Monosaccharide composition analysis reveals that DOP is composed of mannose, glucose, and arabinose with a trace of galacturonic acid in a molar ratio of 6.2:2.3:2.1:0.1.
27131730	3	61	theme	composition	494:504	arg1	analysis					506:513	Monosaccharide composition analysis	479:513	Monosaccharide composition analysis	479:513	Monosaccharide composition analysis reveals that DOP is composed of mannose, glucose, and arabinose with a trace of galacturonic acid in a molar ratio of 6.2:2.3:2.1:0.1.
27131730	4	62	theme	spectroscopy	706:717	arg1	analysis					719:726	NMR spectroscopy analysis	702:726	NMR spectroscopy analysis	702:726	Periodate oxidation-smith degradation and 1D and 2D NMR spectroscopy analysis suggest the predominance of mannose and glucose, and it contains a 2-O-acetylglucomannan and (1→4)-linked-β-d-mannopyranosyl and (1→4)-linked-β-d-glucopyranosyl residues.
27131730	6	63	theme	radical	1216:1222	arg1	activity					1119:1126	good scavenging activity	1103:1126	good scavenging activity of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical	1103:1174	The antioxidant activities of the polysaccharide in vitro assay indicate that DOP has good scavenging activity of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, higher scavenging activity of hydroxyl radical, and metal chelating activities.
27131730	6	63	theme	radical	1216:1222	arg1	activities					1245:1254	metal chelating activities	1229:1254	metal chelating activities	1229:1254	The antioxidant activities of the polysaccharide in vitro assay indicate that DOP has good scavenging activity of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, higher scavenging activity of hydroxyl radical, and metal chelating activities.
27131730	6	63	theme	radical	1216:1222	arg1	activity					1195:1202	higher scavenging activity	1177:1202	higher scavenging activity of hydroxyl radical	1177:1222	The antioxidant activities of the polysaccharide in vitro assay indicate that DOP has good scavenging activity of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, higher scavenging activity of hydroxyl radical, and metal chelating activities.
27131730	0	64	theme	polysaccharide	64:77	arg1	properties					9:18	Chemical properties	0:18	Chemical properties	0:18	Chemical properties and antioxidant activity of a water-soluble polysaccharide from Dendrobium officinale.
27131730	0	64	theme	polysaccharide	64:77	arg1	activity					36:43	antioxidant activity	24:43	antioxidant activity	24:43	Chemical properties and antioxidant activity of a water-soluble polysaccharide from Dendrobium officinale.
27131730	1	65	theme	DEAE-cellulose	326:339	arg1	chromatography					356:369	DEAE-cellulose anion-exchange chromatography	326:369	DEAE-cellulose anion-exchange chromatography	326:369	In this report, a water-soluble polysaccharide was obtained from the dried stems of Dendrobium officinale Kimura et Migo by hot-water (70-75°C) extraction and 85% ethanol precipitation, and successively purification by DEAE-cellulose anion-exchange chromatography and gel-permeation chromatography.
27131730	4	66	theme	Periodate	650:658	arg1	degradation					676:686	Periodate oxidation-smith degradation	650:686	Periodate oxidation-smith degradation	650:686	Periodate oxidation-smith degradation and 1D and 2D NMR spectroscopy analysis suggest the predominance of mannose and glucose, and it contains a 2-O-acetylglucomannan and (1→4)-linked-β-d-mannopyranosyl and (1→4)-linked-β-d-glucopyranosyl residues.
27131730	4	67	theme	NMR	702:704	arg1	analysis					719:726	NMR spectroscopy analysis	702:726	NMR spectroscopy analysis	702:726	Periodate oxidation-smith degradation and 1D and 2D NMR spectroscopy analysis suggest the predominance of mannose and glucose, and it contains a 2-O-acetylglucomannan and (1→4)-linked-β-d-mannopyranosyl and (1→4)-linked-β-d-glucopyranosyl residues.
27131730	1	68	theme	70-75°C	242:248	arg1	extraction					251:260	hot-water (70-75°C) extraction	231:260	hot-water (70-75°C) extraction	231:260	In this report, a water-soluble polysaccharide was obtained from the dried stems of Dendrobium officinale Kimura et Migo by hot-water (70-75°C) extraction and 85% ethanol precipitation, and successively purification by DEAE-cellulose anion-exchange chromatography and gel-permeation chromatography.
27651751	0	0	theme	endurance	76:84	arg1	exercise					86:93	endurance exercise	76:93	endurance exercise	76:93	Intestinal adaptations to a combination of different diets with and without endurance exercise.
27651751	8	1	theme	mucosa	1427:1432	arg1	percentage					1413:1422	the percentage	1409:1422	the percentage of mucosa	1409:1432	Yet, the percentage of mucosa did not differ among groups.
27651751	6	2	dep	wider	1248:1252	arg1	P < 0.05					1255:1262	P < 0.05	1255:1262	P < 0.05	1255:1262	The Daniel Fast rats had longer and heavier intestines with deeper crypts with villi that were wider (P < 0.05), but not taller.
27651751	6	3	theme	heavier	1189:1195	arg1	intestines					1197:1206	longer and heavier intestines	1178:1206	longer and heavier intestines	1178:1206	The Daniel Fast rats had longer and heavier intestines with deeper crypts with villi that were wider (P < 0.05), but not taller.
27651751	2	4	dep	amount	303:308	arg1	the					299:301	the	299:301	the	299:301	Although the intestine can adapt to changes in the amount and composition of dietary inputs, the responses to the combination of endurance exercise and diet are poorly understood.
27651751	10	5	theme	small	1680:1684	arg1	intestine					1686:1694	the small intestine	1676:1694	the small intestine	1676:1694	CONCLUSIONS These findings indicate the structural responses of the small intestine to a vegan style diet are modified by exercise, but without altering the capacities of the brush border membrane to digest and absorb carbohydrates.
27651751	4	6	theme	variation	939:947	arg1	analysis					927:934	analysis	927:934	analysis of variation	927:947	The influences of diet and exercise, alone and in combination, were analyzed by analysis of variation.
27651751	3	7	dep	period	821:826	arg1	n = 7					829:833	n = 7	829:833	n = 7 per group	829:843	METHODS We evaluated small intestinal dimensions and mucosal architecture and calculated the capacities of the entire small intestine to digest maltose and maltodextrin and absorb glucose in response to two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group).
27651751	7	8	contain	had	1338:1340	arg2	intestines					1362:1371	shorter and lighter intestines	1342:1371	shorter and lighter intestines	1342:1371	Despite increased energetic demands, the exercised rats had shorter and lighter intestines with shorter villi (P < 0.05).
27651751	7	8	contain	had	1338:1340	arg1	rats					1333:1336	the exercised rats	1319:1336	the exercised rats	1319:1336	Despite increased energetic demands, the exercised rats had shorter and lighter intestines with shorter villi (P < 0.05).
27651751	6	9	with	crypts	1220:1225	arg1	villi					1232:1236	villi	1232:1236	villi that were wider (P < 0.05), but not taller	1232:1279	The Daniel Fast rats had longer and heavier intestines with deeper crypts with villi that were wider (P < 0.05), but not taller.
27651751	5	10	theme	similar	1054:1060	arg1	response					1062:1069	a similar response	1052:1069	a similar response for the sedentary compared with the exercised rats in each diet group (P < 0.05)	1052:1150	RESULTS Rats fed the western diet gained more weight (P < 0.05) due to more fat mass (P < 0.05), with a similar response for the sedentary compared with the exercised rats in each diet group (P < 0.05).
27651751	9	11	theme	Total	1463:1467	arg1	activities					1486:1495	Total small intestinal activities	1463:1495	Total small intestinal activities for maltase and α-glucoamylase	1463:1526	Total small intestinal activities for maltase and α-glucoamylase, and capacities for glucose absorption were similar regardless of diet or exercise.
27651751	10	12	theme	membrane	1800:1807	arg1	capacities					1769:1778	the capacities	1765:1778	the capacities of the brush border membrane to digest and absorb carbohydrates	1765:1842	CONCLUSIONS These findings indicate the structural responses of the small intestine to a vegan style diet are modified by exercise, but without altering the capacities of the brush border membrane to digest and absorb carbohydrates.
27651751	10	13	dep	CONCLUSIONS	1612:1622	arg1	indicate					1639:1646	indicate	1639:1646	indicate the structural responses of the small intestine to a vegan style diet are modified by exercise, but without altering the capacities of the brush border membrane to digest and absorb carbohydrates	1639:1842	CONCLUSIONS These findings indicate the structural responses of the small intestine to a vegan style diet are modified by exercise, but without altering the capacities of the brush border membrane to digest and absorb carbohydrates.
27651751	9	14	theme	intestinal	1475:1484	arg1	activities					1486:1495	Total small intestinal activities	1463:1495	Total small intestinal activities for maltase and α-glucoamylase	1463:1526	Total small intestinal activities for maltase and α-glucoamylase, and capacities for glucose absorption were similar regardless of diet or exercise.
27651751	1	15	theme	diet	137:140	arg1	regimens					142:149	diet regimens	137:149	diet regimens that will improve performance and decrease gastrointestinal disturbances during training and events	137:249	BACKGROUND Endurance athletes search for diet regimens that will improve performance and decrease gastrointestinal disturbances during training and events.
27651751	7	16	theme	shorter	1342:1348	arg1	intestines					1362:1371	shorter and lighter intestines	1342:1371	shorter and lighter intestines	1342:1371	Despite increased energetic demands, the exercised rats had shorter and lighter intestines with shorter villi (P < 0.05).
27651751	6	17	theme	Fast	1164:1167	arg1	rats					1169:1172	The Daniel Fast rats	1153:1172	The Daniel Fast rats	1153:1172	The Daniel Fast rats had longer and heavier intestines with deeper crypts with villi that were wider (P < 0.05), but not taller.
27651751	5	18	theme	diet	1130:1133	arg1	P < 0.05					1142:1149	P < 0.05	1142:1149	P < 0.05	1142:1149	RESULTS Rats fed the western diet gained more weight (P < 0.05) due to more fat mass (P < 0.05), with a similar response for the sedentary compared with the exercised rats in each diet group (P < 0.05).
27651751	5	18	theme	diet	1130:1133	arg1	group					1135:1139	each diet group	1125:1139	each diet group (P < 0.05)	1125:1150	RESULTS Rats fed the western diet gained more weight (P < 0.05) due to more fat mass (P < 0.05), with a similar response for the sedentary compared with the exercised rats in each diet group (P < 0.05).
27651751	3	19	theme	small	550:554	arg1	intestine					556:564	the entire small intestine	539:564	the entire small intestine	539:564	METHODS We evaluated small intestinal dimensions and mucosal architecture and calculated the capacities of the entire small intestine to digest maltose and maltodextrin and absorb glucose in response to two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group).
27651751	6	20	contain	had	1174:1176	arg2	intestines					1197:1206	longer and heavier intestines	1178:1206	longer and heavier intestines	1178:1206	The Daniel Fast rats had longer and heavier intestines with deeper crypts with villi that were wider (P < 0.05), but not taller.
27651751	6	20	contain	had	1174:1176	arg1	rats					1169:1172	The Daniel Fast rats	1153:1172	The Daniel Fast rats	1153:1172	The Daniel Fast rats had longer and heavier intestines with deeper crypts with villi that were wider (P < 0.05), but not taller.
27651751	2	21	theme	diet	404:407	arg1	combination					366:376	the combination	362:376	the combination of endurance exercise and diet	362:407	Although the intestine can adapt to changes in the amount and composition of dietary inputs, the responses to the combination of endurance exercise and diet are poorly understood.
27651751	3	22	theme	13 week	813:819	arg1	period					821:826	a 13 week period	811:826	a 13 week period (n = 7 per group)	811:844	METHODS We evaluated small intestinal dimensions and mucosal architecture and calculated the capacities of the entire small intestine to digest maltose and maltodextrin and absorb glucose in response to two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group).
27651751	3	23	theme	vegan	705:709	arg1	types					654:658	two different diet types	635:658	two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group)	635:844	METHODS We evaluated small intestinal dimensions and mucosal architecture and calculated the capacities of the entire small intestine to digest maltose and maltodextrin and absorb glucose in response to two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group).
27651751	3	23	theme	vegan	705:709	arg1	diet					717:720	a vegan style diet	703:720	a vegan style diet	703:720	METHODS We evaluated small intestinal dimensions and mucosal architecture and calculated the capacities of the entire small intestine to digest maltose and maltodextrin and absorb glucose in response to two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group).
27651751	6	24	theme	Daniel	1157:1162	arg1	rats					1169:1172	The Daniel Fast rats	1153:1172	The Daniel Fast rats	1153:1172	The Daniel Fast rats had longer and heavier intestines with deeper crypts with villi that were wider (P < 0.05), but not taller.
27651751	3	25	theme	intestine	556:564	arg1	capacities					525:534	the capacities	521:534	the capacities of the entire small intestine to digest maltose and maltodextrin and absorb glucose in response to two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group)	521:844	METHODS We evaluated small intestinal dimensions and mucosal architecture and calculated the capacities of the entire small intestine to digest maltose and maltodextrin and absorb glucose in response to two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group).
27651751	3	26	theme	small	453:457	arg1	dimensions					470:479	small intestinal dimensions	453:479	small intestinal dimensions	453:479	METHODS We evaluated small intestinal dimensions and mucosal architecture and calculated the capacities of the entire small intestine to digest maltose and maltodextrin and absorb glucose in response to two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group).
27651751	3	27	theme	style	711:715	arg1	types					654:658	two different diet types	635:658	two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group)	635:844	METHODS We evaluated small intestinal dimensions and mucosal architecture and calculated the capacities of the entire small intestine to digest maltose and maltodextrin and absorb glucose in response to two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group).
27651751	3	27	theme	style	711:715	arg1	diet					717:720	a vegan style diet	703:720	a vegan style diet	703:720	METHODS We evaluated small intestinal dimensions and mucosal architecture and calculated the capacities of the entire small intestine to digest maltose and maltodextrin and absorb glucose in response to two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group).
27651751	9	28	theme	glucose	1548:1554	arg1	absorption					1556:1565	glucose absorption	1548:1565	glucose absorption	1548:1565	Total small intestinal activities for maltase and α-glucoamylase, and capacities for glucose absorption were similar regardless of diet or exercise.
27651751	2	29	theme	dietary	329:335	arg1	inputs					337:342	dietary inputs	329:342	dietary inputs	329:342	Although the intestine can adapt to changes in the amount and composition of dietary inputs, the responses to the combination of endurance exercise and diet are poorly understood.
27651751	10	30	mod	modified	1722:1729	arg3	altering					1756:1763	altering	1756:1763	altering the capacities of the brush border membrane to digest and absorb carbohydrates	1756:1842	CONCLUSIONS These findings indicate the structural responses of the small intestine to a vegan style diet are modified by exercise, but without altering the capacities of the brush border membrane to digest and absorb carbohydrates.
27651751	10	30	mod	modified	1722:1729	arg1	responses					1663:1671	the structural responses	1648:1671	the structural responses of the small intestine to a vegan style diet	1648:1716	CONCLUSIONS These findings indicate the structural responses of the small intestine to a vegan style diet are modified by exercise, but without altering the capacities of the brush border membrane to digest and absorb carbohydrates.
27651751	10	30	mod	modified	1722:1729	arg3	exercise					1734:1741	exercise	1734:1741	exercise	1734:1741	CONCLUSIONS These findings indicate the structural responses of the small intestine to a vegan style diet are modified by exercise, but without altering the capacities of the brush border membrane to digest and absorb carbohydrates.
27651751	2	31	theme	exercise	391:398	arg1	combination					366:376	the combination	362:376	the combination of endurance exercise and diet	362:407	Although the intestine can adapt to changes in the amount and composition of dietary inputs, the responses to the combination of endurance exercise and diet are poorly understood.
27651751	0	32	theme	Intestinal	0:9	arg1	adaptations					11:21	Intestinal adaptations	0:21	Intestinal adaptations to a combination of different diets with and without endurance exercise	0:93	Intestinal adaptations to a combination of different diets with and without endurance exercise.
27651751	3	33	theme	moderate	732:739	arg1	training					761:768	moderate intensity endurance training	732:768	moderate intensity endurance training	732:768	METHODS We evaluated small intestinal dimensions and mucosal architecture and calculated the capacities of the entire small intestine to digest maltose and maltodextrin and absorb glucose in response to two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group).
27651751	3	33	theme	moderate	732:739	arg1	types					654:658	two different diet types	635:658	two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group)	635:844	METHODS We evaluated small intestinal dimensions and mucosal architecture and calculated the capacities of the entire small intestine to digest maltose and maltodextrin and absorb glucose in response to two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group).
27651751	7	34	theme	increased	1290:1298	arg1	demands					1310:1316	increased energetic demands	1290:1316	increased energetic demands	1290:1316	Despite increased energetic demands, the exercised rats had shorter and lighter intestines with shorter villi (P < 0.05).
27651751	2	35	theme	endurance	381:389	arg1	exercise					391:398	endurance exercise	381:398	endurance exercise	381:398	Although the intestine can adapt to changes in the amount and composition of dietary inputs, the responses to the combination of endurance exercise and diet are poorly understood.
27651751	3	36	theme	intensity	741:749	arg1	training					761:768	moderate intensity endurance training	732:768	moderate intensity endurance training	732:768	METHODS We evaluated small intestinal dimensions and mucosal architecture and calculated the capacities of the entire small intestine to digest maltose and maltodextrin and absorb glucose in response to two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group).
27651751	3	36	theme	intensity	741:749	arg1	types					654:658	two different diet types	635:658	two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group)	635:844	METHODS We evaluated small intestinal dimensions and mucosal architecture and calculated the capacities of the entire small intestine to digest maltose and maltodextrin and absorb glucose in response to two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group).
27651751	9	37	theme	small	1469:1473	arg1	activities					1486:1495	Total small intestinal activities	1463:1495	Total small intestinal activities for maltase and α-glucoamylase	1463:1526	Total small intestinal activities for maltase and α-glucoamylase, and capacities for glucose absorption were similar regardless of diet or exercise.
27651751	1	38	theme	BACKGROUND	96:105	arg1	athletes					117:124	BACKGROUND Endurance athletes	96:124	BACKGROUND Endurance athletes	96:124	BACKGROUND Endurance athletes search for diet regimens that will improve performance and decrease gastrointestinal disturbances during training and events.
27651751	5	39	theme	western	971:977	arg1	diet					979:982	the western diet	967:982	the western diet	967:982	RESULTS Rats fed the western diet gained more weight (P < 0.05) due to more fat mass (P < 0.05), with a similar response for the sedentary compared with the exercised rats in each diet group (P < 0.05).
27651751	10	40	theme	border	1793:1798	arg1	membrane					1800:1807	the brush border membrane	1783:1807	the brush border membrane	1783:1807	CONCLUSIONS These findings indicate the structural responses of the small intestine to a vegan style diet are modified by exercise, but without altering the capacities of the brush border membrane to digest and absorb carbohydrates.
27651751	5	41	dep	RESULTS	950:956	arg1	gained					984:989	gained	984:989	gained	984:989	RESULTS Rats fed the western diet gained more weight (P < 0.05) due to more fat mass (P < 0.05), with a similar response for the sedentary compared with the exercised rats in each diet group (P < 0.05).
27651751	7	42	theme	energetic	1300:1308	arg1	demands					1310:1316	increased energetic demands	1290:1316	increased energetic demands	1290:1316	Despite increased energetic demands, the exercised rats had shorter and lighter intestines with shorter villi (P < 0.05).
27651751	5	43	theme	due	1014:1016	arg1	P < 0.05					1004:1011	P < 0.05	1004:1011	P < 0.05	1004:1011	RESULTS Rats fed the western diet gained more weight (P < 0.05) due to more fat mass (P < 0.05), with a similar response for the sedentary compared with the exercised rats in each diet group (P < 0.05).
27651751	5	43	theme	due	1014:1016	arg1	weight					996:1001	more weight	991:1001	more weight (P < 0.05) due to more fat mass (P < 0.05)	991:1044	RESULTS Rats fed the western diet gained more weight (P < 0.05) due to more fat mass (P < 0.05), with a similar response for the sedentary compared with the exercised rats in each diet group (P < 0.05).
27651751	1	44	theme	Endurance	107:115	arg1	athletes					117:124	BACKGROUND Endurance athletes	96:124	BACKGROUND Endurance athletes	96:124	BACKGROUND Endurance athletes search for diet regimens that will improve performance and decrease gastrointestinal disturbances during training and events.
27651751	3	45	theme	mucosal	485:491	arg1	architecture					493:504	mucosal architecture	485:504	mucosal architecture	485:504	METHODS We evaluated small intestinal dimensions and mucosal architecture and calculated the capacities of the entire small intestine to digest maltose and maltodextrin and absorb glucose in response to two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group).
27651751	5	46	theme	exercised	1107:1115	arg1	rats					1117:1120	the exercised rats	1103:1120	the exercised rats	1103:1120	RESULTS Rats fed the western diet gained more weight (P < 0.05) due to more fat mass (P < 0.05), with a similar response for the sedentary compared with the exercised rats in each diet group (P < 0.05).
27651751	3	47	theme	western	663:669	arg1	diet					677:680	a western human diet	661:680	a western human diet	661:680	METHODS We evaluated small intestinal dimensions and mucosal architecture and calculated the capacities of the entire small intestine to digest maltose and maltodextrin and absorb glucose in response to two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group).
27651751	3	47	theme	western	663:669	arg1	types					654:658	two different diet types	635:658	two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group)	635:844	METHODS We evaluated small intestinal dimensions and mucosal architecture and calculated the capacities of the entire small intestine to digest maltose and maltodextrin and absorb glucose in response to two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group).
27651751	3	48	dep	METHODS	432:438	arg1	evaluated					443:451	evaluated	443:451	evaluated small intestinal dimensions and mucosal architecture	443:504	METHODS We evaluated small intestinal dimensions and mucosal architecture and calculated the capacities of the entire small intestine to digest maltose and maltodextrin and absorb glucose in response to two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group).
27651751	3	48	dep	METHODS	432:438	arg1	calculated					510:519	calculated	510:519	calculated the capacities of the entire small intestine to digest maltose and maltodextrin and absorb glucose in response to two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group)	510:844	METHODS We evaluated small intestinal dimensions and mucosal architecture and calculated the capacities of the entire small intestine to digest maltose and maltodextrin and absorb glucose in response to two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group).
27651751	1	49	theme	gastrointestinal	194:209	arg1	disturbances					211:222	gastrointestinal disturbances	194:222	gastrointestinal disturbances during training and events	194:249	BACKGROUND Endurance athletes search for diet regimens that will improve performance and decrease gastrointestinal disturbances during training and events.
27651751	3	50	theme	intestinal	459:468	arg1	dimensions					470:479	small intestinal dimensions	453:479	small intestinal dimensions	453:479	METHODS We evaluated small intestinal dimensions and mucosal architecture and calculated the capacities of the entire small intestine to digest maltose and maltodextrin and absorb glucose in response to two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group).
27651751	10	51	theme	brush	1787:1791	arg1	membrane					1800:1807	the brush border membrane	1783:1807	the brush border membrane	1783:1807	CONCLUSIONS These findings indicate the structural responses of the small intestine to a vegan style diet are modified by exercise, but without altering the capacities of the brush border membrane to digest and absorb carbohydrates.
27651751	5	52	theme	more	1021:1024	arg1	P < 0.05					1036:1043	P < 0.05	1036:1043	P < 0.05	1036:1043	RESULTS Rats fed the western diet gained more weight (P < 0.05) due to more fat mass (P < 0.05), with a similar response for the sedentary compared with the exercised rats in each diet group (P < 0.05).
27651751	5	52	theme	more	1021:1024	arg1	mass					1030:1033	more fat mass	1021:1033	more fat mass (P < 0.05)	1021:1044	RESULTS Rats fed the western diet gained more weight (P < 0.05) due to more fat mass (P < 0.05), with a similar response for the sedentary compared with the exercised rats in each diet group (P < 0.05).
27651751	10	53	theme	intestine	1686:1694	arg1	responses					1663:1671	the structural responses	1648:1671	the structural responses of the small intestine to a vegan style diet	1648:1716	CONCLUSIONS These findings indicate the structural responses of the small intestine to a vegan style diet are modified by exercise, but without altering the capacities of the brush border membrane to digest and absorb carbohydrates.
27651751	0	54	theme	diets	53:57	arg1	combination					28:38	a combination	26:38	a combination of different diets with and without endurance exercise	26:93	Intestinal adaptations to a combination of different diets with and without endurance exercise.
27651751	10	55	theme	style	1707:1711	arg1	diet					1713:1716	a vegan style diet	1699:1716	a vegan style diet	1699:1716	CONCLUSIONS These findings indicate the structural responses of the small intestine to a vegan style diet are modified by exercise, but without altering the capacities of the brush border membrane to digest and absorb carbohydrates.
27651751	6	56	theme	deeper	1213:1218	arg1	crypts					1220:1225	deeper crypts	1213:1225	deeper crypts with villi that were wider (P < 0.05), but not taller	1213:1279	The Daniel Fast rats had longer and heavier intestines with deeper crypts with villi that were wider (P < 0.05), but not taller.
27651751	5	57	theme	fat	1026:1028	arg1	P < 0.05					1036:1043	P < 0.05	1036:1043	P < 0.05	1036:1043	RESULTS Rats fed the western diet gained more weight (P < 0.05) due to more fat mass (P < 0.05), with a similar response for the sedentary compared with the exercised rats in each diet group (P < 0.05).
27651751	5	57	theme	fat	1026:1028	arg1	mass					1030:1033	more fat mass	1021:1033	more fat mass (P < 0.05)	1021:1044	RESULTS Rats fed the western diet gained more weight (P < 0.05) due to more fat mass (P < 0.05), with a similar response for the sedentary compared with the exercised rats in each diet group (P < 0.05).
27651751	3	58	theme	absorb	605:610	arg1	glucose					612:618	absorb glucose	605:618	absorb glucose	605:618	METHODS We evaluated small intestinal dimensions and mucosal architecture and calculated the capacities of the entire small intestine to digest maltose and maltodextrin and absorb glucose in response to two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group).
27651751	7	59	theme	exercised	1323:1331	arg1	rats					1333:1336	the exercised rats	1319:1336	the exercised rats	1319:1336	Despite increased energetic demands, the exercised rats had shorter and lighter intestines with shorter villi (P < 0.05).
27651751	0	60	theme	different	43:51	arg1	diets					53:57	different diets	43:57	different diets	43:57	Intestinal adaptations to a combination of different diets with and without endurance exercise.
27651751	3	61	theme	endurance	751:759	arg1	training					761:768	moderate intensity endurance training	732:768	moderate intensity endurance training	732:768	METHODS We evaluated small intestinal dimensions and mucosal architecture and calculated the capacities of the entire small intestine to digest maltose and maltodextrin and absorb glucose in response to two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group).
27651751	3	61	theme	endurance	751:759	arg1	types					654:658	two different diet types	635:658	two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group)	635:844	METHODS We evaluated small intestinal dimensions and mucosal architecture and calculated the capacities of the entire small intestine to digest maltose and maltodextrin and absorb glucose in response to two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group).
27651751	4	62	theme	diet	865:868	arg1	influences					851:860	The influences	847:860	The influences	847:860	The influences of diet and exercise, alone and in combination, were analyzed by analysis of variation.
27651751	5	63	theme	more	991:994	arg1	P < 0.05					1004:1011	P < 0.05	1004:1011	P < 0.05	1004:1011	RESULTS Rats fed the western diet gained more weight (P < 0.05) due to more fat mass (P < 0.05), with a similar response for the sedentary compared with the exercised rats in each diet group (P < 0.05).
27651751	5	63	theme	more	991:994	arg1	weight					996:1001	more weight	991:1001	more weight (P < 0.05) due to more fat mass (P < 0.05)	991:1044	RESULTS Rats fed the western diet gained more weight (P < 0.05) due to more fat mass (P < 0.05), with a similar response for the sedentary compared with the exercised rats in each diet group (P < 0.05).
27651751	10	64	dep	digest	1812:1817	arg1	carbohydrates					1830:1842	carbohydrates	1830:1842	carbohydrates	1830:1842	CONCLUSIONS These findings indicate the structural responses of the small intestine to a vegan style diet are modified by exercise, but without altering the capacities of the brush border membrane to digest and absorb carbohydrates.
27651751	6	65	theme	longer	1178:1183	arg1	intestines					1197:1206	longer and heavier intestines	1178:1206	longer and heavier intestines	1178:1206	The Daniel Fast rats had longer and heavier intestines with deeper crypts with villi that were wider (P < 0.05), but not taller.
27651751	7	66	theme	shorter	1378:1384	arg1	P < 0.05					1393:1400	P < 0.05	1393:1400	P < 0.05	1393:1400	Despite increased energetic demands, the exercised rats had shorter and lighter intestines with shorter villi (P < 0.05).
27651751	7	66	theme	shorter	1378:1384	arg1	villi					1386:1390	shorter villi	1378:1390	shorter villi (P < 0.05)	1378:1401	Despite increased energetic demands, the exercised rats had shorter and lighter intestines with shorter villi (P < 0.05).
27651751	3	67	theme	different	639:647	arg1	lifestyle					797:805	a no-exercise sedentary lifestyle	773:805	a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group)	773:844	METHODS We evaluated small intestinal dimensions and mucosal architecture and calculated the capacities of the entire small intestine to digest maltose and maltodextrin and absorb glucose in response to two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group).
27651751	3	67	theme	different	639:647	arg1	types					654:658	two different diet types	635:658	two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group)	635:844	METHODS We evaluated small intestinal dimensions and mucosal architecture and calculated the capacities of the entire small intestine to digest maltose and maltodextrin and absorb glucose in response to two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group).
27651751	3	67	theme	different	639:647	arg1	diet					717:720	a vegan style diet	703:720	a vegan style diet	703:720	METHODS We evaluated small intestinal dimensions and mucosal architecture and calculated the capacities of the entire small intestine to digest maltose and maltodextrin and absorb glucose in response to two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group).
27651751	3	67	theme	different	639:647	arg1	training					761:768	moderate intensity endurance training	732:768	moderate intensity endurance training	732:768	METHODS We evaluated small intestinal dimensions and mucosal architecture and calculated the capacities of the entire small intestine to digest maltose and maltodextrin and absorb glucose in response to two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group).
27651751	3	67	theme	different	639:647	arg1	Daniel					690:695	Daniel	690:695	Daniel	690:695	METHODS We evaluated small intestinal dimensions and mucosal architecture and calculated the capacities of the entire small intestine to digest maltose and maltodextrin and absorb glucose in response to two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group).
27651751	3	67	theme	different	639:647	arg1	diet					677:680	a western human diet	661:680	a western human diet	661:680	METHODS We evaluated small intestinal dimensions and mucosal architecture and calculated the capacities of the entire small intestine to digest maltose and maltodextrin and absorb glucose in response to two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group).
27651751	7	68	theme	lighter	1354:1360	arg1	intestines					1362:1371	shorter and lighter intestines	1342:1371	shorter and lighter intestines	1342:1371	Despite increased energetic demands, the exercised rats had shorter and lighter intestines with shorter villi (P < 0.05).
27651751	2	69	from	changes	288:294	arg1	composition					314:324	composition	314:324	composition	314:324	Although the intestine can adapt to changes in the amount and composition of dietary inputs, the responses to the combination of endurance exercise and diet are poorly understood.
27651751	2	69	from	changes	288:294	arg1	inputs					337:342	dietary inputs	329:342	dietary inputs	329:342	Although the intestine can adapt to changes in the amount and composition of dietary inputs, the responses to the combination of endurance exercise and diet are poorly understood.
27651751	2	69	from	changes	288:294	arg1	amount					303:308	amount	303:308	amount	303:308	Although the intestine can adapt to changes in the amount and composition of dietary inputs, the responses to the combination of endurance exercise and diet are poorly understood.
27651751	3	70	dep	types	654:658	arg1	lifestyle					797:805	a no-exercise sedentary lifestyle	773:805	a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group)	773:844	METHODS We evaluated small intestinal dimensions and mucosal architecture and calculated the capacities of the entire small intestine to digest maltose and maltodextrin and absorb glucose in response to two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group).
27651751	3	70	dep	types	654:658	arg1	types					654:658	two different diet types	635:658	two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group)	635:844	METHODS We evaluated small intestinal dimensions and mucosal architecture and calculated the capacities of the entire small intestine to digest maltose and maltodextrin and absorb glucose in response to two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group).
27651751	3	70	dep	types	654:658	arg1	response					623:630	response	623:630	response	623:630	METHODS We evaluated small intestinal dimensions and mucosal architecture and calculated the capacities of the entire small intestine to digest maltose and maltodextrin and absorb glucose in response to two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group).
27651751	3	70	dep	types	654:658	arg1	diet					717:720	a vegan style diet	703:720	a vegan style diet	703:720	METHODS We evaluated small intestinal dimensions and mucosal architecture and calculated the capacities of the entire small intestine to digest maltose and maltodextrin and absorb glucose in response to two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group).
27651751	3	70	dep	types	654:658	arg1	training					761:768	moderate intensity endurance training	732:768	moderate intensity endurance training	732:768	METHODS We evaluated small intestinal dimensions and mucosal architecture and calculated the capacities of the entire small intestine to digest maltose and maltodextrin and absorb glucose in response to two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group).
27651751	3	70	dep	types	654:658	arg1	Daniel					690:695	Daniel	690:695	Daniel	690:695	METHODS We evaluated small intestinal dimensions and mucosal architecture and calculated the capacities of the entire small intestine to digest maltose and maltodextrin and absorb glucose in response to two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group).
27651751	3	70	dep	types	654:658	arg1	diet					677:680	a western human diet	661:680	a western human diet	661:680	METHODS We evaluated small intestinal dimensions and mucosal architecture and calculated the capacities of the entire small intestine to digest maltose and maltodextrin and absorb glucose in response to two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group).
27651751	4	71	theme	exercise	874:881	arg1	influences					851:860	The influences	847:860	The influences	847:860	The influences of diet and exercise, alone and in combination, were analyzed by analysis of variation.
27651751	3	72	theme	human	671:675	arg1	diet					677:680	a western human diet	661:680	a western human diet	661:680	METHODS We evaluated small intestinal dimensions and mucosal architecture and calculated the capacities of the entire small intestine to digest maltose and maltodextrin and absorb glucose in response to two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group).
27651751	3	72	theme	human	671:675	arg1	types					654:658	two different diet types	635:658	two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group)	635:844	METHODS We evaluated small intestinal dimensions and mucosal architecture and calculated the capacities of the entire small intestine to digest maltose and maltodextrin and absorb glucose in response to two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group).
27651751	3	73	theme	diet	649:652	arg1	lifestyle					797:805	a no-exercise sedentary lifestyle	773:805	a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group)	773:844	METHODS We evaluated small intestinal dimensions and mucosal architecture and calculated the capacities of the entire small intestine to digest maltose and maltodextrin and absorb glucose in response to two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group).
27651751	3	73	theme	diet	649:652	arg1	types					654:658	two different diet types	635:658	two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group)	635:844	METHODS We evaluated small intestinal dimensions and mucosal architecture and calculated the capacities of the entire small intestine to digest maltose and maltodextrin and absorb glucose in response to two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group).
27651751	3	73	theme	diet	649:652	arg1	diet					717:720	a vegan style diet	703:720	a vegan style diet	703:720	METHODS We evaluated small intestinal dimensions and mucosal architecture and calculated the capacities of the entire small intestine to digest maltose and maltodextrin and absorb glucose in response to two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group).
27651751	3	73	theme	diet	649:652	arg1	training					761:768	moderate intensity endurance training	732:768	moderate intensity endurance training	732:768	METHODS We evaluated small intestinal dimensions and mucosal architecture and calculated the capacities of the entire small intestine to digest maltose and maltodextrin and absorb glucose in response to two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group).
27651751	3	73	theme	diet	649:652	arg1	Daniel					690:695	Daniel	690:695	Daniel	690:695	METHODS We evaluated small intestinal dimensions and mucosal architecture and calculated the capacities of the entire small intestine to digest maltose and maltodextrin and absorb glucose in response to two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group).
27651751	3	73	theme	diet	649:652	arg1	diet					677:680	a western human diet	661:680	a western human diet	661:680	METHODS We evaluated small intestinal dimensions and mucosal architecture and calculated the capacities of the entire small intestine to digest maltose and maltodextrin and absorb glucose in response to two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group).
27651751	2	74	theme	inputs	337:342	arg1	composition					314:324	composition	314:324	composition	314:324	Although the intestine can adapt to changes in the amount and composition of dietary inputs, the responses to the combination of endurance exercise and diet are poorly understood.
27651751	2	74	theme	inputs	337:342	arg1	inputs					337:342	dietary inputs	329:342	dietary inputs	329:342	Although the intestine can adapt to changes in the amount and composition of dietary inputs, the responses to the combination of endurance exercise and diet are poorly understood.
27651751	2	74	theme	inputs	337:342	arg1	amount					303:308	amount	303:308	amount	303:308	Although the intestine can adapt to changes in the amount and composition of dietary inputs, the responses to the combination of endurance exercise and diet are poorly understood.
27651751	10	75	theme	structural	1652:1661	arg1	responses					1663:1671	the structural responses	1648:1671	the structural responses of the small intestine to a vegan style diet	1648:1716	CONCLUSIONS These findings indicate the structural responses of the small intestine to a vegan style diet are modified by exercise, but without altering the capacities of the brush border membrane to digest and absorb carbohydrates.
27651751	10	76	theme	vegan	1701:1705	arg1	diet					1713:1716	a vegan style diet	1699:1716	a vegan style diet	1699:1716	CONCLUSIONS These findings indicate the structural responses of the small intestine to a vegan style diet are modified by exercise, but without altering the capacities of the brush border membrane to digest and absorb carbohydrates.
27651751	3	77	theme	no-exercise	775:785	arg1	lifestyle					797:805	a no-exercise sedentary lifestyle	773:805	a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group)	773:844	METHODS We evaluated small intestinal dimensions and mucosal architecture and calculated the capacities of the entire small intestine to digest maltose and maltodextrin and absorb glucose in response to two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group).
27651751	3	77	theme	no-exercise	775:785	arg1	types					654:658	two different diet types	635:658	two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group)	635:844	METHODS We evaluated small intestinal dimensions and mucosal architecture and calculated the capacities of the entire small intestine to digest maltose and maltodextrin and absorb glucose in response to two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group).
27651751	3	78	theme	entire	543:548	arg1	intestine					556:564	the entire small intestine	539:564	the entire small intestine	539:564	METHODS We evaluated small intestinal dimensions and mucosal architecture and calculated the capacities of the entire small intestine to digest maltose and maltodextrin and absorb glucose in response to two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group).
27651751	3	79	theme	sedentary	787:795	arg1	lifestyle					797:805	a no-exercise sedentary lifestyle	773:805	a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group)	773:844	METHODS We evaluated small intestinal dimensions and mucosal architecture and calculated the capacities of the entire small intestine to digest maltose and maltodextrin and absorb glucose in response to two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group).
27651751	3	79	theme	sedentary	787:795	arg1	types					654:658	two different diet types	635:658	two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group)	635:844	METHODS We evaluated small intestinal dimensions and mucosal architecture and calculated the capacities of the entire small intestine to digest maltose and maltodextrin and absorb glucose in response to two different diet types; a western human diet and the Daniel Fast, a vegan style diet, and with moderate intensity endurance training or a no-exercise sedentary lifestyle for a 13 week period (n = 7 per group).
28698456	0	0	theme	Hep-arin	84:91	arg1	Features					66:73	Structural Features	55:73	Structural Features of Crude Hep-arin	55:91	Combining NMR Spectroscopy and Chemometrics to Monitor Structural Features of Crude Hep-arin.
28698456	3	1	theme	crude	650:654	arg1	structure					664:672	crude heparin structure	650:672	crude heparin structure	650:672	Although a number of analytical techniques have been applied to characterize heparin APIs, few of them have been applied for crude heparin structure and composition analyses.
28698456	1	2	theme	heparin	145:151	arg1	chain					160:164	the heparin supply chain	141:164	the heparin supply chain	141:164	Because of the complexity and global nature of the heparin supply chain, the control of heparin quality during manufacturing steps is essential to ensure the safety of the final active pharmaceutical ingredient (API).
28698456	0	3	theme	Crude	78:82	arg1	Hep-arin					84:91	Crude Hep-arin	78:91	Crude Hep-arin	78:91	Combining NMR Spectroscopy and Chemometrics to Monitor Structural Features of Crude Hep-arin.
28698456	3	4	dep	structure	664:672	arg1	analyses					690:697	analyses	690:697	analyses	690:697	Although a number of analytical techniques have been applied to characterize heparin APIs, few of them have been applied for crude heparin structure and composition analyses.
28698456	1	5	theme	supply	153:158	arg1	chain					160:164	the heparin supply chain	141:164	the heparin supply chain	141:164	Because of the complexity and global nature of the heparin supply chain, the control of heparin quality during manufacturing steps is essential to ensure the safety of the final active pharmaceutical ingredient (API).
28698456	4	6	theme	crude	795:799	arg1	samples					809:815	88 crude heparin samples	792:815	88 crude heparin samples	792:815	Here, to address this issue, NMR spectroscopy and chemometrics were applied to characterize 88 crude heparin samples.
28698456	7	7	theme	orthogonal	1088:1097	arg1	approaches					1099:1108	These orthogonal approaches	1082:1108	These orthogonal approaches	1082:1108	These orthogonal approaches differentiated samples according their glycosaminoglycan (GAG) composition and their mono and disaccharide composition and structure for each GAG family (e.g., heparin/heparan, dermatan sulfate, and chondroitin sulfate A).
28698456	8	8	used	used	1398:1401	arg2	analysis					1378:1385	multivariate analysis	1365:1385	multivariate analysis (PCA)	1365:1391	Moreover, quantitative HSQC and multivariate analysis (PCA) were used to distinguish between crude heparin of different animal and tissue sources.
28698456	8	8	used	used	1398:1401	arg2	HSQC					1356:1359	quantitative HSQC	1343:1359	quantitative HSQC	1343:1359	Moreover, quantitative HSQC and multivariate analysis (PCA) were used to distinguish between crude heparin of different animal and tissue sources.
28698456	8	8	used	used	1398:1401	arg2	PCA					1388:1390	PCA	1388:1390	PCA	1388:1390	Moreover, quantitative HSQC and multivariate analysis (PCA) were used to distinguish between crude heparin of different animal and tissue sources.
28698456	2	9	theme	crude	458:462	arg1	heparin					464:470	the crude heparin before it is purified to API under cGMP conditions	454:521	the crude heparin before it is purified to API under cGMP conditions	454:521	For this reason, there is a need to develop consistent analytical methods able to assess the quality of heparin early in production (i.e., as the crude heparin before it is purified to API under cGMP conditions).
28698456	2	10	dep	assess	394:399	arg1	i.e.					445:448	i.e.	445:448	i.e.	445:448	For this reason, there is a need to develop consistent analytical methods able to assess the quality of heparin early in production (i.e., as the crude heparin before it is purified to API under cGMP conditions).
28698456	7	11	theme	disaccharide	1204:1215	arg1	composition					1217:1227	their mono and disaccharide composition	1189:1227	composition	1217:1227	These orthogonal approaches differentiated samples according their glycosaminoglycan (GAG) composition and their mono and disaccharide composition and structure for each GAG family (e.g., heparin/heparan, dermatan sulfate, and chondroitin sulfate A).
28698456	7	12	theme	chondroitin	1309:1319	arg1	sulfate					1321:1327	chondroitin sulfate	1309:1327	chondroitin sulfate	1309:1327	These orthogonal approaches differentiated samples according their glycosaminoglycan (GAG) composition and their mono and disaccharide composition and structure for each GAG family (e.g., heparin/heparan, dermatan sulfate, and chondroitin sulfate A).
28698456	1	13	theme	chain	160:164	arg1	complexity					109:118	the complexity	105:118	the complexity	105:118	Because of the complexity and global nature of the heparin supply chain, the control of heparin quality during manufacturing steps is essential to ensure the safety of the final active pharmaceutical ingredient (API).
28698456	1	13	theme	chain	160:164	arg1	nature					131:136	global nature	124:136	global nature	124:136	Because of the complexity and global nature of the heparin supply chain, the control of heparin quality during manufacturing steps is essential to ensure the safety of the final active pharmaceutical ingredient (API).
28698456	6	14	theme	HPLC	969:972	arg1	data					974:977	The HPLC data	965:977	The HPLC data	965:977	The HPLC data showed that the chemometric analysis of the NMR data differentiated the samples based on their purity.
28698456	0	15	theme	NMR	10:12	arg1	Spectroscopy					14:25	NMR Spectroscopy	10:25	NMR Spectroscopy	10:25	Combining NMR Spectroscopy and Chemometrics to Monitor Structural Features of Crude Hep-arin.
28698456	3	16	theme	heparin	656:662	arg1	structure					664:672	crude heparin structure	650:672	crude heparin structure	650:672	Although a number of analytical techniques have been applied to characterize heparin APIs, few of them have been applied for crude heparin structure and composition analyses.
28698456	7	17	dep	heparin/heparan	1270:1284	arg1	e.g.					1264:1267	e.g.	1264:1267	e.g.	1264:1267	These orthogonal approaches differentiated samples according their glycosaminoglycan (GAG) composition and their mono and disaccharide composition and structure for each GAG family (e.g., heparin/heparan, dermatan sulfate, and chondroitin sulfate A).
28698456	5	18	theme	strong	852:857	arg1	SAX-HPLC					880:887	SAX-HPLC	880:887	SAX-HPLC	880:887	The samples were also analyzed by strong anion exchange HPLC (SAX-HPLC) as an orthogonal check of the purity levels of the crudes analyzed by NMR.
28698456	5	18	theme	strong	852:857	arg1	HPLC					874:877	strong anion exchange HPLC	852:877	strong anion exchange HPLC (SAX-HPLC)	852:888	The samples were also analyzed by strong anion exchange HPLC (SAX-HPLC) as an orthogonal check of the purity levels of the crudes analyzed by NMR.
28698456	8	19	theme	crude	1426:1430	arg1	heparin					1432:1438	crude heparin	1426:1438	crude heparin of different animal and tissue sources	1426:1477	Moreover, quantitative HSQC and multivariate analysis (PCA) were used to distinguish between crude heparin of different animal and tissue sources.
28698456	5	20	theme	levels	927:932	arg1	check					907:911	an orthogonal check	893:911	an orthogonal check of the purity levels of the crudes analyzed by NMR	893:962	The samples were also analyzed by strong anion exchange HPLC (SAX-HPLC) as an orthogonal check of the purity levels of the crudes analyzed by NMR.
28698456	5	20	theme	levels	927:932	arg1	samples					822:828	The samples	818:828	The samples	818:828	The samples were also analyzed by strong anion exchange HPLC (SAX-HPLC) as an orthogonal check of the purity levels of the crudes analyzed by NMR.
28698456	5	21	theme	anion	859:863	arg1	SAX-HPLC					880:887	SAX-HPLC	880:887	SAX-HPLC	880:887	The samples were also analyzed by strong anion exchange HPLC (SAX-HPLC) as an orthogonal check of the purity levels of the crudes analyzed by NMR.
28698456	5	21	theme	anion	859:863	arg1	HPLC					874:877	strong anion exchange HPLC	852:877	strong anion exchange HPLC (SAX-HPLC)	852:888	The samples were also analyzed by strong anion exchange HPLC (SAX-HPLC) as an orthogonal check of the purity levels of the crudes analyzed by NMR.
28698456	2	22	theme	able	386:389	arg1	methods					378:384	consistent analytical methods	356:384	consistent analytical methods able to assess the quality of heparin early in production (i.e., as the crude heparin before it is purified to API under cGMP conditions)	356:522	For this reason, there is a need to develop consistent analytical methods able to assess the quality of heparin early in production (i.e., as the crude heparin before it is purified to API under cGMP conditions).
28698456	6	23	theme	data	1027:1030	arg1	analysis					1007:1014	the chemometric analysis	991:1014	the chemometric analysis of the NMR data	991:1030	The HPLC data showed that the chemometric analysis of the NMR data differentiated the samples based on their purity.
28698456	1	24	theme	final	266:270	arg1	ingredient					294:303	the final active pharmaceutical ingredient	262:303	the final active pharmaceutical ingredient (API)	262:309	Because of the complexity and global nature of the heparin supply chain, the control of heparin quality during manufacturing steps is essential to ensure the safety of the final active pharmaceutical ingredient (API).
28698456	1	24	theme	final	266:270	arg1	API					306:308	API	306:308	API	306:308	Because of the complexity and global nature of the heparin supply chain, the control of heparin quality during manufacturing steps is essential to ensure the safety of the final active pharmaceutical ingredient (API).
28698456	5	25	theme	exchange	865:872	arg1	SAX-HPLC					880:887	SAX-HPLC	880:887	SAX-HPLC	880:887	The samples were also analyzed by strong anion exchange HPLC (SAX-HPLC) as an orthogonal check of the purity levels of the crudes analyzed by NMR.
28698456	5	25	theme	exchange	865:872	arg1	HPLC					874:877	strong anion exchange HPLC	852:877	strong anion exchange HPLC (SAX-HPLC)	852:888	The samples were also analyzed by strong anion exchange HPLC (SAX-HPLC) as an orthogonal check of the purity levels of the crudes analyzed by NMR.
28698456	8	26	theme	different	1443:1451	arg1	animal					1453:1458	different animal and tissue sources	1443:1477	animal	1453:1458	Moreover, quantitative HSQC and multivariate analysis (PCA) were used to distinguish between crude heparin of different animal and tissue sources.
28698456	1	27	theme	heparin	182:188	arg1	quality					190:196	heparin quality	182:196	heparin quality	182:196	Because of the complexity and global nature of the heparin supply chain, the control of heparin quality during manufacturing steps is essential to ensure the safety of the final active pharmaceutical ingredient (API).
28698456	1	28	theme	active	272:277	arg1	ingredient					294:303	the final active pharmaceutical ingredient	262:303	the final active pharmaceutical ingredient (API)	262:309	Because of the complexity and global nature of the heparin supply chain, the control of heparin quality during manufacturing steps is essential to ensure the safety of the final active pharmaceutical ingredient (API).
28698456	1	28	theme	active	272:277	arg1	API					306:308	API	306:308	API	306:308	Because of the complexity and global nature of the heparin supply chain, the control of heparin quality during manufacturing steps is essential to ensure the safety of the final active pharmaceutical ingredient (API).
28698456	1	29	theme	quality	190:196	arg1	control					171:177	the control	167:177	the control of heparin quality during manufacturing steps	167:223	Because of the complexity and global nature of the heparin supply chain, the control of heparin quality during manufacturing steps is essential to ensure the safety of the final active pharmaceutical ingredient (API).
28698456	1	29	theme	quality	190:196	arg1	essential					228:236	essential	228:236	essential	228:236	Because of the complexity and global nature of the heparin supply chain, the control of heparin quality during manufacturing steps is essential to ensure the safety of the final active pharmaceutical ingredient (API).
28698456	2	30	theme	analytical	367:376	arg1	methods					378:384	consistent analytical methods	356:384	consistent analytical methods able to assess the quality of heparin early in production (i.e., as the crude heparin before it is purified to API under cGMP conditions)	356:522	For this reason, there is a need to develop consistent analytical methods able to assess the quality of heparin early in production (i.e., as the crude heparin before it is purified to API under cGMP conditions).
28698456	1	31	theme	pharmaceutical	279:292	arg1	ingredient					294:303	the final active pharmaceutical ingredient	262:303	the final active pharmaceutical ingredient (API)	262:309	Because of the complexity and global nature of the heparin supply chain, the control of heparin quality during manufacturing steps is essential to ensure the safety of the final active pharmaceutical ingredient (API).
28698456	1	31	theme	pharmaceutical	279:292	arg1	API					306:308	API	306:308	API	306:308	Because of the complexity and global nature of the heparin supply chain, the control of heparin quality during manufacturing steps is essential to ensure the safety of the final active pharmaceutical ingredient (API).
28698456	7	32	theme	dermatan	1287:1294	arg1	sulfate					1296:1302	dermatan sulfate	1287:1302	dermatan sulfate	1287:1302	These orthogonal approaches differentiated samples according their glycosaminoglycan (GAG) composition and their mono and disaccharide composition and structure for each GAG family (e.g., heparin/heparan, dermatan sulfate, and chondroitin sulfate A).
28698456	3	33	theme	techniques	557:566	arg1	number					536:541	a number	534:541	a number of analytical techniques	534:566	Although a number of analytical techniques have been applied to characterize heparin APIs, few of them have been applied for crude heparin structure and composition analyses.
28698456	2	34	theme	consistent	356:365	arg1	methods					378:384	consistent analytical methods	356:384	consistent analytical methods able to assess the quality of heparin early in production (i.e., as the crude heparin before it is purified to API under cGMP conditions)	356:522	For this reason, there is a need to develop consistent analytical methods able to assess the quality of heparin early in production (i.e., as the crude heparin before it is purified to API under cGMP conditions).
28698456	0	35	theme	Structural	55:64	arg1	Features					66:73	Structural Features	55:73	Structural Features of Crude Hep-arin	55:91	Combining NMR Spectroscopy and Chemometrics to Monitor Structural Features of Crude Hep-arin.
28698456	1	36	theme	ingredient	294:303	arg1	safety					252:257	the safety	248:257	the safety of the final active pharmaceutical ingredient (API)	248:309	Because of the complexity and global nature of the heparin supply chain, the control of heparin quality during manufacturing steps is essential to ensure the safety of the final active pharmaceutical ingredient (API).
28698456	2	37	theme	cGMP	507:510	arg1	conditions					512:521	cGMP conditions	507:521	cGMP conditions	507:521	For this reason, there is a need to develop consistent analytical methods able to assess the quality of heparin early in production (i.e., as the crude heparin before it is purified to API under cGMP conditions).
28698456	5	38	theme	purity	920:925	arg1	levels					927:932	the purity levels	916:932	the purity levels of the crudes analyzed by NMR	916:962	The samples were also analyzed by strong anion exchange HPLC (SAX-HPLC) as an orthogonal check of the purity levels of the crudes analyzed by NMR.
28698456	1	39	theme	complexity	109:118	arg1	essential					228:236	essential	228:236	essential	228:236	Because of the complexity and global nature of the heparin supply chain, the control of heparin quality during manufacturing steps is essential to ensure the safety of the final active pharmaceutical ingredient (API).
28698456	1	39	theme	complexity	109:118	arg1	control					171:177	the control	167:177	the control of heparin quality during manufacturing steps	167:223	Because of the complexity and global nature of the heparin supply chain, the control of heparin quality during manufacturing steps is essential to ensure the safety of the final active pharmaceutical ingredient (API).
28698456	8	40	theme	sources	1471:1477	arg1	heparin					1432:1438	crude heparin	1426:1438	crude heparin of different animal and tissue sources	1426:1477	Moreover, quantitative HSQC and multivariate analysis (PCA) were used to distinguish between crude heparin of different animal and tissue sources.
28698456	4	41	theme	heparin	801:807	arg1	samples					809:815	88 crude heparin samples	792:815	88 crude heparin samples	792:815	Here, to address this issue, NMR spectroscopy and chemometrics were applied to characterize 88 crude heparin samples.
28698456	3	42	theme	heparin	602:608	arg1	APIs					610:613	heparin APIs	602:613	heparin APIs	602:613	Although a number of analytical techniques have been applied to characterize heparin APIs, few of them have been applied for crude heparin structure and composition analyses.
28698456	4	43	theme	NMR	729:731	arg1	spectroscopy					733:744	NMR spectroscopy	729:744	NMR spectroscopy	729:744	Here, to address this issue, NMR spectroscopy and chemometrics were applied to characterize 88 crude heparin samples.
28698456	8	44	theme	quantitative	1343:1354	arg1	HSQC					1356:1359	quantitative HSQC	1343:1359	quantitative HSQC	1343:1359	Moreover, quantitative HSQC and multivariate analysis (PCA) were used to distinguish between crude heparin of different animal and tissue sources.
28698456	8	45	theme	tissue	1464:1469	arg1	sources					1471:1477	different animal and tissue sources	1443:1477	sources	1471:1477	Moreover, quantitative HSQC and multivariate analysis (PCA) were used to distinguish between crude heparin of different animal and tissue sources.
28698456	1	46	theme	global	124:129	arg1	nature					131:136	global nature	124:136	global nature	124:136	Because of the complexity and global nature of the heparin supply chain, the control of heparin quality during manufacturing steps is essential to ensure the safety of the final active pharmaceutical ingredient (API).
28698456	6	47	theme	NMR	1023:1025	arg1	data					1027:1030	the NMR data	1019:1030	the NMR data	1019:1030	The HPLC data showed that the chemometric analysis of the NMR data differentiated the samples based on their purity.
28698456	1	48	theme	manufacturing	205:217	arg1	steps					219:223	manufacturing steps	205:223	manufacturing steps	205:223	Because of the complexity and global nature of the heparin supply chain, the control of heparin quality during manufacturing steps is essential to ensure the safety of the final active pharmaceutical ingredient (API).
28698456	5	49	theme	crudes	941:946	arg1	levels					927:932	the purity levels	916:932	the purity levels of the crudes analyzed by NMR	916:962	The samples were also analyzed by strong anion exchange HPLC (SAX-HPLC) as an orthogonal check of the purity levels of the crudes analyzed by NMR.
28698456	3	50	theme	analytical	546:555	arg1	techniques					557:566	analytical techniques	546:566	analytical techniques	546:566	Although a number of analytical techniques have been applied to characterize heparin APIs, few of them have been applied for crude heparin structure and composition analyses.
28698456	1	51	theme	nature	131:136	arg1	essential					228:236	essential	228:236	essential	228:236	Because of the complexity and global nature of the heparin supply chain, the control of heparin quality during manufacturing steps is essential to ensure the safety of the final active pharmaceutical ingredient (API).
28698456	1	51	theme	nature	131:136	arg1	control					171:177	the control	167:177	the control of heparin quality during manufacturing steps	167:223	Because of the complexity and global nature of the heparin supply chain, the control of heparin quality during manufacturing steps is essential to ensure the safety of the final active pharmaceutical ingredient (API).
28698456	6	52	theme	chemometric	995:1005	arg1	analysis					1007:1014	the chemometric analysis	991:1014	the chemometric analysis of the NMR data	991:1030	The HPLC data showed that the chemometric analysis of the NMR data differentiated the samples based on their purity.
28698456	8	53	theme	multivariate	1365:1376	arg1	PCA					1388:1390	PCA	1388:1390	PCA	1388:1390	Moreover, quantitative HSQC and multivariate analysis (PCA) were used to distinguish between crude heparin of different animal and tissue sources.
28698456	8	53	theme	multivariate	1365:1376	arg1	analysis					1378:1385	multivariate analysis	1365:1385	multivariate analysis (PCA)	1365:1391	Moreover, quantitative HSQC and multivariate analysis (PCA) were used to distinguish between crude heparin of different animal and tissue sources.
28698456	5	54	theme	orthogonal	896:905	arg1	check					907:911	an orthogonal check	893:911	an orthogonal check of the purity levels of the crudes analyzed by NMR	893:962	The samples were also analyzed by strong anion exchange HPLC (SAX-HPLC) as an orthogonal check of the purity levels of the crudes analyzed by NMR.
28698456	5	54	theme	orthogonal	896:905	arg1	samples					822:828	The samples	818:828	The samples	818:828	The samples were also analyzed by strong anion exchange HPLC (SAX-HPLC) as an orthogonal check of the purity levels of the crudes analyzed by NMR.
28698456	7	55	theme	glycosaminoglycan	1149:1165	arg1	composition					1173:1183	their glycosaminoglycan (GAG) composition	1143:1183	their glycosaminoglycan (GAG) composition	1143:1183	These orthogonal approaches differentiated samples according their glycosaminoglycan (GAG) composition and their mono and disaccharide composition and structure for each GAG family (e.g., heparin/heparan, dermatan sulfate, and chondroitin sulfate A).
28698456	2	56	theme	heparin	416:422	arg1	quality					405:411	the quality	401:411	the quality of heparin	401:422	For this reason, there is a need to develop consistent analytical methods able to assess the quality of heparin early in production (i.e., as the crude heparin before it is purified to API under cGMP conditions).
28698456	7	57	theme	GAG	1252:1254	arg1	family					1256:1261	each GAG family	1247:1261	each GAG family	1247:1261	These orthogonal approaches differentiated samples according their glycosaminoglycan (GAG) composition and their mono and disaccharide composition and structure for each GAG family (e.g., heparin/heparan, dermatan sulfate, and chondroitin sulfate A).
28698456	3	58	theme	them	623:626	arg1	few					616:618	few	616:618	few	616:618	Although a number of analytical techniques have been applied to characterize heparin APIs, few of them have been applied for crude heparin structure and composition analyses.
28698456	8	59	theme	animal	1453:1458	arg1	heparin					1432:1438	crude heparin	1426:1438	crude heparin of different animal and tissue sources	1426:1477	Moreover, quantitative HSQC and multivariate analysis (PCA) were used to distinguish between crude heparin of different animal and tissue sources.
27689418	1	0	from	resistance	398:407	arg1	function					350:357	the function	346:357	the function of chemical composition changes in the resistance of the E. faecalis biofilm to an extreme environment	346:460	ABSTACT This study aimed to investigate the dynamic changes that occur in the chemical composition of an Enterococcus faecalis (E. faecalis) biofilm under conditions of starvation and in an alkaline environment and to explore the function of chemical composition changes in the resistance of the E. faecalis biofilm to an extreme environment.
27689418	5	1	from	polysaccharides	1003:1017	arg1	medium					1030:1035	culture medium	1022:1035	culture medium with pH values of 9 and 11	1022:1062	In addition, the percentage of water-soluble polysaccharides in culture medium with pH values of 9 and 11 gradually decreased.
27689418	3	2	theme	chemical	704:711	arg1	composition					713:723	chemical composition	704:723	chemical composition	704:723	During the formation of the biofilm, the pH value and nutritional condition of the culture medium were changed, and the changes in chemical composition were observed using biochemical measures.
27689418	4	3	theme	other	940:944	arg1	conditions					946:955	other conditions	940:955	other conditions	940:955	The results showed that, when the pH value of the culture medium was 11, the percentage of water-insoluble polysaccharides in the biofilm was significantly lower than under other conditions.
27689418	3	4	theme	culture	656:662	arg1	medium					664:669	the culture medium	652:669	the culture medium	652:669	During the formation of the biofilm, the pH value and nutritional condition of the culture medium were changed, and the changes in chemical composition were observed using biochemical measures.
27689418	4	5	theme	culture	817:823	arg1	medium					825:830	the culture medium	813:830	the culture medium	813:830	The results showed that, when the pH value of the culture medium was 11, the percentage of water-insoluble polysaccharides in the biofilm was significantly lower than under other conditions.
27689418	1	6	theme	dynamic	164:170	arg1	changes					172:178	the dynamic changes	160:178	the dynamic changes that occur in the chemical composition of an Enterococcus faecalis (E. faecalis) biofilm under conditions of starvation and in an alkaline environment	160:329	ABSTACT This study aimed to investigate the dynamic changes that occur in the chemical composition of an Enterococcus faecalis (E. faecalis) biofilm under conditions of starvation and in an alkaline environment and to explore the function of chemical composition changes in the resistance of the E. faecalis biofilm to an extreme environment.
27689418	1	7	dep	faecalis	238:245	arg1	faecalis					251:258	E. faecalis	248:258	E. faecalis	248:258	ABSTACT This study aimed to investigate the dynamic changes that occur in the chemical composition of an Enterococcus faecalis (E. faecalis) biofilm under conditions of starvation and in an alkaline environment and to explore the function of chemical composition changes in the resistance of the E. faecalis biofilm to an extreme environment.
27689418	2	8	theme	biofilm	510:516	arg1	model					518:522	an in vitro E. faecalis biofilm model	486:522	an in vitro E. faecalis biofilm model	486:522	This study established an in vitro E. faecalis biofilm model under starvation and in an alkaline environment.
27689418	0	9	from	Differences	0:10	arg1	composition					28:38	the chemical composition	15:38	the chemical composition of Enterococcus faecalis	15:63	Differences in the chemical composition of Enterococcus faecalis biofilm under conditions of starvation and alkalinity.
27689418	1	10	theme	biofilm	428:434	arg1	resistance					398:407	the resistance	394:407	the resistance of the E. faecalis biofilm to an extreme environment	394:460	ABSTACT This study aimed to investigate the dynamic changes that occur in the chemical composition of an Enterococcus faecalis (E. faecalis) biofilm under conditions of starvation and in an alkaline environment and to explore the function of chemical composition changes in the resistance of the E. faecalis biofilm to an extreme environment.
27689418	8	11	theme	environmental	1428:1440	arg1	pressures					1442:1450	environmental pressures	1428:1450	environmental pressures	1428:1450	These changes served as a defensive mechanism for E. faecalis against environmental pressures.
27689418	0	12	theme	starvation	93:102	arg1	conditions					79:88	conditions	79:88	conditions of starvation and alkalinity	79:117	Differences in the chemical composition of Enterococcus faecalis biofilm under conditions of starvation and alkalinity.
27689418	5	13	theme	9	1055:1055	arg1	values					1045:1050	pH values	1042:1050	pH values of 9 and 11	1042:1062	In addition, the percentage of water-soluble polysaccharides in culture medium with pH values of 9 and 11 gradually decreased.
27689418	6	14	theme	other	1217:1221	arg1	conditions					1223:1232	other conditions	1217:1232	other conditions	1217:1232	The level of the water-soluble polysaccharides in each milligram of dry weight of biofilm at pH 11 increased compared to that under other conditions.
27689418	1	15	theme	biofilm	261:267	arg1	composition					207:217	the chemical composition	194:217	the chemical composition of an Enterococcus faecalis (E. faecalis) biofilm under conditions of starvation and in an alkaline environment	194:329	ABSTACT This study aimed to investigate the dynamic changes that occur in the chemical composition of an Enterococcus faecalis (E. faecalis) biofilm under conditions of starvation and in an alkaline environment and to explore the function of chemical composition changes in the resistance of the E. faecalis biofilm to an extreme environment.
27689418	2	16	theme	E.	498:499	arg1	model					518:522	an in vitro E. faecalis biofilm model	486:522	an in vitro E. faecalis biofilm model	486:522	This study established an in vitro E. faecalis biofilm model under starvation and in an alkaline environment.
27689418	1	17	dep	ABSTACT	120:126	arg1	aimed					139:143	aimed	139:143	aimed to investigate the dynamic changes that occur in the chemical composition of an Enterococcus faecalis (E. faecalis) biofilm under conditions of starvation and in an alkaline environment and to explore the function of chemical composition changes in the resistance of the E. faecalis biofilm to an extreme environment	139:460	ABSTACT This study aimed to investigate the dynamic changes that occur in the chemical composition of an Enterococcus faecalis (E. faecalis) biofilm under conditions of starvation and in an alkaline environment and to explore the function of chemical composition changes in the resistance of the E. faecalis biofilm to an extreme environment.
27689418	5	18	with	medium	1030:1035	arg1	values					1045:1050	pH values	1042:1050	pH values of 9 and 11	1042:1062	In addition, the percentage of water-soluble polysaccharides in culture medium with pH values of 9 and 11 gradually decreased.
27689418	4	19	theme	medium	825:830	arg1	value					804:808	the pH value	797:808	the pH value of the culture medium	797:830	The results showed that, when the pH value of the culture medium was 11, the percentage of water-insoluble polysaccharides in the biofilm was significantly lower than under other conditions.
27689418	4	19	theme	medium	825:830	arg1	11					836:837	11	836:837	11	836:837	The results showed that, when the pH value of the culture medium was 11, the percentage of water-insoluble polysaccharides in the biofilm was significantly lower than under other conditions.
27689418	0	20	theme	alkalinity	108:117	arg1	conditions					79:88	conditions	79:88	conditions of starvation and alkalinity	79:117	Differences in the chemical composition of Enterococcus faecalis biofilm under conditions of starvation and alkalinity.
27689418	5	21	theme	11	1061:1062	arg1	values					1045:1050	pH values	1042:1050	pH values of 9 and 11	1042:1062	In addition, the percentage of water-soluble polysaccharides in culture medium with pH values of 9 and 11 gradually decreased.
27689418	4	22	theme	pH	801:802	arg1	value					804:808	the pH value	797:808	the pH value of the culture medium	797:830	The results showed that, when the pH value of the culture medium was 11, the percentage of water-insoluble polysaccharides in the biofilm was significantly lower than under other conditions.
27689418	4	22	theme	pH	801:802	arg1	11					836:837	11	836:837	11	836:837	The results showed that, when the pH value of the culture medium was 11, the percentage of water-insoluble polysaccharides in the biofilm was significantly lower than under other conditions.
27689418	1	23	theme	extreme	442:448	arg1	environment					450:460	an extreme environment	439:460	an extreme environment	439:460	ABSTACT This study aimed to investigate the dynamic changes that occur in the chemical composition of an Enterococcus faecalis (E. faecalis) biofilm under conditions of starvation and in an alkaline environment and to explore the function of chemical composition changes in the resistance of the E. faecalis biofilm to an extreme environment.
27689418	7	24	theme	faecalis	1308:1315	arg1	biofilm					1317:1323	E. faecalis biofilm	1305:1323	E. faecalis biofilm	1305:1323	The results from this study indicate that the chemical composition of E. faecalis biofilm changed in extreme environments.
27689418	7	25	theme	chemical	1281:1288	arg1	composition					1290:1300	the chemical composition	1277:1300	the chemical composition of E. faecalis biofilm	1277:1323	The results from this study indicate that the chemical composition of E. faecalis biofilm changed in extreme environments.
27689418	1	26	theme	chemical	362:369	arg1	changes					383:389	chemical composition changes	362:389	chemical composition changes in the resistance of the E. faecalis biofilm to an extreme environment	362:460	ABSTACT This study aimed to investigate the dynamic changes that occur in the chemical composition of an Enterococcus faecalis (E. faecalis) biofilm under conditions of starvation and in an alkaline environment and to explore the function of chemical composition changes in the resistance of the E. faecalis biofilm to an extreme environment.
27689418	6	27	theme	polysaccharides	1116:1130	arg1	level					1089:1093	The level	1085:1093	The level of the water-soluble polysaccharides in each milligram of dry weight of biofilm at pH 11	1085:1182	The level of the water-soluble polysaccharides in each milligram of dry weight of biofilm at pH 11 increased compared to that under other conditions.
27689418	6	28	from	milligram	1140:1148	arg1	pH					1178:1179	pH 11	1178:1182	pH 11	1178:1182	The level of the water-soluble polysaccharides in each milligram of dry weight of biofilm at pH 11 increased compared to that under other conditions.
27689418	3	29	theme	medium	664:669	arg1	value					617:621	the pH value	610:621	the pH value	610:621	During the formation of the biofilm, the pH value and nutritional condition of the culture medium were changed, and the changes in chemical composition were observed using biochemical measures.
27689418	3	29	theme	medium	664:669	arg1	condition					639:647	nutritional condition	627:647	nutritional condition	627:647	During the formation of the biofilm, the pH value and nutritional condition of the culture medium were changed, and the changes in chemical composition were observed using biochemical measures.
27689418	5	30	theme	polysaccharides	1003:1017	arg1	percentage					975:984	the percentage	971:984	the percentage of water-soluble polysaccharides in culture medium with pH values of 9 and 11	971:1062	In addition, the percentage of water-soluble polysaccharides in culture medium with pH values of 9 and 11 gradually decreased.
27689418	5	31	theme	pH	1042:1043	arg1	values					1045:1050	pH values	1042:1050	pH values of 9 and 11	1042:1062	In addition, the percentage of water-soluble polysaccharides in culture medium with pH values of 9 and 11 gradually decreased.
27689418	0	32	theme	chemical	19:26	arg1	composition					28:38	the chemical composition	15:38	the chemical composition of Enterococcus faecalis	15:63	Differences in the chemical composition of Enterococcus faecalis biofilm under conditions of starvation and alkalinity.
27689418	8	33	theme	defensive	1384:1392	arg1	mechanism					1394:1402	a defensive mechanism	1382:1402	a defensive mechanism for E. faecalis against environmental pressures	1382:1450	These changes served as a defensive mechanism for E. faecalis against environmental pressures.
27689418	8	33	theme	defensive	1384:1392	arg1	changes					1364:1370	These changes	1358:1370	These changes	1358:1370	These changes served as a defensive mechanism for E. faecalis against environmental pressures.
27689418	4	34	theme	polysaccharides	874:888	arg1	percentage					844:853	the percentage	840:853	the percentage of water-insoluble polysaccharides in the biofilm	840:903	The results showed that, when the pH value of the culture medium was 11, the percentage of water-insoluble polysaccharides in the biofilm was significantly lower than under other conditions.
27689418	4	34	theme	polysaccharides	874:888	arg1	lower					923:927	lower	923:927	lower	923:927	The results showed that, when the pH value of the culture medium was 11, the percentage of water-insoluble polysaccharides in the biofilm was significantly lower than under other conditions.
27689418	1	35	from	function	350:357	arg1	resistance					398:407	the resistance	394:407	the resistance of the E. faecalis biofilm to an extreme environment	394:460	ABSTACT This study aimed to investigate the dynamic changes that occur in the chemical composition of an Enterococcus faecalis (E. faecalis) biofilm under conditions of starvation and in an alkaline environment and to explore the function of chemical composition changes in the resistance of the E. faecalis biofilm to an extreme environment.
27689418	1	36	from	biofilm	261:267	arg1	environment					319:329	an alkaline environment	307:329	an alkaline environment	307:329	ABSTACT This study aimed to investigate the dynamic changes that occur in the chemical composition of an Enterococcus faecalis (E. faecalis) biofilm under conditions of starvation and in an alkaline environment and to explore the function of chemical composition changes in the resistance of the E. faecalis biofilm to an extreme environment.
27689418	1	37	theme	composition	371:381	arg1	changes					383:389	chemical composition changes	362:389	chemical composition changes in the resistance of the E. faecalis biofilm to an extreme environment	362:460	ABSTACT This study aimed to investigate the dynamic changes that occur in the chemical composition of an Enterococcus faecalis (E. faecalis) biofilm under conditions of starvation and in an alkaline environment and to explore the function of chemical composition changes in the resistance of the E. faecalis biofilm to an extreme environment.
27689418	1	38	theme	chemical	198:205	arg1	composition					207:217	the chemical composition	194:217	the chemical composition of an Enterococcus faecalis (E. faecalis) biofilm under conditions of starvation and in an alkaline environment	194:329	ABSTACT This study aimed to investigate the dynamic changes that occur in the chemical composition of an Enterococcus faecalis (E. faecalis) biofilm under conditions of starvation and in an alkaline environment and to explore the function of chemical composition changes in the resistance of the E. faecalis biofilm to an extreme environment.
27689418	1	39	theme	starvation	289:298	arg1	conditions					275:284	conditions	275:284	conditions of starvation	275:298	ABSTACT This study aimed to investigate the dynamic changes that occur in the chemical composition of an Enterococcus faecalis (E. faecalis) biofilm under conditions of starvation and in an alkaline environment and to explore the function of chemical composition changes in the resistance of the E. faecalis biofilm to an extreme environment.
27689418	1	40	theme	changes	383:389	arg1	function					350:357	the function	346:357	the function of chemical composition changes in the resistance of the E. faecalis biofilm to an extreme environment	346:460	ABSTACT This study aimed to investigate the dynamic changes that occur in the chemical composition of an Enterococcus faecalis (E. faecalis) biofilm under conditions of starvation and in an alkaline environment and to explore the function of chemical composition changes in the resistance of the E. faecalis biofilm to an extreme environment.
27689418	5	41	from	percentage	975:984	arg1	medium					1030:1035	culture medium	1022:1035	culture medium with pH values of 9 and 11	1022:1062	In addition, the percentage of water-soluble polysaccharides in culture medium with pH values of 9 and 11 gradually decreased.
27689418	3	42	theme	biochemical	745:755	arg1	measures					757:764	biochemical measures	745:764	biochemical measures	745:764	During the formation of the biofilm, the pH value and nutritional condition of the culture medium were changed, and the changes in chemical composition were observed using biochemical measures.
27689418	1	43	dep	E.	416:417	arg1	faecalis					419:426	faecalis	419:426	faecalis	419:426	ABSTACT This study aimed to investigate the dynamic changes that occur in the chemical composition of an Enterococcus faecalis (E. faecalis) biofilm under conditions of starvation and in an alkaline environment and to explore the function of chemical composition changes in the resistance of the E. faecalis biofilm to an extreme environment.
27689418	4	44	from	polysaccharides	874:888	arg1	biofilm					897:903	the biofilm	893:903	the biofilm	893:903	The results showed that, when the pH value of the culture medium was 11, the percentage of water-insoluble polysaccharides in the biofilm was significantly lower than under other conditions.
27689418	6	45	theme	water-soluble	1102:1114	arg1	polysaccharides					1116:1130	the water-soluble polysaccharides	1098:1130	the water-soluble polysaccharides	1098:1130	The level of the water-soluble polysaccharides in each milligram of dry weight of biofilm at pH 11 increased compared to that under other conditions.
27689418	1	46	from	composition	207:217	arg1	environment					319:329	an alkaline environment	307:329	an alkaline environment	307:329	ABSTACT This study aimed to investigate the dynamic changes that occur in the chemical composition of an Enterococcus faecalis (E. faecalis) biofilm under conditions of starvation and in an alkaline environment and to explore the function of chemical composition changes in the resistance of the E. faecalis biofilm to an extreme environment.
27689418	1	47	from	changes	383:389	arg1	resistance					398:407	the resistance	394:407	the resistance of the E. faecalis biofilm to an extreme environment	394:460	ABSTACT This study aimed to investigate the dynamic changes that occur in the chemical composition of an Enterococcus faecalis (E. faecalis) biofilm under conditions of starvation and in an alkaline environment and to explore the function of chemical composition changes in the resistance of the E. faecalis biofilm to an extreme environment.
27689418	0	48	theme	faecalis	56:63	arg1	composition					28:38	the chemical composition	15:38	the chemical composition of Enterococcus faecalis	15:63	Differences in the chemical composition of Enterococcus faecalis biofilm under conditions of starvation and alkalinity.
27689418	5	49	from	medium	1030:1035	arg1	percentage					975:984	the percentage	971:984	the percentage of water-soluble polysaccharides in culture medium with pH values of 9 and 11	971:1062	In addition, the percentage of water-soluble polysaccharides in culture medium with pH values of 9 and 11 gradually decreased.
27689418	3	50	theme	pH	614:615	arg1	value					617:621	the pH value	610:621	the pH value	610:621	During the formation of the biofilm, the pH value and nutritional condition of the culture medium were changed, and the changes in chemical composition were observed using biochemical measures.
27689418	7	51	theme	biofilm	1317:1323	arg1	composition					1290:1300	the chemical composition	1277:1300	the chemical composition of E. faecalis biofilm	1277:1323	The results from this study indicate that the chemical composition of E. faecalis biofilm changed in extreme environments.
27689418	0	52	theme	Enterococcus	43:54	arg1	faecalis					56:63	Enterococcus faecalis	43:63	Enterococcus faecalis	43:63	Differences in the chemical composition of Enterococcus faecalis biofilm under conditions of starvation and alkalinity.
27689418	2	53	theme	alkaline	551:558	arg1	environment					560:570	an alkaline environment	548:570	an alkaline environment	548:570	This study established an in vitro E. faecalis biofilm model under starvation and in an alkaline environment.
27689418	4	54	from	biofilm	897:903	arg1	percentage					844:853	the percentage	840:853	the percentage of water-insoluble polysaccharides in the biofilm	840:903	The results showed that, when the pH value of the culture medium was 11, the percentage of water-insoluble polysaccharides in the biofilm was significantly lower than under other conditions.
27689418	4	54	from	biofilm	897:903	arg1	lower					923:927	lower	923:927	lower	923:927	The results showed that, when the pH value of the culture medium was 11, the percentage of water-insoluble polysaccharides in the biofilm was significantly lower than under other conditions.
27689418	5	55	theme	water-soluble	989:1001	arg1	polysaccharides					1003:1017	water-soluble polysaccharides	989:1017	water-soluble polysaccharides in culture medium with pH values of 9 and 11	989:1062	In addition, the percentage of water-soluble polysaccharides in culture medium with pH values of 9 and 11 gradually decreased.
27689418	6	56	from	level	1089:1093	arg1	milligram					1140:1148	each milligram	1135:1148	each milligram of dry weight of biofilm at pH 11	1135:1182	The level of the water-soluble polysaccharides in each milligram of dry weight of biofilm at pH 11 increased compared to that under other conditions.
27689418	6	57	theme	dry	1153:1155	arg1	weight					1157:1162	dry weight	1153:1162	dry weight of biofilm at pH 11	1153:1182	The level of the water-soluble polysaccharides in each milligram of dry weight of biofilm at pH 11 increased compared to that under other conditions.
27689418	6	58	from	pH	1178:1179	arg1	milligram					1140:1148	each milligram	1135:1148	each milligram of dry weight of biofilm at pH 11	1135:1182	The level of the water-soluble polysaccharides in each milligram of dry weight of biofilm at pH 11 increased compared to that under other conditions.
27689418	6	58	from	pH	1178:1179	arg1	weight					1157:1162	dry weight	1153:1162	dry weight of biofilm at pH 11	1153:1182	The level of the water-soluble polysaccharides in each milligram of dry weight of biofilm at pH 11 increased compared to that under other conditions.
27689418	1	59	theme	alkaline	310:317	arg1	environment					319:329	an alkaline environment	307:329	an alkaline environment	307:329	ABSTACT This study aimed to investigate the dynamic changes that occur in the chemical composition of an Enterococcus faecalis (E. faecalis) biofilm under conditions of starvation and in an alkaline environment and to explore the function of chemical composition changes in the resistance of the E. faecalis biofilm to an extreme environment.
27689418	2	60	dep	in	489:490	arg1	vitro					492:496	vitro	492:496	vitro	492:496	This study established an in vitro E. faecalis biofilm model under starvation and in an alkaline environment.
27689418	4	61	from	percentage	844:853	arg1	biofilm					897:903	the biofilm	893:903	the biofilm	893:903	The results showed that, when the pH value of the culture medium was 11, the percentage of water-insoluble polysaccharides in the biofilm was significantly lower than under other conditions.
27689418	3	62	theme	biofilm	601:607	arg1	formation					584:592	the formation	580:592	the formation of the biofilm	580:607	During the formation of the biofilm, the pH value and nutritional condition of the culture medium were changed, and the changes in chemical composition were observed using biochemical measures.
27689418	7	63	theme	extreme	1336:1342	arg1	environments					1344:1355	extreme environments	1336:1355	extreme environments	1336:1355	The results from this study indicate that the chemical composition of E. faecalis biofilm changed in extreme environments.
27689418	7	64	from	study	1257:1261	arg1	results					1239:1245	The results	1235:1245	The results from this study	1235:1261	The results from this study indicate that the chemical composition of E. faecalis biofilm changed in extreme environments.
27689418	3	65	theme	nutritional	627:637	arg1	condition					639:647	nutritional condition	627:647	nutritional condition	627:647	During the formation of the biofilm, the pH value and nutritional condition of the culture medium were changed, and the changes in chemical composition were observed using biochemical measures.
27689418	7	66	theme	E.	1305:1306	arg1	biofilm					1317:1323	E. faecalis biofilm	1305:1323	E. faecalis biofilm	1305:1323	The results from this study indicate that the chemical composition of E. faecalis biofilm changed in extreme environments.
27689418	5	67	theme	culture	1022:1028	arg1	medium					1030:1035	culture medium	1022:1035	culture medium with pH values of 9 and 11	1022:1062	In addition, the percentage of water-soluble polysaccharides in culture medium with pH values of 9 and 11 gradually decreased.
27689418	4	68	theme	water-insoluble	858:872	arg1	polysaccharides					874:888	water-insoluble polysaccharides	858:888	water-insoluble polysaccharides in the biofilm	858:903	The results showed that, when the pH value of the culture medium was 11, the percentage of water-insoluble polysaccharides in the biofilm was significantly lower than under other conditions.
27689418	2	69	theme	in	489:490	arg1	model					518:522	an in vitro E. faecalis biofilm model	486:522	an in vitro E. faecalis biofilm model	486:522	This study established an in vitro E. faecalis biofilm model under starvation and in an alkaline environment.
27689418	1	70	theme	E.	416:417	arg1	biofilm					428:434	the E. faecalis biofilm	412:434	the E. faecalis biofilm	412:434	ABSTACT This study aimed to investigate the dynamic changes that occur in the chemical composition of an Enterococcus faecalis (E. faecalis) biofilm under conditions of starvation and in an alkaline environment and to explore the function of chemical composition changes in the resistance of the E. faecalis biofilm to an extreme environment.
27689418	6	71	theme	weight	1157:1162	arg1	milligram					1140:1148	each milligram	1135:1148	each milligram of dry weight of biofilm at pH 11	1135:1182	The level of the water-soluble polysaccharides in each milligram of dry weight of biofilm at pH 11 increased compared to that under other conditions.
27689418	1	72	theme	faecalis	238:245	arg1	biofilm					261:267	an Enterococcus faecalis (E. faecalis) biofilm	222:267	an Enterococcus faecalis (E. faecalis) biofilm under conditions of starvation and in an alkaline environment	222:329	ABSTACT This study aimed to investigate the dynamic changes that occur in the chemical composition of an Enterococcus faecalis (E. faecalis) biofilm under conditions of starvation and in an alkaline environment and to explore the function of chemical composition changes in the resistance of the E. faecalis biofilm to an extreme environment.
27689418	1	73	from	environment	319:329	arg1	composition					207:217	the chemical composition	194:217	the chemical composition of an Enterococcus faecalis (E. faecalis) biofilm under conditions of starvation and in an alkaline environment	194:329	ABSTACT This study aimed to investigate the dynamic changes that occur in the chemical composition of an Enterococcus faecalis (E. faecalis) biofilm under conditions of starvation and in an alkaline environment and to explore the function of chemical composition changes in the resistance of the E. faecalis biofilm to an extreme environment.
27689418	3	74	from	changes	693:699	arg1	composition					713:723	chemical composition	704:723	chemical composition	704:723	During the formation of the biofilm, the pH value and nutritional condition of the culture medium were changed, and the changes in chemical composition were observed using biochemical measures.
27689418	6	75	theme	biofilm	1167:1173	arg1	weight					1157:1162	dry weight	1153:1162	dry weight of biofilm at pH 11	1153:1182	The level of the water-soluble polysaccharides in each milligram of dry weight of biofilm at pH 11 increased compared to that under other conditions.
27689418	2	76	theme	faecalis	501:508	arg1	model					518:522	an in vitro E. faecalis biofilm model	486:522	an in vitro E. faecalis biofilm model	486:522	This study established an in vitro E. faecalis biofilm model under starvation and in an alkaline environment.
25601326	0	0	theme	Biological	73:82	arg1	-α-D-Glucans					5:16	-α-D-Glucans	5:16	(1→3)-α-D-Glucans from Aspergillus spp.: Structural Characterization and Biological Study on their Carboxymethylated Derivatives.	0:128	(1→3)-α-D-Glucans from Aspergillus spp.: Structural Characterization and Biological Study on their Carboxymethylated Derivatives.
25601326	0	0	theme	Biological	73:82	arg1	Study					84:88	Biological Study	73:88	Biological Study	73:88	(1→3)-α-D-Glucans from Aspergillus spp.: Structural Characterization and Biological Study on their Carboxymethylated Derivatives.
25601326	6	1	from	wentii	851:856	arg1	incubation					813:822	incubation	813:822	incubation with CM-α-D-glucan from A. wentii and A. niger	813:869	However, incubation with CM-α-D-glucan from A. wentii and A. niger increased (by ca. 50%) the viability of HSF cells.
25601326	9	2	theme	cytoskeleton	1315:1326	arg1	organization					1328:1339	cellular cytoskeleton organization	1306:1339	cellular cytoskeleton organization	1306:1339	Fluorescent staining revealed that CM-α-D-glucans exerted slight toxic effects on cell viability and no action on F-actin filaments of cellular cytoskeleton organization.
25601326	9	3	theme	Fluorescent	1171:1181	arg1	staining					1183:1190	Fluorescent staining	1171:1190	Fluorescent staining	1171:1190	Fluorescent staining revealed that CM-α-D-glucans exerted slight toxic effects on cell viability and no action on F-actin filaments of cellular cytoskeleton organization.
25601326	6	4	dep	50	889:890	arg1	ca.					885:887	ca.	885:887	ca.	885:887	However, incubation with CM-α-D-glucan from A. wentii and A. niger increased (by ca. 50%) the viability of HSF cells.
25601326	6	5	with	incubation	813:822	arg1	CM-α-D-glucan					829:841	CM-α-D-glucan	829:841	CM-α-D-glucan	829:841	However, incubation with CM-α-D-glucan from A. wentii and A. niger increased (by ca. 50%) the viability of HSF cells.
25601326	7	6	from	increase	947:954	arg1	viability					963:971	the viability	959:971	the viability	959:971	Moreover, an over 5-fold increase in the viability was found for Jurkat cells incubated with CM-α-D-glucans from A. fumigatus and A. nidulans.
25601326	7	7	theme	Jurkat	987:992	arg1	cells					994:998	Jurkat cells	987:998	Jurkat cells incubated with CM-α-D-glucans from A. fumigatus and A. nidulans	987:1062	Moreover, an over 5-fold increase in the viability was found for Jurkat cells incubated with CM-α-D-glucans from A. fumigatus and A. nidulans.
25601326	3	8	dep	lines	601:605	arg1	HeLa					613:616	HeLa	613:616	HeLa	613:616	After carboxymethylation (CM), the activity of (1→3)-α-D-glucans on three human cell lines (HSF, HeLa, and Jurkat) was assessed.
25601326	3	8	dep	lines	601:605	arg1	Jurkat					623:628	Jurkat	623:628	Jurkat	623:628	After carboxymethylation (CM), the activity of (1→3)-α-D-glucans on three human cell lines (HSF, HeLa, and Jurkat) was assessed.
25601326	3	8	dep	lines	601:605	arg1	HSF					608:610	HSF	608:610	HSF	608:610	After carboxymethylation (CM), the activity of (1→3)-α-D-glucans on three human cell lines (HSF, HeLa, and Jurkat) was assessed.
25601326	3	8	dep	lines	601:605	arg1	lines					601:605	three human cell lines	584:605	three human cell lines (HSF, HeLa, and Jurkat)	584:629	After carboxymethylation (CM), the activity of (1→3)-α-D-glucans on three human cell lines (HSF, HeLa, and Jurkat) was assessed.
25601326	4	9	theme	cytotoxic	665:673	arg1	action					704:709	Anti-proliferative, cytotoxic, and free radical scavenging action	645:709	Anti-proliferative, cytotoxic, and free radical scavenging action of CM-α-D-glucans	645:727	Anti-proliferative, cytotoxic, and free radical scavenging action of CM-α-D-glucans was analysed.
25601326	2	10	dep	investigations	314:327	arg1	analysis					371:378	composition analysis	359:378	composition analysis	359:378	The chemical and spectroscopic investigations (immunofluorescent labelling, composition analysis, methylation analysis, FTIR, and 1H NMR) indicated that the ASPs were polymers composed almost exclusively of (1→3)-linked α-D-glucose.
25601326	2	10	dep	investigations	314:327	arg1	investigations					314:327	The chemical and spectroscopic investigations	283:327	The chemical and spectroscopic investigations (immunofluorescent labelling, composition analysis, methylation analysis, FTIR, and 1H NMR)	283:419	The chemical and spectroscopic investigations (immunofluorescent labelling, composition analysis, methylation analysis, FTIR, and 1H NMR) indicated that the ASPs were polymers composed almost exclusively of (1→3)-linked α-D-glucose.
25601326	2	10	dep	investigations	314:327	arg1	NMR					416:418	1H NMR	413:418	1H NMR	413:418	The chemical and spectroscopic investigations (immunofluorescent labelling, composition analysis, methylation analysis, FTIR, and 1H NMR) indicated that the ASPs were polymers composed almost exclusively of (1→3)-linked α-D-glucose.
25601326	2	10	dep	investigations	314:327	arg1	labelling					348:356	immunofluorescent labelling	330:356	immunofluorescent labelling	330:356	The chemical and spectroscopic investigations (immunofluorescent labelling, composition analysis, methylation analysis, FTIR, and 1H NMR) indicated that the ASPs were polymers composed almost exclusively of (1→3)-linked α-D-glucose.
25601326	2	10	dep	investigations	314:327	arg1	analysis					393:400	methylation analysis	381:400	methylation analysis	381:400	The chemical and spectroscopic investigations (immunofluorescent labelling, composition analysis, methylation analysis, FTIR, and 1H NMR) indicated that the ASPs were polymers composed almost exclusively of (1→3)-linked α-D-glucose.
25601326	2	10	dep	investigations	314:327	arg1	FTIR					403:406	FTIR	403:406	FTIR	403:406	The chemical and spectroscopic investigations (immunofluorescent labelling, composition analysis, methylation analysis, FTIR, and 1H NMR) indicated that the ASPs were polymers composed almost exclusively of (1→3)-linked α-D-glucose.
25601326	3	11	from	activity	551:558	arg1	HeLa					613:616	HeLa	613:616	HeLa	613:616	After carboxymethylation (CM), the activity of (1→3)-α-D-glucans on three human cell lines (HSF, HeLa, and Jurkat) was assessed.
25601326	3	11	from	activity	551:558	arg1	Jurkat					623:628	Jurkat	623:628	Jurkat	623:628	After carboxymethylation (CM), the activity of (1→3)-α-D-glucans on three human cell lines (HSF, HeLa, and Jurkat) was assessed.
25601326	3	11	from	activity	551:558	arg1	HSF					608:610	HSF	608:610	HSF	608:610	After carboxymethylation (CM), the activity of (1→3)-α-D-glucans on three human cell lines (HSF, HeLa, and Jurkat) was assessed.
25601326	3	11	from	activity	551:558	arg1	lines					601:605	three human cell lines	584:605	three human cell lines (HSF, HeLa, and Jurkat)	584:629	After carboxymethylation (CM), the activity of (1→3)-α-D-glucans on three human cell lines (HSF, HeLa, and Jurkat) was assessed.
25601326	9	12	theme	organization	1328:1339	arg1	filaments					1293:1301	F-actin filaments	1285:1301	F-actin filaments of cellular cytoskeleton organization	1285:1339	Fluorescent staining revealed that CM-α-D-glucans exerted slight toxic effects on cell viability and no action on F-actin filaments of cellular cytoskeleton organization.
25601326	0	13	theme	Carboxymethylated	99:115	arg1	Derivatives					117:127	their Carboxymethylated Derivatives	93:127	their Carboxymethylated Derivatives	93:127	(1→3)-α-D-Glucans from Aspergillus spp.: Structural Characterization and Biological Study on their Carboxymethylated Derivatives.
25601326	0	14	from	spp.	35:38	arg1	-α-D-Glucans					5:16	-α-D-Glucans	5:16	(1→3)-α-D-Glucans from Aspergillus spp.: Structural Characterization and Biological Study on their Carboxymethylated Derivatives.	0:128	(1→3)-α-D-Glucans from Aspergillus spp.: Structural Characterization and Biological Study on their Carboxymethylated Derivatives.
25601326	0	14	from	spp.	35:38	arg1	Characterization					52:67	Structural Characterization	41:67	Structural Characterization	41:67	(1→3)-α-D-Glucans from Aspergillus spp.: Structural Characterization and Biological Study on their Carboxymethylated Derivatives.
25601326	0	14	from	spp.	35:38	arg1	Study					84:88	Biological Study	73:88	Biological Study	73:88	(1→3)-α-D-Glucans from Aspergillus spp.: Structural Characterization and Biological Study on their Carboxymethylated Derivatives.
25601326	9	15	from	effects	1242:1248	arg1	viability					1258:1266	cell viability	1253:1266	cell viability	1253:1266	Fluorescent staining revealed that CM-α-D-glucans exerted slight toxic effects on cell viability and no action on F-actin filaments of cellular cytoskeleton organization.
25601326	9	15	from	effects	1242:1248	arg1	action					1275:1280	no action	1272:1280	no action	1272:1280	Fluorescent staining revealed that CM-α-D-glucans exerted slight toxic effects on cell viability and no action on F-actin filaments of cellular cytoskeleton organization.
25601326	9	15	from	effects	1242:1248	arg1	filaments					1293:1301	F-actin filaments	1285:1301	F-actin filaments of cellular cytoskeleton organization	1285:1339	Fluorescent staining revealed that CM-α-D-glucans exerted slight toxic effects on cell viability and no action on F-actin filaments of cellular cytoskeleton organization.
25601326	4	16	theme	free	680:683	arg1	action					704:709	Anti-proliferative, cytotoxic, and free radical scavenging action	645:709	Anti-proliferative, cytotoxic, and free radical scavenging action of CM-α-D-glucans	645:727	Anti-proliferative, cytotoxic, and free radical scavenging action of CM-α-D-glucans was analysed.
25601326	8	17	theme	Aspergillus	1106:1116	arg1	species					1118:1124	the tested Aspergillus species	1095:1124	the tested Aspergillus species	1095:1124	The CM-(1→3)-α-D-glucans from the tested Aspergillus species expressed no free radical scavenging action.
25601326	3	18	dep	-α-D-glucans	568:579	arg1	1→3					564:566	1→3	564:566	1→3	564:566	After carboxymethylation (CM), the activity of (1→3)-α-D-glucans on three human cell lines (HSF, HeLa, and Jurkat) was assessed.
25601326	5	19	theme	cellular	783:790	arg1	metabolism					792:801	cellular metabolism	783:801	cellular metabolism	783:801	All the tested CM-α-D-glucans decreased cellular metabolism.
25601326	8	20	theme	CM-	1069:1071	arg1	-α-D-glucans					1077:1088	The CM-(1→3)-α-D-glucans	1065:1088	The CM-(1→3)-α-D-glucans from the tested Aspergillus species	1065:1124	The CM-(1→3)-α-D-glucans from the tested Aspergillus species expressed no free radical scavenging action.
25601326	2	21	link	-linked	495:501	arg1	α-D-glucose					503:513	(1→3)-linked α-D-glucose	490:513	(1→3)-linked α-D-glucose	490:513	The chemical and spectroscopic investigations (immunofluorescent labelling, composition analysis, methylation analysis, FTIR, and 1H NMR) indicated that the ASPs were polymers composed almost exclusively of (1→3)-linked α-D-glucose.
25601326	2	22	theme	composition	359:369	arg1	analysis					371:378	composition analysis	359:378	composition analysis	359:378	The chemical and spectroscopic investigations (immunofluorescent labelling, composition analysis, methylation analysis, FTIR, and 1H NMR) indicated that the ASPs were polymers composed almost exclusively of (1→3)-linked α-D-glucose.
25601326	2	22	theme	composition	359:369	arg1	investigations					314:327	The chemical and spectroscopic investigations	283:327	The chemical and spectroscopic investigations (immunofluorescent labelling, composition analysis, methylation analysis, FTIR, and 1H NMR)	283:419	The chemical and spectroscopic investigations (immunofluorescent labelling, composition analysis, methylation analysis, FTIR, and 1H NMR) indicated that the ASPs were polymers composed almost exclusively of (1→3)-linked α-D-glucose.
25601326	2	23	theme	1H	413:414	arg1	NMR					416:418	1H NMR	413:418	1H NMR	413:418	The chemical and spectroscopic investigations (immunofluorescent labelling, composition analysis, methylation analysis, FTIR, and 1H NMR) indicated that the ASPs were polymers composed almost exclusively of (1→3)-linked α-D-glucose.
25601326	2	23	theme	1H	413:414	arg1	investigations					314:327	The chemical and spectroscopic investigations	283:327	The chemical and spectroscopic investigations (immunofluorescent labelling, composition analysis, methylation analysis, FTIR, and 1H NMR)	283:419	The chemical and spectroscopic investigations (immunofluorescent labelling, composition analysis, methylation analysis, FTIR, and 1H NMR) indicated that the ASPs were polymers composed almost exclusively of (1→3)-linked α-D-glucose.
25601326	9	24	theme	slight	1229:1234	arg1	effects					1242:1248	slight toxic effects	1229:1248	slight toxic effects on cell viability and no action on F-actin filaments of cellular cytoskeleton organization	1229:1339	Fluorescent staining revealed that CM-α-D-glucans exerted slight toxic effects on cell viability and no action on F-actin filaments of cellular cytoskeleton organization.
25601326	3	25	theme	cell	596:599	arg1	HeLa					613:616	HeLa	613:616	HeLa	613:616	After carboxymethylation (CM), the activity of (1→3)-α-D-glucans on three human cell lines (HSF, HeLa, and Jurkat) was assessed.
25601326	3	25	theme	cell	596:599	arg1	Jurkat					623:628	Jurkat	623:628	Jurkat	623:628	After carboxymethylation (CM), the activity of (1→3)-α-D-glucans on three human cell lines (HSF, HeLa, and Jurkat) was assessed.
25601326	3	25	theme	cell	596:599	arg1	HSF					608:610	HSF	608:610	HSF	608:610	After carboxymethylation (CM), the activity of (1→3)-α-D-glucans on three human cell lines (HSF, HeLa, and Jurkat) was assessed.
25601326	3	25	theme	cell	596:599	arg1	lines					601:605	three human cell lines	584:605	three human cell lines (HSF, HeLa, and Jurkat)	584:629	After carboxymethylation (CM), the activity of (1→3)-α-D-glucans on three human cell lines (HSF, HeLa, and Jurkat) was assessed.
25601326	1	26	theme	cell	191:194	arg1	wall					196:199	the cell wall	187:199	the cell wall of four Aspergillus species (A. fumigatus, A. nidulans, A. niger, and A. wentii)	187:280	Alkali-soluble polysaccharides (ASPs) were isolated from the cell wall of four Aspergillus species (A. fumigatus, A. nidulans, A. niger, and A. wentii).
25601326	8	27	theme	1→3	1073:1075	arg1	-α-D-glucans					1077:1088	The CM-(1→3)-α-D-glucans	1065:1088	The CM-(1→3)-α-D-glucans from the tested Aspergillus species	1065:1124	The CM-(1→3)-α-D-glucans from the tested Aspergillus species expressed no free radical scavenging action.
25601326	9	28	theme	toxic	1236:1240	arg1	effects					1242:1248	slight toxic effects	1229:1248	slight toxic effects on cell viability and no action on F-actin filaments of cellular cytoskeleton organization	1229:1339	Fluorescent staining revealed that CM-α-D-glucans exerted slight toxic effects on cell viability and no action on F-actin filaments of cellular cytoskeleton organization.
25601326	2	29	theme	methylation	381:391	arg1	analysis					393:400	methylation analysis	381:400	methylation analysis	381:400	The chemical and spectroscopic investigations (immunofluorescent labelling, composition analysis, methylation analysis, FTIR, and 1H NMR) indicated that the ASPs were polymers composed almost exclusively of (1→3)-linked α-D-glucose.
25601326	2	29	theme	methylation	381:391	arg1	investigations					314:327	The chemical and spectroscopic investigations	283:327	The chemical and spectroscopic investigations (immunofluorescent labelling, composition analysis, methylation analysis, FTIR, and 1H NMR)	283:419	The chemical and spectroscopic investigations (immunofluorescent labelling, composition analysis, methylation analysis, FTIR, and 1H NMR) indicated that the ASPs were polymers composed almost exclusively of (1→3)-linked α-D-glucose.
25601326	2	30	theme	-linked	495:501	arg1	α-D-glucose					503:513	(1→3)-linked α-D-glucose	490:513	(1→3)-linked α-D-glucose	490:513	The chemical and spectroscopic investigations (immunofluorescent labelling, composition analysis, methylation analysis, FTIR, and 1H NMR) indicated that the ASPs were polymers composed almost exclusively of (1→3)-linked α-D-glucose.
25601326	4	31	theme	Anti-proliferative	645:662	arg1	action					704:709	Anti-proliferative, cytotoxic, and free radical scavenging action	645:709	Anti-proliferative, cytotoxic, and free radical scavenging action of CM-α-D-glucans	645:727	Anti-proliferative, cytotoxic, and free radical scavenging action of CM-α-D-glucans was analysed.
25601326	7	32	theme	5-fold	940:945	arg1	increase					947:954	an over 5-fold increase	932:954	an over 5-fold increase in the viability	932:971	Moreover, an over 5-fold increase in the viability was found for Jurkat cells incubated with CM-α-D-glucans from A. fumigatus and A. nidulans.
25601326	1	33	attach	isolated	173:180	arg2	ASPs					162:165	ASPs	162:165	ASPs	162:165	Alkali-soluble polysaccharides (ASPs) were isolated from the cell wall of four Aspergillus species (A. fumigatus, A. nidulans, A. niger, and A. wentii).
25601326	1	33	attach	isolated	173:180	arg2	polysaccharides					145:159	Alkali-soluble polysaccharides	130:159	Alkali-soluble polysaccharides (ASPs)	130:166	Alkali-soluble polysaccharides (ASPs) were isolated from the cell wall of four Aspergillus species (A. fumigatus, A. nidulans, A. niger, and A. wentii).
25601326	1	33	attach	isolated	173:180	arg1	wall					196:199	the cell wall	187:199	the cell wall of four Aspergillus species (A. fumigatus, A. nidulans, A. niger, and A. wentii)	187:280	Alkali-soluble polysaccharides (ASPs) were isolated from the cell wall of four Aspergillus species (A. fumigatus, A. nidulans, A. niger, and A. wentii).
25601326	5	34	theme	tested	751:756	arg1	CM-α-D-glucans					758:771	All the tested CM-α-D-glucans	743:771	All the tested CM-α-D-glucans	743:771	All the tested CM-α-D-glucans decreased cellular metabolism.
25601326	9	35	theme	cell	1253:1256	arg1	viability					1258:1266	cell viability	1253:1266	cell viability	1253:1266	Fluorescent staining revealed that CM-α-D-glucans exerted slight toxic effects on cell viability and no action on F-actin filaments of cellular cytoskeleton organization.
25601326	9	36	theme	cellular	1306:1313	arg1	organization					1328:1339	cellular cytoskeleton organization	1306:1339	cellular cytoskeleton organization	1306:1339	Fluorescent staining revealed that CM-α-D-glucans exerted slight toxic effects on cell viability and no action on F-actin filaments of cellular cytoskeleton organization.
25601326	1	37	dep	species	221:227	arg1	wentii					274:279	A. wentii	271:279	A. wentii	271:279	Alkali-soluble polysaccharides (ASPs) were isolated from the cell wall of four Aspergillus species (A. fumigatus, A. nidulans, A. niger, and A. wentii).
25601326	1	37	dep	species	221:227	arg1	fumigatus					233:241	A. fumigatus	230:241	A. fumigatus	230:241	Alkali-soluble polysaccharides (ASPs) were isolated from the cell wall of four Aspergillus species (A. fumigatus, A. nidulans, A. niger, and A. wentii).
25601326	1	37	dep	species	221:227	arg1	nidulans					247:254	A. nidulans	244:254	A. nidulans	244:254	Alkali-soluble polysaccharides (ASPs) were isolated from the cell wall of four Aspergillus species (A. fumigatus, A. nidulans, A. niger, and A. wentii).
25601326	1	37	dep	species	221:227	arg1	niger					260:264	A. niger	257:264	A. niger	257:264	Alkali-soluble polysaccharides (ASPs) were isolated from the cell wall of four Aspergillus species (A. fumigatus, A. nidulans, A. niger, and A. wentii).
25601326	4	38	theme	CM-α-D-glucans	714:727	arg1	action					704:709	Anti-proliferative, cytotoxic, and free radical scavenging action	645:709	Anti-proliferative, cytotoxic, and free radical scavenging action of CM-α-D-glucans	645:727	Anti-proliferative, cytotoxic, and free radical scavenging action of CM-α-D-glucans was analysed.
25601326	1	39	theme	Aspergillus	209:219	arg1	species					221:227	four Aspergillus species	204:227	four Aspergillus species (A. fumigatus, A. nidulans, A. niger, and A. wentii)	204:280	Alkali-soluble polysaccharides (ASPs) were isolated from the cell wall of four Aspergillus species (A. fumigatus, A. nidulans, A. niger, and A. wentii).
25601326	3	40	theme	-α-D-glucans	568:579	arg1	activity					551:558	the activity	547:558	the activity of (1→3)-α-D-glucans on three human cell lines (HSF, HeLa, and Jurkat)	547:629	After carboxymethylation (CM), the activity of (1→3)-α-D-glucans on three human cell lines (HSF, HeLa, and Jurkat) was assessed.
25601326	8	41	from	species	1118:1124	arg1	-α-D-glucans					1077:1088	The CM-(1→3)-α-D-glucans	1065:1088	The CM-(1→3)-α-D-glucans from the tested Aspergillus species	1065:1124	The CM-(1→3)-α-D-glucans from the tested Aspergillus species expressed no free radical scavenging action.
25601326	2	42	theme	spectroscopic	300:312	arg1	analysis					371:378	composition analysis	359:378	composition analysis	359:378	The chemical and spectroscopic investigations (immunofluorescent labelling, composition analysis, methylation analysis, FTIR, and 1H NMR) indicated that the ASPs were polymers composed almost exclusively of (1→3)-linked α-D-glucose.
25601326	2	42	theme	spectroscopic	300:312	arg1	investigations					314:327	The chemical and spectroscopic investigations	283:327	The chemical and spectroscopic investigations (immunofluorescent labelling, composition analysis, methylation analysis, FTIR, and 1H NMR)	283:419	The chemical and spectroscopic investigations (immunofluorescent labelling, composition analysis, methylation analysis, FTIR, and 1H NMR) indicated that the ASPs were polymers composed almost exclusively of (1→3)-linked α-D-glucose.
25601326	2	42	theme	spectroscopic	300:312	arg1	NMR					416:418	1H NMR	413:418	1H NMR	413:418	The chemical and spectroscopic investigations (immunofluorescent labelling, composition analysis, methylation analysis, FTIR, and 1H NMR) indicated that the ASPs were polymers composed almost exclusively of (1→3)-linked α-D-glucose.
25601326	2	42	theme	spectroscopic	300:312	arg1	labelling					348:356	immunofluorescent labelling	330:356	immunofluorescent labelling	330:356	The chemical and spectroscopic investigations (immunofluorescent labelling, composition analysis, methylation analysis, FTIR, and 1H NMR) indicated that the ASPs were polymers composed almost exclusively of (1→3)-linked α-D-glucose.
25601326	2	42	theme	spectroscopic	300:312	arg1	analysis					393:400	methylation analysis	381:400	methylation analysis	381:400	The chemical and spectroscopic investigations (immunofluorescent labelling, composition analysis, methylation analysis, FTIR, and 1H NMR) indicated that the ASPs were polymers composed almost exclusively of (1→3)-linked α-D-glucose.
25601326	2	42	theme	spectroscopic	300:312	arg1	FTIR					403:406	FTIR	403:406	FTIR	403:406	The chemical and spectroscopic investigations (immunofluorescent labelling, composition analysis, methylation analysis, FTIR, and 1H NMR) indicated that the ASPs were polymers composed almost exclusively of (1→3)-linked α-D-glucose.
25601326	0	43	from	Characterization	52:67	arg1	Derivatives					117:127	their Carboxymethylated Derivatives	93:127	their Carboxymethylated Derivatives	93:127	(1→3)-α-D-Glucans from Aspergillus spp.: Structural Characterization and Biological Study on their Carboxymethylated Derivatives.
25601326	9	44	theme	no	1272:1273	arg1	action					1275:1280	no action	1272:1280	no action	1272:1280	Fluorescent staining revealed that CM-α-D-glucans exerted slight toxic effects on cell viability and no action on F-actin filaments of cellular cytoskeleton organization.
25601326	8	45	theme	scavenging	1152:1161	arg1	action					1163:1168	no free radical scavenging action	1136:1168	no free radical scavenging action	1136:1168	The CM-(1→3)-α-D-glucans from the tested Aspergillus species expressed no free radical scavenging action.
25601326	4	46	theme	scavenging	693:702	arg1	action					704:709	Anti-proliferative, cytotoxic, and free radical scavenging action	645:709	Anti-proliferative, cytotoxic, and free radical scavenging action of CM-α-D-glucans	645:727	Anti-proliferative, cytotoxic, and free radical scavenging action of CM-α-D-glucans was analysed.
25601326	0	47	dep	-α-D-Glucans	5:16	arg1	-α-D-Glucans					5:16	-α-D-Glucans	5:16	(1→3)-α-D-Glucans from Aspergillus spp.: Structural Characterization and Biological Study on their Carboxymethylated Derivatives.	0:128	(1→3)-α-D-Glucans from Aspergillus spp.: Structural Characterization and Biological Study on their Carboxymethylated Derivatives.
25601326	0	47	dep	-α-D-Glucans	5:16	arg1	1→3					1:3	1→3	1:3	1→3	1:3	(1→3)-α-D-Glucans from Aspergillus spp.: Structural Characterization and Biological Study on their Carboxymethylated Derivatives.
25601326	0	47	dep	-α-D-Glucans	5:16	arg1	Study					84:88	Biological Study	73:88	Biological Study	73:88	(1→3)-α-D-Glucans from Aspergillus spp.: Structural Characterization and Biological Study on their Carboxymethylated Derivatives.
25601326	0	47	dep	-α-D-Glucans	5:16	arg1	Characterization					52:67	Structural Characterization	41:67	Structural Characterization	41:67	(1→3)-α-D-Glucans from Aspergillus spp.: Structural Characterization and Biological Study on their Carboxymethylated Derivatives.
25601326	6	48	theme	cells	915:919	arg1	viability					898:906	the viability	894:906	the viability of HSF cells	894:919	However, incubation with CM-α-D-glucan from A. wentii and A. niger increased (by ca. 50%) the viability of HSF cells.
25601326	1	49	theme	Alkali-soluble	130:143	arg1	ASPs					162:165	ASPs	162:165	ASPs	162:165	Alkali-soluble polysaccharides (ASPs) were isolated from the cell wall of four Aspergillus species (A. fumigatus, A. nidulans, A. niger, and A. wentii).
25601326	1	49	theme	Alkali-soluble	130:143	arg1	polysaccharides					145:159	Alkali-soluble polysaccharides	130:159	Alkali-soluble polysaccharides (ASPs)	130:166	Alkali-soluble polysaccharides (ASPs) were isolated from the cell wall of four Aspergillus species (A. fumigatus, A. nidulans, A. niger, and A. wentii).
25601326	1	50	theme	species	221:227	arg1	wall					196:199	the cell wall	187:199	the cell wall of four Aspergillus species (A. fumigatus, A. nidulans, A. niger, and A. wentii)	187:280	Alkali-soluble polysaccharides (ASPs) were isolated from the cell wall of four Aspergillus species (A. fumigatus, A. nidulans, A. niger, and A. wentii).
25601326	6	51	from	niger	865:869	arg1	incubation					813:822	incubation	813:822	incubation with CM-α-D-glucan from A. wentii and A. niger	813:869	However, incubation with CM-α-D-glucan from A. wentii and A. niger increased (by ca. 50%) the viability of HSF cells.
25601326	3	52	theme	human	590:594	arg1	HeLa					613:616	HeLa	613:616	HeLa	613:616	After carboxymethylation (CM), the activity of (1→3)-α-D-glucans on three human cell lines (HSF, HeLa, and Jurkat) was assessed.
25601326	3	52	theme	human	590:594	arg1	Jurkat					623:628	Jurkat	623:628	Jurkat	623:628	After carboxymethylation (CM), the activity of (1→3)-α-D-glucans on three human cell lines (HSF, HeLa, and Jurkat) was assessed.
25601326	3	52	theme	human	590:594	arg1	HSF					608:610	HSF	608:610	HSF	608:610	After carboxymethylation (CM), the activity of (1→3)-α-D-glucans on three human cell lines (HSF, HeLa, and Jurkat) was assessed.
25601326	3	52	theme	human	590:594	arg1	lines					601:605	three human cell lines	584:605	three human cell lines (HSF, HeLa, and Jurkat)	584:629	After carboxymethylation (CM), the activity of (1→3)-α-D-glucans on three human cell lines (HSF, HeLa, and Jurkat) was assessed.
25601326	4	53	theme	radical	685:691	arg1	action					704:709	Anti-proliferative, cytotoxic, and free radical scavenging action	645:709	Anti-proliferative, cytotoxic, and free radical scavenging action of CM-α-D-glucans	645:727	Anti-proliferative, cytotoxic, and free radical scavenging action of CM-α-D-glucans was analysed.
25601326	9	54	theme	F-actin	1285:1291	arg1	filaments					1293:1301	F-actin filaments	1285:1301	F-actin filaments of cellular cytoskeleton organization	1285:1339	Fluorescent staining revealed that CM-α-D-glucans exerted slight toxic effects on cell viability and no action on F-actin filaments of cellular cytoskeleton organization.
25601326	6	55	dep	increased	871:879	arg1	%					891:891	ca. 50%	885:891	ca. 50%	885:891	However, incubation with CM-α-D-glucan from A. wentii and A. niger increased (by ca. 50%) the viability of HSF cells.
25601326	2	56	theme	chemical	287:294	arg1	analysis					371:378	composition analysis	359:378	composition analysis	359:378	The chemical and spectroscopic investigations (immunofluorescent labelling, composition analysis, methylation analysis, FTIR, and 1H NMR) indicated that the ASPs were polymers composed almost exclusively of (1→3)-linked α-D-glucose.
25601326	2	56	theme	chemical	287:294	arg1	investigations					314:327	The chemical and spectroscopic investigations	283:327	The chemical and spectroscopic investigations (immunofluorescent labelling, composition analysis, methylation analysis, FTIR, and 1H NMR)	283:419	The chemical and spectroscopic investigations (immunofluorescent labelling, composition analysis, methylation analysis, FTIR, and 1H NMR) indicated that the ASPs were polymers composed almost exclusively of (1→3)-linked α-D-glucose.
25601326	2	56	theme	chemical	287:294	arg1	NMR					416:418	1H NMR	413:418	1H NMR	413:418	The chemical and spectroscopic investigations (immunofluorescent labelling, composition analysis, methylation analysis, FTIR, and 1H NMR) indicated that the ASPs were polymers composed almost exclusively of (1→3)-linked α-D-glucose.
25601326	2	56	theme	chemical	287:294	arg1	labelling					348:356	immunofluorescent labelling	330:356	immunofluorescent labelling	330:356	The chemical and spectroscopic investigations (immunofluorescent labelling, composition analysis, methylation analysis, FTIR, and 1H NMR) indicated that the ASPs were polymers composed almost exclusively of (1→3)-linked α-D-glucose.
25601326	2	56	theme	chemical	287:294	arg1	analysis					393:400	methylation analysis	381:400	methylation analysis	381:400	The chemical and spectroscopic investigations (immunofluorescent labelling, composition analysis, methylation analysis, FTIR, and 1H NMR) indicated that the ASPs were polymers composed almost exclusively of (1→3)-linked α-D-glucose.
25601326	2	56	theme	chemical	287:294	arg1	FTIR					403:406	FTIR	403:406	FTIR	403:406	The chemical and spectroscopic investigations (immunofluorescent labelling, composition analysis, methylation analysis, FTIR, and 1H NMR) indicated that the ASPs were polymers composed almost exclusively of (1→3)-linked α-D-glucose.
25601326	7	57	dep	5-fold	940:945	arg1	over					935:938	over	935:938	over	935:938	Moreover, an over 5-fold increase in the viability was found for Jurkat cells incubated with CM-α-D-glucans from A. fumigatus and A. nidulans.
25601326	0	58	from	Study	84:88	arg1	Derivatives					117:127	their Carboxymethylated Derivatives	93:127	their Carboxymethylated Derivatives	93:127	(1→3)-α-D-Glucans from Aspergillus spp.: Structural Characterization and Biological Study on their Carboxymethylated Derivatives.
25601326	8	59	theme	radical	1144:1150	arg1	scavenging					1152:1161	no free radical scavenging	1136:1161	no free radical scavenging action	1136:1168	The CM-(1→3)-α-D-glucans from the tested Aspergillus species expressed no free radical scavenging action.
25601326	8	60	theme	free	1139:1142	arg1	scavenging					1152:1161	no free radical scavenging	1136:1161	no free radical scavenging action	1136:1168	The CM-(1→3)-α-D-glucans from the tested Aspergillus species expressed no free radical scavenging action.
25601326	8	61	theme	tested	1099:1104	arg1	species					1118:1124	the tested Aspergillus species	1095:1124	the tested Aspergillus species	1095:1124	The CM-(1→3)-α-D-glucans from the tested Aspergillus species expressed no free radical scavenging action.
25601326	6	62	theme	HSF	911:913	arg1	cells					915:919	HSF cells	911:919	HSF cells	911:919	However, incubation with CM-α-D-glucan from A. wentii and A. niger increased (by ca. 50%) the viability of HSF cells.
25601326	0	63	theme	Structural	41:50	arg1	-α-D-Glucans					5:16	-α-D-Glucans	5:16	(1→3)-α-D-Glucans from Aspergillus spp.: Structural Characterization and Biological Study on their Carboxymethylated Derivatives.	0:128	(1→3)-α-D-Glucans from Aspergillus spp.: Structural Characterization and Biological Study on their Carboxymethylated Derivatives.
25601326	0	63	theme	Structural	41:50	arg1	Characterization					52:67	Structural Characterization	41:67	Structural Characterization	41:67	(1→3)-α-D-Glucans from Aspergillus spp.: Structural Characterization and Biological Study on their Carboxymethylated Derivatives.
25601326	2	64	theme	immunofluorescent	330:346	arg1	investigations					314:327	The chemical and spectroscopic investigations	283:327	The chemical and spectroscopic investigations (immunofluorescent labelling, composition analysis, methylation analysis, FTIR, and 1H NMR)	283:419	The chemical and spectroscopic investigations (immunofluorescent labelling, composition analysis, methylation analysis, FTIR, and 1H NMR) indicated that the ASPs were polymers composed almost exclusively of (1→3)-linked α-D-glucose.
25601326	2	64	theme	immunofluorescent	330:346	arg1	labelling					348:356	immunofluorescent labelling	330:356	immunofluorescent labelling	330:356	The chemical and spectroscopic investigations (immunofluorescent labelling, composition analysis, methylation analysis, FTIR, and 1H NMR) indicated that the ASPs were polymers composed almost exclusively of (1→3)-linked α-D-glucose.
27869102	0	0	theme	gellan	101:106	arg1	gum					108:110	gellan gum	101:110	gellan gum	101:110	Novel injectable, self-gelling hydrogel-microparticle composites for bone regeneration consisting of gellan gum and calcium and magnesium carbonate microparticles.
27869102	2	1	theme	gellan	499:504	arg1	gum					506:508	the anionic polysaccharide gellan gum	472:508	the anionic polysaccharide gellan gum (GG)	472:513	In this study, carbonate microparticles containing different amounts of calcium (Ca) and magnesium (Mg) were added to solutions of the anionic polysaccharide gellan gum (GG) to crosslink GG by release of Ca2+ and Mg2+ from microparticles and thereby induce formation of hydrogel-microparticle composites.
27869102	2	1	theme	gellan	499:504	arg1	GG					511:512	GG	511:512	GG	511:512	In this study, carbonate microparticles containing different amounts of calcium (Ca) and magnesium (Mg) were added to solutions of the anionic polysaccharide gellan gum (GG) to crosslink GG by release of Ca2+ and Mg2+ from microparticles and thereby induce formation of hydrogel-microparticle composites.
27869102	7	2	theme	Ca	1142:1143	arg1	ratio					1148:1152	the Ca:Mg ratio	1138:1152	the Ca:Mg ratio of the ions added	1138:1170	The elemental Ca:Mg ratio in the mineral formed was similar to the Ca:Mg ratio of the ions added.
27869102	7	3	theme	elemental	1079:1087	arg1	ratio					1095:1099	The elemental Ca:Mg ratio	1075:1099	The elemental Ca:Mg ratio in the mineral formed	1075:1121	The elemental Ca:Mg ratio in the mineral formed was similar to the Ca:Mg ratio of the ions added.
27869102	7	3	theme	elemental	1079:1087	arg1	similar					1127:1133	similar	1127:1133	similar	1127:1133	The elemental Ca:Mg ratio in the mineral formed was similar to the Ca:Mg ratio of the ions added.
27869102	2	4	theme	different	392:400	arg1	Ca					422:423	Ca	422:423	Ca	422:423	In this study, carbonate microparticles containing different amounts of calcium (Ca) and magnesium (Mg) were added to solutions of the anionic polysaccharide gellan gum (GG) to crosslink GG by release of Ca2+ and Mg2+ from microparticles and thereby induce formation of hydrogel-microparticle composites.
27869102	2	4	theme	different	392:400	arg1	magnesium					430:438	magnesium	430:438	magnesium (Mg)	430:443	In this study, carbonate microparticles containing different amounts of calcium (Ca) and magnesium (Mg) were added to solutions of the anionic polysaccharide gellan gum (GG) to crosslink GG by release of Ca2+ and Mg2+ from microparticles and thereby induce formation of hydrogel-microparticle composites.
27869102	2	4	theme	different	392:400	arg1	calcium					413:419	calcium	413:419	calcium (Ca)	413:424	In this study, carbonate microparticles containing different amounts of calcium (Ca) and magnesium (Mg) were added to solutions of the anionic polysaccharide gellan gum (GG) to crosslink GG by release of Ca2+ and Mg2+ from microparticles and thereby induce formation of hydrogel-microparticle composites.
27869102	2	4	theme	different	392:400	arg1	amounts					402:408	different amounts	392:408	different amounts of calcium (Ca) and magnesium (Mg)	392:443	In this study, carbonate microparticles containing different amounts of calcium (Ca) and magnesium (Mg) were added to solutions of the anionic polysaccharide gellan gum (GG) to crosslink GG by release of Ca2+ and Mg2+ from microparticles and thereby induce formation of hydrogel-microparticle composites.
27869102	2	4	theme	different	392:400	arg1	Mg					441:442	Mg	441:442	Mg	441:442	In this study, carbonate microparticles containing different amounts of calcium (Ca) and magnesium (Mg) were added to solutions of the anionic polysaccharide gellan gum (GG) to crosslink GG by release of Ca2+ and Mg2+ from microparticles and thereby induce formation of hydrogel-microparticle composites.
27869102	1	5	theme	phase	276:280	arg1	incorporation					246:258	incorporation	246:258	incorporation of an inorganic phase in particle form	246:297	The suitability of hydrogel biomaterials for bone regeneration can be improved by incorporation of an inorganic phase in particle form, thus maintaining hydrogel injectability.
27869102	2	6	theme	polysaccharide	484:497	arg1	gum					506:508	the anionic polysaccharide gellan gum	472:508	the anionic polysaccharide gellan gum (GG)	472:513	In this study, carbonate microparticles containing different amounts of calcium (Ca) and magnesium (Mg) were added to solutions of the anionic polysaccharide gellan gum (GG) to crosslink GG by release of Ca2+ and Mg2+ from microparticles and thereby induce formation of hydrogel-microparticle composites.
27869102	2	6	theme	polysaccharide	484:497	arg1	GG					511:512	GG	511:512	GG	511:512	In this study, carbonate microparticles containing different amounts of calcium (Ca) and magnesium (Mg) were added to solutions of the anionic polysaccharide gellan gum (GG) to crosslink GG by release of Ca2+ and Mg2+ from microparticles and thereby induce formation of hydrogel-microparticle composites.
27869102	15	7	contain	containing	1765:1774	arg2	particles					1776:1784	particles	1776:1784	particles	1776:1784	Cell growth was highest on composites containing particles with an equimolar Ca:Mg ratio.
27869102	15	7	contain	containing	1765:1774	arg1	composites					1754:1763	composites	1754:1763	composites containing particles with an equimolar Ca:Mg ratio	1754:1814	Cell growth was highest on composites containing particles with an equimolar Ca:Mg ratio.
27869102	1	8	theme	hydrogel	183:190	arg1	biomaterials					192:203	hydrogel biomaterials	183:203	hydrogel biomaterials	183:203	The suitability of hydrogel biomaterials for bone regeneration can be improved by incorporation of an inorganic phase in particle form, thus maintaining hydrogel injectability.
27869102	7	9	from	ratio	1095:1099	arg1	mineral					1108:1114	mineral	1108:1114	mineral	1108:1114	The elemental Ca:Mg ratio in the mineral formed was similar to the Ca:Mg ratio of the ions added.
27869102	3	10	theme	microparticles	696:709	arg1	content					685:691	Mg content	682:691	Mg content of microparticles	682:709	It was hypothesized that increasing Mg content of microparticles would promote GG hydrogel formation.
27869102	16	11	contain	containing	1854:1863	arg1	microparticles					1839:1852	carbonate microparticles	1829:1852	carbonate microparticles containing a sufficient amount of Mg	1829:1889	In summary, carbonate microparticles containing a sufficient amount of Mg induced GG hydrogel formation, resulting in injectable, cytocompatible hydrogel-microparticle composites.
27869102	16	11	contain	containing	1854:1863	arg2	Mg					1888:1889	Mg	1888:1889	Mg	1888:1889	In summary, carbonate microparticles containing a sufficient amount of Mg induced GG hydrogel formation, resulting in injectable, cytocompatible hydrogel-microparticle composites.
27869102	16	11	contain	containing	1854:1863	arg2	amount					1878:1883	a sufficient amount	1865:1883	a sufficient amount of Mg	1865:1889	In summary, carbonate microparticles containing a sufficient amount of Mg induced GG hydrogel formation, resulting in injectable, cytocompatible hydrogel-microparticle composites.
27869102	10	12	theme	mineral	1329:1335	arg1	formation					1306:1314	formation	1306:1314	formation of amorphous mineral	1306:1335	Increasing the Mg content further caused formation of amorphous mineral.
27869102	1	13	theme	particle	285:292	arg1	form					294:297	particle form	285:297	particle form	285:297	The suitability of hydrogel biomaterials for bone regeneration can be improved by incorporation of an inorganic phase in particle form, thus maintaining hydrogel injectability.
27869102	11	14	theme	GG	1402:1403	arg1	formation					1414:1422	GG hydrogel formation	1402:1422	GG hydrogel formation	1402:1422	Microparticles of vaterite and magnesium calcite did not induce GG hydrogel formation, but addition of Mg-richer amorphous microparticles induced gelation within 20 min.
27869102	2	15	contain	containing	381:390	arg2	Mg					441:442	Mg	441:442	Mg	441:442	In this study, carbonate microparticles containing different amounts of calcium (Ca) and magnesium (Mg) were added to solutions of the anionic polysaccharide gellan gum (GG) to crosslink GG by release of Ca2+ and Mg2+ from microparticles and thereby induce formation of hydrogel-microparticle composites.
27869102	2	15	contain	containing	381:390	arg2	amounts					402:408	different amounts	392:408	different amounts of calcium (Ca) and magnesium (Mg)	392:443	In this study, carbonate microparticles containing different amounts of calcium (Ca) and magnesium (Mg) were added to solutions of the anionic polysaccharide gellan gum (GG) to crosslink GG by release of Ca2+ and Mg2+ from microparticles and thereby induce formation of hydrogel-microparticle composites.
27869102	2	15	contain	containing	381:390	arg2	calcium					413:419	calcium	413:419	calcium (Ca)	413:424	In this study, carbonate microparticles containing different amounts of calcium (Ca) and magnesium (Mg) were added to solutions of the anionic polysaccharide gellan gum (GG) to crosslink GG by release of Ca2+ and Mg2+ from microparticles and thereby induce formation of hydrogel-microparticle composites.
27869102	2	15	contain	containing	381:390	arg2	Ca					422:423	Ca	422:423	Ca	422:423	In this study, carbonate microparticles containing different amounts of calcium (Ca) and magnesium (Mg) were added to solutions of the anionic polysaccharide gellan gum (GG) to crosslink GG by release of Ca2+ and Mg2+ from microparticles and thereby induce formation of hydrogel-microparticle composites.
27869102	2	15	contain	containing	381:390	arg2	magnesium					430:438	magnesium	430:438	magnesium (Mg)	430:443	In this study, carbonate microparticles containing different amounts of calcium (Ca) and magnesium (Mg) were added to solutions of the anionic polysaccharide gellan gum (GG) to crosslink GG by release of Ca2+ and Mg2+ from microparticles and thereby induce formation of hydrogel-microparticle composites.
27869102	2	15	contain	containing	381:390	arg1	microparticles					366:379	carbonate microparticles	356:379	carbonate microparticles containing different amounts of calcium (Ca) and magnesium (Mg)	356:443	In this study, carbonate microparticles containing different amounts of calcium (Ca) and magnesium (Mg) were added to solutions of the anionic polysaccharide gellan gum (GG) to crosslink GG by release of Ca2+ and Mg2+ from microparticles and thereby induce formation of hydrogel-microparticle composites.
27869102	13	16	theme	hydrogel-microparticle	1623:1644	arg1	composites					1646:1655	hydrogel-microparticle composites	1623:1655	hydrogel-microparticle composites	1623:1655	MG-63 osteoblast-like cells were cultured in eluate from hydrogel-microparticle composites and on the composites themselves.
27869102	4	17	theme	incorporation	765:777	arg1	effect					752:757	The effect	748:757	The effect of Mg incorporation on cytocompatibility and cell growth	748:814	The effect of Mg incorporation on cytocompatibility and cell growth was also studied.
27869102	2	18	theme	composites	634:643	arg1	formation					598:606	formation	598:606	formation of hydrogel-microparticle composites	598:643	In this study, carbonate microparticles containing different amounts of calcium (Ca) and magnesium (Mg) were added to solutions of the anionic polysaccharide gellan gum (GG) to crosslink GG by release of Ca2+ and Mg2+ from microparticles and thereby induce formation of hydrogel-microparticle composites.
27869102	15	19	theme	equimolar	1794:1802	arg1	ratio					1810:1814	an equimolar Ca:Mg ratio	1791:1814	an equimolar Ca:Mg ratio	1791:1814	Cell growth was highest on composites containing particles with an equimolar Ca:Mg ratio.
27869102	1	20	theme	biomaterials	192:203	arg1	suitability					168:178	The suitability	164:178	The suitability of hydrogel biomaterials for bone regeneration	164:225	The suitability of hydrogel biomaterials for bone regeneration can be improved by incorporation of an inorganic phase in particle form, thus maintaining hydrogel injectability.
27869102	0	21	theme	calcium	116:122	arg1	microparticles					148:161	calcium and magnesium carbonate microparticles	116:161	calcium and magnesium carbonate microparticles	116:161	Novel injectable, self-gelling hydrogel-microparticle composites for bone regeneration consisting of gellan gum and calcium and magnesium carbonate microparticles.
27869102	3	22	theme	GG	725:726	arg1	formation					737:745	GG hydrogel formation	725:745	GG hydrogel formation	725:745	It was hypothesized that increasing Mg content of microparticles would promote GG hydrogel formation.
27869102	2	23	theme	hydrogel-microparticle	611:632	arg1	composites					634:643	hydrogel-microparticle composites	611:643	hydrogel-microparticle composites	611:643	In this study, carbonate microparticles containing different amounts of calcium (Ca) and magnesium (Mg) were added to solutions of the anionic polysaccharide gellan gum (GG) to crosslink GG by release of Ca2+ and Mg2+ from microparticles and thereby induce formation of hydrogel-microparticle composites.
27869102	7	24	theme	Mg	1145:1146	arg1	ratio					1148:1152	the Ca:Mg ratio	1138:1152	the Ca:Mg ratio of the ions added	1138:1170	The elemental Ca:Mg ratio in the mineral formed was similar to the Ca:Mg ratio of the ions added.
27869102	11	25	theme	hydrogel	1405:1412	arg1	formation					1414:1422	GG hydrogel formation	1402:1422	GG hydrogel formation	1402:1422	Microparticles of vaterite and magnesium calcite did not induce GG hydrogel formation, but addition of Mg-richer amorphous microparticles induced gelation within 20 min.
27869102	5	26	theme	Mg2+	880:883	arg1	ions					909:912	Ca2+ and Mg2+ and [Formula: see text] ions	871:912	Ca2+ and Mg2+ and [Formula: see text] ions	871:912	Microparticles were formed by mixing Ca2+ and Mg2+ and [Formula: see text] ions in varying concentrations.
27869102	0	27	theme	injectable	6:15	arg1	composites					54:63	Novel injectable, self-gelling hydrogel-microparticle composites	0:63	Novel injectable, self-gelling hydrogel-microparticle composites for bone regeneration consisting of gellan gum and calcium and magnesium carbonate microparticles.	0:162	Novel injectable, self-gelling hydrogel-microparticle composites for bone regeneration consisting of gellan gum and calcium and magnesium carbonate microparticles.
27869102	3	28	theme	hydrogel	728:735	arg1	formation					737:745	GG hydrogel formation	725:745	GG hydrogel formation	725:745	It was hypothesized that increasing Mg content of microparticles would promote GG hydrogel formation.
27869102	16	29	theme	hydrogel	1902:1909	arg1	formation					1911:1919	GG hydrogel formation	1899:1919	GG hydrogel formation	1899:1919	In summary, carbonate microparticles containing a sufficient amount of Mg induced GG hydrogel formation, resulting in injectable, cytocompatible hydrogel-microparticle composites.
27869102	1	30	theme	bone	209:212	arg1	regeneration					214:225	bone regeneration	209:225	bone regeneration	209:225	The suitability of hydrogel biomaterials for bone regeneration can be improved by incorporation of an inorganic phase in particle form, thus maintaining hydrogel injectability.
27869102	0	31	theme	Novel	0:4	arg1	composites					54:63	Novel injectable, self-gelling hydrogel-microparticle composites	0:63	Novel injectable, self-gelling hydrogel-microparticle composites for bone regeneration consisting of gellan gum and calcium and magnesium carbonate microparticles.	0:162	Novel injectable, self-gelling hydrogel-microparticle composites for bone regeneration consisting of gellan gum and calcium and magnesium carbonate microparticles.
27869102	4	32	from	effect	752:757	arg1	cytocompatibility					782:798	cytocompatibility	782:798	cytocompatibility	782:798	The effect of Mg incorporation on cytocompatibility and cell growth was also studied.
27869102	4	32	from	effect	752:757	arg1	growth					809:814	cell growth	804:814	cell growth	804:814	The effect of Mg incorporation on cytocompatibility and cell growth was also studied.
27869102	11	33	theme	Mg-richer	1441:1449	arg1	microparticles					1461:1474	Mg-richer amorphous microparticles	1441:1474	Mg-richer amorphous microparticles	1441:1474	Microparticles of vaterite and magnesium calcite did not induce GG hydrogel formation, but addition of Mg-richer amorphous microparticles induced gelation within 20 min.
27869102	2	34	theme	calcium	413:419	arg1	Ca					422:423	Ca	422:423	Ca	422:423	In this study, carbonate microparticles containing different amounts of calcium (Ca) and magnesium (Mg) were added to solutions of the anionic polysaccharide gellan gum (GG) to crosslink GG by release of Ca2+ and Mg2+ from microparticles and thereby induce formation of hydrogel-microparticle composites.
27869102	2	34	theme	calcium	413:419	arg1	magnesium					430:438	magnesium	430:438	magnesium (Mg)	430:443	In this study, carbonate microparticles containing different amounts of calcium (Ca) and magnesium (Mg) were added to solutions of the anionic polysaccharide gellan gum (GG) to crosslink GG by release of Ca2+ and Mg2+ from microparticles and thereby induce formation of hydrogel-microparticle composites.
27869102	2	34	theme	calcium	413:419	arg1	calcium					413:419	calcium	413:419	calcium (Ca)	413:424	In this study, carbonate microparticles containing different amounts of calcium (Ca) and magnesium (Mg) were added to solutions of the anionic polysaccharide gellan gum (GG) to crosslink GG by release of Ca2+ and Mg2+ from microparticles and thereby induce formation of hydrogel-microparticle composites.
27869102	2	34	theme	calcium	413:419	arg1	amounts					402:408	different amounts	392:408	different amounts of calcium (Ca) and magnesium (Mg)	392:443	In this study, carbonate microparticles containing different amounts of calcium (Ca) and magnesium (Mg) were added to solutions of the anionic polysaccharide gellan gum (GG) to crosslink GG by release of Ca2+ and Mg2+ from microparticles and thereby induce formation of hydrogel-microparticle composites.
27869102	2	34	theme	calcium	413:419	arg1	Mg					441:442	Mg	441:442	Mg	441:442	In this study, carbonate microparticles containing different amounts of calcium (Ca) and magnesium (Mg) were added to solutions of the anionic polysaccharide gellan gum (GG) to crosslink GG by release of Ca2+ and Mg2+ from microparticles and thereby induce formation of hydrogel-microparticle composites.
27869102	7	35	theme	ions	1161:1164	arg1	ratio					1148:1152	the Ca:Mg ratio	1138:1152	the Ca:Mg ratio of the ions added	1138:1170	The elemental Ca:Mg ratio in the mineral formed was similar to the Ca:Mg ratio of the ions added.
27869102	2	36	from	microparticles	564:577	arg1	release					534:540	release	534:540	release of Ca2+ and Mg2+ from microparticles	534:577	In this study, carbonate microparticles containing different amounts of calcium (Ca) and magnesium (Mg) were added to solutions of the anionic polysaccharide gellan gum (GG) to crosslink GG by release of Ca2+ and Mg2+ from microparticles and thereby induce formation of hydrogel-microparticle composites.
27869102	0	37	theme	carbonate	138:146	arg1	microparticles					148:161	calcium and magnesium carbonate microparticles	116:161	calcium and magnesium carbonate microparticles	116:161	Novel injectable, self-gelling hydrogel-microparticle composites for bone regeneration consisting of gellan gum and calcium and magnesium carbonate microparticles.
27869102	4	38	theme	Mg	762:763	arg1	incorporation					765:777	Mg incorporation	762:777	Mg incorporation	762:777	The effect of Mg incorporation on cytocompatibility and cell growth was also studied.
27869102	11	39	theme	amorphous	1451:1459	arg1	microparticles					1461:1474	Mg-richer amorphous microparticles	1441:1474	Mg-richer amorphous microparticles	1441:1474	Microparticles of vaterite and magnesium calcite did not induce GG hydrogel formation, but addition of Mg-richer amorphous microparticles induced gelation within 20 min.
27869102	7	40	theme	Mg	1092:1093	arg1	ratio					1095:1099	The elemental Ca:Mg ratio	1075:1099	The elemental Ca:Mg ratio in the mineral formed	1075:1121	The elemental Ca:Mg ratio in the mineral formed was similar to the Ca:Mg ratio of the ions added.
27869102	7	40	theme	Mg	1092:1093	arg1	similar					1127:1133	similar	1127:1133	similar	1127:1133	The elemental Ca:Mg ratio in the mineral formed was similar to the Ca:Mg ratio of the ions added.
27869102	0	41	theme	magnesium	128:136	arg1	microparticles					148:161	calcium and magnesium carbonate microparticles	116:161	calcium and magnesium carbonate microparticles	116:161	Novel injectable, self-gelling hydrogel-microparticle composites for bone regeneration consisting of gellan gum and calcium and magnesium carbonate microparticles.
27869102	16	42	theme	GG	1899:1900	arg1	formation					1911:1919	GG hydrogel formation	1899:1919	GG hydrogel formation	1899:1919	In summary, carbonate microparticles containing a sufficient amount of Mg induced GG hydrogel formation, resulting in injectable, cytocompatible hydrogel-microparticle composites.
27869102	10	43	theme	Mg	1280:1281	arg1	content					1283:1289	the Mg content	1276:1289	the Mg content	1276:1289	Increasing the Mg content further caused formation of amorphous mineral.
27869102	16	44	theme	injectable	1935:1944	arg1	composites					1985:1994	injectable, cytocompatible hydrogel-microparticle composites	1935:1994	injectable, cytocompatible hydrogel-microparticle composites	1935:1994	In summary, carbonate microparticles containing a sufficient amount of Mg induced GG hydrogel formation, resulting in injectable, cytocompatible hydrogel-microparticle composites.
27869102	2	45	theme	anionic	476:482	arg1	gum					506:508	the anionic polysaccharide gellan gum	472:508	the anionic polysaccharide gellan gum (GG)	472:513	In this study, carbonate microparticles containing different amounts of calcium (Ca) and magnesium (Mg) were added to solutions of the anionic polysaccharide gellan gum (GG) to crosslink GG by release of Ca2+ and Mg2+ from microparticles and thereby induce formation of hydrogel-microparticle composites.
27869102	2	45	theme	anionic	476:482	arg1	GG					511:512	GG	511:512	GG	511:512	In this study, carbonate microparticles containing different amounts of calcium (Ca) and magnesium (Mg) were added to solutions of the anionic polysaccharide gellan gum (GG) to crosslink GG by release of Ca2+ and Mg2+ from microparticles and thereby induce formation of hydrogel-microparticle composites.
27869102	1	46	theme	hydrogel	317:324	arg1	injectability					326:338	hydrogel injectability	317:338	hydrogel injectability	317:338	The suitability of hydrogel biomaterials for bone regeneration can be improved by incorporation of an inorganic phase in particle form, thus maintaining hydrogel injectability.
27869102	2	47	dep	crosslink	518:526	arg1	GG					528:529	GG	528:529	crosslink GG by release of Ca2+ and Mg2+ from microparticles	518:577	In this study, carbonate microparticles containing different amounts of calcium (Ca) and magnesium (Mg) were added to solutions of the anionic polysaccharide gellan gum (GG) to crosslink GG by release of Ca2+ and Mg2+ from microparticles and thereby induce formation of hydrogel-microparticle composites.
27869102	16	48	theme	hydrogel-microparticle	1962:1983	arg1	composites					1985:1994	injectable, cytocompatible hydrogel-microparticle composites	1935:1994	injectable, cytocompatible hydrogel-microparticle composites	1935:1994	In summary, carbonate microparticles containing a sufficient amount of Mg induced GG hydrogel formation, resulting in injectable, cytocompatible hydrogel-microparticle composites.
27869102	16	49	dep	injectable	1935:1944	arg1	cytocompatible					1947:1960	cytocompatible	1947:1960	cytocompatible	1947:1960	In summary, carbonate microparticles containing a sufficient amount of Mg induced GG hydrogel formation, resulting in injectable, cytocompatible hydrogel-microparticle composites.
27869102	2	50	theme	magnesium	430:438	arg1	Ca					422:423	Ca	422:423	Ca	422:423	In this study, carbonate microparticles containing different amounts of calcium (Ca) and magnesium (Mg) were added to solutions of the anionic polysaccharide gellan gum (GG) to crosslink GG by release of Ca2+ and Mg2+ from microparticles and thereby induce formation of hydrogel-microparticle composites.
27869102	2	50	theme	magnesium	430:438	arg1	magnesium					430:438	magnesium	430:438	magnesium (Mg)	430:443	In this study, carbonate microparticles containing different amounts of calcium (Ca) and magnesium (Mg) were added to solutions of the anionic polysaccharide gellan gum (GG) to crosslink GG by release of Ca2+ and Mg2+ from microparticles and thereby induce formation of hydrogel-microparticle composites.
27869102	2	50	theme	magnesium	430:438	arg1	calcium					413:419	calcium	413:419	calcium (Ca)	413:424	In this study, carbonate microparticles containing different amounts of calcium (Ca) and magnesium (Mg) were added to solutions of the anionic polysaccharide gellan gum (GG) to crosslink GG by release of Ca2+ and Mg2+ from microparticles and thereby induce formation of hydrogel-microparticle composites.
27869102	2	50	theme	magnesium	430:438	arg1	amounts					402:408	different amounts	392:408	different amounts of calcium (Ca) and magnesium (Mg)	392:443	In this study, carbonate microparticles containing different amounts of calcium (Ca) and magnesium (Mg) were added to solutions of the anionic polysaccharide gellan gum (GG) to crosslink GG by release of Ca2+ and Mg2+ from microparticles and thereby induce formation of hydrogel-microparticle composites.
27869102	2	50	theme	magnesium	430:438	arg1	Mg					441:442	Mg	441:442	Mg	441:442	In this study, carbonate microparticles containing different amounts of calcium (Ca) and magnesium (Mg) were added to solutions of the anionic polysaccharide gellan gum (GG) to crosslink GG by release of Ca2+ and Mg2+ from microparticles and thereby induce formation of hydrogel-microparticle composites.
27869102	0	51	theme	hydrogel-microparticle	31:52	arg1	composites					54:63	Novel injectable, self-gelling hydrogel-microparticle composites	0:63	Novel injectable, self-gelling hydrogel-microparticle composites for bone regeneration consisting of gellan gum and calcium and magnesium carbonate microparticles.	0:162	Novel injectable, self-gelling hydrogel-microparticle composites for bone regeneration consisting of gellan gum and calcium and magnesium carbonate microparticles.
27869102	11	52	theme	vaterite	1356:1363	arg1	Microparticles					1338:1351	Microparticles	1338:1351	Microparticles of vaterite and magnesium calcite	1338:1385	Microparticles of vaterite and magnesium calcite did not induce GG hydrogel formation, but addition of Mg-richer amorphous microparticles induced gelation within 20 min.
27869102	9	53	theme	low	1219:1221	arg1	content					1226:1232	low Mg content	1219:1232	low Mg content	1219:1232	At low Mg content, magnesian calcite was formed.
27869102	8	54	theme	Mg	1191:1192	arg1	absence					1180:1186	the absence	1176:1186	the absence of Mg	1176:1192	In the absence of Mg, vaterite was formed.
27869102	5	55	theme	Ca2+	871:874	arg1	ions					909:912	Ca2+ and Mg2+ and [Formula: see text] ions	871:912	Ca2+ and Mg2+ and [Formula: see text] ions	871:912	Microparticles were formed by mixing Ca2+ and Mg2+ and [Formula: see text] ions in varying concentrations.
27869102	0	56	theme	bone	69:72	arg1	regeneration					74:85	bone regeneration	69:85	bone regeneration consisting of gellan gum and calcium and magnesium carbonate microparticles	69:161	Novel injectable, self-gelling hydrogel-microparticle composites for bone regeneration consisting of gellan gum and calcium and magnesium carbonate microparticles.
27869102	16	57	theme	carbonate	1829:1837	arg1	microparticles					1839:1852	carbonate microparticles	1829:1852	carbonate microparticles containing a sufficient amount of Mg	1829:1889	In summary, carbonate microparticles containing a sufficient amount of Mg induced GG hydrogel formation, resulting in injectable, cytocompatible hydrogel-microparticle composites.
27869102	7	58	theme	Ca	1089:1090	arg1	ratio					1095:1099	The elemental Ca:Mg ratio	1075:1099	The elemental Ca:Mg ratio in the mineral formed	1075:1121	The elemental Ca:Mg ratio in the mineral formed was similar to the Ca:Mg ratio of the ions added.
27869102	7	58	theme	Ca	1089:1090	arg1	similar					1127:1133	similar	1127:1133	similar	1127:1133	The elemental Ca:Mg ratio in the mineral formed was similar to the Ca:Mg ratio of the ions added.
27869102	9	59	theme	Mg	1223:1224	arg1	content					1226:1232	low Mg content	1219:1232	low Mg content	1219:1232	At low Mg content, magnesian calcite was formed.
27869102	13	60	theme	osteoblast-like	1572:1586	arg1	cells					1588:1592	MG-63 osteoblast-like cells	1566:1592	MG-63 osteoblast-like cells	1566:1592	MG-63 osteoblast-like cells were cultured in eluate from hydrogel-microparticle composites and on the composites themselves.
27869102	16	61	theme	sufficient	1867:1876	arg1	amount					1878:1883	a sufficient amount	1865:1883	a sufficient amount of Mg	1865:1889	In summary, carbonate microparticles containing a sufficient amount of Mg induced GG hydrogel formation, resulting in injectable, cytocompatible hydrogel-microparticle composites.
27869102	16	61	theme	sufficient	1867:1876	arg1	Mg					1888:1889	Mg	1888:1889	Mg	1888:1889	In summary, carbonate microparticles containing a sufficient amount of Mg induced GG hydrogel formation, resulting in injectable, cytocompatible hydrogel-microparticle composites.
27869102	2	62	theme	Mg2+	554:557	arg1	release					534:540	release	534:540	release of Ca2+ and Mg2+ from microparticles	534:577	In this study, carbonate microparticles containing different amounts of calcium (Ca) and magnesium (Mg) were added to solutions of the anionic polysaccharide gellan gum (GG) to crosslink GG by release of Ca2+ and Mg2+ from microparticles and thereby induce formation of hydrogel-microparticle composites.
27869102	3	63	theme	Mg	682:683	arg1	content					685:691	Mg content	682:691	Mg content of microparticles	682:709	It was hypothesized that increasing Mg content of microparticles would promote GG hydrogel formation.
27869102	5	64	dep	Mg2+	880:883	arg1	see					899:901	see	899:901	see text	899:906	Microparticles were formed by mixing Ca2+ and Mg2+ and [Formula: see text] ions in varying concentrations.
27869102	2	65	theme	carbonate	356:364	arg1	microparticles					366:379	carbonate microparticles	356:379	carbonate microparticles containing different amounts of calcium (Ca) and magnesium (Mg)	356:443	In this study, carbonate microparticles containing different amounts of calcium (Ca) and magnesium (Mg) were added to solutions of the anionic polysaccharide gellan gum (GG) to crosslink GG by release of Ca2+ and Mg2+ from microparticles and thereby induce formation of hydrogel-microparticle composites.
27869102	11	66	dep	vaterite	1356:1363	arg1	calcite					1379:1385	calcite	1379:1385	calcite	1379:1385	Microparticles of vaterite and magnesium calcite did not induce GG hydrogel formation, but addition of Mg-richer amorphous microparticles induced gelation within 20 min.
27869102	1	67	from	incorporation	246:258	arg1	form					294:297	particle form	285:297	particle form	285:297	The suitability of hydrogel biomaterials for bone regeneration can be improved by incorporation of an inorganic phase in particle form, thus maintaining hydrogel injectability.
27869102	15	68	theme	Cell	1727:1730	arg1	growth					1732:1737	Cell growth	1727:1737	Cell growth	1727:1737	Cell growth was highest on composites containing particles with an equimolar Ca:Mg ratio.
27869102	13	69	theme	MG-63	1566:1570	arg1	cells					1588:1592	MG-63 osteoblast-like cells	1566:1592	MG-63 osteoblast-like cells	1566:1592	MG-63 osteoblast-like cells were cultured in eluate from hydrogel-microparticle composites and on the composites themselves.
27869102	5	70	theme	varying	917:923	arg1	concentrations					925:938	varying concentrations	917:938	varying concentrations	917:938	Microparticles were formed by mixing Ca2+ and Mg2+ and [Formula: see text] ions in varying concentrations.
27869102	15	71	theme	Mg	1807:1808	arg1	ratio					1810:1814	an equimolar Ca:Mg ratio	1791:1814	an equimolar Ca:Mg ratio	1791:1814	Cell growth was highest on composites containing particles with an equimolar Ca:Mg ratio.
27869102	6	72	theme	GG	1020:1021	arg1	solution					1023:1030	GG solution	1020:1030	GG solution	1020:1030	Microparticles were characterized physiochemically and subsequently mixed with GG solution to form hydrogel-microparticle composites.
27869102	2	73	theme	Ca2+	545:548	arg1	release					534:540	release	534:540	release of Ca2+ and Mg2+ from microparticles	534:577	In this study, carbonate microparticles containing different amounts of calcium (Ca) and magnesium (Mg) were added to solutions of the anionic polysaccharide gellan gum (GG) to crosslink GG by release of Ca2+ and Mg2+ from microparticles and thereby induce formation of hydrogel-microparticle composites.
27869102	9	74	theme	magnesian	1235:1243	arg1	calcite					1245:1251	magnesian calcite	1235:1251	magnesian calcite	1235:1251	At low Mg content, magnesian calcite was formed.
27869102	4	75	theme	cell	804:807	arg1	growth					809:814	cell growth	804:814	cell growth	804:814	The effect of Mg incorporation on cytocompatibility and cell growth was also studied.
27869102	11	76	theme	microparticles	1461:1474	arg1	addition					1429:1436	addition	1429:1436	addition of Mg-richer amorphous microparticles	1429:1474	Microparticles of vaterite and magnesium calcite did not induce GG hydrogel formation, but addition of Mg-richer amorphous microparticles induced gelation within 20 min.
27869102	11	77	theme	magnesium	1369:1377	arg1	Microparticles					1338:1351	Microparticles	1338:1351	Microparticles of vaterite and magnesium calcite	1338:1385	Microparticles of vaterite and magnesium calcite did not induce GG hydrogel formation, but addition of Mg-richer amorphous microparticles induced gelation within 20 min.
27869102	0	78	dep	injectable	6:15	arg1	self-gelling					18:29	self-gelling	18:29	self-gelling	18:29	Novel injectable, self-gelling hydrogel-microparticle composites for bone regeneration consisting of gellan gum and calcium and magnesium carbonate microparticles.
27869102	15	79	theme	Ca	1804:1805	arg1	ratio					1810:1814	an equimolar Ca:Mg ratio	1791:1814	an equimolar Ca:Mg ratio	1791:1814	Cell growth was highest on composites containing particles with an equimolar Ca:Mg ratio.
27869102	6	80	theme	hydrogel-microparticle	1040:1061	arg1	composites					1063:1072	hydrogel-microparticle composites	1040:1072	hydrogel-microparticle composites	1040:1072	Microparticles were characterized physiochemically and subsequently mixed with GG solution to form hydrogel-microparticle composites.
27869102	2	81	theme	gum	506:508	arg1	solutions					459:467	solutions	459:467	solutions of the anionic polysaccharide gellan gum (GG)	459:513	In this study, carbonate microparticles containing different amounts of calcium (Ca) and magnesium (Mg) were added to solutions of the anionic polysaccharide gellan gum (GG) to crosslink GG by release of Ca2+ and Mg2+ from microparticles and thereby induce formation of hydrogel-microparticle composites.
27869102	16	82	theme	Mg	1888:1889	arg1	amount					1878:1883	a sufficient amount	1865:1883	a sufficient amount of Mg	1865:1889	In summary, carbonate microparticles containing a sufficient amount of Mg induced GG hydrogel formation, resulting in injectable, cytocompatible hydrogel-microparticle composites.
27869102	16	82	theme	Mg	1888:1889	arg1	Mg					1888:1889	Mg	1888:1889	Mg	1888:1889	In summary, carbonate microparticles containing a sufficient amount of Mg induced GG hydrogel formation, resulting in injectable, cytocompatible hydrogel-microparticle composites.
27869102	1	83	theme	inorganic	266:274	arg1	phase					276:280	an inorganic phase	263:280	an inorganic phase	263:280	The suitability of hydrogel biomaterials for bone regeneration can be improved by incorporation of an inorganic phase in particle form, thus maintaining hydrogel injectability.
25046106	9	0	from	abundance	1799:1807	arg1	feces					1766:1770	feces	1766:1770	feces	1766:1770	Whereas pigs fed diet WB had about 2 to 22 times higher abundance (P<0.05) of Megasphaera elsdenii in feces and about six times higher abundance (P<0.05) of Lactobacillus reuteri in ileal digesta than pigs fed the other diets.
25046106	9	0	from	abundance	1799:1807	arg1	digesta					1852:1858	ileal digesta	1846:1858	ileal digesta	1846:1858	Whereas pigs fed diet WB had about 2 to 22 times higher abundance (P<0.05) of Megasphaera elsdenii in feces and about six times higher abundance (P<0.05) of Lactobacillus reuteri in ileal digesta than pigs fed the other diets.
25046106	2	1	theme	fermentation	364:375	arg1	products					377:384	its fermentation products	360:384	its fermentation products	360:384	The knowledge about effects of these sources of NSP on the gut microbiota and its fermentation products is still scarce.
25046106	9	2	dep	22	1704:1705	arg1	to					1701:1702	to	1701:1702	to	1701:1702	Whereas pigs fed diet WB had about 2 to 22 times higher abundance (P<0.05) of Megasphaera elsdenii in feces and about six times higher abundance (P<0.05) of Lactobacillus reuteri in ileal digesta than pigs fed the other diets.
25046106	8	3	theme	23	1605:1606	arg1	times					1608:1612	times	1608:1612	times	1608:1612	In pigs fed diet CFO, three TRFs related to Prevotellaceae together consisted of more than 25% of the fecal microbiota, which is about 3 to 23 times higher (P<0.05) than in pigs fed the other diets.
25046106	8	3	theme	23	1605:1606	arg1	microbiota					1573:1582	the fecal microbiota	1563:1582	the fecal microbiota	1563:1582	In pigs fed diet CFO, three TRFs related to Prevotellaceae together consisted of more than 25% of the fecal microbiota, which is about 3 to 23 times higher (P<0.05) than in pigs fed the other diets.
25046106	8	4	theme	microbiota	1573:1582	arg1	%					1558:1558	more than 25%	1546:1558	more than 25% of the fecal microbiota, which is about 3 to 23 times higher (P<0.05) than in pigs fed the other diets	1546:1661	In pigs fed diet CFO, three TRFs related to Prevotellaceae together consisted of more than 25% of the fecal microbiota, which is about 3 to 23 times higher (P<0.05) than in pigs fed the other diets.
25046106	8	4	theme	microbiota	1573:1582	arg1	microbiota					1573:1582	the fecal microbiota	1563:1582	the fecal microbiota	1563:1582	In pigs fed diet CFO, three TRFs related to Prevotellaceae together consisted of more than 25% of the fecal microbiota, which is about 3 to 23 times higher (P<0.05) than in pigs fed the other diets.
25046106	8	4	theme	microbiota	1573:1582	arg1	times					1608:1612	times	1608:1612	times	1608:1612	In pigs fed diet CFO, three TRFs related to Prevotellaceae together consisted of more than 25% of the fecal microbiota, which is about 3 to 23 times higher (P<0.05) than in pigs fed the other diets.
25046106	6	5	theme	microbial	1057:1065	arg1	composition					1067:1077	The gut microbial composition	1049:1077	The gut microbial composition	1049:1077	The gut microbial composition was assessed with terminal restriction fragment (TRF) length polymorphism and the abundance of Lactobacillus spp., Enterobacteriaceae, Bacteroides-Prevotella-Porphyromonas and the β-xylosidase gene, xynB, were assessed with quantitative PCR.
25046106	9	6	theme	higher	1713:1718	arg1	abundance					1720:1728	about 2 to 22 times higher abundance	1693:1728	about 2 to 22 times higher abundance (P<0.05) of Megasphaera elsdenii in feces	1693:1770	Whereas pigs fed diet WB had about 2 to 22 times higher abundance (P<0.05) of Megasphaera elsdenii in feces and about six times higher abundance (P<0.05) of Lactobacillus reuteri in ileal digesta than pigs fed the other diets.
25046106	9	6	theme	higher	1713:1718	arg1	P<0.05					1731:1736	P<0.05	1731:1736	P<0.05	1731:1736	Whereas pigs fed diet WB had about 2 to 22 times higher abundance (P<0.05) of Megasphaera elsdenii in feces and about six times higher abundance (P<0.05) of Lactobacillus reuteri in ileal digesta than pigs fed the other diets.
25046106	7	7	theme	NSP	1365:1367	arg1	structure					1369:1377	NSP structure	1365:1377	NSP structure (arabinoxylan or pectin)	1365:1402	The gut microbiota did not cluster based on NSP structure (arabinoxylan or pectin) rather, the effect was to a high degree ingredient specific.
25046106	7	8	theme	gut	1325:1327	arg1	microbiota					1329:1338	The gut microbiota	1321:1338	The gut microbiota	1321:1338	The gut microbiota did not cluster based on NSP structure (arabinoxylan or pectin) rather, the effect was to a high degree ingredient specific.
25046106	8	9	from	times	1608:1612	arg1	pigs					1638:1641	pigs	1638:1641	pigs fed the other diets	1638:1661	In pigs fed diet CFO, three TRFs related to Prevotellaceae together consisted of more than 25% of the fecal microbiota, which is about 3 to 23 times higher (P<0.05) than in pigs fed the other diets.
25046106	6	10	theme	length	1133:1138	arg1	polymorphism					1140:1151	terminal restriction fragment (TRF) length polymorphism	1097:1151	terminal restriction fragment (TRF) length polymorphism	1097:1151	The gut microbial composition was assessed with terminal restriction fragment (TRF) length polymorphism and the abundance of Lactobacillus spp., Enterobacteriaceae, Bacteroides-Prevotella-Porphyromonas and the β-xylosidase gene, xynB, were assessed with quantitative PCR.
25046106	9	11	dep	times	1786:1790	arg1	higher					1792:1797	higher	1792:1797	higher	1792:1797	Whereas pigs fed diet WB had about 2 to 22 times higher abundance (P<0.05) of Megasphaera elsdenii in feces and about six times higher abundance (P<0.05) of Lactobacillus reuteri in ileal digesta than pigs fed the other diets.
25046106	12	12	from	proportion	2350:2359	arg1	feces					2387:2391	feces	2387:2391	feces	2387:2391	Moreover, chicory pectin increased the acetic acid proportion in both ileal digesta and feces.
25046106	12	12	from	proportion	2350:2359	arg1	digesta					2375:2381	ileal digesta	2369:2381	ileal digesta	2369:2381	Moreover, chicory pectin increased the acetic acid proportion in both ileal digesta and feces.
25046106	10	13	theme	fiber	1980:1984	arg1	fiber					1980:1984	dietary fiber	1972:1984	dietary fiber (r=0.60; P=0.001)	1972:2002	The total amount of digested NSP (r=0.57; P=0.002), xylose (r=0.53; P=0.004) and dietary fiber (r=0.60; P=0.001) in ileal digesta were positively correlated with an increased abundance of Bacteroides-Prevotella-Porphyromonas.
25046106	10	13	theme	fiber	1980:1984	arg1	amount					1901:1906	The total amount	1891:1906	The total amount of digested NSP (r=0.57; P=0.002), xylose (r=0.53; P=0.004) and dietary fiber (r=0.60; P=0.001) in ileal digesta	1891:2019	The total amount of digested NSP (r=0.57; P=0.002), xylose (r=0.53; P=0.004) and dietary fiber (r=0.60; P=0.001) in ileal digesta were positively correlated with an increased abundance of Bacteroides-Prevotella-Porphyromonas.
25046106	10	13	theme	fiber	1980:1984	arg1	NSP					1920:1922	digested NSP	1911:1922	digested NSP (r=0.57; P=0.002)	1911:1940	The total amount of digested NSP (r=0.57; P=0.002), xylose (r=0.53; P=0.004) and dietary fiber (r=0.60; P=0.001) in ileal digesta were positively correlated with an increased abundance of Bacteroides-Prevotella-Porphyromonas.
25046106	10	13	theme	fiber	1980:1984	arg1	xylose					1943:1948	xylose	1943:1948	xylose (r=0.53; P=0.004)	1943:1966	The total amount of digested NSP (r=0.57; P=0.002), xylose (r=0.53; P=0.004) and dietary fiber (r=0.60; P=0.001) in ileal digesta were positively correlated with an increased abundance of Bacteroides-Prevotella-Porphyromonas.
25046106	10	14	theme	increased	2056:2064	arg1	abundance					2066:2074	an increased abundance	2053:2074	an increased abundance of Bacteroides-Prevotella-Porphyromonas	2053:2114	The total amount of digested NSP (r=0.57; P=0.002), xylose (r=0.53; P=0.004) and dietary fiber (r=0.60; P=0.001) in ileal digesta were positively correlated with an increased abundance of Bacteroides-Prevotella-Porphyromonas.
25046106	10	15	theme	NSP	1920:1922	arg1	fiber					1980:1984	dietary fiber	1972:1984	dietary fiber (r=0.60; P=0.001)	1972:2002	The total amount of digested NSP (r=0.57; P=0.002), xylose (r=0.53; P=0.004) and dietary fiber (r=0.60; P=0.001) in ileal digesta were positively correlated with an increased abundance of Bacteroides-Prevotella-Porphyromonas.
25046106	10	15	theme	NSP	1920:1922	arg1	amount					1901:1906	The total amount	1891:1906	The total amount of digested NSP (r=0.57; P=0.002), xylose (r=0.53; P=0.004) and dietary fiber (r=0.60; P=0.001) in ileal digesta	1891:2019	The total amount of digested NSP (r=0.57; P=0.002), xylose (r=0.53; P=0.004) and dietary fiber (r=0.60; P=0.001) in ileal digesta were positively correlated with an increased abundance of Bacteroides-Prevotella-Porphyromonas.
25046106	10	15	theme	NSP	1920:1922	arg1	NSP					1920:1922	digested NSP	1911:1922	digested NSP (r=0.57; P=0.002)	1911:1940	The total amount of digested NSP (r=0.57; P=0.002), xylose (r=0.53; P=0.004) and dietary fiber (r=0.60; P=0.001) in ileal digesta were positively correlated with an increased abundance of Bacteroides-Prevotella-Porphyromonas.
25046106	10	15	theme	NSP	1920:1922	arg1	xylose					1943:1948	xylose	1943:1948	xylose (r=0.53; P=0.004)	1943:1966	The total amount of digested NSP (r=0.57; P=0.002), xylose (r=0.53; P=0.004) and dietary fiber (r=0.60; P=0.001) in ileal digesta were positively correlated with an increased abundance of Bacteroides-Prevotella-Porphyromonas.
25046106	6	16	theme	β-xylosidase	1259:1270	arg1	xynB					1278:1281	xynB	1278:1281	xynB	1278:1281	The gut microbial composition was assessed with terminal restriction fragment (TRF) length polymorphism and the abundance of Lactobacillus spp., Enterobacteriaceae, Bacteroides-Prevotella-Porphyromonas and the β-xylosidase gene, xynB, were assessed with quantitative PCR.
25046106	6	16	theme	β-xylosidase	1259:1270	arg1	gene					1272:1275	the β-xylosidase gene	1255:1275	the β-xylosidase gene	1255:1275	The gut microbial composition was assessed with terminal restriction fragment (TRF) length polymorphism and the abundance of Lactobacillus spp., Enterobacteriaceae, Bacteroides-Prevotella-Porphyromonas and the β-xylosidase gene, xynB, were assessed with quantitative PCR.
25046106	3	17	theme	ileal	503:507	arg1	composition					529:539	the ileal and fecal microbial composition	499:539	the ileal and fecal microbial composition	499:539	The aim of this study was to investigate effects of feeding diets with native sources of NSP on the ileal and fecal microbial composition and the dietary impact on the concentration of short-chain fatty acids (SCFA) and lactic acid.
25046106	9	18	theme	Megasphaera	1742:1752	arg1	abundance					1720:1728	about 2 to 22 times higher abundance	1693:1728	about 2 to 22 times higher abundance (P<0.05) of Megasphaera elsdenii in feces	1693:1770	Whereas pigs fed diet WB had about 2 to 22 times higher abundance (P<0.05) of Megasphaera elsdenii in feces and about six times higher abundance (P<0.05) of Lactobacillus reuteri in ileal digesta than pigs fed the other diets.
25046106	9	18	theme	Megasphaera	1742:1752	arg1	P<0.05					1810:1815	P<0.05	1810:1815	P<0.05	1810:1815	Whereas pigs fed diet WB had about 2 to 22 times higher abundance (P<0.05) of Megasphaera elsdenii in feces and about six times higher abundance (P<0.05) of Lactobacillus reuteri in ileal digesta than pigs fed the other diets.
25046106	9	18	theme	Megasphaera	1742:1752	arg1	abundance					1799:1807	about six times higher abundance	1776:1807	about six times higher abundance (P<0.05)	1776:1816	Whereas pigs fed diet WB had about 2 to 22 times higher abundance (P<0.05) of Megasphaera elsdenii in feces and about six times higher abundance (P<0.05) of Lactobacillus reuteri in ileal digesta than pigs fed the other diets.
25046106	9	18	theme	Megasphaera	1742:1752	arg1	P<0.05					1731:1736	P<0.05	1731:1736	P<0.05	1731:1736	Whereas pigs fed diet WB had about 2 to 22 times higher abundance (P<0.05) of Megasphaera elsdenii in feces and about six times higher abundance (P<0.05) of Lactobacillus reuteri in ileal digesta than pigs fed the other diets.
25046106	3	19	theme	fatty	600:604	arg1	SCFA					613:616	SCFA	613:616	SCFA	613:616	The aim of this study was to investigate effects of feeding diets with native sources of NSP on the ileal and fecal microbial composition and the dietary impact on the concentration of short-chain fatty acids (SCFA) and lactic acid.
25046106	3	19	theme	fatty	600:604	arg1	acids					606:610	short-chain fatty acids	588:610	short-chain fatty acids (SCFA)	588:617	The aim of this study was to investigate effects of feeding diets with native sources of NSP on the ileal and fecal microbial composition and the dietary impact on the concentration of short-chain fatty acids (SCFA) and lactic acid.
25046106	1	20	theme	non-starch	248:257	arg1	NSP					276:278	NSP	276:278	NSP	276:278	Most plant-origin fiber sources used in pig production contains a mixture of soluble and insoluble non-starch polysaccharides (NSP).
25046106	1	20	theme	non-starch	248:257	arg1	polysaccharides					259:273	soluble and insoluble non-starch polysaccharides	226:273	soluble and insoluble non-starch polysaccharides (NSP)	226:279	Most plant-origin fiber sources used in pig production contains a mixture of soluble and insoluble non-starch polysaccharides (NSP).
25046106	11	21	theme	acid	2282:2285	arg1	proportion					2287:2296	the fecal butyric acid proportion	2264:2296	the fecal butyric acid proportion	2264:2296	The effect on SCFA was correlated to specific neutral sugars where xylose increased the ileal butyric acid proportion, whereas arabinose increased the fecal butyric acid proportion.
25046106	5	22	dep	chicory	919:925	arg1	pulp					955:958	pulp	955:958	pulp	955:958	The four diets were balanced to be similar in NSP content and included one of four fiber sources, two diets were rich in pectins, through inclusion of chicory forage (CFO) and sugar beet pulp, and two were rich in arabinoxylan, through inclusion of wheat bran (WB) and grass meal.
25046106	5	22	dep	chicory	919:925	arg1	forage					927:932	forage	927:932	forage	927:932	The four diets were balanced to be similar in NSP content and included one of four fiber sources, two diets were rich in pectins, through inclusion of chicory forage (CFO) and sugar beet pulp, and two were rich in arabinoxylan, through inclusion of wheat bran (WB) and grass meal.
25046106	12	23	theme	acid	2345:2348	arg1	proportion					2350:2359	the acetic acid proportion	2334:2359	the acetic acid proportion in both ileal digesta and feces	2334:2391	Moreover, chicory pectin increased the acetic acid proportion in both ileal digesta and feces.
25046106	5	24	from	similar	803:809	arg1	content					818:824	NSP content	814:824	NSP content	814:824	The four diets were balanced to be similar in NSP content and included one of four fiber sources, two diets were rich in pectins, through inclusion of chicory forage (CFO) and sugar beet pulp, and two were rich in arabinoxylan, through inclusion of wheat bran (WB) and grass meal.
25046106	1	25	theme	plant-origin	154:165	arg1	sources					173:179	Most plant-origin fiber sources	149:179	Most plant-origin fiber sources used in pig production	149:202	Most plant-origin fiber sources used in pig production contains a mixture of soluble and insoluble non-starch polysaccharides (NSP).
25046106	10	26	dep	fiber	1980:1984	arg1	r=0.60					1987:1992	r=0.60	1987:1992	r=0.60	1987:1992	The total amount of digested NSP (r=0.57; P=0.002), xylose (r=0.53; P=0.004) and dietary fiber (r=0.60; P=0.001) in ileal digesta were positively correlated with an increased abundance of Bacteroides-Prevotella-Porphyromonas.
25046106	12	27	theme	acetic	2338:2343	arg1	proportion					2350:2359	the acetic acid proportion	2334:2359	the acetic acid proportion in both ileal digesta and feces	2334:2391	Moreover, chicory pectin increased the acetic acid proportion in both ileal digesta and feces.
25046106	9	28	from	abundance	1720:1728	arg1	feces					1766:1770	feces	1766:1770	feces	1766:1770	Whereas pigs fed diet WB had about 2 to 22 times higher abundance (P<0.05) of Megasphaera elsdenii in feces and about six times higher abundance (P<0.05) of Lactobacillus reuteri in ileal digesta than pigs fed the other diets.
25046106	9	28	from	abundance	1720:1728	arg1	digesta					1852:1858	ileal digesta	1846:1858	ileal digesta	1846:1858	Whereas pigs fed diet WB had about 2 to 22 times higher abundance (P<0.05) of Megasphaera elsdenii in feces and about six times higher abundance (P<0.05) of Lactobacillus reuteri in ileal digesta than pigs fed the other diets.
25046106	6	29	theme	restriction	1106:1116	arg1	polymorphism					1140:1151	terminal restriction fragment (TRF) length polymorphism	1097:1151	terminal restriction fragment (TRF) length polymorphism	1097:1151	The gut microbial composition was assessed with terminal restriction fragment (TRF) length polymorphism and the abundance of Lactobacillus spp., Enterobacteriaceae, Bacteroides-Prevotella-Porphyromonas and the β-xylosidase gene, xynB, were assessed with quantitative PCR.
25046106	3	30	from	impact	557:562	arg1	concentration					571:583	the concentration	567:583	the concentration of short-chain fatty acids (SCFA) and lactic acid	567:633	The aim of this study was to investigate effects of feeding diets with native sources of NSP on the ileal and fecal microbial composition and the dietary impact on the concentration of short-chain fatty acids (SCFA) and lactic acid.
25046106	3	30	from	impact	557:562	arg1	composition					529:539	the ileal and fecal microbial composition	499:539	the ileal and fecal microbial composition	499:539	The aim of this study was to investigate effects of feeding diets with native sources of NSP on the ileal and fecal microbial composition and the dietary impact on the concentration of short-chain fatty acids (SCFA) and lactic acid.
25046106	10	31	dep	r=0.60	1987:1992	arg1	P=0.001					1995:2001	P=0.001	1995:2001	r=0.60; P=0.001	1987:2001	The total amount of digested NSP (r=0.57; P=0.002), xylose (r=0.53; P=0.004) and dietary fiber (r=0.60; P=0.001) in ileal digesta were positively correlated with an increased abundance of Bacteroides-Prevotella-Porphyromonas.
25046106	9	32	theme	six	1782:1784	arg1	times					1786:1790	times	1786:1790	times	1786:1790	Whereas pigs fed diet WB had about 2 to 22 times higher abundance (P<0.05) of Megasphaera elsdenii in feces and about six times higher abundance (P<0.05) of Lactobacillus reuteri in ileal digesta than pigs fed the other diets.
25046106	2	33	from	effects	302:308	arg1	products					377:384	its fermentation products	360:384	its fermentation products	360:384	The knowledge about effects of these sources of NSP on the gut microbiota and its fermentation products is still scarce.
25046106	2	33	from	effects	302:308	arg1	microbiota					345:354	the gut microbiota	337:354	the gut microbiota	337:354	The knowledge about effects of these sources of NSP on the gut microbiota and its fermentation products is still scarce.
25046106	6	34	theme	quantitative	1303:1314	arg1	PCR					1316:1318	quantitative PCR	1303:1318	quantitative PCR	1303:1318	The gut microbial composition was assessed with terminal restriction fragment (TRF) length polymorphism and the abundance of Lactobacillus spp., Enterobacteriaceae, Bacteroides-Prevotella-Porphyromonas and the β-xylosidase gene, xynB, were assessed with quantitative PCR.
25046106	11	35	from	effect	2121:2126	arg1	SCFA					2131:2134	SCFA	2131:2134	SCFA	2131:2134	The effect on SCFA was correlated to specific neutral sugars where xylose increased the ileal butyric acid proportion, whereas arabinose increased the fecal butyric acid proportion.
25046106	5	36	theme	fiber	851:855	arg1	sources					857:863	four fiber sources	846:863	four fiber sources	846:863	The four diets were balanced to be similar in NSP content and included one of four fiber sources, two diets were rich in pectins, through inclusion of chicory forage (CFO) and sugar beet pulp, and two were rich in arabinoxylan, through inclusion of wheat bran (WB) and grass meal.
25046106	0	37	theme	microbial	112:120	arg1	community					122:130	ileal microbial community	106:130	ileal microbial community of growing pigs	106:146	Fermentable non-starch polysaccharides increases the abundance of Bacteroides-Prevotella-Porphyromonas in ileal microbial community of growing pigs.
25046106	9	38	contain	had	1689:1691	arg2	abundance					1799:1807	about six times higher abundance	1776:1807	about six times higher abundance (P<0.05)	1776:1816	Whereas pigs fed diet WB had about 2 to 22 times higher abundance (P<0.05) of Megasphaera elsdenii in feces and about six times higher abundance (P<0.05) of Lactobacillus reuteri in ileal digesta than pigs fed the other diets.
25046106	9	38	contain	had	1689:1691	arg2	abundance					1720:1728	about 2 to 22 times higher abundance	1693:1728	about 2 to 22 times higher abundance (P<0.05) of Megasphaera elsdenii in feces	1693:1770	Whereas pigs fed diet WB had about 2 to 22 times higher abundance (P<0.05) of Megasphaera elsdenii in feces and about six times higher abundance (P<0.05) of Lactobacillus reuteri in ileal digesta than pigs fed the other diets.
25046106	9	38	contain	had	1689:1691	arg2	P<0.05					1810:1815	P<0.05	1810:1815	P<0.05	1810:1815	Whereas pigs fed diet WB had about 2 to 22 times higher abundance (P<0.05) of Megasphaera elsdenii in feces and about six times higher abundance (P<0.05) of Lactobacillus reuteri in ileal digesta than pigs fed the other diets.
25046106	9	38	contain	had	1689:1691	arg2	P<0.05					1731:1736	P<0.05	1731:1736	P<0.05	1731:1736	Whereas pigs fed diet WB had about 2 to 22 times higher abundance (P<0.05) of Megasphaera elsdenii in feces and about six times higher abundance (P<0.05) of Lactobacillus reuteri in ileal digesta than pigs fed the other diets.
25046106	9	38	contain	had	1689:1691	arg1	pigs					1672:1675	pigs	1672:1675	pigs fed diet WB	1672:1687	Whereas pigs fed diet WB had about 2 to 22 times higher abundance (P<0.05) of Megasphaera elsdenii in feces and about six times higher abundance (P<0.05) of Lactobacillus reuteri in ileal digesta than pigs fed the other diets.
25046106	4	39	theme	cannulated	743:752	arg1	pigs					762:765	seven post valve t-cecum cannulated growing pigs	718:765	seven post valve t-cecum cannulated growing pigs	718:765	The experiment comprised four diets and four periods in a change-over design with seven post valve t-cecum cannulated growing pigs.
25046106	8	40	theme	other	1651:1655	arg1	diets					1657:1661	the other diets	1647:1661	the other diets	1647:1661	In pigs fed diet CFO, three TRFs related to Prevotellaceae together consisted of more than 25% of the fecal microbiota, which is about 3 to 23 times higher (P<0.05) than in pigs fed the other diets.
25046106	10	41	dep	xylose	1943:1948	arg1	r=0.53					1951:1956	r=0.53	1951:1956	r=0.53; P=0.004	1951:1965	The total amount of digested NSP (r=0.57; P=0.002), xylose (r=0.53; P=0.004) and dietary fiber (r=0.60; P=0.001) in ileal digesta were positively correlated with an increased abundance of Bacteroides-Prevotella-Porphyromonas.
25046106	11	42	theme	fecal	2268:2272	arg1	acid					2282:2285	the fecal butyric acid	2264:2285	the fecal butyric acid proportion	2264:2296	The effect on SCFA was correlated to specific neutral sugars where xylose increased the ileal butyric acid proportion, whereas arabinose increased the fecal butyric acid proportion.
25046106	0	43	theme	pigs	143:146	arg1	community					122:130	ileal microbial community	106:130	ileal microbial community of growing pigs	106:146	Fermentable non-starch polysaccharides increases the abundance of Bacteroides-Prevotella-Porphyromonas in ileal microbial community of growing pigs.
25046106	5	44	theme	wheat	1017:1021	arg1	WB					1029:1030	WB	1029:1030	WB	1029:1030	The four diets were balanced to be similar in NSP content and included one of four fiber sources, two diets were rich in pectins, through inclusion of chicory forage (CFO) and sugar beet pulp, and two were rich in arabinoxylan, through inclusion of wheat bran (WB) and grass meal.
25046106	5	44	theme	wheat	1017:1021	arg1	bran					1023:1026	wheat bran	1017:1026	wheat bran (WB)	1017:1031	The four diets were balanced to be similar in NSP content and included one of four fiber sources, two diets were rich in pectins, through inclusion of chicory forage (CFO) and sugar beet pulp, and two were rich in arabinoxylan, through inclusion of wheat bran (WB) and grass meal.
25046106	10	45	dep	r=0.53	1951:1956	arg1	P=0.004					1959:1965	P=0.004	1959:1965	r=0.53; P=0.004	1951:1965	The total amount of digested NSP (r=0.57; P=0.002), xylose (r=0.53; P=0.004) and dietary fiber (r=0.60; P=0.001) in ileal digesta were positively correlated with an increased abundance of Bacteroides-Prevotella-Porphyromonas.
25046106	11	46	theme	specific	2154:2161	arg1	sugars					2171:2176	specific neutral sugars	2154:2176	specific neutral sugars where xylose increased the ileal butyric acid proportion, whereas arabinose increased the fecal butyric acid proportion	2154:2296	The effect on SCFA was correlated to specific neutral sugars where xylose increased the ileal butyric acid proportion, whereas arabinose increased the fecal butyric acid proportion.
25046106	0	47	theme	Fermentable	0:10	arg1	polysaccharides					23:37	Fermentable non-starch polysaccharides	0:37	Fermentable non-starch polysaccharides	0:37	Fermentable non-starch polysaccharides increases the abundance of Bacteroides-Prevotella-Porphyromonas in ileal microbial community of growing pigs.
25046106	10	48	theme	dietary	1972:1978	arg1	fiber					1980:1984	dietary fiber	1972:1984	dietary fiber (r=0.60; P=0.001)	1972:2002	The total amount of digested NSP (r=0.57; P=0.002), xylose (r=0.53; P=0.004) and dietary fiber (r=0.60; P=0.001) in ileal digesta were positively correlated with an increased abundance of Bacteroides-Prevotella-Porphyromonas.
25046106	4	49	theme	valve	729:733	arg1	pigs					762:765	seven post valve t-cecum cannulated growing pigs	718:765	seven post valve t-cecum cannulated growing pigs	718:765	The experiment comprised four diets and four periods in a change-over design with seven post valve t-cecum cannulated growing pigs.
25046106	11	50	theme	butyric	2211:2217	arg1	acid					2219:2222	the ileal butyric acid	2201:2222	the ileal butyric acid proportion	2201:2233	The effect on SCFA was correlated to specific neutral sugars where xylose increased the ileal butyric acid proportion, whereas arabinose increased the fecal butyric acid proportion.
25046106	3	51	theme	study	419:423	arg1	aim					407:409	The aim	403:409	The aim of this study	403:423	The aim of this study was to investigate effects of feeding diets with native sources of NSP on the ileal and fecal microbial composition and the dietary impact on the concentration of short-chain fatty acids (SCFA) and lactic acid.
25046106	10	52	theme	total	1895:1899	arg1	fiber					1980:1984	dietary fiber	1972:1984	dietary fiber (r=0.60; P=0.001)	1972:2002	The total amount of digested NSP (r=0.57; P=0.002), xylose (r=0.53; P=0.004) and dietary fiber (r=0.60; P=0.001) in ileal digesta were positively correlated with an increased abundance of Bacteroides-Prevotella-Porphyromonas.
25046106	10	52	theme	total	1895:1899	arg1	amount					1901:1906	The total amount	1891:1906	The total amount of digested NSP (r=0.57; P=0.002), xylose (r=0.53; P=0.004) and dietary fiber (r=0.60; P=0.001) in ileal digesta	1891:2019	The total amount of digested NSP (r=0.57; P=0.002), xylose (r=0.53; P=0.004) and dietary fiber (r=0.60; P=0.001) in ileal digesta were positively correlated with an increased abundance of Bacteroides-Prevotella-Porphyromonas.
25046106	10	52	theme	total	1895:1899	arg1	NSP					1920:1922	digested NSP	1911:1922	digested NSP (r=0.57; P=0.002)	1911:1940	The total amount of digested NSP (r=0.57; P=0.002), xylose (r=0.53; P=0.004) and dietary fiber (r=0.60; P=0.001) in ileal digesta were positively correlated with an increased abundance of Bacteroides-Prevotella-Porphyromonas.
25046106	10	52	theme	total	1895:1899	arg1	xylose					1943:1948	xylose	1943:1948	xylose (r=0.53; P=0.004)	1943:1966	The total amount of digested NSP (r=0.57; P=0.002), xylose (r=0.53; P=0.004) and dietary fiber (r=0.60; P=0.001) in ileal digesta were positively correlated with an increased abundance of Bacteroides-Prevotella-Porphyromonas.
25046106	3	53	theme	feeding	455:461	arg1	diets					463:467	feeding diets	455:467	feeding diets with native sources of NSP	455:494	The aim of this study was to investigate effects of feeding diets with native sources of NSP on the ileal and fecal microbial composition and the dietary impact on the concentration of short-chain fatty acids (SCFA) and lactic acid.
25046106	5	54	theme	grass	1037:1041	arg1	meal					1043:1046	grass meal	1037:1046	grass meal	1037:1046	The four diets were balanced to be similar in NSP content and included one of four fiber sources, two diets were rich in pectins, through inclusion of chicory forage (CFO) and sugar beet pulp, and two were rich in arabinoxylan, through inclusion of wheat bran (WB) and grass meal.
25046106	5	55	theme	beet	950:953	arg1	inclusion					906:914	inclusion	906:914	inclusion of chicory forage (CFO) and sugar beet pulp	906:958	The four diets were balanced to be similar in NSP content and included one of four fiber sources, two diets were rich in pectins, through inclusion of chicory forage (CFO) and sugar beet pulp, and two were rich in arabinoxylan, through inclusion of wheat bran (WB) and grass meal.
25046106	1	56	contain	contains	204:211	arg1	sources					173:179	Most plant-origin fiber sources	149:179	Most plant-origin fiber sources used in pig production	149:202	Most plant-origin fiber sources used in pig production contains a mixture of soluble and insoluble non-starch polysaccharides (NSP).
25046106	1	56	contain	contains	204:211	arg2	mixture					215:221	a mixture	213:221	a mixture of soluble and insoluble non-starch polysaccharides (NSP)	213:279	Most plant-origin fiber sources used in pig production contains a mixture of soluble and insoluble non-starch polysaccharides (NSP).
25046106	10	57	from	amount	1901:1906	arg1	digesta					2013:2019	ileal digesta	2007:2019	ileal digesta	2007:2019	The total amount of digested NSP (r=0.57; P=0.002), xylose (r=0.53; P=0.004) and dietary fiber (r=0.60; P=0.001) in ileal digesta were positively correlated with an increased abundance of Bacteroides-Prevotella-Porphyromonas.
25046106	3	58	theme	NSP	492:494	arg1	sources					481:487	native sources	474:487	native sources of NSP	474:494	The aim of this study was to investigate effects of feeding diets with native sources of NSP on the ileal and fecal microbial composition and the dietary impact on the concentration of short-chain fatty acids (SCFA) and lactic acid.
25046106	7	59	dep	structure	1369:1377	arg1	pectin					1396:1401	pectin	1396:1401	pectin	1396:1401	The gut microbiota did not cluster based on NSP structure (arabinoxylan or pectin) rather, the effect was to a high degree ingredient specific.
25046106	7	59	dep	structure	1369:1377	arg1	arabinoxylan					1380:1391	arabinoxylan	1380:1391	arabinoxylan	1380:1391	The gut microbiota did not cluster based on NSP structure (arabinoxylan or pectin) rather, the effect was to a high degree ingredient specific.
25046106	1	60	theme	polysaccharides	259:273	arg1	mixture					215:221	a mixture	213:221	a mixture of soluble and insoluble non-starch polysaccharides (NSP)	213:279	Most plant-origin fiber sources used in pig production contains a mixture of soluble and insoluble non-starch polysaccharides (NSP).
25046106	3	61	theme	diets	463:467	arg1	effects					444:450	effects	444:450	effects of feeding diets with native sources of NSP on the ileal and fecal microbial composition	444:539	The aim of this study was to investigate effects of feeding diets with native sources of NSP on the ileal and fecal microbial composition and the dietary impact on the concentration of short-chain fatty acids (SCFA) and lactic acid.
25046106	3	61	theme	diets	463:467	arg1	impact					557:562	the dietary impact	545:562	the dietary impact on the concentration of short-chain fatty acids (SCFA) and lactic acid	545:633	The aim of this study was to investigate effects of feeding diets with native sources of NSP on the ileal and fecal microbial composition and the dietary impact on the concentration of short-chain fatty acids (SCFA) and lactic acid.
25046106	5	62	from	pectins	889:895	arg1	rich					881:884	rich	881:884	rich	881:884	The four diets were balanced to be similar in NSP content and included one of four fiber sources, two diets were rich in pectins, through inclusion of chicory forage (CFO) and sugar beet pulp, and two were rich in arabinoxylan, through inclusion of wheat bran (WB) and grass meal.
25046106	6	63	theme	spp.	1188:1191	arg1	xynB					1278:1281	xynB	1278:1281	xynB	1278:1281	The gut microbial composition was assessed with terminal restriction fragment (TRF) length polymorphism and the abundance of Lactobacillus spp., Enterobacteriaceae, Bacteroides-Prevotella-Porphyromonas and the β-xylosidase gene, xynB, were assessed with quantitative PCR.
25046106	6	63	theme	spp.	1188:1191	arg1	Bacteroides-Prevotella-Porphyromonas					1214:1249	Bacteroides-Prevotella-Porphyromonas	1214:1249	Bacteroides-Prevotella-Porphyromonas	1214:1249	The gut microbial composition was assessed with terminal restriction fragment (TRF) length polymorphism and the abundance of Lactobacillus spp., Enterobacteriaceae, Bacteroides-Prevotella-Porphyromonas and the β-xylosidase gene, xynB, were assessed with quantitative PCR.
25046106	6	63	theme	spp.	1188:1191	arg1	Enterobacteriaceae					1194:1211	Enterobacteriaceae	1194:1211	Enterobacteriaceae	1194:1211	The gut microbial composition was assessed with terminal restriction fragment (TRF) length polymorphism and the abundance of Lactobacillus spp., Enterobacteriaceae, Bacteroides-Prevotella-Porphyromonas and the β-xylosidase gene, xynB, were assessed with quantitative PCR.
25046106	6	63	theme	spp.	1188:1191	arg1	abundance					1161:1169	the abundance	1157:1169	the abundance of Lactobacillus spp.	1157:1191	The gut microbial composition was assessed with terminal restriction fragment (TRF) length polymorphism and the abundance of Lactobacillus spp., Enterobacteriaceae, Bacteroides-Prevotella-Porphyromonas and the β-xylosidase gene, xynB, were assessed with quantitative PCR.
25046106	6	63	theme	spp.	1188:1191	arg1	gene					1272:1275	the β-xylosidase gene	1255:1275	the β-xylosidase gene	1255:1275	The gut microbial composition was assessed with terminal restriction fragment (TRF) length polymorphism and the abundance of Lactobacillus spp., Enterobacteriaceae, Bacteroides-Prevotella-Porphyromonas and the β-xylosidase gene, xynB, were assessed with quantitative PCR.
25046106	3	64	theme	lactic	623:628	arg1	acid					630:633	lactic acid	623:633	lactic acid	623:633	The aim of this study was to investigate effects of feeding diets with native sources of NSP on the ileal and fecal microbial composition and the dietary impact on the concentration of short-chain fatty acids (SCFA) and lactic acid.
25046106	8	65	dep	times	1608:1612	arg1	higher					1614:1619	higher	1614:1619	higher	1614:1619	In pigs fed diet CFO, three TRFs related to Prevotellaceae together consisted of more than 25% of the fecal microbiota, which is about 3 to 23 times higher (P<0.05) than in pigs fed the other diets.
25046106	8	65	dep	times	1608:1612	arg1	P<0.05					1622:1627	P<0.05	1622:1627	P<0.05	1622:1627	In pigs fed diet CFO, three TRFs related to Prevotellaceae together consisted of more than 25% of the fecal microbiota, which is about 3 to 23 times higher (P<0.05) than in pigs fed the other diets.
25046106	8	65	dep	times	1608:1612	arg1	to					1602:1603	to	1602:1603	to	1602:1603	In pigs fed diet CFO, three TRFs related to Prevotellaceae together consisted of more than 25% of the fecal microbiota, which is about 3 to 23 times higher (P<0.05) than in pigs fed the other diets.
25046106	8	66	theme	related	1498:1504	arg1	TRFs					1493:1496	three TRFs	1487:1496	three TRFs related to Prevotellaceae	1487:1522	In pigs fed diet CFO, three TRFs related to Prevotellaceae together consisted of more than 25% of the fecal microbiota, which is about 3 to 23 times higher (P<0.05) than in pigs fed the other diets.
25046106	2	67	theme	sources	319:325	arg1	effects					302:308	effects	302:308	effects of these sources of NSP on the gut microbiota and its fermentation products	302:384	The knowledge about effects of these sources of NSP on the gut microbiota and its fermentation products is still scarce.
25046106	5	68	theme	sources	857:863	arg1	sources					857:863	four fiber sources	846:863	four fiber sources	846:863	The four diets were balanced to be similar in NSP content and included one of four fiber sources, two diets were rich in pectins, through inclusion of chicory forage (CFO) and sugar beet pulp, and two were rich in arabinoxylan, through inclusion of wheat bran (WB) and grass meal.
25046106	5	68	theme	sources	857:863	arg1	one					839:841	one	839:841	one	839:841	The four diets were balanced to be similar in NSP content and included one of four fiber sources, two diets were rich in pectins, through inclusion of chicory forage (CFO) and sugar beet pulp, and two were rich in arabinoxylan, through inclusion of wheat bran (WB) and grass meal.
25046106	3	69	theme	microbial	519:527	arg1	composition					529:539	the ileal and fecal microbial composition	499:539	the ileal and fecal microbial composition	499:539	The aim of this study was to investigate effects of feeding diets with native sources of NSP on the ileal and fecal microbial composition and the dietary impact on the concentration of short-chain fatty acids (SCFA) and lactic acid.
25046106	8	70	theme	fecal	1567:1571	arg1	microbiota					1573:1582	the fecal microbiota	1563:1582	the fecal microbiota	1563:1582	In pigs fed diet CFO, three TRFs related to Prevotellaceae together consisted of more than 25% of the fecal microbiota, which is about 3 to 23 times higher (P<0.05) than in pigs fed the other diets.
25046106	8	70	theme	fecal	1567:1571	arg1	times					1608:1612	times	1608:1612	times	1608:1612	In pigs fed diet CFO, three TRFs related to Prevotellaceae together consisted of more than 25% of the fecal microbiota, which is about 3 to 23 times higher (P<0.05) than in pigs fed the other diets.
25046106	6	71	theme	gut	1053:1055	arg1	composition					1067:1077	The gut microbial composition	1049:1077	The gut microbial composition	1049:1077	The gut microbial composition was assessed with terminal restriction fragment (TRF) length polymorphism and the abundance of Lactobacillus spp., Enterobacteriaceae, Bacteroides-Prevotella-Porphyromonas and the β-xylosidase gene, xynB, were assessed with quantitative PCR.
25046106	10	72	theme	ileal	2007:2011	arg1	digesta					2013:2019	ileal digesta	2007:2019	ileal digesta	2007:2019	The total amount of digested NSP (r=0.57; P=0.002), xylose (r=0.53; P=0.004) and dietary fiber (r=0.60; P=0.001) in ileal digesta were positively correlated with an increased abundance of Bacteroides-Prevotella-Porphyromonas.
25046106	3	73	theme	dietary	549:555	arg1	impact					557:562	the dietary impact	545:562	the dietary impact on the concentration of short-chain fatty acids (SCFA) and lactic acid	545:633	The aim of this study was to investigate effects of feeding diets with native sources of NSP on the ileal and fecal microbial composition and the dietary impact on the concentration of short-chain fatty acids (SCFA) and lactic acid.
25046106	1	74	theme	soluble	226:232	arg1	NSP					276:278	NSP	276:278	NSP	276:278	Most plant-origin fiber sources used in pig production contains a mixture of soluble and insoluble non-starch polysaccharides (NSP).
25046106	1	74	theme	soluble	226:232	arg1	polysaccharides					259:273	soluble and insoluble non-starch polysaccharides	226:273	soluble and insoluble non-starch polysaccharides (NSP)	226:279	Most plant-origin fiber sources used in pig production contains a mixture of soluble and insoluble non-starch polysaccharides (NSP).
25046106	10	75	theme	digested	1911:1918	arg1	NSP					1920:1922	digested NSP	1911:1922	digested NSP (r=0.57; P=0.002)	1911:1940	The total amount of digested NSP (r=0.57; P=0.002), xylose (r=0.53; P=0.004) and dietary fiber (r=0.60; P=0.001) in ileal digesta were positively correlated with an increased abundance of Bacteroides-Prevotella-Porphyromonas.
25046106	3	76	theme	short-chain	588:598	arg1	SCFA					613:616	SCFA	613:616	SCFA	613:616	The aim of this study was to investigate effects of feeding diets with native sources of NSP on the ileal and fecal microbial composition and the dietary impact on the concentration of short-chain fatty acids (SCFA) and lactic acid.
25046106	3	76	theme	short-chain	588:598	arg1	acids					606:610	short-chain fatty acids	588:610	short-chain fatty acids (SCFA)	588:617	The aim of this study was to investigate effects of feeding diets with native sources of NSP on the ileal and fecal microbial composition and the dietary impact on the concentration of short-chain fatty acids (SCFA) and lactic acid.
25046106	8	77	theme	3	1600:1600	arg1	times					1608:1612	times	1608:1612	times	1608:1612	In pigs fed diet CFO, three TRFs related to Prevotellaceae together consisted of more than 25% of the fecal microbiota, which is about 3 to 23 times higher (P<0.05) than in pigs fed the other diets.
25046106	8	77	theme	3	1600:1600	arg1	microbiota					1573:1582	the fecal microbiota	1563:1582	the fecal microbiota	1563:1582	In pigs fed diet CFO, three TRFs related to Prevotellaceae together consisted of more than 25% of the fecal microbiota, which is about 3 to 23 times higher (P<0.05) than in pigs fed the other diets.
25046106	5	78	theme	NSP	814:816	arg1	content					818:824	NSP content	814:824	NSP content	814:824	The four diets were balanced to be similar in NSP content and included one of four fiber sources, two diets were rich in pectins, through inclusion of chicory forage (CFO) and sugar beet pulp, and two were rich in arabinoxylan, through inclusion of wheat bran (WB) and grass meal.
25046106	11	79	theme	butyric	2274:2280	arg1	acid					2282:2285	the fecal butyric acid	2264:2285	the fecal butyric acid proportion	2264:2296	The effect on SCFA was correlated to specific neutral sugars where xylose increased the ileal butyric acid proportion, whereas arabinose increased the fecal butyric acid proportion.
25046106	6	80	theme	TRF	1128:1130	arg1	polymorphism					1140:1151	terminal restriction fragment (TRF) length polymorphism	1097:1151	terminal restriction fragment (TRF) length polymorphism	1097:1151	The gut microbial composition was assessed with terminal restriction fragment (TRF) length polymorphism and the abundance of Lactobacillus spp., Enterobacteriaceae, Bacteroides-Prevotella-Porphyromonas and the β-xylosidase gene, xynB, were assessed with quantitative PCR.
25046106	5	81	from	content	818:824	arg1	similar					803:809	similar	803:809	similar	803:809	The four diets were balanced to be similar in NSP content and included one of four fiber sources, two diets were rich in pectins, through inclusion of chicory forage (CFO) and sugar beet pulp, and two were rich in arabinoxylan, through inclusion of wheat bran (WB) and grass meal.
25046106	1	82	theme	Most	149:152	arg1	sources					173:179	Most plant-origin fiber sources	149:179	Most plant-origin fiber sources used in pig production	149:202	Most plant-origin fiber sources used in pig production contains a mixture of soluble and insoluble non-starch polysaccharides (NSP).
25046106	10	83	theme	Bacteroides-Prevotella-Porphyromonas	2079:2114	arg1	abundance					2066:2074	an increased abundance	2053:2074	an increased abundance of Bacteroides-Prevotella-Porphyromonas	2053:2114	The total amount of digested NSP (r=0.57; P=0.002), xylose (r=0.53; P=0.004) and dietary fiber (r=0.60; P=0.001) in ileal digesta were positively correlated with an increased abundance of Bacteroides-Prevotella-Porphyromonas.
25046106	6	84	theme	fragment	1118:1125	arg1	polymorphism					1140:1151	terminal restriction fragment (TRF) length polymorphism	1097:1151	terminal restriction fragment (TRF) length polymorphism	1097:1151	The gut microbial composition was assessed with terminal restriction fragment (TRF) length polymorphism and the abundance of Lactobacillus spp., Enterobacteriaceae, Bacteroides-Prevotella-Porphyromonas and the β-xylosidase gene, xynB, were assessed with quantitative PCR.
25046106	11	85	theme	neutral	2163:2169	arg1	sugars					2171:2176	specific neutral sugars	2154:2176	specific neutral sugars where xylose increased the ileal butyric acid proportion, whereas arabinose increased the fecal butyric acid proportion	2154:2296	The effect on SCFA was correlated to specific neutral sugars where xylose increased the ileal butyric acid proportion, whereas arabinose increased the fecal butyric acid proportion.
25046106	5	86	from	rich	974:977	arg1	arabinoxylan					982:993	arabinoxylan	982:993	arabinoxylan	982:993	The four diets were balanced to be similar in NSP content and included one of four fiber sources, two diets were rich in pectins, through inclusion of chicory forage (CFO) and sugar beet pulp, and two were rich in arabinoxylan, through inclusion of wheat bran (WB) and grass meal.
25046106	1	87	theme	fiber	167:171	arg1	sources					173:179	Most plant-origin fiber sources	149:179	Most plant-origin fiber sources used in pig production	149:202	Most plant-origin fiber sources used in pig production contains a mixture of soluble and insoluble non-starch polysaccharides (NSP).
25046106	2	88	theme	NSP	330:332	arg1	sources					319:325	these sources	313:325	these sources of NSP	313:332	The knowledge about effects of these sources of NSP on the gut microbiota and its fermentation products is still scarce.
25046106	6	89	theme	terminal	1097:1104	arg1	polymorphism					1140:1151	terminal restriction fragment (TRF) length polymorphism	1097:1151	terminal restriction fragment (TRF) length polymorphism	1097:1151	The gut microbial composition was assessed with terminal restriction fragment (TRF) length polymorphism and the abundance of Lactobacillus spp., Enterobacteriaceae, Bacteroides-Prevotella-Porphyromonas and the β-xylosidase gene, xynB, were assessed with quantitative PCR.
25046106	11	90	theme	ileal	2205:2209	arg1	acid					2219:2222	the ileal butyric acid	2201:2222	the ileal butyric acid proportion	2201:2233	The effect on SCFA was correlated to specific neutral sugars where xylose increased the ileal butyric acid proportion, whereas arabinose increased the fecal butyric acid proportion.
25046106	4	91	theme	change-over	694:704	arg1	design					706:711	a change-over design	692:711	a change-over design with seven post valve t-cecum cannulated growing pigs	692:765	The experiment comprised four diets and four periods in a change-over design with seven post valve t-cecum cannulated growing pigs.
25046106	0	92	theme	ileal	106:110	arg1	community					122:130	ileal microbial community	106:130	ileal microbial community of growing pigs	106:146	Fermentable non-starch polysaccharides increases the abundance of Bacteroides-Prevotella-Porphyromonas in ileal microbial community of growing pigs.
25046106	3	93	theme	acids	606:610	arg1	concentration					571:583	the concentration	567:583	the concentration of short-chain fatty acids (SCFA) and lactic acid	567:633	The aim of this study was to investigate effects of feeding diets with native sources of NSP on the ileal and fecal microbial composition and the dietary impact on the concentration of short-chain fatty acids (SCFA) and lactic acid.
25046106	9	94	dep	Megasphaera	1742:1752	arg1	elsdenii					1754:1761	Megasphaera elsdenii	1742:1761	Megasphaera elsdenii	1742:1761	Whereas pigs fed diet WB had about 2 to 22 times higher abundance (P<0.05) of Megasphaera elsdenii in feces and about six times higher abundance (P<0.05) of Lactobacillus reuteri in ileal digesta than pigs fed the other diets.
25046106	10	95	theme	xylose	1943:1948	arg1	fiber					1980:1984	dietary fiber	1972:1984	dietary fiber (r=0.60; P=0.001)	1972:2002	The total amount of digested NSP (r=0.57; P=0.002), xylose (r=0.53; P=0.004) and dietary fiber (r=0.60; P=0.001) in ileal digesta were positively correlated with an increased abundance of Bacteroides-Prevotella-Porphyromonas.
25046106	10	95	theme	xylose	1943:1948	arg1	amount					1901:1906	The total amount	1891:1906	The total amount of digested NSP (r=0.57; P=0.002), xylose (r=0.53; P=0.004) and dietary fiber (r=0.60; P=0.001) in ileal digesta	1891:2019	The total amount of digested NSP (r=0.57; P=0.002), xylose (r=0.53; P=0.004) and dietary fiber (r=0.60; P=0.001) in ileal digesta were positively correlated with an increased abundance of Bacteroides-Prevotella-Porphyromonas.
25046106	10	95	theme	xylose	1943:1948	arg1	NSP					1920:1922	digested NSP	1911:1922	digested NSP (r=0.57; P=0.002)	1911:1940	The total amount of digested NSP (r=0.57; P=0.002), xylose (r=0.53; P=0.004) and dietary fiber (r=0.60; P=0.001) in ileal digesta were positively correlated with an increased abundance of Bacteroides-Prevotella-Porphyromonas.
25046106	10	95	theme	xylose	1943:1948	arg1	xylose					1943:1948	xylose	1943:1948	xylose (r=0.53; P=0.004)	1943:1966	The total amount of digested NSP (r=0.57; P=0.002), xylose (r=0.53; P=0.004) and dietary fiber (r=0.60; P=0.001) in ileal digesta were positively correlated with an increased abundance of Bacteroides-Prevotella-Porphyromonas.
25046106	1	96	theme	pig	189:191	arg1	production					193:202	pig production	189:202	pig production	189:202	Most plant-origin fiber sources used in pig production contains a mixture of soluble and insoluble non-starch polysaccharides (NSP).
25046106	7	97	theme	specific	1455:1462	arg1	degree					1437:1442	a high degree	1430:1442	a high degree ingredient specific	1430:1462	The gut microbiota did not cluster based on NSP structure (arabinoxylan or pectin) rather, the effect was to a high degree ingredient specific.
25046106	7	97	theme	specific	1455:1462	arg1	effect					1416:1421	the effect	1412:1421	the effect	1412:1421	The gut microbiota did not cluster based on NSP structure (arabinoxylan or pectin) rather, the effect was to a high degree ingredient specific.
25046106	3	98	with	diets	463:467	arg1	sources					481:487	native sources	474:487	native sources of NSP	474:494	The aim of this study was to investigate effects of feeding diets with native sources of NSP on the ileal and fecal microbial composition and the dietary impact on the concentration of short-chain fatty acids (SCFA) and lactic acid.
25046106	11	99	theme	acid	2219:2222	arg1	proportion					2224:2233	the ileal butyric acid proportion	2201:2233	the ileal butyric acid proportion	2201:2233	The effect on SCFA was correlated to specific neutral sugars where xylose increased the ileal butyric acid proportion, whereas arabinose increased the fecal butyric acid proportion.
25046106	0	100	theme	growing	135:141	arg1	pigs					143:146	growing pigs	135:146	growing pigs	135:146	Fermentable non-starch polysaccharides increases the abundance of Bacteroides-Prevotella-Porphyromonas in ileal microbial community of growing pigs.
25046106	9	101	dep	had	1689:1691	arg1	Whereas					1664:1670	Whereas	1664:1670	Whereas	1664:1670	Whereas pigs fed diet WB had about 2 to 22 times higher abundance (P<0.05) of Megasphaera elsdenii in feces and about six times higher abundance (P<0.05) of Lactobacillus reuteri in ileal digesta than pigs fed the other diets.
25046106	12	102	theme	ileal	2369:2373	arg1	digesta					2375:2381	ileal digesta	2369:2381	ileal digesta	2369:2381	Moreover, chicory pectin increased the acetic acid proportion in both ileal digesta and feces.
25046106	4	103	theme	t-cecum	735:741	arg1	pigs					762:765	seven post valve t-cecum cannulated growing pigs	718:765	seven post valve t-cecum cannulated growing pigs	718:765	The experiment comprised four diets and four periods in a change-over design with seven post valve t-cecum cannulated growing pigs.
25046106	5	104	theme	chicory	919:925	arg1	inclusion					906:914	inclusion	906:914	inclusion of chicory forage (CFO) and sugar beet pulp	906:958	The four diets were balanced to be similar in NSP content and included one of four fiber sources, two diets were rich in pectins, through inclusion of chicory forage (CFO) and sugar beet pulp, and two were rich in arabinoxylan, through inclusion of wheat bran (WB) and grass meal.
25046106	0	105	from	abundance	53:61	arg1	community					122:130	ileal microbial community	106:130	ileal microbial community of growing pigs	106:146	Fermentable non-starch polysaccharides increases the abundance of Bacteroides-Prevotella-Porphyromonas in ileal microbial community of growing pigs.
25046106	4	106	theme	growing	754:760	arg1	pigs					762:765	seven post valve t-cecum cannulated growing pigs	718:765	seven post valve t-cecum cannulated growing pigs	718:765	The experiment comprised four diets and four periods in a change-over design with seven post valve t-cecum cannulated growing pigs.
25046106	1	107	theme	insoluble	238:246	arg1	NSP					276:278	NSP	276:278	NSP	276:278	Most plant-origin fiber sources used in pig production contains a mixture of soluble and insoluble non-starch polysaccharides (NSP).
25046106	1	107	theme	insoluble	238:246	arg1	polysaccharides					259:273	soluble and insoluble non-starch polysaccharides	226:273	soluble and insoluble non-starch polysaccharides (NSP)	226:279	Most plant-origin fiber sources used in pig production contains a mixture of soluble and insoluble non-starch polysaccharides (NSP).
25046106	0	108	theme	non-starch	12:21	arg1	polysaccharides					23:37	Fermentable non-starch polysaccharides	0:37	Fermentable non-starch polysaccharides	0:37	Fermentable non-starch polysaccharides increases the abundance of Bacteroides-Prevotella-Porphyromonas in ileal microbial community of growing pigs.
25046106	5	109	theme	bran	1023:1026	arg1	inclusion					1004:1012	inclusion	1004:1012	inclusion of wheat bran (WB) and grass meal	1004:1046	The four diets were balanced to be similar in NSP content and included one of four fiber sources, two diets were rich in pectins, through inclusion of chicory forage (CFO) and sugar beet pulp, and two were rich in arabinoxylan, through inclusion of wheat bran (WB) and grass meal.
25046106	9	110	theme	ileal	1846:1850	arg1	digesta					1852:1858	ileal digesta	1846:1858	ileal digesta	1846:1858	Whereas pigs fed diet WB had about 2 to 22 times higher abundance (P<0.05) of Megasphaera elsdenii in feces and about six times higher abundance (P<0.05) of Lactobacillus reuteri in ileal digesta than pigs fed the other diets.
25046106	5	111	from	rich	881:884	arg1	pectins					889:895	pectins	889:895	pectins	889:895	The four diets were balanced to be similar in NSP content and included one of four fiber sources, two diets were rich in pectins, through inclusion of chicory forage (CFO) and sugar beet pulp, and two were rich in arabinoxylan, through inclusion of wheat bran (WB) and grass meal.
25046106	9	112	theme	reuteri	1835:1841	arg1	abundance					1720:1728	about 2 to 22 times higher abundance	1693:1728	about 2 to 22 times higher abundance (P<0.05) of Megasphaera elsdenii in feces	1693:1770	Whereas pigs fed diet WB had about 2 to 22 times higher abundance (P<0.05) of Megasphaera elsdenii in feces and about six times higher abundance (P<0.05) of Lactobacillus reuteri in ileal digesta than pigs fed the other diets.
25046106	9	112	theme	reuteri	1835:1841	arg1	P<0.05					1810:1815	P<0.05	1810:1815	P<0.05	1810:1815	Whereas pigs fed diet WB had about 2 to 22 times higher abundance (P<0.05) of Megasphaera elsdenii in feces and about six times higher abundance (P<0.05) of Lactobacillus reuteri in ileal digesta than pigs fed the other diets.
25046106	9	112	theme	reuteri	1835:1841	arg1	abundance					1799:1807	about six times higher abundance	1776:1807	about six times higher abundance (P<0.05)	1776:1816	Whereas pigs fed diet WB had about 2 to 22 times higher abundance (P<0.05) of Megasphaera elsdenii in feces and about six times higher abundance (P<0.05) of Lactobacillus reuteri in ileal digesta than pigs fed the other diets.
25046106	9	112	theme	reuteri	1835:1841	arg1	P<0.05					1731:1736	P<0.05	1731:1736	P<0.05	1731:1736	Whereas pigs fed diet WB had about 2 to 22 times higher abundance (P<0.05) of Megasphaera elsdenii in feces and about six times higher abundance (P<0.05) of Lactobacillus reuteri in ileal digesta than pigs fed the other diets.
25046106	10	113	dep	r=0.57	1925:1930	arg1	P=0.002					1933:1939	P=0.002	1933:1939	r=0.57; P=0.002	1925:1939	The total amount of digested NSP (r=0.57; P=0.002), xylose (r=0.53; P=0.004) and dietary fiber (r=0.60; P=0.001) in ileal digesta were positively correlated with an increased abundance of Bacteroides-Prevotella-Porphyromonas.
25046106	3	114	theme	acid	630:633	arg1	concentration					571:583	the concentration	567:583	the concentration of short-chain fatty acids (SCFA) and lactic acid	567:633	The aim of this study was to investigate effects of feeding diets with native sources of NSP on the ileal and fecal microbial composition and the dietary impact on the concentration of short-chain fatty acids (SCFA) and lactic acid.
25046106	4	115	theme	post	724:727	arg1	pigs					762:765	seven post valve t-cecum cannulated growing pigs	718:765	seven post valve t-cecum cannulated growing pigs	718:765	The experiment comprised four diets and four periods in a change-over design with seven post valve t-cecum cannulated growing pigs.
25046106	3	116	theme	native	474:479	arg1	sources					481:487	native sources	474:487	native sources of NSP	474:494	The aim of this study was to investigate effects of feeding diets with native sources of NSP on the ileal and fecal microbial composition and the dietary impact on the concentration of short-chain fatty acids (SCFA) and lactic acid.
25046106	7	117	theme	high	1432:1435	arg1	degree					1437:1442	a high degree	1430:1442	a high degree ingredient specific	1430:1462	The gut microbiota did not cluster based on NSP structure (arabinoxylan or pectin) rather, the effect was to a high degree ingredient specific.
25046106	7	117	theme	high	1432:1435	arg1	effect					1416:1421	the effect	1412:1421	the effect	1412:1421	The gut microbiota did not cluster based on NSP structure (arabinoxylan or pectin) rather, the effect was to a high degree ingredient specific.
25046106	5	118	theme	sugar	944:948	arg1	beet					950:953	sugar beet	944:953	sugar beet	944:953	The four diets were balanced to be similar in NSP content and included one of four fiber sources, two diets were rich in pectins, through inclusion of chicory forage (CFO) and sugar beet pulp, and two were rich in arabinoxylan, through inclusion of wheat bran (WB) and grass meal.
25046106	5	119	from	arabinoxylan	982:993	arg1	rich					974:977	rich	974:977	rich	974:977	The four diets were balanced to be similar in NSP content and included one of four fiber sources, two diets were rich in pectins, through inclusion of chicory forage (CFO) and sugar beet pulp, and two were rich in arabinoxylan, through inclusion of wheat bran (WB) and grass meal.
25046106	0	120	theme	Bacteroides-Prevotella-Porphyromonas	66:101	arg1	abundance					53:61	the abundance	49:61	the abundance of Bacteroides-Prevotella-Porphyromonas in ileal microbial community of growing pigs	49:146	Fermentable non-starch polysaccharides increases the abundance of Bacteroides-Prevotella-Porphyromonas in ileal microbial community of growing pigs.
25046106	5	121	theme	meal	1043:1046	arg1	inclusion					1004:1012	inclusion	1004:1012	inclusion of wheat bran (WB) and grass meal	1004:1046	The four diets were balanced to be similar in NSP content and included one of four fiber sources, two diets were rich in pectins, through inclusion of chicory forage (CFO) and sugar beet pulp, and two were rich in arabinoxylan, through inclusion of wheat bran (WB) and grass meal.
25046106	8	122	theme	diet	1477:1480	arg1	CFO					1482:1484	diet CFO	1477:1484	diet CFO	1477:1484	In pigs fed diet CFO, three TRFs related to Prevotellaceae together consisted of more than 25% of the fecal microbiota, which is about 3 to 23 times higher (P<0.05) than in pigs fed the other diets.
25046106	10	123	dep	NSP	1920:1922	arg1	r=0.57					1925:1930	r=0.57	1925:1930	r=0.57	1925:1930	The total amount of digested NSP (r=0.57; P=0.002), xylose (r=0.53; P=0.004) and dietary fiber (r=0.60; P=0.001) in ileal digesta were positively correlated with an increased abundance of Bacteroides-Prevotella-Porphyromonas.
25046106	9	124	theme	diet	1681:1684	arg1	WB					1686:1687	diet WB	1681:1687	diet WB	1681:1687	Whereas pigs fed diet WB had about 2 to 22 times higher abundance (P<0.05) of Megasphaera elsdenii in feces and about six times higher abundance (P<0.05) of Lactobacillus reuteri in ileal digesta than pigs fed the other diets.
25046106	9	125	theme	other	1878:1882	arg1	diets					1884:1888	the other diets	1874:1888	the other diets	1874:1888	Whereas pigs fed diet WB had about 2 to 22 times higher abundance (P<0.05) of Megasphaera elsdenii in feces and about six times higher abundance (P<0.05) of Lactobacillus reuteri in ileal digesta than pigs fed the other diets.
25046106	2	126	theme	gut	341:343	arg1	microbiota					345:354	the gut microbiota	337:354	the gut microbiota	337:354	The knowledge about effects of these sources of NSP on the gut microbiota and its fermentation products is still scarce.
25046106	9	127	dep	abundance	1799:1807	arg1	times					1786:1790	times	1786:1790	times	1786:1790	Whereas pigs fed diet WB had about 2 to 22 times higher abundance (P<0.05) of Megasphaera elsdenii in feces and about six times higher abundance (P<0.05) of Lactobacillus reuteri in ileal digesta than pigs fed the other diets.
25046106	12	128	theme	chicory	2309:2315	arg1	pectin					2317:2322	chicory pectin	2309:2322	chicory pectin	2309:2322	Moreover, chicory pectin increased the acetic acid proportion in both ileal digesta and feces.
25046106	4	129	with	design	706:711	arg1	pigs					762:765	seven post valve t-cecum cannulated growing pigs	718:765	seven post valve t-cecum cannulated growing pigs	718:765	The experiment comprised four diets and four periods in a change-over design with seven post valve t-cecum cannulated growing pigs.
25046106	3	130	from	effects	444:450	arg1	concentration					571:583	the concentration	567:583	the concentration of short-chain fatty acids (SCFA) and lactic acid	567:633	The aim of this study was to investigate effects of feeding diets with native sources of NSP on the ileal and fecal microbial composition and the dietary impact on the concentration of short-chain fatty acids (SCFA) and lactic acid.
25046106	3	130	from	effects	444:450	arg1	composition					529:539	the ileal and fecal microbial composition	499:539	the ileal and fecal microbial composition	499:539	The aim of this study was to investigate effects of feeding diets with native sources of NSP on the ileal and fecal microbial composition and the dietary impact on the concentration of short-chain fatty acids (SCFA) and lactic acid.
25046106	3	131	theme	fecal	513:517	arg1	composition					529:539	the ileal and fecal microbial composition	499:539	the ileal and fecal microbial composition	499:539	The aim of this study was to investigate effects of feeding diets with native sources of NSP on the ileal and fecal microbial composition and the dietary impact on the concentration of short-chain fatty acids (SCFA) and lactic acid.
26468288	0	0	theme	Liquid	94:99	arg1	Chromatography					101:114	Ultra Performance Liquid Chromatography	76:114	Ultra Performance Liquid Chromatography	76:114	High-throughput, Highly Sensitive Analyses of Bacterial Morphogenesis Using Ultra Performance Liquid Chromatography.
26468288	5	1	theme	muropeptide	740:750	arg1	analysis					752:759	muropeptide analysis	740:759	muropeptide analysis using ultra performance liquid chromatography (UPLC)	740:812	We present a protocol for muropeptide analysis using ultra performance liquid chromatography (UPLC) and demonstrate that UPLC achieves resolution comparable with that of HPLC while requiring orders of magnitude less injection volume and a fraction of the elution time.
26468288	8	2	theme	peptidoglycan	1608:1620	arg1	synthesis					1622:1630	peptidoglycan synthesis	1608:1630	peptidoglycan synthesis	1608:1630	Peptidoglycan composition and density were maintained after we systematically altered cell size in Escherichia coli using the antibiotic A22, indicating that cell shape is largely decoupled from the biochemistry of peptidoglycan synthesis.
26468288	1	3	theme	glycan	157:162	arg1	strands					164:170	glycan strands	157:170	glycan strands cross-linked by short peptides (peptidoglycan)	157:217	The bacterial cell wall is a network of glycan strands cross-linked by short peptides (peptidoglycan); it is responsible for the mechanical integrity of the cell and shape determination.
26468288	9	4	theme	determination	1842:1854	arg1	discovery					1751:1759	the discovery	1747:1759	the discovery of peptidoglycan composition	1747:1788	High-throughput, sensitive UPLC combined with our automated software for chromatographic analysis will accelerate the discovery of peptidoglycan composition and the molecular mechanisms of cell wall structure determination.
26468288	9	4	theme	determination	1842:1854	arg1	mechanisms					1808:1817	the molecular mechanisms	1794:1817	the molecular mechanisms of cell wall structure determination	1794:1854	High-throughput, sensitive UPLC combined with our automated software for chromatographic analysis will accelerate the discovery of peptidoglycan composition and the molecular mechanisms of cell wall structure determination.
26468288	0	5	theme	Performance	82:92	arg1	Chromatography					101:114	Ultra Performance Liquid Chromatography	76:114	Ultra Performance Liquid Chromatography	76:114	High-throughput, Highly Sensitive Analyses of Bacterial Morphogenesis Using Ultra Performance Liquid Chromatography.
26468288	1	6	theme	strands	164:170	arg1	wall					136:139	The bacterial cell wall	117:139	The bacterial cell wall	117:139	The bacterial cell wall is a network of glycan strands cross-linked by short peptides (peptidoglycan); it is responsible for the mechanical integrity of the cell and shape determination.
26468288	1	6	theme	strands	164:170	arg1	network					146:152	a network	144:152	a network of glycan strands cross-linked by short peptides (peptidoglycan)	144:217	The bacterial cell wall is a network of glycan strands cross-linked by short peptides (peptidoglycan); it is responsible for the mechanical integrity of the cell and shape determination.
26468288	1	7	theme	mechanical	246:255	arg1	integrity					257:265	the mechanical integrity	242:265	the mechanical integrity of the cell and shape determination	242:301	The bacterial cell wall is a network of glycan strands cross-linked by short peptides (peptidoglycan); it is responsible for the mechanical integrity of the cell and shape determination.
26468288	6	8	theme	peaks	1090:1094	arg1	quantification					1056:1069	quantification	1056:1069	quantification	1056:1069	We also developed a software platform to automate the identification and quantification of chromatographic peaks, which we demonstrate has improved accuracy relative to other software.
26468288	6	8	theme	peaks	1090:1094	arg1	identification					1037:1050	identification	1037:1050	identification	1037:1050	We also developed a software platform to automate the identification and quantification of chromatographic peaks, which we demonstrate has improved accuracy relative to other software.
26468288	1	9	dep	cell	274:277	arg1	the					270:272	the	270:272	the	270:272	The bacterial cell wall is a network of glycan strands cross-linked by short peptides (peptidoglycan); it is responsible for the mechanical integrity of the cell and shape determination.
26468288	1	9	dep	cell	274:277	arg1	determination					289:301	determination	289:301	determination	289:301	The bacterial cell wall is a network of glycan strands cross-linked by short peptides (peptidoglycan); it is responsible for the mechanical integrity of the cell and shape determination.
26468288	9	10	theme	peptidoglycan	1764:1776	arg1	composition					1778:1788	peptidoglycan composition	1764:1788	peptidoglycan composition	1764:1788	High-throughput, sensitive UPLC combined with our automated software for chromatographic analysis will accelerate the discovery of peptidoglycan composition and the molecular mechanisms of cell wall structure determination.
26468288	6	11	theme	other	1152:1156	arg1	software					1158:1165	other software	1152:1165	other software	1152:1165	We also developed a software platform to automate the identification and quantification of chromatographic peaks, which we demonstrate has improved accuracy relative to other software.
26468288	9	12	theme	composition	1778:1788	arg1	discovery					1751:1759	the discovery	1747:1759	the discovery of peptidoglycan composition	1747:1788	High-throughput, sensitive UPLC combined with our automated software for chromatographic analysis will accelerate the discovery of peptidoglycan composition and the molecular mechanisms of cell wall structure determination.
26468288	9	12	theme	composition	1778:1788	arg1	mechanisms					1808:1817	the molecular mechanisms	1794:1817	the molecular mechanisms of cell wall structure determination	1794:1854	High-throughput, sensitive UPLC combined with our automated software for chromatographic analysis will accelerate the discovery of peptidoglycan composition and the molecular mechanisms of cell wall structure determination.
26468288	5	13	dep	less	925:928	arg1	volume					940:945	injection volume	930:945	injection volume	930:945	We present a protocol for muropeptide analysis using ultra performance liquid chromatography (UPLC) and demonstrate that UPLC achieves resolution comparable with that of HPLC while requiring orders of magnitude less injection volume and a fraction of the elution time.
26468288	5	13	dep	less	925:928	arg1	fraction					953:960	a fraction	951:960	a fraction of the elution time	951:980	We present a protocol for muropeptide analysis using ultra performance liquid chromatography (UPLC) and demonstrate that UPLC achieves resolution comparable with that of HPLC while requiring orders of magnitude less injection volume and a fraction of the elution time.
26468288	4	14	theme	peptidoglycan	687:699	arg1	composition					701:711	peptidoglycan composition	687:711	peptidoglycan composition	687:711	However, a systematic comparison among strains of a given species has yet to be undertaken, making it difficult to assess the origins of variability in peptidoglycan composition.
26468288	7	15	theme	experimental	1182:1193	arg1	methodology					1213:1223	This combined experimental and computational methodology	1168:1223	This combined experimental and computational methodology	1168:1223	This combined experimental and computational methodology revealed that peptidoglycan composition was approximately maintained across strains from three Gram-negative species despite taxonomical and morphological differences.
26468288	3	16	theme	strand	487:492	arg1	length					494:499	average glycan strand length	472:499	average glycan strand length	472:499	Characteristics such as the degree of cross-linking and average glycan strand length are known to vary across species.
26468288	1	17	theme	short	188:192	arg1	peptidoglycan					204:216	peptidoglycan	204:216	peptidoglycan	204:216	The bacterial cell wall is a network of glycan strands cross-linked by short peptides (peptidoglycan); it is responsible for the mechanical integrity of the cell and shape determination.
26468288	1	17	theme	short	188:192	arg1	peptides					194:201	short peptides	188:201	short peptides (peptidoglycan)	188:217	The bacterial cell wall is a network of glycan strands cross-linked by short peptides (peptidoglycan); it is responsible for the mechanical integrity of the cell and shape determination.
26468288	8	18	theme	Peptidoglycan	1393:1405	arg1	composition					1407:1417	Peptidoglycan composition	1393:1417	Peptidoglycan composition	1393:1417	Peptidoglycan composition and density were maintained after we systematically altered cell size in Escherichia coli using the antibiotic A22, indicating that cell shape is largely decoupled from the biochemistry of peptidoglycan synthesis.
26468288	5	19	theme	liquid	785:790	arg1	UPLC					808:811	UPLC	808:811	UPLC	808:811	We present a protocol for muropeptide analysis using ultra performance liquid chromatography (UPLC) and demonstrate that UPLC achieves resolution comparable with that of HPLC while requiring orders of magnitude less injection volume and a fraction of the elution time.
26468288	5	19	theme	liquid	785:790	arg1	chromatography					792:805	ultra performance liquid chromatography	767:805	ultra performance liquid chromatography (UPLC)	767:812	We present a protocol for muropeptide analysis using ultra performance liquid chromatography (UPLC) and demonstrate that UPLC achieves resolution comparable with that of HPLC while requiring orders of magnitude less injection volume and a fraction of the elution time.
26468288	8	20	theme	synthesis	1622:1630	arg1	biochemistry					1592:1603	the biochemistry	1588:1603	the biochemistry of peptidoglycan synthesis	1588:1630	Peptidoglycan composition and density were maintained after we systematically altered cell size in Escherichia coli using the antibiotic A22, indicating that cell shape is largely decoupled from the biochemistry of peptidoglycan synthesis.
26468288	7	21	theme	combined	1173:1180	arg1	methodology					1213:1223	This combined experimental and computational methodology	1168:1223	This combined experimental and computational methodology	1168:1223	This combined experimental and computational methodology revealed that peptidoglycan composition was approximately maintained across strains from three Gram-negative species despite taxonomical and morphological differences.
26468288	6	22	theme	chromatographic	1074:1088	arg1	peaks					1090:1094	chromatographic peaks	1074:1094	chromatographic peaks	1074:1094	We also developed a software platform to automate the identification and quantification of chromatographic peaks, which we demonstrate has improved accuracy relative to other software.
26468288	2	23	theme	subunits	382:389	arg1	abundance					353:361	the abundance	349:361	the abundance of the muropeptide subunits composing the cell wall	349:413	Liquid chromatography can be used to measure the abundance of the muropeptide subunits composing the cell wall.
26468288	9	24	dep	High-throughput	1633:1647	arg1	sensitive					1650:1658	sensitive	1650:1658	sensitive	1650:1658	High-throughput, sensitive UPLC combined with our automated software for chromatographic analysis will accelerate the discovery of peptidoglycan composition and the molecular mechanisms of cell wall structure determination.
26468288	4	25	theme	systematic	546:555	arg1	comparison					557:566	a systematic comparison	544:566	a systematic comparison among strains of a given species	544:599	However, a systematic comparison among strains of a given species has yet to be undertaken, making it difficult to assess the origins of variability in peptidoglycan composition.
26468288	9	26	theme	molecular	1798:1806	arg1	mechanisms					1808:1817	the molecular mechanisms	1794:1817	the molecular mechanisms of cell wall structure determination	1794:1854	High-throughput, sensitive UPLC combined with our automated software for chromatographic analysis will accelerate the discovery of peptidoglycan composition and the molecular mechanisms of cell wall structure determination.
26468288	7	27	theme	peptidoglycan	1239:1251	arg1	composition					1253:1263	peptidoglycan composition	1239:1263	peptidoglycan composition	1239:1263	This combined experimental and computational methodology revealed that peptidoglycan composition was approximately maintained across strains from three Gram-negative species despite taxonomical and morphological differences.
26468288	5	28	theme	less	925:928	arg1	orders					905:910	orders	905:910	orders of magnitude less injection volume and a fraction of the elution time	905:980	We present a protocol for muropeptide analysis using ultra performance liquid chromatography (UPLC) and demonstrate that UPLC achieves resolution comparable with that of HPLC while requiring orders of magnitude less injection volume and a fraction of the elution time.
26468288	2	29	theme	muropeptide	370:380	arg1	subunits					382:389	the muropeptide subunits	366:389	the muropeptide subunits composing the cell wall	366:413	Liquid chromatography can be used to measure the abundance of the muropeptide subunits composing the cell wall.
26468288	0	30	theme	High-throughput	0:14	arg1	Analyses					34:41	High-throughput, Highly Sensitive Analyses	0:41	High-throughput, Highly Sensitive Analyses of Bacterial Morphogenesis	0:68	High-throughput, Highly Sensitive Analyses of Bacterial Morphogenesis Using Ultra Performance Liquid Chromatography.
26468288	2	31	used	used	333:336	arg2	chromatography					311:324	Liquid chromatography	304:324	Liquid chromatography	304:324	Liquid chromatography can be used to measure the abundance of the muropeptide subunits composing the cell wall.
26468288	2	32	theme	Liquid	304:309	arg1	chromatography					311:324	Liquid chromatography	304:324	Liquid chromatography	304:324	Liquid chromatography can be used to measure the abundance of the muropeptide subunits composing the cell wall.
26468288	1	33	theme	cell	274:277	arg1	integrity					257:265	the mechanical integrity	242:265	the mechanical integrity of the cell and shape determination	242:301	The bacterial cell wall is a network of glycan strands cross-linked by short peptides (peptidoglycan); it is responsible for the mechanical integrity of the cell and shape determination.
26468288	5	34	theme	magnitude	915:923	arg1	orders					905:910	orders	905:910	orders of magnitude less injection volume and a fraction of the elution time	905:980	We present a protocol for muropeptide analysis using ultra performance liquid chromatography (UPLC) and demonstrate that UPLC achieves resolution comparable with that of HPLC while requiring orders of magnitude less injection volume and a fraction of the elution time.
26468288	9	35	theme	automated	1683:1691	arg1	software					1693:1700	our automated software	1679:1700	our automated software for chromatographic analysis	1679:1729	High-throughput, sensitive UPLC combined with our automated software for chromatographic analysis will accelerate the discovery of peptidoglycan composition and the molecular mechanisms of cell wall structure determination.
26468288	3	36	theme	cross-linking	454:466	arg1	degree					444:449	the degree	440:449	the degree of cross-linking and average glycan strand length	440:499	Characteristics such as the degree of cross-linking and average glycan strand length are known to vary across species.
26468288	5	37	theme	injection	930:938	arg1	volume					940:945	injection volume	930:945	injection volume	930:945	We present a protocol for muropeptide analysis using ultra performance liquid chromatography (UPLC) and demonstrate that UPLC achieves resolution comparable with that of HPLC while requiring orders of magnitude less injection volume and a fraction of the elution time.
26468288	9	38	theme	cell	1822:1825	arg1	determination					1842:1854	cell wall structure determination	1822:1854	cell wall structure determination	1822:1854	High-throughput, sensitive UPLC combined with our automated software for chromatographic analysis will accelerate the discovery of peptidoglycan composition and the molecular mechanisms of cell wall structure determination.
26468288	1	39	theme	bacterial	121:129	arg1	wall					136:139	The bacterial cell wall	117:139	The bacterial cell wall	117:139	The bacterial cell wall is a network of glycan strands cross-linked by short peptides (peptidoglycan); it is responsible for the mechanical integrity of the cell and shape determination.
26468288	1	39	theme	bacterial	121:129	arg1	network					146:152	a network	144:152	a network of glycan strands cross-linked by short peptides (peptidoglycan)	144:217	The bacterial cell wall is a network of glycan strands cross-linked by short peptides (peptidoglycan); it is responsible for the mechanical integrity of the cell and shape determination.
26468288	6	40	dep	identification	1037:1050	arg1	the					1033:1035	the	1033:1035	the	1033:1035	We also developed a software platform to automate the identification and quantification of chromatographic peaks, which we demonstrate has improved accuracy relative to other software.
26468288	1	41	theme	shape	283:287	arg1	integrity					257:265	the mechanical integrity	242:265	the mechanical integrity of the cell and shape determination	242:301	The bacterial cell wall is a network of glycan strands cross-linked by short peptides (peptidoglycan); it is responsible for the mechanical integrity of the cell and shape determination.
26468288	9	42	theme	wall	1827:1830	arg1	determination					1842:1854	cell wall structure determination	1822:1854	cell wall structure determination	1822:1854	High-throughput, sensitive UPLC combined with our automated software for chromatographic analysis will accelerate the discovery of peptidoglycan composition and the molecular mechanisms of cell wall structure determination.
26468288	8	43	theme	antibiotic	1519:1528	arg1	A22					1530:1532	the antibiotic A22	1515:1532	the antibiotic A22	1515:1532	Peptidoglycan composition and density were maintained after we systematically altered cell size in Escherichia coli using the antibiotic A22, indicating that cell shape is largely decoupled from the biochemistry of peptidoglycan synthesis.
26468288	8	44	theme	cell	1479:1482	arg1	size					1484:1487	cell size	1479:1487	cell size	1479:1487	Peptidoglycan composition and density were maintained after we systematically altered cell size in Escherichia coli using the antibiotic A22, indicating that cell shape is largely decoupled from the biochemistry of peptidoglycan synthesis.
26468288	2	45	theme	cell	405:408	arg1	wall					410:413	the cell wall	401:413	the cell wall	401:413	Liquid chromatography can be used to measure the abundance of the muropeptide subunits composing the cell wall.
26468288	5	46	theme	ultra	767:771	arg1	UPLC					808:811	UPLC	808:811	UPLC	808:811	We present a protocol for muropeptide analysis using ultra performance liquid chromatography (UPLC) and demonstrate that UPLC achieves resolution comparable with that of HPLC while requiring orders of magnitude less injection volume and a fraction of the elution time.
26468288	5	46	theme	ultra	767:771	arg1	chromatography					792:805	ultra performance liquid chromatography	767:805	ultra performance liquid chromatography (UPLC)	767:812	We present a protocol for muropeptide analysis using ultra performance liquid chromatography (UPLC) and demonstrate that UPLC achieves resolution comparable with that of HPLC while requiring orders of magnitude less injection volume and a fraction of the elution time.
26468288	9	47	theme	structure	1832:1840	arg1	determination					1842:1854	cell wall structure determination	1822:1854	cell wall structure determination	1822:1854	High-throughput, sensitive UPLC combined with our automated software for chromatographic analysis will accelerate the discovery of peptidoglycan composition and the molecular mechanisms of cell wall structure determination.
26468288	7	48	from	species	1334:1340	arg1	strains					1301:1307	strains	1301:1307	strains from three Gram-negative species	1301:1340	This combined experimental and computational methodology revealed that peptidoglycan composition was approximately maintained across strains from three Gram-negative species despite taxonomical and morphological differences.
26468288	0	49	theme	Morphogenesis	56:68	arg1	Analyses					34:41	High-throughput, Highly Sensitive Analyses	0:41	High-throughput, Highly Sensitive Analyses of Bacterial Morphogenesis	0:68	High-throughput, Highly Sensitive Analyses of Bacterial Morphogenesis Using Ultra Performance Liquid Chromatography.
26468288	7	50	theme	computational	1199:1211	arg1	methodology					1213:1223	This combined experimental and computational methodology	1168:1223	This combined experimental and computational methodology	1168:1223	This combined experimental and computational methodology revealed that peptidoglycan composition was approximately maintained across strains from three Gram-negative species despite taxonomical and morphological differences.
26468288	5	51	with	comparable	860:869	arg1	that					876:879	that	876:879	that	876:879	We present a protocol for muropeptide analysis using ultra performance liquid chromatography (UPLC) and demonstrate that UPLC achieves resolution comparable with that of HPLC while requiring orders of magnitude less injection volume and a fraction of the elution time.
26468288	1	52	theme	cell	131:134	arg1	wall					136:139	The bacterial cell wall	117:139	The bacterial cell wall	117:139	The bacterial cell wall is a network of glycan strands cross-linked by short peptides (peptidoglycan); it is responsible for the mechanical integrity of the cell and shape determination.
26468288	1	52	theme	cell	131:134	arg1	network					146:152	a network	144:152	a network of glycan strands cross-linked by short peptides (peptidoglycan)	144:217	The bacterial cell wall is a network of glycan strands cross-linked by short peptides (peptidoglycan); it is responsible for the mechanical integrity of the cell and shape determination.
26468288	0	53	theme	Bacterial	46:54	arg1	Morphogenesis					56:68	Bacterial Morphogenesis	46:68	Bacterial Morphogenesis	46:68	High-throughput, Highly Sensitive Analyses of Bacterial Morphogenesis Using Ultra Performance Liquid Chromatography.
26468288	8	54	theme	cell	1551:1554	arg1	shape					1556:1560	cell shape	1551:1560	cell shape	1551:1560	Peptidoglycan composition and density were maintained after we systematically altered cell size in Escherichia coli using the antibiotic A22, indicating that cell shape is largely decoupled from the biochemistry of peptidoglycan synthesis.
26468288	9	55	theme	chromatographic	1706:1720	arg1	analysis					1722:1729	chromatographic analysis	1706:1729	chromatographic analysis	1706:1729	High-throughput, sensitive UPLC combined with our automated software for chromatographic analysis will accelerate the discovery of peptidoglycan composition and the molecular mechanisms of cell wall structure determination.
26468288	3	56	theme	length	494:499	arg1	degree					444:449	the degree	440:449	the degree of cross-linking and average glycan strand length	440:499	Characteristics such as the degree of cross-linking and average glycan strand length are known to vary across species.
26468288	5	57	theme	elution	969:975	arg1	time					977:980	the elution time	965:980	the elution time	965:980	We present a protocol for muropeptide analysis using ultra performance liquid chromatography (UPLC) and demonstrate that UPLC achieves resolution comparable with that of HPLC while requiring orders of magnitude less injection volume and a fraction of the elution time.
26468288	3	58	theme	average	472:478	arg1	length					494:499	average glycan strand length	472:499	average glycan strand length	472:499	Characteristics such as the degree of cross-linking and average glycan strand length are known to vary across species.
26468288	4	59	theme	species	593:599	arg1	strains					574:580	strains	574:580	strains of a given species	574:599	However, a systematic comparison among strains of a given species has yet to be undertaken, making it difficult to assess the origins of variability in peptidoglycan composition.
26468288	7	60	theme	Gram-negative	1320:1332	arg1	species					1334:1340	three Gram-negative species	1314:1340	three Gram-negative species	1314:1340	This combined experimental and computational methodology revealed that peptidoglycan composition was approximately maintained across strains from three Gram-negative species despite taxonomical and morphological differences.
26468288	5	61	theme	time	977:980	arg1	volume					940:945	injection volume	930:945	injection volume	930:945	We present a protocol for muropeptide analysis using ultra performance liquid chromatography (UPLC) and demonstrate that UPLC achieves resolution comparable with that of HPLC while requiring orders of magnitude less injection volume and a fraction of the elution time.
26468288	5	61	theme	time	977:980	arg1	fraction					953:960	a fraction	951:960	a fraction of the elution time	951:980	We present a protocol for muropeptide analysis using ultra performance liquid chromatography (UPLC) and demonstrate that UPLC achieves resolution comparable with that of HPLC while requiring orders of magnitude less injection volume and a fraction of the elution time.
26468288	3	62	theme	glycan	480:485	arg1	length					494:499	average glycan strand length	472:499	average glycan strand length	472:499	Characteristics such as the degree of cross-linking and average glycan strand length are known to vary across species.
26468288	7	63	theme	morphological	1366:1378	arg1	differences					1380:1390	taxonomical and morphological differences	1350:1390	taxonomical and morphological differences	1350:1390	This combined experimental and computational methodology revealed that peptidoglycan composition was approximately maintained across strains from three Gram-negative species despite taxonomical and morphological differences.
26468288	4	64	theme	given	587:591	arg1	species					593:599	a given species	585:599	a given species	585:599	However, a systematic comparison among strains of a given species has yet to be undertaken, making it difficult to assess the origins of variability in peptidoglycan composition.
26468288	0	65	theme	Ultra	76:80	arg1	Chromatography					101:114	Ultra Performance Liquid Chromatography	76:114	Ultra Performance Liquid Chromatography	76:114	High-throughput, Highly Sensitive Analyses of Bacterial Morphogenesis Using Ultra Performance Liquid Chromatography.
26468288	7	66	theme	taxonomical	1350:1360	arg1	differences					1380:1390	taxonomical and morphological differences	1350:1390	taxonomical and morphological differences	1350:1390	This combined experimental and computational methodology revealed that peptidoglycan composition was approximately maintained across strains from three Gram-negative species despite taxonomical and morphological differences.
26468288	9	67	theme	High-throughput	1633:1647	arg1	UPLC					1660:1663	High-throughput, sensitive UPLC	1633:1663	High-throughput, sensitive UPLC combined with our automated software for chromatographic analysis	1633:1729	High-throughput, sensitive UPLC combined with our automated software for chromatographic analysis will accelerate the discovery of peptidoglycan composition and the molecular mechanisms of cell wall structure determination.
26468288	5	68	theme	performance	773:783	arg1	UPLC					808:811	UPLC	808:811	UPLC	808:811	We present a protocol for muropeptide analysis using ultra performance liquid chromatography (UPLC) and demonstrate that UPLC achieves resolution comparable with that of HPLC while requiring orders of magnitude less injection volume and a fraction of the elution time.
26468288	5	68	theme	performance	773:783	arg1	chromatography					792:805	ultra performance liquid chromatography	767:805	ultra performance liquid chromatography (UPLC)	767:812	We present a protocol for muropeptide analysis using ultra performance liquid chromatography (UPLC) and demonstrate that UPLC achieves resolution comparable with that of HPLC while requiring orders of magnitude less injection volume and a fraction of the elution time.
26468288	0	69	dep	High-throughput	0:14	arg1	Sensitive					24:32	Sensitive	24:32	Sensitive	24:32	High-throughput, Highly Sensitive Analyses of Bacterial Morphogenesis Using Ultra Performance Liquid Chromatography.
26468288	6	70	theme	software	1003:1010	arg1	platform					1012:1019	a software platform	1001:1019	a software platform to automate the identification and quantification of chromatographic peaks, which we demonstrate has improved accuracy relative to other software	1001:1165	We also developed a software platform to automate the identification and quantification of chromatographic peaks, which we demonstrate has improved accuracy relative to other software.
26468288	4	71	theme	variability	672:682	arg1	origins					661:667	the origins	657:667	the origins of variability	657:682	However, a systematic comparison among strains of a given species has yet to be undertaken, making it difficult to assess the origins of variability in peptidoglycan composition.
29352878	7	0	theme	chemical	1046:1053	arg1	structure					1055:1063	its fine chemical structure	1037:1063	its fine chemical structure	1037:1063	Our findings support the view that depending on its fine chemical structure, and likely its insoluble nature, these dietary fibers can be utilized to direct a targeted promotion of the intestinal microbiota to butyrogenic Clostridium cluster XIVa bacteria.
29352878	1	1	theme	fiber	144:148	arg1	structures					172:181	Dietary fiber chemical and physical structures	136:181	Dietary fiber chemical and physical structures	136:181	Dietary fiber chemical and physical structures may be critical to the comprehension of how they may modulate gut bacterial composition.
29352878	7	2	theme	fine	1041:1044	arg1	structure					1055:1063	its fine chemical structure	1037:1063	its fine chemical structure	1037:1063	Our findings support the view that depending on its fine chemical structure, and likely its insoluble nature, these dietary fibers can be utilized to direct a targeted promotion of the intestinal microbiota to butyrogenic Clostridium cluster XIVa bacteria.
29352878	7	3	used	utilized	1127:1134	arg2	fibers					1113:1118	these dietary fibers	1099:1118	these dietary fibers	1099:1118	Our findings support the view that depending on its fine chemical structure, and likely its insoluble nature, these dietary fibers can be utilized to direct a targeted promotion of the intestinal microbiota to butyrogenic Clostridium cluster XIVa bacteria.
29352878	1	4	theme	chemical	150:157	arg1	structures					172:181	Dietary fiber chemical and physical structures	136:181	Dietary fiber chemical and physical structures	136:181	Dietary fiber chemical and physical structures may be critical to the comprehension of how they may modulate gut bacterial composition.
29352878	0	5	theme	Clostridium	92:102	arg1	XIVa					112:115	the butyrogenic Clostridium cluster XIVa	76:115	the butyrogenic Clostridium cluster XIVa	76:115	In vitro fermentation of Cookeina speciosa glucans stimulates the growth of the butyrogenic Clostridium cluster XIVa in a targeted way.
29352878	4	6	dep	in	603:604	arg1	vitro					606:610	vitro	606:610	vitro	606:610	Both glucans were highly butyrogenic and propiogenic, with low gas production, during in vitro fecal fermentation and led to distinct bacterial shifts if compared to fructooligosaccharides.
29352878	1	7	theme	gut	245:247	arg1	composition					259:269	gut bacterial composition	245:269	gut bacterial composition	245:269	Dietary fiber chemical and physical structures may be critical to the comprehension of how they may modulate gut bacterial composition.
29352878	0	8	theme	butyrogenic	80:90	arg1	XIVa					112:115	the butyrogenic Clostridium cluster XIVa	76:115	the butyrogenic Clostridium cluster XIVa	76:115	In vitro fermentation of Cookeina speciosa glucans stimulates the growth of the butyrogenic Clostridium cluster XIVa in a targeted way.
29352878	3	9	theme	-linked	482:488	arg1	β-D-glucans					490:500	a (1→3)-linked β-D-glucans	475:500	a (1→3)-linked β-D-glucans	475:500	Two glucans, characterized as a (1 → 3),(1 → 6)-linked and a (1→3)-linked β-D-glucans were obtained.
29352878	2	10	theme	fermentation	348:359	arg1	profile					361:367	its fermentation profile	344:367	its fermentation profile	344:367	We purified insoluble polymers from Cookeina speciosa, and investigated its fermentation profile in an in vitro human fecal fermentation model.
29352878	1	11	theme	bacterial	249:257	arg1	composition					259:269	gut bacterial composition	245:269	gut bacterial composition	245:269	Dietary fiber chemical and physical structures may be critical to the comprehension of how they may modulate gut bacterial composition.
29352878	0	12	theme	XIVa	112:115	arg1	growth					66:71	the growth	62:71	the growth of the butyrogenic Clostridium cluster XIVa	62:115	In vitro fermentation of Cookeina speciosa glucans stimulates the growth of the butyrogenic Clostridium cluster XIVa in a targeted way.
29352878	6	13	link	-linked	870:876	arg1	β-D-glucan					878:887	The (1 → 3)-linked β-D-glucan	859:887	The (1 → 3)-linked β-D-glucan	859:887	The (1 → 3)-linked β-D-glucan presented a faster fermentation profile compared to the branched (1 → 3),(1 → 6)-linked β-D-glucan.
29352878	2	14	theme	human	384:388	arg1	model					409:413	an in vitro human fecal fermentation model	372:413	an in vitro human fecal fermentation model	372:413	We purified insoluble polymers from Cookeina speciosa, and investigated its fermentation profile in an in vitro human fecal fermentation model.
29352878	0	15	theme	cluster	104:110	arg1	XIVa					112:115	the butyrogenic Clostridium cluster XIVa	76:115	the butyrogenic Clostridium cluster XIVa	76:115	In vitro fermentation of Cookeina speciosa glucans stimulates the growth of the butyrogenic Clostridium cluster XIVa in a targeted way.
29352878	5	16	theme	Bacteroides	729:739	arg1	Anaerostipes					817:828	Anaerostipes	817:828	Anaerostipes	817:828	Specific increases in Bacteroides uniformis and genera from the Clostridium cluster XIVa, such as butyrogenic Anaerostipes and Roseburia were observed.
29352878	5	16	theme	Bacteroides	729:739	arg1	uniformis					741:749	Bacteroides uniformis	729:749	Bacteroides uniformis	729:749	Specific increases in Bacteroides uniformis and genera from the Clostridium cluster XIVa, such as butyrogenic Anaerostipes and Roseburia were observed.
29352878	5	16	theme	Bacteroides	729:739	arg1	Roseburia					834:842	Roseburia	834:842	Roseburia	834:842	Specific increases in Bacteroides uniformis and genera from the Clostridium cluster XIVa, such as butyrogenic Anaerostipes and Roseburia were observed.
29352878	1	17	theme	physical	163:170	arg1	structures					172:181	Dietary fiber chemical and physical structures	136:181	Dietary fiber chemical and physical structures	136:181	Dietary fiber chemical and physical structures may be critical to the comprehension of how they may modulate gut bacterial composition.
29352878	2	18	theme	Cookeina	308:315	arg1	speciosa					317:324	Cookeina speciosa	308:324	Cookeina speciosa	308:324	We purified insoluble polymers from Cookeina speciosa, and investigated its fermentation profile in an in vitro human fecal fermentation model.
29352878	7	19	theme	microbiota	1185:1194	arg1	promotion					1157:1165	a targeted promotion	1146:1165	a targeted promotion of the intestinal microbiota to butyrogenic Clostridium cluster XIVa bacteria	1146:1243	Our findings support the view that depending on its fine chemical structure, and likely its insoluble nature, these dietary fibers can be utilized to direct a targeted promotion of the intestinal microbiota to butyrogenic Clostridium cluster XIVa bacteria.
29352878	2	20	theme	in	375:376	arg1	model					409:413	an in vitro human fecal fermentation model	372:413	an in vitro human fecal fermentation model	372:413	We purified insoluble polymers from Cookeina speciosa, and investigated its fermentation profile in an in vitro human fecal fermentation model.
29352878	0	21	theme	In	0:1	arg1	fermentation					9:20	In vitro fermentation	0:20	In vitro fermentation of Cookeina speciosa glucans	0:49	In vitro fermentation of Cookeina speciosa glucans stimulates the growth of the butyrogenic Clostridium cluster XIVa in a targeted way.
29352878	4	22	theme	in	603:604	arg1	fermentation					618:629	in vitro fecal fermentation	603:629	in vitro fecal fermentation	603:629	Both glucans were highly butyrogenic and propiogenic, with low gas production, during in vitro fecal fermentation and led to distinct bacterial shifts if compared to fructooligosaccharides.
29352878	5	23	theme	Clostridium	771:781	arg1	XIVa					791:794	the Clostridium cluster XIVa	767:794	the Clostridium cluster XIVa	767:794	Specific increases in Bacteroides uniformis and genera from the Clostridium cluster XIVa, such as butyrogenic Anaerostipes and Roseburia were observed.
29352878	4	24	theme	low	576:578	arg1	production					584:593	low gas production	576:593	low gas production	576:593	Both glucans were highly butyrogenic and propiogenic, with low gas production, during in vitro fecal fermentation and led to distinct bacterial shifts if compared to fructooligosaccharides.
29352878	5	25	theme	cluster	783:789	arg1	XIVa					791:794	the Clostridium cluster XIVa	767:794	the Clostridium cluster XIVa	767:794	Specific increases in Bacteroides uniformis and genera from the Clostridium cluster XIVa, such as butyrogenic Anaerostipes and Roseburia were observed.
29352878	3	26	link	-linked	463:469	arg1	glucans					420:426	Two glucans	416:426	Two glucans	416:426	Two glucans, characterized as a (1 → 3),(1 → 6)-linked and a (1→3)-linked β-D-glucans were obtained.
29352878	2	27	theme	insoluble	284:292	arg1	polymers					294:301	insoluble polymers	284:301	insoluble polymers	284:301	We purified insoluble polymers from Cookeina speciosa, and investigated its fermentation profile in an in vitro human fecal fermentation model.
29352878	0	28	theme	speciosa	34:41	arg1	glucans					43:49	Cookeina speciosa glucans	25:49	Cookeina speciosa glucans	25:49	In vitro fermentation of Cookeina speciosa glucans stimulates the growth of the butyrogenic Clostridium cluster XIVa in a targeted way.
29352878	6	29	theme	fermentation	908:919	arg1	profile					921:927	a faster fermentation profile	899:927	a faster fermentation profile	899:927	The (1 → 3)-linked β-D-glucan presented a faster fermentation profile compared to the branched (1 → 3),(1 → 6)-linked β-D-glucan.
29352878	7	30	theme	dietary	1105:1111	arg1	fibers					1113:1118	these dietary fibers	1099:1118	these dietary fibers	1099:1118	Our findings support the view that depending on its fine chemical structure, and likely its insoluble nature, these dietary fibers can be utilized to direct a targeted promotion of the intestinal microbiota to butyrogenic Clostridium cluster XIVa bacteria.
29352878	7	31	theme	Clostridium	1211:1221	arg1	bacteria					1236:1243	butyrogenic Clostridium cluster XIVa bacteria	1199:1243	butyrogenic Clostridium cluster XIVa bacteria	1199:1243	Our findings support the view that depending on its fine chemical structure, and likely its insoluble nature, these dietary fibers can be utilized to direct a targeted promotion of the intestinal microbiota to butyrogenic Clostridium cluster XIVa bacteria.
29352878	7	32	theme	cluster	1223:1229	arg1	bacteria					1236:1243	butyrogenic Clostridium cluster XIVa bacteria	1199:1243	butyrogenic Clostridium cluster XIVa bacteria	1199:1243	Our findings support the view that depending on its fine chemical structure, and likely its insoluble nature, these dietary fibers can be utilized to direct a targeted promotion of the intestinal microbiota to butyrogenic Clostridium cluster XIVa bacteria.
29352878	2	33	theme	fermentation	396:407	arg1	model					409:413	an in vitro human fecal fermentation model	372:413	an in vitro human fecal fermentation model	372:413	We purified insoluble polymers from Cookeina speciosa, and investigated its fermentation profile in an in vitro human fecal fermentation model.
29352878	0	34	theme	Cookeina	25:32	arg1	glucans					43:49	Cookeina speciosa glucans	25:49	Cookeina speciosa glucans	25:49	In vitro fermentation of Cookeina speciosa glucans stimulates the growth of the butyrogenic Clostridium cluster XIVa in a targeted way.
29352878	6	35	theme	faster	901:906	arg1	profile					921:927	a faster fermentation profile	899:927	a faster fermentation profile	899:927	The (1 → 3)-linked β-D-glucan presented a faster fermentation profile compared to the branched (1 → 3),(1 → 6)-linked β-D-glucan.
29352878	0	36	theme	targeted	122:129	arg1	way					131:133	a targeted way	120:133	a targeted way	120:133	In vitro fermentation of Cookeina speciosa glucans stimulates the growth of the butyrogenic Clostridium cluster XIVa in a targeted way.
29352878	7	37	theme	insoluble	1081:1089	arg1	nature					1091:1096	likely its insoluble nature	1070:1096	likely its insoluble nature	1070:1096	Our findings support the view that depending on its fine chemical structure, and likely its insoluble nature, these dietary fibers can be utilized to direct a targeted promotion of the intestinal microbiota to butyrogenic Clostridium cluster XIVa bacteria.
29352878	2	38	theme	fecal	390:394	arg1	model					409:413	an in vitro human fecal fermentation model	372:413	an in vitro human fecal fermentation model	372:413	We purified insoluble polymers from Cookeina speciosa, and investigated its fermentation profile in an in vitro human fecal fermentation model.
29352878	4	39	theme	distinct	642:649	arg1	shifts					661:666	distinct bacterial shifts	642:666	distinct bacterial shifts	642:666	Both glucans were highly butyrogenic and propiogenic, with low gas production, during in vitro fecal fermentation and led to distinct bacterial shifts if compared to fructooligosaccharides.
29352878	0	40	theme	glucans	43:49	arg1	fermentation					9:20	In vitro fermentation	0:20	In vitro fermentation of Cookeina speciosa glucans	0:49	In vitro fermentation of Cookeina speciosa glucans stimulates the growth of the butyrogenic Clostridium cluster XIVa in a targeted way.
29352878	6	41	dep	branched	945:952	arg1	1 → 3					955:959	1 → 3	955:959	1 → 3	955:959	The (1 → 3)-linked β-D-glucan presented a faster fermentation profile compared to the branched (1 → 3),(1 → 6)-linked β-D-glucan.
29352878	6	41	dep	branched	945:952	arg1	-linked					969:975	-linked	969:975	-linked	969:975	The (1 → 3)-linked β-D-glucan presented a faster fermentation profile compared to the branched (1 → 3),(1 → 6)-linked β-D-glucan.
29352878	5	42	from	increases	716:724	arg1	Anaerostipes					817:828	Anaerostipes	817:828	Anaerostipes	817:828	Specific increases in Bacteroides uniformis and genera from the Clostridium cluster XIVa, such as butyrogenic Anaerostipes and Roseburia were observed.
29352878	5	42	from	increases	716:724	arg1	XIVa					791:794	the Clostridium cluster XIVa	767:794	the Clostridium cluster XIVa	767:794	Specific increases in Bacteroides uniformis and genera from the Clostridium cluster XIVa, such as butyrogenic Anaerostipes and Roseburia were observed.
29352878	5	42	from	increases	716:724	arg1	genera					755:760	genera	755:760	genera	755:760	Specific increases in Bacteroides uniformis and genera from the Clostridium cluster XIVa, such as butyrogenic Anaerostipes and Roseburia were observed.
29352878	5	42	from	increases	716:724	arg1	uniformis					741:749	Bacteroides uniformis	729:749	Bacteroides uniformis	729:749	Specific increases in Bacteroides uniformis and genera from the Clostridium cluster XIVa, such as butyrogenic Anaerostipes and Roseburia were observed.
29352878	5	42	from	increases	716:724	arg1	Roseburia					834:842	Roseburia	834:842	Roseburia	834:842	Specific increases in Bacteroides uniformis and genera from the Clostridium cluster XIVa, such as butyrogenic Anaerostipes and Roseburia were observed.
29352878	4	43	with	butyrogenic	542:552	arg1	production					584:593	low gas production	576:593	low gas production	576:593	Both glucans were highly butyrogenic and propiogenic, with low gas production, during in vitro fecal fermentation and led to distinct bacterial shifts if compared to fructooligosaccharides.
29352878	3	44	theme	-linked	463:469	arg1	glucans					420:426	Two glucans	416:426	Two glucans	416:426	Two glucans, characterized as a (1 → 3),(1 → 6)-linked and a (1→3)-linked β-D-glucans were obtained.
29352878	7	45	theme	butyrogenic	1199:1209	arg1	bacteria					1236:1243	butyrogenic Clostridium cluster XIVa bacteria	1199:1243	butyrogenic Clostridium cluster XIVa bacteria	1199:1243	Our findings support the view that depending on its fine chemical structure, and likely its insoluble nature, these dietary fibers can be utilized to direct a targeted promotion of the intestinal microbiota to butyrogenic Clostridium cluster XIVa bacteria.
29352878	2	46	dep	in	375:376	arg1	vitro					378:382	vitro	378:382	vitro	378:382	We purified insoluble polymers from Cookeina speciosa, and investigated its fermentation profile in an in vitro human fecal fermentation model.
29352878	4	47	theme	fecal	612:616	arg1	fermentation					618:629	in vitro fecal fermentation	603:629	in vitro fecal fermentation	603:629	Both glucans were highly butyrogenic and propiogenic, with low gas production, during in vitro fecal fermentation and led to distinct bacterial shifts if compared to fructooligosaccharides.
29352878	7	48	theme	intestinal	1174:1183	arg1	microbiota					1185:1194	the intestinal microbiota	1170:1194	the intestinal microbiota	1170:1194	Our findings support the view that depending on its fine chemical structure, and likely its insoluble nature, these dietary fibers can be utilized to direct a targeted promotion of the intestinal microbiota to butyrogenic Clostridium cluster XIVa bacteria.
29352878	5	49	from	XIVa	791:794	arg1	Anaerostipes					817:828	Anaerostipes	817:828	Anaerostipes	817:828	Specific increases in Bacteroides uniformis and genera from the Clostridium cluster XIVa, such as butyrogenic Anaerostipes and Roseburia were observed.
29352878	5	49	from	XIVa	791:794	arg1	genera					755:760	genera	755:760	genera	755:760	Specific increases in Bacteroides uniformis and genera from the Clostridium cluster XIVa, such as butyrogenic Anaerostipes and Roseburia were observed.
29352878	5	49	from	XIVa	791:794	arg1	increases					716:724	Specific increases	707:724	Specific increases in Bacteroides uniformis and genera from the Clostridium cluster XIVa, such as butyrogenic Anaerostipes and Roseburia	707:842	Specific increases in Bacteroides uniformis and genera from the Clostridium cluster XIVa, such as butyrogenic Anaerostipes and Roseburia were observed.
29352878	5	49	from	XIVa	791:794	arg1	uniformis					741:749	Bacteroides uniformis	729:749	Bacteroides uniformis	729:749	Specific increases in Bacteroides uniformis and genera from the Clostridium cluster XIVa, such as butyrogenic Anaerostipes and Roseburia were observed.
29352878	5	49	from	XIVa	791:794	arg1	Roseburia					834:842	Roseburia	834:842	Roseburia	834:842	Specific increases in Bacteroides uniformis and genera from the Clostridium cluster XIVa, such as butyrogenic Anaerostipes and Roseburia were observed.
29352878	6	50	theme	branched	945:952	arg1	β-D-glucan					977:986	the branched (1 → 3),(1 → 6)-linked β-D-glucan	941:986	the branched (1 → 3),(1 → 6)-linked β-D-glucan	941:986	The (1 → 3)-linked β-D-glucan presented a faster fermentation profile compared to the branched (1 → 3),(1 → 6)-linked β-D-glucan.
29352878	3	51	link	-linked	482:488	arg1	β-D-glucans					490:500	a (1→3)-linked β-D-glucans	475:500	a (1→3)-linked β-D-glucans	475:500	Two glucans, characterized as a (1 → 3),(1 → 6)-linked and a (1→3)-linked β-D-glucans were obtained.
29352878	4	52	theme	bacterial	651:659	arg1	shifts					661:666	distinct bacterial shifts	642:666	distinct bacterial shifts	642:666	Both glucans were highly butyrogenic and propiogenic, with low gas production, during in vitro fecal fermentation and led to distinct bacterial shifts if compared to fructooligosaccharides.
29352878	4	53	theme	gas	580:582	arg1	production					584:593	low gas production	576:593	low gas production	576:593	Both glucans were highly butyrogenic and propiogenic, with low gas production, during in vitro fecal fermentation and led to distinct bacterial shifts if compared to fructooligosaccharides.
29352878	7	54	theme	XIVa	1231:1234	arg1	bacteria					1236:1243	butyrogenic Clostridium cluster XIVa bacteria	1199:1243	butyrogenic Clostridium cluster XIVa bacteria	1199:1243	Our findings support the view that depending on its fine chemical structure, and likely its insoluble nature, these dietary fibers can be utilized to direct a targeted promotion of the intestinal microbiota to butyrogenic Clostridium cluster XIVa bacteria.
29352878	5	55	theme	Specific	707:714	arg1	increases					716:724	Specific increases	707:724	Specific increases in Bacteroides uniformis and genera from the Clostridium cluster XIVa, such as butyrogenic Anaerostipes and Roseburia	707:842	Specific increases in Bacteroides uniformis and genera from the Clostridium cluster XIVa, such as butyrogenic Anaerostipes and Roseburia were observed.
29352878	6	56	theme	-linked	870:876	arg1	β-D-glucan					878:887	The (1 → 3)-linked β-D-glucan	859:887	The (1 → 3)-linked β-D-glucan	859:887	The (1 → 3)-linked β-D-glucan presented a faster fermentation profile compared to the branched (1 → 3),(1 → 6)-linked β-D-glucan.
29352878	0	57	dep	In	0:1	arg1	vitro					3:7	vitro	3:7	vitro	3:7	In vitro fermentation of Cookeina speciosa glucans stimulates the growth of the butyrogenic Clostridium cluster XIVa in a targeted way.
29352878	1	58	theme	Dietary	136:142	arg1	structures					172:181	Dietary fiber chemical and physical structures	136:181	Dietary fiber chemical and physical structures	136:181	Dietary fiber chemical and physical structures may be critical to the comprehension of how they may modulate gut bacterial composition.
29352878	7	59	theme	targeted	1148:1155	arg1	promotion					1157:1165	a targeted promotion	1146:1165	a targeted promotion of the intestinal microbiota to butyrogenic Clostridium cluster XIVa bacteria	1146:1243	Our findings support the view that depending on its fine chemical structure, and likely its insoluble nature, these dietary fibers can be utilized to direct a targeted promotion of the intestinal microbiota to butyrogenic Clostridium cluster XIVa bacteria.
26406097	6	0	theme	good	1007:1010	arg1	properties					1023:1032	relatively good mechanical properties	996:1032	relatively good mechanical properties	996:1032	Furthermore, the use of a porous ionic cross-linked alginate/chitosan composite also has a highly cross-linked structure, which would give the products relatively good mechanical properties.
26406097	5	1	theme	alginate/chitosan	708:724	arg1	composite					726:734	a porous ionic cross-linked alginate/chitosan composite	680:734	a porous ionic cross-linked alginate/chitosan composite	680:734	The use of a porous ionic cross-linked alginate/chitosan composite could provide penetration channels that would lead to the products' increasing penetration rate properties.
26406097	6	2	contain	has	929:931	arg2	properties					1023:1032	relatively good mechanical properties	996:1032	relatively good mechanical properties	996:1032	Furthermore, the use of a porous ionic cross-linked alginate/chitosan composite also has a highly cross-linked structure, which would give the products relatively good mechanical properties.
26406097	6	2	contain	has	929:931	arg1	use					861:863	the use	857:863	the use of a porous ionic cross-linked alginate/chitosan composite	857:922	Furthermore, the use of a porous ionic cross-linked alginate/chitosan composite also has a highly cross-linked structure, which would give the products relatively good mechanical properties.
26406097	5	3	theme	rate	827:830	arg1	properties					832:841	the products' increasing penetration rate properties	790:841	the products' increasing penetration rate properties	790:841	The use of a porous ionic cross-linked alginate/chitosan composite could provide penetration channels that would lead to the products' increasing penetration rate properties.
26406097	7	4	theme	nerve	1133:1137	arg1	regeneration					1139:1150	nerve regeneration	1133:1150	nerve regeneration	1133:1150	Furthermore, the drug could be incorporated into nerve conduits as a new drug-carrying system for nerve regeneration because of its porous and cross-linked structures.
26406097	6	5	link	cross-linked	883:894	arg1	composite					914:922	a porous ionic cross-linked alginate/chitosan composite	868:922	a porous ionic cross-linked alginate/chitosan composite	868:922	Furthermore, the use of a porous ionic cross-linked alginate/chitosan composite also has a highly cross-linked structure, which would give the products relatively good mechanical properties.
26406097	5	6	theme	composite	726:734	arg1	use					673:675	The use	669:675	The use of a porous ionic cross-linked alginate/chitosan composite	669:734	The use of a porous ionic cross-linked alginate/chitosan composite could provide penetration channels that would lead to the products' increasing penetration rate properties.
26406097	6	7	theme	alginate/chitosan	896:912	arg1	composite					914:922	a porous ionic cross-linked alginate/chitosan composite	868:922	a porous ionic cross-linked alginate/chitosan composite	868:922	Furthermore, the use of a porous ionic cross-linked alginate/chitosan composite also has a highly cross-linked structure, which would give the products relatively good mechanical properties.
26406097	3	8	theme	Nerve	403:407	arg1	NCs					419:421	NCs	419:421	NCs	419:421	Nerve conduits (NCs) represent a promising alternative to conventional treatments for peripheral nerve repair.
26406097	3	8	theme	Nerve	403:407	arg1	conduits					409:416	Nerve conduits	403:416	Nerve conduits (NCs)	403:422	Nerve conduits (NCs) represent a promising alternative to conventional treatments for peripheral nerve repair.
26406097	4	9	theme	sodium	562:567	arg1	alginate					569:576	sodium alginate	562:576	sodium alginate	562:576	NCs composed of various polysaccharides such as sodium alginate were designed and prepared by lyophilization as potential matrices for tissue engineering.
26406097	6	10	theme	cross-linked	883:894	arg1	composite					914:922	a porous ionic cross-linked alginate/chitosan composite	868:922	a porous ionic cross-linked alginate/chitosan composite	868:922	Furthermore, the use of a porous ionic cross-linked alginate/chitosan composite also has a highly cross-linked structure, which would give the products relatively good mechanical properties.
26406097	2	11	theme	porous	308:313	arg1	composite					352:360	a porous ionic cross-linked alginate/chitosan composite	306:360	a porous ionic cross-linked alginate/chitosan composite	306:360	New designed nerve conduits with a porous ionic cross-linked alginate/chitosan composite were developed for nervous regeneration.
26406097	0	12	theme	nerve	93:97	arg1	regeneration					99:110	nerve regeneration	93:110	nerve regeneration	93:110	New designed nerve conduits with a porous ionic cross-linked alginate/chitisan structure for nerve regeneration.
26406097	6	13	theme	ionic	877:881	arg1	composite					914:922	a porous ionic cross-linked alginate/chitosan composite	868:922	a porous ionic cross-linked alginate/chitosan composite	868:922	Furthermore, the use of a porous ionic cross-linked alginate/chitosan composite also has a highly cross-linked structure, which would give the products relatively good mechanical properties.
26406097	4	14	theme	tissue	649:654	arg1	engineering					656:666	tissue engineering	649:666	tissue engineering	649:666	NCs composed of various polysaccharides such as sodium alginate were designed and prepared by lyophilization as potential matrices for tissue engineering.
26406097	5	15	theme	penetration	815:825	arg1	properties					832:841	the products' increasing penetration rate properties	790:841	the products' increasing penetration rate properties	790:841	The use of a porous ionic cross-linked alginate/chitosan composite could provide penetration channels that would lead to the products' increasing penetration rate properties.
26406097	1	16	theme	new	115:117	arg1	process					131:137	A new fabrication process	113:137	A new fabrication process for designing nerve conduits with a porous ionic cross-linked alginate/chitosan composite for nervous regeneration	113:252	A new fabrication process for designing nerve conduits with a porous ionic cross-linked alginate/chitosan composite for nervous regeneration could be prepared.
26406097	6	17	theme	porous	870:875	arg1	composite					914:922	a porous ionic cross-linked alginate/chitosan composite	868:922	a porous ionic cross-linked alginate/chitosan composite	868:922	Furthermore, the use of a porous ionic cross-linked alginate/chitosan composite also has a highly cross-linked structure, which would give the products relatively good mechanical properties.
26406097	3	18	theme	conventional	461:472	arg1	treatments					474:483	conventional treatments	461:483	conventional treatments	461:483	Nerve conduits (NCs) represent a promising alternative to conventional treatments for peripheral nerve repair.
26406097	3	19	theme	promising	436:444	arg1	alternative					446:456	a promising alternative	434:456	a promising alternative to conventional treatments for peripheral nerve repair	434:511	Nerve conduits (NCs) represent a promising alternative to conventional treatments for peripheral nerve repair.
26406097	7	20	theme	new	1104:1106	arg1	system					1122:1127	a new drug-carrying system	1102:1127	a new drug-carrying system for nerve regeneration	1102:1150	Furthermore, the drug could be incorporated into nerve conduits as a new drug-carrying system for nerve regeneration because of its porous and cross-linked structures.
26406097	1	21	link	cross-linked	188:199	arg1	composite					219:227	a porous ionic cross-linked alginate/chitosan composite	173:227	a porous ionic cross-linked alginate/chitosan composite for nervous regeneration	173:252	A new fabrication process for designing nerve conduits with a porous ionic cross-linked alginate/chitosan composite for nervous regeneration could be prepared.
26406097	7	22	link	cross-linked	1178:1189	arg1	structures					1191:1200	its porous and cross-linked structures	1163:1200	its porous and cross-linked structures	1163:1200	Furthermore, the drug could be incorporated into nerve conduits as a new drug-carrying system for nerve regeneration because of its porous and cross-linked structures.
26406097	1	23	theme	porous	175:180	arg1	composite					219:227	a porous ionic cross-linked alginate/chitosan composite	173:227	a porous ionic cross-linked alginate/chitosan composite for nervous regeneration	173:252	A new fabrication process for designing nerve conduits with a porous ionic cross-linked alginate/chitosan composite for nervous regeneration could be prepared.
26406097	4	24	theme	various	530:536	arg1	polysaccharides					538:552	various polysaccharides	530:552	various polysaccharides such as sodium alginate	530:576	NCs composed of various polysaccharides such as sodium alginate were designed and prepared by lyophilization as potential matrices for tissue engineering.
26406097	4	24	theme	various	530:536	arg1	alginate					569:576	sodium alginate	562:576	sodium alginate	562:576	NCs composed of various polysaccharides such as sodium alginate were designed and prepared by lyophilization as potential matrices for tissue engineering.
26406097	5	25	theme	ionic	689:693	arg1	composite					726:734	a porous ionic cross-linked alginate/chitosan composite	680:734	a porous ionic cross-linked alginate/chitosan composite	680:734	The use of a porous ionic cross-linked alginate/chitosan composite could provide penetration channels that would lead to the products' increasing penetration rate properties.
26406097	1	26	theme	ionic	182:186	arg1	composite					219:227	a porous ionic cross-linked alginate/chitosan composite	173:227	a porous ionic cross-linked alginate/chitosan composite for nervous regeneration	173:252	A new fabrication process for designing nerve conduits with a porous ionic cross-linked alginate/chitosan composite for nervous regeneration could be prepared.
26406097	5	27	theme	cross-linked	695:706	arg1	composite					726:734	a porous ionic cross-linked alginate/chitosan composite	680:734	a porous ionic cross-linked alginate/chitosan composite	680:734	The use of a porous ionic cross-linked alginate/chitosan composite could provide penetration channels that would lead to the products' increasing penetration rate properties.
26406097	2	28	theme	alginate/chitosan	334:350	arg1	composite					352:360	a porous ionic cross-linked alginate/chitosan composite	306:360	a porous ionic cross-linked alginate/chitosan composite	306:360	New designed nerve conduits with a porous ionic cross-linked alginate/chitosan composite were developed for nervous regeneration.
26406097	1	29	theme	cross-linked	188:199	arg1	composite					219:227	a porous ionic cross-linked alginate/chitosan composite	173:227	a porous ionic cross-linked alginate/chitosan composite for nervous regeneration	173:252	A new fabrication process for designing nerve conduits with a porous ionic cross-linked alginate/chitosan composite for nervous regeneration could be prepared.
26406097	2	30	theme	cross-linked	321:332	arg1	composite					352:360	a porous ionic cross-linked alginate/chitosan composite	306:360	a porous ionic cross-linked alginate/chitosan composite	306:360	New designed nerve conduits with a porous ionic cross-linked alginate/chitosan composite were developed for nervous regeneration.
26406097	1	31	theme	alginate/chitosan	201:217	arg1	composite					219:227	a porous ionic cross-linked alginate/chitosan composite	173:227	a porous ionic cross-linked alginate/chitosan composite for nervous regeneration	173:252	A new fabrication process for designing nerve conduits with a porous ionic cross-linked alginate/chitosan composite for nervous regeneration could be prepared.
26406097	0	32	theme	nerve	13:17	arg1	conduits					19:26	nerve conduits	13:26	nerve conduits with a porous ionic cross-linked alginate/chitisan structure for nerve regeneration	13:110	New designed nerve conduits with a porous ionic cross-linked alginate/chitisan structure for nerve regeneration.
26406097	4	33	theme	potential	626:634	arg1	matrices					636:643	potential matrices	626:643	potential matrices for tissue engineering	626:666	NCs composed of various polysaccharides such as sodium alginate were designed and prepared by lyophilization as potential matrices for tissue engineering.
26406097	4	33	theme	potential	626:634	arg1	NCs					514:516	NCs	514:516	NCs composed of various polysaccharides such as sodium alginate	514:576	NCs composed of various polysaccharides such as sodium alginate were designed and prepared by lyophilization as potential matrices for tissue engineering.
26406097	7	34	theme	porous	1167:1172	arg1	structures					1191:1200	its porous and cross-linked structures	1163:1200	its porous and cross-linked structures	1163:1200	Furthermore, the drug could be incorporated into nerve conduits as a new drug-carrying system for nerve regeneration because of its porous and cross-linked structures.
26406097	2	35	theme	ionic	315:319	arg1	composite					352:360	a porous ionic cross-linked alginate/chitosan composite	306:360	a porous ionic cross-linked alginate/chitosan composite	306:360	New designed nerve conduits with a porous ionic cross-linked alginate/chitosan composite were developed for nervous regeneration.
26406097	7	36	theme	cross-linked	1178:1189	arg1	structures					1191:1200	its porous and cross-linked structures	1163:1200	its porous and cross-linked structures	1163:1200	Furthermore, the drug could be incorporated into nerve conduits as a new drug-carrying system for nerve regeneration because of its porous and cross-linked structures.
26406097	3	37	theme	nerve	500:504	arg1	repair					506:511	peripheral nerve repair	489:511	peripheral nerve repair	489:511	Nerve conduits (NCs) represent a promising alternative to conventional treatments for peripheral nerve repair.
26406097	2	38	link	cross-linked	321:332	arg1	composite					352:360	a porous ionic cross-linked alginate/chitosan composite	306:360	a porous ionic cross-linked alginate/chitosan composite	306:360	New designed nerve conduits with a porous ionic cross-linked alginate/chitosan composite were developed for nervous regeneration.
26406097	6	39	theme	cross-linked	942:953	arg1	structure					955:963	a highly cross-linked structure	933:963	a highly cross-linked structure	933:963	Furthermore, the use of a porous ionic cross-linked alginate/chitosan composite also has a highly cross-linked structure, which would give the products relatively good mechanical properties.
26406097	5	40	link	cross-linked	695:706	arg1	composite					726:734	a porous ionic cross-linked alginate/chitosan composite	680:734	a porous ionic cross-linked alginate/chitosan composite	680:734	The use of a porous ionic cross-linked alginate/chitosan composite could provide penetration channels that would lead to the products' increasing penetration rate properties.
26406097	0	41	with	conduits	19:26	arg1	structure					79:87	a porous ionic cross-linked alginate/chitisan structure	33:87	a porous ionic cross-linked alginate/chitisan structure	33:87	New designed nerve conduits with a porous ionic cross-linked alginate/chitisan structure for nerve regeneration.
26406097	1	42	theme	nervous	233:239	arg1	regeneration					241:252	nervous regeneration	233:252	nervous regeneration	233:252	A new fabrication process for designing nerve conduits with a porous ionic cross-linked alginate/chitosan composite for nervous regeneration could be prepared.
26406097	0	43	theme	ionic	42:46	arg1	structure					79:87	a porous ionic cross-linked alginate/chitisan structure	33:87	a porous ionic cross-linked alginate/chitisan structure	33:87	New designed nerve conduits with a porous ionic cross-linked alginate/chitisan structure for nerve regeneration.
26406097	5	44	theme	penetration	750:760	arg1	channels					762:769	penetration channels	750:769	penetration channels that would lead to the products' increasing penetration rate properties	750:841	The use of a porous ionic cross-linked alginate/chitosan composite could provide penetration channels that would lead to the products' increasing penetration rate properties.
26406097	7	45	theme	drug-carrying	1108:1120	arg1	system					1122:1127	a new drug-carrying system	1102:1127	a new drug-carrying system for nerve regeneration	1102:1150	Furthermore, the drug could be incorporated into nerve conduits as a new drug-carrying system for nerve regeneration because of its porous and cross-linked structures.
26406097	2	46	theme	nervous	381:387	arg1	regeneration					389:400	nervous regeneration	381:400	nervous regeneration	381:400	New designed nerve conduits with a porous ionic cross-linked alginate/chitosan composite were developed for nervous regeneration.
26406097	0	47	theme	porous	35:40	arg1	structure					79:87	a porous ionic cross-linked alginate/chitisan structure	33:87	a porous ionic cross-linked alginate/chitisan structure	33:87	New designed nerve conduits with a porous ionic cross-linked alginate/chitisan structure for nerve regeneration.
26406097	5	48	theme	porous	682:687	arg1	composite					726:734	a porous ionic cross-linked alginate/chitosan composite	680:734	a porous ionic cross-linked alginate/chitosan composite	680:734	The use of a porous ionic cross-linked alginate/chitosan composite could provide penetration channels that would lead to the products' increasing penetration rate properties.
26406097	1	49	theme	fabrication	119:129	arg1	process					131:137	A new fabrication process	113:137	A new fabrication process for designing nerve conduits with a porous ionic cross-linked alginate/chitosan composite for nervous regeneration	113:252	A new fabrication process for designing nerve conduits with a porous ionic cross-linked alginate/chitosan composite for nervous regeneration could be prepared.
26406097	2	50	theme	nerve	286:290	arg1	conduits					292:299	New designed nerve conduits	273:299	New designed nerve conduits with a porous ionic cross-linked alginate/chitosan composite	273:360	New designed nerve conduits with a porous ionic cross-linked alginate/chitosan composite were developed for nervous regeneration.
26406097	0	51	link	cross-linked	48:59	arg1	structure					79:87	a porous ionic cross-linked alginate/chitisan structure	33:87	a porous ionic cross-linked alginate/chitisan structure	33:87	New designed nerve conduits with a porous ionic cross-linked alginate/chitisan structure for nerve regeneration.
26406097	2	52	theme	designed	277:284	arg1	conduits					292:299	New designed nerve conduits	273:299	New designed nerve conduits with a porous ionic cross-linked alginate/chitosan composite	273:360	New designed nerve conduits with a porous ionic cross-linked alginate/chitosan composite were developed for nervous regeneration.
26406097	7	53	theme	nerve	1084:1088	arg1	conduits					1090:1097	nerve conduits	1084:1097	nerve conduits as a new drug-carrying system for nerve regeneration	1084:1150	Furthermore, the drug could be incorporated into nerve conduits as a new drug-carrying system for nerve regeneration because of its porous and cross-linked structures.
26406097	2	54	with	conduits	292:299	arg1	composite					352:360	a porous ionic cross-linked alginate/chitosan composite	306:360	a porous ionic cross-linked alginate/chitosan composite	306:360	New designed nerve conduits with a porous ionic cross-linked alginate/chitosan composite were developed for nervous regeneration.
26406097	0	55	theme	alginate/chitisan	61:77	arg1	structure					79:87	a porous ionic cross-linked alginate/chitisan structure	33:87	a porous ionic cross-linked alginate/chitisan structure	33:87	New designed nerve conduits with a porous ionic cross-linked alginate/chitisan structure for nerve regeneration.
26406097	6	56	theme	composite	914:922	arg1	use					861:863	the use	857:863	the use of a porous ionic cross-linked alginate/chitosan composite	857:922	Furthermore, the use of a porous ionic cross-linked alginate/chitosan composite also has a highly cross-linked structure, which would give the products relatively good mechanical properties.
26406097	2	57	theme	New	273:275	arg1	conduits					292:299	New designed nerve conduits	273:299	New designed nerve conduits with a porous ionic cross-linked alginate/chitosan composite	273:360	New designed nerve conduits with a porous ionic cross-linked alginate/chitosan composite were developed for nervous regeneration.
26406097	3	58	theme	peripheral	489:498	arg1	repair					506:511	peripheral nerve repair	489:511	peripheral nerve repair	489:511	Nerve conduits (NCs) represent a promising alternative to conventional treatments for peripheral nerve repair.
26406097	6	59	link	cross-linked	942:953	arg1	structure					955:963	a highly cross-linked structure	933:963	a highly cross-linked structure	933:963	Furthermore, the use of a porous ionic cross-linked alginate/chitosan composite also has a highly cross-linked structure, which would give the products relatively good mechanical properties.
26406097	0	60	theme	cross-linked	48:59	arg1	structure					79:87	a porous ionic cross-linked alginate/chitisan structure	33:87	a porous ionic cross-linked alginate/chitisan structure	33:87	New designed nerve conduits with a porous ionic cross-linked alginate/chitisan structure for nerve regeneration.
26406097	6	61	theme	mechanical	1012:1021	arg1	properties					1023:1032	relatively good mechanical properties	996:1032	relatively good mechanical properties	996:1032	Furthermore, the use of a porous ionic cross-linked alginate/chitosan composite also has a highly cross-linked structure, which would give the products relatively good mechanical properties.
26406097	1	62	theme	nerve	153:157	arg1	conduits					159:166	nerve conduits	153:166	nerve conduits	153:166	A new fabrication process for designing nerve conduits with a porous ionic cross-linked alginate/chitosan composite for nervous regeneration could be prepared.
26406097	5	63	theme	increasing	804:813	arg1	properties					832:841	the products' increasing penetration rate properties	790:841	the products' increasing penetration rate properties	790:841	The use of a porous ionic cross-linked alginate/chitosan composite could provide penetration channels that would lead to the products' increasing penetration rate properties.
26256377	2	0	theme	bio-based	522:530	arg1	materials					542:550	new bio-based composite materials	518:550	new bio-based composite materials	518:550	The chemical composition, morphology, thermal properties and degree of crystallinity of the celluloses were characterized to discuss their suitability for new bio-based composite materials.
26256377	1	1	theme	liquefaction	170:181	arg1	combination					144:154	the combination	140:154	the combination of bleach and liquefaction processes	140:191	A cellulose extraction from banana pseudo-stem through the combination of bleach and liquefaction processes was described: holocellulose was isolated by the sodium chlorite method, and then the cellulose was obtained by removing the hemicellulose through chemical liquefaction.
26256377	6	2	theme	enhanced	1029:1036	arg1	stability					1046:1054	enhanced thermal stability	1029:1054	enhanced thermal stability	1029:1054	Thermal analysis results show that the cellulose exhibit enhanced thermal stability over holocellulose.
26256377	2	3	theme	new	518:520	arg1	materials					542:550	new bio-based composite materials	518:550	new bio-based composite materials	518:550	The chemical composition, morphology, thermal properties and degree of crystallinity of the celluloses were characterized to discuss their suitability for new bio-based composite materials.
26256377	5	4	theme	individual	824:833	arg1	fibrils					835:841	individual fibrils	824:841	individual fibrils	824:841	Surface morphological studies revealed that there is a reduction in fiber diameter and fiber bundles are divided into individual fibrils, which lead to an increase of the surface area and results in an increased adhesion at the fiber-matrix interface in composites.
26256377	4	5	theme	hemicellulose	636:648	arg1	content					650:656	The hemicellulose content	632:656	The hemicellulose content in cellulose	632:669	The hemicellulose content in cellulose is decreased from 45.69% to 8.38%.
26256377	7	6	theme	81.26	1148:1152	arg1	%					1143:1143	%	1143:1143	%	1143:1143	XRD results indicate that the crystallinity is increased from 52.22% to 81.26% by liquefaction.
26256377	5	7	theme	increased	908:916	arg1	adhesion					918:925	an increased adhesion	905:925	an increased adhesion at the fiber-matrix interface in composites	905:969	Surface morphological studies revealed that there is a reduction in fiber diameter and fiber bundles are divided into individual fibrils, which lead to an increase of the surface area and results in an increased adhesion at the fiber-matrix interface in composites.
26256377	5	8	theme	surface	877:883	arg1	area					885:888	the surface area	873:888	the surface area	873:888	Surface morphological studies revealed that there is a reduction in fiber diameter and fiber bundles are divided into individual fibrils, which lead to an increase of the surface area and results in an increased adhesion at the fiber-matrix interface in composites.
26256377	5	9	theme	fiber	774:778	arg1	diameter					780:787	fiber diameter	774:787	fiber diameter	774:787	Surface morphological studies revealed that there is a reduction in fiber diameter and fiber bundles are divided into individual fibrils, which lead to an increase of the surface area and results in an increased adhesion at the fiber-matrix interface in composites.
26256377	7	10	theme	52.22	1138:1142	arg1	%					1143:1143	%	1143:1143	%	1143:1143	XRD results indicate that the crystallinity is increased from 52.22% to 81.26% by liquefaction.
26256377	4	11	theme	8.38	699:702	arg1	%					694:694	%	694:694	%	694:694	The hemicellulose content in cellulose is decreased from 45.69% to 8.38%.
26256377	0	12	from	pseudo-stem	42:52	arg1	Characterization					0:15	Characterization	0:15	Characterization of cellulose from banana pseudo-stem by heterogeneous liquefaction.	0:83	Characterization of cellulose from banana pseudo-stem by heterogeneous liquefaction.
26256377	1	13	theme	cellulose	87:95	arg1	extraction					97:106	A cellulose extraction	85:106	A cellulose extraction from banana pseudo-stem through the combination of bleach and liquefaction processes	85:191	A cellulose extraction from banana pseudo-stem through the combination of bleach and liquefaction processes was described: holocellulose was isolated by the sodium chlorite method, and then the cellulose was obtained by removing the hemicellulose through chemical liquefaction.
26256377	5	14	theme	area	885:888	arg1	increase					861:868	an increase	858:868	an increase of the surface area	858:888	Surface morphological studies revealed that there is a reduction in fiber diameter and fiber bundles are divided into individual fibrils, which lead to an increase of the surface area and results in an increased adhesion at the fiber-matrix interface in composites.
26256377	4	15	from	content	650:656	arg1	cellulose					661:669	cellulose	661:669	cellulose	661:669	The hemicellulose content in cellulose is decreased from 45.69% to 8.38%.
26256377	5	16	from	interface	947:955	arg1	adhesion					918:925	an increased adhesion	905:925	an increased adhesion at the fiber-matrix interface in composites	905:969	Surface morphological studies revealed that there is a reduction in fiber diameter and fiber bundles are divided into individual fibrils, which lead to an increase of the surface area and results in an increased adhesion at the fiber-matrix interface in composites.
26256377	5	16	from	interface	947:955	arg1	composites					960:969	composites	960:969	composites	960:969	Surface morphological studies revealed that there is a reduction in fiber diameter and fiber bundles are divided into individual fibrils, which lead to an increase of the surface area and results in an increased adhesion at the fiber-matrix interface in composites.
26256377	2	17	theme	thermal	401:407	arg1	properties					409:418	thermal properties	401:418	thermal properties	401:418	The chemical composition, morphology, thermal properties and degree of crystallinity of the celluloses were characterized to discuss their suitability for new bio-based composite materials.
26256377	1	18	dep	bleach	159:164	arg1	processes					183:191	processes	183:191	processes	183:191	A cellulose extraction from banana pseudo-stem through the combination of bleach and liquefaction processes was described: holocellulose was isolated by the sodium chlorite method, and then the cellulose was obtained by removing the hemicellulose through chemical liquefaction.
26256377	2	19	theme	celluloses	455:464	arg1	crystallinity					434:446	crystallinity	434:446	crystallinity of the celluloses	434:464	The chemical composition, morphology, thermal properties and degree of crystallinity of the celluloses were characterized to discuss their suitability for new bio-based composite materials.
26256377	6	20	theme	analysis	980:987	arg1	results					989:995	Thermal analysis results	972:995	Thermal analysis results	972:995	Thermal analysis results show that the cellulose exhibit enhanced thermal stability over holocellulose.
26256377	4	21	dep	%	694:694	arg1	to					696:697	to	696:697	to	696:697	The hemicellulose content in cellulose is decreased from 45.69% to 8.38%.
26256377	1	22	theme	banana	113:118	arg1	pseudo-stem					120:130	banana pseudo-stem	113:130	banana pseudo-stem	113:130	A cellulose extraction from banana pseudo-stem through the combination of bleach and liquefaction processes was described: holocellulose was isolated by the sodium chlorite method, and then the cellulose was obtained by removing the hemicellulose through chemical liquefaction.
26256377	2	23	theme	crystallinity	434:446	arg1	degree					424:429	degree	424:429	degree	424:429	The chemical composition, morphology, thermal properties and degree of crystallinity of the celluloses were characterized to discuss their suitability for new bio-based composite materials.
26256377	2	23	theme	crystallinity	434:446	arg1	morphology					389:398	morphology	389:398	morphology	389:398	The chemical composition, morphology, thermal properties and degree of crystallinity of the celluloses were characterized to discuss their suitability for new bio-based composite materials.
26256377	2	23	theme	crystallinity	434:446	arg1	properties					409:418	thermal properties	401:418	thermal properties	401:418	The chemical composition, morphology, thermal properties and degree of crystallinity of the celluloses were characterized to discuss their suitability for new bio-based composite materials.
26256377	2	23	theme	crystallinity	434:446	arg1	composition					376:386	The chemical composition	363:386	The chemical composition	363:386	The chemical composition, morphology, thermal properties and degree of crystallinity of the celluloses were characterized to discuss their suitability for new bio-based composite materials.
26256377	0	24	theme	banana	35:40	arg1	pseudo-stem					42:52	banana pseudo-stem	35:52	banana pseudo-stem	35:52	Characterization of cellulose from banana pseudo-stem by heterogeneous liquefaction.
26256377	6	25	theme	Thermal	972:978	arg1	results					989:995	Thermal analysis results	972:995	Thermal analysis results	972:995	Thermal analysis results show that the cellulose exhibit enhanced thermal stability over holocellulose.
26256377	5	26	from	reduction	761:769	arg1	diameter					780:787	fiber diameter	774:787	fiber diameter	774:787	Surface morphological studies revealed that there is a reduction in fiber diameter and fiber bundles are divided into individual fibrils, which lead to an increase of the surface area and results in an increased adhesion at the fiber-matrix interface in composites.
26256377	4	27	theme	45.69	689:693	arg1	%					694:694	%	694:694	%	694:694	The hemicellulose content in cellulose is decreased from 45.69% to 8.38%.
26256377	0	28	theme	heterogeneous	57:69	arg1	liquefaction					71:82	heterogeneous liquefaction	57:82	heterogeneous liquefaction	57:82	Characterization of cellulose from banana pseudo-stem by heterogeneous liquefaction.
26256377	5	29	theme	Surface	706:712	arg1	studies					728:734	Surface morphological studies	706:734	Surface morphological studies	706:734	Surface morphological studies revealed that there is a reduction in fiber diameter and fiber bundles are divided into individual fibrils, which lead to an increase of the surface area and results in an increased adhesion at the fiber-matrix interface in composites.
26256377	5	30	theme	fiber	793:797	arg1	bundles					799:805	fiber bundles	793:805	fiber bundles	793:805	Surface morphological studies revealed that there is a reduction in fiber diameter and fiber bundles are divided into individual fibrils, which lead to an increase of the surface area and results in an increased adhesion at the fiber-matrix interface in composites.
26256377	2	31	theme	chemical	367:374	arg1	composition					376:386	The chemical composition	363:386	The chemical composition	363:386	The chemical composition, morphology, thermal properties and degree of crystallinity of the celluloses were characterized to discuss their suitability for new bio-based composite materials.
26256377	7	32	dep	%	1143:1143	arg1	to					1145:1146	to	1145:1146	to	1145:1146	XRD results indicate that the crystallinity is increased from 52.22% to 81.26% by liquefaction.
26256377	5	33	theme	morphological	714:726	arg1	studies					728:734	Surface morphological studies	706:734	Surface morphological studies	706:734	Surface morphological studies revealed that there is a reduction in fiber diameter and fiber bundles are divided into individual fibrils, which lead to an increase of the surface area and results in an increased adhesion at the fiber-matrix interface in composites.
26256377	1	34	theme	chemical	340:347	arg1	liquefaction					349:360	chemical liquefaction	340:360	chemical liquefaction	340:360	A cellulose extraction from banana pseudo-stem through the combination of bleach and liquefaction processes was described: holocellulose was isolated by the sodium chlorite method, and then the cellulose was obtained by removing the hemicellulose through chemical liquefaction.
26256377	5	35	theme	fiber-matrix	934:945	arg1	interface					947:955	the fiber-matrix interface	930:955	the fiber-matrix interface in composites	930:969	Surface morphological studies revealed that there is a reduction in fiber diameter and fiber bundles are divided into individual fibrils, which lead to an increase of the surface area and results in an increased adhesion at the fiber-matrix interface in composites.
26256377	1	36	from	pseudo-stem	120:130	arg1	extraction					97:106	A cellulose extraction	85:106	A cellulose extraction from banana pseudo-stem through the combination of bleach and liquefaction processes	85:191	A cellulose extraction from banana pseudo-stem through the combination of bleach and liquefaction processes was described: holocellulose was isolated by the sodium chlorite method, and then the cellulose was obtained by removing the hemicellulose through chemical liquefaction.
26256377	1	37	theme	sodium	242:247	arg1	method					258:263	the sodium chlorite method	238:263	the sodium chlorite method	238:263	A cellulose extraction from banana pseudo-stem through the combination of bleach and liquefaction processes was described: holocellulose was isolated by the sodium chlorite method, and then the cellulose was obtained by removing the hemicellulose through chemical liquefaction.
26256377	7	38	theme	XRD	1076:1078	arg1	results					1080:1086	XRD results	1076:1086	XRD results	1076:1086	XRD results indicate that the crystallinity is increased from 52.22% to 81.26% by liquefaction.
26256377	1	39	theme	chlorite	249:256	arg1	method					258:263	the sodium chlorite method	238:263	the sodium chlorite method	238:263	A cellulose extraction from banana pseudo-stem through the combination of bleach and liquefaction processes was described: holocellulose was isolated by the sodium chlorite method, and then the cellulose was obtained by removing the hemicellulose through chemical liquefaction.
26256377	1	40	theme	bleach	159:164	arg1	combination					144:154	the combination	140:154	the combination of bleach and liquefaction processes	140:191	A cellulose extraction from banana pseudo-stem through the combination of bleach and liquefaction processes was described: holocellulose was isolated by the sodium chlorite method, and then the cellulose was obtained by removing the hemicellulose through chemical liquefaction.
26256377	2	41	theme	composite	532:540	arg1	materials					542:550	new bio-based composite materials	518:550	new bio-based composite materials	518:550	The chemical composition, morphology, thermal properties and degree of crystallinity of the celluloses were characterized to discuss their suitability for new bio-based composite materials.
26256377	0	42	theme	cellulose	20:28	arg1	Characterization					0:15	Characterization	0:15	Characterization of cellulose from banana pseudo-stem by heterogeneous liquefaction.	0:83	Characterization of cellulose from banana pseudo-stem by heterogeneous liquefaction.
26256377	6	43	theme	thermal	1038:1044	arg1	stability					1046:1054	enhanced thermal stability	1029:1054	enhanced thermal stability	1029:1054	Thermal analysis results show that the cellulose exhibit enhanced thermal stability over holocellulose.
25911738	2	0	theme	native	313:318	arg1	samples					350:356	both native and pre-treated plant biomass samples	308:356	both native and pre-treated plant biomass samples	308:356	Studies on plant cell walls can be conducted on both native and pre-treated plant biomass samples, allowing an enhanced understanding of these structural and compositional variations.
25911738	6	1	from	materials	1235:1243	arg1	loosening					1140:1148	loosening	1140:1148	loosening	1140:1148	In general, irrespective of the phylogenetic origin, AFEX™ pre-treatment appeared to cause loosening and improved accessibility of various xylan epitope subclasses in most plant biomass materials studied.
25911738	6	1	from	materials	1235:1243	arg1	accessibility					1163:1175	improved accessibility	1154:1175	improved accessibility	1154:1175	In general, irrespective of the phylogenetic origin, AFEX™ pre-treatment appeared to cause loosening and improved accessibility of various xylan epitope subclasses in most plant biomass materials studied.
25911738	9	2	from	composition	1904:1914	arg1	understanding					1859:1871	an improved understanding	1847:1871	an improved understanding of variations in structure and composition of plant cell walls	1847:1934	It was found that monitoring changes in cell wall glycan compositions and their relative extractability for untreated and pre-treated plant biomass can provide an improved understanding of variations in structure and composition of plant cell walls and delineate the role(s) of matrix polysaccharides in cell wall recalcitrance.
25911738	9	3	theme	monitoring	1705:1714	arg1	changes					1716:1722	monitoring changes	1705:1722	monitoring changes in cell wall glycan compositions and their relative extractability for untreated and pre-treated plant biomass	1705:1833	It was found that monitoring changes in cell wall glycan compositions and their relative extractability for untreated and pre-treated plant biomass can provide an improved understanding of variations in structure and composition of plant cell walls and delineate the role(s) of matrix polysaccharides in cell wall recalcitrance.
25911738	9	4	from	structure	1890:1898	arg1	understanding					1859:1871	an improved understanding	1847:1871	an improved understanding of variations in structure and composition of plant cell walls	1847:1934	It was found that monitoring changes in cell wall glycan compositions and their relative extractability for untreated and pre-treated plant biomass can provide an improved understanding of variations in structure and composition of plant cell walls and delineate the role(s) of matrix polysaccharides in cell wall recalcitrance.
25911738	6	5	theme	improved	1154:1161	arg1	accessibility					1163:1175	improved accessibility	1154:1175	improved accessibility	1154:1175	In general, irrespective of the phylogenetic origin, AFEX™ pre-treatment appeared to cause loosening and improved accessibility of various xylan epitope subclasses in most plant biomass materials studied.
25911738	3	6	theme	matrix	519:524	arg1	polysaccharides					526:540	matrix polysaccharides	519:540	matrix polysaccharides	519:540	Here glycome profiling was employed to determine the relative abundance of matrix polysaccharides in several phylogenetically distinct native and pre-treated plant biomasses.
25911738	4	7	dep	AFEX™	794:798	arg1	expansion					815:823	ammonia fiber expansion	801:823	ammonia fiber expansion	801:823	Eight distinct biomass types belonging to four different subgroups (i.e. monocot grasses, woody dicots, herbaceous dicots, and softwoods) were subjected to various regimes of AFEX™ (ammonia fiber expansion) pre-treatment [AFEX is a trademark of MBI, Lansing (http://www.mbi.org].
25911738	9	8	from	variations	1876:1885	arg1	composition					1904:1914	composition	1904:1914	composition	1904:1914	It was found that monitoring changes in cell wall glycan compositions and their relative extractability for untreated and pre-treated plant biomass can provide an improved understanding of variations in structure and composition of plant cell walls and delineate the role(s) of matrix polysaccharides in cell wall recalcitrance.
25911738	9	8	from	variations	1876:1885	arg1	structure					1890:1898	structure	1890:1898	structure	1890:1898	It was found that monitoring changes in cell wall glycan compositions and their relative extractability for untreated and pre-treated plant biomass can provide an improved understanding of variations in structure and composition of plant cell walls and delineate the role(s) of matrix polysaccharides in cell wall recalcitrance.
25911738	9	9	theme	polysaccharides	1972:1986	arg1	role					1954:1957	the role	1950:1957	the role(s) of matrix polysaccharides in cell wall recalcitrance	1950:2013	It was found that monitoring changes in cell wall glycan compositions and their relative extractability for untreated and pre-treated plant biomass can provide an improved understanding of variations in structure and composition of plant cell walls and delineate the role(s) of matrix polysaccharides in cell wall recalcitrance.
25911738	9	9	theme	polysaccharides	1972:1986	arg1	s					1959:1959	s	1959:1959	s	1959:1959	It was found that monitoring changes in cell wall glycan compositions and their relative extractability for untreated and pre-treated plant biomass can provide an improved understanding of variations in structure and composition of plant cell walls and delineate the role(s) of matrix polysaccharides in cell wall recalcitrance.
25911738	3	10	theme	native	579:584	arg1	biomasses					608:616	several phylogenetically distinct native and pre-treated plant biomasses	545:616	several phylogenetically distinct native and pre-treated plant biomasses	545:616	Here glycome profiling was employed to determine the relative abundance of matrix polysaccharides in several phylogenetically distinct native and pre-treated plant biomasses.
25911738	4	11	dep	regimes	783:789	arg1	[AFEX					840:844	AFEX™ (ammonia fiber expansion) pre-treatment [AFEX	794:844	AFEX™ (ammonia fiber expansion) pre-treatment [AFEX	794:844	Eight distinct biomass types belonging to four different subgroups (i.e. monocot grasses, woody dicots, herbaceous dicots, and softwoods) were subjected to various regimes of AFEX™ (ammonia fiber expansion) pre-treatment [AFEX is a trademark of MBI, Lansing (http://www.mbi.org].
25911738	4	11	dep	regimes	783:789	arg1	trademark					851:859	a trademark	849:859	a trademark of MBI, Lansing (http://www.mbi.org]	849:896	Eight distinct biomass types belonging to four different subgroups (i.e. monocot grasses, woody dicots, herbaceous dicots, and softwoods) were subjected to various regimes of AFEX™ (ammonia fiber expansion) pre-treatment [AFEX is a trademark of MBI, Lansing (http://www.mbi.org].
25911738	7	12	theme	xyloglucan	1396:1405	arg1	epitopes					1407:1414	pectin and xyloglucan epitopes	1385:1414	pectin and xyloglucan epitopes	1385:1414	For most biomass types analysed, such loosening was also evident for other major non-cellulosic components including subclasses of pectin and xyloglucan epitopes.
25911738	9	13	theme	cell	1991:1994	arg1	recalcitrance					2001:2013	cell wall recalcitrance	1991:2013	cell wall recalcitrance	1991:2013	It was found that monitoring changes in cell wall glycan compositions and their relative extractability for untreated and pre-treated plant biomass can provide an improved understanding of variations in structure and composition of plant cell walls and delineate the role(s) of matrix polysaccharides in cell wall recalcitrance.
25911738	2	14	theme	plant	271:275	arg1	walls					282:286	plant cell walls	271:286	plant cell walls	271:286	Studies on plant cell walls can be conducted on both native and pre-treated plant biomass samples, allowing an enhanced understanding of these structural and compositional variations.
25911738	1	15	theme	biomass	182:188	arg1	bulk					168:171	the bulk	164:171	the bulk of plant biomass	164:188	Cell walls, which constitute the bulk of plant biomass, vary considerably in their structure, composition, and architecture.
25911738	8	16	theme	AFEX™	1451:1455	arg1	pre-treatment					1457:1469	AFEX™ pre-treatment	1451:1469	AFEX™ pre-treatment	1451:1469	The studies also demonstrate that AFEX™ pre-treatment significantly reduced cell wall recalcitrance among diverse phylogenies (except softwoods) by inducing structural modifications to polysaccharides that were not detectable by conventional gross composition analyses.
25911738	6	17	theme	biomass	1227:1233	arg1	materials					1235:1243	most plant biomass materials	1216:1243	most plant biomass materials	1216:1243	In general, irrespective of the phylogenetic origin, AFEX™ pre-treatment appeared to cause loosening and improved accessibility of various xylan epitope subclasses in most plant biomass materials studied.
25911738	0	18	theme	biomass	126:132	arg1	profiling					83:91	glycome profiling	75:91	glycome profiling of native and AFEXTM-pre-treated biomass	75:132	Insights into plant cell wall structure, architecture, and integrity using glycome profiling of native and AFEXTM-pre-treated biomass.
25911738	9	19	from	changes	1716:1722	arg1	wall					1732:1735	cell wall glycan compositions and their relative extractability for untreated and pre-treated plant biomass	1727:1833	wall	1732:1735	It was found that monitoring changes in cell wall glycan compositions and their relative extractability for untreated and pre-treated plant biomass can provide an improved understanding of variations in structure and composition of plant cell walls and delineate the role(s) of matrix polysaccharides in cell wall recalcitrance.
25911738	9	19	from	changes	1716:1722	arg1	extractability					1776:1789	their relative extractability	1761:1789	their relative extractability for untreated and pre-treated plant biomass	1761:1833	It was found that monitoring changes in cell wall glycan compositions and their relative extractability for untreated and pre-treated plant biomass can provide an improved understanding of variations in structure and composition of plant cell walls and delineate the role(s) of matrix polysaccharides in cell wall recalcitrance.
25911738	8	20	theme	structural	1574:1583	arg1	modifications					1585:1597	structural modifications	1574:1597	structural modifications to polysaccharides that were not detectable by conventional gross composition analyses	1574:1684	The studies also demonstrate that AFEX™ pre-treatment significantly reduced cell wall recalcitrance among diverse phylogenies (except softwoods) by inducing structural modifications to polysaccharides that were not detectable by conventional gross composition analyses.
25911738	6	21	theme	most	1216:1219	arg1	materials					1235:1243	most plant biomass materials	1216:1243	most plant biomass materials	1216:1243	In general, irrespective of the phylogenetic origin, AFEX™ pre-treatment appeared to cause loosening and improved accessibility of various xylan epitope subclasses in most plant biomass materials studied.
25911738	3	22	theme	pre-treated	590:600	arg1	biomasses					608:616	several phylogenetically distinct native and pre-treated plant biomasses	545:616	several phylogenetically distinct native and pre-treated plant biomasses	545:616	Here glycome profiling was employed to determine the relative abundance of matrix polysaccharides in several phylogenetically distinct native and pre-treated plant biomasses.
25911738	9	23	theme	untreated	1795:1803	arg1	biomass					1827:1833	untreated and pre-treated plant biomass	1795:1833	untreated and pre-treated plant biomass	1795:1833	It was found that monitoring changes in cell wall glycan compositions and their relative extractability for untreated and pre-treated plant biomass can provide an improved understanding of variations in structure and composition of plant cell walls and delineate the role(s) of matrix polysaccharides in cell wall recalcitrance.
25911738	8	24	theme	cell	1493:1496	arg1	recalcitrance					1503:1515	cell wall recalcitrance	1493:1515	cell wall recalcitrance among diverse phylogenies (except softwoods)	1493:1560	The studies also demonstrate that AFEX™ pre-treatment significantly reduced cell wall recalcitrance among diverse phylogenies (except softwoods) by inducing structural modifications to polysaccharides that were not detectable by conventional gross composition analyses.
25911738	6	25	theme	subclasses	1202:1211	arg1	loosening					1140:1148	loosening	1140:1148	loosening	1140:1148	In general, irrespective of the phylogenetic origin, AFEX™ pre-treatment appeared to cause loosening and improved accessibility of various xylan epitope subclasses in most plant biomass materials studied.
25911738	6	25	theme	subclasses	1202:1211	arg1	accessibility					1163:1175	improved accessibility	1154:1175	improved accessibility	1154:1175	In general, irrespective of the phylogenetic origin, AFEX™ pre-treatment appeared to cause loosening and improved accessibility of various xylan epitope subclasses in most plant biomass materials studied.
25911738	5	26	theme	high-throughput	1023:1037	arg1	platform					1039:1046	a high-throughput platform	1021:1046	a high-throughput platform	1021:1046	This approach allowed detailed analysis of close to 200 cell wall glycan epitopes and their relative extractability using a high-throughput platform.
25911738	9	27	theme	pre-treated	1809:1819	arg1	biomass					1827:1833	untreated and pre-treated plant biomass	1795:1833	untreated and pre-treated plant biomass	1795:1833	It was found that monitoring changes in cell wall glycan compositions and their relative extractability for untreated and pre-treated plant biomass can provide an improved understanding of variations in structure and composition of plant cell walls and delineate the role(s) of matrix polysaccharides in cell wall recalcitrance.
25911738	7	28	theme	major	1329:1333	arg1	components					1350:1359	other major non-cellulosic components	1323:1359	other major non-cellulosic components including subclasses of pectin and xyloglucan epitopes	1323:1414	For most biomass types analysed, such loosening was also evident for other major non-cellulosic components including subclasses of pectin and xyloglucan epitopes.
25911738	7	28	theme	major	1329:1333	arg1	subclasses					1371:1380	subclasses	1371:1380	subclasses of pectin and xyloglucan epitopes	1371:1414	For most biomass types analysed, such loosening was also evident for other major non-cellulosic components including subclasses of pectin and xyloglucan epitopes.
25911738	4	29	dep	http	878:881	arg1	//www.mbi.org					883:895	//www.mbi.org	883:895	http://www.mbi.org	878:895	Eight distinct biomass types belonging to four different subgroups (i.e. monocot grasses, woody dicots, herbaceous dicots, and softwoods) were subjected to various regimes of AFEX™ (ammonia fiber expansion) pre-treatment [AFEX is a trademark of MBI, Lansing (http://www.mbi.org].
25911738	8	30	theme	diverse	1523:1529	arg1	phylogenies					1531:1541	diverse phylogenies	1523:1541	diverse phylogenies (except softwoods)	1523:1560	The studies also demonstrate that AFEX™ pre-treatment significantly reduced cell wall recalcitrance among diverse phylogenies (except softwoods) by inducing structural modifications to polysaccharides that were not detectable by conventional gross composition analyses.
25911738	4	31	theme	woody	709:713	arg1	dicots					715:720	woody dicots	709:720	woody dicots	709:720	Eight distinct biomass types belonging to four different subgroups (i.e. monocot grasses, woody dicots, herbaceous dicots, and softwoods) were subjected to various regimes of AFEX™ (ammonia fiber expansion) pre-treatment [AFEX is a trademark of MBI, Lansing (http://www.mbi.org].
25911738	4	32	dep	Lansing	869:875	arg1	http					878:881	http	878:881	http://www.mbi.org	878:895	Eight distinct biomass types belonging to four different subgroups (i.e. monocot grasses, woody dicots, herbaceous dicots, and softwoods) were subjected to various regimes of AFEX™ (ammonia fiber expansion) pre-treatment [AFEX is a trademark of MBI, Lansing (http://www.mbi.org].
25911738	0	33	theme	glycome	75:81	arg1	profiling					83:91	glycome profiling	75:91	glycome profiling of native and AFEXTM-pre-treated biomass	75:132	Insights into plant cell wall structure, architecture, and integrity using glycome profiling of native and AFEXTM-pre-treated biomass.
25911738	9	34	theme	improved	1850:1857	arg1	understanding					1859:1871	an improved understanding	1847:1871	an improved understanding of variations in structure and composition of plant cell walls	1847:1934	It was found that monitoring changes in cell wall glycan compositions and their relative extractability for untreated and pre-treated plant biomass can provide an improved understanding of variations in structure and composition of plant cell walls and delineate the role(s) of matrix polysaccharides in cell wall recalcitrance.
25911738	4	35	theme	various	775:781	arg1	regimes					783:789	various regimes	775:789	various regimes of AFEX™ (ammonia fiber expansion) pre-treatment [AFEX is a trademark of MBI, Lansing (http://www.mbi.org]	775:896	Eight distinct biomass types belonging to four different subgroups (i.e. monocot grasses, woody dicots, herbaceous dicots, and softwoods) were subjected to various regimes of AFEX™ (ammonia fiber expansion) pre-treatment [AFEX is a trademark of MBI, Lansing (http://www.mbi.org].
25911738	7	36	theme	other	1323:1327	arg1	components					1350:1359	other major non-cellulosic components	1323:1359	other major non-cellulosic components including subclasses of pectin and xyloglucan epitopes	1323:1414	For most biomass types analysed, such loosening was also evident for other major non-cellulosic components including subclasses of pectin and xyloglucan epitopes.
25911738	7	36	theme	other	1323:1327	arg1	subclasses					1371:1380	subclasses	1371:1380	subclasses of pectin and xyloglucan epitopes	1371:1414	For most biomass types analysed, such loosening was also evident for other major non-cellulosic components including subclasses of pectin and xyloglucan epitopes.
25911738	2	37	theme	compositional	418:430	arg1	variations					432:441	these structural and compositional variations	397:441	these structural and compositional variations	397:441	Studies on plant cell walls can be conducted on both native and pre-treated plant biomass samples, allowing an enhanced understanding of these structural and compositional variations.
25911738	6	38	from	accessibility	1163:1175	arg1	materials					1235:1243	most plant biomass materials	1216:1243	most plant biomass materials	1216:1243	In general, irrespective of the phylogenetic origin, AFEX™ pre-treatment appeared to cause loosening and improved accessibility of various xylan epitope subclasses in most plant biomass materials studied.
25911738	2	39	theme	structural	403:412	arg1	variations					432:441	these structural and compositional variations	397:441	these structural and compositional variations	397:441	Studies on plant cell walls can be conducted on both native and pre-treated plant biomass samples, allowing an enhanced understanding of these structural and compositional variations.
25911738	6	40	theme	xylan	1188:1192	arg1	subclasses					1202:1211	various xylan epitope subclasses	1180:1211	various xylan epitope subclasses in most plant biomass materials studied	1180:1251	In general, irrespective of the phylogenetic origin, AFEX™ pre-treatment appeared to cause loosening and improved accessibility of various xylan epitope subclasses in most plant biomass materials studied.
25911738	0	41	theme	plant	14:18	arg1	structure					30:38	plant cell wall structure	14:38	plant cell wall structure	14:38	Insights into plant cell wall structure, architecture, and integrity using glycome profiling of native and AFEXTM-pre-treated biomass.
25911738	4	42	theme	fiber	809:813	arg1	expansion					815:823	ammonia fiber expansion	801:823	ammonia fiber expansion	801:823	Eight distinct biomass types belonging to four different subgroups (i.e. monocot grasses, woody dicots, herbaceous dicots, and softwoods) were subjected to various regimes of AFEX™ (ammonia fiber expansion) pre-treatment [AFEX is a trademark of MBI, Lansing (http://www.mbi.org].
25911738	2	43	theme	enhanced	371:378	arg1	understanding					380:392	an enhanced understanding	368:392	an enhanced understanding of these structural and compositional variations	368:441	Studies on plant cell walls can be conducted on both native and pre-treated plant biomass samples, allowing an enhanced understanding of these structural and compositional variations.
25911738	0	44	theme	wall	25:28	arg1	structure					30:38	plant cell wall structure	14:38	plant cell wall structure	14:38	Insights into plant cell wall structure, architecture, and integrity using glycome profiling of native and AFEXTM-pre-treated biomass.
25911738	7	45	theme	such	1287:1290	arg1	loosening					1292:1300	such loosening	1287:1300	such loosening	1287:1300	For most biomass types analysed, such loosening was also evident for other major non-cellulosic components including subclasses of pectin and xyloglucan epitopes.
25911738	9	46	theme	cell	1727:1730	arg1	wall					1732:1735	cell wall glycan compositions and their relative extractability for untreated and pre-treated plant biomass	1727:1833	wall	1732:1735	It was found that monitoring changes in cell wall glycan compositions and their relative extractability for untreated and pre-treated plant biomass can provide an improved understanding of variations in structure and composition of plant cell walls and delineate the role(s) of matrix polysaccharides in cell wall recalcitrance.
25911738	6	47	theme	AFEX™	1102:1106	arg1	pre-treatment					1108:1120	AFEX™ pre-treatment	1102:1120	AFEX™ pre-treatment	1102:1120	In general, irrespective of the phylogenetic origin, AFEX™ pre-treatment appeared to cause loosening and improved accessibility of various xylan epitope subclasses in most plant biomass materials studied.
25911738	9	48	theme	cell	1925:1928	arg1	walls					1930:1934	plant cell walls	1919:1934	plant cell walls	1919:1934	It was found that monitoring changes in cell wall glycan compositions and their relative extractability for untreated and pre-treated plant biomass can provide an improved understanding of variations in structure and composition of plant cell walls and delineate the role(s) of matrix polysaccharides in cell wall recalcitrance.
25911738	6	49	theme	various	1180:1186	arg1	subclasses					1202:1211	various xylan epitope subclasses	1180:1211	various xylan epitope subclasses in most plant biomass materials studied	1180:1251	In general, irrespective of the phylogenetic origin, AFEX™ pre-treatment appeared to cause loosening and improved accessibility of various xylan epitope subclasses in most plant biomass materials studied.
25911738	7	50	theme	biomass	1263:1269	arg1	types					1271:1275	most biomass types	1258:1275	most biomass types analysed	1258:1284	For most biomass types analysed, such loosening was also evident for other major non-cellulosic components including subclasses of pectin and xyloglucan epitopes.
25911738	4	51	theme	biomass	634:640	arg1	types					642:646	Eight distinct biomass types	619:646	Eight distinct biomass types belonging to four different subgroups (i.e. monocot grasses, woody dicots, herbaceous dicots, and softwoods)	619:755	Eight distinct biomass types belonging to four different subgroups (i.e. monocot grasses, woody dicots, herbaceous dicots, and softwoods) were subjected to various regimes of AFEX™ (ammonia fiber expansion) pre-treatment [AFEX is a trademark of MBI, Lansing (http://www.mbi.org].
25911738	3	52	theme	relative	497:504	arg1	abundance					506:514	the relative abundance	493:514	the relative abundance of matrix polysaccharides in several phylogenetically distinct native and pre-treated plant biomasses	493:616	Here glycome profiling was employed to determine the relative abundance of matrix polysaccharides in several phylogenetically distinct native and pre-treated plant biomasses.
25911738	5	53	theme	epitopes	972:979	arg1	analysis					930:937	detailed analysis	921:937	detailed analysis of close to 200 cell wall glycan epitopes	921:979	This approach allowed detailed analysis of close to 200 cell wall glycan epitopes and their relative extractability using a high-throughput platform.
25911738	5	53	theme	epitopes	972:979	arg1	extractability					1000:1013	their relative extractability	985:1013	their relative extractability using a high-throughput platform	985:1046	This approach allowed detailed analysis of close to 200 cell wall glycan epitopes and their relative extractability using a high-throughput platform.
25911738	8	54	theme	gross	1659:1663	arg1	analyses					1677:1684	conventional gross composition analyses	1646:1684	conventional gross composition analyses	1646:1684	The studies also demonstrate that AFEX™ pre-treatment significantly reduced cell wall recalcitrance among diverse phylogenies (except softwoods) by inducing structural modifications to polysaccharides that were not detectable by conventional gross composition analyses.
25911738	3	55	theme	polysaccharides	526:540	arg1	abundance					506:514	the relative abundance	493:514	the relative abundance of matrix polysaccharides in several phylogenetically distinct native and pre-treated plant biomasses	493:616	Here glycome profiling was employed to determine the relative abundance of matrix polysaccharides in several phylogenetically distinct native and pre-treated plant biomasses.
25911738	4	56	theme	MBI	864:866	arg1	[AFEX					840:844	AFEX™ (ammonia fiber expansion) pre-treatment [AFEX	794:844	AFEX™ (ammonia fiber expansion) pre-treatment [AFEX	794:844	Eight distinct biomass types belonging to four different subgroups (i.e. monocot grasses, woody dicots, herbaceous dicots, and softwoods) were subjected to various regimes of AFEX™ (ammonia fiber expansion) pre-treatment [AFEX is a trademark of MBI, Lansing (http://www.mbi.org].
25911738	4	56	theme	MBI	864:866	arg1	trademark					851:859	a trademark	849:859	a trademark of MBI, Lansing (http://www.mbi.org]	849:896	Eight distinct biomass types belonging to four different subgroups (i.e. monocot grasses, woody dicots, herbaceous dicots, and softwoods) were subjected to various regimes of AFEX™ (ammonia fiber expansion) pre-treatment [AFEX is a trademark of MBI, Lansing (http://www.mbi.org].
25911738	3	57	from	abundance	506:514	arg1	biomasses					608:616	several phylogenetically distinct native and pre-treated plant biomasses	545:616	several phylogenetically distinct native and pre-treated plant biomasses	545:616	Here glycome profiling was employed to determine the relative abundance of matrix polysaccharides in several phylogenetically distinct native and pre-treated plant biomasses.
25911738	2	58	theme	variations	432:441	arg1	understanding					380:392	an enhanced understanding	368:392	an enhanced understanding of these structural and compositional variations	368:441	Studies on plant cell walls can be conducted on both native and pre-treated plant biomass samples, allowing an enhanced understanding of these structural and compositional variations.
25911738	4	59	theme	pre-treatment	826:838	arg1	[AFEX					840:844	AFEX™ (ammonia fiber expansion) pre-treatment [AFEX	794:844	AFEX™ (ammonia fiber expansion) pre-treatment [AFEX	794:844	Eight distinct biomass types belonging to four different subgroups (i.e. monocot grasses, woody dicots, herbaceous dicots, and softwoods) were subjected to various regimes of AFEX™ (ammonia fiber expansion) pre-treatment [AFEX is a trademark of MBI, Lansing (http://www.mbi.org].
25911738	4	59	theme	pre-treatment	826:838	arg1	trademark					851:859	a trademark	849:859	a trademark of MBI, Lansing (http://www.mbi.org]	849:896	Eight distinct biomass types belonging to four different subgroups (i.e. monocot grasses, woody dicots, herbaceous dicots, and softwoods) were subjected to various regimes of AFEX™ (ammonia fiber expansion) pre-treatment [AFEX is a trademark of MBI, Lansing (http://www.mbi.org].
25911738	9	60	from	role	1954:1957	arg1	recalcitrance					2001:2013	cell wall recalcitrance	1991:2013	cell wall recalcitrance	1991:2013	It was found that monitoring changes in cell wall glycan compositions and their relative extractability for untreated and pre-treated plant biomass can provide an improved understanding of variations in structure and composition of plant cell walls and delineate the role(s) of matrix polysaccharides in cell wall recalcitrance.
25911738	9	61	theme	matrix	1965:1970	arg1	polysaccharides					1972:1986	matrix polysaccharides	1965:1986	matrix polysaccharides	1965:1986	It was found that monitoring changes in cell wall glycan compositions and their relative extractability for untreated and pre-treated plant biomass can provide an improved understanding of variations in structure and composition of plant cell walls and delineate the role(s) of matrix polysaccharides in cell wall recalcitrance.
25911738	4	62	theme	different	666:674	arg1	subgroups					676:684	four different subgroups	661:684	four different subgroups (i.e. monocot grasses, woody dicots, herbaceous dicots, and softwoods)	661:755	Eight distinct biomass types belonging to four different subgroups (i.e. monocot grasses, woody dicots, herbaceous dicots, and softwoods) were subjected to various regimes of AFEX™ (ammonia fiber expansion) pre-treatment [AFEX is a trademark of MBI, Lansing (http://www.mbi.org].
25911738	3	63	theme	distinct	570:577	arg1	biomasses					608:616	several phylogenetically distinct native and pre-treated plant biomasses	545:616	several phylogenetically distinct native and pre-treated plant biomasses	545:616	Here glycome profiling was employed to determine the relative abundance of matrix polysaccharides in several phylogenetically distinct native and pre-treated plant biomasses.
25911738	9	64	theme	glycan	1737:1742	arg1	compositions					1744:1755	glycan compositions	1737:1755	glycan compositions	1737:1755	It was found that monitoring changes in cell wall glycan compositions and their relative extractability for untreated and pre-treated plant biomass can provide an improved understanding of variations in structure and composition of plant cell walls and delineate the role(s) of matrix polysaccharides in cell wall recalcitrance.
25911738	0	65	theme	AFEXTM-pre-treated	107:124	arg1	biomass					126:132	native and AFEXTM-pre-treated biomass	96:132	native and AFEXTM-pre-treated biomass	96:132	Insights into plant cell wall structure, architecture, and integrity using glycome profiling of native and AFEXTM-pre-treated biomass.
25911738	2	66	theme	cell	277:280	arg1	walls					282:286	plant cell walls	271:286	plant cell walls	271:286	Studies on plant cell walls can be conducted on both native and pre-treated plant biomass samples, allowing an enhanced understanding of these structural and compositional variations.
25911738	1	67	theme	plant	176:180	arg1	biomass					182:188	plant biomass	176:188	plant biomass	176:188	Cell walls, which constitute the bulk of plant biomass, vary considerably in their structure, composition, and architecture.
25911738	2	68	theme	plant	336:340	arg1	samples					350:356	both native and pre-treated plant biomass samples	308:356	both native and pre-treated plant biomass samples	308:356	Studies on plant cell walls can be conducted on both native and pre-treated plant biomass samples, allowing an enhanced understanding of these structural and compositional variations.
25911738	7	69	theme	epitopes	1407:1414	arg1	subclasses					1371:1380	subclasses	1371:1380	subclasses of pectin and xyloglucan epitopes	1371:1414	For most biomass types analysed, such loosening was also evident for other major non-cellulosic components including subclasses of pectin and xyloglucan epitopes.
25911738	9	70	theme	wall	1996:1999	arg1	recalcitrance					2001:2013	cell wall recalcitrance	1991:2013	cell wall recalcitrance	1991:2013	It was found that monitoring changes in cell wall glycan compositions and their relative extractability for untreated and pre-treated plant biomass can provide an improved understanding of variations in structure and composition of plant cell walls and delineate the role(s) of matrix polysaccharides in cell wall recalcitrance.
25911738	3	71	theme	plant	602:606	arg1	biomasses					608:616	several phylogenetically distinct native and pre-treated plant biomasses	545:616	several phylogenetically distinct native and pre-treated plant biomasses	545:616	Here glycome profiling was employed to determine the relative abundance of matrix polysaccharides in several phylogenetically distinct native and pre-treated plant biomasses.
25911738	9	72	theme	relative	1767:1774	arg1	extractability					1776:1789	their relative extractability	1761:1789	their relative extractability for untreated and pre-treated plant biomass	1761:1833	It was found that monitoring changes in cell wall glycan compositions and their relative extractability for untreated and pre-treated plant biomass can provide an improved understanding of variations in structure and composition of plant cell walls and delineate the role(s) of matrix polysaccharides in cell wall recalcitrance.
25911738	6	73	theme	plant	1221:1225	arg1	materials					1235:1243	most plant biomass materials	1216:1243	most plant biomass materials	1216:1243	In general, irrespective of the phylogenetic origin, AFEX™ pre-treatment appeared to cause loosening and improved accessibility of various xylan epitope subclasses in most plant biomass materials studied.
25911738	8	74	theme	wall	1498:1501	arg1	recalcitrance					1503:1515	cell wall recalcitrance	1493:1515	cell wall recalcitrance among diverse phylogenies (except softwoods)	1493:1560	The studies also demonstrate that AFEX™ pre-treatment significantly reduced cell wall recalcitrance among diverse phylogenies (except softwoods) by inducing structural modifications to polysaccharides that were not detectable by conventional gross composition analyses.
25911738	6	75	theme	epitope	1194:1200	arg1	subclasses					1202:1211	various xylan epitope subclasses	1180:1211	various xylan epitope subclasses in most plant biomass materials studied	1180:1251	In general, irrespective of the phylogenetic origin, AFEX™ pre-treatment appeared to cause loosening and improved accessibility of various xylan epitope subclasses in most plant biomass materials studied.
25911738	4	76	theme	herbaceous	723:732	arg1	dicots					734:739	herbaceous dicots	723:739	herbaceous dicots	723:739	Eight distinct biomass types belonging to four different subgroups (i.e. monocot grasses, woody dicots, herbaceous dicots, and softwoods) were subjected to various regimes of AFEX™ (ammonia fiber expansion) pre-treatment [AFEX is a trademark of MBI, Lansing (http://www.mbi.org].
25911738	1	77	theme	Cell	135:138	arg1	walls					140:144	Cell walls	135:144	Cell walls	135:144	Cell walls, which constitute the bulk of plant biomass, vary considerably in their structure, composition, and architecture.
25911738	2	78	from	Studies	260:266	arg1	walls					282:286	plant cell walls	271:286	plant cell walls	271:286	Studies on plant cell walls can be conducted on both native and pre-treated plant biomass samples, allowing an enhanced understanding of these structural and compositional variations.
25911738	9	79	theme	plant	1821:1825	arg1	biomass					1827:1833	untreated and pre-treated plant biomass	1795:1833	untreated and pre-treated plant biomass	1795:1833	It was found that monitoring changes in cell wall glycan compositions and their relative extractability for untreated and pre-treated plant biomass can provide an improved understanding of variations in structure and composition of plant cell walls and delineate the role(s) of matrix polysaccharides in cell wall recalcitrance.
25911738	7	80	theme	non-cellulosic	1335:1348	arg1	components					1350:1359	other major non-cellulosic components	1323:1359	other major non-cellulosic components including subclasses of pectin and xyloglucan epitopes	1323:1414	For most biomass types analysed, such loosening was also evident for other major non-cellulosic components including subclasses of pectin and xyloglucan epitopes.
25911738	7	80	theme	non-cellulosic	1335:1348	arg1	subclasses					1371:1380	subclasses	1371:1380	subclasses of pectin and xyloglucan epitopes	1371:1414	For most biomass types analysed, such loosening was also evident for other major non-cellulosic components including subclasses of pectin and xyloglucan epitopes.
25911738	4	81	theme	monocot	692:698	arg1	grasses					700:706	monocot grasses	692:706	monocot grasses	692:706	Eight distinct biomass types belonging to four different subgroups (i.e. monocot grasses, woody dicots, herbaceous dicots, and softwoods) were subjected to various regimes of AFEX™ (ammonia fiber expansion) pre-treatment [AFEX is a trademark of MBI, Lansing (http://www.mbi.org].
25911738	6	82	from	subclasses	1202:1211	arg1	materials					1235:1243	most plant biomass materials	1216:1243	most plant biomass materials	1216:1243	In general, irrespective of the phylogenetic origin, AFEX™ pre-treatment appeared to cause loosening and improved accessibility of various xylan epitope subclasses in most plant biomass materials studied.
25911738	4	83	theme	distinct	625:632	arg1	types					642:646	Eight distinct biomass types	619:646	Eight distinct biomass types belonging to four different subgroups (i.e. monocot grasses, woody dicots, herbaceous dicots, and softwoods)	619:755	Eight distinct biomass types belonging to four different subgroups (i.e. monocot grasses, woody dicots, herbaceous dicots, and softwoods) were subjected to various regimes of AFEX™ (ammonia fiber expansion) pre-treatment [AFEX is a trademark of MBI, Lansing (http://www.mbi.org].
25911738	0	84	theme	native	96:101	arg1	biomass					126:132	native and AFEXTM-pre-treated biomass	96:132	native and AFEXTM-pre-treated biomass	96:132	Insights into plant cell wall structure, architecture, and integrity using glycome profiling of native and AFEXTM-pre-treated biomass.
25911738	4	85	dep	grasses	700:706	arg1	i.e.					687:690	i.e.	687:690	i.e.	687:690	Eight distinct biomass types belonging to four different subgroups (i.e. monocot grasses, woody dicots, herbaceous dicots, and softwoods) were subjected to various regimes of AFEX™ (ammonia fiber expansion) pre-treatment [AFEX is a trademark of MBI, Lansing (http://www.mbi.org].
25911738	9	86	theme	variations	1876:1885	arg1	understanding					1859:1871	an improved understanding	1847:1871	an improved understanding of variations in structure and composition of plant cell walls	1847:1934	It was found that monitoring changes in cell wall glycan compositions and their relative extractability for untreated and pre-treated plant biomass can provide an improved understanding of variations in structure and composition of plant cell walls and delineate the role(s) of matrix polysaccharides in cell wall recalcitrance.
25911738	2	87	theme	biomass	342:348	arg1	samples					350:356	both native and pre-treated plant biomass samples	308:356	both native and pre-treated plant biomass samples	308:356	Studies on plant cell walls can be conducted on both native and pre-treated plant biomass samples, allowing an enhanced understanding of these structural and compositional variations.
25911738	0	88	theme	cell	20:23	arg1	structure					30:38	plant cell wall structure	14:38	plant cell wall structure	14:38	Insights into plant cell wall structure, architecture, and integrity using glycome profiling of native and AFEXTM-pre-treated biomass.
25911738	4	89	dep	subgroups	676:684	arg1	softwoods					746:754	softwoods	746:754	softwoods	746:754	Eight distinct biomass types belonging to four different subgroups (i.e. monocot grasses, woody dicots, herbaceous dicots, and softwoods) were subjected to various regimes of AFEX™ (ammonia fiber expansion) pre-treatment [AFEX is a trademark of MBI, Lansing (http://www.mbi.org].
25911738	4	89	dep	subgroups	676:684	arg1	grasses					700:706	monocot grasses	692:706	monocot grasses	692:706	Eight distinct biomass types belonging to four different subgroups (i.e. monocot grasses, woody dicots, herbaceous dicots, and softwoods) were subjected to various regimes of AFEX™ (ammonia fiber expansion) pre-treatment [AFEX is a trademark of MBI, Lansing (http://www.mbi.org].
25911738	4	89	dep	subgroups	676:684	arg1	dicots					715:720	woody dicots	709:720	woody dicots	709:720	Eight distinct biomass types belonging to four different subgroups (i.e. monocot grasses, woody dicots, herbaceous dicots, and softwoods) were subjected to various regimes of AFEX™ (ammonia fiber expansion) pre-treatment [AFEX is a trademark of MBI, Lansing (http://www.mbi.org].
25911738	4	89	dep	subgroups	676:684	arg1	dicots					734:739	herbaceous dicots	723:739	herbaceous dicots	723:739	Eight distinct biomass types belonging to four different subgroups (i.e. monocot grasses, woody dicots, herbaceous dicots, and softwoods) were subjected to various regimes of AFEX™ (ammonia fiber expansion) pre-treatment [AFEX is a trademark of MBI, Lansing (http://www.mbi.org].
25911738	5	90	theme	detailed	921:928	arg1	analysis					930:937	detailed analysis	921:937	detailed analysis of close to 200 cell wall glycan epitopes	921:979	This approach allowed detailed analysis of close to 200 cell wall glycan epitopes and their relative extractability using a high-throughput platform.
25911738	7	91	theme	pectin	1385:1390	arg1	epitopes					1407:1414	pectin and xyloglucan epitopes	1385:1414	pectin and xyloglucan epitopes	1385:1414	For most biomass types analysed, such loosening was also evident for other major non-cellulosic components including subclasses of pectin and xyloglucan epitopes.
25911738	6	92	from	loosening	1140:1148	arg1	materials					1235:1243	most plant biomass materials	1216:1243	most plant biomass materials	1216:1243	In general, irrespective of the phylogenetic origin, AFEX™ pre-treatment appeared to cause loosening and improved accessibility of various xylan epitope subclasses in most plant biomass materials studied.
25911738	3	93	theme	glycome	449:455	arg1	profiling					457:465	glycome profiling	449:465	glycome profiling	449:465	Here glycome profiling was employed to determine the relative abundance of matrix polysaccharides in several phylogenetically distinct native and pre-treated plant biomasses.
25911738	9	94	dep	wall	1732:1735	arg1	compositions					1744:1755	glycan compositions	1737:1755	glycan compositions	1737:1755	It was found that monitoring changes in cell wall glycan compositions and their relative extractability for untreated and pre-treated plant biomass can provide an improved understanding of variations in structure and composition of plant cell walls and delineate the role(s) of matrix polysaccharides in cell wall recalcitrance.
25911738	9	95	from	understanding	1859:1871	arg1	composition					1904:1914	composition	1904:1914	composition	1904:1914	It was found that monitoring changes in cell wall glycan compositions and their relative extractability for untreated and pre-treated plant biomass can provide an improved understanding of variations in structure and composition of plant cell walls and delineate the role(s) of matrix polysaccharides in cell wall recalcitrance.
25911738	9	95	from	understanding	1859:1871	arg1	structure					1890:1898	structure	1890:1898	structure	1890:1898	It was found that monitoring changes in cell wall glycan compositions and their relative extractability for untreated and pre-treated plant biomass can provide an improved understanding of variations in structure and composition of plant cell walls and delineate the role(s) of matrix polysaccharides in cell wall recalcitrance.
25911738	4	96	theme	ammonia	801:807	arg1	expansion					815:823	ammonia fiber expansion	801:823	ammonia fiber expansion	801:823	Eight distinct biomass types belonging to four different subgroups (i.e. monocot grasses, woody dicots, herbaceous dicots, and softwoods) were subjected to various regimes of AFEX™ (ammonia fiber expansion) pre-treatment [AFEX is a trademark of MBI, Lansing (http://www.mbi.org].
25911738	5	97	theme	relative	991:998	arg1	extractability					1000:1013	their relative extractability	985:1013	their relative extractability using a high-throughput platform	985:1046	This approach allowed detailed analysis of close to 200 cell wall glycan epitopes and their relative extractability using a high-throughput platform.
25911738	9	98	theme	plant	1919:1923	arg1	walls					1930:1934	plant cell walls	1919:1934	plant cell walls	1919:1934	It was found that monitoring changes in cell wall glycan compositions and their relative extractability for untreated and pre-treated plant biomass can provide an improved understanding of variations in structure and composition of plant cell walls and delineate the role(s) of matrix polysaccharides in cell wall recalcitrance.
25911738	5	99	theme	cell	955:958	arg1	wall					960:963	200 cell wall	951:963	200 cell wall	951:963	This approach allowed detailed analysis of close to 200 cell wall glycan epitopes and their relative extractability using a high-throughput platform.
25911738	7	100	theme	most	1258:1261	arg1	types					1271:1275	most biomass types	1258:1275	most biomass types analysed	1258:1284	For most biomass types analysed, such loosening was also evident for other major non-cellulosic components including subclasses of pectin and xyloglucan epitopes.
25911738	9	101	theme	walls	1930:1934	arg1	composition					1904:1914	composition	1904:1914	composition	1904:1914	It was found that monitoring changes in cell wall glycan compositions and their relative extractability for untreated and pre-treated plant biomass can provide an improved understanding of variations in structure and composition of plant cell walls and delineate the role(s) of matrix polysaccharides in cell wall recalcitrance.
25911738	9	101	theme	walls	1930:1934	arg1	structure					1890:1898	structure	1890:1898	structure	1890:1898	It was found that monitoring changes in cell wall glycan compositions and their relative extractability for untreated and pre-treated plant biomass can provide an improved understanding of variations in structure and composition of plant cell walls and delineate the role(s) of matrix polysaccharides in cell wall recalcitrance.
25911738	8	102	theme	conventional	1646:1657	arg1	analyses					1677:1684	conventional gross composition analyses	1646:1684	conventional gross composition analyses	1646:1684	The studies also demonstrate that AFEX™ pre-treatment significantly reduced cell wall recalcitrance among diverse phylogenies (except softwoods) by inducing structural modifications to polysaccharides that were not detectable by conventional gross composition analyses.
25911738	5	103	theme	glycan	965:970	arg1	epitopes					972:979	close to 200 cell wall glycan epitopes	942:979	close to 200 cell wall glycan epitopes	942:979	This approach allowed detailed analysis of close to 200 cell wall glycan epitopes and their relative extractability using a high-throughput platform.
25911738	6	104	theme	phylogenetic	1081:1092	arg1	origin					1094:1099	the phylogenetic origin	1077:1099	the phylogenetic origin	1077:1099	In general, irrespective of the phylogenetic origin, AFEX™ pre-treatment appeared to cause loosening and improved accessibility of various xylan epitope subclasses in most plant biomass materials studied.
25911738	8	105	theme	composition	1665:1675	arg1	analyses					1677:1684	conventional gross composition analyses	1646:1684	conventional gross composition analyses	1646:1684	The studies also demonstrate that AFEX™ pre-treatment significantly reduced cell wall recalcitrance among diverse phylogenies (except softwoods) by inducing structural modifications to polysaccharides that were not detectable by conventional gross composition analyses.
25911738	4	106	theme	AFEX™	794:798	arg1	[AFEX					840:844	AFEX™ (ammonia fiber expansion) pre-treatment [AFEX	794:844	AFEX™ (ammonia fiber expansion) pre-treatment [AFEX	794:844	Eight distinct biomass types belonging to four different subgroups (i.e. monocot grasses, woody dicots, herbaceous dicots, and softwoods) were subjected to various regimes of AFEX™ (ammonia fiber expansion) pre-treatment [AFEX is a trademark of MBI, Lansing (http://www.mbi.org].
25911738	4	106	theme	AFEX™	794:798	arg1	trademark					851:859	a trademark	849:859	a trademark of MBI, Lansing (http://www.mbi.org]	849:896	Eight distinct biomass types belonging to four different subgroups (i.e. monocot grasses, woody dicots, herbaceous dicots, and softwoods) were subjected to various regimes of AFEX™ (ammonia fiber expansion) pre-treatment [AFEX is a trademark of MBI, Lansing (http://www.mbi.org].
25911738	2	107	theme	pre-treated	324:334	arg1	samples					350:356	both native and pre-treated plant biomass samples	308:356	both native and pre-treated plant biomass samples	308:356	Studies on plant cell walls can be conducted on both native and pre-treated plant biomass samples, allowing an enhanced understanding of these structural and compositional variations.
26538484	4	0	dep	basal	804:808	arg1	feeds					810:814	feeds	810:814	feeds	810:814	The wheat samples were milled through a 5 mm screen and four basal feeds containing 670 g/kg of each selected wheat sample were mixed.
26538484	3	1	theme	wheat	626:630	arg1	samples					632:638	Four European wheat samples	612:638	Four European wheat samples	612:638	Four European wheat samples, with different chemical composition and endosperm characteristics, were used in a broiler experiment.
26538484	2	2	theme	digestive	520:528	arg1	development					536:546	digestive tract development	520:546	digestive tract development of broilers fed four different wheat samples in complete diets	520:609	The aim of this experiment was to examine the effect of steam pelleting on the performance, dietary N-corrected apparent metabolisable energy (AMEn), total tract dry matter retention (DMR), nitrogen retention (NR) and fat digestibility (FD) coefficients, and digestive tract development of broilers fed four different wheat samples in complete diets.
26538484	1	3	theme	different	131:139	arg1	cultivars					147:155	different wheat cultivars	131:155	different wheat cultivars	131:155	Experimental comparisons of the nutritional value of different wheat cultivars commonly use feeds in meal form even though the large-scale broiler producers use steam pelleted feeds.
26538484	2	4	theme	dietary	353:359	arg1	AMEn					404:407	AMEn	404:407	AMEn	404:407	The aim of this experiment was to examine the effect of steam pelleting on the performance, dietary N-corrected apparent metabolisable energy (AMEn), total tract dry matter retention (DMR), nitrogen retention (NR) and fat digestibility (FD) coefficients, and digestive tract development of broilers fed four different wheat samples in complete diets.
26538484	2	4	theme	dietary	353:359	arg1	energy					396:401	dietary N-corrected apparent metabolisable energy	353:401	dietary N-corrected apparent metabolisable energy (AMEn)	353:408	The aim of this experiment was to examine the effect of steam pelleting on the performance, dietary N-corrected apparent metabolisable energy (AMEn), total tract dry matter retention (DMR), nitrogen retention (NR) and fat digestibility (FD) coefficients, and digestive tract development of broilers fed four different wheat samples in complete diets.
26538484	4	5	theme	5	783:783	arg1	mm					785:786	mm	785:786	mm	785:786	The wheat samples were milled through a 5 mm screen and four basal feeds containing 670 g/kg of each selected wheat sample were mixed.
26538484	2	6	theme	wheat	579:583	arg1	samples					585:591	four different wheat samples	564:591	four different wheat samples in complete diets	564:609	The aim of this experiment was to examine the effect of steam pelleting on the performance, dietary N-corrected apparent metabolisable energy (AMEn), total tract dry matter retention (DMR), nitrogen retention (NR) and fat digestibility (FD) coefficients, and digestive tract development of broilers fed four different wheat samples in complete diets.
26538484	1	7	theme	wheat	141:145	arg1	cultivars					147:155	different wheat cultivars	131:155	different wheat cultivars	131:155	Experimental comparisons of the nutritional value of different wheat cultivars commonly use feeds in meal form even though the large-scale broiler producers use steam pelleted feeds.
26538484	6	8	dep	24	1085:1086	arg1	to					1082:1083	to	1082:1083	to	1082:1083	Each diet was fed ad libitum to eight pens of two male Ross 308 broilers from 10 to 24 days of age.
26538484	4	9	theme	mm	785:786	arg1	screen					788:793	a 5 mm screen	781:793	a 5 mm screen	781:793	The wheat samples were milled through a 5 mm screen and four basal feeds containing 670 g/kg of each selected wheat sample were mixed.
26538484	4	10	theme	wheat	853:857	arg1	sample					859:864	each selected wheat sample	839:864	each selected wheat sample	839:864	The wheat samples were milled through a 5 mm screen and four basal feeds containing 670 g/kg of each selected wheat sample were mixed.
26538484	8	11	theme	tract	1250:1254	arg1	development					1214:1224	the development	1210:1224	the development of the gastrointestinal tract of the birds	1210:1267	Feeding different wheat types and pelleting did not (P>0.05) change the development of the gastrointestinal tract of the birds.
26538484	10	12	theme	quality	1528:1534	arg1	characteristics					1536:1550	wheat chemical and quality characteristics	1509:1550	characteristics	1536:1550	Research on the interaction between pelleting and wheat chemical and quality characteristics is warranted.
26538484	2	13	theme	different	569:577	arg1	samples					585:591	four different wheat samples	564:591	four different wheat samples in complete diets	564:609	The aim of this experiment was to examine the effect of steam pelleting on the performance, dietary N-corrected apparent metabolisable energy (AMEn), total tract dry matter retention (DMR), nitrogen retention (NR) and fat digestibility (FD) coefficients, and digestive tract development of broilers fed four different wheat samples in complete diets.
26538484	6	14	theme	male	1051:1054	arg1	broilers					1065:1072	two male Ross 308 broilers	1047:1072	two male Ross 308 broilers from 10 to 24 days of age	1047:1098	Each diet was fed ad libitum to eight pens of two male Ross 308 broilers from 10 to 24 days of age.
26538484	6	15	theme	ad	1019:1020	arg1	libitum					1022:1028	ad libitum	1019:1028	ad libitum	1019:1028	Each diet was fed ad libitum to eight pens of two male Ross 308 broilers from 10 to 24 days of age.
26538484	4	16	theme	selected	844:851	arg1	sample					859:864	each selected wheat sample	839:864	each selected wheat sample	839:864	The wheat samples were milled through a 5 mm screen and four basal feeds containing 670 g/kg of each selected wheat sample were mixed.
26538484	7	17	dep	diets	1118:1122	arg1	improved					1124:1131	improved	1124:1131	diets improved (P0.05)	1118:1139	Feeding pelleted diets improved (P0.05).
26538484	2	18	theme	steam	317:321	arg1	development					536:546	digestive tract development	520:546	digestive tract development of broilers fed four different wheat samples in complete diets	520:609	The aim of this experiment was to examine the effect of steam pelleting on the performance, dietary N-corrected apparent metabolisable energy (AMEn), total tract dry matter retention (DMR), nitrogen retention (NR) and fat digestibility (FD) coefficients, and digestive tract development of broilers fed four different wheat samples in complete diets.
26538484	2	18	theme	steam	317:321	arg1	effect					307:312	the effect	303:312	the effect of steam pelleting on the performance, dietary N-corrected apparent metabolisable energy (AMEn), total tract dry matter retention (DMR), nitrogen retention (NR) and fat digestibility (FD) coefficients	303:513	The aim of this experiment was to examine the effect of steam pelleting on the performance, dietary N-corrected apparent metabolisable energy (AMEn), total tract dry matter retention (DMR), nitrogen retention (NR) and fat digestibility (FD) coefficients, and digestive tract development of broilers fed four different wheat samples in complete diets.
26538484	6	19	theme	Each	1001:1004	arg1	diet					1006:1009	Each diet	1001:1009	Each diet	1001:1009	Each diet was fed ad libitum to eight pens of two male Ross 308 broilers from 10 to 24 days of age.
26538484	2	20	theme	nitrogen	451:458	arg1	NR					471:472	NR	471:472	NR	471:472	The aim of this experiment was to examine the effect of steam pelleting on the performance, dietary N-corrected apparent metabolisable energy (AMEn), total tract dry matter retention (DMR), nitrogen retention (NR) and fat digestibility (FD) coefficients, and digestive tract development of broilers fed four different wheat samples in complete diets.
26538484	2	20	theme	nitrogen	451:458	arg1	retention					460:468	nitrogen retention	451:468	nitrogen retention (NR)	451:473	The aim of this experiment was to examine the effect of steam pelleting on the performance, dietary N-corrected apparent metabolisable energy (AMEn), total tract dry matter retention (DMR), nitrogen retention (NR) and fat digestibility (FD) coefficients, and digestive tract development of broilers fed four different wheat samples in complete diets.
26538484	3	21	theme	different	646:654	arg1	composition					665:675	different chemical composition	646:675	different chemical composition	646:675	Four European wheat samples, with different chemical composition and endosperm characteristics, were used in a broiler experiment.
26538484	2	22	theme	broilers	551:558	arg1	development					536:546	digestive tract development	520:546	digestive tract development of broilers fed four different wheat samples in complete diets	520:609	The aim of this experiment was to examine the effect of steam pelleting on the performance, dietary N-corrected apparent metabolisable energy (AMEn), total tract dry matter retention (DMR), nitrogen retention (NR) and fat digestibility (FD) coefficients, and digestive tract development of broilers fed four different wheat samples in complete diets.
26538484	2	22	theme	broilers	551:558	arg1	effect					307:312	the effect	303:312	the effect of steam pelleting on the performance, dietary N-corrected apparent metabolisable energy (AMEn), total tract dry matter retention (DMR), nitrogen retention (NR) and fat digestibility (FD) coefficients	303:513	The aim of this experiment was to examine the effect of steam pelleting on the performance, dietary N-corrected apparent metabolisable energy (AMEn), total tract dry matter retention (DMR), nitrogen retention (NR) and fat digestibility (FD) coefficients, and digestive tract development of broilers fed four different wheat samples in complete diets.
26538484	2	23	theme	metabolisable	382:394	arg1	AMEn					404:407	AMEn	404:407	AMEn	404:407	The aim of this experiment was to examine the effect of steam pelleting on the performance, dietary N-corrected apparent metabolisable energy (AMEn), total tract dry matter retention (DMR), nitrogen retention (NR) and fat digestibility (FD) coefficients, and digestive tract development of broilers fed four different wheat samples in complete diets.
26538484	2	23	theme	metabolisable	382:394	arg1	energy					396:401	dietary N-corrected apparent metabolisable energy	353:401	dietary N-corrected apparent metabolisable energy (AMEn)	353:408	The aim of this experiment was to examine the effect of steam pelleting on the performance, dietary N-corrected apparent metabolisable energy (AMEn), total tract dry matter retention (DMR), nitrogen retention (NR) and fat digestibility (FD) coefficients, and digestive tract development of broilers fed four different wheat samples in complete diets.
26538484	7	24	dep	improved	1124:1131	arg1	P0.05					1134:1138	P0.05	1134:1138	P0.05	1134:1138	Feeding pelleted diets improved (P0.05).
26538484	4	25	contain	containing	816:825	arg2	g/kg					831:834	670 g/kg	827:834	670 g/kg of each selected wheat sample	827:864	The wheat samples were milled through a 5 mm screen and four basal feeds containing 670 g/kg of each selected wheat sample were mixed.
26538484	4	25	contain	containing	816:825	arg1	basal					804:808	basal	804:808	basal	804:808	The wheat samples were milled through a 5 mm screen and four basal feeds containing 670 g/kg of each selected wheat sample were mixed.
26538484	1	26	theme	cultivars	147:155	arg1	value					122:126	the nutritional value	106:126	the nutritional value of different wheat cultivars	106:155	Experimental comparisons of the nutritional value of different wheat cultivars commonly use feeds in meal form even though the large-scale broiler producers use steam pelleted feeds.
26538484	4	27	theme	sample	859:864	arg1	g/kg					831:834	670 g/kg	827:834	670 g/kg of each selected wheat sample	827:864	The wheat samples were milled through a 5 mm screen and four basal feeds containing 670 g/kg of each selected wheat sample were mixed.
26538484	8	28	theme	wheat	1160:1164	arg1	types					1166:1170	Feeding different wheat types	1142:1170	Feeding different wheat types	1142:1170	Feeding different wheat types and pelleting did not (P>0.05) change the development of the gastrointestinal tract of the birds.
26538484	8	29	dep	change	1203:1208	arg1	P>0.05					1195:1200	P>0.05	1195:1200	P>0.05	1195:1200	Feeding different wheat types and pelleting did not (P>0.05) change the development of the gastrointestinal tract of the birds.
26538484	6	30	theme	age	1096:1098	arg1	days					1088:1091	10 to 24 days	1079:1091	10 to 24 days of age	1079:1098	Each diet was fed ad libitum to eight pens of two male Ross 308 broilers from 10 to 24 days of age.
26538484	2	31	theme	complete	596:603	arg1	diets					605:609	complete diets	596:609	complete diets	596:609	The aim of this experiment was to examine the effect of steam pelleting on the performance, dietary N-corrected apparent metabolisable energy (AMEn), total tract dry matter retention (DMR), nitrogen retention (NR) and fat digestibility (FD) coefficients, and digestive tract development of broilers fed four different wheat samples in complete diets.
26538484	5	32	theme	experimental	981:992	arg1	diets					994:998	eight experimental diets	975:998	eight experimental diets	975:998	The basal feeds were then split into two batches and one of them was steam pelleted resulting in eight experimental diets.
26538484	2	33	theme	tract	530:534	arg1	development					536:546	digestive tract development	520:546	digestive tract development of broilers fed four different wheat samples in complete diets	520:609	The aim of this experiment was to examine the effect of steam pelleting on the performance, dietary N-corrected apparent metabolisable energy (AMEn), total tract dry matter retention (DMR), nitrogen retention (NR) and fat digestibility (FD) coefficients, and digestive tract development of broilers fed four different wheat samples in complete diets.
26538484	2	34	theme	experiment	277:286	arg1	aim					265:267	The aim	261:267	The aim of this experiment	261:286	The aim of this experiment was to examine the effect of steam pelleting on the performance, dietary N-corrected apparent metabolisable energy (AMEn), total tract dry matter retention (DMR), nitrogen retention (NR) and fat digestibility (FD) coefficients, and digestive tract development of broilers fed four different wheat samples in complete diets.
26538484	9	35	theme	pelleted	1364:1371	arg1	feed					1382:1385	pelleted complete feed	1364:1385	pelleted complete feed	1364:1385	The study showed that there were differences between four wheat samples when they were fed in pelleted complete feed, but no differences were observed when fed in mash form complete diets.
26538484	3	36	theme	endosperm	681:689	arg1	characteristics					691:705	endosperm characteristics	681:705	endosperm characteristics	681:705	Four European wheat samples, with different chemical composition and endosperm characteristics, were used in a broiler experiment.
26538484	2	37	theme	fat	479:481	arg1	FD					498:499	FD	498:499	FD	498:499	The aim of this experiment was to examine the effect of steam pelleting on the performance, dietary N-corrected apparent metabolisable energy (AMEn), total tract dry matter retention (DMR), nitrogen retention (NR) and fat digestibility (FD) coefficients, and digestive tract development of broilers fed four different wheat samples in complete diets.
26538484	2	37	theme	fat	479:481	arg1	digestibility					483:495	fat digestibility	479:495	fat digestibility (FD)	479:500	The aim of this experiment was to examine the effect of steam pelleting on the performance, dietary N-corrected apparent metabolisable energy (AMEn), total tract dry matter retention (DMR), nitrogen retention (NR) and fat digestibility (FD) coefficients, and digestive tract development of broilers fed four different wheat samples in complete diets.
26538484	3	38	theme	chemical	656:663	arg1	composition					665:675	different chemical composition	646:675	different chemical composition	646:675	Four European wheat samples, with different chemical composition and endosperm characteristics, were used in a broiler experiment.
26538484	9	39	theme	wheat	1328:1332	arg1	samples					1334:1340	four wheat samples	1323:1340	four wheat samples	1323:1340	The study showed that there were differences between four wheat samples when they were fed in pelleted complete feed, but no differences were observed when fed in mash form complete diets.
26538484	1	40	theme	Experimental	78:89	arg1	comparisons					91:101	Experimental comparisons	78:101	Experimental comparisons of the nutritional value of different wheat cultivars	78:155	Experimental comparisons of the nutritional value of different wheat cultivars commonly use feeds in meal form even though the large-scale broiler producers use steam pelleted feeds.
26538484	1	41	theme	meal	179:182	arg1	form					184:187	meal form	179:187	meal form	179:187	Experimental comparisons of the nutritional value of different wheat cultivars commonly use feeds in meal form even though the large-scale broiler producers use steam pelleted feeds.
26538484	2	42	theme	matter	427:432	arg1	DMR					445:447	DMR	445:447	DMR	445:447	The aim of this experiment was to examine the effect of steam pelleting on the performance, dietary N-corrected apparent metabolisable energy (AMEn), total tract dry matter retention (DMR), nitrogen retention (NR) and fat digestibility (FD) coefficients, and digestive tract development of broilers fed four different wheat samples in complete diets.
26538484	2	42	theme	matter	427:432	arg1	retention					434:442	total tract dry matter retention	411:442	total tract dry matter retention (DMR)	411:448	The aim of this experiment was to examine the effect of steam pelleting on the performance, dietary N-corrected apparent metabolisable energy (AMEn), total tract dry matter retention (DMR), nitrogen retention (NR) and fat digestibility (FD) coefficients, and digestive tract development of broilers fed four different wheat samples in complete diets.
26538484	6	43	from	pens	1039:1042	arg1	days					1088:1091	10 to 24 days	1079:1091	10 to 24 days of age	1079:1098	Each diet was fed ad libitum to eight pens of two male Ross 308 broilers from 10 to 24 days of age.
26538484	1	44	dep	use	166:168	arg1	feeds					170:174	feeds	170:174	use feeds in meal form even though the large-scale broiler producers use steam pelleted feeds	166:258	Experimental comparisons of the nutritional value of different wheat cultivars commonly use feeds in meal form even though the large-scale broiler producers use steam pelleted feeds.
26538484	2	45	theme	apparent	373:380	arg1	AMEn					404:407	AMEn	404:407	AMEn	404:407	The aim of this experiment was to examine the effect of steam pelleting on the performance, dietary N-corrected apparent metabolisable energy (AMEn), total tract dry matter retention (DMR), nitrogen retention (NR) and fat digestibility (FD) coefficients, and digestive tract development of broilers fed four different wheat samples in complete diets.
26538484	2	45	theme	apparent	373:380	arg1	energy					396:401	dietary N-corrected apparent metabolisable energy	353:401	dietary N-corrected apparent metabolisable energy (AMEn)	353:408	The aim of this experiment was to examine the effect of steam pelleting on the performance, dietary N-corrected apparent metabolisable energy (AMEn), total tract dry matter retention (DMR), nitrogen retention (NR) and fat digestibility (FD) coefficients, and digestive tract development of broilers fed four different wheat samples in complete diets.
26538484	0	46	theme	pelleting	19:27	arg1	effect					9:14	the effect	5:14	the effect of pelleting	5:27	Does the effect of pelleting depend on the wheat sample when fed to chickens?
26538484	2	47	theme	dry	423:425	arg1	DMR					445:447	DMR	445:447	DMR	445:447	The aim of this experiment was to examine the effect of steam pelleting on the performance, dietary N-corrected apparent metabolisable energy (AMEn), total tract dry matter retention (DMR), nitrogen retention (NR) and fat digestibility (FD) coefficients, and digestive tract development of broilers fed four different wheat samples in complete diets.
26538484	2	47	theme	dry	423:425	arg1	retention					434:442	total tract dry matter retention	411:442	total tract dry matter retention (DMR)	411:448	The aim of this experiment was to examine the effect of steam pelleting on the performance, dietary N-corrected apparent metabolisable energy (AMEn), total tract dry matter retention (DMR), nitrogen retention (NR) and fat digestibility (FD) coefficients, and digestive tract development of broilers fed four different wheat samples in complete diets.
26538484	2	48	theme	N-corrected	361:371	arg1	AMEn					404:407	AMEn	404:407	AMEn	404:407	The aim of this experiment was to examine the effect of steam pelleting on the performance, dietary N-corrected apparent metabolisable energy (AMEn), total tract dry matter retention (DMR), nitrogen retention (NR) and fat digestibility (FD) coefficients, and digestive tract development of broilers fed four different wheat samples in complete diets.
26538484	2	48	theme	N-corrected	361:371	arg1	energy					396:401	dietary N-corrected apparent metabolisable energy	353:401	dietary N-corrected apparent metabolisable energy (AMEn)	353:408	The aim of this experiment was to examine the effect of steam pelleting on the performance, dietary N-corrected apparent metabolisable energy (AMEn), total tract dry matter retention (DMR), nitrogen retention (NR) and fat digestibility (FD) coefficients, and digestive tract development of broilers fed four different wheat samples in complete diets.
26538484	2	49	theme	tract	417:421	arg1	DMR					445:447	DMR	445:447	DMR	445:447	The aim of this experiment was to examine the effect of steam pelleting on the performance, dietary N-corrected apparent metabolisable energy (AMEn), total tract dry matter retention (DMR), nitrogen retention (NR) and fat digestibility (FD) coefficients, and digestive tract development of broilers fed four different wheat samples in complete diets.
26538484	2	49	theme	tract	417:421	arg1	retention					434:442	total tract dry matter retention	411:442	total tract dry matter retention (DMR)	411:448	The aim of this experiment was to examine the effect of steam pelleting on the performance, dietary N-corrected apparent metabolisable energy (AMEn), total tract dry matter retention (DMR), nitrogen retention (NR) and fat digestibility (FD) coefficients, and digestive tract development of broilers fed four different wheat samples in complete diets.
26538484	8	50	theme	gastrointestinal	1233:1248	arg1	tract					1250:1254	the gastrointestinal tract	1229:1254	the gastrointestinal tract of the birds	1229:1267	Feeding different wheat types and pelleting did not (P>0.05) change the development of the gastrointestinal tract of the birds.
26538484	8	51	theme	different	1150:1158	arg1	types					1166:1170	Feeding different wheat types	1142:1170	Feeding different wheat types	1142:1170	Feeding different wheat types and pelleting did not (P>0.05) change the development of the gastrointestinal tract of the birds.
26538484	2	52	theme	total	411:415	arg1	DMR					445:447	DMR	445:447	DMR	445:447	The aim of this experiment was to examine the effect of steam pelleting on the performance, dietary N-corrected apparent metabolisable energy (AMEn), total tract dry matter retention (DMR), nitrogen retention (NR) and fat digestibility (FD) coefficients, and digestive tract development of broilers fed four different wheat samples in complete diets.
26538484	2	52	theme	total	411:415	arg1	retention					434:442	total tract dry matter retention	411:442	total tract dry matter retention (DMR)	411:448	The aim of this experiment was to examine the effect of steam pelleting on the performance, dietary N-corrected apparent metabolisable energy (AMEn), total tract dry matter retention (DMR), nitrogen retention (NR) and fat digestibility (FD) coefficients, and digestive tract development of broilers fed four different wheat samples in complete diets.
26538484	4	53	theme	wheat	747:751	arg1	samples					753:759	The wheat samples	743:759	The wheat samples	743:759	The wheat samples were milled through a 5 mm screen and four basal feeds containing 670 g/kg of each selected wheat sample were mixed.
26538484	5	54	dep	basal	882:886	arg1	feeds					888:892	feeds	888:892	feeds	888:892	The basal feeds were then split into two batches and one of them was steam pelleted resulting in eight experimental diets.
26538484	3	55	with	samples	632:638	arg1	composition					665:675	different chemical composition	646:675	different chemical composition	646:675	Four European wheat samples, with different chemical composition and endosperm characteristics, were used in a broiler experiment.
26538484	3	55	with	samples	632:638	arg1	characteristics					691:705	endosperm characteristics	681:705	endosperm characteristics	681:705	Four European wheat samples, with different chemical composition and endosperm characteristics, were used in a broiler experiment.
26538484	6	56	theme	Ross	1056:1059	arg1	broilers					1065:1072	two male Ross 308 broilers	1047:1072	two male Ross 308 broilers from 10 to 24 days of age	1047:1098	Each diet was fed ad libitum to eight pens of two male Ross 308 broilers from 10 to 24 days of age.
26538484	2	57	dep	performance	340:350	arg1	coefficients					502:513	coefficients	502:513	coefficients	502:513	The aim of this experiment was to examine the effect of steam pelleting on the performance, dietary N-corrected apparent metabolisable energy (AMEn), total tract dry matter retention (DMR), nitrogen retention (NR) and fat digestibility (FD) coefficients, and digestive tract development of broilers fed four different wheat samples in complete diets.
26538484	9	58	theme	complete	1443:1450	arg1	diets					1452:1456	mash form complete diets	1433:1456	mash form complete diets	1433:1456	The study showed that there were differences between four wheat samples when they were fed in pelleted complete feed, but no differences were observed when fed in mash form complete diets.
26538484	8	59	theme	Feeding	1142:1148	arg1	types					1166:1170	Feeding different wheat types	1142:1170	Feeding different wheat types	1142:1170	Feeding different wheat types and pelleting did not (P>0.05) change the development of the gastrointestinal tract of the birds.
26538484	3	60	theme	broiler	723:729	arg1	experiment					731:740	a broiler experiment	721:740	a broiler experiment	721:740	Four European wheat samples, with different chemical composition and endosperm characteristics, were used in a broiler experiment.
26538484	10	61	from	Research	1459:1466	arg1	interaction					1475:1485	the interaction	1471:1485	the interaction between pelleting and wheat chemical and quality characteristics	1471:1550	Research on the interaction between pelleting and wheat chemical and quality characteristics is warranted.
26538484	3	62	used	used	713:716	arg2	samples					632:638	Four European wheat samples	612:638	Four European wheat samples	612:638	Four European wheat samples, with different chemical composition and endosperm characteristics, were used in a broiler experiment.
26538484	6	63	theme	broilers	1065:1072	arg1	pens					1039:1042	eight pens	1033:1042	eight pens of two male Ross 308 broilers from 10 to 24 days of age	1033:1098	Each diet was fed ad libitum to eight pens of two male Ross 308 broilers from 10 to 24 days of age.
26538484	1	64	theme	nutritional	110:120	arg1	value					122:126	the nutritional value	106:126	the nutritional value of different wheat cultivars	106:155	Experimental comparisons of the nutritional value of different wheat cultivars commonly use feeds in meal form even though the large-scale broiler producers use steam pelleted feeds.
26538484	6	65	from	days	1088:1091	arg1	broilers					1065:1072	two male Ross 308 broilers	1047:1072	two male Ross 308 broilers from 10 to 24 days of age	1047:1098	Each diet was fed ad libitum to eight pens of two male Ross 308 broilers from 10 to 24 days of age.
26538484	6	65	from	days	1088:1091	arg1	pens					1039:1042	eight pens	1033:1042	eight pens of two male Ross 308 broilers from 10 to 24 days of age	1033:1098	Each diet was fed ad libitum to eight pens of two male Ross 308 broilers from 10 to 24 days of age.
26538484	5	66	theme	them	938:941	arg1	them					938:941	them	938:941	them	938:941	The basal feeds were then split into two batches and one of them was steam pelleted resulting in eight experimental diets.
26538484	5	66	theme	them	938:941	arg1	one					931:933	one	931:933	one	931:933	The basal feeds were then split into two batches and one of them was steam pelleted resulting in eight experimental diets.
26538484	1	67	dep	use	235:237	arg1	feeds					254:258	feeds	254:258	use steam pelleted feeds	235:258	Experimental comparisons of the nutritional value of different wheat cultivars commonly use feeds in meal form even though the large-scale broiler producers use steam pelleted feeds.
26538484	8	68	theme	birds	1263:1267	arg1	tract					1250:1254	the gastrointestinal tract	1229:1254	the gastrointestinal tract of the birds	1229:1267	Feeding different wheat types and pelleting did not (P>0.05) change the development of the gastrointestinal tract of the birds.
26538484	1	69	theme	value	122:126	arg1	comparisons					91:101	Experimental comparisons	78:101	Experimental comparisons of the nutritional value of different wheat cultivars	78:155	Experimental comparisons of the nutritional value of different wheat cultivars commonly use feeds in meal form even though the large-scale broiler producers use steam pelleted feeds.
26538484	9	70	dep	fed	1426:1428	arg1	in					1430:1431	in	1430:1431	in	1430:1431	The study showed that there were differences between four wheat samples when they were fed in pelleted complete feed, but no differences were observed when fed in mash form complete diets.
26538484	1	71	theme	large-scale	205:215	arg1	producers					225:233	the large-scale broiler producers	201:233	the large-scale broiler producers	201:233	Experimental comparisons of the nutritional value of different wheat cultivars commonly use feeds in meal form even though the large-scale broiler producers use steam pelleted feeds.
26538484	0	72	theme	wheat	43:47	arg1	sample					49:54	the wheat sample	39:54	the wheat sample	39:54	Does the effect of pelleting depend on the wheat sample when fed to chickens?
26538484	3	73	theme	European	617:624	arg1	samples					632:638	Four European wheat samples	612:638	Four European wheat samples	612:638	Four European wheat samples, with different chemical composition and endosperm characteristics, were used in a broiler experiment.
26538484	5	74	dep	pelleted	953:960	arg1	steam					947:951	steam	947:951	steam	947:951	The basal feeds were then split into two batches and one of them was steam pelleted resulting in eight experimental diets.
26538484	9	75	theme	complete	1373:1380	arg1	feed					1382:1385	pelleted complete feed	1364:1385	pelleted complete feed	1364:1385	The study showed that there were differences between four wheat samples when they were fed in pelleted complete feed, but no differences were observed when fed in mash form complete diets.
26538484	2	76	from	samples	585:591	arg1	diets					605:609	complete diets	596:609	complete diets	596:609	The aim of this experiment was to examine the effect of steam pelleting on the performance, dietary N-corrected apparent metabolisable energy (AMEn), total tract dry matter retention (DMR), nitrogen retention (NR) and fat digestibility (FD) coefficients, and digestive tract development of broilers fed four different wheat samples in complete diets.
26538484	1	77	theme	broiler	217:223	arg1	producers					225:233	the large-scale broiler producers	201:233	the large-scale broiler producers	201:233	Experimental comparisons of the nutritional value of different wheat cultivars commonly use feeds in meal form even though the large-scale broiler producers use steam pelleted feeds.
27214583	0	0	from	Quantification	16:29	arg1	Roots					84:88	Arabidopsis Roots	72:88	Arabidopsis Roots	72:88	High Resolution Quantification of Crystalline Cellulose Accumulation in Arabidopsis Roots to Monitor Tissue-specific Cell Wall Modifications.
27214583	8	1	theme	crystalline	1067:1077	arg1	accumulation					1089:1100	crystalline cellulose accumulation	1067:1100	crystalline cellulose accumulation in cross sections of Arabidopsis thaliana roots	1067:1148	Here, we report a sensitive polarized light-based system for specific quantification of relative light retardance, representing crystalline cellulose accumulation in cross sections of Arabidopsis thaliana roots.
27214583	8	2	theme	light-based	977:987	arg1	system					989:994	a sensitive polarized light-based system	955:994	a sensitive polarized light-based system for specific quantification of relative light retardance	955:1051	Here, we report a sensitive polarized light-based system for specific quantification of relative light retardance, representing crystalline cellulose accumulation in cross sections of Arabidopsis thaliana roots.
27214583	10	3	theme	cellular	1419:1426	arg1	behavior					1428:1435	cellular behavior	1419:1435	cellular behavior	1419:1435	This approach opens a new analytical dimension, shedding light on the link between cell wall composition, cellular behavior and whole-organ growth.
27214583	8	4	theme	specific	1000:1007	arg1	quantification					1009:1022	specific quantification	1000:1022	specific quantification of relative light retardance	1000:1051	Here, we report a sensitive polarized light-based system for specific quantification of relative light retardance, representing crystalline cellulose accumulation in cross sections of Arabidopsis thaliana roots.
27214583	1	5	theme	cell	174:177	arg1	wall					179:182	a cell wall	172:182	a cell wall	172:182	Plant cells are surrounded by a cell wall, the composition of which determines their final size and shape.
27214583	0	6	theme	Cell	117:120	arg1	Modifications					127:139	Tissue-specific Cell Wall Modifications	101:139	Tissue-specific Cell Wall Modifications	101:139	High Resolution Quantification of Crystalline Cellulose Accumulation in Arabidopsis Roots to Monitor Tissue-specific Cell Wall Modifications.
27214583	4	7	used	used	576:579	arg2	methods					564:570	Several methods	556:570	Several methods	556:570	Several methods are used to determine the levels of crystalline cellulose, each bringing both advantages and limitations.
27214583	9	8	theme	direct	1236:1241	arg1	comparisons					1243:1253	direct comparisons	1236:1253	direct comparisons between the different tissues composing the growing root	1236:1310	In this method, the cellular resolution and anatomical data are maintained, enabling direct comparisons between the different tissues composing the growing root.
27214583	10	9	theme	wall	1401:1404	arg1	composition					1406:1416	cell wall composition	1396:1416	cell wall composition	1396:1416	This approach opens a new analytical dimension, shedding light on the link between cell wall composition, cellular behavior and whole-organ growth.
27214583	4	10	theme	cellulose	620:628	arg1	each					631:634	each	631:634	each	631:634	Several methods are used to determine the levels of crystalline cellulose, each bringing both advantages and limitations.
27214583	4	10	theme	cellulose	620:628	arg1	levels					598:603	the levels	594:603	the levels of crystalline cellulose	594:628	Several methods are used to determine the levels of crystalline cellulose, each bringing both advantages and limitations.
27214583	0	11	theme	Tissue-specific	101:115	arg1	Modifications					127:139	Tissue-specific Cell Wall Modifications	101:139	Tissue-specific Cell Wall Modifications	101:139	High Resolution Quantification of Crystalline Cellulose Accumulation in Arabidopsis Roots to Monitor Tissue-specific Cell Wall Modifications.
27214583	8	12	dep	Arabidopsis	1123:1133	arg1	thaliana					1135:1142	thaliana	1135:1142	thaliana	1135:1142	Here, we report a sensitive polarized light-based system for specific quantification of relative light retardance, representing crystalline cellulose accumulation in cross sections of Arabidopsis thaliana roots.
27214583	8	13	theme	cellulose	1079:1087	arg1	accumulation					1089:1100	crystalline cellulose accumulation	1067:1100	crystalline cellulose accumulation in cross sections of Arabidopsis thaliana roots	1067:1148	Here, we report a sensitive polarized light-based system for specific quantification of relative light retardance, representing crystalline cellulose accumulation in cross sections of Arabidopsis thaliana roots.
27214583	6	14	theme	spatiotemporal	837:850	arg1	information					852:862	spatiotemporal information	837:862	spatiotemporal information	837:862	However, they are limited to whole-organ analyses that are deficient in spatiotemporal information.
27214583	4	15	theme	crystalline	608:618	arg1	cellulose					620:628	crystalline cellulose	608:628	crystalline cellulose	608:628	Several methods are used to determine the levels of crystalline cellulose, each bringing both advantages and limitations.
27214583	8	16	theme	cross	1105:1109	arg1	sections					1111:1118	cross sections	1105:1118	cross sections of Arabidopsis thaliana roots	1105:1148	Here, we report a sensitive polarized light-based system for specific quantification of relative light retardance, representing crystalline cellulose accumulation in cross sections of Arabidopsis thaliana roots.
27214583	10	17	theme	analytical	1339:1348	arg1	dimension					1350:1358	a new analytical dimension	1333:1358	a new analytical dimension	1333:1358	This approach opens a new analytical dimension, shedding light on the link between cell wall composition, cellular behavior and whole-organ growth.
27214583	1	18	theme	wall	179:182	arg1	composition					189:199	the composition	185:199	the composition	185:199	Plant cells are surrounded by a cell wall, the composition of which determines their final size and shape.
27214583	0	19	theme	Wall	122:125	arg1	Modifications					127:139	Tissue-specific Cell Wall Modifications	101:139	Tissue-specific Cell Wall Modifications	101:139	High Resolution Quantification of Crystalline Cellulose Accumulation in Arabidopsis Roots to Monitor Tissue-specific Cell Wall Modifications.
27214583	8	20	theme	light	1036:1040	arg1	retardance					1042:1051	relative light retardance	1027:1051	relative light retardance	1027:1051	Here, we report a sensitive polarized light-based system for specific quantification of relative light retardance, representing crystalline cellulose accumulation in cross sections of Arabidopsis thaliana roots.
27214583	10	21	theme	new	1335:1337	arg1	dimension					1350:1358	a new analytical dimension	1333:1358	a new analytical dimension	1333:1358	This approach opens a new analytical dimension, shedding light on the link between cell wall composition, cellular behavior and whole-organ growth.
27214583	2	22	theme	cellulose	400:408	arg1	chains					410:415	cellulose chains	400:415	cellulose chains of amorphous organization	400:441	The cell wall is composed of a complex matrix containing polysaccharides that include cellulose microfibrils that form both crystalline structures and cellulose chains of amorphous organization.
27214583	0	23	theme	Resolution	5:14	arg1	Quantification					16:29	High Resolution Quantification	0:29	High Resolution Quantification of Crystalline Cellulose Accumulation in Arabidopsis Roots	0:88	High Resolution Quantification of Crystalline Cellulose Accumulation in Arabidopsis Roots to Monitor Tissue-specific Cell Wall Modifications.
27214583	8	24	theme	relative	1027:1034	arg1	retardance					1042:1051	relative light retardance	1027:1051	relative light retardance	1027:1051	Here, we report a sensitive polarized light-based system for specific quantification of relative light retardance, representing crystalline cellulose accumulation in cross sections of Arabidopsis thaliana roots.
27214583	0	25	theme	High	0:3	arg1	Quantification					16:29	High Resolution Quantification	0:29	High Resolution Quantification of Crystalline Cellulose Accumulation in Arabidopsis Roots	0:88	High Resolution Quantification of Crystalline Cellulose Accumulation in Arabidopsis Roots to Monitor Tissue-specific Cell Wall Modifications.
27214583	8	26	from	accumulation	1089:1100	arg1	sections					1111:1118	cross sections	1105:1118	cross sections of Arabidopsis thaliana roots	1105:1148	Here, we report a sensitive polarized light-based system for specific quantification of relative light retardance, representing crystalline cellulose accumulation in cross sections of Arabidopsis thaliana roots.
27214583	9	27	theme	different	1267:1275	arg1	tissues					1277:1283	the different tissues	1263:1283	the different tissues composing the growing root	1263:1310	In this method, the cellular resolution and anatomical data are maintained, enabling direct comparisons between the different tissues composing the growing root.
27214583	6	28	from	information	852:862	arg1	deficient					824:832	deficient	824:832	deficient	824:832	However, they are limited to whole-organ analyses that are deficient in spatiotemporal information.
27214583	10	29	theme	cell	1396:1399	arg1	composition					1406:1416	cell wall composition	1396:1416	cell wall composition	1396:1416	This approach opens a new analytical dimension, shedding light on the link between cell wall composition, cellular behavior and whole-organ growth.
27214583	9	30	theme	anatomical	1195:1204	arg1	data					1206:1209	anatomical data	1195:1209	anatomical data	1195:1209	In this method, the cellular resolution and anatomical data are maintained, enabling direct comparisons between the different tissues composing the growing root.
27214583	8	31	theme	retardance	1042:1051	arg1	quantification					1009:1022	specific quantification	1000:1022	specific quantification of relative light retardance	1000:1051	Here, we report a sensitive polarized light-based system for specific quantification of relative light retardance, representing crystalline cellulose accumulation in cross sections of Arabidopsis thaliana roots.
27214583	7	32	theme	live	883:886	arg1	imaging					888:894	live imaging	883:894	live imaging	883:894	Others relying on live imaging, are limited by the use of imprecise dyes.
27214583	8	33	theme	Arabidopsis	1123:1133	arg1	roots					1144:1148	Arabidopsis thaliana roots	1123:1148	Arabidopsis thaliana roots	1123:1148	Here, we report a sensitive polarized light-based system for specific quantification of relative light retardance, representing crystalline cellulose accumulation in cross sections of Arabidopsis thaliana roots.
27214583	2	34	theme	complex	280:286	arg1	matrix					288:293	a complex matrix	278:293	a complex matrix containing polysaccharides that include cellulose microfibrils that form both crystalline structures and cellulose chains of amorphous organization	278:441	The cell wall is composed of a complex matrix containing polysaccharides that include cellulose microfibrils that form both crystalline structures and cellulose chains of amorphous organization.
27214583	2	35	theme	organization	430:441	arg1	structures					385:394	crystalline structures	373:394	crystalline structures	373:394	The cell wall is composed of a complex matrix containing polysaccharides that include cellulose microfibrils that form both crystalline structures and cellulose chains of amorphous organization.
27214583	2	35	theme	organization	430:441	arg1	chains					410:415	cellulose chains	400:415	cellulose chains of amorphous organization	400:441	The cell wall is composed of a complex matrix containing polysaccharides that include cellulose microfibrils that form both crystalline structures and cellulose chains of amorphous organization.
27214583	0	36	theme	Cellulose	46:54	arg1	Accumulation					56:67	Crystalline Cellulose Accumulation	34:67	Crystalline Cellulose Accumulation	34:67	High Resolution Quantification of Crystalline Cellulose Accumulation in Arabidopsis Roots to Monitor Tissue-specific Cell Wall Modifications.
27214583	2	37	theme	cellulose	335:343	arg1	microfibrils					345:356	cellulose microfibrils	335:356	cellulose microfibrils that form both crystalline structures and cellulose chains of amorphous organization	335:441	The cell wall is composed of a complex matrix containing polysaccharides that include cellulose microfibrils that form both crystalline structures and cellulose chains of amorphous organization.
27214583	5	38	theme	total	748:752	arg1	cellulose					754:762	the total cellulose	744:762	the total cellulose	744:762	Some can distinguish the proportion of crystalline regions within the total cellulose.
27214583	3	39	theme	cellulose	467:475	arg1	fibers					477:482	the cellulose fibers	463:482	the cellulose fibers	463:482	The orientation of the cellulose fibers and their concentrations dictate the mechanical properties of the cell.
27214583	9	40	from	method	1159:1164	arg1	resolution					1180:1189	the cellular resolution	1167:1189	the cellular resolution	1167:1189	In this method, the cellular resolution and anatomical data are maintained, enabling direct comparisons between the different tissues composing the growing root.
27214583	9	40	from	method	1159:1164	arg1	maintained					1215:1224	maintained	1215:1224	maintained	1215:1224	In this method, the cellular resolution and anatomical data are maintained, enabling direct comparisons between the different tissues composing the growing root.
27214583	2	41	theme	amorphous	420:428	arg1	organization					430:441	amorphous organization	420:441	amorphous organization	420:441	The cell wall is composed of a complex matrix containing polysaccharides that include cellulose microfibrils that form both crystalline structures and cellulose chains of amorphous organization.
27214583	0	42	theme	Crystalline	34:44	arg1	Accumulation					56:67	Crystalline Cellulose Accumulation	34:67	Crystalline Cellulose Accumulation	34:67	High Resolution Quantification of Crystalline Cellulose Accumulation in Arabidopsis Roots to Monitor Tissue-specific Cell Wall Modifications.
27214583	4	43	theme	Several	556:562	arg1	methods					564:570	Several methods	556:570	Several methods	556:570	Several methods are used to determine the levels of crystalline cellulose, each bringing both advantages and limitations.
27214583	8	44	theme	roots	1144:1148	arg1	sections					1111:1118	cross sections	1105:1118	cross sections of Arabidopsis thaliana roots	1105:1148	Here, we report a sensitive polarized light-based system for specific quantification of relative light retardance, representing crystalline cellulose accumulation in cross sections of Arabidopsis thaliana roots.
27214583	9	45	theme	growing	1299:1305	arg1	root					1307:1310	the growing root	1295:1310	the growing root	1295:1310	In this method, the cellular resolution and anatomical data are maintained, enabling direct comparisons between the different tissues composing the growing root.
27214583	6	46	theme	whole-organ	794:804	arg1	analyses					806:813	whole-organ analyses	794:813	whole-organ analyses that are deficient in spatiotemporal information	794:862	However, they are limited to whole-organ analyses that are deficient in spatiotemporal information.
27214583	3	47	theme	fibers	477:482	arg1	concentrations					494:507	their concentrations	488:507	their concentrations	488:507	The orientation of the cellulose fibers and their concentrations dictate the mechanical properties of the cell.
27214583	3	47	theme	fibers	477:482	arg1	orientation					448:458	The orientation	444:458	The orientation of the cellulose fibers	444:482	The orientation of the cellulose fibers and their concentrations dictate the mechanical properties of the cell.
27214583	9	48	theme	cellular	1171:1178	arg1	resolution					1180:1189	the cellular resolution	1167:1189	the cellular resolution	1167:1189	In this method, the cellular resolution and anatomical data are maintained, enabling direct comparisons between the different tissues composing the growing root.
27214583	9	48	theme	cellular	1171:1178	arg1	maintained					1215:1224	maintained	1215:1224	maintained	1215:1224	In this method, the cellular resolution and anatomical data are maintained, enabling direct comparisons between the different tissues composing the growing root.
27214583	0	49	theme	Accumulation	56:67	arg1	Quantification					16:29	High Resolution Quantification	0:29	High Resolution Quantification of Crystalline Cellulose Accumulation in Arabidopsis Roots	0:88	High Resolution Quantification of Crystalline Cellulose Accumulation in Arabidopsis Roots to Monitor Tissue-specific Cell Wall Modifications.
27214583	2	50	contain	containing	295:304	arg2	polysaccharides					306:320	polysaccharides	306:320	polysaccharides that include cellulose microfibrils that form both crystalline structures and cellulose chains of amorphous organization	306:441	The cell wall is composed of a complex matrix containing polysaccharides that include cellulose microfibrils that form both crystalline structures and cellulose chains of amorphous organization.
27214583	2	50	contain	containing	295:304	arg1	matrix					288:293	a complex matrix	278:293	a complex matrix containing polysaccharides that include cellulose microfibrils that form both crystalline structures and cellulose chains of amorphous organization	278:441	The cell wall is composed of a complex matrix containing polysaccharides that include cellulose microfibrils that form both crystalline structures and cellulose chains of amorphous organization.
27214583	9	51	from	maintained	1215:1224	arg1	method					1159:1164	this method	1154:1164	this method	1154:1164	In this method, the cellular resolution and anatomical data are maintained, enabling direct comparisons between the different tissues composing the growing root.
27214583	1	52	theme	Plant	142:146	arg1	cells					148:152	Plant cells	142:152	Plant cells	142:152	Plant cells are surrounded by a cell wall, the composition of which determines their final size and shape.
27214583	5	53	theme	crystalline	717:727	arg1	regions					729:735	crystalline regions	717:735	crystalline regions	717:735	Some can distinguish the proportion of crystalline regions within the total cellulose.
27214583	10	54	theme	whole-organ	1441:1451	arg1	growth					1453:1458	whole-organ growth	1441:1458	whole-organ growth	1441:1458	This approach opens a new analytical dimension, shedding light on the link between cell wall composition, cellular behavior and whole-organ growth.
27214583	1	55	theme	final	227:231	arg1	size					233:236	their final size	221:236	their final size	221:236	Plant cells are surrounded by a cell wall, the composition of which determines their final size and shape.
27214583	5	56	theme	regions	729:735	arg1	proportion					703:712	the proportion	699:712	the proportion of crystalline regions within the total cellulose	699:762	Some can distinguish the proportion of crystalline regions within the total cellulose.
27214583	2	57	theme	cell	253:256	arg1	wall					258:261	The cell wall	249:261	The cell wall	249:261	The cell wall is composed of a complex matrix containing polysaccharides that include cellulose microfibrils that form both crystalline structures and cellulose chains of amorphous organization.
27214583	8	58	theme	polarized	967:975	arg1	system					989:994	a sensitive polarized light-based system	955:994	a sensitive polarized light-based system for specific quantification of relative light retardance	955:1051	Here, we report a sensitive polarized light-based system for specific quantification of relative light retardance, representing crystalline cellulose accumulation in cross sections of Arabidopsis thaliana roots.
27214583	7	59	theme	imprecise	923:931	arg1	dyes					933:936	imprecise dyes	923:936	imprecise dyes	923:936	Others relying on live imaging, are limited by the use of imprecise dyes.
27214583	8	60	theme	sensitive	957:965	arg1	system					989:994	a sensitive polarized light-based system	955:994	a sensitive polarized light-based system for specific quantification of relative light retardance	955:1051	Here, we report a sensitive polarized light-based system for specific quantification of relative light retardance, representing crystalline cellulose accumulation in cross sections of Arabidopsis thaliana roots.
27214583	2	61	theme	crystalline	373:383	arg1	structures					385:394	crystalline structures	373:394	crystalline structures	373:394	The cell wall is composed of a complex matrix containing polysaccharides that include cellulose microfibrils that form both crystalline structures and cellulose chains of amorphous organization.
27214583	7	62	theme	dyes	933:936	arg1	use					916:918	the use	912:918	the use of imprecise dyes	912:936	Others relying on live imaging, are limited by the use of imprecise dyes.
27214583	0	63	theme	Arabidopsis	72:82	arg1	Roots					84:88	Arabidopsis Roots	72:88	Arabidopsis Roots	72:88	High Resolution Quantification of Crystalline Cellulose Accumulation in Arabidopsis Roots to Monitor Tissue-specific Cell Wall Modifications.
27214583	6	64	from	deficient	824:832	arg1	information					852:862	spatiotemporal information	837:862	spatiotemporal information	837:862	However, they are limited to whole-organ analyses that are deficient in spatiotemporal information.
27214583	3	65	theme	cell	550:553	arg1	properties					532:541	the mechanical properties	517:541	the mechanical properties of the cell	517:553	The orientation of the cellulose fibers and their concentrations dictate the mechanical properties of the cell.
27214583	3	66	theme	mechanical	521:530	arg1	properties					532:541	the mechanical properties	517:541	the mechanical properties of the cell	517:553	The orientation of the cellulose fibers and their concentrations dictate the mechanical properties of the cell.
28396923	7	0	dep	Escherichia	1001:1011	arg1	coli					1013:1016	coli	1013:1016	coli	1013:1016	To determine if EPS production could alter bacterial Hg tolerance, Escherichia coli K-12 strains and their EPS defective mutants were tested by the disc inhibition assay.
28396923	6	1	theme	uptake	856:861	arg1	capacities					863:872	Hg uptake capacities	853:872	Hg uptake capacities	853:872	Hg uptake capacities ranged from 0.005 to 0.454 mmol Hg/g for the different EPS.
28396923	10	2	theme	Hg	1532:1533	arg1	binding					1521:1527	binding	1521:1527	binding of Hg to EPS	1521:1540	Together these data indicate that binding of Hg to EPS affords a low level of resistance to the producing bacteria.
28396923	5	3	theme	saturable	832:840	arg1	mechanism					842:850	a saturable mechanism	830:850	a saturable mechanism	830:850	The data showed that EPS sorbed up to 82% of Hg from solution, that this sorption was dependent on EPS composition, and that sorption was a saturable mechanism.
28396923	5	3	theme	saturable	832:840	arg1	sorption					817:824	sorption	817:824	sorption	817:824	The data showed that EPS sorbed up to 82% of Hg from solution, that this sorption was dependent on EPS composition, and that sorption was a saturable mechanism.
28396923	6	4	theme	Hg	853:854	arg1	capacities					863:872	Hg uptake capacities	853:872	Hg uptake capacities	853:872	Hg uptake capacities ranged from 0.005 to 0.454 mmol Hg/g for the different EPS.
28396923	4	5	theme	Hg	679:680	arg1	sorption					682:689	Hg sorption	679:689	Hg sorption	679:689	Purified EPS with different chemical compositions produced by bacterial isolates from microbial mats in French Polynesian atolls and deep-sea hydrothermal vents were assessed for Hg sorption.
28396923	9	6	theme	Hg-binding	1459:1468	arg1	hexosamine					1445:1454	hexosamine	1445:1454	hexosamine	1445:1454	These experiments identified colanic acid and hexosamine as Hg-binding moieties in EPS.
28396923	9	6	theme	Hg-binding	1459:1468	arg1	moieties					1470:1477	Hg-binding moieties	1459:1477	Hg-binding moieties in EPS	1459:1484	These experiments identified colanic acid and hexosamine as Hg-binding moieties in EPS.
28396923	9	6	theme	Hg-binding	1459:1468	arg1	acid					1436:1439	colanic acid	1428:1439	colanic acid	1428:1439	These experiments identified colanic acid and hexosamine as Hg-binding moieties in EPS.
28396923	4	7	theme	hydrothermal	642:653	arg1	vents					655:659	deep-sea hydrothermal vents	633:659	deep-sea hydrothermal vents	633:659	Purified EPS with different chemical compositions produced by bacterial isolates from microbial mats in French Polynesian atolls and deep-sea hydrothermal vents were assessed for Hg sorption.
28396923	0	8	theme	Hg	76:77	arg1	tolerance					79:87	Hg tolerance	76:87	Hg tolerance	76:87	Binding of Hg by bacterial extracellular polysaccharide: a possible role in Hg tolerance.
28396923	7	9	theme	defective	1045:1053	arg1	mutants					1055:1061	their EPS defective mutants	1035:1061	their EPS defective mutants	1035:1061	To determine if EPS production could alter bacterial Hg tolerance, Escherichia coli K-12 strains and their EPS defective mutants were tested by the disc inhibition assay.
28396923	2	10	theme	production	271:280	arg1	potential					222:230	The potential	218:230	The potential of extracellular polysaccharides (EPS) production by bacteria as a mechanism of Hg tolerance	218:323	The potential of extracellular polysaccharides (EPS) production by bacteria as a mechanism of Hg tolerance has not been previously investigated.
28396923	4	11	theme	bacterial	562:570	arg1	isolates					572:579	bacterial isolates	562:579	bacterial isolates from microbial mats in French Polynesian atolls and deep-sea hydrothermal vents	562:659	Purified EPS with different chemical compositions produced by bacterial isolates from microbial mats in French Polynesian atolls and deep-sea hydrothermal vents were assessed for Hg sorption.
28396923	1	12	theme	tolerance	142:150	arg1	mechanisms					115:124	adaptive mechanisms	106:124	adaptive mechanisms of mercury (Hg) tolerance	106:150	Bacteria employ adaptive mechanisms of mercury (Hg) tolerance to survive in environments containing elevated Hg concentrations.
28396923	6	13	theme	different	919:927	arg1	EPS					929:931	the different EPS	915:931	the different EPS	915:931	Hg uptake capacities ranged from 0.005 to 0.454 mmol Hg/g for the different EPS.
28396923	4	14	dep	different	518:526	arg1	chemical					528:535	chemical	528:535	chemical	528:535	Purified EPS with different chemical compositions produced by bacterial isolates from microbial mats in French Polynesian atolls and deep-sea hydrothermal vents were assessed for Hg sorption.
28396923	5	15	theme	Hg	737:738	arg1	Hg					737:738	Hg	737:738	Hg	737:738	The data showed that EPS sorbed up to 82% of Hg from solution, that this sorption was dependent on EPS composition, and that sorption was a saturable mechanism.
28396923	5	15	theme	Hg	737:738	arg1	%					732:732	up to 82%	724:732	up to 82% of Hg	724:738	The data showed that EPS sorbed up to 82% of Hg from solution, that this sorption was dependent on EPS composition, and that sorption was a saturable mechanism.
28396923	8	16	from	decline	1320:1326	arg1	EPS					1345:1347	EPS	1345:1347	EPS produced by the mutants relative to cell biomass	1345:1396	Mercury inhibited growth in a dose-dependent manner with wild-type strains having smaller (~1 mm), but statistically significant, zones of inhibition than various mutants and this difference was related to a 2-fold decline in the amount of EPS produced by the mutants relative to cell biomass.
28396923	8	16	from	decline	1320:1326	arg1	amount					1335:1340	the amount	1331:1340	the amount of EPS produced by the mutants relative to cell biomass	1331:1396	Mercury inhibited growth in a dose-dependent manner with wild-type strains having smaller (~1 mm), but statistically significant, zones of inhibition than various mutants and this difference was related to a 2-fold decline in the amount of EPS produced by the mutants relative to cell biomass.
28396923	3	17	theme	EPS	423:425	arg1	Hg					432:433	bacterial EPS sorb Hg	413:433	bacterial EPS sorb Hg	413:433	The objectives of this study were to determine if bacterial EPS sorb Hg, and if so does sorption provide protection against Hg toxicity.
28396923	6	18	theme	0.454 mmol	895:904	arg1	Hg/g					906:909	0.454 mmol Hg/g	895:909	0.454 mmol Hg/g for the different EPS	895:931	Hg uptake capacities ranged from 0.005 to 0.454 mmol Hg/g for the different EPS.
28396923	7	19	theme	K-12	1018:1021	arg1	strains					1023:1029	Escherichia coli K-12 strains	1001:1029	Escherichia coli K-12 strains	1001:1029	To determine if EPS production could alter bacterial Hg tolerance, Escherichia coli K-12 strains and their EPS defective mutants were tested by the disc inhibition assay.
28396923	3	20	dep	provide	460:466	arg1	if					440:441	if	440:441	if	440:441	The objectives of this study were to determine if bacterial EPS sorb Hg, and if so does sorption provide protection against Hg toxicity.
28396923	10	21	theme	low	1552:1554	arg1	level					1556:1560	a low level	1550:1560	a low level of resistance to the producing bacteria	1550:1600	Together these data indicate that binding of Hg to EPS affords a low level of resistance to the producing bacteria.
28396923	4	22	theme	different	518:526	arg1	compositions					537:548	different chemical compositions	518:548	different chemical compositions produced by bacterial isolates from microbial mats in French Polynesian atolls and deep-sea hydrothermal vents	518:659	Purified EPS with different chemical compositions produced by bacterial isolates from microbial mats in French Polynesian atolls and deep-sea hydrothermal vents were assessed for Hg sorption.
28396923	8	23	contain	having	1180:1185	arg1	strains					1172:1178	wild-type strains	1162:1178	wild-type strains having smaller (~1 mm), but statistically significant, zones of inhibition than various mutants	1162:1274	Mercury inhibited growth in a dose-dependent manner with wild-type strains having smaller (~1 mm), but statistically significant, zones of inhibition than various mutants and this difference was related to a 2-fold decline in the amount of EPS produced by the mutants relative to cell biomass.
28396923	8	23	contain	having	1180:1185	arg2	zones					1235:1239	smaller (~1 mm), but statistically significant, zones	1187:1239	smaller (~1 mm), but statistically significant, zones of inhibition than various mutants	1187:1274	Mercury inhibited growth in a dose-dependent manner with wild-type strains having smaller (~1 mm), but statistically significant, zones of inhibition than various mutants and this difference was related to a 2-fold decline in the amount of EPS produced by the mutants relative to cell biomass.
28396923	8	24	dep	smaller	1187:1193	arg1	~1 mm					1196:1200	~1 mm	1196:1200	~1 mm	1196:1200	Mercury inhibited growth in a dose-dependent manner with wild-type strains having smaller (~1 mm), but statistically significant, zones of inhibition than various mutants and this difference was related to a 2-fold decline in the amount of EPS produced by the mutants relative to cell biomass.
28396923	0	25	from	role	68:71	arg1	tolerance					79:87	Hg tolerance	76:87	Hg tolerance	76:87	Binding of Hg by bacterial extracellular polysaccharide: a possible role in Hg tolerance.
28396923	5	26	theme	EPS	791:793	arg1	composition					795:805	EPS composition	791:805	EPS composition	791:805	The data showed that EPS sorbed up to 82% of Hg from solution, that this sorption was dependent on EPS composition, and that sorption was a saturable mechanism.
28396923	7	27	theme	Escherichia	1001:1011	arg1	strains					1023:1029	Escherichia coli K-12 strains	1001:1029	Escherichia coli K-12 strains	1001:1029	To determine if EPS production could alter bacterial Hg tolerance, Escherichia coli K-12 strains and their EPS defective mutants were tested by the disc inhibition assay.
28396923	9	28	from	moieties	1470:1477	arg1	EPS					1482:1484	EPS	1482:1484	EPS	1482:1484	These experiments identified colanic acid and hexosamine as Hg-binding moieties in EPS.
28396923	4	29	theme	Polynesian	611:620	arg1	atolls					622:627	French Polynesian atolls	604:627	French Polynesian atolls	604:627	Purified EPS with different chemical compositions produced by bacterial isolates from microbial mats in French Polynesian atolls and deep-sea hydrothermal vents were assessed for Hg sorption.
28396923	0	30	theme	Hg	11:12	arg1	Binding					0:6	Binding	0:6	Binding of Hg by bacterial extracellular polysaccharide: a possible role in Hg tolerance.	0:88	Binding of Hg by bacterial extracellular polysaccharide: a possible role in Hg tolerance.
28396923	7	31	theme	disc	1082:1085	arg1	assay					1098:1102	the disc inhibition assay	1078:1102	the disc inhibition assay	1078:1102	To determine if EPS production could alter bacterial Hg tolerance, Escherichia coli K-12 strains and their EPS defective mutants were tested by the disc inhibition assay.
28396923	8	32	theme	inhibition	1244:1253	arg1	zones					1235:1239	smaller (~1 mm), but statistically significant, zones	1187:1239	smaller (~1 mm), but statistically significant, zones of inhibition than various mutants	1187:1274	Mercury inhibited growth in a dose-dependent manner with wild-type strains having smaller (~1 mm), but statistically significant, zones of inhibition than various mutants and this difference was related to a 2-fold decline in the amount of EPS produced by the mutants relative to cell biomass.
28396923	0	33	theme	extracellular	27:39	arg1	polysaccharide					41:54	bacterial extracellular polysaccharide	17:54	bacterial extracellular polysaccharide	17:54	Binding of Hg by bacterial extracellular polysaccharide: a possible role in Hg tolerance.
28396923	2	34	theme	tolerance	315:323	arg1	mechanism					299:307	a mechanism	297:307	a mechanism of Hg tolerance	297:323	The potential of extracellular polysaccharides (EPS) production by bacteria as a mechanism of Hg tolerance has not been previously investigated.
28396923	1	35	theme	elevated	190:197	arg1	concentrations					202:215	elevated Hg concentrations	190:215	elevated Hg concentrations	190:215	Bacteria employ adaptive mechanisms of mercury (Hg) tolerance to survive in environments containing elevated Hg concentrations.
28396923	8	36	theme	smaller	1187:1193	arg1	zones					1235:1239	smaller (~1 mm), but statistically significant, zones	1187:1239	smaller (~1 mm), but statistically significant, zones of inhibition than various mutants	1187:1274	Mercury inhibited growth in a dose-dependent manner with wild-type strains having smaller (~1 mm), but statistically significant, zones of inhibition than various mutants and this difference was related to a 2-fold decline in the amount of EPS produced by the mutants relative to cell biomass.
28396923	3	37	theme	sorb	427:430	arg1	Hg					432:433	bacterial EPS sorb Hg	413:433	bacterial EPS sorb Hg	413:433	The objectives of this study were to determine if bacterial EPS sorb Hg, and if so does sorption provide protection against Hg toxicity.
28396923	0	38	theme	bacterial	17:25	arg1	polysaccharide					41:54	bacterial extracellular polysaccharide	17:54	bacterial extracellular polysaccharide	17:54	Binding of Hg by bacterial extracellular polysaccharide: a possible role in Hg tolerance.
28396923	5	39	dep	82	730:731	arg1	to					727:728	to	727:728	to	727:728	The data showed that EPS sorbed up to 82% of Hg from solution, that this sorption was dependent on EPS composition, and that sorption was a saturable mechanism.
28396923	8	40	theme	relative	1373:1380	arg1	mutants					1365:1371	the mutants	1361:1371	the mutants relative to cell biomass	1361:1396	Mercury inhibited growth in a dose-dependent manner with wild-type strains having smaller (~1 mm), but statistically significant, zones of inhibition than various mutants and this difference was related to a 2-fold decline in the amount of EPS produced by the mutants relative to cell biomass.
28396923	7	41	theme	inhibition	1087:1096	arg1	assay					1098:1102	the disc inhibition assay	1078:1102	the disc inhibition assay	1078:1102	To determine if EPS production could alter bacterial Hg tolerance, Escherichia coli K-12 strains and their EPS defective mutants were tested by the disc inhibition assay.
28396923	7	42	theme	EPS	950:952	arg1	production					954:963	EPS production	950:963	EPS production	950:963	To determine if EPS production could alter bacterial Hg tolerance, Escherichia coli K-12 strains and their EPS defective mutants were tested by the disc inhibition assay.
28396923	1	43	theme	Hg	199:200	arg1	concentrations					202:215	elevated Hg concentrations	190:215	elevated Hg concentrations	190:215	Bacteria employ adaptive mechanisms of mercury (Hg) tolerance to survive in environments containing elevated Hg concentrations.
28396923	8	44	theme	wild-type	1162:1170	arg1	strains					1172:1178	wild-type strains	1162:1178	wild-type strains having smaller (~1 mm), but statistically significant, zones of inhibition than various mutants	1162:1274	Mercury inhibited growth in a dose-dependent manner with wild-type strains having smaller (~1 mm), but statistically significant, zones of inhibition than various mutants and this difference was related to a 2-fold decline in the amount of EPS produced by the mutants relative to cell biomass.
28396923	7	45	theme	bacterial	977:985	arg1	tolerance					990:998	bacterial Hg tolerance	977:998	bacterial Hg tolerance	977:998	To determine if EPS production could alter bacterial Hg tolerance, Escherichia coli K-12 strains and their EPS defective mutants were tested by the disc inhibition assay.
28396923	3	46	theme	bacterial	413:421	arg1	Hg					432:433	bacterial EPS sorb Hg	413:433	bacterial EPS sorb Hg	413:433	The objectives of this study were to determine if bacterial EPS sorb Hg, and if so does sorption provide protection against Hg toxicity.
28396923	8	47	theme	significant	1222:1232	arg1	zones					1235:1239	smaller (~1 mm), but statistically significant, zones	1187:1239	smaller (~1 mm), but statistically significant, zones of inhibition than various mutants	1187:1274	Mercury inhibited growth in a dose-dependent manner with wild-type strains having smaller (~1 mm), but statistically significant, zones of inhibition than various mutants and this difference was related to a 2-fold decline in the amount of EPS produced by the mutants relative to cell biomass.
28396923	3	48	theme	study	386:390	arg1	objectives					367:376	The objectives	363:376	The objectives of this study	363:390	The objectives of this study were to determine if bacterial EPS sorb Hg, and if so does sorption provide protection against Hg toxicity.
28396923	7	49	theme	Hg	987:988	arg1	tolerance					990:998	bacterial Hg tolerance	977:998	bacterial Hg tolerance	977:998	To determine if EPS production could alter bacterial Hg tolerance, Escherichia coli K-12 strains and their EPS defective mutants were tested by the disc inhibition assay.
28396923	8	50	theme	cell	1385:1388	arg1	biomass					1390:1396	cell biomass	1385:1396	cell biomass	1385:1396	Mercury inhibited growth in a dose-dependent manner with wild-type strains having smaller (~1 mm), but statistically significant, zones of inhibition than various mutants and this difference was related to a 2-fold decline in the amount of EPS produced by the mutants relative to cell biomass.
28396923	8	51	theme	EPS	1345:1347	arg1	EPS					1345:1347	EPS	1345:1347	EPS produced by the mutants relative to cell biomass	1345:1396	Mercury inhibited growth in a dose-dependent manner with wild-type strains having smaller (~1 mm), but statistically significant, zones of inhibition than various mutants and this difference was related to a 2-fold decline in the amount of EPS produced by the mutants relative to cell biomass.
28396923	8	51	theme	EPS	1345:1347	arg1	amount					1335:1340	the amount	1331:1340	the amount of EPS produced by the mutants relative to cell biomass	1331:1396	Mercury inhibited growth in a dose-dependent manner with wild-type strains having smaller (~1 mm), but statistically significant, zones of inhibition than various mutants and this difference was related to a 2-fold decline in the amount of EPS produced by the mutants relative to cell biomass.
28396923	1	52	theme	adaptive	106:113	arg1	mechanisms					115:124	adaptive mechanisms	106:124	adaptive mechanisms of mercury (Hg) tolerance	106:150	Bacteria employ adaptive mechanisms of mercury (Hg) tolerance to survive in environments containing elevated Hg concentrations.
28396923	4	53	theme	Purified	500:507	arg1	EPS					509:511	Purified EPS	500:511	Purified EPS with different chemical compositions produced by bacterial isolates from microbial mats in French Polynesian atolls and deep-sea hydrothermal vents	500:659	Purified EPS with different chemical compositions produced by bacterial isolates from microbial mats in French Polynesian atolls and deep-sea hydrothermal vents were assessed for Hg sorption.
28396923	8	54	theme	2-fold	1313:1318	arg1	decline					1320:1326	a 2-fold decline	1311:1326	a 2-fold decline in the amount of EPS produced by the mutants relative to cell biomass	1311:1396	Mercury inhibited growth in a dose-dependent manner with wild-type strains having smaller (~1 mm), but statistically significant, zones of inhibition than various mutants and this difference was related to a 2-fold decline in the amount of EPS produced by the mutants relative to cell biomass.
28396923	2	55	theme	Hg	312:313	arg1	tolerance					315:323	Hg tolerance	312:323	Hg tolerance	312:323	The potential of extracellular polysaccharides (EPS) production by bacteria as a mechanism of Hg tolerance has not been previously investigated.
28396923	4	56	theme	microbial	586:594	arg1	mats					596:599	microbial mats	586:599	microbial mats in French Polynesian atolls and deep-sea hydrothermal vents	586:659	Purified EPS with different chemical compositions produced by bacterial isolates from microbial mats in French Polynesian atolls and deep-sea hydrothermal vents were assessed for Hg sorption.
28396923	1	57	contain	containing	179:188	arg2	concentrations					202:215	elevated Hg concentrations	190:215	elevated Hg concentrations	190:215	Bacteria employ adaptive mechanisms of mercury (Hg) tolerance to survive in environments containing elevated Hg concentrations.
28396923	1	57	contain	containing	179:188	arg1	environments					166:177	environments	166:177	environments containing elevated Hg concentrations	166:215	Bacteria employ adaptive mechanisms of mercury (Hg) tolerance to survive in environments containing elevated Hg concentrations.
28396923	2	58	theme	polysaccharides	249:263	arg1	production					271:280	extracellular polysaccharides (EPS) production	235:280	extracellular polysaccharides (EPS) production by bacteria	235:292	The potential of extracellular polysaccharides (EPS) production by bacteria as a mechanism of Hg tolerance has not been previously investigated.
28396923	10	59	theme	resistance	1565:1574	arg1	level					1556:1560	a low level	1550:1560	a low level of resistance to the producing bacteria	1550:1600	Together these data indicate that binding of Hg to EPS affords a low level of resistance to the producing bacteria.
28396923	9	60	theme	colanic	1428:1434	arg1	hexosamine					1445:1454	hexosamine	1445:1454	hexosamine	1445:1454	These experiments identified colanic acid and hexosamine as Hg-binding moieties in EPS.
28396923	9	60	theme	colanic	1428:1434	arg1	moieties					1470:1477	Hg-binding moieties	1459:1477	Hg-binding moieties in EPS	1459:1484	These experiments identified colanic acid and hexosamine as Hg-binding moieties in EPS.
28396923	9	60	theme	colanic	1428:1434	arg1	acid					1436:1439	colanic acid	1428:1439	colanic acid	1428:1439	These experiments identified colanic acid and hexosamine as Hg-binding moieties in EPS.
28396923	8	61	theme	various	1260:1266	arg1	mutants					1268:1274	various mutants	1260:1274	various mutants	1260:1274	Mercury inhibited growth in a dose-dependent manner with wild-type strains having smaller (~1 mm), but statistically significant, zones of inhibition than various mutants and this difference was related to a 2-fold decline in the amount of EPS produced by the mutants relative to cell biomass.
28396923	8	62	theme	dose-dependent	1135:1148	arg1	manner					1150:1155	a dose-dependent manner	1133:1155	a dose-dependent manner	1133:1155	Mercury inhibited growth in a dose-dependent manner with wild-type strains having smaller (~1 mm), but statistically significant, zones of inhibition than various mutants and this difference was related to a 2-fold decline in the amount of EPS produced by the mutants relative to cell biomass.
28396923	0	63	theme	possible	59:66	arg1	role					68:71	a possible role	57:71	Binding of Hg by bacterial extracellular polysaccharide: a possible role in Hg tolerance.	0:88	Binding of Hg by bacterial extracellular polysaccharide: a possible role in Hg tolerance.
28396923	4	64	with	EPS	509:511	arg1	compositions					537:548	different chemical compositions	518:548	different chemical compositions produced by bacterial isolates from microbial mats in French Polynesian atolls and deep-sea hydrothermal vents	518:659	Purified EPS with different chemical compositions produced by bacterial isolates from microbial mats in French Polynesian atolls and deep-sea hydrothermal vents were assessed for Hg sorption.
28396923	2	65	theme	extracellular	235:247	arg1	EPS					266:268	EPS	266:268	EPS	266:268	The potential of extracellular polysaccharides (EPS) production by bacteria as a mechanism of Hg tolerance has not been previously investigated.
28396923	2	65	theme	extracellular	235:247	arg1	polysaccharides					249:263	extracellular polysaccharides	235:263	extracellular polysaccharides (EPS) production by bacteria	235:292	The potential of extracellular polysaccharides (EPS) production by bacteria as a mechanism of Hg tolerance has not been previously investigated.
28396923	10	66	theme	producing	1583:1591	arg1	bacteria					1593:1600	the producing bacteria	1579:1600	the producing bacteria	1579:1600	Together these data indicate that binding of Hg to EPS affords a low level of resistance to the producing bacteria.
28396923	1	67	theme	mercury	129:135	arg1	tolerance					142:150	mercury (Hg) tolerance	129:150	mercury (Hg) tolerance	129:150	Bacteria employ adaptive mechanisms of mercury (Hg) tolerance to survive in environments containing elevated Hg concentrations.
28396923	4	68	theme	deep-sea	633:640	arg1	vents					655:659	deep-sea hydrothermal vents	633:659	deep-sea hydrothermal vents	633:659	Purified EPS with different chemical compositions produced by bacterial isolates from microbial mats in French Polynesian atolls and deep-sea hydrothermal vents were assessed for Hg sorption.
28396923	4	69	from	mats	596:599	arg1	atolls					622:627	French Polynesian atolls	604:627	French Polynesian atolls	604:627	Purified EPS with different chemical compositions produced by bacterial isolates from microbial mats in French Polynesian atolls and deep-sea hydrothermal vents were assessed for Hg sorption.
28396923	4	69	from	mats	596:599	arg1	isolates					572:579	bacterial isolates	562:579	bacterial isolates from microbial mats in French Polynesian atolls and deep-sea hydrothermal vents	562:659	Purified EPS with different chemical compositions produced by bacterial isolates from microbial mats in French Polynesian atolls and deep-sea hydrothermal vents were assessed for Hg sorption.
28396923	4	69	from	mats	596:599	arg1	vents					655:659	deep-sea hydrothermal vents	633:659	deep-sea hydrothermal vents	633:659	Purified EPS with different chemical compositions produced by bacterial isolates from microbial mats in French Polynesian atolls and deep-sea hydrothermal vents were assessed for Hg sorption.
28396923	0	70	dep	Binding	0:6	arg1	role					68:71	a possible role	57:71	Binding of Hg by bacterial extracellular polysaccharide: a possible role in Hg tolerance.	0:88	Binding of Hg by bacterial extracellular polysaccharide: a possible role in Hg tolerance.
28396923	3	71	theme	Hg	487:488	arg1	toxicity					490:497	Hg toxicity	487:497	Hg toxicity	487:497	The objectives of this study were to determine if bacterial EPS sorb Hg, and if so does sorption provide protection against Hg toxicity.
29034863	3	0	dep	Serratia	507:514	arg1	marcescens					516:525	marcescens	516:525	marcescens	516:525	Using 1H and 13C NMR spectroscopy, we found that the O-polysaccharide (O-antigen) of this bacterium is comprised of a disaccharide repeat (O-unit) of d-glucose and 2-O-acetyl-l-rhamnose, which is shared by Serratia marcescens O6.
29034863	2	1	from	CJG	178:180	arg1	genus					204:208	this genus	199:208	this genus	199:208	Comamonas aquatica CJG (C. aquatica) in this genus is able to absorb low-density lipoprotein but not high-density lipoprotein of human serum.
29034863	4	2	with	consistent	755:764	arg1	structure					785:793	the O-antigen structure	771:793	the O-antigen structure determined here	771:809	The O-antigen gene cluster of C. aquatica, which is located between coaX and tnp4 genes, contains rhamnose synthesis genes, glycosyl and acetyl transferase genes, and ATP-binding cassette transporter genes, and therefore is consistent with the O-antigen structure determined here.
29034863	3	3	theme	NMR	318:320	arg1	spectroscopy					322:333	NMR spectroscopy	318:333	NMR spectroscopy	318:333	Using 1H and 13C NMR spectroscopy, we found that the O-polysaccharide (O-antigen) of this bacterium is comprised of a disaccharide repeat (O-unit) of d-glucose and 2-O-acetyl-l-rhamnose, which is shared by Serratia marcescens O6.
29034863	4	4	theme	transferase	675:685	arg1	genes					687:691	glycosyl and acetyl transferase genes	655:691	glycosyl and acetyl transferase genes	655:691	The O-antigen gene cluster of C. aquatica, which is located between coaX and tnp4 genes, contains rhamnose synthesis genes, glycosyl and acetyl transferase genes, and ATP-binding cassette transporter genes, and therefore is consistent with the O-antigen structure determined here.
29034863	1	5	theme	Genus	61:65	arg1	Comamonas					67:75	Genus Comamonas	61:75	Genus Comamonas	61:75	Genus Comamonas is a group of bacteria that are able to degrade a variety of environmental waste.
29034863	1	5	theme	Genus	61:65	arg1	group					82:86	a group	80:86	a group of bacteria that are able to degrade a variety of environmental waste	80:156	Genus Comamonas is a group of bacteria that are able to degrade a variety of environmental waste.
29034863	1	5	theme	Genus	61:65	arg1	bacteria					91:98	bacteria	91:98	bacteria that are able to degrade a variety of environmental waste	91:156	Genus Comamonas is a group of bacteria that are able to degrade a variety of environmental waste.
29034863	3	6	theme	disaccharide	419:430	arg1	repeat					432:437	a disaccharide repeat	417:437	a disaccharide repeat (O-unit)	417:446	Using 1H and 13C NMR spectroscopy, we found that the O-polysaccharide (O-antigen) of this bacterium is comprised of a disaccharide repeat (O-unit) of d-glucose and 2-O-acetyl-l-rhamnose, which is shared by Serratia marcescens O6.
29034863	3	6	theme	disaccharide	419:430	arg1	O-unit					440:445	O-unit	440:445	O-unit	440:445	Using 1H and 13C NMR spectroscopy, we found that the O-polysaccharide (O-antigen) of this bacterium is comprised of a disaccharide repeat (O-unit) of d-glucose and 2-O-acetyl-l-rhamnose, which is shared by Serratia marcescens O6.
29034863	2	7	theme	low-density	228:238	arg1	lipoprotein					240:250	low-density lipoprotein	228:250	low-density lipoprotein but not high-density lipoprotein of human serum	228:298	Comamonas aquatica CJG (C. aquatica) in this genus is able to absorb low-density lipoprotein but not high-density lipoprotein of human serum.
29034863	1	8	theme	environmental	138:150	arg1	waste					152:156	environmental waste	138:156	environmental waste	138:156	Genus Comamonas is a group of bacteria that are able to degrade a variety of environmental waste.
29034863	4	9	contain	contains	620:627	arg1	consistent					755:764	consistent	755:764	consistent	755:764	The O-antigen gene cluster of C. aquatica, which is located between coaX and tnp4 genes, contains rhamnose synthesis genes, glycosyl and acetyl transferase genes, and ATP-binding cassette transporter genes, and therefore is consistent with the O-antigen structure determined here.
29034863	4	9	contain	contains	620:627	arg2	genes					648:652	rhamnose synthesis genes	629:652	rhamnose synthesis genes	629:652	The O-antigen gene cluster of C. aquatica, which is located between coaX and tnp4 genes, contains rhamnose synthesis genes, glycosyl and acetyl transferase genes, and ATP-binding cassette transporter genes, and therefore is consistent with the O-antigen structure determined here.
29034863	4	9	contain	contains	620:627	arg2	genes					687:691	glycosyl and acetyl transferase genes	655:691	glycosyl and acetyl transferase genes	655:691	The O-antigen gene cluster of C. aquatica, which is located between coaX and tnp4 genes, contains rhamnose synthesis genes, glycosyl and acetyl transferase genes, and ATP-binding cassette transporter genes, and therefore is consistent with the O-antigen structure determined here.
29034863	4	9	contain	contains	620:627	arg2	genes					731:735	ATP-binding cassette transporter genes	698:735	ATP-binding cassette transporter genes	698:735	The O-antigen gene cluster of C. aquatica, which is located between coaX and tnp4 genes, contains rhamnose synthesis genes, glycosyl and acetyl transferase genes, and ATP-binding cassette transporter genes, and therefore is consistent with the O-antigen structure determined here.
29034863	4	9	contain	contains	620:627	arg1	located					583:589	located	583:589	located	583:589	The O-antigen gene cluster of C. aquatica, which is located between coaX and tnp4 genes, contains rhamnose synthesis genes, glycosyl and acetyl transferase genes, and ATP-binding cassette transporter genes, and therefore is consistent with the O-antigen structure determined here.
29034863	4	9	contain	contains	620:627	arg1	cluster					550:556	The O-antigen gene cluster	531:556	The O-antigen gene cluster	531:556	The O-antigen gene cluster of C. aquatica, which is located between coaX and tnp4 genes, contains rhamnose synthesis genes, glycosyl and acetyl transferase genes, and ATP-binding cassette transporter genes, and therefore is consistent with the O-antigen structure determined here.
29034863	3	10	theme	2-O-acetyl-l-rhamnose	465:485	arg1	repeat					432:437	a disaccharide repeat	417:437	a disaccharide repeat (O-unit)	417:446	Using 1H and 13C NMR spectroscopy, we found that the O-polysaccharide (O-antigen) of this bacterium is comprised of a disaccharide repeat (O-unit) of d-glucose and 2-O-acetyl-l-rhamnose, which is shared by Serratia marcescens O6.
29034863	3	10	theme	2-O-acetyl-l-rhamnose	465:485	arg1	O-unit					440:445	O-unit	440:445	O-unit	440:445	Using 1H and 13C NMR spectroscopy, we found that the O-polysaccharide (O-antigen) of this bacterium is comprised of a disaccharide repeat (O-unit) of d-glucose and 2-O-acetyl-l-rhamnose, which is shared by Serratia marcescens O6.
29034863	1	11	theme	waste	152:156	arg1	variety					127:133	a variety	125:133	a variety of environmental waste	125:156	Genus Comamonas is a group of bacteria that are able to degrade a variety of environmental waste.
29034863	1	11	theme	waste	152:156	arg1	waste					152:156	environmental waste	138:156	environmental waste	138:156	Genus Comamonas is a group of bacteria that are able to degrade a variety of environmental waste.
29034863	0	12	theme	O-antigen	4:12	arg1	structure					14:22	The O-antigen structure	0:22	The O-antigen structure of bacterium Comamonas aquatica	0:54	The O-antigen structure of bacterium Comamonas aquatica CJG.
29034863	4	13	theme	O-antigen	775:783	arg1	structure					785:793	the O-antigen structure	771:793	the O-antigen structure determined here	771:809	The O-antigen gene cluster of C. aquatica, which is located between coaX and tnp4 genes, contains rhamnose synthesis genes, glycosyl and acetyl transferase genes, and ATP-binding cassette transporter genes, and therefore is consistent with the O-antigen structure determined here.
29034863	4	14	theme	glycosyl	655:662	arg1	genes					687:691	glycosyl and acetyl transferase genes	655:691	glycosyl and acetyl transferase genes	655:691	The O-antigen gene cluster of C. aquatica, which is located between coaX and tnp4 genes, contains rhamnose synthesis genes, glycosyl and acetyl transferase genes, and ATP-binding cassette transporter genes, and therefore is consistent with the O-antigen structure determined here.
29034863	4	15	theme	synthesis	638:646	arg1	genes					648:652	rhamnose synthesis genes	629:652	rhamnose synthesis genes	629:652	The O-antigen gene cluster of C. aquatica, which is located between coaX and tnp4 genes, contains rhamnose synthesis genes, glycosyl and acetyl transferase genes, and ATP-binding cassette transporter genes, and therefore is consistent with the O-antigen structure determined here.
29034863	3	16	theme	Serratia	507:514	arg1	O6					527:528	Serratia marcescens O6	507:528	Serratia marcescens O6	507:528	Using 1H and 13C NMR spectroscopy, we found that the O-polysaccharide (O-antigen) of this bacterium is comprised of a disaccharide repeat (O-unit) of d-glucose and 2-O-acetyl-l-rhamnose, which is shared by Serratia marcescens O6.
29034863	2	17	theme	high-density	260:271	arg1	lipoprotein					273:283	high-density lipoprotein	260:283	low-density lipoprotein but not high-density lipoprotein of human serum	228:298	Comamonas aquatica CJG (C. aquatica) in this genus is able to absorb low-density lipoprotein but not high-density lipoprotein of human serum.
29034863	4	18	theme	aquatica	564:571	arg1	located					583:589	located	583:589	located	583:589	The O-antigen gene cluster of C. aquatica, which is located between coaX and tnp4 genes, contains rhamnose synthesis genes, glycosyl and acetyl transferase genes, and ATP-binding cassette transporter genes, and therefore is consistent with the O-antigen structure determined here.
29034863	4	18	theme	aquatica	564:571	arg1	consistent					755:764	consistent	755:764	consistent	755:764	The O-antigen gene cluster of C. aquatica, which is located between coaX and tnp4 genes, contains rhamnose synthesis genes, glycosyl and acetyl transferase genes, and ATP-binding cassette transporter genes, and therefore is consistent with the O-antigen structure determined here.
29034863	4	18	theme	aquatica	564:571	arg1	cluster					550:556	The O-antigen gene cluster	531:556	The O-antigen gene cluster	531:556	The O-antigen gene cluster of C. aquatica, which is located between coaX and tnp4 genes, contains rhamnose synthesis genes, glycosyl and acetyl transferase genes, and ATP-binding cassette transporter genes, and therefore is consistent with the O-antigen structure determined here.
29034863	4	19	theme	gene	545:548	arg1	located					583:589	located	583:589	located	583:589	The O-antigen gene cluster of C. aquatica, which is located between coaX and tnp4 genes, contains rhamnose synthesis genes, glycosyl and acetyl transferase genes, and ATP-binding cassette transporter genes, and therefore is consistent with the O-antigen structure determined here.
29034863	4	19	theme	gene	545:548	arg1	consistent					755:764	consistent	755:764	consistent	755:764	The O-antigen gene cluster of C. aquatica, which is located between coaX and tnp4 genes, contains rhamnose synthesis genes, glycosyl and acetyl transferase genes, and ATP-binding cassette transporter genes, and therefore is consistent with the O-antigen structure determined here.
29034863	4	19	theme	gene	545:548	arg1	cluster					550:556	The O-antigen gene cluster	531:556	The O-antigen gene cluster	531:556	The O-antigen gene cluster of C. aquatica, which is located between coaX and tnp4 genes, contains rhamnose synthesis genes, glycosyl and acetyl transferase genes, and ATP-binding cassette transporter genes, and therefore is consistent with the O-antigen structure determined here.
29034863	2	20	theme	Comamonas	159:167	arg1	CJG					178:180	Comamonas aquatica CJG	159:180	Comamonas aquatica CJG (C. aquatica) in this genus	159:208	Comamonas aquatica CJG (C. aquatica) in this genus is able to absorb low-density lipoprotein but not high-density lipoprotein of human serum.
29034863	1	21	theme	bacteria	91:98	arg1	Comamonas					67:75	Genus Comamonas	61:75	Genus Comamonas	61:75	Genus Comamonas is a group of bacteria that are able to degrade a variety of environmental waste.
29034863	1	21	theme	bacteria	91:98	arg1	group					82:86	a group	80:86	a group of bacteria that are able to degrade a variety of environmental waste	80:156	Genus Comamonas is a group of bacteria that are able to degrade a variety of environmental waste.
29034863	1	21	theme	bacteria	91:98	arg1	bacteria					91:98	bacteria	91:98	bacteria that are able to degrade a variety of environmental waste	91:156	Genus Comamonas is a group of bacteria that are able to degrade a variety of environmental waste.
29034863	0	22	theme	Comamonas	37:45	arg1	aquatica					47:54	bacterium Comamonas aquatica	27:54	bacterium Comamonas aquatica	27:54	The O-antigen structure of bacterium Comamonas aquatica CJG.
29034863	3	23	theme	bacterium	391:399	arg1	O-antigen					372:380	O-antigen	372:380	O-antigen	372:380	Using 1H and 13C NMR spectroscopy, we found that the O-polysaccharide (O-antigen) of this bacterium is comprised of a disaccharide repeat (O-unit) of d-glucose and 2-O-acetyl-l-rhamnose, which is shared by Serratia marcescens O6.
29034863	3	23	theme	bacterium	391:399	arg1	O-polysaccharide					354:369	the O-polysaccharide	350:369	the O-polysaccharide (O-antigen) of this bacterium	350:399	Using 1H and 13C NMR spectroscopy, we found that the O-polysaccharide (O-antigen) of this bacterium is comprised of a disaccharide repeat (O-unit) of d-glucose and 2-O-acetyl-l-rhamnose, which is shared by Serratia marcescens O6.
29034863	0	24	theme	bacterium	27:35	arg1	aquatica					47:54	bacterium Comamonas aquatica	27:54	bacterium Comamonas aquatica	27:54	The O-antigen structure of bacterium Comamonas aquatica CJG.
29034863	3	25	dep	1H	307:308	arg1	spectroscopy					322:333	NMR spectroscopy	318:333	NMR spectroscopy	318:333	Using 1H and 13C NMR spectroscopy, we found that the O-polysaccharide (O-antigen) of this bacterium is comprised of a disaccharide repeat (O-unit) of d-glucose and 2-O-acetyl-l-rhamnose, which is shared by Serratia marcescens O6.
29034863	4	26	theme	rhamnose	629:636	arg1	genes					648:652	rhamnose synthesis genes	629:652	rhamnose synthesis genes	629:652	The O-antigen gene cluster of C. aquatica, which is located between coaX and tnp4 genes, contains rhamnose synthesis genes, glycosyl and acetyl transferase genes, and ATP-binding cassette transporter genes, and therefore is consistent with the O-antigen structure determined here.
29034863	0	27	theme	aquatica	47:54	arg1	structure					14:22	The O-antigen structure	0:22	The O-antigen structure of bacterium Comamonas aquatica	0:54	The O-antigen structure of bacterium Comamonas aquatica CJG.
29034863	4	28	theme	cassette	710:717	arg1	transporter					719:729	ATP-binding cassette transporter	698:729	ATP-binding cassette transporter genes	698:735	The O-antigen gene cluster of C. aquatica, which is located between coaX and tnp4 genes, contains rhamnose synthesis genes, glycosyl and acetyl transferase genes, and ATP-binding cassette transporter genes, and therefore is consistent with the O-antigen structure determined here.
29034863	2	29	theme	serum	294:298	arg1	lipoprotein					273:283	high-density lipoprotein	260:283	low-density lipoprotein but not high-density lipoprotein of human serum	228:298	Comamonas aquatica CJG (C. aquatica) in this genus is able to absorb low-density lipoprotein but not high-density lipoprotein of human serum.
29034863	4	30	theme	acetyl	668:673	arg1	transferase					675:685	acetyl transferase	668:685	acetyl transferase	668:685	The O-antigen gene cluster of C. aquatica, which is located between coaX and tnp4 genes, contains rhamnose synthesis genes, glycosyl and acetyl transferase genes, and ATP-binding cassette transporter genes, and therefore is consistent with the O-antigen structure determined here.
29034863	2	31	dep	CJG	178:180	arg1	aquatica					186:193	C. aquatica	183:193	C. aquatica	183:193	Comamonas aquatica CJG (C. aquatica) in this genus is able to absorb low-density lipoprotein but not high-density lipoprotein of human serum.
29034863	4	32	theme	ATP-binding	698:708	arg1	transporter					719:729	ATP-binding cassette transporter	698:729	ATP-binding cassette transporter genes	698:735	The O-antigen gene cluster of C. aquatica, which is located between coaX and tnp4 genes, contains rhamnose synthesis genes, glycosyl and acetyl transferase genes, and ATP-binding cassette transporter genes, and therefore is consistent with the O-antigen structure determined here.
29034863	2	33	theme	human	288:292	arg1	serum					294:298	human serum	288:298	human serum	288:298	Comamonas aquatica CJG (C. aquatica) in this genus is able to absorb low-density lipoprotein but not high-density lipoprotein of human serum.
29034863	4	34	theme	O-antigen	535:543	arg1	located					583:589	located	583:589	located	583:589	The O-antigen gene cluster of C. aquatica, which is located between coaX and tnp4 genes, contains rhamnose synthesis genes, glycosyl and acetyl transferase genes, and ATP-binding cassette transporter genes, and therefore is consistent with the O-antigen structure determined here.
29034863	4	34	theme	O-antigen	535:543	arg1	consistent					755:764	consistent	755:764	consistent	755:764	The O-antigen gene cluster of C. aquatica, which is located between coaX and tnp4 genes, contains rhamnose synthesis genes, glycosyl and acetyl transferase genes, and ATP-binding cassette transporter genes, and therefore is consistent with the O-antigen structure determined here.
29034863	4	34	theme	O-antigen	535:543	arg1	cluster					550:556	The O-antigen gene cluster	531:556	The O-antigen gene cluster	531:556	The O-antigen gene cluster of C. aquatica, which is located between coaX and tnp4 genes, contains rhamnose synthesis genes, glycosyl and acetyl transferase genes, and ATP-binding cassette transporter genes, and therefore is consistent with the O-antigen structure determined here.
29034863	2	35	theme	aquatica	169:176	arg1	CJG					178:180	Comamonas aquatica CJG	159:180	Comamonas aquatica CJG (C. aquatica) in this genus	159:208	Comamonas aquatica CJG (C. aquatica) in this genus is able to absorb low-density lipoprotein but not high-density lipoprotein of human serum.
29034863	4	36	theme	tnp4	608:611	arg1	genes					613:617	tnp4 genes	608:617	tnp4 genes	608:617	The O-antigen gene cluster of C. aquatica, which is located between coaX and tnp4 genes, contains rhamnose synthesis genes, glycosyl and acetyl transferase genes, and ATP-binding cassette transporter genes, and therefore is consistent with the O-antigen structure determined here.
29034863	4	37	theme	transporter	719:729	arg1	genes					731:735	ATP-binding cassette transporter genes	698:735	ATP-binding cassette transporter genes	698:735	The O-antigen gene cluster of C. aquatica, which is located between coaX and tnp4 genes, contains rhamnose synthesis genes, glycosyl and acetyl transferase genes, and ATP-binding cassette transporter genes, and therefore is consistent with the O-antigen structure determined here.
29034863	3	38	theme	d-glucose	451:459	arg1	repeat					432:437	a disaccharide repeat	417:437	a disaccharide repeat (O-unit)	417:446	Using 1H and 13C NMR spectroscopy, we found that the O-polysaccharide (O-antigen) of this bacterium is comprised of a disaccharide repeat (O-unit) of d-glucose and 2-O-acetyl-l-rhamnose, which is shared by Serratia marcescens O6.
29034863	3	38	theme	d-glucose	451:459	arg1	O-unit					440:445	O-unit	440:445	O-unit	440:445	Using 1H and 13C NMR spectroscopy, we found that the O-polysaccharide (O-antigen) of this bacterium is comprised of a disaccharide repeat (O-unit) of d-glucose and 2-O-acetyl-l-rhamnose, which is shared by Serratia marcescens O6.
26572334	6	0	theme	isoproturon	872:882	arg1	isoproturon					872:882	isoproturon	872:882	isoproturon loaded	872:889	After a series of eight irrigations leached about 10% of isoproturon loaded.
26572334	6	0	theme	isoproturon	872:882	arg1	%					867:867	about 10%	859:867	about 10% of isoproturon loaded	859:889	After a series of eight irrigations leached about 10% of isoproturon loaded.
26572334	3	1	theme	encapsulation	520:532	arg1	efficiency					534:543	75% encapsulation efficiency	516:543	75% encapsulation efficiency	516:543	The hydrophilic CMS/MMT composite systems were used for herbicide, i.e. isoproturon encapsulation (ca. 75% encapsulation efficiency).
26572334	0	2	theme	isoproturon	100:110	arg1	Properties					63:72	Properties	63:72	Properties	63:72	Carboxymethyl starch/montmorillonite composite microparticles: Properties and controlled release of isoproturon.
26572334	0	2	theme	isoproturon	100:110	arg1	release					89:95	controlled release	78:95	controlled release of isoproturon	78:110	Carboxymethyl starch/montmorillonite composite microparticles: Properties and controlled release of isoproturon.
26572334	4	3	theme	ca.	707:709	arg1	24h					711:713	ca. 24h	707:713	ca. 24h	707:713	The herbicide release rate from CMS/MMT composites in water was significantly reduced when compared to commercial isoproturon: 95% released after ca. 700 h and ca. 24h, respectively.
26572334	5	4	from	Leaching	730:737	arg1	soil					742:745	soil	742:745	soil	742:745	Leaching in soil from composite systems was relatively slower than release in water.
26572334	5	5	from	release	797:803	arg1	water					808:812	water	808:812	water	808:812	Leaching in soil from composite systems was relatively slower than release in water.
26572334	2	6	from	starch	359:364	arg1	CMS					335:337	CMS	335:337	CMS	335:337	For preparing nanocomposite granules carboxymethyl starch (CMS) from native potato starch as well as CMS from amylopectin has been used.
26572334	2	6	from	starch	359:364	arg1	starch					327:332	carboxymethyl starch	313:332	carboxymethyl starch (CMS) from native potato starch as well as CMS from amylopectin	313:396	For preparing nanocomposite granules carboxymethyl starch (CMS) from native potato starch as well as CMS from amylopectin has been used.
26572334	2	6	from	starch	359:364	arg1	CMS					377:379	CMS	377:379	carboxymethyl starch (CMS) from native potato starch as well as CMS from amylopectin	313:396	For preparing nanocomposite granules carboxymethyl starch (CMS) from native potato starch as well as CMS from amylopectin has been used.
26572334	3	7	used	used	460:463	arg2	systems					447:453	The hydrophilic CMS/MMT composite systems	413:453	The hydrophilic CMS/MMT composite systems	413:453	The hydrophilic CMS/MMT composite systems were used for herbicide, i.e. isoproturon encapsulation (ca. 75% encapsulation efficiency).
26572334	7	8	theme	herbicide	952:960	arg1	leaching					940:947	the potential leaching	926:947	the potential leaching of herbicide	926:960	The CMS/MMT carriers could reduce the potential leaching of herbicide and beneficially reduce pollution of the environment.
26572334	7	9	theme	environment	1003:1013	arg1	pollution					986:994	pollution	986:994	pollution of the environment	986:1013	The CMS/MMT carriers could reduce the potential leaching of herbicide and beneficially reduce pollution of the environment.
26572334	3	10	theme	75	516:517	arg1	%					518:518	%	518:518	%	518:518	The hydrophilic CMS/MMT composite systems were used for herbicide, i.e. isoproturon encapsulation (ca. 75% encapsulation efficiency).
26572334	5	11	theme	composite	752:760	arg1	systems					762:768	composite systems	752:768	composite systems	752:768	Leaching in soil from composite systems was relatively slower than release in water.
26572334	4	12	from	rate	569:572	arg1	water					601:605	water	601:605	water	601:605	The herbicide release rate from CMS/MMT composites in water was significantly reduced when compared to commercial isoproturon: 95% released after ca. 700 h and ca. 24h, respectively.
26572334	2	13	theme	carboxymethyl	313:325	arg1	CMS					335:337	CMS	335:337	CMS	335:337	For preparing nanocomposite granules carboxymethyl starch (CMS) from native potato starch as well as CMS from amylopectin has been used.
26572334	2	13	theme	carboxymethyl	313:325	arg1	starch					327:332	carboxymethyl starch	313:332	carboxymethyl starch (CMS) from native potato starch as well as CMS from amylopectin	313:396	For preparing nanocomposite granules carboxymethyl starch (CMS) from native potato starch as well as CMS from amylopectin has been used.
26572334	1	14	contain	containing	193:202	arg2	montmorillonite					211:225	sodium montmorillonite	204:225	sodium montmorillonite (MMT)	204:231	Preparation of novel high substituted carboxymethyl starch-based microparticles containing sodium montmorillonite (MMT) by crosslinking with Al(3+) was described.
26572334	1	14	contain	containing	193:202	arg1	microparticles					178:191	novel high substituted carboxymethyl starch-based microparticles	128:191	novel high substituted carboxymethyl starch-based microparticles containing sodium montmorillonite (MMT)	128:231	Preparation of novel high substituted carboxymethyl starch-based microparticles containing sodium montmorillonite (MMT) by crosslinking with Al(3+) was described.
26572334	1	14	contain	containing	193:202	arg2	MMT					228:230	MMT	228:230	MMT	228:230	Preparation of novel high substituted carboxymethyl starch-based microparticles containing sodium montmorillonite (MMT) by crosslinking with Al(3+) was described.
26572334	7	15	theme	potential	930:938	arg1	leaching					940:947	the potential leaching	926:947	the potential leaching of herbicide	926:960	The CMS/MMT carriers could reduce the potential leaching of herbicide and beneficially reduce pollution of the environment.
26572334	2	16	theme	potato	352:357	arg1	starch					359:364	native potato starch	345:364	native potato starch	345:364	For preparing nanocomposite granules carboxymethyl starch (CMS) from native potato starch as well as CMS from amylopectin has been used.
26572334	4	17	from	composites	587:596	arg1	rate					569:572	The herbicide release rate	547:572	The herbicide release rate from CMS/MMT composites in water	547:605	The herbicide release rate from CMS/MMT composites in water was significantly reduced when compared to commercial isoproturon: 95% released after ca. 700 h and ca. 24h, respectively.
26572334	4	18	theme	CMS/MMT	579:585	arg1	composites					587:596	CMS/MMT composites	579:596	CMS/MMT composites	579:596	The herbicide release rate from CMS/MMT composites in water was significantly reduced when compared to commercial isoproturon: 95% released after ca. 700 h and ca. 24h, respectively.
26572334	2	19	theme	native	345:350	arg1	starch					359:364	native potato starch	345:364	native potato starch	345:364	For preparing nanocomposite granules carboxymethyl starch (CMS) from native potato starch as well as CMS from amylopectin has been used.
26572334	2	20	from	amylopectin	386:396	arg1	CMS					335:337	CMS	335:337	CMS	335:337	For preparing nanocomposite granules carboxymethyl starch (CMS) from native potato starch as well as CMS from amylopectin has been used.
26572334	2	20	from	amylopectin	386:396	arg1	starch					327:332	carboxymethyl starch	313:332	carboxymethyl starch (CMS) from native potato starch as well as CMS from amylopectin	313:396	For preparing nanocomposite granules carboxymethyl starch (CMS) from native potato starch as well as CMS from amylopectin has been used.
26572334	2	20	from	amylopectin	386:396	arg1	CMS					377:379	CMS	377:379	carboxymethyl starch (CMS) from native potato starch as well as CMS from amylopectin	313:396	For preparing nanocomposite granules carboxymethyl starch (CMS) from native potato starch as well as CMS from amylopectin has been used.
26572334	1	21	theme	novel	128:132	arg1	microparticles					178:191	novel high substituted carboxymethyl starch-based microparticles	128:191	novel high substituted carboxymethyl starch-based microparticles containing sodium montmorillonite (MMT)	128:231	Preparation of novel high substituted carboxymethyl starch-based microparticles containing sodium montmorillonite (MMT) by crosslinking with Al(3+) was described.
26572334	3	22	theme	isoproturon	485:495	arg1	herbicide					469:477	herbicide	469:477	herbicide	469:477	The hydrophilic CMS/MMT composite systems were used for herbicide, i.e. isoproturon encapsulation (ca. 75% encapsulation efficiency).
26572334	3	22	theme	isoproturon	485:495	arg1	encapsulation					497:509	i.e. isoproturon encapsulation	480:509	i.e. isoproturon encapsulation (ca. 75% encapsulation efficiency)	480:544	The hydrophilic CMS/MMT composite systems were used for herbicide, i.e. isoproturon encapsulation (ca. 75% encapsulation efficiency).
26572334	0	23	theme	composite	37:45	arg1	microparticles					47:60	composite microparticles	37:60	composite microparticles: Properties and controlled release of isoproturon	37:110	Carboxymethyl starch/montmorillonite composite microparticles: Properties and controlled release of isoproturon.
26572334	1	24	theme	high	134:137	arg1	microparticles					178:191	novel high substituted carboxymethyl starch-based microparticles	128:191	novel high substituted carboxymethyl starch-based microparticles containing sodium montmorillonite (MMT)	128:231	Preparation of novel high substituted carboxymethyl starch-based microparticles containing sodium montmorillonite (MMT) by crosslinking with Al(3+) was described.
26572334	4	25	theme	isoproturon	661:671	arg1	%					676:676	commercial isoproturon: 95%	650:676	commercial isoproturon: 95% released after ca. 700 h	650:701	The herbicide release rate from CMS/MMT composites in water was significantly reduced when compared to commercial isoproturon: 95% released after ca. 700 h and ca. 24h, respectively.
26572334	3	26	theme	composite	437:445	arg1	systems					447:453	The hydrophilic CMS/MMT composite systems	413:453	The hydrophilic CMS/MMT composite systems	413:453	The hydrophilic CMS/MMT composite systems were used for herbicide, i.e. isoproturon encapsulation (ca. 75% encapsulation efficiency).
26572334	3	27	theme	%	518:518	arg1	efficiency					534:543	75% encapsulation efficiency	516:543	75% encapsulation efficiency	516:543	The hydrophilic CMS/MMT composite systems were used for herbicide, i.e. isoproturon encapsulation (ca. 75% encapsulation efficiency).
26572334	4	28	theme	herbicide	551:559	arg1	rate					569:572	The herbicide release rate	547:572	The herbicide release rate from CMS/MMT composites in water	547:605	The herbicide release rate from CMS/MMT composites in water was significantly reduced when compared to commercial isoproturon: 95% released after ca. 700 h and ca. 24h, respectively.
26572334	3	29	theme	i.e.	480:483	arg1	herbicide					469:477	herbicide	469:477	herbicide	469:477	The hydrophilic CMS/MMT composite systems were used for herbicide, i.e. isoproturon encapsulation (ca. 75% encapsulation efficiency).
26572334	3	29	theme	i.e.	480:483	arg1	encapsulation					497:509	i.e. isoproturon encapsulation	480:509	i.e. isoproturon encapsulation (ca. 75% encapsulation efficiency)	480:544	The hydrophilic CMS/MMT composite systems were used for herbicide, i.e. isoproturon encapsulation (ca. 75% encapsulation efficiency).
26572334	0	30	theme	controlled	78:87	arg1	release					89:95	controlled release	78:95	controlled release of isoproturon	78:110	Carboxymethyl starch/montmorillonite composite microparticles: Properties and controlled release of isoproturon.
26572334	6	31	theme	irrigations	839:849	arg1	series					823:828	a series	821:828	a series of eight irrigations	821:849	After a series of eight irrigations leached about 10% of isoproturon loaded.
26572334	1	32	theme	substituted	139:149	arg1	microparticles					178:191	novel high substituted carboxymethyl starch-based microparticles	128:191	novel high substituted carboxymethyl starch-based microparticles containing sodium montmorillonite (MMT)	128:231	Preparation of novel high substituted carboxymethyl starch-based microparticles containing sodium montmorillonite (MMT) by crosslinking with Al(3+) was described.
26572334	3	33	theme	CMS/MMT	429:435	arg1	systems					447:453	The hydrophilic CMS/MMT composite systems	413:453	The hydrophilic CMS/MMT composite systems	413:453	The hydrophilic CMS/MMT composite systems were used for herbicide, i.e. isoproturon encapsulation (ca. 75% encapsulation efficiency).
26572334	1	34	with	crosslinking	236:247	arg1	Al					254:255	Al	254:255	Al(3+)	254:259	Preparation of novel high substituted carboxymethyl starch-based microparticles containing sodium montmorillonite (MMT) by crosslinking with Al(3+) was described.
26572334	1	34	with	crosslinking	236:247	arg1	3+					257:258	3+	257:258	3+	257:258	Preparation of novel high substituted carboxymethyl starch-based microparticles containing sodium montmorillonite (MMT) by crosslinking with Al(3+) was described.
26572334	3	35	dep	efficiency	534:543	arg1	ca.					512:514	ca.	512:514	ca.	512:514	The hydrophilic CMS/MMT composite systems were used for herbicide, i.e. isoproturon encapsulation (ca. 75% encapsulation efficiency).
26572334	1	36	theme	carboxymethyl	151:163	arg1	microparticles					178:191	novel high substituted carboxymethyl starch-based microparticles	128:191	novel high substituted carboxymethyl starch-based microparticles containing sodium montmorillonite (MMT)	128:231	Preparation of novel high substituted carboxymethyl starch-based microparticles containing sodium montmorillonite (MMT) by crosslinking with Al(3+) was described.
26572334	4	37	theme	release	561:567	arg1	rate					569:572	The herbicide release rate	547:572	The herbicide release rate from CMS/MMT composites in water	547:605	The herbicide release rate from CMS/MMT composites in water was significantly reduced when compared to commercial isoproturon: 95% released after ca. 700 h and ca. 24h, respectively.
26572334	4	38	dep	700	697:699	arg1	ca.					693:695	ca.	693:695	ca.	693:695	The herbicide release rate from CMS/MMT composites in water was significantly reduced when compared to commercial isoproturon: 95% released after ca. 700 h and ca. 24h, respectively.
26572334	0	39	dep	microparticles	47:60	arg1	Properties					63:72	Properties	63:72	Properties	63:72	Carboxymethyl starch/montmorillonite composite microparticles: Properties and controlled release of isoproturon.
26572334	0	39	dep	microparticles	47:60	arg1	release					89:95	controlled release	78:95	controlled release of isoproturon	78:110	Carboxymethyl starch/montmorillonite composite microparticles: Properties and controlled release of isoproturon.
26572334	1	40	theme	starch-based	165:176	arg1	microparticles					178:191	novel high substituted carboxymethyl starch-based microparticles	128:191	novel high substituted carboxymethyl starch-based microparticles containing sodium montmorillonite (MMT)	128:231	Preparation of novel high substituted carboxymethyl starch-based microparticles containing sodium montmorillonite (MMT) by crosslinking with Al(3+) was described.
26572334	5	41	from	systems	762:768	arg1	Leaching					730:737	Leaching	730:737	Leaching in soil from composite systems	730:768	Leaching in soil from composite systems was relatively slower than release in water.
26572334	2	42	theme	nanocomposite	290:302	arg1	granules					304:311	nanocomposite granules	290:311	nanocomposite granules	290:311	For preparing nanocomposite granules carboxymethyl starch (CMS) from native potato starch as well as CMS from amylopectin has been used.
26572334	1	43	theme	microparticles	178:191	arg1	Preparation					113:123	Preparation	113:123	Preparation of novel high substituted carboxymethyl starch-based microparticles containing sodium montmorillonite (MMT) by crosslinking with Al(3+)	113:259	Preparation of novel high substituted carboxymethyl starch-based microparticles containing sodium montmorillonite (MMT) by crosslinking with Al(3+) was described.
26572334	7	44	theme	CMS/MMT	896:902	arg1	carriers					904:911	The CMS/MMT carriers	892:911	The CMS/MMT carriers	892:911	The CMS/MMT carriers could reduce the potential leaching of herbicide and beneficially reduce pollution of the environment.
26572334	2	45	used	used	407:410	arg2	CMS					335:337	CMS	335:337	CMS	335:337	For preparing nanocomposite granules carboxymethyl starch (CMS) from native potato starch as well as CMS from amylopectin has been used.
26572334	2	45	used	used	407:410	arg2	CMS					377:379	CMS	377:379	carboxymethyl starch (CMS) from native potato starch as well as CMS from amylopectin	313:396	For preparing nanocomposite granules carboxymethyl starch (CMS) from native potato starch as well as CMS from amylopectin has been used.
26572334	2	45	used	used	407:410	arg2	starch					327:332	carboxymethyl starch	313:332	carboxymethyl starch (CMS) from native potato starch as well as CMS from amylopectin	313:396	For preparing nanocomposite granules carboxymethyl starch (CMS) from native potato starch as well as CMS from amylopectin has been used.
26572334	4	46	theme	commercial	650:659	arg1	%					676:676	commercial isoproturon: 95%	650:676	commercial isoproturon: 95% released after ca. 700 h	650:701	The herbicide release rate from CMS/MMT composites in water was significantly reduced when compared to commercial isoproturon: 95% released after ca. 700 h and ca. 24h, respectively.
26572334	3	47	theme	hydrophilic	417:427	arg1	systems					447:453	The hydrophilic CMS/MMT composite systems	413:453	The hydrophilic CMS/MMT composite systems	413:453	The hydrophilic CMS/MMT composite systems were used for herbicide, i.e. isoproturon encapsulation (ca. 75% encapsulation efficiency).
26572334	1	48	theme	sodium	204:209	arg1	MMT					228:230	MMT	228:230	MMT	228:230	Preparation of novel high substituted carboxymethyl starch-based microparticles containing sodium montmorillonite (MMT) by crosslinking with Al(3+) was described.
26572334	1	48	theme	sodium	204:209	arg1	montmorillonite					211:225	sodium montmorillonite	204:225	sodium montmorillonite (MMT)	204:231	Preparation of novel high substituted carboxymethyl starch-based microparticles containing sodium montmorillonite (MMT) by crosslinking with Al(3+) was described.
27395072	5	0	theme	chemical	1123:1130	arg1	extractions					1132:1142	the three composite chemical extractions	1103:1142	the three composite chemical extractions (HPCD/AS, HPCD/RL, HPCD/BSA)	1103:1171	However, the overall performances of the three composite chemical extractions (HPCD/AS, HPCD/RL, HPCD/BSA) were better than individual HPCD extraction, among which, HPCD/AS was best at estimating the earthworm accumulation (considering both correlation coefficient and fitted linear slope).
27395072	2	1	theme	serum	589:593	arg1	BSA					604:606	BSA	604:606	BSA	604:606	In this study, a novel technique, composite extraction of HPCD each with rhamnolipid (RL), bovine serum albumin (BSA) and alginate sodium (AS), models of lipopolysaccharide, proteins and polysaccharides, was developed to assess PAHs bioaccessibility to earthworms.
27395072	2	1	theme	serum	589:593	arg1	albumin					595:601	bovine serum albumin	582:601	bovine serum albumin (BSA)	582:607	In this study, a novel technique, composite extraction of HPCD each with rhamnolipid (RL), bovine serum albumin (BSA) and alginate sodium (AS), models of lipopolysaccharide, proteins and polysaccharides, was developed to assess PAHs bioaccessibility to earthworms.
27395072	0	2	from	method	36:41	arg1	soil					55:58	soil	55:58	soil	55:58	A novel bioaccessibility prediction method for PAHs in soil: Composite extraction with hydroxypropyl-β-cyclodextrin and extracellular polymer substances.
27395072	3	3	theme	HPCD	853:856	arg1	extraction					858:867	individual HPCD extraction	842:867	individual HPCD extraction	842:867	In addition, comparisons were conducted with accelerated solvent extraction (ASE) and individual HPCD extraction.
27395072	0	4	theme	Composite	61:69	arg1	extraction					71:80	Composite extraction	61:80	A novel bioaccessibility prediction method for PAHs in soil: Composite extraction with hydroxypropyl-β-cyclodextrin and extracellular polymer substances.	0:152	A novel bioaccessibility prediction method for PAHs in soil: Composite extraction with hydroxypropyl-β-cyclodextrin and extracellular polymer substances.
27395072	5	5	theme	extractions	1132:1142	arg1	performances					1087:1098	the overall performances	1075:1098	the overall performances of the three composite chemical extractions (HPCD/AS, HPCD/RL, HPCD/BSA)	1075:1171	However, the overall performances of the three composite chemical extractions (HPCD/AS, HPCD/RL, HPCD/BSA) were better than individual HPCD extraction, among which, HPCD/AS was best at estimating the earthworm accumulation (considering both correlation coefficient and fitted linear slope).
27395072	5	5	theme	extractions	1132:1142	arg1	better					1178:1183	better	1178:1183	better	1178:1183	However, the overall performances of the three composite chemical extractions (HPCD/AS, HPCD/RL, HPCD/BSA) were better than individual HPCD extraction, among which, HPCD/AS was best at estimating the earthworm accumulation (considering both correlation coefficient and fitted linear slope).
27395072	2	6	theme	bovine	582:587	arg1	BSA					604:606	BSA	604:606	BSA	604:606	In this study, a novel technique, composite extraction of HPCD each with rhamnolipid (RL), bovine serum albumin (BSA) and alginate sodium (AS), models of lipopolysaccharide, proteins and polysaccharides, was developed to assess PAHs bioaccessibility to earthworms.
27395072	2	6	theme	bovine	582:587	arg1	albumin					595:601	bovine serum albumin	582:601	bovine serum albumin (BSA)	582:607	In this study, a novel technique, composite extraction of HPCD each with rhamnolipid (RL), bovine serum albumin (BSA) and alginate sodium (AS), models of lipopolysaccharide, proteins and polysaccharides, was developed to assess PAHs bioaccessibility to earthworms.
27395072	0	7	with	extraction	71:80	arg1	hydroxypropyl-β-cyclodextrin					87:114	hydroxypropyl-β-cyclodextrin	87:114	hydroxypropyl-β-cyclodextrin	87:114	A novel bioaccessibility prediction method for PAHs in soil: Composite extraction with hydroxypropyl-β-cyclodextrin and extracellular polymer substances.
27395072	0	7	with	extraction	71:80	arg1	substances					142:151	extracellular polymer substances	120:151	extracellular polymer substances	120:151	A novel bioaccessibility prediction method for PAHs in soil: Composite extraction with hydroxypropyl-β-cyclodextrin and extracellular polymer substances.
27395072	3	8	theme	accelerated	801:811	arg1	ASE					833:835	ASE	833:835	ASE	833:835	In addition, comparisons were conducted with accelerated solvent extraction (ASE) and individual HPCD extraction.
27395072	3	8	theme	accelerated	801:811	arg1	extraction					821:830	accelerated solvent extraction	801:830	accelerated solvent extraction (ASE)	801:836	In addition, comparisons were conducted with accelerated solvent extraction (ASE) and individual HPCD extraction.
27395072	2	9	theme	composite	525:533	arg1	technique					514:522	a novel technique	506:522	a novel technique	506:522	In this study, a novel technique, composite extraction of HPCD each with rhamnolipid (RL), bovine serum albumin (BSA) and alginate sodium (AS), models of lipopolysaccharide, proteins and polysaccharides, was developed to assess PAHs bioaccessibility to earthworms.
27395072	2	9	theme	composite	525:533	arg1	extraction					535:544	composite extraction	525:544	composite extraction of HPCD each with rhamnolipid (RL), bovine serum albumin (BSA) and alginate sodium (AS), models of lipopolysaccharide, proteins and polysaccharides	525:692	In this study, a novel technique, composite extraction of HPCD each with rhamnolipid (RL), bovine serum albumin (BSA) and alginate sodium (AS), models of lipopolysaccharide, proteins and polysaccharides, was developed to assess PAHs bioaccessibility to earthworms.
27395072	5	10	dep	accumulation	1276:1287	arg1	considering					1290:1300	considering	1290:1300	considering both correlation coefficient and fitted linear slope	1290:1353	However, the overall performances of the three composite chemical extractions (HPCD/AS, HPCD/RL, HPCD/BSA) were better than individual HPCD extraction, among which, HPCD/AS was best at estimating the earthworm accumulation (considering both correlation coefficient and fitted linear slope).
27395072	6	11	used	used	1426:1429	arg2	method					1454:1459	a fast and reliable method	1434:1459	a fast and reliable method to predict PAH bioaccessibility to earthworms in contaminated soils	1434:1527	Therefore, all observations implied that HPCD/AS extraction could be used as a fast and reliable method to predict PAH bioaccessibility to earthworms in contaminated soils.
27395072	6	11	used	used	1426:1429	arg2	extraction					1406:1415	HPCD/AS extraction	1398:1415	HPCD/AS extraction	1398:1415	Therefore, all observations implied that HPCD/AS extraction could be used as a fast and reliable method to predict PAH bioaccessibility to earthworms in contaminated soils.
27395072	2	12	dep	sodium	622:627	arg1	AS					630:631	AS	630:631	AS	630:631	In this study, a novel technique, composite extraction of HPCD each with rhamnolipid (RL), bovine serum albumin (BSA) and alginate sodium (AS), models of lipopolysaccharide, proteins and polysaccharides, was developed to assess PAHs bioaccessibility to earthworms.
27395072	5	13	theme	HPCD	1201:1204	arg1	extraction					1206:1215	individual HPCD extraction	1190:1215	individual HPCD extraction	1190:1215	However, the overall performances of the three composite chemical extractions (HPCD/AS, HPCD/RL, HPCD/BSA) were better than individual HPCD extraction, among which, HPCD/AS was best at estimating the earthworm accumulation (considering both correlation coefficient and fitted linear slope).
27395072	1	14	theme	information	362:372	arg1	lack					354:357	lack	354:357	lack of information regarding the exogenous active substances, such as extracellular polymer substance (EPS) secreted by microorganisms	354:488	Hydroxypropyl-β-cyclodextrin (HPCD) extraction has been widely used to estimate bioaccessibility of polycyclic aromatic hydrocarbons (PAHs) in soil, but it often underestimates the actual risk due to lack of information regarding the exogenous active substances, such as extracellular polymer substance (EPS) secreted by microorganisms.
27395072	4	15	theme	PAH	1025:1027	arg1	bioaccessibility					1029:1044	PAH bioaccessibility	1025:1044	PAH bioaccessibility	1025:1044	The results demonstrated that all chemical extractions were linearly correlated with earthworm accumulation, and individual HPCD extraction underestimated PAH bioaccessibility by about two times.
27395072	2	16	theme	alginate	613:620	arg1	sodium					622:627	alginate sodium	613:627	alginate sodium (AS)	613:632	In this study, a novel technique, composite extraction of HPCD each with rhamnolipid (RL), bovine serum albumin (BSA) and alginate sodium (AS), models of lipopolysaccharide, proteins and polysaccharides, was developed to assess PAHs bioaccessibility to earthworms.
27395072	0	17	theme	extracellular	120:132	arg1	substances					142:151	extracellular polymer substances	120:151	extracellular polymer substances	120:151	A novel bioaccessibility prediction method for PAHs in soil: Composite extraction with hydroxypropyl-β-cyclodextrin and extracellular polymer substances.
27395072	2	18	theme	HPCD	549:552	arg1	technique					514:522	a novel technique	506:522	a novel technique	506:522	In this study, a novel technique, composite extraction of HPCD each with rhamnolipid (RL), bovine serum albumin (BSA) and alginate sodium (AS), models of lipopolysaccharide, proteins and polysaccharides, was developed to assess PAHs bioaccessibility to earthworms.
27395072	2	18	theme	HPCD	549:552	arg1	extraction					535:544	composite extraction	525:544	composite extraction of HPCD each with rhamnolipid (RL), bovine serum albumin (BSA) and alginate sodium (AS), models of lipopolysaccharide, proteins and polysaccharides	525:692	In this study, a novel technique, composite extraction of HPCD each with rhamnolipid (RL), bovine serum albumin (BSA) and alginate sodium (AS), models of lipopolysaccharide, proteins and polysaccharides, was developed to assess PAHs bioaccessibility to earthworms.
27395072	6	19	theme	reliable	1445:1452	arg1	extraction					1406:1415	HPCD/AS extraction	1398:1415	HPCD/AS extraction	1398:1415	Therefore, all observations implied that HPCD/AS extraction could be used as a fast and reliable method to predict PAH bioaccessibility to earthworms in contaminated soils.
27395072	6	19	theme	reliable	1445:1452	arg1	method					1454:1459	a fast and reliable method	1434:1459	a fast and reliable method to predict PAH bioaccessibility to earthworms in contaminated soils	1434:1527	Therefore, all observations implied that HPCD/AS extraction could be used as a fast and reliable method to predict PAH bioaccessibility to earthworms in contaminated soils.
27395072	5	20	theme	earthworm	1266:1274	arg1	accumulation					1276:1287	the earthworm accumulation	1262:1287	the earthworm accumulation (considering both correlation coefficient and fitted linear slope)	1262:1354	However, the overall performances of the three composite chemical extractions (HPCD/AS, HPCD/RL, HPCD/BSA) were better than individual HPCD extraction, among which, HPCD/AS was best at estimating the earthworm accumulation (considering both correlation coefficient and fitted linear slope).
27395072	2	21	theme	novel	508:512	arg1	technique					514:522	a novel technique	506:522	a novel technique	506:522	In this study, a novel technique, composite extraction of HPCD each with rhamnolipid (RL), bovine serum albumin (BSA) and alginate sodium (AS), models of lipopolysaccharide, proteins and polysaccharides, was developed to assess PAHs bioaccessibility to earthworms.
27395072	2	21	theme	novel	508:512	arg1	extraction					535:544	composite extraction	525:544	composite extraction of HPCD each with rhamnolipid (RL), bovine serum albumin (BSA) and alginate sodium (AS), models of lipopolysaccharide, proteins and polysaccharides	525:692	In this study, a novel technique, composite extraction of HPCD each with rhamnolipid (RL), bovine serum albumin (BSA) and alginate sodium (AS), models of lipopolysaccharide, proteins and polysaccharides, was developed to assess PAHs bioaccessibility to earthworms.
27395072	2	22	theme	models	635:640	arg1	technique					514:522	a novel technique	506:522	a novel technique	506:522	In this study, a novel technique, composite extraction of HPCD each with rhamnolipid (RL), bovine serum albumin (BSA) and alginate sodium (AS), models of lipopolysaccharide, proteins and polysaccharides, was developed to assess PAHs bioaccessibility to earthworms.
27395072	2	22	theme	models	635:640	arg1	extraction					535:544	composite extraction	525:544	composite extraction of HPCD each with rhamnolipid (RL), bovine serum albumin (BSA) and alginate sodium (AS), models of lipopolysaccharide, proteins and polysaccharides	525:692	In this study, a novel technique, composite extraction of HPCD each with rhamnolipid (RL), bovine serum albumin (BSA) and alginate sodium (AS), models of lipopolysaccharide, proteins and polysaccharides, was developed to assess PAHs bioaccessibility to earthworms.
27395072	5	23	theme	fitted	1335:1340	arg1	slope					1349:1353	fitted linear slope	1335:1353	fitted linear slope	1335:1353	However, the overall performances of the three composite chemical extractions (HPCD/AS, HPCD/RL, HPCD/BSA) were better than individual HPCD extraction, among which, HPCD/AS was best at estimating the earthworm accumulation (considering both correlation coefficient and fitted linear slope).
27395072	0	24	theme	novel	2:6	arg1	method					36:41	A novel bioaccessibility prediction method	0:41	A novel bioaccessibility prediction method for PAHs in soil: Composite extraction with hydroxypropyl-β-cyclodextrin and extracellular polymer substances.	0:152	A novel bioaccessibility prediction method for PAHs in soil: Composite extraction with hydroxypropyl-β-cyclodextrin and extracellular polymer substances.
27395072	1	25	theme	exogenous	388:396	arg1	substances					405:414	the exogenous active substances	384:414	the exogenous active substances	384:414	Hydroxypropyl-β-cyclodextrin (HPCD) extraction has been widely used to estimate bioaccessibility of polycyclic aromatic hydrocarbons (PAHs) in soil, but it often underestimates the actual risk due to lack of information regarding the exogenous active substances, such as extracellular polymer substance (EPS) secreted by microorganisms.
27395072	1	25	theme	exogenous	388:396	arg1	substance					447:455	extracellular polymer substance	425:455	extracellular polymer substance (EPS) secreted by microorganisms	425:488	Hydroxypropyl-β-cyclodextrin (HPCD) extraction has been widely used to estimate bioaccessibility of polycyclic aromatic hydrocarbons (PAHs) in soil, but it often underestimates the actual risk due to lack of information regarding the exogenous active substances, such as extracellular polymer substance (EPS) secreted by microorganisms.
27395072	6	26	from	earthworms	1496:1505	arg1	soils					1523:1527	contaminated soils	1510:1527	contaminated soils	1510:1527	Therefore, all observations implied that HPCD/AS extraction could be used as a fast and reliable method to predict PAH bioaccessibility to earthworms in contaminated soils.
27395072	5	27	theme	linear	1342:1347	arg1	slope					1349:1353	fitted linear slope	1335:1353	fitted linear slope	1335:1353	However, the overall performances of the three composite chemical extractions (HPCD/AS, HPCD/RL, HPCD/BSA) were better than individual HPCD extraction, among which, HPCD/AS was best at estimating the earthworm accumulation (considering both correlation coefficient and fitted linear slope).
27395072	5	28	dep	extractions	1132:1142	arg1	HPCD/RL					1154:1160	HPCD/RL	1154:1160	HPCD/RL	1154:1160	However, the overall performances of the three composite chemical extractions (HPCD/AS, HPCD/RL, HPCD/BSA) were better than individual HPCD extraction, among which, HPCD/AS was best at estimating the earthworm accumulation (considering both correlation coefficient and fitted linear slope).
27395072	5	28	dep	extractions	1132:1142	arg1	HPCD/AS					1145:1151	HPCD/AS	1145:1151	HPCD/AS	1145:1151	However, the overall performances of the three composite chemical extractions (HPCD/AS, HPCD/RL, HPCD/BSA) were better than individual HPCD extraction, among which, HPCD/AS was best at estimating the earthworm accumulation (considering both correlation coefficient and fitted linear slope).
27395072	5	28	dep	extractions	1132:1142	arg1	HPCD/BSA					1163:1170	HPCD/BSA	1163:1170	HPCD/BSA	1163:1170	However, the overall performances of the three composite chemical extractions (HPCD/AS, HPCD/RL, HPCD/BSA) were better than individual HPCD extraction, among which, HPCD/AS was best at estimating the earthworm accumulation (considering both correlation coefficient and fitted linear slope).
27395072	6	29	theme	contaminated	1510:1521	arg1	soils					1523:1527	contaminated soils	1510:1527	contaminated soils	1510:1527	Therefore, all observations implied that HPCD/AS extraction could be used as a fast and reliable method to predict PAH bioaccessibility to earthworms in contaminated soils.
27395072	0	30	theme	prediction	25:34	arg1	method					36:41	A novel bioaccessibility prediction method	0:41	A novel bioaccessibility prediction method for PAHs in soil: Composite extraction with hydroxypropyl-β-cyclodextrin and extracellular polymer substances.	0:152	A novel bioaccessibility prediction method for PAHs in soil: Composite extraction with hydroxypropyl-β-cyclodextrin and extracellular polymer substances.
27395072	0	31	theme	bioaccessibility	8:23	arg1	method					36:41	A novel bioaccessibility prediction method	0:41	A novel bioaccessibility prediction method for PAHs in soil: Composite extraction with hydroxypropyl-β-cyclodextrin and extracellular polymer substances.	0:152	A novel bioaccessibility prediction method for PAHs in soil: Composite extraction with hydroxypropyl-β-cyclodextrin and extracellular polymer substances.
27395072	0	32	theme	polymer	134:140	arg1	substances					142:151	extracellular polymer substances	120:151	extracellular polymer substances	120:151	A novel bioaccessibility prediction method for PAHs in soil: Composite extraction with hydroxypropyl-β-cyclodextrin and extracellular polymer substances.
27395072	1	33	theme	active	398:403	arg1	substances					405:414	the exogenous active substances	384:414	the exogenous active substances	384:414	Hydroxypropyl-β-cyclodextrin (HPCD) extraction has been widely used to estimate bioaccessibility of polycyclic aromatic hydrocarbons (PAHs) in soil, but it often underestimates the actual risk due to lack of information regarding the exogenous active substances, such as extracellular polymer substance (EPS) secreted by microorganisms.
27395072	1	33	theme	active	398:403	arg1	substance					447:455	extracellular polymer substance	425:455	extracellular polymer substance (EPS) secreted by microorganisms	425:488	Hydroxypropyl-β-cyclodextrin (HPCD) extraction has been widely used to estimate bioaccessibility of polycyclic aromatic hydrocarbons (PAHs) in soil, but it often underestimates the actual risk due to lack of information regarding the exogenous active substances, such as extracellular polymer substance (EPS) secreted by microorganisms.
27395072	5	34	theme	correlation	1307:1317	arg1	coefficient					1319:1329	correlation coefficient	1307:1329	correlation coefficient	1307:1329	However, the overall performances of the three composite chemical extractions (HPCD/AS, HPCD/RL, HPCD/BSA) were better than individual HPCD extraction, among which, HPCD/AS was best at estimating the earthworm accumulation (considering both correlation coefficient and fitted linear slope).
27395072	4	35	theme	individual	983:992	arg1	extraction					999:1008	individual HPCD extraction	983:1008	individual HPCD extraction	983:1008	The results demonstrated that all chemical extractions were linearly correlated with earthworm accumulation, and individual HPCD extraction underestimated PAH bioaccessibility by about two times.
27395072	4	36	theme	chemical	904:911	arg1	extractions					913:923	all chemical extractions	900:923	all chemical extractions	900:923	The results demonstrated that all chemical extractions were linearly correlated with earthworm accumulation, and individual HPCD extraction underestimated PAH bioaccessibility by about two times.
27395072	2	37	theme	bioaccessibility	724:739	arg1	PAHs					719:722	PAHs	719:722	PAHs bioaccessibility to earthworms	719:753	In this study, a novel technique, composite extraction of HPCD each with rhamnolipid (RL), bovine serum albumin (BSA) and alginate sodium (AS), models of lipopolysaccharide, proteins and polysaccharides, was developed to assess PAHs bioaccessibility to earthworms.
27395072	5	38	theme	individual	1190:1199	arg1	extraction					1206:1215	individual HPCD extraction	1190:1215	individual HPCD extraction	1190:1215	However, the overall performances of the three composite chemical extractions (HPCD/AS, HPCD/RL, HPCD/BSA) were better than individual HPCD extraction, among which, HPCD/AS was best at estimating the earthworm accumulation (considering both correlation coefficient and fitted linear slope).
27395072	1	39	from	bioaccessibility	234:249	arg1	soil					297:300	soil	297:300	soil	297:300	Hydroxypropyl-β-cyclodextrin (HPCD) extraction has been widely used to estimate bioaccessibility of polycyclic aromatic hydrocarbons (PAHs) in soil, but it often underestimates the actual risk due to lack of information regarding the exogenous active substances, such as extracellular polymer substance (EPS) secreted by microorganisms.
27395072	2	40	dep	HPCD	549:552	arg1	each					554:557	each	554:557	each	554:557	In this study, a novel technique, composite extraction of HPCD each with rhamnolipid (RL), bovine serum albumin (BSA) and alginate sodium (AS), models of lipopolysaccharide, proteins and polysaccharides, was developed to assess PAHs bioaccessibility to earthworms.
27395072	6	41	theme	HPCD/AS	1398:1404	arg1	extraction					1406:1415	HPCD/AS extraction	1398:1415	HPCD/AS extraction	1398:1415	Therefore, all observations implied that HPCD/AS extraction could be used as a fast and reliable method to predict PAH bioaccessibility to earthworms in contaminated soils.
27395072	6	41	theme	HPCD/AS	1398:1404	arg1	method					1454:1459	a fast and reliable method	1434:1459	a fast and reliable method to predict PAH bioaccessibility to earthworms in contaminated soils	1434:1527	Therefore, all observations implied that HPCD/AS extraction could be used as a fast and reliable method to predict PAH bioaccessibility to earthworms in contaminated soils.
27395072	5	42	theme	overall	1079:1085	arg1	performances					1087:1098	the overall performances	1075:1098	the overall performances of the three composite chemical extractions (HPCD/AS, HPCD/RL, HPCD/BSA)	1075:1171	However, the overall performances of the three composite chemical extractions (HPCD/AS, HPCD/RL, HPCD/BSA) were better than individual HPCD extraction, among which, HPCD/AS was best at estimating the earthworm accumulation (considering both correlation coefficient and fitted linear slope).
27395072	5	42	theme	overall	1079:1085	arg1	better					1178:1183	better	1178:1183	better	1178:1183	However, the overall performances of the three composite chemical extractions (HPCD/AS, HPCD/RL, HPCD/BSA) were better than individual HPCD extraction, among which, HPCD/AS was best at estimating the earthworm accumulation (considering both correlation coefficient and fitted linear slope).
27395072	4	43	theme	HPCD	994:997	arg1	extraction					999:1008	individual HPCD extraction	983:1008	individual HPCD extraction	983:1008	The results demonstrated that all chemical extractions were linearly correlated with earthworm accumulation, and individual HPCD extraction underestimated PAH bioaccessibility by about two times.
27395072	1	44	theme	actual	335:340	arg1	risk					342:345	the actual risk	331:345	the actual risk due to lack of information regarding the exogenous active substances, such as extracellular polymer substance (EPS) secreted by microorganisms	331:488	Hydroxypropyl-β-cyclodextrin (HPCD) extraction has been widely used to estimate bioaccessibility of polycyclic aromatic hydrocarbons (PAHs) in soil, but it often underestimates the actual risk due to lack of information regarding the exogenous active substances, such as extracellular polymer substance (EPS) secreted by microorganisms.
27395072	5	45	theme	composite	1113:1121	arg1	extractions					1132:1142	the three composite chemical extractions	1103:1142	the three composite chemical extractions (HPCD/AS, HPCD/RL, HPCD/BSA)	1103:1171	However, the overall performances of the three composite chemical extractions (HPCD/AS, HPCD/RL, HPCD/BSA) were better than individual HPCD extraction, among which, HPCD/AS was best at estimating the earthworm accumulation (considering both correlation coefficient and fitted linear slope).
27395072	0	46	dep	method	36:41	arg1	extraction					71:80	Composite extraction	61:80	A novel bioaccessibility prediction method for PAHs in soil: Composite extraction with hydroxypropyl-β-cyclodextrin and extracellular polymer substances.	0:152	A novel bioaccessibility prediction method for PAHs in soil: Composite extraction with hydroxypropyl-β-cyclodextrin and extracellular polymer substances.
27395072	2	47	theme	lipopolysaccharide	645:662	arg1	HPCD					549:552	HPCD each with rhamnolipid (RL), bovine serum albumin (BSA) and alginate sodium (AS), models of lipopolysaccharide, proteins and polysaccharides	549:692	HPCD	549:552	In this study, a novel technique, composite extraction of HPCD each with rhamnolipid (RL), bovine serum albumin (BSA) and alginate sodium (AS), models of lipopolysaccharide, proteins and polysaccharides, was developed to assess PAHs bioaccessibility to earthworms.
27395072	2	47	theme	lipopolysaccharide	645:662	arg1	polysaccharides					678:692	polysaccharides	678:692	polysaccharides	678:692	In this study, a novel technique, composite extraction of HPCD each with rhamnolipid (RL), bovine serum albumin (BSA) and alginate sodium (AS), models of lipopolysaccharide, proteins and polysaccharides, was developed to assess PAHs bioaccessibility to earthworms.
27395072	2	47	theme	lipopolysaccharide	645:662	arg1	proteins					665:672	proteins	665:672	proteins	665:672	In this study, a novel technique, composite extraction of HPCD each with rhamnolipid (RL), bovine serum albumin (BSA) and alginate sodium (AS), models of lipopolysaccharide, proteins and polysaccharides, was developed to assess PAHs bioaccessibility to earthworms.
27395072	2	47	theme	lipopolysaccharide	645:662	arg1	models					635:640	models	635:640	models of lipopolysaccharide	635:662	In this study, a novel technique, composite extraction of HPCD each with rhamnolipid (RL), bovine serum albumin (BSA) and alginate sodium (AS), models of lipopolysaccharide, proteins and polysaccharides, was developed to assess PAHs bioaccessibility to earthworms.
27395072	1	48	theme	extracellular	425:437	arg1	EPS					458:460	EPS	458:460	EPS	458:460	Hydroxypropyl-β-cyclodextrin (HPCD) extraction has been widely used to estimate bioaccessibility of polycyclic aromatic hydrocarbons (PAHs) in soil, but it often underestimates the actual risk due to lack of information regarding the exogenous active substances, such as extracellular polymer substance (EPS) secreted by microorganisms.
27395072	1	48	theme	extracellular	425:437	arg1	substance					447:455	extracellular polymer substance	425:455	extracellular polymer substance (EPS) secreted by microorganisms	425:488	Hydroxypropyl-β-cyclodextrin (HPCD) extraction has been widely used to estimate bioaccessibility of polycyclic aromatic hydrocarbons (PAHs) in soil, but it often underestimates the actual risk due to lack of information regarding the exogenous active substances, such as extracellular polymer substance (EPS) secreted by microorganisms.
27395072	1	49	used	used	217:220	arg2	extraction					190:199	Hydroxypropyl-β-cyclodextrin (HPCD) extraction	154:199	Hydroxypropyl-β-cyclodextrin (HPCD) extraction	154:199	Hydroxypropyl-β-cyclodextrin (HPCD) extraction has been widely used to estimate bioaccessibility of polycyclic aromatic hydrocarbons (PAHs) in soil, but it often underestimates the actual risk due to lack of information regarding the exogenous active substances, such as extracellular polymer substance (EPS) secreted by microorganisms.
27395072	6	50	theme	PAH	1472:1474	arg1	bioaccessibility					1476:1491	PAH bioaccessibility	1472:1491	PAH bioaccessibility	1472:1491	Therefore, all observations implied that HPCD/AS extraction could be used as a fast and reliable method to predict PAH bioaccessibility to earthworms in contaminated soils.
27395072	1	51	theme	polycyclic	254:263	arg1	hydrocarbons					274:285	polycyclic aromatic hydrocarbons	254:285	polycyclic aromatic hydrocarbons (PAHs)	254:292	Hydroxypropyl-β-cyclodextrin (HPCD) extraction has been widely used to estimate bioaccessibility of polycyclic aromatic hydrocarbons (PAHs) in soil, but it often underestimates the actual risk due to lack of information regarding the exogenous active substances, such as extracellular polymer substance (EPS) secreted by microorganisms.
27395072	1	51	theme	polycyclic	254:263	arg1	PAHs					288:291	PAHs	288:291	PAHs	288:291	Hydroxypropyl-β-cyclodextrin (HPCD) extraction has been widely used to estimate bioaccessibility of polycyclic aromatic hydrocarbons (PAHs) in soil, but it often underestimates the actual risk due to lack of information regarding the exogenous active substances, such as extracellular polymer substance (EPS) secreted by microorganisms.
27395072	1	52	theme	polymer	439:445	arg1	EPS					458:460	EPS	458:460	EPS	458:460	Hydroxypropyl-β-cyclodextrin (HPCD) extraction has been widely used to estimate bioaccessibility of polycyclic aromatic hydrocarbons (PAHs) in soil, but it often underestimates the actual risk due to lack of information regarding the exogenous active substances, such as extracellular polymer substance (EPS) secreted by microorganisms.
27395072	1	52	theme	polymer	439:445	arg1	substance					447:455	extracellular polymer substance	425:455	extracellular polymer substance (EPS) secreted by microorganisms	425:488	Hydroxypropyl-β-cyclodextrin (HPCD) extraction has been widely used to estimate bioaccessibility of polycyclic aromatic hydrocarbons (PAHs) in soil, but it often underestimates the actual risk due to lack of information regarding the exogenous active substances, such as extracellular polymer substance (EPS) secreted by microorganisms.
27395072	2	53	theme	proteins	665:672	arg1	technique					514:522	a novel technique	506:522	a novel technique	506:522	In this study, a novel technique, composite extraction of HPCD each with rhamnolipid (RL), bovine serum albumin (BSA) and alginate sodium (AS), models of lipopolysaccharide, proteins and polysaccharides, was developed to assess PAHs bioaccessibility to earthworms.
27395072	2	53	theme	proteins	665:672	arg1	extraction					535:544	composite extraction	525:544	composite extraction of HPCD each with rhamnolipid (RL), bovine serum albumin (BSA) and alginate sodium (AS), models of lipopolysaccharide, proteins and polysaccharides	525:692	In this study, a novel technique, composite extraction of HPCD each with rhamnolipid (RL), bovine serum albumin (BSA) and alginate sodium (AS), models of lipopolysaccharide, proteins and polysaccharides, was developed to assess PAHs bioaccessibility to earthworms.
27395072	1	54	theme	Hydroxypropyl-β-cyclodextrin	154:181	arg1	extraction					190:199	Hydroxypropyl-β-cyclodextrin (HPCD) extraction	154:199	Hydroxypropyl-β-cyclodextrin (HPCD) extraction	154:199	Hydroxypropyl-β-cyclodextrin (HPCD) extraction has been widely used to estimate bioaccessibility of polycyclic aromatic hydrocarbons (PAHs) in soil, but it often underestimates the actual risk due to lack of information regarding the exogenous active substances, such as extracellular polymer substance (EPS) secreted by microorganisms.
27395072	3	55	theme	solvent	813:819	arg1	ASE					833:835	ASE	833:835	ASE	833:835	In addition, comparisons were conducted with accelerated solvent extraction (ASE) and individual HPCD extraction.
27395072	3	55	theme	solvent	813:819	arg1	extraction					821:830	accelerated solvent extraction	801:830	accelerated solvent extraction (ASE)	801:836	In addition, comparisons were conducted with accelerated solvent extraction (ASE) and individual HPCD extraction.
27395072	1	56	theme	aromatic	265:272	arg1	hydrocarbons					274:285	polycyclic aromatic hydrocarbons	254:285	polycyclic aromatic hydrocarbons (PAHs)	254:292	Hydroxypropyl-β-cyclodextrin (HPCD) extraction has been widely used to estimate bioaccessibility of polycyclic aromatic hydrocarbons (PAHs) in soil, but it often underestimates the actual risk due to lack of information regarding the exogenous active substances, such as extracellular polymer substance (EPS) secreted by microorganisms.
27395072	1	56	theme	aromatic	265:272	arg1	PAHs					288:291	PAHs	288:291	PAHs	288:291	Hydroxypropyl-β-cyclodextrin (HPCD) extraction has been widely used to estimate bioaccessibility of polycyclic aromatic hydrocarbons (PAHs) in soil, but it often underestimates the actual risk due to lack of information regarding the exogenous active substances, such as extracellular polymer substance (EPS) secreted by microorganisms.
27395072	4	57	theme	earthworm	955:963	arg1	accumulation					965:976	earthworm accumulation	955:976	earthworm accumulation	955:976	The results demonstrated that all chemical extractions were linearly correlated with earthworm accumulation, and individual HPCD extraction underestimated PAH bioaccessibility by about two times.
27395072	6	58	theme	fast	1436:1439	arg1	extraction					1406:1415	HPCD/AS extraction	1398:1415	HPCD/AS extraction	1398:1415	Therefore, all observations implied that HPCD/AS extraction could be used as a fast and reliable method to predict PAH bioaccessibility to earthworms in contaminated soils.
27395072	6	58	theme	fast	1436:1439	arg1	method					1454:1459	a fast and reliable method	1434:1459	a fast and reliable method to predict PAH bioaccessibility to earthworms in contaminated soils	1434:1527	Therefore, all observations implied that HPCD/AS extraction could be used as a fast and reliable method to predict PAH bioaccessibility to earthworms in contaminated soils.
27395072	1	59	theme	hydrocarbons	274:285	arg1	bioaccessibility					234:249	bioaccessibility	234:249	bioaccessibility of polycyclic aromatic hydrocarbons (PAHs) in soil	234:300	Hydroxypropyl-β-cyclodextrin (HPCD) extraction has been widely used to estimate bioaccessibility of polycyclic aromatic hydrocarbons (PAHs) in soil, but it often underestimates the actual risk due to lack of information regarding the exogenous active substances, such as extracellular polymer substance (EPS) secreted by microorganisms.
27395072	1	60	theme	due	347:349	arg1	risk					342:345	the actual risk	331:345	the actual risk due to lack of information regarding the exogenous active substances, such as extracellular polymer substance (EPS) secreted by microorganisms	331:488	Hydroxypropyl-β-cyclodextrin (HPCD) extraction has been widely used to estimate bioaccessibility of polycyclic aromatic hydrocarbons (PAHs) in soil, but it often underestimates the actual risk due to lack of information regarding the exogenous active substances, such as extracellular polymer substance (EPS) secreted by microorganisms.
27395072	2	61	theme	polysaccharides	678:692	arg1	technique					514:522	a novel technique	506:522	a novel technique	506:522	In this study, a novel technique, composite extraction of HPCD each with rhamnolipid (RL), bovine serum albumin (BSA) and alginate sodium (AS), models of lipopolysaccharide, proteins and polysaccharides, was developed to assess PAHs bioaccessibility to earthworms.
27395072	2	61	theme	polysaccharides	678:692	arg1	extraction					535:544	composite extraction	525:544	composite extraction of HPCD each with rhamnolipid (RL), bovine serum albumin (BSA) and alginate sodium (AS), models of lipopolysaccharide, proteins and polysaccharides	525:692	In this study, a novel technique, composite extraction of HPCD each with rhamnolipid (RL), bovine serum albumin (BSA) and alginate sodium (AS), models of lipopolysaccharide, proteins and polysaccharides, was developed to assess PAHs bioaccessibility to earthworms.
27395072	1	62	theme	HPCD	184:187	arg1	extraction					190:199	Hydroxypropyl-β-cyclodextrin (HPCD) extraction	154:199	Hydroxypropyl-β-cyclodextrin (HPCD) extraction	154:199	Hydroxypropyl-β-cyclodextrin (HPCD) extraction has been widely used to estimate bioaccessibility of polycyclic aromatic hydrocarbons (PAHs) in soil, but it often underestimates the actual risk due to lack of information regarding the exogenous active substances, such as extracellular polymer substance (EPS) secreted by microorganisms.
27395072	3	63	theme	individual	842:851	arg1	extraction					858:867	individual HPCD extraction	842:867	individual HPCD extraction	842:867	In addition, comparisons were conducted with accelerated solvent extraction (ASE) and individual HPCD extraction.
28847077	5	0	theme	efficiency	820:829	arg1	terms					797:801	terms	797:801	terms of fractionation efficiency and enzymatic digestibility	797:857	Especially Szarvasi pulp displayed outstanding results in terms of fractionation efficiency and enzymatic digestibility, though in all cases successful lignocellulose fractionation was observed.
28847077	6	1	theme	plant	1004:1008	arg1	species					1010:1016	different perennial plant species	984:1016	different perennial plant species	984:1016	These insights into the structural composition of different perennial plant species and the impact of the OrganoCat pretreatment on the plant material leads to useful information to strategically adapt such processes to the individual lignocellulosic material aiming for a full valorisation.
28847077	1	2	with	pretreatment	161:172	arg1	production					191:200	sustainable production	179:200	sustainable production of biomasses	179:213	A successful biorefinery needs to align suitable pretreatment with sustainable production of biomasses.
28847077	6	3	theme	such	1136:1139	arg1	processes					1141:1149	such processes	1136:1149	such processes	1136:1149	These insights into the structural composition of different perennial plant species and the impact of the OrganoCat pretreatment on the plant material leads to useful information to strategically adapt such processes to the individual lignocellulosic material aiming for a full valorisation.
28847077	4	4	theme	Thorough	539:546	arg1	analyses					548:555	Thorough analyses	539:555	Thorough analyses of the lignocellulose - before and after fractionation -	539:612	Thorough analyses of the lignocellulose - before and after fractionation - enabled a detailed description of the fate of cellulosic, non-cellulosic polysaccharides and lignin during the pretreatment.
28847077	3	5	theme	non-grasses	526:536	arg1	efficient					508:516	efficient	508:516	efficient	508:516	The technology was successfully applied to the different perennial plants revealing that pretreatment of grasses was more efficient than of non-grasses.
28847077	5	6	theme	outstanding	774:784	arg1	results					786:792	outstanding results	774:792	outstanding results	774:792	Especially Szarvasi pulp displayed outstanding results in terms of fractionation efficiency and enzymatic digestibility, though in all cases successful lignocellulose fractionation was observed.
28847077	6	7	theme	different	984:992	arg1	species					1010:1016	different perennial plant species	984:1016	different perennial plant species	984:1016	These insights into the structural composition of different perennial plant species and the impact of the OrganoCat pretreatment on the plant material leads to useful information to strategically adapt such processes to the individual lignocellulosic material aiming for a full valorisation.
28847077	6	8	theme	plant	1070:1074	arg1	material					1076:1083	the plant material	1066:1083	the plant material	1066:1083	These insights into the structural composition of different perennial plant species and the impact of the OrganoCat pretreatment on the plant material leads to useful information to strategically adapt such processes to the individual lignocellulosic material aiming for a full valorisation.
28847077	0	9	theme	valuable	91:98	arg1	feedstocks					100:109	valuable feedstocks	91:109	valuable feedstocks	91:109	OrganoCat pretreatment of perennial plants: Synergies between a biogenic fractionation and valuable feedstocks.
28847077	6	10	theme	full	1207:1210	arg1	valorisation					1212:1223	a full valorisation	1205:1223	a full valorisation	1205:1223	These insights into the structural composition of different perennial plant species and the impact of the OrganoCat pretreatment on the plant material leads to useful information to strategically adapt such processes to the individual lignocellulosic material aiming for a full valorisation.
28847077	4	11	theme	fate	652:655	arg1	description					633:643	a detailed description	622:643	a detailed description of the fate of cellulosic, non-cellulosic polysaccharides	622:701	Thorough analyses of the lignocellulose - before and after fractionation - enabled a detailed description of the fate of cellulosic, non-cellulosic polysaccharides and lignin during the pretreatment.
28847077	4	11	theme	fate	652:655	arg1	lignin					707:712	lignin	707:712	lignin	707:712	Thorough analyses of the lignocellulose - before and after fractionation - enabled a detailed description of the fate of cellulosic, non-cellulosic polysaccharides and lignin during the pretreatment.
28847077	1	12	theme	sustainable	179:189	arg1	production					191:200	sustainable production	179:200	sustainable production of biomasses	179:213	A successful biorefinery needs to align suitable pretreatment with sustainable production of biomasses.
28847077	6	13	theme	structural	958:967	arg1	composition					969:979	the structural composition	954:979	the structural composition of different perennial plant species	954:1016	These insights into the structural composition of different perennial plant species and the impact of the OrganoCat pretreatment on the plant material leads to useful information to strategically adapt such processes to the individual lignocellulosic material aiming for a full valorisation.
28847077	2	14	dep	Sida	248:251	arg1	Szarvasi					279:286	Szarvasi	279:286	Szarvasi	279:286	Herein, four perennial plants, (Sida, Silphium, Miscanthus and Szarvasi) regarded as promising feedstocks for biorefineries were subjected to the OrganoCat pretreatment.
28847077	2	14	dep	Sida	248:251	arg1	Miscanthus					264:273	Miscanthus	264:273	Miscanthus	264:273	Herein, four perennial plants, (Sida, Silphium, Miscanthus and Szarvasi) regarded as promising feedstocks for biorefineries were subjected to the OrganoCat pretreatment.
28847077	2	14	dep	Sida	248:251	arg1	Silphium					254:261	Silphium	254:261	Silphium	254:261	Herein, four perennial plants, (Sida, Silphium, Miscanthus and Szarvasi) regarded as promising feedstocks for biorefineries were subjected to the OrganoCat pretreatment.
28847077	6	15	theme	lignocellulosic	1169:1183	arg1	material					1185:1192	the individual lignocellulosic material	1154:1192	the individual lignocellulosic material aiming for a full valorisation	1154:1223	These insights into the structural composition of different perennial plant species and the impact of the OrganoCat pretreatment on the plant material leads to useful information to strategically adapt such processes to the individual lignocellulosic material aiming for a full valorisation.
28847077	3	16	theme	different	433:441	arg1	plants					453:458	the different perennial plants	429:458	the different perennial plants	429:458	The technology was successfully applied to the different perennial plants revealing that pretreatment of grasses was more efficient than of non-grasses.
28847077	0	17	theme	OrganoCat	0:8	arg1	pretreatment					10:21	OrganoCat pretreatment	0:21	OrganoCat pretreatment of perennial plants: Synergies between a biogenic fractionation and valuable feedstocks.	0:110	OrganoCat pretreatment of perennial plants: Synergies between a biogenic fractionation and valuable feedstocks.
28847077	2	18	theme	promising	301:309	arg1	feedstocks					311:320	promising feedstocks	301:320	promising feedstocks for biorefineries	301:338	Herein, four perennial plants, (Sida, Silphium, Miscanthus and Szarvasi) regarded as promising feedstocks for biorefineries were subjected to the OrganoCat pretreatment.
28847077	3	19	theme	perennial	443:451	arg1	plants					453:458	the different perennial plants	429:458	the different perennial plants	429:458	The technology was successfully applied to the different perennial plants revealing that pretreatment of grasses was more efficient than of non-grasses.
28847077	0	20	theme	perennial	26:34	arg1	plants					36:41	perennial plants	26:41	perennial plants	26:41	OrganoCat pretreatment of perennial plants: Synergies between a biogenic fractionation and valuable feedstocks.
28847077	6	21	theme	pretreatment	1050:1061	arg1	impact					1026:1031	the impact	1022:1031	the impact of the OrganoCat pretreatment on the plant material	1022:1083	These insights into the structural composition of different perennial plant species and the impact of the OrganoCat pretreatment on the plant material leads to useful information to strategically adapt such processes to the individual lignocellulosic material aiming for a full valorisation.
28847077	6	21	theme	pretreatment	1050:1061	arg1	insights					940:947	These insights	934:947	These insights into the structural composition of different perennial plant species	934:1016	These insights into the structural composition of different perennial plant species and the impact of the OrganoCat pretreatment on the plant material leads to useful information to strategically adapt such processes to the individual lignocellulosic material aiming for a full valorisation.
28847077	2	22	dep	plants	239:244	arg1	Sida					248:251	Sida	248:251	Sida	248:251	Herein, four perennial plants, (Sida, Silphium, Miscanthus and Szarvasi) regarded as promising feedstocks for biorefineries were subjected to the OrganoCat pretreatment.
28847077	5	23	theme	successful	880:889	arg1	fractionation					906:918	successful lignocellulose fractionation	880:918	successful lignocellulose fractionation	880:918	Especially Szarvasi pulp displayed outstanding results in terms of fractionation efficiency and enzymatic digestibility, though in all cases successful lignocellulose fractionation was observed.
28847077	6	24	theme	OrganoCat	1040:1048	arg1	pretreatment					1050:1061	the OrganoCat pretreatment	1036:1061	the OrganoCat pretreatment	1036:1061	These insights into the structural composition of different perennial plant species and the impact of the OrganoCat pretreatment on the plant material leads to useful information to strategically adapt such processes to the individual lignocellulosic material aiming for a full valorisation.
28847077	5	25	theme	Szarvasi	750:757	arg1	pulp					759:762	Especially Szarvasi pulp	739:762	Especially Szarvasi pulp	739:762	Especially Szarvasi pulp displayed outstanding results in terms of fractionation efficiency and enzymatic digestibility, though in all cases successful lignocellulose fractionation was observed.
28847077	3	26	theme	grasses	491:497	arg1	pretreatment					475:486	pretreatment	475:486	pretreatment of grasses	475:497	The technology was successfully applied to the different perennial plants revealing that pretreatment of grasses was more efficient than of non-grasses.
28847077	5	27	theme	enzymatic	835:843	arg1	digestibility					845:857	enzymatic digestibility	835:857	enzymatic digestibility	835:857	Especially Szarvasi pulp displayed outstanding results in terms of fractionation efficiency and enzymatic digestibility, though in all cases successful lignocellulose fractionation was observed.
28847077	1	28	theme	biomasses	205:213	arg1	production					191:200	sustainable production	179:200	sustainable production of biomasses	179:213	A successful biorefinery needs to align suitable pretreatment with sustainable production of biomasses.
28847077	4	29	theme	polysaccharides	687:701	arg1	fate					652:655	the fate	648:655	the fate of cellulosic, non-cellulosic polysaccharides	648:701	Thorough analyses of the lignocellulose - before and after fractionation - enabled a detailed description of the fate of cellulosic, non-cellulosic polysaccharides and lignin during the pretreatment.
28847077	0	30	theme	plants	36:41	arg1	pretreatment					10:21	OrganoCat pretreatment	0:21	OrganoCat pretreatment of perennial plants: Synergies between a biogenic fractionation and valuable feedstocks.	0:110	OrganoCat pretreatment of perennial plants: Synergies between a biogenic fractionation and valuable feedstocks.
28847077	4	31	dep	cellulosic	660:669	arg1	non-cellulosic					672:685	non-cellulosic	672:685	non-cellulosic	672:685	Thorough analyses of the lignocellulose - before and after fractionation - enabled a detailed description of the fate of cellulosic, non-cellulosic polysaccharides and lignin during the pretreatment.
28847077	4	32	theme	cellulosic	660:669	arg1	polysaccharides					687:701	cellulosic, non-cellulosic polysaccharides	660:701	cellulosic, non-cellulosic polysaccharides	660:701	Thorough analyses of the lignocellulose - before and after fractionation - enabled a detailed description of the fate of cellulosic, non-cellulosic polysaccharides and lignin during the pretreatment.
28847077	1	33	theme	successful	114:123	arg1	biorefinery					125:135	A successful biorefinery	112:135	A successful biorefinery	112:135	A successful biorefinery needs to align suitable pretreatment with sustainable production of biomasses.
28847077	4	34	theme	lignocellulose	564:577	arg1	analyses					548:555	Thorough analyses	539:555	Thorough analyses of the lignocellulose - before and after fractionation -	539:612	Thorough analyses of the lignocellulose - before and after fractionation - enabled a detailed description of the fate of cellulosic, non-cellulosic polysaccharides and lignin during the pretreatment.
28847077	6	35	theme	perennial	994:1002	arg1	species					1010:1016	different perennial plant species	984:1016	different perennial plant species	984:1016	These insights into the structural composition of different perennial plant species and the impact of the OrganoCat pretreatment on the plant material leads to useful information to strategically adapt such processes to the individual lignocellulosic material aiming for a full valorisation.
28847077	2	36	theme	perennial	229:237	arg1	plants					239:244	four perennial plants	224:244	four perennial plants, (Sida, Silphium, Miscanthus and Szarvasi) regarded as promising feedstocks for biorefineries	224:338	Herein, four perennial plants, (Sida, Silphium, Miscanthus and Szarvasi) regarded as promising feedstocks for biorefineries were subjected to the OrganoCat pretreatment.
28847077	6	37	from	insights	940:947	arg1	material					1076:1083	the plant material	1066:1083	the plant material	1066:1083	These insights into the structural composition of different perennial plant species and the impact of the OrganoCat pretreatment on the plant material leads to useful information to strategically adapt such processes to the individual lignocellulosic material aiming for a full valorisation.
28847077	6	38	from	impact	1026:1031	arg1	material					1076:1083	the plant material	1066:1083	the plant material	1066:1083	These insights into the structural composition of different perennial plant species and the impact of the OrganoCat pretreatment on the plant material leads to useful information to strategically adapt such processes to the individual lignocellulosic material aiming for a full valorisation.
28847077	5	39	located	observed	924:931	arg1	cases					874:878	all cases	870:878	all cases	870:878	Especially Szarvasi pulp displayed outstanding results in terms of fractionation efficiency and enzymatic digestibility, though in all cases successful lignocellulose fractionation was observed.
28847077	5	39	located	observed	924:931	arg2	fractionation					906:918	successful lignocellulose fractionation	880:918	successful lignocellulose fractionation	880:918	Especially Szarvasi pulp displayed outstanding results in terms of fractionation efficiency and enzymatic digestibility, though in all cases successful lignocellulose fractionation was observed.
28847077	1	40	theme	suitable	152:159	arg1	pretreatment					161:172	suitable pretreatment	152:172	suitable pretreatment with sustainable production of biomasses	152:213	A successful biorefinery needs to align suitable pretreatment with sustainable production of biomasses.
28847077	4	41	theme	detailed	624:631	arg1	description					633:643	a detailed description	622:643	a detailed description of the fate of cellulosic, non-cellulosic polysaccharides	622:701	Thorough analyses of the lignocellulose - before and after fractionation - enabled a detailed description of the fate of cellulosic, non-cellulosic polysaccharides and lignin during the pretreatment.
28847077	0	42	theme	biogenic	64:71	arg1	fractionation					73:85	a biogenic fractionation	62:85	a biogenic fractionation	62:85	OrganoCat pretreatment of perennial plants: Synergies between a biogenic fractionation and valuable feedstocks.
28847077	0	43	dep	pretreatment	10:21	arg1	Synergies					44:52	Synergies	44:52	OrganoCat pretreatment of perennial plants: Synergies between a biogenic fractionation and valuable feedstocks.	0:110	OrganoCat pretreatment of perennial plants: Synergies between a biogenic fractionation and valuable feedstocks.
28847077	5	44	theme	lignocellulose	891:904	arg1	fractionation					906:918	successful lignocellulose fractionation	880:918	successful lignocellulose fractionation	880:918	Especially Szarvasi pulp displayed outstanding results in terms of fractionation efficiency and enzymatic digestibility, though in all cases successful lignocellulose fractionation was observed.
28847077	6	45	theme	individual	1158:1167	arg1	material					1185:1192	the individual lignocellulosic material	1154:1192	the individual lignocellulosic material aiming for a full valorisation	1154:1223	These insights into the structural composition of different perennial plant species and the impact of the OrganoCat pretreatment on the plant material leads to useful information to strategically adapt such processes to the individual lignocellulosic material aiming for a full valorisation.
28847077	6	46	theme	useful	1094:1099	arg1	information					1101:1111	useful information	1094:1111	useful information to strategically adapt such processes to the individual lignocellulosic material aiming for a full valorisation	1094:1223	These insights into the structural composition of different perennial plant species and the impact of the OrganoCat pretreatment on the plant material leads to useful information to strategically adapt such processes to the individual lignocellulosic material aiming for a full valorisation.
28847077	5	47	theme	fractionation	806:818	arg1	efficiency					820:829	fractionation efficiency	806:829	fractionation efficiency	806:829	Especially Szarvasi pulp displayed outstanding results in terms of fractionation efficiency and enzymatic digestibility, though in all cases successful lignocellulose fractionation was observed.
28847077	6	48	theme	species	1010:1016	arg1	composition					969:979	the structural composition	954:979	the structural composition of different perennial plant species	954:1016	These insights into the structural composition of different perennial plant species and the impact of the OrganoCat pretreatment on the plant material leads to useful information to strategically adapt such processes to the individual lignocellulosic material aiming for a full valorisation.
28847077	5	49	theme	digestibility	845:857	arg1	terms					797:801	terms	797:801	terms of fractionation efficiency and enzymatic digestibility	797:857	Especially Szarvasi pulp displayed outstanding results in terms of fractionation efficiency and enzymatic digestibility, though in all cases successful lignocellulose fractionation was observed.
28847077	2	50	theme	OrganoCat	362:370	arg1	pretreatment					372:383	the OrganoCat pretreatment	358:383	the OrganoCat pretreatment	358:383	Herein, four perennial plants, (Sida, Silphium, Miscanthus and Szarvasi) regarded as promising feedstocks for biorefineries were subjected to the OrganoCat pretreatment.
28223552	5	0	theme	Ca-	880:882	arg1	microparticles					896:909	Zn-poor, Ca- and Mg-rich microparticles	871:909	Zn-poor, Ca- and Mg-rich microparticles	871:909	Zn-rich microparticles were 'star shaped' and appeared to consist of small crystallites, while Zn-poor, Ca- and Mg-rich microparticles were irregular in shape and appeared to contain lager crystallites.
28223552	7	1	theme	injectable	1300:1309	arg1	biomaterial					1311:1321	an injectable biomaterial	1297:1321	an injectable biomaterial	1297:1321	Composites containing Zn-free microparticles were cytocompatible and therefore appear most suitable for applications as an injectable biomaterial.
28223552	0	2	theme	tissue	146:151	arg1	regeneration					153:164	tissue regeneration	146:164	tissue regeneration	146:164	Ca:Mg:Zn:CO3 and Ca:Mg:CO3-tri- and bi-elemental carbonate microparticles for novel injectable self-gelling hydrogel-microparticle composites for tissue regeneration.
28223552	7	3	contain	containing	1188:1197	arg1	Composites					1177:1186	Composites	1177:1186	Composites containing Zn-free microparticles	1177:1220	Composites containing Zn-free microparticles were cytocompatible and therefore appear most suitable for applications as an injectable biomaterial.
28223552	7	3	contain	containing	1188:1197	arg2	microparticles					1207:1220	Zn-free microparticles	1199:1220	Zn-free microparticles	1199:1220	Composites containing Zn-free microparticles were cytocompatible and therefore appear most suitable for applications as an injectable biomaterial.
28223552	2	4	theme	carbonate	334:342	arg1	microparticles					344:357	multifunctional carbonate microparticles	318:357	multifunctional carbonate microparticles containing different amounts of calcium, magnesium and zinc	318:417	In this study, multifunctional carbonate microparticles containing different amounts of calcium, magnesium and zinc were mixed with solutions of gellan gum (GG), an anionic polysaccharide, to form injectable hydrogel-microparticle composites, containing Zn, Ca and Mg.
28223552	6	5	theme	microparticle	987:999	arg1	groups					1001:1006	Zn-free microparticle groups	979:1006	Zn-free microparticle groups	979:1006	Zn-free microparticle groups exhibited the best cytocompatibility and, unexpectedly, Zn-free composites showed the highest antibacterial activity towards methicilin-resistant Staphylococcus aureus.
28223552	4	6	theme	elemental	753:761	arg1	composition					763:773	elemental composition	753:773	elemental composition	753:773	Microparticle groups were heterogeneous and contained microparticles of differing shape and elemental composition.
28223552	8	7	theme	hydrogel-microparticle	1455:1476	arg1	composites					1478:1487	injectable hydrogel-microparticle composites	1444:1487	injectable hydrogel-microparticle composites	1444:1487	This study proves the principle of creating bi- and tri-elemental microparticles to induce the gelation of GG to create injectable hydrogel-microparticle composites.
28223552	1	8	theme	inorganic	247:255	arg1	microparticles					257:270	inorganic microparticles	247:270	inorganic microparticles	247:270	Injectable composites for tissue regeneration can be developed by dispersion of inorganic microparticles and cells in a hydrogel phase.
28223552	0	9	theme	CO3-tri-	23:30	arg1	microparticles					59:72	CO3-tri- and bi-elemental carbonate microparticles	23:72	Ca:Mg:Zn:CO3 and Ca:Mg:CO3-tri- and bi-elemental carbonate microparticles for novel injectable self-gelling hydrogel-microparticle composites for tissue regeneration.	0:165	Ca:Mg:Zn:CO3 and Ca:Mg:CO3-tri- and bi-elemental carbonate microparticles for novel injectable self-gelling hydrogel-microparticle composites for tissue regeneration.
28223552	6	10	theme	antibacterial	1102:1114	arg1	activity					1116:1123	the highest antibacterial activity	1090:1123	the highest antibacterial activity towards methicilin-resistant Staphylococcus aureus	1090:1174	Zn-free microparticle groups exhibited the best cytocompatibility and, unexpectedly, Zn-free composites showed the highest antibacterial activity towards methicilin-resistant Staphylococcus aureus.
28223552	1	11	theme	microparticles	257:270	arg1	dispersion					233:242	dispersion	233:242	dispersion of inorganic microparticles and cells in a hydrogel phase	233:300	Injectable composites for tissue regeneration can be developed by dispersion of inorganic microparticles and cells in a hydrogel phase.
28223552	0	12	theme	carbonate	49:57	arg1	microparticles					59:72	CO3-tri- and bi-elemental carbonate microparticles	23:72	Ca:Mg:Zn:CO3 and Ca:Mg:CO3-tri- and bi-elemental carbonate microparticles for novel injectable self-gelling hydrogel-microparticle composites for tissue regeneration.	0:165	Ca:Mg:Zn:CO3 and Ca:Mg:CO3-tri- and bi-elemental carbonate microparticles for novel injectable self-gelling hydrogel-microparticle composites for tissue regeneration.
28223552	4	13	contain	contained	705:713	arg2	microparticles					715:728	microparticles	715:728	microparticles of differing shape and elemental composition	715:773	Microparticle groups were heterogeneous and contained microparticles of differing shape and elemental composition.
28223552	4	13	contain	contained	705:713	arg1	groups					675:680	Microparticle groups	661:680	Microparticle groups	661:680	Microparticle groups were heterogeneous and contained microparticles of differing shape and elemental composition.
28223552	5	14	theme	lager	959:963	arg1	crystallites					965:976	lager crystallites	959:976	lager crystallites	959:976	Zn-rich microparticles were 'star shaped' and appeared to consist of small crystallites, while Zn-poor, Ca- and Mg-rich microparticles were irregular in shape and appeared to contain lager crystallites.
28223552	5	15	theme	small	845:849	arg1	crystallites					851:862	small crystallites	845:862	small crystallites	845:862	Zn-rich microparticles were 'star shaped' and appeared to consist of small crystallites, while Zn-poor, Ca- and Mg-rich microparticles were irregular in shape and appeared to contain lager crystallites.
28223552	0	16	theme	bi-elemental	36:47	arg1	microparticles					59:72	CO3-tri- and bi-elemental carbonate microparticles	23:72	Ca:Mg:Zn:CO3 and Ca:Mg:CO3-tri- and bi-elemental carbonate microparticles for novel injectable self-gelling hydrogel-microparticle composites for tissue regeneration.	0:165	Ca:Mg:Zn:CO3 and Ca:Mg:CO3-tri- and bi-elemental carbonate microparticles for novel injectable self-gelling hydrogel-microparticle composites for tissue regeneration.
28223552	2	17	contain	containing	359:368	arg2	calcium					391:397	calcium	391:397	calcium	391:397	In this study, multifunctional carbonate microparticles containing different amounts of calcium, magnesium and zinc were mixed with solutions of gellan gum (GG), an anionic polysaccharide, to form injectable hydrogel-microparticle composites, containing Zn, Ca and Mg.
28223552	2	17	contain	containing	359:368	arg1	microparticles					344:357	multifunctional carbonate microparticles	318:357	multifunctional carbonate microparticles containing different amounts of calcium, magnesium and zinc	318:417	In this study, multifunctional carbonate microparticles containing different amounts of calcium, magnesium and zinc were mixed with solutions of gellan gum (GG), an anionic polysaccharide, to form injectable hydrogel-microparticle composites, containing Zn, Ca and Mg.
28223552	2	17	contain	containing	359:368	arg2	zinc					414:417	zinc	414:417	zinc	414:417	In this study, multifunctional carbonate microparticles containing different amounts of calcium, magnesium and zinc were mixed with solutions of gellan gum (GG), an anionic polysaccharide, to form injectable hydrogel-microparticle composites, containing Zn, Ca and Mg.
28223552	2	17	contain	containing	359:368	arg2	magnesium					400:408	magnesium	400:408	magnesium	400:408	In this study, multifunctional carbonate microparticles containing different amounts of calcium, magnesium and zinc were mixed with solutions of gellan gum (GG), an anionic polysaccharide, to form injectable hydrogel-microparticle composites, containing Zn, Ca and Mg.
28223552	2	17	contain	containing	359:368	arg2	amounts					380:386	different amounts	370:386	different amounts of calcium, magnesium and zinc	370:417	In this study, multifunctional carbonate microparticles containing different amounts of calcium, magnesium and zinc were mixed with solutions of gellan gum (GG), an anionic polysaccharide, to form injectable hydrogel-microparticle composites, containing Zn, Ca and Mg.
28223552	7	18	theme	Zn-free	1199:1205	arg1	microparticles					1207:1220	Zn-free microparticles	1199:1220	Zn-free microparticles	1199:1220	Composites containing Zn-free microparticles were cytocompatible and therefore appear most suitable for applications as an injectable biomaterial.
28223552	8	19	theme	GG	1431:1432	arg1	gelation					1419:1426	the gelation	1415:1426	the gelation of GG to create injectable hydrogel-microparticle composites	1415:1487	This study proves the principle of creating bi- and tri-elemental microparticles to induce the gelation of GG to create injectable hydrogel-microparticle composites.
28223552	5	20	contain	contain	951:957	arg2	crystallites					965:976	lager crystallites	959:976	lager crystallites	959:976	Zn-rich microparticles were 'star shaped' and appeared to consist of small crystallites, while Zn-poor, Ca- and Mg-rich microparticles were irregular in shape and appeared to contain lager crystallites.
28223552	5	20	contain	contain	951:957	arg1	microparticles					896:909	Zn-poor, Ca- and Mg-rich microparticles	871:909	Zn-poor, Ca- and Mg-rich microparticles	871:909	Zn-rich microparticles were 'star shaped' and appeared to consist of small crystallites, while Zn-poor, Ca- and Mg-rich microparticles were irregular in shape and appeared to contain lager crystallites.
28223552	2	21	theme	calcium	391:397	arg1	calcium					391:397	calcium	391:397	calcium	391:397	In this study, multifunctional carbonate microparticles containing different amounts of calcium, magnesium and zinc were mixed with solutions of gellan gum (GG), an anionic polysaccharide, to form injectable hydrogel-microparticle composites, containing Zn, Ca and Mg.
28223552	2	21	theme	calcium	391:397	arg1	amounts					380:386	different amounts	370:386	different amounts of calcium, magnesium and zinc	370:417	In this study, multifunctional carbonate microparticles containing different amounts of calcium, magnesium and zinc were mixed with solutions of gellan gum (GG), an anionic polysaccharide, to form injectable hydrogel-microparticle composites, containing Zn, Ca and Mg.
28223552	2	21	theme	calcium	391:397	arg1	zinc					414:417	zinc	414:417	zinc	414:417	In this study, multifunctional carbonate microparticles containing different amounts of calcium, magnesium and zinc were mixed with solutions of gellan gum (GG), an anionic polysaccharide, to form injectable hydrogel-microparticle composites, containing Zn, Ca and Mg.
28223552	2	21	theme	calcium	391:397	arg1	magnesium					400:408	magnesium	400:408	magnesium	400:408	In this study, multifunctional carbonate microparticles containing different amounts of calcium, magnesium and zinc were mixed with solutions of gellan gum (GG), an anionic polysaccharide, to form injectable hydrogel-microparticle composites, containing Zn, Ca and Mg.
28223552	5	22	from	irregular	916:924	arg1	shape					929:933	shape	929:933	shape	929:933	Zn-rich microparticles were 'star shaped' and appeared to consist of small crystallites, while Zn-poor, Ca- and Mg-rich microparticles were irregular in shape and appeared to contain lager crystallites.
28223552	8	23	theme	injectable	1444:1453	arg1	composites					1478:1487	injectable hydrogel-microparticle composites	1444:1487	injectable hydrogel-microparticle composites	1444:1487	This study proves the principle of creating bi- and tri-elemental microparticles to induce the gelation of GG to create injectable hydrogel-microparticle composites.
28223552	6	24	theme	highest	1094:1100	arg1	activity					1116:1123	the highest antibacterial activity	1090:1123	the highest antibacterial activity towards methicilin-resistant Staphylococcus aureus	1090:1174	Zn-free microparticle groups exhibited the best cytocompatibility and, unexpectedly, Zn-free composites showed the highest antibacterial activity towards methicilin-resistant Staphylococcus aureus.
28223552	1	25	from	dispersion	233:242	arg1	phase					296:300	a hydrogel phase	285:300	a hydrogel phase	285:300	Injectable composites for tissue regeneration can be developed by dispersion of inorganic microparticles and cells in a hydrogel phase.
28223552	2	26	contain	containing	546:555	arg1	solutions					435:443	solutions	435:443	solutions	435:443	In this study, multifunctional carbonate microparticles containing different amounts of calcium, magnesium and zinc were mixed with solutions of gellan gum (GG), an anionic polysaccharide, to form injectable hydrogel-microparticle composites, containing Zn, Ca and Mg.
28223552	2	26	contain	containing	546:555	arg2	Ca					561:562	Ca	561:562	Ca	561:562	In this study, multifunctional carbonate microparticles containing different amounts of calcium, magnesium and zinc were mixed with solutions of gellan gum (GG), an anionic polysaccharide, to form injectable hydrogel-microparticle composites, containing Zn, Ca and Mg.
28223552	2	26	contain	containing	546:555	arg2	Zn					557:558	Zn	557:558	Zn	557:558	In this study, multifunctional carbonate microparticles containing different amounts of calcium, magnesium and zinc were mixed with solutions of gellan gum (GG), an anionic polysaccharide, to form injectable hydrogel-microparticle composites, containing Zn, Ca and Mg.
28223552	2	26	contain	containing	546:555	arg2	Mg					568:569	Mg	568:569	Mg	568:569	In this study, multifunctional carbonate microparticles containing different amounts of calcium, magnesium and zinc were mixed with solutions of gellan gum (GG), an anionic polysaccharide, to form injectable hydrogel-microparticle composites, containing Zn, Ca and Mg.
28223552	1	27	theme	Injectable	167:176	arg1	composites					178:187	Injectable composites	167:187	Injectable composites for tissue regeneration	167:211	Injectable composites for tissue regeneration can be developed by dispersion of inorganic microparticles and cells in a hydrogel phase.
28223552	2	28	theme	hydrogel-microparticle	511:532	arg1	composites					534:543	injectable hydrogel-microparticle composites	500:543	injectable hydrogel-microparticle composites	500:543	In this study, multifunctional carbonate microparticles containing different amounts of calcium, magnesium and zinc were mixed with solutions of gellan gum (GG), an anionic polysaccharide, to form injectable hydrogel-microparticle composites, containing Zn, Ca and Mg.
28223552	1	29	theme	cells	276:280	arg1	dispersion					233:242	dispersion	233:242	dispersion of inorganic microparticles and cells in a hydrogel phase	233:300	Injectable composites for tissue regeneration can be developed by dispersion of inorganic microparticles and cells in a hydrogel phase.
28223552	2	30	dep	polysaccharide	476:489	arg1	form					495:498	form	495:498	to form injectable hydrogel-microparticle composites	492:543	In this study, multifunctional carbonate microparticles containing different amounts of calcium, magnesium and zinc were mixed with solutions of gellan gum (GG), an anionic polysaccharide, to form injectable hydrogel-microparticle composites, containing Zn, Ca and Mg.
28223552	4	31	theme	Microparticle	661:673	arg1	groups					675:680	Microparticle groups	661:680	Microparticle groups	661:680	Microparticle groups were heterogeneous and contained microparticles of differing shape and elemental composition.
28223552	2	32	theme	zinc	414:417	arg1	calcium					391:397	calcium	391:397	calcium	391:397	In this study, multifunctional carbonate microparticles containing different amounts of calcium, magnesium and zinc were mixed with solutions of gellan gum (GG), an anionic polysaccharide, to form injectable hydrogel-microparticle composites, containing Zn, Ca and Mg.
28223552	2	32	theme	zinc	414:417	arg1	amounts					380:386	different amounts	370:386	different amounts of calcium, magnesium and zinc	370:417	In this study, multifunctional carbonate microparticles containing different amounts of calcium, magnesium and zinc were mixed with solutions of gellan gum (GG), an anionic polysaccharide, to form injectable hydrogel-microparticle composites, containing Zn, Ca and Mg.
28223552	2	32	theme	zinc	414:417	arg1	zinc					414:417	zinc	414:417	zinc	414:417	In this study, multifunctional carbonate microparticles containing different amounts of calcium, magnesium and zinc were mixed with solutions of gellan gum (GG), an anionic polysaccharide, to form injectable hydrogel-microparticle composites, containing Zn, Ca and Mg.
28223552	2	32	theme	zinc	414:417	arg1	magnesium					400:408	magnesium	400:408	magnesium	400:408	In this study, multifunctional carbonate microparticles containing different amounts of calcium, magnesium and zinc were mixed with solutions of gellan gum (GG), an anionic polysaccharide, to form injectable hydrogel-microparticle composites, containing Zn, Ca and Mg.
28223552	5	33	theme	Zn-rich	776:782	arg1	microparticles					784:797	Zn-rich microparticles	776:797	Zn-rich microparticles	776:797	Zn-rich microparticles were 'star shaped' and appeared to consist of small crystallites, while Zn-poor, Ca- and Mg-rich microparticles were irregular in shape and appeared to contain lager crystallites.
28223552	0	34	theme	injectable	84:93	arg1	composites					131:140	novel injectable self-gelling hydrogel-microparticle composites	78:140	novel injectable self-gelling hydrogel-microparticle composites	78:140	Ca:Mg:Zn:CO3 and Ca:Mg:CO3-tri- and bi-elemental carbonate microparticles for novel injectable self-gelling hydrogel-microparticle composites for tissue regeneration.
28223552	6	35	theme	Zn-free	1064:1070	arg1	composites					1072:1081	Zn-free composites	1064:1081	Zn-free composites	1064:1081	Zn-free microparticle groups exhibited the best cytocompatibility and, unexpectedly, Zn-free composites showed the highest antibacterial activity towards methicilin-resistant Staphylococcus aureus.
28223552	1	36	theme	tissue	193:198	arg1	regeneration					200:211	tissue regeneration	193:211	tissue regeneration	193:211	Injectable composites for tissue regeneration can be developed by dispersion of inorganic microparticles and cells in a hydrogel phase.
28223552	1	37	theme	hydrogel	287:294	arg1	phase					296:300	a hydrogel phase	285:300	a hydrogel phase	285:300	Injectable composites for tissue regeneration can be developed by dispersion of inorganic microparticles and cells in a hydrogel phase.
28223552	0	38	theme	novel	78:82	arg1	composites					131:140	novel injectable self-gelling hydrogel-microparticle composites	78:140	novel injectable self-gelling hydrogel-microparticle composites	78:140	Ca:Mg:Zn:CO3 and Ca:Mg:CO3-tri- and bi-elemental carbonate microparticles for novel injectable self-gelling hydrogel-microparticle composites for tissue regeneration.
28223552	2	39	theme	injectable	500:509	arg1	composites					534:543	injectable hydrogel-microparticle composites	500:543	injectable hydrogel-microparticle composites	500:543	In this study, multifunctional carbonate microparticles containing different amounts of calcium, magnesium and zinc were mixed with solutions of gellan gum (GG), an anionic polysaccharide, to form injectable hydrogel-microparticle composites, containing Zn, Ca and Mg.
28223552	2	40	theme	magnesium	400:408	arg1	calcium					391:397	calcium	391:397	calcium	391:397	In this study, multifunctional carbonate microparticles containing different amounts of calcium, magnesium and zinc were mixed with solutions of gellan gum (GG), an anionic polysaccharide, to form injectable hydrogel-microparticle composites, containing Zn, Ca and Mg.
28223552	2	40	theme	magnesium	400:408	arg1	amounts					380:386	different amounts	370:386	different amounts of calcium, magnesium and zinc	370:417	In this study, multifunctional carbonate microparticles containing different amounts of calcium, magnesium and zinc were mixed with solutions of gellan gum (GG), an anionic polysaccharide, to form injectable hydrogel-microparticle composites, containing Zn, Ca and Mg.
28223552	2	40	theme	magnesium	400:408	arg1	zinc					414:417	zinc	414:417	zinc	414:417	In this study, multifunctional carbonate microparticles containing different amounts of calcium, magnesium and zinc were mixed with solutions of gellan gum (GG), an anionic polysaccharide, to form injectable hydrogel-microparticle composites, containing Zn, Ca and Mg.
28223552	2	40	theme	magnesium	400:408	arg1	magnesium					400:408	magnesium	400:408	magnesium	400:408	In this study, multifunctional carbonate microparticles containing different amounts of calcium, magnesium and zinc were mixed with solutions of gellan gum (GG), an anionic polysaccharide, to form injectable hydrogel-microparticle composites, containing Zn, Ca and Mg.
28223552	2	41	theme	gum	455:457	arg1	solutions					435:443	solutions	435:443	solutions	435:443	In this study, multifunctional carbonate microparticles containing different amounts of calcium, magnesium and zinc were mixed with solutions of gellan gum (GG), an anionic polysaccharide, to form injectable hydrogel-microparticle composites, containing Zn, Ca and Mg.
28223552	5	42	theme	Zn-poor	871:877	arg1	microparticles					896:909	Zn-poor, Ca- and Mg-rich microparticles	871:909	Zn-poor, Ca- and Mg-rich microparticles	871:909	Zn-rich microparticles were 'star shaped' and appeared to consist of small crystallites, while Zn-poor, Ca- and Mg-rich microparticles were irregular in shape and appeared to contain lager crystallites.
28223552	0	43	theme	hydrogel-microparticle	108:129	arg1	composites					131:140	novel injectable self-gelling hydrogel-microparticle composites	78:140	novel injectable self-gelling hydrogel-microparticle composites	78:140	Ca:Mg:Zn:CO3 and Ca:Mg:CO3-tri- and bi-elemental carbonate microparticles for novel injectable self-gelling hydrogel-microparticle composites for tissue regeneration.
28223552	3	44	theme	microparticle	605:617	arg1	preparations					619:630	microparticle preparations	605:630	microparticle preparations	605:630	Zn and Ca were incorporated into microparticle preparations to a greater extent than Mg.
28223552	2	45	theme	multifunctional	318:332	arg1	microparticles					344:357	multifunctional carbonate microparticles	318:357	multifunctional carbonate microparticles containing different amounts of calcium, magnesium and zinc	318:417	In this study, multifunctional carbonate microparticles containing different amounts of calcium, magnesium and zinc were mixed with solutions of gellan gum (GG), an anionic polysaccharide, to form injectable hydrogel-microparticle composites, containing Zn, Ca and Mg.
28223552	2	46	theme	gellan	448:453	arg1	gum					455:457	gellan gum	448:457	gellan gum (GG)	448:462	In this study, multifunctional carbonate microparticles containing different amounts of calcium, magnesium and zinc were mixed with solutions of gellan gum (GG), an anionic polysaccharide, to form injectable hydrogel-microparticle composites, containing Zn, Ca and Mg.
28223552	2	46	theme	gellan	448:453	arg1	polysaccharide					476:489	an anionic polysaccharide	465:489	an anionic polysaccharide	465:489	In this study, multifunctional carbonate microparticles containing different amounts of calcium, magnesium and zinc were mixed with solutions of gellan gum (GG), an anionic polysaccharide, to form injectable hydrogel-microparticle composites, containing Zn, Ca and Mg.
28223552	2	46	theme	gellan	448:453	arg1	GG					460:461	GG	460:461	GG	460:461	In this study, multifunctional carbonate microparticles containing different amounts of calcium, magnesium and zinc were mixed with solutions of gellan gum (GG), an anionic polysaccharide, to form injectable hydrogel-microparticle composites, containing Zn, Ca and Mg.
28223552	0	47	theme	self-gelling	95:106	arg1	composites					131:140	novel injectable self-gelling hydrogel-microparticle composites	78:140	novel injectable self-gelling hydrogel-microparticle composites	78:140	Ca:Mg:Zn:CO3 and Ca:Mg:CO3-tri- and bi-elemental carbonate microparticles for novel injectable self-gelling hydrogel-microparticle composites for tissue regeneration.
28223552	8	48	theme	tri-elemental	1376:1388	arg1	microparticles					1390:1403	tri-elemental microparticles	1376:1403	tri-elemental microparticles	1376:1403	This study proves the principle of creating bi- and tri-elemental microparticles to induce the gelation of GG to create injectable hydrogel-microparticle composites.
28223552	5	49	theme	Mg-rich	888:894	arg1	microparticles					896:909	Zn-poor, Ca- and Mg-rich microparticles	871:909	Zn-poor, Ca- and Mg-rich microparticles	871:909	Zn-rich microparticles were 'star shaped' and appeared to consist of small crystallites, while Zn-poor, Ca- and Mg-rich microparticles were irregular in shape and appeared to contain lager crystallites.
28223552	6	50	theme	best	1022:1025	arg1	cytocompatibility					1027:1043	the best cytocompatibility	1018:1043	the best cytocompatibility	1018:1043	Zn-free microparticle groups exhibited the best cytocompatibility and, unexpectedly, Zn-free composites showed the highest antibacterial activity towards methicilin-resistant Staphylococcus aureus.
28223552	2	51	theme	anionic	468:474	arg1	gum					455:457	gellan gum	448:457	gellan gum (GG)	448:462	In this study, multifunctional carbonate microparticles containing different amounts of calcium, magnesium and zinc were mixed with solutions of gellan gum (GG), an anionic polysaccharide, to form injectable hydrogel-microparticle composites, containing Zn, Ca and Mg.
28223552	2	51	theme	anionic	468:474	arg1	polysaccharide					476:489	an anionic polysaccharide	465:489	an anionic polysaccharide	465:489	In this study, multifunctional carbonate microparticles containing different amounts of calcium, magnesium and zinc were mixed with solutions of gellan gum (GG), an anionic polysaccharide, to form injectable hydrogel-microparticle composites, containing Zn, Ca and Mg.
28223552	3	52	theme	greater	637:643	arg1	extent					645:650	a greater extent	635:650	a greater extent than Mg	635:658	Zn and Ca were incorporated into microparticle preparations to a greater extent than Mg.
28223552	2	53	theme	different	370:378	arg1	calcium					391:397	calcium	391:397	calcium	391:397	In this study, multifunctional carbonate microparticles containing different amounts of calcium, magnesium and zinc were mixed with solutions of gellan gum (GG), an anionic polysaccharide, to form injectable hydrogel-microparticle composites, containing Zn, Ca and Mg.
28223552	2	53	theme	different	370:378	arg1	amounts					380:386	different amounts	370:386	different amounts of calcium, magnesium and zinc	370:417	In this study, multifunctional carbonate microparticles containing different amounts of calcium, magnesium and zinc were mixed with solutions of gellan gum (GG), an anionic polysaccharide, to form injectable hydrogel-microparticle composites, containing Zn, Ca and Mg.
28223552	2	53	theme	different	370:378	arg1	zinc					414:417	zinc	414:417	zinc	414:417	In this study, multifunctional carbonate microparticles containing different amounts of calcium, magnesium and zinc were mixed with solutions of gellan gum (GG), an anionic polysaccharide, to form injectable hydrogel-microparticle composites, containing Zn, Ca and Mg.
28223552	2	53	theme	different	370:378	arg1	magnesium					400:408	magnesium	400:408	magnesium	400:408	In this study, multifunctional carbonate microparticles containing different amounts of calcium, magnesium and zinc were mixed with solutions of gellan gum (GG), an anionic polysaccharide, to form injectable hydrogel-microparticle composites, containing Zn, Ca and Mg.
28223552	5	54	from	shape	929:933	arg1	irregular					916:924	irregular	916:924	irregular	916:924	Zn-rich microparticles were 'star shaped' and appeared to consist of small crystallites, while Zn-poor, Ca- and Mg-rich microparticles were irregular in shape and appeared to contain lager crystallites.
28223552	6	55	theme	Zn-free	979:985	arg1	groups					1001:1006	Zn-free microparticle groups	979:1006	Zn-free microparticle groups	979:1006	Zn-free microparticle groups exhibited the best cytocompatibility and, unexpectedly, Zn-free composites showed the highest antibacterial activity towards methicilin-resistant Staphylococcus aureus.
28223552	0	56	dep	Ca	0:1	arg1	Mg					3:4	Mg	3:4	Ca:Mg:Zn:CO3 and Ca:Mg:CO3-tri- and bi-elemental carbonate microparticles for novel injectable self-gelling hydrogel-microparticle composites for tissue regeneration.	0:165	Ca:Mg:Zn:CO3 and Ca:Mg:CO3-tri- and bi-elemental carbonate microparticles for novel injectable self-gelling hydrogel-microparticle composites for tissue regeneration.
28223552	0	56	dep	Ca	0:1	arg1	Ca					17:18	Ca	17:18	Ca	17:18	Ca:Mg:Zn:CO3 and Ca:Mg:CO3-tri- and bi-elemental carbonate microparticles for novel injectable self-gelling hydrogel-microparticle composites for tissue regeneration.
28223552	0	56	dep	Ca	0:1	arg1	Mg					20:21	Mg	20:21	Ca:Mg:Zn:CO3 and Ca:Mg:CO3-tri- and bi-elemental carbonate microparticles for novel injectable self-gelling hydrogel-microparticle composites for tissue regeneration.	0:165	Ca:Mg:Zn:CO3 and Ca:Mg:CO3-tri- and bi-elemental carbonate microparticles for novel injectable self-gelling hydrogel-microparticle composites for tissue regeneration.
28223552	0	56	dep	Ca	0:1	arg1	microparticles					59:72	CO3-tri- and bi-elemental carbonate microparticles	23:72	Ca:Mg:Zn:CO3 and Ca:Mg:CO3-tri- and bi-elemental carbonate microparticles for novel injectable self-gelling hydrogel-microparticle composites for tissue regeneration.	0:165	Ca:Mg:Zn:CO3 and Ca:Mg:CO3-tri- and bi-elemental carbonate microparticles for novel injectable self-gelling hydrogel-microparticle composites for tissue regeneration.
28223552	0	56	dep	Ca	0:1	arg1	Zn					6:7	Zn	6:7	Ca:Mg:Zn:CO3 and Ca:Mg:CO3-tri- and bi-elemental carbonate microparticles for novel injectable self-gelling hydrogel-microparticle composites for tissue regeneration.	0:165	Ca:Mg:Zn:CO3 and Ca:Mg:CO3-tri- and bi-elemental carbonate microparticles for novel injectable self-gelling hydrogel-microparticle composites for tissue regeneration.
28223552	0	56	dep	Ca	0:1	arg1	CO3					9:11	CO3	9:11	CO3	9:11	Ca:Mg:Zn:CO3 and Ca:Mg:CO3-tri- and bi-elemental carbonate microparticles for novel injectable self-gelling hydrogel-microparticle composites for tissue regeneration.
25618319	4	0	theme	environmental	604:616	arg1	factors					618:624	the optimized environmental factors	590:624	the optimized environmental factors in spray drying of cultured bifidobacteria	590:667	The aim of this study was to establish the optimized environmental factors in spray drying of cultured bifidobacteria to obtain a viable and stable powder.
25618319	6	1	theme	combined	842:849	arg1	effect					851:856	The combined effect	838:856	The combined effect of these variables on survival rateand moisture content of bacterial powder	838:932	The combined effect of these variables on survival rateand moisture content of bacterial powder was studied using a central composite design (CCD).
25618319	2	2	theme	viable	385:390	arg1	microorganisms					392:405	viable microorganisms	385:405	viable microorganisms	385:405	Compared to the traditional lyophilization, spray-drying is an economical process for preparing large quantities of viable microorganisms.
25618319	3	3	theme	high	533:536	arg1	temperature					538:548	high temperature	533:548	high temperature	533:548	The technique offers high production rates and low operating costs but is not usually used for drying of substances prone to high temperature.
25618319	6	4	theme	bacterial	917:925	arg1	powder					927:932	bacterial powder	917:932	bacterial powder	917:932	The combined effect of these variables on survival rateand moisture content of bacterial powder was studied using a central composite design (CCD).
25618319	8	5	theme	different	1257:1265	arg1	amount					1267:1272	different amount	1257:1272	different amount of maltodextrin	1257:1288	The resistant B. bifidum was supplemented with cost friendly permeate, sucrose, yeast extract and different amount of maltodextrin before it was fed into a Buchi B-191 mini spray-dryer.
25618319	8	5	theme	different	1257:1265	arg1	maltodextrin					1277:1288	maltodextrin	1277:1288	maltodextrin	1277:1288	The resistant B. bifidum was supplemented with cost friendly permeate, sucrose, yeast extract and different amount of maltodextrin before it was fed into a Buchi B-191 mini spray-dryer.
25618319	11	6	theme	powder	1704:1709	arg1	content					1682:1688	minimum moisture content	1665:1688	minimum moisture content	1665:1688	The optimal values of the variables for maximum survival and minimum moisture content of B. bifidum powder were as follows: inlet air temperature of 111.15°C, air pressure of 4.5 bar and maltodextrin concentration of 6%.
25618319	11	6	theme	powder	1704:1709	arg1	survival					1652:1659	maximum survival	1644:1659	maximum survival	1644:1659	The optimal values of the variables for maximum survival and minimum moisture content of B. bifidum powder were as follows: inlet air temperature of 111.15°C, air pressure of 4.5 bar and maltodextrin concentration of 6%.
25618319	7	7	theme	outlet	1119:1124	arg1	temperature					1126:1136	high outlet temperature	1114:1136	high outlet temperature during spray drying	1114:1156	Sub-lethal heat-adaptation of a B. bifidum strain which was previously adapted to acid-bile-NaCl led to much more resistance to high outlet temperature during spray drying.
25618319	13	8	theme	Viable	1946:1951	arg1	drying					1978:1983	CONCLUSION Viable and cost effective spray drying	1935:1983	CONCLUSION Viable and cost effective spray drying of Bifidobacterium bifidum	1935:2010	CONCLUSION Viable and cost effective spray drying of Bifidobacterium bifidum could be achieved by cultivating heat and acid adapted strain into the culture media containing nutritional protective agents.
25618319	13	9	theme	spray	1972:1976	arg1	drying					1978:1983	CONCLUSION Viable and cost effective spray drying	1935:1983	CONCLUSION Viable and cost effective spray drying of Bifidobacterium bifidum	1935:2010	CONCLUSION Viable and cost effective spray drying of Bifidobacterium bifidum could be achieved by cultivating heat and acid adapted strain into the culture media containing nutritional protective agents.
25618319	7	10	theme	more	1095:1098	arg1	resistance					1100:1109	much more resistance	1090:1109	much more resistance to high outlet temperature during spray drying	1090:1156	Sub-lethal heat-adaptation of a B. bifidum strain which was previously adapted to acid-bile-NaCl led to much more resistance to high outlet temperature during spray drying.
25618319	1	11	theme	immune	216:221	arg1	system					223:228	the immune system	212:228	the immune system	212:228	BACKGROUND There is substantial clinical data supporting the role of Bifidobacterium bifidum in human health particularly in benefiting the immune system and suppressing intestinal infections.
25618319	6	12	from	effect	851:856	arg1	content					906:912	survival rateand moisture content	880:912	survival rateand moisture content of bacterial powder	880:932	The combined effect of these variables on survival rateand moisture content of bacterial powder was studied using a central composite design (CCD).
25618319	1	13	dep	BACKGROUND	76:85	arg1	is					93:94	is	93:94	is substantial clinical data supporting the role of Bifidobacterium bifidum in human health particularly in benefiting the immune system and suppressing intestinal infections	93:266	BACKGROUND There is substantial clinical data supporting the role of Bifidobacterium bifidum in human health particularly in benefiting the immune system and suppressing intestinal infections.
25618319	13	14	theme	nutritional	2108:2118	arg1	agents					2131:2136	nutritional protective agents	2108:2136	nutritional protective agents	2108:2136	CONCLUSION Viable and cost effective spray drying of Bifidobacterium bifidum could be achieved by cultivating heat and acid adapted strain into the culture media containing nutritional protective agents.
25618319	13	15	contain	containing	2097:2106	arg1	media					2091:2095	the culture media	2079:2095	the culture media containing nutritional protective agents	2079:2136	CONCLUSION Viable and cost effective spray drying of Bifidobacterium bifidum could be achieved by cultivating heat and acid adapted strain into the culture media containing nutritional protective agents.
25618319	13	15	contain	containing	2097:2106	arg2	agents					2131:2136	nutritional protective agents	2108:2136	nutritional protective agents	2108:2136	CONCLUSION Viable and cost effective spray drying of Bifidobacterium bifidum could be achieved by cultivating heat and acid adapted strain into the culture media containing nutritional protective agents.
25618319	6	16	theme	central	954:960	arg1	design					972:977	a central composite design	952:977	a central composite design (CCD)	952:983	The combined effect of these variables on survival rateand moisture content of bacterial powder was studied using a central composite design (CCD).
25618319	6	16	theme	central	954:960	arg1	CCD					980:982	CCD	980:982	CCD	980:982	The combined effect of these variables on survival rateand moisture content of bacterial powder was studied using a central composite design (CCD).
25618319	8	17	theme	Buchi	1315:1319	arg1	spray-dryer					1332:1342	a Buchi B-191 mini spray-dryer	1313:1342	a Buchi B-191 mini spray-dryer	1313:1342	The resistant B. bifidum was supplemented with cost friendly permeate, sucrose, yeast extract and different amount of maltodextrin before it was fed into a Buchi B-191 mini spray-dryer.
25618319	8	18	theme	mini	1327:1330	arg1	spray-dryer					1332:1342	a Buchi B-191 mini spray-dryer	1313:1342	a Buchi B-191 mini spray-dryer	1313:1342	The resistant B. bifidum was supplemented with cost friendly permeate, sucrose, yeast extract and different amount of maltodextrin before it was fed into a Buchi B-191 mini spray-dryer.
25618319	1	19	theme	bifidum	161:167	arg1	role					137:140	the role	133:140	the role of Bifidobacterium bifidum in human health	133:183	BACKGROUND There is substantial clinical data supporting the role of Bifidobacterium bifidum in human health particularly in benefiting the immune system and suppressing intestinal infections.
25618319	10	20	theme	experiments	1494:1504	arg1	Results					1470:1476	Results	1470:1476	Results of verification experiments	1470:1504	Results of verification experiments and predicted values from fitted correlations were in close agreement at 95% confidence interval.
25618319	10	20	theme	experiments	1494:1504	arg1	values					1520:1525	predicted values	1510:1525	predicted values from fitted correlations	1510:1550	Results of verification experiments and predicted values from fitted correlations were in close agreement at 95% confidence interval.
25618319	5	21	theme	air	794:796	arg1	pressure					798:805	air pressure	794:805	air pressure	794:805	METHODS The experiments were designed to test variables such as inlet air temperature, air pressure and also maltodextrin content.
25618319	1	22	theme	human	172:176	arg1	health					178:183	human health	172:183	human health	172:183	BACKGROUND There is substantial clinical data supporting the role of Bifidobacterium bifidum in human health particularly in benefiting the immune system and suppressing intestinal infections.
25618319	4	23	from	factors	618:624	arg1	drying					635:640	spray drying	629:640	spray drying of cultured bifidobacteria	629:667	The aim of this study was to establish the optimized environmental factors in spray drying of cultured bifidobacteria to obtain a viable and stable powder.
25618319	5	24	theme	inlet	771:775	arg1	temperature					781:791	inlet air temperature	771:791	inlet air temperature	771:791	METHODS The experiments were designed to test variables such as inlet air temperature, air pressure and also maltodextrin content.
25618319	1	25	from	role	137:140	arg1	health					178:183	human health	172:183	human health	172:183	BACKGROUND There is substantial clinical data supporting the role of Bifidobacterium bifidum in human health particularly in benefiting the immune system and suppressing intestinal infections.
25618319	3	26	theme	high	429:432	arg1	rates					445:449	high production rates	429:449	high production rates	429:449	The technique offers high production rates and low operating costs but is not usually used for drying of substances prone to high temperature.
25618319	10	27	theme	predicted	1510:1518	arg1	values					1520:1525	predicted values	1510:1525	predicted values from fitted correlations	1510:1550	Results of verification experiments and predicted values from fitted correlations were in close agreement at 95% confidence interval.
25618319	3	28	used	used	494:497	arg2	technique					412:420	The technique	408:420	The technique	408:420	The technique offers high production rates and low operating costs but is not usually used for drying of substances prone to high temperature.
25618319	1	29	theme	substantial	96:106	arg1	data					117:120	substantial clinical data	96:120	substantial clinical data supporting the role of Bifidobacterium bifidum in human health particularly in benefiting the immune system and suppressing intestinal infections	96:266	BACKGROUND There is substantial clinical data supporting the role of Bifidobacterium bifidum in human health particularly in benefiting the immune system and suppressing intestinal infections.
25618319	10	30	from	%	1581:1581	arg1	agreement					1566:1574	close agreement	1560:1574	close agreement	1560:1574	Results of verification experiments and predicted values from fitted correlations were in close agreement at 95% confidence interval.
25618319	12	31	theme	%	1880:1880	arg1	survival					1863:1870	the maximum survival	1851:1870	the maximum survival of 28.38%	1851:1880	Under optimum conditions, the maximum survival of 28.38% was achieved while moisture was maintained at 4.05%.
25618319	6	32	theme	moisture	897:904	arg1	content					906:912	survival rateand moisture content	880:912	survival rateand moisture content of bacterial powder	880:932	The combined effect of these variables on survival rateand moisture content of bacterial powder was studied using a central composite design (CCD).
25618319	3	33	theme	low	455:457	arg1	costs					469:473	low operating costs	455:473	low operating costs	455:473	The technique offers high production rates and low operating costs but is not usually used for drying of substances prone to high temperature.
25618319	7	34	dep	B.	1018:1019	arg1	bifidum					1021:1027	bifidum	1021:1027	bifidum	1021:1027	Sub-lethal heat-adaptation of a B. bifidum strain which was previously adapted to acid-bile-NaCl led to much more resistance to high outlet temperature during spray drying.
25618319	0	35	theme	Bifidobacterium	31:45	arg1	bifidum					47:53	Bifidobacterium bifidum	31:53	Bifidobacterium bifidum	31:53	Factors affecting viability of Bifidobacterium bifidum during spray drying.
25618319	6	36	theme	survival	880:887	arg1	content					906:912	survival rateand moisture content	880:912	survival rateand moisture content of bacterial powder	880:932	The combined effect of these variables on survival rateand moisture content of bacterial powder was studied using a central composite design (CCD).
25618319	11	37	theme	inlet	1728:1732	arg1	temperature					1738:1748	inlet air temperature	1728:1748	inlet air temperature of 111.15°C	1728:1760	The optimal values of the variables for maximum survival and minimum moisture content of B. bifidum powder were as follows: inlet air temperature of 111.15°C, air pressure of 4.5 bar and maltodextrin concentration of 6%.
25618319	11	38	theme	maximum	1644:1650	arg1	survival					1652:1659	maximum survival	1644:1659	maximum survival	1644:1659	The optimal values of the variables for maximum survival and minimum moisture content of B. bifidum powder were as follows: inlet air temperature of 111.15°C, air pressure of 4.5 bar and maltodextrin concentration of 6%.
25618319	3	39	theme	prone	524:528	arg1	substances					513:522	substances	513:522	substances prone to high temperature	513:548	The technique offers high production rates and low operating costs but is not usually used for drying of substances prone to high temperature.
25618319	5	40	theme	maltodextrin	816:827	arg1	content					829:835	maltodextrin content	816:835	maltodextrin content	816:835	METHODS The experiments were designed to test variables such as inlet air temperature, air pressure and also maltodextrin content.
25618319	12	41	theme	maximum	1855:1861	arg1	survival					1863:1870	the maximum survival	1851:1870	the maximum survival of 28.38%	1851:1880	Under optimum conditions, the maximum survival of 28.38% was achieved while moisture was maintained at 4.05%.
25618319	6	42	theme	variables	867:875	arg1	effect					851:856	The combined effect	838:856	The combined effect of these variables on survival rateand moisture content of bacterial powder	838:932	The combined effect of these variables on survival rateand moisture content of bacterial powder was studied using a central composite design (CCD).
25618319	13	43	dep	adapted	2059:2065	arg1	acid					2054:2057	acid	2054:2057	acid	2054:2057	CONCLUSION Viable and cost effective spray drying of Bifidobacterium bifidum could be achieved by cultivating heat and acid adapted strain into the culture media containing nutritional protective agents.
25618319	8	44	dep	friendly	1211:1218	arg1	permeate					1220:1227	permeate	1220:1227	permeate	1220:1227	The resistant B. bifidum was supplemented with cost friendly permeate, sucrose, yeast extract and different amount of maltodextrin before it was fed into a Buchi B-191 mini spray-dryer.
25618319	11	45	theme	air	1763:1765	arg1	pressure					1767:1774	air pressure	1763:1774	air pressure of 4.5 bar	1763:1785	The optimal values of the variables for maximum survival and minimum moisture content of B. bifidum powder were as follows: inlet air temperature of 111.15°C, air pressure of 4.5 bar and maltodextrin concentration of 6%.
25618319	11	46	theme	variables	1630:1638	arg1	values					1616:1621	The optimal values	1604:1621	The optimal values of the variables for maximum survival and minimum moisture content of B. bifidum powder	1604:1709	The optimal values of the variables for maximum survival and minimum moisture content of B. bifidum powder were as follows: inlet air temperature of 111.15°C, air pressure of 4.5 bar and maltodextrin concentration of 6%.
25618319	2	47	theme	large	365:369	arg1	quantities					371:380	large quantities	365:380	large quantities of viable microorganisms	365:405	Compared to the traditional lyophilization, spray-drying is an economical process for preparing large quantities of viable microorganisms.
25618319	4	48	theme	spray	629:633	arg1	drying					635:640	spray drying	629:640	spray drying of cultured bifidobacteria	629:667	The aim of this study was to establish the optimized environmental factors in spray drying of cultured bifidobacteria to obtain a viable and stable powder.
25618319	11	49	theme	111.15°C	1753:1760	arg1	pressure					1767:1774	air pressure	1763:1774	air pressure of 4.5 bar	1763:1785	The optimal values of the variables for maximum survival and minimum moisture content of B. bifidum powder were as follows: inlet air temperature of 111.15°C, air pressure of 4.5 bar and maltodextrin concentration of 6%.
25618319	11	49	theme	111.15°C	1753:1760	arg1	temperature					1738:1748	inlet air temperature	1728:1748	inlet air temperature of 111.15°C	1728:1760	The optimal values of the variables for maximum survival and minimum moisture content of B. bifidum powder were as follows: inlet air temperature of 111.15°C, air pressure of 4.5 bar and maltodextrin concentration of 6%.
25618319	11	49	theme	111.15°C	1753:1760	arg1	concentration					1804:1816	maltodextrin concentration	1791:1816	maltodextrin concentration of 6%	1791:1822	The optimal values of the variables for maximum survival and minimum moisture content of B. bifidum powder were as follows: inlet air temperature of 111.15°C, air pressure of 4.5 bar and maltodextrin concentration of 6%.
25618319	11	50	theme	moisture	1673:1680	arg1	content					1682:1688	minimum moisture content	1665:1688	minimum moisture content	1665:1688	The optimal values of the variables for maximum survival and minimum moisture content of B. bifidum powder were as follows: inlet air temperature of 111.15°C, air pressure of 4.5 bar and maltodextrin concentration of 6%.
25618319	11	51	theme	bifidum	1696:1702	arg1	powder					1704:1709	B. bifidum powder	1693:1709	B. bifidum powder	1693:1709	The optimal values of the variables for maximum survival and minimum moisture content of B. bifidum powder were as follows: inlet air temperature of 111.15°C, air pressure of 4.5 bar and maltodextrin concentration of 6%.
25618319	7	52	theme	spray	1145:1149	arg1	drying					1151:1156	spray drying	1145:1156	spray drying	1145:1156	Sub-lethal heat-adaptation of a B. bifidum strain which was previously adapted to acid-bile-NaCl led to much more resistance to high outlet temperature during spray drying.
25618319	2	53	theme	microorganisms	392:405	arg1	quantities					371:380	large quantities	365:380	large quantities of viable microorganisms	365:405	Compared to the traditional lyophilization, spray-drying is an economical process for preparing large quantities of viable microorganisms.
25618319	4	54	theme	optimized	594:602	arg1	factors					618:624	the optimized environmental factors	590:624	the optimized environmental factors in spray drying of cultured bifidobacteria	590:667	The aim of this study was to establish the optimized environmental factors in spray drying of cultured bifidobacteria to obtain a viable and stable powder.
25618319	6	55	theme	powder	927:932	arg1	content					906:912	survival rateand moisture content	880:912	survival rateand moisture content of bacterial powder	880:932	The combined effect of these variables on survival rateand moisture content of bacterial powder was studied using a central composite design (CCD).
25618319	7	56	theme	high	1114:1117	arg1	temperature					1126:1136	high outlet temperature	1114:1136	high outlet temperature during spray drying	1114:1156	Sub-lethal heat-adaptation of a B. bifidum strain which was previously adapted to acid-bile-NaCl led to much more resistance to high outlet temperature during spray drying.
25618319	4	57	theme	viable	681:686	arg1	powder					699:704	a viable and stable powder	679:704	a viable and stable powder	679:704	The aim of this study was to establish the optimized environmental factors in spray drying of cultured bifidobacteria to obtain a viable and stable powder.
25618319	8	58	theme	cost	1206:1209	arg1	friendly					1211:1218	cost friendly permeate	1206:1227	cost friendly permeate	1206:1227	The resistant B. bifidum was supplemented with cost friendly permeate, sucrose, yeast extract and different amount of maltodextrin before it was fed into a Buchi B-191 mini spray-dryer.
25618319	4	59	theme	stable	692:697	arg1	powder					699:704	a viable and stable powder	679:704	a viable and stable powder	679:704	The aim of this study was to establish the optimized environmental factors in spray drying of cultured bifidobacteria to obtain a viable and stable powder.
25618319	11	60	theme	optimal	1608:1614	arg1	values					1616:1621	The optimal values	1604:1621	The optimal values of the variables for maximum survival and minimum moisture content of B. bifidum powder	1604:1709	The optimal values of the variables for maximum survival and minimum moisture content of B. bifidum powder were as follows: inlet air temperature of 111.15°C, air pressure of 4.5 bar and maltodextrin concentration of 6%.
25618319	2	61	theme	economical	332:341	arg1	spray-drying					313:324	spray-drying	313:324	spray-drying	313:324	Compared to the traditional lyophilization, spray-drying is an economical process for preparing large quantities of viable microorganisms.
25618319	2	61	theme	economical	332:341	arg1	process					343:349	an economical process	329:349	an economical process for preparing large quantities of viable microorganisms	329:405	Compared to the traditional lyophilization, spray-drying is an economical process for preparing large quantities of viable microorganisms.
25618319	4	62	theme	bifidobacteria	654:667	arg1	drying					635:640	spray drying	629:640	spray drying of cultured bifidobacteria	629:667	The aim of this study was to establish the optimized environmental factors in spray drying of cultured bifidobacteria to obtain a viable and stable powder.
25618319	11	63	theme	%	1822:1822	arg1	pressure					1767:1774	air pressure	1763:1774	air pressure of 4.5 bar	1763:1785	The optimal values of the variables for maximum survival and minimum moisture content of B. bifidum powder were as follows: inlet air temperature of 111.15°C, air pressure of 4.5 bar and maltodextrin concentration of 6%.
25618319	11	63	theme	%	1822:1822	arg1	temperature					1738:1748	inlet air temperature	1728:1748	inlet air temperature of 111.15°C	1728:1760	The optimal values of the variables for maximum survival and minimum moisture content of B. bifidum powder were as follows: inlet air temperature of 111.15°C, air pressure of 4.5 bar and maltodextrin concentration of 6%.
25618319	11	63	theme	%	1822:1822	arg1	concentration					1804:1816	maltodextrin concentration	1791:1816	maltodextrin concentration of 6%	1791:1822	The optimal values of the variables for maximum survival and minimum moisture content of B. bifidum powder were as follows: inlet air temperature of 111.15°C, air pressure of 4.5 bar and maltodextrin concentration of 6%.
25618319	8	64	theme	yeast	1239:1243	arg1	extract					1245:1251	yeast extract	1239:1251	yeast extract	1239:1251	The resistant B. bifidum was supplemented with cost friendly permeate, sucrose, yeast extract and different amount of maltodextrin before it was fed into a Buchi B-191 mini spray-dryer.
25618319	6	65	theme	composite	962:970	arg1	design					972:977	a central composite design	952:977	a central composite design (CCD)	952:983	The combined effect of these variables on survival rateand moisture content of bacterial powder was studied using a central composite design (CCD).
25618319	6	65	theme	composite	962:970	arg1	CCD					980:982	CCD	980:982	CCD	980:982	The combined effect of these variables on survival rateand moisture content of bacterial powder was studied using a central composite design (CCD).
25618319	13	66	theme	effective	1962:1970	arg1	drying					1978:1983	CONCLUSION Viable and cost effective spray drying	1935:1983	CONCLUSION Viable and cost effective spray drying of Bifidobacterium bifidum	1935:2010	CONCLUSION Viable and cost effective spray drying of Bifidobacterium bifidum could be achieved by cultivating heat and acid adapted strain into the culture media containing nutritional protective agents.
25618319	13	67	theme	protective	2120:2129	arg1	agents					2131:2136	nutritional protective agents	2108:2136	nutritional protective agents	2108:2136	CONCLUSION Viable and cost effective spray drying of Bifidobacterium bifidum could be achieved by cultivating heat and acid adapted strain into the culture media containing nutritional protective agents.
25618319	2	68	theme	traditional	285:295	arg1	lyophilization					297:310	the traditional lyophilization	281:310	the traditional lyophilization	281:310	Compared to the traditional lyophilization, spray-drying is an economical process for preparing large quantities of viable microorganisms.
25618319	7	69	theme	strain	1029:1034	arg1	heat-adaptation					997:1011	Sub-lethal heat-adaptation	986:1011	Sub-lethal heat-adaptation of a B. bifidum strain which was previously adapted to acid-bile-NaCl	986:1081	Sub-lethal heat-adaptation of a B. bifidum strain which was previously adapted to acid-bile-NaCl led to much more resistance to high outlet temperature during spray drying.
25618319	10	70	from	correlations	1539:1550	arg1	Results					1470:1476	Results	1470:1476	Results of verification experiments	1470:1504	Results of verification experiments and predicted values from fitted correlations were in close agreement at 95% confidence interval.
25618319	10	70	from	correlations	1539:1550	arg1	values					1520:1525	predicted values	1510:1525	predicted values from fitted correlations	1510:1550	Results of verification experiments and predicted values from fitted correlations were in close agreement at 95% confidence interval.
25618319	11	71	theme	bar	1783:1785	arg1	pressure					1767:1774	air pressure	1763:1774	air pressure of 4.5 bar	1763:1785	The optimal values of the variables for maximum survival and minimum moisture content of B. bifidum powder were as follows: inlet air temperature of 111.15°C, air pressure of 4.5 bar and maltodextrin concentration of 6%.
25618319	11	71	theme	bar	1783:1785	arg1	temperature					1738:1748	inlet air temperature	1728:1748	inlet air temperature of 111.15°C	1728:1760	The optimal values of the variables for maximum survival and minimum moisture content of B. bifidum powder were as follows: inlet air temperature of 111.15°C, air pressure of 4.5 bar and maltodextrin concentration of 6%.
25618319	11	71	theme	bar	1783:1785	arg1	concentration					1804:1816	maltodextrin concentration	1791:1816	maltodextrin concentration of 6%	1791:1822	The optimal values of the variables for maximum survival and minimum moisture content of B. bifidum powder were as follows: inlet air temperature of 111.15°C, air pressure of 4.5 bar and maltodextrin concentration of 6%.
25618319	5	72	theme	air	777:779	arg1	temperature					781:791	inlet air temperature	771:791	inlet air temperature	771:791	METHODS The experiments were designed to test variables such as inlet air temperature, air pressure and also maltodextrin content.
25618319	7	73	theme	B.	1018:1019	arg1	strain					1029:1034	a B. bifidum strain	1016:1034	a B. bifidum strain which was previously adapted to acid-bile-NaCl	1016:1081	Sub-lethal heat-adaptation of a B. bifidum strain which was previously adapted to acid-bile-NaCl led to much more resistance to high outlet temperature during spray drying.
25618319	1	74	theme	Bifidobacterium	145:159	arg1	bifidum					161:167	Bifidobacterium bifidum	145:167	Bifidobacterium bifidum	145:167	BACKGROUND There is substantial clinical data supporting the role of Bifidobacterium bifidum in human health particularly in benefiting the immune system and suppressing intestinal infections.
25618319	13	75	theme	culture	2083:2089	arg1	media					2091:2095	the culture media	2079:2095	the culture media containing nutritional protective agents	2079:2136	CONCLUSION Viable and cost effective spray drying of Bifidobacterium bifidum could be achieved by cultivating heat and acid adapted strain into the culture media containing nutritional protective agents.
25618319	9	76	dep	RESULTS	1345:1351	arg1	established					1383:1393	established	1383:1393	were established to identify the relationship between the responses andthe three variables	1378:1467	RESULTS Second-order polynomials were established to identify the relationship between the responses andthe three variables.
25618319	10	77	theme	verification	1481:1492	arg1	experiments					1494:1504	verification experiments	1481:1504	verification experiments	1481:1504	Results of verification experiments and predicted values from fitted correlations were in close agreement at 95% confidence interval.
25618319	7	78	theme	Sub-lethal	986:995	arg1	heat-adaptation					997:1011	Sub-lethal heat-adaptation	986:1011	Sub-lethal heat-adaptation of a B. bifidum strain which was previously adapted to acid-bile-NaCl	986:1081	Sub-lethal heat-adaptation of a B. bifidum strain which was previously adapted to acid-bile-NaCl led to much more resistance to high outlet temperature during spray drying.
25618319	13	79	theme	bifidum	2004:2010	arg1	drying					1978:1983	CONCLUSION Viable and cost effective spray drying	1935:1983	CONCLUSION Viable and cost effective spray drying of Bifidobacterium bifidum	1935:2010	CONCLUSION Viable and cost effective spray drying of Bifidobacterium bifidum could be achieved by cultivating heat and acid adapted strain into the culture media containing nutritional protective agents.
25618319	11	80	theme	maltodextrin	1791:1802	arg1	concentration					1804:1816	maltodextrin concentration	1791:1816	maltodextrin concentration of 6%	1791:1822	The optimal values of the variables for maximum survival and minimum moisture content of B. bifidum powder were as follows: inlet air temperature of 111.15°C, air pressure of 4.5 bar and maltodextrin concentration of 6%.
25618319	13	81	theme	adapted	2059:2065	arg1	strain					2067:2072	acid adapted strain	2054:2072	acid adapted strain	2054:2072	CONCLUSION Viable and cost effective spray drying of Bifidobacterium bifidum could be achieved by cultivating heat and acid adapted strain into the culture media containing nutritional protective agents.
25618319	8	82	theme	maltodextrin	1277:1288	arg1	amount					1267:1272	different amount	1257:1272	different amount of maltodextrin	1257:1288	The resistant B. bifidum was supplemented with cost friendly permeate, sucrose, yeast extract and different amount of maltodextrin before it was fed into a Buchi B-191 mini spray-dryer.
25618319	8	82	theme	maltodextrin	1277:1288	arg1	maltodextrin					1277:1288	maltodextrin	1277:1288	maltodextrin	1277:1288	The resistant B. bifidum was supplemented with cost friendly permeate, sucrose, yeast extract and different amount of maltodextrin before it was fed into a Buchi B-191 mini spray-dryer.
25618319	8	82	theme	maltodextrin	1277:1288	arg1	friendly					1211:1218	cost friendly permeate	1206:1227	cost friendly permeate	1206:1227	The resistant B. bifidum was supplemented with cost friendly permeate, sucrose, yeast extract and different amount of maltodextrin before it was fed into a Buchi B-191 mini spray-dryer.
25618319	8	82	theme	maltodextrin	1277:1288	arg1	sucrose					1230:1236	sucrose	1230:1236	sucrose	1230:1236	The resistant B. bifidum was supplemented with cost friendly permeate, sucrose, yeast extract and different amount of maltodextrin before it was fed into a Buchi B-191 mini spray-dryer.
25618319	8	82	theme	maltodextrin	1277:1288	arg1	extract					1245:1251	yeast extract	1239:1251	yeast extract	1239:1251	The resistant B. bifidum was supplemented with cost friendly permeate, sucrose, yeast extract and different amount of maltodextrin before it was fed into a Buchi B-191 mini spray-dryer.
25618319	5	83	dep	METHODS	707:713	arg1	designed					736:743	designed	736:743	were designed to test variables such as inlet air temperature, air pressure and also maltodextrin content	731:835	METHODS The experiments were designed to test variables such as inlet air temperature, air pressure and also maltodextrin content.
25618319	3	84	theme	production	434:443	arg1	rates					445:449	high production rates	429:449	high production rates	429:449	The technique offers high production rates and low operating costs but is not usually used for drying of substances prone to high temperature.
25618319	9	85	theme	Second-order	1353:1364	arg1	polynomials					1366:1376	Second-order polynomials	1353:1376	Second-order polynomials	1353:1376	RESULTS Second-order polynomials were established to identify the relationship between the responses andthe three variables.
25618319	1	86	theme	clinical	108:115	arg1	data					117:120	substantial clinical data	96:120	substantial clinical data supporting the role of Bifidobacterium bifidum in human health particularly in benefiting the immune system and suppressing intestinal infections	96:266	BACKGROUND There is substantial clinical data supporting the role of Bifidobacterium bifidum in human health particularly in benefiting the immune system and suppressing intestinal infections.
25618319	11	87	theme	air	1734:1736	arg1	temperature					1738:1748	inlet air temperature	1728:1748	inlet air temperature of 111.15°C	1728:1760	The optimal values of the variables for maximum survival and minimum moisture content of B. bifidum powder were as follows: inlet air temperature of 111.15°C, air pressure of 4.5 bar and maltodextrin concentration of 6%.
25618319	0	88	theme	bifidum	47:53	arg1	viability					18:26	viability	18:26	viability of Bifidobacterium bifidum	18:53	Factors affecting viability of Bifidobacterium bifidum during spray drying.
25618319	6	89	theme	rateand	889:895	arg1	content					906:912	survival rateand moisture content	880:912	survival rateand moisture content of bacterial powder	880:932	The combined effect of these variables on survival rateand moisture content of bacterial powder was studied using a central composite design (CCD).
25618319	3	90	theme	operating	459:467	arg1	costs					469:473	low operating costs	455:473	low operating costs	455:473	The technique offers high production rates and low operating costs but is not usually used for drying of substances prone to high temperature.
25618319	10	91	theme	fitted	1532:1537	arg1	correlations					1539:1550	fitted correlations	1532:1550	fitted correlations	1532:1550	Results of verification experiments and predicted values from fitted correlations were in close agreement at 95% confidence interval.
25618319	0	92	theme	spray	62:66	arg1	drying					68:73	spray drying	62:73	spray drying	62:73	Factors affecting viability of Bifidobacterium bifidum during spray drying.
25618319	11	93	dep	were	1711:1714	arg1	pressure					1767:1774	air pressure	1763:1774	air pressure of 4.5 bar	1763:1785	The optimal values of the variables for maximum survival and minimum moisture content of B. bifidum powder were as follows: inlet air temperature of 111.15°C, air pressure of 4.5 bar and maltodextrin concentration of 6%.
25618319	11	93	dep	were	1711:1714	arg1	temperature					1738:1748	inlet air temperature	1728:1748	inlet air temperature of 111.15°C	1728:1760	The optimal values of the variables for maximum survival and minimum moisture content of B. bifidum powder were as follows: inlet air temperature of 111.15°C, air pressure of 4.5 bar and maltodextrin concentration of 6%.
25618319	11	93	dep	were	1711:1714	arg1	concentration					1804:1816	maltodextrin concentration	1791:1816	maltodextrin concentration of 6%	1791:1822	The optimal values of the variables for maximum survival and minimum moisture content of B. bifidum powder were as follows: inlet air temperature of 111.15°C, air pressure of 4.5 bar and maltodextrin concentration of 6%.
25618319	12	94	theme	optimum	1831:1837	arg1	conditions					1839:1848	optimum conditions	1831:1848	optimum conditions	1831:1848	Under optimum conditions, the maximum survival of 28.38% was achieved while moisture was maintained at 4.05%.
25618319	4	95	theme	study	567:571	arg1	aim					555:557	The aim	551:557	The aim of this study	551:571	The aim of this study was to establish the optimized environmental factors in spray drying of cultured bifidobacteria to obtain a viable and stable powder.
25618319	10	96	theme	close	1560:1564	arg1	agreement					1566:1574	close agreement	1560:1574	close agreement	1560:1574	Results of verification experiments and predicted values from fitted correlations were in close agreement at 95% confidence interval.
25618319	11	97	theme	minimum	1665:1671	arg1	content					1682:1688	minimum moisture content	1665:1688	minimum moisture content	1665:1688	The optimal values of the variables for maximum survival and minimum moisture content of B. bifidum powder were as follows: inlet air temperature of 111.15°C, air pressure of 4.5 bar and maltodextrin concentration of 6%.
25618319	3	98	theme	substances	513:522	arg1	drying					503:508	drying	503:508	drying of substances prone to high temperature	503:548	The technique offers high production rates and low operating costs but is not usually used for drying of substances prone to high temperature.
25618319	1	99	theme	intestinal	246:255	arg1	infections					257:266	intestinal infections	246:266	intestinal infections	246:266	BACKGROUND There is substantial clinical data supporting the role of Bifidobacterium bifidum in human health particularly in benefiting the immune system and suppressing intestinal infections.
25618319	4	100	theme	cultured	645:652	arg1	bifidobacteria					654:667	cultured bifidobacteria	645:667	cultured bifidobacteria	645:667	The aim of this study was to establish the optimized environmental factors in spray drying of cultured bifidobacteria to obtain a viable and stable powder.
25618319	10	101	theme	interval	1594:1601	arg1	%					1581:1581	95%	1579:1581	95% confidence interval	1579:1601	Results of verification experiments and predicted values from fitted correlations were in close agreement at 95% confidence interval.
25618319	11	102	theme	B.	1693:1694	arg1	powder					1704:1709	B. bifidum powder	1693:1709	B. bifidum powder	1693:1709	The optimal values of the variables for maximum survival and minimum moisture content of B. bifidum powder were as follows: inlet air temperature of 111.15°C, air pressure of 4.5 bar and maltodextrin concentration of 6%.
24614541	8	0	theme	systemic	1139:1146	arg1	inflammation					1148:1159	acute systemic inflammation	1133:1159	acute systemic inflammation	1133:1159	Our results indicate that individual variation in IgG glycosylation changes during acute systemic inflammation associates with increased mortality risk and indicates new avenues for the development of personalized diagnostic and therapeutic approach.
24614541	8	1	theme	personalized	1251:1262	arg1	approach					1291:1298	personalized diagnostic and therapeutic approach	1251:1298	personalized diagnostic and therapeutic approach	1251:1298	Our results indicate that individual variation in IgG glycosylation changes during acute systemic inflammation associates with increased mortality risk and indicates new avenues for the development of personalized diagnostic and therapeutic approach.
24614541	7	2	from	increase	936:943	arg1	glycoforms					963:972	galactosylated glycoforms	948:972	galactosylated glycoforms	948:972	One of them, characterized by a rapid increase in galactosylated glycoforms, was associated with nearly double mortality risk measured by EuroSCORE II.
24614541	0	3	theme	systemic	58:65	arg1	inflammation					67:78	acute systemic inflammation	52:78	acute systemic inflammation	52:78	Changes in IgG and total plasma protein glycomes in acute systemic inflammation.
24614541	4	4	theme	glycosylation	721:733	arg1	changes					735:741	glycosylation changes	721:741	glycosylation changes	721:741	In nearly all individuals plasma N-glycome underwent the same pattern of changes in the first 72 h, revealing a general mechanism of glycosylation changes.
24614541	3	5	from	composition	493:503	arg1	patients					551:558	107 patients	547:558	107 patients undergoing cardiac surgery	547:585	We analysed composition of the total plasma and IgG N-glycomes in 107 patients undergoing cardiac surgery.
24614541	1	6	theme	systemic	234:241	arg1	inflammation					243:254	systemic inflammation	234:254	systemic inflammation	234:254	Recovery after cardiac surgery is a complex process that has to compensate for both individual variability and extensive tissue damage in the context of systemic inflammation.
24614541	0	7	from	Changes	0:6	arg1	IgG					11:13	IgG	11:13	IgG	11:13	Changes in IgG and total plasma protein glycomes in acute systemic inflammation.
24614541	0	7	from	Changes	0:6	arg1	protein					32:38	total plasma protein	19:38	total plasma protein	19:38	Changes in IgG and total plasma protein glycomes in acute systemic inflammation.
24614541	3	8	theme	IgG	529:531	arg1	N-glycomes					533:542	the total plasma and IgG N-glycomes	508:542	the total plasma and IgG N-glycomes in 107 patients undergoing cardiac surgery	508:585	We analysed composition of the total plasma and IgG N-glycomes in 107 patients undergoing cardiac surgery.
24614541	2	9	from	steps	300:304	arg1	essential					282:290	essential	282:290	essential	282:290	Protein glycosylation is essential in many steps of the inflammatory cascade, but due to technological limitations the role of individual variation in glycosylation in systemic inflammation has not been addressed until now.
24614541	1	10	theme	inflammation	243:254	arg1	context					223:229	the context	219:229	the context of systemic inflammation	219:254	Recovery after cardiac surgery is a complex process that has to compensate for both individual variability and extensive tissue damage in the context of systemic inflammation.
24614541	3	11	theme	N-glycomes	533:542	arg1	composition					493:503	composition	493:503	composition of the total plasma and IgG N-glycomes in 107 patients undergoing cardiac surgery	493:585	We analysed composition of the total plasma and IgG N-glycomes in 107 patients undergoing cardiac surgery.
24614541	8	12	from	variation	1087:1095	arg1	changes					1118:1124	IgG glycosylation changes	1100:1124	IgG glycosylation changes	1100:1124	Our results indicate that individual variation in IgG glycosylation changes during acute systemic inflammation associates with increased mortality risk and indicates new avenues for the development of personalized diagnostic and therapeutic approach.
24614541	8	13	theme	increased	1177:1185	arg1	risk					1197:1200	increased mortality risk	1177:1200	increased mortality risk	1177:1200	Our results indicate that individual variation in IgG glycosylation changes during acute systemic inflammation associates with increased mortality risk and indicates new avenues for the development of personalized diagnostic and therapeutic approach.
24614541	6	14	theme	patterns	877:884	arg1	existence					850:858	the existence	846:858	the existence of four distinct patterns of changes	846:895	Bi-clustering analysis revealed the existence of four distinct patterns of changes.
24614541	8	15	theme	diagnostic	1264:1273	arg1	approach					1291:1298	personalized diagnostic and therapeutic approach	1251:1298	personalized diagnostic and therapeutic approach	1251:1298	Our results indicate that individual variation in IgG glycosylation changes during acute systemic inflammation associates with increased mortality risk and indicates new avenues for the development of personalized diagnostic and therapeutic approach.
24614541	7	16	theme	rapid	930:934	arg1	increase					936:943	a rapid increase	928:943	a rapid increase in galactosylated glycoforms	928:972	One of them, characterized by a rapid increase in galactosylated glycoforms, was associated with nearly double mortality risk measured by EuroSCORE II.
24614541	3	17	theme	plasma	518:523	arg1	N-glycomes					533:542	the total plasma and IgG N-glycomes	508:542	the total plasma and IgG N-glycomes in 107 patients undergoing cardiac surgery	508:585	We analysed composition of the total plasma and IgG N-glycomes in 107 patients undergoing cardiac surgery.
24614541	3	18	from	patients	551:558	arg1	composition					493:503	composition	493:503	composition of the total plasma and IgG N-glycomes in 107 patients undergoing cardiac surgery	493:585	We analysed composition of the total plasma and IgG N-glycomes in 107 patients undergoing cardiac surgery.
24614541	6	19	theme	distinct	868:875	arg1	patterns					877:884	four distinct patterns	863:884	four distinct patterns of changes	863:895	Bi-clustering analysis revealed the existence of four distinct patterns of changes.
24614541	2	20	theme	Protein	257:263	arg1	glycosylation					265:277	Protein glycosylation	257:277	Protein glycosylation	257:277	Protein glycosylation is essential in many steps of the inflammatory cascade, but due to technological limitations the role of individual variation in glycosylation in systemic inflammation has not been addressed until now.
24614541	5	21	theme	IgG	776:778	arg1	glycome					780:786	the IgG glycome	772:786	the IgG glycome	772:786	To the contrary, changes in the IgG glycome were very individualized.
24614541	8	22	theme	acute	1133:1137	arg1	inflammation					1148:1159	acute systemic inflammation	1133:1159	acute systemic inflammation	1133:1159	Our results indicate that individual variation in IgG glycosylation changes during acute systemic inflammation associates with increased mortality risk and indicates new avenues for the development of personalized diagnostic and therapeutic approach.
24614541	4	23	from	pattern	650:656	arg1	h					685:685	the first 72 h	672:685	the first 72 h	672:685	In nearly all individuals plasma N-glycome underwent the same pattern of changes in the first 72 h, revealing a general mechanism of glycosylation changes.
24614541	4	24	theme	same	645:648	arg1	pattern					650:656	the same pattern	641:656	the same pattern of changes in the first 72 h	641:685	In nearly all individuals plasma N-glycome underwent the same pattern of changes in the first 72 h, revealing a general mechanism of glycosylation changes.
24614541	8	25	theme	mortality	1187:1195	arg1	risk					1197:1200	increased mortality risk	1177:1200	increased mortality risk	1177:1200	Our results indicate that individual variation in IgG glycosylation changes during acute systemic inflammation associates with increased mortality risk and indicates new avenues for the development of personalized diagnostic and therapeutic approach.
24614541	7	26	theme	them	905:908	arg1	them					905:908	them	905:908	them	905:908	One of them, characterized by a rapid increase in galactosylated glycoforms, was associated with nearly double mortality risk measured by EuroSCORE II.
24614541	7	26	theme	them	905:908	arg1	One					898:900	One	898:900	One	898:900	One of them, characterized by a rapid increase in galactosylated glycoforms, was associated with nearly double mortality risk measured by EuroSCORE II.
24614541	1	27	theme	individual	165:174	arg1	variability					176:186	individual variability	165:186	individual variability	165:186	Recovery after cardiac surgery is a complex process that has to compensate for both individual variability and extensive tissue damage in the context of systemic inflammation.
24614541	2	28	theme	systemic	425:432	arg1	inflammation					434:445	systemic inflammation	425:445	systemic inflammation	425:445	Protein glycosylation is essential in many steps of the inflammatory cascade, but due to technological limitations the role of individual variation in glycosylation in systemic inflammation has not been addressed until now.
24614541	2	29	theme	many	295:298	arg1	steps					300:304	many steps	295:304	many steps of the inflammatory cascade	295:332	Protein glycosylation is essential in many steps of the inflammatory cascade, but due to technological limitations the role of individual variation in glycosylation in systemic inflammation has not been addressed until now.
24614541	6	30	theme	changes	889:895	arg1	patterns					877:884	four distinct patterns	863:884	four distinct patterns of changes	863:895	Bi-clustering analysis revealed the existence of four distinct patterns of changes.
24614541	7	31	theme	galactosylated	948:961	arg1	glycoforms					963:972	galactosylated glycoforms	948:972	galactosylated glycoforms	948:972	One of them, characterized by a rapid increase in galactosylated glycoforms, was associated with nearly double mortality risk measured by EuroSCORE II.
24614541	1	32	theme	cardiac	96:102	arg1	surgery					104:110	cardiac surgery	96:110	cardiac surgery	96:110	Recovery after cardiac surgery is a complex process that has to compensate for both individual variability and extensive tissue damage in the context of systemic inflammation.
24614541	4	33	theme	first	676:680	arg1	h					685:685	the first 72 h	672:685	the first 72 h	672:685	In nearly all individuals plasma N-glycome underwent the same pattern of changes in the first 72 h, revealing a general mechanism of glycosylation changes.
24614541	0	34	theme	plasma	25:30	arg1	protein					32:38	total plasma protein	19:38	total plasma protein	19:38	Changes in IgG and total plasma protein glycomes in acute systemic inflammation.
24614541	4	35	from	changes	661:667	arg1	h					685:685	the first 72 h	672:685	the first 72 h	672:685	In nearly all individuals plasma N-glycome underwent the same pattern of changes in the first 72 h, revealing a general mechanism of glycosylation changes.
24614541	8	36	theme	individual	1076:1085	arg1	variation					1087:1095	individual variation	1076:1095	individual variation in IgG glycosylation changes during acute systemic inflammation	1076:1159	Our results indicate that individual variation in IgG glycosylation changes during acute systemic inflammation associates with increased mortality risk and indicates new avenues for the development of personalized diagnostic and therapeutic approach.
24614541	1	37	theme	extensive	192:200	arg1	damage					209:214	extensive tissue damage	192:214	extensive tissue damage	192:214	Recovery after cardiac surgery is a complex process that has to compensate for both individual variability and extensive tissue damage in the context of systemic inflammation.
24614541	4	38	theme	changes	661:667	arg1	pattern					650:656	the same pattern	641:656	the same pattern of changes in the first 72 h	641:685	In nearly all individuals plasma N-glycome underwent the same pattern of changes in the first 72 h, revealing a general mechanism of glycosylation changes.
24614541	2	39	theme	variation	395:403	arg1	role					376:379	the role	372:379	the role of individual variation in glycosylation in systemic inflammation	372:445	Protein glycosylation is essential in many steps of the inflammatory cascade, but due to technological limitations the role of individual variation in glycosylation in systemic inflammation has not been addressed until now.
24614541	0	40	theme	total	19:23	arg1	protein					32:38	total plasma protein	19:38	total plasma protein	19:38	Changes in IgG and total plasma protein glycomes in acute systemic inflammation.
24614541	8	41	theme	new	1216:1218	arg1	avenues					1220:1226	new avenues	1216:1226	new avenues for the development of personalized diagnostic and therapeutic approach	1216:1298	Our results indicate that individual variation in IgG glycosylation changes during acute systemic inflammation associates with increased mortality risk and indicates new avenues for the development of personalized diagnostic and therapeutic approach.
24614541	1	42	theme	tissue	202:207	arg1	damage					209:214	extensive tissue damage	192:214	extensive tissue damage	192:214	Recovery after cardiac surgery is a complex process that has to compensate for both individual variability and extensive tissue damage in the context of systemic inflammation.
24614541	3	43	from	N-glycomes	533:542	arg1	patients					551:558	107 patients	547:558	107 patients undergoing cardiac surgery	547:585	We analysed composition of the total plasma and IgG N-glycomes in 107 patients undergoing cardiac surgery.
24614541	3	44	theme	cardiac	571:577	arg1	surgery					579:585	cardiac surgery	571:585	cardiac surgery	571:585	We analysed composition of the total plasma and IgG N-glycomes in 107 patients undergoing cardiac surgery.
24614541	7	45	theme	double	1002:1007	arg1	risk					1019:1022	nearly double mortality risk	995:1022	nearly double mortality risk measured by EuroSCORE II	995:1047	One of them, characterized by a rapid increase in galactosylated glycoforms, was associated with nearly double mortality risk measured by EuroSCORE II.
24614541	5	46	from	changes	761:767	arg1	glycome					780:786	the IgG glycome	772:786	the IgG glycome	772:786	To the contrary, changes in the IgG glycome were very individualized.
24614541	2	47	theme	cascade	326:332	arg1	steps					300:304	many steps	295:304	many steps of the inflammatory cascade	295:332	Protein glycosylation is essential in many steps of the inflammatory cascade, but due to technological limitations the role of individual variation in glycosylation in systemic inflammation has not been addressed until now.
24614541	7	48	theme	mortality	1009:1017	arg1	risk					1019:1022	nearly double mortality risk	995:1022	nearly double mortality risk measured by EuroSCORE II	995:1047	One of them, characterized by a rapid increase in galactosylated glycoforms, was associated with nearly double mortality risk measured by EuroSCORE II.
24614541	6	49	theme	Bi-clustering	814:826	arg1	analysis					828:835	Bi-clustering analysis	814:835	Bi-clustering analysis	814:835	Bi-clustering analysis revealed the existence of four distinct patterns of changes.
24614541	8	50	theme	glycosylation	1104:1116	arg1	changes					1118:1124	IgG glycosylation changes	1100:1124	IgG glycosylation changes	1100:1124	Our results indicate that individual variation in IgG glycosylation changes during acute systemic inflammation associates with increased mortality risk and indicates new avenues for the development of personalized diagnostic and therapeutic approach.
24614541	2	51	from	role	376:379	arg1	inflammation					434:445	systemic inflammation	425:445	systemic inflammation	425:445	Protein glycosylation is essential in many steps of the inflammatory cascade, but due to technological limitations the role of individual variation in glycosylation in systemic inflammation has not been addressed until now.
24614541	2	51	from	role	376:379	arg1	glycosylation					408:420	glycosylation	408:420	glycosylation	408:420	Protein glycosylation is essential in many steps of the inflammatory cascade, but due to technological limitations the role of individual variation in glycosylation in systemic inflammation has not been addressed until now.
24614541	2	52	theme	inflammatory	313:324	arg1	cascade					326:332	the inflammatory cascade	309:332	the inflammatory cascade	309:332	Protein glycosylation is essential in many steps of the inflammatory cascade, but due to technological limitations the role of individual variation in glycosylation in systemic inflammation has not been addressed until now.
24614541	4	53	theme	changes	735:741	arg1	mechanism					708:716	a general mechanism	698:716	a general mechanism of glycosylation changes	698:741	In nearly all individuals plasma N-glycome underwent the same pattern of changes in the first 72 h, revealing a general mechanism of glycosylation changes.
24614541	8	54	theme	approach	1291:1298	arg1	development					1236:1246	the development	1232:1246	the development of personalized diagnostic and therapeutic approach	1232:1298	Our results indicate that individual variation in IgG glycosylation changes during acute systemic inflammation associates with increased mortality risk and indicates new avenues for the development of personalized diagnostic and therapeutic approach.
24614541	8	55	theme	IgG	1100:1102	arg1	changes					1118:1124	IgG glycosylation changes	1100:1124	IgG glycosylation changes	1100:1124	Our results indicate that individual variation in IgG glycosylation changes during acute systemic inflammation associates with increased mortality risk and indicates new avenues for the development of personalized diagnostic and therapeutic approach.
24614541	2	56	from	variation	395:403	arg1	glycosylation					408:420	glycosylation	408:420	glycosylation	408:420	Protein glycosylation is essential in many steps of the inflammatory cascade, but due to technological limitations the role of individual variation in glycosylation in systemic inflammation has not been addressed until now.
24614541	1	57	theme	complex	117:123	arg1	Recovery					81:88	Recovery	81:88	Recovery after cardiac surgery	81:110	Recovery after cardiac surgery is a complex process that has to compensate for both individual variability and extensive tissue damage in the context of systemic inflammation.
24614541	1	57	theme	complex	117:123	arg1	process					125:131	a complex process	115:131	a complex process that has to compensate for both individual variability and extensive tissue damage in the context of systemic inflammation	115:254	Recovery after cardiac surgery is a complex process that has to compensate for both individual variability and extensive tissue damage in the context of systemic inflammation.
24614541	2	58	from	essential	282:290	arg1	steps					300:304	many steps	295:304	many steps of the inflammatory cascade	295:332	Protein glycosylation is essential in many steps of the inflammatory cascade, but due to technological limitations the role of individual variation in glycosylation in systemic inflammation has not been addressed until now.
24614541	4	59	theme	general	700:706	arg1	mechanism					708:716	a general mechanism	698:716	a general mechanism of glycosylation changes	698:741	In nearly all individuals plasma N-glycome underwent the same pattern of changes in the first 72 h, revealing a general mechanism of glycosylation changes.
24614541	0	60	theme	acute	52:56	arg1	inflammation					67:78	acute systemic inflammation	52:78	acute systemic inflammation	52:78	Changes in IgG and total plasma protein glycomes in acute systemic inflammation.
24614541	2	61	theme	individual	384:393	arg1	variation					395:403	individual variation	384:403	individual variation in glycosylation	384:420	Protein glycosylation is essential in many steps of the inflammatory cascade, but due to technological limitations the role of individual variation in glycosylation in systemic inflammation has not been addressed until now.
24614541	8	62	theme	therapeutic	1279:1289	arg1	approach					1291:1298	personalized diagnostic and therapeutic approach	1251:1298	personalized diagnostic and therapeutic approach	1251:1298	Our results indicate that individual variation in IgG glycosylation changes during acute systemic inflammation associates with increased mortality risk and indicates new avenues for the development of personalized diagnostic and therapeutic approach.
24614541	2	63	theme	technological	346:358	arg1	limitations					360:370	technological limitations	346:370	technological limitations	346:370	Protein glycosylation is essential in many steps of the inflammatory cascade, but due to technological limitations the role of individual variation in glycosylation in systemic inflammation has not been addressed until now.
24614541	4	64	theme	plasma	614:619	arg1	N-glycome					621:629	plasma N-glycome	614:629	plasma N-glycome	614:629	In nearly all individuals plasma N-glycome underwent the same pattern of changes in the first 72 h, revealing a general mechanism of glycosylation changes.
24614541	2	65	from	glycosylation	408:420	arg1	role					376:379	the role	372:379	the role of individual variation in glycosylation in systemic inflammation	372:445	Protein glycosylation is essential in many steps of the inflammatory cascade, but due to technological limitations the role of individual variation in glycosylation in systemic inflammation has not been addressed until now.
24614541	3	66	theme	total	512:516	arg1	N-glycomes					533:542	the total plasma and IgG N-glycomes	508:542	the total plasma and IgG N-glycomes in 107 patients undergoing cardiac surgery	508:585	We analysed composition of the total plasma and IgG N-glycomes in 107 patients undergoing cardiac surgery.
24614541	4	67	from	h	685:685	arg1	pattern					650:656	the same pattern	641:656	the same pattern of changes in the first 72 h	641:685	In nearly all individuals plasma N-glycome underwent the same pattern of changes in the first 72 h, revealing a general mechanism of glycosylation changes.
30060403	5	0	theme	ChCl-formic	700:710	arg1	acid					712:715	ChCl-formic acid	700:715	ChCl-formic acid	700:715	In contrast, ChCl-formic acid resulted in the greatest level of glucan retention, at 97.8%, with a lignin removal rate of 40.7% under the same pretreatment conditions.
30060403	6	1	theme	ChCl	865:868	arg1	loading					870:876	ChCl loading	865:876	ChCl loading	865:876	Moreover, ChCl loading could significantly enhance the selectivity of carboxylic acid for lignin dissolution.
30060403	7	2	theme	%	1029:1029	arg1	yield					1039:1043	a 100% ethanol yield	1024:1043	a 100% ethanol yield	1024:1043	A 98.0% level of subsequent enzymatic saccharification and a 100% ethanol yield were achieved after ChCl-formic acid pretreatments of Akebia' herbal residues.
30060403	4	3	from	120°C	640:644	arg1	%					610:610	100%	607:610	100%	607:610	Glycolic acid resulted in the maximum levels of lignin, xylan and glucan removal, which were 60.0%, 100% and 71.5%, respectively, at 120°C with a 1:6M ratio of ChCl-glycolic acid.
30060403	4	3	from	120°C	640:644	arg1	%					620:620	71.5%	616:620	71.5%	616:620	Glycolic acid resulted in the maximum levels of lignin, xylan and glucan removal, which were 60.0%, 100% and 71.5%, respectively, at 120°C with a 1:6M ratio of ChCl-glycolic acid.
30060403	4	3	from	120°C	640:644	arg1	%					604:604	60.0%	600:604	60.0%	600:604	Glycolic acid resulted in the maximum levels of lignin, xylan and glucan removal, which were 60.0%, 100% and 71.5%, respectively, at 120°C with a 1:6M ratio of ChCl-glycolic acid.
30060403	4	4	theme	glucan	573:578	arg1	removal					580:586	glucan removal	573:586	glucan removal	573:586	Glycolic acid resulted in the maximum levels of lignin, xylan and glucan removal, which were 60.0%, 100% and 71.5%, respectively, at 120°C with a 1:6M ratio of ChCl-glycolic acid.
30060403	7	5	theme	ethanol	1031:1037	arg1	yield					1039:1043	a 100% ethanol yield	1024:1043	a 100% ethanol yield	1024:1043	A 98.0% level of subsequent enzymatic saccharification and a 100% ethanol yield were achieved after ChCl-formic acid pretreatments of Akebia' herbal residues.
30060403	1	6	theme	herbal	147:152	arg1	Akebia					166:171	Akebia	166:171	Akebia	166:171	Here, the potential use of herbal residues of Akebia as feedstock for ethanol production is evaluated.
30060403	1	6	theme	herbal	147:152	arg1	residues					154:161	herbal residues	147:161	herbal residues of Akebia	147:171	Here, the potential use of herbal residues of Akebia as feedstock for ethanol production is evaluated.
30060403	3	7	theme	more	373:376	arg1	influences					390:399	more significant influences	373:399	more significant influences	373:399	Reaction temperatures had more significant influences on solid loss and chemical composition than the molar ratios of choline chloride (ChCl) to derived acids.
30060403	3	8	theme	significant	378:388	arg1	influences					390:399	more significant influences	373:399	more significant influences	373:399	Reaction temperatures had more significant influences on solid loss and chemical composition than the molar ratios of choline chloride (ChCl) to derived acids.
30060403	7	9	theme	100	1026:1028	arg1	%					1029:1029	%	1029:1029	%	1029:1029	A 98.0% level of subsequent enzymatic saccharification and a 100% ethanol yield were achieved after ChCl-formic acid pretreatments of Akebia' herbal residues.
30060403	2	10	theme	hemicellulose-derived	270:290	arg1	acids					292:296	hemicellulose-derived acids	270:296	hemicellulose-derived acids	270:296	Additionally, five deep eutectic solvents from hemicellulose-derived acids were prepared to overcome biomass recalcitrance.
30060403	4	11	theme	removal	580:586	arg1	levels					545:550	the maximum levels	533:550	the maximum levels of lignin, xylan and glucan removal, which were 60.0%, 100% and 71.5%, respectively, at 120°C with a 1:6M ratio of ChCl-glycolic acid	533:684	Glycolic acid resulted in the maximum levels of lignin, xylan and glucan removal, which were 60.0%, 100% and 71.5%, respectively, at 120°C with a 1:6M ratio of ChCl-glycolic acid.
30060403	4	12	theme	1:6M	653:656	arg1	ratio					658:662	a 1:6M ratio	651:662	a 1:6M ratio of ChCl-glycolic acid	651:684	Glycolic acid resulted in the maximum levels of lignin, xylan and glucan removal, which were 60.0%, 100% and 71.5%, respectively, at 120°C with a 1:6M ratio of ChCl-glycolic acid.
30060403	7	13	theme	saccharification	1003:1018	arg1	yield					1039:1043	a 100% ethanol yield	1024:1043	a 100% ethanol yield	1024:1043	A 98.0% level of subsequent enzymatic saccharification and a 100% ethanol yield were achieved after ChCl-formic acid pretreatments of Akebia' herbal residues.
30060403	7	13	theme	saccharification	1003:1018	arg1	level					973:977	A 98.0% level	965:977	A 98.0% level of subsequent enzymatic saccharification	965:1018	A 98.0% level of subsequent enzymatic saccharification and a 100% ethanol yield were achieved after ChCl-formic acid pretreatments of Akebia' herbal residues.
30060403	1	14	theme	residues	154:161	arg1	use					140:142	the potential use	126:142	the potential use of herbal residues of Akebia as feedstock for ethanol production	126:207	Here, the potential use of herbal residues of Akebia as feedstock for ethanol production is evaluated.
30060403	0	15	theme	herbal	103:108	arg1	residues					110:117	Akebia' herbal residues	95:117	Akebia' herbal residues	95:117	Deep eutectic solvents from hemicellulose-derived acids for the cellulosic ethanol refining of Akebia' herbal residues.
30060403	0	16	from	acids	50:54	arg1	solvents					14:21	Deep eutectic solvents	0:21	Deep eutectic solvents from hemicellulose-derived acids for the cellulosic ethanol refining of Akebia' herbal residues.	0:118	Deep eutectic solvents from hemicellulose-derived acids for the cellulosic ethanol refining of Akebia' herbal residues.
30060403	5	17	theme	pretreatment	830:841	arg1	conditions					843:852	the same pretreatment conditions	821:852	the same pretreatment conditions	821:852	In contrast, ChCl-formic acid resulted in the greatest level of glucan retention, at 97.8%, with a lignin removal rate of 40.7% under the same pretreatment conditions.
30060403	0	18	theme	eutectic	5:12	arg1	solvents					14:21	Deep eutectic solvents	0:21	Deep eutectic solvents from hemicellulose-derived acids for the cellulosic ethanol refining of Akebia' herbal residues.	0:118	Deep eutectic solvents from hemicellulose-derived acids for the cellulosic ethanol refining of Akebia' herbal residues.
30060403	7	19	theme	subsequent	982:991	arg1	saccharification					1003:1018	subsequent enzymatic saccharification	982:1018	subsequent enzymatic saccharification	982:1018	A 98.0% level of subsequent enzymatic saccharification and a 100% ethanol yield were achieved after ChCl-formic acid pretreatments of Akebia' herbal residues.
30060403	4	20	theme	acid	681:684	arg1	ratio					658:662	a 1:6M ratio	651:662	a 1:6M ratio of ChCl-glycolic acid	651:684	Glycolic acid resulted in the maximum levels of lignin, xylan and glucan removal, which were 60.0%, 100% and 71.5%, respectively, at 120°C with a 1:6M ratio of ChCl-glycolic acid.
30060403	4	21	with	120°C	640:644	arg1	ratio					658:662	a 1:6M ratio	651:662	a 1:6M ratio of ChCl-glycolic acid	651:684	Glycolic acid resulted in the maximum levels of lignin, xylan and glucan removal, which were 60.0%, 100% and 71.5%, respectively, at 120°C with a 1:6M ratio of ChCl-glycolic acid.
30060403	1	22	theme	Akebia	166:171	arg1	Akebia					166:171	Akebia	166:171	Akebia	166:171	Here, the potential use of herbal residues of Akebia as feedstock for ethanol production is evaluated.
30060403	1	22	theme	Akebia	166:171	arg1	residues					154:161	herbal residues	147:161	herbal residues of Akebia	147:171	Here, the potential use of herbal residues of Akebia as feedstock for ethanol production is evaluated.
30060403	0	23	theme	Deep	0:3	arg1	solvents					14:21	Deep eutectic solvents	0:21	Deep eutectic solvents from hemicellulose-derived acids for the cellulosic ethanol refining of Akebia' herbal residues.	0:118	Deep eutectic solvents from hemicellulose-derived acids for the cellulosic ethanol refining of Akebia' herbal residues.
30060403	4	24	theme	lignin	555:560	arg1	levels					545:550	the maximum levels	533:550	the maximum levels of lignin, xylan and glucan removal, which were 60.0%, 100% and 71.5%, respectively, at 120°C with a 1:6M ratio of ChCl-glycolic acid	533:684	Glycolic acid resulted in the maximum levels of lignin, xylan and glucan removal, which were 60.0%, 100% and 71.5%, respectively, at 120°C with a 1:6M ratio of ChCl-glycolic acid.
30060403	7	25	theme	enzymatic	993:1001	arg1	saccharification					1003:1018	subsequent enzymatic saccharification	982:1018	subsequent enzymatic saccharification	982:1018	A 98.0% level of subsequent enzymatic saccharification and a 100% ethanol yield were achieved after ChCl-formic acid pretreatments of Akebia' herbal residues.
30060403	4	26	theme	ChCl-glycolic	667:679	arg1	acid					681:684	ChCl-glycolic acid	667:684	ChCl-glycolic acid	667:684	Glycolic acid resulted in the maximum levels of lignin, xylan and glucan removal, which were 60.0%, 100% and 71.5%, respectively, at 120°C with a 1:6M ratio of ChCl-glycolic acid.
30060403	3	27	theme	molar	449:453	arg1	ratios					455:460	the molar ratios	445:460	the molar ratios of choline chloride (ChCl) to derived acids	445:504	Reaction temperatures had more significant influences on solid loss and chemical composition than the molar ratios of choline chloride (ChCl) to derived acids.
30060403	5	28	theme	glucan	751:756	arg1	retention					758:766	glucan retention	751:766	glucan retention	751:766	In contrast, ChCl-formic acid resulted in the greatest level of glucan retention, at 97.8%, with a lignin removal rate of 40.7% under the same pretreatment conditions.
30060403	0	29	link	hemicellulose-derived	28:48	arg1	acids					50:54	hemicellulose-derived acids	28:54	hemicellulose-derived acids	28:54	Deep eutectic solvents from hemicellulose-derived acids for the cellulosic ethanol refining of Akebia' herbal residues.
30060403	5	30	theme	retention	758:766	arg1	level					742:746	the greatest level	729:746	the greatest level of glucan retention	729:766	In contrast, ChCl-formic acid resulted in the greatest level of glucan retention, at 97.8%, with a lignin removal rate of 40.7% under the same pretreatment conditions.
30060403	4	31	theme	xylan	563:567	arg1	levels					545:550	the maximum levels	533:550	the maximum levels of lignin, xylan and glucan removal, which were 60.0%, 100% and 71.5%, respectively, at 120°C with a 1:6M ratio of ChCl-glycolic acid	533:684	Glycolic acid resulted in the maximum levels of lignin, xylan and glucan removal, which were 60.0%, 100% and 71.5%, respectively, at 120°C with a 1:6M ratio of ChCl-glycolic acid.
30060403	5	32	theme	removal	793:799	arg1	rate					801:804	a lignin removal rate	784:804	a lignin removal rate of 40.7% under the same pretreatment conditions	784:852	In contrast, ChCl-formic acid resulted in the greatest level of glucan retention, at 97.8%, with a lignin removal rate of 40.7% under the same pretreatment conditions.
30060403	2	33	link	hemicellulose-derived	270:290	arg1	acids					292:296	hemicellulose-derived acids	270:296	hemicellulose-derived acids	270:296	Additionally, five deep eutectic solvents from hemicellulose-derived acids were prepared to overcome biomass recalcitrance.
30060403	7	34	theme	98.0	967:970	arg1	%					971:971	%	971:971	%	971:971	A 98.0% level of subsequent enzymatic saccharification and a 100% ethanol yield were achieved after ChCl-formic acid pretreatments of Akebia' herbal residues.
30060403	1	35	theme	ethanol	190:196	arg1	production					198:207	ethanol production	190:207	ethanol production	190:207	Here, the potential use of herbal residues of Akebia as feedstock for ethanol production is evaluated.
30060403	5	36	theme	lignin	786:791	arg1	rate					801:804	a lignin removal rate	784:804	a lignin removal rate of 40.7% under the same pretreatment conditions	784:852	In contrast, ChCl-formic acid resulted in the greatest level of glucan retention, at 97.8%, with a lignin removal rate of 40.7% under the same pretreatment conditions.
30060403	3	37	theme	choline	465:471	arg1	chloride					473:480	choline chloride	465:480	choline chloride (ChCl) to derived acids	465:504	Reaction temperatures had more significant influences on solid loss and chemical composition than the molar ratios of choline chloride (ChCl) to derived acids.
30060403	3	37	theme	choline	465:471	arg1	ChCl					483:486	ChCl	483:486	ChCl	483:486	Reaction temperatures had more significant influences on solid loss and chemical composition than the molar ratios of choline chloride (ChCl) to derived acids.
30060403	0	38	theme	hemicellulose-derived	28:48	arg1	acids					50:54	hemicellulose-derived acids	28:54	hemicellulose-derived acids	28:54	Deep eutectic solvents from hemicellulose-derived acids for the cellulosic ethanol refining of Akebia' herbal residues.
30060403	7	39	theme	%	971:971	arg1	level					973:977	A 98.0% level	965:977	A 98.0% level of subsequent enzymatic saccharification	965:1018	A 98.0% level of subsequent enzymatic saccharification and a 100% ethanol yield were achieved after ChCl-formic acid pretreatments of Akebia' herbal residues.
30060403	7	40	theme	ChCl-formic	1065:1075	arg1	pretreatments					1082:1094	ChCl-formic acid pretreatments	1065:1094	ChCl-formic acid pretreatments of Akebia' herbal residues	1065:1121	A 98.0% level of subsequent enzymatic saccharification and a 100% ethanol yield were achieved after ChCl-formic acid pretreatments of Akebia' herbal residues.
30060403	3	41	contain	had	369:371	arg2	influences					390:399	more significant influences	373:399	more significant influences	373:399	Reaction temperatures had more significant influences on solid loss and chemical composition than the molar ratios of choline chloride (ChCl) to derived acids.
30060403	3	41	contain	had	369:371	arg1	temperatures					356:367	Reaction temperatures	347:367	Reaction temperatures	347:367	Reaction temperatures had more significant influences on solid loss and chemical composition than the molar ratios of choline chloride (ChCl) to derived acids.
30060403	3	42	theme	chloride	473:480	arg1	ratios					455:460	the molar ratios	445:460	the molar ratios of choline chloride (ChCl) to derived acids	445:504	Reaction temperatures had more significant influences on solid loss and chemical composition than the molar ratios of choline chloride (ChCl) to derived acids.
30060403	7	43	theme	herbal	1107:1112	arg1	residues					1114:1121	Akebia' herbal residues	1099:1121	Akebia' herbal residues	1099:1121	A 98.0% level of subsequent enzymatic saccharification and a 100% ethanol yield were achieved after ChCl-formic acid pretreatments of Akebia' herbal residues.
30060403	5	44	theme	greatest	733:740	arg1	level					742:746	the greatest level	729:746	the greatest level of glucan retention	729:766	In contrast, ChCl-formic acid resulted in the greatest level of glucan retention, at 97.8%, with a lignin removal rate of 40.7% under the same pretreatment conditions.
30060403	0	45	theme	cellulosic	64:73	arg1	ethanol					75:81	the cellulosic ethanol	60:81	the cellulosic ethanol refining of Akebia' herbal residues	60:117	Deep eutectic solvents from hemicellulose-derived acids for the cellulosic ethanol refining of Akebia' herbal residues.
30060403	3	46	theme	derived	492:498	arg1	acids					500:504	derived acids	492:504	derived acids	492:504	Reaction temperatures had more significant influences on solid loss and chemical composition than the molar ratios of choline chloride (ChCl) to derived acids.
30060403	3	47	theme	chemical	419:426	arg1	composition					428:438	chemical composition	419:438	chemical composition	419:438	Reaction temperatures had more significant influences on solid loss and chemical composition than the molar ratios of choline chloride (ChCl) to derived acids.
30060403	4	48	theme	maximum	537:543	arg1	levels					545:550	the maximum levels	533:550	the maximum levels of lignin, xylan and glucan removal, which were 60.0%, 100% and 71.5%, respectively, at 120°C with a 1:6M ratio of ChCl-glycolic acid	533:684	Glycolic acid resulted in the maximum levels of lignin, xylan and glucan removal, which were 60.0%, 100% and 71.5%, respectively, at 120°C with a 1:6M ratio of ChCl-glycolic acid.
30060403	3	49	link	derived	492:498	arg1	acids					500:504	derived acids	492:504	derived acids	492:504	Reaction temperatures had more significant influences on solid loss and chemical composition than the molar ratios of choline chloride (ChCl) to derived acids.
30060403	2	50	theme	eutectic	247:254	arg1	solvents					256:263	five deep eutectic solvents	237:263	five deep eutectic solvents from hemicellulose-derived acids	237:296	Additionally, five deep eutectic solvents from hemicellulose-derived acids were prepared to overcome biomass recalcitrance.
30060403	6	51	theme	acid	936:939	arg1	selectivity					910:920	the selectivity	906:920	the selectivity of carboxylic acid for lignin dissolution	906:962	Moreover, ChCl loading could significantly enhance the selectivity of carboxylic acid for lignin dissolution.
30060403	5	52	theme	%	813:813	arg1	rate					801:804	a lignin removal rate	784:804	a lignin removal rate of 40.7% under the same pretreatment conditions	784:852	In contrast, ChCl-formic acid resulted in the greatest level of glucan retention, at 97.8%, with a lignin removal rate of 40.7% under the same pretreatment conditions.
30060403	2	53	theme	deep	242:245	arg1	solvents					256:263	five deep eutectic solvents	237:263	five deep eutectic solvents from hemicellulose-derived acids	237:296	Additionally, five deep eutectic solvents from hemicellulose-derived acids were prepared to overcome biomass recalcitrance.
30060403	2	54	theme	biomass	324:330	arg1	recalcitrance					332:344	biomass recalcitrance	324:344	biomass recalcitrance	324:344	Additionally, five deep eutectic solvents from hemicellulose-derived acids were prepared to overcome biomass recalcitrance.
30060403	2	55	from	acids	292:296	arg1	solvents					256:263	five deep eutectic solvents	237:263	five deep eutectic solvents from hemicellulose-derived acids	237:296	Additionally, five deep eutectic solvents from hemicellulose-derived acids were prepared to overcome biomass recalcitrance.
30060403	5	56	theme	same	825:828	arg1	conditions					843:852	the same pretreatment conditions	821:852	the same pretreatment conditions	821:852	In contrast, ChCl-formic acid resulted in the greatest level of glucan retention, at 97.8%, with a lignin removal rate of 40.7% under the same pretreatment conditions.
30060403	6	57	theme	lignin	945:950	arg1	dissolution					952:962	lignin dissolution	945:962	lignin dissolution	945:962	Moreover, ChCl loading could significantly enhance the selectivity of carboxylic acid for lignin dissolution.
30060403	1	58	theme	potential	130:138	arg1	use					140:142	the potential use	126:142	the potential use of herbal residues of Akebia as feedstock for ethanol production	126:207	Here, the potential use of herbal residues of Akebia as feedstock for ethanol production is evaluated.
30060403	6	59	theme	carboxylic	925:934	arg1	acid					936:939	carboxylic acid	925:939	carboxylic acid	925:939	Moreover, ChCl loading could significantly enhance the selectivity of carboxylic acid for lignin dissolution.
30060403	3	60	theme	Reaction	347:354	arg1	temperatures					356:367	Reaction temperatures	347:367	Reaction temperatures	347:367	Reaction temperatures had more significant influences on solid loss and chemical composition than the molar ratios of choline chloride (ChCl) to derived acids.
30060403	4	61	theme	Glycolic	507:514	arg1	acid					516:519	Glycolic acid	507:519	Glycolic acid	507:519	Glycolic acid resulted in the maximum levels of lignin, xylan and glucan removal, which were 60.0%, 100% and 71.5%, respectively, at 120°C with a 1:6M ratio of ChCl-glycolic acid.
30060403	7	62	theme	acid	1077:1080	arg1	pretreatments					1082:1094	ChCl-formic acid pretreatments	1065:1094	ChCl-formic acid pretreatments of Akebia' herbal residues	1065:1121	A 98.0% level of subsequent enzymatic saccharification and a 100% ethanol yield were achieved after ChCl-formic acid pretreatments of Akebia' herbal residues.
30060403	3	63	theme	solid	404:408	arg1	loss					410:413	solid loss	404:413	solid loss	404:413	Reaction temperatures had more significant influences on solid loss and chemical composition than the molar ratios of choline chloride (ChCl) to derived acids.
26256190	5	0	theme	sulfated	674:681	arg1	xylomannans					683:693	the sulfated xylomannans	670:693	the sulfated xylomannans S1F1 and S2F1	670:707	Especially, the sulfated xylomannans S1F1 and S2F1 possessed altered activity against HSV-1 and HSV-2 compared to the parental compound (F1) and that too in the absence of drug-induced cytotoxicity.
26256190	5	0	theme	sulfated	674:681	arg1	S2F1					704:707	S2F1	704:707	S2F1	704:707	Especially, the sulfated xylomannans S1F1 and S2F1 possessed altered activity against HSV-1 and HSV-2 compared to the parental compound (F1) and that too in the absence of drug-induced cytotoxicity.
26256190	5	0	theme	sulfated	674:681	arg1	S1F1					695:698	S1F1	695:698	S1F1	695:698	Especially, the sulfated xylomannans S1F1 and S2F1 possessed altered activity against HSV-1 and HSV-2 compared to the parental compound (F1) and that too in the absence of drug-induced cytotoxicity.
26256190	5	1	theme	cytotoxicity	843:854	arg1	absence					819:825	the absence	815:825	the absence of drug-induced cytotoxicity	815:854	Especially, the sulfated xylomannans S1F1 and S2F1 possessed altered activity against HSV-1 and HSV-2 compared to the parental compound (F1) and that too in the absence of drug-induced cytotoxicity.
26256190	1	2	theme	heparan	157:163	arg1	proteoglycans					173:185	cell-surface heparan sulfate proteoglycans	144:185	cell-surface heparan sulfate proteoglycans with biological relevance in virus entry	144:226	Herpes simplex viruses (HSVs) display affinity for cell-surface heparan sulfate proteoglycans with biological relevance in virus entry.
26256190	2	3	from	hatei	326:330	arg1	xylomannans					301:311	chemically engineered sulfated xylomannans	270:311	chemically engineered sulfated xylomannans from Scinaia hatei	270:330	This study demonstrates the potential of chemically engineered sulfated xylomannans from Scinaia hatei as antiHSV drug candidate.
26256190	2	3	from	hatei	326:330	arg1	potential					257:265	the potential	253:265	the potential of chemically engineered sulfated xylomannans from Scinaia hatei	253:330	This study demonstrates the potential of chemically engineered sulfated xylomannans from Scinaia hatei as antiHSV drug candidate.
26256190	6	4	theme	sulfate	945:951	arg1	group					953:957	sulfate group	945:957	sulfate group	945:957	Regarding methodological facet, the directive decoration of hydroxyl functionality with sulfate group plus changes in the molecular mass and sugar composition during isolation by the used reagent opens a door for the production of new molecular entity with altered biological activity from other natural sources.
26256190	1	5	theme	sulfate	165:171	arg1	proteoglycans					173:185	cell-surface heparan sulfate proteoglycans	144:185	cell-surface heparan sulfate proteoglycans with biological relevance in virus entry	144:226	Herpes simplex viruses (HSVs) display affinity for cell-surface heparan sulfate proteoglycans with biological relevance in virus entry.
26256190	3	6	theme	-SO3/pyridine	393:405	arg1	procedure					413:421	a dimethylformamide -SO3/pyridine based procedure	373:421	a dimethylformamide -SO3/pyridine based procedure	373:421	Particularly, a dimethylformamide -SO3/pyridine based procedure has been employed for the generation of anionic polysaccharides.
26256190	4	7	theme	xylomannans	553:563	arg1	spectrum					541:548	a spectrum	539:548	a spectrum of xylomannans	539:563	This one-step procedure has the power of providing a spectrum of xylomannans with varying molecular masses (<12-74kDa), sulfate content (1-50%) and glycosyl composition.
26256190	2	8	theme	Scinaia	318:324	arg1	hatei					326:330	Scinaia hatei	318:330	Scinaia hatei	318:330	This study demonstrates the potential of chemically engineered sulfated xylomannans from Scinaia hatei as antiHSV drug candidate.
26256190	2	9	theme	drug	343:346	arg1	candidate					348:356	antiHSV drug candidate	335:356	antiHSV drug candidate	335:356	This study demonstrates the potential of chemically engineered sulfated xylomannans from Scinaia hatei as antiHSV drug candidate.
26256190	6	10	theme	functionality	926:938	arg1	decoration					903:912	the directive decoration	889:912	the directive decoration of hydroxyl functionality with sulfate group plus changes in the molecular mass and sugar composition during isolation by the used reagent	889:1051	Regarding methodological facet, the directive decoration of hydroxyl functionality with sulfate group plus changes in the molecular mass and sugar composition during isolation by the used reagent opens a door for the production of new molecular entity with altered biological activity from other natural sources.
26256190	4	11	theme	one-step	493:500	arg1	procedure					502:510	This one-step procedure	488:510	This one-step procedure	488:510	This one-step procedure has the power of providing a spectrum of xylomannans with varying molecular masses (<12-74kDa), sulfate content (1-50%) and glycosyl composition.
26256190	6	12	theme	used	1040:1043	arg1	reagent					1045:1051	the used reagent	1036:1051	the used reagent	1036:1051	Regarding methodological facet, the directive decoration of hydroxyl functionality with sulfate group plus changes in the molecular mass and sugar composition during isolation by the used reagent opens a door for the production of new molecular entity with altered biological activity from other natural sources.
26256190	0	13	from	hatei	55:59	arg1	sulfates					33:40	xylomannan sulfates	22:40	xylomannan sulfates from Scinaia hatei and their antiviral activities	22:90	Additionally sulfated xylomannan sulfates from Scinaia hatei and their antiviral activities.
26256190	6	14	theme	altered	1114:1120	arg1	activity					1133:1140	altered biological activity	1114:1140	altered biological activity from other natural sources	1114:1167	Regarding methodological facet, the directive decoration of hydroxyl functionality with sulfate group plus changes in the molecular mass and sugar composition during isolation by the used reagent opens a door for the production of new molecular entity with altered biological activity from other natural sources.
26256190	2	15	theme	xylomannans	301:311	arg1	potential					257:265	the potential	253:265	the potential of chemically engineered sulfated xylomannans from Scinaia hatei	253:330	This study demonstrates the potential of chemically engineered sulfated xylomannans from Scinaia hatei as antiHSV drug candidate.
26256190	3	16	theme	anionic	463:469	arg1	polysaccharides					471:485	anionic polysaccharides	463:485	anionic polysaccharides	463:485	Particularly, a dimethylformamide -SO3/pyridine based procedure has been employed for the generation of anionic polysaccharides.
26256190	6	17	theme	entity	1102:1107	arg1	production					1074:1083	the production	1070:1083	the production of new molecular entity with altered biological activity from other natural sources	1070:1167	Regarding methodological facet, the directive decoration of hydroxyl functionality with sulfate group plus changes in the molecular mass and sugar composition during isolation by the used reagent opens a door for the production of new molecular entity with altered biological activity from other natural sources.
26256190	3	18	theme	dimethylformamide	375:391	arg1	procedure					413:421	a dimethylformamide -SO3/pyridine based procedure	373:421	a dimethylformamide -SO3/pyridine based procedure	373:421	Particularly, a dimethylformamide -SO3/pyridine based procedure has been employed for the generation of anionic polysaccharides.
26256190	6	19	with	decoration	903:912	arg1	changes					964:970	changes	964:970	changes in the molecular mass and sugar composition during isolation by the used reagent	964:1051	Regarding methodological facet, the directive decoration of hydroxyl functionality with sulfate group plus changes in the molecular mass and sugar composition during isolation by the used reagent opens a door for the production of new molecular entity with altered biological activity from other natural sources.
26256190	6	19	with	decoration	903:912	arg1	group					953:957	sulfate group	945:957	sulfate group	945:957	Regarding methodological facet, the directive decoration of hydroxyl functionality with sulfate group plus changes in the molecular mass and sugar composition during isolation by the used reagent opens a door for the production of new molecular entity with altered biological activity from other natural sources.
26256190	2	20	theme	sulfated	292:299	arg1	xylomannans					301:311	chemically engineered sulfated xylomannans	270:311	chemically engineered sulfated xylomannans from Scinaia hatei	270:330	This study demonstrates the potential of chemically engineered sulfated xylomannans from Scinaia hatei as antiHSV drug candidate.
26256190	6	21	theme	molecular	1092:1100	arg1	entity					1102:1107	new molecular entity	1088:1107	new molecular entity with altered biological activity from other natural sources	1088:1167	Regarding methodological facet, the directive decoration of hydroxyl functionality with sulfate group plus changes in the molecular mass and sugar composition during isolation by the used reagent opens a door for the production of new molecular entity with altered biological activity from other natural sources.
26256190	5	22	theme	compound	785:792	arg1	F1					795:796	the parental compound (F1)	772:797	the parental compound (F1)	772:797	Especially, the sulfated xylomannans S1F1 and S2F1 possessed altered activity against HSV-1 and HSV-2 compared to the parental compound (F1) and that too in the absence of drug-induced cytotoxicity.
26256190	1	23	theme	Herpes	93:98	arg1	HSVs					117:120	HSVs	117:120	HSVs	117:120	Herpes simplex viruses (HSVs) display affinity for cell-surface heparan sulfate proteoglycans with biological relevance in virus entry.
26256190	1	23	theme	Herpes	93:98	arg1	viruses					108:114	Herpes simplex viruses	93:114	Herpes simplex viruses (HSVs)	93:121	Herpes simplex viruses (HSVs) display affinity for cell-surface heparan sulfate proteoglycans with biological relevance in virus entry.
26256190	6	24	theme	hydroxyl	917:924	arg1	functionality					926:938	hydroxyl functionality	917:938	hydroxyl functionality	917:938	Regarding methodological facet, the directive decoration of hydroxyl functionality with sulfate group plus changes in the molecular mass and sugar composition during isolation by the used reagent opens a door for the production of new molecular entity with altered biological activity from other natural sources.
26256190	1	25	theme	biological	192:201	arg1	relevance					203:211	biological relevance	192:211	biological relevance in virus entry	192:226	Herpes simplex viruses (HSVs) display affinity for cell-surface heparan sulfate proteoglycans with biological relevance in virus entry.
26256190	2	26	theme	antiHSV	335:341	arg1	candidate					348:356	antiHSV drug candidate	335:356	antiHSV drug candidate	335:356	This study demonstrates the potential of chemically engineered sulfated xylomannans from Scinaia hatei as antiHSV drug candidate.
26256190	3	27	theme	polysaccharides	471:485	arg1	generation					449:458	the generation	445:458	the generation of anionic polysaccharides	445:485	Particularly, a dimethylformamide -SO3/pyridine based procedure has been employed for the generation of anionic polysaccharides.
26256190	6	28	theme	sugar	998:1002	arg1	composition					1004:1014	sugar composition	998:1014	sugar composition	998:1014	Regarding methodological facet, the directive decoration of hydroxyl functionality with sulfate group plus changes in the molecular mass and sugar composition during isolation by the used reagent opens a door for the production of new molecular entity with altered biological activity from other natural sources.
26256190	2	29	from	potential	257:265	arg1	hatei					326:330	Scinaia hatei	318:330	Scinaia hatei	318:330	This study demonstrates the potential of chemically engineered sulfated xylomannans from Scinaia hatei as antiHSV drug candidate.
26256190	4	30	contain	has	512:514	arg1	procedure					502:510	This one-step procedure	488:510	This one-step procedure	488:510	This one-step procedure has the power of providing a spectrum of xylomannans with varying molecular masses (<12-74kDa), sulfate content (1-50%) and glycosyl composition.
26256190	4	30	contain	has	512:514	arg2	power					520:524	the power	516:524	the power of providing a spectrum of xylomannans with varying molecular masses (<12-74kDa), sulfate content (1-50%) and glycosyl composition	516:655	This one-step procedure has the power of providing a spectrum of xylomannans with varying molecular masses (<12-74kDa), sulfate content (1-50%) and glycosyl composition.
26256190	1	31	theme	simplex	100:106	arg1	HSVs					117:120	HSVs	117:120	HSVs	117:120	Herpes simplex viruses (HSVs) display affinity for cell-surface heparan sulfate proteoglycans with biological relevance in virus entry.
26256190	1	31	theme	simplex	100:106	arg1	viruses					108:114	Herpes simplex viruses	93:114	Herpes simplex viruses (HSVs)	93:121	Herpes simplex viruses (HSVs) display affinity for cell-surface heparan sulfate proteoglycans with biological relevance in virus entry.
26256190	4	32	theme	sulfate	608:614	arg1	content					616:622	sulfate content	608:622	sulfate content (1-50%)	608:630	This one-step procedure has the power of providing a spectrum of xylomannans with varying molecular masses (<12-74kDa), sulfate content (1-50%) and glycosyl composition.
26256190	4	32	theme	sulfate	608:614	arg1	%					629:629	1-50%	625:629	1-50%	625:629	This one-step procedure has the power of providing a spectrum of xylomannans with varying molecular masses (<12-74kDa), sulfate content (1-50%) and glycosyl composition.
26256190	6	33	theme	directive	893:901	arg1	decoration					903:912	the directive decoration	889:912	the directive decoration of hydroxyl functionality with sulfate group plus changes in the molecular mass and sugar composition during isolation by the used reagent	889:1051	Regarding methodological facet, the directive decoration of hydroxyl functionality with sulfate group plus changes in the molecular mass and sugar composition during isolation by the used reagent opens a door for the production of new molecular entity with altered biological activity from other natural sources.
26256190	1	34	theme	virus	216:220	arg1	entry					222:226	virus entry	216:226	virus entry	216:226	Herpes simplex viruses (HSVs) display affinity for cell-surface heparan sulfate proteoglycans with biological relevance in virus entry.
26256190	0	35	from	activities	81:90	arg1	sulfates					33:40	xylomannan sulfates	22:40	xylomannan sulfates from Scinaia hatei and their antiviral activities	22:90	Additionally sulfated xylomannan sulfates from Scinaia hatei and their antiviral activities.
26256190	6	36	theme	new	1088:1090	arg1	entity					1102:1107	new molecular entity	1088:1107	new molecular entity with altered biological activity from other natural sources	1088:1167	Regarding methodological facet, the directive decoration of hydroxyl functionality with sulfate group plus changes in the molecular mass and sugar composition during isolation by the used reagent opens a door for the production of new molecular entity with altered biological activity from other natural sources.
26256190	4	37	theme	molecular	578:586	arg1	masses					588:593	molecular masses	578:593	molecular masses (<12-74kDa)	578:605	This one-step procedure has the power of providing a spectrum of xylomannans with varying molecular masses (<12-74kDa), sulfate content (1-50%) and glycosyl composition.
26256190	4	37	theme	molecular	578:586	arg1	<12-74kDa					596:604	<12-74kDa	596:604	<12-74kDa	596:604	This one-step procedure has the power of providing a spectrum of xylomannans with varying molecular masses (<12-74kDa), sulfate content (1-50%) and glycosyl composition.
26256190	6	38	with	entity	1102:1107	arg1	activity					1133:1140	altered biological activity	1114:1140	altered biological activity from other natural sources	1114:1167	Regarding methodological facet, the directive decoration of hydroxyl functionality with sulfate group plus changes in the molecular mass and sugar composition during isolation by the used reagent opens a door for the production of new molecular entity with altered biological activity from other natural sources.
26256190	5	39	theme	parental	776:783	arg1	F1					795:796	the parental compound (F1)	772:797	the parental compound (F1)	772:797	Especially, the sulfated xylomannans S1F1 and S2F1 possessed altered activity against HSV-1 and HSV-2 compared to the parental compound (F1) and that too in the absence of drug-induced cytotoxicity.
26256190	6	40	theme	molecular	979:987	arg1	mass					989:992	the molecular mass	975:992	the molecular mass	975:992	Regarding methodological facet, the directive decoration of hydroxyl functionality with sulfate group plus changes in the molecular mass and sugar composition during isolation by the used reagent opens a door for the production of new molecular entity with altered biological activity from other natural sources.
26256190	4	41	theme	glycosyl	636:643	arg1	composition					645:655	glycosyl composition	636:655	glycosyl composition	636:655	This one-step procedure has the power of providing a spectrum of xylomannans with varying molecular masses (<12-74kDa), sulfate content (1-50%) and glycosyl composition.
26256190	5	42	contain	possessed	709:717	arg1	xylomannans					683:693	the sulfated xylomannans	670:693	the sulfated xylomannans S1F1 and S2F1	670:707	Especially, the sulfated xylomannans S1F1 and S2F1 possessed altered activity against HSV-1 and HSV-2 compared to the parental compound (F1) and that too in the absence of drug-induced cytotoxicity.
26256190	5	42	contain	possessed	709:717	arg1	S2F1					704:707	S2F1	704:707	S2F1	704:707	Especially, the sulfated xylomannans S1F1 and S2F1 possessed altered activity against HSV-1 and HSV-2 compared to the parental compound (F1) and that too in the absence of drug-induced cytotoxicity.
26256190	5	42	contain	possessed	709:717	arg1	S1F1					695:698	S1F1	695:698	S1F1	695:698	Especially, the sulfated xylomannans S1F1 and S2F1 possessed altered activity against HSV-1 and HSV-2 compared to the parental compound (F1) and that too in the absence of drug-induced cytotoxicity.
26256190	5	42	contain	possessed	709:717	arg2	activity					727:734	altered activity	719:734	altered activity against HSV-1 and HSV-2	719:758	Especially, the sulfated xylomannans S1F1 and S2F1 possessed altered activity against HSV-1 and HSV-2 compared to the parental compound (F1) and that too in the absence of drug-induced cytotoxicity.
26256190	6	43	from	changes	964:970	arg1	mass					989:992	the molecular mass	975:992	the molecular mass	975:992	Regarding methodological facet, the directive decoration of hydroxyl functionality with sulfate group plus changes in the molecular mass and sugar composition during isolation by the used reagent opens a door for the production of new molecular entity with altered biological activity from other natural sources.
26256190	6	43	from	changes	964:970	arg1	composition					1004:1014	sugar composition	998:1014	sugar composition	998:1014	Regarding methodological facet, the directive decoration of hydroxyl functionality with sulfate group plus changes in the molecular mass and sugar composition during isolation by the used reagent opens a door for the production of new molecular entity with altered biological activity from other natural sources.
26256190	6	44	theme	methodological	867:880	arg1	facet					882:886	methodological facet	867:886	methodological facet	867:886	Regarding methodological facet, the directive decoration of hydroxyl functionality with sulfate group plus changes in the molecular mass and sugar composition during isolation by the used reagent opens a door for the production of new molecular entity with altered biological activity from other natural sources.
26256190	6	45	from	sources	1161:1167	arg1	activity					1133:1140	altered biological activity	1114:1140	altered biological activity from other natural sources	1114:1167	Regarding methodological facet, the directive decoration of hydroxyl functionality with sulfate group plus changes in the molecular mass and sugar composition during isolation by the used reagent opens a door for the production of new molecular entity with altered biological activity from other natural sources.
26256190	6	46	theme	natural	1153:1159	arg1	sources					1161:1167	other natural sources	1147:1167	other natural sources	1147:1167	Regarding methodological facet, the directive decoration of hydroxyl functionality with sulfate group plus changes in the molecular mass and sugar composition during isolation by the used reagent opens a door for the production of new molecular entity with altered biological activity from other natural sources.
26256190	1	47	with	proteoglycans	173:185	arg1	relevance					203:211	biological relevance	192:211	biological relevance in virus entry	192:226	Herpes simplex viruses (HSVs) display affinity for cell-surface heparan sulfate proteoglycans with biological relevance in virus entry.
26256190	2	48	theme	engineered	281:290	arg1	xylomannans					301:311	chemically engineered sulfated xylomannans	270:311	chemically engineered sulfated xylomannans from Scinaia hatei	270:330	This study demonstrates the potential of chemically engineered sulfated xylomannans from Scinaia hatei as antiHSV drug candidate.
26256190	6	49	theme	other	1147:1151	arg1	sources					1161:1167	other natural sources	1147:1167	other natural sources	1147:1167	Regarding methodological facet, the directive decoration of hydroxyl functionality with sulfate group plus changes in the molecular mass and sugar composition during isolation by the used reagent opens a door for the production of new molecular entity with altered biological activity from other natural sources.
26256190	5	50	theme	drug-induced	830:841	arg1	cytotoxicity					843:854	drug-induced cytotoxicity	830:854	drug-induced cytotoxicity	830:854	Especially, the sulfated xylomannans S1F1 and S2F1 possessed altered activity against HSV-1 and HSV-2 compared to the parental compound (F1) and that too in the absence of drug-induced cytotoxicity.
26256190	6	51	theme	biological	1122:1131	arg1	activity					1133:1140	altered biological activity	1114:1140	altered biological activity from other natural sources	1114:1167	Regarding methodological facet, the directive decoration of hydroxyl functionality with sulfate group plus changes in the molecular mass and sugar composition during isolation by the used reagent opens a door for the production of new molecular entity with altered biological activity from other natural sources.
26256190	0	52	theme	antiviral	71:79	arg1	activities					81:90	their antiviral activities	65:90	their antiviral activities	65:90	Additionally sulfated xylomannan sulfates from Scinaia hatei and their antiviral activities.
26256190	1	53	from	relevance	203:211	arg1	entry					222:226	virus entry	216:226	virus entry	216:226	Herpes simplex viruses (HSVs) display affinity for cell-surface heparan sulfate proteoglycans with biological relevance in virus entry.
26256190	5	54	theme	altered	719:725	arg1	activity					727:734	altered activity	719:734	altered activity against HSV-1 and HSV-2	719:758	Especially, the sulfated xylomannans S1F1 and S2F1 possessed altered activity against HSV-1 and HSV-2 compared to the parental compound (F1) and that too in the absence of drug-induced cytotoxicity.
26256190	5	55	dep	xylomannans	683:693	arg1	xylomannans					683:693	the sulfated xylomannans	670:693	the sulfated xylomannans S1F1 and S2F1	670:707	Especially, the sulfated xylomannans S1F1 and S2F1 possessed altered activity against HSV-1 and HSV-2 compared to the parental compound (F1) and that too in the absence of drug-induced cytotoxicity.
26256190	5	55	dep	xylomannans	683:693	arg1	S2F1					704:707	S2F1	704:707	S2F1	704:707	Especially, the sulfated xylomannans S1F1 and S2F1 possessed altered activity against HSV-1 and HSV-2 compared to the parental compound (F1) and that too in the absence of drug-induced cytotoxicity.
26256190	5	55	dep	xylomannans	683:693	arg1	S1F1					695:698	S1F1	695:698	S1F1	695:698	Especially, the sulfated xylomannans S1F1 and S2F1 possessed altered activity against HSV-1 and HSV-2 compared to the parental compound (F1) and that too in the absence of drug-induced cytotoxicity.
26256190	0	56	theme	xylomannan	22:31	arg1	sulfates					33:40	xylomannan sulfates	22:40	xylomannan sulfates from Scinaia hatei and their antiviral activities	22:90	Additionally sulfated xylomannan sulfates from Scinaia hatei and their antiviral activities.
26256190	1	57	theme	cell-surface	144:155	arg1	proteoglycans					173:185	cell-surface heparan sulfate proteoglycans	144:185	cell-surface heparan sulfate proteoglycans with biological relevance in virus entry	144:226	Herpes simplex viruses (HSVs) display affinity for cell-surface heparan sulfate proteoglycans with biological relevance in virus entry.
26256190	3	58	theme	based	407:411	arg1	procedure					413:421	a dimethylformamide -SO3/pyridine based procedure	373:421	a dimethylformamide -SO3/pyridine based procedure	373:421	Particularly, a dimethylformamide -SO3/pyridine based procedure has been employed for the generation of anionic polysaccharides.
24283890	4	0	from	level	571:575	arg1	composition					607:617	the composition	603:617	the composition	603:617	The type and level of hydrophilic carrier in the composition affected the dissolution profiles of the extrudates.
24283890	1	1	theme	film	327:330	arg1	technology					347:356	thin film freezing (TFF) technology	322:356	thin film freezing (TFF) technology	322:356	We investigated the effects of the hot-melt extrusion (HME) process on the properties of itraconazole (ITZ) amorphous solid dispersions made by thin film freezing (TFF) technology.
24283890	3	2	theme	TFF	468:470	arg1	composition					472:482	the ITZ-HPMCAS TFF composition	453:482	the ITZ-HPMCAS TFF composition	453:482	HME of the ITZ-HPMCAS TFF composition with hydrophilic carriers improved the drug release rate in acidic media.
24283890	7	3	from	release	1023:1029	arg1	media					1045:1049	the acidic media	1034:1049	the acidic media	1034:1049	A higher degree of mixing reduced the crystallinity of semicrystalline excipients and favored the drug release in the acidic media; moreover, the drug precipitation rate in the neutral pH media was reduced.
24283890	1	4	theme	freezing	332:339	arg1	technology					347:356	thin film freezing (TFF) technology	322:356	thin film freezing (TFF) technology	322:356	We investigated the effects of the hot-melt extrusion (HME) process on the properties of itraconazole (ITZ) amorphous solid dispersions made by thin film freezing (TFF) technology.
24283890	4	5	theme	carrier	592:598	arg1	level					571:575	level	571:575	level	571:575	The type and level of hydrophilic carrier in the composition affected the dissolution profiles of the extrudates.
24283890	4	5	theme	carrier	592:598	arg1	type					562:565	type	562:565	type	562:565	The type and level of hydrophilic carrier in the composition affected the dissolution profiles of the extrudates.
24283890	3	6	theme	composition	472:482	arg1	HME					446:448	HME	446:448	HME of the ITZ-HPMCAS TFF composition with hydrophilic carriers	446:508	HME of the ITZ-HPMCAS TFF composition with hydrophilic carriers improved the drug release rate in acidic media.
24283890	0	7	theme	extrusion	100:108	arg1	combination					76:86	the combination	72:86	the combination of hot-melt extrusion	72:108	Dissolution enhancement of itraconazole by hot-melt extrusion alone and the combination of hot-melt extrusion and rapid freezing--effect of formulation and processing variables.
24283890	0	7	theme	extrusion	100:108	arg1	freezing--effect					120:135	rapid freezing--effect	114:135	rapid freezing--effect of formulation and processing variables	114:175	Dissolution enhancement of itraconazole by hot-melt extrusion alone and the combination of hot-melt extrusion and rapid freezing--effect of formulation and processing variables.
24283890	7	8	theme	precipitation	1071:1083	arg1	rate					1085:1088	the drug precipitation rate	1062:1088	the drug precipitation rate in the neutral pH media	1062:1112	A higher degree of mixing reduced the crystallinity of semicrystalline excipients and favored the drug release in the acidic media; moreover, the drug precipitation rate in the neutral pH media was reduced.
24283890	7	9	theme	mixing	939:944	arg1	degree					929:934	A higher degree	920:934	A higher degree of mixing	920:944	A higher degree of mixing reduced the crystallinity of semicrystalline excipients and favored the drug release in the acidic media; moreover, the drug precipitation rate in the neutral pH media was reduced.
24283890	0	10	theme	hot-melt	91:98	arg1	extrusion					100:108	hot-melt extrusion	91:108	hot-melt extrusion	91:108	Dissolution enhancement of itraconazole by hot-melt extrusion alone and the combination of hot-melt extrusion and rapid freezing--effect of formulation and processing variables.
24283890	1	11	theme	TFF	342:344	arg1	technology					347:356	thin film freezing (TFF) technology	322:356	thin film freezing (TFF) technology	322:356	We investigated the effects of the hot-melt extrusion (HME) process on the properties of itraconazole (ITZ) amorphous solid dispersions made by thin film freezing (TFF) technology.
24283890	4	12	from	type	562:565	arg1	composition					607:617	the composition	603:617	the composition	603:617	The type and level of hydrophilic carrier in the composition affected the dissolution profiles of the extrudates.
24283890	6	13	theme	processing	769:778	arg1	temperature					808:818	extrusion temperature	798:818	extrusion temperature	798:818	Hot-melt extrusion processing variables such as extrusion temperature and screw configuration also played a critical role on the properties of the extruded compositions.
24283890	6	13	theme	processing	769:778	arg1	configuration					830:842	screw configuration	824:842	screw configuration	824:842	Hot-melt extrusion processing variables such as extrusion temperature and screw configuration also played a critical role on the properties of the extruded compositions.
24283890	6	13	theme	processing	769:778	arg1	variables					780:788	Hot-melt extrusion processing variables	750:788	Hot-melt extrusion processing variables such as extrusion temperature and screw configuration	750:842	Hot-melt extrusion processing variables such as extrusion temperature and screw configuration also played a critical role on the properties of the extruded compositions.
24283890	3	14	theme	drug	523:526	arg1	rate					536:539	the drug release rate	519:539	the drug release rate	519:539	HME of the ITZ-HPMCAS TFF composition with hydrophilic carriers improved the drug release rate in acidic media.
24283890	0	15	theme	rapid	114:118	arg1	freezing--effect					120:135	rapid freezing--effect	114:135	rapid freezing--effect of formulation and processing variables	114:175	Dissolution enhancement of itraconazole by hot-melt extrusion alone and the combination of hot-melt extrusion and rapid freezing--effect of formulation and processing variables.
24283890	3	16	with	HME	446:448	arg1	carriers					501:508	hydrophilic carriers	489:508	hydrophilic carriers	489:508	HME of the ITZ-HPMCAS TFF composition with hydrophilic carriers improved the drug release rate in acidic media.
24283890	2	17	theme	TFF	382:384	arg1	composition					386:396	The ITZ-HPMCAS L (1:2) TFF composition	359:396	The ITZ-HPMCAS L (1:2) TFF composition	359:396	The ITZ-HPMCAS L (1:2) TFF composition exhibited limited drug release in acidic media.
24283890	7	18	theme	pH	1105:1106	arg1	media					1108:1112	the neutral pH media	1093:1112	the neutral pH media	1093:1112	A higher degree of mixing reduced the crystallinity of semicrystalline excipients and favored the drug release in the acidic media; moreover, the drug precipitation rate in the neutral pH media was reduced.
24283890	6	19	theme	critical	858:865	arg1	role					867:870	a critical role	856:870	a critical role	856:870	Hot-melt extrusion processing variables such as extrusion temperature and screw configuration also played a critical role on the properties of the extruded compositions.
24283890	7	20	theme	drug	1066:1069	arg1	rate					1085:1088	the drug precipitation rate	1062:1088	the drug precipitation rate in the neutral pH media	1062:1112	A higher degree of mixing reduced the crystallinity of semicrystalline excipients and favored the drug release in the acidic media; moreover, the drug precipitation rate in the neutral pH media was reduced.
24283890	6	21	theme	Hot-melt	750:757	arg1	temperature					808:818	extrusion temperature	798:818	extrusion temperature	798:818	Hot-melt extrusion processing variables such as extrusion temperature and screw configuration also played a critical role on the properties of the extruded compositions.
24283890	6	21	theme	Hot-melt	750:757	arg1	configuration					830:842	screw configuration	824:842	screw configuration	824:842	Hot-melt extrusion processing variables such as extrusion temperature and screw configuration also played a critical role on the properties of the extruded compositions.
24283890	6	21	theme	Hot-melt	750:757	arg1	variables					780:788	Hot-melt extrusion processing variables	750:788	Hot-melt extrusion processing variables such as extrusion temperature and screw configuration	750:842	Hot-melt extrusion processing variables such as extrusion temperature and screw configuration also played a critical role on the properties of the extruded compositions.
24283890	0	22	theme	Dissolution	0:10	arg1	enhancement					12:22	Dissolution enhancement	0:22	Dissolution enhancement of itraconazole by hot-melt extrusion	0:60	Dissolution enhancement of itraconazole by hot-melt extrusion alone and the combination of hot-melt extrusion and rapid freezing--effect of formulation and processing variables.
24283890	2	23	theme	L	374:374	arg1	composition					386:396	The ITZ-HPMCAS L (1:2) TFF composition	359:396	The ITZ-HPMCAS L (1:2) TFF composition	359:396	The ITZ-HPMCAS L (1:2) TFF composition exhibited limited drug release in acidic media.
24283890	7	24	theme	higher	922:927	arg1	degree					929:934	A higher degree	920:934	A higher degree of mixing	920:944	A higher degree of mixing reduced the crystallinity of semicrystalline excipients and favored the drug release in the acidic media; moreover, the drug precipitation rate in the neutral pH media was reduced.
24283890	2	25	theme	ITZ-HPMCAS	363:372	arg1	composition					386:396	The ITZ-HPMCAS L (1:2) TFF composition	359:396	The ITZ-HPMCAS L (1:2) TFF composition	359:396	The ITZ-HPMCAS L (1:2) TFF composition exhibited limited drug release in acidic media.
24283890	2	26	theme	drug	416:419	arg1	release					421:427	limited drug release	408:427	limited drug release	408:427	The ITZ-HPMCAS L (1:2) TFF composition exhibited limited drug release in acidic media.
24283890	3	27	theme	acidic	544:549	arg1	media					551:555	acidic media	544:555	acidic media	544:555	HME of the ITZ-HPMCAS TFF composition with hydrophilic carriers improved the drug release rate in acidic media.
24283890	4	28	from	composition	607:617	arg1	level					571:575	level	571:575	level	571:575	The type and level of hydrophilic carrier in the composition affected the dissolution profiles of the extrudates.
24283890	4	28	from	composition	607:617	arg1	type					562:565	type	562:565	type	562:565	The type and level of hydrophilic carrier in the composition affected the dissolution profiles of the extrudates.
24283890	4	29	theme	dissolution	632:642	arg1	profiles					644:651	the dissolution profiles	628:651	the dissolution profiles of the extrudates	628:669	The type and level of hydrophilic carrier in the composition affected the dissolution profiles of the extrudates.
24283890	0	30	theme	itraconazole	27:38	arg1	enhancement					12:22	Dissolution enhancement	0:22	Dissolution enhancement of itraconazole by hot-melt extrusion	0:60	Dissolution enhancement of itraconazole by hot-melt extrusion alone and the combination of hot-melt extrusion and rapid freezing--effect of formulation and processing variables.
24283890	6	31	theme	extrusion	759:767	arg1	temperature					808:818	extrusion temperature	798:818	extrusion temperature	798:818	Hot-melt extrusion processing variables such as extrusion temperature and screw configuration also played a critical role on the properties of the extruded compositions.
24283890	6	31	theme	extrusion	759:767	arg1	configuration					830:842	screw configuration	824:842	screw configuration	824:842	Hot-melt extrusion processing variables such as extrusion temperature and screw configuration also played a critical role on the properties of the extruded compositions.
24283890	6	31	theme	extrusion	759:767	arg1	variables					780:788	Hot-melt extrusion processing variables	750:788	Hot-melt extrusion processing variables such as extrusion temperature and screw configuration	750:842	Hot-melt extrusion processing variables such as extrusion temperature and screw configuration also played a critical role on the properties of the extruded compositions.
24283890	3	32	theme	release	528:534	arg1	rate					536:539	the drug release rate	519:539	the drug release rate	519:539	HME of the ITZ-HPMCAS TFF composition with hydrophilic carriers improved the drug release rate in acidic media.
24283890	5	33	theme	design	674:679	arg1	study					702:706	A design of experiments (DoE) study	672:706	A design of experiments (DoE) study	672:706	A design of experiments (DoE) study was conducted to elucidate those effects.
24283890	4	34	dep	type	562:565	arg1	The					558:560	The	558:560	The	558:560	The type and level of hydrophilic carrier in the composition affected the dissolution profiles of the extrudates.
24283890	4	35	theme	extrudates	660:669	arg1	profiles					644:651	the dissolution profiles	628:651	the dissolution profiles of the extrudates	628:669	The type and level of hydrophilic carrier in the composition affected the dissolution profiles of the extrudates.
24283890	1	36	theme	hot-melt	213:220	arg1	process					238:244	the hot-melt extrusion (HME) process	209:244	the hot-melt extrusion (HME) process	209:244	We investigated the effects of the hot-melt extrusion (HME) process on the properties of itraconazole (ITZ) amorphous solid dispersions made by thin film freezing (TFF) technology.
24283890	0	37	theme	hot-melt	43:50	arg1	extrusion					52:60	hot-melt extrusion	43:60	hot-melt extrusion	43:60	Dissolution enhancement of itraconazole by hot-melt extrusion alone and the combination of hot-melt extrusion and rapid freezing--effect of formulation and processing variables.
24283890	1	38	theme	amorphous	286:294	arg1	dispersions					302:312	itraconazole (ITZ) amorphous solid dispersions	267:312	itraconazole (ITZ) amorphous solid dispersions made by thin film freezing (TFF) technology	267:356	We investigated the effects of the hot-melt extrusion (HME) process on the properties of itraconazole (ITZ) amorphous solid dispersions made by thin film freezing (TFF) technology.
24283890	4	39	from	carrier	592:598	arg1	composition					607:617	the composition	603:617	the composition	603:617	The type and level of hydrophilic carrier in the composition affected the dissolution profiles of the extrudates.
24283890	1	40	theme	extrusion	222:230	arg1	process					238:244	the hot-melt extrusion (HME) process	209:244	the hot-melt extrusion (HME) process	209:244	We investigated the effects of the hot-melt extrusion (HME) process on the properties of itraconazole (ITZ) amorphous solid dispersions made by thin film freezing (TFF) technology.
24283890	1	41	theme	solid	296:300	arg1	dispersions					302:312	itraconazole (ITZ) amorphous solid dispersions	267:312	itraconazole (ITZ) amorphous solid dispersions made by thin film freezing (TFF) technology	267:356	We investigated the effects of the hot-melt extrusion (HME) process on the properties of itraconazole (ITZ) amorphous solid dispersions made by thin film freezing (TFF) technology.
24283890	0	42	theme	formulation	140:150	arg1	variables					167:175	formulation and processing variables	140:175	variables	167:175	Dissolution enhancement of itraconazole by hot-melt extrusion alone and the combination of hot-melt extrusion and rapid freezing--effect of formulation and processing variables.
24283890	2	43	theme	limited	408:414	arg1	release					421:427	limited drug release	408:427	limited drug release	408:427	The ITZ-HPMCAS L (1:2) TFF composition exhibited limited drug release in acidic media.
24283890	2	44	theme	1:2	377:379	arg1	composition					386:396	The ITZ-HPMCAS L (1:2) TFF composition	359:396	The ITZ-HPMCAS L (1:2) TFF composition	359:396	The ITZ-HPMCAS L (1:2) TFF composition exhibited limited drug release in acidic media.
24283890	2	45	theme	acidic	432:437	arg1	media					439:443	acidic media	432:443	acidic media	432:443	The ITZ-HPMCAS L (1:2) TFF composition exhibited limited drug release in acidic media.
24283890	1	46	theme	dispersions	302:312	arg1	properties					253:262	the properties	249:262	the properties of itraconazole (ITZ) amorphous solid dispersions made by thin film freezing (TFF) technology	249:356	We investigated the effects of the hot-melt extrusion (HME) process on the properties of itraconazole (ITZ) amorphous solid dispersions made by thin film freezing (TFF) technology.
24283890	0	47	theme	variables	167:175	arg1	combination					76:86	the combination	72:86	the combination of hot-melt extrusion	72:108	Dissolution enhancement of itraconazole by hot-melt extrusion alone and the combination of hot-melt extrusion and rapid freezing--effect of formulation and processing variables.
24283890	0	47	theme	variables	167:175	arg1	freezing--effect					120:135	rapid freezing--effect	114:135	rapid freezing--effect of formulation and processing variables	114:175	Dissolution enhancement of itraconazole by hot-melt extrusion alone and the combination of hot-melt extrusion and rapid freezing--effect of formulation and processing variables.
24283890	6	48	theme	screw	824:828	arg1	configuration					830:842	screw configuration	824:842	screw configuration	824:842	Hot-melt extrusion processing variables such as extrusion temperature and screw configuration also played a critical role on the properties of the extruded compositions.
24283890	7	49	theme	acidic	1038:1043	arg1	media					1045:1049	the acidic media	1034:1049	the acidic media	1034:1049	A higher degree of mixing reduced the crystallinity of semicrystalline excipients and favored the drug release in the acidic media; moreover, the drug precipitation rate in the neutral pH media was reduced.
24283890	5	50	theme	experiments	684:694	arg1	design					674:679	A design	672:679	A design of experiments (DoE) study	672:706	A design of experiments (DoE) study was conducted to elucidate those effects.
24283890	5	50	theme	experiments	684:694	arg1	DoE					697:699	DoE	697:699	DoE	697:699	A design of experiments (DoE) study was conducted to elucidate those effects.
24283890	6	51	theme	compositions	906:917	arg1	properties					879:888	the properties	875:888	the properties of the extruded compositions	875:917	Hot-melt extrusion processing variables such as extrusion temperature and screw configuration also played a critical role on the properties of the extruded compositions.
24283890	0	52	theme	processing	156:165	arg1	variables					167:175	formulation and processing variables	140:175	variables	167:175	Dissolution enhancement of itraconazole by hot-melt extrusion alone and the combination of hot-melt extrusion and rapid freezing--effect of formulation and processing variables.
24283890	7	53	theme	semicrystalline	975:989	arg1	excipients					991:1000	semicrystalline excipients	975:1000	semicrystalline excipients	975:1000	A higher degree of mixing reduced the crystallinity of semicrystalline excipients and favored the drug release in the acidic media; moreover, the drug precipitation rate in the neutral pH media was reduced.
24283890	3	54	theme	ITZ-HPMCAS	457:466	arg1	composition					472:482	the ITZ-HPMCAS TFF composition	453:482	the ITZ-HPMCAS TFF composition	453:482	HME of the ITZ-HPMCAS TFF composition with hydrophilic carriers improved the drug release rate in acidic media.
24283890	6	55	theme	extruded	897:904	arg1	compositions					906:917	the extruded compositions	893:917	the extruded compositions	893:917	Hot-melt extrusion processing variables such as extrusion temperature and screw configuration also played a critical role on the properties of the extruded compositions.
24283890	7	56	theme	excipients	991:1000	arg1	crystallinity					958:970	the crystallinity	954:970	the crystallinity of semicrystalline excipients	954:1000	A higher degree of mixing reduced the crystallinity of semicrystalline excipients and favored the drug release in the acidic media; moreover, the drug precipitation rate in the neutral pH media was reduced.
24283890	1	57	theme	HME	233:235	arg1	process					238:244	the hot-melt extrusion (HME) process	209:244	the hot-melt extrusion (HME) process	209:244	We investigated the effects of the hot-melt extrusion (HME) process on the properties of itraconazole (ITZ) amorphous solid dispersions made by thin film freezing (TFF) technology.
24283890	7	58	theme	drug	1018:1021	arg1	release					1023:1029	the drug release	1014:1029	the drug release in the acidic media	1014:1049	A higher degree of mixing reduced the crystallinity of semicrystalline excipients and favored the drug release in the acidic media; moreover, the drug precipitation rate in the neutral pH media was reduced.
24283890	6	59	theme	extrusion	798:806	arg1	temperature					808:818	extrusion temperature	798:818	extrusion temperature	798:818	Hot-melt extrusion processing variables such as extrusion temperature and screw configuration also played a critical role on the properties of the extruded compositions.
24283890	1	60	from	effects	198:204	arg1	properties					253:262	the properties	249:262	the properties of itraconazole (ITZ) amorphous solid dispersions made by thin film freezing (TFF) technology	249:356	We investigated the effects of the hot-melt extrusion (HME) process on the properties of itraconazole (ITZ) amorphous solid dispersions made by thin film freezing (TFF) technology.
24283890	4	61	theme	hydrophilic	580:590	arg1	carrier					592:598	hydrophilic carrier	580:598	hydrophilic carrier in the composition	580:617	The type and level of hydrophilic carrier in the composition affected the dissolution profiles of the extrudates.
24283890	1	62	theme	process	238:244	arg1	effects					198:204	the effects	194:204	the effects of the hot-melt extrusion (HME) process on the properties of itraconazole (ITZ) amorphous solid dispersions made by thin film freezing (TFF) technology	194:356	We investigated the effects of the hot-melt extrusion (HME) process on the properties of itraconazole (ITZ) amorphous solid dispersions made by thin film freezing (TFF) technology.
24283890	7	63	theme	neutral	1097:1103	arg1	media					1108:1112	the neutral pH media	1093:1112	the neutral pH media	1093:1112	A higher degree of mixing reduced the crystallinity of semicrystalline excipients and favored the drug release in the acidic media; moreover, the drug precipitation rate in the neutral pH media was reduced.
24283890	1	64	theme	thin	322:325	arg1	technology					347:356	thin film freezing (TFF) technology	322:356	thin film freezing (TFF) technology	322:356	We investigated the effects of the hot-melt extrusion (HME) process on the properties of itraconazole (ITZ) amorphous solid dispersions made by thin film freezing (TFF) technology.
24283890	3	65	theme	hydrophilic	489:499	arg1	carriers					501:508	hydrophilic carriers	489:508	hydrophilic carriers	489:508	HME of the ITZ-HPMCAS TFF composition with hydrophilic carriers improved the drug release rate in acidic media.
24283890	7	66	from	rate	1085:1088	arg1	media					1108:1112	the neutral pH media	1093:1112	the neutral pH media	1093:1112	A higher degree of mixing reduced the crystallinity of semicrystalline excipients and favored the drug release in the acidic media; moreover, the drug precipitation rate in the neutral pH media was reduced.
27987957	3	0	dep	Meanwhile	364:372	arg1	chemical					375:382	chemical	375:382	chemical	375:382	Meanwhile, chemical structure of xanthan gum produced from glycerol is similar to that of the commercial xanthan through FT-IR and NMR.
27987957	1	1	theme	mutant	143:148	arg1	strain					150:155	a mutant strain	141:155	a mutant strain X. campestris CCTCC M2015714 with glycerol as the sole carbon source	141:224	Xanthan gum was produced by a mutant strain X. campestris CCTCC M2015714 with glycerol as the sole carbon source.
27987957	1	2	with	strain	150:155	arg1	glycerol					191:198	glycerol	191:198	glycerol as the sole carbon source	191:224	Xanthan gum was produced by a mutant strain X. campestris CCTCC M2015714 with glycerol as the sole carbon source.
27987957	0	3	theme	CCTCC	97:101	arg1	M2015714					103:110	CCTCC M2015714	97:110	a mutant strain Xanthomonas campestris CCTCC M2015714	58:110	Characterization of xanthan gum produced from glycerol by a mutant strain Xanthomonas campestris CCTCC M2015714.
27987957	2	4	theme	xanthan	277:283	arg1	gum					285:287	xanthan gum	277:287	xanthan gum produced from glycerol	277:310	The monosaccharide composition and molar ratio of xanthan gum produced from glycerol are glucose: mannose: glucuronic acid=2.0:1.65:1.0.
27987957	4	5	theme	which	683:687	arg1	index					670:674	the consistency index	654:674	the consistency index (K) of which	654:687	Remarkably, the molecular weight of xanthan gum produced using our method (3.0±0.14×106Da) is about half that of the commercial one (5.8±0.25×106Da), and the consistency index (K) of which is less than 1/10 that of the commercial xanthan.
27987957	4	5	theme	which	683:687	arg1	xanthan					730:736	the commercial xanthan	715:736	the commercial xanthan	715:736	Remarkably, the molecular weight of xanthan gum produced using our method (3.0±0.14×106Da) is about half that of the commercial one (5.8±0.25×106Da), and the consistency index (K) of which is less than 1/10 that of the commercial xanthan.
27987957	4	5	theme	which	683:687	arg1	1/10					702:705	1/10	702:705	1/10	702:705	Remarkably, the molecular weight of xanthan gum produced using our method (3.0±0.14×106Da) is about half that of the commercial one (5.8±0.25×106Da), and the consistency index (K) of which is less than 1/10 that of the commercial xanthan.
27987957	4	5	theme	which	683:687	arg1	K					677:677	K	677:677	K	677:677	Remarkably, the molecular weight of xanthan gum produced using our method (3.0±0.14×106Da) is about half that of the commercial one (5.8±0.25×106Da), and the consistency index (K) of which is less than 1/10 that of the commercial xanthan.
27987957	5	6	theme	gum	864:866	arg1	structure/application					831:851	the structure/application	827:851	the structure/application of xanthan gum	827:866	This work paves the way for xanthan production from glycerol and is useful for studying the structure/application of xanthan gum.
27987957	2	7	theme	glucuronic	334:343	arg1	acid=2.0:1.65:1.0					345:361	glucuronic acid=2.0:1.65:1.0	334:361	glucose: mannose: glucuronic acid=2.0:1.65:1.0	316:361	The monosaccharide composition and molar ratio of xanthan gum produced from glycerol are glucose: mannose: glucuronic acid=2.0:1.65:1.0.
27987957	1	8	theme	X.	157:158	arg1	M2015714					177:184	X. campestris CCTCC M2015714	157:184	a mutant strain X. campestris CCTCC M2015714 with glycerol as the sole carbon source	141:224	Xanthan gum was produced by a mutant strain X. campestris CCTCC M2015714 with glycerol as the sole carbon source.
27987957	2	9	theme	molar	262:266	arg1	composition					246:256	monosaccharide composition	231:256	monosaccharide composition	231:256	The monosaccharide composition and molar ratio of xanthan gum produced from glycerol are glucose: mannose: glucuronic acid=2.0:1.65:1.0.
27987957	2	9	theme	molar	262:266	arg1	ratio					268:272	molar ratio	262:272	molar ratio	262:272	The monosaccharide composition and molar ratio of xanthan gum produced from glycerol are glucose: mannose: glucuronic acid=2.0:1.65:1.0.
27987957	2	9	theme	molar	262:266	arg1	glucose					316:322	glucose	316:322	glucose: mannose: glucuronic acid=2.0:1.65:1.0	316:361	The monosaccharide composition and molar ratio of xanthan gum produced from glycerol are glucose: mannose: glucuronic acid=2.0:1.65:1.0.
27987957	1	10	theme	campestris	160:169	arg1	M2015714					177:184	X. campestris CCTCC M2015714	157:184	a mutant strain X. campestris CCTCC M2015714 with glycerol as the sole carbon source	141:224	Xanthan gum was produced by a mutant strain X. campestris CCTCC M2015714 with glycerol as the sole carbon source.
27987957	2	11	dep	glucose	316:322	arg1	mannose					325:331	mannose	325:331	glucose: mannose: glucuronic acid=2.0:1.65:1.0	316:361	The monosaccharide composition and molar ratio of xanthan gum produced from glycerol are glucose: mannose: glucuronic acid=2.0:1.65:1.0.
27987957	2	11	dep	glucose	316:322	arg1	acid=2.0:1.65:1.0					345:361	glucuronic acid=2.0:1.65:1.0	334:361	glucose: mannose: glucuronic acid=2.0:1.65:1.0	316:361	The monosaccharide composition and molar ratio of xanthan gum produced from glycerol are glucose: mannose: glucuronic acid=2.0:1.65:1.0.
27987957	1	12	theme	CCTCC	171:175	arg1	M2015714					177:184	X. campestris CCTCC M2015714	157:184	a mutant strain X. campestris CCTCC M2015714 with glycerol as the sole carbon source	141:224	Xanthan gum was produced by a mutant strain X. campestris CCTCC M2015714 with glycerol as the sole carbon source.
27987957	4	13	theme	xanthan	536:542	arg1	gum					544:546	xanthan gum	536:546	xanthan gum	536:546	Remarkably, the molecular weight of xanthan gum produced using our method (3.0±0.14×106Da) is about half that of the commercial one (5.8±0.25×106Da), and the consistency index (K) of which is less than 1/10 that of the commercial xanthan.
27987957	1	14	dep	strain	150:155	arg1	M2015714					177:184	X. campestris CCTCC M2015714	157:184	a mutant strain X. campestris CCTCC M2015714 with glycerol as the sole carbon source	141:224	Xanthan gum was produced by a mutant strain X. campestris CCTCC M2015714 with glycerol as the sole carbon source.
27987957	2	15	dep	composition	246:256	arg1	The					227:229	The	227:229	The	227:229	The monosaccharide composition and molar ratio of xanthan gum produced from glycerol are glucose: mannose: glucuronic acid=2.0:1.65:1.0.
27987957	0	16	theme	gum	28:30	arg1	Characterization					0:15	Characterization	0:15	Characterization of xanthan gum	0:30	Characterization of xanthan gum produced from glycerol by a mutant strain Xanthomonas campestris CCTCC M2015714.
27987957	4	17	theme	xanthan	730:736	arg1	index					670:674	the consistency index	654:674	the consistency index (K) of which	654:687	Remarkably, the molecular weight of xanthan gum produced using our method (3.0±0.14×106Da) is about half that of the commercial one (5.8±0.25×106Da), and the consistency index (K) of which is less than 1/10 that of the commercial xanthan.
27987957	4	17	theme	xanthan	730:736	arg1	xanthan					730:736	the commercial xanthan	715:736	the commercial xanthan	715:736	Remarkably, the molecular weight of xanthan gum produced using our method (3.0±0.14×106Da) is about half that of the commercial one (5.8±0.25×106Da), and the consistency index (K) of which is less than 1/10 that of the commercial xanthan.
27987957	4	17	theme	xanthan	730:736	arg1	1/10					702:705	1/10	702:705	1/10	702:705	Remarkably, the molecular weight of xanthan gum produced using our method (3.0±0.14×106Da) is about half that of the commercial one (5.8±0.25×106Da), and the consistency index (K) of which is less than 1/10 that of the commercial xanthan.
27987957	0	18	dep	strain	67:72	arg1	M2015714					103:110	CCTCC M2015714	97:110	a mutant strain Xanthomonas campestris CCTCC M2015714	58:110	Characterization of xanthan gum produced from glycerol by a mutant strain Xanthomonas campestris CCTCC M2015714.
27987957	2	19	theme	gum	285:287	arg1	composition					246:256	monosaccharide composition	231:256	monosaccharide composition	231:256	The monosaccharide composition and molar ratio of xanthan gum produced from glycerol are glucose: mannose: glucuronic acid=2.0:1.65:1.0.
27987957	2	19	theme	gum	285:287	arg1	ratio					268:272	molar ratio	262:272	molar ratio	262:272	The monosaccharide composition and molar ratio of xanthan gum produced from glycerol are glucose: mannose: glucuronic acid=2.0:1.65:1.0.
27987957	2	19	theme	gum	285:287	arg1	glucose					316:322	glucose	316:322	glucose: mannose: glucuronic acid=2.0:1.65:1.0	316:361	The monosaccharide composition and molar ratio of xanthan gum produced from glycerol are glucose: mannose: glucuronic acid=2.0:1.65:1.0.
27987957	3	20	theme	gum	405:407	arg1	structure					384:392	Meanwhile, chemical structure	364:392	Meanwhile, chemical structure of xanthan gum produced from glycerol	364:430	Meanwhile, chemical structure of xanthan gum produced from glycerol is similar to that of the commercial xanthan through FT-IR and NMR.
27987957	4	21	theme	gum	544:546	arg1	weight					526:531	the molecular weight	512:531	the molecular weight of xanthan gum produced using our method (3.0±0.14×106Da)	512:589	Remarkably, the molecular weight of xanthan gum produced using our method (3.0±0.14×106Da) is about half that of the commercial one (5.8±0.25×106Da), and the consistency index (K) of which is less than 1/10 that of the commercial xanthan.
27987957	4	22	theme	one	628:630	arg1	half					600:603	half	600:603	half that of the commercial one (5.8±0.25×106Da)	600:647	Remarkably, the molecular weight of xanthan gum produced using our method (3.0±0.14×106Da) is about half that of the commercial one (5.8±0.25×106Da), and the consistency index (K) of which is less than 1/10 that of the commercial xanthan.
27987957	3	23	theme	Meanwhile	364:372	arg1	structure					384:392	Meanwhile, chemical structure	364:392	Meanwhile, chemical structure of xanthan gum produced from glycerol	364:430	Meanwhile, chemical structure of xanthan gum produced from glycerol is similar to that of the commercial xanthan through FT-IR and NMR.
27987957	4	24	theme	molecular	516:524	arg1	weight					526:531	the molecular weight	512:531	the molecular weight of xanthan gum produced using our method (3.0±0.14×106Da)	512:589	Remarkably, the molecular weight of xanthan gum produced using our method (3.0±0.14×106Da) is about half that of the commercial one (5.8±0.25×106Da), and the consistency index (K) of which is less than 1/10 that of the commercial xanthan.
27987957	1	25	theme	Xanthan	113:119	arg1	gum					121:123	Xanthan gum	113:123	Xanthan gum	113:123	Xanthan gum was produced by a mutant strain X. campestris CCTCC M2015714 with glycerol as the sole carbon source.
27987957	3	26	theme	xanthan	397:403	arg1	gum					405:407	xanthan gum	397:407	xanthan gum	397:407	Meanwhile, chemical structure of xanthan gum produced from glycerol is similar to that of the commercial xanthan through FT-IR and NMR.
27987957	4	27	dep	half	600:603	arg1	that					605:608	that	605:608	that	605:608	Remarkably, the molecular weight of xanthan gum produced using our method (3.0±0.14×106Da) is about half that of the commercial one (5.8±0.25×106Da), and the consistency index (K) of which is less than 1/10 that of the commercial xanthan.
27987957	4	28	theme	consistency	658:668	arg1	index					670:674	the consistency index	654:674	the consistency index (K) of which	654:687	Remarkably, the molecular weight of xanthan gum produced using our method (3.0±0.14×106Da) is about half that of the commercial one (5.8±0.25×106Da), and the consistency index (K) of which is less than 1/10 that of the commercial xanthan.
27987957	4	28	theme	consistency	658:668	arg1	xanthan					730:736	the commercial xanthan	715:736	the commercial xanthan	715:736	Remarkably, the molecular weight of xanthan gum produced using our method (3.0±0.14×106Da) is about half that of the commercial one (5.8±0.25×106Da), and the consistency index (K) of which is less than 1/10 that of the commercial xanthan.
27987957	4	28	theme	consistency	658:668	arg1	1/10					702:705	1/10	702:705	1/10	702:705	Remarkably, the molecular weight of xanthan gum produced using our method (3.0±0.14×106Da) is about half that of the commercial one (5.8±0.25×106Da), and the consistency index (K) of which is less than 1/10 that of the commercial xanthan.
27987957	4	28	theme	consistency	658:668	arg1	K					677:677	K	677:677	K	677:677	Remarkably, the molecular weight of xanthan gum produced using our method (3.0±0.14×106Da) is about half that of the commercial one (5.8±0.25×106Da), and the consistency index (K) of which is less than 1/10 that of the commercial xanthan.
27987957	4	29	theme	commercial	719:728	arg1	xanthan					730:736	the commercial xanthan	715:736	the commercial xanthan	715:736	Remarkably, the molecular weight of xanthan gum produced using our method (3.0±0.14×106Da) is about half that of the commercial one (5.8±0.25×106Da), and the consistency index (K) of which is less than 1/10 that of the commercial xanthan.
27987957	3	30	theme	commercial	458:467	arg1	xanthan					469:475	the commercial xanthan	454:475	the commercial xanthan through FT-IR and NMR	454:497	Meanwhile, chemical structure of xanthan gum produced from glycerol is similar to that of the commercial xanthan through FT-IR and NMR.
27987957	1	31	theme	sole	207:210	arg1	source					219:224	the sole carbon source	203:224	the sole carbon source	203:224	Xanthan gum was produced by a mutant strain X. campestris CCTCC M2015714 with glycerol as the sole carbon source.
27987957	5	32	from	glycerol	791:798	arg1	production					775:784	xanthan production	767:784	xanthan production from glycerol	767:798	This work paves the way for xanthan production from glycerol and is useful for studying the structure/application of xanthan gum.
27987957	5	33	theme	xanthan	856:862	arg1	gum					864:866	xanthan gum	856:866	xanthan gum	856:866	This work paves the way for xanthan production from glycerol and is useful for studying the structure/application of xanthan gum.
27987957	1	34	theme	carbon	212:217	arg1	source					219:224	the sole carbon source	203:224	the sole carbon source	203:224	Xanthan gum was produced by a mutant strain X. campestris CCTCC M2015714 with glycerol as the sole carbon source.
27987957	0	35	theme	mutant	60:65	arg1	strain					67:72	a mutant strain	58:72	a mutant strain Xanthomonas campestris CCTCC M2015714	58:110	Characterization of xanthan gum produced from glycerol by a mutant strain Xanthomonas campestris CCTCC M2015714.
27987957	5	36	theme	xanthan	767:773	arg1	production					775:784	xanthan production	767:784	xanthan production from glycerol	767:798	This work paves the way for xanthan production from glycerol and is useful for studying the structure/application of xanthan gum.
27987957	2	37	theme	monosaccharide	231:244	arg1	composition					246:256	monosaccharide composition	231:256	monosaccharide composition	231:256	The monosaccharide composition and molar ratio of xanthan gum produced from glycerol are glucose: mannose: glucuronic acid=2.0:1.65:1.0.
27987957	2	37	theme	monosaccharide	231:244	arg1	glucose					316:322	glucose	316:322	glucose: mannose: glucuronic acid=2.0:1.65:1.0	316:361	The monosaccharide composition and molar ratio of xanthan gum produced from glycerol are glucose: mannose: glucuronic acid=2.0:1.65:1.0.
27987957	2	37	theme	monosaccharide	231:244	arg1	ratio					268:272	molar ratio	262:272	molar ratio	262:272	The monosaccharide composition and molar ratio of xanthan gum produced from glycerol are glucose: mannose: glucuronic acid=2.0:1.65:1.0.
27987957	4	38	theme	commercial	617:626	arg1	one					628:630	the commercial one	613:630	the commercial one (5.8±0.25×106Da)	613:647	Remarkably, the molecular weight of xanthan gum produced using our method (3.0±0.14×106Da) is about half that of the commercial one (5.8±0.25×106Da), and the consistency index (K) of which is less than 1/10 that of the commercial xanthan.
27987957	4	38	theme	commercial	617:626	arg1	5.8±0.25×106Da					633:646	5.8±0.25×106Da	633:646	5.8±0.25×106Da	633:646	Remarkably, the molecular weight of xanthan gum produced using our method (3.0±0.14×106Da) is about half that of the commercial one (5.8±0.25×106Da), and the consistency index (K) of which is less than 1/10 that of the commercial xanthan.
27987957	0	39	theme	xanthan	20:26	arg1	gum					28:30	xanthan gum	20:30	xanthan gum	20:30	Characterization of xanthan gum produced from glycerol by a mutant strain Xanthomonas campestris CCTCC M2015714.
27987957	4	40	dep	1/10	702:705	arg1	that					707:710	that	707:710	that	707:710	Remarkably, the molecular weight of xanthan gum produced using our method (3.0±0.14×106Da) is about half that of the commercial one (5.8±0.25×106Da), and the consistency index (K) of which is less than 1/10 that of the commercial xanthan.
28790160	6	0	theme	→GF	942:944	arg1	microbiota					947:956	WT (WT→GF) or Card9-/- (Card9 →GF) microbiota	912:956	microbiota	947:956	To explore the impact of Card9-/-microbiota in infection susceptibility, GF WT mice were colonised with WT (WT→GF) or Card9-/- (Card9 →GF) microbiota before C. rodentium infection.
28790160	4	1	theme	infection	603:611	arg1	susceptibility					613:626	C. rodentium infection susceptibility	590:626	C. rodentium infection susceptibility	590:626	Here, we examined how CARD9 controls C. rodentium infection susceptibility through microbiota-dependent and microbiota-independent mechanisms.
28790160	12	2	theme	C.	1508:1509	arg1	rodentium					1511:1519	the monosaccharide-consuming C. rodentium	1479:1519	the monosaccharide-consuming C. rodentium	1479:1519	The microbiota of Card9-/- mice failed to outcompete the monosaccharide-consuming C. rodentium, worsening the infection severity.
28790160	6	3	theme	infection	855:863	arg1	susceptibility					865:878	infection susceptibility	855:878	infection susceptibility	855:878	To explore the impact of Card9-/-microbiota in infection susceptibility, GF WT mice were colonised with WT (WT→GF) or Card9-/- (Card9 →GF) microbiota before C. rodentium infection.
28790160	3	4	theme	enteric	416:422	arg1	rodentium					451:459	the enteric mouse pathogen Citrobacter rodentium	412:459	the enteric mouse pathogen Citrobacter rodentium that mimics human infections with enteropathogenic and enterohaemorrhagic Escherichia coli	412:550	Card9-/- mice are more susceptible to the enteric mouse pathogen Citrobacter rodentium that mimics human infections with enteropathogenic and enterohaemorrhagic Escherichia coli.
28790160	5	5	theme	DESIGN	696:701	arg1	infection					716:724	DESIGN C. rodentium infection	696:724	DESIGN C. rodentium infection	696:724	DESIGN C. rodentium infection was assessed in conventional and germ-free (GF) wild-type (WT) and Card9-/- mice.
28790160	6	6	theme	Card9-/-microbiota	833:850	arg1	impact					823:828	the impact	819:828	the impact of Card9-/-microbiota in infection susceptibility	819:878	To explore the impact of Card9-/-microbiota in infection susceptibility, GF WT mice were colonised with WT (WT→GF) or Card9-/- (Card9 →GF) microbiota before C. rodentium infection.
28790160	12	7	theme	infection	1536:1544	arg1	severity					1546:1553	the infection severity	1532:1553	the infection severity	1532:1553	The microbiota of Card9-/- mice failed to outcompete the monosaccharide-consuming C. rodentium, worsening the infection severity.
28790160	10	8	dep	CARD9	1228:1232	arg1	controls					1234:1241	controls	1234:1241	CARD9 controls pathogen virulence in a microbiota-independent manner by supporting a specific humoral response	1228:1337	RESULTS CARD9 controls pathogen virulence in a microbiota-independent manner by supporting a specific humoral response.
28790160	5	9	theme	rodentium	706:714	arg1	infection					716:724	DESIGN C. rodentium infection	696:724	DESIGN C. rodentium infection	696:724	DESIGN C. rodentium infection was assessed in conventional and germ-free (GF) wild-type (WT) and Card9-/- mice.
28790160	2	10	theme	innate	306:311	arg1	gene					322:325	a key innate immunity gene	300:325	a key innate immunity gene	300:325	Caspase recruitment domain 9 (CARD9), a key innate immunity gene, is required to shape a normal gut microbiota.
28790160	2	10	theme	innate	306:311	arg1	domain					282:287	Caspase recruitment domain 9	262:289	Caspase recruitment domain 9 (CARD9)	262:297	Caspase recruitment domain 9 (CARD9), a key innate immunity gene, is required to shape a normal gut microbiota.
28790160	15	11	theme	humoural	1983:1990	arg1	immunity					1992:1999	humoural immunity	1983:1999	humoural immunity	1983:1999	Genetic susceptibility to intestinal pathogens can be overridden by diet intervention that restores humoural immunity and a competing microbiota.
28790160	15	12	theme	diet	1951:1954	arg1	intervention					1956:1967	diet intervention	1951:1967	diet intervention that restores humoural immunity and a competing microbiota	1951:2026	Genetic susceptibility to intestinal pathogens can be overridden by diet intervention that restores humoural immunity and a competing microbiota.
28790160	2	13	theme	Caspase	262:268	arg1	gene					322:325	a key innate immunity gene	300:325	a key innate immunity gene	300:325	Caspase recruitment domain 9 (CARD9), a key innate immunity gene, is required to shape a normal gut microbiota.
28790160	2	13	theme	Caspase	262:268	arg1	domain					282:287	Caspase recruitment domain 9	262:289	Caspase recruitment domain 9 (CARD9)	262:297	Caspase recruitment domain 9 (CARD9), a key innate immunity gene, is required to shape a normal gut microbiota.
28790160	2	13	theme	Caspase	262:268	arg1	CARD9					292:296	CARD9	292:296	CARD9	292:296	Caspase recruitment domain 9 (CARD9), a key innate immunity gene, is required to shape a normal gut microbiota.
28790160	1	14	theme	intestinal	240:249	arg1	pathogens					251:259	intestinal pathogens	240:259	intestinal pathogens	240:259	OBJECTIVE In association with innate and adaptive immunity, the microbiota controls the colonisation resistance against intestinal pathogens.
28790160	6	15	theme	C.	965:966	arg1	infection					978:986	C. rodentium infection	965:986	C. rodentium infection	965:986	To explore the impact of Card9-/-microbiota in infection susceptibility, GF WT mice were colonised with WT (WT→GF) or Card9-/- (Card9 →GF) microbiota before C. rodentium infection.
28790160	12	16	theme	mice	1453:1456	arg1	microbiota					1430:1439	The microbiota	1426:1439	The microbiota of Card9-/- mice	1426:1456	The microbiota of Card9-/- mice failed to outcompete the monosaccharide-consuming C. rodentium, worsening the infection severity.
28790160	13	17	from	advantage	1615:1623	arg1	mice					1707:1710	Card9-/- mice	1698:1710	Card9-/- mice	1698:1710	A polysaccharide-enriched diet counteracted the ecological advantage of C. rodentium and the defective pathogen-specific antibody response in Card9-/- mice.
28790160	7	18	theme	rDNA	1034:1037	arg1	sequencing					1044:1053	rDNA gene sequencing	1034:1053	rDNA gene sequencing	1034:1053	Microbiota composition was determined by 16S rDNA gene sequencing.
28790160	10	19	theme	specific	1313:1320	arg1	response					1330:1337	a specific humoral response	1311:1337	a specific humoral response	1311:1337	RESULTS CARD9 controls pathogen virulence in a microbiota-independent manner by supporting a specific humoral response.
28790160	13	20	theme	defective	1649:1657	arg1	response					1686:1693	the defective pathogen-specific antibody response	1645:1693	the defective pathogen-specific antibody response in Card9-/- mice	1645:1710	A polysaccharide-enriched diet counteracted the ecological advantage of C. rodentium and the defective pathogen-specific antibody response in Card9-/- mice.
28790160	1	21	theme	innate	150:155	arg1	immunity					170:177	innate and adaptive immunity	150:177	innate and adaptive immunity	150:177	OBJECTIVE In association with innate and adaptive immunity, the microbiota controls the colonisation resistance against intestinal pathogens.
28790160	5	22	dep	wild-type	774:782	arg1	WT					785:786	WT	785:786	WT	785:786	DESIGN C. rodentium infection was assessed in conventional and germ-free (GF) wild-type (WT) and Card9-/- mice.
28790160	13	23	theme	antibody	1677:1684	arg1	response					1686:1693	the defective pathogen-specific antibody response	1645:1693	the defective pathogen-specific antibody response in Card9-/- mice	1645:1710	A polysaccharide-enriched diet counteracted the ecological advantage of C. rodentium and the defective pathogen-specific antibody response in Card9-/- mice.
28790160	1	24	theme	adaptive	161:168	arg1	immunity					170:177	innate and adaptive immunity	150:177	innate and adaptive immunity	150:177	OBJECTIVE In association with innate and adaptive immunity, the microbiota controls the colonisation resistance against intestinal pathogens.
28790160	11	25	theme	rodentium-induced	1368:1384	arg1	colitis					1386:1392	C. rodentium-induced colitis	1365:1392	C. rodentium-induced colitis	1365:1392	Higher susceptibility to C. rodentium-induced colitis was observed in Card9 →GF mice.
28790160	8	26	theme	Inflammation	1056:1067	arg1	severity					1069:1076	Inflammation severity	1056:1076	Inflammation severity	1056:1076	Inflammation severity was determined by histology score and lipocalin level.
28790160	7	27	theme	Microbiota	989:998	arg1	composition					1000:1010	Microbiota composition	989:1010	Microbiota composition	989:1010	Microbiota composition was determined by 16S rDNA gene sequencing.
28790160	5	28	dep	germ-free	759:767	arg1	GF					770:771	GF	770:771	GF	770:771	DESIGN C. rodentium infection was assessed in conventional and germ-free (GF) wild-type (WT) and Card9-/- mice.
28790160	0	29	theme	bacterial	99:107	arg1	virulence					109:117	bacterial virulence	99:117	bacterial virulence	99:117	Card9 mediates susceptibility to intestinal pathogens through microbiota modulation and control of bacterial virulence.
28790160	3	30	theme	pathogen	430:437	arg1	rodentium					451:459	the enteric mouse pathogen Citrobacter rodentium	412:459	the enteric mouse pathogen Citrobacter rodentium that mimics human infections with enteropathogenic and enterohaemorrhagic Escherichia coli	412:550	Card9-/- mice are more susceptible to the enteric mouse pathogen Citrobacter rodentium that mimics human infections with enteropathogenic and enterohaemorrhagic Escherichia coli.
28790160	5	31	theme	Card9-/-	793:800	arg1	mice					802:805	conventional and germ-free (GF) wild-type (WT) and Card9-/- mice	742:805	conventional and germ-free (GF) wild-type (WT) and Card9-/- mice	742:805	DESIGN C. rodentium infection was assessed in conventional and germ-free (GF) wild-type (WT) and Card9-/- mice.
28790160	9	32	theme	Microbiota-host	1133:1147	arg1	interactions					1163:1174	Microbiota-host immune system interactions	1133:1174	Microbiota-host immune system interactions	1133:1174	Microbiota-host immune system interactions were assessed by quantitative PCR analysis.
28790160	13	33	theme	rodentium	1631:1639	arg1	advantage					1615:1623	the ecological advantage	1600:1623	the ecological advantage of C. rodentium	1600:1639	A polysaccharide-enriched diet counteracted the ecological advantage of C. rodentium and the defective pathogen-specific antibody response in Card9-/- mice.
28790160	13	33	theme	rodentium	1631:1639	arg1	response					1686:1693	the defective pathogen-specific antibody response	1645:1693	the defective pathogen-specific antibody response in Card9-/- mice	1645:1710	A polysaccharide-enriched diet counteracted the ecological advantage of C. rodentium and the defective pathogen-specific antibody response in Card9-/- mice.
28790160	1	34	with	association	133:143	arg1	immunity					170:177	innate and adaptive immunity	150:177	innate and adaptive immunity	150:177	OBJECTIVE In association with innate and adaptive immunity, the microbiota controls the colonisation resistance against intestinal pathogens.
28790160	9	35	theme	system	1156:1161	arg1	interactions					1163:1174	Microbiota-host immune system interactions	1133:1174	Microbiota-host immune system interactions	1133:1174	Microbiota-host immune system interactions were assessed by quantitative PCR analysis.
28790160	8	36	theme	lipocalin	1116:1124	arg1	level					1126:1130	lipocalin level	1116:1130	lipocalin level	1116:1130	Inflammation severity was determined by histology score and lipocalin level.
28790160	13	37	theme	ecological	1604:1613	arg1	advantage					1615:1623	the ecological advantage	1600:1623	the ecological advantage of C. rodentium	1600:1639	A polysaccharide-enriched diet counteracted the ecological advantage of C. rodentium and the defective pathogen-specific antibody response in Card9-/- mice.
28790160	0	38	theme	intestinal	33:42	arg1	pathogens					44:52	intestinal pathogens	33:52	intestinal pathogens	33:52	Card9 mediates susceptibility to intestinal pathogens through microbiota modulation and control of bacterial virulence.
28790160	11	39	theme	Higher	1340:1345	arg1	susceptibility					1347:1360	Higher susceptibility	1340:1360	Higher susceptibility to C. rodentium-induced colitis	1340:1392	Higher susceptibility to C. rodentium-induced colitis was observed in Card9 →GF mice.
28790160	9	40	theme	PCR	1206:1208	arg1	analysis					1210:1217	quantitative PCR analysis	1193:1217	quantitative PCR analysis	1193:1217	Microbiota-host immune system interactions were assessed by quantitative PCR analysis.
28790160	13	41	theme	polysaccharide-enriched	1558:1580	arg1	diet					1582:1585	A polysaccharide-enriched diet	1556:1585	A polysaccharide-enriched diet	1556:1585	A polysaccharide-enriched diet counteracted the ecological advantage of C. rodentium and the defective pathogen-specific antibody response in Card9-/- mice.
28790160	1	42	from	OBJECTIVE	120:128	arg1	association					133:143	association	133:143	association with innate and adaptive immunity	133:177	OBJECTIVE In association with innate and adaptive immunity, the microbiota controls the colonisation resistance against intestinal pathogens.
28790160	6	43	theme	WT	884:885	arg1	mice					887:890	GF WT mice	881:890	GF WT mice	881:890	To explore the impact of Card9-/-microbiota in infection susceptibility, GF WT mice were colonised with WT (WT→GF) or Card9-/- (Card9 →GF) microbiota before C. rodentium infection.
28790160	4	44	theme	microbiota-dependent	636:655	arg1	mechanisms					684:693	microbiota-dependent and microbiota-independent mechanisms	636:693	microbiota-dependent and microbiota-independent mechanisms	636:693	Here, we examined how CARD9 controls C. rodentium infection susceptibility through microbiota-dependent and microbiota-independent mechanisms.
28790160	10	45	theme	pathogen	1243:1250	arg1	virulence					1252:1260	pathogen virulence	1243:1260	pathogen virulence	1243:1260	RESULTS CARD9 controls pathogen virulence in a microbiota-independent manner by supporting a specific humoral response.
28790160	4	46	theme	microbiota-independent	661:682	arg1	mechanisms					684:693	microbiota-dependent and microbiota-independent mechanisms	636:693	microbiota-dependent and microbiota-independent mechanisms	636:693	Here, we examined how CARD9 controls C. rodentium infection susceptibility through microbiota-dependent and microbiota-independent mechanisms.
28790160	2	47	theme	normal	351:356	arg1	microbiota					362:371	a normal gut microbiota	349:371	a normal gut microbiota	349:371	Caspase recruitment domain 9 (CARD9), a key innate immunity gene, is required to shape a normal gut microbiota.
28790160	6	48	theme	Card9	936:940	arg1	microbiota					947:956	WT (WT→GF) or Card9-/- (Card9 →GF) microbiota	912:956	microbiota	947:956	To explore the impact of Card9-/-microbiota in infection susceptibility, GF WT mice were colonised with WT (WT→GF) or Card9-/- (Card9 →GF) microbiota before C. rodentium infection.
28790160	4	49	theme	rodentium	593:601	arg1	infection					603:611	C. rodentium infection	590:611	C. rodentium infection susceptibility	590:626	Here, we examined how CARD9 controls C. rodentium infection susceptibility through microbiota-dependent and microbiota-independent mechanisms.
28790160	2	50	theme	key	302:304	arg1	gene					322:325	a key innate immunity gene	300:325	a key innate immunity gene	300:325	Caspase recruitment domain 9 (CARD9), a key innate immunity gene, is required to shape a normal gut microbiota.
28790160	2	50	theme	key	302:304	arg1	domain					282:287	Caspase recruitment domain 9	262:289	Caspase recruitment domain 9 (CARD9)	262:297	Caspase recruitment domain 9 (CARD9), a key innate immunity gene, is required to shape a normal gut microbiota.
28790160	10	51	theme	microbiota-independent	1267:1288	arg1	manner					1290:1295	a microbiota-independent manner	1265:1295	a microbiota-independent manner	1265:1295	RESULTS CARD9 controls pathogen virulence in a microbiota-independent manner by supporting a specific humoral response.
28790160	6	52	theme	Card9-/-	926:933	arg1	microbiota					947:956	WT (WT→GF) or Card9-/- (Card9 →GF) microbiota	912:956	microbiota	947:956	To explore the impact of Card9-/-microbiota in infection susceptibility, GF WT mice were colonised with WT (WT→GF) or Card9-/- (Card9 →GF) microbiota before C. rodentium infection.
28790160	12	53	theme	monosaccharide-consuming	1483:1506	arg1	rodentium					1511:1519	the monosaccharide-consuming C. rodentium	1479:1519	the monosaccharide-consuming C. rodentium	1479:1519	The microbiota of Card9-/- mice failed to outcompete the monosaccharide-consuming C. rodentium, worsening the infection severity.
28790160	3	54	with	infections	479:488	arg1	coli					547:550	Escherichia coli	535:550	Escherichia coli	535:550	Card9-/- mice are more susceptible to the enteric mouse pathogen Citrobacter rodentium that mimics human infections with enteropathogenic and enterohaemorrhagic Escherichia coli.
28790160	3	54	with	infections	479:488	arg1	enterohaemorrhagic					516:533	enterohaemorrhagic	516:533	enterohaemorrhagic	516:533	Card9-/- mice are more susceptible to the enteric mouse pathogen Citrobacter rodentium that mimics human infections with enteropathogenic and enterohaemorrhagic Escherichia coli.
28790160	15	55	theme	Genetic	1883:1889	arg1	susceptibility					1891:1904	Genetic susceptibility	1883:1904	Genetic susceptibility to intestinal pathogens	1883:1928	Genetic susceptibility to intestinal pathogens can be overridden by diet intervention that restores humoural immunity and a competing microbiota.
28790160	5	56	theme	C.	703:704	arg1	infection					716:724	DESIGN C. rodentium infection	696:724	DESIGN C. rodentium infection	696:724	DESIGN C. rodentium infection was assessed in conventional and germ-free (GF) wild-type (WT) and Card9-/- mice.
28790160	11	57	theme	→GF	1416:1418	arg1	mice					1420:1423	Card9 →GF mice	1410:1423	Card9 →GF mice	1410:1423	Higher susceptibility to C. rodentium-induced colitis was observed in Card9 →GF mice.
28790160	2	58	theme	immunity	313:320	arg1	gene					322:325	a key innate immunity gene	300:325	a key innate immunity gene	300:325	Caspase recruitment domain 9 (CARD9), a key innate immunity gene, is required to shape a normal gut microbiota.
28790160	2	58	theme	immunity	313:320	arg1	domain					282:287	Caspase recruitment domain 9	262:289	Caspase recruitment domain 9 (CARD9)	262:297	Caspase recruitment domain 9 (CARD9), a key innate immunity gene, is required to shape a normal gut microbiota.
28790160	1	59	theme	colonisation	208:219	arg1	resistance					221:230	the colonisation resistance	204:230	the colonisation resistance against intestinal pathogens	204:259	OBJECTIVE In association with innate and adaptive immunity, the microbiota controls the colonisation resistance against intestinal pathogens.
28790160	13	60	from	response	1686:1693	arg1	mice					1707:1710	Card9-/- mice	1698:1710	Card9-/- mice	1698:1710	A polysaccharide-enriched diet counteracted the ecological advantage of C. rodentium and the defective pathogen-specific antibody response in Card9-/- mice.
28790160	5	61	theme	wild-type	774:782	arg1	mice					802:805	conventional and germ-free (GF) wild-type (WT) and Card9-/- mice	742:805	conventional and germ-free (GF) wild-type (WT) and Card9-/- mice	742:805	DESIGN C. rodentium infection was assessed in conventional and germ-free (GF) wild-type (WT) and Card9-/- mice.
28790160	2	62	theme	recruitment	270:280	arg1	gene					322:325	a key innate immunity gene	300:325	a key innate immunity gene	300:325	Caspase recruitment domain 9 (CARD9), a key innate immunity gene, is required to shape a normal gut microbiota.
28790160	2	62	theme	recruitment	270:280	arg1	domain					282:287	Caspase recruitment domain 9	262:289	Caspase recruitment domain 9 (CARD9)	262:297	Caspase recruitment domain 9 (CARD9), a key innate immunity gene, is required to shape a normal gut microbiota.
28790160	2	62	theme	recruitment	270:280	arg1	CARD9					292:296	CARD9	292:296	CARD9	292:296	Caspase recruitment domain 9 (CARD9), a key innate immunity gene, is required to shape a normal gut microbiota.
28790160	6	63	theme	rodentium	968:976	arg1	infection					978:986	C. rodentium infection	965:986	C. rodentium infection	965:986	To explore the impact of Card9-/-microbiota in infection susceptibility, GF WT mice were colonised with WT (WT→GF) or Card9-/- (Card9 →GF) microbiota before C. rodentium infection.
28790160	14	64	theme	intestinal	1763:1772	arg1	infection					1774:1782	intestinal infection	1763:1782	intestinal infection	1763:1782	CONCLUSIONS CARD9 modulates the susceptibility to intestinal infection by controlling the pathogen virulence in a microbiota-dependent and microbiota-independent manner.
28790160	5	65	theme	conventional	742:753	arg1	mice					802:805	conventional and germ-free (GF) wild-type (WT) and Card9-/- mice	742:805	conventional and germ-free (GF) wild-type (WT) and Card9-/- mice	742:805	DESIGN C. rodentium infection was assessed in conventional and germ-free (GF) wild-type (WT) and Card9-/- mice.
28790160	13	66	theme	Card9-/-	1698:1705	arg1	mice					1707:1710	Card9-/- mice	1698:1710	Card9-/- mice	1698:1710	A polysaccharide-enriched diet counteracted the ecological advantage of C. rodentium and the defective pathogen-specific antibody response in Card9-/- mice.
28790160	12	67	theme	Card9-/-	1444:1451	arg1	mice					1453:1456	Card9-/- mice	1444:1456	Card9-/- mice	1444:1456	The microbiota of Card9-/- mice failed to outcompete the monosaccharide-consuming C. rodentium, worsening the infection severity.
28790160	10	68	theme	humoral	1322:1328	arg1	response					1330:1337	a specific humoral response	1311:1337	a specific humoral response	1311:1337	RESULTS CARD9 controls pathogen virulence in a microbiota-independent manner by supporting a specific humoral response.
28790160	5	69	theme	germ-free	759:767	arg1	mice					802:805	conventional and germ-free (GF) wild-type (WT) and Card9-/- mice	742:805	conventional and germ-free (GF) wild-type (WT) and Card9-/- mice	742:805	DESIGN C. rodentium infection was assessed in conventional and germ-free (GF) wild-type (WT) and Card9-/- mice.
28790160	14	70	theme	microbiota-independent	1852:1873	arg1	manner					1875:1880	a microbiota-dependent and microbiota-independent manner	1825:1880	a microbiota-dependent and microbiota-independent manner	1825:1880	CONCLUSIONS CARD9 modulates the susceptibility to intestinal infection by controlling the pathogen virulence in a microbiota-dependent and microbiota-independent manner.
28790160	7	71	theme	gene	1039:1042	arg1	sequencing					1044:1053	rDNA gene sequencing	1034:1053	rDNA gene sequencing	1034:1053	Microbiota composition was determined by 16S rDNA gene sequencing.
28790160	11	72	located	observed	1398:1405	arg2	susceptibility					1347:1360	Higher susceptibility	1340:1360	Higher susceptibility to C. rodentium-induced colitis	1340:1392	Higher susceptibility to C. rodentium-induced colitis was observed in Card9 →GF mice.
28790160	11	72	located	observed	1398:1405	arg1	mice					1420:1423	Card9 →GF mice	1410:1423	Card9 →GF mice	1410:1423	Higher susceptibility to C. rodentium-induced colitis was observed in Card9 →GF mice.
28790160	14	73	theme	microbiota-dependent	1827:1846	arg1	manner					1875:1880	a microbiota-dependent and microbiota-independent manner	1825:1880	a microbiota-dependent and microbiota-independent manner	1825:1880	CONCLUSIONS CARD9 modulates the susceptibility to intestinal infection by controlling the pathogen virulence in a microbiota-dependent and microbiota-independent manner.
28790160	13	74	theme	pathogen-specific	1659:1675	arg1	response					1686:1693	the defective pathogen-specific antibody response	1645:1693	the defective pathogen-specific antibody response in Card9-/- mice	1645:1710	A polysaccharide-enriched diet counteracted the ecological advantage of C. rodentium and the defective pathogen-specific antibody response in Card9-/- mice.
28790160	14	75	theme	CONCLUSIONS	1713:1723	arg1	CARD9					1725:1729	CONCLUSIONS CARD9	1713:1729	CONCLUSIONS CARD9	1713:1729	CONCLUSIONS CARD9 modulates the susceptibility to intestinal infection by controlling the pathogen virulence in a microbiota-dependent and microbiota-independent manner.
28790160	15	76	theme	intestinal	1909:1918	arg1	pathogens					1920:1928	intestinal pathogens	1909:1928	intestinal pathogens	1909:1928	Genetic susceptibility to intestinal pathogens can be overridden by diet intervention that restores humoural immunity and a competing microbiota.
28790160	0	77	theme	virulence	109:117	arg1	control					88:94	control	88:94	control	88:94	Card9 mediates susceptibility to intestinal pathogens through microbiota modulation and control of bacterial virulence.
28790160	0	77	theme	virulence	109:117	arg1	modulation					73:82	microbiota modulation	62:82	microbiota modulation	62:82	Card9 mediates susceptibility to intestinal pathogens through microbiota modulation and control of bacterial virulence.
28790160	14	78	theme	pathogen	1803:1810	arg1	virulence					1812:1820	the pathogen virulence	1799:1820	the pathogen virulence	1799:1820	CONCLUSIONS CARD9 modulates the susceptibility to intestinal infection by controlling the pathogen virulence in a microbiota-dependent and microbiota-independent manner.
28790160	11	79	theme	C.	1365:1366	arg1	colitis					1386:1392	C. rodentium-induced colitis	1365:1392	C. rodentium-induced colitis	1365:1392	Higher susceptibility to C. rodentium-induced colitis was observed in Card9 →GF mice.
28790160	15	80	theme	competing	2007:2015	arg1	microbiota					2017:2026	a competing microbiota	2005:2026	a competing microbiota	2005:2026	Genetic susceptibility to intestinal pathogens can be overridden by diet intervention that restores humoural immunity and a competing microbiota.
28790160	8	81	theme	histology	1096:1104	arg1	score					1106:1110	histology score	1096:1110	histology score	1096:1110	Inflammation severity was determined by histology score and lipocalin level.
28790160	11	82	theme	Card9	1410:1414	arg1	mice					1420:1423	Card9 →GF mice	1410:1423	Card9 →GF mice	1410:1423	Higher susceptibility to C. rodentium-induced colitis was observed in Card9 →GF mice.
28790160	3	83	theme	Citrobacter	439:449	arg1	rodentium					451:459	the enteric mouse pathogen Citrobacter rodentium	412:459	the enteric mouse pathogen Citrobacter rodentium that mimics human infections with enteropathogenic and enterohaemorrhagic Escherichia coli	412:550	Card9-/- mice are more susceptible to the enteric mouse pathogen Citrobacter rodentium that mimics human infections with enteropathogenic and enterohaemorrhagic Escherichia coli.
28790160	9	84	theme	immune	1149:1154	arg1	interactions					1163:1174	Microbiota-host immune system interactions	1133:1174	Microbiota-host immune system interactions	1133:1174	Microbiota-host immune system interactions were assessed by quantitative PCR analysis.
28790160	3	85	theme	mouse	424:428	arg1	rodentium					451:459	the enteric mouse pathogen Citrobacter rodentium	412:459	the enteric mouse pathogen Citrobacter rodentium that mimics human infections with enteropathogenic and enterohaemorrhagic Escherichia coli	412:550	Card9-/- mice are more susceptible to the enteric mouse pathogen Citrobacter rodentium that mimics human infections with enteropathogenic and enterohaemorrhagic Escherichia coli.
28790160	1	86	from	association	133:143	arg1	OBJECTIVE					120:128	OBJECTIVE	120:128	OBJECTIVE	120:128	OBJECTIVE In association with innate and adaptive immunity, the microbiota controls the colonisation resistance against intestinal pathogens.
28790160	6	87	from	impact	823:828	arg1	susceptibility					865:878	infection susceptibility	855:878	infection susceptibility	855:878	To explore the impact of Card9-/-microbiota in infection susceptibility, GF WT mice were colonised with WT (WT→GF) or Card9-/- (Card9 →GF) microbiota before C. rodentium infection.
28790160	3	88	theme	Card9-/-	374:381	arg1	mice					383:386	Card9-/- mice	374:386	Card9-/- mice	374:386	Card9-/- mice are more susceptible to the enteric mouse pathogen Citrobacter rodentium that mimics human infections with enteropathogenic and enterohaemorrhagic Escherichia coli.
28790160	4	89	theme	C.	590:591	arg1	infection					603:611	C. rodentium infection	590:611	C. rodentium infection susceptibility	590:626	Here, we examined how CARD9 controls C. rodentium infection susceptibility through microbiota-dependent and microbiota-independent mechanisms.
28790160	3	90	theme	human	473:477	arg1	infections					479:488	human infections	473:488	human infections with enteropathogenic and enterohaemorrhagic Escherichia coli	473:550	Card9-/- mice are more susceptible to the enteric mouse pathogen Citrobacter rodentium that mimics human infections with enteropathogenic and enterohaemorrhagic Escherichia coli.
28790160	6	91	theme	WT	912:913	arg1	WT→GF					916:920	WT (WT→GF) or Card9-/- (Card9 →GF) microbiota	912:956	WT→GF	916:920	To explore the impact of Card9-/-microbiota in infection susceptibility, GF WT mice were colonised with WT (WT→GF) or Card9-/- (Card9 →GF) microbiota before C. rodentium infection.
28790160	0	92	theme	microbiota	62:71	arg1	modulation					73:82	microbiota modulation	62:82	microbiota modulation	62:82	Card9 mediates susceptibility to intestinal pathogens through microbiota modulation and control of bacterial virulence.
28790160	9	93	theme	quantitative	1193:1204	arg1	analysis					1210:1217	quantitative PCR analysis	1193:1217	quantitative PCR analysis	1193:1217	Microbiota-host immune system interactions were assessed by quantitative PCR analysis.
28790160	6	94	theme	GF	881:882	arg1	mice					887:890	GF WT mice	881:890	GF WT mice	881:890	To explore the impact of Card9-/-microbiota in infection susceptibility, GF WT mice were colonised with WT (WT→GF) or Card9-/- (Card9 →GF) microbiota before C. rodentium infection.
28790160	13	95	theme	C.	1628:1629	arg1	rodentium					1631:1639	C. rodentium	1628:1639	C. rodentium	1628:1639	A polysaccharide-enriched diet counteracted the ecological advantage of C. rodentium and the defective pathogen-specific antibody response in Card9-/- mice.
28790160	2	96	theme	gut	358:360	arg1	microbiota					362:371	a normal gut microbiota	349:371	a normal gut microbiota	349:371	Caspase recruitment domain 9 (CARD9), a key innate immunity gene, is required to shape a normal gut microbiota.
28419963	6	0	theme	organ	760:764	arg1	weights					744:750	The relative weights	731:750	The relative weights of main organ, and biochemical indicators	731:792	The relative weights of main organ, and biochemical indicators also did not markedly change.
28419963	7	1	theme	increase	936:943	arg1	activities					949:958	increase LDH activities	936:958	increase LDH activities	936:958	PCS can significantly prolong the duration of the swimming time to exhaustion in mice, decrease BUN, LA levels, increase LDH activities, and the contents of HG in the PCS treated mice.
28419963	7	2	theme	swimming	874:881	arg1	time					883:886	the swimming time	870:886	the swimming time to exhaustion	870:900	PCS can significantly prolong the duration of the swimming time to exhaustion in mice, decrease BUN, LA levels, increase LDH activities, and the contents of HG in the PCS treated mice.
28419963	0	3	from	toxicity	6:13	arg1	silk					82:85	corn silk	77:85	corn silk	77:85	Acute toxicity and anti-fatigue activity of polysaccharide-rich extract from corn silk.
28419963	5	4	theme	toxicity	541:548	arg1	mortality					507:515	No mortality	504:515	No mortality	504:515	No mortality and general symptoms of toxicity were observed in the PCS treated mice (7.5, 15, and 20g/kg body weight), the body weight and food consumption were not significantly changed compared with the normal control group.
28419963	5	4	theme	toxicity	541:548	arg1	symptoms					529:536	general symptoms	521:536	general symptoms	521:536	No mortality and general symptoms of toxicity were observed in the PCS treated mice (7.5, 15, and 20g/kg body weight), the body weight and food consumption were not significantly changed compared with the normal control group.
28419963	8	5	theme	female	1105:1110	arg1	mice					1112:1115	male and female mice	1096:1115	male and female mice	1096:1115	The dose of 400mg/kg body weight is the optimal dose for anti-fatigue activity both in male and female mice.
28419963	8	6	theme	400mg/kg	1021:1028	arg1	weight					1035:1040	400mg/kg body weight	1021:1040	400mg/kg body weight	1021:1040	The dose of 400mg/kg body weight is the optimal dose for anti-fatigue activity both in male and female mice.
28419963	7	7	theme	treated	995:1001	arg1	mice					1003:1006	the PCS treated mice	987:1006	the PCS treated mice	987:1006	PCS can significantly prolong the duration of the swimming time to exhaustion in mice, decrease BUN, LA levels, increase LDH activities, and the contents of HG in the PCS treated mice.
28419963	2	8	theme	chemical	270:277	arg1	compositions					279:290	its chemical compositions	266:290	its chemical compositions of monosaccharide	266:308	PCS was prepared by water extracting-alcohol precipitating method, and its chemical compositions of monosaccharide were analyzed.
28419963	3	9	theme	anti-fatigue	350:361	arg1	activity					363:370	anti-fatigue activity	350:370	anti-fatigue activity	350:370	Then, acute toxicity and anti-fatigue activity of PCS were evaluated.
28419963	7	10	from	contents	969:976	arg1	mice					1003:1006	the PCS treated mice	987:1006	the PCS treated mice	987:1006	PCS can significantly prolong the duration of the swimming time to exhaustion in mice, decrease BUN, LA levels, increase LDH activities, and the contents of HG in the PCS treated mice.
28419963	7	11	dep	prolong	846:852	arg1	decrease					911:918	decrease	911:918	decrease BUN, LA levels, increase LDH activities, and the contents of HG in the PCS treated mice	911:1006	PCS can significantly prolong the duration of the swimming time to exhaustion in mice, decrease BUN, LA levels, increase LDH activities, and the contents of HG in the PCS treated mice.
28419963	3	12	theme	acute	331:335	arg1	toxicity					337:344	acute toxicity	331:344	acute toxicity	331:344	Then, acute toxicity and anti-fatigue activity of PCS were evaluated.
28419963	6	13	theme	relative	735:742	arg1	weights					744:750	The relative weights	731:750	The relative weights of main organ, and biochemical indicators	731:792	The relative weights of main organ, and biochemical indicators also did not markedly change.
28419963	5	14	theme	No	504:505	arg1	mortality					507:515	No mortality	504:515	No mortality	504:515	No mortality and general symptoms of toxicity were observed in the PCS treated mice (7.5, 15, and 20g/kg body weight), the body weight and food consumption were not significantly changed compared with the normal control group.
28419963	8	15	theme	body	1030:1033	arg1	weight					1035:1040	400mg/kg body weight	1021:1040	400mg/kg body weight	1021:1040	The dose of 400mg/kg body weight is the optimal dose for anti-fatigue activity both in male and female mice.
28419963	7	16	theme	PCS	991:993	arg1	mice					1003:1006	the PCS treated mice	987:1006	the PCS treated mice	987:1006	PCS can significantly prolong the duration of the swimming time to exhaustion in mice, decrease BUN, LA levels, increase LDH activities, and the contents of HG in the PCS treated mice.
28419963	7	17	from	HG	981:982	arg1	mice					1003:1006	the PCS treated mice	987:1006	the PCS treated mice	987:1006	PCS can significantly prolong the duration of the swimming time to exhaustion in mice, decrease BUN, LA levels, increase LDH activities, and the contents of HG in the PCS treated mice.
28419963	7	18	theme	LA	925:926	arg1	levels					928:933	LA levels	925:933	LA levels	925:933	PCS can significantly prolong the duration of the swimming time to exhaustion in mice, decrease BUN, LA levels, increase LDH activities, and the contents of HG in the PCS treated mice.
28419963	5	19	dep	observed	555:562	arg1	weight					614:619	7.5, 15, and 20g/kg body weight	589:619	7.5, 15, and 20g/kg body weight	589:619	No mortality and general symptoms of toxicity were observed in the PCS treated mice (7.5, 15, and 20g/kg body weight), the body weight and food consumption were not significantly changed compared with the normal control group.
28419963	1	20	theme	PCS	154:156	arg1	safety					130:135	safety	130:135	safety	130:135	The aim of this study was to evaluate the safety and potential of PCS as the anti-fatigue functional food.
28419963	1	20	theme	PCS	154:156	arg1	food					189:192	the anti-fatigue functional food	161:192	the anti-fatigue functional food	161:192	The aim of this study was to evaluate the safety and potential of PCS as the anti-fatigue functional food.
28419963	1	20	theme	PCS	154:156	arg1	potential					141:149	potential	141:149	potential	141:149	The aim of this study was to evaluate the safety and potential of PCS as the anti-fatigue functional food.
28419963	3	21	theme	PCS	375:377	arg1	toxicity					337:344	acute toxicity	331:344	acute toxicity	331:344	Then, acute toxicity and anti-fatigue activity of PCS were evaluated.
28419963	3	21	theme	PCS	375:377	arg1	activity					363:370	anti-fatigue activity	350:370	anti-fatigue activity	350:370	Then, acute toxicity and anti-fatigue activity of PCS were evaluated.
28419963	0	22	from	activity	32:39	arg1	silk					82:85	corn silk	77:85	corn silk	77:85	Acute toxicity and anti-fatigue activity of polysaccharide-rich extract from corn silk.
28419963	5	23	theme	general	521:527	arg1	symptoms					529:536	general symptoms	521:536	general symptoms	521:536	No mortality and general symptoms of toxicity were observed in the PCS treated mice (7.5, 15, and 20g/kg body weight), the body weight and food consumption were not significantly changed compared with the normal control group.
28419963	5	24	theme	body	609:612	arg1	weight					614:619	7.5, 15, and 20g/kg body weight	589:619	7.5, 15, and 20g/kg body weight	589:619	No mortality and general symptoms of toxicity were observed in the PCS treated mice (7.5, 15, and 20g/kg body weight), the body weight and food consumption were not significantly changed compared with the normal control group.
28419963	7	25	theme	HG	981:982	arg1	levels					928:933	LA levels	925:933	LA levels	925:933	PCS can significantly prolong the duration of the swimming time to exhaustion in mice, decrease BUN, LA levels, increase LDH activities, and the contents of HG in the PCS treated mice.
28419963	7	25	theme	HG	981:982	arg1	activities					949:958	increase LDH activities	936:958	increase LDH activities	936:958	PCS can significantly prolong the duration of the swimming time to exhaustion in mice, decrease BUN, LA levels, increase LDH activities, and the contents of HG in the PCS treated mice.
28419963	7	25	theme	HG	981:982	arg1	BUN					920:922	BUN	920:922	BUN	920:922	PCS can significantly prolong the duration of the swimming time to exhaustion in mice, decrease BUN, LA levels, increase LDH activities, and the contents of HG in the PCS treated mice.
28419963	7	25	theme	HG	981:982	arg1	contents					969:976	the contents	965:976	the contents of HG in the PCS treated mice	965:1006	PCS can significantly prolong the duration of the swimming time to exhaustion in mice, decrease BUN, LA levels, increase LDH activities, and the contents of HG in the PCS treated mice.
28419963	5	26	theme	control	716:722	arg1	group					724:728	the normal control group	705:728	the normal control group	705:728	No mortality and general symptoms of toxicity were observed in the PCS treated mice (7.5, 15, and 20g/kg body weight), the body weight and food consumption were not significantly changed compared with the normal control group.
28419963	0	27	theme	Acute	0:4	arg1	toxicity					6:13	Acute toxicity	0:13	Acute toxicity	0:13	Acute toxicity and anti-fatigue activity of polysaccharide-rich extract from corn silk.
28419963	2	28	theme	monosaccharide	295:308	arg1	compositions					279:290	its chemical compositions	266:290	its chemical compositions of monosaccharide	266:308	PCS was prepared by water extracting-alcohol precipitating method, and its chemical compositions of monosaccharide were analyzed.
28419963	7	29	theme	LDH	945:947	arg1	activities					949:958	increase LDH activities	936:958	increase LDH activities	936:958	PCS can significantly prolong the duration of the swimming time to exhaustion in mice, decrease BUN, LA levels, increase LDH activities, and the contents of HG in the PCS treated mice.
28419963	0	30	theme	anti-fatigue	19:30	arg1	activity					32:39	anti-fatigue activity	19:39	anti-fatigue activity	19:39	Acute toxicity and anti-fatigue activity of polysaccharide-rich extract from corn silk.
28419963	7	31	from	BUN	920:922	arg1	mice					1003:1006	the PCS treated mice	987:1006	the PCS treated mice	987:1006	PCS can significantly prolong the duration of the swimming time to exhaustion in mice, decrease BUN, LA levels, increase LDH activities, and the contents of HG in the PCS treated mice.
28419963	9	32	theme	high	1224:1227	arg1	safety					1229:1234	high safety	1224:1234	high safety	1224:1234	In conclusion, PCS is a promising traditional natural-based therapeutic remedy for relieving fatigue with high safety.
28419963	9	33	from	remedy	1190:1195	arg1	conclusion					1121:1130	conclusion	1121:1130	conclusion	1121:1130	In conclusion, PCS is a promising traditional natural-based therapeutic remedy for relieving fatigue with high safety.
28419963	1	34	theme	anti-fatigue	165:176	arg1	safety					130:135	safety	130:135	safety	130:135	The aim of this study was to evaluate the safety and potential of PCS as the anti-fatigue functional food.
28419963	1	34	theme	anti-fatigue	165:176	arg1	food					189:192	the anti-fatigue functional food	161:192	the anti-fatigue functional food	161:192	The aim of this study was to evaluate the safety and potential of PCS as the anti-fatigue functional food.
28419963	1	34	theme	anti-fatigue	165:176	arg1	potential					141:149	potential	141:149	potential	141:149	The aim of this study was to evaluate the safety and potential of PCS as the anti-fatigue functional food.
28419963	4	35	dep	1.00	492:495	arg1	0.57					498:501	0.57	498:501	0.57	498:501	PCS is composed of Rha, Arab, Xyl, Man, Glu, and Gal, its molar ratio is 0.17: 0.30: 0.26: 0.35: 1.00: 0.57.
28419963	1	36	dep	safety	130:135	arg1	the					126:128	the	126:128	the	126:128	The aim of this study was to evaluate the safety and potential of PCS as the anti-fatigue functional food.
28419963	1	37	theme	functional	178:187	arg1	safety					130:135	safety	130:135	safety	130:135	The aim of this study was to evaluate the safety and potential of PCS as the anti-fatigue functional food.
28419963	1	37	theme	functional	178:187	arg1	food					189:192	the anti-fatigue functional food	161:192	the anti-fatigue functional food	161:192	The aim of this study was to evaluate the safety and potential of PCS as the anti-fatigue functional food.
28419963	1	37	theme	functional	178:187	arg1	potential					141:149	potential	141:149	potential	141:149	The aim of this study was to evaluate the safety and potential of PCS as the anti-fatigue functional food.
28419963	0	38	from	silk	82:85	arg1	toxicity					6:13	Acute toxicity	0:13	Acute toxicity	0:13	Acute toxicity and anti-fatigue activity of polysaccharide-rich extract from corn silk.
28419963	0	38	from	silk	82:85	arg1	activity					32:39	anti-fatigue activity	19:39	anti-fatigue activity	19:39	Acute toxicity and anti-fatigue activity of polysaccharide-rich extract from corn silk.
28419963	0	38	from	silk	82:85	arg1	extract					64:70	polysaccharide-rich extract	44:70	polysaccharide-rich extract from corn silk	44:85	Acute toxicity and anti-fatigue activity of polysaccharide-rich extract from corn silk.
28419963	8	39	theme	weight	1035:1040	arg1	dose					1057:1060	the optimal dose	1045:1060	the optimal dose for anti-fatigue activity both in male and female mice	1045:1115	The dose of 400mg/kg body weight is the optimal dose for anti-fatigue activity both in male and female mice.
28419963	8	39	theme	weight	1035:1040	arg1	dose					1013:1016	The dose	1009:1016	The dose of 400mg/kg body weight	1009:1040	The dose of 400mg/kg body weight is the optimal dose for anti-fatigue activity both in male and female mice.
28419963	5	40	theme	body	627:630	arg1	weight					632:637	the body weight	623:637	the body weight	623:637	No mortality and general symptoms of toxicity were observed in the PCS treated mice (7.5, 15, and 20g/kg body weight), the body weight and food consumption were not significantly changed compared with the normal control group.
28419963	7	41	from	levels	928:933	arg1	mice					1003:1006	the PCS treated mice	987:1006	the PCS treated mice	987:1006	PCS can significantly prolong the duration of the swimming time to exhaustion in mice, decrease BUN, LA levels, increase LDH activities, and the contents of HG in the PCS treated mice.
28419963	0	42	theme	extract	64:70	arg1	toxicity					6:13	Acute toxicity	0:13	Acute toxicity	0:13	Acute toxicity and anti-fatigue activity of polysaccharide-rich extract from corn silk.
28419963	0	42	theme	extract	64:70	arg1	activity					32:39	anti-fatigue activity	19:39	anti-fatigue activity	19:39	Acute toxicity and anti-fatigue activity of polysaccharide-rich extract from corn silk.
28419963	4	43	theme	molar	453:457	arg1	ratio					459:463	its molar ratio	449:463	its molar ratio	449:463	PCS is composed of Rha, Arab, Xyl, Man, Glu, and Gal, its molar ratio is 0.17: 0.30: 0.26: 0.35: 1.00: 0.57.
28419963	7	44	theme	time	883:886	arg1	duration					858:865	the duration	854:865	the duration of the swimming time to exhaustion	854:900	PCS can significantly prolong the duration of the swimming time to exhaustion in mice, decrease BUN, LA levels, increase LDH activities, and the contents of HG in the PCS treated mice.
28419963	7	45	from	mice	1003:1006	arg1	levels					928:933	LA levels	925:933	LA levels	925:933	PCS can significantly prolong the duration of the swimming time to exhaustion in mice, decrease BUN, LA levels, increase LDH activities, and the contents of HG in the PCS treated mice.
28419963	7	45	from	mice	1003:1006	arg1	activities					949:958	increase LDH activities	936:958	increase LDH activities	936:958	PCS can significantly prolong the duration of the swimming time to exhaustion in mice, decrease BUN, LA levels, increase LDH activities, and the contents of HG in the PCS treated mice.
28419963	7	45	from	mice	1003:1006	arg1	BUN					920:922	BUN	920:922	BUN	920:922	PCS can significantly prolong the duration of the swimming time to exhaustion in mice, decrease BUN, LA levels, increase LDH activities, and the contents of HG in the PCS treated mice.
28419963	7	45	from	mice	1003:1006	arg1	contents					969:976	the contents	965:976	the contents of HG in the PCS treated mice	965:1006	PCS can significantly prolong the duration of the swimming time to exhaustion in mice, decrease BUN, LA levels, increase LDH activities, and the contents of HG in the PCS treated mice.
28419963	1	46	theme	study	104:108	arg1	aim					92:94	The aim	88:94	The aim of this study	88:108	The aim of this study was to evaluate the safety and potential of PCS as the anti-fatigue functional food.
28419963	0	47	theme	polysaccharide-rich	44:62	arg1	extract					64:70	polysaccharide-rich extract	44:70	polysaccharide-rich extract from corn silk	44:85	Acute toxicity and anti-fatigue activity of polysaccharide-rich extract from corn silk.
28419963	6	48	theme	biochemical	771:781	arg1	indicators					783:792	biochemical indicators	771:792	biochemical indicators	771:792	The relative weights of main organ, and biochemical indicators also did not markedly change.
28419963	8	49	dep	activity	1079:1086	arg1	both					1088:1091	both	1088:1091	both	1088:1091	The dose of 400mg/kg body weight is the optimal dose for anti-fatigue activity both in male and female mice.
28419963	6	50	theme	main	755:758	arg1	organ					760:764	main organ	755:764	main organ	755:764	The relative weights of main organ, and biochemical indicators also did not markedly change.
28419963	8	51	theme	anti-fatigue	1066:1077	arg1	activity					1079:1086	anti-fatigue activity	1066:1086	anti-fatigue activity	1066:1086	The dose of 400mg/kg body weight is the optimal dose for anti-fatigue activity both in male and female mice.
28419963	9	52	theme	promising	1142:1150	arg1	remedy					1190:1195	a promising traditional natural-based therapeutic remedy	1140:1195	a promising traditional natural-based therapeutic remedy for relieving fatigue with high safety	1140:1234	In conclusion, PCS is a promising traditional natural-based therapeutic remedy for relieving fatigue with high safety.
28419963	9	52	theme	promising	1142:1150	arg1	PCS					1133:1135	PCS	1133:1135	PCS	1133:1135	In conclusion, PCS is a promising traditional natural-based therapeutic remedy for relieving fatigue with high safety.
28419963	5	53	theme	PCS	571:573	arg1	mice					583:586	the PCS treated mice	567:586	the PCS treated mice	567:586	No mortality and general symptoms of toxicity were observed in the PCS treated mice (7.5, 15, and 20g/kg body weight), the body weight and food consumption were not significantly changed compared with the normal control group.
28419963	9	54	theme	traditional	1152:1162	arg1	remedy					1190:1195	a promising traditional natural-based therapeutic remedy	1140:1195	a promising traditional natural-based therapeutic remedy for relieving fatigue with high safety	1140:1234	In conclusion, PCS is a promising traditional natural-based therapeutic remedy for relieving fatigue with high safety.
28419963	9	54	theme	traditional	1152:1162	arg1	PCS					1133:1135	PCS	1133:1135	PCS	1133:1135	In conclusion, PCS is a promising traditional natural-based therapeutic remedy for relieving fatigue with high safety.
28419963	5	55	theme	treated	575:581	arg1	mice					583:586	the PCS treated mice	567:586	the PCS treated mice	567:586	No mortality and general symptoms of toxicity were observed in the PCS treated mice (7.5, 15, and 20g/kg body weight), the body weight and food consumption were not significantly changed compared with the normal control group.
28419963	2	56	theme	precipitating	240:252	arg1	method					254:259	water extracting-alcohol precipitating method	215:259	water extracting-alcohol precipitating method	215:259	PCS was prepared by water extracting-alcohol precipitating method, and its chemical compositions of monosaccharide were analyzed.
28419963	8	57	theme	male	1096:1099	arg1	mice					1112:1115	male and female mice	1096:1115	male and female mice	1096:1115	The dose of 400mg/kg body weight is the optimal dose for anti-fatigue activity both in male and female mice.
28419963	5	58	located	observed	555:562	arg2	mortality					507:515	No mortality	504:515	No mortality	504:515	No mortality and general symptoms of toxicity were observed in the PCS treated mice (7.5, 15, and 20g/kg body weight), the body weight and food consumption were not significantly changed compared with the normal control group.
28419963	5	58	located	observed	555:562	arg2	symptoms					529:536	general symptoms	521:536	general symptoms	521:536	No mortality and general symptoms of toxicity were observed in the PCS treated mice (7.5, 15, and 20g/kg body weight), the body weight and food consumption were not significantly changed compared with the normal control group.
28419963	5	58	located	observed	555:562	arg1	mice					583:586	the PCS treated mice	567:586	the PCS treated mice	567:586	No mortality and general symptoms of toxicity were observed in the PCS treated mice (7.5, 15, and 20g/kg body weight), the body weight and food consumption were not significantly changed compared with the normal control group.
28419963	9	59	theme	natural-based	1164:1176	arg1	remedy					1190:1195	a promising traditional natural-based therapeutic remedy	1140:1195	a promising traditional natural-based therapeutic remedy for relieving fatigue with high safety	1140:1234	In conclusion, PCS is a promising traditional natural-based therapeutic remedy for relieving fatigue with high safety.
28419963	9	59	theme	natural-based	1164:1176	arg1	PCS					1133:1135	PCS	1133:1135	PCS	1133:1135	In conclusion, PCS is a promising traditional natural-based therapeutic remedy for relieving fatigue with high safety.
28419963	0	60	theme	corn	77:80	arg1	silk					82:85	corn silk	77:85	corn silk	77:85	Acute toxicity and anti-fatigue activity of polysaccharide-rich extract from corn silk.
28419963	2	61	theme	extracting-alcohol	221:238	arg1	method					254:259	water extracting-alcohol precipitating method	215:259	water extracting-alcohol precipitating method	215:259	PCS was prepared by water extracting-alcohol precipitating method, and its chemical compositions of monosaccharide were analyzed.
28419963	8	62	theme	optimal	1049:1055	arg1	dose					1013:1016	The dose	1009:1016	The dose of 400mg/kg body weight	1009:1040	The dose of 400mg/kg body weight is the optimal dose for anti-fatigue activity both in male and female mice.
28419963	8	62	theme	optimal	1049:1055	arg1	dose					1057:1060	the optimal dose	1045:1060	the optimal dose for anti-fatigue activity both in male and female mice	1045:1115	The dose of 400mg/kg body weight is the optimal dose for anti-fatigue activity both in male and female mice.
28419963	5	63	theme	normal	709:714	arg1	group					724:728	the normal control group	705:728	the normal control group	705:728	No mortality and general symptoms of toxicity were observed in the PCS treated mice (7.5, 15, and 20g/kg body weight), the body weight and food consumption were not significantly changed compared with the normal control group.
28419963	9	64	theme	therapeutic	1178:1188	arg1	remedy					1190:1195	a promising traditional natural-based therapeutic remedy	1140:1195	a promising traditional natural-based therapeutic remedy for relieving fatigue with high safety	1140:1234	In conclusion, PCS is a promising traditional natural-based therapeutic remedy for relieving fatigue with high safety.
28419963	9	64	theme	therapeutic	1178:1188	arg1	PCS					1133:1135	PCS	1133:1135	PCS	1133:1135	In conclusion, PCS is a promising traditional natural-based therapeutic remedy for relieving fatigue with high safety.
28419963	7	65	from	activities	949:958	arg1	mice					1003:1006	the PCS treated mice	987:1006	the PCS treated mice	987:1006	PCS can significantly prolong the duration of the swimming time to exhaustion in mice, decrease BUN, LA levels, increase LDH activities, and the contents of HG in the PCS treated mice.
28419963	2	66	theme	water	215:219	arg1	method					254:259	water extracting-alcohol precipitating method	215:259	water extracting-alcohol precipitating method	215:259	PCS was prepared by water extracting-alcohol precipitating method, and its chemical compositions of monosaccharide were analyzed.
28419963	5	67	theme	food	643:646	arg1	consumption					648:658	food consumption	643:658	food consumption	643:658	No mortality and general symptoms of toxicity were observed in the PCS treated mice (7.5, 15, and 20g/kg body weight), the body weight and food consumption were not significantly changed compared with the normal control group.
28419963	6	68	theme	indicators	783:792	arg1	weights					744:750	The relative weights	731:750	The relative weights of main organ, and biochemical indicators	731:792	The relative weights of main organ, and biochemical indicators also did not markedly change.
28433170	2	0	theme	ear	521:523	arg1	swelling					525:532	delayed-type ear swelling	508:532	delayed-type ear swelling	508:532	The immunological activity test in vivo showed that GLSWA-I could significantly promote dinitrochlorobenzene (DNCB) induced delayed-type ear swelling in mice.
28433170	1	1	theme	aqueous	248:254	arg1	extract					256:262	the aqueous extract	244:262	the aqueous extract of the broken cellular wall Ganoderma lucidum spores	244:315	A water soluble β-glucan (GLSWA-I) with a weight average molecular weight ∼1.57×105g/mol, isolated from the aqueous extract of the broken cellular wall Ganoderma lucidum spores, was purified by anion-exchange and gel-permeation chromatography.
28433170	0	2	theme	β-glucan	93:100	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and immuno-enhancing activity of a highly branched water-soluble β-glucan from the spores of Ganoderma lucidum.
28433170	0	2	theme	β-glucan	93:100	arg1	activity					49:56	immuno-enhancing activity	32:56	immuno-enhancing activity	32:56	Structural characterization and immuno-enhancing activity of a highly branched water-soluble β-glucan from the spores of Ganoderma lucidum.
28433170	1	3	theme	anion-exchange	334:347	arg1	chromatography					368:381	anion-exchange and gel-permeation chromatography	334:381	chromatography	368:381	A water soluble β-glucan (GLSWA-I) with a weight average molecular weight ∼1.57×105g/mol, isolated from the aqueous extract of the broken cellular wall Ganoderma lucidum spores, was purified by anion-exchange and gel-permeation chromatography.
28433170	3	4	theme	polysaccharide	670:683	arg1	GLSWA-I					685:691	polysaccharide GLSWA-I	670:691	polysaccharide GLSWA-I	670:691	Based on the monosaccharides composition analysis, methylation analysis, IR, 1D and 2D NMR spectroscopy, the repeating unit of polysaccharide GLSWA-I was elucidated as follows.
28433170	3	5	theme	composition	572:582	arg1	analysis					584:591	the monosaccharides composition analysis	552:591	the monosaccharides composition analysis	552:591	Based on the monosaccharides composition analysis, methylation analysis, IR, 1D and 2D NMR spectroscopy, the repeating unit of polysaccharide GLSWA-I was elucidated as follows.
28433170	1	6	theme	gel-permeation	353:366	arg1	chromatography					368:381	anion-exchange and gel-permeation chromatography	334:381	chromatography	368:381	A water soluble β-glucan (GLSWA-I) with a weight average molecular weight ∼1.57×105g/mol, isolated from the aqueous extract of the broken cellular wall Ganoderma lucidum spores, was purified by anion-exchange and gel-permeation chromatography.
28433170	1	7	with	β-glucan	156:163	arg1	∼1.57×105g/mol					214:227	a weight average molecular weight ∼1.57×105g/mol	180:227	a weight average molecular weight ∼1.57×105g/mol	180:227	A water soluble β-glucan (GLSWA-I) with a weight average molecular weight ∼1.57×105g/mol, isolated from the aqueous extract of the broken cellular wall Ganoderma lucidum spores, was purified by anion-exchange and gel-permeation chromatography.
28433170	0	8	theme	Ganoderma	121:129	arg1	lucidum					131:137	Ganoderma lucidum	121:137	Ganoderma lucidum	121:137	Structural characterization and immuno-enhancing activity of a highly branched water-soluble β-glucan from the spores of Ganoderma lucidum.
28433170	2	9	theme	delayed-type	508:519	arg1	swelling					525:532	delayed-type ear swelling	508:532	delayed-type ear swelling	508:532	The immunological activity test in vivo showed that GLSWA-I could significantly promote dinitrochlorobenzene (DNCB) induced delayed-type ear swelling in mice.
28433170	0	10	theme	Structural	0:9	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and immuno-enhancing activity of a highly branched water-soluble β-glucan from the spores of Ganoderma lucidum.
28433170	0	11	from	activity	49:56	arg1	spores					111:116	the spores	107:116	the spores of Ganoderma lucidum	107:137	Structural characterization and immuno-enhancing activity of a highly branched water-soluble β-glucan from the spores of Ganoderma lucidum.
28433170	2	12	theme	activity	402:409	arg1	test					411:414	The immunological activity test	384:414	The immunological activity test in vivo	384:422	The immunological activity test in vivo showed that GLSWA-I could significantly promote dinitrochlorobenzene (DNCB) induced delayed-type ear swelling in mice.
28433170	0	13	from	characterization	11:26	arg1	spores					111:116	the spores	107:116	the spores of Ganoderma lucidum	107:137	Structural characterization and immuno-enhancing activity of a highly branched water-soluble β-glucan from the spores of Ganoderma lucidum.
28433170	1	14	theme	broken	271:276	arg1	spores					310:315	the broken cellular wall Ganoderma lucidum spores	267:315	the broken cellular wall Ganoderma lucidum spores	267:315	A water soluble β-glucan (GLSWA-I) with a weight average molecular weight ∼1.57×105g/mol, isolated from the aqueous extract of the broken cellular wall Ganoderma lucidum spores, was purified by anion-exchange and gel-permeation chromatography.
28433170	1	15	theme	weight	182:187	arg1	weight					207:212	a weight average molecular weight	180:212	a weight average molecular weight ∼1.57×105g/mol	180:227	A water soluble β-glucan (GLSWA-I) with a weight average molecular weight ∼1.57×105g/mol, isolated from the aqueous extract of the broken cellular wall Ganoderma lucidum spores, was purified by anion-exchange and gel-permeation chromatography.
28433170	0	16	theme	immuno-enhancing	32:47	arg1	activity					49:56	immuno-enhancing activity	32:56	immuno-enhancing activity	32:56	Structural characterization and immuno-enhancing activity of a highly branched water-soluble β-glucan from the spores of Ganoderma lucidum.
28433170	1	17	theme	cellular	278:285	arg1	lucidum					302:308	cellular wall Ganoderma lucidum	278:308	the broken cellular wall Ganoderma lucidum spores	267:315	A water soluble β-glucan (GLSWA-I) with a weight average molecular weight ∼1.57×105g/mol, isolated from the aqueous extract of the broken cellular wall Ganoderma lucidum spores, was purified by anion-exchange and gel-permeation chromatography.
28433170	1	18	theme	average	189:195	arg1	weight					207:212	a weight average molecular weight	180:212	a weight average molecular weight ∼1.57×105g/mol	180:227	A water soluble β-glucan (GLSWA-I) with a weight average molecular weight ∼1.57×105g/mol, isolated from the aqueous extract of the broken cellular wall Ganoderma lucidum spores, was purified by anion-exchange and gel-permeation chromatography.
28433170	1	19	theme	wall	287:290	arg1	lucidum					302:308	cellular wall Ganoderma lucidum	278:308	the broken cellular wall Ganoderma lucidum spores	267:315	A water soluble β-glucan (GLSWA-I) with a weight average molecular weight ∼1.57×105g/mol, isolated from the aqueous extract of the broken cellular wall Ganoderma lucidum spores, was purified by anion-exchange and gel-permeation chromatography.
28433170	0	20	theme	lucidum	131:137	arg1	spores					111:116	the spores	107:116	the spores of Ganoderma lucidum	107:137	Structural characterization and immuno-enhancing activity of a highly branched water-soluble β-glucan from the spores of Ganoderma lucidum.
28433170	3	21	theme	methylation	594:604	arg1	analysis					606:613	methylation analysis	594:613	methylation analysis	594:613	Based on the monosaccharides composition analysis, methylation analysis, IR, 1D and 2D NMR spectroscopy, the repeating unit of polysaccharide GLSWA-I was elucidated as follows.
28433170	1	22	theme	molecular	197:205	arg1	weight					207:212	a weight average molecular weight	180:212	a weight average molecular weight ∼1.57×105g/mol	180:227	A water soluble β-glucan (GLSWA-I) with a weight average molecular weight ∼1.57×105g/mol, isolated from the aqueous extract of the broken cellular wall Ganoderma lucidum spores, was purified by anion-exchange and gel-permeation chromatography.
28433170	1	23	theme	Ganoderma	292:300	arg1	lucidum					302:308	cellular wall Ganoderma lucidum	278:308	the broken cellular wall Ganoderma lucidum spores	267:315	A water soluble β-glucan (GLSWA-I) with a weight average molecular weight ∼1.57×105g/mol, isolated from the aqueous extract of the broken cellular wall Ganoderma lucidum spores, was purified by anion-exchange and gel-permeation chromatography.
28433170	1	24	attach	isolated	230:237	arg2	GLSWA-I					166:172	GLSWA-I	166:172	GLSWA-I	166:172	A water soluble β-glucan (GLSWA-I) with a weight average molecular weight ∼1.57×105g/mol, isolated from the aqueous extract of the broken cellular wall Ganoderma lucidum spores, was purified by anion-exchange and gel-permeation chromatography.
28433170	1	24	attach	isolated	230:237	arg1	extract					256:262	the aqueous extract	244:262	the aqueous extract of the broken cellular wall Ganoderma lucidum spores	244:315	A water soluble β-glucan (GLSWA-I) with a weight average molecular weight ∼1.57×105g/mol, isolated from the aqueous extract of the broken cellular wall Ganoderma lucidum spores, was purified by anion-exchange and gel-permeation chromatography.
28433170	1	24	attach	isolated	230:237	arg2	β-glucan					156:163	A water soluble β-glucan	140:163	A water soluble β-glucan (GLSWA-I)	140:173	A water soluble β-glucan (GLSWA-I) with a weight average molecular weight ∼1.57×105g/mol, isolated from the aqueous extract of the broken cellular wall Ganoderma lucidum spores, was purified by anion-exchange and gel-permeation chromatography.
28433170	2	25	theme	immunological	388:400	arg1	test					411:414	The immunological activity test	384:414	The immunological activity test in vivo	384:422	The immunological activity test in vivo showed that GLSWA-I could significantly promote dinitrochlorobenzene (DNCB) induced delayed-type ear swelling in mice.
28433170	1	26	theme	weight	207:212	arg1	∼1.57×105g/mol					214:227	a weight average molecular weight ∼1.57×105g/mol	180:227	a weight average molecular weight ∼1.57×105g/mol	180:227	A water soluble β-glucan (GLSWA-I) with a weight average molecular weight ∼1.57×105g/mol, isolated from the aqueous extract of the broken cellular wall Ganoderma lucidum spores, was purified by anion-exchange and gel-permeation chromatography.
28433170	1	27	theme	lucidum	302:308	arg1	spores					310:315	the broken cellular wall Ganoderma lucidum spores	267:315	the broken cellular wall Ganoderma lucidum spores	267:315	A water soluble β-glucan (GLSWA-I) with a weight average molecular weight ∼1.57×105g/mol, isolated from the aqueous extract of the broken cellular wall Ganoderma lucidum spores, was purified by anion-exchange and gel-permeation chromatography.
28433170	1	28	theme	spores	310:315	arg1	extract					256:262	the aqueous extract	244:262	the aqueous extract of the broken cellular wall Ganoderma lucidum spores	244:315	A water soluble β-glucan (GLSWA-I) with a weight average molecular weight ∼1.57×105g/mol, isolated from the aqueous extract of the broken cellular wall Ganoderma lucidum spores, was purified by anion-exchange and gel-permeation chromatography.
28433170	3	29	theme	repeating	652:660	arg1	unit					662:665	the repeating unit	648:665	the repeating unit of polysaccharide GLSWA-I	648:691	Based on the monosaccharides composition analysis, methylation analysis, IR, 1D and 2D NMR spectroscopy, the repeating unit of polysaccharide GLSWA-I was elucidated as follows.
28433170	3	29	theme	repeating	652:660	arg1	follows					711:717	follows	711:717	follows	711:717	Based on the monosaccharides composition analysis, methylation analysis, IR, 1D and 2D NMR spectroscopy, the repeating unit of polysaccharide GLSWA-I was elucidated as follows.
28433170	3	30	theme	monosaccharides	556:570	arg1	analysis					584:591	the monosaccharides composition analysis	552:591	the monosaccharides composition analysis	552:591	Based on the monosaccharides composition analysis, methylation analysis, IR, 1D and 2D NMR spectroscopy, the repeating unit of polysaccharide GLSWA-I was elucidated as follows.
28433170	0	31	from	spores	111:116	arg1	β-glucan					93:100	a highly branched water-soluble β-glucan	61:100	a highly branched water-soluble β-glucan from the spores of Ganoderma lucidum	61:137	Structural characterization and immuno-enhancing activity of a highly branched water-soluble β-glucan from the spores of Ganoderma lucidum.
28433170	0	31	from	spores	111:116	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and immuno-enhancing activity of a highly branched water-soluble β-glucan from the spores of Ganoderma lucidum.
28433170	0	31	from	spores	111:116	arg1	activity					49:56	immuno-enhancing activity	32:56	immuno-enhancing activity	32:56	Structural characterization and immuno-enhancing activity of a highly branched water-soluble β-glucan from the spores of Ganoderma lucidum.
28433170	0	32	theme	water-soluble	79:91	arg1	β-glucan					93:100	a highly branched water-soluble β-glucan	61:100	a highly branched water-soluble β-glucan from the spores of Ganoderma lucidum	61:137	Structural characterization and immuno-enhancing activity of a highly branched water-soluble β-glucan from the spores of Ganoderma lucidum.
28433170	3	33	theme	GLSWA-I	685:691	arg1	unit					662:665	the repeating unit	648:665	the repeating unit of polysaccharide GLSWA-I	648:691	Based on the monosaccharides composition analysis, methylation analysis, IR, 1D and 2D NMR spectroscopy, the repeating unit of polysaccharide GLSWA-I was elucidated as follows.
28433170	3	33	theme	GLSWA-I	685:691	arg1	follows					711:717	follows	711:717	follows	711:717	Based on the monosaccharides composition analysis, methylation analysis, IR, 1D and 2D NMR spectroscopy, the repeating unit of polysaccharide GLSWA-I was elucidated as follows.
28433170	3	34	theme	2D	627:628	arg1	spectroscopy					634:645	2D NMR spectroscopy	627:645	2D NMR spectroscopy	627:645	Based on the monosaccharides composition analysis, methylation analysis, IR, 1D and 2D NMR spectroscopy, the repeating unit of polysaccharide GLSWA-I was elucidated as follows.
28433170	1	35	theme	soluble	148:154	arg1	β-glucan					156:163	A water soluble β-glucan	140:163	A water soluble β-glucan (GLSWA-I)	140:173	A water soluble β-glucan (GLSWA-I) with a weight average molecular weight ∼1.57×105g/mol, isolated from the aqueous extract of the broken cellular wall Ganoderma lucidum spores, was purified by anion-exchange and gel-permeation chromatography.
28433170	1	35	theme	soluble	148:154	arg1	GLSWA-I					166:172	GLSWA-I	166:172	GLSWA-I	166:172	A water soluble β-glucan (GLSWA-I) with a weight average molecular weight ∼1.57×105g/mol, isolated from the aqueous extract of the broken cellular wall Ganoderma lucidum spores, was purified by anion-exchange and gel-permeation chromatography.
28433170	0	36	theme	branched	70:77	arg1	β-glucan					93:100	a highly branched water-soluble β-glucan	61:100	a highly branched water-soluble β-glucan from the spores of Ganoderma lucidum	61:137	Structural characterization and immuno-enhancing activity of a highly branched water-soluble β-glucan from the spores of Ganoderma lucidum.
28433170	3	37	theme	NMR	630:632	arg1	spectroscopy					634:645	2D NMR spectroscopy	627:645	2D NMR spectroscopy	627:645	Based on the monosaccharides composition analysis, methylation analysis, IR, 1D and 2D NMR spectroscopy, the repeating unit of polysaccharide GLSWA-I was elucidated as follows.
26344261	2	0	theme	anti-tumor	518:527	arg1	activities					529:538	differing anti-tumor activities	508:538	differing anti-tumor activities	508:538	The mole percentages of three major EPS monosaccharides, glucose, galactose and mannose, varied depending on culture conditions and the resulting EPS displayed differing anti-tumor activities.
26344261	3	1	theme	EPS	1012:1014	arg1	monosaccharides					1016:1030	G. lucidum EPS monosaccharides	1001:1030	G. lucidum EPS monosaccharides	1001:1030	In nine tested enzymes, higher enzyme activities were correlated with higher temperature and lower initial pH. Altered mole percentages of galactose and mannose under various culture conditions were associated with activities of α-phosphoglucomutase (PGM) and phosphoglucose isomerase (PGI), respectively, and that of mannose was also associated with phosphomannose isomerase (PMI) activity only under various pH. Our findings suggest that mole percentages of G. lucidum EPS monosaccharides can be manipulated by changes of culture conditions that affect enzyme activities, and that novel fermentation strategies based on this approach may enhance production and biological activity of EPS.
26344261	2	2	theme	EPS	384:386	arg1	galactose					414:422	galactose	414:422	galactose	414:422	The mole percentages of three major EPS monosaccharides, glucose, galactose and mannose, varied depending on culture conditions and the resulting EPS displayed differing anti-tumor activities.
26344261	2	2	theme	EPS	384:386	arg1	glucose					405:411	glucose	405:411	glucose	405:411	The mole percentages of three major EPS monosaccharides, glucose, galactose and mannose, varied depending on culture conditions and the resulting EPS displayed differing anti-tumor activities.
26344261	2	2	theme	EPS	384:386	arg1	monosaccharides					388:402	three major EPS monosaccharides	372:402	three major EPS monosaccharides	372:402	The mole percentages of three major EPS monosaccharides, glucose, galactose and mannose, varied depending on culture conditions and the resulting EPS displayed differing anti-tumor activities.
26344261	2	2	theme	EPS	384:386	arg1	mannose					428:434	mannose	428:434	mannose	428:434	The mole percentages of three major EPS monosaccharides, glucose, galactose and mannose, varied depending on culture conditions and the resulting EPS displayed differing anti-tumor activities.
26344261	1	3	theme	synthesis	272:280	arg1	lucidum					218:224	Ganoderma lucidum exopolysaccharide (EPS)	208:248	Ganoderma lucidum exopolysaccharide (EPS)	208:248	We investigated the relationship between monosaccharide composition of Ganoderma lucidum exopolysaccharide (EPS) and activities of EPS synthesis enzymes under various culture temperatures and initial pH values.
26344261	1	3	theme	synthesis	272:280	arg1	activities					254:263	activities	254:263	activities of EPS synthesis	254:280	We investigated the relationship between monosaccharide composition of Ganoderma lucidum exopolysaccharide (EPS) and activities of EPS synthesis enzymes under various culture temperatures and initial pH values.
26344261	0	4	theme	exopolysaccharide	81:97	arg1	composition					48:58	monosaccharide composition	33:58	monosaccharide composition of Ganoderma lucidum exopolysaccharide	33:97	Effects of culture conditions on monosaccharide composition of Ganoderma lucidum exopolysaccharide and on activities of related enzymes.
26344261	1	5	theme	monosaccharide	178:191	arg1	composition					193:203	monosaccharide composition	178:203	monosaccharide composition of Ganoderma lucidum exopolysaccharide (EPS) and activities of EPS synthesis enzymes	178:288	We investigated the relationship between monosaccharide composition of Ganoderma lucidum exopolysaccharide (EPS) and activities of EPS synthesis enzymes under various culture temperatures and initial pH values.
26344261	1	6	theme	enzymes	282:288	arg1	composition					193:203	monosaccharide composition	178:203	monosaccharide composition of Ganoderma lucidum exopolysaccharide (EPS) and activities of EPS synthesis enzymes	178:288	We investigated the relationship between monosaccharide composition of Ganoderma lucidum exopolysaccharide (EPS) and activities of EPS synthesis enzymes under various culture temperatures and initial pH values.
26344261	0	7	from	Effects	0:6	arg1	activities					106:115	activities	106:115	activities of related enzymes	106:134	Effects of culture conditions on monosaccharide composition of Ganoderma lucidum exopolysaccharide and on activities of related enzymes.
26344261	0	7	from	Effects	0:6	arg1	composition					48:58	monosaccharide composition	33:58	monosaccharide composition of Ganoderma lucidum exopolysaccharide	33:97	Effects of culture conditions on monosaccharide composition of Ganoderma lucidum exopolysaccharide and on activities of related enzymes.
26344261	3	8	theme	biological	1204:1213	arg1	activity					1215:1222	biological activity	1204:1222	biological activity	1204:1222	In nine tested enzymes, higher enzyme activities were correlated with higher temperature and lower initial pH. Altered mole percentages of galactose and mannose under various culture conditions were associated with activities of α-phosphoglucomutase (PGM) and phosphoglucose isomerase (PGI), respectively, and that of mannose was also associated with phosphomannose isomerase (PMI) activity only under various pH. Our findings suggest that mole percentages of G. lucidum EPS monosaccharides can be manipulated by changes of culture conditions that affect enzyme activities, and that novel fermentation strategies based on this approach may enhance production and biological activity of EPS.
26344261	3	9	theme	enzyme	572:577	arg1	activities					579:588	higher enzyme activities	565:588	higher enzyme activities	565:588	In nine tested enzymes, higher enzyme activities were correlated with higher temperature and lower initial pH. Altered mole percentages of galactose and mannose under various culture conditions were associated with activities of α-phosphoglucomutase (PGM) and phosphoglucose isomerase (PGI), respectively, and that of mannose was also associated with phosphomannose isomerase (PMI) activity only under various pH. Our findings suggest that mole percentages of G. lucidum EPS monosaccharides can be manipulated by changes of culture conditions that affect enzyme activities, and that novel fermentation strategies based on this approach may enhance production and biological activity of EPS.
26344261	3	10	theme	enzyme	1096:1101	arg1	activities					1103:1112	enzyme activities	1096:1112	enzyme activities	1096:1112	In nine tested enzymes, higher enzyme activities were correlated with higher temperature and lower initial pH. Altered mole percentages of galactose and mannose under various culture conditions were associated with activities of α-phosphoglucomutase (PGM) and phosphoglucose isomerase (PGI), respectively, and that of mannose was also associated with phosphomannose isomerase (PMI) activity only under various pH. Our findings suggest that mole percentages of G. lucidum EPS monosaccharides can be manipulated by changes of culture conditions that affect enzyme activities, and that novel fermentation strategies based on this approach may enhance production and biological activity of EPS.
26344261	3	11	theme	α-phosphoglucomutase	770:789	arg1	activities					756:765	activities	756:765	activities of α-phosphoglucomutase (PGM) and phosphoglucose isomerase (PGI), respectively	756:844	In nine tested enzymes, higher enzyme activities were correlated with higher temperature and lower initial pH. Altered mole percentages of galactose and mannose under various culture conditions were associated with activities of α-phosphoglucomutase (PGM) and phosphoglucose isomerase (PGI), respectively, and that of mannose was also associated with phosphomannose isomerase (PMI) activity only under various pH. Our findings suggest that mole percentages of G. lucidum EPS monosaccharides can be manipulated by changes of culture conditions that affect enzyme activities, and that novel fermentation strategies based on this approach may enhance production and biological activity of EPS.
26344261	3	12	theme	various	943:949	arg1	findings					959:966	various pH. Our findings	943:966	various pH. Our findings	943:966	In nine tested enzymes, higher enzyme activities were correlated with higher temperature and lower initial pH. Altered mole percentages of galactose and mannose under various culture conditions were associated with activities of α-phosphoglucomutase (PGM) and phosphoglucose isomerase (PGI), respectively, and that of mannose was also associated with phosphomannose isomerase (PMI) activity only under various pH. Our findings suggest that mole percentages of G. lucidum EPS monosaccharides can be manipulated by changes of culture conditions that affect enzyme activities, and that novel fermentation strategies based on this approach may enhance production and biological activity of EPS.
26344261	1	13	theme	various	296:302	arg1	temperatures					312:323	various culture temperatures	296:323	various culture temperatures	296:323	We investigated the relationship between monosaccharide composition of Ganoderma lucidum exopolysaccharide (EPS) and activities of EPS synthesis enzymes under various culture temperatures and initial pH values.
26344261	0	14	theme	related	120:126	arg1	enzymes					128:134	related enzymes	120:134	related enzymes	120:134	Effects of culture conditions on monosaccharide composition of Ganoderma lucidum exopolysaccharide and on activities of related enzymes.
26344261	3	15	theme	novel	1124:1128	arg1	strategies					1143:1152	novel fermentation strategies	1124:1152	novel fermentation strategies based on this approach	1124:1175	In nine tested enzymes, higher enzyme activities were correlated with higher temperature and lower initial pH. Altered mole percentages of galactose and mannose under various culture conditions were associated with activities of α-phosphoglucomutase (PGM) and phosphoglucose isomerase (PGI), respectively, and that of mannose was also associated with phosphomannose isomerase (PMI) activity only under various pH. Our findings suggest that mole percentages of G. lucidum EPS monosaccharides can be manipulated by changes of culture conditions that affect enzyme activities, and that novel fermentation strategies based on this approach may enhance production and biological activity of EPS.
26344261	3	16	theme	pH.	951:953	arg1	findings					959:966	various pH. Our findings	943:966	various pH. Our findings	943:966	In nine tested enzymes, higher enzyme activities were correlated with higher temperature and lower initial pH. Altered mole percentages of galactose and mannose under various culture conditions were associated with activities of α-phosphoglucomutase (PGM) and phosphoglucose isomerase (PGI), respectively, and that of mannose was also associated with phosphomannose isomerase (PMI) activity only under various pH. Our findings suggest that mole percentages of G. lucidum EPS monosaccharides can be manipulated by changes of culture conditions that affect enzyme activities, and that novel fermentation strategies based on this approach may enhance production and biological activity of EPS.
26344261	3	17	theme	fermentation	1130:1141	arg1	strategies					1143:1152	novel fermentation strategies	1124:1152	novel fermentation strategies based on this approach	1124:1175	In nine tested enzymes, higher enzyme activities were correlated with higher temperature and lower initial pH. Altered mole percentages of galactose and mannose under various culture conditions were associated with activities of α-phosphoglucomutase (PGM) and phosphoglucose isomerase (PGI), respectively, and that of mannose was also associated with phosphomannose isomerase (PMI) activity only under various pH. Our findings suggest that mole percentages of G. lucidum EPS monosaccharides can be manipulated by changes of culture conditions that affect enzyme activities, and that novel fermentation strategies based on this approach may enhance production and biological activity of EPS.
26344261	3	18	theme	conditions	1073:1082	arg1	changes					1054:1060	changes	1054:1060	changes of culture conditions that affect enzyme activities, and that novel fermentation strategies based on this approach may enhance production and biological activity of EPS	1054:1229	In nine tested enzymes, higher enzyme activities were correlated with higher temperature and lower initial pH. Altered mole percentages of galactose and mannose under various culture conditions were associated with activities of α-phosphoglucomutase (PGM) and phosphoglucose isomerase (PGI), respectively, and that of mannose was also associated with phosphomannose isomerase (PMI) activity only under various pH. Our findings suggest that mole percentages of G. lucidum EPS monosaccharides can be manipulated by changes of culture conditions that affect enzyme activities, and that novel fermentation strategies based on this approach may enhance production and biological activity of EPS.
26344261	3	19	theme	higher	565:570	arg1	activities					579:588	higher enzyme activities	565:588	higher enzyme activities	565:588	In nine tested enzymes, higher enzyme activities were correlated with higher temperature and lower initial pH. Altered mole percentages of galactose and mannose under various culture conditions were associated with activities of α-phosphoglucomutase (PGM) and phosphoglucose isomerase (PGI), respectively, and that of mannose was also associated with phosphomannose isomerase (PMI) activity only under various pH. Our findings suggest that mole percentages of G. lucidum EPS monosaccharides can be manipulated by changes of culture conditions that affect enzyme activities, and that novel fermentation strategies based on this approach may enhance production and biological activity of EPS.
26344261	3	20	theme	EPS	1227:1229	arg1	activity					1215:1222	biological activity	1204:1222	biological activity	1204:1222	In nine tested enzymes, higher enzyme activities were correlated with higher temperature and lower initial pH. Altered mole percentages of galactose and mannose under various culture conditions were associated with activities of α-phosphoglucomutase (PGM) and phosphoglucose isomerase (PGI), respectively, and that of mannose was also associated with phosphomannose isomerase (PMI) activity only under various pH. Our findings suggest that mole percentages of G. lucidum EPS monosaccharides can be manipulated by changes of culture conditions that affect enzyme activities, and that novel fermentation strategies based on this approach may enhance production and biological activity of EPS.
26344261	3	20	theme	EPS	1227:1229	arg1	production					1189:1198	production	1189:1198	production	1189:1198	In nine tested enzymes, higher enzyme activities were correlated with higher temperature and lower initial pH. Altered mole percentages of galactose and mannose under various culture conditions were associated with activities of α-phosphoglucomutase (PGM) and phosphoglucose isomerase (PGI), respectively, and that of mannose was also associated with phosphomannose isomerase (PMI) activity only under various pH. Our findings suggest that mole percentages of G. lucidum EPS monosaccharides can be manipulated by changes of culture conditions that affect enzyme activities, and that novel fermentation strategies based on this approach may enhance production and biological activity of EPS.
26344261	2	21	theme	differing	508:516	arg1	activities					529:538	differing anti-tumor activities	508:538	differing anti-tumor activities	508:538	The mole percentages of three major EPS monosaccharides, glucose, galactose and mannose, varied depending on culture conditions and the resulting EPS displayed differing anti-tumor activities.
26344261	3	22	theme	phosphoglucose	801:814	arg1	PGI					827:829	PGI	827:829	PGI	827:829	In nine tested enzymes, higher enzyme activities were correlated with higher temperature and lower initial pH. Altered mole percentages of galactose and mannose under various culture conditions were associated with activities of α-phosphoglucomutase (PGM) and phosphoglucose isomerase (PGI), respectively, and that of mannose was also associated with phosphomannose isomerase (PMI) activity only under various pH. Our findings suggest that mole percentages of G. lucidum EPS monosaccharides can be manipulated by changes of culture conditions that affect enzyme activities, and that novel fermentation strategies based on this approach may enhance production and biological activity of EPS.
26344261	3	22	theme	phosphoglucose	801:814	arg1	isomerase					816:824	phosphoglucose isomerase	801:824	phosphoglucose isomerase (PGI)	801:830	In nine tested enzymes, higher enzyme activities were correlated with higher temperature and lower initial pH. Altered mole percentages of galactose and mannose under various culture conditions were associated with activities of α-phosphoglucomutase (PGM) and phosphoglucose isomerase (PGI), respectively, and that of mannose was also associated with phosphomannose isomerase (PMI) activity only under various pH. Our findings suggest that mole percentages of G. lucidum EPS monosaccharides can be manipulated by changes of culture conditions that affect enzyme activities, and that novel fermentation strategies based on this approach may enhance production and biological activity of EPS.
26344261	1	23	theme	Ganoderma	208:216	arg1	lucidum					218:224	Ganoderma lucidum exopolysaccharide (EPS)	208:248	Ganoderma lucidum exopolysaccharide (EPS)	208:248	We investigated the relationship between monosaccharide composition of Ganoderma lucidum exopolysaccharide (EPS) and activities of EPS synthesis enzymes under various culture temperatures and initial pH values.
26344261	1	24	theme	culture	304:310	arg1	temperatures					312:323	various culture temperatures	296:323	various culture temperatures	296:323	We investigated the relationship between monosaccharide composition of Ganoderma lucidum exopolysaccharide (EPS) and activities of EPS synthesis enzymes under various culture temperatures and initial pH values.
26344261	3	25	theme	higher	611:616	arg1	temperature					618:628	higher temperature	611:628	higher temperature	611:628	In nine tested enzymes, higher enzyme activities were correlated with higher temperature and lower initial pH. Altered mole percentages of galactose and mannose under various culture conditions were associated with activities of α-phosphoglucomutase (PGM) and phosphoglucose isomerase (PGI), respectively, and that of mannose was also associated with phosphomannose isomerase (PMI) activity only under various pH. Our findings suggest that mole percentages of G. lucidum EPS monosaccharides can be manipulated by changes of culture conditions that affect enzyme activities, and that novel fermentation strategies based on this approach may enhance production and biological activity of EPS.
26344261	1	26	theme	lucidum	218:224	arg1	enzymes					282:288	Ganoderma lucidum exopolysaccharide (EPS) and activities of EPS synthesis enzymes	208:288	Ganoderma lucidum exopolysaccharide (EPS) and activities of EPS synthesis enzymes	208:288	We investigated the relationship between monosaccharide composition of Ganoderma lucidum exopolysaccharide (EPS) and activities of EPS synthesis enzymes under various culture temperatures and initial pH values.
26344261	0	27	theme	conditions	19:28	arg1	Effects					0:6	Effects	0:6	Effects of culture conditions on monosaccharide composition of Ganoderma lucidum exopolysaccharide and on activities of related enzymes	0:134	Effects of culture conditions on monosaccharide composition of Ganoderma lucidum exopolysaccharide and on activities of related enzymes.
26344261	3	28	theme	isomerase	816:824	arg1	activities					756:765	activities	756:765	activities of α-phosphoglucomutase (PGM) and phosphoglucose isomerase (PGI), respectively	756:844	In nine tested enzymes, higher enzyme activities were correlated with higher temperature and lower initial pH. Altered mole percentages of galactose and mannose under various culture conditions were associated with activities of α-phosphoglucomutase (PGM) and phosphoglucose isomerase (PGI), respectively, and that of mannose was also associated with phosphomannose isomerase (PMI) activity only under various pH. Our findings suggest that mole percentages of G. lucidum EPS monosaccharides can be manipulated by changes of culture conditions that affect enzyme activities, and that novel fermentation strategies based on this approach may enhance production and biological activity of EPS.
26344261	3	29	theme	culture	1065:1071	arg1	conditions					1073:1082	culture conditions	1065:1082	culture conditions that affect enzyme activities, and that novel fermentation strategies based on this approach may enhance production and biological activity of EPS	1065:1229	In nine tested enzymes, higher enzyme activities were correlated with higher temperature and lower initial pH. Altered mole percentages of galactose and mannose under various culture conditions were associated with activities of α-phosphoglucomutase (PGM) and phosphoglucose isomerase (PGI), respectively, and that of mannose was also associated with phosphomannose isomerase (PMI) activity only under various pH. Our findings suggest that mole percentages of G. lucidum EPS monosaccharides can be manipulated by changes of culture conditions that affect enzyme activities, and that novel fermentation strategies based on this approach may enhance production and biological activity of EPS.
26344261	0	30	theme	culture	11:17	arg1	conditions					19:28	culture conditions	11:28	culture conditions	11:28	Effects of culture conditions on monosaccharide composition of Ganoderma lucidum exopolysaccharide and on activities of related enzymes.
26344261	0	31	theme	enzymes	128:134	arg1	activities					106:115	activities	106:115	activities of related enzymes	106:134	Effects of culture conditions on monosaccharide composition of Ganoderma lucidum exopolysaccharide and on activities of related enzymes.
26344261	2	32	theme	major	378:382	arg1	galactose					414:422	galactose	414:422	galactose	414:422	The mole percentages of three major EPS monosaccharides, glucose, galactose and mannose, varied depending on culture conditions and the resulting EPS displayed differing anti-tumor activities.
26344261	2	32	theme	major	378:382	arg1	glucose					405:411	glucose	405:411	glucose	405:411	The mole percentages of three major EPS monosaccharides, glucose, galactose and mannose, varied depending on culture conditions and the resulting EPS displayed differing anti-tumor activities.
26344261	2	32	theme	major	378:382	arg1	monosaccharides					388:402	three major EPS monosaccharides	372:402	three major EPS monosaccharides	372:402	The mole percentages of three major EPS monosaccharides, glucose, galactose and mannose, varied depending on culture conditions and the resulting EPS displayed differing anti-tumor activities.
26344261	2	32	theme	major	378:382	arg1	mannose					428:434	mannose	428:434	mannose	428:434	The mole percentages of three major EPS monosaccharides, glucose, galactose and mannose, varied depending on culture conditions and the resulting EPS displayed differing anti-tumor activities.
26344261	0	33	theme	monosaccharide	33:46	arg1	composition					48:58	monosaccharide composition	33:58	monosaccharide composition of Ganoderma lucidum exopolysaccharide	33:97	Effects of culture conditions on monosaccharide composition of Ganoderma lucidum exopolysaccharide and on activities of related enzymes.
26344261	1	34	theme	initial	329:335	arg1	values					340:345	initial pH values	329:345	initial pH values	329:345	We investigated the relationship between monosaccharide composition of Ganoderma lucidum exopolysaccharide (EPS) and activities of EPS synthesis enzymes under various culture temperatures and initial pH values.
26344261	3	35	theme	mannose	694:700	arg1	percentages					665:675	lower initial pH. Altered mole percentages	634:675	lower initial pH. Altered mole percentages of galactose and mannose under various culture conditions	634:733	In nine tested enzymes, higher enzyme activities were correlated with higher temperature and lower initial pH. Altered mole percentages of galactose and mannose under various culture conditions were associated with activities of α-phosphoglucomutase (PGM) and phosphoglucose isomerase (PGI), respectively, and that of mannose was also associated with phosphomannose isomerase (PMI) activity only under various pH. Our findings suggest that mole percentages of G. lucidum EPS monosaccharides can be manipulated by changes of culture conditions that affect enzyme activities, and that novel fermentation strategies based on this approach may enhance production and biological activity of EPS.
26344261	3	36	theme	lower	634:638	arg1	percentages					665:675	lower initial pH. Altered mole percentages	634:675	lower initial pH. Altered mole percentages of galactose and mannose under various culture conditions	634:733	In nine tested enzymes, higher enzyme activities were correlated with higher temperature and lower initial pH. Altered mole percentages of galactose and mannose under various culture conditions were associated with activities of α-phosphoglucomutase (PGM) and phosphoglucose isomerase (PGI), respectively, and that of mannose was also associated with phosphomannose isomerase (PMI) activity only under various pH. Our findings suggest that mole percentages of G. lucidum EPS monosaccharides can be manipulated by changes of culture conditions that affect enzyme activities, and that novel fermentation strategies based on this approach may enhance production and biological activity of EPS.
26344261	1	37	theme	pH	337:338	arg1	values					340:345	initial pH values	329:345	initial pH values	329:345	We investigated the relationship between monosaccharide composition of Ganoderma lucidum exopolysaccharide (EPS) and activities of EPS synthesis enzymes under various culture temperatures and initial pH values.
26344261	1	38	dep	lucidum	218:224	arg1	EPS					245:247	EPS	245:247	EPS	245:247	We investigated the relationship between monosaccharide composition of Ganoderma lucidum exopolysaccharide (EPS) and activities of EPS synthesis enzymes under various culture temperatures and initial pH values.
26344261	1	38	dep	lucidum	218:224	arg1	exopolysaccharide					226:242	exopolysaccharide	226:242	Ganoderma lucidum exopolysaccharide (EPS)	208:248	We investigated the relationship between monosaccharide composition of Ganoderma lucidum exopolysaccharide (EPS) and activities of EPS synthesis enzymes under various culture temperatures and initial pH values.
26344261	2	39	theme	resulting	484:492	arg1	EPS					494:496	the resulting EPS	480:496	the resulting EPS	480:496	The mole percentages of three major EPS monosaccharides, glucose, galactose and mannose, varied depending on culture conditions and the resulting EPS displayed differing anti-tumor activities.
26344261	3	40	theme	galactose	680:688	arg1	percentages					665:675	lower initial pH. Altered mole percentages	634:675	lower initial pH. Altered mole percentages of galactose and mannose under various culture conditions	634:733	In nine tested enzymes, higher enzyme activities were correlated with higher temperature and lower initial pH. Altered mole percentages of galactose and mannose under various culture conditions were associated with activities of α-phosphoglucomutase (PGM) and phosphoglucose isomerase (PGI), respectively, and that of mannose was also associated with phosphomannose isomerase (PMI) activity only under various pH. Our findings suggest that mole percentages of G. lucidum EPS monosaccharides can be manipulated by changes of culture conditions that affect enzyme activities, and that novel fermentation strategies based on this approach may enhance production and biological activity of EPS.
26344261	3	41	theme	G.	1001:1002	arg1	monosaccharides					1016:1030	G. lucidum EPS monosaccharides	1001:1030	G. lucidum EPS monosaccharides	1001:1030	In nine tested enzymes, higher enzyme activities were correlated with higher temperature and lower initial pH. Altered mole percentages of galactose and mannose under various culture conditions were associated with activities of α-phosphoglucomutase (PGM) and phosphoglucose isomerase (PGI), respectively, and that of mannose was also associated with phosphomannose isomerase (PMI) activity only under various pH. Our findings suggest that mole percentages of G. lucidum EPS monosaccharides can be manipulated by changes of culture conditions that affect enzyme activities, and that novel fermentation strategies based on this approach may enhance production and biological activity of EPS.
26344261	2	42	theme	culture	457:463	arg1	conditions					465:474	culture conditions	457:474	culture conditions	457:474	The mole percentages of three major EPS monosaccharides, glucose, galactose and mannose, varied depending on culture conditions and the resulting EPS displayed differing anti-tumor activities.
26344261	3	43	theme	culture	716:722	arg1	conditions					724:733	various culture conditions	708:733	various culture conditions	708:733	In nine tested enzymes, higher enzyme activities were correlated with higher temperature and lower initial pH. Altered mole percentages of galactose and mannose under various culture conditions were associated with activities of α-phosphoglucomutase (PGM) and phosphoglucose isomerase (PGI), respectively, and that of mannose was also associated with phosphomannose isomerase (PMI) activity only under various pH. Our findings suggest that mole percentages of G. lucidum EPS monosaccharides can be manipulated by changes of culture conditions that affect enzyme activities, and that novel fermentation strategies based on this approach may enhance production and biological activity of EPS.
26344261	3	44	theme	mole	981:984	arg1	percentages					986:996	mole percentages	981:996	mole percentages of G. lucidum EPS monosaccharides	981:1030	In nine tested enzymes, higher enzyme activities were correlated with higher temperature and lower initial pH. Altered mole percentages of galactose and mannose under various culture conditions were associated with activities of α-phosphoglucomutase (PGM) and phosphoglucose isomerase (PGI), respectively, and that of mannose was also associated with phosphomannose isomerase (PMI) activity only under various pH. Our findings suggest that mole percentages of G. lucidum EPS monosaccharides can be manipulated by changes of culture conditions that affect enzyme activities, and that novel fermentation strategies based on this approach may enhance production and biological activity of EPS.
26344261	3	45	theme	phosphomannose	892:905	arg1	PMI					918:920	PMI	918:920	PMI	918:920	In nine tested enzymes, higher enzyme activities were correlated with higher temperature and lower initial pH. Altered mole percentages of galactose and mannose under various culture conditions were associated with activities of α-phosphoglucomutase (PGM) and phosphoglucose isomerase (PGI), respectively, and that of mannose was also associated with phosphomannose isomerase (PMI) activity only under various pH. Our findings suggest that mole percentages of G. lucidum EPS monosaccharides can be manipulated by changes of culture conditions that affect enzyme activities, and that novel fermentation strategies based on this approach may enhance production and biological activity of EPS.
26344261	3	45	theme	phosphomannose	892:905	arg1	isomerase					907:915	phosphomannose isomerase	892:915	phosphomannose isomerase (PMI) activity	892:930	In nine tested enzymes, higher enzyme activities were correlated with higher temperature and lower initial pH. Altered mole percentages of galactose and mannose under various culture conditions were associated with activities of α-phosphoglucomutase (PGM) and phosphoglucose isomerase (PGI), respectively, and that of mannose was also associated with phosphomannose isomerase (PMI) activity only under various pH. Our findings suggest that mole percentages of G. lucidum EPS monosaccharides can be manipulated by changes of culture conditions that affect enzyme activities, and that novel fermentation strategies based on this approach may enhance production and biological activity of EPS.
26344261	3	46	theme	mole	660:663	arg1	percentages					665:675	lower initial pH. Altered mole percentages	634:675	lower initial pH. Altered mole percentages of galactose and mannose under various culture conditions	634:733	In nine tested enzymes, higher enzyme activities were correlated with higher temperature and lower initial pH. Altered mole percentages of galactose and mannose under various culture conditions were associated with activities of α-phosphoglucomutase (PGM) and phosphoglucose isomerase (PGI), respectively, and that of mannose was also associated with phosphomannose isomerase (PMI) activity only under various pH. Our findings suggest that mole percentages of G. lucidum EPS monosaccharides can be manipulated by changes of culture conditions that affect enzyme activities, and that novel fermentation strategies based on this approach may enhance production and biological activity of EPS.
26344261	3	47	theme	initial	640:646	arg1	percentages					665:675	lower initial pH. Altered mole percentages	634:675	lower initial pH. Altered mole percentages of galactose and mannose under various culture conditions	634:733	In nine tested enzymes, higher enzyme activities were correlated with higher temperature and lower initial pH. Altered mole percentages of galactose and mannose under various culture conditions were associated with activities of α-phosphoglucomutase (PGM) and phosphoglucose isomerase (PGI), respectively, and that of mannose was also associated with phosphomannose isomerase (PMI) activity only under various pH. Our findings suggest that mole percentages of G. lucidum EPS monosaccharides can be manipulated by changes of culture conditions that affect enzyme activities, and that novel fermentation strategies based on this approach may enhance production and biological activity of EPS.
26344261	3	48	theme	isomerase	907:915	arg1	activity					923:930	phosphomannose isomerase (PMI) activity	892:930	phosphomannose isomerase (PMI) activity	892:930	In nine tested enzymes, higher enzyme activities were correlated with higher temperature and lower initial pH. Altered mole percentages of galactose and mannose under various culture conditions were associated with activities of α-phosphoglucomutase (PGM) and phosphoglucose isomerase (PGI), respectively, and that of mannose was also associated with phosphomannose isomerase (PMI) activity only under various pH. Our findings suggest that mole percentages of G. lucidum EPS monosaccharides can be manipulated by changes of culture conditions that affect enzyme activities, and that novel fermentation strategies based on this approach may enhance production and biological activity of EPS.
26344261	3	49	theme	monosaccharides	1016:1030	arg1	percentages					986:996	mole percentages	981:996	mole percentages of G. lucidum EPS monosaccharides	981:1030	In nine tested enzymes, higher enzyme activities were correlated with higher temperature and lower initial pH. Altered mole percentages of galactose and mannose under various culture conditions were associated with activities of α-phosphoglucomutase (PGM) and phosphoglucose isomerase (PGI), respectively, and that of mannose was also associated with phosphomannose isomerase (PMI) activity only under various pH. Our findings suggest that mole percentages of G. lucidum EPS monosaccharides can be manipulated by changes of culture conditions that affect enzyme activities, and that novel fermentation strategies based on this approach may enhance production and biological activity of EPS.
26344261	2	50	theme	mole	352:355	arg1	percentages					357:367	The mole percentages	348:367	The mole percentages of three major EPS monosaccharides, glucose, galactose and mannose,	348:435	The mole percentages of three major EPS monosaccharides, glucose, galactose and mannose, varied depending on culture conditions and the resulting EPS displayed differing anti-tumor activities.
26344261	1	51	theme	activities	254:263	arg1	enzymes					282:288	Ganoderma lucidum exopolysaccharide (EPS) and activities of EPS synthesis enzymes	208:288	Ganoderma lucidum exopolysaccharide (EPS) and activities of EPS synthesis enzymes	208:288	We investigated the relationship between monosaccharide composition of Ganoderma lucidum exopolysaccharide (EPS) and activities of EPS synthesis enzymes under various culture temperatures and initial pH values.
26344261	0	52	theme	lucidum	73:79	arg1	exopolysaccharide					81:97	Ganoderma lucidum exopolysaccharide	63:97	Ganoderma lucidum exopolysaccharide	63:97	Effects of culture conditions on monosaccharide composition of Ganoderma lucidum exopolysaccharide and on activities of related enzymes.
26344261	3	53	theme	various	708:714	arg1	conditions					724:733	various culture conditions	708:733	various culture conditions	708:733	In nine tested enzymes, higher enzyme activities were correlated with higher temperature and lower initial pH. Altered mole percentages of galactose and mannose under various culture conditions were associated with activities of α-phosphoglucomutase (PGM) and phosphoglucose isomerase (PGI), respectively, and that of mannose was also associated with phosphomannose isomerase (PMI) activity only under various pH. Our findings suggest that mole percentages of G. lucidum EPS monosaccharides can be manipulated by changes of culture conditions that affect enzyme activities, and that novel fermentation strategies based on this approach may enhance production and biological activity of EPS.
26344261	3	54	theme	pH.	648:650	arg1	percentages					665:675	lower initial pH. Altered mole percentages	634:675	lower initial pH. Altered mole percentages of galactose and mannose under various culture conditions	634:733	In nine tested enzymes, higher enzyme activities were correlated with higher temperature and lower initial pH. Altered mole percentages of galactose and mannose under various culture conditions were associated with activities of α-phosphoglucomutase (PGM) and phosphoglucose isomerase (PGI), respectively, and that of mannose was also associated with phosphomannose isomerase (PMI) activity only under various pH. Our findings suggest that mole percentages of G. lucidum EPS monosaccharides can be manipulated by changes of culture conditions that affect enzyme activities, and that novel fermentation strategies based on this approach may enhance production and biological activity of EPS.
26344261	3	55	theme	tested	549:554	arg1	enzymes					556:562	nine tested enzymes	544:562	nine tested enzymes	544:562	In nine tested enzymes, higher enzyme activities were correlated with higher temperature and lower initial pH. Altered mole percentages of galactose and mannose under various culture conditions were associated with activities of α-phosphoglucomutase (PGM) and phosphoglucose isomerase (PGI), respectively, and that of mannose was also associated with phosphomannose isomerase (PMI) activity only under various pH. Our findings suggest that mole percentages of G. lucidum EPS monosaccharides can be manipulated by changes of culture conditions that affect enzyme activities, and that novel fermentation strategies based on this approach may enhance production and biological activity of EPS.
26344261	0	56	theme	Ganoderma	63:71	arg1	lucidum					73:79	Ganoderma lucidum	63:79	Ganoderma lucidum exopolysaccharide	63:97	Effects of culture conditions on monosaccharide composition of Ganoderma lucidum exopolysaccharide and on activities of related enzymes.
26344261	3	57	theme	Altered	652:658	arg1	percentages					665:675	lower initial pH. Altered mole percentages	634:675	lower initial pH. Altered mole percentages of galactose and mannose under various culture conditions	634:733	In nine tested enzymes, higher enzyme activities were correlated with higher temperature and lower initial pH. Altered mole percentages of galactose and mannose under various culture conditions were associated with activities of α-phosphoglucomutase (PGM) and phosphoglucose isomerase (PGI), respectively, and that of mannose was also associated with phosphomannose isomerase (PMI) activity only under various pH. Our findings suggest that mole percentages of G. lucidum EPS monosaccharides can be manipulated by changes of culture conditions that affect enzyme activities, and that novel fermentation strategies based on this approach may enhance production and biological activity of EPS.
26344261	3	58	theme	lucidum	1004:1010	arg1	monosaccharides					1016:1030	G. lucidum EPS monosaccharides	1001:1030	G. lucidum EPS monosaccharides	1001:1030	In nine tested enzymes, higher enzyme activities were correlated with higher temperature and lower initial pH. Altered mole percentages of galactose and mannose under various culture conditions were associated with activities of α-phosphoglucomutase (PGM) and phosphoglucose isomerase (PGI), respectively, and that of mannose was also associated with phosphomannose isomerase (PMI) activity only under various pH. Our findings suggest that mole percentages of G. lucidum EPS monosaccharides can be manipulated by changes of culture conditions that affect enzyme activities, and that novel fermentation strategies based on this approach may enhance production and biological activity of EPS.
26344261	2	59	theme	monosaccharides	388:402	arg1	percentages					357:367	The mole percentages	348:367	The mole percentages of three major EPS monosaccharides, glucose, galactose and mannose,	348:435	The mole percentages of three major EPS monosaccharides, glucose, galactose and mannose, varied depending on culture conditions and the resulting EPS displayed differing anti-tumor activities.
26344261	1	60	theme	EPS	268:270	arg1	synthesis					272:280	EPS synthesis	268:280	EPS synthesis	268:280	We investigated the relationship between monosaccharide composition of Ganoderma lucidum exopolysaccharide (EPS) and activities of EPS synthesis enzymes under various culture temperatures and initial pH values.
27987982	5	0	theme	biocomposites	704:716	arg1	performance					667:677	the dielectric performance	652:677	the dielectric performance of the corresponding TPS biocomposites	652:716	The hydrophobic/hydrophilic character of the modifiers governed the dielectric performance of the corresponding TPS biocomposites.
27987982	8	1	theme	constant	1051:1058	arg1	field					1060:1064	constant field	1051:1064	constant field	1051:1064	Energy density, prior and after water evaporation, was also determined at constant field.
27987982	9	2	theme	originated	1166:1175	arg1	phenomena					1189:1197	' originated interfacial phenomena	1164:1197	' originated interfacial phenomena	1164:1197	By employing dielectric reinforcing function the contributions of water-assisted and constituents' originated interfacial phenomena could be separated.
27987982	9	3	theme	interfacial	1177:1187	arg1	phenomena					1189:1197	' originated interfacial phenomena	1164:1197	' originated interfacial phenomena	1164:1197	By employing dielectric reinforcing function the contributions of water-assisted and constituents' originated interfacial phenomena could be separated.
27987982	0	4	theme	dielectric	100:109	arg1	study					124:128	A broadband dielectric spectroscopy study	88:128	natural rubber latex: A broadband dielectric spectroscopy study	66:128	Thermoplastic starch modified with microfibrillated cellulose and natural rubber latex: A broadband dielectric spectroscopy study.
27987982	0	5	theme	broadband	90:98	arg1	study					124:128	A broadband dielectric spectroscopy study	88:128	natural rubber latex: A broadband dielectric spectroscopy study	66:128	Thermoplastic starch modified with microfibrillated cellulose and natural rubber latex: A broadband dielectric spectroscopy study.
27987982	5	6	theme	dielectric	656:665	arg1	performance					667:677	the dielectric performance	652:677	the dielectric performance of the corresponding TPS biocomposites	652:716	The hydrophobic/hydrophilic character of the modifiers governed the dielectric performance of the corresponding TPS biocomposites.
27987982	8	7	theme	water	1009:1013	arg1	evaporation					1015:1025	water evaporation	1009:1025	water evaporation	1009:1025	Energy density, prior and after water evaporation, was also determined at constant field.
27987982	6	8	theme	interfacial	813:823	arg1	polarization					825:836	matrix-water-reinforcement interfacial polarization	786:836	matrix-water-reinforcement interfacial polarization	786:836	Conducted analysis revealed two relaxation processes attributed to matrix-water-reinforcement interfacial polarization and glass to rubber transition of the TPS.
27987982	0	9	theme	natural	66:72	arg1	latex					81:85	natural rubber latex	66:85	natural rubber latex: A broadband dielectric spectroscopy study	66:128	Thermoplastic starch modified with microfibrillated cellulose and natural rubber latex: A broadband dielectric spectroscopy study.
27987982	3	10	from	spectroscopy	412:423	arg1	ranges					458:463	the temperature and frequency ranges	428:463	ranges	458:463	The dielectric properties of the TPS composites were examined via broadband dielectric spectroscopy in the temperature and frequency ranges of 30°C-65°C and 0.1Hz-10MHz, respectively.
27987982	9	11	dep	water-assisted	1133:1146	arg1	phenomena					1189:1197	' originated interfacial phenomena	1164:1197	' originated interfacial phenomena	1164:1197	By employing dielectric reinforcing function the contributions of water-assisted and constituents' originated interfacial phenomena could be separated.
27987982	6	12	theme	matrix-water-reinforcement	786:811	arg1	polarization					825:836	matrix-water-reinforcement interfacial polarization	786:836	matrix-water-reinforcement interfacial polarization	786:836	Conducted analysis revealed two relaxation processes attributed to matrix-water-reinforcement interfacial polarization and glass to rubber transition of the TPS.
27987982	0	13	theme	spectroscopy	111:122	arg1	study					124:128	A broadband dielectric spectroscopy study	88:128	natural rubber latex: A broadband dielectric spectroscopy study	66:128	Thermoplastic starch modified with microfibrillated cellulose and natural rubber latex: A broadband dielectric spectroscopy study.
27987982	9	14	theme	reinforcing	1091:1101	arg1	function					1103:1110	dielectric reinforcing function	1080:1110	dielectric reinforcing function	1080:1110	By employing dielectric reinforcing function the contributions of water-assisted and constituents' originated interfacial phenomena could be separated.
27987982	3	15	theme	0.1Hz-10MHz	482:492	arg1	ranges					458:463	the temperature and frequency ranges	428:463	ranges	458:463	The dielectric properties of the TPS composites were examined via broadband dielectric spectroscopy in the temperature and frequency ranges of 30°C-65°C and 0.1Hz-10MHz, respectively.
27987982	5	16	theme	corresponding	686:698	arg1	biocomposites					704:716	the corresponding TPS biocomposites	682:716	the corresponding TPS biocomposites	682:716	The hydrophobic/hydrophilic character of the modifiers governed the dielectric performance of the corresponding TPS biocomposites.
27987982	8	17	theme	Energy	977:982	arg1	density					984:990	Energy density	977:990	Energy density	977:990	Energy density, prior and after water evaporation, was also determined at constant field.
27987982	0	18	theme	Thermoplastic	0:12	arg1	starch					14:19	Thermoplastic starch	0:19	Thermoplastic starch	0:19	Thermoplastic starch modified with microfibrillated cellulose and natural rubber latex: A broadband dielectric spectroscopy study.
27987982	5	19	theme	TPS	700:702	arg1	biocomposites					704:716	the corresponding TPS biocomposites	682:716	the corresponding TPS biocomposites	682:716	The hydrophobic/hydrophilic character of the modifiers governed the dielectric performance of the corresponding TPS biocomposites.
27987982	3	20	theme	30°C-65°C	468:476	arg1	ranges					458:463	the temperature and frequency ranges	428:463	ranges	458:463	The dielectric properties of the TPS composites were examined via broadband dielectric spectroscopy in the temperature and frequency ranges of 30°C-65°C and 0.1Hz-10MHz, respectively.
27987982	3	21	theme	dielectric	401:410	arg1	spectroscopy					412:423	broadband dielectric spectroscopy	391:423	broadband dielectric spectroscopy in the temperature and frequency ranges of 30°C-65°C and 0.1Hz-10MHz, respectively	391:506	The dielectric properties of the TPS composites were examined via broadband dielectric spectroscopy in the temperature and frequency ranges of 30°C-65°C and 0.1Hz-10MHz, respectively.
27987982	4	22	theme	water	581:585	arg1	effect					562:567	the effect	558:567	the effect of absorbed water	558:585	Each specimen was tested twice in order to study the effect of absorbed water.
27987982	5	23	theme	modifiers	633:641	arg1	character					616:624	The hydrophobic/hydrophilic character	588:624	The hydrophobic/hydrophilic character of the modifiers	588:641	The hydrophobic/hydrophilic character of the modifiers governed the dielectric performance of the corresponding TPS biocomposites.
27987982	0	24	dep	latex	81:85	arg1	study					124:128	A broadband dielectric spectroscopy study	88:128	natural rubber latex: A broadband dielectric spectroscopy study	66:128	Thermoplastic starch modified with microfibrillated cellulose and natural rubber latex: A broadband dielectric spectroscopy study.
27987982	6	25	theme	relaxation	751:760	arg1	processes					762:770	two relaxation processes	747:770	two relaxation processes attributed to matrix-water-reinforcement interfacial polarization and glass to rubber transition of the TPS	747:878	Conducted analysis revealed two relaxation processes attributed to matrix-water-reinforcement interfacial polarization and glass to rubber transition of the TPS.
27987982	1	26	theme	natural	215:221	arg1	rubber					223:228	natural rubber	215:228	natural rubber	215:228	Thermoplastic starch (TPS) biocomposites modified with cellulose microfibers and/or natural rubber were prepared via extrusion compounding.
27987982	9	27	theme	dielectric	1080:1089	arg1	function					1103:1110	dielectric reinforcing function	1080:1110	dielectric reinforcing function	1080:1110	By employing dielectric reinforcing function the contributions of water-assisted and constituents' originated interfacial phenomena could be separated.
27987982	7	28	theme	water	896:900	arg1	Evaporation					881:891	Evaporation	881:891	Evaporation of water	881:900	Evaporation of water significantly affected the first process and only slightly the second one.
27987982	7	29	theme	first	929:933	arg1	process					935:941	the first process	925:941	the first process	925:941	Evaporation of water significantly affected the first process and only slightly the second one.
27987982	3	30	theme	frequency	448:456	arg1	ranges					458:463	the temperature and frequency ranges	428:463	ranges	458:463	The dielectric properties of the TPS composites were examined via broadband dielectric spectroscopy in the temperature and frequency ranges of 30°C-65°C and 0.1Hz-10MHz, respectively.
27987982	9	31	theme	water-assisted	1133:1146	arg1	contributions					1116:1128	the contributions	1112:1128	the contributions of water-assisted and constituents' originated interfacial phenomena	1112:1197	By employing dielectric reinforcing function the contributions of water-assisted and constituents' originated interfacial phenomena could be separated.
27987982	9	32	theme	constituents	1152:1163	arg1	contributions					1116:1128	the contributions	1112:1128	the contributions of water-assisted and constituents' originated interfacial phenomena	1112:1197	By employing dielectric reinforcing function the contributions of water-assisted and constituents' originated interfacial phenomena could be separated.
27987982	0	33	theme	microfibrillated	35:50	arg1	cellulose					52:60	microfibrillated cellulose	35:60	microfibrillated cellulose	35:60	Thermoplastic starch modified with microfibrillated cellulose and natural rubber latex: A broadband dielectric spectroscopy study.
27987982	7	34	theme	second	965:970	arg1	one					972:974	the second one	961:974	the second one	961:974	Evaporation of water significantly affected the first process and only slightly the second one.
27987982	3	35	theme	temperature	432:442	arg1	ranges					458:463	the temperature and frequency ranges	428:463	ranges	458:463	The dielectric properties of the TPS composites were examined via broadband dielectric spectroscopy in the temperature and frequency ranges of 30°C-65°C and 0.1Hz-10MHz, respectively.
27987982	3	36	theme	dielectric	329:338	arg1	properties					340:349	The dielectric properties	325:349	The dielectric properties of the TPS composites	325:371	The dielectric properties of the TPS composites were examined via broadband dielectric spectroscopy in the temperature and frequency ranges of 30°C-65°C and 0.1Hz-10MHz, respectively.
27987982	0	37	theme	rubber	74:79	arg1	latex					81:85	natural rubber latex	66:85	natural rubber latex: A broadband dielectric spectroscopy study	66:128	Thermoplastic starch modified with microfibrillated cellulose and natural rubber latex: A broadband dielectric spectroscopy study.
27987982	4	38	theme	absorbed	572:579	arg1	water					581:585	absorbed water	572:585	absorbed water	572:585	Each specimen was tested twice in order to study the effect of absorbed water.
27987982	1	39	theme	Thermoplastic	131:143	arg1	biocomposites					158:170	Thermoplastic starch (TPS) biocomposites	131:170	Thermoplastic starch (TPS) biocomposites modified with cellulose microfibers and/or natural rubber	131:228	Thermoplastic starch (TPS) biocomposites modified with cellulose microfibers and/or natural rubber were prepared via extrusion compounding.
27987982	6	40	theme	TPS	876:878	arg1	transition					858:867	glass to rubber transition	842:867	glass to rubber transition	842:867	Conducted analysis revealed two relaxation processes attributed to matrix-water-reinforcement interfacial polarization and glass to rubber transition of the TPS.
27987982	6	40	theme	TPS	876:878	arg1	polarization					825:836	matrix-water-reinforcement interfacial polarization	786:836	matrix-water-reinforcement interfacial polarization	786:836	Conducted analysis revealed two relaxation processes attributed to matrix-water-reinforcement interfacial polarization and glass to rubber transition of the TPS.
27987982	5	41	theme	hydrophobic/hydrophilic	592:614	arg1	character					616:624	The hydrophobic/hydrophilic character	588:624	The hydrophobic/hydrophilic character of the modifiers	588:641	The hydrophobic/hydrophilic character of the modifiers governed the dielectric performance of the corresponding TPS biocomposites.
27987982	1	42	theme	starch	145:150	arg1	biocomposites					158:170	Thermoplastic starch (TPS) biocomposites	131:170	Thermoplastic starch (TPS) biocomposites modified with cellulose microfibers and/or natural rubber	131:228	Thermoplastic starch (TPS) biocomposites modified with cellulose microfibers and/or natural rubber were prepared via extrusion compounding.
27987982	6	43	theme	Conducted	719:727	arg1	analysis					729:736	Conducted analysis	719:736	Conducted analysis	719:736	Conducted analysis revealed two relaxation processes attributed to matrix-water-reinforcement interfacial polarization and glass to rubber transition of the TPS.
27987982	6	44	theme	rubber	851:856	arg1	transition					858:867	glass to rubber transition	842:867	glass to rubber transition	842:867	Conducted analysis revealed two relaxation processes attributed to matrix-water-reinforcement interfacial polarization and glass to rubber transition of the TPS.
27987982	1	45	theme	extrusion	248:256	arg1	compounding					258:268	extrusion compounding	248:268	extrusion compounding	248:268	Thermoplastic starch (TPS) biocomposites modified with cellulose microfibers and/or natural rubber were prepared via extrusion compounding.
27987982	3	46	theme	TPS	358:360	arg1	composites					362:371	the TPS composites	354:371	the TPS composites	354:371	The dielectric properties of the TPS composites were examined via broadband dielectric spectroscopy in the temperature and frequency ranges of 30°C-65°C and 0.1Hz-10MHz, respectively.
27987982	6	47	theme	glass	842:846	arg1	transition					858:867	glass to rubber transition	842:867	glass to rubber transition	842:867	Conducted analysis revealed two relaxation processes attributed to matrix-water-reinforcement interfacial polarization and glass to rubber transition of the TPS.
27987982	1	48	theme	TPS	153:155	arg1	biocomposites					158:170	Thermoplastic starch (TPS) biocomposites	131:170	Thermoplastic starch (TPS) biocomposites modified with cellulose microfibers and/or natural rubber	131:228	Thermoplastic starch (TPS) biocomposites modified with cellulose microfibers and/or natural rubber were prepared via extrusion compounding.
27987982	1	49	theme	cellulose	186:194	arg1	microfibers					196:206	cellulose microfibers	186:206	cellulose microfibers	186:206	Thermoplastic starch (TPS) biocomposites modified with cellulose microfibers and/or natural rubber were prepared via extrusion compounding.
27987982	3	50	theme	composites	362:371	arg1	properties					340:349	The dielectric properties	325:349	The dielectric properties of the TPS composites	325:371	The dielectric properties of the TPS composites were examined via broadband dielectric spectroscopy in the temperature and frequency ranges of 30°C-65°C and 0.1Hz-10MHz, respectively.
27987982	6	51	dep	transition	858:867	arg1	to					848:849	to	848:849	to	848:849	Conducted analysis revealed two relaxation processes attributed to matrix-water-reinforcement interfacial polarization and glass to rubber transition of the TPS.
27987982	3	52	theme	broadband	391:399	arg1	spectroscopy					412:423	broadband dielectric spectroscopy	391:423	broadband dielectric spectroscopy in the temperature and frequency ranges of 30°C-65°C and 0.1Hz-10MHz, respectively	391:506	The dielectric properties of the TPS composites were examined via broadband dielectric spectroscopy in the temperature and frequency ranges of 30°C-65°C and 0.1Hz-10MHz, respectively.
28946308	0	0	theme	fruiting	83:90	arg1	bodies					92:97	the fruiting bodies	79:97	the fruiting bodies of Amillariella mellea	79:120	Structure elucidation and immunomodulatory activity of a β-glucan derived from the fruiting bodies of Amillariella mellea.
28946308	6	1	theme	NF-κB/MAPK	866:875	arg1	pathways					887:894	NF-κB/MAPK signaling pathways	866:894	NF-κB/MAPK signaling pathways	866:894	Our data demonstrate that AAMP-A70 activates macrophages via NF-κB/MAPK signaling pathways and the TLR2 receptor.
28946308	2	2	theme	main	334:337	arg1	chain					339:343	a main chain	332:343	a main chain that consists of β-d-(1→6) linked Glcρ residues substituted at O-3 by β-Glcρ or α-d-(1→6)-linked Galρ side chains	332:457	Compositional analysis and 1H and 13C NMR spectra indicate that AAMP-A70 is a branched β-glucan with a main chain that consists of β-d-(1→6) linked Glcρ residues substituted at O-3 by β-Glcρ or α-d-(1→6)-linked Galρ side chains.
28946308	4	3	theme	MAPKs	692:696	arg1	phosphorylation					673:687	phosphorylation	673:687	phosphorylation of MAPKs	673:696	Mechanistically, AAMP-A70 promotes degradation of IκB-α and nuclear translocation of the NF-κB p65 subunit, and enhances phosphorylation of MAPKs.
28946308	2	4	theme	branched	309:316	arg1	AAMP-A70					295:302	AAMP-A70	295:302	AAMP-A70	295:302	Compositional analysis and 1H and 13C NMR spectra indicate that AAMP-A70 is a branched β-glucan with a main chain that consists of β-d-(1→6) linked Glcρ residues substituted at O-3 by β-Glcρ or α-d-(1→6)-linked Galρ side chains.
28946308	2	4	theme	branched	309:316	arg1	β-glucan					318:325	a branched β-glucan	307:325	a branched β-glucan with a main chain that consists of β-d-(1→6) linked Glcρ residues substituted at O-3 by β-Glcρ or α-d-(1→6)-linked Galρ side chains	307:457	Compositional analysis and 1H and 13C NMR spectra indicate that AAMP-A70 is a branched β-glucan with a main chain that consists of β-d-(1→6) linked Glcρ residues substituted at O-3 by β-Glcρ or α-d-(1→6)-linked Galρ side chains.
28946308	0	5	theme	mellea	115:120	arg1	bodies					92:97	the fruiting bodies	79:97	the fruiting bodies of Amillariella mellea	79:120	Structure elucidation and immunomodulatory activity of a β-glucan derived from the fruiting bodies of Amillariella mellea.
28946308	2	6	theme	Glcρ	379:382	arg1	residues					384:391	β-d-(1→6) linked Glcρ residues	362:391	β-d-(1→6) linked Glcρ residues substituted at O-3 by β-Glcρ or α-d-(1→6)-linked Galρ side chains	362:457	Compositional analysis and 1H and 13C NMR spectra indicate that AAMP-A70 is a branched β-glucan with a main chain that consists of β-d-(1→6) linked Glcρ residues substituted at O-3 by β-Glcρ or α-d-(1→6)-linked Galρ side chains.
28946308	2	7	theme	NMR	269:271	arg1	spectra					273:279	NMR spectra	269:279	NMR spectra	269:279	Compositional analysis and 1H and 13C NMR spectra indicate that AAMP-A70 is a branched β-glucan with a main chain that consists of β-d-(1→6) linked Glcρ residues substituted at O-3 by β-Glcρ or α-d-(1→6)-linked Galρ side chains.
28946308	0	8	theme	Amillariella	102:113	arg1	mellea					115:120	Amillariella mellea	102:120	Amillariella mellea	102:120	Structure elucidation and immunomodulatory activity of a β-glucan derived from the fruiting bodies of Amillariella mellea.
28946308	3	9	theme	macrophage	479:488	arg1	phagocytosis					490:501	macrophage phagocytosis	479:501	macrophage phagocytosis	479:501	AAMP-A70 increases macrophage phagocytosis and secretion of NO, ROS, TNF-α, IL-6 and IL-1β.
28946308	3	10	theme	NO	520:521	arg1	phagocytosis					490:501	macrophage phagocytosis	479:501	macrophage phagocytosis	479:501	AAMP-A70 increases macrophage phagocytosis and secretion of NO, ROS, TNF-α, IL-6 and IL-1β.
28946308	3	10	theme	NO	520:521	arg1	secretion					507:515	secretion	507:515	secretion	507:515	AAMP-A70 increases macrophage phagocytosis and secretion of NO, ROS, TNF-α, IL-6 and IL-1β.
28946308	0	11	theme	Structure	0:8	arg1	elucidation					10:20	Structure elucidation	0:20	Structure elucidation	0:20	Structure elucidation and immunomodulatory activity of a β-glucan derived from the fruiting bodies of Amillariella mellea.
28946308	4	12	theme	subunit	651:657	arg1	translocation					620:632	nuclear translocation	612:632	nuclear translocation of the NF-κB p65 subunit	612:657	Mechanistically, AAMP-A70 promotes degradation of IκB-α and nuclear translocation of the NF-κB p65 subunit, and enhances phosphorylation of MAPKs.
28946308	4	12	theme	subunit	651:657	arg1	degradation					587:597	degradation	587:597	degradation of IκB-α	587:606	Mechanistically, AAMP-A70 promotes degradation of IκB-α and nuclear translocation of the NF-κB p65 subunit, and enhances phosphorylation of MAPKs.
28946308	2	13	dep	1H	258:259	arg1	spectra					273:279	NMR spectra	269:279	NMR spectra	269:279	Compositional analysis and 1H and 13C NMR spectra indicate that AAMP-A70 is a branched β-glucan with a main chain that consists of β-d-(1→6) linked Glcρ residues substituted at O-3 by β-Glcρ or α-d-(1→6)-linked Galρ side chains.
28946308	0	14	theme	immunomodulatory	26:41	arg1	activity					43:50	immunomodulatory activity	26:50	immunomodulatory activity	26:50	Structure elucidation and immunomodulatory activity of a β-glucan derived from the fruiting bodies of Amillariella mellea.
28946308	5	15	theme	function	718:725	arg1	antibody					736:743	the function blocking antibody	714:743	the function blocking antibody to TLR2	714:751	In particular, the function blocking antibody to TLR2 substantially suppresses TNF-α and IL-6 production.
28946308	3	16	theme	TNF-α	529:533	arg1	phagocytosis					490:501	macrophage phagocytosis	479:501	macrophage phagocytosis	479:501	AAMP-A70 increases macrophage phagocytosis and secretion of NO, ROS, TNF-α, IL-6 and IL-1β.
28946308	3	16	theme	TNF-α	529:533	arg1	secretion					507:515	secretion	507:515	secretion	507:515	AAMP-A70 increases macrophage phagocytosis and secretion of NO, ROS, TNF-α, IL-6 and IL-1β.
28946308	2	17	with	β-glucan	318:325	arg1	chain					339:343	a main chain	332:343	a main chain that consists of β-d-(1→6) linked Glcρ residues substituted at O-3 by β-Glcρ or α-d-(1→6)-linked Galρ side chains	332:457	Compositional analysis and 1H and 13C NMR spectra indicate that AAMP-A70 is a branched β-glucan with a main chain that consists of β-d-(1→6) linked Glcρ residues substituted at O-3 by β-Glcρ or α-d-(1→6)-linked Galρ side chains.
28946308	1	18	theme	fruiting	191:198	arg1	bodies					200:205	the fruiting bodies	187:205	the fruiting bodies of Amillariella mellea	187:228	A novel polysaccharide AAMP-A70 (5.6kDa) has been purified from the fruiting bodies of Amillariella mellea.
28946308	2	19	theme	Compositional	231:243	arg1	analysis					245:252	Compositional analysis	231:252	Compositional analysis	231:252	Compositional analysis and 1H and 13C NMR spectra indicate that AAMP-A70 is a branched β-glucan with a main chain that consists of β-d-(1→6) linked Glcρ residues substituted at O-3 by β-Glcρ or α-d-(1→6)-linked Galρ side chains.
28946308	4	20	theme	IκB-α	602:606	arg1	translocation					620:632	nuclear translocation	612:632	nuclear translocation of the NF-κB p65 subunit	612:657	Mechanistically, AAMP-A70 promotes degradation of IκB-α and nuclear translocation of the NF-κB p65 subunit, and enhances phosphorylation of MAPKs.
28946308	4	20	theme	IκB-α	602:606	arg1	degradation					587:597	degradation	587:597	degradation of IκB-α	587:606	Mechanistically, AAMP-A70 promotes degradation of IκB-α and nuclear translocation of the NF-κB p65 subunit, and enhances phosphorylation of MAPKs.
28946308	2	21	theme	linked	372:377	arg1	residues					384:391	β-d-(1→6) linked Glcρ residues	362:391	β-d-(1→6) linked Glcρ residues substituted at O-3 by β-Glcρ or α-d-(1→6)-linked Galρ side chains	362:457	Compositional analysis and 1H and 13C NMR spectra indicate that AAMP-A70 is a branched β-glucan with a main chain that consists of β-d-(1→6) linked Glcρ residues substituted at O-3 by β-Glcρ or α-d-(1→6)-linked Galρ side chains.
28946308	6	22	theme	TLR2	904:907	arg1	receptor					909:916	the TLR2 receptor	900:916	the TLR2 receptor	900:916	Our data demonstrate that AAMP-A70 activates macrophages via NF-κB/MAPK signaling pathways and the TLR2 receptor.
28946308	7	23	theme	food	957:960	arg1	AAMP-A70					928:935	AAMP-A70	928:935	AAMP-A70	928:935	Overall, AAMP-A70 may serve as a good food supplement to enhance immunity.
28946308	7	23	theme	food	957:960	arg1	supplement					962:971	a good food supplement	950:971	a good food supplement to enhance immunity	950:991	Overall, AAMP-A70 may serve as a good food supplement to enhance immunity.
28946308	2	24	theme	side	447:450	arg1	chains					452:457	α-d-(1→6)-linked Galρ side chains	425:457	α-d-(1→6)-linked Galρ side chains	425:457	Compositional analysis and 1H and 13C NMR spectra indicate that AAMP-A70 is a branched β-glucan with a main chain that consists of β-d-(1→6) linked Glcρ residues substituted at O-3 by β-Glcρ or α-d-(1→6)-linked Galρ side chains.
28946308	0	25	theme	β-glucan	57:64	arg1	elucidation					10:20	Structure elucidation	0:20	Structure elucidation	0:20	Structure elucidation and immunomodulatory activity of a β-glucan derived from the fruiting bodies of Amillariella mellea.
28946308	0	25	theme	β-glucan	57:64	arg1	activity					43:50	immunomodulatory activity	26:50	immunomodulatory activity	26:50	Structure elucidation and immunomodulatory activity of a β-glucan derived from the fruiting bodies of Amillariella mellea.
28946308	3	26	theme	ROS	524:526	arg1	phagocytosis					490:501	macrophage phagocytosis	479:501	macrophage phagocytosis	479:501	AAMP-A70 increases macrophage phagocytosis and secretion of NO, ROS, TNF-α, IL-6 and IL-1β.
28946308	3	26	theme	ROS	524:526	arg1	secretion					507:515	secretion	507:515	secretion	507:515	AAMP-A70 increases macrophage phagocytosis and secretion of NO, ROS, TNF-α, IL-6 and IL-1β.
28946308	4	27	theme	p65	647:649	arg1	subunit					651:657	the NF-κB p65 subunit	637:657	the NF-κB p65 subunit	637:657	Mechanistically, AAMP-A70 promotes degradation of IκB-α and nuclear translocation of the NF-κB p65 subunit, and enhances phosphorylation of MAPKs.
28946308	5	28	theme	blocking	727:734	arg1	antibody					736:743	the function blocking antibody	714:743	the function blocking antibody to TLR2	714:751	In particular, the function blocking antibody to TLR2 substantially suppresses TNF-α and IL-6 production.
28946308	2	29	link	linked	372:377	arg1	residues					384:391	β-d-(1→6) linked Glcρ residues	362:391	β-d-(1→6) linked Glcρ residues substituted at O-3 by β-Glcρ or α-d-(1→6)-linked Galρ side chains	362:457	Compositional analysis and 1H and 13C NMR spectra indicate that AAMP-A70 is a branched β-glucan with a main chain that consists of β-d-(1→6) linked Glcρ residues substituted at O-3 by β-Glcρ or α-d-(1→6)-linked Galρ side chains.
28946308	4	30	theme	NF-κB	641:645	arg1	subunit					651:657	the NF-κB p65 subunit	637:657	the NF-κB p65 subunit	637:657	Mechanistically, AAMP-A70 promotes degradation of IκB-α and nuclear translocation of the NF-κB p65 subunit, and enhances phosphorylation of MAPKs.
28946308	3	31	theme	IL-1β	545:549	arg1	phagocytosis					490:501	macrophage phagocytosis	479:501	macrophage phagocytosis	479:501	AAMP-A70 increases macrophage phagocytosis and secretion of NO, ROS, TNF-α, IL-6 and IL-1β.
28946308	3	31	theme	IL-1β	545:549	arg1	secretion					507:515	secretion	507:515	secretion	507:515	AAMP-A70 increases macrophage phagocytosis and secretion of NO, ROS, TNF-α, IL-6 and IL-1β.
28946308	2	32	link	-linked	434:440	arg1	chains					452:457	α-d-(1→6)-linked Galρ side chains	425:457	α-d-(1→6)-linked Galρ side chains	425:457	Compositional analysis and 1H and 13C NMR spectra indicate that AAMP-A70 is a branched β-glucan with a main chain that consists of β-d-(1→6) linked Glcρ residues substituted at O-3 by β-Glcρ or α-d-(1→6)-linked Galρ side chains.
28946308	1	33	theme	novel	125:129	arg1	5.6kDa					156:161	5.6kDa	156:161	5.6kDa	156:161	A novel polysaccharide AAMP-A70 (5.6kDa) has been purified from the fruiting bodies of Amillariella mellea.
28946308	1	33	theme	novel	125:129	arg1	AAMP-A70					146:153	A novel polysaccharide AAMP-A70	123:153	A novel polysaccharide AAMP-A70 (5.6kDa)	123:162	A novel polysaccharide AAMP-A70 (5.6kDa) has been purified from the fruiting bodies of Amillariella mellea.
28946308	1	34	theme	Amillariella	210:221	arg1	mellea					223:228	Amillariella mellea	210:228	Amillariella mellea	210:228	A novel polysaccharide AAMP-A70 (5.6kDa) has been purified from the fruiting bodies of Amillariella mellea.
28946308	7	35	theme	good	952:955	arg1	AAMP-A70					928:935	AAMP-A70	928:935	AAMP-A70	928:935	Overall, AAMP-A70 may serve as a good food supplement to enhance immunity.
28946308	7	35	theme	good	952:955	arg1	supplement					962:971	a good food supplement	950:971	a good food supplement to enhance immunity	950:991	Overall, AAMP-A70 may serve as a good food supplement to enhance immunity.
28946308	1	36	theme	polysaccharide	131:144	arg1	5.6kDa					156:161	5.6kDa	156:161	5.6kDa	156:161	A novel polysaccharide AAMP-A70 (5.6kDa) has been purified from the fruiting bodies of Amillariella mellea.
28946308	1	36	theme	polysaccharide	131:144	arg1	AAMP-A70					146:153	A novel polysaccharide AAMP-A70	123:153	A novel polysaccharide AAMP-A70 (5.6kDa)	123:162	A novel polysaccharide AAMP-A70 (5.6kDa) has been purified from the fruiting bodies of Amillariella mellea.
28946308	1	37	theme	mellea	223:228	arg1	bodies					200:205	the fruiting bodies	187:205	the fruiting bodies of Amillariella mellea	187:228	A novel polysaccharide AAMP-A70 (5.6kDa) has been purified from the fruiting bodies of Amillariella mellea.
28946308	2	38	theme	Galρ	442:445	arg1	chains					452:457	α-d-(1→6)-linked Galρ side chains	425:457	α-d-(1→6)-linked Galρ side chains	425:457	Compositional analysis and 1H and 13C NMR spectra indicate that AAMP-A70 is a branched β-glucan with a main chain that consists of β-d-(1→6) linked Glcρ residues substituted at O-3 by β-Glcρ or α-d-(1→6)-linked Galρ side chains.
28946308	5	39	theme	IL-6	788:791	arg1	production					793:802	IL-6 production	788:802	IL-6 production	788:802	In particular, the function blocking antibody to TLR2 substantially suppresses TNF-α and IL-6 production.
28946308	3	40	theme	IL-6	536:539	arg1	phagocytosis					490:501	macrophage phagocytosis	479:501	macrophage phagocytosis	479:501	AAMP-A70 increases macrophage phagocytosis and secretion of NO, ROS, TNF-α, IL-6 and IL-1β.
28946308	3	40	theme	IL-6	536:539	arg1	secretion					507:515	secretion	507:515	secretion	507:515	AAMP-A70 increases macrophage phagocytosis and secretion of NO, ROS, TNF-α, IL-6 and IL-1β.
28946308	2	41	theme	-linked	434:440	arg1	chains					452:457	α-d-(1→6)-linked Galρ side chains	425:457	α-d-(1→6)-linked Galρ side chains	425:457	Compositional analysis and 1H and 13C NMR spectra indicate that AAMP-A70 is a branched β-glucan with a main chain that consists of β-d-(1→6) linked Glcρ residues substituted at O-3 by β-Glcρ or α-d-(1→6)-linked Galρ side chains.
28946308	6	42	theme	signaling	877:885	arg1	pathways					887:894	NF-κB/MAPK signaling pathways	866:894	NF-κB/MAPK signaling pathways	866:894	Our data demonstrate that AAMP-A70 activates macrophages via NF-κB/MAPK signaling pathways and the TLR2 receptor.
28946308	4	43	theme	nuclear	612:618	arg1	translocation					620:632	nuclear translocation	612:632	nuclear translocation of the NF-κB p65 subunit	612:657	Mechanistically, AAMP-A70 promotes degradation of IκB-α and nuclear translocation of the NF-κB p65 subunit, and enhances phosphorylation of MAPKs.
24702917	6	0	theme	EPS	985:987	arg1	fractions					989:997	EPS fractions	985:997	EPS fractions	985:997	Both of EPS fractions could significantly stimulate splenocyte proliferation in vitro, indicating two EPS fractions had the potential immunomodulatory activity.
24702917	5	1	dep	10	876:877	arg1	6					879:879	6	879:879	6	879:879	Whilst Fraction S2, with a high average molecular weight of 1.23 × 10(6)Da, contained rhamnose, glucose and galactose in a molar ratio of approximately 1.73:1.47:1.00.
24702917	1	2	attach	isolated	156:163	arg2	KF5					136:138	Lactobacillus rhamnosus KF5	112:138	Lactobacillus rhamnosus KF5	112:138	Lactobacillus rhamnosus KF5, a strain newly isolated from the faeces of a healthy human volunteer, has been shown to produce the exopolysaccharides (EPS) in skim milk.
24702917	1	2	attach	isolated	156:163	arg1	faeces					174:179	the faeces	170:179	the faeces of a healthy human volunteer	170:208	Lactobacillus rhamnosus KF5, a strain newly isolated from the faeces of a healthy human volunteer, has been shown to produce the exopolysaccharides (EPS) in skim milk.
24702917	1	2	attach	isolated	156:163	arg2	strain					143:148	a strain	141:148	a strain newly isolated from the faeces of a healthy human volunteer	141:208	Lactobacillus rhamnosus KF5, a strain newly isolated from the faeces of a healthy human volunteer, has been shown to produce the exopolysaccharides (EPS) in skim milk.
24702917	4	3	theme	EPS	804:806	arg1	S1					756:757	S1	756:757	S1	756:757	Such monosaccharide composition of the exopolysaccharide by lactic acid bacteria has not been reported so far, and S1 is likely to be an unusual type of microbial EPS.
24702917	4	3	theme	EPS	804:806	arg1	type					786:789	an unusual type	775:789	an unusual type of microbial EPS	775:806	Such monosaccharide composition of the exopolysaccharide by lactic acid bacteria has not been reported so far, and S1 is likely to be an unusual type of microbial EPS.
24702917	6	4	theme	splenocyte	1029:1038	arg1	proliferation					1040:1052	splenocyte proliferation	1029:1052	splenocyte proliferation	1029:1052	Both of EPS fractions could significantly stimulate splenocyte proliferation in vitro, indicating two EPS fractions had the potential immunomodulatory activity.
24702917	5	5	theme	Da	881:882	arg1	weight					859:864	a high average molecular weight	834:864	a high average molecular weight of 1.23 × 10(6)Da	834:882	Whilst Fraction S2, with a high average molecular weight of 1.23 × 10(6)Da, contained rhamnose, glucose and galactose in a molar ratio of approximately 1.73:1.47:1.00.
24702917	3	6	theme	Da	501:502	arg1	weight					479:484	a low average molecular weight	455:484	a low average molecular weight of 1.36 × 10(4)Da	455:502	Fraction S1, with a low average molecular weight of 1.36 × 10(4)Da, was composed of glucose, arabinose, glucosamine, galactosamine and galactose in an approximate molar ratio of 2.03:1.29:1.25:0.72:0.61.
24702917	2	7	theme	EPS	284:286	arg1	fractions					288:296	Two EPS fractions	280:296	Two EPS fractions	280:296	Two EPS fractions were separated from the fermented skim milk by removing proteins, ethanol precipitation, anion-exchange and gel permeation chromatography.
24702917	4	8	theme	exopolysaccharide	680:696	arg1	composition					661:671	Such monosaccharide composition	641:671	Such monosaccharide composition of the exopolysaccharide by lactic acid bacteria	641:720	Such monosaccharide composition of the exopolysaccharide by lactic acid bacteria has not been reported so far, and S1 is likely to be an unusual type of microbial EPS.
24702917	3	9	theme	low	457:459	arg1	weight					479:484	a low average molecular weight	455:484	a low average molecular weight of 1.36 × 10(4)Da	455:502	Fraction S1, with a low average molecular weight of 1.36 × 10(4)Da, was composed of glucose, arabinose, glucosamine, galactosamine and galactose in an approximate molar ratio of 2.03:1.29:1.25:0.72:0.61.
24702917	6	10	theme	EPS	1079:1081	arg1	fractions					1083:1091	two EPS fractions	1075:1091	two EPS fractions	1075:1091	Both of EPS fractions could significantly stimulate splenocyte proliferation in vitro, indicating two EPS fractions had the potential immunomodulatory activity.
24702917	3	11	from	galactosamine	554:566	arg1	ratio					606:610	an approximate molar ratio	585:610	an approximate molar ratio of 2.03:1.29:1.25:0.72:0.61	585:638	Fraction S1, with a low average molecular weight of 1.36 × 10(4)Da, was composed of glucose, arabinose, glucosamine, galactosamine and galactose in an approximate molar ratio of 2.03:1.29:1.25:0.72:0.61.
24702917	3	12	theme	Fraction	437:444	arg1	S1					446:447	Fraction S1	437:447	Fraction S1	437:447	Fraction S1, with a low average molecular weight of 1.36 × 10(4)Da, was composed of glucose, arabinose, glucosamine, galactosamine and galactose in an approximate molar ratio of 2.03:1.29:1.25:0.72:0.61.
24702917	4	13	theme	unusual	778:784	arg1	S1					756:757	S1	756:757	S1	756:757	Such monosaccharide composition of the exopolysaccharide by lactic acid bacteria has not been reported so far, and S1 is likely to be an unusual type of microbial EPS.
24702917	4	13	theme	unusual	778:784	arg1	type					786:789	an unusual type	775:789	an unusual type of microbial EPS	775:806	Such monosaccharide composition of the exopolysaccharide by lactic acid bacteria has not been reported so far, and S1 is likely to be an unusual type of microbial EPS.
24702917	0	14	theme	Partial	0:6	arg1	characterization					8:23	Partial characterization	0:23	Partial characterization	0:23	Partial characterization and immunostimulatory activity of exopolysaccharides from Lactobacillus rhamnosus KF5.
24702917	1	15	theme	healthy	186:192	arg1	volunteer					200:208	a healthy human volunteer	184:208	a healthy human volunteer	184:208	Lactobacillus rhamnosus KF5, a strain newly isolated from the faeces of a healthy human volunteer, has been shown to produce the exopolysaccharides (EPS) in skim milk.
24702917	0	16	from	activity	47:54	arg1	Lactobacillus					83:95	Lactobacillus	83:95	Lactobacillus	83:95	Partial characterization and immunostimulatory activity of exopolysaccharides from Lactobacillus rhamnosus KF5.
24702917	4	17	theme	microbial	794:802	arg1	EPS					804:806	microbial EPS	794:806	microbial EPS	794:806	Such monosaccharide composition of the exopolysaccharide by lactic acid bacteria has not been reported so far, and S1 is likely to be an unusual type of microbial EPS.
24702917	1	18	theme	Lactobacillus	112:124	arg1	KF5					136:138	Lactobacillus rhamnosus KF5	112:138	Lactobacillus rhamnosus KF5	112:138	Lactobacillus rhamnosus KF5, a strain newly isolated from the faeces of a healthy human volunteer, has been shown to produce the exopolysaccharides (EPS) in skim milk.
24702917	1	18	theme	Lactobacillus	112:124	arg1	strain					143:148	a strain	141:148	a strain newly isolated from the faeces of a healthy human volunteer	141:208	Lactobacillus rhamnosus KF5, a strain newly isolated from the faeces of a healthy human volunteer, has been shown to produce the exopolysaccharides (EPS) in skim milk.
24702917	3	19	from	galactose	572:580	arg1	ratio					606:610	an approximate molar ratio	585:610	an approximate molar ratio of 2.03:1.29:1.25:0.72:0.61	585:638	Fraction S1, with a low average molecular weight of 1.36 × 10(4)Da, was composed of glucose, arabinose, glucosamine, galactosamine and galactose in an approximate molar ratio of 2.03:1.29:1.25:0.72:0.61.
24702917	2	20	theme	ethanol	364:370	arg1	precipitation					372:384	ethanol precipitation	364:384	ethanol precipitation	364:384	Two EPS fractions were separated from the fermented skim milk by removing proteins, ethanol precipitation, anion-exchange and gel permeation chromatography.
24702917	2	20	theme	ethanol	364:370	arg1	proteins					354:361	proteins	354:361	proteins	354:361	Two EPS fractions were separated from the fermented skim milk by removing proteins, ethanol precipitation, anion-exchange and gel permeation chromatography.
24702917	0	21	from	characterization	8:23	arg1	Lactobacillus					83:95	Lactobacillus	83:95	Lactobacillus	83:95	Partial characterization and immunostimulatory activity of exopolysaccharides from Lactobacillus rhamnosus KF5.
24702917	1	22	theme	skim	269:272	arg1	milk					274:277	skim milk	269:277	skim milk	269:277	Lactobacillus rhamnosus KF5, a strain newly isolated from the faeces of a healthy human volunteer, has been shown to produce the exopolysaccharides (EPS) in skim milk.
24702917	1	23	theme	rhamnosus	126:134	arg1	KF5					136:138	Lactobacillus rhamnosus KF5	112:138	Lactobacillus rhamnosus KF5	112:138	Lactobacillus rhamnosus KF5, a strain newly isolated from the faeces of a healthy human volunteer, has been shown to produce the exopolysaccharides (EPS) in skim milk.
24702917	1	23	theme	rhamnosus	126:134	arg1	strain					143:148	a strain	141:148	a strain newly isolated from the faeces of a healthy human volunteer	141:208	Lactobacillus rhamnosus KF5, a strain newly isolated from the faeces of a healthy human volunteer, has been shown to produce the exopolysaccharides (EPS) in skim milk.
24702917	5	24	from	galactose	917:925	arg1	ratio					938:942	a molar ratio	930:942	a molar ratio of approximately 1.73:1.47:1.00	930:974	Whilst Fraction S2, with a high average molecular weight of 1.23 × 10(6)Da, contained rhamnose, glucose and galactose in a molar ratio of approximately 1.73:1.47:1.00.
24702917	1	25	theme	volunteer	200:208	arg1	faeces					174:179	the faeces	170:179	the faeces of a healthy human volunteer	170:208	Lactobacillus rhamnosus KF5, a strain newly isolated from the faeces of a healthy human volunteer, has been shown to produce the exopolysaccharides (EPS) in skim milk.
24702917	0	26	theme	immunostimulatory	29:45	arg1	activity					47:54	immunostimulatory activity	29:54	immunostimulatory activity	29:54	Partial characterization and immunostimulatory activity of exopolysaccharides from Lactobacillus rhamnosus KF5.
24702917	3	27	dep	10	496:497	arg1	4					499:499	4	499:499	4	499:499	Fraction S1, with a low average molecular weight of 1.36 × 10(4)Da, was composed of glucose, arabinose, glucosamine, galactosamine and galactose in an approximate molar ratio of 2.03:1.29:1.25:0.72:0.61.
24702917	3	28	from	arabinose	530:538	arg1	ratio					606:610	an approximate molar ratio	585:610	an approximate molar ratio of 2.03:1.29:1.25:0.72:0.61	585:638	Fraction S1, with a low average molecular weight of 1.36 × 10(4)Da, was composed of glucose, arabinose, glucosamine, galactosamine and galactose in an approximate molar ratio of 2.03:1.29:1.25:0.72:0.61.
24702917	2	29	theme	permeation	410:419	arg1	chromatography					421:434	gel permeation chromatography	406:434	gel permeation chromatography	406:434	Two EPS fractions were separated from the fermented skim milk by removing proteins, ethanol precipitation, anion-exchange and gel permeation chromatography.
24702917	2	29	theme	permeation	410:419	arg1	proteins					354:361	proteins	354:361	proteins	354:361	Two EPS fractions were separated from the fermented skim milk by removing proteins, ethanol precipitation, anion-exchange and gel permeation chromatography.
24702917	4	30	theme	acid	708:711	arg1	bacteria					713:720	lactic acid bacteria	701:720	lactic acid bacteria	701:720	Such monosaccharide composition of the exopolysaccharide by lactic acid bacteria has not been reported so far, and S1 is likely to be an unusual type of microbial EPS.
24702917	5	31	theme	molecular	849:857	arg1	weight					859:864	a high average molecular weight	834:864	a high average molecular weight of 1.23 × 10(6)Da	834:882	Whilst Fraction S2, with a high average molecular weight of 1.23 × 10(6)Da, contained rhamnose, glucose and galactose in a molar ratio of approximately 1.73:1.47:1.00.
24702917	3	32	theme	approximate	588:598	arg1	ratio					606:610	an approximate molar ratio	585:610	an approximate molar ratio of 2.03:1.29:1.25:0.72:0.61	585:638	Fraction S1, with a low average molecular weight of 1.36 × 10(4)Da, was composed of glucose, arabinose, glucosamine, galactosamine and galactose in an approximate molar ratio of 2.03:1.29:1.25:0.72:0.61.
24702917	0	33	dep	Lactobacillus	83:95	arg1	KF5					107:109	Lactobacillus rhamnosus KF5	83:109	Lactobacillus rhamnosus KF5	83:109	Partial characterization and immunostimulatory activity of exopolysaccharides from Lactobacillus rhamnosus KF5.
24702917	5	34	from	glucose	905:911	arg1	ratio					938:942	a molar ratio	930:942	a molar ratio of approximately 1.73:1.47:1.00	930:974	Whilst Fraction S2, with a high average molecular weight of 1.23 × 10(6)Da, contained rhamnose, glucose and galactose in a molar ratio of approximately 1.73:1.47:1.00.
24702917	2	35	theme	gel	406:408	arg1	chromatography					421:434	gel permeation chromatography	406:434	gel permeation chromatography	406:434	Two EPS fractions were separated from the fermented skim milk by removing proteins, ethanol precipitation, anion-exchange and gel permeation chromatography.
24702917	2	35	theme	gel	406:408	arg1	proteins					354:361	proteins	354:361	proteins	354:361	Two EPS fractions were separated from the fermented skim milk by removing proteins, ethanol precipitation, anion-exchange and gel permeation chromatography.
24702917	5	36	theme	average	841:847	arg1	weight					859:864	a high average molecular weight	834:864	a high average molecular weight of 1.23 × 10(6)Da	834:882	Whilst Fraction S2, with a high average molecular weight of 1.23 × 10(6)Da, contained rhamnose, glucose and galactose in a molar ratio of approximately 1.73:1.47:1.00.
24702917	3	37	theme	molar	600:604	arg1	ratio					606:610	an approximate molar ratio	585:610	an approximate molar ratio of 2.03:1.29:1.25:0.72:0.61	585:638	Fraction S1, with a low average molecular weight of 1.36 × 10(4)Da, was composed of glucose, arabinose, glucosamine, galactosamine and galactose in an approximate molar ratio of 2.03:1.29:1.25:0.72:0.61.
24702917	3	38	from	glucosamine	541:551	arg1	ratio					606:610	an approximate molar ratio	585:610	an approximate molar ratio of 2.03:1.29:1.25:0.72:0.61	585:638	Fraction S1, with a low average molecular weight of 1.36 × 10(4)Da, was composed of glucose, arabinose, glucosamine, galactosamine and galactose in an approximate molar ratio of 2.03:1.29:1.25:0.72:0.61.
24702917	0	39	from	Lactobacillus	83:95	arg1	exopolysaccharides					59:76	exopolysaccharides	59:76	exopolysaccharides from Lactobacillus rhamnosus KF5	59:109	Partial characterization and immunostimulatory activity of exopolysaccharides from Lactobacillus rhamnosus KF5.
24702917	0	39	from	Lactobacillus	83:95	arg1	characterization					8:23	Partial characterization	0:23	Partial characterization	0:23	Partial characterization and immunostimulatory activity of exopolysaccharides from Lactobacillus rhamnosus KF5.
24702917	0	39	from	Lactobacillus	83:95	arg1	activity					47:54	immunostimulatory activity	29:54	immunostimulatory activity	29:54	Partial characterization and immunostimulatory activity of exopolysaccharides from Lactobacillus rhamnosus KF5.
24702917	2	40	theme	skim	332:335	arg1	milk					337:340	the fermented skim milk	318:340	the fermented skim milk	318:340	Two EPS fractions were separated from the fermented skim milk by removing proteins, ethanol precipitation, anion-exchange and gel permeation chromatography.
24702917	0	41	theme	exopolysaccharides	59:76	arg1	characterization					8:23	Partial characterization	0:23	Partial characterization	0:23	Partial characterization and immunostimulatory activity of exopolysaccharides from Lactobacillus rhamnosus KF5.
24702917	0	41	theme	exopolysaccharides	59:76	arg1	activity					47:54	immunostimulatory activity	29:54	immunostimulatory activity	29:54	Partial characterization and immunostimulatory activity of exopolysaccharides from Lactobacillus rhamnosus KF5.
24702917	3	42	theme	2.03:1.29:1.25:0.72:0.61	615:638	arg1	ratio					606:610	an approximate molar ratio	585:610	an approximate molar ratio of 2.03:1.29:1.25:0.72:0.61	585:638	Fraction S1, with a low average molecular weight of 1.36 × 10(4)Da, was composed of glucose, arabinose, glucosamine, galactosamine and galactose in an approximate molar ratio of 2.03:1.29:1.25:0.72:0.61.
24702917	1	43	dep	healthy	186:192	arg1	human					194:198	human	194:198	human	194:198	Lactobacillus rhamnosus KF5, a strain newly isolated from the faeces of a healthy human volunteer, has been shown to produce the exopolysaccharides (EPS) in skim milk.
24702917	2	44	theme	fermented	322:330	arg1	milk					337:340	the fermented skim milk	318:340	the fermented skim milk	318:340	Two EPS fractions were separated from the fermented skim milk by removing proteins, ethanol precipitation, anion-exchange and gel permeation chromatography.
24702917	5	45	theme	molar	932:936	arg1	ratio					938:942	a molar ratio	930:942	a molar ratio of approximately 1.73:1.47:1.00	930:974	Whilst Fraction S2, with a high average molecular weight of 1.23 × 10(6)Da, contained rhamnose, glucose and galactose in a molar ratio of approximately 1.73:1.47:1.00.
24702917	3	46	theme	average	461:467	arg1	weight					479:484	a low average molecular weight	455:484	a low average molecular weight of 1.36 × 10(4)Da	455:502	Fraction S1, with a low average molecular weight of 1.36 × 10(4)Da, was composed of glucose, arabinose, glucosamine, galactosamine and galactose in an approximate molar ratio of 2.03:1.29:1.25:0.72:0.61.
24702917	5	47	theme	Fraction	816:823	arg1	S2					825:826	Fraction S2	816:826	Fraction S2	816:826	Whilst Fraction S2, with a high average molecular weight of 1.23 × 10(6)Da, contained rhamnose, glucose and galactose in a molar ratio of approximately 1.73:1.47:1.00.
24702917	3	48	with	S1	446:447	arg1	weight					479:484	a low average molecular weight	455:484	a low average molecular weight of 1.36 × 10(4)Da	455:502	Fraction S1, with a low average molecular weight of 1.36 × 10(4)Da, was composed of glucose, arabinose, glucosamine, galactosamine and galactose in an approximate molar ratio of 2.03:1.29:1.25:0.72:0.61.
24702917	5	49	theme	high	836:839	arg1	weight					859:864	a high average molecular weight	834:864	a high average molecular weight of 1.23 × 10(6)Da	834:882	Whilst Fraction S2, with a high average molecular weight of 1.23 × 10(6)Da, contained rhamnose, glucose and galactose in a molar ratio of approximately 1.73:1.47:1.00.
24702917	6	50	theme	immunomodulatory	1111:1126	arg1	activity					1128:1135	the potential immunomodulatory activity	1097:1135	the potential immunomodulatory activity	1097:1135	Both of EPS fractions could significantly stimulate splenocyte proliferation in vitro, indicating two EPS fractions had the potential immunomodulatory activity.
24702917	5	51	theme	1.73:1.47:1.00	961:974	arg1	ratio					938:942	a molar ratio	930:942	a molar ratio of approximately 1.73:1.47:1.00	930:974	Whilst Fraction S2, with a high average molecular weight of 1.23 × 10(6)Da, contained rhamnose, glucose and galactose in a molar ratio of approximately 1.73:1.47:1.00.
24702917	3	52	theme	molecular	469:477	arg1	weight					479:484	a low average molecular weight	455:484	a low average molecular weight of 1.36 × 10(4)Da	455:502	Fraction S1, with a low average molecular weight of 1.36 × 10(4)Da, was composed of glucose, arabinose, glucosamine, galactosamine and galactose in an approximate molar ratio of 2.03:1.29:1.25:0.72:0.61.
24702917	4	53	theme	lactic	701:706	arg1	bacteria					713:720	lactic acid bacteria	701:720	lactic acid bacteria	701:720	Such monosaccharide composition of the exopolysaccharide by lactic acid bacteria has not been reported so far, and S1 is likely to be an unusual type of microbial EPS.
24702917	6	54	theme	potential	1101:1109	arg1	activity					1128:1135	the potential immunomodulatory activity	1097:1135	the potential immunomodulatory activity	1097:1135	Both of EPS fractions could significantly stimulate splenocyte proliferation in vitro, indicating two EPS fractions had the potential immunomodulatory activity.
24702917	4	55	theme	monosaccharide	646:659	arg1	composition					661:671	Such monosaccharide composition	641:671	Such monosaccharide composition of the exopolysaccharide by lactic acid bacteria	641:720	Such monosaccharide composition of the exopolysaccharide by lactic acid bacteria has not been reported so far, and S1 is likely to be an unusual type of microbial EPS.
24702917	3	56	from	glucose	521:527	arg1	ratio					606:610	an approximate molar ratio	585:610	an approximate molar ratio of 2.03:1.29:1.25:0.72:0.61	585:638	Fraction S1, with a low average molecular weight of 1.36 × 10(4)Da, was composed of glucose, arabinose, glucosamine, galactosamine and galactose in an approximate molar ratio of 2.03:1.29:1.25:0.72:0.61.
24702917	4	57	theme	Such	641:644	arg1	composition					661:671	Such monosaccharide composition	641:671	Such monosaccharide composition of the exopolysaccharide by lactic acid bacteria	641:720	Such monosaccharide composition of the exopolysaccharide by lactic acid bacteria has not been reported so far, and S1 is likely to be an unusual type of microbial EPS.
24702917	6	58	contain	had	1093:1095	arg2	activity					1128:1135	the potential immunomodulatory activity	1097:1135	the potential immunomodulatory activity	1097:1135	Both of EPS fractions could significantly stimulate splenocyte proliferation in vitro, indicating two EPS fractions had the potential immunomodulatory activity.
24702917	6	58	contain	had	1093:1095	arg1	fractions					1083:1091	two EPS fractions	1075:1091	two EPS fractions	1075:1091	Both of EPS fractions could significantly stimulate splenocyte proliferation in vitro, indicating two EPS fractions had the potential immunomodulatory activity.
27083821	3	0	theme	PMoA/SiO2	567:575	arg1	catalyst					577:584	the reusable heterogeneous PMoA/SiO2 catalyst	540:584	the reusable heterogeneous PMoA/SiO2 catalyst	540:584	A new catalytic approach using the reusable heterogeneous PMoA/SiO2 catalyst provides benefits in terms of yields, environmental safety, operational simplicity, and thus opens new perspectives for the rational hemicellulose biomass utilization.
27083821	1	1	theme	Microwave-assisted	123:140	arg1	glycosylation					182:194	Microwave-assisted, phosphomolybdic acid (PMoA) catalyzed, glycosylation	123:194	Microwave-assisted, phosphomolybdic acid (PMoA) catalyzed, glycosylation	123:194	Microwave-assisted, phosphomolybdic acid (PMoA) catalyzed, glycosylation of unprotected d-xylose and d-lyxose, obtained after tandem Mo(VI)-catalyzed xylan hydrolysis-epimerization reaction, provides alkyl xylosides and lyxosides in short reaction times.
27083821	1	2	theme	d-lyxose	224:231	arg1	glycosylation					182:194	Microwave-assisted, phosphomolybdic acid (PMoA) catalyzed, glycosylation	123:194	Microwave-assisted, phosphomolybdic acid (PMoA) catalyzed, glycosylation	123:194	Microwave-assisted, phosphomolybdic acid (PMoA) catalyzed, glycosylation of unprotected d-xylose and d-lyxose, obtained after tandem Mo(VI)-catalyzed xylan hydrolysis-epimerization reaction, provides alkyl xylosides and lyxosides in short reaction times.
27083821	3	3	theme	yields	616:621	arg1	terms					607:611	terms	607:611	terms of yields	607:621	A new catalytic approach using the reusable heterogeneous PMoA/SiO2 catalyst provides benefits in terms of yields, environmental safety, operational simplicity, and thus opens new perspectives for the rational hemicellulose biomass utilization.
27083821	3	4	from	benefits	595:602	arg1	terms					607:611	terms	607:611	terms of yields	607:621	A new catalytic approach using the reusable heterogeneous PMoA/SiO2 catalyst provides benefits in terms of yields, environmental safety, operational simplicity, and thus opens new perspectives for the rational hemicellulose biomass utilization.
27083821	1	5	theme	alkyl	323:327	arg1	xylosides					329:337	alkyl xylosides	323:337	alkyl xylosides	323:337	Microwave-assisted, phosphomolybdic acid (PMoA) catalyzed, glycosylation of unprotected d-xylose and d-lyxose, obtained after tandem Mo(VI)-catalyzed xylan hydrolysis-epimerization reaction, provides alkyl xylosides and lyxosides in short reaction times.
27083821	2	6	theme	good	487:490	arg1	%					505:505	38-73%	500:505	38-73%	500:505	A homologous series of amphiphilic alkyl pentosides varying in chain structure (C8-C14) was prepared in very good yields (38-73%).
27083821	2	6	theme	good	487:490	arg1	yields					492:497	very good yields	482:497	very good yields (38-73%)	482:506	A homologous series of amphiphilic alkyl pentosides varying in chain structure (C8-C14) was prepared in very good yields (38-73%).
27083821	0	7	from	role	38:41	arg1	synthesis					75:83	the synthesis	71:83	the synthesis of xylan-based non-ionic surfactants	71:120	Surfactants of biological origin: The role of Mo(VI) and microwaves in the synthesis of xylan-based non-ionic surfactants.
27083821	1	8	theme	phosphomolybdic	143:157	arg1	acid					159:162	phosphomolybdic acid	143:162	phosphomolybdic acid	143:162	Microwave-assisted, phosphomolybdic acid (PMoA) catalyzed, glycosylation of unprotected d-xylose and d-lyxose, obtained after tandem Mo(VI)-catalyzed xylan hydrolysis-epimerization reaction, provides alkyl xylosides and lyxosides in short reaction times.
27083821	3	9	theme	hemicellulose	719:731	arg1	utilization					741:751	the rational hemicellulose biomass utilization	706:751	the rational hemicellulose biomass utilization	706:751	A new catalytic approach using the reusable heterogeneous PMoA/SiO2 catalyst provides benefits in terms of yields, environmental safety, operational simplicity, and thus opens new perspectives for the rational hemicellulose biomass utilization.
27083821	1	10	gly	glycosylation	182:194	arg1	d-xylose					211:218	unprotected d-xylose	199:218	unprotected d-xylose	199:218	Microwave-assisted, phosphomolybdic acid (PMoA) catalyzed, glycosylation of unprotected d-xylose and d-lyxose, obtained after tandem Mo(VI)-catalyzed xylan hydrolysis-epimerization reaction, provides alkyl xylosides and lyxosides in short reaction times.
27083821	1	10	gly	glycosylation	182:194	arg1	d-lyxose					224:231	d-lyxose	224:231	d-lyxose	224:231	Microwave-assisted, phosphomolybdic acid (PMoA) catalyzed, glycosylation of unprotected d-xylose and d-lyxose, obtained after tandem Mo(VI)-catalyzed xylan hydrolysis-epimerization reaction, provides alkyl xylosides and lyxosides in short reaction times.
27083821	1	11	dep	Microwave-assisted	123:140	arg1	catalyzed					171:179	catalyzed	171:179	catalyzed	171:179	Microwave-assisted, phosphomolybdic acid (PMoA) catalyzed, glycosylation of unprotected d-xylose and d-lyxose, obtained after tandem Mo(VI)-catalyzed xylan hydrolysis-epimerization reaction, provides alkyl xylosides and lyxosides in short reaction times.
27083821	1	12	dep	catalyzed	171:179	arg1	acid					159:162	phosphomolybdic acid	143:162	phosphomolybdic acid	143:162	Microwave-assisted, phosphomolybdic acid (PMoA) catalyzed, glycosylation of unprotected d-xylose and d-lyxose, obtained after tandem Mo(VI)-catalyzed xylan hydrolysis-epimerization reaction, provides alkyl xylosides and lyxosides in short reaction times.
27083821	3	13	theme	operational	646:656	arg1	simplicity					658:667	operational simplicity	646:667	operational simplicity	646:667	A new catalytic approach using the reusable heterogeneous PMoA/SiO2 catalyst provides benefits in terms of yields, environmental safety, operational simplicity, and thus opens new perspectives for the rational hemicellulose biomass utilization.
27083821	3	13	theme	operational	646:656	arg1	benefits					595:602	benefits	595:602	benefits in terms of yields	595:621	A new catalytic approach using the reusable heterogeneous PMoA/SiO2 catalyst provides benefits in terms of yields, environmental safety, operational simplicity, and thus opens new perspectives for the rational hemicellulose biomass utilization.
27083821	2	14	theme	pentosides	419:428	arg1	series					391:396	A homologous series	378:396	A homologous series of amphiphilic alkyl pentosides varying in chain structure (C8-C14)	378:464	A homologous series of amphiphilic alkyl pentosides varying in chain structure (C8-C14) was prepared in very good yields (38-73%).
27083821	1	15	theme	tandem	249:254	arg1	Mo					256:257	tandem Mo(VI)-catalyzed xylan hydrolysis-epimerization reaction	249:311	tandem Mo(VI)-catalyzed xylan hydrolysis-epimerization reaction	249:311	Microwave-assisted, phosphomolybdic acid (PMoA) catalyzed, glycosylation of unprotected d-xylose and d-lyxose, obtained after tandem Mo(VI)-catalyzed xylan hydrolysis-epimerization reaction, provides alkyl xylosides and lyxosides in short reaction times.
27083821	3	16	theme	catalytic	515:523	arg1	approach					525:532	A new catalytic approach	509:532	A new catalytic approach using the reusable heterogeneous PMoA/SiO2 catalyst	509:584	A new catalytic approach using the reusable heterogeneous PMoA/SiO2 catalyst provides benefits in terms of yields, environmental safety, operational simplicity, and thus opens new perspectives for the rational hemicellulose biomass utilization.
27083821	0	17	theme	origin	26:31	arg1	Surfactants					0:10	Surfactants	0:10	Surfactants of biological origin: The role of Mo(VI) and microwaves in the synthesis of xylan-based non-ionic surfactants.	0:121	Surfactants of biological origin: The role of Mo(VI) and microwaves in the synthesis of xylan-based non-ionic surfactants.
27083821	3	18	theme	rational	710:717	arg1	utilization					741:751	the rational hemicellulose biomass utilization	706:751	the rational hemicellulose biomass utilization	706:751	A new catalytic approach using the reusable heterogeneous PMoA/SiO2 catalyst provides benefits in terms of yields, environmental safety, operational simplicity, and thus opens new perspectives for the rational hemicellulose biomass utilization.
27083821	3	19	theme	new	511:513	arg1	approach					525:532	A new catalytic approach	509:532	A new catalytic approach using the reusable heterogeneous PMoA/SiO2 catalyst	509:584	A new catalytic approach using the reusable heterogeneous PMoA/SiO2 catalyst provides benefits in terms of yields, environmental safety, operational simplicity, and thus opens new perspectives for the rational hemicellulose biomass utilization.
27083821	0	20	theme	biological	15:24	arg1	origin					26:31	biological origin	15:31	biological origin	15:31	Surfactants of biological origin: The role of Mo(VI) and microwaves in the synthesis of xylan-based non-ionic surfactants.
27083821	1	21	theme	short	356:360	arg1	times					371:375	short reaction times	356:375	short reaction times	356:375	Microwave-assisted, phosphomolybdic acid (PMoA) catalyzed, glycosylation of unprotected d-xylose and d-lyxose, obtained after tandem Mo(VI)-catalyzed xylan hydrolysis-epimerization reaction, provides alkyl xylosides and lyxosides in short reaction times.
27083821	0	22	dep	Mo	46:47	arg1	VI					49:50	VI	49:50	VI	49:50	Surfactants of biological origin: The role of Mo(VI) and microwaves in the synthesis of xylan-based non-ionic surfactants.
27083821	0	23	theme	non-ionic	100:108	arg1	surfactants					110:120	xylan-based non-ionic surfactants	88:120	xylan-based non-ionic surfactants	88:120	Surfactants of biological origin: The role of Mo(VI) and microwaves in the synthesis of xylan-based non-ionic surfactants.
27083821	1	24	theme	reaction	362:369	arg1	times					371:375	short reaction times	356:375	short reaction times	356:375	Microwave-assisted, phosphomolybdic acid (PMoA) catalyzed, glycosylation of unprotected d-xylose and d-lyxose, obtained after tandem Mo(VI)-catalyzed xylan hydrolysis-epimerization reaction, provides alkyl xylosides and lyxosides in short reaction times.
27083821	2	25	theme	homologous	380:389	arg1	series					391:396	A homologous series	378:396	A homologous series of amphiphilic alkyl pentosides varying in chain structure (C8-C14)	378:464	A homologous series of amphiphilic alkyl pentosides varying in chain structure (C8-C14) was prepared in very good yields (38-73%).
27083821	0	26	theme	xylan-based	88:98	arg1	surfactants					110:120	xylan-based non-ionic surfactants	88:120	xylan-based non-ionic surfactants	88:120	Surfactants of biological origin: The role of Mo(VI) and microwaves in the synthesis of xylan-based non-ionic surfactants.
27083821	2	27	theme	alkyl	413:417	arg1	pentosides					419:428	amphiphilic alkyl pentosides	401:428	amphiphilic alkyl pentosides varying in chain structure (C8-C14)	401:464	A homologous series of amphiphilic alkyl pentosides varying in chain structure (C8-C14) was prepared in very good yields (38-73%).
27083821	3	28	theme	biomass	733:739	arg1	utilization					741:751	the rational hemicellulose biomass utilization	706:751	the rational hemicellulose biomass utilization	706:751	A new catalytic approach using the reusable heterogeneous PMoA/SiO2 catalyst provides benefits in terms of yields, environmental safety, operational simplicity, and thus opens new perspectives for the rational hemicellulose biomass utilization.
27083821	2	29	theme	chain	441:445	arg1	C8-C14					458:463	C8-C14	458:463	C8-C14	458:463	A homologous series of amphiphilic alkyl pentosides varying in chain structure (C8-C14) was prepared in very good yields (38-73%).
27083821	2	29	theme	chain	441:445	arg1	structure					447:455	chain structure	441:455	chain structure (C8-C14)	441:464	A homologous series of amphiphilic alkyl pentosides varying in chain structure (C8-C14) was prepared in very good yields (38-73%).
27083821	1	30	theme	-catalyzed	262:271	arg1	reaction					304:311	-catalyzed xylan hydrolysis-epimerization reaction	262:311	tandem Mo(VI)-catalyzed xylan hydrolysis-epimerization reaction	249:311	Microwave-assisted, phosphomolybdic acid (PMoA) catalyzed, glycosylation of unprotected d-xylose and d-lyxose, obtained after tandem Mo(VI)-catalyzed xylan hydrolysis-epimerization reaction, provides alkyl xylosides and lyxosides in short reaction times.
27083821	1	31	dep	Mo	256:257	arg1	reaction					304:311	-catalyzed xylan hydrolysis-epimerization reaction	262:311	tandem Mo(VI)-catalyzed xylan hydrolysis-epimerization reaction	249:311	Microwave-assisted, phosphomolybdic acid (PMoA) catalyzed, glycosylation of unprotected d-xylose and d-lyxose, obtained after tandem Mo(VI)-catalyzed xylan hydrolysis-epimerization reaction, provides alkyl xylosides and lyxosides in short reaction times.
27083821	1	31	dep	Mo	256:257	arg1	VI					259:260	VI	259:260	VI	259:260	Microwave-assisted, phosphomolybdic acid (PMoA) catalyzed, glycosylation of unprotected d-xylose and d-lyxose, obtained after tandem Mo(VI)-catalyzed xylan hydrolysis-epimerization reaction, provides alkyl xylosides and lyxosides in short reaction times.
27083821	1	32	theme	xylan	273:277	arg1	reaction					304:311	-catalyzed xylan hydrolysis-epimerization reaction	262:311	tandem Mo(VI)-catalyzed xylan hydrolysis-epimerization reaction	249:311	Microwave-assisted, phosphomolybdic acid (PMoA) catalyzed, glycosylation of unprotected d-xylose and d-lyxose, obtained after tandem Mo(VI)-catalyzed xylan hydrolysis-epimerization reaction, provides alkyl xylosides and lyxosides in short reaction times.
27083821	0	33	theme	surfactants	110:120	arg1	synthesis					75:83	the synthesis	71:83	the synthesis of xylan-based non-ionic surfactants	71:120	Surfactants of biological origin: The role of Mo(VI) and microwaves in the synthesis of xylan-based non-ionic surfactants.
27083821	3	34	theme	environmental	624:636	arg1	benefits					595:602	benefits	595:602	benefits in terms of yields	595:621	A new catalytic approach using the reusable heterogeneous PMoA/SiO2 catalyst provides benefits in terms of yields, environmental safety, operational simplicity, and thus opens new perspectives for the rational hemicellulose biomass utilization.
27083821	3	34	theme	environmental	624:636	arg1	safety					638:643	environmental safety	624:643	environmental safety	624:643	A new catalytic approach using the reusable heterogeneous PMoA/SiO2 catalyst provides benefits in terms of yields, environmental safety, operational simplicity, and thus opens new perspectives for the rational hemicellulose biomass utilization.
27083821	1	35	theme	hydrolysis-epimerization	279:302	arg1	reaction					304:311	-catalyzed xylan hydrolysis-epimerization reaction	262:311	tandem Mo(VI)-catalyzed xylan hydrolysis-epimerization reaction	249:311	Microwave-assisted, phosphomolybdic acid (PMoA) catalyzed, glycosylation of unprotected d-xylose and d-lyxose, obtained after tandem Mo(VI)-catalyzed xylan hydrolysis-epimerization reaction, provides alkyl xylosides and lyxosides in short reaction times.
27083821	3	36	theme	new	685:687	arg1	perspectives					689:700	new perspectives	685:700	new perspectives for the rational hemicellulose biomass utilization	685:751	A new catalytic approach using the reusable heterogeneous PMoA/SiO2 catalyst provides benefits in terms of yields, environmental safety, operational simplicity, and thus opens new perspectives for the rational hemicellulose biomass utilization.
27083821	3	37	theme	reusable	544:551	arg1	catalyst					577:584	the reusable heterogeneous PMoA/SiO2 catalyst	540:584	the reusable heterogeneous PMoA/SiO2 catalyst	540:584	A new catalytic approach using the reusable heterogeneous PMoA/SiO2 catalyst provides benefits in terms of yields, environmental safety, operational simplicity, and thus opens new perspectives for the rational hemicellulose biomass utilization.
27083821	0	38	from	microwaves	57:66	arg1	synthesis					75:83	the synthesis	71:83	the synthesis of xylan-based non-ionic surfactants	71:120	Surfactants of biological origin: The role of Mo(VI) and microwaves in the synthesis of xylan-based non-ionic surfactants.
27083821	1	39	theme	unprotected	199:209	arg1	d-xylose					211:218	unprotected d-xylose	199:218	unprotected d-xylose	199:218	Microwave-assisted, phosphomolybdic acid (PMoA) catalyzed, glycosylation of unprotected d-xylose and d-lyxose, obtained after tandem Mo(VI)-catalyzed xylan hydrolysis-epimerization reaction, provides alkyl xylosides and lyxosides in short reaction times.
27083821	3	40	theme	heterogeneous	553:565	arg1	catalyst					577:584	the reusable heterogeneous PMoA/SiO2 catalyst	540:584	the reusable heterogeneous PMoA/SiO2 catalyst	540:584	A new catalytic approach using the reusable heterogeneous PMoA/SiO2 catalyst provides benefits in terms of yields, environmental safety, operational simplicity, and thus opens new perspectives for the rational hemicellulose biomass utilization.
27083821	2	41	theme	amphiphilic	401:411	arg1	pentosides					419:428	amphiphilic alkyl pentosides	401:428	amphiphilic alkyl pentosides varying in chain structure (C8-C14)	401:464	A homologous series of amphiphilic alkyl pentosides varying in chain structure (C8-C14) was prepared in very good yields (38-73%).
27083821	1	42	theme	d-xylose	211:218	arg1	glycosylation					182:194	Microwave-assisted, phosphomolybdic acid (PMoA) catalyzed, glycosylation	123:194	Microwave-assisted, phosphomolybdic acid (PMoA) catalyzed, glycosylation	123:194	Microwave-assisted, phosphomolybdic acid (PMoA) catalyzed, glycosylation of unprotected d-xylose and d-lyxose, obtained after tandem Mo(VI)-catalyzed xylan hydrolysis-epimerization reaction, provides alkyl xylosides and lyxosides in short reaction times.
27083821	0	43	theme	Mo	46:47	arg1	microwaves					57:66	microwaves	57:66	microwaves in the synthesis of xylan-based non-ionic surfactants	57:120	Surfactants of biological origin: The role of Mo(VI) and microwaves in the synthesis of xylan-based non-ionic surfactants.
27083821	0	43	theme	Mo	46:47	arg1	role					38:41	The role	34:41	The role of Mo(VI)	34:51	Surfactants of biological origin: The role of Mo(VI) and microwaves in the synthesis of xylan-based non-ionic surfactants.
27083821	0	44	dep	Surfactants	0:10	arg1	microwaves					57:66	microwaves	57:66	microwaves in the synthesis of xylan-based non-ionic surfactants	57:120	Surfactants of biological origin: The role of Mo(VI) and microwaves in the synthesis of xylan-based non-ionic surfactants.
27083821	0	44	dep	Surfactants	0:10	arg1	role					38:41	The role	34:41	The role of Mo(VI)	34:51	Surfactants of biological origin: The role of Mo(VI) and microwaves in the synthesis of xylan-based non-ionic surfactants.
25770218	8	0	theme	polysaccharide	1188:1201	arg1	pathway					1203:1209	each polysaccharide pathway	1183:1209	each polysaccharide pathway	1183:1209	We observed that mixed biofilms inoculated with mutants containing a disruption in each polysaccharide pathway had restored mature matrix structure, composition, and biofilm drug resistance.
25770218	0	1	from	participation	10:22	arg1	assembly					42:49	biofilm matrix assembly	27:49	biofilm matrix assembly	27:49	Community participation in biofilm matrix assembly and function.
25770218	0	1	from	participation	10:22	arg1	function					55:62	function	55:62	function	55:62	Community participation in biofilm matrix assembly and function.
25770218	6	2	theme	individual	856:865	arg1	polysaccharides					874:888	the individual matrix polysaccharides	852:888	the individual matrix polysaccharides	852:888	These results indicate that matrix biogenesis entails coordinated delivery of the individual matrix polysaccharides.
25770218	7	3	theme	matrix-defective	996:1011	arg1	strains					1020:1026	matrix-defective mutant strains	996:1026	matrix-defective mutant strains	996:1026	To understand whether coordination occurs at the cellular level or the community level, we asked whether matrix-defective mutant strains could be coaxed to produce functional matrix through biofilm coculture.
25770218	8	4	from	disruption	1169:1178	arg1	pathway					1203:1209	each polysaccharide pathway	1183:1209	each polysaccharide pathway	1183:1209	We observed that mixed biofilms inoculated with mutants containing a disruption in each polysaccharide pathway had restored mature matrix structure, composition, and biofilm drug resistance.
25770218	8	5	theme	mature	1224:1229	arg1	structure					1238:1246	restored mature matrix structure	1215:1246	restored mature matrix structure	1215:1246	We observed that mixed biofilms inoculated with mutants containing a disruption in each polysaccharide pathway had restored mature matrix structure, composition, and biofilm drug resistance.
25770218	7	6	theme	mutant	1013:1018	arg1	strains					1020:1026	matrix-defective mutant strains	996:1026	matrix-defective mutant strains	996:1026	To understand whether coordination occurs at the cellular level or the community level, we asked whether matrix-defective mutant strains could be coaxed to produce functional matrix through biofilm coculture.
25770218	3	7	theme	genetic	357:363	arg1	approaches					399:408	genetic, biochemical, and pharmacological approaches	357:408	genetic, biochemical, and pharmacological approaches	357:408	Here we implement genetic, biochemical, and pharmacological approaches to unravel the contributions of these three constituents to matrix structure and function.
25770218	5	8	theme	matrix	681:686	arg1	function					688:695	matrix function	681:695	matrix function	681:695	Each of these was also required for matrix function, as assessed by assays for sequestration of the antifungal drug fluconazole.
25770218	6	9	theme	coordinated	828:838	arg1	delivery					840:847	coordinated delivery	828:847	matrix biogenesis entails coordinated delivery of the individual matrix polysaccharides	802:888	These results indicate that matrix biogenesis entails coordinated delivery of the individual matrix polysaccharides.
25770218	4	10	theme	each	610:613	arg1	concentrations					592:605	matrix concentrations	585:605	matrix concentrations of each of the other polysaccharides	585:642	Interference with synthesis or export of any one polysaccharide constituent altered matrix concentrations of each of the other polysaccharides.
25770218	5	11	theme	antifungal	745:754	arg1	fluconazole					761:771	the antifungal drug fluconazole	741:771	the antifungal drug fluconazole	741:771	Each of these was also required for matrix function, as assessed by assays for sequestration of the antifungal drug fluconazole.
25770218	6	12	dep	indicate	788:795	arg1	entails					820:826	matrix biogenesis entails	802:826	matrix biogenesis entails coordinated delivery of the individual matrix polysaccharides	802:888	These results indicate that matrix biogenesis entails coordinated delivery of the individual matrix polysaccharides.
25770218	5	13	theme	drug	756:759	arg1	fluconazole					761:771	the antifungal drug fluconazole	741:771	the antifungal drug fluconazole	741:771	Each of these was also required for matrix function, as assessed by assays for sequestration of the antifungal drug fluconazole.
25770218	9	14	theme	cooperative	1396:1406	arg1	actions					1408:1414	the cooperative actions	1392:1414	the cooperative actions of the biofilm community	1392:1439	Our results argue that functional matrix biogenesis is coordinated extracellularly and thus reflects the cooperative actions of the biofilm community.
25770218	6	15	theme	biogenesis	809:818	arg1	entails					820:826	matrix biogenesis entails	802:826	matrix biogenesis entails coordinated delivery of the individual matrix polysaccharides	802:888	These results indicate that matrix biogenesis entails coordinated delivery of the individual matrix polysaccharides.
25770218	2	16	theme	polysaccharide	263:276	arg1	constituents					278:289	major polysaccharide constituents	257:289	major polysaccharide constituents	257:289	Our prior studies indicate that the matrix is complex, with major polysaccharide constituents being α-mannan, β-1,6 glucan, and β-1,3 glucan.
25770218	1	17	theme	drug	180:183	arg1	resistance					185:194	drug resistance	180:194	drug resistance	180:194	Biofilms of the fungus Candida albicans produce extracellular matrix that confers such properties as adherence and drug resistance.
25770218	8	18	theme	mixed	1117:1121	arg1	biofilms					1123:1130	mixed biofilms	1117:1130	mixed biofilms inoculated with mutants containing a disruption in each polysaccharide pathway	1117:1209	We observed that mixed biofilms inoculated with mutants containing a disruption in each polysaccharide pathway had restored mature matrix structure, composition, and biofilm drug resistance.
25770218	6	19	theme	matrix	802:807	arg1	entails					820:826	matrix biogenesis entails	802:826	matrix biogenesis entails coordinated delivery of the individual matrix polysaccharides	802:888	These results indicate that matrix biogenesis entails coordinated delivery of the individual matrix polysaccharides.
25770218	1	20	theme	fungus	81:86	arg1	albicans					96:103	the fungus Candida albicans	77:103	the fungus Candida albicans	77:103	Biofilms of the fungus Candida albicans produce extracellular matrix that confers such properties as adherence and drug resistance.
25770218	4	21	theme	constituent	565:575	arg1	export					532:537	export	532:537	export	532:537	Interference with synthesis or export of any one polysaccharide constituent altered matrix concentrations of each of the other polysaccharides.
25770218	4	21	theme	constituent	565:575	arg1	synthesis					519:527	synthesis	519:527	synthesis	519:527	Interference with synthesis or export of any one polysaccharide constituent altered matrix concentrations of each of the other polysaccharides.
25770218	7	22	theme	community	962:970	arg1	level					972:976	the community level	958:976	the community level	958:976	To understand whether coordination occurs at the cellular level or the community level, we asked whether matrix-defective mutant strains could be coaxed to produce functional matrix through biofilm coculture.
25770218	1	23	theme	Candida	88:94	arg1	albicans					96:103	the fungus Candida albicans	77:103	the fungus Candida albicans	77:103	Biofilms of the fungus Candida albicans produce extracellular matrix that confers such properties as adherence and drug resistance.
25770218	0	24	theme	biofilm	27:33	arg1	assembly					42:49	biofilm matrix assembly	27:49	biofilm matrix assembly	27:49	Community participation in biofilm matrix assembly and function.
25770218	4	25	theme	polysaccharide	550:563	arg1	constituent					565:575	any one polysaccharide constituent	542:575	any one polysaccharide constituent	542:575	Interference with synthesis or export of any one polysaccharide constituent altered matrix concentrations of each of the other polysaccharides.
25770218	2	26	theme	β-1,6	307:311	arg1	glucan					313:318	β-1,6 glucan	307:318	β-1,6 glucan	307:318	Our prior studies indicate that the matrix is complex, with major polysaccharide constituents being α-mannan, β-1,6 glucan, and β-1,3 glucan.
25770218	6	27	dep	entails	820:826	arg1	delivery					840:847	coordinated delivery	828:847	matrix biogenesis entails coordinated delivery of the individual matrix polysaccharides	802:888	These results indicate that matrix biogenesis entails coordinated delivery of the individual matrix polysaccharides.
25770218	3	28	theme	biochemical	366:376	arg1	approaches					399:408	genetic, biochemical, and pharmacological approaches	357:408	genetic, biochemical, and pharmacological approaches	357:408	Here we implement genetic, biochemical, and pharmacological approaches to unravel the contributions of these three constituents to matrix structure and function.
25770218	4	29	theme	matrix	585:590	arg1	concentrations					592:605	matrix concentrations	585:605	matrix concentrations of each of the other polysaccharides	585:642	Interference with synthesis or export of any one polysaccharide constituent altered matrix concentrations of each of the other polysaccharides.
25770218	3	30	theme	matrix	470:475	arg1	structure					477:485	matrix structure	470:485	matrix structure	470:485	Here we implement genetic, biochemical, and pharmacological approaches to unravel the contributions of these three constituents to matrix structure and function.
25770218	8	31	theme	drug	1274:1277	arg1	resistance					1279:1288	biofilm drug resistance	1266:1288	biofilm drug resistance	1266:1288	We observed that mixed biofilms inoculated with mutants containing a disruption in each polysaccharide pathway had restored mature matrix structure, composition, and biofilm drug resistance.
25770218	9	32	theme	community	1431:1439	arg1	actions					1408:1414	the cooperative actions	1392:1414	the cooperative actions of the biofilm community	1392:1439	Our results argue that functional matrix biogenesis is coordinated extracellularly and thus reflects the cooperative actions of the biofilm community.
25770218	9	33	theme	biofilm	1423:1429	arg1	community					1431:1439	the biofilm community	1419:1439	the biofilm community	1419:1439	Our results argue that functional matrix biogenesis is coordinated extracellularly and thus reflects the cooperative actions of the biofilm community.
25770218	0	34	theme	matrix	35:40	arg1	assembly					42:49	biofilm matrix assembly	27:49	biofilm matrix assembly	27:49	Community participation in biofilm matrix assembly and function.
25770218	8	35	theme	biofilm	1266:1272	arg1	resistance					1279:1288	biofilm drug resistance	1266:1288	biofilm drug resistance	1266:1288	We observed that mixed biofilms inoculated with mutants containing a disruption in each polysaccharide pathway had restored mature matrix structure, composition, and biofilm drug resistance.
25770218	7	36	theme	functional	1055:1064	arg1	matrix					1066:1071	functional matrix	1055:1071	functional matrix	1055:1071	To understand whether coordination occurs at the cellular level or the community level, we asked whether matrix-defective mutant strains could be coaxed to produce functional matrix through biofilm coculture.
25770218	3	37	theme	constituents	454:465	arg1	contributions					425:437	the contributions	421:437	the contributions of these three constituents to matrix structure and function	421:498	Here we implement genetic, biochemical, and pharmacological approaches to unravel the contributions of these three constituents to matrix structure and function.
25770218	3	38	theme	pharmacological	383:397	arg1	approaches					399:408	genetic, biochemical, and pharmacological approaches	357:408	genetic, biochemical, and pharmacological approaches	357:408	Here we implement genetic, biochemical, and pharmacological approaches to unravel the contributions of these three constituents to matrix structure and function.
25770218	8	39	theme	matrix	1231:1236	arg1	structure					1238:1246	restored mature matrix structure	1215:1246	restored mature matrix structure	1215:1246	We observed that mixed biofilms inoculated with mutants containing a disruption in each polysaccharide pathway had restored mature matrix structure, composition, and biofilm drug resistance.
25770218	1	40	theme	extracellular	113:125	arg1	matrix					127:132	extracellular matrix	113:132	extracellular matrix that confers such properties as adherence and drug resistance	113:194	Biofilms of the fungus Candida albicans produce extracellular matrix that confers such properties as adherence and drug resistance.
25770218	9	41	theme	functional	1314:1323	arg1	biogenesis					1332:1341	functional matrix biogenesis	1314:1341	functional matrix biogenesis	1314:1341	Our results argue that functional matrix biogenesis is coordinated extracellularly and thus reflects the cooperative actions of the biofilm community.
25770218	6	42	theme	polysaccharides	874:888	arg1	delivery					840:847	coordinated delivery	828:847	matrix biogenesis entails coordinated delivery of the individual matrix polysaccharides	802:888	These results indicate that matrix biogenesis entails coordinated delivery of the individual matrix polysaccharides.
25770218	9	43	theme	matrix	1325:1330	arg1	biogenesis					1332:1341	functional matrix biogenesis	1314:1341	functional matrix biogenesis	1314:1341	Our results argue that functional matrix biogenesis is coordinated extracellularly and thus reflects the cooperative actions of the biofilm community.
25770218	6	44	theme	matrix	867:872	arg1	polysaccharides					874:888	the individual matrix polysaccharides	852:888	the individual matrix polysaccharides	852:888	These results indicate that matrix biogenesis entails coordinated delivery of the individual matrix polysaccharides.
25770218	2	45	theme	major	257:261	arg1	constituents					278:289	major polysaccharide constituents	257:289	major polysaccharide constituents	257:289	Our prior studies indicate that the matrix is complex, with major polysaccharide constituents being α-mannan, β-1,6 glucan, and β-1,3 glucan.
25770218	2	46	theme	β-1,3	325:329	arg1	glucan					331:336	β-1,3 glucan	325:336	β-1,3 glucan	325:336	Our prior studies indicate that the matrix is complex, with major polysaccharide constituents being α-mannan, β-1,6 glucan, and β-1,3 glucan.
25770218	1	47	theme	albicans	96:103	arg1	Biofilms					65:72	Biofilms	65:72	Biofilms of the fungus Candida albicans	65:103	Biofilms of the fungus Candida albicans produce extracellular matrix that confers such properties as adherence and drug resistance.
25770218	4	48	theme	other	622:626	arg1	polysaccharides					628:642	the other polysaccharides	618:642	the other polysaccharides	618:642	Interference with synthesis or export of any one polysaccharide constituent altered matrix concentrations of each of the other polysaccharides.
25770218	7	49	theme	biofilm	1081:1087	arg1	coculture					1089:1097	biofilm coculture	1081:1097	biofilm coculture	1081:1097	To understand whether coordination occurs at the cellular level or the community level, we asked whether matrix-defective mutant strains could be coaxed to produce functional matrix through biofilm coculture.
25770218	2	50	theme	prior	201:205	arg1	studies					207:213	Our prior studies	197:213	Our prior studies	197:213	Our prior studies indicate that the matrix is complex, with major polysaccharide constituents being α-mannan, β-1,6 glucan, and β-1,3 glucan.
25770218	4	51	with	Interference	501:512	arg1	export					532:537	export	532:537	export	532:537	Interference with synthesis or export of any one polysaccharide constituent altered matrix concentrations of each of the other polysaccharides.
25770218	4	51	with	Interference	501:512	arg1	synthesis					519:527	synthesis	519:527	synthesis	519:527	Interference with synthesis or export of any one polysaccharide constituent altered matrix concentrations of each of the other polysaccharides.
25770218	7	52	theme	cellular	940:947	arg1	level					949:953	the cellular level	936:953	the cellular level	936:953	To understand whether coordination occurs at the cellular level or the community level, we asked whether matrix-defective mutant strains could be coaxed to produce functional matrix through biofilm coculture.
25770218	8	53	contain	containing	1156:1165	arg2	disruption					1169:1178	a disruption	1167:1178	a disruption in each polysaccharide pathway	1167:1209	We observed that mixed biofilms inoculated with mutants containing a disruption in each polysaccharide pathway had restored mature matrix structure, composition, and biofilm drug resistance.
25770218	8	53	contain	containing	1156:1165	arg1	mutants					1148:1154	mutants	1148:1154	mutants containing a disruption in each polysaccharide pathway	1148:1209	We observed that mixed biofilms inoculated with mutants containing a disruption in each polysaccharide pathway had restored mature matrix structure, composition, and biofilm drug resistance.
25770218	5	54	theme	fluconazole	761:771	arg1	sequestration					724:736	sequestration	724:736	sequestration of the antifungal drug fluconazole	724:771	Each of these was also required for matrix function, as assessed by assays for sequestration of the antifungal drug fluconazole.
25770218	1	55	theme	such	147:150	arg1	properties					152:161	such properties	147:161	such properties	147:161	Biofilms of the fungus Candida albicans produce extracellular matrix that confers such properties as adherence and drug resistance.
25770218	8	56	contain	had	1211:1213	arg2	structure					1238:1246	restored mature matrix structure	1215:1246	restored mature matrix structure	1215:1246	We observed that mixed biofilms inoculated with mutants containing a disruption in each polysaccharide pathway had restored mature matrix structure, composition, and biofilm drug resistance.
25770218	8	56	contain	had	1211:1213	arg1	biofilms					1123:1130	mixed biofilms	1117:1130	mixed biofilms inoculated with mutants containing a disruption in each polysaccharide pathway	1117:1209	We observed that mixed biofilms inoculated with mutants containing a disruption in each polysaccharide pathway had restored mature matrix structure, composition, and biofilm drug resistance.
25770218	8	56	contain	had	1211:1213	arg2	resistance					1279:1288	biofilm drug resistance	1266:1288	biofilm drug resistance	1266:1288	We observed that mixed biofilms inoculated with mutants containing a disruption in each polysaccharide pathway had restored mature matrix structure, composition, and biofilm drug resistance.
25770218	8	56	contain	had	1211:1213	arg2	composition					1249:1259	composition	1249:1259	composition	1249:1259	We observed that mixed biofilms inoculated with mutants containing a disruption in each polysaccharide pathway had restored mature matrix structure, composition, and biofilm drug resistance.
25770218	8	57	theme	restored	1215:1222	arg1	structure					1238:1246	restored mature matrix structure	1215:1246	restored mature matrix structure	1215:1246	We observed that mixed biofilms inoculated with mutants containing a disruption in each polysaccharide pathway had restored mature matrix structure, composition, and biofilm drug resistance.
24174266	4	0	theme	human	1329:1333	arg1	line					1347:1350	a human cancer cell line	1327:1350	a human cancer cell line	1327:1350	This was shown by comparing the resulting N-glycomic profiles of the washed and low-ACN eluted fractions derived from both a human cancer cell line and an insect cell line.
24174266	2	1	theme	mannose	612:618	arg1	structures					625:634	only high mannose type structures	602:634	only high mannose type structures	602:634	An efficient method to separate these two classes of N-glycans would significantly aid in detecting the lower abundant components by MS. Capitalizing on an initial observation that only high mannose type structures were recovered in the flow-through fraction when peptide-N-glycosidase F digested peptides were passed through a C18 cartridge in 0.1% formic acid, we demonstrated here that native complex type N-glycans can be retained by C18 cartridge and to be efficiently separated from both the smaller high mannose type structures, as well as de-N-glycosylated peptides by stepwise elution with increasing ACN concentration.
24174266	2	2	theme	de-N-glycosylated	968:984	arg1	peptides					986:993	de-N-glycosylated peptides	968:993	de-N-glycosylated peptides	968:993	An efficient method to separate these two classes of N-glycans would significantly aid in detecting the lower abundant components by MS. Capitalizing on an initial observation that only high mannose type structures were recovered in the flow-through fraction when peptide-N-glycosidase F digested peptides were passed through a C18 cartridge in 0.1% formic acid, we demonstrated here that native complex type N-glycans can be retained by C18 cartridge and to be efficiently separated from both the smaller high mannose type structures, as well as de-N-glycosylated peptides by stepwise elution with increasing ACN concentration.
24174266	3	3	theme	N-glycans	1096:1104	arg1	retention					1059:1067	The weak retention	1050:1067	The weak retention of the largely hydrophilic N-glycans on C18 resin	1050:1117	The weak retention of the largely hydrophilic N-glycans on C18 resin is dependent not only on size but also increased by the presence of α6-fucosylation.
24174266	2	4	theme	stepwise	998:1005	arg1	elution					1007:1013	stepwise elution	998:1013	stepwise elution with increasing ACN concentration	998:1047	An efficient method to separate these two classes of N-glycans would significantly aid in detecting the lower abundant components by MS. Capitalizing on an initial observation that only high mannose type structures were recovered in the flow-through fraction when peptide-N-glycosidase F digested peptides were passed through a C18 cartridge in 0.1% formic acid, we demonstrated here that native complex type N-glycans can be retained by C18 cartridge and to be efficiently separated from both the smaller high mannose type structures, as well as de-N-glycosylated peptides by stepwise elution with increasing ACN concentration.
24174266	1	5	theme	N-glycomic	244:253	arg1	pool					255:258	an N-glycomic pool	241:258	an N-glycomic pool	241:258	The relative amount of high mannose structures within an N-glycomic pool differs from one source to another, but quite often it predominates over the larger size complex type structures carrying biologically important glyco-epitopes.
24174266	0	6	theme	type	70:73	arg1	N-glycans					75:83	extracting complex type N-glycans	51:83	extracting complex type N-glycans from de-N-glycosylated peptides	51:115	Facile removal of high mannose structures prior to extracting complex type N-glycans from de-N-glycosylated peptides retained by C18 solid phase to allow more efficient glycomic mapping.
24174266	2	7	dep	structures	945:954	arg1	both					910:913	both	910:913	both	910:913	An efficient method to separate these two classes of N-glycans would significantly aid in detecting the lower abundant components by MS. Capitalizing on an initial observation that only high mannose type structures were recovered in the flow-through fraction when peptide-N-glycosidase F digested peptides were passed through a C18 cartridge in 0.1% formic acid, we demonstrated here that native complex type N-glycans can be retained by C18 cartridge and to be efficiently separated from both the smaller high mannose type structures, as well as de-N-glycosylated peptides by stepwise elution with increasing ACN concentration.
24174266	0	8	theme	glycomic	169:176	arg1	mapping					178:184	more efficient glycomic mapping	154:184	more efficient glycomic mapping	154:184	Facile removal of high mannose structures prior to extracting complex type N-glycans from de-N-glycosylated peptides retained by C18 solid phase to allow more efficient glycomic mapping.
24174266	4	9	theme	eluted	1292:1297	arg1	fractions					1299:1307	the washed and low-ACN eluted fractions	1269:1307	the washed and low-ACN eluted fractions derived from both a human cancer cell line and an insect cell line	1269:1374	This was shown by comparing the resulting N-glycomic profiles of the washed and low-ACN eluted fractions derived from both a human cancer cell line and an insect cell line.
24174266	4	10	dep	eluted	1292:1297	arg1	low-ACN					1284:1290	low-ACN	1284:1290	low-ACN	1284:1290	This was shown by comparing the resulting N-glycomic profiles of the washed and low-ACN eluted fractions derived from both a human cancer cell line and an insect cell line.
24174266	0	11	theme	de-N-glycosylated	90:106	arg1	peptides					108:115	de-N-glycosylated peptides	90:115	de-N-glycosylated peptides	90:115	Facile removal of high mannose structures prior to extracting complex type N-glycans from de-N-glycosylated peptides retained by C18 solid phase to allow more efficient glycomic mapping.
24174266	3	12	theme	weak	1054:1057	arg1	retention					1059:1067	The weak retention	1050:1067	The weak retention of the largely hydrophilic N-glycans on C18 resin	1050:1117	The weak retention of the largely hydrophilic N-glycans on C18 resin is dependent not only on size but also increased by the presence of α6-fucosylation.
24174266	2	13	theme	flow-through	658:669	arg1	fraction					671:678	the flow-through fraction	654:678	the flow-through fraction	654:678	An efficient method to separate these two classes of N-glycans would significantly aid in detecting the lower abundant components by MS. Capitalizing on an initial observation that only high mannose type structures were recovered in the flow-through fraction when peptide-N-glycosidase F digested peptides were passed through a C18 cartridge in 0.1% formic acid, we demonstrated here that native complex type N-glycans can be retained by C18 cartridge and to be efficiently separated from both the smaller high mannose type structures, as well as de-N-glycosylated peptides by stepwise elution with increasing ACN concentration.
24174266	2	14	theme	lower	525:529	arg1	components					540:549	the lower abundant components	521:549	the lower abundant components by MS. Capitalizing on an initial observation that only high mannose type structures were recovered in the flow-through fraction when peptide-N-glycosidase F digested peptides were passed through a C18 cartridge in 0.1% formic acid, we demonstrated here that native complex type N-glycans can be retained by C18 cartridge and to be efficiently separated from both the smaller high mannose type structures, as well as de-N-glycosylated peptides by stepwise elution with increasing ACN concentration	521:1047	An efficient method to separate these two classes of N-glycans would significantly aid in detecting the lower abundant components by MS. Capitalizing on an initial observation that only high mannose type structures were recovered in the flow-through fraction when peptide-N-glycosidase F digested peptides were passed through a C18 cartridge in 0.1% formic acid, we demonstrated here that native complex type N-glycans can be retained by C18 cartridge and to be efficiently separated from both the smaller high mannose type structures, as well as de-N-glycosylated peptides by stepwise elution with increasing ACN concentration.
24174266	1	15	theme	larger	337:342	arg1	structures					362:371	the larger size complex type structures	333:371	the larger size complex type structures carrying biologically important glyco-epitopes	333:418	The relative amount of high mannose structures within an N-glycomic pool differs from one source to another, but quite often it predominates over the larger size complex type structures carrying biologically important glyco-epitopes.
24174266	4	16	theme	N-glycomic	1246:1255	arg1	profiles					1257:1264	the resulting N-glycomic profiles	1232:1264	the resulting N-glycomic profiles of the washed and low-ACN eluted fractions derived from both a human cancer cell line and an insect cell line	1232:1374	This was shown by comparing the resulting N-glycomic profiles of the washed and low-ACN eluted fractions derived from both a human cancer cell line and an insect cell line.
24174266	3	17	dep	size	1144:1147	arg1	only					1136:1139	only	1136:1139	only	1136:1139	The weak retention of the largely hydrophilic N-glycans on C18 resin is dependent not only on size but also increased by the presence of α6-fucosylation.
24174266	1	18	theme	size	344:347	arg1	structures					362:371	the larger size complex type structures	333:371	the larger size complex type structures carrying biologically important glyco-epitopes	333:418	The relative amount of high mannose structures within an N-glycomic pool differs from one source to another, but quite often it predominates over the larger size complex type structures carrying biologically important glyco-epitopes.
24174266	4	19	theme	resulting	1236:1244	arg1	profiles					1257:1264	the resulting N-glycomic profiles	1232:1264	the resulting N-glycomic profiles of the washed and low-ACN eluted fractions derived from both a human cancer cell line and an insect cell line	1232:1374	This was shown by comparing the resulting N-glycomic profiles of the washed and low-ACN eluted fractions derived from both a human cancer cell line and an insect cell line.
24174266	2	20	theme	formic	771:776	arg1	acid					778:781	0.1% formic acid	766:781	0.1% formic acid	766:781	An efficient method to separate these two classes of N-glycans would significantly aid in detecting the lower abundant components by MS. Capitalizing on an initial observation that only high mannose type structures were recovered in the flow-through fraction when peptide-N-glycosidase F digested peptides were passed through a C18 cartridge in 0.1% formic acid, we demonstrated here that native complex type N-glycans can be retained by C18 cartridge and to be efficiently separated from both the smaller high mannose type structures, as well as de-N-glycosylated peptides by stepwise elution with increasing ACN concentration.
24174266	1	21	theme	complex	349:355	arg1	structures					362:371	the larger size complex type structures	333:371	the larger size complex type structures carrying biologically important glyco-epitopes	333:418	The relative amount of high mannose structures within an N-glycomic pool differs from one source to another, but quite often it predominates over the larger size complex type structures carrying biologically important glyco-epitopes.
24174266	2	22	theme	N-glycans	474:482	arg1	classes					463:469	these two classes	453:469	these two classes of N-glycans	453:482	An efficient method to separate these two classes of N-glycans would significantly aid in detecting the lower abundant components by MS. Capitalizing on an initial observation that only high mannose type structures were recovered in the flow-through fraction when peptide-N-glycosidase F digested peptides were passed through a C18 cartridge in 0.1% formic acid, we demonstrated here that native complex type N-glycans can be retained by C18 cartridge and to be efficiently separated from both the smaller high mannose type structures, as well as de-N-glycosylated peptides by stepwise elution with increasing ACN concentration.
24174266	2	23	theme	digested	709:716	arg1	peptides					718:725	peptide-N-glycosidase F digested peptides	685:725	peptide-N-glycosidase F digested peptides	685:725	An efficient method to separate these two classes of N-glycans would significantly aid in detecting the lower abundant components by MS. Capitalizing on an initial observation that only high mannose type structures were recovered in the flow-through fraction when peptide-N-glycosidase F digested peptides were passed through a C18 cartridge in 0.1% formic acid, we demonstrated here that native complex type N-glycans can be retained by C18 cartridge and to be efficiently separated from both the smaller high mannose type structures, as well as de-N-glycosylated peptides by stepwise elution with increasing ACN concentration.
24174266	2	24	gly	de-N-glycosylated	968:984	arg1	peptides					986:993	de-N-glycosylated peptides	968:993	de-N-glycosylated peptides	968:993	An efficient method to separate these two classes of N-glycans would significantly aid in detecting the lower abundant components by MS. Capitalizing on an initial observation that only high mannose type structures were recovered in the flow-through fraction when peptide-N-glycosidase F digested peptides were passed through a C18 cartridge in 0.1% formic acid, we demonstrated here that native complex type N-glycans can be retained by C18 cartridge and to be efficiently separated from both the smaller high mannose type structures, as well as de-N-glycosylated peptides by stepwise elution with increasing ACN concentration.
24174266	4	25	theme	cell	1366:1369	arg1	line					1371:1374	an insect cell line	1356:1374	an insect cell line	1356:1374	This was shown by comparing the resulting N-glycomic profiles of the washed and low-ACN eluted fractions derived from both a human cancer cell line and an insect cell line.
24174266	3	26	theme	α6-fucosylation	1187:1201	arg1	presence					1175:1182	the presence	1171:1182	the presence of α6-fucosylation	1171:1201	The weak retention of the largely hydrophilic N-glycans on C18 resin is dependent not only on size but also increased by the presence of α6-fucosylation.
24174266	1	27	theme	type	357:360	arg1	structures					362:371	the larger size complex type structures	333:371	the larger size complex type structures carrying biologically important glyco-epitopes	333:418	The relative amount of high mannose structures within an N-glycomic pool differs from one source to another, but quite often it predominates over the larger size complex type structures carrying biologically important glyco-epitopes.
24174266	3	28	theme	hydrophilic	1084:1094	arg1	N-glycans					1096:1104	the largely hydrophilic N-glycans	1072:1104	the largely hydrophilic N-glycans	1072:1104	The weak retention of the largely hydrophilic N-glycans on C18 resin is dependent not only on size but also increased by the presence of α6-fucosylation.
24174266	2	29	theme	F	707:707	arg1	peptides					718:725	peptide-N-glycosidase F digested peptides	685:725	peptide-N-glycosidase F digested peptides	685:725	An efficient method to separate these two classes of N-glycans would significantly aid in detecting the lower abundant components by MS. Capitalizing on an initial observation that only high mannose type structures were recovered in the flow-through fraction when peptide-N-glycosidase F digested peptides were passed through a C18 cartridge in 0.1% formic acid, we demonstrated here that native complex type N-glycans can be retained by C18 cartridge and to be efficiently separated from both the smaller high mannose type structures, as well as de-N-glycosylated peptides by stepwise elution with increasing ACN concentration.
24174266	2	30	theme	0.1	766:768	arg1	%					769:769	%	769:769	%	769:769	An efficient method to separate these two classes of N-glycans would significantly aid in detecting the lower abundant components by MS. Capitalizing on an initial observation that only high mannose type structures were recovered in the flow-through fraction when peptide-N-glycosidase F digested peptides were passed through a C18 cartridge in 0.1% formic acid, we demonstrated here that native complex type N-glycans can be retained by C18 cartridge and to be efficiently separated from both the smaller high mannose type structures, as well as de-N-glycosylated peptides by stepwise elution with increasing ACN concentration.
24174266	1	31	contain	carrying	373:380	arg1	structures					362:371	the larger size complex type structures	333:371	the larger size complex type structures carrying biologically important glyco-epitopes	333:418	The relative amount of high mannose structures within an N-glycomic pool differs from one source to another, but quite often it predominates over the larger size complex type structures carrying biologically important glyco-epitopes.
24174266	1	31	contain	carrying	373:380	arg2	glyco-epitopes					405:418	biologically important glyco-epitopes	382:418	biologically important glyco-epitopes	382:418	The relative amount of high mannose structures within an N-glycomic pool differs from one source to another, but quite often it predominates over the larger size complex type structures carrying biologically important glyco-epitopes.
24174266	0	32	theme	Facile	0:5	arg1	removal					7:13	Facile removal	0:13	Facile removal of high mannose structures prior to extracting complex type N-glycans from de-N-glycosylated peptides	0:115	Facile removal of high mannose structures prior to extracting complex type N-glycans from de-N-glycosylated peptides retained by C18 solid phase to allow more efficient glycomic mapping.
24174266	2	33	theme	smaller	919:925	arg1	structures					945:954	the smaller high mannose type structures	915:954	the smaller high mannose type structures	915:954	An efficient method to separate these two classes of N-glycans would significantly aid in detecting the lower abundant components by MS. Capitalizing on an initial observation that only high mannose type structures were recovered in the flow-through fraction when peptide-N-glycosidase F digested peptides were passed through a C18 cartridge in 0.1% formic acid, we demonstrated here that native complex type N-glycans can be retained by C18 cartridge and to be efficiently separated from both the smaller high mannose type structures, as well as de-N-glycosylated peptides by stepwise elution with increasing ACN concentration.
24174266	2	34	theme	type	620:623	arg1	structures					625:634	only high mannose type structures	602:634	only high mannose type structures	602:634	An efficient method to separate these two classes of N-glycans would significantly aid in detecting the lower abundant components by MS. Capitalizing on an initial observation that only high mannose type structures were recovered in the flow-through fraction when peptide-N-glycosidase F digested peptides were passed through a C18 cartridge in 0.1% formic acid, we demonstrated here that native complex type N-glycans can be retained by C18 cartridge and to be efficiently separated from both the smaller high mannose type structures, as well as de-N-glycosylated peptides by stepwise elution with increasing ACN concentration.
24174266	2	35	theme	peptide-N-glycosidase	685:705	arg1	F					707:707	peptide-N-glycosidase F	685:707	peptide-N-glycosidase F digested peptides	685:725	An efficient method to separate these two classes of N-glycans would significantly aid in detecting the lower abundant components by MS. Capitalizing on an initial observation that only high mannose type structures were recovered in the flow-through fraction when peptide-N-glycosidase F digested peptides were passed through a C18 cartridge in 0.1% formic acid, we demonstrated here that native complex type N-glycans can be retained by C18 cartridge and to be efficiently separated from both the smaller high mannose type structures, as well as de-N-glycosylated peptides by stepwise elution with increasing ACN concentration.
24174266	0	36	gly	de-N-glycosylated	90:106	arg1	peptides					108:115	de-N-glycosylated peptides	90:115	de-N-glycosylated peptides	90:115	Facile removal of high mannose structures prior to extracting complex type N-glycans from de-N-glycosylated peptides retained by C18 solid phase to allow more efficient glycomic mapping.
24174266	2	37	theme	high	607:610	arg1	structures					625:634	only high mannose type structures	602:634	only high mannose type structures	602:634	An efficient method to separate these two classes of N-glycans would significantly aid in detecting the lower abundant components by MS. Capitalizing on an initial observation that only high mannose type structures were recovered in the flow-through fraction when peptide-N-glycosidase F digested peptides were passed through a C18 cartridge in 0.1% formic acid, we demonstrated here that native complex type N-glycans can be retained by C18 cartridge and to be efficiently separated from both the smaller high mannose type structures, as well as de-N-glycosylated peptides by stepwise elution with increasing ACN concentration.
24174266	0	38	theme	high	18:21	arg1	structures					31:40	high mannose structures	18:40	high mannose structures prior to extracting complex type N-glycans from de-N-glycosylated peptides	18:115	Facile removal of high mannose structures prior to extracting complex type N-glycans from de-N-glycosylated peptides retained by C18 solid phase to allow more efficient glycomic mapping.
24174266	0	39	theme	C18	129:131	arg1	phase					139:143	C18 solid phase	129:143	C18 solid phase	129:143	Facile removal of high mannose structures prior to extracting complex type N-glycans from de-N-glycosylated peptides retained by C18 solid phase to allow more efficient glycomic mapping.
24174266	1	40	theme	relative	191:198	arg1	amount					200:205	The relative amount	187:205	The relative amount of high mannose structures within an N-glycomic pool	187:258	The relative amount of high mannose structures within an N-glycomic pool differs from one source to another, but quite often it predominates over the larger size complex type structures carrying biologically important glyco-epitopes.
24174266	1	40	theme	relative	191:198	arg1	structures					223:232	high mannose structures	210:232	high mannose structures	210:232	The relative amount of high mannose structures within an N-glycomic pool differs from one source to another, but quite often it predominates over the larger size complex type structures carrying biologically important glyco-epitopes.
24174266	2	41	theme	ACN	1031:1033	arg1	concentration					1035:1047	ACN concentration	1031:1047	ACN concentration	1031:1047	An efficient method to separate these two classes of N-glycans would significantly aid in detecting the lower abundant components by MS. Capitalizing on an initial observation that only high mannose type structures were recovered in the flow-through fraction when peptide-N-glycosidase F digested peptides were passed through a C18 cartridge in 0.1% formic acid, we demonstrated here that native complex type N-glycans can be retained by C18 cartridge and to be efficiently separated from both the smaller high mannose type structures, as well as de-N-glycosylated peptides by stepwise elution with increasing ACN concentration.
24174266	0	42	theme	structures	31:40	arg1	removal					7:13	Facile removal	0:13	Facile removal of high mannose structures prior to extracting complex type N-glycans from de-N-glycosylated peptides	0:115	Facile removal of high mannose structures prior to extracting complex type N-glycans from de-N-glycosylated peptides retained by C18 solid phase to allow more efficient glycomic mapping.
24174266	0	43	theme	mannose	23:29	arg1	structures					31:40	high mannose structures	18:40	high mannose structures prior to extracting complex type N-glycans from de-N-glycosylated peptides	18:115	Facile removal of high mannose structures prior to extracting complex type N-glycans from de-N-glycosylated peptides retained by C18 solid phase to allow more efficient glycomic mapping.
24174266	3	44	from	retention	1059:1067	arg1	resin					1113:1117	C18 resin	1109:1117	C18 resin	1109:1117	The weak retention of the largely hydrophilic N-glycans on C18 resin is dependent not only on size but also increased by the presence of α6-fucosylation.
24174266	0	45	theme	solid	133:137	arg1	phase					139:143	C18 solid phase	129:143	C18 solid phase	129:143	Facile removal of high mannose structures prior to extracting complex type N-glycans from de-N-glycosylated peptides retained by C18 solid phase to allow more efficient glycomic mapping.
24174266	4	46	theme	cell	1342:1345	arg1	line					1347:1350	a human cancer cell line	1327:1350	a human cancer cell line	1327:1350	This was shown by comparing the resulting N-glycomic profiles of the washed and low-ACN eluted fractions derived from both a human cancer cell line and an insect cell line.
24174266	1	47	theme	high	210:213	arg1	structures					223:232	high mannose structures	210:232	high mannose structures	210:232	The relative amount of high mannose structures within an N-glycomic pool differs from one source to another, but quite often it predominates over the larger size complex type structures carrying biologically important glyco-epitopes.
24174266	2	48	theme	type	940:943	arg1	structures					945:954	the smaller high mannose type structures	915:954	the smaller high mannose type structures	915:954	An efficient method to separate these two classes of N-glycans would significantly aid in detecting the lower abundant components by MS. Capitalizing on an initial observation that only high mannose type structures were recovered in the flow-through fraction when peptide-N-glycosidase F digested peptides were passed through a C18 cartridge in 0.1% formic acid, we demonstrated here that native complex type N-glycans can be retained by C18 cartridge and to be efficiently separated from both the smaller high mannose type structures, as well as de-N-glycosylated peptides by stepwise elution with increasing ACN concentration.
24174266	1	49	theme	important	395:403	arg1	glyco-epitopes					405:418	biologically important glyco-epitopes	382:418	biologically important glyco-epitopes	382:418	The relative amount of high mannose structures within an N-glycomic pool differs from one source to another, but quite often it predominates over the larger size complex type structures carrying biologically important glyco-epitopes.
24174266	3	50	theme	C18	1109:1111	arg1	resin					1113:1117	C18 resin	1109:1117	C18 resin	1109:1117	The weak retention of the largely hydrophilic N-glycans on C18 resin is dependent not only on size but also increased by the presence of α6-fucosylation.
24174266	0	51	theme	prior	42:46	arg1	structures					31:40	high mannose structures	18:40	high mannose structures prior to extracting complex type N-glycans from de-N-glycosylated peptides	18:115	Facile removal of high mannose structures prior to extracting complex type N-glycans from de-N-glycosylated peptides retained by C18 solid phase to allow more efficient glycomic mapping.
24174266	2	52	theme	mannose	932:938	arg1	structures					945:954	the smaller high mannose type structures	915:954	the smaller high mannose type structures	915:954	An efficient method to separate these two classes of N-glycans would significantly aid in detecting the lower abundant components by MS. Capitalizing on an initial observation that only high mannose type structures were recovered in the flow-through fraction when peptide-N-glycosidase F digested peptides were passed through a C18 cartridge in 0.1% formic acid, we demonstrated here that native complex type N-glycans can be retained by C18 cartridge and to be efficiently separated from both the smaller high mannose type structures, as well as de-N-glycosylated peptides by stepwise elution with increasing ACN concentration.
24174266	2	53	theme	initial	577:583	arg1	observation					585:595	an initial observation that only high mannose type structures were recovered in the flow-through fraction when peptide-N-glycosidase F digested peptides were passed through a C18 cartridge in 0.1% formic acid	574:781	an initial observation that only high mannose type structures were recovered in the flow-through fraction when peptide-N-glycosidase F digested peptides were passed through a C18 cartridge in 0.1% formic acid	574:781	An efficient method to separate these two classes of N-glycans would significantly aid in detecting the lower abundant components by MS. Capitalizing on an initial observation that only high mannose type structures were recovered in the flow-through fraction when peptide-N-glycosidase F digested peptides were passed through a C18 cartridge in 0.1% formic acid, we demonstrated here that native complex type N-glycans can be retained by C18 cartridge and to be efficiently separated from both the smaller high mannose type structures, as well as de-N-glycosylated peptides by stepwise elution with increasing ACN concentration.
24174266	2	54	dep	method	434:439	arg1	separate					444:451	separate	444:451	separate	444:451	An efficient method to separate these two classes of N-glycans would significantly aid in detecting the lower abundant components by MS. Capitalizing on an initial observation that only high mannose type structures were recovered in the flow-through fraction when peptide-N-glycosidase F digested peptides were passed through a C18 cartridge in 0.1% formic acid, we demonstrated here that native complex type N-glycans can be retained by C18 cartridge and to be efficiently separated from both the smaller high mannose type structures, as well as de-N-glycosylated peptides by stepwise elution with increasing ACN concentration.
24174266	4	55	theme	fractions	1299:1307	arg1	profiles					1257:1264	the resulting N-glycomic profiles	1232:1264	the resulting N-glycomic profiles of the washed and low-ACN eluted fractions derived from both a human cancer cell line and an insect cell line	1232:1374	This was shown by comparing the resulting N-glycomic profiles of the washed and low-ACN eluted fractions derived from both a human cancer cell line and an insect cell line.
24174266	2	56	theme	type	825:828	arg1	N-glycans					830:838	native complex type N-glycans	810:838	native complex type N-glycans	810:838	An efficient method to separate these two classes of N-glycans would significantly aid in detecting the lower abundant components by MS. Capitalizing on an initial observation that only high mannose type structures were recovered in the flow-through fraction when peptide-N-glycosidase F digested peptides were passed through a C18 cartridge in 0.1% formic acid, we demonstrated here that native complex type N-glycans can be retained by C18 cartridge and to be efficiently separated from both the smaller high mannose type structures, as well as de-N-glycosylated peptides by stepwise elution with increasing ACN concentration.
24174266	2	57	theme	high	927:930	arg1	structures					945:954	the smaller high mannose type structures	915:954	the smaller high mannose type structures	915:954	An efficient method to separate these two classes of N-glycans would significantly aid in detecting the lower abundant components by MS. Capitalizing on an initial observation that only high mannose type structures were recovered in the flow-through fraction when peptide-N-glycosidase F digested peptides were passed through a C18 cartridge in 0.1% formic acid, we demonstrated here that native complex type N-glycans can be retained by C18 cartridge and to be efficiently separated from both the smaller high mannose type structures, as well as de-N-glycosylated peptides by stepwise elution with increasing ACN concentration.
24174266	0	58	theme	efficient	159:167	arg1	mapping					178:184	more efficient glycomic mapping	154:184	more efficient glycomic mapping	154:184	Facile removal of high mannose structures prior to extracting complex type N-glycans from de-N-glycosylated peptides retained by C18 solid phase to allow more efficient glycomic mapping.
24174266	0	59	from	peptides	108:115	arg1	N-glycans					75:83	extracting complex type N-glycans	51:83	extracting complex type N-glycans from de-N-glycosylated peptides	51:115	Facile removal of high mannose structures prior to extracting complex type N-glycans from de-N-glycosylated peptides retained by C18 solid phase to allow more efficient glycomic mapping.
24174266	2	60	theme	complex	817:823	arg1	N-glycans					830:838	native complex type N-glycans	810:838	native complex type N-glycans	810:838	An efficient method to separate these two classes of N-glycans would significantly aid in detecting the lower abundant components by MS. Capitalizing on an initial observation that only high mannose type structures were recovered in the flow-through fraction when peptide-N-glycosidase F digested peptides were passed through a C18 cartridge in 0.1% formic acid, we demonstrated here that native complex type N-glycans can be retained by C18 cartridge and to be efficiently separated from both the smaller high mannose type structures, as well as de-N-glycosylated peptides by stepwise elution with increasing ACN concentration.
24174266	1	61	theme	mannose	215:221	arg1	structures					223:232	high mannose structures	210:232	high mannose structures	210:232	The relative amount of high mannose structures within an N-glycomic pool differs from one source to another, but quite often it predominates over the larger size complex type structures carrying biologically important glyco-epitopes.
24174266	4	62	theme	insect	1359:1364	arg1	line					1371:1374	an insect cell line	1356:1374	an insect cell line	1356:1374	This was shown by comparing the resulting N-glycomic profiles of the washed and low-ACN eluted fractions derived from both a human cancer cell line and an insect cell line.
24174266	4	63	theme	washed	1273:1278	arg1	fractions					1299:1307	the washed and low-ACN eluted fractions	1269:1307	the washed and low-ACN eluted fractions derived from both a human cancer cell line and an insect cell line	1269:1374	This was shown by comparing the resulting N-glycomic profiles of the washed and low-ACN eluted fractions derived from both a human cancer cell line and an insect cell line.
24174266	2	64	theme	native	810:815	arg1	N-glycans					830:838	native complex type N-glycans	810:838	native complex type N-glycans	810:838	An efficient method to separate these two classes of N-glycans would significantly aid in detecting the lower abundant components by MS. Capitalizing on an initial observation that only high mannose type structures were recovered in the flow-through fraction when peptide-N-glycosidase F digested peptides were passed through a C18 cartridge in 0.1% formic acid, we demonstrated here that native complex type N-glycans can be retained by C18 cartridge and to be efficiently separated from both the smaller high mannose type structures, as well as de-N-glycosylated peptides by stepwise elution with increasing ACN concentration.
24174266	1	65	theme	structures	223:232	arg1	amount					200:205	The relative amount	187:205	The relative amount of high mannose structures within an N-glycomic pool	187:258	The relative amount of high mannose structures within an N-glycomic pool differs from one source to another, but quite often it predominates over the larger size complex type structures carrying biologically important glyco-epitopes.
24174266	1	65	theme	structures	223:232	arg1	structures					223:232	high mannose structures	210:232	high mannose structures	210:232	The relative amount of high mannose structures within an N-glycomic pool differs from one source to another, but quite often it predominates over the larger size complex type structures carrying biologically important glyco-epitopes.
24174266	0	66	theme	complex	62:68	arg1	N-glycans					75:83	extracting complex type N-glycans	51:83	extracting complex type N-glycans from de-N-glycosylated peptides	51:115	Facile removal of high mannose structures prior to extracting complex type N-glycans from de-N-glycosylated peptides retained by C18 solid phase to allow more efficient glycomic mapping.
24174266	2	67	from	cartridge	753:761	arg1	acid					778:781	0.1% formic acid	766:781	0.1% formic acid	766:781	An efficient method to separate these two classes of N-glycans would significantly aid in detecting the lower abundant components by MS. Capitalizing on an initial observation that only high mannose type structures were recovered in the flow-through fraction when peptide-N-glycosidase F digested peptides were passed through a C18 cartridge in 0.1% formic acid, we demonstrated here that native complex type N-glycans can be retained by C18 cartridge and to be efficiently separated from both the smaller high mannose type structures, as well as de-N-glycosylated peptides by stepwise elution with increasing ACN concentration.
24174266	2	68	theme	efficient	424:432	arg1	method					434:439	An efficient method	421:439	An efficient method to separate these two classes of N-glycans	421:482	An efficient method to separate these two classes of N-glycans would significantly aid in detecting the lower abundant components by MS. Capitalizing on an initial observation that only high mannose type structures were recovered in the flow-through fraction when peptide-N-glycosidase F digested peptides were passed through a C18 cartridge in 0.1% formic acid, we demonstrated here that native complex type N-glycans can be retained by C18 cartridge and to be efficiently separated from both the smaller high mannose type structures, as well as de-N-glycosylated peptides by stepwise elution with increasing ACN concentration.
24174266	4	69	attach	derived	1309:1315	arg2	fractions					1299:1307	the washed and low-ACN eluted fractions	1269:1307	the washed and low-ACN eluted fractions derived from both a human cancer cell line and an insect cell line	1269:1374	This was shown by comparing the resulting N-glycomic profiles of the washed and low-ACN eluted fractions derived from both a human cancer cell line and an insect cell line.
24174266	4	69	attach	derived	1309:1315	arg1	line					1371:1374	an insect cell line	1356:1374	an insect cell line	1356:1374	This was shown by comparing the resulting N-glycomic profiles of the washed and low-ACN eluted fractions derived from both a human cancer cell line and an insect cell line.
24174266	4	69	attach	derived	1309:1315	arg1	line					1347:1350	a human cancer cell line	1327:1350	a human cancer cell line	1327:1350	This was shown by comparing the resulting N-glycomic profiles of the washed and low-ACN eluted fractions derived from both a human cancer cell line and an insect cell line.
24174266	2	70	theme	abundant	531:538	arg1	components					540:549	the lower abundant components	521:549	the lower abundant components by MS. Capitalizing on an initial observation that only high mannose type structures were recovered in the flow-through fraction when peptide-N-glycosidase F digested peptides were passed through a C18 cartridge in 0.1% formic acid, we demonstrated here that native complex type N-glycans can be retained by C18 cartridge and to be efficiently separated from both the smaller high mannose type structures, as well as de-N-glycosylated peptides by stepwise elution with increasing ACN concentration	521:1047	An efficient method to separate these two classes of N-glycans would significantly aid in detecting the lower abundant components by MS. Capitalizing on an initial observation that only high mannose type structures were recovered in the flow-through fraction when peptide-N-glycosidase F digested peptides were passed through a C18 cartridge in 0.1% formic acid, we demonstrated here that native complex type N-glycans can be retained by C18 cartridge and to be efficiently separated from both the smaller high mannose type structures, as well as de-N-glycosylated peptides by stepwise elution with increasing ACN concentration.
24174266	2	71	theme	C18	859:861	arg1	cartridge					863:871	C18 cartridge	859:871	C18 cartridge	859:871	An efficient method to separate these two classes of N-glycans would significantly aid in detecting the lower abundant components by MS. Capitalizing on an initial observation that only high mannose type structures were recovered in the flow-through fraction when peptide-N-glycosidase F digested peptides were passed through a C18 cartridge in 0.1% formic acid, we demonstrated here that native complex type N-glycans can be retained by C18 cartridge and to be efficiently separated from both the smaller high mannose type structures, as well as de-N-glycosylated peptides by stepwise elution with increasing ACN concentration.
24174266	0	72	theme	extracting	51:60	arg1	N-glycans					75:83	extracting complex type N-glycans	51:83	extracting complex type N-glycans from de-N-glycosylated peptides	51:115	Facile removal of high mannose structures prior to extracting complex type N-glycans from de-N-glycosylated peptides retained by C18 solid phase to allow more efficient glycomic mapping.
24174266	4	73	theme	cancer	1335:1340	arg1	line					1347:1350	a human cancer cell line	1327:1350	a human cancer cell line	1327:1350	This was shown by comparing the resulting N-glycomic profiles of the washed and low-ACN eluted fractions derived from both a human cancer cell line and an insect cell line.
24174266	2	74	theme	C18	749:751	arg1	cartridge					753:761	a C18 cartridge	747:761	a C18 cartridge in 0.1% formic acid	747:781	An efficient method to separate these two classes of N-glycans would significantly aid in detecting the lower abundant components by MS. Capitalizing on an initial observation that only high mannose type structures were recovered in the flow-through fraction when peptide-N-glycosidase F digested peptides were passed through a C18 cartridge in 0.1% formic acid, we demonstrated here that native complex type N-glycans can be retained by C18 cartridge and to be efficiently separated from both the smaller high mannose type structures, as well as de-N-glycosylated peptides by stepwise elution with increasing ACN concentration.
24174266	2	75	theme	%	769:769	arg1	acid					778:781	0.1% formic acid	766:781	0.1% formic acid	766:781	An efficient method to separate these two classes of N-glycans would significantly aid in detecting the lower abundant components by MS. Capitalizing on an initial observation that only high mannose type structures were recovered in the flow-through fraction when peptide-N-glycosidase F digested peptides were passed through a C18 cartridge in 0.1% formic acid, we demonstrated here that native complex type N-glycans can be retained by C18 cartridge and to be efficiently separated from both the smaller high mannose type structures, as well as de-N-glycosylated peptides by stepwise elution with increasing ACN concentration.
28914533	4	0	theme	centers	1010:1016	arg1	configuration					981:993	the configuration	977:993	the configuration of some chiral centers in the sugar units and in the case of alginate	977:1063	Despite the near-identity of the functional units in the polysaccharide ligands, the reactivity of iron(III) hydrogels can depend on the configuration of some chiral centers in the sugar units and in the case of alginate the guluronate to mannuronate block composition, as well as pH. Here, using temperature- and field-dependent transmission Mössbauer spectroscopy, we show that the dominant iron compound detected for both the alginate and pectate gels displays features typical of a polymeric (Fe3+O6) system.
28914533	5	1	theme	indicative	1466:1475	arg1	temperature					1421:1431	temperature	1421:1431	temperature	1421:1431	The Mössbauer spectra of such systems are strongly dependent on temperature, field, size, and crystallinity, indicative of superparamagnetic relaxation of magnetically ordered nanoparticles.
28914533	4	2	theme	field-dependent	1158:1172	arg1	spectroscopy					1197:1208	temperature- and field-dependent transmission Mössbauer spectroscopy	1141:1208	temperature- and field-dependent transmission Mössbauer spectroscopy	1141:1208	Despite the near-identity of the functional units in the polysaccharide ligands, the reactivity of iron(III) hydrogels can depend on the configuration of some chiral centers in the sugar units and in the case of alginate the guluronate to mannuronate block composition, as well as pH. Here, using temperature- and field-dependent transmission Mössbauer spectroscopy, we show that the dominant iron compound detected for both the alginate and pectate gels displays features typical of a polymeric (Fe3+O6) system.
28914533	7	3	theme	results	1760:1766	arg1	implications					1738:1749	Potential biological implications	1717:1749	Potential biological implications of these results	1717:1766	Potential biological implications of these results are also discussed.
28914533	4	4	from	configuration	981:993	arg1	units					1031:1035	the sugar units	1021:1035	the sugar units	1021:1035	Despite the near-identity of the functional units in the polysaccharide ligands, the reactivity of iron(III) hydrogels can depend on the configuration of some chiral centers in the sugar units and in the case of alginate the guluronate to mannuronate block composition, as well as pH. Here, using temperature- and field-dependent transmission Mössbauer spectroscopy, we show that the dominant iron compound detected for both the alginate and pectate gels displays features typical of a polymeric (Fe3+O6) system.
28914533	4	4	from	configuration	981:993	arg1	case					1048:1051	the case	1044:1051	the case of alginate	1044:1063	Despite the near-identity of the functional units in the polysaccharide ligands, the reactivity of iron(III) hydrogels can depend on the configuration of some chiral centers in the sugar units and in the case of alginate the guluronate to mannuronate block composition, as well as pH. Here, using temperature- and field-dependent transmission Mössbauer spectroscopy, we show that the dominant iron compound detected for both the alginate and pectate gels displays features typical of a polymeric (Fe3+O6) system.
28914533	0	5	theme	Complexes	82:90	arg1	Characterization					24:39	Mössbauer Spectroscopic Characterization	0:39	Mössbauer Spectroscopic Characterization of Iron(III)-Polysaccharide Coordination Complexes: Photochemistry, Biological, and Photoresponsive Materials Implications.	0:163	Mössbauer Spectroscopic Characterization of Iron(III)-Polysaccharide Coordination Complexes: Photochemistry, Biological, and Photoresponsive Materials Implications.
28914533	3	6	theme	potential	712:720	arg1	applications					722:733	numerous potential applications	703:733	numerous potential applications	703:733	These materials have numerous potential applications in areas such as photopatternable materials, materials for controlled drug delivery, and tissue engineering.
28914533	4	7	theme	polysaccharide	901:914	arg1	ligands					916:922	the polysaccharide ligands	897:922	the polysaccharide ligands	897:922	Despite the near-identity of the functional units in the polysaccharide ligands, the reactivity of iron(III) hydrogels can depend on the configuration of some chiral centers in the sugar units and in the case of alginate the guluronate to mannuronate block composition, as well as pH. Here, using temperature- and field-dependent transmission Mössbauer spectroscopy, we show that the dominant iron compound detected for both the alginate and pectate gels displays features typical of a polymeric (Fe3+O6) system.
28914533	5	8	theme	relaxation	1498:1507	arg1	indicative					1466:1475	indicative	1466:1475	indicative	1466:1475	The Mössbauer spectra of such systems are strongly dependent on temperature, field, size, and crystallinity, indicative of superparamagnetic relaxation of magnetically ordered nanoparticles.
28914533	4	9	dep	pH.	1125:1127	arg1	show					1214:1217	show	1214:1217	show that the dominant iron compound detected for both the alginate and pectate gels displays features typical of a polymeric (Fe3+O6) system	1214:1354	Despite the near-identity of the functional units in the polysaccharide ligands, the reactivity of iron(III) hydrogels can depend on the configuration of some chiral centers in the sugar units and in the case of alginate the guluronate to mannuronate block composition, as well as pH. Here, using temperature- and field-dependent transmission Mössbauer spectroscopy, we show that the dominant iron compound detected for both the alginate and pectate gels displays features typical of a polymeric (Fe3+O6) system.
28914533	6	10	theme	nanoparticles	1634:1646	arg1	distribution					1598:1609	the size distribution	1589:1609	the size distribution of the iron oxyhydroxy nanoparticles	1589:1646	Pectate and alginate hydrogels differ in the size distribution of the iron oxyhydroxy nanoparticles, suggesting that in general smaller nanoparticles are more reactive.
28914533	5	11	theme	superparamagnetic	1480:1496	arg1	relaxation					1498:1507	superparamagnetic relaxation	1480:1507	superparamagnetic relaxation of magnetically ordered nanoparticles	1480:1545	The Mössbauer spectra of such systems are strongly dependent on temperature, field, size, and crystallinity, indicative of superparamagnetic relaxation of magnetically ordered nanoparticles.
28914533	2	12	theme	light	675:679	arg1	influence					654:662	the influence	650:662	the influence of visible light	650:679	Hence, it was somewhat surprising that a series of reports demonstrated that materials synthesized from iron(III) and polysaccharides such as alginate (poly[guluronan-co-mannuronan]) or pectate (poly[galacturonan]) formed photoresponsive materials that convert from hydrogels to sols under the influence of visible light.
28914533	4	13	theme	system	1349:1354	arg1	typical					1317:1323	typical	1317:1323	typical	1317:1323	Despite the near-identity of the functional units in the polysaccharide ligands, the reactivity of iron(III) hydrogels can depend on the configuration of some chiral centers in the sugar units and in the case of alginate the guluronate to mannuronate block composition, as well as pH. Here, using temperature- and field-dependent transmission Mössbauer spectroscopy, we show that the dominant iron compound detected for both the alginate and pectate gels displays features typical of a polymeric (Fe3+O6) system.
28914533	3	14	theme	photopatternable	752:767	arg1	materials					769:777	photopatternable materials	752:777	photopatternable materials	752:777	These materials have numerous potential applications in areas such as photopatternable materials, materials for controlled drug delivery, and tissue engineering.
28914533	5	15	theme	such	1382:1385	arg1	systems					1387:1393	such systems	1382:1393	such systems	1382:1393	The Mössbauer spectra of such systems are strongly dependent on temperature, field, size, and crystallinity, indicative of superparamagnetic relaxation of magnetically ordered nanoparticles.
28914533	2	16	theme	visible	667:673	arg1	light					675:679	visible light	667:679	visible light	667:679	Hence, it was somewhat surprising that a series of reports demonstrated that materials synthesized from iron(III) and polysaccharides such as alginate (poly[guluronan-co-mannuronan]) or pectate (poly[galacturonan]) formed photoresponsive materials that convert from hydrogels to sols under the influence of visible light.
28914533	6	17	theme	alginate	1560:1567	arg1	hydrogels					1569:1577	Pectate and alginate hydrogels	1548:1577	Pectate and alginate hydrogels	1548:1577	Pectate and alginate hydrogels differ in the size distribution of the iron oxyhydroxy nanoparticles, suggesting that in general smaller nanoparticles are more reactive.
28914533	4	18	theme	typical	1317:1323	arg1	features					1308:1315	features	1308:1315	features typical of a polymeric (Fe3+O6) system	1308:1354	Despite the near-identity of the functional units in the polysaccharide ligands, the reactivity of iron(III) hydrogels can depend on the configuration of some chiral centers in the sugar units and in the case of alginate the guluronate to mannuronate block composition, as well as pH. Here, using temperature- and field-dependent transmission Mössbauer spectroscopy, we show that the dominant iron compound detected for both the alginate and pectate gels displays features typical of a polymeric (Fe3+O6) system.
28914533	4	19	theme	units	888:892	arg1	near-identity					856:868	the near-identity	852:868	the near-identity of the functional units in the polysaccharide ligands	852:922	Despite the near-identity of the functional units in the polysaccharide ligands, the reactivity of iron(III) hydrogels can depend on the configuration of some chiral centers in the sugar units and in the case of alginate the guluronate to mannuronate block composition, as well as pH. Here, using temperature- and field-dependent transmission Mössbauer spectroscopy, we show that the dominant iron compound detected for both the alginate and pectate gels displays features typical of a polymeric (Fe3+O6) system.
28914533	6	20	theme	size	1593:1596	arg1	distribution					1598:1609	the size distribution	1589:1609	the size distribution of the iron oxyhydroxy nanoparticles	1589:1646	Pectate and alginate hydrogels differ in the size distribution of the iron oxyhydroxy nanoparticles, suggesting that in general smaller nanoparticles are more reactive.
28914533	7	21	theme	biological	1727:1736	arg1	implications					1738:1749	Potential biological implications	1717:1749	Potential biological implications of these results	1717:1766	Potential biological implications of these results are also discussed.
28914533	1	22	theme	hydroxyl-acid	192:204	arg1	complexes					206:214	hydroxyl-acid complexes	192:214	hydroxyl-acid complexes	192:214	While polycarboxylates and hydroxyl-acid complexes have long been known to be photoactive, simple carboxylate complexes which lack a significant LMCT band are not typically strongly photoactive.
28914533	6	23	theme	oxyhydroxy	1623:1632	arg1	nanoparticles					1634:1646	the iron oxyhydroxy nanoparticles	1614:1646	the iron oxyhydroxy nanoparticles	1614:1646	Pectate and alginate hydrogels differ in the size distribution of the iron oxyhydroxy nanoparticles, suggesting that in general smaller nanoparticles are more reactive.
28914533	4	24	theme	polymeric	1330:1338	arg1	system					1349:1354	a polymeric (Fe3+O6) system	1328:1354	a polymeric (Fe3+O6) system	1328:1354	Despite the near-identity of the functional units in the polysaccharide ligands, the reactivity of iron(III) hydrogels can depend on the configuration of some chiral centers in the sugar units and in the case of alginate the guluronate to mannuronate block composition, as well as pH. Here, using temperature- and field-dependent transmission Mössbauer spectroscopy, we show that the dominant iron compound detected for both the alginate and pectate gels displays features typical of a polymeric (Fe3+O6) system.
28914533	4	25	theme	functional	877:886	arg1	units					888:892	the functional units	873:892	the functional units in the polysaccharide ligands	873:922	Despite the near-identity of the functional units in the polysaccharide ligands, the reactivity of iron(III) hydrogels can depend on the configuration of some chiral centers in the sugar units and in the case of alginate the guluronate to mannuronate block composition, as well as pH. Here, using temperature- and field-dependent transmission Mössbauer spectroscopy, we show that the dominant iron compound detected for both the alginate and pectate gels displays features typical of a polymeric (Fe3+O6) system.
28914533	6	26	from	reactive	1707:1714	arg1	general					1668:1674	general	1668:1674	general	1668:1674	Pectate and alginate hydrogels differ in the size distribution of the iron oxyhydroxy nanoparticles, suggesting that in general smaller nanoparticles are more reactive.
28914533	6	27	theme	Pectate	1548:1554	arg1	hydrogels					1569:1577	Pectate and alginate hydrogels	1548:1577	Pectate and alginate hydrogels	1548:1577	Pectate and alginate hydrogels differ in the size distribution of the iron oxyhydroxy nanoparticles, suggesting that in general smaller nanoparticles are more reactive.
28914533	0	28	theme	Spectroscopic	10:22	arg1	Characterization					24:39	Mössbauer Spectroscopic Characterization	0:39	Mössbauer Spectroscopic Characterization of Iron(III)-Polysaccharide Coordination Complexes: Photochemistry, Biological, and Photoresponsive Materials Implications.	0:163	Mössbauer Spectroscopic Characterization of Iron(III)-Polysaccharide Coordination Complexes: Photochemistry, Biological, and Photoresponsive Materials Implications.
28914533	4	29	theme	chiral	1003:1008	arg1	centers					1010:1016	some chiral centers	998:1016	some chiral centers in the sugar units and in the case of alginate	998:1063	Despite the near-identity of the functional units in the polysaccharide ligands, the reactivity of iron(III) hydrogels can depend on the configuration of some chiral centers in the sugar units and in the case of alginate the guluronate to mannuronate block composition, as well as pH. Here, using temperature- and field-dependent transmission Mössbauer spectroscopy, we show that the dominant iron compound detected for both the alginate and pectate gels displays features typical of a polymeric (Fe3+O6) system.
28914533	4	30	from	case	1048:1051	arg1	configuration					981:993	the configuration	977:993	the configuration of some chiral centers in the sugar units and in the case of alginate	977:1063	Despite the near-identity of the functional units in the polysaccharide ligands, the reactivity of iron(III) hydrogels can depend on the configuration of some chiral centers in the sugar units and in the case of alginate the guluronate to mannuronate block composition, as well as pH. Here, using temperature- and field-dependent transmission Mössbauer spectroscopy, we show that the dominant iron compound detected for both the alginate and pectate gels displays features typical of a polymeric (Fe3+O6) system.
28914533	6	31	from	general	1668:1674	arg1	reactive					1707:1714	reactive	1707:1714	reactive	1707:1714	Pectate and alginate hydrogels differ in the size distribution of the iron oxyhydroxy nanoparticles, suggesting that in general smaller nanoparticles are more reactive.
28914533	0	32	theme	Mössbauer	0:8	arg1	Characterization					24:39	Mössbauer Spectroscopic Characterization	0:39	Mössbauer Spectroscopic Characterization of Iron(III)-Polysaccharide Coordination Complexes: Photochemistry, Biological, and Photoresponsive Materials Implications.	0:163	Mössbauer Spectroscopic Characterization of Iron(III)-Polysaccharide Coordination Complexes: Photochemistry, Biological, and Photoresponsive Materials Implications.
28914533	1	33	theme	significant	298:308	arg1	band					315:318	a significant LMCT band	296:318	a significant LMCT band	296:318	While polycarboxylates and hydroxyl-acid complexes have long been known to be photoactive, simple carboxylate complexes which lack a significant LMCT band are not typically strongly photoactive.
28914533	6	34	theme	smaller	1676:1682	arg1	nanoparticles					1684:1696	smaller nanoparticles	1676:1696	smaller nanoparticles	1676:1696	Pectate and alginate hydrogels differ in the size distribution of the iron oxyhydroxy nanoparticles, suggesting that in general smaller nanoparticles are more reactive.
28914533	4	35	theme	dominant	1228:1235	arg1	compound					1242:1249	the dominant iron compound	1224:1249	the dominant iron compound detected for both the alginate and pectate gels	1224:1297	Despite the near-identity of the functional units in the polysaccharide ligands, the reactivity of iron(III) hydrogels can depend on the configuration of some chiral centers in the sugar units and in the case of alginate the guluronate to mannuronate block composition, as well as pH. Here, using temperature- and field-dependent transmission Mössbauer spectroscopy, we show that the dominant iron compound detected for both the alginate and pectate gels displays features typical of a polymeric (Fe3+O6) system.
28914533	5	36	theme	nanoparticles	1533:1545	arg1	relaxation					1498:1507	superparamagnetic relaxation	1480:1507	superparamagnetic relaxation of magnetically ordered nanoparticles	1480:1545	The Mössbauer spectra of such systems are strongly dependent on temperature, field, size, and crystallinity, indicative of superparamagnetic relaxation of magnetically ordered nanoparticles.
28914533	2	37	theme	reports	411:417	arg1	series					401:406	a series	399:406	a series of reports	399:417	Hence, it was somewhat surprising that a series of reports demonstrated that materials synthesized from iron(III) and polysaccharides such as alginate (poly[guluronan-co-mannuronan]) or pectate (poly[galacturonan]) formed photoresponsive materials that convert from hydrogels to sols under the influence of visible light.
28914533	3	38	contain	have	698:701	arg1	materials					688:696	These materials	682:696	These materials	682:696	These materials have numerous potential applications in areas such as photopatternable materials, materials for controlled drug delivery, and tissue engineering.
28914533	3	38	contain	have	698:701	arg2	applications					722:733	numerous potential applications	703:733	numerous potential applications	703:733	These materials have numerous potential applications in areas such as photopatternable materials, materials for controlled drug delivery, and tissue engineering.
28914533	1	39	theme	LMCT	310:313	arg1	band					315:318	a significant LMCT band	296:318	a significant LMCT band	296:318	While polycarboxylates and hydroxyl-acid complexes have long been known to be photoactive, simple carboxylate complexes which lack a significant LMCT band are not typically strongly photoactive.
28914533	0	40	theme	Photoresponsive	125:139	arg1	Implications					151:162	Photochemistry, Biological, and Photoresponsive Materials Implications	93:162	Implications	151:162	Mössbauer Spectroscopic Characterization of Iron(III)-Polysaccharide Coordination Complexes: Photochemistry, Biological, and Photoresponsive Materials Implications.
28914533	6	41	theme	iron	1618:1621	arg1	nanoparticles					1634:1646	the iron oxyhydroxy nanoparticles	1614:1646	the iron oxyhydroxy nanoparticles	1614:1646	Pectate and alginate hydrogels differ in the size distribution of the iron oxyhydroxy nanoparticles, suggesting that in general smaller nanoparticles are more reactive.
28914533	5	42	theme	systems	1387:1393	arg1	spectra					1371:1377	The Mössbauer spectra	1357:1377	The Mössbauer spectra of such systems	1357:1393	The Mössbauer spectra of such systems are strongly dependent on temperature, field, size, and crystallinity, indicative of superparamagnetic relaxation of magnetically ordered nanoparticles.
28914533	5	42	theme	systems	1387:1393	arg1	dependent					1408:1416	dependent	1408:1416	dependent	1408:1416	The Mössbauer spectra of such systems are strongly dependent on temperature, field, size, and crystallinity, indicative of superparamagnetic relaxation of magnetically ordered nanoparticles.
28914533	4	43	theme	hydrogels	953:961	arg1	reactivity					929:938	the reactivity	925:938	the reactivity of iron(III) hydrogels	925:961	Despite the near-identity of the functional units in the polysaccharide ligands, the reactivity of iron(III) hydrogels can depend on the configuration of some chiral centers in the sugar units and in the case of alginate the guluronate to mannuronate block composition, as well as pH. Here, using temperature- and field-dependent transmission Mössbauer spectroscopy, we show that the dominant iron compound detected for both the alginate and pectate gels displays features typical of a polymeric (Fe3+O6) system.
28914533	4	44	from	units	888:892	arg1	ligands					916:922	the polysaccharide ligands	897:922	the polysaccharide ligands	897:922	Despite the near-identity of the functional units in the polysaccharide ligands, the reactivity of iron(III) hydrogels can depend on the configuration of some chiral centers in the sugar units and in the case of alginate the guluronate to mannuronate block composition, as well as pH. Here, using temperature- and field-dependent transmission Mössbauer spectroscopy, we show that the dominant iron compound detected for both the alginate and pectate gels displays features typical of a polymeric (Fe3+O6) system.
28914533	5	45	theme	ordered	1525:1531	arg1	nanoparticles					1533:1545	magnetically ordered nanoparticles	1512:1545	magnetically ordered nanoparticles	1512:1545	The Mössbauer spectra of such systems are strongly dependent on temperature, field, size, and crystallinity, indicative of superparamagnetic relaxation of magnetically ordered nanoparticles.
28914533	4	46	theme	pectate	1286:1292	arg1	gels					1294:1297	both the alginate and pectate gels	1264:1297	gels	1294:1297	Despite the near-identity of the functional units in the polysaccharide ligands, the reactivity of iron(III) hydrogels can depend on the configuration of some chiral centers in the sugar units and in the case of alginate the guluronate to mannuronate block composition, as well as pH. Here, using temperature- and field-dependent transmission Mössbauer spectroscopy, we show that the dominant iron compound detected for both the alginate and pectate gels displays features typical of a polymeric (Fe3+O6) system.
28914533	0	47	theme	Iron	44:47	arg1	Complexes					82:90	Iron(III)-Polysaccharide Coordination Complexes	44:90	Iron(III)-Polysaccharide Coordination Complexes	44:90	Mössbauer Spectroscopic Characterization of Iron(III)-Polysaccharide Coordination Complexes: Photochemistry, Biological, and Photoresponsive Materials Implications.
28914533	4	48	from	units	1031:1035	arg1	configuration					981:993	the configuration	977:993	the configuration of some chiral centers in the sugar units and in the case of alginate	977:1063	Despite the near-identity of the functional units in the polysaccharide ligands, the reactivity of iron(III) hydrogels can depend on the configuration of some chiral centers in the sugar units and in the case of alginate the guluronate to mannuronate block composition, as well as pH. Here, using temperature- and field-dependent transmission Mössbauer spectroscopy, we show that the dominant iron compound detected for both the alginate and pectate gels displays features typical of a polymeric (Fe3+O6) system.
28914533	0	49	theme	Materials	141:149	arg1	Implications					151:162	Photochemistry, Biological, and Photoresponsive Materials Implications	93:162	Implications	151:162	Mössbauer Spectroscopic Characterization of Iron(III)-Polysaccharide Coordination Complexes: Photochemistry, Biological, and Photoresponsive Materials Implications.
28914533	1	50	theme	simple	256:261	arg1	complexes					275:283	simple carboxylate complexes	256:283	simple carboxylate complexes which lack a significant LMCT band	256:318	While polycarboxylates and hydroxyl-acid complexes have long been known to be photoactive, simple carboxylate complexes which lack a significant LMCT band are not typically strongly photoactive.
28914533	7	51	theme	Potential	1717:1725	arg1	implications					1738:1749	Potential biological implications	1717:1749	Potential biological implications of these results	1717:1766	Potential biological implications of these results are also discussed.
28914533	3	52	theme	controlled	794:803	arg1	delivery					810:817	controlled drug delivery	794:817	controlled drug delivery	794:817	These materials have numerous potential applications in areas such as photopatternable materials, materials for controlled drug delivery, and tissue engineering.
28914533	4	53	theme	Mössbauer	1187:1195	arg1	spectroscopy					1197:1208	temperature- and field-dependent transmission Mössbauer spectroscopy	1141:1208	temperature- and field-dependent transmission Mössbauer spectroscopy	1141:1208	Despite the near-identity of the functional units in the polysaccharide ligands, the reactivity of iron(III) hydrogels can depend on the configuration of some chiral centers in the sugar units and in the case of alginate the guluronate to mannuronate block composition, as well as pH. Here, using temperature- and field-dependent transmission Mössbauer spectroscopy, we show that the dominant iron compound detected for both the alginate and pectate gels displays features typical of a polymeric (Fe3+O6) system.
28914533	4	54	from	ligands	916:922	arg1	near-identity					856:868	the near-identity	852:868	the near-identity of the functional units in the polysaccharide ligands	852:922	Despite the near-identity of the functional units in the polysaccharide ligands, the reactivity of iron(III) hydrogels can depend on the configuration of some chiral centers in the sugar units and in the case of alginate the guluronate to mannuronate block composition, as well as pH. Here, using temperature- and field-dependent transmission Mössbauer spectroscopy, we show that the dominant iron compound detected for both the alginate and pectate gels displays features typical of a polymeric (Fe3+O6) system.
28914533	4	55	theme	sugar	1025:1029	arg1	units					1031:1035	the sugar units	1021:1035	the sugar units	1021:1035	Despite the near-identity of the functional units in the polysaccharide ligands, the reactivity of iron(III) hydrogels can depend on the configuration of some chiral centers in the sugar units and in the case of alginate the guluronate to mannuronate block composition, as well as pH. Here, using temperature- and field-dependent transmission Mössbauer spectroscopy, we show that the dominant iron compound detected for both the alginate and pectate gels displays features typical of a polymeric (Fe3+O6) system.
28914533	3	56	theme	drug	805:808	arg1	delivery					810:817	controlled drug delivery	794:817	controlled drug delivery	794:817	These materials have numerous potential applications in areas such as photopatternable materials, materials for controlled drug delivery, and tissue engineering.
28914533	4	57	from	near-identity	856:868	arg1	ligands					916:922	the polysaccharide ligands	897:922	the polysaccharide ligands	897:922	Despite the near-identity of the functional units in the polysaccharide ligands, the reactivity of iron(III) hydrogels can depend on the configuration of some chiral centers in the sugar units and in the case of alginate the guluronate to mannuronate block composition, as well as pH. Here, using temperature- and field-dependent transmission Mössbauer spectroscopy, we show that the dominant iron compound detected for both the alginate and pectate gels displays features typical of a polymeric (Fe3+O6) system.
28914533	2	58	theme	photoresponsive	582:596	arg1	materials					598:606	photoresponsive materials	582:606	photoresponsive materials that convert from hydrogels to sols under the influence of visible light	582:679	Hence, it was somewhat surprising that a series of reports demonstrated that materials synthesized from iron(III) and polysaccharides such as alginate (poly[guluronan-co-mannuronan]) or pectate (poly[galacturonan]) formed photoresponsive materials that convert from hydrogels to sols under the influence of visible light.
28914533	3	59	theme	numerous	703:710	arg1	applications					722:733	numerous potential applications	703:733	numerous potential applications	703:733	These materials have numerous potential applications in areas such as photopatternable materials, materials for controlled drug delivery, and tissue engineering.
28914533	4	60	theme	iron	1237:1240	arg1	compound					1242:1249	the dominant iron compound	1224:1249	the dominant iron compound detected for both the alginate and pectate gels	1224:1297	Despite the near-identity of the functional units in the polysaccharide ligands, the reactivity of iron(III) hydrogels can depend on the configuration of some chiral centers in the sugar units and in the case of alginate the guluronate to mannuronate block composition, as well as pH. Here, using temperature- and field-dependent transmission Mössbauer spectroscopy, we show that the dominant iron compound detected for both the alginate and pectate gels displays features typical of a polymeric (Fe3+O6) system.
28914533	5	61	theme	Mössbauer	1361:1369	arg1	spectra					1371:1377	The Mössbauer spectra	1357:1377	The Mössbauer spectra of such systems	1357:1393	The Mössbauer spectra of such systems are strongly dependent on temperature, field, size, and crystallinity, indicative of superparamagnetic relaxation of magnetically ordered nanoparticles.
28914533	5	61	theme	Mössbauer	1361:1369	arg1	dependent					1408:1416	dependent	1408:1416	dependent	1408:1416	The Mössbauer spectra of such systems are strongly dependent on temperature, field, size, and crystallinity, indicative of superparamagnetic relaxation of magnetically ordered nanoparticles.
28914533	4	62	from	centers	1010:1016	arg1	units					1031:1035	the sugar units	1021:1035	the sugar units	1021:1035	Despite the near-identity of the functional units in the polysaccharide ligands, the reactivity of iron(III) hydrogels can depend on the configuration of some chiral centers in the sugar units and in the case of alginate the guluronate to mannuronate block composition, as well as pH. Here, using temperature- and field-dependent transmission Mössbauer spectroscopy, we show that the dominant iron compound detected for both the alginate and pectate gels displays features typical of a polymeric (Fe3+O6) system.
28914533	4	62	from	centers	1010:1016	arg1	case					1048:1051	the case	1044:1051	the case of alginate	1044:1063	Despite the near-identity of the functional units in the polysaccharide ligands, the reactivity of iron(III) hydrogels can depend on the configuration of some chiral centers in the sugar units and in the case of alginate the guluronate to mannuronate block composition, as well as pH. Here, using temperature- and field-dependent transmission Mössbauer spectroscopy, we show that the dominant iron compound detected for both the alginate and pectate gels displays features typical of a polymeric (Fe3+O6) system.
28914533	4	63	theme	iron	943:946	arg1	hydrogels					953:961	iron(III) hydrogels	943:961	iron(III) hydrogels	943:961	Despite the near-identity of the functional units in the polysaccharide ligands, the reactivity of iron(III) hydrogels can depend on the configuration of some chiral centers in the sugar units and in the case of alginate the guluronate to mannuronate block composition, as well as pH. Here, using temperature- and field-dependent transmission Mössbauer spectroscopy, we show that the dominant iron compound detected for both the alginate and pectate gels displays features typical of a polymeric (Fe3+O6) system.
28914533	0	64	dep	Characterization	24:39	arg1	Implications					151:162	Photochemistry, Biological, and Photoresponsive Materials Implications	93:162	Implications	151:162	Mössbauer Spectroscopic Characterization of Iron(III)-Polysaccharide Coordination Complexes: Photochemistry, Biological, and Photoresponsive Materials Implications.
28914533	0	64	dep	Characterization	24:39	arg1	Photochemistry					93:106	Photochemistry	93:106	Photochemistry	93:106	Mössbauer Spectroscopic Characterization of Iron(III)-Polysaccharide Coordination Complexes: Photochemistry, Biological, and Photoresponsive Materials Implications.
28914533	0	65	theme	Coordination	69:80	arg1	Complexes					82:90	Iron(III)-Polysaccharide Coordination Complexes	44:90	Iron(III)-Polysaccharide Coordination Complexes	44:90	Mössbauer Spectroscopic Characterization of Iron(III)-Polysaccharide Coordination Complexes: Photochemistry, Biological, and Photoresponsive Materials Implications.
28914533	4	66	theme	alginate	1273:1280	arg1	gels					1294:1297	both the alginate and pectate gels	1264:1297	gels	1294:1297	Despite the near-identity of the functional units in the polysaccharide ligands, the reactivity of iron(III) hydrogels can depend on the configuration of some chiral centers in the sugar units and in the case of alginate the guluronate to mannuronate block composition, as well as pH. Here, using temperature- and field-dependent transmission Mössbauer spectroscopy, we show that the dominant iron compound detected for both the alginate and pectate gels displays features typical of a polymeric (Fe3+O6) system.
28914533	4	67	theme	temperature-	1141:1152	arg1	spectroscopy					1197:1208	temperature- and field-dependent transmission Mössbauer spectroscopy	1141:1208	temperature- and field-dependent transmission Mössbauer spectroscopy	1141:1208	Despite the near-identity of the functional units in the polysaccharide ligands, the reactivity of iron(III) hydrogels can depend on the configuration of some chiral centers in the sugar units and in the case of alginate the guluronate to mannuronate block composition, as well as pH. Here, using temperature- and field-dependent transmission Mössbauer spectroscopy, we show that the dominant iron compound detected for both the alginate and pectate gels displays features typical of a polymeric (Fe3+O6) system.
28914533	4	68	theme	block	1095:1099	arg1	composition					1101:1111	block composition	1095:1111	block composition	1095:1111	Despite the near-identity of the functional units in the polysaccharide ligands, the reactivity of iron(III) hydrogels can depend on the configuration of some chiral centers in the sugar units and in the case of alginate the guluronate to mannuronate block composition, as well as pH. Here, using temperature- and field-dependent transmission Mössbauer spectroscopy, we show that the dominant iron compound detected for both the alginate and pectate gels displays features typical of a polymeric (Fe3+O6) system.
28914533	3	69	theme	tissue	824:829	arg1	engineering					831:841	tissue engineering	824:841	tissue engineering	824:841	These materials have numerous potential applications in areas such as photopatternable materials, materials for controlled drug delivery, and tissue engineering.
28914533	0	70	theme	-Polysaccharide	53:67	arg1	Complexes					82:90	Iron(III)-Polysaccharide Coordination Complexes	44:90	Iron(III)-Polysaccharide Coordination Complexes	44:90	Mössbauer Spectroscopic Characterization of Iron(III)-Polysaccharide Coordination Complexes: Photochemistry, Biological, and Photoresponsive Materials Implications.
28914533	4	71	theme	Fe3+O6	1341:1346	arg1	system					1349:1354	a polymeric (Fe3+O6) system	1328:1354	a polymeric (Fe3+O6) system	1328:1354	Despite the near-identity of the functional units in the polysaccharide ligands, the reactivity of iron(III) hydrogels can depend on the configuration of some chiral centers in the sugar units and in the case of alginate the guluronate to mannuronate block composition, as well as pH. Here, using temperature- and field-dependent transmission Mössbauer spectroscopy, we show that the dominant iron compound detected for both the alginate and pectate gels displays features typical of a polymeric (Fe3+O6) system.
28914533	4	72	theme	transmission	1174:1185	arg1	spectroscopy					1197:1208	temperature- and field-dependent transmission Mössbauer spectroscopy	1141:1208	temperature- and field-dependent transmission Mössbauer spectroscopy	1141:1208	Despite the near-identity of the functional units in the polysaccharide ligands, the reactivity of iron(III) hydrogels can depend on the configuration of some chiral centers in the sugar units and in the case of alginate the guluronate to mannuronate block composition, as well as pH. Here, using temperature- and field-dependent transmission Mössbauer spectroscopy, we show that the dominant iron compound detected for both the alginate and pectate gels displays features typical of a polymeric (Fe3+O6) system.
28914533	4	73	theme	alginate	1056:1063	arg1	case					1048:1051	the case	1044:1051	the case of alginate	1044:1063	Despite the near-identity of the functional units in the polysaccharide ligands, the reactivity of iron(III) hydrogels can depend on the configuration of some chiral centers in the sugar units and in the case of alginate the guluronate to mannuronate block composition, as well as pH. Here, using temperature- and field-dependent transmission Mössbauer spectroscopy, we show that the dominant iron compound detected for both the alginate and pectate gels displays features typical of a polymeric (Fe3+O6) system.
28914533	1	74	theme	carboxylate	263:273	arg1	complexes					275:283	simple carboxylate complexes	256:283	simple carboxylate complexes which lack a significant LMCT band	256:318	While polycarboxylates and hydroxyl-acid complexes have long been known to be photoactive, simple carboxylate complexes which lack a significant LMCT band are not typically strongly photoactive.
27333892	0	0	theme	coat	56:59	arg1	mucilage					61:68	seed coat mucilage	51:68	seed coat mucilage	51:68	Irregular xylem 7 (IRX7) is required for anchoring seed coat mucilage in Arabidopsis.
27333892	11	1	theme	mucilage	1620:1627	arg1	property					1598:1605	the adhesive property	1585:1605	the adhesive property of seed coat mucilage	1585:1627	Taken together, these results indicate that xylan synthesized by IRX7 plays an essential role in maintaining the adhesive property of seed coat mucilage, and its structural role is potentially implemented through its interaction with cellulose.
27333892	8	2	theme	present	1178:1184	arg1	components					1167:1176	the higher molecular weight polymer components	1131:1176	the higher molecular weight polymer components present in the WT	1131:1194	The polymeric structure of water-soluble mucilage was altered in irx7, with a total loss of the higher molecular weight polymer components present in the WT.
27333892	7	3	theme	xylose	896:901	arg1	xylose					896:901	xylose	896:901	xylose	896:901	Both the amount of xylose and the extent of glycosyl linkages of xylan was dramatically decreased in irx7 water-soluble and adherent mucilage compared to the WT.
27333892	7	3	theme	xylose	896:901	arg1	extent					911:916	the extent	907:916	the extent of glycosyl linkages of xylan	907:946	Both the amount of xylose and the extent of glycosyl linkages of xylan was dramatically decreased in irx7 water-soluble and adherent mucilage compared to the WT.
27333892	7	3	theme	xylose	896:901	arg1	amount					886:891	the amount	882:891	the amount of xylose	882:901	Both the amount of xylose and the extent of glycosyl linkages of xylan was dramatically decreased in irx7 water-soluble and adherent mucilage compared to the WT.
27333892	7	3	theme	xylose	896:901	arg1	linkages					930:937	glycosyl linkages	921:937	glycosyl linkages of xylan	921:946	Both the amount of xylose and the extent of glycosyl linkages of xylan was dramatically decreased in irx7 water-soluble and adherent mucilage compared to the WT.
27333892	9	4	theme	extracted	1271:1279	arg1	mucilage					1281:1288	extracted mucilage	1271:1288	extracted mucilage	1271:1288	Correspondingly, whole-seed immunolabeling assays and dot-immunoassays of extracted mucilage indicated dramatic changes in rhamnogalacturonan I (RG I) and xylan epitopes in irx7 mucilage.
27333892	0	5	theme	seed	51:54	arg1	mucilage					61:68	seed coat mucilage	51:68	seed coat mucilage	51:68	Irregular xylem 7 (IRX7) is required for anchoring seed coat mucilage in Arabidopsis.
27333892	7	6	theme	irx7	978:981	arg1	mucilage					1010:1017	irx7 water-soluble and adherent mucilage	978:1017	irx7 water-soluble and adherent mucilage	978:1017	Both the amount of xylose and the extent of glycosyl linkages of xylan was dramatically decreased in irx7 water-soluble and adherent mucilage compared to the WT.
27333892	1	7	theme	seed	134:137	arg1	cells					154:158	seed coat epidermis cells	134:158	seed coat epidermis cells	134:158	Large quantities of mucilage are synthesized in seed coat epidermis cells during seed coat differentiation.
27333892	9	8	theme	mucilage	1281:1288	arg1	dot-immunoassays					1251:1266	dot-immunoassays	1251:1266	dot-immunoassays of extracted mucilage	1251:1288	Correspondingly, whole-seed immunolabeling assays and dot-immunoassays of extracted mucilage indicated dramatic changes in rhamnogalacturonan I (RG I) and xylan epitopes in irx7 mucilage.
27333892	9	8	theme	mucilage	1281:1288	arg1	assays					1240:1245	whole-seed immunolabeling assays	1214:1245	whole-seed immunolabeling assays	1214:1245	Correspondingly, whole-seed immunolabeling assays and dot-immunoassays of extracted mucilage indicated dramatic changes in rhamnogalacturonan I (RG I) and xylan epitopes in irx7 mucilage.
27333892	5	9	theme	aberrant	604:611	arg1	morphology					613:622	no aberrant morphology	601:622	no aberrant morphology	601:622	The seed coat epidermis cells of irx7 displayed no aberrant morphology during differentiation, and these cells synthesized and deposited the same amount of mucilage as did wild type (WT) cells.
27333892	2	10	theme	plant	249:253	arg1	biosynthesis					265:276	plant cell wall biosynthesis	249:276	plant cell wall biosynthesis	249:276	This process is an ideal model system for the study of plant cell wall biosynthesis and modifications.
27333892	11	11	with	interaction	1693:1703	arg1	cellulose					1710:1718	cellulose	1710:1718	cellulose	1710:1718	Taken together, these results indicate that xylan synthesized by IRX7 plays an essential role in maintaining the adhesive property of seed coat mucilage, and its structural role is potentially implemented through its interaction with cellulose.
27333892	2	12	theme	modifications	282:294	arg1	study					240:244	the study	236:244	the study of plant cell wall biosynthesis and modifications	236:294	This process is an ideal model system for the study of plant cell wall biosynthesis and modifications.
27333892	3	13	from	mutation	325:332	arg1	Xylem					347:351	Irregular Xylem 7	337:353	Irregular Xylem 7 (IRX7)	337:360	In this study, we show that mutation in Irregular Xylem 7 (IRX7) results in a defect in mucilage adherence due to reduced xylan biosynthesis.
27333892	3	13	from	mutation	325:332	arg1	IRX7					356:359	IRX7	356:359	IRX7	356:359	In this study, we show that mutation in Irregular Xylem 7 (IRX7) results in a defect in mucilage adherence due to reduced xylan biosynthesis.
27333892	7	14	theme	xylan	942:946	arg1	linkages					930:937	glycosyl linkages	921:937	glycosyl linkages of xylan	921:946	Both the amount of xylose and the extent of glycosyl linkages of xylan was dramatically decreased in irx7 water-soluble and adherent mucilage compared to the WT.
27333892	9	15	theme	RG	1342:1343	arg1	I					1339:1339	rhamnogalacturonan I	1320:1339	rhamnogalacturonan I (RG I)	1320:1346	Correspondingly, whole-seed immunolabeling assays and dot-immunoassays of extracted mucilage indicated dramatic changes in rhamnogalacturonan I (RG I) and xylan epitopes in irx7 mucilage.
27333892	9	15	theme	RG	1342:1343	arg1	I					1345:1345	RG I	1342:1345	RG I	1342:1345	Correspondingly, whole-seed immunolabeling assays and dot-immunoassays of extracted mucilage indicated dramatic changes in rhamnogalacturonan I (RG I) and xylan epitopes in irx7 mucilage.
27333892	8	16	theme	polymeric	1043:1051	arg1	structure					1053:1061	The polymeric structure	1039:1061	The polymeric structure of water-soluble mucilage	1039:1087	The polymeric structure of water-soluble mucilage was altered in irx7, with a total loss of the higher molecular weight polymer components present in the WT.
27333892	1	17	theme	coat	139:142	arg1	cells					154:158	seed coat epidermis cells	134:158	seed coat epidermis cells	134:158	Large quantities of mucilage are synthesized in seed coat epidermis cells during seed coat differentiation.
27333892	9	18	theme	dramatic	1300:1307	arg1	changes					1309:1315	dramatic changes	1300:1315	dramatic changes in rhamnogalacturonan I (RG I) and xylan epitopes in irx7 mucilage	1300:1382	Correspondingly, whole-seed immunolabeling assays and dot-immunoassays of extracted mucilage indicated dramatic changes in rhamnogalacturonan I (RG I) and xylan epitopes in irx7 mucilage.
27333892	8	19	theme	water-soluble	1066:1078	arg1	mucilage					1080:1087	water-soluble mucilage	1066:1087	water-soluble mucilage	1066:1087	The polymeric structure of water-soluble mucilage was altered in irx7, with a total loss of the higher molecular weight polymer components present in the WT.
27333892	7	20	theme	glycosyl	921:928	arg1	linkages					930:937	glycosyl linkages	921:937	glycosyl linkages of xylan	921:946	Both the amount of xylose and the extent of glycosyl linkages of xylan was dramatically decreased in irx7 water-soluble and adherent mucilage compared to the WT.
27333892	0	21	theme	Irregular	0:8	arg1	xylem					10:14	Irregular xylem 7	0:16	Irregular xylem 7 (IRX7)	0:23	Irregular xylem 7 (IRX7) is required for anchoring seed coat mucilage in Arabidopsis.
27333892	0	21	theme	Irregular	0:8	arg1	IRX7					19:22	IRX7	19:22	IRX7	19:22	Irregular xylem 7 (IRX7) is required for anchoring seed coat mucilage in Arabidopsis.
27333892	3	22	theme	reduced	411:417	arg1	biosynthesis					425:436	reduced xylan biosynthesis	411:436	reduced xylan biosynthesis	411:436	In this study, we show that mutation in Irregular Xylem 7 (IRX7) results in a defect in mucilage adherence due to reduced xylan biosynthesis.
27333892	1	23	theme	epidermis	144:152	arg1	cells					154:158	seed coat epidermis cells	134:158	seed coat epidermis cells	134:158	Large quantities of mucilage are synthesized in seed coat epidermis cells during seed coat differentiation.
27333892	5	24	theme	same	694:697	arg1	amount					699:704	the same amount	690:704	the same amount of mucilage	690:716	The seed coat epidermis cells of irx7 displayed no aberrant morphology during differentiation, and these cells synthesized and deposited the same amount of mucilage as did wild type (WT) cells.
27333892	5	24	theme	same	694:697	arg1	mucilage					709:716	mucilage	709:716	mucilage	709:716	The seed coat epidermis cells of irx7 displayed no aberrant morphology during differentiation, and these cells synthesized and deposited the same amount of mucilage as did wild type (WT) cells.
27333892	7	25	theme	linkages	930:937	arg1	xylose					896:901	xylose	896:901	xylose	896:901	Both the amount of xylose and the extent of glycosyl linkages of xylan was dramatically decreased in irx7 water-soluble and adherent mucilage compared to the WT.
27333892	7	25	theme	linkages	930:937	arg1	extent					911:916	the extent	907:916	the extent of glycosyl linkages of xylan	907:946	Both the amount of xylose and the extent of glycosyl linkages of xylan was dramatically decreased in irx7 water-soluble and adherent mucilage compared to the WT.
27333892	7	25	theme	linkages	930:937	arg1	amount					886:891	the amount	882:891	the amount of xylose	882:901	Both the amount of xylose and the extent of glycosyl linkages of xylan was dramatically decreased in irx7 water-soluble and adherent mucilage compared to the WT.
27333892	7	25	theme	linkages	930:937	arg1	linkages					930:937	glycosyl linkages	921:937	glycosyl linkages of xylan	921:946	Both the amount of xylose and the extent of glycosyl linkages of xylan was dramatically decreased in irx7 water-soluble and adherent mucilage compared to the WT.
27333892	9	26	theme	rhamnogalacturonan	1320:1337	arg1	I					1339:1339	rhamnogalacturonan I	1320:1339	rhamnogalacturonan I (RG I)	1320:1346	Correspondingly, whole-seed immunolabeling assays and dot-immunoassays of extracted mucilage indicated dramatic changes in rhamnogalacturonan I (RG I) and xylan epitopes in irx7 mucilage.
27333892	9	26	theme	rhamnogalacturonan	1320:1337	arg1	I					1345:1345	RG I	1342:1345	RG I	1342:1345	Correspondingly, whole-seed immunolabeling assays and dot-immunoassays of extracted mucilage indicated dramatic changes in rhamnogalacturonan I (RG I) and xylan epitopes in irx7 mucilage.
27333892	3	27	theme	Irregular	337:345	arg1	Xylem					347:351	Irregular Xylem 7	337:353	Irregular Xylem 7 (IRX7)	337:360	In this study, we show that mutation in Irregular Xylem 7 (IRX7) results in a defect in mucilage adherence due to reduced xylan biosynthesis.
27333892	3	27	theme	Irregular	337:345	arg1	IRX7					356:359	IRX7	356:359	IRX7	356:359	In this study, we show that mutation in Irregular Xylem 7 (IRX7) results in a defect in mucilage adherence due to reduced xylan biosynthesis.
27333892	4	28	theme	4 days	476:481	arg1	DPA					498:500	DPA	498:500	DPA	498:500	IRX7 was expressed in the seeds from 4 days post-anthesis (DPA) to 13 DPA, with the peak of expression at 13 DPA.
27333892	4	28	theme	4 days	476:481	arg1	post-anthesis					483:495	4 days post-anthesis	476:495	4 days post-anthesis (DPA) to 13 DPA	476:511	IRX7 was expressed in the seeds from 4 days post-anthesis (DPA) to 13 DPA, with the peak of expression at 13 DPA.
27333892	9	29	theme	whole-seed	1214:1223	arg1	assays					1240:1245	whole-seed immunolabeling assays	1214:1245	whole-seed immunolabeling assays	1214:1245	Correspondingly, whole-seed immunolabeling assays and dot-immunoassays of extracted mucilage indicated dramatic changes in rhamnogalacturonan I (RG I) and xylan epitopes in irx7 mucilage.
27333892	2	30	theme	biosynthesis	265:276	arg1	study					240:244	the study	236:244	the study of plant cell wall biosynthesis and modifications	236:294	This process is an ideal model system for the study of plant cell wall biosynthesis and modifications.
27333892	10	31	theme	irx7	1461:1464	arg1	mucilage					1466:1473	irx7 mucilage	1461:1473	irx7 mucilage	1461:1473	Furthermore, the crystalline cellulose content was significantly reduced in irx7 mucilage.
27333892	4	32	from	post-anthesis	483:495	arg1	seeds					465:469	the seeds	461:469	the seeds from 4 days post-anthesis (DPA) to 13 DPA	461:511	IRX7 was expressed in the seeds from 4 days post-anthesis (DPA) to 13 DPA, with the peak of expression at 13 DPA.
27333892	8	33	theme	higher	1135:1140	arg1	components					1167:1176	the higher molecular weight polymer components	1131:1176	the higher molecular weight polymer components present in the WT	1131:1194	The polymeric structure of water-soluble mucilage was altered in irx7, with a total loss of the higher molecular weight polymer components present in the WT.
27333892	2	34	theme	wall	260:263	arg1	biosynthesis					265:276	plant cell wall biosynthesis	249:276	plant cell wall biosynthesis	249:276	This process is an ideal model system for the study of plant cell wall biosynthesis and modifications.
27333892	1	35	theme	seed	167:170	arg1	differentiation					177:191	seed coat differentiation	167:191	seed coat differentiation	167:191	Large quantities of mucilage are synthesized in seed coat epidermis cells during seed coat differentiation.
27333892	5	36	theme	mucilage	709:716	arg1	amount					699:704	the same amount	690:704	the same amount of mucilage	690:716	The seed coat epidermis cells of irx7 displayed no aberrant morphology during differentiation, and these cells synthesized and deposited the same amount of mucilage as did wild type (WT) cells.
27333892	5	36	theme	mucilage	709:716	arg1	mucilage					709:716	mucilage	709:716	mucilage	709:716	The seed coat epidermis cells of irx7 displayed no aberrant morphology during differentiation, and these cells synthesized and deposited the same amount of mucilage as did wild type (WT) cells.
27333892	9	37	theme	irx7	1370:1373	arg1	mucilage					1375:1382	irx7 mucilage	1370:1382	irx7 mucilage	1370:1382	Correspondingly, whole-seed immunolabeling assays and dot-immunoassays of extracted mucilage indicated dramatic changes in rhamnogalacturonan I (RG I) and xylan epitopes in irx7 mucilage.
27333892	5	38	theme	irx7	586:589	arg1	cells					577:581	The seed coat epidermis cells	553:581	The seed coat epidermis cells of irx7	553:589	The seed coat epidermis cells of irx7 displayed no aberrant morphology during differentiation, and these cells synthesized and deposited the same amount of mucilage as did wild type (WT) cells.
27333892	2	39	theme	cell	255:258	arg1	biosynthesis					265:276	plant cell wall biosynthesis	249:276	plant cell wall biosynthesis	249:276	This process is an ideal model system for the study of plant cell wall biosynthesis and modifications.
27333892	1	40	theme	coat	172:175	arg1	differentiation					177:191	seed coat differentiation	167:191	seed coat differentiation	167:191	Large quantities of mucilage are synthesized in seed coat epidermis cells during seed coat differentiation.
27333892	7	41	theme	adherent	1001:1008	arg1	mucilage					1010:1017	irx7 water-soluble and adherent mucilage	978:1017	irx7 water-soluble and adherent mucilage	978:1017	Both the amount of xylose and the extent of glycosyl linkages of xylan was dramatically decreased in irx7 water-soluble and adherent mucilage compared to the WT.
27333892	8	42	theme	components	1167:1176	arg1	loss					1123:1126	a total loss	1115:1126	a total loss of the higher molecular weight polymer components present in the WT	1115:1194	The polymeric structure of water-soluble mucilage was altered in irx7, with a total loss of the higher molecular weight polymer components present in the WT.
27333892	9	43	theme	xylan	1352:1356	arg1	epitopes					1358:1365	xylan epitopes	1352:1365	xylan epitopes	1352:1365	Correspondingly, whole-seed immunolabeling assays and dot-immunoassays of extracted mucilage indicated dramatic changes in rhamnogalacturonan I (RG I) and xylan epitopes in irx7 mucilage.
27333892	11	44	theme	structural	1638:1647	arg1	role					1649:1652	its structural role	1634:1652	its structural role	1634:1652	Taken together, these results indicate that xylan synthesized by IRX7 plays an essential role in maintaining the adhesive property of seed coat mucilage, and its structural role is potentially implemented through its interaction with cellulose.
27333892	5	45	theme	wild	725:728	arg1	WT					736:737	WT	736:737	WT	736:737	The seed coat epidermis cells of irx7 displayed no aberrant morphology during differentiation, and these cells synthesized and deposited the same amount of mucilage as did wild type (WT) cells.
27333892	5	45	theme	wild	725:728	arg1	type					730:733	wild type	725:733	wild type (WT) cells	725:744	The seed coat epidermis cells of irx7 displayed no aberrant morphology during differentiation, and these cells synthesized and deposited the same amount of mucilage as did wild type (WT) cells.
27333892	6	46	theme	layers	816:821	arg1	distribution					760:771	the distribution	756:771	the distribution of the water-soluble vs. adherent mucilage layers	756:821	However, the distribution of the water-soluble vs. adherent mucilage layers was significantly altered in irx7 compared to the WT.
27333892	9	47	theme	immunolabeling	1225:1238	arg1	assays					1240:1245	whole-seed immunolabeling assays	1214:1245	whole-seed immunolabeling assays	1214:1245	Correspondingly, whole-seed immunolabeling assays and dot-immunoassays of extracted mucilage indicated dramatic changes in rhamnogalacturonan I (RG I) and xylan epitopes in irx7 mucilage.
27333892	3	48	from	defect	375:380	arg1	adherence					394:402	mucilage adherence	385:402	mucilage adherence due to reduced xylan biosynthesis	385:436	In this study, we show that mutation in Irregular Xylem 7 (IRX7) results in a defect in mucilage adherence due to reduced xylan biosynthesis.
27333892	4	49	theme	expression	531:540	arg1	peak					523:526	the peak	519:526	the peak of expression at 13 DPA	519:550	IRX7 was expressed in the seeds from 4 days post-anthesis (DPA) to 13 DPA, with the peak of expression at 13 DPA.
27333892	7	50	theme	water-soluble	983:995	arg1	mucilage					1010:1017	irx7 water-soluble and adherent mucilage	978:1017	irx7 water-soluble and adherent mucilage	978:1017	Both the amount of xylose and the extent of glycosyl linkages of xylan was dramatically decreased in irx7 water-soluble and adherent mucilage compared to the WT.
27333892	6	51	theme	mucilage	807:814	arg1	layers					816:821	the water-soluble vs. adherent mucilage layers	776:821	the water-soluble vs. adherent mucilage layers	776:821	However, the distribution of the water-soluble vs. adherent mucilage layers was significantly altered in irx7 compared to the WT.
27333892	5	52	theme	seed	557:560	arg1	cells					577:581	The seed coat epidermis cells	553:581	The seed coat epidermis cells of irx7	553:589	The seed coat epidermis cells of irx7 displayed no aberrant morphology during differentiation, and these cells synthesized and deposited the same amount of mucilage as did wild type (WT) cells.
27333892	11	53	theme	essential	1555:1563	arg1	role					1565:1568	an essential role	1552:1568	an essential role	1552:1568	Taken together, these results indicate that xylan synthesized by IRX7 plays an essential role in maintaining the adhesive property of seed coat mucilage, and its structural role is potentially implemented through its interaction with cellulose.
27333892	1	54	theme	Large	86:90	arg1	quantities					92:101	Large quantities	86:101	Large quantities of mucilage	86:113	Large quantities of mucilage are synthesized in seed coat epidermis cells during seed coat differentiation.
27333892	8	55	theme	mucilage	1080:1087	arg1	structure					1053:1061	The polymeric structure	1039:1061	The polymeric structure of water-soluble mucilage	1039:1087	The polymeric structure of water-soluble mucilage was altered in irx7, with a total loss of the higher molecular weight polymer components present in the WT.
27333892	6	56	theme	adherent	798:805	arg1	layers					816:821	the water-soluble vs. adherent mucilage layers	776:821	the water-soluble vs. adherent mucilage layers	776:821	However, the distribution of the water-soluble vs. adherent mucilage layers was significantly altered in irx7 compared to the WT.
27333892	5	57	theme	coat	562:565	arg1	cells					577:581	The seed coat epidermis cells	553:581	The seed coat epidermis cells of irx7	553:589	The seed coat epidermis cells of irx7 displayed no aberrant morphology during differentiation, and these cells synthesized and deposited the same amount of mucilage as did wild type (WT) cells.
27333892	11	58	theme	adhesive	1589:1596	arg1	property					1598:1605	the adhesive property	1585:1605	the adhesive property of seed coat mucilage	1585:1627	Taken together, these results indicate that xylan synthesized by IRX7 plays an essential role in maintaining the adhesive property of seed coat mucilage, and its structural role is potentially implemented through its interaction with cellulose.
27333892	3	59	theme	xylan	419:423	arg1	biosynthesis					425:436	reduced xylan biosynthesis	411:436	reduced xylan biosynthesis	411:436	In this study, we show that mutation in Irregular Xylem 7 (IRX7) results in a defect in mucilage adherence due to reduced xylan biosynthesis.
27333892	5	60	dep	did	721:723	arg1	as					718:719	as	718:719	as	718:719	The seed coat epidermis cells of irx7 displayed no aberrant morphology during differentiation, and these cells synthesized and deposited the same amount of mucilage as did wild type (WT) cells.
27333892	5	61	theme	epidermis	567:575	arg1	cells					577:581	The seed coat epidermis cells	553:581	The seed coat epidermis cells of irx7	553:589	The seed coat epidermis cells of irx7 displayed no aberrant morphology during differentiation, and these cells synthesized and deposited the same amount of mucilage as did wild type (WT) cells.
27333892	8	62	from	present	1178:1184	arg1	WT					1193:1194	the WT	1189:1194	the WT	1189:1194	The polymeric structure of water-soluble mucilage was altered in irx7, with a total loss of the higher molecular weight polymer components present in the WT.
27333892	8	63	theme	molecular	1142:1150	arg1	components					1167:1176	the higher molecular weight polymer components	1131:1176	the higher molecular weight polymer components present in the WT	1131:1194	The polymeric structure of water-soluble mucilage was altered in irx7, with a total loss of the higher molecular weight polymer components present in the WT.
27333892	8	64	from	WT	1193:1194	arg1	present					1178:1184	present	1178:1184	present	1178:1184	The polymeric structure of water-soluble mucilage was altered in irx7, with a total loss of the higher molecular weight polymer components present in the WT.
27333892	8	65	theme	total	1117:1121	arg1	loss					1123:1126	a total loss	1115:1126	a total loss of the higher molecular weight polymer components present in the WT	1115:1194	The polymeric structure of water-soluble mucilage was altered in irx7, with a total loss of the higher molecular weight polymer components present in the WT.
27333892	8	66	attach	present	1178:1184	arg1	WT					1193:1194	the WT	1189:1194	the WT	1189:1194	The polymeric structure of water-soluble mucilage was altered in irx7, with a total loss of the higher molecular weight polymer components present in the WT.
27333892	8	66	attach	present	1178:1184	arg2	components					1167:1176	the higher molecular weight polymer components	1131:1176	the higher molecular weight polymer components present in the WT	1131:1194	The polymeric structure of water-soluble mucilage was altered in irx7, with a total loss of the higher molecular weight polymer components present in the WT.
27333892	1	67	theme	mucilage	106:113	arg1	quantities					92:101	Large quantities	86:101	Large quantities of mucilage	86:113	Large quantities of mucilage are synthesized in seed coat epidermis cells during seed coat differentiation.
27333892	4	68	from	DPA	548:550	arg1	peak					523:526	the peak	519:526	the peak of expression at 13 DPA	519:550	IRX7 was expressed in the seeds from 4 days post-anthesis (DPA) to 13 DPA, with the peak of expression at 13 DPA.
27333892	10	69	theme	cellulose	1414:1422	arg1	content					1424:1430	the crystalline cellulose content	1398:1430	the crystalline cellulose content	1398:1430	Furthermore, the crystalline cellulose content was significantly reduced in irx7 mucilage.
27333892	5	70	theme	type	730:733	arg1	cells					740:744	wild type (WT) cells	725:744	wild type (WT) cells	725:744	The seed coat epidermis cells of irx7 displayed no aberrant morphology during differentiation, and these cells synthesized and deposited the same amount of mucilage as did wild type (WT) cells.
27333892	2	71	theme	model	219:223	arg1	system					225:230	an ideal model system	210:230	an ideal model system for the study of plant cell wall biosynthesis and modifications	210:294	This process is an ideal model system for the study of plant cell wall biosynthesis and modifications.
27333892	2	71	theme	model	219:223	arg1	process					199:205	This process	194:205	This process	194:205	This process is an ideal model system for the study of plant cell wall biosynthesis and modifications.
27333892	3	72	theme	mucilage	385:392	arg1	adherence					394:402	mucilage adherence	385:402	mucilage adherence due to reduced xylan biosynthesis	385:436	In this study, we show that mutation in Irregular Xylem 7 (IRX7) results in a defect in mucilage adherence due to reduced xylan biosynthesis.
27333892	8	73	theme	polymer	1159:1165	arg1	components					1167:1176	the higher molecular weight polymer components	1131:1176	the higher molecular weight polymer components present in the WT	1131:1194	The polymeric structure of water-soluble mucilage was altered in irx7, with a total loss of the higher molecular weight polymer components present in the WT.
27333892	11	74	theme	coat	1615:1618	arg1	mucilage					1620:1627	seed coat mucilage	1610:1627	seed coat mucilage	1610:1627	Taken together, these results indicate that xylan synthesized by IRX7 plays an essential role in maintaining the adhesive property of seed coat mucilage, and its structural role is potentially implemented through its interaction with cellulose.
27333892	10	75	theme	crystalline	1402:1412	arg1	content					1424:1430	the crystalline cellulose content	1398:1430	the crystalline cellulose content	1398:1430	Furthermore, the crystalline cellulose content was significantly reduced in irx7 mucilage.
27333892	6	76	theme	water-soluble	780:792	arg1	layers					816:821	the water-soluble vs. adherent mucilage layers	776:821	the water-soluble vs. adherent mucilage layers	776:821	However, the distribution of the water-soluble vs. adherent mucilage layers was significantly altered in irx7 compared to the WT.
27333892	2	77	theme	ideal	213:217	arg1	system					225:230	an ideal model system	210:230	an ideal model system for the study of plant cell wall biosynthesis and modifications	210:294	This process is an ideal model system for the study of plant cell wall biosynthesis and modifications.
27333892	2	77	theme	ideal	213:217	arg1	process					199:205	This process	194:205	This process	194:205	This process is an ideal model system for the study of plant cell wall biosynthesis and modifications.
27333892	9	78	from	changes	1309:1315	arg1	epitopes					1358:1365	xylan epitopes	1352:1365	xylan epitopes	1352:1365	Correspondingly, whole-seed immunolabeling assays and dot-immunoassays of extracted mucilage indicated dramatic changes in rhamnogalacturonan I (RG I) and xylan epitopes in irx7 mucilage.
27333892	9	78	from	changes	1309:1315	arg1	mucilage					1375:1382	irx7 mucilage	1370:1382	irx7 mucilage	1370:1382	Correspondingly, whole-seed immunolabeling assays and dot-immunoassays of extracted mucilage indicated dramatic changes in rhamnogalacturonan I (RG I) and xylan epitopes in irx7 mucilage.
27333892	9	78	from	changes	1309:1315	arg1	I					1339:1339	rhamnogalacturonan I	1320:1339	rhamnogalacturonan I (RG I)	1320:1346	Correspondingly, whole-seed immunolabeling assays and dot-immunoassays of extracted mucilage indicated dramatic changes in rhamnogalacturonan I (RG I) and xylan epitopes in irx7 mucilage.
27333892	9	78	from	changes	1309:1315	arg1	I					1345:1345	RG I	1342:1345	RG I	1342:1345	Correspondingly, whole-seed immunolabeling assays and dot-immunoassays of extracted mucilage indicated dramatic changes in rhamnogalacturonan I (RG I) and xylan epitopes in irx7 mucilage.
27333892	8	79	theme	weight	1152:1157	arg1	components					1167:1176	the higher molecular weight polymer components	1131:1176	the higher molecular weight polymer components present in the WT	1131:1194	The polymeric structure of water-soluble mucilage was altered in irx7, with a total loss of the higher molecular weight polymer components present in the WT.
27333892	11	80	theme	seed	1610:1613	arg1	mucilage					1620:1627	seed coat mucilage	1610:1627	seed coat mucilage	1610:1627	Taken together, these results indicate that xylan synthesized by IRX7 plays an essential role in maintaining the adhesive property of seed coat mucilage, and its structural role is potentially implemented through its interaction with cellulose.
27987913	0	0	theme	sepiolite	73:81	arg1	clay					91:94	sepiolite fibrous clay	73:94	sepiolite fibrous clay	73:94	Bionanocomposite foams based on the assembly of starch and alginate with sepiolite fibrous clay.
27987913	2	1	from	groups	449:454	arg1	chains					478:483	the polysaccharide chains	459:483	the polysaccharide chains	459:483	Spectroscopic techniques were applied in order to assess the interaction mechanism established between the inorganic fibers and the polysaccharide chains, which is established between the hydroxyl groups in the polysaccharide chains and the silanol groups at the external surface of the sepiolite fibers.
27987913	5	2	theme	role	1035:1038	arg1	evaluation					1017:1026	a preliminary evaluation	1003:1026	a preliminary evaluation of the role of the inorganic fibers	1003:1062	Horizontal burning tests were carried out for a preliminary evaluation of the role of the inorganic fibers on the fire resistance properties of the bionanocomposite foams, revealing that bionanocomposite foams with sepiolite content >25% behave as auto-extinguishable materials.
27987913	5	3	theme	%	1193:1193	arg1	content					1182:1188	sepiolite content	1172:1188	sepiolite content >25%	1172:1193	Horizontal burning tests were carried out for a preliminary evaluation of the role of the inorganic fibers on the fire resistance properties of the bionanocomposite foams, revealing that bionanocomposite foams with sepiolite content >25% behave as auto-extinguishable materials.
27987913	5	4	theme	bionanocomposite	1105:1120	arg1	foams					1122:1126	the bionanocomposite foams	1101:1126	the bionanocomposite foams	1101:1126	Horizontal burning tests were carried out for a preliminary evaluation of the role of the inorganic fibers on the fire resistance properties of the bionanocomposite foams, revealing that bionanocomposite foams with sepiolite content >25% behave as auto-extinguishable materials.
27987913	4	5	from	29MPa	887:891	arg1	foams					896:900	foams	896:900	foams containing 10% starch, 40% sepiolite and 50% alginate	896:954	Mechanical properties were also determined for the studied foams, showing an increase in compression moduli from 7.3MPa in the foam without sepiolite to 29MPa in foams containing 10% starch, 40% sepiolite and 50% alginate.
27987913	2	6	theme	sepiolite	539:547	arg1	fibers					549:554	the sepiolite fibers	535:554	the sepiolite fibers	535:554	Spectroscopic techniques were applied in order to assess the interaction mechanism established between the inorganic fibers and the polysaccharide chains, which is established between the hydroxyl groups in the polysaccharide chains and the silanol groups at the external surface of the sepiolite fibers.
27987913	3	7	theme	sepiolite	705:713	arg1	content					715:721	the sepiolite content	701:721	the sepiolite content	701:721	The textural properties studied by means of mercury intrusion porosimetry, FE-SEM and X-ray microtomography, revealed a decrease in porosity as the sepiolite content increased.
27987913	5	8	with	foams	1161:1165	arg1	content					1182:1188	sepiolite content	1172:1188	sepiolite content >25%	1172:1193	Horizontal burning tests were carried out for a preliminary evaluation of the role of the inorganic fibers on the fire resistance properties of the bionanocomposite foams, revealing that bionanocomposite foams with sepiolite content >25% behave as auto-extinguishable materials.
27987913	0	9	theme	fibrous	83:89	arg1	clay					91:94	sepiolite fibrous clay	73:94	sepiolite fibrous clay	73:94	Bionanocomposite foams based on the assembly of starch and alginate with sepiolite fibrous clay.
27987913	5	10	theme	fire	1071:1074	arg1	properties					1087:1096	the fire resistance properties	1067:1096	the fire resistance properties of the bionanocomposite foams	1067:1126	Horizontal burning tests were carried out for a preliminary evaluation of the role of the inorganic fibers on the fire resistance properties of the bionanocomposite foams, revealing that bionanocomposite foams with sepiolite content >25% behave as auto-extinguishable materials.
27987913	4	11	theme	studied	785:791	arg1	foams					793:797	the studied foams	781:797	the studied foams	781:797	Mechanical properties were also determined for the studied foams, showing an increase in compression moduli from 7.3MPa in the foam without sepiolite to 29MPa in foams containing 10% starch, 40% sepiolite and 50% alginate.
27987913	3	12	theme	textural	561:568	arg1	FE-SEM					632:637	FE-SEM	632:637	FE-SEM	632:637	The textural properties studied by means of mercury intrusion porosimetry, FE-SEM and X-ray microtomography, revealed a decrease in porosity as the sepiolite content increased.
27987913	3	12	theme	textural	561:568	arg1	microtomography					649:663	X-ray microtomography	643:663	X-ray microtomography	643:663	The textural properties studied by means of mercury intrusion porosimetry, FE-SEM and X-ray microtomography, revealed a decrease in porosity as the sepiolite content increased.
27987913	3	12	theme	textural	561:568	arg1	properties					570:579	The textural properties	557:579	The textural properties studied by means of mercury intrusion porosimetry	557:629	The textural properties studied by means of mercury intrusion porosimetry, FE-SEM and X-ray microtomography, revealed a decrease in porosity as the sepiolite content increased.
27987913	5	13	theme	auto-extinguishable	1205:1223	arg1	materials					1225:1233	auto-extinguishable materials	1205:1233	auto-extinguishable materials	1205:1233	Horizontal burning tests were carried out for a preliminary evaluation of the role of the inorganic fibers on the fire resistance properties of the bionanocomposite foams, revealing that bionanocomposite foams with sepiolite content >25% behave as auto-extinguishable materials.
27987913	6	14	theme	compression	1348:1358	arg1	modulus					1360:1366	the compression modulus	1344:1366	the compression modulus up to 40MPa for the optimal composition	1344:1406	Post-synthesis cross-linking with CaCl2 was carried out in some of these samples, leading to an increase in the compression modulus up to 40MPa for the optimal composition.
27987913	4	15	theme	Mechanical	734:743	arg1	properties					745:754	Mechanical properties	734:754	Mechanical properties	734:754	Mechanical properties were also determined for the studied foams, showing an increase in compression moduli from 7.3MPa in the foam without sepiolite to 29MPa in foams containing 10% starch, 40% sepiolite and 50% alginate.
27987913	6	16	dep	40MPa	1374:1378	arg1	up					1368:1369	up	1368:1369	up	1368:1369	Post-synthesis cross-linking with CaCl2 was carried out in some of these samples, leading to an increase in the compression modulus up to 40MPa for the optimal composition.
27987913	5	17	theme	burning	968:974	arg1	tests					976:980	Horizontal burning tests	957:980	Horizontal burning tests	957:980	Horizontal burning tests were carried out for a preliminary evaluation of the role of the inorganic fibers on the fire resistance properties of the bionanocomposite foams, revealing that bionanocomposite foams with sepiolite content >25% behave as auto-extinguishable materials.
27987913	4	18	theme	%	945:945	arg1	alginate					947:954	50% alginate	943:954	50% alginate	943:954	Mechanical properties were also determined for the studied foams, showing an increase in compression moduli from 7.3MPa in the foam without sepiolite to 29MPa in foams containing 10% starch, 40% sepiolite and 50% alginate.
27987913	4	19	theme	compression	823:833	arg1	moduli					835:840	compression moduli	823:840	compression moduli from 7.3MPa	823:852	Mechanical properties were also determined for the studied foams, showing an increase in compression moduli from 7.3MPa in the foam without sepiolite to 29MPa in foams containing 10% starch, 40% sepiolite and 50% alginate.
27987913	4	20	contain	containing	902:911	arg2	sepiolite					929:937	40% sepiolite	925:937	40% sepiolite	925:937	Mechanical properties were also determined for the studied foams, showing an increase in compression moduli from 7.3MPa in the foam without sepiolite to 29MPa in foams containing 10% starch, 40% sepiolite and 50% alginate.
27987913	4	20	contain	containing	902:911	arg2	starch					917:922	starch	917:922	starch	917:922	Mechanical properties were also determined for the studied foams, showing an increase in compression moduli from 7.3MPa in the foam without sepiolite to 29MPa in foams containing 10% starch, 40% sepiolite and 50% alginate.
27987913	4	20	contain	containing	902:911	arg2	alginate					947:954	50% alginate	943:954	50% alginate	943:954	Mechanical properties were also determined for the studied foams, showing an increase in compression moduli from 7.3MPa in the foam without sepiolite to 29MPa in foams containing 10% starch, 40% sepiolite and 50% alginate.
27987913	4	20	contain	containing	902:911	arg1	foams					896:900	foams	896:900	foams containing 10% starch, 40% sepiolite and 50% alginate	896:954	Mechanical properties were also determined for the studied foams, showing an increase in compression moduli from 7.3MPa in the foam without sepiolite to 29MPa in foams containing 10% starch, 40% sepiolite and 50% alginate.
27987913	1	21	theme	microfibrous	161:172	arg1	sepiolite					187:195	the microfibrous clay mineral sepiolite	157:195	the microfibrous clay mineral sepiolite	157:195	Bionanocomposite foams based on alginate, potato starch and the microfibrous clay mineral sepiolite as reinforcing filler were prepared by lyophilization.
27987913	2	22	theme	polysaccharide	463:476	arg1	chains					478:483	the polysaccharide chains	459:483	the polysaccharide chains	459:483	Spectroscopic techniques were applied in order to assess the interaction mechanism established between the inorganic fibers and the polysaccharide chains, which is established between the hydroxyl groups in the polysaccharide chains and the silanol groups at the external surface of the sepiolite fibers.
27987913	6	23	from	increase	1332:1339	arg1	modulus					1360:1366	the compression modulus	1344:1366	the compression modulus up to 40MPa for the optimal composition	1344:1406	Post-synthesis cross-linking with CaCl2 was carried out in some of these samples, leading to an increase in the compression modulus up to 40MPa for the optimal composition.
27987913	0	24	theme	alginate	59:66	arg1	assembly					36:43	the assembly	32:43	the assembly of starch and alginate with sepiolite fibrous clay	32:94	Bionanocomposite foams based on the assembly of starch and alginate with sepiolite fibrous clay.
27987913	5	25	theme	resistance	1076:1085	arg1	properties					1087:1096	the fire resistance properties	1067:1096	the fire resistance properties of the bionanocomposite foams	1067:1126	Horizontal burning tests were carried out for a preliminary evaluation of the role of the inorganic fibers on the fire resistance properties of the bionanocomposite foams, revealing that bionanocomposite foams with sepiolite content >25% behave as auto-extinguishable materials.
27987913	1	26	theme	clay	174:177	arg1	sepiolite					187:195	the microfibrous clay mineral sepiolite	157:195	the microfibrous clay mineral sepiolite	157:195	Bionanocomposite foams based on alginate, potato starch and the microfibrous clay mineral sepiolite as reinforcing filler were prepared by lyophilization.
27987913	5	27	theme	foams	1122:1126	arg1	properties					1087:1096	the fire resistance properties	1067:1096	the fire resistance properties of the bionanocomposite foams	1067:1126	Horizontal burning tests were carried out for a preliminary evaluation of the role of the inorganic fibers on the fire resistance properties of the bionanocomposite foams, revealing that bionanocomposite foams with sepiolite content >25% behave as auto-extinguishable materials.
27987913	2	28	theme	interaction	313:323	arg1	mechanism					325:333	the interaction mechanism	309:333	the interaction mechanism established between the inorganic fibers and the polysaccharide chains, which is established between the hydroxyl groups in the polysaccharide chains and the silanol groups at the external surface of the sepiolite fibers	309:554	Spectroscopic techniques were applied in order to assess the interaction mechanism established between the inorganic fibers and the polysaccharide chains, which is established between the hydroxyl groups in the polysaccharide chains and the silanol groups at the external surface of the sepiolite fibers.
27987913	1	29	theme	mineral	179:185	arg1	sepiolite					187:195	the microfibrous clay mineral sepiolite	157:195	the microfibrous clay mineral sepiolite	157:195	Bionanocomposite foams based on alginate, potato starch and the microfibrous clay mineral sepiolite as reinforcing filler were prepared by lyophilization.
27987913	0	30	dep	foams	17:21	arg1	based					23:27	based	23:27	foams based on the assembly of starch and alginate with sepiolite fibrous clay	17:94	Bionanocomposite foams based on the assembly of starch and alginate with sepiolite fibrous clay.
27987913	4	31	theme	40	925:926	arg1	%					927:927	%	927:927	%	927:927	Mechanical properties were also determined for the studied foams, showing an increase in compression moduli from 7.3MPa in the foam without sepiolite to 29MPa in foams containing 10% starch, 40% sepiolite and 50% alginate.
27987913	2	32	theme	hydroxyl	440:447	arg1	groups					449:454	the hydroxyl groups	436:454	the hydroxyl groups in the polysaccharide chains	436:483	Spectroscopic techniques were applied in order to assess the interaction mechanism established between the inorganic fibers and the polysaccharide chains, which is established between the hydroxyl groups in the polysaccharide chains and the silanol groups at the external surface of the sepiolite fibers.
27987913	6	33	theme	optimal	1388:1394	arg1	composition					1396:1406	the optimal composition	1384:1406	the optimal composition	1384:1406	Post-synthesis cross-linking with CaCl2 was carried out in some of these samples, leading to an increase in the compression modulus up to 40MPa for the optimal composition.
27987913	5	34	theme	inorganic	1047:1055	arg1	fibers					1057:1062	the inorganic fibers	1043:1062	the inorganic fibers	1043:1062	Horizontal burning tests were carried out for a preliminary evaluation of the role of the inorganic fibers on the fire resistance properties of the bionanocomposite foams, revealing that bionanocomposite foams with sepiolite content >25% behave as auto-extinguishable materials.
27987913	1	35	theme	Bionanocomposite	97:112	arg1	foams					114:118	Bionanocomposite foams	97:118	Bionanocomposite foams based on alginate, potato starch and the microfibrous clay mineral sepiolite as reinforcing filler	97:217	Bionanocomposite foams based on alginate, potato starch and the microfibrous clay mineral sepiolite as reinforcing filler were prepared by lyophilization.
27987913	2	36	theme	silanol	493:499	arg1	groups					501:506	the silanol groups	489:506	the silanol groups at the external surface of the sepiolite fibers	489:554	Spectroscopic techniques were applied in order to assess the interaction mechanism established between the inorganic fibers and the polysaccharide chains, which is established between the hydroxyl groups in the polysaccharide chains and the silanol groups at the external surface of the sepiolite fibers.
27987913	5	37	theme	>25	1190:1192	arg1	%					1193:1193	%	1193:1193	%	1193:1193	Horizontal burning tests were carried out for a preliminary evaluation of the role of the inorganic fibers on the fire resistance properties of the bionanocomposite foams, revealing that bionanocomposite foams with sepiolite content >25% behave as auto-extinguishable materials.
27987913	2	38	from	groups	501:506	arg1	chains					478:483	the polysaccharide chains	459:483	the polysaccharide chains	459:483	Spectroscopic techniques were applied in order to assess the interaction mechanism established between the inorganic fibers and the polysaccharide chains, which is established between the hydroxyl groups in the polysaccharide chains and the silanol groups at the external surface of the sepiolite fibers.
27987913	3	39	theme	intrusion	609:617	arg1	porosimetry					619:629	mercury intrusion porosimetry	601:629	mercury intrusion porosimetry	601:629	The textural properties studied by means of mercury intrusion porosimetry, FE-SEM and X-ray microtomography, revealed a decrease in porosity as the sepiolite content increased.
27987913	1	40	theme	reinforcing	200:210	arg1	filler					212:217	reinforcing filler	200:217	reinforcing filler	200:217	Bionanocomposite foams based on alginate, potato starch and the microfibrous clay mineral sepiolite as reinforcing filler were prepared by lyophilization.
27987913	2	41	theme	fibers	549:554	arg1	surface					524:530	the external surface	511:530	the external surface of the sepiolite fibers	511:554	Spectroscopic techniques were applied in order to assess the interaction mechanism established between the inorganic fibers and the polysaccharide chains, which is established between the hydroxyl groups in the polysaccharide chains and the silanol groups at the external surface of the sepiolite fibers.
27987913	5	42	theme	preliminary	1005:1015	arg1	evaluation					1017:1026	a preliminary evaluation	1003:1026	a preliminary evaluation of the role of the inorganic fibers	1003:1062	Horizontal burning tests were carried out for a preliminary evaluation of the role of the inorganic fibers on the fire resistance properties of the bionanocomposite foams, revealing that bionanocomposite foams with sepiolite content >25% behave as auto-extinguishable materials.
27987913	0	43	theme	starch	48:53	arg1	assembly					36:43	the assembly	32:43	the assembly of starch and alginate with sepiolite fibrous clay	32:94	Bionanocomposite foams based on the assembly of starch and alginate with sepiolite fibrous clay.
27987913	4	44	theme	50	943:944	arg1	%					945:945	%	945:945	%	945:945	Mechanical properties were also determined for the studied foams, showing an increase in compression moduli from 7.3MPa in the foam without sepiolite to 29MPa in foams containing 10% starch, 40% sepiolite and 50% alginate.
27987913	4	45	theme	10	913:914	arg1	%					915:915	%	915:915	%	915:915	Mechanical properties were also determined for the studied foams, showing an increase in compression moduli from 7.3MPa in the foam without sepiolite to 29MPa in foams containing 10% starch, 40% sepiolite and 50% alginate.
27987913	5	46	theme	sepiolite	1172:1180	arg1	content					1182:1188	sepiolite content	1172:1188	sepiolite content >25%	1172:1193	Horizontal burning tests were carried out for a preliminary evaluation of the role of the inorganic fibers on the fire resistance properties of the bionanocomposite foams, revealing that bionanocomposite foams with sepiolite content >25% behave as auto-extinguishable materials.
27987913	5	47	theme	Horizontal	957:966	arg1	tests					976:980	Horizontal burning tests	957:980	Horizontal burning tests	957:980	Horizontal burning tests were carried out for a preliminary evaluation of the role of the inorganic fibers on the fire resistance properties of the bionanocomposite foams, revealing that bionanocomposite foams with sepiolite content >25% behave as auto-extinguishable materials.
27987913	4	48	from	7.3MPa	847:852	arg1	increase					811:818	an increase	808:818	an increase in compression moduli from 7.3MPa in the foam	808:864	Mechanical properties were also determined for the studied foams, showing an increase in compression moduli from 7.3MPa in the foam without sepiolite to 29MPa in foams containing 10% starch, 40% sepiolite and 50% alginate.
27987913	4	48	from	7.3MPa	847:852	arg1	moduli					835:840	compression moduli	823:840	compression moduli from 7.3MPa	823:852	Mechanical properties were also determined for the studied foams, showing an increase in compression moduli from 7.3MPa in the foam without sepiolite to 29MPa in foams containing 10% starch, 40% sepiolite and 50% alginate.
27987913	2	49	theme	inorganic	359:367	arg1	fibers					369:374	the inorganic fibers	355:374	the inorganic fibers	355:374	Spectroscopic techniques were applied in order to assess the interaction mechanism established between the inorganic fibers and the polysaccharide chains, which is established between the hydroxyl groups in the polysaccharide chains and the silanol groups at the external surface of the sepiolite fibers.
27987913	4	50	theme	%	927:927	arg1	sepiolite					929:937	40% sepiolite	925:937	40% sepiolite	925:937	Mechanical properties were also determined for the studied foams, showing an increase in compression moduli from 7.3MPa in the foam without sepiolite to 29MPa in foams containing 10% starch, 40% sepiolite and 50% alginate.
27987913	5	51	theme	bionanocomposite	1144:1159	arg1	foams					1161:1165	bionanocomposite foams	1144:1165	bionanocomposite foams with sepiolite content >25%	1144:1193	Horizontal burning tests were carried out for a preliminary evaluation of the role of the inorganic fibers on the fire resistance properties of the bionanocomposite foams, revealing that bionanocomposite foams with sepiolite content >25% behave as auto-extinguishable materials.
27987913	3	52	theme	mercury	601:607	arg1	porosimetry					619:629	mercury intrusion porosimetry	601:629	mercury intrusion porosimetry	601:629	The textural properties studied by means of mercury intrusion porosimetry, FE-SEM and X-ray microtomography, revealed a decrease in porosity as the sepiolite content increased.
27987913	2	53	from	surface	524:530	arg1	groups					449:454	the hydroxyl groups	436:454	the hydroxyl groups in the polysaccharide chains	436:483	Spectroscopic techniques were applied in order to assess the interaction mechanism established between the inorganic fibers and the polysaccharide chains, which is established between the hydroxyl groups in the polysaccharide chains and the silanol groups at the external surface of the sepiolite fibers.
27987913	2	53	from	surface	524:530	arg1	groups					501:506	the silanol groups	489:506	the silanol groups at the external surface of the sepiolite fibers	489:554	Spectroscopic techniques were applied in order to assess the interaction mechanism established between the inorganic fibers and the polysaccharide chains, which is established between the hydroxyl groups in the polysaccharide chains and the silanol groups at the external surface of the sepiolite fibers.
27987913	6	54	theme	Post-synthesis	1236:1249	arg1	cross-linking					1251:1263	Post-synthesis cross-linking	1236:1263	Post-synthesis cross-linking with CaCl2	1236:1274	Post-synthesis cross-linking with CaCl2 was carried out in some of these samples, leading to an increase in the compression modulus up to 40MPa for the optimal composition.
27987913	4	55	from	increase	811:818	arg1	7.3MPa					847:852	7.3MPa	847:852	7.3MPa	847:852	Mechanical properties were also determined for the studied foams, showing an increase in compression moduli from 7.3MPa in the foam without sepiolite to 29MPa in foams containing 10% starch, 40% sepiolite and 50% alginate.
27987913	4	55	from	increase	811:818	arg1	foam					861:864	the foam	857:864	the foam	857:864	Mechanical properties were also determined for the studied foams, showing an increase in compression moduli from 7.3MPa in the foam without sepiolite to 29MPa in foams containing 10% starch, 40% sepiolite and 50% alginate.
27987913	4	55	from	increase	811:818	arg1	moduli					835:840	compression moduli	823:840	compression moduli from 7.3MPa	823:852	Mechanical properties were also determined for the studied foams, showing an increase in compression moduli from 7.3MPa in the foam without sepiolite to 29MPa in foams containing 10% starch, 40% sepiolite and 50% alginate.
27987913	4	56	theme	%	915:915	arg1	starch					917:922	starch	917:922	starch	917:922	Mechanical properties were also determined for the studied foams, showing an increase in compression moduli from 7.3MPa in the foam without sepiolite to 29MPa in foams containing 10% starch, 40% sepiolite and 50% alginate.
27987913	3	57	theme	X-ray	643:647	arg1	microtomography					649:663	X-ray microtomography	643:663	X-ray microtomography	643:663	The textural properties studied by means of mercury intrusion porosimetry, FE-SEM and X-ray microtomography, revealed a decrease in porosity as the sepiolite content increased.
27987913	3	57	theme	X-ray	643:647	arg1	properties					570:579	The textural properties	557:579	The textural properties studied by means of mercury intrusion porosimetry	557:629	The textural properties studied by means of mercury intrusion porosimetry, FE-SEM and X-ray microtomography, revealed a decrease in porosity as the sepiolite content increased.
27987913	5	58	theme	fibers	1057:1062	arg1	role					1035:1038	the role	1031:1038	the role of the inorganic fibers	1031:1062	Horizontal burning tests were carried out for a preliminary evaluation of the role of the inorganic fibers on the fire resistance properties of the bionanocomposite foams, revealing that bionanocomposite foams with sepiolite content >25% behave as auto-extinguishable materials.
27987913	2	59	theme	external	515:522	arg1	surface					524:530	the external surface	511:530	the external surface of the sepiolite fibers	511:554	Spectroscopic techniques were applied in order to assess the interaction mechanism established between the inorganic fibers and the polysaccharide chains, which is established between the hydroxyl groups in the polysaccharide chains and the silanol groups at the external surface of the sepiolite fibers.
27987913	2	60	theme	Spectroscopic	252:264	arg1	techniques					266:275	Spectroscopic techniques	252:275	Spectroscopic techniques	252:275	Spectroscopic techniques were applied in order to assess the interaction mechanism established between the inorganic fibers and the polysaccharide chains, which is established between the hydroxyl groups in the polysaccharide chains and the silanol groups at the external surface of the sepiolite fibers.
27987913	2	61	theme	polysaccharide	384:397	arg1	chains					399:404	the polysaccharide chains	380:404	the polysaccharide chains	380:404	Spectroscopic techniques were applied in order to assess the interaction mechanism established between the inorganic fibers and the polysaccharide chains, which is established between the hydroxyl groups in the polysaccharide chains and the silanol groups at the external surface of the sepiolite fibers.
27987913	6	62	with	cross-linking	1251:1263	arg1	CaCl2					1270:1274	CaCl2	1270:1274	CaCl2	1270:1274	Post-synthesis cross-linking with CaCl2 was carried out in some of these samples, leading to an increase in the compression modulus up to 40MPa for the optimal composition.
27987913	3	63	from	decrease	677:684	arg1	porosity					689:696	porosity	689:696	porosity	689:696	The textural properties studied by means of mercury intrusion porosimetry, FE-SEM and X-ray microtomography, revealed a decrease in porosity as the sepiolite content increased.
27987913	0	64	with	assembly	36:43	arg1	clay					91:94	sepiolite fibrous clay	73:94	sepiolite fibrous clay	73:94	Bionanocomposite foams based on the assembly of starch and alginate with sepiolite fibrous clay.
27987913	1	65	theme	potato	139:144	arg1	starch					146:151	potato starch	139:151	potato starch	139:151	Bionanocomposite foams based on alginate, potato starch and the microfibrous clay mineral sepiolite as reinforcing filler were prepared by lyophilization.
28867105	8	0	theme	overall	1047:1053	arg1	cost					1055:1058	overall cost	1047:1058	overall cost of production	1047:1072	It is implied the raw material costs of two identified cultivars (CSH23 & CSH13R) would help the industry to reduce overall cost of production and offer a better profit margins over the varieties.
28867105	0	1	theme	composition	57:67	arg1	evaluation					9:18	evaluation	9:18	evaluation of starch, dietary fiber and mineral composition of cookies	9:78	Study on evaluation of starch, dietary fiber and mineral composition of cookies developed from 12 sorghum cultivars.
28867105	4	2	theme	P	539:539	arg1	content					590:596	The highest Mg (56.24±0.03mg/100g) P (255.54±0.03mg/100g), and K (124.26±0.02mg/100g) content	504:596	The highest Mg (56.24±0.03mg/100g) P (255.54±0.03mg/100g), and K (124.26±0.02mg/100g) content	504:596	The highest Mg (56.24±0.03mg/100g) P (255.54±0.03mg/100g), and K (124.26±0.02mg/100g) content were found in C43 cultivar.
28867105	6	3	from	fiber	820:824	arg1	rich					804:807	rich	804:807	rich	804:807	The sensory scores for overall acceptability of cookies were highest in CSH23, CSH13R and CSV18R cultivars which are rich in dietary fiber and minerals.
28867105	0	4	from	Study	0:4	arg1	evaluation					9:18	evaluation	9:18	evaluation of starch, dietary fiber and mineral composition of cookies	9:78	Study on evaluation of starch, dietary fiber and mineral composition of cookies developed from 12 sorghum cultivars.
28867105	8	5	theme	material	953:960	arg1	costs					962:966	the raw material costs	945:966	the raw material costs of two identified cultivars (CSH23 & CSH13R)	945:1011	It is implied the raw material costs of two identified cultivars (CSH23 & CSH13R) would help the industry to reduce overall cost of production and offer a better profit margins over the varieties.
28867105	2	6	theme	cookies	278:284	arg1	content					267:273	Protein and fat content	251:273	content	267:273	Protein and fat content of cookies were ranged from 5.89±0.04 to 8.27±0.21% and 21.03±0.01 to 23.08±0.03% respectively.
28867105	0	7	theme	cookies	72:78	arg1	composition					57:67	mineral composition	49:67	mineral composition	49:67	Study on evaluation of starch, dietary fiber and mineral composition of cookies developed from 12 sorghum cultivars.
28867105	0	7	theme	cookies	72:78	arg1	starch					23:28	starch	23:28	starch	23:28	Study on evaluation of starch, dietary fiber and mineral composition of cookies developed from 12 sorghum cultivars.
28867105	0	7	theme	cookies	72:78	arg1	fiber					39:43	dietary fiber	31:43	dietary fiber	31:43	Study on evaluation of starch, dietary fiber and mineral composition of cookies developed from 12 sorghum cultivars.
28867105	5	8	theme	iron	654:657	arg1	1.23±0.01mg/100g					668:683	1.23±0.01mg/100g	668:683	1.23±0.01mg/100g	668:683	CSV18R was reported highest iron content (1.23±0.01mg/100g).
28867105	5	8	theme	iron	654:657	arg1	content					659:665	highest iron content	646:665	highest iron content (1.23±0.01mg/100g)	646:684	CSV18R was reported highest iron content (1.23±0.01mg/100g).
28867105	8	9	theme	raw	949:951	arg1	costs					962:966	the raw material costs	945:966	the raw material costs of two identified cultivars (CSH23 & CSH13R)	945:1011	It is implied the raw material costs of two identified cultivars (CSH23 & CSH13R) would help the industry to reduce overall cost of production and offer a better profit margins over the varieties.
28867105	6	10	from	minerals	830:837	arg1	rich					804:807	rich	804:807	rich	804:807	The sensory scores for overall acceptability of cookies were highest in CSH23, CSH13R and CSV18R cultivars which are rich in dietary fiber and minerals.
28867105	8	11	theme	production	1063:1072	arg1	cost					1055:1058	overall cost	1047:1058	overall cost of production	1047:1072	It is implied the raw material costs of two identified cultivars (CSH23 & CSH13R) would help the industry to reduce overall cost of production and offer a better profit margins over the varieties.
28867105	7	12	theme	grain	887:891	arg1	lower					909:913	lower	909:913	lower	909:913	Normally the hybrids are high yielders and the grain price/qt is 20% lower than varieties.
28867105	7	12	theme	grain	887:891	arg1	price/qt					893:900	the grain price/qt	883:900	the grain price/qt	883:900	Normally the hybrids are high yielders and the grain price/qt is 20% lower than varieties.
28867105	3	13	dep	highest	473:479	arg1	%					500:500	9.27±0.01%	491:500	9.27±0.01%	491:500	The starch content of cookie ranged between 47.06±0.01 and 42.15±0.03% and dietary fiber was reported highest in CSH14 (9.27±0.01%).
28867105	1	14	theme	best	145:148	arg1	cultivars					150:158	best cultivars	145:158	best cultivars suitable for sorghum cookies accordingly nutrient	145:208	The study aimed to identify best cultivars suitable for sorghum cookies accordingly nutrient and mineral compositions were evaluated.
28867105	2	15	theme	fat	263:265	arg1	content					267:273	Protein and fat content	251:273	content	267:273	Protein and fat content of cookies were ranged from 5.89±0.04 to 8.27±0.21% and 21.03±0.01 to 23.08±0.03% respectively.
28867105	3	16	theme	42.15±0.03	430:439	arg1	%					440:440	47.06±0.01 and 42.15±0.03%	415:440	%	440:440	The starch content of cookie ranged between 47.06±0.01 and 42.15±0.03% and dietary fiber was reported highest in CSH14 (9.27±0.01%).
28867105	6	17	theme	dietary	812:818	arg1	fiber					820:824	dietary fiber	812:824	dietary fiber	812:824	The sensory scores for overall acceptability of cookies were highest in CSH23, CSH13R and CSV18R cultivars which are rich in dietary fiber and minerals.
28867105	8	18	theme	identified	975:984	arg1	cultivars					986:994	two identified cultivars	971:994	two identified cultivars (CSH23 & CSH13R)	971:1011	It is implied the raw material costs of two identified cultivars (CSH23 & CSH13R) would help the industry to reduce overall cost of production and offer a better profit margins over the varieties.
28867105	8	18	theme	identified	975:984	arg1	CSH13R					1005:1010	CSH23 & CSH13R	997:1010	CSH23 & CSH13R	997:1010	It is implied the raw material costs of two identified cultivars (CSH23 & CSH13R) would help the industry to reduce overall cost of production and offer a better profit margins over the varieties.
28867105	6	19	theme	cookies	735:741	arg1	acceptability					718:730	overall acceptability	710:730	overall acceptability of cookies	710:741	The sensory scores for overall acceptability of cookies were highest in CSH23, CSH13R and CSV18R cultivars which are rich in dietary fiber and minerals.
28867105	2	20	dep	8.27±0.21	316:324	arg1	to					313:314	to	313:314	to	313:314	Protein and fat content of cookies were ranged from 5.89±0.04 to 8.27±0.21% and 21.03±0.01 to 23.08±0.03% respectively.
28867105	1	21	theme	suitable	160:167	arg1	cultivars					150:158	best cultivars	145:158	best cultivars suitable for sorghum cookies accordingly nutrient	145:208	The study aimed to identify best cultivars suitable for sorghum cookies accordingly nutrient and mineral compositions were evaluated.
28867105	3	22	theme	starch	375:380	arg1	content					382:388	The starch content	371:388	The starch content of cookie ranged between 47.06±0.01 and 42.15±0.03% and dietary fiber	371:458	The starch content of cookie ranged between 47.06±0.01 and 42.15±0.03% and dietary fiber was reported highest in CSH14 (9.27±0.01%).
28867105	3	23	theme	47.06±0.01	415:424	arg1	%					440:440	47.06±0.01 and 42.15±0.03%	415:440	%	440:440	The starch content of cookie ranged between 47.06±0.01 and 42.15±0.03% and dietary fiber was reported highest in CSH14 (9.27±0.01%).
28867105	8	24	theme	better	1086:1091	arg1	profit					1093:1098	a better profit	1084:1098	a better profit	1084:1098	It is implied the raw material costs of two identified cultivars (CSH23 & CSH13R) would help the industry to reduce overall cost of production and offer a better profit margins over the varieties.
28867105	4	25	theme	Mg	516:517	arg1	255.54±0.03mg/100g					542:559	255.54±0.03mg/100g	542:559	255.54±0.03mg/100g	542:559	The highest Mg (56.24±0.03mg/100g) P (255.54±0.03mg/100g), and K (124.26±0.02mg/100g) content were found in C43 cultivar.
28867105	4	25	theme	Mg	516:517	arg1	P					539:539	Mg (56.24±0.03mg/100g) P	516:539	Mg (56.24±0.03mg/100g) P (255.54±0.03mg/100g)	516:560	The highest Mg (56.24±0.03mg/100g) P (255.54±0.03mg/100g), and K (124.26±0.02mg/100g) content were found in C43 cultivar.
28867105	5	26	theme	highest	646:652	arg1	1.23±0.01mg/100g					668:683	1.23±0.01mg/100g	668:683	1.23±0.01mg/100g	668:683	CSV18R was reported highest iron content (1.23±0.01mg/100g).
28867105	5	26	theme	highest	646:652	arg1	content					659:665	highest iron content	646:665	highest iron content (1.23±0.01mg/100g)	646:684	CSV18R was reported highest iron content (1.23±0.01mg/100g).
28867105	7	27	theme	high	865:868	arg1	yielders					870:877	high yielders	865:877	high yielders	865:877	Normally the hybrids are high yielders and the grain price/qt is 20% lower than varieties.
28867105	7	27	theme	high	865:868	arg1	hybrids					853:859	the hybrids	849:859	the hybrids	849:859	Normally the hybrids are high yielders and the grain price/qt is 20% lower than varieties.
28867105	1	28	theme	sorghum	173:179	arg1	cookies					181:187	sorghum cookies	173:187	sorghum cookies accordingly nutrient	173:208	The study aimed to identify best cultivars suitable for sorghum cookies accordingly nutrient and mineral compositions were evaluated.
28867105	6	29	from	rich	804:807	arg1	minerals					830:837	minerals	830:837	minerals	830:837	The sensory scores for overall acceptability of cookies were highest in CSH23, CSH13R and CSV18R cultivars which are rich in dietary fiber and minerals.
28867105	6	29	from	rich	804:807	arg1	fiber					820:824	dietary fiber	812:824	dietary fiber	812:824	The sensory scores for overall acceptability of cookies were highest in CSH23, CSH13R and CSV18R cultivars which are rich in dietary fiber and minerals.
28867105	0	30	theme	sorghum	98:104	arg1	cultivars					106:114	12 sorghum cultivars	95:114	12 sorghum cultivars	95:114	Study on evaluation of starch, dietary fiber and mineral composition of cookies developed from 12 sorghum cultivars.
28867105	4	31	located	found	603:607	arg2	content					590:596	The highest Mg (56.24±0.03mg/100g) P (255.54±0.03mg/100g), and K (124.26±0.02mg/100g) content	504:596	The highest Mg (56.24±0.03mg/100g) P (255.54±0.03mg/100g), and K (124.26±0.02mg/100g) content	504:596	The highest Mg (56.24±0.03mg/100g) P (255.54±0.03mg/100g), and K (124.26±0.02mg/100g) content were found in C43 cultivar.
28867105	4	31	located	found	603:607	arg1	cultivar					616:623	C43 cultivar	612:623	C43 cultivar	612:623	The highest Mg (56.24±0.03mg/100g) P (255.54±0.03mg/100g), and K (124.26±0.02mg/100g) content were found in C43 cultivar.
28867105	3	32	theme	cookie	393:398	arg1	content					382:388	The starch content	371:388	The starch content of cookie ranged between 47.06±0.01 and 42.15±0.03% and dietary fiber	371:458	The starch content of cookie ranged between 47.06±0.01 and 42.15±0.03% and dietary fiber was reported highest in CSH14 (9.27±0.01%).
28867105	4	33	theme	56.24±0.03mg/100g	520:536	arg1	255.54±0.03mg/100g					542:559	255.54±0.03mg/100g	542:559	255.54±0.03mg/100g	542:559	The highest Mg (56.24±0.03mg/100g) P (255.54±0.03mg/100g), and K (124.26±0.02mg/100g) content were found in C43 cultivar.
28867105	4	33	theme	56.24±0.03mg/100g	520:536	arg1	P					539:539	Mg (56.24±0.03mg/100g) P	516:539	Mg (56.24±0.03mg/100g) P (255.54±0.03mg/100g)	516:560	The highest Mg (56.24±0.03mg/100g) P (255.54±0.03mg/100g), and K (124.26±0.02mg/100g) content were found in C43 cultivar.
28867105	8	34	theme	&	1003:1003	arg1	cultivars					986:994	two identified cultivars	971:994	two identified cultivars (CSH23 & CSH13R)	971:1011	It is implied the raw material costs of two identified cultivars (CSH23 & CSH13R) would help the industry to reduce overall cost of production and offer a better profit margins over the varieties.
28867105	8	34	theme	&	1003:1003	arg1	CSH13R					1005:1010	CSH23 & CSH13R	997:1010	CSH23 & CSH13R	997:1010	It is implied the raw material costs of two identified cultivars (CSH23 & CSH13R) would help the industry to reduce overall cost of production and offer a better profit margins over the varieties.
28867105	8	35	theme	cultivars	986:994	arg1	costs					962:966	the raw material costs	945:966	the raw material costs of two identified cultivars (CSH23 & CSH13R)	945:1011	It is implied the raw material costs of two identified cultivars (CSH23 & CSH13R) would help the industry to reduce overall cost of production and offer a better profit margins over the varieties.
28867105	4	36	theme	C43	612:614	arg1	cultivar					616:623	C43 cultivar	612:623	C43 cultivar	612:623	The highest Mg (56.24±0.03mg/100g) P (255.54±0.03mg/100g), and K (124.26±0.02mg/100g) content were found in C43 cultivar.
28867105	0	37	theme	starch	23:28	arg1	evaluation					9:18	evaluation	9:18	evaluation of starch, dietary fiber and mineral composition of cookies	9:78	Study on evaluation of starch, dietary fiber and mineral composition of cookies developed from 12 sorghum cultivars.
28867105	6	38	theme	overall	710:716	arg1	acceptability					718:730	overall acceptability	710:730	overall acceptability of cookies	710:741	The sensory scores for overall acceptability of cookies were highest in CSH23, CSH13R and CSV18R cultivars which are rich in dietary fiber and minerals.
28867105	1	39	theme	nutrient	201:208	arg1	cookies					181:187	sorghum cookies	173:187	sorghum cookies accordingly nutrient	173:208	The study aimed to identify best cultivars suitable for sorghum cookies accordingly nutrient and mineral compositions were evaluated.
28867105	0	40	theme	fiber	39:43	arg1	evaluation					9:18	evaluation	9:18	evaluation of starch, dietary fiber and mineral composition of cookies	9:78	Study on evaluation of starch, dietary fiber and mineral composition of cookies developed from 12 sorghum cultivars.
28867105	0	41	theme	dietary	31:37	arg1	fiber					39:43	dietary fiber	31:43	dietary fiber	31:43	Study on evaluation of starch, dietary fiber and mineral composition of cookies developed from 12 sorghum cultivars.
28867105	4	42	theme	highest	508:514	arg1	content					590:596	The highest Mg (56.24±0.03mg/100g) P (255.54±0.03mg/100g), and K (124.26±0.02mg/100g) content	504:596	The highest Mg (56.24±0.03mg/100g) P (255.54±0.03mg/100g), and K (124.26±0.02mg/100g) content	504:596	The highest Mg (56.24±0.03mg/100g) P (255.54±0.03mg/100g), and K (124.26±0.02mg/100g) content were found in C43 cultivar.
28867105	6	43	theme	CSV18R	777:782	arg1	cultivars					784:792	CSV18R cultivars	777:792	CSV18R cultivars	777:792	The sensory scores for overall acceptability of cookies were highest in CSH23, CSH13R and CSV18R cultivars which are rich in dietary fiber and minerals.
28867105	6	44	theme	sensory	691:697	arg1	scores					699:704	The sensory scores	687:704	The sensory scores for overall acceptability of cookies	687:741	The sensory scores for overall acceptability of cookies were highest in CSH23, CSH13R and CSV18R cultivars which are rich in dietary fiber and minerals.
28867105	6	44	theme	sensory	691:697	arg1	highest					748:754	highest	748:754	highest	748:754	The sensory scores for overall acceptability of cookies were highest in CSH23, CSH13R and CSV18R cultivars which are rich in dietary fiber and minerals.
28867105	2	45	dep	23.08±0.03	345:354	arg1	to					342:343	to	342:343	to	342:343	Protein and fat content of cookies were ranged from 5.89±0.04 to 8.27±0.21% and 21.03±0.01 to 23.08±0.03% respectively.
28867105	2	46	theme	Protein	251:257	arg1	content					267:273	Protein and fat content	251:273	content	267:273	Protein and fat content of cookies were ranged from 5.89±0.04 to 8.27±0.21% and 21.03±0.01 to 23.08±0.03% respectively.
28867105	1	47	theme	mineral	214:220	arg1	compositions					222:233	mineral compositions	214:233	mineral compositions	214:233	The study aimed to identify best cultivars suitable for sorghum cookies accordingly nutrient and mineral compositions were evaluated.
28867105	0	48	theme	mineral	49:55	arg1	composition					57:67	mineral composition	49:67	mineral composition	49:67	Study on evaluation of starch, dietary fiber and mineral composition of cookies developed from 12 sorghum cultivars.
28867105	3	49	theme	dietary	446:452	arg1	fiber					454:458	dietary fiber	446:458	dietary fiber	446:458	The starch content of cookie ranged between 47.06±0.01 and 42.15±0.03% and dietary fiber was reported highest in CSH14 (9.27±0.01%).
28867105	4	50	theme	K	567:567	arg1	content					590:596	The highest Mg (56.24±0.03mg/100g) P (255.54±0.03mg/100g), and K (124.26±0.02mg/100g) content	504:596	The highest Mg (56.24±0.03mg/100g) P (255.54±0.03mg/100g), and K (124.26±0.02mg/100g) content	504:596	The highest Mg (56.24±0.03mg/100g) P (255.54±0.03mg/100g), and K (124.26±0.02mg/100g) content were found in C43 cultivar.
26616947	0	0	with	films	34:38	arg1	performance					65:75	novel antimicrobial performance	45:75	novel antimicrobial performance	45:75	Green and biodegradable composite films with novel antimicrobial performance based on cellulose.
26616947	5	1	theme	green	953:957	arg1	materials					959:967	green materials	953:967	green materials for food packaging	953:986	The results presented a new insight into the development of green materials for food packaging.
26616947	5	2	theme	food	973:976	arg1	packaging					978:986	food packaging	973:986	food packaging	973:986	The results presented a new insight into the development of green materials for food packaging.
26616947	1	3	theme	antimicrobial	155:167	arg1	properties					169:178	antimicrobial properties	155:178	antimicrobial properties	155:178	In order to obtain a safe and biodegradable material with antimicrobial properties from cellulose for food packaging, we presented a facile way to graft chitosan onto the oxidized cellulose films.
26616947	1	4	theme	graft	244:248	arg1	chitosan					250:257	graft chitosan	244:257	graft chitosan	244:257	In order to obtain a safe and biodegradable material with antimicrobial properties from cellulose for food packaging, we presented a facile way to graft chitosan onto the oxidized cellulose films.
26616947	3	5	theme	food-packaging	662:675	arg1	applicability					645:657	their applicability	639:657	their applicability	639:657	The antimicrobial properties, mechanical properties, and water vapor permeability of composites are essential characteristics in determining their applicability as food-packaging materials.
26616947	3	5	theme	food-packaging	662:675	arg1	materials					677:685	food-packaging materials	662:685	food-packaging materials	662:685	The antimicrobial properties, mechanical properties, and water vapor permeability of composites are essential characteristics in determining their applicability as food-packaging materials.
26616947	3	6	theme	water	555:559	arg1	properties					516:525	The antimicrobial properties	498:525	The antimicrobial properties	498:525	The antimicrobial properties, mechanical properties, and water vapor permeability of composites are essential characteristics in determining their applicability as food-packaging materials.
26616947	3	6	theme	water	555:559	arg1	characteristics					608:622	essential characteristics	598:622	essential characteristics	598:622	The antimicrobial properties, mechanical properties, and water vapor permeability of composites are essential characteristics in determining their applicability as food-packaging materials.
26616947	3	6	theme	water	555:559	arg1	permeability					567:578	water vapor permeability	555:578	water vapor permeability	555:578	The antimicrobial properties, mechanical properties, and water vapor permeability of composites are essential characteristics in determining their applicability as food-packaging materials.
26616947	3	6	theme	water	555:559	arg1	properties					539:548	mechanical properties	528:548	mechanical properties	528:548	The antimicrobial properties, mechanical properties, and water vapor permeability of composites are essential characteristics in determining their applicability as food-packaging materials.
26616947	1	7	with	material	141:148	arg1	properties					169:178	antimicrobial properties	155:178	antimicrobial properties	155:178	In order to obtain a safe and biodegradable material with antimicrobial properties from cellulose for food packaging, we presented a facile way to graft chitosan onto the oxidized cellulose films.
26616947	3	8	theme	vapor	561:565	arg1	properties					516:525	The antimicrobial properties	498:525	The antimicrobial properties	498:525	The antimicrobial properties, mechanical properties, and water vapor permeability of composites are essential characteristics in determining their applicability as food-packaging materials.
26616947	3	8	theme	vapor	561:565	arg1	characteristics					608:622	essential characteristics	598:622	essential characteristics	598:622	The antimicrobial properties, mechanical properties, and water vapor permeability of composites are essential characteristics in determining their applicability as food-packaging materials.
26616947	3	8	theme	vapor	561:565	arg1	permeability					567:578	water vapor permeability	555:578	water vapor permeability	555:578	The antimicrobial properties, mechanical properties, and water vapor permeability of composites are essential characteristics in determining their applicability as food-packaging materials.
26616947	3	8	theme	vapor	561:565	arg1	properties					539:548	mechanical properties	528:548	mechanical properties	528:548	The antimicrobial properties, mechanical properties, and water vapor permeability of composites are essential characteristics in determining their applicability as food-packaging materials.
26616947	4	9	theme	better	764:769	arg1	performance					771:781	better performance	764:781	better performance	764:781	Moreover, using a sausage model, it was shown that the composites exhibited better performance than traditional polyethylene packaging material and demonstrated good potential as food packaging materials.
26616947	2	10	theme	excellent	373:381	arg1	properties					391:400	excellent barrier properties	373:400	excellent barrier properties against oxygen	373:415	The obtained films had a high transparent property of above 80% transmittance, excellent barrier properties against oxygen and antimicrobial properties against Escherichia coli and Staphylococcus aureus.
26616947	3	11	theme	composites	583:592	arg1	characteristics					608:622	essential characteristics	598:622	essential characteristics	598:622	The antimicrobial properties, mechanical properties, and water vapor permeability of composites are essential characteristics in determining their applicability as food-packaging materials.
26616947	3	11	theme	composites	583:592	arg1	permeability					567:578	water vapor permeability	555:578	water vapor permeability	555:578	The antimicrobial properties, mechanical properties, and water vapor permeability of composites are essential characteristics in determining their applicability as food-packaging materials.
26616947	3	11	theme	composites	583:592	arg1	properties					516:525	The antimicrobial properties	498:525	The antimicrobial properties	498:525	The antimicrobial properties, mechanical properties, and water vapor permeability of composites are essential characteristics in determining their applicability as food-packaging materials.
26616947	3	11	theme	composites	583:592	arg1	properties					539:548	mechanical properties	528:548	mechanical properties	528:548	The antimicrobial properties, mechanical properties, and water vapor permeability of composites are essential characteristics in determining their applicability as food-packaging materials.
26616947	2	12	theme	transmittance	358:370	arg1	properties					435:444	antimicrobial properties	421:444	antimicrobial properties against Escherichia coli and Staphylococcus aureus	421:495	The obtained films had a high transparent property of above 80% transmittance, excellent barrier properties against oxygen and antimicrobial properties against Escherichia coli and Staphylococcus aureus.
26616947	2	12	theme	transmittance	358:370	arg1	properties					391:400	excellent barrier properties	373:400	excellent barrier properties against oxygen	373:415	The obtained films had a high transparent property of above 80% transmittance, excellent barrier properties against oxygen and antimicrobial properties against Escherichia coli and Staphylococcus aureus.
26616947	2	12	theme	transmittance	358:370	arg1	property					336:343	a high transparent property	317:343	a high transparent property of above 80% transmittance	317:370	The obtained films had a high transparent property of above 80% transmittance, excellent barrier properties against oxygen and antimicrobial properties against Escherichia coli and Staphylococcus aureus.
26616947	0	13	theme	Green	0:4	arg1	films					34:38	Green and biodegradable composite films	0:38	Green and biodegradable composite films with novel antimicrobial performance	0:75	Green and biodegradable composite films with novel antimicrobial performance based on cellulose.
26616947	2	14	theme	obtained	298:305	arg1	films					307:311	The obtained films	294:311	The obtained films	294:311	The obtained films had a high transparent property of above 80% transmittance, excellent barrier properties against oxygen and antimicrobial properties against Escherichia coli and Staphylococcus aureus.
26616947	4	15	theme	packaging	813:821	arg1	material					823:830	traditional polyethylene packaging material	788:830	traditional polyethylene packaging material	788:830	Moreover, using a sausage model, it was shown that the composites exhibited better performance than traditional polyethylene packaging material and demonstrated good potential as food packaging materials.
26616947	4	16	theme	sausage	706:712	arg1	model					714:718	a sausage model	704:718	a sausage model	704:718	Moreover, using a sausage model, it was shown that the composites exhibited better performance than traditional polyethylene packaging material and demonstrated good potential as food packaging materials.
26616947	2	17	theme	antimicrobial	421:433	arg1	properties					435:444	antimicrobial properties	421:444	antimicrobial properties against Escherichia coli and Staphylococcus aureus	421:495	The obtained films had a high transparent property of above 80% transmittance, excellent barrier properties against oxygen and antimicrobial properties against Escherichia coli and Staphylococcus aureus.
26616947	1	18	theme	oxidized	268:275	arg1	films					287:291	the oxidized cellulose films	264:291	the oxidized cellulose films	264:291	In order to obtain a safe and biodegradable material with antimicrobial properties from cellulose for food packaging, we presented a facile way to graft chitosan onto the oxidized cellulose films.
26616947	4	19	theme	traditional	788:798	arg1	material					823:830	traditional polyethylene packaging material	788:830	traditional polyethylene packaging material	788:830	Moreover, using a sausage model, it was shown that the composites exhibited better performance than traditional polyethylene packaging material and demonstrated good potential as food packaging materials.
26616947	1	20	theme	food	199:202	arg1	packaging					204:212	food packaging	199:212	food packaging	199:212	In order to obtain a safe and biodegradable material with antimicrobial properties from cellulose for food packaging, we presented a facile way to graft chitosan onto the oxidized cellulose films.
26616947	0	21	theme	biodegradable	10:22	arg1	films					34:38	Green and biodegradable composite films	0:38	Green and biodegradable composite films with novel antimicrobial performance	0:75	Green and biodegradable composite films with novel antimicrobial performance based on cellulose.
26616947	1	22	theme	cellulose	277:285	arg1	films					287:291	the oxidized cellulose films	264:291	the oxidized cellulose films	264:291	In order to obtain a safe and biodegradable material with antimicrobial properties from cellulose for food packaging, we presented a facile way to graft chitosan onto the oxidized cellulose films.
26616947	5	23	theme	materials	959:967	arg1	development					938:948	the development	934:948	the development of green materials for food packaging	934:986	The results presented a new insight into the development of green materials for food packaging.
26616947	3	24	theme	mechanical	528:537	arg1	permeability					567:578	water vapor permeability	555:578	water vapor permeability	555:578	The antimicrobial properties, mechanical properties, and water vapor permeability of composites are essential characteristics in determining their applicability as food-packaging materials.
26616947	3	24	theme	mechanical	528:537	arg1	characteristics					608:622	essential characteristics	598:622	essential characteristics	598:622	The antimicrobial properties, mechanical properties, and water vapor permeability of composites are essential characteristics in determining their applicability as food-packaging materials.
26616947	3	24	theme	mechanical	528:537	arg1	properties					516:525	The antimicrobial properties	498:525	The antimicrobial properties	498:525	The antimicrobial properties, mechanical properties, and water vapor permeability of composites are essential characteristics in determining their applicability as food-packaging materials.
26616947	3	24	theme	mechanical	528:537	arg1	properties					539:548	mechanical properties	528:548	mechanical properties	528:548	The antimicrobial properties, mechanical properties, and water vapor permeability of composites are essential characteristics in determining their applicability as food-packaging materials.
26616947	3	25	theme	antimicrobial	502:514	arg1	permeability					567:578	water vapor permeability	555:578	water vapor permeability	555:578	The antimicrobial properties, mechanical properties, and water vapor permeability of composites are essential characteristics in determining their applicability as food-packaging materials.
26616947	3	25	theme	antimicrobial	502:514	arg1	characteristics					608:622	essential characteristics	598:622	essential characteristics	598:622	The antimicrobial properties, mechanical properties, and water vapor permeability of composites are essential characteristics in determining their applicability as food-packaging materials.
26616947	3	25	theme	antimicrobial	502:514	arg1	properties					516:525	The antimicrobial properties	498:525	The antimicrobial properties	498:525	The antimicrobial properties, mechanical properties, and water vapor permeability of composites are essential characteristics in determining their applicability as food-packaging materials.
26616947	3	25	theme	antimicrobial	502:514	arg1	properties					539:548	mechanical properties	528:548	mechanical properties	528:548	The antimicrobial properties, mechanical properties, and water vapor permeability of composites are essential characteristics in determining their applicability as food-packaging materials.
26616947	4	26	theme	packaging	872:880	arg1	potential					854:862	good potential	849:862	good potential	849:862	Moreover, using a sausage model, it was shown that the composites exhibited better performance than traditional polyethylene packaging material and demonstrated good potential as food packaging materials.
26616947	4	26	theme	packaging	872:880	arg1	materials					882:890	food packaging materials	867:890	food packaging materials	867:890	Moreover, using a sausage model, it was shown that the composites exhibited better performance than traditional polyethylene packaging material and demonstrated good potential as food packaging materials.
26616947	5	27	theme	new	917:919	arg1	insight					921:927	a new insight	915:927	a new insight into the development of green materials for food packaging	915:986	The results presented a new insight into the development of green materials for food packaging.
26616947	4	28	theme	polyethylene	800:811	arg1	material					823:830	traditional polyethylene packaging material	788:830	traditional polyethylene packaging material	788:830	Moreover, using a sausage model, it was shown that the composites exhibited better performance than traditional polyethylene packaging material and demonstrated good potential as food packaging materials.
26616947	0	29	theme	composite	24:32	arg1	films					34:38	Green and biodegradable composite films	0:38	Green and biodegradable composite films with novel antimicrobial performance	0:75	Green and biodegradable composite films with novel antimicrobial performance based on cellulose.
26616947	2	30	theme	barrier	383:389	arg1	properties					391:400	excellent barrier properties	373:400	excellent barrier properties against oxygen	373:415	The obtained films had a high transparent property of above 80% transmittance, excellent barrier properties against oxygen and antimicrobial properties against Escherichia coli and Staphylococcus aureus.
26616947	0	31	theme	antimicrobial	51:63	arg1	performance					65:75	novel antimicrobial performance	45:75	novel antimicrobial performance	45:75	Green and biodegradable composite films with novel antimicrobial performance based on cellulose.
26616947	3	32	theme	essential	598:606	arg1	permeability					567:578	water vapor permeability	555:578	water vapor permeability	555:578	The antimicrobial properties, mechanical properties, and water vapor permeability of composites are essential characteristics in determining their applicability as food-packaging materials.
26616947	3	32	theme	essential	598:606	arg1	characteristics					608:622	essential characteristics	598:622	essential characteristics	598:622	The antimicrobial properties, mechanical properties, and water vapor permeability of composites are essential characteristics in determining their applicability as food-packaging materials.
26616947	3	32	theme	essential	598:606	arg1	properties					516:525	The antimicrobial properties	498:525	The antimicrobial properties	498:525	The antimicrobial properties, mechanical properties, and water vapor permeability of composites are essential characteristics in determining their applicability as food-packaging materials.
26616947	3	32	theme	essential	598:606	arg1	properties					539:548	mechanical properties	528:548	mechanical properties	528:548	The antimicrobial properties, mechanical properties, and water vapor permeability of composites are essential characteristics in determining their applicability as food-packaging materials.
26616947	2	33	theme	transparent	324:334	arg1	property					336:343	a high transparent property	317:343	a high transparent property of above 80% transmittance	317:370	The obtained films had a high transparent property of above 80% transmittance, excellent barrier properties against oxygen and antimicrobial properties against Escherichia coli and Staphylococcus aureus.
26616947	0	34	theme	novel	45:49	arg1	performance					65:75	novel antimicrobial performance	45:75	novel antimicrobial performance	45:75	Green and biodegradable composite films with novel antimicrobial performance based on cellulose.
26616947	2	35	theme	high	319:322	arg1	property					336:343	a high transparent property	317:343	a high transparent property of above 80% transmittance	317:370	The obtained films had a high transparent property of above 80% transmittance, excellent barrier properties against oxygen and antimicrobial properties against Escherichia coli and Staphylococcus aureus.
26616947	4	36	theme	good	849:852	arg1	potential					854:862	good potential	849:862	good potential	849:862	Moreover, using a sausage model, it was shown that the composites exhibited better performance than traditional polyethylene packaging material and demonstrated good potential as food packaging materials.
26616947	4	36	theme	good	849:852	arg1	materials					882:890	food packaging materials	867:890	food packaging materials	867:890	Moreover, using a sausage model, it was shown that the composites exhibited better performance than traditional polyethylene packaging material and demonstrated good potential as food packaging materials.
26616947	2	37	dep	transmittance	358:370	arg1	%					356:356	%	356:356	%	356:356	The obtained films had a high transparent property of above 80% transmittance, excellent barrier properties against oxygen and antimicrobial properties against Escherichia coli and Staphylococcus aureus.
26616947	4	38	theme	food	867:870	arg1	potential					854:862	good potential	849:862	good potential	849:862	Moreover, using a sausage model, it was shown that the composites exhibited better performance than traditional polyethylene packaging material and demonstrated good potential as food packaging materials.
26616947	4	38	theme	food	867:870	arg1	materials					882:890	food packaging materials	867:890	food packaging materials	867:890	Moreover, using a sausage model, it was shown that the composites exhibited better performance than traditional polyethylene packaging material and demonstrated good potential as food packaging materials.
26616947	1	39	theme	safe	118:121	arg1	material					141:148	a safe and biodegradable material	116:148	a safe and biodegradable material with antimicrobial properties from cellulose	116:193	In order to obtain a safe and biodegradable material with antimicrobial properties from cellulose for food packaging, we presented a facile way to graft chitosan onto the oxidized cellulose films.
26616947	2	40	dep	%	356:356	arg1	80					354:355	80	354:355	80	354:355	The obtained films had a high transparent property of above 80% transmittance, excellent barrier properties against oxygen and antimicrobial properties against Escherichia coli and Staphylococcus aureus.
26616947	1	41	from	cellulose	185:193	arg1	material					141:148	a safe and biodegradable material	116:148	a safe and biodegradable material with antimicrobial properties from cellulose	116:193	In order to obtain a safe and biodegradable material with antimicrobial properties from cellulose for food packaging, we presented a facile way to graft chitosan onto the oxidized cellulose films.
26616947	1	42	theme	biodegradable	127:139	arg1	material					141:148	a safe and biodegradable material	116:148	a safe and biodegradable material with antimicrobial properties from cellulose	116:193	In order to obtain a safe and biodegradable material with antimicrobial properties from cellulose for food packaging, we presented a facile way to graft chitosan onto the oxidized cellulose films.
26616947	1	43	theme	facile	230:235	arg1	way					237:239	a facile way	228:239	a facile way to graft chitosan	228:257	In order to obtain a safe and biodegradable material with antimicrobial properties from cellulose for food packaging, we presented a facile way to graft chitosan onto the oxidized cellulose films.
26616947	2	44	contain	had	313:315	arg2	properties					391:400	excellent barrier properties	373:400	excellent barrier properties against oxygen	373:415	The obtained films had a high transparent property of above 80% transmittance, excellent barrier properties against oxygen and antimicrobial properties against Escherichia coli and Staphylococcus aureus.
26616947	2	44	contain	had	313:315	arg2	properties					435:444	antimicrobial properties	421:444	antimicrobial properties against Escherichia coli and Staphylococcus aureus	421:495	The obtained films had a high transparent property of above 80% transmittance, excellent barrier properties against oxygen and antimicrobial properties against Escherichia coli and Staphylococcus aureus.
26616947	2	44	contain	had	313:315	arg2	property					336:343	a high transparent property	317:343	a high transparent property of above 80% transmittance	317:370	The obtained films had a high transparent property of above 80% transmittance, excellent barrier properties against oxygen and antimicrobial properties against Escherichia coli and Staphylococcus aureus.
26616947	2	44	contain	had	313:315	arg1	films					307:311	The obtained films	294:311	The obtained films	294:311	The obtained films had a high transparent property of above 80% transmittance, excellent barrier properties against oxygen and antimicrobial properties against Escherichia coli and Staphylococcus aureus.
28176356	7	0	theme	significant	1303:1313	arg1	changes					1315:1321	significant changes	1303:1321	significant changes in collagen distribution and alignment with maturity	1303:1374	These observations reveal significant changes in collagen distribution and alignment with maturity, and these trends underscore the role of physiologic loading in postnatal matrix remodeling.
28176356	7	1	theme	physiologic	1417:1427	arg1	loading					1429:1435	physiologic loading	1417:1435	physiologic loading	1417:1435	These observations reveal significant changes in collagen distribution and alignment with maturity, and these trends underscore the role of physiologic loading in postnatal matrix remodeling.
28176356	7	2	with	distribution	1335:1346	arg1	maturity					1367:1374	maturity	1367:1374	maturity	1367:1374	These observations reveal significant changes in collagen distribution and alignment with maturity, and these trends underscore the role of physiologic loading in postnatal matrix remodeling.
28176356	3	3	theme	organization	548:559	arg1	understanding					504:516	quantitative understanding	491:516	quantitative understanding of the matrix composition and organization across this junction	491:580	Presently, there is a lack of quantitative understanding of the matrix composition and organization across this junction, especially after the onset of skeletal maturity.
28176356	8	4	theme	integrative	1613:1623	arg1	repair					1637:1642	integrative soft tissue repair	1613:1642	integrative soft tissue repair	1613:1642	Findings from this study provide new insights into interface organization and serve as benchmark design criteria for interface regeneration and integrative soft tissue repair.
28176356	4	5	theme	spectroscopic	751:763	arg1	FTIRI					774:778	FTIRI	774:778	FTIRI	774:778	The objective of this study is to characterize the adult bovine ACL-to-bone interface using Fourier transform infrared spectroscopic imaging (FTIRI), testing the hypothesis that regional changes in collagen, proteoglycan, and mineral distribution, as well as matrix organization, persist at the mature insertion.
28176356	4	5	theme	spectroscopic	751:763	arg1	imaging					765:771	spectroscopic imaging	751:771	spectroscopic imaging (FTIRI)	751:779	The objective of this study is to characterize the adult bovine ACL-to-bone interface using Fourier transform infrared spectroscopic imaging (FTIRI), testing the hypothesis that regional changes in collagen, proteoglycan, and mineral distribution, as well as matrix organization, persist at the mature insertion.
28176356	8	6	theme	tissue	1630:1635	arg1	repair					1637:1642	integrative soft tissue repair	1613:1642	integrative soft tissue repair	1613:1642	Findings from this study provide new insights into interface organization and serve as benchmark design criteria for interface regeneration and integrative soft tissue repair.
28176356	1	7	theme	bone	281:284	arg1	regions					286:292	bone regions	281:292	bone regions	281:292	The anterior cruciate ligament (ACL)-to-bone interface constitutes a complex, multi-tissue structure comprised of contiguous ligament, non-mineralized fibrocartilage, mineralized fibrocartilage, and bone regions.
28176356	7	8	theme	loading	1429:1435	arg1	role					1409:1412	the role	1405:1412	the role of physiologic loading in postnatal matrix remodeling	1405:1466	These observations reveal significant changes in collagen distribution and alignment with maturity, and these trends underscore the role of physiologic loading in postnatal matrix remodeling.
28176356	7	9	with	alignment	1352:1360	arg1	maturity					1367:1374	maturity	1367:1374	maturity	1367:1374	These observations reveal significant changes in collagen distribution and alignment with maturity, and these trends underscore the role of physiologic loading in postnatal matrix remodeling.
28176356	6	10	theme	non-mineralized	1236:1250	arg1	regions					1268:1274	the non-mineralized and mineralized regions	1232:1274	the non-mineralized and mineralized regions	1232:1274	Proteoglycans were primarily localized to the fibrocartilage region and an exponential increase in mineral content was observed between the non-mineralized and mineralized regions.
28176356	2	11	theme	load	328:331	arg1	transfer					333:340	load transfer	328:340	load transfer between structurally and functionally dissimilar tissues	328:397	This composite structure enables load transfer between structurally and functionally dissimilar tissues and is critical for ligament homeostasis and joint stability.
28176356	3	12	theme	skeletal	613:620	arg1	maturity					622:629	skeletal maturity	613:629	skeletal maturity	613:629	Presently, there is a lack of quantitative understanding of the matrix composition and organization across this junction, especially after the onset of skeletal maturity.
28176356	4	13	from	organization	898:909	arg1	distribution					866:877	mineral distribution	858:877	mineral distribution	858:877	The objective of this study is to characterize the adult bovine ACL-to-bone interface using Fourier transform infrared spectroscopic imaging (FTIRI), testing the hypothesis that regional changes in collagen, proteoglycan, and mineral distribution, as well as matrix organization, persist at the mature insertion.
28176356	4	13	from	organization	898:909	arg1	proteoglycan					840:851	proteoglycan	840:851	proteoglycan	840:851	The objective of this study is to characterize the adult bovine ACL-to-bone interface using Fourier transform infrared spectroscopic imaging (FTIRI), testing the hypothesis that regional changes in collagen, proteoglycan, and mineral distribution, as well as matrix organization, persist at the mature insertion.
28176356	4	13	from	organization	898:909	arg1	collagen					830:837	collagen	830:837	collagen	830:837	The objective of this study is to characterize the adult bovine ACL-to-bone interface using Fourier transform infrared spectroscopic imaging (FTIRI), testing the hypothesis that regional changes in collagen, proteoglycan, and mineral distribution, as well as matrix organization, persist at the mature insertion.
28176356	6	14	theme	mineralized	1256:1266	arg1	regions					1268:1274	the non-mineralized and mineralized regions	1232:1274	the non-mineralized and mineralized regions	1232:1274	Proteoglycans were primarily localized to the fibrocartilage region and an exponential increase in mineral content was observed between the non-mineralized and mineralized regions.
28176356	4	15	from	changes	819:825	arg1	distribution					866:877	mineral distribution	858:877	mineral distribution	858:877	The objective of this study is to characterize the adult bovine ACL-to-bone interface using Fourier transform infrared spectroscopic imaging (FTIRI), testing the hypothesis that regional changes in collagen, proteoglycan, and mineral distribution, as well as matrix organization, persist at the mature insertion.
28176356	4	15	from	changes	819:825	arg1	proteoglycan					840:851	proteoglycan	840:851	proteoglycan	840:851	The objective of this study is to characterize the adult bovine ACL-to-bone interface using Fourier transform infrared spectroscopic imaging (FTIRI), testing the hypothesis that regional changes in collagen, proteoglycan, and mineral distribution, as well as matrix organization, persist at the mature insertion.
28176356	4	15	from	changes	819:825	arg1	collagen					830:837	collagen	830:837	collagen	830:837	The objective of this study is to characterize the adult bovine ACL-to-bone interface using Fourier transform infrared spectroscopic imaging (FTIRI), testing the hypothesis that regional changes in collagen, proteoglycan, and mineral distribution, as well as matrix organization, persist at the mature insertion.
28176356	4	16	theme	regional	810:817	arg1	changes					819:825	regional changes	810:825	regional changes in collagen, proteoglycan, and mineral distribution	810:877	The objective of this study is to characterize the adult bovine ACL-to-bone interface using Fourier transform infrared spectroscopic imaging (FTIRI), testing the hypothesis that regional changes in collagen, proteoglycan, and mineral distribution, as well as matrix organization, persist at the mature insertion.
28176356	6	17	theme	fibrocartilage	1142:1155	arg1	region					1157:1162	the fibrocartilage region	1138:1162	the fibrocartilage region	1138:1162	Proteoglycans were primarily localized to the fibrocartilage region and an exponential increase in mineral content was observed between the non-mineralized and mineralized regions.
28176356	2	18	theme	composite	300:308	arg1	structure					310:318	This composite structure	295:318	This composite structure	295:318	This composite structure enables load transfer between structurally and functionally dissimilar tissues and is critical for ligament homeostasis and joint stability.
28176356	2	19	theme	joint	444:448	arg1	stability					450:458	joint stability	444:458	joint stability	444:458	This composite structure enables load transfer between structurally and functionally dissimilar tissues and is critical for ligament homeostasis and joint stability.
28176356	1	20	theme	anterior	86:93	arg1	interface					127:135	The anterior cruciate ligament (ACL)-to-bone interface	82:135	The anterior cruciate ligament (ACL)-to-bone interface	82:135	The anterior cruciate ligament (ACL)-to-bone interface constitutes a complex, multi-tissue structure comprised of contiguous ligament, non-mineralized fibrocartilage, mineralized fibrocartilage, and bone regions.
28176356	4	21	theme	ACL-to-bone	696:706	arg1	interface					708:716	the adult bovine ACL-to-bone interface	679:716	the adult bovine ACL-to-bone interface using Fourier transform infrared spectroscopic imaging (FTIRI), testing the hypothesis that regional changes in collagen, proteoglycan, and mineral distribution, as well as matrix organization, persist at the mature insertion	679:942	The objective of this study is to characterize the adult bovine ACL-to-bone interface using Fourier transform infrared spectroscopic imaging (FTIRI), testing the hypothesis that regional changes in collagen, proteoglycan, and mineral distribution, as well as matrix organization, persist at the mature insertion.
28176356	8	22	theme	interface	1520:1528	arg1	organization					1530:1541	interface organization	1520:1541	interface organization	1520:1541	Findings from this study provide new insights into interface organization and serve as benchmark design criteria for interface regeneration and integrative soft tissue repair.
28176356	1	23	theme	cruciate	95:102	arg1	interface					127:135	The anterior cruciate ligament (ACL)-to-bone interface	82:135	The anterior cruciate ligament (ACL)-to-bone interface	82:135	The anterior cruciate ligament (ACL)-to-bone interface constitutes a complex, multi-tissue structure comprised of contiguous ligament, non-mineralized fibrocartilage, mineralized fibrocartilage, and bone regions.
28176356	6	24	theme	mineral	1195:1201	arg1	content					1203:1209	mineral content	1195:1209	mineral content	1195:1209	Proteoglycans were primarily localized to the fibrocartilage region and an exponential increase in mineral content was observed between the non-mineralized and mineralized regions.
28176356	0	25	theme	Compositional	0:12	arg1	mapping					14:20	Compositional mapping	0:20	Compositional mapping of the mature anterior cruciate	0:52	Compositional mapping of the mature anterior cruciate ligament-to-bone insertion.
28176356	4	26	theme	matrix	891:896	arg1	organization					898:909	matrix organization	891:909	matrix organization	891:909	The objective of this study is to characterize the adult bovine ACL-to-bone interface using Fourier transform infrared spectroscopic imaging (FTIRI), testing the hypothesis that regional changes in collagen, proteoglycan, and mineral distribution, as well as matrix organization, persist at the mature insertion.
28176356	3	27	theme	maturity	622:629	arg1	onset					604:608	the onset	600:608	the onset of skeletal maturity	600:629	Presently, there is a lack of quantitative understanding of the matrix composition and organization across this junction, especially after the onset of skeletal maturity.
28176356	1	28	theme	contiguous	196:205	arg1	ligament					207:214	contiguous ligament	196:214	contiguous ligament	196:214	The anterior cruciate ligament (ACL)-to-bone interface constitutes a complex, multi-tissue structure comprised of contiguous ligament, non-mineralized fibrocartilage, mineralized fibrocartilage, and bone regions.
28176356	8	29	theme	design	1566:1571	arg1	criteria					1573:1580	benchmark design criteria	1556:1580	benchmark design criteria for interface regeneration and integrative soft tissue repair	1556:1642	Findings from this study provide new insights into interface organization and serve as benchmark design criteria for interface regeneration and integrative soft tissue repair.
28176356	8	29	theme	design	1566:1571	arg1	Findings					1469:1476	Findings	1469:1476	Findings from this study	1469:1492	Findings from this study provide new insights into interface organization and serve as benchmark design criteria for interface regeneration and integrative soft tissue repair.
28176356	3	30	theme	understanding	504:516	arg1	lack					483:486	a lack	481:486	a lack of quantitative understanding of the matrix composition and organization across this junction	481:580	Presently, there is a lack of quantitative understanding of the matrix composition and organization across this junction, especially after the onset of skeletal maturity.
28176356	1	31	theme	ligament	104:111	arg1	interface					127:135	The anterior cruciate ligament (ACL)-to-bone interface	82:135	The anterior cruciate ligament (ACL)-to-bone interface	82:135	The anterior cruciate ligament (ACL)-to-bone interface constitutes a complex, multi-tissue structure comprised of contiguous ligament, non-mineralized fibrocartilage, mineralized fibrocartilage, and bone regions.
28176356	3	32	dep	composition	532:542	arg1	the					521:523	the	521:523	the	521:523	Presently, there is a lack of quantitative understanding of the matrix composition and organization across this junction, especially after the onset of skeletal maturity.
28176356	8	33	theme	benchmark	1556:1564	arg1	criteria					1573:1580	benchmark design criteria	1556:1580	benchmark design criteria for interface regeneration and integrative soft tissue repair	1556:1642	Findings from this study provide new insights into interface organization and serve as benchmark design criteria for interface regeneration and integrative soft tissue repair.
28176356	8	33	theme	benchmark	1556:1564	arg1	Findings					1469:1476	Findings	1469:1476	Findings from this study	1469:1492	Findings from this study provide new insights into interface organization and serve as benchmark design criteria for interface regeneration and integrative soft tissue repair.
28176356	1	34	theme	ACL	114:116	arg1	interface					127:135	The anterior cruciate ligament (ACL)-to-bone interface	82:135	The anterior cruciate ligament (ACL)-to-bone interface	82:135	The anterior cruciate ligament (ACL)-to-bone interface constitutes a complex, multi-tissue structure comprised of contiguous ligament, non-mineralized fibrocartilage, mineralized fibrocartilage, and bone regions.
28176356	9	35	theme	Orthopaedic	1652:1662	arg1	Research					1664:1671	© 2017 Orthopaedic Research	1645:1671	© 2017 Orthopaedic Research Society.	1645:1680	© 2017 Orthopaedic Research Society.
28176356	0	36	theme	anterior	36:43	arg1	cruciate					45:52	the mature anterior cruciate	25:52	the mature anterior cruciate	25:52	Compositional mapping of the mature anterior cruciate ligament-to-bone insertion.
28176356	2	37	theme	dissimilar	380:389	arg1	tissues					391:397	structurally and functionally dissimilar tissues	350:397	structurally and functionally dissimilar tissues	350:397	This composite structure enables load transfer between structurally and functionally dissimilar tissues and is critical for ligament homeostasis and joint stability.
28176356	4	38	theme	bovine	689:694	arg1	interface					708:716	the adult bovine ACL-to-bone interface	679:716	the adult bovine ACL-to-bone interface using Fourier transform infrared spectroscopic imaging (FTIRI), testing the hypothesis that regional changes in collagen, proteoglycan, and mineral distribution, as well as matrix organization, persist at the mature insertion	679:942	The objective of this study is to characterize the adult bovine ACL-to-bone interface using Fourier transform infrared spectroscopic imaging (FTIRI), testing the hypothesis that regional changes in collagen, proteoglycan, and mineral distribution, as well as matrix organization, persist at the mature insertion.
28176356	8	39	theme	new	1502:1504	arg1	insights					1506:1513	new insights	1502:1513	new insights into interface organization	1502:1541	Findings from this study provide new insights into interface organization and serve as benchmark design criteria for interface regeneration and integrative soft tissue repair.
28176356	2	40	theme	ligament	419:426	arg1	homeostasis					428:438	ligament homeostasis	419:438	ligament homeostasis	419:438	This composite structure enables load transfer between structurally and functionally dissimilar tissues and is critical for ligament homeostasis and joint stability.
28176356	1	41	theme	non-mineralized	217:231	arg1	fibrocartilage					233:246	non-mineralized fibrocartilage	217:246	non-mineralized fibrocartilage	217:246	The anterior cruciate ligament (ACL)-to-bone interface constitutes a complex, multi-tissue structure comprised of contiguous ligament, non-mineralized fibrocartilage, mineralized fibrocartilage, and bone regions.
28176356	0	42	theme	mature	29:34	arg1	cruciate					45:52	the mature anterior cruciate	25:52	the mature anterior cruciate	25:52	Compositional mapping of the mature anterior cruciate ligament-to-bone insertion.
28176356	4	43	theme	mineral	858:864	arg1	distribution					866:877	mineral distribution	858:877	mineral distribution	858:877	The objective of this study is to characterize the adult bovine ACL-to-bone interface using Fourier transform infrared spectroscopic imaging (FTIRI), testing the hypothesis that regional changes in collagen, proteoglycan, and mineral distribution, as well as matrix organization, persist at the mature insertion.
28176356	3	44	theme	quantitative	491:502	arg1	understanding					504:516	quantitative understanding	491:516	quantitative understanding of the matrix composition and organization across this junction	491:580	Presently, there is a lack of quantitative understanding of the matrix composition and organization across this junction, especially after the onset of skeletal maturity.
28176356	8	45	theme	interface	1586:1594	arg1	regeneration					1596:1607	interface regeneration	1586:1607	interface regeneration	1586:1607	Findings from this study provide new insights into interface organization and serve as benchmark design criteria for interface regeneration and integrative soft tissue repair.
28176356	9	46	theme	©	1645:1645	arg1	Research					1664:1671	© 2017 Orthopaedic Research	1645:1671	© 2017 Orthopaedic Research Society.	1645:1680	© 2017 Orthopaedic Research Society.
28176356	5	47	theme	adult	1023:1027	arg1	interface					1029:1037	the adult interface	1019:1037	the adult interface	1019:1037	It was observed that while collagen content increases continuously across the adult interface, collagen alignment decreases between ligament and bone.
28176356	6	48	from	increase	1183:1190	arg1	content					1203:1209	mineral content	1195:1209	mineral content	1195:1209	Proteoglycans were primarily localized to the fibrocartilage region and an exponential increase in mineral content was observed between the non-mineralized and mineralized regions.
28176356	3	49	theme	composition	532:542	arg1	understanding					504:516	quantitative understanding	491:516	quantitative understanding of the matrix composition and organization across this junction	491:580	Presently, there is a lack of quantitative understanding of the matrix composition and organization across this junction, especially after the onset of skeletal maturity.
28176356	0	50	theme	cruciate	45:52	arg1	mapping					14:20	Compositional mapping	0:20	Compositional mapping of the mature anterior cruciate	0:52	Compositional mapping of the mature anterior cruciate ligament-to-bone insertion.
28176356	4	51	theme	mature	927:932	arg1	insertion					934:942	the mature insertion	923:942	the mature insertion	923:942	The objective of this study is to characterize the adult bovine ACL-to-bone interface using Fourier transform infrared spectroscopic imaging (FTIRI), testing the hypothesis that regional changes in collagen, proteoglycan, and mineral distribution, as well as matrix organization, persist at the mature insertion.
28176356	1	52	dep	complex	151:157	arg1	multi-tissue					160:171	multi-tissue	160:171	multi-tissue	160:171	The anterior cruciate ligament (ACL)-to-bone interface constitutes a complex, multi-tissue structure comprised of contiguous ligament, non-mineralized fibrocartilage, mineralized fibrocartilage, and bone regions.
28176356	1	53	theme	-to-bone	118:125	arg1	interface					127:135	The anterior cruciate ligament (ACL)-to-bone interface	82:135	The anterior cruciate ligament (ACL)-to-bone interface	82:135	The anterior cruciate ligament (ACL)-to-bone interface constitutes a complex, multi-tissue structure comprised of contiguous ligament, non-mineralized fibrocartilage, mineralized fibrocartilage, and bone regions.
28176356	5	54	theme	collagen	972:979	arg1	content					981:987	collagen content	972:987	collagen content	972:987	It was observed that while collagen content increases continuously across the adult interface, collagen alignment decreases between ligament and bone.
28176356	7	55	from	changes	1315:1321	arg1	alignment					1352:1360	alignment	1352:1360	alignment with maturity	1352:1374	These observations reveal significant changes in collagen distribution and alignment with maturity, and these trends underscore the role of physiologic loading in postnatal matrix remodeling.
28176356	7	55	from	changes	1315:1321	arg1	distribution					1335:1346	collagen distribution	1326:1346	collagen distribution	1326:1346	These observations reveal significant changes in collagen distribution and alignment with maturity, and these trends underscore the role of physiologic loading in postnatal matrix remodeling.
28176356	4	56	dep	Fourier	724:730	arg1	transform					732:740	transform	732:740	transform	732:740	The objective of this study is to characterize the adult bovine ACL-to-bone interface using Fourier transform infrared spectroscopic imaging (FTIRI), testing the hypothesis that regional changes in collagen, proteoglycan, and mineral distribution, as well as matrix organization, persist at the mature insertion.
28176356	7	57	from	role	1409:1412	arg1	remodeling					1457:1466	postnatal matrix remodeling	1440:1466	postnatal matrix remodeling	1440:1466	These observations reveal significant changes in collagen distribution and alignment with maturity, and these trends underscore the role of physiologic loading in postnatal matrix remodeling.
28176356	4	58	theme	study	654:658	arg1	objective					636:644	The objective	632:644	The objective of this study	632:658	The objective of this study is to characterize the adult bovine ACL-to-bone interface using Fourier transform infrared spectroscopic imaging (FTIRI), testing the hypothesis that regional changes in collagen, proteoglycan, and mineral distribution, as well as matrix organization, persist at the mature insertion.
28176356	4	59	dep	transform	732:740	arg1	infrared					742:749	infrared	742:749	infrared spectroscopic imaging (FTIRI)	742:779	The objective of this study is to characterize the adult bovine ACL-to-bone interface using Fourier transform infrared spectroscopic imaging (FTIRI), testing the hypothesis that regional changes in collagen, proteoglycan, and mineral distribution, as well as matrix organization, persist at the mature insertion.
28176356	5	60	theme	collagen	1040:1047	arg1	alignment					1049:1057	collagen alignment	1040:1057	collagen alignment	1040:1057	It was observed that while collagen content increases continuously across the adult interface, collagen alignment decreases between ligament and bone.
28176356	3	61	theme	matrix	525:530	arg1	composition					532:542	matrix composition	525:542	matrix composition	525:542	Presently, there is a lack of quantitative understanding of the matrix composition and organization across this junction, especially after the onset of skeletal maturity.
28176356	1	62	theme	mineralized	249:259	arg1	fibrocartilage					261:274	mineralized fibrocartilage	249:274	mineralized fibrocartilage	249:274	The anterior cruciate ligament (ACL)-to-bone interface constitutes a complex, multi-tissue structure comprised of contiguous ligament, non-mineralized fibrocartilage, mineralized fibrocartilage, and bone regions.
28176356	4	63	theme	adult	683:687	arg1	interface					708:716	the adult bovine ACL-to-bone interface	679:716	the adult bovine ACL-to-bone interface using Fourier transform infrared spectroscopic imaging (FTIRI), testing the hypothesis that regional changes in collagen, proteoglycan, and mineral distribution, as well as matrix organization, persist at the mature insertion	679:942	The objective of this study is to characterize the adult bovine ACL-to-bone interface using Fourier transform infrared spectroscopic imaging (FTIRI), testing the hypothesis that regional changes in collagen, proteoglycan, and mineral distribution, as well as matrix organization, persist at the mature insertion.
28176356	7	64	theme	postnatal	1440:1448	arg1	remodeling					1457:1466	postnatal matrix remodeling	1440:1466	postnatal matrix remodeling	1440:1466	These observations reveal significant changes in collagen distribution and alignment with maturity, and these trends underscore the role of physiologic loading in postnatal matrix remodeling.
28176356	6	65	theme	exponential	1171:1181	arg1	increase					1183:1190	an exponential increase	1168:1190	an exponential increase in mineral content	1168:1209	Proteoglycans were primarily localized to the fibrocartilage region and an exponential increase in mineral content was observed between the non-mineralized and mineralized regions.
28176356	7	66	theme	collagen	1326:1333	arg1	distribution					1335:1346	collagen distribution	1326:1346	collagen distribution	1326:1346	These observations reveal significant changes in collagen distribution and alignment with maturity, and these trends underscore the role of physiologic loading in postnatal matrix remodeling.
28176356	8	67	theme	soft	1625:1628	arg1	repair					1637:1642	integrative soft tissue repair	1613:1642	integrative soft tissue repair	1613:1642	Findings from this study provide new insights into interface organization and serve as benchmark design criteria for interface regeneration and integrative soft tissue repair.
28176356	7	68	theme	matrix	1450:1455	arg1	remodeling					1457:1466	postnatal matrix remodeling	1440:1466	postnatal matrix remodeling	1440:1466	These observations reveal significant changes in collagen distribution and alignment with maturity, and these trends underscore the role of physiologic loading in postnatal matrix remodeling.
28176356	1	69	theme	complex	151:157	arg1	structure					173:181	a complex, multi-tissue structure	149:181	a complex, multi-tissue structure comprised of contiguous ligament, non-mineralized fibrocartilage, mineralized fibrocartilage, and bone regions	149:292	The anterior cruciate ligament (ACL)-to-bone interface constitutes a complex, multi-tissue structure comprised of contiguous ligament, non-mineralized fibrocartilage, mineralized fibrocartilage, and bone regions.
28176356	8	70	from	study	1488:1492	arg1	criteria					1573:1580	benchmark design criteria	1556:1580	benchmark design criteria for interface regeneration and integrative soft tissue repair	1556:1642	Findings from this study provide new insights into interface organization and serve as benchmark design criteria for interface regeneration and integrative soft tissue repair.
28176356	8	70	from	study	1488:1492	arg1	Findings					1469:1476	Findings	1469:1476	Findings from this study	1469:1492	Findings from this study provide new insights into interface organization and serve as benchmark design criteria for interface regeneration and integrative soft tissue repair.
28554645	4	0	theme	bands	705:709	arg1	shift					683:687	the shift	679:687	the shift of the amide-II bands of polymers	679:721	Both hydrogen bonds and electrostatic interactions are formed between gelatin and chitosan, as evidenced by the shift of the amide-II bands of polymers.
28554645	6	1	located	observed	893:900	arg2	Tg					885:886	Tg	885:886	Tg	885:886	Only one glass transition temperature (Tg) was observed in composite films with different composition ratios, and it decreases gradually with an increase in chitosan proportion, indicating that gelatin and chitosan have good miscibility and form a wide range of blends.
28554645	6	1	located	observed	893:900	arg1	films					915:919	composite films	905:919	composite films with different composition ratios	905:953	Only one glass transition temperature (Tg) was observed in composite films with different composition ratios, and it decreases gradually with an increase in chitosan proportion, indicating that gelatin and chitosan have good miscibility and form a wide range of blends.
28554645	6	1	located	observed	893:900	arg2	temperature					872:882	Only one glass transition temperature	846:882	Only one glass transition temperature (Tg)	846:887	Only one glass transition temperature (Tg) was observed in composite films with different composition ratios, and it decreases gradually with an increase in chitosan proportion, indicating that gelatin and chitosan have good miscibility and form a wide range of blends.
28554645	1	2	theme	rheological	144:154	arg1	properties					156:165	the rheological properties	140:165	the rheological properties	140:165	Gelatin and chitosan were mixed at different mass ratios in solution forms, and the rheological properties of these film-forming solutions, upon cooling, were studied.
28554645	6	3	theme	chitosan	1003:1010	arg1	proportion					1012:1021	chitosan proportion	1003:1021	chitosan proportion	1003:1021	Only one glass transition temperature (Tg) was observed in composite films with different composition ratios, and it decreases gradually with an increase in chitosan proportion, indicating that gelatin and chitosan have good miscibility and form a wide range of blends.
28554645	0	4	from	interactions	10:21	arg1	films					53:57	gelatin/chitosan composite films	26:57	gelatin/chitosan composite films	26:57	Molecular interactions in gelatin/chitosan composite films.
28554645	6	5	theme	wide	1094:1097	arg1	range					1099:1103	a wide range	1092:1103	a wide range of blends	1092:1113	Only one glass transition temperature (Tg) was observed in composite films with different composition ratios, and it decreases gradually with an increase in chitosan proportion, indicating that gelatin and chitosan have good miscibility and form a wide range of blends.
28554645	6	6	theme	composite	905:913	arg1	films					915:919	composite films	905:919	composite films with different composition ratios	905:953	Only one glass transition temperature (Tg) was observed in composite films with different composition ratios, and it decreases gradually with an increase in chitosan proportion, indicating that gelatin and chitosan have good miscibility and form a wide range of blends.
28554645	3	7	theme	gels	565:568	arg1	modulus					546:552	the storage modulus	534:552	the storage modulus of polymer gels	534:568	Furthermore, these molecular interactions hinder the formation of gelatin networks, consequently decreasing the storage modulus of polymer gels.
28554645	3	8	theme	polymer	557:563	arg1	gels					565:568	polymer gels	557:568	polymer gels	557:568	Furthermore, these molecular interactions hinder the formation of gelatin networks, consequently decreasing the storage modulus of polymer gels.
28554645	4	9	theme	electrostatic	595:607	arg1	interactions					609:620	electrostatic interactions	595:620	electrostatic interactions	595:620	Both hydrogen bonds and electrostatic interactions are formed between gelatin and chitosan, as evidenced by the shift of the amide-II bands of polymers.
28554645	5	10	theme	composite	742:750	arg1	films					752:756	composite films	742:756	composite films	742:756	X-ray patterns of composite films indicate that the contents of triple helices decrease with increasing chitosan content.
28554645	3	11	theme	gelatin	492:498	arg1	networks					500:507	gelatin networks	492:507	gelatin networks	492:507	Furthermore, these molecular interactions hinder the formation of gelatin networks, consequently decreasing the storage modulus of polymer gels.
28554645	6	12	from	increase	991:998	arg1	proportion					1012:1021	chitosan proportion	1003:1021	chitosan proportion	1003:1021	Only one glass transition temperature (Tg) was observed in composite films with different composition ratios, and it decreases gradually with an increase in chitosan proportion, indicating that gelatin and chitosan have good miscibility and form a wide range of blends.
28554645	3	13	theme	storage	538:544	arg1	modulus					546:552	the storage modulus	534:552	the storage modulus of polymer gels	534:568	Furthermore, these molecular interactions hinder the formation of gelatin networks, consequently decreasing the storage modulus of polymer gels.
28554645	2	14	theme	storage	389:395	arg1	modulus					397:403	the storage modulus	385:403	the storage modulus of gelatin solution	385:423	The results indicate that the significant interactions between gelatin and chitosan promote the formation of multiple complexes, reflected by an increase in the storage modulus of gelatin solution.
28554645	5	15	theme	films	752:756	arg1	patterns					730:737	X-ray patterns	724:737	X-ray patterns of composite films	724:756	X-ray patterns of composite films indicate that the contents of triple helices decrease with increasing chitosan content.
28554645	2	16	theme	significant	258:268	arg1	interactions					270:281	the significant interactions	254:281	the significant interactions between gelatin and chitosan	254:310	The results indicate that the significant interactions between gelatin and chitosan promote the formation of multiple complexes, reflected by an increase in the storage modulus of gelatin solution.
28554645	6	17	contain	have	1061:1064	arg1	chitosan					1052:1059	chitosan	1052:1059	chitosan	1052:1059	Only one glass transition temperature (Tg) was observed in composite films with different composition ratios, and it decreases gradually with an increase in chitosan proportion, indicating that gelatin and chitosan have good miscibility and form a wide range of blends.
28554645	6	17	contain	have	1061:1064	arg1	gelatin					1040:1046	gelatin	1040:1046	gelatin	1040:1046	Only one glass transition temperature (Tg) was observed in composite films with different composition ratios, and it decreases gradually with an increase in chitosan proportion, indicating that gelatin and chitosan have good miscibility and form a wide range of blends.
28554645	6	17	contain	have	1061:1064	arg2	miscibility					1071:1081	good miscibility	1066:1081	good miscibility	1066:1081	Only one glass transition temperature (Tg) was observed in composite films with different composition ratios, and it decreases gradually with an increase in chitosan proportion, indicating that gelatin and chitosan have good miscibility and form a wide range of blends.
28554645	1	18	theme	film-forming	176:187	arg1	solutions					189:197	these film-forming solutions	170:197	these film-forming solutions	170:197	Gelatin and chitosan were mixed at different mass ratios in solution forms, and the rheological properties of these film-forming solutions, upon cooling, were studied.
28554645	0	19	theme	Molecular	0:8	arg1	interactions					10:21	Molecular interactions	0:21	Molecular interactions in gelatin/chitosan composite films	0:57	Molecular interactions in gelatin/chitosan composite films.
28554645	1	20	theme	different	95:103	arg1	ratios					110:115	different mass ratios	95:115	different mass ratios in solution forms	95:133	Gelatin and chitosan were mixed at different mass ratios in solution forms, and the rheological properties of these film-forming solutions, upon cooling, were studied.
28554645	1	21	theme	solutions	189:197	arg1	properties					156:165	the rheological properties	140:165	the rheological properties	140:165	Gelatin and chitosan were mixed at different mass ratios in solution forms, and the rheological properties of these film-forming solutions, upon cooling, were studied.
28554645	0	22	theme	gelatin/chitosan	26:41	arg1	films					53:57	gelatin/chitosan composite films	26:57	gelatin/chitosan composite films	26:57	Molecular interactions in gelatin/chitosan composite films.
28554645	1	23	theme	mass	105:108	arg1	ratios					110:115	different mass ratios	95:115	different mass ratios in solution forms	95:133	Gelatin and chitosan were mixed at different mass ratios in solution forms, and the rheological properties of these film-forming solutions, upon cooling, were studied.
28554645	2	24	theme	solution	416:423	arg1	modulus					397:403	the storage modulus	385:403	the storage modulus of gelatin solution	385:423	The results indicate that the significant interactions between gelatin and chitosan promote the formation of multiple complexes, reflected by an increase in the storage modulus of gelatin solution.
28554645	6	25	theme	blends	1108:1113	arg1	range					1099:1103	a wide range	1092:1103	a wide range of blends	1092:1113	Only one glass transition temperature (Tg) was observed in composite films with different composition ratios, and it decreases gradually with an increase in chitosan proportion, indicating that gelatin and chitosan have good miscibility and form a wide range of blends.
28554645	4	26	theme	hydrogen	576:583	arg1	bonds					585:589	hydrogen bonds	576:589	hydrogen bonds	576:589	Both hydrogen bonds and electrostatic interactions are formed between gelatin and chitosan, as evidenced by the shift of the amide-II bands of polymers.
28554645	3	27	theme	networks	500:507	arg1	formation					479:487	the formation	475:487	the formation of gelatin networks	475:507	Furthermore, these molecular interactions hinder the formation of gelatin networks, consequently decreasing the storage modulus of polymer gels.
28554645	2	28	theme	gelatin	408:414	arg1	solution					416:423	gelatin solution	408:423	gelatin solution	408:423	The results indicate that the significant interactions between gelatin and chitosan promote the formation of multiple complexes, reflected by an increase in the storage modulus of gelatin solution.
28554645	2	29	theme	multiple	337:344	arg1	complexes					346:354	multiple complexes	337:354	multiple complexes	337:354	The results indicate that the significant interactions between gelatin and chitosan promote the formation of multiple complexes, reflected by an increase in the storage modulus of gelatin solution.
28554645	6	30	theme	good	1066:1069	arg1	miscibility					1071:1081	good miscibility	1066:1081	good miscibility	1066:1081	Only one glass transition temperature (Tg) was observed in composite films with different composition ratios, and it decreases gradually with an increase in chitosan proportion, indicating that gelatin and chitosan have good miscibility and form a wide range of blends.
28554645	3	31	theme	molecular	445:453	arg1	interactions					455:466	these molecular interactions	439:466	these molecular interactions	439:466	Furthermore, these molecular interactions hinder the formation of gelatin networks, consequently decreasing the storage modulus of polymer gels.
28554645	0	32	theme	composite	43:51	arg1	films					53:57	gelatin/chitosan composite films	26:57	gelatin/chitosan composite films	26:57	Molecular interactions in gelatin/chitosan composite films.
28554645	5	33	theme	X-ray	724:728	arg1	patterns					730:737	X-ray patterns	724:737	X-ray patterns of composite films	724:756	X-ray patterns of composite films indicate that the contents of triple helices decrease with increasing chitosan content.
28554645	6	34	with	films	915:919	arg1	ratios					948:953	different composition ratios	926:953	different composition ratios	926:953	Only one glass transition temperature (Tg) was observed in composite films with different composition ratios, and it decreases gradually with an increase in chitosan proportion, indicating that gelatin and chitosan have good miscibility and form a wide range of blends.
28554645	5	35	theme	chitosan	828:835	arg1	content					837:843	chitosan content	828:843	chitosan content	828:843	X-ray patterns of composite films indicate that the contents of triple helices decrease with increasing chitosan content.
28554645	5	36	theme	helices	795:801	arg1	contents					776:783	the contents	772:783	the contents of triple helices	772:801	X-ray patterns of composite films indicate that the contents of triple helices decrease with increasing chitosan content.
28554645	6	37	theme	transition	861:870	arg1	temperature					872:882	Only one glass transition temperature	846:882	Only one glass transition temperature (Tg)	846:887	Only one glass transition temperature (Tg) was observed in composite films with different composition ratios, and it decreases gradually with an increase in chitosan proportion, indicating that gelatin and chitosan have good miscibility and form a wide range of blends.
28554645	6	37	theme	transition	861:870	arg1	Tg					885:886	Tg	885:886	Tg	885:886	Only one glass transition temperature (Tg) was observed in composite films with different composition ratios, and it decreases gradually with an increase in chitosan proportion, indicating that gelatin and chitosan have good miscibility and form a wide range of blends.
28554645	2	38	from	increase	373:380	arg1	modulus					397:403	the storage modulus	385:403	the storage modulus of gelatin solution	385:423	The results indicate that the significant interactions between gelatin and chitosan promote the formation of multiple complexes, reflected by an increase in the storage modulus of gelatin solution.
28554645	2	39	theme	complexes	346:354	arg1	formation					324:332	the formation	320:332	the formation of multiple complexes	320:354	The results indicate that the significant interactions between gelatin and chitosan promote the formation of multiple complexes, reflected by an increase in the storage modulus of gelatin solution.
28554645	6	40	theme	glass	855:859	arg1	temperature					872:882	Only one glass transition temperature	846:882	Only one glass transition temperature (Tg)	846:887	Only one glass transition temperature (Tg) was observed in composite films with different composition ratios, and it decreases gradually with an increase in chitosan proportion, indicating that gelatin and chitosan have good miscibility and form a wide range of blends.
28554645	6	40	theme	glass	855:859	arg1	Tg					885:886	Tg	885:886	Tg	885:886	Only one glass transition temperature (Tg) was observed in composite films with different composition ratios, and it decreases gradually with an increase in chitosan proportion, indicating that gelatin and chitosan have good miscibility and form a wide range of blends.
28554645	1	41	theme	solution	120:127	arg1	forms					129:133	solution forms	120:133	solution forms	120:133	Gelatin and chitosan were mixed at different mass ratios in solution forms, and the rheological properties of these film-forming solutions, upon cooling, were studied.
28554645	6	42	theme	composition	936:946	arg1	ratios					948:953	different composition ratios	926:953	different composition ratios	926:953	Only one glass transition temperature (Tg) was observed in composite films with different composition ratios, and it decreases gradually with an increase in chitosan proportion, indicating that gelatin and chitosan have good miscibility and form a wide range of blends.
28554645	4	43	theme	polymers	714:721	arg1	bands					705:709	the amide-II bands	692:709	the amide-II bands of polymers	692:721	Both hydrogen bonds and electrostatic interactions are formed between gelatin and chitosan, as evidenced by the shift of the amide-II bands of polymers.
28554645	1	44	from	ratios	110:115	arg1	forms					129:133	solution forms	120:133	solution forms	120:133	Gelatin and chitosan were mixed at different mass ratios in solution forms, and the rheological properties of these film-forming solutions, upon cooling, were studied.
28554645	2	45	dep	promote	312:318	arg1	reflected					357:365	reflected	357:365	reflected by an increase in the storage modulus of gelatin solution	357:423	The results indicate that the significant interactions between gelatin and chitosan promote the formation of multiple complexes, reflected by an increase in the storage modulus of gelatin solution.
28554645	6	46	theme	different	926:934	arg1	ratios					948:953	different composition ratios	926:953	different composition ratios	926:953	Only one glass transition temperature (Tg) was observed in composite films with different composition ratios, and it decreases gradually with an increase in chitosan proportion, indicating that gelatin and chitosan have good miscibility and form a wide range of blends.
28554645	5	47	theme	triple	788:793	arg1	helices					795:801	triple helices	788:801	triple helices	788:801	X-ray patterns of composite films indicate that the contents of triple helices decrease with increasing chitosan content.
24978042	1	0	theme	-glucan	379:385	arg1	ends					400:403	α-(1 → 4)-glucan non-reducing ends	370:403	α-(1 → 4)-glucan non-reducing ends	370:403	This article reports the enzymatic synthesis of dendritic amphoteric α-glucans having both glucuronic acid and glucosamine residues at the non-reducing ends by thermostable phosphorylase-catalyzed successive glucuronylation and glucosaminylation of a glucan dendrimer having α-(1 → 4)-glucan non-reducing ends using α-D-glucuronic acid 1-phosphate and α-D-glucosamine 1-phosphate as glycosyl donors, respectively.
24978042	1	1	theme	glucuronic	186:195	arg1	acid					197:200	both glucuronic acid and glucosamine residues	181:225	acid	197:200	This article reports the enzymatic synthesis of dendritic amphoteric α-glucans having both glucuronic acid and glucosamine residues at the non-reducing ends by thermostable phosphorylase-catalyzed successive glucuronylation and glucosaminylation of a glucan dendrimer having α-(1 → 4)-glucan non-reducing ends using α-D-glucuronic acid 1-phosphate and α-D-glucosamine 1-phosphate as glycosyl donors, respectively.
24978042	4	2	from	pI	723:724	arg1	water					709:713	water	709:713	water at pH = pI to form large aggregates	709:749	These materials self-assemble in water at pH = pI to form large aggregates, but disassemble at pH shifted from pI.
24978042	2	3	theme	NMR	564:566	arg1	analysis					568:575	the (1)H NMR analysis	555:575	the (1)H NMR analysis	555:575	The structure of the products is confirmed by the (1)H NMR analysis.
24978042	1	4	theme	non-reducing	387:398	arg1	ends					400:403	α-(1 → 4)-glucan non-reducing ends	370:403	α-(1 → 4)-glucan non-reducing ends	370:403	This article reports the enzymatic synthesis of dendritic amphoteric α-glucans having both glucuronic acid and glucosamine residues at the non-reducing ends by thermostable phosphorylase-catalyzed successive glucuronylation and glucosaminylation of a glucan dendrimer having α-(1 → 4)-glucan non-reducing ends using α-D-glucuronic acid 1-phosphate and α-D-glucosamine 1-phosphate as glycosyl donors, respectively.
24978042	1	5	contain	having	363:368	arg1	dendrimer					353:361	a glucan dendrimer	344:361	a glucan dendrimer having α-(1 → 4)-glucan non-reducing ends	344:403	This article reports the enzymatic synthesis of dendritic amphoteric α-glucans having both glucuronic acid and glucosamine residues at the non-reducing ends by thermostable phosphorylase-catalyzed successive glucuronylation and glucosaminylation of a glucan dendrimer having α-(1 → 4)-glucan non-reducing ends using α-D-glucuronic acid 1-phosphate and α-D-glucosamine 1-phosphate as glycosyl donors, respectively.
24978042	1	5	contain	having	363:368	arg2	ends					400:403	α-(1 → 4)-glucan non-reducing ends	370:403	α-(1 → 4)-glucan non-reducing ends	370:403	This article reports the enzymatic synthesis of dendritic amphoteric α-glucans having both glucuronic acid and glucosamine residues at the non-reducing ends by thermostable phosphorylase-catalyzed successive glucuronylation and glucosaminylation of a glucan dendrimer having α-(1 → 4)-glucan non-reducing ends using α-D-glucuronic acid 1-phosphate and α-D-glucosamine 1-phosphate as glycosyl donors, respectively.
24978042	3	6	theme	inherent	599:606	arg1	pIs					628:630	pIs	628:630	pIs	628:630	The products exhibit inherent isoelectric points (pIs) determined by the ζ-potential measurement.
24978042	3	6	theme	inherent	599:606	arg1	points					620:625	inherent isoelectric points	599:625	inherent isoelectric points (pIs) determined by the ζ-potential measurement	599:673	The products exhibit inherent isoelectric points (pIs) determined by the ζ-potential measurement.
24978042	2	7	theme	H	562:562	arg1	analysis					568:575	the (1)H NMR analysis	555:575	the (1)H NMR analysis	555:575	The structure of the products is confirmed by the (1)H NMR analysis.
24978042	1	8	contain	having	174:179	arg2	acid					197:200	both glucuronic acid and glucosamine residues	181:225	acid	197:200	This article reports the enzymatic synthesis of dendritic amphoteric α-glucans having both glucuronic acid and glucosamine residues at the non-reducing ends by thermostable phosphorylase-catalyzed successive glucuronylation and glucosaminylation of a glucan dendrimer having α-(1 → 4)-glucan non-reducing ends using α-D-glucuronic acid 1-phosphate and α-D-glucosamine 1-phosphate as glycosyl donors, respectively.
24978042	1	8	contain	having	174:179	arg2	residues					218:225	both glucuronic acid and glucosamine residues	181:225	residues	218:225	This article reports the enzymatic synthesis of dendritic amphoteric α-glucans having both glucuronic acid and glucosamine residues at the non-reducing ends by thermostable phosphorylase-catalyzed successive glucuronylation and glucosaminylation of a glucan dendrimer having α-(1 → 4)-glucan non-reducing ends using α-D-glucuronic acid 1-phosphate and α-D-glucosamine 1-phosphate as glycosyl donors, respectively.
24978042	1	8	contain	having	174:179	arg1	α-glucans					164:172	dendritic amphoteric α-glucans	143:172	dendritic amphoteric α-glucans having both glucuronic acid and glucosamine residues at the non-reducing ends	143:250	This article reports the enzymatic synthesis of dendritic amphoteric α-glucans having both glucuronic acid and glucosamine residues at the non-reducing ends by thermostable phosphorylase-catalyzed successive glucuronylation and glucosaminylation of a glucan dendrimer having α-(1 → 4)-glucan non-reducing ends using α-D-glucuronic acid 1-phosphate and α-D-glucosamine 1-phosphate as glycosyl donors, respectively.
24978042	1	9	theme	acid	426:429	arg1	1-phosphate					431:441	α-D-glucuronic acid 1-phosphate	411:441	α-D-glucuronic acid 1-phosphate	411:441	This article reports the enzymatic synthesis of dendritic amphoteric α-glucans having both glucuronic acid and glucosamine residues at the non-reducing ends by thermostable phosphorylase-catalyzed successive glucuronylation and glucosaminylation of a glucan dendrimer having α-(1 → 4)-glucan non-reducing ends using α-D-glucuronic acid 1-phosphate and α-D-glucosamine 1-phosphate as glycosyl donors, respectively.
24978042	1	9	theme	acid	426:429	arg1	1-phosphate					463:473	α-D-glucosamine 1-phosphate	447:473	α-D-glucosamine 1-phosphate	447:473	This article reports the enzymatic synthesis of dendritic amphoteric α-glucans having both glucuronic acid and glucosamine residues at the non-reducing ends by thermostable phosphorylase-catalyzed successive glucuronylation and glucosaminylation of a glucan dendrimer having α-(1 → 4)-glucan non-reducing ends using α-D-glucuronic acid 1-phosphate and α-D-glucosamine 1-phosphate as glycosyl donors, respectively.
24978042	1	9	theme	acid	426:429	arg1	donors					487:492	glycosyl donors	478:492	glycosyl donors	478:492	This article reports the enzymatic synthesis of dendritic amphoteric α-glucans having both glucuronic acid and glucosamine residues at the non-reducing ends by thermostable phosphorylase-catalyzed successive glucuronylation and glucosaminylation of a glucan dendrimer having α-(1 → 4)-glucan non-reducing ends using α-D-glucuronic acid 1-phosphate and α-D-glucosamine 1-phosphate as glycosyl donors, respectively.
24978042	3	10	theme	isoelectric	608:618	arg1	pIs					628:630	pIs	628:630	pIs	628:630	The products exhibit inherent isoelectric points (pIs) determined by the ζ-potential measurement.
24978042	3	10	theme	isoelectric	608:618	arg1	points					620:625	inherent isoelectric points	599:625	inherent isoelectric points (pIs) determined by the ζ-potential measurement	599:673	The products exhibit inherent isoelectric points (pIs) determined by the ζ-potential measurement.
24978042	1	11	theme	glucosamine	206:216	arg1	residues					218:225	both glucuronic acid and glucosamine residues	181:225	residues	218:225	This article reports the enzymatic synthesis of dendritic amphoteric α-glucans having both glucuronic acid and glucosamine residues at the non-reducing ends by thermostable phosphorylase-catalyzed successive glucuronylation and glucosaminylation of a glucan dendrimer having α-(1 → 4)-glucan non-reducing ends using α-D-glucuronic acid 1-phosphate and α-D-glucosamine 1-phosphate as glycosyl donors, respectively.
24978042	0	12	theme	Enzymatic	0:8	arg1	synthesis					10:18	Enzymatic synthesis	0:18	Enzymatic synthesis of dendritic amphoteric	0:42	Enzymatic synthesis of dendritic amphoteric α-glucans by thermostable phosphorylase catalysis.
24978042	0	13	theme	dendritic	23:31	arg1	amphoteric					33:42	dendritic amphoteric	23:42	dendritic amphoteric	23:42	Enzymatic synthesis of dendritic amphoteric α-glucans by thermostable phosphorylase catalysis.
24978042	1	14	theme	glucan	346:351	arg1	dendrimer					353:361	a glucan dendrimer	344:361	a glucan dendrimer having α-(1 → 4)-glucan non-reducing ends	344:403	This article reports the enzymatic synthesis of dendritic amphoteric α-glucans having both glucuronic acid and glucosamine residues at the non-reducing ends by thermostable phosphorylase-catalyzed successive glucuronylation and glucosaminylation of a glucan dendrimer having α-(1 → 4)-glucan non-reducing ends using α-D-glucuronic acid 1-phosphate and α-D-glucosamine 1-phosphate as glycosyl donors, respectively.
24978042	1	15	theme	dendrimer	353:361	arg1	glucosaminylation					323:339	glucosaminylation	323:339	glucosaminylation	323:339	This article reports the enzymatic synthesis of dendritic amphoteric α-glucans having both glucuronic acid and glucosamine residues at the non-reducing ends by thermostable phosphorylase-catalyzed successive glucuronylation and glucosaminylation of a glucan dendrimer having α-(1 → 4)-glucan non-reducing ends using α-D-glucuronic acid 1-phosphate and α-D-glucosamine 1-phosphate as glycosyl donors, respectively.
24978042	1	15	theme	dendrimer	353:361	arg1	glucuronylation					303:317	thermostable phosphorylase-catalyzed successive glucuronylation	255:317	thermostable phosphorylase-catalyzed successive glucuronylation	255:317	This article reports the enzymatic synthesis of dendritic amphoteric α-glucans having both glucuronic acid and glucosamine residues at the non-reducing ends by thermostable phosphorylase-catalyzed successive glucuronylation and glucosaminylation of a glucan dendrimer having α-(1 → 4)-glucan non-reducing ends using α-D-glucuronic acid 1-phosphate and α-D-glucosamine 1-phosphate as glycosyl donors, respectively.
24978042	1	16	theme	enzymatic	120:128	arg1	synthesis					130:138	the enzymatic synthesis	116:138	the enzymatic synthesis of dendritic amphoteric α-glucans having both glucuronic acid and glucosamine residues at the non-reducing ends	116:250	This article reports the enzymatic synthesis of dendritic amphoteric α-glucans having both glucuronic acid and glucosamine residues at the non-reducing ends by thermostable phosphorylase-catalyzed successive glucuronylation and glucosaminylation of a glucan dendrimer having α-(1 → 4)-glucan non-reducing ends using α-D-glucuronic acid 1-phosphate and α-D-glucosamine 1-phosphate as glycosyl donors, respectively.
24978042	1	17	dep	α-	370:371	arg1	4					377:377	4	377:377	4	377:377	This article reports the enzymatic synthesis of dendritic amphoteric α-glucans having both glucuronic acid and glucosamine residues at the non-reducing ends by thermostable phosphorylase-catalyzed successive glucuronylation and glucosaminylation of a glucan dendrimer having α-(1 → 4)-glucan non-reducing ends using α-D-glucuronic acid 1-phosphate and α-D-glucosamine 1-phosphate as glycosyl donors, respectively.
24978042	1	17	dep	α-	370:371	arg1	1					373:373	1	373:373	1	373:373	This article reports the enzymatic synthesis of dendritic amphoteric α-glucans having both glucuronic acid and glucosamine residues at the non-reducing ends by thermostable phosphorylase-catalyzed successive glucuronylation and glucosaminylation of a glucan dendrimer having α-(1 → 4)-glucan non-reducing ends using α-D-glucuronic acid 1-phosphate and α-D-glucosamine 1-phosphate as glycosyl donors, respectively.
24978042	1	18	theme	non-reducing	234:245	arg1	ends					247:250	the non-reducing ends	230:250	the non-reducing ends	230:250	This article reports the enzymatic synthesis of dendritic amphoteric α-glucans having both glucuronic acid and glucosamine residues at the non-reducing ends by thermostable phosphorylase-catalyzed successive glucuronylation and glucosaminylation of a glucan dendrimer having α-(1 → 4)-glucan non-reducing ends using α-D-glucuronic acid 1-phosphate and α-D-glucosamine 1-phosphate as glycosyl donors, respectively.
24978042	0	19	theme	amphoteric	33:42	arg1	synthesis					10:18	Enzymatic synthesis	0:18	Enzymatic synthesis of dendritic amphoteric	0:42	Enzymatic synthesis of dendritic amphoteric α-glucans by thermostable phosphorylase catalysis.
24978042	1	20	theme	α-	370:371	arg1	ends					400:403	α-(1 → 4)-glucan non-reducing ends	370:403	α-(1 → 4)-glucan non-reducing ends	370:403	This article reports the enzymatic synthesis of dendritic amphoteric α-glucans having both glucuronic acid and glucosamine residues at the non-reducing ends by thermostable phosphorylase-catalyzed successive glucuronylation and glucosaminylation of a glucan dendrimer having α-(1 → 4)-glucan non-reducing ends using α-D-glucuronic acid 1-phosphate and α-D-glucosamine 1-phosphate as glycosyl donors, respectively.
24978042	2	21	theme	products	530:537	arg1	structure					513:521	The structure	509:521	The structure of the products	509:537	The structure of the products is confirmed by the (1)H NMR analysis.
24978042	1	22	theme	α-D-glucosamine	447:461	arg1	1-phosphate					431:441	α-D-glucuronic acid 1-phosphate	411:441	α-D-glucuronic acid 1-phosphate	411:441	This article reports the enzymatic synthesis of dendritic amphoteric α-glucans having both glucuronic acid and glucosamine residues at the non-reducing ends by thermostable phosphorylase-catalyzed successive glucuronylation and glucosaminylation of a glucan dendrimer having α-(1 → 4)-glucan non-reducing ends using α-D-glucuronic acid 1-phosphate and α-D-glucosamine 1-phosphate as glycosyl donors, respectively.
24978042	1	22	theme	α-D-glucosamine	447:461	arg1	1-phosphate					463:473	α-D-glucosamine 1-phosphate	447:473	α-D-glucosamine 1-phosphate	447:473	This article reports the enzymatic synthesis of dendritic amphoteric α-glucans having both glucuronic acid and glucosamine residues at the non-reducing ends by thermostable phosphorylase-catalyzed successive glucuronylation and glucosaminylation of a glucan dendrimer having α-(1 → 4)-glucan non-reducing ends using α-D-glucuronic acid 1-phosphate and α-D-glucosamine 1-phosphate as glycosyl donors, respectively.
24978042	1	22	theme	α-D-glucosamine	447:461	arg1	donors					487:492	glycosyl donors	478:492	glycosyl donors	478:492	This article reports the enzymatic synthesis of dendritic amphoteric α-glucans having both glucuronic acid and glucosamine residues at the non-reducing ends by thermostable phosphorylase-catalyzed successive glucuronylation and glucosaminylation of a glucan dendrimer having α-(1 → 4)-glucan non-reducing ends using α-D-glucuronic acid 1-phosphate and α-D-glucosamine 1-phosphate as glycosyl donors, respectively.
24978042	0	23	theme	thermostable	57:68	arg1	catalysis					84:92	thermostable phosphorylase catalysis	57:92	thermostable phosphorylase catalysis	57:92	Enzymatic synthesis of dendritic amphoteric α-glucans by thermostable phosphorylase catalysis.
24978042	1	24	theme	α-D-glucuronic	411:424	arg1	1-phosphate					431:441	α-D-glucuronic acid 1-phosphate	411:441	α-D-glucuronic acid 1-phosphate	411:441	This article reports the enzymatic synthesis of dendritic amphoteric α-glucans having both glucuronic acid and glucosamine residues at the non-reducing ends by thermostable phosphorylase-catalyzed successive glucuronylation and glucosaminylation of a glucan dendrimer having α-(1 → 4)-glucan non-reducing ends using α-D-glucuronic acid 1-phosphate and α-D-glucosamine 1-phosphate as glycosyl donors, respectively.
24978042	1	24	theme	α-D-glucuronic	411:424	arg1	1-phosphate					463:473	α-D-glucosamine 1-phosphate	447:473	α-D-glucosamine 1-phosphate	447:473	This article reports the enzymatic synthesis of dendritic amphoteric α-glucans having both glucuronic acid and glucosamine residues at the non-reducing ends by thermostable phosphorylase-catalyzed successive glucuronylation and glucosaminylation of a glucan dendrimer having α-(1 → 4)-glucan non-reducing ends using α-D-glucuronic acid 1-phosphate and α-D-glucosamine 1-phosphate as glycosyl donors, respectively.
24978042	1	24	theme	α-D-glucuronic	411:424	arg1	donors					487:492	glycosyl donors	478:492	glycosyl donors	478:492	This article reports the enzymatic synthesis of dendritic amphoteric α-glucans having both glucuronic acid and glucosamine residues at the non-reducing ends by thermostable phosphorylase-catalyzed successive glucuronylation and glucosaminylation of a glucan dendrimer having α-(1 → 4)-glucan non-reducing ends using α-D-glucuronic acid 1-phosphate and α-D-glucosamine 1-phosphate as glycosyl donors, respectively.
24978042	4	25	theme	large	734:738	arg1	aggregates					740:749	large aggregates	734:749	large aggregates	734:749	These materials self-assemble in water at pH = pI to form large aggregates, but disassemble at pH shifted from pI.
24978042	1	26	theme	glycosyl	478:485	arg1	1-phosphate					431:441	α-D-glucuronic acid 1-phosphate	411:441	α-D-glucuronic acid 1-phosphate	411:441	This article reports the enzymatic synthesis of dendritic amphoteric α-glucans having both glucuronic acid and glucosamine residues at the non-reducing ends by thermostable phosphorylase-catalyzed successive glucuronylation and glucosaminylation of a glucan dendrimer having α-(1 → 4)-glucan non-reducing ends using α-D-glucuronic acid 1-phosphate and α-D-glucosamine 1-phosphate as glycosyl donors, respectively.
24978042	1	26	theme	glycosyl	478:485	arg1	1-phosphate					463:473	α-D-glucosamine 1-phosphate	447:473	α-D-glucosamine 1-phosphate	447:473	This article reports the enzymatic synthesis of dendritic amphoteric α-glucans having both glucuronic acid and glucosamine residues at the non-reducing ends by thermostable phosphorylase-catalyzed successive glucuronylation and glucosaminylation of a glucan dendrimer having α-(1 → 4)-glucan non-reducing ends using α-D-glucuronic acid 1-phosphate and α-D-glucosamine 1-phosphate as glycosyl donors, respectively.
24978042	1	26	theme	glycosyl	478:485	arg1	donors					487:492	glycosyl donors	478:492	glycosyl donors	478:492	This article reports the enzymatic synthesis of dendritic amphoteric α-glucans having both glucuronic acid and glucosamine residues at the non-reducing ends by thermostable phosphorylase-catalyzed successive glucuronylation and glucosaminylation of a glucan dendrimer having α-(1 → 4)-glucan non-reducing ends using α-D-glucuronic acid 1-phosphate and α-D-glucosamine 1-phosphate as glycosyl donors, respectively.
24978042	3	27	theme	ζ-potential	651:661	arg1	measurement					663:673	the ζ-potential measurement	647:673	the ζ-potential measurement	647:673	The products exhibit inherent isoelectric points (pIs) determined by the ζ-potential measurement.
24978042	1	28	theme	dendritic	143:151	arg1	α-glucans					164:172	dendritic amphoteric α-glucans	143:172	dendritic amphoteric α-glucans having both glucuronic acid and glucosamine residues at the non-reducing ends	143:250	This article reports the enzymatic synthesis of dendritic amphoteric α-glucans having both glucuronic acid and glucosamine residues at the non-reducing ends by thermostable phosphorylase-catalyzed successive glucuronylation and glucosaminylation of a glucan dendrimer having α-(1 → 4)-glucan non-reducing ends using α-D-glucuronic acid 1-phosphate and α-D-glucosamine 1-phosphate as glycosyl donors, respectively.
24978042	1	29	theme	amphoteric	153:162	arg1	α-glucans					164:172	dendritic amphoteric α-glucans	143:172	dendritic amphoteric α-glucans having both glucuronic acid and glucosamine residues at the non-reducing ends	143:250	This article reports the enzymatic synthesis of dendritic amphoteric α-glucans having both glucuronic acid and glucosamine residues at the non-reducing ends by thermostable phosphorylase-catalyzed successive glucuronylation and glucosaminylation of a glucan dendrimer having α-(1 → 4)-glucan non-reducing ends using α-D-glucuronic acid 1-phosphate and α-D-glucosamine 1-phosphate as glycosyl donors, respectively.
24978042	1	30	theme	thermostable	255:266	arg1	glucuronylation					303:317	thermostable phosphorylase-catalyzed successive glucuronylation	255:317	thermostable phosphorylase-catalyzed successive glucuronylation	255:317	This article reports the enzymatic synthesis of dendritic amphoteric α-glucans having both glucuronic acid and glucosamine residues at the non-reducing ends by thermostable phosphorylase-catalyzed successive glucuronylation and glucosaminylation of a glucan dendrimer having α-(1 → 4)-glucan non-reducing ends using α-D-glucuronic acid 1-phosphate and α-D-glucosamine 1-phosphate as glycosyl donors, respectively.
24978042	1	31	theme	α-glucans	164:172	arg1	synthesis					130:138	the enzymatic synthesis	116:138	the enzymatic synthesis of dendritic amphoteric α-glucans having both glucuronic acid and glucosamine residues at the non-reducing ends	116:250	This article reports the enzymatic synthesis of dendritic amphoteric α-glucans having both glucuronic acid and glucosamine residues at the non-reducing ends by thermostable phosphorylase-catalyzed successive glucuronylation and glucosaminylation of a glucan dendrimer having α-(1 → 4)-glucan non-reducing ends using α-D-glucuronic acid 1-phosphate and α-D-glucosamine 1-phosphate as glycosyl donors, respectively.
24978042	1	32	theme	phosphorylase-catalyzed	268:290	arg1	glucuronylation					303:317	thermostable phosphorylase-catalyzed successive glucuronylation	255:317	thermostable phosphorylase-catalyzed successive glucuronylation	255:317	This article reports the enzymatic synthesis of dendritic amphoteric α-glucans having both glucuronic acid and glucosamine residues at the non-reducing ends by thermostable phosphorylase-catalyzed successive glucuronylation and glucosaminylation of a glucan dendrimer having α-(1 → 4)-glucan non-reducing ends using α-D-glucuronic acid 1-phosphate and α-D-glucosamine 1-phosphate as glycosyl donors, respectively.
24978042	0	33	theme	phosphorylase	70:82	arg1	catalysis					84:92	thermostable phosphorylase catalysis	57:92	thermostable phosphorylase catalysis	57:92	Enzymatic synthesis of dendritic amphoteric α-glucans by thermostable phosphorylase catalysis.
24978042	4	34	theme	=	721:721	arg1	pI					723:724	pH = pI	718:724	pH = pI	718:724	These materials self-assemble in water at pH = pI to form large aggregates, but disassemble at pH shifted from pI.
24978042	1	35	theme	successive	292:301	arg1	glucuronylation					303:317	thermostable phosphorylase-catalyzed successive glucuronylation	255:317	thermostable phosphorylase-catalyzed successive glucuronylation	255:317	This article reports the enzymatic synthesis of dendritic amphoteric α-glucans having both glucuronic acid and glucosamine residues at the non-reducing ends by thermostable phosphorylase-catalyzed successive glucuronylation and glucosaminylation of a glucan dendrimer having α-(1 → 4)-glucan non-reducing ends using α-D-glucuronic acid 1-phosphate and α-D-glucosamine 1-phosphate as glycosyl donors, respectively.
25570974	5	0	theme	cellulose	747:755	arg1	network					757:763	dense cellulose network	741:763	dense cellulose network	741:763	Ultrasonication can disrupt dense cellulose network and produce the long BC/MWCNTs bundles ranging from 30 to 100 μm.
25570974	6	1	dep	carbonyl	905:912	arg1	C=O					915:917	C=O	915:917	C=O	915:917	Raman spectroscopy shows a drop at peak of hydroxyl (-OH, 3700 m(-1)) and carbonyl (C=O, 1600 cm(-1)).
25570974	6	1	dep	carbonyl	905:912	arg1	cm					925:926	1600 cm	920:926	1600 cm(-1)	920:930	Raman spectroscopy shows a drop at peak of hydroxyl (-OH, 3700 m(-1)) and carbonyl (C=O, 1600 cm(-1)).
25570974	7	2	theme	BC	1013:1014	arg1	surface					1016:1022	BC surface	1013:1022	BC surface	1013:1022	This indicates the formation of the continuous MWCNTs-like protection layer on BC surface.
25570974	3	3	theme	bacterial	473:481	arg1	cellulose					483:491	bacterial cellulose	473:491	a long, sensitive and high tensile strength carbon nanotubes (CNT) coated bacterial cellulose (BC) bundle	399:503	In order to overcome the challenges, a novel technique has been developed to produce a long, sensitive and high tensile strength carbon nanotubes (CNT) coated bacterial cellulose (BC) bundle.
25570974	3	3	theme	bacterial	473:481	arg1	BC					494:495	BC	494:495	BC	494:495	In order to overcome the challenges, a novel technique has been developed to produce a long, sensitive and high tensile strength carbon nanotubes (CNT) coated bacterial cellulose (BC) bundle.
25570974	8	4	from	-7	1235:1236	arg1	Sensitivity					1215:1225	80 W. Sensitivity	1209:1225	80 W. Sensitivity from 10(-7) to 10(-9) A of long BC/MWCNTs composite bundles	1209:1285	Electrical properties of the BC/MWCNTs bundles showed linearity from -6 V to +6 V. Composites with BC treated by higher ultrasonic powers, 100 W, show higher conductivity comparing to 80 W. Sensitivity from 10(-7) to 10(-9) A of long BC/MWCNTs composite bundles is reported in this paper.
25570974	4	5	theme	ultrasonication	541:555	arg1	use					534:536	the use	530:536	the use of ultrasonication to perform carboxyl functionalized multi-walled carbon nanotubes (MWCNTs-COOH) self-assembling on the surface of BC bundles (BC/MWCNTs) via hydrogen bonds	530:710	This study demonstrates the use of ultrasonication to perform carboxyl functionalized multi-walled carbon nanotubes (MWCNTs-COOH) self-assembling on the surface of BC bundles (BC/MWCNTs) via hydrogen bonds.
25570974	0	6	theme	potential	75:83	arg1	material					85:92	a potential material	73:92	a potential material for suspended resonator	73:116	MWCNTs-like protection layer formation on bacterial cellulose bundles as a potential material for suspended resonator.
25570974	6	7	theme	hydroxyl	874:881	arg1	peak					866:869	peak	866:869	peak of hydroxyl (-OH, 3700 m(-1)) and carbonyl (C=O, 1600 cm(-1))	866:931	Raman spectroscopy shows a drop at peak of hydroxyl (-OH, 3700 m(-1)) and carbonyl (C=O, 1600 cm(-1)).
25570974	3	8	theme	cellulose	483:491	arg1	bundle					498:503	a long, sensitive and high tensile strength carbon nanotubes (CNT) coated bacterial cellulose (BC) bundle	399:503	a long, sensitive and high tensile strength carbon nanotubes (CNT) coated bacterial cellulose (BC) bundle	399:503	In order to overcome the challenges, a novel technique has been developed to produce a long, sensitive and high tensile strength carbon nanotubes (CNT) coated bacterial cellulose (BC) bundle.
25570974	0	9	theme	suspended	98:106	arg1	resonator					108:116	suspended resonator	98:116	suspended resonator	98:116	MWCNTs-like protection layer formation on bacterial cellulose bundles as a potential material for suspended resonator.
25570974	7	10	theme	layer	1004:1008	arg1	formation					953:961	the formation	949:961	the formation of the continuous MWCNTs-like protection layer on BC surface	949:1022	This indicates the formation of the continuous MWCNTs-like protection layer on BC surface.
25570974	8	11	theme	-9	1245:1246	arg1	A					1249:1249	10(-9) A	1242:1249	10(-9) A of long BC/MWCNTs composite bundles	1242:1285	Electrical properties of the BC/MWCNTs bundles showed linearity from -6 V to +6 V. Composites with BC treated by higher ultrasonic powers, 100 W, show higher conductivity comparing to 80 W. Sensitivity from 10(-7) to 10(-9) A of long BC/MWCNTs composite bundles is reported in this paper.
25570974	8	12	theme	W.	1212:1213	arg1	Sensitivity					1215:1225	80 W. Sensitivity	1209:1225	80 W. Sensitivity from 10(-7) to 10(-9) A of long BC/MWCNTs composite bundles	1209:1285	Electrical properties of the BC/MWCNTs bundles showed linearity from -6 V to +6 V. Composites with BC treated by higher ultrasonic powers, 100 W, show higher conductivity comparing to 80 W. Sensitivity from 10(-7) to 10(-9) A of long BC/MWCNTs composite bundles is reported in this paper.
25570974	7	13	from	formation	953:961	arg1	surface					1016:1022	BC surface	1013:1022	BC surface	1013:1022	This indicates the formation of the continuous MWCNTs-like protection layer on BC surface.
25570974	3	14	theme	novel	353:357	arg1	technique					359:367	a novel technique	351:367	a novel technique	351:367	In order to overcome the challenges, a novel technique has been developed to produce a long, sensitive and high tensile strength carbon nanotubes (CNT) coated bacterial cellulose (BC) bundle.
25570974	8	15	from	Composites	1108:1117	arg1	linearity					1079:1087	linearity	1079:1087	linearity from -6 V to +6 V. Composites	1079:1117	Electrical properties of the BC/MWCNTs bundles showed linearity from -6 V to +6 V. Composites with BC treated by higher ultrasonic powers, 100 W, show higher conductivity comparing to 80 W. Sensitivity from 10(-7) to 10(-9) A of long BC/MWCNTs composite bundles is reported in this paper.
25570974	8	16	dep	powers	1156:1161	arg1	show					1171:1174	show	1171:1174	show higher conductivity comparing to 80 W. Sensitivity from 10(-7) to 10(-9) A of long BC/MWCNTs composite bundles is reported in this paper	1171:1311	Electrical properties of the BC/MWCNTs bundles showed linearity from -6 V to +6 V. Composites with BC treated by higher ultrasonic powers, 100 W, show higher conductivity comparing to 80 W. Sensitivity from 10(-7) to 10(-9) A of long BC/MWCNTs composite bundles is reported in this paper.
25570974	6	17	theme	carbonyl	905:912	arg1	peak					866:869	peak	866:869	peak of hydroxyl (-OH, 3700 m(-1)) and carbonyl (C=O, 1600 cm(-1))	866:931	Raman spectroscopy shows a drop at peak of hydroxyl (-OH, 3700 m(-1)) and carbonyl (C=O, 1600 cm(-1)).
25570974	6	18	from	peak	866:869	arg1	drop					858:861	a drop	856:861	a drop at peak of hydroxyl (-OH, 3700 m(-1)) and carbonyl (C=O, 1600 cm(-1))	856:931	Raman spectroscopy shows a drop at peak of hydroxyl (-OH, 3700 m(-1)) and carbonyl (C=O, 1600 cm(-1)).
25570974	7	19	theme	MWCNTs-like	981:991	arg1	layer					1004:1008	the continuous MWCNTs-like protection layer	966:1008	the continuous MWCNTs-like protection layer	966:1008	This indicates the formation of the continuous MWCNTs-like protection layer on BC surface.
25570974	8	20	theme	ultrasonic	1145:1154	arg1	powers					1156:1161	higher ultrasonic powers	1138:1161	higher ultrasonic powers	1138:1161	Electrical properties of the BC/MWCNTs bundles showed linearity from -6 V to +6 V. Composites with BC treated by higher ultrasonic powers, 100 W, show higher conductivity comparing to 80 W. Sensitivity from 10(-7) to 10(-9) A of long BC/MWCNTs composite bundles is reported in this paper.
25570974	8	20	theme	ultrasonic	1145:1154	arg1	W					1168:1168	100 W	1164:1168	100 W	1164:1168	Electrical properties of the BC/MWCNTs bundles showed linearity from -6 V to +6 V. Composites with BC treated by higher ultrasonic powers, 100 W, show higher conductivity comparing to 80 W. Sensitivity from 10(-7) to 10(-9) A of long BC/MWCNTs composite bundles is reported in this paper.
25570974	8	21	theme	-6	1094:1095	arg1	V					1097:1097	V	1097:1097	V	1097:1097	Electrical properties of the BC/MWCNTs bundles showed linearity from -6 V to +6 V. Composites with BC treated by higher ultrasonic powers, 100 W, show higher conductivity comparing to 80 W. Sensitivity from 10(-7) to 10(-9) A of long BC/MWCNTs composite bundles is reported in this paper.
25570974	8	22	theme	V.	1105:1106	arg1	Composites					1108:1117	-6 V to +6 V. Composites	1094:1117	-6 V to +6 V. Composites	1094:1117	Electrical properties of the BC/MWCNTs bundles showed linearity from -6 V to +6 V. Composites with BC treated by higher ultrasonic powers, 100 W, show higher conductivity comparing to 80 W. Sensitivity from 10(-7) to 10(-9) A of long BC/MWCNTs composite bundles is reported in this paper.
25570974	6	23	dep	hydroxyl	874:881	arg1	m					894:894	3700 m	889:894	3700 m(-1)	889:898	Raman spectroscopy shows a drop at peak of hydroxyl (-OH, 3700 m(-1)) and carbonyl (C=O, 1600 cm(-1)).
25570974	6	23	dep	hydroxyl	874:881	arg1	-OH					884:886	-OH	884:886	-OH	884:886	Raman spectroscopy shows a drop at peak of hydroxyl (-OH, 3700 m(-1)) and carbonyl (C=O, 1600 cm(-1)).
25570974	4	24	theme	carboxyl	568:575	arg1	MWCNTs-COOH					623:633	MWCNTs-COOH	623:633	MWCNTs-COOH	623:633	This study demonstrates the use of ultrasonication to perform carboxyl functionalized multi-walled carbon nanotubes (MWCNTs-COOH) self-assembling on the surface of BC bundles (BC/MWCNTs) via hydrogen bonds.
25570974	4	24	theme	carboxyl	568:575	arg1	nanotubes					612:620	carboxyl functionalized multi-walled carbon nanotubes	568:620	carboxyl functionalized multi-walled carbon nanotubes (MWCNTs-COOH) self-assembling on the surface of BC bundles (BC/MWCNTs)	568:691	This study demonstrates the use of ultrasonication to perform carboxyl functionalized multi-walled carbon nanotubes (MWCNTs-COOH) self-assembling on the surface of BC bundles (BC/MWCNTs) via hydrogen bonds.
25570974	1	25	theme	sensitive	167:175	arg1	detector					177:184	a sensitive detector	165:184	a sensitive detector for chemical and biological applications	165:225	Suspended carbon naotubes (CNTs) resonator is a sensitive detector for chemical and biological applications.
25570974	1	25	theme	sensitive	167:175	arg1	resonator					152:160	Suspended carbon naotubes (CNTs) resonator	119:160	Suspended carbon naotubes (CNTs) resonator	119:160	Suspended carbon naotubes (CNTs) resonator is a sensitive detector for chemical and biological applications.
25570974	0	26	theme	protection	12:21	arg1	formation					29:37	MWCNTs-like protection layer formation	0:37	MWCNTs-like protection layer formation on bacterial cellulose	0:60	MWCNTs-like protection layer formation on bacterial cellulose bundles as a potential material for suspended resonator.
25570974	4	27	theme	self-assembling	636:650	arg1	MWCNTs-COOH					623:633	MWCNTs-COOH	623:633	MWCNTs-COOH	623:633	This study demonstrates the use of ultrasonication to perform carboxyl functionalized multi-walled carbon nanotubes (MWCNTs-COOH) self-assembling on the surface of BC bundles (BC/MWCNTs) via hydrogen bonds.
25570974	4	27	theme	self-assembling	636:650	arg1	nanotubes					612:620	carboxyl functionalized multi-walled carbon nanotubes	568:620	carboxyl functionalized multi-walled carbon nanotubes (MWCNTs-COOH) self-assembling on the surface of BC bundles (BC/MWCNTs)	568:691	This study demonstrates the use of ultrasonication to perform carboxyl functionalized multi-walled carbon nanotubes (MWCNTs-COOH) self-assembling on the surface of BC bundles (BC/MWCNTs) via hydrogen bonds.
25570974	9	28	theme	nanotubes	1379:1387	arg1	resonator					1389:1397	carbon nanotubes resonator	1372:1397	carbon nanotubes resonator	1372:1397	This technique may be competitive to the current state of carbon nanotubes resonator.
25570974	0	29	theme	MWCNTs-like	0:10	arg1	formation					29:37	MWCNTs-like protection layer formation	0:37	MWCNTs-like protection layer formation on bacterial cellulose	0:60	MWCNTs-like protection layer formation on bacterial cellulose bundles as a potential material for suspended resonator.
25570974	7	30	theme	continuous	970:979	arg1	layer					1004:1008	the continuous MWCNTs-like protection layer	966:1008	the continuous MWCNTs-like protection layer	966:1008	This indicates the formation of the continuous MWCNTs-like protection layer on BC surface.
25570974	9	31	theme	resonator	1389:1397	arg1	state					1363:1367	the current state	1351:1367	the current state of carbon nanotubes resonator	1351:1397	This technique may be competitive to the current state of carbon nanotubes resonator.
25570974	3	32	theme	sensitive	407:415	arg1	bundle					498:503	a long, sensitive and high tensile strength carbon nanotubes (CNT) coated bacterial cellulose (BC) bundle	399:503	a long, sensitive and high tensile strength carbon nanotubes (CNT) coated bacterial cellulose (BC) bundle	399:503	In order to overcome the challenges, a novel technique has been developed to produce a long, sensitive and high tensile strength carbon nanotubes (CNT) coated bacterial cellulose (BC) bundle.
25570974	8	33	theme	bundles	1064:1070	arg1	properties					1036:1045	Electrical properties	1025:1045	Electrical properties of the BC/MWCNTs bundles	1025:1070	Electrical properties of the BC/MWCNTs bundles showed linearity from -6 V to +6 V. Composites with BC treated by higher ultrasonic powers, 100 W, show higher conductivity comparing to 80 W. Sensitivity from 10(-7) to 10(-9) A of long BC/MWCNTs composite bundles is reported in this paper.
25570974	8	34	theme	+6	1102:1103	arg1	V					1097:1097	V	1097:1097	V	1097:1097	Electrical properties of the BC/MWCNTs bundles showed linearity from -6 V to +6 V. Composites with BC treated by higher ultrasonic powers, 100 W, show higher conductivity comparing to 80 W. Sensitivity from 10(-7) to 10(-9) A of long BC/MWCNTs composite bundles is reported in this paper.
25570974	3	35	theme	carbon	443:448	arg1	CNT					461:463	CNT	461:463	CNT	461:463	In order to overcome the challenges, a novel technique has been developed to produce a long, sensitive and high tensile strength carbon nanotubes (CNT) coated bacterial cellulose (BC) bundle.
25570974	3	35	theme	carbon	443:448	arg1	nanotubes					450:458	high tensile strength carbon nanotubes	421:458	high tensile strength carbon nanotubes (CNT)	421:464	In order to overcome the challenges, a novel technique has been developed to produce a long, sensitive and high tensile strength carbon nanotubes (CNT) coated bacterial cellulose (BC) bundle.
25570974	0	36	theme	layer	23:27	arg1	formation					29:37	MWCNTs-like protection layer formation	0:37	MWCNTs-like protection layer formation on bacterial cellulose	0:60	MWCNTs-like protection layer formation on bacterial cellulose bundles as a potential material for suspended resonator.
25570974	8	37	theme	composite	1269:1277	arg1	bundles					1279:1285	long BC/MWCNTs composite bundles	1254:1285	long BC/MWCNTs composite bundles	1254:1285	Electrical properties of the BC/MWCNTs bundles showed linearity from -6 V to +6 V. Composites with BC treated by higher ultrasonic powers, 100 W, show higher conductivity comparing to 80 W. Sensitivity from 10(-7) to 10(-9) A of long BC/MWCNTs composite bundles is reported in this paper.
25570974	4	38	theme	bundles	673:679	arg1	surface					659:665	the surface	655:665	the surface of BC bundles (BC/MWCNTs)	655:691	This study demonstrates the use of ultrasonication to perform carboxyl functionalized multi-walled carbon nanotubes (MWCNTs-COOH) self-assembling on the surface of BC bundles (BC/MWCNTs) via hydrogen bonds.
25570974	8	39	theme	higher	1138:1143	arg1	powers					1156:1161	higher ultrasonic powers	1138:1161	higher ultrasonic powers	1138:1161	Electrical properties of the BC/MWCNTs bundles showed linearity from -6 V to +6 V. Composites with BC treated by higher ultrasonic powers, 100 W, show higher conductivity comparing to 80 W. Sensitivity from 10(-7) to 10(-9) A of long BC/MWCNTs composite bundles is reported in this paper.
25570974	8	39	theme	higher	1138:1143	arg1	W					1168:1168	100 W	1164:1168	100 W	1164:1168	Electrical properties of the BC/MWCNTs bundles showed linearity from -6 V to +6 V. Composites with BC treated by higher ultrasonic powers, 100 W, show higher conductivity comparing to 80 W. Sensitivity from 10(-7) to 10(-9) A of long BC/MWCNTs composite bundles is reported in this paper.
25570974	0	40	theme	bacterial	42:50	arg1	cellulose					52:60	bacterial cellulose	42:60	bacterial cellulose	42:60	MWCNTs-like protection layer formation on bacterial cellulose bundles as a potential material for suspended resonator.
25570974	3	41	theme	coated	466:471	arg1	bundle					498:503	a long, sensitive and high tensile strength carbon nanotubes (CNT) coated bacterial cellulose (BC) bundle	399:503	a long, sensitive and high tensile strength carbon nanotubes (CNT) coated bacterial cellulose (BC) bundle	399:503	In order to overcome the challenges, a novel technique has been developed to produce a long, sensitive and high tensile strength carbon nanotubes (CNT) coated bacterial cellulose (BC) bundle.
25570974	3	42	theme	tensile	426:432	arg1	CNT					461:463	CNT	461:463	CNT	461:463	In order to overcome the challenges, a novel technique has been developed to produce a long, sensitive and high tensile strength carbon nanotubes (CNT) coated bacterial cellulose (BC) bundle.
25570974	3	42	theme	tensile	426:432	arg1	nanotubes					450:458	high tensile strength carbon nanotubes	421:458	high tensile strength carbon nanotubes (CNT)	421:464	In order to overcome the challenges, a novel technique has been developed to produce a long, sensitive and high tensile strength carbon nanotubes (CNT) coated bacterial cellulose (BC) bundle.
25570974	1	43	theme	biological	203:212	arg1	applications					214:225	chemical and biological applications	190:225	chemical and biological applications	190:225	Suspended carbon naotubes (CNTs) resonator is a sensitive detector for chemical and biological applications.
25570974	3	44	theme	long	401:404	arg1	bundle					498:503	a long, sensitive and high tensile strength carbon nanotubes (CNT) coated bacterial cellulose (BC) bundle	399:503	a long, sensitive and high tensile strength carbon nanotubes (CNT) coated bacterial cellulose (BC) bundle	399:503	In order to overcome the challenges, a novel technique has been developed to produce a long, sensitive and high tensile strength carbon nanotubes (CNT) coated bacterial cellulose (BC) bundle.
25570974	6	45	theme	Raman	831:835	arg1	spectroscopy					837:848	Raman spectroscopy	831:848	Raman spectroscopy	831:848	Raman spectroscopy shows a drop at peak of hydroxyl (-OH, 3700 m(-1)) and carbonyl (C=O, 1600 cm(-1)).
25570974	3	46	theme	strength	434:441	arg1	CNT					461:463	CNT	461:463	CNT	461:463	In order to overcome the challenges, a novel technique has been developed to produce a long, sensitive and high tensile strength carbon nanotubes (CNT) coated bacterial cellulose (BC) bundle.
25570974	3	46	theme	strength	434:441	arg1	nanotubes					450:458	high tensile strength carbon nanotubes	421:458	high tensile strength carbon nanotubes (CNT)	421:464	In order to overcome the challenges, a novel technique has been developed to produce a long, sensitive and high tensile strength carbon nanotubes (CNT) coated bacterial cellulose (BC) bundle.
25570974	8	47	theme	BC/MWCNTs	1054:1062	arg1	bundles					1064:1070	the BC/MWCNTs bundles	1050:1070	the BC/MWCNTs bundles	1050:1070	Electrical properties of the BC/MWCNTs bundles showed linearity from -6 V to +6 V. Composites with BC treated by higher ultrasonic powers, 100 W, show higher conductivity comparing to 80 W. Sensitivity from 10(-7) to 10(-9) A of long BC/MWCNTs composite bundles is reported in this paper.
25570974	8	48	dep	V	1097:1097	arg1	to					1099:1100	to	1099:1100	to	1099:1100	Electrical properties of the BC/MWCNTs bundles showed linearity from -6 V to +6 V. Composites with BC treated by higher ultrasonic powers, 100 W, show higher conductivity comparing to 80 W. Sensitivity from 10(-7) to 10(-9) A of long BC/MWCNTs composite bundles is reported in this paper.
25570974	8	49	theme	bundles	1279:1285	arg1	A					1249:1249	10(-9) A	1242:1249	10(-9) A of long BC/MWCNTs composite bundles	1242:1285	Electrical properties of the BC/MWCNTs bundles showed linearity from -6 V to +6 V. Composites with BC treated by higher ultrasonic powers, 100 W, show higher conductivity comparing to 80 W. Sensitivity from 10(-7) to 10(-9) A of long BC/MWCNTs composite bundles is reported in this paper.
25570974	2	50	theme	Small	228:232	arg1	sizes					234:238	Small sizes	228:238	Small sizes of CNTs	228:246	Small sizes of CNTs can enhance sensitivity, but increase complexity for fabrication.
25570974	1	51	theme	Suspended	119:127	arg1	naotubes					136:143	Suspended carbon naotubes	119:143	Suspended carbon naotubes (CNTs) resonator	119:160	Suspended carbon naotubes (CNTs) resonator is a sensitive detector for chemical and biological applications.
25570974	1	51	theme	Suspended	119:127	arg1	CNTs					146:149	CNTs	146:149	CNTs	146:149	Suspended carbon naotubes (CNTs) resonator is a sensitive detector for chemical and biological applications.
25570974	5	52	theme	long	781:784	arg1	bundles					796:802	the long BC/MWCNTs bundles	777:802	the long BC/MWCNTs bundles ranging from 30 to 100 μm	777:828	Ultrasonication can disrupt dense cellulose network and produce the long BC/MWCNTs bundles ranging from 30 to 100 μm.
25570974	6	53	theme	3700	889:892	arg1	m					894:894	3700 m	889:894	3700 m(-1)	889:898	Raman spectroscopy shows a drop at peak of hydroxyl (-OH, 3700 m(-1)) and carbonyl (C=O, 1600 cm(-1)).
25570974	6	53	theme	3700	889:892	arg1	-1					896:897	-1	896:897	-1	896:897	Raman spectroscopy shows a drop at peak of hydroxyl (-OH, 3700 m(-1)) and carbonyl (C=O, 1600 cm(-1)).
25570974	6	53	theme	3700	889:892	arg1	-OH					884:886	-OH	884:886	-OH	884:886	Raman spectroscopy shows a drop at peak of hydroxyl (-OH, 3700 m(-1)) and carbonyl (C=O, 1600 cm(-1)).
25570974	7	54	theme	protection	993:1002	arg1	layer					1004:1008	the continuous MWCNTs-like protection layer	966:1008	the continuous MWCNTs-like protection layer	966:1008	This indicates the formation of the continuous MWCNTs-like protection layer on BC surface.
25570974	1	55	theme	carbon	129:134	arg1	naotubes					136:143	Suspended carbon naotubes	119:143	Suspended carbon naotubes (CNTs) resonator	119:160	Suspended carbon naotubes (CNTs) resonator is a sensitive detector for chemical and biological applications.
25570974	1	55	theme	carbon	129:134	arg1	CNTs					146:149	CNTs	146:149	CNTs	146:149	Suspended carbon naotubes (CNTs) resonator is a sensitive detector for chemical and biological applications.
25570974	8	56	theme	BC/MWCNTs	1259:1267	arg1	bundles					1279:1285	long BC/MWCNTs composite bundles	1254:1285	long BC/MWCNTs composite bundles	1254:1285	Electrical properties of the BC/MWCNTs bundles showed linearity from -6 V to +6 V. Composites with BC treated by higher ultrasonic powers, 100 W, show higher conductivity comparing to 80 W. Sensitivity from 10(-7) to 10(-9) A of long BC/MWCNTs composite bundles is reported in this paper.
25570974	5	57	dep	100	823:825	arg1	to					820:821	to	820:821	to	820:821	Ultrasonication can disrupt dense cellulose network and produce the long BC/MWCNTs bundles ranging from 30 to 100 μm.
25570974	1	58	theme	naotubes	136:143	arg1	detector					177:184	a sensitive detector	165:184	a sensitive detector for chemical and biological applications	165:225	Suspended carbon naotubes (CNTs) resonator is a sensitive detector for chemical and biological applications.
25570974	1	58	theme	naotubes	136:143	arg1	resonator					152:160	Suspended carbon naotubes (CNTs) resonator	119:160	Suspended carbon naotubes (CNTs) resonator	119:160	Suspended carbon naotubes (CNTs) resonator is a sensitive detector for chemical and biological applications.
25570974	4	59	theme	functionalized	577:590	arg1	MWCNTs-COOH					623:633	MWCNTs-COOH	623:633	MWCNTs-COOH	623:633	This study demonstrates the use of ultrasonication to perform carboxyl functionalized multi-walled carbon nanotubes (MWCNTs-COOH) self-assembling on the surface of BC bundles (BC/MWCNTs) via hydrogen bonds.
25570974	4	59	theme	functionalized	577:590	arg1	nanotubes					612:620	carboxyl functionalized multi-walled carbon nanotubes	568:620	carboxyl functionalized multi-walled carbon nanotubes (MWCNTs-COOH) self-assembling on the surface of BC bundles (BC/MWCNTs)	568:691	This study demonstrates the use of ultrasonication to perform carboxyl functionalized multi-walled carbon nanotubes (MWCNTs-COOH) self-assembling on the surface of BC bundles (BC/MWCNTs) via hydrogen bonds.
25570974	9	60	theme	carbon	1372:1377	arg1	resonator					1389:1397	carbon nanotubes resonator	1372:1397	carbon nanotubes resonator	1372:1397	This technique may be competitive to the current state of carbon nanotubes resonator.
25570974	9	61	theme	current	1355:1361	arg1	state					1363:1367	the current state	1351:1367	the current state of carbon nanotubes resonator	1351:1397	This technique may be competitive to the current state of carbon nanotubes resonator.
25570974	8	62	theme	higher	1176:1181	arg1	conductivity					1183:1194	higher conductivity	1176:1194	higher conductivity comparing to 80 W. Sensitivity from 10(-7) to 10(-9) A of long BC/MWCNTs composite bundles	1176:1285	Electrical properties of the BC/MWCNTs bundles showed linearity from -6 V to +6 V. Composites with BC treated by higher ultrasonic powers, 100 W, show higher conductivity comparing to 80 W. Sensitivity from 10(-7) to 10(-9) A of long BC/MWCNTs composite bundles is reported in this paper.
25570974	4	63	theme	hydrogen	697:704	arg1	bonds					706:710	hydrogen bonds	697:710	hydrogen bonds	697:710	This study demonstrates the use of ultrasonication to perform carboxyl functionalized multi-walled carbon nanotubes (MWCNTs-COOH) self-assembling on the surface of BC bundles (BC/MWCNTs) via hydrogen bonds.
25570974	8	64	theme	Electrical	1025:1034	arg1	properties					1036:1045	Electrical properties	1025:1045	Electrical properties of the BC/MWCNTs bundles	1025:1070	Electrical properties of the BC/MWCNTs bundles showed linearity from -6 V to +6 V. Composites with BC treated by higher ultrasonic powers, 100 W, show higher conductivity comparing to 80 W. Sensitivity from 10(-7) to 10(-9) A of long BC/MWCNTs composite bundles is reported in this paper.
25570974	8	65	theme	long	1254:1257	arg1	bundles					1279:1285	long BC/MWCNTs composite bundles	1254:1285	long BC/MWCNTs composite bundles	1254:1285	Electrical properties of the BC/MWCNTs bundles showed linearity from -6 V to +6 V. Composites with BC treated by higher ultrasonic powers, 100 W, show higher conductivity comparing to 80 W. Sensitivity from 10(-7) to 10(-9) A of long BC/MWCNTs composite bundles is reported in this paper.
25570974	1	66	theme	chemical	190:197	arg1	applications					214:225	chemical and biological applications	190:225	chemical and biological applications	190:225	Suspended carbon naotubes (CNTs) resonator is a sensitive detector for chemical and biological applications.
25570974	4	67	theme	BC	670:671	arg1	bundles					673:679	BC bundles	670:679	BC bundles (BC/MWCNTs)	670:691	This study demonstrates the use of ultrasonication to perform carboxyl functionalized multi-walled carbon nanotubes (MWCNTs-COOH) self-assembling on the surface of BC bundles (BC/MWCNTs) via hydrogen bonds.
25570974	4	67	theme	BC	670:671	arg1	BC/MWCNTs					682:690	BC/MWCNTs	682:690	BC/MWCNTs	682:690	This study demonstrates the use of ultrasonication to perform carboxyl functionalized multi-walled carbon nanotubes (MWCNTs-COOH) self-assembling on the surface of BC bundles (BC/MWCNTs) via hydrogen bonds.
25570974	3	68	theme	high	421:424	arg1	CNT					461:463	CNT	461:463	CNT	461:463	In order to overcome the challenges, a novel technique has been developed to produce a long, sensitive and high tensile strength carbon nanotubes (CNT) coated bacterial cellulose (BC) bundle.
25570974	3	68	theme	high	421:424	arg1	nanotubes					450:458	high tensile strength carbon nanotubes	421:458	high tensile strength carbon nanotubes (CNT)	421:464	In order to overcome the challenges, a novel technique has been developed to produce a long, sensitive and high tensile strength carbon nanotubes (CNT) coated bacterial cellulose (BC) bundle.
25570974	4	69	theme	carbon	605:610	arg1	MWCNTs-COOH					623:633	MWCNTs-COOH	623:633	MWCNTs-COOH	623:633	This study demonstrates the use of ultrasonication to perform carboxyl functionalized multi-walled carbon nanotubes (MWCNTs-COOH) self-assembling on the surface of BC bundles (BC/MWCNTs) via hydrogen bonds.
25570974	4	69	theme	carbon	605:610	arg1	nanotubes					612:620	carboxyl functionalized multi-walled carbon nanotubes	568:620	carboxyl functionalized multi-walled carbon nanotubes (MWCNTs-COOH) self-assembling on the surface of BC bundles (BC/MWCNTs)	568:691	This study demonstrates the use of ultrasonication to perform carboxyl functionalized multi-walled carbon nanotubes (MWCNTs-COOH) self-assembling on the surface of BC bundles (BC/MWCNTs) via hydrogen bonds.
25570974	5	70	theme	dense	741:745	arg1	network					757:763	dense cellulose network	741:763	dense cellulose network	741:763	Ultrasonication can disrupt dense cellulose network and produce the long BC/MWCNTs bundles ranging from 30 to 100 μm.
25570974	0	71	from	formation	29:37	arg1	cellulose					52:60	bacterial cellulose	42:60	bacterial cellulose	42:60	MWCNTs-like protection layer formation on bacterial cellulose bundles as a potential material for suspended resonator.
25570974	2	72	theme	CNTs	243:246	arg1	sizes					234:238	Small sizes	228:238	Small sizes of CNTs	228:246	Small sizes of CNTs can enhance sensitivity, but increase complexity for fabrication.
25570974	5	73	theme	BC/MWCNTs	786:794	arg1	bundles					796:802	the long BC/MWCNTs bundles	777:802	the long BC/MWCNTs bundles ranging from 30 to 100 μm	777:828	Ultrasonication can disrupt dense cellulose network and produce the long BC/MWCNTs bundles ranging from 30 to 100 μm.
25570974	4	74	theme	multi-walled	592:603	arg1	MWCNTs-COOH					623:633	MWCNTs-COOH	623:633	MWCNTs-COOH	623:633	This study demonstrates the use of ultrasonication to perform carboxyl functionalized multi-walled carbon nanotubes (MWCNTs-COOH) self-assembling on the surface of BC bundles (BC/MWCNTs) via hydrogen bonds.
25570974	4	74	theme	multi-walled	592:603	arg1	nanotubes					612:620	carboxyl functionalized multi-walled carbon nanotubes	568:620	carboxyl functionalized multi-walled carbon nanotubes (MWCNTs-COOH) self-assembling on the surface of BC bundles (BC/MWCNTs)	568:691	This study demonstrates the use of ultrasonication to perform carboxyl functionalized multi-walled carbon nanotubes (MWCNTs-COOH) self-assembling on the surface of BC bundles (BC/MWCNTs) via hydrogen bonds.
26256361	6	0	theme	variable	978:985	arg1	stability					991:999	variable NMP stability	978:999	variable NMP stability on the order of two weeks or more at different ionic strength	978:1061	The stability of the NMPs is related to the particle composition and the aqueous solution conditions, as evidenced by variable NMP stability on the order of two weeks or more at different ionic strength.
26256361	4	1	theme	±	665:665	arg1	0.28					673:676	0.28	673:676	0.28	673:676	The average size and polydispersity index (in parentheses) vary and are reported for the chitosan-based particles from 243.0 ± 1 nm (0.28) to 424 ± 14 nm (0.33), according to DLS measurements at ambient conditions.
26256361	4	1	theme	±	665:665	arg1	nm					669:670	243.0 ± 1 nm	659:670	243.0 ± 1 nm (0.28) to 424 ± 14 nm (0.33)	659:699	The average size and polydispersity index (in parentheses) vary and are reported for the chitosan-based particles from 243.0 ± 1 nm (0.28) to 424 ± 14 nm (0.33), according to DLS measurements at ambient conditions.
26256361	4	2	from	index	576:580	arg1	parentheses					586:596	parentheses	586:596	parentheses	586:596	The average size and polydispersity index (in parentheses) vary and are reported for the chitosan-based particles from 243.0 ± 1 nm (0.28) to 424 ± 14 nm (0.33), according to DLS measurements at ambient conditions.
26256361	3	3	theme	urea	515:518	arg1	solution					520:527	the aqueous urea solution	503:527	the aqueous urea solution	503:527	According to DLS, the particle size was observed to increase as the concentration of the aqueous urea solution increased.
26256361	4	4	theme	±	686:686	arg1	0.33					695:698	0.33	695:698	0.33	695:698	The average size and polydispersity index (in parentheses) vary and are reported for the chitosan-based particles from 243.0 ± 1 nm (0.28) to 424 ± 14 nm (0.33), according to DLS measurements at ambient conditions.
26256361	4	4	theme	±	686:686	arg1	nm					691:692	424 ± 14 nm	682:692	424 ± 14 nm (0.33)	682:699	The average size and polydispersity index (in parentheses) vary and are reported for the chitosan-based particles from 243.0 ± 1 nm (0.28) to 424 ± 14 nm (0.33), according to DLS measurements at ambient conditions.
26256361	3	5	theme	solution	520:527	arg1	concentration					486:498	the concentration	482:498	the concentration of the aqueous urea solution	482:527	According to DLS, the particle size was observed to increase as the concentration of the aqueous urea solution increased.
26256361	6	6	theme	more	1030:1033	arg1	order					1008:1012	the order	1004:1012	the order of two weeks or more at different ionic strength	1004:1061	The stability of the NMPs is related to the particle composition and the aqueous solution conditions, as evidenced by variable NMP stability on the order of two weeks or more at different ionic strength.
26256361	1	7	with	chitosan	180:187	arg1	tripolyphosphate					269:284	tripolyphosphate	269:284	tripolyphosphate	269:284	Composite nano-microparticles were prepared in aqueous solution that contain chitosan or modified (carboxymethyl) chitosan with polyanion species such as alginate or tripolyphosphate, respectively.
26256361	1	7	with	chitosan	180:187	arg1	species					241:247	polyanion species	231:247	polyanion species such as alginate or tripolyphosphate	231:284	Composite nano-microparticles were prepared in aqueous solution that contain chitosan or modified (carboxymethyl) chitosan with polyanion species such as alginate or tripolyphosphate, respectively.
26256361	1	7	with	chitosan	180:187	arg1	alginate					257:264	alginate	257:264	alginate	257:264	Composite nano-microparticles were prepared in aqueous solution that contain chitosan or modified (carboxymethyl) chitosan with polyanion species such as alginate or tripolyphosphate, respectively.
26256361	2	8	theme	particles	318:326	arg1	types					309:313	Several types	301:313	Several types of particles	301:326	Several types of particles were prepared and characterized by (1)H/(31)P NMR spectroscopy, IR spectroscopy, and DLS.
26256361	6	9	theme	aqueous	933:939	arg1	conditions					950:959	the aqueous solution conditions	929:959	the aqueous solution conditions	929:959	The stability of the NMPs is related to the particle composition and the aqueous solution conditions, as evidenced by variable NMP stability on the order of two weeks or more at different ionic strength.
26256361	6	10	theme	different	1038:1046	arg1	strength					1054:1061	different ionic strength	1038:1061	different ionic strength	1038:1061	The stability of the NMPs is related to the particle composition and the aqueous solution conditions, as evidenced by variable NMP stability on the order of two weeks or more at different ionic strength.
26256361	2	11	theme	IR	392:393	arg1	spectroscopy					395:406	IR spectroscopy	392:406	IR spectroscopy	392:406	Several types of particles were prepared and characterized by (1)H/(31)P NMR spectroscopy, IR spectroscopy, and DLS.
26256361	3	12	theme	aqueous	507:513	arg1	solution					520:527	the aqueous urea solution	503:527	the aqueous urea solution	503:527	According to DLS, the particle size was observed to increase as the concentration of the aqueous urea solution increased.
26256361	6	13	from	strength	1054:1061	arg1	order					1008:1012	the order	1004:1012	the order of two weeks or more at different ionic strength	1004:1061	The stability of the NMPs is related to the particle composition and the aqueous solution conditions, as evidenced by variable NMP stability on the order of two weeks or more at different ionic strength.
26256361	6	13	from	strength	1054:1061	arg1	weeks					1021:1025	two weeks	1017:1025	two weeks	1017:1025	The stability of the NMPs is related to the particle composition and the aqueous solution conditions, as evidenced by variable NMP stability on the order of two weeks or more at different ionic strength.
26256361	1	14	contain	contain	172:178	arg1	solution					158:165	aqueous solution	150:165	aqueous solution that contain chitosan or modified (carboxymethyl) chitosan with polyanion species such as alginate or tripolyphosphate, respectively	150:298	Composite nano-microparticles were prepared in aqueous solution that contain chitosan or modified (carboxymethyl) chitosan with polyanion species such as alginate or tripolyphosphate, respectively.
26256361	1	14	contain	contain	172:178	arg2	modified					192:199	modified	192:199	modified	192:199	Composite nano-microparticles were prepared in aqueous solution that contain chitosan or modified (carboxymethyl) chitosan with polyanion species such as alginate or tripolyphosphate, respectively.
26256361	1	14	contain	contain	172:178	arg2	chitosan					180:187	chitosan	180:187	chitosan	180:187	Composite nano-microparticles were prepared in aqueous solution that contain chitosan or modified (carboxymethyl) chitosan with polyanion species such as alginate or tripolyphosphate, respectively.
26256361	1	14	contain	contain	172:178	arg2	carboxymethyl					202:214	carboxymethyl	202:214	carboxymethyl	202:214	Composite nano-microparticles were prepared in aqueous solution that contain chitosan or modified (carboxymethyl) chitosan with polyanion species such as alginate or tripolyphosphate, respectively.
26256361	1	15	theme	Composite	103:111	arg1	nano-microparticles					113:131	Composite nano-microparticles	103:131	Composite nano-microparticles	103:131	Composite nano-microparticles were prepared in aqueous solution that contain chitosan or modified (carboxymethyl) chitosan with polyanion species such as alginate or tripolyphosphate, respectively.
26256361	5	16	theme	relative	839:846	arg1	range					853:857	the relative size range	835:857	the relative size range	835:857	Thus, the particles are herein referred to as nano-microparticles (NMPs) due to the relative size range.
26256361	2	17	theme	NMR	374:376	arg1	spectroscopy					378:389	(1)H/(31)P NMR spectroscopy	363:389	(1)H/(31)P NMR spectroscopy	363:389	Several types of particles were prepared and characterized by (1)H/(31)P NMR spectroscopy, IR spectroscopy, and DLS.
26256361	5	18	theme	size	848:851	arg1	range					853:857	the relative size range	835:857	the relative size range	835:857	Thus, the particles are herein referred to as nano-microparticles (NMPs) due to the relative size range.
26256361	3	19	theme	particle	440:447	arg1	size					449:452	the particle size	436:452	the particle size	436:452	According to DLS, the particle size was observed to increase as the concentration of the aqueous urea solution increased.
26256361	4	20	theme	average	544:550	arg1	size					552:555	The average size	540:555	The average size	540:555	The average size and polydispersity index (in parentheses) vary and are reported for the chitosan-based particles from 243.0 ± 1 nm (0.28) to 424 ± 14 nm (0.33), according to DLS measurements at ambient conditions.
26256361	1	21	dep	chitosan	180:187	arg1	chitosan					217:224	chitosan	217:224	chitosan	217:224	Composite nano-microparticles were prepared in aqueous solution that contain chitosan or modified (carboxymethyl) chitosan with polyanion species such as alginate or tripolyphosphate, respectively.
26256361	1	22	theme	polyanion	231:239	arg1	tripolyphosphate					269:284	tripolyphosphate	269:284	tripolyphosphate	269:284	Composite nano-microparticles were prepared in aqueous solution that contain chitosan or modified (carboxymethyl) chitosan with polyanion species such as alginate or tripolyphosphate, respectively.
26256361	1	22	theme	polyanion	231:239	arg1	species					241:247	polyanion species	231:247	polyanion species such as alginate or tripolyphosphate	231:284	Composite nano-microparticles were prepared in aqueous solution that contain chitosan or modified (carboxymethyl) chitosan with polyanion species such as alginate or tripolyphosphate, respectively.
26256361	1	22	theme	polyanion	231:239	arg1	alginate					257:264	alginate	257:264	alginate	257:264	Composite nano-microparticles were prepared in aqueous solution that contain chitosan or modified (carboxymethyl) chitosan with polyanion species such as alginate or tripolyphosphate, respectively.
26256361	0	23	theme	composite	46:54	arg1	particles					56:64	chitosan-based composite particles	31:64	chitosan-based composite particles	31:64	Design and characterization of chitosan-based composite particles with tunable interfacial properties.
26256361	4	24	theme	chitosan-based	629:642	arg1	particles					644:652	the chitosan-based particles	625:652	the chitosan-based particles from 243.0 ± 1 nm (0.28) to 424 ± 14 nm (0.33)	625:699	The average size and polydispersity index (in parentheses) vary and are reported for the chitosan-based particles from 243.0 ± 1 nm (0.28) to 424 ± 14 nm (0.33), according to DLS measurements at ambient conditions.
26256361	0	25	theme	chitosan-based	31:44	arg1	particles					56:64	chitosan-based composite particles	31:64	chitosan-based composite particles	31:64	Design and characterization of chitosan-based composite particles with tunable interfacial properties.
26256361	4	26	from	conditions	743:752	arg1	measurements					719:730	DLS measurements	715:730	DLS measurements at ambient conditions	715:752	The average size and polydispersity index (in parentheses) vary and are reported for the chitosan-based particles from 243.0 ± 1 nm (0.28) to 424 ± 14 nm (0.33), according to DLS measurements at ambient conditions.
26256361	4	27	dep	size	552:555	arg1	index					576:580	index	576:580	index (in parentheses)	576:597	The average size and polydispersity index (in parentheses) vary and are reported for the chitosan-based particles from 243.0 ± 1 nm (0.28) to 424 ± 14 nm (0.33), according to DLS measurements at ambient conditions.
26256361	0	28	with	characterization	11:26	arg1	properties					91:100	tunable interfacial properties	71:100	tunable interfacial properties	71:100	Design and characterization of chitosan-based composite particles with tunable interfacial properties.
26256361	2	29	theme	H/	366:367	arg1	spectroscopy					378:389	(1)H/(31)P NMR spectroscopy	363:389	(1)H/(31)P NMR spectroscopy	363:389	Several types of particles were prepared and characterized by (1)H/(31)P NMR spectroscopy, IR spectroscopy, and DLS.
26256361	0	30	theme	particles	56:64	arg1	characterization					11:26	characterization	11:26	characterization	11:26	Design and characterization of chitosan-based composite particles with tunable interfacial properties.
26256361	0	30	theme	particles	56:64	arg1	Design					0:5	Design	0:5	Design	0:5	Design and characterization of chitosan-based composite particles with tunable interfacial properties.
26256361	6	31	theme	ionic	1048:1052	arg1	strength					1054:1061	different ionic strength	1038:1061	different ionic strength	1038:1061	The stability of the NMPs is related to the particle composition and the aqueous solution conditions, as evidenced by variable NMP stability on the order of two weeks or more at different ionic strength.
26256361	4	32	theme	ambient	735:741	arg1	conditions					743:752	ambient conditions	735:752	ambient conditions	735:752	The average size and polydispersity index (in parentheses) vary and are reported for the chitosan-based particles from 243.0 ± 1 nm (0.28) to 424 ± 14 nm (0.33), according to DLS measurements at ambient conditions.
26256361	1	33	theme	aqueous	150:156	arg1	solution					158:165	aqueous solution	150:165	aqueous solution that contain chitosan or modified (carboxymethyl) chitosan with polyanion species such as alginate or tripolyphosphate, respectively	150:298	Composite nano-microparticles were prepared in aqueous solution that contain chitosan or modified (carboxymethyl) chitosan with polyanion species such as alginate or tripolyphosphate, respectively.
26256361	2	34	theme	Several	301:307	arg1	types					309:313	Several types	301:313	Several types of particles	301:326	Several types of particles were prepared and characterized by (1)H/(31)P NMR spectroscopy, IR spectroscopy, and DLS.
26256361	0	35	with	Design	0:5	arg1	properties					91:100	tunable interfacial properties	71:100	tunable interfacial properties	71:100	Design and characterization of chitosan-based composite particles with tunable interfacial properties.
26256361	6	36	theme	weeks	1021:1025	arg1	order					1008:1012	the order	1004:1012	the order of two weeks or more at different ionic strength	1004:1061	The stability of the NMPs is related to the particle composition and the aqueous solution conditions, as evidenced by variable NMP stability on the order of two weeks or more at different ionic strength.
26256361	6	37	theme	NMPs	881:884	arg1	related					889:895	related	889:895	related	889:895	The stability of the NMPs is related to the particle composition and the aqueous solution conditions, as evidenced by variable NMP stability on the order of two weeks or more at different ionic strength.
26256361	6	37	theme	NMPs	881:884	arg1	stability					864:872	The stability	860:872	The stability of the NMPs	860:884	The stability of the NMPs is related to the particle composition and the aqueous solution conditions, as evidenced by variable NMP stability on the order of two weeks or more at different ionic strength.
26256361	6	38	from	order	1008:1012	arg1	strength					1054:1061	different ionic strength	1038:1061	different ionic strength	1038:1061	The stability of the NMPs is related to the particle composition and the aqueous solution conditions, as evidenced by variable NMP stability on the order of two weeks or more at different ionic strength.
26256361	6	39	theme	particle	904:911	arg1	composition					913:923	the particle composition	900:923	the particle composition	900:923	The stability of the NMPs is related to the particle composition and the aqueous solution conditions, as evidenced by variable NMP stability on the order of two weeks or more at different ionic strength.
26256361	0	40	theme	interfacial	79:89	arg1	properties					91:100	tunable interfacial properties	71:100	tunable interfacial properties	71:100	Design and characterization of chitosan-based composite particles with tunable interfacial properties.
26256361	4	41	theme	DLS	715:717	arg1	measurements					719:730	DLS measurements	715:730	DLS measurements at ambient conditions	715:752	The average size and polydispersity index (in parentheses) vary and are reported for the chitosan-based particles from 243.0 ± 1 nm (0.28) to 424 ± 14 nm (0.33), according to DLS measurements at ambient conditions.
26256361	6	42	theme	solution	941:948	arg1	conditions					950:959	the aqueous solution conditions	929:959	the aqueous solution conditions	929:959	The stability of the NMPs is related to the particle composition and the aqueous solution conditions, as evidenced by variable NMP stability on the order of two weeks or more at different ionic strength.
26256361	2	43	theme	P	372:372	arg1	spectroscopy					378:389	(1)H/(31)P NMR spectroscopy	363:389	(1)H/(31)P NMR spectroscopy	363:389	Several types of particles were prepared and characterized by (1)H/(31)P NMR spectroscopy, IR spectroscopy, and DLS.
26256361	4	44	from	nm	669:670	arg1	particles					644:652	the chitosan-based particles	625:652	the chitosan-based particles from 243.0 ± 1 nm (0.28) to 424 ± 14 nm (0.33)	625:699	The average size and polydispersity index (in parentheses) vary and are reported for the chitosan-based particles from 243.0 ± 1 nm (0.28) to 424 ± 14 nm (0.33), according to DLS measurements at ambient conditions.
26256361	0	45	theme	tunable	71:77	arg1	properties					91:100	tunable interfacial properties	71:100	tunable interfacial properties	71:100	Design and characterization of chitosan-based composite particles with tunable interfacial properties.
26256361	6	46	from	stability	991:999	arg1	order					1008:1012	the order	1004:1012	the order of two weeks or more at different ionic strength	1004:1061	The stability of the NMPs is related to the particle composition and the aqueous solution conditions, as evidenced by variable NMP stability on the order of two weeks or more at different ionic strength.
26256361	6	47	theme	NMP	987:989	arg1	stability					991:999	variable NMP stability	978:999	variable NMP stability on the order of two weeks or more at different ionic strength	978:1061	The stability of the NMPs is related to the particle composition and the aqueous solution conditions, as evidenced by variable NMP stability on the order of two weeks or more at different ionic strength.
24852819	2	0	theme	physico-chemical	456:471	arg1	properties					473:482	physico-chemical properties	456:482	physico-chemical properties	456:482	The aim of the work was to investigate the complex genetic relationships between the vegetative period, colour, size and production of seed, the composition (polysaccharides and proteins) and physico-chemical properties of soluble mucilages collected at 28 °C from seeds of 18 lines grown in St Petersburg area.
24852819	7	1	from	focus	1433:1437	arg1	yield					1451:1455	mucilage yield	1442:1455	mucilage yield	1442:1455	Relatively independent characters (form/shape, protein and galactosidase) were identified and could be combined by breeding, with a focus on mucilage yield, composition and properties.
24852819	7	1	from	focus	1433:1437	arg1	properties					1474:1483	properties	1474:1483	properties	1474:1483	Relatively independent characters (form/shape, protein and galactosidase) were identified and could be combined by breeding, with a focus on mucilage yield, composition and properties.
24852819	7	1	from	focus	1433:1437	arg1	composition					1458:1468	composition	1458:1468	composition	1458:1468	Relatively independent characters (form/shape, protein and galactosidase) were identified and could be combined by breeding, with a focus on mucilage yield, composition and properties.
24852819	5	2	contain	had	1200:1202	arg1	lines					1194:1198	Seven fibre lines	1182:1198	Seven fibre lines	1182:1198	Seven fibre lines had mucilages particularly rich in galactose.
24852819	5	2	contain	had	1200:1202	arg2	mucilages					1204:1212	mucilages	1204:1212	mucilages particularly rich in galactose	1204:1243	Seven fibre lines had mucilages particularly rich in galactose.
24852819	3	3	theme	some	823:826	arg1	production					640:649	production	640:649	production	640:649	The vegetative period duration was found to impact the size and production of seeds, the yield of mucilages, including the polysaccharides, and the galactosidase enzymes, as well as their composition (mainly the rhamnogalacturonan I moieties) and some of their properties (mainly viscosity).
24852819	3	3	theme	some	823:826	arg1	size					631:634	size	631:634	size	631:634	The vegetative period duration was found to impact the size and production of seeds, the yield of mucilages, including the polysaccharides, and the galactosidase enzymes, as well as their composition (mainly the rhamnogalacturonan I moieties) and some of their properties (mainly viscosity).
24852819	4	4	theme	fibre	912:916	arg1	lines					918:922	6 fibre lines	910:922	6 fibre lines with mucilages enriched in rhamnogalacturonan I	910:970	Data allowed to significantly distinguish 6 fibre lines with mucilages enriched in rhamnogalacturonan I, 6 lines with mucilages enriched in arabinoxylan including 5 linseeds and 1 mutated fibre-line, and 5 lines with mucilages enriched in homogalacturonan-like polymer including 4 fibre lines and 1 brown linseed.
24852819	2	5	theme	Petersburg	559:568	arg1	area					570:573	St Petersburg area	556:573	St Petersburg area	556:573	The aim of the work was to investigate the complex genetic relationships between the vegetative period, colour, size and production of seed, the composition (polysaccharides and proteins) and physico-chemical properties of soluble mucilages collected at 28 °C from seeds of 18 lines grown in St Petersburg area.
24852819	4	6	theme	brown	1167:1171	arg1	linseed					1173:1179	1 brown linseed	1165:1179	1 brown linseed	1165:1179	Data allowed to significantly distinguish 6 fibre lines with mucilages enriched in rhamnogalacturonan I, 6 lines with mucilages enriched in arabinoxylan including 5 linseeds and 1 mutated fibre-line, and 5 lines with mucilages enriched in homogalacturonan-like polymer including 4 fibre lines and 1 brown linseed.
24852819	1	7	theme	flax	141:144	arg1	seeds					146:150	flax seeds	141:150	flax seeds	141:150	Upon hydration, flax seeds secrete mucilages whose content and physico-chemical properties vary according to the genotype and environment.
24852819	0	8	theme	flax	79:82	arg1	collection					92:101	the flax genetic collection	75:101	the flax genetic collection of Vavilov Institute	75:122	Variability of seed traits and properties of soluble mucilages in lines of the flax genetic collection of Vavilov Institute.
24852819	0	9	from	Variability	0:10	arg1	lines					66:70	lines	66:70	lines of the flax genetic collection of Vavilov Institute	66:122	Variability of seed traits and properties of soluble mucilages in lines of the flax genetic collection of Vavilov Institute.
24852819	2	10	theme	St	556:557	arg1	area					570:573	St Petersburg area	556:573	St Petersburg area	556:573	The aim of the work was to investigate the complex genetic relationships between the vegetative period, colour, size and production of seed, the composition (polysaccharides and proteins) and physico-chemical properties of soluble mucilages collected at 28 °C from seeds of 18 lines grown in St Petersburg area.
24852819	3	11	dep	composition	764:774	arg1	moieties					809:816	the rhamnogalacturonan I moieties	784:816	mainly the rhamnogalacturonan I moieties	777:816	The vegetative period duration was found to impact the size and production of seeds, the yield of mucilages, including the polysaccharides, and the galactosidase enzymes, as well as their composition (mainly the rhamnogalacturonan I moieties) and some of their properties (mainly viscosity).
24852819	3	12	dep	size	631:634	arg1	the					627:629	the	627:629	the	627:629	The vegetative period duration was found to impact the size and production of seeds, the yield of mucilages, including the polysaccharides, and the galactosidase enzymes, as well as their composition (mainly the rhamnogalacturonan I moieties) and some of their properties (mainly viscosity).
24852819	4	13	with	lines	918:922	arg1	mucilages					986:994	mucilages	986:994	mucilages enriched in arabinoxylan including 5 linseeds and 1 mutated fibre-line	986:1065	Data allowed to significantly distinguish 6 fibre lines with mucilages enriched in rhamnogalacturonan I, 6 lines with mucilages enriched in arabinoxylan including 5 linseeds and 1 mutated fibre-line, and 5 lines with mucilages enriched in homogalacturonan-like polymer including 4 fibre lines and 1 brown linseed.
24852819	4	13	with	lines	918:922	arg1	mucilages					1085:1093	mucilages	1085:1093	mucilages enriched in homogalacturonan-like polymer including 4 fibre lines and 1 brown linseed	1085:1179	Data allowed to significantly distinguish 6 fibre lines with mucilages enriched in rhamnogalacturonan I, 6 lines with mucilages enriched in arabinoxylan including 5 linseeds and 1 mutated fibre-line, and 5 lines with mucilages enriched in homogalacturonan-like polymer including 4 fibre lines and 1 brown linseed.
24852819	4	13	with	lines	918:922	arg1	mucilages					929:937	mucilages	929:937	mucilages enriched in rhamnogalacturonan I	929:970	Data allowed to significantly distinguish 6 fibre lines with mucilages enriched in rhamnogalacturonan I, 6 lines with mucilages enriched in arabinoxylan including 5 linseeds and 1 mutated fibre-line, and 5 lines with mucilages enriched in homogalacturonan-like polymer including 4 fibre lines and 1 brown linseed.
24852819	8	14	theme	fibre	1634:1638	arg1	lines					1640:1644	Russian fibre lines	1626:1644	Russian fibre lines with brown seeds	1626:1661	Main-component analyses of line characters showed a large diversity in linseeds mainly due to their different origin but small variation in Russian fibre lines with brown seeds.
24852819	2	15	theme	mucilages	495:503	arg1	size					376:379	size	376:379	size	376:379	The aim of the work was to investigate the complex genetic relationships between the vegetative period, colour, size and production of seed, the composition (polysaccharides and proteins) and physico-chemical properties of soluble mucilages collected at 28 °C from seeds of 18 lines grown in St Petersburg area.
24852819	2	15	theme	mucilages	495:503	arg1	composition					409:419	the composition	405:419	the composition (polysaccharides and proteins)	405:450	The aim of the work was to investigate the complex genetic relationships between the vegetative period, colour, size and production of seed, the composition (polysaccharides and proteins) and physico-chemical properties of soluble mucilages collected at 28 °C from seeds of 18 lines grown in St Petersburg area.
24852819	2	15	theme	mucilages	495:503	arg1	period					360:365	the vegetative period	345:365	the vegetative period	345:365	The aim of the work was to investigate the complex genetic relationships between the vegetative period, colour, size and production of seed, the composition (polysaccharides and proteins) and physico-chemical properties of soluble mucilages collected at 28 °C from seeds of 18 lines grown in St Petersburg area.
24852819	2	15	theme	mucilages	495:503	arg1	properties					473:482	physico-chemical properties	456:482	physico-chemical properties	456:482	The aim of the work was to investigate the complex genetic relationships between the vegetative period, colour, size and production of seed, the composition (polysaccharides and proteins) and physico-chemical properties of soluble mucilages collected at 28 °C from seeds of 18 lines grown in St Petersburg area.
24852819	2	15	theme	mucilages	495:503	arg1	production					385:394	production	385:394	production	385:394	The aim of the work was to investigate the complex genetic relationships between the vegetative period, colour, size and production of seed, the composition (polysaccharides and proteins) and physico-chemical properties of soluble mucilages collected at 28 °C from seeds of 18 lines grown in St Petersburg area.
24852819	2	15	theme	mucilages	495:503	arg1	colour					368:373	colour	368:373	colour	368:373	The aim of the work was to investigate the complex genetic relationships between the vegetative period, colour, size and production of seed, the composition (polysaccharides and proteins) and physico-chemical properties of soluble mucilages collected at 28 °C from seeds of 18 lines grown in St Petersburg area.
24852819	3	16	theme	mucilages	674:682	arg1	seeds					654:658	seeds	654:658	seeds	654:658	The vegetative period duration was found to impact the size and production of seeds, the yield of mucilages, including the polysaccharides, and the galactosidase enzymes, as well as their composition (mainly the rhamnogalacturonan I moieties) and some of their properties (mainly viscosity).
24852819	3	16	theme	mucilages	674:682	arg1	yield					665:669	the yield	661:669	the yield of mucilages, including the polysaccharides, and the galactosidase enzymes, as well as their composition (mainly the rhamnogalacturonan I moieties)	661:817	The vegetative period duration was found to impact the size and production of seeds, the yield of mucilages, including the polysaccharides, and the galactosidase enzymes, as well as their composition (mainly the rhamnogalacturonan I moieties) and some of their properties (mainly viscosity).
24852819	5	17	theme	fibre	1188:1192	arg1	lines					1194:1198	Seven fibre lines	1182:1198	Seven fibre lines	1182:1198	Seven fibre lines had mucilages particularly rich in galactose.
24852819	8	18	with	lines	1640:1644	arg1	seeds					1657:1661	brown seeds	1651:1661	brown seeds	1651:1661	Main-component analyses of line characters showed a large diversity in linseeds mainly due to their different origin but small variation in Russian fibre lines with brown seeds.
24852819	8	19	theme	Russian	1626:1632	arg1	lines					1640:1644	Russian fibre lines	1626:1644	Russian fibre lines with brown seeds	1626:1661	Main-component analyses of line characters showed a large diversity in linseeds mainly due to their different origin but small variation in Russian fibre lines with brown seeds.
24852819	0	20	theme	genetic	84:90	arg1	collection					92:101	the flax genetic collection	75:101	the flax genetic collection of Vavilov Institute	75:122	Variability of seed traits and properties of soluble mucilages in lines of the flax genetic collection of Vavilov Institute.
24852819	4	21	theme	fibre	1149:1153	arg1	lines					1155:1159	4 fibre lines	1147:1159	4 fibre lines	1147:1159	Data allowed to significantly distinguish 6 fibre lines with mucilages enriched in rhamnogalacturonan I, 6 lines with mucilages enriched in arabinoxylan including 5 linseeds and 1 mutated fibre-line, and 5 lines with mucilages enriched in homogalacturonan-like polymer including 4 fibre lines and 1 brown linseed.
24852819	0	22	theme	collection	92:101	arg1	lines					66:70	lines	66:70	lines of the flax genetic collection of Vavilov Institute	66:122	Variability of seed traits and properties of soluble mucilages in lines of the flax genetic collection of Vavilov Institute.
24852819	3	23	theme	yield	665:669	arg1	production					640:649	production	640:649	production	640:649	The vegetative period duration was found to impact the size and production of seeds, the yield of mucilages, including the polysaccharides, and the galactosidase enzymes, as well as their composition (mainly the rhamnogalacturonan I moieties) and some of their properties (mainly viscosity).
24852819	3	23	theme	yield	665:669	arg1	size					631:634	size	631:634	size	631:634	The vegetative period duration was found to impact the size and production of seeds, the yield of mucilages, including the polysaccharides, and the galactosidase enzymes, as well as their composition (mainly the rhamnogalacturonan I moieties) and some of their properties (mainly viscosity).
24852819	5	24	from	rich	1227:1230	arg1	galactose					1235:1243	galactose	1235:1243	galactose	1235:1243	Seven fibre lines had mucilages particularly rich in galactose.
24852819	6	25	theme	High	1246:1249	arg1	variability					1264:1274	High to very high variability	1246:1274	High to very high variability	1246:1274	High to very high variability was found for 14 traits.
24852819	2	26	dep	composition	409:419	arg1	proteins					442:449	proteins	442:449	proteins	442:449	The aim of the work was to investigate the complex genetic relationships between the vegetative period, colour, size and production of seed, the composition (polysaccharides and proteins) and physico-chemical properties of soluble mucilages collected at 28 °C from seeds of 18 lines grown in St Petersburg area.
24852819	2	26	dep	composition	409:419	arg1	polysaccharides					422:436	polysaccharides	422:436	polysaccharides	422:436	The aim of the work was to investigate the complex genetic relationships between the vegetative period, colour, size and production of seed, the composition (polysaccharides and proteins) and physico-chemical properties of soluble mucilages collected at 28 °C from seeds of 18 lines grown in St Petersburg area.
24852819	8	27	from	variation	1613:1621	arg1	lines					1640:1644	Russian fibre lines	1626:1644	Russian fibre lines with brown seeds	1626:1661	Main-component analyses of line characters showed a large diversity in linseeds mainly due to their different origin but small variation in Russian fibre lines with brown seeds.
24852819	7	28	dep	characters	1324:1333	arg1	galactosidase					1360:1372	galactosidase	1360:1372	galactosidase	1360:1372	Relatively independent characters (form/shape, protein and galactosidase) were identified and could be combined by breeding, with a focus on mucilage yield, composition and properties.
24852819	7	28	dep	characters	1324:1333	arg1	protein					1348:1354	protein	1348:1354	protein	1348:1354	Relatively independent characters (form/shape, protein and galactosidase) were identified and could be combined by breeding, with a focus on mucilage yield, composition and properties.
24852819	7	28	dep	characters	1324:1333	arg1	form/shape					1336:1345	form/shape	1336:1345	form/shape	1336:1345	Relatively independent characters (form/shape, protein and galactosidase) were identified and could be combined by breeding, with a focus on mucilage yield, composition and properties.
24852819	7	28	dep	characters	1324:1333	arg1	characters					1324:1333	Relatively independent characters	1301:1333	Relatively independent characters (form/shape, protein and galactosidase)	1301:1373	Relatively independent characters (form/shape, protein and galactosidase) were identified and could be combined by breeding, with a focus on mucilage yield, composition and properties.
24852819	0	29	theme	traits	20:25	arg1	Variability					0:10	Variability	0:10	Variability of seed traits and properties of soluble mucilages in lines of the flax genetic collection of Vavilov Institute.	0:123	Variability of seed traits and properties of soluble mucilages in lines of the flax genetic collection of Vavilov Institute.
24852819	8	30	from	diversity	1544:1552	arg1	linseeds					1557:1564	linseeds	1557:1564	linseeds mainly due to their different origin	1557:1601	Main-component analyses of line characters showed a large diversity in linseeds mainly due to their different origin but small variation in Russian fibre lines with brown seeds.
24852819	8	31	theme	brown	1651:1655	arg1	seeds					1657:1661	brown seeds	1651:1661	brown seeds	1651:1661	Main-component analyses of line characters showed a large diversity in linseeds mainly due to their different origin but small variation in Russian fibre lines with brown seeds.
24852819	2	32	theme	seed	399:402	arg1	size					376:379	size	376:379	size	376:379	The aim of the work was to investigate the complex genetic relationships between the vegetative period, colour, size and production of seed, the composition (polysaccharides and proteins) and physico-chemical properties of soluble mucilages collected at 28 °C from seeds of 18 lines grown in St Petersburg area.
24852819	2	32	theme	seed	399:402	arg1	composition					409:419	the composition	405:419	the composition (polysaccharides and proteins)	405:450	The aim of the work was to investigate the complex genetic relationships between the vegetative period, colour, size and production of seed, the composition (polysaccharides and proteins) and physico-chemical properties of soluble mucilages collected at 28 °C from seeds of 18 lines grown in St Petersburg area.
24852819	2	32	theme	seed	399:402	arg1	period					360:365	the vegetative period	345:365	the vegetative period	345:365	The aim of the work was to investigate the complex genetic relationships between the vegetative period, colour, size and production of seed, the composition (polysaccharides and proteins) and physico-chemical properties of soluble mucilages collected at 28 °C from seeds of 18 lines grown in St Petersburg area.
24852819	2	32	theme	seed	399:402	arg1	properties					473:482	physico-chemical properties	456:482	physico-chemical properties	456:482	The aim of the work was to investigate the complex genetic relationships between the vegetative period, colour, size and production of seed, the composition (polysaccharides and proteins) and physico-chemical properties of soluble mucilages collected at 28 °C from seeds of 18 lines grown in St Petersburg area.
24852819	2	32	theme	seed	399:402	arg1	production					385:394	production	385:394	production	385:394	The aim of the work was to investigate the complex genetic relationships between the vegetative period, colour, size and production of seed, the composition (polysaccharides and proteins) and physico-chemical properties of soluble mucilages collected at 28 °C from seeds of 18 lines grown in St Petersburg area.
24852819	2	32	theme	seed	399:402	arg1	colour					368:373	colour	368:373	colour	368:373	The aim of the work was to investigate the complex genetic relationships between the vegetative period, colour, size and production of seed, the composition (polysaccharides and proteins) and physico-chemical properties of soluble mucilages collected at 28 °C from seeds of 18 lines grown in St Petersburg area.
24852819	0	33	theme	seed	15:18	arg1	traits					20:25	seed traits	15:25	seed traits	15:25	Variability of seed traits and properties of soluble mucilages in lines of the flax genetic collection of Vavilov Institute.
24852819	8	34	theme	Main-component	1486:1499	arg1	analyses					1501:1508	Main-component analyses	1486:1508	Main-component analyses of line characters	1486:1527	Main-component analyses of line characters showed a large diversity in linseeds mainly due to their different origin but small variation in Russian fibre lines with brown seeds.
24852819	2	35	theme	work	279:282	arg1	aim					268:270	The aim	264:270	The aim of the work	264:282	The aim of the work was to investigate the complex genetic relationships between the vegetative period, colour, size and production of seed, the composition (polysaccharides and proteins) and physico-chemical properties of soluble mucilages collected at 28 °C from seeds of 18 lines grown in St Petersburg area.
24852819	4	36	theme	mutated	1048:1054	arg1	fibre-line					1056:1065	1 mutated fibre-line	1046:1065	1 mutated fibre-line	1046:1065	Data allowed to significantly distinguish 6 fibre lines with mucilages enriched in rhamnogalacturonan I, 6 lines with mucilages enriched in arabinoxylan including 5 linseeds and 1 mutated fibre-line, and 5 lines with mucilages enriched in homogalacturonan-like polymer including 4 fibre lines and 1 brown linseed.
24852819	0	37	theme	properties	31:40	arg1	Variability					0:10	Variability	0:10	Variability of seed traits and properties of soluble mucilages in lines of the flax genetic collection of Vavilov Institute.	0:123	Variability of seed traits and properties of soluble mucilages in lines of the flax genetic collection of Vavilov Institute.
24852819	7	38	theme	independent	1312:1322	arg1	galactosidase					1360:1372	galactosidase	1360:1372	galactosidase	1360:1372	Relatively independent characters (form/shape, protein and galactosidase) were identified and could be combined by breeding, with a focus on mucilage yield, composition and properties.
24852819	7	38	theme	independent	1312:1322	arg1	protein					1348:1354	protein	1348:1354	protein	1348:1354	Relatively independent characters (form/shape, protein and galactosidase) were identified and could be combined by breeding, with a focus on mucilage yield, composition and properties.
24852819	7	38	theme	independent	1312:1322	arg1	form/shape					1336:1345	form/shape	1336:1345	form/shape	1336:1345	Relatively independent characters (form/shape, protein and galactosidase) were identified and could be combined by breeding, with a focus on mucilage yield, composition and properties.
24852819	7	38	theme	independent	1312:1322	arg1	characters					1324:1333	Relatively independent characters	1301:1333	Relatively independent characters (form/shape, protein and galactosidase)	1301:1373	Relatively independent characters (form/shape, protein and galactosidase) were identified and could be combined by breeding, with a focus on mucilage yield, composition and properties.
24852819	8	39	theme	small	1607:1611	arg1	variation					1613:1621	small variation	1607:1621	small variation in Russian fibre lines with brown seeds	1607:1661	Main-component analyses of line characters showed a large diversity in linseeds mainly due to their different origin but small variation in Russian fibre lines with brown seeds.
24852819	1	40	theme	physico-chemical	188:203	arg1	properties					205:214	physico-chemical properties	188:214	physico-chemical properties	188:214	Upon hydration, flax seeds secrete mucilages whose content and physico-chemical properties vary according to the genotype and environment.
24852819	4	41	with	lines	1074:1078	arg1	mucilages					1085:1093	mucilages	1085:1093	mucilages enriched in homogalacturonan-like polymer including 4 fibre lines and 1 brown linseed	1085:1179	Data allowed to significantly distinguish 6 fibre lines with mucilages enriched in rhamnogalacturonan I, 6 lines with mucilages enriched in arabinoxylan including 5 linseeds and 1 mutated fibre-line, and 5 lines with mucilages enriched in homogalacturonan-like polymer including 4 fibre lines and 1 brown linseed.
24852819	4	41	with	lines	1074:1078	arg1	mucilages					986:994	mucilages	986:994	mucilages enriched in arabinoxylan including 5 linseeds and 1 mutated fibre-line	986:1065	Data allowed to significantly distinguish 6 fibre lines with mucilages enriched in rhamnogalacturonan I, 6 lines with mucilages enriched in arabinoxylan including 5 linseeds and 1 mutated fibre-line, and 5 lines with mucilages enriched in homogalacturonan-like polymer including 4 fibre lines and 1 brown linseed.
24852819	4	41	with	lines	1074:1078	arg1	mucilages					929:937	mucilages	929:937	mucilages enriched in rhamnogalacturonan I	929:970	Data allowed to significantly distinguish 6 fibre lines with mucilages enriched in rhamnogalacturonan I, 6 lines with mucilages enriched in arabinoxylan including 5 linseeds and 1 mutated fibre-line, and 5 lines with mucilages enriched in homogalacturonan-like polymer including 4 fibre lines and 1 brown linseed.
24852819	0	42	theme	Institute	114:122	arg1	collection					92:101	the flax genetic collection	75:101	the flax genetic collection of Vavilov Institute	75:122	Variability of seed traits and properties of soluble mucilages in lines of the flax genetic collection of Vavilov Institute.
24852819	7	43	theme	mucilage	1442:1449	arg1	yield					1451:1455	mucilage yield	1442:1455	mucilage yield	1442:1455	Relatively independent characters (form/shape, protein and galactosidase) were identified and could be combined by breeding, with a focus on mucilage yield, composition and properties.
24852819	4	44	theme	homogalacturonan-like	1107:1127	arg1	polymer					1129:1135	homogalacturonan-like polymer	1107:1135	homogalacturonan-like polymer including 4 fibre lines and 1 brown linseed	1107:1179	Data allowed to significantly distinguish 6 fibre lines with mucilages enriched in rhamnogalacturonan I, 6 lines with mucilages enriched in arabinoxylan including 5 linseeds and 1 mutated fibre-line, and 5 lines with mucilages enriched in homogalacturonan-like polymer including 4 fibre lines and 1 brown linseed.
24852819	2	45	theme	soluble	487:493	arg1	mucilages					495:503	soluble mucilages	487:503	soluble mucilages collected at 28 °C from seeds of 18 lines grown in St Petersburg area	487:573	The aim of the work was to investigate the complex genetic relationships between the vegetative period, colour, size and production of seed, the composition (polysaccharides and proteins) and physico-chemical properties of soluble mucilages collected at 28 °C from seeds of 18 lines grown in St Petersburg area.
24852819	0	46	theme	soluble	45:51	arg1	mucilages					53:61	soluble mucilages	45:61	soluble mucilages	45:61	Variability of seed traits and properties of soluble mucilages in lines of the flax genetic collection of Vavilov Institute.
24852819	3	47	theme	galactosidase	724:736	arg1	enzymes					738:744	the galactosidase enzymes	720:744	the galactosidase enzymes	720:744	The vegetative period duration was found to impact the size and production of seeds, the yield of mucilages, including the polysaccharides, and the galactosidase enzymes, as well as their composition (mainly the rhamnogalacturonan I moieties) and some of their properties (mainly viscosity).
24852819	5	48	from	galactose	1235:1243	arg1	rich					1227:1230	rich	1227:1230	rich	1227:1230	Seven fibre lines had mucilages particularly rich in galactose.
24852819	8	49	theme	characters	1518:1527	arg1	analyses					1501:1508	Main-component analyses	1486:1508	Main-component analyses of line characters	1486:1527	Main-component analyses of line characters showed a large diversity in linseeds mainly due to their different origin but small variation in Russian fibre lines with brown seeds.
24852819	2	50	theme	lines	541:545	arg1	seeds					529:533	seeds	529:533	seeds of 18 lines grown in St Petersburg area	529:573	The aim of the work was to investigate the complex genetic relationships between the vegetative period, colour, size and production of seed, the composition (polysaccharides and proteins) and physico-chemical properties of soluble mucilages collected at 28 °C from seeds of 18 lines grown in St Petersburg area.
24852819	2	51	theme	genetic	315:321	arg1	relationships					323:335	the complex genetic relationships	303:335	the complex genetic relationships between the vegetative period, colour, size and production of seed, the composition (polysaccharides and proteins) and physico-chemical properties of soluble mucilages collected at 28 °C from seeds of 18 lines grown in St Petersburg area	303:573	The aim of the work was to investigate the complex genetic relationships between the vegetative period, colour, size and production of seed, the composition (polysaccharides and proteins) and physico-chemical properties of soluble mucilages collected at 28 °C from seeds of 18 lines grown in St Petersburg area.
24852819	3	52	theme	rhamnogalacturonan	788:805	arg1	moieties					809:816	the rhamnogalacturonan I moieties	784:816	mainly the rhamnogalacturonan I moieties	777:816	The vegetative period duration was found to impact the size and production of seeds, the yield of mucilages, including the polysaccharides, and the galactosidase enzymes, as well as their composition (mainly the rhamnogalacturonan I moieties) and some of their properties (mainly viscosity).
24852819	8	53	theme	line	1513:1516	arg1	characters					1518:1527	line characters	1513:1527	line characters	1513:1527	Main-component analyses of line characters showed a large diversity in linseeds mainly due to their different origin but small variation in Russian fibre lines with brown seeds.
24852819	4	54	with	lines	975:979	arg1	mucilages					986:994	mucilages	986:994	mucilages enriched in arabinoxylan including 5 linseeds and 1 mutated fibre-line	986:1065	Data allowed to significantly distinguish 6 fibre lines with mucilages enriched in rhamnogalacturonan I, 6 lines with mucilages enriched in arabinoxylan including 5 linseeds and 1 mutated fibre-line, and 5 lines with mucilages enriched in homogalacturonan-like polymer including 4 fibre lines and 1 brown linseed.
24852819	4	54	with	lines	975:979	arg1	mucilages					1085:1093	mucilages	1085:1093	mucilages enriched in homogalacturonan-like polymer including 4 fibre lines and 1 brown linseed	1085:1179	Data allowed to significantly distinguish 6 fibre lines with mucilages enriched in rhamnogalacturonan I, 6 lines with mucilages enriched in arabinoxylan including 5 linseeds and 1 mutated fibre-line, and 5 lines with mucilages enriched in homogalacturonan-like polymer including 4 fibre lines and 1 brown linseed.
24852819	4	54	with	lines	975:979	arg1	mucilages					929:937	mucilages	929:937	mucilages enriched in rhamnogalacturonan I	929:970	Data allowed to significantly distinguish 6 fibre lines with mucilages enriched in rhamnogalacturonan I, 6 lines with mucilages enriched in arabinoxylan including 5 linseeds and 1 mutated fibre-line, and 5 lines with mucilages enriched in homogalacturonan-like polymer including 4 fibre lines and 1 brown linseed.
24852819	3	55	theme	vegetative	580:589	arg1	duration					598:605	The vegetative period duration	576:605	The vegetative period duration	576:605	The vegetative period duration was found to impact the size and production of seeds, the yield of mucilages, including the polysaccharides, and the galactosidase enzymes, as well as their composition (mainly the rhamnogalacturonan I moieties) and some of their properties (mainly viscosity).
24852819	2	56	theme	complex	307:313	arg1	relationships					323:335	the complex genetic relationships	303:335	the complex genetic relationships between the vegetative period, colour, size and production of seed, the composition (polysaccharides and proteins) and physico-chemical properties of soluble mucilages collected at 28 °C from seeds of 18 lines grown in St Petersburg area	303:573	The aim of the work was to investigate the complex genetic relationships between the vegetative period, colour, size and production of seed, the composition (polysaccharides and proteins) and physico-chemical properties of soluble mucilages collected at 28 °C from seeds of 18 lines grown in St Petersburg area.
24852819	3	57	theme	I	807:807	arg1	moieties					809:816	the rhamnogalacturonan I moieties	784:816	mainly the rhamnogalacturonan I moieties	777:816	The vegetative period duration was found to impact the size and production of seeds, the yield of mucilages, including the polysaccharides, and the galactosidase enzymes, as well as their composition (mainly the rhamnogalacturonan I moieties) and some of their properties (mainly viscosity).
24852819	1	58	dep	genotype	238:245	arg1	the					234:236	the	234:236	the	234:236	Upon hydration, flax seeds secrete mucilages whose content and physico-chemical properties vary according to the genotype and environment.
24852819	3	59	theme	period	591:596	arg1	duration					598:605	The vegetative period duration	576:605	The vegetative period duration	576:605	The vegetative period duration was found to impact the size and production of seeds, the yield of mucilages, including the polysaccharides, and the galactosidase enzymes, as well as their composition (mainly the rhamnogalacturonan I moieties) and some of their properties (mainly viscosity).
24852819	5	60	theme	rich	1227:1230	arg1	mucilages					1204:1212	mucilages	1204:1212	mucilages particularly rich in galactose	1204:1243	Seven fibre lines had mucilages particularly rich in galactose.
24852819	3	61	dep	viscosity	856:864	arg1	mainly					849:854	mainly	849:854	mainly	849:854	The vegetative period duration was found to impact the size and production of seeds, the yield of mucilages, including the polysaccharides, and the galactosidase enzymes, as well as their composition (mainly the rhamnogalacturonan I moieties) and some of their properties (mainly viscosity).
24852819	8	62	theme	different	1586:1594	arg1	origin					1596:1601	their different origin	1580:1601	their different origin	1580:1601	Main-component analyses of line characters showed a large diversity in linseeds mainly due to their different origin but small variation in Russian fibre lines with brown seeds.
24852819	3	63	dep	found	611:615	arg1	viscosity					856:864	viscosity	856:864	viscosity	856:864	The vegetative period duration was found to impact the size and production of seeds, the yield of mucilages, including the polysaccharides, and the galactosidase enzymes, as well as their composition (mainly the rhamnogalacturonan I moieties) and some of their properties (mainly viscosity).
24852819	0	64	theme	mucilages	53:61	arg1	properties					31:40	properties	31:40	properties of soluble mucilages	31:61	Variability of seed traits and properties of soluble mucilages in lines of the flax genetic collection of Vavilov Institute.
24852819	0	64	theme	mucilages	53:61	arg1	traits					20:25	seed traits	15:25	seed traits	15:25	Variability of seed traits and properties of soluble mucilages in lines of the flax genetic collection of Vavilov Institute.
24852819	3	65	theme	seeds	654:658	arg1	production					640:649	production	640:649	production	640:649	The vegetative period duration was found to impact the size and production of seeds, the yield of mucilages, including the polysaccharides, and the galactosidase enzymes, as well as their composition (mainly the rhamnogalacturonan I moieties) and some of their properties (mainly viscosity).
24852819	3	65	theme	seeds	654:658	arg1	size					631:634	size	631:634	size	631:634	The vegetative period duration was found to impact the size and production of seeds, the yield of mucilages, including the polysaccharides, and the galactosidase enzymes, as well as their composition (mainly the rhamnogalacturonan I moieties) and some of their properties (mainly viscosity).
24852819	6	66	dep	High	1246:1249	arg1	high					1259:1262	high	1259:1262	high	1259:1262	High to very high variability was found for 14 traits.
24852819	6	66	dep	High	1246:1249	arg1	to					1251:1252	to	1251:1252	to	1251:1252	High to very high variability was found for 14 traits.
24852819	2	67	theme	vegetative	349:358	arg1	period					360:365	the vegetative period	345:365	the vegetative period	345:365	The aim of the work was to investigate the complex genetic relationships between the vegetative period, colour, size and production of seed, the composition (polysaccharides and proteins) and physico-chemical properties of soluble mucilages collected at 28 °C from seeds of 18 lines grown in St Petersburg area.
24852819	8	68	theme	due	1573:1575	arg1	linseeds					1557:1564	linseeds	1557:1564	linseeds mainly due to their different origin	1557:1601	Main-component analyses of line characters showed a large diversity in linseeds mainly due to their different origin but small variation in Russian fibre lines with brown seeds.
24852819	8	69	theme	large	1538:1542	arg1	diversity					1544:1552	a large diversity	1536:1552	a large diversity in linseeds mainly due to their different origin	1536:1601	Main-component analyses of line characters showed a large diversity in linseeds mainly due to their different origin but small variation in Russian fibre lines with brown seeds.
28422569	4	0	theme	potential	1006:1014	arg1	materials					1030:1038	potential antibacterial materials	1006:1038	potential antibacterial materials	1006:1038	And the results of inhibition zone test and antibacterial inhibition ratio indicated the cryogel/AgNPs composite exhibited prominent and durable antibacterial activity against Gram-negative E. coli and could be utilized as potential antibacterial materials.
28422569	1	1	theme	chitosan	185:192	arg1	cryogelation					141:152	cryogelation	141:152	cryogelation of methacrylated carboxymethyl chitosan (mCMC) and poly(ethylene glycol) diacrylate (PEGDA) precursors	141:255	Cryogel was synthesized through cryogelation of methacrylated carboxymethyl chitosan (mCMC) and poly(ethylene glycol) diacrylate (PEGDA) precursors by photopolymerization.
28422569	4	2	theme	inhibition	841:850	arg1	ratio					852:856	antibacterial inhibition ratio	827:856	antibacterial inhibition ratio	827:856	And the results of inhibition zone test and antibacterial inhibition ratio indicated the cryogel/AgNPs composite exhibited prominent and durable antibacterial activity against Gram-negative E. coli and could be utilized as potential antibacterial materials.
28422569	2	3	theme	inter-connective	346:361	arg1	behavior					336:343	fast swelling behavior	322:343	fast swelling behavior	322:343	Due to its excellent properties, such as fast swelling behavior, inter-connective porous structure, high water absorbing capacity, especially the presence of abundant carboxylmethyl groups on its backbone, the cryogel not only favored the absorption of silver ions but also was proved to be a good matrix for the incorporation of silver nanoparticles (AgNPs) by in situ chemical reduction.
28422569	2	3	theme	inter-connective	346:361	arg1	structure					370:378	inter-connective porous structure	346:378	inter-connective porous structure	346:378	Due to its excellent properties, such as fast swelling behavior, inter-connective porous structure, high water absorbing capacity, especially the presence of abundant carboxylmethyl groups on its backbone, the cryogel not only favored the absorption of silver ions but also was proved to be a good matrix for the incorporation of silver nanoparticles (AgNPs) by in situ chemical reduction.
28422569	2	4	theme	good	574:577	arg1	matrix					579:584	a good matrix	572:584	a good matrix	572:584	Due to its excellent properties, such as fast swelling behavior, inter-connective porous structure, high water absorbing capacity, especially the presence of abundant carboxylmethyl groups on its backbone, the cryogel not only favored the absorption of silver ions but also was proved to be a good matrix for the incorporation of silver nanoparticles (AgNPs) by in situ chemical reduction.
28422569	2	4	theme	good	574:577	arg1	cryogel					491:497	the cryogel	487:497	the cryogel	487:497	Due to its excellent properties, such as fast swelling behavior, inter-connective porous structure, high water absorbing capacity, especially the presence of abundant carboxylmethyl groups on its backbone, the cryogel not only favored the absorption of silver ions but also was proved to be a good matrix for the incorporation of silver nanoparticles (AgNPs) by in situ chemical reduction.
28422569	2	5	theme	excellent	292:300	arg1	behavior					336:343	fast swelling behavior	322:343	fast swelling behavior	322:343	Due to its excellent properties, such as fast swelling behavior, inter-connective porous structure, high water absorbing capacity, especially the presence of abundant carboxylmethyl groups on its backbone, the cryogel not only favored the absorption of silver ions but also was proved to be a good matrix for the incorporation of silver nanoparticles (AgNPs) by in situ chemical reduction.
28422569	2	5	theme	excellent	292:300	arg1	properties					302:311	its excellent properties	288:311	its excellent properties	288:311	Due to its excellent properties, such as fast swelling behavior, inter-connective porous structure, high water absorbing capacity, especially the presence of abundant carboxylmethyl groups on its backbone, the cryogel not only favored the absorption of silver ions but also was proved to be a good matrix for the incorporation of silver nanoparticles (AgNPs) by in situ chemical reduction.
28422569	3	6	dep	cryogel	727:733	arg1	the					723:725	the	723:725	the	723:725	The structure, morphology, and swelling behavior of the cryogel and cryogel/AgNPs composite were characterized.
28422569	2	7	theme	porous	363:368	arg1	behavior					336:343	fast swelling behavior	322:343	fast swelling behavior	322:343	Due to its excellent properties, such as fast swelling behavior, inter-connective porous structure, high water absorbing capacity, especially the presence of abundant carboxylmethyl groups on its backbone, the cryogel not only favored the absorption of silver ions but also was proved to be a good matrix for the incorporation of silver nanoparticles (AgNPs) by in situ chemical reduction.
28422569	2	7	theme	porous	363:368	arg1	structure					370:378	inter-connective porous structure	346:378	inter-connective porous structure	346:378	Due to its excellent properties, such as fast swelling behavior, inter-connective porous structure, high water absorbing capacity, especially the presence of abundant carboxylmethyl groups on its backbone, the cryogel not only favored the absorption of silver ions but also was proved to be a good matrix for the incorporation of silver nanoparticles (AgNPs) by in situ chemical reduction.
28422569	2	8	theme	silver	611:616	arg1	AgNPs					633:637	AgNPs	633:637	AgNPs	633:637	Due to its excellent properties, such as fast swelling behavior, inter-connective porous structure, high water absorbing capacity, especially the presence of abundant carboxylmethyl groups on its backbone, the cryogel not only favored the absorption of silver ions but also was proved to be a good matrix for the incorporation of silver nanoparticles (AgNPs) by in situ chemical reduction.
28422569	2	8	theme	silver	611:616	arg1	nanoparticles					618:630	silver nanoparticles	611:630	silver nanoparticles (AgNPs)	611:638	Due to its excellent properties, such as fast swelling behavior, inter-connective porous structure, high water absorbing capacity, especially the presence of abundant carboxylmethyl groups on its backbone, the cryogel not only favored the absorption of silver ions but also was proved to be a good matrix for the incorporation of silver nanoparticles (AgNPs) by in situ chemical reduction.
28422569	2	9	theme	nanoparticles	618:630	arg1	incorporation					594:606	the incorporation	590:606	the incorporation of silver nanoparticles (AgNPs) by in situ chemical reduction	590:668	Due to its excellent properties, such as fast swelling behavior, inter-connective porous structure, high water absorbing capacity, especially the presence of abundant carboxylmethyl groups on its backbone, the cryogel not only favored the absorption of silver ions but also was proved to be a good matrix for the incorporation of silver nanoparticles (AgNPs) by in situ chemical reduction.
28422569	4	10	theme	antibacterial	1016:1028	arg1	materials					1030:1038	potential antibacterial materials	1006:1038	potential antibacterial materials	1006:1038	And the results of inhibition zone test and antibacterial inhibition ratio indicated the cryogel/AgNPs composite exhibited prominent and durable antibacterial activity against Gram-negative E. coli and could be utilized as potential antibacterial materials.
28422569	4	11	theme	zone	813:816	arg1	test					818:821	inhibition zone test	802:821	inhibition zone test	802:821	And the results of inhibition zone test and antibacterial inhibition ratio indicated the cryogel/AgNPs composite exhibited prominent and durable antibacterial activity against Gram-negative E. coli and could be utilized as potential antibacterial materials.
28422569	2	12	theme	chemical	651:658	arg1	reduction					660:668	in situ chemical reduction	643:668	in situ chemical reduction	643:668	Due to its excellent properties, such as fast swelling behavior, inter-connective porous structure, high water absorbing capacity, especially the presence of abundant carboxylmethyl groups on its backbone, the cryogel not only favored the absorption of silver ions but also was proved to be a good matrix for the incorporation of silver nanoparticles (AgNPs) by in situ chemical reduction.
28422569	2	13	theme	absorbing	392:400	arg1	behavior					336:343	fast swelling behavior	322:343	fast swelling behavior	322:343	Due to its excellent properties, such as fast swelling behavior, inter-connective porous structure, high water absorbing capacity, especially the presence of abundant carboxylmethyl groups on its backbone, the cryogel not only favored the absorption of silver ions but also was proved to be a good matrix for the incorporation of silver nanoparticles (AgNPs) by in situ chemical reduction.
28422569	2	13	theme	absorbing	392:400	arg1	capacity					402:409	high water absorbing capacity	381:409	high water absorbing capacity	381:409	Due to its excellent properties, such as fast swelling behavior, inter-connective porous structure, high water absorbing capacity, especially the presence of abundant carboxylmethyl groups on its backbone, the cryogel not only favored the absorption of silver ions but also was proved to be a good matrix for the incorporation of silver nanoparticles (AgNPs) by in situ chemical reduction.
28422569	2	14	theme	groups	463:468	arg1	behavior					336:343	fast swelling behavior	322:343	fast swelling behavior	322:343	Due to its excellent properties, such as fast swelling behavior, inter-connective porous structure, high water absorbing capacity, especially the presence of abundant carboxylmethyl groups on its backbone, the cryogel not only favored the absorption of silver ions but also was proved to be a good matrix for the incorporation of silver nanoparticles (AgNPs) by in situ chemical reduction.
28422569	2	14	theme	groups	463:468	arg1	presence					427:434	the presence	423:434	the presence of abundant carboxylmethyl groups on its backbone	423:484	Due to its excellent properties, such as fast swelling behavior, inter-connective porous structure, high water absorbing capacity, especially the presence of abundant carboxylmethyl groups on its backbone, the cryogel not only favored the absorption of silver ions but also was proved to be a good matrix for the incorporation of silver nanoparticles (AgNPs) by in situ chemical reduction.
28422569	4	15	theme	antibacterial	827:839	arg1	ratio					852:856	antibacterial inhibition ratio	827:856	antibacterial inhibition ratio	827:856	And the results of inhibition zone test and antibacterial inhibition ratio indicated the cryogel/AgNPs composite exhibited prominent and durable antibacterial activity against Gram-negative E. coli and could be utilized as potential antibacterial materials.
28422569	1	16	dep	chitosan	185:192	arg1	precursors					246:255	diacrylate (PEGDA) precursors	227:255	diacrylate (PEGDA) precursors	227:255	Cryogel was synthesized through cryogelation of methacrylated carboxymethyl chitosan (mCMC) and poly(ethylene glycol) diacrylate (PEGDA) precursors by photopolymerization.
28422569	2	17	theme	water	386:390	arg1	behavior					336:343	fast swelling behavior	322:343	fast swelling behavior	322:343	Due to its excellent properties, such as fast swelling behavior, inter-connective porous structure, high water absorbing capacity, especially the presence of abundant carboxylmethyl groups on its backbone, the cryogel not only favored the absorption of silver ions but also was proved to be a good matrix for the incorporation of silver nanoparticles (AgNPs) by in situ chemical reduction.
28422569	2	17	theme	water	386:390	arg1	capacity					402:409	high water absorbing capacity	381:409	high water absorbing capacity	381:409	Due to its excellent properties, such as fast swelling behavior, inter-connective porous structure, high water absorbing capacity, especially the presence of abundant carboxylmethyl groups on its backbone, the cryogel not only favored the absorption of silver ions but also was proved to be a good matrix for the incorporation of silver nanoparticles (AgNPs) by in situ chemical reduction.
28422569	2	18	theme	carboxylmethyl	448:461	arg1	groups					463:468	abundant carboxylmethyl groups	439:468	abundant carboxylmethyl groups	439:468	Due to its excellent properties, such as fast swelling behavior, inter-connective porous structure, high water absorbing capacity, especially the presence of abundant carboxylmethyl groups on its backbone, the cryogel not only favored the absorption of silver ions but also was proved to be a good matrix for the incorporation of silver nanoparticles (AgNPs) by in situ chemical reduction.
28422569	2	19	theme	in	643:644	arg1	reduction					660:668	in situ chemical reduction	643:668	in situ chemical reduction	643:668	Due to its excellent properties, such as fast swelling behavior, inter-connective porous structure, high water absorbing capacity, especially the presence of abundant carboxylmethyl groups on its backbone, the cryogel not only favored the absorption of silver ions but also was proved to be a good matrix for the incorporation of silver nanoparticles (AgNPs) by in situ chemical reduction.
28422569	4	20	theme	test	818:821	arg1	results					791:797	the results	787:797	the results of inhibition zone test	787:821	And the results of inhibition zone test and antibacterial inhibition ratio indicated the cryogel/AgNPs composite exhibited prominent and durable antibacterial activity against Gram-negative E. coli and could be utilized as potential antibacterial materials.
28422569	4	20	theme	test	818:821	arg1	ratio					852:856	antibacterial inhibition ratio	827:856	antibacterial inhibition ratio	827:856	And the results of inhibition zone test and antibacterial inhibition ratio indicated the cryogel/AgNPs composite exhibited prominent and durable antibacterial activity against Gram-negative E. coli and could be utilized as potential antibacterial materials.
28422569	1	21	theme	poly	205:208	arg1	cryogelation					141:152	cryogelation	141:152	cryogelation of methacrylated carboxymethyl chitosan (mCMC) and poly(ethylene glycol) diacrylate (PEGDA) precursors	141:255	Cryogel was synthesized through cryogelation of methacrylated carboxymethyl chitosan (mCMC) and poly(ethylene glycol) diacrylate (PEGDA) precursors by photopolymerization.
28422569	0	22	theme	novel	17:21	arg1	chitosan-poly					37:49	a novel antibacterial chitosan-poly	15:49	a novel antibacterial chitosan-poly(ethylene glycol)	15:66	Preparation of a novel antibacterial chitosan-poly(ethylene glycol) cryogel/silver nanoparticles composites.
28422569	0	22	theme	novel	17:21	arg1	glycol					60:65	ethylene glycol	51:65	ethylene glycol	51:65	Preparation of a novel antibacterial chitosan-poly(ethylene glycol) cryogel/silver nanoparticles composites.
28422569	4	23	theme	durable	920:926	arg1	activity					942:949	prominent and durable antibacterial activity	906:949	prominent and durable antibacterial activity against Gram-negative E. coli	906:979	And the results of inhibition zone test and antibacterial inhibition ratio indicated the cryogel/AgNPs composite exhibited prominent and durable antibacterial activity against Gram-negative E. coli and could be utilized as potential antibacterial materials.
28422569	3	24	theme	swelling	702:709	arg1	behavior					711:718	swelling behavior	702:718	swelling behavior	702:718	The structure, morphology, and swelling behavior of the cryogel and cryogel/AgNPs composite were characterized.
28422569	1	25	theme	ethylene	210:217	arg1	poly					205:208	poly	205:208	poly(ethylene glycol)	205:225	Cryogel was synthesized through cryogelation of methacrylated carboxymethyl chitosan (mCMC) and poly(ethylene glycol) diacrylate (PEGDA) precursors by photopolymerization.
28422569	1	25	theme	ethylene	210:217	arg1	glycol					219:224	ethylene glycol	210:224	ethylene glycol	210:224	Cryogel was synthesized through cryogelation of methacrylated carboxymethyl chitosan (mCMC) and poly(ethylene glycol) diacrylate (PEGDA) precursors by photopolymerization.
28422569	0	26	theme	chitosan-poly	37:49	arg1	Preparation					0:10	Preparation	0:10	Preparation of a novel antibacterial chitosan-poly(ethylene glycol)	0:66	Preparation of a novel antibacterial chitosan-poly(ethylene glycol) cryogel/silver nanoparticles composites.
28422569	3	27	dep	structure	675:683	arg1	The					671:673	The	671:673	The	671:673	The structure, morphology, and swelling behavior of the cryogel and cryogel/AgNPs composite were characterized.
28422569	2	28	theme	ions	541:544	arg1	absorption					520:529	the absorption	516:529	the absorption of silver ions	516:544	Due to its excellent properties, such as fast swelling behavior, inter-connective porous structure, high water absorbing capacity, especially the presence of abundant carboxylmethyl groups on its backbone, the cryogel not only favored the absorption of silver ions but also was proved to be a good matrix for the incorporation of silver nanoparticles (AgNPs) by in situ chemical reduction.
28422569	2	29	attach	presence	427:434	arg1	backbone					477:484	its backbone	473:484	its backbone	473:484	Due to its excellent properties, such as fast swelling behavior, inter-connective porous structure, high water absorbing capacity, especially the presence of abundant carboxylmethyl groups on its backbone, the cryogel not only favored the absorption of silver ions but also was proved to be a good matrix for the incorporation of silver nanoparticles (AgNPs) by in situ chemical reduction.
28422569	2	29	attach	presence	427:434	arg2	groups					463:468	abundant carboxylmethyl groups	439:468	abundant carboxylmethyl groups	439:468	Due to its excellent properties, such as fast swelling behavior, inter-connective porous structure, high water absorbing capacity, especially the presence of abundant carboxylmethyl groups on its backbone, the cryogel not only favored the absorption of silver ions but also was proved to be a good matrix for the incorporation of silver nanoparticles (AgNPs) by in situ chemical reduction.
28422569	2	30	theme	high	381:384	arg1	behavior					336:343	fast swelling behavior	322:343	fast swelling behavior	322:343	Due to its excellent properties, such as fast swelling behavior, inter-connective porous structure, high water absorbing capacity, especially the presence of abundant carboxylmethyl groups on its backbone, the cryogel not only favored the absorption of silver ions but also was proved to be a good matrix for the incorporation of silver nanoparticles (AgNPs) by in situ chemical reduction.
28422569	2	30	theme	high	381:384	arg1	capacity					402:409	high water absorbing capacity	381:409	high water absorbing capacity	381:409	Due to its excellent properties, such as fast swelling behavior, inter-connective porous structure, high water absorbing capacity, especially the presence of abundant carboxylmethyl groups on its backbone, the cryogel not only favored the absorption of silver ions but also was proved to be a good matrix for the incorporation of silver nanoparticles (AgNPs) by in situ chemical reduction.
28422569	0	31	theme	antibacterial	23:35	arg1	chitosan-poly					37:49	a novel antibacterial chitosan-poly	15:49	a novel antibacterial chitosan-poly(ethylene glycol)	15:66	Preparation of a novel antibacterial chitosan-poly(ethylene glycol) cryogel/silver nanoparticles composites.
28422569	0	31	theme	antibacterial	23:35	arg1	glycol					60:65	ethylene glycol	51:65	ethylene glycol	51:65	Preparation of a novel antibacterial chitosan-poly(ethylene glycol) cryogel/silver nanoparticles composites.
28422569	3	32	theme	cryogel/AgNPs	739:751	arg1	composite					753:761	cryogel/AgNPs composite	739:761	cryogel/AgNPs composite	739:761	The structure, morphology, and swelling behavior of the cryogel and cryogel/AgNPs composite were characterized.
28422569	2	33	theme	silver	534:539	arg1	ions					541:544	silver ions	534:544	silver ions	534:544	Due to its excellent properties, such as fast swelling behavior, inter-connective porous structure, high water absorbing capacity, especially the presence of abundant carboxylmethyl groups on its backbone, the cryogel not only favored the absorption of silver ions but also was proved to be a good matrix for the incorporation of silver nanoparticles (AgNPs) by in situ chemical reduction.
28422569	2	34	theme	abundant	439:446	arg1	groups					463:468	abundant carboxylmethyl groups	439:468	abundant carboxylmethyl groups	439:468	Due to its excellent properties, such as fast swelling behavior, inter-connective porous structure, high water absorbing capacity, especially the presence of abundant carboxylmethyl groups on its backbone, the cryogel not only favored the absorption of silver ions but also was proved to be a good matrix for the incorporation of silver nanoparticles (AgNPs) by in situ chemical reduction.
28422569	0	35	theme	ethylene	51:58	arg1	chitosan-poly					37:49	a novel antibacterial chitosan-poly	15:49	a novel antibacterial chitosan-poly(ethylene glycol)	15:66	Preparation of a novel antibacterial chitosan-poly(ethylene glycol) cryogel/silver nanoparticles composites.
28422569	0	35	theme	ethylene	51:58	arg1	glycol					60:65	ethylene glycol	51:65	ethylene glycol	51:65	Preparation of a novel antibacterial chitosan-poly(ethylene glycol) cryogel/silver nanoparticles composites.
28422569	3	36	theme	composite	753:761	arg1	morphology					686:695	morphology	686:695	morphology	686:695	The structure, morphology, and swelling behavior of the cryogel and cryogel/AgNPs composite were characterized.
28422569	3	36	theme	composite	753:761	arg1	structure					675:683	structure	675:683	structure	675:683	The structure, morphology, and swelling behavior of the cryogel and cryogel/AgNPs composite were characterized.
28422569	3	36	theme	composite	753:761	arg1	behavior					711:718	swelling behavior	702:718	swelling behavior	702:718	The structure, morphology, and swelling behavior of the cryogel and cryogel/AgNPs composite were characterized.
28422569	4	37	theme	inhibition	802:811	arg1	test					818:821	inhibition zone test	802:821	inhibition zone test	802:821	And the results of inhibition zone test and antibacterial inhibition ratio indicated the cryogel/AgNPs composite exhibited prominent and durable antibacterial activity against Gram-negative E. coli and could be utilized as potential antibacterial materials.
28422569	4	38	theme	prominent	906:914	arg1	activity					942:949	prominent and durable antibacterial activity	906:949	prominent and durable antibacterial activity against Gram-negative E. coli	906:979	And the results of inhibition zone test and antibacterial inhibition ratio indicated the cryogel/AgNPs composite exhibited prominent and durable antibacterial activity against Gram-negative E. coli and could be utilized as potential antibacterial materials.
28422569	4	39	theme	antibacterial	928:940	arg1	activity					942:949	prominent and durable antibacterial activity	906:949	prominent and durable antibacterial activity against Gram-negative E. coli	906:979	And the results of inhibition zone test and antibacterial inhibition ratio indicated the cryogel/AgNPs composite exhibited prominent and durable antibacterial activity against Gram-negative E. coli and could be utilized as potential antibacterial materials.
28422569	3	40	theme	cryogel	727:733	arg1	morphology					686:695	morphology	686:695	morphology	686:695	The structure, morphology, and swelling behavior of the cryogel and cryogel/AgNPs composite were characterized.
28422569	3	40	theme	cryogel	727:733	arg1	structure					675:683	structure	675:683	structure	675:683	The structure, morphology, and swelling behavior of the cryogel and cryogel/AgNPs composite were characterized.
28422569	3	40	theme	cryogel	727:733	arg1	behavior					711:718	swelling behavior	702:718	swelling behavior	702:718	The structure, morphology, and swelling behavior of the cryogel and cryogel/AgNPs composite were characterized.
28422569	4	41	theme	cryogel/AgNPs	872:884	arg1	composite					886:894	the cryogel/AgNPs composite	868:894	the cryogel/AgNPs composite	868:894	And the results of inhibition zone test and antibacterial inhibition ratio indicated the cryogel/AgNPs composite exhibited prominent and durable antibacterial activity against Gram-negative E. coli and could be utilized as potential antibacterial materials.
28422569	2	42	dep	in	643:644	arg1	situ					646:649	situ	646:649	situ	646:649	Due to its excellent properties, such as fast swelling behavior, inter-connective porous structure, high water absorbing capacity, especially the presence of abundant carboxylmethyl groups on its backbone, the cryogel not only favored the absorption of silver ions but also was proved to be a good matrix for the incorporation of silver nanoparticles (AgNPs) by in situ chemical reduction.
28422569	2	43	dep	only	503:506	arg1	not					499:501	not	499:501	not	499:501	Due to its excellent properties, such as fast swelling behavior, inter-connective porous structure, high water absorbing capacity, especially the presence of abundant carboxylmethyl groups on its backbone, the cryogel not only favored the absorption of silver ions but also was proved to be a good matrix for the incorporation of silver nanoparticles (AgNPs) by in situ chemical reduction.
28422569	2	44	theme	swelling	327:334	arg1	behavior					336:343	fast swelling behavior	322:343	fast swelling behavior	322:343	Due to its excellent properties, such as fast swelling behavior, inter-connective porous structure, high water absorbing capacity, especially the presence of abundant carboxylmethyl groups on its backbone, the cryogel not only favored the absorption of silver ions but also was proved to be a good matrix for the incorporation of silver nanoparticles (AgNPs) by in situ chemical reduction.
28422569	2	44	theme	swelling	327:334	arg1	capacity					402:409	high water absorbing capacity	381:409	high water absorbing capacity	381:409	Due to its excellent properties, such as fast swelling behavior, inter-connective porous structure, high water absorbing capacity, especially the presence of abundant carboxylmethyl groups on its backbone, the cryogel not only favored the absorption of silver ions but also was proved to be a good matrix for the incorporation of silver nanoparticles (AgNPs) by in situ chemical reduction.
28422569	2	44	theme	swelling	327:334	arg1	structure					370:378	inter-connective porous structure	346:378	inter-connective porous structure	346:378	Due to its excellent properties, such as fast swelling behavior, inter-connective porous structure, high water absorbing capacity, especially the presence of abundant carboxylmethyl groups on its backbone, the cryogel not only favored the absorption of silver ions but also was proved to be a good matrix for the incorporation of silver nanoparticles (AgNPs) by in situ chemical reduction.
28422569	2	44	theme	swelling	327:334	arg1	presence					427:434	the presence	423:434	the presence of abundant carboxylmethyl groups on its backbone	423:484	Due to its excellent properties, such as fast swelling behavior, inter-connective porous structure, high water absorbing capacity, especially the presence of abundant carboxylmethyl groups on its backbone, the cryogel not only favored the absorption of silver ions but also was proved to be a good matrix for the incorporation of silver nanoparticles (AgNPs) by in situ chemical reduction.
28422569	1	45	theme	diacrylate	227:236	arg1	precursors					246:255	diacrylate (PEGDA) precursors	227:255	diacrylate (PEGDA) precursors	227:255	Cryogel was synthesized through cryogelation of methacrylated carboxymethyl chitosan (mCMC) and poly(ethylene glycol) diacrylate (PEGDA) precursors by photopolymerization.
28422569	4	46	dep	indicated	858:866	arg1	exhibited					896:904	exhibited	896:904	exhibited prominent and durable antibacterial activity against Gram-negative E. coli	896:979	And the results of inhibition zone test and antibacterial inhibition ratio indicated the cryogel/AgNPs composite exhibited prominent and durable antibacterial activity against Gram-negative E. coli and could be utilized as potential antibacterial materials.
28422569	4	46	dep	indicated	858:866	arg1	utilized					994:1001	utilized	994:1001	could be utilized as potential antibacterial materials	985:1038	And the results of inhibition zone test and antibacterial inhibition ratio indicated the cryogel/AgNPs composite exhibited prominent and durable antibacterial activity against Gram-negative E. coli and could be utilized as potential antibacterial materials.
28422569	2	47	theme	fast	322:325	arg1	behavior					336:343	fast swelling behavior	322:343	fast swelling behavior	322:343	Due to its excellent properties, such as fast swelling behavior, inter-connective porous structure, high water absorbing capacity, especially the presence of abundant carboxylmethyl groups on its backbone, the cryogel not only favored the absorption of silver ions but also was proved to be a good matrix for the incorporation of silver nanoparticles (AgNPs) by in situ chemical reduction.
28422569	2	47	theme	fast	322:325	arg1	capacity					402:409	high water absorbing capacity	381:409	high water absorbing capacity	381:409	Due to its excellent properties, such as fast swelling behavior, inter-connective porous structure, high water absorbing capacity, especially the presence of abundant carboxylmethyl groups on its backbone, the cryogel not only favored the absorption of silver ions but also was proved to be a good matrix for the incorporation of silver nanoparticles (AgNPs) by in situ chemical reduction.
28422569	2	47	theme	fast	322:325	arg1	structure					370:378	inter-connective porous structure	346:378	inter-connective porous structure	346:378	Due to its excellent properties, such as fast swelling behavior, inter-connective porous structure, high water absorbing capacity, especially the presence of abundant carboxylmethyl groups on its backbone, the cryogel not only favored the absorption of silver ions but also was proved to be a good matrix for the incorporation of silver nanoparticles (AgNPs) by in situ chemical reduction.
28422569	2	47	theme	fast	322:325	arg1	presence					427:434	the presence	423:434	the presence of abundant carboxylmethyl groups on its backbone	423:484	Due to its excellent properties, such as fast swelling behavior, inter-connective porous structure, high water absorbing capacity, especially the presence of abundant carboxylmethyl groups on its backbone, the cryogel not only favored the absorption of silver ions but also was proved to be a good matrix for the incorporation of silver nanoparticles (AgNPs) by in situ chemical reduction.
28422569	1	48	theme	methacrylated	157:169	arg1	mCMC					195:198	mCMC	195:198	mCMC	195:198	Cryogel was synthesized through cryogelation of methacrylated carboxymethyl chitosan (mCMC) and poly(ethylene glycol) diacrylate (PEGDA) precursors by photopolymerization.
28422569	1	48	theme	methacrylated	157:169	arg1	chitosan					185:192	methacrylated carboxymethyl chitosan	157:192	methacrylated carboxymethyl chitosan (mCMC)	157:199	Cryogel was synthesized through cryogelation of methacrylated carboxymethyl chitosan (mCMC) and poly(ethylene glycol) diacrylate (PEGDA) precursors by photopolymerization.
28422569	1	49	theme	PEGDA	239:243	arg1	precursors					246:255	diacrylate (PEGDA) precursors	227:255	diacrylate (PEGDA) precursors	227:255	Cryogel was synthesized through cryogelation of methacrylated carboxymethyl chitosan (mCMC) and poly(ethylene glycol) diacrylate (PEGDA) precursors by photopolymerization.
28422569	2	50	from	presence	427:434	arg1	backbone					477:484	its backbone	473:484	its backbone	473:484	Due to its excellent properties, such as fast swelling behavior, inter-connective porous structure, high water absorbing capacity, especially the presence of abundant carboxylmethyl groups on its backbone, the cryogel not only favored the absorption of silver ions but also was proved to be a good matrix for the incorporation of silver nanoparticles (AgNPs) by in situ chemical reduction.
28422569	1	51	theme	carboxymethyl	171:183	arg1	mCMC					195:198	mCMC	195:198	mCMC	195:198	Cryogel was synthesized through cryogelation of methacrylated carboxymethyl chitosan (mCMC) and poly(ethylene glycol) diacrylate (PEGDA) precursors by photopolymerization.
28422569	1	51	theme	carboxymethyl	171:183	arg1	chitosan					185:192	methacrylated carboxymethyl chitosan	157:192	methacrylated carboxymethyl chitosan (mCMC)	157:199	Cryogel was synthesized through cryogelation of methacrylated carboxymethyl chitosan (mCMC) and poly(ethylene glycol) diacrylate (PEGDA) precursors by photopolymerization.
25965461	5	0	dep	→	1002:1002	arg1	-β-D-Glcp-					989:998	-β-D-Glcp-	989:998	-β-D-Glcp-	989:998	The main chain of ABPS-21 was made up of → 4)-β-D-Galp-(1 → 4)-β-D-Glcp-(1 → 4)-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 2)-α-L-Rhap-(1 →, and two side chains α-l-Rhap-(1 → 4)-β-D-Glcp-(1 → and α-D-galpA-(1 → were attached to the backbone chain at O-6 position of 1,4,6-linked β-D-Glcp and 1,4,6-linked β-D-Galp, respectively.
25965461	5	1	dep	-β-D-Galp-	903:912	arg1	4					901:901	4	901:901	4	901:901	The main chain of ABPS-21 was made up of → 4)-β-D-Galp-(1 → 4)-β-D-Glcp-(1 → 4)-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 2)-α-L-Rhap-(1 →, and two side chains α-l-Rhap-(1 → 4)-β-D-Glcp-(1 → and α-D-galpA-(1 → were attached to the backbone chain at O-6 position of 1,4,6-linked β-D-Glcp and 1,4,6-linked β-D-Galp, respectively.
25965461	6	2	theme	branched	1160:1167	arg1	ABPS-21					1141:1147	ABPS-21	1141:1147	ABPS-21	1141:1147	ABPS-21, an acidic branched polysaccharide from A. brachypus, was not described up to now.
25965461	6	2	theme	branched	1160:1167	arg1	polysaccharide					1169:1182	an acidic branched polysaccharide	1150:1182	an acidic branched polysaccharide from A. brachypus	1150:1200	ABPS-21, an acidic branched polysaccharide from A. brachypus, was not described up to now.
25965461	4	3	theme	3.0:2.0:2.0:1.0	741:755	arg1	ratio					732:736	a molar ratio	724:736	a molar ratio of 3.0:2.0:2.0:1.0, respectively	724:769	The result showed that ABPS-21 was a homogeneous heteropolysaccharide including galactose, glucose, rhamnose and galacturonic acid in a molar ratio of 3.0:2.0:2.0:1.0, respectively, with an average molecular weight of 1.06 × 10(5)Da.
25965461	5	4	theme	1,4,6-linked	1078:1089	arg1	β-D-Glcp					1091:1098	1,4,6-linked β-D-Glcp	1078:1098	1,4,6-linked β-D-Glcp	1078:1098	The main chain of ABPS-21 was made up of → 4)-β-D-Galp-(1 → 4)-β-D-Glcp-(1 → 4)-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 2)-α-L-Rhap-(1 →, and two side chains α-l-Rhap-(1 → 4)-β-D-Glcp-(1 → and α-D-galpA-(1 → were attached to the backbone chain at O-6 position of 1,4,6-linked β-D-Glcp and 1,4,6-linked β-D-Galp, respectively.
25965461	4	5	from	heteropolysaccharide	639:658	arg1	ratio					732:736	a molar ratio	724:736	a molar ratio of 3.0:2.0:2.0:1.0, respectively	724:769	The result showed that ABPS-21 was a homogeneous heteropolysaccharide including galactose, glucose, rhamnose and galacturonic acid in a molar ratio of 3.0:2.0:2.0:1.0, respectively, with an average molecular weight of 1.06 × 10(5)Da.
25965461	5	6	theme	β-D-Glcp	1091:1098	arg1	position					1066:1073	O-6 position	1062:1073	O-6 position of 1,4,6-linked β-D-Glcp and 1,4,6-linked β-D-Galp	1062:1124	The main chain of ABPS-21 was made up of → 4)-β-D-Galp-(1 → 4)-β-D-Glcp-(1 → 4)-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 2)-α-L-Rhap-(1 →, and two side chains α-l-Rhap-(1 → 4)-β-D-Glcp-(1 → and α-D-galpA-(1 → were attached to the backbone chain at O-6 position of 1,4,6-linked β-D-Glcp and 1,4,6-linked β-D-Galp, respectively.
25965461	1	7	theme	Sephacryl	279:287	arg1	chromatography					313:326	Cellulose DEAE-52 and Sephacryl S-300 HR gel-filtration chromatography	257:326	chromatography	313:326	A water-soluble polysaccharide, designated as ABPS-21, was isolated from the stem barks of Acanthopanax brachypus by hot-water extraction and purified by Cellulose DEAE-52 and Sephacryl S-300 HR gel-filtration chromatography, respectively.
25965461	1	8	attach	isolated	162:169	arg2	polysaccharide					119:132	A water-soluble polysaccharide	103:132	A water-soluble polysaccharide	103:132	A water-soluble polysaccharide, designated as ABPS-21, was isolated from the stem barks of Acanthopanax brachypus by hot-water extraction and purified by Cellulose DEAE-52 and Sephacryl S-300 HR gel-filtration chromatography, respectively.
25965461	1	8	attach	isolated	162:169	arg1	barks					185:189	the stem barks	176:189	the stem barks of Acanthopanax brachypus	176:215	A water-soluble polysaccharide, designated as ABPS-21, was isolated from the stem barks of Acanthopanax brachypus by hot-water extraction and purified by Cellulose DEAE-52 and Sephacryl S-300 HR gel-filtration chromatography, respectively.
25965461	5	9	theme	O-6	1062:1064	arg1	position					1066:1073	O-6 position	1062:1073	O-6 position of 1,4,6-linked β-D-Glcp and 1,4,6-linked β-D-Galp	1062:1124	The main chain of ABPS-21 was made up of → 4)-β-D-Galp-(1 → 4)-β-D-Glcp-(1 → 4)-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 2)-α-L-Rhap-(1 →, and two side chains α-l-Rhap-(1 → 4)-β-D-Glcp-(1 → and α-D-galpA-(1 → were attached to the backbone chain at O-6 position of 1,4,6-linked β-D-Glcp and 1,4,6-linked β-D-Galp, respectively.
25965461	1	10	theme	S-300	289:293	arg1	chromatography					313:326	Cellulose DEAE-52 and Sephacryl S-300 HR gel-filtration chromatography	257:326	chromatography	313:326	A water-soluble polysaccharide, designated as ABPS-21, was isolated from the stem barks of Acanthopanax brachypus by hot-water extraction and purified by Cellulose DEAE-52 and Sephacryl S-300 HR gel-filtration chromatography, respectively.
25965461	4	11	theme	galacturonic	703:714	arg1	acid					716:719	galacturonic acid	703:719	galacturonic acid	703:719	The result showed that ABPS-21 was a homogeneous heteropolysaccharide including galactose, glucose, rhamnose and galacturonic acid in a molar ratio of 3.0:2.0:2.0:1.0, respectively, with an average molecular weight of 1.06 × 10(5)Da.
25965461	3	12	theme	NMR	572:574	arg1	spectroscopy					576:587	NMR spectroscopy	572:587	NMR spectroscopy	572:587	The structure was elucidated based on monosaccharide composition and methylation analysis, partial acid hydrolysis, periodate oxidation, Smith degradation, IR and NMR spectroscopy.
25965461	1	13	theme	stem	180:183	arg1	barks					185:189	the stem barks	176:189	the stem barks of Acanthopanax brachypus	176:215	A water-soluble polysaccharide, designated as ABPS-21, was isolated from the stem barks of Acanthopanax brachypus by hot-water extraction and purified by Cellulose DEAE-52 and Sephacryl S-300 HR gel-filtration chromatography, respectively.
25965461	4	14	theme	homogeneous	627:637	arg1	heteropolysaccharide					639:658	a homogeneous heteropolysaccharide	625:658	a homogeneous heteropolysaccharide including galactose, glucose, rhamnose and galacturonic acid	625:719	The result showed that ABPS-21 was a homogeneous heteropolysaccharide including galactose, glucose, rhamnose and galacturonic acid in a molar ratio of 3.0:2.0:2.0:1.0, respectively, with an average molecular weight of 1.06 × 10(5)Da.
25965461	4	14	theme	homogeneous	627:637	arg1	ABPS-21					613:619	ABPS-21	613:619	ABPS-21	613:619	The result showed that ABPS-21 was a homogeneous heteropolysaccharide including galactose, glucose, rhamnose and galacturonic acid in a molar ratio of 3.0:2.0:2.0:1.0, respectively, with an average molecular weight of 1.06 × 10(5)Da.
25965461	5	15	theme	→	865:865	arg1	-α-L-Rhap-					937:946	→ 4)-β-D-Galp-(1 → 4)-β-D-Glcp-(1 → 4)-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 2)-α-L-Rhap-	865:946	→ 4)-β-D-Galp-(1 → 4)-β-D-Glcp-(1 → 4)-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 2)-α-L-Rhap-(1 →, and two side chains α-l-Rhap-(1 → 4)-β-D-Glcp-(1 →	865:1002	The main chain of ABPS-21 was made up of → 4)-β-D-Galp-(1 → 4)-β-D-Glcp-(1 → 4)-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 2)-α-L-Rhap-(1 →, and two side chains α-l-Rhap-(1 → 4)-β-D-Glcp-(1 → and α-D-galpA-(1 → were attached to the backbone chain at O-6 position of 1,4,6-linked β-D-Glcp and 1,4,6-linked β-D-Galp, respectively.
25965461	6	16	from	brachypus	1192:1200	arg1	ABPS-21					1141:1147	ABPS-21	1141:1147	ABPS-21	1141:1147	ABPS-21, an acidic branched polysaccharide from A. brachypus, was not described up to now.
25965461	6	16	from	brachypus	1192:1200	arg1	polysaccharide					1169:1182	an acidic branched polysaccharide	1150:1182	an acidic branched polysaccharide from A. brachypus	1150:1200	ABPS-21, an acidic branched polysaccharide from A. brachypus, was not described up to now.
25965461	5	17	dep	chains	966:971	arg1	α-l-Rhap-					973:981	α-l-Rhap-(1 → 4	973:987	two side chains α-l-Rhap-(1 → 4	957:987	The main chain of ABPS-21 was made up of → 4)-β-D-Galp-(1 → 4)-β-D-Glcp-(1 → 4)-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 2)-α-L-Rhap-(1 →, and two side chains α-l-Rhap-(1 → 4)-β-D-Glcp-(1 → and α-D-galpA-(1 → were attached to the backbone chain at O-6 position of 1,4,6-linked β-D-Glcp and 1,4,6-linked β-D-Galp, respectively.
25965461	5	18	theme	α-D-galpA-	1008:1017	arg1	→					1021:1021	α-D-galpA-(1 →	1008:1021	α-D-galpA-(1 →	1008:1021	The main chain of ABPS-21 was made up of → 4)-β-D-Galp-(1 → 4)-β-D-Glcp-(1 → 4)-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 2)-α-L-Rhap-(1 →, and two side chains α-l-Rhap-(1 → 4)-β-D-Glcp-(1 → and α-D-galpA-(1 → were attached to the backbone chain at O-6 position of 1,4,6-linked β-D-Glcp and 1,4,6-linked β-D-Galp, respectively.
25965461	4	19	theme	molecular	788:796	arg1	weight					798:803	an average molecular weight	777:803	an average molecular weight of 1.06 × 10(5)Da	777:821	The result showed that ABPS-21 was a homogeneous heteropolysaccharide including galactose, glucose, rhamnose and galacturonic acid in a molar ratio of 3.0:2.0:2.0:1.0, respectively, with an average molecular weight of 1.06 × 10(5)Da.
25965461	4	20	theme	molar	726:730	arg1	ratio					732:736	a molar ratio	724:736	a molar ratio of 3.0:2.0:2.0:1.0, respectively	724:769	The result showed that ABPS-21 was a homogeneous heteropolysaccharide including galactose, glucose, rhamnose and galacturonic acid in a molar ratio of 3.0:2.0:2.0:1.0, respectively, with an average molecular weight of 1.06 × 10(5)Da.
25965461	5	21	theme	main	828:831	arg1	chain					833:837	The main chain	824:837	The main chain of ABPS-21	824:848	The main chain of ABPS-21 was made up of → 4)-β-D-Galp-(1 → 4)-β-D-Glcp-(1 → 4)-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 2)-α-L-Rhap-(1 →, and two side chains α-l-Rhap-(1 → 4)-β-D-Glcp-(1 → and α-D-galpA-(1 → were attached to the backbone chain at O-6 position of 1,4,6-linked β-D-Glcp and 1,4,6-linked β-D-Galp, respectively.
25965461	1	22	theme	HR	295:296	arg1	chromatography					313:326	Cellulose DEAE-52 and Sephacryl S-300 HR gel-filtration chromatography	257:326	chromatography	313:326	A water-soluble polysaccharide, designated as ABPS-21, was isolated from the stem barks of Acanthopanax brachypus by hot-water extraction and purified by Cellulose DEAE-52 and Sephacryl S-300 HR gel-filtration chromatography, respectively.
25965461	1	23	theme	water-soluble	105:117	arg1	polysaccharide					119:132	A water-soluble polysaccharide	103:132	A water-soluble polysaccharide	103:132	A water-soluble polysaccharide, designated as ABPS-21, was isolated from the stem barks of Acanthopanax brachypus by hot-water extraction and purified by Cellulose DEAE-52 and Sephacryl S-300 HR gel-filtration chromatography, respectively.
25965461	3	24	theme	methylation	478:488	arg1	analysis					490:497	methylation analysis	478:497	methylation analysis	478:497	The structure was elucidated based on monosaccharide composition and methylation analysis, partial acid hydrolysis, periodate oxidation, Smith degradation, IR and NMR spectroscopy.
25965461	1	25	theme	gel-filtration	298:311	arg1	chromatography					313:326	Cellulose DEAE-52 and Sephacryl S-300 HR gel-filtration chromatography	257:326	chromatography	313:326	A water-soluble polysaccharide, designated as ABPS-21, was isolated from the stem barks of Acanthopanax brachypus by hot-water extraction and purified by Cellulose DEAE-52 and Sephacryl S-300 HR gel-filtration chromatography, respectively.
25965461	6	26	dep	now	1227:1229	arg1	up					1221:1222	up	1221:1222	up	1221:1222	ABPS-21, an acidic branched polysaccharide from A. brachypus, was not described up to now.
25965461	5	27	dep	-β-D-Galp-	869:878	arg1	4					867:867	4	867:867	4	867:867	The main chain of ABPS-21 was made up of → 4)-β-D-Galp-(1 → 4)-β-D-Glcp-(1 → 4)-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 2)-α-L-Rhap-(1 →, and two side chains α-l-Rhap-(1 → 4)-β-D-Glcp-(1 → and α-D-galpA-(1 → were attached to the backbone chain at O-6 position of 1,4,6-linked β-D-Glcp and 1,4,6-linked β-D-Galp, respectively.
25965461	1	28	theme	brachypus	207:215	arg1	barks					185:189	the stem barks	176:189	the stem barks of Acanthopanax brachypus	176:215	A water-soluble polysaccharide, designated as ABPS-21, was isolated from the stem barks of Acanthopanax brachypus by hot-water extraction and purified by Cellulose DEAE-52 and Sephacryl S-300 HR gel-filtration chromatography, respectively.
25965461	4	29	theme	average	780:786	arg1	weight					798:803	an average molecular weight	777:803	an average molecular weight of 1.06 × 10(5)Da	777:821	The result showed that ABPS-21 was a homogeneous heteropolysaccharide including galactose, glucose, rhamnose and galacturonic acid in a molar ratio of 3.0:2.0:2.0:1.0, respectively, with an average molecular weight of 1.06 × 10(5)Da.
25965461	5	30	theme	→	899:899	arg1	-β-D-Glcp-					886:895	-β-D-Glcp-	886:895	1 → 4)-β-D-Glcp-(1 → 4)-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 2	880:935	The main chain of ABPS-21 was made up of → 4)-β-D-Galp-(1 → 4)-β-D-Glcp-(1 → 4)-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 2)-α-L-Rhap-(1 →, and two side chains α-l-Rhap-(1 → 4)-β-D-Glcp-(1 → and α-D-galpA-(1 → were attached to the backbone chain at O-6 position of 1,4,6-linked β-D-Glcp and 1,4,6-linked β-D-Galp, respectively.
25965461	5	30	theme	→	899:899	arg1	-β-D-Galp-					903:912	1 → 4)-β-D-Galp-(1 → 4	897:918	1 → 4)-β-D-Galp-(1 → 4	897:918	The main chain of ABPS-21 was made up of → 4)-β-D-Galp-(1 → 4)-β-D-Glcp-(1 → 4)-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 2)-α-L-Rhap-(1 →, and two side chains α-l-Rhap-(1 → 4)-β-D-Glcp-(1 → and α-D-galpA-(1 → were attached to the backbone chain at O-6 position of 1,4,6-linked β-D-Glcp and 1,4,6-linked β-D-Galp, respectively.
25965461	5	31	link	1,4,6-linked	1078:1089	arg1	β-D-Glcp					1091:1098	1,4,6-linked β-D-Glcp	1078:1098	1,4,6-linked β-D-Glcp	1078:1098	The main chain of ABPS-21 was made up of → 4)-β-D-Galp-(1 → 4)-β-D-Glcp-(1 → 4)-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 2)-α-L-Rhap-(1 →, and two side chains α-l-Rhap-(1 → 4)-β-D-Glcp-(1 → and α-D-galpA-(1 → were attached to the backbone chain at O-6 position of 1,4,6-linked β-D-Glcp and 1,4,6-linked β-D-Galp, respectively.
25965461	3	32	theme	monosaccharide	447:460	arg1	composition					462:472	monosaccharide composition	447:472	monosaccharide composition	447:472	The structure was elucidated based on monosaccharide composition and methylation analysis, partial acid hydrolysis, periodate oxidation, Smith degradation, IR and NMR spectroscopy.
25965461	6	33	theme	acidic	1153:1158	arg1	ABPS-21					1141:1147	ABPS-21	1141:1147	ABPS-21	1141:1147	ABPS-21, an acidic branched polysaccharide from A. brachypus, was not described up to now.
25965461	6	33	theme	acidic	1153:1158	arg1	polysaccharide					1169:1182	an acidic branched polysaccharide	1150:1182	an acidic branched polysaccharide from A. brachypus	1150:1200	ABPS-21, an acidic branched polysaccharide from A. brachypus, was not described up to now.
25965461	0	34	from	brachypus	92:100	arg1	purification					11:22	purification	11:22	purification	11:22	Isolation, purification and structural characterization of polysaccharide from Acanthopanax brachypus.
25965461	0	34	from	brachypus	92:100	arg1	characterization					39:54	structural characterization	28:54	structural characterization	28:54	Isolation, purification and structural characterization of polysaccharide from Acanthopanax brachypus.
25965461	0	34	from	brachypus	92:100	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation, purification and structural characterization of polysaccharide from Acanthopanax brachypus.
25965461	1	35	theme	Acanthopanax	194:205	arg1	brachypus					207:215	Acanthopanax brachypus	194:215	Acanthopanax brachypus	194:215	A water-soluble polysaccharide, designated as ABPS-21, was isolated from the stem barks of Acanthopanax brachypus by hot-water extraction and purified by Cellulose DEAE-52 and Sephacryl S-300 HR gel-filtration chromatography, respectively.
25965461	1	36	theme	hot-water	220:228	arg1	extraction					230:239	hot-water extraction	220:239	hot-water extraction	220:239	A water-soluble polysaccharide, designated as ABPS-21, was isolated from the stem barks of Acanthopanax brachypus by hot-water extraction and purified by Cellulose DEAE-52 and Sephacryl S-300 HR gel-filtration chromatography, respectively.
25965461	0	37	theme	structural	28:37	arg1	characterization					39:54	structural characterization	28:54	structural characterization	28:54	Isolation, purification and structural characterization of polysaccharide from Acanthopanax brachypus.
25965461	3	38	theme	acid	508:511	arg1	hydrolysis					513:522	partial acid hydrolysis	500:522	partial acid hydrolysis	500:522	The structure was elucidated based on monosaccharide composition and methylation analysis, partial acid hydrolysis, periodate oxidation, Smith degradation, IR and NMR spectroscopy.
25965461	0	39	theme	polysaccharide	59:72	arg1	purification					11:22	purification	11:22	purification	11:22	Isolation, purification and structural characterization of polysaccharide from Acanthopanax brachypus.
25965461	0	39	theme	polysaccharide	59:72	arg1	characterization					39:54	structural characterization	28:54	structural characterization	28:54	Isolation, purification and structural characterization of polysaccharide from Acanthopanax brachypus.
25965461	0	39	theme	polysaccharide	59:72	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation, purification and structural characterization of polysaccharide from Acanthopanax brachypus.
25965461	5	40	theme	1	1000:1000	arg1	→					1002:1002	→	1002:1002	→	1002:1002	The main chain of ABPS-21 was made up of → 4)-β-D-Galp-(1 → 4)-β-D-Glcp-(1 → 4)-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 2)-α-L-Rhap-(1 →, and two side chains α-l-Rhap-(1 → 4)-β-D-Glcp-(1 → and α-D-galpA-(1 → were attached to the backbone chain at O-6 position of 1,4,6-linked β-D-Glcp and 1,4,6-linked β-D-Galp, respectively.
25965461	5	41	theme	side	961:964	arg1	chains					966:971	two side chains	957:971	two side chains α-l-Rhap-(1 → 4	957:987	The main chain of ABPS-21 was made up of → 4)-β-D-Galp-(1 → 4)-β-D-Glcp-(1 → 4)-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 2)-α-L-Rhap-(1 →, and two side chains α-l-Rhap-(1 → 4)-β-D-Glcp-(1 → and α-D-galpA-(1 → were attached to the backbone chain at O-6 position of 1,4,6-linked β-D-Glcp and 1,4,6-linked β-D-Galp, respectively.
25965461	5	42	dep	→	882:882	arg1	-β-D-Galp-					920:929	-β-D-Galp-(1 → 2	920:935	1 → 4)-β-D-Glcp-(1 → 4)-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 2	880:935	The main chain of ABPS-21 was made up of → 4)-β-D-Galp-(1 → 4)-β-D-Glcp-(1 → 4)-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 2)-α-L-Rhap-(1 →, and two side chains α-l-Rhap-(1 → 4)-β-D-Glcp-(1 → and α-D-galpA-(1 → were attached to the backbone chain at O-6 position of 1,4,6-linked β-D-Glcp and 1,4,6-linked β-D-Galp, respectively.
25965461	5	42	dep	→	882:882	arg1	-β-D-Glcp-					886:895	-β-D-Glcp-	886:895	1 → 4)-β-D-Glcp-(1 → 4)-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 2	880:935	The main chain of ABPS-21 was made up of → 4)-β-D-Galp-(1 → 4)-β-D-Glcp-(1 → 4)-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 2)-α-L-Rhap-(1 →, and two side chains α-l-Rhap-(1 → 4)-β-D-Glcp-(1 → and α-D-galpA-(1 → were attached to the backbone chain at O-6 position of 1,4,6-linked β-D-Glcp and 1,4,6-linked β-D-Galp, respectively.
25965461	5	42	dep	→	882:882	arg1	-β-D-Galp-					903:912	1 → 4)-β-D-Galp-(1 → 4	897:918	1 → 4)-β-D-Galp-(1 → 4	897:918	The main chain of ABPS-21 was made up of → 4)-β-D-Galp-(1 → 4)-β-D-Glcp-(1 → 4)-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 2)-α-L-Rhap-(1 →, and two side chains α-l-Rhap-(1 → 4)-β-D-Glcp-(1 → and α-D-galpA-(1 → were attached to the backbone chain at O-6 position of 1,4,6-linked β-D-Glcp and 1,4,6-linked β-D-Galp, respectively.
25965461	5	42	dep	→	882:882	arg1	4					884:884	4	884:884	4	884:884	The main chain of ABPS-21 was made up of → 4)-β-D-Galp-(1 → 4)-β-D-Glcp-(1 → 4)-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 2)-α-L-Rhap-(1 →, and two side chains α-l-Rhap-(1 → 4)-β-D-Glcp-(1 → and α-D-galpA-(1 → were attached to the backbone chain at O-6 position of 1,4,6-linked β-D-Glcp and 1,4,6-linked β-D-Galp, respectively.
25965461	5	43	theme	→	1002:1002	arg1	-α-L-Rhap-					937:946	→ 4)-β-D-Galp-(1 → 4)-β-D-Glcp-(1 → 4)-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 2)-α-L-Rhap-	865:946	→ 4)-β-D-Galp-(1 → 4)-β-D-Glcp-(1 → 4)-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 2)-α-L-Rhap-(1 →, and two side chains α-l-Rhap-(1 → 4)-β-D-Glcp-(1 →	865:1002	The main chain of ABPS-21 was made up of → 4)-β-D-Galp-(1 → 4)-β-D-Glcp-(1 → 4)-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 2)-α-L-Rhap-(1 →, and two side chains α-l-Rhap-(1 → 4)-β-D-Glcp-(1 → and α-D-galpA-(1 → were attached to the backbone chain at O-6 position of 1,4,6-linked β-D-Glcp and 1,4,6-linked β-D-Galp, respectively.
25965461	3	44	theme	Smith	546:550	arg1	degradation					552:562	Smith degradation	546:562	Smith degradation	546:562	The structure was elucidated based on monosaccharide composition and methylation analysis, partial acid hydrolysis, periodate oxidation, Smith degradation, IR and NMR spectroscopy.
25965461	5	45	attach	attached	1028:1035	arg2	→					1021:1021	α-D-galpA-(1 →	1008:1021	α-D-galpA-(1 →	1008:1021	The main chain of ABPS-21 was made up of → 4)-β-D-Galp-(1 → 4)-β-D-Glcp-(1 → 4)-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 2)-α-L-Rhap-(1 →, and two side chains α-l-Rhap-(1 → 4)-β-D-Glcp-(1 → and α-D-galpA-(1 → were attached to the backbone chain at O-6 position of 1,4,6-linked β-D-Glcp and 1,4,6-linked β-D-Galp, respectively.
25965461	5	45	attach	attached	1028:1035	arg1	chain					1053:1057	the backbone chain	1040:1057	the backbone chain	1040:1057	The main chain of ABPS-21 was made up of → 4)-β-D-Galp-(1 → 4)-β-D-Glcp-(1 → 4)-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 2)-α-L-Rhap-(1 →, and two side chains α-l-Rhap-(1 → 4)-β-D-Glcp-(1 → and α-D-galpA-(1 → were attached to the backbone chain at O-6 position of 1,4,6-linked β-D-Glcp and 1,4,6-linked β-D-Galp, respectively.
25965461	5	45	attach	attached	1028:1035	arg3	position					1066:1073	O-6 position	1062:1073	O-6 position of 1,4,6-linked β-D-Glcp and 1,4,6-linked β-D-Galp	1062:1124	The main chain of ABPS-21 was made up of → 4)-β-D-Galp-(1 → 4)-β-D-Glcp-(1 → 4)-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 2)-α-L-Rhap-(1 →, and two side chains α-l-Rhap-(1 → 4)-β-D-Glcp-(1 → and α-D-galpA-(1 → were attached to the backbone chain at O-6 position of 1,4,6-linked β-D-Glcp and 1,4,6-linked β-D-Galp, respectively.
25965461	4	46	with	heteropolysaccharide	639:658	arg1	weight					798:803	an average molecular weight	777:803	an average molecular weight of 1.06 × 10(5)Da	777:821	The result showed that ABPS-21 was a homogeneous heteropolysaccharide including galactose, glucose, rhamnose and galacturonic acid in a molar ratio of 3.0:2.0:2.0:1.0, respectively, with an average molecular weight of 1.06 × 10(5)Da.
25965461	2	47	theme	molecular	363:371	arg1	weight					373:378	molecular weight	363:378	molecular weight	363:378	The homogeneity and molecular weight were determined using HPGPC.
25965461	5	48	theme	1,4,6-linked	1104:1115	arg1	β-D-Galp					1117:1124	1,4,6-linked β-D-Galp	1104:1124	1,4,6-linked β-D-Galp	1104:1124	The main chain of ABPS-21 was made up of → 4)-β-D-Galp-(1 → 4)-β-D-Glcp-(1 → 4)-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 2)-α-L-Rhap-(1 →, and two side chains α-l-Rhap-(1 → 4)-β-D-Glcp-(1 → and α-D-galpA-(1 → were attached to the backbone chain at O-6 position of 1,4,6-linked β-D-Glcp and 1,4,6-linked β-D-Galp, respectively.
25965461	5	49	theme	backbone	1044:1051	arg1	chain					1053:1057	the backbone chain	1040:1057	the backbone chain	1040:1057	The main chain of ABPS-21 was made up of → 4)-β-D-Galp-(1 → 4)-β-D-Glcp-(1 → 4)-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 2)-α-L-Rhap-(1 →, and two side chains α-l-Rhap-(1 → 4)-β-D-Glcp-(1 → and α-D-galpA-(1 → were attached to the backbone chain at O-6 position of 1,4,6-linked β-D-Glcp and 1,4,6-linked β-D-Galp, respectively.
25965461	5	50	theme	β-D-Galp	1117:1124	arg1	position					1066:1073	O-6 position	1062:1073	O-6 position of 1,4,6-linked β-D-Glcp and 1,4,6-linked β-D-Galp	1062:1124	The main chain of ABPS-21 was made up of → 4)-β-D-Galp-(1 → 4)-β-D-Glcp-(1 → 4)-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 2)-α-L-Rhap-(1 →, and two side chains α-l-Rhap-(1 → 4)-β-D-Glcp-(1 → and α-D-galpA-(1 → were attached to the backbone chain at O-6 position of 1,4,6-linked β-D-Glcp and 1,4,6-linked β-D-Galp, respectively.
25965461	3	51	theme	periodate	525:533	arg1	oxidation					535:543	periodate oxidation	525:543	periodate oxidation	525:543	The structure was elucidated based on monosaccharide composition and methylation analysis, partial acid hydrolysis, periodate oxidation, Smith degradation, IR and NMR spectroscopy.
25965461	0	52	theme	Acanthopanax	79:90	arg1	brachypus					92:100	Acanthopanax brachypus	79:100	Acanthopanax brachypus	79:100	Isolation, purification and structural characterization of polysaccharide from Acanthopanax brachypus.
25965461	5	53	link	1,4,6-linked	1104:1115	arg1	β-D-Galp					1117:1124	1,4,6-linked β-D-Galp	1104:1124	1,4,6-linked β-D-Galp	1104:1124	The main chain of ABPS-21 was made up of → 4)-β-D-Galp-(1 → 4)-β-D-Glcp-(1 → 4)-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 2)-α-L-Rhap-(1 →, and two side chains α-l-Rhap-(1 → 4)-β-D-Glcp-(1 → and α-D-galpA-(1 → were attached to the backbone chain at O-6 position of 1,4,6-linked β-D-Glcp and 1,4,6-linked β-D-Galp, respectively.
25965461	3	54	theme	partial	500:506	arg1	hydrolysis					513:522	partial acid hydrolysis	500:522	partial acid hydrolysis	500:522	The structure was elucidated based on monosaccharide composition and methylation analysis, partial acid hydrolysis, periodate oxidation, Smith degradation, IR and NMR spectroscopy.
25965461	5	55	theme	ABPS-21	842:848	arg1	chain					833:837	The main chain	824:837	The main chain of ABPS-21	824:848	The main chain of ABPS-21 was made up of → 4)-β-D-Galp-(1 → 4)-β-D-Glcp-(1 → 4)-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 2)-α-L-Rhap-(1 →, and two side chains α-l-Rhap-(1 → 4)-β-D-Glcp-(1 → and α-D-galpA-(1 → were attached to the backbone chain at O-6 position of 1,4,6-linked β-D-Glcp and 1,4,6-linked β-D-Galp, respectively.
25965461	5	56	theme	-β-D-Galp-	869:878	arg1	-α-L-Rhap-					937:946	→ 4)-β-D-Galp-(1 → 4)-β-D-Glcp-(1 → 4)-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 2)-α-L-Rhap-	865:946	→ 4)-β-D-Galp-(1 → 4)-β-D-Glcp-(1 → 4)-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 2)-α-L-Rhap-(1 →, and two side chains α-l-Rhap-(1 → 4)-β-D-Glcp-(1 →	865:1002	The main chain of ABPS-21 was made up of → 4)-β-D-Galp-(1 → 4)-β-D-Glcp-(1 → 4)-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 2)-α-L-Rhap-(1 →, and two side chains α-l-Rhap-(1 → 4)-β-D-Glcp-(1 → and α-D-galpA-(1 → were attached to the backbone chain at O-6 position of 1,4,6-linked β-D-Glcp and 1,4,6-linked β-D-Galp, respectively.
25965461	5	57	dep	-α-L-Rhap-	937:946	arg1	chains					966:971	two side chains	957:971	two side chains α-l-Rhap-(1 → 4	957:987	The main chain of ABPS-21 was made up of → 4)-β-D-Galp-(1 → 4)-β-D-Glcp-(1 → 4)-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 2)-α-L-Rhap-(1 →, and two side chains α-l-Rhap-(1 → 4)-β-D-Glcp-(1 → and α-D-galpA-(1 → were attached to the backbone chain at O-6 position of 1,4,6-linked β-D-Glcp and 1,4,6-linked β-D-Galp, respectively.
25965461	5	57	dep	-α-L-Rhap-	937:946	arg1	→					950:950	1 →	948:950	1 →	948:950	The main chain of ABPS-21 was made up of → 4)-β-D-Galp-(1 → 4)-β-D-Glcp-(1 → 4)-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 2)-α-L-Rhap-(1 →, and two side chains α-l-Rhap-(1 → 4)-β-D-Glcp-(1 → and α-D-galpA-(1 → were attached to the backbone chain at O-6 position of 1,4,6-linked β-D-Glcp and 1,4,6-linked β-D-Galp, respectively.
25965461	4	58	theme	Da	820:821	arg1	weight					798:803	an average molecular weight	777:803	an average molecular weight of 1.06 × 10(5)Da	777:821	The result showed that ABPS-21 was a homogeneous heteropolysaccharide including galactose, glucose, rhamnose and galacturonic acid in a molar ratio of 3.0:2.0:2.0:1.0, respectively, with an average molecular weight of 1.06 × 10(5)Da.
25965461	4	59	dep	10	815:816	arg1	5					818:818	5	818:818	5	818:818	The result showed that ABPS-21 was a homogeneous heteropolysaccharide including galactose, glucose, rhamnose and galacturonic acid in a molar ratio of 3.0:2.0:2.0:1.0, respectively, with an average molecular weight of 1.06 × 10(5)Da.
25965461	1	60	theme	Cellulose	257:265	arg1	DEAE-52					267:273	Cellulose DEAE-52 and Sephacryl S-300 HR gel-filtration chromatography	257:326	DEAE-52	267:273	A water-soluble polysaccharide, designated as ABPS-21, was isolated from the stem barks of Acanthopanax brachypus by hot-water extraction and purified by Cellulose DEAE-52 and Sephacryl S-300 HR gel-filtration chromatography, respectively.
26314909	2	0	theme	-EC-based	413:421	arg1	bio-composites					423:436	the newly developed P(3HB)-EC-based bio-composites	387:436	the newly developed P(3HB)-EC-based bio-composites	387:436	Furthermore, natural phenols e.g., p-4-hydroxybenzoic acid (HBA) and ferulic acid (FA) were grafted onto the newly developed P(3HB)-EC-based bio-composites under laccase-assisted environment without the use of additional initiators or crosslinking agents.
26314909	6	1	theme	soil	1302:1305	arg1	analyses					1314:1321	the soil burial analyses	1298:1321	the soil burial analyses	1298:1321	As for the degradation, an increase in the degradation rate was recorded during the soil burial analyses over a period of 42 days.
26314909	4	2	theme	3HB	836:838	arg1	-EC					840:842	15FA-g-P(3HB)-EC	827:842	15FA-g-P(3HB)-EC	827:842	In particular, the results of the antibacterial evaluation for the newly developed bio-composites indicated that 20HBA-g-P(3HB)-EC and 15FA-g-P(3HB)-EC bio-composites exerted strong bactericidal and bacteriostatic activity against Gram(-)E. coli NTCT 10418 as compared to the Gram(+)B. subtilis NCTC 3610.
26314909	4	3	dep	E.	930:931	arg1	coli					933:936	coli	933:936	coli	933:936	In particular, the results of the antibacterial evaluation for the newly developed bio-composites indicated that 20HBA-g-P(3HB)-EC and 15FA-g-P(3HB)-EC bio-composites exerted strong bactericidal and bacteriostatic activity against Gram(-)E. coli NTCT 10418 as compared to the Gram(+)B. subtilis NCTC 3610.
26314909	4	4	theme	B.	975:976	arg1	Gram					968:971	the Gram	964:971	the Gram	964:971	In particular, the results of the antibacterial evaluation for the newly developed bio-composites indicated that 20HBA-g-P(3HB)-EC and 15FA-g-P(3HB)-EC bio-composites exerted strong bactericidal and bacteriostatic activity against Gram(-)E. coli NTCT 10418 as compared to the Gram(+)B. subtilis NCTC 3610.
26314909	5	5	theme	skin	1159:1162	arg1	cells					1164:1168	human keratinocyte-like HaCaT skin cells	1129:1168	human keratinocyte-like HaCaT skin cells	1129:1168	This study shows further that at various phenolic concentrations the newly synthesised bio-composites remained cytocompatible with human keratinocyte-like HaCaT skin cells, as 100% cell viability was recorded, in vitro.
26314909	4	6	theme	NTCT	938:941	arg1	10418					943:947	E. coli NTCT 10418	930:947	E. coli NTCT 10418	930:947	In particular, the results of the antibacterial evaluation for the newly developed bio-composites indicated that 20HBA-g-P(3HB)-EC and 15FA-g-P(3HB)-EC bio-composites exerted strong bactericidal and bacteriostatic activity against Gram(-)E. coli NTCT 10418 as compared to the Gram(+)B. subtilis NCTC 3610.
26314909	7	7	theme	reported	1381:1388	arg1	bio-composites					1390:1403	the reported bio-composites	1377:1403	the reported bio-composites	1377:1403	These findings suggest that the reported bio-composites have great potential for use in wound healing; covering the affected skin area which may favour tissue repair over shorter periods.
26314909	1	8	theme	poly3-hydroxybutyrate	171:191	arg1	3HB					196:198	3HB	196:198	3HB	196:198	A series of bio-composites including poly3-hydroxybutyrate [P(3HB)] grafted ethyl cellulose (EC) stated as P(3HB)-EC were successfully synthesised.
26314909	1	8	theme	poly3-hydroxybutyrate	171:191	arg1	[P					193:194	poly3-hydroxybutyrate [P	171:194	poly3-hydroxybutyrate [P(3HB)] grafted ethyl cellulose (EC) stated as P(3HB)-EC	171:249	A series of bio-composites including poly3-hydroxybutyrate [P(3HB)] grafted ethyl cellulose (EC) stated as P(3HB)-EC were successfully synthesised.
26314909	1	9	theme	P	241:241	arg1	-EC					247:249	P(3HB)-EC	241:249	P(3HB)-EC	241:249	A series of bio-composites including poly3-hydroxybutyrate [P(3HB)] grafted ethyl cellulose (EC) stated as P(3HB)-EC were successfully synthesised.
26314909	0	10	theme	free	102:105	arg1	healing					113:119	infection free wound healing	92:119	infection free wound healing application	92:131	Poly(3-hydroxybutyrate)-ethyl cellulose based bio-composites with novel characteristics for infection free wound healing application.
26314909	6	11	from	increase	1245:1252	arg1	rate					1273:1276	the degradation rate	1257:1276	the degradation rate	1257:1276	As for the degradation, an increase in the degradation rate was recorded during the soil burial analyses over a period of 42 days.
26314909	4	12	theme	-EC	840:842	arg1	bio-composites					844:857	20HBA-g-P(3HB)-EC and 15FA-g-P(3HB)-EC bio-composites	805:857	20HBA-g-P(3HB)-EC and 15FA-g-P(3HB)-EC bio-composites	805:857	In particular, the results of the antibacterial evaluation for the newly developed bio-composites indicated that 20HBA-g-P(3HB)-EC and 15FA-g-P(3HB)-EC bio-composites exerted strong bactericidal and bacteriostatic activity against Gram(-)E. coli NTCT 10418 as compared to the Gram(+)B. subtilis NCTC 3610.
26314909	5	13	with	cytocompatible	1109:1122	arg1	cells					1164:1168	human keratinocyte-like HaCaT skin cells	1129:1168	human keratinocyte-like HaCaT skin cells	1129:1168	This study shows further that at various phenolic concentrations the newly synthesised bio-composites remained cytocompatible with human keratinocyte-like HaCaT skin cells, as 100% cell viability was recorded, in vitro.
26314909	2	14	dep	acid	336:339	arg1	e.g.					311:314	e.g.	311:314	e.g.	311:314	Furthermore, natural phenols e.g., p-4-hydroxybenzoic acid (HBA) and ferulic acid (FA) were grafted onto the newly developed P(3HB)-EC-based bio-composites under laccase-assisted environment without the use of additional initiators or crosslinking agents.
26314909	4	15	theme	NCTC	987:990	arg1	3610					992:995	the Gram(+)B. subtilis NCTC 3610	964:995	the Gram(+)B. subtilis NCTC 3610	964:995	In particular, the results of the antibacterial evaluation for the newly developed bio-composites indicated that 20HBA-g-P(3HB)-EC and 15FA-g-P(3HB)-EC bio-composites exerted strong bactericidal and bacteriostatic activity against Gram(-)E. coli NTCT 10418 as compared to the Gram(+)B. subtilis NCTC 3610.
26314909	0	16	theme	infection	92:100	arg1	healing					113:119	infection free wound healing	92:119	infection free wound healing application	92:131	Poly(3-hydroxybutyrate)-ethyl cellulose based bio-composites with novel characteristics for infection free wound healing application.
26314909	4	17	theme	antibacterial	726:738	arg1	evaluation					740:749	the antibacterial evaluation	722:749	the antibacterial evaluation for the newly developed bio-composites	722:788	In particular, the results of the antibacterial evaluation for the newly developed bio-composites indicated that 20HBA-g-P(3HB)-EC and 15FA-g-P(3HB)-EC bio-composites exerted strong bactericidal and bacteriostatic activity against Gram(-)E. coli NTCT 10418 as compared to the Gram(+)B. subtilis NCTC 3610.
26314909	1	18	theme	3HB	243:245	arg1	-EC					247:249	P(3HB)-EC	241:249	P(3HB)-EC	241:249	A series of bio-composites including poly3-hydroxybutyrate [P(3HB)] grafted ethyl cellulose (EC) stated as P(3HB)-EC were successfully synthesised.
26314909	0	19	theme	healing	113:119	arg1	application					121:131	infection free wound healing application	92:131	infection free wound healing application	92:131	Poly(3-hydroxybutyrate)-ethyl cellulose based bio-composites with novel characteristics for infection free wound healing application.
26314909	4	20	theme	-EC	819:821	arg1	bio-composites					844:857	20HBA-g-P(3HB)-EC and 15FA-g-P(3HB)-EC bio-composites	805:857	20HBA-g-P(3HB)-EC and 15FA-g-P(3HB)-EC bio-composites	805:857	In particular, the results of the antibacterial evaluation for the newly developed bio-composites indicated that 20HBA-g-P(3HB)-EC and 15FA-g-P(3HB)-EC bio-composites exerted strong bactericidal and bacteriostatic activity against Gram(-)E. coli NTCT 10418 as compared to the Gram(+)B. subtilis NCTC 3610.
26314909	1	21	theme	[P	193:194	arg1	EC					227:228	EC	227:228	EC	227:228	A series of bio-composites including poly3-hydroxybutyrate [P(3HB)] grafted ethyl cellulose (EC) stated as P(3HB)-EC were successfully synthesised.
26314909	1	21	theme	[P	193:194	arg1	cellulose					216:224	poly3-hydroxybutyrate [P(3HB)] grafted ethyl cellulose	171:224	poly3-hydroxybutyrate [P(3HB)] grafted ethyl cellulose (EC) stated as P(3HB)-EC	171:249	A series of bio-composites including poly3-hydroxybutyrate [P(3HB)] grafted ethyl cellulose (EC) stated as P(3HB)-EC were successfully synthesised.
26314909	0	22	theme	wound	107:111	arg1	healing					113:119	infection free wound healing	92:119	infection free wound healing application	92:131	Poly(3-hydroxybutyrate)-ethyl cellulose based bio-composites with novel characteristics for infection free wound healing application.
26314909	7	23	theme	skin	1474:1477	arg1	area					1479:1482	the affected skin area	1461:1482	the affected skin area which may favour tissue repair over shorter periods	1461:1534	These findings suggest that the reported bio-composites have great potential for use in wound healing; covering the affected skin area which may favour tissue repair over shorter periods.
26314909	7	24	theme	tissue	1501:1506	arg1	repair					1508:1513	tissue repair	1501:1513	tissue repair	1501:1513	These findings suggest that the reported bio-composites have great potential for use in wound healing; covering the affected skin area which may favour tissue repair over shorter periods.
26314909	2	25	theme	ferulic	351:357	arg1	FA					365:366	FA	365:366	FA	365:366	Furthermore, natural phenols e.g., p-4-hydroxybenzoic acid (HBA) and ferulic acid (FA) were grafted onto the newly developed P(3HB)-EC-based bio-composites under laccase-assisted environment without the use of additional initiators or crosslinking agents.
26314909	2	25	theme	ferulic	351:357	arg1	acid					359:362	ferulic acid	351:362	ferulic acid (FA)	351:367	Furthermore, natural phenols e.g., p-4-hydroxybenzoic acid (HBA) and ferulic acid (FA) were grafted onto the newly developed P(3HB)-EC-based bio-composites under laccase-assisted environment without the use of additional initiators or crosslinking agents.
26314909	5	26	theme	various	1031:1037	arg1	concentrations					1048:1061	various phenolic concentrations	1031:1061	various phenolic concentrations	1031:1061	This study shows further that at various phenolic concentrations the newly synthesised bio-composites remained cytocompatible with human keratinocyte-like HaCaT skin cells, as 100% cell viability was recorded, in vitro.
26314909	4	27	theme	15FA-g-P	827:834	arg1	-EC					840:842	15FA-g-P(3HB)-EC	827:842	15FA-g-P(3HB)-EC	827:842	In particular, the results of the antibacterial evaluation for the newly developed bio-composites indicated that 20HBA-g-P(3HB)-EC and 15FA-g-P(3HB)-EC bio-composites exerted strong bactericidal and bacteriostatic activity against Gram(-)E. coli NTCT 10418 as compared to the Gram(+)B. subtilis NCTC 3610.
26314909	3	28	from	features	643:650	arg1	soil					686:689	soil	686:689	soil	686:689	The phenol grafted bio-composites were critically evaluated for their antibacterial and biocompatibility features as well as their degradability in soil.
26314909	4	29	theme	Gram	968:971	arg1	3610					992:995	the Gram(+)B. subtilis NCTC 3610	964:995	the Gram(+)B. subtilis NCTC 3610	964:995	In particular, the results of the antibacterial evaluation for the newly developed bio-composites indicated that 20HBA-g-P(3HB)-EC and 15FA-g-P(3HB)-EC bio-composites exerted strong bactericidal and bacteriostatic activity against Gram(-)E. coli NTCT 10418 as compared to the Gram(+)B. subtilis NCTC 3610.
26314909	6	30	theme	degradation	1261:1271	arg1	rate					1273:1276	the degradation rate	1257:1276	the degradation rate	1257:1276	As for the degradation, an increase in the degradation rate was recorded during the soil burial analyses over a period of 42 days.
26314909	4	31	dep	Gram	968:971	arg1	subtilis					978:985	subtilis	978:985	subtilis	978:985	In particular, the results of the antibacterial evaluation for the newly developed bio-composites indicated that 20HBA-g-P(3HB)-EC and 15FA-g-P(3HB)-EC bio-composites exerted strong bactericidal and bacteriostatic activity against Gram(-)E. coli NTCT 10418 as compared to the Gram(+)B. subtilis NCTC 3610.
26314909	7	32	theme	great	1410:1414	arg1	potential					1416:1424	great potential	1410:1424	great potential for use	1410:1432	These findings suggest that the reported bio-composites have great potential for use in wound healing; covering the affected skin area which may favour tissue repair over shorter periods.
26314909	7	33	contain	have	1405:1408	arg1	bio-composites					1390:1403	the reported bio-composites	1377:1403	the reported bio-composites	1377:1403	These findings suggest that the reported bio-composites have great potential for use in wound healing; covering the affected skin area which may favour tissue repair over shorter periods.
26314909	7	33	contain	have	1405:1408	arg2	potential					1416:1424	great potential	1410:1424	great potential for use	1410:1432	These findings suggest that the reported bio-composites have great potential for use in wound healing; covering the affected skin area which may favour tissue repair over shorter periods.
26314909	6	34	theme	days	1343:1346	arg1	period					1330:1335	a period	1328:1335	a period of 42 days	1328:1346	As for the degradation, an increase in the degradation rate was recorded during the soil burial analyses over a period of 42 days.
26314909	5	35	theme	human	1129:1133	arg1	cells					1164:1168	human keratinocyte-like HaCaT skin cells	1129:1168	human keratinocyte-like HaCaT skin cells	1129:1168	This study shows further that at various phenolic concentrations the newly synthesised bio-composites remained cytocompatible with human keratinocyte-like HaCaT skin cells, as 100% cell viability was recorded, in vitro.
26314909	2	36	theme	initiators	503:512	arg1	use					485:487	the use	481:487	the use of additional initiators or crosslinking agents	481:535	Furthermore, natural phenols e.g., p-4-hydroxybenzoic acid (HBA) and ferulic acid (FA) were grafted onto the newly developed P(3HB)-EC-based bio-composites under laccase-assisted environment without the use of additional initiators or crosslinking agents.
26314909	7	37	theme	affected	1465:1472	arg1	area					1479:1482	the affected skin area	1461:1482	the affected skin area which may favour tissue repair over shorter periods	1461:1534	These findings suggest that the reported bio-composites have great potential for use in wound healing; covering the affected skin area which may favour tissue repair over shorter periods.
26314909	4	38	theme	20HBA-g-P	805:813	arg1	-EC					819:821	20HBA-g-P(3HB)-EC	805:821	20HBA-g-P(3HB)-EC	805:821	In particular, the results of the antibacterial evaluation for the newly developed bio-composites indicated that 20HBA-g-P(3HB)-EC and 15FA-g-P(3HB)-EC bio-composites exerted strong bactericidal and bacteriostatic activity against Gram(-)E. coli NTCT 10418 as compared to the Gram(+)B. subtilis NCTC 3610.
26314909	5	39	theme	keratinocyte-like	1135:1151	arg1	cells					1164:1168	human keratinocyte-like HaCaT skin cells	1129:1168	human keratinocyte-like HaCaT skin cells	1129:1168	This study shows further that at various phenolic concentrations the newly synthesised bio-composites remained cytocompatible with human keratinocyte-like HaCaT skin cells, as 100% cell viability was recorded, in vitro.
26314909	2	40	theme	additional	492:501	arg1	initiators					503:512	additional initiators	492:512	additional initiators	492:512	Furthermore, natural phenols e.g., p-4-hydroxybenzoic acid (HBA) and ferulic acid (FA) were grafted onto the newly developed P(3HB)-EC-based bio-composites under laccase-assisted environment without the use of additional initiators or crosslinking agents.
26314909	2	41	theme	p-4-hydroxybenzoic	317:334	arg1	acid					336:339	p-4-hydroxybenzoic acid	317:339	p-4-hydroxybenzoic acid (HBA)	317:345	Furthermore, natural phenols e.g., p-4-hydroxybenzoic acid (HBA) and ferulic acid (FA) were grafted onto the newly developed P(3HB)-EC-based bio-composites under laccase-assisted environment without the use of additional initiators or crosslinking agents.
26314909	2	41	theme	p-4-hydroxybenzoic	317:334	arg1	HBA					342:344	HBA	342:344	HBA	342:344	Furthermore, natural phenols e.g., p-4-hydroxybenzoic acid (HBA) and ferulic acid (FA) were grafted onto the newly developed P(3HB)-EC-based bio-composites under laccase-assisted environment without the use of additional initiators or crosslinking agents.
26314909	1	42	theme	grafted	202:208	arg1	EC					227:228	EC	227:228	EC	227:228	A series of bio-composites including poly3-hydroxybutyrate [P(3HB)] grafted ethyl cellulose (EC) stated as P(3HB)-EC were successfully synthesised.
26314909	1	42	theme	grafted	202:208	arg1	cellulose					216:224	poly3-hydroxybutyrate [P(3HB)] grafted ethyl cellulose	171:224	poly3-hydroxybutyrate [P(3HB)] grafted ethyl cellulose (EC) stated as P(3HB)-EC	171:249	A series of bio-composites including poly3-hydroxybutyrate [P(3HB)] grafted ethyl cellulose (EC) stated as P(3HB)-EC were successfully synthesised.
26314909	0	43	theme	cellulose	30:38	arg1	based					40:44	Poly(3-hydroxybutyrate)-ethyl cellulose based	0:44	Poly(3-hydroxybutyrate)-ethyl cellulose based	0:44	Poly(3-hydroxybutyrate)-ethyl cellulose based bio-composites with novel characteristics for infection free wound healing application.
26314909	5	44	theme	HaCaT	1153:1157	arg1	cells					1164:1168	human keratinocyte-like HaCaT skin cells	1129:1168	human keratinocyte-like HaCaT skin cells	1129:1168	This study shows further that at various phenolic concentrations the newly synthesised bio-composites remained cytocompatible with human keratinocyte-like HaCaT skin cells, as 100% cell viability was recorded, in vitro.
26314909	3	45	theme	biocompatibility	626:641	arg1	features					643:650	their antibacterial and biocompatibility features	602:650	their antibacterial and biocompatibility features as well as their degradability in soil	602:689	The phenol grafted bio-composites were critically evaluated for their antibacterial and biocompatibility features as well as their degradability in soil.
26314909	3	46	theme	grafted	549:555	arg1	bio-composites					557:570	The phenol grafted bio-composites	538:570	The phenol grafted bio-composites	538:570	The phenol grafted bio-composites were critically evaluated for their antibacterial and biocompatibility features as well as their degradability in soil.
26314909	1	47	theme	ethyl	210:214	arg1	EC					227:228	EC	227:228	EC	227:228	A series of bio-composites including poly3-hydroxybutyrate [P(3HB)] grafted ethyl cellulose (EC) stated as P(3HB)-EC were successfully synthesised.
26314909	1	47	theme	ethyl	210:214	arg1	cellulose					216:224	poly3-hydroxybutyrate [P(3HB)] grafted ethyl cellulose	171:224	poly3-hydroxybutyrate [P(3HB)] grafted ethyl cellulose (EC) stated as P(3HB)-EC	171:249	A series of bio-composites including poly3-hydroxybutyrate [P(3HB)] grafted ethyl cellulose (EC) stated as P(3HB)-EC were successfully synthesised.
26314909	0	48	theme	-ethyl	23:28	arg1	based					40:44	Poly(3-hydroxybutyrate)-ethyl cellulose based	0:44	Poly(3-hydroxybutyrate)-ethyl cellulose based	0:44	Poly(3-hydroxybutyrate)-ethyl cellulose based bio-composites with novel characteristics for infection free wound healing application.
26314909	5	49	theme	synthesised	1073:1083	arg1	bio-composites					1085:1098	the newly synthesised bio-composites	1063:1098	the newly synthesised bio-composites	1063:1098	This study shows further that at various phenolic concentrations the newly synthesised bio-composites remained cytocompatible with human keratinocyte-like HaCaT skin cells, as 100% cell viability was recorded, in vitro.
26314909	3	50	from	degradability	669:681	arg1	soil					686:689	soil	686:689	soil	686:689	The phenol grafted bio-composites were critically evaluated for their antibacterial and biocompatibility features as well as their degradability in soil.
26314909	7	51	theme	wound	1437:1441	arg1	healing					1443:1449	wound healing	1437:1449	wound healing	1437:1449	These findings suggest that the reported bio-composites have great potential for use in wound healing; covering the affected skin area which may favour tissue repair over shorter periods.
26314909	2	52	theme	laccase-assisted	444:459	arg1	environment					461:471	laccase-assisted environment	444:471	laccase-assisted environment without the use of additional initiators or crosslinking agents	444:535	Furthermore, natural phenols e.g., p-4-hydroxybenzoic acid (HBA) and ferulic acid (FA) were grafted onto the newly developed P(3HB)-EC-based bio-composites under laccase-assisted environment without the use of additional initiators or crosslinking agents.
26314909	7	53	theme	shorter	1520:1526	arg1	periods					1528:1534	shorter periods	1520:1534	shorter periods	1520:1534	These findings suggest that the reported bio-composites have great potential for use in wound healing; covering the affected skin area which may favour tissue repair over shorter periods.
26314909	3	54	theme	antibacterial	608:620	arg1	features					643:650	their antibacterial and biocompatibility features	602:650	their antibacterial and biocompatibility features as well as their degradability in soil	602:689	The phenol grafted bio-composites were critically evaluated for their antibacterial and biocompatibility features as well as their degradability in soil.
26314909	4	55	theme	3HB	815:817	arg1	-EC					819:821	20HBA-g-P(3HB)-EC	805:821	20HBA-g-P(3HB)-EC	805:821	In particular, the results of the antibacterial evaluation for the newly developed bio-composites indicated that 20HBA-g-P(3HB)-EC and 15FA-g-P(3HB)-EC bio-composites exerted strong bactericidal and bacteriostatic activity against Gram(-)E. coli NTCT 10418 as compared to the Gram(+)B. subtilis NCTC 3610.
26314909	4	56	theme	bactericidal	874:885	arg1	activity					906:913	strong bactericidal and bacteriostatic activity	867:913	strong bactericidal and bacteriostatic activity against Gram(-)E. coli NTCT 10418	867:947	In particular, the results of the antibacterial evaluation for the newly developed bio-composites indicated that 20HBA-g-P(3HB)-EC and 15FA-g-P(3HB)-EC bio-composites exerted strong bactericidal and bacteriostatic activity against Gram(-)E. coli NTCT 10418 as compared to the Gram(+)B. subtilis NCTC 3610.
26314909	2	57	theme	developed	397:405	arg1	bio-composites					423:436	the newly developed P(3HB)-EC-based bio-composites	387:436	the newly developed P(3HB)-EC-based bio-composites	387:436	Furthermore, natural phenols e.g., p-4-hydroxybenzoic acid (HBA) and ferulic acid (FA) were grafted onto the newly developed P(3HB)-EC-based bio-composites under laccase-assisted environment without the use of additional initiators or crosslinking agents.
26314909	2	58	theme	agents	530:535	arg1	use					485:487	the use	481:487	the use of additional initiators or crosslinking agents	481:535	Furthermore, natural phenols e.g., p-4-hydroxybenzoic acid (HBA) and ferulic acid (FA) were grafted onto the newly developed P(3HB)-EC-based bio-composites under laccase-assisted environment without the use of additional initiators or crosslinking agents.
26314909	3	59	theme	phenol	542:547	arg1	bio-composites					557:570	The phenol grafted bio-composites	538:570	The phenol grafted bio-composites	538:570	The phenol grafted bio-composites were critically evaluated for their antibacterial and biocompatibility features as well as their degradability in soil.
26314909	2	60	theme	crosslinking	517:528	arg1	agents					530:535	crosslinking agents	517:535	crosslinking agents	517:535	Furthermore, natural phenols e.g., p-4-hydroxybenzoic acid (HBA) and ferulic acid (FA) were grafted onto the newly developed P(3HB)-EC-based bio-composites under laccase-assisted environment without the use of additional initiators or crosslinking agents.
26314909	4	61	theme	developed	765:773	arg1	bio-composites					775:788	the newly developed bio-composites	755:788	the newly developed bio-composites	755:788	In particular, the results of the antibacterial evaluation for the newly developed bio-composites indicated that 20HBA-g-P(3HB)-EC and 15FA-g-P(3HB)-EC bio-composites exerted strong bactericidal and bacteriostatic activity against Gram(-)E. coli NTCT 10418 as compared to the Gram(+)B. subtilis NCTC 3610.
26314909	5	62	theme	100	1174:1176	arg1	%					1177:1177	%	1177:1177	%	1177:1177	This study shows further that at various phenolic concentrations the newly synthesised bio-composites remained cytocompatible with human keratinocyte-like HaCaT skin cells, as 100% cell viability was recorded, in vitro.
26314909	4	63	theme	strong	867:872	arg1	activity					906:913	strong bactericidal and bacteriostatic activity	867:913	strong bactericidal and bacteriostatic activity against Gram(-)E. coli NTCT 10418	867:947	In particular, the results of the antibacterial evaluation for the newly developed bio-composites indicated that 20HBA-g-P(3HB)-EC and 15FA-g-P(3HB)-EC bio-composites exerted strong bactericidal and bacteriostatic activity against Gram(-)E. coli NTCT 10418 as compared to the Gram(+)B. subtilis NCTC 3610.
26314909	4	64	theme	evaluation	740:749	arg1	results					711:717	the results	707:717	the results of the antibacterial evaluation for the newly developed bio-composites	707:788	In particular, the results of the antibacterial evaluation for the newly developed bio-composites indicated that 20HBA-g-P(3HB)-EC and 15FA-g-P(3HB)-EC bio-composites exerted strong bactericidal and bacteriostatic activity against Gram(-)E. coli NTCT 10418 as compared to the Gram(+)B. subtilis NCTC 3610.
26314909	2	65	theme	natural	295:301	arg1	phenols					303:309	natural phenols	295:309	natural phenols e.g., p-4-hydroxybenzoic acid (HBA) and ferulic acid (FA)	295:367	Furthermore, natural phenols e.g., p-4-hydroxybenzoic acid (HBA) and ferulic acid (FA) were grafted onto the newly developed P(3HB)-EC-based bio-composites under laccase-assisted environment without the use of additional initiators or crosslinking agents.
26314909	5	66	theme	%	1177:1177	arg1	viability					1184:1192	100% cell viability	1174:1192	100% cell viability	1174:1192	This study shows further that at various phenolic concentrations the newly synthesised bio-composites remained cytocompatible with human keratinocyte-like HaCaT skin cells, as 100% cell viability was recorded, in vitro.
26314909	0	67	theme	novel	66:70	arg1	characteristics					72:86	novel characteristics	66:86	novel characteristics for infection free wound healing application	66:131	Poly(3-hydroxybutyrate)-ethyl cellulose based bio-composites with novel characteristics for infection free wound healing application.
26314909	4	68	theme	bacteriostatic	891:904	arg1	activity					906:913	strong bactericidal and bacteriostatic activity	867:913	strong bactericidal and bacteriostatic activity against Gram(-)E. coli NTCT 10418	867:947	In particular, the results of the antibacterial evaluation for the newly developed bio-composites indicated that 20HBA-g-P(3HB)-EC and 15FA-g-P(3HB)-EC bio-composites exerted strong bactericidal and bacteriostatic activity against Gram(-)E. coli NTCT 10418 as compared to the Gram(+)B. subtilis NCTC 3610.
26314909	6	69	theme	burial	1307:1312	arg1	analyses					1314:1321	the soil burial analyses	1298:1321	the soil burial analyses	1298:1321	As for the degradation, an increase in the degradation rate was recorded during the soil burial analyses over a period of 42 days.
26314909	5	70	theme	cell	1179:1182	arg1	viability					1184:1192	100% cell viability	1174:1192	100% cell viability	1174:1192	This study shows further that at various phenolic concentrations the newly synthesised bio-composites remained cytocompatible with human keratinocyte-like HaCaT skin cells, as 100% cell viability was recorded, in vitro.
26314909	4	71	theme	E.	930:931	arg1	10418					943:947	E. coli NTCT 10418	930:947	E. coli NTCT 10418	930:947	In particular, the results of the antibacterial evaluation for the newly developed bio-composites indicated that 20HBA-g-P(3HB)-EC and 15FA-g-P(3HB)-EC bio-composites exerted strong bactericidal and bacteriostatic activity against Gram(-)E. coli NTCT 10418 as compared to the Gram(+)B. subtilis NCTC 3610.
26314909	5	72	theme	phenolic	1039:1046	arg1	concentrations					1048:1061	various phenolic concentrations	1031:1061	various phenolic concentrations	1031:1061	This study shows further that at various phenolic concentrations the newly synthesised bio-composites remained cytocompatible with human keratinocyte-like HaCaT skin cells, as 100% cell viability was recorded, in vitro.
26314909	1	73	theme	bio-composites	146:159	arg1	series					136:141	A series	134:141	A series of bio-composites including poly3-hydroxybutyrate [P(3HB)] grafted ethyl cellulose (EC) stated as P(3HB)-EC	134:249	A series of bio-composites including poly3-hydroxybutyrate [P(3HB)] grafted ethyl cellulose (EC) stated as P(3HB)-EC were successfully synthesised.
26223829	0	0	theme	interactions	94:105	arg1	characterisation					44:59	characterisation	44:59	Structural mechanism of complex assemblies: characterisation of beta-lactoglobulin and pectin interactions.	0:106	Structural mechanism of complex assemblies: characterisation of beta-lactoglobulin and pectin interactions.
26223829	6	1	theme	pectin	1235:1240	arg1	samples					1242:1248	the pectin samples	1231:1248	the pectin samples	1231:1248	Circular dichroism (CD) and intrinsic tryptophan fluorescence were also measured of βLgA during its interaction with the pectin samples, and show that the binding leads to significant conformational changes in βLgA.
26223829	1	2	from	encapsulation	191:203	arg1	systems					243:249	these composite systems	227:249	these composite systems	227:249	Knowledge of how proteins and polysaccharides interact is the key to understanding encapsulation and emulsification in these composite systems and ultimately to understanding the structures of many biological network systems.
26223829	3	3	theme	minimal	512:518	arg1	strength					526:533	minimal ionic strength	512:533	minimal ionic strength	512:533	The studies were conducted at pH 4 at minimal ionic strength, where the βLgA and the pectins are oppositely charged, resulting in an electrostatic attraction to each other.
26223829	0	4	theme	pectin	87:92	arg1	interactions					94:105	pectin interactions	87:105	pectin interactions	87:105	Structural mechanism of complex assemblies: characterisation of beta-lactoglobulin and pectin interactions.
26223829	3	5	theme	ionic	520:524	arg1	strength					526:533	minimal ionic strength	512:533	minimal ionic strength	512:533	The studies were conducted at pH 4 at minimal ionic strength, where the βLgA and the pectins are oppositely charged, resulting in an electrostatic attraction to each other.
26223829	7	6	theme	primary	1474:1480	arg1	complexes					1482:1490	the primary complexes	1470:1490	the primary complexes mediated by protein-rich domains	1470:1523	An increase in the turbidity of the solution of the resultant complexes indicates the formation of large-scale interpolymer associations of the primary complexes mediated by protein-rich domains.
26223829	5	7	with	pectins	828:834	arg1	charge					863:868	charge	863:868	charge	863:868	It was found that βLgA only interacted with pectins with an adequate amount of charge, and that the complexation between βLgA and pectin was a two-step process initially involving binding of the protein to available sites on the pectin, and subsequently binding of the protein onto the bound protein that has previously adsorbed.
26223829	5	7	with	pectins	828:834	arg1	amount					853:858	an adequate amount	841:858	an adequate amount of charge	841:868	It was found that βLgA only interacted with pectins with an adequate amount of charge, and that the complexation between βLgA and pectin was a two-step process initially involving binding of the protein to available sites on the pectin, and subsequently binding of the protein onto the bound protein that has previously adsorbed.
26223829	6	8	theme	intrinsic	1142:1150	arg1	fluorescence					1163:1174	intrinsic tryptophan fluorescence	1142:1174	intrinsic tryptophan fluorescence	1142:1174	Circular dichroism (CD) and intrinsic tryptophan fluorescence were also measured of βLgA during its interaction with the pectin samples, and show that the binding leads to significant conformational changes in βLgA.
26223829	7	9	theme	associations	1454:1465	arg1	formation					1416:1424	the formation	1412:1424	the formation of large-scale interpolymer associations of the primary complexes mediated by protein-rich domains	1412:1523	An increase in the turbidity of the solution of the resultant complexes indicates the formation of large-scale interpolymer associations of the primary complexes mediated by protein-rich domains.
26223829	5	10	dep	process	936:942	arg1	involving					954:962	involving	954:962	initially involving binding of the protein to available sites on the pectin	944:1018	It was found that βLgA only interacted with pectins with an adequate amount of charge, and that the complexation between βLgA and pectin was a two-step process initially involving binding of the protein to available sites on the pectin, and subsequently binding of the protein onto the bound protein that has previously adsorbed.
26223829	5	10	dep	process	936:942	arg1	binding					1038:1044	binding	1038:1044	binding of the protein onto the bound protein that has previously adsorbed	1038:1111	It was found that βLgA only interacted with pectins with an adequate amount of charge, and that the complexation between βLgA and pectin was a two-step process initially involving binding of the protein to available sites on the pectin, and subsequently binding of the protein onto the bound protein that has previously adsorbed.
26223829	1	11	theme	many	301:304	arg1	systems					325:331	many biological network systems	301:331	many biological network systems	301:331	Knowledge of how proteins and polysaccharides interact is the key to understanding encapsulation and emulsification in these composite systems and ultimately to understanding the structures of many biological network systems.
26223829	5	12	theme	protein	979:985	arg1	binding					964:970	binding	964:970	binding of the protein to available sites on the pectin	964:1018	It was found that βLgA only interacted with pectins with an adequate amount of charge, and that the complexation between βLgA and pectin was a two-step process initially involving binding of the protein to available sites on the pectin, and subsequently binding of the protein onto the bound protein that has previously adsorbed.
26223829	7	13	from	increase	1333:1340	arg1	turbidity					1349:1357	the turbidity	1345:1357	the turbidity of the solution of the resultant complexes	1345:1400	An increase in the turbidity of the solution of the resultant complexes indicates the formation of large-scale interpolymer associations of the primary complexes mediated by protein-rich domains.
26223829	4	14	theme	calorimetry	668:678	arg1	experiments					686:696	Isothermal titration calorimetry (ITC) experiments	647:696	Isothermal titration calorimetry (ITC) experiments	647:696	Isothermal titration calorimetry (ITC) experiments were performed to determine the thermodynamics associated with βLgA-pectin titration.
26223829	7	15	theme	large-scale	1429:1439	arg1	associations					1454:1465	large-scale interpolymer associations	1429:1465	large-scale interpolymer associations of the primary complexes mediated by protein-rich domains	1429:1523	An increase in the turbidity of the solution of the resultant complexes indicates the formation of large-scale interpolymer associations of the primary complexes mediated by protein-rich domains.
26223829	5	16	theme	adequate	844:851	arg1	charge					863:868	charge	863:868	charge	863:868	It was found that βLgA only interacted with pectins with an adequate amount of charge, and that the complexation between βLgA and pectin was a two-step process initially involving binding of the protein to available sites on the pectin, and subsequently binding of the protein onto the bound protein that has previously adsorbed.
26223829	5	16	theme	adequate	844:851	arg1	amount					853:858	an adequate amount	841:858	an adequate amount of charge	841:868	It was found that βLgA only interacted with pectins with an adequate amount of charge, and that the complexation between βLgA and pectin was a two-step process initially involving binding of the protein to available sites on the pectin, and subsequently binding of the protein onto the bound protein that has previously adsorbed.
26223829	2	17	theme	charge	466:471	arg1	pectins					410:416	pectins	410:416	pectins of various amounts	410:435	As a model system we have studied β-lactoglobulin A (βLgA) interacting with pectins of various amounts and distribution patterns of charge.
26223829	2	17	theme	charge	466:471	arg1	patterns					454:461	distribution patterns	441:461	distribution patterns of charge	441:471	As a model system we have studied β-lactoglobulin A (βLgA) interacting with pectins of various amounts and distribution patterns of charge.
26223829	4	18	theme	titration	658:666	arg1	calorimetry					668:678	Isothermal titration calorimetry	647:678	Isothermal titration calorimetry (ITC) experiments	647:696	Isothermal titration calorimetry (ITC) experiments were performed to determine the thermodynamics associated with βLgA-pectin titration.
26223829	4	18	theme	titration	658:666	arg1	ITC					681:683	ITC	681:683	ITC	681:683	Isothermal titration calorimetry (ITC) experiments were performed to determine the thermodynamics associated with βLgA-pectin titration.
26223829	7	19	theme	interpolymer	1441:1452	arg1	associations					1454:1465	large-scale interpolymer associations	1429:1465	large-scale interpolymer associations of the primary complexes mediated by protein-rich domains	1429:1523	An increase in the turbidity of the solution of the resultant complexes indicates the formation of large-scale interpolymer associations of the primary complexes mediated by protein-rich domains.
26223829	1	20	dep	key	170:172	arg1	understanding					177:189	understanding	177:189	to understanding encapsulation and emulsification in these composite systems	174:249	Knowledge of how proteins and polysaccharides interact is the key to understanding encapsulation and emulsification in these composite systems and ultimately to understanding the structures of many biological network systems.
26223829	1	20	dep	key	170:172	arg1	understanding					269:281	understanding	269:281	ultimately to understanding the structures of many biological network systems	255:331	Knowledge of how proteins and polysaccharides interact is the key to understanding encapsulation and emulsification in these composite systems and ultimately to understanding the structures of many biological network systems.
26223829	5	21	from	sites	1000:1004	arg1	pectin					1013:1018	the pectin	1009:1018	the pectin	1009:1018	It was found that βLgA only interacted with pectins with an adequate amount of charge, and that the complexation between βLgA and pectin was a two-step process initially involving binding of the protein to available sites on the pectin, and subsequently binding of the protein onto the bound protein that has previously adsorbed.
26223829	4	22	theme	Isothermal	647:656	arg1	calorimetry					668:678	Isothermal titration calorimetry	647:678	Isothermal titration calorimetry (ITC) experiments	647:696	Isothermal titration calorimetry (ITC) experiments were performed to determine the thermodynamics associated with βLgA-pectin titration.
26223829	4	22	theme	Isothermal	647:656	arg1	ITC					681:683	ITC	681:683	ITC	681:683	Isothermal titration calorimetry (ITC) experiments were performed to determine the thermodynamics associated with βLgA-pectin titration.
26223829	0	23	theme	Structural	0:9	arg1	mechanism					11:19	Structural mechanism	0:19	Structural mechanism of complex assemblies: characterisation of beta-lactoglobulin and pectin interactions.	0:106	Structural mechanism of complex assemblies: characterisation of beta-lactoglobulin and pectin interactions.
26223829	1	24	theme	biological	306:315	arg1	systems					325:331	many biological network systems	301:331	many biological network systems	301:331	Knowledge of how proteins and polysaccharides interact is the key to understanding encapsulation and emulsification in these composite systems and ultimately to understanding the structures of many biological network systems.
26223829	5	25	theme	protein	1053:1059	arg1	binding					1038:1044	binding	1038:1044	binding of the protein onto the bound protein that has previously adsorbed	1038:1111	It was found that βLgA only interacted with pectins with an adequate amount of charge, and that the complexation between βLgA and pectin was a two-step process initially involving binding of the protein to available sites on the pectin, and subsequently binding of the protein onto the bound protein that has previously adsorbed.
26223829	2	26	theme	model	339:343	arg1	A					384:384	β-lactoglobulin A	368:384	β-lactoglobulin A (βLgA) interacting with pectins of various amounts and distribution patterns of charge	368:471	As a model system we have studied β-lactoglobulin A (βLgA) interacting with pectins of various amounts and distribution patterns of charge.
26223829	2	26	theme	model	339:343	arg1	system					345:350	a model system	337:350	a model system	337:350	As a model system we have studied β-lactoglobulin A (βLgA) interacting with pectins of various amounts and distribution patterns of charge.
26223829	0	27	theme	complex	24:30	arg1	assemblies					32:41	complex assemblies	24:41	complex assemblies	24:41	Structural mechanism of complex assemblies: characterisation of beta-lactoglobulin and pectin interactions.
26223829	1	28	theme	network	317:323	arg1	systems					325:331	many biological network systems	301:331	many biological network systems	301:331	Knowledge of how proteins and polysaccharides interact is the key to understanding encapsulation and emulsification in these composite systems and ultimately to understanding the structures of many biological network systems.
26223829	2	29	theme	distribution	441:452	arg1	patterns					454:461	distribution patterns	441:461	distribution patterns of charge	441:471	As a model system we have studied β-lactoglobulin A (βLgA) interacting with pectins of various amounts and distribution patterns of charge.
26223829	7	30	theme	complexes	1392:1400	arg1	solution					1366:1373	the solution	1362:1373	the solution of the resultant complexes	1362:1400	An increase in the turbidity of the solution of the resultant complexes indicates the formation of large-scale interpolymer associations of the primary complexes mediated by protein-rich domains.
26223829	1	31	theme	systems	325:331	arg1	structures					287:296	the structures	283:296	the structures of many biological network systems	283:331	Knowledge of how proteins and polysaccharides interact is the key to understanding encapsulation and emulsification in these composite systems and ultimately to understanding the structures of many biological network systems.
26223829	4	32	theme	βLgA-pectin	761:771	arg1	titration					773:781	βLgA-pectin titration	761:781	βLgA-pectin titration	761:781	Isothermal titration calorimetry (ITC) experiments were performed to determine the thermodynamics associated with βLgA-pectin titration.
26223829	1	33	theme	composite	233:241	arg1	systems					243:249	these composite systems	227:249	these composite systems	227:249	Knowledge of how proteins and polysaccharides interact is the key to understanding encapsulation and emulsification in these composite systems and ultimately to understanding the structures of many biological network systems.
26223829	2	34	theme	amounts	429:435	arg1	pectins					410:416	pectins	410:416	pectins of various amounts	410:435	As a model system we have studied β-lactoglobulin A (βLgA) interacting with pectins of various amounts and distribution patterns of charge.
26223829	2	34	theme	amounts	429:435	arg1	patterns					454:461	distribution patterns	441:461	distribution patterns of charge	441:471	As a model system we have studied β-lactoglobulin A (βLgA) interacting with pectins of various amounts and distribution patterns of charge.
26223829	0	35	theme	assemblies	32:41	arg1	mechanism					11:19	Structural mechanism	0:19	Structural mechanism of complex assemblies: characterisation of beta-lactoglobulin and pectin interactions.	0:106	Structural mechanism of complex assemblies: characterisation of beta-lactoglobulin and pectin interactions.
26223829	5	36	theme	bound	1070:1074	arg1	protein					1076:1082	the bound protein	1066:1082	the bound protein that has previously adsorbed	1066:1111	It was found that βLgA only interacted with pectins with an adequate amount of charge, and that the complexation between βLgA and pectin was a two-step process initially involving binding of the protein to available sites on the pectin, and subsequently binding of the protein onto the bound protein that has previously adsorbed.
26223829	7	37	theme	solution	1366:1373	arg1	turbidity					1349:1357	the turbidity	1345:1357	the turbidity of the solution of the resultant complexes	1345:1400	An increase in the turbidity of the solution of the resultant complexes indicates the formation of large-scale interpolymer associations of the primary complexes mediated by protein-rich domains.
26223829	2	38	theme	various	421:427	arg1	amounts					429:435	various amounts	421:435	various amounts	421:435	As a model system we have studied β-lactoglobulin A (βLgA) interacting with pectins of various amounts and distribution patterns of charge.
26223829	6	39	theme	conformational	1298:1311	arg1	changes					1313:1319	significant conformational changes	1286:1319	significant conformational changes in βLgA	1286:1327	Circular dichroism (CD) and intrinsic tryptophan fluorescence were also measured of βLgA during its interaction with the pectin samples, and show that the binding leads to significant conformational changes in βLgA.
26223829	6	40	from	changes	1313:1319	arg1	βLgA					1324:1327	βLgA	1324:1327	βLgA	1324:1327	Circular dichroism (CD) and intrinsic tryptophan fluorescence were also measured of βLgA during its interaction with the pectin samples, and show that the binding leads to significant conformational changes in βLgA.
26223829	2	41	theme	β-lactoglobulin	368:382	arg1	βLgA					387:390	βLgA	387:390	βLgA	387:390	As a model system we have studied β-lactoglobulin A (βLgA) interacting with pectins of various amounts and distribution patterns of charge.
26223829	2	41	theme	β-lactoglobulin	368:382	arg1	A					384:384	β-lactoglobulin A	368:384	β-lactoglobulin A (βLgA) interacting with pectins of various amounts and distribution patterns of charge	368:471	As a model system we have studied β-lactoglobulin A (βLgA) interacting with pectins of various amounts and distribution patterns of charge.
26223829	2	41	theme	β-lactoglobulin	368:382	arg1	system					345:350	a model system	337:350	a model system	337:350	As a model system we have studied β-lactoglobulin A (βLgA) interacting with pectins of various amounts and distribution patterns of charge.
26223829	7	42	theme	resultant	1382:1390	arg1	complexes					1392:1400	the resultant complexes	1378:1400	the resultant complexes	1378:1400	An increase in the turbidity of the solution of the resultant complexes indicates the formation of large-scale interpolymer associations of the primary complexes mediated by protein-rich domains.
26223829	6	43	theme	significant	1286:1296	arg1	changes					1313:1319	significant conformational changes	1286:1319	significant conformational changes in βLgA	1286:1327	Circular dichroism (CD) and intrinsic tryptophan fluorescence were also measured of βLgA during its interaction with the pectin samples, and show that the binding leads to significant conformational changes in βLgA.
26223829	5	44	theme	available	990:998	arg1	sites					1000:1004	available sites	990:1004	available sites on the pectin	990:1018	It was found that βLgA only interacted with pectins with an adequate amount of charge, and that the complexation between βLgA and pectin was a two-step process initially involving binding of the protein to available sites on the pectin, and subsequently binding of the protein onto the bound protein that has previously adsorbed.
26223829	6	45	theme	Circular	1114:1121	arg1	CD					1134:1135	CD	1134:1135	CD	1134:1135	Circular dichroism (CD) and intrinsic tryptophan fluorescence were also measured of βLgA during its interaction with the pectin samples, and show that the binding leads to significant conformational changes in βLgA.
26223829	6	45	theme	Circular	1114:1121	arg1	dichroism					1123:1131	Circular dichroism	1114:1131	Circular dichroism (CD)	1114:1136	Circular dichroism (CD) and intrinsic tryptophan fluorescence were also measured of βLgA during its interaction with the pectin samples, and show that the binding leads to significant conformational changes in βLgA.
26223829	6	46	with	interaction	1214:1224	arg1	samples					1242:1248	the pectin samples	1231:1248	the pectin samples	1231:1248	Circular dichroism (CD) and intrinsic tryptophan fluorescence were also measured of βLgA during its interaction with the pectin samples, and show that the binding leads to significant conformational changes in βLgA.
26223829	6	47	theme	tryptophan	1152:1161	arg1	fluorescence					1163:1174	intrinsic tryptophan fluorescence	1142:1174	intrinsic tryptophan fluorescence	1142:1174	Circular dichroism (CD) and intrinsic tryptophan fluorescence were also measured of βLgA during its interaction with the pectin samples, and show that the binding leads to significant conformational changes in βLgA.
26223829	5	48	theme	two-step	927:934	arg1	process					936:942	a two-step process	925:942	a two-step process initially involving binding of the protein to available sites on the pectin, and subsequently binding of the protein onto the bound protein that has previously adsorbed	925:1111	It was found that βLgA only interacted with pectins with an adequate amount of charge, and that the complexation between βLgA and pectin was a two-step process initially involving binding of the protein to available sites on the pectin, and subsequently binding of the protein onto the bound protein that has previously adsorbed.
26223829	5	48	theme	two-step	927:934	arg1	complexation					884:895	the complexation	880:895	the complexation between βLgA and pectin	880:919	It was found that βLgA only interacted with pectins with an adequate amount of charge, and that the complexation between βLgA and pectin was a two-step process initially involving binding of the protein to available sites on the pectin, and subsequently binding of the protein onto the bound protein that has previously adsorbed.
26223829	5	49	theme	charge	863:868	arg1	charge					863:868	charge	863:868	charge	863:868	It was found that βLgA only interacted with pectins with an adequate amount of charge, and that the complexation between βLgA and pectin was a two-step process initially involving binding of the protein to available sites on the pectin, and subsequently binding of the protein onto the bound protein that has previously adsorbed.
26223829	5	49	theme	charge	863:868	arg1	amount					853:858	an adequate amount	841:858	an adequate amount of charge	841:868	It was found that βLgA only interacted with pectins with an adequate amount of charge, and that the complexation between βLgA and pectin was a two-step process initially involving binding of the protein to available sites on the pectin, and subsequently binding of the protein onto the bound protein that has previously adsorbed.
26223829	0	50	dep	mechanism	11:19	arg1	characterisation					44:59	characterisation	44:59	Structural mechanism of complex assemblies: characterisation of beta-lactoglobulin and pectin interactions.	0:106	Structural mechanism of complex assemblies: characterisation of beta-lactoglobulin and pectin interactions.
26223829	1	51	from	emulsification	209:222	arg1	systems					243:249	these composite systems	227:249	these composite systems	227:249	Knowledge of how proteins and polysaccharides interact is the key to understanding encapsulation and emulsification in these composite systems and ultimately to understanding the structures of many biological network systems.
26223829	0	52	theme	beta-lactoglobulin	64:81	arg1	characterisation					44:59	characterisation	44:59	Structural mechanism of complex assemblies: characterisation of beta-lactoglobulin and pectin interactions.	0:106	Structural mechanism of complex assemblies: characterisation of beta-lactoglobulin and pectin interactions.
26223829	7	53	theme	complexes	1482:1490	arg1	associations					1454:1465	large-scale interpolymer associations	1429:1465	large-scale interpolymer associations of the primary complexes mediated by protein-rich domains	1429:1523	An increase in the turbidity of the solution of the resultant complexes indicates the formation of large-scale interpolymer associations of the primary complexes mediated by protein-rich domains.
26223829	3	54	theme	electrostatic	607:619	arg1	attraction					621:630	an electrostatic attraction	604:630	an electrostatic attraction to each other	604:644	The studies were conducted at pH 4 at minimal ionic strength, where the βLgA and the pectins are oppositely charged, resulting in an electrostatic attraction to each other.
26223829	7	55	theme	protein-rich	1504:1515	arg1	domains					1517:1523	protein-rich domains	1504:1523	protein-rich domains	1504:1523	An increase in the turbidity of the solution of the resultant complexes indicates the formation of large-scale interpolymer associations of the primary complexes mediated by protein-rich domains.
28198631	3	0	theme	robust	576:581	arg1	films					597:601	robust free-standing films	576:601	robust free-standing films with asymmetric composition, stimuli-responsiveness, and self-healing properties	576:682	On the basis of these screening results, we have found that alginate grafted with catechol groups allows the formation of robust free-standing films with asymmetric composition, stimuli-responsiveness, and self-healing properties.
28198631	6	1	theme	biomedical	1079:1088	arg1	field					1090:1094	the biomedical field	1075:1094	the biomedical field	1075:1094	These results open the door to potential applications in the biomedical field.
28198631	2	2	theme	free-standing	393:405	arg1	membranes					407:415	PDA-composite free-standing membranes	379:415	PDA-composite free-standing membranes produced at the water/air interface	379:451	The use of either small molecules (urea, pyrocatechol) or polymers paves the way to understand which kind of compounds can be used for the formation of PDA-composite free-standing membranes produced at the water/air interface.
28198631	6	3	from	door	1041:1044	arg1	field					1090:1094	the biomedical field	1075:1094	the biomedical field	1075:1094	These results open the door to potential applications in the biomedical field.
28198631	1	4	theme	different	184:192	arg1	strategies					203:212	different chemical strategies	184:212	different chemical strategies	184:212	The formation of polydopamine composite membranes at the water/air interface using different chemical strategies is reported.
28198631	2	5	used	used	353:356	arg2	kind					328:331	kind	328:331	kind of compounds	328:344	The use of either small molecules (urea, pyrocatechol) or polymers paves the way to understand which kind of compounds can be used for the formation of PDA-composite free-standing membranes produced at the water/air interface.
28198631	3	6	theme	catechol	536:543	arg1	groups					545:550	catechol groups	536:550	catechol groups	536:550	On the basis of these screening results, we have found that alginate grafted with catechol groups allows the formation of robust free-standing films with asymmetric composition, stimuli-responsiveness, and self-healing properties.
28198631	4	7	theme	adhesion	761:768	arg1	behavior					770:777	its adhesion behavior	757:777	its adhesion behavior on PDMS	757:785	The stickiness of these membranes depends on the relative humidity, and its adhesion behavior on PDMS was characterized using the JKR method.
28198631	5	8	theme	biocompatible	981:993	arg1	partners					1008:1015	fully biocompatible constituting partners	975:1015	fully biocompatible constituting partners	975:1015	Thus, alginate-catechol polydopamine films appear as a new class of PDA composites, mechanically robust through covalent cross-linking and based on fully biocompatible constituting partners.
28198631	5	9	theme	constituting	995:1006	arg1	partners					1008:1015	fully biocompatible constituting partners	975:1015	fully biocompatible constituting partners	975:1015	Thus, alginate-catechol polydopamine films appear as a new class of PDA composites, mechanically robust through covalent cross-linking and based on fully biocompatible constituting partners.
28198631	3	10	dep	results	486:492	arg1	basis					461:465	basis	461:465	basis	461:465	On the basis of these screening results, we have found that alginate grafted with catechol groups allows the formation of robust free-standing films with asymmetric composition, stimuli-responsiveness, and self-healing properties.
28198631	3	10	dep	results	486:492	arg1	the					457:459	the	457:459	the	457:459	On the basis of these screening results, we have found that alginate grafted with catechol groups allows the formation of robust free-standing films with asymmetric composition, stimuli-responsiveness, and self-healing properties.
28198631	3	11	with	films	597:601	arg1	composition					619:629	asymmetric composition	608:629	asymmetric composition	608:629	On the basis of these screening results, we have found that alginate grafted with catechol groups allows the formation of robust free-standing films with asymmetric composition, stimuli-responsiveness, and self-healing properties.
28198631	3	11	with	films	597:601	arg1	properties					673:682	self-healing properties	660:682	self-healing properties	660:682	On the basis of these screening results, we have found that alginate grafted with catechol groups allows the formation of robust free-standing films with asymmetric composition, stimuli-responsiveness, and self-healing properties.
28198631	3	11	with	films	597:601	arg1	stimuli-responsiveness					632:653	stimuli-responsiveness	632:653	stimuli-responsiveness	632:653	On the basis of these screening results, we have found that alginate grafted with catechol groups allows the formation of robust free-standing films with asymmetric composition, stimuli-responsiveness, and self-healing properties.
28198631	4	12	theme	relative	734:741	arg1	humidity					743:750	the relative humidity	730:750	the relative humidity	730:750	The stickiness of these membranes depends on the relative humidity, and its adhesion behavior on PDMS was characterized using the JKR method.
28198631	1	13	from	interface	168:176	arg1	formation					105:113	The formation	101:113	The formation of polydopamine composite membranes at the water/air interface using different chemical strategies	101:212	The formation of polydopamine composite membranes at the water/air interface using different chemical strategies is reported.
28198631	2	14	theme	polymers	285:292	arg1	use					231:233	The use	227:233	The use of either small molecules (urea, pyrocatechol) or polymers	227:292	The use of either small molecules (urea, pyrocatechol) or polymers paves the way to understand which kind of compounds can be used for the formation of PDA-composite free-standing membranes produced at the water/air interface.
28198631	1	15	theme	chemical	194:201	arg1	strategies					203:212	different chemical strategies	184:212	different chemical strategies	184:212	The formation of polydopamine composite membranes at the water/air interface using different chemical strategies is reported.
28198631	4	16	from	behavior	770:777	arg1	PDMS					782:785	PDMS	782:785	PDMS	782:785	The stickiness of these membranes depends on the relative humidity, and its adhesion behavior on PDMS was characterized using the JKR method.
28198631	6	17	theme	potential	1049:1057	arg1	applications					1059:1070	potential applications	1049:1070	potential applications	1049:1070	These results open the door to potential applications in the biomedical field.
28198631	2	18	theme	PDA-composite	379:391	arg1	membranes					407:415	PDA-composite free-standing membranes	379:415	PDA-composite free-standing membranes produced at the water/air interface	379:451	The use of either small molecules (urea, pyrocatechol) or polymers paves the way to understand which kind of compounds can be used for the formation of PDA-composite free-standing membranes produced at the water/air interface.
28198631	0	19	theme	Alginate-Catechol	7:23	arg1	Membranes					52:60	Robust Alginate-Catechol@Polydopamine Free-Standing Membranes	0:60	Robust Alginate-Catechol@Polydopamine Free-Standing Membranes	0:60	Robust Alginate-Catechol@Polydopamine Free-Standing Membranes Obtained from the Water/Air Interface.
28198631	0	20	theme	Robust	0:5	arg1	Membranes					52:60	Robust Alginate-Catechol@Polydopamine Free-Standing Membranes	0:60	Robust Alginate-Catechol@Polydopamine Free-Standing Membranes	0:60	Robust Alginate-Catechol@Polydopamine Free-Standing Membranes Obtained from the Water/Air Interface.
28198631	0	21	theme	Polydopamine	25:36	arg1	Membranes					52:60	Robust Alginate-Catechol@Polydopamine Free-Standing Membranes	0:60	Robust Alginate-Catechol@Polydopamine Free-Standing Membranes	0:60	Robust Alginate-Catechol@Polydopamine Free-Standing Membranes Obtained from the Water/Air Interface.
28198631	5	22	dep	class	886:890	arg1	based					966:970	based	966:970	based on fully biocompatible constituting partners	966:1015	Thus, alginate-catechol polydopamine films appear as a new class of PDA composites, mechanically robust through covalent cross-linking and based on fully biocompatible constituting partners.
28198631	5	22	dep	class	886:890	arg1	robust					924:929	robust	924:929	robust	924:929	Thus, alginate-catechol polydopamine films appear as a new class of PDA composites, mechanically robust through covalent cross-linking and based on fully biocompatible constituting partners.
28198631	5	23	theme	polydopamine	851:862	arg1	films					864:868	alginate-catechol polydopamine films	833:868	alginate-catechol polydopamine films	833:868	Thus, alginate-catechol polydopamine films appear as a new class of PDA composites, mechanically robust through covalent cross-linking and based on fully biocompatible constituting partners.
28198631	2	24	theme	water/air	433:441	arg1	interface					443:451	the water/air interface	429:451	the water/air interface	429:451	The use of either small molecules (urea, pyrocatechol) or polymers paves the way to understand which kind of compounds can be used for the formation of PDA-composite free-standing membranes produced at the water/air interface.
28198631	3	25	theme	self-healing	660:671	arg1	properties					673:682	self-healing properties	660:682	self-healing properties	660:682	On the basis of these screening results, we have found that alginate grafted with catechol groups allows the formation of robust free-standing films with asymmetric composition, stimuli-responsiveness, and self-healing properties.
28198631	1	26	theme	polydopamine	118:129	arg1	membranes					141:149	polydopamine composite membranes	118:149	polydopamine composite membranes	118:149	The formation of polydopamine composite membranes at the water/air interface using different chemical strategies is reported.
28198631	4	27	theme	membranes	709:717	arg1	stickiness					689:698	The stickiness	685:698	The stickiness of these membranes	685:717	The stickiness of these membranes depends on the relative humidity, and its adhesion behavior on PDMS was characterized using the JKR method.
28198631	2	28	theme	compounds	336:344	arg1	kind					328:331	kind	328:331	kind of compounds	328:344	The use of either small molecules (urea, pyrocatechol) or polymers paves the way to understand which kind of compounds can be used for the formation of PDA-composite free-standing membranes produced at the water/air interface.
28198631	3	29	theme	films	597:601	arg1	formation					563:571	the formation	559:571	the formation of robust free-standing films with asymmetric composition, stimuli-responsiveness, and self-healing properties	559:682	On the basis of these screening results, we have found that alginate grafted with catechol groups allows the formation of robust free-standing films with asymmetric composition, stimuli-responsiveness, and self-healing properties.
28198631	0	30	theme	Free-Standing	38:50	arg1	Membranes					52:60	Robust Alginate-Catechol@Polydopamine Free-Standing Membranes	0:60	Robust Alginate-Catechol@Polydopamine Free-Standing Membranes	0:60	Robust Alginate-Catechol@Polydopamine Free-Standing Membranes Obtained from the Water/Air Interface.
28198631	2	31	theme	molecules	251:259	arg1	use					231:233	The use	227:233	The use of either small molecules (urea, pyrocatechol) or polymers	227:292	The use of either small molecules (urea, pyrocatechol) or polymers paves the way to understand which kind of compounds can be used for the formation of PDA-composite free-standing membranes produced at the water/air interface.
28198631	2	32	theme	membranes	407:415	arg1	formation					366:374	the formation	362:374	the formation of PDA-composite free-standing membranes produced at the water/air interface	362:451	The use of either small molecules (urea, pyrocatechol) or polymers paves the way to understand which kind of compounds can be used for the formation of PDA-composite free-standing membranes produced at the water/air interface.
28198631	5	33	theme	PDA	895:897	arg1	composites					899:908	PDA composites	895:908	PDA composites	895:908	Thus, alginate-catechol polydopamine films appear as a new class of PDA composites, mechanically robust through covalent cross-linking and based on fully biocompatible constituting partners.
28198631	2	34	theme	small	245:249	arg1	molecules					251:259	small molecules	245:259	small molecules (urea, pyrocatechol)	245:280	The use of either small molecules (urea, pyrocatechol) or polymers paves the way to understand which kind of compounds can be used for the formation of PDA-composite free-standing membranes produced at the water/air interface.
28198631	1	35	theme	composite	131:139	arg1	membranes					141:149	polydopamine composite membranes	118:149	polydopamine composite membranes	118:149	The formation of polydopamine composite membranes at the water/air interface using different chemical strategies is reported.
28198631	3	36	theme	free-standing	583:595	arg1	films					597:601	robust free-standing films	576:601	robust free-standing films with asymmetric composition, stimuli-responsiveness, and self-healing properties	576:682	On the basis of these screening results, we have found that alginate grafted with catechol groups allows the formation of robust free-standing films with asymmetric composition, stimuli-responsiveness, and self-healing properties.
28198631	1	37	theme	membranes	141:149	arg1	formation					105:113	The formation	101:113	The formation of polydopamine composite membranes at the water/air interface using different chemical strategies	101:212	The formation of polydopamine composite membranes at the water/air interface using different chemical strategies is reported.
28198631	4	38	theme	JKR	815:817	arg1	method					819:824	the JKR method	811:824	the JKR method	811:824	The stickiness of these membranes depends on the relative humidity, and its adhesion behavior on PDMS was characterized using the JKR method.
28198631	3	39	theme	screening	476:484	arg1	results					486:492	these screening results	470:492	these screening results	470:492	On the basis of these screening results, we have found that alginate grafted with catechol groups allows the formation of robust free-standing films with asymmetric composition, stimuli-responsiveness, and self-healing properties.
28198631	5	40	theme	alginate-catechol	833:849	arg1	films					864:868	alginate-catechol polydopamine films	833:868	alginate-catechol polydopamine films	833:868	Thus, alginate-catechol polydopamine films appear as a new class of PDA composites, mechanically robust through covalent cross-linking and based on fully biocompatible constituting partners.
28198631	5	41	theme	covalent	939:946	arg1	cross-linking					948:960	covalent cross-linking	939:960	covalent cross-linking	939:960	Thus, alginate-catechol polydopamine films appear as a new class of PDA composites, mechanically robust through covalent cross-linking and based on fully biocompatible constituting partners.
28198631	0	42	theme	Water/Air	80:88	arg1	Interface					90:98	the Water/Air Interface	76:98	the Water/Air Interface	76:98	Robust Alginate-Catechol@Polydopamine Free-Standing Membranes Obtained from the Water/Air Interface.
28198631	2	43	dep	molecules	251:259	arg1	pyrocatechol					268:279	pyrocatechol	268:279	pyrocatechol	268:279	The use of either small molecules (urea, pyrocatechol) or polymers paves the way to understand which kind of compounds can be used for the formation of PDA-composite free-standing membranes produced at the water/air interface.
28198631	2	43	dep	molecules	251:259	arg1	urea					262:265	urea	262:265	urea	262:265	The use of either small molecules (urea, pyrocatechol) or polymers paves the way to understand which kind of compounds can be used for the formation of PDA-composite free-standing membranes produced at the water/air interface.
28198631	3	44	theme	asymmetric	608:617	arg1	composition					619:629	asymmetric composition	608:629	asymmetric composition	608:629	On the basis of these screening results, we have found that alginate grafted with catechol groups allows the formation of robust free-standing films with asymmetric composition, stimuli-responsiveness, and self-healing properties.
28198631	1	45	theme	water/air	158:166	arg1	interface					168:176	the water/air interface	154:176	the water/air interface using different chemical strategies	154:212	The formation of polydopamine composite membranes at the water/air interface using different chemical strategies is reported.
28198631	5	46	theme	new	882:884	arg1	class					886:890	a new class	880:890	a new class	880:890	Thus, alginate-catechol polydopamine films appear as a new class of PDA composites, mechanically robust through covalent cross-linking and based on fully biocompatible constituting partners.
28198631	0	47	theme	@	24:24	arg1	Membranes					52:60	Robust Alginate-Catechol@Polydopamine Free-Standing Membranes	0:60	Robust Alginate-Catechol@Polydopamine Free-Standing Membranes	0:60	Robust Alginate-Catechol@Polydopamine Free-Standing Membranes Obtained from the Water/Air Interface.
28198631	5	48	theme	composites	899:908	arg1	class					886:890	a new class	880:890	a new class	880:890	Thus, alginate-catechol polydopamine films appear as a new class of PDA composites, mechanically robust through covalent cross-linking and based on fully biocompatible constituting partners.
24352860	2	0	theme	EPS	407:409	arg1	fraction					411:418	A purified EPS fraction	396:418	A purified EPS fraction	396:418	A purified EPS fraction was attained from gel filtration chromatography and its major molecular characteristics were determined.
24352860	1	1	theme	g/L	329:331	arg1	maltose					333:339	50 g/L maltose	326:339	50 g/L maltose	326:339	The major nutrients, pH and temperature were evaluated for the exopolysaccharide (EPS) production by Daedalea dickinsii in submerged culture to derive an optimal medium composition and conditions as follows: 50 g/L maltose, 5 g/L soy peptone, 5 mM CaCl(2), at pH 6.0 and 28 °C.
24352860	5	2	theme	thermogravimetric	725:741	arg1	analysis					743:750	thermogravimetric analysis	725:750	thermogravimetric analysis	725:750	Furthermore, thermogravimetric analysis indicated its degradation temperature (T(d)) of 189 °C.
24352860	2	3	theme	molecular	482:490	arg1	characteristics					492:506	its major molecular characteristics	472:506	its major molecular characteristics	472:506	A purified EPS fraction was attained from gel filtration chromatography and its major molecular characteristics were determined.
24352860	1	4	dep	follows	317:323	arg1	maltose					333:339	50 g/L maltose	326:339	50 g/L maltose	326:339	The major nutrients, pH and temperature were evaluated for the exopolysaccharide (EPS) production by Daedalea dickinsii in submerged culture to derive an optimal medium composition and conditions as follows: 50 g/L maltose, 5 g/L soy peptone, 5 mM CaCl(2), at pH 6.0 and 28 °C.
24352860	2	5	theme	purified	398:405	arg1	fraction					411:418	A purified EPS fraction	396:418	A purified EPS fraction	396:418	A purified EPS fraction was attained from gel filtration chromatography and its major molecular characteristics were determined.
24352860	6	6	theme	antioxidant	812:822	arg1	activity					824:831	The antioxidant activity	808:831	The antioxidant activity of the EPS fraction	808:851	The antioxidant activity of the EPS fraction showed a correlation with the molecular properties.
24352860	0	7	theme	activity	108:115	arg1	culture					58:64	submerged culture	48:64	submerged culture of Daedalea dickinsii and its antioxidant activity	48:115	Optimization of exopolysaccharide production in submerged culture of Daedalea dickinsii and its antioxidant activity.
24352860	0	8	from	Optimization	0:11	arg1	culture					58:64	submerged culture	48:64	submerged culture of Daedalea dickinsii and its antioxidant activity	48:115	Optimization of exopolysaccharide production in submerged culture of Daedalea dickinsii and its antioxidant activity.
24352860	6	9	theme	EPS	840:842	arg1	fraction					844:851	the EPS fraction	836:851	the EPS fraction	836:851	The antioxidant activity of the EPS fraction showed a correlation with the molecular properties.
24352860	0	10	theme	antioxidant	96:106	arg1	activity					108:115	its antioxidant activity	92:115	its antioxidant activity	92:115	Optimization of exopolysaccharide production in submerged culture of Daedalea dickinsii and its antioxidant activity.
24352860	1	11	theme	5	342:342	arg1	g/L					344:346	g/L	344:346	g/L	344:346	The major nutrients, pH and temperature were evaluated for the exopolysaccharide (EPS) production by Daedalea dickinsii in submerged culture to derive an optimal medium composition and conditions as follows: 50 g/L maltose, 5 g/L soy peptone, 5 mM CaCl(2), at pH 6.0 and 28 °C.
24352860	7	12	theme	stable	1034:1039	arg1	form					1041:1044	a more stable form	1027:1044	a more stable form	1027:1044	It might be attributed to the functional groups in the EPS fraction, which can donate electrons to reduce the radicals to a more stable form or react with the free radicals to terminate the radical chain reaction.
24352860	7	13	theme	radical	1095:1101	arg1	reaction					1109:1116	the radical chain reaction	1091:1116	the radical chain reaction	1091:1116	It might be attributed to the functional groups in the EPS fraction, which can donate electrons to reduce the radicals to a more stable form or react with the free radicals to terminate the radical chain reaction.
24352860	1	14	theme	g/L	344:346	arg1	follows					317:323	follows	317:323	follows	317:323	The major nutrients, pH and temperature were evaluated for the exopolysaccharide (EPS) production by Daedalea dickinsii in submerged culture to derive an optimal medium composition and conditions as follows: 50 g/L maltose, 5 g/L soy peptone, 5 mM CaCl(2), at pH 6.0 and 28 °C.
24352860	1	14	theme	g/L	344:346	arg1	peptone					352:358	5 g/L soy peptone	342:358	5 g/L soy peptone	342:358	The major nutrients, pH and temperature were evaluated for the exopolysaccharide (EPS) production by Daedalea dickinsii in submerged culture to derive an optimal medium composition and conditions as follows: 50 g/L maltose, 5 g/L soy peptone, 5 mM CaCl(2), at pH 6.0 and 28 °C.
24352860	4	15	theme	GC	618:619	arg1	analysis					621:628	GC analysis	618:628	GC analysis	618:628	GC analysis and NMR spectrum showed its major molecular composition of glucose and galactose.
24352860	7	16	theme	chain	1103:1107	arg1	reaction					1109:1116	the radical chain reaction	1091:1116	the radical chain reaction	1091:1116	It might be attributed to the functional groups in the EPS fraction, which can donate electrons to reduce the radicals to a more stable form or react with the free radicals to terminate the radical chain reaction.
24352860	1	17	theme	soy	348:350	arg1	follows					317:323	follows	317:323	follows	317:323	The major nutrients, pH and temperature were evaluated for the exopolysaccharide (EPS) production by Daedalea dickinsii in submerged culture to derive an optimal medium composition and conditions as follows: 50 g/L maltose, 5 g/L soy peptone, 5 mM CaCl(2), at pH 6.0 and 28 °C.
24352860	1	17	theme	soy	348:350	arg1	peptone					352:358	5 g/L soy peptone	342:358	5 g/L soy peptone	342:358	The major nutrients, pH and temperature were evaluated for the exopolysaccharide (EPS) production by Daedalea dickinsii in submerged culture to derive an optimal medium composition and conditions as follows: 50 g/L maltose, 5 g/L soy peptone, 5 mM CaCl(2), at pH 6.0 and 28 °C.
24352860	5	18	dep	temperature	778:788	arg1	T					791:791	T	791:791	T(d)	791:794	Furthermore, thermogravimetric analysis indicated its degradation temperature (T(d)) of 189 °C.
24352860	5	18	dep	temperature	778:788	arg1	d					793:793	d	793:793	d	793:793	Furthermore, thermogravimetric analysis indicated its degradation temperature (T(d)) of 189 °C.
24352860	1	19	theme	exopolysaccharide	181:197	arg1	production					205:214	the exopolysaccharide (EPS) production	177:214	the exopolysaccharide (EPS) production by Daedalea dickinsii in submerged culture to derive an optimal medium composition and conditions as follows: 50 g/L maltose, 5 g/L soy peptone, 5 mM CaCl(2), at pH 6.0 and 28 °C	177:393	The major nutrients, pH and temperature were evaluated for the exopolysaccharide (EPS) production by Daedalea dickinsii in submerged culture to derive an optimal medium composition and conditions as follows: 50 g/L maltose, 5 g/L soy peptone, 5 mM CaCl(2), at pH 6.0 and 28 °C.
24352860	1	20	theme	optimal	272:278	arg1	composition					287:297	an optimal medium composition	269:297	an optimal medium composition	269:297	The major nutrients, pH and temperature were evaluated for the exopolysaccharide (EPS) production by Daedalea dickinsii in submerged culture to derive an optimal medium composition and conditions as follows: 50 g/L maltose, 5 g/L soy peptone, 5 mM CaCl(2), at pH 6.0 and 28 °C.
24352860	4	21	theme	glucose	689:695	arg1	composition					674:684	its major molecular composition	654:684	its major molecular composition of glucose and galactose	654:709	GC analysis and NMR spectrum showed its major molecular composition of glucose and galactose.
24352860	3	22	theme	alcohols	608:615	arg1	groups					587:592	the prominent characteristic groups	558:592	the prominent characteristic groups of polyhydric alcohols	558:615	FT-IR spectral analysis revealed the prominent characteristic groups of polyhydric alcohols.
24352860	3	22	theme	alcohols	608:615	arg1	alcohols					608:615	polyhydric alcohols	597:615	polyhydric alcohols	597:615	FT-IR spectral analysis revealed the prominent characteristic groups of polyhydric alcohols.
24352860	0	23	theme	production	34:43	arg1	Optimization					0:11	Optimization	0:11	Optimization of exopolysaccharide production in submerged culture of Daedalea dickinsii and its antioxidant activity.	0:116	Optimization of exopolysaccharide production in submerged culture of Daedalea dickinsii and its antioxidant activity.
24352860	1	24	theme	medium	280:285	arg1	composition					287:297	an optimal medium composition	269:297	an optimal medium composition	269:297	The major nutrients, pH and temperature were evaluated for the exopolysaccharide (EPS) production by Daedalea dickinsii in submerged culture to derive an optimal medium composition and conditions as follows: 50 g/L maltose, 5 g/L soy peptone, 5 mM CaCl(2), at pH 6.0 and 28 °C.
24352860	2	25	theme	major	476:480	arg1	characteristics					492:506	its major molecular characteristics	472:506	its major molecular characteristics	472:506	A purified EPS fraction was attained from gel filtration chromatography and its major molecular characteristics were determined.
24352860	7	26	from	groups	946:951	arg1	fraction					964:971	the EPS fraction	956:971	the EPS fraction	956:971	It might be attributed to the functional groups in the EPS fraction, which can donate electrons to reduce the radicals to a more stable form or react with the free radicals to terminate the radical chain reaction.
24352860	0	27	theme	exopolysaccharide	16:32	arg1	production					34:43	exopolysaccharide production	16:43	exopolysaccharide production	16:43	Optimization of exopolysaccharide production in submerged culture of Daedalea dickinsii and its antioxidant activity.
24352860	3	28	theme	polyhydric	597:606	arg1	alcohols					608:615	polyhydric alcohols	597:615	polyhydric alcohols	597:615	FT-IR spectral analysis revealed the prominent characteristic groups of polyhydric alcohols.
24352860	4	29	theme	galactose	701:709	arg1	composition					674:684	its major molecular composition	654:684	its major molecular composition of glucose and galactose	654:709	GC analysis and NMR spectrum showed its major molecular composition of glucose and galactose.
24352860	0	30	theme	submerged	48:56	arg1	culture					58:64	submerged culture	48:64	submerged culture of Daedalea dickinsii and its antioxidant activity	48:115	Optimization of exopolysaccharide production in submerged culture of Daedalea dickinsii and its antioxidant activity.
24352860	1	31	theme	5	361:361	arg1	mM					363:364	mM	363:364	mM	363:364	The major nutrients, pH and temperature were evaluated for the exopolysaccharide (EPS) production by Daedalea dickinsii in submerged culture to derive an optimal medium composition and conditions as follows: 50 g/L maltose, 5 g/L soy peptone, 5 mM CaCl(2), at pH 6.0 and 28 °C.
24352860	3	32	theme	spectral	531:538	arg1	analysis					540:547	FT-IR spectral analysis	525:547	FT-IR spectral analysis	525:547	FT-IR spectral analysis revealed the prominent characteristic groups of polyhydric alcohols.
24352860	5	33	theme	°C	804:805	arg1	temperature					778:788	its degradation temperature	762:788	its degradation temperature (T(d)) of 189 °C	762:805	Furthermore, thermogravimetric analysis indicated its degradation temperature (T(d)) of 189 °C.
24352860	7	34	theme	free	1064:1067	arg1	radicals					1069:1076	the free radicals	1060:1076	the free radicals	1060:1076	It might be attributed to the functional groups in the EPS fraction, which can donate electrons to reduce the radicals to a more stable form or react with the free radicals to terminate the radical chain reaction.
24352860	1	35	theme	mM	363:364	arg1	2					371:371	2	371:371	2	371:371	The major nutrients, pH and temperature were evaluated for the exopolysaccharide (EPS) production by Daedalea dickinsii in submerged culture to derive an optimal medium composition and conditions as follows: 50 g/L maltose, 5 g/L soy peptone, 5 mM CaCl(2), at pH 6.0 and 28 °C.
24352860	1	35	theme	mM	363:364	arg1	CaCl					366:369	5 mM CaCl	361:369	5 mM CaCl(2)	361:372	The major nutrients, pH and temperature were evaluated for the exopolysaccharide (EPS) production by Daedalea dickinsii in submerged culture to derive an optimal medium composition and conditions as follows: 50 g/L maltose, 5 g/L soy peptone, 5 mM CaCl(2), at pH 6.0 and 28 °C.
24352860	1	35	theme	mM	363:364	arg1	follows					317:323	follows	317:323	follows	317:323	The major nutrients, pH and temperature were evaluated for the exopolysaccharide (EPS) production by Daedalea dickinsii in submerged culture to derive an optimal medium composition and conditions as follows: 50 g/L maltose, 5 g/L soy peptone, 5 mM CaCl(2), at pH 6.0 and 28 °C.
24352860	5	36	theme	189	800:802	arg1	°C					804:805	189 °C	800:805	189 °C	800:805	Furthermore, thermogravimetric analysis indicated its degradation temperature (T(d)) of 189 °C.
24352860	5	37	theme	degradation	766:776	arg1	temperature					778:788	its degradation temperature	762:788	its degradation temperature (T(d)) of 189 °C	762:805	Furthermore, thermogravimetric analysis indicated its degradation temperature (T(d)) of 189 °C.
24352860	2	38	theme	filtration	442:451	arg1	chromatography					453:466	gel filtration chromatography	438:466	gel filtration chromatography	438:466	A purified EPS fraction was attained from gel filtration chromatography and its major molecular characteristics were determined.
24352860	2	39	theme	gel	438:440	arg1	chromatography					453:466	gel filtration chromatography	438:466	gel filtration chromatography	438:466	A purified EPS fraction was attained from gel filtration chromatography and its major molecular characteristics were determined.
24352860	7	40	theme	EPS	960:962	arg1	fraction					964:971	the EPS fraction	956:971	the EPS fraction	956:971	It might be attributed to the functional groups in the EPS fraction, which can donate electrons to reduce the radicals to a more stable form or react with the free radicals to terminate the radical chain reaction.
24352860	1	41	theme	major	122:126	arg1	temperature					146:156	temperature	146:156	temperature	146:156	The major nutrients, pH and temperature were evaluated for the exopolysaccharide (EPS) production by Daedalea dickinsii in submerged culture to derive an optimal medium composition and conditions as follows: 50 g/L maltose, 5 g/L soy peptone, 5 mM CaCl(2), at pH 6.0 and 28 °C.
24352860	1	41	theme	major	122:126	arg1	pH					139:140	pH	139:140	pH	139:140	The major nutrients, pH and temperature were evaluated for the exopolysaccharide (EPS) production by Daedalea dickinsii in submerged culture to derive an optimal medium composition and conditions as follows: 50 g/L maltose, 5 g/L soy peptone, 5 mM CaCl(2), at pH 6.0 and 28 °C.
24352860	1	41	theme	major	122:126	arg1	nutrients					128:136	The major nutrients	118:136	The major nutrients	118:136	The major nutrients, pH and temperature were evaluated for the exopolysaccharide (EPS) production by Daedalea dickinsii in submerged culture to derive an optimal medium composition and conditions as follows: 50 g/L maltose, 5 g/L soy peptone, 5 mM CaCl(2), at pH 6.0 and 28 °C.
24352860	6	42	theme	fraction	844:851	arg1	activity					824:831	The antioxidant activity	808:831	The antioxidant activity of the EPS fraction	808:851	The antioxidant activity of the EPS fraction showed a correlation with the molecular properties.
24352860	3	43	theme	FT-IR	525:529	arg1	analysis					540:547	FT-IR spectral analysis	525:547	FT-IR spectral analysis	525:547	FT-IR spectral analysis revealed the prominent characteristic groups of polyhydric alcohols.
24352860	4	44	theme	major	658:662	arg1	composition					674:684	its major molecular composition	654:684	its major molecular composition of glucose and galactose	654:709	GC analysis and NMR spectrum showed its major molecular composition of glucose and galactose.
24352860	4	45	theme	molecular	664:672	arg1	composition					674:684	its major molecular composition	654:684	its major molecular composition of glucose and galactose	654:709	GC analysis and NMR spectrum showed its major molecular composition of glucose and galactose.
24352860	3	46	theme	prominent	562:570	arg1	groups					587:592	the prominent characteristic groups	558:592	the prominent characteristic groups of polyhydric alcohols	558:615	FT-IR spectral analysis revealed the prominent characteristic groups of polyhydric alcohols.
24352860	3	46	theme	prominent	562:570	arg1	alcohols					608:615	polyhydric alcohols	597:615	polyhydric alcohols	597:615	FT-IR spectral analysis revealed the prominent characteristic groups of polyhydric alcohols.
24352860	6	47	with	correlation	862:872	arg1	properties					893:902	the molecular properties	879:902	the molecular properties	879:902	The antioxidant activity of the EPS fraction showed a correlation with the molecular properties.
24352860	1	48	theme	Daedalea	219:226	arg1	dickinsii					228:236	Daedalea dickinsii	219:236	Daedalea dickinsii in submerged culture to derive an optimal medium composition and conditions as follows: 50 g/L maltose, 5 g/L soy peptone, 5 mM CaCl(2), at pH 6.0 and 28 °C	219:393	The major nutrients, pH and temperature were evaluated for the exopolysaccharide (EPS) production by Daedalea dickinsii in submerged culture to derive an optimal medium composition and conditions as follows: 50 g/L maltose, 5 g/L soy peptone, 5 mM CaCl(2), at pH 6.0 and 28 °C.
24352860	1	49	from	dickinsii	228:236	arg1	culture					251:257	submerged culture	241:257	submerged culture	241:257	The major nutrients, pH and temperature were evaluated for the exopolysaccharide (EPS) production by Daedalea dickinsii in submerged culture to derive an optimal medium composition and conditions as follows: 50 g/L maltose, 5 g/L soy peptone, 5 mM CaCl(2), at pH 6.0 and 28 °C.
24352860	3	50	theme	characteristic	572:585	arg1	groups					587:592	the prominent characteristic groups	558:592	the prominent characteristic groups of polyhydric alcohols	558:615	FT-IR spectral analysis revealed the prominent characteristic groups of polyhydric alcohols.
24352860	3	50	theme	characteristic	572:585	arg1	alcohols					608:615	polyhydric alcohols	597:615	polyhydric alcohols	597:615	FT-IR spectral analysis revealed the prominent characteristic groups of polyhydric alcohols.
24352860	0	51	theme	dickinsii	78:86	arg1	culture					58:64	submerged culture	48:64	submerged culture of Daedalea dickinsii and its antioxidant activity	48:115	Optimization of exopolysaccharide production in submerged culture of Daedalea dickinsii and its antioxidant activity.
24352860	7	52	theme	functional	935:944	arg1	groups					946:951	the functional groups	931:951	the functional groups in the EPS fraction, which can donate electrons to reduce the radicals to a more stable form or react with the free radicals to terminate the radical chain reaction	931:1116	It might be attributed to the functional groups in the EPS fraction, which can donate electrons to reduce the radicals to a more stable form or react with the free radicals to terminate the radical chain reaction.
24352860	0	53	theme	Daedalea	69:76	arg1	dickinsii					78:86	Daedalea dickinsii	69:86	Daedalea dickinsii	69:86	Optimization of exopolysaccharide production in submerged culture of Daedalea dickinsii and its antioxidant activity.
24352860	1	54	theme	50	326:327	arg1	g/L					329:331	g/L	329:331	g/L	329:331	The major nutrients, pH and temperature were evaluated for the exopolysaccharide (EPS) production by Daedalea dickinsii in submerged culture to derive an optimal medium composition and conditions as follows: 50 g/L maltose, 5 g/L soy peptone, 5 mM CaCl(2), at pH 6.0 and 28 °C.
24352860	4	55	theme	NMR	634:636	arg1	spectrum					638:645	NMR spectrum	634:645	NMR spectrum	634:645	GC analysis and NMR spectrum showed its major molecular composition of glucose and galactose.
24352860	6	56	theme	molecular	883:891	arg1	properties					893:902	the molecular properties	879:902	the molecular properties	879:902	The antioxidant activity of the EPS fraction showed a correlation with the molecular properties.
24352860	1	57	theme	submerged	241:249	arg1	culture					251:257	submerged culture	241:257	submerged culture	241:257	The major nutrients, pH and temperature were evaluated for the exopolysaccharide (EPS) production by Daedalea dickinsii in submerged culture to derive an optimal medium composition and conditions as follows: 50 g/L maltose, 5 g/L soy peptone, 5 mM CaCl(2), at pH 6.0 and 28 °C.
27044743	5	0	theme	capsular	1187:1194	arg1	production					1211:1220	capsular polysaccharide production	1187:1220	capsular polysaccharide production	1187:1220	Utilizing this approach we identified a group of mutants that all carried mutations in the ATP-binding cassette phosphate transport locus pst These mutants collectively exhibited a strong overproduction of capsular polysaccharides, including α-glucan and arabinomannan, suggestive of a role for inorganic phosphate (Pi) metabolism in modulating capsular polysaccharide production.
27044743	6	1	theme	capsule	1376:1382	arg1	production					1384:1393	capsule production	1376:1393	capsule production	1376:1393	These findings were corroborated by the observation that growth under low Pi conditions as well as chemical activation of the stringent response induces capsule production in a number of mycobacterial species.
27044743	4	2	theme	capsule	730:736	arg1	biosynthesis					738:749	mycobacterial capsule biosynthesis	716:749	mycobacterial capsule biosynthesis	716:749	Here, we study the mechanisms involved in regulation of mycobacterial capsule biosynthesis using a high throughput screen for gene products involved in capsular α-glucan production.
27044743	6	3	theme	mycobacterial	1410:1422	arg1	species					1424:1430	mycobacterial species	1410:1430	mycobacterial species	1410:1430	These findings were corroborated by the observation that growth under low Pi conditions as well as chemical activation of the stringent response induces capsule production in a number of mycobacterial species.
27044743	5	4	theme	polysaccharide	1196:1209	arg1	production					1211:1220	capsular polysaccharide production	1187:1220	capsular polysaccharide production	1187:1220	Utilizing this approach we identified a group of mutants that all carried mutations in the ATP-binding cassette phosphate transport locus pst These mutants collectively exhibited a strong overproduction of capsular polysaccharides, including α-glucan and arabinomannan, suggestive of a role for inorganic phosphate (Pi) metabolism in modulating capsular polysaccharide production.
27044743	5	5	dep	identified	869:878	arg1	Utilizing					842:850	Utilizing	842:850	Utilizing this approach	842:864	Utilizing this approach we identified a group of mutants that all carried mutations in the ATP-binding cassette phosphate transport locus pst These mutants collectively exhibited a strong overproduction of capsular polysaccharides, including α-glucan and arabinomannan, suggestive of a role for inorganic phosphate (Pi) metabolism in modulating capsular polysaccharide production.
27044743	4	6	theme	mycobacterial	716:728	arg1	biosynthesis					738:749	mycobacterial capsule biosynthesis	716:749	mycobacterial capsule biosynthesis	716:749	Here, we study the mechanisms involved in regulation of mycobacterial capsule biosynthesis using a high throughput screen for gene products involved in capsular α-glucan production.
27044743	5	7	theme	polysaccharides	1057:1071	arg1	overproduction					1030:1043	a strong overproduction	1021:1043	a strong overproduction of capsular polysaccharides, including α-glucan and arabinomannan, suggestive of a role for inorganic phosphate (Pi) metabolism in modulating capsular polysaccharide production	1021:1220	Utilizing this approach we identified a group of mutants that all carried mutations in the ATP-binding cassette phosphate transport locus pst These mutants collectively exhibited a strong overproduction of capsular polysaccharides, including α-glucan and arabinomannan, suggestive of a role for inorganic phosphate (Pi) metabolism in modulating capsular polysaccharide production.
27044743	6	8	theme	stringent	1349:1357	arg1	response					1359:1366	the stringent response	1345:1366	the stringent response	1345:1366	These findings were corroborated by the observation that growth under low Pi conditions as well as chemical activation of the stringent response induces capsule production in a number of mycobacterial species.
27044743	6	9	theme	low	1293:1295	arg1	conditions					1300:1309	low Pi conditions	1293:1309	low Pi conditions as well as chemical activation of the stringent response	1293:1366	These findings were corroborated by the observation that growth under low Pi conditions as well as chemical activation of the stringent response induces capsule production in a number of mycobacterial species.
27044743	7	10	theme	Mycobacterium	1508:1520	arg1	marinum					1522:1528	Mycobacterium marinum	1508:1528	Mycobacterium marinum	1508:1528	This induction is, in part, dependent on σ factor E. Finally, we show that Mycobacterium marinum, a model organism for M. tuberculosis, encounters Pi stress during infection, which shows the relevance of our findings in vivo.
27044743	7	10	theme	Mycobacterium	1508:1520	arg1	organism					1539:1546	a model organism	1531:1546	a model organism for M. tuberculosis	1531:1566	This induction is, in part, dependent on σ factor E. Finally, we show that Mycobacterium marinum, a model organism for M. tuberculosis, encounters Pi stress during infection, which shows the relevance of our findings in vivo.
27044743	5	11	theme	strong	1023:1028	arg1	overproduction					1030:1043	a strong overproduction	1021:1043	a strong overproduction of capsular polysaccharides, including α-glucan and arabinomannan, suggestive of a role for inorganic phosphate (Pi) metabolism in modulating capsular polysaccharide production	1021:1220	Utilizing this approach we identified a group of mutants that all carried mutations in the ATP-binding cassette phosphate transport locus pst These mutants collectively exhibited a strong overproduction of capsular polysaccharides, including α-glucan and arabinomannan, suggestive of a role for inorganic phosphate (Pi) metabolism in modulating capsular polysaccharide production.
27044743	5	12	theme	ATP-binding	933:943	arg1	pst					980:982	the ATP-binding cassette phosphate transport locus pst	929:982	the ATP-binding cassette phosphate transport locus pst	929:982	Utilizing this approach we identified a group of mutants that all carried mutations in the ATP-binding cassette phosphate transport locus pst These mutants collectively exhibited a strong overproduction of capsular polysaccharides, including α-glucan and arabinomannan, suggestive of a role for inorganic phosphate (Pi) metabolism in modulating capsular polysaccharide production.
27044743	1	13	theme	impermeable	159:169	arg1	envelope					176:183	an unusual and highly impermeable cell envelope	137:183	an unusual and highly impermeable cell envelope that is critically important for the successful colonization of the host	137:256	Mycobacterium tuberculosis is protected by an unusual and highly impermeable cell envelope that is critically important for the successful colonization of the host.
27044743	1	13	theme	impermeable	159:169	arg1	important					204:212	important	204:212	important	204:212	Mycobacterium tuberculosis is protected by an unusual and highly impermeable cell envelope that is critically important for the successful colonization of the host.
27044743	3	14	theme	polysaccharides	518:532	arg1	production					491:500	the production	487:500	the production of the capsular polysaccharides	487:532	Although the bioenzymatic steps involved in the production of the capsular polysaccharides are emerging, information regarding the ability of the bacterium to modulate the composition of the capsule is still unknown.
27044743	1	15	theme	Mycobacterium	94:106	arg1	tuberculosis					108:119	Mycobacterium tuberculosis	94:119	Mycobacterium tuberculosis	94:119	Mycobacterium tuberculosis is protected by an unusual and highly impermeable cell envelope that is critically important for the successful colonization of the host.
27044743	5	16	theme	locus	974:978	arg1	pst					980:982	the ATP-binding cassette phosphate transport locus pst	929:982	the ATP-binding cassette phosphate transport locus pst	929:982	Utilizing this approach we identified a group of mutants that all carried mutations in the ATP-binding cassette phosphate transport locus pst These mutants collectively exhibited a strong overproduction of capsular polysaccharides, including α-glucan and arabinomannan, suggestive of a role for inorganic phosphate (Pi) metabolism in modulating capsular polysaccharide production.
27044743	5	17	theme	cassette	945:952	arg1	pst					980:982	the ATP-binding cassette phosphate transport locus pst	929:982	the ATP-binding cassette phosphate transport locus pst	929:982	Utilizing this approach we identified a group of mutants that all carried mutations in the ATP-binding cassette phosphate transport locus pst These mutants collectively exhibited a strong overproduction of capsular polysaccharides, including α-glucan and arabinomannan, suggestive of a role for inorganic phosphate (Pi) metabolism in modulating capsular polysaccharide production.
27044743	2	18	theme	envelope	294:301	arg1	surface					273:279	The outermost surface	259:279	The outermost surface of this cell envelope	259:301	The outermost surface of this cell envelope is formed by capsular polysaccharides that play an important role in modulating the initial interactions once the bacillus enters the body.
27044743	0	19	theme	Phosphate	10:18	arg1	Limitation					20:29	Inorganic Phosphate Limitation	0:29	Inorganic Phosphate Limitation	0:29	Inorganic Phosphate Limitation Modulates Capsular Polysaccharide Composition in Mycobacteria.
27044743	6	20	theme	chemical	1322:1329	arg1	activation					1331:1340	chemical activation	1322:1340	low Pi conditions as well as chemical activation of the stringent response	1293:1366	These findings were corroborated by the observation that growth under low Pi conditions as well as chemical activation of the stringent response induces capsule production in a number of mycobacterial species.
27044743	7	21	theme	σ	1474:1474	arg1	factor					1476:1481	σ factor	1474:1481	σ factor	1474:1481	This induction is, in part, dependent on σ factor E. Finally, we show that Mycobacterium marinum, a model organism for M. tuberculosis, encounters Pi stress during infection, which shows the relevance of our findings in vivo.
27044743	5	22	theme	carried	908:914	arg1	mutations					916:924	all carried mutations	904:924	all carried mutations in the ATP-binding cassette phosphate transport locus pst	904:982	Utilizing this approach we identified a group of mutants that all carried mutations in the ATP-binding cassette phosphate transport locus pst These mutants collectively exhibited a strong overproduction of capsular polysaccharides, including α-glucan and arabinomannan, suggestive of a role for inorganic phosphate (Pi) metabolism in modulating capsular polysaccharide production.
27044743	0	23	theme	Inorganic	0:8	arg1	Limitation					20:29	Inorganic Phosphate Limitation	0:29	Inorganic Phosphate Limitation	0:29	Inorganic Phosphate Limitation Modulates Capsular Polysaccharide Composition in Mycobacteria.
27044743	7	24	theme	M.	1552:1553	arg1	tuberculosis					1555:1566	M. tuberculosis	1552:1566	M. tuberculosis	1552:1566	This induction is, in part, dependent on σ factor E. Finally, we show that Mycobacterium marinum, a model organism for M. tuberculosis, encounters Pi stress during infection, which shows the relevance of our findings in vivo.
27044743	4	25	theme	throughput	764:773	arg1	screen					775:780	a high throughput screen	757:780	a high throughput screen for gene products involved in capsular α-glucan production	757:839	Here, we study the mechanisms involved in regulation of mycobacterial capsule biosynthesis using a high throughput screen for gene products involved in capsular α-glucan production.
27044743	3	26	theme	bioenzymatic	456:467	arg1	steps					469:473	the bioenzymatic steps	452:473	the bioenzymatic steps involved in the production of the capsular polysaccharides	452:532	Although the bioenzymatic steps involved in the production of the capsular polysaccharides are emerging, information regarding the ability of the bacterium to modulate the composition of the capsule is still unknown.
27044743	6	27	theme	species	1424:1430	arg1	number					1400:1405	a number	1398:1405	a number of mycobacterial species	1398:1430	These findings were corroborated by the observation that growth under low Pi conditions as well as chemical activation of the stringent response induces capsule production in a number of mycobacterial species.
27044743	3	28	theme	bacterium	589:597	arg1	ability					574:580	the ability	570:580	the ability of the bacterium to modulate the composition of the capsule	570:640	Although the bioenzymatic steps involved in the production of the capsular polysaccharides are emerging, information regarding the ability of the bacterium to modulate the composition of the capsule is still unknown.
27044743	1	29	theme	successful	222:231	arg1	colonization					233:244	the successful colonization	218:244	the successful colonization of the host	218:256	Mycobacterium tuberculosis is protected by an unusual and highly impermeable cell envelope that is critically important for the successful colonization of the host.
27044743	2	30	dep	interactions	395:406	arg1	enters					426:431	enters	426:431	enters the body	426:440	The outermost surface of this cell envelope is formed by capsular polysaccharides that play an important role in modulating the initial interactions once the bacillus enters the body.
27044743	2	31	theme	capsular	316:323	arg1	polysaccharides					325:339	capsular polysaccharides	316:339	capsular polysaccharides that play an important role in modulating the initial interactions once the bacillus enters the body	316:440	The outermost surface of this cell envelope is formed by capsular polysaccharides that play an important role in modulating the initial interactions once the bacillus enters the body.
27044743	7	32	from	part	1455:1458	arg1	dependent					1461:1469	dependent	1461:1469	dependent	1461:1469	This induction is, in part, dependent on σ factor E. Finally, we show that Mycobacterium marinum, a model organism for M. tuberculosis, encounters Pi stress during infection, which shows the relevance of our findings in vivo.
27044743	7	33	dep	dependent	1461:1469	arg1	E.					1483:1484	E.	1483:1484	E.	1483:1484	This induction is, in part, dependent on σ factor E. Finally, we show that Mycobacterium marinum, a model organism for M. tuberculosis, encounters Pi stress during infection, which shows the relevance of our findings in vivo.
27044743	0	34	theme	Polysaccharide	50:63	arg1	Composition					65:75	Capsular Polysaccharide Composition	41:75	Capsular Polysaccharide Composition	41:75	Inorganic Phosphate Limitation Modulates Capsular Polysaccharide Composition in Mycobacteria.
27044743	5	35	theme	transport	964:972	arg1	pst					980:982	the ATP-binding cassette phosphate transport locus pst	929:982	the ATP-binding cassette phosphate transport locus pst	929:982	Utilizing this approach we identified a group of mutants that all carried mutations in the ATP-binding cassette phosphate transport locus pst These mutants collectively exhibited a strong overproduction of capsular polysaccharides, including α-glucan and arabinomannan, suggestive of a role for inorganic phosphate (Pi) metabolism in modulating capsular polysaccharide production.
27044743	5	36	theme	inorganic	1137:1145	arg1	Pi					1158:1159	Pi	1158:1159	Pi	1158:1159	Utilizing this approach we identified a group of mutants that all carried mutations in the ATP-binding cassette phosphate transport locus pst These mutants collectively exhibited a strong overproduction of capsular polysaccharides, including α-glucan and arabinomannan, suggestive of a role for inorganic phosphate (Pi) metabolism in modulating capsular polysaccharide production.
27044743	5	36	theme	inorganic	1137:1145	arg1	phosphate					1147:1155	inorganic phosphate	1137:1155	inorganic phosphate (Pi) metabolism	1137:1171	Utilizing this approach we identified a group of mutants that all carried mutations in the ATP-binding cassette phosphate transport locus pst These mutants collectively exhibited a strong overproduction of capsular polysaccharides, including α-glucan and arabinomannan, suggestive of a role for inorganic phosphate (Pi) metabolism in modulating capsular polysaccharide production.
27044743	2	37	theme	outermost	263:271	arg1	surface					273:279	The outermost surface	259:279	The outermost surface of this cell envelope	259:301	The outermost surface of this cell envelope is formed by capsular polysaccharides that play an important role in modulating the initial interactions once the bacillus enters the body.
27044743	0	38	theme	Capsular	41:48	arg1	Composition					65:75	Capsular Polysaccharide Composition	41:75	Capsular Polysaccharide Composition	41:75	Inorganic Phosphate Limitation Modulates Capsular Polysaccharide Composition in Mycobacteria.
27044743	5	39	theme	phosphate	1147:1155	arg1	metabolism					1162:1171	inorganic phosphate (Pi) metabolism	1137:1171	inorganic phosphate (Pi) metabolism	1137:1171	Utilizing this approach we identified a group of mutants that all carried mutations in the ATP-binding cassette phosphate transport locus pst These mutants collectively exhibited a strong overproduction of capsular polysaccharides, including α-glucan and arabinomannan, suggestive of a role for inorganic phosphate (Pi) metabolism in modulating capsular polysaccharide production.
27044743	3	40	theme	capsule	634:640	arg1	composition					615:625	the composition	611:625	the composition of the capsule	611:640	Although the bioenzymatic steps involved in the production of the capsular polysaccharides are emerging, information regarding the ability of the bacterium to modulate the composition of the capsule is still unknown.
27044743	5	41	theme	mutants	891:897	arg1	mutants					891:897	mutants	891:897	mutants that all carried mutations in the ATP-binding cassette phosphate transport locus pst These mutants collectively exhibited a strong overproduction of capsular polysaccharides, including α-glucan and arabinomannan, suggestive of a role for inorganic phosphate (Pi) metabolism in modulating capsular polysaccharide production	891:1220	Utilizing this approach we identified a group of mutants that all carried mutations in the ATP-binding cassette phosphate transport locus pst These mutants collectively exhibited a strong overproduction of capsular polysaccharides, including α-glucan and arabinomannan, suggestive of a role for inorganic phosphate (Pi) metabolism in modulating capsular polysaccharide production.
27044743	5	41	theme	mutants	891:897	arg1	group					882:886	a group	880:886	a group of mutants that all carried mutations in the ATP-binding cassette phosphate transport locus pst These mutants collectively exhibited a strong overproduction of capsular polysaccharides, including α-glucan and arabinomannan, suggestive of a role for inorganic phosphate (Pi) metabolism in modulating capsular polysaccharide production	880:1220	Utilizing this approach we identified a group of mutants that all carried mutations in the ATP-binding cassette phosphate transport locus pst These mutants collectively exhibited a strong overproduction of capsular polysaccharides, including α-glucan and arabinomannan, suggestive of a role for inorganic phosphate (Pi) metabolism in modulating capsular polysaccharide production.
27044743	7	42	theme	Pi	1580:1581	arg1	stress					1583:1588	Pi stress	1580:1588	Pi stress	1580:1588	This induction is, in part, dependent on σ factor E. Finally, we show that Mycobacterium marinum, a model organism for M. tuberculosis, encounters Pi stress during infection, which shows the relevance of our findings in vivo.
27044743	1	43	theme	unusual	140:146	arg1	envelope					176:183	an unusual and highly impermeable cell envelope	137:183	an unusual and highly impermeable cell envelope that is critically important for the successful colonization of the host	137:256	Mycobacterium tuberculosis is protected by an unusual and highly impermeable cell envelope that is critically important for the successful colonization of the host.
27044743	1	43	theme	unusual	140:146	arg1	important					204:212	important	204:212	important	204:212	Mycobacterium tuberculosis is protected by an unusual and highly impermeable cell envelope that is critically important for the successful colonization of the host.
27044743	7	44	theme	findings	1641:1648	arg1	relevance					1624:1632	the relevance	1620:1632	the relevance of our findings	1620:1648	This induction is, in part, dependent on σ factor E. Finally, we show that Mycobacterium marinum, a model organism for M. tuberculosis, encounters Pi stress during infection, which shows the relevance of our findings in vivo.
27044743	5	45	theme	suggestive	1112:1121	arg1	α-glucan					1084:1091	α-glucan	1084:1091	α-glucan	1084:1091	Utilizing this approach we identified a group of mutants that all carried mutations in the ATP-binding cassette phosphate transport locus pst These mutants collectively exhibited a strong overproduction of capsular polysaccharides, including α-glucan and arabinomannan, suggestive of a role for inorganic phosphate (Pi) metabolism in modulating capsular polysaccharide production.
27044743	6	46	theme	Pi	1297:1298	arg1	conditions					1300:1309	low Pi conditions	1293:1309	low Pi conditions as well as chemical activation of the stringent response	1293:1366	These findings were corroborated by the observation that growth under low Pi conditions as well as chemical activation of the stringent response induces capsule production in a number of mycobacterial species.
27044743	4	47	theme	biosynthesis	738:749	arg1	regulation					702:711	regulation	702:711	regulation of mycobacterial capsule biosynthesis	702:749	Here, we study the mechanisms involved in regulation of mycobacterial capsule biosynthesis using a high throughput screen for gene products involved in capsular α-glucan production.
27044743	7	48	from	dependent	1461:1469	arg1	part					1455:1458	part	1455:1458	part	1455:1458	This induction is, in part, dependent on σ factor E. Finally, we show that Mycobacterium marinum, a model organism for M. tuberculosis, encounters Pi stress during infection, which shows the relevance of our findings in vivo.
27044743	6	49	theme	response	1359:1366	arg1	activation					1331:1340	chemical activation	1322:1340	low Pi conditions as well as chemical activation of the stringent response	1293:1366	These findings were corroborated by the observation that growth under low Pi conditions as well as chemical activation of the stringent response induces capsule production in a number of mycobacterial species.
27044743	6	49	theme	response	1359:1366	arg1	conditions					1300:1309	low Pi conditions	1293:1309	low Pi conditions as well as chemical activation of the stringent response	1293:1366	These findings were corroborated by the observation that growth under low Pi conditions as well as chemical activation of the stringent response induces capsule production in a number of mycobacterial species.
27044743	5	50	theme	capsular	1048:1055	arg1	arabinomannan					1097:1109	arabinomannan	1097:1109	arabinomannan	1097:1109	Utilizing this approach we identified a group of mutants that all carried mutations in the ATP-binding cassette phosphate transport locus pst These mutants collectively exhibited a strong overproduction of capsular polysaccharides, including α-glucan and arabinomannan, suggestive of a role for inorganic phosphate (Pi) metabolism in modulating capsular polysaccharide production.
27044743	5	50	theme	capsular	1048:1055	arg1	polysaccharides					1057:1071	capsular polysaccharides	1048:1071	capsular polysaccharides	1048:1071	Utilizing this approach we identified a group of mutants that all carried mutations in the ATP-binding cassette phosphate transport locus pst These mutants collectively exhibited a strong overproduction of capsular polysaccharides, including α-glucan and arabinomannan, suggestive of a role for inorganic phosphate (Pi) metabolism in modulating capsular polysaccharide production.
27044743	5	50	theme	capsular	1048:1055	arg1	α-glucan					1084:1091	α-glucan	1084:1091	α-glucan	1084:1091	Utilizing this approach we identified a group of mutants that all carried mutations in the ATP-binding cassette phosphate transport locus pst These mutants collectively exhibited a strong overproduction of capsular polysaccharides, including α-glucan and arabinomannan, suggestive of a role for inorganic phosphate (Pi) metabolism in modulating capsular polysaccharide production.
27044743	2	51	theme	cell	289:292	arg1	envelope					294:301	this cell envelope	284:301	this cell envelope	284:301	The outermost surface of this cell envelope is formed by capsular polysaccharides that play an important role in modulating the initial interactions once the bacillus enters the body.
27044743	2	52	theme	important	354:362	arg1	role					364:367	an important role	351:367	an important role	351:367	The outermost surface of this cell envelope is formed by capsular polysaccharides that play an important role in modulating the initial interactions once the bacillus enters the body.
27044743	4	53	theme	gene	786:789	arg1	products					791:798	gene products	786:798	gene products involved in capsular α-glucan production	786:839	Here, we study the mechanisms involved in regulation of mycobacterial capsule biosynthesis using a high throughput screen for gene products involved in capsular α-glucan production.
27044743	1	54	theme	host	253:256	arg1	colonization					233:244	the successful colonization	218:244	the successful colonization of the host	218:256	Mycobacterium tuberculosis is protected by an unusual and highly impermeable cell envelope that is critically important for the successful colonization of the host.
27044743	4	55	theme	capsular	812:819	arg1	production					830:839	capsular α-glucan production	812:839	capsular α-glucan production	812:839	Here, we study the mechanisms involved in regulation of mycobacterial capsule biosynthesis using a high throughput screen for gene products involved in capsular α-glucan production.
27044743	5	56	theme	phosphate	954:962	arg1	pst					980:982	the ATP-binding cassette phosphate transport locus pst	929:982	the ATP-binding cassette phosphate transport locus pst	929:982	Utilizing this approach we identified a group of mutants that all carried mutations in the ATP-binding cassette phosphate transport locus pst These mutants collectively exhibited a strong overproduction of capsular polysaccharides, including α-glucan and arabinomannan, suggestive of a role for inorganic phosphate (Pi) metabolism in modulating capsular polysaccharide production.
27044743	4	57	theme	α-glucan	821:828	arg1	production					830:839	capsular α-glucan production	812:839	capsular α-glucan production	812:839	Here, we study the mechanisms involved in regulation of mycobacterial capsule biosynthesis using a high throughput screen for gene products involved in capsular α-glucan production.
27044743	3	58	theme	capsular	509:516	arg1	polysaccharides					518:532	the capsular polysaccharides	505:532	the capsular polysaccharides	505:532	Although the bioenzymatic steps involved in the production of the capsular polysaccharides are emerging, information regarding the ability of the bacterium to modulate the composition of the capsule is still unknown.
27044743	4	59	theme	high	759:762	arg1	screen					775:780	a high throughput screen	757:780	a high throughput screen for gene products involved in capsular α-glucan production	757:839	Here, we study the mechanisms involved in regulation of mycobacterial capsule biosynthesis using a high throughput screen for gene products involved in capsular α-glucan production.
27044743	5	60	theme	role	1128:1131	arg1	suggestive					1112:1121	suggestive	1112:1121	suggestive	1112:1121	Utilizing this approach we identified a group of mutants that all carried mutations in the ATP-binding cassette phosphate transport locus pst These mutants collectively exhibited a strong overproduction of capsular polysaccharides, including α-glucan and arabinomannan, suggestive of a role for inorganic phosphate (Pi) metabolism in modulating capsular polysaccharide production.
27044743	5	61	from	mutations	916:924	arg1	pst					980:982	the ATP-binding cassette phosphate transport locus pst	929:982	the ATP-binding cassette phosphate transport locus pst	929:982	Utilizing this approach we identified a group of mutants that all carried mutations in the ATP-binding cassette phosphate transport locus pst These mutants collectively exhibited a strong overproduction of capsular polysaccharides, including α-glucan and arabinomannan, suggestive of a role for inorganic phosphate (Pi) metabolism in modulating capsular polysaccharide production.
27044743	1	62	theme	cell	171:174	arg1	envelope					176:183	an unusual and highly impermeable cell envelope	137:183	an unusual and highly impermeable cell envelope that is critically important for the successful colonization of the host	137:256	Mycobacterium tuberculosis is protected by an unusual and highly impermeable cell envelope that is critically important for the successful colonization of the host.
27044743	1	62	theme	cell	171:174	arg1	important					204:212	important	204:212	important	204:212	Mycobacterium tuberculosis is protected by an unusual and highly impermeable cell envelope that is critically important for the successful colonization of the host.
27044743	7	63	theme	model	1533:1537	arg1	marinum					1522:1528	Mycobacterium marinum	1508:1528	Mycobacterium marinum	1508:1528	This induction is, in part, dependent on σ factor E. Finally, we show that Mycobacterium marinum, a model organism for M. tuberculosis, encounters Pi stress during infection, which shows the relevance of our findings in vivo.
27044743	7	63	theme	model	1533:1537	arg1	organism					1539:1546	a model organism	1531:1546	a model organism for M. tuberculosis	1531:1566	This induction is, in part, dependent on σ factor E. Finally, we show that Mycobacterium marinum, a model organism for M. tuberculosis, encounters Pi stress during infection, which shows the relevance of our findings in vivo.
27044743	2	64	theme	initial	387:393	arg1	interactions					395:406	the initial interactions	383:406	the initial interactions once the bacillus enters the body	383:440	The outermost surface of this cell envelope is formed by capsular polysaccharides that play an important role in modulating the initial interactions once the bacillus enters the body.
24693976	0	0	theme	Lactobacillus	86:98	arg1	strains					107:113	Lactobacillus brevis strains	86:113	Lactobacillus brevis strains isolated from fermented Japanese pickles	86:154	Cellular fatty acid composition and exopolysaccharide contribute to bile tolerance in Lactobacillus brevis strains isolated from fermented Japanese pickles.
24693976	7	1	from	culture	1183:1189	arg1	MRS					1194:1196	MRS	1194:1196	MRS without sodium acetate	1194:1219	Meanwhile, culture in MRS without sodium acetate reduced EPS-b production in aggregating strains.
24693976	2	2	theme	exopolysaccharide	378:394	arg1	production					404:413	cell-bound exopolysaccharide (EPS-b) production	367:413	cell-bound exopolysaccharide (EPS-b) production	367:413	We examined this property in 56 Lactobacillus brevis strains isolated from Japanese pickles and also evaluated cellular fatty acid composition and cell-bound exopolysaccharide (EPS-b) production.
24693976	6	3	theme	bile	1166:1169	arg1	presence					1154:1161	the presence	1150:1161	the presence of bile	1150:1169	In MRS without sodium acetate, only the 3 most tolerant nonaggregating strains increased their relative C18:1 content in the presence of bile.
24693976	8	4	theme	cellular	1300:1307	arg1	composition					1320:1330	cellular fatty acid composition	1300:1330	cellular fatty acid composition	1300:1330	In conclusion, both EPS-b and cellular fatty acid composition play important roles in bile tolerance of pickle-derived L. brevis.
24693976	4	5	theme	nonaggregating	660:673	arg1	strains					675:681	nonaggregating strains	660:681	nonaggregating strains	660:681	Aggregating strains showed significantly higher bile tolerance than nonaggregating strains in MRS medium, but there was no significant difference in the modified MRS media.
24693976	0	6	theme	brevis	100:105	arg1	strains					107:113	Lactobacillus brevis strains	86:113	Lactobacillus brevis strains isolated from fermented Japanese pickles	86:154	Cellular fatty acid composition and exopolysaccharide contribute to bile tolerance in Lactobacillus brevis strains isolated from fermented Japanese pickles.
24693976	4	7	theme	MRS	754:756	arg1	media					758:762	the modified MRS media	741:762	the modified MRS media	741:762	Aggregating strains showed significantly higher bile tolerance than nonaggregating strains in MRS medium, but there was no significant difference in the modified MRS media.
24693976	5	8	theme	aggregating	835:845	arg1	strains					866:872	the 3 most tolerant aggregating and nonaggregating strains	815:872	the 3 most tolerant aggregating and nonaggregating strains	815:872	The relative octadecenoic acid (C18:1) content of the 3 most tolerant aggregating and nonaggregating strains was significantly higher when bile was added to MRS. In MRS without Tween 80, the relative C18:1 content was only marginally affected by addition of bile.
24693976	6	9	theme	nonaggregating	1085:1098	arg1	strains					1100:1106	only the 3 most tolerant nonaggregating strains	1060:1106	only the 3 most tolerant nonaggregating strains	1060:1106	In MRS without sodium acetate, only the 3 most tolerant nonaggregating strains increased their relative C18:1 content in the presence of bile.
24693976	6	10	theme	sodium	1044:1049	arg1	acetate					1051:1057	sodium acetate	1044:1057	sodium acetate	1044:1057	In MRS without sodium acetate, only the 3 most tolerant nonaggregating strains increased their relative C18:1 content in the presence of bile.
24693976	2	11	theme	Japanese	295:302	arg1	pickles					304:310	Japanese pickles	295:310	Japanese pickles	295:310	We examined this property in 56 Lactobacillus brevis strains isolated from Japanese pickles and also evaluated cellular fatty acid composition and cell-bound exopolysaccharide (EPS-b) production.
24693976	1	12	theme	fundamental	177:187	arg1	tolerance					162:170	Bile tolerance	157:170	Bile tolerance	157:170	Bile tolerance is a fundamental ability of probiotic bacteria.
24693976	1	12	theme	fundamental	177:187	arg1	ability					189:195	a fundamental ability	175:195	a fundamental ability of probiotic bacteria	175:217	Bile tolerance is a fundamental ability of probiotic bacteria.
24693976	8	13	link	pickle-derived	1374:1387	arg1	brevis					1392:1397	pickle-derived L. brevis	1374:1397	pickle-derived L. brevis	1374:1397	In conclusion, both EPS-b and cellular fatty acid composition play important roles in bile tolerance of pickle-derived L. brevis.
24693976	8	14	theme	fatty	1309:1313	arg1	composition					1320:1330	cellular fatty acid composition	1300:1330	cellular fatty acid composition	1300:1330	In conclusion, both EPS-b and cellular fatty acid composition play important roles in bile tolerance of pickle-derived L. brevis.
24693976	4	15	theme	higher	633:638	arg1	tolerance					645:653	significantly higher bile tolerance	619:653	significantly higher bile tolerance than nonaggregating strains in MRS medium	619:695	Aggregating strains showed significantly higher bile tolerance than nonaggregating strains in MRS medium, but there was no significant difference in the modified MRS media.
24693976	3	16	theme	bile	420:423	arg1	lower					470:474	lower	470:474	lower	470:474	The bile tolerance of these strains was significantly lower in modified de Man - Rogosa - Sharpe (MRS) medium (without Tween 80 or sodium acetate) than in standard MRS medium.
24693976	3	16	theme	bile	420:423	arg1	tolerance					425:433	The bile tolerance	416:433	The bile tolerance of these strains	416:450	The bile tolerance of these strains was significantly lower in modified de Man - Rogosa - Sharpe (MRS) medium (without Tween 80 or sodium acetate) than in standard MRS medium.
24693976	8	17	theme	acid	1315:1318	arg1	composition					1320:1330	cellular fatty acid composition	1300:1330	cellular fatty acid composition	1300:1330	In conclusion, both EPS-b and cellular fatty acid composition play important roles in bile tolerance of pickle-derived L. brevis.
24693976	0	18	theme	fatty	9:13	arg1	composition					20:30	Cellular fatty acid composition	0:30	Cellular fatty acid composition	0:30	Cellular fatty acid composition and exopolysaccharide contribute to bile tolerance in Lactobacillus brevis strains isolated from fermented Japanese pickles.
24693976	6	19	theme	relative	1124:1131	arg1	content					1139:1145	their relative C18:1 content	1118:1145	their relative C18:1 content	1118:1145	In MRS without sodium acetate, only the 3 most tolerant nonaggregating strains increased their relative C18:1 content in the presence of bile.
24693976	2	20	theme	fatty	340:344	arg1	composition					351:361	cellular fatty acid composition	331:361	cellular fatty acid composition	331:361	We examined this property in 56 Lactobacillus brevis strains isolated from Japanese pickles and also evaluated cellular fatty acid composition and cell-bound exopolysaccharide (EPS-b) production.
24693976	7	21	theme	EPS-b	1229:1233	arg1	production					1235:1244	EPS-b production	1229:1244	EPS-b production	1229:1244	Meanwhile, culture in MRS without sodium acetate reduced EPS-b production in aggregating strains.
24693976	5	22	theme	nonaggregating	851:864	arg1	strains					866:872	the 3 most tolerant aggregating and nonaggregating strains	815:872	the 3 most tolerant aggregating and nonaggregating strains	815:872	The relative octadecenoic acid (C18:1) content of the 3 most tolerant aggregating and nonaggregating strains was significantly higher when bile was added to MRS. In MRS without Tween 80, the relative C18:1 content was only marginally affected by addition of bile.
24693976	0	23	theme	Cellular	0:7	arg1	composition					20:30	Cellular fatty acid composition	0:30	Cellular fatty acid composition	0:30	Cellular fatty acid composition and exopolysaccharide contribute to bile tolerance in Lactobacillus brevis strains isolated from fermented Japanese pickles.
24693976	2	24	theme	cellular	331:338	arg1	composition					351:361	cellular fatty acid composition	331:361	cellular fatty acid composition	331:361	We examined this property in 56 Lactobacillus brevis strains isolated from Japanese pickles and also evaluated cellular fatty acid composition and cell-bound exopolysaccharide (EPS-b) production.
24693976	4	25	from	tolerance	645:653	arg1	medium					690:695	MRS medium	686:695	MRS medium	686:695	Aggregating strains showed significantly higher bile tolerance than nonaggregating strains in MRS medium, but there was no significant difference in the modified MRS media.
24693976	1	26	theme	probiotic	200:208	arg1	bacteria					210:217	probiotic bacteria	200:217	probiotic bacteria	200:217	Bile tolerance is a fundamental ability of probiotic bacteria.
24693976	0	27	theme	Japanese	139:146	arg1	pickles					148:154	fermented Japanese pickles	129:154	fermented Japanese pickles	129:154	Cellular fatty acid composition and exopolysaccharide contribute to bile tolerance in Lactobacillus brevis strains isolated from fermented Japanese pickles.
24693976	4	28	from	difference	727:736	arg1	media					758:762	the modified MRS media	741:762	the modified MRS media	741:762	Aggregating strains showed significantly higher bile tolerance than nonaggregating strains in MRS medium, but there was no significant difference in the modified MRS media.
24693976	3	29	theme	sodium	547:552	arg1	acetate					554:560	sodium acetate	547:560	sodium acetate	547:560	The bile tolerance of these strains was significantly lower in modified de Man - Rogosa - Sharpe (MRS) medium (without Tween 80 or sodium acetate) than in standard MRS medium.
24693976	8	30	theme	bile	1356:1359	arg1	tolerance					1361:1369	bile tolerance	1356:1369	bile tolerance of pickle-derived L. brevis	1356:1397	In conclusion, both EPS-b and cellular fatty acid composition play important roles in bile tolerance of pickle-derived L. brevis.
24693976	1	31	theme	bacteria	210:217	arg1	tolerance					162:170	Bile tolerance	157:170	Bile tolerance	157:170	Bile tolerance is a fundamental ability of probiotic bacteria.
24693976	1	31	theme	bacteria	210:217	arg1	ability					189:195	a fundamental ability	175:195	a fundamental ability of probiotic bacteria	175:217	Bile tolerance is a fundamental ability of probiotic bacteria.
24693976	0	32	theme	acid	15:18	arg1	composition					20:30	Cellular fatty acid composition	0:30	Cellular fatty acid composition	0:30	Cellular fatty acid composition and exopolysaccharide contribute to bile tolerance in Lactobacillus brevis strains isolated from fermented Japanese pickles.
24693976	0	33	theme	fermented	129:137	arg1	pickles					148:154	fermented Japanese pickles	129:154	fermented Japanese pickles	129:154	Cellular fatty acid composition and exopolysaccharide contribute to bile tolerance in Lactobacillus brevis strains isolated from fermented Japanese pickles.
24693976	8	34	theme	important	1337:1345	arg1	roles					1347:1351	important roles	1337:1351	important roles	1337:1351	In conclusion, both EPS-b and cellular fatty acid composition play important roles in bile tolerance of pickle-derived L. brevis.
24693976	7	35	theme	sodium	1206:1211	arg1	acetate					1213:1219	sodium acetate	1206:1219	sodium acetate	1206:1219	Meanwhile, culture in MRS without sodium acetate reduced EPS-b production in aggregating strains.
24693976	5	36	theme	relative	769:776	arg1	higher					892:897	higher	892:897	higher	892:897	The relative octadecenoic acid (C18:1) content of the 3 most tolerant aggregating and nonaggregating strains was significantly higher when bile was added to MRS. In MRS without Tween 80, the relative C18:1 content was only marginally affected by addition of bile.
24693976	5	36	theme	relative	769:776	arg1	content					804:810	The relative octadecenoic acid (C18:1) content	765:810	The relative octadecenoic acid (C18:1) content of the 3 most tolerant aggregating and nonaggregating strains	765:872	The relative octadecenoic acid (C18:1) content of the 3 most tolerant aggregating and nonaggregating strains was significantly higher when bile was added to MRS. In MRS without Tween 80, the relative C18:1 content was only marginally affected by addition of bile.
24693976	6	37	theme	tolerant	1076:1083	arg1	strains					1100:1106	only the 3 most tolerant nonaggregating strains	1060:1106	only the 3 most tolerant nonaggregating strains	1060:1106	In MRS without sodium acetate, only the 3 most tolerant nonaggregating strains increased their relative C18:1 content in the presence of bile.
24693976	7	38	theme	aggregating	1249:1259	arg1	strains					1261:1267	aggregating strains	1249:1267	aggregating strains	1249:1267	Meanwhile, culture in MRS without sodium acetate reduced EPS-b production in aggregating strains.
24693976	3	39	theme	MRS	580:582	arg1	medium					584:589	standard MRS medium	571:589	standard MRS medium	571:589	The bile tolerance of these strains was significantly lower in modified de Man - Rogosa - Sharpe (MRS) medium (without Tween 80 or sodium acetate) than in standard MRS medium.
24693976	5	40	theme	strains	866:872	arg1	higher					892:897	higher	892:897	higher	892:897	The relative octadecenoic acid (C18:1) content of the 3 most tolerant aggregating and nonaggregating strains was significantly higher when bile was added to MRS. In MRS without Tween 80, the relative C18:1 content was only marginally affected by addition of bile.
24693976	5	40	theme	strains	866:872	arg1	content					804:810	The relative octadecenoic acid (C18:1) content	765:810	The relative octadecenoic acid (C18:1) content of the 3 most tolerant aggregating and nonaggregating strains	765:872	The relative octadecenoic acid (C18:1) content of the 3 most tolerant aggregating and nonaggregating strains was significantly higher when bile was added to MRS. In MRS without Tween 80, the relative C18:1 content was only marginally affected by addition of bile.
24693976	3	41	theme	modified	479:486	arg1	medium					519:524	modified de Man - Rogosa - Sharpe (MRS) medium	479:524	modified de Man - Rogosa - Sharpe (MRS) medium (without Tween 80 or sodium acetate)	479:561	The bile tolerance of these strains was significantly lower in modified de Man - Rogosa - Sharpe (MRS) medium (without Tween 80 or sodium acetate) than in standard MRS medium.
24693976	3	42	theme	strains	444:450	arg1	lower					470:474	lower	470:474	lower	470:474	The bile tolerance of these strains was significantly lower in modified de Man - Rogosa - Sharpe (MRS) medium (without Tween 80 or sodium acetate) than in standard MRS medium.
24693976	3	42	theme	strains	444:450	arg1	tolerance					425:433	The bile tolerance	416:433	The bile tolerance of these strains	416:450	The bile tolerance of these strains was significantly lower in modified de Man - Rogosa - Sharpe (MRS) medium (without Tween 80 or sodium acetate) than in standard MRS medium.
24693976	5	43	theme	octadecenoic	778:789	arg1	acid					791:794	octadecenoic acid	778:794	The relative octadecenoic acid (C18:1) content of the 3 most tolerant aggregating and nonaggregating strains	765:872	The relative octadecenoic acid (C18:1) content of the 3 most tolerant aggregating and nonaggregating strains was significantly higher when bile was added to MRS. In MRS without Tween 80, the relative C18:1 content was only marginally affected by addition of bile.
24693976	5	43	theme	octadecenoic	778:789	arg1	C18:1					797:801	C18:1	797:801	C18:1	797:801	The relative octadecenoic acid (C18:1) content of the 3 most tolerant aggregating and nonaggregating strains was significantly higher when bile was added to MRS. In MRS without Tween 80, the relative C18:1 content was only marginally affected by addition of bile.
24693976	0	44	from	tolerance	73:81	arg1	strains					107:113	Lactobacillus brevis strains	86:113	Lactobacillus brevis strains isolated from fermented Japanese pickles	86:154	Cellular fatty acid composition and exopolysaccharide contribute to bile tolerance in Lactobacillus brevis strains isolated from fermented Japanese pickles.
24693976	3	45	theme	de	488:489	arg1	Man					491:493	de Man	488:493	de Man	488:493	The bile tolerance of these strains was significantly lower in modified de Man - Rogosa - Sharpe (MRS) medium (without Tween 80 or sodium acetate) than in standard MRS medium.
24693976	5	46	theme	acid	791:794	arg1	higher					892:897	higher	892:897	higher	892:897	The relative octadecenoic acid (C18:1) content of the 3 most tolerant aggregating and nonaggregating strains was significantly higher when bile was added to MRS. In MRS without Tween 80, the relative C18:1 content was only marginally affected by addition of bile.
24693976	5	46	theme	acid	791:794	arg1	content					804:810	The relative octadecenoic acid (C18:1) content	765:810	The relative octadecenoic acid (C18:1) content of the 3 most tolerant aggregating and nonaggregating strains	765:872	The relative octadecenoic acid (C18:1) content of the 3 most tolerant aggregating and nonaggregating strains was significantly higher when bile was added to MRS. In MRS without Tween 80, the relative C18:1 content was only marginally affected by addition of bile.
24693976	2	47	theme	cell-bound	367:376	arg1	production					404:413	cell-bound exopolysaccharide (EPS-b) production	367:413	cell-bound exopolysaccharide (EPS-b) production	367:413	We examined this property in 56 Lactobacillus brevis strains isolated from Japanese pickles and also evaluated cellular fatty acid composition and cell-bound exopolysaccharide (EPS-b) production.
24693976	3	48	theme	standard	571:578	arg1	medium					584:589	standard MRS medium	571:589	standard MRS medium	571:589	The bile tolerance of these strains was significantly lower in modified de Man - Rogosa - Sharpe (MRS) medium (without Tween 80 or sodium acetate) than in standard MRS medium.
24693976	4	49	theme	significant	715:725	arg1	difference					727:736	no significant difference	712:736	no significant difference in the modified MRS media	712:762	Aggregating strains showed significantly higher bile tolerance than nonaggregating strains in MRS medium, but there was no significant difference in the modified MRS media.
24693976	8	50	theme	brevis	1392:1397	arg1	tolerance					1361:1369	bile tolerance	1356:1369	bile tolerance of pickle-derived L. brevis	1356:1397	In conclusion, both EPS-b and cellular fatty acid composition play important roles in bile tolerance of pickle-derived L. brevis.
24693976	8	51	theme	L.	1389:1390	arg1	brevis					1392:1397	pickle-derived L. brevis	1374:1397	pickle-derived L. brevis	1374:1397	In conclusion, both EPS-b and cellular fatty acid composition play important roles in bile tolerance of pickle-derived L. brevis.
24693976	5	52	theme	relative	956:963	arg1	content					971:977	the relative C18:1 content	952:977	the relative C18:1 content	952:977	The relative octadecenoic acid (C18:1) content of the 3 most tolerant aggregating and nonaggregating strains was significantly higher when bile was added to MRS. In MRS without Tween 80, the relative C18:1 content was only marginally affected by addition of bile.
24693976	3	53	theme	Rogosa	497:502	arg1	medium					519:524	modified de Man - Rogosa - Sharpe (MRS) medium	479:524	modified de Man - Rogosa - Sharpe (MRS) medium (without Tween 80 or sodium acetate)	479:561	The bile tolerance of these strains was significantly lower in modified de Man - Rogosa - Sharpe (MRS) medium (without Tween 80 or sodium acetate) than in standard MRS medium.
24693976	4	54	theme	modified	745:752	arg1	media					758:762	the modified MRS media	741:762	the modified MRS media	741:762	Aggregating strains showed significantly higher bile tolerance than nonaggregating strains in MRS medium, but there was no significant difference in the modified MRS media.
24693976	4	55	theme	Aggregating	592:602	arg1	strains					604:610	Aggregating strains	592:610	Aggregating strains	592:610	Aggregating strains showed significantly higher bile tolerance than nonaggregating strains in MRS medium, but there was no significant difference in the modified MRS media.
24693976	6	56	theme	C18:1	1133:1137	arg1	content					1139:1145	their relative C18:1 content	1118:1145	their relative C18:1 content	1118:1145	In MRS without sodium acetate, only the 3 most tolerant nonaggregating strains increased their relative C18:1 content in the presence of bile.
24693976	5	57	theme	bile	1023:1026	arg1	addition					1011:1018	addition	1011:1018	addition of bile	1011:1026	The relative octadecenoic acid (C18:1) content of the 3 most tolerant aggregating and nonaggregating strains was significantly higher when bile was added to MRS. In MRS without Tween 80, the relative C18:1 content was only marginally affected by addition of bile.
24693976	5	58	theme	C18:1	965:969	arg1	content					971:977	the relative C18:1 content	952:977	the relative C18:1 content	952:977	The relative octadecenoic acid (C18:1) content of the 3 most tolerant aggregating and nonaggregating strains was significantly higher when bile was added to MRS. In MRS without Tween 80, the relative C18:1 content was only marginally affected by addition of bile.
24693976	2	59	theme	acid	346:349	arg1	composition					351:361	cellular fatty acid composition	331:361	cellular fatty acid composition	331:361	We examined this property in 56 Lactobacillus brevis strains isolated from Japanese pickles and also evaluated cellular fatty acid composition and cell-bound exopolysaccharide (EPS-b) production.
24693976	2	60	attach	isolated	281:288	arg1	pickles					304:310	Japanese pickles	295:310	Japanese pickles	295:310	We examined this property in 56 Lactobacillus brevis strains isolated from Japanese pickles and also evaluated cellular fatty acid composition and cell-bound exopolysaccharide (EPS-b) production.
24693976	2	60	attach	isolated	281:288	arg2	strains					273:279	56 Lactobacillus brevis strains	249:279	56 Lactobacillus brevis strains isolated from Japanese pickles	249:310	We examined this property in 56 Lactobacillus brevis strains isolated from Japanese pickles and also evaluated cellular fatty acid composition and cell-bound exopolysaccharide (EPS-b) production.
24693976	8	61	theme	pickle-derived	1374:1387	arg1	brevis					1392:1397	pickle-derived L. brevis	1374:1397	pickle-derived L. brevis	1374:1397	In conclusion, both EPS-b and cellular fatty acid composition play important roles in bile tolerance of pickle-derived L. brevis.
24693976	4	62	theme	bile	640:643	arg1	tolerance					645:653	significantly higher bile tolerance	619:653	significantly higher bile tolerance than nonaggregating strains in MRS medium	619:695	Aggregating strains showed significantly higher bile tolerance than nonaggregating strains in MRS medium, but there was no significant difference in the modified MRS media.
24693976	2	63	theme	brevis	266:271	arg1	strains					273:279	56 Lactobacillus brevis strains	249:279	56 Lactobacillus brevis strains isolated from Japanese pickles	249:310	We examined this property in 56 Lactobacillus brevis strains isolated from Japanese pickles and also evaluated cellular fatty acid composition and cell-bound exopolysaccharide (EPS-b) production.
24693976	3	64	theme	Man	491:493	arg1	medium					519:524	modified de Man - Rogosa - Sharpe (MRS) medium	479:524	modified de Man - Rogosa - Sharpe (MRS) medium (without Tween 80 or sodium acetate)	479:561	The bile tolerance of these strains was significantly lower in modified de Man - Rogosa - Sharpe (MRS) medium (without Tween 80 or sodium acetate) than in standard MRS medium.
24693976	1	65	theme	Bile	157:160	arg1	tolerance					162:170	Bile tolerance	157:170	Bile tolerance	157:170	Bile tolerance is a fundamental ability of probiotic bacteria.
24693976	1	65	theme	Bile	157:160	arg1	ability					189:195	a fundamental ability	175:195	a fundamental ability of probiotic bacteria	175:217	Bile tolerance is a fundamental ability of probiotic bacteria.
24693976	0	66	theme	bile	68:71	arg1	tolerance					73:81	bile tolerance	68:81	bile tolerance in Lactobacillus brevis strains isolated from fermented Japanese pickles	68:154	Cellular fatty acid composition and exopolysaccharide contribute to bile tolerance in Lactobacillus brevis strains isolated from fermented Japanese pickles.
24693976	2	67	theme	Lactobacillus	252:264	arg1	strains					273:279	56 Lactobacillus brevis strains	249:279	56 Lactobacillus brevis strains isolated from Japanese pickles	249:310	We examined this property in 56 Lactobacillus brevis strains isolated from Japanese pickles and also evaluated cellular fatty acid composition and cell-bound exopolysaccharide (EPS-b) production.
24693976	0	68	attach	isolated	115:122	arg1	pickles					148:154	fermented Japanese pickles	129:154	fermented Japanese pickles	129:154	Cellular fatty acid composition and exopolysaccharide contribute to bile tolerance in Lactobacillus brevis strains isolated from fermented Japanese pickles.
24693976	0	68	attach	isolated	115:122	arg2	strains					107:113	Lactobacillus brevis strains	86:113	Lactobacillus brevis strains isolated from fermented Japanese pickles	86:154	Cellular fatty acid composition and exopolysaccharide contribute to bile tolerance in Lactobacillus brevis strains isolated from fermented Japanese pickles.
24693976	4	69	theme	MRS	686:688	arg1	medium					690:695	MRS medium	686:695	MRS medium	686:695	Aggregating strains showed significantly higher bile tolerance than nonaggregating strains in MRS medium, but there was no significant difference in the modified MRS media.
26062117	0	0	theme	exopolysaccharides	80:97	arg1	characterization					60:75	characterization	60:75	characterization	60:75	Effects of cigarette smoke condensate on the production and characterization of exopolysaccharides by Bifidobacterium.
26062117	0	0	theme	exopolysaccharides	80:97	arg1	production					45:54	production	45:54	production	45:54	Effects of cigarette smoke condensate on the production and characterization of exopolysaccharides by Bifidobacterium.
26062117	6	1	theme	carbohydrate	825:836	arg1	composition					838:848	EPSs carbohydrate composition	820:848	EPSs carbohydrate composition	820:848	By comparison with control, CSC was found to be of great impact on EPSs carbohydrate composition.
26062117	6	2	theme	EPSs	820:823	arg1	composition					838:848	EPSs carbohydrate composition	820:848	EPSs carbohydrate composition	820:848	By comparison with control, CSC was found to be of great impact on EPSs carbohydrate composition.
26062117	7	3	theme	Fr-I	880:883	arg1	mass					872:875	The molecular weight mass	851:875	The molecular weight mass of Fr-I	851:883	The molecular weight mass of Fr-I changed from 3.33 × 10(5) g/mol (without CSC) to 2.99 × 10(5) (with CSC).
26062117	8	4	theme	pathological	1130:1141	arg1	disorders					1143:1151	certain pathological disorders	1122:1151	certain pathological disorders	1122:1151	In conclusion, in vitro studies revealed that CSC was directly able to affect the production of metabolites for B. animalis, which could be an essential factor in certain pathological disorders.
26062117	0	5	from	Effects	0:6	arg1	characterization					60:75	characterization	60:75	characterization	60:75	Effects of cigarette smoke condensate on the production and characterization of exopolysaccharides by Bifidobacterium.
26062117	0	5	from	Effects	0:6	arg1	production					45:54	production	45:54	production	45:54	Effects of cigarette smoke condensate on the production and characterization of exopolysaccharides by Bifidobacterium.
26062117	3	6	theme	CSC	508:510	arg1	addition					496:503	anaerobic addition	486:503	anaerobic addition of CSC	486:510	The standard strain of Bifidobacterium animalis was cultured in MRS media under anaerobic addition of CSC.
26062117	1	7	theme	study	134:138	arg1	aim					123:125	The aim	119:125	The aim of the study	119:138	The aim of the study was to investigate the effect of cigarette smoke on the production and characterization of exopolysaccharides (EPSs) produced by Bifidobacterium.
26062117	6	8	from	impact	810:815	arg1	composition					838:848	EPSs carbohydrate composition	820:848	EPSs carbohydrate composition	820:848	By comparison with control, CSC was found to be of great impact on EPSs carbohydrate composition.
26062117	3	9	theme	animalis	445:452	arg1	strain					419:424	The standard strain	406:424	The standard strain of Bifidobacterium animalis	406:452	The standard strain of Bifidobacterium animalis was cultured in MRS media under anaerobic addition of CSC.
26062117	8	10	dep	in	974:975	arg1	vitro					977:981	vitro	977:981	vitro	977:981	In conclusion, in vitro studies revealed that CSC was directly able to affect the production of metabolites for B. animalis, which could be an essential factor in certain pathological disorders.
26062117	6	11	with	comparison	756:765	arg1	control					772:778	control	772:778	control	772:778	By comparison with control, CSC was found to be of great impact on EPSs carbohydrate composition.
26062117	4	12	theme	acetic	611:616	arg1	acid					618:621	acetic acid	611:621	acetic acid	611:621	The results showed that CSC significantly decreased the growth of B. animalis as well as EPSs and acetic acid production.
26062117	4	13	theme	EPSs	602:605	arg1	production					623:632	EPSs and acetic acid production	602:632	the growth of B. animalis as well as EPSs and acetic acid production	565:632	The results showed that CSC significantly decreased the growth of B. animalis as well as EPSs and acetic acid production.
26062117	7	14	theme	molecular	855:863	arg1	mass					872:875	The molecular weight mass	851:875	The molecular weight mass of Fr-I	851:883	The molecular weight mass of Fr-I changed from 3.33 × 10(5) g/mol (without CSC) to 2.99 × 10(5) (with CSC).
26062117	3	15	theme	anaerobic	486:494	arg1	addition					496:503	anaerobic addition	486:503	anaerobic addition of CSC	486:510	The standard strain of Bifidobacterium animalis was cultured in MRS media under anaerobic addition of CSC.
26062117	5	16	theme	molecular	728:736	arg1	determination					738:750	chemical and molecular determination	715:750	chemical and molecular determination	715:750	Furthermore, two EPSs fractions (Fr-I and Fr-II) were isolated and purified for chemical and molecular determination.
26062117	7	17	theme	weight	865:870	arg1	mass					872:875	The molecular weight mass	851:875	The molecular weight mass of Fr-I	851:883	The molecular weight mass of Fr-I changed from 3.33 × 10(5) g/mol (without CSC) to 2.99 × 10(5) (with CSC).
26062117	8	18	dep	B.	1071:1072	arg1	factor					1112:1117	an essential factor	1099:1117	an essential factor in certain pathological disorders	1099:1151	In conclusion, in vitro studies revealed that CSC was directly able to affect the production of metabolites for B. animalis, which could be an essential factor in certain pathological disorders.
26062117	8	18	dep	B.	1071:1072	arg1	which					1084:1088	which	1084:1088	which	1084:1088	In conclusion, in vitro studies revealed that CSC was directly able to affect the production of metabolites for B. animalis, which could be an essential factor in certain pathological disorders.
26062117	8	18	dep	B.	1071:1072	arg1	animalis					1074:1081	B. animalis, which could be an essential factor in certain pathological disorders	1071:1151	animalis	1074:1081	In conclusion, in vitro studies revealed that CSC was directly able to affect the production of metabolites for B. animalis, which could be an essential factor in certain pathological disorders.
26062117	6	19	theme	great	804:808	arg1	impact					810:815	great impact	804:815	great impact on EPSs carbohydrate composition	804:848	By comparison with control, CSC was found to be of great impact on EPSs carbohydrate composition.
26062117	1	20	theme	cigarette	173:181	arg1	smoke					183:187	cigarette smoke	173:187	cigarette smoke	173:187	The aim of the study was to investigate the effect of cigarette smoke on the production and characterization of exopolysaccharides (EPSs) produced by Bifidobacterium.
26062117	8	21	theme	metabolites	1055:1065	arg1	production					1041:1050	the production	1037:1050	the production of metabolites for B. animalis, which could be an essential factor in certain pathological disorders	1037:1151	In conclusion, in vitro studies revealed that CSC was directly able to affect the production of metabolites for B. animalis, which could be an essential factor in certain pathological disorders.
26062117	8	22	theme	in	974:975	arg1	studies					983:989	in vitro studies	974:989	in vitro studies	974:989	In conclusion, in vitro studies revealed that CSC was directly able to affect the production of metabolites for B. animalis, which could be an essential factor in certain pathological disorders.
26062117	1	23	theme	smoke	183:187	arg1	effect					163:168	the effect	159:168	the effect of cigarette smoke on the production and characterization of exopolysaccharides (EPSs) produced by Bifidobacterium	159:283	The aim of the study was to investigate the effect of cigarette smoke on the production and characterization of exopolysaccharides (EPSs) produced by Bifidobacterium.
26062117	0	24	theme	smoke	21:25	arg1	condensate					27:36	cigarette smoke condensate	11:36	cigarette smoke condensate	11:36	Effects of cigarette smoke condensate on the production and characterization of exopolysaccharides by Bifidobacterium.
26062117	2	25	theme	brand	308:312	arg1	Cigarettes					286:295	Cigarettes	286:295	Cigarettes of Shanhua brand (nicotine: 1.1 mg, tar: 11 mg)	286:343	Cigarettes of Shanhua brand (nicotine: 1.1 mg, tar: 11 mg) were utilized to prepare a cigarette smoke condensate (CSC).
26062117	8	26	from	factor	1112:1117	arg1	disorders					1143:1151	certain pathological disorders	1122:1151	certain pathological disorders	1122:1151	In conclusion, in vitro studies revealed that CSC was directly able to affect the production of metabolites for B. animalis, which could be an essential factor in certain pathological disorders.
26062117	2	27	theme	smoke	382:386	arg1	CSC					400:402	CSC	400:402	CSC	400:402	Cigarettes of Shanhua brand (nicotine: 1.1 mg, tar: 11 mg) were utilized to prepare a cigarette smoke condensate (CSC).
26062117	2	27	theme	smoke	382:386	arg1	condensate					388:397	a cigarette smoke condensate	370:397	a cigarette smoke condensate (CSC)	370:403	Cigarettes of Shanhua brand (nicotine: 1.1 mg, tar: 11 mg) were utilized to prepare a cigarette smoke condensate (CSC).
26062117	0	28	theme	cigarette	11:19	arg1	condensate					27:36	cigarette smoke condensate	11:36	cigarette smoke condensate	11:36	Effects of cigarette smoke condensate on the production and characterization of exopolysaccharides by Bifidobacterium.
26062117	2	29	theme	Shanhua	300:306	arg1	brand					308:312	Shanhua brand	300:312	Shanhua brand (nicotine: 1.1 mg, tar: 11 mg)	300:343	Cigarettes of Shanhua brand (nicotine: 1.1 mg, tar: 11 mg) were utilized to prepare a cigarette smoke condensate (CSC).
26062117	2	30	theme	cigarette	372:380	arg1	CSC					400:402	CSC	400:402	CSC	400:402	Cigarettes of Shanhua brand (nicotine: 1.1 mg, tar: 11 mg) were utilized to prepare a cigarette smoke condensate (CSC).
26062117	2	30	theme	cigarette	372:380	arg1	condensate					388:397	a cigarette smoke condensate	370:397	a cigarette smoke condensate (CSC)	370:403	Cigarettes of Shanhua brand (nicotine: 1.1 mg, tar: 11 mg) were utilized to prepare a cigarette smoke condensate (CSC).
26062117	5	31	theme	EPSs	652:655	arg1	Fr-II					677:681	Fr-II	677:681	Fr-II	677:681	Furthermore, two EPSs fractions (Fr-I and Fr-II) were isolated and purified for chemical and molecular determination.
26062117	5	31	theme	EPSs	652:655	arg1	Fr-I					668:671	Fr-I	668:671	Fr-I	668:671	Furthermore, two EPSs fractions (Fr-I and Fr-II) were isolated and purified for chemical and molecular determination.
26062117	5	31	theme	EPSs	652:655	arg1	fractions					657:665	two EPSs fractions	648:665	two EPSs fractions (Fr-I and Fr-II)	648:682	Furthermore, two EPSs fractions (Fr-I and Fr-II) were isolated and purified for chemical and molecular determination.
26062117	0	32	dep	production	45:54	arg1	the					41:43	the	41:43	the	41:43	Effects of cigarette smoke condensate on the production and characterization of exopolysaccharides by Bifidobacterium.
26062117	7	33	with	×	939:939	arg1	CSC					953:955	CSC	953:955	CSC	953:955	The molecular weight mass of Fr-I changed from 3.33 × 10(5) g/mol (without CSC) to 2.99 × 10(5) (with CSC).
26062117	0	34	theme	condensate	27:36	arg1	Effects					0:6	Effects	0:6	Effects of cigarette smoke condensate on the production and characterization of exopolysaccharides by Bifidobacterium	0:116	Effects of cigarette smoke condensate on the production and characterization of exopolysaccharides by Bifidobacterium.
26062117	8	35	theme	essential	1102:1110	arg1	factor					1112:1117	an essential factor	1099:1117	an essential factor in certain pathological disorders	1099:1151	In conclusion, in vitro studies revealed that CSC was directly able to affect the production of metabolites for B. animalis, which could be an essential factor in certain pathological disorders.
26062117	8	35	theme	essential	1102:1110	arg1	which					1084:1088	which	1084:1088	which	1084:1088	In conclusion, in vitro studies revealed that CSC was directly able to affect the production of metabolites for B. animalis, which could be an essential factor in certain pathological disorders.
26062117	5	36	theme	chemical	715:722	arg1	determination					738:750	chemical and molecular determination	715:750	chemical and molecular determination	715:750	Furthermore, two EPSs fractions (Fr-I and Fr-II) were isolated and purified for chemical and molecular determination.
26062117	2	37	dep	brand	308:312	arg1	tar					333:335	tar	333:335	tar	333:335	Cigarettes of Shanhua brand (nicotine: 1.1 mg, tar: 11 mg) were utilized to prepare a cigarette smoke condensate (CSC).
26062117	2	37	dep	brand	308:312	arg1	nicotine					315:322	nicotine	315:322	nicotine	315:322	Cigarettes of Shanhua brand (nicotine: 1.1 mg, tar: 11 mg) were utilized to prepare a cigarette smoke condensate (CSC).
26062117	1	38	dep	production	196:205	arg1	the					192:194	the	192:194	the	192:194	The aim of the study was to investigate the effect of cigarette smoke on the production and characterization of exopolysaccharides (EPSs) produced by Bifidobacterium.
26062117	2	39	dep	nicotine	315:322	arg1	mg					329:330	1.1 mg	325:330	1.1 mg	325:330	Cigarettes of Shanhua brand (nicotine: 1.1 mg, tar: 11 mg) were utilized to prepare a cigarette smoke condensate (CSC).
26062117	2	39	dep	nicotine	315:322	arg1	mg					341:342	11 mg	338:342	11 mg	338:342	Cigarettes of Shanhua brand (nicotine: 1.1 mg, tar: 11 mg) were utilized to prepare a cigarette smoke condensate (CSC).
26062117	1	40	from	effect	163:168	arg1	characterization					211:226	characterization	211:226	characterization	211:226	The aim of the study was to investigate the effect of cigarette smoke on the production and characterization of exopolysaccharides (EPSs) produced by Bifidobacterium.
26062117	1	40	from	effect	163:168	arg1	production					196:205	production	196:205	production	196:205	The aim of the study was to investigate the effect of cigarette smoke on the production and characterization of exopolysaccharides (EPSs) produced by Bifidobacterium.
26062117	8	41	theme	certain	1122:1128	arg1	disorders					1143:1151	certain pathological disorders	1122:1151	certain pathological disorders	1122:1151	In conclusion, in vitro studies revealed that CSC was directly able to affect the production of metabolites for B. animalis, which could be an essential factor in certain pathological disorders.
26062117	7	42	theme	×	903:903	arg1	g/mol					911:915	3.33 × 10(5) g/mol	898:915	3.33 × 10(5) g/mol (without CSC)	898:929	The molecular weight mass of Fr-I changed from 3.33 × 10(5) g/mol (without CSC) to 2.99 × 10(5) (with CSC).
26062117	3	43	theme	Bifidobacterium	429:443	arg1	animalis					445:452	Bifidobacterium animalis	429:452	Bifidobacterium animalis	429:452	The standard strain of Bifidobacterium animalis was cultured in MRS media under anaerobic addition of CSC.
26062117	3	44	theme	standard	410:417	arg1	strain					419:424	The standard strain	406:424	The standard strain of Bifidobacterium animalis	406:452	The standard strain of Bifidobacterium animalis was cultured in MRS media under anaerobic addition of CSC.
26062117	3	45	theme	MRS	470:472	arg1	media					474:478	MRS media	470:478	MRS media	470:478	The standard strain of Bifidobacterium animalis was cultured in MRS media under anaerobic addition of CSC.
26062117	1	46	theme	exopolysaccharides	231:248	arg1	characterization					211:226	characterization	211:226	characterization	211:226	The aim of the study was to investigate the effect of cigarette smoke on the production and characterization of exopolysaccharides (EPSs) produced by Bifidobacterium.
26062117	1	46	theme	exopolysaccharides	231:248	arg1	production					196:205	production	196:205	production	196:205	The aim of the study was to investigate the effect of cigarette smoke on the production and characterization of exopolysaccharides (EPSs) produced by Bifidobacterium.
26062117	5	47	dep	fractions	657:665	arg1	Fr-II					677:681	Fr-II	677:681	Fr-II	677:681	Furthermore, two EPSs fractions (Fr-I and Fr-II) were isolated and purified for chemical and molecular determination.
26062117	5	47	dep	fractions	657:665	arg1	Fr-I					668:671	Fr-I	668:671	Fr-I	668:671	Furthermore, two EPSs fractions (Fr-I and Fr-II) were isolated and purified for chemical and molecular determination.
26062117	5	47	dep	fractions	657:665	arg1	fractions					657:665	two EPSs fractions	648:665	two EPSs fractions (Fr-I and Fr-II)	648:682	Furthermore, two EPSs fractions (Fr-I and Fr-II) were isolated and purified for chemical and molecular determination.
26062117	4	48	theme	animalis	582:589	arg1	growth					569:574	the growth	565:574	the growth of B. animalis as well as EPSs and acetic acid production	565:632	The results showed that CSC significantly decreased the growth of B. animalis as well as EPSs and acetic acid production.
26062117	4	48	theme	animalis	582:589	arg1	production					623:632	EPSs and acetic acid production	602:632	the growth of B. animalis as well as EPSs and acetic acid production	565:632	The results showed that CSC significantly decreased the growth of B. animalis as well as EPSs and acetic acid production.
26062117	2	49	used	utilized	350:357	arg2	Cigarettes					286:295	Cigarettes	286:295	Cigarettes of Shanhua brand (nicotine: 1.1 mg, tar: 11 mg)	286:343	Cigarettes of Shanhua brand (nicotine: 1.1 mg, tar: 11 mg) were utilized to prepare a cigarette smoke condensate (CSC).
26062117	4	50	theme	acid	618:621	arg1	production					623:632	EPSs and acetic acid production	602:632	the growth of B. animalis as well as EPSs and acetic acid production	565:632	The results showed that CSC significantly decreased the growth of B. animalis as well as EPSs and acetic acid production.
29037351	6	0	theme	saphenofemoral	797:810	arg1	junction					812:819	the saphenofemoral junction	793:819	the saphenofemoral junction	793:819	EHIT class 1 was defined as the thrombus extending to the saphenofemoral junction.
29037351	1	1	theme	heat-induced	109:120	arg1	complications					188:200	well-known complications	177:200	well-known complications after superficial endovenous thermoablation	177:244	OBJECTIVE Endovenous heat-induced thrombosis (EHIT) and deep venous thrombosis (DVT) are well-known complications after superficial endovenous thermoablation.
29037351	1	1	theme	heat-induced	109:120	arg1	thrombosis					122:131	OBJECTIVE Endovenous heat-induced thrombosis	88:131	OBJECTIVE Endovenous heat-induced thrombosis (EHIT)	88:138	OBJECTIVE Endovenous heat-induced thrombosis (EHIT) and deep venous thrombosis (DVT) are well-known complications after superficial endovenous thermoablation.
29037351	1	1	theme	heat-induced	109:120	arg1	thrombosis					156:165	deep venous thrombosis	144:165	deep venous thrombosis (DVT)	144:171	OBJECTIVE Endovenous heat-induced thrombosis (EHIT) and deep venous thrombosis (DVT) are well-known complications after superficial endovenous thermoablation.
29037351	1	1	theme	heat-induced	109:120	arg1	EHIT					134:137	EHIT	134:137	EHIT	134:137	OBJECTIVE Endovenous heat-induced thrombosis (EHIT) and deep venous thrombosis (DVT) are well-known complications after superficial endovenous thermoablation.
29037351	5	2	theme	EHIT	668:671	arg1	composite					655:663	the composite	651:663	the composite of EHIT or DVT	651:678	The primary end point was the composite of EHIT or DVT assessed by duplex ultrasound imaging after 1 and 4 weeks.
29037351	5	2	theme	EHIT	668:671	arg1	DVT					676:678	DVT	676:678	DVT	676:678	The primary end point was the composite of EHIT or DVT assessed by duplex ultrasound imaging after 1 and 4 weeks.
29037351	5	2	theme	EHIT	668:671	arg1	EHIT					668:671	EHIT	668:671	EHIT	668:671	The primary end point was the composite of EHIT or DVT assessed by duplex ultrasound imaging after 1 and 4 weeks.
29037351	7	3	theme	venous	846:851	arg1	system					853:858	the deep venous system	837:858	the deep venous system	837:858	Extension into the deep venous system with a cross-sectional area obstruction <50% was considered EHIT class 2.
29037351	8	4	theme	>50	962:964	arg1	%					965:965	%	965:965	%	965:965	EHIT class 3 was defined as >50% cross-sectional area obstruction.
29037351	5	5	theme	end	637:639	arg1	point					641:645	The primary end point	625:645	The primary end point	625:645	The primary end point was the composite of EHIT or DVT assessed by duplex ultrasound imaging after 1 and 4 weeks.
29037351	18	6	from	%	1909:1909	arg1	group					1930:1934	the rivaroxaban group	1914:1934	the rivaroxaban group	1914:1934	Paresthesia was observed in 12.5% in the rivaroxaban group and in 17.8% in the fondaparinux group.
29037351	1	7	theme	well-known	177:186	arg1	complications					188:200	well-known complications	177:200	well-known complications after superficial endovenous thermoablation	177:244	OBJECTIVE Endovenous heat-induced thrombosis (EHIT) and deep venous thrombosis (DVT) are well-known complications after superficial endovenous thermoablation.
29037351	1	7	theme	well-known	177:186	arg1	thrombosis					122:131	OBJECTIVE Endovenous heat-induced thrombosis	88:131	OBJECTIVE Endovenous heat-induced thrombosis (EHIT)	88:138	OBJECTIVE Endovenous heat-induced thrombosis (EHIT) and deep venous thrombosis (DVT) are well-known complications after superficial endovenous thermoablation.
29037351	1	7	theme	well-known	177:186	arg1	thrombosis					156:165	deep venous thrombosis	144:165	deep venous thrombosis (DVT)	144:171	OBJECTIVE Endovenous heat-induced thrombosis (EHIT) and deep venous thrombosis (DVT) are well-known complications after superficial endovenous thermoablation.
29037351	8	8	theme	cross-sectional	967:981	arg1	class					939:943	EHIT class 3	934:945	EHIT class 3	934:945	EHIT class 3 was defined as >50% cross-sectional area obstruction.
29037351	8	8	theme	cross-sectional	967:981	arg1	obstruction					988:998	>50% cross-sectional area obstruction	962:998	>50% cross-sectional area obstruction	962:998	EHIT class 3 was defined as >50% cross-sectional area obstruction.
29037351	13	9	theme	EHIT	1525:1528	arg1	class					1530:1534	EHIT class 2	1525:1536	EHIT class 2 in five (1.3%)	1525:1551	EHIT class 1 was observed in 20 patients (5.1%) and EHIT class 2 in five (1.3%).
29037351	12	10	dep	225	1328:1330	arg1	%					1336:1336	6.2%	1333:1336	6.2%	1333:1336	The primary end point occurred in 13 of 166 (7.8%) and 14 of 225 (6.2%) after 1 week and in 13 of 166 (7.8%) and 15 of 225 (6.7%) after 4 weeks comparing the rivaroxaban and fondaparinux groups (P = .659).
29037351	20	11	theme	oral	2039:2042	arg1	approach					2055:2062	an oral medication approach	2036:2062	an oral medication approach showing no difference in preventing EHIT and DVT compared with fondaparinux, without increased bleeding risk	2036:2171	CONCLUSIONS Rivaroxaban offers an oral medication approach showing no difference in preventing EHIT and DVT compared with fondaparinux, without increased bleeding risk.
29037351	11	12	theme	vein	1261:1264	arg1	EVLA					1231:1234	EVLA	1231:1234	EVLA of the truncal saphenous vein	1231:1264	RESULTS Between February 2009 and December 2015, 391 patients (473 limbs) were treated with EVLA of the truncal saphenous vein.
29037351	5	13	theme	ultrasound	699:708	arg1	imaging					710:716	duplex ultrasound imaging	692:716	duplex ultrasound imaging	692:716	The primary end point was the composite of EHIT or DVT assessed by duplex ultrasound imaging after 1 and 4 weeks.
29037351	2	14	theme	endovenous	324:333	arg1	EVLA					351:354	EVLA	351:354	EVLA	351:354	We investigated the efficacy of rivaroxaban in preventing EHIT and DVT after endovenous laser ablation (EVLA).
29037351	2	14	theme	endovenous	324:333	arg1	ablation					341:348	endovenous laser ablation	324:348	endovenous laser ablation (EVLA)	324:355	We investigated the efficacy of rivaroxaban in preventing EHIT and DVT after endovenous laser ablation (EVLA).
29037351	20	15	theme	increased	2149:2157	arg1	risk					2168:2171	increased bleeding risk	2149:2171	increased bleeding risk	2149:2171	CONCLUSIONS Rivaroxaban offers an oral medication approach showing no difference in preventing EHIT and DVT compared with fondaparinux, without increased bleeding risk.
29037351	1	16	theme	endovenous	220:229	arg1	thermoablation					231:244	superficial endovenous thermoablation	208:244	superficial endovenous thermoablation	208:244	OBJECTIVE Endovenous heat-induced thrombosis (EHIT) and deep venous thrombosis (DVT) are well-known complications after superficial endovenous thermoablation.
29037351	17	17	theme	bleeding	1846:1853	arg1	events					1855:1860	No major bleeding events	1837:1860	No major bleeding events	1837:1860	No major bleeding events were observed.
29037351	12	18	theme	end	1279:1281	arg1	point					1283:1287	The primary end point	1267:1287	The primary end point	1267:1287	The primary end point occurred in 13 of 166 (7.8%) and 14 of 225 (6.2%) after 1 week and in 13 of 166 (7.8%) and 15 of 225 (6.7%) after 4 weeks comparing the rivaroxaban and fondaparinux groups (P = .659).
29037351	15	19	theme	DVT	1606:1608	arg1	one					1614:1616	one	1614:1616	one	1614:1616	The incidence of DVT was one of 166 (0.6%) in the rivaroxaban group and two of 225 (0.9%) in the fondaparinux group (P = .750).
29037351	15	19	theme	DVT	1606:1608	arg1	incidence					1593:1601	The incidence	1589:1601	The incidence of DVT	1589:1608	The incidence of DVT was one of 166 (0.6%) in the rivaroxaban group and two of 225 (0.9%) in the fondaparinux group (P = .750).
29037351	15	19	theme	DVT	1606:1608	arg1	166					1621:1623	166	1621:1623	166	1621:1623	The incidence of DVT was one of 166 (0.6%) in the rivaroxaban group and two of 225 (0.9%) in the fondaparinux group (P = .750).
29037351	19	20	theme	skin	1979:1982	arg1	burns					1984:1988	No skin burns	1976:1988	No skin burns	1976:1988	No skin burns were observed.
29037351	5	21	theme	DVT	676:678	arg1	composite					655:663	the composite	651:663	the composite of EHIT or DVT	651:678	The primary end point was the composite of EHIT or DVT assessed by duplex ultrasound imaging after 1 and 4 weeks.
29037351	5	21	theme	DVT	676:678	arg1	DVT					676:678	DVT	676:678	DVT	676:678	The primary end point was the composite of EHIT or DVT assessed by duplex ultrasound imaging after 1 and 4 weeks.
29037351	5	21	theme	DVT	676:678	arg1	EHIT					668:671	EHIT	668:671	EHIT	668:671	The primary end point was the composite of EHIT or DVT assessed by duplex ultrasound imaging after 1 and 4 weeks.
29037351	1	22	theme	venous	149:154	arg1	complications					188:200	well-known complications	177:200	well-known complications after superficial endovenous thermoablation	177:244	OBJECTIVE Endovenous heat-induced thrombosis (EHIT) and deep venous thrombosis (DVT) are well-known complications after superficial endovenous thermoablation.
29037351	1	22	theme	venous	149:154	arg1	thrombosis					122:131	OBJECTIVE Endovenous heat-induced thrombosis	88:131	OBJECTIVE Endovenous heat-induced thrombosis (EHIT)	88:138	OBJECTIVE Endovenous heat-induced thrombosis (EHIT) and deep venous thrombosis (DVT) are well-known complications after superficial endovenous thermoablation.
29037351	1	22	theme	venous	149:154	arg1	DVT					168:170	DVT	168:170	DVT	168:170	OBJECTIVE Endovenous heat-induced thrombosis (EHIT) and deep venous thrombosis (DVT) are well-known complications after superficial endovenous thermoablation.
29037351	1	22	theme	venous	149:154	arg1	thrombosis					156:165	deep venous thrombosis	144:165	deep venous thrombosis (DVT)	144:171	OBJECTIVE Endovenous heat-induced thrombosis (EHIT) and deep venous thrombosis (DVT) are well-known complications after superficial endovenous thermoablation.
29037351	14	23	theme	EHIT	1570:1573	arg1	class					1575:1579	EHIT class 3 or 4	1570:1586	class	1575:1579	No patients had EHIT class 3 or 4.
29037351	15	24	theme	rivaroxaban	1639:1649	arg1	group					1651:1655	the rivaroxaban group	1635:1655	the rivaroxaban group	1635:1655	The incidence of DVT was one of 166 (0.6%) in the rivaroxaban group and two of 225 (0.9%) in the fondaparinux group (P = .750).
29037351	16	25	dep	occurred	1739:1746	arg1	P =					1826:1828	P =	1826:1828	P =	1826:1828	Minor bleeding events occurred in 17 of 166 patients (10.2%) and in 20 of 225 patients (8.9%), respectively (P = .652).
29037351	5	26	dep	assessed	680:687	arg1	composite					655:663	the composite	651:663	the composite of EHIT or DVT	651:678	The primary end point was the composite of EHIT or DVT assessed by duplex ultrasound imaging after 1 and 4 weeks.
29037351	5	26	dep	assessed	680:687	arg1	DVT					676:678	DVT	676:678	DVT	676:678	The primary end point was the composite of EHIT or DVT assessed by duplex ultrasound imaging after 1 and 4 weeks.
29037351	5	26	dep	assessed	680:687	arg1	EHIT					668:671	EHIT	668:671	EHIT	668:671	The primary end point was the composite of EHIT or DVT assessed by duplex ultrasound imaging after 1 and 4 weeks.
29037351	13	27	from	class	1530:1534	arg1	five					1541:1544	five	1541:1544	five	1541:1544	EHIT class 1 was observed in 20 patients (5.1%) and EHIT class 2 in five (1.3%).
29037351	13	27	from	class	1530:1534	arg1	%					1550:1550	1.3%	1547:1550	1.3%	1547:1550	EHIT class 1 was observed in 20 patients (5.1%) and EHIT class 2 in five (1.3%).
29037351	13	28	theme	EHIT	1473:1476	arg1	class					1478:1482	EHIT class 1	1473:1484	EHIT class 1	1473:1484	EHIT class 1 was observed in 20 patients (5.1%) and EHIT class 2 in five (1.3%).
29037351	9	29	theme	vein	1049:1052	arg1	occlusion					1024:1032	total occlusion	1018:1032	total occlusion of the femoral vein	1018:1052	EHIT class 4 was total occlusion of the femoral vein.
29037351	10	30	theme	end	1069:1071	arg1	minor					1085:1089	minor	1085:1089	minor	1085:1089	The secondary end points were minor or major bleeding, paresthesia, and skin burns.
29037351	10	30	theme	end	1069:1071	arg1	points					1073:1078	The secondary end points	1055:1078	The secondary end points	1055:1078	The secondary end points were minor or major bleeding, paresthesia, and skin burns.
29037351	1	31	theme	Endovenous	98:107	arg1	complications					188:200	well-known complications	177:200	well-known complications after superficial endovenous thermoablation	177:244	OBJECTIVE Endovenous heat-induced thrombosis (EHIT) and deep venous thrombosis (DVT) are well-known complications after superficial endovenous thermoablation.
29037351	1	31	theme	Endovenous	98:107	arg1	thrombosis					122:131	OBJECTIVE Endovenous heat-induced thrombosis	88:131	OBJECTIVE Endovenous heat-induced thrombosis (EHIT)	88:138	OBJECTIVE Endovenous heat-induced thrombosis (EHIT) and deep venous thrombosis (DVT) are well-known complications after superficial endovenous thermoablation.
29037351	1	31	theme	Endovenous	98:107	arg1	thrombosis					156:165	deep venous thrombosis	144:165	deep venous thrombosis (DVT)	144:171	OBJECTIVE Endovenous heat-induced thrombosis (EHIT) and deep venous thrombosis (DVT) are well-known complications after superficial endovenous thermoablation.
29037351	1	31	theme	Endovenous	98:107	arg1	EHIT					134:137	EHIT	134:137	EHIT	134:137	OBJECTIVE Endovenous heat-induced thrombosis (EHIT) and deep venous thrombosis (DVT) are well-known complications after superficial endovenous thermoablation.
29037351	13	32	located	observed	1490:1497	arg1	%					1518:1518	5.1%	1515:1518	5.1%	1515:1518	EHIT class 1 was observed in 20 patients (5.1%) and EHIT class 2 in five (1.3%).
29037351	13	32	located	observed	1490:1497	arg1	class					1530:1534	EHIT class 2	1525:1536	EHIT class 2 in five (1.3%)	1525:1551	EHIT class 1 was observed in 20 patients (5.1%) and EHIT class 2 in five (1.3%).
29037351	13	32	located	observed	1490:1497	arg2	class					1478:1482	EHIT class 1	1473:1484	EHIT class 1	1473:1484	EHIT class 1 was observed in 20 patients (5.1%) and EHIT class 2 in five (1.3%).
29037351	13	32	located	observed	1490:1497	arg1	patients					1505:1512	20 patients	1502:1512	20 patients (5.1%)	1502:1519	EHIT class 1 was observed in 20 patients (5.1%) and EHIT class 2 in five (1.3%).
29037351	3	33	theme	class	461:465	arg1	C2					467:468	truncal varicosis class C2	443:468	truncal varicosis class C2	443:468	METHODS We retrospectively analyzed a consecutive series of patients presenting with truncal varicosis class C2 to C6 undergoing EVLA.
29037351	14	34	theme	No	1554:1555	arg1	patients					1557:1564	No patients	1554:1564	No patients	1554:1564	No patients had EHIT class 3 or 4.
29037351	7	35	theme	EHIT	920:923	arg1	class					925:929	EHIT class 2	920:931	EHIT class 2	920:931	Extension into the deep venous system with a cross-sectional area obstruction <50% was considered EHIT class 2.
29037351	12	36	theme	fondaparinux	1441:1452	arg1	groups					1454:1459	the rivaroxaban and fondaparinux groups	1421:1459	groups	1454:1459	The primary end point occurred in 13 of 166 (7.8%) and 14 of 225 (6.2%) after 1 week and in 13 of 166 (7.8%) and 15 of 225 (6.7%) after 4 weeks comparing the rivaroxaban and fondaparinux groups (P = .659).
29037351	12	36	theme	fondaparinux	1441:1452	arg1	P =					1462:1464	P =	1462:1464	P =	1462:1464	The primary end point occurred in 13 of 166 (7.8%) and 14 of 225 (6.2%) after 1 week and in 13 of 166 (7.8%) and 15 of 225 (6.7%) after 4 weeks comparing the rivaroxaban and fondaparinux groups (P = .659).
29037351	3	37	theme	consecutive	396:406	arg1	series					408:413	a consecutive series	394:413	a consecutive series of patients presenting with truncal varicosis class C2 to C6 undergoing EVLA	394:490	METHODS We retrospectively analyzed a consecutive series of patients presenting with truncal varicosis class C2 to C6 undergoing EVLA.
29037351	3	38	theme	truncal	443:449	arg1	C2					467:468	truncal varicosis class C2	443:468	truncal varicosis class C2	443:468	METHODS We retrospectively analyzed a consecutive series of patients presenting with truncal varicosis class C2 to C6 undergoing EVLA.
29037351	7	39	with	Extension	822:830	arg1	area					883:886	a cross-sectional area	865:886	a cross-sectional area obstruction <50%	865:903	Extension into the deep venous system with a cross-sectional area obstruction <50% was considered EHIT class 2.
29037351	12	40	dep	166	1307:1309	arg1	%					1315:1315	7.8%	1312:1315	7.8%	1312:1315	The primary end point occurred in 13 of 166 (7.8%) and 14 of 225 (6.2%) after 1 week and in 13 of 166 (7.8%) and 15 of 225 (6.7%) after 4 weeks comparing the rivaroxaban and fondaparinux groups (P = .659).
29037351	0	41	theme	endovenous	61:70	arg1	ablation					78:85	endovenous laser ablation	61:85	endovenous laser ablation	61:85	Rivaroxaban versus fondaparinux for thromboprophylaxis after endovenous laser ablation.
29037351	12	42	dep	225	1386:1388	arg1	%					1394:1394	6.7%	1391:1394	6.7%	1391:1394	The primary end point occurred in 13 of 166 (7.8%) and 14 of 225 (6.2%) after 1 week and in 13 of 166 (7.8%) and 15 of 225 (6.7%) after 4 weeks comparing the rivaroxaban and fondaparinux groups (P = .659).
29037351	6	43	theme	EHIT	739:742	arg1	class					744:748	EHIT class 1	739:750	EHIT class 1	739:750	EHIT class 1 was defined as the thrombus extending to the saphenofemoral junction.
29037351	6	43	theme	EHIT	739:742	arg1	thrombus					771:778	the thrombus	767:778	the thrombus extending to the saphenofemoral junction	767:819	EHIT class 1 was defined as the thrombus extending to the saphenofemoral junction.
29037351	4	44	theme	consecutive	607:617	arg1	days					619:622	3 consecutive days	605:622	3 consecutive days	605:622	After EVLA, all patients received oral rivaroxaban (10 mg) or subcutaneous fondaparinux (2.5 mg) once daily for 3 consecutive days.
29037351	7	45	theme	%	903:903	arg1	area					883:886	a cross-sectional area	865:886	a cross-sectional area obstruction <50%	865:903	Extension into the deep venous system with a cross-sectional area obstruction <50% was considered EHIT class 2.
29037351	16	46	theme	Minor	1717:1721	arg1	events					1732:1737	Minor bleeding events	1717:1737	Minor bleeding events	1717:1737	Minor bleeding events occurred in 17 of 166 patients (10.2%) and in 20 of 225 patients (8.9%), respectively (P = .652).
29037351	18	47	located	observed	1893:1900	arg2	Paresthesia					1877:1887	Paresthesia	1877:1887	Paresthesia	1877:1887	Paresthesia was observed in 12.5% in the rivaroxaban group and in 17.8% in the fondaparinux group.
29037351	18	47	located	observed	1893:1900	arg1	%					1909:1909	12.5%	1905:1909	12.5% in the rivaroxaban group	1905:1934	Paresthesia was observed in 12.5% in the rivaroxaban group and in 17.8% in the fondaparinux group.
29037351	8	48	theme	%	965:965	arg1	class					939:943	EHIT class 3	934:945	EHIT class 3	934:945	EHIT class 3 was defined as >50% cross-sectional area obstruction.
29037351	8	48	theme	%	965:965	arg1	obstruction					988:998	>50% cross-sectional area obstruction	962:998	>50% cross-sectional area obstruction	962:998	EHIT class 3 was defined as >50% cross-sectional area obstruction.
29037351	15	49	from	one	1614:1616	arg1	P =					1706:1708	P = .750	1706:1713	P = .750	1706:1713	The incidence of DVT was one of 166 (0.6%) in the rivaroxaban group and two of 225 (0.9%) in the fondaparinux group (P = .750).
29037351	15	49	from	one	1614:1616	arg1	group					1699:1703	the fondaparinux group	1682:1703	the fondaparinux group (P = .750)	1682:1714	The incidence of DVT was one of 166 (0.6%) in the rivaroxaban group and two of 225 (0.9%) in the fondaparinux group (P = .750).
29037351	5	50	theme	primary	629:635	arg1	point					641:645	The primary end point	625:645	The primary end point	625:645	The primary end point was the composite of EHIT or DVT assessed by duplex ultrasound imaging after 1 and 4 weeks.
29037351	20	51	theme	bleeding	2159:2166	arg1	risk					2168:2171	increased bleeding risk	2149:2171	increased bleeding risk	2149:2171	CONCLUSIONS Rivaroxaban offers an oral medication approach showing no difference in preventing EHIT and DVT compared with fondaparinux, without increased bleeding risk.
29037351	8	52	theme	area	983:986	arg1	class					939:943	EHIT class 3	934:945	EHIT class 3	934:945	EHIT class 3 was defined as >50% cross-sectional area obstruction.
29037351	8	52	theme	area	983:986	arg1	obstruction					988:998	>50% cross-sectional area obstruction	962:998	>50% cross-sectional area obstruction	962:998	EHIT class 3 was defined as >50% cross-sectional area obstruction.
29037351	15	53	theme	166	1621:1623	arg1	%					1629:1629	0.6%	1626:1629	0.6%	1626:1629	The incidence of DVT was one of 166 (0.6%) in the rivaroxaban group and two of 225 (0.9%) in the fondaparinux group (P = .750).
29037351	15	53	theme	166	1621:1623	arg1	one					1614:1616	one	1614:1616	one	1614:1616	The incidence of DVT was one of 166 (0.6%) in the rivaroxaban group and two of 225 (0.9%) in the fondaparinux group (P = .750).
29037351	15	53	theme	166	1621:1623	arg1	incidence					1593:1601	The incidence	1589:1601	The incidence of DVT	1589:1608	The incidence of DVT was one of 166 (0.6%) in the rivaroxaban group and two of 225 (0.9%) in the fondaparinux group (P = .750).
29037351	15	53	theme	166	1621:1623	arg1	166					1621:1623	166	1621:1623	166	1621:1623	The incidence of DVT was one of 166 (0.6%) in the rivaroxaban group and two of 225 (0.9%) in the fondaparinux group (P = .750).
29037351	16	54	theme	patients	1761:1768	arg1	17					1751:1752	17	1751:1752	17	1751:1752	Minor bleeding events occurred in 17 of 166 patients (10.2%) and in 20 of 225 patients (8.9%), respectively (P = .652).
29037351	16	54	theme	patients	1761:1768	arg1	patients					1761:1768	166 patients	1757:1768	166 patients (10.2%)	1757:1776	Minor bleeding events occurred in 17 of 166 patients (10.2%) and in 20 of 225 patients (8.9%), respectively (P = .652).
29037351	16	54	theme	patients	1761:1768	arg1	%					1775:1775	10.2%	1771:1775	10.2%	1771:1775	Minor bleeding events occurred in 17 of 166 patients (10.2%) and in 20 of 225 patients (8.9%), respectively (P = .652).
29037351	15	55	from	166	1621:1623	arg1	group					1651:1655	the rivaroxaban group	1635:1655	the rivaroxaban group	1635:1655	The incidence of DVT was one of 166 (0.6%) in the rivaroxaban group and two of 225 (0.9%) in the fondaparinux group (P = .750).
29037351	7	56	theme	deep	841:844	arg1	system					853:858	the deep venous system	837:858	the deep venous system	837:858	Extension into the deep venous system with a cross-sectional area obstruction <50% was considered EHIT class 2.
29037351	18	57	theme	fondaparinux	1956:1967	arg1	group					1969:1973	the fondaparinux group	1952:1973	the fondaparinux group	1952:1973	Paresthesia was observed in 12.5% in the rivaroxaban group and in 17.8% in the fondaparinux group.
29037351	20	58	dep	CONCLUSIONS	2005:2015	arg1	offers					2029:2034	offers	2029:2034	offers an oral medication approach showing no difference in preventing EHIT and DVT compared with fondaparinux, without increased bleeding risk	2029:2171	CONCLUSIONS Rivaroxaban offers an oral medication approach showing no difference in preventing EHIT and DVT compared with fondaparinux, without increased bleeding risk.
29037351	11	59	theme	saphenous	1251:1259	arg1	vein					1261:1264	the truncal saphenous vein	1239:1264	the truncal saphenous vein	1239:1264	RESULTS Between February 2009 and December 2015, 391 patients (473 limbs) were treated with EVLA of the truncal saphenous vein.
29037351	5	60	theme	duplex	692:697	arg1	imaging					710:716	duplex ultrasound imaging	692:716	duplex ultrasound imaging	692:716	The primary end point was the composite of EHIT or DVT assessed by duplex ultrasound imaging after 1 and 4 weeks.
29037351	2	61	theme	laser	335:339	arg1	EVLA					351:354	EVLA	351:354	EVLA	351:354	We investigated the efficacy of rivaroxaban in preventing EHIT and DVT after endovenous laser ablation (EVLA).
29037351	2	61	theme	laser	335:339	arg1	ablation					341:348	endovenous laser ablation	324:348	endovenous laser ablation (EVLA)	324:355	We investigated the efficacy of rivaroxaban in preventing EHIT and DVT after endovenous laser ablation (EVLA).
29037351	1	62	theme	superficial	208:218	arg1	thermoablation					231:244	superficial endovenous thermoablation	208:244	superficial endovenous thermoablation	208:244	OBJECTIVE Endovenous heat-induced thrombosis (EHIT) and deep venous thrombosis (DVT) are well-known complications after superficial endovenous thermoablation.
29037351	12	63	theme	rivaroxaban	1425:1435	arg1	groups					1454:1459	the rivaroxaban and fondaparinux groups	1421:1459	groups	1454:1459	The primary end point occurred in 13 of 166 (7.8%) and 14 of 225 (6.2%) after 1 week and in 13 of 166 (7.8%) and 15 of 225 (6.7%) after 4 weeks comparing the rivaroxaban and fondaparinux groups (P = .659).
29037351	12	63	theme	rivaroxaban	1425:1435	arg1	P =					1462:1464	P =	1462:1464	P =	1462:1464	The primary end point occurred in 13 of 166 (7.8%) and 14 of 225 (6.2%) after 1 week and in 13 of 166 (7.8%) and 15 of 225 (6.7%) after 4 weeks comparing the rivaroxaban and fondaparinux groups (P = .659).
29037351	17	64	theme	major	1840:1844	arg1	events					1855:1860	No major bleeding events	1837:1860	No major bleeding events	1837:1860	No major bleeding events were observed.
29037351	12	65	theme	primary	1271:1277	arg1	point					1283:1287	The primary end point	1267:1287	The primary end point	1267:1287	The primary end point occurred in 13 of 166 (7.8%) and 14 of 225 (6.2%) after 1 week and in 13 of 166 (7.8%) and 15 of 225 (6.7%) after 4 weeks comparing the rivaroxaban and fondaparinux groups (P = .659).
29037351	14	66	contain	had	1566:1568	arg1	patients					1557:1564	No patients	1554:1564	No patients	1554:1564	No patients had EHIT class 3 or 4.
29037351	14	66	contain	had	1566:1568	arg2	4					1586:1586	4	1586:1586	4	1586:1586	No patients had EHIT class 3 or 4.
29037351	14	66	contain	had	1566:1568	arg2	class					1575:1579	EHIT class 3 or 4	1570:1586	class	1575:1579	No patients had EHIT class 3 or 4.
29037351	9	67	theme	EHIT	1001:1004	arg1	class					1006:1010	EHIT class 4	1001:1012	EHIT class 4	1001:1012	EHIT class 4 was total occlusion of the femoral vein.
29037351	1	68	theme	deep	144:147	arg1	complications					188:200	well-known complications	177:200	well-known complications after superficial endovenous thermoablation	177:244	OBJECTIVE Endovenous heat-induced thrombosis (EHIT) and deep venous thrombosis (DVT) are well-known complications after superficial endovenous thermoablation.
29037351	1	68	theme	deep	144:147	arg1	thrombosis					122:131	OBJECTIVE Endovenous heat-induced thrombosis	88:131	OBJECTIVE Endovenous heat-induced thrombosis (EHIT)	88:138	OBJECTIVE Endovenous heat-induced thrombosis (EHIT) and deep venous thrombosis (DVT) are well-known complications after superficial endovenous thermoablation.
29037351	1	68	theme	deep	144:147	arg1	DVT					168:170	DVT	168:170	DVT	168:170	OBJECTIVE Endovenous heat-induced thrombosis (EHIT) and deep venous thrombosis (DVT) are well-known complications after superficial endovenous thermoablation.
29037351	1	68	theme	deep	144:147	arg1	thrombosis					156:165	deep venous thrombosis	144:165	deep venous thrombosis (DVT)	144:171	OBJECTIVE Endovenous heat-induced thrombosis (EHIT) and deep venous thrombosis (DVT) are well-known complications after superficial endovenous thermoablation.
29037351	18	69	theme	rivaroxaban	1918:1928	arg1	group					1930:1934	the rivaroxaban group	1914:1934	the rivaroxaban group	1914:1934	Paresthesia was observed in 12.5% in the rivaroxaban group and in 17.8% in the fondaparinux group.
29037351	2	70	theme	rivaroxaban	279:289	arg1	efficacy					267:274	the efficacy	263:274	the efficacy of rivaroxaban	263:289	We investigated the efficacy of rivaroxaban in preventing EHIT and DVT after endovenous laser ablation (EVLA).
29037351	4	71	theme	oral	527:530	arg1	10 mg					545:549	10 mg	545:549	10 mg	545:549	After EVLA, all patients received oral rivaroxaban (10 mg) or subcutaneous fondaparinux (2.5 mg) once daily for 3 consecutive days.
29037351	4	71	theme	oral	527:530	arg1	rivaroxaban					532:542	oral rivaroxaban	527:542	oral rivaroxaban (10 mg)	527:550	After EVLA, all patients received oral rivaroxaban (10 mg) or subcutaneous fondaparinux (2.5 mg) once daily for 3 consecutive days.
29037351	9	72	theme	total	1018:1022	arg1	occlusion					1024:1032	total occlusion	1018:1032	total occlusion of the femoral vein	1018:1052	EHIT class 4 was total occlusion of the femoral vein.
29037351	12	73	dep	166	1365:1367	arg1	%					1373:1373	7.8%	1370:1373	7.8%	1370:1373	The primary end point occurred in 13 of 166 (7.8%) and 14 of 225 (6.2%) after 1 week and in 13 of 166 (7.8%) and 15 of 225 (6.7%) after 4 weeks comparing the rivaroxaban and fondaparinux groups (P = .659).
29037351	20	74	theme	medication	2044:2053	arg1	approach					2055:2062	an oral medication approach	2036:2062	an oral medication approach showing no difference in preventing EHIT and DVT compared with fondaparinux, without increased bleeding risk	2036:2171	CONCLUSIONS Rivaroxaban offers an oral medication approach showing no difference in preventing EHIT and DVT compared with fondaparinux, without increased bleeding risk.
29037351	16	75	theme	bleeding	1723:1730	arg1	events					1732:1737	Minor bleeding events	1717:1737	Minor bleeding events	1717:1737	Minor bleeding events occurred in 17 of 166 patients (10.2%) and in 20 of 225 patients (8.9%), respectively (P = .652).
29037351	9	76	theme	femoral	1041:1047	arg1	vein					1049:1052	the femoral vein	1037:1052	the femoral vein	1037:1052	EHIT class 4 was total occlusion of the femoral vein.
29037351	1	77	theme	OBJECTIVE	88:96	arg1	complications					188:200	well-known complications	177:200	well-known complications after superficial endovenous thermoablation	177:244	OBJECTIVE Endovenous heat-induced thrombosis (EHIT) and deep venous thrombosis (DVT) are well-known complications after superficial endovenous thermoablation.
29037351	1	77	theme	OBJECTIVE	88:96	arg1	thrombosis					122:131	OBJECTIVE Endovenous heat-induced thrombosis	88:131	OBJECTIVE Endovenous heat-induced thrombosis (EHIT)	88:138	OBJECTIVE Endovenous heat-induced thrombosis (EHIT) and deep venous thrombosis (DVT) are well-known complications after superficial endovenous thermoablation.
29037351	1	77	theme	OBJECTIVE	88:96	arg1	thrombosis					156:165	deep venous thrombosis	144:165	deep venous thrombosis (DVT)	144:171	OBJECTIVE Endovenous heat-induced thrombosis (EHIT) and deep venous thrombosis (DVT) are well-known complications after superficial endovenous thermoablation.
29037351	1	77	theme	OBJECTIVE	88:96	arg1	EHIT					134:137	EHIT	134:137	EHIT	134:137	OBJECTIVE Endovenous heat-induced thrombosis (EHIT) and deep venous thrombosis (DVT) are well-known complications after superficial endovenous thermoablation.
29037351	18	78	from	%	1947:1947	arg1	group					1969:1973	the fondaparinux group	1952:1973	the fondaparinux group	1952:1973	Paresthesia was observed in 12.5% in the rivaroxaban group and in 17.8% in the fondaparinux group.
29037351	11	79	theme	truncal	1243:1249	arg1	vein					1261:1264	the truncal saphenous vein	1239:1264	the truncal saphenous vein	1239:1264	RESULTS Between February 2009 and December 2015, 391 patients (473 limbs) were treated with EVLA of the truncal saphenous vein.
29037351	10	80	theme	secondary	1059:1067	arg1	minor					1085:1089	minor	1085:1089	minor	1085:1089	The secondary end points were minor or major bleeding, paresthesia, and skin burns.
29037351	10	80	theme	secondary	1059:1067	arg1	points					1073:1078	The secondary end points	1055:1078	The secondary end points	1055:1078	The secondary end points were minor or major bleeding, paresthesia, and skin burns.
29037351	15	81	theme	fondaparinux	1686:1697	arg1	P =					1706:1708	P = .750	1706:1713	P = .750	1706:1713	The incidence of DVT was one of 166 (0.6%) in the rivaroxaban group and two of 225 (0.9%) in the fondaparinux group (P = .750).
29037351	15	81	theme	fondaparinux	1686:1697	arg1	group					1699:1703	the fondaparinux group	1682:1703	the fondaparinux group (P = .750)	1682:1714	The incidence of DVT was one of 166 (0.6%) in the rivaroxaban group and two of 225 (0.9%) in the fondaparinux group (P = .750).
29037351	3	82	theme	patients	418:425	arg1	series					408:413	a consecutive series	394:413	a consecutive series of patients presenting with truncal varicosis class C2 to C6 undergoing EVLA	394:490	METHODS We retrospectively analyzed a consecutive series of patients presenting with truncal varicosis class C2 to C6 undergoing EVLA.
29037351	3	83	dep	retrospectively	369:383	arg1	analyzed					385:392	analyzed	385:392	retrospectively analyzed a consecutive series of patients presenting with truncal varicosis class C2 to C6 undergoing EVLA	369:490	METHODS We retrospectively analyzed a consecutive series of patients presenting with truncal varicosis class C2 to C6 undergoing EVLA.
29037351	19	84	theme	No	1976:1977	arg1	burns					1984:1988	No skin burns	1976:1988	No skin burns	1976:1988	No skin burns were observed.
29037351	3	85	theme	varicosis	451:459	arg1	C2					467:468	truncal varicosis class C2	443:468	truncal varicosis class C2	443:468	METHODS We retrospectively analyzed a consecutive series of patients presenting with truncal varicosis class C2 to C6 undergoing EVLA.
29037351	3	86	dep	METHODS	358:364	arg1	retrospectively					369:383	retrospectively	369:383	retrospectively analyzed a consecutive series of patients presenting with truncal varicosis class C2 to C6 undergoing EVLA	369:490	METHODS We retrospectively analyzed a consecutive series of patients presenting with truncal varicosis class C2 to C6 undergoing EVLA.
29037351	0	87	theme	laser	72:76	arg1	ablation					78:85	endovenous laser ablation	61:85	endovenous laser ablation	61:85	Rivaroxaban versus fondaparinux for thromboprophylaxis after endovenous laser ablation.
29037351	13	88	from	patients	1505:1512	arg1	five					1541:1544	five	1541:1544	five	1541:1544	EHIT class 1 was observed in 20 patients (5.1%) and EHIT class 2 in five (1.3%).
29037351	13	88	from	patients	1505:1512	arg1	%					1550:1550	1.3%	1547:1550	1.3%	1547:1550	EHIT class 1 was observed in 20 patients (5.1%) and EHIT class 2 in five (1.3%).
29037351	4	89	theme	subcutaneous	555:566	arg1	2.5 mg					582:587	2.5 mg	582:587	2.5 mg	582:587	After EVLA, all patients received oral rivaroxaban (10 mg) or subcutaneous fondaparinux (2.5 mg) once daily for 3 consecutive days.
29037351	4	89	theme	subcutaneous	555:566	arg1	fondaparinux					568:579	subcutaneous fondaparinux	555:579	subcutaneous fondaparinux (2.5 mg)	555:588	After EVLA, all patients received oral rivaroxaban (10 mg) or subcutaneous fondaparinux (2.5 mg) once daily for 3 consecutive days.
29037351	15	90	theme	225	1668:1670	arg1	225					1668:1670	225	1668:1670	225 (0.9%)	1668:1677	The incidence of DVT was one of 166 (0.6%) in the rivaroxaban group and two of 225 (0.9%) in the fondaparinux group (P = .750).
29037351	15	90	theme	225	1668:1670	arg1	%					1676:1676	0.9%	1673:1676	0.9%	1673:1676	The incidence of DVT was one of 166 (0.6%) in the rivaroxaban group and two of 225 (0.9%) in the fondaparinux group (P = .750).
29037351	15	90	theme	225	1668:1670	arg1	two					1661:1663	two	1661:1663	two	1661:1663	The incidence of DVT was one of 166 (0.6%) in the rivaroxaban group and two of 225 (0.9%) in the fondaparinux group (P = .750).
29037351	7	91	theme	obstruction <50	888:902	arg1	%					903:903	%	903:903	%	903:903	Extension into the deep venous system with a cross-sectional area obstruction <50% was considered EHIT class 2.
29037351	8	92	theme	EHIT	934:937	arg1	class					939:943	EHIT class 3	934:945	EHIT class 3	934:945	EHIT class 3 was defined as >50% cross-sectional area obstruction.
29037351	8	92	theme	EHIT	934:937	arg1	obstruction					988:998	>50% cross-sectional area obstruction	962:998	>50% cross-sectional area obstruction	962:998	EHIT class 3 was defined as >50% cross-sectional area obstruction.
29037351	7	93	theme	cross-sectional	867:881	arg1	area					883:886	a cross-sectional area	865:886	a cross-sectional area obstruction <50%	865:903	Extension into the deep venous system with a cross-sectional area obstruction <50% was considered EHIT class 2.
29037351	10	94	theme	skin	1127:1130	arg1	burns					1132:1136	skin burns	1127:1136	skin burns	1127:1136	The secondary end points were minor or major bleeding, paresthesia, and skin burns.
28034827	0	0	theme	composition	78:88	arg1	Effect					68:73	Effect	68:73	Fabrication and properties of polyvinyl alcohol/starch blend films: Effect of composition and humidity.	0:102	Fabrication and properties of polyvinyl alcohol/starch blend films: Effect of composition and humidity.
28034827	5	1	theme	films	601:605	arg1	surface					580:586	The fracture surface	567:586	The fracture surface of the blend films	567:605	The fracture surface of the blend films exhibited rough surface, suggesting the tough fracture.
28034827	5	2	theme	tough	647:651	arg1	fracture					653:660	the tough fracture	643:660	the tough fracture	643:660	The fracture surface of the blend films exhibited rough surface, suggesting the tough fracture.
28034827	2	3	theme	resulting	319:327	arg1	blends					329:334	the resulting blends	315:334	the resulting blends	315:334	The effect of the composition and relative humidity (RH) on the structure and properties of the resulting blends were investigated.
28034827	10	4	theme	improved	1295:1302	arg1	flexibility					1304:1314	the improved flexibility	1291:1314	the improved flexibility	1291:1314	The tensile strength and Young's modulus decreased with the increase of RH, while the elongation at break was enhanced dramatically, indicating the improved flexibility.
28034827	3	5	theme	components	470:479	arg1	compatibility					445:457	the compatibility	441:457	the compatibility of the two components	441:479	OH groups on starch and PVA formed hydrogen bonding interactions, which could improve the compatibility of the two components.
28034827	7	6	theme	resulting	799:807	arg1	blends					809:814	the resulting blends	795:814	the resulting blends	795:814	With the increase of RH, the water uptake at equilibrium of the resulting blends increased.
28034827	4	7	theme	crystallinity	525:537	arg1	degree					515:520	the degree	511:520	the degree of crystallinity of PVA component	511:554	With the increase of starch, the degree of crystallinity of PVA component decreased.
28034827	0	8	theme	humidity	94:101	arg1	Effect					68:73	Effect	68:73	Fabrication and properties of polyvinyl alcohol/starch blend films: Effect of composition and humidity.	0:102	Fabrication and properties of polyvinyl alcohol/starch blend films: Effect of composition and humidity.
28034827	9	9	theme	9MPa	1086:1089	arg1	strength					1074:1081	tensile strength	1066:1081	tensile strength of 9MPa, which was superior to the commonly LDPE package films	1066:1144	However, at 50% starch content, the flexibility of the blend films was still high, with the elongation at break more than 1000% and tensile strength of 9MPa, which was superior to the commonly LDPE package films.
28034827	9	9	theme	9MPa	1086:1089	arg1	break					1040:1044	break	1040:1044	break more than 1000%	1040:1060	However, at 50% starch content, the flexibility of the blend films was still high, with the elongation at break more than 1000% and tensile strength of 9MPa, which was superior to the commonly LDPE package films.
28034827	5	10	theme	rough	617:621	arg1	surface					623:629	rough surface	617:629	rough surface	617:629	The fracture surface of the blend films exhibited rough surface, suggesting the tough fracture.
28034827	7	11	theme	blends	809:814	arg1	uptake					770:775	the water uptake	760:775	the water uptake at equilibrium of the resulting blends	760:814	With the increase of RH, the water uptake at equilibrium of the resulting blends increased.
28034827	4	12	theme	component	546:554	arg1	crystallinity					525:537	crystallinity	525:537	crystallinity of PVA component	525:554	With the increase of starch, the degree of crystallinity of PVA component decreased.
28034827	9	13	theme	tensile	1066:1072	arg1	strength					1074:1081	tensile strength	1066:1081	tensile strength of 9MPa, which was superior to the commonly LDPE package films	1066:1144	However, at 50% starch content, the flexibility of the blend films was still high, with the elongation at break more than 1000% and tensile strength of 9MPa, which was superior to the commonly LDPE package films.
28034827	2	14	theme	humidity	266:273	arg1	effect					227:232	The effect	223:232	The effect of the composition and relative humidity (RH) on the structure and properties of the resulting blends	223:334	The effect of the composition and relative humidity (RH) on the structure and properties of the resulting blends were investigated.
28034827	4	15	theme	PVA	542:544	arg1	component					546:554	PVA component	542:554	PVA component	542:554	With the increase of starch, the degree of crystallinity of PVA component decreased.
28034827	5	16	theme	fracture	571:578	arg1	surface					580:586	The fracture surface	567:586	The fracture surface of the blend films	567:605	The fracture surface of the blend films exhibited rough surface, suggesting the tough fracture.
28034827	2	17	theme	relative	257:264	arg1	humidity					266:273	relative humidity	257:273	relative humidity (RH)	257:278	The effect of the composition and relative humidity (RH) on the structure and properties of the resulting blends were investigated.
28034827	2	17	theme	relative	257:264	arg1	RH					276:277	RH	276:277	RH	276:277	The effect of the composition and relative humidity (RH) on the structure and properties of the resulting blends were investigated.
28034827	9	18	from	break	1040:1044	arg1	elongation					1026:1035	the elongation	1022:1035	the elongation at break more than 1000% and tensile strength of 9MPa, which was superior to the commonly LDPE package films	1022:1144	However, at 50% starch content, the flexibility of the blend films was still high, with the elongation at break more than 1000% and tensile strength of 9MPa, which was superior to the commonly LDPE package films.
28034827	1	19	theme	blend	149:153	arg1	films					155:159	starch/polyvinyl alcohol (PVA) blend films	118:159	starch/polyvinyl alcohol (PVA) blend films with different compositions	118:187	In this work, starch/polyvinyl alcohol (PVA) blend films with different compositions were prepared by melt processing.
28034827	9	20	theme	blend	989:993	arg1	films					995:999	the blend films	985:999	the blend films	985:999	However, at 50% starch content, the flexibility of the blend films was still high, with the elongation at break more than 1000% and tensile strength of 9MPa, which was superior to the commonly LDPE package films.
28034827	8	21	from	break	863:867	arg1	elongation					849:858	elongation at break	849:867	elongation at break	849:867	The tensile strength, elongation at break and Young's modulus decreased with increasing content of starch.
28034827	8	21	from	break	863:867	arg1	at					860:861	elongation at break	849:867	elongation at break	849:867	The tensile strength, elongation at break and Young's modulus decreased with increasing content of starch.
28034827	3	22	theme	OH	355:356	arg1	groups					358:363	OH groups	355:363	OH groups on starch and PVA	355:381	OH groups on starch and PVA formed hydrogen bonding interactions, which could improve the compatibility of the two components.
28034827	3	23	from	groups	358:363	arg1	starch					368:373	starch	368:373	starch	368:373	OH groups on starch and PVA formed hydrogen bonding interactions, which could improve the compatibility of the two components.
28034827	3	23	from	groups	358:363	arg1	PVA					379:381	PVA	379:381	PVA	379:381	OH groups on starch and PVA formed hydrogen bonding interactions, which could improve the compatibility of the two components.
28034827	8	24	theme	starch	926:931	arg1	content					915:921	content	915:921	content of starch	915:931	The tensile strength, elongation at break and Young's modulus decreased with increasing content of starch.
28034827	9	25	theme	films	995:999	arg1	high					1011:1014	high	1011:1014	high	1011:1014	However, at 50% starch content, the flexibility of the blend films was still high, with the elongation at break more than 1000% and tensile strength of 9MPa, which was superior to the commonly LDPE package films.
28034827	9	25	theme	films	995:999	arg1	flexibility					970:980	the flexibility	966:980	the flexibility of the blend films	966:999	However, at 50% starch content, the flexibility of the blend films was still high, with the elongation at break more than 1000% and tensile strength of 9MPa, which was superior to the commonly LDPE package films.
28034827	2	26	dep	composition	241:251	arg1	the					237:239	the	237:239	the	237:239	The effect of the composition and relative humidity (RH) on the structure and properties of the resulting blends were investigated.
28034827	5	27	theme	blend	595:599	arg1	films					601:605	the blend films	591:605	the blend films	591:605	The fracture surface of the blend films exhibited rough surface, suggesting the tough fracture.
28034827	2	28	dep	structure	287:295	arg1	the					283:285	the	283:285	the	283:285	The effect of the composition and relative humidity (RH) on the structure and properties of the resulting blends were investigated.
28034827	7	29	theme	water	764:768	arg1	uptake					770:775	the water uptake	760:775	the water uptake at equilibrium of the resulting blends	760:814	With the increase of RH, the water uptake at equilibrium of the resulting blends increased.
28034827	1	30	theme	different	166:174	arg1	compositions					176:187	different compositions	166:187	different compositions	166:187	In this work, starch/polyvinyl alcohol (PVA) blend films with different compositions were prepared by melt processing.
28034827	7	31	theme	RH	756:757	arg1	increase					744:751	the increase	740:751	the increase of RH	740:757	With the increase of RH, the water uptake at equilibrium of the resulting blends increased.
28034827	11	32	theme	packaging	1396:1404	arg1	materials					1406:1414	packaging materials	1396:1414	packaging materials	1396:1414	Therefore, these kinds of blend films exhibited wide application potentials as packaging materials.
28034827	11	32	theme	packaging	1396:1404	arg1	potentials					1382:1391	wide application potentials	1365:1391	wide application potentials	1365:1391	Therefore, these kinds of blend films exhibited wide application potentials as packaging materials.
28034827	10	33	theme	Young	1172:1176	arg1	modulus					1180:1186	Young's modulus	1172:1186	Young's modulus	1172:1186	The tensile strength and Young's modulus decreased with the increase of RH, while the elongation at break was enhanced dramatically, indicating the improved flexibility.
28034827	2	34	from	effect	227:232	arg1	properties					301:310	properties	301:310	properties	301:310	The effect of the composition and relative humidity (RH) on the structure and properties of the resulting blends were investigated.
28034827	2	34	from	effect	227:232	arg1	structure					287:295	structure	287:295	structure	287:295	The effect of the composition and relative humidity (RH) on the structure and properties of the resulting blends were investigated.
28034827	8	35	theme	Young	873:877	arg1	modulus					881:887	Young's modulus	873:887	Young's modulus	873:887	The tensile strength, elongation at break and Young's modulus decreased with increasing content of starch.
28034827	11	36	theme	films	1349:1353	arg1	kinds					1334:1338	these kinds	1328:1338	these kinds of blend films	1328:1353	Therefore, these kinds of blend films exhibited wide application potentials as packaging materials.
28034827	9	37	from	content	957:963	arg1	high					1011:1014	high	1011:1014	high	1011:1014	However, at 50% starch content, the flexibility of the blend films was still high, with the elongation at break more than 1000% and tensile strength of 9MPa, which was superior to the commonly LDPE package films.
28034827	9	37	from	content	957:963	arg1	flexibility					970:980	the flexibility	966:980	the flexibility of the blend films	966:999	However, at 50% starch content, the flexibility of the blend films was still high, with the elongation at break more than 1000% and tensile strength of 9MPa, which was superior to the commonly LDPE package films.
28034827	9	38	with	high	1011:1014	arg1	elongation					1026:1035	the elongation	1022:1035	the elongation at break more than 1000% and tensile strength of 9MPa, which was superior to the commonly LDPE package films	1022:1144	However, at 50% starch content, the flexibility of the blend films was still high, with the elongation at break more than 1000% and tensile strength of 9MPa, which was superior to the commonly LDPE package films.
28034827	9	39	dep	break	1040:1044	arg1	%					1060:1060	more than 1000%	1046:1060	break more than 1000%	1040:1060	However, at 50% starch content, the flexibility of the blend films was still high, with the elongation at break more than 1000% and tensile strength of 9MPa, which was superior to the commonly LDPE package films.
28034827	6	40	from	equilibrium	712:722	arg1	uptake					702:707	the water uptake	692:707	the water uptake at equilibrium	692:722	With the increase of starch, the water uptake at equilibrium decreased.
28034827	0	41	theme	alcohol/starch	40:53	arg1	films					61:65	polyvinyl alcohol/starch blend films	30:65	polyvinyl alcohol/starch blend films	30:65	Fabrication and properties of polyvinyl alcohol/starch blend films: Effect of composition and humidity.
28034827	10	42	theme	tensile	1151:1157	arg1	strength					1159:1166	The tensile strength	1147:1166	The tensile strength	1147:1166	The tensile strength and Young's modulus decreased with the increase of RH, while the elongation at break was enhanced dramatically, indicating the improved flexibility.
28034827	3	43	theme	hydrogen	390:397	arg1	interactions					407:418	hydrogen bonding interactions	390:418	hydrogen bonding interactions	390:418	OH groups on starch and PVA formed hydrogen bonding interactions, which could improve the compatibility of the two components.
28034827	0	44	theme	polyvinyl	30:38	arg1	films					61:65	polyvinyl alcohol/starch blend films	30:65	polyvinyl alcohol/starch blend films	30:65	Fabrication and properties of polyvinyl alcohol/starch blend films: Effect of composition and humidity.
28034827	8	45	theme	at	860:861	arg1	break					863:867	elongation at break	849:867	elongation at break	849:867	The tensile strength, elongation at break and Young's modulus decreased with increasing content of starch.
28034827	0	46	theme	films	61:65	arg1	properties					16:25	properties	16:25	properties	16:25	Fabrication and properties of polyvinyl alcohol/starch blend films: Effect of composition and humidity.
28034827	0	46	theme	films	61:65	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication	0:10	Fabrication and properties of polyvinyl alcohol/starch blend films: Effect of composition and humidity.
28034827	3	47	theme	bonding	399:405	arg1	interactions					407:418	hydrogen bonding interactions	390:418	hydrogen bonding interactions	390:418	OH groups on starch and PVA formed hydrogen bonding interactions, which could improve the compatibility of the two components.
28034827	8	48	theme	elongation	849:858	arg1	break					863:867	elongation at break	849:867	elongation at break	849:867	The tensile strength, elongation at break and Young's modulus decreased with increasing content of starch.
28034827	0	49	theme	blend	55:59	arg1	films					61:65	polyvinyl alcohol/starch blend films	30:65	polyvinyl alcohol/starch blend films	30:65	Fabrication and properties of polyvinyl alcohol/starch blend films: Effect of composition and humidity.
28034827	4	50	theme	starch	503:508	arg1	increase					491:498	the increase	487:498	the increase of starch	487:508	With the increase of starch, the degree of crystallinity of PVA component decreased.
28034827	8	51	theme	tensile	831:837	arg1	strength					839:846	The tensile strength	827:846	The tensile strength	827:846	The tensile strength, elongation at break and Young's modulus decreased with increasing content of starch.
28034827	6	52	theme	water	696:700	arg1	uptake					702:707	the water uptake	692:707	the water uptake at equilibrium	692:722	With the increase of starch, the water uptake at equilibrium decreased.
28034827	11	53	theme	blend	1343:1347	arg1	films					1349:1353	blend films	1343:1353	blend films	1343:1353	Therefore, these kinds of blend films exhibited wide application potentials as packaging materials.
28034827	9	54	theme	LDPE	1127:1130	arg1	films					1140:1144	the commonly LDPE package films	1114:1144	the commonly LDPE package films	1114:1144	However, at 50% starch content, the flexibility of the blend films was still high, with the elongation at break more than 1000% and tensile strength of 9MPa, which was superior to the commonly LDPE package films.
28034827	2	55	theme	composition	241:251	arg1	effect					227:232	The effect	223:232	The effect of the composition and relative humidity (RH) on the structure and properties of the resulting blends	223:334	The effect of the composition and relative humidity (RH) on the structure and properties of the resulting blends were investigated.
28034827	9	56	from	strength	1074:1081	arg1	elongation					1026:1035	the elongation	1022:1035	the elongation at break more than 1000% and tensile strength of 9MPa, which was superior to the commonly LDPE package films	1022:1144	However, at 50% starch content, the flexibility of the blend films was still high, with the elongation at break more than 1000% and tensile strength of 9MPa, which was superior to the commonly LDPE package films.
28034827	10	57	from	break	1247:1251	arg1	elongation					1233:1242	the elongation	1229:1242	the elongation at break	1229:1251	The tensile strength and Young's modulus decreased with the increase of RH, while the elongation at break was enhanced dramatically, indicating the improved flexibility.
28034827	9	58	theme	50	946:947	arg1	%					948:948	%	948:948	%	948:948	However, at 50% starch content, the flexibility of the blend films was still high, with the elongation at break more than 1000% and tensile strength of 9MPa, which was superior to the commonly LDPE package films.
28034827	1	59	theme	melt	206:209	arg1	processing					211:220	melt processing	206:220	melt processing	206:220	In this work, starch/polyvinyl alcohol (PVA) blend films with different compositions were prepared by melt processing.
28034827	1	60	with	films	155:159	arg1	compositions					176:187	different compositions	166:187	different compositions	166:187	In this work, starch/polyvinyl alcohol (PVA) blend films with different compositions were prepared by melt processing.
28034827	9	61	theme	package	1132:1138	arg1	films					1140:1144	the commonly LDPE package films	1114:1144	the commonly LDPE package films	1114:1144	However, at 50% starch content, the flexibility of the blend films was still high, with the elongation at break more than 1000% and tensile strength of 9MPa, which was superior to the commonly LDPE package films.
28034827	7	62	from	equilibrium	780:790	arg1	uptake					770:775	the water uptake	760:775	the water uptake at equilibrium of the resulting blends	760:814	With the increase of RH, the water uptake at equilibrium of the resulting blends increased.
28034827	1	63	theme	starch/polyvinyl	118:133	arg1	PVA					144:146	PVA	144:146	PVA	144:146	In this work, starch/polyvinyl alcohol (PVA) blend films with different compositions were prepared by melt processing.
28034827	1	63	theme	starch/polyvinyl	118:133	arg1	alcohol					135:141	starch/polyvinyl alcohol	118:141	starch/polyvinyl alcohol (PVA) blend films with different compositions	118:187	In this work, starch/polyvinyl alcohol (PVA) blend films with different compositions were prepared by melt processing.
28034827	9	64	theme	%	948:948	arg1	content					957:963	50% starch content	946:963	50% starch content	946:963	However, at 50% starch content, the flexibility of the blend films was still high, with the elongation at break more than 1000% and tensile strength of 9MPa, which was superior to the commonly LDPE package films.
28034827	11	65	theme	wide	1365:1368	arg1	materials					1406:1414	packaging materials	1396:1414	packaging materials	1396:1414	Therefore, these kinds of blend films exhibited wide application potentials as packaging materials.
28034827	11	65	theme	wide	1365:1368	arg1	potentials					1382:1391	wide application potentials	1365:1391	wide application potentials	1365:1391	Therefore, these kinds of blend films exhibited wide application potentials as packaging materials.
28034827	6	66	theme	starch	684:689	arg1	increase					672:679	the increase	668:679	the increase of starch	668:689	With the increase of starch, the water uptake at equilibrium decreased.
28034827	1	67	theme	alcohol	135:141	arg1	films					155:159	starch/polyvinyl alcohol (PVA) blend films	118:159	starch/polyvinyl alcohol (PVA) blend films with different compositions	118:187	In this work, starch/polyvinyl alcohol (PVA) blend films with different compositions were prepared by melt processing.
28034827	9	68	theme	starch	950:955	arg1	content					957:963	50% starch content	946:963	50% starch content	946:963	However, at 50% starch content, the flexibility of the blend films was still high, with the elongation at break more than 1000% and tensile strength of 9MPa, which was superior to the commonly LDPE package films.
28034827	0	69	dep	Fabrication	0:10	arg1	Effect					68:73	Effect	68:73	Fabrication and properties of polyvinyl alcohol/starch blend films: Effect of composition and humidity.	0:102	Fabrication and properties of polyvinyl alcohol/starch blend films: Effect of composition and humidity.
28034827	10	70	theme	RH	1219:1220	arg1	increase					1207:1214	the increase	1203:1214	the increase of RH	1203:1220	The tensile strength and Young's modulus decreased with the increase of RH, while the elongation at break was enhanced dramatically, indicating the improved flexibility.
28034827	2	71	theme	blends	329:334	arg1	properties					301:310	properties	301:310	properties	301:310	The effect of the composition and relative humidity (RH) on the structure and properties of the resulting blends were investigated.
28034827	2	71	theme	blends	329:334	arg1	structure					287:295	structure	287:295	structure	287:295	The effect of the composition and relative humidity (RH) on the structure and properties of the resulting blends were investigated.
28034827	11	72	theme	application	1370:1380	arg1	materials					1406:1414	packaging materials	1396:1414	packaging materials	1396:1414	Therefore, these kinds of blend films exhibited wide application potentials as packaging materials.
28034827	11	72	theme	application	1370:1380	arg1	potentials					1382:1391	wide application potentials	1365:1391	wide application potentials	1365:1391	Therefore, these kinds of blend films exhibited wide application potentials as packaging materials.
28925553	4	0	theme	2-deoxy-d-glucose	684:700	arg1	addition					624:631	the addition	620:631	the addition of mannose and the nonmetabolizable mannose analog 2-deoxy-d-glucose	620:700	Based on these results, we found that the addition of mannose and the nonmetabolizable mannose analog 2-deoxy-d-glucose inhibits the biofilm formation of D. vulgaris as well as that of D. desulfuricans; both compounds also dispersed the SRB biofilms.
28925553	0	1	from	Dispersal	0:8	arg1	biofilm					98:104	the biofilm	94:104	the biofilm of Desulfovibrio vulgaris	94:130	Dispersal and inhibitory roles of mannose, 2-deoxy-d-glucose and N-acetylgalactosaminidase on the biofilm of Desulfovibrio vulgaris.
28925553	4	2	dep	D.	736:737	arg1	vulgaris					739:746	D. vulgaris	736:746	D. vulgaris	736:746	Based on these results, we found that the addition of mannose and the nonmetabolizable mannose analog 2-deoxy-d-glucose inhibits the biofilm formation of D. vulgaris as well as that of D. desulfuricans; both compounds also dispersed the SRB biofilms.
28925553	0	3	theme	vulgaris	123:130	arg1	biofilm					98:104	the biofilm	94:104	the biofilm of Desulfovibrio vulgaris	94:130	Dispersal and inhibitory roles of mannose, 2-deoxy-d-glucose and N-acetylgalactosaminidase on the biofilm of Desulfovibrio vulgaris.
28925553	4	4	theme	biofilm	715:721	arg1	formation					723:731	the biofilm formation	711:731	the biofilm formation of D. vulgaris as well as that of D. desulfuricans	711:782	Based on these results, we found that the addition of mannose and the nonmetabolizable mannose analog 2-deoxy-d-glucose inhibits the biofilm formation of D. vulgaris as well as that of D. desulfuricans; both compounds also dispersed the SRB biofilms.
28925553	4	5	theme	D.	767:768	arg1	desulfuricans					770:782	D. desulfuricans	767:782	D. desulfuricans	767:782	Based on these results, we found that the addition of mannose and the nonmetabolizable mannose analog 2-deoxy-d-glucose inhibits the biofilm formation of D. vulgaris as well as that of D. desulfuricans; both compounds also dispersed the SRB biofilms.
28925553	5	6	theme	enzyme	850:855	arg1	effective					911:919	effective	911:919	effective	911:919	In addition, the enzyme N-acetylgalactosaminidase, which degrades GalNAc, was effective in dispersing D. vulgaris biofilms.
28925553	5	6	theme	enzyme	850:855	arg1	N-acetylgalactosaminidase					857:881	the enzyme N-acetylgalactosaminidase	846:881	the enzyme N-acetylgalactosaminidase	846:881	In addition, the enzyme N-acetylgalactosaminidase, which degrades GalNAc, was effective in dispersing D. vulgaris biofilms.
28925553	0	7	theme	Desulfovibrio	109:121	arg1	vulgaris					123:130	Desulfovibrio vulgaris	109:130	Desulfovibrio vulgaris	109:130	Dispersal and inhibitory roles of mannose, 2-deoxy-d-glucose and N-acetylgalactosaminidase on the biofilm of Desulfovibrio vulgaris.
28925553	5	8	from	effective	911:919	arg1	addition					836:843	addition	836:843	addition	836:843	In addition, the enzyme N-acetylgalactosaminidase, which degrades GalNAc, was effective in dispersing D. vulgaris biofilms.
28925553	3	9	dep	components	491:500	arg1	GalNAc					545:550	GalNAc	545:550	GalNAc	545:550	In this study, by utilizing lectin staining, we identified that the biofilm of D. vulgaris also consists of the matrix components mannose, fucose and N-acetylgalactosamine (GalNAc), with mannose predominating.
28925553	3	9	dep	components	491:500	arg1	N-acetylgalactosamine					522:542	N-acetylgalactosamine	522:542	N-acetylgalactosamine (GalNAc)	522:551	In this study, by utilizing lectin staining, we identified that the biofilm of D. vulgaris also consists of the matrix components mannose, fucose and N-acetylgalactosamine (GalNAc), with mannose predominating.
28925553	3	9	dep	components	491:500	arg1	components					491:500	the matrix components mannose, fucose and N-acetylgalactosamine (GalNAc)	480:551	the matrix components mannose, fucose and N-acetylgalactosamine (GalNAc)	480:551	In this study, by utilizing lectin staining, we identified that the biofilm of D. vulgaris also consists of the matrix components mannose, fucose and N-acetylgalactosamine (GalNAc), with mannose predominating.
28925553	3	9	dep	components	491:500	arg1	fucose					511:516	fucose	511:516	fucose	511:516	In this study, by utilizing lectin staining, we identified that the biofilm of D. vulgaris also consists of the matrix components mannose, fucose and N-acetylgalactosamine (GalNAc), with mannose predominating.
28925553	3	9	dep	components	491:500	arg1	mannose					502:508	mannose	502:508	mannose	502:508	In this study, by utilizing lectin staining, we identified that the biofilm of D. vulgaris also consists of the matrix components mannose, fucose and N-acetylgalactosamine (GalNAc), with mannose predominating.
28925553	4	10	theme	mannose	636:642	arg1	addition					624:631	the addition	620:631	the addition of mannose and the nonmetabolizable mannose analog 2-deoxy-d-glucose	620:700	Based on these results, we found that the addition of mannose and the nonmetabolizable mannose analog 2-deoxy-d-glucose inhibits the biofilm formation of D. vulgaris as well as that of D. desulfuricans; both compounds also dispersed the SRB biofilms.
28925553	3	11	theme	mannose	559:565	arg1	predominating					567:579	mannose predominating	559:579	mannose predominating	559:579	In this study, by utilizing lectin staining, we identified that the biofilm of D. vulgaris also consists of the matrix components mannose, fucose and N-acetylgalactosamine (GalNAc), with mannose predominating.
28925553	4	12	theme	nonmetabolizable	652:667	arg1	2-deoxy-d-glucose					684:700	the nonmetabolizable mannose analog 2-deoxy-d-glucose	648:700	the nonmetabolizable mannose analog 2-deoxy-d-glucose	648:700	Based on these results, we found that the addition of mannose and the nonmetabolizable mannose analog 2-deoxy-d-glucose inhibits the biofilm formation of D. vulgaris as well as that of D. desulfuricans; both compounds also dispersed the SRB biofilms.
28925553	3	13	theme	lectin	400:405	arg1	staining					407:414	lectin staining	400:414	lectin staining	400:414	In this study, by utilizing lectin staining, we identified that the biofilm of D. vulgaris also consists of the matrix components mannose, fucose and N-acetylgalactosamine (GalNAc), with mannose predominating.
28925553	4	14	theme	D.	736:737	arg1	formation					723:731	the biofilm formation	711:731	the biofilm formation of D. vulgaris as well as that of D. desulfuricans	711:782	Based on these results, we found that the addition of mannose and the nonmetabolizable mannose analog 2-deoxy-d-glucose inhibits the biofilm formation of D. vulgaris as well as that of D. desulfuricans; both compounds also dispersed the SRB biofilms.
28925553	1	15	theme	major	191:195	arg1	Biofilms					133:140	Biofilms	133:140	Biofilms of sulfate-reducing bacteria (SRB)	133:175	Biofilms of sulfate-reducing bacteria (SRB) are often the major cause of microbiologically influenced corrosion.
28925553	1	15	theme	major	191:195	arg1	cause					197:201	the major cause	187:201	the major cause of microbiologically influenced corrosion	187:243	Biofilms of sulfate-reducing bacteria (SRB) are often the major cause of microbiologically influenced corrosion.
28925553	5	16	theme	vulgaris	938:945	arg1	biofilms					947:954	D. vulgaris biofilms	935:954	D. vulgaris biofilms	935:954	In addition, the enzyme N-acetylgalactosaminidase, which degrades GalNAc, was effective in dispersing D. vulgaris biofilms.
28925553	0	17	theme	inhibitory	14:23	arg1	roles					25:29	inhibitory roles	14:29	inhibitory roles	14:29	Dispersal and inhibitory roles of mannose, 2-deoxy-d-glucose and N-acetylgalactosaminidase on the biofilm of Desulfovibrio vulgaris.
28925553	5	18	theme	D.	935:936	arg1	biofilms					947:954	D. vulgaris biofilms	935:954	D. vulgaris biofilms	935:954	In addition, the enzyme N-acetylgalactosaminidase, which degrades GalNAc, was effective in dispersing D. vulgaris biofilms.
28925553	4	19	theme	analog	677:682	arg1	2-deoxy-d-glucose					684:700	the nonmetabolizable mannose analog 2-deoxy-d-glucose	648:700	the nonmetabolizable mannose analog 2-deoxy-d-glucose	648:700	Based on these results, we found that the addition of mannose and the nonmetabolizable mannose analog 2-deoxy-d-glucose inhibits the biofilm formation of D. vulgaris as well as that of D. desulfuricans; both compounds also dispersed the SRB biofilms.
28925553	0	20	theme	mannose	34:40	arg1	Dispersal					0:8	Dispersal	0:8	Dispersal	0:8	Dispersal and inhibitory roles of mannose, 2-deoxy-d-glucose and N-acetylgalactosaminidase on the biofilm of Desulfovibrio vulgaris.
28925553	0	20	theme	mannose	34:40	arg1	roles					25:29	inhibitory roles	14:29	inhibitory roles	14:29	Dispersal and inhibitory roles of mannose, 2-deoxy-d-glucose and N-acetylgalactosaminidase on the biofilm of Desulfovibrio vulgaris.
28925553	6	21	theme	control	1033:1039	arg1	methods					1041:1047	effective biofilm control methods	1015:1047	effective biofilm control methods	1015:1047	Therefore, by determining composition of the SRB biofilm, effective biofilm control methods may be devised.
28925553	2	22	theme	representative	250:263	arg1	vulgaris					283:290	The representative SRB Desulfovibrio vulgaris	246:290	The representative SRB Desulfovibrio vulgaris	246:290	The representative SRB Desulfovibrio vulgaris has previously been shown to have a biofilm that consists primarily of protein.
28925553	3	23	theme	D.	451:452	arg1	biofilm					440:446	the biofilm	436:446	the biofilm of D. vulgaris	436:461	In this study, by utilizing lectin staining, we identified that the biofilm of D. vulgaris also consists of the matrix components mannose, fucose and N-acetylgalactosamine (GalNAc), with mannose predominating.
28925553	6	24	theme	effective	1015:1023	arg1	methods					1041:1047	effective biofilm control methods	1015:1047	effective biofilm control methods	1015:1047	Therefore, by determining composition of the SRB biofilm, effective biofilm control methods may be devised.
28925553	2	25	contain	have	321:324	arg2	biofilm					328:334	a biofilm	326:334	a biofilm that consists primarily of protein	326:369	The representative SRB Desulfovibrio vulgaris has previously been shown to have a biofilm that consists primarily of protein.
28925553	2	25	contain	have	321:324	arg1	vulgaris					283:290	The representative SRB Desulfovibrio vulgaris	246:290	The representative SRB Desulfovibrio vulgaris	246:290	The representative SRB Desulfovibrio vulgaris has previously been shown to have a biofilm that consists primarily of protein.
28925553	1	26	theme	influenced	224:233	arg1	corrosion					235:243	microbiologically influenced corrosion	206:243	microbiologically influenced corrosion	206:243	Biofilms of sulfate-reducing bacteria (SRB) are often the major cause of microbiologically influenced corrosion.
28925553	0	27	theme	2-deoxy-d-glucose	43:59	arg1	Dispersal					0:8	Dispersal	0:8	Dispersal	0:8	Dispersal and inhibitory roles of mannose, 2-deoxy-d-glucose and N-acetylgalactosaminidase on the biofilm of Desulfovibrio vulgaris.
28925553	0	27	theme	2-deoxy-d-glucose	43:59	arg1	roles					25:29	inhibitory roles	14:29	inhibitory roles	14:29	Dispersal and inhibitory roles of mannose, 2-deoxy-d-glucose and N-acetylgalactosaminidase on the biofilm of Desulfovibrio vulgaris.
28925553	4	28	theme	SRB	819:821	arg1	biofilms					823:830	the SRB biofilms	815:830	the SRB biofilms	815:830	Based on these results, we found that the addition of mannose and the nonmetabolizable mannose analog 2-deoxy-d-glucose inhibits the biofilm formation of D. vulgaris as well as that of D. desulfuricans; both compounds also dispersed the SRB biofilms.
28925553	5	29	from	addition	836:843	arg1	effective					911:919	effective	911:919	effective	911:919	In addition, the enzyme N-acetylgalactosaminidase, which degrades GalNAc, was effective in dispersing D. vulgaris biofilms.
28925553	5	29	from	addition	836:843	arg1	N-acetylgalactosaminidase					857:881	the enzyme N-acetylgalactosaminidase	846:881	the enzyme N-acetylgalactosaminidase	846:881	In addition, the enzyme N-acetylgalactosaminidase, which degrades GalNAc, was effective in dispersing D. vulgaris biofilms.
28925553	3	30	theme	matrix	484:489	arg1	N-acetylgalactosamine					522:542	N-acetylgalactosamine	522:542	N-acetylgalactosamine (GalNAc)	522:551	In this study, by utilizing lectin staining, we identified that the biofilm of D. vulgaris also consists of the matrix components mannose, fucose and N-acetylgalactosamine (GalNAc), with mannose predominating.
28925553	3	30	theme	matrix	484:489	arg1	components					491:500	the matrix components mannose, fucose and N-acetylgalactosamine (GalNAc)	480:551	the matrix components mannose, fucose and N-acetylgalactosamine (GalNAc)	480:551	In this study, by utilizing lectin staining, we identified that the biofilm of D. vulgaris also consists of the matrix components mannose, fucose and N-acetylgalactosamine (GalNAc), with mannose predominating.
28925553	3	30	theme	matrix	484:489	arg1	fucose					511:516	fucose	511:516	fucose	511:516	In this study, by utilizing lectin staining, we identified that the biofilm of D. vulgaris also consists of the matrix components mannose, fucose and N-acetylgalactosamine (GalNAc), with mannose predominating.
28925553	3	30	theme	matrix	484:489	arg1	mannose					502:508	mannose	502:508	mannose	502:508	In this study, by utilizing lectin staining, we identified that the biofilm of D. vulgaris also consists of the matrix components mannose, fucose and N-acetylgalactosamine (GalNAc), with mannose predominating.
28925553	3	31	dep	D.	451:452	arg1	vulgaris					454:461	D. vulgaris	451:461	D. vulgaris	451:461	In this study, by utilizing lectin staining, we identified that the biofilm of D. vulgaris also consists of the matrix components mannose, fucose and N-acetylgalactosamine (GalNAc), with mannose predominating.
28925553	1	32	theme	corrosion	235:243	arg1	Biofilms					133:140	Biofilms	133:140	Biofilms of sulfate-reducing bacteria (SRB)	133:175	Biofilms of sulfate-reducing bacteria (SRB) are often the major cause of microbiologically influenced corrosion.
28925553	1	32	theme	corrosion	235:243	arg1	cause					197:201	the major cause	187:201	the major cause of microbiologically influenced corrosion	187:243	Biofilms of sulfate-reducing bacteria (SRB) are often the major cause of microbiologically influenced corrosion.
28925553	1	33	theme	sulfate-reducing	145:160	arg1	SRB					172:174	SRB	172:174	SRB	172:174	Biofilms of sulfate-reducing bacteria (SRB) are often the major cause of microbiologically influenced corrosion.
28925553	1	33	theme	sulfate-reducing	145:160	arg1	bacteria					162:169	sulfate-reducing bacteria	145:169	sulfate-reducing bacteria (SRB)	145:175	Biofilms of sulfate-reducing bacteria (SRB) are often the major cause of microbiologically influenced corrosion.
28925553	0	34	theme	N-acetylgalactosaminidase	65:89	arg1	Dispersal					0:8	Dispersal	0:8	Dispersal	0:8	Dispersal and inhibitory roles of mannose, 2-deoxy-d-glucose and N-acetylgalactosaminidase on the biofilm of Desulfovibrio vulgaris.
28925553	0	34	theme	N-acetylgalactosaminidase	65:89	arg1	roles					25:29	inhibitory roles	14:29	inhibitory roles	14:29	Dispersal and inhibitory roles of mannose, 2-deoxy-d-glucose and N-acetylgalactosaminidase on the biofilm of Desulfovibrio vulgaris.
28925553	6	35	theme	biofilm	1025:1031	arg1	methods					1041:1047	effective biofilm control methods	1015:1047	effective biofilm control methods	1015:1047	Therefore, by determining composition of the SRB biofilm, effective biofilm control methods may be devised.
28925553	2	36	theme	Desulfovibrio	269:281	arg1	vulgaris					283:290	The representative SRB Desulfovibrio vulgaris	246:290	The representative SRB Desulfovibrio vulgaris	246:290	The representative SRB Desulfovibrio vulgaris has previously been shown to have a biofilm that consists primarily of protein.
28925553	1	37	theme	bacteria	162:169	arg1	Biofilms					133:140	Biofilms	133:140	Biofilms of sulfate-reducing bacteria (SRB)	133:175	Biofilms of sulfate-reducing bacteria (SRB) are often the major cause of microbiologically influenced corrosion.
28925553	1	37	theme	bacteria	162:169	arg1	cause					197:201	the major cause	187:201	the major cause of microbiologically influenced corrosion	187:243	Biofilms of sulfate-reducing bacteria (SRB) are often the major cause of microbiologically influenced corrosion.
28925553	6	38	theme	biofilm	1006:1012	arg1	composition					983:993	composition	983:993	composition of the SRB biofilm	983:1012	Therefore, by determining composition of the SRB biofilm, effective biofilm control methods may be devised.
28925553	0	39	from	roles	25:29	arg1	biofilm					98:104	the biofilm	94:104	the biofilm of Desulfovibrio vulgaris	94:130	Dispersal and inhibitory roles of mannose, 2-deoxy-d-glucose and N-acetylgalactosaminidase on the biofilm of Desulfovibrio vulgaris.
28925553	2	40	theme	SRB	265:267	arg1	vulgaris					283:290	The representative SRB Desulfovibrio vulgaris	246:290	The representative SRB Desulfovibrio vulgaris	246:290	The representative SRB Desulfovibrio vulgaris has previously been shown to have a biofilm that consists primarily of protein.
28925553	4	41	theme	mannose	669:675	arg1	2-deoxy-d-glucose					684:700	the nonmetabolizable mannose analog 2-deoxy-d-glucose	648:700	the nonmetabolizable mannose analog 2-deoxy-d-glucose	648:700	Based on these results, we found that the addition of mannose and the nonmetabolizable mannose analog 2-deoxy-d-glucose inhibits the biofilm formation of D. vulgaris as well as that of D. desulfuricans; both compounds also dispersed the SRB biofilms.
28925553	6	42	theme	SRB	1002:1004	arg1	biofilm					1006:1012	the SRB biofilm	998:1012	the SRB biofilm	998:1012	Therefore, by determining composition of the SRB biofilm, effective biofilm control methods may be devised.
24346765	6	0	theme	glucose	875:881	arg1	comparable					927:936	comparable	927:936	comparable	927:936	The glucose yield was 0.52 g glucose/g for NPRS and was comparable to the yields of 0.50 g glucose/g for PRS and 0.58 g glucose/g for PRSAH.
24346765	6	0	theme	glucose	875:881	arg1	glucose/g					900:908	0.52 g glucose/g	893:908	0.52 g glucose/g for NPRS	893:917	The glucose yield was 0.52 g glucose/g for NPRS and was comparable to the yields of 0.50 g glucose/g for PRS and 0.58 g glucose/g for PRSAH.
24346765	6	0	theme	glucose	875:881	arg1	yield					883:887	The glucose yield	871:887	The glucose yield	871:887	The glucose yield was 0.52 g glucose/g for NPRS and was comparable to the yields of 0.50 g glucose/g for PRS and 0.58 g glucose/g for PRSAH.
24346765	8	1	theme	rice	1258:1261	arg1	straw					1263:1267	ground rice straw	1251:1267	ground rice straw	1251:1267	Overall, the production of fermentable sugars from ground rice straw will be more cost effective if the straw is not pretreated with chemicals.
24346765	5	2	theme	hydrolysis	851:860	arg1	product					862:868	the major hydrolysis product	841:868	the major hydrolysis product	841:868	During enzymatic saccharification, straight aliphatic cellulose was hydrolyzed before branched hemicellulose, and glucose was the major hydrolysis product.
24346765	5	2	theme	hydrolysis	851:860	arg1	glucose					829:835	glucose	829:835	glucose	829:835	During enzymatic saccharification, straight aliphatic cellulose was hydrolyzed before branched hemicellulose, and glucose was the major hydrolysis product.
24346765	8	3	from	straw	1263:1267	arg1	effective					1287:1295	effective	1287:1295	effective	1287:1295	Overall, the production of fermentable sugars from ground rice straw will be more cost effective if the straw is not pretreated with chemicals.
24346765	8	3	from	straw	1263:1267	arg1	production					1213:1222	the production	1209:1222	the production of fermentable sugars from ground rice straw	1209:1267	Overall, the production of fermentable sugars from ground rice straw will be more cost effective if the straw is not pretreated with chemicals.
24346765	8	3	from	straw	1263:1267	arg1	sugars					1239:1244	fermentable sugars	1227:1244	fermentable sugars from ground rice straw	1227:1267	Overall, the production of fermentable sugars from ground rice straw will be more cost effective if the straw is not pretreated with chemicals.
24346765	3	4	theme	pretreated	481:490	arg1	straw					497:501	pretreated rice straw	481:501	pretreated rice straw	481:501	Enzymatic saccharification experiments on non-pretreated rice straw (NPRS), pretreated rice straw (PRS), and pretreated rice straw with acid hydrolysate (PRSAH) were conducted in a series of batch reactors.
24346765	8	5	with	pretreated	1317:1326	arg1	chemicals					1333:1341	chemicals	1333:1341	chemicals	1333:1341	Overall, the production of fermentable sugars from ground rice straw will be more cost effective if the straw is not pretreated with chemicals.
24346765	7	6	theme	rate	1110:1113	arg1	constant					1115:1122	a rate constant	1108:1122	a rate constant of 0.0550 d(-1) for NPRS, 0.0653 d(-1) for PRSAH, and 0.0654 d(-1) for PRS	1108:1197	The hydrolysis of rice straw to produce glucose can be described by a first-order reaction with a rate constant of 0.0550 d(-1) for NPRS, 0.0653 d(-1) for PRSAH, and 0.0654 d(-1) for PRS.
24346765	8	7	theme	sugars	1239:1244	arg1	effective					1287:1295	effective	1287:1295	effective	1287:1295	Overall, the production of fermentable sugars from ground rice straw will be more cost effective if the straw is not pretreated with chemicals.
24346765	8	7	theme	sugars	1239:1244	arg1	production					1213:1222	the production	1209:1222	the production of fermentable sugars from ground rice straw	1209:1267	Overall, the production of fermentable sugars from ground rice straw will be more cost effective if the straw is not pretreated with chemicals.
24346765	7	8	theme	rice	1030:1033	arg1	straw					1035:1039	rice straw	1030:1039	rice straw	1030:1039	The hydrolysis of rice straw to produce glucose can be described by a first-order reaction with a rate constant of 0.0550 d(-1) for NPRS, 0.0653 d(-1) for PRSAH, and 0.0654 d(-1) for PRS.
24346765	2	9	theme	1	284:284	arg1	%					286:286	%	286:286	%	286:286	The composition of rice straw pretreated with 1 % sulfuric acid or 1 % sodium hydroxide solution was compared to rice straw with no chemical pretreatment.
24346765	7	10	with	reaction	1094:1101	arg1	constant					1115:1122	a rate constant	1108:1122	a rate constant of 0.0550 d(-1) for NPRS, 0.0653 d(-1) for PRSAH, and 0.0654 d(-1) for PRS	1108:1197	The hydrolysis of rice straw to produce glucose can be described by a first-order reaction with a rate constant of 0.0550 d(-1) for NPRS, 0.0653 d(-1) for PRSAH, and 0.0654 d(-1) for PRS.
24346765	0	11	theme	rice	100:103	arg1	straw					105:109	rice straw	100:109	rice straw	100:109	Study of chemical pretreatment and enzymatic saccharification for producing fermentable sugars from rice straw.
24346765	7	12	theme	straw	1035:1039	arg1	hydrolysis					1016:1025	The hydrolysis	1012:1025	The hydrolysis of rice straw to produce glucose	1012:1058	The hydrolysis of rice straw to produce glucose can be described by a first-order reaction with a rate constant of 0.0550 d(-1) for NPRS, 0.0653 d(-1) for PRSAH, and 0.0654 d(-1) for PRS.
24346765	4	13	theme	cellulose	673:681	arg1	content					683:689	the cellulose content	669:689	the cellulose content	669:689	The results indicated that pretreating the rice straw with dilute acid and base increased the cellulose content from 38 % to over 50 %.
24346765	7	14	theme	d	1157:1157	arg1	constant					1115:1122	a rate constant	1108:1122	a rate constant of 0.0550 d(-1) for NPRS, 0.0653 d(-1) for PRSAH, and 0.0654 d(-1) for PRS	1108:1197	The hydrolysis of rice straw to produce glucose can be described by a first-order reaction with a rate constant of 0.0550 d(-1) for NPRS, 0.0653 d(-1) for PRSAH, and 0.0654 d(-1) for PRS.
24346765	5	15	theme	straight	750:757	arg1	cellulose					769:777	straight aliphatic cellulose	750:777	straight aliphatic cellulose	750:777	During enzymatic saccharification, straight aliphatic cellulose was hydrolyzed before branched hemicellulose, and glucose was the major hydrolysis product.
24346765	4	16	theme	rice	622:625	arg1	straw					627:631	the rice straw	618:631	the rice straw	618:631	The results indicated that pretreating the rice straw with dilute acid and base increased the cellulose content from 38 % to over 50 %.
24346765	3	17	theme	non-pretreated	414:427	arg1	NPRS					441:444	NPRS	441:444	NPRS	441:444	Enzymatic saccharification experiments on non-pretreated rice straw (NPRS), pretreated rice straw (PRS), and pretreated rice straw with acid hydrolysate (PRSAH) were conducted in a series of batch reactors.
24346765	3	17	theme	non-pretreated	414:427	arg1	straw					434:438	non-pretreated rice straw	414:438	non-pretreated rice straw (NPRS)	414:445	Enzymatic saccharification experiments on non-pretreated rice straw (NPRS), pretreated rice straw (PRS), and pretreated rice straw with acid hydrolysate (PRSAH) were conducted in a series of batch reactors.
24346765	2	18	theme	sulfuric	267:274	arg1	acid					276:279	1 % sulfuric acid	263:279	1 % sulfuric acid	263:279	The composition of rice straw pretreated with 1 % sulfuric acid or 1 % sodium hydroxide solution was compared to rice straw with no chemical pretreatment.
24346765	8	19	from	production	1213:1222	arg1	straw					1263:1267	ground rice straw	1251:1267	ground rice straw	1251:1267	Overall, the production of fermentable sugars from ground rice straw will be more cost effective if the straw is not pretreated with chemicals.
24346765	1	20	theme	rice	181:184	arg1	straw					186:190	rice straw	181:190	rice straw	181:190	This study evaluated a cost-effective approach for the conversion of rice straw into fermentable sugars.
24346765	3	21	theme	Enzymatic	372:380	arg1	experiments					399:409	Enzymatic saccharification experiments	372:409	Enzymatic saccharification experiments on non-pretreated rice straw (NPRS), pretreated rice straw (PRS), and pretreated rice straw with acid hydrolysate (PRSAH)	372:531	Enzymatic saccharification experiments on non-pretreated rice straw (NPRS), pretreated rice straw (PRS), and pretreated rice straw with acid hydrolysate (PRSAH) were conducted in a series of batch reactors.
24346765	7	22	theme	d	1134:1134	arg1	constant					1115:1122	a rate constant	1108:1122	a rate constant of 0.0550 d(-1) for NPRS, 0.0653 d(-1) for PRSAH, and 0.0654 d(-1) for PRS	1108:1197	The hydrolysis of rice straw to produce glucose can be described by a first-order reaction with a rate constant of 0.0550 d(-1) for NPRS, 0.0653 d(-1) for PRSAH, and 0.0654 d(-1) for PRS.
24346765	7	23	theme	first-order	1082:1092	arg1	reaction					1094:1101	a first-order reaction	1080:1101	a first-order reaction with a rate constant of 0.0550 d(-1) for NPRS, 0.0653 d(-1) for PRSAH, and 0.0654 d(-1) for PRS	1080:1197	The hydrolysis of rice straw to produce glucose can be described by a first-order reaction with a rate constant of 0.0550 d(-1) for NPRS, 0.0653 d(-1) for PRSAH, and 0.0654 d(-1) for PRS.
24346765	1	24	theme	straw	186:190	arg1	conversion					167:176	the conversion	163:176	the conversion of rice straw into fermentable sugars	163:214	This study evaluated a cost-effective approach for the conversion of rice straw into fermentable sugars.
24346765	3	25	theme	saccharification	382:397	arg1	experiments					399:409	Enzymatic saccharification experiments	372:409	Enzymatic saccharification experiments on non-pretreated rice straw (NPRS), pretreated rice straw (PRS), and pretreated rice straw with acid hydrolysate (PRSAH)	372:531	Enzymatic saccharification experiments on non-pretreated rice straw (NPRS), pretreated rice straw (PRS), and pretreated rice straw with acid hydrolysate (PRSAH) were conducted in a series of batch reactors.
24346765	3	26	with	experiments	399:409	arg1	hydrolysate					513:523	acid hydrolysate	508:523	acid hydrolysate (PRSAH)	508:531	Enzymatic saccharification experiments on non-pretreated rice straw (NPRS), pretreated rice straw (PRS), and pretreated rice straw with acid hydrolysate (PRSAH) were conducted in a series of batch reactors.
24346765	3	26	with	experiments	399:409	arg1	PRSAH					526:530	PRSAH	526:530	PRSAH	526:530	Enzymatic saccharification experiments on non-pretreated rice straw (NPRS), pretreated rice straw (PRS), and pretreated rice straw with acid hydrolysate (PRSAH) were conducted in a series of batch reactors.
24346765	2	27	theme	hydroxide	295:303	arg1	solution					305:312	1 % sodium hydroxide solution	284:312	1 % sodium hydroxide solution	284:312	The composition of rice straw pretreated with 1 % sulfuric acid or 1 % sodium hydroxide solution was compared to rice straw with no chemical pretreatment.
24346765	6	28	theme	g	989:989	arg1	glucose/g					991:999	0.58 g glucose/g	984:999	0.58 g glucose/g for PRSAH	984:1009	The glucose yield was 0.52 g glucose/g for NPRS and was comparable to the yields of 0.50 g glucose/g for PRS and 0.58 g glucose/g for PRSAH.
24346765	0	29	theme	pretreatment	18:29	arg1	Study					0:4	Study	0:4	Study of chemical pretreatment and enzymatic saccharification for producing fermentable sugars from rice straw.	0:110	Study of chemical pretreatment and enzymatic saccharification for producing fermentable sugars from rice straw.
24346765	5	30	theme	aliphatic	759:767	arg1	cellulose					769:777	straight aliphatic cellulose	750:777	straight aliphatic cellulose	750:777	During enzymatic saccharification, straight aliphatic cellulose was hydrolyzed before branched hemicellulose, and glucose was the major hydrolysis product.
24346765	2	31	theme	sodium	288:293	arg1	solution					305:312	1 % sodium hydroxide solution	284:312	1 % sodium hydroxide solution	284:312	The composition of rice straw pretreated with 1 % sulfuric acid or 1 % sodium hydroxide solution was compared to rice straw with no chemical pretreatment.
24346765	1	32	theme	fermentable	197:207	arg1	sugars					209:214	fermentable sugars	197:214	fermentable sugars	197:214	This study evaluated a cost-effective approach for the conversion of rice straw into fermentable sugars.
24346765	0	33	theme	chemical	9:16	arg1	pretreatment					18:29	chemical pretreatment	9:29	chemical pretreatment	9:29	Study of chemical pretreatment and enzymatic saccharification for producing fermentable sugars from rice straw.
24346765	2	34	theme	%	286:286	arg1	solution					305:312	1 % sodium hydroxide solution	284:312	1 % sodium hydroxide solution	284:312	The composition of rice straw pretreated with 1 % sulfuric acid or 1 % sodium hydroxide solution was compared to rice straw with no chemical pretreatment.
24346765	3	35	theme	rice	492:495	arg1	straw					497:501	pretreated rice straw	481:501	pretreated rice straw	481:501	Enzymatic saccharification experiments on non-pretreated rice straw (NPRS), pretreated rice straw (PRS), and pretreated rice straw with acid hydrolysate (PRSAH) were conducted in a series of batch reactors.
24346765	0	36	theme	enzymatic	35:43	arg1	saccharification					45:60	enzymatic saccharification	35:60	enzymatic saccharification	35:60	Study of chemical pretreatment and enzymatic saccharification for producing fermentable sugars from rice straw.
24346765	2	37	theme	straw	241:245	arg1	composition					221:231	The composition	217:231	The composition of rice straw pretreated with 1 % sulfuric acid or 1 % sodium hydroxide solution	217:312	The composition of rice straw pretreated with 1 % sulfuric acid or 1 % sodium hydroxide solution was compared to rice straw with no chemical pretreatment.
24346765	5	38	theme	branched	801:808	arg1	hemicellulose					810:822	branched hemicellulose	801:822	branched hemicellulose	801:822	During enzymatic saccharification, straight aliphatic cellulose was hydrolyzed before branched hemicellulose, and glucose was the major hydrolysis product.
24346765	3	39	theme	rice	429:432	arg1	NPRS					441:444	NPRS	441:444	NPRS	441:444	Enzymatic saccharification experiments on non-pretreated rice straw (NPRS), pretreated rice straw (PRS), and pretreated rice straw with acid hydrolysate (PRSAH) were conducted in a series of batch reactors.
24346765	3	39	theme	rice	429:432	arg1	straw					434:438	non-pretreated rice straw	414:438	non-pretreated rice straw (NPRS)	414:445	Enzymatic saccharification experiments on non-pretreated rice straw (NPRS), pretreated rice straw (PRS), and pretreated rice straw with acid hydrolysate (PRSAH) were conducted in a series of batch reactors.
24346765	5	40	theme	enzymatic	722:730	arg1	saccharification					732:747	enzymatic saccharification	722:747	enzymatic saccharification	722:747	During enzymatic saccharification, straight aliphatic cellulose was hydrolyzed before branched hemicellulose, and glucose was the major hydrolysis product.
24346765	2	41	theme	rice	236:239	arg1	straw					241:245	rice straw	236:245	rice straw pretreated with 1 % sulfuric acid or 1 % sodium hydroxide solution	236:312	The composition of rice straw pretreated with 1 % sulfuric acid or 1 % sodium hydroxide solution was compared to rice straw with no chemical pretreatment.
24346765	3	42	theme	pretreated	448:457	arg1	PRS					471:473	PRS	471:473	PRS	471:473	Enzymatic saccharification experiments on non-pretreated rice straw (NPRS), pretreated rice straw (PRS), and pretreated rice straw with acid hydrolysate (PRSAH) were conducted in a series of batch reactors.
24346765	3	42	theme	pretreated	448:457	arg1	straw					464:468	pretreated rice straw	448:468	pretreated rice straw (PRS)	448:474	Enzymatic saccharification experiments on non-pretreated rice straw (NPRS), pretreated rice straw (PRS), and pretreated rice straw with acid hydrolysate (PRSAH) were conducted in a series of batch reactors.
24346765	6	43	theme	g	898:898	arg1	comparable					927:936	comparable	927:936	comparable	927:936	The glucose yield was 0.52 g glucose/g for NPRS and was comparable to the yields of 0.50 g glucose/g for PRS and 0.58 g glucose/g for PRSAH.
24346765	6	43	theme	g	898:898	arg1	glucose/g					900:908	0.52 g glucose/g	893:908	0.52 g glucose/g for NPRS	893:917	The glucose yield was 0.52 g glucose/g for NPRS and was comparable to the yields of 0.50 g glucose/g for PRS and 0.58 g glucose/g for PRSAH.
24346765	6	43	theme	g	898:898	arg1	yield					883:887	The glucose yield	871:887	The glucose yield	871:887	The glucose yield was 0.52 g glucose/g for NPRS and was comparable to the yields of 0.50 g glucose/g for PRS and 0.58 g glucose/g for PRSAH.
24346765	8	44	theme	fermentable	1227:1237	arg1	sugars					1239:1244	fermentable sugars	1227:1244	fermentable sugars from ground rice straw	1227:1267	Overall, the production of fermentable sugars from ground rice straw will be more cost effective if the straw is not pretreated with chemicals.
24346765	2	45	theme	rice	330:333	arg1	straw					335:339	rice straw	330:339	rice straw	330:339	The composition of rice straw pretreated with 1 % sulfuric acid or 1 % sodium hydroxide solution was compared to rice straw with no chemical pretreatment.
24346765	0	46	theme	saccharification	45:60	arg1	Study					0:4	Study	0:4	Study of chemical pretreatment and enzymatic saccharification for producing fermentable sugars from rice straw.	0:110	Study of chemical pretreatment and enzymatic saccharification for producing fermentable sugars from rice straw.
24346765	8	47	theme	ground	1251:1256	arg1	straw					1263:1267	ground rice straw	1251:1267	ground rice straw	1251:1267	Overall, the production of fermentable sugars from ground rice straw will be more cost effective if the straw is not pretreated with chemicals.
24346765	2	48	theme	%	265:265	arg1	acid					276:279	1 % sulfuric acid	263:279	1 % sulfuric acid	263:279	The composition of rice straw pretreated with 1 % sulfuric acid or 1 % sodium hydroxide solution was compared to rice straw with no chemical pretreatment.
24346765	6	49	theme	glucose/g	962:970	arg1	yields					945:950	the yields	941:950	the yields of 0.50 g glucose/g for PRS and 0.58 g glucose/g for PRSAH	941:1009	The glucose yield was 0.52 g glucose/g for NPRS and was comparable to the yields of 0.50 g glucose/g for PRS and 0.58 g glucose/g for PRSAH.
24346765	3	50	theme	acid	508:511	arg1	hydrolysate					513:523	acid hydrolysate	508:523	acid hydrolysate (PRSAH)	508:531	Enzymatic saccharification experiments on non-pretreated rice straw (NPRS), pretreated rice straw (PRS), and pretreated rice straw with acid hydrolysate (PRSAH) were conducted in a series of batch reactors.
24346765	3	50	theme	acid	508:511	arg1	PRSAH					526:530	PRSAH	526:530	PRSAH	526:530	Enzymatic saccharification experiments on non-pretreated rice straw (NPRS), pretreated rice straw (PRS), and pretreated rice straw with acid hydrolysate (PRSAH) were conducted in a series of batch reactors.
24346765	2	51	theme	1	263:263	arg1	%					265:265	%	265:265	%	265:265	The composition of rice straw pretreated with 1 % sulfuric acid or 1 % sodium hydroxide solution was compared to rice straw with no chemical pretreatment.
24346765	1	52	theme	cost-effective	135:148	arg1	approach					150:157	a cost-effective approach	133:157	a cost-effective approach for the conversion of rice straw into fermentable sugars	133:214	This study evaluated a cost-effective approach for the conversion of rice straw into fermentable sugars.
24346765	6	53	theme	g	960:960	arg1	glucose/g					962:970	0.50 g glucose/g	955:970	0.50 g glucose/g for PRS and 0.58 g glucose/g for PRSAH	955:1009	The glucose yield was 0.52 g glucose/g for NPRS and was comparable to the yields of 0.50 g glucose/g for PRS and 0.58 g glucose/g for PRSAH.
24346765	0	54	theme	fermentable	76:86	arg1	sugars					88:93	fermentable sugars	76:93	fermentable sugars	76:93	Study of chemical pretreatment and enzymatic saccharification for producing fermentable sugars from rice straw.
24346765	5	55	theme	major	845:849	arg1	product					862:868	the major hydrolysis product	841:868	the major hydrolysis product	841:868	During enzymatic saccharification, straight aliphatic cellulose was hydrolyzed before branched hemicellulose, and glucose was the major hydrolysis product.
24346765	5	55	theme	major	845:849	arg1	glucose					829:835	glucose	829:835	glucose	829:835	During enzymatic saccharification, straight aliphatic cellulose was hydrolyzed before branched hemicellulose, and glucose was the major hydrolysis product.
24346765	3	56	theme	batch	563:567	arg1	reactors					569:576	batch reactors	563:576	batch reactors	563:576	Enzymatic saccharification experiments on non-pretreated rice straw (NPRS), pretreated rice straw (PRS), and pretreated rice straw with acid hydrolysate (PRSAH) were conducted in a series of batch reactors.
24346765	3	57	theme	rice	459:462	arg1	PRS					471:473	PRS	471:473	PRS	471:473	Enzymatic saccharification experiments on non-pretreated rice straw (NPRS), pretreated rice straw (PRS), and pretreated rice straw with acid hydrolysate (PRSAH) were conducted in a series of batch reactors.
24346765	3	57	theme	rice	459:462	arg1	straw					464:468	pretreated rice straw	448:468	pretreated rice straw (PRS)	448:474	Enzymatic saccharification experiments on non-pretreated rice straw (NPRS), pretreated rice straw (PRS), and pretreated rice straw with acid hydrolysate (PRSAH) were conducted in a series of batch reactors.
24346765	2	58	theme	chemical	349:356	arg1	pretreatment					358:369	no chemical pretreatment	346:369	no chemical pretreatment	346:369	The composition of rice straw pretreated with 1 % sulfuric acid or 1 % sodium hydroxide solution was compared to rice straw with no chemical pretreatment.
24346765	7	59	theme	d	1185:1185	arg1	constant					1115:1122	a rate constant	1108:1122	a rate constant of 0.0550 d(-1) for NPRS, 0.0653 d(-1) for PRSAH, and 0.0654 d(-1) for PRS	1108:1197	The hydrolysis of rice straw to produce glucose can be described by a first-order reaction with a rate constant of 0.0550 d(-1) for NPRS, 0.0653 d(-1) for PRSAH, and 0.0654 d(-1) for PRS.
24346765	3	60	from	experiments	399:409	arg1	NPRS					441:444	NPRS	441:444	NPRS	441:444	Enzymatic saccharification experiments on non-pretreated rice straw (NPRS), pretreated rice straw (PRS), and pretreated rice straw with acid hydrolysate (PRSAH) were conducted in a series of batch reactors.
24346765	3	60	from	experiments	399:409	arg1	straw					434:438	non-pretreated rice straw	414:438	non-pretreated rice straw (NPRS)	414:445	Enzymatic saccharification experiments on non-pretreated rice straw (NPRS), pretreated rice straw (PRS), and pretreated rice straw with acid hydrolysate (PRSAH) were conducted in a series of batch reactors.
24346765	3	60	from	experiments	399:409	arg1	straw					464:468	pretreated rice straw	448:468	pretreated rice straw (PRS)	448:474	Enzymatic saccharification experiments on non-pretreated rice straw (NPRS), pretreated rice straw (PRS), and pretreated rice straw with acid hydrolysate (PRSAH) were conducted in a series of batch reactors.
24346765	3	60	from	experiments	399:409	arg1	PRS					471:473	PRS	471:473	PRS	471:473	Enzymatic saccharification experiments on non-pretreated rice straw (NPRS), pretreated rice straw (PRS), and pretreated rice straw with acid hydrolysate (PRSAH) were conducted in a series of batch reactors.
24346765	3	60	from	experiments	399:409	arg1	straw					497:501	pretreated rice straw	481:501	pretreated rice straw	481:501	Enzymatic saccharification experiments on non-pretreated rice straw (NPRS), pretreated rice straw (PRS), and pretreated rice straw with acid hydrolysate (PRSAH) were conducted in a series of batch reactors.
24346765	3	61	theme	reactors	569:576	arg1	series					553:558	a series	551:558	a series of batch reactors	551:576	Enzymatic saccharification experiments on non-pretreated rice straw (NPRS), pretreated rice straw (PRS), and pretreated rice straw with acid hydrolysate (PRSAH) were conducted in a series of batch reactors.
24346765	4	62	theme	dilute	638:643	arg1	acid					645:648	dilute acid	638:648	dilute acid	638:648	The results indicated that pretreating the rice straw with dilute acid and base increased the cellulose content from 38 % to over 50 %.
27311675	5	0	theme	wall	1114:1117	arg1	layer					1096:1100	the peptidoglycan layer	1078:1100	the peptidoglycan layer of the cell wall	1078:1117	Thus, it is expected that for gram-negative bacteria only the outer membrane and part of the periplasmic peptidoglycan layer is probed during analysis, and for gram-positive bacteria only the top nanometers of the peptidoglycan layer of the cell wall is monitored.
27311675	3	1	from	changes	707:713	arg1	composition					732:742	the cell wall composition	718:742	the cell wall composition	718:742	The method can be used to monitor changes in the cell wall composition following environmental stimuli or genetic mutations.
27311675	1	2	theme	surface	164:170	arg1	composition					181:191	the average surface chemical composition	152:191	the average surface chemical composition	152:191	This chapter describes a method for measuring the average surface chemical composition with respect to lipids, polysaccharides, and peptides (protein + peptidoglycan) for the outer part of the bacterial cell wall.
27311675	5	3	theme	peptidoglycan	1082:1094	arg1	layer					1096:1100	the peptidoglycan layer	1078:1100	the peptidoglycan layer of the cell wall	1078:1117	Thus, it is expected that for gram-negative bacteria only the outer membrane and part of the periplasmic peptidoglycan layer is probed during analysis, and for gram-positive bacteria only the top nanometers of the peptidoglycan layer of the cell wall is monitored.
27311675	0	4	theme	Photoelectron	78:90	arg1	Spectroscopy					92:103	Cryogenic X-Ray Photoelectron Spectroscopy	62:103	Cryogenic X-Ray Photoelectron Spectroscopy	62:103	Analysis of Bacterial Cell Surface Chemical Composition Using Cryogenic X-Ray Photoelectron Spectroscopy.
27311675	2	5	theme	cryogenic	613:621	arg1	temperatures					623:634	cryogenic temperatures	613:634	cryogenic temperatures (for example using liquid nitrogen)	613:670	Bacterial cultures grown over night are washed with a buffer or saline at controlled pH. The analysis is done on fast-frozen bacterial cell pellets obtained after centrifugation, and the analysis requires access to X-ray photoelectron spectroscopy instrumentation that can perform analyses at cryogenic temperatures (for example using liquid nitrogen).
27311675	1	6	theme	chemical	172:179	arg1	composition					181:191	the average surface chemical composition	152:191	the average surface chemical composition	152:191	This chapter describes a method for measuring the average surface chemical composition with respect to lipids, polysaccharides, and peptides (protein + peptidoglycan) for the outer part of the bacterial cell wall.
27311675	5	7	theme	outer	930:934	arg1	membrane					936:943	only the outer membrane	921:943	only the outer membrane	921:943	Thus, it is expected that for gram-negative bacteria only the outer membrane and part of the periplasmic peptidoglycan layer is probed during analysis, and for gram-positive bacteria only the top nanometers of the peptidoglycan layer of the cell wall is monitored.
27311675	3	8	theme	cell	722:725	arg1	composition					732:742	the cell wall composition	718:742	the cell wall composition	718:742	The method can be used to monitor changes in the cell wall composition following environmental stimuli or genetic mutations.
27311675	1	9	theme	outer	281:285	arg1	part					287:290	the outer part	277:290	the outer part of the bacterial cell wall	277:317	This chapter describes a method for measuring the average surface chemical composition with respect to lipids, polysaccharides, and peptides (protein + peptidoglycan) for the outer part of the bacterial cell wall.
27311675	3	10	theme	wall	727:730	arg1	composition					732:742	the cell wall composition	718:742	the cell wall composition	718:742	The method can be used to monitor changes in the cell wall composition following environmental stimuli or genetic mutations.
27311675	2	11	theme	liquid	655:660	arg1	nitrogen					662:669	liquid nitrogen	655:669	liquid nitrogen	655:669	Bacterial cultures grown over night are washed with a buffer or saline at controlled pH. The analysis is done on fast-frozen bacterial cell pellets obtained after centrifugation, and the analysis requires access to X-ray photoelectron spectroscopy instrumentation that can perform analyses at cryogenic temperatures (for example using liquid nitrogen).
27311675	5	12	theme	periplasmic	961:971	arg1	layer					987:991	the periplasmic peptidoglycan layer	957:991	the periplasmic peptidoglycan layer	957:991	Thus, it is expected that for gram-negative bacteria only the outer membrane and part of the periplasmic peptidoglycan layer is probed during analysis, and for gram-positive bacteria only the top nanometers of the peptidoglycan layer of the cell wall is monitored.
27311675	2	13	theme	bacterial	445:453	arg1	pellets					460:466	fast-frozen bacterial cell pellets	433:466	fast-frozen bacterial cell pellets obtained after centrifugation	433:496	Bacterial cultures grown over night are washed with a buffer or saline at controlled pH. The analysis is done on fast-frozen bacterial cell pellets obtained after centrifugation, and the analysis requires access to X-ray photoelectron spectroscopy instrumentation that can perform analyses at cryogenic temperatures (for example using liquid nitrogen).
27311675	5	14	theme	peptidoglycan	973:985	arg1	layer					987:991	the periplasmic peptidoglycan layer	957:991	the periplasmic peptidoglycan layer	957:991	Thus, it is expected that for gram-negative bacteria only the outer membrane and part of the periplasmic peptidoglycan layer is probed during analysis, and for gram-positive bacteria only the top nanometers of the peptidoglycan layer of the cell wall is monitored.
27311675	2	15	theme	fast-frozen	433:443	arg1	pellets					460:466	fast-frozen bacterial cell pellets	433:466	fast-frozen bacterial cell pellets obtained after centrifugation	433:496	Bacterial cultures grown over night are washed with a buffer or saline at controlled pH. The analysis is done on fast-frozen bacterial cell pellets obtained after centrifugation, and the analysis requires access to X-ray photoelectron spectroscopy instrumentation that can perform analyses at cryogenic temperatures (for example using liquid nitrogen).
27311675	4	16	theme	wall	862:865	arg1	part					845:848	the outermost part	831:848	the outermost part of the cell wall	831:865	The data obtained originate from the outermost part of the cell wall.
27311675	2	17	theme	Bacterial	320:328	arg1	cultures					330:337	Bacterial cultures	320:337	Bacterial cultures grown over night	320:354	Bacterial cultures grown over night are washed with a buffer or saline at controlled pH. The analysis is done on fast-frozen bacterial cell pellets obtained after centrifugation, and the analysis requires access to X-ray photoelectron spectroscopy instrumentation that can perform analyses at cryogenic temperatures (for example using liquid nitrogen).
27311675	5	18	theme	layer	1096:1100	arg1	nanometers					1064:1073	only the top nanometers	1051:1073	only the top nanometers of the peptidoglycan layer of the cell wall	1051:1117	Thus, it is expected that for gram-negative bacteria only the outer membrane and part of the periplasmic peptidoglycan layer is probed during analysis, and for gram-positive bacteria only the top nanometers of the peptidoglycan layer of the cell wall is monitored.
27311675	3	19	theme	environmental	754:766	arg1	stimuli					768:774	environmental stimuli	754:774	environmental stimuli	754:774	The method can be used to monitor changes in the cell wall composition following environmental stimuli or genetic mutations.
27311675	4	20	theme	cell	857:860	arg1	wall					862:865	the cell wall	853:865	the cell wall	853:865	The data obtained originate from the outermost part of the cell wall.
27311675	0	21	theme	Cell	22:25	arg1	Composition					44:54	Bacterial Cell Surface Chemical Composition	12:54	Bacterial Cell Surface Chemical Composition	12:54	Analysis of Bacterial Cell Surface Chemical Composition Using Cryogenic X-Ray Photoelectron Spectroscopy.
27311675	2	22	theme	spectroscopy	555:566	arg1	instrumentation					568:582	X-ray photoelectron spectroscopy instrumentation	535:582	X-ray photoelectron spectroscopy instrumentation that can perform analyses at cryogenic temperatures (for example using liquid nitrogen)	535:670	Bacterial cultures grown over night are washed with a buffer or saline at controlled pH. The analysis is done on fast-frozen bacterial cell pellets obtained after centrifugation, and the analysis requires access to X-ray photoelectron spectroscopy instrumentation that can perform analyses at cryogenic temperatures (for example using liquid nitrogen).
27311675	0	23	theme	Bacterial	12:20	arg1	Composition					44:54	Bacterial Cell Surface Chemical Composition	12:54	Bacterial Cell Surface Chemical Composition	12:54	Analysis of Bacterial Cell Surface Chemical Composition Using Cryogenic X-Ray Photoelectron Spectroscopy.
27311675	2	24	theme	photoelectron	541:553	arg1	spectroscopy					555:566	X-ray photoelectron spectroscopy	535:566	X-ray photoelectron spectroscopy instrumentation that can perform analyses at cryogenic temperatures (for example using liquid nitrogen)	535:670	Bacterial cultures grown over night are washed with a buffer or saline at controlled pH. The analysis is done on fast-frozen bacterial cell pellets obtained after centrifugation, and the analysis requires access to X-ray photoelectron spectroscopy instrumentation that can perform analyses at cryogenic temperatures (for example using liquid nitrogen).
27311675	5	25	theme	top	1060:1062	arg1	nanometers					1064:1073	only the top nanometers	1051:1073	only the top nanometers of the peptidoglycan layer of the cell wall	1051:1117	Thus, it is expected that for gram-negative bacteria only the outer membrane and part of the periplasmic peptidoglycan layer is probed during analysis, and for gram-positive bacteria only the top nanometers of the peptidoglycan layer of the cell wall is monitored.
27311675	0	26	theme	Chemical	35:42	arg1	Composition					44:54	Bacterial Cell Surface Chemical Composition	12:54	Bacterial Cell Surface Chemical Composition	12:54	Analysis of Bacterial Cell Surface Chemical Composition Using Cryogenic X-Ray Photoelectron Spectroscopy.
27311675	1	27	theme	bacterial	299:307	arg1	wall					314:317	the bacterial cell wall	295:317	the bacterial cell wall	295:317	This chapter describes a method for measuring the average surface chemical composition with respect to lipids, polysaccharides, and peptides (protein + peptidoglycan) for the outer part of the bacterial cell wall.
27311675	2	28	theme	X-ray	535:539	arg1	spectroscopy					555:566	X-ray photoelectron spectroscopy	535:566	X-ray photoelectron spectroscopy instrumentation that can perform analyses at cryogenic temperatures (for example using liquid nitrogen)	535:670	Bacterial cultures grown over night are washed with a buffer or saline at controlled pH. The analysis is done on fast-frozen bacterial cell pellets obtained after centrifugation, and the analysis requires access to X-ray photoelectron spectroscopy instrumentation that can perform analyses at cryogenic temperatures (for example using liquid nitrogen).
27311675	2	29	dep	pH.	405:407	arg1	requires					516:523	requires	516:523	requires access to X-ray photoelectron spectroscopy instrumentation that can perform analyses at cryogenic temperatures (for example using liquid nitrogen)	516:670	Bacterial cultures grown over night are washed with a buffer or saline at controlled pH. The analysis is done on fast-frozen bacterial cell pellets obtained after centrifugation, and the analysis requires access to X-ray photoelectron spectroscopy instrumentation that can perform analyses at cryogenic temperatures (for example using liquid nitrogen).
27311675	2	29	dep	pH.	405:407	arg1	done					425:428	done	425:428	is done on fast-frozen bacterial cell pellets obtained after centrifugation	422:496	Bacterial cultures grown over night are washed with a buffer or saline at controlled pH. The analysis is done on fast-frozen bacterial cell pellets obtained after centrifugation, and the analysis requires access to X-ray photoelectron spectroscopy instrumentation that can perform analyses at cryogenic temperatures (for example using liquid nitrogen).
27311675	0	30	theme	Surface	27:33	arg1	Composition					44:54	Bacterial Cell Surface Chemical Composition	12:54	Bacterial Cell Surface Chemical Composition	12:54	Analysis of Bacterial Cell Surface Chemical Composition Using Cryogenic X-Ray Photoelectron Spectroscopy.
27311675	1	31	theme	cell	309:312	arg1	wall					314:317	the bacterial cell wall	295:317	the bacterial cell wall	295:317	This chapter describes a method for measuring the average surface chemical composition with respect to lipids, polysaccharides, and peptides (protein + peptidoglycan) for the outer part of the bacterial cell wall.
27311675	2	32	theme	cell	455:458	arg1	pellets					460:466	fast-frozen bacterial cell pellets	433:466	fast-frozen bacterial cell pellets obtained after centrifugation	433:496	Bacterial cultures grown over night are washed with a buffer or saline at controlled pH. The analysis is done on fast-frozen bacterial cell pellets obtained after centrifugation, and the analysis requires access to X-ray photoelectron spectroscopy instrumentation that can perform analyses at cryogenic temperatures (for example using liquid nitrogen).
27311675	1	33	theme	wall	314:317	arg1	part					287:290	the outer part	277:290	the outer part of the bacterial cell wall	277:317	This chapter describes a method for measuring the average surface chemical composition with respect to lipids, polysaccharides, and peptides (protein + peptidoglycan) for the outer part of the bacterial cell wall.
27311675	5	34	theme	gram-negative	898:910	arg1	bacteria					912:919	gram-negative bacteria	898:919	gram-negative bacteria	898:919	Thus, it is expected that for gram-negative bacteria only the outer membrane and part of the periplasmic peptidoglycan layer is probed during analysis, and for gram-positive bacteria only the top nanometers of the peptidoglycan layer of the cell wall is monitored.
27311675	0	35	theme	Composition	44:54	arg1	Analysis					0:7	Analysis	0:7	Analysis of Bacterial Cell Surface Chemical Composition	0:54	Analysis of Bacterial Cell Surface Chemical Composition Using Cryogenic X-Ray Photoelectron Spectroscopy.
27311675	5	36	theme	gram-positive	1028:1040	arg1	bacteria					1042:1049	gram-positive bacteria	1028:1049	gram-positive bacteria	1028:1049	Thus, it is expected that for gram-negative bacteria only the outer membrane and part of the periplasmic peptidoglycan layer is probed during analysis, and for gram-positive bacteria only the top nanometers of the peptidoglycan layer of the cell wall is monitored.
27311675	4	37	theme	outermost	835:843	arg1	part					845:848	the outermost part	831:848	the outermost part of the cell wall	831:865	The data obtained originate from the outermost part of the cell wall.
27311675	3	38	theme	genetic	779:785	arg1	mutations					787:795	genetic mutations	779:795	genetic mutations	779:795	The method can be used to monitor changes in the cell wall composition following environmental stimuli or genetic mutations.
27311675	5	39	theme	layer	987:991	arg1	part					949:952	part	949:952	part of the periplasmic peptidoglycan layer	949:991	Thus, it is expected that for gram-negative bacteria only the outer membrane and part of the periplasmic peptidoglycan layer is probed during analysis, and for gram-positive bacteria only the top nanometers of the peptidoglycan layer of the cell wall is monitored.
27311675	5	39	theme	layer	987:991	arg1	membrane					936:943	only the outer membrane	921:943	only the outer membrane	921:943	Thus, it is expected that for gram-negative bacteria only the outer membrane and part of the periplasmic peptidoglycan layer is probed during analysis, and for gram-positive bacteria only the top nanometers of the peptidoglycan layer of the cell wall is monitored.
27311675	2	40	dep	buffer	374:379	arg1	a					372:372	a	372:372	a	372:372	Bacterial cultures grown over night are washed with a buffer or saline at controlled pH. The analysis is done on fast-frozen bacterial cell pellets obtained after centrifugation, and the analysis requires access to X-ray photoelectron spectroscopy instrumentation that can perform analyses at cryogenic temperatures (for example using liquid nitrogen).
27311675	2	41	from	pH.	405:407	arg1	saline					384:389	saline	384:389	saline	384:389	Bacterial cultures grown over night are washed with a buffer or saline at controlled pH. The analysis is done on fast-frozen bacterial cell pellets obtained after centrifugation, and the analysis requires access to X-ray photoelectron spectroscopy instrumentation that can perform analyses at cryogenic temperatures (for example using liquid nitrogen).
27311675	2	41	from	pH.	405:407	arg1	buffer					374:379	buffer	374:379	buffer	374:379	Bacterial cultures grown over night are washed with a buffer or saline at controlled pH. The analysis is done on fast-frozen bacterial cell pellets obtained after centrifugation, and the analysis requires access to X-ray photoelectron spectroscopy instrumentation that can perform analyses at cryogenic temperatures (for example using liquid nitrogen).
27311675	3	42	used	used	691:694	arg2	method					677:682	The method	673:682	The method	673:682	The method can be used to monitor changes in the cell wall composition following environmental stimuli or genetic mutations.
27311675	0	43	theme	X-Ray	72:76	arg1	Spectroscopy					92:103	Cryogenic X-Ray Photoelectron Spectroscopy	62:103	Cryogenic X-Ray Photoelectron Spectroscopy	62:103	Analysis of Bacterial Cell Surface Chemical Composition Using Cryogenic X-Ray Photoelectron Spectroscopy.
27311675	0	44	theme	Cryogenic	62:70	arg1	Spectroscopy					92:103	Cryogenic X-Ray Photoelectron Spectroscopy	62:103	Cryogenic X-Ray Photoelectron Spectroscopy	62:103	Analysis of Bacterial Cell Surface Chemical Composition Using Cryogenic X-Ray Photoelectron Spectroscopy.
27311675	5	45	theme	cell	1109:1112	arg1	wall					1114:1117	the cell wall	1105:1117	the cell wall	1105:1117	Thus, it is expected that for gram-negative bacteria only the outer membrane and part of the periplasmic peptidoglycan layer is probed during analysis, and for gram-positive bacteria only the top nanometers of the peptidoglycan layer of the cell wall is monitored.
27311675	1	46	theme	average	156:162	arg1	surface					164:170	the average surface	152:170	the average surface chemical composition	152:191	This chapter describes a method for measuring the average surface chemical composition with respect to lipids, polysaccharides, and peptides (protein + peptidoglycan) for the outer part of the bacterial cell wall.
28287033	6	0	theme	sufficient	906:915	arg1	capacity					931:938	sufficient water holding capacity	906:938	sufficient water holding capacity	906:938	The hydrogel scaffold HACF possessed a well-defined porous architecture, sufficient water holding capacity, better hemocompatibility and biodegradability.
28287033	6	0	theme	sufficient	906:915	arg1	architecture					892:903	a well-defined porous architecture	870:903	a well-defined porous architecture	870:903	The hydrogel scaffold HACF possessed a well-defined porous architecture, sufficient water holding capacity, better hemocompatibility and biodegradability.
28287033	9	1	theme	tissue	1536:1541	arg1	applications					1555:1566	various tissue engineering applications	1528:1566	various tissue engineering applications	1528:1566	The physiochemical characterization and biological responses of HACF hydrogel signifies its suitability for various tissue engineering applications.
28287033	8	2	theme	both	1269:1272	arg1	properties					1255:1264	immunomodulatory and anti-inflammatory properties	1216:1264	immunomodulatory and anti-inflammatory properties of both of these scaffolds	1216:1291	In addition, immunomodulatory and anti-inflammatory properties of both of these scaffolds were revealed by the mRNA expressions of major inflammatory marker genes in cytotoxic condition such as TNF-α, IL-6 and NF-κB.
28287033	4	3	theme	scanning	660:667	arg1	microscopy					678:687	scanning electron microscopy	660:687	scanning electron microscopy	660:687	The prepared scaffolds were characterized by Fourier transform infrared spectroscopy and scanning electron microscopy.
28287033	7	4	dep	in	1031:1032	arg1	vitro					1034:1038	vitro	1034:1038	vitro	1034:1038	The biocompatibility was confirmed through in vitro cytotoxicity studies such as MTT assay, Neutral red uptake, DAPI staining, Trypan blue dye exclusion test and direct contact assay in L929 mouse fibroblast cells.
28287033	8	5	from	expressions	1319:1329	arg1	condition					1379:1387	cytotoxic condition	1369:1387	cytotoxic condition	1369:1387	In addition, immunomodulatory and anti-inflammatory properties of both of these scaffolds were revealed by the mRNA expressions of major inflammatory marker genes in cytotoxic condition such as TNF-α, IL-6 and NF-κB.
28287033	8	6	theme	inflammatory	1340:1351	arg1	IL-6					1404:1407	IL-6	1404:1407	IL-6	1404:1407	In addition, immunomodulatory and anti-inflammatory properties of both of these scaffolds were revealed by the mRNA expressions of major inflammatory marker genes in cytotoxic condition such as TNF-α, IL-6 and NF-κB.
28287033	8	6	theme	inflammatory	1340:1351	arg1	NF-κB					1413:1417	NF-κB	1413:1417	NF-κB	1413:1417	In addition, immunomodulatory and anti-inflammatory properties of both of these scaffolds were revealed by the mRNA expressions of major inflammatory marker genes in cytotoxic condition such as TNF-α, IL-6 and NF-κB.
28287033	8	6	theme	inflammatory	1340:1351	arg1	genes					1360:1364	major inflammatory marker genes	1334:1364	major inflammatory marker genes in cytotoxic condition such as TNF-α, IL-6 and NF-κB	1334:1417	In addition, immunomodulatory and anti-inflammatory properties of both of these scaffolds were revealed by the mRNA expressions of major inflammatory marker genes in cytotoxic condition such as TNF-α, IL-6 and NF-κB.
28287033	8	6	theme	inflammatory	1340:1351	arg1	TNF-α					1397:1401	TNF-α	1397:1401	TNF-α	1397:1401	In addition, immunomodulatory and anti-inflammatory properties of both of these scaffolds were revealed by the mRNA expressions of major inflammatory marker genes in cytotoxic condition such as TNF-α, IL-6 and NF-κB.
28287033	6	7	theme	well-defined	872:883	arg1	capacity					931:938	sufficient water holding capacity	906:938	sufficient water holding capacity	906:938	The hydrogel scaffold HACF possessed a well-defined porous architecture, sufficient water holding capacity, better hemocompatibility and biodegradability.
28287033	6	7	theme	well-defined	872:883	arg1	architecture					892:903	a well-defined porous architecture	870:903	a well-defined porous architecture	870:903	The hydrogel scaffold HACF possessed a well-defined porous architecture, sufficient water holding capacity, better hemocompatibility and biodegradability.
28287033	6	7	theme	well-defined	872:883	arg1	biodegradability					970:985	biodegradability	970:985	biodegradability	970:985	The hydrogel scaffold HACF possessed a well-defined porous architecture, sufficient water holding capacity, better hemocompatibility and biodegradability.
28287033	6	7	theme	well-defined	872:883	arg1	hemocompatibility					948:964	better hemocompatibility	941:964	better hemocompatibility	941:964	The hydrogel scaffold HACF possessed a well-defined porous architecture, sufficient water holding capacity, better hemocompatibility and biodegradability.
28287033	2	8	theme	body	350:353	arg1	reactions					355:363	foreign body reactions	342:363	foreign body reactions	342:363	Biomaterials demand good biocompatibility without the possibility of inflammation or foreign body reactions.
28287033	1	9	theme	launching	222:230	arg1	applications					243:254	launching biomedical applications	222:254	launching biomedical applications	222:254	Marine biopolymer composite materials provide a technological platform for launching biomedical applications.
28287033	8	10	theme	genes	1360:1364	arg1	expressions					1319:1329	the mRNA expressions	1310:1329	the mRNA expressions of major inflammatory marker genes in cytotoxic condition such as TNF-α, IL-6 and NF-κB	1310:1417	In addition, immunomodulatory and anti-inflammatory properties of both of these scaffolds were revealed by the mRNA expressions of major inflammatory marker genes in cytotoxic condition such as TNF-α, IL-6 and NF-κB.
28287033	7	11	theme	mouse	1179:1183	arg1	cells					1196:1200	L929 mouse fibroblast cells	1174:1200	L929 mouse fibroblast cells	1174:1200	The biocompatibility was confirmed through in vitro cytotoxicity studies such as MTT assay, Neutral red uptake, DAPI staining, Trypan blue dye exclusion test and direct contact assay in L929 mouse fibroblast cells.
28287033	8	12	theme	cytotoxic	1369:1377	arg1	condition					1379:1387	cytotoxic condition	1369:1387	cytotoxic condition	1369:1387	In addition, immunomodulatory and anti-inflammatory properties of both of these scaffolds were revealed by the mRNA expressions of major inflammatory marker genes in cytotoxic condition such as TNF-α, IL-6 and NF-κB.
28287033	5	13	theme	adsorption	781:790	arg1	studies					809:815	Porosity measurement, swelling, biodegradation, hemolysis, RBC aggregation, plasma protein adsorption and cytotoxicity studies	690:815	Porosity measurement, swelling, biodegradation, hemolysis, RBC aggregation, plasma protein adsorption and cytotoxicity studies	690:815	Porosity measurement, swelling, biodegradation, hemolysis, RBC aggregation, plasma protein adsorption and cytotoxicity studies were also done.
28287033	8	14	theme	mRNA	1314:1317	arg1	expressions					1319:1329	the mRNA expressions	1310:1329	the mRNA expressions of major inflammatory marker genes in cytotoxic condition such as TNF-α, IL-6 and NF-κB	1310:1417	In addition, immunomodulatory and anti-inflammatory properties of both of these scaffolds were revealed by the mRNA expressions of major inflammatory marker genes in cytotoxic condition such as TNF-α, IL-6 and NF-κB.
28287033	7	15	theme	contact	1157:1163	arg1	assay					1165:1169	direct contact assay	1150:1169	direct contact assay	1150:1169	The biocompatibility was confirmed through in vitro cytotoxicity studies such as MTT assay, Neutral red uptake, DAPI staining, Trypan blue dye exclusion test and direct contact assay in L929 mouse fibroblast cells.
28287033	8	16	from	genes	1360:1364	arg1	condition					1379:1387	cytotoxic condition	1369:1387	cytotoxic condition	1369:1387	In addition, immunomodulatory and anti-inflammatory properties of both of these scaffolds were revealed by the mRNA expressions of major inflammatory marker genes in cytotoxic condition such as TNF-α, IL-6 and NF-κB.
28287033	5	17	theme	aggregation	753:763	arg1	studies					809:815	Porosity measurement, swelling, biodegradation, hemolysis, RBC aggregation, plasma protein adsorption and cytotoxicity studies	690:815	Porosity measurement, swelling, biodegradation, hemolysis, RBC aggregation, plasma protein adsorption and cytotoxicity studies	690:815	Porosity measurement, swelling, biodegradation, hemolysis, RBC aggregation, plasma protein adsorption and cytotoxicity studies were also done.
28287033	1	18	theme	biopolymer	154:163	arg1	materials					175:183	Marine biopolymer composite materials	147:183	Marine biopolymer composite materials	147:183	Marine biopolymer composite materials provide a technological platform for launching biomedical applications.
28287033	7	19	theme	exclusion	1131:1139	arg1	test					1141:1144	Trypan blue dye exclusion test	1115:1144	Trypan blue dye exclusion test	1115:1144	The biocompatibility was confirmed through in vitro cytotoxicity studies such as MTT assay, Neutral red uptake, DAPI staining, Trypan blue dye exclusion test and direct contact assay in L929 mouse fibroblast cells.
28287033	3	20	theme	biocomposite	397:408	arg1	hydrogels					410:418	two biocomposite hydrogels	393:418	two biocomposite hydrogels namely; HAC (hydroxyapatite, alginate & chitosan) and HACF (hydroxyapatite, alginate, chitosan & fucoidan)	393:525	In this study, we prepared two biocomposite hydrogels namely; HAC (hydroxyapatite, alginate & chitosan) and HACF (hydroxyapatite, alginate, chitosan & fucoidan) followed by calcium chloride cross linking.
28287033	3	20	theme	biocomposite	397:408	arg1	HAC					428:430	HAC	428:430	HAC (hydroxyapatite, alginate & chitosan)	428:468	In this study, we prepared two biocomposite hydrogels namely; HAC (hydroxyapatite, alginate & chitosan) and HACF (hydroxyapatite, alginate, chitosan & fucoidan) followed by calcium chloride cross linking.
28287033	3	20	theme	biocomposite	397:408	arg1	HACF					474:477	HACF	474:477	HACF (hydroxyapatite, alginate, chitosan & fucoidan)	474:525	In this study, we prepared two biocomposite hydrogels namely; HAC (hydroxyapatite, alginate & chitosan) and HACF (hydroxyapatite, alginate, chitosan & fucoidan) followed by calcium chloride cross linking.
28287033	7	21	from	assay	1165:1169	arg1	cells					1196:1200	L929 mouse fibroblast cells	1174:1200	L929 mouse fibroblast cells	1174:1200	The biocompatibility was confirmed through in vitro cytotoxicity studies such as MTT assay, Neutral red uptake, DAPI staining, Trypan blue dye exclusion test and direct contact assay in L929 mouse fibroblast cells.
28287033	7	22	theme	red	1088:1090	arg1	uptake					1092:1097	Neutral red uptake	1080:1097	Neutral red uptake	1080:1097	The biocompatibility was confirmed through in vitro cytotoxicity studies such as MTT assay, Neutral red uptake, DAPI staining, Trypan blue dye exclusion test and direct contact assay in L929 mouse fibroblast cells.
28287033	5	23	theme	plasma	766:771	arg1	adsorption					781:790	plasma protein adsorption	766:790	plasma protein adsorption	766:790	Porosity measurement, swelling, biodegradation, hemolysis, RBC aggregation, plasma protein adsorption and cytotoxicity studies were also done.
28287033	9	24	dep	characterization	1439:1454	arg1	The					1420:1422	The	1420:1422	The	1420:1422	The physiochemical characterization and biological responses of HACF hydrogel signifies its suitability for various tissue engineering applications.
28287033	0	25	theme	tissue	114:119	arg1	applications					133:144	tissue engineering applications	114:144	tissue engineering applications	114:144	Marine macromolecules cross-linked hydrogel scaffolds as physiochemically and biologically favorable entities for tissue engineering applications.
28287033	4	26	dep	transform	624:632	arg1	infrared					634:641	infrared	634:641	transform infrared spectroscopy and scanning electron microscopy	624:687	The prepared scaffolds were characterized by Fourier transform infrared spectroscopy and scanning electron microscopy.
28287033	7	27	theme	MTT	1069:1071	arg1	assay					1073:1077	MTT assay	1069:1077	MTT assay	1069:1077	The biocompatibility was confirmed through in vitro cytotoxicity studies such as MTT assay, Neutral red uptake, DAPI staining, Trypan blue dye exclusion test and direct contact assay in L929 mouse fibroblast cells.
28287033	3	28	theme	alginate	449:456	arg1	hydroxyapatite					433:446	hydroxyapatite	433:446	hydroxyapatite	433:446	In this study, we prepared two biocomposite hydrogels namely; HAC (hydroxyapatite, alginate & chitosan) and HACF (hydroxyapatite, alginate, chitosan & fucoidan) followed by calcium chloride cross linking.
28287033	3	28	theme	alginate	449:456	arg1	chitosan					460:467	alginate & chitosan	449:467	alginate & chitosan	449:467	In this study, we prepared two biocomposite hydrogels namely; HAC (hydroxyapatite, alginate & chitosan) and HACF (hydroxyapatite, alginate, chitosan & fucoidan) followed by calcium chloride cross linking.
28287033	0	29	theme	Marine	0:5	arg1	macromolecules					7:20	Marine macromolecules	0:20	Marine macromolecules	0:20	Marine macromolecules cross-linked hydrogel scaffolds as physiochemically and biologically favorable entities for tissue engineering applications.
28287033	9	30	theme	physiochemical	1424:1437	arg1	characterization					1439:1454	physiochemical characterization	1424:1454	physiochemical characterization	1424:1454	The physiochemical characterization and biological responses of HACF hydrogel signifies its suitability for various tissue engineering applications.
28287033	7	31	theme	cytotoxicity	1040:1051	arg1	test					1141:1144	Trypan blue dye exclusion test	1115:1144	Trypan blue dye exclusion test	1115:1144	The biocompatibility was confirmed through in vitro cytotoxicity studies such as MTT assay, Neutral red uptake, DAPI staining, Trypan blue dye exclusion test and direct contact assay in L929 mouse fibroblast cells.
28287033	7	31	theme	cytotoxicity	1040:1051	arg1	assay					1073:1077	MTT assay	1069:1077	MTT assay	1069:1077	The biocompatibility was confirmed through in vitro cytotoxicity studies such as MTT assay, Neutral red uptake, DAPI staining, Trypan blue dye exclusion test and direct contact assay in L929 mouse fibroblast cells.
28287033	7	31	theme	cytotoxicity	1040:1051	arg1	staining					1105:1112	DAPI staining	1100:1112	DAPI staining	1100:1112	The biocompatibility was confirmed through in vitro cytotoxicity studies such as MTT assay, Neutral red uptake, DAPI staining, Trypan blue dye exclusion test and direct contact assay in L929 mouse fibroblast cells.
28287033	7	31	theme	cytotoxicity	1040:1051	arg1	studies					1053:1059	in vitro cytotoxicity studies	1031:1059	in vitro cytotoxicity studies such as MTT assay, Neutral red uptake, DAPI staining, Trypan blue dye exclusion test and direct contact assay in L929 mouse fibroblast cells	1031:1200	The biocompatibility was confirmed through in vitro cytotoxicity studies such as MTT assay, Neutral red uptake, DAPI staining, Trypan blue dye exclusion test and direct contact assay in L929 mouse fibroblast cells.
28287033	7	31	theme	cytotoxicity	1040:1051	arg1	assay					1165:1169	direct contact assay	1150:1169	direct contact assay	1150:1169	The biocompatibility was confirmed through in vitro cytotoxicity studies such as MTT assay, Neutral red uptake, DAPI staining, Trypan blue dye exclusion test and direct contact assay in L929 mouse fibroblast cells.
28287033	7	31	theme	cytotoxicity	1040:1051	arg1	uptake					1092:1097	Neutral red uptake	1080:1097	Neutral red uptake	1080:1097	The biocompatibility was confirmed through in vitro cytotoxicity studies such as MTT assay, Neutral red uptake, DAPI staining, Trypan blue dye exclusion test and direct contact assay in L929 mouse fibroblast cells.
28287033	7	32	theme	fibroblast	1185:1194	arg1	cells					1196:1200	L929 mouse fibroblast cells	1174:1200	L929 mouse fibroblast cells	1174:1200	The biocompatibility was confirmed through in vitro cytotoxicity studies such as MTT assay, Neutral red uptake, DAPI staining, Trypan blue dye exclusion test and direct contact assay in L929 mouse fibroblast cells.
28287033	3	33	dep	prepared	384:391	arg1	followed					527:534	followed	527:534	followed by calcium chloride cross linking	527:568	In this study, we prepared two biocomposite hydrogels namely; HAC (hydroxyapatite, alginate & chitosan) and HACF (hydroxyapatite, alginate, chitosan & fucoidan) followed by calcium chloride cross linking.
28287033	3	34	theme	&	515:515	arg1	hydroxyapatite					480:493	hydroxyapatite	480:493	hydroxyapatite	480:493	In this study, we prepared two biocomposite hydrogels namely; HAC (hydroxyapatite, alginate & chitosan) and HACF (hydroxyapatite, alginate, chitosan & fucoidan) followed by calcium chloride cross linking.
28287033	3	34	theme	&	515:515	arg1	fucoidan					517:524	chitosan & fucoidan	506:524	chitosan & fucoidan	506:524	In this study, we prepared two biocomposite hydrogels namely; HAC (hydroxyapatite, alginate & chitosan) and HACF (hydroxyapatite, alginate, chitosan & fucoidan) followed by calcium chloride cross linking.
28287033	8	35	from	condition	1379:1387	arg1	expressions					1319:1329	the mRNA expressions	1310:1329	the mRNA expressions of major inflammatory marker genes in cytotoxic condition such as TNF-α, IL-6 and NF-κB	1310:1417	In addition, immunomodulatory and anti-inflammatory properties of both of these scaffolds were revealed by the mRNA expressions of major inflammatory marker genes in cytotoxic condition such as TNF-α, IL-6 and NF-κB.
28287033	6	36	theme	hydrogel	837:844	arg1	HACF					855:858	The hydrogel scaffold HACF	833:858	The hydrogel scaffold HACF	833:858	The hydrogel scaffold HACF possessed a well-defined porous architecture, sufficient water holding capacity, better hemocompatibility and biodegradability.
28287033	9	37	theme	HACF	1484:1487	arg1	hydrogel					1489:1496	HACF hydrogel	1484:1496	HACF hydrogel	1484:1496	The physiochemical characterization and biological responses of HACF hydrogel signifies its suitability for various tissue engineering applications.
28287033	3	38	dep	HAC	428:430	arg1	hydroxyapatite					433:446	hydroxyapatite	433:446	hydroxyapatite	433:446	In this study, we prepared two biocomposite hydrogels namely; HAC (hydroxyapatite, alginate & chitosan) and HACF (hydroxyapatite, alginate, chitosan & fucoidan) followed by calcium chloride cross linking.
28287033	3	38	dep	HAC	428:430	arg1	chitosan					460:467	alginate & chitosan	449:467	alginate & chitosan	449:467	In this study, we prepared two biocomposite hydrogels namely; HAC (hydroxyapatite, alginate & chitosan) and HACF (hydroxyapatite, alginate, chitosan & fucoidan) followed by calcium chloride cross linking.
28287033	6	39	theme	holding	923:929	arg1	capacity					931:938	sufficient water holding capacity	906:938	sufficient water holding capacity	906:938	The hydrogel scaffold HACF possessed a well-defined porous architecture, sufficient water holding capacity, better hemocompatibility and biodegradability.
28287033	6	39	theme	holding	923:929	arg1	architecture					892:903	a well-defined porous architecture	870:903	a well-defined porous architecture	870:903	The hydrogel scaffold HACF possessed a well-defined porous architecture, sufficient water holding capacity, better hemocompatibility and biodegradability.
28287033	4	40	theme	electron	669:676	arg1	microscopy					678:687	scanning electron microscopy	660:687	scanning electron microscopy	660:687	The prepared scaffolds were characterized by Fourier transform infrared spectroscopy and scanning electron microscopy.
28287033	3	41	theme	linking	562:568	arg1	cross					556:560	cross linking	556:568	calcium chloride cross linking	539:568	In this study, we prepared two biocomposite hydrogels namely; HAC (hydroxyapatite, alginate & chitosan) and HACF (hydroxyapatite, alginate, chitosan & fucoidan) followed by calcium chloride cross linking.
28287033	7	42	from	uptake	1092:1097	arg1	cells					1196:1200	L929 mouse fibroblast cells	1174:1200	L929 mouse fibroblast cells	1174:1200	The biocompatibility was confirmed through in vitro cytotoxicity studies such as MTT assay, Neutral red uptake, DAPI staining, Trypan blue dye exclusion test and direct contact assay in L929 mouse fibroblast cells.
28287033	8	43	theme	immunomodulatory	1216:1231	arg1	properties					1255:1264	immunomodulatory and anti-inflammatory properties	1216:1264	immunomodulatory and anti-inflammatory properties of both of these scaffolds	1216:1291	In addition, immunomodulatory and anti-inflammatory properties of both of these scaffolds were revealed by the mRNA expressions of major inflammatory marker genes in cytotoxic condition such as TNF-α, IL-6 and NF-κB.
28287033	5	44	theme	biodegradation	722:735	arg1	studies					809:815	Porosity measurement, swelling, biodegradation, hemolysis, RBC aggregation, plasma protein adsorption and cytotoxicity studies	690:815	Porosity measurement, swelling, biodegradation, hemolysis, RBC aggregation, plasma protein adsorption and cytotoxicity studies	690:815	Porosity measurement, swelling, biodegradation, hemolysis, RBC aggregation, plasma protein adsorption and cytotoxicity studies were also done.
28287033	9	45	theme	various	1528:1534	arg1	applications					1555:1566	various tissue engineering applications	1528:1566	various tissue engineering applications	1528:1566	The physiochemical characterization and biological responses of HACF hydrogel signifies its suitability for various tissue engineering applications.
28287033	8	46	theme	anti-inflammatory	1237:1253	arg1	properties					1255:1264	immunomodulatory and anti-inflammatory properties	1216:1264	immunomodulatory and anti-inflammatory properties of both of these scaffolds	1216:1291	In addition, immunomodulatory and anti-inflammatory properties of both of these scaffolds were revealed by the mRNA expressions of major inflammatory marker genes in cytotoxic condition such as TNF-α, IL-6 and NF-κB.
28287033	5	47	theme	swelling	712:719	arg1	studies					809:815	Porosity measurement, swelling, biodegradation, hemolysis, RBC aggregation, plasma protein adsorption and cytotoxicity studies	690:815	Porosity measurement, swelling, biodegradation, hemolysis, RBC aggregation, plasma protein adsorption and cytotoxicity studies	690:815	Porosity measurement, swelling, biodegradation, hemolysis, RBC aggregation, plasma protein adsorption and cytotoxicity studies were also done.
28287033	5	48	theme	hemolysis	738:746	arg1	studies					809:815	Porosity measurement, swelling, biodegradation, hemolysis, RBC aggregation, plasma protein adsorption and cytotoxicity studies	690:815	Porosity measurement, swelling, biodegradation, hemolysis, RBC aggregation, plasma protein adsorption and cytotoxicity studies	690:815	Porosity measurement, swelling, biodegradation, hemolysis, RBC aggregation, plasma protein adsorption and cytotoxicity studies were also done.
28287033	9	49	theme	engineering	1543:1553	arg1	applications					1555:1566	various tissue engineering applications	1528:1566	various tissue engineering applications	1528:1566	The physiochemical characterization and biological responses of HACF hydrogel signifies its suitability for various tissue engineering applications.
28287033	5	50	theme	measurement	699:709	arg1	studies					809:815	Porosity measurement, swelling, biodegradation, hemolysis, RBC aggregation, plasma protein adsorption and cytotoxicity studies	690:815	Porosity measurement, swelling, biodegradation, hemolysis, RBC aggregation, plasma protein adsorption and cytotoxicity studies	690:815	Porosity measurement, swelling, biodegradation, hemolysis, RBC aggregation, plasma protein adsorption and cytotoxicity studies were also done.
28287033	6	51	theme	porous	885:890	arg1	capacity					931:938	sufficient water holding capacity	906:938	sufficient water holding capacity	906:938	The hydrogel scaffold HACF possessed a well-defined porous architecture, sufficient water holding capacity, better hemocompatibility and biodegradability.
28287033	6	51	theme	porous	885:890	arg1	architecture					892:903	a well-defined porous architecture	870:903	a well-defined porous architecture	870:903	The hydrogel scaffold HACF possessed a well-defined porous architecture, sufficient water holding capacity, better hemocompatibility and biodegradability.
28287033	6	51	theme	porous	885:890	arg1	biodegradability					970:985	biodegradability	970:985	biodegradability	970:985	The hydrogel scaffold HACF possessed a well-defined porous architecture, sufficient water holding capacity, better hemocompatibility and biodegradability.
28287033	6	51	theme	porous	885:890	arg1	hemocompatibility					948:964	better hemocompatibility	941:964	better hemocompatibility	941:964	The hydrogel scaffold HACF possessed a well-defined porous architecture, sufficient water holding capacity, better hemocompatibility and biodegradability.
28287033	2	52	theme	reactions	355:363	arg1	possibility					311:321	the possibility	307:321	the possibility of inflammation or foreign body reactions	307:363	Biomaterials demand good biocompatibility without the possibility of inflammation or foreign body reactions.
28287033	8	53	theme	major	1334:1338	arg1	IL-6					1404:1407	IL-6	1404:1407	IL-6	1404:1407	In addition, immunomodulatory and anti-inflammatory properties of both of these scaffolds were revealed by the mRNA expressions of major inflammatory marker genes in cytotoxic condition such as TNF-α, IL-6 and NF-κB.
28287033	8	53	theme	major	1334:1338	arg1	NF-κB					1413:1417	NF-κB	1413:1417	NF-κB	1413:1417	In addition, immunomodulatory and anti-inflammatory properties of both of these scaffolds were revealed by the mRNA expressions of major inflammatory marker genes in cytotoxic condition such as TNF-α, IL-6 and NF-κB.
28287033	8	53	theme	major	1334:1338	arg1	genes					1360:1364	major inflammatory marker genes	1334:1364	major inflammatory marker genes in cytotoxic condition such as TNF-α, IL-6 and NF-κB	1334:1417	In addition, immunomodulatory and anti-inflammatory properties of both of these scaffolds were revealed by the mRNA expressions of major inflammatory marker genes in cytotoxic condition such as TNF-α, IL-6 and NF-κB.
28287033	8	53	theme	major	1334:1338	arg1	TNF-α					1397:1401	TNF-α	1397:1401	TNF-α	1397:1401	In addition, immunomodulatory and anti-inflammatory properties of both of these scaffolds were revealed by the mRNA expressions of major inflammatory marker genes in cytotoxic condition such as TNF-α, IL-6 and NF-κB.
28287033	7	54	theme	L929	1174:1177	arg1	cells					1196:1200	L929 mouse fibroblast cells	1174:1200	L929 mouse fibroblast cells	1174:1200	The biocompatibility was confirmed through in vitro cytotoxicity studies such as MTT assay, Neutral red uptake, DAPI staining, Trypan blue dye exclusion test and direct contact assay in L929 mouse fibroblast cells.
28287033	1	55	theme	biomedical	232:241	arg1	applications					243:254	launching biomedical applications	222:254	launching biomedical applications	222:254	Marine biopolymer composite materials provide a technological platform for launching biomedical applications.
28287033	8	56	theme	marker	1353:1358	arg1	IL-6					1404:1407	IL-6	1404:1407	IL-6	1404:1407	In addition, immunomodulatory and anti-inflammatory properties of both of these scaffolds were revealed by the mRNA expressions of major inflammatory marker genes in cytotoxic condition such as TNF-α, IL-6 and NF-κB.
28287033	8	56	theme	marker	1353:1358	arg1	NF-κB					1413:1417	NF-κB	1413:1417	NF-κB	1413:1417	In addition, immunomodulatory and anti-inflammatory properties of both of these scaffolds were revealed by the mRNA expressions of major inflammatory marker genes in cytotoxic condition such as TNF-α, IL-6 and NF-κB.
28287033	8	56	theme	marker	1353:1358	arg1	genes					1360:1364	major inflammatory marker genes	1334:1364	major inflammatory marker genes in cytotoxic condition such as TNF-α, IL-6 and NF-κB	1334:1417	In addition, immunomodulatory and anti-inflammatory properties of both of these scaffolds were revealed by the mRNA expressions of major inflammatory marker genes in cytotoxic condition such as TNF-α, IL-6 and NF-κB.
28287033	8	56	theme	marker	1353:1358	arg1	TNF-α					1397:1401	TNF-α	1397:1401	TNF-α	1397:1401	In addition, immunomodulatory and anti-inflammatory properties of both of these scaffolds were revealed by the mRNA expressions of major inflammatory marker genes in cytotoxic condition such as TNF-α, IL-6 and NF-κB.
28287033	5	57	theme	protein	773:779	arg1	adsorption					781:790	plasma protein adsorption	766:790	plasma protein adsorption	766:790	Porosity measurement, swelling, biodegradation, hemolysis, RBC aggregation, plasma protein adsorption and cytotoxicity studies were also done.
28287033	7	58	theme	direct	1150:1155	arg1	contact					1157:1163	direct contact	1150:1163	direct contact assay	1150:1169	The biocompatibility was confirmed through in vitro cytotoxicity studies such as MTT assay, Neutral red uptake, DAPI staining, Trypan blue dye exclusion test and direct contact assay in L929 mouse fibroblast cells.
28287033	5	59	theme	RBC	749:751	arg1	aggregation					753:763	RBC aggregation	749:763	RBC aggregation	749:763	Porosity measurement, swelling, biodegradation, hemolysis, RBC aggregation, plasma protein adsorption and cytotoxicity studies were also done.
28287033	7	60	theme	blue	1122:1125	arg1	exclusion					1131:1139	Trypan blue dye exclusion	1115:1139	Trypan blue dye exclusion test	1115:1144	The biocompatibility was confirmed through in vitro cytotoxicity studies such as MTT assay, Neutral red uptake, DAPI staining, Trypan blue dye exclusion test and direct contact assay in L929 mouse fibroblast cells.
28287033	1	61	theme	Marine	147:152	arg1	materials					175:183	Marine biopolymer composite materials	147:183	Marine biopolymer composite materials	147:183	Marine biopolymer composite materials provide a technological platform for launching biomedical applications.
28287033	7	62	theme	dye	1127:1129	arg1	exclusion					1131:1139	Trypan blue dye exclusion	1115:1139	Trypan blue dye exclusion test	1115:1144	The biocompatibility was confirmed through in vitro cytotoxicity studies such as MTT assay, Neutral red uptake, DAPI staining, Trypan blue dye exclusion test and direct contact assay in L929 mouse fibroblast cells.
28287033	2	63	theme	foreign	342:348	arg1	reactions					355:363	foreign body reactions	342:363	foreign body reactions	342:363	Biomaterials demand good biocompatibility without the possibility of inflammation or foreign body reactions.
28287033	1	64	theme	composite	165:173	arg1	materials					175:183	Marine biopolymer composite materials	147:183	Marine biopolymer composite materials	147:183	Marine biopolymer composite materials provide a technological platform for launching biomedical applications.
28287033	0	65	theme	favorable	91:99	arg1	entities					101:108	physiochemically and biologically favorable entities	57:108	physiochemically and biologically favorable entities	57:108	Marine macromolecules cross-linked hydrogel scaffolds as physiochemically and biologically favorable entities for tissue engineering applications.
28287033	2	66	theme	inflammation	326:337	arg1	possibility					311:321	the possibility	307:321	the possibility of inflammation or foreign body reactions	307:363	Biomaterials demand good biocompatibility without the possibility of inflammation or foreign body reactions.
28287033	7	67	from	staining	1105:1112	arg1	cells					1196:1200	L929 mouse fibroblast cells	1174:1200	L929 mouse fibroblast cells	1174:1200	The biocompatibility was confirmed through in vitro cytotoxicity studies such as MTT assay, Neutral red uptake, DAPI staining, Trypan blue dye exclusion test and direct contact assay in L929 mouse fibroblast cells.
28287033	7	68	from	assay	1073:1077	arg1	cells					1196:1200	L929 mouse fibroblast cells	1174:1200	L929 mouse fibroblast cells	1174:1200	The biocompatibility was confirmed through in vitro cytotoxicity studies such as MTT assay, Neutral red uptake, DAPI staining, Trypan blue dye exclusion test and direct contact assay in L929 mouse fibroblast cells.
28287033	1	69	theme	technological	195:207	arg1	platform					209:216	a technological platform	193:216	a technological platform for launching biomedical applications	193:254	Marine biopolymer composite materials provide a technological platform for launching biomedical applications.
28287033	0	70	theme	engineering	121:131	arg1	applications					133:144	tissue engineering applications	114:144	tissue engineering applications	114:144	Marine macromolecules cross-linked hydrogel scaffolds as physiochemically and biologically favorable entities for tissue engineering applications.
28287033	7	71	theme	Neutral	1080:1086	arg1	uptake					1092:1097	Neutral red uptake	1080:1097	Neutral red uptake	1080:1097	The biocompatibility was confirmed through in vitro cytotoxicity studies such as MTT assay, Neutral red uptake, DAPI staining, Trypan blue dye exclusion test and direct contact assay in L929 mouse fibroblast cells.
28287033	6	72	contain	possessed	860:868	arg2	biodegradability					970:985	biodegradability	970:985	biodegradability	970:985	The hydrogel scaffold HACF possessed a well-defined porous architecture, sufficient water holding capacity, better hemocompatibility and biodegradability.
28287033	6	72	contain	possessed	860:868	arg1	HACF					855:858	The hydrogel scaffold HACF	833:858	The hydrogel scaffold HACF	833:858	The hydrogel scaffold HACF possessed a well-defined porous architecture, sufficient water holding capacity, better hemocompatibility and biodegradability.
28287033	6	72	contain	possessed	860:868	arg2	hemocompatibility					948:964	better hemocompatibility	941:964	better hemocompatibility	941:964	The hydrogel scaffold HACF possessed a well-defined porous architecture, sufficient water holding capacity, better hemocompatibility and biodegradability.
28287033	6	72	contain	possessed	860:868	arg2	architecture					892:903	a well-defined porous architecture	870:903	a well-defined porous architecture	870:903	The hydrogel scaffold HACF possessed a well-defined porous architecture, sufficient water holding capacity, better hemocompatibility and biodegradability.
28287033	6	72	contain	possessed	860:868	arg2	capacity					931:938	sufficient water holding capacity	906:938	sufficient water holding capacity	906:938	The hydrogel scaffold HACF possessed a well-defined porous architecture, sufficient water holding capacity, better hemocompatibility and biodegradability.
28287033	4	73	dep	Fourier	616:622	arg1	transform					624:632	transform	624:632	transform infrared spectroscopy and scanning electron microscopy	624:687	The prepared scaffolds were characterized by Fourier transform infrared spectroscopy and scanning electron microscopy.
28287033	5	74	theme	Porosity	690:697	arg1	studies					809:815	Porosity measurement, swelling, biodegradation, hemolysis, RBC aggregation, plasma protein adsorption and cytotoxicity studies	690:815	Porosity measurement, swelling, biodegradation, hemolysis, RBC aggregation, plasma protein adsorption and cytotoxicity studies	690:815	Porosity measurement, swelling, biodegradation, hemolysis, RBC aggregation, plasma protein adsorption and cytotoxicity studies were also done.
28287033	7	75	theme	DAPI	1100:1103	arg1	staining					1105:1112	DAPI staining	1100:1112	DAPI staining	1100:1112	The biocompatibility was confirmed through in vitro cytotoxicity studies such as MTT assay, Neutral red uptake, DAPI staining, Trypan blue dye exclusion test and direct contact assay in L929 mouse fibroblast cells.
28287033	3	76	dep	chloride	547:554	arg1	cross					556:560	cross linking	556:568	calcium chloride cross linking	539:568	In this study, we prepared two biocomposite hydrogels namely; HAC (hydroxyapatite, alginate & chitosan) and HACF (hydroxyapatite, alginate, chitosan & fucoidan) followed by calcium chloride cross linking.
28287033	3	77	theme	&	458:458	arg1	hydroxyapatite					433:446	hydroxyapatite	433:446	hydroxyapatite	433:446	In this study, we prepared two biocomposite hydrogels namely; HAC (hydroxyapatite, alginate & chitosan) and HACF (hydroxyapatite, alginate, chitosan & fucoidan) followed by calcium chloride cross linking.
28287033	3	77	theme	&	458:458	arg1	chitosan					460:467	alginate & chitosan	449:467	alginate & chitosan	449:467	In this study, we prepared two biocomposite hydrogels namely; HAC (hydroxyapatite, alginate & chitosan) and HACF (hydroxyapatite, alginate, chitosan & fucoidan) followed by calcium chloride cross linking.
28287033	3	78	dep	HACF	474:477	arg1	alginate					496:503	alginate	496:503	alginate	496:503	In this study, we prepared two biocomposite hydrogels namely; HAC (hydroxyapatite, alginate & chitosan) and HACF (hydroxyapatite, alginate, chitosan & fucoidan) followed by calcium chloride cross linking.
28287033	3	78	dep	HACF	474:477	arg1	hydroxyapatite					480:493	hydroxyapatite	480:493	hydroxyapatite	480:493	In this study, we prepared two biocomposite hydrogels namely; HAC (hydroxyapatite, alginate & chitosan) and HACF (hydroxyapatite, alginate, chitosan & fucoidan) followed by calcium chloride cross linking.
28287033	3	78	dep	HACF	474:477	arg1	fucoidan					517:524	chitosan & fucoidan	506:524	chitosan & fucoidan	506:524	In this study, we prepared two biocomposite hydrogels namely; HAC (hydroxyapatite, alginate & chitosan) and HACF (hydroxyapatite, alginate, chitosan & fucoidan) followed by calcium chloride cross linking.
28287033	0	79	theme	hydrogel	35:42	arg1	scaffolds					44:52	hydrogel scaffolds	35:52	hydrogel scaffolds as physiochemically and biologically favorable entities for tissue engineering applications	35:144	Marine macromolecules cross-linked hydrogel scaffolds as physiochemically and biologically favorable entities for tissue engineering applications.
28287033	9	80	theme	biological	1460:1469	arg1	responses					1471:1479	biological responses	1460:1479	biological responses	1460:1479	The physiochemical characterization and biological responses of HACF hydrogel signifies its suitability for various tissue engineering applications.
28287033	4	81	theme	prepared	575:582	arg1	scaffolds					584:592	The prepared scaffolds	571:592	The prepared scaffolds	571:592	The prepared scaffolds were characterized by Fourier transform infrared spectroscopy and scanning electron microscopy.
28287033	3	82	dep	hydrogels	410:418	arg1	hydrogels					410:418	two biocomposite hydrogels	393:418	two biocomposite hydrogels namely; HAC (hydroxyapatite, alginate & chitosan) and HACF (hydroxyapatite, alginate, chitosan & fucoidan)	393:525	In this study, we prepared two biocomposite hydrogels namely; HAC (hydroxyapatite, alginate & chitosan) and HACF (hydroxyapatite, alginate, chitosan & fucoidan) followed by calcium chloride cross linking.
28287033	3	82	dep	hydrogels	410:418	arg1	HAC					428:430	HAC	428:430	HAC (hydroxyapatite, alginate & chitosan)	428:468	In this study, we prepared two biocomposite hydrogels namely; HAC (hydroxyapatite, alginate & chitosan) and HACF (hydroxyapatite, alginate, chitosan & fucoidan) followed by calcium chloride cross linking.
28287033	3	82	dep	hydrogels	410:418	arg1	HACF					474:477	HACF	474:477	HACF (hydroxyapatite, alginate, chitosan & fucoidan)	474:525	In this study, we prepared two biocomposite hydrogels namely; HAC (hydroxyapatite, alginate & chitosan) and HACF (hydroxyapatite, alginate, chitosan & fucoidan) followed by calcium chloride cross linking.
28287033	6	83	theme	scaffold	846:853	arg1	HACF					855:858	The hydrogel scaffold HACF	833:858	The hydrogel scaffold HACF	833:858	The hydrogel scaffold HACF possessed a well-defined porous architecture, sufficient water holding capacity, better hemocompatibility and biodegradability.
28287033	3	84	theme	chitosan	506:513	arg1	hydroxyapatite					480:493	hydroxyapatite	480:493	hydroxyapatite	480:493	In this study, we prepared two biocomposite hydrogels namely; HAC (hydroxyapatite, alginate & chitosan) and HACF (hydroxyapatite, alginate, chitosan & fucoidan) followed by calcium chloride cross linking.
28287033	3	84	theme	chitosan	506:513	arg1	fucoidan					517:524	chitosan & fucoidan	506:524	chitosan & fucoidan	506:524	In this study, we prepared two biocomposite hydrogels namely; HAC (hydroxyapatite, alginate & chitosan) and HACF (hydroxyapatite, alginate, chitosan & fucoidan) followed by calcium chloride cross linking.
28287033	6	85	theme	better	941:946	arg1	hemocompatibility					948:964	better hemocompatibility	941:964	better hemocompatibility	941:964	The hydrogel scaffold HACF possessed a well-defined porous architecture, sufficient water holding capacity, better hemocompatibility and biodegradability.
28287033	6	85	theme	better	941:946	arg1	architecture					892:903	a well-defined porous architecture	870:903	a well-defined porous architecture	870:903	The hydrogel scaffold HACF possessed a well-defined porous architecture, sufficient water holding capacity, better hemocompatibility and biodegradability.
28287033	5	86	theme	cytotoxicity	796:807	arg1	studies					809:815	Porosity measurement, swelling, biodegradation, hemolysis, RBC aggregation, plasma protein adsorption and cytotoxicity studies	690:815	Porosity measurement, swelling, biodegradation, hemolysis, RBC aggregation, plasma protein adsorption and cytotoxicity studies	690:815	Porosity measurement, swelling, biodegradation, hemolysis, RBC aggregation, plasma protein adsorption and cytotoxicity studies were also done.
28287033	2	87	theme	good	277:280	arg1	biocompatibility					282:297	good biocompatibility	277:297	good biocompatibility	277:297	Biomaterials demand good biocompatibility without the possibility of inflammation or foreign body reactions.
28287033	3	88	theme	calcium	539:545	arg1	chloride					547:554	calcium chloride cross linking	539:568	calcium chloride cross linking	539:568	In this study, we prepared two biocomposite hydrogels namely; HAC (hydroxyapatite, alginate & chitosan) and HACF (hydroxyapatite, alginate, chitosan & fucoidan) followed by calcium chloride cross linking.
28287033	7	89	theme	in	1031:1032	arg1	test					1141:1144	Trypan blue dye exclusion test	1115:1144	Trypan blue dye exclusion test	1115:1144	The biocompatibility was confirmed through in vitro cytotoxicity studies such as MTT assay, Neutral red uptake, DAPI staining, Trypan blue dye exclusion test and direct contact assay in L929 mouse fibroblast cells.
28287033	7	89	theme	in	1031:1032	arg1	assay					1073:1077	MTT assay	1069:1077	MTT assay	1069:1077	The biocompatibility was confirmed through in vitro cytotoxicity studies such as MTT assay, Neutral red uptake, DAPI staining, Trypan blue dye exclusion test and direct contact assay in L929 mouse fibroblast cells.
28287033	7	89	theme	in	1031:1032	arg1	staining					1105:1112	DAPI staining	1100:1112	DAPI staining	1100:1112	The biocompatibility was confirmed through in vitro cytotoxicity studies such as MTT assay, Neutral red uptake, DAPI staining, Trypan blue dye exclusion test and direct contact assay in L929 mouse fibroblast cells.
28287033	7	89	theme	in	1031:1032	arg1	studies					1053:1059	in vitro cytotoxicity studies	1031:1059	in vitro cytotoxicity studies such as MTT assay, Neutral red uptake, DAPI staining, Trypan blue dye exclusion test and direct contact assay in L929 mouse fibroblast cells	1031:1200	The biocompatibility was confirmed through in vitro cytotoxicity studies such as MTT assay, Neutral red uptake, DAPI staining, Trypan blue dye exclusion test and direct contact assay in L929 mouse fibroblast cells.
28287033	7	89	theme	in	1031:1032	arg1	assay					1165:1169	direct contact assay	1150:1169	direct contact assay	1150:1169	The biocompatibility was confirmed through in vitro cytotoxicity studies such as MTT assay, Neutral red uptake, DAPI staining, Trypan blue dye exclusion test and direct contact assay in L929 mouse fibroblast cells.
28287033	7	89	theme	in	1031:1032	arg1	uptake					1092:1097	Neutral red uptake	1080:1097	Neutral red uptake	1080:1097	The biocompatibility was confirmed through in vitro cytotoxicity studies such as MTT assay, Neutral red uptake, DAPI staining, Trypan blue dye exclusion test and direct contact assay in L929 mouse fibroblast cells.
28287033	9	90	theme	hydrogel	1489:1496	arg1	characterization					1439:1454	physiochemical characterization	1424:1454	physiochemical characterization	1424:1454	The physiochemical characterization and biological responses of HACF hydrogel signifies its suitability for various tissue engineering applications.
28287033	9	90	theme	hydrogel	1489:1496	arg1	responses					1471:1479	biological responses	1460:1479	biological responses	1460:1479	The physiochemical characterization and biological responses of HACF hydrogel signifies its suitability for various tissue engineering applications.
28287033	7	91	theme	Trypan	1115:1120	arg1	exclusion					1131:1139	Trypan blue dye exclusion	1115:1139	Trypan blue dye exclusion test	1115:1144	The biocompatibility was confirmed through in vitro cytotoxicity studies such as MTT assay, Neutral red uptake, DAPI staining, Trypan blue dye exclusion test and direct contact assay in L929 mouse fibroblast cells.
28287033	6	92	theme	water	917:921	arg1	capacity					931:938	sufficient water holding capacity	906:938	sufficient water holding capacity	906:938	The hydrogel scaffold HACF possessed a well-defined porous architecture, sufficient water holding capacity, better hemocompatibility and biodegradability.
28287033	6	92	theme	water	917:921	arg1	architecture					892:903	a well-defined porous architecture	870:903	a well-defined porous architecture	870:903	The hydrogel scaffold HACF possessed a well-defined porous architecture, sufficient water holding capacity, better hemocompatibility and biodegradability.
28287033	7	93	from	test	1141:1144	arg1	cells					1196:1200	L929 mouse fibroblast cells	1174:1200	L929 mouse fibroblast cells	1174:1200	The biocompatibility was confirmed through in vitro cytotoxicity studies such as MTT assay, Neutral red uptake, DAPI staining, Trypan blue dye exclusion test and direct contact assay in L929 mouse fibroblast cells.
26572417	5	0	theme	conventional	724:735	arg1	loading					741:747	conventional PCC loading	724:747	conventional PCC loading	724:747	The paper filled with the composite fillers had much higher bursting and tensile strengths than conventional PCC loading.
26572417	6	1	theme	paper	875:879	arg1	content					864:870	the filler content	853:870	the filler content of paper	853:879	It was also found that the paper prepared with PCC-CNF composite fillers became denser with increasing the filler content of paper.
26572417	1	2	theme	cellulose	159:167	arg1	CNF					182:184	CNF	182:184	CNF	182:184	A new concept of composite filler was developed by using cellulose nanofibrils (CNF), precipitated calcium carbonate (PCC) and cationic starch (C-starch).
26572417	1	2	theme	cellulose	159:167	arg1	nanofibrils					169:179	cellulose nanofibrils	159:179	cellulose nanofibrils (CNF)	159:185	A new concept of composite filler was developed by using cellulose nanofibrils (CNF), precipitated calcium carbonate (PCC) and cationic starch (C-starch).
26572417	4	3	theme	filler	543:548	arg1	retention					550:558	The highest filler retention	531:558	The highest filler retention	531:558	The highest filler retention was obtained by using the PCC-CNF composite filler in paper sheets.
26572417	5	4	theme	PCC	737:739	arg1	loading					741:747	conventional PCC loading	724:747	conventional PCC loading	724:747	The paper filled with the composite fillers had much higher bursting and tensile strengths than conventional PCC loading.
26572417	3	5	dep	strength	484:491	arg1	the					480:482	the	480:482	the	480:482	The aim was to elucidate their effects on flocculation, filler retention and the strength and optical properties of handsheets.
26572417	4	6	theme	highest	535:541	arg1	retention					550:558	The highest filler retention	531:558	The highest filler retention	531:558	The highest filler retention was obtained by using the PCC-CNF composite filler in paper sheets.
26572417	5	7	theme	higher	681:686	arg1	bursting					688:695	much higher bursting	676:695	much higher bursting	676:695	The paper filled with the composite fillers had much higher bursting and tensile strengths than conventional PCC loading.
26572417	3	8	theme	optical	497:503	arg1	properties					505:514	optical properties	497:514	optical properties	497:514	The aim was to elucidate their effects on flocculation, filler retention and the strength and optical properties of handsheets.
26572417	6	9	theme	filler	857:862	arg1	content					864:870	the filler content	853:870	the filler content of paper	853:879	It was also found that the paper prepared with PCC-CNF composite fillers became denser with increasing the filler content of paper.
26572417	2	10	dep	ways	325:328	arg1	ways					325:328	two different ways	311:328	two different ways: a PCC-CNF composite filler and a papermaking additive in sheet forming	311:400	In this study, cellulose nanofibrils were utilized in two different ways: a PCC-CNF composite filler and a papermaking additive in sheet forming.
26572417	2	10	dep	ways	325:328	arg1	filler					351:356	a PCC-CNF composite filler	331:356	a PCC-CNF composite filler	331:356	In this study, cellulose nanofibrils were utilized in two different ways: a PCC-CNF composite filler and a papermaking additive in sheet forming.
26572417	2	10	dep	ways	325:328	arg1	papermaking					364:374	a papermaking	362:374	a papermaking additive in sheet forming	362:400	In this study, cellulose nanofibrils were utilized in two different ways: a PCC-CNF composite filler and a papermaking additive in sheet forming.
26572417	2	11	theme	different	315:323	arg1	papermaking					364:374	a papermaking	362:374	a papermaking additive in sheet forming	362:400	In this study, cellulose nanofibrils were utilized in two different ways: a PCC-CNF composite filler and a papermaking additive in sheet forming.
26572417	2	11	theme	different	315:323	arg1	filler					351:356	a PCC-CNF composite filler	331:356	a PCC-CNF composite filler	331:356	In this study, cellulose nanofibrils were utilized in two different ways: a PCC-CNF composite filler and a papermaking additive in sheet forming.
26572417	2	11	theme	different	315:323	arg1	ways					325:328	two different ways	311:328	two different ways: a PCC-CNF composite filler and a papermaking additive in sheet forming	311:400	In this study, cellulose nanofibrils were utilized in two different ways: a PCC-CNF composite filler and a papermaking additive in sheet forming.
26572417	4	12	theme	PCC-CNF	586:592	arg1	filler					604:609	the PCC-CNF composite filler	582:609	the PCC-CNF composite filler in paper sheets	582:625	The highest filler retention was obtained by using the PCC-CNF composite filler in paper sheets.
26572417	5	13	theme	tensile	701:707	arg1	strengths					709:717	tensile strengths	701:717	tensile strengths	701:717	The paper filled with the composite fillers had much higher bursting and tensile strengths than conventional PCC loading.
26572417	0	14	from	nanofibrils	52:62	arg1	properties					90:99	paper properties	84:99	paper properties	84:99	Effect of precipitated calcium carbonate--Cellulose nanofibrils composite filler on paper properties.
26572417	2	15	used	utilized	299:306	arg2	nanofibrils					282:292	cellulose nanofibrils	272:292	cellulose nanofibrils	272:292	In this study, cellulose nanofibrils were utilized in two different ways: a PCC-CNF composite filler and a papermaking additive in sheet forming.
26572417	4	16	theme	paper	614:618	arg1	sheets					620:625	paper sheets	614:625	paper sheets	614:625	The highest filler retention was obtained by using the PCC-CNF composite filler in paper sheets.
26572417	1	17	theme	new	104:106	arg1	concept					108:114	A new concept	102:114	A new concept of composite filler	102:134	A new concept of composite filler was developed by using cellulose nanofibrils (CNF), precipitated calcium carbonate (PCC) and cationic starch (C-starch).
26572417	2	18	theme	composite	341:349	arg1	filler					351:356	a PCC-CNF composite filler	331:356	a PCC-CNF composite filler	331:356	In this study, cellulose nanofibrils were utilized in two different ways: a PCC-CNF composite filler and a papermaking additive in sheet forming.
26572417	2	18	theme	composite	341:349	arg1	ways					325:328	two different ways	311:328	two different ways: a PCC-CNF composite filler and a papermaking additive in sheet forming	311:400	In this study, cellulose nanofibrils were utilized in two different ways: a PCC-CNF composite filler and a papermaking additive in sheet forming.
26572417	0	19	theme	calcium	23:29	arg1	carbonate--Cellulose					31:50	precipitated calcium carbonate--Cellulose	10:50	precipitated calcium carbonate--Cellulose	10:50	Effect of precipitated calcium carbonate--Cellulose nanofibrils composite filler on paper properties.
26572417	5	20	contain	had	672:674	arg1	paper					632:636	The paper	628:636	The paper filled with the composite fillers	628:670	The paper filled with the composite fillers had much higher bursting and tensile strengths than conventional PCC loading.
26572417	5	20	contain	had	672:674	arg2	strengths					709:717	tensile strengths	701:717	tensile strengths	701:717	The paper filled with the composite fillers had much higher bursting and tensile strengths than conventional PCC loading.
26572417	5	20	contain	had	672:674	arg2	bursting					688:695	much higher bursting	676:695	much higher bursting	676:695	The paper filled with the composite fillers had much higher bursting and tensile strengths than conventional PCC loading.
26572417	5	21	theme	composite	654:662	arg1	fillers					664:670	the composite fillers	650:670	the composite fillers	650:670	The paper filled with the composite fillers had much higher bursting and tensile strengths than conventional PCC loading.
26572417	2	22	theme	cellulose	272:280	arg1	nanofibrils					282:292	cellulose nanofibrils	272:292	cellulose nanofibrils	272:292	In this study, cellulose nanofibrils were utilized in two different ways: a PCC-CNF composite filler and a papermaking additive in sheet forming.
26572417	4	23	from	filler	604:609	arg1	sheets					620:625	paper sheets	614:625	paper sheets	614:625	The highest filler retention was obtained by using the PCC-CNF composite filler in paper sheets.
26572417	1	24	theme	precipitated	188:199	arg1	PCC					220:222	PCC	220:222	PCC	220:222	A new concept of composite filler was developed by using cellulose nanofibrils (CNF), precipitated calcium carbonate (PCC) and cationic starch (C-starch).
26572417	1	24	theme	precipitated	188:199	arg1	carbonate					209:217	precipitated calcium carbonate	188:217	precipitated calcium carbonate (PCC)	188:223	A new concept of composite filler was developed by using cellulose nanofibrils (CNF), precipitated calcium carbonate (PCC) and cationic starch (C-starch).
26572417	2	25	theme	forming	394:400	arg1	sheet					388:392	sheet forming	388:400	sheet forming	388:400	In this study, cellulose nanofibrils were utilized in two different ways: a PCC-CNF composite filler and a papermaking additive in sheet forming.
26572417	3	26	theme	handsheets	519:528	arg1	flocculation					445:456	flocculation	445:456	flocculation	445:456	The aim was to elucidate their effects on flocculation, filler retention and the strength and optical properties of handsheets.
26572417	3	26	theme	handsheets	519:528	arg1	retention					466:474	filler retention	459:474	filler retention	459:474	The aim was to elucidate their effects on flocculation, filler retention and the strength and optical properties of handsheets.
26572417	3	26	theme	handsheets	519:528	arg1	strength					484:491	strength	484:491	strength	484:491	The aim was to elucidate their effects on flocculation, filler retention and the strength and optical properties of handsheets.
26572417	3	26	theme	handsheets	519:528	arg1	properties					505:514	optical properties	497:514	optical properties	497:514	The aim was to elucidate their effects on flocculation, filler retention and the strength and optical properties of handsheets.
26572417	1	27	theme	calcium	201:207	arg1	PCC					220:222	PCC	220:222	PCC	220:222	A new concept of composite filler was developed by using cellulose nanofibrils (CNF), precipitated calcium carbonate (PCC) and cationic starch (C-starch).
26572417	1	27	theme	calcium	201:207	arg1	carbonate					209:217	precipitated calcium carbonate	188:217	precipitated calcium carbonate (PCC)	188:223	A new concept of composite filler was developed by using cellulose nanofibrils (CNF), precipitated calcium carbonate (PCC) and cationic starch (C-starch).
26572417	3	28	theme	filler	459:464	arg1	retention					466:474	filler retention	459:474	filler retention	459:474	The aim was to elucidate their effects on flocculation, filler retention and the strength and optical properties of handsheets.
26572417	2	29	theme	additive	376:383	arg1	ways					325:328	two different ways	311:328	two different ways: a PCC-CNF composite filler and a papermaking additive in sheet forming	311:400	In this study, cellulose nanofibrils were utilized in two different ways: a PCC-CNF composite filler and a papermaking additive in sheet forming.
26572417	2	29	theme	additive	376:383	arg1	papermaking					364:374	a papermaking	362:374	a papermaking additive in sheet forming	362:400	In this study, cellulose nanofibrils were utilized in two different ways: a PCC-CNF composite filler and a papermaking additive in sheet forming.
26572417	0	30	theme	carbonate--Cellulose	31:50	arg1	Effect					0:5	Effect	0:5	Effect of precipitated calcium carbonate--Cellulose	0:50	Effect of precipitated calcium carbonate--Cellulose nanofibrils composite filler on paper properties.
26572417	6	31	theme	composite	805:813	arg1	fillers					815:821	PCC-CNF composite fillers	797:821	PCC-CNF composite fillers	797:821	It was also found that the paper prepared with PCC-CNF composite fillers became denser with increasing the filler content of paper.
26572417	1	32	theme	composite	119:127	arg1	filler					129:134	composite filler	119:134	composite filler	119:134	A new concept of composite filler was developed by using cellulose nanofibrils (CNF), precipitated calcium carbonate (PCC) and cationic starch (C-starch).
26572417	0	33	theme	composite	64:72	arg1	filler					74:79	composite filler	64:79	composite filler	64:79	Effect of precipitated calcium carbonate--Cellulose nanofibrils composite filler on paper properties.
26572417	6	34	theme	PCC-CNF	797:803	arg1	fillers					815:821	PCC-CNF composite fillers	797:821	PCC-CNF composite fillers	797:821	It was also found that the paper prepared with PCC-CNF composite fillers became denser with increasing the filler content of paper.
26572417	1	35	theme	filler	129:134	arg1	concept					108:114	A new concept	102:114	A new concept of composite filler	102:134	A new concept of composite filler was developed by using cellulose nanofibrils (CNF), precipitated calcium carbonate (PCC) and cationic starch (C-starch).
26572417	3	36	from	effects	434:440	arg1	flocculation					445:456	flocculation	445:456	flocculation	445:456	The aim was to elucidate their effects on flocculation, filler retention and the strength and optical properties of handsheets.
26572417	3	36	from	effects	434:440	arg1	retention					466:474	filler retention	459:474	filler retention	459:474	The aim was to elucidate their effects on flocculation, filler retention and the strength and optical properties of handsheets.
26572417	3	36	from	effects	434:440	arg1	strength					484:491	strength	484:491	strength	484:491	The aim was to elucidate their effects on flocculation, filler retention and the strength and optical properties of handsheets.
26572417	2	37	from	additive	376:383	arg1	sheet					388:392	sheet forming	388:400	sheet forming	388:400	In this study, cellulose nanofibrils were utilized in two different ways: a PCC-CNF composite filler and a papermaking additive in sheet forming.
26572417	0	38	theme	paper	84:88	arg1	properties					90:99	paper properties	84:99	paper properties	84:99	Effect of precipitated calcium carbonate--Cellulose nanofibrils composite filler on paper properties.
26572417	4	39	theme	composite	594:602	arg1	filler					604:609	the PCC-CNF composite filler	582:609	the PCC-CNF composite filler in paper sheets	582:625	The highest filler retention was obtained by using the PCC-CNF composite filler in paper sheets.
26572417	2	40	theme	PCC-CNF	333:339	arg1	filler					351:356	a PCC-CNF composite filler	331:356	a PCC-CNF composite filler	331:356	In this study, cellulose nanofibrils were utilized in two different ways: a PCC-CNF composite filler and a papermaking additive in sheet forming.
26572417	2	40	theme	PCC-CNF	333:339	arg1	ways					325:328	two different ways	311:328	two different ways: a PCC-CNF composite filler and a papermaking additive in sheet forming	311:400	In this study, cellulose nanofibrils were utilized in two different ways: a PCC-CNF composite filler and a papermaking additive in sheet forming.
26572417	0	41	theme	precipitated	10:21	arg1	carbonate--Cellulose					31:50	precipitated calcium carbonate--Cellulose	10:50	precipitated calcium carbonate--Cellulose	10:50	Effect of precipitated calcium carbonate--Cellulose nanofibrils composite filler on paper properties.
26572417	2	42	from	sheet	388:392	arg1	additive					376:383	additive	376:383	additive	376:383	In this study, cellulose nanofibrils were utilized in two different ways: a PCC-CNF composite filler and a papermaking additive in sheet forming.
26572417	1	43	theme	cationic	229:236	arg1	C-starch					246:253	C-starch	246:253	C-starch	246:253	A new concept of composite filler was developed by using cellulose nanofibrils (CNF), precipitated calcium carbonate (PCC) and cationic starch (C-starch).
26572417	1	43	theme	cationic	229:236	arg1	starch					238:243	cationic starch	229:243	cationic starch (C-starch)	229:254	A new concept of composite filler was developed by using cellulose nanofibrils (CNF), precipitated calcium carbonate (PCC) and cationic starch (C-starch).
25124516	6	0	from	patients	1059:1066	arg1	region					1004:1009	the Fc region	997:1009	the Fc region of total IgG from Wuchereria bancrofti-infected patients characterizing the two clinical manifestations	997:1113	We purified N-linked glycans from the Fc region of total IgG from Wuchereria bancrofti-infected patients characterizing the two clinical manifestations (chronic pathology and asymptomatic infection) and compared them to infection-free endemic normals.
25124516	6	0	from	patients	1059:1066	arg1	IgG					1020:1022	total IgG	1014:1022	total IgG from Wuchereria bancrofti-infected patients characterizing the two clinical manifestations	1014:1113	We purified N-linked glycans from the Fc region of total IgG from Wuchereria bancrofti-infected patients characterizing the two clinical manifestations (chronic pathology and asymptomatic infection) and compared them to infection-free endemic normals.
25124516	6	1	theme	bancrofti-infected	1040:1057	arg1	patients					1059:1066	Wuchereria bancrofti-infected patients	1029:1066	Wuchereria bancrofti-infected patients characterizing the two clinical manifestations	1029:1113	We purified N-linked glycans from the Fc region of total IgG from Wuchereria bancrofti-infected patients characterizing the two clinical manifestations (chronic pathology and asymptomatic infection) and compared them to infection-free endemic normals.
25124516	7	2	contain	had	1388:1390	arg1	patients					1379:1386	asymptomatically infected patients	1353:1386	asymptomatically infected patients	1353:1386	Using capillary electrophoresis, we found that there was no difference in galactosylation of total IgG between the three groups; however, asymptomatically infected patients had significantly lower levels of disialylated IgG compared to endemic normals and patients with pathology.
25124516	7	2	contain	had	1388:1390	arg2	levels					1412:1417	significantly lower levels	1392:1417	significantly lower levels of disialylated IgG	1392:1437	Using capillary electrophoresis, we found that there was no difference in galactosylation of total IgG between the three groups; however, asymptomatically infected patients had significantly lower levels of disialylated IgG compared to endemic normals and patients with pathology.
25124516	1	3	theme	2-type	159:164	arg1	responses					166:174	strong regulatory and T helper 2-type responses	128:174	strong regulatory and T helper 2-type responses	128:174	Helminths induce strong regulatory and T helper 2-type responses, whereby antibody-derived host protection and regulation are essential components.
25124516	6	4	theme	IgG	1020:1022	arg1	region					1004:1009	the Fc region	997:1009	the Fc region of total IgG from Wuchereria bancrofti-infected patients characterizing the two clinical manifestations	997:1113	We purified N-linked glycans from the Fc region of total IgG from Wuchereria bancrofti-infected patients characterizing the two clinical manifestations (chronic pathology and asymptomatic infection) and compared them to infection-free endemic normals.
25124516	4	5	theme	post-translational	631:648	arg1	modification					650:661	a post-translational modification	629:661	a post-translational modification required for the structure and molecular function, influencing host inflammatory and regulatory responses	629:767	N-glycosylation of the Fc region of antibodies is a post-translational modification required for the structure and molecular function, influencing host inflammatory and regulatory responses.
25124516	4	5	theme	post-translational	631:648	arg1	N-glycosylation					579:593	N-glycosylation	579:593	N-glycosylation of the Fc region of antibodies	579:624	N-glycosylation of the Fc region of antibodies is a post-translational modification required for the structure and molecular function, influencing host inflammatory and regulatory responses.
25124516	7	6	theme	infected	1370:1377	arg1	patients					1379:1386	asymptomatically infected patients	1353:1386	asymptomatically infected patients	1353:1386	Using capillary electrophoresis, we found that there was no difference in galactosylation of total IgG between the three groups; however, asymptomatically infected patients had significantly lower levels of disialylated IgG compared to endemic normals and patients with pathology.
25124516	6	7	theme	Wuchereria	1029:1038	arg1	patients					1059:1066	Wuchereria bancrofti-infected patients	1029:1066	Wuchereria bancrofti-infected patients characterizing the two clinical manifestations	1029:1113	We purified N-linked glycans from the Fc region of total IgG from Wuchereria bancrofti-infected patients characterizing the two clinical manifestations (chronic pathology and asymptomatic infection) and compared them to infection-free endemic normals.
25124516	0	8	gly	disialylated	65:76	arg1	IgG					78:80	disialylated IgG	65:80	disialylated IgG	65:80	Filariasis asymptomatically infected donors have lower levels of disialylated IgG compared to endemic normals.
25124516	6	9	theme	asymptomatic	1138:1149	arg1	infection					1151:1159	asymptomatic infection	1138:1159	asymptomatic infection	1138:1159	We purified N-linked glycans from the Fc region of total IgG from Wuchereria bancrofti-infected patients characterizing the two clinical manifestations (chronic pathology and asymptomatic infection) and compared them to infection-free endemic normals.
25124516	0	10	theme	endemic	94:100	arg1	normals					102:108	endemic normals	94:108	endemic normals	94:108	Filariasis asymptomatically infected donors have lower levels of disialylated IgG compared to endemic normals.
25124516	6	11	link	N-linked	975:982	arg1	glycans					984:990	N-linked glycans	975:990	N-linked glycans	975:990	We purified N-linked glycans from the Fc region of total IgG from Wuchereria bancrofti-infected patients characterizing the two clinical manifestations (chronic pathology and asymptomatic infection) and compared them to infection-free endemic normals.
25124516	0	12	contain	have	44:47	arg2	levels					55:60	lower levels	49:60	lower levels of disialylated IgG	49:80	Filariasis asymptomatically infected donors have lower levels of disialylated IgG compared to endemic normals.
25124516	0	12	contain	have	44:47	arg1	donors					37:42	Filariasis asymptomatically infected donors	0:42	Filariasis asymptomatically infected donors	0:42	Filariasis asymptomatically infected donors have lower levels of disialylated IgG compared to endemic normals.
25124516	2	13	theme	asymptomatic	386:397	arg1	outcomes					355:362	two main clinical outcomes	337:362	two main clinical outcomes: chronic pathology or asymptomatic infection	337:407	Lymphatic filariasis is an immune-mediated spectral disease that manifests as two main clinical outcomes: chronic pathology or asymptomatic infection.
25124516	2	13	theme	asymptomatic	386:397	arg1	infection					399:407	asymptomatic infection	386:407	asymptomatic infection	386:407	Lymphatic filariasis is an immune-mediated spectral disease that manifests as two main clinical outcomes: chronic pathology or asymptomatic infection.
25124516	5	14	theme	decreased	829:837	arg1	galactosylation					839:853	decreased galactosylation	829:853	decreased galactosylation	829:853	Altered IgG glycosylation correlates with disease, whereby decreased galactosylation is associated with inflammation while increased sialylation is associated with anti-inflammatory responses.
25124516	6	15	theme	total	1014:1018	arg1	IgG					1020:1022	total IgG	1014:1022	total IgG from Wuchereria bancrofti-infected patients characterizing the two clinical manifestations	1014:1113	We purified N-linked glycans from the Fc region of total IgG from Wuchereria bancrofti-infected patients characterizing the two clinical manifestations (chronic pathology and asymptomatic infection) and compared them to infection-free endemic normals.
25124516	1	16	link	antibody-derived	185:200	arg1	regulation					222:231	regulation	222:231	regulation	222:231	Helminths induce strong regulatory and T helper 2-type responses, whereby antibody-derived host protection and regulation are essential components.
25124516	1	16	link	antibody-derived	185:200	arg1	components					247:256	essential components	237:256	essential components	237:256	Helminths induce strong regulatory and T helper 2-type responses, whereby antibody-derived host protection and regulation are essential components.
25124516	1	16	link	antibody-derived	185:200	arg1	protection					207:216	antibody-derived host protection	185:216	antibody-derived host protection	185:216	Helminths induce strong regulatory and T helper 2-type responses, whereby antibody-derived host protection and regulation are essential components.
25124516	4	17	theme	regulatory	748:757	arg1	responses					759:767	host inflammatory and regulatory responses	726:767	responses	759:767	N-glycosylation of the Fc region of antibodies is a post-translational modification required for the structure and molecular function, influencing host inflammatory and regulatory responses.
25124516	3	18	theme	parasite-induced	469:484	arg1	immunoregulation					486:501	parasite-induced immunoregulation	469:501	parasite-induced immunoregulation	469:501	These outcomes depend on a multitude of factors, including parasite-induced immunoregulation and host genetic background; antibody responses contribute to this outcome.
25124516	7	19	theme	total	1308:1312	arg1	IgG					1314:1316	total IgG	1308:1316	total IgG	1308:1316	Using capillary electrophoresis, we found that there was no difference in galactosylation of total IgG between the three groups; however, asymptomatically infected patients had significantly lower levels of disialylated IgG compared to endemic normals and patients with pathology.
25124516	2	20	theme	spectral	302:309	arg1	disease					311:317	an immune-mediated spectral disease	283:317	an immune-mediated spectral disease that manifests as two main clinical outcomes: chronic pathology or asymptomatic infection	283:407	Lymphatic filariasis is an immune-mediated spectral disease that manifests as two main clinical outcomes: chronic pathology or asymptomatic infection.
25124516	2	20	theme	spectral	302:309	arg1	filariasis					269:278	Lymphatic filariasis	259:278	Lymphatic filariasis	259:278	Lymphatic filariasis is an immune-mediated spectral disease that manifests as two main clinical outcomes: chronic pathology or asymptomatic infection.
25124516	7	21	from	difference	1275:1284	arg1	galactosylation					1289:1303	galactosylation	1289:1303	galactosylation of total IgG	1289:1316	Using capillary electrophoresis, we found that there was no difference in galactosylation of total IgG between the three groups; however, asymptomatically infected patients had significantly lower levels of disialylated IgG compared to endemic normals and patients with pathology.
25124516	4	22	theme	antibodies	615:624	arg1	region					605:610	the Fc region	598:610	the Fc region of antibodies	598:624	N-glycosylation of the Fc region of antibodies is a post-translational modification required for the structure and molecular function, influencing host inflammatory and regulatory responses.
25124516	2	23	theme	clinical	346:353	arg1	outcomes					355:362	two main clinical outcomes	337:362	two main clinical outcomes: chronic pathology or asymptomatic infection	337:407	Lymphatic filariasis is an immune-mediated spectral disease that manifests as two main clinical outcomes: chronic pathology or asymptomatic infection.
25124516	2	23	theme	clinical	346:353	arg1	pathology					373:381	chronic pathology	365:381	chronic pathology	365:381	Lymphatic filariasis is an immune-mediated spectral disease that manifests as two main clinical outcomes: chronic pathology or asymptomatic infection.
25124516	2	23	theme	clinical	346:353	arg1	infection					399:407	asymptomatic infection	386:407	asymptomatic infection	386:407	Lymphatic filariasis is an immune-mediated spectral disease that manifests as two main clinical outcomes: chronic pathology or asymptomatic infection.
25124516	1	24	theme	antibody-derived	185:200	arg1	regulation					222:231	regulation	222:231	regulation	222:231	Helminths induce strong regulatory and T helper 2-type responses, whereby antibody-derived host protection and regulation are essential components.
25124516	1	24	theme	antibody-derived	185:200	arg1	components					247:256	essential components	237:256	essential components	237:256	Helminths induce strong regulatory and T helper 2-type responses, whereby antibody-derived host protection and regulation are essential components.
25124516	1	24	theme	antibody-derived	185:200	arg1	protection					207:216	antibody-derived host protection	185:216	antibody-derived host protection	185:216	Helminths induce strong regulatory and T helper 2-type responses, whereby antibody-derived host protection and regulation are essential components.
25124516	0	25	theme	Filariasis	0:9	arg1	donors					37:42	Filariasis asymptomatically infected donors	0:42	Filariasis asymptomatically infected donors	0:42	Filariasis asymptomatically infected donors have lower levels of disialylated IgG compared to endemic normals.
25124516	7	26	theme	IgG	1314:1316	arg1	galactosylation					1289:1303	galactosylation	1289:1303	galactosylation of total IgG	1289:1316	Using capillary electrophoresis, we found that there was no difference in galactosylation of total IgG between the three groups; however, asymptomatically infected patients had significantly lower levels of disialylated IgG compared to endemic normals and patients with pathology.
25124516	2	27	theme	main	341:344	arg1	outcomes					355:362	two main clinical outcomes	337:362	two main clinical outcomes: chronic pathology or asymptomatic infection	337:407	Lymphatic filariasis is an immune-mediated spectral disease that manifests as two main clinical outcomes: chronic pathology or asymptomatic infection.
25124516	2	27	theme	main	341:344	arg1	pathology					373:381	chronic pathology	365:381	chronic pathology	365:381	Lymphatic filariasis is an immune-mediated spectral disease that manifests as two main clinical outcomes: chronic pathology or asymptomatic infection.
25124516	2	27	theme	main	341:344	arg1	infection					399:407	asymptomatic infection	386:407	asymptomatic infection	386:407	Lymphatic filariasis is an immune-mediated spectral disease that manifests as two main clinical outcomes: chronic pathology or asymptomatic infection.
25124516	1	28	theme	host	202:205	arg1	regulation					222:231	regulation	222:231	regulation	222:231	Helminths induce strong regulatory and T helper 2-type responses, whereby antibody-derived host protection and regulation are essential components.
25124516	1	28	theme	host	202:205	arg1	components					247:256	essential components	237:256	essential components	237:256	Helminths induce strong regulatory and T helper 2-type responses, whereby antibody-derived host protection and regulation are essential components.
25124516	1	28	theme	host	202:205	arg1	protection					207:216	antibody-derived host protection	185:216	antibody-derived host protection	185:216	Helminths induce strong regulatory and T helper 2-type responses, whereby antibody-derived host protection and regulation are essential components.
25124516	5	29	theme	anti-inflammatory	934:950	arg1	responses					952:960	anti-inflammatory responses	934:960	anti-inflammatory responses	934:960	Altered IgG glycosylation correlates with disease, whereby decreased galactosylation is associated with inflammation while increased sialylation is associated with anti-inflammatory responses.
25124516	4	30	theme	region	605:610	arg1	modification					650:661	a post-translational modification	629:661	a post-translational modification required for the structure and molecular function, influencing host inflammatory and regulatory responses	629:767	N-glycosylation of the Fc region of antibodies is a post-translational modification required for the structure and molecular function, influencing host inflammatory and regulatory responses.
25124516	4	30	theme	region	605:610	arg1	N-glycosylation					579:593	N-glycosylation	579:593	N-glycosylation of the Fc region of antibodies	579:624	N-glycosylation of the Fc region of antibodies is a post-translational modification required for the structure and molecular function, influencing host inflammatory and regulatory responses.
25124516	7	31	gly	disialylated	1422:1433	arg1	IgG					1435:1437	disialylated IgG	1422:1437	disialylated IgG	1422:1437	Using capillary electrophoresis, we found that there was no difference in galactosylation of total IgG between the three groups; however, asymptomatically infected patients had significantly lower levels of disialylated IgG compared to endemic normals and patients with pathology.
25124516	3	32	theme	genetic	512:518	arg1	background					520:529	host genetic background	507:529	host genetic background	507:529	These outcomes depend on a multitude of factors, including parasite-induced immunoregulation and host genetic background; antibody responses contribute to this outcome.
25124516	0	33	theme	infected	28:35	arg1	donors					37:42	Filariasis asymptomatically infected donors	0:42	Filariasis asymptomatically infected donors	0:42	Filariasis asymptomatically infected donors have lower levels of disialylated IgG compared to endemic normals.
25124516	6	34	theme	Fc	1001:1002	arg1	region					1004:1009	the Fc region	997:1009	the Fc region of total IgG from Wuchereria bancrofti-infected patients characterizing the two clinical manifestations	997:1113	We purified N-linked glycans from the Fc region of total IgG from Wuchereria bancrofti-infected patients characterizing the two clinical manifestations (chronic pathology and asymptomatic infection) and compared them to infection-free endemic normals.
25124516	6	35	dep	purified	966:973	arg1	infection					1151:1159	asymptomatic infection	1138:1159	asymptomatic infection	1138:1159	We purified N-linked glycans from the Fc region of total IgG from Wuchereria bancrofti-infected patients characterizing the two clinical manifestations (chronic pathology and asymptomatic infection) and compared them to infection-free endemic normals.
25124516	6	35	dep	purified	966:973	arg1	pathology					1124:1132	chronic pathology	1116:1132	chronic pathology	1116:1132	We purified N-linked glycans from the Fc region of total IgG from Wuchereria bancrofti-infected patients characterizing the two clinical manifestations (chronic pathology and asymptomatic infection) and compared them to infection-free endemic normals.
25124516	4	36	theme	molecular	694:702	arg1	function					704:711	molecular function	694:711	molecular function	694:711	N-glycosylation of the Fc region of antibodies is a post-translational modification required for the structure and molecular function, influencing host inflammatory and regulatory responses.
25124516	0	37	theme	lower	49:53	arg1	levels					55:60	lower levels	49:60	lower levels of disialylated IgG	49:80	Filariasis asymptomatically infected donors have lower levels of disialylated IgG compared to endemic normals.
25124516	2	38	theme	chronic	365:371	arg1	outcomes					355:362	two main clinical outcomes	337:362	two main clinical outcomes: chronic pathology or asymptomatic infection	337:407	Lymphatic filariasis is an immune-mediated spectral disease that manifests as two main clinical outcomes: chronic pathology or asymptomatic infection.
25124516	2	38	theme	chronic	365:371	arg1	pathology					373:381	chronic pathology	365:381	chronic pathology	365:381	Lymphatic filariasis is an immune-mediated spectral disease that manifests as two main clinical outcomes: chronic pathology or asymptomatic infection.
25124516	7	39	with	patients	1471:1478	arg1	pathology					1485:1493	pathology	1485:1493	pathology	1485:1493	Using capillary electrophoresis, we found that there was no difference in galactosylation of total IgG between the three groups; however, asymptomatically infected patients had significantly lower levels of disialylated IgG compared to endemic normals and patients with pathology.
25124516	2	40	dep	outcomes	355:362	arg1	outcomes					355:362	two main clinical outcomes	337:362	two main clinical outcomes: chronic pathology or asymptomatic infection	337:407	Lymphatic filariasis is an immune-mediated spectral disease that manifests as two main clinical outcomes: chronic pathology or asymptomatic infection.
25124516	2	40	dep	outcomes	355:362	arg1	pathology					373:381	chronic pathology	365:381	chronic pathology	365:381	Lymphatic filariasis is an immune-mediated spectral disease that manifests as two main clinical outcomes: chronic pathology or asymptomatic infection.
25124516	2	40	dep	outcomes	355:362	arg1	infection					399:407	asymptomatic infection	386:407	asymptomatic infection	386:407	Lymphatic filariasis is an immune-mediated spectral disease that manifests as two main clinical outcomes: chronic pathology or asymptomatic infection.
25124516	2	41	theme	Lymphatic	259:267	arg1	disease					311:317	an immune-mediated spectral disease	283:317	an immune-mediated spectral disease that manifests as two main clinical outcomes: chronic pathology or asymptomatic infection	283:407	Lymphatic filariasis is an immune-mediated spectral disease that manifests as two main clinical outcomes: chronic pathology or asymptomatic infection.
25124516	2	41	theme	Lymphatic	259:267	arg1	filariasis					269:278	Lymphatic filariasis	259:278	Lymphatic filariasis	259:278	Lymphatic filariasis is an immune-mediated spectral disease that manifests as two main clinical outcomes: chronic pathology or asymptomatic infection.
25124516	6	42	theme	endemic	1198:1204	arg1	normals					1206:1212	infection-free endemic normals	1183:1212	infection-free endemic normals	1183:1212	We purified N-linked glycans from the Fc region of total IgG from Wuchereria bancrofti-infected patients characterizing the two clinical manifestations (chronic pathology and asymptomatic infection) and compared them to infection-free endemic normals.
25124516	3	43	theme	factors	450:456	arg1	multitude					437:445	a multitude	435:445	a multitude of factors, including parasite-induced immunoregulation and host genetic background	435:529	These outcomes depend on a multitude of factors, including parasite-induced immunoregulation and host genetic background; antibody responses contribute to this outcome.
25124516	6	44	theme	clinical	1091:1098	arg1	manifestations					1100:1113	the two clinical manifestations	1083:1113	the two clinical manifestations	1083:1113	We purified N-linked glycans from the Fc region of total IgG from Wuchereria bancrofti-infected patients characterizing the two clinical manifestations (chronic pathology and asymptomatic infection) and compared them to infection-free endemic normals.
25124516	5	45	theme	increased	893:901	arg1	sialylation					903:913	increased sialylation	893:913	increased sialylation	893:913	Altered IgG glycosylation correlates with disease, whereby decreased galactosylation is associated with inflammation while increased sialylation is associated with anti-inflammatory responses.
25124516	8	46	theme	disease	1565:1571	arg1	outcome					1573:1579	disease outcome	1565:1579	disease outcome	1565:1579	These data suggest that while galactosylation does not contribute to disease outcome, sialylation may be involved in asymptomatic infection.
25124516	3	47	theme	host	507:510	arg1	background					520:529	host genetic background	507:529	host genetic background	507:529	These outcomes depend on a multitude of factors, including parasite-induced immunoregulation and host genetic background; antibody responses contribute to this outcome.
25124516	6	48	theme	chronic	1116:1122	arg1	pathology					1124:1132	chronic pathology	1116:1132	chronic pathology	1116:1132	We purified N-linked glycans from the Fc region of total IgG from Wuchereria bancrofti-infected patients characterizing the two clinical manifestations (chronic pathology and asymptomatic infection) and compared them to infection-free endemic normals.
25124516	7	49	theme	capillary	1221:1229	arg1	electrophoresis					1231:1245	capillary electrophoresis	1221:1245	capillary electrophoresis	1221:1245	Using capillary electrophoresis, we found that there was no difference in galactosylation of total IgG between the three groups; however, asymptomatically infected patients had significantly lower levels of disialylated IgG compared to endemic normals and patients with pathology.
25124516	4	50	theme	Fc	602:603	arg1	region					605:610	the Fc region	598:610	the Fc region of antibodies	598:624	N-glycosylation of the Fc region of antibodies is a post-translational modification required for the structure and molecular function, influencing host inflammatory and regulatory responses.
25124516	7	51	with	normals	1459:1465	arg1	pathology					1485:1493	pathology	1485:1493	pathology	1485:1493	Using capillary electrophoresis, we found that there was no difference in galactosylation of total IgG between the three groups; however, asymptomatically infected patients had significantly lower levels of disialylated IgG compared to endemic normals and patients with pathology.
25124516	6	52	theme	N-linked	975:982	arg1	glycans					984:990	N-linked glycans	975:990	N-linked glycans	975:990	We purified N-linked glycans from the Fc region of total IgG from Wuchereria bancrofti-infected patients characterizing the two clinical manifestations (chronic pathology and asymptomatic infection) and compared them to infection-free endemic normals.
25124516	1	53	theme	strong	128:133	arg1	responses					166:174	strong regulatory and T helper 2-type responses	128:174	strong regulatory and T helper 2-type responses	128:174	Helminths induce strong regulatory and T helper 2-type responses, whereby antibody-derived host protection and regulation are essential components.
25124516	8	54	theme	asymptomatic	1613:1624	arg1	infection					1626:1634	asymptomatic infection	1613:1634	asymptomatic infection	1613:1634	These data suggest that while galactosylation does not contribute to disease outcome, sialylation may be involved in asymptomatic infection.
25124516	5	55	theme	Altered	770:776	arg1	glycosylation					782:794	Altered IgG glycosylation	770:794	Altered IgG glycosylation	770:794	Altered IgG glycosylation correlates with disease, whereby decreased galactosylation is associated with inflammation while increased sialylation is associated with anti-inflammatory responses.
25124516	4	56	dep	structure	680:688	arg1	the					676:678	the	676:678	the	676:678	N-glycosylation of the Fc region of antibodies is a post-translational modification required for the structure and molecular function, influencing host inflammatory and regulatory responses.
25124516	2	57	theme	immune-mediated	286:300	arg1	disease					311:317	an immune-mediated spectral disease	283:317	an immune-mediated spectral disease that manifests as two main clinical outcomes: chronic pathology or asymptomatic infection	283:407	Lymphatic filariasis is an immune-mediated spectral disease that manifests as two main clinical outcomes: chronic pathology or asymptomatic infection.
25124516	2	57	theme	immune-mediated	286:300	arg1	filariasis					269:278	Lymphatic filariasis	259:278	Lymphatic filariasis	259:278	Lymphatic filariasis is an immune-mediated spectral disease that manifests as two main clinical outcomes: chronic pathology or asymptomatic infection.
25124516	8	58	gly	sialylation	1582:1592	arg1	infection					1626:1634	asymptomatic infection	1613:1634	asymptomatic infection	1613:1634	These data suggest that while galactosylation does not contribute to disease outcome, sialylation may be involved in asymptomatic infection.
25124516	1	59	theme	regulatory	135:144	arg1	responses					166:174	strong regulatory and T helper 2-type responses	128:174	strong regulatory and T helper 2-type responses	128:174	Helminths induce strong regulatory and T helper 2-type responses, whereby antibody-derived host protection and regulation are essential components.
25124516	1	60	theme	essential	237:245	arg1	regulation					222:231	regulation	222:231	regulation	222:231	Helminths induce strong regulatory and T helper 2-type responses, whereby antibody-derived host protection and regulation are essential components.
25124516	1	60	theme	essential	237:245	arg1	components					247:256	essential components	237:256	essential components	237:256	Helminths induce strong regulatory and T helper 2-type responses, whereby antibody-derived host protection and regulation are essential components.
25124516	1	60	theme	essential	237:245	arg1	protection					207:216	antibody-derived host protection	185:216	antibody-derived host protection	185:216	Helminths induce strong regulatory and T helper 2-type responses, whereby antibody-derived host protection and regulation are essential components.
25124516	5	61	theme	IgG	778:780	arg1	glycosylation					782:794	Altered IgG glycosylation	770:794	Altered IgG glycosylation	770:794	Altered IgG glycosylation correlates with disease, whereby decreased galactosylation is associated with inflammation while increased sialylation is associated with anti-inflammatory responses.
25124516	4	62	gly	N-glycosylation	579:593	arg1	region					605:610	the Fc region	598:610	the Fc region of antibodies	598:624	N-glycosylation of the Fc region of antibodies is a post-translational modification required for the structure and molecular function, influencing host inflammatory and regulatory responses.
25124516	3	63	theme	antibody	532:539	arg1	responses					541:549	antibody responses	532:549	antibody responses	532:549	These outcomes depend on a multitude of factors, including parasite-induced immunoregulation and host genetic background; antibody responses contribute to this outcome.
25124516	7	64	theme	IgG	1435:1437	arg1	levels					1412:1417	significantly lower levels	1392:1417	significantly lower levels of disialylated IgG	1392:1437	Using capillary electrophoresis, we found that there was no difference in galactosylation of total IgG between the three groups; however, asymptomatically infected patients had significantly lower levels of disialylated IgG compared to endemic normals and patients with pathology.
25124516	0	65	theme	IgG	78:80	arg1	levels					55:60	lower levels	49:60	lower levels of disialylated IgG	49:80	Filariasis asymptomatically infected donors have lower levels of disialylated IgG compared to endemic normals.
25124516	6	66	theme	infection-free	1183:1196	arg1	normals					1206:1212	infection-free endemic normals	1183:1212	infection-free endemic normals	1183:1212	We purified N-linked glycans from the Fc region of total IgG from Wuchereria bancrofti-infected patients characterizing the two clinical manifestations (chronic pathology and asymptomatic infection) and compared them to infection-free endemic normals.
25124516	0	67	theme	disialylated	65:76	arg1	IgG					78:80	disialylated IgG	65:80	disialylated IgG	65:80	Filariasis asymptomatically infected donors have lower levels of disialylated IgG compared to endemic normals.
25124516	7	68	theme	endemic	1451:1457	arg1	normals					1459:1465	endemic normals	1451:1465	endemic normals	1451:1465	Using capillary electrophoresis, we found that there was no difference in galactosylation of total IgG between the three groups; however, asymptomatically infected patients had significantly lower levels of disialylated IgG compared to endemic normals and patients with pathology.
25124516	7	69	theme	disialylated	1422:1433	arg1	IgG					1435:1437	disialylated IgG	1422:1437	disialylated IgG	1422:1437	Using capillary electrophoresis, we found that there was no difference in galactosylation of total IgG between the three groups; however, asymptomatically infected patients had significantly lower levels of disialylated IgG compared to endemic normals and patients with pathology.
25124516	7	70	theme	lower	1406:1410	arg1	levels					1412:1417	significantly lower levels	1392:1417	significantly lower levels of disialylated IgG	1392:1437	Using capillary electrophoresis, we found that there was no difference in galactosylation of total IgG between the three groups; however, asymptomatically infected patients had significantly lower levels of disialylated IgG compared to endemic normals and patients with pathology.
25124516	6	71	from	region	1004:1009	arg1	patients					1059:1066	Wuchereria bancrofti-infected patients	1029:1066	Wuchereria bancrofti-infected patients characterizing the two clinical manifestations	1029:1113	We purified N-linked glycans from the Fc region of total IgG from Wuchereria bancrofti-infected patients characterizing the two clinical manifestations (chronic pathology and asymptomatic infection) and compared them to infection-free endemic normals.
26826268	8	0	theme	lactic	1343:1348	arg1	LDH					1365:1367	LDH	1365:1367	LDH	1365:1367	Serum amylase (AMS) and lactic dehydrogenase (LDH) changes indicated the therapeutic effects of GLPS3.
26826268	8	0	theme	lactic	1343:1348	arg1	dehydrogenase					1350:1362	lactic dehydrogenase	1343:1362	lactic dehydrogenase (LDH)	1343:1368	Serum amylase (AMS) and lactic dehydrogenase (LDH) changes indicated the therapeutic effects of GLPS3.
26826268	8	1	theme	amylase	1325:1331	arg1	changes					1370:1376	Serum amylase (AMS) and lactic dehydrogenase (LDH) changes	1319:1376	Serum amylase (AMS) and lactic dehydrogenase (LDH) changes	1319:1376	Serum amylase (AMS) and lactic dehydrogenase (LDH) changes indicated the therapeutic effects of GLPS3.
26826268	8	2	theme	therapeutic	1392:1402	arg1	effects					1404:1410	the therapeutic effects	1388:1410	the therapeutic effects of GLPS3	1388:1419	Serum amylase (AMS) and lactic dehydrogenase (LDH) changes indicated the therapeutic effects of GLPS3.
26826268	7	3	theme	glutathione	1179:1189	arg1	GSH-Px					1203:1208	GSH-Px	1203:1208	GSH-Px	1203:1208	The superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in serum were increased while the malondialdehyde (MDA) levels were reversely decreased by GLPS3 treatment.
26826268	7	3	theme	glutathione	1179:1189	arg1	peroxidase					1191:1200	glutathione peroxidase	1179:1200	glutathione peroxidase (GSH-Px)	1179:1209	The superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in serum were increased while the malondialdehyde (MDA) levels were reversely decreased by GLPS3 treatment.
26826268	2	4	theme	diabetes	361:368	arg1	diarrhoea					305:313	diarrhoea	305:313	diarrhoea	305:313	Patients with chronic pancreatitis often present with abdominal pain, diarrhoea, jaundice, weight loss and the development of diabetes.
26826268	2	4	theme	diabetes	361:368	arg1	development					346:356	the development	342:356	the development of diabetes	342:368	Patients with chronic pancreatitis often present with abdominal pain, diarrhoea, jaundice, weight loss and the development of diabetes.
26826268	2	4	theme	diabetes	361:368	arg1	pain					299:302	abdominal pain	289:302	abdominal pain	289:302	Patients with chronic pancreatitis often present with abdominal pain, diarrhoea, jaundice, weight loss and the development of diabetes.
26826268	2	4	theme	diabetes	361:368	arg1	jaundice					316:323	jaundice	316:323	jaundice	316:323	Patients with chronic pancreatitis often present with abdominal pain, diarrhoea, jaundice, weight loss and the development of diabetes.
26826268	2	4	theme	diabetes	361:368	arg1	loss					333:336	weight loss	326:336	weight loss	326:336	Patients with chronic pancreatitis often present with abdominal pain, diarrhoea, jaundice, weight loss and the development of diabetes.
26826268	6	5	theme	mycelia	1085:1091	arg1	activity					1052:1059	The antioxidative activity	1034:1059	The antioxidative activity of GLPS3-Ⅱfrom cultured mycelia in vitro	1034:1100	The antioxidative activity of GLPS3-Ⅱfrom cultured mycelia in vitro is higher than other two polysaccharides.
26826268	6	5	theme	mycelia	1085:1091	arg1	higher					1105:1110	higher	1105:1110	higher	1105:1110	The antioxidative activity of GLPS3-Ⅱfrom cultured mycelia in vitro is higher than other two polysaccharides.
26826268	10	6	theme	CP	1617:1618	arg1	therapy					1620:1626	CP therapy	1617:1626	CP therapy	1617:1626	The results revealed that GLPS3 especially GLPS3-Ⅱfrom cultured mycelia were effective for CP therapy and bioactivity difference might be attributed to monosaccharide composition.
26826268	10	7	theme	bioactivity	1632:1642	arg1	difference					1644:1653	bioactivity difference	1632:1653	bioactivity difference	1632:1653	The results revealed that GLPS3 especially GLPS3-Ⅱfrom cultured mycelia were effective for CP therapy and bioactivity difference might be attributed to monosaccharide composition.
26826268	5	8	theme	sugar	631:635	arg1	%					752:752	90.4%	748:752	90.4%	748:752	The total sugar content of GLPS3 from fermentation broth (GLPS3-Ⅰ), cultured mycelia (GLPS3-Ⅱ) and fruiting body (GLPS3-Ⅲ) was 90.4%, 92.2% and 91.8% respectively.
26826268	5	8	theme	sugar	631:635	arg1	content					637:643	The total sugar content	621:643	The total sugar content of GLPS3 from fermentation broth (GLPS3-Ⅰ), cultured mycelia (GLPS3-Ⅱ) and fruiting body (GLPS3-Ⅲ)	621:742	The total sugar content of GLPS3 from fermentation broth (GLPS3-Ⅰ), cultured mycelia (GLPS3-Ⅱ) and fruiting body (GLPS3-Ⅲ) was 90.4%, 92.2% and 91.8% respectively.
26826268	8	9	theme	dehydrogenase	1350:1362	arg1	changes					1370:1376	Serum amylase (AMS) and lactic dehydrogenase (LDH) changes	1319:1376	Serum amylase (AMS) and lactic dehydrogenase (LDH) changes	1319:1376	Serum amylase (AMS) and lactic dehydrogenase (LDH) changes indicated the therapeutic effects of GLPS3.
26826268	6	10	theme	other	1117:1121	arg1	polysaccharides					1127:1141	other two polysaccharides	1117:1141	other two polysaccharides	1117:1141	The antioxidative activity of GLPS3-Ⅱfrom cultured mycelia in vitro is higher than other two polysaccharides.
26826268	3	11	contain	possess	426:432	arg2	activities					469:478	antioxidative and immunomodulatory activities	434:478	antioxidative and immunomodulatory activities	434:478	Polysaccharides of Ganoderma lucidum strain S3 (GLPS3) possess antioxidative and immunomodulatory activities.
26826268	3	11	contain	possess	426:432	arg1	Polysaccharides					371:385	Polysaccharides	371:385	Polysaccharides of Ganoderma lucidum strain S3 (GLPS3)	371:424	Polysaccharides of Ganoderma lucidum strain S3 (GLPS3) possess antioxidative and immunomodulatory activities.
26826268	7	12	theme	GLPS3	1302:1306	arg1	treatment					1308:1316	GLPS3 treatment	1302:1316	GLPS3 treatment	1302:1316	The superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in serum were increased while the malondialdehyde (MDA) levels were reversely decreased by GLPS3 treatment.
26826268	3	13	theme	antioxidative	434:446	arg1	activities					469:478	antioxidative and immunomodulatory activities	434:478	antioxidative and immunomodulatory activities	434:478	Polysaccharides of Ganoderma lucidum strain S3 (GLPS3) possess antioxidative and immunomodulatory activities.
26826268	3	14	theme	immunomodulatory	452:467	arg1	activities					469:478	antioxidative and immunomodulatory activities	434:478	antioxidative and immunomodulatory activities	434:478	Polysaccharides of Ganoderma lucidum strain S3 (GLPS3) possess antioxidative and immunomodulatory activities.
26826268	9	15	theme	interferon-gamma	1462:1477	arg1	contents					1487:1494	interleukin-1beta (IL-1β) and interferon-gamma (INF-γ) contents	1432:1494	interleukin-1beta (IL-1β) and interferon-gamma (INF-γ) contents	1432:1494	Moreover, interleukin-1beta (IL-1β) and interferon-gamma (INF-γ) contents were reduced most by GLPS3-Ⅱ.
26826268	5	16	theme	GLPS3	648:652	arg1	%					752:752	90.4%	748:752	90.4%	748:752	The total sugar content of GLPS3 from fermentation broth (GLPS3-Ⅰ), cultured mycelia (GLPS3-Ⅱ) and fruiting body (GLPS3-Ⅲ) was 90.4%, 92.2% and 91.8% respectively.
26826268	5	16	theme	GLPS3	648:652	arg1	content					637:643	The total sugar content	621:643	The total sugar content of GLPS3 from fermentation broth (GLPS3-Ⅰ), cultured mycelia (GLPS3-Ⅱ) and fruiting body (GLPS3-Ⅲ)	621:742	The total sugar content of GLPS3 from fermentation broth (GLPS3-Ⅰ), cultured mycelia (GLPS3-Ⅱ) and fruiting body (GLPS3-Ⅲ) was 90.4%, 92.2% and 91.8% respectively.
26826268	5	17	from	body	729:732	arg1	%					752:752	90.4%	748:752	90.4%	748:752	The total sugar content of GLPS3 from fermentation broth (GLPS3-Ⅰ), cultured mycelia (GLPS3-Ⅱ) and fruiting body (GLPS3-Ⅲ) was 90.4%, 92.2% and 91.8% respectively.
26826268	5	17	from	body	729:732	arg1	GLPS3					648:652	GLPS3	648:652	GLPS3 from fermentation broth (GLPS3-Ⅰ), cultured mycelia (GLPS3-Ⅱ) and fruiting body (GLPS3-Ⅲ)	648:742	The total sugar content of GLPS3 from fermentation broth (GLPS3-Ⅰ), cultured mycelia (GLPS3-Ⅱ) and fruiting body (GLPS3-Ⅲ) was 90.4%, 92.2% and 91.8% respectively.
26826268	5	17	from	body	729:732	arg1	content					637:643	The total sugar content	621:643	The total sugar content of GLPS3 from fermentation broth (GLPS3-Ⅰ), cultured mycelia (GLPS3-Ⅱ) and fruiting body (GLPS3-Ⅲ)	621:742	The total sugar content of GLPS3 from fermentation broth (GLPS3-Ⅰ), cultured mycelia (GLPS3-Ⅱ) and fruiting body (GLPS3-Ⅲ) was 90.4%, 92.2% and 91.8% respectively.
26826268	3	18	theme	strain	408:413	arg1	GLPS3					419:423	GLPS3	419:423	GLPS3	419:423	Polysaccharides of Ganoderma lucidum strain S3 (GLPS3) possess antioxidative and immunomodulatory activities.
26826268	3	18	theme	strain	408:413	arg1	S3					415:416	Ganoderma lucidum strain S3	390:416	Ganoderma lucidum strain S3 (GLPS3)	390:424	Polysaccharides of Ganoderma lucidum strain S3 (GLPS3) possess antioxidative and immunomodulatory activities.
26826268	4	19	theme	chemical	512:519	arg1	structures					521:530	chemical structures	512:530	chemical structures of GLPS3	512:539	This study was to characterize chemical structures of GLPS3 and determine their effects on diethyldithiocarbamate (DDC)-induced CP in mice.
26826268	2	20	theme	weight	326:331	arg1	loss					333:336	weight loss	326:336	weight loss	326:336	Patients with chronic pancreatitis often present with abdominal pain, diarrhoea, jaundice, weight loss and the development of diabetes.
26826268	1	21	theme	irreversible	186:197	arg1	change					211:216	irreversible morphologic change	186:216	irreversible morphologic change	186:216	Chronic pancreatitis (CP) is a progressive inflammation of pancreas characterized by irreversible morphologic change and dysfunction.
26826268	3	22	theme	S3	415:416	arg1	Polysaccharides					371:385	Polysaccharides	371:385	Polysaccharides of Ganoderma lucidum strain S3 (GLPS3)	371:424	Polysaccharides of Ganoderma lucidum strain S3 (GLPS3) possess antioxidative and immunomodulatory activities.
26826268	0	23	theme	Ganoderma	15:23	arg1	lucidum					25:31	Ganoderma lucidum	15:31	Ganoderma lucidum polysaccharides	15:47	Three kinds of Ganoderma lucidum polysaccharides attenuate DDC-induced chronic pancreatitis in mice.
26826268	7	24	theme	superoxide	1148:1157	arg1	SOD					1170:1172	SOD	1170:1172	SOD	1170:1172	The superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in serum were increased while the malondialdehyde (MDA) levels were reversely decreased by GLPS3 treatment.
26826268	7	24	theme	superoxide	1148:1157	arg1	dismutase					1159:1167	The superoxide dismutase	1144:1167	The superoxide dismutase (SOD)	1144:1173	The superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in serum were increased while the malondialdehyde (MDA) levels were reversely decreased by GLPS3 treatment.
26826268	1	25	theme	Chronic	101:107	arg1	inflammation					144:155	a progressive inflammation	130:155	a progressive inflammation of pancreas characterized by irreversible morphologic change and dysfunction	130:232	Chronic pancreatitis (CP) is a progressive inflammation of pancreas characterized by irreversible morphologic change and dysfunction.
26826268	1	25	theme	Chronic	101:107	arg1	CP					123:124	CP	123:124	CP	123:124	Chronic pancreatitis (CP) is a progressive inflammation of pancreas characterized by irreversible morphologic change and dysfunction.
26826268	1	25	theme	Chronic	101:107	arg1	pancreatitis					109:120	Chronic pancreatitis	101:120	Chronic pancreatitis (CP)	101:125	Chronic pancreatitis (CP) is a progressive inflammation of pancreas characterized by irreversible morphologic change and dysfunction.
26826268	1	26	theme	morphologic	199:209	arg1	change					211:216	irreversible morphologic change	186:216	irreversible morphologic change	186:216	Chronic pancreatitis (CP) is a progressive inflammation of pancreas characterized by irreversible morphologic change and dysfunction.
26826268	3	27	theme	Ganoderma	390:398	arg1	lucidum					400:406	Ganoderma lucidum	390:406	Ganoderma lucidum strain S3 (GLPS3)	390:424	Polysaccharides of Ganoderma lucidum strain S3 (GLPS3) possess antioxidative and immunomodulatory activities.
26826268	2	28	with	Patients	235:242	arg1	pancreatitis					257:268	chronic pancreatitis	249:268	chronic pancreatitis	249:268	Patients with chronic pancreatitis often present with abdominal pain, diarrhoea, jaundice, weight loss and the development of diabetes.
26826268	4	29	from	effects	561:567	arg1	mice					615:618	mice	615:618	mice	615:618	This study was to characterize chemical structures of GLPS3 and determine their effects on diethyldithiocarbamate (DDC)-induced CP in mice.
26826268	4	29	from	effects	561:567	arg1	CP					609:610	diethyldithiocarbamate (DDC)-induced CP	572:610	diethyldithiocarbamate (DDC)-induced CP	572:610	This study was to characterize chemical structures of GLPS3 and determine their effects on diethyldithiocarbamate (DDC)-induced CP in mice.
26826268	3	30	theme	lucidum	400:406	arg1	GLPS3					419:423	GLPS3	419:423	GLPS3	419:423	Polysaccharides of Ganoderma lucidum strain S3 (GLPS3) possess antioxidative and immunomodulatory activities.
26826268	3	30	theme	lucidum	400:406	arg1	S3					415:416	Ganoderma lucidum strain S3	390:416	Ganoderma lucidum strain S3 (GLPS3)	390:424	Polysaccharides of Ganoderma lucidum strain S3 (GLPS3) possess antioxidative and immunomodulatory activities.
26826268	4	31	theme	-induced	600:607	arg1	CP					609:610	diethyldithiocarbamate (DDC)-induced CP	572:610	diethyldithiocarbamate (DDC)-induced CP	572:610	This study was to characterize chemical structures of GLPS3 and determine their effects on diethyldithiocarbamate (DDC)-induced CP in mice.
26826268	1	32	theme	pancreas	160:167	arg1	inflammation					144:155	a progressive inflammation	130:155	a progressive inflammation of pancreas characterized by irreversible morphologic change and dysfunction	130:232	Chronic pancreatitis (CP) is a progressive inflammation of pancreas characterized by irreversible morphologic change and dysfunction.
26826268	1	32	theme	pancreas	160:167	arg1	pancreatitis					109:120	Chronic pancreatitis	101:120	Chronic pancreatitis (CP)	101:125	Chronic pancreatitis (CP) is a progressive inflammation of pancreas characterized by irreversible morphologic change and dysfunction.
26826268	0	33	theme	polysaccharides	33:47	arg1	kinds					6:10	Three kinds	0:10	Three kinds of Ganoderma lucidum polysaccharides	0:47	Three kinds of Ganoderma lucidum polysaccharides attenuate DDC-induced chronic pancreatitis in mice.
26826268	7	34	from	dismutase	1159:1167	arg1	serum					1214:1218	serum	1214:1218	serum	1214:1218	The superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in serum were increased while the malondialdehyde (MDA) levels were reversely decreased by GLPS3 treatment.
26826268	7	35	from	peroxidase	1191:1200	arg1	serum					1214:1218	serum	1214:1218	serum	1214:1218	The superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in serum were increased while the malondialdehyde (MDA) levels were reversely decreased by GLPS3 treatment.
26826268	0	36	theme	lucidum	25:31	arg1	polysaccharides					33:47	Ganoderma lucidum polysaccharides	15:47	Ganoderma lucidum polysaccharides	15:47	Three kinds of Ganoderma lucidum polysaccharides attenuate DDC-induced chronic pancreatitis in mice.
26826268	5	37	theme	fruiting	720:727	arg1	GLPS3-Ⅲ					735:741	GLPS3-Ⅲ	735:741	GLPS3-Ⅲ	735:741	The total sugar content of GLPS3 from fermentation broth (GLPS3-Ⅰ), cultured mycelia (GLPS3-Ⅱ) and fruiting body (GLPS3-Ⅲ) was 90.4%, 92.2% and 91.8% respectively.
26826268	5	37	theme	fruiting	720:727	arg1	body					729:732	fruiting body	720:732	fruiting body (GLPS3-Ⅲ)	720:742	The total sugar content of GLPS3 from fermentation broth (GLPS3-Ⅰ), cultured mycelia (GLPS3-Ⅱ) and fruiting body (GLPS3-Ⅲ) was 90.4%, 92.2% and 91.8% respectively.
26826268	0	38	from	pancreatitis	79:90	arg1	mice					95:98	mice	95:98	mice	95:98	Three kinds of Ganoderma lucidum polysaccharides attenuate DDC-induced chronic pancreatitis in mice.
26826268	2	39	theme	chronic	249:255	arg1	pancreatitis					257:268	chronic pancreatitis	249:268	chronic pancreatitis	249:268	Patients with chronic pancreatitis often present with abdominal pain, diarrhoea, jaundice, weight loss and the development of diabetes.
26826268	0	40	theme	DDC-induced	59:69	arg1	pancreatitis					79:90	DDC-induced chronic pancreatitis	59:90	DDC-induced chronic pancreatitis in mice	59:98	Three kinds of Ganoderma lucidum polysaccharides attenuate DDC-induced chronic pancreatitis in mice.
26826268	8	41	theme	Serum	1319:1323	arg1	AMS					1334:1336	AMS	1334:1336	AMS	1334:1336	Serum amylase (AMS) and lactic dehydrogenase (LDH) changes indicated the therapeutic effects of GLPS3.
26826268	8	41	theme	Serum	1319:1323	arg1	amylase					1325:1331	Serum amylase	1319:1331	Serum amylase (AMS)	1319:1337	Serum amylase (AMS) and lactic dehydrogenase (LDH) changes indicated the therapeutic effects of GLPS3.
26826268	6	42	theme	cultured	1076:1083	arg1	mycelia					1085:1091	GLPS3-Ⅱfrom cultured mycelia	1064:1091	GLPS3-Ⅱfrom cultured mycelia	1064:1091	The antioxidative activity of GLPS3-Ⅱfrom cultured mycelia in vitro is higher than other two polysaccharides.
26826268	9	43	theme	interleukin-1beta	1432:1448	arg1	contents					1487:1494	interleukin-1beta (IL-1β) and interferon-gamma (INF-γ) contents	1432:1494	interleukin-1beta (IL-1β) and interferon-gamma (INF-γ) contents	1432:1494	Moreover, interleukin-1beta (IL-1β) and interferon-gamma (INF-γ) contents were reduced most by GLPS3-Ⅱ.
26826268	8	44	theme	GLPS3	1415:1419	arg1	effects					1404:1410	the therapeutic effects	1388:1410	the therapeutic effects of GLPS3	1388:1419	Serum amylase (AMS) and lactic dehydrogenase (LDH) changes indicated the therapeutic effects of GLPS3.
26826268	5	45	from	broth	672:676	arg1	%					752:752	90.4%	748:752	90.4%	748:752	The total sugar content of GLPS3 from fermentation broth (GLPS3-Ⅰ), cultured mycelia (GLPS3-Ⅱ) and fruiting body (GLPS3-Ⅲ) was 90.4%, 92.2% and 91.8% respectively.
26826268	5	45	from	broth	672:676	arg1	GLPS3					648:652	GLPS3	648:652	GLPS3 from fermentation broth (GLPS3-Ⅰ), cultured mycelia (GLPS3-Ⅱ) and fruiting body (GLPS3-Ⅲ)	648:742	The total sugar content of GLPS3 from fermentation broth (GLPS3-Ⅰ), cultured mycelia (GLPS3-Ⅱ) and fruiting body (GLPS3-Ⅲ) was 90.4%, 92.2% and 91.8% respectively.
26826268	5	45	from	broth	672:676	arg1	content					637:643	The total sugar content	621:643	The total sugar content of GLPS3 from fermentation broth (GLPS3-Ⅰ), cultured mycelia (GLPS3-Ⅱ) and fruiting body (GLPS3-Ⅲ)	621:742	The total sugar content of GLPS3 from fermentation broth (GLPS3-Ⅰ), cultured mycelia (GLPS3-Ⅱ) and fruiting body (GLPS3-Ⅲ) was 90.4%, 92.2% and 91.8% respectively.
26826268	5	46	theme	cultured	689:696	arg1	mycelia					698:704	cultured mycelia	689:704	cultured mycelia (GLPS3-Ⅱ)	689:714	The total sugar content of GLPS3 from fermentation broth (GLPS3-Ⅰ), cultured mycelia (GLPS3-Ⅱ) and fruiting body (GLPS3-Ⅲ) was 90.4%, 92.2% and 91.8% respectively.
26826268	5	46	theme	cultured	689:696	arg1	GLPS3-Ⅱ					707:713	GLPS3-Ⅱ	707:713	GLPS3-Ⅱ	707:713	The total sugar content of GLPS3 from fermentation broth (GLPS3-Ⅰ), cultured mycelia (GLPS3-Ⅱ) and fruiting body (GLPS3-Ⅲ) was 90.4%, 92.2% and 91.8% respectively.
26826268	6	47	theme	GLPS3-Ⅱfrom	1064:1074	arg1	mycelia					1085:1091	GLPS3-Ⅱfrom cultured mycelia	1064:1091	GLPS3-Ⅱfrom cultured mycelia	1064:1091	The antioxidative activity of GLPS3-Ⅱfrom cultured mycelia in vitro is higher than other two polysaccharides.
26826268	5	48	theme	fermentation	659:670	arg1	GLPS3-Ⅰ					679:685	GLPS3-Ⅰ	679:685	GLPS3-Ⅰ	679:685	The total sugar content of GLPS3 from fermentation broth (GLPS3-Ⅰ), cultured mycelia (GLPS3-Ⅱ) and fruiting body (GLPS3-Ⅲ) was 90.4%, 92.2% and 91.8% respectively.
26826268	5	48	theme	fermentation	659:670	arg1	broth					672:676	fermentation broth	659:676	fermentation broth (GLPS3-Ⅰ)	659:686	The total sugar content of GLPS3 from fermentation broth (GLPS3-Ⅰ), cultured mycelia (GLPS3-Ⅱ) and fruiting body (GLPS3-Ⅲ) was 90.4%, 92.2% and 91.8% respectively.
26826268	2	49	theme	abdominal	289:297	arg1	pain					299:302	abdominal pain	289:302	abdominal pain	289:302	Patients with chronic pancreatitis often present with abdominal pain, diarrhoea, jaundice, weight loss and the development of diabetes.
26826268	5	50	theme	total	625:629	arg1	%					752:752	90.4%	748:752	90.4%	748:752	The total sugar content of GLPS3 from fermentation broth (GLPS3-Ⅰ), cultured mycelia (GLPS3-Ⅱ) and fruiting body (GLPS3-Ⅲ) was 90.4%, 92.2% and 91.8% respectively.
26826268	5	50	theme	total	625:629	arg1	content					637:643	The total sugar content	621:643	The total sugar content of GLPS3 from fermentation broth (GLPS3-Ⅰ), cultured mycelia (GLPS3-Ⅱ) and fruiting body (GLPS3-Ⅲ)	621:742	The total sugar content of GLPS3 from fermentation broth (GLPS3-Ⅰ), cultured mycelia (GLPS3-Ⅱ) and fruiting body (GLPS3-Ⅲ) was 90.4%, 92.2% and 91.8% respectively.
26826268	10	51	theme	cultured	1581:1588	arg1	mycelia					1590:1596	GLPS3 especially GLPS3-Ⅱfrom cultured mycelia	1552:1596	GLPS3 especially GLPS3-Ⅱfrom cultured mycelia	1552:1596	The results revealed that GLPS3 especially GLPS3-Ⅱfrom cultured mycelia were effective for CP therapy and bioactivity difference might be attributed to monosaccharide composition.
26826268	5	52	from	mycelia	698:704	arg1	%					752:752	90.4%	748:752	90.4%	748:752	The total sugar content of GLPS3 from fermentation broth (GLPS3-Ⅰ), cultured mycelia (GLPS3-Ⅱ) and fruiting body (GLPS3-Ⅲ) was 90.4%, 92.2% and 91.8% respectively.
26826268	5	52	from	mycelia	698:704	arg1	GLPS3					648:652	GLPS3	648:652	GLPS3 from fermentation broth (GLPS3-Ⅰ), cultured mycelia (GLPS3-Ⅱ) and fruiting body (GLPS3-Ⅲ)	648:742	The total sugar content of GLPS3 from fermentation broth (GLPS3-Ⅰ), cultured mycelia (GLPS3-Ⅱ) and fruiting body (GLPS3-Ⅲ) was 90.4%, 92.2% and 91.8% respectively.
26826268	5	52	from	mycelia	698:704	arg1	content					637:643	The total sugar content	621:643	The total sugar content of GLPS3 from fermentation broth (GLPS3-Ⅰ), cultured mycelia (GLPS3-Ⅱ) and fruiting body (GLPS3-Ⅲ)	621:742	The total sugar content of GLPS3 from fermentation broth (GLPS3-Ⅰ), cultured mycelia (GLPS3-Ⅱ) and fruiting body (GLPS3-Ⅲ) was 90.4%, 92.2% and 91.8% respectively.
26826268	7	53	theme	malondialdehyde	1245:1259	arg1	levels					1267:1272	the malondialdehyde (MDA) levels	1241:1272	the malondialdehyde (MDA) levels	1241:1272	The superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in serum were increased while the malondialdehyde (MDA) levels were reversely decreased by GLPS3 treatment.
26826268	10	54	theme	monosaccharide	1678:1691	arg1	composition					1693:1703	monosaccharide composition	1678:1703	monosaccharide composition	1678:1703	The results revealed that GLPS3 especially GLPS3-Ⅱfrom cultured mycelia were effective for CP therapy and bioactivity difference might be attributed to monosaccharide composition.
26826268	6	55	theme	antioxidative	1038:1050	arg1	activity					1052:1059	The antioxidative activity	1034:1059	The antioxidative activity of GLPS3-Ⅱfrom cultured mycelia in vitro	1034:1100	The antioxidative activity of GLPS3-Ⅱfrom cultured mycelia in vitro is higher than other two polysaccharides.
26826268	6	55	theme	antioxidative	1038:1050	arg1	higher					1105:1110	higher	1105:1110	higher	1105:1110	The antioxidative activity of GLPS3-Ⅱfrom cultured mycelia in vitro is higher than other two polysaccharides.
26826268	10	56	theme	GLPS3	1552:1556	arg1	mycelia					1590:1596	GLPS3 especially GLPS3-Ⅱfrom cultured mycelia	1552:1596	GLPS3 especially GLPS3-Ⅱfrom cultured mycelia	1552:1596	The results revealed that GLPS3 especially GLPS3-Ⅱfrom cultured mycelia were effective for CP therapy and bioactivity difference might be attributed to monosaccharide composition.
26826268	1	57	theme	progressive	132:142	arg1	inflammation					144:155	a progressive inflammation	130:155	a progressive inflammation of pancreas characterized by irreversible morphologic change and dysfunction	130:232	Chronic pancreatitis (CP) is a progressive inflammation of pancreas characterized by irreversible morphologic change and dysfunction.
26826268	1	57	theme	progressive	132:142	arg1	pancreatitis					109:120	Chronic pancreatitis	101:120	Chronic pancreatitis (CP)	101:125	Chronic pancreatitis (CP) is a progressive inflammation of pancreas characterized by irreversible morphologic change and dysfunction.
26826268	0	58	theme	chronic	71:77	arg1	pancreatitis					79:90	DDC-induced chronic pancreatitis	59:90	DDC-induced chronic pancreatitis in mice	59:98	Three kinds of Ganoderma lucidum polysaccharides attenuate DDC-induced chronic pancreatitis in mice.
26826268	4	59	theme	GLPS3	535:539	arg1	structures					521:530	chemical structures	512:530	chemical structures of GLPS3	512:539	This study was to characterize chemical structures of GLPS3 and determine their effects on diethyldithiocarbamate (DDC)-induced CP in mice.
26826268	5	60	from	content	637:643	arg1	mycelia					698:704	cultured mycelia	689:704	cultured mycelia (GLPS3-Ⅱ)	689:714	The total sugar content of GLPS3 from fermentation broth (GLPS3-Ⅰ), cultured mycelia (GLPS3-Ⅱ) and fruiting body (GLPS3-Ⅲ) was 90.4%, 92.2% and 91.8% respectively.
26826268	5	60	from	content	637:643	arg1	body					729:732	fruiting body	720:732	fruiting body (GLPS3-Ⅲ)	720:742	The total sugar content of GLPS3 from fermentation broth (GLPS3-Ⅰ), cultured mycelia (GLPS3-Ⅱ) and fruiting body (GLPS3-Ⅲ) was 90.4%, 92.2% and 91.8% respectively.
26826268	5	60	from	content	637:643	arg1	broth					672:676	fermentation broth	659:676	fermentation broth (GLPS3-Ⅰ)	659:686	The total sugar content of GLPS3 from fermentation broth (GLPS3-Ⅰ), cultured mycelia (GLPS3-Ⅱ) and fruiting body (GLPS3-Ⅲ) was 90.4%, 92.2% and 91.8% respectively.
26826268	5	60	from	content	637:643	arg1	GLPS3-Ⅲ					735:741	GLPS3-Ⅲ	735:741	GLPS3-Ⅲ	735:741	The total sugar content of GLPS3 from fermentation broth (GLPS3-Ⅰ), cultured mycelia (GLPS3-Ⅱ) and fruiting body (GLPS3-Ⅲ) was 90.4%, 92.2% and 91.8% respectively.
26826268	5	60	from	content	637:643	arg1	GLPS3-Ⅰ					679:685	GLPS3-Ⅰ	679:685	GLPS3-Ⅰ	679:685	The total sugar content of GLPS3 from fermentation broth (GLPS3-Ⅰ), cultured mycelia (GLPS3-Ⅱ) and fruiting body (GLPS3-Ⅲ) was 90.4%, 92.2% and 91.8% respectively.
26826268	5	60	from	content	637:643	arg1	GLPS3-Ⅱ					707:713	GLPS3-Ⅱ	707:713	GLPS3-Ⅱ	707:713	The total sugar content of GLPS3 from fermentation broth (GLPS3-Ⅰ), cultured mycelia (GLPS3-Ⅱ) and fruiting body (GLPS3-Ⅲ) was 90.4%, 92.2% and 91.8% respectively.
26826268	10	61	dep	cultured	1581:1588	arg1	GLPS3-Ⅱfrom					1569:1579	GLPS3-Ⅱfrom	1569:1579	GLPS3-Ⅱfrom	1569:1579	The results revealed that GLPS3 especially GLPS3-Ⅱfrom cultured mycelia were effective for CP therapy and bioactivity difference might be attributed to monosaccharide composition.
26014143	2	0	from	effect	176:181	arg1	flow					281:284	steady shear flow	268:284	steady shear flow	268:284	The effect of pH (3.5-10.0) and ionic strength (0.02-1.0 M NaCl) on the intrinsic viscosity and steady shear flow were evaluated using a central composite rotatable design of experiments and surface response methodology.
26014143	2	0	from	effect	176:181	arg1	viscosity					254:262	the intrinsic viscosity	240:262	the intrinsic viscosity	240:262	The effect of pH (3.5-10.0) and ionic strength (0.02-1.0 M NaCl) on the intrinsic viscosity and steady shear flow were evaluated using a central composite rotatable design of experiments and surface response methodology.
26014143	6	1	theme	Flow	667:670	arg1	curves					672:677	Flow curves	667:677	Flow curves	667:677	Flow curves were successfully described by the Cross model.
26014143	2	2	theme	central	309:315	arg1	design					337:342	a central composite rotatable design	307:342	a central composite rotatable design of experiments	307:357	The effect of pH (3.5-10.0) and ionic strength (0.02-1.0 M NaCl) on the intrinsic viscosity and steady shear flow were evaluated using a central composite rotatable design of experiments and surface response methodology.
26014143	9	3	theme	apparent	1106:1113	arg1	viscosity					1115:1123	the apparent viscosity	1102:1123	the apparent viscosity	1102:1123	Given the results obtained, FucoPol is proposed as thickening agent for applications in which stability of the apparent viscosity under pH and ionic strength variations is required.
26014143	9	4	theme	ionic	1138:1142	arg1	strength					1144:1151	ionic strength	1138:1151	ionic strength	1138:1151	Given the results obtained, FucoPol is proposed as thickening agent for applications in which stability of the apparent viscosity under pH and ionic strength variations is required.
26014143	4	5	with	solutions	576:584	arg1	behavior					606:613	shear-thinning behavior	591:613	shear-thinning behavior	591:613	FucoPol produced viscous solutions with shear-thinning behavior at different polymer concentrations (0.2-1.2 wt.
26014143	8	6	theme	time	897:900	arg1	dependence					858:867	the dependence	854:867	the dependence of the estimated relaxation time with polymer concentration	854:927	%, and the dependence of the estimated relaxation time with polymer concentration suggests a large degree of interaction between FucoPol molecules.
26014143	3	7	theme	wide	478:481	arg1	range					483:487	a wide range	476:487	a wide range of pH (3.5-8.0) and ionic strength (0.05-0.50 M NaCl) values	476:548	FucoPol's intrinsic and apparent viscosities presented a quite low variation under a wide range of pH (3.5-8.0) and ionic strength (0.05-0.50 M NaCl) values.
26014143	7	8	theme	polymer	805:811	arg1	concentrations					813:826	polymer concentrations	805:826	polymer concentrations from 0.2 to 1.2 wt	805:845	The viscosity of the first Newtonian plateau varied from 0.01 to 2.47 Pas for polymer concentrations from 0.2 to 1.2 wt.
26014143	3	9	dep	intrinsic	403:411	arg1	's					400:401	's	400:401	's	400:401	FucoPol's intrinsic and apparent viscosities presented a quite low variation under a wide range of pH (3.5-8.0) and ionic strength (0.05-0.50 M NaCl) values.
26014143	2	10	theme	intrinsic	244:252	arg1	viscosity					254:262	the intrinsic viscosity	240:262	the intrinsic viscosity	240:262	The effect of pH (3.5-10.0) and ionic strength (0.02-1.0 M NaCl) on the intrinsic viscosity and steady shear flow were evaluated using a central composite rotatable design of experiments and surface response methodology.
26014143	3	11	theme	strength	515:522	arg1	values					543:548	pH (3.5-8.0) and ionic strength (0.05-0.50 M NaCl) values	492:548	pH (3.5-8.0) and ionic strength (0.05-0.50 M NaCl) values	492:548	FucoPol's intrinsic and apparent viscosities presented a quite low variation under a wide range of pH (3.5-8.0) and ionic strength (0.05-0.50 M NaCl) values.
26014143	8	12	theme	interaction	956:966	arg1	degree					946:951	a large degree	938:951	a large degree of interaction between FucoPol molecules	938:992	%, and the dependence of the estimated relaxation time with polymer concentration suggests a large degree of interaction between FucoPol molecules.
26014143	8	13	theme	polymer	907:913	arg1	concentration					915:927	polymer concentration	907:927	polymer concentration	907:927	%, and the dependence of the estimated relaxation time with polymer concentration suggests a large degree of interaction between FucoPol molecules.
26014143	3	14	theme	low	456:458	arg1	variation					460:468	a quite low variation	448:468	a quite low variation	448:468	FucoPol's intrinsic and apparent viscosities presented a quite low variation under a wide range of pH (3.5-8.0) and ionic strength (0.05-0.50 M NaCl) values.
26014143	4	15	theme	shear-thinning	591:604	arg1	behavior					606:613	shear-thinning behavior	591:613	shear-thinning behavior	591:613	FucoPol produced viscous solutions with shear-thinning behavior at different polymer concentrations (0.2-1.2 wt.
26014143	3	16	theme	pH	492:493	arg1	values					543:548	pH (3.5-8.0) and ionic strength (0.05-0.50 M NaCl) values	492:548	pH (3.5-8.0) and ionic strength (0.05-0.50 M NaCl) values	492:548	FucoPol's intrinsic and apparent viscosities presented a quite low variation under a wide range of pH (3.5-8.0) and ionic strength (0.05-0.50 M NaCl) values.
26014143	8	17	with	dependence	858:867	arg1	concentration					915:927	polymer concentration	907:927	polymer concentration	907:927	%, and the dependence of the estimated relaxation time with polymer concentration suggests a large degree of interaction between FucoPol molecules.
26014143	8	18	theme	FucoPol	976:982	arg1	molecules					984:992	FucoPol molecules	976:992	FucoPol molecules	976:992	%, and the dependence of the estimated relaxation time with polymer concentration suggests a large degree of interaction between FucoPol molecules.
26014143	4	19	dep	concentrations	636:649	arg1	wt					660:661	0.2-1.2 wt	652:661	0.2-1.2 wt	652:661	FucoPol produced viscous solutions with shear-thinning behavior at different polymer concentrations (0.2-1.2 wt.
26014143	2	20	theme	experiments	347:357	arg1	design					337:342	a central composite rotatable design	307:342	a central composite rotatable design of experiments	307:357	The effect of pH (3.5-10.0) and ionic strength (0.02-1.0 M NaCl) on the intrinsic viscosity and steady shear flow were evaluated using a central composite rotatable design of experiments and surface response methodology.
26014143	2	20	theme	experiments	347:357	arg1	methodology					380:390	surface response methodology	363:390	surface response methodology	363:390	The effect of pH (3.5-10.0) and ionic strength (0.02-1.0 M NaCl) on the intrinsic viscosity and steady shear flow were evaluated using a central composite rotatable design of experiments and surface response methodology.
26014143	8	21	theme	large	940:944	arg1	degree					946:951	a large degree	938:951	a large degree of interaction between FucoPol molecules	938:992	%, and the dependence of the estimated relaxation time with polymer concentration suggests a large degree of interaction between FucoPol molecules.
26014143	2	22	theme	shear	275:279	arg1	flow					281:284	steady shear flow	268:284	steady shear flow	268:284	The effect of pH (3.5-10.0) and ionic strength (0.02-1.0 M NaCl) on the intrinsic viscosity and steady shear flow were evaluated using a central composite rotatable design of experiments and surface response methodology.
26014143	4	23	theme	polymer	628:634	arg1	concentrations					636:649	different polymer concentrations	618:649	different polymer concentrations (0.2-1.2 wt	618:661	FucoPol produced viscous solutions with shear-thinning behavior at different polymer concentrations (0.2-1.2 wt.
26014143	0	24	theme	Rheological	0:10	arg1	studies					12:18	Rheological studies	0:18	Rheological studies of the fucose-rich exopolysaccharide FucoPol	0:63	Rheological studies of the fucose-rich exopolysaccharide FucoPol.
26014143	7	25	theme	first	748:752	arg1	plateau					764:770	the first Newtonian plateau	744:770	the first Newtonian plateau	744:770	The viscosity of the first Newtonian plateau varied from 0.01 to 2.47 Pas for polymer concentrations from 0.2 to 1.2 wt.
26014143	8	26	theme	relaxation	886:895	arg1	time					897:900	the estimated relaxation time	872:900	the estimated relaxation time	872:900	%, and the dependence of the estimated relaxation time with polymer concentration suggests a large degree of interaction between FucoPol molecules.
26014143	2	27	theme	steady	268:273	arg1	flow					281:284	steady shear flow	268:284	steady shear flow	268:284	The effect of pH (3.5-10.0) and ionic strength (0.02-1.0 M NaCl) on the intrinsic viscosity and steady shear flow were evaluated using a central composite rotatable design of experiments and surface response methodology.
26014143	4	28	theme	different	618:626	arg1	concentrations					636:649	different polymer concentrations	618:649	different polymer concentrations (0.2-1.2 wt	618:661	FucoPol produced viscous solutions with shear-thinning behavior at different polymer concentrations (0.2-1.2 wt.
26014143	3	29	theme	values	543:548	arg1	range					483:487	a wide range	476:487	a wide range of pH (3.5-8.0) and ionic strength (0.05-0.50 M NaCl) values	476:548	FucoPol's intrinsic and apparent viscosities presented a quite low variation under a wide range of pH (3.5-8.0) and ionic strength (0.05-0.50 M NaCl) values.
26014143	7	30	from	wt	844:845	arg1	concentrations					813:826	polymer concentrations	805:826	polymer concentrations from 0.2 to 1.2 wt	805:845	The viscosity of the first Newtonian plateau varied from 0.01 to 2.47 Pas for polymer concentrations from 0.2 to 1.2 wt.
26014143	1	31	theme	solution	84:91	arg1	properties					93:102	the solution properties	80:102	the solution properties of the bacterial fucose-rich polysaccharide, FucoPol,	80:156	In this work, the solution properties of the bacterial fucose-rich polysaccharide, FucoPol, were studied.
26014143	3	32	theme	ionic	509:513	arg1	NaCl					537:540	0.05-0.50 M NaCl	525:540	0.05-0.50 M NaCl	525:540	FucoPol's intrinsic and apparent viscosities presented a quite low variation under a wide range of pH (3.5-8.0) and ionic strength (0.05-0.50 M NaCl) values.
26014143	3	32	theme	ionic	509:513	arg1	strength					515:522	ionic strength	509:522	ionic strength (0.05-0.50 M NaCl)	509:541	FucoPol's intrinsic and apparent viscosities presented a quite low variation under a wide range of pH (3.5-8.0) and ionic strength (0.05-0.50 M NaCl) values.
26014143	7	33	theme	plateau	764:770	arg1	viscosity					731:739	The viscosity	727:739	The viscosity of the first Newtonian plateau	727:770	The viscosity of the first Newtonian plateau varied from 0.01 to 2.47 Pas for polymer concentrations from 0.2 to 1.2 wt.
26014143	4	34	theme	viscous	568:574	arg1	solutions					576:584	viscous solutions	568:584	viscous solutions with shear-thinning behavior	568:613	FucoPol produced viscous solutions with shear-thinning behavior at different polymer concentrations (0.2-1.2 wt.
26014143	0	35	theme	exopolysaccharide	39:55	arg1	FucoPol					57:63	the fucose-rich exopolysaccharide FucoPol	23:63	the fucose-rich exopolysaccharide FucoPol	23:63	Rheological studies of the fucose-rich exopolysaccharide FucoPol.
26014143	3	36	theme	apparent	417:424	arg1	viscosities					426:436	FucoPol's intrinsic and apparent viscosities	393:436	viscosities	426:436	FucoPol's intrinsic and apparent viscosities presented a quite low variation under a wide range of pH (3.5-8.0) and ionic strength (0.05-0.50 M NaCl) values.
26014143	2	37	theme	strength	210:217	arg1	effect					176:181	The effect	172:181	The effect of pH (3.5-10.0) and ionic strength (0.02-1.0 M NaCl) on the intrinsic viscosity and steady shear flow	172:284	The effect of pH (3.5-10.0) and ionic strength (0.02-1.0 M NaCl) on the intrinsic viscosity and steady shear flow were evaluated using a central composite rotatable design of experiments and surface response methodology.
26014143	2	38	theme	response	371:378	arg1	methodology					380:390	surface response methodology	363:390	surface response methodology	363:390	The effect of pH (3.5-10.0) and ionic strength (0.02-1.0 M NaCl) on the intrinsic viscosity and steady shear flow were evaluated using a central composite rotatable design of experiments and surface response methodology.
26014143	0	39	theme	fucose-rich	27:37	arg1	FucoPol					57:63	the fucose-rich exopolysaccharide FucoPol	23:63	the fucose-rich exopolysaccharide FucoPol	23:63	Rheological studies of the fucose-rich exopolysaccharide FucoPol.
26014143	9	40	theme	thickening	1046:1055	arg1	agent					1057:1061	thickening agent	1046:1061	thickening agent for applications in which stability of the apparent viscosity under pH and ionic strength variations is required	1046:1174	Given the results obtained, FucoPol is proposed as thickening agent for applications in which stability of the apparent viscosity under pH and ionic strength variations is required.
26014143	9	40	theme	thickening	1046:1055	arg1	FucoPol					1023:1029	FucoPol	1023:1029	FucoPol	1023:1029	Given the results obtained, FucoPol is proposed as thickening agent for applications in which stability of the apparent viscosity under pH and ionic strength variations is required.
26014143	2	41	theme	ionic	204:208	arg1	strength					210:217	ionic strength	204:217	ionic strength (0.02-1.0 M NaCl)	204:235	The effect of pH (3.5-10.0) and ionic strength (0.02-1.0 M NaCl) on the intrinsic viscosity and steady shear flow were evaluated using a central composite rotatable design of experiments and surface response methodology.
26014143	2	41	theme	ionic	204:208	arg1	NaCl					231:234	0.02-1.0 M NaCl	220:234	0.02-1.0 M NaCl	220:234	The effect of pH (3.5-10.0) and ionic strength (0.02-1.0 M NaCl) on the intrinsic viscosity and steady shear flow were evaluated using a central composite rotatable design of experiments and surface response methodology.
26014143	6	42	theme	Cross	714:718	arg1	model					720:724	the Cross model	710:724	the Cross model	710:724	Flow curves were successfully described by the Cross model.
26014143	2	43	theme	surface	363:369	arg1	methodology					380:390	surface response methodology	363:390	surface response methodology	363:390	The effect of pH (3.5-10.0) and ionic strength (0.02-1.0 M NaCl) on the intrinsic viscosity and steady shear flow were evaluated using a central composite rotatable design of experiments and surface response methodology.
26014143	9	44	theme	viscosity	1115:1123	arg1	stability					1089:1097	stability	1089:1097	stability of the apparent viscosity under pH and ionic strength variations	1089:1162	Given the results obtained, FucoPol is proposed as thickening agent for applications in which stability of the apparent viscosity under pH and ionic strength variations is required.
26014143	7	45	theme	Newtonian	754:762	arg1	plateau					764:770	the first Newtonian plateau	744:770	the first Newtonian plateau	744:770	The viscosity of the first Newtonian plateau varied from 0.01 to 2.47 Pas for polymer concentrations from 0.2 to 1.2 wt.
26014143	3	46	theme	0.05-0.50	525:533	arg1	M					535:535	M	535:535	M	535:535	FucoPol's intrinsic and apparent viscosities presented a quite low variation under a wide range of pH (3.5-8.0) and ionic strength (0.05-0.50 M NaCl) values.
26014143	7	47	dep	2.47	792:795	arg1	to					789:790	to	789:790	to	789:790	The viscosity of the first Newtonian plateau varied from 0.01 to 2.47 Pas for polymer concentrations from 0.2 to 1.2 wt.
26014143	0	48	theme	FucoPol	57:63	arg1	studies					12:18	Rheological studies	0:18	Rheological studies of the fucose-rich exopolysaccharide FucoPol	0:63	Rheological studies of the fucose-rich exopolysaccharide FucoPol.
26014143	3	49	theme	M	535:535	arg1	NaCl					537:540	0.05-0.50 M NaCl	525:540	0.05-0.50 M NaCl	525:540	FucoPol's intrinsic and apparent viscosities presented a quite low variation under a wide range of pH (3.5-8.0) and ionic strength (0.05-0.50 M NaCl) values.
26014143	3	49	theme	M	535:535	arg1	strength					515:522	ionic strength	509:522	ionic strength (0.05-0.50 M NaCl)	509:541	FucoPol's intrinsic and apparent viscosities presented a quite low variation under a wide range of pH (3.5-8.0) and ionic strength (0.05-0.50 M NaCl) values.
26014143	1	50	theme	bacterial	111:119	arg1	FucoPol					149:155	FucoPol	149:155	FucoPol	149:155	In this work, the solution properties of the bacterial fucose-rich polysaccharide, FucoPol, were studied.
26014143	1	50	theme	bacterial	111:119	arg1	polysaccharide					133:146	the bacterial fucose-rich polysaccharide	107:146	the bacterial fucose-rich polysaccharide	107:146	In this work, the solution properties of the bacterial fucose-rich polysaccharide, FucoPol, were studied.
26014143	9	51	dep	pH	1131:1132	arg1	variations					1153:1162	variations	1153:1162	variations	1153:1162	Given the results obtained, FucoPol is proposed as thickening agent for applications in which stability of the apparent viscosity under pH and ionic strength variations is required.
26014143	7	52	dep	1.2	840:842	arg1	to					837:838	to	837:838	to	837:838	The viscosity of the first Newtonian plateau varied from 0.01 to 2.47 Pas for polymer concentrations from 0.2 to 1.2 wt.
26014143	1	53	theme	fucose-rich	121:131	arg1	FucoPol					149:155	FucoPol	149:155	FucoPol	149:155	In this work, the solution properties of the bacterial fucose-rich polysaccharide, FucoPol, were studied.
26014143	1	53	theme	fucose-rich	121:131	arg1	polysaccharide					133:146	the bacterial fucose-rich polysaccharide	107:146	the bacterial fucose-rich polysaccharide	107:146	In this work, the solution properties of the bacterial fucose-rich polysaccharide, FucoPol, were studied.
26014143	2	54	theme	pH	186:187	arg1	effect					176:181	The effect	172:181	The effect of pH (3.5-10.0) and ionic strength (0.02-1.0 M NaCl) on the intrinsic viscosity and steady shear flow	172:284	The effect of pH (3.5-10.0) and ionic strength (0.02-1.0 M NaCl) on the intrinsic viscosity and steady shear flow were evaluated using a central composite rotatable design of experiments and surface response methodology.
26014143	2	55	theme	M	229:229	arg1	strength					210:217	ionic strength	204:217	ionic strength (0.02-1.0 M NaCl)	204:235	The effect of pH (3.5-10.0) and ionic strength (0.02-1.0 M NaCl) on the intrinsic viscosity and steady shear flow were evaluated using a central composite rotatable design of experiments and surface response methodology.
26014143	2	55	theme	M	229:229	arg1	NaCl					231:234	0.02-1.0 M NaCl	220:234	0.02-1.0 M NaCl	220:234	The effect of pH (3.5-10.0) and ionic strength (0.02-1.0 M NaCl) on the intrinsic viscosity and steady shear flow were evaluated using a central composite rotatable design of experiments and surface response methodology.
26014143	1	56	theme	polysaccharide	133:146	arg1	properties					93:102	the solution properties	80:102	the solution properties of the bacterial fucose-rich polysaccharide, FucoPol,	80:156	In this work, the solution properties of the bacterial fucose-rich polysaccharide, FucoPol, were studied.
26014143	2	57	theme	0.02-1.0	220:227	arg1	M					229:229	M	229:229	M	229:229	The effect of pH (3.5-10.0) and ionic strength (0.02-1.0 M NaCl) on the intrinsic viscosity and steady shear flow were evaluated using a central composite rotatable design of experiments and surface response methodology.
26014143	2	58	theme	rotatable	327:335	arg1	design					337:342	a central composite rotatable design	307:342	a central composite rotatable design of experiments	307:357	The effect of pH (3.5-10.0) and ionic strength (0.02-1.0 M NaCl) on the intrinsic viscosity and steady shear flow were evaluated using a central composite rotatable design of experiments and surface response methodology.
26014143	8	59	theme	estimated	876:884	arg1	time					897:900	the estimated relaxation time	872:900	the estimated relaxation time	872:900	%, and the dependence of the estimated relaxation time with polymer concentration suggests a large degree of interaction between FucoPol molecules.
26014143	2	60	theme	composite	317:325	arg1	design					337:342	a central composite rotatable design	307:342	a central composite rotatable design of experiments	307:357	The effect of pH (3.5-10.0) and ionic strength (0.02-1.0 M NaCl) on the intrinsic viscosity and steady shear flow were evaluated using a central composite rotatable design of experiments and surface response methodology.
27402833	6	0	with	HIV-1	1018:1022	arg1	potency					1044:1050	∼4-fold higher potency	1029:1050	∼4-fold higher potency than CV-N(P51G), a stabilized version of wild type CV-N	1029:1106	HIV assays showed that Cyt-CVNH is able to inhibit HIV-1 with ∼4-fold higher potency than CV-N(P51G), a stabilized version of wild type CV-N.
27402833	5	1	from	cross-linking	749:761	arg1	solution					812:819	solution	812:819	solution	812:819	However, in contrast to CV-N, no cross-linking and precipitation of the cross-linked species in solution was observed upon Man-9 binding, allowing, for the first time, investigation of the interaction of Man-9 with a member of the CVNH family by NMR.
27402833	1	2	theme	mannose	146:152	arg1	sugars					154:159	numerous high mannose sugars	132:159	numerous high mannose sugars	132:159	The HIV-1 envelope glycoprotein gp120 is heavily glycosylated and bears numerous high mannose sugars.
27402833	4	3	theme	D3	683:684	arg1	glycans					707:713	high mannose glycans	694:713	high mannose glycans	694:713	The Cyt-CVNH structure and glycan recognition profile are similar to those of other CVNH proteins, with each domain specifically binding to Manα(1-2)Manα units on the D1 and D3 arms of high mannose glycans.
27402833	4	3	theme	D3	683:684	arg1	arms					686:689	the D1 and D3 arms	672:689	the D1 and D3 arms of high mannose glycans	672:713	The Cyt-CVNH structure and glycan recognition profile are similar to those of other CVNH proteins, with each domain specifically binding to Manα(1-2)Manα units on the D1 and D3 arms of high mannose glycans.
27402833	5	4	theme	cross-linked	788:799	arg1	species					801:807	the cross-linked species	784:807	the cross-linked species	784:807	However, in contrast to CV-N, no cross-linking and precipitation of the cross-linked species in solution was observed upon Man-9 binding, allowing, for the first time, investigation of the interaction of Man-9 with a member of the CVNH family by NMR.
27402833	4	5	with	proteins	598:605	arg1	domain					618:623	each domain	613:623	each domain specifically binding to Manα(1-2)Manα units on the D1 and D3 arms of high mannose glycans	613:713	The Cyt-CVNH structure and glycan recognition profile are similar to those of other CVNH proteins, with each domain specifically binding to Manα(1-2)Manα units on the D1 and D3 arms of high mannose glycans.
27402833	1	6	gly	glycoprotein	79:90	arg1	glycoprotein					79:90	The HIV-1 envelope glycoprotein gp120	60:96	The HIV-1 envelope glycoprotein gp120	60:96	The HIV-1 envelope glycoprotein gp120 is heavily glycosylated and bears numerous high mannose sugars.
27402833	5	7	dep	CV-N	740:743	arg1	contrast					728:735	contrast	728:735	contrast	728:735	However, in contrast to CV-N, no cross-linking and precipitation of the cross-linked species in solution was observed upon Man-9 binding, allowing, for the first time, investigation of the interaction of Man-9 with a member of the CVNH family by NMR.
27402833	6	8	theme	higher	1037:1042	arg1	potency					1044:1050	∼4-fold higher potency	1029:1050	∼4-fold higher potency than CV-N(P51G), a stabilized version of wild type CV-N	1029:1106	HIV assays showed that Cyt-CVNH is able to inhibit HIV-1 with ∼4-fold higher potency than CV-N(P51G), a stabilized version of wild type CV-N.
27402833	4	9	theme	recognition	543:553	arg1	profile					555:561	glycan recognition profile	536:561	glycan recognition profile	536:561	The Cyt-CVNH structure and glycan recognition profile are similar to those of other CVNH proteins, with each domain specifically binding to Manα(1-2)Manα units on the D1 and D3 arms of high mannose glycans.
27402833	4	10	theme	D1	676:677	arg1	glycans					707:713	high mannose glycans	694:713	high mannose glycans	694:713	The Cyt-CVNH structure and glycan recognition profile are similar to those of other CVNH proteins, with each domain specifically binding to Manα(1-2)Manα units on the D1 and D3 arms of high mannose glycans.
27402833	4	10	theme	D1	676:677	arg1	arms					686:689	the D1 and D3 arms	672:689	the D1 and D3 arms of high mannose glycans	672:713	The Cyt-CVNH structure and glycan recognition profile are similar to those of other CVNH proteins, with each domain specifically binding to Manα(1-2)Manα units on the D1 and D3 arms of high mannose glycans.
27402833	6	11	theme	type	1098:1101	arg1	CV-N					1057:1060	CV-N	1057:1060	CV-N(P51G)	1057:1066	HIV assays showed that Cyt-CVNH is able to inhibit HIV-1 with ∼4-fold higher potency than CV-N(P51G), a stabilized version of wild type CV-N.
27402833	6	11	theme	type	1098:1101	arg1	version					1082:1088	a stabilized version	1069:1088	a stabilized version of wild type CV-N	1069:1106	HIV assays showed that Cyt-CVNH is able to inhibit HIV-1 with ∼4-fold higher potency than CV-N(P51G), a stabilized version of wild type CV-N.
27402833	2	12	theme	target	326:331	arg1	cell					333:336	the target cell	322:336	the target cell	322:336	These sugars can serve as targets for HIV-inactivating compounds, such as antibodies and lectins, which bind to the glycans and interfere with viral entry into the target cell.
27402833	4	13	theme	glycan	536:541	arg1	profile					555:561	glycan recognition profile	536:561	glycan recognition profile	536:561	The Cyt-CVNH structure and glycan recognition profile are similar to those of other CVNH proteins, with each domain specifically binding to Manα(1-2)Manα units on the D1 and D3 arms of high mannose glycans.
27402833	6	14	theme	wild	1093:1096	arg1	type					1098:1101	wild type CV-N	1093:1106	wild type CV-N	1093:1106	HIV assays showed that Cyt-CVNH is able to inhibit HIV-1 with ∼4-fold higher potency than CV-N(P51G), a stabilized version of wild type CV-N.
27402833	5	15	theme	family	952:957	arg1	member					933:938	a member	931:938	a member of the CVNH family	931:957	However, in contrast to CV-N, no cross-linking and precipitation of the cross-linked species in solution was observed upon Man-9 binding, allowing, for the first time, investigation of the interaction of Man-9 with a member of the CVNH family by NMR.
27402833	0	16	theme	New	34:36	arg1	Homolog					51:57	a New Cyanovirin-N Homolog	32:57	a New Cyanovirin-N Homolog	32:57	Structure and Glycan Binding of a New Cyanovirin-N Homolog.
27402833	1	17	theme	HIV-1	64:68	arg1	gp120					92:96	The HIV-1 envelope glycoprotein gp120	60:96	The HIV-1 envelope glycoprotein gp120	60:96	The HIV-1 envelope glycoprotein gp120 is heavily glycosylated and bears numerous high mannose sugars.
27402833	3	18	from	Cyanothece	445:454	arg1	lectin					414:419	a recently identified lectin	392:419	a recently identified lectin from the cyanobacterium Cyanothece(7424)	392:460	We determined the 1.6 Å x-ray structure of Cyt-CVNH, a recently identified lectin from the cyanobacterium Cyanothece(7424), and elucidated its glycan specificity by NMR.
27402833	3	18	from	Cyanothece	445:454	arg1	Cyt-CVNH					382:389	Cyt-CVNH	382:389	Cyt-CVNH	382:389	We determined the 1.6 Å x-ray structure of Cyt-CVNH, a recently identified lectin from the cyanobacterium Cyanothece(7424), and elucidated its glycan specificity by NMR.
27402833	1	19	theme	envelope	70:77	arg1	gp120					92:96	The HIV-1 envelope glycoprotein gp120	60:96	The HIV-1 envelope glycoprotein gp120	60:96	The HIV-1 envelope glycoprotein gp120 is heavily glycosylated and bears numerous high mannose sugars.
27402833	3	20	theme	Cyt-CVNH	382:389	arg1	structure					369:377	the 1.6 Å x-ray structure	353:377	the 1.6 Å x-ray structure of Cyt-CVNH, a recently identified lectin from the cyanobacterium Cyanothece(7424),	353:461	We determined the 1.6 Å x-ray structure of Cyt-CVNH, a recently identified lectin from the cyanobacterium Cyanothece(7424), and elucidated its glycan specificity by NMR.
27402833	6	21	theme	stabilized	1071:1080	arg1	CV-N					1057:1060	CV-N	1057:1060	CV-N(P51G)	1057:1066	HIV assays showed that Cyt-CVNH is able to inhibit HIV-1 with ∼4-fold higher potency than CV-N(P51G), a stabilized version of wild type CV-N.
27402833	6	21	theme	stabilized	1071:1080	arg1	version					1082:1088	a stabilized version	1069:1088	a stabilized version of wild type CV-N	1069:1106	HIV assays showed that Cyt-CVNH is able to inhibit HIV-1 with ∼4-fold higher potency than CV-N(P51G), a stabilized version of wild type CV-N.
27402833	4	22	from	units	663:667	arg1	glycans					707:713	high mannose glycans	694:713	high mannose glycans	694:713	The Cyt-CVNH structure and glycan recognition profile are similar to those of other CVNH proteins, with each domain specifically binding to Manα(1-2)Manα units on the D1 and D3 arms of high mannose glycans.
27402833	4	22	from	units	663:667	arg1	arms					686:689	the D1 and D3 arms	672:689	the D1 and D3 arms of high mannose glycans	672:713	The Cyt-CVNH structure and glycan recognition profile are similar to those of other CVNH proteins, with each domain specifically binding to Manα(1-2)Manα units on the D1 and D3 arms of high mannose glycans.
27402833	1	23	theme	glycoprotein	79:90	arg1	gp120					92:96	The HIV-1 envelope glycoprotein gp120	60:96	The HIV-1 envelope glycoprotein gp120	60:96	The HIV-1 envelope glycoprotein gp120 is heavily glycosylated and bears numerous high mannose sugars.
27402833	4	24	theme	Manα	658:661	arg1	units					663:667	Manα(1-2)Manα units	649:667	Manα(1-2)Manα units on the D1 and D3 arms of high mannose glycans	649:713	The Cyt-CVNH structure and glycan recognition profile are similar to those of other CVNH proteins, with each domain specifically binding to Manα(1-2)Manα units on the D1 and D3 arms of high mannose glycans.
27402833	1	25	gly	glycosylated	109:120	arg1	gp120					92:96	The HIV-1 envelope glycoprotein gp120	60:96	The HIV-1 envelope glycoprotein gp120	60:96	The HIV-1 envelope glycoprotein gp120 is heavily glycosylated and bears numerous high mannose sugars.
27402833	0	26	theme	Glycan	14:19	arg1	Binding					21:27	Glycan Binding	14:27	Glycan Binding	14:27	Structure and Glycan Binding of a New Cyanovirin-N Homolog.
27402833	4	27	theme	Cyt-CVNH	513:520	arg1	structure					522:530	The Cyt-CVNH structure	509:530	The Cyt-CVNH structure	509:530	The Cyt-CVNH structure and glycan recognition profile are similar to those of other CVNH proteins, with each domain specifically binding to Manα(1-2)Manα units on the D1 and D3 arms of high mannose glycans.
27402833	4	27	theme	Cyt-CVNH	513:520	arg1	similar					567:573	similar	567:573	similar	567:573	The Cyt-CVNH structure and glycan recognition profile are similar to those of other CVNH proteins, with each domain specifically binding to Manα(1-2)Manα units on the D1 and D3 arms of high mannose glycans.
27402833	5	28	theme	Man-9	839:843	arg1	binding					845:851	Man-9 binding	839:851	Man-9 binding	839:851	However, in contrast to CV-N, no cross-linking and precipitation of the cross-linked species in solution was observed upon Man-9 binding, allowing, for the first time, investigation of the interaction of Man-9 with a member of the CVNH family by NMR.
27402833	5	29	theme	species	801:807	arg1	cross-linking					749:761	cross-linking	749:761	cross-linking	749:761	However, in contrast to CV-N, no cross-linking and precipitation of the cross-linked species in solution was observed upon Man-9 binding, allowing, for the first time, investigation of the interaction of Man-9 with a member of the CVNH family by NMR.
27402833	5	29	theme	species	801:807	arg1	precipitation					767:779	precipitation	767:779	precipitation	767:779	However, in contrast to CV-N, no cross-linking and precipitation of the cross-linked species in solution was observed upon Man-9 binding, allowing, for the first time, investigation of the interaction of Man-9 with a member of the CVNH family by NMR.
27402833	6	30	theme	HIV	967:969	arg1	assays					971:976	HIV assays	967:976	HIV assays	967:976	HIV assays showed that Cyt-CVNH is able to inhibit HIV-1 with ∼4-fold higher potency than CV-N(P51G), a stabilized version of wild type CV-N.
27402833	4	31	theme	CVNH	593:596	arg1	proteins					598:605	other CVNH proteins	587:605	other CVNH proteins	587:605	The Cyt-CVNH structure and glycan recognition profile are similar to those of other CVNH proteins, with each domain specifically binding to Manα(1-2)Manα units on the D1 and D3 arms of high mannose glycans.
27402833	3	32	theme	identified	403:412	arg1	lectin					414:419	a recently identified lectin	392:419	a recently identified lectin from the cyanobacterium Cyanothece(7424)	392:460	We determined the 1.6 Å x-ray structure of Cyt-CVNH, a recently identified lectin from the cyanobacterium Cyanothece(7424), and elucidated its glycan specificity by NMR.
27402833	3	32	theme	identified	403:412	arg1	Cyt-CVNH					382:389	Cyt-CVNH	382:389	Cyt-CVNH	382:389	We determined the 1.6 Å x-ray structure of Cyt-CVNH, a recently identified lectin from the cyanobacterium Cyanothece(7424), and elucidated its glycan specificity by NMR.
27402833	7	33	theme	valuable	1146:1153	arg1	lectin					1155:1160	a valuable lectin	1144:1160	a valuable lectin for potential microbicidal use	1144:1191	Therefore, Cyt-CVNH may qualify as a valuable lectin for potential microbicidal use.
27402833	5	34	theme	CVNH	947:950	arg1	family					952:957	the CVNH family	943:957	the CVNH family	943:957	However, in contrast to CV-N, no cross-linking and precipitation of the cross-linked species in solution was observed upon Man-9 binding, allowing, for the first time, investigation of the interaction of Man-9 with a member of the CVNH family by NMR.
27402833	4	35	theme	other	587:591	arg1	proteins					598:605	other CVNH proteins	587:605	other CVNH proteins	587:605	The Cyt-CVNH structure and glycan recognition profile are similar to those of other CVNH proteins, with each domain specifically binding to Manα(1-2)Manα units on the D1 and D3 arms of high mannose glycans.
27402833	3	36	theme	Å	361:361	arg1	structure					369:377	the 1.6 Å x-ray structure	353:377	the 1.6 Å x-ray structure of Cyt-CVNH, a recently identified lectin from the cyanobacterium Cyanothece(7424),	353:461	We determined the 1.6 Å x-ray structure of Cyt-CVNH, a recently identified lectin from the cyanobacterium Cyanothece(7424), and elucidated its glycan specificity by NMR.
27402833	5	37	with	interaction	905:915	arg1	member					933:938	a member	931:938	a member of the CVNH family	931:957	However, in contrast to CV-N, no cross-linking and precipitation of the cross-linked species in solution was observed upon Man-9 binding, allowing, for the first time, investigation of the interaction of Man-9 with a member of the CVNH family by NMR.
27402833	0	38	theme	Homolog	51:57	arg1	Structure					0:8	Structure	0:8	Structure	0:8	Structure and Glycan Binding of a New Cyanovirin-N Homolog.
27402833	0	38	theme	Homolog	51:57	arg1	Binding					21:27	Glycan Binding	14:27	Glycan Binding	14:27	Structure and Glycan Binding of a New Cyanovirin-N Homolog.
27402833	5	39	theme	interaction	905:915	arg1	investigation					884:896	investigation	884:896	investigation of the interaction of Man-9 with a member of the CVNH family	884:957	However, in contrast to CV-N, no cross-linking and precipitation of the cross-linked species in solution was observed upon Man-9 binding, allowing, for the first time, investigation of the interaction of Man-9 with a member of the CVNH family by NMR.
27402833	3	40	theme	x-ray	363:367	arg1	structure					369:377	the 1.6 Å x-ray structure	353:377	the 1.6 Å x-ray structure of Cyt-CVNH, a recently identified lectin from the cyanobacterium Cyanothece(7424),	353:461	We determined the 1.6 Å x-ray structure of Cyt-CVNH, a recently identified lectin from the cyanobacterium Cyanothece(7424), and elucidated its glycan specificity by NMR.
27402833	5	41	link	cross-linked	788:799	arg1	species					801:807	the cross-linked species	784:807	the cross-linked species	784:807	However, in contrast to CV-N, no cross-linking and precipitation of the cross-linked species in solution was observed upon Man-9 binding, allowing, for the first time, investigation of the interaction of Man-9 with a member of the CVNH family by NMR.
27402833	5	42	theme	Man-9	920:924	arg1	interaction					905:915	the interaction	901:915	the interaction of Man-9 with a member of the CVNH family	901:957	However, in contrast to CV-N, no cross-linking and precipitation of the cross-linked species in solution was observed upon Man-9 binding, allowing, for the first time, investigation of the interaction of Man-9 with a member of the CVNH family by NMR.
27402833	2	43	theme	HIV-inactivating	200:215	arg1	antibodies					236:245	antibodies	236:245	antibodies	236:245	These sugars can serve as targets for HIV-inactivating compounds, such as antibodies and lectins, which bind to the glycans and interfere with viral entry into the target cell.
27402833	2	43	theme	HIV-inactivating	200:215	arg1	compounds					217:225	HIV-inactivating compounds	200:225	HIV-inactivating compounds	200:225	These sugars can serve as targets for HIV-inactivating compounds, such as antibodies and lectins, which bind to the glycans and interfere with viral entry into the target cell.
27402833	2	43	theme	HIV-inactivating	200:215	arg1	lectins					251:257	lectins	251:257	lectins	251:257	These sugars can serve as targets for HIV-inactivating compounds, such as antibodies and lectins, which bind to the glycans and interfere with viral entry into the target cell.
27402833	4	44	theme	Manα	649:652	arg1	units					663:667	Manα(1-2)Manα units	649:667	Manα(1-2)Manα units on the D1 and D3 arms of high mannose glycans	649:713	The Cyt-CVNH structure and glycan recognition profile are similar to those of other CVNH proteins, with each domain specifically binding to Manα(1-2)Manα units on the D1 and D3 arms of high mannose glycans.
27402833	7	45	theme	potential	1166:1174	arg1	use					1189:1191	potential microbicidal use	1166:1191	potential microbicidal use	1166:1191	Therefore, Cyt-CVNH may qualify as a valuable lectin for potential microbicidal use.
27402833	5	46	theme	first	872:876	arg1	time					878:881	the first time	868:881	the first time	868:881	However, in contrast to CV-N, no cross-linking and precipitation of the cross-linked species in solution was observed upon Man-9 binding, allowing, for the first time, investigation of the interaction of Man-9 with a member of the CVNH family by NMR.
27402833	4	47	theme	high	694:697	arg1	glycans					707:713	high mannose glycans	694:713	high mannose glycans	694:713	The Cyt-CVNH structure and glycan recognition profile are similar to those of other CVNH proteins, with each domain specifically binding to Manα(1-2)Manα units on the D1 and D3 arms of high mannose glycans.
27402833	2	48	theme	viral	305:309	arg1	entry					311:315	viral entry	305:315	viral entry into the target cell	305:336	These sugars can serve as targets for HIV-inactivating compounds, such as antibodies and lectins, which bind to the glycans and interfere with viral entry into the target cell.
27402833	3	49	theme	glycan	482:487	arg1	specificity					489:499	its glycan specificity	478:499	its glycan specificity	478:499	We determined the 1.6 Å x-ray structure of Cyt-CVNH, a recently identified lectin from the cyanobacterium Cyanothece(7424), and elucidated its glycan specificity by NMR.
27402833	7	50	theme	microbicidal	1176:1187	arg1	use					1189:1191	potential microbicidal use	1166:1191	potential microbicidal use	1166:1191	Therefore, Cyt-CVNH may qualify as a valuable lectin for potential microbicidal use.
27402833	3	51	theme	cyanobacterium	430:443	arg1	7424					456:459	7424	456:459	7424	456:459	We determined the 1.6 Å x-ray structure of Cyt-CVNH, a recently identified lectin from the cyanobacterium Cyanothece(7424), and elucidated its glycan specificity by NMR.
27402833	3	51	theme	cyanobacterium	430:443	arg1	Cyanothece					445:454	the cyanobacterium Cyanothece	426:454	the cyanobacterium Cyanothece(7424)	426:460	We determined the 1.6 Å x-ray structure of Cyt-CVNH, a recently identified lectin from the cyanobacterium Cyanothece(7424), and elucidated its glycan specificity by NMR.
27402833	4	52	theme	glycans	707:713	arg1	glycans					707:713	high mannose glycans	694:713	high mannose glycans	694:713	The Cyt-CVNH structure and glycan recognition profile are similar to those of other CVNH proteins, with each domain specifically binding to Manα(1-2)Manα units on the D1 and D3 arms of high mannose glycans.
27402833	4	52	theme	glycans	707:713	arg1	arms					686:689	the D1 and D3 arms	672:689	the D1 and D3 arms of high mannose glycans	672:713	The Cyt-CVNH structure and glycan recognition profile are similar to those of other CVNH proteins, with each domain specifically binding to Manα(1-2)Manα units on the D1 and D3 arms of high mannose glycans.
27402833	5	53	from	precipitation	767:779	arg1	solution					812:819	solution	812:819	solution	812:819	However, in contrast to CV-N, no cross-linking and precipitation of the cross-linked species in solution was observed upon Man-9 binding, allowing, for the first time, investigation of the interaction of Man-9 with a member of the CVNH family by NMR.
27402833	1	54	theme	numerous	132:139	arg1	sugars					154:159	numerous high mannose sugars	132:159	numerous high mannose sugars	132:159	The HIV-1 envelope glycoprotein gp120 is heavily glycosylated and bears numerous high mannose sugars.
27402833	0	55	theme	Cyanovirin-N	38:49	arg1	Homolog					51:57	a New Cyanovirin-N Homolog	32:57	a New Cyanovirin-N Homolog	32:57	Structure and Glycan Binding of a New Cyanovirin-N Homolog.
27402833	1	56	contain	bears	126:130	arg2	sugars					154:159	numerous high mannose sugars	132:159	numerous high mannose sugars	132:159	The HIV-1 envelope glycoprotein gp120 is heavily glycosylated and bears numerous high mannose sugars.
27402833	1	56	contain	bears	126:130	arg1	gp120					92:96	The HIV-1 envelope glycoprotein gp120	60:96	The HIV-1 envelope glycoprotein gp120	60:96	The HIV-1 envelope glycoprotein gp120 is heavily glycosylated and bears numerous high mannose sugars.
27402833	4	57	theme	mannose	699:705	arg1	glycans					707:713	high mannose glycans	694:713	high mannose glycans	694:713	The Cyt-CVNH structure and glycan recognition profile are similar to those of other CVNH proteins, with each domain specifically binding to Manα(1-2)Manα units on the D1 and D3 arms of high mannose glycans.
27402833	1	58	theme	high	141:144	arg1	sugars					154:159	numerous high mannose sugars	132:159	numerous high mannose sugars	132:159	The HIV-1 envelope glycoprotein gp120 is heavily glycosylated and bears numerous high mannose sugars.
23818238	0	0	theme	alginate	90:97	arg1	films					110:114	chitosan/sodium alginate multilayer films	74:114	chitosan/sodium alginate multilayer films	74:114	Structural and optical properties of Cu-doped ZnS nanoparticles formed in chitosan/sodium alginate multilayer films.
23818238	1	1	theme	natural	258:264	arg1	alginate					303:310	sodium alginate	296:310	sodium alginate	296:310	Chitosan/alginate multilayers were fabricated using a spin-coating method, and ZnS:Cu nanoparticles were generated within the network of two natural polysaccharides, chitosan and sodium alginate.
23818238	1	1	theme	natural	258:264	arg1	polysaccharides					266:280	two natural polysaccharides	254:280	two natural polysaccharides	254:280	Chitosan/alginate multilayers were fabricated using a spin-coating method, and ZnS:Cu nanoparticles were generated within the network of two natural polysaccharides, chitosan and sodium alginate.
23818238	1	1	theme	natural	258:264	arg1	chitosan					283:290	chitosan	283:290	chitosan	283:290	Chitosan/alginate multilayers were fabricated using a spin-coating method, and ZnS:Cu nanoparticles were generated within the network of two natural polysaccharides, chitosan and sodium alginate.
23818238	0	2	theme	chitosan/sodium	74:88	arg1	films					110:114	chitosan/sodium alginate multilayer films	74:114	chitosan/sodium alginate multilayer films	74:114	Structural and optical properties of Cu-doped ZnS nanoparticles formed in chitosan/sodium alginate multilayer films.
23818238	4	3	dep	nanoparticles	743:755	arg1	shifted					766:772	shifted	766:772	nanoparticles slightly shifted to longer wavelengths with increasing Cu ion concentrations	743:832	UV-vis spectra indicate a large quantum size effect and the absorption edge for the ZnS:Cu nanoparticles slightly shifted to longer wavelengths with increasing Cu ion concentrations.
23818238	1	4	theme	polysaccharides	266:280	arg1	network					243:249	the network	239:249	the network of two natural polysaccharides, chitosan and sodium alginate	239:310	Chitosan/alginate multilayers were fabricated using a spin-coating method, and ZnS:Cu nanoparticles were generated within the network of two natural polysaccharides, chitosan and sodium alginate.
23818238	3	5	with	nanoparticles	571:583	arg1	of ~ 3 nm					614:622	an average crystal size of ~ 3 nm	590:622	an average crystal size of ~ 3 nm	590:622	The results showed that cubic zinc blende-structured ZnS:Cu nanoparticles with an average crystal size of ~ 3 nm were uniformly distributed.
23818238	3	6	theme	cubic	535:539	arg1	nanoparticles					571:583	cubic zinc blende-structured ZnS:Cu nanoparticles	535:583	cubic zinc blende-structured ZnS:Cu nanoparticles with an average crystal size of ~ 3 nm	535:622	The results showed that cubic zinc blende-structured ZnS:Cu nanoparticles with an average crystal size of ~ 3 nm were uniformly distributed.
23818238	3	7	theme	ZnS	564:566	arg1	nanoparticles					571:583	cubic zinc blende-structured ZnS:Cu nanoparticles	535:583	cubic zinc blende-structured ZnS:Cu nanoparticles with an average crystal size of ~ 3 nm	535:622	The results showed that cubic zinc blende-structured ZnS:Cu nanoparticles with an average crystal size of ~ 3 nm were uniformly distributed.
23818238	0	8	theme	multilayer	99:108	arg1	films					110:114	chitosan/sodium alginate multilayer films	74:114	chitosan/sodium alginate multilayer films	74:114	Structural and optical properties of Cu-doped ZnS nanoparticles formed in chitosan/sodium alginate multilayer films.
23818238	3	9	theme	zinc	541:544	arg1	nanoparticles					571:583	cubic zinc blende-structured ZnS:Cu nanoparticles	535:583	cubic zinc blende-structured ZnS:Cu nanoparticles with an average crystal size of ~ 3 nm	535:622	The results showed that cubic zinc blende-structured ZnS:Cu nanoparticles with an average crystal size of ~ 3 nm were uniformly distributed.
23818238	4	10	theme	absorption	712:721	arg1	edge					723:726	the absorption edge	708:726	the absorption edge	708:726	UV-vis spectra indicate a large quantum size effect and the absorption edge for the ZnS:Cu nanoparticles slightly shifted to longer wavelengths with increasing Cu ion concentrations.
23818238	6	11	theme	surface	1032:1038	arg1	height					1048:1053	a surface average height	1030:1053	a surface average height of 25 nm	1030:1062	The ZnS:Cu nanoparticles form and are distributed uniformly in the composite multilayer films with a surface average height of 25 nm.
23818238	4	12	theme	large	678:682	arg1	effect					697:702	a large quantum size effect	676:702	a large quantum size effect	676:702	UV-vis spectra indicate a large quantum size effect and the absorption edge for the ZnS:Cu nanoparticles slightly shifted to longer wavelengths with increasing Cu ion concentrations.
23818238	5	13	theme	Cu-doped	864:871	arg1	nanoparticles					877:889	the Cu-doped ZnS nanoparticles	860:889	the Cu-doped ZnS nanoparticles	860:889	The photoluminescence of the Cu-doped ZnS nanoparticles reached a maximum at a 1% doping level.
23818238	2	14	theme	atomic	480:485	arg1	AFM					505:507	AFM	505:507	AFM	505:507	The synthesized nanoparticles were characterized using an X-ray diffractometer (XRD), transmission electron microscopy (TEM), energy dispersive spectroscopy (EDS) and atomic force microscopy (AFM).
23818238	2	14	theme	atomic	480:485	arg1	microscopy					493:502	atomic force microscopy	480:502	atomic force microscopy (AFM)	480:508	The synthesized nanoparticles were characterized using an X-ray diffractometer (XRD), transmission electron microscopy (TEM), energy dispersive spectroscopy (EDS) and atomic force microscopy (AFM).
23818238	2	14	theme	atomic	480:485	arg1	diffractometer					377:390	an X-ray diffractometer	368:390	an X-ray diffractometer (XRD)	368:396	The synthesized nanoparticles were characterized using an X-ray diffractometer (XRD), transmission electron microscopy (TEM), energy dispersive spectroscopy (EDS) and atomic force microscopy (AFM).
23818238	2	15	theme	electron	412:419	arg1	TEM					433:435	TEM	433:435	TEM	433:435	The synthesized nanoparticles were characterized using an X-ray diffractometer (XRD), transmission electron microscopy (TEM), energy dispersive spectroscopy (EDS) and atomic force microscopy (AFM).
23818238	2	15	theme	electron	412:419	arg1	microscopy					421:430	transmission electron microscopy	399:430	transmission electron microscopy (TEM)	399:436	The synthesized nanoparticles were characterized using an X-ray diffractometer (XRD), transmission electron microscopy (TEM), energy dispersive spectroscopy (EDS) and atomic force microscopy (AFM).
23818238	2	15	theme	electron	412:419	arg1	diffractometer					377:390	an X-ray diffractometer	368:390	an X-ray diffractometer (XRD)	368:396	The synthesized nanoparticles were characterized using an X-ray diffractometer (XRD), transmission electron microscopy (TEM), energy dispersive spectroscopy (EDS) and atomic force microscopy (AFM).
23818238	2	16	theme	force	487:491	arg1	AFM					505:507	AFM	505:507	AFM	505:507	The synthesized nanoparticles were characterized using an X-ray diffractometer (XRD), transmission electron microscopy (TEM), energy dispersive spectroscopy (EDS) and atomic force microscopy (AFM).
23818238	2	16	theme	force	487:491	arg1	microscopy					493:502	atomic force microscopy	480:502	atomic force microscopy (AFM)	480:508	The synthesized nanoparticles were characterized using an X-ray diffractometer (XRD), transmission electron microscopy (TEM), energy dispersive spectroscopy (EDS) and atomic force microscopy (AFM).
23818238	2	16	theme	force	487:491	arg1	diffractometer					377:390	an X-ray diffractometer	368:390	an X-ray diffractometer (XRD)	368:396	The synthesized nanoparticles were characterized using an X-ray diffractometer (XRD), transmission electron microscopy (TEM), energy dispersive spectroscopy (EDS) and atomic force microscopy (AFM).
23818238	2	17	theme	transmission	399:410	arg1	TEM					433:435	TEM	433:435	TEM	433:435	The synthesized nanoparticles were characterized using an X-ray diffractometer (XRD), transmission electron microscopy (TEM), energy dispersive spectroscopy (EDS) and atomic force microscopy (AFM).
23818238	2	17	theme	transmission	399:410	arg1	microscopy					421:430	transmission electron microscopy	399:430	transmission electron microscopy (TEM)	399:436	The synthesized nanoparticles were characterized using an X-ray diffractometer (XRD), transmission electron microscopy (TEM), energy dispersive spectroscopy (EDS) and atomic force microscopy (AFM).
23818238	2	17	theme	transmission	399:410	arg1	diffractometer					377:390	an X-ray diffractometer	368:390	an X-ray diffractometer (XRD)	368:396	The synthesized nanoparticles were characterized using an X-ray diffractometer (XRD), transmission electron microscopy (TEM), energy dispersive spectroscopy (EDS) and atomic force microscopy (AFM).
23818238	0	18	theme	Structural	0:9	arg1	properties					23:32	Structural and optical properties	0:32	Structural and optical properties of Cu-doped ZnS nanoparticles	0:62	Structural and optical properties of Cu-doped ZnS nanoparticles formed in chitosan/sodium alginate multilayer films.
23818238	3	19	theme	Cu	568:569	arg1	nanoparticles					571:583	cubic zinc blende-structured ZnS:Cu nanoparticles	535:583	cubic zinc blende-structured ZnS:Cu nanoparticles with an average crystal size of ~ 3 nm	535:622	The results showed that cubic zinc blende-structured ZnS:Cu nanoparticles with an average crystal size of ~ 3 nm were uniformly distributed.
23818238	1	20	theme	Cu	200:201	arg1	nanoparticles					203:215	ZnS:Cu nanoparticles	196:215	ZnS:Cu nanoparticles	196:215	Chitosan/alginate multilayers were fabricated using a spin-coating method, and ZnS:Cu nanoparticles were generated within the network of two natural polysaccharides, chitosan and sodium alginate.
23818238	2	21	theme	dispersive	446:455	arg1	spectroscopy					457:468	energy dispersive spectroscopy	439:468	energy dispersive spectroscopy (EDS)	439:474	The synthesized nanoparticles were characterized using an X-ray diffractometer (XRD), transmission electron microscopy (TEM), energy dispersive spectroscopy (EDS) and atomic force microscopy (AFM).
23818238	2	21	theme	dispersive	446:455	arg1	diffractometer					377:390	an X-ray diffractometer	368:390	an X-ray diffractometer (XRD)	368:396	The synthesized nanoparticles were characterized using an X-ray diffractometer (XRD), transmission electron microscopy (TEM), energy dispersive spectroscopy (EDS) and atomic force microscopy (AFM).
23818238	2	21	theme	dispersive	446:455	arg1	EDS					471:473	EDS	471:473	EDS	471:473	The synthesized nanoparticles were characterized using an X-ray diffractometer (XRD), transmission electron microscopy (TEM), energy dispersive spectroscopy (EDS) and atomic force microscopy (AFM).
23818238	1	22	theme	sodium	296:301	arg1	alginate					303:310	sodium alginate	296:310	sodium alginate	296:310	Chitosan/alginate multilayers were fabricated using a spin-coating method, and ZnS:Cu nanoparticles were generated within the network of two natural polysaccharides, chitosan and sodium alginate.
23818238	1	22	theme	sodium	296:301	arg1	polysaccharides					266:280	two natural polysaccharides	254:280	two natural polysaccharides	254:280	Chitosan/alginate multilayers were fabricated using a spin-coating method, and ZnS:Cu nanoparticles were generated within the network of two natural polysaccharides, chitosan and sodium alginate.
23818238	2	23	theme	synthesized	317:327	arg1	nanoparticles					329:341	The synthesized nanoparticles	313:341	The synthesized nanoparticles	313:341	The synthesized nanoparticles were characterized using an X-ray diffractometer (XRD), transmission electron microscopy (TEM), energy dispersive spectroscopy (EDS) and atomic force microscopy (AFM).
23818238	6	24	theme	25 nm	1058:1062	arg1	height					1048:1053	a surface average height	1030:1053	a surface average height of 25 nm	1030:1062	The ZnS:Cu nanoparticles form and are distributed uniformly in the composite multilayer films with a surface average height of 25 nm.
23818238	6	25	theme	average	1040:1046	arg1	height					1048:1053	a surface average height	1030:1053	a surface average height of 25 nm	1030:1062	The ZnS:Cu nanoparticles form and are distributed uniformly in the composite multilayer films with a surface average height of 25 nm.
23818238	2	26	theme	energy	439:444	arg1	spectroscopy					457:468	energy dispersive spectroscopy	439:468	energy dispersive spectroscopy (EDS)	439:474	The synthesized nanoparticles were characterized using an X-ray diffractometer (XRD), transmission electron microscopy (TEM), energy dispersive spectroscopy (EDS) and atomic force microscopy (AFM).
23818238	2	26	theme	energy	439:444	arg1	diffractometer					377:390	an X-ray diffractometer	368:390	an X-ray diffractometer (XRD)	368:396	The synthesized nanoparticles were characterized using an X-ray diffractometer (XRD), transmission electron microscopy (TEM), energy dispersive spectroscopy (EDS) and atomic force microscopy (AFM).
23818238	2	26	theme	energy	439:444	arg1	EDS					471:473	EDS	471:473	EDS	471:473	The synthesized nanoparticles were characterized using an X-ray diffractometer (XRD), transmission electron microscopy (TEM), energy dispersive spectroscopy (EDS) and atomic force microscopy (AFM).
23818238	1	27	theme	Chitosan/alginate	117:133	arg1	multilayers					135:145	Chitosan/alginate multilayers	117:145	Chitosan/alginate multilayers	117:145	Chitosan/alginate multilayers were fabricated using a spin-coating method, and ZnS:Cu nanoparticles were generated within the network of two natural polysaccharides, chitosan and sodium alginate.
23818238	5	28	theme	ZnS	873:875	arg1	nanoparticles					877:889	the Cu-doped ZnS nanoparticles	860:889	the Cu-doped ZnS nanoparticles	860:889	The photoluminescence of the Cu-doped ZnS nanoparticles reached a maximum at a 1% doping level.
23818238	0	29	theme	Cu-doped	37:44	arg1	nanoparticles					50:62	Cu-doped ZnS nanoparticles	37:62	Cu-doped ZnS nanoparticles	37:62	Structural and optical properties of Cu-doped ZnS nanoparticles formed in chitosan/sodium alginate multilayer films.
23818238	6	30	theme	Cu	939:940	arg1	nanoparticles					942:954	Cu nanoparticles	939:954	Cu nanoparticles	939:954	The ZnS:Cu nanoparticles form and are distributed uniformly in the composite multilayer films with a surface average height of 25 nm.
23818238	4	31	theme	ion	815:817	arg1	concentrations					819:832	Cu ion concentrations	812:832	Cu ion concentrations	812:832	UV-vis spectra indicate a large quantum size effect and the absorption edge for the ZnS:Cu nanoparticles slightly shifted to longer wavelengths with increasing Cu ion concentrations.
23818238	4	32	theme	UV-vis	652:657	arg1	spectra					659:665	UV-vis spectra	652:665	UV-vis spectra	652:665	UV-vis spectra indicate a large quantum size effect and the absorption edge for the ZnS:Cu nanoparticles slightly shifted to longer wavelengths with increasing Cu ion concentrations.
23818238	5	33	theme	nanoparticles	877:889	arg1	photoluminescence					839:855	The photoluminescence	835:855	The photoluminescence of the Cu-doped ZnS nanoparticles	835:889	The photoluminescence of the Cu-doped ZnS nanoparticles reached a maximum at a 1% doping level.
23818238	3	34	theme	average	593:599	arg1	size					609:612	an average crystal size	590:612	an average crystal size of ~ 3 nm	590:622	The results showed that cubic zinc blende-structured ZnS:Cu nanoparticles with an average crystal size of ~ 3 nm were uniformly distributed.
23818238	0	35	theme	nanoparticles	50:62	arg1	properties					23:32	Structural and optical properties	0:32	Structural and optical properties of Cu-doped ZnS nanoparticles	0:62	Structural and optical properties of Cu-doped ZnS nanoparticles formed in chitosan/sodium alginate multilayer films.
23818238	6	36	dep	ZnS	935:937	arg1	form					956:959	form	956:959	form	956:959	The ZnS:Cu nanoparticles form and are distributed uniformly in the composite multilayer films with a surface average height of 25 nm.
23818238	6	36	dep	ZnS	935:937	arg1	distributed					969:979	distributed	969:979	are distributed uniformly in the composite multilayer films with a surface average height of 25 nm	965:1062	The ZnS:Cu nanoparticles form and are distributed uniformly in the composite multilayer films with a surface average height of 25 nm.
23818238	6	37	with	films	1019:1023	arg1	height					1048:1053	a surface average height	1030:1053	a surface average height of 25 nm	1030:1062	The ZnS:Cu nanoparticles form and are distributed uniformly in the composite multilayer films with a surface average height of 25 nm.
23818238	2	38	theme	X-ray	371:375	arg1	spectroscopy					457:468	energy dispersive spectroscopy	439:468	energy dispersive spectroscopy (EDS)	439:474	The synthesized nanoparticles were characterized using an X-ray diffractometer (XRD), transmission electron microscopy (TEM), energy dispersive spectroscopy (EDS) and atomic force microscopy (AFM).
23818238	2	38	theme	X-ray	371:375	arg1	microscopy					421:430	transmission electron microscopy	399:430	transmission electron microscopy (TEM)	399:436	The synthesized nanoparticles were characterized using an X-ray diffractometer (XRD), transmission electron microscopy (TEM), energy dispersive spectroscopy (EDS) and atomic force microscopy (AFM).
23818238	2	38	theme	X-ray	371:375	arg1	XRD					393:395	XRD	393:395	XRD	393:395	The synthesized nanoparticles were characterized using an X-ray diffractometer (XRD), transmission electron microscopy (TEM), energy dispersive spectroscopy (EDS) and atomic force microscopy (AFM).
23818238	2	38	theme	X-ray	371:375	arg1	microscopy					493:502	atomic force microscopy	480:502	atomic force microscopy (AFM)	480:508	The synthesized nanoparticles were characterized using an X-ray diffractometer (XRD), transmission electron microscopy (TEM), energy dispersive spectroscopy (EDS) and atomic force microscopy (AFM).
23818238	2	38	theme	X-ray	371:375	arg1	diffractometer					377:390	an X-ray diffractometer	368:390	an X-ray diffractometer (XRD)	368:396	The synthesized nanoparticles were characterized using an X-ray diffractometer (XRD), transmission electron microscopy (TEM), energy dispersive spectroscopy (EDS) and atomic force microscopy (AFM).
23818238	0	39	theme	ZnS	46:48	arg1	nanoparticles					50:62	Cu-doped ZnS nanoparticles	37:62	Cu-doped ZnS nanoparticles	37:62	Structural and optical properties of Cu-doped ZnS nanoparticles formed in chitosan/sodium alginate multilayer films.
23818238	5	40	theme	%	915:915	arg1	level					924:928	a 1% doping level	912:928	a 1% doping level	912:928	The photoluminescence of the Cu-doped ZnS nanoparticles reached a maximum at a 1% doping level.
23818238	3	41	theme	size	609:612	arg1	of ~ 3 nm					614:622	an average crystal size of ~ 3 nm	590:622	an average crystal size of ~ 3 nm	590:622	The results showed that cubic zinc blende-structured ZnS:Cu nanoparticles with an average crystal size of ~ 3 nm were uniformly distributed.
23818238	4	42	theme	Cu	812:813	arg1	concentrations					819:832	Cu ion concentrations	812:832	Cu ion concentrations	812:832	UV-vis spectra indicate a large quantum size effect and the absorption edge for the ZnS:Cu nanoparticles slightly shifted to longer wavelengths with increasing Cu ion concentrations.
23818238	5	43	theme	doping	917:922	arg1	level					924:928	a 1% doping level	912:928	a 1% doping level	912:928	The photoluminescence of the Cu-doped ZnS nanoparticles reached a maximum at a 1% doping level.
23818238	3	44	theme	blende-structured	546:562	arg1	nanoparticles					571:583	cubic zinc blende-structured ZnS:Cu nanoparticles	535:583	cubic zinc blende-structured ZnS:Cu nanoparticles with an average crystal size of ~ 3 nm	535:622	The results showed that cubic zinc blende-structured ZnS:Cu nanoparticles with an average crystal size of ~ 3 nm were uniformly distributed.
23818238	5	45	theme	1	914:914	arg1	%					915:915	%	915:915	%	915:915	The photoluminescence of the Cu-doped ZnS nanoparticles reached a maximum at a 1% doping level.
23818238	4	46	theme	longer	777:782	arg1	wavelengths					784:794	longer wavelengths	777:794	longer wavelengths	777:794	UV-vis spectra indicate a large quantum size effect and the absorption edge for the ZnS:Cu nanoparticles slightly shifted to longer wavelengths with increasing Cu ion concentrations.
23818238	4	47	theme	quantum	684:690	arg1	effect					697:702	a large quantum size effect	676:702	a large quantum size effect	676:702	UV-vis spectra indicate a large quantum size effect and the absorption edge for the ZnS:Cu nanoparticles slightly shifted to longer wavelengths with increasing Cu ion concentrations.
23818238	6	48	theme	multilayer	1008:1017	arg1	films					1019:1023	the composite multilayer films	994:1023	the composite multilayer films with a surface average height of 25 nm	994:1062	The ZnS:Cu nanoparticles form and are distributed uniformly in the composite multilayer films with a surface average height of 25 nm.
23818238	3	49	theme	crystal	601:607	arg1	size					609:612	an average crystal size	590:612	an average crystal size of ~ 3 nm	590:622	The results showed that cubic zinc blende-structured ZnS:Cu nanoparticles with an average crystal size of ~ 3 nm were uniformly distributed.
23818238	4	50	theme	size	692:695	arg1	effect					697:702	a large quantum size effect	676:702	a large quantum size effect	676:702	UV-vis spectra indicate a large quantum size effect and the absorption edge for the ZnS:Cu nanoparticles slightly shifted to longer wavelengths with increasing Cu ion concentrations.
23818238	1	51	theme	ZnS	196:198	arg1	nanoparticles					203:215	ZnS:Cu nanoparticles	196:215	ZnS:Cu nanoparticles	196:215	Chitosan/alginate multilayers were fabricated using a spin-coating method, and ZnS:Cu nanoparticles were generated within the network of two natural polysaccharides, chitosan and sodium alginate.
23818238	6	52	theme	composite	998:1006	arg1	films					1019:1023	the composite multilayer films	994:1023	the composite multilayer films with a surface average height of 25 nm	994:1062	The ZnS:Cu nanoparticles form and are distributed uniformly in the composite multilayer films with a surface average height of 25 nm.
23818238	0	53	theme	optical	15:21	arg1	properties					23:32	Structural and optical properties	0:32	Structural and optical properties of Cu-doped ZnS nanoparticles	0:62	Structural and optical properties of Cu-doped ZnS nanoparticles formed in chitosan/sodium alginate multilayer films.
23818238	1	54	theme	spin-coating	171:182	arg1	method					184:189	a spin-coating method	169:189	a spin-coating method	169:189	Chitosan/alginate multilayers were fabricated using a spin-coating method, and ZnS:Cu nanoparticles were generated within the network of two natural polysaccharides, chitosan and sodium alginate.
26241490	1	0	theme	functional	274:283	arg1	properties					285:294	potential functional properties	264:294	potential functional properties	264:294	Many lactic acid bacteria synthesize extracellular polysaccharides (exopolysaccharides, EPSs) with a large variation in structure and potential functional properties.
26241490	0	1	theme	suebicus	92:99	arg1	strains					101:107	two Lactobacillus suebicus strains	74:107	two Lactobacillus suebicus strains isolated from cider	74:127	Production and partial characterization of exopolysaccharides produced by two Lactobacillus suebicus strains isolated from cider.
26241490	8	2	theme	conditions	1355:1364	arg1	influence					1278:1286	the influence	1274:1286	the influence of the sugar source (glucose, ribose, xylose, or arabinose) and pH conditions on growth and EPS production	1274:1393	We also examined the influence of the sugar source (glucose, ribose, xylose, or arabinose) and pH conditions on growth and EPS production.
26241490	5	3	theme	polymers	756:763	arg1	characterization					732:747	the preliminary characterization	716:747	the preliminary characterization of the polymers	716:763	In addition, the preliminary characterization of the polymers was undertaken.
26241490	7	4	theme	fractions	989:997	arg1	composition					966:976	The composition	962:976	The composition of the EPS fractions	962:997	The composition of the EPS fractions was analyzed by gas chromatography-mass spectrometry after acid hydrolysis, revealing that they contained glucose, galactose, N-acetylglucosamine and phosphate, although in different ratios, suggesting that a mixture of polysaccharides is being synthesized.
26241490	7	5	theme	different	1172:1180	arg1	ratios					1182:1187	different ratios	1172:1187	different ratios	1172:1187	The composition of the EPS fractions was analyzed by gas chromatography-mass spectrometry after acid hydrolysis, revealing that they contained glucose, galactose, N-acetylglucosamine and phosphate, although in different ratios, suggesting that a mixture of polysaccharides is being synthesized.
26241490	7	6	theme	gas	1015:1017	arg1	spectrometry					1039:1050	gas chromatography-mass spectrometry	1015:1050	gas chromatography-mass spectrometry	1015:1050	The composition of the EPS fractions was analyzed by gas chromatography-mass spectrometry after acid hydrolysis, revealing that they contained glucose, galactose, N-acetylglucosamine and phosphate, although in different ratios, suggesting that a mixture of polysaccharides is being synthesized.
26241490	3	7	theme	ropy	571:574	arg1	ciders					576:581	ropy ciders	571:581	ropy ciders	571:581	In this work, EPS production by two Lactobacillus suebicus strains, which were isolated from ropy ciders, was examined in a semidefined medium.
26241490	7	8	contain	contained	1095:1103	arg1	ratios					1182:1187	different ratios	1172:1187	different ratios	1172:1187	The composition of the EPS fractions was analyzed by gas chromatography-mass spectrometry after acid hydrolysis, revealing that they contained glucose, galactose, N-acetylglucosamine and phosphate, although in different ratios, suggesting that a mixture of polysaccharides is being synthesized.
26241490	7	8	contain	contained	1095:1103	arg1	they					1090:1093	they	1090:1093	they	1090:1093	The composition of the EPS fractions was analyzed by gas chromatography-mass spectrometry after acid hydrolysis, revealing that they contained glucose, galactose, N-acetylglucosamine and phosphate, although in different ratios, suggesting that a mixture of polysaccharides is being synthesized.
26241490	7	8	contain	contained	1095:1103	arg2	galactose					1114:1122	galactose	1114:1122	galactose	1114:1122	The composition of the EPS fractions was analyzed by gas chromatography-mass spectrometry after acid hydrolysis, revealing that they contained glucose, galactose, N-acetylglucosamine and phosphate, although in different ratios, suggesting that a mixture of polysaccharides is being synthesized.
26241490	7	8	contain	contained	1095:1103	arg2	phosphate					1149:1157	phosphate	1149:1157	phosphate	1149:1157	The composition of the EPS fractions was analyzed by gas chromatography-mass spectrometry after acid hydrolysis, revealing that they contained glucose, galactose, N-acetylglucosamine and phosphate, although in different ratios, suggesting that a mixture of polysaccharides is being synthesized.
26241490	7	8	contain	contained	1095:1103	arg2	N-acetylglucosamine					1125:1143	N-acetylglucosamine	1125:1143	N-acetylglucosamine	1125:1143	The composition of the EPS fractions was analyzed by gas chromatography-mass spectrometry after acid hydrolysis, revealing that they contained glucose, galactose, N-acetylglucosamine and phosphate, although in different ratios, suggesting that a mixture of polysaccharides is being synthesized.
26241490	7	8	contain	contained	1095:1103	arg2	they					1090:1093	they	1090:1093	they	1090:1093	The composition of the EPS fractions was analyzed by gas chromatography-mass spectrometry after acid hydrolysis, revealing that they contained glucose, galactose, N-acetylglucosamine and phosphate, although in different ratios, suggesting that a mixture of polysaccharides is being synthesized.
26241490	7	8	contain	contained	1095:1103	arg2	glucose					1105:1111	glucose	1105:1111	glucose	1105:1111	The composition of the EPS fractions was analyzed by gas chromatography-mass spectrometry after acid hydrolysis, revealing that they contained glucose, galactose, N-acetylglucosamine and phosphate, although in different ratios, suggesting that a mixture of polysaccharides is being synthesized.
26241490	3	9	theme	Lactobacillus	514:526	arg1	strains					537:543	two Lactobacillus suebicus strains	510:543	two Lactobacillus suebicus strains	510:543	In this work, EPS production by two Lactobacillus suebicus strains, which were isolated from ropy ciders, was examined in a semidefined medium.
26241490	8	10	theme	source	1301:1306	arg1	influence					1278:1286	the influence	1274:1286	the influence of the sugar source (glucose, ribose, xylose, or arabinose) and pH conditions on growth and EPS production	1274:1393	We also examined the influence of the sugar source (glucose, ribose, xylose, or arabinose) and pH conditions on growth and EPS production.
26241490	6	11	theme	low-molecular-weight	923:942	arg1	polymers					901:908	polymers	901:908	polymers of high- and low-molecular-weight in all fractions	901:959	Molecular masses were determined by size exclusion chromatography revealing the presence of two peaks, corresponding to polymers of high- and low-molecular-weight in all fractions.
26241490	4	12	theme	encoding	667:674	arg1	genes					676:680	priming glycosyltransferase encoding genes	639:680	priming glycosyltransferase encoding genes	639:680	The existence of priming glycosyltransferase encoding genes was detected by PCR.
26241490	2	13	theme	EPS	306:308	arg1	production					310:319	EPS production	306:319	EPS production	306:319	Although EPS production can produce detrimental effects in alcoholic beverages, these polymers play an important role in the rheological behavior and texture of fermented products.
26241490	2	14	theme	rheological	422:432	arg1	behavior					434:441	rheological behavior	422:441	rheological behavior	422:441	Although EPS production can produce detrimental effects in alcoholic beverages, these polymers play an important role in the rheological behavior and texture of fermented products.
26241490	6	15	from	polymers	901:908	arg1	fractions					951:959	all fractions	947:959	all fractions	947:959	Molecular masses were determined by size exclusion chromatography revealing the presence of two peaks, corresponding to polymers of high- and low-molecular-weight in all fractions.
26241490	6	16	theme	exclusion	822:830	arg1	chromatography					832:845	size exclusion chromatography	817:845	size exclusion chromatography revealing the presence of two peaks, corresponding to polymers of high- and low-molecular-weight in all fractions	817:959	Molecular masses were determined by size exclusion chromatography revealing the presence of two peaks, corresponding to polymers of high- and low-molecular-weight in all fractions.
26241490	2	17	theme	alcoholic	356:364	arg1	beverages					366:374	alcoholic beverages	356:374	alcoholic beverages	356:374	Although EPS production can produce detrimental effects in alcoholic beverages, these polymers play an important role in the rheological behavior and texture of fermented products.
26241490	8	18	from	influence	1278:1286	arg1	growth					1369:1374	growth	1369:1374	growth	1369:1374	We also examined the influence of the sugar source (glucose, ribose, xylose, or arabinose) and pH conditions on growth and EPS production.
26241490	8	18	from	influence	1278:1286	arg1	production					1384:1393	EPS production	1380:1393	EPS production	1380:1393	We also examined the influence of the sugar source (glucose, ribose, xylose, or arabinose) and pH conditions on growth and EPS production.
26241490	6	19	theme	size	817:820	arg1	chromatography					832:845	size exclusion chromatography	817:845	size exclusion chromatography revealing the presence of two peaks, corresponding to polymers of high- and low-molecular-weight in all fractions	817:959	Molecular masses were determined by size exclusion chromatography revealing the presence of two peaks, corresponding to polymers of high- and low-molecular-weight in all fractions.
26241490	6	20	from	high-	913:917	arg1	fractions					951:959	all fractions	947:959	all fractions	947:959	Molecular masses were determined by size exclusion chromatography revealing the presence of two peaks, corresponding to polymers of high- and low-molecular-weight in all fractions.
26241490	5	21	theme	preliminary	720:730	arg1	characterization					732:747	the preliminary characterization	716:747	the preliminary characterization of the polymers	716:763	In addition, the preliminary characterization of the polymers was undertaken.
26241490	8	22	dep	source	1301:1306	arg1	arabinose					1337:1345	arabinose	1337:1345	arabinose	1337:1345	We also examined the influence of the sugar source (glucose, ribose, xylose, or arabinose) and pH conditions on growth and EPS production.
26241490	8	22	dep	source	1301:1306	arg1	ribose					1318:1323	ribose	1318:1323	ribose	1318:1323	We also examined the influence of the sugar source (glucose, ribose, xylose, or arabinose) and pH conditions on growth and EPS production.
26241490	8	22	dep	source	1301:1306	arg1	glucose					1309:1315	glucose	1309:1315	glucose	1309:1315	We also examined the influence of the sugar source (glucose, ribose, xylose, or arabinose) and pH conditions on growth and EPS production.
26241490	8	22	dep	source	1301:1306	arg1	xylose					1326:1331	xylose	1326:1331	xylose	1326:1331	We also examined the influence of the sugar source (glucose, ribose, xylose, or arabinose) and pH conditions on growth and EPS production.
26241490	2	23	theme	fermented	458:466	arg1	products					468:475	fermented products	458:475	fermented products	458:475	Although EPS production can produce detrimental effects in alcoholic beverages, these polymers play an important role in the rheological behavior and texture of fermented products.
26241490	2	24	theme	products	468:475	arg1	texture					447:453	texture	447:453	texture	447:453	Although EPS production can produce detrimental effects in alcoholic beverages, these polymers play an important role in the rheological behavior and texture of fermented products.
26241490	2	24	theme	products	468:475	arg1	behavior					434:441	rheological behavior	422:441	rheological behavior	422:441	Although EPS production can produce detrimental effects in alcoholic beverages, these polymers play an important role in the rheological behavior and texture of fermented products.
26241490	6	25	theme	high-	913:917	arg1	polymers					901:908	polymers	901:908	polymers of high- and low-molecular-weight in all fractions	901:959	Molecular masses were determined by size exclusion chromatography revealing the presence of two peaks, corresponding to polymers of high- and low-molecular-weight in all fractions.
26241490	2	26	theme	detrimental	333:343	arg1	effects					345:351	detrimental effects	333:351	detrimental effects	333:351	Although EPS production can produce detrimental effects in alcoholic beverages, these polymers play an important role in the rheological behavior and texture of fermented products.
26241490	4	27	theme	glycosyltransferase	647:665	arg1	genes					676:680	priming glycosyltransferase encoding genes	639:680	priming glycosyltransferase encoding genes	639:680	The existence of priming glycosyltransferase encoding genes was detected by PCR.
26241490	0	28	theme	exopolysaccharides	43:60	arg1	Production					0:9	Production	0:9	Production	0:9	Production and partial characterization of exopolysaccharides produced by two Lactobacillus suebicus strains isolated from cider.
26241490	0	28	theme	exopolysaccharides	43:60	arg1	characterization					23:38	partial characterization	15:38	partial characterization	15:38	Production and partial characterization of exopolysaccharides produced by two Lactobacillus suebicus strains isolated from cider.
26241490	3	29	theme	suebicus	528:535	arg1	strains					537:543	two Lactobacillus suebicus strains	510:543	two Lactobacillus suebicus strains	510:543	In this work, EPS production by two Lactobacillus suebicus strains, which were isolated from ropy ciders, was examined in a semidefined medium.
26241490	3	30	attach	isolated	557:564	arg2	production					496:505	EPS production	492:505	EPS production	492:505	In this work, EPS production by two Lactobacillus suebicus strains, which were isolated from ropy ciders, was examined in a semidefined medium.
26241490	3	30	attach	isolated	557:564	arg1	ciders					576:581	ropy ciders	571:581	ropy ciders	571:581	In this work, EPS production by two Lactobacillus suebicus strains, which were isolated from ropy ciders, was examined in a semidefined medium.
26241490	1	31	theme	large	231:235	arg1	variation					237:245	a large variation	229:245	a large variation in structure and potential functional properties	229:294	Many lactic acid bacteria synthesize extracellular polysaccharides (exopolysaccharides, EPSs) with a large variation in structure and potential functional properties.
26241490	7	32	theme	polysaccharides	1219:1233	arg1	mixture					1208:1214	a mixture	1206:1214	a mixture of polysaccharides	1206:1233	The composition of the EPS fractions was analyzed by gas chromatography-mass spectrometry after acid hydrolysis, revealing that they contained glucose, galactose, N-acetylglucosamine and phosphate, although in different ratios, suggesting that a mixture of polysaccharides is being synthesized.
26241490	4	33	theme	genes	676:680	arg1	existence					626:634	The existence	622:634	The existence of priming glycosyltransferase encoding genes	622:680	The existence of priming glycosyltransferase encoding genes was detected by PCR.
26241490	1	34	dep	exopolysaccharides	198:215	arg1	EPSs					218:221	EPSs	218:221	EPSs	218:221	Many lactic acid bacteria synthesize extracellular polysaccharides (exopolysaccharides, EPSs) with a large variation in structure and potential functional properties.
26241490	1	35	from	variation	237:245	arg1	properties					285:294	potential functional properties	264:294	potential functional properties	264:294	Many lactic acid bacteria synthesize extracellular polysaccharides (exopolysaccharides, EPSs) with a large variation in structure and potential functional properties.
26241490	1	35	from	variation	237:245	arg1	structure					250:258	structure	250:258	structure	250:258	Many lactic acid bacteria synthesize extracellular polysaccharides (exopolysaccharides, EPSs) with a large variation in structure and potential functional properties.
26241490	1	36	theme	Many	130:133	arg1	bacteria					147:154	Many lactic acid bacteria	130:154	Many lactic acid bacteria	130:154	Many lactic acid bacteria synthesize extracellular polysaccharides (exopolysaccharides, EPSs) with a large variation in structure and potential functional properties.
26241490	3	37	theme	semidefined	602:612	arg1	medium					614:619	a semidefined medium	600:619	a semidefined medium	600:619	In this work, EPS production by two Lactobacillus suebicus strains, which were isolated from ropy ciders, was examined in a semidefined medium.
26241490	1	38	theme	lactic	135:140	arg1	bacteria					147:154	Many lactic acid bacteria	130:154	Many lactic acid bacteria	130:154	Many lactic acid bacteria synthesize extracellular polysaccharides (exopolysaccharides, EPSs) with a large variation in structure and potential functional properties.
26241490	7	39	theme	acid	1058:1061	arg1	hydrolysis					1063:1072	acid hydrolysis	1058:1072	acid hydrolysis	1058:1072	The composition of the EPS fractions was analyzed by gas chromatography-mass spectrometry after acid hydrolysis, revealing that they contained glucose, galactose, N-acetylglucosamine and phosphate, although in different ratios, suggesting that a mixture of polysaccharides is being synthesized.
26241490	7	40	theme	EPS	985:987	arg1	fractions					989:997	the EPS fractions	981:997	the EPS fractions	981:997	The composition of the EPS fractions was analyzed by gas chromatography-mass spectrometry after acid hydrolysis, revealing that they contained glucose, galactose, N-acetylglucosamine and phosphate, although in different ratios, suggesting that a mixture of polysaccharides is being synthesized.
26241490	6	41	theme	Molecular	781:789	arg1	masses					791:796	Molecular masses	781:796	Molecular masses	781:796	Molecular masses were determined by size exclusion chromatography revealing the presence of two peaks, corresponding to polymers of high- and low-molecular-weight in all fractions.
26241490	1	42	theme	acid	142:145	arg1	bacteria					147:154	Many lactic acid bacteria	130:154	Many lactic acid bacteria	130:154	Many lactic acid bacteria synthesize extracellular polysaccharides (exopolysaccharides, EPSs) with a large variation in structure and potential functional properties.
26241490	8	43	theme	EPS	1380:1382	arg1	production					1384:1393	EPS production	1380:1393	EPS production	1380:1393	We also examined the influence of the sugar source (glucose, ribose, xylose, or arabinose) and pH conditions on growth and EPS production.
26241490	6	44	theme	peaks	877:881	arg1	presence					861:868	the presence	857:868	the presence of two peaks, corresponding to polymers of high- and low-molecular-weight in all fractions	857:959	Molecular masses were determined by size exclusion chromatography revealing the presence of two peaks, corresponding to polymers of high- and low-molecular-weight in all fractions.
26241490	0	45	attach	isolated	109:116	arg1	cider					123:127	cider	123:127	cider	123:127	Production and partial characterization of exopolysaccharides produced by two Lactobacillus suebicus strains isolated from cider.
26241490	0	45	attach	isolated	109:116	arg2	strains					101:107	two Lactobacillus suebicus strains	74:107	two Lactobacillus suebicus strains isolated from cider	74:127	Production and partial characterization of exopolysaccharides produced by two Lactobacillus suebicus strains isolated from cider.
26241490	1	46	theme	extracellular	167:179	arg1	exopolysaccharides					198:215	exopolysaccharides	198:215	exopolysaccharides	198:215	Many lactic acid bacteria synthesize extracellular polysaccharides (exopolysaccharides, EPSs) with a large variation in structure and potential functional properties.
26241490	1	46	theme	extracellular	167:179	arg1	polysaccharides					181:195	extracellular polysaccharides	167:195	extracellular polysaccharides (exopolysaccharides, EPSs)	167:222	Many lactic acid bacteria synthesize extracellular polysaccharides (exopolysaccharides, EPSs) with a large variation in structure and potential functional properties.
26241490	0	47	theme	Lactobacillus	78:90	arg1	strains					101:107	two Lactobacillus suebicus strains	74:107	two Lactobacillus suebicus strains isolated from cider	74:127	Production and partial characterization of exopolysaccharides produced by two Lactobacillus suebicus strains isolated from cider.
26241490	2	48	dep	behavior	434:441	arg1	the					418:420	the	418:420	the	418:420	Although EPS production can produce detrimental effects in alcoholic beverages, these polymers play an important role in the rheological behavior and texture of fermented products.
26241490	3	49	theme	EPS	492:494	arg1	production					496:505	EPS production	492:505	EPS production	492:505	In this work, EPS production by two Lactobacillus suebicus strains, which were isolated from ropy ciders, was examined in a semidefined medium.
26241490	8	50	theme	pH	1352:1353	arg1	conditions					1355:1364	the sugar source (glucose, ribose, xylose, or arabinose) and pH conditions	1291:1364	conditions	1355:1364	We also examined the influence of the sugar source (glucose, ribose, xylose, or arabinose) and pH conditions on growth and EPS production.
26241490	8	51	theme	sugar	1295:1299	arg1	source					1301:1306	the sugar source (glucose, ribose, xylose, or arabinose) and pH conditions	1291:1364	source	1301:1306	We also examined the influence of the sugar source (glucose, ribose, xylose, or arabinose) and pH conditions on growth and EPS production.
26241490	4	52	theme	priming	639:645	arg1	genes					676:680	priming glycosyltransferase encoding genes	639:680	priming glycosyltransferase encoding genes	639:680	The existence of priming glycosyltransferase encoding genes was detected by PCR.
26241490	6	53	from	fractions	951:959	arg1	polymers					901:908	polymers	901:908	polymers of high- and low-molecular-weight in all fractions	901:959	Molecular masses were determined by size exclusion chromatography revealing the presence of two peaks, corresponding to polymers of high- and low-molecular-weight in all fractions.
26241490	6	53	from	fractions	951:959	arg1	high-					913:917	high-	913:917	high-	913:917	Molecular masses were determined by size exclusion chromatography revealing the presence of two peaks, corresponding to polymers of high- and low-molecular-weight in all fractions.
26241490	0	54	theme	partial	15:21	arg1	characterization					23:38	partial characterization	15:38	partial characterization	15:38	Production and partial characterization of exopolysaccharides produced by two Lactobacillus suebicus strains isolated from cider.
26241490	7	55	theme	chromatography-mass	1019:1037	arg1	spectrometry					1039:1050	gas chromatography-mass spectrometry	1015:1050	gas chromatography-mass spectrometry	1015:1050	The composition of the EPS fractions was analyzed by gas chromatography-mass spectrometry after acid hydrolysis, revealing that they contained glucose, galactose, N-acetylglucosamine and phosphate, although in different ratios, suggesting that a mixture of polysaccharides is being synthesized.
26241490	2	56	theme	important	400:408	arg1	role					410:413	an important role	397:413	an important role	397:413	Although EPS production can produce detrimental effects in alcoholic beverages, these polymers play an important role in the rheological behavior and texture of fermented products.
26241490	1	57	theme	potential	264:272	arg1	properties					285:294	potential functional properties	264:294	potential functional properties	264:294	Many lactic acid bacteria synthesize extracellular polysaccharides (exopolysaccharides, EPSs) with a large variation in structure and potential functional properties.
26337523	2	0	theme	PMP-labelled	892:903	arg1	sugars					905:910	PMP-labelled sugars	892:910	PMP-labelled sugars	892:910	However, the results of the present study re-performed for monosaccharide composition of this polysaccharide using, in addition to HPLC of PMP-labelled sugars, other separation methods, i.e. high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gas chromatography with flame ionising detection (GC-FID) and thin-layer chromatography (TLC), clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (38.6-49.4 mol %) and rhamnose (39.6-45 mol %), while mannose and galactose are present at a much lesser extent or in negligible amount.
26337523	1	1	theme	high-performance	412:427	arg1	analysis					458:465	high-performance liquid chromatography (HPLC) analysis	412:465	high-performance liquid chromatography (HPLC) analysis	412:465	Previously, we reported that the sulphated polysaccharides (SPS)-CF, a water-soluble polysaccharide isolated and purified from Korean green alga Maesaengi (Capsosiphon fulvescens, Chlorophyta), is a glucuronogalactomannan based mainly on the monosaccharide composition determined by high-performance liquid chromatography (HPLC) analysis after 1-phenyl-3-methyl-5-pyrazolone (PMP) labelling of sugars in the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF, which showed mannose (55.4 mol %), galactose (25.3 mol %) and glucuronic acid (16.3 mol %) as major sugars (Na et al., Int Immunopharmacol 10:364-370, 2010).
26337523	1	2	theme	Korean	256:261	arg1	Chlorophyta					309:319	Chlorophyta	309:319	Chlorophyta	309:319	Previously, we reported that the sulphated polysaccharides (SPS)-CF, a water-soluble polysaccharide isolated and purified from Korean green alga Maesaengi (Capsosiphon fulvescens, Chlorophyta), is a glucuronogalactomannan based mainly on the monosaccharide composition determined by high-performance liquid chromatography (HPLC) analysis after 1-phenyl-3-methyl-5-pyrazolone (PMP) labelling of sugars in the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF, which showed mannose (55.4 mol %), galactose (25.3 mol %) and glucuronic acid (16.3 mol %) as major sugars (Na et al., Int Immunopharmacol 10:364-370, 2010).
26337523	1	2	theme	Korean	256:261	arg1	Maesaengi					274:282	Korean green alga Maesaengi	256:282	Korean green alga Maesaengi (Capsosiphon fulvescens, Chlorophyta)	256:320	Previously, we reported that the sulphated polysaccharides (SPS)-CF, a water-soluble polysaccharide isolated and purified from Korean green alga Maesaengi (Capsosiphon fulvescens, Chlorophyta), is a glucuronogalactomannan based mainly on the monosaccharide composition determined by high-performance liquid chromatography (HPLC) analysis after 1-phenyl-3-methyl-5-pyrazolone (PMP) labelling of sugars in the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF, which showed mannose (55.4 mol %), galactose (25.3 mol %) and glucuronic acid (16.3 mol %) as major sugars (Na et al., Int Immunopharmacol 10:364-370, 2010).
26337523	2	3	theme	study	789:793	arg1	results					766:772	the results	762:772	the results of the present study re-performed for monosaccharide composition of this polysaccharide using	762:866	However, the results of the present study re-performed for monosaccharide composition of this polysaccharide using, in addition to HPLC of PMP-labelled sugars, other separation methods, i.e. high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gas chromatography with flame ionising detection (GC-FID) and thin-layer chromatography (TLC), clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (38.6-49.4 mol %) and rhamnose (39.6-45 mol %), while mannose and galactose are present at a much lesser extent or in negligible amount.
26337523	1	4	theme	alga	269:272	arg1	Chlorophyta					309:319	Chlorophyta	309:319	Chlorophyta	309:319	Previously, we reported that the sulphated polysaccharides (SPS)-CF, a water-soluble polysaccharide isolated and purified from Korean green alga Maesaengi (Capsosiphon fulvescens, Chlorophyta), is a glucuronogalactomannan based mainly on the monosaccharide composition determined by high-performance liquid chromatography (HPLC) analysis after 1-phenyl-3-methyl-5-pyrazolone (PMP) labelling of sugars in the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF, which showed mannose (55.4 mol %), galactose (25.3 mol %) and glucuronic acid (16.3 mol %) as major sugars (Na et al., Int Immunopharmacol 10:364-370, 2010).
26337523	1	4	theme	alga	269:272	arg1	Maesaengi					274:282	Korean green alga Maesaengi	256:282	Korean green alga Maesaengi (Capsosiphon fulvescens, Chlorophyta)	256:320	Previously, we reported that the sulphated polysaccharides (SPS)-CF, a water-soluble polysaccharide isolated and purified from Korean green alga Maesaengi (Capsosiphon fulvescens, Chlorophyta), is a glucuronogalactomannan based mainly on the monosaccharide composition determined by high-performance liquid chromatography (HPLC) analysis after 1-phenyl-3-methyl-5-pyrazolone (PMP) labelling of sugars in the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF, which showed mannose (55.4 mol %), galactose (25.3 mol %) and glucuronic acid (16.3 mol %) as major sugars (Na et al., Int Immunopharmacol 10:364-370, 2010).
26337523	5	5	attach	isolated	2166:2173	arg1	fulvescens					2200:2209	Korean Maesaengi C. fulvescens	2180:2209	Korean Maesaengi C. fulvescens	2180:2209	The results of this study demonstrated that the ulvan SPS-CF isolated from Korean Maesaengi C. fulvescens can be considered a potential anti-coagulant agent.
26337523	5	5	attach	isolated	2166:2173	arg2	SPS-CF					2159:2164	the ulvan SPS-CF	2149:2164	the ulvan SPS-CF isolated from Korean Maesaengi C. fulvescens	2149:2209	The results of this study demonstrated that the ulvan SPS-CF isolated from Korean Maesaengi C. fulvescens can be considered a potential anti-coagulant agent.
26337523	5	6	theme	study	2125:2129	arg1	results					2109:2115	The results	2105:2115	The results of this study	2105:2129	The results of this study demonstrated that the ulvan SPS-CF isolated from Korean Maesaengi C. fulvescens can be considered a potential anti-coagulant agent.
26337523	3	7	theme	units	1902:1906	arg1	O-2					1868:1870	O-2	1868:1870	O-2 of some L-rhamnose-3-sulphate units as the side chains	1868:1925	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	4	8	theme	partial	2017:2023	arg1	aPTT					2046:2049	aPTT	2046:2049	aPTT	2046:2049	The SPS-CF exhibited significant in vitro anti-coagulant activity by which the activated partial thromboplastin time (aPTT) and thrombin time (TT) were significantly prolonged.
26337523	4	8	theme	partial	2017:2023	arg1	time					2040:2043	the activated partial thromboplastin time	2003:2043	the activated partial thromboplastin time (aPTT)	2003:2050	The SPS-CF exhibited significant in vitro anti-coagulant activity by which the activated partial thromboplastin time (aPTT) and thrombin time (TT) were significantly prolonged.
26337523	4	8	theme	partial	2017:2023	arg1	prolonged					2094:2102	prolonged	2094:2102	prolonged	2094:2102	The SPS-CF exhibited significant in vitro anti-coagulant activity by which the activated partial thromboplastin time (aPTT) and thrombin time (TT) were significantly prolonged.
26337523	3	9	theme	polysaccharide	1657:1670	arg1	type					1642:1645	the sulphated glucuronorhamnoxylan (ulvan) type	1599:1645	the sulphated glucuronorhamnoxylan (ulvan) type	1599:1645	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	1	10	from	galactose	630:638	arg1	hydrolyzates					571:582	the acid (trifluoroacetic acid (TFA)) hydrolyzates	533:582	the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF	533:592	Previously, we reported that the sulphated polysaccharides (SPS)-CF, a water-soluble polysaccharide isolated and purified from Korean green alga Maesaengi (Capsosiphon fulvescens, Chlorophyta), is a glucuronogalactomannan based mainly on the monosaccharide composition determined by high-performance liquid chromatography (HPLC) analysis after 1-phenyl-3-methyl-5-pyrazolone (PMP) labelling of sugars in the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF, which showed mannose (55.4 mol %), galactose (25.3 mol %) and glucuronic acid (16.3 mol %) as major sugars (Na et al., Int Immunopharmacol 10:364-370, 2010).
26337523	2	11	located	present	1304:1310	arg1	extent					1329:1334	a much lesser extent	1315:1334	a much lesser extent	1315:1334	However, the results of the present study re-performed for monosaccharide composition of this polysaccharide using, in addition to HPLC of PMP-labelled sugars, other separation methods, i.e. high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gas chromatography with flame ionising detection (GC-FID) and thin-layer chromatography (TLC), clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (38.6-49.4 mol %) and rhamnose (39.6-45 mol %), while mannose and galactose are present at a much lesser extent or in negligible amount.
26337523	2	11	located	present	1304:1310	arg2	galactose					1290:1298	galactose	1290:1298	galactose	1290:1298	However, the results of the present study re-performed for monosaccharide composition of this polysaccharide using, in addition to HPLC of PMP-labelled sugars, other separation methods, i.e. high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gas chromatography with flame ionising detection (GC-FID) and thin-layer chromatography (TLC), clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (38.6-49.4 mol %) and rhamnose (39.6-45 mol %), while mannose and galactose are present at a much lesser extent or in negligible amount.
26337523	2	11	located	present	1304:1310	arg2	mannose					1278:1284	mannose	1278:1284	mannose	1278:1284	However, the results of the present study re-performed for monosaccharide composition of this polysaccharide using, in addition to HPLC of PMP-labelled sugars, other separation methods, i.e. high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gas chromatography with flame ionising detection (GC-FID) and thin-layer chromatography (TLC), clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (38.6-49.4 mol %) and rhamnose (39.6-45 mol %), while mannose and galactose are present at a much lesser extent or in negligible amount.
26337523	2	12	theme	neutral	1179:1185	arg1	xylose					1217:1222	xylose	1217:1222	xylose (38.6-49.4 mol %)	1217:1240	However, the results of the present study re-performed for monosaccharide composition of this polysaccharide using, in addition to HPLC of PMP-labelled sugars, other separation methods, i.e. high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gas chromatography with flame ionising detection (GC-FID) and thin-layer chromatography (TLC), clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (38.6-49.4 mol %) and rhamnose (39.6-45 mol %), while mannose and galactose are present at a much lesser extent or in negligible amount.
26337523	2	12	theme	neutral	1179:1185	arg1	monosaccharides					1187:1201	the most prominent neutral monosaccharides	1160:1201	the most prominent neutral monosaccharides of SPS-CF	1160:1211	However, the results of the present study re-performed for monosaccharide composition of this polysaccharide using, in addition to HPLC of PMP-labelled sugars, other separation methods, i.e. high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gas chromatography with flame ionising detection (GC-FID) and thin-layer chromatography (TLC), clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (38.6-49.4 mol %) and rhamnose (39.6-45 mol %), while mannose and galactose are present at a much lesser extent or in negligible amount.
26337523	3	13	theme	4-linked	1727:1734	arg1	L-rhamnose-3-sulphate					1736:1756	4-linked L-rhamnose-3-sulphate and D-xylose residues	1727:1778	L-rhamnose-3-sulphate	1736:1756	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	3	13	theme	4-linked	1727:1734	arg1	U3s					1791:1793	ulvobiose U3s	1781:1793	ulvobiose U3s	1781:1793	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	1	14	theme	water-soluble	200:212	arg1	polysaccharide					214:227	a water-soluble polysaccharide	198:227	a water-soluble polysaccharide isolated and purified from Korean green alga Maesaengi (Capsosiphon fulvescens, Chlorophyta)	198:320	Previously, we reported that the sulphated polysaccharides (SPS)-CF, a water-soluble polysaccharide isolated and purified from Korean green alga Maesaengi (Capsosiphon fulvescens, Chlorophyta), is a glucuronogalactomannan based mainly on the monosaccharide composition determined by high-performance liquid chromatography (HPLC) analysis after 1-phenyl-3-methyl-5-pyrazolone (PMP) labelling of sugars in the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF, which showed mannose (55.4 mol %), galactose (25.3 mol %) and glucuronic acid (16.3 mol %) as major sugars (Na et al., Int Immunopharmacol 10:364-370, 2010).
26337523	1	14	theme	water-soluble	200:212	arg1	-CF					193:195	the sulphated polysaccharides (SPS)-CF	158:195	the sulphated polysaccharides (SPS)-CF	158:195	Previously, we reported that the sulphated polysaccharides (SPS)-CF, a water-soluble polysaccharide isolated and purified from Korean green alga Maesaengi (Capsosiphon fulvescens, Chlorophyta), is a glucuronogalactomannan based mainly on the monosaccharide composition determined by high-performance liquid chromatography (HPLC) analysis after 1-phenyl-3-methyl-5-pyrazolone (PMP) labelling of sugars in the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF, which showed mannose (55.4 mol %), galactose (25.3 mol %) and glucuronic acid (16.3 mol %) as major sugars (Na et al., Int Immunopharmacol 10:364-370, 2010).
26337523	2	15	theme	mol	1235:1237	arg1	xylose					1217:1222	xylose	1217:1222	xylose (38.6-49.4 mol %)	1217:1240	However, the results of the present study re-performed for monosaccharide composition of this polysaccharide using, in addition to HPLC of PMP-labelled sugars, other separation methods, i.e. high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gas chromatography with flame ionising detection (GC-FID) and thin-layer chromatography (TLC), clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (38.6-49.4 mol %) and rhamnose (39.6-45 mol %), while mannose and galactose are present at a much lesser extent or in negligible amount.
26337523	2	15	theme	mol	1235:1237	arg1	%					1239:1239	38.6-49.4 mol %	1225:1239	38.6-49.4 mol %	1225:1239	However, the results of the present study re-performed for monosaccharide composition of this polysaccharide using, in addition to HPLC of PMP-labelled sugars, other separation methods, i.e. high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gas chromatography with flame ionising detection (GC-FID) and thin-layer chromatography (TLC), clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (38.6-49.4 mol %) and rhamnose (39.6-45 mol %), while mannose and galactose are present at a much lesser extent or in negligible amount.
26337523	2	16	theme	separation	919:928	arg1	methods					930:936	other separation methods	913:936	other separation methods	913:936	However, the results of the present study re-performed for monosaccharide composition of this polysaccharide using, in addition to HPLC of PMP-labelled sugars, other separation methods, i.e. high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gas chromatography with flame ionising detection (GC-FID) and thin-layer chromatography (TLC), clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (38.6-49.4 mol %) and rhamnose (39.6-45 mol %), while mannose and galactose are present at a much lesser extent or in negligible amount.
26337523	1	17	from	hydrolyzates	571:582	arg1	%					650:650	25.3 mol %	641:650	25.3 mol %	641:650	Previously, we reported that the sulphated polysaccharides (SPS)-CF, a water-soluble polysaccharide isolated and purified from Korean green alga Maesaengi (Capsosiphon fulvescens, Chlorophyta), is a glucuronogalactomannan based mainly on the monosaccharide composition determined by high-performance liquid chromatography (HPLC) analysis after 1-phenyl-3-methyl-5-pyrazolone (PMP) labelling of sugars in the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF, which showed mannose (55.4 mol %), galactose (25.3 mol %) and glucuronic acid (16.3 mol %) as major sugars (Na et al., Int Immunopharmacol 10:364-370, 2010).
26337523	1	17	from	hydrolyzates	571:582	arg1	acid					668:671	glucuronic acid	657:671	glucuronic acid	657:671	Previously, we reported that the sulphated polysaccharides (SPS)-CF, a water-soluble polysaccharide isolated and purified from Korean green alga Maesaengi (Capsosiphon fulvescens, Chlorophyta), is a glucuronogalactomannan based mainly on the monosaccharide composition determined by high-performance liquid chromatography (HPLC) analysis after 1-phenyl-3-methyl-5-pyrazolone (PMP) labelling of sugars in the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF, which showed mannose (55.4 mol %), galactose (25.3 mol %) and glucuronic acid (16.3 mol %) as major sugars (Na et al., Int Immunopharmacol 10:364-370, 2010).
26337523	1	17	from	hydrolyzates	571:582	arg1	galactose					630:638	galactose	630:638	galactose (25.3 mol %)	630:651	Previously, we reported that the sulphated polysaccharides (SPS)-CF, a water-soluble polysaccharide isolated and purified from Korean green alga Maesaengi (Capsosiphon fulvescens, Chlorophyta), is a glucuronogalactomannan based mainly on the monosaccharide composition determined by high-performance liquid chromatography (HPLC) analysis after 1-phenyl-3-methyl-5-pyrazolone (PMP) labelling of sugars in the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF, which showed mannose (55.4 mol %), galactose (25.3 mol %) and glucuronic acid (16.3 mol %) as major sugars (Na et al., Int Immunopharmacol 10:364-370, 2010).
26337523	1	17	from	hydrolyzates	571:582	arg1	labelling					510:518	1-phenyl-3-methyl-5-pyrazolone (PMP) labelling	473:518	1-phenyl-3-methyl-5-pyrazolone (PMP) labelling	473:518	Previously, we reported that the sulphated polysaccharides (SPS)-CF, a water-soluble polysaccharide isolated and purified from Korean green alga Maesaengi (Capsosiphon fulvescens, Chlorophyta), is a glucuronogalactomannan based mainly on the monosaccharide composition determined by high-performance liquid chromatography (HPLC) analysis after 1-phenyl-3-methyl-5-pyrazolone (PMP) labelling of sugars in the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF, which showed mannose (55.4 mol %), galactose (25.3 mol %) and glucuronic acid (16.3 mol %) as major sugars (Na et al., Int Immunopharmacol 10:364-370, 2010).
26337523	5	18	theme	Korean	2180:2185	arg1	fulvescens					2200:2209	Korean Maesaengi C. fulvescens	2180:2209	Korean Maesaengi C. fulvescens	2180:2209	The results of this study demonstrated that the ulvan SPS-CF isolated from Korean Maesaengi C. fulvescens can be considered a potential anti-coagulant agent.
26337523	1	19	theme	liquid	429:434	arg1	analysis					458:465	high-performance liquid chromatography (HPLC) analysis	412:465	high-performance liquid chromatography (HPLC) analysis	412:465	Previously, we reported that the sulphated polysaccharides (SPS)-CF, a water-soluble polysaccharide isolated and purified from Korean green alga Maesaengi (Capsosiphon fulvescens, Chlorophyta), is a glucuronogalactomannan based mainly on the monosaccharide composition determined by high-performance liquid chromatography (HPLC) analysis after 1-phenyl-3-methyl-5-pyrazolone (PMP) labelling of sugars in the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF, which showed mannose (55.4 mol %), galactose (25.3 mol %) and glucuronic acid (16.3 mol %) as major sugars (Na et al., Int Immunopharmacol 10:364-370, 2010).
26337523	4	20	theme	thrombin	2056:2063	arg1	TT					2071:2072	TT	2071:2072	TT	2071:2072	The SPS-CF exhibited significant in vitro anti-coagulant activity by which the activated partial thromboplastin time (aPTT) and thrombin time (TT) were significantly prolonged.
26337523	4	20	theme	thrombin	2056:2063	arg1	time					2065:2068	thrombin time	2056:2068	thrombin time (TT)	2056:2073	The SPS-CF exhibited significant in vitro anti-coagulant activity by which the activated partial thromboplastin time (aPTT) and thrombin time (TT) were significantly prolonged.
26337523	3	21	theme	magnetic	1422:1429	arg1	NMR					1442:1444	NMR	1442:1444	NMR	1442:1444	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	3	21	theme	magnetic	1422:1429	arg1	resonance					1431:1439	correlation nuclear magnetic resonance	1402:1439	correlation nuclear magnetic resonance (NMR)	1402:1445	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	3	21	theme	magnetic	1422:1429	arg1	analyses					1392:1399	These extensive monosaccharide analyses	1361:1399	These extensive monosaccharide analyses	1361:1399	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	5	22	theme	C.	2197:2198	arg1	fulvescens					2200:2209	Korean Maesaengi C. fulvescens	2180:2209	Korean Maesaengi C. fulvescens	2180:2209	The results of this study demonstrated that the ulvan SPS-CF isolated from Korean Maesaengi C. fulvescens can be considered a potential anti-coagulant agent.
26337523	2	23	theme	thin-layer	1101:1110	arg1	chromatography					1112:1125	thin-layer chromatography	1101:1125	thin-layer chromatography	1101:1125	However, the results of the present study re-performed for monosaccharide composition of this polysaccharide using, in addition to HPLC of PMP-labelled sugars, other separation methods, i.e. high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gas chromatography with flame ionising detection (GC-FID) and thin-layer chromatography (TLC), clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (38.6-49.4 mol %) and rhamnose (39.6-45 mol %), while mannose and galactose are present at a much lesser extent or in negligible amount.
26337523	3	24	theme	residues	1771:1778	arg1	sequence					1715:1722	alternating sequence	1703:1722	alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains	1703:1925	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	2	25	theme	high-performance	944:959	arg1	chromatography					976:989	high-performance anion-exchange chromatography	944:989	high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD)	944:1036	However, the results of the present study re-performed for monosaccharide composition of this polysaccharide using, in addition to HPLC of PMP-labelled sugars, other separation methods, i.e. high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gas chromatography with flame ionising detection (GC-FID) and thin-layer chromatography (TLC), clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (38.6-49.4 mol %) and rhamnose (39.6-45 mol %), while mannose and galactose are present at a much lesser extent or in negligible amount.
26337523	2	25	theme	high-performance	944:959	arg1	TLC					1128:1130	TLC	1128:1130	TLC	1128:1130	However, the results of the present study re-performed for monosaccharide composition of this polysaccharide using, in addition to HPLC of PMP-labelled sugars, other separation methods, i.e. high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gas chromatography with flame ionising detection (GC-FID) and thin-layer chromatography (TLC), clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (38.6-49.4 mol %) and rhamnose (39.6-45 mol %), while mannose and galactose are present at a much lesser extent or in negligible amount.
26337523	5	26	theme	ulvan	2153:2157	arg1	SPS-CF					2159:2164	the ulvan SPS-CF	2149:2164	the ulvan SPS-CF isolated from Korean Maesaengi C. fulvescens	2149:2209	The results of this study demonstrated that the ulvan SPS-CF isolated from Korean Maesaengi C. fulvescens can be considered a potential anti-coagulant agent.
26337523	0	27	theme	Polysaccharide	65:78	arg1	SPS-CF					80:85	the Sulphated Polysaccharide SPS-CF	51:85	the Sulphated Polysaccharide SPS-CF from a Green Alga	51:103	Structural Features and Anti-coagulant Activity of the Sulphated Polysaccharide SPS-CF from a Green Alga Capsosiphon fulvescens.
26337523	3	28	theme	extensive	1367:1375	arg1	spectrometry					1477:1488	electrospray ionization mass spectrometry	1448:1488	electrospray ionization mass spectrometry (ESI-MS)	1448:1497	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	3	28	theme	extensive	1367:1375	arg1	analyses					1392:1399	These extensive monosaccharide analyses	1361:1399	These extensive monosaccharide analyses	1361:1399	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	3	28	theme	extensive	1367:1375	arg1	resonance					1431:1439	correlation nuclear magnetic resonance	1402:1439	correlation nuclear magnetic resonance (NMR)	1402:1445	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	3	28	theme	extensive	1367:1375	arg1	spectrometry					1552:1563	matrix-assisted laser desorption/ionization mass spectrometry	1503:1563	matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS)	1503:1574	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	1	29	theme	mol	622:624	arg1	mannose					608:614	mannose	608:614	mannose (55.4 mol %)	608:627	Previously, we reported that the sulphated polysaccharides (SPS)-CF, a water-soluble polysaccharide isolated and purified from Korean green alga Maesaengi (Capsosiphon fulvescens, Chlorophyta), is a glucuronogalactomannan based mainly on the monosaccharide composition determined by high-performance liquid chromatography (HPLC) analysis after 1-phenyl-3-methyl-5-pyrazolone (PMP) labelling of sugars in the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF, which showed mannose (55.4 mol %), galactose (25.3 mol %) and glucuronic acid (16.3 mol %) as major sugars (Na et al., Int Immunopharmacol 10:364-370, 2010).
26337523	1	29	theme	mol	622:624	arg1	%					626:626	55.4 mol %	617:626	55.4 mol %	617:626	Previously, we reported that the sulphated polysaccharides (SPS)-CF, a water-soluble polysaccharide isolated and purified from Korean green alga Maesaengi (Capsosiphon fulvescens, Chlorophyta), is a glucuronogalactomannan based mainly on the monosaccharide composition determined by high-performance liquid chromatography (HPLC) analysis after 1-phenyl-3-methyl-5-pyrazolone (PMP) labelling of sugars in the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF, which showed mannose (55.4 mol %), galactose (25.3 mol %) and glucuronic acid (16.3 mol %) as major sugars (Na et al., Int Immunopharmacol 10:364-370, 2010).
26337523	2	30	from	present	1304:1310	arg1	amount					1353:1358	negligible amount	1342:1358	negligible amount	1342:1358	However, the results of the present study re-performed for monosaccharide composition of this polysaccharide using, in addition to HPLC of PMP-labelled sugars, other separation methods, i.e. high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gas chromatography with flame ionising detection (GC-FID) and thin-layer chromatography (TLC), clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (38.6-49.4 mol %) and rhamnose (39.6-45 mol %), while mannose and galactose are present at a much lesser extent or in negligible amount.
26337523	3	31	theme	matrix-assisted	1503:1517	arg1	analyses					1392:1399	These extensive monosaccharide analyses	1361:1399	These extensive monosaccharide analyses	1361:1399	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	3	31	theme	matrix-assisted	1503:1517	arg1	MALDI-MS					1566:1573	MALDI-MS	1566:1573	MALDI-MS	1566:1573	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	3	31	theme	matrix-assisted	1503:1517	arg1	spectrometry					1552:1563	matrix-assisted laser desorption/ionization mass spectrometry	1503:1563	matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS)	1503:1574	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	0	32	from	Alga	100:103	arg1	Features					11:18	Structural Features	0:18	Structural Features	0:18	Structural Features and Anti-coagulant Activity of the Sulphated Polysaccharide SPS-CF from a Green Alga Capsosiphon fulvescens.
26337523	0	32	from	Alga	100:103	arg1	Activity					39:46	Anti-coagulant Activity	24:46	Anti-coagulant Activity	24:46	Structural Features and Anti-coagulant Activity of the Sulphated Polysaccharide SPS-CF from a Green Alga Capsosiphon fulvescens.
26337523	0	32	from	Alga	100:103	arg1	SPS-CF					80:85	the Sulphated Polysaccharide SPS-CF	51:85	the Sulphated Polysaccharide SPS-CF from a Green Alga	51:103	Structural Features and Anti-coagulant Activity of the Sulphated Polysaccharide SPS-CF from a Green Alga Capsosiphon fulvescens.
26337523	1	33	theme	sulphated	162:170	arg1	SPS					189:191	SPS	189:191	SPS	189:191	Previously, we reported that the sulphated polysaccharides (SPS)-CF, a water-soluble polysaccharide isolated and purified from Korean green alga Maesaengi (Capsosiphon fulvescens, Chlorophyta), is a glucuronogalactomannan based mainly on the monosaccharide composition determined by high-performance liquid chromatography (HPLC) analysis after 1-phenyl-3-methyl-5-pyrazolone (PMP) labelling of sugars in the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF, which showed mannose (55.4 mol %), galactose (25.3 mol %) and glucuronic acid (16.3 mol %) as major sugars (Na et al., Int Immunopharmacol 10:364-370, 2010).
26337523	1	33	theme	sulphated	162:170	arg1	polysaccharides					172:186	the sulphated polysaccharides	158:186	the sulphated polysaccharides (SPS)-CF	158:195	Previously, we reported that the sulphated polysaccharides (SPS)-CF, a water-soluble polysaccharide isolated and purified from Korean green alga Maesaengi (Capsosiphon fulvescens, Chlorophyta), is a glucuronogalactomannan based mainly on the monosaccharide composition determined by high-performance liquid chromatography (HPLC) analysis after 1-phenyl-3-methyl-5-pyrazolone (PMP) labelling of sugars in the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF, which showed mannose (55.4 mol %), galactose (25.3 mol %) and glucuronic acid (16.3 mol %) as major sugars (Na et al., Int Immunopharmacol 10:364-370, 2010).
26337523	5	34	theme	potential	2231:2239	arg1	agent					2256:2260	a potential anti-coagulant agent	2229:2260	a potential anti-coagulant agent	2229:2260	The results of this study demonstrated that the ulvan SPS-CF isolated from Korean Maesaengi C. fulvescens can be considered a potential anti-coagulant agent.
26337523	2	35	theme	ionising	1069:1076	arg1	GC-FID					1089:1094	GC-FID	1089:1094	GC-FID	1089:1094	However, the results of the present study re-performed for monosaccharide composition of this polysaccharide using, in addition to HPLC of PMP-labelled sugars, other separation methods, i.e. high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gas chromatography with flame ionising detection (GC-FID) and thin-layer chromatography (TLC), clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (38.6-49.4 mol %) and rhamnose (39.6-45 mol %), while mannose and galactose are present at a much lesser extent or in negligible amount.
26337523	2	35	theme	ionising	1069:1076	arg1	detection					1078:1086	flame ionising detection	1063:1086	flame ionising detection (GC-FID)	1063:1095	However, the results of the present study re-performed for monosaccharide composition of this polysaccharide using, in addition to HPLC of PMP-labelled sugars, other separation methods, i.e. high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gas chromatography with flame ionising detection (GC-FID) and thin-layer chromatography (TLC), clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (38.6-49.4 mol %) and rhamnose (39.6-45 mol %), while mannose and galactose are present at a much lesser extent or in negligible amount.
26337523	0	36	theme	Green	94:98	arg1	Alga					100:103	a Green Alga	92:103	a Green Alga	92:103	Structural Features and Anti-coagulant Activity of the Sulphated Polysaccharide SPS-CF from a Green Alga Capsosiphon fulvescens.
26337523	3	37	theme	ulvobiose	1781:1789	arg1	L-rhamnose-3-sulphate					1736:1756	4-linked L-rhamnose-3-sulphate and D-xylose residues	1727:1778	L-rhamnose-3-sulphate	1736:1756	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	3	37	theme	ulvobiose	1781:1789	arg1	U3s					1791:1793	ulvobiose U3s	1781:1793	ulvobiose U3s	1781:1793	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	0	38	dep	Capsosiphon	105:115	arg1	Features					11:18	Structural Features	0:18	Structural Features	0:18	Structural Features and Anti-coagulant Activity of the Sulphated Polysaccharide SPS-CF from a Green Alga Capsosiphon fulvescens.
26337523	0	38	dep	Capsosiphon	105:115	arg1	Activity					39:46	Anti-coagulant Activity	24:46	Anti-coagulant Activity	24:46	Structural Features and Anti-coagulant Activity of the Sulphated Polysaccharide SPS-CF from a Green Alga Capsosiphon fulvescens.
26337523	0	38	dep	Capsosiphon	105:115	arg1	fulvescens					117:126	Capsosiphon fulvescens	105:126	Capsosiphon fulvescens	105:126	Structural Features and Anti-coagulant Activity of the Sulphated Polysaccharide SPS-CF from a Green Alga Capsosiphon fulvescens.
26337523	3	39	theme	mass	1472:1475	arg1	ESI-MS					1491:1496	ESI-MS	1491:1496	ESI-MS	1491:1496	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	3	39	theme	mass	1472:1475	arg1	spectrometry					1477:1488	electrospray ionization mass spectrometry	1448:1488	electrospray ionization mass spectrometry (ESI-MS)	1448:1497	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	3	39	theme	mass	1472:1475	arg1	analyses					1392:1399	These extensive monosaccharide analyses	1361:1399	These extensive monosaccharide analyses	1361:1399	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	4	40	dep	in	1961:1962	arg1	vitro					1964:1968	vitro	1964:1968	vitro	1964:1968	The SPS-CF exhibited significant in vitro anti-coagulant activity by which the activated partial thromboplastin time (aPTT) and thrombin time (TT) were significantly prolonged.
26337523	0	41	from	Activity	39:46	arg1	Alga					100:103	a Green Alga	92:103	a Green Alga	92:103	Structural Features and Anti-coagulant Activity of the Sulphated Polysaccharide SPS-CF from a Green Alga Capsosiphon fulvescens.
26337523	2	42	attach	present	1304:1310	arg1	extent					1329:1334	a much lesser extent	1315:1334	a much lesser extent	1315:1334	However, the results of the present study re-performed for monosaccharide composition of this polysaccharide using, in addition to HPLC of PMP-labelled sugars, other separation methods, i.e. high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gas chromatography with flame ionising detection (GC-FID) and thin-layer chromatography (TLC), clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (38.6-49.4 mol %) and rhamnose (39.6-45 mol %), while mannose and galactose are present at a much lesser extent or in negligible amount.
26337523	2	42	attach	present	1304:1310	arg1	amount					1353:1358	negligible amount	1342:1358	negligible amount	1342:1358	However, the results of the present study re-performed for monosaccharide composition of this polysaccharide using, in addition to HPLC of PMP-labelled sugars, other separation methods, i.e. high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gas chromatography with flame ionising detection (GC-FID) and thin-layer chromatography (TLC), clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (38.6-49.4 mol %) and rhamnose (39.6-45 mol %), while mannose and galactose are present at a much lesser extent or in negligible amount.
26337523	2	42	attach	present	1304:1310	arg2	galactose					1290:1298	galactose	1290:1298	galactose	1290:1298	However, the results of the present study re-performed for monosaccharide composition of this polysaccharide using, in addition to HPLC of PMP-labelled sugars, other separation methods, i.e. high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gas chromatography with flame ionising detection (GC-FID) and thin-layer chromatography (TLC), clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (38.6-49.4 mol %) and rhamnose (39.6-45 mol %), while mannose and galactose are present at a much lesser extent or in negligible amount.
26337523	2	42	attach	present	1304:1310	arg2	mannose					1278:1284	mannose	1278:1284	mannose	1278:1284	However, the results of the present study re-performed for monosaccharide composition of this polysaccharide using, in addition to HPLC of PMP-labelled sugars, other separation methods, i.e. high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gas chromatography with flame ionising detection (GC-FID) and thin-layer chromatography (TLC), clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (38.6-49.4 mol %) and rhamnose (39.6-45 mol %), while mannose and galactose are present at a much lesser extent or in negligible amount.
26337523	0	43	theme	Structural	0:9	arg1	Features					11:18	Structural Features	0:18	Structural Features	0:18	Structural Features and Anti-coagulant Activity of the Sulphated Polysaccharide SPS-CF from a Green Alga Capsosiphon fulvescens.
26337523	3	44	theme	correlation	1402:1412	arg1	NMR					1442:1444	NMR	1442:1444	NMR	1442:1444	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	3	44	theme	correlation	1402:1412	arg1	resonance					1431:1439	correlation nuclear magnetic resonance	1402:1439	correlation nuclear magnetic resonance (NMR)	1402:1445	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	3	44	theme	correlation	1402:1412	arg1	analyses					1392:1399	These extensive monosaccharide analyses	1361:1399	These extensive monosaccharide analyses	1361:1399	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	3	45	theme	monomeric	1805:1813	arg1	acid					1828:1831	monomeric D-glucuronic acid	1805:1831	monomeric D-glucuronic acid	1805:1831	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	1	46	theme	major	689:693	arg1	Na					703:704	Na	703:704	Na	703:704	Previously, we reported that the sulphated polysaccharides (SPS)-CF, a water-soluble polysaccharide isolated and purified from Korean green alga Maesaengi (Capsosiphon fulvescens, Chlorophyta), is a glucuronogalactomannan based mainly on the monosaccharide composition determined by high-performance liquid chromatography (HPLC) analysis after 1-phenyl-3-methyl-5-pyrazolone (PMP) labelling of sugars in the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF, which showed mannose (55.4 mol %), galactose (25.3 mol %) and glucuronic acid (16.3 mol %) as major sugars (Na et al., Int Immunopharmacol 10:364-370, 2010).
26337523	1	46	theme	major	689:693	arg1	sugars					695:700	major sugars	689:700	major sugars (Na et al., Int Immunopharmacol 10:364-370, 2010)	689:750	Previously, we reported that the sulphated polysaccharides (SPS)-CF, a water-soluble polysaccharide isolated and purified from Korean green alga Maesaengi (Capsosiphon fulvescens, Chlorophyta), is a glucuronogalactomannan based mainly on the monosaccharide composition determined by high-performance liquid chromatography (HPLC) analysis after 1-phenyl-3-methyl-5-pyrazolone (PMP) labelling of sugars in the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF, which showed mannose (55.4 mol %), galactose (25.3 mol %) and glucuronic acid (16.3 mol %) as major sugars (Na et al., Int Immunopharmacol 10:364-370, 2010).
26337523	2	47	theme	monosaccharide	812:825	arg1	composition					827:837	monosaccharide composition	812:837	monosaccharide composition of this polysaccharide using	812:866	However, the results of the present study re-performed for monosaccharide composition of this polysaccharide using, in addition to HPLC of PMP-labelled sugars, other separation methods, i.e. high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gas chromatography with flame ionising detection (GC-FID) and thin-layer chromatography (TLC), clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (38.6-49.4 mol %) and rhamnose (39.6-45 mol %), while mannose and galactose are present at a much lesser extent or in negligible amount.
26337523	1	48	from	sugars	523:528	arg1	hydrolyzates					571:582	the acid (trifluoroacetic acid (TFA)) hydrolyzates	533:582	the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF	533:592	Previously, we reported that the sulphated polysaccharides (SPS)-CF, a water-soluble polysaccharide isolated and purified from Korean green alga Maesaengi (Capsosiphon fulvescens, Chlorophyta), is a glucuronogalactomannan based mainly on the monosaccharide composition determined by high-performance liquid chromatography (HPLC) analysis after 1-phenyl-3-methyl-5-pyrazolone (PMP) labelling of sugars in the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF, which showed mannose (55.4 mol %), galactose (25.3 mol %) and glucuronic acid (16.3 mol %) as major sugars (Na et al., Int Immunopharmacol 10:364-370, 2010).
26337523	3	49	theme	desorption/ionization	1525:1545	arg1	analyses					1392:1399	These extensive monosaccharide analyses	1361:1399	These extensive monosaccharide analyses	1361:1399	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	3	49	theme	desorption/ionization	1525:1545	arg1	MALDI-MS					1566:1573	MALDI-MS	1566:1573	MALDI-MS	1566:1573	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	3	49	theme	desorption/ionization	1525:1545	arg1	spectrometry					1552:1563	matrix-assisted laser desorption/ionization mass spectrometry	1503:1563	matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS)	1503:1574	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	2	50	theme	amperometric	1003:1014	arg1	detection					1016:1024	pulsed amperometric detection	996:1024	pulsed amperometric detection (HPAEC-PAD)	996:1036	However, the results of the present study re-performed for monosaccharide composition of this polysaccharide using, in addition to HPLC of PMP-labelled sugars, other separation methods, i.e. high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gas chromatography with flame ionising detection (GC-FID) and thin-layer chromatography (TLC), clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (38.6-49.4 mol %) and rhamnose (39.6-45 mol %), while mannose and galactose are present at a much lesser extent or in negligible amount.
26337523	2	50	theme	amperometric	1003:1014	arg1	HPAEC-PAD					1027:1035	HPAEC-PAD	1027:1035	HPAEC-PAD	1027:1035	However, the results of the present study re-performed for monosaccharide composition of this polysaccharide using, in addition to HPLC of PMP-labelled sugars, other separation methods, i.e. high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gas chromatography with flame ionising detection (GC-FID) and thin-layer chromatography (TLC), clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (38.6-49.4 mol %) and rhamnose (39.6-45 mol %), while mannose and galactose are present at a much lesser extent or in negligible amount.
26337523	1	51	theme	sugars	523:528	arg1	%					650:650	25.3 mol %	641:650	25.3 mol %	641:650	Previously, we reported that the sulphated polysaccharides (SPS)-CF, a water-soluble polysaccharide isolated and purified from Korean green alga Maesaengi (Capsosiphon fulvescens, Chlorophyta), is a glucuronogalactomannan based mainly on the monosaccharide composition determined by high-performance liquid chromatography (HPLC) analysis after 1-phenyl-3-methyl-5-pyrazolone (PMP) labelling of sugars in the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF, which showed mannose (55.4 mol %), galactose (25.3 mol %) and glucuronic acid (16.3 mol %) as major sugars (Na et al., Int Immunopharmacol 10:364-370, 2010).
26337523	1	51	theme	sugars	523:528	arg1	acid					668:671	glucuronic acid	657:671	glucuronic acid	657:671	Previously, we reported that the sulphated polysaccharides (SPS)-CF, a water-soluble polysaccharide isolated and purified from Korean green alga Maesaengi (Capsosiphon fulvescens, Chlorophyta), is a glucuronogalactomannan based mainly on the monosaccharide composition determined by high-performance liquid chromatography (HPLC) analysis after 1-phenyl-3-methyl-5-pyrazolone (PMP) labelling of sugars in the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF, which showed mannose (55.4 mol %), galactose (25.3 mol %) and glucuronic acid (16.3 mol %) as major sugars (Na et al., Int Immunopharmacol 10:364-370, 2010).
26337523	1	51	theme	sugars	523:528	arg1	galactose					630:638	galactose	630:638	galactose (25.3 mol %)	630:651	Previously, we reported that the sulphated polysaccharides (SPS)-CF, a water-soluble polysaccharide isolated and purified from Korean green alga Maesaengi (Capsosiphon fulvescens, Chlorophyta), is a glucuronogalactomannan based mainly on the monosaccharide composition determined by high-performance liquid chromatography (HPLC) analysis after 1-phenyl-3-methyl-5-pyrazolone (PMP) labelling of sugars in the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF, which showed mannose (55.4 mol %), galactose (25.3 mol %) and glucuronic acid (16.3 mol %) as major sugars (Na et al., Int Immunopharmacol 10:364-370, 2010).
26337523	1	51	theme	sugars	523:528	arg1	labelling					510:518	1-phenyl-3-methyl-5-pyrazolone (PMP) labelling	473:518	1-phenyl-3-methyl-5-pyrazolone (PMP) labelling	473:518	Previously, we reported that the sulphated polysaccharides (SPS)-CF, a water-soluble polysaccharide isolated and purified from Korean green alga Maesaengi (Capsosiphon fulvescens, Chlorophyta), is a glucuronogalactomannan based mainly on the monosaccharide composition determined by high-performance liquid chromatography (HPLC) analysis after 1-phenyl-3-methyl-5-pyrazolone (PMP) labelling of sugars in the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF, which showed mannose (55.4 mol %), galactose (25.3 mol %) and glucuronic acid (16.3 mol %) as major sugars (Na et al., Int Immunopharmacol 10:364-370, 2010).
26337523	1	52	from	labelling	510:518	arg1	hydrolyzates					571:582	the acid (trifluoroacetic acid (TFA)) hydrolyzates	533:582	the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF	533:592	Previously, we reported that the sulphated polysaccharides (SPS)-CF, a water-soluble polysaccharide isolated and purified from Korean green alga Maesaengi (Capsosiphon fulvescens, Chlorophyta), is a glucuronogalactomannan based mainly on the monosaccharide composition determined by high-performance liquid chromatography (HPLC) analysis after 1-phenyl-3-methyl-5-pyrazolone (PMP) labelling of sugars in the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF, which showed mannose (55.4 mol %), galactose (25.3 mol %) and glucuronic acid (16.3 mol %) as major sugars (Na et al., Int Immunopharmacol 10:364-370, 2010).
26337523	2	53	theme	polysaccharide	847:860	arg1	composition					827:837	monosaccharide composition	812:837	monosaccharide composition of this polysaccharide using	812:866	However, the results of the present study re-performed for monosaccharide composition of this polysaccharide using, in addition to HPLC of PMP-labelled sugars, other separation methods, i.e. high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gas chromatography with flame ionising detection (GC-FID) and thin-layer chromatography (TLC), clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (38.6-49.4 mol %) and rhamnose (39.6-45 mol %), while mannose and galactose are present at a much lesser extent or in negligible amount.
26337523	2	54	theme	gas	1039:1041	arg1	chromatography					1043:1056	gas chromatography	1039:1056	gas chromatography with flame ionising detection (GC-FID)	1039:1095	However, the results of the present study re-performed for monosaccharide composition of this polysaccharide using, in addition to HPLC of PMP-labelled sugars, other separation methods, i.e. high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gas chromatography with flame ionising detection (GC-FID) and thin-layer chromatography (TLC), clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (38.6-49.4 mol %) and rhamnose (39.6-45 mol %), while mannose and galactose are present at a much lesser extent or in negligible amount.
26337523	3	55	theme	ulvan	1635:1639	arg1	type					1642:1645	the sulphated glucuronorhamnoxylan (ulvan) type	1599:1645	the sulphated glucuronorhamnoxylan (ulvan) type	1599:1645	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	1	56	theme	glucuronic	657:666	arg1	acid					668:671	glucuronic acid	657:671	glucuronic acid	657:671	Previously, we reported that the sulphated polysaccharides (SPS)-CF, a water-soluble polysaccharide isolated and purified from Korean green alga Maesaengi (Capsosiphon fulvescens, Chlorophyta), is a glucuronogalactomannan based mainly on the monosaccharide composition determined by high-performance liquid chromatography (HPLC) analysis after 1-phenyl-3-methyl-5-pyrazolone (PMP) labelling of sugars in the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF, which showed mannose (55.4 mol %), galactose (25.3 mol %) and glucuronic acid (16.3 mol %) as major sugars (Na et al., Int Immunopharmacol 10:364-370, 2010).
26337523	4	57	theme	significant	1949:1959	arg1	activity					1985:1992	significant in vitro anti-coagulant activity	1949:1992	significant in vitro anti-coagulant activity by which the activated partial thromboplastin time (aPTT) and thrombin time (TT) were significantly prolonged	1949:2102	The SPS-CF exhibited significant in vitro anti-coagulant activity by which the activated partial thromboplastin time (aPTT) and thrombin time (TT) were significantly prolonged.
26337523	3	58	theme	glucuronorhamnoxylan	1613:1632	arg1	type					1642:1645	the sulphated glucuronorhamnoxylan (ulvan) type	1599:1645	the sulphated glucuronorhamnoxylan (ulvan) type	1599:1645	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	1	59	dep	Na	703:704	arg1	al.					709:711	Na et al., Int Immunopharmacol 10:364-370, 2010	703:749	al.	709:711	Previously, we reported that the sulphated polysaccharides (SPS)-CF, a water-soluble polysaccharide isolated and purified from Korean green alga Maesaengi (Capsosiphon fulvescens, Chlorophyta), is a glucuronogalactomannan based mainly on the monosaccharide composition determined by high-performance liquid chromatography (HPLC) analysis after 1-phenyl-3-methyl-5-pyrazolone (PMP) labelling of sugars in the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF, which showed mannose (55.4 mol %), galactose (25.3 mol %) and glucuronic acid (16.3 mol %) as major sugars (Na et al., Int Immunopharmacol 10:364-370, 2010).
26337523	1	59	dep	Na	703:704	arg1	Immunopharmacol					718:732	Int Immunopharmacol 10:364-370	714:743	Int Immunopharmacol 10:364-370	714:743	Previously, we reported that the sulphated polysaccharides (SPS)-CF, a water-soluble polysaccharide isolated and purified from Korean green alga Maesaengi (Capsosiphon fulvescens, Chlorophyta), is a glucuronogalactomannan based mainly on the monosaccharide composition determined by high-performance liquid chromatography (HPLC) analysis after 1-phenyl-3-methyl-5-pyrazolone (PMP) labelling of sugars in the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF, which showed mannose (55.4 mol %), galactose (25.3 mol %) and glucuronic acid (16.3 mol %) as major sugars (Na et al., Int Immunopharmacol 10:364-370, 2010).
26337523	3	60	dep	spectrometry	1477:1488	arg1	measurements					1576:1587	measurements	1576:1587	measurements	1576:1587	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	1	61	dep	hydrolyzates	571:582	arg1	TFA					565:567	TFA	565:567	TFA	565:567	Previously, we reported that the sulphated polysaccharides (SPS)-CF, a water-soluble polysaccharide isolated and purified from Korean green alga Maesaengi (Capsosiphon fulvescens, Chlorophyta), is a glucuronogalactomannan based mainly on the monosaccharide composition determined by high-performance liquid chromatography (HPLC) analysis after 1-phenyl-3-methyl-5-pyrazolone (PMP) labelling of sugars in the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF, which showed mannose (55.4 mol %), galactose (25.3 mol %) and glucuronic acid (16.3 mol %) as major sugars (Na et al., Int Immunopharmacol 10:364-370, 2010).
26337523	1	61	dep	hydrolyzates	571:582	arg1	acid					559:562	trifluoroacetic acid	543:562	trifluoroacetic acid (TFA)	543:568	Previously, we reported that the sulphated polysaccharides (SPS)-CF, a water-soluble polysaccharide isolated and purified from Korean green alga Maesaengi (Capsosiphon fulvescens, Chlorophyta), is a glucuronogalactomannan based mainly on the monosaccharide composition determined by high-performance liquid chromatography (HPLC) analysis after 1-phenyl-3-methyl-5-pyrazolone (PMP) labelling of sugars in the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF, which showed mannose (55.4 mol %), galactose (25.3 mol %) and glucuronic acid (16.3 mol %) as major sugars (Na et al., Int Immunopharmacol 10:364-370, 2010).
26337523	1	62	dep	determined	398:407	arg1	%					683:683	16.3 mol %	674:683	16.3 mol %	674:683	Previously, we reported that the sulphated polysaccharides (SPS)-CF, a water-soluble polysaccharide isolated and purified from Korean green alga Maesaengi (Capsosiphon fulvescens, Chlorophyta), is a glucuronogalactomannan based mainly on the monosaccharide composition determined by high-performance liquid chromatography (HPLC) analysis after 1-phenyl-3-methyl-5-pyrazolone (PMP) labelling of sugars in the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF, which showed mannose (55.4 mol %), galactose (25.3 mol %) and glucuronic acid (16.3 mol %) as major sugars (Na et al., Int Immunopharmacol 10:364-370, 2010).
26337523	3	63	theme	SPS-CF	1650:1655	arg1	polysaccharide					1657:1670	SPS-CF polysaccharide	1650:1670	SPS-CF polysaccharide	1650:1670	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	2	64	theme	re-performed	795:806	arg1	study					789:793	the present study	777:793	the present study re-performed for monosaccharide composition of this polysaccharide using	777:866	However, the results of the present study re-performed for monosaccharide composition of this polysaccharide using, in addition to HPLC of PMP-labelled sugars, other separation methods, i.e. high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gas chromatography with flame ionising detection (GC-FID) and thin-layer chromatography (TLC), clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (38.6-49.4 mol %) and rhamnose (39.6-45 mol %), while mannose and galactose are present at a much lesser extent or in negligible amount.
26337523	1	65	theme	mol	679:681	arg1	%					683:683	16.3 mol %	674:683	16.3 mol %	674:683	Previously, we reported that the sulphated polysaccharides (SPS)-CF, a water-soluble polysaccharide isolated and purified from Korean green alga Maesaengi (Capsosiphon fulvescens, Chlorophyta), is a glucuronogalactomannan based mainly on the monosaccharide composition determined by high-performance liquid chromatography (HPLC) analysis after 1-phenyl-3-methyl-5-pyrazolone (PMP) labelling of sugars in the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF, which showed mannose (55.4 mol %), galactose (25.3 mol %) and glucuronic acid (16.3 mol %) as major sugars (Na et al., Int Immunopharmacol 10:364-370, 2010).
26337523	4	66	theme	activated	2007:2015	arg1	aPTT					2046:2049	aPTT	2046:2049	aPTT	2046:2049	The SPS-CF exhibited significant in vitro anti-coagulant activity by which the activated partial thromboplastin time (aPTT) and thrombin time (TT) were significantly prolonged.
26337523	4	66	theme	activated	2007:2015	arg1	time					2040:2043	the activated partial thromboplastin time	2003:2043	the activated partial thromboplastin time (aPTT)	2003:2050	The SPS-CF exhibited significant in vitro anti-coagulant activity by which the activated partial thromboplastin time (aPTT) and thrombin time (TT) were significantly prolonged.
26337523	4	66	theme	activated	2007:2015	arg1	prolonged					2094:2102	prolonged	2094:2102	prolonged	2094:2102	The SPS-CF exhibited significant in vitro anti-coagulant activity by which the activated partial thromboplastin time (aPTT) and thrombin time (TT) were significantly prolonged.
26337523	3	67	theme	D-glucuronic	1836:1847	arg1	acid-3-sulphate					1849:1863	D-glucuronic acid-3-sulphate	1836:1863	D-glucuronic acid-3-sulphate	1836:1863	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	1	68	theme	green	263:267	arg1	Chlorophyta					309:319	Chlorophyta	309:319	Chlorophyta	309:319	Previously, we reported that the sulphated polysaccharides (SPS)-CF, a water-soluble polysaccharide isolated and purified from Korean green alga Maesaengi (Capsosiphon fulvescens, Chlorophyta), is a glucuronogalactomannan based mainly on the monosaccharide composition determined by high-performance liquid chromatography (HPLC) analysis after 1-phenyl-3-methyl-5-pyrazolone (PMP) labelling of sugars in the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF, which showed mannose (55.4 mol %), galactose (25.3 mol %) and glucuronic acid (16.3 mol %) as major sugars (Na et al., Int Immunopharmacol 10:364-370, 2010).
26337523	1	68	theme	green	263:267	arg1	Maesaengi					274:282	Korean green alga Maesaengi	256:282	Korean green alga Maesaengi (Capsosiphon fulvescens, Chlorophyta)	256:320	Previously, we reported that the sulphated polysaccharides (SPS)-CF, a water-soluble polysaccharide isolated and purified from Korean green alga Maesaengi (Capsosiphon fulvescens, Chlorophyta), is a glucuronogalactomannan based mainly on the monosaccharide composition determined by high-performance liquid chromatography (HPLC) analysis after 1-phenyl-3-methyl-5-pyrazolone (PMP) labelling of sugars in the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF, which showed mannose (55.4 mol %), galactose (25.3 mol %) and glucuronic acid (16.3 mol %) as major sugars (Na et al., Int Immunopharmacol 10:364-370, 2010).
26337523	2	69	theme	present	781:787	arg1	study					789:793	the present study	777:793	the present study re-performed for monosaccharide composition of this polysaccharide using	777:866	However, the results of the present study re-performed for monosaccharide composition of this polysaccharide using, in addition to HPLC of PMP-labelled sugars, other separation methods, i.e. high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gas chromatography with flame ionising detection (GC-FID) and thin-layer chromatography (TLC), clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (38.6-49.4 mol %) and rhamnose (39.6-45 mol %), while mannose and galactose are present at a much lesser extent or in negligible amount.
26337523	3	70	theme	L-rhamnose-3-sulphate	1880:1900	arg1	units					1902:1906	some L-rhamnose-3-sulphate units	1875:1906	some L-rhamnose-3-sulphate units as the side chains	1875:1925	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	1	71	theme	1-phenyl-3-methyl-5-pyrazolone	473:502	arg1	labelling					510:518	1-phenyl-3-methyl-5-pyrazolone (PMP) labelling	473:518	1-phenyl-3-methyl-5-pyrazolone (PMP) labelling	473:518	Previously, we reported that the sulphated polysaccharides (SPS)-CF, a water-soluble polysaccharide isolated and purified from Korean green alga Maesaengi (Capsosiphon fulvescens, Chlorophyta), is a glucuronogalactomannan based mainly on the monosaccharide composition determined by high-performance liquid chromatography (HPLC) analysis after 1-phenyl-3-methyl-5-pyrazolone (PMP) labelling of sugars in the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF, which showed mannose (55.4 mol %), galactose (25.3 mol %) and glucuronic acid (16.3 mol %) as major sugars (Na et al., Int Immunopharmacol 10:364-370, 2010).
26337523	1	72	theme	polysaccharides	172:186	arg1	glucuronogalactomannan					328:349	a glucuronogalactomannan	326:349	a glucuronogalactomannan based mainly on the monosaccharide composition determined by high-performance liquid chromatography (HPLC) analysis after 1-phenyl-3-methyl-5-pyrazolone (PMP) labelling of sugars in the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF, which showed mannose (55.4 mol %), galactose (25.3 mol %) and glucuronic acid (16.3 mol %) as major sugars (Na et al., Int Immunopharmacol 10:364-370, 2010)	326:750	Previously, we reported that the sulphated polysaccharides (SPS)-CF, a water-soluble polysaccharide isolated and purified from Korean green alga Maesaengi (Capsosiphon fulvescens, Chlorophyta), is a glucuronogalactomannan based mainly on the monosaccharide composition determined by high-performance liquid chromatography (HPLC) analysis after 1-phenyl-3-methyl-5-pyrazolone (PMP) labelling of sugars in the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF, which showed mannose (55.4 mol %), galactose (25.3 mol %) and glucuronic acid (16.3 mol %) as major sugars (Na et al., Int Immunopharmacol 10:364-370, 2010).
26337523	1	72	theme	polysaccharides	172:186	arg1	polysaccharide					214:227	a water-soluble polysaccharide	198:227	a water-soluble polysaccharide isolated and purified from Korean green alga Maesaengi (Capsosiphon fulvescens, Chlorophyta)	198:320	Previously, we reported that the sulphated polysaccharides (SPS)-CF, a water-soluble polysaccharide isolated and purified from Korean green alga Maesaengi (Capsosiphon fulvescens, Chlorophyta), is a glucuronogalactomannan based mainly on the monosaccharide composition determined by high-performance liquid chromatography (HPLC) analysis after 1-phenyl-3-methyl-5-pyrazolone (PMP) labelling of sugars in the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF, which showed mannose (55.4 mol %), galactose (25.3 mol %) and glucuronic acid (16.3 mol %) as major sugars (Na et al., Int Immunopharmacol 10:364-370, 2010).
26337523	1	72	theme	polysaccharides	172:186	arg1	-CF					193:195	the sulphated polysaccharides (SPS)-CF	158:195	the sulphated polysaccharides (SPS)-CF	158:195	Previously, we reported that the sulphated polysaccharides (SPS)-CF, a water-soluble polysaccharide isolated and purified from Korean green alga Maesaengi (Capsosiphon fulvescens, Chlorophyta), is a glucuronogalactomannan based mainly on the monosaccharide composition determined by high-performance liquid chromatography (HPLC) analysis after 1-phenyl-3-methyl-5-pyrazolone (PMP) labelling of sugars in the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF, which showed mannose (55.4 mol %), galactose (25.3 mol %) and glucuronic acid (16.3 mol %) as major sugars (Na et al., Int Immunopharmacol 10:364-370, 2010).
26337523	2	73	theme	mol	1264:1266	arg1	%					1268:1268	39.6-45 mol %	1256:1268	39.6-45 mol %	1256:1268	However, the results of the present study re-performed for monosaccharide composition of this polysaccharide using, in addition to HPLC of PMP-labelled sugars, other separation methods, i.e. high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gas chromatography with flame ionising detection (GC-FID) and thin-layer chromatography (TLC), clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (38.6-49.4 mol %) and rhamnose (39.6-45 mol %), while mannose and galactose are present at a much lesser extent or in negligible amount.
26337523	2	73	theme	mol	1264:1266	arg1	rhamnose					1246:1253	rhamnose	1246:1253	rhamnose (39.6-45 mol %)	1246:1269	However, the results of the present study re-performed for monosaccharide composition of this polysaccharide using, in addition to HPLC of PMP-labelled sugars, other separation methods, i.e. high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gas chromatography with flame ionising detection (GC-FID) and thin-layer chromatography (TLC), clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (38.6-49.4 mol %) and rhamnose (39.6-45 mol %), while mannose and galactose are present at a much lesser extent or in negligible amount.
26337523	1	74	theme	PMP	505:507	arg1	labelling					510:518	1-phenyl-3-methyl-5-pyrazolone (PMP) labelling	473:518	1-phenyl-3-methyl-5-pyrazolone (PMP) labelling	473:518	Previously, we reported that the sulphated polysaccharides (SPS)-CF, a water-soluble polysaccharide isolated and purified from Korean green alga Maesaengi (Capsosiphon fulvescens, Chlorophyta), is a glucuronogalactomannan based mainly on the monosaccharide composition determined by high-performance liquid chromatography (HPLC) analysis after 1-phenyl-3-methyl-5-pyrazolone (PMP) labelling of sugars in the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF, which showed mannose (55.4 mol %), galactose (25.3 mol %) and glucuronic acid (16.3 mol %) as major sugars (Na et al., Int Immunopharmacol 10:364-370, 2010).
26337523	3	75	theme	side	1915:1918	arg1	chains					1920:1925	the side chains	1911:1925	the side chains	1911:1925	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	4	76	theme	thromboplastin	2025:2038	arg1	aPTT					2046:2049	aPTT	2046:2049	aPTT	2046:2049	The SPS-CF exhibited significant in vitro anti-coagulant activity by which the activated partial thromboplastin time (aPTT) and thrombin time (TT) were significantly prolonged.
26337523	4	76	theme	thromboplastin	2025:2038	arg1	time					2040:2043	the activated partial thromboplastin time	2003:2043	the activated partial thromboplastin time (aPTT)	2003:2050	The SPS-CF exhibited significant in vitro anti-coagulant activity by which the activated partial thromboplastin time (aPTT) and thrombin time (TT) were significantly prolonged.
26337523	4	76	theme	thromboplastin	2025:2038	arg1	prolonged					2094:2102	prolonged	2094:2102	prolonged	2094:2102	The SPS-CF exhibited significant in vitro anti-coagulant activity by which the activated partial thromboplastin time (aPTT) and thrombin time (TT) were significantly prolonged.
26337523	3	77	theme	alternating	1703:1713	arg1	sequence					1715:1722	alternating sequence	1703:1722	alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains	1703:1925	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	2	78	theme	SPS-CF	1206:1211	arg1	xylose					1217:1222	xylose	1217:1222	xylose (38.6-49.4 mol %)	1217:1240	However, the results of the present study re-performed for monosaccharide composition of this polysaccharide using, in addition to HPLC of PMP-labelled sugars, other separation methods, i.e. high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gas chromatography with flame ionising detection (GC-FID) and thin-layer chromatography (TLC), clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (38.6-49.4 mol %) and rhamnose (39.6-45 mol %), while mannose and galactose are present at a much lesser extent or in negligible amount.
26337523	2	78	theme	SPS-CF	1206:1211	arg1	monosaccharides					1187:1201	the most prominent neutral monosaccharides	1160:1201	the most prominent neutral monosaccharides of SPS-CF	1160:1211	However, the results of the present study re-performed for monosaccharide composition of this polysaccharide using, in addition to HPLC of PMP-labelled sugars, other separation methods, i.e. high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gas chromatography with flame ionising detection (GC-FID) and thin-layer chromatography (TLC), clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (38.6-49.4 mol %) and rhamnose (39.6-45 mol %), while mannose and galactose are present at a much lesser extent or in negligible amount.
26337523	3	79	theme	monosaccharide	1377:1390	arg1	spectrometry					1477:1488	electrospray ionization mass spectrometry	1448:1488	electrospray ionization mass spectrometry (ESI-MS)	1448:1497	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	3	79	theme	monosaccharide	1377:1390	arg1	analyses					1392:1399	These extensive monosaccharide analyses	1361:1399	These extensive monosaccharide analyses	1361:1399	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	3	79	theme	monosaccharide	1377:1390	arg1	resonance					1431:1439	correlation nuclear magnetic resonance	1402:1439	correlation nuclear magnetic resonance (NMR)	1402:1445	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	3	79	theme	monosaccharide	1377:1390	arg1	spectrometry					1552:1563	matrix-assisted laser desorption/ionization mass spectrometry	1503:1563	matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS)	1503:1574	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	2	80	with	chromatography	976:989	arg1	detection					1016:1024	pulsed amperometric detection	996:1024	pulsed amperometric detection (HPAEC-PAD)	996:1036	However, the results of the present study re-performed for monosaccharide composition of this polysaccharide using, in addition to HPLC of PMP-labelled sugars, other separation methods, i.e. high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gas chromatography with flame ionising detection (GC-FID) and thin-layer chromatography (TLC), clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (38.6-49.4 mol %) and rhamnose (39.6-45 mol %), while mannose and galactose are present at a much lesser extent or in negligible amount.
26337523	2	80	with	chromatography	976:989	arg1	GC-FID					1089:1094	GC-FID	1089:1094	GC-FID	1089:1094	However, the results of the present study re-performed for monosaccharide composition of this polysaccharide using, in addition to HPLC of PMP-labelled sugars, other separation methods, i.e. high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gas chromatography with flame ionising detection (GC-FID) and thin-layer chromatography (TLC), clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (38.6-49.4 mol %) and rhamnose (39.6-45 mol %), while mannose and galactose are present at a much lesser extent or in negligible amount.
26337523	2	80	with	chromatography	976:989	arg1	HPAEC-PAD					1027:1035	HPAEC-PAD	1027:1035	HPAEC-PAD	1027:1035	However, the results of the present study re-performed for monosaccharide composition of this polysaccharide using, in addition to HPLC of PMP-labelled sugars, other separation methods, i.e. high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gas chromatography with flame ionising detection (GC-FID) and thin-layer chromatography (TLC), clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (38.6-49.4 mol %) and rhamnose (39.6-45 mol %), while mannose and galactose are present at a much lesser extent or in negligible amount.
26337523	2	80	with	chromatography	976:989	arg1	detection					1078:1086	flame ionising detection	1063:1086	flame ionising detection (GC-FID)	1063:1095	However, the results of the present study re-performed for monosaccharide composition of this polysaccharide using, in addition to HPLC of PMP-labelled sugars, other separation methods, i.e. high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gas chromatography with flame ionising detection (GC-FID) and thin-layer chromatography (TLC), clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (38.6-49.4 mol %) and rhamnose (39.6-45 mol %), while mannose and galactose are present at a much lesser extent or in negligible amount.
26337523	1	81	theme	mol	646:648	arg1	%					650:650	25.3 mol %	641:650	25.3 mol %	641:650	Previously, we reported that the sulphated polysaccharides (SPS)-CF, a water-soluble polysaccharide isolated and purified from Korean green alga Maesaengi (Capsosiphon fulvescens, Chlorophyta), is a glucuronogalactomannan based mainly on the monosaccharide composition determined by high-performance liquid chromatography (HPLC) analysis after 1-phenyl-3-methyl-5-pyrazolone (PMP) labelling of sugars in the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF, which showed mannose (55.4 mol %), galactose (25.3 mol %) and glucuronic acid (16.3 mol %) as major sugars (Na et al., Int Immunopharmacol 10:364-370, 2010).
26337523	1	81	theme	mol	646:648	arg1	galactose					630:638	galactose	630:638	galactose (25.3 mol %)	630:651	Previously, we reported that the sulphated polysaccharides (SPS)-CF, a water-soluble polysaccharide isolated and purified from Korean green alga Maesaengi (Capsosiphon fulvescens, Chlorophyta), is a glucuronogalactomannan based mainly on the monosaccharide composition determined by high-performance liquid chromatography (HPLC) analysis after 1-phenyl-3-methyl-5-pyrazolone (PMP) labelling of sugars in the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF, which showed mannose (55.4 mol %), galactose (25.3 mol %) and glucuronic acid (16.3 mol %) as major sugars (Na et al., Int Immunopharmacol 10:364-370, 2010).
26337523	2	82	from	extent	1329:1334	arg1	present					1304:1310	present	1304:1310	present	1304:1310	However, the results of the present study re-performed for monosaccharide composition of this polysaccharide using, in addition to HPLC of PMP-labelled sugars, other separation methods, i.e. high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gas chromatography with flame ionising detection (GC-FID) and thin-layer chromatography (TLC), clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (38.6-49.4 mol %) and rhamnose (39.6-45 mol %), while mannose and galactose are present at a much lesser extent or in negligible amount.
26337523	1	83	dep	polysaccharide	214:227	arg1	purified					242:249	purified	242:249	purified from Korean green alga Maesaengi (Capsosiphon fulvescens, Chlorophyta)	242:320	Previously, we reported that the sulphated polysaccharides (SPS)-CF, a water-soluble polysaccharide isolated and purified from Korean green alga Maesaengi (Capsosiphon fulvescens, Chlorophyta), is a glucuronogalactomannan based mainly on the monosaccharide composition determined by high-performance liquid chromatography (HPLC) analysis after 1-phenyl-3-methyl-5-pyrazolone (PMP) labelling of sugars in the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF, which showed mannose (55.4 mol %), galactose (25.3 mol %) and glucuronic acid (16.3 mol %) as major sugars (Na et al., Int Immunopharmacol 10:364-370, 2010).
26337523	1	83	dep	polysaccharide	214:227	arg1	isolated					229:236	isolated	229:236	isolated	229:236	Previously, we reported that the sulphated polysaccharides (SPS)-CF, a water-soluble polysaccharide isolated and purified from Korean green alga Maesaengi (Capsosiphon fulvescens, Chlorophyta), is a glucuronogalactomannan based mainly on the monosaccharide composition determined by high-performance liquid chromatography (HPLC) analysis after 1-phenyl-3-methyl-5-pyrazolone (PMP) labelling of sugars in the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF, which showed mannose (55.4 mol %), galactose (25.3 mol %) and glucuronic acid (16.3 mol %) as major sugars (Na et al., Int Immunopharmacol 10:364-370, 2010).
26337523	2	84	theme	prominent	1169:1177	arg1	xylose					1217:1222	xylose	1217:1222	xylose (38.6-49.4 mol %)	1217:1240	However, the results of the present study re-performed for monosaccharide composition of this polysaccharide using, in addition to HPLC of PMP-labelled sugars, other separation methods, i.e. high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gas chromatography with flame ionising detection (GC-FID) and thin-layer chromatography (TLC), clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (38.6-49.4 mol %) and rhamnose (39.6-45 mol %), while mannose and galactose are present at a much lesser extent or in negligible amount.
26337523	2	84	theme	prominent	1169:1177	arg1	monosaccharides					1187:1201	the most prominent neutral monosaccharides	1160:1201	the most prominent neutral monosaccharides of SPS-CF	1160:1211	However, the results of the present study re-performed for monosaccharide composition of this polysaccharide using, in addition to HPLC of PMP-labelled sugars, other separation methods, i.e. high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gas chromatography with flame ionising detection (GC-FID) and thin-layer chromatography (TLC), clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (38.6-49.4 mol %) and rhamnose (39.6-45 mol %), while mannose and galactose are present at a much lesser extent or in negligible amount.
26337523	3	85	contain	carrying	1796:1803	arg2	acid					1828:1831	monomeric D-glucuronic acid	1805:1831	monomeric D-glucuronic acid	1805:1831	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	3	85	contain	carrying	1796:1803	arg1	L-rhamnose-3-sulphate					1736:1756	4-linked L-rhamnose-3-sulphate and D-xylose residues	1727:1778	L-rhamnose-3-sulphate	1736:1756	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	3	85	contain	carrying	1796:1803	arg1	U3s					1791:1793	ulvobiose U3s	1781:1793	ulvobiose U3s	1781:1793	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	3	85	contain	carrying	1796:1803	arg2	acid-3-sulphate					1849:1863	D-glucuronic acid-3-sulphate	1836:1863	D-glucuronic acid-3-sulphate	1836:1863	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	2	86	with	chromatography	1112:1125	arg1	detection					1016:1024	pulsed amperometric detection	996:1024	pulsed amperometric detection (HPAEC-PAD)	996:1036	However, the results of the present study re-performed for monosaccharide composition of this polysaccharide using, in addition to HPLC of PMP-labelled sugars, other separation methods, i.e. high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gas chromatography with flame ionising detection (GC-FID) and thin-layer chromatography (TLC), clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (38.6-49.4 mol %) and rhamnose (39.6-45 mol %), while mannose and galactose are present at a much lesser extent or in negligible amount.
26337523	2	86	with	chromatography	1112:1125	arg1	GC-FID					1089:1094	GC-FID	1089:1094	GC-FID	1089:1094	However, the results of the present study re-performed for monosaccharide composition of this polysaccharide using, in addition to HPLC of PMP-labelled sugars, other separation methods, i.e. high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gas chromatography with flame ionising detection (GC-FID) and thin-layer chromatography (TLC), clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (38.6-49.4 mol %) and rhamnose (39.6-45 mol %), while mannose and galactose are present at a much lesser extent or in negligible amount.
26337523	2	86	with	chromatography	1112:1125	arg1	HPAEC-PAD					1027:1035	HPAEC-PAD	1027:1035	HPAEC-PAD	1027:1035	However, the results of the present study re-performed for monosaccharide composition of this polysaccharide using, in addition to HPLC of PMP-labelled sugars, other separation methods, i.e. high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gas chromatography with flame ionising detection (GC-FID) and thin-layer chromatography (TLC), clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (38.6-49.4 mol %) and rhamnose (39.6-45 mol %), while mannose and galactose are present at a much lesser extent or in negligible amount.
26337523	2	86	with	chromatography	1112:1125	arg1	detection					1078:1086	flame ionising detection	1063:1086	flame ionising detection (GC-FID)	1063:1095	However, the results of the present study re-performed for monosaccharide composition of this polysaccharide using, in addition to HPLC of PMP-labelled sugars, other separation methods, i.e. high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gas chromatography with flame ionising detection (GC-FID) and thin-layer chromatography (TLC), clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (38.6-49.4 mol %) and rhamnose (39.6-45 mol %), while mannose and galactose are present at a much lesser extent or in negligible amount.
26337523	3	87	theme	L-rhamnose-3-sulphate	1736:1756	arg1	sequence					1715:1722	alternating sequence	1703:1722	alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains	1703:1925	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	3	88	link	4-linked	1727:1734	arg1	L-rhamnose-3-sulphate					1736:1756	4-linked L-rhamnose-3-sulphate and D-xylose residues	1727:1778	L-rhamnose-3-sulphate	1736:1756	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	3	88	link	4-linked	1727:1734	arg1	U3s					1791:1793	ulvobiose U3s	1781:1793	ulvobiose U3s	1781:1793	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	3	89	theme	nuclear	1414:1420	arg1	NMR					1442:1444	NMR	1442:1444	NMR	1442:1444	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	3	89	theme	nuclear	1414:1420	arg1	resonance					1431:1439	correlation nuclear magnetic resonance	1402:1439	correlation nuclear magnetic resonance (NMR)	1402:1445	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	3	89	theme	nuclear	1414:1420	arg1	analyses					1392:1399	These extensive monosaccharide analyses	1361:1399	These extensive monosaccharide analyses	1361:1399	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	5	90	theme	Maesaengi	2187:2195	arg1	fulvescens					2200:2209	Korean Maesaengi C. fulvescens	2180:2209	Korean Maesaengi C. fulvescens	2180:2209	The results of this study demonstrated that the ulvan SPS-CF isolated from Korean Maesaengi C. fulvescens can be considered a potential anti-coagulant agent.
26337523	2	91	from	amount	1353:1358	arg1	present					1304:1310	present	1304:1310	present	1304:1310	However, the results of the present study re-performed for monosaccharide composition of this polysaccharide using, in addition to HPLC of PMP-labelled sugars, other separation methods, i.e. high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gas chromatography with flame ionising detection (GC-FID) and thin-layer chromatography (TLC), clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (38.6-49.4 mol %) and rhamnose (39.6-45 mol %), while mannose and galactose are present at a much lesser extent or in negligible amount.
26337523	1	92	theme	chromatography	436:449	arg1	analysis					458:465	high-performance liquid chromatography (HPLC) analysis	412:465	high-performance liquid chromatography (HPLC) analysis	412:465	Previously, we reported that the sulphated polysaccharides (SPS)-CF, a water-soluble polysaccharide isolated and purified from Korean green alga Maesaengi (Capsosiphon fulvescens, Chlorophyta), is a glucuronogalactomannan based mainly on the monosaccharide composition determined by high-performance liquid chromatography (HPLC) analysis after 1-phenyl-3-methyl-5-pyrazolone (PMP) labelling of sugars in the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF, which showed mannose (55.4 mol %), galactose (25.3 mol %) and glucuronic acid (16.3 mol %) as major sugars (Na et al., Int Immunopharmacol 10:364-370, 2010).
26337523	2	93	theme	anion-exchange	961:974	arg1	chromatography					976:989	high-performance anion-exchange chromatography	944:989	high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD)	944:1036	However, the results of the present study re-performed for monosaccharide composition of this polysaccharide using, in addition to HPLC of PMP-labelled sugars, other separation methods, i.e. high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gas chromatography with flame ionising detection (GC-FID) and thin-layer chromatography (TLC), clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (38.6-49.4 mol %) and rhamnose (39.6-45 mol %), while mannose and galactose are present at a much lesser extent or in negligible amount.
26337523	2	93	theme	anion-exchange	961:974	arg1	TLC					1128:1130	TLC	1128:1130	TLC	1128:1130	However, the results of the present study re-performed for monosaccharide composition of this polysaccharide using, in addition to HPLC of PMP-labelled sugars, other separation methods, i.e. high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gas chromatography with flame ionising detection (GC-FID) and thin-layer chromatography (TLC), clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (38.6-49.4 mol %) and rhamnose (39.6-45 mol %), while mannose and galactose are present at a much lesser extent or in negligible amount.
26337523	0	94	theme	SPS-CF	80:85	arg1	Features					11:18	Structural Features	0:18	Structural Features	0:18	Structural Features and Anti-coagulant Activity of the Sulphated Polysaccharide SPS-CF from a Green Alga Capsosiphon fulvescens.
26337523	0	94	theme	SPS-CF	80:85	arg1	Activity					39:46	Anti-coagulant Activity	24:46	Anti-coagulant Activity	24:46	Structural Features and Anti-coagulant Activity of the Sulphated Polysaccharide SPS-CF from a Green Alga Capsosiphon fulvescens.
26337523	1	95	theme	HPLC	452:455	arg1	analysis					458:465	high-performance liquid chromatography (HPLC) analysis	412:465	high-performance liquid chromatography (HPLC) analysis	412:465	Previously, we reported that the sulphated polysaccharides (SPS)-CF, a water-soluble polysaccharide isolated and purified from Korean green alga Maesaengi (Capsosiphon fulvescens, Chlorophyta), is a glucuronogalactomannan based mainly on the monosaccharide composition determined by high-performance liquid chromatography (HPLC) analysis after 1-phenyl-3-methyl-5-pyrazolone (PMP) labelling of sugars in the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF, which showed mannose (55.4 mol %), galactose (25.3 mol %) and glucuronic acid (16.3 mol %) as major sugars (Na et al., Int Immunopharmacol 10:364-370, 2010).
26337523	2	96	dep	chromatography	976:989	arg1	i.e.					939:942	i.e.	939:942	i.e.	939:942	However, the results of the present study re-performed for monosaccharide composition of this polysaccharide using, in addition to HPLC of PMP-labelled sugars, other separation methods, i.e. high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gas chromatography with flame ionising detection (GC-FID) and thin-layer chromatography (TLC), clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (38.6-49.4 mol %) and rhamnose (39.6-45 mol %), while mannose and galactose are present at a much lesser extent or in negligible amount.
26337523	3	97	theme	D-xylose	1762:1769	arg1	residues					1771:1778	4-linked L-rhamnose-3-sulphate and D-xylose residues	1727:1778	residues	1771:1778	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	2	98	theme	other	913:917	arg1	methods					930:936	other separation methods	913:936	other separation methods	913:936	However, the results of the present study re-performed for monosaccharide composition of this polysaccharide using, in addition to HPLC of PMP-labelled sugars, other separation methods, i.e. high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gas chromatography with flame ionising detection (GC-FID) and thin-layer chromatography (TLC), clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (38.6-49.4 mol %) and rhamnose (39.6-45 mol %), while mannose and galactose are present at a much lesser extent or in negligible amount.
26337523	0	99	from	Features	11:18	arg1	Alga					100:103	a Green Alga	92:103	a Green Alga	92:103	Structural Features and Anti-coagulant Activity of the Sulphated Polysaccharide SPS-CF from a Green Alga Capsosiphon fulvescens.
26337523	2	100	theme	lesser	1322:1327	arg1	extent					1329:1334	a much lesser extent	1315:1334	a much lesser extent	1315:1334	However, the results of the present study re-performed for monosaccharide composition of this polysaccharide using, in addition to HPLC of PMP-labelled sugars, other separation methods, i.e. high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gas chromatography with flame ionising detection (GC-FID) and thin-layer chromatography (TLC), clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (38.6-49.4 mol %) and rhamnose (39.6-45 mol %), while mannose and galactose are present at a much lesser extent or in negligible amount.
26337523	4	101	theme	in	1961:1962	arg1	activity					1985:1992	significant in vitro anti-coagulant activity	1949:1992	significant in vitro anti-coagulant activity by which the activated partial thromboplastin time (aPTT) and thrombin time (TT) were significantly prolonged	1949:2102	The SPS-CF exhibited significant in vitro anti-coagulant activity by which the activated partial thromboplastin time (aPTT) and thrombin time (TT) were significantly prolonged.
26337523	3	102	theme	ionization	1461:1470	arg1	ESI-MS					1491:1496	ESI-MS	1491:1496	ESI-MS	1491:1496	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	3	102	theme	ionization	1461:1470	arg1	spectrometry					1477:1488	electrospray ionization mass spectrometry	1448:1488	electrospray ionization mass spectrometry (ESI-MS)	1448:1497	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	3	102	theme	ionization	1461:1470	arg1	analyses					1392:1399	These extensive monosaccharide analyses	1361:1399	These extensive monosaccharide analyses	1361:1399	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	5	103	theme	anti-coagulant	2241:2254	arg1	agent					2256:2260	a potential anti-coagulant agent	2229:2260	a potential anti-coagulant agent	2229:2260	The results of this study demonstrated that the ulvan SPS-CF isolated from Korean Maesaengi C. fulvescens can be considered a potential anti-coagulant agent.
26337523	2	104	theme	flame	1063:1067	arg1	GC-FID					1089:1094	GC-FID	1089:1094	GC-FID	1089:1094	However, the results of the present study re-performed for monosaccharide composition of this polysaccharide using, in addition to HPLC of PMP-labelled sugars, other separation methods, i.e. high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gas chromatography with flame ionising detection (GC-FID) and thin-layer chromatography (TLC), clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (38.6-49.4 mol %) and rhamnose (39.6-45 mol %), while mannose and galactose are present at a much lesser extent or in negligible amount.
26337523	2	104	theme	flame	1063:1067	arg1	detection					1078:1086	flame ionising detection	1063:1086	flame ionising detection (GC-FID)	1063:1095	However, the results of the present study re-performed for monosaccharide composition of this polysaccharide using, in addition to HPLC of PMP-labelled sugars, other separation methods, i.e. high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gas chromatography with flame ionising detection (GC-FID) and thin-layer chromatography (TLC), clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (38.6-49.4 mol %) and rhamnose (39.6-45 mol %), while mannose and galactose are present at a much lesser extent or in negligible amount.
26337523	2	105	theme	sugars	905:910	arg1	HPLC					884:887	HPLC	884:887	HPLC	884:887	However, the results of the present study re-performed for monosaccharide composition of this polysaccharide using, in addition to HPLC of PMP-labelled sugars, other separation methods, i.e. high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gas chromatography with flame ionising detection (GC-FID) and thin-layer chromatography (TLC), clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (38.6-49.4 mol %) and rhamnose (39.6-45 mol %), while mannose and galactose are present at a much lesser extent or in negligible amount.
26337523	1	106	theme	monosaccharide	371:384	arg1	composition					386:396	the monosaccharide composition	367:396	the monosaccharide composition determined by high-performance liquid chromatography (HPLC) analysis after 1-phenyl-3-methyl-5-pyrazolone (PMP) labelling of sugars in the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF, which showed mannose (55.4 mol %), galactose (25.3 mol %) and glucuronic acid (16.3 mol %) as major sugars (Na et al., Int Immunopharmacol 10:364-370, 2010)	367:750	Previously, we reported that the sulphated polysaccharides (SPS)-CF, a water-soluble polysaccharide isolated and purified from Korean green alga Maesaengi (Capsosiphon fulvescens, Chlorophyta), is a glucuronogalactomannan based mainly on the monosaccharide composition determined by high-performance liquid chromatography (HPLC) analysis after 1-phenyl-3-methyl-5-pyrazolone (PMP) labelling of sugars in the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF, which showed mannose (55.4 mol %), galactose (25.3 mol %) and glucuronic acid (16.3 mol %) as major sugars (Na et al., Int Immunopharmacol 10:364-370, 2010).
26337523	2	107	theme	negligible	1342:1351	arg1	amount					1353:1358	negligible amount	1342:1358	negligible amount	1342:1358	However, the results of the present study re-performed for monosaccharide composition of this polysaccharide using, in addition to HPLC of PMP-labelled sugars, other separation methods, i.e. high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gas chromatography with flame ionising detection (GC-FID) and thin-layer chromatography (TLC), clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (38.6-49.4 mol %) and rhamnose (39.6-45 mol %), while mannose and galactose are present at a much lesser extent or in negligible amount.
26337523	3	108	theme	electrospray	1448:1459	arg1	ESI-MS					1491:1496	ESI-MS	1491:1496	ESI-MS	1491:1496	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	3	108	theme	electrospray	1448:1459	arg1	spectrometry					1477:1488	electrospray ionization mass spectrometry	1448:1488	electrospray ionization mass spectrometry (ESI-MS)	1448:1497	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	3	108	theme	electrospray	1448:1459	arg1	analyses					1392:1399	These extensive monosaccharide analyses	1361:1399	These extensive monosaccharide analyses	1361:1399	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	0	109	theme	Anti-coagulant	24:37	arg1	Activity					39:46	Anti-coagulant Activity	24:46	Anti-coagulant Activity	24:46	Structural Features and Anti-coagulant Activity of the Sulphated Polysaccharide SPS-CF from a Green Alga Capsosiphon fulvescens.
26337523	3	110	theme	D-glucuronic	1815:1826	arg1	acid					1828:1831	monomeric D-glucuronic acid	1805:1831	monomeric D-glucuronic acid	1805:1831	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	1	111	theme	SPS-CF	587:592	arg1	hydrolyzates					571:582	the acid (trifluoroacetic acid (TFA)) hydrolyzates	533:582	the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF	533:592	Previously, we reported that the sulphated polysaccharides (SPS)-CF, a water-soluble polysaccharide isolated and purified from Korean green alga Maesaengi (Capsosiphon fulvescens, Chlorophyta), is a glucuronogalactomannan based mainly on the monosaccharide composition determined by high-performance liquid chromatography (HPLC) analysis after 1-phenyl-3-methyl-5-pyrazolone (PMP) labelling of sugars in the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF, which showed mannose (55.4 mol %), galactose (25.3 mol %) and glucuronic acid (16.3 mol %) as major sugars (Na et al., Int Immunopharmacol 10:364-370, 2010).
26337523	3	112	theme	laser	1519:1523	arg1	analyses					1392:1399	These extensive monosaccharide analyses	1361:1399	These extensive monosaccharide analyses	1361:1399	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	3	112	theme	laser	1519:1523	arg1	MALDI-MS					1566:1573	MALDI-MS	1566:1573	MALDI-MS	1566:1573	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	3	112	theme	laser	1519:1523	arg1	spectrometry					1552:1563	matrix-assisted laser desorption/ionization mass spectrometry	1503:1563	matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS)	1503:1574	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	0	113	theme	Sulphated	55:63	arg1	SPS-CF					80:85	the Sulphated Polysaccharide SPS-CF	51:85	the Sulphated Polysaccharide SPS-CF from a Green Alga	51:103	Structural Features and Anti-coagulant Activity of the Sulphated Polysaccharide SPS-CF from a Green Alga Capsosiphon fulvescens.
26337523	2	114	theme	using	862:866	arg1	polysaccharide					847:860	this polysaccharide using	842:866	this polysaccharide using	842:866	However, the results of the present study re-performed for monosaccharide composition of this polysaccharide using, in addition to HPLC of PMP-labelled sugars, other separation methods, i.e. high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gas chromatography with flame ionising detection (GC-FID) and thin-layer chromatography (TLC), clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (38.6-49.4 mol %) and rhamnose (39.6-45 mol %), while mannose and galactose are present at a much lesser extent or in negligible amount.
26337523	2	115	theme	pulsed	996:1001	arg1	detection					1016:1024	pulsed amperometric detection	996:1024	pulsed amperometric detection (HPAEC-PAD)	996:1036	However, the results of the present study re-performed for monosaccharide composition of this polysaccharide using, in addition to HPLC of PMP-labelled sugars, other separation methods, i.e. high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gas chromatography with flame ionising detection (GC-FID) and thin-layer chromatography (TLC), clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (38.6-49.4 mol %) and rhamnose (39.6-45 mol %), while mannose and galactose are present at a much lesser extent or in negligible amount.
26337523	2	115	theme	pulsed	996:1001	arg1	HPAEC-PAD					1027:1035	HPAEC-PAD	1027:1035	HPAEC-PAD	1027:1035	However, the results of the present study re-performed for monosaccharide composition of this polysaccharide using, in addition to HPLC of PMP-labelled sugars, other separation methods, i.e. high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gas chromatography with flame ionising detection (GC-FID) and thin-layer chromatography (TLC), clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (38.6-49.4 mol %) and rhamnose (39.6-45 mol %), while mannose and galactose are present at a much lesser extent or in negligible amount.
26337523	4	116	theme	anti-coagulant	1970:1983	arg1	activity					1985:1992	significant in vitro anti-coagulant activity	1949:1992	significant in vitro anti-coagulant activity by which the activated partial thromboplastin time (aPTT) and thrombin time (TT) were significantly prolonged	1949:2102	The SPS-CF exhibited significant in vitro anti-coagulant activity by which the activated partial thromboplastin time (aPTT) and thrombin time (TT) were significantly prolonged.
26337523	1	117	theme	Int	714:716	arg1	Immunopharmacol					718:732	Int Immunopharmacol 10:364-370	714:743	Int Immunopharmacol 10:364-370	714:743	Previously, we reported that the sulphated polysaccharides (SPS)-CF, a water-soluble polysaccharide isolated and purified from Korean green alga Maesaengi (Capsosiphon fulvescens, Chlorophyta), is a glucuronogalactomannan based mainly on the monosaccharide composition determined by high-performance liquid chromatography (HPLC) analysis after 1-phenyl-3-methyl-5-pyrazolone (PMP) labelling of sugars in the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF, which showed mannose (55.4 mol %), galactose (25.3 mol %) and glucuronic acid (16.3 mol %) as major sugars (Na et al., Int Immunopharmacol 10:364-370, 2010).
26337523	1	118	theme	acid	537:540	arg1	hydrolyzates					571:582	the acid (trifluoroacetic acid (TFA)) hydrolyzates	533:582	the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF	533:592	Previously, we reported that the sulphated polysaccharides (SPS)-CF, a water-soluble polysaccharide isolated and purified from Korean green alga Maesaengi (Capsosiphon fulvescens, Chlorophyta), is a glucuronogalactomannan based mainly on the monosaccharide composition determined by high-performance liquid chromatography (HPLC) analysis after 1-phenyl-3-methyl-5-pyrazolone (PMP) labelling of sugars in the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF, which showed mannose (55.4 mol %), galactose (25.3 mol %) and glucuronic acid (16.3 mol %) as major sugars (Na et al., Int Immunopharmacol 10:364-370, 2010).
26337523	3	119	theme	sulphated	1603:1611	arg1	type					1642:1645	the sulphated glucuronorhamnoxylan (ulvan) type	1599:1645	the sulphated glucuronorhamnoxylan (ulvan) type	1599:1645	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	2	120	with	chromatography	1043:1056	arg1	detection					1016:1024	pulsed amperometric detection	996:1024	pulsed amperometric detection (HPAEC-PAD)	996:1036	However, the results of the present study re-performed for monosaccharide composition of this polysaccharide using, in addition to HPLC of PMP-labelled sugars, other separation methods, i.e. high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gas chromatography with flame ionising detection (GC-FID) and thin-layer chromatography (TLC), clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (38.6-49.4 mol %) and rhamnose (39.6-45 mol %), while mannose and galactose are present at a much lesser extent or in negligible amount.
26337523	2	120	with	chromatography	1043:1056	arg1	GC-FID					1089:1094	GC-FID	1089:1094	GC-FID	1089:1094	However, the results of the present study re-performed for monosaccharide composition of this polysaccharide using, in addition to HPLC of PMP-labelled sugars, other separation methods, i.e. high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gas chromatography with flame ionising detection (GC-FID) and thin-layer chromatography (TLC), clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (38.6-49.4 mol %) and rhamnose (39.6-45 mol %), while mannose and galactose are present at a much lesser extent or in negligible amount.
26337523	2	120	with	chromatography	1043:1056	arg1	HPAEC-PAD					1027:1035	HPAEC-PAD	1027:1035	HPAEC-PAD	1027:1035	However, the results of the present study re-performed for monosaccharide composition of this polysaccharide using, in addition to HPLC of PMP-labelled sugars, other separation methods, i.e. high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gas chromatography with flame ionising detection (GC-FID) and thin-layer chromatography (TLC), clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (38.6-49.4 mol %) and rhamnose (39.6-45 mol %), while mannose and galactose are present at a much lesser extent or in negligible amount.
26337523	2	120	with	chromatography	1043:1056	arg1	detection					1078:1086	flame ionising detection	1063:1086	flame ionising detection (GC-FID)	1063:1095	However, the results of the present study re-performed for monosaccharide composition of this polysaccharide using, in addition to HPLC of PMP-labelled sugars, other separation methods, i.e. high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gas chromatography with flame ionising detection (GC-FID) and thin-layer chromatography (TLC), clearly demonstrated that the most prominent neutral monosaccharides of SPS-CF are xylose (38.6-49.4 mol %) and rhamnose (39.6-45 mol %), while mannose and galactose are present at a much lesser extent or in negligible amount.
26337523	1	121	from	acid	668:671	arg1	hydrolyzates					571:582	the acid (trifluoroacetic acid (TFA)) hydrolyzates	533:582	the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF	533:592	Previously, we reported that the sulphated polysaccharides (SPS)-CF, a water-soluble polysaccharide isolated and purified from Korean green alga Maesaengi (Capsosiphon fulvescens, Chlorophyta), is a glucuronogalactomannan based mainly on the monosaccharide composition determined by high-performance liquid chromatography (HPLC) analysis after 1-phenyl-3-methyl-5-pyrazolone (PMP) labelling of sugars in the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF, which showed mannose (55.4 mol %), galactose (25.3 mol %) and glucuronic acid (16.3 mol %) as major sugars (Na et al., Int Immunopharmacol 10:364-370, 2010).
26337523	3	122	theme	mass	1547:1550	arg1	analyses					1392:1399	These extensive monosaccharide analyses	1361:1399	These extensive monosaccharide analyses	1361:1399	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	3	122	theme	mass	1547:1550	arg1	MALDI-MS					1566:1573	MALDI-MS	1566:1573	MALDI-MS	1566:1573	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	3	122	theme	mass	1547:1550	arg1	spectrometry					1552:1563	matrix-assisted laser desorption/ionization mass spectrometry	1503:1563	matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS)	1503:1574	These extensive monosaccharide analyses, correlation nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) measurements confirmed the sulphated glucuronorhamnoxylan (ulvan) type of SPS-CF polysaccharide, whose backbone is composed of alternating sequence of 4-linked L-rhamnose-3-sulphate and D-xylose residues (ulvobiose U3s) carrying monomeric D-glucuronic acid or D-glucuronic acid-3-sulphate on O-2 of some L-rhamnose-3-sulphate units as the side chains.
26337523	1	123	theme	trifluoroacetic	543:557	arg1	TFA					565:567	TFA	565:567	TFA	565:567	Previously, we reported that the sulphated polysaccharides (SPS)-CF, a water-soluble polysaccharide isolated and purified from Korean green alga Maesaengi (Capsosiphon fulvescens, Chlorophyta), is a glucuronogalactomannan based mainly on the monosaccharide composition determined by high-performance liquid chromatography (HPLC) analysis after 1-phenyl-3-methyl-5-pyrazolone (PMP) labelling of sugars in the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF, which showed mannose (55.4 mol %), galactose (25.3 mol %) and glucuronic acid (16.3 mol %) as major sugars (Na et al., Int Immunopharmacol 10:364-370, 2010).
26337523	1	123	theme	trifluoroacetic	543:557	arg1	acid					559:562	trifluoroacetic acid	543:562	trifluoroacetic acid (TFA)	543:568	Previously, we reported that the sulphated polysaccharides (SPS)-CF, a water-soluble polysaccharide isolated and purified from Korean green alga Maesaengi (Capsosiphon fulvescens, Chlorophyta), is a glucuronogalactomannan based mainly on the monosaccharide composition determined by high-performance liquid chromatography (HPLC) analysis after 1-phenyl-3-methyl-5-pyrazolone (PMP) labelling of sugars in the acid (trifluoroacetic acid (TFA)) hydrolyzates of SPS-CF, which showed mannose (55.4 mol %), galactose (25.3 mol %) and glucuronic acid (16.3 mol %) as major sugars (Na et al., Int Immunopharmacol 10:364-370, 2010).
27161557	0	0	theme	antioxidant	105:115	arg1	activity					117:124	antioxidant activity	105:124	antioxidant activity	105:124	Ultrasonically extracted β-d-glucan from artificially cultivated mushroom, characteristic properties and antioxidant activity.
27161557	4	1	theme	activity	961:968	arg1	assays					935:940	the antioxidant in vitro assays	910:940	the antioxidant in vitro assays of DPPH scavenging activity and ferric reducing power (FRAP)	910:1001	The physical characteristics by HPAEC-PAD, HPGPC and FTIR, as well as the antioxidant in vitro assays of DPPH scavenging activity and ferric reducing power (FRAP) indicated that (1-3; 1-6)-β-d-glucans of Malaysian mushroom have better antioxidant activity, higher molecular weight and optimal degree of branching when extracted by US in comparison with conventional methods.
27161557	4	1	theme	activity	961:968	arg1	characteristics					853:867	The physical characteristics	840:867	The physical characteristics by HPAEC-PAD, HPGPC and FTIR	840:896	The physical characteristics by HPAEC-PAD, HPGPC and FTIR, as well as the antioxidant in vitro assays of DPPH scavenging activity and ferric reducing power (FRAP) indicated that (1-3; 1-6)-β-d-glucans of Malaysian mushroom have better antioxidant activity, higher molecular weight and optimal degree of branching when extracted by US in comparison with conventional methods.
27161557	3	2	dep	-β-d-glucans	635:646	arg1	yield					748:752	the total yield	738:752	the total yield of polysaccharides that could be obtained from the artificially cultivated Ganoderma	738:837	Therefore three extraction methods i.e. hot water extraction (HWE), soxhlet extraction (SE) and ultrasound assisted extraction (US) were employed to study the total content of (1-3; 1-6)-β-d-glucans, degree of branching, structural characteristics, monosaccharides composition, as well as the total yield of polysaccharides that could be obtained from the artificially cultivated Ganoderma.
27161557	3	2	dep	-β-d-glucans	635:646	arg1	composition					714:724	composition	714:724	composition	714:724	Therefore three extraction methods i.e. hot water extraction (HWE), soxhlet extraction (SE) and ultrasound assisted extraction (US) were employed to study the total content of (1-3; 1-6)-β-d-glucans, degree of branching, structural characteristics, monosaccharides composition, as well as the total yield of polysaccharides that could be obtained from the artificially cultivated Ganoderma.
27161557	3	2	dep	-β-d-glucans	635:646	arg1	-β-d-glucans					635:646	(1-3; 1-6)-β-d-glucans	625:646	(1-3; 1-6)-β-d-glucans	625:646	Therefore three extraction methods i.e. hot water extraction (HWE), soxhlet extraction (SE) and ultrasound assisted extraction (US) were employed to study the total content of (1-3; 1-6)-β-d-glucans, degree of branching, structural characteristics, monosaccharides composition, as well as the total yield of polysaccharides that could be obtained from the artificially cultivated Ganoderma.
27161557	4	3	theme	scavenging	950:959	arg1	activity					961:968	DPPH scavenging activity	945:968	DPPH scavenging activity	945:968	The physical characteristics by HPAEC-PAD, HPGPC and FTIR, as well as the antioxidant in vitro assays of DPPH scavenging activity and ferric reducing power (FRAP) indicated that (1-3; 1-6)-β-d-glucans of Malaysian mushroom have better antioxidant activity, higher molecular weight and optimal degree of branching when extracted by US in comparison with conventional methods.
27161557	3	4	theme	branching	659:667	arg1	degree					649:654	degree	649:654	degree of branching	649:667	Therefore three extraction methods i.e. hot water extraction (HWE), soxhlet extraction (SE) and ultrasound assisted extraction (US) were employed to study the total content of (1-3; 1-6)-β-d-glucans, degree of branching, structural characteristics, monosaccharides composition, as well as the total yield of polysaccharides that could be obtained from the artificially cultivated Ganoderma.
27161557	3	4	theme	branching	659:667	arg1	-β-d-glucans					635:646	(1-3; 1-6)-β-d-glucans	625:646	(1-3; 1-6)-β-d-glucans	625:646	Therefore three extraction methods i.e. hot water extraction (HWE), soxhlet extraction (SE) and ultrasound assisted extraction (US) were employed to study the total content of (1-3; 1-6)-β-d-glucans, degree of branching, structural characteristics, monosaccharides composition, as well as the total yield of polysaccharides that could be obtained from the artificially cultivated Ganoderma.
27161557	2	5	theme	polysaccharides	368:382	arg1	degree					323:328	degree	323:328	degree	323:328	The extraction methods, molecular weight and degree of branching of (1-3; 1-6)-β-d-glucan polysaccharides is of prime importance to determine its antioxidant bioactivity.
27161557	2	5	theme	polysaccharides	368:382	arg1	weight					312:317	molecular weight	302:317	molecular weight	302:317	The extraction methods, molecular weight and degree of branching of (1-3; 1-6)-β-d-glucan polysaccharides is of prime importance to determine its antioxidant bioactivity.
27161557	2	5	theme	polysaccharides	368:382	arg1	extraction					282:291	The extraction methods, molecular weight and degree	278:328	extraction	282:291	The extraction methods, molecular weight and degree of branching of (1-3; 1-6)-β-d-glucan polysaccharides is of prime importance to determine its antioxidant bioactivity.
27161557	3	6	theme	cultivated	818:827	arg1	Ganoderma					829:837	the artificially cultivated Ganoderma	801:837	the artificially cultivated Ganoderma	801:837	Therefore three extraction methods i.e. hot water extraction (HWE), soxhlet extraction (SE) and ultrasound assisted extraction (US) were employed to study the total content of (1-3; 1-6)-β-d-glucans, degree of branching, structural characteristics, monosaccharides composition, as well as the total yield of polysaccharides that could be obtained from the artificially cultivated Ganoderma.
27161557	4	7	theme	in	926:927	arg1	assays					935:940	the antioxidant in vitro assays	910:940	the antioxidant in vitro assays of DPPH scavenging activity and ferric reducing power (FRAP)	910:1001	The physical characteristics by HPAEC-PAD, HPGPC and FTIR, as well as the antioxidant in vitro assays of DPPH scavenging activity and ferric reducing power (FRAP) indicated that (1-3; 1-6)-β-d-glucans of Malaysian mushroom have better antioxidant activity, higher molecular weight and optimal degree of branching when extracted by US in comparison with conventional methods.
27161557	3	8	theme	assisted	556:563	arg1	extraction					565:574	ultrasound assisted extraction	545:574	ultrasound assisted extraction (US)	545:579	Therefore three extraction methods i.e. hot water extraction (HWE), soxhlet extraction (SE) and ultrasound assisted extraction (US) were employed to study the total content of (1-3; 1-6)-β-d-glucans, degree of branching, structural characteristics, monosaccharides composition, as well as the total yield of polysaccharides that could be obtained from the artificially cultivated Ganoderma.
27161557	2	9	theme	branching	333:341	arg1	degree					323:328	degree	323:328	degree	323:328	The extraction methods, molecular weight and degree of branching of (1-3; 1-6)-β-d-glucan polysaccharides is of prime importance to determine its antioxidant bioactivity.
27161557	2	9	theme	branching	333:341	arg1	weight					312:317	molecular weight	302:317	molecular weight	302:317	The extraction methods, molecular weight and degree of branching of (1-3; 1-6)-β-d-glucan polysaccharides is of prime importance to determine its antioxidant bioactivity.
27161557	2	9	theme	branching	333:341	arg1	extraction					282:291	The extraction methods, molecular weight and degree	278:328	extraction	282:291	The extraction methods, molecular weight and degree of branching of (1-3; 1-6)-β-d-glucan polysaccharides is of prime importance to determine its antioxidant bioactivity.
27161557	4	10	theme	antioxidant	914:924	arg1	assays					935:940	the antioxidant in vitro assays	910:940	the antioxidant in vitro assays of DPPH scavenging activity and ferric reducing power (FRAP)	910:1001	The physical characteristics by HPAEC-PAD, HPGPC and FTIR, as well as the antioxidant in vitro assays of DPPH scavenging activity and ferric reducing power (FRAP) indicated that (1-3; 1-6)-β-d-glucans of Malaysian mushroom have better antioxidant activity, higher molecular weight and optimal degree of branching when extracted by US in comparison with conventional methods.
27161557	4	11	theme	conventional	1193:1204	arg1	methods					1206:1212	conventional methods	1193:1212	conventional methods	1193:1212	The physical characteristics by HPAEC-PAD, HPGPC and FTIR, as well as the antioxidant in vitro assays of DPPH scavenging activity and ferric reducing power (FRAP) indicated that (1-3; 1-6)-β-d-glucans of Malaysian mushroom have better antioxidant activity, higher molecular weight and optimal degree of branching when extracted by US in comparison with conventional methods.
27161557	4	12	theme	antioxidant	1075:1085	arg1	activity					1087:1094	better antioxidant activity	1068:1094	better antioxidant activity	1068:1094	The physical characteristics by HPAEC-PAD, HPGPC and FTIR, as well as the antioxidant in vitro assays of DPPH scavenging activity and ferric reducing power (FRAP) indicated that (1-3; 1-6)-β-d-glucans of Malaysian mushroom have better antioxidant activity, higher molecular weight and optimal degree of branching when extracted by US in comparison with conventional methods.
27161557	1	13	theme	nutritional	210:220	arg1	fibers					222:227	chemically different nutritional fibers	189:227	chemically different nutritional fibers	189:227	Ganoderma mushroom cultivated recently in Malaysia to produce chemically different nutritional fibers has attracted the attention of the local market.
27161557	4	14	theme	ferric	974:979	arg1	FRAP					997:1000	FRAP	997:1000	FRAP	997:1000	The physical characteristics by HPAEC-PAD, HPGPC and FTIR, as well as the antioxidant in vitro assays of DPPH scavenging activity and ferric reducing power (FRAP) indicated that (1-3; 1-6)-β-d-glucans of Malaysian mushroom have better antioxidant activity, higher molecular weight and optimal degree of branching when extracted by US in comparison with conventional methods.
27161557	4	14	theme	ferric	974:979	arg1	power					990:994	ferric reducing power	974:994	ferric reducing power (FRAP)	974:1001	The physical characteristics by HPAEC-PAD, HPGPC and FTIR, as well as the antioxidant in vitro assays of DPPH scavenging activity and ferric reducing power (FRAP) indicated that (1-3; 1-6)-β-d-glucans of Malaysian mushroom have better antioxidant activity, higher molecular weight and optimal degree of branching when extracted by US in comparison with conventional methods.
27161557	3	15	theme	ultrasound	545:554	arg1	extraction					565:574	ultrasound assisted extraction	545:574	ultrasound assisted extraction (US)	545:579	Therefore three extraction methods i.e. hot water extraction (HWE), soxhlet extraction (SE) and ultrasound assisted extraction (US) were employed to study the total content of (1-3; 1-6)-β-d-glucans, degree of branching, structural characteristics, monosaccharides composition, as well as the total yield of polysaccharides that could be obtained from the artificially cultivated Ganoderma.
27161557	4	16	theme	higher	1097:1102	arg1	weight					1114:1119	higher molecular weight	1097:1119	higher molecular weight	1097:1119	The physical characteristics by HPAEC-PAD, HPGPC and FTIR, as well as the antioxidant in vitro assays of DPPH scavenging activity and ferric reducing power (FRAP) indicated that (1-3; 1-6)-β-d-glucans of Malaysian mushroom have better antioxidant activity, higher molecular weight and optimal degree of branching when extracted by US in comparison with conventional methods.
27161557	2	17	dep	extraction	282:291	arg1	methods					293:299	methods	293:299	methods	293:299	The extraction methods, molecular weight and degree of branching of (1-3; 1-6)-β-d-glucan polysaccharides is of prime importance to determine its antioxidant bioactivity.
27161557	4	18	theme	physical	844:851	arg1	characteristics					853:867	The physical characteristics	840:867	The physical characteristics by HPAEC-PAD, HPGPC and FTIR	840:896	The physical characteristics by HPAEC-PAD, HPGPC and FTIR, as well as the antioxidant in vitro assays of DPPH scavenging activity and ferric reducing power (FRAP) indicated that (1-3; 1-6)-β-d-glucans of Malaysian mushroom have better antioxidant activity, higher molecular weight and optimal degree of branching when extracted by US in comparison with conventional methods.
27161557	4	19	theme	mushroom	1054:1061	arg1	-β-d-glucans					1028:1039	(1-3; 1-6)-β-d-glucans	1018:1039	(1-3; 1-6)-β-d-glucans of Malaysian mushroom	1018:1061	The physical characteristics by HPAEC-PAD, HPGPC and FTIR, as well as the antioxidant in vitro assays of DPPH scavenging activity and ferric reducing power (FRAP) indicated that (1-3; 1-6)-β-d-glucans of Malaysian mushroom have better antioxidant activity, higher molecular weight and optimal degree of branching when extracted by US in comparison with conventional methods.
27161557	4	19	theme	mushroom	1054:1061	arg1	1-6					1024:1026	1-6	1024:1026	1-6	1024:1026	The physical characteristics by HPAEC-PAD, HPGPC and FTIR, as well as the antioxidant in vitro assays of DPPH scavenging activity and ferric reducing power (FRAP) indicated that (1-3; 1-6)-β-d-glucans of Malaysian mushroom have better antioxidant activity, higher molecular weight and optimal degree of branching when extracted by US in comparison with conventional methods.
27161557	3	20	theme	extraction	465:474	arg1	methods					476:482	three extraction methods	459:482	three extraction methods i.e. hot water extraction (HWE), soxhlet extraction (SE) and ultrasound assisted extraction (US)	459:579	Therefore three extraction methods i.e. hot water extraction (HWE), soxhlet extraction (SE) and ultrasound assisted extraction (US) were employed to study the total content of (1-3; 1-6)-β-d-glucans, degree of branching, structural characteristics, monosaccharides composition, as well as the total yield of polysaccharides that could be obtained from the artificially cultivated Ganoderma.
27161557	3	21	dep	extraction	499:508	arg1	i.e.					484:487	i.e.	484:487	i.e.	484:487	Therefore three extraction methods i.e. hot water extraction (HWE), soxhlet extraction (SE) and ultrasound assisted extraction (US) were employed to study the total content of (1-3; 1-6)-β-d-glucans, degree of branching, structural characteristics, monosaccharides composition, as well as the total yield of polysaccharides that could be obtained from the artificially cultivated Ganoderma.
27161557	4	22	contain	have	1063:1066	arg2	activity					1087:1094	better antioxidant activity	1068:1094	better antioxidant activity	1068:1094	The physical characteristics by HPAEC-PAD, HPGPC and FTIR, as well as the antioxidant in vitro assays of DPPH scavenging activity and ferric reducing power (FRAP) indicated that (1-3; 1-6)-β-d-glucans of Malaysian mushroom have better antioxidant activity, higher molecular weight and optimal degree of branching when extracted by US in comparison with conventional methods.
27161557	4	22	contain	have	1063:1066	arg1	1-6					1024:1026	1-6	1024:1026	1-6	1024:1026	The physical characteristics by HPAEC-PAD, HPGPC and FTIR, as well as the antioxidant in vitro assays of DPPH scavenging activity and ferric reducing power (FRAP) indicated that (1-3; 1-6)-β-d-glucans of Malaysian mushroom have better antioxidant activity, higher molecular weight and optimal degree of branching when extracted by US in comparison with conventional methods.
27161557	4	22	contain	have	1063:1066	arg1	-β-d-glucans					1028:1039	(1-3; 1-6)-β-d-glucans	1018:1039	(1-3; 1-6)-β-d-glucans of Malaysian mushroom	1018:1061	The physical characteristics by HPAEC-PAD, HPGPC and FTIR, as well as the antioxidant in vitro assays of DPPH scavenging activity and ferric reducing power (FRAP) indicated that (1-3; 1-6)-β-d-glucans of Malaysian mushroom have better antioxidant activity, higher molecular weight and optimal degree of branching when extracted by US in comparison with conventional methods.
27161557	4	22	contain	have	1063:1066	arg2	degree					1133:1138	optimal degree	1125:1138	optimal degree of branching	1125:1151	The physical characteristics by HPAEC-PAD, HPGPC and FTIR, as well as the antioxidant in vitro assays of DPPH scavenging activity and ferric reducing power (FRAP) indicated that (1-3; 1-6)-β-d-glucans of Malaysian mushroom have better antioxidant activity, higher molecular weight and optimal degree of branching when extracted by US in comparison with conventional methods.
27161557	4	22	contain	have	1063:1066	arg2	weight					1114:1119	higher molecular weight	1097:1119	higher molecular weight	1097:1119	The physical characteristics by HPAEC-PAD, HPGPC and FTIR, as well as the antioxidant in vitro assays of DPPH scavenging activity and ferric reducing power (FRAP) indicated that (1-3; 1-6)-β-d-glucans of Malaysian mushroom have better antioxidant activity, higher molecular weight and optimal degree of branching when extracted by US in comparison with conventional methods.
27161557	2	23	dep	-β-d-glucan	356:366	arg1	1-6					352:354	1-6	352:354	1-6	352:354	The extraction methods, molecular weight and degree of branching of (1-3; 1-6)-β-d-glucan polysaccharides is of prime importance to determine its antioxidant bioactivity.
27161557	4	24	theme	Malaysian	1044:1052	arg1	mushroom					1054:1061	Malaysian mushroom	1044:1061	Malaysian mushroom	1044:1061	The physical characteristics by HPAEC-PAD, HPGPC and FTIR, as well as the antioxidant in vitro assays of DPPH scavenging activity and ferric reducing power (FRAP) indicated that (1-3; 1-6)-β-d-glucans of Malaysian mushroom have better antioxidant activity, higher molecular weight and optimal degree of branching when extracted by US in comparison with conventional methods.
27161557	4	25	with	comparison	1177:1186	arg1	methods					1206:1212	conventional methods	1193:1212	conventional methods	1193:1212	The physical characteristics by HPAEC-PAD, HPGPC and FTIR, as well as the antioxidant in vitro assays of DPPH scavenging activity and ferric reducing power (FRAP) indicated that (1-3; 1-6)-β-d-glucans of Malaysian mushroom have better antioxidant activity, higher molecular weight and optimal degree of branching when extracted by US in comparison with conventional methods.
27161557	1	26	theme	different	200:208	arg1	fibers					222:227	chemically different nutritional fibers	189:227	chemically different nutritional fibers	189:227	Ganoderma mushroom cultivated recently in Malaysia to produce chemically different nutritional fibers has attracted the attention of the local market.
27161557	4	27	theme	better	1068:1073	arg1	activity					1087:1094	better antioxidant activity	1068:1094	better antioxidant activity	1068:1094	The physical characteristics by HPAEC-PAD, HPGPC and FTIR, as well as the antioxidant in vitro assays of DPPH scavenging activity and ferric reducing power (FRAP) indicated that (1-3; 1-6)-β-d-glucans of Malaysian mushroom have better antioxidant activity, higher molecular weight and optimal degree of branching when extracted by US in comparison with conventional methods.
27161557	0	28	theme	cultivated	54:63	arg1	mushroom					65:72	artificially cultivated mushroom	41:72	artificially cultivated mushroom	41:72	Ultrasonically extracted β-d-glucan from artificially cultivated mushroom, characteristic properties and antioxidant activity.
27161557	4	29	dep	in	926:927	arg1	vitro					929:933	vitro	929:933	vitro	929:933	The physical characteristics by HPAEC-PAD, HPGPC and FTIR, as well as the antioxidant in vitro assays of DPPH scavenging activity and ferric reducing power (FRAP) indicated that (1-3; 1-6)-β-d-glucans of Malaysian mushroom have better antioxidant activity, higher molecular weight and optimal degree of branching when extracted by US in comparison with conventional methods.
27161557	3	30	theme	hot	489:491	arg1	HWE					511:513	HWE	511:513	HWE	511:513	Therefore three extraction methods i.e. hot water extraction (HWE), soxhlet extraction (SE) and ultrasound assisted extraction (US) were employed to study the total content of (1-3; 1-6)-β-d-glucans, degree of branching, structural characteristics, monosaccharides composition, as well as the total yield of polysaccharides that could be obtained from the artificially cultivated Ganoderma.
27161557	3	30	theme	hot	489:491	arg1	extraction					499:508	hot water extraction	489:508	hot water extraction (HWE)	489:514	Therefore three extraction methods i.e. hot water extraction (HWE), soxhlet extraction (SE) and ultrasound assisted extraction (US) were employed to study the total content of (1-3; 1-6)-β-d-glucans, degree of branching, structural characteristics, monosaccharides composition, as well as the total yield of polysaccharides that could be obtained from the artificially cultivated Ganoderma.
27161557	3	31	theme	water	493:497	arg1	HWE					511:513	HWE	511:513	HWE	511:513	Therefore three extraction methods i.e. hot water extraction (HWE), soxhlet extraction (SE) and ultrasound assisted extraction (US) were employed to study the total content of (1-3; 1-6)-β-d-glucans, degree of branching, structural characteristics, monosaccharides composition, as well as the total yield of polysaccharides that could be obtained from the artificially cultivated Ganoderma.
27161557	3	31	theme	water	493:497	arg1	extraction					499:508	hot water extraction	489:508	hot water extraction (HWE)	489:514	Therefore three extraction methods i.e. hot water extraction (HWE), soxhlet extraction (SE) and ultrasound assisted extraction (US) were employed to study the total content of (1-3; 1-6)-β-d-glucans, degree of branching, structural characteristics, monosaccharides composition, as well as the total yield of polysaccharides that could be obtained from the artificially cultivated Ganoderma.
27161557	4	32	theme	power	990:994	arg1	assays					935:940	the antioxidant in vitro assays	910:940	the antioxidant in vitro assays of DPPH scavenging activity and ferric reducing power (FRAP)	910:1001	The physical characteristics by HPAEC-PAD, HPGPC and FTIR, as well as the antioxidant in vitro assays of DPPH scavenging activity and ferric reducing power (FRAP) indicated that (1-3; 1-6)-β-d-glucans of Malaysian mushroom have better antioxidant activity, higher molecular weight and optimal degree of branching when extracted by US in comparison with conventional methods.
27161557	4	32	theme	power	990:994	arg1	characteristics					853:867	The physical characteristics	840:867	The physical characteristics by HPAEC-PAD, HPGPC and FTIR	840:896	The physical characteristics by HPAEC-PAD, HPGPC and FTIR, as well as the antioxidant in vitro assays of DPPH scavenging activity and ferric reducing power (FRAP) indicated that (1-3; 1-6)-β-d-glucans of Malaysian mushroom have better antioxidant activity, higher molecular weight and optimal degree of branching when extracted by US in comparison with conventional methods.
27161557	3	33	theme	polysaccharides	757:771	arg1	yield					748:752	the total yield	738:752	the total yield of polysaccharides that could be obtained from the artificially cultivated Ganoderma	738:837	Therefore three extraction methods i.e. hot water extraction (HWE), soxhlet extraction (SE) and ultrasound assisted extraction (US) were employed to study the total content of (1-3; 1-6)-β-d-glucans, degree of branching, structural characteristics, monosaccharides composition, as well as the total yield of polysaccharides that could be obtained from the artificially cultivated Ganoderma.
27161557	3	33	theme	polysaccharides	757:771	arg1	composition					714:724	composition	714:724	composition	714:724	Therefore three extraction methods i.e. hot water extraction (HWE), soxhlet extraction (SE) and ultrasound assisted extraction (US) were employed to study the total content of (1-3; 1-6)-β-d-glucans, degree of branching, structural characteristics, monosaccharides composition, as well as the total yield of polysaccharides that could be obtained from the artificially cultivated Ganoderma.
27161557	3	33	theme	polysaccharides	757:771	arg1	-β-d-glucans					635:646	(1-3; 1-6)-β-d-glucans	625:646	(1-3; 1-6)-β-d-glucans	625:646	Therefore three extraction methods i.e. hot water extraction (HWE), soxhlet extraction (SE) and ultrasound assisted extraction (US) were employed to study the total content of (1-3; 1-6)-β-d-glucans, degree of branching, structural characteristics, monosaccharides composition, as well as the total yield of polysaccharides that could be obtained from the artificially cultivated Ganoderma.
27161557	2	34	theme	-β-d-glucan	356:366	arg1	polysaccharides					368:382	(1-3; 1-6)-β-d-glucan polysaccharides	346:382	(1-3; 1-6)-β-d-glucan polysaccharides	346:382	The extraction methods, molecular weight and degree of branching of (1-3; 1-6)-β-d-glucan polysaccharides is of prime importance to determine its antioxidant bioactivity.
27161557	4	35	theme	reducing	981:988	arg1	FRAP					997:1000	FRAP	997:1000	FRAP	997:1000	The physical characteristics by HPAEC-PAD, HPGPC and FTIR, as well as the antioxidant in vitro assays of DPPH scavenging activity and ferric reducing power (FRAP) indicated that (1-3; 1-6)-β-d-glucans of Malaysian mushroom have better antioxidant activity, higher molecular weight and optimal degree of branching when extracted by US in comparison with conventional methods.
27161557	4	35	theme	reducing	981:988	arg1	power					990:994	ferric reducing power	974:994	ferric reducing power (FRAP)	974:1001	The physical characteristics by HPAEC-PAD, HPGPC and FTIR, as well as the antioxidant in vitro assays of DPPH scavenging activity and ferric reducing power (FRAP) indicated that (1-3; 1-6)-β-d-glucans of Malaysian mushroom have better antioxidant activity, higher molecular weight and optimal degree of branching when extracted by US in comparison with conventional methods.
27161557	3	36	theme	total	742:746	arg1	yield					748:752	the total yield	738:752	the total yield of polysaccharides that could be obtained from the artificially cultivated Ganoderma	738:837	Therefore three extraction methods i.e. hot water extraction (HWE), soxhlet extraction (SE) and ultrasound assisted extraction (US) were employed to study the total content of (1-3; 1-6)-β-d-glucans, degree of branching, structural characteristics, monosaccharides composition, as well as the total yield of polysaccharides that could be obtained from the artificially cultivated Ganoderma.
27161557	3	36	theme	total	742:746	arg1	-β-d-glucans					635:646	(1-3; 1-6)-β-d-glucans	625:646	(1-3; 1-6)-β-d-glucans	625:646	Therefore three extraction methods i.e. hot water extraction (HWE), soxhlet extraction (SE) and ultrasound assisted extraction (US) were employed to study the total content of (1-3; 1-6)-β-d-glucans, degree of branching, structural characteristics, monosaccharides composition, as well as the total yield of polysaccharides that could be obtained from the artificially cultivated Ganoderma.
27161557	3	37	theme	soxhlet	517:523	arg1	SE					537:538	SE	537:538	SE	537:538	Therefore three extraction methods i.e. hot water extraction (HWE), soxhlet extraction (SE) and ultrasound assisted extraction (US) were employed to study the total content of (1-3; 1-6)-β-d-glucans, degree of branching, structural characteristics, monosaccharides composition, as well as the total yield of polysaccharides that could be obtained from the artificially cultivated Ganoderma.
27161557	3	37	theme	soxhlet	517:523	arg1	extraction					525:534	soxhlet extraction	517:534	soxhlet extraction (SE)	517:539	Therefore three extraction methods i.e. hot water extraction (HWE), soxhlet extraction (SE) and ultrasound assisted extraction (US) were employed to study the total content of (1-3; 1-6)-β-d-glucans, degree of branching, structural characteristics, monosaccharides composition, as well as the total yield of polysaccharides that could be obtained from the artificially cultivated Ganoderma.
27161557	2	38	theme	molecular	302:310	arg1	weight					312:317	molecular weight	302:317	molecular weight	302:317	The extraction methods, molecular weight and degree of branching of (1-3; 1-6)-β-d-glucan polysaccharides is of prime importance to determine its antioxidant bioactivity.
27161557	4	39	theme	branching	1143:1151	arg1	activity					1087:1094	better antioxidant activity	1068:1094	better antioxidant activity	1068:1094	The physical characteristics by HPAEC-PAD, HPGPC and FTIR, as well as the antioxidant in vitro assays of DPPH scavenging activity and ferric reducing power (FRAP) indicated that (1-3; 1-6)-β-d-glucans of Malaysian mushroom have better antioxidant activity, higher molecular weight and optimal degree of branching when extracted by US in comparison with conventional methods.
27161557	4	39	theme	branching	1143:1151	arg1	weight					1114:1119	higher molecular weight	1097:1119	higher molecular weight	1097:1119	The physical characteristics by HPAEC-PAD, HPGPC and FTIR, as well as the antioxidant in vitro assays of DPPH scavenging activity and ferric reducing power (FRAP) indicated that (1-3; 1-6)-β-d-glucans of Malaysian mushroom have better antioxidant activity, higher molecular weight and optimal degree of branching when extracted by US in comparison with conventional methods.
27161557	4	39	theme	branching	1143:1151	arg1	degree					1133:1138	optimal degree	1125:1138	optimal degree of branching	1125:1151	The physical characteristics by HPAEC-PAD, HPGPC and FTIR, as well as the antioxidant in vitro assays of DPPH scavenging activity and ferric reducing power (FRAP) indicated that (1-3; 1-6)-β-d-glucans of Malaysian mushroom have better antioxidant activity, higher molecular weight and optimal degree of branching when extracted by US in comparison with conventional methods.
27161557	1	40	theme	Ganoderma	127:135	arg1	mushroom					137:144	Ganoderma mushroom	127:144	Ganoderma mushroom cultivated recently in Malaysia to produce chemically different nutritional fibers	127:227	Ganoderma mushroom cultivated recently in Malaysia to produce chemically different nutritional fibers has attracted the attention of the local market.
27161557	1	41	theme	local	264:268	arg1	market					270:275	the local market	260:275	the local market	260:275	Ganoderma mushroom cultivated recently in Malaysia to produce chemically different nutritional fibers has attracted the attention of the local market.
27161557	3	42	theme	-β-d-glucans	635:646	arg1	content					614:620	the total content	604:620	the total content of (1-3; 1-6)-β-d-glucans, degree of branching, structural characteristics, monosaccharides composition, as well as the total yield of polysaccharides that could be obtained from the artificially cultivated Ganoderma	604:837	Therefore three extraction methods i.e. hot water extraction (HWE), soxhlet extraction (SE) and ultrasound assisted extraction (US) were employed to study the total content of (1-3; 1-6)-β-d-glucans, degree of branching, structural characteristics, monosaccharides composition, as well as the total yield of polysaccharides that could be obtained from the artificially cultivated Ganoderma.
27161557	3	43	dep	extraction	565:574	arg1	US					577:578	US	577:578	US	577:578	Therefore three extraction methods i.e. hot water extraction (HWE), soxhlet extraction (SE) and ultrasound assisted extraction (US) were employed to study the total content of (1-3; 1-6)-β-d-glucans, degree of branching, structural characteristics, monosaccharides composition, as well as the total yield of polysaccharides that could be obtained from the artificially cultivated Ganoderma.
27161557	1	44	theme	market	270:275	arg1	attention					247:255	the attention	243:255	the attention of the local market	243:275	Ganoderma mushroom cultivated recently in Malaysia to produce chemically different nutritional fibers has attracted the attention of the local market.
27161557	4	45	theme	DPPH	945:948	arg1	activity					961:968	DPPH scavenging activity	945:968	DPPH scavenging activity	945:968	The physical characteristics by HPAEC-PAD, HPGPC and FTIR, as well as the antioxidant in vitro assays of DPPH scavenging activity and ferric reducing power (FRAP) indicated that (1-3; 1-6)-β-d-glucans of Malaysian mushroom have better antioxidant activity, higher molecular weight and optimal degree of branching when extracted by US in comparison with conventional methods.
27161557	2	46	theme	antioxidant	424:434	arg1	bioactivity					436:446	its antioxidant bioactivity	420:446	its antioxidant bioactivity	420:446	The extraction methods, molecular weight and degree of branching of (1-3; 1-6)-β-d-glucan polysaccharides is of prime importance to determine its antioxidant bioactivity.
27161557	3	47	theme	total	608:612	arg1	content					614:620	the total content	604:620	the total content of (1-3; 1-6)-β-d-glucans, degree of branching, structural characteristics, monosaccharides composition, as well as the total yield of polysaccharides that could be obtained from the artificially cultivated Ganoderma	604:837	Therefore three extraction methods i.e. hot water extraction (HWE), soxhlet extraction (SE) and ultrasound assisted extraction (US) were employed to study the total content of (1-3; 1-6)-β-d-glucans, degree of branching, structural characteristics, monosaccharides composition, as well as the total yield of polysaccharides that could be obtained from the artificially cultivated Ganoderma.
27161557	4	48	theme	molecular	1104:1112	arg1	weight					1114:1119	higher molecular weight	1097:1119	higher molecular weight	1097:1119	The physical characteristics by HPAEC-PAD, HPGPC and FTIR, as well as the antioxidant in vitro assays of DPPH scavenging activity and ferric reducing power (FRAP) indicated that (1-3; 1-6)-β-d-glucans of Malaysian mushroom have better antioxidant activity, higher molecular weight and optimal degree of branching when extracted by US in comparison with conventional methods.
27161557	2	49	theme	prime	390:394	arg1	importance					396:405	prime importance	390:405	prime importance	390:405	The extraction methods, molecular weight and degree of branching of (1-3; 1-6)-β-d-glucan polysaccharides is of prime importance to determine its antioxidant bioactivity.
27161557	0	50	theme	characteristic	75:88	arg1	properties					90:99	characteristic properties	75:99	characteristic properties	75:99	Ultrasonically extracted β-d-glucan from artificially cultivated mushroom, characteristic properties and antioxidant activity.
27161557	3	51	theme	structural	670:679	arg1	characteristics					681:695	structural characteristics	670:695	structural characteristics	670:695	Therefore three extraction methods i.e. hot water extraction (HWE), soxhlet extraction (SE) and ultrasound assisted extraction (US) were employed to study the total content of (1-3; 1-6)-β-d-glucans, degree of branching, structural characteristics, monosaccharides composition, as well as the total yield of polysaccharides that could be obtained from the artificially cultivated Ganoderma.
27161557	3	51	theme	structural	670:679	arg1	-β-d-glucans					635:646	(1-3; 1-6)-β-d-glucans	625:646	(1-3; 1-6)-β-d-glucans	625:646	Therefore three extraction methods i.e. hot water extraction (HWE), soxhlet extraction (SE) and ultrasound assisted extraction (US) were employed to study the total content of (1-3; 1-6)-β-d-glucans, degree of branching, structural characteristics, monosaccharides composition, as well as the total yield of polysaccharides that could be obtained from the artificially cultivated Ganoderma.
27161557	4	52	theme	optimal	1125:1131	arg1	degree					1133:1138	optimal degree	1125:1138	optimal degree of branching	1125:1151	The physical characteristics by HPAEC-PAD, HPGPC and FTIR, as well as the antioxidant in vitro assays of DPPH scavenging activity and ferric reducing power (FRAP) indicated that (1-3; 1-6)-β-d-glucans of Malaysian mushroom have better antioxidant activity, higher molecular weight and optimal degree of branching when extracted by US in comparison with conventional methods.
28768658	4	0	theme	hedonics	1011:1018	arg1	irrespective					969:980	irrespective	969:980	irrespective	969:980	Therefore, mice lacking T1R2 + T1R3 and wild-type controls were tested in a two-response taste discrimination task to determine whether maltodextrins are 1) detectable when both receptor subunits are absent and 2) perceptually distinct from that of sucrose irrespective of viscosity, intensity, and hedonics.
28768658	2	1	theme	taste	307:311	arg1	receptor					313:320	the T1R2 + T1R3 "sweet" taste receptor	283:320	the T1R2 + T1R3 "sweet" taste receptor	283:320	Although glucose and other sugars activate the T1R2 + T1R3 "sweet" taste receptor, recent evidence from T1R2- or T1R3-knockout (KO) mice suggests that maltodextrins, despite their glucose polymer composition, activate a separate receptor mechanism to generate a taste percept qualitatively distinguishable from that of sweeteners.
28768658	7	2	theme	hedonic	1467:1473	arg1	value					1475:1479	hedonic value	1467:1479	hedonic value of the solutions irrelevant	1467:1507	Varied Maltrin and sucrose concentrations of approximately matched viscosities were then presented to render the oral somatosensory features, intensity, and hedonic value of the solutions irrelevant.
28768658	6	3	theme	Polycose	1142:1149	arg1	concentrations					1151:1164	the higher Polycose concentrations	1131:1164	the higher Polycose concentrations	1131:1164	However, some KO mice were only sensitive to the higher Polycose concentrations, implicating potential allelic variation in the putative polysaccharide receptor or downstream pathways unmasked by the absence of T1R2 + T1R3.
28768658	6	4	theme	downstream	1250:1259	arg1	pathways					1261:1268	downstream pathways	1250:1268	downstream pathways unmasked by the absence of T1R2 + T1R3	1250:1307	However, some KO mice were only sensitive to the higher Polycose concentrations, implicating potential allelic variation in the putative polysaccharide receptor or downstream pathways unmasked by the absence of T1R2 + T1R3.
28768658	2	5	theme	other	261:265	arg1	sugars					267:272	other sugars	261:272	other sugars	261:272	Although glucose and other sugars activate the T1R2 + T1R3 "sweet" taste receptor, recent evidence from T1R2- or T1R3-knockout (KO) mice suggests that maltodextrins, despite their glucose polymer composition, activate a separate receptor mechanism to generate a taste percept qualitatively distinguishable from that of sweeteners.
28768658	9	6	theme	distinguishable	1873:1887	arg1	sensation					1889:1897	a qualitatively distinguishable sensation	1857:1897	a qualitatively distinguishable sensation from that of sucrose	1857:1918	These data support the proposed presence of an orosensory receptor mechanism that gives rise to a qualitatively distinguishable sensation from that of sucrose.
28768658	6	7	theme	putative	1214:1221	arg1	receptor					1238:1245	the putative polysaccharide receptor	1210:1245	the putative polysaccharide receptor	1210:1245	However, some KO mice were only sensitive to the higher Polycose concentrations, implicating potential allelic variation in the putative polysaccharide receptor or downstream pathways unmasked by the absence of T1R2 + T1R3.
28768658	2	8	theme	T1R2	287:290	arg1	receptor					313:320	the T1R2 + T1R3 "sweet" taste receptor	283:320	the T1R2 + T1R3 "sweet" taste receptor	283:320	Although glucose and other sugars activate the T1R2 + T1R3 "sweet" taste receptor, recent evidence from T1R2- or T1R3-knockout (KO) mice suggests that maltodextrins, despite their glucose polymer composition, activate a separate receptor mechanism to generate a taste percept qualitatively distinguishable from that of sweeteners.
28768658	8	9	theme	sucrose	1726:1732	arg1	cue					1745:1747	sucrose orosensory cue	1726:1747	a Maltrin but not sucrose orosensory cue	1708:1747	Although both genotypes competently discriminated Maltrin from sucrose, performance was apparently driven by the different orosensory percepts of the two stimuli in control mice and the presence of a Maltrin but not sucrose orosensory cue in KO mice.
28768658	1	10	theme	polymer	167:173	arg1	Maltrin					133:139	Maltrin	133:139	Maltrin	133:139	Maltodextrins, such as Maltrin and Polycose, are glucose polymer mixtures of varying chain lengths that are palatable to rodents.
28768658	1	10	theme	polymer	167:173	arg1	Maltodextrins					110:122	Maltodextrins	110:122	Maltodextrins	110:122	Maltodextrins, such as Maltrin and Polycose, are glucose polymer mixtures of varying chain lengths that are palatable to rodents.
28768658	1	10	theme	polymer	167:173	arg1	mixtures					175:182	glucose polymer mixtures	159:182	glucose polymer mixtures of varying chain lengths that are palatable to rodents	159:237	Maltodextrins, such as Maltrin and Polycose, are glucose polymer mixtures of varying chain lengths that are palatable to rodents.
28768658	1	10	theme	polymer	167:173	arg1	Polycose					145:152	Polycose	145:152	Polycose	145:152	Maltodextrins, such as Maltrin and Polycose, are glucose polymer mixtures of varying chain lengths that are palatable to rodents.
28768658	2	11	theme	taste	502:506	arg1	percept					508:514	a taste percept	500:514	a taste percept qualitatively distinguishable from that of sweeteners	500:568	Although glucose and other sugars activate the T1R2 + T1R3 "sweet" taste receptor, recent evidence from T1R2- or T1R3-knockout (KO) mice suggests that maltodextrins, despite their glucose polymer composition, activate a separate receptor mechanism to generate a taste percept qualitatively distinguishable from that of sweeteners.
28768658	8	12	theme	cue	1745:1747	arg1	percepts					1644:1651	the different orosensory percepts	1619:1651	the different orosensory percepts of the two stimuli in control mice	1619:1686	Although both genotypes competently discriminated Maltrin from sucrose, performance was apparently driven by the different orosensory percepts of the two stimuli in control mice and the presence of a Maltrin but not sucrose orosensory cue in KO mice.
28768658	8	12	theme	cue	1745:1747	arg1	presence					1696:1703	the presence	1692:1703	the presence of a Maltrin but not sucrose orosensory cue in KO mice	1692:1758	Although both genotypes competently discriminated Maltrin from sucrose, performance was apparently driven by the different orosensory percepts of the two stimuli in control mice and the presence of a Maltrin but not sucrose orosensory cue in KO mice.
28768658	7	13	theme	matched	1369:1375	arg1	viscosities					1377:1387	approximately matched viscosities	1355:1387	approximately matched viscosities	1355:1387	Varied Maltrin and sucrose concentrations of approximately matched viscosities were then presented to render the oral somatosensory features, intensity, and hedonic value of the solutions irrelevant.
28768658	5	14	theme	similar	1044:1050	arg1	controls					1076:1083	controls	1076:1083	controls	1076:1083	Most KO mice displayed similar Polycose sensitivity as controls.
28768658	5	14	theme	similar	1044:1050	arg1	sensitivity					1061:1071	similar Polycose sensitivity	1044:1071	similar Polycose sensitivity	1044:1071	Most KO mice displayed similar Polycose sensitivity as controls.
28768658	8	15	theme	KO	1752:1753	arg1	mice					1755:1758	KO mice	1752:1758	KO mice	1752:1758	Although both genotypes competently discriminated Maltrin from sucrose, performance was apparently driven by the different orosensory percepts of the two stimuli in control mice and the presence of a Maltrin but not sucrose orosensory cue in KO mice.
28768658	1	16	theme	chain	195:199	arg1	lengths					201:207	varying chain lengths	187:207	varying chain lengths that are palatable to rodents	187:237	Maltodextrins, such as Maltrin and Polycose, are glucose polymer mixtures of varying chain lengths that are palatable to rodents.
28768658	7	17	theme	sucrose	1329:1335	arg1	concentrations					1337:1350	Varied Maltrin and sucrose concentrations	1310:1350	concentrations	1337:1350	Varied Maltrin and sucrose concentrations of approximately matched viscosities were then presented to render the oral somatosensory features, intensity, and hedonic value of the solutions irrelevant.
28768658	6	18	theme	potential	1179:1187	arg1	variation					1197:1205	potential allelic variation	1179:1205	potential allelic variation in the putative polysaccharide receptor	1179:1245	However, some KO mice were only sensitive to the higher Polycose concentrations, implicating potential allelic variation in the putative polysaccharide receptor or downstream pathways unmasked by the absence of T1R2 + T1R3.
28768658	5	19	theme	Most	1021:1024	arg1	mice					1029:1032	Most KO mice	1021:1032	Most KO mice	1021:1032	Most KO mice displayed similar Polycose sensitivity as controls.
28768658	7	20	theme	oral	1423:1426	arg1	features					1442:1449	the oral somatosensory features	1419:1449	the oral somatosensory features	1419:1449	Varied Maltrin and sucrose concentrations of approximately matched viscosities were then presented to render the oral somatosensory features, intensity, and hedonic value of the solutions irrelevant.
28768658	8	21	attach	presence	1696:1703	arg2	cue					1745:1747	sucrose orosensory cue	1726:1747	a Maltrin but not sucrose orosensory cue	1708:1747	Although both genotypes competently discriminated Maltrin from sucrose, performance was apparently driven by the different orosensory percepts of the two stimuli in control mice and the presence of a Maltrin but not sucrose orosensory cue in KO mice.
28768658	8	21	attach	presence	1696:1703	arg1	mice					1755:1758	KO mice	1752:1758	KO mice	1752:1758	Although both genotypes competently discriminated Maltrin from sucrose, performance was apparently driven by the different orosensory percepts of the two stimuli in control mice and the presence of a Maltrin but not sucrose orosensory cue in KO mice.
28768658	8	21	attach	presence	1696:1703	arg2	stimuli					1664:1670	the two stimuli	1656:1670	the two stimuli in control mice	1656:1686	Although both genotypes competently discriminated Maltrin from sucrose, performance was apparently driven by the different orosensory percepts of the two stimuli in control mice and the presence of a Maltrin but not sucrose orosensory cue in KO mice.
28768658	8	21	attach	presence	1696:1703	arg2	Maltrin					1710:1716	a Maltrin	1708:1716	a Maltrin but not sucrose orosensory cue	1708:1747	Although both genotypes competently discriminated Maltrin from sucrose, performance was apparently driven by the different orosensory percepts of the two stimuli in control mice and the presence of a Maltrin but not sucrose orosensory cue in KO mice.
28768658	9	22	from	that	1904:1907	arg1	sensation					1889:1897	a qualitatively distinguishable sensation	1857:1897	a qualitatively distinguishable sensation from that of sucrose	1857:1918	These data support the proposed presence of an orosensory receptor mechanism that gives rise to a qualitatively distinguishable sensation from that of sucrose.
28768658	2	23	from	mice	372:375	arg1	evidence					330:337	recent evidence	323:337	recent evidence from T1R2- or T1R3-knockout (KO) mice	323:375	Although glucose and other sugars activate the T1R2 + T1R3 "sweet" taste receptor, recent evidence from T1R2- or T1R3-knockout (KO) mice suggests that maltodextrins, despite their glucose polymer composition, activate a separate receptor mechanism to generate a taste percept qualitatively distinguishable from that of sweeteners.
28768658	0	24	theme	T1R2	85:88	arg1	heterodimer					97:107	the T1R2 + T1R3 heterodimer	81:107	heterodimer	97:107	Detection of maltodextrin and its discrimination from sucrose are independent of the T1R2 + T1R3 heterodimer.
28768658	6	25	theme	higher	1135:1140	arg1	concentrations					1151:1164	the higher Polycose concentrations	1131:1164	the higher Polycose concentrations	1131:1164	However, some KO mice were only sensitive to the higher Polycose concentrations, implicating potential allelic variation in the putative polysaccharide receptor or downstream pathways unmasked by the absence of T1R2 + T1R3.
28768658	2	26	theme	separate	460:467	arg1	mechanism					478:486	a separate receptor mechanism	458:486	a separate receptor mechanism to generate a taste percept qualitatively distinguishable from that of sweeteners	458:568	Although glucose and other sugars activate the T1R2 + T1R3 "sweet" taste receptor, recent evidence from T1R2- or T1R3-knockout (KO) mice suggests that maltodextrins, despite their glucose polymer composition, activate a separate receptor mechanism to generate a taste percept qualitatively distinguishable from that of sweeteners.
28768658	8	27	from	stimuli	1664:1670	arg1	mice					1683:1686	control mice	1675:1686	control mice	1675:1686	Although both genotypes competently discriminated Maltrin from sucrose, performance was apparently driven by the different orosensory percepts of the two stimuli in control mice and the presence of a Maltrin but not sucrose orosensory cue in KO mice.
28768658	0	28	theme	T1R3	92:95	arg1	heterodimer					97:107	the T1R2 + T1R3 heterodimer	81:107	heterodimer	97:107	Detection of maltodextrin and its discrimination from sucrose are independent of the T1R2 + T1R3 heterodimer.
28768658	2	29	theme	glucose	420:426	arg1	composition					436:446	their glucose polymer composition	414:446	their glucose polymer composition	414:446	Although glucose and other sugars activate the T1R2 + T1R3 "sweet" taste receptor, recent evidence from T1R2- or T1R3-knockout (KO) mice suggests that maltodextrins, despite their glucose polymer composition, activate a separate receptor mechanism to generate a taste percept qualitatively distinguishable from that of sweeteners.
28768658	7	30	theme	Varied	1310:1315	arg1	Maltrin					1317:1323	Varied Maltrin and sucrose concentrations	1310:1350	Maltrin	1317:1323	Varied Maltrin and sucrose concentrations of approximately matched viscosities were then presented to render the oral somatosensory features, intensity, and hedonic value of the solutions irrelevant.
28768658	4	31	theme	intensity	996:1004	arg1	irrespective					969:980	irrespective	969:980	irrespective	969:980	Therefore, mice lacking T1R2 + T1R3 and wild-type controls were tested in a two-response taste discrimination task to determine whether maltodextrins are 1) detectable when both receptor subunits are absent and 2) perceptually distinct from that of sucrose irrespective of viscosity, intensity, and hedonics.
28768658	8	32	from	presence	1696:1703	arg1	mice					1755:1758	KO mice	1752:1758	KO mice	1752:1758	Although both genotypes competently discriminated Maltrin from sucrose, performance was apparently driven by the different orosensory percepts of the two stimuli in control mice and the presence of a Maltrin but not sucrose orosensory cue in KO mice.
28768658	8	32	from	presence	1696:1703	arg1	mice					1683:1686	control mice	1675:1686	control mice	1675:1686	Although both genotypes competently discriminated Maltrin from sucrose, performance was apparently driven by the different orosensory percepts of the two stimuli in control mice and the presence of a Maltrin but not sucrose orosensory cue in KO mice.
28768658	3	33	from	discrimination	589:602	arg1	sweeteners					639:648	prototypical sweeteners	626:648	prototypical sweeteners	626:648	However, explicit discrimination of maltodextrins from prototypical sweeteners has not yet been psychophysically tested in any murine model.
28768658	9	34	theme	orosensory	1808:1817	arg1	mechanism					1828:1836	an orosensory receptor mechanism	1805:1836	an orosensory receptor mechanism that gives rise to a qualitatively distinguishable sensation from that of sucrose	1805:1918	These data support the proposed presence of an orosensory receptor mechanism that gives rise to a qualitatively distinguishable sensation from that of sucrose.
28768658	2	35	from	that	551:554	arg1	distinguishable					530:544	distinguishable	530:544	distinguishable	530:544	Although glucose and other sugars activate the T1R2 + T1R3 "sweet" taste receptor, recent evidence from T1R2- or T1R3-knockout (KO) mice suggests that maltodextrins, despite their glucose polymer composition, activate a separate receptor mechanism to generate a taste percept qualitatively distinguishable from that of sweeteners.
28768658	0	36	theme	maltodextrin	13:24	arg1	discrimination					34:47	its discrimination	30:47	its discrimination from sucrose	30:60	Detection of maltodextrin and its discrimination from sucrose are independent of the T1R2 + T1R3 heterodimer.
28768658	0	36	theme	maltodextrin	13:24	arg1	Detection					0:8	Detection	0:8	Detection of maltodextrin	0:24	Detection of maltodextrin and its discrimination from sucrose are independent of the T1R2 + T1R3 heterodimer.
28768658	4	37	theme	irrespective	969:980	arg1	sucrose					961:967	sucrose	961:967	sucrose irrespective of viscosity, intensity, and hedonics	961:1018	Therefore, mice lacking T1R2 + T1R3 and wild-type controls were tested in a two-response taste discrimination task to determine whether maltodextrins are 1) detectable when both receptor subunits are absent and 2) perceptually distinct from that of sucrose irrespective of viscosity, intensity, and hedonics.
28768658	3	38	theme	prototypical	626:637	arg1	sweeteners					639:648	prototypical sweeteners	626:648	prototypical sweeteners	626:648	However, explicit discrimination of maltodextrins from prototypical sweeteners has not yet been psychophysically tested in any murine model.
28768658	4	39	theme	viscosity	985:993	arg1	irrespective					969:980	irrespective	969:980	irrespective	969:980	Therefore, mice lacking T1R2 + T1R3 and wild-type controls were tested in a two-response taste discrimination task to determine whether maltodextrins are 1) detectable when both receptor subunits are absent and 2) perceptually distinct from that of sucrose irrespective of viscosity, intensity, and hedonics.
28768658	3	40	theme	maltodextrins	607:619	arg1	discrimination					589:602	explicit discrimination	580:602	explicit discrimination of maltodextrins from prototypical sweeteners	580:648	However, explicit discrimination of maltodextrins from prototypical sweeteners has not yet been psychophysically tested in any murine model.
28768658	4	41	theme	taste	801:805	arg1	task					822:825	a two-response taste discrimination task	786:825	a two-response taste discrimination task	786:825	Therefore, mice lacking T1R2 + T1R3 and wild-type controls were tested in a two-response taste discrimination task to determine whether maltodextrins are 1) detectable when both receptor subunits are absent and 2) perceptually distinct from that of sucrose irrespective of viscosity, intensity, and hedonics.
28768658	2	42	theme	"	305:305	arg1	receptor					313:320	the T1R2 + T1R3 "sweet" taste receptor	283:320	the T1R2 + T1R3 "sweet" taste receptor	283:320	Although glucose and other sugars activate the T1R2 + T1R3 "sweet" taste receptor, recent evidence from T1R2- or T1R3-knockout (KO) mice suggests that maltodextrins, despite their glucose polymer composition, activate a separate receptor mechanism to generate a taste percept qualitatively distinguishable from that of sweeteners.
28768658	7	43	theme	irrelevant	1498:1507	arg1	solutions					1488:1496	the solutions	1484:1496	the solutions irrelevant	1484:1507	Varied Maltrin and sucrose concentrations of approximately matched viscosities were then presented to render the oral somatosensory features, intensity, and hedonic value of the solutions irrelevant.
28768658	1	44	theme	lengths	201:207	arg1	Maltrin					133:139	Maltrin	133:139	Maltrin	133:139	Maltodextrins, such as Maltrin and Polycose, are glucose polymer mixtures of varying chain lengths that are palatable to rodents.
28768658	1	44	theme	lengths	201:207	arg1	Maltodextrins					110:122	Maltodextrins	110:122	Maltodextrins	110:122	Maltodextrins, such as Maltrin and Polycose, are glucose polymer mixtures of varying chain lengths that are palatable to rodents.
28768658	1	44	theme	lengths	201:207	arg1	mixtures					175:182	glucose polymer mixtures	159:182	glucose polymer mixtures of varying chain lengths that are palatable to rodents	159:237	Maltodextrins, such as Maltrin and Polycose, are glucose polymer mixtures of varying chain lengths that are palatable to rodents.
28768658	1	44	theme	lengths	201:207	arg1	Polycose					145:152	Polycose	145:152	Polycose	145:152	Maltodextrins, such as Maltrin and Polycose, are glucose polymer mixtures of varying chain lengths that are palatable to rodents.
28768658	3	45	from	sweeteners	639:648	arg1	maltodextrins					607:619	maltodextrins	607:619	maltodextrins from prototypical sweeteners	607:648	However, explicit discrimination of maltodextrins from prototypical sweeteners has not yet been psychophysically tested in any murine model.
28768658	3	45	from	sweeteners	639:648	arg1	discrimination					589:602	explicit discrimination	580:602	explicit discrimination of maltodextrins from prototypical sweeteners	580:648	However, explicit discrimination of maltodextrins from prototypical sweeteners has not yet been psychophysically tested in any murine model.
28768658	2	46	theme	recent	323:328	arg1	evidence					330:337	recent evidence	323:337	recent evidence from T1R2- or T1R3-knockout (KO) mice	323:375	Although glucose and other sugars activate the T1R2 + T1R3 "sweet" taste receptor, recent evidence from T1R2- or T1R3-knockout (KO) mice suggests that maltodextrins, despite their glucose polymer composition, activate a separate receptor mechanism to generate a taste percept qualitatively distinguishable from that of sweeteners.
28768658	9	47	theme	mechanism	1828:1836	arg1	presence					1793:1800	the proposed presence	1780:1800	the proposed presence of an orosensory receptor mechanism that gives rise to a qualitatively distinguishable sensation from that of sucrose	1780:1918	These data support the proposed presence of an orosensory receptor mechanism that gives rise to a qualitatively distinguishable sensation from that of sucrose.
28768658	8	48	theme	orosensory	1633:1642	arg1	percepts					1644:1651	the different orosensory percepts	1619:1651	the different orosensory percepts of the two stimuli in control mice	1619:1686	Although both genotypes competently discriminated Maltrin from sucrose, performance was apparently driven by the different orosensory percepts of the two stimuli in control mice and the presence of a Maltrin but not sucrose orosensory cue in KO mice.
28768658	7	49	theme	somatosensory	1428:1440	arg1	features					1442:1449	the oral somatosensory features	1419:1449	the oral somatosensory features	1419:1449	Varied Maltrin and sucrose concentrations of approximately matched viscosities were then presented to render the oral somatosensory features, intensity, and hedonic value of the solutions irrelevant.
28768658	4	50	theme	discrimination	807:820	arg1	task					822:825	a two-response taste discrimination task	786:825	a two-response taste discrimination task	786:825	Therefore, mice lacking T1R2 + T1R3 and wild-type controls were tested in a two-response taste discrimination task to determine whether maltodextrins are 1) detectable when both receptor subunits are absent and 2) perceptually distinct from that of sucrose irrespective of viscosity, intensity, and hedonics.
28768658	3	51	theme	murine	698:703	arg1	model					705:709	any murine model	694:709	any murine model	694:709	However, explicit discrimination of maltodextrins from prototypical sweeteners has not yet been psychophysically tested in any murine model.
28768658	6	52	theme	polysaccharide	1223:1236	arg1	receptor					1238:1245	the putative polysaccharide receptor	1210:1245	the putative polysaccharide receptor	1210:1245	However, some KO mice were only sensitive to the higher Polycose concentrations, implicating potential allelic variation in the putative polysaccharide receptor or downstream pathways unmasked by the absence of T1R2 + T1R3.
28768658	8	53	theme	Maltrin	1710:1716	arg1	percepts					1644:1651	the different orosensory percepts	1619:1651	the different orosensory percepts of the two stimuli in control mice	1619:1686	Although both genotypes competently discriminated Maltrin from sucrose, performance was apparently driven by the different orosensory percepts of the two stimuli in control mice and the presence of a Maltrin but not sucrose orosensory cue in KO mice.
28768658	8	53	theme	Maltrin	1710:1716	arg1	presence					1696:1703	the presence	1692:1703	the presence of a Maltrin but not sucrose orosensory cue in KO mice	1692:1758	Although both genotypes competently discriminated Maltrin from sucrose, performance was apparently driven by the different orosensory percepts of the two stimuli in control mice and the presence of a Maltrin but not sucrose orosensory cue in KO mice.
28768658	1	54	theme	glucose	159:165	arg1	Maltrin					133:139	Maltrin	133:139	Maltrin	133:139	Maltodextrins, such as Maltrin and Polycose, are glucose polymer mixtures of varying chain lengths that are palatable to rodents.
28768658	1	54	theme	glucose	159:165	arg1	Maltodextrins					110:122	Maltodextrins	110:122	Maltodextrins	110:122	Maltodextrins, such as Maltrin and Polycose, are glucose polymer mixtures of varying chain lengths that are palatable to rodents.
28768658	1	54	theme	glucose	159:165	arg1	mixtures					175:182	glucose polymer mixtures	159:182	glucose polymer mixtures of varying chain lengths that are palatable to rodents	159:237	Maltodextrins, such as Maltrin and Polycose, are glucose polymer mixtures of varying chain lengths that are palatable to rodents.
28768658	1	54	theme	glucose	159:165	arg1	Polycose					145:152	Polycose	145:152	Polycose	145:152	Maltodextrins, such as Maltrin and Polycose, are glucose polymer mixtures of varying chain lengths that are palatable to rodents.
28768658	4	55	theme	receptor	890:897	arg1	subunits					899:906	both receptor subunits	885:906	both receptor subunits	885:906	Therefore, mice lacking T1R2 + T1R3 and wild-type controls were tested in a two-response taste discrimination task to determine whether maltodextrins are 1) detectable when both receptor subunits are absent and 2) perceptually distinct from that of sucrose irrespective of viscosity, intensity, and hedonics.
28768658	6	56	theme	T1R3	1304:1307	arg1	absence					1286:1292	the absence	1282:1292	the absence of T1R2 + T1R3	1282:1307	However, some KO mice were only sensitive to the higher Polycose concentrations, implicating potential allelic variation in the putative polysaccharide receptor or downstream pathways unmasked by the absence of T1R2 + T1R3.
28768658	8	57	theme	orosensory	1734:1743	arg1	cue					1745:1747	sucrose orosensory cue	1726:1747	a Maltrin but not sucrose orosensory cue	1708:1747	Although both genotypes competently discriminated Maltrin from sucrose, performance was apparently driven by the different orosensory percepts of the two stimuli in control mice and the presence of a Maltrin but not sucrose orosensory cue in KO mice.
28768658	1	58	theme	varying	187:193	arg1	lengths					201:207	varying chain lengths	187:207	varying chain lengths that are palatable to rodents	187:237	Maltodextrins, such as Maltrin and Polycose, are glucose polymer mixtures of varying chain lengths that are palatable to rodents.
28768658	7	59	theme	viscosities	1377:1387	arg1	Maltrin					1317:1323	Varied Maltrin and sucrose concentrations	1310:1350	Maltrin	1317:1323	Varied Maltrin and sucrose concentrations of approximately matched viscosities were then presented to render the oral somatosensory features, intensity, and hedonic value of the solutions irrelevant.
28768658	7	59	theme	viscosities	1377:1387	arg1	concentrations					1337:1350	Varied Maltrin and sucrose concentrations	1310:1350	concentrations	1337:1350	Varied Maltrin and sucrose concentrations of approximately matched viscosities were then presented to render the oral somatosensory features, intensity, and hedonic value of the solutions irrelevant.
28768658	6	60	theme	T1R2	1297:1300	arg1	absence					1286:1292	the absence	1282:1292	the absence of T1R2 + T1R3	1282:1307	However, some KO mice were only sensitive to the higher Polycose concentrations, implicating potential allelic variation in the putative polysaccharide receptor or downstream pathways unmasked by the absence of T1R2 + T1R3.
28768658	5	61	theme	Polycose	1052:1059	arg1	controls					1076:1083	controls	1076:1083	controls	1076:1083	Most KO mice displayed similar Polycose sensitivity as controls.
28768658	5	61	theme	Polycose	1052:1059	arg1	sensitivity					1061:1071	similar Polycose sensitivity	1044:1071	similar Polycose sensitivity	1044:1071	Most KO mice displayed similar Polycose sensitivity as controls.
28768658	2	62	theme	distinguishable	530:544	arg1	percept					508:514	a taste percept	500:514	a taste percept qualitatively distinguishable from that of sweeteners	500:568	Although glucose and other sugars activate the T1R2 + T1R3 "sweet" taste receptor, recent evidence from T1R2- or T1R3-knockout (KO) mice suggests that maltodextrins, despite their glucose polymer composition, activate a separate receptor mechanism to generate a taste percept qualitatively distinguishable from that of sweeteners.
28768658	6	63	theme	allelic	1189:1195	arg1	variation					1197:1205	potential allelic variation	1179:1205	potential allelic variation in the putative polysaccharide receptor	1179:1245	However, some KO mice were only sensitive to the higher Polycose concentrations, implicating potential allelic variation in the putative polysaccharide receptor or downstream pathways unmasked by the absence of T1R2 + T1R3.
28768658	6	64	from	variation	1197:1205	arg1	receptor					1238:1245	the putative polysaccharide receptor	1210:1245	the putative polysaccharide receptor	1210:1245	However, some KO mice were only sensitive to the higher Polycose concentrations, implicating potential allelic variation in the putative polysaccharide receptor or downstream pathways unmasked by the absence of T1R2 + T1R3.
28768658	8	65	from	percepts	1644:1651	arg1	mice					1755:1758	KO mice	1752:1758	KO mice	1752:1758	Although both genotypes competently discriminated Maltrin from sucrose, performance was apparently driven by the different orosensory percepts of the two stimuli in control mice and the presence of a Maltrin but not sucrose orosensory cue in KO mice.
28768658	8	65	from	percepts	1644:1651	arg1	mice					1683:1686	control mice	1675:1686	control mice	1675:1686	Although both genotypes competently discriminated Maltrin from sucrose, performance was apparently driven by the different orosensory percepts of the two stimuli in control mice and the presence of a Maltrin but not sucrose orosensory cue in KO mice.
28768658	3	66	theme	explicit	580:587	arg1	discrimination					589:602	explicit discrimination	580:602	explicit discrimination of maltodextrins from prototypical sweeteners	580:648	However, explicit discrimination of maltodextrins from prototypical sweeteners has not yet been psychophysically tested in any murine model.
28768658	2	67	dep	T1R2	287:290	arg1	the					283:285	the	283:285	the	283:285	Although glucose and other sugars activate the T1R2 + T1R3 "sweet" taste receptor, recent evidence from T1R2- or T1R3-knockout (KO) mice suggests that maltodextrins, despite their glucose polymer composition, activate a separate receptor mechanism to generate a taste percept qualitatively distinguishable from that of sweeteners.
28768658	5	68	theme	KO	1026:1027	arg1	mice					1029:1032	Most KO mice	1021:1032	Most KO mice	1021:1032	Most KO mice displayed similar Polycose sensitivity as controls.
28768658	6	69	from	pathways	1261:1268	arg1	receptor					1238:1245	the putative polysaccharide receptor	1210:1245	the putative polysaccharide receptor	1210:1245	However, some KO mice were only sensitive to the higher Polycose concentrations, implicating potential allelic variation in the putative polysaccharide receptor or downstream pathways unmasked by the absence of T1R2 + T1R3.
28768658	2	70	theme	polymer	428:434	arg1	composition					436:446	their glucose polymer composition	414:446	their glucose polymer composition	414:446	Although glucose and other sugars activate the T1R2 + T1R3 "sweet" taste receptor, recent evidence from T1R2- or T1R3-knockout (KO) mice suggests that maltodextrins, despite their glucose polymer composition, activate a separate receptor mechanism to generate a taste percept qualitatively distinguishable from that of sweeteners.
28768658	2	71	theme	receptor	469:476	arg1	mechanism					478:486	a separate receptor mechanism	458:486	a separate receptor mechanism to generate a taste percept qualitatively distinguishable from that of sweeteners	458:568	Although glucose and other sugars activate the T1R2 + T1R3 "sweet" taste receptor, recent evidence from T1R2- or T1R3-knockout (KO) mice suggests that maltodextrins, despite their glucose polymer composition, activate a separate receptor mechanism to generate a taste percept qualitatively distinguishable from that of sweeteners.
28768658	0	72	from	sucrose	54:60	arg1	discrimination					34:47	its discrimination	30:47	its discrimination from sucrose	30:60	Detection of maltodextrin and its discrimination from sucrose are independent of the T1R2 + T1R3 heterodimer.
28768658	0	72	from	sucrose	54:60	arg1	Detection					0:8	Detection	0:8	Detection of maltodextrin	0:24	Detection of maltodextrin and its discrimination from sucrose are independent of the T1R2 + T1R3 heterodimer.
28768658	0	73	theme	heterodimer	97:107	arg1	independent					66:76	independent	66:76	independent	66:76	Detection of maltodextrin and its discrimination from sucrose are independent of the T1R2 + T1R3 heterodimer.
28768658	2	74	theme	T1R2-	344:348	arg1	mice					372:375	T1R2- or T1R3-knockout (KO) mice	344:375	T1R2- or T1R3-knockout (KO) mice	344:375	Although glucose and other sugars activate the T1R2 + T1R3 "sweet" taste receptor, recent evidence from T1R2- or T1R3-knockout (KO) mice suggests that maltodextrins, despite their glucose polymer composition, activate a separate receptor mechanism to generate a taste percept qualitatively distinguishable from that of sweeteners.
28768658	8	75	from	mice	1683:1686	arg1	percepts					1644:1651	the different orosensory percepts	1619:1651	the different orosensory percepts of the two stimuli in control mice	1619:1686	Although both genotypes competently discriminated Maltrin from sucrose, performance was apparently driven by the different orosensory percepts of the two stimuli in control mice and the presence of a Maltrin but not sucrose orosensory cue in KO mice.
28768658	8	75	from	mice	1683:1686	arg1	presence					1696:1703	the presence	1692:1703	the presence of a Maltrin but not sucrose orosensory cue in KO mice	1692:1758	Although both genotypes competently discriminated Maltrin from sucrose, performance was apparently driven by the different orosensory percepts of the two stimuli in control mice and the presence of a Maltrin but not sucrose orosensory cue in KO mice.
28768658	2	76	theme	KO	368:369	arg1	mice					372:375	T1R2- or T1R3-knockout (KO) mice	344:375	T1R2- or T1R3-knockout (KO) mice	344:375	Although glucose and other sugars activate the T1R2 + T1R3 "sweet" taste receptor, recent evidence from T1R2- or T1R3-knockout (KO) mice suggests that maltodextrins, despite their glucose polymer composition, activate a separate receptor mechanism to generate a taste percept qualitatively distinguishable from that of sweeteners.
28768658	9	77	theme	receptor	1819:1826	arg1	mechanism					1828:1836	an orosensory receptor mechanism	1805:1836	an orosensory receptor mechanism that gives rise to a qualitatively distinguishable sensation from that of sucrose	1805:1918	These data support the proposed presence of an orosensory receptor mechanism that gives rise to a qualitatively distinguishable sensation from that of sucrose.
28768658	2	78	theme	T1R3-knockout	353:365	arg1	mice					372:375	T1R2- or T1R3-knockout (KO) mice	344:375	T1R2- or T1R3-knockout (KO) mice	344:375	Although glucose and other sugars activate the T1R2 + T1R3 "sweet" taste receptor, recent evidence from T1R2- or T1R3-knockout (KO) mice suggests that maltodextrins, despite their glucose polymer composition, activate a separate receptor mechanism to generate a taste percept qualitatively distinguishable from that of sweeteners.
28768658	6	79	theme	KO	1100:1101	arg1	mice					1103:1106	some KO mice	1095:1106	some KO mice	1095:1106	However, some KO mice were only sensitive to the higher Polycose concentrations, implicating potential allelic variation in the putative polysaccharide receptor or downstream pathways unmasked by the absence of T1R2 + T1R3.
28768658	4	80	theme	two-response	788:799	arg1	task					822:825	a two-response taste discrimination task	786:825	a two-response taste discrimination task	786:825	Therefore, mice lacking T1R2 + T1R3 and wild-type controls were tested in a two-response taste discrimination task to determine whether maltodextrins are 1) detectable when both receptor subunits are absent and 2) perceptually distinct from that of sucrose irrespective of viscosity, intensity, and hedonics.
28768658	2	81	theme	sweet	300:304	arg1	"					305:305	T1R3 "sweet"	294:305	T1R3 "sweet"	294:305	Although glucose and other sugars activate the T1R2 + T1R3 "sweet" taste receptor, recent evidence from T1R2- or T1R3-knockout (KO) mice suggests that maltodextrins, despite their glucose polymer composition, activate a separate receptor mechanism to generate a taste percept qualitatively distinguishable from that of sweeteners.
28768658	8	82	theme	stimuli	1664:1670	arg1	percepts					1644:1651	the different orosensory percepts	1619:1651	the different orosensory percepts of the two stimuli in control mice	1619:1686	Although both genotypes competently discriminated Maltrin from sucrose, performance was apparently driven by the different orosensory percepts of the two stimuli in control mice and the presence of a Maltrin but not sucrose orosensory cue in KO mice.
28768658	8	82	theme	stimuli	1664:1670	arg1	presence					1696:1703	the presence	1692:1703	the presence of a Maltrin but not sucrose orosensory cue in KO mice	1692:1758	Although both genotypes competently discriminated Maltrin from sucrose, performance was apparently driven by the different orosensory percepts of the two stimuli in control mice and the presence of a Maltrin but not sucrose orosensory cue in KO mice.
28768658	4	83	theme	wild-type	752:760	arg1	controls					762:769	wild-type controls	752:769	wild-type controls	752:769	Therefore, mice lacking T1R2 + T1R3 and wild-type controls were tested in a two-response taste discrimination task to determine whether maltodextrins are 1) detectable when both receptor subunits are absent and 2) perceptually distinct from that of sucrose irrespective of viscosity, intensity, and hedonics.
28768658	2	84	theme	T1R3	294:297	arg1	"					305:305	T1R3 "sweet"	294:305	T1R3 "sweet"	294:305	Although glucose and other sugars activate the T1R2 + T1R3 "sweet" taste receptor, recent evidence from T1R2- or T1R3-knockout (KO) mice suggests that maltodextrins, despite their glucose polymer composition, activate a separate receptor mechanism to generate a taste percept qualitatively distinguishable from that of sweeteners.
28768658	8	85	theme	control	1675:1681	arg1	mice					1683:1686	control mice	1675:1686	control mice	1675:1686	Although both genotypes competently discriminated Maltrin from sucrose, performance was apparently driven by the different orosensory percepts of the two stimuli in control mice and the presence of a Maltrin but not sucrose orosensory cue in KO mice.
28768658	4	86	from	that	953:956	arg1	distinct					939:946	distinct	939:946	distinct	939:946	Therefore, mice lacking T1R2 + T1R3 and wild-type controls were tested in a two-response taste discrimination task to determine whether maltodextrins are 1) detectable when both receptor subunits are absent and 2) perceptually distinct from that of sucrose irrespective of viscosity, intensity, and hedonics.
28768658	7	87	theme	solutions	1488:1496	arg1	intensity					1452:1460	intensity	1452:1460	intensity	1452:1460	Varied Maltrin and sucrose concentrations of approximately matched viscosities were then presented to render the oral somatosensory features, intensity, and hedonic value of the solutions irrelevant.
28768658	7	87	theme	solutions	1488:1496	arg1	features					1442:1449	the oral somatosensory features	1419:1449	the oral somatosensory features	1419:1449	Varied Maltrin and sucrose concentrations of approximately matched viscosities were then presented to render the oral somatosensory features, intensity, and hedonic value of the solutions irrelevant.
28768658	7	87	theme	solutions	1488:1496	arg1	value					1475:1479	hedonic value	1467:1479	hedonic value of the solutions irrelevant	1467:1507	Varied Maltrin and sucrose concentrations of approximately matched viscosities were then presented to render the oral somatosensory features, intensity, and hedonic value of the solutions irrelevant.
28768658	8	88	theme	different	1623:1631	arg1	percepts					1644:1651	the different orosensory percepts	1619:1651	the different orosensory percepts of the two stimuli in control mice	1619:1686	Although both genotypes competently discriminated Maltrin from sucrose, performance was apparently driven by the different orosensory percepts of the two stimuli in control mice and the presence of a Maltrin but not sucrose orosensory cue in KO mice.
28768658	9	89	theme	proposed	1784:1791	arg1	presence					1793:1800	the proposed presence	1780:1800	the proposed presence of an orosensory receptor mechanism that gives rise to a qualitatively distinguishable sensation from that of sucrose	1780:1918	These data support the proposed presence of an orosensory receptor mechanism that gives rise to a qualitatively distinguishable sensation from that of sucrose.
27246376	5	0	theme	monosaccharide	1039:1052	arg1	compositions					1054:1065	their monosaccharide compositions	1033:1065	their monosaccharide compositions	1033:1065	Subsequently, the crude LEPs were further purified by DEAE-cellulose and Sephadex-100 chromatography to obtain two fractions, which were designated as LEP-1 and LEP-2 and their monosaccharide compositions were characterized by GC.
27246376	0	1	from	purification	12:23	arg1	Lentinus					83:90	Lentinus	83:90	Lentinus	83:90	Extraction, purification and anti-proliferative activities of polysaccharides from Lentinus edodes.
27246376	1	2	theme	Lentinus	170:177	arg1	LEPs					187:190	LEPs	187:190	LEPs	187:190	In this study, the enzyme-assisted extraction of polysaccharides from Lentinus edodes (LEPs) was optimized by response surface methodology, and a preliminary characterization of the extracted LEPs and their anti-proliferative activities were investigated.
27246376	1	2	theme	Lentinus	170:177	arg1	edodes					179:184	Lentinus edodes	170:184	Lentinus edodes (LEPs)	170:191	In this study, the enzyme-assisted extraction of polysaccharides from Lentinus edodes (LEPs) was optimized by response surface methodology, and a preliminary characterization of the extracted LEPs and their anti-proliferative activities were investigated.
27246376	1	3	theme	preliminary	246:256	arg1	characterization					258:273	a preliminary characterization	244:273	a preliminary characterization of the extracted LEPs and their anti-proliferative activities	244:335	In this study, the enzyme-assisted extraction of polysaccharides from Lentinus edodes (LEPs) was optimized by response surface methodology, and a preliminary characterization of the extracted LEPs and their anti-proliferative activities were investigated.
27246376	8	4	theme	food	1495:1498	arg1	industries					1500:1509	the pharmaceutical and functional food industries	1461:1509	the pharmaceutical and functional food industries	1461:1509	In summary, this study provides an efficient enzyme-assisted extraction for LEPs, which can be used as natural antitumor agents in the pharmaceutical and functional food industries.
27246376	4	5	theme	%	859:859	arg1	yield					845:849	the predicted yield	831:849	the predicted yield of 15.58%	831:859	The results showed that the highest polysaccharides yield of 15.65% was achieved with an extraction temperature of 54°C, pH 5.0, enzymatic treatment time of 93min and a liquid/material ratio of 29:1mL/g, which correlated well with the predicted yield of 15.58%.
27246376	8	6	theme	enzyme-assisted	1375:1389	arg1	agents					1451:1456	natural antitumor agents	1433:1456	natural antitumor agents in the pharmaceutical and functional food industries	1433:1509	In summary, this study provides an efficient enzyme-assisted extraction for LEPs, which can be used as natural antitumor agents in the pharmaceutical and functional food industries.
27246376	8	6	theme	enzyme-assisted	1375:1389	arg1	extraction					1391:1400	an efficient enzyme-assisted extraction	1362:1400	an efficient enzyme-assisted extraction	1362:1400	In summary, this study provides an efficient enzyme-assisted extraction for LEPs, which can be used as natural antitumor agents in the pharmaceutical and functional food industries.
27246376	0	7	from	Extraction	0:9	arg1	Lentinus					83:90	Lentinus	83:90	Lentinus	83:90	Extraction, purification and anti-proliferative activities of polysaccharides from Lentinus edodes.
27246376	1	8	from	edodes	179:184	arg1	extraction					135:144	the enzyme-assisted extraction	115:144	the enzyme-assisted extraction of polysaccharides from Lentinus edodes (LEPs)	115:191	In this study, the enzyme-assisted extraction of polysaccharides from Lentinus edodes (LEPs) was optimized by response surface methodology, and a preliminary characterization of the extracted LEPs and their anti-proliferative activities were investigated.
27246376	1	8	from	edodes	179:184	arg1	polysaccharides					149:163	polysaccharides	149:163	polysaccharides from Lentinus edodes (LEPs)	149:191	In this study, the enzyme-assisted extraction of polysaccharides from Lentinus edodes (LEPs) was optimized by response surface methodology, and a preliminary characterization of the extracted LEPs and their anti-proliferative activities were investigated.
27246376	4	9	theme	93min	757:761	arg1	temperature					700:710	an extraction temperature	686:710	an extraction temperature of 54°C	686:718	The results showed that the highest polysaccharides yield of 15.65% was achieved with an extraction temperature of 54°C, pH 5.0, enzymatic treatment time of 93min and a liquid/material ratio of 29:1mL/g, which correlated well with the predicted yield of 15.58%.
27246376	4	9	theme	93min	757:761	arg1	time					749:752	enzymatic treatment time	729:752	enzymatic treatment time of 93min	729:761	The results showed that the highest polysaccharides yield of 15.65% was achieved with an extraction temperature of 54°C, pH 5.0, enzymatic treatment time of 93min and a liquid/material ratio of 29:1mL/g, which correlated well with the predicted yield of 15.58%.
27246376	4	9	theme	93min	757:761	arg1	pH					721:722	pH 5.0	721:726	pH 5.0	721:726	The results showed that the highest polysaccharides yield of 15.65% was achieved with an extraction temperature of 54°C, pH 5.0, enzymatic treatment time of 93min and a liquid/material ratio of 29:1mL/g, which correlated well with the predicted yield of 15.58%.
27246376	4	9	theme	93min	757:761	arg1	ratio					785:789	a liquid/material ratio	767:789	a liquid/material ratio of 29:1mL/g, which correlated well with the predicted yield of 15.58%	767:859	The results showed that the highest polysaccharides yield of 15.65% was achieved with an extraction temperature of 54°C, pH 5.0, enzymatic treatment time of 93min and a liquid/material ratio of 29:1mL/g, which correlated well with the predicted yield of 15.58%.
27246376	5	10	theme	Sephadex-100	935:946	arg1	chromatography					948:961	DEAE-cellulose and Sephadex-100 chromatography	916:961	chromatography	948:961	Subsequently, the crude LEPs were further purified by DEAE-cellulose and Sephadex-100 chromatography to obtain two fractions, which were designated as LEP-1 and LEP-2 and their monosaccharide compositions were characterized by GC.
27246376	8	11	theme	efficient	1365:1373	arg1	agents					1451:1456	natural antitumor agents	1433:1456	natural antitumor agents in the pharmaceutical and functional food industries	1433:1509	In summary, this study provides an efficient enzyme-assisted extraction for LEPs, which can be used as natural antitumor agents in the pharmaceutical and functional food industries.
27246376	8	11	theme	efficient	1365:1373	arg1	extraction					1391:1400	an efficient enzyme-assisted extraction	1362:1400	an efficient enzyme-assisted extraction	1362:1400	In summary, this study provides an efficient enzyme-assisted extraction for LEPs, which can be used as natural antitumor agents in the pharmaceutical and functional food industries.
27246376	4	12	theme	extraction	689:698	arg1	temperature					700:710	an extraction temperature	686:710	an extraction temperature of 54°C	686:718	The results showed that the highest polysaccharides yield of 15.65% was achieved with an extraction temperature of 54°C, pH 5.0, enzymatic treatment time of 93min and a liquid/material ratio of 29:1mL/g, which correlated well with the predicted yield of 15.58%.
27246376	7	13	theme	proliferation	1280:1292	arg1	inhibition					1261:1270	inhibition	1261:1270	inhibition of cell proliferation	1261:1292	In addition, the LEPs exhibited inhibition of cell proliferation on HCT-116 and HeLa cells in vitro.
27246376	4	14	theme	polysaccharides	636:650	arg1	yield					652:656	the highest polysaccharides yield	624:656	the highest polysaccharides yield of 15.65%	624:666	The results showed that the highest polysaccharides yield of 15.65% was achieved with an extraction temperature of 54°C, pH 5.0, enzymatic treatment time of 93min and a liquid/material ratio of 29:1mL/g, which correlated well with the predicted yield of 15.58%.
27246376	3	15	theme	conditions	532:541	arg1	effects					510:516	effects	510:516	effects of extraction conditions	510:541	Then effects of extraction conditions were evaluated and optimized using a Box-Behnken design.
27246376	0	16	from	activities	48:57	arg1	Lentinus					83:90	Lentinus	83:90	Lentinus	83:90	Extraction, purification and anti-proliferative activities of polysaccharides from Lentinus edodes.
27246376	1	17	from	extraction	135:144	arg1	LEPs					187:190	LEPs	187:190	LEPs	187:190	In this study, the enzyme-assisted extraction of polysaccharides from Lentinus edodes (LEPs) was optimized by response surface methodology, and a preliminary characterization of the extracted LEPs and their anti-proliferative activities were investigated.
27246376	1	17	from	extraction	135:144	arg1	edodes					179:184	Lentinus edodes	170:184	Lentinus edodes (LEPs)	170:191	In this study, the enzyme-assisted extraction of polysaccharides from Lentinus edodes (LEPs) was optimized by response surface methodology, and a preliminary characterization of the extracted LEPs and their anti-proliferative activities were investigated.
27246376	4	18	theme	%	666:666	arg1	yield					652:656	the highest polysaccharides yield	624:656	the highest polysaccharides yield of 15.65%	624:666	The results showed that the highest polysaccharides yield of 15.65% was achieved with an extraction temperature of 54°C, pH 5.0, enzymatic treatment time of 93min and a liquid/material ratio of 29:1mL/g, which correlated well with the predicted yield of 15.58%.
27246376	4	19	theme	enzymatic	729:737	arg1	time					749:752	enzymatic treatment time	729:752	enzymatic treatment time of 93min	729:761	The results showed that the highest polysaccharides yield of 15.65% was achieved with an extraction temperature of 54°C, pH 5.0, enzymatic treatment time of 93min and a liquid/material ratio of 29:1mL/g, which correlated well with the predicted yield of 15.58%.
27246376	6	20	from	other	1186:1190	arg1	distinct					1167:1174	distinct	1167:1174	distinct	1167:1174	Fourier-transform infrared spectra demonstrated that LEP-1 and LEP-2 were distinct from each other regarding their chemical structures.
27246376	2	21	theme	optimal	409:415	arg1	amounts					417:423	the optimal amounts	405:423	the optimal amounts of cellulase, papain and pectinase, which were 15, 20 and 15g/kg, respectively	405:502	An orthogonal assay was constructed to determine the optimal amounts of cellulase, papain and pectinase, which were 15, 20 and 15g/kg, respectively.
27246376	2	21	theme	optimal	409:415	arg1	pectinase					450:458	pectinase	450:458	pectinase	450:458	An orthogonal assay was constructed to determine the optimal amounts of cellulase, papain and pectinase, which were 15, 20 and 15g/kg, respectively.
27246376	2	21	theme	optimal	409:415	arg1	papain					439:444	papain	439:444	papain	439:444	An orthogonal assay was constructed to determine the optimal amounts of cellulase, papain and pectinase, which were 15, 20 and 15g/kg, respectively.
27246376	2	21	theme	optimal	409:415	arg1	cellulase					428:436	cellulase	428:436	cellulase	428:436	An orthogonal assay was constructed to determine the optimal amounts of cellulase, papain and pectinase, which were 15, 20 and 15g/kg, respectively.
27246376	6	22	theme	chemical	1208:1215	arg1	structures					1217:1226	their chemical structures	1202:1226	their chemical structures	1202:1226	Fourier-transform infrared spectra demonstrated that LEP-1 and LEP-2 were distinct from each other regarding their chemical structures.
27246376	1	23	theme	extracted	282:290	arg1	LEPs					292:295	the extracted LEPs	278:295	the extracted LEPs	278:295	In this study, the enzyme-assisted extraction of polysaccharides from Lentinus edodes (LEPs) was optimized by response surface methodology, and a preliminary characterization of the extracted LEPs and their anti-proliferative activities were investigated.
27246376	4	24	theme	predicted	835:843	arg1	yield					845:849	the predicted yield	831:849	the predicted yield of 15.58%	831:859	The results showed that the highest polysaccharides yield of 15.65% was achieved with an extraction temperature of 54°C, pH 5.0, enzymatic treatment time of 93min and a liquid/material ratio of 29:1mL/g, which correlated well with the predicted yield of 15.58%.
27246376	3	25	theme	Box-Behnken	580:590	arg1	design					592:597	a Box-Behnken design	578:597	a Box-Behnken design	578:597	Then effects of extraction conditions were evaluated and optimized using a Box-Behnken design.
27246376	5	26	theme	crude	880:884	arg1	LEPs					886:889	the crude LEPs	876:889	the crude LEPs	876:889	Subsequently, the crude LEPs were further purified by DEAE-cellulose and Sephadex-100 chromatography to obtain two fractions, which were designated as LEP-1 and LEP-2 and their monosaccharide compositions were characterized by GC.
27246376	1	27	theme	LEPs	292:295	arg1	characterization					258:273	a preliminary characterization	244:273	a preliminary characterization of the extracted LEPs and their anti-proliferative activities	244:335	In this study, the enzyme-assisted extraction of polysaccharides from Lentinus edodes (LEPs) was optimized by response surface methodology, and a preliminary characterization of the extracted LEPs and their anti-proliferative activities were investigated.
27246376	2	28	theme	pectinase	450:458	arg1	amounts					417:423	the optimal amounts	405:423	the optimal amounts of cellulase, papain and pectinase, which were 15, 20 and 15g/kg, respectively	405:502	An orthogonal assay was constructed to determine the optimal amounts of cellulase, papain and pectinase, which were 15, 20 and 15g/kg, respectively.
27246376	2	28	theme	pectinase	450:458	arg1	pectinase					450:458	pectinase	450:458	pectinase	450:458	An orthogonal assay was constructed to determine the optimal amounts of cellulase, papain and pectinase, which were 15, 20 and 15g/kg, respectively.
27246376	2	28	theme	pectinase	450:458	arg1	papain					439:444	papain	439:444	papain	439:444	An orthogonal assay was constructed to determine the optimal amounts of cellulase, papain and pectinase, which were 15, 20 and 15g/kg, respectively.
27246376	2	28	theme	pectinase	450:458	arg1	cellulase					428:436	cellulase	428:436	cellulase	428:436	An orthogonal assay was constructed to determine the optimal amounts of cellulase, papain and pectinase, which were 15, 20 and 15g/kg, respectively.
27246376	5	29	theme	DEAE-cellulose	916:929	arg1	chromatography					948:961	DEAE-cellulose and Sephadex-100 chromatography	916:961	chromatography	948:961	Subsequently, the crude LEPs were further purified by DEAE-cellulose and Sephadex-100 chromatography to obtain two fractions, which were designated as LEP-1 and LEP-2 and their monosaccharide compositions were characterized by GC.
27246376	8	30	theme	antitumor	1441:1449	arg1	agents					1451:1456	natural antitumor agents	1433:1456	natural antitumor agents in the pharmaceutical and functional food industries	1433:1509	In summary, this study provides an efficient enzyme-assisted extraction for LEPs, which can be used as natural antitumor agents in the pharmaceutical and functional food industries.
27246376	8	30	theme	antitumor	1441:1449	arg1	extraction					1391:1400	an efficient enzyme-assisted extraction	1362:1400	an efficient enzyme-assisted extraction	1362:1400	In summary, this study provides an efficient enzyme-assisted extraction for LEPs, which can be used as natural antitumor agents in the pharmaceutical and functional food industries.
27246376	4	31	theme	treatment	739:747	arg1	time					749:752	enzymatic treatment time	729:752	enzymatic treatment time of 93min	729:761	The results showed that the highest polysaccharides yield of 15.65% was achieved with an extraction temperature of 54°C, pH 5.0, enzymatic treatment time of 93min and a liquid/material ratio of 29:1mL/g, which correlated well with the predicted yield of 15.58%.
27246376	2	32	theme	papain	439:444	arg1	amounts					417:423	the optimal amounts	405:423	the optimal amounts of cellulase, papain and pectinase, which were 15, 20 and 15g/kg, respectively	405:502	An orthogonal assay was constructed to determine the optimal amounts of cellulase, papain and pectinase, which were 15, 20 and 15g/kg, respectively.
27246376	2	32	theme	papain	439:444	arg1	pectinase					450:458	pectinase	450:458	pectinase	450:458	An orthogonal assay was constructed to determine the optimal amounts of cellulase, papain and pectinase, which were 15, 20 and 15g/kg, respectively.
27246376	2	32	theme	papain	439:444	arg1	papain					439:444	papain	439:444	papain	439:444	An orthogonal assay was constructed to determine the optimal amounts of cellulase, papain and pectinase, which were 15, 20 and 15g/kg, respectively.
27246376	2	32	theme	papain	439:444	arg1	cellulase					428:436	cellulase	428:436	cellulase	428:436	An orthogonal assay was constructed to determine the optimal amounts of cellulase, papain and pectinase, which were 15, 20 and 15g/kg, respectively.
27246376	7	33	theme	HeLa	1309:1312	arg1	cells					1314:1318	HCT-116 and HeLa cells	1297:1318	cells	1314:1318	In addition, the LEPs exhibited inhibition of cell proliferation on HCT-116 and HeLa cells in vitro.
27246376	2	34	theme	orthogonal	359:368	arg1	assay					370:374	An orthogonal assay	356:374	An orthogonal assay	356:374	An orthogonal assay was constructed to determine the optimal amounts of cellulase, papain and pectinase, which were 15, 20 and 15g/kg, respectively.
27246376	8	35	theme	natural	1433:1439	arg1	agents					1451:1456	natural antitumor agents	1433:1456	natural antitumor agents in the pharmaceutical and functional food industries	1433:1509	In summary, this study provides an efficient enzyme-assisted extraction for LEPs, which can be used as natural antitumor agents in the pharmaceutical and functional food industries.
27246376	8	35	theme	natural	1433:1439	arg1	extraction					1391:1400	an efficient enzyme-assisted extraction	1362:1400	an efficient enzyme-assisted extraction	1362:1400	In summary, this study provides an efficient enzyme-assisted extraction for LEPs, which can be used as natural antitumor agents in the pharmaceutical and functional food industries.
27246376	1	36	theme	response	210:217	arg1	methodology					227:237	response surface methodology	210:237	response surface methodology	210:237	In this study, the enzyme-assisted extraction of polysaccharides from Lentinus edodes (LEPs) was optimized by response surface methodology, and a preliminary characterization of the extracted LEPs and their anti-proliferative activities were investigated.
27246376	0	37	theme	anti-proliferative	29:46	arg1	activities					48:57	anti-proliferative activities	29:57	anti-proliferative activities	29:57	Extraction, purification and anti-proliferative activities of polysaccharides from Lentinus edodes.
27246376	1	38	theme	anti-proliferative	307:324	arg1	activities					326:335	their anti-proliferative activities	301:335	their anti-proliferative activities	301:335	In this study, the enzyme-assisted extraction of polysaccharides from Lentinus edodes (LEPs) was optimized by response surface methodology, and a preliminary characterization of the extracted LEPs and their anti-proliferative activities were investigated.
27246376	0	39	from	Lentinus	83:90	arg1	purification					12:23	purification	12:23	purification	12:23	Extraction, purification and anti-proliferative activities of polysaccharides from Lentinus edodes.
27246376	0	39	from	Lentinus	83:90	arg1	activities					48:57	anti-proliferative activities	29:57	anti-proliferative activities	29:57	Extraction, purification and anti-proliferative activities of polysaccharides from Lentinus edodes.
27246376	0	39	from	Lentinus	83:90	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction, purification and anti-proliferative activities of polysaccharides from Lentinus edodes.
27246376	0	39	from	Lentinus	83:90	arg1	polysaccharides					62:76	polysaccharides	62:76	polysaccharides from Lentinus	62:90	Extraction, purification and anti-proliferative activities of polysaccharides from Lentinus edodes.
27246376	1	40	theme	surface	219:225	arg1	methodology					227:237	response surface methodology	210:237	response surface methodology	210:237	In this study, the enzyme-assisted extraction of polysaccharides from Lentinus edodes (LEPs) was optimized by response surface methodology, and a preliminary characterization of the extracted LEPs and their anti-proliferative activities were investigated.
27246376	0	41	theme	polysaccharides	62:76	arg1	purification					12:23	purification	12:23	purification	12:23	Extraction, purification and anti-proliferative activities of polysaccharides from Lentinus edodes.
27246376	0	41	theme	polysaccharides	62:76	arg1	activities					48:57	anti-proliferative activities	29:57	anti-proliferative activities	29:57	Extraction, purification and anti-proliferative activities of polysaccharides from Lentinus edodes.
27246376	0	41	theme	polysaccharides	62:76	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction, purification and anti-proliferative activities of polysaccharides from Lentinus edodes.
27246376	1	42	theme	activities	326:335	arg1	characterization					258:273	a preliminary characterization	244:273	a preliminary characterization of the extracted LEPs and their anti-proliferative activities	244:335	In this study, the enzyme-assisted extraction of polysaccharides from Lentinus edodes (LEPs) was optimized by response surface methodology, and a preliminary characterization of the extracted LEPs and their anti-proliferative activities were investigated.
27246376	6	43	theme	infrared	1111:1118	arg1	spectra					1120:1126	Fourier-transform infrared spectra	1093:1126	Fourier-transform infrared spectra	1093:1126	Fourier-transform infrared spectra demonstrated that LEP-1 and LEP-2 were distinct from each other regarding their chemical structures.
27246376	8	44	theme	pharmaceutical	1465:1478	arg1	industries					1500:1509	the pharmaceutical and functional food industries	1461:1509	the pharmaceutical and functional food industries	1461:1509	In summary, this study provides an efficient enzyme-assisted extraction for LEPs, which can be used as natural antitumor agents in the pharmaceutical and functional food industries.
27246376	1	45	theme	enzyme-assisted	119:133	arg1	extraction					135:144	the enzyme-assisted extraction	115:144	the enzyme-assisted extraction of polysaccharides from Lentinus edodes (LEPs)	115:191	In this study, the enzyme-assisted extraction of polysaccharides from Lentinus edodes (LEPs) was optimized by response surface methodology, and a preliminary characterization of the extracted LEPs and their anti-proliferative activities were investigated.
27246376	7	46	theme	cell	1275:1278	arg1	proliferation					1280:1292	cell proliferation	1275:1292	cell proliferation	1275:1292	In addition, the LEPs exhibited inhibition of cell proliferation on HCT-116 and HeLa cells in vitro.
27246376	8	47	theme	functional	1484:1493	arg1	industries					1500:1509	the pharmaceutical and functional food industries	1461:1509	the pharmaceutical and functional food industries	1461:1509	In summary, this study provides an efficient enzyme-assisted extraction for LEPs, which can be used as natural antitumor agents in the pharmaceutical and functional food industries.
27246376	4	48	theme	liquid/material	769:783	arg1	ratio					785:789	a liquid/material ratio	767:789	a liquid/material ratio of 29:1mL/g, which correlated well with the predicted yield of 15.58%	767:859	The results showed that the highest polysaccharides yield of 15.65% was achieved with an extraction temperature of 54°C, pH 5.0, enzymatic treatment time of 93min and a liquid/material ratio of 29:1mL/g, which correlated well with the predicted yield of 15.58%.
27246376	6	49	theme	Fourier-transform	1093:1109	arg1	spectra					1120:1126	Fourier-transform infrared spectra	1093:1126	Fourier-transform infrared spectra	1093:1126	Fourier-transform infrared spectra demonstrated that LEP-1 and LEP-2 were distinct from each other regarding their chemical structures.
27246376	8	50	used	used	1425:1428	arg2	extraction					1391:1400	an efficient enzyme-assisted extraction	1362:1400	an efficient enzyme-assisted extraction	1362:1400	In summary, this study provides an efficient enzyme-assisted extraction for LEPs, which can be used as natural antitumor agents in the pharmaceutical and functional food industries.
27246376	8	50	used	used	1425:1428	arg2	agents					1451:1456	natural antitumor agents	1433:1456	natural antitumor agents in the pharmaceutical and functional food industries	1433:1509	In summary, this study provides an efficient enzyme-assisted extraction for LEPs, which can be used as natural antitumor agents in the pharmaceutical and functional food industries.
27246376	2	51	theme	cellulase	428:436	arg1	amounts					417:423	the optimal amounts	405:423	the optimal amounts of cellulase, papain and pectinase, which were 15, 20 and 15g/kg, respectively	405:502	An orthogonal assay was constructed to determine the optimal amounts of cellulase, papain and pectinase, which were 15, 20 and 15g/kg, respectively.
27246376	2	51	theme	cellulase	428:436	arg1	pectinase					450:458	pectinase	450:458	pectinase	450:458	An orthogonal assay was constructed to determine the optimal amounts of cellulase, papain and pectinase, which were 15, 20 and 15g/kg, respectively.
27246376	2	51	theme	cellulase	428:436	arg1	papain					439:444	papain	439:444	papain	439:444	An orthogonal assay was constructed to determine the optimal amounts of cellulase, papain and pectinase, which were 15, 20 and 15g/kg, respectively.
27246376	2	51	theme	cellulase	428:436	arg1	cellulase					428:436	cellulase	428:436	cellulase	428:436	An orthogonal assay was constructed to determine the optimal amounts of cellulase, papain and pectinase, which were 15, 20 and 15g/kg, respectively.
27246376	4	52	theme	29:1mL/g	794:801	arg1	temperature					700:710	an extraction temperature	686:710	an extraction temperature of 54°C	686:718	The results showed that the highest polysaccharides yield of 15.65% was achieved with an extraction temperature of 54°C, pH 5.0, enzymatic treatment time of 93min and a liquid/material ratio of 29:1mL/g, which correlated well with the predicted yield of 15.58%.
27246376	4	52	theme	29:1mL/g	794:801	arg1	time					749:752	enzymatic treatment time	729:752	enzymatic treatment time of 93min	729:761	The results showed that the highest polysaccharides yield of 15.65% was achieved with an extraction temperature of 54°C, pH 5.0, enzymatic treatment time of 93min and a liquid/material ratio of 29:1mL/g, which correlated well with the predicted yield of 15.58%.
27246376	4	52	theme	29:1mL/g	794:801	arg1	pH					721:722	pH 5.0	721:726	pH 5.0	721:726	The results showed that the highest polysaccharides yield of 15.65% was achieved with an extraction temperature of 54°C, pH 5.0, enzymatic treatment time of 93min and a liquid/material ratio of 29:1mL/g, which correlated well with the predicted yield of 15.58%.
27246376	4	52	theme	29:1mL/g	794:801	arg1	ratio					785:789	a liquid/material ratio	767:789	a liquid/material ratio of 29:1mL/g, which correlated well with the predicted yield of 15.58%	767:859	The results showed that the highest polysaccharides yield of 15.65% was achieved with an extraction temperature of 54°C, pH 5.0, enzymatic treatment time of 93min and a liquid/material ratio of 29:1mL/g, which correlated well with the predicted yield of 15.58%.
27246376	1	53	theme	polysaccharides	149:163	arg1	extraction					135:144	the enzyme-assisted extraction	115:144	the enzyme-assisted extraction of polysaccharides from Lentinus edodes (LEPs)	115:191	In this study, the enzyme-assisted extraction of polysaccharides from Lentinus edodes (LEPs) was optimized by response surface methodology, and a preliminary characterization of the extracted LEPs and their anti-proliferative activities were investigated.
27246376	4	54	theme	highest	628:634	arg1	yield					652:656	the highest polysaccharides yield	624:656	the highest polysaccharides yield of 15.65%	624:666	The results showed that the highest polysaccharides yield of 15.65% was achieved with an extraction temperature of 54°C, pH 5.0, enzymatic treatment time of 93min and a liquid/material ratio of 29:1mL/g, which correlated well with the predicted yield of 15.58%.
27246376	8	55	from	agents	1451:1456	arg1	industries					1500:1509	the pharmaceutical and functional food industries	1461:1509	the pharmaceutical and functional food industries	1461:1509	In summary, this study provides an efficient enzyme-assisted extraction for LEPs, which can be used as natural antitumor agents in the pharmaceutical and functional food industries.
27246376	7	56	theme	HCT-116	1297:1303	arg1	cells					1314:1318	HCT-116 and HeLa cells	1297:1318	cells	1314:1318	In addition, the LEPs exhibited inhibition of cell proliferation on HCT-116 and HeLa cells in vitro.
27246376	3	57	theme	extraction	521:530	arg1	conditions					532:541	extraction conditions	521:541	extraction conditions	521:541	Then effects of extraction conditions were evaluated and optimized using a Box-Behnken design.
27246376	4	58	theme	54°C	715:718	arg1	temperature					700:710	an extraction temperature	686:710	an extraction temperature of 54°C	686:718	The results showed that the highest polysaccharides yield of 15.65% was achieved with an extraction temperature of 54°C, pH 5.0, enzymatic treatment time of 93min and a liquid/material ratio of 29:1mL/g, which correlated well with the predicted yield of 15.58%.
27246376	4	58	theme	54°C	715:718	arg1	time					749:752	enzymatic treatment time	729:752	enzymatic treatment time of 93min	729:761	The results showed that the highest polysaccharides yield of 15.65% was achieved with an extraction temperature of 54°C, pH 5.0, enzymatic treatment time of 93min and a liquid/material ratio of 29:1mL/g, which correlated well with the predicted yield of 15.58%.
27246376	4	58	theme	54°C	715:718	arg1	pH					721:722	pH 5.0	721:726	pH 5.0	721:726	The results showed that the highest polysaccharides yield of 15.65% was achieved with an extraction temperature of 54°C, pH 5.0, enzymatic treatment time of 93min and a liquid/material ratio of 29:1mL/g, which correlated well with the predicted yield of 15.58%.
27246376	4	58	theme	54°C	715:718	arg1	ratio					785:789	a liquid/material ratio	767:789	a liquid/material ratio of 29:1mL/g, which correlated well with the predicted yield of 15.58%	767:859	The results showed that the highest polysaccharides yield of 15.65% was achieved with an extraction temperature of 54°C, pH 5.0, enzymatic treatment time of 93min and a liquid/material ratio of 29:1mL/g, which correlated well with the predicted yield of 15.58%.
25965497	7	0	theme	nanocomposite	1030:1042	arg1	modulus					1010:1016	the storage modulus	998:1016	the storage modulus of PPMA/CNC nanocomposite containing 0.25 and 0.5 wt% cellulose	998:1080	The addition of NDC increased the storage modulus (11%) compared to neat PMMA at room temperature while the storage modulus of PPMA/CNC nanocomposite containing 0.25 and 0.5 wt% cellulose increased about 46% and 260% to that of the pure PMMA at the glass transition temperature, respectively.
25965497	10	1	from	research	1469:1476	arg1	applications					1560:1571	applications	1560:1571	applications such as: packaging, flexible screens, optically transparent films and light-weight transparent materials for ballistic protection	1560:1701	This work is intended to spur research and development activity for application of CNF reinforced PMMA nanocomposites in applications such as: packaging, flexible screens, optically transparent films and light-weight transparent materials for ballistic protection.
25965497	10	1	from	research	1469:1476	arg1	films					1633:1637	optically transparent films	1611:1637	optically transparent films	1611:1637	This work is intended to spur research and development activity for application of CNF reinforced PMMA nanocomposites in applications such as: packaging, flexible screens, optically transparent films and light-weight transparent materials for ballistic protection.
25965497	10	1	from	research	1469:1476	arg1	packaging					1582:1590	packaging	1582:1590	packaging	1582:1590	This work is intended to spur research and development activity for application of CNF reinforced PMMA nanocomposites in applications such as: packaging, flexible screens, optically transparent films and light-weight transparent materials for ballistic protection.
25965497	10	1	from	research	1469:1476	arg1	materials					1668:1676	light-weight transparent materials	1643:1676	light-weight transparent materials for ballistic protection	1643:1701	This work is intended to spur research and development activity for application of CNF reinforced PMMA nanocomposites in applications such as: packaging, flexible screens, optically transparent films and light-weight transparent materials for ballistic protection.
25965497	10	1	from	research	1469:1476	arg1	screens					1602:1608	flexible screens	1593:1608	flexible screens	1593:1608	This work is intended to spur research and development activity for application of CNF reinforced PMMA nanocomposites in applications such as: packaging, flexible screens, optically transparent films and light-weight transparent materials for ballistic protection.
25965497	3	2	theme	cellulose	447:455	arg1	nanofibrils					457:467	cellulose nanofibrils	447:467	cellulose nanofibrils	447:467	The loading of cellulose nanofibrils in the PMMA varied between 0.25 and 0.5 wt%.
25965497	5	3	theme	wt	699:700	arg1	level					711:715	the 0.5 wt% loading level	691:715	the 0.5 wt% loading level	691:715	The flexural modulus of the nanocomposites reinforced with NDC at the 0.5 wt% loading level increased 23% compared to that of pure PMMA.
25965497	5	4	theme	%	701:701	arg1	level					711:715	the 0.5 wt% loading level	691:715	the 0.5 wt% loading level	691:715	The flexural modulus of the nanocomposites reinforced with NDC at the 0.5 wt% loading level increased 23% compared to that of pure PMMA.
25965497	9	5	with	%	1372:1372	arg1	addition					1391:1398	the addition	1387:1398	the addition of 0.25 wt% CNC and NDC	1387:1422	The UV-vis transmittance of the CNF nanocomposites decreased by 9% and 27% with the addition of 0.25 wt% CNC and NDC, respectively.
25965497	3	6	theme	nanofibrils	457:467	arg1	loading					436:442	The loading	432:442	The loading of cellulose nanofibrils in the PMMA	432:479	The loading of cellulose nanofibrils in the PMMA varied between 0.25 and 0.5 wt%.
25965497	6	7	theme	MPa	889:891	arg1	strength					874:881	a flexural strength	863:881	a flexural strength of 57 MPa	863:891	The NFC composite also exhibited a slightly increased flexural strength around 60 MPa while PMMA had a flexural strength of 57 MPa.
25965497	5	8	theme	nanocomposites	653:666	arg1	modulus					638:644	The flexural modulus	625:644	The flexural modulus of the nanocomposites reinforced with NDC at the 0.5 wt% loading level	625:715	The flexural modulus of the nanocomposites reinforced with NDC at the 0.5 wt% loading level increased 23% compared to that of pure PMMA.
25965497	6	9	theme	increased	806:814	arg1	strength					825:832	a slightly increased flexural strength	795:832	a slightly increased flexural strength around 60 MPa	795:846	The NFC composite also exhibited a slightly increased flexural strength around 60 MPa while PMMA had a flexural strength of 57 MPa.
25965497	10	10	from	development	1482:1492	arg1	applications					1560:1571	applications	1560:1571	applications such as: packaging, flexible screens, optically transparent films and light-weight transparent materials for ballistic protection	1560:1701	This work is intended to spur research and development activity for application of CNF reinforced PMMA nanocomposites in applications such as: packaging, flexible screens, optically transparent films and light-weight transparent materials for ballistic protection.
25965497	10	10	from	development	1482:1492	arg1	films					1633:1637	optically transparent films	1611:1637	optically transparent films	1611:1637	This work is intended to spur research and development activity for application of CNF reinforced PMMA nanocomposites in applications such as: packaging, flexible screens, optically transparent films and light-weight transparent materials for ballistic protection.
25965497	10	10	from	development	1482:1492	arg1	packaging					1582:1590	packaging	1582:1590	packaging	1582:1590	This work is intended to spur research and development activity for application of CNF reinforced PMMA nanocomposites in applications such as: packaging, flexible screens, optically transparent films and light-weight transparent materials for ballistic protection.
25965497	10	10	from	development	1482:1492	arg1	materials					1668:1676	light-weight transparent materials	1643:1676	light-weight transparent materials for ballistic protection	1643:1701	This work is intended to spur research and development activity for application of CNF reinforced PMMA nanocomposites in applications such as: packaging, flexible screens, optically transparent films and light-weight transparent materials for ballistic protection.
25965497	10	10	from	development	1482:1492	arg1	screens					1602:1608	flexible screens	1593:1608	flexible screens	1593:1608	This work is intended to spur research and development activity for application of CNF reinforced PMMA nanocomposites in applications such as: packaging, flexible screens, optically transparent films and light-weight transparent materials for ballistic protection.
25965497	7	11	theme	%	1070:1070	arg1	cellulose					1072:1080	0.25 and 0.5 wt% cellulose	1055:1080	0.25 and 0.5 wt% cellulose	1055:1080	The addition of NDC increased the storage modulus (11%) compared to neat PMMA at room temperature while the storage modulus of PPMA/CNC nanocomposite containing 0.25 and 0.5 wt% cellulose increased about 46% and 260% to that of the pure PMMA at the glass transition temperature, respectively.
25965497	10	12	theme	reinforced	1526:1535	arg1	nanocomposites					1542:1555	CNF reinforced PMMA nanocomposites	1522:1555	CNF reinforced PMMA nanocomposites	1522:1555	This work is intended to spur research and development activity for application of CNF reinforced PMMA nanocomposites in applications such as: packaging, flexible screens, optically transparent films and light-weight transparent materials for ballistic protection.
25965497	7	13	theme	room	975:978	arg1	temperature					980:990	room temperature	975:990	room temperature	975:990	The addition of NDC increased the storage modulus (11%) compared to neat PMMA at room temperature while the storage modulus of PPMA/CNC nanocomposite containing 0.25 and 0.5 wt% cellulose increased about 46% and 260% to that of the pure PMMA at the glass transition temperature, respectively.
25965497	9	14	theme	NDC	1420:1422	arg1	addition					1391:1398	the addition	1387:1398	the addition of 0.25 wt% CNC and NDC	1387:1422	The UV-vis transmittance of the CNF nanocomposites decreased by 9% and 27% with the addition of 0.25 wt% CNC and NDC, respectively.
25965497	2	15	dep	cellulose	326:334	arg1	i					307:307	i	307:307	i	307:307	The nanofiber networks were prepared using three different types of cellulose nanofibers: (i) nanofibrillated cellulose (NFC), (ii) cellulose nanocrystals (CNC) and (iii) bacterial cellulose from nata de coca (NDC).
25965497	2	16	from	coca	420:423	arg1	cellulose					397:405	bacterial cellulose	387:405	(iii) bacterial cellulose from nata de coca (NDC)	381:429	The nanofiber networks were prepared using three different types of cellulose nanofibers: (i) nanofibrillated cellulose (NFC), (ii) cellulose nanocrystals (CNC) and (iii) bacterial cellulose from nata de coca (NDC).
25965497	2	16	from	coca	420:423	arg1	cellulose					326:334	(i) nanofibrillated cellulose	306:334	(i) nanofibrillated cellulose (NFC)	306:340	The nanofiber networks were prepared using three different types of cellulose nanofibers: (i) nanofibrillated cellulose (NFC), (ii) cellulose nanocrystals (CNC) and (iii) bacterial cellulose from nata de coca (NDC).
25965497	2	16	from	coca	420:423	arg1	nanofibers					294:303	cellulose nanofibers	284:303	cellulose nanofibers	284:303	The nanofiber networks were prepared using three different types of cellulose nanofibers: (i) nanofibrillated cellulose (NFC), (ii) cellulose nanocrystals (CNC) and (iii) bacterial cellulose from nata de coca (NDC).
25965497	2	16	from	coca	420:423	arg1	nanocrystals					358:369	cellulose nanocrystals	348:369	(ii) cellulose nanocrystals (CNC)	343:375	The nanofiber networks were prepared using three different types of cellulose nanofibers: (i) nanofibrillated cellulose (NFC), (ii) cellulose nanocrystals (CNC) and (iii) bacterial cellulose from nata de coca (NDC).
25965497	2	16	from	coca	420:423	arg1	NFC					337:339	NFC	337:339	NFC	337:339	The nanofiber networks were prepared using three different types of cellulose nanofibers: (i) nanofibrillated cellulose (NFC), (ii) cellulose nanocrystals (CNC) and (iii) bacterial cellulose from nata de coca (NDC).
25965497	2	16	from	coca	420:423	arg1	types					275:279	three different types	259:279	three different types of cellulose nanofibers: (i) nanofibrillated cellulose (NFC), (ii) cellulose nanocrystals (CNC) and (iii) bacterial cellulose from nata de coca (NDC)	259:429	The nanofiber networks were prepared using three different types of cellulose nanofibers: (i) nanofibrillated cellulose (NFC), (ii) cellulose nanocrystals (CNC) and (iii) bacterial cellulose from nata de coca (NDC).
25965497	2	16	from	coca	420:423	arg1	CNC					372:374	CNC	372:374	CNC	372:374	The nanofiber networks were prepared using three different types of cellulose nanofibers: (i) nanofibrillated cellulose (NFC), (ii) cellulose nanocrystals (CNC) and (iii) bacterial cellulose from nata de coca (NDC).
25965497	7	17	theme	neat	962:965	arg1	PMMA					967:970	neat PMMA	962:970	neat PMMA at room temperature while the storage modulus of PPMA/CNC nanocomposite containing 0.25 and 0.5 wt% cellulose increased about 46% and 260% to that of the pure PMMA at the glass transition temperature, respectively	962:1184	The addition of NDC increased the storage modulus (11%) compared to neat PMMA at room temperature while the storage modulus of PPMA/CNC nanocomposite containing 0.25 and 0.5 wt% cellulose increased about 46% and 260% to that of the pure PMMA at the glass transition temperature, respectively.
25965497	1	18	theme	polymethylmethacrylate	103:124	arg1	polymethylmethacrylate					103:124	polymethylmethacrylate	103:124	polymethylmethacrylate (PMMA)	103:131	Nanocomposites of polymethylmethacrylate (PMMA) and cellulose were made by a solution casting method using acetone as the solvent.
25965497	1	18	theme	polymethylmethacrylate	103:124	arg1	Nanocomposites					85:98	Nanocomposites	85:98	Nanocomposites of polymethylmethacrylate (PMMA) and cellulose	85:145	Nanocomposites of polymethylmethacrylate (PMMA) and cellulose were made by a solution casting method using acetone as the solvent.
25965497	1	18	theme	polymethylmethacrylate	103:124	arg1	cellulose					137:145	cellulose	137:145	cellulose	137:145	Nanocomposites of polymethylmethacrylate (PMMA) and cellulose were made by a solution casting method using acetone as the solvent.
25965497	4	19	theme	thermal	600:606	arg1	DMTA					618:621	DMTA	618:621	DMTA	618:621	The mechanical properties of the composites were evaluated using a dynamic mechanical thermal analyzer (DMTA).
25965497	4	19	theme	thermal	600:606	arg1	analyzer					608:615	a dynamic mechanical thermal analyzer	579:615	a dynamic mechanical thermal analyzer (DMTA)	579:622	The mechanical properties of the composites were evaluated using a dynamic mechanical thermal analyzer (DMTA).
25965497	4	20	theme	mechanical	518:527	arg1	properties					529:538	The mechanical properties	514:538	The mechanical properties of the composites	514:556	The mechanical properties of the composites were evaluated using a dynamic mechanical thermal analyzer (DMTA).
25965497	9	21	with	%	1380:1380	arg1	addition					1391:1398	the addition	1387:1398	the addition of 0.25 wt% CNC and NDC	1387:1422	The UV-vis transmittance of the CNF nanocomposites decreased by 9% and 27% with the addition of 0.25 wt% CNC and NDC, respectively.
25965497	2	22	dep	cellulose	397:405	arg1	iii					382:384	iii	382:384	iii	382:384	The nanofiber networks were prepared using three different types of cellulose nanofibers: (i) nanofibrillated cellulose (NFC), (ii) cellulose nanocrystals (CNC) and (iii) bacterial cellulose from nata de coca (NDC).
25965497	2	23	theme	nanofibers	294:303	arg1	types					275:279	three different types	259:279	three different types of cellulose nanofibers: (i) nanofibrillated cellulose (NFC), (ii) cellulose nanocrystals (CNC) and (iii) bacterial cellulose from nata de coca (NDC)	259:429	The nanofiber networks were prepared using three different types of cellulose nanofibers: (i) nanofibrillated cellulose (NFC), (ii) cellulose nanocrystals (CNC) and (iii) bacterial cellulose from nata de coca (NDC).
25965497	4	24	theme	mechanical	589:598	arg1	DMTA					618:621	DMTA	618:621	DMTA	618:621	The mechanical properties of the composites were evaluated using a dynamic mechanical thermal analyzer (DMTA).
25965497	4	24	theme	mechanical	589:598	arg1	analyzer					608:615	a dynamic mechanical thermal analyzer	579:615	a dynamic mechanical thermal analyzer (DMTA)	579:622	The mechanical properties of the composites were evaluated using a dynamic mechanical thermal analyzer (DMTA).
25965497	2	25	theme	de	417:418	arg1	NDC					426:428	NDC	426:428	NDC	426:428	The nanofiber networks were prepared using three different types of cellulose nanofibers: (i) nanofibrillated cellulose (NFC), (ii) cellulose nanocrystals (CNC) and (iii) bacterial cellulose from nata de coca (NDC).
25965497	2	25	theme	de	417:418	arg1	coca					420:423	nata de coca	412:423	nata de coca (NDC)	412:429	The nanofiber networks were prepared using three different types of cellulose nanofibers: (i) nanofibrillated cellulose (NFC), (ii) cellulose nanocrystals (CNC) and (iii) bacterial cellulose from nata de coca (NDC).
25965497	7	26	theme	pure	1126:1129	arg1	PMMA					1131:1134	the pure PMMA	1122:1134	the pure PMMA	1122:1134	The addition of NDC increased the storage modulus (11%) compared to neat PMMA at room temperature while the storage modulus of PPMA/CNC nanocomposite containing 0.25 and 0.5 wt% cellulose increased about 46% and 260% to that of the pure PMMA at the glass transition temperature, respectively.
25965497	3	27	theme	wt	509:510	arg1	%					511:511	0.25 and 0.5 wt%	496:511	0.25 and 0.5 wt%	496:511	The loading of cellulose nanofibrils in the PMMA varied between 0.25 and 0.5 wt%.
25965497	7	28	theme	glass	1143:1147	arg1	temperature					1160:1170	the glass transition temperature	1139:1170	the glass transition temperature	1139:1170	The addition of NDC increased the storage modulus (11%) compared to neat PMMA at room temperature while the storage modulus of PPMA/CNC nanocomposite containing 0.25 and 0.5 wt% cellulose increased about 46% and 260% to that of the pure PMMA at the glass transition temperature, respectively.
25965497	2	29	theme	nata	412:415	arg1	NDC					426:428	NDC	426:428	NDC	426:428	The nanofiber networks were prepared using three different types of cellulose nanofibers: (i) nanofibrillated cellulose (NFC), (ii) cellulose nanocrystals (CNC) and (iii) bacterial cellulose from nata de coca (NDC).
25965497	2	29	theme	nata	412:415	arg1	coca					420:423	nata de coca	412:423	nata de coca (NDC)	412:429	The nanofiber networks were prepared using three different types of cellulose nanofibers: (i) nanofibrillated cellulose (NFC), (ii) cellulose nanocrystals (CNC) and (iii) bacterial cellulose from nata de coca (NDC).
25965497	3	30	from	loading	436:442	arg1	PMMA					476:479	the PMMA	472:479	the PMMA	472:479	The loading of cellulose nanofibrils in the PMMA varied between 0.25 and 0.5 wt%.
25965497	2	31	theme	cellulose	326:334	arg1	types					275:279	three different types	259:279	three different types of cellulose nanofibers: (i) nanofibrillated cellulose (NFC), (ii) cellulose nanocrystals (CNC) and (iii) bacterial cellulose from nata de coca (NDC)	259:429	The nanofiber networks were prepared using three different types of cellulose nanofibers: (i) nanofibrillated cellulose (NFC), (ii) cellulose nanocrystals (CNC) and (iii) bacterial cellulose from nata de coca (NDC).
25965497	2	32	from	types	275:279	arg1	NDC					426:428	NDC	426:428	NDC	426:428	The nanofiber networks were prepared using three different types of cellulose nanofibers: (i) nanofibrillated cellulose (NFC), (ii) cellulose nanocrystals (CNC) and (iii) bacterial cellulose from nata de coca (NDC).
25965497	2	32	from	types	275:279	arg1	coca					420:423	nata de coca	412:423	nata de coca (NDC)	412:429	The nanofiber networks were prepared using three different types of cellulose nanofibers: (i) nanofibrillated cellulose (NFC), (ii) cellulose nanocrystals (CNC) and (iii) bacterial cellulose from nata de coca (NDC).
25965497	6	33	theme	NFC	766:768	arg1	composite					770:778	The NFC composite	762:778	The NFC composite	762:778	The NFC composite also exhibited a slightly increased flexural strength around 60 MPa while PMMA had a flexural strength of 57 MPa.
25965497	7	34	theme	PPMA/CNC	1021:1028	arg1	nanocomposite					1030:1042	PPMA/CNC nanocomposite	1021:1042	PPMA/CNC nanocomposite containing 0.25 and 0.5 wt% cellulose	1021:1080	The addition of NDC increased the storage modulus (11%) compared to neat PMMA at room temperature while the storage modulus of PPMA/CNC nanocomposite containing 0.25 and 0.5 wt% cellulose increased about 46% and 260% to that of the pure PMMA at the glass transition temperature, respectively.
25965497	6	35	theme	flexural	865:872	arg1	strength					874:881	a flexural strength	863:881	a flexural strength of 57 MPa	863:891	The NFC composite also exhibited a slightly increased flexural strength around 60 MPa while PMMA had a flexural strength of 57 MPa.
25965497	5	36	theme	flexural	629:636	arg1	modulus					638:644	The flexural modulus	625:644	The flexural modulus of the nanocomposites reinforced with NDC at the 0.5 wt% loading level	625:715	The flexural modulus of the nanocomposites reinforced with NDC at the 0.5 wt% loading level increased 23% compared to that of pure PMMA.
25965497	7	37	contain	containing	1044:1053	arg1	nanocomposite					1030:1042	PPMA/CNC nanocomposite	1021:1042	PPMA/CNC nanocomposite containing 0.25 and 0.5 wt% cellulose	1021:1080	The addition of NDC increased the storage modulus (11%) compared to neat PMMA at room temperature while the storage modulus of PPMA/CNC nanocomposite containing 0.25 and 0.5 wt% cellulose increased about 46% and 260% to that of the pure PMMA at the glass transition temperature, respectively.
25965497	7	37	contain	containing	1044:1053	arg2	cellulose					1072:1080	0.25 and 0.5 wt% cellulose	1055:1080	0.25 and 0.5 wt% cellulose	1055:1080	The addition of NDC increased the storage modulus (11%) compared to neat PMMA at room temperature while the storage modulus of PPMA/CNC nanocomposite containing 0.25 and 0.5 wt% cellulose increased about 46% and 260% to that of the pure PMMA at the glass transition temperature, respectively.
25965497	2	38	theme	nanofibrillated	310:324	arg1	NFC					337:339	NFC	337:339	NFC	337:339	The nanofiber networks were prepared using three different types of cellulose nanofibers: (i) nanofibrillated cellulose (NFC), (ii) cellulose nanocrystals (CNC) and (iii) bacterial cellulose from nata de coca (NDC).
25965497	2	38	theme	nanofibrillated	310:324	arg1	cellulose					326:334	(i) nanofibrillated cellulose	306:334	(i) nanofibrillated cellulose (NFC)	306:340	The nanofiber networks were prepared using three different types of cellulose nanofibers: (i) nanofibrillated cellulose (NFC), (ii) cellulose nanocrystals (CNC) and (iii) bacterial cellulose from nata de coca (NDC).
25965497	9	39	theme	wt	1408:1409	arg1	CNC					1412:1414	0.25 wt% CNC	1403:1414	0.25 wt% CNC	1403:1414	The UV-vis transmittance of the CNF nanocomposites decreased by 9% and 27% with the addition of 0.25 wt% CNC and NDC, respectively.
25965497	2	40	theme	bacterial	387:395	arg1	cellulose					397:405	bacterial cellulose	387:405	(iii) bacterial cellulose from nata de coca (NDC)	381:429	The nanofiber networks were prepared using three different types of cellulose nanofibers: (i) nanofibrillated cellulose (NFC), (ii) cellulose nanocrystals (CNC) and (iii) bacterial cellulose from nata de coca (NDC).
25965497	2	41	theme	cellulose	397:405	arg1	types					275:279	three different types	259:279	three different types of cellulose nanofibers: (i) nanofibrillated cellulose (NFC), (ii) cellulose nanocrystals (CNC) and (iii) bacterial cellulose from nata de coca (NDC)	259:429	The nanofiber networks were prepared using three different types of cellulose nanofibers: (i) nanofibrillated cellulose (NFC), (ii) cellulose nanocrystals (CNC) and (iii) bacterial cellulose from nata de coca (NDC).
25965497	9	42	theme	UV-vis	1311:1316	arg1	transmittance					1318:1330	The UV-vis transmittance	1307:1330	The UV-vis transmittance of the CNF	1307:1341	The UV-vis transmittance of the CNF nanocomposites decreased by 9% and 27% with the addition of 0.25 wt% CNC and NDC, respectively.
25965497	2	43	dep	nanocrystals	358:369	arg1	ii					344:345	ii	344:345	ii	344:345	The nanofiber networks were prepared using three different types of cellulose nanofibers: (i) nanofibrillated cellulose (NFC), (ii) cellulose nanocrystals (CNC) and (iii) bacterial cellulose from nata de coca (NDC).
25965497	10	44	theme	ballistic	1682:1690	arg1	protection					1692:1701	ballistic protection	1682:1701	ballistic protection	1682:1701	This work is intended to spur research and development activity for application of CNF reinforced PMMA nanocomposites in applications such as: packaging, flexible screens, optically transparent films and light-weight transparent materials for ballistic protection.
25965497	1	45	theme	cellulose	137:145	arg1	polymethylmethacrylate					103:124	polymethylmethacrylate	103:124	polymethylmethacrylate (PMMA)	103:131	Nanocomposites of polymethylmethacrylate (PMMA) and cellulose were made by a solution casting method using acetone as the solvent.
25965497	1	45	theme	cellulose	137:145	arg1	Nanocomposites					85:98	Nanocomposites	85:98	Nanocomposites of polymethylmethacrylate (PMMA) and cellulose	85:145	Nanocomposites of polymethylmethacrylate (PMMA) and cellulose were made by a solution casting method using acetone as the solvent.
25965497	1	45	theme	cellulose	137:145	arg1	cellulose					137:145	cellulose	137:145	cellulose	137:145	Nanocomposites of polymethylmethacrylate (PMMA) and cellulose were made by a solution casting method using acetone as the solvent.
25965497	1	46	theme	solution	162:169	arg1	method					179:184	a solution casting method	160:184	a solution casting method using acetone as the solvent	160:213	Nanocomposites of polymethylmethacrylate (PMMA) and cellulose were made by a solution casting method using acetone as the solvent.
25965497	0	47	theme	PMMA-cellulose-based	48:67	arg1	nanocomposites					69:82	transparent PMMA-cellulose-based nanocomposites	36:82	transparent PMMA-cellulose-based nanocomposites	36:82	Preparation and characterization of transparent PMMA-cellulose-based nanocomposites.
25965497	5	48	theme	pure	751:754	arg1	PMMA					756:759	pure PMMA	751:759	pure PMMA	751:759	The flexural modulus of the nanocomposites reinforced with NDC at the 0.5 wt% loading level increased 23% compared to that of pure PMMA.
25965497	6	49	contain	had	859:861	arg2	strength					874:881	a flexural strength	863:881	a flexural strength of 57 MPa	863:891	The NFC composite also exhibited a slightly increased flexural strength around 60 MPa while PMMA had a flexural strength of 57 MPa.
25965497	6	49	contain	had	859:861	arg1	PMMA					854:857	PMMA	854:857	PMMA	854:857	The NFC composite also exhibited a slightly increased flexural strength around 60 MPa while PMMA had a flexural strength of 57 MPa.
25965497	7	50	theme	transition	1149:1158	arg1	temperature					1160:1170	the glass transition temperature	1139:1170	the glass transition temperature	1139:1170	The addition of NDC increased the storage modulus (11%) compared to neat PMMA at room temperature while the storage modulus of PPMA/CNC nanocomposite containing 0.25 and 0.5 wt% cellulose increased about 46% and 260% to that of the pure PMMA at the glass transition temperature, respectively.
25965497	4	51	theme	dynamic	581:587	arg1	DMTA					618:621	DMTA	618:621	DMTA	618:621	The mechanical properties of the composites were evaluated using a dynamic mechanical thermal analyzer (DMTA).
25965497	4	51	theme	dynamic	581:587	arg1	analyzer					608:615	a dynamic mechanical thermal analyzer	579:615	a dynamic mechanical thermal analyzer (DMTA)	579:622	The mechanical properties of the composites were evaluated using a dynamic mechanical thermal analyzer (DMTA).
25965497	0	52	theme	transparent	36:46	arg1	nanocomposites					69:82	transparent PMMA-cellulose-based nanocomposites	36:82	transparent PMMA-cellulose-based nanocomposites	36:82	Preparation and characterization of transparent PMMA-cellulose-based nanocomposites.
25965497	5	53	theme	loading	703:709	arg1	level					711:715	the 0.5 wt% loading level	691:715	the 0.5 wt% loading level	691:715	The flexural modulus of the nanocomposites reinforced with NDC at the 0.5 wt% loading level increased 23% compared to that of pure PMMA.
25965497	8	54	theme	composites	1295:1304	arg1	stability					1278:1286	thermal stability	1270:1286	thermal stability of the composites	1270:1304	Thermogravimetric analysis (TGA) indicated that there was no significant change in thermal stability of the composites.
25965497	9	55	theme	%	1410:1410	arg1	CNC					1412:1414	0.25 wt% CNC	1403:1414	0.25 wt% CNC	1403:1414	The UV-vis transmittance of the CNF nanocomposites decreased by 9% and 27% with the addition of 0.25 wt% CNC and NDC, respectively.
25965497	10	56	dep	research	1469:1476	arg1	activity					1494:1501	activity	1494:1501	activity	1494:1501	This work is intended to spur research and development activity for application of CNF reinforced PMMA nanocomposites in applications such as: packaging, flexible screens, optically transparent films and light-weight transparent materials for ballistic protection.
25965497	7	57	theme	wt	1068:1069	arg1	cellulose					1072:1080	0.25 and 0.5 wt% cellulose	1055:1080	0.25 and 0.5 wt% cellulose	1055:1080	The addition of NDC increased the storage modulus (11%) compared to neat PMMA at room temperature while the storage modulus of PPMA/CNC nanocomposite containing 0.25 and 0.5 wt% cellulose increased about 46% and 260% to that of the pure PMMA at the glass transition temperature, respectively.
25965497	7	58	theme	storage	928:934	arg1	modulus					936:942	the storage modulus	924:942	the storage modulus (11%)	924:948	The addition of NDC increased the storage modulus (11%) compared to neat PMMA at room temperature while the storage modulus of PPMA/CNC nanocomposite containing 0.25 and 0.5 wt% cellulose increased about 46% and 260% to that of the pure PMMA at the glass transition temperature, respectively.
25965497	7	58	theme	storage	928:934	arg1	%					947:947	11%	945:947	11%	945:947	The addition of NDC increased the storage modulus (11%) compared to neat PMMA at room temperature while the storage modulus of PPMA/CNC nanocomposite containing 0.25 and 0.5 wt% cellulose increased about 46% and 260% to that of the pure PMMA at the glass transition temperature, respectively.
25965497	10	59	theme	transparent	1656:1666	arg1	materials					1668:1676	light-weight transparent materials	1643:1676	light-weight transparent materials for ballistic protection	1643:1701	This work is intended to spur research and development activity for application of CNF reinforced PMMA nanocomposites in applications such as: packaging, flexible screens, optically transparent films and light-weight transparent materials for ballistic protection.
25965497	9	60	theme	CNC	1412:1414	arg1	addition					1391:1398	the addition	1387:1398	the addition of 0.25 wt% CNC and NDC	1387:1422	The UV-vis transmittance of the CNF nanocomposites decreased by 9% and 27% with the addition of 0.25 wt% CNC and NDC, respectively.
25965497	7	61	theme	NDC	910:912	arg1	addition					898:905	The addition	894:905	The addition of NDC	894:912	The addition of NDC increased the storage modulus (11%) compared to neat PMMA at room temperature while the storage modulus of PPMA/CNC nanocomposite containing 0.25 and 0.5 wt% cellulose increased about 46% and 260% to that of the pure PMMA at the glass transition temperature, respectively.
25965497	0	62	theme	nanocomposites	69:82	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of transparent PMMA-cellulose-based nanocomposites.
25965497	0	62	theme	nanocomposites	69:82	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of transparent PMMA-cellulose-based nanocomposites.
25965497	7	63	from	temperature	980:990	arg1	PMMA					967:970	neat PMMA	962:970	neat PMMA at room temperature while the storage modulus of PPMA/CNC nanocomposite containing 0.25 and 0.5 wt% cellulose increased about 46% and 260% to that of the pure PMMA at the glass transition temperature, respectively	962:1184	The addition of NDC increased the storage modulus (11%) compared to neat PMMA at room temperature while the storage modulus of PPMA/CNC nanocomposite containing 0.25 and 0.5 wt% cellulose increased about 46% and 260% to that of the pure PMMA at the glass transition temperature, respectively.
25965497	10	64	theme	light-weight	1643:1654	arg1	materials					1668:1676	light-weight transparent materials	1643:1676	light-weight transparent materials for ballistic protection	1643:1701	This work is intended to spur research and development activity for application of CNF reinforced PMMA nanocomposites in applications such as: packaging, flexible screens, optically transparent films and light-weight transparent materials for ballistic protection.
25965497	6	65	theme	flexural	816:823	arg1	strength					825:832	a slightly increased flexural strength	795:832	a slightly increased flexural strength around 60 MPa	795:846	The NFC composite also exhibited a slightly increased flexural strength around 60 MPa while PMMA had a flexural strength of 57 MPa.
25965497	10	66	theme	flexible	1593:1600	arg1	screens					1602:1608	flexible screens	1593:1608	flexible screens	1593:1608	This work is intended to spur research and development activity for application of CNF reinforced PMMA nanocomposites in applications such as: packaging, flexible screens, optically transparent films and light-weight transparent materials for ballistic protection.
25965497	9	67	dep	nanocomposites	1343:1356	arg1	decreased					1358:1366	decreased	1358:1366	nanocomposites decreased by 9% and 27% with the addition of 0.25 wt% CNC and NDC, respectively	1343:1436	The UV-vis transmittance of the CNF nanocomposites decreased by 9% and 27% with the addition of 0.25 wt% CNC and NDC, respectively.
25965497	10	68	theme	transparent	1621:1631	arg1	films					1633:1637	optically transparent films	1611:1637	optically transparent films	1611:1637	This work is intended to spur research and development activity for application of CNF reinforced PMMA nanocomposites in applications such as: packaging, flexible screens, optically transparent films and light-weight transparent materials for ballistic protection.
25965497	7	69	theme	storage	1002:1008	arg1	modulus					1010:1016	the storage modulus	998:1016	the storage modulus of PPMA/CNC nanocomposite containing 0.25 and 0.5 wt% cellulose	998:1080	The addition of NDC increased the storage modulus (11%) compared to neat PMMA at room temperature while the storage modulus of PPMA/CNC nanocomposite containing 0.25 and 0.5 wt% cellulose increased about 46% and 260% to that of the pure PMMA at the glass transition temperature, respectively.
25965497	2	70	theme	nanofiber	220:228	arg1	networks					230:237	The nanofiber networks	216:237	The nanofiber networks	216:237	The nanofiber networks were prepared using three different types of cellulose nanofibers: (i) nanofibrillated cellulose (NFC), (ii) cellulose nanocrystals (CNC) and (iii) bacterial cellulose from nata de coca (NDC).
25965497	2	71	theme	cellulose	284:292	arg1	nanofibers					294:303	cellulose nanofibers	284:303	cellulose nanofibers	284:303	The nanofiber networks were prepared using three different types of cellulose nanofibers: (i) nanofibrillated cellulose (NFC), (ii) cellulose nanocrystals (CNC) and (iii) bacterial cellulose from nata de coca (NDC).
25965497	9	72	theme	CNF	1339:1341	arg1	transmittance					1318:1330	The UV-vis transmittance	1307:1330	The UV-vis transmittance of the CNF	1307:1341	The UV-vis transmittance of the CNF nanocomposites decreased by 9% and 27% with the addition of 0.25 wt% CNC and NDC, respectively.
25965497	10	73	theme	PMMA	1537:1540	arg1	nanocomposites					1542:1555	CNF reinforced PMMA nanocomposites	1522:1555	CNF reinforced PMMA nanocomposites	1522:1555	This work is intended to spur research and development activity for application of CNF reinforced PMMA nanocomposites in applications such as: packaging, flexible screens, optically transparent films and light-weight transparent materials for ballistic protection.
25965497	10	74	theme	CNF	1522:1524	arg1	nanocomposites					1542:1555	CNF reinforced PMMA nanocomposites	1522:1555	CNF reinforced PMMA nanocomposites	1522:1555	This work is intended to spur research and development activity for application of CNF reinforced PMMA nanocomposites in applications such as: packaging, flexible screens, optically transparent films and light-weight transparent materials for ballistic protection.
25965497	8	75	theme	Thermogravimetric	1187:1203	arg1	TGA					1215:1217	TGA	1215:1217	TGA	1215:1217	Thermogravimetric analysis (TGA) indicated that there was no significant change in thermal stability of the composites.
25965497	8	75	theme	Thermogravimetric	1187:1203	arg1	analysis					1205:1212	Thermogravimetric analysis	1187:1212	Thermogravimetric analysis (TGA)	1187:1218	Thermogravimetric analysis (TGA) indicated that there was no significant change in thermal stability of the composites.
25965497	8	76	theme	significant	1248:1258	arg1	change					1260:1265	no significant change	1245:1265	no significant change	1245:1265	Thermogravimetric analysis (TGA) indicated that there was no significant change in thermal stability of the composites.
25965497	1	77	theme	casting	171:177	arg1	method					179:184	a solution casting method	160:184	a solution casting method using acetone as the solvent	160:213	Nanocomposites of polymethylmethacrylate (PMMA) and cellulose were made by a solution casting method using acetone as the solvent.
25965497	8	78	theme	thermal	1270:1276	arg1	stability					1278:1286	thermal stability	1270:1286	thermal stability of the composites	1270:1304	Thermogravimetric analysis (TGA) indicated that there was no significant change in thermal stability of the composites.
25965497	2	79	theme	nanocrystals	358:369	arg1	types					275:279	three different types	259:279	three different types of cellulose nanofibers: (i) nanofibrillated cellulose (NFC), (ii) cellulose nanocrystals (CNC) and (iii) bacterial cellulose from nata de coca (NDC)	259:429	The nanofiber networks were prepared using three different types of cellulose nanofibers: (i) nanofibrillated cellulose (NFC), (ii) cellulose nanocrystals (CNC) and (iii) bacterial cellulose from nata de coca (NDC).
25965497	10	80	theme	nanocomposites	1542:1555	arg1	application					1507:1517	application	1507:1517	application of CNF reinforced PMMA nanocomposites	1507:1555	This work is intended to spur research and development activity for application of CNF reinforced PMMA nanocomposites in applications such as: packaging, flexible screens, optically transparent films and light-weight transparent materials for ballistic protection.
25965497	5	81	from	level	711:715	arg1	NDC					684:686	NDC	684:686	NDC at the 0.5 wt% loading level	684:715	The flexural modulus of the nanocomposites reinforced with NDC at the 0.5 wt% loading level increased 23% compared to that of pure PMMA.
25965497	2	82	theme	different	265:273	arg1	types					275:279	three different types	259:279	three different types of cellulose nanofibers: (i) nanofibrillated cellulose (NFC), (ii) cellulose nanocrystals (CNC) and (iii) bacterial cellulose from nata de coca (NDC)	259:429	The nanofiber networks were prepared using three different types of cellulose nanofibers: (i) nanofibrillated cellulose (NFC), (ii) cellulose nanocrystals (CNC) and (iii) bacterial cellulose from nata de coca (NDC).
25965497	2	83	theme	cellulose	348:356	arg1	CNC					372:374	CNC	372:374	CNC	372:374	The nanofiber networks were prepared using three different types of cellulose nanofibers: (i) nanofibrillated cellulose (NFC), (ii) cellulose nanocrystals (CNC) and (iii) bacterial cellulose from nata de coca (NDC).
25965497	2	83	theme	cellulose	348:356	arg1	nanocrystals					358:369	cellulose nanocrystals	348:369	(ii) cellulose nanocrystals (CNC)	343:375	The nanofiber networks were prepared using three different types of cellulose nanofibers: (i) nanofibrillated cellulose (NFC), (ii) cellulose nanocrystals (CNC) and (iii) bacterial cellulose from nata de coca (NDC).
25965497	4	84	theme	composites	547:556	arg1	properties					529:538	The mechanical properties	514:538	The mechanical properties of the composites	514:556	The mechanical properties of the composites were evaluated using a dynamic mechanical thermal analyzer (DMTA).
28385238	1	0	theme	hydrogel	177:184	arg1	system					186:191	a biomimetic injectable hydrogel system	153:191	a biomimetic injectable hydrogel system based on hyaluronic acid-adipic dihydrazide and the oligopeptide G4RGDS-grafted oxidized pectin, in which their hydrazide and aldehyde-derivatives enable covalent hydrazone crosslinking of polysaccharides	153:396	In the present study, we developed a biomimetic injectable hydrogel system based on hyaluronic acid-adipic dihydrazide and the oligopeptide G4RGDS-grafted oxidized pectin, in which their hydrazide and aldehyde-derivatives enable covalent hydrazone crosslinking of polysaccharides.
28385238	2	1	theme	regenerative	563:574	arg1	purposes					576:583	regenerative purposes	563:583	regenerative purposes	563:583	The hydrazone crosslinking strategy is simple, while circumventing toxicity, making this injectable system feasible, minimally invasive and easily translatable for regenerative purposes.
28385238	7	2	theme	useful	1320:1325	arg1	hydrogel					1278:1285	the novel injectable multicomponent hydrogel	1242:1285	the novel injectable multicomponent hydrogel presented here	1242:1300	Overall, the novel injectable multicomponent hydrogel presented here is expected to be useful biomaterial scaffold for cartilage tissue regeneration.
28385238	7	2	theme	useful	1320:1325	arg1	scaffold					1339:1346	useful biomaterial scaffold	1320:1346	useful biomaterial scaffold for cartilage tissue regeneration	1320:1380	Overall, the novel injectable multicomponent hydrogel presented here is expected to be useful biomaterial scaffold for cartilage tissue regeneration.
28385238	4	3	theme	HA/pectin	810:818	arg1	composition					820:830	HA/pectin composition	810:830	HA/pectin composition	810:830	Additionally, the preliminary studies demonstrated that chondrocyte behavior was dependent on HA/pectin composition and the presence of integrin binding moieties.
28385238	7	4	theme	tissue	1362:1367	arg1	regeneration					1369:1380	cartilage tissue regeneration	1352:1380	cartilage tissue regeneration	1352:1380	Overall, the novel injectable multicomponent hydrogel presented here is expected to be useful biomaterial scaffold for cartilage tissue regeneration.
28385238	3	5	theme	weight	603:608	arg1	ratios					610:615	their weight ratios	597:615	their weight ratios	597:615	By varying their weight ratios, the physicochemical properties of the mechanically stable hydrogel system were easily adjustable.
28385238	3	6	theme	physicochemical	622:636	arg1	properties					638:647	the physicochemical properties	618:647	the physicochemical properties of the mechanically stable hydrogel system	618:690	By varying their weight ratios, the physicochemical properties of the mechanically stable hydrogel system were easily adjustable.
28385238	3	6	theme	physicochemical	622:636	arg1	adjustable					704:713	adjustable	704:713	adjustable	704:713	By varying their weight ratios, the physicochemical properties of the mechanically stable hydrogel system were easily adjustable.
28385238	6	7	theme	tissue	1162:1167	arg1	compatibility					1169:1181	acceptable tissue compatibility	1151:1181	acceptable tissue compatibility	1151:1181	Furthermore, the hydrogel system exhibited acceptable tissue compatibility by using a mouse subcutaneous implantation model.
28385238	6	8	theme	implantation	1213:1224	arg1	model					1226:1230	a mouse subcutaneous implantation model	1192:1230	a mouse subcutaneous implantation model	1192:1230	Furthermore, the hydrogel system exhibited acceptable tissue compatibility by using a mouse subcutaneous implantation model.
28385238	4	9	theme	chondrocyte	772:782	arg1	behavior					784:791	chondrocyte behavior	772:791	chondrocyte behavior	772:791	Additionally, the preliminary studies demonstrated that chondrocyte behavior was dependent on HA/pectin composition and the presence of integrin binding moieties.
28385238	5	10	theme	chondrocyte	1054:1064	arg1	phenotype					1066:1074	chondrocyte phenotype	1054:1074	chondrocyte phenotype	1054:1074	Specifically, the incorporation of a certain amount of G4RGDS oligopeptide into HA/pectin-based hydrogels could serve as a biologically active microenvironment that supported chondrocyte phenotype and facilitated chondrogenesis.
28385238	2	11	dep	feasible	506:513	arg1	invasive					526:533	invasive	526:533	invasive	526:533	The hydrazone crosslinking strategy is simple, while circumventing toxicity, making this injectable system feasible, minimally invasive and easily translatable for regenerative purposes.
28385238	6	12	theme	subcutaneous	1200:1211	arg1	model					1226:1230	a mouse subcutaneous implantation model	1192:1230	a mouse subcutaneous implantation model	1192:1230	Furthermore, the hydrogel system exhibited acceptable tissue compatibility by using a mouse subcutaneous implantation model.
28385238	6	13	theme	hydrogel	1125:1132	arg1	system					1134:1139	the hydrogel system	1121:1139	the hydrogel system	1121:1139	Furthermore, the hydrogel system exhibited acceptable tissue compatibility by using a mouse subcutaneous implantation model.
28385238	4	14	theme	binding	861:867	arg1	moieties					869:876	integrin binding moieties	852:876	integrin binding moieties	852:876	Additionally, the preliminary studies demonstrated that chondrocyte behavior was dependent on HA/pectin composition and the presence of integrin binding moieties.
28385238	1	15	theme	hyaluronic	202:211	arg1	dihydrazide					225:235	hyaluronic acid-adipic dihydrazide	202:235	hyaluronic acid-adipic dihydrazide	202:235	In the present study, we developed a biomimetic injectable hydrogel system based on hyaluronic acid-adipic dihydrazide and the oligopeptide G4RGDS-grafted oxidized pectin, in which their hydrazide and aldehyde-derivatives enable covalent hydrazone crosslinking of polysaccharides.
28385238	5	16	theme	G4RGDS	934:939	arg1	oligopeptide					941:952	G4RGDS oligopeptide	934:952	G4RGDS oligopeptide	934:952	Specifically, the incorporation of a certain amount of G4RGDS oligopeptide into HA/pectin-based hydrogels could serve as a biologically active microenvironment that supported chondrocyte phenotype and facilitated chondrogenesis.
28385238	6	17	theme	acceptable	1151:1160	arg1	compatibility					1169:1181	acceptable tissue compatibility	1151:1181	acceptable tissue compatibility	1151:1181	Furthermore, the hydrogel system exhibited acceptable tissue compatibility by using a mouse subcutaneous implantation model.
28385238	7	18	theme	novel	1246:1250	arg1	hydrogel					1278:1285	the novel injectable multicomponent hydrogel	1242:1285	the novel injectable multicomponent hydrogel presented here	1242:1300	Overall, the novel injectable multicomponent hydrogel presented here is expected to be useful biomaterial scaffold for cartilage tissue regeneration.
28385238	7	18	theme	novel	1246:1250	arg1	scaffold					1339:1346	useful biomaterial scaffold	1320:1346	useful biomaterial scaffold for cartilage tissue regeneration	1320:1380	Overall, the novel injectable multicomponent hydrogel presented here is expected to be useful biomaterial scaffold for cartilage tissue regeneration.
28385238	3	19	theme	stable	669:674	arg1	system					685:690	the mechanically stable hydrogel system	652:690	the mechanically stable hydrogel system	652:690	By varying their weight ratios, the physicochemical properties of the mechanically stable hydrogel system were easily adjustable.
28385238	1	20	theme	acid-adipic	213:223	arg1	dihydrazide					225:235	hyaluronic acid-adipic dihydrazide	202:235	hyaluronic acid-adipic dihydrazide	202:235	In the present study, we developed a biomimetic injectable hydrogel system based on hyaluronic acid-adipic dihydrazide and the oligopeptide G4RGDS-grafted oxidized pectin, in which their hydrazide and aldehyde-derivatives enable covalent hydrazone crosslinking of polysaccharides.
28385238	0	21	theme	hyaluronic	33:42	arg1	hydrogel					75:82	injectable hyaluronic acid/RGD-functionalized pectin hydrogel	22:82	injectable hyaluronic acid/RGD-functionalized pectin hydrogel	22:82	Self-crosslinking and injectable hyaluronic acid/RGD-functionalized pectin hydrogel for cartilage tissue engineering.
28385238	4	22	theme	integrin	852:859	arg1	moieties					869:876	integrin binding moieties	852:876	integrin binding moieties	852:876	Additionally, the preliminary studies demonstrated that chondrocyte behavior was dependent on HA/pectin composition and the presence of integrin binding moieties.
28385238	7	23	theme	injectable	1252:1261	arg1	hydrogel					1278:1285	the novel injectable multicomponent hydrogel	1242:1285	the novel injectable multicomponent hydrogel presented here	1242:1300	Overall, the novel injectable multicomponent hydrogel presented here is expected to be useful biomaterial scaffold for cartilage tissue regeneration.
28385238	7	23	theme	injectable	1252:1261	arg1	scaffold					1339:1346	useful biomaterial scaffold	1320:1346	useful biomaterial scaffold for cartilage tissue regeneration	1320:1380	Overall, the novel injectable multicomponent hydrogel presented here is expected to be useful biomaterial scaffold for cartilage tissue regeneration.
28385238	1	24	theme	present	125:131	arg1	study					133:137	the present study	121:137	the present study	121:137	In the present study, we developed a biomimetic injectable hydrogel system based on hyaluronic acid-adipic dihydrazide and the oligopeptide G4RGDS-grafted oxidized pectin, in which their hydrazide and aldehyde-derivatives enable covalent hydrazone crosslinking of polysaccharides.
28385238	4	25	theme	preliminary	734:744	arg1	studies					746:752	the preliminary studies	730:752	the preliminary studies	730:752	Additionally, the preliminary studies demonstrated that chondrocyte behavior was dependent on HA/pectin composition and the presence of integrin binding moieties.
28385238	0	26	theme	injectable	22:31	arg1	hydrogel					75:82	injectable hyaluronic acid/RGD-functionalized pectin hydrogel	22:82	injectable hyaluronic acid/RGD-functionalized pectin hydrogel	22:82	Self-crosslinking and injectable hyaluronic acid/RGD-functionalized pectin hydrogel for cartilage tissue engineering.
28385238	7	27	theme	multicomponent	1263:1276	arg1	hydrogel					1278:1285	the novel injectable multicomponent hydrogel	1242:1285	the novel injectable multicomponent hydrogel presented here	1242:1300	Overall, the novel injectable multicomponent hydrogel presented here is expected to be useful biomaterial scaffold for cartilage tissue regeneration.
28385238	7	27	theme	multicomponent	1263:1276	arg1	scaffold					1339:1346	useful biomaterial scaffold	1320:1346	useful biomaterial scaffold for cartilage tissue regeneration	1320:1380	Overall, the novel injectable multicomponent hydrogel presented here is expected to be useful biomaterial scaffold for cartilage tissue regeneration.
28385238	4	28	theme	moieties	869:876	arg1	composition					820:830	HA/pectin composition	810:830	HA/pectin composition	810:830	Additionally, the preliminary studies demonstrated that chondrocyte behavior was dependent on HA/pectin composition and the presence of integrin binding moieties.
28385238	4	28	theme	moieties	869:876	arg1	presence					840:847	the presence	836:847	the presence of integrin binding moieties	836:876	Additionally, the preliminary studies demonstrated that chondrocyte behavior was dependent on HA/pectin composition and the presence of integrin binding moieties.
28385238	2	29	theme	injectable	488:497	arg1	system					499:504	this injectable system	483:504	this injectable system feasible, minimally invasive	483:533	The hydrazone crosslinking strategy is simple, while circumventing toxicity, making this injectable system feasible, minimally invasive and easily translatable for regenerative purposes.
28385238	0	30	theme	pectin	68:73	arg1	hydrogel					75:82	injectable hyaluronic acid/RGD-functionalized pectin hydrogel	22:82	injectable hyaluronic acid/RGD-functionalized pectin hydrogel	22:82	Self-crosslinking and injectable hyaluronic acid/RGD-functionalized pectin hydrogel for cartilage tissue engineering.
28385238	1	31	theme	covalent	347:354	arg1	crosslinking					366:377	covalent hydrazone crosslinking	347:377	covalent hydrazone crosslinking of polysaccharides	347:396	In the present study, we developed a biomimetic injectable hydrogel system based on hyaluronic acid-adipic dihydrazide and the oligopeptide G4RGDS-grafted oxidized pectin, in which their hydrazide and aldehyde-derivatives enable covalent hydrazone crosslinking of polysaccharides.
28385238	5	32	theme	active	1015:1020	arg1	microenvironment					1022:1037	a biologically active microenvironment	1000:1037	a biologically active microenvironment that supported chondrocyte phenotype and facilitated chondrogenesis	1000:1105	Specifically, the incorporation of a certain amount of G4RGDS oligopeptide into HA/pectin-based hydrogels could serve as a biologically active microenvironment that supported chondrocyte phenotype and facilitated chondrogenesis.
28385238	5	32	theme	active	1015:1020	arg1	incorporation					897:909	the incorporation	893:909	the incorporation of a certain amount of G4RGDS oligopeptide into HA/pectin-based hydrogels	893:983	Specifically, the incorporation of a certain amount of G4RGDS oligopeptide into HA/pectin-based hydrogels could serve as a biologically active microenvironment that supported chondrocyte phenotype and facilitated chondrogenesis.
28385238	7	33	theme	biomaterial	1327:1337	arg1	hydrogel					1278:1285	the novel injectable multicomponent hydrogel	1242:1285	the novel injectable multicomponent hydrogel presented here	1242:1300	Overall, the novel injectable multicomponent hydrogel presented here is expected to be useful biomaterial scaffold for cartilage tissue regeneration.
28385238	7	33	theme	biomaterial	1327:1337	arg1	scaffold					1339:1346	useful biomaterial scaffold	1320:1346	useful biomaterial scaffold for cartilage tissue regeneration	1320:1380	Overall, the novel injectable multicomponent hydrogel presented here is expected to be useful biomaterial scaffold for cartilage tissue regeneration.
28385238	0	34	theme	acid/RGD-functionalized	44:66	arg1	hydrogel					75:82	injectable hyaluronic acid/RGD-functionalized pectin hydrogel	22:82	injectable hyaluronic acid/RGD-functionalized pectin hydrogel	22:82	Self-crosslinking and injectable hyaluronic acid/RGD-functionalized pectin hydrogel for cartilage tissue engineering.
28385238	1	35	theme	hydrazone	356:364	arg1	crosslinking					366:377	covalent hydrazone crosslinking	347:377	covalent hydrazone crosslinking of polysaccharides	347:396	In the present study, we developed a biomimetic injectable hydrogel system based on hyaluronic acid-adipic dihydrazide and the oligopeptide G4RGDS-grafted oxidized pectin, in which their hydrazide and aldehyde-derivatives enable covalent hydrazone crosslinking of polysaccharides.
28385238	7	36	theme	cartilage	1352:1360	arg1	regeneration					1369:1380	cartilage tissue regeneration	1352:1380	cartilage tissue regeneration	1352:1380	Overall, the novel injectable multicomponent hydrogel presented here is expected to be useful biomaterial scaffold for cartilage tissue regeneration.
28385238	5	37	theme	oligopeptide	941:952	arg1	amount					924:929	a certain amount	914:929	a certain amount of G4RGDS oligopeptide	914:952	Specifically, the incorporation of a certain amount of G4RGDS oligopeptide into HA/pectin-based hydrogels could serve as a biologically active microenvironment that supported chondrocyte phenotype and facilitated chondrogenesis.
28385238	5	37	theme	oligopeptide	941:952	arg1	oligopeptide					941:952	G4RGDS oligopeptide	934:952	G4RGDS oligopeptide	934:952	Specifically, the incorporation of a certain amount of G4RGDS oligopeptide into HA/pectin-based hydrogels could serve as a biologically active microenvironment that supported chondrocyte phenotype and facilitated chondrogenesis.
28385238	1	38	theme	oligopeptide	245:256	arg1	pectin					282:287	the oligopeptide G4RGDS-grafted oxidized pectin	241:287	the oligopeptide G4RGDS-grafted oxidized pectin	241:287	In the present study, we developed a biomimetic injectable hydrogel system based on hyaluronic acid-adipic dihydrazide and the oligopeptide G4RGDS-grafted oxidized pectin, in which their hydrazide and aldehyde-derivatives enable covalent hydrazone crosslinking of polysaccharides.
28385238	5	39	theme	certain	916:922	arg1	amount					924:929	a certain amount	914:929	a certain amount of G4RGDS oligopeptide	914:952	Specifically, the incorporation of a certain amount of G4RGDS oligopeptide into HA/pectin-based hydrogels could serve as a biologically active microenvironment that supported chondrocyte phenotype and facilitated chondrogenesis.
28385238	5	39	theme	certain	916:922	arg1	oligopeptide					941:952	G4RGDS oligopeptide	934:952	G4RGDS oligopeptide	934:952	Specifically, the incorporation of a certain amount of G4RGDS oligopeptide into HA/pectin-based hydrogels could serve as a biologically active microenvironment that supported chondrocyte phenotype and facilitated chondrogenesis.
28385238	3	40	theme	hydrogel	676:683	arg1	system					685:690	the mechanically stable hydrogel system	652:690	the mechanically stable hydrogel system	652:690	By varying their weight ratios, the physicochemical properties of the mechanically stable hydrogel system were easily adjustable.
28385238	2	41	theme	crosslinking	413:424	arg1	simple					438:443	simple	438:443	simple	438:443	The hydrazone crosslinking strategy is simple, while circumventing toxicity, making this injectable system feasible, minimally invasive and easily translatable for regenerative purposes.
28385238	2	41	theme	crosslinking	413:424	arg1	strategy					426:433	The hydrazone crosslinking strategy	399:433	The hydrazone crosslinking strategy	399:433	The hydrazone crosslinking strategy is simple, while circumventing toxicity, making this injectable system feasible, minimally invasive and easily translatable for regenerative purposes.
28385238	5	42	theme	amount	924:929	arg1	microenvironment					1022:1037	a biologically active microenvironment	1000:1037	a biologically active microenvironment that supported chondrocyte phenotype and facilitated chondrogenesis	1000:1105	Specifically, the incorporation of a certain amount of G4RGDS oligopeptide into HA/pectin-based hydrogels could serve as a biologically active microenvironment that supported chondrocyte phenotype and facilitated chondrogenesis.
28385238	5	42	theme	amount	924:929	arg1	incorporation					897:909	the incorporation	893:909	the incorporation of a certain amount of G4RGDS oligopeptide into HA/pectin-based hydrogels	893:983	Specifically, the incorporation of a certain amount of G4RGDS oligopeptide into HA/pectin-based hydrogels could serve as a biologically active microenvironment that supported chondrocyte phenotype and facilitated chondrogenesis.
28385238	3	43	theme	system	685:690	arg1	properties					638:647	the physicochemical properties	618:647	the physicochemical properties of the mechanically stable hydrogel system	618:690	By varying their weight ratios, the physicochemical properties of the mechanically stable hydrogel system were easily adjustable.
28385238	3	43	theme	system	685:690	arg1	adjustable					704:713	adjustable	704:713	adjustable	704:713	By varying their weight ratios, the physicochemical properties of the mechanically stable hydrogel system were easily adjustable.
28385238	2	44	theme	hydrazone	403:411	arg1	simple					438:443	simple	438:443	simple	438:443	The hydrazone crosslinking strategy is simple, while circumventing toxicity, making this injectable system feasible, minimally invasive and easily translatable for regenerative purposes.
28385238	2	44	theme	hydrazone	403:411	arg1	strategy					426:433	The hydrazone crosslinking strategy	399:433	The hydrazone crosslinking strategy	399:433	The hydrazone crosslinking strategy is simple, while circumventing toxicity, making this injectable system feasible, minimally invasive and easily translatable for regenerative purposes.
28385238	1	45	theme	polysaccharides	382:396	arg1	crosslinking					366:377	covalent hydrazone crosslinking	347:377	covalent hydrazone crosslinking of polysaccharides	347:396	In the present study, we developed a biomimetic injectable hydrogel system based on hyaluronic acid-adipic dihydrazide and the oligopeptide G4RGDS-grafted oxidized pectin, in which their hydrazide and aldehyde-derivatives enable covalent hydrazone crosslinking of polysaccharides.
28385238	1	46	theme	G4RGDS-grafted	258:271	arg1	pectin					282:287	the oligopeptide G4RGDS-grafted oxidized pectin	241:287	the oligopeptide G4RGDS-grafted oxidized pectin	241:287	In the present study, we developed a biomimetic injectable hydrogel system based on hyaluronic acid-adipic dihydrazide and the oligopeptide G4RGDS-grafted oxidized pectin, in which their hydrazide and aldehyde-derivatives enable covalent hydrazone crosslinking of polysaccharides.
28385238	1	47	theme	oxidized	273:280	arg1	pectin					282:287	the oligopeptide G4RGDS-grafted oxidized pectin	241:287	the oligopeptide G4RGDS-grafted oxidized pectin	241:287	In the present study, we developed a biomimetic injectable hydrogel system based on hyaluronic acid-adipic dihydrazide and the oligopeptide G4RGDS-grafted oxidized pectin, in which their hydrazide and aldehyde-derivatives enable covalent hydrazone crosslinking of polysaccharides.
28385238	0	48	theme	tissue	98:103	arg1	engineering					105:115	cartilage tissue engineering	88:115	cartilage tissue engineering	88:115	Self-crosslinking and injectable hyaluronic acid/RGD-functionalized pectin hydrogel for cartilage tissue engineering.
28385238	1	49	dep	which	293:297	arg1	in					290:291	in	290:291	in	290:291	In the present study, we developed a biomimetic injectable hydrogel system based on hyaluronic acid-adipic dihydrazide and the oligopeptide G4RGDS-grafted oxidized pectin, in which their hydrazide and aldehyde-derivatives enable covalent hydrazone crosslinking of polysaccharides.
28385238	1	50	dep	dihydrazide	225:235	arg1	enable					340:345	enable	340:345	enable covalent hydrazone crosslinking of polysaccharides	340:396	In the present study, we developed a biomimetic injectable hydrogel system based on hyaluronic acid-adipic dihydrazide and the oligopeptide G4RGDS-grafted oxidized pectin, in which their hydrazide and aldehyde-derivatives enable covalent hydrazone crosslinking of polysaccharides.
28385238	5	51	theme	HA/pectin-based	959:973	arg1	hydrogels					975:983	HA/pectin-based hydrogels	959:983	HA/pectin-based hydrogels	959:983	Specifically, the incorporation of a certain amount of G4RGDS oligopeptide into HA/pectin-based hydrogels could serve as a biologically active microenvironment that supported chondrocyte phenotype and facilitated chondrogenesis.
28385238	1	52	theme	biomimetic	155:164	arg1	system					186:191	a biomimetic injectable hydrogel system	153:191	a biomimetic injectable hydrogel system based on hyaluronic acid-adipic dihydrazide and the oligopeptide G4RGDS-grafted oxidized pectin, in which their hydrazide and aldehyde-derivatives enable covalent hydrazone crosslinking of polysaccharides	153:396	In the present study, we developed a biomimetic injectable hydrogel system based on hyaluronic acid-adipic dihydrazide and the oligopeptide G4RGDS-grafted oxidized pectin, in which their hydrazide and aldehyde-derivatives enable covalent hydrazone crosslinking of polysaccharides.
28385238	6	53	theme	mouse	1194:1198	arg1	model					1226:1230	a mouse subcutaneous implantation model	1192:1230	a mouse subcutaneous implantation model	1192:1230	Furthermore, the hydrogel system exhibited acceptable tissue compatibility by using a mouse subcutaneous implantation model.
28385238	0	54	theme	cartilage	88:96	arg1	engineering					105:115	cartilage tissue engineering	88:115	cartilage tissue engineering	88:115	Self-crosslinking and injectable hyaluronic acid/RGD-functionalized pectin hydrogel for cartilage tissue engineering.
28385238	2	55	theme	feasible	506:513	arg1	system					499:504	this injectable system	483:504	this injectable system feasible, minimally invasive	483:533	The hydrazone crosslinking strategy is simple, while circumventing toxicity, making this injectable system feasible, minimally invasive and easily translatable for regenerative purposes.
28385238	1	56	theme	injectable	166:175	arg1	system					186:191	a biomimetic injectable hydrogel system	153:191	a biomimetic injectable hydrogel system based on hyaluronic acid-adipic dihydrazide and the oligopeptide G4RGDS-grafted oxidized pectin, in which their hydrazide and aldehyde-derivatives enable covalent hydrazone crosslinking of polysaccharides	153:396	In the present study, we developed a biomimetic injectable hydrogel system based on hyaluronic acid-adipic dihydrazide and the oligopeptide G4RGDS-grafted oxidized pectin, in which their hydrazide and aldehyde-derivatives enable covalent hydrazone crosslinking of polysaccharides.
26731606	5	0	theme	wall-associated	1208:1222	arg1	kinases					1258:1264	two cell wall-associated leucine-rich repeat receptor-like kinases	1199:1264	two cell wall-associated leucine-rich repeat receptor-like kinases	1199:1264	These mutants phenocopy a salt-overly sensitive mutant called sos5, which lacks a fasciclin-like AGP (SOS5/FLA4) as well as a fei1fei2 double mutant, which lacks two cell wall-associated leucine-rich repeat receptor-like kinases.
26731606	1	1	theme	dynamic	316:322	arg1	structure					324:332	a dynamic structure	314:332	a dynamic structure that changes in response to both developmental and environmental cues	314:402	Fundamental processes that underpin plant growth and development depend crucially on the action and assembly of the cell wall, a dynamic structure that changes in response to both developmental and environmental cues.
26731606	1	1	theme	dynamic	316:322	arg1	action					276:281	action	276:281	action	276:281	Fundamental processes that underpin plant growth and development depend crucially on the action and assembly of the cell wall, a dynamic structure that changes in response to both developmental and environmental cues.
26731606	2	2	theme	potential	578:586	arg1	roles					588:592	their potential roles	572:592	their potential roles in cellular signaling	572:614	While much is known about cell wall structure and biosynthesis, much less is known about the functions of the individual wall components, particularly with respect to their potential roles in cellular signaling.
26731606	6	3	theme	reduced	1323:1329	arg1	adherence					1345:1353	reduced seed mucilage adherence	1323:1353	reduced seed mucilage adherence	1323:1353	Additionally, galt2gal5 as well as sos5 and fei2 showed reduced seed mucilage adherence.
26731606	4	4	theme	root	945:948	arg1	growth					934:939	impaired root growth	920:939	impaired root growth	920:939	Notably, galt2galt5 double mutants displayed impaired root growth and root tip swelling in response to salt, likely as a result of decreased cellulose synthesis.
26731606	4	4	theme	root	945:948	arg1	swelling					954:961	root tip swelling	945:961	root tip swelling	945:961	Notably, galt2galt5 double mutants displayed impaired root growth and root tip swelling in response to salt, likely as a result of decreased cellulose synthesis.
26731606	4	4	theme	root	945:948	arg1	result					996:1001	a result	994:1001	a result of decreased cellulose synthesis	994:1034	Notably, galt2galt5 double mutants displayed impaired root growth and root tip swelling in response to salt, likely as a result of decreased cellulose synthesis.
26731606	1	5	dep	action	276:281	arg1	the					272:274	the	272:274	the	272:274	Fundamental processes that underpin plant growth and development depend crucially on the action and assembly of the cell wall, a dynamic structure that changes in response to both developmental and environmental cues.
26731606	4	6	theme	galt2galt5	884:893	arg1	mutants					902:908	galt2galt5 double mutants	884:908	galt2galt5 double mutants	884:908	Notably, galt2galt5 double mutants displayed impaired root growth and root tip swelling in response to salt, likely as a result of decreased cellulose synthesis.
26731606	3	7	theme	Loss-of-function	617:632	arg1	mutants					634:640	Loss-of-function mutants	617:640	Loss-of-function mutants of two arabinogalactan-protein (AGP)-specific galactosyltransferases namely	617:716	Loss-of-function mutants of two arabinogalactan-protein (AGP)-specific galactosyltransferases namely, GALT2 and GALT5, confer pleiotropic growth and development phenotypes indicating the important contributions of carbohydrate moieties towards AGP function.
26731606	3	7	theme	Loss-of-function	617:632	arg1	GALT2					719:723	GALT2	719:723	GALT2	719:723	Loss-of-function mutants of two arabinogalactan-protein (AGP)-specific galactosyltransferases namely, GALT2 and GALT5, confer pleiotropic growth and development phenotypes indicating the important contributions of carbohydrate moieties towards AGP function.
26731606	3	7	theme	Loss-of-function	617:632	arg1	GALT5					729:733	GALT5	729:733	GALT5	729:733	Loss-of-function mutants of two arabinogalactan-protein (AGP)-specific galactosyltransferases namely, GALT2 and GALT5, confer pleiotropic growth and development phenotypes indicating the important contributions of carbohydrate moieties towards AGP function.
26731606	3	8	theme	AGP	861:863	arg1	function					865:872	AGP function	861:872	AGP function	861:872	Loss-of-function mutants of two arabinogalactan-protein (AGP)-specific galactosyltransferases namely, GALT2 and GALT5, confer pleiotropic growth and development phenotypes indicating the important contributions of carbohydrate moieties towards AGP function.
26731606	0	9	theme	Mucilage	95:102	arg1	Adherence					104:112	Seed Mucilage Adherence	90:112	Seed Mucilage Adherence	90:112	Glycosylation of a Fasciclin-Like Arabinogalactan-Protein (SOS5) Mediates Root Growth and Seed Mucilage Adherence via a Cell Wall Receptor-Like Kinase (FEI1/FEI2) Pathway in Arabidopsis.
26731606	3	10	theme	-specific	678:686	arg1	galactosyltransferases					688:709	two arabinogalactan-protein (AGP)-specific galactosyltransferases	645:709	two arabinogalactan-protein (AGP)-specific galactosyltransferases namely	645:716	Loss-of-function mutants of two arabinogalactan-protein (AGP)-specific galactosyltransferases namely, GALT2 and GALT5, confer pleiotropic growth and development phenotypes indicating the important contributions of carbohydrate moieties towards AGP function.
26731606	8	11	theme	quintuple	1539:1547	arg1	mutant					1549:1554	the quintuple mutant	1535:1554	the quintuple mutant	1535:1554	Further genetic and biochemical analysis of the quintuple mutant demonstrated involvement of these genes with the interplay between cellulose biosynthesis and two plant growth regulators, ethylene and ABA, in modulating root cell wall integrity.
26731606	8	12	theme	biochemical	1511:1521	arg1	analysis					1523:1530	Further genetic and biochemical analysis	1491:1530	Further genetic and biochemical analysis of the quintuple mutant	1491:1554	Further genetic and biochemical analysis of the quintuple mutant demonstrated involvement of these genes with the interplay between cellulose biosynthesis and two plant growth regulators, ethylene and ABA, in modulating root cell wall integrity.
26731606	7	13	theme	single	1459:1464	arg1	pathway					1482:1488	a single, linear genetic pathway	1457:1488	a single, linear genetic pathway	1457:1488	Quintuple galt2galt5sos5fei1fei2 mutants were produced and provided evidence that these genes act in a single, linear genetic pathway.
26731606	8	14	theme	wall	1721:1724	arg1	integrity					1726:1734	root cell wall integrity	1711:1734	root cell wall integrity	1711:1734	Further genetic and biochemical analysis of the quintuple mutant demonstrated involvement of these genes with the interplay between cellulose biosynthesis and two plant growth regulators, ethylene and ABA, in modulating root cell wall integrity.
26731606	3	15	theme	carbohydrate	831:842	arg1	moieties					844:851	carbohydrate moieties	831:851	carbohydrate moieties	831:851	Loss-of-function mutants of two arabinogalactan-protein (AGP)-specific galactosyltransferases namely, GALT2 and GALT5, confer pleiotropic growth and development phenotypes indicating the important contributions of carbohydrate moieties towards AGP function.
26731606	0	16	theme	Seed	90:93	arg1	Adherence					104:112	Seed Mucilage Adherence	90:112	Seed Mucilage Adherence	90:112	Glycosylation of a Fasciclin-Like Arabinogalactan-Protein (SOS5) Mediates Root Growth and Seed Mucilage Adherence via a Cell Wall Receptor-Like Kinase (FEI1/FEI2) Pathway in Arabidopsis.
26731606	3	17	theme	moieties	844:851	arg1	contributions					814:826	the important contributions	800:826	the important contributions of carbohydrate moieties towards AGP function	800:872	Loss-of-function mutants of two arabinogalactan-protein (AGP)-specific galactosyltransferases namely, GALT2 and GALT5, confer pleiotropic growth and development phenotypes indicating the important contributions of carbohydrate moieties towards AGP function.
26731606	8	18	theme	mutant	1549:1554	arg1	analysis					1523:1530	Further genetic and biochemical analysis	1491:1530	Further genetic and biochemical analysis of the quintuple mutant	1491:1554	Further genetic and biochemical analysis of the quintuple mutant demonstrated involvement of these genes with the interplay between cellulose biosynthesis and two plant growth regulators, ethylene and ABA, in modulating root cell wall integrity.
26731606	8	19	theme	root	1711:1714	arg1	integrity					1726:1734	root cell wall integrity	1711:1734	root cell wall integrity	1711:1734	Further genetic and biochemical analysis of the quintuple mutant demonstrated involvement of these genes with the interplay between cellulose biosynthesis and two plant growth regulators, ethylene and ABA, in modulating root cell wall integrity.
26731606	7	20	theme	Quintuple	1356:1364	arg1	mutants					1389:1395	Quintuple galt2galt5sos5fei1fei2 mutants	1356:1395	Quintuple galt2galt5sos5fei1fei2 mutants	1356:1395	Quintuple galt2galt5sos5fei1fei2 mutants were produced and provided evidence that these genes act in a single, linear genetic pathway.
26731606	4	21	theme	double	895:900	arg1	mutants					902:908	galt2galt5 double mutants	884:908	galt2galt5 double mutants	884:908	Notably, galt2galt5 double mutants displayed impaired root growth and root tip swelling in response to salt, likely as a result of decreased cellulose synthesis.
26731606	4	22	theme	cellulose	1016:1024	arg1	synthesis					1026:1034	decreased cellulose synthesis	1006:1034	decreased cellulose synthesis	1006:1034	Notably, galt2galt5 double mutants displayed impaired root growth and root tip swelling in response to salt, likely as a result of decreased cellulose synthesis.
26731606	5	23	theme	fasciclin-like	1119:1132	arg1	SOS5/FLA4					1139:1147	SOS5/FLA4	1139:1147	SOS5/FLA4	1139:1147	These mutants phenocopy a salt-overly sensitive mutant called sos5, which lacks a fasciclin-like AGP (SOS5/FLA4) as well as a fei1fei2 double mutant, which lacks two cell wall-associated leucine-rich repeat receptor-like kinases.
26731606	5	23	theme	fasciclin-like	1119:1132	arg1	AGP					1134:1136	a fasciclin-like AGP	1117:1136	a fasciclin-like AGP (SOS5/FLA4) as well as a fei1fei2 double mutant, which lacks two cell wall-associated leucine-rich repeat receptor-like kinases	1117:1264	These mutants phenocopy a salt-overly sensitive mutant called sos5, which lacks a fasciclin-like AGP (SOS5/FLA4) as well as a fei1fei2 double mutant, which lacks two cell wall-associated leucine-rich repeat receptor-like kinases.
26731606	2	24	theme	cellular	597:604	arg1	signaling					606:614	cellular signaling	597:614	cellular signaling	597:614	While much is known about cell wall structure and biosynthesis, much less is known about the functions of the individual wall components, particularly with respect to their potential roles in cellular signaling.
26731606	8	25	theme	plant	1654:1658	arg1	ABA					1692:1694	ABA	1692:1694	ABA	1692:1694	Further genetic and biochemical analysis of the quintuple mutant demonstrated involvement of these genes with the interplay between cellulose biosynthesis and two plant growth regulators, ethylene and ABA, in modulating root cell wall integrity.
26731606	8	25	theme	plant	1654:1658	arg1	ethylene					1679:1686	ethylene	1679:1686	ethylene	1679:1686	Further genetic and biochemical analysis of the quintuple mutant demonstrated involvement of these genes with the interplay between cellulose biosynthesis and two plant growth regulators, ethylene and ABA, in modulating root cell wall integrity.
26731606	8	25	theme	plant	1654:1658	arg1	regulators					1667:1676	two plant growth regulators	1650:1676	two plant growth regulators	1650:1676	Further genetic and biochemical analysis of the quintuple mutant demonstrated involvement of these genes with the interplay between cellulose biosynthesis and two plant growth regulators, ethylene and ABA, in modulating root cell wall integrity.
26731606	2	26	theme	cell	431:434	arg1	structure					441:449	cell wall structure	431:449	cell wall structure	431:449	While much is known about cell wall structure and biosynthesis, much less is known about the functions of the individual wall components, particularly with respect to their potential roles in cellular signaling.
26731606	7	27	theme	genetic	1474:1480	arg1	pathway					1482:1488	a single, linear genetic pathway	1457:1488	a single, linear genetic pathway	1457:1488	Quintuple galt2galt5sos5fei1fei2 mutants were produced and provided evidence that these genes act in a single, linear genetic pathway.
26731606	5	28	theme	double	1172:1177	arg1	mutant					1179:1184	a fei1fei2 double mutant	1161:1184	a fei1fei2 double mutant	1161:1184	These mutants phenocopy a salt-overly sensitive mutant called sos5, which lacks a fasciclin-like AGP (SOS5/FLA4) as well as a fei1fei2 double mutant, which lacks two cell wall-associated leucine-rich repeat receptor-like kinases.
26731606	0	29	theme	Kinase	144:149	arg1	Pathway					163:169	a Cell Wall Receptor-Like Kinase (FEI1/FEI2) Pathway	118:169	a Cell Wall Receptor-Like Kinase (FEI1/FEI2) Pathway in Arabidopsis	118:184	Glycosylation of a Fasciclin-Like Arabinogalactan-Protein (SOS5) Mediates Root Growth and Seed Mucilage Adherence via a Cell Wall Receptor-Like Kinase (FEI1/FEI2) Pathway in Arabidopsis.
26731606	6	30	theme	mucilage	1336:1343	arg1	adherence					1345:1353	reduced seed mucilage adherence	1323:1353	reduced seed mucilage adherence	1323:1353	Additionally, galt2gal5 as well as sos5 and fei2 showed reduced seed mucilage adherence.
26731606	3	31	theme	pleiotropic	743:753	arg1	phenotypes					778:787	pleiotropic growth and development phenotypes	743:787	pleiotropic growth and development phenotypes indicating the important contributions of carbohydrate moieties towards AGP function	743:872	Loss-of-function mutants of two arabinogalactan-protein (AGP)-specific galactosyltransferases namely, GALT2 and GALT5, confer pleiotropic growth and development phenotypes indicating the important contributions of carbohydrate moieties towards AGP function.
26731606	0	32	theme	Fasciclin-Like	19:32	arg1	SOS5					59:62	SOS5	59:62	SOS5	59:62	Glycosylation of a Fasciclin-Like Arabinogalactan-Protein (SOS5) Mediates Root Growth and Seed Mucilage Adherence via a Cell Wall Receptor-Like Kinase (FEI1/FEI2) Pathway in Arabidopsis.
26731606	0	32	theme	Fasciclin-Like	19:32	arg1	Arabinogalactan-Protein					34:56	a Fasciclin-Like Arabinogalactan-Protein	17:56	a Fasciclin-Like Arabinogalactan-Protein (SOS5)	17:63	Glycosylation of a Fasciclin-Like Arabinogalactan-Protein (SOS5) Mediates Root Growth and Seed Mucilage Adherence via a Cell Wall Receptor-Like Kinase (FEI1/FEI2) Pathway in Arabidopsis.
26731606	3	33	theme	growth	755:760	arg1	phenotypes					778:787	pleiotropic growth and development phenotypes	743:787	pleiotropic growth and development phenotypes indicating the important contributions of carbohydrate moieties towards AGP function	743:872	Loss-of-function mutants of two arabinogalactan-protein (AGP)-specific galactosyltransferases namely, GALT2 and GALT5, confer pleiotropic growth and development phenotypes indicating the important contributions of carbohydrate moieties towards AGP function.
26731606	2	34	theme	wall	526:529	arg1	components					531:540	the individual wall components	511:540	the individual wall components	511:540	While much is known about cell wall structure and biosynthesis, much less is known about the functions of the individual wall components, particularly with respect to their potential roles in cellular signaling.
26731606	7	35	theme	galt2galt5sos5fei1fei2	1366:1387	arg1	mutants					1389:1395	Quintuple galt2galt5sos5fei1fei2 mutants	1356:1395	Quintuple galt2galt5sos5fei1fei2 mutants	1356:1395	Quintuple galt2galt5sos5fei1fei2 mutants were produced and provided evidence that these genes act in a single, linear genetic pathway.
26731606	1	36	theme	Fundamental	187:197	arg1	processes					199:207	Fundamental processes	187:207	Fundamental processes that underpin plant growth and development	187:250	Fundamental processes that underpin plant growth and development depend crucially on the action and assembly of the cell wall, a dynamic structure that changes in response to both developmental and environmental cues.
26731606	2	37	theme	individual	515:524	arg1	components					531:540	the individual wall components	511:540	the individual wall components	511:540	While much is known about cell wall structure and biosynthesis, much less is known about the functions of the individual wall components, particularly with respect to their potential roles in cellular signaling.
26731606	4	38	theme	decreased	1006:1014	arg1	synthesis					1026:1034	decreased cellulose synthesis	1006:1034	decreased cellulose synthesis	1006:1034	Notably, galt2galt5 double mutants displayed impaired root growth and root tip swelling in response to salt, likely as a result of decreased cellulose synthesis.
26731606	0	39	from	Pathway	163:169	arg1	Arabidopsis					174:184	Arabidopsis	174:184	Arabidopsis	174:184	Glycosylation of a Fasciclin-Like Arabinogalactan-Protein (SOS5) Mediates Root Growth and Seed Mucilage Adherence via a Cell Wall Receptor-Like Kinase (FEI1/FEI2) Pathway in Arabidopsis.
26731606	0	40	theme	Receptor-Like	130:142	arg1	FEI1/FEI2					152:160	FEI1/FEI2	152:160	FEI1/FEI2	152:160	Glycosylation of a Fasciclin-Like Arabinogalactan-Protein (SOS5) Mediates Root Growth and Seed Mucilage Adherence via a Cell Wall Receptor-Like Kinase (FEI1/FEI2) Pathway in Arabidopsis.
26731606	0	40	theme	Receptor-Like	130:142	arg1	Kinase					144:149	Cell Wall Receptor-Like Kinase	120:149	a Cell Wall Receptor-Like Kinase (FEI1/FEI2) Pathway in Arabidopsis	118:184	Glycosylation of a Fasciclin-Like Arabinogalactan-Protein (SOS5) Mediates Root Growth and Seed Mucilage Adherence via a Cell Wall Receptor-Like Kinase (FEI1/FEI2) Pathway in Arabidopsis.
26731606	1	41	theme	developmental	367:379	arg1	cues					399:402	both developmental and environmental cues	362:402	both developmental and environmental cues	362:402	Fundamental processes that underpin plant growth and development depend crucially on the action and assembly of the cell wall, a dynamic structure that changes in response to both developmental and environmental cues.
26731606	0	42	gly	Glycosylation	0:12	arg1	SOS5					59:62	SOS5	59:62	SOS5	59:62	Glycosylation of a Fasciclin-Like Arabinogalactan-Protein (SOS5) Mediates Root Growth and Seed Mucilage Adherence via a Cell Wall Receptor-Like Kinase (FEI1/FEI2) Pathway in Arabidopsis.
26731606	0	42	gly	Glycosylation	0:12	arg1	Arabinogalactan-Protein					34:56	a Fasciclin-Like Arabinogalactan-Protein	17:56	a Fasciclin-Like Arabinogalactan-Protein (SOS5)	17:63	Glycosylation of a Fasciclin-Like Arabinogalactan-Protein (SOS5) Mediates Root Growth and Seed Mucilage Adherence via a Cell Wall Receptor-Like Kinase (FEI1/FEI2) Pathway in Arabidopsis.
26731606	0	43	theme	Arabinogalactan-Protein	34:56	arg1	Glycosylation					0:12	Glycosylation	0:12	Glycosylation of a Fasciclin-Like Arabinogalactan-Protein (SOS5)	0:63	Glycosylation of a Fasciclin-Like Arabinogalactan-Protein (SOS5) Mediates Root Growth and Seed Mucilage Adherence via a Cell Wall Receptor-Like Kinase (FEI1/FEI2) Pathway in Arabidopsis.
26731606	0	44	theme	Wall	125:128	arg1	FEI1/FEI2					152:160	FEI1/FEI2	152:160	FEI1/FEI2	152:160	Glycosylation of a Fasciclin-Like Arabinogalactan-Protein (SOS5) Mediates Root Growth and Seed Mucilage Adherence via a Cell Wall Receptor-Like Kinase (FEI1/FEI2) Pathway in Arabidopsis.
26731606	0	44	theme	Wall	125:128	arg1	Kinase					144:149	Cell Wall Receptor-Like Kinase	120:149	a Cell Wall Receptor-Like Kinase (FEI1/FEI2) Pathway in Arabidopsis	118:184	Glycosylation of a Fasciclin-Like Arabinogalactan-Protein (SOS5) Mediates Root Growth and Seed Mucilage Adherence via a Cell Wall Receptor-Like Kinase (FEI1/FEI2) Pathway in Arabidopsis.
26731606	8	45	theme	growth	1660:1665	arg1	ABA					1692:1694	ABA	1692:1694	ABA	1692:1694	Further genetic and biochemical analysis of the quintuple mutant demonstrated involvement of these genes with the interplay between cellulose biosynthesis and two plant growth regulators, ethylene and ABA, in modulating root cell wall integrity.
26731606	8	45	theme	growth	1660:1665	arg1	ethylene					1679:1686	ethylene	1679:1686	ethylene	1679:1686	Further genetic and biochemical analysis of the quintuple mutant demonstrated involvement of these genes with the interplay between cellulose biosynthesis and two plant growth regulators, ethylene and ABA, in modulating root cell wall integrity.
26731606	8	45	theme	growth	1660:1665	arg1	regulators					1667:1676	two plant growth regulators	1650:1676	two plant growth regulators	1650:1676	Further genetic and biochemical analysis of the quintuple mutant demonstrated involvement of these genes with the interplay between cellulose biosynthesis and two plant growth regulators, ethylene and ABA, in modulating root cell wall integrity.
26731606	7	46	dep	single	1459:1464	arg1	linear					1467:1472	linear	1467:1472	linear	1467:1472	Quintuple galt2galt5sos5fei1fei2 mutants were produced and provided evidence that these genes act in a single, linear genetic pathway.
26731606	5	47	theme	fei1fei2	1163:1170	arg1	mutant					1179:1184	a fei1fei2 double mutant	1161:1184	a fei1fei2 double mutant	1161:1184	These mutants phenocopy a salt-overly sensitive mutant called sos5, which lacks a fasciclin-like AGP (SOS5/FLA4) as well as a fei1fei2 double mutant, which lacks two cell wall-associated leucine-rich repeat receptor-like kinases.
26731606	4	48	theme	synthesis	1026:1034	arg1	growth					934:939	impaired root growth	920:939	impaired root growth	920:939	Notably, galt2galt5 double mutants displayed impaired root growth and root tip swelling in response to salt, likely as a result of decreased cellulose synthesis.
26731606	4	48	theme	synthesis	1026:1034	arg1	swelling					954:961	root tip swelling	945:961	root tip swelling	945:961	Notably, galt2galt5 double mutants displayed impaired root growth and root tip swelling in response to salt, likely as a result of decreased cellulose synthesis.
26731606	4	48	theme	synthesis	1026:1034	arg1	result					996:1001	a result	994:1001	a result of decreased cellulose synthesis	994:1034	Notably, galt2galt5 double mutants displayed impaired root growth and root tip swelling in response to salt, likely as a result of decreased cellulose synthesis.
26731606	1	49	theme	cell	303:306	arg1	wall					308:311	the cell wall	299:311	the cell wall	299:311	Fundamental processes that underpin plant growth and development depend crucially on the action and assembly of the cell wall, a dynamic structure that changes in response to both developmental and environmental cues.
26731606	4	50	theme	root	929:932	arg1	growth					934:939	impaired root growth	920:939	impaired root growth	920:939	Notably, galt2galt5 double mutants displayed impaired root growth and root tip swelling in response to salt, likely as a result of decreased cellulose synthesis.
26731606	4	50	theme	root	929:932	arg1	swelling					954:961	root tip swelling	945:961	root tip swelling	945:961	Notably, galt2galt5 double mutants displayed impaired root growth and root tip swelling in response to salt, likely as a result of decreased cellulose synthesis.
26731606	4	50	theme	root	929:932	arg1	result					996:1001	a result	994:1001	a result of decreased cellulose synthesis	994:1034	Notably, galt2galt5 double mutants displayed impaired root growth and root tip swelling in response to salt, likely as a result of decreased cellulose synthesis.
26731606	1	51	theme	environmental	385:397	arg1	cues					399:402	both developmental and environmental cues	362:402	both developmental and environmental cues	362:402	Fundamental processes that underpin plant growth and development depend crucially on the action and assembly of the cell wall, a dynamic structure that changes in response to both developmental and environmental cues.
26731606	2	52	theme	wall	436:439	arg1	structure					441:449	cell wall structure	431:449	cell wall structure	431:449	While much is known about cell wall structure and biosynthesis, much less is known about the functions of the individual wall components, particularly with respect to their potential roles in cellular signaling.
26731606	1	53	theme	wall	308:311	arg1	assembly					287:294	assembly	287:294	assembly	287:294	Fundamental processes that underpin plant growth and development depend crucially on the action and assembly of the cell wall, a dynamic structure that changes in response to both developmental and environmental cues.
26731606	1	53	theme	wall	308:311	arg1	structure					324:332	a dynamic structure	314:332	a dynamic structure that changes in response to both developmental and environmental cues	314:402	Fundamental processes that underpin plant growth and development depend crucially on the action and assembly of the cell wall, a dynamic structure that changes in response to both developmental and environmental cues.
26731606	1	53	theme	wall	308:311	arg1	action					276:281	action	276:281	action	276:281	Fundamental processes that underpin plant growth and development depend crucially on the action and assembly of the cell wall, a dynamic structure that changes in response to both developmental and environmental cues.
26731606	0	54	theme	Cell	120:123	arg1	FEI1/FEI2					152:160	FEI1/FEI2	152:160	FEI1/FEI2	152:160	Glycosylation of a Fasciclin-Like Arabinogalactan-Protein (SOS5) Mediates Root Growth and Seed Mucilage Adherence via a Cell Wall Receptor-Like Kinase (FEI1/FEI2) Pathway in Arabidopsis.
26731606	0	54	theme	Cell	120:123	arg1	Kinase					144:149	Cell Wall Receptor-Like Kinase	120:149	a Cell Wall Receptor-Like Kinase (FEI1/FEI2) Pathway in Arabidopsis	118:184	Glycosylation of a Fasciclin-Like Arabinogalactan-Protein (SOS5) Mediates Root Growth and Seed Mucilage Adherence via a Cell Wall Receptor-Like Kinase (FEI1/FEI2) Pathway in Arabidopsis.
26731606	8	55	theme	genetic	1499:1505	arg1	analysis					1523:1530	Further genetic and biochemical analysis	1491:1530	Further genetic and biochemical analysis of the quintuple mutant	1491:1554	Further genetic and biochemical analysis of the quintuple mutant demonstrated involvement of these genes with the interplay between cellulose biosynthesis and two plant growth regulators, ethylene and ABA, in modulating root cell wall integrity.
26731606	8	56	theme	Further	1491:1497	arg1	analysis					1523:1530	Further genetic and biochemical analysis	1491:1530	Further genetic and biochemical analysis of the quintuple mutant	1491:1554	Further genetic and biochemical analysis of the quintuple mutant demonstrated involvement of these genes with the interplay between cellulose biosynthesis and two plant growth regulators, ethylene and ABA, in modulating root cell wall integrity.
26731606	8	57	theme	cellulose	1623:1631	arg1	biosynthesis					1633:1644	cellulose biosynthesis	1623:1644	cellulose biosynthesis	1623:1644	Further genetic and biochemical analysis of the quintuple mutant demonstrated involvement of these genes with the interplay between cellulose biosynthesis and two plant growth regulators, ethylene and ABA, in modulating root cell wall integrity.
26731606	3	58	theme	development	766:776	arg1	phenotypes					778:787	pleiotropic growth and development phenotypes	743:787	pleiotropic growth and development phenotypes indicating the important contributions of carbohydrate moieties towards AGP function	743:872	Loss-of-function mutants of two arabinogalactan-protein (AGP)-specific galactosyltransferases namely, GALT2 and GALT5, confer pleiotropic growth and development phenotypes indicating the important contributions of carbohydrate moieties towards AGP function.
26731606	8	59	theme	genes	1590:1594	arg1	involvement					1569:1579	involvement	1569:1579	involvement of these genes	1569:1594	Further genetic and biochemical analysis of the quintuple mutant demonstrated involvement of these genes with the interplay between cellulose biosynthesis and two plant growth regulators, ethylene and ABA, in modulating root cell wall integrity.
26731606	6	60	theme	seed	1331:1334	arg1	adherence					1345:1353	reduced seed mucilage adherence	1323:1353	reduced seed mucilage adherence	1323:1353	Additionally, galt2gal5 as well as sos5 and fei2 showed reduced seed mucilage adherence.
26731606	1	61	theme	plant	223:227	arg1	growth					229:234	plant growth	223:234	plant growth	223:234	Fundamental processes that underpin plant growth and development depend crucially on the action and assembly of the cell wall, a dynamic structure that changes in response to both developmental and environmental cues.
26731606	2	62	theme	components	531:540	arg1	functions					498:506	the functions	494:506	the functions of the individual wall components	494:540	While much is known about cell wall structure and biosynthesis, much less is known about the functions of the individual wall components, particularly with respect to their potential roles in cellular signaling.
26731606	5	63	theme	sensitive	1075:1083	arg1	mutant					1085:1090	a salt-overly sensitive mutant	1061:1090	a salt-overly sensitive mutant	1061:1090	These mutants phenocopy a salt-overly sensitive mutant called sos5, which lacks a fasciclin-like AGP (SOS5/FLA4) as well as a fei1fei2 double mutant, which lacks two cell wall-associated leucine-rich repeat receptor-like kinases.
26731606	5	64	theme	repeat	1237:1242	arg1	kinases					1258:1264	two cell wall-associated leucine-rich repeat receptor-like kinases	1199:1264	two cell wall-associated leucine-rich repeat receptor-like kinases	1199:1264	These mutants phenocopy a salt-overly sensitive mutant called sos5, which lacks a fasciclin-like AGP (SOS5/FLA4) as well as a fei1fei2 double mutant, which lacks two cell wall-associated leucine-rich repeat receptor-like kinases.
26731606	0	65	theme	Root	74:77	arg1	Growth					79:84	Root Growth	74:84	Root Growth	74:84	Glycosylation of a Fasciclin-Like Arabinogalactan-Protein (SOS5) Mediates Root Growth and Seed Mucilage Adherence via a Cell Wall Receptor-Like Kinase (FEI1/FEI2) Pathway in Arabidopsis.
26731606	4	66	theme	tip	950:952	arg1	growth					934:939	impaired root growth	920:939	impaired root growth	920:939	Notably, galt2galt5 double mutants displayed impaired root growth and root tip swelling in response to salt, likely as a result of decreased cellulose synthesis.
26731606	4	66	theme	tip	950:952	arg1	swelling					954:961	root tip swelling	945:961	root tip swelling	945:961	Notably, galt2galt5 double mutants displayed impaired root growth and root tip swelling in response to salt, likely as a result of decreased cellulose synthesis.
26731606	4	66	theme	tip	950:952	arg1	result					996:1001	a result	994:1001	a result of decreased cellulose synthesis	994:1034	Notably, galt2galt5 double mutants displayed impaired root growth and root tip swelling in response to salt, likely as a result of decreased cellulose synthesis.
26731606	4	67	dep	salt	978:981	arg1	response					966:973	response	966:973	response	966:973	Notably, galt2galt5 double mutants displayed impaired root growth and root tip swelling in response to salt, likely as a result of decreased cellulose synthesis.
26731606	4	68	theme	impaired	920:927	arg1	growth					934:939	impaired root growth	920:939	impaired root growth	920:939	Notably, galt2galt5 double mutants displayed impaired root growth and root tip swelling in response to salt, likely as a result of decreased cellulose synthesis.
26731606	4	68	theme	impaired	920:927	arg1	swelling					954:961	root tip swelling	945:961	root tip swelling	945:961	Notably, galt2galt5 double mutants displayed impaired root growth and root tip swelling in response to salt, likely as a result of decreased cellulose synthesis.
26731606	4	68	theme	impaired	920:927	arg1	result					996:1001	a result	994:1001	a result of decreased cellulose synthesis	994:1034	Notably, galt2galt5 double mutants displayed impaired root growth and root tip swelling in response to salt, likely as a result of decreased cellulose synthesis.
26731606	5	69	theme	receptor-like	1244:1256	arg1	kinases					1258:1264	two cell wall-associated leucine-rich repeat receptor-like kinases	1199:1264	two cell wall-associated leucine-rich repeat receptor-like kinases	1199:1264	These mutants phenocopy a salt-overly sensitive mutant called sos5, which lacks a fasciclin-like AGP (SOS5/FLA4) as well as a fei1fei2 double mutant, which lacks two cell wall-associated leucine-rich repeat receptor-like kinases.
26731606	5	70	theme	cell	1203:1206	arg1	kinases					1258:1264	two cell wall-associated leucine-rich repeat receptor-like kinases	1199:1264	two cell wall-associated leucine-rich repeat receptor-like kinases	1199:1264	These mutants phenocopy a salt-overly sensitive mutant called sos5, which lacks a fasciclin-like AGP (SOS5/FLA4) as well as a fei1fei2 double mutant, which lacks two cell wall-associated leucine-rich repeat receptor-like kinases.
26731606	3	71	theme	galactosyltransferases	688:709	arg1	mutants					634:640	Loss-of-function mutants	617:640	Loss-of-function mutants of two arabinogalactan-protein (AGP)-specific galactosyltransferases namely	617:716	Loss-of-function mutants of two arabinogalactan-protein (AGP)-specific galactosyltransferases namely, GALT2 and GALT5, confer pleiotropic growth and development phenotypes indicating the important contributions of carbohydrate moieties towards AGP function.
26731606	3	71	theme	galactosyltransferases	688:709	arg1	GALT2					719:723	GALT2	719:723	GALT2	719:723	Loss-of-function mutants of two arabinogalactan-protein (AGP)-specific galactosyltransferases namely, GALT2 and GALT5, confer pleiotropic growth and development phenotypes indicating the important contributions of carbohydrate moieties towards AGP function.
26731606	3	71	theme	galactosyltransferases	688:709	arg1	GALT5					729:733	GALT5	729:733	GALT5	729:733	Loss-of-function mutants of two arabinogalactan-protein (AGP)-specific galactosyltransferases namely, GALT2 and GALT5, confer pleiotropic growth and development phenotypes indicating the important contributions of carbohydrate moieties towards AGP function.
26731606	3	72	theme	important	804:812	arg1	contributions					814:826	the important contributions	800:826	the important contributions of carbohydrate moieties towards AGP function	800:872	Loss-of-function mutants of two arabinogalactan-protein (AGP)-specific galactosyltransferases namely, GALT2 and GALT5, confer pleiotropic growth and development phenotypes indicating the important contributions of carbohydrate moieties towards AGP function.
26731606	5	73	theme	leucine-rich	1224:1235	arg1	kinases					1258:1264	two cell wall-associated leucine-rich repeat receptor-like kinases	1199:1264	two cell wall-associated leucine-rich repeat receptor-like kinases	1199:1264	These mutants phenocopy a salt-overly sensitive mutant called sos5, which lacks a fasciclin-like AGP (SOS5/FLA4) as well as a fei1fei2 double mutant, which lacks two cell wall-associated leucine-rich repeat receptor-like kinases.
26731606	8	74	theme	cell	1716:1719	arg1	integrity					1726:1734	root cell wall integrity	1711:1734	root cell wall integrity	1711:1734	Further genetic and biochemical analysis of the quintuple mutant demonstrated involvement of these genes with the interplay between cellulose biosynthesis and two plant growth regulators, ethylene and ABA, in modulating root cell wall integrity.
26731606	2	75	from	roles	588:592	arg1	signaling					606:614	cellular signaling	597:614	cellular signaling	597:614	While much is known about cell wall structure and biosynthesis, much less is known about the functions of the individual wall components, particularly with respect to their potential roles in cellular signaling.
24342348	0	0	theme	groups	86:91	arg1	influence					19:27	the influence	15:27	the influence of C5-hydroxymethyl group and configurations of hydroxyl groups	15:91	Examination of the influence of C5-hydroxymethyl group and configurations of hydroxyl groups at C2, C3, and C4 stereocentres on the N-glycosidic torsion: synthesis and X-ray crystallographic investigation of N-(D-ribopyranosyl)alkanamides as N-glycoprotein linkage region analogs.
24342348	2	1	theme	crystallographic	477:492	arg1	studies					494:500	Earlier crystallographic studies	469:500	Earlier crystallographic studies aimed at understanding the structural significance of the linkage region constituents	469:586	Earlier crystallographic studies aimed at understanding the structural significance of the linkage region constituents revealed that N-glycosidic torsion, ϕN is influenced considerably by variation in the glycan part as compared to the aglycon moiety.
24342348	2	2	theme	region	568:573	arg1	constituents					575:586	the linkage region constituents	556:586	the linkage region constituents	556:586	Earlier crystallographic studies aimed at understanding the structural significance of the linkage region constituents revealed that N-glycosidic torsion, ϕN is influenced considerably by variation in the glycan part as compared to the aglycon moiety.
24342348	6	3	theme	ribosyl	1108:1114	arg1	alkanamides					1116:1126	ribosyl alkanamides	1108:1126	ribosyl alkanamides along with xylosyl and arabinosyl alkanamides	1108:1172	A comprehensive crystal structure analysis of ribosyl alkanamides along with xylosyl and arabinosyl alkanamides showed the wide range of deviations in their ϕN values as compared to the negligible deviation shown by hexopyranosyl alkanamides.
24342348	0	4	theme	hydroxyl	77:84	arg1	groups					86:91	hydroxyl groups	77:91	hydroxyl groups	77:91	Examination of the influence of C5-hydroxymethyl group and configurations of hydroxyl groups at C2, C3, and C4 stereocentres on the N-glycosidic torsion: synthesis and X-ray crystallographic investigation of N-(D-ribopyranosyl)alkanamides as N-glycoprotein linkage region analogs.
24342348	1	5	from	present	316:322	arg1	eukaryotes					327:336	eukaryotes	327:336	eukaryotes	327:336	N-Linked glycosylation is not only present in eukaryotes but also occurs in archaea and bacteria and is mainly characterized by the β-glucosylamine linkage to the asparagine (GlcNAcβAsn).
24342348	2	6	theme	glycan	674:679	arg1	part					681:684	the glycan part	670:684	the glycan part	670:684	Earlier crystallographic studies aimed at understanding the structural significance of the linkage region constituents revealed that N-glycosidic torsion, ϕN is influenced considerably by variation in the glycan part as compared to the aglycon moiety.
24342348	0	7	gly	N-glycoprotein	242:255	arg1	N-glycoprotein					242:255	N-glycoprotein linkage region analogs	242:278	N-glycoprotein linkage region analogs	242:278	Examination of the influence of C5-hydroxymethyl group and configurations of hydroxyl groups at C2, C3, and C4 stereocentres on the N-glycosidic torsion: synthesis and X-ray crystallographic investigation of N-(D-ribopyranosyl)alkanamides as N-glycoprotein linkage region analogs.
24342348	2	8	theme	structural	529:538	arg1	significance					540:551	the structural significance	525:551	the structural significance of the linkage region constituents	525:586	Earlier crystallographic studies aimed at understanding the structural significance of the linkage region constituents revealed that N-glycosidic torsion, ϕN is influenced considerably by variation in the glycan part as compared to the aglycon moiety.
24342348	7	9	theme	C2	1403:1404	arg1	stereocentres					1418:1430	C2, C3, and C4 stereocentres	1403:1430	C2, C3, and C4 stereocentres	1403:1430	This study revealed the importance of C5-hydroxymethyl group and hydroxyl group configurations at C2, C3, and C4 stereocentres in controlling the N-glycosidic torsions.
24342348	6	10	from	values	1222:1227	arg1	range					1190:1194	the wide range	1181:1194	the wide range of deviations in their ϕN values	1181:1227	A comprehensive crystal structure analysis of ribosyl alkanamides along with xylosyl and arabinosyl alkanamides showed the wide range of deviations in their ϕN values as compared to the negligible deviation shown by hexopyranosyl alkanamides.
24342348	6	11	theme	wide	1185:1188	arg1	range					1190:1194	the wide range	1181:1194	the wide range of deviations in their ϕN values	1181:1227	A comprehensive crystal structure analysis of ribosyl alkanamides along with xylosyl and arabinosyl alkanamides showed the wide range of deviations in their ϕN values as compared to the negligible deviation shown by hexopyranosyl alkanamides.
24342348	2	12	theme	N-glycosidic	602:613	arg1	ϕN					624:625	ϕN	624:625	ϕN	624:625	Earlier crystallographic studies aimed at understanding the structural significance of the linkage region constituents revealed that N-glycosidic torsion, ϕN is influenced considerably by variation in the glycan part as compared to the aglycon moiety.
24342348	2	12	theme	N-glycosidic	602:613	arg1	torsion					615:621	N-glycosidic torsion	602:621	N-glycosidic torsion	602:621	Earlier crystallographic studies aimed at understanding the structural significance of the linkage region constituents revealed that N-glycosidic torsion, ϕN is influenced considerably by variation in the glycan part as compared to the aglycon moiety.
24342348	3	13	theme	ϕN	725:726	arg1	value					728:732	The ϕN value	721:732	The ϕN value observed for XylβNHAc	721:754	The ϕN value observed for XylβNHAc deviated maximum as compared to that of the model compound, GlcNAcβNHAc.
24342348	6	14	theme	hexopyranosyl	1278:1290	arg1	alkanamides					1292:1302	hexopyranosyl alkanamides	1278:1302	hexopyranosyl alkanamides	1278:1302	A comprehensive crystal structure analysis of ribosyl alkanamides along with xylosyl and arabinosyl alkanamides showed the wide range of deviations in their ϕN values as compared to the negligible deviation shown by hexopyranosyl alkanamides.
24342348	6	15	from	range	1190:1194	arg1	values					1222:1227	their ϕN values	1213:1227	their ϕN values	1213:1227	A comprehensive crystal structure analysis of ribosyl alkanamides along with xylosyl and arabinosyl alkanamides showed the wide range of deviations in their ϕN values as compared to the negligible deviation shown by hexopyranosyl alkanamides.
24342348	4	16	theme	N-glycosidic	902:913	arg1	torsions					915:922	the N-glycosidic torsions	898:922	the N-glycosidic torsions	898:922	The present work was undertaken to assess the influence of ribose on the N-glycosidic torsions and molecular assembly.
24342348	1	17	from	eukaryotes	327:336	arg1	present					316:322	present	316:322	present	316:322	N-Linked glycosylation is not only present in eukaryotes but also occurs in archaea and bacteria and is mainly characterized by the β-glucosylamine linkage to the asparagine (GlcNAcβAsn).
24342348	0	18	theme	crystallographic	174:189	arg1	investigation					191:203	X-ray crystallographic investigation	168:203	X-ray crystallographic investigation	168:203	Examination of the influence of C5-hydroxymethyl group and configurations of hydroxyl groups at C2, C3, and C4 stereocentres on the N-glycosidic torsion: synthesis and X-ray crystallographic investigation of N-(D-ribopyranosyl)alkanamides as N-glycoprotein linkage region analogs.
24342348	0	19	theme	N-glycoprotein	242:255	arg1	analogs					272:278	N-glycoprotein linkage region analogs	242:278	N-glycoprotein linkage region analogs	242:278	Examination of the influence of C5-hydroxymethyl group and configurations of hydroxyl groups at C2, C3, and C4 stereocentres on the N-glycosidic torsion: synthesis and X-ray crystallographic investigation of N-(D-ribopyranosyl)alkanamides as N-glycoprotein linkage region analogs.
24342348	7	20	theme	N-glycosidic	1451:1462	arg1	torsions					1464:1471	the N-glycosidic torsions	1447:1471	the N-glycosidic torsions	1447:1471	This study revealed the importance of C5-hydroxymethyl group and hydroxyl group configurations at C2, C3, and C4 stereocentres in controlling the N-glycosidic torsions.
24342348	6	21	theme	structure	1086:1094	arg1	analysis					1096:1103	A comprehensive crystal structure analysis	1062:1103	A comprehensive crystal structure analysis of ribosyl alkanamides along with xylosyl and arabinosyl alkanamides	1062:1172	A comprehensive crystal structure analysis of ribosyl alkanamides along with xylosyl and arabinosyl alkanamides showed the wide range of deviations in their ϕN values as compared to the negligible deviation shown by hexopyranosyl alkanamides.
24342348	2	22	theme	Earlier	469:475	arg1	studies					494:500	Earlier crystallographic studies	469:500	Earlier crystallographic studies aimed at understanding the structural significance of the linkage region constituents	469:586	Earlier crystallographic studies aimed at understanding the structural significance of the linkage region constituents revealed that N-glycosidic torsion, ϕN is influenced considerably by variation in the glycan part as compared to the aglycon moiety.
24342348	0	23	theme	N-	208:209	arg1	investigation					191:203	X-ray crystallographic investigation	168:203	X-ray crystallographic investigation	168:203	Examination of the influence of C5-hydroxymethyl group and configurations of hydroxyl groups at C2, C3, and C4 stereocentres on the N-glycosidic torsion: synthesis and X-ray crystallographic investigation of N-(D-ribopyranosyl)alkanamides as N-glycoprotein linkage region analogs.
24342348	0	23	theme	N-	208:209	arg1	synthesis					154:162	synthesis	154:162	synthesis	154:162	Examination of the influence of C5-hydroxymethyl group and configurations of hydroxyl groups at C2, C3, and C4 stereocentres on the N-glycosidic torsion: synthesis and X-ray crystallographic investigation of N-(D-ribopyranosyl)alkanamides as N-glycoprotein linkage region analogs.
24342348	5	24	theme	Several	948:954	arg1	alkanamides					970:980	Several ribopyranosyl alkanamides	948:980	Several ribopyranosyl alkanamides	948:980	Several ribopyranosyl alkanamides have been synthesized and crystal structures of three of them have been solved.
24342348	6	25	from	deviations	1199:1208	arg1	values					1222:1227	their ϕN values	1213:1227	their ϕN values	1213:1227	A comprehensive crystal structure analysis of ribosyl alkanamides along with xylosyl and arabinosyl alkanamides showed the wide range of deviations in their ϕN values as compared to the negligible deviation shown by hexopyranosyl alkanamides.
24342348	6	26	theme	crystal	1078:1084	arg1	analysis					1096:1103	A comprehensive crystal structure analysis	1062:1103	A comprehensive crystal structure analysis of ribosyl alkanamides along with xylosyl and arabinosyl alkanamides	1062:1172	A comprehensive crystal structure analysis of ribosyl alkanamides along with xylosyl and arabinosyl alkanamides showed the wide range of deviations in their ϕN values as compared to the negligible deviation shown by hexopyranosyl alkanamides.
24342348	0	27	theme	region	265:270	arg1	analogs					272:278	N-glycoprotein linkage region analogs	242:278	N-glycoprotein linkage region analogs	242:278	Examination of the influence of C5-hydroxymethyl group and configurations of hydroxyl groups at C2, C3, and C4 stereocentres on the N-glycosidic torsion: synthesis and X-ray crystallographic investigation of N-(D-ribopyranosyl)alkanamides as N-glycoprotein linkage region analogs.
24342348	6	28	theme	comprehensive	1064:1076	arg1	analysis					1096:1103	A comprehensive crystal structure analysis	1062:1103	A comprehensive crystal structure analysis of ribosyl alkanamides along with xylosyl and arabinosyl alkanamides	1062:1172	A comprehensive crystal structure analysis of ribosyl alkanamides along with xylosyl and arabinosyl alkanamides showed the wide range of deviations in their ϕN values as compared to the negligible deviation shown by hexopyranosyl alkanamides.
24342348	0	29	theme	influence	19:27	arg1	Examination					0:10	Examination	0:10	Examination of the influence of C5-hydroxymethyl group and configurations of hydroxyl groups at C2, C3, and C4	0:109	Examination of the influence of C5-hydroxymethyl group and configurations of hydroxyl groups at C2, C3, and C4 stereocentres on the N-glycosidic torsion: synthesis and X-ray crystallographic investigation of N-(D-ribopyranosyl)alkanamides as N-glycoprotein linkage region analogs.
24342348	7	30	theme	group	1379:1383	arg1	importance					1329:1338	the importance	1325:1338	the importance of C5-hydroxymethyl group and hydroxyl group configurations	1325:1398	This study revealed the importance of C5-hydroxymethyl group and hydroxyl group configurations at C2, C3, and C4 stereocentres in controlling the N-glycosidic torsions.
24342348	2	31	theme	linkage	560:566	arg1	constituents					575:586	the linkage region constituents	556:586	the linkage region constituents	556:586	Earlier crystallographic studies aimed at understanding the structural significance of the linkage region constituents revealed that N-glycosidic torsion, ϕN is influenced considerably by variation in the glycan part as compared to the aglycon moiety.
24342348	0	32	theme	linkage	257:263	arg1	analogs					272:278	N-glycoprotein linkage region analogs	242:278	N-glycoprotein linkage region analogs	242:278	Examination of the influence of C5-hydroxymethyl group and configurations of hydroxyl groups at C2, C3, and C4 stereocentres on the N-glycosidic torsion: synthesis and X-ray crystallographic investigation of N-(D-ribopyranosyl)alkanamides as N-glycoprotein linkage region analogs.
24342348	1	33	located	present	316:322	arg2	glycosylation					290:302	N-Linked glycosylation	281:302	N-Linked glycosylation	281:302	N-Linked glycosylation is not only present in eukaryotes but also occurs in archaea and bacteria and is mainly characterized by the β-glucosylamine linkage to the asparagine (GlcNAcβAsn).
24342348	1	33	located	present	316:322	arg1	eukaryotes					327:336	eukaryotes	327:336	eukaryotes	327:336	N-Linked glycosylation is not only present in eukaryotes but also occurs in archaea and bacteria and is mainly characterized by the β-glucosylamine linkage to the asparagine (GlcNAcβAsn).
24342348	6	34	theme	negligible	1248:1257	arg1	deviation					1259:1267	the negligible deviation	1244:1267	the negligible deviation shown by hexopyranosyl alkanamides	1244:1302	A comprehensive crystal structure analysis of ribosyl alkanamides along with xylosyl and arabinosyl alkanamides showed the wide range of deviations in their ϕN values as compared to the negligible deviation shown by hexopyranosyl alkanamides.
24342348	1	35	theme	N-Linked	281:288	arg1	glycosylation					290:302	N-Linked glycosylation	281:302	N-Linked glycosylation	281:302	N-Linked glycosylation is not only present in eukaryotes but also occurs in archaea and bacteria and is mainly characterized by the β-glucosylamine linkage to the asparagine (GlcNAcβAsn).
24342348	2	36	theme	aglycon	705:711	arg1	moiety					713:718	the aglycon moiety	701:718	the aglycon moiety	701:718	Earlier crystallographic studies aimed at understanding the structural significance of the linkage region constituents revealed that N-glycosidic torsion, ϕN is influenced considerably by variation in the glycan part as compared to the aglycon moiety.
24342348	5	37	theme	ribopyranosyl	956:968	arg1	alkanamides					970:980	Several ribopyranosyl alkanamides	948:980	Several ribopyranosyl alkanamides	948:980	Several ribopyranosyl alkanamides have been synthesized and crystal structures of three of them have been solved.
24342348	0	38	theme	C5-hydroxymethyl	32:47	arg1	group					49:53	C5-hydroxymethyl group	32:53	C5-hydroxymethyl group	32:53	Examination of the influence of C5-hydroxymethyl group and configurations of hydroxyl groups at C2, C3, and C4 stereocentres on the N-glycosidic torsion: synthesis and X-ray crystallographic investigation of N-(D-ribopyranosyl)alkanamides as N-glycoprotein linkage region analogs.
24342348	4	39	theme	molecular	928:936	arg1	assembly					938:945	molecular assembly	928:945	molecular assembly	928:945	The present work was undertaken to assess the influence of ribose on the N-glycosidic torsions and molecular assembly.
24342348	5	40	theme	three	1030:1034	arg1	structures					1016:1025	crystal structures	1008:1025	crystal structures of three of them	1008:1042	Several ribopyranosyl alkanamides have been synthesized and crystal structures of three of them have been solved.
24342348	7	41	theme	group	1360:1364	arg1	importance					1329:1338	the importance	1325:1338	the importance of C5-hydroxymethyl group and hydroxyl group configurations	1325:1398	This study revealed the importance of C5-hydroxymethyl group and hydroxyl group configurations at C2, C3, and C4 stereocentres in controlling the N-glycosidic torsions.
24342348	7	42	theme	C4	1415:1416	arg1	stereocentres					1418:1430	C2, C3, and C4 stereocentres	1403:1430	C2, C3, and C4 stereocentres	1403:1430	This study revealed the importance of C5-hydroxymethyl group and hydroxyl group configurations at C2, C3, and C4 stereocentres in controlling the N-glycosidic torsions.
24342348	7	43	dep	group	1360:1364	arg1	configurations					1385:1398	configurations	1385:1398	configurations	1385:1398	This study revealed the importance of C5-hydroxymethyl group and hydroxyl group configurations at C2, C3, and C4 stereocentres in controlling the N-glycosidic torsions.
24342348	7	44	theme	C3	1407:1408	arg1	stereocentres					1418:1430	C2, C3, and C4 stereocentres	1403:1430	C2, C3, and C4 stereocentres	1403:1430	This study revealed the importance of C5-hydroxymethyl group and hydroxyl group configurations at C2, C3, and C4 stereocentres in controlling the N-glycosidic torsions.
24342348	4	45	theme	ribose	888:893	arg1	influence					875:883	the influence	871:883	the influence of ribose on the N-glycosidic torsions and molecular assembly	871:945	The present work was undertaken to assess the influence of ribose on the N-glycosidic torsions and molecular assembly.
24342348	5	46	theme	them	1039:1042	arg1	them					1039:1042	them	1039:1042	them	1039:1042	Several ribopyranosyl alkanamides have been synthesized and crystal structures of three of them have been solved.
24342348	5	46	theme	them	1039:1042	arg1	three					1030:1034	three	1030:1034	three	1030:1034	Several ribopyranosyl alkanamides have been synthesized and crystal structures of three of them have been solved.
24342348	0	47	theme	group	49:53	arg1	influence					19:27	the influence	15:27	the influence of C5-hydroxymethyl group and configurations of hydroxyl groups	15:91	Examination of the influence of C5-hydroxymethyl group and configurations of hydroxyl groups at C2, C3, and C4 stereocentres on the N-glycosidic torsion: synthesis and X-ray crystallographic investigation of N-(D-ribopyranosyl)alkanamides as N-glycoprotein linkage region analogs.
24342348	6	48	theme	deviations	1199:1208	arg1	range					1190:1194	the wide range	1181:1194	the wide range of deviations in their ϕN values	1181:1227	A comprehensive crystal structure analysis of ribosyl alkanamides along with xylosyl and arabinosyl alkanamides showed the wide range of deviations in their ϕN values as compared to the negligible deviation shown by hexopyranosyl alkanamides.
24342348	0	49	from	C3	100:101	arg1	Examination					0:10	Examination	0:10	Examination of the influence of C5-hydroxymethyl group and configurations of hydroxyl groups at C2, C3, and C4	0:109	Examination of the influence of C5-hydroxymethyl group and configurations of hydroxyl groups at C2, C3, and C4 stereocentres on the N-glycosidic torsion: synthesis and X-ray crystallographic investigation of N-(D-ribopyranosyl)alkanamides as N-glycoprotein linkage region analogs.
24342348	4	50	theme	present	833:839	arg1	work					841:844	The present work	829:844	The present work	829:844	The present work was undertaken to assess the influence of ribose on the N-glycosidic torsions and molecular assembly.
24342348	1	51	gly	glycosylation	290:302	arg2	bacteria					369:376	bacteria	369:376	bacteria	369:376	N-Linked glycosylation is not only present in eukaryotes but also occurs in archaea and bacteria and is mainly characterized by the β-glucosylamine linkage to the asparagine (GlcNAcβAsn).
24342348	1	51	gly	glycosylation	290:302	arg2	archaea					357:363	archaea	357:363	archaea	357:363	N-Linked glycosylation is not only present in eukaryotes but also occurs in archaea and bacteria and is mainly characterized by the β-glucosylamine linkage to the asparagine (GlcNAcβAsn).
24342348	1	51	gly	glycosylation	290:302	arg2	eukaryotes					327:336	eukaryotes	327:336	eukaryotes	327:336	N-Linked glycosylation is not only present in eukaryotes but also occurs in archaea and bacteria and is mainly characterized by the β-glucosylamine linkage to the asparagine (GlcNAcβAsn).
24342348	2	52	from	variation	657:665	arg1	part					681:684	the glycan part	670:684	the glycan part	670:684	Earlier crystallographic studies aimed at understanding the structural significance of the linkage region constituents revealed that N-glycosidic torsion, ϕN is influenced considerably by variation in the glycan part as compared to the aglycon moiety.
24342348	2	53	theme	constituents	575:586	arg1	significance					540:551	the structural significance	525:551	the structural significance of the linkage region constituents	525:586	Earlier crystallographic studies aimed at understanding the structural significance of the linkage region constituents revealed that N-glycosidic torsion, ϕN is influenced considerably by variation in the glycan part as compared to the aglycon moiety.
24342348	6	54	dep	xylosyl	1139:1145	arg1	alkanamides					1162:1172	alkanamides	1162:1172	alkanamides	1162:1172	A comprehensive crystal structure analysis of ribosyl alkanamides along with xylosyl and arabinosyl alkanamides showed the wide range of deviations in their ϕN values as compared to the negligible deviation shown by hexopyranosyl alkanamides.
24342348	1	55	attach	present	316:322	arg2	glycosylation					290:302	N-Linked glycosylation	281:302	N-Linked glycosylation	281:302	N-Linked glycosylation is not only present in eukaryotes but also occurs in archaea and bacteria and is mainly characterized by the β-glucosylamine linkage to the asparagine (GlcNAcβAsn).
24342348	1	55	attach	present	316:322	arg1	eukaryotes					327:336	eukaryotes	327:336	eukaryotes	327:336	N-Linked glycosylation is not only present in eukaryotes but also occurs in archaea and bacteria and is mainly characterized by the β-glucosylamine linkage to the asparagine (GlcNAcβAsn).
24342348	3	56	theme	model	800:804	arg1	GlcNAcβNHAc					816:826	GlcNAcβNHAc	816:826	GlcNAcβNHAc	816:826	The ϕN value observed for XylβNHAc deviated maximum as compared to that of the model compound, GlcNAcβNHAc.
24342348	3	56	theme	model	800:804	arg1	compound					806:813	the model compound	796:813	the model compound	796:813	The ϕN value observed for XylβNHAc deviated maximum as compared to that of the model compound, GlcNAcβNHAc.
24342348	0	57	theme	N-glycosidic	132:143	arg1	torsion					145:151	the N-glycosidic torsion	128:151	the N-glycosidic torsion	128:151	Examination of the influence of C5-hydroxymethyl group and configurations of hydroxyl groups at C2, C3, and C4 stereocentres on the N-glycosidic torsion: synthesis and X-ray crystallographic investigation of N-(D-ribopyranosyl)alkanamides as N-glycoprotein linkage region analogs.
24342348	0	58	from	C2	96:97	arg1	Examination					0:10	Examination	0:10	Examination of the influence of C5-hydroxymethyl group and configurations of hydroxyl groups at C2, C3, and C4	0:109	Examination of the influence of C5-hydroxymethyl group and configurations of hydroxyl groups at C2, C3, and C4 stereocentres on the N-glycosidic torsion: synthesis and X-ray crystallographic investigation of N-(D-ribopyranosyl)alkanamides as N-glycoprotein linkage region analogs.
24342348	5	59	theme	crystal	1008:1014	arg1	structures					1016:1025	crystal structures	1008:1025	crystal structures of three of them	1008:1042	Several ribopyranosyl alkanamides have been synthesized and crystal structures of three of them have been solved.
24342348	7	60	theme	C5-hydroxymethyl	1343:1358	arg1	group					1360:1364	C5-hydroxymethyl group	1343:1364	C5-hydroxymethyl group	1343:1364	This study revealed the importance of C5-hydroxymethyl group and hydroxyl group configurations at C2, C3, and C4 stereocentres in controlling the N-glycosidic torsions.
24342348	0	61	from	C4	108:109	arg1	Examination					0:10	Examination	0:10	Examination of the influence of C5-hydroxymethyl group and configurations of hydroxyl groups at C2, C3, and C4	0:109	Examination of the influence of C5-hydroxymethyl group and configurations of hydroxyl groups at C2, C3, and C4 stereocentres on the N-glycosidic torsion: synthesis and X-ray crystallographic investigation of N-(D-ribopyranosyl)alkanamides as N-glycoprotein linkage region analogs.
24342348	1	62	theme	β-glucosylamine	413:427	arg1	linkage					429:435	the β-glucosylamine linkage	409:435	the β-glucosylamine linkage to the asparagine (GlcNAcβAsn)	409:466	N-Linked glycosylation is not only present in eukaryotes but also occurs in archaea and bacteria and is mainly characterized by the β-glucosylamine linkage to the asparagine (GlcNAcβAsn).
24342348	6	63	theme	ϕN	1219:1220	arg1	values					1222:1227	their ϕN values	1213:1227	their ϕN values	1213:1227	A comprehensive crystal structure analysis of ribosyl alkanamides along with xylosyl and arabinosyl alkanamides showed the wide range of deviations in their ϕN values as compared to the negligible deviation shown by hexopyranosyl alkanamides.
24342348	7	64	theme	hydroxyl	1370:1377	arg1	group					1379:1383	hydroxyl group	1370:1383	hydroxyl group	1370:1383	This study revealed the importance of C5-hydroxymethyl group and hydroxyl group configurations at C2, C3, and C4 stereocentres in controlling the N-glycosidic torsions.
24342348	0	65	theme	configurations	59:72	arg1	influence					19:27	the influence	15:27	the influence of C5-hydroxymethyl group and configurations of hydroxyl groups	15:91	Examination of the influence of C5-hydroxymethyl group and configurations of hydroxyl groups at C2, C3, and C4 stereocentres on the N-glycosidic torsion: synthesis and X-ray crystallographic investigation of N-(D-ribopyranosyl)alkanamides as N-glycoprotein linkage region analogs.
24342348	4	66	from	influence	875:883	arg1	torsions					915:922	the N-glycosidic torsions	898:922	the N-glycosidic torsions	898:922	The present work was undertaken to assess the influence of ribose on the N-glycosidic torsions and molecular assembly.
24342348	4	66	from	influence	875:883	arg1	assembly					938:945	molecular assembly	928:945	molecular assembly	928:945	The present work was undertaken to assess the influence of ribose on the N-glycosidic torsions and molecular assembly.
24342348	6	67	theme	alkanamides	1116:1126	arg1	analysis					1096:1103	A comprehensive crystal structure analysis	1062:1103	A comprehensive crystal structure analysis of ribosyl alkanamides along with xylosyl and arabinosyl alkanamides	1062:1172	A comprehensive crystal structure analysis of ribosyl alkanamides along with xylosyl and arabinosyl alkanamides showed the wide range of deviations in their ϕN values as compared to the negligible deviation shown by hexopyranosyl alkanamides.
25104395	1	0	theme	non-reducing	188:199	arg1	4'-OH					201:205	the non-reducing 4'-OH	184:205	the non-reducing 4'-OH	184:205	The synthesis of three fluorogenic chitobiosyl derivatives, modified at the non-reducing 4'-OH with, either a methyl, an isopropyl or a cyclohexylmethyl substituent, is described.
25104395	2	1	theme	Michaelis-Menten	395:410	arg1	kinetics					412:419	Michaelis-Menten kinetics	395:419	Michaelis-Menten kinetics	395:419	The 4'-capped 4-methylumbelliferyl chitobiosides are hydrolysed by the human chitinase CHIT1 following Michaelis-Menten kinetics and in contrast to unmodified chitobiosyl-4-methylumbelliferone do not undergo transglycosylation.
25104395	2	2	theme	4-methylumbelliferyl	306:325	arg1	chitobiosides					327:339	The 4'-capped 4-methylumbelliferyl chitobiosides	292:339	The 4'-capped 4-methylumbelliferyl chitobiosides	292:339	The 4'-capped 4-methylumbelliferyl chitobiosides are hydrolysed by the human chitinase CHIT1 following Michaelis-Menten kinetics and in contrast to unmodified chitobiosyl-4-methylumbelliferone do not undergo transglycosylation.
25104395	2	3	dep	hydrolysed	345:354	arg1	undergo					492:498	undergo	492:498	do not undergo transglycosylation	485:517	The 4'-capped 4-methylumbelliferyl chitobiosides are hydrolysed by the human chitinase CHIT1 following Michaelis-Menten kinetics and in contrast to unmodified chitobiosyl-4-methylumbelliferone do not undergo transglycosylation.
25104395	2	4	theme	4'-capped	296:304	arg1	chitobiosides					327:339	The 4'-capped 4-methylumbelliferyl chitobiosides	292:339	The 4'-capped 4-methylumbelliferyl chitobiosides	292:339	The 4'-capped 4-methylumbelliferyl chitobiosides are hydrolysed by the human chitinase CHIT1 following Michaelis-Menten kinetics and in contrast to unmodified chitobiosyl-4-methylumbelliferone do not undergo transglycosylation.
25104395	1	5	dep	derivatives	159:169	arg1	either					213:218	either	213:218	either	213:218	The synthesis of three fluorogenic chitobiosyl derivatives, modified at the non-reducing 4'-OH with, either a methyl, an isopropyl or a cyclohexylmethyl substituent, is described.
25104395	2	6	theme	unmodified	440:449	arg1	chitobiosyl-4-methylumbelliferone					451:483	unmodified chitobiosyl-4-methylumbelliferone	440:483	unmodified chitobiosyl-4-methylumbelliferone	440:483	The 4'-capped 4-methylumbelliferyl chitobiosides are hydrolysed by the human chitinase CHIT1 following Michaelis-Menten kinetics and in contrast to unmodified chitobiosyl-4-methylumbelliferone do not undergo transglycosylation.
25104395	3	7	theme	fluorogenic	697:707	arg1	substrate					709:717	fluorogenic substrate	697:717	fluorogenic substrate to monitor CHIT1 activity as a marker for Gaucher disease state	697:781	The compounds are also relatively poor hexosaminidase substrates and thus provide useful alternatives to 4'-deoxychitobiosyl-4-methylumbelliferone, previously reported by us as fluorogenic substrate to monitor CHIT1 activity as a marker for Gaucher disease state.
25104395	3	8	theme	poor	554:557	arg1	compounds					524:532	The compounds	520:532	The compounds	520:532	The compounds are also relatively poor hexosaminidase substrates and thus provide useful alternatives to 4'-deoxychitobiosyl-4-methylumbelliferone, previously reported by us as fluorogenic substrate to monitor CHIT1 activity as a marker for Gaucher disease state.
25104395	3	8	theme	poor	554:557	arg1	substrates					574:583	relatively poor hexosaminidase substrates	543:583	relatively poor hexosaminidase substrates	543:583	The compounds are also relatively poor hexosaminidase substrates and thus provide useful alternatives to 4'-deoxychitobiosyl-4-methylumbelliferone, previously reported by us as fluorogenic substrate to monitor CHIT1 activity as a marker for Gaucher disease state.
25104395	1	9	theme	fluorogenic	135:145	arg1	derivatives					159:169	three fluorogenic chitobiosyl derivatives	129:169	three fluorogenic chitobiosyl derivatives	129:169	The synthesis of three fluorogenic chitobiosyl derivatives, modified at the non-reducing 4'-OH with, either a methyl, an isopropyl or a cyclohexylmethyl substituent, is described.
25104395	3	10	theme	hexosaminidase	559:572	arg1	compounds					524:532	The compounds	520:532	The compounds	520:532	The compounds are also relatively poor hexosaminidase substrates and thus provide useful alternatives to 4'-deoxychitobiosyl-4-methylumbelliferone, previously reported by us as fluorogenic substrate to monitor CHIT1 activity as a marker for Gaucher disease state.
25104395	3	10	theme	hexosaminidase	559:572	arg1	substrates					574:583	relatively poor hexosaminidase substrates	543:583	relatively poor hexosaminidase substrates	543:583	The compounds are also relatively poor hexosaminidase substrates and thus provide useful alternatives to 4'-deoxychitobiosyl-4-methylumbelliferone, previously reported by us as fluorogenic substrate to monitor CHIT1 activity as a marker for Gaucher disease state.
25104395	3	11	theme	useful	602:607	arg1	alternatives					609:620	useful alternatives	602:620	useful alternatives	602:620	The compounds are also relatively poor hexosaminidase substrates and thus provide useful alternatives to 4'-deoxychitobiosyl-4-methylumbelliferone, previously reported by us as fluorogenic substrate to monitor CHIT1 activity as a marker for Gaucher disease state.
25104395	3	12	theme	CHIT1	730:734	arg1	activity					736:743	CHIT1 activity	730:743	CHIT1 activity	730:743	The compounds are also relatively poor hexosaminidase substrates and thus provide useful alternatives to 4'-deoxychitobiosyl-4-methylumbelliferone, previously reported by us as fluorogenic substrate to monitor CHIT1 activity as a marker for Gaucher disease state.
25104395	3	12	theme	CHIT1	730:734	arg1	marker					750:755	a marker	748:755	a marker for Gaucher disease state	748:781	The compounds are also relatively poor hexosaminidase substrates and thus provide useful alternatives to 4'-deoxychitobiosyl-4-methylumbelliferone, previously reported by us as fluorogenic substrate to monitor CHIT1 activity as a marker for Gaucher disease state.
25104395	0	13	theme	4'-O-alkyl-chitobiosyl-4-methylumbelliferone	24:67	arg1	synthesis					11:19	synthesis	11:19	synthesis	11:19	Design and synthesis of 4'-O-alkyl-chitobiosyl-4-methylumbelliferone as human chitinase fluorogenic substrates.
25104395	0	13	theme	4'-O-alkyl-chitobiosyl-4-methylumbelliferone	24:67	arg1	Design					0:5	Design	0:5	Design	0:5	Design and synthesis of 4'-O-alkyl-chitobiosyl-4-methylumbelliferone as human chitinase fluorogenic substrates.
25104395	3	14	theme	Gaucher	761:767	arg1	disease					769:775	Gaucher disease	761:775	Gaucher disease state	761:781	The compounds are also relatively poor hexosaminidase substrates and thus provide useful alternatives to 4'-deoxychitobiosyl-4-methylumbelliferone, previously reported by us as fluorogenic substrate to monitor CHIT1 activity as a marker for Gaucher disease state.
25104395	0	15	theme	chitinase	78:86	arg1	substrates					100:109	human chitinase fluorogenic substrates	72:109	human chitinase fluorogenic substrates	72:109	Design and synthesis of 4'-O-alkyl-chitobiosyl-4-methylumbelliferone as human chitinase fluorogenic substrates.
25104395	3	16	theme	disease	769:775	arg1	state					777:781	Gaucher disease state	761:781	Gaucher disease state	761:781	The compounds are also relatively poor hexosaminidase substrates and thus provide useful alternatives to 4'-deoxychitobiosyl-4-methylumbelliferone, previously reported by us as fluorogenic substrate to monitor CHIT1 activity as a marker for Gaucher disease state.
25104395	1	17	theme	chitobiosyl	147:157	arg1	derivatives					159:169	three fluorogenic chitobiosyl derivatives	129:169	three fluorogenic chitobiosyl derivatives	129:169	The synthesis of three fluorogenic chitobiosyl derivatives, modified at the non-reducing 4'-OH with, either a methyl, an isopropyl or a cyclohexylmethyl substituent, is described.
25104395	0	18	theme	human	72:76	arg1	substrates					100:109	human chitinase fluorogenic substrates	72:109	human chitinase fluorogenic substrates	72:109	Design and synthesis of 4'-O-alkyl-chitobiosyl-4-methylumbelliferone as human chitinase fluorogenic substrates.
25104395	1	19	theme	derivatives	159:169	arg1	synthesis					116:124	The synthesis	112:124	The synthesis of three fluorogenic chitobiosyl derivatives, modified at the non-reducing 4'-OH with, either a methyl, an isopropyl or a cyclohexylmethyl substituent,	112:276	The synthesis of three fluorogenic chitobiosyl derivatives, modified at the non-reducing 4'-OH with, either a methyl, an isopropyl or a cyclohexylmethyl substituent, is described.
25104395	2	20	theme	chitinase	369:377	arg1	CHIT1					379:383	the human chitinase CHIT1	359:383	the human chitinase CHIT1 following Michaelis-Menten kinetics	359:419	The 4'-capped 4-methylumbelliferyl chitobiosides are hydrolysed by the human chitinase CHIT1 following Michaelis-Menten kinetics and in contrast to unmodified chitobiosyl-4-methylumbelliferone do not undergo transglycosylation.
25104395	2	21	theme	human	363:367	arg1	CHIT1					379:383	the human chitinase CHIT1	359:383	the human chitinase CHIT1 following Michaelis-Menten kinetics	359:419	The 4'-capped 4-methylumbelliferyl chitobiosides are hydrolysed by the human chitinase CHIT1 following Michaelis-Menten kinetics and in contrast to unmodified chitobiosyl-4-methylumbelliferone do not undergo transglycosylation.
25104395	2	22	dep	chitobiosyl-4-methylumbelliferone	451:483	arg1	contrast					428:435	contrast	428:435	contrast	428:435	The 4'-capped 4-methylumbelliferyl chitobiosides are hydrolysed by the human chitinase CHIT1 following Michaelis-Menten kinetics and in contrast to unmodified chitobiosyl-4-methylumbelliferone do not undergo transglycosylation.
25104395	1	23	theme	cyclohexylmethyl	248:263	arg1	substituent					265:275	a cyclohexylmethyl substituent	246:275	a cyclohexylmethyl substituent	246:275	The synthesis of three fluorogenic chitobiosyl derivatives, modified at the non-reducing 4'-OH with, either a methyl, an isopropyl or a cyclohexylmethyl substituent, is described.
25104395	1	23	theme	cyclohexylmethyl	248:263	arg1	methyl					222:227	a methyl	220:227	a methyl	220:227	The synthesis of three fluorogenic chitobiosyl derivatives, modified at the non-reducing 4'-OH with, either a methyl, an isopropyl or a cyclohexylmethyl substituent, is described.
25104395	0	24	theme	fluorogenic	88:98	arg1	substrates					100:109	human chitinase fluorogenic substrates	72:109	human chitinase fluorogenic substrates	72:109	Design and synthesis of 4'-O-alkyl-chitobiosyl-4-methylumbelliferone as human chitinase fluorogenic substrates.
29050620	5	0	theme	in	791:792	arg1	invasion					815:822	in vitro melanoma cells invasion	791:822	in vitro melanoma cells invasion	791:822	MG-Pe was non-cytotoxic, but reduced in vitro melanoma cells invasion.
29050620	8	1	theme	promising	1123:1131	arg1	molecule					1147:1154	a promising anti-melanoma molecule	1121:1154	a promising anti-melanoma molecule capable of switching melanoma cells to a non-invasive phenotype with no toxicity to melanoma-bearing mice	1121:1260	Mg-Pe was shown to be a promising anti-melanoma molecule capable of switching melanoma cells to a non-invasive phenotype with no toxicity to melanoma-bearing mice.
29050620	8	1	theme	promising	1123:1131	arg1	Mg-Pe					1099:1103	Mg-Pe	1099:1103	Mg-Pe	1099:1103	Mg-Pe was shown to be a promising anti-melanoma molecule capable of switching melanoma cells to a non-invasive phenotype with no toxicity to melanoma-bearing mice.
29050620	3	2	theme	α-d-galactopyranosyl	506:525	arg1	residues					563:570	(1→6)-linked α-d-galactopyranosyl and 3-O-methyl-α-d-galactopyranosyl residues	493:570	(1→6)-linked α-d-galactopyranosyl and 3-O-methyl-α-d-galactopyranosyl residues	493:570	A mannogalactan having a main chain of (1→6)-linked α-d-galactopyranosyl and 3-O-methyl-α-d-galactopyranosyl residues, both partially substituted at OH-2 by β-d-Manp (MG-Pe) single-unit was found.
29050620	6	3	theme	tumor	927:931	arg1	volume					933:938	the tumor volume	923:938	the tumor volume	923:938	Also, 50mg/kg MG-Pe administration to melanoma-bearing C57BL/6 mice up to 10days decreased in 60% the tumor volume compared to control.
29050620	6	4	theme	MG-Pe	839:843	arg1	administration					845:858	50mg/kg MG-Pe administration	831:858	50mg/kg MG-Pe administration to melanoma-bearing C57BL/6 mice up to 10days	831:904	Also, 50mg/kg MG-Pe administration to melanoma-bearing C57BL/6 mice up to 10days decreased in 60% the tumor volume compared to control.
29050620	2	5	theme	mono-	358:362	arg1	spectroscopy					386:397	NMR spectroscopy	382:397	NMR spectroscopy	382:397	Structural assignments were carried out using mono- and bidimensional NMR spectroscopy, monosaccharide composition, and methylation analyses.
29050620	0	6	theme	methylated	86:95	arg1	mannogalactan					97:109	the partially methylated mannogalactan	72:109	the partially methylated mannogalactan from Pleurotus eryngii	72:132	Safe therapeutics of murine melanoma model using a novel antineoplasic, the partially methylated mannogalactan from Pleurotus eryngii.
29050620	0	6	theme	methylated	86:95	arg1	antineoplasic					57:69	a novel antineoplasic	49:69	a novel antineoplasic	49:69	Safe therapeutics of murine melanoma model using a novel antineoplasic, the partially methylated mannogalactan from Pleurotus eryngii.
29050620	3	7	theme	3-O-methyl-α-d-galactopyranosyl	531:561	arg1	residues					563:570	(1→6)-linked α-d-galactopyranosyl and 3-O-methyl-α-d-galactopyranosyl residues	493:570	(1→6)-linked α-d-galactopyranosyl and 3-O-methyl-α-d-galactopyranosyl residues	493:570	A mannogalactan having a main chain of (1→6)-linked α-d-galactopyranosyl and 3-O-methyl-α-d-galactopyranosyl residues, both partially substituted at OH-2 by β-d-Manp (MG-Pe) single-unit was found.
29050620	8	8	theme	anti-melanoma	1133:1145	arg1	molecule					1147:1154	a promising anti-melanoma molecule	1121:1154	a promising anti-melanoma molecule capable of switching melanoma cells to a non-invasive phenotype with no toxicity to melanoma-bearing mice	1121:1260	Mg-Pe was shown to be a promising anti-melanoma molecule capable of switching melanoma cells to a non-invasive phenotype with no toxicity to melanoma-bearing mice.
29050620	8	8	theme	anti-melanoma	1133:1145	arg1	Mg-Pe					1099:1103	Mg-Pe	1099:1103	Mg-Pe	1099:1103	Mg-Pe was shown to be a promising anti-melanoma molecule capable of switching melanoma cells to a non-invasive phenotype with no toxicity to melanoma-bearing mice.
29050620	1	9	theme	mushroom	210:217	arg1	basidiocarps					253:264	edible mushroom Pleurotus eryngii ("King Oyster") basidiocarps	203:264	edible mushroom Pleurotus eryngii ("King Oyster") basidiocarps	203:264	A heteropolysaccharide was isolated by cold aqueous extraction from edible mushroom Pleurotus eryngii ("King Oyster") basidiocarps and its biological properties were evaluated.
29050620	1	9	theme	mushroom	210:217	arg1	"					250:250	"King Oyster"	238:250	"King Oyster"	238:250	A heteropolysaccharide was isolated by cold aqueous extraction from edible mushroom Pleurotus eryngii ("King Oyster") basidiocarps and its biological properties were evaluated.
29050620	1	10	theme	cold	174:177	arg1	extraction					187:196	cold aqueous extraction	174:196	cold aqueous extraction from edible mushroom Pleurotus eryngii ("King Oyster") basidiocarps	174:264	A heteropolysaccharide was isolated by cold aqueous extraction from edible mushroom Pleurotus eryngii ("King Oyster") basidiocarps and its biological properties were evaluated.
29050620	8	11	theme	capable	1156:1162	arg1	molecule					1147:1154	a promising anti-melanoma molecule	1121:1154	a promising anti-melanoma molecule capable of switching melanoma cells to a non-invasive phenotype with no toxicity to melanoma-bearing mice	1121:1260	Mg-Pe was shown to be a promising anti-melanoma molecule capable of switching melanoma cells to a non-invasive phenotype with no toxicity to melanoma-bearing mice.
29050620	8	11	theme	capable	1156:1162	arg1	Mg-Pe					1099:1103	Mg-Pe	1099:1103	Mg-Pe	1099:1103	Mg-Pe was shown to be a promising anti-melanoma molecule capable of switching melanoma cells to a non-invasive phenotype with no toxicity to melanoma-bearing mice.
29050620	5	12	theme	melanoma	800:807	arg1	invasion					815:822	in vitro melanoma cells invasion	791:822	in vitro melanoma cells invasion	791:822	MG-Pe was non-cytotoxic, but reduced in vitro melanoma cells invasion.
29050620	4	13	from	eryngii	695:701	arg1	effects					662:668	Biological effects	651:668	Biological effects of mannogalactan from P. eryngii (MG-Pe)	651:709	Biological effects of mannogalactan from P. eryngii (MG-Pe) were tested against murine melanoma cells.
29050620	2	14	theme	monosaccharide	400:413	arg1	composition					415:425	monosaccharide composition	400:425	monosaccharide composition	400:425	Structural assignments were carried out using mono- and bidimensional NMR spectroscopy, monosaccharide composition, and methylation analyses.
29050620	8	15	theme	melanoma	1177:1184	arg1	cells					1186:1190	melanoma cells	1177:1190	melanoma cells	1177:1190	Mg-Pe was shown to be a promising anti-melanoma molecule capable of switching melanoma cells to a non-invasive phenotype with no toxicity to melanoma-bearing mice.
29050620	7	16	theme	body	1072:1075	arg1	weight					1077:1082	body weight	1072:1082	body weight	1072:1082	Additionally, no changes were observed when biochemical profile, complete blood cells count (CBC), organs, and body weight were analyzed.
29050620	8	17	theme	melanoma-bearing	1240:1255	arg1	mice					1257:1260	melanoma-bearing mice	1240:1260	melanoma-bearing mice	1240:1260	Mg-Pe was shown to be a promising anti-melanoma molecule capable of switching melanoma cells to a non-invasive phenotype with no toxicity to melanoma-bearing mice.
29050620	0	18	theme	Safe	0:3	arg1	therapeutics					5:16	Safe therapeutics	0:16	Safe therapeutics of murine melanoma model using a novel antineoplasic, the partially methylated mannogalactan from Pleurotus eryngii.	0:133	Safe therapeutics of murine melanoma model using a novel antineoplasic, the partially methylated mannogalactan from Pleurotus eryngii.
29050620	2	19	theme	Structural	312:321	arg1	assignments					323:333	Structural assignments	312:333	Structural assignments	312:333	Structural assignments were carried out using mono- and bidimensional NMR spectroscopy, monosaccharide composition, and methylation analyses.
29050620	0	20	theme	murine	21:26	arg1	model					37:41	murine melanoma model	21:41	murine melanoma model	21:41	Safe therapeutics of murine melanoma model using a novel antineoplasic, the partially methylated mannogalactan from Pleurotus eryngii.
29050620	1	21	theme	biological	274:283	arg1	properties					285:294	its biological properties	270:294	its biological properties	270:294	A heteropolysaccharide was isolated by cold aqueous extraction from edible mushroom Pleurotus eryngii ("King Oyster") basidiocarps and its biological properties were evaluated.
29050620	1	22	from	basidiocarps	253:264	arg1	extraction					187:196	cold aqueous extraction	174:196	cold aqueous extraction from edible mushroom Pleurotus eryngii ("King Oyster") basidiocarps	174:264	A heteropolysaccharide was isolated by cold aqueous extraction from edible mushroom Pleurotus eryngii ("King Oyster") basidiocarps and its biological properties were evaluated.
29050620	3	23	dep	having	470:475	arg1	both					573:576	both	573:576	both	573:576	A mannogalactan having a main chain of (1→6)-linked α-d-galactopyranosyl and 3-O-methyl-α-d-galactopyranosyl residues, both partially substituted at OH-2 by β-d-Manp (MG-Pe) single-unit was found.
29050620	8	24	theme	non-invasive	1197:1208	arg1	phenotype					1210:1218	a non-invasive phenotype	1195:1218	a non-invasive phenotype with no toxicity to melanoma-bearing mice	1195:1260	Mg-Pe was shown to be a promising anti-melanoma molecule capable of switching melanoma cells to a non-invasive phenotype with no toxicity to melanoma-bearing mice.
29050620	1	25	theme	edible	203:208	arg1	mushroom					210:217	edible mushroom	203:217	edible mushroom Pleurotus eryngii ("King Oyster") basidiocarps	203:264	A heteropolysaccharide was isolated by cold aqueous extraction from edible mushroom Pleurotus eryngii ("King Oyster") basidiocarps and its biological properties were evaluated.
29050620	3	26	theme	β-d-Manp	611:618	arg1	single-unit					628:638	β-d-Manp (MG-Pe) single-unit	611:638	β-d-Manp (MG-Pe) single-unit	611:638	A mannogalactan having a main chain of (1→6)-linked α-d-galactopyranosyl and 3-O-methyl-α-d-galactopyranosyl residues, both partially substituted at OH-2 by β-d-Manp (MG-Pe) single-unit was found.
29050620	7	27	theme	complete	1026:1033	arg1	CBC					1054:1056	CBC	1054:1056	CBC	1054:1056	Additionally, no changes were observed when biochemical profile, complete blood cells count (CBC), organs, and body weight were analyzed.
29050620	7	27	theme	complete	1026:1033	arg1	count					1047:1051	complete blood cells count	1026:1051	complete blood cells count (CBC)	1026:1057	Additionally, no changes were observed when biochemical profile, complete blood cells count (CBC), organs, and body weight were analyzed.
29050620	8	28	with	phenotype	1210:1218	arg1	toxicity					1228:1235	no toxicity	1225:1235	no toxicity to melanoma-bearing mice	1225:1260	Mg-Pe was shown to be a promising anti-melanoma molecule capable of switching melanoma cells to a non-invasive phenotype with no toxicity to melanoma-bearing mice.
29050620	2	29	theme	methylation	432:442	arg1	analyses					444:451	methylation analyses	432:451	methylation analyses	432:451	Structural assignments were carried out using mono- and bidimensional NMR spectroscopy, monosaccharide composition, and methylation analyses.
29050620	0	30	theme	model	37:41	arg1	therapeutics					5:16	Safe therapeutics	0:16	Safe therapeutics of murine melanoma model using a novel antineoplasic, the partially methylated mannogalactan from Pleurotus eryngii.	0:133	Safe therapeutics of murine melanoma model using a novel antineoplasic, the partially methylated mannogalactan from Pleurotus eryngii.
29050620	4	31	theme	murine	731:736	arg1	cells					747:751	murine melanoma cells	731:751	murine melanoma cells	731:751	Biological effects of mannogalactan from P. eryngii (MG-Pe) were tested against murine melanoma cells.
29050620	0	32	theme	melanoma	28:35	arg1	model					37:41	murine melanoma model	21:41	murine melanoma model	21:41	Safe therapeutics of murine melanoma model using a novel antineoplasic, the partially methylated mannogalactan from Pleurotus eryngii.
29050620	5	33	theme	cells	809:813	arg1	invasion					815:822	in vitro melanoma cells invasion	791:822	in vitro melanoma cells invasion	791:822	MG-Pe was non-cytotoxic, but reduced in vitro melanoma cells invasion.
29050620	1	34	theme	King	239:242	arg1	basidiocarps					253:264	edible mushroom Pleurotus eryngii ("King Oyster") basidiocarps	203:264	edible mushroom Pleurotus eryngii ("King Oyster") basidiocarps	203:264	A heteropolysaccharide was isolated by cold aqueous extraction from edible mushroom Pleurotus eryngii ("King Oyster") basidiocarps and its biological properties were evaluated.
29050620	1	34	theme	King	239:242	arg1	"					250:250	"King Oyster"	238:250	"King Oyster"	238:250	A heteropolysaccharide was isolated by cold aqueous extraction from edible mushroom Pleurotus eryngii ("King Oyster") basidiocarps and its biological properties were evaluated.
29050620	7	35	theme	blood	1035:1039	arg1	CBC					1054:1056	CBC	1054:1056	CBC	1054:1056	Additionally, no changes were observed when biochemical profile, complete blood cells count (CBC), organs, and body weight were analyzed.
29050620	7	35	theme	blood	1035:1039	arg1	count					1047:1051	complete blood cells count	1026:1051	complete blood cells count (CBC)	1026:1057	Additionally, no changes were observed when biochemical profile, complete blood cells count (CBC), organs, and body weight were analyzed.
29050620	3	36	theme	main	479:482	arg1	chain					484:488	a main chain	477:488	a main chain of (1→6)-linked α-d-galactopyranosyl and 3-O-methyl-α-d-galactopyranosyl residues	477:570	A mannogalactan having a main chain of (1→6)-linked α-d-galactopyranosyl and 3-O-methyl-α-d-galactopyranosyl residues, both partially substituted at OH-2 by β-d-Manp (MG-Pe) single-unit was found.
29050620	3	37	dep	mannogalactan	456:468	arg1	having					470:475	having	470:475	having a main chain of (1→6)-linked α-d-galactopyranosyl and 3-O-methyl-α-d-galactopyranosyl residues	470:570	A mannogalactan having a main chain of (1→6)-linked α-d-galactopyranosyl and 3-O-methyl-α-d-galactopyranosyl residues, both partially substituted at OH-2 by β-d-Manp (MG-Pe) single-unit was found.
29050620	7	38	theme	cells	1041:1045	arg1	CBC					1054:1056	CBC	1054:1056	CBC	1054:1056	Additionally, no changes were observed when biochemical profile, complete blood cells count (CBC), organs, and body weight were analyzed.
29050620	7	38	theme	cells	1041:1045	arg1	count					1047:1051	complete blood cells count	1026:1051	complete blood cells count (CBC)	1026:1057	Additionally, no changes were observed when biochemical profile, complete blood cells count (CBC), organs, and body weight were analyzed.
29050620	4	39	theme	melanoma	738:745	arg1	cells					747:751	murine melanoma cells	731:751	murine melanoma cells	731:751	Biological effects of mannogalactan from P. eryngii (MG-Pe) were tested against murine melanoma cells.
29050620	6	40	dep	10days	899:904	arg1	up					893:894	up	893:894	up	893:894	Also, 50mg/kg MG-Pe administration to melanoma-bearing C57BL/6 mice up to 10days decreased in 60% the tumor volume compared to control.
29050620	5	41	dep	in	791:792	arg1	vitro					794:798	vitro	794:798	vitro	794:798	MG-Pe was non-cytotoxic, but reduced in vitro melanoma cells invasion.
29050620	6	42	theme	C57BL/6	880:886	arg1	mice					888:891	melanoma-bearing C57BL/6 mice	863:891	melanoma-bearing C57BL/6 mice	863:891	Also, 50mg/kg MG-Pe administration to melanoma-bearing C57BL/6 mice up to 10days decreased in 60% the tumor volume compared to control.
29050620	3	43	theme	MG-Pe	621:625	arg1	single-unit					628:638	β-d-Manp (MG-Pe) single-unit	611:638	β-d-Manp (MG-Pe) single-unit	611:638	A mannogalactan having a main chain of (1→6)-linked α-d-galactopyranosyl and 3-O-methyl-α-d-galactopyranosyl residues, both partially substituted at OH-2 by β-d-Manp (MG-Pe) single-unit was found.
29050620	3	44	theme	-linked	498:504	arg1	residues					563:570	(1→6)-linked α-d-galactopyranosyl and 3-O-methyl-α-d-galactopyranosyl residues	493:570	(1→6)-linked α-d-galactopyranosyl and 3-O-methyl-α-d-galactopyranosyl residues	493:570	A mannogalactan having a main chain of (1→6)-linked α-d-galactopyranosyl and 3-O-methyl-α-d-galactopyranosyl residues, both partially substituted at OH-2 by β-d-Manp (MG-Pe) single-unit was found.
29050620	6	45	theme	melanoma-bearing	863:878	arg1	mice					888:891	melanoma-bearing C57BL/6 mice	863:891	melanoma-bearing C57BL/6 mice	863:891	Also, 50mg/kg MG-Pe administration to melanoma-bearing C57BL/6 mice up to 10days decreased in 60% the tumor volume compared to control.
29050620	6	46	theme	50mg/kg	831:837	arg1	administration					845:858	50mg/kg MG-Pe administration	831:858	50mg/kg MG-Pe administration to melanoma-bearing C57BL/6 mice up to 10days	831:904	Also, 50mg/kg MG-Pe administration to melanoma-bearing C57BL/6 mice up to 10days decreased in 60% the tumor volume compared to control.
29050620	3	47	link	-linked	498:504	arg1	residues					563:570	(1→6)-linked α-d-galactopyranosyl and 3-O-methyl-α-d-galactopyranosyl residues	493:570	(1→6)-linked α-d-galactopyranosyl and 3-O-methyl-α-d-galactopyranosyl residues	493:570	A mannogalactan having a main chain of (1→6)-linked α-d-galactopyranosyl and 3-O-methyl-α-d-galactopyranosyl residues, both partially substituted at OH-2 by β-d-Manp (MG-Pe) single-unit was found.
29050620	7	48	theme	biochemical	1005:1015	arg1	profile					1017:1023	biochemical profile	1005:1023	biochemical profile	1005:1023	Additionally, no changes were observed when biochemical profile, complete blood cells count (CBC), organs, and body weight were analyzed.
29050620	3	49	theme	residues	563:570	arg1	chain					484:488	a main chain	477:488	a main chain of (1→6)-linked α-d-galactopyranosyl and 3-O-methyl-α-d-galactopyranosyl residues	477:570	A mannogalactan having a main chain of (1→6)-linked α-d-galactopyranosyl and 3-O-methyl-α-d-galactopyranosyl residues, both partially substituted at OH-2 by β-d-Manp (MG-Pe) single-unit was found.
29050620	6	50	dep	decreased	906:914	arg1	compared					940:947	compared	940:947	compared to control	940:958	Also, 50mg/kg MG-Pe administration to melanoma-bearing C57BL/6 mice up to 10days decreased in 60% the tumor volume compared to control.
29050620	4	51	theme	mannogalactan	673:685	arg1	effects					662:668	Biological effects	651:668	Biological effects of mannogalactan from P. eryngii (MG-Pe)	651:709	Biological effects of mannogalactan from P. eryngii (MG-Pe) were tested against murine melanoma cells.
29050620	4	52	theme	Biological	651:660	arg1	effects					662:668	Biological effects	651:668	Biological effects of mannogalactan from P. eryngii (MG-Pe)	651:709	Biological effects of mannogalactan from P. eryngii (MG-Pe) were tested against murine melanoma cells.
29050620	2	53	theme	NMR	382:384	arg1	spectroscopy					386:397	NMR spectroscopy	382:397	NMR spectroscopy	382:397	Structural assignments were carried out using mono- and bidimensional NMR spectroscopy, monosaccharide composition, and methylation analyses.
29050620	1	54	theme	aqueous	179:185	arg1	extraction					187:196	cold aqueous extraction	174:196	cold aqueous extraction from edible mushroom Pleurotus eryngii ("King Oyster") basidiocarps	174:264	A heteropolysaccharide was isolated by cold aqueous extraction from edible mushroom Pleurotus eryngii ("King Oyster") basidiocarps and its biological properties were evaluated.
29050620	0	55	from	eryngii	126:132	arg1	mannogalactan					97:109	the partially methylated mannogalactan	72:109	the partially methylated mannogalactan from Pleurotus eryngii	72:132	Safe therapeutics of murine melanoma model using a novel antineoplasic, the partially methylated mannogalactan from Pleurotus eryngii.
29050620	0	55	from	eryngii	126:132	arg1	antineoplasic					57:69	a novel antineoplasic	49:69	a novel antineoplasic	49:69	Safe therapeutics of murine melanoma model using a novel antineoplasic, the partially methylated mannogalactan from Pleurotus eryngii.
29050620	0	56	theme	novel	51:55	arg1	mannogalactan					97:109	the partially methylated mannogalactan	72:109	the partially methylated mannogalactan from Pleurotus eryngii	72:132	Safe therapeutics of murine melanoma model using a novel antineoplasic, the partially methylated mannogalactan from Pleurotus eryngii.
29050620	0	56	theme	novel	51:55	arg1	antineoplasic					57:69	a novel antineoplasic	49:69	a novel antineoplasic	49:69	Safe therapeutics of murine melanoma model using a novel antineoplasic, the partially methylated mannogalactan from Pleurotus eryngii.
29050620	2	57	theme	bidimensional	368:380	arg1	spectroscopy					386:397	NMR spectroscopy	382:397	NMR spectroscopy	382:397	Structural assignments were carried out using mono- and bidimensional NMR spectroscopy, monosaccharide composition, and methylation analyses.
29050620	1	58	theme	Oyster	244:249	arg1	basidiocarps					253:264	edible mushroom Pleurotus eryngii ("King Oyster") basidiocarps	203:264	edible mushroom Pleurotus eryngii ("King Oyster") basidiocarps	203:264	A heteropolysaccharide was isolated by cold aqueous extraction from edible mushroom Pleurotus eryngii ("King Oyster") basidiocarps and its biological properties were evaluated.
29050620	1	58	theme	Oyster	244:249	arg1	"					250:250	"King Oyster"	238:250	"King Oyster"	238:250	A heteropolysaccharide was isolated by cold aqueous extraction from edible mushroom Pleurotus eryngii ("King Oyster") basidiocarps and its biological properties were evaluated.
24831416	10	0	with	replicators	1741:1751	arg1	repertoires					1777:1787	narrower molecular repertoires	1758:1787	narrower molecular repertoires	1758:1787	This supports a prebiotic scenario initiated by fast-replicating assemblies with a high molecular diversity, evolving into more faithful replicators with narrower molecular repertoires.
24831416	3	1	theme	chemical	548:555	arg1	networks					569:576	chemical interaction networks	548:576	chemical interaction networks within molecular assemblies to emergent population dynamics	548:636	Here, we explore a prebiotic model that allows one to relate parameters of chemical interaction networks within molecular assemblies to emergent population dynamics.
24831416	9	2	theme	replication	1582:1592	arg1	fidelity					1594:1601	lower replication fidelity	1576:1601	lower replication fidelity	1576:1601	We found that compotypes with a larger intrinsic molecular repertoire show a higher intrinsic growth (r) and lower carrying capacity (K), as well as lower replication fidelity.
24831416	7	3	theme	global	1199:1204	arg1	parameters					1235:1244	the global chemical interaction network parameters	1195:1244	the global chemical interaction network parameters	1195:1244	In 1000 computer simulations, each embodying different parameter set of the global chemical interaction network parameters, we observed a wide range of behaviors.
24831416	3	4	theme	interaction	557:567	arg1	networks					569:576	chemical interaction networks	548:576	chemical interaction networks within molecular assemblies to emergent population dynamics	548:636	Here, we explore a prebiotic model that allows one to relate parameters of chemical interaction networks within molecular assemblies to emergent population dynamics.
24831416	2	5	from	evolution	332:340	arg1	populations					345:355	populations	345:355	populations of self-replicating molecules	345:385	Prebiotic models have often focused on evolution in populations of self-replicating molecules, without explicitly invoking the intermediate molecular-to-supramolecular transition.
24831416	1	6	theme	Present	72:78	arg1	life					80:83	Present life	72:83	Present life	72:83	Present life portrays a two-tier phenomenology: molecules compose supramolecular structures, such as cells or organisms, which in turn portray population behaviors, including selection, evolution and ecological dynamics.
24831416	10	7	theme	molecular	1767:1775	arg1	repertoires					1777:1787	narrower molecular repertoires	1758:1787	narrower molecular repertoires	1758:1787	This supports a prebiotic scenario initiated by fast-replicating assemblies with a high molecular diversity, evolving into more faithful replicators with narrower molecular repertoires.
24831416	1	8	theme	ecological	272:281	arg1	dynamics					283:290	ecological dynamics	272:290	ecological dynamics	272:290	Present life portrays a two-tier phenomenology: molecules compose supramolecular structures, such as cells or organisms, which in turn portray population behaviors, including selection, evolution and ecological dynamics.
24831416	8	9	dep	ecology	1376:1382	arg1	r-K					1385:1387	r-K	1385:1387	r-K	1385:1387	These were analyzed by a multi species logistic model often used for analyzing population ecology (r-K or Lotka-Volterra competition model).
24831416	8	9	dep	ecology	1376:1382	arg1	model					1419:1423	Lotka-Volterra competition model	1392:1423	Lotka-Volterra competition model	1392:1423	These were analyzed by a multi species logistic model often used for analyzing population ecology (r-K or Lotka-Volterra competition model).
24831416	3	10	theme	emergent	609:616	arg1	dynamics					629:636	emergent population dynamics	609:636	emergent population dynamics	609:636	Here, we explore a prebiotic model that allows one to relate parameters of chemical interaction networks within molecular assemblies to emergent population dynamics.
24831416	4	11	theme	graded	650:655	arg1	model					697:701	the graded autocatalysis replication domain (GARD) model	646:701	the graded autocatalysis replication domain (GARD) model	646:701	We use the graded autocatalysis replication domain (GARD) model, which simulates the network dynamics within amphiphile-containing molecular assemblies, and exhibits quasi-stationary compositional states termed compotype species.
24831416	4	12	theme	molecular	770:778	arg1	assemblies					780:789	amphiphile-containing molecular assemblies	748:789	amphiphile-containing molecular assemblies	748:789	We use the graded autocatalysis replication domain (GARD) model, which simulates the network dynamics within amphiphile-containing molecular assemblies, and exhibits quasi-stationary compositional states termed compotype species.
24831416	4	13	dep	compotype	850:858	arg1	species					860:866	species	860:866	compotype species	850:866	We use the graded autocatalysis replication domain (GARD) model, which simulates the network dynamics within amphiphile-containing molecular assemblies, and exhibits quasi-stationary compositional states termed compotype species.
24831416	7	14	theme	network	1227:1233	arg1	parameters					1235:1244	the global chemical interaction network parameters	1195:1244	the global chemical interaction network parameters	1195:1244	In 1000 computer simulations, each embodying different parameter set of the global chemical interaction network parameters, we observed a wide range of behaviors.
24831416	8	15	theme	competition	1407:1417	arg1	model					1419:1423	Lotka-Volterra competition model	1392:1423	Lotka-Volterra competition model	1392:1423	These were analyzed by a multi species logistic model often used for analyzing population ecology (r-K or Lotka-Volterra competition model).
24831416	2	16	theme	Prebiotic	293:301	arg1	models					303:308	Prebiotic models	293:308	Prebiotic models	293:308	Prebiotic models have often focused on evolution in populations of self-replicating molecules, without explicitly invoking the intermediate molecular-to-supramolecular transition.
24831416	10	17	theme	narrower	1758:1765	arg1	repertoires					1777:1787	narrower molecular repertoires	1758:1787	narrower molecular repertoires	1758:1787	This supports a prebiotic scenario initiated by fast-replicating assemblies with a high molecular diversity, evolving into more faithful replicators with narrower molecular repertoires.
24831416	2	18	theme	molecular-to-supramolecular	433:459	arg1	transition					461:470	the intermediate molecular-to-supramolecular transition	416:470	the intermediate molecular-to-supramolecular transition	416:470	Prebiotic models have often focused on evolution in populations of self-replicating molecules, without explicitly invoking the intermediate molecular-to-supramolecular transition.
24831416	6	19	theme	dynamics	1102:1109	arg1	parameters					1111:1120	population dynamics parameters	1091:1120	population dynamics parameters	1091:1120	The model allows us to ask how molecular network parameters influence assembly evolution and population dynamics parameters.
24831416	7	20	theme	parameters	1235:1244	arg1	parameters					1235:1244	the global chemical interaction network parameters	1195:1244	the global chemical interaction network parameters	1195:1244	In 1000 computer simulations, each embodying different parameter set of the global chemical interaction network parameters, we observed a wide range of behaviors.
24831416	7	20	theme	parameters	1235:1244	arg1	set					1188:1190	different parameter set	1168:1190	different parameter set of the global chemical interaction network parameters	1168:1244	In 1000 computer simulations, each embodying different parameter set of the global chemical interaction network parameters, we observed a wide range of behaviors.
24831416	10	21	with	assemblies	1669:1678	arg1	diversity					1702:1710	a high molecular diversity	1685:1710	a high molecular diversity	1685:1710	This supports a prebiotic scenario initiated by fast-replicating assemblies with a high molecular diversity, evolving into more faithful replicators with narrower molecular repertoires.
24831416	9	22	theme	intrinsic	1511:1519	arg1	growth					1521:1526	intrinsic growth	1511:1526	intrinsic growth (r)	1511:1530	We found that compotypes with a larger intrinsic molecular repertoire show a higher intrinsic growth (r) and lower carrying capacity (K), as well as lower replication fidelity.
24831416	9	22	theme	intrinsic	1511:1519	arg1	r					1529:1529	r	1529:1529	r	1529:1529	We found that compotypes with a larger intrinsic molecular repertoire show a higher intrinsic growth (r) and lower carrying capacity (K), as well as lower replication fidelity.
24831416	2	23	theme	intermediate	420:431	arg1	transition					461:470	the intermediate molecular-to-supramolecular transition	416:470	the intermediate molecular-to-supramolecular transition	416:470	Prebiotic models have often focused on evolution in populations of self-replicating molecules, without explicitly invoking the intermediate molecular-to-supramolecular transition.
24831416	0	24	theme	population	13:22	arg1	dynamics					24:31	Multispecies population dynamics	0:31	Multispecies population dynamics of prebiotic compositional assemblies	0:69	Multispecies population dynamics of prebiotic compositional assemblies.
24831416	9	25	theme	intrinsic	1466:1474	arg1	repertoire					1486:1495	a larger intrinsic molecular repertoire	1457:1495	a larger intrinsic molecular repertoire	1457:1495	We found that compotypes with a larger intrinsic molecular repertoire show a higher intrinsic growth (r) and lower carrying capacity (K), as well as lower replication fidelity.
24831416	7	26	theme	chemical	1206:1213	arg1	parameters					1235:1244	the global chemical interaction network parameters	1195:1244	the global chemical interaction network parameters	1195:1244	In 1000 computer simulations, each embodying different parameter set of the global chemical interaction network parameters, we observed a wide range of behaviors.
24831416	1	27	theme	two-tier	96:103	arg1	phenomenology					105:117	a two-tier phenomenology	94:117	a two-tier phenomenology	94:117	Present life portrays a two-tier phenomenology: molecules compose supramolecular structures, such as cells or organisms, which in turn portray population behaviors, including selection, evolution and ecological dynamics.
24831416	5	28	theme	replication-like	973:988	arg1	manner					990:995	a replication-like manner	971:995	a replication-like manner	971:995	These grow by catalyzed accretion, divide and propagate their compositional information to progeny in a replication-like manner.
24831416	0	29	theme	Multispecies	0:11	arg1	dynamics					24:31	Multispecies population dynamics	0:31	Multispecies population dynamics of prebiotic compositional assemblies	0:69	Multispecies population dynamics of prebiotic compositional assemblies.
24831416	6	30	theme	population	1091:1100	arg1	parameters					1111:1120	population dynamics parameters	1091:1120	population dynamics parameters	1091:1120	The model allows us to ask how molecular network parameters influence assembly evolution and population dynamics parameters.
24831416	7	31	theme	interaction	1215:1225	arg1	parameters					1235:1244	the global chemical interaction network parameters	1195:1244	the global chemical interaction network parameters	1195:1244	In 1000 computer simulations, each embodying different parameter set of the global chemical interaction network parameters, we observed a wide range of behaviors.
24831416	4	32	theme	compositional	822:834	arg1	states					836:841	quasi-stationary compositional states	805:841	quasi-stationary compositional states termed compotype species	805:866	We use the graded autocatalysis replication domain (GARD) model, which simulates the network dynamics within amphiphile-containing molecular assemblies, and exhibits quasi-stationary compositional states termed compotype species.
24831416	4	33	theme	network	724:730	arg1	dynamics					732:739	the network dynamics	720:739	the network dynamics within amphiphile-containing molecular assemblies	720:789	We use the graded autocatalysis replication domain (GARD) model, which simulates the network dynamics within amphiphile-containing molecular assemblies, and exhibits quasi-stationary compositional states termed compotype species.
24831416	8	34	theme	species	1317:1323	arg1	model					1334:1338	a multi species logistic model	1309:1338	a multi species logistic model often used for analyzing population ecology (r-K or Lotka-Volterra competition model)	1309:1424	These were analyzed by a multi species logistic model often used for analyzing population ecology (r-K or Lotka-Volterra competition model).
24831416	9	35	theme	carrying	1542:1549	arg1	K					1561:1561	K	1561:1561	K	1561:1561	We found that compotypes with a larger intrinsic molecular repertoire show a higher intrinsic growth (r) and lower carrying capacity (K), as well as lower replication fidelity.
24831416	9	35	theme	carrying	1542:1549	arg1	capacity					1551:1558	a higher intrinsic growth (r) and lower carrying capacity	1502:1558	a higher intrinsic growth (r) and lower carrying capacity (K)	1502:1562	We found that compotypes with a larger intrinsic molecular repertoire show a higher intrinsic growth (r) and lower carrying capacity (K), as well as lower replication fidelity.
24831416	3	36	theme	population	618:627	arg1	dynamics					629:636	emergent population dynamics	609:636	emergent population dynamics	609:636	Here, we explore a prebiotic model that allows one to relate parameters of chemical interaction networks within molecular assemblies to emergent population dynamics.
24831416	5	37	theme	compositional	931:943	arg1	information					945:955	their compositional information	925:955	their compositional information to progeny	925:966	These grow by catalyzed accretion, divide and propagate their compositional information to progeny in a replication-like manner.
24831416	8	38	theme	multi	1311:1315	arg1	species					1317:1323	multi species	1311:1323	a multi species logistic model often used for analyzing population ecology (r-K or Lotka-Volterra competition model)	1309:1424	These were analyzed by a multi species logistic model often used for analyzing population ecology (r-K or Lotka-Volterra competition model).
24831416	9	39	theme	larger	1459:1464	arg1	repertoire					1486:1495	a larger intrinsic molecular repertoire	1457:1495	a larger intrinsic molecular repertoire	1457:1495	We found that compotypes with a larger intrinsic molecular repertoire show a higher intrinsic growth (r) and lower carrying capacity (K), as well as lower replication fidelity.
24831416	3	40	theme	molecular	585:593	arg1	assemblies					595:604	molecular assemblies	585:604	molecular assemblies to emergent population dynamics	585:636	Here, we explore a prebiotic model that allows one to relate parameters of chemical interaction networks within molecular assemblies to emergent population dynamics.
24831416	2	41	theme	molecules	377:385	arg1	populations					345:355	populations	345:355	populations of self-replicating molecules	345:385	Prebiotic models have often focused on evolution in populations of self-replicating molecules, without explicitly invoking the intermediate molecular-to-supramolecular transition.
24831416	7	42	theme	behaviors	1275:1283	arg1	range					1266:1270	a wide range	1259:1270	a wide range of behaviors	1259:1283	In 1000 computer simulations, each embodying different parameter set of the global chemical interaction network parameters, we observed a wide range of behaviors.
24831416	4	43	theme	amphiphile-containing	748:768	arg1	assemblies					780:789	amphiphile-containing molecular assemblies	748:789	amphiphile-containing molecular assemblies	748:789	We use the graded autocatalysis replication domain (GARD) model, which simulates the network dynamics within amphiphile-containing molecular assemblies, and exhibits quasi-stationary compositional states termed compotype species.
24831416	0	44	theme	compositional	46:58	arg1	assemblies					60:69	prebiotic compositional assemblies	36:69	prebiotic compositional assemblies	36:69	Multispecies population dynamics of prebiotic compositional assemblies.
24831416	6	45	theme	assembly	1068:1075	arg1	evolution					1077:1085	assembly evolution	1068:1085	assembly evolution	1068:1085	The model allows us to ask how molecular network parameters influence assembly evolution and population dynamics parameters.
24831416	9	46	theme	lower	1576:1580	arg1	fidelity					1594:1601	lower replication fidelity	1576:1601	lower replication fidelity	1576:1601	We found that compotypes with a larger intrinsic molecular repertoire show a higher intrinsic growth (r) and lower carrying capacity (K), as well as lower replication fidelity.
24831416	8	47	theme	Lotka-Volterra	1392:1405	arg1	model					1419:1423	Lotka-Volterra competition model	1392:1423	Lotka-Volterra competition model	1392:1423	These were analyzed by a multi species logistic model often used for analyzing population ecology (r-K or Lotka-Volterra competition model).
24831416	8	48	theme	population	1365:1374	arg1	ecology					1376:1382	population ecology	1365:1382	population ecology (r-K or Lotka-Volterra competition model)	1365:1424	These were analyzed by a multi species logistic model often used for analyzing population ecology (r-K or Lotka-Volterra competition model).
24831416	1	49	theme	population	215:224	arg1	behaviors					226:234	population behaviors	215:234	population behaviors	215:234	Present life portrays a two-tier phenomenology: molecules compose supramolecular structures, such as cells or organisms, which in turn portray population behaviors, including selection, evolution and ecological dynamics.
24831416	0	50	theme	prebiotic	36:44	arg1	assemblies					60:69	prebiotic compositional assemblies	36:69	prebiotic compositional assemblies	36:69	Multispecies population dynamics of prebiotic compositional assemblies.
24831416	9	51	theme	molecular	1476:1484	arg1	repertoire					1486:1495	a larger intrinsic molecular repertoire	1457:1495	a larger intrinsic molecular repertoire	1457:1495	We found that compotypes with a larger intrinsic molecular repertoire show a higher intrinsic growth (r) and lower carrying capacity (K), as well as lower replication fidelity.
24831416	5	52	theme	catalyzed	883:891	arg1	accretion					893:901	catalyzed accretion	883:901	catalyzed accretion	883:901	These grow by catalyzed accretion, divide and propagate their compositional information to progeny in a replication-like manner.
24831416	9	53	dep	higher	1504:1509	arg1	growth					1521:1526	intrinsic growth	1511:1526	intrinsic growth (r)	1511:1530	We found that compotypes with a larger intrinsic molecular repertoire show a higher intrinsic growth (r) and lower carrying capacity (K), as well as lower replication fidelity.
24831416	9	53	dep	higher	1504:1509	arg1	r					1529:1529	r	1529:1529	r	1529:1529	We found that compotypes with a larger intrinsic molecular repertoire show a higher intrinsic growth (r) and lower carrying capacity (K), as well as lower replication fidelity.
24831416	10	54	theme	molecular	1692:1700	arg1	diversity					1702:1710	a high molecular diversity	1685:1710	a high molecular diversity	1685:1710	This supports a prebiotic scenario initiated by fast-replicating assemblies with a high molecular diversity, evolving into more faithful replicators with narrower molecular repertoires.
24831416	10	55	theme	prebiotic	1620:1628	arg1	scenario					1630:1637	a prebiotic scenario	1618:1637	a prebiotic scenario initiated by fast-replicating assemblies with a high molecular diversity, evolving into more faithful replicators with narrower molecular repertoires	1618:1787	This supports a prebiotic scenario initiated by fast-replicating assemblies with a high molecular diversity, evolving into more faithful replicators with narrower molecular repertoires.
24831416	7	56	theme	wide	1261:1264	arg1	range					1266:1270	a wide range	1259:1270	a wide range of behaviors	1259:1283	In 1000 computer simulations, each embodying different parameter set of the global chemical interaction network parameters, we observed a wide range of behaviors.
24831416	7	57	theme	different	1168:1176	arg1	parameters					1235:1244	the global chemical interaction network parameters	1195:1244	the global chemical interaction network parameters	1195:1244	In 1000 computer simulations, each embodying different parameter set of the global chemical interaction network parameters, we observed a wide range of behaviors.
24831416	7	57	theme	different	1168:1176	arg1	set					1188:1190	different parameter set	1168:1190	different parameter set of the global chemical interaction network parameters	1168:1244	In 1000 computer simulations, each embodying different parameter set of the global chemical interaction network parameters, we observed a wide range of behaviors.
24831416	9	58	dep	carrying	1542:1549	arg1	lower					1536:1540	lower	1536:1540	lower	1536:1540	We found that compotypes with a larger intrinsic molecular repertoire show a higher intrinsic growth (r) and lower carrying capacity (K), as well as lower replication fidelity.
24831416	2	59	theme	self-replicating	360:375	arg1	molecules					377:385	self-replicating molecules	360:385	self-replicating molecules	360:385	Prebiotic models have often focused on evolution in populations of self-replicating molecules, without explicitly invoking the intermediate molecular-to-supramolecular transition.
24831416	0	60	theme	assemblies	60:69	arg1	dynamics					24:31	Multispecies population dynamics	0:31	Multispecies population dynamics of prebiotic compositional assemblies	0:69	Multispecies population dynamics of prebiotic compositional assemblies.
24831416	6	61	theme	network	1039:1045	arg1	parameters					1047:1056	molecular network parameters	1029:1056	molecular network parameters	1029:1056	The model allows us to ask how molecular network parameters influence assembly evolution and population dynamics parameters.
24831416	7	62	theme	parameter	1178:1186	arg1	parameters					1235:1244	the global chemical interaction network parameters	1195:1244	the global chemical interaction network parameters	1195:1244	In 1000 computer simulations, each embodying different parameter set of the global chemical interaction network parameters, we observed a wide range of behaviors.
24831416	7	62	theme	parameter	1178:1186	arg1	set					1188:1190	different parameter set	1168:1190	different parameter set of the global chemical interaction network parameters	1168:1244	In 1000 computer simulations, each embodying different parameter set of the global chemical interaction network parameters, we observed a wide range of behaviors.
24831416	10	63	theme	fast-replicating	1652:1667	arg1	assemblies					1669:1678	fast-replicating assemblies	1652:1678	fast-replicating assemblies with a high molecular diversity, evolving into more faithful replicators with narrower molecular repertoires	1652:1787	This supports a prebiotic scenario initiated by fast-replicating assemblies with a high molecular diversity, evolving into more faithful replicators with narrower molecular repertoires.
24831416	8	64	theme	logistic	1325:1332	arg1	model					1334:1338	a multi species logistic model	1309:1338	a multi species logistic model often used for analyzing population ecology (r-K or Lotka-Volterra competition model)	1309:1424	These were analyzed by a multi species logistic model often used for analyzing population ecology (r-K or Lotka-Volterra competition model).
24831416	1	65	theme	supramolecular	138:151	arg1	structures					153:162	supramolecular structures	138:162	supramolecular structures	138:162	Present life portrays a two-tier phenomenology: molecules compose supramolecular structures, such as cells or organisms, which in turn portray population behaviors, including selection, evolution and ecological dynamics.
24831416	1	65	theme	supramolecular	138:151	arg1	organisms					182:190	organisms	182:190	organisms	182:190	Present life portrays a two-tier phenomenology: molecules compose supramolecular structures, such as cells or organisms, which in turn portray population behaviors, including selection, evolution and ecological dynamics.
24831416	1	65	theme	supramolecular	138:151	arg1	cells					173:177	cells	173:177	cells	173:177	Present life portrays a two-tier phenomenology: molecules compose supramolecular structures, such as cells or organisms, which in turn portray population behaviors, including selection, evolution and ecological dynamics.
24831416	6	66	theme	molecular	1029:1037	arg1	parameters					1047:1056	molecular network parameters	1029:1056	molecular network parameters	1029:1056	The model allows us to ask how molecular network parameters influence assembly evolution and population dynamics parameters.
24831416	10	67	theme	faithful	1732:1739	arg1	replicators					1741:1751	more faithful replicators	1727:1751	more faithful replicators with narrower molecular repertoires	1727:1787	This supports a prebiotic scenario initiated by fast-replicating assemblies with a high molecular diversity, evolving into more faithful replicators with narrower molecular repertoires.
24831416	7	68	theme	computer	1131:1138	arg1	each					1153:1156	each	1153:1156	each	1153:1156	In 1000 computer simulations, each embodying different parameter set of the global chemical interaction network parameters, we observed a wide range of behaviors.
24831416	7	68	theme	computer	1131:1138	arg1	simulations					1140:1150	1000 computer simulations	1126:1150	1000 computer simulations	1126:1150	In 1000 computer simulations, each embodying different parameter set of the global chemical interaction network parameters, we observed a wide range of behaviors.
24831416	4	69	theme	quasi-stationary	805:820	arg1	states					836:841	quasi-stationary compositional states	805:841	quasi-stationary compositional states termed compotype species	805:866	We use the graded autocatalysis replication domain (GARD) model, which simulates the network dynamics within amphiphile-containing molecular assemblies, and exhibits quasi-stationary compositional states termed compotype species.
24831416	9	70	with	compotypes	1441:1450	arg1	repertoire					1486:1495	a larger intrinsic molecular repertoire	1457:1495	a larger intrinsic molecular repertoire	1457:1495	We found that compotypes with a larger intrinsic molecular repertoire show a higher intrinsic growth (r) and lower carrying capacity (K), as well as lower replication fidelity.
24831416	4	71	theme	domain	683:688	arg1	model					697:701	the graded autocatalysis replication domain (GARD) model	646:701	the graded autocatalysis replication domain (GARD) model	646:701	We use the graded autocatalysis replication domain (GARD) model, which simulates the network dynamics within amphiphile-containing molecular assemblies, and exhibits quasi-stationary compositional states termed compotype species.
24831416	4	72	theme	replication	671:681	arg1	model					697:701	the graded autocatalysis replication domain (GARD) model	646:701	the graded autocatalysis replication domain (GARD) model	646:701	We use the graded autocatalysis replication domain (GARD) model, which simulates the network dynamics within amphiphile-containing molecular assemblies, and exhibits quasi-stationary compositional states termed compotype species.
24831416	3	73	theme	networks	569:576	arg1	parameters					534:543	parameters	534:543	parameters of chemical interaction networks within molecular assemblies to emergent population dynamics	534:636	Here, we explore a prebiotic model that allows one to relate parameters of chemical interaction networks within molecular assemblies to emergent population dynamics.
24831416	3	74	theme	prebiotic	492:500	arg1	model					502:506	a prebiotic model	490:506	a prebiotic model that allows one to relate parameters of chemical interaction networks within molecular assemblies to emergent population dynamics	490:636	Here, we explore a prebiotic model that allows one to relate parameters of chemical interaction networks within molecular assemblies to emergent population dynamics.
24831416	4	75	theme	GARD	691:694	arg1	model					697:701	the graded autocatalysis replication domain (GARD) model	646:701	the graded autocatalysis replication domain (GARD) model	646:701	We use the graded autocatalysis replication domain (GARD) model, which simulates the network dynamics within amphiphile-containing molecular assemblies, and exhibits quasi-stationary compositional states termed compotype species.
24831416	4	76	theme	autocatalysis	657:669	arg1	model					697:701	the graded autocatalysis replication domain (GARD) model	646:701	the graded autocatalysis replication domain (GARD) model	646:701	We use the graded autocatalysis replication domain (GARD) model, which simulates the network dynamics within amphiphile-containing molecular assemblies, and exhibits quasi-stationary compositional states termed compotype species.
24831416	10	77	theme	high	1687:1690	arg1	diversity					1702:1710	a high molecular diversity	1685:1710	a high molecular diversity	1685:1710	This supports a prebiotic scenario initiated by fast-replicating assemblies with a high molecular diversity, evolving into more faithful replicators with narrower molecular repertoires.
24831416	9	78	theme	higher	1504:1509	arg1	K					1561:1561	K	1561:1561	K	1561:1561	We found that compotypes with a larger intrinsic molecular repertoire show a higher intrinsic growth (r) and lower carrying capacity (K), as well as lower replication fidelity.
24831416	9	78	theme	higher	1504:1509	arg1	capacity					1551:1558	a higher intrinsic growth (r) and lower carrying capacity	1502:1558	a higher intrinsic growth (r) and lower carrying capacity (K)	1502:1562	We found that compotypes with a larger intrinsic molecular repertoire show a higher intrinsic growth (r) and lower carrying capacity (K), as well as lower replication fidelity.
27524274	8	0	theme	exclusion	962:970	arg1	chromatography					972:985	high pressure size exclusion chromatography	943:985	high pressure size exclusion chromatography (HPSEC)	943:993	On high pressure size exclusion chromatography (HPSEC), the elution pattern of the acid-extracted pectins showed that severe conditions were associated with lower hydrodynamic volume.
27524274	8	0	theme	exclusion	962:970	arg1	HPSEC					988:992	HPSEC	988:992	HPSEC	988:992	On high pressure size exclusion chromatography (HPSEC), the elution pattern of the acid-extracted pectins showed that severe conditions were associated with lower hydrodynamic volume.
27524274	1	1	theme	extraction	144:153	arg1	conditions					155:164	extraction conditions	144:164	extraction conditions	144:164	A central composite design was employed to determine the influence of extraction conditions on production yield and chemical composition of pectin from pomegranate peels.
27524274	8	2	theme	pectins	1038:1044	arg1	pattern					1008:1014	the elution pattern	996:1014	the elution pattern of the acid-extracted pectins	996:1044	On high pressure size exclusion chromatography (HPSEC), the elution pattern of the acid-extracted pectins showed that severe conditions were associated with lower hydrodynamic volume.
27524274	8	3	theme	elution	1000:1006	arg1	pattern					1008:1014	the elution pattern	996:1014	the elution pattern of the acid-extracted pectins	996:1044	On high pressure size exclusion chromatography (HPSEC), the elution pattern of the acid-extracted pectins showed that severe conditions were associated with lower hydrodynamic volume.
27524274	8	4	theme	size	957:960	arg1	chromatography					972:985	high pressure size exclusion chromatography	943:985	high pressure size exclusion chromatography (HPSEC)	943:993	On high pressure size exclusion chromatography (HPSEC), the elution pattern of the acid-extracted pectins showed that severe conditions were associated with lower hydrodynamic volume.
27524274	8	4	theme	size	957:960	arg1	HPSEC					988:992	HPSEC	988:992	HPSEC	988:992	On high pressure size exclusion chromatography (HPSEC), the elution pattern of the acid-extracted pectins showed that severe conditions were associated with lower hydrodynamic volume.
27524274	2	5	theme	processing	334:343	arg1	parameters					345:354	the three processing parameters	324:354	the three processing parameters (extraction duration, temperature and pH)	324:396	Response surface methodology (RSM) was used to quantify the integral effect of the three processing parameters (extraction duration, temperature and pH) on yield.
27524274	7	6	theme	extracted	901:909	arg1	pectins					911:917	the extracted pectins	897:917	the extracted pectins	897:917	Moreover, the degree of methylation varied with the extraction conditions and the extracted pectins were low methylated.
27524274	5	7	theme	extraction	625:634	arg1	value					612:616	Optimal temperature, duration and pH value	575:616	Optimal temperature, duration and pH value of the extraction	575:634	Optimal temperature, duration and pH value of the extraction were 86°C, 80min and 1.7, respectively.
27524274	2	8	from	effect	314:319	arg1	yield					401:405	yield	401:405	yield	401:405	Response surface methodology (RSM) was used to quantify the integral effect of the three processing parameters (extraction duration, temperature and pH) on yield.
27524274	2	9	dep	parameters	345:354	arg1	temperature					378:388	temperature	378:388	temperature	378:388	Response surface methodology (RSM) was used to quantify the integral effect of the three processing parameters (extraction duration, temperature and pH) on yield.
27524274	2	9	dep	parameters	345:354	arg1	duration					368:375	extraction duration	357:375	extraction duration	357:375	Response surface methodology (RSM) was used to quantify the integral effect of the three processing parameters (extraction duration, temperature and pH) on yield.
27524274	2	9	dep	parameters	345:354	arg1	pH					394:395	pH	394:395	pH	394:395	Response surface methodology (RSM) was used to quantify the integral effect of the three processing parameters (extraction duration, temperature and pH) on yield.
27524274	2	10	used	used	284:287	arg2	RSM					275:277	RSM	275:277	RSM	275:277	Response surface methodology (RSM) was used to quantify the integral effect of the three processing parameters (extraction duration, temperature and pH) on yield.
27524274	2	10	used	used	284:287	arg2	methodology					262:272	Response surface methodology	245:272	Response surface methodology (RSM)	245:278	Response surface methodology (RSM) was used to quantify the integral effect of the three processing parameters (extraction duration, temperature and pH) on yield.
27524274	1	11	theme	central	76:82	arg1	design					94:99	A central composite design	74:99	A central composite design	74:99	A central composite design was employed to determine the influence of extraction conditions on production yield and chemical composition of pectin from pomegranate peels.
27524274	1	12	from	influence	131:139	arg1	yield					180:184	production yield	169:184	production yield	169:184	A central composite design was employed to determine the influence of extraction conditions on production yield and chemical composition of pectin from pomegranate peels.
27524274	1	12	from	influence	131:139	arg1	composition					199:209	chemical composition	190:209	chemical composition	190:209	A central composite design was employed to determine the influence of extraction conditions on production yield and chemical composition of pectin from pomegranate peels.
27524274	2	13	theme	parameters	345:354	arg1	effect					314:319	the integral effect	301:319	the integral effect of the three processing parameters (extraction duration, temperature and pH) on yield	301:405	Response surface methodology (RSM) was used to quantify the integral effect of the three processing parameters (extraction duration, temperature and pH) on yield.
27524274	5	14	theme	temperature	583:593	arg1	value					612:616	Optimal temperature, duration and pH value	575:616	Optimal temperature, duration and pH value of the extraction	575:634	Optimal temperature, duration and pH value of the extraction were 86°C, 80min and 1.7, respectively.
27524274	5	15	theme	duration	596:603	arg1	value					612:616	Optimal temperature, duration and pH value	575:616	Optimal temperature, duration and pH value of the extraction	575:634	Optimal temperature, duration and pH value of the extraction were 86°C, 80min and 1.7, respectively.
27524274	6	16	theme	pectins	745:751	arg1	acid					687:690	The uronic acid	676:690	The uronic acid	676:690	The uronic acid and the total neutral sugar content of the extracted pectins ranged from 377 to 755mg/g and from 161 to 326mg/g, respectively.
27524274	6	16	theme	pectins	745:751	arg1	content					720:726	the total neutral sugar content	696:726	the total neutral sugar content of the extracted pectins	696:751	The uronic acid and the total neutral sugar content of the extracted pectins ranged from 377 to 755mg/g and from 161 to 326mg/g, respectively.
27524274	3	17	theme	second-order	410:421	arg1	model					434:438	A second-order polynomial model	408:438	A second-order polynomial model	408:438	A second-order polynomial model was developed for predicting the yield of pomegranate peels pectin based on the composite design.
27524274	8	18	theme	hydrodynamic	1103:1114	arg1	volume					1116:1121	lower hydrodynamic volume	1097:1121	lower hydrodynamic volume	1097:1121	On high pressure size exclusion chromatography (HPSEC), the elution pattern of the acid-extracted pectins showed that severe conditions were associated with lower hydrodynamic volume.
27524274	6	19	theme	extracted	735:743	arg1	pectins					745:751	the extracted pectins	731:751	the extracted pectins	731:751	The uronic acid and the total neutral sugar content of the extracted pectins ranged from 377 to 755mg/g and from 161 to 326mg/g, respectively.
27524274	1	20	theme	production	169:178	arg1	yield					180:184	production yield	169:184	production yield	169:184	A central composite design was employed to determine the influence of extraction conditions on production yield and chemical composition of pectin from pomegranate peels.
27524274	5	21	theme	pH	609:610	arg1	value					612:616	Optimal temperature, duration and pH value	575:616	Optimal temperature, duration and pH value of the extraction	575:634	Optimal temperature, duration and pH value of the extraction were 86°C, 80min and 1.7, respectively.
27524274	7	22	theme	extraction	871:880	arg1	conditions					882:891	the extraction conditions	867:891	the extraction conditions	867:891	Moreover, the degree of methylation varied with the extraction conditions and the extracted pectins were low methylated.
27524274	2	23	theme	integral	305:312	arg1	effect					314:319	the integral effect	301:319	the integral effect of the three processing parameters (extraction duration, temperature and pH) on yield	301:405	Response surface methodology (RSM) was used to quantify the integral effect of the three processing parameters (extraction duration, temperature and pH) on yield.
27524274	6	24	dep	755mg/g	772:778	arg1	to					769:770	to	769:770	to	769:770	The uronic acid and the total neutral sugar content of the extracted pectins ranged from 377 to 755mg/g and from 161 to 326mg/g, respectively.
27524274	8	25	theme	pressure	948:955	arg1	chromatography					972:985	high pressure size exclusion chromatography	943:985	high pressure size exclusion chromatography (HPSEC)	943:993	On high pressure size exclusion chromatography (HPSEC), the elution pattern of the acid-extracted pectins showed that severe conditions were associated with lower hydrodynamic volume.
27524274	8	25	theme	pressure	948:955	arg1	HPSEC					988:992	HPSEC	988:992	HPSEC	988:992	On high pressure size exclusion chromatography (HPSEC), the elution pattern of the acid-extracted pectins showed that severe conditions were associated with lower hydrodynamic volume.
27524274	1	26	theme	composite	84:92	arg1	design					94:99	A central composite design	74:99	A central composite design	74:99	A central composite design was employed to determine the influence of extraction conditions on production yield and chemical composition of pectin from pomegranate peels.
27524274	8	27	theme	high	943:946	arg1	chromatography					972:985	high pressure size exclusion chromatography	943:985	high pressure size exclusion chromatography (HPSEC)	943:993	On high pressure size exclusion chromatography (HPSEC), the elution pattern of the acid-extracted pectins showed that severe conditions were associated with lower hydrodynamic volume.
27524274	8	27	theme	high	943:946	arg1	HPSEC					988:992	HPSEC	988:992	HPSEC	988:992	On high pressure size exclusion chromatography (HPSEC), the elution pattern of the acid-extracted pectins showed that severe conditions were associated with lower hydrodynamic volume.
27524274	1	28	theme	chemical	190:197	arg1	composition					199:209	chemical composition	190:209	chemical composition	190:209	A central composite design was employed to determine the influence of extraction conditions on production yield and chemical composition of pectin from pomegranate peels.
27524274	6	29	theme	total	700:704	arg1	content					720:726	the total neutral sugar content	696:726	the total neutral sugar content of the extracted pectins	696:751	The uronic acid and the total neutral sugar content of the extracted pectins ranged from 377 to 755mg/g and from 161 to 326mg/g, respectively.
27524274	1	30	theme	conditions	155:164	arg1	influence					131:139	the influence	127:139	the influence of extraction conditions on production yield and chemical composition of pectin from pomegranate peels	127:242	A central composite design was employed to determine the influence of extraction conditions on production yield and chemical composition of pectin from pomegranate peels.
27524274	3	31	theme	pectin	500:505	arg1	yield					473:477	the yield	469:477	the yield of pomegranate peels pectin based on the composite design	469:535	A second-order polynomial model was developed for predicting the yield of pomegranate peels pectin based on the composite design.
27524274	3	32	theme	pomegranate	482:492	arg1	pectin					500:505	pomegranate peels pectin	482:505	pomegranate peels pectin based on the composite design	482:535	A second-order polynomial model was developed for predicting the yield of pomegranate peels pectin based on the composite design.
27524274	4	33	dep	11.0±0.2	564:571	arg1	to					561:562	to	561:562	to	561:562	Yields ranged from 6.4 to 11.0±0.2%.
27524274	0	34	theme	pectin	25:30	arg1	composition					10:20	composition	10:20	composition	10:20	Yield and composition of pectin extracted from Tunisian pomegranate peel.
27524274	0	34	theme	pectin	25:30	arg1	Yield					0:4	Yield	0:4	Yield	0:4	Yield and composition of pectin extracted from Tunisian pomegranate peel.
27524274	6	35	theme	sugar	714:718	arg1	content					720:726	the total neutral sugar content	696:726	the total neutral sugar content of the extracted pectins	696:751	The uronic acid and the total neutral sugar content of the extracted pectins ranged from 377 to 755mg/g and from 161 to 326mg/g, respectively.
27524274	1	36	theme	pectin	214:219	arg1	yield					180:184	production yield	169:184	production yield	169:184	A central composite design was employed to determine the influence of extraction conditions on production yield and chemical composition of pectin from pomegranate peels.
27524274	1	36	theme	pectin	214:219	arg1	composition					199:209	chemical composition	190:209	chemical composition	190:209	A central composite design was employed to determine the influence of extraction conditions on production yield and chemical composition of pectin from pomegranate peels.
27524274	0	37	theme	Tunisian	47:54	arg1	peel					68:71	Tunisian pomegranate peel	47:71	Tunisian pomegranate peel	47:71	Yield and composition of pectin extracted from Tunisian pomegranate peel.
27524274	1	38	from	peels	238:242	arg1	yield					180:184	production yield	169:184	production yield	169:184	A central composite design was employed to determine the influence of extraction conditions on production yield and chemical composition of pectin from pomegranate peels.
27524274	1	38	from	peels	238:242	arg1	composition					199:209	chemical composition	190:209	chemical composition	190:209	A central composite design was employed to determine the influence of extraction conditions on production yield and chemical composition of pectin from pomegranate peels.
27524274	8	39	theme	severe	1058:1063	arg1	conditions					1065:1074	severe conditions	1058:1074	severe conditions	1058:1074	On high pressure size exclusion chromatography (HPSEC), the elution pattern of the acid-extracted pectins showed that severe conditions were associated with lower hydrodynamic volume.
27524274	6	40	theme	neutral	706:712	arg1	content					720:726	the total neutral sugar content	696:726	the total neutral sugar content of the extracted pectins	696:751	The uronic acid and the total neutral sugar content of the extracted pectins ranged from 377 to 755mg/g and from 161 to 326mg/g, respectively.
27524274	8	41	theme	acid-extracted	1023:1036	arg1	pectins					1038:1044	the acid-extracted pectins	1019:1044	the acid-extracted pectins	1019:1044	On high pressure size exclusion chromatography (HPSEC), the elution pattern of the acid-extracted pectins showed that severe conditions were associated with lower hydrodynamic volume.
27524274	3	42	theme	peels	494:498	arg1	pectin					500:505	pomegranate peels pectin	482:505	pomegranate peels pectin based on the composite design	482:535	A second-order polynomial model was developed for predicting the yield of pomegranate peels pectin based on the composite design.
27524274	5	43	theme	Optimal	575:581	arg1	value					612:616	Optimal temperature, duration and pH value	575:616	Optimal temperature, duration and pH value of the extraction	575:634	Optimal temperature, duration and pH value of the extraction were 86°C, 80min and 1.7, respectively.
27524274	7	44	theme	methylation	843:853	arg1	degree					833:838	the degree	829:838	the degree of methylation	829:853	Moreover, the degree of methylation varied with the extraction conditions and the extracted pectins were low methylated.
27524274	3	45	theme	composite	520:528	arg1	design					530:535	the composite design	516:535	the composite design	516:535	A second-order polynomial model was developed for predicting the yield of pomegranate peels pectin based on the composite design.
27524274	1	46	theme	pomegranate	226:236	arg1	peels					238:242	pomegranate peels	226:242	pomegranate peels	226:242	A central composite design was employed to determine the influence of extraction conditions on production yield and chemical composition of pectin from pomegranate peels.
27524274	2	47	theme	surface	254:260	arg1	RSM					275:277	RSM	275:277	RSM	275:277	Response surface methodology (RSM) was used to quantify the integral effect of the three processing parameters (extraction duration, temperature and pH) on yield.
27524274	2	47	theme	surface	254:260	arg1	methodology					262:272	Response surface methodology	245:272	Response surface methodology (RSM)	245:278	Response surface methodology (RSM) was used to quantify the integral effect of the three processing parameters (extraction duration, temperature and pH) on yield.
27524274	2	48	theme	extraction	357:366	arg1	temperature					378:388	temperature	378:388	temperature	378:388	Response surface methodology (RSM) was used to quantify the integral effect of the three processing parameters (extraction duration, temperature and pH) on yield.
27524274	2	48	theme	extraction	357:366	arg1	duration					368:375	extraction duration	357:375	extraction duration	357:375	Response surface methodology (RSM) was used to quantify the integral effect of the three processing parameters (extraction duration, temperature and pH) on yield.
27524274	2	48	theme	extraction	357:366	arg1	pH					394:395	pH	394:395	pH	394:395	Response surface methodology (RSM) was used to quantify the integral effect of the three processing parameters (extraction duration, temperature and pH) on yield.
27524274	3	49	theme	polynomial	423:432	arg1	model					434:438	A second-order polynomial model	408:438	A second-order polynomial model	408:438	A second-order polynomial model was developed for predicting the yield of pomegranate peels pectin based on the composite design.
27524274	0	50	theme	pomegranate	56:66	arg1	peel					68:71	Tunisian pomegranate peel	47:71	Tunisian pomegranate peel	47:71	Yield and composition of pectin extracted from Tunisian pomegranate peel.
27524274	2	51	theme	Response	245:252	arg1	RSM					275:277	RSM	275:277	RSM	275:277	Response surface methodology (RSM) was used to quantify the integral effect of the three processing parameters (extraction duration, temperature and pH) on yield.
27524274	2	51	theme	Response	245:252	arg1	methodology					262:272	Response surface methodology	245:272	Response surface methodology (RSM)	245:278	Response surface methodology (RSM) was used to quantify the integral effect of the three processing parameters (extraction duration, temperature and pH) on yield.
27524274	6	52	theme	uronic	680:685	arg1	acid					687:690	The uronic acid	676:690	The uronic acid	676:690	The uronic acid and the total neutral sugar content of the extracted pectins ranged from 377 to 755mg/g and from 161 to 326mg/g, respectively.
28130889	6	0	theme	S	1190:1190	arg1	bioactivity					1172:1182	the bioactivity	1168:1182	the bioactivity of C3 S, such as the activity to induce apatite formation in simulated body fluid and to promote cell proliferation	1168:1298	Moreover, the C3 S/SA composite cements retained the bioactivity of C3 S, such as the activity to induce apatite formation in simulated body fluid and to promote cell proliferation.
28130889	5	1	dep	MPa	1113:1115	arg1	i.e.					1102:1105	i.e.	1102:1105	i.e.	1102:1105	In addition, the compressive strength of the C3 S/SA composite cement with an optimum composition could reach 54 MPa, which was significantly higher than that of C3 S (i.e., 35.3 MPa).
28130889	7	2	theme	repair	1404:1409	arg1	materials					1411:1419	bone repair materials	1399:1419	bone repair materials	1399:1419	All these results indicate that the C3 S/SA composite cements hold great promise as a new type of bone repair materials for further in vitro and in vivo studies.
28130889	3	3	with	interaction	536:546	arg1	molecules					567:575	SA molecules	564:575	SA molecules	564:575	The purpose of this study was to develop a new type of bone cements-tricalcium silicate/sodium alginate (C3 S/SA) composites by utilizing the interaction of Ca ions with SA molecules, in which an interpenetrating double-network of calcium hydrate silicate (CSH) and alginate hydrogel was formed to enhance the washout resistance, formability, injectability and compressive strength of C3 S.
28130889	7	4	theme	in	1446:1447	arg1	studies					1454:1460	further in vitro and in vivo studies	1425:1460	further in vitro and in vivo studies	1425:1460	All these results indicate that the C3 S/SA composite cements hold great promise as a new type of bone repair materials for further in vitro and in vivo studies.
28130889	6	5	dep	activity	1205:1212	arg1	promote					1273:1279	promote	1273:1279	to promote cell proliferation	1270:1298	Moreover, the C3 S/SA composite cements retained the bioactivity of C3 S, such as the activity to induce apatite formation in simulated body fluid and to promote cell proliferation.
28130889	6	5	dep	activity	1205:1212	arg1	induce					1217:1222	induce	1217:1222	to induce apatite formation in simulated body fluid	1214:1264	Moreover, the C3 S/SA composite cements retained the bioactivity of C3 S, such as the activity to induce apatite formation in simulated body fluid and to promote cell proliferation.
28130889	3	6	theme	washout	704:710	arg1	resistance					712:721	the washout resistance	700:721	the washout resistance	700:721	The purpose of this study was to develop a new type of bone cements-tricalcium silicate/sodium alginate (C3 S/SA) composites by utilizing the interaction of Ca ions with SA molecules, in which an interpenetrating double-network of calcium hydrate silicate (CSH) and alginate hydrogel was formed to enhance the washout resistance, formability, injectability and compressive strength of C3 S.
28130889	3	7	theme	SA	564:565	arg1	molecules					567:575	SA molecules	564:575	SA molecules	564:575	The purpose of this study was to develop a new type of bone cements-tricalcium silicate/sodium alginate (C3 S/SA) composites by utilizing the interaction of Ca ions with SA molecules, in which an interpenetrating double-network of calcium hydrate silicate (CSH) and alginate hydrogel was formed to enhance the washout resistance, formability, injectability and compressive strength of C3 S.
28130889	5	8	theme	composite	987:995	arg1	cement					997:1002	the C3 S/SA composite cement	975:1002	the C3 S/SA composite cement with an optimum composition	975:1030	In addition, the compressive strength of the C3 S/SA composite cement with an optimum composition could reach 54 MPa, which was significantly higher than that of C3 S (i.e., 35.3 MPa).
28130889	5	9	dep	reach	1038:1042	arg1	MPa					1113:1115	35.3 MPa	1108:1115	35.3 MPa	1108:1115	In addition, the compressive strength of the C3 S/SA composite cement with an optimum composition could reach 54 MPa, which was significantly higher than that of C3 S (i.e., 35.3 MPa).
28130889	7	10	theme	great	1368:1372	arg1	promise					1374:1380	hold great promise	1363:1380	hold great promise as a new type of bone repair materials for further in vitro and in vivo studies	1363:1460	All these results indicate that the C3 S/SA composite cements hold great promise as a new type of bone repair materials for further in vitro and in vivo studies.
28130889	3	11	theme	composites	508:517	arg1	type					441:444	a new type	435:444	a new type of bone cements-tricalcium silicate/sodium alginate (C3 S/SA) composites	435:517	The purpose of this study was to develop a new type of bone cements-tricalcium silicate/sodium alginate (C3 S/SA) composites by utilizing the interaction of Ca ions with SA molecules, in which an interpenetrating double-network of calcium hydrate silicate (CSH) and alginate hydrogel was formed to enhance the washout resistance, formability, injectability and compressive strength of C3 S.
28130889	6	12	theme	cell	1281:1284	arg1	proliferation					1286:1298	cell proliferation	1281:1298	cell proliferation	1281:1298	Moreover, the C3 S/SA composite cements retained the bioactivity of C3 S, such as the activity to induce apatite formation in simulated body fluid and to promote cell proliferation.
28130889	7	13	theme	new	1387:1389	arg1	type					1391:1394	a new type	1385:1394	a new type of bone repair materials	1385:1419	All these results indicate that the C3 S/SA composite cements hold great promise as a new type of bone repair materials for further in vitro and in vivo studies.
28130889	3	14	theme	ions	554:557	arg1	interaction					536:546	the interaction	532:546	the interaction of Ca ions with SA molecules, in which an interpenetrating double-network of calcium hydrate silicate (CSH) and alginate hydrogel was formed to enhance the washout resistance, formability, injectability and compressive strength of C3 S	532:782	The purpose of this study was to develop a new type of bone cements-tricalcium silicate/sodium alginate (C3 S/SA) composites by utilizing the interaction of Ca ions with SA molecules, in which an interpenetrating double-network of calcium hydrate silicate (CSH) and alginate hydrogel was formed to enhance the washout resistance, formability, injectability and compressive strength of C3 S.
28130889	1	15	theme	appropriate	199:209	arg1	rate					223:226	appropriate degradation rate	199:226	appropriate degradation rate	199:226	Previous studies have shown that tricalcium silicate bone cements possess good bioactivity and appropriate degradation rate.
28130889	6	16	dep	cements	1151:1157	arg1	retained					1159:1166	retained	1159:1166	cements retained the bioactivity of C3 S, such as the activity to induce apatite formation in simulated body fluid and to promote cell proliferation	1151:1298	Moreover, the C3 S/SA composite cements retained the bioactivity of C3 S, such as the activity to induce apatite formation in simulated body fluid and to promote cell proliferation.
28130889	4	17	theme	washout	816:822	arg1	resistance					824:833	the washout resistance	812:833	the washout resistance	812:833	The results confirmed that the washout resistance, formability and injectability of C3 S could indeed be greatly enhanced by the introduction of SA.
28130889	1	18	theme	Previous	104:111	arg1	studies					113:119	Previous studies	104:119	Previous studies	104:119	Previous studies have shown that tricalcium silicate bone cements possess good bioactivity and appropriate degradation rate.
28130889	1	19	theme	degradation	211:221	arg1	rate					223:226	appropriate degradation rate	199:226	appropriate degradation rate	199:226	Previous studies have shown that tricalcium silicate bone cements possess good bioactivity and appropriate degradation rate.
28130889	0	20	theme	tricalcium	10:19	arg1	bone					49:52	Bioactive tricalcium silicate/alginate composite bone	0:52	Bioactive tricalcium silicate/alginate composite bone	0:52	Bioactive tricalcium silicate/alginate composite bone cements with enhanced physicochemical properties.
28130889	5	21	theme	C3	1096:1097	arg1	S					1099:1099	C3 S	1096:1099	C3 S	1096:1099	In addition, the compressive strength of the C3 S/SA composite cement with an optimum composition could reach 54 MPa, which was significantly higher than that of C3 S (i.e., 35.3 MPa).
28130889	4	22	theme	S	872:872	arg1	injectability					852:864	injectability	852:864	injectability	852:864	The results confirmed that the washout resistance, formability and injectability of C3 S could indeed be greatly enhanced by the introduction of SA.
28130889	4	22	theme	S	872:872	arg1	formability					836:846	formability	836:846	formability	836:846	The results confirmed that the washout resistance, formability and injectability of C3 S could indeed be greatly enhanced by the introduction of SA.
28130889	4	22	theme	S	872:872	arg1	resistance					824:833	the washout resistance	812:833	the washout resistance	812:833	The results confirmed that the washout resistance, formability and injectability of C3 S could indeed be greatly enhanced by the introduction of SA.
28130889	5	23	theme	cement	997:1002	arg1	strength					963:970	the compressive strength	947:970	the compressive strength of the C3 S/SA composite cement with an optimum composition	947:1030	In addition, the compressive strength of the C3 S/SA composite cement with an optimum composition could reach 54 MPa, which was significantly higher than that of C3 S (i.e., 35.3 MPa).
28130889	7	24	theme	C3	1337:1338	arg1	composite					1345:1353	the C3 S/SA composite	1333:1353	the C3 S/SA composite	1333:1353	All these results indicate that the C3 S/SA composite cements hold great promise as a new type of bone repair materials for further in vitro and in vivo studies.
28130889	0	25	theme	Bioactive	0:8	arg1	bone					49:52	Bioactive tricalcium silicate/alginate composite bone	0:52	Bioactive tricalcium silicate/alginate composite bone	0:52	Bioactive tricalcium silicate/alginate composite bone cements with enhanced physicochemical properties.
28130889	7	26	theme	hold	1363:1366	arg1	promise					1374:1380	hold great promise	1363:1380	hold great promise as a new type of bone repair materials for further in vitro and in vivo studies	1363:1460	All these results indicate that the C3 S/SA composite cements hold great promise as a new type of bone repair materials for further in vitro and in vivo studies.
28130889	4	27	theme	C3	869:870	arg1	S					872:872	C3 S	869:872	C3 S	869:872	The results confirmed that the washout resistance, formability and injectability of C3 S could indeed be greatly enhanced by the introduction of SA.
28130889	9	28	dep	Biomater	1526:1533	arg1	237-244					1542:1548	237-244	1542:1548	237-244	1542:1548	J Biomed Mater Res Part B: Appl Biomater, 106B: 237-244, 2018.
28130889	7	29	theme	S/SA	1340:1343	arg1	composite					1345:1353	the C3 S/SA composite	1333:1353	the C3 S/SA composite	1333:1353	All these results indicate that the C3 S/SA composite cements hold great promise as a new type of bone repair materials for further in vitro and in vivo studies.
28130889	3	30	theme	bone	449:452	arg1	composites					508:517	bone cements-tricalcium silicate/sodium alginate (C3 S/SA) composites	449:517	bone cements-tricalcium silicate/sodium alginate (C3 S/SA) composites	449:517	The purpose of this study was to develop a new type of bone cements-tricalcium silicate/sodium alginate (C3 S/SA) composites by utilizing the interaction of Ca ions with SA molecules, in which an interpenetrating double-network of calcium hydrate silicate (CSH) and alginate hydrogel was formed to enhance the washout resistance, formability, injectability and compressive strength of C3 S.
28130889	0	31	theme	composite	39:47	arg1	bone					49:52	Bioactive tricalcium silicate/alginate composite bone	0:52	Bioactive tricalcium silicate/alginate composite bone	0:52	Bioactive tricalcium silicate/alginate composite bone cements with enhanced physicochemical properties.
28130889	3	32	theme	hydrate	633:639	arg1	silicate					641:648	calcium hydrate silicate	625:648	calcium hydrate silicate (CSH)	625:654	The purpose of this study was to develop a new type of bone cements-tricalcium silicate/sodium alginate (C3 S/SA) composites by utilizing the interaction of Ca ions with SA molecules, in which an interpenetrating double-network of calcium hydrate silicate (CSH) and alginate hydrogel was formed to enhance the washout resistance, formability, injectability and compressive strength of C3 S.
28130889	3	32	theme	hydrate	633:639	arg1	CSH					651:653	CSH	651:653	CSH	651:653	The purpose of this study was to develop a new type of bone cements-tricalcium silicate/sodium alginate (C3 S/SA) composites by utilizing the interaction of Ca ions with SA molecules, in which an interpenetrating double-network of calcium hydrate silicate (CSH) and alginate hydrogel was formed to enhance the washout resistance, formability, injectability and compressive strength of C3 S.
28130889	4	33	theme	SA	930:931	arg1	introduction					914:925	the introduction	910:925	the introduction of SA	910:931	The results confirmed that the washout resistance, formability and injectability of C3 S could indeed be greatly enhanced by the introduction of SA.
28130889	9	34	dep	Res	1509:1511	arg1	106B					1536:1539	106B	1536:1539	106B	1536:1539	J Biomed Mater Res Part B: Appl Biomater, 106B: 237-244, 2018.
28130889	9	34	dep	Res	1509:1511	arg1	B					1518:1518	Part B	1513:1518	J Biomed Mater Res Part B: Appl Biomater, 106B: 237-244, 2018.	1494:1555	J Biomed Mater Res Part B: Appl Biomater, 106B: 237-244, 2018.
28130889	9	34	dep	Res	1509:1511	arg1	Biomater					1526:1533	Biomater	1526:1533	Biomater	1526:1533	J Biomed Mater Res Part B: Appl Biomater, 106B: 237-244, 2018.
28130889	9	34	dep	Res	1509:1511	arg1	2018					1551:1554	2018	1551:1554	2018	1551:1554	J Biomed Mater Res Part B: Appl Biomater, 106B: 237-244, 2018.
28130889	3	35	theme	C3	499:500	arg1	composites					508:517	bone cements-tricalcium silicate/sodium alginate (C3 S/SA) composites	449:517	bone cements-tricalcium silicate/sodium alginate (C3 S/SA) composites	449:517	The purpose of this study was to develop a new type of bone cements-tricalcium silicate/sodium alginate (C3 S/SA) composites by utilizing the interaction of Ca ions with SA molecules, in which an interpenetrating double-network of calcium hydrate silicate (CSH) and alginate hydrogel was formed to enhance the washout resistance, formability, injectability and compressive strength of C3 S.
28130889	0	36	theme	silicate/alginate	21:37	arg1	bone					49:52	Bioactive tricalcium silicate/alginate composite bone	0:52	Bioactive tricalcium silicate/alginate composite bone	0:52	Bioactive tricalcium silicate/alginate composite bone cements with enhanced physicochemical properties.
28130889	3	37	theme	compressive	755:765	arg1	strength					767:774	compressive strength	755:774	compressive strength	755:774	The purpose of this study was to develop a new type of bone cements-tricalcium silicate/sodium alginate (C3 S/SA) composites by utilizing the interaction of Ca ions with SA molecules, in which an interpenetrating double-network of calcium hydrate silicate (CSH) and alginate hydrogel was formed to enhance the washout resistance, formability, injectability and compressive strength of C3 S.
28130889	3	38	theme	alginate	660:667	arg1	hydrogel					669:676	alginate hydrogel	660:676	alginate hydrogel	660:676	The purpose of this study was to develop a new type of bone cements-tricalcium silicate/sodium alginate (C3 S/SA) composites by utilizing the interaction of Ca ions with SA molecules, in which an interpenetrating double-network of calcium hydrate silicate (CSH) and alginate hydrogel was formed to enhance the washout resistance, formability, injectability and compressive strength of C3 S.
28130889	5	39	theme	compressive	951:961	arg1	strength					963:970	the compressive strength	947:970	the compressive strength of the C3 S/SA composite cement with an optimum composition	947:1030	In addition, the compressive strength of the C3 S/SA composite cement with an optimum composition could reach 54 MPa, which was significantly higher than that of C3 S (i.e., 35.3 MPa).
28130889	3	40	theme	S/SA	502:505	arg1	composites					508:517	bone cements-tricalcium silicate/sodium alginate (C3 S/SA) composites	449:517	bone cements-tricalcium silicate/sodium alginate (C3 S/SA) composites	449:517	The purpose of this study was to develop a new type of bone cements-tricalcium silicate/sodium alginate (C3 S/SA) composites by utilizing the interaction of Ca ions with SA molecules, in which an interpenetrating double-network of calcium hydrate silicate (CSH) and alginate hydrogel was formed to enhance the washout resistance, formability, injectability and compressive strength of C3 S.
28130889	3	41	theme	new	437:439	arg1	type					441:444	a new type	435:444	a new type of bone cements-tricalcium silicate/sodium alginate (C3 S/SA) composites	435:517	The purpose of this study was to develop a new type of bone cements-tricalcium silicate/sodium alginate (C3 S/SA) composites by utilizing the interaction of Ca ions with SA molecules, in which an interpenetrating double-network of calcium hydrate silicate (CSH) and alginate hydrogel was formed to enhance the washout resistance, formability, injectability and compressive strength of C3 S.
28130889	2	42	theme	clinical	371:378	arg1	applications					380:391	their clinical applications	365:391	their clinical applications	365:391	However, they also showed some weaknesses such as poor washout resistance, formability and injectability, which have seriously hindered their clinical applications.
28130889	3	43	theme	hydrogel	669:676	arg1	double-network					607:620	an interpenetrating double-network	587:620	an interpenetrating double-network of calcium hydrate silicate (CSH) and alginate hydrogel	587:676	The purpose of this study was to develop a new type of bone cements-tricalcium silicate/sodium alginate (C3 S/SA) composites by utilizing the interaction of Ca ions with SA molecules, in which an interpenetrating double-network of calcium hydrate silicate (CSH) and alginate hydrogel was formed to enhance the washout resistance, formability, injectability and compressive strength of C3 S.
28130889	6	44	theme	C3	1187:1188	arg1	activity					1205:1212	the activity to induce apatite formation in simulated body fluid and to promote cell proliferation	1201:1298	the activity to induce apatite formation in simulated body fluid and to promote cell proliferation	1201:1298	Moreover, the C3 S/SA composite cements retained the bioactivity of C3 S, such as the activity to induce apatite formation in simulated body fluid and to promote cell proliferation.
28130889	6	44	theme	C3	1187:1188	arg1	S					1190:1190	C3 S	1187:1190	C3 S	1187:1190	Moreover, the C3 S/SA composite cements retained the bioactivity of C3 S, such as the activity to induce apatite formation in simulated body fluid and to promote cell proliferation.
28130889	6	45	theme	S/SA	1136:1139	arg1	composite					1141:1149	the C3 S/SA composite	1129:1149	the C3 S/SA composite	1129:1149	Moreover, the C3 S/SA composite cements retained the bioactivity of C3 S, such as the activity to induce apatite formation in simulated body fluid and to promote cell proliferation.
28130889	3	46	theme	alginate	489:496	arg1	composites					508:517	bone cements-tricalcium silicate/sodium alginate (C3 S/SA) composites	449:517	bone cements-tricalcium silicate/sodium alginate (C3 S/SA) composites	449:517	The purpose of this study was to develop a new type of bone cements-tricalcium silicate/sodium alginate (C3 S/SA) composites by utilizing the interaction of Ca ions with SA molecules, in which an interpenetrating double-network of calcium hydrate silicate (CSH) and alginate hydrogel was formed to enhance the washout resistance, formability, injectability and compressive strength of C3 S.
28130889	3	47	theme	cements-tricalcium	454:471	arg1	composites					508:517	bone cements-tricalcium silicate/sodium alginate (C3 S/SA) composites	449:517	bone cements-tricalcium silicate/sodium alginate (C3 S/SA) composites	449:517	The purpose of this study was to develop a new type of bone cements-tricalcium silicate/sodium alginate (C3 S/SA) composites by utilizing the interaction of Ca ions with SA molecules, in which an interpenetrating double-network of calcium hydrate silicate (CSH) and alginate hydrogel was formed to enhance the washout resistance, formability, injectability and compressive strength of C3 S.
28130889	6	48	theme	body	1255:1258	arg1	fluid					1260:1264	simulated body fluid	1245:1264	simulated body fluid	1245:1264	Moreover, the C3 S/SA composite cements retained the bioactivity of C3 S, such as the activity to induce apatite formation in simulated body fluid and to promote cell proliferation.
28130889	7	49	dep	in	1433:1434	arg1	vitro					1436:1440	vitro	1436:1440	vitro	1436:1440	All these results indicate that the C3 S/SA composite cements hold great promise as a new type of bone repair materials for further in vitro and in vivo studies.
28130889	3	50	theme	calcium	625:631	arg1	silicate					641:648	calcium hydrate silicate	625:648	calcium hydrate silicate (CSH)	625:654	The purpose of this study was to develop a new type of bone cements-tricalcium silicate/sodium alginate (C3 S/SA) composites by utilizing the interaction of Ca ions with SA molecules, in which an interpenetrating double-network of calcium hydrate silicate (CSH) and alginate hydrogel was formed to enhance the washout resistance, formability, injectability and compressive strength of C3 S.
28130889	3	50	theme	calcium	625:631	arg1	CSH					651:653	CSH	651:653	CSH	651:653	The purpose of this study was to develop a new type of bone cements-tricalcium silicate/sodium alginate (C3 S/SA) composites by utilizing the interaction of Ca ions with SA molecules, in which an interpenetrating double-network of calcium hydrate silicate (CSH) and alginate hydrogel was formed to enhance the washout resistance, formability, injectability and compressive strength of C3 S.
28130889	6	51	theme	apatite	1224:1230	arg1	formation					1232:1240	apatite formation	1224:1240	apatite formation	1224:1240	Moreover, the C3 S/SA composite cements retained the bioactivity of C3 S, such as the activity to induce apatite formation in simulated body fluid and to promote cell proliferation.
28130889	0	52	theme	enhanced	67:74	arg1	properties					92:101	enhanced physicochemical properties	67:101	enhanced physicochemical properties	67:101	Bioactive tricalcium silicate/alginate composite bone cements with enhanced physicochemical properties.
28130889	6	53	theme	simulated	1245:1253	arg1	fluid					1260:1264	simulated body fluid	1245:1264	simulated body fluid	1245:1264	Moreover, the C3 S/SA composite cements retained the bioactivity of C3 S, such as the activity to induce apatite formation in simulated body fluid and to promote cell proliferation.
28130889	3	54	theme	C3	779:780	arg1	S					782:782	C3 S	779:782	C3 S	779:782	The purpose of this study was to develop a new type of bone cements-tricalcium silicate/sodium alginate (C3 S/SA) composites by utilizing the interaction of Ca ions with SA molecules, in which an interpenetrating double-network of calcium hydrate silicate (CSH) and alginate hydrogel was formed to enhance the washout resistance, formability, injectability and compressive strength of C3 S.
28130889	3	55	theme	interpenetrating	590:605	arg1	double-network					607:620	an interpenetrating double-network	587:620	an interpenetrating double-network of calcium hydrate silicate (CSH) and alginate hydrogel	587:676	The purpose of this study was to develop a new type of bone cements-tricalcium silicate/sodium alginate (C3 S/SA) composites by utilizing the interaction of Ca ions with SA molecules, in which an interpenetrating double-network of calcium hydrate silicate (CSH) and alginate hydrogel was formed to enhance the washout resistance, formability, injectability and compressive strength of C3 S.
28130889	1	56	theme	tricalcium	137:146	arg1	bone					157:160	tricalcium silicate bone	137:160	tricalcium silicate bone	137:160	Previous studies have shown that tricalcium silicate bone cements possess good bioactivity and appropriate degradation rate.
28130889	3	57	theme	S	782:782	arg1	resistance					712:721	the washout resistance	700:721	the washout resistance	700:721	The purpose of this study was to develop a new type of bone cements-tricalcium silicate/sodium alginate (C3 S/SA) composites by utilizing the interaction of Ca ions with SA molecules, in which an interpenetrating double-network of calcium hydrate silicate (CSH) and alginate hydrogel was formed to enhance the washout resistance, formability, injectability and compressive strength of C3 S.
28130889	3	57	theme	S	782:782	arg1	injectability					737:749	injectability	737:749	injectability	737:749	The purpose of this study was to develop a new type of bone cements-tricalcium silicate/sodium alginate (C3 S/SA) composites by utilizing the interaction of Ca ions with SA molecules, in which an interpenetrating double-network of calcium hydrate silicate (CSH) and alginate hydrogel was formed to enhance the washout resistance, formability, injectability and compressive strength of C3 S.
28130889	3	57	theme	S	782:782	arg1	strength					767:774	compressive strength	755:774	compressive strength	755:774	The purpose of this study was to develop a new type of bone cements-tricalcium silicate/sodium alginate (C3 S/SA) composites by utilizing the interaction of Ca ions with SA molecules, in which an interpenetrating double-network of calcium hydrate silicate (CSH) and alginate hydrogel was formed to enhance the washout resistance, formability, injectability and compressive strength of C3 S.
28130889	3	57	theme	S	782:782	arg1	formability					724:734	formability	724:734	formability	724:734	The purpose of this study was to develop a new type of bone cements-tricalcium silicate/sodium alginate (C3 S/SA) composites by utilizing the interaction of Ca ions with SA molecules, in which an interpenetrating double-network of calcium hydrate silicate (CSH) and alginate hydrogel was formed to enhance the washout resistance, formability, injectability and compressive strength of C3 S.
28130889	7	58	theme	further	1425:1431	arg1	studies					1454:1460	further in vitro and in vivo studies	1425:1460	further in vitro and in vivo studies	1425:1460	All these results indicate that the C3 S/SA composite cements hold great promise as a new type of bone repair materials for further in vitro and in vivo studies.
28130889	8	59	dep	©	1463:1463	arg1	Inc.					1489:1492	Inc.	1489:1492	Inc.	1489:1492	© 2017 Wiley Periodicals, Inc.
28130889	1	60	theme	silicate	148:155	arg1	bone					157:160	tricalcium silicate bone	137:160	tricalcium silicate bone	137:160	Previous studies have shown that tricalcium silicate bone cements possess good bioactivity and appropriate degradation rate.
28130889	3	61	theme	silicate	641:648	arg1	double-network					607:620	an interpenetrating double-network	587:620	an interpenetrating double-network of calcium hydrate silicate (CSH) and alginate hydrogel	587:676	The purpose of this study was to develop a new type of bone cements-tricalcium silicate/sodium alginate (C3 S/SA) composites by utilizing the interaction of Ca ions with SA molecules, in which an interpenetrating double-network of calcium hydrate silicate (CSH) and alginate hydrogel was formed to enhance the washout resistance, formability, injectability and compressive strength of C3 S.
28130889	7	62	theme	in	1433:1434	arg1	studies					1454:1460	further in vitro and in vivo studies	1425:1460	further in vitro and in vivo studies	1425:1460	All these results indicate that the C3 S/SA composite cements hold great promise as a new type of bone repair materials for further in vitro and in vivo studies.
28130889	5	63	theme	S/SA	982:985	arg1	cement					997:1002	the C3 S/SA composite cement	975:1002	the C3 S/SA composite cement with an optimum composition	975:1030	In addition, the compressive strength of the C3 S/SA composite cement with an optimum composition could reach 54 MPa, which was significantly higher than that of C3 S (i.e., 35.3 MPa).
28130889	5	64	theme	optimum	1012:1018	arg1	composition					1020:1030	an optimum composition	1009:1030	an optimum composition	1009:1030	In addition, the compressive strength of the C3 S/SA composite cement with an optimum composition could reach 54 MPa, which was significantly higher than that of C3 S (i.e., 35.3 MPa).
28130889	5	65	theme	C3	979:980	arg1	cement					997:1002	the C3 S/SA composite cement	975:1002	the C3 S/SA composite cement with an optimum composition	975:1030	In addition, the compressive strength of the C3 S/SA composite cement with an optimum composition could reach 54 MPa, which was significantly higher than that of C3 S (i.e., 35.3 MPa).
28130889	7	66	theme	materials	1411:1419	arg1	type					1391:1394	a new type	1385:1394	a new type of bone repair materials	1385:1419	All these results indicate that the C3 S/SA composite cements hold great promise as a new type of bone repair materials for further in vitro and in vivo studies.
28130889	2	67	theme	washout	284:290	arg1	resistance					292:301	poor washout resistance	279:301	poor washout resistance	279:301	However, they also showed some weaknesses such as poor washout resistance, formability and injectability, which have seriously hindered their clinical applications.
28130889	1	68	theme	good	178:181	arg1	bioactivity					183:193	good bioactivity	178:193	good bioactivity	178:193	Previous studies have shown that tricalcium silicate bone cements possess good bioactivity and appropriate degradation rate.
28130889	5	69	with	cement	997:1002	arg1	composition					1020:1030	an optimum composition	1009:1030	an optimum composition	1009:1030	In addition, the compressive strength of the C3 S/SA composite cement with an optimum composition could reach 54 MPa, which was significantly higher than that of C3 S (i.e., 35.3 MPa).
28130889	2	70	theme	poor	279:282	arg1	resistance					292:301	poor washout resistance	279:301	poor washout resistance	279:301	However, they also showed some weaknesses such as poor washout resistance, formability and injectability, which have seriously hindered their clinical applications.
28130889	9	71	theme	Part	1513:1516	arg1	B					1518:1518	Part B	1513:1518	J Biomed Mater Res Part B: Appl Biomater, 106B: 237-244, 2018.	1494:1555	J Biomed Mater Res Part B: Appl Biomater, 106B: 237-244, 2018.
28130889	3	72	theme	silicate/sodium	473:487	arg1	composites					508:517	bone cements-tricalcium silicate/sodium alginate (C3 S/SA) composites	449:517	bone cements-tricalcium silicate/sodium alginate (C3 S/SA) composites	449:517	The purpose of this study was to develop a new type of bone cements-tricalcium silicate/sodium alginate (C3 S/SA) composites by utilizing the interaction of Ca ions with SA molecules, in which an interpenetrating double-network of calcium hydrate silicate (CSH) and alginate hydrogel was formed to enhance the washout resistance, formability, injectability and compressive strength of C3 S.
28130889	7	73	dep	in	1446:1447	arg1	vivo					1449:1452	vivo	1449:1452	vivo	1449:1452	All these results indicate that the C3 S/SA composite cements hold great promise as a new type of bone repair materials for further in vitro and in vivo studies.
28130889	0	74	theme	physicochemical	76:90	arg1	properties					92:101	enhanced physicochemical properties	67:101	enhanced physicochemical properties	67:101	Bioactive tricalcium silicate/alginate composite bone cements with enhanced physicochemical properties.
28130889	7	75	theme	bone	1399:1402	arg1	materials					1411:1419	bone repair materials	1399:1419	bone repair materials	1399:1419	All these results indicate that the C3 S/SA composite cements hold great promise as a new type of bone repair materials for further in vitro and in vivo studies.
28130889	3	76	theme	study	414:418	arg1	purpose					398:404	The purpose	394:404	The purpose of this study	394:418	The purpose of this study was to develop a new type of bone cements-tricalcium silicate/sodium alginate (C3 S/SA) composites by utilizing the interaction of Ca ions with SA molecules, in which an interpenetrating double-network of calcium hydrate silicate (CSH) and alginate hydrogel was formed to enhance the washout resistance, formability, injectability and compressive strength of C3 S.
28130889	6	77	theme	C3	1133:1134	arg1	composite					1141:1149	the C3 S/SA composite	1129:1149	the C3 S/SA composite	1129:1149	Moreover, the C3 S/SA composite cements retained the bioactivity of C3 S, such as the activity to induce apatite formation in simulated body fluid and to promote cell proliferation.
28130889	3	78	theme	Ca	551:552	arg1	ions					554:557	Ca ions	551:557	Ca ions	551:557	The purpose of this study was to develop a new type of bone cements-tricalcium silicate/sodium alginate (C3 S/SA) composites by utilizing the interaction of Ca ions with SA molecules, in which an interpenetrating double-network of calcium hydrate silicate (CSH) and alginate hydrogel was formed to enhance the washout resistance, formability, injectability and compressive strength of C3 S.
24407857	3	0	theme	abundant	207:214	arg1	chitin					183:188	chitin	183:188	chitin	183:188	Chitosan (CS), a polysaccharide derived from chitin, the second most abundant polysaccharide, is widely used in the medical world because of its natural and nontoxic properties and its innate ability for antibacterial and hemostasis effects.
24407857	3	0	theme	abundant	207:214	arg1	polysaccharide					216:229	the second most abundant polysaccharide	191:229	the second most abundant polysaccharide	191:229	Chitosan (CS), a polysaccharide derived from chitin, the second most abundant polysaccharide, is widely used in the medical world because of its natural and nontoxic properties and its innate ability for antibacterial and hemostasis effects.
24407857	4	1	theme	green	515:519	arg1	method					544:549	a green and totally recyclable method	513:549	a green and totally recyclable method	513:549	In this study, the novel composites containing CS and cellulose (CEL) (i.e., [CEL + CS]), which we have previously synthesized using a green and totally recyclable method, were investigated for their antimicrobial activity, absorption of anticoagulated whole blood, anti-inflammatory activity through the reduction of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), and the biocompatibility with human fibroblasts.
24407857	3	2	theme	nontoxic	295:302	arg1	properties					304:313	its natural and nontoxic properties	279:313	its natural and nontoxic properties	279:313	Chitosan (CS), a polysaccharide derived from chitin, the second most abundant polysaccharide, is widely used in the medical world because of its natural and nontoxic properties and its innate ability for antibacterial and hemostasis effects.
24407857	4	3	with	activity	664:671	arg1	fibroblasts					792:802	human fibroblasts	786:802	human fibroblasts	786:802	In this study, the novel composites containing CS and cellulose (CEL) (i.e., [CEL + CS]), which we have previously synthesized using a green and totally recyclable method, were investigated for their antimicrobial activity, absorption of anticoagulated whole blood, anti-inflammatory activity through the reduction of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), and the biocompatibility with human fibroblasts.
24407857	4	4	theme	blood	639:643	arg1	activity					664:671	anti-inflammatory activity	646:671	anti-inflammatory activity through the reduction of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6)	646:753	In this study, the novel composites containing CS and cellulose (CEL) (i.e., [CEL + CS]), which we have previously synthesized using a green and totally recyclable method, were investigated for their antimicrobial activity, absorption of anticoagulated whole blood, anti-inflammatory activity through the reduction of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), and the biocompatibility with human fibroblasts.
24407857	4	4	theme	blood	639:643	arg1	biocompatibility					764:779	the biocompatibility	760:779	the biocompatibility with human fibroblasts	760:802	In this study, the novel composites containing CS and cellulose (CEL) (i.e., [CEL + CS]), which we have previously synthesized using a green and totally recyclable method, were investigated for their antimicrobial activity, absorption of anticoagulated whole blood, anti-inflammatory activity through the reduction of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), and the biocompatibility with human fibroblasts.
24407857	4	4	theme	blood	639:643	arg1	absorption					604:613	absorption	604:613	absorption of anticoagulated whole blood	604:643	In this study, the novel composites containing CS and cellulose (CEL) (i.e., [CEL + CS]), which we have previously synthesized using a green and totally recyclable method, were investigated for their antimicrobial activity, absorption of anticoagulated whole blood, anti-inflammatory activity through the reduction of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), and the biocompatibility with human fibroblasts.
24407857	4	4	theme	blood	639:643	arg1	activity					594:601	their antimicrobial activity	574:601	their antimicrobial activity	574:601	In this study, the novel composites containing CS and cellulose (CEL) (i.e., [CEL + CS]), which we have previously synthesized using a green and totally recyclable method, were investigated for their antimicrobial activity, absorption of anticoagulated whole blood, anti-inflammatory activity through the reduction of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), and the biocompatibility with human fibroblasts.
24407857	3	5	dep	antibacterial	342:354	arg1	effects					371:377	effects	371:377	effects	371:377	Chitosan (CS), a polysaccharide derived from chitin, the second most abundant polysaccharide, is widely used in the medical world because of its natural and nontoxic properties and its innate ability for antibacterial and hemostasis effects.
24407857	10	6	theme	wound	1766:1770	arg1	dressings					1772:1780	wound dressings	1766:1780	wound dressings	1766:1780	These results clearly indicate that the biodegradable, biocompatible and nontoxic [CEL + CS] composites, particularly those dried by lyophilizing, can be effectively used as a material in wound dressings.
24407857	3	7	theme	second	195:200	arg1	chitin					183:188	chitin	183:188	chitin	183:188	Chitosan (CS), a polysaccharide derived from chitin, the second most abundant polysaccharide, is widely used in the medical world because of its natural and nontoxic properties and its innate ability for antibacterial and hemostasis effects.
24407857	3	7	theme	second	195:200	arg1	polysaccharide					216:229	the second most abundant polysaccharide	191:229	the second most abundant polysaccharide	191:229	Chitosan (CS), a polysaccharide derived from chitin, the second most abundant polysaccharide, is widely used in the medical world because of its natural and nontoxic properties and its innate ability for antibacterial and hemostasis effects.
24407857	9	8	theme	air-dried	1463:1471	arg1	forms					1489:1493	both air-dried and lyophilized forms	1458:1493	both air-dried and lyophilized forms	1458:1493	The composites, in both air-dried and lyophilized forms, significantly inhibit the production of TNF-α and IL-6 by stimulated macrophages.
24407857	5	9	theme	negative	890:897	arg1	micro-organisms					899:913	both Gram positive and negative micro-organisms	867:913	both Gram positive and negative micro-organisms	867:913	The [CEL + CS] composites were found to inhibit the growth of both Gram positive and negative micro-organisms.
24407857	4	10	with	biocompatibility	764:779	arg1	fibroblasts					792:802	human fibroblasts	786:802	human fibroblasts	786:802	In this study, the novel composites containing CS and cellulose (CEL) (i.e., [CEL + CS]), which we have previously synthesized using a green and totally recyclable method, were investigated for their antimicrobial activity, absorption of anticoagulated whole blood, anti-inflammatory activity through the reduction of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), and the biocompatibility with human fibroblasts.
24407857	5	11	theme	micro-organisms	899:913	arg1	growth					857:862	the growth	853:862	the growth of both Gram positive and negative micro-organisms	853:913	The [CEL + CS] composites were found to inhibit the growth of both Gram positive and negative micro-organisms.
24407857	9	12	theme	TNF-α	1536:1540	arg1	production					1522:1531	the production	1518:1531	the production of TNF-α and IL-6	1518:1549	The composites, in both air-dried and lyophilized forms, significantly inhibit the production of TNF-α and IL-6 by stimulated macrophages.
24407857	4	13	theme	factor-α	713:720	arg1	reduction					685:693	the reduction	681:693	the reduction of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6)	681:753	In this study, the novel composites containing CS and cellulose (CEL) (i.e., [CEL + CS]), which we have previously synthesized using a green and totally recyclable method, were investigated for their antimicrobial activity, absorption of anticoagulated whole blood, anti-inflammatory activity through the reduction of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), and the biocompatibility with human fibroblasts.
24407857	3	14	used	used	242:245	arg2	CS					148:149	CS	148:149	CS	148:149	Chitosan (CS), a polysaccharide derived from chitin, the second most abundant polysaccharide, is widely used in the medical world because of its natural and nontoxic properties and its innate ability for antibacterial and hemostasis effects.
24407857	3	14	used	used	242:245	arg2	Chitosan					138:145	Chitosan	138:145	Chitosan (CS)	138:150	Chitosan (CS), a polysaccharide derived from chitin, the second most abundant polysaccharide, is widely used in the medical world because of its natural and nontoxic properties and its innate ability for antibacterial and hemostasis effects.
24407857	3	14	used	used	242:245	arg2	polysaccharide					155:168	a polysaccharide	153:168	a polysaccharide derived from chitin, the second most abundant polysaccharide	153:229	Chitosan (CS), a polysaccharide derived from chitin, the second most abundant polysaccharide, is widely used in the medical world because of its natural and nontoxic properties and its innate ability for antibacterial and hemostasis effects.
24407857	8	15	dep	rate	1380:1383	arg1	the					1371:1373	the	1371:1373	the	1371:1373	They effectively absorb blood, and at the same rate and volume as commercially available wound dressings.
24407857	10	16	theme	biodegradable	1618:1630	arg1	composites					1671:1680	the biodegradable, biocompatible and nontoxic [CEL + CS] composites	1614:1680	the biodegradable, biocompatible and nontoxic [CEL + CS] composites	1614:1680	These results clearly indicate that the biodegradable, biocompatible and nontoxic [CEL + CS] composites, particularly those dried by lyophilizing, can be effectively used as a material in wound dressings.
24407857	10	16	theme	biodegradable	1618:1630	arg1	those					1696:1700	those	1696:1700	those	1696:1700	These results clearly indicate that the biodegradable, biocompatible and nontoxic [CEL + CS] composites, particularly those dried by lyophilizing, can be effectively used as a material in wound dressings.
24407857	10	16	theme	biodegradable	1618:1630	arg1	material					1754:1761	a material	1752:1761	a material in wound dressings	1752:1780	These results clearly indicate that the biodegradable, biocompatible and nontoxic [CEL + CS] composites, particularly those dried by lyophilizing, can be effectively used as a material in wound dressings.
24407857	3	17	theme	innate	323:328	arg1	ability					330:336	its innate ability	319:336	its innate ability for antibacterial and hemostasis effects	319:377	Chitosan (CS), a polysaccharide derived from chitin, the second most abundant polysaccharide, is widely used in the medical world because of its natural and nontoxic properties and its innate ability for antibacterial and hemostasis effects.
24407857	10	18	from	material	1754:1761	arg1	dressings					1772:1780	wound dressings	1766:1780	wound dressings	1766:1780	These results clearly indicate that the biodegradable, biocompatible and nontoxic [CEL + CS] composites, particularly those dried by lyophilizing, can be effectively used as a material in wound dressings.
24407857	6	19	theme	regenerated	934:944	arg1	material					972:979	the regenerated 100% lyophilized chitosan material	930:979	the regenerated 100% lyophilized chitosan material	930:979	For examples, the regenerated 100% lyophilized chitosan material was found to reduce growth of Escherichia coli (ATCC 8739 and vancomycin resistant Enterococcus faecalis (ATCC 51299) by 78, 36, and 64%, respectively.
24407857	6	20	theme	lyophilized	951:961	arg1	material					972:979	the regenerated 100% lyophilized chitosan material	930:979	the regenerated 100% lyophilized chitosan material	930:979	For examples, the regenerated 100% lyophilized chitosan material was found to reduce growth of Escherichia coli (ATCC 8739 and vancomycin resistant Enterococcus faecalis (ATCC 51299) by 78, 36, and 64%, respectively.
24407857	6	21	theme	coli	1023:1026	arg1	growth					1001:1006	growth	1001:1006	growth of Escherichia coli (ATCC 8739 and vancomycin resistant Enterococcus faecalis (ATCC 51299)	1001:1097	For examples, the regenerated 100% lyophilized chitosan material was found to reduce growth of Escherichia coli (ATCC 8739 and vancomycin resistant Enterococcus faecalis (ATCC 51299) by 78, 36, and 64%, respectively.
24407857	10	22	theme	biocompatible	1633:1645	arg1	composites					1671:1680	the biodegradable, biocompatible and nontoxic [CEL + CS] composites	1614:1680	the biodegradable, biocompatible and nontoxic [CEL + CS] composites	1614:1680	These results clearly indicate that the biodegradable, biocompatible and nontoxic [CEL + CS] composites, particularly those dried by lyophilizing, can be effectively used as a material in wound dressings.
24407857	10	22	theme	biocompatible	1633:1645	arg1	those					1696:1700	those	1696:1700	those	1696:1700	These results clearly indicate that the biodegradable, biocompatible and nontoxic [CEL + CS] composites, particularly those dried by lyophilizing, can be effectively used as a material in wound dressings.
24407857	10	22	theme	biocompatible	1633:1645	arg1	material					1754:1761	a material	1752:1761	a material in wound dressings	1752:1780	These results clearly indicate that the biodegradable, biocompatible and nontoxic [CEL + CS] composites, particularly those dried by lyophilizing, can be effectively used as a material in wound dressings.
24407857	7	23	theme	composites	1321:1330	arg1	presence					1305:1312	the presence	1301:1312	the presence of the composites	1301:1330	The composites are nontoxic to fibroblasts; that is, fibroblasts, which are critical to the formation of connective tissue matrix were found to grow and proliferate in the presence of the composites.
24407857	9	24	theme	IL-6	1546:1549	arg1	production					1522:1531	the production	1518:1531	the production of TNF-α and IL-6	1518:1549	The composites, in both air-dried and lyophilized forms, significantly inhibit the production of TNF-α and IL-6 by stimulated macrophages.
24407857	5	25	theme	[CEL + CS	809:817	arg1	composites					820:829	The [CEL + CS] composites	805:829	The [CEL + CS] composites	805:829	The [CEL + CS] composites were found to inhibit the growth of both Gram positive and negative micro-organisms.
24407857	8	26	theme	same	1375:1378	arg1	rate					1380:1383	same rate	1375:1383	same rate	1375:1383	They effectively absorb blood, and at the same rate and volume as commercially available wound dressings.
24407857	8	27	theme	available	1412:1420	arg1	dressings					1428:1436	commercially available wound dressings	1399:1436	commercially available wound dressings	1399:1436	They effectively absorb blood, and at the same rate and volume as commercially available wound dressings.
24407857	4	28	theme	human	786:790	arg1	fibroblasts					792:802	human fibroblasts	786:802	human fibroblasts	786:802	In this study, the novel composites containing CS and cellulose (CEL) (i.e., [CEL + CS]), which we have previously synthesized using a green and totally recyclable method, were investigated for their antimicrobial activity, absorption of anticoagulated whole blood, anti-inflammatory activity through the reduction of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), and the biocompatibility with human fibroblasts.
24407857	5	29	theme	Gram	872:875	arg1	micro-organisms					899:913	both Gram positive and negative micro-organisms	867:913	both Gram positive and negative micro-organisms	867:913	The [CEL + CS] composites were found to inhibit the growth of both Gram positive and negative micro-organisms.
24407857	4	30	with	absorption	604:613	arg1	fibroblasts					792:802	human fibroblasts	786:802	human fibroblasts	786:802	In this study, the novel composites containing CS and cellulose (CEL) (i.e., [CEL + CS]), which we have previously synthesized using a green and totally recyclable method, were investigated for their antimicrobial activity, absorption of anticoagulated whole blood, anti-inflammatory activity through the reduction of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), and the biocompatibility with human fibroblasts.
24407857	0	31	theme	wound	33:37	arg1	material					48:55	wound dressing material	33:55	wound dressing material	33:55	Chitosan-cellulose composite for wound dressing material.
24407857	2	32	theme	Antimicrobial	66:78	arg1	activity					80:87	Antimicrobial activity	66:87	Antimicrobial activity	66:87	Antimicrobial activity, blood absorption ability, and biocompatibility.
24407857	3	33	theme	medical	254:260	arg1	world					262:266	the medical world	250:266	the medical world	250:266	Chitosan (CS), a polysaccharide derived from chitin, the second most abundant polysaccharide, is widely used in the medical world because of its natural and nontoxic properties and its innate ability for antibacterial and hemostasis effects.
24407857	4	34	theme	necrosis	704:711	arg1	TNF-α					723:727	TNF-α	723:727	TNF-α	723:727	In this study, the novel composites containing CS and cellulose (CEL) (i.e., [CEL + CS]), which we have previously synthesized using a green and totally recyclable method, were investigated for their antimicrobial activity, absorption of anticoagulated whole blood, anti-inflammatory activity through the reduction of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), and the biocompatibility with human fibroblasts.
24407857	4	34	theme	necrosis	704:711	arg1	factor-α					713:720	tumor necrosis factor-α	698:720	tumor necrosis factor-α (TNF-α)	698:728	In this study, the novel composites containing CS and cellulose (CEL) (i.e., [CEL + CS]), which we have previously synthesized using a green and totally recyclable method, were investigated for their antimicrobial activity, absorption of anticoagulated whole blood, anti-inflammatory activity through the reduction of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), and the biocompatibility with human fibroblasts.
24407857	3	35	attach	derived	170:176	arg1	chitin					183:188	chitin	183:188	chitin	183:188	Chitosan (CS), a polysaccharide derived from chitin, the second most abundant polysaccharide, is widely used in the medical world because of its natural and nontoxic properties and its innate ability for antibacterial and hemostasis effects.
24407857	3	35	attach	derived	170:176	arg1	polysaccharide					216:229	the second most abundant polysaccharide	191:229	the second most abundant polysaccharide	191:229	Chitosan (CS), a polysaccharide derived from chitin, the second most abundant polysaccharide, is widely used in the medical world because of its natural and nontoxic properties and its innate ability for antibacterial and hemostasis effects.
24407857	3	35	attach	derived	170:176	arg2	Chitosan					138:145	Chitosan	138:145	Chitosan (CS)	138:150	Chitosan (CS), a polysaccharide derived from chitin, the second most abundant polysaccharide, is widely used in the medical world because of its natural and nontoxic properties and its innate ability for antibacterial and hemostasis effects.
24407857	3	35	attach	derived	170:176	arg2	polysaccharide					155:168	a polysaccharide	153:168	a polysaccharide derived from chitin, the second most abundant polysaccharide	153:229	Chitosan (CS), a polysaccharide derived from chitin, the second most abundant polysaccharide, is widely used in the medical world because of its natural and nontoxic properties and its innate ability for antibacterial and hemostasis effects.
24407857	10	36	theme	[CEL + CS	1660:1668	arg1	composites					1671:1680	the biodegradable, biocompatible and nontoxic [CEL + CS] composites	1614:1680	the biodegradable, biocompatible and nontoxic [CEL + CS] composites	1614:1680	These results clearly indicate that the biodegradable, biocompatible and nontoxic [CEL + CS] composites, particularly those dried by lyophilizing, can be effectively used as a material in wound dressings.
24407857	10	36	theme	[CEL + CS	1660:1668	arg1	those					1696:1700	those	1696:1700	those	1696:1700	These results clearly indicate that the biodegradable, biocompatible and nontoxic [CEL + CS] composites, particularly those dried by lyophilizing, can be effectively used as a material in wound dressings.
24407857	10	36	theme	[CEL + CS	1660:1668	arg1	material					1754:1761	a material	1752:1761	a material in wound dressings	1752:1780	These results clearly indicate that the biodegradable, biocompatible and nontoxic [CEL + CS] composites, particularly those dried by lyophilizing, can be effectively used as a material in wound dressings.
24407857	4	37	theme	tumor	698:702	arg1	TNF-α					723:727	TNF-α	723:727	TNF-α	723:727	In this study, the novel composites containing CS and cellulose (CEL) (i.e., [CEL + CS]), which we have previously synthesized using a green and totally recyclable method, were investigated for their antimicrobial activity, absorption of anticoagulated whole blood, anti-inflammatory activity through the reduction of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), and the biocompatibility with human fibroblasts.
24407857	4	37	theme	tumor	698:702	arg1	factor-α					713:720	tumor necrosis factor-α	698:720	tumor necrosis factor-α (TNF-α)	698:728	In this study, the novel composites containing CS and cellulose (CEL) (i.e., [CEL + CS]), which we have previously synthesized using a green and totally recyclable method, were investigated for their antimicrobial activity, absorption of anticoagulated whole blood, anti-inflammatory activity through the reduction of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), and the biocompatibility with human fibroblasts.
24407857	8	38	theme	wound	1422:1426	arg1	dressings					1428:1436	commercially available wound dressings	1399:1436	commercially available wound dressings	1399:1436	They effectively absorb blood, and at the same rate and volume as commercially available wound dressings.
24407857	4	39	theme	antimicrobial	580:592	arg1	activity					594:601	their antimicrobial activity	574:601	their antimicrobial activity	574:601	In this study, the novel composites containing CS and cellulose (CEL) (i.e., [CEL + CS]), which we have previously synthesized using a green and totally recyclable method, were investigated for their antimicrobial activity, absorption of anticoagulated whole blood, anti-inflammatory activity through the reduction of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), and the biocompatibility with human fibroblasts.
24407857	4	40	contain	containing	416:425	arg2	cellulose					434:442	cellulose	434:442	cellulose	434:442	In this study, the novel composites containing CS and cellulose (CEL) (i.e., [CEL + CS]), which we have previously synthesized using a green and totally recyclable method, were investigated for their antimicrobial activity, absorption of anticoagulated whole blood, anti-inflammatory activity through the reduction of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), and the biocompatibility with human fibroblasts.
24407857	4	40	contain	containing	416:425	arg2	CS					427:428	CS	427:428	CS	427:428	In this study, the novel composites containing CS and cellulose (CEL) (i.e., [CEL + CS]), which we have previously synthesized using a green and totally recyclable method, were investigated for their antimicrobial activity, absorption of anticoagulated whole blood, anti-inflammatory activity through the reduction of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), and the biocompatibility with human fibroblasts.
24407857	4	40	contain	containing	416:425	arg1	composites					405:414	the novel composites	395:414	the novel composites	395:414	In this study, the novel composites containing CS and cellulose (CEL) (i.e., [CEL + CS]), which we have previously synthesized using a green and totally recyclable method, were investigated for their antimicrobial activity, absorption of anticoagulated whole blood, anti-inflammatory activity through the reduction of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), and the biocompatibility with human fibroblasts.
24407857	4	40	contain	containing	416:425	arg2	[CEL + CS					457:465	[CEL + CS	457:465	[CEL + CS	457:465	In this study, the novel composites containing CS and cellulose (CEL) (i.e., [CEL + CS]), which we have previously synthesized using a green and totally recyclable method, were investigated for their antimicrobial activity, absorption of anticoagulated whole blood, anti-inflammatory activity through the reduction of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), and the biocompatibility with human fibroblasts.
24407857	4	40	contain	containing	416:425	arg2	CEL					445:447	CEL	445:447	CEL	445:447	In this study, the novel composites containing CS and cellulose (CEL) (i.e., [CEL + CS]), which we have previously synthesized using a green and totally recyclable method, were investigated for their antimicrobial activity, absorption of anticoagulated whole blood, anti-inflammatory activity through the reduction of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), and the biocompatibility with human fibroblasts.
24407857	0	41	theme	dressing	39:46	arg1	material					48:55	wound dressing material	33:55	wound dressing material	33:55	Chitosan-cellulose composite for wound dressing material.
24407857	6	42	dep	coli	1023:1026	arg1	8739					1034:1037	ATCC 8739	1029:1037	Escherichia coli (ATCC 8739	1011:1037	For examples, the regenerated 100% lyophilized chitosan material was found to reduce growth of Escherichia coli (ATCC 8739 and vancomycin resistant Enterococcus faecalis (ATCC 51299) by 78, 36, and 64%, respectively.
24407857	10	43	used	used	1744:1747	arg2	composites					1671:1680	the biodegradable, biocompatible and nontoxic [CEL + CS] composites	1614:1680	the biodegradable, biocompatible and nontoxic [CEL + CS] composites	1614:1680	These results clearly indicate that the biodegradable, biocompatible and nontoxic [CEL + CS] composites, particularly those dried by lyophilizing, can be effectively used as a material in wound dressings.
24407857	10	43	used	used	1744:1747	arg2	material					1754:1761	a material	1752:1761	a material in wound dressings	1752:1780	These results clearly indicate that the biodegradable, biocompatible and nontoxic [CEL + CS] composites, particularly those dried by lyophilizing, can be effectively used as a material in wound dressings.
24407857	10	43	used	used	1744:1747	arg2	those					1696:1700	those	1696:1700	those	1696:1700	These results clearly indicate that the biodegradable, biocompatible and nontoxic [CEL + CS] composites, particularly those dried by lyophilizing, can be effectively used as a material in wound dressings.
24407857	2	44	theme	absorption	96:105	arg1	ability					107:113	blood absorption ability	90:113	blood absorption ability	90:113	Antimicrobial activity, blood absorption ability, and biocompatibility.
24407857	4	45	with	activity	594:601	arg1	fibroblasts					792:802	human fibroblasts	786:802	human fibroblasts	786:802	In this study, the novel composites containing CS and cellulose (CEL) (i.e., [CEL + CS]), which we have previously synthesized using a green and totally recyclable method, were investigated for their antimicrobial activity, absorption of anticoagulated whole blood, anti-inflammatory activity through the reduction of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), and the biocompatibility with human fibroblasts.
24407857	7	46	theme	tissue	1249:1254	arg1	matrix					1256:1261	connective tissue matrix	1238:1261	connective tissue matrix	1238:1261	The composites are nontoxic to fibroblasts; that is, fibroblasts, which are critical to the formation of connective tissue matrix were found to grow and proliferate in the presence of the composites.
24407857	2	47	theme	blood	90:94	arg1	ability					107:113	blood absorption ability	90:113	blood absorption ability	90:113	Antimicrobial activity, blood absorption ability, and biocompatibility.
24407857	3	48	theme	natural	283:289	arg1	properties					304:313	its natural and nontoxic properties	279:313	its natural and nontoxic properties	279:313	Chitosan (CS), a polysaccharide derived from chitin, the second most abundant polysaccharide, is widely used in the medical world because of its natural and nontoxic properties and its innate ability for antibacterial and hemostasis effects.
24407857	10	49	theme	nontoxic	1651:1658	arg1	composites					1671:1680	the biodegradable, biocompatible and nontoxic [CEL + CS] composites	1614:1680	the biodegradable, biocompatible and nontoxic [CEL + CS] composites	1614:1680	These results clearly indicate that the biodegradable, biocompatible and nontoxic [CEL + CS] composites, particularly those dried by lyophilizing, can be effectively used as a material in wound dressings.
24407857	10	49	theme	nontoxic	1651:1658	arg1	those					1696:1700	those	1696:1700	those	1696:1700	These results clearly indicate that the biodegradable, biocompatible and nontoxic [CEL + CS] composites, particularly those dried by lyophilizing, can be effectively used as a material in wound dressings.
24407857	10	49	theme	nontoxic	1651:1658	arg1	material					1754:1761	a material	1752:1761	a material in wound dressings	1752:1780	These results clearly indicate that the biodegradable, biocompatible and nontoxic [CEL + CS] composites, particularly those dried by lyophilizing, can be effectively used as a material in wound dressings.
24407857	7	50	theme	matrix	1256:1261	arg1	formation					1225:1233	the formation	1221:1233	the formation of connective tissue matrix	1221:1261	The composites are nontoxic to fibroblasts; that is, fibroblasts, which are critical to the formation of connective tissue matrix were found to grow and proliferate in the presence of the composites.
24407857	4	51	theme	anti-inflammatory	646:662	arg1	activity					664:671	anti-inflammatory activity	646:671	anti-inflammatory activity through the reduction of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6)	646:753	In this study, the novel composites containing CS and cellulose (CEL) (i.e., [CEL + CS]), which we have previously synthesized using a green and totally recyclable method, were investigated for their antimicrobial activity, absorption of anticoagulated whole blood, anti-inflammatory activity through the reduction of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), and the biocompatibility with human fibroblasts.
24407857	9	52	from	composites	1443:1452	arg1	forms					1489:1493	both air-dried and lyophilized forms	1458:1493	both air-dried and lyophilized forms	1458:1493	The composites, in both air-dried and lyophilized forms, significantly inhibit the production of TNF-α and IL-6 by stimulated macrophages.
24407857	9	53	theme	stimulated	1554:1563	arg1	macrophages					1565:1575	stimulated macrophages	1554:1575	stimulated macrophages	1554:1575	The composites, in both air-dried and lyophilized forms, significantly inhibit the production of TNF-α and IL-6 by stimulated macrophages.
24407857	9	54	theme	lyophilized	1477:1487	arg1	forms					1489:1493	both air-dried and lyophilized forms	1458:1493	both air-dried and lyophilized forms	1458:1493	The composites, in both air-dried and lyophilized forms, significantly inhibit the production of TNF-α and IL-6 by stimulated macrophages.
24407857	4	55	theme	interleukin-6	734:746	arg1	reduction					685:693	the reduction	681:693	the reduction of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6)	681:753	In this study, the novel composites containing CS and cellulose (CEL) (i.e., [CEL + CS]), which we have previously synthesized using a green and totally recyclable method, were investigated for their antimicrobial activity, absorption of anticoagulated whole blood, anti-inflammatory activity through the reduction of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), and the biocompatibility with human fibroblasts.
24407857	5	56	theme	positive	877:884	arg1	micro-organisms					899:913	both Gram positive and negative micro-organisms	867:913	both Gram positive and negative micro-organisms	867:913	The [CEL + CS] composites were found to inhibit the growth of both Gram positive and negative micro-organisms.
24407857	6	57	theme	chitosan	963:970	arg1	material					972:979	the regenerated 100% lyophilized chitosan material	930:979	the regenerated 100% lyophilized chitosan material	930:979	For examples, the regenerated 100% lyophilized chitosan material was found to reduce growth of Escherichia coli (ATCC 8739 and vancomycin resistant Enterococcus faecalis (ATCC 51299) by 78, 36, and 64%, respectively.
24407857	7	58	theme	connective	1238:1247	arg1	matrix					1256:1261	connective tissue matrix	1238:1261	connective tissue matrix	1238:1261	The composites are nontoxic to fibroblasts; that is, fibroblasts, which are critical to the formation of connective tissue matrix were found to grow and proliferate in the presence of the composites.
24407857	6	59	theme	ATCC	1029:1032	arg1	8739					1034:1037	ATCC 8739	1029:1037	Escherichia coli (ATCC 8739	1011:1037	For examples, the regenerated 100% lyophilized chitosan material was found to reduce growth of Escherichia coli (ATCC 8739 and vancomycin resistant Enterococcus faecalis (ATCC 51299) by 78, 36, and 64%, respectively.
24407857	4	60	theme	recyclable	533:542	arg1	method					544:549	a green and totally recyclable method	513:549	a green and totally recyclable method	513:549	In this study, the novel composites containing CS and cellulose (CEL) (i.e., [CEL + CS]), which we have previously synthesized using a green and totally recyclable method, were investigated for their antimicrobial activity, absorption of anticoagulated whole blood, anti-inflammatory activity through the reduction of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), and the biocompatibility with human fibroblasts.
24407857	4	61	theme	novel	399:403	arg1	composites					405:414	the novel composites	395:414	the novel composites	395:414	In this study, the novel composites containing CS and cellulose (CEL) (i.e., [CEL + CS]), which we have previously synthesized using a green and totally recyclable method, were investigated for their antimicrobial activity, absorption of anticoagulated whole blood, anti-inflammatory activity through the reduction of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), and the biocompatibility with human fibroblasts.
24407857	4	62	theme	whole	633:637	arg1	blood					639:643	anticoagulated whole blood	618:643	anticoagulated whole blood	618:643	In this study, the novel composites containing CS and cellulose (CEL) (i.e., [CEL + CS]), which we have previously synthesized using a green and totally recyclable method, were investigated for their antimicrobial activity, absorption of anticoagulated whole blood, anti-inflammatory activity through the reduction of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), and the biocompatibility with human fibroblasts.
24407857	6	63	theme	%	949:949	arg1	material					972:979	the regenerated 100% lyophilized chitosan material	930:979	the regenerated 100% lyophilized chitosan material	930:979	For examples, the regenerated 100% lyophilized chitosan material was found to reduce growth of Escherichia coli (ATCC 8739 and vancomycin resistant Enterococcus faecalis (ATCC 51299) by 78, 36, and 64%, respectively.
24407857	6	64	theme	faecalis	1077:1084	arg1	growth					1001:1006	growth	1001:1006	growth of Escherichia coli (ATCC 8739 and vancomycin resistant Enterococcus faecalis (ATCC 51299)	1001:1097	For examples, the regenerated 100% lyophilized chitosan material was found to reduce growth of Escherichia coli (ATCC 8739 and vancomycin resistant Enterococcus faecalis (ATCC 51299) by 78, 36, and 64%, respectively.
24407857	6	65	theme	100	946:948	arg1	%					949:949	%	949:949	%	949:949	For examples, the regenerated 100% lyophilized chitosan material was found to reduce growth of Escherichia coli (ATCC 8739 and vancomycin resistant Enterococcus faecalis (ATCC 51299) by 78, 36, and 64%, respectively.
24407857	4	66	theme	anticoagulated	618:631	arg1	blood					639:643	anticoagulated whole blood	618:643	anticoagulated whole blood	618:643	In this study, the novel composites containing CS and cellulose (CEL) (i.e., [CEL + CS]), which we have previously synthesized using a green and totally recyclable method, were investigated for their antimicrobial activity, absorption of anticoagulated whole blood, anti-inflammatory activity through the reduction of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), and the biocompatibility with human fibroblasts.
24407857	4	67	dep	[CEL + CS	457:465	arg1	i.e.					451:454	i.e.	451:454	i.e.	451:454	In this study, the novel composites containing CS and cellulose (CEL) (i.e., [CEL + CS]), which we have previously synthesized using a green and totally recyclable method, were investigated for their antimicrobial activity, absorption of anticoagulated whole blood, anti-inflammatory activity through the reduction of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), and the biocompatibility with human fibroblasts.
27871790	2	0	from	var	313:315	arg1	polysaccharides					257:271	acidic- and alkali-extractable mycelia zinc polysaccharides	213:271	acidic- and alkali-extractable mycelia zinc polysaccharides (AcMZPS, AlMZPS) from Pleurotus eryngii var	213:315	The aims of this work were designed to investigate the hepatoprotective and antioxidant effects of acidic- and alkali-extractable mycelia zinc polysaccharides (AcMZPS, AlMZPS) from Pleurotus eryngii var.
27871790	2	0	from	var	313:315	arg1	effects					202:208	the hepatoprotective and antioxidant effects	165:208	the hepatoprotective and antioxidant effects of acidic- and alkali-extractable mycelia zinc polysaccharides (AcMZPS, AlMZPS) from Pleurotus eryngii var	165:315	The aims of this work were designed to investigate the hepatoprotective and antioxidant effects of acidic- and alkali-extractable mycelia zinc polysaccharides (AcMZPS, AlMZPS) from Pleurotus eryngii var.
27871790	2	0	from	var	313:315	arg1	AlMZPS					282:287	AlMZPS	282:287	AlMZPS	282:287	The aims of this work were designed to investigate the hepatoprotective and antioxidant effects of acidic- and alkali-extractable mycelia zinc polysaccharides (AcMZPS, AlMZPS) from Pleurotus eryngii var.
27871790	0	1	theme	eryngii	89:95	arg1	var					97:99	Pleurotus eryngii var	79:99	Pleurotus eryngii var	79:99	Antioxidant and anti-hyperlipidemic effects of mycelia zinc polysaccharides by Pleurotus eryngii var.
27871790	2	2	theme	eryngii	305:311	arg1	var					313:315	Pleurotus eryngii var	295:315	Pleurotus eryngii var	295:315	The aims of this work were designed to investigate the hepatoprotective and antioxidant effects of acidic- and alkali-extractable mycelia zinc polysaccharides (AcMZPS, AlMZPS) from Pleurotus eryngii var.
27871790	2	3	from	effects	202:208	arg1	var					313:315	Pleurotus eryngii var	295:315	Pleurotus eryngii var	295:315	The aims of this work were designed to investigate the hepatoprotective and antioxidant effects of acidic- and alkali-extractable mycelia zinc polysaccharides (AcMZPS, AlMZPS) from Pleurotus eryngii var.
27871790	4	4	theme	LDL-C	542:546	arg1	levels					532:537	the levels	528:537	the levels of LDL-C, VLDL-C, TC, TG, ALT, AST, ALP, MDA and LPO	528:590	The in vivo experiments demonstrated that both AcMZPS and AlMZPS had potential hepatoprotective effects by significantly decreasing the levels of LDL-C, VLDL-C, TC, TG, ALT, AST, ALP, MDA and LPO, and remarkably increasing the HDL-C, SOD, GSH-Px, and CAT in serum lipid/liver homogenate, respectively.
27871790	5	5	theme	antioxidant	945:955	arg1	activity					957:964	the in vitro antioxidant activity	932:964	the in vitro antioxidant activity	932:964	In addition, four polysaccharide fractions of AcMZPS-1, AcMZPS-2, AlMZPS-1, and AlMZPS-2, purified from AcMZPS and AlMZPS using DEAE chromatography, respectively, were subjected to monosaccharide composition analysis and valuated for the in vitro antioxidant activity.
27871790	2	6	theme	Pleurotus	295:303	arg1	var					313:315	Pleurotus eryngii var	295:315	Pleurotus eryngii var	295:315	The aims of this work were designed to investigate the hepatoprotective and antioxidant effects of acidic- and alkali-extractable mycelia zinc polysaccharides (AcMZPS, AlMZPS) from Pleurotus eryngii var.
27871790	5	7	theme	polysaccharide	716:729	arg1	fractions					731:739	four polysaccharide fractions	711:739	four polysaccharide fractions	711:739	In addition, four polysaccharide fractions of AcMZPS-1, AcMZPS-2, AlMZPS-1, and AlMZPS-2, purified from AcMZPS and AlMZPS using DEAE chromatography, respectively, were subjected to monosaccharide composition analysis and valuated for the in vitro antioxidant activity.
27871790	6	8	theme	non-alcoholic	1153:1165	arg1	liver					1173:1177	non-alcoholic fatty liver	1153:1177	non-alcoholic fatty liver	1153:1177	The results obtained in present study suggested that AcMZPS, AlMZPS and their purified fractions could be used as functional foods and natural drugs in preventing the hyperlipidemia and non-alcoholic fatty liver.
27871790	4	9	from	CAT	647:649	arg1	homogenate					672:681	serum lipid/liver homogenate	654:681	serum lipid/liver homogenate	654:681	The in vivo experiments demonstrated that both AcMZPS and AlMZPS had potential hepatoprotective effects by significantly decreasing the levels of LDL-C, VLDL-C, TC, TG, ALT, AST, ALP, MDA and LPO, and remarkably increasing the HDL-C, SOD, GSH-Px, and CAT in serum lipid/liver homogenate, respectively.
27871790	5	10	theme	DEAE	826:829	arg1	chromatography					831:844	DEAE chromatography	826:844	DEAE chromatography	826:844	In addition, four polysaccharide fractions of AcMZPS-1, AcMZPS-2, AlMZPS-1, and AlMZPS-2, purified from AcMZPS and AlMZPS using DEAE chromatography, respectively, were subjected to monosaccharide composition analysis and valuated for the in vitro antioxidant activity.
27871790	5	11	theme	AlMZPS-2	778:785	arg1	fractions					731:739	four polysaccharide fractions	711:739	four polysaccharide fractions	711:739	In addition, four polysaccharide fractions of AcMZPS-1, AcMZPS-2, AlMZPS-1, and AlMZPS-2, purified from AcMZPS and AlMZPS using DEAE chromatography, respectively, were subjected to monosaccharide composition analysis and valuated for the in vitro antioxidant activity.
27871790	5	12	theme	AcMZPS-2	754:761	arg1	fractions					731:739	four polysaccharide fractions	711:739	four polysaccharide fractions	711:739	In addition, four polysaccharide fractions of AcMZPS-1, AcMZPS-2, AlMZPS-1, and AlMZPS-2, purified from AcMZPS and AlMZPS using DEAE chromatography, respectively, were subjected to monosaccharide composition analysis and valuated for the in vitro antioxidant activity.
27871790	0	13	theme	Pleurotus	79:87	arg1	var					97:99	Pleurotus eryngii var	79:99	Pleurotus eryngii var	79:99	Antioxidant and anti-hyperlipidemic effects of mycelia zinc polysaccharides by Pleurotus eryngii var.
27871790	6	14	theme	purified	1045:1052	arg1	AlMZPS					1028:1033	AlMZPS	1028:1033	AlMZPS	1028:1033	The results obtained in present study suggested that AcMZPS, AlMZPS and their purified fractions could be used as functional foods and natural drugs in preventing the hyperlipidemia and non-alcoholic fatty liver.
27871790	6	14	theme	purified	1045:1052	arg1	AcMZPS					1020:1025	AcMZPS	1020:1025	AcMZPS	1020:1025	The results obtained in present study suggested that AcMZPS, AlMZPS and their purified fractions could be used as functional foods and natural drugs in preventing the hyperlipidemia and non-alcoholic fatty liver.
27871790	6	14	theme	purified	1045:1052	arg1	fractions					1054:1062	their purified fractions	1039:1062	their purified fractions	1039:1062	The results obtained in present study suggested that AcMZPS, AlMZPS and their purified fractions could be used as functional foods and natural drugs in preventing the hyperlipidemia and non-alcoholic fatty liver.
27871790	6	14	theme	purified	1045:1052	arg1	foods					1092:1096	functional foods	1081:1096	functional foods	1081:1096	The results obtained in present study suggested that AcMZPS, AlMZPS and their purified fractions could be used as functional foods and natural drugs in preventing the hyperlipidemia and non-alcoholic fatty liver.
27871790	6	14	theme	purified	1045:1052	arg1	drugs					1110:1114	natural drugs	1102:1114	natural drugs	1102:1114	The results obtained in present study suggested that AcMZPS, AlMZPS and their purified fractions could be used as functional foods and natural drugs in preventing the hyperlipidemia and non-alcoholic fatty liver.
27871790	0	15	theme	Antioxidant	0:10	arg1	effects					36:42	Antioxidant and anti-hyperlipidemic effects	0:42	Antioxidant and anti-hyperlipidemic effects of mycelia zinc polysaccharides by Pleurotus eryngii var	0:99	Antioxidant and anti-hyperlipidemic effects of mycelia zinc polysaccharides by Pleurotus eryngii var.
27871790	4	16	from	GSH-Px	635:640	arg1	homogenate					672:681	serum lipid/liver homogenate	654:681	serum lipid/liver homogenate	654:681	The in vivo experiments demonstrated that both AcMZPS and AlMZPS had potential hepatoprotective effects by significantly decreasing the levels of LDL-C, VLDL-C, TC, TG, ALT, AST, ALP, MDA and LPO, and remarkably increasing the HDL-C, SOD, GSH-Px, and CAT in serum lipid/liver homogenate, respectively.
27871790	4	17	dep	in	400:401	arg1	vivo					403:406	vivo	403:406	vivo	403:406	The in vivo experiments demonstrated that both AcMZPS and AlMZPS had potential hepatoprotective effects by significantly decreasing the levels of LDL-C, VLDL-C, TC, TG, ALT, AST, ALP, MDA and LPO, and remarkably increasing the HDL-C, SOD, GSH-Px, and CAT in serum lipid/liver homogenate, respectively.
27871790	0	18	theme	anti-hyperlipidemic	16:34	arg1	effects					36:42	Antioxidant and anti-hyperlipidemic effects	0:42	Antioxidant and anti-hyperlipidemic effects of mycelia zinc polysaccharides by Pleurotus eryngii var	0:99	Antioxidant and anti-hyperlipidemic effects of mycelia zinc polysaccharides by Pleurotus eryngii var.
27871790	2	19	theme	antioxidant	190:200	arg1	effects					202:208	the hepatoprotective and antioxidant effects	165:208	the hepatoprotective and antioxidant effects of acidic- and alkali-extractable mycelia zinc polysaccharides (AcMZPS, AlMZPS) from Pleurotus eryngii var	165:315	The aims of this work were designed to investigate the hepatoprotective and antioxidant effects of acidic- and alkali-extractable mycelia zinc polysaccharides (AcMZPS, AlMZPS) from Pleurotus eryngii var.
27871790	5	20	theme	in	936:937	arg1	activity					957:964	the in vitro antioxidant activity	932:964	the in vitro antioxidant activity	932:964	In addition, four polysaccharide fractions of AcMZPS-1, AcMZPS-2, AlMZPS-1, and AlMZPS-2, purified from AcMZPS and AlMZPS using DEAE chromatography, respectively, were subjected to monosaccharide composition analysis and valuated for the in vitro antioxidant activity.
27871790	4	21	from	SOD	630:632	arg1	homogenate					672:681	serum lipid/liver homogenate	654:681	serum lipid/liver homogenate	654:681	The in vivo experiments demonstrated that both AcMZPS and AlMZPS had potential hepatoprotective effects by significantly decreasing the levels of LDL-C, VLDL-C, TC, TG, ALT, AST, ALP, MDA and LPO, and remarkably increasing the HDL-C, SOD, GSH-Px, and CAT in serum lipid/liver homogenate, respectively.
27871790	6	22	theme	functional	1081:1090	arg1	AlMZPS					1028:1033	AlMZPS	1028:1033	AlMZPS	1028:1033	The results obtained in present study suggested that AcMZPS, AlMZPS and their purified fractions could be used as functional foods and natural drugs in preventing the hyperlipidemia and non-alcoholic fatty liver.
27871790	6	22	theme	functional	1081:1090	arg1	AcMZPS					1020:1025	AcMZPS	1020:1025	AcMZPS	1020:1025	The results obtained in present study suggested that AcMZPS, AlMZPS and their purified fractions could be used as functional foods and natural drugs in preventing the hyperlipidemia and non-alcoholic fatty liver.
27871790	6	22	theme	functional	1081:1090	arg1	fractions					1054:1062	their purified fractions	1039:1062	their purified fractions	1039:1062	The results obtained in present study suggested that AcMZPS, AlMZPS and their purified fractions could be used as functional foods and natural drugs in preventing the hyperlipidemia and non-alcoholic fatty liver.
27871790	6	22	theme	functional	1081:1090	arg1	foods					1092:1096	functional foods	1081:1096	functional foods	1081:1096	The results obtained in present study suggested that AcMZPS, AlMZPS and their purified fractions could be used as functional foods and natural drugs in preventing the hyperlipidemia and non-alcoholic fatty liver.
27871790	0	23	theme	mycelia	47:53	arg1	polysaccharides					60:74	mycelia zinc polysaccharides	47:74	mycelia zinc polysaccharides	47:74	Antioxidant and anti-hyperlipidemic effects of mycelia zinc polysaccharides by Pleurotus eryngii var.
27871790	6	24	theme	natural	1102:1108	arg1	AlMZPS					1028:1033	AlMZPS	1028:1033	AlMZPS	1028:1033	The results obtained in present study suggested that AcMZPS, AlMZPS and their purified fractions could be used as functional foods and natural drugs in preventing the hyperlipidemia and non-alcoholic fatty liver.
27871790	6	24	theme	natural	1102:1108	arg1	AcMZPS					1020:1025	AcMZPS	1020:1025	AcMZPS	1020:1025	The results obtained in present study suggested that AcMZPS, AlMZPS and their purified fractions could be used as functional foods and natural drugs in preventing the hyperlipidemia and non-alcoholic fatty liver.
27871790	6	24	theme	natural	1102:1108	arg1	fractions					1054:1062	their purified fractions	1039:1062	their purified fractions	1039:1062	The results obtained in present study suggested that AcMZPS, AlMZPS and their purified fractions could be used as functional foods and natural drugs in preventing the hyperlipidemia and non-alcoholic fatty liver.
27871790	6	24	theme	natural	1102:1108	arg1	drugs					1110:1114	natural drugs	1102:1114	natural drugs	1102:1114	The results obtained in present study suggested that AcMZPS, AlMZPS and their purified fractions could be used as functional foods and natural drugs in preventing the hyperlipidemia and non-alcoholic fatty liver.
27871790	2	25	theme	polysaccharides	257:271	arg1	effects					202:208	the hepatoprotective and antioxidant effects	165:208	the hepatoprotective and antioxidant effects of acidic- and alkali-extractable mycelia zinc polysaccharides (AcMZPS, AlMZPS) from Pleurotus eryngii var	165:315	The aims of this work were designed to investigate the hepatoprotective and antioxidant effects of acidic- and alkali-extractable mycelia zinc polysaccharides (AcMZPS, AlMZPS) from Pleurotus eryngii var.
27871790	4	26	contain	had	461:463	arg1	AlMZPS					454:459	AlMZPS	454:459	AlMZPS	454:459	The in vivo experiments demonstrated that both AcMZPS and AlMZPS had potential hepatoprotective effects by significantly decreasing the levels of LDL-C, VLDL-C, TC, TG, ALT, AST, ALP, MDA and LPO, and remarkably increasing the HDL-C, SOD, GSH-Px, and CAT in serum lipid/liver homogenate, respectively.
27871790	4	26	contain	had	461:463	arg1	AcMZPS					443:448	AcMZPS	443:448	AcMZPS	443:448	The in vivo experiments demonstrated that both AcMZPS and AlMZPS had potential hepatoprotective effects by significantly decreasing the levels of LDL-C, VLDL-C, TC, TG, ALT, AST, ALP, MDA and LPO, and remarkably increasing the HDL-C, SOD, GSH-Px, and CAT in serum lipid/liver homogenate, respectively.
27871790	4	26	contain	had	461:463	arg2	effects					492:498	potential hepatoprotective effects	465:498	potential hepatoprotective effects	465:498	The in vivo experiments demonstrated that both AcMZPS and AlMZPS had potential hepatoprotective effects by significantly decreasing the levels of LDL-C, VLDL-C, TC, TG, ALT, AST, ALP, MDA and LPO, and remarkably increasing the HDL-C, SOD, GSH-Px, and CAT in serum lipid/liver homogenate, respectively.
27871790	4	27	theme	potential	465:473	arg1	effects					492:498	potential hepatoprotective effects	465:498	potential hepatoprotective effects	465:498	The in vivo experiments demonstrated that both AcMZPS and AlMZPS had potential hepatoprotective effects by significantly decreasing the levels of LDL-C, VLDL-C, TC, TG, ALT, AST, ALP, MDA and LPO, and remarkably increasing the HDL-C, SOD, GSH-Px, and CAT in serum lipid/liver homogenate, respectively.
27871790	2	28	theme	hepatoprotective	169:184	arg1	effects					202:208	the hepatoprotective and antioxidant effects	165:208	the hepatoprotective and antioxidant effects of acidic- and alkali-extractable mycelia zinc polysaccharides (AcMZPS, AlMZPS) from Pleurotus eryngii var	165:315	The aims of this work were designed to investigate the hepatoprotective and antioxidant effects of acidic- and alkali-extractable mycelia zinc polysaccharides (AcMZPS, AlMZPS) from Pleurotus eryngii var.
27871790	2	29	theme	zinc	252:255	arg1	polysaccharides					257:271	acidic- and alkali-extractable mycelia zinc polysaccharides	213:271	acidic- and alkali-extractable mycelia zinc polysaccharides (AcMZPS, AlMZPS) from Pleurotus eryngii var	213:315	The aims of this work were designed to investigate the hepatoprotective and antioxidant effects of acidic- and alkali-extractable mycelia zinc polysaccharides (AcMZPS, AlMZPS) from Pleurotus eryngii var.
27871790	2	29	theme	zinc	252:255	arg1	AlMZPS					282:287	AlMZPS	282:287	AlMZPS	282:287	The aims of this work were designed to investigate the hepatoprotective and antioxidant effects of acidic- and alkali-extractable mycelia zinc polysaccharides (AcMZPS, AlMZPS) from Pleurotus eryngii var.
27871790	0	30	theme	polysaccharides	60:74	arg1	effects					36:42	Antioxidant and anti-hyperlipidemic effects	0:42	Antioxidant and anti-hyperlipidemic effects of mycelia zinc polysaccharides by Pleurotus eryngii var	0:99	Antioxidant and anti-hyperlipidemic effects of mycelia zinc polysaccharides by Pleurotus eryngii var.
27871790	2	31	theme	mycelia	244:250	arg1	polysaccharides					257:271	acidic- and alkali-extractable mycelia zinc polysaccharides	213:271	acidic- and alkali-extractable mycelia zinc polysaccharides (AcMZPS, AlMZPS) from Pleurotus eryngii var	213:315	The aims of this work were designed to investigate the hepatoprotective and antioxidant effects of acidic- and alkali-extractable mycelia zinc polysaccharides (AcMZPS, AlMZPS) from Pleurotus eryngii var.
27871790	2	31	theme	mycelia	244:250	arg1	AlMZPS					282:287	AlMZPS	282:287	AlMZPS	282:287	The aims of this work were designed to investigate the hepatoprotective and antioxidant effects of acidic- and alkali-extractable mycelia zinc polysaccharides (AcMZPS, AlMZPS) from Pleurotus eryngii var.
27871790	6	32	used	used	1073:1076	arg2	drugs					1110:1114	natural drugs	1102:1114	natural drugs	1102:1114	The results obtained in present study suggested that AcMZPS, AlMZPS and their purified fractions could be used as functional foods and natural drugs in preventing the hyperlipidemia and non-alcoholic fatty liver.
27871790	6	32	used	used	1073:1076	arg2	AcMZPS					1020:1025	AcMZPS	1020:1025	AcMZPS	1020:1025	The results obtained in present study suggested that AcMZPS, AlMZPS and their purified fractions could be used as functional foods and natural drugs in preventing the hyperlipidemia and non-alcoholic fatty liver.
27871790	6	32	used	used	1073:1076	arg2	fractions					1054:1062	their purified fractions	1039:1062	their purified fractions	1039:1062	The results obtained in present study suggested that AcMZPS, AlMZPS and their purified fractions could be used as functional foods and natural drugs in preventing the hyperlipidemia and non-alcoholic fatty liver.
27871790	6	32	used	used	1073:1076	arg2	foods					1092:1096	functional foods	1081:1096	functional foods	1081:1096	The results obtained in present study suggested that AcMZPS, AlMZPS and their purified fractions could be used as functional foods and natural drugs in preventing the hyperlipidemia and non-alcoholic fatty liver.
27871790	6	32	used	used	1073:1076	arg2	AlMZPS					1028:1033	AlMZPS	1028:1033	AlMZPS	1028:1033	The results obtained in present study suggested that AcMZPS, AlMZPS and their purified fractions could be used as functional foods and natural drugs in preventing the hyperlipidemia and non-alcoholic fatty liver.
27871790	5	33	theme	monosaccharide	879:892	arg1	analysis					906:913	monosaccharide composition analysis	879:913	monosaccharide composition analysis	879:913	In addition, four polysaccharide fractions of AcMZPS-1, AcMZPS-2, AlMZPS-1, and AlMZPS-2, purified from AcMZPS and AlMZPS using DEAE chromatography, respectively, were subjected to monosaccharide composition analysis and valuated for the in vitro antioxidant activity.
27871790	0	34	theme	zinc	55:58	arg1	polysaccharides					60:74	mycelia zinc polysaccharides	47:74	mycelia zinc polysaccharides	47:74	Antioxidant and anti-hyperlipidemic effects of mycelia zinc polysaccharides by Pleurotus eryngii var.
27871790	6	35	theme	fatty	1167:1171	arg1	liver					1173:1177	non-alcoholic fatty liver	1153:1177	non-alcoholic fatty liver	1153:1177	The results obtained in present study suggested that AcMZPS, AlMZPS and their purified fractions could be used as functional foods and natural drugs in preventing the hyperlipidemia and non-alcoholic fatty liver.
27871790	4	36	theme	hepatoprotective	475:490	arg1	effects					492:498	potential hepatoprotective effects	465:498	potential hepatoprotective effects	465:498	The in vivo experiments demonstrated that both AcMZPS and AlMZPS had potential hepatoprotective effects by significantly decreasing the levels of LDL-C, VLDL-C, TC, TG, ALT, AST, ALP, MDA and LPO, and remarkably increasing the HDL-C, SOD, GSH-Px, and CAT in serum lipid/liver homogenate, respectively.
27871790	2	37	theme	alkali-extractable	225:242	arg1	polysaccharides					257:271	acidic- and alkali-extractable mycelia zinc polysaccharides	213:271	acidic- and alkali-extractable mycelia zinc polysaccharides (AcMZPS, AlMZPS) from Pleurotus eryngii var	213:315	The aims of this work were designed to investigate the hepatoprotective and antioxidant effects of acidic- and alkali-extractable mycelia zinc polysaccharides (AcMZPS, AlMZPS) from Pleurotus eryngii var.
27871790	2	37	theme	alkali-extractable	225:242	arg1	AlMZPS					282:287	AlMZPS	282:287	AlMZPS	282:287	The aims of this work were designed to investigate the hepatoprotective and antioxidant effects of acidic- and alkali-extractable mycelia zinc polysaccharides (AcMZPS, AlMZPS) from Pleurotus eryngii var.
27871790	5	38	theme	composition	894:904	arg1	analysis					906:913	monosaccharide composition analysis	879:913	monosaccharide composition analysis	879:913	In addition, four polysaccharide fractions of AcMZPS-1, AcMZPS-2, AlMZPS-1, and AlMZPS-2, purified from AcMZPS and AlMZPS using DEAE chromatography, respectively, were subjected to monosaccharide composition analysis and valuated for the in vitro antioxidant activity.
27871790	2	39	theme	acidic-	213:219	arg1	polysaccharides					257:271	acidic- and alkali-extractable mycelia zinc polysaccharides	213:271	acidic- and alkali-extractable mycelia zinc polysaccharides (AcMZPS, AlMZPS) from Pleurotus eryngii var	213:315	The aims of this work were designed to investigate the hepatoprotective and antioxidant effects of acidic- and alkali-extractable mycelia zinc polysaccharides (AcMZPS, AlMZPS) from Pleurotus eryngii var.
27871790	2	39	theme	acidic-	213:219	arg1	AlMZPS					282:287	AlMZPS	282:287	AlMZPS	282:287	The aims of this work were designed to investigate the hepatoprotective and antioxidant effects of acidic- and alkali-extractable mycelia zinc polysaccharides (AcMZPS, AlMZPS) from Pleurotus eryngii var.
27871790	4	40	theme	in	400:401	arg1	experiments					408:418	The in vivo experiments	396:418	The in vivo experiments	396:418	The in vivo experiments demonstrated that both AcMZPS and AlMZPS had potential hepatoprotective effects by significantly decreasing the levels of LDL-C, VLDL-C, TC, TG, ALT, AST, ALP, MDA and LPO, and remarkably increasing the HDL-C, SOD, GSH-Px, and CAT in serum lipid/liver homogenate, respectively.
27871790	5	41	dep	in	936:937	arg1	vitro					939:943	vitro	939:943	vitro	939:943	In addition, four polysaccharide fractions of AcMZPS-1, AcMZPS-2, AlMZPS-1, and AlMZPS-2, purified from AcMZPS and AlMZPS using DEAE chromatography, respectively, were subjected to monosaccharide composition analysis and valuated for the in vitro antioxidant activity.
27871790	3	42	theme	hyperlipidemic	375:388	arg1	mice					390:393	hyperlipidemic mice	375:393	hyperlipidemic mice	375:393	tuoliensis on high-fat-high-cholesterol emulsion-induced hyperlipidemic mice.
27871790	4	43	theme	lipid/liver	660:670	arg1	homogenate					672:681	serum lipid/liver homogenate	654:681	serum lipid/liver homogenate	654:681	The in vivo experiments demonstrated that both AcMZPS and AlMZPS had potential hepatoprotective effects by significantly decreasing the levels of LDL-C, VLDL-C, TC, TG, ALT, AST, ALP, MDA and LPO, and remarkably increasing the HDL-C, SOD, GSH-Px, and CAT in serum lipid/liver homogenate, respectively.
27871790	3	44	from	tuoliensis	318:327	arg1	high-fat-high-cholesterol					332:356	high-fat-high-cholesterol	332:356	high-fat-high-cholesterol	332:356	tuoliensis on high-fat-high-cholesterol emulsion-induced hyperlipidemic mice.
27871790	5	45	theme	AcMZPS-1	744:751	arg1	fractions					731:739	four polysaccharide fractions	711:739	four polysaccharide fractions	711:739	In addition, four polysaccharide fractions of AcMZPS-1, AcMZPS-2, AlMZPS-1, and AlMZPS-2, purified from AcMZPS and AlMZPS using DEAE chromatography, respectively, were subjected to monosaccharide composition analysis and valuated for the in vitro antioxidant activity.
27871790	2	46	theme	work	131:134	arg1	aims					118:121	The aims	114:121	The aims of this work	114:134	The aims of this work were designed to investigate the hepatoprotective and antioxidant effects of acidic- and alkali-extractable mycelia zinc polysaccharides (AcMZPS, AlMZPS) from Pleurotus eryngii var.
27871790	5	47	theme	AlMZPS-1	764:771	arg1	fractions					731:739	four polysaccharide fractions	711:739	four polysaccharide fractions	711:739	In addition, four polysaccharide fractions of AcMZPS-1, AcMZPS-2, AlMZPS-1, and AlMZPS-2, purified from AcMZPS and AlMZPS using DEAE chromatography, respectively, were subjected to monosaccharide composition analysis and valuated for the in vitro antioxidant activity.
27871790	4	48	theme	serum	654:658	arg1	homogenate					672:681	serum lipid/liver homogenate	654:681	serum lipid/liver homogenate	654:681	The in vivo experiments demonstrated that both AcMZPS and AlMZPS had potential hepatoprotective effects by significantly decreasing the levels of LDL-C, VLDL-C, TC, TG, ALT, AST, ALP, MDA and LPO, and remarkably increasing the HDL-C, SOD, GSH-Px, and CAT in serum lipid/liver homogenate, respectively.
27871790	6	49	theme	present	991:997	arg1	study					999:1003	present study	991:1003	present study	991:1003	The results obtained in present study suggested that AcMZPS, AlMZPS and their purified fractions could be used as functional foods and natural drugs in preventing the hyperlipidemia and non-alcoholic fatty liver.
26428096	6	0	theme	water	693:697	arg1	repellency					699:708	surface water repellency	685:708	surface water repellency	685:708	Silver nanoparticles induced surface water repellency remarkably and lowered moisture permeability.
26428096	2	1	theme	bottom-up	335:343	arg1	synthesis					345:353	a biopolymer entrapped bottom-up synthesis	312:353	a biopolymer entrapped bottom-up synthesis	312:353	Assembly of molecules in lower size range (∼ 10 nm) was attained in a biopolymer entrapped bottom-up synthesis.
26428096	4	2	theme	significant	485:495	arg1	effect					497:502	any significant effect	481:502	any significant effect on particle size, distribution or plasmonic intensity	481:556	Nanoparticles encaged in cationic guar (GGAgnC) were preserved as films for months without any significant effect on particle size, distribution or plasmonic intensity.
26428096	5	3	theme	XRD	630:632	arg1	studies					647:653	FTIR, XRD, SEM and TEM studies	624:653	FTIR, XRD, SEM and TEM studies	624:653	The new nano-bio-composite and films were characterized fully in FTIR, XRD, SEM and TEM studies.
26428096	2	4	theme	entrapped	325:333	arg1	synthesis					345:353	a biopolymer entrapped bottom-up synthesis	312:353	a biopolymer entrapped bottom-up synthesis	312:353	Assembly of molecules in lower size range (∼ 10 nm) was attained in a biopolymer entrapped bottom-up synthesis.
26428096	2	5	theme	∼	287:287	arg1	nm					292:293	∼ 10 nm	287:293	∼ 10 nm	287:293	Assembly of molecules in lower size range (∼ 10 nm) was attained in a biopolymer entrapped bottom-up synthesis.
26428096	2	5	theme	∼	287:287	arg1	range					280:284	lower size range	269:284	lower size range (∼ 10 nm)	269:294	Assembly of molecules in lower size range (∼ 10 nm) was attained in a biopolymer entrapped bottom-up synthesis.
26428096	8	6	theme	microorganisms	925:938	arg1	range					916:920	a range	914:920	a range of microorganisms	914:938	GGAgnC expressed intense antimicrobial activity when tested against a range of microorganisms.
26428096	3	7	theme	filming	370:376	arg1	biopolymer					378:387	a filming biopolymer	368:387	a filming biopolymer	368:387	Guar gum is a filming biopolymer.
26428096	3	7	theme	filming	370:376	arg1	gum					361:363	Guar gum	356:363	Guar gum	356:363	Guar gum is a filming biopolymer.
26428096	9	8	from	nanoparticles	960:972	arg1	GGAA					977:980	GGAA	977:980	GGAA	977:980	Immobilized silver nanoparticles in GGAA can feasibly be used as filming microbicidals suitable for textiles, packaging and biomedical device applications.
26428096	5	9	theme	SEM	635:637	arg1	studies					647:653	FTIR, XRD, SEM and TEM studies	624:653	FTIR, XRD, SEM and TEM studies	624:653	The new nano-bio-composite and films were characterized fully in FTIR, XRD, SEM and TEM studies.
26428096	6	10	theme	surface	685:691	arg1	repellency					699:708	surface water repellency	685:708	surface water repellency	685:708	Silver nanoparticles induced surface water repellency remarkably and lowered moisture permeability.
26428096	9	11	theme	device	1076:1081	arg1	applications					1083:1094	biomedical device applications	1065:1094	biomedical device applications	1065:1094	Immobilized silver nanoparticles in GGAA can feasibly be used as filming microbicidals suitable for textiles, packaging and biomedical device applications.
26428096	2	12	theme	biopolymer	314:323	arg1	synthesis					345:353	a biopolymer entrapped bottom-up synthesis	312:353	a biopolymer entrapped bottom-up synthesis	312:353	Assembly of molecules in lower size range (∼ 10 nm) was attained in a biopolymer entrapped bottom-up synthesis.
26428096	1	13	from	evaluation	165:174	arg1	GGAA					237:240	GGAA	237:240	GGAA	237:240	This work is meant for environmentally friendly synthesis and functional evaluation of silver nanoparticles in a newer cationic guar biopolymer (GGAA).
26428096	1	13	from	evaluation	165:174	arg1	biopolymer					225:234	a newer cationic guar biopolymer	203:234	a newer cationic guar biopolymer (GGAA)	203:241	This work is meant for environmentally friendly synthesis and functional evaluation of silver nanoparticles in a newer cationic guar biopolymer (GGAA).
26428096	1	14	theme	nanoparticles	186:198	arg1	synthesis					140:148	environmentally friendly synthesis	115:148	environmentally friendly synthesis	115:148	This work is meant for environmentally friendly synthesis and functional evaluation of silver nanoparticles in a newer cationic guar biopolymer (GGAA).
26428096	1	14	theme	nanoparticles	186:198	arg1	evaluation					165:174	functional evaluation	154:174	functional evaluation of silver nanoparticles in a newer cationic guar biopolymer (GGAA)	154:241	This work is meant for environmentally friendly synthesis and functional evaluation of silver nanoparticles in a newer cationic guar biopolymer (GGAA).
26428096	1	15	from	synthesis	140:148	arg1	GGAA					237:240	GGAA	237:240	GGAA	237:240	This work is meant for environmentally friendly synthesis and functional evaluation of silver nanoparticles in a newer cationic guar biopolymer (GGAA).
26428096	1	15	from	synthesis	140:148	arg1	biopolymer					225:234	a newer cationic guar biopolymer	203:234	a newer cationic guar biopolymer (GGAA)	203:241	This work is meant for environmentally friendly synthesis and functional evaluation of silver nanoparticles in a newer cationic guar biopolymer (GGAA).
26428096	2	16	theme	size	275:278	arg1	nm					292:293	∼ 10 nm	287:293	∼ 10 nm	287:293	Assembly of molecules in lower size range (∼ 10 nm) was attained in a biopolymer entrapped bottom-up synthesis.
26428096	2	16	theme	size	275:278	arg1	range					280:284	lower size range	269:284	lower size range (∼ 10 nm)	269:294	Assembly of molecules in lower size range (∼ 10 nm) was attained in a biopolymer entrapped bottom-up synthesis.
26428096	2	17	from	Assembly	244:251	arg1	nm					292:293	∼ 10 nm	287:293	∼ 10 nm	287:293	Assembly of molecules in lower size range (∼ 10 nm) was attained in a biopolymer entrapped bottom-up synthesis.
26428096	2	17	from	Assembly	244:251	arg1	range					280:284	lower size range	269:284	lower size range (∼ 10 nm)	269:294	Assembly of molecules in lower size range (∼ 10 nm) was attained in a biopolymer entrapped bottom-up synthesis.
26428096	0	18	theme	guar	9:12	arg1	gum					14:16	Cationic guar gum	0:16	Cationic guar gum	0:16	Cationic guar gum orchestrated environmental synthesis for silver nano-bio-composite films.
26428096	6	19	theme	Silver	656:661	arg1	nanoparticles					663:675	Silver nanoparticles	656:675	Silver nanoparticles	656:675	Silver nanoparticles induced surface water repellency remarkably and lowered moisture permeability.
26428096	0	20	theme	Cationic	0:7	arg1	gum					14:16	Cationic guar gum	0:16	Cationic guar gum	0:16	Cationic guar gum orchestrated environmental synthesis for silver nano-bio-composite films.
26428096	5	21	theme	FTIR	624:627	arg1	studies					647:653	FTIR, XRD, SEM and TEM studies	624:653	FTIR, XRD, SEM and TEM studies	624:653	The new nano-bio-composite and films were characterized fully in FTIR, XRD, SEM and TEM studies.
26428096	2	22	from	molecules	256:264	arg1	nm					292:293	∼ 10 nm	287:293	∼ 10 nm	287:293	Assembly of molecules in lower size range (∼ 10 nm) was attained in a biopolymer entrapped bottom-up synthesis.
26428096	2	22	from	molecules	256:264	arg1	range					280:284	lower size range	269:284	lower size range (∼ 10 nm)	269:294	Assembly of molecules in lower size range (∼ 10 nm) was attained in a biopolymer entrapped bottom-up synthesis.
26428096	1	23	theme	newer	205:209	arg1	GGAA					237:240	GGAA	237:240	GGAA	237:240	This work is meant for environmentally friendly synthesis and functional evaluation of silver nanoparticles in a newer cationic guar biopolymer (GGAA).
26428096	1	23	theme	newer	205:209	arg1	biopolymer					225:234	a newer cationic guar biopolymer	203:234	a newer cationic guar biopolymer (GGAA)	203:241	This work is meant for environmentally friendly synthesis and functional evaluation of silver nanoparticles in a newer cationic guar biopolymer (GGAA).
26428096	3	24	theme	Guar	356:359	arg1	biopolymer					378:387	a filming biopolymer	368:387	a filming biopolymer	368:387	Guar gum is a filming biopolymer.
26428096	3	24	theme	Guar	356:359	arg1	gum					361:363	Guar gum	356:363	Guar gum	356:363	Guar gum is a filming biopolymer.
26428096	5	25	theme	new	563:565	arg1	nano-bio-composite					567:584	The new nano-bio-composite	559:584	The new nano-bio-composite	559:584	The new nano-bio-composite and films were characterized fully in FTIR, XRD, SEM and TEM studies.
26428096	1	26	theme	cationic	211:218	arg1	GGAA					237:240	GGAA	237:240	GGAA	237:240	This work is meant for environmentally friendly synthesis and functional evaluation of silver nanoparticles in a newer cationic guar biopolymer (GGAA).
26428096	1	26	theme	cationic	211:218	arg1	biopolymer					225:234	a newer cationic guar biopolymer	203:234	a newer cationic guar biopolymer (GGAA)	203:241	This work is meant for environmentally friendly synthesis and functional evaluation of silver nanoparticles in a newer cationic guar biopolymer (GGAA).
26428096	4	27	theme	plasmonic	538:546	arg1	intensity					548:556	plasmonic intensity	538:556	plasmonic intensity	538:556	Nanoparticles encaged in cationic guar (GGAgnC) were preserved as films for months without any significant effect on particle size, distribution or plasmonic intensity.
26428096	1	28	theme	silver	179:184	arg1	nanoparticles					186:198	silver nanoparticles	179:198	silver nanoparticles	179:198	This work is meant for environmentally friendly synthesis and functional evaluation of silver nanoparticles in a newer cationic guar biopolymer (GGAA).
26428096	1	29	theme	guar	220:223	arg1	GGAA					237:240	GGAA	237:240	GGAA	237:240	This work is meant for environmentally friendly synthesis and functional evaluation of silver nanoparticles in a newer cationic guar biopolymer (GGAA).
26428096	1	29	theme	guar	220:223	arg1	biopolymer					225:234	a newer cationic guar biopolymer	203:234	a newer cationic guar biopolymer (GGAA)	203:241	This work is meant for environmentally friendly synthesis and functional evaluation of silver nanoparticles in a newer cationic guar biopolymer (GGAA).
26428096	8	30	theme	antimicrobial	871:883	arg1	activity					885:892	intense antimicrobial activity	863:892	intense antimicrobial activity	863:892	GGAgnC expressed intense antimicrobial activity when tested against a range of microorganisms.
26428096	0	31	theme	environmental	31:43	arg1	synthesis					45:53	environmental synthesis	31:53	environmental synthesis	31:53	Cationic guar gum orchestrated environmental synthesis for silver nano-bio-composite films.
26428096	7	32	theme	water	768:772	arg1	115°					804:807	115°	804:807	115°	804:807	GGAgnC film water contact angle was recorded as 115° while, that in case of GGAA was 59°.
26428096	7	32	theme	water	768:772	arg1	angle					782:786	GGAgnC film water contact angle	756:786	GGAgnC film water contact angle	756:786	GGAgnC film water contact angle was recorded as 115° while, that in case of GGAA was 59°.
26428096	7	33	theme	film	763:766	arg1	115°					804:807	115°	804:807	115°	804:807	GGAgnC film water contact angle was recorded as 115° while, that in case of GGAA was 59°.
26428096	7	33	theme	film	763:766	arg1	angle					782:786	GGAgnC film water contact angle	756:786	GGAgnC film water contact angle	756:786	GGAgnC film water contact angle was recorded as 115° while, that in case of GGAA was 59°.
26428096	0	34	theme	silver	59:64	arg1	films					85:89	silver nano-bio-composite films	59:89	silver nano-bio-composite films	59:89	Cationic guar gum orchestrated environmental synthesis for silver nano-bio-composite films.
26428096	7	35	theme	contact	774:780	arg1	115°					804:807	115°	804:807	115°	804:807	GGAgnC film water contact angle was recorded as 115° while, that in case of GGAA was 59°.
26428096	7	35	theme	contact	774:780	arg1	angle					782:786	GGAgnC film water contact angle	756:786	GGAgnC film water contact angle	756:786	GGAgnC film water contact angle was recorded as 115° while, that in case of GGAA was 59°.
26428096	1	36	theme	environmentally	115:129	arg1	synthesis					140:148	environmentally friendly synthesis	115:148	environmentally friendly synthesis	115:148	This work is meant for environmentally friendly synthesis and functional evaluation of silver nanoparticles in a newer cationic guar biopolymer (GGAA).
26428096	4	37	from	effect	497:502	arg1	distribution					522:533	distribution	522:533	distribution	522:533	Nanoparticles encaged in cationic guar (GGAgnC) were preserved as films for months without any significant effect on particle size, distribution or plasmonic intensity.
26428096	4	37	from	effect	497:502	arg1	intensity					548:556	plasmonic intensity	538:556	plasmonic intensity	538:556	Nanoparticles encaged in cationic guar (GGAgnC) were preserved as films for months without any significant effect on particle size, distribution or plasmonic intensity.
26428096	4	37	from	effect	497:502	arg1	size					516:519	particle size	507:519	particle size	507:519	Nanoparticles encaged in cationic guar (GGAgnC) were preserved as films for months without any significant effect on particle size, distribution or plasmonic intensity.
26428096	7	38	theme	GGAgnC	756:761	arg1	115°					804:807	115°	804:807	115°	804:807	GGAgnC film water contact angle was recorded as 115° while, that in case of GGAA was 59°.
26428096	7	38	theme	GGAgnC	756:761	arg1	angle					782:786	GGAgnC film water contact angle	756:786	GGAgnC film water contact angle	756:786	GGAgnC film water contact angle was recorded as 115° while, that in case of GGAA was 59°.
26428096	5	39	theme	TEM	643:645	arg1	studies					647:653	FTIR, XRD, SEM and TEM studies	624:653	FTIR, XRD, SEM and TEM studies	624:653	The new nano-bio-composite and films were characterized fully in FTIR, XRD, SEM and TEM studies.
26428096	8	40	theme	intense	863:869	arg1	activity					885:892	intense antimicrobial activity	863:892	intense antimicrobial activity	863:892	GGAgnC expressed intense antimicrobial activity when tested against a range of microorganisms.
26428096	6	41	theme	moisture	733:740	arg1	permeability					742:753	moisture permeability	733:753	moisture permeability	733:753	Silver nanoparticles induced surface water repellency remarkably and lowered moisture permeability.
26428096	1	42	theme	friendly	131:138	arg1	synthesis					140:148	environmentally friendly synthesis	115:148	environmentally friendly synthesis	115:148	This work is meant for environmentally friendly synthesis and functional evaluation of silver nanoparticles in a newer cationic guar biopolymer (GGAA).
26428096	2	43	from	range	280:284	arg1	Assembly					244:251	Assembly	244:251	Assembly of molecules in lower size range (∼ 10 nm)	244:294	Assembly of molecules in lower size range (∼ 10 nm) was attained in a biopolymer entrapped bottom-up synthesis.
26428096	4	44	theme	particle	507:514	arg1	size					516:519	particle size	507:519	particle size	507:519	Nanoparticles encaged in cationic guar (GGAgnC) were preserved as films for months without any significant effect on particle size, distribution or plasmonic intensity.
26428096	2	45	theme	lower	269:273	arg1	nm					292:293	∼ 10 nm	287:293	∼ 10 nm	287:293	Assembly of molecules in lower size range (∼ 10 nm) was attained in a biopolymer entrapped bottom-up synthesis.
26428096	2	45	theme	lower	269:273	arg1	range					280:284	lower size range	269:284	lower size range (∼ 10 nm)	269:294	Assembly of molecules in lower size range (∼ 10 nm) was attained in a biopolymer entrapped bottom-up synthesis.
26428096	9	46	used	used	998:1001	arg2	microbicidals					1014:1026	filming microbicidals	1006:1026	filming microbicidals suitable for textiles, packaging and biomedical device applications	1006:1094	Immobilized silver nanoparticles in GGAA can feasibly be used as filming microbicidals suitable for textiles, packaging and biomedical device applications.
26428096	9	46	used	used	998:1001	arg2	nanoparticles					960:972	Immobilized silver nanoparticles	941:972	Immobilized silver nanoparticles in GGAA	941:980	Immobilized silver nanoparticles in GGAA can feasibly be used as filming microbicidals suitable for textiles, packaging and biomedical device applications.
26428096	9	47	theme	filming	1006:1012	arg1	nanoparticles					960:972	Immobilized silver nanoparticles	941:972	Immobilized silver nanoparticles in GGAA	941:980	Immobilized silver nanoparticles in GGAA can feasibly be used as filming microbicidals suitable for textiles, packaging and biomedical device applications.
26428096	9	47	theme	filming	1006:1012	arg1	microbicidals					1014:1026	filming microbicidals	1006:1026	filming microbicidals suitable for textiles, packaging and biomedical device applications	1006:1094	Immobilized silver nanoparticles in GGAA can feasibly be used as filming microbicidals suitable for textiles, packaging and biomedical device applications.
26428096	9	48	theme	biomedical	1065:1074	arg1	applications					1083:1094	biomedical device applications	1065:1094	biomedical device applications	1065:1094	Immobilized silver nanoparticles in GGAA can feasibly be used as filming microbicidals suitable for textiles, packaging and biomedical device applications.
26428096	4	49	theme	cationic	415:422	arg1	GGAgnC					430:435	GGAgnC	430:435	GGAgnC	430:435	Nanoparticles encaged in cationic guar (GGAgnC) were preserved as films for months without any significant effect on particle size, distribution or plasmonic intensity.
26428096	4	49	theme	cationic	415:422	arg1	guar					424:427	cationic guar	415:427	cationic guar (GGAgnC)	415:436	Nanoparticles encaged in cationic guar (GGAgnC) were preserved as films for months without any significant effect on particle size, distribution or plasmonic intensity.
26428096	2	50	theme	molecules	256:264	arg1	Assembly					244:251	Assembly	244:251	Assembly of molecules in lower size range (∼ 10 nm)	244:294	Assembly of molecules in lower size range (∼ 10 nm) was attained in a biopolymer entrapped bottom-up synthesis.
26428096	9	51	theme	silver	953:958	arg1	nanoparticles					960:972	Immobilized silver nanoparticles	941:972	Immobilized silver nanoparticles in GGAA	941:980	Immobilized silver nanoparticles in GGAA can feasibly be used as filming microbicidals suitable for textiles, packaging and biomedical device applications.
26428096	9	51	theme	silver	953:958	arg1	microbicidals					1014:1026	filming microbicidals	1006:1026	filming microbicidals suitable for textiles, packaging and biomedical device applications	1006:1094	Immobilized silver nanoparticles in GGAA can feasibly be used as filming microbicidals suitable for textiles, packaging and biomedical device applications.
26428096	1	52	theme	functional	154:163	arg1	evaluation					165:174	functional evaluation	154:174	functional evaluation of silver nanoparticles in a newer cationic guar biopolymer (GGAA)	154:241	This work is meant for environmentally friendly synthesis and functional evaluation of silver nanoparticles in a newer cationic guar biopolymer (GGAA).
26428096	0	53	theme	nano-bio-composite	66:83	arg1	films					85:89	silver nano-bio-composite films	59:89	silver nano-bio-composite films	59:89	Cationic guar gum orchestrated environmental synthesis for silver nano-bio-composite films.
26428096	9	54	theme	Immobilized	941:951	arg1	nanoparticles					960:972	Immobilized silver nanoparticles	941:972	Immobilized silver nanoparticles in GGAA	941:980	Immobilized silver nanoparticles in GGAA can feasibly be used as filming microbicidals suitable for textiles, packaging and biomedical device applications.
26428096	9	54	theme	Immobilized	941:951	arg1	microbicidals					1014:1026	filming microbicidals	1006:1026	filming microbicidals suitable for textiles, packaging and biomedical device applications	1006:1094	Immobilized silver nanoparticles in GGAA can feasibly be used as filming microbicidals suitable for textiles, packaging and biomedical device applications.
26428096	9	55	theme	suitable	1028:1035	arg1	nanoparticles					960:972	Immobilized silver nanoparticles	941:972	Immobilized silver nanoparticles in GGAA	941:980	Immobilized silver nanoparticles in GGAA can feasibly be used as filming microbicidals suitable for textiles, packaging and biomedical device applications.
26428096	9	55	theme	suitable	1028:1035	arg1	microbicidals					1014:1026	filming microbicidals	1006:1026	filming microbicidals suitable for textiles, packaging and biomedical device applications	1006:1094	Immobilized silver nanoparticles in GGAA can feasibly be used as filming microbicidals suitable for textiles, packaging and biomedical device applications.
29319513	3	0	theme	EPS	555:557	arg1	production					541:550	the production	537:550	the production of EPS by marine Bacillus subtilis SH1 in addition to characterization and investigation of different valuable applications	537:674	The present study attempt to optimize the production of EPS by marine Bacillus subtilis SH1 in addition to characterization and investigation of different valuable applications.
29319513	14	1	theme	sample	2152:2157	arg1	concentration					2131:2143	the concentration	2127:2143	the concentration of the sample	2127:2157	The antioxidant capacity increased with increasing the concentration of the sample.
29319513	2	2	theme	Many	302:305	arg1	microorganisms					307:320	Many microorganisms	302:320	Many microorganisms	302:320	Many microorganisms possess the ability to synthesize and excrete exopolysaccharides with novel chemical compositions, properties and structures to have potential applications in different fields.
29319513	12	3	theme	cell	1977:1980	arg1	HepG2					2008:2012	HepG2	2008:2012	HepG2	2008:2012	The EPS exhibited various degree of antitumor effect toward the tested cell lines (MCF-7, HCT-116 and HepG2).
29319513	12	3	theme	cell	1977:1980	arg1	HCT-116					1996:2002	HCT-116	1996:2002	HCT-116	1996:2002	The EPS exhibited various degree of antitumor effect toward the tested cell lines (MCF-7, HCT-116 and HepG2).
29319513	12	3	theme	cell	1977:1980	arg1	MCF-7					1989:1993	MCF-7	1989:1993	MCF-7	1989:1993	The EPS exhibited various degree of antitumor effect toward the tested cell lines (MCF-7, HCT-116 and HepG2).
29319513	12	3	theme	cell	1977:1980	arg1	lines					1982:1986	the tested cell lines	1966:1986	the tested cell lines (MCF-7, HCT-116 and HepG2)	1966:2013	The EPS exhibited various degree of antitumor effect toward the tested cell lines (MCF-7, HCT-116 and HepG2).
29319513	3	4	dep	Bacillus	569:576	arg1	subtilis					578:585	subtilis	578:585	subtilis	578:585	The present study attempt to optimize the production of EPS by marine Bacillus subtilis SH1 in addition to characterization and investigation of different valuable applications.
29319513	15	5	contain	had	2215:2217	arg1	EPS					2211:2213	EPS	2211:2213	EPS	2211:2213	Scanning electron microscopic analysis showed that EPS had compact film-like structure, which could make it a useful in the future applications as in preparing plasticized film.
29319513	15	5	contain	had	2215:2217	arg2	structure					2237:2245	compact film-like structure	2219:2245	compact film-like structure	2219:2245	Scanning electron microscopic analysis showed that EPS had compact film-like structure, which could make it a useful in the future applications as in preparing plasticized film.
29319513	1	6	theme	different	187:195	arg1	residues					203:210	different sugar residues	187:210	different sugar residues	187:210	Exopolysaccharides (EPSs) are high molecular weight polymers consisting of different sugar residues they are preferable for replacing synthetic polymers as they are degradable and nontoxic.
29319513	12	7	theme	effect	1952:1957	arg1	degree					1932:1937	various degree	1924:1937	various degree of antitumor effect	1924:1957	The EPS exhibited various degree of antitumor effect toward the tested cell lines (MCF-7, HCT-116 and HepG2).
29319513	4	8	theme	type	694:697	arg1	Effect					677:682	Effect	677:682	Effect of medium type, incubation period and pH	677:723	Effect of medium type, incubation period and pH were studied using the one factor at a time experiments.
29319513	11	9	theme	highest	1788:1794	arg1	activity					1810:1817	the highest antibacterial activity	1784:1817	the highest antibacterial activity (7.8, 9 and 10.4 AU/ml)	1784:1841	Results concluded that, EPS exhibited antibacterial activity against Aeromonas hydrophila, Pseudomonas aeruginosa and Streptococcus faecalis and the highest antibacterial activity (7.8, 9 and 10.4 AU/ml) was against S. faecalis at 50, 100 and 200 mg/ml respectively.
29319513	11	9	theme	highest	1788:1794	arg1	AU/ml					1836:1840	7.8, 9 and 10.4 AU/ml	1820:1840	7.8, 9 and 10.4 AU/ml	1820:1840	Results concluded that, EPS exhibited antibacterial activity against Aeromonas hydrophila, Pseudomonas aeruginosa and Streptococcus faecalis and the highest antibacterial activity (7.8, 9 and 10.4 AU/ml) was against S. faecalis at 50, 100 and 200 mg/ml respectively.
29319513	9	10	theme	HPLC	1415:1418	arg1	chromatogram					1420:1431	HPLC chromatogram	1415:1431	HPLC chromatogram	1415:1431	Moreover, HPLC chromatogram indicated that the EPS was a heteropolysaccharide consisting of maltose and rhamnose.
29319513	6	11	theme	Response	974:981	arg1	RSM					1004:1006	RSM	1004:1006	RSM	1004:1006	Experimental design using Response Surface Methodology (RSM) was applied to optimize various nutrients at different concentrations.
29319513	6	11	theme	Response	974:981	arg1	Methodology					991:1001	Response Surface Methodology	974:1001	Response Surface Methodology (RSM)	974:1007	Experimental design using Response Surface Methodology (RSM) was applied to optimize various nutrients at different concentrations.
29319513	8	12	theme	various	1388:1394	arg1	regions					1396:1402	various regions	1388:1402	various regions	1388:1402	Chemical characterization of the extracted EPS showed that, FTIR spectra exhibited bands at various regions.
29319513	4	13	theme	incubation	700:709	arg1	period					711:716	incubation period	700:716	incubation period	700:716	Effect of medium type, incubation period and pH were studied using the one factor at a time experiments.
29319513	10	14	theme	extracted	1578:1586	arg1	polysaccharides					1588:1602	the extracted polysaccharides	1574:1602	the extracted polysaccharides	1574:1602	The study was extended to evaluate the potentiality of the extracted polysaccharides in different medical applications.
29319513	7	15	theme	finalized	1084:1092	arg1	medium					1104:1109	The finalized optimized medium	1080:1109	The finalized optimized medium contained (gl-1) glucose (5), peptone (2.5), yeast extract (4.5) and malt extract (4.5)	1080:1197	The finalized optimized medium contained (gl-1) glucose (5), peptone (2.5), yeast extract (4.5) and malt extract (4.5) increased the production of EPS to 33.8 gl-1 with1.4 fold increase compared to the basal medium.
29319513	3	16	theme	valuable	654:661	arg1	applications					663:674	different valuable applications	644:674	different valuable applications	644:674	The present study attempt to optimize the production of EPS by marine Bacillus subtilis SH1 in addition to characterization and investigation of different valuable applications.
29319513	5	17	theme	yeast	879:883	arg1	medium					898:903	yeast malt glucose medium	879:903	yeast malt glucose medium with pH 9 at the fourth day of incubation	879:945	It was shown that the highest productivity (24 gl-1) of exopolysaccharides was recorded by using yeast malt glucose medium with pH 9 at the fourth day of incubation.
29319513	12	18	theme	various	1924:1930	arg1	degree					1932:1937	various degree	1924:1937	various degree of antitumor effect	1924:1957	The EPS exhibited various degree of antitumor effect toward the tested cell lines (MCF-7, HCT-116 and HepG2).
29319513	13	19	theme	antiviral	2043:2051	arg1	activity					2053:2060	antiviral activity	2043:2060	antiviral activity	2043:2060	In addition, EPS exhibited antiviral activity at 500 μg/ml.
29319513	5	20	theme	incubation	936:945	arg1	day					929:931	the fourth day	918:931	the fourth day of incubation	918:945	It was shown that the highest productivity (24 gl-1) of exopolysaccharides was recorded by using yeast malt glucose medium with pH 9 at the fourth day of incubation.
29319513	6	21	theme	various	1033:1039	arg1	nutrients					1041:1049	various nutrients	1033:1049	various nutrients	1033:1049	Experimental design using Response Surface Methodology (RSM) was applied to optimize various nutrients at different concentrations.
29319513	1	22	theme	weight	157:162	arg1	Exopolysaccharides					112:129	Exopolysaccharides	112:129	Exopolysaccharides (EPSs)	112:136	Exopolysaccharides (EPSs) are high molecular weight polymers consisting of different sugar residues they are preferable for replacing synthetic polymers as they are degradable and nontoxic.
29319513	1	22	theme	weight	157:162	arg1	polymers					164:171	high molecular weight polymers	142:171	high molecular weight polymers consisting of different sugar residues	142:210	Exopolysaccharides (EPSs) are high molecular weight polymers consisting of different sugar residues they are preferable for replacing synthetic polymers as they are degradable and nontoxic.
29319513	0	23	from	Characterization	12:27	arg1	Marine					82:87	Marine	82:87	Marine	82:87	Production, Characterization and Valuable Applications of Exopolysaccharides from Marine Bacillus subtilis SH1.
29319513	0	24	theme	Bacillus	89:96	arg1	SH1					107:109	Bacillus subtilis SH1	89:109	Bacillus subtilis SH1	89:109	Production, Characterization and Valuable Applications of Exopolysaccharides from Marine Bacillus subtilis SH1.
29319513	15	25	theme	future	2284:2289	arg1	applications					2291:2302	the future applications	2280:2302	the future applications as in preparing plasticized film	2280:2335	Scanning electron microscopic analysis showed that EPS had compact film-like structure, which could make it a useful in the future applications as in preparing plasticized film.
29319513	0	26	from	Production	0:9	arg1	Marine					82:87	Marine	82:87	Marine	82:87	Production, Characterization and Valuable Applications of Exopolysaccharides from Marine Bacillus subtilis SH1.
29319513	1	27	theme	high	142:145	arg1	Exopolysaccharides					112:129	Exopolysaccharides	112:129	Exopolysaccharides (EPSs)	112:136	Exopolysaccharides (EPSs) are high molecular weight polymers consisting of different sugar residues they are preferable for replacing synthetic polymers as they are degradable and nontoxic.
29319513	1	27	theme	high	142:145	arg1	polymers					164:171	high molecular weight polymers	142:171	high molecular weight polymers consisting of different sugar residues	142:210	Exopolysaccharides (EPSs) are high molecular weight polymers consisting of different sugar residues they are preferable for replacing synthetic polymers as they are degradable and nontoxic.
29319513	0	28	dep	SH1	107:109	arg1	Characterization					12:27	Characterization	12:27	Characterization	12:27	Production, Characterization and Valuable Applications of Exopolysaccharides from Marine Bacillus subtilis SH1.
29319513	0	28	dep	SH1	107:109	arg1	Applications					42:53	Valuable Applications	33:53	Valuable Applications	33:53	Production, Characterization and Valuable Applications of Exopolysaccharides from Marine Bacillus subtilis SH1.
29319513	0	28	dep	SH1	107:109	arg1	Production					0:9	Production	0:9	Production	0:9	Production, Characterization and Valuable Applications of Exopolysaccharides from Marine Bacillus subtilis SH1.
29319513	7	29	theme	basal	1282:1286	arg1	medium					1288:1293	the basal medium	1278:1293	the basal medium	1278:1293	The finalized optimized medium contained (gl-1) glucose (5), peptone (2.5), yeast extract (4.5) and malt extract (4.5) increased the production of EPS to 33.8 gl-1 with1.4 fold increase compared to the basal medium.
29319513	10	30	theme	different	1607:1615	arg1	applications					1625:1636	different medical applications	1607:1636	different medical applications	1607:1636	The study was extended to evaluate the potentiality of the extracted polysaccharides in different medical applications.
29319513	0	31	dep	Bacillus	89:96	arg1	subtilis					98:105	subtilis	98:105	subtilis	98:105	Production, Characterization and Valuable Applications of Exopolysaccharides from Marine Bacillus subtilis SH1.
29319513	11	32	theme	antibacterial	1677:1689	arg1	activity					1691:1698	antibacterial activity	1677:1698	antibacterial activity against Aeromonas hydrophila, Pseudomonas aeruginosa and Streptococcus faecalis	1677:1778	Results concluded that, EPS exhibited antibacterial activity against Aeromonas hydrophila, Pseudomonas aeruginosa and Streptococcus faecalis and the highest antibacterial activity (7.8, 9 and 10.4 AU/ml) was against S. faecalis at 50, 100 and 200 mg/ml respectively.
29319513	2	33	theme	different	481:489	arg1	fields					491:496	different fields	481:496	different fields	481:496	Many microorganisms possess the ability to synthesize and excrete exopolysaccharides with novel chemical compositions, properties and structures to have potential applications in different fields.
29319513	8	34	theme	EPS	1339:1341	arg1	characterization					1305:1320	Chemical characterization	1296:1320	Chemical characterization of the extracted EPS	1296:1341	Chemical characterization of the extracted EPS showed that, FTIR spectra exhibited bands at various regions.
29319513	3	35	theme	study	511:515	arg1	attempt					517:523	The present study attempt	499:523	The present study attempt	499:523	The present study attempt to optimize the production of EPS by marine Bacillus subtilis SH1 in addition to characterization and investigation of different valuable applications.
29319513	15	36	theme	compact	2219:2225	arg1	structure					2237:2245	compact film-like structure	2219:2245	compact film-like structure	2219:2245	Scanning electron microscopic analysis showed that EPS had compact film-like structure, which could make it a useful in the future applications as in preparing plasticized film.
29319513	6	37	theme	different	1054:1062	arg1	concentrations					1064:1077	different concentrations	1054:1077	different concentrations	1054:1077	Experimental design using Response Surface Methodology (RSM) was applied to optimize various nutrients at different concentrations.
29319513	0	38	theme	Valuable	33:40	arg1	Applications					42:53	Valuable Applications	33:53	Valuable Applications	33:53	Production, Characterization and Valuable Applications of Exopolysaccharides from Marine Bacillus subtilis SH1.
29319513	2	39	contain	possess	322:328	arg1	microorganisms					307:320	Many microorganisms	302:320	Many microorganisms	302:320	Many microorganisms possess the ability to synthesize and excrete exopolysaccharides with novel chemical compositions, properties and structures to have potential applications in different fields.
29319513	2	39	contain	possess	322:328	arg2	ability					334:340	the ability to synthesize and excrete exopolysaccharides with novel chemical compositions, properties and structures to have potential applications in different fields	330:496	the ability to synthesize and excrete exopolysaccharides with novel chemical compositions, properties and structures to have potential applications in different fields	330:496	Many microorganisms possess the ability to synthesize and excrete exopolysaccharides with novel chemical compositions, properties and structures to have potential applications in different fields.
29319513	2	40	with	exopolysaccharides	368:385	arg1	structures					436:445	structures	436:445	structures	436:445	Many microorganisms possess the ability to synthesize and excrete exopolysaccharides with novel chemical compositions, properties and structures to have potential applications in different fields.
29319513	2	40	with	exopolysaccharides	368:385	arg1	properties					421:430	properties	421:430	properties	421:430	Many microorganisms possess the ability to synthesize and excrete exopolysaccharides with novel chemical compositions, properties and structures to have potential applications in different fields.
29319513	2	40	with	exopolysaccharides	368:385	arg1	compositions					407:418	novel chemical compositions	392:418	novel chemical compositions	392:418	Many microorganisms possess the ability to synthesize and excrete exopolysaccharides with novel chemical compositions, properties and structures to have potential applications in different fields.
29319513	5	41	with	medium	898:903	arg1	pH					910:911	pH 9	910:913	pH 9	910:913	It was shown that the highest productivity (24 gl-1) of exopolysaccharides was recorded by using yeast malt glucose medium with pH 9 at the fourth day of incubation.
29319513	8	42	theme	FTIR	1356:1359	arg1	spectra					1361:1367	FTIR spectra	1356:1367	FTIR spectra	1356:1367	Chemical characterization of the extracted EPS showed that, FTIR spectra exhibited bands at various regions.
29319513	7	43	theme	EPS	1227:1229	arg1	production					1213:1222	the production	1209:1222	the production of EPS	1209:1229	The finalized optimized medium contained (gl-1) glucose (5), peptone (2.5), yeast extract (4.5) and malt extract (4.5) increased the production of EPS to 33.8 gl-1 with1.4 fold increase compared to the basal medium.
29319513	5	44	theme	glucose	890:896	arg1	medium					898:903	yeast malt glucose medium	879:903	yeast malt glucose medium with pH 9 at the fourth day of incubation	879:945	It was shown that the highest productivity (24 gl-1) of exopolysaccharides was recorded by using yeast malt glucose medium with pH 9 at the fourth day of incubation.
29319513	2	45	theme	novel	392:396	arg1	compositions					407:418	novel chemical compositions	392:418	novel chemical compositions	392:418	Many microorganisms possess the ability to synthesize and excrete exopolysaccharides with novel chemical compositions, properties and structures to have potential applications in different fields.
29319513	10	46	from	potentiality	1558:1569	arg1	applications					1625:1636	different medical applications	1607:1636	different medical applications	1607:1636	The study was extended to evaluate the potentiality of the extracted polysaccharides in different medical applications.
29319513	3	47	theme	Bacillus	569:576	arg1	SH1					587:589	marine Bacillus subtilis SH1	562:589	marine Bacillus subtilis SH1 in addition to characterization and investigation of different valuable applications	562:674	The present study attempt to optimize the production of EPS by marine Bacillus subtilis SH1 in addition to characterization and investigation of different valuable applications.
29319513	5	48	theme	malt	885:888	arg1	medium					898:903	yeast malt glucose medium	879:903	yeast malt glucose medium with pH 9 at the fourth day of incubation	879:945	It was shown that the highest productivity (24 gl-1) of exopolysaccharides was recorded by using yeast malt glucose medium with pH 9 at the fourth day of incubation.
29319513	12	49	theme	tested	1970:1975	arg1	HepG2					2008:2012	HepG2	2008:2012	HepG2	2008:2012	The EPS exhibited various degree of antitumor effect toward the tested cell lines (MCF-7, HCT-116 and HepG2).
29319513	12	49	theme	tested	1970:1975	arg1	HCT-116					1996:2002	HCT-116	1996:2002	HCT-116	1996:2002	The EPS exhibited various degree of antitumor effect toward the tested cell lines (MCF-7, HCT-116 and HepG2).
29319513	12	49	theme	tested	1970:1975	arg1	MCF-7					1989:1993	MCF-7	1989:1993	MCF-7	1989:1993	The EPS exhibited various degree of antitumor effect toward the tested cell lines (MCF-7, HCT-116 and HepG2).
29319513	12	49	theme	tested	1970:1975	arg1	lines					1982:1986	the tested cell lines	1966:1986	the tested cell lines (MCF-7, HCT-116 and HepG2)	1966:2013	The EPS exhibited various degree of antitumor effect toward the tested cell lines (MCF-7, HCT-116 and HepG2).
29319513	4	50	theme	period	711:716	arg1	Effect					677:682	Effect	677:682	Effect of medium type, incubation period and pH	677:723	Effect of medium type, incubation period and pH were studied using the one factor at a time experiments.
29319513	5	51	theme	highest	804:810	arg1	productivity					812:823	the highest productivity	800:823	the highest productivity (24 gl-1) of exopolysaccharides	800:855	It was shown that the highest productivity (24 gl-1) of exopolysaccharides was recorded by using yeast malt glucose medium with pH 9 at the fourth day of incubation.
29319513	5	51	theme	highest	804:810	arg1	gl-1					829:832	24 gl-1	826:832	24 gl-1	826:832	It was shown that the highest productivity (24 gl-1) of exopolysaccharides was recorded by using yeast malt glucose medium with pH 9 at the fourth day of incubation.
29319513	1	52	theme	sugar	197:201	arg1	residues					203:210	different sugar residues	187:210	different sugar residues	187:210	Exopolysaccharides (EPSs) are high molecular weight polymers consisting of different sugar residues they are preferable for replacing synthetic polymers as they are degradable and nontoxic.
29319513	4	53	theme	pH	722:723	arg1	Effect					677:682	Effect	677:682	Effect of medium type, incubation period and pH	677:723	Effect of medium type, incubation period and pH were studied using the one factor at a time experiments.
29319513	7	54	theme	optimized	1094:1102	arg1	medium					1104:1109	The finalized optimized medium	1080:1109	The finalized optimized medium contained (gl-1) glucose (5), peptone (2.5), yeast extract (4.5) and malt extract (4.5)	1080:1197	The finalized optimized medium contained (gl-1) glucose (5), peptone (2.5), yeast extract (4.5) and malt extract (4.5) increased the production of EPS to 33.8 gl-1 with1.4 fold increase compared to the basal medium.
29319513	12	55	theme	antitumor	1942:1950	arg1	effect					1952:1957	antitumor effect	1942:1957	antitumor effect	1942:1957	The EPS exhibited various degree of antitumor effect toward the tested cell lines (MCF-7, HCT-116 and HepG2).
29319513	6	56	theme	Surface	983:989	arg1	RSM					1004:1006	RSM	1004:1006	RSM	1004:1006	Experimental design using Response Surface Methodology (RSM) was applied to optimize various nutrients at different concentrations.
29319513	6	56	theme	Surface	983:989	arg1	Methodology					991:1001	Response Surface Methodology	974:1001	Response Surface Methodology (RSM)	974:1007	Experimental design using Response Surface Methodology (RSM) was applied to optimize various nutrients at different concentrations.
29319513	1	57	dep	polymers	256:263	arg1	nontoxic					292:299	nontoxic	292:299	nontoxic	292:299	Exopolysaccharides (EPSs) are high molecular weight polymers consisting of different sugar residues they are preferable for replacing synthetic polymers as they are degradable and nontoxic.
29319513	1	57	dep	polymers	256:263	arg1	degradable					277:286	degradable	277:286	degradable	277:286	Exopolysaccharides (EPSs) are high molecular weight polymers consisting of different sugar residues they are preferable for replacing synthetic polymers as they are degradable and nontoxic.
29319513	4	58	theme	medium	687:692	arg1	type					694:697	medium type	687:697	medium type	687:697	Effect of medium type, incubation period and pH were studied using the one factor at a time experiments.
29319513	10	59	theme	polysaccharides	1588:1602	arg1	potentiality					1558:1569	the potentiality	1554:1569	the potentiality of the extracted polysaccharides in different medical applications	1554:1636	The study was extended to evaluate the potentiality of the extracted polysaccharides in different medical applications.
29319513	11	60	theme	antibacterial	1796:1808	arg1	activity					1810:1817	the highest antibacterial activity	1784:1817	the highest antibacterial activity (7.8, 9 and 10.4 AU/ml)	1784:1841	Results concluded that, EPS exhibited antibacterial activity against Aeromonas hydrophila, Pseudomonas aeruginosa and Streptococcus faecalis and the highest antibacterial activity (7.8, 9 and 10.4 AU/ml) was against S. faecalis at 50, 100 and 200 mg/ml respectively.
29319513	11	60	theme	antibacterial	1796:1808	arg1	AU/ml					1836:1840	7.8, 9 and 10.4 AU/ml	1820:1840	7.8, 9 and 10.4 AU/ml	1820:1840	Results concluded that, EPS exhibited antibacterial activity against Aeromonas hydrophila, Pseudomonas aeruginosa and Streptococcus faecalis and the highest antibacterial activity (7.8, 9 and 10.4 AU/ml) was against S. faecalis at 50, 100 and 200 mg/ml respectively.
29319513	3	61	theme	different	644:652	arg1	applications					663:674	different valuable applications	644:674	different valuable applications	644:674	The present study attempt to optimize the production of EPS by marine Bacillus subtilis SH1 in addition to characterization and investigation of different valuable applications.
29319513	0	62	from	Marine	82:87	arg1	Characterization					12:27	Characterization	12:27	Characterization	12:27	Production, Characterization and Valuable Applications of Exopolysaccharides from Marine Bacillus subtilis SH1.
29319513	0	62	from	Marine	82:87	arg1	Applications					42:53	Valuable Applications	33:53	Valuable Applications	33:53	Production, Characterization and Valuable Applications of Exopolysaccharides from Marine Bacillus subtilis SH1.
29319513	0	62	from	Marine	82:87	arg1	Production					0:9	Production	0:9	Production	0:9	Production, Characterization and Valuable Applications of Exopolysaccharides from Marine Bacillus subtilis SH1.
29319513	0	62	from	Marine	82:87	arg1	Exopolysaccharides					58:75	Exopolysaccharides	58:75	Exopolysaccharides from Marine	58:87	Production, Characterization and Valuable Applications of Exopolysaccharides from Marine Bacillus subtilis SH1.
29319513	14	63	theme	antioxidant	2080:2090	arg1	capacity					2092:2099	The antioxidant capacity	2076:2099	The antioxidant capacity	2076:2099	The antioxidant capacity increased with increasing the concentration of the sample.
29319513	15	64	theme	plasticized	2320:2330	arg1	film					2332:2335	plasticized film	2320:2335	plasticized film	2320:2335	Scanning electron microscopic analysis showed that EPS had compact film-like structure, which could make it a useful in the future applications as in preparing plasticized film.
29319513	5	65	theme	fourth	922:927	arg1	day					929:931	the fourth day	918:931	the fourth day of incubation	918:945	It was shown that the highest productivity (24 gl-1) of exopolysaccharides was recorded by using yeast malt glucose medium with pH 9 at the fourth day of incubation.
29319513	6	66	theme	Experimental	948:959	arg1	design					961:966	Experimental design	948:966	Experimental design using Response Surface Methodology (RSM)	948:1007	Experimental design using Response Surface Methodology (RSM) was applied to optimize various nutrients at different concentrations.
29319513	15	67	from	useful	2270:2275	arg1	applications					2291:2302	the future applications	2280:2302	the future applications as in preparing plasticized film	2280:2335	Scanning electron microscopic analysis showed that EPS had compact film-like structure, which could make it a useful in the future applications as in preparing plasticized film.
29319513	4	68	theme	factor	752:757	arg1	experiments					769:779	the one factor at a time experiments	744:779	the one factor at a time experiments	744:779	Effect of medium type, incubation period and pH were studied using the one factor at a time experiments.
29319513	15	69	theme	Scanning	2160:2167	arg1	analysis					2190:2197	Scanning electron microscopic analysis	2160:2197	Scanning electron microscopic analysis	2160:2197	Scanning electron microscopic analysis showed that EPS had compact film-like structure, which could make it a useful in the future applications as in preparing plasticized film.
29319513	15	70	theme	electron	2169:2176	arg1	analysis					2190:2197	Scanning electron microscopic analysis	2160:2197	Scanning electron microscopic analysis	2160:2197	Scanning electron microscopic analysis showed that EPS had compact film-like structure, which could make it a useful in the future applications as in preparing plasticized film.
29319513	3	71	theme	applications	663:674	arg1	investigation					627:639	investigation	627:639	investigation	627:639	The present study attempt to optimize the production of EPS by marine Bacillus subtilis SH1 in addition to characterization and investigation of different valuable applications.
29319513	3	71	theme	applications	663:674	arg1	characterization					606:621	characterization	606:621	characterization	606:621	The present study attempt to optimize the production of EPS by marine Bacillus subtilis SH1 in addition to characterization and investigation of different valuable applications.
29319513	1	72	theme	molecular	147:155	arg1	Exopolysaccharides					112:129	Exopolysaccharides	112:129	Exopolysaccharides (EPSs)	112:136	Exopolysaccharides (EPSs) are high molecular weight polymers consisting of different sugar residues they are preferable for replacing synthetic polymers as they are degradable and nontoxic.
29319513	1	72	theme	molecular	147:155	arg1	polymers					164:171	high molecular weight polymers	142:171	high molecular weight polymers consisting of different sugar residues	142:210	Exopolysaccharides (EPSs) are high molecular weight polymers consisting of different sugar residues they are preferable for replacing synthetic polymers as they are degradable and nontoxic.
29319513	15	73	from	applications	2291:2302	arg1	useful					2270:2275	useful	2270:2275	useful	2270:2275	Scanning electron microscopic analysis showed that EPS had compact film-like structure, which could make it a useful in the future applications as in preparing plasticized film.
29319513	7	74	theme	malt	1180:1183	arg1	extract					1185:1191	malt extract	1180:1191	malt extract (4.5)	1180:1197	The finalized optimized medium contained (gl-1) glucose (5), peptone (2.5), yeast extract (4.5) and malt extract (4.5) increased the production of EPS to 33.8 gl-1 with1.4 fold increase compared to the basal medium.
29319513	7	74	theme	malt	1180:1183	arg1	4.5					1194:1196	4.5	1194:1196	4.5	1194:1196	The finalized optimized medium contained (gl-1) glucose (5), peptone (2.5), yeast extract (4.5) and malt extract (4.5) increased the production of EPS to 33.8 gl-1 with1.4 fold increase compared to the basal medium.
29319513	0	75	from	Applications	42:53	arg1	Marine					82:87	Marine	82:87	Marine	82:87	Production, Characterization and Valuable Applications of Exopolysaccharides from Marine Bacillus subtilis SH1.
29319513	7	76	dep	contained	1111:1119	arg1	gl-1					1122:1125	gl-1	1122:1125	gl-1	1122:1125	The finalized optimized medium contained (gl-1) glucose (5), peptone (2.5), yeast extract (4.5) and malt extract (4.5) increased the production of EPS to 33.8 gl-1 with1.4 fold increase compared to the basal medium.
29319513	8	77	theme	Chemical	1296:1303	arg1	characterization					1305:1320	Chemical characterization	1296:1320	Chemical characterization of the extracted EPS	1296:1341	Chemical characterization of the extracted EPS showed that, FTIR spectra exhibited bands at various regions.
29319513	10	78	theme	medical	1617:1623	arg1	applications					1625:1636	different medical applications	1607:1636	different medical applications	1607:1636	The study was extended to evaluate the potentiality of the extracted polysaccharides in different medical applications.
29319513	7	79	theme	yeast	1156:1160	arg1	4.5					1171:1173	4.5	1171:1173	4.5	1171:1173	The finalized optimized medium contained (gl-1) glucose (5), peptone (2.5), yeast extract (4.5) and malt extract (4.5) increased the production of EPS to 33.8 gl-1 with1.4 fold increase compared to the basal medium.
29319513	7	79	theme	yeast	1156:1160	arg1	extract					1162:1168	yeast extract	1156:1168	yeast extract (4.5)	1156:1174	The finalized optimized medium contained (gl-1) glucose (5), peptone (2.5), yeast extract (4.5) and malt extract (4.5) increased the production of EPS to 33.8 gl-1 with1.4 fold increase compared to the basal medium.
29319513	5	80	theme	exopolysaccharides	838:855	arg1	productivity					812:823	the highest productivity	800:823	the highest productivity (24 gl-1) of exopolysaccharides	800:855	It was shown that the highest productivity (24 gl-1) of exopolysaccharides was recorded by using yeast malt glucose medium with pH 9 at the fourth day of incubation.
29319513	5	80	theme	exopolysaccharides	838:855	arg1	gl-1					829:832	24 gl-1	826:832	24 gl-1	826:832	It was shown that the highest productivity (24 gl-1) of exopolysaccharides was recorded by using yeast malt glucose medium with pH 9 at the fourth day of incubation.
29319513	5	81	from	day	929:931	arg1	medium					898:903	yeast malt glucose medium	879:903	yeast malt glucose medium with pH 9 at the fourth day of incubation	879:945	It was shown that the highest productivity (24 gl-1) of exopolysaccharides was recorded by using yeast malt glucose medium with pH 9 at the fourth day of incubation.
29319513	7	82	theme	fold	1252:1255	arg1	increase					1257:1264	33.8 gl-1 with1.4 fold increase	1234:1264	33.8 gl-1 with1.4 fold increase compared to the basal medium	1234:1293	The finalized optimized medium contained (gl-1) glucose (5), peptone (2.5), yeast extract (4.5) and malt extract (4.5) increased the production of EPS to 33.8 gl-1 with1.4 fold increase compared to the basal medium.
29319513	8	83	theme	extracted	1329:1337	arg1	EPS					1339:1341	the extracted EPS	1325:1341	the extracted EPS	1325:1341	Chemical characterization of the extracted EPS showed that, FTIR spectra exhibited bands at various regions.
29319513	2	84	theme	potential	455:463	arg1	applications					465:476	potential applications	455:476	potential applications	455:476	Many microorganisms possess the ability to synthesize and excrete exopolysaccharides with novel chemical compositions, properties and structures to have potential applications in different fields.
29319513	15	85	theme	film-like	2227:2235	arg1	structure					2237:2245	compact film-like structure	2219:2245	compact film-like structure	2219:2245	Scanning electron microscopic analysis showed that EPS had compact film-like structure, which could make it a useful in the future applications as in preparing plasticized film.
29319513	7	86	theme	gl-1	1239:1242	arg1	increase					1257:1264	33.8 gl-1 with1.4 fold increase	1234:1264	33.8 gl-1 with1.4 fold increase compared to the basal medium	1234:1293	The finalized optimized medium contained (gl-1) glucose (5), peptone (2.5), yeast extract (4.5) and malt extract (4.5) increased the production of EPS to 33.8 gl-1 with1.4 fold increase compared to the basal medium.
29319513	3	87	theme	present	503:509	arg1	attempt					517:523	The present study attempt	499:523	The present study attempt	499:523	The present study attempt to optimize the production of EPS by marine Bacillus subtilis SH1 in addition to characterization and investigation of different valuable applications.
29319513	0	88	theme	Exopolysaccharides	58:75	arg1	Characterization					12:27	Characterization	12:27	Characterization	12:27	Production, Characterization and Valuable Applications of Exopolysaccharides from Marine Bacillus subtilis SH1.
29319513	0	88	theme	Exopolysaccharides	58:75	arg1	Applications					42:53	Valuable Applications	33:53	Valuable Applications	33:53	Production, Characterization and Valuable Applications of Exopolysaccharides from Marine Bacillus subtilis SH1.
29319513	0	88	theme	Exopolysaccharides	58:75	arg1	Production					0:9	Production	0:9	Production	0:9	Production, Characterization and Valuable Applications of Exopolysaccharides from Marine Bacillus subtilis SH1.
29319513	4	89	from	time	764:767	arg1	factor					752:757	the one factor	744:757	the one factor at a time experiments	744:779	Effect of medium type, incubation period and pH were studied using the one factor at a time experiments.
29319513	12	90	dep	lines	1982:1986	arg1	HepG2					2008:2012	HepG2	2008:2012	HepG2	2008:2012	The EPS exhibited various degree of antitumor effect toward the tested cell lines (MCF-7, HCT-116 and HepG2).
29319513	12	90	dep	lines	1982:1986	arg1	HCT-116					1996:2002	HCT-116	1996:2002	HCT-116	1996:2002	The EPS exhibited various degree of antitumor effect toward the tested cell lines (MCF-7, HCT-116 and HepG2).
29319513	12	90	dep	lines	1982:1986	arg1	MCF-7					1989:1993	MCF-7	1989:1993	MCF-7	1989:1993	The EPS exhibited various degree of antitumor effect toward the tested cell lines (MCF-7, HCT-116 and HepG2).
29319513	12	90	dep	lines	1982:1986	arg1	lines					1982:1986	the tested cell lines	1966:1986	the tested cell lines (MCF-7, HCT-116 and HepG2)	1966:2013	The EPS exhibited various degree of antitumor effect toward the tested cell lines (MCF-7, HCT-116 and HepG2).
29319513	15	91	theme	microscopic	2178:2188	arg1	analysis					2190:2197	Scanning electron microscopic analysis	2160:2197	Scanning electron microscopic analysis	2160:2197	Scanning electron microscopic analysis showed that EPS had compact film-like structure, which could make it a useful in the future applications as in preparing plasticized film.
29319513	2	92	theme	chemical	398:405	arg1	compositions					407:418	novel chemical compositions	392:418	novel chemical compositions	392:418	Many microorganisms possess the ability to synthesize and excrete exopolysaccharides with novel chemical compositions, properties and structures to have potential applications in different fields.
29319513	1	93	theme	synthetic	246:254	arg1	polymers					256:263	synthetic polymers	246:263	synthetic polymers as they are degradable and nontoxic	246:299	Exopolysaccharides (EPSs) are high molecular weight polymers consisting of different sugar residues they are preferable for replacing synthetic polymers as they are degradable and nontoxic.
29319513	3	94	theme	marine	562:567	arg1	SH1					587:589	marine Bacillus subtilis SH1	562:589	marine Bacillus subtilis SH1 in addition to characterization and investigation of different valuable applications	562:674	The present study attempt to optimize the production of EPS by marine Bacillus subtilis SH1 in addition to characterization and investigation of different valuable applications.
25928444	6	0	theme	first	851:855	arg1	This					839:842	This	839:842	This	839:842	This is the first report for a gelatin-matrix composite containing cellulose.
25928444	6	0	theme	first	851:855	arg1	report					857:862	the first report	847:862	the first report for a gelatin-matrix composite containing cellulose	847:914	This is the first report for a gelatin-matrix composite containing cellulose.
25928444	10	1	theme	UV	1570:1571	arg1	coating					1587:1593	a UV blocking food coating	1568:1593	a UV blocking food coating	1568:1593	The potential use of these composite materials as a UV blocking food coating is discussed.
25928444	8	2	theme	gelatin	1387:1393	arg1	matrix					1395:1400	the gelatin matrix	1383:1400	the gelatin matrix	1383:1400	The average initial Raman band shift rate value corresponds to an average effective Young's modulus of 39 ± 13 GPa and 73 ± 25 GPa, respectively, for either a 2D and 3D network of BC fibrils embedded in the gelatin matrix.
25928444	7	3	theme	shift	1042:1046	arg1	rate					1048:1051	an average initial shift rate	1023:1051	an average initial shift rate with respect to strain of -0.63 ± 0.2 cm(-1)%(-1)	1023:1101	As a function of strain, two distinct domains, both showing linear relationships, were observed for which an average initial shift rate with respect to strain of -0.63 ± 0.2 cm(-1)%(-1) was observed, followed by an average shift rate of -0.25 ± 0.03 cm(-1)%(-1).
25928444	2	4	theme	scanning	304:311	arg1	microscopy					322:331	scanning electron microscopy	304:331	scanning electron microscopy	304:331	Composite morphology was studied using scanning electron microscopy and 2D Raman imaging revealing an inhomogeneous dispersion of BC within the gelatin matrix.
25928444	2	5	theme	gelatin	409:415	arg1	matrix					417:422	the gelatin matrix	405:422	the gelatin matrix	405:422	Composite morphology was studied using scanning electron microscopy and 2D Raman imaging revealing an inhomogeneous dispersion of BC within the gelatin matrix.
25928444	7	6	theme	average	1026:1032	arg1	rate					1048:1051	an average initial shift rate	1023:1051	an average initial shift rate with respect to strain of -0.63 ± 0.2 cm(-1)%(-1)	1023:1101	As a function of strain, two distinct domains, both showing linear relationships, were observed for which an average initial shift rate with respect to strain of -0.63 ± 0.2 cm(-1)%(-1) was observed, followed by an average shift rate of -0.25 ± 0.03 cm(-1)%(-1).
25928444	5	7	theme	transfer	744:751	arg1	occurring					753:761	stress transfer occurring	737:761	stress transfer occurring between the gelatin and BC fibrils	737:796	Additionally, stress transfer occurring between the gelatin and BC fibrils was quantified using Raman spectroscopy.
25928444	9	8	theme	stress	1420:1425	arg1	relationship					1437:1448	a linear relationship	1428:1448	a linear relationship	1428:1448	As a function of stress, a linear relationship was observed with a Raman band shift rate of -27 ± 3 cm(-1)GPa(-1).
25928444	9	8	theme	stress	1420:1425	arg1	function					1408:1415	a function	1406:1415	a function of stress	1406:1425	As a function of stress, a linear relationship was observed with a Raman band shift rate of -27 ± 3 cm(-1)GPa(-1).
25928444	2	9	theme	2D	337:338	arg1	imaging					346:352	2D Raman imaging	337:352	2D Raman imaging	337:352	Composite morphology was studied using scanning electron microscopy and 2D Raman imaging revealing an inhomogeneous dispersion of BC within the gelatin matrix.
25928444	4	10	theme	=	625:625	arg1	nm					631:632	λ = 550 nm	623:632	λ = 550 nm	623:632	By adding a 10 wt % fraction of BC in gelatin, visible (λ = 550 nm) and UV (λ = 350 nm) transmittances were found to decrease by ∼35 and 40%, respectively.
25928444	5	11	theme	gelatin	775:781	arg1	fibrils					790:796	the gelatin and BC fibrils	771:796	fibrils	790:796	Additionally, stress transfer occurring between the gelatin and BC fibrils was quantified using Raman spectroscopy.
25928444	8	12	theme	average	1246:1252	arg1	modulus					1272:1278	an average effective Young's modulus	1243:1278	an average effective Young's modulus of 39 ± 13 GPa and 73 ± 25 GPa, respectively, for either a 2D and 3D network of BC fibrils embedded in the gelatin matrix	1243:1400	The average initial Raman band shift rate value corresponds to an average effective Young's modulus of 39 ± 13 GPa and 73 ± 25 GPa, respectively, for either a 2D and 3D network of BC fibrils embedded in the gelatin matrix.
25928444	7	13	theme	distinct	946:953	arg1	function					922:929	a function	920:929	a function of strain	920:939	As a function of strain, two distinct domains, both showing linear relationships, were observed for which an average initial shift rate with respect to strain of -0.63 ± 0.2 cm(-1)%(-1) was observed, followed by an average shift rate of -0.25 ± 0.03 cm(-1)%(-1).
25928444	7	13	theme	distinct	946:953	arg1	domains					955:961	two distinct domains	942:961	two distinct domains	942:961	As a function of strain, two distinct domains, both showing linear relationships, were observed for which an average initial shift rate with respect to strain of -0.63 ± 0.2 cm(-1)%(-1) was observed, followed by an average shift rate of -0.25 ± 0.03 cm(-1)%(-1).
25928444	5	14	theme	BC	787:788	arg1	fibrils					790:796	the gelatin and BC fibrils	771:796	fibrils	790:796	Additionally, stress transfer occurring between the gelatin and BC fibrils was quantified using Raman spectroscopy.
25928444	6	15	theme	gelatin-matrix	870:883	arg1	composite					885:893	a gelatin-matrix composite	868:893	a gelatin-matrix composite containing cellulose	868:914	This is the first report for a gelatin-matrix composite containing cellulose.
25928444	8	16	theme	Young	1264:1268	arg1	modulus					1272:1278	an average effective Young's modulus	1243:1278	an average effective Young's modulus of 39 ± 13 GPa and 73 ± 25 GPa, respectively, for either a 2D and 3D network of BC fibrils embedded in the gelatin matrix	1243:1400	The average initial Raman band shift rate value corresponds to an average effective Young's modulus of 39 ± 13 GPa and 73 ± 25 GPa, respectively, for either a 2D and 3D network of BC fibrils embedded in the gelatin matrix.
25928444	7	17	theme	linear	977:982	arg1	relationships					984:996	showing linear relationships	969:996	showing linear relationships	969:996	As a function of strain, two distinct domains, both showing linear relationships, were observed for which an average initial shift rate with respect to strain of -0.63 ± 0.2 cm(-1)%(-1) was observed, followed by an average shift rate of -0.25 ± 0.03 cm(-1)%(-1).
25928444	8	18	from	embedded	1371:1378	arg1	matrix					1395:1400	the gelatin matrix	1383:1400	the gelatin matrix	1383:1400	The average initial Raman band shift rate value corresponds to an average effective Young's modulus of 39 ± 13 GPa and 73 ± 25 GPa, respectively, for either a 2D and 3D network of BC fibrils embedded in the gelatin matrix.
25928444	9	19	theme	band	1476:1479	arg1	rate					1487:1490	a Raman band shift rate	1468:1490	a Raman band shift rate of -27 ± 3 cm(-1)GPa(-1)	1468:1515	As a function of stress, a linear relationship was observed with a Raman band shift rate of -27 ± 3 cm(-1)GPa(-1).
25928444	7	20	theme	%	1173:1173	arg1	rate					1146:1149	an average shift rate	1129:1149	an average shift rate of -0.25 ± 0.03 cm(-1)%(-1)	1129:1177	As a function of strain, two distinct domains, both showing linear relationships, were observed for which an average initial shift rate with respect to strain of -0.63 ± 0.2 cm(-1)%(-1) was observed, followed by an average shift rate of -0.25 ± 0.03 cm(-1)%(-1).
25928444	4	21	theme	=	645:645	arg1	nm					651:652	λ = 350 nm	643:652	λ = 350 nm	643:652	By adding a 10 wt % fraction of BC in gelatin, visible (λ = 550 nm) and UV (λ = 350 nm) transmittances were found to decrease by ∼35 and 40%, respectively.
25928444	4	21	theme	=	645:645	arg1	UV					639:640	UV	639:640	UV (λ = 350 nm)	639:653	By adding a 10 wt % fraction of BC in gelatin, visible (λ = 550 nm) and UV (λ = 350 nm) transmittances were found to decrease by ∼35 and 40%, respectively.
25928444	0	22	theme	Tunable	73:79	arg1	Properties					89:98	Tunable Optical Properties	73:98	Tunable Optical Properties	73:98	Stress Transfer Quantification in Gelatin-Matrix Natural Composites with Tunable Optical Properties.
25928444	1	23	theme	natural	162:168	arg1	materials					180:188	natural composite materials	162:188	natural composite materials prepared from bacterial cellulose (BC) incorporated into a gelatin matrix	162:262	This work reports on the preparation and characterization of natural composite materials prepared from bacterial cellulose (BC) incorporated into a gelatin matrix.
25928444	0	24	from	Quantification	16:29	arg1	Composites					57:66	Gelatin-Matrix Natural Composites	34:66	Gelatin-Matrix Natural Composites with Tunable Optical Properties	34:98	Stress Transfer Quantification in Gelatin-Matrix Natural Composites with Tunable Optical Properties.
25928444	1	25	theme	materials	180:188	arg1	characterization					142:157	characterization	142:157	characterization	142:157	This work reports on the preparation and characterization of natural composite materials prepared from bacterial cellulose (BC) incorporated into a gelatin matrix.
25928444	1	25	theme	materials	180:188	arg1	preparation					126:136	preparation	126:136	preparation	126:136	This work reports on the preparation and characterization of natural composite materials prepared from bacterial cellulose (BC) incorporated into a gelatin matrix.
25928444	7	26	theme	%	1097:1097	arg1	strain					1069:1074	strain	1069:1074	strain of -0.63 ± 0.2 cm(-1)%(-1)	1069:1101	As a function of strain, two distinct domains, both showing linear relationships, were observed for which an average initial shift rate with respect to strain of -0.63 ± 0.2 cm(-1)%(-1) was observed, followed by an average shift rate of -0.25 ± 0.03 cm(-1)%(-1).
25928444	8	27	theme	±	1302:1302	arg1	GPa					1307:1309	73 ± 25 GPa	1299:1309	73 ± 25 GPa	1299:1309	The average initial Raman band shift rate value corresponds to an average effective Young's modulus of 39 ± 13 GPa and 73 ± 25 GPa, respectively, for either a 2D and 3D network of BC fibrils embedded in the gelatin matrix.
25928444	3	28	dep	light	528:532	arg1	to					522:523	to	522:523	to	522:523	The composite materials showed controllable degrees of transparency to visible light and opacity to UV light depending on BC weight fraction.
25928444	8	29	theme	GPa	1307:1309	arg1	modulus					1272:1278	an average effective Young's modulus	1243:1278	an average effective Young's modulus of 39 ± 13 GPa and 73 ± 25 GPa, respectively, for either a 2D and 3D network of BC fibrils embedded in the gelatin matrix	1243:1400	The average initial Raman band shift rate value corresponds to an average effective Young's modulus of 39 ± 13 GPa and 73 ± 25 GPa, respectively, for either a 2D and 3D network of BC fibrils embedded in the gelatin matrix.
25928444	7	30	dep	domains	955:961	arg1	both					964:967	both	964:967	both	964:967	As a function of strain, two distinct domains, both showing linear relationships, were observed for which an average initial shift rate with respect to strain of -0.63 ± 0.2 cm(-1)%(-1) was observed, followed by an average shift rate of -0.25 ± 0.03 cm(-1)%(-1).
25928444	3	31	theme	controllable	456:467	arg1	degrees					469:475	controllable degrees	456:475	controllable degrees of transparency	456:491	The composite materials showed controllable degrees of transparency to visible light and opacity to UV light depending on BC weight fraction.
25928444	0	32	theme	Stress	0:5	arg1	Quantification					16:29	Stress Transfer Quantification	0:29	Stress Transfer Quantification in Gelatin-Matrix Natural Composites with Tunable Optical Properties.	0:99	Stress Transfer Quantification in Gelatin-Matrix Natural Composites with Tunable Optical Properties.
25928444	2	33	theme	BC	395:396	arg1	dispersion					381:390	an inhomogeneous dispersion	364:390	an inhomogeneous dispersion of BC within the gelatin matrix	364:422	Composite morphology was studied using scanning electron microscopy and 2D Raman imaging revealing an inhomogeneous dispersion of BC within the gelatin matrix.
25928444	8	34	theme	initial	1192:1198	arg1	value					1222:1226	The average initial Raman band shift rate value	1180:1226	The average initial Raman band shift rate value	1180:1226	The average initial Raman band shift rate value corresponds to an average effective Young's modulus of 39 ± 13 GPa and 73 ± 25 GPa, respectively, for either a 2D and 3D network of BC fibrils embedded in the gelatin matrix.
25928444	6	35	contain	containing	895:904	arg2	cellulose					906:914	cellulose	906:914	cellulose	906:914	This is the first report for a gelatin-matrix composite containing cellulose.
25928444	6	35	contain	containing	895:904	arg1	composite					885:893	a gelatin-matrix composite	868:893	a gelatin-matrix composite containing cellulose	868:914	This is the first report for a gelatin-matrix composite containing cellulose.
25928444	7	36	theme	±	1085:1085	arg1	-1					1099:1100	-1	1099:1100	-1	1099:1100	As a function of strain, two distinct domains, both showing linear relationships, were observed for which an average initial shift rate with respect to strain of -0.63 ± 0.2 cm(-1)%(-1) was observed, followed by an average shift rate of -0.25 ± 0.03 cm(-1)%(-1).
25928444	7	36	theme	±	1085:1085	arg1	%					1097:1097	-0.63 ± 0.2 cm(-1)%	1079:1097	-0.63 ± 0.2 cm(-1)%(-1)	1079:1101	As a function of strain, two distinct domains, both showing linear relationships, were observed for which an average initial shift rate with respect to strain of -0.63 ± 0.2 cm(-1)%(-1) was observed, followed by an average shift rate of -0.25 ± 0.03 cm(-1)%(-1).
25928444	4	37	theme	wt	582:583	arg1	fraction					587:594	a 10 wt % fraction	577:594	a 10 wt % fraction of BC	577:600	By adding a 10 wt % fraction of BC in gelatin, visible (λ = 550 nm) and UV (λ = 350 nm) transmittances were found to decrease by ∼35 and 40%, respectively.
25928444	7	38	theme	cm	1091:1092	arg1	-1					1099:1100	-1	1099:1100	-1	1099:1100	As a function of strain, two distinct domains, both showing linear relationships, were observed for which an average initial shift rate with respect to strain of -0.63 ± 0.2 cm(-1)%(-1) was observed, followed by an average shift rate of -0.25 ± 0.03 cm(-1)%(-1).
25928444	7	38	theme	cm	1091:1092	arg1	%					1097:1097	-0.63 ± 0.2 cm(-1)%	1079:1097	-0.63 ± 0.2 cm(-1)%(-1)	1079:1101	As a function of strain, two distinct domains, both showing linear relationships, were observed for which an average initial shift rate with respect to strain of -0.63 ± 0.2 cm(-1)%(-1) was observed, followed by an average shift rate of -0.25 ± 0.03 cm(-1)%(-1).
25928444	8	39	theme	band	1206:1209	arg1	value					1222:1226	The average initial Raman band shift rate value	1180:1226	The average initial Raman band shift rate value	1180:1226	The average initial Raman band shift rate value corresponds to an average effective Young's modulus of 39 ± 13 GPa and 73 ± 25 GPa, respectively, for either a 2D and 3D network of BC fibrils embedded in the gelatin matrix.
25928444	0	40	theme	Gelatin-Matrix	34:47	arg1	Composites					57:66	Gelatin-Matrix Natural Composites	34:66	Gelatin-Matrix Natural Composites with Tunable Optical Properties	34:98	Stress Transfer Quantification in Gelatin-Matrix Natural Composites with Tunable Optical Properties.
25928444	8	41	theme	rate	1217:1220	arg1	value					1222:1226	The average initial Raman band shift rate value	1180:1226	The average initial Raman band shift rate value	1180:1226	The average initial Raman band shift rate value corresponds to an average effective Young's modulus of 39 ± 13 GPa and 73 ± 25 GPa, respectively, for either a 2D and 3D network of BC fibrils embedded in the gelatin matrix.
25928444	3	42	theme	UV	525:526	arg1	light					528:532	opacity to UV light	514:532	opacity to UV light	514:532	The composite materials showed controllable degrees of transparency to visible light and opacity to UV light depending on BC weight fraction.
25928444	10	43	theme	materials	1555:1563	arg1	use					1532:1534	The potential use	1518:1534	The potential use of these composite materials as a UV blocking food coating	1518:1593	The potential use of these composite materials as a UV blocking food coating is discussed.
25928444	3	44	theme	opacity	514:520	arg1	light					528:532	opacity to UV light	514:532	opacity to UV light	514:532	The composite materials showed controllable degrees of transparency to visible light and opacity to UV light depending on BC weight fraction.
25928444	7	45	theme	average	1132:1138	arg1	rate					1146:1149	an average shift rate	1129:1149	an average shift rate of -0.25 ± 0.03 cm(-1)%(-1)	1129:1177	As a function of strain, two distinct domains, both showing linear relationships, were observed for which an average initial shift rate with respect to strain of -0.63 ± 0.2 cm(-1)%(-1) was observed, followed by an average shift rate of -0.25 ± 0.03 cm(-1)%(-1).
25928444	0	46	with	Composites	57:66	arg1	Properties					89:98	Tunable Optical Properties	73:98	Tunable Optical Properties	73:98	Stress Transfer Quantification in Gelatin-Matrix Natural Composites with Tunable Optical Properties.
25928444	3	47	theme	BC	547:548	arg1	fraction					557:564	BC weight fraction	547:564	BC weight fraction	547:564	The composite materials showed controllable degrees of transparency to visible light and opacity to UV light depending on BC weight fraction.
25928444	1	48	theme	gelatin	249:255	arg1	matrix					257:262	a gelatin matrix	247:262	a gelatin matrix	247:262	This work reports on the preparation and characterization of natural composite materials prepared from bacterial cellulose (BC) incorporated into a gelatin matrix.
25928444	8	49	theme	BC	1360:1361	arg1	fibrils					1363:1369	BC fibrils	1360:1369	BC fibrils embedded in the gelatin matrix	1360:1400	The average initial Raman band shift rate value corresponds to an average effective Young's modulus of 39 ± 13 GPa and 73 ± 25 GPa, respectively, for either a 2D and 3D network of BC fibrils embedded in the gelatin matrix.
25928444	10	50	theme	blocking	1573:1580	arg1	coating					1587:1593	a UV blocking food coating	1568:1593	a UV blocking food coating	1568:1593	The potential use of these composite materials as a UV blocking food coating is discussed.
25928444	8	51	theme	embedded	1371:1378	arg1	fibrils					1363:1369	BC fibrils	1360:1369	BC fibrils embedded in the gelatin matrix	1360:1400	The average initial Raman band shift rate value corresponds to an average effective Young's modulus of 39 ± 13 GPa and 73 ± 25 GPa, respectively, for either a 2D and 3D network of BC fibrils embedded in the gelatin matrix.
25928444	7	52	theme	initial	1034:1040	arg1	rate					1048:1051	an average initial shift rate	1023:1051	an average initial shift rate with respect to strain of -0.63 ± 0.2 cm(-1)%(-1)	1023:1101	As a function of strain, two distinct domains, both showing linear relationships, were observed for which an average initial shift rate with respect to strain of -0.63 ± 0.2 cm(-1)%(-1) was observed, followed by an average shift rate of -0.25 ± 0.03 cm(-1)%(-1).
25928444	2	53	theme	electron	313:320	arg1	microscopy					322:331	scanning electron microscopy	304:331	scanning electron microscopy	304:331	Composite morphology was studied using scanning electron microscopy and 2D Raman imaging revealing an inhomogeneous dispersion of BC within the gelatin matrix.
25928444	4	54	theme	BC	599:600	arg1	fraction					587:594	a 10 wt % fraction	577:594	a 10 wt % fraction of BC	577:600	By adding a 10 wt % fraction of BC in gelatin, visible (λ = 550 nm) and UV (λ = 350 nm) transmittances were found to decrease by ∼35 and 40%, respectively.
25928444	2	55	theme	inhomogeneous	367:379	arg1	dispersion					381:390	an inhomogeneous dispersion	364:390	an inhomogeneous dispersion of BC within the gelatin matrix	364:422	Composite morphology was studied using scanning electron microscopy and 2D Raman imaging revealing an inhomogeneous dispersion of BC within the gelatin matrix.
25928444	2	56	theme	Raman	340:344	arg1	imaging					346:352	2D Raman imaging	337:352	2D Raman imaging	337:352	Composite morphology was studied using scanning electron microscopy and 2D Raman imaging revealing an inhomogeneous dispersion of BC within the gelatin matrix.
25928444	1	57	theme	bacterial	204:212	arg1	BC					225:226	BC	225:226	BC	225:226	This work reports on the preparation and characterization of natural composite materials prepared from bacterial cellulose (BC) incorporated into a gelatin matrix.
25928444	1	57	theme	bacterial	204:212	arg1	cellulose					214:222	bacterial cellulose	204:222	bacterial cellulose (BC) incorporated into a gelatin matrix	204:262	This work reports on the preparation and characterization of natural composite materials prepared from bacterial cellulose (BC) incorporated into a gelatin matrix.
25928444	4	58	theme	UV	639:640	arg1	transmittances					655:668	visible (λ = 550 nm) and UV (λ = 350 nm) transmittances	614:668	visible (λ = 550 nm) and UV (λ = 350 nm) transmittances	614:668	By adding a 10 wt % fraction of BC in gelatin, visible (λ = 550 nm) and UV (λ = 350 nm) transmittances were found to decrease by ∼35 and 40%, respectively.
25928444	9	59	theme	linear	1430:1435	arg1	relationship					1437:1448	a linear relationship	1428:1448	a linear relationship	1428:1448	As a function of stress, a linear relationship was observed with a Raman band shift rate of -27 ± 3 cm(-1)GPa(-1).
25928444	9	59	theme	linear	1430:1435	arg1	function					1408:1415	a function	1406:1415	a function of stress	1406:1425	As a function of stress, a linear relationship was observed with a Raman band shift rate of -27 ± 3 cm(-1)GPa(-1).
25928444	8	60	theme	effective	1254:1262	arg1	modulus					1272:1278	an average effective Young's modulus	1243:1278	an average effective Young's modulus of 39 ± 13 GPa and 73 ± 25 GPa, respectively, for either a 2D and 3D network of BC fibrils embedded in the gelatin matrix	1243:1400	The average initial Raman band shift rate value corresponds to an average effective Young's modulus of 39 ± 13 GPa and 73 ± 25 GPa, respectively, for either a 2D and 3D network of BC fibrils embedded in the gelatin matrix.
25928444	4	61	theme	λ	623:623	arg1	nm					631:632	λ = 550 nm	623:632	λ = 550 nm	623:632	By adding a 10 wt % fraction of BC in gelatin, visible (λ = 550 nm) and UV (λ = 350 nm) transmittances were found to decrease by ∼35 and 40%, respectively.
25928444	7	62	theme	showing	969:975	arg1	relationships					984:996	showing linear relationships	969:996	showing linear relationships	969:996	As a function of strain, two distinct domains, both showing linear relationships, were observed for which an average initial shift rate with respect to strain of -0.63 ± 0.2 cm(-1)%(-1) was observed, followed by an average shift rate of -0.25 ± 0.03 cm(-1)%(-1).
25928444	4	63	dep	visible	614:620	arg1	nm					631:632	λ = 550 nm	623:632	λ = 550 nm	623:632	By adding a 10 wt % fraction of BC in gelatin, visible (λ = 550 nm) and UV (λ = 350 nm) transmittances were found to decrease by ∼35 and 40%, respectively.
25928444	9	64	theme	Raman	1470:1474	arg1	rate					1487:1490	a Raman band shift rate	1468:1490	a Raman band shift rate of -27 ± 3 cm(-1)GPa(-1)	1468:1515	As a function of stress, a linear relationship was observed with a Raman band shift rate of -27 ± 3 cm(-1)GPa(-1).
25928444	8	65	theme	±	1286:1286	arg1	GPa					1291:1293	39 ± 13 GPa	1283:1293	39 ± 13 GPa	1283:1293	The average initial Raman band shift rate value corresponds to an average effective Young's modulus of 39 ± 13 GPa and 73 ± 25 GPa, respectively, for either a 2D and 3D network of BC fibrils embedded in the gelatin matrix.
25928444	0	66	theme	Optical	81:87	arg1	Properties					89:98	Tunable Optical Properties	73:98	Tunable Optical Properties	73:98	Stress Transfer Quantification in Gelatin-Matrix Natural Composites with Tunable Optical Properties.
25928444	2	67	theme	Composite	265:273	arg1	morphology					275:284	Composite morphology	265:284	Composite morphology	265:284	Composite morphology was studied using scanning electron microscopy and 2D Raman imaging revealing an inhomogeneous dispersion of BC within the gelatin matrix.
25928444	7	68	theme	strain	934:939	arg1	function					922:929	a function	920:929	a function of strain	920:939	As a function of strain, two distinct domains, both showing linear relationships, were observed for which an average initial shift rate with respect to strain of -0.63 ± 0.2 cm(-1)%(-1) was observed, followed by an average shift rate of -0.25 ± 0.03 cm(-1)%(-1).
25928444	7	68	theme	strain	934:939	arg1	domains					955:961	two distinct domains	942:961	two distinct domains	942:961	As a function of strain, two distinct domains, both showing linear relationships, were observed for which an average initial shift rate with respect to strain of -0.63 ± 0.2 cm(-1)%(-1) was observed, followed by an average shift rate of -0.25 ± 0.03 cm(-1)%(-1).
25928444	5	69	theme	Raman	819:823	arg1	spectroscopy					825:836	Raman spectroscopy	819:836	Raman spectroscopy	819:836	Additionally, stress transfer occurring between the gelatin and BC fibrils was quantified using Raman spectroscopy.
25928444	9	70	theme	shift	1481:1485	arg1	rate					1487:1490	a Raman band shift rate	1468:1490	a Raman band shift rate of -27 ± 3 cm(-1)GPa(-1)	1468:1515	As a function of stress, a linear relationship was observed with a Raman band shift rate of -27 ± 3 cm(-1)GPa(-1).
25928444	8	71	theme	GPa	1291:1293	arg1	modulus					1272:1278	an average effective Young's modulus	1243:1278	an average effective Young's modulus of 39 ± 13 GPa and 73 ± 25 GPa, respectively, for either a 2D and 3D network of BC fibrils embedded in the gelatin matrix	1243:1400	The average initial Raman band shift rate value corresponds to an average effective Young's modulus of 39 ± 13 GPa and 73 ± 25 GPa, respectively, for either a 2D and 3D network of BC fibrils embedded in the gelatin matrix.
25928444	4	72	theme	λ	643:643	arg1	nm					651:652	λ = 350 nm	643:652	λ = 350 nm	643:652	By adding a 10 wt % fraction of BC in gelatin, visible (λ = 550 nm) and UV (λ = 350 nm) transmittances were found to decrease by ∼35 and 40%, respectively.
25928444	4	72	theme	λ	643:643	arg1	UV					639:640	UV	639:640	UV (λ = 350 nm)	639:653	By adding a 10 wt % fraction of BC in gelatin, visible (λ = 550 nm) and UV (λ = 350 nm) transmittances were found to decrease by ∼35 and 40%, respectively.
25928444	1	73	theme	composite	170:178	arg1	materials					180:188	natural composite materials	162:188	natural composite materials prepared from bacterial cellulose (BC) incorporated into a gelatin matrix	162:262	This work reports on the preparation and characterization of natural composite materials prepared from bacterial cellulose (BC) incorporated into a gelatin matrix.
25928444	9	74	theme	±	1499:1499	arg1	-1					1513:1514	-1	1513:1514	-1	1513:1514	As a function of stress, a linear relationship was observed with a Raman band shift rate of -27 ± 3 cm(-1)GPa(-1).
25928444	9	74	theme	±	1499:1499	arg1	GPa					1509:1511	-27 ± 3 cm(-1)GPa	1495:1511	-27 ± 3 cm(-1)GPa(-1)	1495:1515	As a function of stress, a linear relationship was observed with a Raman band shift rate of -27 ± 3 cm(-1)GPa(-1).
25928444	9	75	theme	cm	1503:1504	arg1	-1					1513:1514	-1	1513:1514	-1	1513:1514	As a function of stress, a linear relationship was observed with a Raman band shift rate of -27 ± 3 cm(-1)GPa(-1).
25928444	9	75	theme	cm	1503:1504	arg1	GPa					1509:1511	-27 ± 3 cm(-1)GPa	1495:1511	-27 ± 3 cm(-1)GPa(-1)	1495:1515	As a function of stress, a linear relationship was observed with a Raman band shift rate of -27 ± 3 cm(-1)GPa(-1).
25928444	1	76	dep	preparation	126:136	arg1	the					122:124	the	122:124	the	122:124	This work reports on the preparation and characterization of natural composite materials prepared from bacterial cellulose (BC) incorporated into a gelatin matrix.
25928444	3	77	theme	transparency	480:491	arg1	degrees					469:475	controllable degrees	456:475	controllable degrees of transparency	456:491	The composite materials showed controllable degrees of transparency to visible light and opacity to UV light depending on BC weight fraction.
25928444	5	78	theme	stress	737:742	arg1	occurring					753:761	stress transfer occurring	737:761	stress transfer occurring between the gelatin and BC fibrils	737:796	Additionally, stress transfer occurring between the gelatin and BC fibrils was quantified using Raman spectroscopy.
25928444	8	79	dep	network	1349:1355	arg1	either					1330:1335	either	1330:1335	either	1330:1335	The average initial Raman band shift rate value corresponds to an average effective Young's modulus of 39 ± 13 GPa and 73 ± 25 GPa, respectively, for either a 2D and 3D network of BC fibrils embedded in the gelatin matrix.
25928444	7	80	dep	observed	1107:1114	arg1	followed					1117:1124	followed	1117:1124	followed by an average shift rate of -0.25 ± 0.03 cm(-1)%(-1)	1117:1177	As a function of strain, two distinct domains, both showing linear relationships, were observed for which an average initial shift rate with respect to strain of -0.63 ± 0.2 cm(-1)%(-1) was observed, followed by an average shift rate of -0.25 ± 0.03 cm(-1)%(-1).
25928444	7	81	theme	cm	1167:1168	arg1	-1					1175:1176	-1	1175:1176	-1	1175:1176	As a function of strain, two distinct domains, both showing linear relationships, were observed for which an average initial shift rate with respect to strain of -0.63 ± 0.2 cm(-1)%(-1) was observed, followed by an average shift rate of -0.25 ± 0.03 cm(-1)%(-1).
25928444	7	81	theme	cm	1167:1168	arg1	%					1173:1173	-0.25 ± 0.03 cm(-1)%	1154:1173	-0.25 ± 0.03 cm(-1)%(-1)	1154:1177	As a function of strain, two distinct domains, both showing linear relationships, were observed for which an average initial shift rate with respect to strain of -0.63 ± 0.2 cm(-1)%(-1) was observed, followed by an average shift rate of -0.25 ± 0.03 cm(-1)%(-1).
25928444	4	82	theme	10	579:580	arg1	wt					582:583	wt	582:583	wt	582:583	By adding a 10 wt % fraction of BC in gelatin, visible (λ = 550 nm) and UV (λ = 350 nm) transmittances were found to decrease by ∼35 and 40%, respectively.
25928444	7	83	theme	±	1160:1160	arg1	-1					1175:1176	-1	1175:1176	-1	1175:1176	As a function of strain, two distinct domains, both showing linear relationships, were observed for which an average initial shift rate with respect to strain of -0.63 ± 0.2 cm(-1)%(-1) was observed, followed by an average shift rate of -0.25 ± 0.03 cm(-1)%(-1).
25928444	7	83	theme	±	1160:1160	arg1	%					1173:1173	-0.25 ± 0.03 cm(-1)%	1154:1173	-0.25 ± 0.03 cm(-1)%(-1)	1154:1177	As a function of strain, two distinct domains, both showing linear relationships, were observed for which an average initial shift rate with respect to strain of -0.63 ± 0.2 cm(-1)%(-1) was observed, followed by an average shift rate of -0.25 ± 0.03 cm(-1)%(-1).
25928444	9	84	theme	GPa	1509:1511	arg1	rate					1487:1490	a Raman band shift rate	1468:1490	a Raman band shift rate of -27 ± 3 cm(-1)GPa(-1)	1468:1515	As a function of stress, a linear relationship was observed with a Raman band shift rate of -27 ± 3 cm(-1)GPa(-1).
25928444	4	85	theme	%	585:585	arg1	fraction					587:594	a 10 wt % fraction	577:594	a 10 wt % fraction of BC	577:600	By adding a 10 wt % fraction of BC in gelatin, visible (λ = 550 nm) and UV (λ = 350 nm) transmittances were found to decrease by ∼35 and 40%, respectively.
25928444	8	86	theme	average	1184:1190	arg1	value					1222:1226	The average initial Raman band shift rate value	1180:1226	The average initial Raman band shift rate value	1180:1226	The average initial Raman band shift rate value corresponds to an average effective Young's modulus of 39 ± 13 GPa and 73 ± 25 GPa, respectively, for either a 2D and 3D network of BC fibrils embedded in the gelatin matrix.
25928444	0	87	theme	Natural	49:55	arg1	Composites					57:66	Gelatin-Matrix Natural Composites	34:66	Gelatin-Matrix Natural Composites with Tunable Optical Properties	34:98	Stress Transfer Quantification in Gelatin-Matrix Natural Composites with Tunable Optical Properties.
25928444	8	88	theme	2D	1339:1340	arg1	network					1349:1355	either a 2D and 3D network	1330:1355	network	1349:1355	The average initial Raman band shift rate value corresponds to an average effective Young's modulus of 39 ± 13 GPa and 73 ± 25 GPa, respectively, for either a 2D and 3D network of BC fibrils embedded in the gelatin matrix.
25928444	10	89	theme	potential	1522:1530	arg1	use					1532:1534	The potential use	1518:1534	The potential use of these composite materials as a UV blocking food coating	1518:1593	The potential use of these composite materials as a UV blocking food coating is discussed.
25928444	8	90	theme	Raman	1200:1204	arg1	value					1222:1226	The average initial Raman band shift rate value	1180:1226	The average initial Raman band shift rate value	1180:1226	The average initial Raman band shift rate value corresponds to an average effective Young's modulus of 39 ± 13 GPa and 73 ± 25 GPa, respectively, for either a 2D and 3D network of BC fibrils embedded in the gelatin matrix.
25928444	3	91	theme	visible	496:502	arg1	light					504:508	visible light	496:508	visible light	496:508	The composite materials showed controllable degrees of transparency to visible light and opacity to UV light depending on BC weight fraction.
25928444	7	92	theme	shift	1140:1144	arg1	rate					1146:1149	an average shift rate	1129:1149	an average shift rate of -0.25 ± 0.03 cm(-1)%(-1)	1129:1177	As a function of strain, two distinct domains, both showing linear relationships, were observed for which an average initial shift rate with respect to strain of -0.63 ± 0.2 cm(-1)%(-1) was observed, followed by an average shift rate of -0.25 ± 0.03 cm(-1)%(-1).
25928444	3	93	theme	composite	429:437	arg1	materials					439:447	The composite materials	425:447	The composite materials	425:447	The composite materials showed controllable degrees of transparency to visible light and opacity to UV light depending on BC weight fraction.
25928444	8	94	theme	3D	1346:1347	arg1	network					1349:1355	either a 2D and 3D network	1330:1355	network	1349:1355	The average initial Raman band shift rate value corresponds to an average effective Young's modulus of 39 ± 13 GPa and 73 ± 25 GPa, respectively, for either a 2D and 3D network of BC fibrils embedded in the gelatin matrix.
25928444	8	95	theme	shift	1211:1215	arg1	value					1222:1226	The average initial Raman band shift rate value	1180:1226	The average initial Raman band shift rate value	1180:1226	The average initial Raman band shift rate value corresponds to an average effective Young's modulus of 39 ± 13 GPa and 73 ± 25 GPa, respectively, for either a 2D and 3D network of BC fibrils embedded in the gelatin matrix.
25928444	8	96	from	matrix	1395:1400	arg1	embedded					1371:1378	embedded	1371:1378	embedded	1371:1378	The average initial Raman band shift rate value corresponds to an average effective Young's modulus of 39 ± 13 GPa and 73 ± 25 GPa, respectively, for either a 2D and 3D network of BC fibrils embedded in the gelatin matrix.
25928444	10	97	theme	composite	1545:1553	arg1	materials					1555:1563	these composite materials	1539:1563	these composite materials	1539:1563	The potential use of these composite materials as a UV blocking food coating is discussed.
25928444	3	98	theme	weight	550:555	arg1	fraction					557:564	BC weight fraction	547:564	BC weight fraction	547:564	The composite materials showed controllable degrees of transparency to visible light and opacity to UV light depending on BC weight fraction.
25928444	8	99	theme	fibrils	1363:1369	arg1	network					1349:1355	either a 2D and 3D network	1330:1355	network	1349:1355	The average initial Raman band shift rate value corresponds to an average effective Young's modulus of 39 ± 13 GPa and 73 ± 25 GPa, respectively, for either a 2D and 3D network of BC fibrils embedded in the gelatin matrix.
25928444	10	100	theme	food	1582:1585	arg1	coating					1587:1593	a UV blocking food coating	1568:1593	a UV blocking food coating	1568:1593	The potential use of these composite materials as a UV blocking food coating is discussed.
25928444	4	101	theme	visible	614:620	arg1	transmittances					655:668	visible (λ = 550 nm) and UV (λ = 350 nm) transmittances	614:668	visible (λ = 550 nm) and UV (λ = 350 nm) transmittances	614:668	By adding a 10 wt % fraction of BC in gelatin, visible (λ = 550 nm) and UV (λ = 350 nm) transmittances were found to decrease by ∼35 and 40%, respectively.
25928444	0	102	theme	Transfer	7:14	arg1	Quantification					16:29	Stress Transfer Quantification	0:29	Stress Transfer Quantification in Gelatin-Matrix Natural Composites with Tunable Optical Properties.	0:99	Stress Transfer Quantification in Gelatin-Matrix Natural Composites with Tunable Optical Properties.
27845783	1	0	from	samples	399:405	arg1	enrichment					351:360	targeted enrichment	342:360	targeted enrichment of glycosylated species from complex samples	342:405	Determining the affinity between a lectin and its target glycans is an important goal, both for understanding the biological functions of a given lectin as well as enabling the use of that lectin for targeted enrichment of glycosylated species from complex samples.
27845783	1	0	from	samples	399:405	arg1	species					378:384	glycosylated species	365:384	glycosylated species from complex samples	365:405	Determining the affinity between a lectin and its target glycans is an important goal, both for understanding the biological functions of a given lectin as well as enabling the use of that lectin for targeted enrichment of glycosylated species from complex samples.
27845783	2	1	theme	such	481:484	arg1	studies					486:492	such studies	481:492	such studies	481:492	While the overall selectivities of many lectins have been characterized, such studies generally require individually purified lectins and glycans.
27845783	4	2	dep	range	730:734	arg1	encounter					766:774	encounter	766:774	encounter	766:774	Rather, lectins display a continuum of affinities for the range of glycan structures they may encounter.
27845783	6	3	from	mixtures	1162:1169	arg1	glycoconjugates					1133:1147	glycoconjugates	1133:1147	glycoconjugates	1133:1147	Here we describe the development of glycan affinity chromatography coupled directly to electrospray mass spectrometry, which enables direct analysis of interactions of lectins with both glycans and glycoconjugates from complex mixtures.
27845783	6	3	from	mixtures	1162:1169	arg1	glycans					1121:1127	glycans	1121:1127	glycans	1121:1127	Here we describe the development of glycan affinity chromatography coupled directly to electrospray mass spectrometry, which enables direct analysis of interactions of lectins with both glycans and glycoconjugates from complex mixtures.
27845783	8	4	theme	constants	1401:1409	arg1	assessment					1378:1387	the direct assessment	1367:1387	the direct assessment of affinity constants between an individual lectin and a large number of glycans in a single experiment, which can be conducted using a complex mixture of unpurified glycans of varying concentrations	1367:1587	Furthermore, this approach allows for the direct assessment of affinity constants between an individual lectin and a large number of glycans in a single experiment, which can be conducted using a complex mixture of unpurified glycans of varying concentrations.
27845783	7	5	theme	elution	1189:1195	arg1	behavior					1197:1204	the elution behavior	1185:1204	the elution behavior of individual species	1185:1226	By observing the elution behavior of individual species, we are able to determine exactly which set of glycoconjugates would be enriched for a given lectin.
27845783	3	6	theme	same	657:660	arg1	affinity					662:669	the same affinity	653:669	the same affinity	653:669	From these analyses, it is clear that a given lectin does not bind all of its target glycans with the same affinity.
27845783	8	7	theme	large	1446:1450	arg1	number					1452:1457	a large number	1444:1457	a large number of glycans in a single experiment	1444:1491	Furthermore, this approach allows for the direct assessment of affinity constants between an individual lectin and a large number of glycans in a single experiment, which can be conducted using a complex mixture of unpurified glycans of varying concentrations.
27845783	4	8	theme	structures	746:755	arg1	range					730:734	the range	726:734	the range of glycan structures they may encounter	726:774	Rather, lectins display a continuum of affinities for the range of glycan structures they may encounter.
27845783	8	9	theme	glycans	1462:1468	arg1	number					1452:1457	a large number	1444:1457	a large number of glycans in a single experiment	1444:1491	Furthermore, this approach allows for the direct assessment of affinity constants between an individual lectin and a large number of glycans in a single experiment, which can be conducted using a complex mixture of unpurified glycans of varying concentrations.
27845783	8	9	theme	glycans	1462:1468	arg1	lectin					1433:1438	an individual lectin	1419:1438	an individual lectin	1419:1438	Furthermore, this approach allows for the direct assessment of affinity constants between an individual lectin and a large number of glycans in a single experiment, which can be conducted using a complex mixture of unpurified glycans of varying concentrations.
27845783	1	10	theme	biological	256:265	arg1	functions					267:275	the biological functions	252:275	the biological functions of a given lectin	252:293	Determining the affinity between a lectin and its target glycans is an important goal, both for understanding the biological functions of a given lectin as well as enabling the use of that lectin for targeted enrichment of glycosylated species from complex samples.
27845783	1	11	theme	lectin	331:336	arg1	use					319:321	the use	315:321	the use of that lectin for targeted enrichment of glycosylated species from complex samples	315:405	Determining the affinity between a lectin and its target glycans is an important goal, both for understanding the biological functions of a given lectin as well as enabling the use of that lectin for targeted enrichment of glycosylated species from complex samples.
27845783	6	12	theme	complex	1154:1160	arg1	mixtures					1162:1169	complex mixtures	1154:1169	complex mixtures	1154:1169	Here we describe the development of glycan affinity chromatography coupled directly to electrospray mass spectrometry, which enables direct analysis of interactions of lectins with both glycans and glycoconjugates from complex mixtures.
27845783	6	13	theme	lectins	1103:1109	arg1	interactions					1087:1098	interactions	1087:1098	interactions of lectins with both glycans and glycoconjugates from complex mixtures	1087:1169	Here we describe the development of glycan affinity chromatography coupled directly to electrospray mass spectrometry, which enables direct analysis of interactions of lectins with both glycans and glycoconjugates from complex mixtures.
27845783	0	14	theme	glycopeptide	91:102	arg1	enrichment					104:113	glycopeptide enrichment	91:113	glycopeptide enrichment	91:113	Characterization of lectin binding affinities via direct LC-MS profiling: implications for glycopeptide enrichment and separation strategies.
27845783	8	15	theme	single	1475:1480	arg1	experiment					1482:1491	a single experiment	1473:1491	a single experiment	1473:1491	Furthermore, this approach allows for the direct assessment of affinity constants between an individual lectin and a large number of glycans in a single experiment, which can be conducted using a complex mixture of unpurified glycans of varying concentrations.
27845783	7	16	theme	species	1220:1226	arg1	behavior					1197:1204	the elution behavior	1185:1204	the elution behavior of individual species	1185:1226	By observing the elution behavior of individual species, we are able to determine exactly which set of glycoconjugates would be enriched for a given lectin.
27845783	7	17	theme	individual	1209:1218	arg1	species					1220:1226	individual species	1209:1226	individual species	1209:1226	By observing the elution behavior of individual species, we are able to determine exactly which set of glycoconjugates would be enriched for a given lectin.
27845783	0	18	theme	separation	119:128	arg1	strategies					130:139	separation strategies	119:139	separation strategies	119:139	Characterization of lectin binding affinities via direct LC-MS profiling: implications for glycopeptide enrichment and separation strategies.
27845783	1	19	theme	targeted	342:349	arg1	enrichment					351:360	targeted enrichment	342:360	targeted enrichment of glycosylated species from complex samples	342:405	Determining the affinity between a lectin and its target glycans is an important goal, both for understanding the biological functions of a given lectin as well as enabling the use of that lectin for targeted enrichment of glycosylated species from complex samples.
27845783	4	20	theme	glycan	739:744	arg1	structures					746:755	glycan structures	739:755	glycan structures	739:755	Rather, lectins display a continuum of affinities for the range of glycan structures they may encounter.
27845783	3	21	with	glycans	640:646	arg1	affinity					662:669	the same affinity	653:669	the same affinity	653:669	From these analyses, it is clear that a given lectin does not bind all of its target glycans with the same affinity.
27845783	2	22	theme	lectins	448:454	arg1	selectivities					426:438	the overall selectivities	414:438	the overall selectivities of many lectins	414:454	While the overall selectivities of many lectins have been characterized, such studies generally require individually purified lectins and glycans.
27845783	8	23	from	experiment	1482:1491	arg1	number					1452:1457	a large number	1444:1457	a large number of glycans in a single experiment	1444:1491	Furthermore, this approach allows for the direct assessment of affinity constants between an individual lectin and a large number of glycans in a single experiment, which can be conducted using a complex mixture of unpurified glycans of varying concentrations.
27845783	8	23	from	experiment	1482:1491	arg1	lectin					1433:1438	an individual lectin	1419:1438	an individual lectin	1419:1438	Furthermore, this approach allows for the direct assessment of affinity constants between an individual lectin and a large number of glycans in a single experiment, which can be conducted using a complex mixture of unpurified glycans of varying concentrations.
27845783	8	24	theme	concentrations	1574:1587	arg1	glycans					1555:1561	unpurified glycans	1544:1561	unpurified glycans of varying concentrations	1544:1587	Furthermore, this approach allows for the direct assessment of affinity constants between an individual lectin and a large number of glycans in a single experiment, which can be conducted using a complex mixture of unpurified glycans of varying concentrations.
27845783	6	25	theme	mass	1035:1038	arg1	spectrometry					1040:1051	electrospray mass spectrometry	1022:1051	electrospray mass spectrometry	1022:1051	Here we describe the development of glycan affinity chromatography coupled directly to electrospray mass spectrometry, which enables direct analysis of interactions of lectins with both glycans and glycoconjugates from complex mixtures.
27845783	1	26	from	enrichment	351:360	arg1	samples					399:405	complex samples	391:405	complex samples	391:405	Determining the affinity between a lectin and its target glycans is an important goal, both for understanding the biological functions of a given lectin as well as enabling the use of that lectin for targeted enrichment of glycosylated species from complex samples.
27845783	8	27	theme	direct	1371:1376	arg1	assessment					1378:1387	the direct assessment	1367:1387	the direct assessment of affinity constants between an individual lectin and a large number of glycans in a single experiment, which can be conducted using a complex mixture of unpurified glycans of varying concentrations	1367:1587	Furthermore, this approach allows for the direct assessment of affinity constants between an individual lectin and a large number of glycans in a single experiment, which can be conducted using a complex mixture of unpurified glycans of varying concentrations.
27845783	0	28	gly	glycopeptide	91:102	arg2	glycopeptide					91:102	glycopeptide enrichment	91:113	glycopeptide enrichment	91:113	Characterization of lectin binding affinities via direct LC-MS profiling: implications for glycopeptide enrichment and separation strategies.
27845783	6	29	theme	electrospray	1022:1033	arg1	spectrometry					1040:1051	electrospray mass spectrometry	1022:1051	electrospray mass spectrometry	1022:1051	Here we describe the development of glycan affinity chromatography coupled directly to electrospray mass spectrometry, which enables direct analysis of interactions of lectins with both glycans and glycoconjugates from complex mixtures.
27845783	1	30	theme	target	192:197	arg1	glycans					199:205	its target glycans	188:205	its target glycans	188:205	Determining the affinity between a lectin and its target glycans is an important goal, both for understanding the biological functions of a given lectin as well as enabling the use of that lectin for targeted enrichment of glycosylated species from complex samples.
27845783	8	31	from	glycans	1462:1468	arg1	experiment					1482:1491	a single experiment	1473:1491	a single experiment	1473:1491	Furthermore, this approach allows for the direct assessment of affinity constants between an individual lectin and a large number of glycans in a single experiment, which can be conducted using a complex mixture of unpurified glycans of varying concentrations.
27845783	1	32	dep	understanding	238:250	arg1	both					229:232	both	229:232	both	229:232	Determining the affinity between a lectin and its target glycans is an important goal, both for understanding the biological functions of a given lectin as well as enabling the use of that lectin for targeted enrichment of glycosylated species from complex samples.
27845783	0	33	theme	binding	27:33	arg1	affinities					35:44	lectin binding affinities	20:44	lectin binding affinities	20:44	Characterization of lectin binding affinities via direct LC-MS profiling: implications for glycopeptide enrichment and separation strategies.
27845783	1	34	theme	given	282:286	arg1	lectin					288:293	a given lectin	280:293	a given lectin	280:293	Determining the affinity between a lectin and its target glycans is an important goal, both for understanding the biological functions of a given lectin as well as enabling the use of that lectin for targeted enrichment of glycosylated species from complex samples.
27845783	8	35	from	number	1452:1457	arg1	experiment					1482:1491	a single experiment	1473:1491	a single experiment	1473:1491	Furthermore, this approach allows for the direct assessment of affinity constants between an individual lectin and a large number of glycans in a single experiment, which can be conducted using a complex mixture of unpurified glycans of varying concentrations.
27845783	6	36	theme	chromatography	987:1000	arg1	development					956:966	the development	952:966	the development of glycan affinity chromatography coupled directly to electrospray mass spectrometry, which enables direct analysis of interactions of lectins with both glycans and glycoconjugates from complex mixtures	952:1169	Here we describe the development of glycan affinity chromatography coupled directly to electrospray mass spectrometry, which enables direct analysis of interactions of lectins with both glycans and glycoconjugates from complex mixtures.
27845783	8	37	from	lectin	1433:1438	arg1	experiment					1482:1491	a single experiment	1473:1491	a single experiment	1473:1491	Furthermore, this approach allows for the direct assessment of affinity constants between an individual lectin and a large number of glycans in a single experiment, which can be conducted using a complex mixture of unpurified glycans of varying concentrations.
27845783	0	38	theme	lectin	20:25	arg1	affinities					35:44	lectin binding affinities	20:44	lectin binding affinities	20:44	Characterization of lectin binding affinities via direct LC-MS profiling: implications for glycopeptide enrichment and separation strategies.
27845783	1	39	theme	lectin	288:293	arg1	functions					267:275	the biological functions	252:275	the biological functions of a given lectin	252:293	Determining the affinity between a lectin and its target glycans is an important goal, both for understanding the biological functions of a given lectin as well as enabling the use of that lectin for targeted enrichment of glycosylated species from complex samples.
27845783	2	40	dep	require	504:510	arg1	purified					525:532	purified	525:532	require individually purified lectins and glycans	504:552	While the overall selectivities of many lectins have been characterized, such studies generally require individually purified lectins and glycans.
27845783	1	41	theme	glycosylated	365:376	arg1	species					378:384	glycosylated species	365:384	glycosylated species from complex samples	365:405	Determining the affinity between a lectin and its target glycans is an important goal, both for understanding the biological functions of a given lectin as well as enabling the use of that lectin for targeted enrichment of glycosylated species from complex samples.
27845783	4	42	theme	affinities	711:720	arg1	continuum					698:706	a continuum	696:706	a continuum of affinities for the range of glycan structures they may encounter	696:774	Rather, lectins display a continuum of affinities for the range of glycan structures they may encounter.
27845783	8	43	theme	affinity	1392:1399	arg1	constants					1401:1409	affinity constants	1392:1409	affinity constants between an individual lectin and a large number of glycans in a single experiment, which can be conducted using a complex mixture of unpurified glycans of varying concentrations	1392:1587	Furthermore, this approach allows for the direct assessment of affinity constants between an individual lectin and a large number of glycans in a single experiment, which can be conducted using a complex mixture of unpurified glycans of varying concentrations.
27845783	6	44	theme	interactions	1087:1098	arg1	analysis					1075:1082	direct analysis	1068:1082	direct analysis of interactions of lectins with both glycans and glycoconjugates from complex mixtures	1068:1169	Here we describe the development of glycan affinity chromatography coupled directly to electrospray mass spectrometry, which enables direct analysis of interactions of lectins with both glycans and glycoconjugates from complex mixtures.
27845783	1	45	theme	species	378:384	arg1	enrichment					351:360	targeted enrichment	342:360	targeted enrichment of glycosylated species from complex samples	342:405	Determining the affinity between a lectin and its target glycans is an important goal, both for understanding the biological functions of a given lectin as well as enabling the use of that lectin for targeted enrichment of glycosylated species from complex samples.
27845783	1	46	theme	important	213:221	arg1	goal					223:226	an important goal	210:226	an important goal	210:226	Determining the affinity between a lectin and its target glycans is an important goal, both for understanding the biological functions of a given lectin as well as enabling the use of that lectin for targeted enrichment of glycosylated species from complex samples.
27845783	0	47	theme	affinities	35:44	arg1	Characterization					0:15	Characterization	0:15	Characterization of lectin binding affinities via direct LC-MS profiling: implications for glycopeptide enrichment and separation strategies.	0:140	Characterization of lectin binding affinities via direct LC-MS profiling: implications for glycopeptide enrichment and separation strategies.
27845783	8	48	theme	varying	1566:1572	arg1	concentrations					1574:1587	varying concentrations	1566:1587	varying concentrations	1566:1587	Furthermore, this approach allows for the direct assessment of affinity constants between an individual lectin and a large number of glycans in a single experiment, which can be conducted using a complex mixture of unpurified glycans of varying concentrations.
27845783	8	49	theme	individual	1422:1431	arg1	lectin					1433:1438	an individual lectin	1419:1438	an individual lectin	1419:1438	Furthermore, this approach allows for the direct assessment of affinity constants between an individual lectin and a large number of glycans in a single experiment, which can be conducted using a complex mixture of unpurified glycans of varying concentrations.
27845783	6	50	theme	affinity	978:985	arg1	chromatography					987:1000	glycan affinity chromatography	971:1000	glycan affinity chromatography coupled directly to electrospray mass spectrometry, which enables direct analysis of interactions of lectins with both glycans and glycoconjugates from complex mixtures	971:1169	Here we describe the development of glycan affinity chromatography coupled directly to electrospray mass spectrometry, which enables direct analysis of interactions of lectins with both glycans and glycoconjugates from complex mixtures.
27845783	0	51	theme	LC-MS	57:61	arg1	profiling					63:71	direct LC-MS profiling	50:71	direct LC-MS profiling	50:71	Characterization of lectin binding affinities via direct LC-MS profiling: implications for glycopeptide enrichment and separation strategies.
27845783	6	52	with	lectins	1103:1109	arg1	glycoconjugates					1133:1147	glycoconjugates	1133:1147	glycoconjugates	1133:1147	Here we describe the development of glycan affinity chromatography coupled directly to electrospray mass spectrometry, which enables direct analysis of interactions of lectins with both glycans and glycoconjugates from complex mixtures.
27845783	6	52	with	lectins	1103:1109	arg1	glycans					1121:1127	glycans	1121:1127	glycans	1121:1127	Here we describe the development of glycan affinity chromatography coupled directly to electrospray mass spectrometry, which enables direct analysis of interactions of lectins with both glycans and glycoconjugates from complex mixtures.
27845783	1	53	theme	complex	391:397	arg1	samples					399:405	complex samples	391:405	complex samples	391:405	Determining the affinity between a lectin and its target glycans is an important goal, both for understanding the biological functions of a given lectin as well as enabling the use of that lectin for targeted enrichment of glycosylated species from complex samples.
27845783	0	54	theme	direct	50:55	arg1	profiling					63:71	direct LC-MS profiling	50:71	direct LC-MS profiling	50:71	Characterization of lectin binding affinities via direct LC-MS profiling: implications for glycopeptide enrichment and separation strategies.
27845783	5	55	theme	structures	868:877	arg1	structures					868:877	structures	868:877	structures	868:877	Because of this continuum, it is not straightforward in practice to determine which set of structures will be enriched using a lectin as an affinity reagent.
27845783	5	55	theme	structures	868:877	arg1	set					861:863	set	861:863	set of structures	861:877	Because of this continuum, it is not straightforward in practice to determine which set of structures will be enriched using a lectin as an affinity reagent.
27845783	8	56	theme	complex	1525:1531	arg1	mixture					1533:1539	a complex mixture	1523:1539	a complex mixture of unpurified glycans of varying concentrations	1523:1587	Furthermore, this approach allows for the direct assessment of affinity constants between an individual lectin and a large number of glycans in a single experiment, which can be conducted using a complex mixture of unpurified glycans of varying concentrations.
27845783	7	57	theme	given	1315:1319	arg1	lectin					1321:1326	a given lectin	1313:1326	a given lectin	1313:1326	By observing the elution behavior of individual species, we are able to determine exactly which set of glycoconjugates would be enriched for a given lectin.
27845783	1	58	gly	glycosylated	365:376	arg1	species					378:384	glycosylated species	365:384	glycosylated species from complex samples	365:405	Determining the affinity between a lectin and its target glycans is an important goal, both for understanding the biological functions of a given lectin as well as enabling the use of that lectin for targeted enrichment of glycosylated species from complex samples.
27845783	6	59	theme	glycan	971:976	arg1	chromatography					987:1000	glycan affinity chromatography	971:1000	glycan affinity chromatography coupled directly to electrospray mass spectrometry, which enables direct analysis of interactions of lectins with both glycans and glycoconjugates from complex mixtures	971:1169	Here we describe the development of glycan affinity chromatography coupled directly to electrospray mass spectrometry, which enables direct analysis of interactions of lectins with both glycans and glycoconjugates from complex mixtures.
27845783	3	60	theme	target	633:638	arg1	glycans					640:646	its target glycans	629:646	its target glycans with the same affinity	629:669	From these analyses, it is clear that a given lectin does not bind all of its target glycans with the same affinity.
27845783	1	61	dep	goal	223:226	arg1	understanding					238:250	understanding	238:250	understanding the biological functions of a given lectin as well as enabling the use of that lectin for targeted enrichment of glycosylated species from complex samples	238:405	Determining the affinity between a lectin and its target glycans is an important goal, both for understanding the biological functions of a given lectin as well as enabling the use of that lectin for targeted enrichment of glycosylated species from complex samples.
27845783	1	61	dep	goal	223:226	arg1	enabling					306:313	enabling	306:313	understanding the biological functions of a given lectin as well as enabling the use of that lectin for targeted enrichment of glycosylated species from complex samples	238:405	Determining the affinity between a lectin and its target glycans is an important goal, both for understanding the biological functions of a given lectin as well as enabling the use of that lectin for targeted enrichment of glycosylated species from complex samples.
27845783	7	62	theme	glycoconjugates	1275:1289	arg1	set					1268:1270	set	1268:1270	set of glycoconjugates	1268:1289	By observing the elution behavior of individual species, we are able to determine exactly which set of glycoconjugates would be enriched for a given lectin.
27845783	7	62	theme	glycoconjugates	1275:1289	arg1	glycoconjugates					1275:1289	glycoconjugates	1275:1289	glycoconjugates	1275:1289	By observing the elution behavior of individual species, we are able to determine exactly which set of glycoconjugates would be enriched for a given lectin.
27845783	8	63	theme	unpurified	1544:1553	arg1	glycans					1555:1561	unpurified glycans	1544:1561	unpurified glycans of varying concentrations	1544:1587	Furthermore, this approach allows for the direct assessment of affinity constants between an individual lectin and a large number of glycans in a single experiment, which can be conducted using a complex mixture of unpurified glycans of varying concentrations.
27845783	5	64	theme	affinity	917:924	arg1	which					855:859	which	855:859	which	855:859	Because of this continuum, it is not straightforward in practice to determine which set of structures will be enriched using a lectin as an affinity reagent.
27845783	5	64	theme	affinity	917:924	arg1	lectin					904:909	a lectin	902:909	a lectin	902:909	Because of this continuum, it is not straightforward in practice to determine which set of structures will be enriched using a lectin as an affinity reagent.
27845783	5	64	theme	affinity	917:924	arg1	reagent					926:932	an affinity reagent	914:932	an affinity reagent	914:932	Because of this continuum, it is not straightforward in practice to determine which set of structures will be enriched using a lectin as an affinity reagent.
27845783	3	65	theme	given	595:599	arg1	lectin					601:606	a given lectin	593:606	a given lectin	593:606	From these analyses, it is clear that a given lectin does not bind all of its target glycans with the same affinity.
27845783	2	66	theme	overall	418:424	arg1	selectivities					426:438	the overall selectivities	414:438	the overall selectivities of many lectins	414:454	While the overall selectivities of many lectins have been characterized, such studies generally require individually purified lectins and glycans.
27845783	6	67	theme	direct	1068:1073	arg1	analysis					1075:1082	direct analysis	1068:1082	direct analysis of interactions of lectins with both glycans and glycoconjugates from complex mixtures	1068:1169	Here we describe the development of glycan affinity chromatography coupled directly to electrospray mass spectrometry, which enables direct analysis of interactions of lectins with both glycans and glycoconjugates from complex mixtures.
27845783	3	68	from	analyses	566:573	arg1	clear					582:586	clear	582:586	clear	582:586	From these analyses, it is clear that a given lectin does not bind all of its target glycans with the same affinity.
27845783	0	69	dep	Characterization	0:15	arg1	implications					74:85	implications	74:85	Characterization of lectin binding affinities via direct LC-MS profiling: implications for glycopeptide enrichment and separation strategies.	0:140	Characterization of lectin binding affinities via direct LC-MS profiling: implications for glycopeptide enrichment and separation strategies.
27845783	2	70	theme	many	443:446	arg1	lectins					448:454	many lectins	443:454	many lectins	443:454	While the overall selectivities of many lectins have been characterized, such studies generally require individually purified lectins and glycans.
27845783	8	71	theme	glycans	1555:1561	arg1	mixture					1533:1539	a complex mixture	1523:1539	a complex mixture of unpurified glycans of varying concentrations	1523:1587	Furthermore, this approach allows for the direct assessment of affinity constants between an individual lectin and a large number of glycans in a single experiment, which can be conducted using a complex mixture of unpurified glycans of varying concentrations.
29291859	0	0	theme	dairy	98:102	arg1	technology					104:113	dairy technology	98:113	dairy technology	98:113	Highly stable novel silica/chitosan support for β-galactosidase immobilization for application in dairy technology.
29291859	5	1	theme	biocatalysts	648:659	arg1	Performance					629:639	Performance	629:639	Performance of the biocatalysts	629:659	Performance of the biocatalysts was tested for lactose hydrolysis, and the enzyme immobilized in SiQT10 and SiQT20 composites showed higher efficiency (62% and 47%, respectively) compared with the enzyme in SiO2aptms.
29291859	8	2	theme	food	1074:1077	arg1	processing					1079:1088	food processing	1074:1088	food processing	1074:1088	Thus, in addition to demonstrating compatibility for food processing, these results align the enzyme stabilization properties of chitosan with the mechanical resistance of silica.
29291859	7	3	theme	continuous	933:942	arg1	use					944:946	continuous use	933:946	continuous use	933:946	After 200 h of continuous use in a fixed-bed reactor, SiQT10 remained with approximately 90% activity.
29291859	8	4	theme	stabilization	1122:1134	arg1	properties					1136:1145	the enzyme stabilization properties	1111:1145	the enzyme stabilization properties of chitosan	1111:1157	Thus, in addition to demonstrating compatibility for food processing, these results align the enzyme stabilization properties of chitosan with the mechanical resistance of silica.
29291859	7	5	theme	use	944:946	arg1	200 h					924:928	200 h	924:928	200 h of continuous use in a fixed-bed reactor	924:969	After 200 h of continuous use in a fixed-bed reactor, SiQT10 remained with approximately 90% activity.
29291859	6	6	from	stability	859:867	arg1	system					877:882	this system	872:882	this system	872:882	Operational stability in this system was evaluated for the first time.
29291859	0	7	from	immobilization	64:77	arg1	technology					104:113	dairy technology	98:113	dairy technology	98:113	Highly stable novel silica/chitosan support for β-galactosidase immobilization for application in dairy technology.
29291859	7	8	dep	%	1009:1009	arg1	90					1007:1008	90	1007:1008	90	1007:1008	After 200 h of continuous use in a fixed-bed reactor, SiQT10 remained with approximately 90% activity.
29291859	3	9	from	regions	503:509	arg1	silica					514:519	silica	514:519	silica	514:519	Morphological and textural characterizations showed that chitosan is dispersed in micrometric regions in silica.
29291859	2	10	theme	chitosan	365:372	arg1	%					363:363	20% chitosan	361:372	20% chitosan (SiQT10 and SiQT20, respectively)	361:406	Here, we report immobilization of the enzyme on a silica/chitosan composite support, devised to have 10% and 20% chitosan (SiQT10 and SiQT20, respectively).
29291859	7	11	dep	activity	1011:1018	arg1	%					1009:1009	%	1009:1009	%	1009:1009	After 200 h of continuous use in a fixed-bed reactor, SiQT10 remained with approximately 90% activity.
29291859	1	12	from	enzyme	150:155	arg1	industry					170:177	the dairy industry	160:177	the dairy industry	160:177	β-d-Galactosidase is an important enzyme in the dairy industry, and the enzyme from the yeast Kluyveromyces lactis is most widely used.
29291859	0	13	theme	stable	7:12	arg1	silica/chitosan					20:34	Highly stable novel silica/chitosan	0:34	Highly stable novel silica/chitosan	0:34	Highly stable novel silica/chitosan support for β-galactosidase immobilization for application in dairy technology.
29291859	2	14	theme	silica/chitosan	302:316	arg1	support					328:334	a silica/chitosan composite support	300:334	a silica/chitosan composite support	300:334	Here, we report immobilization of the enzyme on a silica/chitosan composite support, devised to have 10% and 20% chitosan (SiQT10 and SiQT20, respectively).
29291859	8	15	theme	chitosan	1150:1157	arg1	properties					1136:1145	the enzyme stabilization properties	1111:1145	the enzyme stabilization properties of chitosan	1111:1157	Thus, in addition to demonstrating compatibility for food processing, these results align the enzyme stabilization properties of chitosan with the mechanical resistance of silica.
29291859	8	16	theme	silica	1193:1198	arg1	resistance					1179:1188	the mechanical resistance	1164:1188	the mechanical resistance of silica	1164:1198	Thus, in addition to demonstrating compatibility for food processing, these results align the enzyme stabilization properties of chitosan with the mechanical resistance of silica.
29291859	5	17	dep	efficiency	769:778	arg1	%					783:783	62%	781:783	62%	781:783	Performance of the biocatalysts was tested for lactose hydrolysis, and the enzyme immobilized in SiQT10 and SiQT20 composites showed higher efficiency (62% and 47%, respectively) compared with the enzyme in SiO2aptms.
29291859	5	17	dep	efficiency	769:778	arg1	%					791:791	47%	789:791	47%	789:791	Performance of the biocatalysts was tested for lactose hydrolysis, and the enzyme immobilized in SiQT10 and SiQT20 composites showed higher efficiency (62% and 47%, respectively) compared with the enzyme in SiO2aptms.
29291859	3	18	theme	textural	427:434	arg1	characterizations					436:452	Morphological and textural characterizations	409:452	Morphological and textural characterizations	409:452	Morphological and textural characterizations showed that chitosan is dispersed in micrometric regions in silica.
29291859	0	19	theme	novel	14:18	arg1	silica/chitosan					20:34	Highly stable novel silica/chitosan	0:34	Highly stable novel silica/chitosan	0:34	Highly stable novel silica/chitosan support for β-galactosidase immobilization for application in dairy technology.
29291859	6	20	theme	Operational	847:857	arg1	stability					859:867	Operational stability	847:867	Operational stability in this system	847:882	Operational stability in this system was evaluated for the first time.
29291859	2	21	theme	enzyme	290:295	arg1	immobilization					268:281	immobilization	268:281	immobilization of the enzyme on a silica/chitosan composite support, devised to have 10% and 20% chitosan (SiQT10 and SiQT20, respectively)	268:406	Here, we report immobilization of the enzyme on a silica/chitosan composite support, devised to have 10% and 20% chitosan (SiQT10 and SiQT20, respectively).
29291859	7	22	from	200 h	924:928	arg1	reactor					963:969	a fixed-bed reactor	951:969	a fixed-bed reactor	951:969	After 200 h of continuous use in a fixed-bed reactor, SiQT10 remained with approximately 90% activity.
29291859	3	23	theme	Morphological	409:421	arg1	characterizations					436:452	Morphological and textural characterizations	409:452	Morphological and textural characterizations	409:452	Morphological and textural characterizations showed that chitosan is dispersed in micrometric regions in silica.
29291859	8	24	dep	demonstrating	1042:1054	arg1	addition					1030:1037	addition	1030:1037	addition	1030:1037	Thus, in addition to demonstrating compatibility for food processing, these results align the enzyme stabilization properties of chitosan with the mechanical resistance of silica.
29291859	8	25	theme	mechanical	1168:1177	arg1	resistance					1179:1188	the mechanical resistance	1164:1188	the mechanical resistance of silica	1164:1198	Thus, in addition to demonstrating compatibility for food processing, these results align the enzyme stabilization properties of chitosan with the mechanical resistance of silica.
29291859	6	26	theme	first	906:910	arg1	time					912:915	the first time	902:915	the first time	902:915	Operational stability in this system was evaluated for the first time.
29291859	5	27	theme	lactose	676:682	arg1	hydrolysis					684:693	lactose hydrolysis	676:693	lactose hydrolysis	676:693	Performance of the biocatalysts was tested for lactose hydrolysis, and the enzyme immobilized in SiQT10 and SiQT20 composites showed higher efficiency (62% and 47%, respectively) compared with the enzyme in SiO2aptms.
29291859	5	28	from	enzyme	826:831	arg1	SiO2aptms					836:844	SiO2aptms	836:844	SiO2aptms	836:844	Performance of the biocatalysts was tested for lactose hydrolysis, and the enzyme immobilized in SiQT10 and SiQT20 composites showed higher efficiency (62% and 47%, respectively) compared with the enzyme in SiO2aptms.
29291859	0	29	theme	β-galactosidase	48:62	arg1	immobilization					64:77	β-galactosidase immobilization	48:77	β-galactosidase immobilization for application in dairy technology	48:113	Highly stable novel silica/chitosan support for β-galactosidase immobilization for application in dairy technology.
29291859	1	30	used	used	246:249	arg2	enzyme					188:193	the enzyme	184:193	the enzyme from the yeast Kluyveromyces lactis	184:229	β-d-Galactosidase is an important enzyme in the dairy industry, and the enzyme from the yeast Kluyveromyces lactis is most widely used.
29291859	1	31	theme	important	140:148	arg1	enzyme					150:155	an important enzyme	137:155	an important enzyme in the dairy industry	137:177	β-d-Galactosidase is an important enzyme in the dairy industry, and the enzyme from the yeast Kluyveromyces lactis is most widely used.
29291859	1	31	theme	important	140:148	arg1	β-d-Galactosidase					116:132	β-d-Galactosidase	116:132	β-d-Galactosidase	116:132	β-d-Galactosidase is an important enzyme in the dairy industry, and the enzyme from the yeast Kluyveromyces lactis is most widely used.
29291859	2	32	from	immobilization	268:281	arg1	support					328:334	a silica/chitosan composite support	300:334	a silica/chitosan composite support	300:334	Here, we report immobilization of the enzyme on a silica/chitosan composite support, devised to have 10% and 20% chitosan (SiQT10 and SiQT20, respectively).
29291859	2	33	dep	%	363:363	arg1	SiQT10					375:380	SiQT10	375:380	SiQT10	375:380	Here, we report immobilization of the enzyme on a silica/chitosan composite support, devised to have 10% and 20% chitosan (SiQT10 and SiQT20, respectively).
29291859	2	33	dep	%	363:363	arg1	SiQT20					386:391	SiQT20	386:391	SiQT20	386:391	Here, we report immobilization of the enzyme on a silica/chitosan composite support, devised to have 10% and 20% chitosan (SiQT10 and SiQT20, respectively).
29291859	5	34	dep	SiQT10	726:731	arg1	composites					744:753	composites	744:753	composites	744:753	Performance of the biocatalysts was tested for lactose hydrolysis, and the enzyme immobilized in SiQT10 and SiQT20 composites showed higher efficiency (62% and 47%, respectively) compared with the enzyme in SiO2aptms.
29291859	1	35	from	yeast	204:208	arg1	enzyme					188:193	the enzyme	184:193	the enzyme from the yeast Kluyveromyces lactis	184:229	β-d-Galactosidase is an important enzyme in the dairy industry, and the enzyme from the yeast Kluyveromyces lactis is most widely used.
29291859	7	36	theme	fixed-bed	953:961	arg1	reactor					963:969	a fixed-bed reactor	951:969	a fixed-bed reactor	951:969	After 200 h of continuous use in a fixed-bed reactor, SiQT10 remained with approximately 90% activity.
29291859	2	37	theme	composite	318:326	arg1	support					328:334	a silica/chitosan composite support	300:334	a silica/chitosan composite support	300:334	Here, we report immobilization of the enzyme on a silica/chitosan composite support, devised to have 10% and 20% chitosan (SiQT10 and SiQT20, respectively).
29291859	3	38	theme	micrometric	491:501	arg1	regions					503:509	micrometric regions	491:509	micrometric regions in silica	491:519	Morphological and textural characterizations showed that chitosan is dispersed in micrometric regions in silica.
29291859	5	39	theme	higher	762:767	arg1	efficiency					769:778	higher efficiency	762:778	higher efficiency (62% and 47%, respectively)	762:806	Performance of the biocatalysts was tested for lactose hydrolysis, and the enzyme immobilized in SiQT10 and SiQT20 composites showed higher efficiency (62% and 47%, respectively) compared with the enzyme in SiO2aptms.
29291859	1	40	dep	yeast	204:208	arg1	lactis					224:229	Kluyveromyces lactis	210:229	the yeast Kluyveromyces lactis	200:229	β-d-Galactosidase is an important enzyme in the dairy industry, and the enzyme from the yeast Kluyveromyces lactis is most widely used.
29291859	1	41	theme	dairy	164:168	arg1	industry					170:177	the dairy industry	160:177	the dairy industry	160:177	β-d-Galactosidase is an important enzyme in the dairy industry, and the enzyme from the yeast Kluyveromyces lactis is most widely used.
29291859	8	42	theme	enzyme	1115:1120	arg1	properties					1136:1145	the enzyme stabilization properties	1111:1145	the enzyme stabilization properties of chitosan	1111:1157	Thus, in addition to demonstrating compatibility for food processing, these results align the enzyme stabilization properties of chitosan with the mechanical resistance of silica.
28962778	0	0	theme	dye	107:109	arg1	removal					111:117	dye removal	107:117	dye removal	107:117	Chitosan hydrogels embedding hyper-crosslinked polymer particles as reusable broad-spectrum adsorbents for dye removal.
28962778	7	1	theme	pure	1121:1124	arg1	CS					1126:1127	pure CS	1121:1127	pure CS	1121:1127	Moreover, the mechanical properties of the composite hydrogels are enhanced respect to pure CS, and the samples can be regenerated and reused keeping their adsorption ability unaltered over successive cycles of adsorption, desorption, and washing.
28962778	2	2	theme	hyper-crosslinked	373:389	arg1	particles					405:413	hyper-crosslinked polymer (HCP) particles	373:413	hyper-crosslinked polymer (HCP) particles	373:413	To address this challenge, we developed composite chitosan (CS)-based hydrogels containing hyper-crosslinked polymer (HCP) particles to be used as broad-spectrum adsorbents.
28962778	2	3	contain	containing	362:371	arg1	hydrogels					352:360	composite chitosan (CS)-based hydrogels	322:360	composite chitosan (CS)-based hydrogels containing hyper-crosslinked polymer (HCP) particles	322:413	To address this challenge, we developed composite chitosan (CS)-based hydrogels containing hyper-crosslinked polymer (HCP) particles to be used as broad-spectrum adsorbents.
28962778	2	3	contain	containing	362:371	arg2	particles					405:413	hyper-crosslinked polymer (HCP) particles	373:413	hyper-crosslinked polymer (HCP) particles	373:413	To address this challenge, we developed composite chitosan (CS)-based hydrogels containing hyper-crosslinked polymer (HCP) particles to be used as broad-spectrum adsorbents.
28962778	5	4	theme	CS	815:816	arg1	particles					826:834	CS and HCP particles	815:834	CS and HCP particles	815:834	Adsorption experiments reveal a synergistic effect between CS and HCP particles, and the samples are able to remove both anionic and cationic dyes (indigo carmine, rhodamine 6G and sunset yellow) from water.
28962778	0	5	link	hyper-crosslinked	29:45	arg1	particles					55:63	embedding hyper-crosslinked polymer particles	19:63	embedding hyper-crosslinked polymer particles	19:63	Chitosan hydrogels embedding hyper-crosslinked polymer particles as reusable broad-spectrum adsorbents for dye removal.
28962778	0	5	link	hyper-crosslinked	29:45	arg1	adsorbents					92:101	reusable broad-spectrum adsorbents	68:101	reusable broad-spectrum adsorbents for dye removal	68:117	Chitosan hydrogels embedding hyper-crosslinked polymer particles as reusable broad-spectrum adsorbents for dye removal.
28962778	3	6	theme	chitosan	521:528	arg1	capacity					538:545	the capacity	534:545	the capacity of the porous particles of trapping pollutant molecules	534:601	The goal is to efficiently combine the dye adsorption ability of chitosan and the capacity of the porous particles of trapping pollutant molecules.
28962778	3	6	theme	chitosan	521:528	arg1	ability					510:516	the dye adsorption ability	491:516	the dye adsorption ability of chitosan	491:528	The goal is to efficiently combine the dye adsorption ability of chitosan and the capacity of the porous particles of trapping pollutant molecules.
28962778	5	7	theme	Adsorption	756:765	arg1	experiments					767:777	Adsorption experiments	756:777	Adsorption experiments	756:777	Adsorption experiments reveal a synergistic effect between CS and HCP particles, and the samples are able to remove both anionic and cationic dyes (indigo carmine, rhodamine 6G and sunset yellow) from water.
28962778	1	8	from	water	171:175	arg1	issue					203:207	an extremely important issue	180:207	an extremely important issue that requires systematic and efficient adsorbent preparation strategies	180:279	The removal of dye and toxic ionic pollutants from water is an extremely important issue that requires systematic and efficient adsorbent preparation strategies.
28962778	1	8	from	water	171:175	arg1	removal					124:130	The removal	120:130	The removal of dye and toxic ionic pollutants from water	120:175	The removal of dye and toxic ionic pollutants from water is an extremely important issue that requires systematic and efficient adsorbent preparation strategies.
28962778	5	9	theme	indigo	904:909	arg1	carmine					911:917	indigo carmine	904:917	indigo carmine	904:917	Adsorption experiments reveal a synergistic effect between CS and HCP particles, and the samples are able to remove both anionic and cationic dyes (indigo carmine, rhodamine 6G and sunset yellow) from water.
28962778	5	9	theme	indigo	904:909	arg1	dyes					898:901	both anionic and cationic dyes	872:901	both anionic and cationic dyes (indigo carmine, rhodamine 6G and sunset yellow)	872:950	Adsorption experiments reveal a synergistic effect between CS and HCP particles, and the samples are able to remove both anionic and cationic dyes (indigo carmine, rhodamine 6G and sunset yellow) from water.
28962778	4	10	theme	HCP	608:610	arg1	particles					612:620	The HCP particles	604:620	The HCP particles	604:620	The HCP particles are well distributed and firmly embedded into the chitosan matrix and the composite hydrogels exhibit improved mechanical properties.
28962778	7	11	theme	adsorption	1190:1199	arg1	ability					1201:1207	their adsorption ability unaltered	1184:1217	their adsorption ability unaltered	1184:1217	Moreover, the mechanical properties of the composite hydrogels are enhanced respect to pure CS, and the samples can be regenerated and reused keeping their adsorption ability unaltered over successive cycles of adsorption, desorption, and washing.
28962778	2	12	link	hyper-crosslinked	373:389	arg1	particles					405:413	hyper-crosslinked polymer (HCP) particles	373:413	hyper-crosslinked polymer (HCP) particles	373:413	To address this challenge, we developed composite chitosan (CS)-based hydrogels containing hyper-crosslinked polymer (HCP) particles to be used as broad-spectrum adsorbents.
28962778	3	13	theme	porous	554:559	arg1	particles					561:569	the porous particles	550:569	the porous particles of trapping pollutant molecules	550:601	The goal is to efficiently combine the dye adsorption ability of chitosan and the capacity of the porous particles of trapping pollutant molecules.
28962778	3	14	theme	dye	495:497	arg1	ability					510:516	the dye adsorption ability	491:516	the dye adsorption ability of chitosan	491:528	The goal is to efficiently combine the dye adsorption ability of chitosan and the capacity of the porous particles of trapping pollutant molecules.
28962778	4	15	theme	mechanical	733:742	arg1	properties					744:753	improved mechanical properties	724:753	improved mechanical properties	724:753	The HCP particles are well distributed and firmly embedded into the chitosan matrix and the composite hydrogels exhibit improved mechanical properties.
28962778	4	16	theme	chitosan	672:679	arg1	matrix					681:686	the chitosan matrix	668:686	the chitosan matrix	668:686	The HCP particles are well distributed and firmly embedded into the chitosan matrix and the composite hydrogels exhibit improved mechanical properties.
28962778	6	17	theme	comparable	1010:1019	arg1	biosorbents					1021:1031	comparable biosorbents	1010:1031	comparable biosorbents	1010:1031	The maximum dye uptake is higher than that of comparable biosorbents.
28962778	7	18	theme	unaltered	1209:1217	arg1	ability					1201:1207	their adsorption ability unaltered	1184:1217	their adsorption ability unaltered	1184:1217	Moreover, the mechanical properties of the composite hydrogels are enhanced respect to pure CS, and the samples can be regenerated and reused keeping their adsorption ability unaltered over successive cycles of adsorption, desorption, and washing.
28962778	7	19	theme	composite	1077:1085	arg1	hydrogels					1087:1095	the composite hydrogels	1073:1095	the composite hydrogels	1073:1095	Moreover, the mechanical properties of the composite hydrogels are enhanced respect to pure CS, and the samples can be regenerated and reused keeping their adsorption ability unaltered over successive cycles of adsorption, desorption, and washing.
28962778	3	20	theme	trapping	574:581	arg1	molecules					593:601	trapping pollutant molecules	574:601	trapping pollutant molecules	574:601	The goal is to efficiently combine the dye adsorption ability of chitosan and the capacity of the porous particles of trapping pollutant molecules.
28962778	5	21	theme	cationic	889:896	arg1	carmine					911:917	indigo carmine	904:917	indigo carmine	904:917	Adsorption experiments reveal a synergistic effect between CS and HCP particles, and the samples are able to remove both anionic and cationic dyes (indigo carmine, rhodamine 6G and sunset yellow) from water.
28962778	5	21	theme	cationic	889:896	arg1	6G					930:931	rhodamine 6G	920:931	rhodamine 6G	920:931	Adsorption experiments reveal a synergistic effect between CS and HCP particles, and the samples are able to remove both anionic and cationic dyes (indigo carmine, rhodamine 6G and sunset yellow) from water.
28962778	5	21	theme	cationic	889:896	arg1	yellow					944:949	sunset yellow	937:949	sunset yellow	937:949	Adsorption experiments reveal a synergistic effect between CS and HCP particles, and the samples are able to remove both anionic and cationic dyes (indigo carmine, rhodamine 6G and sunset yellow) from water.
28962778	5	21	theme	cationic	889:896	arg1	dyes					898:901	both anionic and cationic dyes	872:901	both anionic and cationic dyes (indigo carmine, rhodamine 6G and sunset yellow)	872:950	Adsorption experiments reveal a synergistic effect between CS and HCP particles, and the samples are able to remove both anionic and cationic dyes (indigo carmine, rhodamine 6G and sunset yellow) from water.
28962778	6	22	theme	dye	976:978	arg1	higher					990:995	higher	990:995	higher	990:995	The maximum dye uptake is higher than that of comparable biosorbents.
28962778	6	22	theme	dye	976:978	arg1	uptake					980:985	The maximum dye uptake	964:985	The maximum dye uptake	964:985	The maximum dye uptake is higher than that of comparable biosorbents.
28962778	7	23	theme	hydrogels	1087:1095	arg1	properties					1059:1068	the mechanical properties	1044:1068	the mechanical properties of the composite hydrogels	1044:1095	Moreover, the mechanical properties of the composite hydrogels are enhanced respect to pure CS, and the samples can be regenerated and reused keeping their adsorption ability unaltered over successive cycles of adsorption, desorption, and washing.
28962778	5	24	theme	anionic	877:883	arg1	carmine					911:917	indigo carmine	904:917	indigo carmine	904:917	Adsorption experiments reveal a synergistic effect between CS and HCP particles, and the samples are able to remove both anionic and cationic dyes (indigo carmine, rhodamine 6G and sunset yellow) from water.
28962778	5	24	theme	anionic	877:883	arg1	6G					930:931	rhodamine 6G	920:931	rhodamine 6G	920:931	Adsorption experiments reveal a synergistic effect between CS and HCP particles, and the samples are able to remove both anionic and cationic dyes (indigo carmine, rhodamine 6G and sunset yellow) from water.
28962778	5	24	theme	anionic	877:883	arg1	yellow					944:949	sunset yellow	937:949	sunset yellow	937:949	Adsorption experiments reveal a synergistic effect between CS and HCP particles, and the samples are able to remove both anionic and cationic dyes (indigo carmine, rhodamine 6G and sunset yellow) from water.
28962778	5	24	theme	anionic	877:883	arg1	dyes					898:901	both anionic and cationic dyes	872:901	both anionic and cationic dyes (indigo carmine, rhodamine 6G and sunset yellow)	872:950	Adsorption experiments reveal a synergistic effect between CS and HCP particles, and the samples are able to remove both anionic and cationic dyes (indigo carmine, rhodamine 6G and sunset yellow) from water.
28962778	2	25	theme	chitosan	332:339	arg1	hydrogels					352:360	composite chitosan (CS)-based hydrogels	322:360	composite chitosan (CS)-based hydrogels containing hyper-crosslinked polymer (HCP) particles	322:413	To address this challenge, we developed composite chitosan (CS)-based hydrogels containing hyper-crosslinked polymer (HCP) particles to be used as broad-spectrum adsorbents.
28962778	2	26	theme	HCP	400:402	arg1	particles					405:413	hyper-crosslinked polymer (HCP) particles	373:413	hyper-crosslinked polymer (HCP) particles	373:413	To address this challenge, we developed composite chitosan (CS)-based hydrogels containing hyper-crosslinked polymer (HCP) particles to be used as broad-spectrum adsorbents.
28962778	0	27	theme	hyper-crosslinked	29:45	arg1	particles					55:63	embedding hyper-crosslinked polymer particles	19:63	embedding hyper-crosslinked polymer particles	19:63	Chitosan hydrogels embedding hyper-crosslinked polymer particles as reusable broad-spectrum adsorbents for dye removal.
28962778	0	27	theme	hyper-crosslinked	29:45	arg1	adsorbents					92:101	reusable broad-spectrum adsorbents	68:101	reusable broad-spectrum adsorbents for dye removal	68:117	Chitosan hydrogels embedding hyper-crosslinked polymer particles as reusable broad-spectrum adsorbents for dye removal.
28962778	6	28	theme	maximum	968:974	arg1	higher					990:995	higher	990:995	higher	990:995	The maximum dye uptake is higher than that of comparable biosorbents.
28962778	6	28	theme	maximum	968:974	arg1	uptake					980:985	The maximum dye uptake	964:985	The maximum dye uptake	964:985	The maximum dye uptake is higher than that of comparable biosorbents.
28962778	5	29	theme	sunset	937:942	arg1	yellow					944:949	sunset yellow	937:949	sunset yellow	937:949	Adsorption experiments reveal a synergistic effect between CS and HCP particles, and the samples are able to remove both anionic and cationic dyes (indigo carmine, rhodamine 6G and sunset yellow) from water.
28962778	5	29	theme	sunset	937:942	arg1	dyes					898:901	both anionic and cationic dyes	872:901	both anionic and cationic dyes (indigo carmine, rhodamine 6G and sunset yellow)	872:950	Adsorption experiments reveal a synergistic effect between CS and HCP particles, and the samples are able to remove both anionic and cationic dyes (indigo carmine, rhodamine 6G and sunset yellow) from water.
28962778	2	30	theme	composite	322:330	arg1	CS					342:343	CS	342:343	CS	342:343	To address this challenge, we developed composite chitosan (CS)-based hydrogels containing hyper-crosslinked polymer (HCP) particles to be used as broad-spectrum adsorbents.
28962778	2	30	theme	composite	322:330	arg1	chitosan					332:339	composite chitosan	322:339	composite chitosan (CS)-based hydrogels containing hyper-crosslinked polymer (HCP) particles	322:413	To address this challenge, we developed composite chitosan (CS)-based hydrogels containing hyper-crosslinked polymer (HCP) particles to be used as broad-spectrum adsorbents.
28962778	2	31	theme	broad-spectrum	429:442	arg1	adsorbents					444:453	broad-spectrum adsorbents	429:453	broad-spectrum adsorbents	429:453	To address this challenge, we developed composite chitosan (CS)-based hydrogels containing hyper-crosslinked polymer (HCP) particles to be used as broad-spectrum adsorbents.
28962778	0	32	theme	embedding	19:27	arg1	particles					55:63	embedding hyper-crosslinked polymer particles	19:63	embedding hyper-crosslinked polymer particles	19:63	Chitosan hydrogels embedding hyper-crosslinked polymer particles as reusable broad-spectrum adsorbents for dye removal.
28962778	0	32	theme	embedding	19:27	arg1	adsorbents					92:101	reusable broad-spectrum adsorbents	68:101	reusable broad-spectrum adsorbents for dye removal	68:117	Chitosan hydrogels embedding hyper-crosslinked polymer particles as reusable broad-spectrum adsorbents for dye removal.
28962778	4	33	theme	composite	696:704	arg1	hydrogels					706:714	the composite hydrogels	692:714	the composite hydrogels	692:714	The HCP particles are well distributed and firmly embedded into the chitosan matrix and the composite hydrogels exhibit improved mechanical properties.
28962778	2	34	theme	-based	345:350	arg1	hydrogels					352:360	composite chitosan (CS)-based hydrogels	322:360	composite chitosan (CS)-based hydrogels containing hyper-crosslinked polymer (HCP) particles	322:413	To address this challenge, we developed composite chitosan (CS)-based hydrogels containing hyper-crosslinked polymer (HCP) particles to be used as broad-spectrum adsorbents.
28962778	7	35	theme	mechanical	1048:1057	arg1	properties					1059:1068	the mechanical properties	1044:1068	the mechanical properties of the composite hydrogels	1044:1095	Moreover, the mechanical properties of the composite hydrogels are enhanced respect to pure CS, and the samples can be regenerated and reused keeping their adsorption ability unaltered over successive cycles of adsorption, desorption, and washing.
28962778	7	36	theme	adsorption	1245:1254	arg1	cycles					1235:1240	successive cycles	1224:1240	successive cycles of adsorption, desorption, and washing	1224:1279	Moreover, the mechanical properties of the composite hydrogels are enhanced respect to pure CS, and the samples can be regenerated and reused keeping their adsorption ability unaltered over successive cycles of adsorption, desorption, and washing.
28962778	1	37	theme	important	193:201	arg1	issue					203:207	an extremely important issue	180:207	an extremely important issue that requires systematic and efficient adsorbent preparation strategies	180:279	The removal of dye and toxic ionic pollutants from water is an extremely important issue that requires systematic and efficient adsorbent preparation strategies.
28962778	1	37	theme	important	193:201	arg1	removal					124:130	The removal	120:130	The removal of dye and toxic ionic pollutants from water	120:175	The removal of dye and toxic ionic pollutants from water is an extremely important issue that requires systematic and efficient adsorbent preparation strategies.
28962778	3	38	theme	pollutant	583:591	arg1	molecules					593:601	trapping pollutant molecules	574:601	trapping pollutant molecules	574:601	The goal is to efficiently combine the dye adsorption ability of chitosan and the capacity of the porous particles of trapping pollutant molecules.
28962778	0	39	theme	polymer	47:53	arg1	particles					55:63	embedding hyper-crosslinked polymer particles	19:63	embedding hyper-crosslinked polymer particles	19:63	Chitosan hydrogels embedding hyper-crosslinked polymer particles as reusable broad-spectrum adsorbents for dye removal.
28962778	0	39	theme	polymer	47:53	arg1	adsorbents					92:101	reusable broad-spectrum adsorbents	68:101	reusable broad-spectrum adsorbents for dye removal	68:117	Chitosan hydrogels embedding hyper-crosslinked polymer particles as reusable broad-spectrum adsorbents for dye removal.
28962778	5	40	theme	HCP	822:824	arg1	particles					826:834	CS and HCP particles	815:834	CS and HCP particles	815:834	Adsorption experiments reveal a synergistic effect between CS and HCP particles, and the samples are able to remove both anionic and cationic dyes (indigo carmine, rhodamine 6G and sunset yellow) from water.
28962778	4	41	theme	improved	724:731	arg1	properties					744:753	improved mechanical properties	724:753	improved mechanical properties	724:753	The HCP particles are well distributed and firmly embedded into the chitosan matrix and the composite hydrogels exhibit improved mechanical properties.
28962778	5	42	dep	dyes	898:901	arg1	carmine					911:917	indigo carmine	904:917	indigo carmine	904:917	Adsorption experiments reveal a synergistic effect between CS and HCP particles, and the samples are able to remove both anionic and cationic dyes (indigo carmine, rhodamine 6G and sunset yellow) from water.
28962778	5	42	dep	dyes	898:901	arg1	6G					930:931	rhodamine 6G	920:931	rhodamine 6G	920:931	Adsorption experiments reveal a synergistic effect between CS and HCP particles, and the samples are able to remove both anionic and cationic dyes (indigo carmine, rhodamine 6G and sunset yellow) from water.
28962778	5	42	dep	dyes	898:901	arg1	yellow					944:949	sunset yellow	937:949	sunset yellow	937:949	Adsorption experiments reveal a synergistic effect between CS and HCP particles, and the samples are able to remove both anionic and cationic dyes (indigo carmine, rhodamine 6G and sunset yellow) from water.
28962778	5	42	dep	dyes	898:901	arg1	dyes					898:901	both anionic and cationic dyes	872:901	both anionic and cationic dyes (indigo carmine, rhodamine 6G and sunset yellow)	872:950	Adsorption experiments reveal a synergistic effect between CS and HCP particles, and the samples are able to remove both anionic and cationic dyes (indigo carmine, rhodamine 6G and sunset yellow) from water.
28962778	1	43	theme	systematic	223:232	arg1	strategies					270:279	systematic and efficient adsorbent preparation strategies	223:279	systematic and efficient adsorbent preparation strategies	223:279	The removal of dye and toxic ionic pollutants from water is an extremely important issue that requires systematic and efficient adsorbent preparation strategies.
28962778	0	44	theme	reusable	68:75	arg1	particles					55:63	embedding hyper-crosslinked polymer particles	19:63	embedding hyper-crosslinked polymer particles	19:63	Chitosan hydrogels embedding hyper-crosslinked polymer particles as reusable broad-spectrum adsorbents for dye removal.
28962778	0	44	theme	reusable	68:75	arg1	adsorbents					92:101	reusable broad-spectrum adsorbents	68:101	reusable broad-spectrum adsorbents for dye removal	68:117	Chitosan hydrogels embedding hyper-crosslinked polymer particles as reusable broad-spectrum adsorbents for dye removal.
28962778	7	45	theme	successive	1224:1233	arg1	cycles					1235:1240	successive cycles	1224:1240	successive cycles of adsorption, desorption, and washing	1224:1279	Moreover, the mechanical properties of the composite hydrogels are enhanced respect to pure CS, and the samples can be regenerated and reused keeping their adsorption ability unaltered over successive cycles of adsorption, desorption, and washing.
28962778	3	46	theme	molecules	593:601	arg1	particles					561:569	the porous particles	550:569	the porous particles of trapping pollutant molecules	550:601	The goal is to efficiently combine the dye adsorption ability of chitosan and the capacity of the porous particles of trapping pollutant molecules.
28962778	3	47	theme	adsorption	499:508	arg1	ability					510:516	the dye adsorption ability	491:516	the dye adsorption ability of chitosan	491:528	The goal is to efficiently combine the dye adsorption ability of chitosan and the capacity of the porous particles of trapping pollutant molecules.
28962778	1	48	theme	pollutants	155:164	arg1	issue					203:207	an extremely important issue	180:207	an extremely important issue that requires systematic and efficient adsorbent preparation strategies	180:279	The removal of dye and toxic ionic pollutants from water is an extremely important issue that requires systematic and efficient adsorbent preparation strategies.
28962778	1	48	theme	pollutants	155:164	arg1	removal					124:130	The removal	120:130	The removal of dye and toxic ionic pollutants from water	120:175	The removal of dye and toxic ionic pollutants from water is an extremely important issue that requires systematic and efficient adsorbent preparation strategies.
28962778	1	49	theme	dye	135:137	arg1	issue					203:207	an extremely important issue	180:207	an extremely important issue that requires systematic and efficient adsorbent preparation strategies	180:279	The removal of dye and toxic ionic pollutants from water is an extremely important issue that requires systematic and efficient adsorbent preparation strategies.
28962778	1	49	theme	dye	135:137	arg1	removal					124:130	The removal	120:130	The removal of dye and toxic ionic pollutants from water	120:175	The removal of dye and toxic ionic pollutants from water is an extremely important issue that requires systematic and efficient adsorbent preparation strategies.
28962778	7	50	theme	desorption	1257:1266	arg1	cycles					1235:1240	successive cycles	1224:1240	successive cycles of adsorption, desorption, and washing	1224:1279	Moreover, the mechanical properties of the composite hydrogels are enhanced respect to pure CS, and the samples can be regenerated and reused keeping their adsorption ability unaltered over successive cycles of adsorption, desorption, and washing.
28962778	3	51	theme	particles	561:569	arg1	capacity					538:545	the capacity	534:545	the capacity of the porous particles of trapping pollutant molecules	534:601	The goal is to efficiently combine the dye adsorption ability of chitosan and the capacity of the porous particles of trapping pollutant molecules.
28962778	3	51	theme	particles	561:569	arg1	ability					510:516	the dye adsorption ability	491:516	the dye adsorption ability of chitosan	491:528	The goal is to efficiently combine the dye adsorption ability of chitosan and the capacity of the porous particles of trapping pollutant molecules.
28962778	5	52	theme	rhodamine	920:928	arg1	6G					930:931	rhodamine 6G	920:931	rhodamine 6G	920:931	Adsorption experiments reveal a synergistic effect between CS and HCP particles, and the samples are able to remove both anionic and cationic dyes (indigo carmine, rhodamine 6G and sunset yellow) from water.
28962778	5	52	theme	rhodamine	920:928	arg1	dyes					898:901	both anionic and cationic dyes	872:901	both anionic and cationic dyes (indigo carmine, rhodamine 6G and sunset yellow)	872:950	Adsorption experiments reveal a synergistic effect between CS and HCP particles, and the samples are able to remove both anionic and cationic dyes (indigo carmine, rhodamine 6G and sunset yellow) from water.
28962778	1	53	theme	toxic	143:147	arg1	pollutants					155:164	toxic ionic pollutants	143:164	toxic ionic pollutants	143:164	The removal of dye and toxic ionic pollutants from water is an extremely important issue that requires systematic and efficient adsorbent preparation strategies.
28962778	1	54	theme	efficient	238:246	arg1	strategies					270:279	systematic and efficient adsorbent preparation strategies	223:279	systematic and efficient adsorbent preparation strategies	223:279	The removal of dye and toxic ionic pollutants from water is an extremely important issue that requires systematic and efficient adsorbent preparation strategies.
28962778	5	55	theme	synergistic	788:798	arg1	effect					800:805	a synergistic effect	786:805	a synergistic effect between CS and HCP particles	786:834	Adsorption experiments reveal a synergistic effect between CS and HCP particles, and the samples are able to remove both anionic and cationic dyes (indigo carmine, rhodamine 6G and sunset yellow) from water.
28962778	1	56	theme	ionic	149:153	arg1	pollutants					155:164	toxic ionic pollutants	143:164	toxic ionic pollutants	143:164	The removal of dye and toxic ionic pollutants from water is an extremely important issue that requires systematic and efficient adsorbent preparation strategies.
28962778	1	57	theme	adsorbent	248:256	arg1	strategies					270:279	systematic and efficient adsorbent preparation strategies	223:279	systematic and efficient adsorbent preparation strategies	223:279	The removal of dye and toxic ionic pollutants from water is an extremely important issue that requires systematic and efficient adsorbent preparation strategies.
28962778	0	58	theme	broad-spectrum	77:90	arg1	particles					55:63	embedding hyper-crosslinked polymer particles	19:63	embedding hyper-crosslinked polymer particles	19:63	Chitosan hydrogels embedding hyper-crosslinked polymer particles as reusable broad-spectrum adsorbents for dye removal.
28962778	0	58	theme	broad-spectrum	77:90	arg1	adsorbents					92:101	reusable broad-spectrum adsorbents	68:101	reusable broad-spectrum adsorbents for dye removal	68:117	Chitosan hydrogels embedding hyper-crosslinked polymer particles as reusable broad-spectrum adsorbents for dye removal.
28962778	7	59	theme	washing	1273:1279	arg1	cycles					1235:1240	successive cycles	1224:1240	successive cycles of adsorption, desorption, and washing	1224:1279	Moreover, the mechanical properties of the composite hydrogels are enhanced respect to pure CS, and the samples can be regenerated and reused keeping their adsorption ability unaltered over successive cycles of adsorption, desorption, and washing.
28962778	2	60	theme	polymer	391:397	arg1	particles					405:413	hyper-crosslinked polymer (HCP) particles	373:413	hyper-crosslinked polymer (HCP) particles	373:413	To address this challenge, we developed composite chitosan (CS)-based hydrogels containing hyper-crosslinked polymer (HCP) particles to be used as broad-spectrum adsorbents.
28962778	1	61	theme	preparation	258:268	arg1	strategies					270:279	systematic and efficient adsorbent preparation strategies	223:279	systematic and efficient adsorbent preparation strategies	223:279	The removal of dye and toxic ionic pollutants from water is an extremely important issue that requires systematic and efficient adsorbent preparation strategies.
25178064	4	0	theme	hot	462:464	arg1	extraction					472:481	hot water extraction	462:481	hot water extraction	462:481	In this work, a crude polysaccharide of M. charantia (MCP) fruit was isolated by hot water extraction and then purified using DEAE-52 cellulose anion-exchange chromatography to produce two main fractions MCP1 and MCP2.
25178064	5	1	theme	physicochemical	633:647	arg1	characteristics					649:663	physicochemical characteristics	633:663	physicochemical characteristics	633:663	The immunomodulatory effects and physicochemical characteristics of these fractions were investigated in vitro and in vivo.
25178064	6	2	theme	cyclophosphamide	980:995	arg1	mice					1027:1030	cyclophosphamide (Cy)-induced immunosuppressed mice	980:1030	cyclophosphamide (Cy)-induced immunosuppressed mice	980:1030	The results showed that intragastric administration of 150 or 300 mg·kg-·d⁻¹ of MCP significantly increased the carbolic particle clearance index, serum haemolysin production, spleen index, thymus index and NK cell cytotoxicity to normal control levels in cyclophosphamide (Cy)-induced immunosuppressed mice.
25178064	0	3	from	activity	17:24	arg1	charantia					89:97	Momordica charantia	79:97	Momordica charantia	79:97	Immunomodulatory activity and partial characterisation of polysaccharides from Momordica charantia.
25178064	3	4	theme	related	236:242	arg1	activities					244:253	related activities	236:253	related activities	236:253	Until now, studies on its composition and related activities have been confined to compounds of low molecular mass, and no data have been reported concerning the plant's polysaccharides.
25178064	6	5	theme	haemolysin	877:886	arg1	production					888:897	serum haemolysin production	871:897	serum haemolysin production	871:897	The results showed that intragastric administration of 150 or 300 mg·kg-·d⁻¹ of MCP significantly increased the carbolic particle clearance index, serum haemolysin production, spleen index, thymus index and NK cell cytotoxicity to normal control levels in cyclophosphamide (Cy)-induced immunosuppressed mice.
25178064	8	6	theme	molecular	1181:1189	arg1	weights					1191:1197	The average molecular weights	1169:1197	The average molecular weights of MCP1 and MCP2, which were measured using high-performance gel permeation chromatography,	1169:1289	The average molecular weights of MCP1 and MCP2, which were measured using high-performance gel permeation chromatography, were 8.55×10⁴ Da and 4.41×10⁵ Da, respectively.
25178064	8	6	theme	molecular	1181:1189	arg1	Da					1305:1306	8.55×10⁴ Da	1296:1306	8.55×10⁴ Da	1296:1306	The average molecular weights of MCP1 and MCP2, which were measured using high-performance gel permeation chromatography, were 8.55×10⁴ Da and 4.41×10⁵ Da, respectively.
25178064	8	7	theme	average	1173:1179	arg1	weights					1191:1197	The average molecular weights	1169:1197	The average molecular weights of MCP1 and MCP2, which were measured using high-performance gel permeation chromatography,	1169:1289	The average molecular weights of MCP1 and MCP2, which were measured using high-performance gel permeation chromatography, were 8.55×10⁴ Da and 4.41×10⁵ Da, respectively.
25178064	8	7	theme	average	1173:1179	arg1	Da					1305:1306	8.55×10⁴ Da	1296:1306	8.55×10⁴ Da	1296:1306	The average molecular weights of MCP1 and MCP2, which were measured using high-performance gel permeation chromatography, were 8.55×10⁴ Da and 4.41×10⁵ Da, respectively.
25178064	9	8	dep	Fourier	1409:1415	arg1	transform					1417:1425	transform	1417:1425	transform infrared spectrum	1417:1443	Both fractions exhibited characteristic polysaccharide bands in their Fourier transform infrared spectrum.
25178064	6	9	theme	MCP	804:806	arg1	mg·kg-·d⁻¹					790:799	150 or 300 mg·kg-·d⁻¹	779:799	150 or 300 mg·kg-·d⁻¹ of MCP	779:806	The results showed that intragastric administration of 150 or 300 mg·kg-·d⁻¹ of MCP significantly increased the carbolic particle clearance index, serum haemolysin production, spleen index, thymus index and NK cell cytotoxicity to normal control levels in cyclophosphamide (Cy)-induced immunosuppressed mice.
25178064	4	10	theme	main	570:573	arg1	fractions					575:583	two main fractions	566:583	two main fractions MCP1 and MCP2	566:597	In this work, a crude polysaccharide of M. charantia (MCP) fruit was isolated by hot water extraction and then purified using DEAE-52 cellulose anion-exchange chromatography to produce two main fractions MCP1 and MCP2.
25178064	4	10	theme	main	570:573	arg1	MCP2					594:597	MCP2	594:597	MCP2	594:597	In this work, a crude polysaccharide of M. charantia (MCP) fruit was isolated by hot water extraction and then purified using DEAE-52 cellulose anion-exchange chromatography to produce two main fractions MCP1 and MCP2.
25178064	4	10	theme	main	570:573	arg1	MCP1					585:588	MCP1	585:588	MCP1	585:588	In this work, a crude polysaccharide of M. charantia (MCP) fruit was isolated by hot water extraction and then purified using DEAE-52 cellulose anion-exchange chromatography to produce two main fractions MCP1 and MCP2.
25178064	4	11	theme	fruit	440:444	arg1	polysaccharide					403:416	a crude polysaccharide	395:416	a crude polysaccharide of M. charantia (MCP) fruit	395:444	In this work, a crude polysaccharide of M. charantia (MCP) fruit was isolated by hot water extraction and then purified using DEAE-52 cellulose anion-exchange chromatography to produce two main fractions MCP1 and MCP2.
25178064	7	12	theme	splenic	1109:1115	arg1	proliferation					1128:1140	concanavalin A-induced splenic lymphocyte proliferation	1086:1140	concanavalin A-induced splenic lymphocyte proliferation	1086:1140	Both MCP1 and MCP2 effectively stimulated normal and concanavalin A-induced splenic lymphocyte proliferation in vitro at various doses.
25178064	8	13	theme	gel	1260:1262	arg1	chromatography					1275:1288	high-performance gel permeation chromatography	1243:1288	high-performance gel permeation chromatography	1243:1288	The average molecular weights of MCP1 and MCP2, which were measured using high-performance gel permeation chromatography, were 8.55×10⁴ Da and 4.41×10⁵ Da, respectively.
25178064	0	14	theme	polysaccharides	58:72	arg1	activity					17:24	Immunomodulatory activity	0:24	Immunomodulatory activity	0:24	Immunomodulatory activity and partial characterisation of polysaccharides from Momordica charantia.
25178064	0	14	theme	polysaccharides	58:72	arg1	characterisation					38:53	partial characterisation	30:53	partial characterisation	30:53	Immunomodulatory activity and partial characterisation of polysaccharides from Momordica charantia.
25178064	2	15	theme	medicinal	151:159	arg1	vegetable					161:169	an edible and medicinal vegetable	137:169	an edible and medicinal vegetable in sub-tropical areas	137:191	is used as an edible and medicinal vegetable in sub-tropical areas.
25178064	5	16	theme	immunomodulatory	604:619	arg1	effects					621:627	immunomodulatory effects	604:627	immunomodulatory effects	604:627	The immunomodulatory effects and physicochemical characteristics of these fractions were investigated in vitro and in vivo.
25178064	6	17	theme	mg·kg-·d⁻¹	790:799	arg1	administration					761:774	intragastric administration	748:774	intragastric administration of 150 or 300 mg·kg-·d⁻¹ of MCP	748:806	The results showed that intragastric administration of 150 or 300 mg·kg-·d⁻¹ of MCP significantly increased the carbolic particle clearance index, serum haemolysin production, spleen index, thymus index and NK cell cytotoxicity to normal control levels in cyclophosphamide (Cy)-induced immunosuppressed mice.
25178064	8	18	theme	MCP1	1202:1205	arg1	weights					1191:1197	The average molecular weights	1169:1197	The average molecular weights of MCP1 and MCP2, which were measured using high-performance gel permeation chromatography,	1169:1289	The average molecular weights of MCP1 and MCP2, which were measured using high-performance gel permeation chromatography, were 8.55×10⁴ Da and 4.41×10⁵ Da, respectively.
25178064	8	18	theme	MCP1	1202:1205	arg1	Da					1305:1306	8.55×10⁴ Da	1296:1306	8.55×10⁴ Da	1296:1306	The average molecular weights of MCP1 and MCP2, which were measured using high-performance gel permeation chromatography, were 8.55×10⁴ Da and 4.41×10⁵ Da, respectively.
25178064	8	19	theme	high-performance	1243:1258	arg1	chromatography					1275:1288	high-performance gel permeation chromatography	1243:1288	high-performance gel permeation chromatography	1243:1288	The average molecular weights of MCP1 and MCP2, which were measured using high-performance gel permeation chromatography, were 8.55×10⁴ Da and 4.41×10⁵ Da, respectively.
25178064	0	20	from	characterisation	38:53	arg1	charantia					89:97	Momordica charantia	79:97	Momordica charantia	79:97	Immunomodulatory activity and partial characterisation of polysaccharides from Momordica charantia.
25178064	7	21	theme	concanavalin	1086:1097	arg1	proliferation					1128:1140	concanavalin A-induced splenic lymphocyte proliferation	1086:1140	concanavalin A-induced splenic lymphocyte proliferation	1086:1140	Both MCP1 and MCP2 effectively stimulated normal and concanavalin A-induced splenic lymphocyte proliferation in vitro at various doses.
25178064	6	22	theme	thymus	914:919	arg1	index					921:925	thymus index	914:925	thymus index	914:925	The results showed that intragastric administration of 150 or 300 mg·kg-·d⁻¹ of MCP significantly increased the carbolic particle clearance index, serum haemolysin production, spleen index, thymus index and NK cell cytotoxicity to normal control levels in cyclophosphamide (Cy)-induced immunosuppressed mice.
25178064	6	23	theme	spleen	900:905	arg1	index					907:911	spleen index	900:911	spleen index	900:911	The results showed that intragastric administration of 150 or 300 mg·kg-·d⁻¹ of MCP significantly increased the carbolic particle clearance index, serum haemolysin production, spleen index, thymus index and NK cell cytotoxicity to normal control levels in cyclophosphamide (Cy)-induced immunosuppressed mice.
25178064	9	24	dep	transform	1417:1425	arg1	infrared					1427:1434	infrared	1427:1434	transform infrared spectrum	1417:1443	Both fractions exhibited characteristic polysaccharide bands in their Fourier transform infrared spectrum.
25178064	0	25	theme	Immunomodulatory	0:15	arg1	activity					17:24	Immunomodulatory activity	0:24	Immunomodulatory activity	0:24	Immunomodulatory activity and partial characterisation of polysaccharides from Momordica charantia.
25178064	4	26	theme	anion-exchange	525:538	arg1	chromatography					540:553	DEAE-52 cellulose anion-exchange chromatography	507:553	DEAE-52 cellulose anion-exchange chromatography	507:553	In this work, a crude polysaccharide of M. charantia (MCP) fruit was isolated by hot water extraction and then purified using DEAE-52 cellulose anion-exchange chromatography to produce two main fractions MCP1 and MCP2.
25178064	7	27	theme	A-induced	1099:1107	arg1	proliferation					1128:1140	concanavalin A-induced splenic lymphocyte proliferation	1086:1140	concanavalin A-induced splenic lymphocyte proliferation	1086:1140	Both MCP1 and MCP2 effectively stimulated normal and concanavalin A-induced splenic lymphocyte proliferation in vitro at various doses.
25178064	6	28	theme	serum	871:875	arg1	production					888:897	serum haemolysin production	871:897	serum haemolysin production	871:897	The results showed that intragastric administration of 150 or 300 mg·kg-·d⁻¹ of MCP significantly increased the carbolic particle clearance index, serum haemolysin production, spleen index, thymus index and NK cell cytotoxicity to normal control levels in cyclophosphamide (Cy)-induced immunosuppressed mice.
25178064	0	29	theme	partial	30:36	arg1	characterisation					38:53	partial characterisation	30:53	partial characterisation	30:53	Immunomodulatory activity and partial characterisation of polysaccharides from Momordica charantia.
25178064	4	30	theme	cellulose	515:523	arg1	chromatography					540:553	DEAE-52 cellulose anion-exchange chromatography	507:553	DEAE-52 cellulose anion-exchange chromatography	507:553	In this work, a crude polysaccharide of M. charantia (MCP) fruit was isolated by hot water extraction and then purified using DEAE-52 cellulose anion-exchange chromatography to produce two main fractions MCP1 and MCP2.
25178064	7	31	theme	lymphocyte	1117:1126	arg1	proliferation					1128:1140	concanavalin A-induced splenic lymphocyte proliferation	1086:1140	concanavalin A-induced splenic lymphocyte proliferation	1086:1140	Both MCP1 and MCP2 effectively stimulated normal and concanavalin A-induced splenic lymphocyte proliferation in vitro at various doses.
25178064	8	32	theme	MCP2	1211:1214	arg1	weights					1191:1197	The average molecular weights	1169:1197	The average molecular weights of MCP1 and MCP2, which were measured using high-performance gel permeation chromatography,	1169:1289	The average molecular weights of MCP1 and MCP2, which were measured using high-performance gel permeation chromatography, were 8.55×10⁴ Da and 4.41×10⁵ Da, respectively.
25178064	8	32	theme	MCP2	1211:1214	arg1	Da					1305:1306	8.55×10⁴ Da	1296:1306	8.55×10⁴ Da	1296:1306	The average molecular weights of MCP1 and MCP2, which were measured using high-performance gel permeation chromatography, were 8.55×10⁴ Da and 4.41×10⁵ Da, respectively.
25178064	6	33	theme	control	962:968	arg1	levels					970:975	normal control levels	955:975	normal control levels	955:975	The results showed that intragastric administration of 150 or 300 mg·kg-·d⁻¹ of MCP significantly increased the carbolic particle clearance index, serum haemolysin production, spleen index, thymus index and NK cell cytotoxicity to normal control levels in cyclophosphamide (Cy)-induced immunosuppressed mice.
25178064	3	34	theme	low	290:292	arg1	mass					304:307	low molecular mass	290:307	low molecular mass	290:307	Until now, studies on its composition and related activities have been confined to compounds of low molecular mass, and no data have been reported concerning the plant's polysaccharides.
25178064	4	35	dep	fractions	575:583	arg1	fractions					575:583	two main fractions	566:583	two main fractions MCP1 and MCP2	566:597	In this work, a crude polysaccharide of M. charantia (MCP) fruit was isolated by hot water extraction and then purified using DEAE-52 cellulose anion-exchange chromatography to produce two main fractions MCP1 and MCP2.
25178064	4	35	dep	fractions	575:583	arg1	MCP2					594:597	MCP2	594:597	MCP2	594:597	In this work, a crude polysaccharide of M. charantia (MCP) fruit was isolated by hot water extraction and then purified using DEAE-52 cellulose anion-exchange chromatography to produce two main fractions MCP1 and MCP2.
25178064	4	35	dep	fractions	575:583	arg1	MCP1					585:588	MCP1	585:588	MCP1	585:588	In this work, a crude polysaccharide of M. charantia (MCP) fruit was isolated by hot water extraction and then purified using DEAE-52 cellulose anion-exchange chromatography to produce two main fractions MCP1 and MCP2.
25178064	5	36	dep	effects	621:627	arg1	The					600:602	The	600:602	The	600:602	The immunomodulatory effects and physicochemical characteristics of these fractions were investigated in vitro and in vivo.
25178064	6	37	theme	normal	955:960	arg1	levels					970:975	normal control levels	955:975	normal control levels	955:975	The results showed that intragastric administration of 150 or 300 mg·kg-·d⁻¹ of MCP significantly increased the carbolic particle clearance index, serum haemolysin production, spleen index, thymus index and NK cell cytotoxicity to normal control levels in cyclophosphamide (Cy)-induced immunosuppressed mice.
25178064	4	38	theme	charantia	424:432	arg1	fruit					440:444	M. charantia (MCP) fruit	421:444	M. charantia (MCP) fruit	421:444	In this work, a crude polysaccharide of M. charantia (MCP) fruit was isolated by hot water extraction and then purified using DEAE-52 cellulose anion-exchange chromatography to produce two main fractions MCP1 and MCP2.
25178064	8	39	theme	permeation	1264:1273	arg1	chromatography					1275:1288	high-performance gel permeation chromatography	1243:1288	high-performance gel permeation chromatography	1243:1288	The average molecular weights of MCP1 and MCP2, which were measured using high-performance gel permeation chromatography, were 8.55×10⁴ Da and 4.41×10⁵ Da, respectively.
25178064	9	40	theme	polysaccharide	1379:1392	arg1	bands					1394:1398	characteristic polysaccharide bands	1364:1398	characteristic polysaccharide bands	1364:1398	Both fractions exhibited characteristic polysaccharide bands in their Fourier transform infrared spectrum.
25178064	0	41	from	charantia	89:97	arg1	polysaccharides					58:72	polysaccharides	58:72	polysaccharides from Momordica charantia	58:97	Immunomodulatory activity and partial characterisation of polysaccharides from Momordica charantia.
25178064	0	41	from	charantia	89:97	arg1	activity					17:24	Immunomodulatory activity	0:24	Immunomodulatory activity	0:24	Immunomodulatory activity and partial characterisation of polysaccharides from Momordica charantia.
25178064	0	41	from	charantia	89:97	arg1	characterisation					38:53	partial characterisation	30:53	partial characterisation	30:53	Immunomodulatory activity and partial characterisation of polysaccharides from Momordica charantia.
25178064	9	42	theme	characteristic	1364:1377	arg1	bands					1394:1398	characteristic polysaccharide bands	1364:1398	characteristic polysaccharide bands	1364:1398	Both fractions exhibited characteristic polysaccharide bands in their Fourier transform infrared spectrum.
25178064	8	43	theme	8.55×10⁴	1296:1303	arg1	weights					1191:1197	The average molecular weights	1169:1197	The average molecular weights of MCP1 and MCP2, which were measured using high-performance gel permeation chromatography,	1169:1289	The average molecular weights of MCP1 and MCP2, which were measured using high-performance gel permeation chromatography, were 8.55×10⁴ Da and 4.41×10⁵ Da, respectively.
25178064	8	43	theme	8.55×10⁴	1296:1303	arg1	Da					1305:1306	8.55×10⁴ Da	1296:1306	8.55×10⁴ Da	1296:1306	The average molecular weights of MCP1 and MCP2, which were measured using high-performance gel permeation chromatography, were 8.55×10⁴ Da and 4.41×10⁵ Da, respectively.
25178064	7	44	theme	various	1154:1160	arg1	doses					1162:1166	various doses	1154:1166	various doses	1154:1166	Both MCP1 and MCP2 effectively stimulated normal and concanavalin A-induced splenic lymphocyte proliferation in vitro at various doses.
25178064	11	45	contain	have	1607:1610	arg2	adjuvants					1648:1656	immunotherapeutic adjuvants	1630:1656	immunotherapeutic adjuvants	1630:1656	The results indicate that MCP and its fractions have good potential as immunotherapeutic adjuvants.
25178064	11	45	contain	have	1607:1610	arg1	fractions					1597:1605	its fractions	1593:1605	its fractions	1593:1605	The results indicate that MCP and its fractions have good potential as immunotherapeutic adjuvants.
25178064	11	45	contain	have	1607:1610	arg1	MCP					1585:1587	MCP	1585:1587	MCP	1585:1587	The results indicate that MCP and its fractions have good potential as immunotherapeutic adjuvants.
25178064	11	45	contain	have	1607:1610	arg2	potential					1617:1625	good potential	1612:1625	good potential	1612:1625	The results indicate that MCP and its fractions have good potential as immunotherapeutic adjuvants.
25178064	5	46	theme	fractions	674:682	arg1	effects					621:627	immunomodulatory effects	604:627	immunomodulatory effects	604:627	The immunomodulatory effects and physicochemical characteristics of these fractions were investigated in vitro and in vivo.
25178064	5	46	theme	fractions	674:682	arg1	characteristics					649:663	physicochemical characteristics	633:663	physicochemical characteristics	633:663	The immunomodulatory effects and physicochemical characteristics of these fractions were investigated in vitro and in vivo.
25178064	2	47	theme	sub-tropical	174:185	arg1	areas					187:191	sub-tropical areas	174:191	sub-tropical areas	174:191	is used as an edible and medicinal vegetable in sub-tropical areas.
25178064	8	48	theme	4.41×10⁵	1312:1319	arg1	Da					1321:1322	4.41×10⁵ Da	1312:1322	4.41×10⁵ Da	1312:1322	The average molecular weights of MCP1 and MCP2, which were measured using high-performance gel permeation chromatography, were 8.55×10⁴ Da and 4.41×10⁵ Da, respectively.
25178064	2	49	from	vegetable	161:169	arg1	areas					187:191	sub-tropical areas	174:191	sub-tropical areas	174:191	is used as an edible and medicinal vegetable in sub-tropical areas.
25178064	6	50	theme	cell	934:937	arg1	cytotoxicity					939:950	NK cell cytotoxicity	931:950	NK cell cytotoxicity	931:950	The results showed that intragastric administration of 150 or 300 mg·kg-·d⁻¹ of MCP significantly increased the carbolic particle clearance index, serum haemolysin production, spleen index, thymus index and NK cell cytotoxicity to normal control levels in cyclophosphamide (Cy)-induced immunosuppressed mice.
25178064	4	51	theme	water	466:470	arg1	extraction					472:481	hot water extraction	462:481	hot water extraction	462:481	In this work, a crude polysaccharide of M. charantia (MCP) fruit was isolated by hot water extraction and then purified using DEAE-52 cellulose anion-exchange chromatography to produce two main fractions MCP1 and MCP2.
25178064	6	52	theme	clearance	854:862	arg1	index					864:868	the carbolic particle clearance index	832:868	the carbolic particle clearance index	832:868	The results showed that intragastric administration of 150 or 300 mg·kg-·d⁻¹ of MCP significantly increased the carbolic particle clearance index, serum haemolysin production, spleen index, thymus index and NK cell cytotoxicity to normal control levels in cyclophosphamide (Cy)-induced immunosuppressed mice.
25178064	6	53	theme	particle	845:852	arg1	index					864:868	the carbolic particle clearance index	832:868	the carbolic particle clearance index	832:868	The results showed that intragastric administration of 150 or 300 mg·kg-·d⁻¹ of MCP significantly increased the carbolic particle clearance index, serum haemolysin production, spleen index, thymus index and NK cell cytotoxicity to normal control levels in cyclophosphamide (Cy)-induced immunosuppressed mice.
25178064	6	54	theme	intragastric	748:759	arg1	administration					761:774	intragastric administration	748:774	intragastric administration of 150 or 300 mg·kg-·d⁻¹ of MCP	748:806	The results showed that intragastric administration of 150 or 300 mg·kg-·d⁻¹ of MCP significantly increased the carbolic particle clearance index, serum haemolysin production, spleen index, thymus index and NK cell cytotoxicity to normal control levels in cyclophosphamide (Cy)-induced immunosuppressed mice.
25178064	6	55	theme	NK	931:932	arg1	cytotoxicity					939:950	NK cell cytotoxicity	931:950	NK cell cytotoxicity	931:950	The results showed that intragastric administration of 150 or 300 mg·kg-·d⁻¹ of MCP significantly increased the carbolic particle clearance index, serum haemolysin production, spleen index, thymus index and NK cell cytotoxicity to normal control levels in cyclophosphamide (Cy)-induced immunosuppressed mice.
25178064	3	56	theme	molecular	294:302	arg1	mass					304:307	low molecular mass	290:307	low molecular mass	290:307	Until now, studies on its composition and related activities have been confined to compounds of low molecular mass, and no data have been reported concerning the plant's polysaccharides.
25178064	4	57	theme	crude	397:401	arg1	polysaccharide					403:416	a crude polysaccharide	395:416	a crude polysaccharide of M. charantia (MCP) fruit	395:444	In this work, a crude polysaccharide of M. charantia (MCP) fruit was isolated by hot water extraction and then purified using DEAE-52 cellulose anion-exchange chromatography to produce two main fractions MCP1 and MCP2.
25178064	11	58	theme	good	1612:1615	arg1	potential					1617:1625	good potential	1612:1625	good potential	1612:1625	The results indicate that MCP and its fractions have good potential as immunotherapeutic adjuvants.
25178064	11	58	theme	good	1612:1615	arg1	adjuvants					1648:1656	immunotherapeutic adjuvants	1630:1656	immunotherapeutic adjuvants	1630:1656	The results indicate that MCP and its fractions have good potential as immunotherapeutic adjuvants.
25178064	3	59	from	studies	205:211	arg1	composition					220:230	its composition	216:230	its composition	216:230	Until now, studies on its composition and related activities have been confined to compounds of low molecular mass, and no data have been reported concerning the plant's polysaccharides.
25178064	3	59	from	studies	205:211	arg1	activities					244:253	related activities	236:253	related activities	236:253	Until now, studies on its composition and related activities have been confined to compounds of low molecular mass, and no data have been reported concerning the plant's polysaccharides.
25178064	3	60	theme	mass	304:307	arg1	compounds					277:285	compounds	277:285	compounds of low molecular mass	277:307	Until now, studies on its composition and related activities have been confined to compounds of low molecular mass, and no data have been reported concerning the plant's polysaccharides.
25178064	6	61	theme	carbolic	836:843	arg1	index					864:868	the carbolic particle clearance index	832:868	the carbolic particle clearance index	832:868	The results showed that intragastric administration of 150 or 300 mg·kg-·d⁻¹ of MCP significantly increased the carbolic particle clearance index, serum haemolysin production, spleen index, thymus index and NK cell cytotoxicity to normal control levels in cyclophosphamide (Cy)-induced immunosuppressed mice.
25178064	6	62	theme	immunosuppressed	1010:1025	arg1	mice					1027:1030	cyclophosphamide (Cy)-induced immunosuppressed mice	980:1030	cyclophosphamide (Cy)-induced immunosuppressed mice	980:1030	The results showed that intragastric administration of 150 or 300 mg·kg-·d⁻¹ of MCP significantly increased the carbolic particle clearance index, serum haemolysin production, spleen index, thymus index and NK cell cytotoxicity to normal control levels in cyclophosphamide (Cy)-induced immunosuppressed mice.
25178064	0	63	theme	Momordica	79:87	arg1	charantia					89:97	Momordica charantia	79:97	Momordica charantia	79:97	Immunomodulatory activity and partial characterisation of polysaccharides from Momordica charantia.
25178064	2	64	theme	edible	140:145	arg1	vegetable					161:169	an edible and medicinal vegetable	137:169	an edible and medicinal vegetable in sub-tropical areas	137:191	is used as an edible and medicinal vegetable in sub-tropical areas.
25178064	11	65	theme	immunotherapeutic	1630:1646	arg1	potential					1617:1625	good potential	1612:1625	good potential	1612:1625	The results indicate that MCP and its fractions have good potential as immunotherapeutic adjuvants.
25178064	11	65	theme	immunotherapeutic	1630:1646	arg1	adjuvants					1648:1656	immunotherapeutic adjuvants	1630:1656	immunotherapeutic adjuvants	1630:1656	The results indicate that MCP and its fractions have good potential as immunotherapeutic adjuvants.
25178064	6	66	theme	-induced	1001:1008	arg1	mice					1027:1030	cyclophosphamide (Cy)-induced immunosuppressed mice	980:1030	cyclophosphamide (Cy)-induced immunosuppressed mice	980:1030	The results showed that intragastric administration of 150 or 300 mg·kg-·d⁻¹ of MCP significantly increased the carbolic particle clearance index, serum haemolysin production, spleen index, thymus index and NK cell cytotoxicity to normal control levels in cyclophosphamide (Cy)-induced immunosuppressed mice.
24315947	3	0	theme	reaction	326:333	arg1	efficiency					335:344	The reaction efficiency	322:344	The reaction efficiency of dry method esterified starch	322:376	The reaction efficiency of dry method esterified starch reached 92.34%.
24315947	5	1	theme	polylactic	737:746	arg1	acid					748:751	polylactic acid	737:751	polylactic acid	737:751	The degree of crystallinity of the ES/PLA was lower than that of the NS/PLA, indicating that the relative dependence between these two components of starch and polylactic acid was enhanced.
24315947	6	2	from	break	993:997	arg1	strength					947:954	the tensile strength	935:954	the tensile strength	935:954	Scanning electron microscopy (SEM) indicated that the dry method esterified starch increased the two-phase interface compatibility of the composites, thereby improving the tensile strength, bending strength, and elongation at break of the ES/PLA composite.
24315947	6	2	from	break	993:997	arg1	strength					965:972	bending strength	957:972	bending strength	957:972	Scanning electron microscopy (SEM) indicated that the dry method esterified starch increased the two-phase interface compatibility of the composites, thereby improving the tensile strength, bending strength, and elongation at break of the ES/PLA composite.
24315947	6	2	from	break	993:997	arg1	elongation					979:988	elongation	979:988	elongation	979:988	Scanning electron microscopy (SEM) indicated that the dry method esterified starch increased the two-phase interface compatibility of the composites, thereby improving the tensile strength, bending strength, and elongation at break of the ES/PLA composite.
24315947	9	3	theme	Strain	1308:1313	arg1	scanning					1315:1322	Strain scanning	1308:1322	Strain scanning	1308:1322	Strain scanning also showed that the compatibility of ES/PLA was increased.
24315947	7	4	from	introduction	1028:1039	arg1	compatibility					1095:1107	interface compatibility	1085:1107	interface compatibility	1085:1107	The introduction of a hydrophobic ester bond and increase in interface compatibility led to an increase in ES/PLA water resistance.
24315947	5	5	theme	acid	748:751	arg1	components					712:721	these two components	702:721	these two components of starch and polylactic acid	702:751	The degree of crystallinity of the ES/PLA was lower than that of the NS/PLA, indicating that the relative dependence between these two components of starch and polylactic acid was enhanced.
24315947	7	6	from	increase	1119:1126	arg1	resistance					1144:1153	ES/PLA water resistance	1131:1153	ES/PLA water resistance	1131:1153	The introduction of a hydrophobic ester bond and increase in interface compatibility led to an increase in ES/PLA water resistance.
24315947	6	7	theme	dry	821:823	arg1	method					825:830	the dry method	817:830	the dry method esterified starch	817:848	Scanning electron microscopy (SEM) indicated that the dry method esterified starch increased the two-phase interface compatibility of the composites, thereby improving the tensile strength, bending strength, and elongation at break of the ES/PLA composite.
24315947	3	8	theme	starch	371:376	arg1	efficiency					335:344	The reaction efficiency	322:344	The reaction efficiency of dry method esterified starch	322:376	The reaction efficiency of dry method esterified starch reached 92.34%.
24315947	10	9	theme	storage	1425:1431	arg1	modulus					1433:1439	storage modulus	1425:1439	storage modulus	1425:1439	While frequency scanning showed that the storage modulus and complex viscosity of ES/PLA was less than that of NS/PLA.
24315947	10	10	theme	complex	1445:1451	arg1	viscosity					1453:1461	complex viscosity	1445:1461	complex viscosity	1445:1461	While frequency scanning showed that the storage modulus and complex viscosity of ES/PLA was less than that of NS/PLA.
24315947	4	11	theme	dry	398:400	arg1	method					402:407	The dry method	394:407	The dry method esterified starch	394:425	The dry method esterified starch was blended with polylactic acid (PLA), and the mixture was melted and extruded to produce the esterified starch/polylactic acid (ES/PLA) composites.
24315947	2	12	theme	esterified	301:310	arg1	starches					312:319	the esterified starches	297:319	the esterified starches	297:319	Fourier transform infrared spectroscopy (FTIR) was used for the qualitative analysis of the esterified starches.
24315947	10	13	dep	modulus	1433:1439	arg1	the					1421:1423	the	1421:1423	the	1421:1423	While frequency scanning showed that the storage modulus and complex viscosity of ES/PLA was less than that of NS/PLA.
24315947	6	14	theme	interface	874:882	arg1	compatibility					884:896	the two-phase interface compatibility	860:896	the two-phase interface compatibility of the composites	860:914	Scanning electron microscopy (SEM) indicated that the dry method esterified starch increased the two-phase interface compatibility of the composites, thereby improving the tensile strength, bending strength, and elongation at break of the ES/PLA composite.
24315947	7	15	theme	interface	1085:1093	arg1	compatibility					1095:1107	interface compatibility	1085:1107	interface compatibility	1085:1107	The introduction of a hydrophobic ester bond and increase in interface compatibility led to an increase in ES/PLA water resistance.
24315947	5	16	theme	crystallinity	591:603	arg1	lower					623:627	lower	623:627	lower	623:627	The degree of crystallinity of the ES/PLA was lower than that of the NS/PLA, indicating that the relative dependence between these two components of starch and polylactic acid was enhanced.
24315947	5	16	theme	crystallinity	591:603	arg1	degree					581:586	The degree	577:586	The degree of crystallinity of the ES/PLA	577:617	The degree of crystallinity of the ES/PLA was lower than that of the NS/PLA, indicating that the relative dependence between these two components of starch and polylactic acid was enhanced.
24315947	3	17	theme	method	353:358	arg1	starch					371:376	dry method esterified starch	349:376	dry method esterified starch	349:376	The reaction efficiency of dry method esterified starch reached 92.34%.
24315947	6	18	theme	two-phase	864:872	arg1	compatibility					884:896	the two-phase interface compatibility	860:896	the two-phase interface compatibility of the composites	860:914	Scanning electron microscopy (SEM) indicated that the dry method esterified starch increased the two-phase interface compatibility of the composites, thereby improving the tensile strength, bending strength, and elongation at break of the ES/PLA composite.
24315947	6	19	theme	electron	776:783	arg1	microscopy					785:794	Scanning electron microscopy	767:794	Scanning electron microscopy (SEM)	767:800	Scanning electron microscopy (SEM) indicated that the dry method esterified starch increased the two-phase interface compatibility of the composites, thereby improving the tensile strength, bending strength, and elongation at break of the ES/PLA composite.
24315947	6	19	theme	electron	776:783	arg1	SEM					797:799	SEM	797:799	SEM	797:799	Scanning electron microscopy (SEM) indicated that the dry method esterified starch increased the two-phase interface compatibility of the composites, thereby improving the tensile strength, bending strength, and elongation at break of the ES/PLA composite.
24315947	2	20	used	used	260:263	arg2	Fourier					209:215	Fourier	209:215	Fourier transform infrared spectroscopy (FTIR)	209:254	Fourier transform infrared spectroscopy (FTIR) was used for the qualitative analysis of the esterified starches.
24315947	3	21	theme	esterified	360:369	arg1	starch					371:376	dry method esterified starch	349:376	dry method esterified starch	349:376	The reaction efficiency of dry method esterified starch reached 92.34%.
24315947	5	22	theme	ES/PLA	612:617	arg1	crystallinity					591:603	crystallinity	591:603	crystallinity of the ES/PLA	591:617	The degree of crystallinity of the ES/PLA was lower than that of the NS/PLA, indicating that the relative dependence between these two components of starch and polylactic acid was enhanced.
24315947	6	23	theme	Scanning	767:774	arg1	microscopy					785:794	Scanning electron microscopy	767:794	Scanning electron microscopy (SEM)	767:800	Scanning electron microscopy (SEM) indicated that the dry method esterified starch increased the two-phase interface compatibility of the composites, thereby improving the tensile strength, bending strength, and elongation at break of the ES/PLA composite.
24315947	6	23	theme	Scanning	767:774	arg1	SEM					797:799	SEM	797:799	SEM	797:799	Scanning electron microscopy (SEM) indicated that the dry method esterified starch increased the two-phase interface compatibility of the composites, thereby improving the tensile strength, bending strength, and elongation at break of the ES/PLA composite.
24315947	2	24	theme	qualitative	273:283	arg1	analysis					285:292	the qualitative analysis	269:292	the qualitative analysis of the esterified starches	269:319	Fourier transform infrared spectroscopy (FTIR) was used for the qualitative analysis of the esterified starches.
24315947	7	25	from	compatibility	1095:1107	arg1	introduction					1028:1039	The introduction	1024:1039	The introduction of a hydrophobic ester bond and increase in interface compatibility	1024:1107	The introduction of a hydrophobic ester bond and increase in interface compatibility led to an increase in ES/PLA water resistance.
24315947	8	26	theme	index	1161:1165	arg1	results					1181:1187	Melt index determination results	1156:1187	Melt index determination results	1156:1187	Melt index determination results showed that starch esterification modification had improved the melt flow properties of starch/PLA composite material.
24315947	7	27	from	increase	1073:1080	arg1	compatibility					1095:1107	interface compatibility	1085:1107	interface compatibility	1085:1107	The introduction of a hydrophobic ester bond and increase in interface compatibility led to an increase in ES/PLA water resistance.
24315947	7	28	theme	ester	1058:1062	arg1	bond					1064:1067	a hydrophobic ester bond	1044:1067	a hydrophobic ester bond	1044:1067	The introduction of a hydrophobic ester bond and increase in interface compatibility led to an increase in ES/PLA water resistance.
24315947	5	29	theme	starch	726:731	arg1	components					712:721	these two components	702:721	these two components of starch and polylactic acid	702:751	The degree of crystallinity of the ES/PLA was lower than that of the NS/PLA, indicating that the relative dependence between these two components of starch and polylactic acid was enhanced.
24315947	1	30	theme	Corn	102:105	arg1	starch					107:112	Corn starch	102:112	Corn starch	102:112	Corn starch and maleic anhydride were synthesized from a maleic anhydride esterified starch by dry method.
24315947	1	31	theme	dry	197:199	arg1	method					201:206	dry method	197:206	dry method	197:206	Corn starch and maleic anhydride were synthesized from a maleic anhydride esterified starch by dry method.
24315947	8	32	theme	Melt	1156:1159	arg1	results					1181:1187	Melt index determination results	1156:1187	Melt index determination results	1156:1187	Melt index determination results showed that starch esterification modification had improved the melt flow properties of starch/PLA composite material.
24315947	4	33	theme	acid	551:554	arg1	composites					565:574	the esterified starch/polylactic acid (ES/PLA) composites	518:574	the esterified starch/polylactic acid (ES/PLA) composites	518:574	The dry method esterified starch was blended with polylactic acid (PLA), and the mixture was melted and extruded to produce the esterified starch/polylactic acid (ES/PLA) composites.
24315947	7	34	theme	bond	1064:1067	arg1	introduction					1028:1039	The introduction	1024:1039	The introduction of a hydrophobic ester bond and increase in interface compatibility	1024:1107	The introduction of a hydrophobic ester bond and increase in interface compatibility led to an increase in ES/PLA water resistance.
24315947	1	35	dep	esterified	176:185	arg1	starch					187:192	starch	187:192	starch	187:192	Corn starch and maleic anhydride were synthesized from a maleic anhydride esterified starch by dry method.
24315947	1	35	dep	esterified	176:185	arg1	anhydride					166:174	a maleic anhydride	157:174	a maleic anhydride esterified starch by dry method	157:206	Corn starch and maleic anhydride were synthesized from a maleic anhydride esterified starch by dry method.
24315947	7	36	theme	ES/PLA	1131:1136	arg1	resistance					1144:1153	ES/PLA water resistance	1131:1153	ES/PLA water resistance	1131:1153	The introduction of a hydrophobic ester bond and increase in interface compatibility led to an increase in ES/PLA water resistance.
24315947	4	37	theme	starch/polylactic	533:549	arg1	acid					551:554	the esterified starch/polylactic acid	518:554	the esterified starch/polylactic acid (ES/PLA) composites	518:574	The dry method esterified starch was blended with polylactic acid (PLA), and the mixture was melted and extruded to produce the esterified starch/polylactic acid (ES/PLA) composites.
24315947	4	37	theme	starch/polylactic	533:549	arg1	ES/PLA					557:562	ES/PLA	557:562	ES/PLA	557:562	The dry method esterified starch was blended with polylactic acid (PLA), and the mixture was melted and extruded to produce the esterified starch/polylactic acid (ES/PLA) composites.
24315947	2	38	theme	starches	312:319	arg1	analysis					285:292	the qualitative analysis	269:292	the qualitative analysis of the esterified starches	269:319	Fourier transform infrared spectroscopy (FTIR) was used for the qualitative analysis of the esterified starches.
24315947	10	39	theme	ES/PLA	1466:1471	arg1	viscosity					1453:1461	complex viscosity	1445:1461	complex viscosity	1445:1461	While frequency scanning showed that the storage modulus and complex viscosity of ES/PLA was less than that of NS/PLA.
24315947	10	39	theme	ES/PLA	1466:1471	arg1	modulus					1433:1439	storage modulus	1425:1439	storage modulus	1425:1439	While frequency scanning showed that the storage modulus and complex viscosity of ES/PLA was less than that of NS/PLA.
24315947	8	40	theme	flow	1258:1261	arg1	properties					1263:1272	the melt flow properties	1249:1272	the melt flow properties of starch/PLA composite material	1249:1305	Melt index determination results showed that starch esterification modification had improved the melt flow properties of starch/PLA composite material.
24315947	7	41	theme	water	1138:1142	arg1	resistance					1144:1153	ES/PLA water resistance	1131:1153	ES/PLA water resistance	1131:1153	The introduction of a hydrophobic ester bond and increase in interface compatibility led to an increase in ES/PLA water resistance.
24315947	5	42	theme	relative	674:681	arg1	dependence					683:692	the relative dependence	670:692	the relative dependence between these two components of starch and polylactic acid	670:751	The degree of crystallinity of the ES/PLA was lower than that of the NS/PLA, indicating that the relative dependence between these two components of starch and polylactic acid was enhanced.
24315947	1	43	theme	maleic	118:123	arg1	anhydride					125:133	maleic anhydride	118:133	maleic anhydride	118:133	Corn starch and maleic anhydride were synthesized from a maleic anhydride esterified starch by dry method.
24315947	8	44	theme	melt	1253:1256	arg1	properties					1263:1272	the melt flow properties	1249:1272	the melt flow properties of starch/PLA composite material	1249:1305	Melt index determination results showed that starch esterification modification had improved the melt flow properties of starch/PLA composite material.
24315947	0	45	theme	method	40:45	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of dry method esterified starch/polylactic acid composite materials.
24315947	0	45	theme	method	40:45	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of dry method esterified starch/polylactic acid composite materials.
24315947	6	46	theme	tensile	939:945	arg1	strength					947:954	the tensile strength	935:954	the tensile strength	935:954	Scanning electron microscopy (SEM) indicated that the dry method esterified starch increased the two-phase interface compatibility of the composites, thereby improving the tensile strength, bending strength, and elongation at break of the ES/PLA composite.
24315947	6	47	theme	esterified	832:841	arg1	starch					843:848	the dry method esterified starch	817:848	the dry method esterified starch	817:848	Scanning electron microscopy (SEM) indicated that the dry method esterified starch increased the two-phase interface compatibility of the composites, thereby improving the tensile strength, bending strength, and elongation at break of the ES/PLA composite.
24315947	8	48	theme	determination	1167:1179	arg1	results					1181:1187	Melt index determination results	1156:1187	Melt index determination results	1156:1187	Melt index determination results showed that starch esterification modification had improved the melt flow properties of starch/PLA composite material.
24315947	6	49	theme	composite	1013:1021	arg1	strength					947:954	the tensile strength	935:954	the tensile strength	935:954	Scanning electron microscopy (SEM) indicated that the dry method esterified starch increased the two-phase interface compatibility of the composites, thereby improving the tensile strength, bending strength, and elongation at break of the ES/PLA composite.
24315947	6	49	theme	composite	1013:1021	arg1	strength					965:972	bending strength	957:972	bending strength	957:972	Scanning electron microscopy (SEM) indicated that the dry method esterified starch increased the two-phase interface compatibility of the composites, thereby improving the tensile strength, bending strength, and elongation at break of the ES/PLA composite.
24315947	6	49	theme	composite	1013:1021	arg1	elongation					979:988	elongation	979:988	elongation	979:988	Scanning electron microscopy (SEM) indicated that the dry method esterified starch increased the two-phase interface compatibility of the composites, thereby improving the tensile strength, bending strength, and elongation at break of the ES/PLA composite.
24315947	0	50	theme	dry	36:38	arg1	method					40:45	dry method	36:45	dry method	36:45	Preparation and characterization of dry method esterified starch/polylactic acid composite materials.
24315947	9	51	theme	ES/PLA	1362:1367	arg1	compatibility					1345:1357	the compatibility	1341:1357	the compatibility of ES/PLA	1341:1367	Strain scanning also showed that the compatibility of ES/PLA was increased.
24315947	6	52	theme	method	825:830	arg1	starch					843:848	the dry method esterified starch	817:848	the dry method esterified starch	817:848	Scanning electron microscopy (SEM) indicated that the dry method esterified starch increased the two-phase interface compatibility of the composites, thereby improving the tensile strength, bending strength, and elongation at break of the ES/PLA composite.
24315947	6	53	theme	ES/PLA	1006:1011	arg1	composite					1013:1021	the ES/PLA composite	1002:1021	the ES/PLA composite	1002:1021	Scanning electron microscopy (SEM) indicated that the dry method esterified starch increased the two-phase interface compatibility of the composites, thereby improving the tensile strength, bending strength, and elongation at break of the ES/PLA composite.
24315947	0	54	theme	starch/polylactic	58:74	arg1	materials					91:99	starch/polylactic acid composite materials	58:99	starch/polylactic acid composite materials	58:99	Preparation and characterization of dry method esterified starch/polylactic acid composite materials.
24315947	0	55	theme	acid	76:79	arg1	materials					91:99	starch/polylactic acid composite materials	58:99	starch/polylactic acid composite materials	58:99	Preparation and characterization of dry method esterified starch/polylactic acid composite materials.
24315947	8	56	theme	composite	1288:1296	arg1	material					1298:1305	starch/PLA composite material	1277:1305	starch/PLA composite material	1277:1305	Melt index determination results showed that starch esterification modification had improved the melt flow properties of starch/PLA composite material.
24315947	8	57	theme	starch	1201:1206	arg1	modification					1223:1234	starch esterification modification	1201:1234	starch esterification modification	1201:1234	Melt index determination results showed that starch esterification modification had improved the melt flow properties of starch/PLA composite material.
24315947	4	58	theme	esterified	522:531	arg1	acid					551:554	the esterified starch/polylactic acid	518:554	the esterified starch/polylactic acid (ES/PLA) composites	518:574	The dry method esterified starch was blended with polylactic acid (PLA), and the mixture was melted and extruded to produce the esterified starch/polylactic acid (ES/PLA) composites.
24315947	4	58	theme	esterified	522:531	arg1	ES/PLA					557:562	ES/PLA	557:562	ES/PLA	557:562	The dry method esterified starch was blended with polylactic acid (PLA), and the mixture was melted and extruded to produce the esterified starch/polylactic acid (ES/PLA) composites.
24315947	7	59	theme	increase	1073:1080	arg1	introduction					1028:1039	The introduction	1024:1039	The introduction of a hydrophobic ester bond and increase in interface compatibility	1024:1107	The introduction of a hydrophobic ester bond and increase in interface compatibility led to an increase in ES/PLA water resistance.
24315947	8	60	theme	starch/PLA	1277:1286	arg1	material					1298:1305	starch/PLA composite material	1277:1305	starch/PLA composite material	1277:1305	Melt index determination results showed that starch esterification modification had improved the melt flow properties of starch/PLA composite material.
24315947	2	61	dep	Fourier	209:215	arg1	transform					217:225	transform	217:225	transform infrared spectroscopy (FTIR)	217:254	Fourier transform infrared spectroscopy (FTIR) was used for the qualitative analysis of the esterified starches.
24315947	7	62	theme	hydrophobic	1046:1056	arg1	bond					1064:1067	a hydrophobic ester bond	1044:1067	a hydrophobic ester bond	1044:1067	The introduction of a hydrophobic ester bond and increase in interface compatibility led to an increase in ES/PLA water resistance.
24315947	4	63	theme	polylactic	444:453	arg1	PLA					461:463	PLA	461:463	PLA	461:463	The dry method esterified starch was blended with polylactic acid (PLA), and the mixture was melted and extruded to produce the esterified starch/polylactic acid (ES/PLA) composites.
24315947	4	63	theme	polylactic	444:453	arg1	acid					455:458	polylactic acid	444:458	polylactic acid (PLA)	444:464	The dry method esterified starch was blended with polylactic acid (PLA), and the mixture was melted and extruded to produce the esterified starch/polylactic acid (ES/PLA) composites.
24315947	7	64	from	bond	1064:1067	arg1	compatibility					1095:1107	interface compatibility	1085:1107	interface compatibility	1085:1107	The introduction of a hydrophobic ester bond and increase in interface compatibility led to an increase in ES/PLA water resistance.
24315947	8	65	theme	material	1298:1305	arg1	properties					1263:1272	the melt flow properties	1249:1272	the melt flow properties of starch/PLA composite material	1249:1305	Melt index determination results showed that starch esterification modification had improved the melt flow properties of starch/PLA composite material.
24315947	8	66	theme	esterification	1208:1221	arg1	modification					1223:1234	starch esterification modification	1201:1234	starch esterification modification	1201:1234	Melt index determination results showed that starch esterification modification had improved the melt flow properties of starch/PLA composite material.
24315947	0	67	theme	composite	81:89	arg1	materials					91:99	starch/polylactic acid composite materials	58:99	starch/polylactic acid composite materials	58:99	Preparation and characterization of dry method esterified starch/polylactic acid composite materials.
24315947	10	68	theme	frequency	1390:1398	arg1	scanning					1400:1407	frequency scanning	1390:1407	frequency scanning	1390:1407	While frequency scanning showed that the storage modulus and complex viscosity of ES/PLA was less than that of NS/PLA.
24315947	3	69	theme	dry	349:351	arg1	starch					371:376	dry method esterified starch	349:376	dry method esterified starch	349:376	The reaction efficiency of dry method esterified starch reached 92.34%.
24315947	6	70	theme	composites	905:914	arg1	compatibility					884:896	the two-phase interface compatibility	860:896	the two-phase interface compatibility of the composites	860:914	Scanning electron microscopy (SEM) indicated that the dry method esterified starch increased the two-phase interface compatibility of the composites, thereby improving the tensile strength, bending strength, and elongation at break of the ES/PLA composite.
24315947	6	71	theme	bending	957:963	arg1	strength					965:972	bending strength	957:972	bending strength	957:972	Scanning electron microscopy (SEM) indicated that the dry method esterified starch increased the two-phase interface compatibility of the composites, thereby improving the tensile strength, bending strength, and elongation at break of the ES/PLA composite.
24315947	1	72	theme	maleic	159:164	arg1	starch					187:192	starch	187:192	starch	187:192	Corn starch and maleic anhydride were synthesized from a maleic anhydride esterified starch by dry method.
24315947	1	72	theme	maleic	159:164	arg1	anhydride					166:174	a maleic anhydride	157:174	a maleic anhydride esterified starch by dry method	157:206	Corn starch and maleic anhydride were synthesized from a maleic anhydride esterified starch by dry method.
24315947	2	73	dep	transform	217:225	arg1	infrared					227:234	infrared	227:234	transform infrared spectroscopy (FTIR)	217:254	Fourier transform infrared spectroscopy (FTIR) was used for the qualitative analysis of the esterified starches.
25263928	2	0	theme	different	610:618	arg1	degrees					620:626	different degrees	610:626	different degrees of methyl-esterification	610:651	Based on the data from monosaccharide composition and (13)C NMR analysis, GPW-1, GPR-1 and GPS-1 were identified as type-I rhamnogalacturonan (RG-I)-rich pectins, GPW-2, GPR-2 and GPS-2 were homogalacturonan (HG)-rich pectins with different degrees of methyl-esterification.
25263928	4	1	theme	diabetic	991:998	arg1	mice					1000:1003	alloxan-induced diabetic mice	975:1003	alloxan-induced diabetic mice	975:1003	In vivo animal experiments showed that GPs exhibited significant antiohyperglycemic and antioxidant activities in alloxan-induced diabetic mice, and the effects increased with the processing temperature, with the most potent activity in GPS.
25263928	0	2	theme	heat	77:80	arg1	processing					82:91	heat processing	77:91	heat processing	77:91	Chemical and antihyperglycemic activity changes of ginseng pectin induced by heat processing.
25263928	4	3	theme	In	861:862	arg1	experiments					876:886	In vivo animal experiments	861:886	In vivo animal experiments	861:886	In vivo animal experiments showed that GPs exhibited significant antiohyperglycemic and antioxidant activities in alloxan-induced diabetic mice, and the effects increased with the processing temperature, with the most potent activity in GPS.
25263928	1	4	dep	ginseng	241:247	arg1	GPS-2					260:264	GPS-2	260:264	GPS-2	260:264	Six pectic polysaccharides were obtained from white ginseng (GPW-1 and GPW-2), red ginseng (GPR-1 and GPR-2, steamed ginseng at 100°C) and steamed ginseng (GPS-1 and GPS-2, steamed ginseng at 120°C) by combination of water extraction, ion-exchange and gel permeation chromatographies.
25263928	1	4	dep	ginseng	241:247	arg1	ginseng					275:281	steamed ginseng	267:281	steamed ginseng at 120°C	267:290	Six pectic polysaccharides were obtained from white ginseng (GPW-1 and GPW-2), red ginseng (GPR-1 and GPR-2, steamed ginseng at 100°C) and steamed ginseng (GPS-1 and GPS-2, steamed ginseng at 120°C) by combination of water extraction, ion-exchange and gel permeation chromatographies.
25263928	1	4	dep	ginseng	241:247	arg1	GPS-1					250:254	GPS-1	250:254	GPS-1	250:254	Six pectic polysaccharides were obtained from white ginseng (GPW-1 and GPW-2), red ginseng (GPR-1 and GPR-2, steamed ginseng at 100°C) and steamed ginseng (GPS-1 and GPS-2, steamed ginseng at 120°C) by combination of water extraction, ion-exchange and gel permeation chromatographies.
25263928	3	5	from	increase	690:697	arg1	fractions					739:747	these six fractions	729:747	these six fractions	729:747	Remarkably, GalA increased with the increase of processing temperatures in these six fractions, which might be caused by the transformation of esterified GalA into un-esterified form during heat processing.
25263928	4	6	theme	animal	869:874	arg1	experiments					876:886	In vivo animal experiments	861:886	In vivo animal experiments	861:886	In vivo animal experiments showed that GPs exhibited significant antiohyperglycemic and antioxidant activities in alloxan-induced diabetic mice, and the effects increased with the processing temperature, with the most potent activity in GPS.
25263928	3	7	theme	un-esterified	818:830	arg1	form					832:835	un-esterified form	818:835	un-esterified form during heat processing	818:858	Remarkably, GalA increased with the increase of processing temperatures in these six fractions, which might be caused by the transformation of esterified GalA into un-esterified form during heat processing.
25263928	2	8	theme	NMR	439:441	arg1	analysis					443:450	(13)C NMR analysis	433:450	(13)C NMR analysis	433:450	Based on the data from monosaccharide composition and (13)C NMR analysis, GPW-1, GPR-1 and GPS-1 were identified as type-I rhamnogalacturonan (RG-I)-rich pectins, GPW-2, GPR-2 and GPS-2 were homogalacturonan (HG)-rich pectins with different degrees of methyl-esterification.
25263928	4	9	theme	significant	914:924	arg1	activities					961:970	significant antiohyperglycemic and antioxidant activities	914:970	significant antiohyperglycemic and antioxidant activities	914:970	In vivo animal experiments showed that GPs exhibited significant antiohyperglycemic and antioxidant activities in alloxan-induced diabetic mice, and the effects increased with the processing temperature, with the most potent activity in GPS.
25263928	4	10	theme	antioxidant	949:959	arg1	activities					961:970	significant antiohyperglycemic and antioxidant activities	914:970	significant antiohyperglycemic and antioxidant activities	914:970	In vivo animal experiments showed that GPs exhibited significant antiohyperglycemic and antioxidant activities in alloxan-induced diabetic mice, and the effects increased with the processing temperature, with the most potent activity in GPS.
25263928	3	11	theme	esterified	797:806	arg1	GalA					808:811	esterified GalA	797:811	esterified GalA	797:811	Remarkably, GalA increased with the increase of processing temperatures in these six fractions, which might be caused by the transformation of esterified GalA into un-esterified form during heat processing.
25263928	1	12	theme	water	311:315	arg1	extraction					317:326	water extraction	311:326	water extraction	311:326	Six pectic polysaccharides were obtained from white ginseng (GPW-1 and GPW-2), red ginseng (GPR-1 and GPR-2, steamed ginseng at 100°C) and steamed ginseng (GPS-1 and GPS-2, steamed ginseng at 120°C) by combination of water extraction, ion-exchange and gel permeation chromatographies.
25263928	3	13	theme	GalA	808:811	arg1	transformation					779:792	the transformation	775:792	the transformation of esterified GalA into un-esterified form during heat processing	775:858	Remarkably, GalA increased with the increase of processing temperatures in these six fractions, which might be caused by the transformation of esterified GalA into un-esterified form during heat processing.
25263928	0	14	theme	Chemical	0:7	arg1	changes					40:46	Chemical and antihyperglycemic activity changes	0:46	Chemical and antihyperglycemic activity changes of ginseng pectin	0:64	Chemical and antihyperglycemic activity changes of ginseng pectin induced by heat processing.
25263928	1	15	theme	steamed	233:239	arg1	ginseng					241:247	steamed ginseng	233:247	steamed ginseng (GPS-1 and GPS-2, steamed ginseng at 120°C)	233:291	Six pectic polysaccharides were obtained from white ginseng (GPW-1 and GPW-2), red ginseng (GPR-1 and GPR-2, steamed ginseng at 100°C) and steamed ginseng (GPS-1 and GPS-2, steamed ginseng at 120°C) by combination of water extraction, ion-exchange and gel permeation chromatographies.
25263928	2	16	theme	-rich	527:531	arg1	GPR-2					549:553	GPR-2	549:553	GPR-2	549:553	Based on the data from monosaccharide composition and (13)C NMR analysis, GPW-1, GPR-1 and GPS-1 were identified as type-I rhamnogalacturonan (RG-I)-rich pectins, GPW-2, GPR-2 and GPS-2 were homogalacturonan (HG)-rich pectins with different degrees of methyl-esterification.
25263928	2	16	theme	-rich	527:531	arg1	GPW-1					453:457	GPW-1	453:457	GPW-1	453:457	Based on the data from monosaccharide composition and (13)C NMR analysis, GPW-1, GPR-1 and GPS-1 were identified as type-I rhamnogalacturonan (RG-I)-rich pectins, GPW-2, GPR-2 and GPS-2 were homogalacturonan (HG)-rich pectins with different degrees of methyl-esterification.
25263928	2	16	theme	-rich	527:531	arg1	GPS-2					559:563	GPS-2	559:563	GPS-2	559:563	Based on the data from monosaccharide composition and (13)C NMR analysis, GPW-1, GPR-1 and GPS-1 were identified as type-I rhamnogalacturonan (RG-I)-rich pectins, GPW-2, GPR-2 and GPS-2 were homogalacturonan (HG)-rich pectins with different degrees of methyl-esterification.
25263928	2	16	theme	-rich	527:531	arg1	pectins					533:539	type-I rhamnogalacturonan (RG-I)-rich pectins	495:539	type-I rhamnogalacturonan (RG-I)-rich pectins	495:539	Based on the data from monosaccharide composition and (13)C NMR analysis, GPW-1, GPR-1 and GPS-1 were identified as type-I rhamnogalacturonan (RG-I)-rich pectins, GPW-2, GPR-2 and GPS-2 were homogalacturonan (HG)-rich pectins with different degrees of methyl-esterification.
25263928	2	16	theme	-rich	527:531	arg1	GPR-1					460:464	GPR-1	460:464	GPR-1	460:464	Based on the data from monosaccharide composition and (13)C NMR analysis, GPW-1, GPR-1 and GPS-1 were identified as type-I rhamnogalacturonan (RG-I)-rich pectins, GPW-2, GPR-2 and GPS-2 were homogalacturonan (HG)-rich pectins with different degrees of methyl-esterification.
25263928	2	16	theme	-rich	527:531	arg1	GPW-2					542:546	GPW-2	542:546	GPW-2	542:546	Based on the data from monosaccharide composition and (13)C NMR analysis, GPW-1, GPR-1 and GPS-1 were identified as type-I rhamnogalacturonan (RG-I)-rich pectins, GPW-2, GPR-2 and GPS-2 were homogalacturonan (HG)-rich pectins with different degrees of methyl-esterification.
25263928	2	16	theme	-rich	527:531	arg1	GPS-1					470:474	GPS-1	470:474	GPS-1	470:474	Based on the data from monosaccharide composition and (13)C NMR analysis, GPW-1, GPR-1 and GPS-1 were identified as type-I rhamnogalacturonan (RG-I)-rich pectins, GPW-2, GPR-2 and GPS-2 were homogalacturonan (HG)-rich pectins with different degrees of methyl-esterification.
25263928	2	17	theme	homogalacturonan	570:585	arg1	pectins					597:603	homogalacturonan (HG)-rich pectins	570:603	homogalacturonan (HG)-rich pectins	570:603	Based on the data from monosaccharide composition and (13)C NMR analysis, GPW-1, GPR-1 and GPS-1 were identified as type-I rhamnogalacturonan (RG-I)-rich pectins, GPW-2, GPR-2 and GPS-2 were homogalacturonan (HG)-rich pectins with different degrees of methyl-esterification.
25263928	2	18	from	analysis	443:450	arg1	data					392:395	the data	388:395	the data from monosaccharide composition and (13)C NMR analysis	388:450	Based on the data from monosaccharide composition and (13)C NMR analysis, GPW-1, GPR-1 and GPS-1 were identified as type-I rhamnogalacturonan (RG-I)-rich pectins, GPW-2, GPR-2 and GPS-2 were homogalacturonan (HG)-rich pectins with different degrees of methyl-esterification.
25263928	3	19	theme	heat	844:847	arg1	processing					849:858	heat processing	844:858	heat processing	844:858	Remarkably, GalA increased with the increase of processing temperatures in these six fractions, which might be caused by the transformation of esterified GalA into un-esterified form during heat processing.
25263928	1	20	theme	red	173:175	arg1	ginseng					177:183	red ginseng	173:183	red ginseng (GPR-1 and GPR-2, steamed ginseng at 100°C)	173:227	Six pectic polysaccharides were obtained from white ginseng (GPW-1 and GPW-2), red ginseng (GPR-1 and GPR-2, steamed ginseng at 100°C) and steamed ginseng (GPS-1 and GPS-2, steamed ginseng at 120°C) by combination of water extraction, ion-exchange and gel permeation chromatographies.
25263928	0	21	theme	activity	31:38	arg1	changes					40:46	Chemical and antihyperglycemic activity changes	0:46	Chemical and antihyperglycemic activity changes of ginseng pectin	0:64	Chemical and antihyperglycemic activity changes of ginseng pectin induced by heat processing.
25263928	1	22	dep	ginseng	146:152	arg1	GPW-2					165:169	GPW-2	165:169	GPW-2	165:169	Six pectic polysaccharides were obtained from white ginseng (GPW-1 and GPW-2), red ginseng (GPR-1 and GPR-2, steamed ginseng at 100°C) and steamed ginseng (GPS-1 and GPS-2, steamed ginseng at 120°C) by combination of water extraction, ion-exchange and gel permeation chromatographies.
25263928	1	22	dep	ginseng	146:152	arg1	GPW-1					155:159	GPW-1	155:159	GPW-1	155:159	Six pectic polysaccharides were obtained from white ginseng (GPW-1 and GPW-2), red ginseng (GPR-1 and GPR-2, steamed ginseng at 100°C) and steamed ginseng (GPS-1 and GPS-2, steamed ginseng at 120°C) by combination of water extraction, ion-exchange and gel permeation chromatographies.
25263928	0	23	theme	antihyperglycemic	13:29	arg1	changes					40:46	Chemical and antihyperglycemic activity changes	0:46	Chemical and antihyperglycemic activity changes of ginseng pectin	0:64	Chemical and antihyperglycemic activity changes of ginseng pectin induced by heat processing.
25263928	1	24	theme	extraction	317:326	arg1	combination					296:306	combination	296:306	combination of water extraction, ion-exchange and gel permeation chromatographies	296:376	Six pectic polysaccharides were obtained from white ginseng (GPW-1 and GPW-2), red ginseng (GPR-1 and GPR-2, steamed ginseng at 100°C) and steamed ginseng (GPS-1 and GPS-2, steamed ginseng at 120°C) by combination of water extraction, ion-exchange and gel permeation chromatographies.
25263928	4	25	theme	processing	1041:1050	arg1	temperature					1052:1062	the processing temperature	1037:1062	the processing temperature	1037:1062	In vivo animal experiments showed that GPs exhibited significant antiohyperglycemic and antioxidant activities in alloxan-induced diabetic mice, and the effects increased with the processing temperature, with the most potent activity in GPS.
25263928	1	26	from	120°C	286:290	arg1	ginseng					275:281	steamed ginseng	267:281	steamed ginseng at 120°C	267:290	Six pectic polysaccharides were obtained from white ginseng (GPW-1 and GPW-2), red ginseng (GPR-1 and GPR-2, steamed ginseng at 100°C) and steamed ginseng (GPS-1 and GPS-2, steamed ginseng at 120°C) by combination of water extraction, ion-exchange and gel permeation chromatographies.
25263928	1	26	from	120°C	286:290	arg1	GPS-1					250:254	GPS-1	250:254	GPS-1	250:254	Six pectic polysaccharides were obtained from white ginseng (GPW-1 and GPW-2), red ginseng (GPR-1 and GPR-2, steamed ginseng at 100°C) and steamed ginseng (GPS-1 and GPS-2, steamed ginseng at 120°C) by combination of water extraction, ion-exchange and gel permeation chromatographies.
25263928	4	27	from	activity	1086:1093	arg1	GPS					1098:1100	GPS	1098:1100	GPS	1098:1100	In vivo animal experiments showed that GPs exhibited significant antiohyperglycemic and antioxidant activities in alloxan-induced diabetic mice, and the effects increased with the processing temperature, with the most potent activity in GPS.
25263928	3	28	from	fractions	739:747	arg1	increase					690:697	the increase	686:697	the increase	686:697	Remarkably, GalA increased with the increase of processing temperatures in these six fractions, which might be caused by the transformation of esterified GalA into un-esterified form during heat processing.
25263928	4	29	theme	antiohyperglycemic	926:943	arg1	activities					961:970	significant antiohyperglycemic and antioxidant activities	914:970	significant antiohyperglycemic and antioxidant activities	914:970	In vivo animal experiments showed that GPs exhibited significant antiohyperglycemic and antioxidant activities in alloxan-induced diabetic mice, and the effects increased with the processing temperature, with the most potent activity in GPS.
25263928	1	30	from	100°C	222:226	arg1	ginseng					211:217	steamed ginseng	203:217	steamed ginseng at 100°C	203:226	Six pectic polysaccharides were obtained from white ginseng (GPW-1 and GPW-2), red ginseng (GPR-1 and GPR-2, steamed ginseng at 100°C) and steamed ginseng (GPS-1 and GPS-2, steamed ginseng at 120°C) by combination of water extraction, ion-exchange and gel permeation chromatographies.
25263928	1	30	from	100°C	222:226	arg1	GPR-1					186:190	GPR-1	186:190	GPR-1	186:190	Six pectic polysaccharides were obtained from white ginseng (GPW-1 and GPW-2), red ginseng (GPR-1 and GPR-2, steamed ginseng at 100°C) and steamed ginseng (GPS-1 and GPS-2, steamed ginseng at 120°C) by combination of water extraction, ion-exchange and gel permeation chromatographies.
25263928	1	31	theme	ion-exchange	329:340	arg1	combination					296:306	combination	296:306	combination of water extraction, ion-exchange and gel permeation chromatographies	296:376	Six pectic polysaccharides were obtained from white ginseng (GPW-1 and GPW-2), red ginseng (GPR-1 and GPR-2, steamed ginseng at 100°C) and steamed ginseng (GPS-1 and GPS-2, steamed ginseng at 120°C) by combination of water extraction, ion-exchange and gel permeation chromatographies.
25263928	4	32	theme	alloxan-induced	975:989	arg1	mice					1000:1003	alloxan-induced diabetic mice	975:1003	alloxan-induced diabetic mice	975:1003	In vivo animal experiments showed that GPs exhibited significant antiohyperglycemic and antioxidant activities in alloxan-induced diabetic mice, and the effects increased with the processing temperature, with the most potent activity in GPS.
25263928	0	33	theme	pectin	59:64	arg1	changes					40:46	Chemical and antihyperglycemic activity changes	0:46	Chemical and antihyperglycemic activity changes of ginseng pectin	0:64	Chemical and antihyperglycemic activity changes of ginseng pectin induced by heat processing.
25263928	2	34	theme	type-I	495:500	arg1	GPR-2					549:553	GPR-2	549:553	GPR-2	549:553	Based on the data from monosaccharide composition and (13)C NMR analysis, GPW-1, GPR-1 and GPS-1 were identified as type-I rhamnogalacturonan (RG-I)-rich pectins, GPW-2, GPR-2 and GPS-2 were homogalacturonan (HG)-rich pectins with different degrees of methyl-esterification.
25263928	2	34	theme	type-I	495:500	arg1	GPW-1					453:457	GPW-1	453:457	GPW-1	453:457	Based on the data from monosaccharide composition and (13)C NMR analysis, GPW-1, GPR-1 and GPS-1 were identified as type-I rhamnogalacturonan (RG-I)-rich pectins, GPW-2, GPR-2 and GPS-2 were homogalacturonan (HG)-rich pectins with different degrees of methyl-esterification.
25263928	2	34	theme	type-I	495:500	arg1	GPS-2					559:563	GPS-2	559:563	GPS-2	559:563	Based on the data from monosaccharide composition and (13)C NMR analysis, GPW-1, GPR-1 and GPS-1 were identified as type-I rhamnogalacturonan (RG-I)-rich pectins, GPW-2, GPR-2 and GPS-2 were homogalacturonan (HG)-rich pectins with different degrees of methyl-esterification.
25263928	2	34	theme	type-I	495:500	arg1	pectins					533:539	type-I rhamnogalacturonan (RG-I)-rich pectins	495:539	type-I rhamnogalacturonan (RG-I)-rich pectins	495:539	Based on the data from monosaccharide composition and (13)C NMR analysis, GPW-1, GPR-1 and GPS-1 were identified as type-I rhamnogalacturonan (RG-I)-rich pectins, GPW-2, GPR-2 and GPS-2 were homogalacturonan (HG)-rich pectins with different degrees of methyl-esterification.
25263928	2	34	theme	type-I	495:500	arg1	GPR-1					460:464	GPR-1	460:464	GPR-1	460:464	Based on the data from monosaccharide composition and (13)C NMR analysis, GPW-1, GPR-1 and GPS-1 were identified as type-I rhamnogalacturonan (RG-I)-rich pectins, GPW-2, GPR-2 and GPS-2 were homogalacturonan (HG)-rich pectins with different degrees of methyl-esterification.
25263928	2	34	theme	type-I	495:500	arg1	GPW-2					542:546	GPW-2	542:546	GPW-2	542:546	Based on the data from monosaccharide composition and (13)C NMR analysis, GPW-1, GPR-1 and GPS-1 were identified as type-I rhamnogalacturonan (RG-I)-rich pectins, GPW-2, GPR-2 and GPS-2 were homogalacturonan (HG)-rich pectins with different degrees of methyl-esterification.
25263928	2	34	theme	type-I	495:500	arg1	GPS-1					470:474	GPS-1	470:474	GPS-1	470:474	Based on the data from monosaccharide composition and (13)C NMR analysis, GPW-1, GPR-1 and GPS-1 were identified as type-I rhamnogalacturonan (RG-I)-rich pectins, GPW-2, GPR-2 and GPS-2 were homogalacturonan (HG)-rich pectins with different degrees of methyl-esterification.
25263928	0	35	theme	ginseng	51:57	arg1	pectin					59:64	ginseng pectin	51:64	ginseng pectin	51:64	Chemical and antihyperglycemic activity changes of ginseng pectin induced by heat processing.
25263928	1	36	theme	gel	346:348	arg1	chromatographies					361:376	gel permeation chromatographies	346:376	gel permeation chromatographies	346:376	Six pectic polysaccharides were obtained from white ginseng (GPW-1 and GPW-2), red ginseng (GPR-1 and GPR-2, steamed ginseng at 100°C) and steamed ginseng (GPS-1 and GPS-2, steamed ginseng at 120°C) by combination of water extraction, ion-exchange and gel permeation chromatographies.
25263928	2	37	theme	-rich	591:595	arg1	pectins					597:603	homogalacturonan (HG)-rich pectins	570:603	homogalacturonan (HG)-rich pectins	570:603	Based on the data from monosaccharide composition and (13)C NMR analysis, GPW-1, GPR-1 and GPS-1 were identified as type-I rhamnogalacturonan (RG-I)-rich pectins, GPW-2, GPR-2 and GPS-2 were homogalacturonan (HG)-rich pectins with different degrees of methyl-esterification.
25263928	1	38	theme	steamed	267:273	arg1	ginseng					275:281	steamed ginseng	267:281	steamed ginseng at 120°C	267:290	Six pectic polysaccharides were obtained from white ginseng (GPW-1 and GPW-2), red ginseng (GPR-1 and GPR-2, steamed ginseng at 100°C) and steamed ginseng (GPS-1 and GPS-2, steamed ginseng at 120°C) by combination of water extraction, ion-exchange and gel permeation chromatographies.
25263928	1	38	theme	steamed	267:273	arg1	GPS-1					250:254	GPS-1	250:254	GPS-1	250:254	Six pectic polysaccharides were obtained from white ginseng (GPW-1 and GPW-2), red ginseng (GPR-1 and GPR-2, steamed ginseng at 100°C) and steamed ginseng (GPS-1 and GPS-2, steamed ginseng at 120°C) by combination of water extraction, ion-exchange and gel permeation chromatographies.
25263928	3	39	from	temperatures	713:724	arg1	fractions					739:747	these six fractions	729:747	these six fractions	729:747	Remarkably, GalA increased with the increase of processing temperatures in these six fractions, which might be caused by the transformation of esterified GalA into un-esterified form during heat processing.
25263928	1	40	theme	permeation	350:359	arg1	chromatographies					361:376	gel permeation chromatographies	346:376	gel permeation chromatographies	346:376	Six pectic polysaccharides were obtained from white ginseng (GPW-1 and GPW-2), red ginseng (GPR-1 and GPR-2, steamed ginseng at 100°C) and steamed ginseng (GPS-1 and GPS-2, steamed ginseng at 120°C) by combination of water extraction, ion-exchange and gel permeation chromatographies.
25263928	1	41	theme	pectic	98:103	arg1	polysaccharides					105:119	Six pectic polysaccharides	94:119	Six pectic polysaccharides	94:119	Six pectic polysaccharides were obtained from white ginseng (GPW-1 and GPW-2), red ginseng (GPR-1 and GPR-2, steamed ginseng at 100°C) and steamed ginseng (GPS-1 and GPS-2, steamed ginseng at 120°C) by combination of water extraction, ion-exchange and gel permeation chromatographies.
25263928	3	42	theme	temperatures	713:724	arg1	increase					690:697	the increase	686:697	the increase	686:697	Remarkably, GalA increased with the increase of processing temperatures in these six fractions, which might be caused by the transformation of esterified GalA into un-esterified form during heat processing.
25263928	1	43	theme	chromatographies	361:376	arg1	combination					296:306	combination	296:306	combination of water extraction, ion-exchange and gel permeation chromatographies	296:376	Six pectic polysaccharides were obtained from white ginseng (GPW-1 and GPW-2), red ginseng (GPR-1 and GPR-2, steamed ginseng at 100°C) and steamed ginseng (GPS-1 and GPS-2, steamed ginseng at 120°C) by combination of water extraction, ion-exchange and gel permeation chromatographies.
25263928	2	44	theme	methyl-esterification	631:651	arg1	degrees					620:626	different degrees	610:626	different degrees of methyl-esterification	610:651	Based on the data from monosaccharide composition and (13)C NMR analysis, GPW-1, GPR-1 and GPS-1 were identified as type-I rhamnogalacturonan (RG-I)-rich pectins, GPW-2, GPR-2 and GPS-2 were homogalacturonan (HG)-rich pectins with different degrees of methyl-esterification.
25263928	1	45	theme	white	140:144	arg1	ginseng					146:152	white ginseng	140:152	white ginseng (GPW-1 and GPW-2)	140:170	Six pectic polysaccharides were obtained from white ginseng (GPW-1 and GPW-2), red ginseng (GPR-1 and GPR-2, steamed ginseng at 100°C) and steamed ginseng (GPS-1 and GPS-2, steamed ginseng at 120°C) by combination of water extraction, ion-exchange and gel permeation chromatographies.
25263928	4	46	dep	In	861:862	arg1	vivo					864:867	vivo	864:867	vivo	864:867	In vivo animal experiments showed that GPs exhibited significant antiohyperglycemic and antioxidant activities in alloxan-induced diabetic mice, and the effects increased with the processing temperature, with the most potent activity in GPS.
25263928	4	47	theme	potent	1079:1084	arg1	activity					1086:1093	the most potent activity	1070:1093	the most potent activity in GPS	1070:1100	In vivo animal experiments showed that GPs exhibited significant antiohyperglycemic and antioxidant activities in alloxan-induced diabetic mice, and the effects increased with the processing temperature, with the most potent activity in GPS.
25263928	2	48	theme	C	437:437	arg1	analysis					443:450	(13)C NMR analysis	433:450	(13)C NMR analysis	433:450	Based on the data from monosaccharide composition and (13)C NMR analysis, GPW-1, GPR-1 and GPS-1 were identified as type-I rhamnogalacturonan (RG-I)-rich pectins, GPW-2, GPR-2 and GPS-2 were homogalacturonan (HG)-rich pectins with different degrees of methyl-esterification.
25263928	1	49	dep	ginseng	177:183	arg1	GPR-2					196:200	GPR-2	196:200	GPR-2	196:200	Six pectic polysaccharides were obtained from white ginseng (GPW-1 and GPW-2), red ginseng (GPR-1 and GPR-2, steamed ginseng at 100°C) and steamed ginseng (GPS-1 and GPS-2, steamed ginseng at 120°C) by combination of water extraction, ion-exchange and gel permeation chromatographies.
25263928	1	49	dep	ginseng	177:183	arg1	ginseng					211:217	steamed ginseng	203:217	steamed ginseng at 100°C	203:226	Six pectic polysaccharides were obtained from white ginseng (GPW-1 and GPW-2), red ginseng (GPR-1 and GPR-2, steamed ginseng at 100°C) and steamed ginseng (GPS-1 and GPS-2, steamed ginseng at 120°C) by combination of water extraction, ion-exchange and gel permeation chromatographies.
25263928	1	49	dep	ginseng	177:183	arg1	GPR-1					186:190	GPR-1	186:190	GPR-1	186:190	Six pectic polysaccharides were obtained from white ginseng (GPW-1 and GPW-2), red ginseng (GPR-1 and GPR-2, steamed ginseng at 100°C) and steamed ginseng (GPS-1 and GPS-2, steamed ginseng at 120°C) by combination of water extraction, ion-exchange and gel permeation chromatographies.
25263928	2	50	from	composition	417:427	arg1	data					392:395	the data	388:395	the data from monosaccharide composition and (13)C NMR analysis	388:450	Based on the data from monosaccharide composition and (13)C NMR analysis, GPW-1, GPR-1 and GPS-1 were identified as type-I rhamnogalacturonan (RG-I)-rich pectins, GPW-2, GPR-2 and GPS-2 were homogalacturonan (HG)-rich pectins with different degrees of methyl-esterification.
25263928	1	51	theme	steamed	203:209	arg1	ginseng					211:217	steamed ginseng	203:217	steamed ginseng at 100°C	203:226	Six pectic polysaccharides were obtained from white ginseng (GPW-1 and GPW-2), red ginseng (GPR-1 and GPR-2, steamed ginseng at 100°C) and steamed ginseng (GPS-1 and GPS-2, steamed ginseng at 120°C) by combination of water extraction, ion-exchange and gel permeation chromatographies.
25263928	1	51	theme	steamed	203:209	arg1	GPR-1					186:190	GPR-1	186:190	GPR-1	186:190	Six pectic polysaccharides were obtained from white ginseng (GPW-1 and GPW-2), red ginseng (GPR-1 and GPR-2, steamed ginseng at 100°C) and steamed ginseng (GPS-1 and GPS-2, steamed ginseng at 120°C) by combination of water extraction, ion-exchange and gel permeation chromatographies.
25263928	2	52	theme	monosaccharide	402:415	arg1	composition					417:427	monosaccharide composition	402:427	monosaccharide composition	402:427	Based on the data from monosaccharide composition and (13)C NMR analysis, GPW-1, GPR-1 and GPS-1 were identified as type-I rhamnogalacturonan (RG-I)-rich pectins, GPW-2, GPR-2 and GPS-2 were homogalacturonan (HG)-rich pectins with different degrees of methyl-esterification.
25263928	2	53	with	pectins	597:603	arg1	degrees					620:626	different degrees	610:626	different degrees of methyl-esterification	610:651	Based on the data from monosaccharide composition and (13)C NMR analysis, GPW-1, GPR-1 and GPS-1 were identified as type-I rhamnogalacturonan (RG-I)-rich pectins, GPW-2, GPR-2 and GPS-2 were homogalacturonan (HG)-rich pectins with different degrees of methyl-esterification.
25263928	3	54	theme	processing	702:711	arg1	temperatures					713:724	processing temperatures	702:724	processing temperatures in these six fractions	702:747	Remarkably, GalA increased with the increase of processing temperatures in these six fractions, which might be caused by the transformation of esterified GalA into un-esterified form during heat processing.
25735403	6	0	theme	expression	1125:1134	arg1	analysis					1136:1143	Gene expression analysis	1120:1143	Gene expression analysis of these cells	1120:1158	Gene expression analysis of these cells indicated an overexpression of genes specific for the biosynthesis of monolignol units of lignin.
25735403	2	1	theme	cell	329:332	arg1	polysaccharides					346:360	cell wall matrix polysaccharides	329:360	cell wall matrix polysaccharides	329:360	Cellulose deficiency was accompanied by marked changes in cell wall matrix polysaccharides and phenolics as revealed by Fourier transform infrared (FTIR) spectroscopy.
25735403	3	2	theme	cellulose-deficient	491:509	arg1	walls					516:520	the cellulose-deficient cell walls	487:520	the cellulose-deficient cell walls	487:520	Cell wall compositional analysis indicated that the cellulose-deficient cell walls showed an enhancement of highly branched and cross-linked arabinoxylans, as well as an increased content in ferulic acid, diferulates and p-coumaric acid, and the presence of a polymer that stained positive for phloroglucinol.
25735403	4	3	theme	Klason-type	835:845	arg1	lignin					847:852	Klason-type lignin	835:852	Klason-type lignin	835:852	In accordance with this, cellulose-deficient cell walls showed a fivefold increase in Klason-type lignin.
25735403	2	4	from	changes	318:324	arg1	phenolics					366:374	phenolics	366:374	phenolics	366:374	Cellulose deficiency was accompanied by marked changes in cell wall matrix polysaccharides and phenolics as revealed by Fourier transform infrared (FTIR) spectroscopy.
25735403	2	4	from	changes	318:324	arg1	polysaccharides					346:360	cell wall matrix polysaccharides	329:360	cell wall matrix polysaccharides	329:360	Cellulose deficiency was accompanied by marked changes in cell wall matrix polysaccharides and phenolics as revealed by Fourier transform infrared (FTIR) spectroscopy.
25735403	6	5	theme	Gene	1120:1123	arg1	analysis					1136:1143	Gene expression analysis	1120:1143	Gene expression analysis of these cells	1120:1158	Gene expression analysis of these cells indicated an overexpression of genes specific for the biosynthesis of monolignol units of lignin.
25735403	6	6	theme	specific	1197:1204	arg1	genes					1191:1195	genes	1191:1195	genes specific for the biosynthesis of monolignol units of lignin	1191:1255	Gene expression analysis of these cells indicated an overexpression of genes specific for the biosynthesis of monolignol units of lignin.
25735403	3	7	theme	arabinoxylans	580:592	arg1	presence					685:692	the presence	681:692	the presence of a polymer	681:705	Cell wall compositional analysis indicated that the cellulose-deficient cell walls showed an enhancement of highly branched and cross-linked arabinoxylans, as well as an increased content in ferulic acid, diferulates and p-coumaric acid, and the presence of a polymer that stained positive for phloroglucinol.
25735403	3	7	theme	arabinoxylans	580:592	arg1	content					619:625	an increased content	606:625	an increased content in ferulic acid, diferulates and p-coumaric acid	606:674	Cell wall compositional analysis indicated that the cellulose-deficient cell walls showed an enhancement of highly branched and cross-linked arabinoxylans, as well as an increased content in ferulic acid, diferulates and p-coumaric acid, and the presence of a polymer that stained positive for phloroglucinol.
25735403	3	7	theme	arabinoxylans	580:592	arg1	enhancement					532:542	an enhancement	529:542	an enhancement of highly branched and cross-linked arabinoxylans	529:592	Cell wall compositional analysis indicated that the cellulose-deficient cell walls showed an enhancement of highly branched and cross-linked arabinoxylans, as well as an increased content in ferulic acid, diferulates and p-coumaric acid, and the presence of a polymer that stained positive for phloroglucinol.
25735403	5	8	theme	cellulose-deficient	888:906	arg1	walls					913:917	cellulose-deficient cell walls	888:917	cellulose-deficient cell walls	888:917	Thioacidolysis/GC-MS analysis of cellulose-deficient cell walls indicated the presence of a lignin-like polymer with a Syringyl/Guaiacyl ratio of 1.45, which differed from the sensu stricto stress-related lignin that arose in response to short-term DCB-treatments.
25735403	7	9	theme	wall	1434:1437	arg1	integrity					1439:1447	cell wall integrity disruptions	1429:1459	cell wall integrity disruptions	1429:1459	A study of stress signaling pathways revealed an overexpression of some of the jasmonate signaling pathway genes, which might trigger ectopic lignification in response to cell wall integrity disruptions.
25735403	2	10	theme	Cellulose	271:279	arg1	deficiency					281:290	Cellulose deficiency	271:290	Cellulose deficiency	271:290	Cellulose deficiency was accompanied by marked changes in cell wall matrix polysaccharides and phenolics as revealed by Fourier transform infrared (FTIR) spectroscopy.
25735403	8	11	from	summary	1465:1471	arg1	plasticity					1489:1498	the structural plasticity	1474:1498	the structural plasticity of primary cell walls	1474:1520	In summary, the structural plasticity of primary cell walls is proven, since a lignification process is possible in response to cellulose impoverishment.
25735403	8	11	from	summary	1465:1471	arg1	proven					1525:1530	proven	1525:1530	proven	1525:1530	In summary, the structural plasticity of primary cell walls is proven, since a lignification process is possible in response to cellulose impoverishment.
25735403	8	12	theme	structural	1478:1487	arg1	plasticity					1489:1498	the structural plasticity	1474:1498	the structural plasticity of primary cell walls	1474:1520	In summary, the structural plasticity of primary cell walls is proven, since a lignification process is possible in response to cellulose impoverishment.
25735403	8	12	theme	structural	1478:1487	arg1	proven					1525:1530	proven	1525:1530	proven	1525:1530	In summary, the structural plasticity of primary cell walls is proven, since a lignification process is possible in response to cellulose impoverishment.
25735403	4	13	from	increase	823:830	arg1	lignin					847:852	Klason-type lignin	835:852	Klason-type lignin	835:852	In accordance with this, cellulose-deficient cell walls showed a fivefold increase in Klason-type lignin.
25735403	1	14	theme	stepwise	192:199	arg1	habituation					201:211	stepwise habituation	192:211	stepwise habituation to dichlobenil (DCB), a cellulose biosynthesis inhibitor	192:268	Maize (Zea mays L.) suspension-cultured cells with up to 70% less cellulose were obtained by stepwise habituation to dichlobenil (DCB), a cellulose biosynthesis inhibitor.
25735403	8	15	dep	impoverishment	1600:1613	arg1	response					1578:1585	response	1578:1585	response	1578:1585	In summary, the structural plasticity of primary cell walls is proven, since a lignification process is possible in response to cellulose impoverishment.
25735403	8	16	theme	cell	1511:1514	arg1	walls					1516:1520	primary cell walls	1503:1520	primary cell walls	1503:1520	In summary, the structural plasticity of primary cell walls is proven, since a lignification process is possible in response to cellulose impoverishment.
25735403	6	17	theme	lignin	1250:1255	arg1	units					1241:1245	monolignol units	1230:1245	monolignol units of lignin	1230:1255	Gene expression analysis of these cells indicated an overexpression of genes specific for the biosynthesis of monolignol units of lignin.
25735403	7	18	theme	ectopic	1392:1398	arg1	lignification					1400:1412	ectopic lignification	1392:1412	ectopic lignification	1392:1412	A study of stress signaling pathways revealed an overexpression of some of the jasmonate signaling pathway genes, which might trigger ectopic lignification in response to cell wall integrity disruptions.
25735403	5	19	theme	lignin-like	947:957	arg1	polymer					959:965	a lignin-like polymer	945:965	a lignin-like polymer with a Syringyl/Guaiacyl ratio of 1.45, which differed from the sensu stricto stress-related lignin that arose in response to short-term DCB-treatments	945:1117	Thioacidolysis/GC-MS analysis of cellulose-deficient cell walls indicated the presence of a lignin-like polymer with a Syringyl/Guaiacyl ratio of 1.45, which differed from the sensu stricto stress-related lignin that arose in response to short-term DCB-treatments.
25735403	6	20	theme	units	1241:1245	arg1	biosynthesis					1214:1225	the biosynthesis	1210:1225	the biosynthesis of monolignol units of lignin	1210:1255	Gene expression analysis of these cells indicated an overexpression of genes specific for the biosynthesis of monolignol units of lignin.
25735403	3	21	theme	wall	444:447	arg1	analysis					463:470	Cell wall compositional analysis	439:470	Cell wall compositional analysis	439:470	Cell wall compositional analysis indicated that the cellulose-deficient cell walls showed an enhancement of highly branched and cross-linked arabinoxylans, as well as an increased content in ferulic acid, diferulates and p-coumaric acid, and the presence of a polymer that stained positive for phloroglucinol.
25735403	0	22	theme	maize	67:71	arg1	cultures					89:96	maize cell suspension cultures	67:96	maize cell suspension cultures	67:96	Ectopic lignification in primary cellulose-deficient cell walls of maize cell suspension cultures.
25735403	3	23	theme	increased	609:617	arg1	content					619:625	an increased content	606:625	an increased content in ferulic acid, diferulates and p-coumaric acid	606:674	Cell wall compositional analysis indicated that the cellulose-deficient cell walls showed an enhancement of highly branched and cross-linked arabinoxylans, as well as an increased content in ferulic acid, diferulates and p-coumaric acid, and the presence of a polymer that stained positive for phloroglucinol.
25735403	5	24	theme	walls	913:917	arg1	analysis					876:883	Thioacidolysis/GC-MS analysis	855:883	Thioacidolysis/GC-MS analysis of cellulose-deficient cell walls	855:917	Thioacidolysis/GC-MS analysis of cellulose-deficient cell walls indicated the presence of a lignin-like polymer with a Syringyl/Guaiacyl ratio of 1.45, which differed from the sensu stricto stress-related lignin that arose in response to short-term DCB-treatments.
25735403	3	25	link	cross-linked	567:578	arg1	arabinoxylans					580:592	highly branched and cross-linked arabinoxylans	547:592	highly branched and cross-linked arabinoxylans	547:592	Cell wall compositional analysis indicated that the cellulose-deficient cell walls showed an enhancement of highly branched and cross-linked arabinoxylans, as well as an increased content in ferulic acid, diferulates and p-coumaric acid, and the presence of a polymer that stained positive for phloroglucinol.
25735403	3	26	from	enhancement	532:542	arg1	diferulates					644:654	diferulates	644:654	diferulates	644:654	Cell wall compositional analysis indicated that the cellulose-deficient cell walls showed an enhancement of highly branched and cross-linked arabinoxylans, as well as an increased content in ferulic acid, diferulates and p-coumaric acid, and the presence of a polymer that stained positive for phloroglucinol.
25735403	3	26	from	enhancement	532:542	arg1	acid					671:674	p-coumaric acid	660:674	p-coumaric acid	660:674	Cell wall compositional analysis indicated that the cellulose-deficient cell walls showed an enhancement of highly branched and cross-linked arabinoxylans, as well as an increased content in ferulic acid, diferulates and p-coumaric acid, and the presence of a polymer that stained positive for phloroglucinol.
25735403	3	26	from	enhancement	532:542	arg1	acid					638:641	ferulic acid	630:641	ferulic acid	630:641	Cell wall compositional analysis indicated that the cellulose-deficient cell walls showed an enhancement of highly branched and cross-linked arabinoxylans, as well as an increased content in ferulic acid, diferulates and p-coumaric acid, and the presence of a polymer that stained positive for phloroglucinol.
25735403	0	27	theme	suspension	78:87	arg1	cultures					89:96	maize cell suspension cultures	67:96	maize cell suspension cultures	67:96	Ectopic lignification in primary cellulose-deficient cell walls of maize cell suspension cultures.
25735403	1	28	theme	Zea	106:108	arg1	Maize					99:103	Maize	99:103	Maize (Zea mays L.) suspension-cultured cells with up to 70% less cellulose	99:173	Maize (Zea mays L.) suspension-cultured cells with up to 70% less cellulose were obtained by stepwise habituation to dichlobenil (DCB), a cellulose biosynthesis inhibitor.
25735403	1	28	theme	Zea	106:108	arg1	L.					115:116	Zea mays L.	106:116	Zea mays L.	106:116	Maize (Zea mays L.) suspension-cultured cells with up to 70% less cellulose were obtained by stepwise habituation to dichlobenil (DCB), a cellulose biosynthesis inhibitor.
25735403	1	29	theme	suspension-cultured	119:137	arg1	cells					139:143	Maize (Zea mays L.) suspension-cultured cells	99:143	Maize (Zea mays L.) suspension-cultured cells with up to 70% less cellulose	99:173	Maize (Zea mays L.) suspension-cultured cells with up to 70% less cellulose were obtained by stepwise habituation to dichlobenil (DCB), a cellulose biosynthesis inhibitor.
25735403	0	30	theme	Ectopic	0:6	arg1	lignification					8:20	Ectopic lignification	0:20	Ectopic lignification in primary cellulose-deficient cell walls of maize cell suspension cultures	0:96	Ectopic lignification in primary cellulose-deficient cell walls of maize cell suspension cultures.
25735403	1	31	theme	cellulose	237:245	arg1	inhibitor					260:268	a cellulose biosynthesis inhibitor	235:268	a cellulose biosynthesis inhibitor	235:268	Maize (Zea mays L.) suspension-cultured cells with up to 70% less cellulose were obtained by stepwise habituation to dichlobenil (DCB), a cellulose biosynthesis inhibitor.
25735403	1	31	theme	cellulose	237:245	arg1	dichlobenil					216:226	dichlobenil	216:226	dichlobenil (DCB)	216:232	Maize (Zea mays L.) suspension-cultured cells with up to 70% less cellulose were obtained by stepwise habituation to dichlobenil (DCB), a cellulose biosynthesis inhibitor.
25735403	1	32	theme	Maize	99:103	arg1	cells					139:143	Maize (Zea mays L.) suspension-cultured cells	99:143	Maize (Zea mays L.) suspension-cultured cells with up to 70% less cellulose	99:173	Maize (Zea mays L.) suspension-cultured cells with up to 70% less cellulose were obtained by stepwise habituation to dichlobenil (DCB), a cellulose biosynthesis inhibitor.
25735403	2	33	theme	infrared	409:416	arg1	spectroscopy					425:436	infrared (FTIR) spectroscopy	409:436	infrared (FTIR) spectroscopy	409:436	Cellulose deficiency was accompanied by marked changes in cell wall matrix polysaccharides and phenolics as revealed by Fourier transform infrared (FTIR) spectroscopy.
25735403	5	34	theme	Syringyl/Guaiacyl	974:990	arg1	ratio					992:996	a Syringyl/Guaiacyl ratio	972:996	a Syringyl/Guaiacyl ratio of 1.45, which differed from the sensu stricto stress-related lignin that arose in response to short-term DCB-treatments	972:1117	Thioacidolysis/GC-MS analysis of cellulose-deficient cell walls indicated the presence of a lignin-like polymer with a Syringyl/Guaiacyl ratio of 1.45, which differed from the sensu stricto stress-related lignin that arose in response to short-term DCB-treatments.
25735403	7	35	theme	signaling	1347:1355	arg1	genes					1365:1369	the jasmonate signaling pathway genes	1333:1369	the jasmonate signaling pathway genes	1333:1369	A study of stress signaling pathways revealed an overexpression of some of the jasmonate signaling pathway genes, which might trigger ectopic lignification in response to cell wall integrity disruptions.
25735403	8	36	theme	cellulose	1590:1598	arg1	impoverishment					1600:1613	cellulose impoverishment	1590:1613	cellulose impoverishment	1590:1613	In summary, the structural plasticity of primary cell walls is proven, since a lignification process is possible in response to cellulose impoverishment.
25735403	2	37	dep	Fourier	391:397	arg1	transform					399:407	transform	399:407	transform infrared (FTIR) spectroscopy	399:436	Cellulose deficiency was accompanied by marked changes in cell wall matrix polysaccharides and phenolics as revealed by Fourier transform infrared (FTIR) spectroscopy.
25735403	0	38	theme	cellulose-deficient	33:51	arg1	walls					58:62	primary cellulose-deficient cell walls	25:62	primary cellulose-deficient cell walls of maize cell suspension cultures	25:96	Ectopic lignification in primary cellulose-deficient cell walls of maize cell suspension cultures.
25735403	5	39	theme	stricto	1037:1043	arg1	lignin					1060:1065	the sensu stricto stress-related lignin	1027:1065	the sensu stricto stress-related lignin that arose in response to short-term DCB-treatments	1027:1117	Thioacidolysis/GC-MS analysis of cellulose-deficient cell walls indicated the presence of a lignin-like polymer with a Syringyl/Guaiacyl ratio of 1.45, which differed from the sensu stricto stress-related lignin that arose in response to short-term DCB-treatments.
25735403	2	40	dep	infrared	409:416	arg1	FTIR					419:422	FTIR	419:422	FTIR	419:422	Cellulose deficiency was accompanied by marked changes in cell wall matrix polysaccharides and phenolics as revealed by Fourier transform infrared (FTIR) spectroscopy.
25735403	7	41	theme	some	1325:1328	arg1	overexpression					1307:1320	an overexpression	1304:1320	an overexpression of some of the jasmonate signaling pathway genes, which might trigger ectopic lignification in response to cell wall integrity disruptions	1304:1459	A study of stress signaling pathways revealed an overexpression of some of the jasmonate signaling pathway genes, which might trigger ectopic lignification in response to cell wall integrity disruptions.
25735403	4	42	theme	cellulose-deficient	774:792	arg1	walls					799:803	cellulose-deficient cell walls	774:803	cellulose-deficient cell walls	774:803	In accordance with this, cellulose-deficient cell walls showed a fivefold increase in Klason-type lignin.
25735403	2	43	theme	marked	311:316	arg1	changes					318:324	marked changes	311:324	marked changes in cell wall matrix polysaccharides and phenolics	311:374	Cellulose deficiency was accompanied by marked changes in cell wall matrix polysaccharides and phenolics as revealed by Fourier transform infrared (FTIR) spectroscopy.
25735403	7	44	dep	integrity	1439:1447	arg1	disruptions					1449:1459	disruptions	1449:1459	cell wall integrity disruptions	1429:1459	A study of stress signaling pathways revealed an overexpression of some of the jasmonate signaling pathway genes, which might trigger ectopic lignification in response to cell wall integrity disruptions.
25735403	7	44	dep	integrity	1439:1447	arg1	response					1417:1424	response	1417:1424	response	1417:1424	A study of stress signaling pathways revealed an overexpression of some of the jasmonate signaling pathway genes, which might trigger ectopic lignification in response to cell wall integrity disruptions.
25735403	3	45	theme	p-coumaric	660:669	arg1	acid					671:674	p-coumaric acid	660:674	p-coumaric acid	660:674	Cell wall compositional analysis indicated that the cellulose-deficient cell walls showed an enhancement of highly branched and cross-linked arabinoxylans, as well as an increased content in ferulic acid, diferulates and p-coumaric acid, and the presence of a polymer that stained positive for phloroglucinol.
25735403	2	46	theme	wall	334:337	arg1	polysaccharides					346:360	cell wall matrix polysaccharides	329:360	cell wall matrix polysaccharides	329:360	Cellulose deficiency was accompanied by marked changes in cell wall matrix polysaccharides and phenolics as revealed by Fourier transform infrared (FTIR) spectroscopy.
25735403	7	47	theme	signaling	1276:1284	arg1	pathways					1286:1293	stress signaling pathways	1269:1293	stress signaling pathways	1269:1293	A study of stress signaling pathways revealed an overexpression of some of the jasmonate signaling pathway genes, which might trigger ectopic lignification in response to cell wall integrity disruptions.
25735403	1	48	dep	70	156:157	arg1	to					153:154	to	153:154	to	153:154	Maize (Zea mays L.) suspension-cultured cells with up to 70% less cellulose were obtained by stepwise habituation to dichlobenil (DCB), a cellulose biosynthesis inhibitor.
25735403	3	49	attach	presence	685:692	arg2	arabinoxylans					580:592	highly branched and cross-linked arabinoxylans	547:592	highly branched and cross-linked arabinoxylans	547:592	Cell wall compositional analysis indicated that the cellulose-deficient cell walls showed an enhancement of highly branched and cross-linked arabinoxylans, as well as an increased content in ferulic acid, diferulates and p-coumaric acid, and the presence of a polymer that stained positive for phloroglucinol.
25735403	3	49	attach	presence	685:692	arg2	polymer					699:705	a polymer	697:705	a polymer	697:705	Cell wall compositional analysis indicated that the cellulose-deficient cell walls showed an enhancement of highly branched and cross-linked arabinoxylans, as well as an increased content in ferulic acid, diferulates and p-coumaric acid, and the presence of a polymer that stained positive for phloroglucinol.
25735403	3	49	attach	presence	685:692	arg1	diferulates					644:654	diferulates	644:654	diferulates	644:654	Cell wall compositional analysis indicated that the cellulose-deficient cell walls showed an enhancement of highly branched and cross-linked arabinoxylans, as well as an increased content in ferulic acid, diferulates and p-coumaric acid, and the presence of a polymer that stained positive for phloroglucinol.
25735403	3	49	attach	presence	685:692	arg1	acid					671:674	p-coumaric acid	660:674	p-coumaric acid	660:674	Cell wall compositional analysis indicated that the cellulose-deficient cell walls showed an enhancement of highly branched and cross-linked arabinoxylans, as well as an increased content in ferulic acid, diferulates and p-coumaric acid, and the presence of a polymer that stained positive for phloroglucinol.
25735403	3	49	attach	presence	685:692	arg1	acid					638:641	ferulic acid	630:641	ferulic acid	630:641	Cell wall compositional analysis indicated that the cellulose-deficient cell walls showed an enhancement of highly branched and cross-linked arabinoxylans, as well as an increased content in ferulic acid, diferulates and p-coumaric acid, and the presence of a polymer that stained positive for phloroglucinol.
25735403	5	50	theme	Thioacidolysis/GC-MS	855:874	arg1	analysis					876:883	Thioacidolysis/GC-MS analysis	855:883	Thioacidolysis/GC-MS analysis of cellulose-deficient cell walls	855:917	Thioacidolysis/GC-MS analysis of cellulose-deficient cell walls indicated the presence of a lignin-like polymer with a Syringyl/Guaiacyl ratio of 1.45, which differed from the sensu stricto stress-related lignin that arose in response to short-term DCB-treatments.
25735403	3	51	theme	polymer	699:705	arg1	presence					685:692	the presence	681:692	the presence of a polymer	681:705	Cell wall compositional analysis indicated that the cellulose-deficient cell walls showed an enhancement of highly branched and cross-linked arabinoxylans, as well as an increased content in ferulic acid, diferulates and p-coumaric acid, and the presence of a polymer that stained positive for phloroglucinol.
25735403	3	51	theme	polymer	699:705	arg1	content					619:625	an increased content	606:625	an increased content in ferulic acid, diferulates and p-coumaric acid	606:674	Cell wall compositional analysis indicated that the cellulose-deficient cell walls showed an enhancement of highly branched and cross-linked arabinoxylans, as well as an increased content in ferulic acid, diferulates and p-coumaric acid, and the presence of a polymer that stained positive for phloroglucinol.
25735403	3	51	theme	polymer	699:705	arg1	enhancement					532:542	an enhancement	529:542	an enhancement of highly branched and cross-linked arabinoxylans	529:592	Cell wall compositional analysis indicated that the cellulose-deficient cell walls showed an enhancement of highly branched and cross-linked arabinoxylans, as well as an increased content in ferulic acid, diferulates and p-coumaric acid, and the presence of a polymer that stained positive for phloroglucinol.
25735403	3	52	theme	cross-linked	567:578	arg1	arabinoxylans					580:592	highly branched and cross-linked arabinoxylans	547:592	highly branched and cross-linked arabinoxylans	547:592	Cell wall compositional analysis indicated that the cellulose-deficient cell walls showed an enhancement of highly branched and cross-linked arabinoxylans, as well as an increased content in ferulic acid, diferulates and p-coumaric acid, and the presence of a polymer that stained positive for phloroglucinol.
25735403	1	53	theme	less	160:163	arg1	cellulose					165:173	up to 70% less cellulose	150:173	up to 70% less cellulose	150:173	Maize (Zea mays L.) suspension-cultured cells with up to 70% less cellulose were obtained by stepwise habituation to dichlobenil (DCB), a cellulose biosynthesis inhibitor.
25735403	8	54	theme	lignification	1541:1553	arg1	possible					1566:1573	possible	1566:1573	possible	1566:1573	In summary, the structural plasticity of primary cell walls is proven, since a lignification process is possible in response to cellulose impoverishment.
25735403	8	54	theme	lignification	1541:1553	arg1	process					1555:1561	a lignification process	1539:1561	a lignification process	1539:1561	In summary, the structural plasticity of primary cell walls is proven, since a lignification process is possible in response to cellulose impoverishment.
25735403	6	55	theme	genes	1191:1195	arg1	overexpression					1173:1186	an overexpression	1170:1186	an overexpression of genes specific for the biosynthesis of monolignol units of lignin	1170:1255	Gene expression analysis of these cells indicated an overexpression of genes specific for the biosynthesis of monolignol units of lignin.
25735403	5	56	with	polymer	959:965	arg1	ratio					992:996	a Syringyl/Guaiacyl ratio	972:996	a Syringyl/Guaiacyl ratio of 1.45, which differed from the sensu stricto stress-related lignin that arose in response to short-term DCB-treatments	972:1117	Thioacidolysis/GC-MS analysis of cellulose-deficient cell walls indicated the presence of a lignin-like polymer with a Syringyl/Guaiacyl ratio of 1.45, which differed from the sensu stricto stress-related lignin that arose in response to short-term DCB-treatments.
25735403	3	57	theme	branched	554:561	arg1	arabinoxylans					580:592	highly branched and cross-linked arabinoxylans	547:592	highly branched and cross-linked arabinoxylans	547:592	Cell wall compositional analysis indicated that the cellulose-deficient cell walls showed an enhancement of highly branched and cross-linked arabinoxylans, as well as an increased content in ferulic acid, diferulates and p-coumaric acid, and the presence of a polymer that stained positive for phloroglucinol.
25735403	5	58	theme	short-term	1093:1102	arg1	DCB-treatments					1104:1117	short-term DCB-treatments	1093:1117	short-term DCB-treatments	1093:1117	Thioacidolysis/GC-MS analysis of cellulose-deficient cell walls indicated the presence of a lignin-like polymer with a Syringyl/Guaiacyl ratio of 1.45, which differed from the sensu stricto stress-related lignin that arose in response to short-term DCB-treatments.
25735403	8	59	theme	primary	1503:1509	arg1	walls					1516:1520	primary cell walls	1503:1520	primary cell walls	1503:1520	In summary, the structural plasticity of primary cell walls is proven, since a lignification process is possible in response to cellulose impoverishment.
25735403	7	60	theme	cell	1429:1432	arg1	integrity					1439:1447	cell wall integrity disruptions	1429:1459	cell wall integrity disruptions	1429:1459	A study of stress signaling pathways revealed an overexpression of some of the jasmonate signaling pathway genes, which might trigger ectopic lignification in response to cell wall integrity disruptions.
25735403	3	61	from	presence	685:692	arg1	diferulates					644:654	diferulates	644:654	diferulates	644:654	Cell wall compositional analysis indicated that the cellulose-deficient cell walls showed an enhancement of highly branched and cross-linked arabinoxylans, as well as an increased content in ferulic acid, diferulates and p-coumaric acid, and the presence of a polymer that stained positive for phloroglucinol.
25735403	3	61	from	presence	685:692	arg1	acid					671:674	p-coumaric acid	660:674	p-coumaric acid	660:674	Cell wall compositional analysis indicated that the cellulose-deficient cell walls showed an enhancement of highly branched and cross-linked arabinoxylans, as well as an increased content in ferulic acid, diferulates and p-coumaric acid, and the presence of a polymer that stained positive for phloroglucinol.
25735403	3	61	from	presence	685:692	arg1	acid					638:641	ferulic acid	630:641	ferulic acid	630:641	Cell wall compositional analysis indicated that the cellulose-deficient cell walls showed an enhancement of highly branched and cross-linked arabinoxylans, as well as an increased content in ferulic acid, diferulates and p-coumaric acid, and the presence of a polymer that stained positive for phloroglucinol.
25735403	1	62	theme	mays	110:113	arg1	Maize					99:103	Maize	99:103	Maize (Zea mays L.) suspension-cultured cells with up to 70% less cellulose	99:173	Maize (Zea mays L.) suspension-cultured cells with up to 70% less cellulose were obtained by stepwise habituation to dichlobenil (DCB), a cellulose biosynthesis inhibitor.
25735403	1	62	theme	mays	110:113	arg1	L.					115:116	Zea mays L.	106:116	Zea mays L.	106:116	Maize (Zea mays L.) suspension-cultured cells with up to 70% less cellulose were obtained by stepwise habituation to dichlobenil (DCB), a cellulose biosynthesis inhibitor.
25735403	8	63	theme	walls	1516:1520	arg1	plasticity					1489:1498	the structural plasticity	1474:1498	the structural plasticity of primary cell walls	1474:1520	In summary, the structural plasticity of primary cell walls is proven, since a lignification process is possible in response to cellulose impoverishment.
25735403	8	63	theme	walls	1516:1520	arg1	proven					1525:1530	proven	1525:1530	proven	1525:1530	In summary, the structural plasticity of primary cell walls is proven, since a lignification process is possible in response to cellulose impoverishment.
25735403	0	64	theme	cell	73:76	arg1	cultures					89:96	maize cell suspension cultures	67:96	maize cell suspension cultures	67:96	Ectopic lignification in primary cellulose-deficient cell walls of maize cell suspension cultures.
25735403	5	65	theme	cell	908:911	arg1	walls					913:917	cellulose-deficient cell walls	888:917	cellulose-deficient cell walls	888:917	Thioacidolysis/GC-MS analysis of cellulose-deficient cell walls indicated the presence of a lignin-like polymer with a Syringyl/Guaiacyl ratio of 1.45, which differed from the sensu stricto stress-related lignin that arose in response to short-term DCB-treatments.
25735403	6	66	theme	monolignol	1230:1239	arg1	units					1241:1245	monolignol units	1230:1245	monolignol units of lignin	1230:1255	Gene expression analysis of these cells indicated an overexpression of genes specific for the biosynthesis of monolignol units of lignin.
25735403	0	67	theme	cultures	89:96	arg1	walls					58:62	primary cellulose-deficient cell walls	25:62	primary cellulose-deficient cell walls of maize cell suspension cultures	25:96	Ectopic lignification in primary cellulose-deficient cell walls of maize cell suspension cultures.
25735403	0	68	from	lignification	8:20	arg1	walls					58:62	primary cellulose-deficient cell walls	25:62	primary cellulose-deficient cell walls of maize cell suspension cultures	25:96	Ectopic lignification in primary cellulose-deficient cell walls of maize cell suspension cultures.
25735403	8	69	from	proven	1525:1530	arg1	summary					1465:1471	summary	1465:1471	summary	1465:1471	In summary, the structural plasticity of primary cell walls is proven, since a lignification process is possible in response to cellulose impoverishment.
25735403	1	70	with	cells	139:143	arg1	cellulose					165:173	up to 70% less cellulose	150:173	up to 70% less cellulose	150:173	Maize (Zea mays L.) suspension-cultured cells with up to 70% less cellulose were obtained by stepwise habituation to dichlobenil (DCB), a cellulose biosynthesis inhibitor.
25735403	5	71	theme	1.45	1001:1004	arg1	ratio					992:996	a Syringyl/Guaiacyl ratio	972:996	a Syringyl/Guaiacyl ratio of 1.45, which differed from the sensu stricto stress-related lignin that arose in response to short-term DCB-treatments	972:1117	Thioacidolysis/GC-MS analysis of cellulose-deficient cell walls indicated the presence of a lignin-like polymer with a Syringyl/Guaiacyl ratio of 1.45, which differed from the sensu stricto stress-related lignin that arose in response to short-term DCB-treatments.
25735403	3	72	from	content	619:625	arg1	diferulates					644:654	diferulates	644:654	diferulates	644:654	Cell wall compositional analysis indicated that the cellulose-deficient cell walls showed an enhancement of highly branched and cross-linked arabinoxylans, as well as an increased content in ferulic acid, diferulates and p-coumaric acid, and the presence of a polymer that stained positive for phloroglucinol.
25735403	3	72	from	content	619:625	arg1	acid					671:674	p-coumaric acid	660:674	p-coumaric acid	660:674	Cell wall compositional analysis indicated that the cellulose-deficient cell walls showed an enhancement of highly branched and cross-linked arabinoxylans, as well as an increased content in ferulic acid, diferulates and p-coumaric acid, and the presence of a polymer that stained positive for phloroglucinol.
25735403	3	72	from	content	619:625	arg1	acid					638:641	ferulic acid	630:641	ferulic acid	630:641	Cell wall compositional analysis indicated that the cellulose-deficient cell walls showed an enhancement of highly branched and cross-linked arabinoxylans, as well as an increased content in ferulic acid, diferulates and p-coumaric acid, and the presence of a polymer that stained positive for phloroglucinol.
25735403	5	73	theme	polymer	959:965	arg1	presence					933:940	the presence	929:940	the presence of a lignin-like polymer with a Syringyl/Guaiacyl ratio of 1.45, which differed from the sensu stricto stress-related lignin that arose in response to short-term DCB-treatments	929:1117	Thioacidolysis/GC-MS analysis of cellulose-deficient cell walls indicated the presence of a lignin-like polymer with a Syringyl/Guaiacyl ratio of 1.45, which differed from the sensu stricto stress-related lignin that arose in response to short-term DCB-treatments.
25735403	7	74	theme	pathway	1357:1363	arg1	genes					1365:1369	the jasmonate signaling pathway genes	1333:1369	the jasmonate signaling pathway genes	1333:1369	A study of stress signaling pathways revealed an overexpression of some of the jasmonate signaling pathway genes, which might trigger ectopic lignification in response to cell wall integrity disruptions.
25735403	0	75	theme	primary	25:31	arg1	walls					58:62	primary cellulose-deficient cell walls	25:62	primary cellulose-deficient cell walls of maize cell suspension cultures	25:96	Ectopic lignification in primary cellulose-deficient cell walls of maize cell suspension cultures.
25735403	1	76	theme	biosynthesis	247:258	arg1	inhibitor					260:268	a cellulose biosynthesis inhibitor	235:268	a cellulose biosynthesis inhibitor	235:268	Maize (Zea mays L.) suspension-cultured cells with up to 70% less cellulose were obtained by stepwise habituation to dichlobenil (DCB), a cellulose biosynthesis inhibitor.
25735403	1	76	theme	biosynthesis	247:258	arg1	dichlobenil					216:226	dichlobenil	216:226	dichlobenil (DCB)	216:232	Maize (Zea mays L.) suspension-cultured cells with up to 70% less cellulose were obtained by stepwise habituation to dichlobenil (DCB), a cellulose biosynthesis inhibitor.
25735403	7	77	theme	jasmonate	1337:1345	arg1	genes					1365:1369	the jasmonate signaling pathway genes	1333:1369	the jasmonate signaling pathway genes	1333:1369	A study of stress signaling pathways revealed an overexpression of some of the jasmonate signaling pathway genes, which might trigger ectopic lignification in response to cell wall integrity disruptions.
25735403	3	78	theme	compositional	449:461	arg1	analysis					463:470	Cell wall compositional analysis	439:470	Cell wall compositional analysis	439:470	Cell wall compositional analysis indicated that the cellulose-deficient cell walls showed an enhancement of highly branched and cross-linked arabinoxylans, as well as an increased content in ferulic acid, diferulates and p-coumaric acid, and the presence of a polymer that stained positive for phloroglucinol.
25735403	0	79	theme	cell	53:56	arg1	walls					58:62	primary cellulose-deficient cell walls	25:62	primary cellulose-deficient cell walls of maize cell suspension cultures	25:96	Ectopic lignification in primary cellulose-deficient cell walls of maize cell suspension cultures.
25735403	5	80	theme	sensu	1031:1035	arg1	stricto					1037:1043	sensu stricto	1031:1043	the sensu stricto stress-related lignin that arose in response to short-term DCB-treatments	1027:1117	Thioacidolysis/GC-MS analysis of cellulose-deficient cell walls indicated the presence of a lignin-like polymer with a Syringyl/Guaiacyl ratio of 1.45, which differed from the sensu stricto stress-related lignin that arose in response to short-term DCB-treatments.
25735403	3	81	theme	ferulic	630:636	arg1	acid					638:641	ferulic acid	630:641	ferulic acid	630:641	Cell wall compositional analysis indicated that the cellulose-deficient cell walls showed an enhancement of highly branched and cross-linked arabinoxylans, as well as an increased content in ferulic acid, diferulates and p-coumaric acid, and the presence of a polymer that stained positive for phloroglucinol.
25735403	3	82	theme	Cell	439:442	arg1	analysis					463:470	Cell wall compositional analysis	439:470	Cell wall compositional analysis	439:470	Cell wall compositional analysis indicated that the cellulose-deficient cell walls showed an enhancement of highly branched and cross-linked arabinoxylans, as well as an increased content in ferulic acid, diferulates and p-coumaric acid, and the presence of a polymer that stained positive for phloroglucinol.
25735403	5	83	theme	stress-related	1045:1058	arg1	lignin					1060:1065	the sensu stricto stress-related lignin	1027:1065	the sensu stricto stress-related lignin that arose in response to short-term DCB-treatments	1027:1117	Thioacidolysis/GC-MS analysis of cellulose-deficient cell walls indicated the presence of a lignin-like polymer with a Syringyl/Guaiacyl ratio of 1.45, which differed from the sensu stricto stress-related lignin that arose in response to short-term DCB-treatments.
25735403	4	84	theme	fivefold	814:821	arg1	increase					823:830	a fivefold increase	812:830	a fivefold increase in Klason-type lignin	812:852	In accordance with this, cellulose-deficient cell walls showed a fivefold increase in Klason-type lignin.
25735403	3	85	theme	cell	511:514	arg1	walls					516:520	the cellulose-deficient cell walls	487:520	the cellulose-deficient cell walls	487:520	Cell wall compositional analysis indicated that the cellulose-deficient cell walls showed an enhancement of highly branched and cross-linked arabinoxylans, as well as an increased content in ferulic acid, diferulates and p-coumaric acid, and the presence of a polymer that stained positive for phloroglucinol.
25735403	6	86	theme	cells	1154:1158	arg1	analysis					1136:1143	Gene expression analysis	1120:1143	Gene expression analysis of these cells	1120:1158	Gene expression analysis of these cells indicated an overexpression of genes specific for the biosynthesis of monolignol units of lignin.
25735403	5	87	dep	DCB-treatments	1104:1117	arg1	response					1081:1088	response	1081:1088	response	1081:1088	Thioacidolysis/GC-MS analysis of cellulose-deficient cell walls indicated the presence of a lignin-like polymer with a Syringyl/Guaiacyl ratio of 1.45, which differed from the sensu stricto stress-related lignin that arose in response to short-term DCB-treatments.
25735403	7	88	theme	pathways	1286:1293	arg1	study					1260:1264	A study	1258:1264	A study of stress signaling pathways	1258:1293	A study of stress signaling pathways revealed an overexpression of some of the jasmonate signaling pathway genes, which might trigger ectopic lignification in response to cell wall integrity disruptions.
25735403	4	89	theme	cell	794:797	arg1	walls					799:803	cellulose-deficient cell walls	774:803	cellulose-deficient cell walls	774:803	In accordance with this, cellulose-deficient cell walls showed a fivefold increase in Klason-type lignin.
25735403	2	90	theme	matrix	339:344	arg1	polysaccharides					346:360	cell wall matrix polysaccharides	329:360	cell wall matrix polysaccharides	329:360	Cellulose deficiency was accompanied by marked changes in cell wall matrix polysaccharides and phenolics as revealed by Fourier transform infrared (FTIR) spectroscopy.
25735403	7	91	theme	stress	1269:1274	arg1	pathways					1286:1293	stress signaling pathways	1269:1293	stress signaling pathways	1269:1293	A study of stress signaling pathways revealed an overexpression of some of the jasmonate signaling pathway genes, which might trigger ectopic lignification in response to cell wall integrity disruptions.
28715109	5	0	theme	pH	699:700	arg1	value					702:706	pH value	699:706	pH value	699:706	The effects of sample flow rate, sample volume, and pH value were optimized.
28715109	1	1	theme	multiwalled	177:187	arg1	composite					221:229	multiwalled carbon nanotubes-β-cyclodextrin composite	177:229	multiwalled carbon nanotubes-β-cyclodextrin composite	177:229	A polymeric column that contains multiwalled carbon nanotubes-β-cyclodextrin composite was developed.
28715109	0	2	theme	selective	94:102	arg1	extraction					104:113	the selective extraction	90:113	the selective extraction of psoralen and isopsoralen	90:141	Polymeric monolith column composited with multiwalled carbon nanotubes-β-cyclodextrin for the selective extraction of psoralen and isopsoralen.
28715109	4	3	theme	liquid	580:585	arg1	chromatography					587:600	high-performance liquid chromatography	563:600	high-performance liquid chromatography with C18 separation column and UV detection	563:644	Following microextraction, the coumarins were quantified by high-performance liquid chromatography with C18 separation column and UV detection.
28715109	5	4	theme	value	702:706	arg1	effects					651:657	The effects	647:657	The effects of sample flow rate, sample volume, and pH value	647:706	The effects of sample flow rate, sample volume, and pH value were optimized.
28715109	5	5	theme	sample	662:667	arg1	rate					674:677	sample flow rate	662:677	sample flow rate	662:677	The effects of sample flow rate, sample volume, and pH value were optimized.
28715109	1	6	theme	carbon	189:194	arg1	composite					221:229	multiwalled carbon nanotubes-β-cyclodextrin composite	177:229	multiwalled carbon nanotubes-β-cyclodextrin composite	177:229	A polymeric column that contains multiwalled carbon nanotubes-β-cyclodextrin composite was developed.
28715109	2	7	from	10 cm	362:366	arg1	length					371:376	length	371:376	length	371:376	The composite was wrapped into the poly(butyl methacrylate-ethylene dimethacrylate) monolith column (0.76 mm id and 10 cm in length).
28715109	0	8	theme	psoralen	118:125	arg1	extraction					104:113	the selective extraction	90:113	the selective extraction of psoralen and isopsoralen	90:141	Polymeric monolith column composited with multiwalled carbon nanotubes-β-cyclodextrin for the selective extraction of psoralen and isopsoralen.
28715109	4	9	theme	high-performance	563:578	arg1	chromatography					587:600	high-performance liquid chromatography	563:600	high-performance liquid chromatography with C18 separation column and UV detection	563:644	Following microextraction, the coumarins were quantified by high-performance liquid chromatography with C18 separation column and UV detection.
28715109	8	10	theme	hydrophobicity	1072:1085	arg1	cavities					1037:1044	The empty hydrophobic cavities	1015:1044	The empty hydrophobic cavities of β-cyclodextrin and the hydrophobicity of multiwalled carbon nanotubes	1015:1117	The empty hydrophobic cavities of β-cyclodextrin and the hydrophobicity of multiwalled carbon nanotubes provided specific extraction capability for psoralen and isopsoralen.
28715109	5	11	theme	flow	669:672	arg1	rate					674:677	sample flow rate	662:677	sample flow rate	662:677	The effects of sample flow rate, sample volume, and pH value were optimized.
28715109	4	12	with	chromatography	587:600	arg1	column					622:627	C18 separation column	607:627	C18 separation column	607:627	Following microextraction, the coumarins were quantified by high-performance liquid chromatography with C18 separation column and UV detection.
28715109	4	12	with	chromatography	587:600	arg1	detection					636:644	UV detection	633:644	UV detection	633:644	Following microextraction, the coumarins were quantified by high-performance liquid chromatography with C18 separation column and UV detection.
28715109	5	13	theme	rate	674:677	arg1	effects					651:657	The effects	647:657	The effects of sample flow rate, sample volume, and pH value	647:706	The effects of sample flow rate, sample volume, and pH value were optimized.
28715109	6	14	theme	detection	756:764	arg1	limits					746:751	low limits	742:751	low limits of detection (20 pg/mL, S/N = 3) for both psoralen and isopsoralen	742:818	The method showed low limits of detection (20 pg/mL, S/N = 3) for both psoralen and isopsoralen.
28715109	7	15	theme	psoralen	889:896	arg1	determination					872:884	the determination	868:884	the determination of psoralen	868:896	Finally the method was successfully applied to the determination of psoralen and isopsoralen in spiked herb extracts and rat plasma where it gave recoveries that ranged between 93.2 and 102.1%.
28715109	0	16	theme	isopsoralen	131:141	arg1	extraction					104:113	the selective extraction	90:113	the selective extraction of psoralen and isopsoralen	90:141	Polymeric monolith column composited with multiwalled carbon nanotubes-β-cyclodextrin for the selective extraction of psoralen and isopsoralen.
28715109	5	17	theme	sample	680:685	arg1	volume					687:692	sample volume	680:692	sample volume	680:692	The effects of sample flow rate, sample volume, and pH value were optimized.
28715109	2	18	theme	monolith	330:337	arg1	column					339:344	the poly(butyl methacrylate-ethylene dimethacrylate) monolith column	277:344	the poly(butyl methacrylate-ethylene dimethacrylate) monolith column (0.76 mm id and 10 cm in length)	277:377	The composite was wrapped into the poly(butyl methacrylate-ethylene dimethacrylate) monolith column (0.76 mm id and 10 cm in length).
28715109	1	19	theme	nanotubes-β-cyclodextrin	196:219	arg1	composite					221:229	multiwalled carbon nanotubes-β-cyclodextrin composite	177:229	multiwalled carbon nanotubes-β-cyclodextrin composite	177:229	A polymeric column that contains multiwalled carbon nanotubes-β-cyclodextrin composite was developed.
28715109	1	20	contain	contains	168:175	arg1	column					156:161	A polymeric column	144:161	A polymeric column that contains multiwalled carbon nanotubes-β-cyclodextrin composite	144:229	A polymeric column that contains multiwalled carbon nanotubes-β-cyclodextrin composite was developed.
28715109	1	20	contain	contains	168:175	arg2	composite					221:229	multiwalled carbon nanotubes-β-cyclodextrin composite	177:229	multiwalled carbon nanotubes-β-cyclodextrin composite	177:229	A polymeric column that contains multiwalled carbon nanotubes-β-cyclodextrin composite was developed.
28715109	5	21	theme	volume	687:692	arg1	effects					651:657	The effects	647:657	The effects of sample flow rate, sample volume, and pH value	647:706	The effects of sample flow rate, sample volume, and pH value were optimized.
28715109	8	22	theme	hydrophobic	1025:1035	arg1	cavities					1037:1044	The empty hydrophobic cavities	1015:1044	The empty hydrophobic cavities of β-cyclodextrin and the hydrophobicity of multiwalled carbon nanotubes	1015:1117	The empty hydrophobic cavities of β-cyclodextrin and the hydrophobicity of multiwalled carbon nanotubes provided specific extraction capability for psoralen and isopsoralen.
28715109	3	23	theme	online	416:421	arg1	microextraction					435:449	the online solid-phase microextraction	412:449	the online solid-phase microextraction of psoralen and isopsoralen from Fructus Psoraleae	412:500	The column was then applied for the online solid-phase microextraction of psoralen and isopsoralen from Fructus Psoraleae.
28715109	0	24	theme	monolith	10:17	arg1	column					19:24	Polymeric monolith column	0:24	Polymeric monolith column	0:24	Polymeric monolith column composited with multiwalled carbon nanotubes-β-cyclodextrin for the selective extraction of psoralen and isopsoralen.
28715109	2	25	dep	column	339:344	arg1	id					355:356	0.76 mm id	347:356	0.76 mm id	347:356	The composite was wrapped into the poly(butyl methacrylate-ethylene dimethacrylate) monolith column (0.76 mm id and 10 cm in length).
28715109	2	25	dep	column	339:344	arg1	10 cm					362:366	10 cm	362:366	10 cm in length	362:376	The composite was wrapped into the poly(butyl methacrylate-ethylene dimethacrylate) monolith column (0.76 mm id and 10 cm in length).
28715109	8	26	theme	empty	1019:1023	arg1	cavities					1037:1044	The empty hydrophobic cavities	1015:1044	The empty hydrophobic cavities of β-cyclodextrin and the hydrophobicity of multiwalled carbon nanotubes	1015:1117	The empty hydrophobic cavities of β-cyclodextrin and the hydrophobicity of multiwalled carbon nanotubes provided specific extraction capability for psoralen and isopsoralen.
28715109	6	27	theme	low	742:744	arg1	limits					746:751	low limits	742:751	low limits of detection (20 pg/mL, S/N = 3) for both psoralen and isopsoralen	742:818	The method showed low limits of detection (20 pg/mL, S/N = 3) for both psoralen and isopsoralen.
28715109	0	28	theme	Polymeric	0:8	arg1	column					19:24	Polymeric monolith column	0:24	Polymeric monolith column	0:24	Polymeric monolith column composited with multiwalled carbon nanotubes-β-cyclodextrin for the selective extraction of psoralen and isopsoralen.
28715109	8	29	theme	carbon	1102:1107	arg1	nanotubes					1109:1117	multiwalled carbon nanotubes	1090:1117	multiwalled carbon nanotubes	1090:1117	The empty hydrophobic cavities of β-cyclodextrin and the hydrophobicity of multiwalled carbon nanotubes provided specific extraction capability for psoralen and isopsoralen.
28715109	6	30	dep	detection	756:764	arg1	S/N = 3					777:783	S/N = 3	777:783	S/N = 3	777:783	The method showed low limits of detection (20 pg/mL, S/N = 3) for both psoralen and isopsoralen.
28715109	6	30	dep	detection	756:764	arg1	20 pg/mL					767:774	20 pg/mL	767:774	20 pg/mL	767:774	The method showed low limits of detection (20 pg/mL, S/N = 3) for both psoralen and isopsoralen.
28715109	8	31	theme	multiwalled	1090:1100	arg1	nanotubes					1109:1117	multiwalled carbon nanotubes	1090:1117	multiwalled carbon nanotubes	1090:1117	The empty hydrophobic cavities of β-cyclodextrin and the hydrophobicity of multiwalled carbon nanotubes provided specific extraction capability for psoralen and isopsoralen.
28715109	3	32	theme	isopsoralen	467:477	arg1	microextraction					435:449	the online solid-phase microextraction	412:449	the online solid-phase microextraction of psoralen and isopsoralen from Fructus Psoraleae	412:500	The column was then applied for the online solid-phase microextraction of psoralen and isopsoralen from Fructus Psoraleae.
28715109	8	33	theme	specific	1128:1135	arg1	capability					1148:1157	specific extraction capability	1128:1157	specific extraction capability for psoralen and isopsoralen	1128:1186	The empty hydrophobic cavities of β-cyclodextrin and the hydrophobicity of multiwalled carbon nanotubes provided specific extraction capability for psoralen and isopsoralen.
28715109	2	34	theme	0.76 mm	347:353	arg1	id					355:356	0.76 mm id	347:356	0.76 mm id	347:356	The composite was wrapped into the poly(butyl methacrylate-ethylene dimethacrylate) monolith column (0.76 mm id and 10 cm in length).
28715109	0	35	theme	multiwalled	42:52	arg1	nanotubes-β-cyclodextrin					61:84	multiwalled carbon nanotubes-β-cyclodextrin	42:84	multiwalled carbon nanotubes-β-cyclodextrin for the selective extraction of psoralen and isopsoralen	42:141	Polymeric monolith column composited with multiwalled carbon nanotubes-β-cyclodextrin for the selective extraction of psoralen and isopsoralen.
28715109	4	36	theme	UV	633:634	arg1	detection					636:644	UV detection	633:644	UV detection	633:644	Following microextraction, the coumarins were quantified by high-performance liquid chromatography with C18 separation column and UV detection.
28715109	2	37	theme	poly	281:284	arg1	column					339:344	the poly(butyl methacrylate-ethylene dimethacrylate) monolith column	277:344	the poly(butyl methacrylate-ethylene dimethacrylate) monolith column (0.76 mm id and 10 cm in length)	277:377	The composite was wrapped into the poly(butyl methacrylate-ethylene dimethacrylate) monolith column (0.76 mm id and 10 cm in length).
28715109	3	38	theme	psoralen	454:461	arg1	microextraction					435:449	the online solid-phase microextraction	412:449	the online solid-phase microextraction of psoralen and isopsoralen from Fructus Psoraleae	412:500	The column was then applied for the online solid-phase microextraction of psoralen and isopsoralen from Fructus Psoraleae.
28715109	8	39	theme	extraction	1137:1146	arg1	capability					1148:1157	specific extraction capability	1128:1157	specific extraction capability for psoralen and isopsoralen	1128:1186	The empty hydrophobic cavities of β-cyclodextrin and the hydrophobicity of multiwalled carbon nanotubes provided specific extraction capability for psoralen and isopsoralen.
28715109	3	40	from	Psoraleae	492:500	arg1	microextraction					435:449	the online solid-phase microextraction	412:449	the online solid-phase microextraction of psoralen and isopsoralen from Fructus Psoraleae	412:500	The column was then applied for the online solid-phase microextraction of psoralen and isopsoralen from Fructus Psoraleae.
28715109	3	41	theme	solid-phase	423:433	arg1	microextraction					435:449	the online solid-phase microextraction	412:449	the online solid-phase microextraction of psoralen and isopsoralen from Fructus Psoraleae	412:500	The column was then applied for the online solid-phase microextraction of psoralen and isopsoralen from Fructus Psoraleae.
28715109	0	42	theme	carbon	54:59	arg1	nanotubes-β-cyclodextrin					61:84	multiwalled carbon nanotubes-β-cyclodextrin	42:84	multiwalled carbon nanotubes-β-cyclodextrin for the selective extraction of psoralen and isopsoralen	42:141	Polymeric monolith column composited with multiwalled carbon nanotubes-β-cyclodextrin for the selective extraction of psoralen and isopsoralen.
28715109	4	43	theme	separation	611:620	arg1	column					622:627	C18 separation column	607:627	C18 separation column	607:627	Following microextraction, the coumarins were quantified by high-performance liquid chromatography with C18 separation column and UV detection.
28715109	4	44	theme	C18	607:609	arg1	column					622:627	C18 separation column	607:627	C18 separation column	607:627	Following microextraction, the coumarins were quantified by high-performance liquid chromatography with C18 separation column and UV detection.
28715109	1	45	theme	polymeric	146:154	arg1	column					156:161	A polymeric column	144:161	A polymeric column that contains multiwalled carbon nanotubes-β-cyclodextrin composite	144:229	A polymeric column that contains multiwalled carbon nanotubes-β-cyclodextrin composite was developed.
28715109	2	46	theme	methacrylate-ethylene	292:312	arg1	poly					281:284	poly	281:284	the poly(butyl methacrylate-ethylene dimethacrylate) monolith column (0.76 mm id and 10 cm in length)	277:377	The composite was wrapped into the poly(butyl methacrylate-ethylene dimethacrylate) monolith column (0.76 mm id and 10 cm in length).
28715109	2	46	theme	methacrylate-ethylene	292:312	arg1	dimethacrylate					314:327	butyl methacrylate-ethylene dimethacrylate	286:327	butyl methacrylate-ethylene dimethacrylate	286:327	The composite was wrapped into the poly(butyl methacrylate-ethylene dimethacrylate) monolith column (0.76 mm id and 10 cm in length).
28715109	7	47	theme	spiked	917:922	arg1	extracts					929:936	spiked herb extracts	917:936	spiked herb extracts	917:936	Finally the method was successfully applied to the determination of psoralen and isopsoralen in spiked herb extracts and rat plasma where it gave recoveries that ranged between 93.2 and 102.1%.
28715109	2	48	theme	butyl	286:290	arg1	poly					281:284	poly	281:284	the poly(butyl methacrylate-ethylene dimethacrylate) monolith column (0.76 mm id and 10 cm in length)	277:377	The composite was wrapped into the poly(butyl methacrylate-ethylene dimethacrylate) monolith column (0.76 mm id and 10 cm in length).
28715109	2	48	theme	butyl	286:290	arg1	dimethacrylate					314:327	butyl methacrylate-ethylene dimethacrylate	286:327	butyl methacrylate-ethylene dimethacrylate	286:327	The composite was wrapped into the poly(butyl methacrylate-ethylene dimethacrylate) monolith column (0.76 mm id and 10 cm in length).
28715109	7	49	theme	herb	924:927	arg1	extracts					929:936	spiked herb extracts	917:936	spiked herb extracts	917:936	Finally the method was successfully applied to the determination of psoralen and isopsoralen in spiked herb extracts and rat plasma where it gave recoveries that ranged between 93.2 and 102.1%.
28715109	7	50	theme	rat	942:944	arg1	plasma					946:951	rat plasma	942:951	rat plasma	942:951	Finally the method was successfully applied to the determination of psoralen and isopsoralen in spiked herb extracts and rat plasma where it gave recoveries that ranged between 93.2 and 102.1%.
28715109	8	51	theme	β-cyclodextrin	1049:1062	arg1	cavities					1037:1044	The empty hydrophobic cavities	1015:1044	The empty hydrophobic cavities of β-cyclodextrin and the hydrophobicity of multiwalled carbon nanotubes	1015:1117	The empty hydrophobic cavities of β-cyclodextrin and the hydrophobicity of multiwalled carbon nanotubes provided specific extraction capability for psoralen and isopsoralen.
28715109	8	52	theme	nanotubes	1109:1117	arg1	β-cyclodextrin					1049:1062	β-cyclodextrin	1049:1062	β-cyclodextrin	1049:1062	The empty hydrophobic cavities of β-cyclodextrin and the hydrophobicity of multiwalled carbon nanotubes provided specific extraction capability for psoralen and isopsoralen.
28715109	8	52	theme	nanotubes	1109:1117	arg1	hydrophobicity					1072:1085	the hydrophobicity	1068:1085	the hydrophobicity of multiwalled carbon nanotubes	1068:1117	The empty hydrophobic cavities of β-cyclodextrin and the hydrophobicity of multiwalled carbon nanotubes provided specific extraction capability for psoralen and isopsoralen.
28715109	2	53	from	id	355:356	arg1	length					371:376	length	371:376	length	371:376	The composite was wrapped into the poly(butyl methacrylate-ethylene dimethacrylate) monolith column (0.76 mm id and 10 cm in length).
24842272	7	0	gly	glycopeptides	1342:1354	arg2	glycopeptides					1342:1354	type-1 mucin glycopeptides	1329:1354	type-1 mucin glycopeptides	1329:1354	The obtained glycosylated 9-fluorenylmethoxycarbonyl (Fmoc)-protected amino acid building blocks were employed in synthesis of type-1 mucin glycopeptides, which are useful in biological applications.
24842272	7	1	from	useful	1367:1372	arg1	applications					1388:1399	biological applications	1377:1399	biological applications	1377:1399	The obtained glycosylated 9-fluorenylmethoxycarbonyl (Fmoc)-protected amino acid building blocks were employed in synthesis of type-1 mucin glycopeptides, which are useful in biological applications.
24842272	6	2	theme	straightforward	1090:1104	arg1	preparation					1106:1116	straightforward preparation	1090:1116	straightforward preparation of diverse glycosylated amino acids carrying the type-1 mucin core 1-3 saccharides	1090:1199	By utilizing differentiation in acceptor reactivity, shared early stage Tn- and T-acceptor intermediates were elongated with a common type-1 [β-D-Gal-1,3-β-D-GlcNAc] disaccharide, which allows for straightforward preparation of diverse glycosylated amino acids carrying the type-1 mucin core 1-3 saccharides.
24842272	6	3	contain	carrying	1154:1161	arg2	saccharides					1189:1199	the type-1 mucin core 1-3 saccharides	1163:1199	the type-1 mucin core 1-3 saccharides	1163:1199	By utilizing differentiation in acceptor reactivity, shared early stage Tn- and T-acceptor intermediates were elongated with a common type-1 [β-D-Gal-1,3-β-D-GlcNAc] disaccharide, which allows for straightforward preparation of diverse glycosylated amino acids carrying the type-1 mucin core 1-3 saccharides.
24842272	6	3	contain	carrying	1154:1161	arg1	acids					1148:1152	diverse glycosylated amino acids	1121:1152	diverse glycosylated amino acids carrying the type-1 mucin core 1-3 saccharides	1121:1199	By utilizing differentiation in acceptor reactivity, shared early stage Tn- and T-acceptor intermediates were elongated with a common type-1 [β-D-Gal-1,3-β-D-GlcNAc] disaccharide, which allows for straightforward preparation of diverse glycosylated amino acids carrying the type-1 mucin core 1-3 saccharides.
24842272	7	4	theme	Fmoc	1256:1259	arg1	blocks					1292:1297	The obtained glycosylated 9-fluorenylmethoxycarbonyl (Fmoc)-protected amino acid building blocks	1202:1297	The obtained glycosylated 9-fluorenylmethoxycarbonyl (Fmoc)-protected amino acid building blocks	1202:1297	The obtained glycosylated 9-fluorenylmethoxycarbonyl (Fmoc)-protected amino acid building blocks were employed in synthesis of type-1 mucin glycopeptides, which are useful in biological applications.
24842272	3	5	from	interactions	382:393	arg1	conditions					482:491	morbidly altered or unaltered conditions	452:491	morbidly altered or unaltered conditions	452:491	With that in mind, the elucidation of interactions of carbohydrate binding proteins with mucins, whether in morbidly altered or unaltered conditions, continue to shed light on mechanisms involved in diseases like chronic inflammations and cancer.
24842272	3	6	from	conditions	482:491	arg1	elucidation					367:377	the elucidation	363:377	the elucidation of interactions of carbohydrate binding proteins with mucins, whether in morbidly altered or unaltered conditions,	363:492	With that in mind, the elucidation of interactions of carbohydrate binding proteins with mucins, whether in morbidly altered or unaltered conditions, continue to shed light on mechanisms involved in diseases like chronic inflammations and cancer.
24842272	1	7	theme	O-glycosylated	142:155	arg1	proteins					157:164	highly O-glycosylated proteins	135:164	highly O-glycosylated proteins found on the surface of cells in epithelial tissues	135:216	Mucins are a class of highly O-glycosylated proteins found on the surface of cells in epithelial tissues.
24842272	6	8	theme	T-acceptor	973:982	arg1	intermediates					984:996	shared early stage Tn- and T-acceptor intermediates	946:996	intermediates	984:996	By utilizing differentiation in acceptor reactivity, shared early stage Tn- and T-acceptor intermediates were elongated with a common type-1 [β-D-Gal-1,3-β-D-GlcNAc] disaccharide, which allows for straightforward preparation of diverse glycosylated amino acids carrying the type-1 mucin core 1-3 saccharides.
24842272	7	9	theme	glycosylated	1215:1226	arg1	blocks					1292:1297	The obtained glycosylated 9-fluorenylmethoxycarbonyl (Fmoc)-protected amino acid building blocks	1202:1297	The obtained glycosylated 9-fluorenylmethoxycarbonyl (Fmoc)-protected amino acid building blocks	1202:1297	The obtained glycosylated 9-fluorenylmethoxycarbonyl (Fmoc)-protected amino acid building blocks were employed in synthesis of type-1 mucin glycopeptides, which are useful in biological applications.
24842272	7	10	theme	glycopeptides	1342:1354	arg1	synthesis					1316:1324	synthesis	1316:1324	synthesis of type-1 mucin glycopeptides, which are useful in biological applications	1316:1399	The obtained glycosylated 9-fluorenylmethoxycarbonyl (Fmoc)-protected amino acid building blocks were employed in synthesis of type-1 mucin glycopeptides, which are useful in biological applications.
24842272	4	11	theme	elongated	641:649	arg1	cores					657:661	type-2 elongated mucin cores 1-4	634:665	type-2 elongated mucin cores 1-4	634:665	Despite the known importance of type-1 and type-2 elongated mucin cores 1-4 in glycobiology, the corresponding type-1 structures are much less well studied.
24842272	7	12	theme	9-fluorenylmethoxycarbonyl	1228:1253	arg1	blocks					1292:1297	The obtained glycosylated 9-fluorenylmethoxycarbonyl (Fmoc)-protected amino acid building blocks	1202:1297	The obtained glycosylated 9-fluorenylmethoxycarbonyl (Fmoc)-protected amino acid building blocks	1202:1297	The obtained glycosylated 9-fluorenylmethoxycarbonyl (Fmoc)-protected amino acid building blocks were employed in synthesis of type-1 mucin glycopeptides, which are useful in biological applications.
24842272	7	13	gly	glycosylated	1215:1226	arg1	blocks					1292:1297	The obtained glycosylated 9-fluorenylmethoxycarbonyl (Fmoc)-protected amino acid building blocks	1202:1297	The obtained glycosylated 9-fluorenylmethoxycarbonyl (Fmoc)-protected amino acid building blocks	1202:1297	The obtained glycosylated 9-fluorenylmethoxycarbonyl (Fmoc)-protected amino acid building blocks were employed in synthesis of type-1 mucin glycopeptides, which are useful in biological applications.
24842272	7	14	theme	acid	1278:1281	arg1	blocks					1292:1297	The obtained glycosylated 9-fluorenylmethoxycarbonyl (Fmoc)-protected amino acid building blocks	1202:1297	The obtained glycosylated 9-fluorenylmethoxycarbonyl (Fmoc)-protected amino acid building blocks	1202:1297	The obtained glycosylated 9-fluorenylmethoxycarbonyl (Fmoc)-protected amino acid building blocks were employed in synthesis of type-1 mucin glycopeptides, which are useful in biological applications.
24842272	2	15	theme	mucins	271:276	arg1	functionality					254:266	the functionality	250:266	the functionality of mucins	250:276	O-Glycosylation is crucial for the functionality of mucins and changes therein can have severe consequences for an organism.
24842272	3	16	theme	altered	461:467	arg1	conditions					482:491	morbidly altered or unaltered conditions	452:491	morbidly altered or unaltered conditions	452:491	With that in mind, the elucidation of interactions of carbohydrate binding proteins with mucins, whether in morbidly altered or unaltered conditions, continue to shed light on mechanisms involved in diseases like chronic inflammations and cancer.
24842272	6	17	theme	acids	1148:1152	arg1	preparation					1106:1116	straightforward preparation	1090:1116	straightforward preparation of diverse glycosylated amino acids carrying the type-1 mucin core 1-3 saccharides	1090:1199	By utilizing differentiation in acceptor reactivity, shared early stage Tn- and T-acceptor intermediates were elongated with a common type-1 [β-D-Gal-1,3-β-D-GlcNAc] disaccharide, which allows for straightforward preparation of diverse glycosylated amino acids carrying the type-1 mucin core 1-3 saccharides.
24842272	6	18	theme	[β-D-Gal-1,3-β-D-GlcNAc	1034:1056	arg1	disaccharide					1059:1070	a common type-1 [β-D-Gal-1,3-β-D-GlcNAc] disaccharide	1018:1070	a common type-1 [β-D-Gal-1,3-β-D-GlcNAc] disaccharide	1018:1070	By utilizing differentiation in acceptor reactivity, shared early stage Tn- and T-acceptor intermediates were elongated with a common type-1 [β-D-Gal-1,3-β-D-GlcNAc] disaccharide, which allows for straightforward preparation of diverse glycosylated amino acids carrying the type-1 mucin core 1-3 saccharides.
24842272	3	19	with	interactions	382:393	arg1	mucins					433:438	mucins	433:438	mucins	433:438	With that in mind, the elucidation of interactions of carbohydrate binding proteins with mucins, whether in morbidly altered or unaltered conditions, continue to shed light on mechanisms involved in diseases like chronic inflammations and cancer.
24842272	4	20	theme	cores	657:661	arg1	importance					609:618	the known importance	599:618	the known importance of type-1 and type-2 elongated mucin cores 1-4 in glycobiology	599:681	Despite the known importance of type-1 and type-2 elongated mucin cores 1-4 in glycobiology, the corresponding type-1 structures are much less well studied.
24842272	3	21	theme	proteins	419:426	arg1	interactions					382:393	interactions	382:393	interactions of carbohydrate binding proteins with mucins, whether in morbidly altered or unaltered conditions,	382:492	With that in mind, the elucidation of interactions of carbohydrate binding proteins with mucins, whether in morbidly altered or unaltered conditions, continue to shed light on mechanisms involved in diseases like chronic inflammations and cancer.
24842272	0	22	theme	corresponding	84:96	arg1	glycopeptides					98:110	the corresponding glycopeptides	80:110	the corresponding glycopeptides	80:110	A convergent strategy for the synthesis of type-1 elongated mucin cores 1-3 and the corresponding glycopeptides.
24842272	6	23	theme	core	1180:1183	arg1	saccharides					1189:1199	the type-1 mucin core 1-3 saccharides	1163:1199	the type-1 mucin core 1-3 saccharides	1163:1199	By utilizing differentiation in acceptor reactivity, shared early stage Tn- and T-acceptor intermediates were elongated with a common type-1 [β-D-Gal-1,3-β-D-GlcNAc] disaccharide, which allows for straightforward preparation of diverse glycosylated amino acids carrying the type-1 mucin core 1-3 saccharides.
24842272	7	24	from	applications	1388:1399	arg1	useful					1367:1372	useful	1367:1372	useful	1367:1372	The obtained glycosylated 9-fluorenylmethoxycarbonyl (Fmoc)-protected amino acid building blocks were employed in synthesis of type-1 mucin glycopeptides, which are useful in biological applications.
24842272	4	25	theme	type-2	634:639	arg1	cores					657:661	type-2 elongated mucin cores 1-4	634:665	type-2 elongated mucin cores 1-4	634:665	Despite the known importance of type-1 and type-2 elongated mucin cores 1-4 in glycobiology, the corresponding type-1 structures are much less well studied.
24842272	0	26	theme	convergent	2:11	arg1	strategy					13:20	A convergent strategy	0:20	A convergent strategy for the synthesis of type-1	0:48	A convergent strategy for the synthesis of type-1 elongated mucin cores 1-3 and the corresponding glycopeptides.
24842272	6	27	gly	glycosylated	1129:1140	arg1	acids					1148:1152	diverse glycosylated amino acids	1121:1152	diverse glycosylated amino acids carrying the type-1 mucin core 1-3 saccharides	1121:1199	By utilizing differentiation in acceptor reactivity, shared early stage Tn- and T-acceptor intermediates were elongated with a common type-1 [β-D-Gal-1,3-β-D-GlcNAc] disaccharide, which allows for straightforward preparation of diverse glycosylated amino acids carrying the type-1 mucin core 1-3 saccharides.
24842272	6	28	theme	mucin	1174:1178	arg1	saccharides					1189:1199	the type-1 mucin core 1-3 saccharides	1163:1199	the type-1 mucin core 1-3 saccharides	1163:1199	By utilizing differentiation in acceptor reactivity, shared early stage Tn- and T-acceptor intermediates were elongated with a common type-1 [β-D-Gal-1,3-β-D-GlcNAc] disaccharide, which allows for straightforward preparation of diverse glycosylated amino acids carrying the type-1 mucin core 1-3 saccharides.
24842272	3	29	theme	carbohydrate	398:409	arg1	proteins					419:426	carbohydrate binding proteins	398:426	carbohydrate binding proteins	398:426	With that in mind, the elucidation of interactions of carbohydrate binding proteins with mucins, whether in morbidly altered or unaltered conditions, continue to shed light on mechanisms involved in diseases like chronic inflammations and cancer.
24842272	5	30	theme	extended	786:793	arg1	structures					837:846	extended mucin type-1 O-glycan core 1-3 amino acid structures	786:846	extended mucin type-1 O-glycan core 1-3 amino acid structures based on a convergent approach	786:877	Here, the first chemical synthesis of extended mucin type-1 O-glycan core 1-3 amino acid structures based on a convergent approach is presented.
24842272	6	31	theme	stage	959:963	arg1	Tn-					965:967	shared early stage Tn- and T-acceptor intermediates	946:996	Tn-	965:967	By utilizing differentiation in acceptor reactivity, shared early stage Tn- and T-acceptor intermediates were elongated with a common type-1 [β-D-Gal-1,3-β-D-GlcNAc] disaccharide, which allows for straightforward preparation of diverse glycosylated amino acids carrying the type-1 mucin core 1-3 saccharides.
24842272	4	32	theme	type-1	702:707	arg1	structures					709:718	the corresponding type-1 structures	684:718	the corresponding type-1 structures	684:718	Despite the known importance of type-1 and type-2 elongated mucin cores 1-4 in glycobiology, the corresponding type-1 structures are much less well studied.
24842272	5	33	theme	acid	832:835	arg1	structures					837:846	extended mucin type-1 O-glycan core 1-3 amino acid structures	786:846	extended mucin type-1 O-glycan core 1-3 amino acid structures based on a convergent approach	786:877	Here, the first chemical synthesis of extended mucin type-1 O-glycan core 1-3 amino acid structures based on a convergent approach is presented.
24842272	6	34	theme	type-1	1167:1172	arg1	saccharides					1189:1199	the type-1 mucin core 1-3 saccharides	1163:1199	the type-1 mucin core 1-3 saccharides	1163:1199	By utilizing differentiation in acceptor reactivity, shared early stage Tn- and T-acceptor intermediates were elongated with a common type-1 [β-D-Gal-1,3-β-D-GlcNAc] disaccharide, which allows for straightforward preparation of diverse glycosylated amino acids carrying the type-1 mucin core 1-3 saccharides.
24842272	7	35	theme	type-1	1329:1334	arg1	glycopeptides					1342:1354	type-1 mucin glycopeptides	1329:1354	type-1 mucin glycopeptides	1329:1354	The obtained glycosylated 9-fluorenylmethoxycarbonyl (Fmoc)-protected amino acid building blocks were employed in synthesis of type-1 mucin glycopeptides, which are useful in biological applications.
24842272	3	36	theme	binding	411:417	arg1	proteins					419:426	carbohydrate binding proteins	398:426	carbohydrate binding proteins	398:426	With that in mind, the elucidation of interactions of carbohydrate binding proteins with mucins, whether in morbidly altered or unaltered conditions, continue to shed light on mechanisms involved in diseases like chronic inflammations and cancer.
24842272	5	37	theme	mucin	795:799	arg1	structures					837:846	extended mucin type-1 O-glycan core 1-3 amino acid structures	786:846	extended mucin type-1 O-glycan core 1-3 amino acid structures based on a convergent approach	786:877	Here, the first chemical synthesis of extended mucin type-1 O-glycan core 1-3 amino acid structures based on a convergent approach is presented.
24842272	6	38	theme	early	953:957	arg1	Tn-					965:967	shared early stage Tn- and T-acceptor intermediates	946:996	Tn-	965:967	By utilizing differentiation in acceptor reactivity, shared early stage Tn- and T-acceptor intermediates were elongated with a common type-1 [β-D-Gal-1,3-β-D-GlcNAc] disaccharide, which allows for straightforward preparation of diverse glycosylated amino acids carrying the type-1 mucin core 1-3 saccharides.
24842272	5	39	theme	O-glycan	808:815	arg1	structures					837:846	extended mucin type-1 O-glycan core 1-3 amino acid structures	786:846	extended mucin type-1 O-glycan core 1-3 amino acid structures based on a convergent approach	786:877	Here, the first chemical synthesis of extended mucin type-1 O-glycan core 1-3 amino acid structures based on a convergent approach is presented.
24842272	5	40	theme	type-1	801:806	arg1	structures					837:846	extended mucin type-1 O-glycan core 1-3 amino acid structures	786:846	extended mucin type-1 O-glycan core 1-3 amino acid structures based on a convergent approach	786:877	Here, the first chemical synthesis of extended mucin type-1 O-glycan core 1-3 amino acid structures based on a convergent approach is presented.
24842272	6	41	theme	shared	946:951	arg1	Tn-					965:967	shared early stage Tn- and T-acceptor intermediates	946:996	Tn-	965:967	By utilizing differentiation in acceptor reactivity, shared early stage Tn- and T-acceptor intermediates were elongated with a common type-1 [β-D-Gal-1,3-β-D-GlcNAc] disaccharide, which allows for straightforward preparation of diverse glycosylated amino acids carrying the type-1 mucin core 1-3 saccharides.
24842272	1	42	located	found	166:170	arg2	proteins					157:164	highly O-glycosylated proteins	135:164	highly O-glycosylated proteins found on the surface of cells in epithelial tissues	135:216	Mucins are a class of highly O-glycosylated proteins found on the surface of cells in epithelial tissues.
24842272	1	42	located	found	166:170	arg1	surface					179:185	the surface	175:185	the surface of cells in epithelial tissues	175:216	Mucins are a class of highly O-glycosylated proteins found on the surface of cells in epithelial tissues.
24842272	0	43	gly	glycopeptides	98:110	arg2	glycopeptides					98:110	the corresponding glycopeptides	80:110	the corresponding glycopeptides	80:110	A convergent strategy for the synthesis of type-1 elongated mucin cores 1-3 and the corresponding glycopeptides.
24842272	5	44	theme	core	817:820	arg1	structures					837:846	extended mucin type-1 O-glycan core 1-3 amino acid structures	786:846	extended mucin type-1 O-glycan core 1-3 amino acid structures based on a convergent approach	786:877	Here, the first chemical synthesis of extended mucin type-1 O-glycan core 1-3 amino acid structures based on a convergent approach is presented.
24842272	3	45	theme	interactions	382:393	arg1	elucidation					367:377	the elucidation	363:377	the elucidation of interactions of carbohydrate binding proteins with mucins, whether in morbidly altered or unaltered conditions,	363:492	With that in mind, the elucidation of interactions of carbohydrate binding proteins with mucins, whether in morbidly altered or unaltered conditions, continue to shed light on mechanisms involved in diseases like chronic inflammations and cancer.
24842272	6	46	theme	amino	1142:1146	arg1	acids					1148:1152	diverse glycosylated amino acids	1121:1152	diverse glycosylated amino acids carrying the type-1 mucin core 1-3 saccharides	1121:1199	By utilizing differentiation in acceptor reactivity, shared early stage Tn- and T-acceptor intermediates were elongated with a common type-1 [β-D-Gal-1,3-β-D-GlcNAc] disaccharide, which allows for straightforward preparation of diverse glycosylated amino acids carrying the type-1 mucin core 1-3 saccharides.
24842272	6	47	theme	type-1	1027:1032	arg1	disaccharide					1059:1070	a common type-1 [β-D-Gal-1,3-β-D-GlcNAc] disaccharide	1018:1070	a common type-1 [β-D-Gal-1,3-β-D-GlcNAc] disaccharide	1018:1070	By utilizing differentiation in acceptor reactivity, shared early stage Tn- and T-acceptor intermediates were elongated with a common type-1 [β-D-Gal-1,3-β-D-GlcNAc] disaccharide, which allows for straightforward preparation of diverse glycosylated amino acids carrying the type-1 mucin core 1-3 saccharides.
24842272	4	48	theme	mucin	651:655	arg1	cores					657:661	type-2 elongated mucin cores 1-4	634:665	type-2 elongated mucin cores 1-4	634:665	Despite the known importance of type-1 and type-2 elongated mucin cores 1-4 in glycobiology, the corresponding type-1 structures are much less well studied.
24842272	1	49	theme	cells	190:194	arg1	surface					179:185	the surface	175:185	the surface of cells in epithelial tissues	175:216	Mucins are a class of highly O-glycosylated proteins found on the surface of cells in epithelial tissues.
24842272	1	50	theme	proteins	157:164	arg1	Mucins					113:118	Mucins	113:118	Mucins	113:118	Mucins are a class of highly O-glycosylated proteins found on the surface of cells in epithelial tissues.
24842272	1	50	theme	proteins	157:164	arg1	class					126:130	a class	124:130	a class of highly O-glycosylated proteins found on the surface of cells in epithelial tissues	124:216	Mucins are a class of highly O-glycosylated proteins found on the surface of cells in epithelial tissues.
24842272	5	51	theme	first	758:762	arg1	synthesis					773:781	the first chemical synthesis	754:781	the first chemical synthesis of extended mucin type-1 O-glycan core 1-3 amino acid structures based on a convergent approach	754:877	Here, the first chemical synthesis of extended mucin type-1 O-glycan core 1-3 amino acid structures based on a convergent approach is presented.
24842272	7	52	theme	obtained	1206:1213	arg1	blocks					1292:1297	The obtained glycosylated 9-fluorenylmethoxycarbonyl (Fmoc)-protected amino acid building blocks	1202:1297	The obtained glycosylated 9-fluorenylmethoxycarbonyl (Fmoc)-protected amino acid building blocks	1202:1297	The obtained glycosylated 9-fluorenylmethoxycarbonyl (Fmoc)-protected amino acid building blocks were employed in synthesis of type-1 mucin glycopeptides, which are useful in biological applications.
24842272	1	53	from	cells	190:194	arg1	tissues					210:216	epithelial tissues	199:216	epithelial tissues	199:216	Mucins are a class of highly O-glycosylated proteins found on the surface of cells in epithelial tissues.
24842272	4	54	theme	type-1	623:628	arg1	importance					609:618	the known importance	599:618	the known importance of type-1 and type-2 elongated mucin cores 1-4 in glycobiology	599:681	Despite the known importance of type-1 and type-2 elongated mucin cores 1-4 in glycobiology, the corresponding type-1 structures are much less well studied.
24842272	0	55	theme	type-1	43:48	arg1	synthesis					30:38	the synthesis	26:38	the synthesis of type-1	26:48	A convergent strategy for the synthesis of type-1 elongated mucin cores 1-3 and the corresponding glycopeptides.
24842272	6	56	theme	acceptor	925:932	arg1	reactivity					934:943	acceptor reactivity	925:943	acceptor reactivity	925:943	By utilizing differentiation in acceptor reactivity, shared early stage Tn- and T-acceptor intermediates were elongated with a common type-1 [β-D-Gal-1,3-β-D-GlcNAc] disaccharide, which allows for straightforward preparation of diverse glycosylated amino acids carrying the type-1 mucin core 1-3 saccharides.
24842272	6	57	theme	common	1020:1025	arg1	disaccharide					1059:1070	a common type-1 [β-D-Gal-1,3-β-D-GlcNAc] disaccharide	1018:1070	a common type-1 [β-D-Gal-1,3-β-D-GlcNAc] disaccharide	1018:1070	By utilizing differentiation in acceptor reactivity, shared early stage Tn- and T-acceptor intermediates were elongated with a common type-1 [β-D-Gal-1,3-β-D-GlcNAc] disaccharide, which allows for straightforward preparation of diverse glycosylated amino acids carrying the type-1 mucin core 1-3 saccharides.
24842272	7	58	theme	-protected	1261:1270	arg1	blocks					1292:1297	The obtained glycosylated 9-fluorenylmethoxycarbonyl (Fmoc)-protected amino acid building blocks	1202:1297	The obtained glycosylated 9-fluorenylmethoxycarbonyl (Fmoc)-protected amino acid building blocks	1202:1297	The obtained glycosylated 9-fluorenylmethoxycarbonyl (Fmoc)-protected amino acid building blocks were employed in synthesis of type-1 mucin glycopeptides, which are useful in biological applications.
24842272	3	59	theme	chronic	557:563	arg1	inflammations					565:577	chronic inflammations	557:577	chronic inflammations	557:577	With that in mind, the elucidation of interactions of carbohydrate binding proteins with mucins, whether in morbidly altered or unaltered conditions, continue to shed light on mechanisms involved in diseases like chronic inflammations and cancer.
24842272	4	60	theme	corresponding	688:700	arg1	structures					709:718	the corresponding type-1 structures	684:718	the corresponding type-1 structures	684:718	Despite the known importance of type-1 and type-2 elongated mucin cores 1-4 in glycobiology, the corresponding type-1 structures are much less well studied.
24842272	6	61	from	differentiation	906:920	arg1	reactivity					934:943	acceptor reactivity	925:943	acceptor reactivity	925:943	By utilizing differentiation in acceptor reactivity, shared early stage Tn- and T-acceptor intermediates were elongated with a common type-1 [β-D-Gal-1,3-β-D-GlcNAc] disaccharide, which allows for straightforward preparation of diverse glycosylated amino acids carrying the type-1 mucin core 1-3 saccharides.
24842272	1	62	from	surface	179:185	arg1	tissues					210:216	epithelial tissues	199:216	epithelial tissues	199:216	Mucins are a class of highly O-glycosylated proteins found on the surface of cells in epithelial tissues.
24842272	3	63	theme	unaltered	472:480	arg1	conditions					482:491	morbidly altered or unaltered conditions	452:491	morbidly altered or unaltered conditions	452:491	With that in mind, the elucidation of interactions of carbohydrate binding proteins with mucins, whether in morbidly altered or unaltered conditions, continue to shed light on mechanisms involved in diseases like chronic inflammations and cancer.
24842272	2	64	contain	have	302:305	arg1	therein					290:296	therein	290:296	therein	290:296	O-Glycosylation is crucial for the functionality of mucins and changes therein can have severe consequences for an organism.
24842272	2	64	contain	have	302:305	arg2	consequences					314:325	severe consequences	307:325	severe consequences	307:325	O-Glycosylation is crucial for the functionality of mucins and changes therein can have severe consequences for an organism.
24842272	1	65	gly	O-glycosylated	142:155	arg1	proteins					157:164	highly O-glycosylated proteins	135:164	highly O-glycosylated proteins found on the surface of cells in epithelial tissues	135:216	Mucins are a class of highly O-glycosylated proteins found on the surface of cells in epithelial tissues.
24842272	4	66	dep	cores	657:661	arg1	1-4					663:665	1-4	663:665	1-4	663:665	Despite the known importance of type-1 and type-2 elongated mucin cores 1-4 in glycobiology, the corresponding type-1 structures are much less well studied.
24842272	6	67	theme	glycosylated	1129:1140	arg1	acids					1148:1152	diverse glycosylated amino acids	1121:1152	diverse glycosylated amino acids carrying the type-1 mucin core 1-3 saccharides	1121:1199	By utilizing differentiation in acceptor reactivity, shared early stage Tn- and T-acceptor intermediates were elongated with a common type-1 [β-D-Gal-1,3-β-D-GlcNAc] disaccharide, which allows for straightforward preparation of diverse glycosylated amino acids carrying the type-1 mucin core 1-3 saccharides.
24842272	6	68	theme	diverse	1121:1127	arg1	acids					1148:1152	diverse glycosylated amino acids	1121:1152	diverse glycosylated amino acids carrying the type-1 mucin core 1-3 saccharides	1121:1199	By utilizing differentiation in acceptor reactivity, shared early stage Tn- and T-acceptor intermediates were elongated with a common type-1 [β-D-Gal-1,3-β-D-GlcNAc] disaccharide, which allows for straightforward preparation of diverse glycosylated amino acids carrying the type-1 mucin core 1-3 saccharides.
24842272	1	69	theme	epithelial	199:208	arg1	tissues					210:216	epithelial tissues	199:216	epithelial tissues	199:216	Mucins are a class of highly O-glycosylated proteins found on the surface of cells in epithelial tissues.
24842272	1	70	from	tissues	210:216	arg1	surface					179:185	the surface	175:185	the surface of cells in epithelial tissues	175:216	Mucins are a class of highly O-glycosylated proteins found on the surface of cells in epithelial tissues.
24842272	4	71	from	importance	609:618	arg1	glycobiology					670:681	glycobiology	670:681	glycobiology	670:681	Despite the known importance of type-1 and type-2 elongated mucin cores 1-4 in glycobiology, the corresponding type-1 structures are much less well studied.
24842272	2	72	theme	severe	307:312	arg1	consequences					314:325	severe consequences	307:325	severe consequences	307:325	O-Glycosylation is crucial for the functionality of mucins and changes therein can have severe consequences for an organism.
24842272	0	73	theme	mucin	60:64	arg1	cores					66:70	mucin cores 1-3	60:74	mucin cores 1-3	60:74	A convergent strategy for the synthesis of type-1 elongated mucin cores 1-3 and the corresponding glycopeptides.
24842272	5	74	theme	amino	826:830	arg1	structures					837:846	extended mucin type-1 O-glycan core 1-3 amino acid structures	786:846	extended mucin type-1 O-glycan core 1-3 amino acid structures based on a convergent approach	786:877	Here, the first chemical synthesis of extended mucin type-1 O-glycan core 1-3 amino acid structures based on a convergent approach is presented.
24842272	7	75	theme	mucin	1336:1340	arg1	glycopeptides					1342:1354	type-1 mucin glycopeptides	1329:1354	type-1 mucin glycopeptides	1329:1354	The obtained glycosylated 9-fluorenylmethoxycarbonyl (Fmoc)-protected amino acid building blocks were employed in synthesis of type-1 mucin glycopeptides, which are useful in biological applications.
24842272	7	76	theme	building	1283:1290	arg1	blocks					1292:1297	The obtained glycosylated 9-fluorenylmethoxycarbonyl (Fmoc)-protected amino acid building blocks	1202:1297	The obtained glycosylated 9-fluorenylmethoxycarbonyl (Fmoc)-protected amino acid building blocks	1202:1297	The obtained glycosylated 9-fluorenylmethoxycarbonyl (Fmoc)-protected amino acid building blocks were employed in synthesis of type-1 mucin glycopeptides, which are useful in biological applications.
24842272	5	77	theme	structures	837:846	arg1	synthesis					773:781	the first chemical synthesis	754:781	the first chemical synthesis of extended mucin type-1 O-glycan core 1-3 amino acid structures based on a convergent approach	754:877	Here, the first chemical synthesis of extended mucin type-1 O-glycan core 1-3 amino acid structures based on a convergent approach is presented.
24842272	4	78	theme	known	603:607	arg1	importance					609:618	the known importance	599:618	the known importance of type-1 and type-2 elongated mucin cores 1-4 in glycobiology	599:681	Despite the known importance of type-1 and type-2 elongated mucin cores 1-4 in glycobiology, the corresponding type-1 structures are much less well studied.
24842272	5	79	theme	chemical	764:771	arg1	synthesis					773:781	the first chemical synthesis	754:781	the first chemical synthesis of extended mucin type-1 O-glycan core 1-3 amino acid structures based on a convergent approach	754:877	Here, the first chemical synthesis of extended mucin type-1 O-glycan core 1-3 amino acid structures based on a convergent approach is presented.
24842272	3	80	from	elucidation	367:377	arg1	conditions					482:491	morbidly altered or unaltered conditions	452:491	morbidly altered or unaltered conditions	452:491	With that in mind, the elucidation of interactions of carbohydrate binding proteins with mucins, whether in morbidly altered or unaltered conditions, continue to shed light on mechanisms involved in diseases like chronic inflammations and cancer.
24842272	7	81	theme	biological	1377:1386	arg1	applications					1388:1399	biological applications	1377:1399	biological applications	1377:1399	The obtained glycosylated 9-fluorenylmethoxycarbonyl (Fmoc)-protected amino acid building blocks were employed in synthesis of type-1 mucin glycopeptides, which are useful in biological applications.
24842272	5	82	theme	convergent	859:868	arg1	approach					870:877	a convergent approach	857:877	a convergent approach	857:877	Here, the first chemical synthesis of extended mucin type-1 O-glycan core 1-3 amino acid structures based on a convergent approach is presented.
24842272	7	83	theme	amino	1272:1276	arg1	blocks					1292:1297	The obtained glycosylated 9-fluorenylmethoxycarbonyl (Fmoc)-protected amino acid building blocks	1202:1297	The obtained glycosylated 9-fluorenylmethoxycarbonyl (Fmoc)-protected amino acid building blocks	1202:1297	The obtained glycosylated 9-fluorenylmethoxycarbonyl (Fmoc)-protected amino acid building blocks were employed in synthesis of type-1 mucin glycopeptides, which are useful in biological applications.
26117825	7	0	theme	mass	580:583	arg1	spectrometry					585:596	mass spectrometry	580:596	mass spectrometry	580:596	Sugar and methylation analyses, (1)H and (13)C nuclear magnetic resonance, and mass spectrometry of the NPS or of its specifically modified products allowed determining the repeating unit sequence: {2)Glc(α1-3)Glc(β1-3)[Gal(β1-4)]Gal(β1-4)Gal(α1-}n.
26117825	7	1	theme	NPS	605:607	arg1	spectrometry					585:596	mass spectrometry	580:596	mass spectrometry	580:596	Sugar and methylation analyses, (1)H and (13)C nuclear magnetic resonance, and mass spectrometry of the NPS or of its specifically modified products allowed determining the repeating unit sequence: {2)Glc(α1-3)Glc(β1-3)[Gal(β1-4)]Gal(β1-4)Gal(α1-}n.
26117825	7	1	theme	NPS	605:607	arg1	resonance					565:573	(13)C nuclear magnetic resonance	542:573	(13)C nuclear magnetic resonance	542:573	Sugar and methylation analyses, (1)H and (13)C nuclear magnetic resonance, and mass spectrometry of the NPS or of its specifically modified products allowed determining the repeating unit sequence: {2)Glc(α1-3)Glc(β1-3)[Gal(β1-4)]Gal(β1-4)Gal(α1-}n.
26117825	7	1	theme	NPS	605:607	arg1	H					536:536	(1)H	533:536	(1)H	533:536	Sugar and methylation analyses, (1)H and (13)C nuclear magnetic resonance, and mass spectrometry of the NPS or of its specifically modified products allowed determining the repeating unit sequence: {2)Glc(α1-3)Glc(β1-3)[Gal(β1-4)]Gal(β1-4)Gal(α1-}n.
26117825	7	1	theme	NPS	605:607	arg1	analyses					523:530	Sugar and methylation analyses	501:530	analyses	523:530	Sugar and methylation analyses, (1)H and (13)C nuclear magnetic resonance, and mass spectrometry of the NPS or of its specifically modified products allowed determining the repeating unit sequence: {2)Glc(α1-3)Glc(β1-3)[Gal(β1-4)]Gal(β1-4)Gal(α1-}n.
26117825	8	2	dep	that	780:783	arg1	is					765:766	is	765:766	is compared to that of exopolysaccharides produced by other Lactobacillus bulgaricus strains	765:856	The structure is compared to that of exopolysaccharides produced by other Lactobacillus bulgaricus strains.
26117825	2	3	theme	exopolysaccharide	137:153	arg1	NPS					156:158	The neutral exopolysaccharide (NPS)	125:159	The neutral exopolysaccharide (NPS) of Lactobacillus delbrueckii subsp.	125:195	The neutral exopolysaccharide (NPS) of Lactobacillus delbrueckii subsp.
26117825	3	4	theme	bulgaricus	197:206	arg1	OLL1073R-1					215:224	bulgaricus strain OLL1073R-1	197:224	bulgaricus strain OLL1073R-1	197:224	bulgaricus strain OLL1073R-1 was purified and characterized.
26117825	4	5	theme	molecular	262:270	arg1	 g/mol					290:295	5.0×10(6) g/mol	281:295	5.0×10(6) g/mol	281:295	The molecular mass was 5.0×10(6) g/mol.
26117825	4	5	theme	molecular	262:270	arg1	mass					272:275	The molecular mass	258:275	The molecular mass	258:275	The molecular mass was 5.0×10(6) g/mol.
26117825	7	6	theme	{	699:699	arg1	Gal					740:742	{2)Glc(α1-3)Glc(β1-3)[Gal(β1-4)]Gal(β1-4)Gal	699:742	the repeating unit sequence: {2)Glc(α1-3)Glc(β1-3)[Gal(β1-4)]Gal(β1-4)Gal(α1-}n	670:748	Sugar and methylation analyses, (1)H and (13)C nuclear magnetic resonance, and mass spectrometry of the NPS or of its specifically modified products allowed determining the repeating unit sequence: {2)Glc(α1-3)Glc(β1-3)[Gal(β1-4)]Gal(β1-4)Gal(α1-}n.
26117825	2	7	theme	neutral	129:135	arg1	NPS					156:158	The neutral exopolysaccharide (NPS)	125:159	The neutral exopolysaccharide (NPS) of Lactobacillus delbrueckii subsp.	125:195	The neutral exopolysaccharide (NPS) of Lactobacillus delbrueckii subsp.
26117825	3	8	theme	strain	208:213	arg1	OLL1073R-1					215:224	bulgaricus strain OLL1073R-1	197:224	bulgaricus strain OLL1073R-1	197:224	bulgaricus strain OLL1073R-1 was purified and characterized.
26117825	7	9	theme	α1-	744:746	arg1	n					748:748	α1-}n	744:748	the repeating unit sequence: {2)Glc(α1-3)Glc(β1-3)[Gal(β1-4)]Gal(β1-4)Gal(α1-}n	670:748	Sugar and methylation analyses, (1)H and (13)C nuclear magnetic resonance, and mass spectrometry of the NPS or of its specifically modified products allowed determining the repeating unit sequence: {2)Glc(α1-3)Glc(β1-3)[Gal(β1-4)]Gal(β1-4)Gal(α1-}n.
26117825	7	10	theme	unit	684:687	arg1	sequence					689:696	the repeating unit sequence	670:696	the repeating unit sequence: {2)Glc(α1-3)Glc(β1-3)[Gal(β1-4)]Gal(β1-4)Gal(α1-}n	670:748	Sugar and methylation analyses, (1)H and (13)C nuclear magnetic resonance, and mass spectrometry of the NPS or of its specifically modified products allowed determining the repeating unit sequence: {2)Glc(α1-3)Glc(β1-3)[Gal(β1-4)]Gal(β1-4)Gal(α1-}n.
26117825	2	11	theme	delbrueckii	178:188	arg1	subsp					190:194	Lactobacillus delbrueckii subsp	164:194	Lactobacillus delbrueckii subsp	164:194	The neutral exopolysaccharide (NPS) of Lactobacillus delbrueckii subsp.
26117825	6	12	theme	periodate	424:432	arg1	oxidation					434:442	periodate oxidation	424:442	periodate oxidation followed by borohydride reduction and Smith degradation	424:498	The NPS was also submitted to periodate oxidation followed by borohydride reduction and Smith degradation.
26117825	8	13	theme	bulgaricus	839:848	arg1	strains					850:856	other Lactobacillus bulgaricus strains	819:856	other Lactobacillus bulgaricus strains	819:856	The structure is compared to that of exopolysaccharides produced by other Lactobacillus bulgaricus strains.
26117825	2	14	theme	Lactobacillus	164:176	arg1	subsp					190:194	Lactobacillus delbrueckii subsp	164:194	Lactobacillus delbrueckii subsp	164:194	The neutral exopolysaccharide (NPS) of Lactobacillus delbrueckii subsp.
26117825	7	15	theme	nuclear	548:554	arg1	resonance					565:573	(13)C nuclear magnetic resonance	542:573	(13)C nuclear magnetic resonance	542:573	Sugar and methylation analyses, (1)H and (13)C nuclear magnetic resonance, and mass spectrometry of the NPS or of its specifically modified products allowed determining the repeating unit sequence: {2)Glc(α1-3)Glc(β1-3)[Gal(β1-4)]Gal(β1-4)Gal(α1-}n.
26117825	7	16	theme	Sugar	501:505	arg1	analyses					523:530	Sugar and methylation analyses	501:530	analyses	523:530	Sugar and methylation analyses, (1)H and (13)C nuclear magnetic resonance, and mass spectrometry of the NPS or of its specifically modified products allowed determining the repeating unit sequence: {2)Glc(α1-3)Glc(β1-3)[Gal(β1-4)]Gal(β1-4)Gal(α1-}n.
26117825	7	17	theme	magnetic	556:563	arg1	resonance					565:573	(13)C nuclear magnetic resonance	542:573	(13)C nuclear magnetic resonance	542:573	Sugar and methylation analyses, (1)H and (13)C nuclear magnetic resonance, and mass spectrometry of the NPS or of its specifically modified products allowed determining the repeating unit sequence: {2)Glc(α1-3)Glc(β1-3)[Gal(β1-4)]Gal(β1-4)Gal(α1-}n.
26117825	5	18	dep	gave	340:343	arg1	1					379:379	1	379:379	1	379:379	Sugar and absolute configuration analyses gave the following composition: d-Glc, 1; d-Gal, 1.5.
26117825	5	18	dep	gave	340:343	arg1	d-Glc					372:376	d-Glc	372:376	d-Glc	372:376	Sugar and absolute configuration analyses gave the following composition: d-Glc, 1; d-Gal, 1.5.
26117825	1	19	theme	bulgaricus	102:111	arg1	OLL1073R-1					113:122	bulgaricus OLL1073R-1	102:122	bulgaricus OLL1073R-1.	102:123	bulgaricus OLL1073R-1.
26117825	7	20	dep	H	536:536	arg1	1					534:534	1	534:534	1	534:534	Sugar and methylation analyses, (1)H and (13)C nuclear magnetic resonance, and mass spectrometry of the NPS or of its specifically modified products allowed determining the repeating unit sequence: {2)Glc(α1-3)Glc(β1-3)[Gal(β1-4)]Gal(β1-4)Gal(α1-}n.
26117825	0	21	theme	Structure	0:8	arg1	determination					10:22	Structure determination	0:22	Structure determination of the neutral exopolysaccharide	0:55	Structure determination of the neutral exopolysaccharide produced by Lactobacillus delbrueckii subsp.
26117825	7	22	theme	[Gal	720:723	arg1	Gal					740:742	{2)Glc(α1-3)Glc(β1-3)[Gal(β1-4)]Gal(β1-4)Gal	699:742	the repeating unit sequence: {2)Glc(α1-3)Glc(β1-3)[Gal(β1-4)]Gal(β1-4)Gal(α1-}n	670:748	Sugar and methylation analyses, (1)H and (13)C nuclear magnetic resonance, and mass spectrometry of the NPS or of its specifically modified products allowed determining the repeating unit sequence: {2)Glc(α1-3)Glc(β1-3)[Gal(β1-4)]Gal(β1-4)Gal(α1-}n.
26117825	7	23	theme	C	546:546	arg1	resonance					565:573	(13)C nuclear magnetic resonance	542:573	(13)C nuclear magnetic resonance	542:573	Sugar and methylation analyses, (1)H and (13)C nuclear magnetic resonance, and mass spectrometry of the NPS or of its specifically modified products allowed determining the repeating unit sequence: {2)Glc(α1-3)Glc(β1-3)[Gal(β1-4)]Gal(β1-4)Gal(α1-}n.
26117825	7	24	theme	Gal	731:733	arg1	Gal					740:742	{2)Glc(α1-3)Glc(β1-3)[Gal(β1-4)]Gal(β1-4)Gal	699:742	the repeating unit sequence: {2)Glc(α1-3)Glc(β1-3)[Gal(β1-4)]Gal(β1-4)Gal(α1-}n	670:748	Sugar and methylation analyses, (1)H and (13)C nuclear magnetic resonance, and mass spectrometry of the NPS or of its specifically modified products allowed determining the repeating unit sequence: {2)Glc(α1-3)Glc(β1-3)[Gal(β1-4)]Gal(β1-4)Gal(α1-}n.
26117825	5	25	theme	absolute	308:315	arg1	configuration					317:329	absolute configuration	308:329	absolute configuration	308:329	Sugar and absolute configuration analyses gave the following composition: d-Glc, 1; d-Gal, 1.5.
26117825	7	26	theme	β1-3	715:718	arg1	Gal					740:742	{2)Glc(α1-3)Glc(β1-3)[Gal(β1-4)]Gal(β1-4)Gal	699:742	the repeating unit sequence: {2)Glc(α1-3)Glc(β1-3)[Gal(β1-4)]Gal(β1-4)Gal(α1-}n	670:748	Sugar and methylation analyses, (1)H and (13)C nuclear magnetic resonance, and mass spectrometry of the NPS or of its specifically modified products allowed determining the repeating unit sequence: {2)Glc(α1-3)Glc(β1-3)[Gal(β1-4)]Gal(β1-4)Gal(α1-}n.
26117825	7	27	theme	β1-4	725:728	arg1	Gal					740:742	{2)Glc(α1-3)Glc(β1-3)[Gal(β1-4)]Gal(β1-4)Gal	699:742	the repeating unit sequence: {2)Glc(α1-3)Glc(β1-3)[Gal(β1-4)]Gal(β1-4)Gal(α1-}n	670:748	Sugar and methylation analyses, (1)H and (13)C nuclear magnetic resonance, and mass spectrometry of the NPS or of its specifically modified products allowed determining the repeating unit sequence: {2)Glc(α1-3)Glc(β1-3)[Gal(β1-4)]Gal(β1-4)Gal(α1-}n.
26117825	0	28	theme	exopolysaccharide	39:55	arg1	determination					10:22	Structure determination	0:22	Structure determination of the neutral exopolysaccharide	0:55	Structure determination of the neutral exopolysaccharide produced by Lactobacillus delbrueckii subsp.
26117825	7	29	theme	repeating	674:682	arg1	sequence					689:696	the repeating unit sequence	670:696	the repeating unit sequence: {2)Glc(α1-3)Glc(β1-3)[Gal(β1-4)]Gal(β1-4)Gal(α1-}n	670:748	Sugar and methylation analyses, (1)H and (13)C nuclear magnetic resonance, and mass spectrometry of the NPS or of its specifically modified products allowed determining the repeating unit sequence: {2)Glc(α1-3)Glc(β1-3)[Gal(β1-4)]Gal(β1-4)Gal(α1-}n.
26117825	0	30	theme	neutral	31:37	arg1	exopolysaccharide					39:55	the neutral exopolysaccharide	27:55	the neutral exopolysaccharide	27:55	Structure determination of the neutral exopolysaccharide produced by Lactobacillus delbrueckii subsp.
26117825	7	31	theme	Glc	711:713	arg1	Gal					740:742	{2)Glc(α1-3)Glc(β1-3)[Gal(β1-4)]Gal(β1-4)Gal	699:742	the repeating unit sequence: {2)Glc(α1-3)Glc(β1-3)[Gal(β1-4)]Gal(β1-4)Gal(α1-}n	670:748	Sugar and methylation analyses, (1)H and (13)C nuclear magnetic resonance, and mass spectrometry of the NPS or of its specifically modified products allowed determining the repeating unit sequence: {2)Glc(α1-3)Glc(β1-3)[Gal(β1-4)]Gal(β1-4)Gal(α1-}n.
26117825	6	32	theme	Smith	482:486	arg1	degradation					488:498	Smith degradation	482:498	Smith degradation	482:498	The NPS was also submitted to periodate oxidation followed by borohydride reduction and Smith degradation.
26117825	2	33	theme	subsp	190:194	arg1	NPS					156:158	The neutral exopolysaccharide (NPS)	125:159	The neutral exopolysaccharide (NPS) of Lactobacillus delbrueckii subsp.	125:195	The neutral exopolysaccharide (NPS) of Lactobacillus delbrueckii subsp.
26117825	5	34	theme	configuration	317:329	arg1	analyses					331:338	Sugar and absolute configuration analyses	298:338	Sugar and absolute configuration analyses	298:338	Sugar and absolute configuration analyses gave the following composition: d-Glc, 1; d-Gal, 1.5.
26117825	7	35	theme	α1-3	706:709	arg1	Gal					740:742	{2)Glc(α1-3)Glc(β1-3)[Gal(β1-4)]Gal(β1-4)Gal	699:742	the repeating unit sequence: {2)Glc(α1-3)Glc(β1-3)[Gal(β1-4)]Gal(β1-4)Gal(α1-}n	670:748	Sugar and methylation analyses, (1)H and (13)C nuclear magnetic resonance, and mass spectrometry of the NPS or of its specifically modified products allowed determining the repeating unit sequence: {2)Glc(α1-3)Glc(β1-3)[Gal(β1-4)]Gal(β1-4)Gal(α1-}n.
26117825	7	36	theme	modified	632:639	arg1	products					641:648	its specifically modified products	615:648	its specifically modified products	615:648	Sugar and methylation analyses, (1)H and (13)C nuclear magnetic resonance, and mass spectrometry of the NPS or of its specifically modified products allowed determining the repeating unit sequence: {2)Glc(α1-3)Glc(β1-3)[Gal(β1-4)]Gal(β1-4)Gal(α1-}n.
26117825	7	37	dep	sequence	689:696	arg1	n					748:748	α1-}n	744:748	the repeating unit sequence: {2)Glc(α1-3)Glc(β1-3)[Gal(β1-4)]Gal(β1-4)Gal(α1-}n	670:748	Sugar and methylation analyses, (1)H and (13)C nuclear magnetic resonance, and mass spectrometry of the NPS or of its specifically modified products allowed determining the repeating unit sequence: {2)Glc(α1-3)Glc(β1-3)[Gal(β1-4)]Gal(β1-4)Gal(α1-}n.
26117825	7	37	dep	sequence	689:696	arg1	Gal					740:742	{2)Glc(α1-3)Glc(β1-3)[Gal(β1-4)]Gal(β1-4)Gal	699:742	the repeating unit sequence: {2)Glc(α1-3)Glc(β1-3)[Gal(β1-4)]Gal(β1-4)Gal(α1-}n	670:748	Sugar and methylation analyses, (1)H and (13)C nuclear magnetic resonance, and mass spectrometry of the NPS or of its specifically modified products allowed determining the repeating unit sequence: {2)Glc(α1-3)Glc(β1-3)[Gal(β1-4)]Gal(β1-4)Gal(α1-}n.
26117825	5	38	theme	following	349:357	arg1	composition					359:369	the following composition	345:369	the following composition	345:369	Sugar and absolute configuration analyses gave the following composition: d-Glc, 1; d-Gal, 1.5.
26117825	7	39	theme	products	641:648	arg1	spectrometry					585:596	mass spectrometry	580:596	mass spectrometry	580:596	Sugar and methylation analyses, (1)H and (13)C nuclear magnetic resonance, and mass spectrometry of the NPS or of its specifically modified products allowed determining the repeating unit sequence: {2)Glc(α1-3)Glc(β1-3)[Gal(β1-4)]Gal(β1-4)Gal(α1-}n.
26117825	7	39	theme	products	641:648	arg1	resonance					565:573	(13)C nuclear magnetic resonance	542:573	(13)C nuclear magnetic resonance	542:573	Sugar and methylation analyses, (1)H and (13)C nuclear magnetic resonance, and mass spectrometry of the NPS or of its specifically modified products allowed determining the repeating unit sequence: {2)Glc(α1-3)Glc(β1-3)[Gal(β1-4)]Gal(β1-4)Gal(α1-}n.
26117825	7	39	theme	products	641:648	arg1	H					536:536	(1)H	533:536	(1)H	533:536	Sugar and methylation analyses, (1)H and (13)C nuclear magnetic resonance, and mass spectrometry of the NPS or of its specifically modified products allowed determining the repeating unit sequence: {2)Glc(α1-3)Glc(β1-3)[Gal(β1-4)]Gal(β1-4)Gal(α1-}n.
26117825	7	39	theme	products	641:648	arg1	analyses					523:530	Sugar and methylation analyses	501:530	analyses	523:530	Sugar and methylation analyses, (1)H and (13)C nuclear magnetic resonance, and mass spectrometry of the NPS or of its specifically modified products allowed determining the repeating unit sequence: {2)Glc(α1-3)Glc(β1-3)[Gal(β1-4)]Gal(β1-4)Gal(α1-}n.
26117825	7	40	theme	Glc	702:704	arg1	Gal					740:742	{2)Glc(α1-3)Glc(β1-3)[Gal(β1-4)]Gal(β1-4)Gal	699:742	the repeating unit sequence: {2)Glc(α1-3)Glc(β1-3)[Gal(β1-4)]Gal(β1-4)Gal(α1-}n	670:748	Sugar and methylation analyses, (1)H and (13)C nuclear magnetic resonance, and mass spectrometry of the NPS or of its specifically modified products allowed determining the repeating unit sequence: {2)Glc(α1-3)Glc(β1-3)[Gal(β1-4)]Gal(β1-4)Gal(α1-}n.
26117825	6	41	theme	borohydride	456:466	arg1	reduction					468:476	borohydride reduction	456:476	borohydride reduction	456:476	The NPS was also submitted to periodate oxidation followed by borohydride reduction and Smith degradation.
26117825	5	42	dep	1	379:379	arg1	d-Gal					382:386	d-Gal	382:386	d-Gal	382:386	Sugar and absolute configuration analyses gave the following composition: d-Glc, 1; d-Gal, 1.5.
26117825	0	43	theme	delbrueckii	83:93	arg1	subsp					95:99	Lactobacillus delbrueckii subsp	69:99	Lactobacillus delbrueckii subsp	69:99	Structure determination of the neutral exopolysaccharide produced by Lactobacillus delbrueckii subsp.
26117825	7	44	theme	methylation	511:521	arg1	analyses					523:530	Sugar and methylation analyses	501:530	analyses	523:530	Sugar and methylation analyses, (1)H and (13)C nuclear magnetic resonance, and mass spectrometry of the NPS or of its specifically modified products allowed determining the repeating unit sequence: {2)Glc(α1-3)Glc(β1-3)[Gal(β1-4)]Gal(β1-4)Gal(α1-}n.
26117825	0	45	theme	Lactobacillus	69:81	arg1	subsp					95:99	Lactobacillus delbrueckii subsp	69:99	Lactobacillus delbrueckii subsp	69:99	Structure determination of the neutral exopolysaccharide produced by Lactobacillus delbrueckii subsp.
26117825	5	46	theme	Sugar	298:302	arg1	analyses					331:338	Sugar and absolute configuration analyses	298:338	Sugar and absolute configuration analyses	298:338	Sugar and absolute configuration analyses gave the following composition: d-Glc, 1; d-Gal, 1.5.
26117825	8	47	theme	other	819:823	arg1	strains					850:856	other Lactobacillus bulgaricus strains	819:856	other Lactobacillus bulgaricus strains	819:856	The structure is compared to that of exopolysaccharides produced by other Lactobacillus bulgaricus strains.
26117825	7	48	theme	}	747:747	arg1	n					748:748	α1-}n	744:748	the repeating unit sequence: {2)Glc(α1-3)Glc(β1-3)[Gal(β1-4)]Gal(β1-4)Gal(α1-}n	670:748	Sugar and methylation analyses, (1)H and (13)C nuclear magnetic resonance, and mass spectrometry of the NPS or of its specifically modified products allowed determining the repeating unit sequence: {2)Glc(α1-3)Glc(β1-3)[Gal(β1-4)]Gal(β1-4)Gal(α1-}n.
28668611	3	0	with	water	526:530	arg1	ethanol					558:564	ethanol	558:564	ethanol	558:564	Performing different recovery methods, such as re-dissolving in water and re-precipitation with ethanol on produced EPS, provided schizophyllan with purity similar to the commercial grade.
28668611	1	1	theme	different	159:167	arg1	procedures					178:187	different recovery procedures	159:187	different recovery procedures	159:187	This study investigates the effects of different recovery procedures on high molar mass schizophyllan produced by Schizophyllum commune using low value agricultural residues.
28668611	0	2	theme	schizophyllan	80:92	arg1	properties					66:75	structural and rheological properties	39:75	structural and rheological properties of schizophyllan	39:92	Implications of recovery procedures on structural and rheological properties of schizophyllan produced from date syrup.
28668611	1	3	theme	recovery	169:176	arg1	procedures					178:187	different recovery procedures	159:187	different recovery procedures	159:187	This study investigates the effects of different recovery procedures on high molar mass schizophyllan produced by Schizophyllum commune using low value agricultural residues.
28668611	1	4	theme	agricultural	272:283	arg1	residues					285:292	low value agricultural residues	262:292	low value agricultural residues	262:292	This study investigates the effects of different recovery procedures on high molar mass schizophyllan produced by Schizophyllum commune using low value agricultural residues.
28668611	0	5	from	Implications	0:11	arg1	properties					66:75	structural and rheological properties	39:75	structural and rheological properties of schizophyllan	39:92	Implications of recovery procedures on structural and rheological properties of schizophyllan produced from date syrup.
28668611	3	6	with	re-precipitation	536:551	arg1	ethanol					558:564	ethanol	558:564	ethanol	558:564	Performing different recovery methods, such as re-dissolving in water and re-precipitation with ethanol on produced EPS, provided schizophyllan with purity similar to the commercial grade.
28668611	3	7	theme	produced	569:576	arg1	EPS					578:580	produced EPS	569:580	produced EPS	569:580	Performing different recovery methods, such as re-dissolving in water and re-precipitation with ethanol on produced EPS, provided schizophyllan with purity similar to the commercial grade.
28668611	6	8	theme	excellent	944:952	arg1	properties					966:975	excellent rheological properties	944:975	excellent rheological properties using low value agricultural side products	944:1018	This study evidences the possibility of producing EPSs with excellent rheological properties using low value agricultural side products.
28668611	4	9	dep	branching	734:742	arg1	degree					744:749	degree	744:749	degree	744:749	Besides, Freeze-thawing cycles allowed the fractionation of schizophyllan based on branching degree and solubility.
28668611	1	10	from	effects	148:154	arg1	schizophyllan					208:220	high molar mass schizophyllan	192:220	high molar mass schizophyllan produced by Schizophyllum commune using low value agricultural residues	192:292	This study investigates the effects of different recovery procedures on high molar mass schizophyllan produced by Schizophyllum commune using low value agricultural residues.
28668611	2	11	theme	sugar	376:380	arg1	composition					382:392	sugar composition	376:392	sugar composition	376:392	Recovered extracellular polysaccharides (EPSs) were compared in terms of purity, sugar composition, degree of branching, molecular weight, and rheological properties.
28668611	5	12	theme	higher	781:786	arg1	purity					788:793	higher purity	781:793	higher purity	781:793	The EPSs with higher purity and lower degree of branching (less conformational flexibility) showed higher viscosity.
28668611	2	13	theme	extracellular	305:317	arg1	polysaccharides					319:333	extracellular polysaccharides	305:333	Recovered extracellular polysaccharides (EPSs)	295:340	Recovered extracellular polysaccharides (EPSs) were compared in terms of purity, sugar composition, degree of branching, molecular weight, and rheological properties.
28668611	2	13	theme	extracellular	305:317	arg1	EPSs					336:339	EPSs	336:339	EPSs	336:339	Recovered extracellular polysaccharides (EPSs) were compared in terms of purity, sugar composition, degree of branching, molecular weight, and rheological properties.
28668611	4	14	theme	schizophyllan	711:723	arg1	fractionation					694:706	the fractionation	690:706	the fractionation of schizophyllan based on branching degree and solubility	690:764	Besides, Freeze-thawing cycles allowed the fractionation of schizophyllan based on branching degree and solubility.
28668611	1	15	theme	procedures	178:187	arg1	effects					148:154	the effects	144:154	the effects of different recovery procedures on high molar mass schizophyllan produced by Schizophyllum commune using low value agricultural residues	144:292	This study investigates the effects of different recovery procedures on high molar mass schizophyllan produced by Schizophyllum commune using low value agricultural residues.
28668611	6	16	theme	EPSs	934:937	arg1	possibility					909:919	the possibility	905:919	the possibility of producing EPSs with excellent rheological properties using low value agricultural side products	905:1018	This study evidences the possibility of producing EPSs with excellent rheological properties using low value agricultural side products.
28668611	5	17	theme	branching	815:823	arg1	EPSs					771:774	The EPSs	767:774	The EPSs with higher purity	767:793	The EPSs with higher purity and lower degree of branching (less conformational flexibility) showed higher viscosity.
28668611	5	17	theme	branching	815:823	arg1	degree					805:810	lower degree	799:810	lower degree of branching (less conformational flexibility)	799:857	The EPSs with higher purity and lower degree of branching (less conformational flexibility) showed higher viscosity.
28668611	2	18	theme	Recovered	295:303	arg1	polysaccharides					319:333	extracellular polysaccharides	305:333	Recovered extracellular polysaccharides (EPSs)	295:340	Recovered extracellular polysaccharides (EPSs) were compared in terms of purity, sugar composition, degree of branching, molecular weight, and rheological properties.
28668611	2	18	theme	Recovered	295:303	arg1	EPSs					336:339	EPSs	336:339	EPSs	336:339	Recovered extracellular polysaccharides (EPSs) were compared in terms of purity, sugar composition, degree of branching, molecular weight, and rheological properties.
28668611	2	19	theme	branching	405:413	arg1	weight					426:431	molecular weight	416:431	molecular weight	416:431	Recovered extracellular polysaccharides (EPSs) were compared in terms of purity, sugar composition, degree of branching, molecular weight, and rheological properties.
28668611	2	19	theme	branching	405:413	arg1	degree					395:400	degree	395:400	degree of branching	395:413	Recovered extracellular polysaccharides (EPSs) were compared in terms of purity, sugar composition, degree of branching, molecular weight, and rheological properties.
28668611	2	19	theme	branching	405:413	arg1	purity					368:373	purity	368:373	purity	368:373	Recovered extracellular polysaccharides (EPSs) were compared in terms of purity, sugar composition, degree of branching, molecular weight, and rheological properties.
28668611	2	19	theme	branching	405:413	arg1	composition					382:392	sugar composition	376:392	sugar composition	376:392	Recovered extracellular polysaccharides (EPSs) were compared in terms of purity, sugar composition, degree of branching, molecular weight, and rheological properties.
28668611	2	19	theme	branching	405:413	arg1	properties					450:459	rheological properties	438:459	rheological properties	438:459	Recovered extracellular polysaccharides (EPSs) were compared in terms of purity, sugar composition, degree of branching, molecular weight, and rheological properties.
28668611	6	20	theme	producing	924:932	arg1	EPSs					934:937	producing EPSs	924:937	producing EPSs with excellent rheological properties using low value agricultural side products	924:1018	This study evidences the possibility of producing EPSs with excellent rheological properties using low value agricultural side products.
28668611	1	21	theme	high	192:195	arg1	schizophyllan					208:220	high molar mass schizophyllan	192:220	high molar mass schizophyllan produced by Schizophyllum commune using low value agricultural residues	192:292	This study investigates the effects of different recovery procedures on high molar mass schizophyllan produced by Schizophyllum commune using low value agricultural residues.
28668611	6	22	theme	value	987:991	arg1	products					1011:1018	low value agricultural side products	983:1018	low value agricultural side products	983:1018	This study evidences the possibility of producing EPSs with excellent rheological properties using low value agricultural side products.
28668611	3	23	from	re-dissolving	509:521	arg1	EPS					578:580	produced EPS	569:580	produced EPS	569:580	Performing different recovery methods, such as re-dissolving in water and re-precipitation with ethanol on produced EPS, provided schizophyllan with purity similar to the commercial grade.
28668611	3	23	from	re-dissolving	509:521	arg1	re-precipitation					536:551	re-precipitation	536:551	re-precipitation	536:551	Performing different recovery methods, such as re-dissolving in water and re-precipitation with ethanol on produced EPS, provided schizophyllan with purity similar to the commercial grade.
28668611	3	23	from	re-dissolving	509:521	arg1	water					526:530	water	526:530	water	526:530	Performing different recovery methods, such as re-dissolving in water and re-precipitation with ethanol on produced EPS, provided schizophyllan with purity similar to the commercial grade.
28668611	2	24	theme	degree	395:400	arg1	terms					359:363	terms	359:363	terms of purity, sugar composition, degree of branching, molecular weight, and rheological properties	359:459	Recovered extracellular polysaccharides (EPSs) were compared in terms of purity, sugar composition, degree of branching, molecular weight, and rheological properties.
28668611	0	25	theme	procedures	25:34	arg1	Implications					0:11	Implications	0:11	Implications of recovery procedures on structural and rheological properties of schizophyllan	0:92	Implications of recovery procedures on structural and rheological properties of schizophyllan produced from date syrup.
28668611	7	26	theme	specific	1202:1209	arg1	applications					1211:1222	specific applications	1202:1222	specific applications	1202:1222	Furthermore, our results demonstrate the importance of recovery methods for tailoring the purity, molecular structure and macroscopic properties of the produced polysaccharides for specific applications.
28668611	7	27	theme	produced	1173:1180	arg1	polysaccharides					1182:1196	the produced polysaccharides	1169:1196	the produced polysaccharides	1169:1196	Furthermore, our results demonstrate the importance of recovery methods for tailoring the purity, molecular structure and macroscopic properties of the produced polysaccharides for specific applications.
28668611	1	28	theme	mass	203:206	arg1	schizophyllan					208:220	high molar mass schizophyllan	192:220	high molar mass schizophyllan produced by Schizophyllum commune using low value agricultural residues	192:292	This study investigates the effects of different recovery procedures on high molar mass schizophyllan produced by Schizophyllum commune using low value agricultural residues.
28668611	0	29	theme	recovery	16:23	arg1	procedures					25:34	recovery procedures	16:34	recovery procedures	16:34	Implications of recovery procedures on structural and rheological properties of schizophyllan produced from date syrup.
28668611	7	30	theme	macroscopic	1143:1153	arg1	properties					1155:1164	macroscopic properties	1143:1164	macroscopic properties	1143:1164	Furthermore, our results demonstrate the importance of recovery methods for tailoring the purity, molecular structure and macroscopic properties of the produced polysaccharides for specific applications.
28668611	0	31	theme	structural	39:48	arg1	properties					66:75	structural and rheological properties	39:75	structural and rheological properties of schizophyllan	39:92	Implications of recovery procedures on structural and rheological properties of schizophyllan produced from date syrup.
28668611	7	32	theme	molecular	1119:1127	arg1	structure					1129:1137	molecular structure	1119:1137	molecular structure	1119:1137	Furthermore, our results demonstrate the importance of recovery methods for tailoring the purity, molecular structure and macroscopic properties of the produced polysaccharides for specific applications.
28668611	3	33	theme	different	473:481	arg1	re-dissolving					509:521	re-dissolving	509:521	re-dissolving in water and re-precipitation with ethanol on produced EPS	509:580	Performing different recovery methods, such as re-dissolving in water and re-precipitation with ethanol on produced EPS, provided schizophyllan with purity similar to the commercial grade.
28668611	3	33	theme	different	473:481	arg1	methods					492:498	Performing different recovery methods	462:498	Performing different recovery methods	462:498	Performing different recovery methods, such as re-dissolving in water and re-precipitation with ethanol on produced EPS, provided schizophyllan with purity similar to the commercial grade.
28668611	1	34	theme	molar	197:201	arg1	schizophyllan					208:220	high molar mass schizophyllan	192:220	high molar mass schizophyllan produced by Schizophyllum commune using low value agricultural residues	192:292	This study investigates the effects of different recovery procedures on high molar mass schizophyllan produced by Schizophyllum commune using low value agricultural residues.
28668611	2	35	theme	weight	426:431	arg1	terms					359:363	terms	359:363	terms of purity, sugar composition, degree of branching, molecular weight, and rheological properties	359:459	Recovered extracellular polysaccharides (EPSs) were compared in terms of purity, sugar composition, degree of branching, molecular weight, and rheological properties.
28668611	2	36	theme	properties	450:459	arg1	terms					359:363	terms	359:363	terms of purity, sugar composition, degree of branching, molecular weight, and rheological properties	359:459	Recovered extracellular polysaccharides (EPSs) were compared in terms of purity, sugar composition, degree of branching, molecular weight, and rheological properties.
28668611	7	37	theme	methods	1085:1091	arg1	importance					1062:1071	the importance	1058:1071	the importance of recovery methods for tailoring the purity, molecular structure and macroscopic properties of the produced polysaccharides for specific applications	1058:1222	Furthermore, our results demonstrate the importance of recovery methods for tailoring the purity, molecular structure and macroscopic properties of the produced polysaccharides for specific applications.
28668611	2	38	theme	molecular	416:424	arg1	weight					426:431	molecular weight	416:431	molecular weight	416:431	Recovered extracellular polysaccharides (EPSs) were compared in terms of purity, sugar composition, degree of branching, molecular weight, and rheological properties.
28668611	0	39	theme	rheological	54:64	arg1	properties					66:75	structural and rheological properties	39:75	structural and rheological properties of schizophyllan	39:92	Implications of recovery procedures on structural and rheological properties of schizophyllan produced from date syrup.
28668611	6	40	theme	side	1006:1009	arg1	products					1011:1018	low value agricultural side products	983:1018	low value agricultural side products	983:1018	This study evidences the possibility of producing EPSs with excellent rheological properties using low value agricultural side products.
28668611	7	41	dep	purity	1111:1116	arg1	the					1107:1109	the	1107:1109	the	1107:1109	Furthermore, our results demonstrate the importance of recovery methods for tailoring the purity, molecular structure and macroscopic properties of the produced polysaccharides for specific applications.
28668611	5	42	with	EPSs	771:774	arg1	purity					788:793	higher purity	781:793	higher purity	781:793	The EPSs with higher purity and lower degree of branching (less conformational flexibility) showed higher viscosity.
28668611	2	43	theme	purity	368:373	arg1	terms					359:363	terms	359:363	terms of purity, sugar composition, degree of branching, molecular weight, and rheological properties	359:459	Recovered extracellular polysaccharides (EPSs) were compared in terms of purity, sugar composition, degree of branching, molecular weight, and rheological properties.
28668611	5	44	theme	conformational	831:844	arg1	branching					815:823	branching	815:823	branching (less conformational flexibility)	815:857	The EPSs with higher purity and lower degree of branching (less conformational flexibility) showed higher viscosity.
28668611	5	44	theme	conformational	831:844	arg1	flexibility					846:856	less conformational flexibility	826:856	less conformational flexibility	826:856	The EPSs with higher purity and lower degree of branching (less conformational flexibility) showed higher viscosity.
28668611	6	45	theme	agricultural	993:1004	arg1	products					1011:1018	low value agricultural side products	983:1018	low value agricultural side products	983:1018	This study evidences the possibility of producing EPSs with excellent rheological properties using low value agricultural side products.
28668611	3	46	theme	similar	618:624	arg1	schizophyllan					592:604	schizophyllan	592:604	schizophyllan with purity similar to the commercial grade	592:648	Performing different recovery methods, such as re-dissolving in water and re-precipitation with ethanol on produced EPS, provided schizophyllan with purity similar to the commercial grade.
28668611	7	47	theme	polysaccharides	1182:1196	arg1	purity					1111:1116	purity	1111:1116	purity	1111:1116	Furthermore, our results demonstrate the importance of recovery methods for tailoring the purity, molecular structure and macroscopic properties of the produced polysaccharides for specific applications.
28668611	7	47	theme	polysaccharides	1182:1196	arg1	structure					1129:1137	molecular structure	1119:1137	molecular structure	1119:1137	Furthermore, our results demonstrate the importance of recovery methods for tailoring the purity, molecular structure and macroscopic properties of the produced polysaccharides for specific applications.
28668611	7	47	theme	polysaccharides	1182:1196	arg1	properties					1155:1164	macroscopic properties	1143:1164	macroscopic properties	1143:1164	Furthermore, our results demonstrate the importance of recovery methods for tailoring the purity, molecular structure and macroscopic properties of the produced polysaccharides for specific applications.
28668611	3	48	with	schizophyllan	592:604	arg1	purity					611:616	purity	611:616	purity	611:616	Performing different recovery methods, such as re-dissolving in water and re-precipitation with ethanol on produced EPS, provided schizophyllan with purity similar to the commercial grade.
28668611	5	49	with	degree	805:810	arg1	purity					788:793	higher purity	781:793	higher purity	781:793	The EPSs with higher purity and lower degree of branching (less conformational flexibility) showed higher viscosity.
28668611	7	50	theme	recovery	1076:1083	arg1	methods					1085:1091	recovery methods	1076:1091	recovery methods	1076:1091	Furthermore, our results demonstrate the importance of recovery methods for tailoring the purity, molecular structure and macroscopic properties of the produced polysaccharides for specific applications.
28668611	1	51	theme	Schizophyllum	234:246	arg1	commune					248:254	Schizophyllum commune	234:254	Schizophyllum commune using low value agricultural residues	234:292	This study investigates the effects of different recovery procedures on high molar mass schizophyllan produced by Schizophyllum commune using low value agricultural residues.
28668611	5	52	theme	higher	866:871	arg1	viscosity					873:881	higher viscosity	866:881	higher viscosity	866:881	The EPSs with higher purity and lower degree of branching (less conformational flexibility) showed higher viscosity.
28668611	3	53	theme	recovery	483:490	arg1	re-dissolving					509:521	re-dissolving	509:521	re-dissolving in water and re-precipitation with ethanol on produced EPS	509:580	Performing different recovery methods, such as re-dissolving in water and re-precipitation with ethanol on produced EPS, provided schizophyllan with purity similar to the commercial grade.
28668611	3	53	theme	recovery	483:490	arg1	methods					492:498	Performing different recovery methods	462:498	Performing different recovery methods	462:498	Performing different recovery methods, such as re-dissolving in water and re-precipitation with ethanol on produced EPS, provided schizophyllan with purity similar to the commercial grade.
28668611	3	54	theme	commercial	633:642	arg1	grade					644:648	the commercial grade	629:648	the commercial grade	629:648	Performing different recovery methods, such as re-dissolving in water and re-precipitation with ethanol on produced EPS, provided schizophyllan with purity similar to the commercial grade.
28668611	4	55	theme	Freeze-thawing	660:673	arg1	cycles					675:680	Freeze-thawing cycles	660:680	Freeze-thawing cycles	660:680	Besides, Freeze-thawing cycles allowed the fractionation of schizophyllan based on branching degree and solubility.
28668611	6	56	with	EPSs	934:937	arg1	properties					966:975	excellent rheological properties	944:975	excellent rheological properties using low value agricultural side products	944:1018	This study evidences the possibility of producing EPSs with excellent rheological properties using low value agricultural side products.
28668611	3	57	theme	Performing	462:471	arg1	re-dissolving					509:521	re-dissolving	509:521	re-dissolving in water and re-precipitation with ethanol on produced EPS	509:580	Performing different recovery methods, such as re-dissolving in water and re-precipitation with ethanol on produced EPS, provided schizophyllan with purity similar to the commercial grade.
28668611	3	57	theme	Performing	462:471	arg1	methods					492:498	Performing different recovery methods	462:498	Performing different recovery methods	462:498	Performing different recovery methods, such as re-dissolving in water and re-precipitation with ethanol on produced EPS, provided schizophyllan with purity similar to the commercial grade.
28668611	6	58	theme	rheological	954:964	arg1	properties					966:975	excellent rheological properties	944:975	excellent rheological properties using low value agricultural side products	944:1018	This study evidences the possibility of producing EPSs with excellent rheological properties using low value agricultural side products.
28668611	5	59	theme	lower	799:803	arg1	degree					805:810	lower degree	799:810	lower degree of branching (less conformational flexibility)	799:857	The EPSs with higher purity and lower degree of branching (less conformational flexibility) showed higher viscosity.
28668611	6	60	theme	low	983:985	arg1	products					1011:1018	low value agricultural side products	983:1018	low value agricultural side products	983:1018	This study evidences the possibility of producing EPSs with excellent rheological properties using low value agricultural side products.
28668611	2	61	theme	rheological	438:448	arg1	properties					450:459	rheological properties	438:459	rheological properties	438:459	Recovered extracellular polysaccharides (EPSs) were compared in terms of purity, sugar composition, degree of branching, molecular weight, and rheological properties.
28668611	2	62	theme	composition	382:392	arg1	terms					359:363	terms	359:363	terms of purity, sugar composition, degree of branching, molecular weight, and rheological properties	359:459	Recovered extracellular polysaccharides (EPSs) were compared in terms of purity, sugar composition, degree of branching, molecular weight, and rheological properties.
26572415	3	0	theme	average	263:269	arg1	Mw					289:290	Mw	289:290	Mw	289:290	The average molecular weight (Mw) of EUPS was 11.4632 × 10(5)Da.
26572415	3	0	theme	average	263:269	arg1	Da					320:321	11.4632 × 10(5)Da	305:321	11.4632 × 10(5)Da	305:321	The average molecular weight (Mw) of EUPS was 11.4632 × 10(5)Da.
26572415	3	0	theme	average	263:269	arg1	weight					281:286	The average molecular weight	259:286	The average molecular weight (Mw) of EUPS	259:299	The average molecular weight (Mw) of EUPS was 11.4632 × 10(5)Da.
26572415	4	1	theme	%	491:491	arg1	mass					443:446	a relative mass	432:446	a relative mass of 36.6%, 16.6%, 14.2%, 15.7%, 9.5% and 7.4%, respectively	432:505	The monosaccharide components of EUPS were glucose, fructose, mannose, fucose, galactose and arabinose with a relative mass of 36.6%, 16.6%, 14.2%, 15.7%, 9.5% and 7.4%, respectively.
26572415	3	2	theme	molecular	271:279	arg1	Mw					289:290	Mw	289:290	Mw	289:290	The average molecular weight (Mw) of EUPS was 11.4632 × 10(5)Da.
26572415	3	2	theme	molecular	271:279	arg1	Da					320:321	11.4632 × 10(5)Da	305:321	11.4632 × 10(5)Da	305:321	The average molecular weight (Mw) of EUPS was 11.4632 × 10(5)Da.
26572415	3	2	theme	molecular	271:279	arg1	weight					281:286	The average molecular weight	259:286	The average molecular weight (Mw) of EUPS	259:299	The average molecular weight (Mw) of EUPS was 11.4632 × 10(5)Da.
26572415	8	3	theme	strong	1052:1057	arg1	EUPS					1042:1045	EUPS	1042:1045	EUPS	1042:1045	Together, these results suggest that EUPS is a strong immunostimulant.
26572415	8	3	theme	strong	1052:1057	arg1	immunostimulant					1059:1073	a strong immunostimulant	1050:1073	a strong immunostimulant	1050:1073	Together, these results suggest that EUPS is a strong immunostimulant.
26572415	7	4	theme	antibody	963:970	arg1	IgG					935:937	the FMDV-specific IgG	917:937	the FMDV-specific IgG	917:937	In in vivo experiments, our data showed that EUPS could significantly enhance the FMDV-specific IgG, IgG1, IgG2a, and IgG2b antibody titers and T cell proliferation.
26572415	7	4	theme	antibody	963:970	arg1	titers					972:977	IgG1, IgG2a, and IgG2b antibody titers	940:977	IgG1, IgG2a, and IgG2b antibody titers	940:977	In in vivo experiments, our data showed that EUPS could significantly enhance the FMDV-specific IgG, IgG1, IgG2a, and IgG2b antibody titers and T cell proliferation.
26572415	5	5	theme	surface	603:609	arg1	expression					611:620	their surface expression	597:620	their surface expression of MHC I/II, CD80, CD40, and CD86	597:654	In in vitro experiments, EUPS (1.2-75 μg/mL) treatment of dendritic cells (DC) increased their surface expression of MHC I/II, CD80, CD40, and CD86 and indicated that EUPS induced DC maturation.
26572415	6	6	dep	cytokine	801:808	arg1	IFN-γ					820:824	IFN-γ	820:824	IFN-γ	820:824	Furthermore, EUPS also significantly enhanced lymphocyte proliferation and significantly enhanced cytokine (IL-4 and IFN-γ) production.
26572415	6	6	dep	cytokine	801:808	arg1	IL-4					811:814	IL-4	811:814	IL-4	811:814	Furthermore, EUPS also significantly enhanced lymphocyte proliferation and significantly enhanced cytokine (IL-4 and IFN-γ) production.
26572415	7	7	theme	IgG2b	957:961	arg1	IgG					935:937	the FMDV-specific IgG	917:937	the FMDV-specific IgG	917:937	In in vivo experiments, our data showed that EUPS could significantly enhance the FMDV-specific IgG, IgG1, IgG2a, and IgG2b antibody titers and T cell proliferation.
26572415	7	7	theme	IgG2b	957:961	arg1	titers					972:977	IgG1, IgG2a, and IgG2b antibody titers	940:977	IgG1, IgG2a, and IgG2b antibody titers	940:977	In in vivo experiments, our data showed that EUPS could significantly enhance the FMDV-specific IgG, IgG1, IgG2a, and IgG2b antibody titers and T cell proliferation.
26572415	5	8	theme	in	511:512	arg1	experiments					520:530	in vitro experiments	511:530	in vitro experiments	511:530	In in vitro experiments, EUPS (1.2-75 μg/mL) treatment of dendritic cells (DC) increased their surface expression of MHC I/II, CD80, CD40, and CD86 and indicated that EUPS induced DC maturation.
26572415	5	9	theme	cells	576:580	arg1	treatment					553:561	EUPS (1.2-75 μg/mL) treatment	533:561	EUPS (1.2-75 μg/mL) treatment of dendritic cells (DC)	533:585	In in vitro experiments, EUPS (1.2-75 μg/mL) treatment of dendritic cells (DC) increased their surface expression of MHC I/II, CD80, CD40, and CD86 and indicated that EUPS induced DC maturation.
26572415	6	10	theme	lymphocyte	749:758	arg1	proliferation					760:772	lymphocyte proliferation	749:772	lymphocyte proliferation	749:772	Furthermore, EUPS also significantly enhanced lymphocyte proliferation and significantly enhanced cytokine (IL-4 and IFN-γ) production.
26572415	7	11	theme	IgG2a	946:950	arg1	IgG					935:937	the FMDV-specific IgG	917:937	the FMDV-specific IgG	917:937	In in vivo experiments, our data showed that EUPS could significantly enhance the FMDV-specific IgG, IgG1, IgG2a, and IgG2b antibody titers and T cell proliferation.
26572415	7	11	theme	IgG2a	946:950	arg1	titers					972:977	IgG1, IgG2a, and IgG2b antibody titers	940:977	IgG1, IgG2a, and IgG2b antibody titers	940:977	In in vivo experiments, our data showed that EUPS could significantly enhance the FMDV-specific IgG, IgG1, IgG2a, and IgG2b antibody titers and T cell proliferation.
26572415	5	12	theme	DC	688:689	arg1	maturation					691:700	DC maturation	688:700	DC maturation	688:700	In in vitro experiments, EUPS (1.2-75 μg/mL) treatment of dendritic cells (DC) increased their surface expression of MHC I/II, CD80, CD40, and CD86 and indicated that EUPS induced DC maturation.
26572415	1	13	theme	immunomodulatory	191:206	arg1	effects					208:214	immunomodulatory effects	191:214	immunomodulatory effects	191:214	The aim of this study is to investigate the physicochemical properties, monosaccharide composition and immunomodulatory effects of Eucommia ulmoides Oliv.
26572415	5	14	theme	MHC	625:627	arg1	CD40					641:644	CD40	641:644	CD40	641:644	In in vitro experiments, EUPS (1.2-75 μg/mL) treatment of dendritic cells (DC) increased their surface expression of MHC I/II, CD80, CD40, and CD86 and indicated that EUPS induced DC maturation.
26572415	5	14	theme	MHC	625:627	arg1	CD86					651:654	CD86	651:654	CD86	651:654	In in vitro experiments, EUPS (1.2-75 μg/mL) treatment of dendritic cells (DC) increased their surface expression of MHC I/II, CD80, CD40, and CD86 and indicated that EUPS induced DC maturation.
26572415	5	14	theme	MHC	625:627	arg1	CD80					635:638	CD80	635:638	CD80	635:638	In in vitro experiments, EUPS (1.2-75 μg/mL) treatment of dendritic cells (DC) increased their surface expression of MHC I/II, CD80, CD40, and CD86 and indicated that EUPS induced DC maturation.
26572415	5	14	theme	MHC	625:627	arg1	I/II					629:632	MHC I/II	625:632	MHC I/II	625:632	In in vitro experiments, EUPS (1.2-75 μg/mL) treatment of dendritic cells (DC) increased their surface expression of MHC I/II, CD80, CD40, and CD86 and indicated that EUPS induced DC maturation.
26572415	7	15	theme	cell	985:988	arg1	proliferation					990:1002	T cell proliferation	983:1002	T cell proliferation	983:1002	In in vivo experiments, our data showed that EUPS could significantly enhance the FMDV-specific IgG, IgG1, IgG2a, and IgG2b antibody titers and T cell proliferation.
26572415	7	15	theme	cell	985:988	arg1	IgG					935:937	the FMDV-specific IgG	917:937	the FMDV-specific IgG	917:937	In in vivo experiments, our data showed that EUPS could significantly enhance the FMDV-specific IgG, IgG1, IgG2a, and IgG2b antibody titers and T cell proliferation.
26572415	6	16	theme	cytokine	801:808	arg1	production					827:836	cytokine (IL-4 and IFN-γ) production	801:836	cytokine (IL-4 and IFN-γ) production	801:836	Furthermore, EUPS also significantly enhanced lymphocyte proliferation and significantly enhanced cytokine (IL-4 and IFN-γ) production.
26572415	4	17	with	fucose	395:400	arg1	mass					443:446	a relative mass	432:446	a relative mass of 36.6%, 16.6%, 14.2%, 15.7%, 9.5% and 7.4%, respectively	432:505	The monosaccharide components of EUPS were glucose, fructose, mannose, fucose, galactose and arabinose with a relative mass of 36.6%, 16.6%, 14.2%, 15.7%, 9.5% and 7.4%, respectively.
26572415	4	18	theme	%	455:455	arg1	mass					443:446	a relative mass	432:446	a relative mass of 36.6%, 16.6%, 14.2%, 15.7%, 9.5% and 7.4%, respectively	432:505	The monosaccharide components of EUPS were glucose, fructose, mannose, fucose, galactose and arabinose with a relative mass of 36.6%, 16.6%, 14.2%, 15.7%, 9.5% and 7.4%, respectively.
26572415	5	19	theme	EUPS	533:536	arg1	treatment					553:561	EUPS (1.2-75 μg/mL) treatment	533:561	EUPS (1.2-75 μg/mL) treatment of dendritic cells (DC)	533:585	In in vitro experiments, EUPS (1.2-75 μg/mL) treatment of dendritic cells (DC) increased their surface expression of MHC I/II, CD80, CD40, and CD86 and indicated that EUPS induced DC maturation.
26572415	1	20	theme	study	104:108	arg1	aim					92:94	The aim	88:94	The aim of this study	88:108	The aim of this study is to investigate the physicochemical properties, monosaccharide composition and immunomodulatory effects of Eucommia ulmoides Oliv.
26572415	7	21	dep	in	842:843	arg1	vivo					845:848	vivo	845:848	vivo	845:848	In in vivo experiments, our data showed that EUPS could significantly enhance the FMDV-specific IgG, IgG1, IgG2a, and IgG2b antibody titers and T cell proliferation.
26572415	1	22	theme	Eucommia	219:226	arg1	Oliv					237:240	Eucommia ulmoides Oliv	219:240	Eucommia ulmoides Oliv	219:240	The aim of this study is to investigate the physicochemical properties, monosaccharide composition and immunomodulatory effects of Eucommia ulmoides Oliv.
26572415	4	23	with	mannose	386:392	arg1	mass					443:446	a relative mass	432:446	a relative mass of 36.6%, 16.6%, 14.2%, 15.7%, 9.5% and 7.4%, respectively	432:505	The monosaccharide components of EUPS were glucose, fructose, mannose, fucose, galactose and arabinose with a relative mass of 36.6%, 16.6%, 14.2%, 15.7%, 9.5% and 7.4%, respectively.
26572415	1	24	theme	ulmoides	228:235	arg1	Oliv					237:240	Eucommia ulmoides Oliv	219:240	Eucommia ulmoides Oliv	219:240	The aim of this study is to investigate the physicochemical properties, monosaccharide composition and immunomodulatory effects of Eucommia ulmoides Oliv.
26572415	0	25	theme	immunoenhancement	21:37	arg1	activities					39:48	immunoenhancement activities	21:48	immunoenhancement activities	21:48	Characterization and immunoenhancement activities of Eucommia ulmoides polysaccharides.
26572415	1	26	theme	Oliv	237:240	arg1	properties					148:157	the physicochemical properties	128:157	the physicochemical properties	128:157	The aim of this study is to investigate the physicochemical properties, monosaccharide composition and immunomodulatory effects of Eucommia ulmoides Oliv.
26572415	1	26	theme	Oliv	237:240	arg1	composition					175:185	monosaccharide composition	160:185	monosaccharide composition	160:185	The aim of this study is to investigate the physicochemical properties, monosaccharide composition and immunomodulatory effects of Eucommia ulmoides Oliv.
26572415	1	26	theme	Oliv	237:240	arg1	effects					208:214	immunomodulatory effects	191:214	immunomodulatory effects	191:214	The aim of this study is to investigate the physicochemical properties, monosaccharide composition and immunomodulatory effects of Eucommia ulmoides Oliv.
26572415	0	27	theme	Eucommia	53:60	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and immunoenhancement activities of Eucommia ulmoides polysaccharides.
26572415	0	27	theme	Eucommia	53:60	arg1	activities					39:48	immunoenhancement activities	21:48	immunoenhancement activities	21:48	Characterization and immunoenhancement activities of Eucommia ulmoides polysaccharides.
26572415	4	28	theme	%	482:482	arg1	mass					443:446	a relative mass	432:446	a relative mass of 36.6%, 16.6%, 14.2%, 15.7%, 9.5% and 7.4%, respectively	432:505	The monosaccharide components of EUPS were glucose, fructose, mannose, fucose, galactose and arabinose with a relative mass of 36.6%, 16.6%, 14.2%, 15.7%, 9.5% and 7.4%, respectively.
26572415	4	29	with	galactose	403:411	arg1	mass					443:446	a relative mass	432:446	a relative mass of 36.6%, 16.6%, 14.2%, 15.7%, 9.5% and 7.4%, respectively	432:505	The monosaccharide components of EUPS were glucose, fructose, mannose, fucose, galactose and arabinose with a relative mass of 36.6%, 16.6%, 14.2%, 15.7%, 9.5% and 7.4%, respectively.
26572415	3	30	theme	EUPS	296:299	arg1	Mw					289:290	Mw	289:290	Mw	289:290	The average molecular weight (Mw) of EUPS was 11.4632 × 10(5)Da.
26572415	3	30	theme	EUPS	296:299	arg1	Da					320:321	11.4632 × 10(5)Da	305:321	11.4632 × 10(5)Da	305:321	The average molecular weight (Mw) of EUPS was 11.4632 × 10(5)Da.
26572415	3	30	theme	EUPS	296:299	arg1	weight					281:286	The average molecular weight	259:286	The average molecular weight (Mw) of EUPS	259:299	The average molecular weight (Mw) of EUPS was 11.4632 × 10(5)Da.
26572415	4	31	theme	%	469:469	arg1	mass					443:446	a relative mass	432:446	a relative mass of 36.6%, 16.6%, 14.2%, 15.7%, 9.5% and 7.4%, respectively	432:505	The monosaccharide components of EUPS were glucose, fructose, mannose, fucose, galactose and arabinose with a relative mass of 36.6%, 16.6%, 14.2%, 15.7%, 9.5% and 7.4%, respectively.
26572415	4	32	theme	relative	434:441	arg1	mass					443:446	a relative mass	432:446	a relative mass of 36.6%, 16.6%, 14.2%, 15.7%, 9.5% and 7.4%, respectively	432:505	The monosaccharide components of EUPS were glucose, fructose, mannose, fucose, galactose and arabinose with a relative mass of 36.6%, 16.6%, 14.2%, 15.7%, 9.5% and 7.4%, respectively.
26572415	4	33	theme	monosaccharide	328:341	arg1	components					343:352	The monosaccharide components	324:352	The monosaccharide components of EUPS	324:360	The monosaccharide components of EUPS were glucose, fructose, mannose, fucose, galactose and arabinose with a relative mass of 36.6%, 16.6%, 14.2%, 15.7%, 9.5% and 7.4%, respectively.
26572415	4	33	theme	monosaccharide	328:341	arg1	glucose					367:373	glucose	367:373	glucose	367:373	The monosaccharide components of EUPS were glucose, fructose, mannose, fucose, galactose and arabinose with a relative mass of 36.6%, 16.6%, 14.2%, 15.7%, 9.5% and 7.4%, respectively.
26572415	7	34	theme	FMDV-specific	921:933	arg1	proliferation					990:1002	T cell proliferation	983:1002	T cell proliferation	983:1002	In in vivo experiments, our data showed that EUPS could significantly enhance the FMDV-specific IgG, IgG1, IgG2a, and IgG2b antibody titers and T cell proliferation.
26572415	7	34	theme	FMDV-specific	921:933	arg1	IgG					935:937	the FMDV-specific IgG	917:937	the FMDV-specific IgG	917:937	In in vivo experiments, our data showed that EUPS could significantly enhance the FMDV-specific IgG, IgG1, IgG2a, and IgG2b antibody titers and T cell proliferation.
26572415	7	34	theme	FMDV-specific	921:933	arg1	titers					972:977	IgG1, IgG2a, and IgG2b antibody titers	940:977	IgG1, IgG2a, and IgG2b antibody titers	940:977	In in vivo experiments, our data showed that EUPS could significantly enhance the FMDV-specific IgG, IgG1, IgG2a, and IgG2b antibody titers and T cell proliferation.
26572415	7	35	theme	T	983:983	arg1	proliferation					990:1002	T cell proliferation	983:1002	T cell proliferation	983:1002	In in vivo experiments, our data showed that EUPS could significantly enhance the FMDV-specific IgG, IgG1, IgG2a, and IgG2b antibody titers and T cell proliferation.
26572415	7	35	theme	T	983:983	arg1	IgG					935:937	the FMDV-specific IgG	917:937	the FMDV-specific IgG	917:937	In in vivo experiments, our data showed that EUPS could significantly enhance the FMDV-specific IgG, IgG1, IgG2a, and IgG2b antibody titers and T cell proliferation.
26572415	4	36	theme	%	476:476	arg1	mass					443:446	a relative mass	432:446	a relative mass of 36.6%, 16.6%, 14.2%, 15.7%, 9.5% and 7.4%, respectively	432:505	The monosaccharide components of EUPS were glucose, fructose, mannose, fucose, galactose and arabinose with a relative mass of 36.6%, 16.6%, 14.2%, 15.7%, 9.5% and 7.4%, respectively.
26572415	5	37	theme	dendritic	566:574	arg1	cells					576:580	dendritic cells	566:580	dendritic cells (DC)	566:585	In in vitro experiments, EUPS (1.2-75 μg/mL) treatment of dendritic cells (DC) increased their surface expression of MHC I/II, CD80, CD40, and CD86 and indicated that EUPS induced DC maturation.
26572415	5	37	theme	dendritic	566:574	arg1	DC					583:584	DC	583:584	DC	583:584	In in vitro experiments, EUPS (1.2-75 μg/mL) treatment of dendritic cells (DC) increased their surface expression of MHC I/II, CD80, CD40, and CD86 and indicated that EUPS induced DC maturation.
26572415	7	38	theme	IgG1	940:943	arg1	IgG					935:937	the FMDV-specific IgG	917:937	the FMDV-specific IgG	917:937	In in vivo experiments, our data showed that EUPS could significantly enhance the FMDV-specific IgG, IgG1, IgG2a, and IgG2b antibody titers and T cell proliferation.
26572415	7	38	theme	IgG1	940:943	arg1	titers					972:977	IgG1, IgG2a, and IgG2b antibody titers	940:977	IgG1, IgG2a, and IgG2b antibody titers	940:977	In in vivo experiments, our data showed that EUPS could significantly enhance the FMDV-specific IgG, IgG1, IgG2a, and IgG2b antibody titers and T cell proliferation.
26572415	4	39	theme	EUPS	357:360	arg1	components					343:352	The monosaccharide components	324:352	The monosaccharide components of EUPS	324:360	The monosaccharide components of EUPS were glucose, fructose, mannose, fucose, galactose and arabinose with a relative mass of 36.6%, 16.6%, 14.2%, 15.7%, 9.5% and 7.4%, respectively.
26572415	4	39	theme	EUPS	357:360	arg1	glucose					367:373	glucose	367:373	glucose	367:373	The monosaccharide components of EUPS were glucose, fructose, mannose, fucose, galactose and arabinose with a relative mass of 36.6%, 16.6%, 14.2%, 15.7%, 9.5% and 7.4%, respectively.
26572415	5	40	dep	in	511:512	arg1	vitro					514:518	vitro	514:518	vitro	514:518	In in vitro experiments, EUPS (1.2-75 μg/mL) treatment of dendritic cells (DC) increased their surface expression of MHC I/II, CD80, CD40, and CD86 and indicated that EUPS induced DC maturation.
26572415	3	41	theme	×	313:313	arg1	Da					320:321	11.4632 × 10(5)Da	305:321	11.4632 × 10(5)Da	305:321	The average molecular weight (Mw) of EUPS was 11.4632 × 10(5)Da.
26572415	3	41	theme	×	313:313	arg1	weight					281:286	The average molecular weight	259:286	The average molecular weight (Mw) of EUPS	259:299	The average molecular weight (Mw) of EUPS was 11.4632 × 10(5)Da.
26572415	1	42	theme	physicochemical	132:146	arg1	properties					148:157	the physicochemical properties	128:157	the physicochemical properties	128:157	The aim of this study is to investigate the physicochemical properties, monosaccharide composition and immunomodulatory effects of Eucommia ulmoides Oliv.
26572415	5	43	theme	I/II	629:632	arg1	expression					611:620	their surface expression	597:620	their surface expression of MHC I/II, CD80, CD40, and CD86	597:654	In in vitro experiments, EUPS (1.2-75 μg/mL) treatment of dendritic cells (DC) increased their surface expression of MHC I/II, CD80, CD40, and CD86 and indicated that EUPS induced DC maturation.
26572415	4	44	with	arabinose	417:425	arg1	mass					443:446	a relative mass	432:446	a relative mass of 36.6%, 16.6%, 14.2%, 15.7%, 9.5% and 7.4%, respectively	432:505	The monosaccharide components of EUPS were glucose, fructose, mannose, fucose, galactose and arabinose with a relative mass of 36.6%, 16.6%, 14.2%, 15.7%, 9.5% and 7.4%, respectively.
26572415	4	45	theme	%	462:462	arg1	mass					443:446	a relative mass	432:446	a relative mass of 36.6%, 16.6%, 14.2%, 15.7%, 9.5% and 7.4%, respectively	432:505	The monosaccharide components of EUPS were glucose, fructose, mannose, fucose, galactose and arabinose with a relative mass of 36.6%, 16.6%, 14.2%, 15.7%, 9.5% and 7.4%, respectively.
26572415	7	46	theme	in	842:843	arg1	experiments					850:860	in vivo experiments	842:860	in vivo experiments	842:860	In in vivo experiments, our data showed that EUPS could significantly enhance the FMDV-specific IgG, IgG1, IgG2a, and IgG2b antibody titers and T cell proliferation.
26572415	1	47	theme	monosaccharide	160:173	arg1	composition					175:185	monosaccharide composition	160:185	monosaccharide composition	160:185	The aim of this study is to investigate the physicochemical properties, monosaccharide composition and immunomodulatory effects of Eucommia ulmoides Oliv.
25263889	1	0	theme	PPy	159:161	arg1	KC					182:183	KC	182:183	KC	182:183	A novel nano-bio composite polypyrrole (PPy)/kappa-carrageenan(KC) was fabricated and characterized for application as a cathode catalyst in a microbial fuel cell (MFC).
25263889	1	0	theme	PPy	159:161	arg1	catalyst					248:255	a cathode catalyst	238:255	a cathode catalyst in a microbial fuel cell (MFC)	238:286	A novel nano-bio composite polypyrrole (PPy)/kappa-carrageenan(KC) was fabricated and characterized for application as a cathode catalyst in a microbial fuel cell (MFC).
25263889	1	0	theme	PPy	159:161	arg1	/kappa-carrageenan					163:180	A novel nano-bio composite polypyrrole (PPy)/kappa-carrageenan	119:180	A novel nano-bio composite polypyrrole (PPy)/kappa-carrageenan(KC)	119:184	A novel nano-bio composite polypyrrole (PPy)/kappa-carrageenan(KC) was fabricated and characterized for application as a cathode catalyst in a microbial fuel cell (MFC).
25263889	6	1	theme	electrode	1067:1075	arg1	system					1077:1082	an alternative electrode system	1052:1082	an alternative electrode system for MFCs	1052:1091	The efficiency of the PPy/KC electrode system is slightly lower than a Pt electrode (79.9 mW/m(2)) but due to the high cost of Pt electrodes, the PPy/KC electrode system has potential to be an alternative electrode system for MFCs.
25263889	1	2	theme	cathode	240:246	arg1	catalyst					248:255	a cathode catalyst	238:255	a cathode catalyst in a microbial fuel cell (MFC)	238:286	A novel nano-bio composite polypyrrole (PPy)/kappa-carrageenan(KC) was fabricated and characterized for application as a cathode catalyst in a microbial fuel cell (MFC).
25263889	1	2	theme	cathode	240:246	arg1	/kappa-carrageenan					163:180	A novel nano-bio composite polypyrrole (PPy)/kappa-carrageenan	119:180	A novel nano-bio composite polypyrrole (PPy)/kappa-carrageenan(KC)	119:184	A novel nano-bio composite polypyrrole (PPy)/kappa-carrageenan(KC) was fabricated and characterized for application as a cathode catalyst in a microbial fuel cell (MFC).
25263889	6	3	theme	Pt	933:934	arg1	electrode					936:944	a Pt electrode	931:944	a Pt electrode (79.9 mW/m(2))	931:959	The efficiency of the PPy/KC electrode system is slightly lower than a Pt electrode (79.9 mW/m(2)) but due to the high cost of Pt electrodes, the PPy/KC electrode system has potential to be an alternative electrode system for MFCs.
25263889	0	4	theme	fuel	107:110	arg1	cells					112:116	microbial fuel cells	97:116	microbial fuel cells	97:116	Synthesis and application of polypyrrole/carrageenan nano-bio composite as a cathode catalyst in microbial fuel cells.
25263889	6	5	theme	electrodes	992:1001	arg1	cost					981:984	the high cost	972:984	the high cost of Pt electrodes	972:1001	The efficiency of the PPy/KC electrode system is slightly lower than a Pt electrode (79.9 mW/m(2)) but due to the high cost of Pt electrodes, the PPy/KC electrode system has potential to be an alternative electrode system for MFCs.
25263889	0	6	theme	microbial	97:105	arg1	cells					112:116	microbial fuel cells	97:116	microbial fuel cells	97:116	Synthesis and application of polypyrrole/carrageenan nano-bio composite as a cathode catalyst in microbial fuel cells.
25263889	4	7	theme	cathode	618:624	arg1	composites					503:512	The PPy/KC nano-bio composites	483:512	The PPy/KC nano-bio composites	483:512	The PPy/KC nano-bio composites were then studied as an electrode material, due to their oxygen reduction reaction (ORR) ability as the cathode catalyst in the MFC and the results were compared with platinum (Pt) as the most common cathode catalyst.
25263889	4	7	theme	cathode	618:624	arg1	catalyst					626:633	the cathode catalyst	614:633	the cathode catalyst in the MFC	614:644	The PPy/KC nano-bio composites were then studied as an electrode material, due to their oxygen reduction reaction (ORR) ability as the cathode catalyst in the MFC and the results were compared with platinum (Pt) as the most common cathode catalyst.
25263889	2	8	theme	resolution	294:303	arg1	SEM					305:307	High resolution SEM	289:307	High resolution SEM	289:307	High resolution SEM and TEM verified the bud-like shape and uniform distribution of the PPy in the KC matrix.
25263889	4	9	theme	nano-bio	494:501	arg1	material					548:555	an electrode material	535:555	an electrode material	535:555	The PPy/KC nano-bio composites were then studied as an electrode material, due to their oxygen reduction reaction (ORR) ability as the cathode catalyst in the MFC and the results were compared with platinum (Pt) as the most common cathode catalyst.
25263889	4	9	theme	nano-bio	494:501	arg1	composites					503:512	The PPy/KC nano-bio composites	483:512	The PPy/KC nano-bio composites	483:512	The PPy/KC nano-bio composites were then studied as an electrode material, due to their oxygen reduction reaction (ORR) ability as the cathode catalyst in the MFC and the results were compared with platinum (Pt) as the most common cathode catalyst.
25263889	4	9	theme	nano-bio	494:501	arg1	catalyst					626:633	the cathode catalyst	614:633	the cathode catalyst in the MFC	614:644	The PPy/KC nano-bio composites were then studied as an electrode material, due to their oxygen reduction reaction (ORR) ability as the cathode catalyst in the MFC and the results were compared with platinum (Pt) as the most common cathode catalyst.
25263889	3	10	contain	has	423:425	arg1	XRD					418:420	XRD	418:420	XRD	418:420	X-ray diffraction (XRD) has approved the amorphous structure of the PPy/KC as well.
25263889	3	10	contain	has	423:425	arg2	structure					450:458	approved the amorphous structure	427:458	approved the amorphous structure of the PPy/KC	427:472	X-ray diffraction (XRD) has approved the amorphous structure of the PPy/KC as well.
25263889	3	10	contain	has	423:425	arg1	diffraction					405:415	X-ray diffraction	399:415	X-ray diffraction (XRD)	399:421	X-ray diffraction (XRD) has approved the amorphous structure of the PPy/KC as well.
25263889	6	11	theme	electrode	1015:1023	arg1	system					1025:1030	the PPy/KC electrode system	1004:1030	the PPy/KC electrode system	1004:1030	The efficiency of the PPy/KC electrode system is slightly lower than a Pt electrode (79.9 mW/m(2)) but due to the high cost of Pt electrodes, the PPy/KC electrode system has potential to be an alternative electrode system for MFCs.
25263889	6	12	contain	has	1032:1034	arg2	potential					1036:1044	potential	1036:1044	potential to be an alternative electrode system for MFCs	1036:1091	The efficiency of the PPy/KC electrode system is slightly lower than a Pt electrode (79.9 mW/m(2)) but due to the high cost of Pt electrodes, the PPy/KC electrode system has potential to be an alternative electrode system for MFCs.
25263889	6	12	contain	has	1032:1034	arg1	system					1025:1030	the PPy/KC electrode system	1004:1030	the PPy/KC electrode system	1004:1030	The efficiency of the PPy/KC electrode system is slightly lower than a Pt electrode (79.9 mW/m(2)) but due to the high cost of Pt electrodes, the PPy/KC electrode system has potential to be an alternative electrode system for MFCs.
25263889	6	13	theme	electrode	891:899	arg1	system					901:906	the PPy/KC electrode system	880:906	the PPy/KC electrode system	880:906	The efficiency of the PPy/KC electrode system is slightly lower than a Pt electrode (79.9 mW/m(2)) but due to the high cost of Pt electrodes, the PPy/KC electrode system has potential to be an alternative electrode system for MFCs.
25263889	6	14	theme	PPy/KC	1008:1013	arg1	system					1025:1030	the PPy/KC electrode system	1004:1030	the PPy/KC electrode system	1004:1030	The efficiency of the PPy/KC electrode system is slightly lower than a Pt electrode (79.9 mW/m(2)) but due to the high cost of Pt electrodes, the PPy/KC electrode system has potential to be an alternative electrode system for MFCs.
25263889	3	15	theme	X-ray	399:403	arg1	XRD					418:420	XRD	418:420	XRD	418:420	X-ray diffraction (XRD) has approved the amorphous structure of the PPy/KC as well.
25263889	3	15	theme	X-ray	399:403	arg1	diffraction					405:415	X-ray diffraction	399:415	X-ray diffraction (XRD)	399:421	X-ray diffraction (XRD) has approved the amorphous structure of the PPy/KC as well.
25263889	3	16	theme	PPy/KC	467:472	arg1	structure					450:458	approved the amorphous structure	427:458	approved the amorphous structure of the PPy/KC	427:472	X-ray diffraction (XRD) has approved the amorphous structure of the PPy/KC as well.
25263889	4	17	theme	reaction	588:595	arg1	ability					603:609	their oxygen reduction reaction (ORR) ability	565:609	their oxygen reduction reaction (ORR) ability	565:609	The PPy/KC nano-bio composites were then studied as an electrode material, due to their oxygen reduction reaction (ORR) ability as the cathode catalyst in the MFC and the results were compared with platinum (Pt) as the most common cathode catalyst.
25263889	2	18	theme	High	289:292	arg1	resolution					294:303	High resolution	289:303	High resolution SEM	289:307	High resolution SEM and TEM verified the bud-like shape and uniform distribution of the PPy in the KC matrix.
25263889	2	19	theme	uniform	349:355	arg1	distribution					357:368	uniform distribution	349:368	uniform distribution	349:368	High resolution SEM and TEM verified the bud-like shape and uniform distribution of the PPy in the KC matrix.
25263889	1	20	theme	microbial	262:270	arg1	cell					277:280	microbial fuel cell	262:280	a microbial fuel cell (MFC)	260:286	A novel nano-bio composite polypyrrole (PPy)/kappa-carrageenan(KC) was fabricated and characterized for application as a cathode catalyst in a microbial fuel cell (MFC).
25263889	1	20	theme	microbial	262:270	arg1	MFC					283:285	MFC	283:285	MFC	283:285	A novel nano-bio composite polypyrrole (PPy)/kappa-carrageenan(KC) was fabricated and characterized for application as a cathode catalyst in a microbial fuel cell (MFC).
25263889	2	21	from	shape	339:343	arg1	matrix					391:396	the KC matrix	384:396	the KC matrix	384:396	High resolution SEM and TEM verified the bud-like shape and uniform distribution of the PPy in the KC matrix.
25263889	4	22	theme	ORR	598:600	arg1	ability					603:609	their oxygen reduction reaction (ORR) ability	565:609	their oxygen reduction reaction (ORR) ability	565:609	The PPy/KC nano-bio composites were then studied as an electrode material, due to their oxygen reduction reaction (ORR) ability as the cathode catalyst in the MFC and the results were compared with platinum (Pt) as the most common cathode catalyst.
25263889	5	23	theme	produced	736:743	arg1	mW/m					782:785	72.1 mW/m	777:785	72.1 mW/m(2)	777:788	The produced power density of the PPy/KC was 72.1 mW/m(2) while it was 46.8 mW/m(2) and 28.8 mW/m(2) for KC and PPy individually.
25263889	5	23	theme	produced	736:743	arg1	density					751:757	The produced power density	732:757	The produced power density of the PPy/KC	732:771	The produced power density of the PPy/KC was 72.1 mW/m(2) while it was 46.8 mW/m(2) and 28.8 mW/m(2) for KC and PPy individually.
25263889	1	24	theme	fuel	272:275	arg1	cell					277:280	microbial fuel cell	262:280	a microbial fuel cell (MFC)	260:286	A novel nano-bio composite polypyrrole (PPy)/kappa-carrageenan(KC) was fabricated and characterized for application as a cathode catalyst in a microbial fuel cell (MFC).
25263889	1	24	theme	fuel	272:275	arg1	MFC					283:285	MFC	283:285	MFC	283:285	A novel nano-bio composite polypyrrole (PPy)/kappa-carrageenan(KC) was fabricated and characterized for application as a cathode catalyst in a microbial fuel cell (MFC).
25263889	6	25	theme	PPy/KC	884:889	arg1	system					901:906	the PPy/KC electrode system	880:906	the PPy/KC electrode system	880:906	The efficiency of the PPy/KC electrode system is slightly lower than a Pt electrode (79.9 mW/m(2)) but due to the high cost of Pt electrodes, the PPy/KC electrode system has potential to be an alternative electrode system for MFCs.
25263889	2	26	theme	KC	388:389	arg1	matrix					391:396	the KC matrix	384:396	the KC matrix	384:396	High resolution SEM and TEM verified the bud-like shape and uniform distribution of the PPy in the KC matrix.
25263889	6	27	theme	system	901:906	arg1	efficiency					866:875	The efficiency	862:875	The efficiency of the PPy/KC electrode system	862:906	The efficiency of the PPy/KC electrode system is slightly lower than a Pt electrode (79.9 mW/m(2)) but due to the high cost of Pt electrodes, the PPy/KC electrode system has potential to be an alternative electrode system for MFCs.
25263889	6	27	theme	system	901:906	arg1	lower					920:924	lower	920:924	lower	920:924	The efficiency of the PPy/KC electrode system is slightly lower than a Pt electrode (79.9 mW/m(2)) but due to the high cost of Pt electrodes, the PPy/KC electrode system has potential to be an alternative electrode system for MFCs.
25263889	0	28	theme	nano-bio	53:60	arg1	composite					62:70	polypyrrole/carrageenan nano-bio composite	29:70	polypyrrole/carrageenan nano-bio composite	29:70	Synthesis and application of polypyrrole/carrageenan nano-bio composite as a cathode catalyst in microbial fuel cells.
25263889	3	29	theme	amorphous	440:448	arg1	structure					450:458	approved the amorphous structure	427:458	approved the amorphous structure of the PPy/KC	427:472	X-ray diffraction (XRD) has approved the amorphous structure of the PPy/KC as well.
25263889	0	30	from	catalyst	85:92	arg1	cells					112:116	microbial fuel cells	97:116	microbial fuel cells	97:116	Synthesis and application of polypyrrole/carrageenan nano-bio composite as a cathode catalyst in microbial fuel cells.
25263889	4	31	theme	common	707:712	arg1	catalyst					722:729	the most common cathode catalyst	698:729	the most common cathode catalyst	698:729	The PPy/KC nano-bio composites were then studied as an electrode material, due to their oxygen reduction reaction (ORR) ability as the cathode catalyst in the MFC and the results were compared with platinum (Pt) as the most common cathode catalyst.
25263889	4	32	from	catalyst	626:633	arg1	MFC					642:644	the MFC	638:644	the MFC	638:644	The PPy/KC nano-bio composites were then studied as an electrode material, due to their oxygen reduction reaction (ORR) ability as the cathode catalyst in the MFC and the results were compared with platinum (Pt) as the most common cathode catalyst.
25263889	2	33	theme	PPy	377:379	arg1	distribution					357:368	uniform distribution	349:368	uniform distribution	349:368	High resolution SEM and TEM verified the bud-like shape and uniform distribution of the PPy in the KC matrix.
25263889	2	33	theme	PPy	377:379	arg1	shape					339:343	the bud-like shape	326:343	the bud-like shape	326:343	High resolution SEM and TEM verified the bud-like shape and uniform distribution of the PPy in the KC matrix.
25263889	0	34	theme	polypyrrole/carrageenan	29:51	arg1	composite					62:70	polypyrrole/carrageenan nano-bio composite	29:70	polypyrrole/carrageenan nano-bio composite	29:70	Synthesis and application of polypyrrole/carrageenan nano-bio composite as a cathode catalyst in microbial fuel cells.
25263889	1	35	from	catalyst	248:255	arg1	cell					277:280	microbial fuel cell	262:280	a microbial fuel cell (MFC)	260:286	A novel nano-bio composite polypyrrole (PPy)/kappa-carrageenan(KC) was fabricated and characterized for application as a cathode catalyst in a microbial fuel cell (MFC).
25263889	1	35	from	catalyst	248:255	arg1	MFC					283:285	MFC	283:285	MFC	283:285	A novel nano-bio composite polypyrrole (PPy)/kappa-carrageenan(KC) was fabricated and characterized for application as a cathode catalyst in a microbial fuel cell (MFC).
25263889	6	36	theme	Pt	989:990	arg1	electrodes					992:1001	Pt electrodes	989:1001	Pt electrodes	989:1001	The efficiency of the PPy/KC electrode system is slightly lower than a Pt electrode (79.9 mW/m(2)) but due to the high cost of Pt electrodes, the PPy/KC electrode system has potential to be an alternative electrode system for MFCs.
25263889	4	37	theme	oxygen	571:576	arg1	ability					603:609	their oxygen reduction reaction (ORR) ability	565:609	their oxygen reduction reaction (ORR) ability	565:609	The PPy/KC nano-bio composites were then studied as an electrode material, due to their oxygen reduction reaction (ORR) ability as the cathode catalyst in the MFC and the results were compared with platinum (Pt) as the most common cathode catalyst.
25263889	4	38	theme	reduction	578:586	arg1	ability					603:609	their oxygen reduction reaction (ORR) ability	565:609	their oxygen reduction reaction (ORR) ability	565:609	The PPy/KC nano-bio composites were then studied as an electrode material, due to their oxygen reduction reaction (ORR) ability as the cathode catalyst in the MFC and the results were compared with platinum (Pt) as the most common cathode catalyst.
25263889	4	39	theme	PPy/KC	487:492	arg1	material					548:555	an electrode material	535:555	an electrode material	535:555	The PPy/KC nano-bio composites were then studied as an electrode material, due to their oxygen reduction reaction (ORR) ability as the cathode catalyst in the MFC and the results were compared with platinum (Pt) as the most common cathode catalyst.
25263889	4	39	theme	PPy/KC	487:492	arg1	composites					503:512	The PPy/KC nano-bio composites	483:512	The PPy/KC nano-bio composites	483:512	The PPy/KC nano-bio composites were then studied as an electrode material, due to their oxygen reduction reaction (ORR) ability as the cathode catalyst in the MFC and the results were compared with platinum (Pt) as the most common cathode catalyst.
25263889	4	39	theme	PPy/KC	487:492	arg1	catalyst					626:633	the cathode catalyst	614:633	the cathode catalyst in the MFC	614:644	The PPy/KC nano-bio composites were then studied as an electrode material, due to their oxygen reduction reaction (ORR) ability as the cathode catalyst in the MFC and the results were compared with platinum (Pt) as the most common cathode catalyst.
25263889	0	40	theme	composite	62:70	arg1	application					14:24	application	14:24	application	14:24	Synthesis and application of polypyrrole/carrageenan nano-bio composite as a cathode catalyst in microbial fuel cells.
25263889	0	40	theme	composite	62:70	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis and application of polypyrrole/carrageenan nano-bio composite as a cathode catalyst in microbial fuel cells.
25263889	6	41	theme	alternative	1055:1065	arg1	system					1077:1082	an alternative electrode system	1052:1082	an alternative electrode system for MFCs	1052:1091	The efficiency of the PPy/KC electrode system is slightly lower than a Pt electrode (79.9 mW/m(2)) but due to the high cost of Pt electrodes, the PPy/KC electrode system has potential to be an alternative electrode system for MFCs.
25263889	4	42	theme	cathode	714:720	arg1	catalyst					722:729	the most common cathode catalyst	698:729	the most common cathode catalyst	698:729	The PPy/KC nano-bio composites were then studied as an electrode material, due to their oxygen reduction reaction (ORR) ability as the cathode catalyst in the MFC and the results were compared with platinum (Pt) as the most common cathode catalyst.
25263889	1	43	theme	novel	121:125	arg1	KC					182:183	KC	182:183	KC	182:183	A novel nano-bio composite polypyrrole (PPy)/kappa-carrageenan(KC) was fabricated and characterized for application as a cathode catalyst in a microbial fuel cell (MFC).
25263889	1	43	theme	novel	121:125	arg1	catalyst					248:255	a cathode catalyst	238:255	a cathode catalyst in a microbial fuel cell (MFC)	238:286	A novel nano-bio composite polypyrrole (PPy)/kappa-carrageenan(KC) was fabricated and characterized for application as a cathode catalyst in a microbial fuel cell (MFC).
25263889	1	43	theme	novel	121:125	arg1	/kappa-carrageenan					163:180	A novel nano-bio composite polypyrrole (PPy)/kappa-carrageenan	119:180	A novel nano-bio composite polypyrrole (PPy)/kappa-carrageenan(KC)	119:184	A novel nano-bio composite polypyrrole (PPy)/kappa-carrageenan(KC) was fabricated and characterized for application as a cathode catalyst in a microbial fuel cell (MFC).
25263889	6	44	theme	high	976:979	arg1	cost					981:984	the high cost	972:984	the high cost of Pt electrodes	972:1001	The efficiency of the PPy/KC electrode system is slightly lower than a Pt electrode (79.9 mW/m(2)) but due to the high cost of Pt electrodes, the PPy/KC electrode system has potential to be an alternative electrode system for MFCs.
25263889	2	45	from	distribution	357:368	arg1	matrix					391:396	the KC matrix	384:396	the KC matrix	384:396	High resolution SEM and TEM verified the bud-like shape and uniform distribution of the PPy in the KC matrix.
25263889	1	46	theme	nano-bio	127:134	arg1	KC					182:183	KC	182:183	KC	182:183	A novel nano-bio composite polypyrrole (PPy)/kappa-carrageenan(KC) was fabricated and characterized for application as a cathode catalyst in a microbial fuel cell (MFC).
25263889	1	46	theme	nano-bio	127:134	arg1	catalyst					248:255	a cathode catalyst	238:255	a cathode catalyst in a microbial fuel cell (MFC)	238:286	A novel nano-bio composite polypyrrole (PPy)/kappa-carrageenan(KC) was fabricated and characterized for application as a cathode catalyst in a microbial fuel cell (MFC).
25263889	1	46	theme	nano-bio	127:134	arg1	/kappa-carrageenan					163:180	A novel nano-bio composite polypyrrole (PPy)/kappa-carrageenan	119:180	A novel nano-bio composite polypyrrole (PPy)/kappa-carrageenan(KC)	119:184	A novel nano-bio composite polypyrrole (PPy)/kappa-carrageenan(KC) was fabricated and characterized for application as a cathode catalyst in a microbial fuel cell (MFC).
25263889	5	47	theme	PPy/KC	766:771	arg1	mW/m					782:785	72.1 mW/m	777:785	72.1 mW/m(2)	777:788	The produced power density of the PPy/KC was 72.1 mW/m(2) while it was 46.8 mW/m(2) and 28.8 mW/m(2) for KC and PPy individually.
25263889	5	47	theme	PPy/KC	766:771	arg1	density					751:757	The produced power density	732:757	The produced power density of the PPy/KC	732:771	The produced power density of the PPy/KC was 72.1 mW/m(2) while it was 46.8 mW/m(2) and 28.8 mW/m(2) for KC and PPy individually.
25263889	2	48	from	PPy	377:379	arg1	matrix					391:396	the KC matrix	384:396	the KC matrix	384:396	High resolution SEM and TEM verified the bud-like shape and uniform distribution of the PPy in the KC matrix.
25263889	1	49	theme	composite	136:144	arg1	KC					182:183	KC	182:183	KC	182:183	A novel nano-bio composite polypyrrole (PPy)/kappa-carrageenan(KC) was fabricated and characterized for application as a cathode catalyst in a microbial fuel cell (MFC).
25263889	1	49	theme	composite	136:144	arg1	catalyst					248:255	a cathode catalyst	238:255	a cathode catalyst in a microbial fuel cell (MFC)	238:286	A novel nano-bio composite polypyrrole (PPy)/kappa-carrageenan(KC) was fabricated and characterized for application as a cathode catalyst in a microbial fuel cell (MFC).
25263889	1	49	theme	composite	136:144	arg1	/kappa-carrageenan					163:180	A novel nano-bio composite polypyrrole (PPy)/kappa-carrageenan	119:180	A novel nano-bio composite polypyrrole (PPy)/kappa-carrageenan(KC)	119:184	A novel nano-bio composite polypyrrole (PPy)/kappa-carrageenan(KC) was fabricated and characterized for application as a cathode catalyst in a microbial fuel cell (MFC).
25263889	4	50	theme	electrode	538:546	arg1	material					548:555	an electrode material	535:555	an electrode material	535:555	The PPy/KC nano-bio composites were then studied as an electrode material, due to their oxygen reduction reaction (ORR) ability as the cathode catalyst in the MFC and the results were compared with platinum (Pt) as the most common cathode catalyst.
25263889	4	50	theme	electrode	538:546	arg1	composites					503:512	The PPy/KC nano-bio composites	483:512	The PPy/KC nano-bio composites	483:512	The PPy/KC nano-bio composites were then studied as an electrode material, due to their oxygen reduction reaction (ORR) ability as the cathode catalyst in the MFC and the results were compared with platinum (Pt) as the most common cathode catalyst.
25263889	0	51	theme	cathode	77:83	arg1	catalyst					85:92	a cathode catalyst	75:92	a cathode catalyst in microbial fuel cells	75:116	Synthesis and application of polypyrrole/carrageenan nano-bio composite as a cathode catalyst in microbial fuel cells.
25263889	5	52	theme	power	745:749	arg1	mW/m					782:785	72.1 mW/m	777:785	72.1 mW/m(2)	777:788	The produced power density of the PPy/KC was 72.1 mW/m(2) while it was 46.8 mW/m(2) and 28.8 mW/m(2) for KC and PPy individually.
25263889	5	52	theme	power	745:749	arg1	density					751:757	The produced power density	732:757	The produced power density of the PPy/KC	732:771	The produced power density of the PPy/KC was 72.1 mW/m(2) while it was 46.8 mW/m(2) and 28.8 mW/m(2) for KC and PPy individually.
25263889	6	53	dep	electrode	936:944	arg1	mW/m					952:955	79.9 mW/m	947:955	79.9 mW/m(2)	947:958	The efficiency of the PPy/KC electrode system is slightly lower than a Pt electrode (79.9 mW/m(2)) but due to the high cost of Pt electrodes, the PPy/KC electrode system has potential to be an alternative electrode system for MFCs.
25263889	6	53	dep	electrode	936:944	arg1	2					957:957	2	957:957	2	957:957	The efficiency of the PPy/KC electrode system is slightly lower than a Pt electrode (79.9 mW/m(2)) but due to the high cost of Pt electrodes, the PPy/KC electrode system has potential to be an alternative electrode system for MFCs.
25263889	1	54	theme	polypyrrole	146:156	arg1	KC					182:183	KC	182:183	KC	182:183	A novel nano-bio composite polypyrrole (PPy)/kappa-carrageenan(KC) was fabricated and characterized for application as a cathode catalyst in a microbial fuel cell (MFC).
25263889	1	54	theme	polypyrrole	146:156	arg1	catalyst					248:255	a cathode catalyst	238:255	a cathode catalyst in a microbial fuel cell (MFC)	238:286	A novel nano-bio composite polypyrrole (PPy)/kappa-carrageenan(KC) was fabricated and characterized for application as a cathode catalyst in a microbial fuel cell (MFC).
25263889	1	54	theme	polypyrrole	146:156	arg1	/kappa-carrageenan					163:180	A novel nano-bio composite polypyrrole (PPy)/kappa-carrageenan	119:180	A novel nano-bio composite polypyrrole (PPy)/kappa-carrageenan(KC)	119:184	A novel nano-bio composite polypyrrole (PPy)/kappa-carrageenan(KC) was fabricated and characterized for application as a cathode catalyst in a microbial fuel cell (MFC).
25263889	3	55	theme	approved	427:434	arg1	structure					450:458	approved the amorphous structure	427:458	approved the amorphous structure of the PPy/KC	427:472	X-ray diffraction (XRD) has approved the amorphous structure of the PPy/KC as well.
25263889	4	56	theme	due	558:560	arg1	material					548:555	an electrode material	535:555	an electrode material	535:555	The PPy/KC nano-bio composites were then studied as an electrode material, due to their oxygen reduction reaction (ORR) ability as the cathode catalyst in the MFC and the results were compared with platinum (Pt) as the most common cathode catalyst.
25263889	4	56	theme	due	558:560	arg1	composites					503:512	The PPy/KC nano-bio composites	483:512	The PPy/KC nano-bio composites	483:512	The PPy/KC nano-bio composites were then studied as an electrode material, due to their oxygen reduction reaction (ORR) ability as the cathode catalyst in the MFC and the results were compared with platinum (Pt) as the most common cathode catalyst.
25263889	2	57	from	matrix	391:396	arg1	distribution					357:368	uniform distribution	349:368	uniform distribution	349:368	High resolution SEM and TEM verified the bud-like shape and uniform distribution of the PPy in the KC matrix.
25263889	2	57	from	matrix	391:396	arg1	shape					339:343	the bud-like shape	326:343	the bud-like shape	326:343	High resolution SEM and TEM verified the bud-like shape and uniform distribution of the PPy in the KC matrix.
25263889	2	58	theme	bud-like	330:337	arg1	shape					339:343	the bud-like shape	326:343	the bud-like shape	326:343	High resolution SEM and TEM verified the bud-like shape and uniform distribution of the PPy in the KC matrix.
24299777	6	0	with	arabinofucan	836:847	arg1	amounts					861:867	higher amounts	854:867	higher amounts of N-acetyl-fucosamine (FucNAc)	854:899	Especially remarkable is the composition of these EPS: an arabinofucan with higher amounts of N-acetyl-fucosamine (FucNAc) and only minor quantities of glucose.
24299777	6	0	with	arabinofucan	836:847	arg1	glucose					930:936	glucose	930:936	glucose	930:936	Especially remarkable is the composition of these EPS: an arabinofucan with higher amounts of N-acetyl-fucosamine (FucNAc) and only minor quantities of glucose.
24299777	6	0	with	arabinofucan	836:847	arg1	quantities					916:925	only minor quantities	905:925	only minor quantities of glucose	905:936	Especially remarkable is the composition of these EPS: an arabinofucan with higher amounts of N-acetyl-fucosamine (FucNAc) and only minor quantities of glucose.
24299777	6	0	with	arabinofucan	836:847	arg1	FucNAc					893:898	FucNAc	893:898	FucNAc	893:898	Especially remarkable is the composition of these EPS: an arabinofucan with higher amounts of N-acetyl-fucosamine (FucNAc) and only minor quantities of glucose.
24299777	6	0	with	arabinofucan	836:847	arg1	N-acetyl-fucosamine					872:890	N-acetyl-fucosamine	872:890	N-acetyl-fucosamine (FucNAc)	872:899	Especially remarkable is the composition of these EPS: an arabinofucan with higher amounts of N-acetyl-fucosamine (FucNAc) and only minor quantities of glucose.
24299777	5	1	theme	pevalekii	572:580	arg1	secrets					582:588	S. pevalekii secrets complex hetero-polysaccharides and acidic proteins as proteoglycan-complexes whereas the protein-free but highly sulfated EPS from S. aquatilis only	569:737	S. pevalekii secrets complex hetero-polysaccharides and acidic proteins as proteoglycan-complexes whereas the protein-free but highly sulfated EPS from S. aquatilis only	569:737	S. pevalekii secrets complex hetero-polysaccharides and acidic proteins as proteoglycan-complexes whereas the protein-free but highly sulfated EPS from S. aquatilis only consist of 4 dominant monosaccharides.
24299777	1	2	theme	e.g.	181:184	arg1	morphology					186:195	e.g. morphology	181:195	e.g. morphology	181:195	Cyanobacteria are known to be a rather diverse group of organisms regarding e.g. morphology, metabolism and composition of excreted exopolysaccharides (EPS).
24299777	5	3	dep	proteoglycan-complexes	644:665	arg1	whereas					667:673	whereas	667:673	whereas	667:673	S. pevalekii secrets complex hetero-polysaccharides and acidic proteins as proteoglycan-complexes whereas the protein-free but highly sulfated EPS from S. aquatilis only consist of 4 dominant monosaccharides.
24299777	3	4	from	strains	432:438	arg1	EPS					407:409	EPS	407:409	EPS from the unicellular strains of Synechocystis aquatilis and S. pevalekii with various methods	407:503	This work examined EPS from the unicellular strains of Synechocystis aquatilis and S. pevalekii with various methods.
24299777	2	5	from	percentage	349:358	arg1	EPS					327:329	the EPS	323:329	the EPS from only a small percentage	323:358	Considering the high number of known cyanobacterial species the EPS from only a small percentage are investigated in detail.
24299777	0	6	contain	containing	73:82	arg2	N-acetyl-fucosamine					84:102	N-acetyl-fucosamine	84:102	N-acetyl-fucosamine	84:102	Exopolysaccharides of Synechocystis aquatilis are sulfated arabinofucans containing N-acetyl-fucosamine.
24299777	0	6	contain	containing	73:82	arg1	Exopolysaccharides					0:17	Exopolysaccharides	0:17	Exopolysaccharides of Synechocystis aquatilis	0:44	Exopolysaccharides of Synechocystis aquatilis are sulfated arabinofucans containing N-acetyl-fucosamine.
24299777	0	6	contain	containing	73:82	arg1	arabinofucans					59:71	sulfated arabinofucans	50:71	sulfated arabinofucans containing N-acetyl-fucosamine	50:102	Exopolysaccharides of Synechocystis aquatilis are sulfated arabinofucans containing N-acetyl-fucosamine.
24299777	7	7	from	EPS	944:946	arg1	EPS					988:990	EPS	988:990	EPS from S. aquatilis	988:1008	Both EPS and the newly found component FucNAc in EPS from S. aquatilis extend the possible components of cyanobacterial EPS and the knowledge of heterogeneity of cyanobacterial metabolites.
24299777	7	7	from	EPS	944:946	arg1	aquatilis					1000:1008	S. aquatilis	997:1008	S. aquatilis	997:1008	Both EPS and the newly found component FucNAc in EPS from S. aquatilis extend the possible components of cyanobacterial EPS and the knowledge of heterogeneity of cyanobacterial metabolites.
24299777	7	8	theme	cyanobacterial	1044:1057	arg1	EPS					1059:1061	cyanobacterial EPS	1044:1061	cyanobacterial EPS	1044:1061	Both EPS and the newly found component FucNAc in EPS from S. aquatilis extend the possible components of cyanobacterial EPS and the knowledge of heterogeneity of cyanobacterial metabolites.
24299777	7	9	theme	heterogeneity	1084:1096	arg1	knowledge					1071:1079	the knowledge	1067:1079	the knowledge of heterogeneity of cyanobacterial metabolites	1067:1126	Both EPS and the newly found component FucNAc in EPS from S. aquatilis extend the possible components of cyanobacterial EPS and the knowledge of heterogeneity of cyanobacterial metabolites.
24299777	7	9	theme	heterogeneity	1084:1096	arg1	components					1030:1039	the possible components	1017:1039	the possible components of cyanobacterial EPS	1017:1061	Both EPS and the newly found component FucNAc in EPS from S. aquatilis extend the possible components of cyanobacterial EPS and the knowledge of heterogeneity of cyanobacterial metabolites.
24299777	5	10	theme	protein-free	679:690	arg1	EPS					712:714	the protein-free but highly sulfated EPS	675:714	acidic proteins as proteoglycan-complexes whereas the protein-free but highly sulfated EPS from S. aquatilis	625:732	S. pevalekii secrets complex hetero-polysaccharides and acidic proteins as proteoglycan-complexes whereas the protein-free but highly sulfated EPS from S. aquatilis only consist of 4 dominant monosaccharides.
24299777	2	11	theme	species	315:321	arg1	number					284:289	the high number	275:289	the high number of known cyanobacterial species	275:321	Considering the high number of known cyanobacterial species the EPS from only a small percentage are investigated in detail.
24299777	7	12	from	aquatilis	1000:1008	arg1	EPS					944:946	EPS	944:946	EPS	944:946	Both EPS and the newly found component FucNAc in EPS from S. aquatilis extend the possible components of cyanobacterial EPS and the knowledge of heterogeneity of cyanobacterial metabolites.
24299777	7	12	from	aquatilis	1000:1008	arg1	FucNAc					978:983	the newly found component FucNAc	952:983	the newly found component FucNAc in EPS from S. aquatilis	952:1008	Both EPS and the newly found component FucNAc in EPS from S. aquatilis extend the possible components of cyanobacterial EPS and the knowledge of heterogeneity of cyanobacterial metabolites.
24299777	7	12	from	aquatilis	1000:1008	arg1	EPS					988:990	EPS	988:990	EPS from S. aquatilis	988:1008	Both EPS and the newly found component FucNAc in EPS from S. aquatilis extend the possible components of cyanobacterial EPS and the knowledge of heterogeneity of cyanobacterial metabolites.
24299777	6	13	theme	N-acetyl-fucosamine	872:890	arg1	amounts					861:867	higher amounts	854:867	higher amounts of N-acetyl-fucosamine (FucNAc)	854:899	Especially remarkable is the composition of these EPS: an arabinofucan with higher amounts of N-acetyl-fucosamine (FucNAc) and only minor quantities of glucose.
24299777	6	13	theme	N-acetyl-fucosamine	872:890	arg1	glucose					930:936	glucose	930:936	glucose	930:936	Especially remarkable is the composition of these EPS: an arabinofucan with higher amounts of N-acetyl-fucosamine (FucNAc) and only minor quantities of glucose.
24299777	6	13	theme	N-acetyl-fucosamine	872:890	arg1	quantities					916:925	only minor quantities	905:925	only minor quantities of glucose	905:936	Especially remarkable is the composition of these EPS: an arabinofucan with higher amounts of N-acetyl-fucosamine (FucNAc) and only minor quantities of glucose.
24299777	6	13	theme	N-acetyl-fucosamine	872:890	arg1	FucNAc					893:898	FucNAc	893:898	FucNAc	893:898	Especially remarkable is the composition of these EPS: an arabinofucan with higher amounts of N-acetyl-fucosamine (FucNAc) and only minor quantities of glucose.
24299777	6	13	theme	N-acetyl-fucosamine	872:890	arg1	N-acetyl-fucosamine					872:890	N-acetyl-fucosamine	872:890	N-acetyl-fucosamine (FucNAc)	872:899	Especially remarkable is the composition of these EPS: an arabinofucan with higher amounts of N-acetyl-fucosamine (FucNAc) and only minor quantities of glucose.
24299777	2	14	theme	cyanobacterial	300:313	arg1	species					315:321	known cyanobacterial species	294:321	known cyanobacterial species	294:321	Considering the high number of known cyanobacterial species the EPS from only a small percentage are investigated in detail.
24299777	4	15	gly	heterogeneity	532:544	arg1	EPS					564:566	cyanobacterial EPS	549:566	cyanobacterial EPS	549:566	The results emphasize the heterogeneity of cyanobacterial EPS.
24299777	5	16	theme	sulfated	703:710	arg1	EPS					712:714	the protein-free but highly sulfated EPS	675:714	acidic proteins as proteoglycan-complexes whereas the protein-free but highly sulfated EPS from S. aquatilis	625:732	S. pevalekii secrets complex hetero-polysaccharides and acidic proteins as proteoglycan-complexes whereas the protein-free but highly sulfated EPS from S. aquatilis only consist of 4 dominant monosaccharides.
24299777	5	17	theme	acidic	625:630	arg1	proteins					632:639	acidic proteins	625:639	acidic proteins as proteoglycan-complexes whereas the protein-free but highly sulfated EPS from S. aquatilis	625:732	S. pevalekii secrets complex hetero-polysaccharides and acidic proteins as proteoglycan-complexes whereas the protein-free but highly sulfated EPS from S. aquatilis only consist of 4 dominant monosaccharides.
24299777	2	18	theme	known	294:298	arg1	species					315:321	known cyanobacterial species	294:321	known cyanobacterial species	294:321	Considering the high number of known cyanobacterial species the EPS from only a small percentage are investigated in detail.
24299777	5	19	dep	secrets	582:588	arg1	hetero-polysaccharides					598:619	complex hetero-polysaccharides	590:619	complex hetero-polysaccharides	590:619	S. pevalekii secrets complex hetero-polysaccharides and acidic proteins as proteoglycan-complexes whereas the protein-free but highly sulfated EPS from S. aquatilis only consist of 4 dominant monosaccharides.
24299777	5	19	dep	secrets	582:588	arg1	proteins					632:639	acidic proteins	625:639	acidic proteins as proteoglycan-complexes whereas the protein-free but highly sulfated EPS from S. aquatilis	625:732	S. pevalekii secrets complex hetero-polysaccharides and acidic proteins as proteoglycan-complexes whereas the protein-free but highly sulfated EPS from S. aquatilis only consist of 4 dominant monosaccharides.
24299777	4	20	theme	cyanobacterial	549:562	arg1	EPS					564:566	cyanobacterial EPS	549:566	cyanobacterial EPS	549:566	The results emphasize the heterogeneity of cyanobacterial EPS.
24299777	6	21	theme	higher	854:859	arg1	amounts					861:867	higher amounts	854:867	higher amounts of N-acetyl-fucosamine (FucNAc)	854:899	Especially remarkable is the composition of these EPS: an arabinofucan with higher amounts of N-acetyl-fucosamine (FucNAc) and only minor quantities of glucose.
24299777	6	21	theme	higher	854:859	arg1	glucose					930:936	glucose	930:936	glucose	930:936	Especially remarkable is the composition of these EPS: an arabinofucan with higher amounts of N-acetyl-fucosamine (FucNAc) and only minor quantities of glucose.
24299777	6	21	theme	higher	854:859	arg1	FucNAc					893:898	FucNAc	893:898	FucNAc	893:898	Especially remarkable is the composition of these EPS: an arabinofucan with higher amounts of N-acetyl-fucosamine (FucNAc) and only minor quantities of glucose.
24299777	6	21	theme	higher	854:859	arg1	N-acetyl-fucosamine					872:890	N-acetyl-fucosamine	872:890	N-acetyl-fucosamine (FucNAc)	872:899	Especially remarkable is the composition of these EPS: an arabinofucan with higher amounts of N-acetyl-fucosamine (FucNAc) and only minor quantities of glucose.
24299777	3	22	theme	aquatilis	457:465	arg1	strains					432:438	the unicellular strains	416:438	the unicellular strains of Synechocystis aquatilis and S. pevalekii with various methods	416:503	This work examined EPS from the unicellular strains of Synechocystis aquatilis and S. pevalekii with various methods.
24299777	0	23	theme	aquatilis	36:44	arg1	Exopolysaccharides					0:17	Exopolysaccharides	0:17	Exopolysaccharides of Synechocystis aquatilis	0:44	Exopolysaccharides of Synechocystis aquatilis are sulfated arabinofucans containing N-acetyl-fucosamine.
24299777	0	23	theme	aquatilis	36:44	arg1	arabinofucans					59:71	sulfated arabinofucans	50:71	sulfated arabinofucans containing N-acetyl-fucosamine	50:102	Exopolysaccharides of Synechocystis aquatilis are sulfated arabinofucans containing N-acetyl-fucosamine.
24299777	7	24	from	FucNAc	978:983	arg1	EPS					988:990	EPS	988:990	EPS from S. aquatilis	988:1008	Both EPS and the newly found component FucNAc in EPS from S. aquatilis extend the possible components of cyanobacterial EPS and the knowledge of heterogeneity of cyanobacterial metabolites.
24299777	7	24	from	FucNAc	978:983	arg1	aquatilis					1000:1008	S. aquatilis	997:1008	S. aquatilis	997:1008	Both EPS and the newly found component FucNAc in EPS from S. aquatilis extend the possible components of cyanobacterial EPS and the knowledge of heterogeneity of cyanobacterial metabolites.
24299777	7	25	theme	possible	1021:1028	arg1	components					1030:1039	the possible components	1017:1039	the possible components of cyanobacterial EPS	1017:1061	Both EPS and the newly found component FucNAc in EPS from S. aquatilis extend the possible components of cyanobacterial EPS and the knowledge of heterogeneity of cyanobacterial metabolites.
24299777	3	26	theme	various	489:495	arg1	methods					497:503	various methods	489:503	various methods	489:503	This work examined EPS from the unicellular strains of Synechocystis aquatilis and S. pevalekii with various methods.
24299777	0	27	theme	Synechocystis	22:34	arg1	aquatilis					36:44	Synechocystis aquatilis	22:44	Synechocystis aquatilis	22:44	Exopolysaccharides of Synechocystis aquatilis are sulfated arabinofucans containing N-acetyl-fucosamine.
24299777	3	28	with	strains	432:438	arg1	methods					497:503	various methods	489:503	various methods	489:503	This work examined EPS from the unicellular strains of Synechocystis aquatilis and S. pevalekii with various methods.
24299777	1	29	theme	excreted	228:235	arg1	EPS					257:259	EPS	257:259	EPS	257:259	Cyanobacteria are known to be a rather diverse group of organisms regarding e.g. morphology, metabolism and composition of excreted exopolysaccharides (EPS).
24299777	1	29	theme	excreted	228:235	arg1	exopolysaccharides					237:254	excreted exopolysaccharides	228:254	excreted exopolysaccharides (EPS)	228:260	Cyanobacteria are known to be a rather diverse group of organisms regarding e.g. morphology, metabolism and composition of excreted exopolysaccharides (EPS).
24299777	0	30	theme	sulfated	50:57	arg1	Exopolysaccharides					0:17	Exopolysaccharides	0:17	Exopolysaccharides of Synechocystis aquatilis	0:44	Exopolysaccharides of Synechocystis aquatilis are sulfated arabinofucans containing N-acetyl-fucosamine.
24299777	0	30	theme	sulfated	50:57	arg1	arabinofucans					59:71	sulfated arabinofucans	50:71	sulfated arabinofucans containing N-acetyl-fucosamine	50:102	Exopolysaccharides of Synechocystis aquatilis are sulfated arabinofucans containing N-acetyl-fucosamine.
24299777	1	31	theme	exopolysaccharides	237:254	arg1	composition					213:223	composition	213:223	composition	213:223	Cyanobacteria are known to be a rather diverse group of organisms regarding e.g. morphology, metabolism and composition of excreted exopolysaccharides (EPS).
24299777	1	31	theme	exopolysaccharides	237:254	arg1	metabolism					198:207	metabolism	198:207	metabolism	198:207	Cyanobacteria are known to be a rather diverse group of organisms regarding e.g. morphology, metabolism and composition of excreted exopolysaccharides (EPS).
24299777	1	31	theme	exopolysaccharides	237:254	arg1	morphology					186:195	e.g. morphology	181:195	e.g. morphology	181:195	Cyanobacteria are known to be a rather diverse group of organisms regarding e.g. morphology, metabolism and composition of excreted exopolysaccharides (EPS).
24299777	3	32	theme	pevalekii	474:482	arg1	strains					432:438	the unicellular strains	416:438	the unicellular strains of Synechocystis aquatilis and S. pevalekii with various methods	416:503	This work examined EPS from the unicellular strains of Synechocystis aquatilis and S. pevalekii with various methods.
24299777	7	33	gly	heterogeneity	1084:1096	arg1	metabolites					1116:1126	cyanobacterial metabolites	1101:1126	cyanobacterial metabolites	1101:1126	Both EPS and the newly found component FucNAc in EPS from S. aquatilis extend the possible components of cyanobacterial EPS and the knowledge of heterogeneity of cyanobacterial metabolites.
24299777	6	34	theme	glucose	930:936	arg1	amounts					861:867	higher amounts	854:867	higher amounts of N-acetyl-fucosamine (FucNAc)	854:899	Especially remarkable is the composition of these EPS: an arabinofucan with higher amounts of N-acetyl-fucosamine (FucNAc) and only minor quantities of glucose.
24299777	6	34	theme	glucose	930:936	arg1	glucose					930:936	glucose	930:936	glucose	930:936	Especially remarkable is the composition of these EPS: an arabinofucan with higher amounts of N-acetyl-fucosamine (FucNAc) and only minor quantities of glucose.
24299777	6	34	theme	glucose	930:936	arg1	quantities					916:925	only minor quantities	905:925	only minor quantities of glucose	905:936	Especially remarkable is the composition of these EPS: an arabinofucan with higher amounts of N-acetyl-fucosamine (FucNAc) and only minor quantities of glucose.
24299777	6	34	theme	glucose	930:936	arg1	FucNAc					893:898	FucNAc	893:898	FucNAc	893:898	Especially remarkable is the composition of these EPS: an arabinofucan with higher amounts of N-acetyl-fucosamine (FucNAc) and only minor quantities of glucose.
24299777	6	34	theme	glucose	930:936	arg1	N-acetyl-fucosamine					872:890	N-acetyl-fucosamine	872:890	N-acetyl-fucosamine (FucNAc)	872:899	Especially remarkable is the composition of these EPS: an arabinofucan with higher amounts of N-acetyl-fucosamine (FucNAc) and only minor quantities of glucose.
24299777	4	35	theme	EPS	564:566	arg1	heterogeneity					532:544	the heterogeneity	528:544	the heterogeneity of cyanobacterial EPS	528:566	The results emphasize the heterogeneity of cyanobacterial EPS.
24299777	7	36	theme	metabolites	1116:1126	arg1	heterogeneity					1084:1096	heterogeneity	1084:1096	heterogeneity of cyanobacterial metabolites	1084:1126	Both EPS and the newly found component FucNAc in EPS from S. aquatilis extend the possible components of cyanobacterial EPS and the knowledge of heterogeneity of cyanobacterial metabolites.
24299777	5	37	theme	complex	590:596	arg1	hetero-polysaccharides					598:619	complex hetero-polysaccharides	590:619	complex hetero-polysaccharides	590:619	S. pevalekii secrets complex hetero-polysaccharides and acidic proteins as proteoglycan-complexes whereas the protein-free but highly sulfated EPS from S. aquatilis only consist of 4 dominant monosaccharides.
24299777	3	38	theme	unicellular	420:430	arg1	strains					432:438	the unicellular strains	416:438	the unicellular strains of Synechocystis aquatilis and S. pevalekii with various methods	416:503	This work examined EPS from the unicellular strains of Synechocystis aquatilis and S. pevalekii with various methods.
24299777	1	39	theme	diverse	144:150	arg1	group					152:156	a rather diverse group	135:156	a rather diverse group of organisms regarding e.g. morphology, metabolism and composition of excreted exopolysaccharides (EPS)	135:260	Cyanobacteria are known to be a rather diverse group of organisms regarding e.g. morphology, metabolism and composition of excreted exopolysaccharides (EPS).
24299777	1	39	theme	diverse	144:150	arg1	Cyanobacteria					105:117	Cyanobacteria	105:117	Cyanobacteria	105:117	Cyanobacteria are known to be a rather diverse group of organisms regarding e.g. morphology, metabolism and composition of excreted exopolysaccharides (EPS).
24299777	1	39	theme	diverse	144:150	arg1	organisms					161:169	organisms	161:169	organisms regarding e.g. morphology, metabolism and composition of excreted exopolysaccharides (EPS)	161:260	Cyanobacteria are known to be a rather diverse group of organisms regarding e.g. morphology, metabolism and composition of excreted exopolysaccharides (EPS).
24299777	5	40	from	aquatilis	724:732	arg1	EPS					712:714	the protein-free but highly sulfated EPS	675:714	acidic proteins as proteoglycan-complexes whereas the protein-free but highly sulfated EPS from S. aquatilis	625:732	S. pevalekii secrets complex hetero-polysaccharides and acidic proteins as proteoglycan-complexes whereas the protein-free but highly sulfated EPS from S. aquatilis only consist of 4 dominant monosaccharides.
24299777	5	40	from	aquatilis	724:732	arg1	hetero-polysaccharides					598:619	complex hetero-polysaccharides	590:619	complex hetero-polysaccharides	590:619	S. pevalekii secrets complex hetero-polysaccharides and acidic proteins as proteoglycan-complexes whereas the protein-free but highly sulfated EPS from S. aquatilis only consist of 4 dominant monosaccharides.
24299777	5	40	from	aquatilis	724:732	arg1	proteins					632:639	acidic proteins	625:639	acidic proteins as proteoglycan-complexes whereas the protein-free but highly sulfated EPS from S. aquatilis	625:732	S. pevalekii secrets complex hetero-polysaccharides and acidic proteins as proteoglycan-complexes whereas the protein-free but highly sulfated EPS from S. aquatilis only consist of 4 dominant monosaccharides.
24299777	5	41	theme	dominant	752:759	arg1	monosaccharides					761:775	4 dominant monosaccharides	750:775	4 dominant monosaccharides	750:775	S. pevalekii secrets complex hetero-polysaccharides and acidic proteins as proteoglycan-complexes whereas the protein-free but highly sulfated EPS from S. aquatilis only consist of 4 dominant monosaccharides.
24299777	6	42	theme	minor	910:914	arg1	quantities					916:925	only minor quantities	905:925	only minor quantities of glucose	905:936	Especially remarkable is the composition of these EPS: an arabinofucan with higher amounts of N-acetyl-fucosamine (FucNAc) and only minor quantities of glucose.
24299777	6	43	theme	EPS	828:830	arg1	remarkable					789:798	remarkable	789:798	remarkable	789:798	Especially remarkable is the composition of these EPS: an arabinofucan with higher amounts of N-acetyl-fucosamine (FucNAc) and only minor quantities of glucose.
24299777	6	43	theme	EPS	828:830	arg1	composition					807:817	the composition	803:817	the composition of these EPS	803:830	Especially remarkable is the composition of these EPS: an arabinofucan with higher amounts of N-acetyl-fucosamine (FucNAc) and only minor quantities of glucose.
24299777	7	44	theme	cyanobacterial	1101:1114	arg1	metabolites					1116:1126	cyanobacterial metabolites	1101:1126	cyanobacterial metabolites	1101:1126	Both EPS and the newly found component FucNAc in EPS from S. aquatilis extend the possible components of cyanobacterial EPS and the knowledge of heterogeneity of cyanobacterial metabolites.
24299777	7	45	theme	EPS	1059:1061	arg1	knowledge					1071:1079	the knowledge	1067:1079	the knowledge of heterogeneity of cyanobacterial metabolites	1067:1126	Both EPS and the newly found component FucNAc in EPS from S. aquatilis extend the possible components of cyanobacterial EPS and the knowledge of heterogeneity of cyanobacterial metabolites.
24299777	7	45	theme	EPS	1059:1061	arg1	components					1030:1039	the possible components	1017:1039	the possible components of cyanobacterial EPS	1017:1061	Both EPS and the newly found component FucNAc in EPS from S. aquatilis extend the possible components of cyanobacterial EPS and the knowledge of heterogeneity of cyanobacterial metabolites.
24299777	2	46	theme	small	343:347	arg1	percentage					349:358	only a small percentage	336:358	only a small percentage	336:358	Considering the high number of known cyanobacterial species the EPS from only a small percentage are investigated in detail.
24299777	7	47	theme	found	962:966	arg1	FucNAc					978:983	the newly found component FucNAc	952:983	the newly found component FucNAc in EPS from S. aquatilis	952:1008	Both EPS and the newly found component FucNAc in EPS from S. aquatilis extend the possible components of cyanobacterial EPS and the knowledge of heterogeneity of cyanobacterial metabolites.
24299777	2	48	theme	high	279:282	arg1	number					284:289	the high number	275:289	the high number of known cyanobacterial species	275:321	Considering the high number of known cyanobacterial species the EPS from only a small percentage are investigated in detail.
24299777	1	49	theme	organisms	161:169	arg1	group					152:156	a rather diverse group	135:156	a rather diverse group of organisms regarding e.g. morphology, metabolism and composition of excreted exopolysaccharides (EPS)	135:260	Cyanobacteria are known to be a rather diverse group of organisms regarding e.g. morphology, metabolism and composition of excreted exopolysaccharides (EPS).
24299777	1	49	theme	organisms	161:169	arg1	Cyanobacteria					105:117	Cyanobacteria	105:117	Cyanobacteria	105:117	Cyanobacteria are known to be a rather diverse group of organisms regarding e.g. morphology, metabolism and composition of excreted exopolysaccharides (EPS).
24299777	1	49	theme	organisms	161:169	arg1	organisms					161:169	organisms	161:169	organisms regarding e.g. morphology, metabolism and composition of excreted exopolysaccharides (EPS)	161:260	Cyanobacteria are known to be a rather diverse group of organisms regarding e.g. morphology, metabolism and composition of excreted exopolysaccharides (EPS).
24299777	7	50	theme	component	968:976	arg1	FucNAc					978:983	the newly found component FucNAc	952:983	the newly found component FucNAc in EPS from S. aquatilis	952:1008	Both EPS and the newly found component FucNAc in EPS from S. aquatilis extend the possible components of cyanobacterial EPS and the knowledge of heterogeneity of cyanobacterial metabolites.
24299777	5	51	theme	S.	569:570	arg1	secrets					582:588	S. pevalekii secrets complex hetero-polysaccharides and acidic proteins as proteoglycan-complexes whereas the protein-free but highly sulfated EPS from S. aquatilis only	569:737	S. pevalekii secrets complex hetero-polysaccharides and acidic proteins as proteoglycan-complexes whereas the protein-free but highly sulfated EPS from S. aquatilis only	569:737	S. pevalekii secrets complex hetero-polysaccharides and acidic proteins as proteoglycan-complexes whereas the protein-free but highly sulfated EPS from S. aquatilis only consist of 4 dominant monosaccharides.
26369027	8	0	theme	implant	1053:1059	arg1	surface					1024:1030	the surface	1020:1030	the surface of the MH/CTS coated implant	1020:1059	The in vitro formation of bonelike apatite layer on the surface of the MH/CTS coated implant was observed from simulated body fluid studies.
26369027	6	1	theme	coated	829:834	arg1	implant					836:842	MH/chitosan (CTS) coated implant	811:842	MH/chitosan (CTS) coated implant	811:842	Surface morphology of MH/chitosan (CTS) coated implant was found to be uniform without any defects.
26369027	4	2	theme	Mg	531:532	arg1	incorporation					514:526	incorporation	514:526	incorporation of Mg in HAp lattice	514:547	On incorporation of Mg in HAp lattice, an increase of 20-66 nm in specific surface area was observed in microporous HAp particles.
26369027	1	3	theme	mol	243:245	arg1	%					246:246	0, 1, 2, and 3 mol%	228:246	0, 1, 2, and 3 mol%	228:246	In this investigation, ultrasonication process was used for the synthesis of magnesium doped nano-hydroxyapatite (MH) (0, 1, 2, and 3 mol% of Mg concentration) particles with controlled size and surface morphology.
26369027	1	3	theme	mol	243:245	arg1	concentration					254:266	Mg concentration	251:266	Mg concentration	251:266	In this investigation, ultrasonication process was used for the synthesis of magnesium doped nano-hydroxyapatite (MH) (0, 1, 2, and 3 mol% of Mg concentration) particles with controlled size and surface morphology.
26369027	8	4	theme	simulated	1079:1087	arg1	studies					1100:1106	simulated body fluid studies	1079:1106	simulated body fluid studies	1079:1106	The in vitro formation of bonelike apatite layer on the surface of the MH/CTS coated implant was observed from simulated body fluid studies.
26369027	1	5	with	particles	269:277	arg1	size					295:298	controlled size	284:298	controlled size	284:298	In this investigation, ultrasonication process was used for the synthesis of magnesium doped nano-hydroxyapatite (MH) (0, 1, 2, and 3 mol% of Mg concentration) particles with controlled size and surface morphology.
26369027	1	5	with	particles	269:277	arg1	morphology					312:321	surface morphology	304:321	surface morphology	304:321	In this investigation, ultrasonication process was used for the synthesis of magnesium doped nano-hydroxyapatite (MH) (0, 1, 2, and 3 mol% of Mg concentration) particles with controlled size and surface morphology.
26369027	11	6	theme	incorporated	1530:1541	arg1	composite					1551:1559	the Mg incorporated HAp/CTS composite	1523:1559	the Mg incorporated HAp/CTS composite	1523:1559	From the cytotoxicity analysis against NIH 3T3 fibroblast, it was observed that the Mg incorporated HAp/CTS composite was less toxic than the MHO/CTS composite.
26369027	11	6	theme	incorporated	1530:1541	arg1	toxic					1570:1574	toxic	1570:1574	toxic	1570:1574	From the cytotoxicity analysis against NIH 3T3 fibroblast, it was observed that the Mg incorporated HAp/CTS composite was less toxic than the MHO/CTS composite.
26369027	1	7	theme	doped	196:200	arg1	nano-hydroxyapatite					202:220	magnesium doped nano-hydroxyapatite	186:220	magnesium doped nano-hydroxyapatite (MH) (0, 1, 2, and 3 mol% of Mg concentration) particles with controlled size and surface morphology	186:321	In this investigation, ultrasonication process was used for the synthesis of magnesium doped nano-hydroxyapatite (MH) (0, 1, 2, and 3 mol% of Mg concentration) particles with controlled size and surface morphology.
26369027	1	7	theme	doped	196:200	arg1	MH					223:224	MH	223:224	MH	223:224	In this investigation, ultrasonication process was used for the synthesis of magnesium doped nano-hydroxyapatite (MH) (0, 1, 2, and 3 mol% of Mg concentration) particles with controlled size and surface morphology.
26369027	8	8	theme	fluid	1094:1098	arg1	studies					1100:1106	simulated body fluid studies	1079:1106	simulated body fluid studies	1079:1106	The in vitro formation of bonelike apatite layer on the surface of the MH/CTS coated implant was observed from simulated body fluid studies.
26369027	12	9	theme	excellent	1692:1700	arg1	implant					1677:1683	the MH/CTS nanocomposites coated implant	1644:1683	the MH/CTS nanocomposites coated implant	1644:1683	From this result, it was concluded that the MH/CTS nanocomposites coated implant is the excellent material for implants.
26369027	12	9	theme	excellent	1692:1700	arg1	material					1702:1709	the excellent material	1688:1709	the excellent material for implants	1688:1722	From this result, it was concluded that the MH/CTS nanocomposites coated implant is the excellent material for implants.
26369027	1	10	used	used	160:163	arg2	process					148:154	ultrasonication process	132:154	ultrasonication process	132:154	In this investigation, ultrasonication process was used for the synthesis of magnesium doped nano-hydroxyapatite (MH) (0, 1, 2, and 3 mol% of Mg concentration) particles with controlled size and surface morphology.
26369027	4	11	theme	surface	586:592	arg1	area					594:597	specific surface area	577:597	specific surface area	577:597	On incorporation of Mg in HAp lattice, an increase of 20-66 nm in specific surface area was observed in microporous HAp particles.
26369027	4	12	from	increase	553:560	arg1	area					594:597	specific surface area	577:597	specific surface area	577:597	On incorporation of Mg in HAp lattice, an increase of 20-66 nm in specific surface area was observed in microporous HAp particles.
26369027	9	13	theme	composites	1150:1159	arg1	activity					1127:1134	The antimicrobial activity	1109:1134	The antimicrobial activity of the MH/CTS composites against gram-positive and gram-negative bacterial strains	1109:1217	The antimicrobial activity of the MH/CTS composites against gram-positive and gram-negative bacterial strains indicated that increasing Mg concentration enhanced antimicrobial properties.
26369027	4	14	theme	HAp	627:629	arg1	particles					631:639	microporous HAp particles	615:639	microporous HAp particles	615:639	On incorporation of Mg in HAp lattice, an increase of 20-66 nm in specific surface area was observed in microporous HAp particles.
26369027	9	15	theme	gram-positive	1169:1181	arg1	strains					1211:1217	gram-positive and gram-negative bacterial strains	1169:1217	gram-positive and gram-negative bacterial strains	1169:1217	The antimicrobial activity of the MH/CTS composites against gram-positive and gram-negative bacterial strains indicated that increasing Mg concentration enhanced antimicrobial properties.
26369027	7	16	theme	implant	915:921	arg1	rate					903:906	The corrosion rate	889:906	The corrosion rate of the implant	889:921	The corrosion rate of the implant decreased with increase in Mg concentration.
26369027	7	17	theme	Mg	950:951	arg1	concentration					953:965	Mg concentration	950:965	Mg concentration	950:965	The corrosion rate of the implant decreased with increase in Mg concentration.
26369027	9	18	theme	gram-negative	1187:1199	arg1	strains					1211:1217	gram-positive and gram-negative bacterial strains	1169:1217	gram-positive and gram-negative bacterial strains	1169:1217	The antimicrobial activity of the MH/CTS composites against gram-positive and gram-negative bacterial strains indicated that increasing Mg concentration enhanced antimicrobial properties.
26369027	5	19	contain	possess	689:695	arg2	composition					712:722	stoichiometric composition	697:722	stoichiometric composition	697:722	XRF and XRD patterns reveal that the particles possess stoichiometric composition with reduced crystallinity with respect to the Mg concentration.
26369027	5	19	contain	possess	689:695	arg1	particles					679:687	the particles	675:687	the particles	675:687	XRF and XRD patterns reveal that the particles possess stoichiometric composition with reduced crystallinity with respect to the Mg concentration.
26369027	11	20	theme	cytotoxicity	1452:1463	arg1	analysis					1465:1472	the cytotoxicity analysis	1448:1472	the cytotoxicity analysis against NIH 3T3 fibroblast	1448:1499	From the cytotoxicity analysis against NIH 3T3 fibroblast, it was observed that the Mg incorporated HAp/CTS composite was less toxic than the MHO/CTS composite.
26369027	7	21	theme	corrosion	893:901	arg1	rate					903:906	The corrosion rate	889:906	The corrosion rate of the implant	889:921	The corrosion rate of the implant decreased with increase in Mg concentration.
26369027	3	22	dep	40	504:505	arg1	to					501:502	to	501:502	to	501:502	Increase in the concentration of Mg reduced the particle size distribution from 60 to 40 nm.
26369027	8	23	theme	apatite	1003:1009	arg1	layer					1011:1015	bonelike apatite layer	994:1015	bonelike apatite layer	994:1015	The in vitro formation of bonelike apatite layer on the surface of the MH/CTS coated implant was observed from simulated body fluid studies.
26369027	5	24	theme	XRD	650:652	arg1	patterns					654:661	XRF and XRD patterns	642:661	XRF and XRD patterns	642:661	XRF and XRD patterns reveal that the particles possess stoichiometric composition with reduced crystallinity with respect to the Mg concentration.
26369027	10	25	theme	magnesium	1425:1433	arg1	concentration					1408:1420	the concentration	1404:1420	the concentration of magnesium in HAp	1404:1440	Nanoindentation analysis of apatite coated implant surface reveals that the mechanical property depends on the concentration of magnesium in HAp.
26369027	1	26	theme	surface	304:310	arg1	morphology					312:321	surface morphology	304:321	surface morphology	304:321	In this investigation, ultrasonication process was used for the synthesis of magnesium doped nano-hydroxyapatite (MH) (0, 1, 2, and 3 mol% of Mg concentration) particles with controlled size and surface morphology.
26369027	12	27	theme	nanocomposites	1655:1668	arg1	implant					1677:1683	the MH/CTS nanocomposites coated implant	1644:1683	the MH/CTS nanocomposites coated implant	1644:1683	From this result, it was concluded that the MH/CTS nanocomposites coated implant is the excellent material for implants.
26369027	12	27	theme	nanocomposites	1655:1668	arg1	material					1702:1709	the excellent material	1688:1709	the excellent material for implants	1688:1722	From this result, it was concluded that the MH/CTS nanocomposites coated implant is the excellent material for implants.
26369027	11	28	theme	3T3	1486:1488	arg1	fibroblast					1490:1499	NIH 3T3 fibroblast	1482:1499	NIH 3T3 fibroblast	1482:1499	From the cytotoxicity analysis against NIH 3T3 fibroblast, it was observed that the Mg incorporated HAp/CTS composite was less toxic than the MHO/CTS composite.
26369027	9	29	theme	Mg	1245:1246	arg1	concentration					1248:1260	increasing Mg concentration	1234:1260	increasing Mg concentration	1234:1260	The antimicrobial activity of the MH/CTS composites against gram-positive and gram-negative bacterial strains indicated that increasing Mg concentration enhanced antimicrobial properties.
26369027	2	30	theme	particles	352:360	arg1	size					328:331	The size	324:331	The size of the prepared MH particles	324:360	The size of the prepared MH particles was in the range of 20-100 nm with narrow distribution.
26369027	2	31	theme	prepared	340:347	arg1	particles					352:360	the prepared MH particles	336:360	the prepared MH particles	336:360	The size of the prepared MH particles was in the range of 20-100 nm with narrow distribution.
26369027	0	32	theme	Mg-Doped	0:7	arg1	Hydroxyapatite/Chitosan					9:31	Mg-Doped Hydroxyapatite/Chitosan	0:31	Mg-Doped Hydroxyapatite/Chitosan	0:31	Mg-Doped Hydroxyapatite/Chitosan Composite Coated 316L Stainless Steel Implants for Biomedical Applications.
26369027	1	33	theme	Mg	251:252	arg1	concentration					254:266	Mg concentration	251:266	Mg concentration	251:266	In this investigation, ultrasonication process was used for the synthesis of magnesium doped nano-hydroxyapatite (MH) (0, 1, 2, and 3 mol% of Mg concentration) particles with controlled size and surface morphology.
26369027	1	34	dep	nano-hydroxyapatite	202:220	arg1	%					246:246	0, 1, 2, and 3 mol%	228:246	0, 1, 2, and 3 mol%	228:246	In this investigation, ultrasonication process was used for the synthesis of magnesium doped nano-hydroxyapatite (MH) (0, 1, 2, and 3 mol% of Mg concentration) particles with controlled size and surface morphology.
26369027	1	34	dep	nano-hydroxyapatite	202:220	arg1	concentration					254:266	Mg concentration	251:266	Mg concentration	251:266	In this investigation, ultrasonication process was used for the synthesis of magnesium doped nano-hydroxyapatite (MH) (0, 1, 2, and 3 mol% of Mg concentration) particles with controlled size and surface morphology.
26369027	0	35	theme	316L	50:53	arg1	Implants					71:78	Coated 316L Stainless Steel Implants	43:78	Coated 316L Stainless Steel Implants	43:78	Mg-Doped Hydroxyapatite/Chitosan Composite Coated 316L Stainless Steel Implants for Biomedical Applications.
26369027	5	36	theme	stoichiometric	697:710	arg1	composition					712:722	stoichiometric composition	697:722	stoichiometric composition	697:722	XRF and XRD patterns reveal that the particles possess stoichiometric composition with reduced crystallinity with respect to the Mg concentration.
26369027	6	37	theme	Surface	789:795	arg1	morphology					797:806	Surface morphology	789:806	Surface morphology of MH/chitosan (CTS) coated implant	789:842	Surface morphology of MH/chitosan (CTS) coated implant was found to be uniform without any defects.
26369027	10	38	theme	Nanoindentation	1297:1311	arg1	analysis					1313:1320	Nanoindentation analysis	1297:1320	Nanoindentation analysis of apatite coated implant surface	1297:1354	Nanoindentation analysis of apatite coated implant surface reveals that the mechanical property depends on the concentration of magnesium in HAp.
26369027	0	39	theme	Steel	65:69	arg1	Implants					71:78	Coated 316L Stainless Steel Implants	43:78	Coated 316L Stainless Steel Implants	43:78	Mg-Doped Hydroxyapatite/Chitosan Composite Coated 316L Stainless Steel Implants for Biomedical Applications.
26369027	3	40	from	Increase	418:425	arg1	concentration					434:446	the concentration	430:446	the concentration of Mg	430:452	Increase in the concentration of Mg reduced the particle size distribution from 60 to 40 nm.
26369027	10	41	theme	coated	1333:1338	arg1	surface					1348:1354	apatite coated implant surface	1325:1354	apatite coated implant surface	1325:1354	Nanoindentation analysis of apatite coated implant surface reveals that the mechanical property depends on the concentration of magnesium in HAp.
26369027	8	42	dep	in	972:973	arg1	vitro					975:979	vitro	975:979	vitro	975:979	The in vitro formation of bonelike apatite layer on the surface of the MH/CTS coated implant was observed from simulated body fluid studies.
26369027	3	43	theme	Mg	451:452	arg1	concentration					434:446	the concentration	430:446	the concentration of Mg	430:452	Increase in the concentration of Mg reduced the particle size distribution from 60 to 40 nm.
26369027	8	44	theme	coated	1046:1051	arg1	implant					1053:1059	the MH/CTS coated implant	1035:1059	the MH/CTS coated implant	1035:1059	The in vitro formation of bonelike apatite layer on the surface of the MH/CTS coated implant was observed from simulated body fluid studies.
26369027	6	45	theme	implant	836:842	arg1	morphology					797:806	Surface morphology	789:806	Surface morphology of MH/chitosan (CTS) coated implant	789:842	Surface morphology of MH/chitosan (CTS) coated implant was found to be uniform without any defects.
26369027	11	46	dep	incorporated	1530:1541	arg1	Mg					1527:1528	Mg	1527:1528	Mg	1527:1528	From the cytotoxicity analysis against NIH 3T3 fibroblast, it was observed that the Mg incorporated HAp/CTS composite was less toxic than the MHO/CTS composite.
26369027	11	47	theme	HAp/CTS	1543:1549	arg1	composite					1551:1559	the Mg incorporated HAp/CTS composite	1523:1559	the Mg incorporated HAp/CTS composite	1523:1559	From the cytotoxicity analysis against NIH 3T3 fibroblast, it was observed that the Mg incorporated HAp/CTS composite was less toxic than the MHO/CTS composite.
26369027	11	47	theme	HAp/CTS	1543:1549	arg1	toxic					1570:1574	toxic	1570:1574	toxic	1570:1574	From the cytotoxicity analysis against NIH 3T3 fibroblast, it was observed that the Mg incorporated HAp/CTS composite was less toxic than the MHO/CTS composite.
26369027	10	48	theme	surface	1348:1354	arg1	analysis					1313:1320	Nanoindentation analysis	1297:1320	Nanoindentation analysis of apatite coated implant surface	1297:1354	Nanoindentation analysis of apatite coated implant surface reveals that the mechanical property depends on the concentration of magnesium in HAp.
26369027	3	49	theme	size	475:478	arg1	distribution					480:491	the particle size distribution	462:491	the particle size distribution from 60 to 40 nm	462:508	Increase in the concentration of Mg reduced the particle size distribution from 60 to 40 nm.
26369027	1	50	theme	magnesium	186:194	arg1	nano-hydroxyapatite					202:220	magnesium doped nano-hydroxyapatite	186:220	magnesium doped nano-hydroxyapatite (MH) (0, 1, 2, and 3 mol% of Mg concentration) particles with controlled size and surface morphology	186:321	In this investigation, ultrasonication process was used for the synthesis of magnesium doped nano-hydroxyapatite (MH) (0, 1, 2, and 3 mol% of Mg concentration) particles with controlled size and surface morphology.
26369027	1	50	theme	magnesium	186:194	arg1	MH					223:224	MH	223:224	MH	223:224	In this investigation, ultrasonication process was used for the synthesis of magnesium doped nano-hydroxyapatite (MH) (0, 1, 2, and 3 mol% of Mg concentration) particles with controlled size and surface morphology.
26369027	2	51	with	range	373:377	arg1	distribution					404:415	narrow distribution	397:415	narrow distribution	397:415	The size of the prepared MH particles was in the range of 20-100 nm with narrow distribution.
26369027	4	52	theme	nm	571:572	arg1	increase					553:560	an increase	550:560	an increase of 20-66 nm in specific surface area	550:597	On incorporation of Mg in HAp lattice, an increase of 20-66 nm in specific surface area was observed in microporous HAp particles.
26369027	9	53	theme	antimicrobial	1113:1125	arg1	activity					1127:1134	The antimicrobial activity	1109:1134	The antimicrobial activity of the MH/CTS composites against gram-positive and gram-negative bacterial strains	1109:1217	The antimicrobial activity of the MH/CTS composites against gram-positive and gram-negative bacterial strains indicated that increasing Mg concentration enhanced antimicrobial properties.
26369027	1	54	theme	nano-hydroxyapatite	202:220	arg1	particles					269:277	magnesium doped nano-hydroxyapatite (MH) (0, 1, 2, and 3 mol% of Mg concentration) particles	186:277	magnesium doped nano-hydroxyapatite (MH) (0, 1, 2, and 3 mol% of Mg concentration) particles with controlled size and surface morphology	186:321	In this investigation, ultrasonication process was used for the synthesis of magnesium doped nano-hydroxyapatite (MH) (0, 1, 2, and 3 mol% of Mg concentration) particles with controlled size and surface morphology.
26369027	8	55	theme	body	1089:1092	arg1	studies					1100:1106	simulated body fluid studies	1079:1106	simulated body fluid studies	1079:1106	The in vitro formation of bonelike apatite layer on the surface of the MH/CTS coated implant was observed from simulated body fluid studies.
26369027	10	56	from	concentration	1408:1420	arg1	HAp					1438:1440	HAp	1438:1440	HAp	1438:1440	Nanoindentation analysis of apatite coated implant surface reveals that the mechanical property depends on the concentration of magnesium in HAp.
26369027	4	57	theme	specific	577:584	arg1	area					594:597	specific surface area	577:597	specific surface area	577:597	On incorporation of Mg in HAp lattice, an increase of 20-66 nm in specific surface area was observed in microporous HAp particles.
26369027	10	58	theme	mechanical	1373:1382	arg1	property					1384:1391	the mechanical property	1369:1391	the mechanical property	1369:1391	Nanoindentation analysis of apatite coated implant surface reveals that the mechanical property depends on the concentration of magnesium in HAp.
26369027	9	59	theme	MH/CTS	1143:1148	arg1	composites					1150:1159	the MH/CTS composites	1139:1159	the MH/CTS composites	1139:1159	The antimicrobial activity of the MH/CTS composites against gram-positive and gram-negative bacterial strains indicated that increasing Mg concentration enhanced antimicrobial properties.
26369027	5	60	theme	Mg	771:772	arg1	concentration					774:786	the Mg concentration	767:786	the Mg concentration	767:786	XRF and XRD patterns reveal that the particles possess stoichiometric composition with reduced crystallinity with respect to the Mg concentration.
26369027	8	61	from	formation	981:989	arg1	surface					1024:1030	the surface	1020:1030	the surface of the MH/CTS coated implant	1020:1059	The in vitro formation of bonelike apatite layer on the surface of the MH/CTS coated implant was observed from simulated body fluid studies.
26369027	7	62	from	increase	938:945	arg1	concentration					953:965	Mg concentration	950:965	Mg concentration	950:965	The corrosion rate of the implant decreased with increase in Mg concentration.
26369027	1	63	theme	ultrasonication	132:146	arg1	process					148:154	ultrasonication process	132:154	ultrasonication process	132:154	In this investigation, ultrasonication process was used for the synthesis of magnesium doped nano-hydroxyapatite (MH) (0, 1, 2, and 3 mol% of Mg concentration) particles with controlled size and surface morphology.
26369027	4	64	theme	microporous	615:625	arg1	particles					631:639	microporous HAp particles	615:639	microporous HAp particles	615:639	On incorporation of Mg in HAp lattice, an increase of 20-66 nm in specific surface area was observed in microporous HAp particles.
26369027	12	65	theme	coated	1670:1675	arg1	implant					1677:1683	the MH/CTS nanocomposites coated implant	1644:1683	the MH/CTS nanocomposites coated implant	1644:1683	From this result, it was concluded that the MH/CTS nanocomposites coated implant is the excellent material for implants.
26369027	12	65	theme	coated	1670:1675	arg1	material					1702:1709	the excellent material	1688:1709	the excellent material for implants	1688:1722	From this result, it was concluded that the MH/CTS nanocomposites coated implant is the excellent material for implants.
26369027	3	66	from	nm	507:508	arg1	distribution					480:491	the particle size distribution	462:491	the particle size distribution from 60 to 40 nm	462:508	Increase in the concentration of Mg reduced the particle size distribution from 60 to 40 nm.
26369027	0	67	theme	Biomedical	84:93	arg1	Applications					95:106	Biomedical Applications	84:106	Biomedical Applications	84:106	Mg-Doped Hydroxyapatite/Chitosan Composite Coated 316L Stainless Steel Implants for Biomedical Applications.
26369027	9	68	theme	bacterial	1201:1209	arg1	strains					1211:1217	gram-positive and gram-negative bacterial strains	1169:1217	gram-positive and gram-negative bacterial strains	1169:1217	The antimicrobial activity of the MH/CTS composites against gram-positive and gram-negative bacterial strains indicated that increasing Mg concentration enhanced antimicrobial properties.
26369027	11	69	theme	NIH	1482:1484	arg1	3T3					1486:1488	NIH 3T3	1482:1488	NIH 3T3 fibroblast	1482:1499	From the cytotoxicity analysis against NIH 3T3 fibroblast, it was observed that the Mg incorporated HAp/CTS composite was less toxic than the MHO/CTS composite.
26369027	5	70	theme	XRF	642:644	arg1	patterns					654:661	XRF and XRD patterns	642:661	XRF and XRD patterns	642:661	XRF and XRD patterns reveal that the particles possess stoichiometric composition with reduced crystallinity with respect to the Mg concentration.
26369027	9	71	theme	increasing	1234:1243	arg1	concentration					1248:1260	increasing Mg concentration	1234:1260	increasing Mg concentration	1234:1260	The antimicrobial activity of the MH/CTS composites against gram-positive and gram-negative bacterial strains indicated that increasing Mg concentration enhanced antimicrobial properties.
26369027	12	72	theme	MH/CTS	1648:1653	arg1	implant					1677:1683	the MH/CTS nanocomposites coated implant	1644:1683	the MH/CTS nanocomposites coated implant	1644:1683	From this result, it was concluded that the MH/CTS nanocomposites coated implant is the excellent material for implants.
26369027	12	72	theme	MH/CTS	1648:1653	arg1	material					1702:1709	the excellent material	1688:1709	the excellent material for implants	1688:1722	From this result, it was concluded that the MH/CTS nanocomposites coated implant is the excellent material for implants.
26369027	10	73	theme	implant	1340:1346	arg1	surface					1348:1354	apatite coated implant surface	1325:1354	apatite coated implant surface	1325:1354	Nanoindentation analysis of apatite coated implant surface reveals that the mechanical property depends on the concentration of magnesium in HAp.
26369027	4	74	from	incorporation	514:526	arg1	lattice					541:547	HAp lattice	537:547	HAp lattice	537:547	On incorporation of Mg in HAp lattice, an increase of 20-66 nm in specific surface area was observed in microporous HAp particles.
26369027	8	75	theme	in	972:973	arg1	formation					981:989	The in vitro formation	968:989	The in vitro formation of bonelike apatite layer on the surface of the MH/CTS coated implant	968:1059	The in vitro formation of bonelike apatite layer on the surface of the MH/CTS coated implant was observed from simulated body fluid studies.
26369027	4	76	located	observed	603:610	arg1	particles					631:639	microporous HAp particles	615:639	microporous HAp particles	615:639	On incorporation of Mg in HAp lattice, an increase of 20-66 nm in specific surface area was observed in microporous HAp particles.
26369027	4	76	located	observed	603:610	arg1	incorporation					514:526	incorporation	514:526	incorporation of Mg in HAp lattice	514:547	On incorporation of Mg in HAp lattice, an increase of 20-66 nm in specific surface area was observed in microporous HAp particles.
26369027	4	76	located	observed	603:610	arg2	increase					553:560	an increase	550:560	an increase of 20-66 nm in specific surface area	550:597	On incorporation of Mg in HAp lattice, an increase of 20-66 nm in specific surface area was observed in microporous HAp particles.
26369027	2	77	theme	MH	349:350	arg1	particles					352:360	the prepared MH particles	336:360	the prepared MH particles	336:360	The size of the prepared MH particles was in the range of 20-100 nm with narrow distribution.
26369027	5	78	theme	reduced	729:735	arg1	crystallinity					737:749	reduced crystallinity	729:749	reduced crystallinity with respect to the Mg concentration	729:786	XRF and XRD patterns reveal that the particles possess stoichiometric composition with reduced crystallinity with respect to the Mg concentration.
26369027	9	79	theme	antimicrobial	1271:1283	arg1	properties					1285:1294	antimicrobial properties	1271:1294	antimicrobial properties	1271:1294	The antimicrobial activity of the MH/CTS composites against gram-positive and gram-negative bacterial strains indicated that increasing Mg concentration enhanced antimicrobial properties.
26369027	2	80	theme	nm	389:390	arg1	range					373:377	the range	369:377	the range of 20-100 nm with narrow distribution	369:415	The size of the prepared MH particles was in the range of 20-100 nm with narrow distribution.
26369027	0	81	theme	Coated	43:48	arg1	Implants					71:78	Coated 316L Stainless Steel Implants	43:78	Coated 316L Stainless Steel Implants	43:78	Mg-Doped Hydroxyapatite/Chitosan Composite Coated 316L Stainless Steel Implants for Biomedical Applications.
26369027	8	82	theme	bonelike	994:1001	arg1	layer					1011:1015	bonelike apatite layer	994:1015	bonelike apatite layer	994:1015	The in vitro formation of bonelike apatite layer on the surface of the MH/CTS coated implant was observed from simulated body fluid studies.
26369027	0	83	theme	Stainless	55:63	arg1	Implants					71:78	Coated 316L Stainless Steel Implants	43:78	Coated 316L Stainless Steel Implants	43:78	Mg-Doped Hydroxyapatite/Chitosan Composite Coated 316L Stainless Steel Implants for Biomedical Applications.
26369027	1	84	theme	concentration	254:266	arg1	%					246:246	0, 1, 2, and 3 mol%	228:246	0, 1, 2, and 3 mol%	228:246	In this investigation, ultrasonication process was used for the synthesis of magnesium doped nano-hydroxyapatite (MH) (0, 1, 2, and 3 mol% of Mg concentration) particles with controlled size and surface morphology.
26369027	1	84	theme	concentration	254:266	arg1	concentration					254:266	Mg concentration	251:266	Mg concentration	251:266	In this investigation, ultrasonication process was used for the synthesis of magnesium doped nano-hydroxyapatite (MH) (0, 1, 2, and 3 mol% of Mg concentration) particles with controlled size and surface morphology.
26369027	8	85	theme	layer	1011:1015	arg1	formation					981:989	The in vitro formation	968:989	The in vitro formation of bonelike apatite layer on the surface of the MH/CTS coated implant	968:1059	The in vitro formation of bonelike apatite layer on the surface of the MH/CTS coated implant was observed from simulated body fluid studies.
26369027	1	86	theme	particles	269:277	arg1	synthesis					173:181	the synthesis	169:181	the synthesis of magnesium doped nano-hydroxyapatite (MH) (0, 1, 2, and 3 mol% of Mg concentration) particles with controlled size and surface morphology	169:321	In this investigation, ultrasonication process was used for the synthesis of magnesium doped nano-hydroxyapatite (MH) (0, 1, 2, and 3 mol% of Mg concentration) particles with controlled size and surface morphology.
26369027	2	87	theme	narrow	397:402	arg1	distribution					404:415	narrow distribution	397:415	narrow distribution	397:415	The size of the prepared MH particles was in the range of 20-100 nm with narrow distribution.
26369027	1	88	theme	controlled	284:293	arg1	size					295:298	controlled size	284:298	controlled size	284:298	In this investigation, ultrasonication process was used for the synthesis of magnesium doped nano-hydroxyapatite (MH) (0, 1, 2, and 3 mol% of Mg concentration) particles with controlled size and surface morphology.
26369027	4	89	theme	HAp	537:539	arg1	lattice					541:547	HAp lattice	537:547	HAp lattice	537:547	On incorporation of Mg in HAp lattice, an increase of 20-66 nm in specific surface area was observed in microporous HAp particles.
26369027	11	90	theme	MHO/CTS	1585:1591	arg1	composite					1593:1601	the MHO/CTS composite	1581:1601	the MHO/CTS composite	1581:1601	From the cytotoxicity analysis against NIH 3T3 fibroblast, it was observed that the Mg incorporated HAp/CTS composite was less toxic than the MHO/CTS composite.
26369027	8	91	theme	MH/CTS	1039:1044	arg1	implant					1053:1059	the MH/CTS coated implant	1035:1059	the MH/CTS coated implant	1035:1059	The in vitro formation of bonelike apatite layer on the surface of the MH/CTS coated implant was observed from simulated body fluid studies.
26369027	10	92	theme	apatite	1325:1331	arg1	surface					1348:1354	apatite coated implant surface	1325:1354	apatite coated implant surface	1325:1354	Nanoindentation analysis of apatite coated implant surface reveals that the mechanical property depends on the concentration of magnesium in HAp.
26369027	3	93	theme	particle	466:473	arg1	distribution					480:491	the particle size distribution	462:491	the particle size distribution from 60 to 40 nm	462:508	Increase in the concentration of Mg reduced the particle size distribution from 60 to 40 nm.
23731085	0	0	theme	dissolution	77:87	arg1	rate					89:92	in vitro dissolution rate	68:92	in vitro dissolution rate of poorly water-soluble model drug aripiprazole	68:140	Comparison of nanomilling and coprecipitation on the enhancement of in vitro dissolution rate of poorly water-soluble model drug aripiprazole.
23731085	6	1	from	PVP	879:881	arg1	comparable					900:909	comparable	900:909	comparable	900:909	The saturation solubility of aripiprazole from nanoparticle (NP) and coprecipitate (CP) from PVP and Pluronic was comparable, however, NP of HPC containing composition showed higher solubility when compared to its CP compositions.
23731085	6	1	from	PVP	879:881	arg1	solubility					801:810	The saturation solubility	786:810	The saturation solubility of aripiprazole from nanoparticle (NP) and coprecipitate (CP) from PVP and Pluronic	786:894	The saturation solubility of aripiprazole from nanoparticle (NP) and coprecipitate (CP) from PVP and Pluronic was comparable, however, NP of HPC containing composition showed higher solubility when compared to its CP compositions.
23731085	2	2	theme	physical	371:378	arg1	mixing					380:385	physical mixing	371:385	physical mixing	371:385	Aripiprazole compositions were prepared by physical mixing, coprecipitation and nanomilling using hydroxypropylcellulose (HPC), polyvinylpyrrolidone (PVP) K17 and pluronic F127.
23731085	0	3	from	Comparison	0:9	arg1	enhancement					53:63	the enhancement	49:63	the enhancement of in vitro dissolution rate of poorly water-soluble model drug aripiprazole	49:140	Comparison of nanomilling and coprecipitation on the enhancement of in vitro dissolution rate of poorly water-soluble model drug aripiprazole.
23731085	6	4	theme	aripiprazole	815:826	arg1	comparable					900:909	comparable	900:909	comparable	900:909	The saturation solubility of aripiprazole from nanoparticle (NP) and coprecipitate (CP) from PVP and Pluronic was comparable, however, NP of HPC containing composition showed higher solubility when compared to its CP compositions.
23731085	6	4	theme	aripiprazole	815:826	arg1	solubility					801:810	The saturation solubility	786:810	The saturation solubility of aripiprazole from nanoparticle (NP) and coprecipitate (CP) from PVP and Pluronic	786:894	The saturation solubility of aripiprazole from nanoparticle (NP) and coprecipitate (CP) from PVP and Pluronic was comparable, however, NP of HPC containing composition showed higher solubility when compared to its CP compositions.
23731085	4	5	theme	dissolution	646:656	arg1	rate					658:661	dissolution rate	646:661	dissolution rate	646:661	Aripiprazole pluronic compositions were compressed into tablets and dissolution rate was evaluated.
23731085	0	6	theme	rate	89:92	arg1	enhancement					53:63	the enhancement	49:63	the enhancement of in vitro dissolution rate of poorly water-soluble model drug aripiprazole	49:140	Comparison of nanomilling and coprecipitation on the enhancement of in vitro dissolution rate of poorly water-soluble model drug aripiprazole.
23731085	1	7	dep	in	295:296	arg1	vitro					298:302	vitro	298:302	vitro	298:302	The aim of this study was to evaluate the effect of coprecipitation and nanomilling on the crystallinity of a model drug, aripiprazole and evaluate the in vitro dissolution rate (IDR).
23731085	1	8	theme	model	253:257	arg1	aripiprazole					265:276	aripiprazole	265:276	aripiprazole	265:276	The aim of this study was to evaluate the effect of coprecipitation and nanomilling on the crystallinity of a model drug, aripiprazole and evaluate the in vitro dissolution rate (IDR).
23731085	1	8	theme	model	253:257	arg1	drug					259:262	a model drug	251:262	a model drug	251:262	The aim of this study was to evaluate the effect of coprecipitation and nanomilling on the crystallinity of a model drug, aripiprazole and evaluate the in vitro dissolution rate (IDR).
23731085	0	9	theme	water-soluble	104:116	arg1	aripiprazole					129:140	poorly water-soluble model drug aripiprazole	97:140	poorly water-soluble model drug aripiprazole	97:140	Comparison of nanomilling and coprecipitation on the enhancement of in vitro dissolution rate of poorly water-soluble model drug aripiprazole.
23731085	9	10	dep	tablets	1394:1400	arg1	coprecipitate					1437:1449	coprecipitate	1437:1449	coprecipitate powders	1437:1457	Aripiprazole tablets prepared from nanomilled powder dissolved >75% within 10 min compared with 17% and 20% for tablets prepared from physical mixture and coprecipitate powders, respectively.
23731085	9	10	dep	tablets	1394:1400	arg1	prepared					1402:1409	prepared	1402:1409	prepared from physical mixture	1402:1431	Aripiprazole tablets prepared from nanomilled powder dissolved >75% within 10 min compared with 17% and 20% for tablets prepared from physical mixture and coprecipitate powders, respectively.
23731085	6	11	from	nanoparticle	833:844	arg1	comparable					900:909	comparable	900:909	comparable	900:909	The saturation solubility of aripiprazole from nanoparticle (NP) and coprecipitate (CP) from PVP and Pluronic was comparable, however, NP of HPC containing composition showed higher solubility when compared to its CP compositions.
23731085	6	11	from	nanoparticle	833:844	arg1	aripiprazole					815:826	aripiprazole	815:826	aripiprazole from nanoparticle (NP) and coprecipitate (CP)	815:872	The saturation solubility of aripiprazole from nanoparticle (NP) and coprecipitate (CP) from PVP and Pluronic was comparable, however, NP of HPC containing composition showed higher solubility when compared to its CP compositions.
23731085	6	11	from	nanoparticle	833:844	arg1	solubility					801:810	The saturation solubility	786:810	The saturation solubility of aripiprazole from nanoparticle (NP) and coprecipitate (CP) from PVP and Pluronic	786:894	The saturation solubility of aripiprazole from nanoparticle (NP) and coprecipitate (CP) from PVP and Pluronic was comparable, however, NP of HPC containing composition showed higher solubility when compared to its CP compositions.
23731085	8	12	theme	coprecipitate	1193:1205	arg1	compositions					1207:1218	coprecipitate compositions	1193:1218	coprecipitate compositions	1193:1218	The increased aripiprazole IDR was due to decreased crystallinity from coprecipitate compositions and disruption of crystallinity from nanomilled compositions.
23731085	1	13	theme	study	159:163	arg1	aim					147:149	The aim	143:149	The aim of this study	143:163	The aim of this study was to evaluate the effect of coprecipitation and nanomilling on the crystallinity of a model drug, aripiprazole and evaluate the in vitro dissolution rate (IDR).
23731085	6	14	theme	higher	961:966	arg1	solubility					968:977	higher solubility	961:977	higher solubility	961:977	The saturation solubility of aripiprazole from nanoparticle (NP) and coprecipitate (CP) from PVP and Pluronic was comparable, however, NP of HPC containing composition showed higher solubility when compared to its CP compositions.
23731085	8	15	from	disruption	1224:1233	arg1	compositions					1268:1279	nanomilled compositions	1257:1279	nanomilled compositions	1257:1279	The increased aripiprazole IDR was due to decreased crystallinity from coprecipitate compositions and disruption of crystallinity from nanomilled compositions.
23731085	6	16	theme	saturation	790:799	arg1	comparable					900:909	comparable	900:909	comparable	900:909	The saturation solubility of aripiprazole from nanoparticle (NP) and coprecipitate (CP) from PVP and Pluronic was comparable, however, NP of HPC containing composition showed higher solubility when compared to its CP compositions.
23731085	6	16	theme	saturation	790:799	arg1	solubility					801:810	The saturation solubility	786:810	The saturation solubility of aripiprazole from nanoparticle (NP) and coprecipitate (CP) from PVP and Pluronic	786:894	The saturation solubility of aripiprazole from nanoparticle (NP) and coprecipitate (CP) from PVP and Pluronic was comparable, however, NP of HPC containing composition showed higher solubility when compared to its CP compositions.
23731085	3	17	theme	particle	510:517	arg1	size					519:522	The particle size	506:522	The particle size	506:522	The particle size, solubility, IDR and drug crystallinity were studied.
23731085	2	18	theme	pluronic	491:498	arg1	hydroxypropylcellulose					426:447	hydroxypropylcellulose	426:447	hydroxypropylcellulose	426:447	Aripiprazole compositions were prepared by physical mixing, coprecipitation and nanomilling using hydroxypropylcellulose (HPC), polyvinylpyrrolidone (PVP) K17 and pluronic F127.
23731085	2	18	theme	pluronic	491:498	arg1	F127					500:503	pluronic F127	491:503	pluronic F127	491:503	Aripiprazole compositions were prepared by physical mixing, coprecipitation and nanomilling using hydroxypropylcellulose (HPC), polyvinylpyrrolidone (PVP) K17 and pluronic F127.
23731085	6	19	from	coprecipitate	855:867	arg1	comparable					900:909	comparable	900:909	comparable	900:909	The saturation solubility of aripiprazole from nanoparticle (NP) and coprecipitate (CP) from PVP and Pluronic was comparable, however, NP of HPC containing composition showed higher solubility when compared to its CP compositions.
23731085	6	19	from	coprecipitate	855:867	arg1	aripiprazole					815:826	aripiprazole	815:826	aripiprazole from nanoparticle (NP) and coprecipitate (CP)	815:872	The saturation solubility of aripiprazole from nanoparticle (NP) and coprecipitate (CP) from PVP and Pluronic was comparable, however, NP of HPC containing composition showed higher solubility when compared to its CP compositions.
23731085	6	19	from	coprecipitate	855:867	arg1	solubility					801:810	The saturation solubility	786:810	The saturation solubility of aripiprazole from nanoparticle (NP) and coprecipitate (CP) from PVP and Pluronic	786:894	The saturation solubility of aripiprazole from nanoparticle (NP) and coprecipitate (CP) from PVP and Pluronic was comparable, however, NP of HPC containing composition showed higher solubility when compared to its CP compositions.
23731085	5	20	theme	other	766:770	arg1	compositions					772:783	the other compositions	762:783	the other compositions	762:783	The particle size of nanomilled compositions was significantly smaller than that of the other compositions.
23731085	8	21	theme	increased	1126:1134	arg1	due					1157:1159	due	1157:1159	due	1157:1159	The increased aripiprazole IDR was due to decreased crystallinity from coprecipitate compositions and disruption of crystallinity from nanomilled compositions.
23731085	8	21	theme	increased	1126:1134	arg1	IDR					1149:1151	The increased aripiprazole IDR	1122:1151	The increased aripiprazole IDR	1122:1151	The increased aripiprazole IDR was due to decreased crystallinity from coprecipitate compositions and disruption of crystallinity from nanomilled compositions.
23731085	6	22	theme	CP	1000:1001	arg1	compositions					1003:1014	its CP compositions	996:1014	its CP compositions	996:1014	The saturation solubility of aripiprazole from nanoparticle (NP) and coprecipitate (CP) from PVP and Pluronic was comparable, however, NP of HPC containing composition showed higher solubility when compared to its CP compositions.
23731085	1	23	theme	drug	259:262	arg1	crystallinity					234:246	the crystallinity	230:246	the crystallinity of a model drug, aripiprazole	230:276	The aim of this study was to evaluate the effect of coprecipitation and nanomilling on the crystallinity of a model drug, aripiprazole and evaluate the in vitro dissolution rate (IDR).
23731085	8	24	theme	nanomilled	1257:1266	arg1	compositions					1268:1279	nanomilled compositions	1257:1279	nanomilled compositions	1257:1279	The increased aripiprazole IDR was due to decreased crystallinity from coprecipitate compositions and disruption of crystallinity from nanomilled compositions.
23731085	6	25	theme	composition	942:952	arg1	NP					921:922	NP	921:922	NP of HPC containing composition	921:952	The saturation solubility of aripiprazole from nanoparticle (NP) and coprecipitate (CP) from PVP and Pluronic was comparable, however, NP of HPC containing composition showed higher solubility when compared to its CP compositions.
23731085	0	26	theme	drug	124:127	arg1	aripiprazole					129:140	poorly water-soluble model drug aripiprazole	97:140	poorly water-soluble model drug aripiprazole	97:140	Comparison of nanomilling and coprecipitation on the enhancement of in vitro dissolution rate of poorly water-soluble model drug aripiprazole.
23731085	0	27	dep	in	68:69	arg1	vitro					71:75	vitro	71:75	vitro	71:75	Comparison of nanomilling and coprecipitation on the enhancement of in vitro dissolution rate of poorly water-soluble model drug aripiprazole.
23731085	0	28	theme	nanomilling	14:24	arg1	Comparison					0:9	Comparison	0:9	Comparison of nanomilling and coprecipitation on the enhancement of in vitro dissolution rate of poorly water-soluble model drug aripiprazole.	0:141	Comparison of nanomilling and coprecipitation on the enhancement of in vitro dissolution rate of poorly water-soluble model drug aripiprazole.
23731085	6	29	theme	containing	931:940	arg1	composition					942:952	HPC containing composition	927:952	HPC containing composition	927:952	The saturation solubility of aripiprazole from nanoparticle (NP) and coprecipitate (CP) from PVP and Pluronic was comparable, however, NP of HPC containing composition showed higher solubility when compared to its CP compositions.
23731085	0	30	theme	model	118:122	arg1	aripiprazole					129:140	poorly water-soluble model drug aripiprazole	97:140	poorly water-soluble model drug aripiprazole	97:140	Comparison of nanomilling and coprecipitation on the enhancement of in vitro dissolution rate of poorly water-soluble model drug aripiprazole.
23731085	9	31	theme	nanomilled	1317:1326	arg1	powder					1328:1333	nanomilled powder	1317:1333	nanomilled powder	1317:1333	Aripiprazole tablets prepared from nanomilled powder dissolved >75% within 10 min compared with 17% and 20% for tablets prepared from physical mixture and coprecipitate powders, respectively.
23731085	2	32	theme	polyvinylpyrrolidone	456:475	arg1	hydroxypropylcellulose					426:447	hydroxypropylcellulose	426:447	hydroxypropylcellulose	426:447	Aripiprazole compositions were prepared by physical mixing, coprecipitation and nanomilling using hydroxypropylcellulose (HPC), polyvinylpyrrolidone (PVP) K17 and pluronic F127.
23731085	2	32	theme	polyvinylpyrrolidone	456:475	arg1	K17					483:485	polyvinylpyrrolidone (PVP) K17	456:485	polyvinylpyrrolidone (PVP) K17	456:485	Aripiprazole compositions were prepared by physical mixing, coprecipitation and nanomilling using hydroxypropylcellulose (HPC), polyvinylpyrrolidone (PVP) K17 and pluronic F127.
23731085	6	33	theme	HPC	927:929	arg1	composition					942:952	HPC containing composition	927:952	HPC containing composition	927:952	The saturation solubility of aripiprazole from nanoparticle (NP) and coprecipitate (CP) from PVP and Pluronic was comparable, however, NP of HPC containing composition showed higher solubility when compared to its CP compositions.
23731085	5	34	theme	nanomilled	699:708	arg1	compositions					710:721	nanomilled compositions	699:721	nanomilled compositions	699:721	The particle size of nanomilled compositions was significantly smaller than that of the other compositions.
23731085	7	35	theme	physical	1066:1073	arg1	mixtures					1075:1082	physical mixtures	1066:1082	physical mixtures	1066:1082	The crystallinity of aripiprazole decreased from physical mixtures to coprecipitates and further in NPs.
23731085	0	36	theme	coprecipitation	30:44	arg1	Comparison					0:9	Comparison	0:9	Comparison of nanomilling and coprecipitation on the enhancement of in vitro dissolution rate of poorly water-soluble model drug aripiprazole.	0:141	Comparison of nanomilling and coprecipitation on the enhancement of in vitro dissolution rate of poorly water-soluble model drug aripiprazole.
23731085	0	37	theme	aripiprazole	129:140	arg1	rate					89:92	in vitro dissolution rate	68:92	in vitro dissolution rate of poorly water-soluble model drug aripiprazole	68:140	Comparison of nanomilling and coprecipitation on the enhancement of in vitro dissolution rate of poorly water-soluble model drug aripiprazole.
23731085	8	38	theme	aripiprazole	1136:1147	arg1	due					1157:1159	due	1157:1159	due	1157:1159	The increased aripiprazole IDR was due to decreased crystallinity from coprecipitate compositions and disruption of crystallinity from nanomilled compositions.
23731085	8	38	theme	aripiprazole	1136:1147	arg1	IDR					1149:1151	The increased aripiprazole IDR	1122:1151	The increased aripiprazole IDR	1122:1151	The increased aripiprazole IDR was due to decreased crystallinity from coprecipitate compositions and disruption of crystallinity from nanomilled compositions.
23731085	9	39	theme	physical	1416:1423	arg1	mixture					1425:1431	physical mixture	1416:1431	physical mixture	1416:1431	Aripiprazole tablets prepared from nanomilled powder dissolved >75% within 10 min compared with 17% and 20% for tablets prepared from physical mixture and coprecipitate powders, respectively.
23731085	1	40	theme	coprecipitation	195:209	arg1	effect					185:190	the effect	181:190	the effect of coprecipitation and nanomilling on the crystallinity of a model drug, aripiprazole	181:276	The aim of this study was to evaluate the effect of coprecipitation and nanomilling on the crystallinity of a model drug, aripiprazole and evaluate the in vitro dissolution rate (IDR).
23731085	8	41	theme	crystallinity	1238:1250	arg1	disruption					1224:1233	disruption	1224:1233	disruption of crystallinity from nanomilled compositions	1224:1279	The increased aripiprazole IDR was due to decreased crystallinity from coprecipitate compositions and disruption of crystallinity from nanomilled compositions.
23731085	8	41	theme	crystallinity	1238:1250	arg1	crystallinity					1174:1186	decreased crystallinity	1164:1186	decreased crystallinity from coprecipitate compositions	1164:1218	The increased aripiprazole IDR was due to decreased crystallinity from coprecipitate compositions and disruption of crystallinity from nanomilled compositions.
23731085	3	42	theme	drug	545:548	arg1	crystallinity					550:562	drug crystallinity	545:562	drug crystallinity	545:562	The particle size, solubility, IDR and drug crystallinity were studied.
23731085	8	43	from	compositions	1268:1279	arg1	disruption					1224:1233	disruption	1224:1233	disruption of crystallinity from nanomilled compositions	1224:1279	The increased aripiprazole IDR was due to decreased crystallinity from coprecipitate compositions and disruption of crystallinity from nanomilled compositions.
23731085	8	43	from	compositions	1268:1279	arg1	crystallinity					1174:1186	decreased crystallinity	1164:1186	decreased crystallinity from coprecipitate compositions	1164:1218	The increased aripiprazole IDR was due to decreased crystallinity from coprecipitate compositions and disruption of crystallinity from nanomilled compositions.
23731085	8	43	from	compositions	1268:1279	arg1	crystallinity					1238:1250	crystallinity	1238:1250	crystallinity from nanomilled compositions	1238:1279	The increased aripiprazole IDR was due to decreased crystallinity from coprecipitate compositions and disruption of crystallinity from nanomilled compositions.
23731085	5	44	theme	compositions	710:721	arg1	smaller					741:747	smaller	741:747	smaller	741:747	The particle size of nanomilled compositions was significantly smaller than that of the other compositions.
23731085	5	44	theme	compositions	710:721	arg1	size					691:694	The particle size	678:694	The particle size of nanomilled compositions	678:721	The particle size of nanomilled compositions was significantly smaller than that of the other compositions.
23731085	1	45	theme	in	295:296	arg1	IDR					322:324	IDR	322:324	IDR	322:324	The aim of this study was to evaluate the effect of coprecipitation and nanomilling on the crystallinity of a model drug, aripiprazole and evaluate the in vitro dissolution rate (IDR).
23731085	1	45	theme	in	295:296	arg1	rate					316:319	the in vitro dissolution rate	291:319	the in vitro dissolution rate (IDR)	291:325	The aim of this study was to evaluate the effect of coprecipitation and nanomilling on the crystallinity of a model drug, aripiprazole and evaluate the in vitro dissolution rate (IDR).
23731085	8	46	from	compositions	1207:1218	arg1	disruption					1224:1233	disruption	1224:1233	disruption of crystallinity from nanomilled compositions	1224:1279	The increased aripiprazole IDR was due to decreased crystallinity from coprecipitate compositions and disruption of crystallinity from nanomilled compositions.
23731085	8	46	from	compositions	1207:1218	arg1	crystallinity					1174:1186	decreased crystallinity	1164:1186	decreased crystallinity from coprecipitate compositions	1164:1218	The increased aripiprazole IDR was due to decreased crystallinity from coprecipitate compositions and disruption of crystallinity from nanomilled compositions.
23731085	9	47	theme	Aripiprazole	1282:1293	arg1	tablets					1295:1301	Aripiprazole tablets	1282:1301	Aripiprazole tablets prepared from nanomilled powder	1282:1333	Aripiprazole tablets prepared from nanomilled powder dissolved >75% within 10 min compared with 17% and 20% for tablets prepared from physical mixture and coprecipitate powders, respectively.
23731085	1	48	from	effect	185:190	arg1	crystallinity					234:246	the crystallinity	230:246	the crystallinity of a model drug, aripiprazole	230:276	The aim of this study was to evaluate the effect of coprecipitation and nanomilling on the crystallinity of a model drug, aripiprazole and evaluate the in vitro dissolution rate (IDR).
23731085	4	49	theme	Aripiprazole	578:589	arg1	compositions					600:611	Aripiprazole pluronic compositions	578:611	Aripiprazole pluronic compositions	578:611	Aripiprazole pluronic compositions were compressed into tablets and dissolution rate was evaluated.
23731085	5	50	theme	particle	682:689	arg1	smaller					741:747	smaller	741:747	smaller	741:747	The particle size of nanomilled compositions was significantly smaller than that of the other compositions.
23731085	5	50	theme	particle	682:689	arg1	size					691:694	The particle size	678:694	The particle size of nanomilled compositions	678:721	The particle size of nanomilled compositions was significantly smaller than that of the other compositions.
23731085	1	51	theme	nanomilling	215:225	arg1	effect					185:190	the effect	181:190	the effect of coprecipitation and nanomilling on the crystallinity of a model drug, aripiprazole	181:276	The aim of this study was to evaluate the effect of coprecipitation and nanomilling on the crystallinity of a model drug, aripiprazole and evaluate the in vitro dissolution rate (IDR).
23731085	0	52	theme	in	68:69	arg1	rate					89:92	in vitro dissolution rate	68:92	in vitro dissolution rate of poorly water-soluble model drug aripiprazole	68:140	Comparison of nanomilling and coprecipitation on the enhancement of in vitro dissolution rate of poorly water-soluble model drug aripiprazole.
23731085	8	53	theme	decreased	1164:1172	arg1	crystallinity					1174:1186	decreased crystallinity	1164:1186	decreased crystallinity from coprecipitate compositions	1164:1218	The increased aripiprazole IDR was due to decreased crystallinity from coprecipitate compositions and disruption of crystallinity from nanomilled compositions.
23731085	6	54	from	solubility	801:810	arg1	NP					847:848	NP	847:848	NP	847:848	The saturation solubility of aripiprazole from nanoparticle (NP) and coprecipitate (CP) from PVP and Pluronic was comparable, however, NP of HPC containing composition showed higher solubility when compared to its CP compositions.
23731085	6	54	from	solubility	801:810	arg1	coprecipitate					855:867	coprecipitate	855:867	coprecipitate	855:867	The saturation solubility of aripiprazole from nanoparticle (NP) and coprecipitate (CP) from PVP and Pluronic was comparable, however, NP of HPC containing composition showed higher solubility when compared to its CP compositions.
23731085	6	54	from	solubility	801:810	arg1	nanoparticle					833:844	nanoparticle	833:844	nanoparticle (NP)	833:849	The saturation solubility of aripiprazole from nanoparticle (NP) and coprecipitate (CP) from PVP and Pluronic was comparable, however, NP of HPC containing composition showed higher solubility when compared to its CP compositions.
23731085	6	54	from	solubility	801:810	arg1	CP					870:871	CP	870:871	CP	870:871	The saturation solubility of aripiprazole from nanoparticle (NP) and coprecipitate (CP) from PVP and Pluronic was comparable, however, NP of HPC containing composition showed higher solubility when compared to its CP compositions.
23731085	8	55	from	crystallinity	1174:1186	arg1	compositions					1268:1279	nanomilled compositions	1257:1279	nanomilled compositions	1257:1279	The increased aripiprazole IDR was due to decreased crystallinity from coprecipitate compositions and disruption of crystallinity from nanomilled compositions.
23731085	6	56	from	Pluronic	887:894	arg1	comparable					900:909	comparable	900:909	comparable	900:909	The saturation solubility of aripiprazole from nanoparticle (NP) and coprecipitate (CP) from PVP and Pluronic was comparable, however, NP of HPC containing composition showed higher solubility when compared to its CP compositions.
23731085	6	56	from	Pluronic	887:894	arg1	solubility					801:810	The saturation solubility	786:810	The saturation solubility of aripiprazole from nanoparticle (NP) and coprecipitate (CP) from PVP and Pluronic	786:894	The saturation solubility of aripiprazole from nanoparticle (NP) and coprecipitate (CP) from PVP and Pluronic was comparable, however, NP of HPC containing composition showed higher solubility when compared to its CP compositions.
23731085	1	57	theme	dissolution	304:314	arg1	IDR					322:324	IDR	322:324	IDR	322:324	The aim of this study was to evaluate the effect of coprecipitation and nanomilling on the crystallinity of a model drug, aripiprazole and evaluate the in vitro dissolution rate (IDR).
23731085	1	57	theme	dissolution	304:314	arg1	rate					316:319	the in vitro dissolution rate	291:319	the in vitro dissolution rate (IDR)	291:325	The aim of this study was to evaluate the effect of coprecipitation and nanomilling on the crystallinity of a model drug, aripiprazole and evaluate the in vitro dissolution rate (IDR).
23731085	7	58	theme	aripiprazole	1038:1049	arg1	crystallinity					1021:1033	The crystallinity	1017:1033	The crystallinity of aripiprazole	1017:1049	The crystallinity of aripiprazole decreased from physical mixtures to coprecipitates and further in NPs.
23731085	2	59	theme	Aripiprazole	328:339	arg1	compositions					341:352	Aripiprazole compositions	328:352	Aripiprazole compositions	328:352	Aripiprazole compositions were prepared by physical mixing, coprecipitation and nanomilling using hydroxypropylcellulose (HPC), polyvinylpyrrolidone (PVP) K17 and pluronic F127.
23731085	10	60	from	increase	1478:1485	arg1	IDR					1490:1492	IDR	1490:1492	IDR	1490:1492	The increase in IDR due to nanomilling was more significant than coprecipitation and NPs retained the IDR after compression into tablets.
23731085	4	61	theme	pluronic	591:598	arg1	compositions					600:611	Aripiprazole pluronic compositions	578:611	Aripiprazole pluronic compositions	578:611	Aripiprazole pluronic compositions were compressed into tablets and dissolution rate was evaluated.
25237774	9	0	theme	fermentation	1088:1099	arg1	residue					1101:1107	the fermentation residue	1084:1107	the fermentation residue prior to anaerobic digestion	1084:1136	Co-production of bioethanol and biogas in which the peels were added to the fermentation residue prior to anaerobic digestion produced maximum fuel energy yield of (15-23MJ/kgVS).
25237774	8	1	from	mix	953:955	arg1	methane					928:934	methane	928:934	methane	928:934	The fuel energy of the bioethanol and methane from flour-peels mix ranged from 5 to 13 and 11 to 14MJ/kgVS, respectively.
25237774	8	1	from	mix	953:955	arg1	energy					899:904	The fuel energy	890:904	The fuel energy of the bioethanol and methane from flour-peels mix	890:955	The fuel energy of the bioethanol and methane from flour-peels mix ranged from 5 to 13 and 11 to 14MJ/kgVS, respectively.
25237774	8	1	from	mix	953:955	arg1	bioethanol					913:922	bioethanol	913:922	bioethanol	913:922	The fuel energy of the bioethanol and methane from flour-peels mix ranged from 5 to 13 and 11 to 14MJ/kgVS, respectively.
25237774	5	2	theme	biogas	656:661	arg1	production					663:672	biogas production	656:672	biogas production	656:672	Composition analysis demonstrated that M. glaziovii is a suitable feedstock for both bioethanol and biogas production.
25237774	6	3	theme	8	784:784	arg1	%					779:779	%	779:779	%	779:779	Starch content ranged from 77% to 81%, structural carbohydrates 3-16%, total crude protein ranged from 2% to 8%.
25237774	1	4	dep	Arg	80:82	arg1	Bioethanol					123:132	Bioethanol	123:132	Bioethanol	123:132	Arg, for production of bioenergy carriers: Bioethanol and biogas.
25237774	1	4	dep	Arg	80:82	arg1	biogas					138:143	biogas	138:143	biogas	138:143	Arg, for production of bioenergy carriers: Bioethanol and biogas.
25237774	7	5	theme	up	841:842	arg1	concentration					824:836	ethanol concentration	816:836	ethanol concentration of up to 85g/L	816:851	Yeast fermentation achieved ethanol concentration of up to 85g/L at a fermentation efficiency of 89%.
25237774	4	6	theme	energy	501:506	arg1	values					508:513	their energy values	495:513	their energy values	495:513	These bioenergy carriers were produced both separately and sequentially and their energy values evaluated based on these two approaches.
25237774	8	7	theme	bioethanol	913:922	arg1	energy					899:904	The fuel energy	890:904	The fuel energy of the bioethanol and methane from flour-peels mix	890:955	The fuel energy of the bioethanol and methane from flour-peels mix ranged from 5 to 13 and 11 to 14MJ/kgVS, respectively.
25237774	8	8	dep	13	974:975	arg1	to					971:972	to	971:972	to	971:972	The fuel energy of the bioethanol and methane from flour-peels mix ranged from 5 to 13 and 11 to 14MJ/kgVS, respectively.
25237774	6	9	theme	81	709:710	arg1	%					704:704	%	704:704	%	704:704	Starch content ranged from 77% to 81%, structural carbohydrates 3-16%, total crude protein ranged from 2% to 8%.
25237774	7	10	theme	ethanol	816:822	arg1	concentration					824:836	ethanol concentration	816:836	ethanol concentration of up to 85g/L	816:851	Yeast fermentation achieved ethanol concentration of up to 85g/L at a fermentation efficiency of 89%.
25237774	8	11	theme	flour-peels	941:951	arg1	mix					953:955	flour-peels mix	941:955	flour-peels mix	941:955	The fuel energy of the bioethanol and methane from flour-peels mix ranged from 5 to 13 and 11 to 14MJ/kgVS, respectively.
25237774	5	12	theme	suitable	613:620	arg1	feedstock					622:630	a suitable feedstock	611:630	a suitable feedstock for both bioethanol and biogas production	611:672	Composition analysis demonstrated that M. glaziovii is a suitable feedstock for both bioethanol and biogas production.
25237774	5	12	theme	suitable	613:620	arg1	glaziovii					598:606	M. glaziovii	595:606	M. glaziovii	595:606	Composition analysis demonstrated that M. glaziovii is a suitable feedstock for both bioethanol and biogas production.
25237774	3	13	theme	different	320:328	arg1	areas					330:334	3 different areas	318:334	3 different areas in Tanzania	318:346	Tubers obtained from 3 different areas in Tanzania were characterised and evaluated for bioethanol and biogas production.
25237774	6	14	dep	%	704:704	arg1	to					706:707	to	706:707	to	706:707	Starch content ranged from 77% to 81%, structural carbohydrates 3-16%, total crude protein ranged from 2% to 8%.
25237774	3	15	dep	bioethanol	385:394	arg1	production					407:416	production	407:416	production	407:416	Tubers obtained from 3 different areas in Tanzania were characterised and evaluated for bioethanol and biogas production.
25237774	7	16	theme	%	887:887	arg1	efficiency					871:880	a fermentation efficiency	856:880	a fermentation efficiency of 89%	856:887	Yeast fermentation achieved ethanol concentration of up to 85g/L at a fermentation efficiency of 89%.
25237774	6	17	theme	77	702:703	arg1	%					704:704	%	704:704	%	704:704	Starch content ranged from 77% to 81%, structural carbohydrates 3-16%, total crude protein ranged from 2% to 8%.
25237774	9	18	theme	bioethanol	1029:1038	arg1	Co-production					1012:1024	Co-production	1012:1024	Co-production of bioethanol and biogas in which the peels were added to the fermentation residue prior to anaerobic digestion	1012:1136	Co-production of bioethanol and biogas in which the peels were added to the fermentation residue prior to anaerobic digestion produced maximum fuel energy yield of (15-23MJ/kgVS).
25237774	9	19	theme	maximum	1147:1153	arg1	yield					1167:1171	maximum fuel energy yield	1147:1171	maximum fuel energy yield of (15-23MJ/kgVS)	1147:1189	Co-production of bioethanol and biogas in which the peels were added to the fermentation residue prior to anaerobic digestion produced maximum fuel energy yield of (15-23MJ/kgVS).
25237774	2	20	theme	bioenergy	275:283	arg1	production					285:294	bioenergy production	275:294	bioenergy production	275:294	The objective of this study was to characterise and evaluate a wild inedible cassava species, Manihot glaziovii as feedstock for bioenergy production.
25237774	0	21	theme	feedstock	43:51	arg1	evaluation					21:30	evaluation	21:30	evaluation	21:30	Characterisation and evaluation of a novel feedstock, Manihot glaziovii, Muell.
25237774	0	21	theme	feedstock	43:51	arg1	Characterisation					0:15	Characterisation	0:15	Characterisation	0:15	Characterisation and evaluation of a novel feedstock, Manihot glaziovii, Muell.
25237774	5	22	theme	M.	595:596	arg1	feedstock					622:630	a suitable feedstock	611:630	a suitable feedstock for both bioethanol and biogas production	611:672	Composition analysis demonstrated that M. glaziovii is a suitable feedstock for both bioethanol and biogas production.
25237774	5	22	theme	M.	595:596	arg1	glaziovii					598:606	M. glaziovii	595:606	M. glaziovii	595:606	Composition analysis demonstrated that M. glaziovii is a suitable feedstock for both bioethanol and biogas production.
25237774	9	23	theme	prior	1109:1113	arg1	residue					1101:1107	the fermentation residue	1084:1107	the fermentation residue prior to anaerobic digestion	1084:1136	Co-production of bioethanol and biogas in which the peels were added to the fermentation residue prior to anaerobic digestion produced maximum fuel energy yield of (15-23MJ/kgVS).
25237774	8	24	theme	fuel	894:897	arg1	energy					899:904	The fuel energy	890:904	The fuel energy of the bioethanol and methane from flour-peels mix	890:955	The fuel energy of the bioethanol and methane from flour-peels mix ranged from 5 to 13 and 11 to 14MJ/kgVS, respectively.
25237774	9	25	theme	energy	1160:1165	arg1	yield					1167:1171	maximum fuel energy yield	1147:1171	maximum fuel energy yield of (15-23MJ/kgVS)	1147:1189	Co-production of bioethanol and biogas in which the peels were added to the fermentation residue prior to anaerobic digestion produced maximum fuel energy yield of (15-23MJ/kgVS).
25237774	8	26	dep	bioethanol	913:922	arg1	the					909:911	the	909:911	the	909:911	The fuel energy of the bioethanol and methane from flour-peels mix ranged from 5 to 13 and 11 to 14MJ/kgVS, respectively.
25237774	9	27	theme	biogas	1044:1049	arg1	Co-production					1012:1024	Co-production	1012:1024	Co-production of bioethanol and biogas in which the peels were added to the fermentation residue prior to anaerobic digestion	1012:1136	Co-production of bioethanol and biogas in which the peels were added to the fermentation residue prior to anaerobic digestion produced maximum fuel energy yield of (15-23MJ/kgVS).
25237774	6	28	dep	%	779:779	arg1	to					781:782	to	781:782	to	781:782	Starch content ranged from 77% to 81%, structural carbohydrates 3-16%, total crude protein ranged from 2% to 8%.
25237774	0	29	theme	novel	37:41	arg1	Muell					73:77	Muell	73:77	Muell	73:77	Characterisation and evaluation of a novel feedstock, Manihot glaziovii, Muell.
25237774	0	29	theme	novel	37:41	arg1	feedstock					43:51	a novel feedstock	35:51	a novel feedstock	35:51	Characterisation and evaluation of a novel feedstock, Manihot glaziovii, Muell.
25237774	0	29	theme	novel	37:41	arg1	glaziovii					62:70	Manihot glaziovii	54:70	Manihot glaziovii	54:70	Characterisation and evaluation of a novel feedstock, Manihot glaziovii, Muell.
25237774	7	30	theme	Yeast	788:792	arg1	fermentation					794:805	Yeast fermentation	788:805	Yeast fermentation	788:805	Yeast fermentation achieved ethanol concentration of up to 85g/L at a fermentation efficiency of 89%.
25237774	4	31	theme	bioenergy	425:433	arg1	carriers					435:442	These bioenergy carriers	419:442	These bioenergy carriers	419:442	These bioenergy carriers were produced both separately and sequentially and their energy values evaluated based on these two approaches.
25237774	6	32	theme	Starch	675:680	arg1	content					682:688	Starch content	675:688	Starch content	675:688	Starch content ranged from 77% to 81%, structural carbohydrates 3-16%, total crude protein ranged from 2% to 8%.
25237774	8	33	from	energy	899:904	arg1	mix					953:955	flour-peels mix	941:955	flour-peels mix	941:955	The fuel energy of the bioethanol and methane from flour-peels mix ranged from 5 to 13 and 11 to 14MJ/kgVS, respectively.
25237774	3	34	from	areas	330:334	arg1	Tanzania					339:346	Tanzania	339:346	Tanzania	339:346	Tubers obtained from 3 different areas in Tanzania were characterised and evaluated for bioethanol and biogas production.
25237774	6	35	theme	total	746:750	arg1	carbohydrates					725:737	structural carbohydrates	714:737	structural carbohydrates 3-16%	714:743	Starch content ranged from 77% to 81%, structural carbohydrates 3-16%, total crude protein ranged from 2% to 8%.
25237774	6	35	theme	total	746:750	arg1	protein					758:764	total crude protein	746:764	total crude protein ranged from 2% to 8%	746:785	Starch content ranged from 77% to 81%, structural carbohydrates 3-16%, total crude protein ranged from 2% to 8%.
25237774	8	36	theme	methane	928:934	arg1	energy					899:904	The fuel energy	890:904	The fuel energy of the bioethanol and methane from flour-peels mix	890:955	The fuel energy of the bioethanol and methane from flour-peels mix ranged from 5 to 13 and 11 to 14MJ/kgVS, respectively.
25237774	8	37	dep	14MJ/kgVS	987:995	arg1	to					984:985	to	984:985	to	984:985	The fuel energy of the bioethanol and methane from flour-peels mix ranged from 5 to 13 and 11 to 14MJ/kgVS, respectively.
25237774	9	38	theme	fuel	1155:1158	arg1	yield					1167:1171	maximum fuel energy yield	1147:1171	maximum fuel energy yield of (15-23MJ/kgVS)	1147:1189	Co-production of bioethanol and biogas in which the peels were added to the fermentation residue prior to anaerobic digestion produced maximum fuel energy yield of (15-23MJ/kgVS).
25237774	4	39	dep	evaluated	515:523	arg1	based					525:529	based	525:529	evaluated based on these two approaches	515:553	These bioenergy carriers were produced both separately and sequentially and their energy values evaluated based on these two approaches.
25237774	9	40	theme	15-23MJ/kgVS	1177:1188	arg1	yield					1167:1171	maximum fuel energy yield	1147:1171	maximum fuel energy yield of (15-23MJ/kgVS)	1147:1189	Co-production of bioethanol and biogas in which the peels were added to the fermentation residue prior to anaerobic digestion produced maximum fuel energy yield of (15-23MJ/kgVS).
25237774	6	41	theme	%	743:743	arg1	carbohydrates					725:737	structural carbohydrates	714:737	structural carbohydrates 3-16%	714:743	Starch content ranged from 77% to 81%, structural carbohydrates 3-16%, total crude protein ranged from 2% to 8%.
25237774	6	41	theme	%	743:743	arg1	protein					758:764	total crude protein	746:764	total crude protein ranged from 2% to 8%	746:785	Starch content ranged from 77% to 81%, structural carbohydrates 3-16%, total crude protein ranged from 2% to 8%.
25237774	6	42	theme	crude	752:756	arg1	carbohydrates					725:737	structural carbohydrates	714:737	structural carbohydrates 3-16%	714:743	Starch content ranged from 77% to 81%, structural carbohydrates 3-16%, total crude protein ranged from 2% to 8%.
25237774	6	42	theme	crude	752:756	arg1	protein					758:764	total crude protein	746:764	total crude protein ranged from 2% to 8%	746:785	Starch content ranged from 77% to 81%, structural carbohydrates 3-16%, total crude protein ranged from 2% to 8%.
25237774	9	43	theme	anaerobic	1118:1126	arg1	digestion					1128:1136	anaerobic digestion	1118:1136	anaerobic digestion	1118:1136	Co-production of bioethanol and biogas in which the peels were added to the fermentation residue prior to anaerobic digestion produced maximum fuel energy yield of (15-23MJ/kgVS).
25237774	6	44	theme	3-16	739:742	arg1	%					743:743	%	743:743	%	743:743	Starch content ranged from 77% to 81%, structural carbohydrates 3-16%, total crude protein ranged from 2% to 8%.
25237774	1	45	theme	bioenergy	103:111	arg1	carriers					113:120	bioenergy carriers	103:120	bioenergy carriers	103:120	Arg, for production of bioenergy carriers: Bioethanol and biogas.
25237774	5	46	theme	Composition	556:566	arg1	analysis					568:575	Composition analysis	556:575	Composition analysis	556:575	Composition analysis demonstrated that M. glaziovii is a suitable feedstock for both bioethanol and biogas production.
25237774	2	47	theme	study	168:172	arg1	objective					150:158	The objective	146:158	The objective of this study	146:172	The objective of this study was to characterise and evaluate a wild inedible cassava species, Manihot glaziovii as feedstock for bioenergy production.
25237774	2	48	theme	cassava	223:229	arg1	Manihot					240:246	Manihot	240:246	Manihot	240:246	The objective of this study was to characterise and evaluate a wild inedible cassava species, Manihot glaziovii as feedstock for bioenergy production.
25237774	2	48	theme	cassava	223:229	arg1	species					231:237	a wild inedible cassava species	207:237	a wild inedible cassava species	207:237	The objective of this study was to characterise and evaluate a wild inedible cassava species, Manihot glaziovii as feedstock for bioenergy production.
25237774	1	49	theme	carriers	113:120	arg1	production					89:98	production	89:98	production of bioenergy carriers	89:120	Arg, for production of bioenergy carriers: Bioethanol and biogas.
25237774	6	50	theme	2	778:778	arg1	%					779:779	%	779:779	%	779:779	Starch content ranged from 77% to 81%, structural carbohydrates 3-16%, total crude protein ranged from 2% to 8%.
25237774	2	51	theme	inedible	214:221	arg1	Manihot					240:246	Manihot	240:246	Manihot	240:246	The objective of this study was to characterise and evaluate a wild inedible cassava species, Manihot glaziovii as feedstock for bioenergy production.
25237774	2	51	theme	inedible	214:221	arg1	species					231:237	a wild inedible cassava species	207:237	a wild inedible cassava species	207:237	The objective of this study was to characterise and evaluate a wild inedible cassava species, Manihot glaziovii as feedstock for bioenergy production.
25237774	6	52	theme	structural	714:723	arg1	carbohydrates					725:737	structural carbohydrates	714:737	structural carbohydrates 3-16%	714:743	Starch content ranged from 77% to 81%, structural carbohydrates 3-16%, total crude protein ranged from 2% to 8%.
25237774	6	52	theme	structural	714:723	arg1	protein					758:764	total crude protein	746:764	total crude protein ranged from 2% to 8%	746:785	Starch content ranged from 77% to 81%, structural carbohydrates 3-16%, total crude protein ranged from 2% to 8%.
25237774	7	53	theme	fermentation	858:869	arg1	efficiency					871:880	a fermentation efficiency	856:880	a fermentation efficiency of 89%	856:887	Yeast fermentation achieved ethanol concentration of up to 85g/L at a fermentation efficiency of 89%.
25237774	0	54	theme	Manihot	54:60	arg1	feedstock					43:51	a novel feedstock	35:51	a novel feedstock	35:51	Characterisation and evaluation of a novel feedstock, Manihot glaziovii, Muell.
25237774	0	54	theme	Manihot	54:60	arg1	glaziovii					62:70	Manihot glaziovii	54:70	Manihot glaziovii	54:70	Characterisation and evaluation of a novel feedstock, Manihot glaziovii, Muell.
25237774	2	55	theme	wild	209:212	arg1	Manihot					240:246	Manihot	240:246	Manihot	240:246	The objective of this study was to characterise and evaluate a wild inedible cassava species, Manihot glaziovii as feedstock for bioenergy production.
25237774	2	55	theme	wild	209:212	arg1	species					231:237	a wild inedible cassava species	207:237	a wild inedible cassava species	207:237	The objective of this study was to characterise and evaluate a wild inedible cassava species, Manihot glaziovii as feedstock for bioenergy production.
25307474	10	0	theme	catechins	1554:1562	arg1	biotransformation					1533:1549	biotransformation	1533:1549	biotransformation of catechins with enhanced radical scavenging activity from green tea	1533:1619	CONCLUSION Simultaneous processing with tannase and Rapidase can be an efficient method for the extraction of bioactive polysaccharides and biotransformation of catechins with enhanced radical scavenging activity from green tea.
25307474	10	0	theme	catechins	1554:1562	arg1	processing					1417:1426	CONCLUSION Simultaneous processing	1393:1426	CONCLUSION Simultaneous processing with tannase and Rapidase	1393:1452	CONCLUSION Simultaneous processing with tannase and Rapidase can be an efficient method for the extraction of bioactive polysaccharides and biotransformation of catechins with enhanced radical scavenging activity from green tea.
25307474	10	0	theme	catechins	1554:1562	arg1	method					1474:1479	an efficient method	1461:1479	an efficient method for the extraction of bioactive polysaccharides	1461:1527	CONCLUSION Simultaneous processing with tannase and Rapidase can be an efficient method for the extraction of bioactive polysaccharides and biotransformation of catechins with enhanced radical scavenging activity from green tea.
25307474	8	1	theme	Interleukin	1164:1174	arg1	production					1185:1194	TPS-induced Interleukin 6 (IL-6) production	1152:1194	TPS-induced Interleukin 6 (IL-6) production in macrophages	1152:1209	In addition, pectinase-driven hydrolysis in polysaccharides significantly increased TPS-induced Interleukin 6 (IL-6) production in macrophages.
25307474	7	2	theme	Pectinase	976:984	arg1	treatment					986:994	Pectinase treatment	976:994	Pectinase treatment	976:994	Pectinase treatment led to change of TPS composition in GTE by hydrolyzing polysaccharides.
25307474	10	3	theme	polysaccharides	1513:1527	arg1	extraction					1489:1498	the extraction	1485:1498	the extraction of bioactive polysaccharides	1485:1527	CONCLUSION Simultaneous processing with tannase and Rapidase can be an efficient method for the extraction of bioactive polysaccharides and biotransformation of catechins with enhanced radical scavenging activity from green tea.
25307474	4	4	theme	catechins	606:614	arg1	biotransformation					585:601	the biotransformation	581:601	the biotransformation of catechins and altered tea polysaccharide () content	581:656	RESULTS Tannase and pectinase treatments induced the biotransformation of catechins and altered tea polysaccharide () content.
25307474	9	5	theme	TPS	1299:1301	arg1	samples					1303:1309	TPS samples	1299:1309	TPS samples	1299:1309	In particular, treatment of Rapidase (TPS-Ra) led to the highest IL-6 production among TPS samples, similar to treatment of highly purified pectinase (TPS-GTE), a positive control.
25307474	5	6	theme	GTE	675:677	arg1	addition					663:670	The addition	659:670	The addition of GTE to the enzyme reaction	659:700	The addition of GTE to the enzyme reaction resulted in a significant increase in degallated catechins, including gallic acid, a product of the tannase reaction (314.5-4076.0 µg mL(-1)) and a reduction in epigallocatechin gallate (EGCG).
25307474	4	7	theme	content	650:656	arg1	biotransformation					585:601	the biotransformation	581:601	the biotransformation of catechins and altered tea polysaccharide () content	581:656	RESULTS Tannase and pectinase treatments induced the biotransformation of catechins and altered tea polysaccharide () content.
25307474	10	8	with	processing	1417:1426	arg1	Rapidase					1445:1452	Rapidase	1445:1452	Rapidase	1445:1452	CONCLUSION Simultaneous processing with tannase and Rapidase can be an efficient method for the extraction of bioactive polysaccharides and biotransformation of catechins with enhanced radical scavenging activity from green tea.
25307474	10	8	with	processing	1417:1426	arg1	tannase					1433:1439	tannase	1433:1439	tannase	1433:1439	CONCLUSION Simultaneous processing with tannase and Rapidase can be an efficient method for the extraction of bioactive polysaccharides and biotransformation of catechins with enhanced radical scavenging activity from green tea.
25307474	0	9	theme	simultaneous	96:107	arg1	treatment					109:117	simultaneous treatment	96:117	simultaneous treatment with tannase and pectinase	96:144	Biotransformation of catechin and extraction of active polysaccharide from green tea leaves via simultaneous treatment with tannase and pectinase.
25307474	0	10	from	Biotransformation	0:16	arg1	tea					81:83	green tea	75:83	green tea	75:83	Biotransformation of catechin and extraction of active polysaccharide from green tea leaves via simultaneous treatment with tannase and pectinase.
25307474	5	11	dep	reaction	810:817	arg1	-1					839:840	-1	839:840	-1	839:840	The addition of GTE to the enzyme reaction resulted in a significant increase in degallated catechins, including gallic acid, a product of the tannase reaction (314.5-4076.0 µg mL(-1)) and a reduction in epigallocatechin gallate (EGCG).
25307474	5	11	dep	reaction	810:817	arg1	mL					836:837	314.5-4076.0 µg mL	820:837	314.5-4076.0 µg mL(-1)	820:841	The addition of GTE to the enzyme reaction resulted in a significant increase in degallated catechins, including gallic acid, a product of the tannase reaction (314.5-4076.0 µg mL(-1)) and a reduction in epigallocatechin gallate (EGCG).
25307474	4	12	theme	altered	620:626	arg1	content					650:656	altered tea polysaccharide () content	620:656	altered tea polysaccharide () content	620:656	RESULTS Tannase and pectinase treatments induced the biotransformation of catechins and altered tea polysaccharide () content.
25307474	3	13	from	effect	376:381	arg1	biotransformation					438:454	biotransformation	438:454	biotransformation of catechins and extraction of polysaccharide from green tea extract (GTE)	438:529	In this study, we investigated the effect of simultaneous treatment with tannase and Rapidase on biotransformation of catechins and extraction of polysaccharide from green tea extract (GTE).
25307474	9	14	theme	Rapidase	1240:1247	arg1	treatment					1227:1235	treatment	1227:1235	treatment of Rapidase (TPS-Ra)	1227:1256	In particular, treatment of Rapidase (TPS-Ra) led to the highest IL-6 production among TPS samples, similar to treatment of highly purified pectinase (TPS-GTE), a positive control.
25307474	10	15	theme	scavenging	1586:1595	arg1	activity					1597:1604	enhanced radical scavenging activity	1569:1604	enhanced radical scavenging activity from green tea	1569:1619	CONCLUSION Simultaneous processing with tannase and Rapidase can be an efficient method for the extraction of bioactive polysaccharides and biotransformation of catechins with enhanced radical scavenging activity from green tea.
25307474	3	16	theme	polysaccharide	487:500	arg1	extraction					473:482	extraction	473:482	extraction of polysaccharide from green tea extract (GTE)	473:529	In this study, we investigated the effect of simultaneous treatment with tannase and Rapidase on biotransformation of catechins and extraction of polysaccharide from green tea extract (GTE).
25307474	3	16	theme	polysaccharide	487:500	arg1	catechins					459:467	catechins	459:467	catechins	459:467	In this study, we investigated the effect of simultaneous treatment with tannase and Rapidase on biotransformation of catechins and extraction of polysaccharide from green tea extract (GTE).
25307474	2	17	theme	components	288:297	arg1	bioconversion					261:273	the bioconversion	257:273	the bioconversion of bioactive components, which can improve biological activities	257:338	Enzymatic treatments induce the bioconversion of bioactive components, which can improve biological activities.
25307474	5	18	theme	tannase	802:808	arg1	reaction					810:817	the tannase reaction	798:817	the tannase reaction (314.5-4076.0 µg mL(-1))	798:842	The addition of GTE to the enzyme reaction resulted in a significant increase in degallated catechins, including gallic acid, a product of the tannase reaction (314.5-4076.0 µg mL(-1)) and a reduction in epigallocatechin gallate (EGCG).
25307474	8	19	from	hydrolysis	1098:1107	arg1	polysaccharides					1112:1126	polysaccharides	1112:1126	polysaccharides	1112:1126	In addition, pectinase-driven hydrolysis in polysaccharides significantly increased TPS-induced Interleukin 6 (IL-6) production in macrophages.
25307474	5	20	theme	reaction	810:817	arg1	acid					779:782	gallic acid	772:782	gallic acid	772:782	The addition of GTE to the enzyme reaction resulted in a significant increase in degallated catechins, including gallic acid, a product of the tannase reaction (314.5-4076.0 µg mL(-1)) and a reduction in epigallocatechin gallate (EGCG).
25307474	5	20	theme	reaction	810:817	arg1	product					787:793	a product	785:793	a product of the tannase reaction (314.5-4076.0 µg mL(-1))	785:842	The addition of GTE to the enzyme reaction resulted in a significant increase in degallated catechins, including gallic acid, a product of the tannase reaction (314.5-4076.0 µg mL(-1)) and a reduction in epigallocatechin gallate (EGCG).
25307474	2	21	theme	bioactive	278:286	arg1	components					288:297	bioactive components	278:297	bioactive components	278:297	Enzymatic treatments induce the bioconversion of bioactive components, which can improve biological activities.
25307474	10	22	theme	enhanced	1569:1576	arg1	activity					1597:1604	enhanced radical scavenging activity	1569:1604	enhanced radical scavenging activity from green tea	1569:1619	CONCLUSION Simultaneous processing with tannase and Rapidase can be an efficient method for the extraction of bioactive polysaccharides and biotransformation of catechins with enhanced radical scavenging activity from green tea.
25307474	3	23	theme	catechins	459:467	arg1	biotransformation					438:454	biotransformation	438:454	biotransformation of catechins and extraction of polysaccharide from green tea extract (GTE)	438:529	In this study, we investigated the effect of simultaneous treatment with tannase and Rapidase on biotransformation of catechins and extraction of polysaccharide from green tea extract (GTE).
25307474	3	24	theme	simultaneous	386:397	arg1	treatment					399:407	simultaneous treatment	386:407	simultaneous treatment with tannase and Rapidase	386:433	In this study, we investigated the effect of simultaneous treatment with tannase and Rapidase on biotransformation of catechins and extraction of polysaccharide from green tea extract (GTE).
25307474	9	25	theme	pectinase	1352:1360	arg1	treatment					1323:1331	treatment	1323:1331	treatment of highly purified pectinase (TPS-GTE), a positive control	1323:1390	In particular, treatment of Rapidase (TPS-Ra) led to the highest IL-6 production among TPS samples, similar to treatment of highly purified pectinase (TPS-GTE), a positive control.
25307474	0	26	with	treatment	109:117	arg1	pectinase					136:144	pectinase	136:144	pectinase	136:144	Biotransformation of catechin and extraction of active polysaccharide from green tea leaves via simultaneous treatment with tannase and pectinase.
25307474	0	26	with	treatment	109:117	arg1	tannase					124:130	tannase	124:130	tannase	124:130	Biotransformation of catechin and extraction of active polysaccharide from green tea leaves via simultaneous treatment with tannase and pectinase.
25307474	3	27	theme	treatment	399:407	arg1	effect					376:381	the effect	372:381	the effect of simultaneous treatment with tannase and Rapidase on biotransformation of catechins and extraction of polysaccharide from green tea extract (GTE)	372:529	In this study, we investigated the effect of simultaneous treatment with tannase and Rapidase on biotransformation of catechins and extraction of polysaccharide from green tea extract (GTE).
25307474	6	28	theme	GTE	971:973	arg1	activity					959:966	the radical scavenging activity	936:966	the radical scavenging activity of GTE	936:973	Biotransformation of catechins improved the radical scavenging activity of GTE.
25307474	9	29	theme	IL-6	1277:1280	arg1	production					1282:1291	the highest IL-6 production	1265:1291	the highest IL-6 production among TPS samples	1265:1309	In particular, treatment of Rapidase (TPS-Ra) led to the highest IL-6 production among TPS samples, similar to treatment of highly purified pectinase (TPS-GTE), a positive control.
25307474	4	30	dep	RESULTS	532:538	arg1	Tannase					540:546	Tannase	540:546	Tannase	540:546	RESULTS Tannase and pectinase treatments induced the biotransformation of catechins and altered tea polysaccharide () content.
25307474	4	30	dep	RESULTS	532:538	arg1	treatments					562:571	pectinase treatments	552:571	pectinase treatments	552:571	RESULTS Tannase and pectinase treatments induced the biotransformation of catechins and altered tea polysaccharide () content.
25307474	2	31	theme	biological	318:327	arg1	activities					329:338	biological activities	318:338	biological activities	318:338	Enzymatic treatments induce the bioconversion of bioactive components, which can improve biological activities.
25307474	5	32	theme	degallated	740:749	arg1	acid					779:782	gallic acid	772:782	gallic acid	772:782	The addition of GTE to the enzyme reaction resulted in a significant increase in degallated catechins, including gallic acid, a product of the tannase reaction (314.5-4076.0 µg mL(-1)) and a reduction in epigallocatechin gallate (EGCG).
25307474	5	32	theme	degallated	740:749	arg1	catechins					751:759	degallated catechins	740:759	degallated catechins	740:759	The addition of GTE to the enzyme reaction resulted in a significant increase in degallated catechins, including gallic acid, a product of the tannase reaction (314.5-4076.0 µg mL(-1)) and a reduction in epigallocatechin gallate (EGCG).
25307474	10	33	theme	Simultaneous	1404:1415	arg1	processing					1417:1426	CONCLUSION Simultaneous processing	1393:1426	CONCLUSION Simultaneous processing with tannase and Rapidase	1393:1452	CONCLUSION Simultaneous processing with tannase and Rapidase can be an efficient method for the extraction of bioactive polysaccharides and biotransformation of catechins with enhanced radical scavenging activity from green tea.
25307474	10	33	theme	Simultaneous	1404:1415	arg1	method					1474:1479	an efficient method	1461:1479	an efficient method for the extraction of bioactive polysaccharides	1461:1527	CONCLUSION Simultaneous processing with tannase and Rapidase can be an efficient method for the extraction of bioactive polysaccharides and biotransformation of catechins with enhanced radical scavenging activity from green tea.
25307474	3	34	from	extract	517:523	arg1	extraction					473:482	extraction	473:482	extraction of polysaccharide from green tea extract (GTE)	473:529	In this study, we investigated the effect of simultaneous treatment with tannase and Rapidase on biotransformation of catechins and extraction of polysaccharide from green tea extract (GTE).
25307474	3	34	from	extract	517:523	arg1	catechins					459:467	catechins	459:467	catechins	459:467	In this study, we investigated the effect of simultaneous treatment with tannase and Rapidase on biotransformation of catechins and extraction of polysaccharide from green tea extract (GTE).
25307474	3	34	from	extract	517:523	arg1	biotransformation					438:454	biotransformation	438:454	biotransformation of catechins and extraction of polysaccharide from green tea extract (GTE)	438:529	In this study, we investigated the effect of simultaneous treatment with tannase and Rapidase on biotransformation of catechins and extraction of polysaccharide from green tea extract (GTE).
25307474	10	35	with	catechins	1554:1562	arg1	activity					1597:1604	enhanced radical scavenging activity	1569:1604	enhanced radical scavenging activity from green tea	1569:1619	CONCLUSION Simultaneous processing with tannase and Rapidase can be an efficient method for the extraction of bioactive polysaccharides and biotransformation of catechins with enhanced radical scavenging activity from green tea.
25307474	1	36	theme	bioactive	191:199	arg1	compounds					201:209	bioactive compounds	191:209	bioactive compounds	191:209	BACKGROUND Green tea is a dietary source of bioactive compounds for human health.
25307474	0	37	theme	catechin	21:28	arg1	Biotransformation					0:16	Biotransformation	0:16	Biotransformation of catechin and extraction of active polysaccharide from green tea	0:83	Biotransformation of catechin and extraction of active polysaccharide from green tea leaves via simultaneous treatment with tannase and pectinase.
25307474	9	38	theme	purified	1343:1350	arg1	control					1384:1390	a positive control	1373:1390	a positive control	1373:1390	In particular, treatment of Rapidase (TPS-Ra) led to the highest IL-6 production among TPS samples, similar to treatment of highly purified pectinase (TPS-GTE), a positive control.
25307474	9	38	theme	purified	1343:1350	arg1	TPS-GTE					1363:1369	TPS-GTE	1363:1369	TPS-GTE	1363:1369	In particular, treatment of Rapidase (TPS-Ra) led to the highest IL-6 production among TPS samples, similar to treatment of highly purified pectinase (TPS-GTE), a positive control.
25307474	9	38	theme	purified	1343:1350	arg1	pectinase					1352:1360	highly purified pectinase	1336:1360	highly purified pectinase (TPS-GTE)	1336:1370	In particular, treatment of Rapidase (TPS-Ra) led to the highest IL-6 production among TPS samples, similar to treatment of highly purified pectinase (TPS-GTE), a positive control.
25307474	5	39	theme	significant	716:726	arg1	increase					728:735	a significant increase	714:735	a significant increase in degallated catechins, including gallic acid, a product of the tannase reaction (314.5-4076.0 µg mL(-1))	714:842	The addition of GTE to the enzyme reaction resulted in a significant increase in degallated catechins, including gallic acid, a product of the tannase reaction (314.5-4076.0 µg mL(-1)) and a reduction in epigallocatechin gallate (EGCG).
25307474	3	40	with	treatment	399:407	arg1	tannase					414:420	tannase	414:420	tannase	414:420	In this study, we investigated the effect of simultaneous treatment with tannase and Rapidase on biotransformation of catechins and extraction of polysaccharide from green tea extract (GTE).
25307474	3	40	with	treatment	399:407	arg1	Rapidase					426:433	Rapidase	426:433	Rapidase	426:433	In this study, we investigated the effect of simultaneous treatment with tannase and Rapidase on biotransformation of catechins and extraction of polysaccharide from green tea extract (GTE).
25307474	1	41	theme	compounds	201:209	arg1	source					181:186	a dietary source	171:186	a dietary source of bioactive compounds for human health	171:226	BACKGROUND Green tea is a dietary source of bioactive compounds for human health.
25307474	1	41	theme	compounds	201:209	arg1	tea					164:166	BACKGROUND Green tea	147:166	BACKGROUND Green tea	147:166	BACKGROUND Green tea is a dietary source of bioactive compounds for human health.
25307474	7	42	from	change	1003:1008	arg1	GTE					1032:1034	GTE	1032:1034	GTE by hydrolyzing polysaccharides	1032:1065	Pectinase treatment led to change of TPS composition in GTE by hydrolyzing polysaccharides.
25307474	6	43	theme	scavenging	948:957	arg1	activity					959:966	the radical scavenging activity	936:966	the radical scavenging activity of GTE	936:973	Biotransformation of catechins improved the radical scavenging activity of GTE.
25307474	7	44	theme	hydrolyzing	1039:1049	arg1	polysaccharides					1051:1065	hydrolyzing polysaccharides	1039:1065	hydrolyzing polysaccharides	1039:1065	Pectinase treatment led to change of TPS composition in GTE by hydrolyzing polysaccharides.
25307474	0	45	from	tea	81:83	arg1	Biotransformation					0:16	Biotransformation	0:16	Biotransformation of catechin and extraction of active polysaccharide from green tea	0:83	Biotransformation of catechin and extraction of active polysaccharide from green tea leaves via simultaneous treatment with tannase and pectinase.
25307474	0	45	from	tea	81:83	arg1	polysaccharide					55:68	active polysaccharide	48:68	active polysaccharide from green tea	48:83	Biotransformation of catechin and extraction of active polysaccharide from green tea leaves via simultaneous treatment with tannase and pectinase.
25307474	0	46	theme	extraction	34:43	arg1	Biotransformation					0:16	Biotransformation	0:16	Biotransformation of catechin and extraction of active polysaccharide from green tea	0:83	Biotransformation of catechin and extraction of active polysaccharide from green tea leaves via simultaneous treatment with tannase and pectinase.
25307474	6	47	theme	radical	940:946	arg1	activity					959:966	the radical scavenging activity	936:966	the radical scavenging activity of GTE	936:973	Biotransformation of catechins improved the radical scavenging activity of GTE.
25307474	8	48	theme	pectinase-driven	1081:1096	arg1	hydrolysis					1098:1107	pectinase-driven hydrolysis	1081:1107	pectinase-driven hydrolysis in polysaccharides	1081:1126	In addition, pectinase-driven hydrolysis in polysaccharides significantly increased TPS-induced Interleukin 6 (IL-6) production in macrophages.
25307474	2	49	theme	Enzymatic	229:237	arg1	treatments					239:248	Enzymatic treatments	229:248	Enzymatic treatments	229:248	Enzymatic treatments induce the bioconversion of bioactive components, which can improve biological activities.
25307474	5	50	from	increase	728:735	arg1	acid					779:782	gallic acid	772:782	gallic acid	772:782	The addition of GTE to the enzyme reaction resulted in a significant increase in degallated catechins, including gallic acid, a product of the tannase reaction (314.5-4076.0 µg mL(-1)) and a reduction in epigallocatechin gallate (EGCG).
25307474	5	50	from	increase	728:735	arg1	EGCG					889:892	EGCG	889:892	EGCG	889:892	The addition of GTE to the enzyme reaction resulted in a significant increase in degallated catechins, including gallic acid, a product of the tannase reaction (314.5-4076.0 µg mL(-1)) and a reduction in epigallocatechin gallate (EGCG).
25307474	5	50	from	increase	728:735	arg1	catechins					751:759	degallated catechins	740:759	degallated catechins	740:759	The addition of GTE to the enzyme reaction resulted in a significant increase in degallated catechins, including gallic acid, a product of the tannase reaction (314.5-4076.0 µg mL(-1)) and a reduction in epigallocatechin gallate (EGCG).
25307474	5	50	from	increase	728:735	arg1	gallate					880:886	epigallocatechin gallate	863:886	epigallocatechin gallate (EGCG)	863:893	The addition of GTE to the enzyme reaction resulted in a significant increase in degallated catechins, including gallic acid, a product of the tannase reaction (314.5-4076.0 µg mL(-1)) and a reduction in epigallocatechin gallate (EGCG).
25307474	1	51	theme	human	215:219	arg1	health					221:226	human health	215:226	human health	215:226	BACKGROUND Green tea is a dietary source of bioactive compounds for human health.
25307474	0	52	theme	polysaccharide	55:68	arg1	Biotransformation					0:16	Biotransformation	0:16	Biotransformation of catechin and extraction of active polysaccharide from green tea	0:83	Biotransformation of catechin and extraction of active polysaccharide from green tea leaves via simultaneous treatment with tannase and pectinase.
25307474	5	53	theme	epigallocatechin	863:878	arg1	EGCG					889:892	EGCG	889:892	EGCG	889:892	The addition of GTE to the enzyme reaction resulted in a significant increase in degallated catechins, including gallic acid, a product of the tannase reaction (314.5-4076.0 µg mL(-1)) and a reduction in epigallocatechin gallate (EGCG).
25307474	5	53	theme	epigallocatechin	863:878	arg1	gallate					880:886	epigallocatechin gallate	863:886	epigallocatechin gallate (EGCG)	863:893	The addition of GTE to the enzyme reaction resulted in a significant increase in degallated catechins, including gallic acid, a product of the tannase reaction (314.5-4076.0 µg mL(-1)) and a reduction in epigallocatechin gallate (EGCG).
25307474	5	54	theme	enzyme	686:691	arg1	reaction					693:700	the enzyme reaction	682:700	the enzyme reaction	682:700	The addition of GTE to the enzyme reaction resulted in a significant increase in degallated catechins, including gallic acid, a product of the tannase reaction (314.5-4076.0 µg mL(-1)) and a reduction in epigallocatechin gallate (EGCG).
25307474	7	55	theme	TPS	1013:1015	arg1	composition					1017:1027	TPS composition	1013:1027	TPS composition	1013:1027	Pectinase treatment led to change of TPS composition in GTE by hydrolyzing polysaccharides.
25307474	3	56	theme	tea	513:515	arg1	GTE					526:528	GTE	526:528	GTE	526:528	In this study, we investigated the effect of simultaneous treatment with tannase and Rapidase on biotransformation of catechins and extraction of polysaccharide from green tea extract (GTE).
25307474	3	56	theme	tea	513:515	arg1	extract					517:523	green tea extract	507:523	green tea extract (GTE)	507:529	In this study, we investigated the effect of simultaneous treatment with tannase and Rapidase on biotransformation of catechins and extraction of polysaccharide from green tea extract (GTE).
25307474	0	57	theme	active	48:53	arg1	polysaccharide					55:68	active polysaccharide	48:68	active polysaccharide from green tea	48:83	Biotransformation of catechin and extraction of active polysaccharide from green tea leaves via simultaneous treatment with tannase and pectinase.
25307474	4	58	theme	pectinase	552:560	arg1	treatments					562:571	pectinase treatments	552:571	pectinase treatments	552:571	RESULTS Tannase and pectinase treatments induced the biotransformation of catechins and altered tea polysaccharide () content.
25307474	9	59	theme	positive	1375:1382	arg1	control					1384:1390	a positive control	1373:1390	a positive control	1373:1390	In particular, treatment of Rapidase (TPS-Ra) led to the highest IL-6 production among TPS samples, similar to treatment of highly purified pectinase (TPS-GTE), a positive control.
25307474	9	59	theme	positive	1375:1382	arg1	pectinase					1352:1360	highly purified pectinase	1336:1360	highly purified pectinase (TPS-GTE)	1336:1370	In particular, treatment of Rapidase (TPS-Ra) led to the highest IL-6 production among TPS samples, similar to treatment of highly purified pectinase (TPS-GTE), a positive control.
25307474	10	60	theme	CONCLUSION	1393:1402	arg1	processing					1417:1426	CONCLUSION Simultaneous processing	1393:1426	CONCLUSION Simultaneous processing with tannase and Rapidase	1393:1452	CONCLUSION Simultaneous processing with tannase and Rapidase can be an efficient method for the extraction of bioactive polysaccharides and biotransformation of catechins with enhanced radical scavenging activity from green tea.
25307474	10	60	theme	CONCLUSION	1393:1402	arg1	method					1474:1479	an efficient method	1461:1479	an efficient method for the extraction of bioactive polysaccharides	1461:1527	CONCLUSION Simultaneous processing with tannase and Rapidase can be an efficient method for the extraction of bioactive polysaccharides and biotransformation of catechins with enhanced radical scavenging activity from green tea.
25307474	10	61	theme	radical	1578:1584	arg1	activity					1597:1604	enhanced radical scavenging activity	1569:1604	enhanced radical scavenging activity from green tea	1569:1619	CONCLUSION Simultaneous processing with tannase and Rapidase can be an efficient method for the extraction of bioactive polysaccharides and biotransformation of catechins with enhanced radical scavenging activity from green tea.
25307474	10	62	theme	efficient	1464:1472	arg1	processing					1417:1426	CONCLUSION Simultaneous processing	1393:1426	CONCLUSION Simultaneous processing with tannase and Rapidase	1393:1452	CONCLUSION Simultaneous processing with tannase and Rapidase can be an efficient method for the extraction of bioactive polysaccharides and biotransformation of catechins with enhanced radical scavenging activity from green tea.
25307474	10	62	theme	efficient	1464:1472	arg1	method					1474:1479	an efficient method	1461:1479	an efficient method for the extraction of bioactive polysaccharides	1461:1527	CONCLUSION Simultaneous processing with tannase and Rapidase can be an efficient method for the extraction of bioactive polysaccharides and biotransformation of catechins with enhanced radical scavenging activity from green tea.
25307474	6	63	theme	catechins	917:925	arg1	Biotransformation					896:912	Biotransformation	896:912	Biotransformation of catechins	896:925	Biotransformation of catechins improved the radical scavenging activity of GTE.
25307474	7	64	theme	composition	1017:1027	arg1	change					1003:1008	change	1003:1008	change of TPS composition in GTE by hydrolyzing polysaccharides	1003:1065	Pectinase treatment led to change of TPS composition in GTE by hydrolyzing polysaccharides.
25307474	8	65	from	production	1185:1194	arg1	macrophages					1199:1209	macrophages	1199:1209	macrophages	1199:1209	In addition, pectinase-driven hydrolysis in polysaccharides significantly increased TPS-induced Interleukin 6 (IL-6) production in macrophages.
25307474	4	66	theme	polysaccharide	632:645	arg1	content					650:656	altered tea polysaccharide () content	620:656	altered tea polysaccharide () content	620:656	RESULTS Tannase and pectinase treatments induced the biotransformation of catechins and altered tea polysaccharide () content.
25307474	10	67	theme	bioactive	1503:1511	arg1	polysaccharides					1513:1527	bioactive polysaccharides	1503:1527	bioactive polysaccharides	1503:1527	CONCLUSION Simultaneous processing with tannase and Rapidase can be an efficient method for the extraction of bioactive polysaccharides and biotransformation of catechins with enhanced radical scavenging activity from green tea.
25307474	9	68	theme	highest	1269:1275	arg1	production					1282:1291	the highest IL-6 production	1265:1291	the highest IL-6 production among TPS samples	1265:1309	In particular, treatment of Rapidase (TPS-Ra) led to the highest IL-6 production among TPS samples, similar to treatment of highly purified pectinase (TPS-GTE), a positive control.
25307474	5	69	from	reduction	850:858	arg1	acid					779:782	gallic acid	772:782	gallic acid	772:782	The addition of GTE to the enzyme reaction resulted in a significant increase in degallated catechins, including gallic acid, a product of the tannase reaction (314.5-4076.0 µg mL(-1)) and a reduction in epigallocatechin gallate (EGCG).
25307474	5	69	from	reduction	850:858	arg1	EGCG					889:892	EGCG	889:892	EGCG	889:892	The addition of GTE to the enzyme reaction resulted in a significant increase in degallated catechins, including gallic acid, a product of the tannase reaction (314.5-4076.0 µg mL(-1)) and a reduction in epigallocatechin gallate (EGCG).
25307474	5	69	from	reduction	850:858	arg1	catechins					751:759	degallated catechins	740:759	degallated catechins	740:759	The addition of GTE to the enzyme reaction resulted in a significant increase in degallated catechins, including gallic acid, a product of the tannase reaction (314.5-4076.0 µg mL(-1)) and a reduction in epigallocatechin gallate (EGCG).
25307474	5	69	from	reduction	850:858	arg1	gallate					880:886	epigallocatechin gallate	863:886	epigallocatechin gallate (EGCG)	863:893	The addition of GTE to the enzyme reaction resulted in a significant increase in degallated catechins, including gallic acid, a product of the tannase reaction (314.5-4076.0 µg mL(-1)) and a reduction in epigallocatechin gallate (EGCG).
25307474	3	70	theme	green	507:511	arg1	GTE					526:528	GTE	526:528	GTE	526:528	In this study, we investigated the effect of simultaneous treatment with tannase and Rapidase on biotransformation of catechins and extraction of polysaccharide from green tea extract (GTE).
25307474	3	70	theme	green	507:511	arg1	extract					517:523	green tea extract	507:523	green tea extract (GTE)	507:529	In this study, we investigated the effect of simultaneous treatment with tannase and Rapidase on biotransformation of catechins and extraction of polysaccharide from green tea extract (GTE).
25307474	4	71	theme	tea	628:630	arg1	content					650:656	altered tea polysaccharide () content	620:656	altered tea polysaccharide () content	620:656	RESULTS Tannase and pectinase treatments induced the biotransformation of catechins and altered tea polysaccharide () content.
25307474	5	72	theme	gallic	772:777	arg1	acid					779:782	gallic acid	772:782	gallic acid	772:782	The addition of GTE to the enzyme reaction resulted in a significant increase in degallated catechins, including gallic acid, a product of the tannase reaction (314.5-4076.0 µg mL(-1)) and a reduction in epigallocatechin gallate (EGCG).
25307474	5	72	theme	gallic	772:777	arg1	product					787:793	a product	785:793	a product of the tannase reaction (314.5-4076.0 µg mL(-1))	785:842	The addition of GTE to the enzyme reaction resulted in a significant increase in degallated catechins, including gallic acid, a product of the tannase reaction (314.5-4076.0 µg mL(-1)) and a reduction in epigallocatechin gallate (EGCG).
25307474	3	73	theme	extraction	473:482	arg1	biotransformation					438:454	biotransformation	438:454	biotransformation of catechins and extraction of polysaccharide from green tea extract (GTE)	438:529	In this study, we investigated the effect of simultaneous treatment with tannase and Rapidase on biotransformation of catechins and extraction of polysaccharide from green tea extract (GTE).
25307474	3	74	from	biotransformation	438:454	arg1	GTE					526:528	GTE	526:528	GTE	526:528	In this study, we investigated the effect of simultaneous treatment with tannase and Rapidase on biotransformation of catechins and extraction of polysaccharide from green tea extract (GTE).
25307474	3	74	from	biotransformation	438:454	arg1	extract					517:523	green tea extract	507:523	green tea extract (GTE)	507:529	In this study, we investigated the effect of simultaneous treatment with tannase and Rapidase on biotransformation of catechins and extraction of polysaccharide from green tea extract (GTE).
25307474	1	75	theme	BACKGROUND	147:156	arg1	source					181:186	a dietary source	171:186	a dietary source of bioactive compounds for human health	171:226	BACKGROUND Green tea is a dietary source of bioactive compounds for human health.
25307474	1	75	theme	BACKGROUND	147:156	arg1	tea					164:166	BACKGROUND Green tea	147:166	BACKGROUND Green tea	147:166	BACKGROUND Green tea is a dietary source of bioactive compounds for human health.
25307474	1	76	theme	dietary	173:179	arg1	source					181:186	a dietary source	171:186	a dietary source of bioactive compounds for human health	171:226	BACKGROUND Green tea is a dietary source of bioactive compounds for human health.
25307474	1	76	theme	dietary	173:179	arg1	tea					164:166	BACKGROUND Green tea	147:166	BACKGROUND Green tea	147:166	BACKGROUND Green tea is a dietary source of bioactive compounds for human health.
25307474	8	77	theme	TPS-induced	1152:1162	arg1	production					1185:1194	TPS-induced Interleukin 6 (IL-6) production	1152:1194	TPS-induced Interleukin 6 (IL-6) production in macrophages	1152:1209	In addition, pectinase-driven hydrolysis in polysaccharides significantly increased TPS-induced Interleukin 6 (IL-6) production in macrophages.
25307474	10	78	from	tea	1617:1619	arg1	activity					1597:1604	enhanced radical scavenging activity	1569:1604	enhanced radical scavenging activity from green tea	1569:1619	CONCLUSION Simultaneous processing with tannase and Rapidase can be an efficient method for the extraction of bioactive polysaccharides and biotransformation of catechins with enhanced radical scavenging activity from green tea.
25307474	1	79	theme	Green	158:162	arg1	source					181:186	a dietary source	171:186	a dietary source of bioactive compounds for human health	171:226	BACKGROUND Green tea is a dietary source of bioactive compounds for human health.
25307474	1	79	theme	Green	158:162	arg1	tea					164:166	BACKGROUND Green tea	147:166	BACKGROUND Green tea	147:166	BACKGROUND Green tea is a dietary source of bioactive compounds for human health.
25390070	9	0	theme	chiral	1745:1750	arg1	phase					1760:1764	the initial chiral nematic phase	1733:1764	the initial chiral nematic phase	1733:1764	The higher modulus (in compression) was detected for a composite reinforced by locking-in the uniaxial CNC alignment attainable through conversion of the initial chiral nematic phase into a nematic phase in the rotational magnetic field.
25390070	1	1	from	suspensions	247:257	arg1	methacrylate					292:303	aqueous 2-hydroxyethyl methacrylate	269:303	aqueous 2-hydroxyethyl methacrylate (HEMA)	269:310	Novel polymer composites reinforced with an oriented cellulose nanocrystal (CNC) assembly were prepared from suspensions of CNC in aqueous 2-hydroxyethyl methacrylate (HEMA) via magnetic field application to the suspensions followed by polymerization treatment.
25390070	1	1	from	suspensions	247:257	arg1	HEMA					306:309	HEMA	306:309	HEMA	306:309	Novel polymer composites reinforced with an oriented cellulose nanocrystal (CNC) assembly were prepared from suspensions of CNC in aqueous 2-hydroxyethyl methacrylate (HEMA) via magnetic field application to the suspensions followed by polymerization treatment.
25390070	9	2	theme	phase	1760:1764	arg1	conversion					1719:1728	conversion	1719:1728	conversion of the initial chiral nematic phase into a nematic phase in the rotational magnetic field	1719:1818	The higher modulus (in compression) was detected for a composite reinforced by locking-in the uniaxial CNC alignment attainable through conversion of the initial chiral nematic phase into a nematic phase in the rotational magnetic field.
25390070	9	3	from	modulus	1594:1600	arg1	compression					1606:1616	compression	1606:1616	compression	1606:1616	The higher modulus (in compression) was detected for a composite reinforced by locking-in the uniaxial CNC alignment attainable through conversion of the initial chiral nematic phase into a nematic phase in the rotational magnetic field.
25390070	4	4	theme	HEMA	696:699	arg1	polymerization					678:691	UV-induced polymerization	667:691	UV-induced polymerization of HEMA	667:699	UV-induced polymerization of HEMA perpetuated the respective states of magnetic orientation invested for the CNC dispersions to yield variously oriented CNC/poly(2-hydroxyethyl methacrylate) composites.
25390070	3	5	theme	rotational	579:588	arg1	field					599:603	A static or rotational magnetic field	567:603	A static or rotational magnetic field	567:603	A static or rotational magnetic field was applied to the isolated isotropic and anisotropic phases.
25390070	3	6	theme	static	569:574	arg1	field					599:603	A static or rotational magnetic field	567:603	A static or rotational magnetic field	567:603	A static or rotational magnetic field was applied to the isolated isotropic and anisotropic phases.
25390070	2	7	theme	quiescent	547:555	arg1	standing					557:564	quiescent standing	547:564	quiescent standing	547:564	The starting suspensions used at ∼6 wt % CNC separated into an upper isotropic phase and a lower anisotropic (chiral nematic) one in the course of quiescent standing.
25390070	1	8	from	methacrylate	292:303	arg1	suspensions					247:257	suspensions	247:257	suspensions of CNC in aqueous 2-hydroxyethyl methacrylate (HEMA)	247:310	Novel polymer composites reinforced with an oriented cellulose nanocrystal (CNC) assembly were prepared from suspensions of CNC in aqueous 2-hydroxyethyl methacrylate (HEMA) via magnetic field application to the suspensions followed by polymerization treatment.
25390070	9	9	theme	magnetic	1805:1812	arg1	field					1814:1818	the rotational magnetic field	1790:1818	the rotational magnetic field	1790:1818	The higher modulus (in compression) was detected for a composite reinforced by locking-in the uniaxial CNC alignment attainable through conversion of the initial chiral nematic phase into a nematic phase in the rotational magnetic field.
25390070	4	10	theme	magnetic	738:745	arg1	orientation					747:757	magnetic orientation	738:757	magnetic orientation invested for the CNC dispersions	738:790	UV-induced polymerization of HEMA perpetuated the respective states of magnetic orientation invested for the CNC dispersions to yield variously oriented CNC/poly(2-hydroxyethyl methacrylate) composites.
25390070	2	11	from	one	526:528	arg1	course					537:542	the course	533:542	the course of quiescent standing	533:564	The starting suspensions used at ∼6 wt % CNC separated into an upper isotropic phase and a lower anisotropic (chiral nematic) one in the course of quiescent standing.
25390070	5	12	theme	scanning	965:972	arg1	microscopy					983:992	optical and scanning electron microscopy	953:992	microscopy	983:992	The structural characterization was carried out by use of X-ray diffractometry and optical and scanning electron microscopy.
25390070	8	13	theme	static	1547:1552	arg1	magnetization					1568:1580	static or rotational magnetization	1547:1580	static or rotational magnetization	1547:1580	In dynamic mechanical experiments in tensile or compressive mode, we observed a clear mechanical anisotropy for the polymer composites synthesized from wholly anisotropic suspensions under static or rotational magnetization.
25390070	8	14	theme	dynamic	1361:1367	arg1	experiments					1380:1390	dynamic mechanical experiments	1361:1390	dynamic mechanical experiments in tensile or compressive mode	1361:1421	In dynamic mechanical experiments in tensile or compressive mode, we observed a clear mechanical anisotropy for the polymer composites synthesized from wholly anisotropic suspensions under static or rotational magnetization.
25390070	1	15	from	CNC	262:264	arg1	methacrylate					292:303	aqueous 2-hydroxyethyl methacrylate	269:303	aqueous 2-hydroxyethyl methacrylate (HEMA)	269:310	Novel polymer composites reinforced with an oriented cellulose nanocrystal (CNC) assembly were prepared from suspensions of CNC in aqueous 2-hydroxyethyl methacrylate (HEMA) via magnetic field application to the suspensions followed by polymerization treatment.
25390070	1	15	from	CNC	262:264	arg1	HEMA					306:309	HEMA	306:309	HEMA	306:309	Novel polymer composites reinforced with an oriented cellulose nanocrystal (CNC) assembly were prepared from suspensions of CNC in aqueous 2-hydroxyethyl methacrylate (HEMA) via magnetic field application to the suspensions followed by polymerization treatment.
25390070	3	16	theme	isotropic	633:641	arg1	phases					659:664	the isolated isotropic and anisotropic phases	620:664	the isolated isotropic and anisotropic phases	620:664	A static or rotational magnetic field was applied to the isolated isotropic and anisotropic phases.
25390070	5	17	theme	microscopy	983:992	arg1	use					921:923	use	921:923	use of X-ray diffractometry and optical and scanning electron microscopy	921:992	The structural characterization was carried out by use of X-ray diffractometry and optical and scanning electron microscopy.
25390070	9	18	theme	higher	1587:1592	arg1	modulus					1594:1600	The higher modulus	1583:1600	The higher modulus (in compression)	1583:1617	The higher modulus (in compression) was detected for a composite reinforced by locking-in the uniaxial CNC alignment attainable through conversion of the initial chiral nematic phase into a nematic phase in the rotational magnetic field.
25390070	8	19	theme	rotational	1557:1566	arg1	magnetization					1568:1580	static or rotational magnetization	1547:1580	static or rotational magnetization	1547:1580	In dynamic mechanical experiments in tensile or compressive mode, we observed a clear mechanical anisotropy for the polymer composites synthesized from wholly anisotropic suspensions under static or rotational magnetization.
25390070	5	20	theme	diffractometry	934:947	arg1	use					921:923	use	921:923	use of X-ray diffractometry and optical and scanning electron microscopy	921:992	The structural characterization was carried out by use of X-ray diffractometry and optical and scanning electron microscopy.
25390070	2	21	theme	chiral	510:515	arg1	nematic					517:523	chiral nematic	510:523	chiral nematic	510:523	The starting suspensions used at ∼6 wt % CNC separated into an upper isotropic phase and a lower anisotropic (chiral nematic) one in the course of quiescent standing.
25390070	8	22	theme	tensile	1395:1401	arg1	mode					1418:1421	tensile or compressive mode	1395:1421	tensile or compressive mode	1395:1421	In dynamic mechanical experiments in tensile or compressive mode, we observed a clear mechanical anisotropy for the polymer composites synthesized from wholly anisotropic suspensions under static or rotational magnetization.
25390070	1	23	theme	polymerization	374:387	arg1	treatment					389:397	polymerization treatment	374:397	polymerization treatment	374:397	Novel polymer composites reinforced with an oriented cellulose nanocrystal (CNC) assembly were prepared from suspensions of CNC in aqueous 2-hydroxyethyl methacrylate (HEMA) via magnetic field application to the suspensions followed by polymerization treatment.
25390070	1	24	theme	nanocrystal	201:211	arg1	assembly					219:226	an oriented cellulose nanocrystal (CNC) assembly	179:226	an oriented cellulose nanocrystal (CNC) assembly	179:226	Novel polymer composites reinforced with an oriented cellulose nanocrystal (CNC) assembly were prepared from suspensions of CNC in aqueous 2-hydroxyethyl methacrylate (HEMA) via magnetic field application to the suspensions followed by polymerization treatment.
25390070	8	25	theme	compressive	1406:1416	arg1	mode					1418:1421	tensile or compressive mode	1395:1421	tensile or compressive mode	1395:1421	In dynamic mechanical experiments in tensile or compressive mode, we observed a clear mechanical anisotropy for the polymer composites synthesized from wholly anisotropic suspensions under static or rotational magnetization.
25390070	0	26	theme	magnetic	121:128	arg1	fields					130:135	magnetic fields	121:135	magnetic fields	121:135	Anisotropic polymer composites synthesized by immobilizing cellulose nanocrystal suspensions specifically oriented under magnetic fields.
25390070	4	27	theme	oriented	811:818	arg1	composites					858:867	variously oriented CNC/poly(2-hydroxyethyl methacrylate) composites	801:867	variously oriented CNC/poly(2-hydroxyethyl methacrylate) composites	801:867	UV-induced polymerization of HEMA perpetuated the respective states of magnetic orientation invested for the CNC dispersions to yield variously oriented CNC/poly(2-hydroxyethyl methacrylate) composites.
25390070	2	28	theme	wt	436:437	arg1	CNC					441:443	∼6 wt % CNC	433:443	∼6 wt % CNC separated into an upper isotropic phase and a lower anisotropic (chiral nematic) one in the course of quiescent standing	433:564	The starting suspensions used at ∼6 wt % CNC separated into an upper isotropic phase and a lower anisotropic (chiral nematic) one in the course of quiescent standing.
25390070	6	29	theme	anisotropic	1139:1149	arg1	phases					1151:1156	the anisotropic phases	1135:1156	the anisotropic phases	1135:1156	The result indicated that CNCs were aligned in the composites distinctively according to the static or rotational magnetic application when the anisotropic phases were used, whereas such a specific CNC orientation was not appreciable when the isotropic phases were sampled.
25390070	0	30	theme	Anisotropic	0:10	arg1	composites					20:29	Anisotropic polymer composites	0:29	Anisotropic polymer composites	0:29	Anisotropic polymer composites synthesized by immobilizing cellulose nanocrystal suspensions specifically oriented under magnetic fields.
25390070	8	31	from	experiments	1380:1390	arg1	mode					1418:1421	tensile or compressive mode	1395:1421	tensile or compressive mode	1395:1421	In dynamic mechanical experiments in tensile or compressive mode, we observed a clear mechanical anisotropy for the polymer composites synthesized from wholly anisotropic suspensions under static or rotational magnetization.
25390070	2	32	theme	upper	463:467	arg1	phase					479:483	an upper isotropic phase	460:483	an upper isotropic phase	460:483	The starting suspensions used at ∼6 wt % CNC separated into an upper isotropic phase and a lower anisotropic (chiral nematic) one in the course of quiescent standing.
25390070	8	33	theme	clear	1438:1442	arg1	anisotropy					1455:1464	a clear mechanical anisotropy	1436:1464	a clear mechanical anisotropy for the polymer composites synthesized from wholly anisotropic suspensions under static or rotational magnetization	1436:1580	In dynamic mechanical experiments in tensile or compressive mode, we observed a clear mechanical anisotropy for the polymer composites synthesized from wholly anisotropic suspensions under static or rotational magnetization.
25390070	1	34	theme	magnetic	316:323	arg1	application					331:341	magnetic field application	316:341	magnetic field application to the suspensions followed by polymerization treatment	316:397	Novel polymer composites reinforced with an oriented cellulose nanocrystal (CNC) assembly were prepared from suspensions of CNC in aqueous 2-hydroxyethyl methacrylate (HEMA) via magnetic field application to the suspensions followed by polymerization treatment.
25390070	1	35	theme	Novel	138:142	arg1	composites					152:161	Novel polymer composites	138:161	Novel polymer composites reinforced with an oriented cellulose nanocrystal (CNC) assembly	138:226	Novel polymer composites reinforced with an oriented cellulose nanocrystal (CNC) assembly were prepared from suspensions of CNC in aqueous 2-hydroxyethyl methacrylate (HEMA) via magnetic field application to the suspensions followed by polymerization treatment.
25390070	6	36	theme	magnetic	1109:1116	arg1	application					1118:1128	the static or rotational magnetic application	1084:1128	the static or rotational magnetic application	1084:1128	The result indicated that CNCs were aligned in the composites distinctively according to the static or rotational magnetic application when the anisotropic phases were used, whereas such a specific CNC orientation was not appreciable when the isotropic phases were sampled.
25390070	2	37	theme	anisotropic	497:507	arg1	one					526:528	a lower anisotropic (chiral nematic) one	489:528	a lower anisotropic (chiral nematic) one in the course of quiescent standing	489:564	The starting suspensions used at ∼6 wt % CNC separated into an upper isotropic phase and a lower anisotropic (chiral nematic) one in the course of quiescent standing.
25390070	9	38	theme	uniaxial	1677:1684	arg1	alignment					1690:1698	the uniaxial CNC alignment	1673:1698	the uniaxial CNC alignment attainable through conversion of the initial chiral nematic phase into a nematic phase in the rotational magnetic field	1673:1818	The higher modulus (in compression) was detected for a composite reinforced by locking-in the uniaxial CNC alignment attainable through conversion of the initial chiral nematic phase into a nematic phase in the rotational magnetic field.
25390070	3	39	theme	anisotropic	647:657	arg1	phases					659:664	the isolated isotropic and anisotropic phases	620:664	the isolated isotropic and anisotropic phases	620:664	A static or rotational magnetic field was applied to the isolated isotropic and anisotropic phases.
25390070	0	40	theme	cellulose	59:67	arg1	suspensions					81:91	cellulose nanocrystal suspensions	59:91	cellulose nanocrystal suspensions specifically oriented under magnetic fields	59:135	Anisotropic polymer composites synthesized by immobilizing cellulose nanocrystal suspensions specifically oriented under magnetic fields.
25390070	6	41	theme	specific	1184:1191	arg1	orientation					1197:1207	such a specific CNC orientation	1177:1207	such a specific CNC orientation	1177:1207	The result indicated that CNCs were aligned in the composites distinctively according to the static or rotational magnetic application when the anisotropic phases were used, whereas such a specific CNC orientation was not appreciable when the isotropic phases were sampled.
25390070	6	41	theme	specific	1184:1191	arg1	appreciable					1217:1227	appreciable	1217:1227	appreciable	1217:1227	The result indicated that CNCs were aligned in the composites distinctively according to the static or rotational magnetic application when the anisotropic phases were used, whereas such a specific CNC orientation was not appreciable when the isotropic phases were sampled.
25390070	1	42	theme	oriented	182:189	arg1	assembly					219:226	an oriented cellulose nanocrystal (CNC) assembly	179:226	an oriented cellulose nanocrystal (CNC) assembly	179:226	Novel polymer composites reinforced with an oriented cellulose nanocrystal (CNC) assembly were prepared from suspensions of CNC in aqueous 2-hydroxyethyl methacrylate (HEMA) via magnetic field application to the suspensions followed by polymerization treatment.
25390070	4	43	theme	methacrylate	844:855	arg1	composites					858:867	variously oriented CNC/poly(2-hydroxyethyl methacrylate) composites	801:867	variously oriented CNC/poly(2-hydroxyethyl methacrylate) composites	801:867	UV-induced polymerization of HEMA perpetuated the respective states of magnetic orientation invested for the CNC dispersions to yield variously oriented CNC/poly(2-hydroxyethyl methacrylate) composites.
25390070	1	44	theme	2-hydroxyethyl	277:290	arg1	methacrylate					292:303	aqueous 2-hydroxyethyl methacrylate	269:303	aqueous 2-hydroxyethyl methacrylate (HEMA)	269:310	Novel polymer composites reinforced with an oriented cellulose nanocrystal (CNC) assembly were prepared from suspensions of CNC in aqueous 2-hydroxyethyl methacrylate (HEMA) via magnetic field application to the suspensions followed by polymerization treatment.
25390070	1	44	theme	2-hydroxyethyl	277:290	arg1	HEMA					306:309	HEMA	306:309	HEMA	306:309	Novel polymer composites reinforced with an oriented cellulose nanocrystal (CNC) assembly were prepared from suspensions of CNC in aqueous 2-hydroxyethyl methacrylate (HEMA) via magnetic field application to the suspensions followed by polymerization treatment.
25390070	9	45	theme	initial	1737:1743	arg1	phase					1760:1764	the initial chiral nematic phase	1733:1764	the initial chiral nematic phase	1733:1764	The higher modulus (in compression) was detected for a composite reinforced by locking-in the uniaxial CNC alignment attainable through conversion of the initial chiral nematic phase into a nematic phase in the rotational magnetic field.
25390070	7	46	theme	coherent	1303:1310	arg1	response					1312:1319	a coherent response	1301:1319	a coherent response of CNCs	1301:1327	This marks out effectiveness of a coherent response of CNCs in the mesomorphic assembly.
25390070	9	47	theme	nematic	1752:1758	arg1	phase					1760:1764	the initial chiral nematic phase	1733:1764	the initial chiral nematic phase	1733:1764	The higher modulus (in compression) was detected for a composite reinforced by locking-in the uniaxial CNC alignment attainable through conversion of the initial chiral nematic phase into a nematic phase in the rotational magnetic field.
25390070	6	48	theme	isotropic	1238:1246	arg1	phases					1248:1253	the isotropic phases	1234:1253	the isotropic phases	1234:1253	The result indicated that CNCs were aligned in the composites distinctively according to the static or rotational magnetic application when the anisotropic phases were used, whereas such a specific CNC orientation was not appreciable when the isotropic phases were sampled.
25390070	4	49	theme	UV-induced	667:676	arg1	polymerization					678:691	UV-induced polymerization	667:691	UV-induced polymerization of HEMA	667:699	UV-induced polymerization of HEMA perpetuated the respective states of magnetic orientation invested for the CNC dispersions to yield variously oriented CNC/poly(2-hydroxyethyl methacrylate) composites.
25390070	9	50	theme	nematic	1773:1779	arg1	phase					1781:1785	a nematic phase	1771:1785	a nematic phase in the rotational magnetic field	1771:1818	The higher modulus (in compression) was detected for a composite reinforced by locking-in the uniaxial CNC alignment attainable through conversion of the initial chiral nematic phase into a nematic phase in the rotational magnetic field.
25390070	5	51	theme	structural	874:883	arg1	characterization					885:900	The structural characterization	870:900	The structural characterization	870:900	The structural characterization was carried out by use of X-ray diffractometry and optical and scanning electron microscopy.
25390070	2	52	theme	standing	557:564	arg1	course					537:542	the course	533:542	the course of quiescent standing	533:564	The starting suspensions used at ∼6 wt % CNC separated into an upper isotropic phase and a lower anisotropic (chiral nematic) one in the course of quiescent standing.
25390070	8	53	theme	anisotropic	1517:1527	arg1	suspensions					1529:1539	wholly anisotropic suspensions	1510:1539	wholly anisotropic suspensions	1510:1539	In dynamic mechanical experiments in tensile or compressive mode, we observed a clear mechanical anisotropy for the polymer composites synthesized from wholly anisotropic suspensions under static or rotational magnetization.
25390070	5	54	theme	optical	953:959	arg1	microscopy					983:992	optical and scanning electron microscopy	953:992	microscopy	983:992	The structural characterization was carried out by use of X-ray diffractometry and optical and scanning electron microscopy.
25390070	7	55	theme	mesomorphic	1336:1346	arg1	assembly					1348:1355	the mesomorphic assembly	1332:1355	the mesomorphic assembly	1332:1355	This marks out effectiveness of a coherent response of CNCs in the mesomorphic assembly.
25390070	9	56	theme	rotational	1794:1803	arg1	field					1814:1818	the rotational magnetic field	1790:1818	the rotational magnetic field	1790:1818	The higher modulus (in compression) was detected for a composite reinforced by locking-in the uniaxial CNC alignment attainable through conversion of the initial chiral nematic phase into a nematic phase in the rotational magnetic field.
25390070	3	57	theme	magnetic	590:597	arg1	field					599:603	A static or rotational magnetic field	567:603	A static or rotational magnetic field	567:603	A static or rotational magnetic field was applied to the isolated isotropic and anisotropic phases.
25390070	6	58	used	used	1163:1166	arg2	phases					1151:1156	the anisotropic phases	1135:1156	the anisotropic phases	1135:1156	The result indicated that CNCs were aligned in the composites distinctively according to the static or rotational magnetic application when the anisotropic phases were used, whereas such a specific CNC orientation was not appreciable when the isotropic phases were sampled.
25390070	1	59	theme	polymer	144:150	arg1	composites					152:161	Novel polymer composites	138:161	Novel polymer composites reinforced with an oriented cellulose nanocrystal (CNC) assembly	138:226	Novel polymer composites reinforced with an oriented cellulose nanocrystal (CNC) assembly were prepared from suspensions of CNC in aqueous 2-hydroxyethyl methacrylate (HEMA) via magnetic field application to the suspensions followed by polymerization treatment.
25390070	3	60	theme	isolated	624:631	arg1	phases					659:664	the isolated isotropic and anisotropic phases	620:664	the isolated isotropic and anisotropic phases	620:664	A static or rotational magnetic field was applied to the isolated isotropic and anisotropic phases.
25390070	5	61	theme	electron	974:981	arg1	microscopy					983:992	optical and scanning electron microscopy	953:992	microscopy	983:992	The structural characterization was carried out by use of X-ray diffractometry and optical and scanning electron microscopy.
25390070	6	62	theme	static	1088:1093	arg1	application					1118:1128	the static or rotational magnetic application	1084:1128	the static or rotational magnetic application	1084:1128	The result indicated that CNCs were aligned in the composites distinctively according to the static or rotational magnetic application when the anisotropic phases were used, whereas such a specific CNC orientation was not appreciable when the isotropic phases were sampled.
25390070	2	63	used	used	425:428	arg2	suspensions					413:423	The starting suspensions	400:423	The starting suspensions	400:423	The starting suspensions used at ∼6 wt % CNC separated into an upper isotropic phase and a lower anisotropic (chiral nematic) one in the course of quiescent standing.
25390070	4	64	theme	orientation	747:757	arg1	states					728:733	the respective states	713:733	the respective states of magnetic orientation invested for the CNC dispersions	713:790	UV-induced polymerization of HEMA perpetuated the respective states of magnetic orientation invested for the CNC dispersions to yield variously oriented CNC/poly(2-hydroxyethyl methacrylate) composites.
25390070	5	65	theme	X-ray	928:932	arg1	diffractometry					934:947	X-ray diffractometry	928:947	X-ray diffractometry	928:947	The structural characterization was carried out by use of X-ray diffractometry and optical and scanning electron microscopy.
25390070	8	66	theme	mechanical	1369:1378	arg1	experiments					1380:1390	dynamic mechanical experiments	1361:1390	dynamic mechanical experiments in tensile or compressive mode	1361:1421	In dynamic mechanical experiments in tensile or compressive mode, we observed a clear mechanical anisotropy for the polymer composites synthesized from wholly anisotropic suspensions under static or rotational magnetization.
25390070	1	67	theme	cellulose	191:199	arg1	CNC					214:216	CNC	214:216	CNC	214:216	Novel polymer composites reinforced with an oriented cellulose nanocrystal (CNC) assembly were prepared from suspensions of CNC in aqueous 2-hydroxyethyl methacrylate (HEMA) via magnetic field application to the suspensions followed by polymerization treatment.
25390070	1	67	theme	cellulose	191:199	arg1	nanocrystal					201:211	cellulose nanocrystal	191:211	an oriented cellulose nanocrystal (CNC) assembly	179:226	Novel polymer composites reinforced with an oriented cellulose nanocrystal (CNC) assembly were prepared from suspensions of CNC in aqueous 2-hydroxyethyl methacrylate (HEMA) via magnetic field application to the suspensions followed by polymerization treatment.
25390070	2	68	from	phase	479:483	arg1	course					537:542	the course	533:542	the course of quiescent standing	533:564	The starting suspensions used at ∼6 wt % CNC separated into an upper isotropic phase and a lower anisotropic (chiral nematic) one in the course of quiescent standing.
25390070	4	69	theme	respective	717:726	arg1	states					728:733	the respective states	713:733	the respective states of magnetic orientation invested for the CNC dispersions	713:790	UV-induced polymerization of HEMA perpetuated the respective states of magnetic orientation invested for the CNC dispersions to yield variously oriented CNC/poly(2-hydroxyethyl methacrylate) composites.
25390070	8	70	theme	polymer	1474:1480	arg1	composites					1482:1491	the polymer composites	1470:1491	the polymer composites synthesized from wholly anisotropic suspensions under static or rotational magnetization	1470:1580	In dynamic mechanical experiments in tensile or compressive mode, we observed a clear mechanical anisotropy for the polymer composites synthesized from wholly anisotropic suspensions under static or rotational magnetization.
25390070	2	71	dep	anisotropic	497:507	arg1	nematic					517:523	chiral nematic	510:523	chiral nematic	510:523	The starting suspensions used at ∼6 wt % CNC separated into an upper isotropic phase and a lower anisotropic (chiral nematic) one in the course of quiescent standing.
25390070	9	72	from	phase	1781:1785	arg1	field					1814:1818	the rotational magnetic field	1790:1818	the rotational magnetic field	1790:1818	The higher modulus (in compression) was detected for a composite reinforced by locking-in the uniaxial CNC alignment attainable through conversion of the initial chiral nematic phase into a nematic phase in the rotational magnetic field.
25390070	2	73	theme	∼6	433:434	arg1	CNC					441:443	∼6 wt % CNC	433:443	∼6 wt % CNC separated into an upper isotropic phase and a lower anisotropic (chiral nematic) one in the course of quiescent standing	433:564	The starting suspensions used at ∼6 wt % CNC separated into an upper isotropic phase and a lower anisotropic (chiral nematic) one in the course of quiescent standing.
25390070	0	74	theme	polymer	12:18	arg1	composites					20:29	Anisotropic polymer composites	0:29	Anisotropic polymer composites	0:29	Anisotropic polymer composites synthesized by immobilizing cellulose nanocrystal suspensions specifically oriented under magnetic fields.
25390070	2	75	theme	isotropic	469:477	arg1	phase					479:483	an upper isotropic phase	460:483	an upper isotropic phase	460:483	The starting suspensions used at ∼6 wt % CNC separated into an upper isotropic phase and a lower anisotropic (chiral nematic) one in the course of quiescent standing.
25390070	4	76	theme	CNC	776:778	arg1	dispersions					780:790	the CNC dispersions	772:790	the CNC dispersions	772:790	UV-induced polymerization of HEMA perpetuated the respective states of magnetic orientation invested for the CNC dispersions to yield variously oriented CNC/poly(2-hydroxyethyl methacrylate) composites.
25390070	1	77	theme	field	325:329	arg1	application					331:341	magnetic field application	316:341	magnetic field application to the suspensions followed by polymerization treatment	316:397	Novel polymer composites reinforced with an oriented cellulose nanocrystal (CNC) assembly were prepared from suspensions of CNC in aqueous 2-hydroxyethyl methacrylate (HEMA) via magnetic field application to the suspensions followed by polymerization treatment.
25390070	6	78	theme	rotational	1098:1107	arg1	application					1118:1128	the static or rotational magnetic application	1084:1128	the static or rotational magnetic application	1084:1128	The result indicated that CNCs were aligned in the composites distinctively according to the static or rotational magnetic application when the anisotropic phases were used, whereas such a specific CNC orientation was not appreciable when the isotropic phases were sampled.
25390070	8	79	theme	mechanical	1444:1453	arg1	anisotropy					1455:1464	a clear mechanical anisotropy	1436:1464	a clear mechanical anisotropy for the polymer composites synthesized from wholly anisotropic suspensions under static or rotational magnetization	1436:1580	In dynamic mechanical experiments in tensile or compressive mode, we observed a clear mechanical anisotropy for the polymer composites synthesized from wholly anisotropic suspensions under static or rotational magnetization.
25390070	2	80	theme	%	439:439	arg1	CNC					441:443	∼6 wt % CNC	433:443	∼6 wt % CNC separated into an upper isotropic phase and a lower anisotropic (chiral nematic) one in the course of quiescent standing	433:564	The starting suspensions used at ∼6 wt % CNC separated into an upper isotropic phase and a lower anisotropic (chiral nematic) one in the course of quiescent standing.
25390070	0	81	theme	nanocrystal	69:79	arg1	suspensions					81:91	cellulose nanocrystal suspensions	59:91	cellulose nanocrystal suspensions specifically oriented under magnetic fields	59:135	Anisotropic polymer composites synthesized by immobilizing cellulose nanocrystal suspensions specifically oriented under magnetic fields.
25390070	9	82	theme	CNC	1686:1688	arg1	alignment					1690:1698	the uniaxial CNC alignment	1673:1698	the uniaxial CNC alignment attainable through conversion of the initial chiral nematic phase into a nematic phase in the rotational magnetic field	1673:1818	The higher modulus (in compression) was detected for a composite reinforced by locking-in the uniaxial CNC alignment attainable through conversion of the initial chiral nematic phase into a nematic phase in the rotational magnetic field.
25390070	6	83	theme	CNC	1193:1195	arg1	orientation					1197:1207	such a specific CNC orientation	1177:1207	such a specific CNC orientation	1177:1207	The result indicated that CNCs were aligned in the composites distinctively according to the static or rotational magnetic application when the anisotropic phases were used, whereas such a specific CNC orientation was not appreciable when the isotropic phases were sampled.
25390070	6	83	theme	CNC	1193:1195	arg1	appreciable					1217:1227	appreciable	1217:1227	appreciable	1217:1227	The result indicated that CNCs were aligned in the composites distinctively according to the static or rotational magnetic application when the anisotropic phases were used, whereas such a specific CNC orientation was not appreciable when the isotropic phases were sampled.
25390070	9	84	theme	attainable	1700:1709	arg1	alignment					1690:1698	the uniaxial CNC alignment	1673:1698	the uniaxial CNC alignment attainable through conversion of the initial chiral nematic phase into a nematic phase in the rotational magnetic field	1673:1818	The higher modulus (in compression) was detected for a composite reinforced by locking-in the uniaxial CNC alignment attainable through conversion of the initial chiral nematic phase into a nematic phase in the rotational magnetic field.
25390070	7	85	theme	CNCs	1324:1327	arg1	response					1312:1319	a coherent response	1301:1319	a coherent response of CNCs	1301:1327	This marks out effectiveness of a coherent response of CNCs in the mesomorphic assembly.
25390070	4	86	theme	CNC/poly	820:827	arg1	composites					858:867	variously oriented CNC/poly(2-hydroxyethyl methacrylate) composites	801:867	variously oriented CNC/poly(2-hydroxyethyl methacrylate) composites	801:867	UV-induced polymerization of HEMA perpetuated the respective states of magnetic orientation invested for the CNC dispersions to yield variously oriented CNC/poly(2-hydroxyethyl methacrylate) composites.
25390070	1	87	theme	CNC	262:264	arg1	suspensions					247:257	suspensions	247:257	suspensions of CNC in aqueous 2-hydroxyethyl methacrylate (HEMA)	247:310	Novel polymer composites reinforced with an oriented cellulose nanocrystal (CNC) assembly were prepared from suspensions of CNC in aqueous 2-hydroxyethyl methacrylate (HEMA) via magnetic field application to the suspensions followed by polymerization treatment.
25390070	7	88	theme	response	1312:1319	arg1	effectiveness					1284:1296	effectiveness	1284:1296	effectiveness of a coherent response of CNCs	1284:1327	This marks out effectiveness of a coherent response of CNCs in the mesomorphic assembly.
25390070	4	89	theme	2-hydroxyethyl	829:842	arg1	composites					858:867	variously oriented CNC/poly(2-hydroxyethyl methacrylate) composites	801:867	variously oriented CNC/poly(2-hydroxyethyl methacrylate) composites	801:867	UV-induced polymerization of HEMA perpetuated the respective states of magnetic orientation invested for the CNC dispersions to yield variously oriented CNC/poly(2-hydroxyethyl methacrylate) composites.
25390070	2	90	theme	starting	404:411	arg1	suspensions					413:423	The starting suspensions	400:423	The starting suspensions	400:423	The starting suspensions used at ∼6 wt % CNC separated into an upper isotropic phase and a lower anisotropic (chiral nematic) one in the course of quiescent standing.
25390070	1	91	theme	aqueous	269:275	arg1	methacrylate					292:303	aqueous 2-hydroxyethyl methacrylate	269:303	aqueous 2-hydroxyethyl methacrylate (HEMA)	269:310	Novel polymer composites reinforced with an oriented cellulose nanocrystal (CNC) assembly were prepared from suspensions of CNC in aqueous 2-hydroxyethyl methacrylate (HEMA) via magnetic field application to the suspensions followed by polymerization treatment.
25390070	1	91	theme	aqueous	269:275	arg1	HEMA					306:309	HEMA	306:309	HEMA	306:309	Novel polymer composites reinforced with an oriented cellulose nanocrystal (CNC) assembly were prepared from suspensions of CNC in aqueous 2-hydroxyethyl methacrylate (HEMA) via magnetic field application to the suspensions followed by polymerization treatment.
26088135	4	0	theme	authentic	471:479	arg1	trimers					487:493	authentic HIV-1 trimers	471:493	authentic HIV-1 trimers	471:493	However, preparation of authentic HIV-1 trimers has been challenging.
26088135	8	1	theme	long	982:985	arg1	linker					987:992	a long linker	980:992	a long linker separating the tag from the massive trimer base and glycan shield	980:1058	The C terminus of gp140 was attached to Strep-tag II with a long linker separating the tag from the massive trimer base and glycan shield.
26088135	8	2	theme	C	926:926	arg1	terminus					928:935	The C terminus	922:935	The C terminus of gp140	922:944	The C terminus of gp140 was attached to Strep-tag II with a long linker separating the tag from the massive trimer base and glycan shield.
26088135	1	3	theme	trimeric	138:145	arg1	spike					156:160	The trimeric envelope spike	134:160	The trimeric envelope spike of HIV-1	134:169	The trimeric envelope spike of HIV-1 mediates virus entry into human cells.
26088135	7	4	theme	new	863:865	arg1	approach					878:885	a new and simple approach	861:885	a new and simple approach to produce HIV-1 envelope trimers	861:919	Here, we report a new and simple approach to produce HIV-1 envelope trimers.
26088135	14	5	theme	HIV-1	1776:1780	arg1	system					1800:1805	a broadly applicable HIV-1 trimer production system	1755:1805	a broadly applicable HIV-1 trimer production system	1755:1805	These studies established a broadly applicable HIV-1 trimer production system as well as generating new insights into their assembly and maturation that collectively bear on the HIV-1 vaccine design.
26088135	5	6	used	used	605:608	arg2	column					539:544	an affinity column	527:544	an affinity column containing the broadly neutralizing antibody 2G12	527:594	Recently, an affinity column containing the broadly neutralizing antibody 2G12 has been used to capture recombinant gp140 and prepare trimers from clade A BG505 that naturally produces stable trimers.
26088135	6	7	theme	HIV-1	796:800	arg1	strains					802:808	the diverse HIV-1 strains	784:808	the diverse HIV-1 strains with different epitope signatures	784:842	However, this antibody-based approach may not be as effective for the diverse HIV-1 strains with different epitope signatures.
26088135	1	8	theme	envelope	147:154	arg1	spike					156:160	The trimeric envelope spike	134:160	The trimeric envelope spike of HIV-1	134:169	The trimeric envelope spike of HIV-1 mediates virus entry into human cells.
26088135	11	9	theme	propeller-shaped	1490:1505	arg1	trimers					1507:1513	compact three-blade propeller-shaped trimers	1470:1513	compact three-blade propeller-shaped trimers	1470:1513	Extensive biochemical characterizations showed that cleavage of gp140 was not essential for trimerization, but it triggered a conformational change that channels trimers into correct glycosylation pathways, generating compact three-blade propeller-shaped trimers.
26088135	10	10	theme	uncleaved	1153:1161	arg1	trimers					1200:1206	Cleaved, uncleaved, and fully or partially glycosylated trimers	1144:1206	Cleaved, uncleaved, and fully or partially glycosylated trimers from different clade viruses	1144:1235	Cleaved, uncleaved, and fully or partially glycosylated trimers from different clade viruses were produced.
26088135	3	11	theme	virus	430:434	arg1	infection					436:444	virus infection	430:444	virus infection	430:444	A recombinant vaccine that mimics the native trimer might elicit entry-blocking antibodies and prevent virus infection.
26088135	4	12	theme	trimers	487:493	arg1	preparation					456:466	preparation	456:466	preparation of authentic HIV-1 trimers	456:493	However, preparation of authentic HIV-1 trimers has been challenging.
26088135	10	13	gly	glycosylated	1187:1198	arg1	trimers					1200:1206	Cleaved, uncleaved, and fully or partially glycosylated trimers	1144:1206	Cleaved, uncleaved, and fully or partially glycosylated trimers from different clade viruses	1144:1235	Cleaved, uncleaved, and fully or partially glycosylated trimers from different clade viruses were produced.
26088135	6	14	theme	diverse	788:794	arg1	strains					802:808	the diverse HIV-1 strains	784:808	the diverse HIV-1 strains with different epitope signatures	784:842	However, this antibody-based approach may not be as effective for the diverse HIV-1 strains with different epitope signatures.
26088135	10	15	theme	glycosylated	1187:1198	arg1	trimers					1200:1206	Cleaved, uncleaved, and fully or partially glycosylated trimers	1144:1206	Cleaved, uncleaved, and fully or partially glycosylated trimers from different clade viruses	1144:1235	Cleaved, uncleaved, and fully or partially glycosylated trimers from different clade viruses were produced.
26088135	14	16	theme	trimer	1782:1787	arg1	system					1800:1805	a broadly applicable HIV-1 trimer production system	1755:1805	a broadly applicable HIV-1 trimer production system	1755:1805	These studies established a broadly applicable HIV-1 trimer production system as well as generating new insights into their assembly and maturation that collectively bear on the HIV-1 vaccine design.
26088135	0	17	theme	PROPER	68:73	arg1	GLYCOSYLATION					75:87	PROPER GLYCOSYLATION	68:87	PROPER GLYCOSYLATION	68:87	A New Approach to Produce HIV-1 Envelope Trimers: BOTH CLEAVAGE AND PROPER GLYCOSYLATION ARE ESSENTIAL TO GENERATE AUTHENTIC TRIMERS.
26088135	8	18	theme	massive	1022:1028	arg1	base					1037:1040	the massive trimer base	1018:1040	the massive trimer base	1018:1040	The C terminus of gp140 was attached to Strep-tag II with a long linker separating the tag from the massive trimer base and glycan shield.
26088135	10	19	theme	Cleaved	1144:1150	arg1	trimers					1200:1206	Cleaved, uncleaved, and fully or partially glycosylated trimers	1144:1206	Cleaved, uncleaved, and fully or partially glycosylated trimers from different clade viruses	1144:1235	Cleaved, uncleaved, and fully or partially glycosylated trimers from different clade viruses were produced.
26088135	11	20	theme	conformational	1378:1391	arg1	change					1393:1398	a conformational change	1376:1398	a conformational change that channels trimers into correct glycosylation pathways, generating compact three-blade propeller-shaped trimers	1376:1513	Extensive biochemical characterizations showed that cleavage of gp140 was not essential for trimerization, but it triggered a conformational change that channels trimers into correct glycosylation pathways, generating compact three-blade propeller-shaped trimers.
26088135	11	21	theme	biochemical	1262:1272	arg1	characterizations					1274:1290	Extensive biochemical characterizations	1252:1290	Extensive biochemical characterizations	1252:1290	Extensive biochemical characterizations showed that cleavage of gp140 was not essential for trimerization, but it triggered a conformational change that channels trimers into correct glycosylation pathways, generating compact three-blade propeller-shaped trimers.
26088135	0	22	theme	Envelope	32:39	arg1	Trimers					41:47	HIV-1 Envelope Trimers	26:47	HIV-1 Envelope Trimers	26:47	A New Approach to Produce HIV-1 Envelope Trimers: BOTH CLEAVAGE AND PROPER GLYCOSYLATION ARE ESSENTIAL TO GENERATE AUTHENTIC TRIMERS.
26088135	10	23	from	viruses	1229:1235	arg1	trimers					1200:1206	Cleaved, uncleaved, and fully or partially glycosylated trimers	1144:1206	Cleaved, uncleaved, and fully or partially glycosylated trimers from different clade viruses	1144:1235	Cleaved, uncleaved, and fully or partially glycosylated trimers from different clade viruses were produced.
26088135	7	24	theme	envelope	904:911	arg1	trimers					913:919	HIV-1 envelope trimers	898:919	HIV-1 envelope trimers	898:919	Here, we report a new and simple approach to produce HIV-1 envelope trimers.
26088135	14	25	theme	new	1829:1831	arg1	insights					1833:1840	new insights	1829:1840	new insights into their assembly and maturation that collectively bear on the HIV-1 vaccine design	1829:1926	These studies established a broadly applicable HIV-1 trimer production system as well as generating new insights into their assembly and maturation that collectively bear on the HIV-1 vaccine design.
26088135	0	26	theme	New	2:4	arg1	Approach					6:13	A New Approach	0:13	A New Approach	0:13	A New Approach to Produce HIV-1 Envelope Trimers: BOTH CLEAVAGE AND PROPER GLYCOSYLATION ARE ESSENTIAL TO GENERATE AUTHENTIC TRIMERS.
26088135	11	27	theme	Extensive	1252:1260	arg1	characterizations					1274:1290	Extensive biochemical characterizations	1252:1290	Extensive biochemical characterizations	1252:1290	Extensive biochemical characterizations showed that cleavage of gp140 was not essential for trimerization, but it triggered a conformational change that channels trimers into correct glycosylation pathways, generating compact three-blade propeller-shaped trimers.
26088135	2	28	theme	associated	279:288	arg1	gp120					300:304	gp120	300:304	gp120	300:304	The exposed part of the trimer, gp140, consists of two noncovalently associated subunits, gp120 and gp41 ectodomain.
26088135	2	28	theme	associated	279:288	arg1	ectodomain					315:324	gp41 ectodomain	310:324	gp41 ectodomain	310:324	The exposed part of the trimer, gp140, consists of two noncovalently associated subunits, gp120 and gp41 ectodomain.
26088135	2	28	theme	associated	279:288	arg1	subunits					290:297	two noncovalently associated subunits	261:297	two noncovalently associated subunits	261:297	The exposed part of the trimer, gp140, consists of two noncovalently associated subunits, gp120 and gp41 ectodomain.
26088135	10	29	theme	clade	1223:1227	arg1	viruses					1229:1235	different clade viruses	1213:1235	different clade viruses	1213:1235	Cleaved, uncleaved, and fully or partially glycosylated trimers from different clade viruses were produced.
26088135	1	30	theme	HIV-1	165:169	arg1	spike					156:160	The trimeric envelope spike	134:160	The trimeric envelope spike of HIV-1	134:169	The trimeric envelope spike of HIV-1 mediates virus entry into human cells.
26088135	12	31	theme	conformational	1625:1638	arg1	heterogeneity					1640:1652	conformational heterogeneity	1625:1652	conformational heterogeneity	1625:1652	Uncleaved trimers entered aberrant pathways, resulting in hyperglycosylation, nonspecific cross-linking, and conformational heterogeneity.
26088135	5	32	theme	recombinant	621:631	arg1	gp140					633:637	recombinant gp140	621:637	recombinant gp140	621:637	Recently, an affinity column containing the broadly neutralizing antibody 2G12 has been used to capture recombinant gp140 and prepare trimers from clade A BG505 that naturally produces stable trimers.
26088135	5	33	theme	broadly	561:567	arg1	antibody					582:589	the broadly neutralizing antibody 2G12	557:594	the broadly neutralizing antibody 2G12	557:594	Recently, an affinity column containing the broadly neutralizing antibody 2G12 has been used to capture recombinant gp140 and prepare trimers from clade A BG505 that naturally produces stable trimers.
26088135	11	34	theme	glycosylation	1435:1447	arg1	pathways					1449:1456	correct glycosylation pathways	1427:1456	correct glycosylation pathways	1427:1456	Extensive biochemical characterizations showed that cleavage of gp140 was not essential for trimerization, but it triggered a conformational change that channels trimers into correct glycosylation pathways, generating compact three-blade propeller-shaped trimers.
26088135	3	35	theme	entry-blocking	392:405	arg1	antibodies					407:416	entry-blocking antibodies	392:416	entry-blocking antibodies	392:416	A recombinant vaccine that mimics the native trimer might elicit entry-blocking antibodies and prevent virus infection.
26088135	3	36	theme	recombinant	329:339	arg1	vaccine					341:347	A recombinant vaccine	327:347	A recombinant vaccine that mimics the native trimer	327:377	A recombinant vaccine that mimics the native trimer might elicit entry-blocking antibodies and prevent virus infection.
26088135	8	37	theme	trimer	1030:1035	arg1	base					1037:1040	the massive trimer base	1018:1040	the massive trimer base	1018:1040	The C terminus of gp140 was attached to Strep-tag II with a long linker separating the tag from the massive trimer base and glycan shield.
26088135	5	38	theme	neutralizing	569:580	arg1	antibody					582:589	the broadly neutralizing antibody 2G12	557:594	the broadly neutralizing antibody 2G12	557:594	Recently, an affinity column containing the broadly neutralizing antibody 2G12 has been used to capture recombinant gp140 and prepare trimers from clade A BG505 that naturally produces stable trimers.
26088135	14	39	theme	production	1789:1798	arg1	system					1800:1805	a broadly applicable HIV-1 trimer production system	1755:1805	a broadly applicable HIV-1 trimer production system	1755:1805	These studies established a broadly applicable HIV-1 trimer production system as well as generating new insights into their assembly and maturation that collectively bear on the HIV-1 vaccine design.
26088135	8	40	theme	gp140	940:944	arg1	terminus					928:935	The C terminus	922:935	The C terminus of gp140	922:944	The C terminus of gp140 was attached to Strep-tag II with a long linker separating the tag from the massive trimer base and glycan shield.
26088135	1	41	theme	virus	180:184	arg1	entry					186:190	virus entry	180:190	virus entry into human cells	180:207	The trimeric envelope spike of HIV-1 mediates virus entry into human cells.
26088135	6	42	theme	antibody-based	732:745	arg1	approach					747:754	this antibody-based approach	727:754	this antibody-based approach	727:754	However, this antibody-based approach may not be as effective for the diverse HIV-1 strains with different epitope signatures.
26088135	14	43	theme	applicable	1765:1774	arg1	system					1800:1805	a broadly applicable HIV-1 trimer production system	1755:1805	a broadly applicable HIV-1 trimer production system	1755:1805	These studies established a broadly applicable HIV-1 trimer production system as well as generating new insights into their assembly and maturation that collectively bear on the HIV-1 vaccine design.
26088135	5	44	theme	stable	702:707	arg1	trimers					709:715	stable trimers	702:715	stable trimers	702:715	Recently, an affinity column containing the broadly neutralizing antibody 2G12 has been used to capture recombinant gp140 and prepare trimers from clade A BG505 that naturally produces stable trimers.
26088135	6	45	with	strains	802:808	arg1	signatures					833:842	different epitope signatures	815:842	different epitope signatures	815:842	However, this antibody-based approach may not be as effective for the diverse HIV-1 strains with different epitope signatures.
26088135	0	46	theme	HIV-1	26:30	arg1	Trimers					41:47	HIV-1 Envelope Trimers	26:47	HIV-1 Envelope Trimers	26:47	A New Approach to Produce HIV-1 Envelope Trimers: BOTH CLEAVAGE AND PROPER GLYCOSYLATION ARE ESSENTIAL TO GENERATE AUTHENTIC TRIMERS.
26088135	2	47	theme	exposed	214:220	arg1	gp140					242:246	gp140	242:246	gp140	242:246	The exposed part of the trimer, gp140, consists of two noncovalently associated subunits, gp120 and gp41 ectodomain.
26088135	2	47	theme	exposed	214:220	arg1	part					222:225	The exposed part	210:225	The exposed part of the trimer	210:239	The exposed part of the trimer, gp140, consists of two noncovalently associated subunits, gp120 and gp41 ectodomain.
26088135	11	48	theme	correct	1427:1433	arg1	pathways					1449:1456	correct glycosylation pathways	1427:1456	correct glycosylation pathways	1427:1456	Extensive biochemical characterizations showed that cleavage of gp140 was not essential for trimerization, but it triggered a conformational change that channels trimers into correct glycosylation pathways, generating compact three-blade propeller-shaped trimers.
26088135	12	49	theme	nonspecific	1594:1604	arg1	cross-linking					1606:1618	nonspecific cross-linking	1594:1618	nonspecific cross-linking	1594:1618	Uncleaved trimers entered aberrant pathways, resulting in hyperglycosylation, nonspecific cross-linking, and conformational heterogeneity.
26088135	0	50	theme	AUTHENTIC	115:123	arg1	TRIMERS					125:131	AUTHENTIC TRIMERS	115:131	AUTHENTIC TRIMERS	115:131	A New Approach to Produce HIV-1 Envelope Trimers: BOTH CLEAVAGE AND PROPER GLYCOSYLATION ARE ESSENTIAL TO GENERATE AUTHENTIC TRIMERS.
26088135	12	51	theme	Uncleaved	1516:1524	arg1	trimers					1526:1532	Uncleaved trimers	1516:1532	Uncleaved trimers	1516:1532	Uncleaved trimers entered aberrant pathways, resulting in hyperglycosylation, nonspecific cross-linking, and conformational heterogeneity.
26088135	5	52	contain	containing	546:555	arg2	antibody					582:589	the broadly neutralizing antibody 2G12	557:594	the broadly neutralizing antibody 2G12	557:594	Recently, an affinity column containing the broadly neutralizing antibody 2G12 has been used to capture recombinant gp140 and prepare trimers from clade A BG505 that naturally produces stable trimers.
26088135	5	52	contain	containing	546:555	arg1	column					539:544	an affinity column	527:544	an affinity column containing the broadly neutralizing antibody 2G12	527:594	Recently, an affinity column containing the broadly neutralizing antibody 2G12 has been used to capture recombinant gp140 and prepare trimers from clade A BG505 that naturally produces stable trimers.
26088135	11	53	theme	gp140	1316:1320	arg1	cleavage					1304:1311	cleavage	1304:1311	cleavage of gp140	1304:1320	Extensive biochemical characterizations showed that cleavage of gp140 was not essential for trimerization, but it triggered a conformational change that channels trimers into correct glycosylation pathways, generating compact three-blade propeller-shaped trimers.
26088135	3	54	theme	native	365:370	arg1	trimer					372:377	the native trimer	361:377	the native trimer	361:377	A recombinant vaccine that mimics the native trimer might elicit entry-blocking antibodies and prevent virus infection.
26088135	13	55	theme	gp41	1709:1712	arg1	glycosylation					1714:1726	gp41 glycosylation	1709:1726	gp41 glycosylation	1709:1726	Even the cleaved trimers showed microheterogeneity in gp41 glycosylation.
26088135	5	56	theme	clade	664:668	arg1	A					670:670	clade A BG505	664:676	clade A BG505	664:676	Recently, an affinity column containing the broadly neutralizing antibody 2G12 has been used to capture recombinant gp140 and prepare trimers from clade A BG505 that naturally produces stable trimers.
26088135	6	57	theme	epitope	825:831	arg1	signatures					833:842	different epitope signatures	815:842	different epitope signatures	815:842	However, this antibody-based approach may not be as effective for the diverse HIV-1 strains with different epitope signatures.
26088135	5	58	theme	affinity	530:537	arg1	column					539:544	an affinity column	527:544	an affinity column containing the broadly neutralizing antibody 2G12	527:594	Recently, an affinity column containing the broadly neutralizing antibody 2G12 has been used to capture recombinant gp140 and prepare trimers from clade A BG505 that naturally produces stable trimers.
26088135	11	59	theme	compact	1470:1476	arg1	trimers					1507:1513	compact three-blade propeller-shaped trimers	1470:1513	compact three-blade propeller-shaped trimers	1470:1513	Extensive biochemical characterizations showed that cleavage of gp140 was not essential for trimerization, but it triggered a conformational change that channels trimers into correct glycosylation pathways, generating compact three-blade propeller-shaped trimers.
26088135	7	60	theme	HIV-1	898:902	arg1	trimers					913:919	HIV-1 envelope trimers	898:919	HIV-1 envelope trimers	898:919	Here, we report a new and simple approach to produce HIV-1 envelope trimers.
26088135	13	61	theme	cleaved	1664:1670	arg1	trimers					1672:1678	Even the cleaved trimers	1655:1678	Even the cleaved trimers	1655:1678	Even the cleaved trimers showed microheterogeneity in gp41 glycosylation.
26088135	6	62	theme	different	815:823	arg1	signatures					833:842	different epitope signatures	815:842	different epitope signatures	815:842	However, this antibody-based approach may not be as effective for the diverse HIV-1 strains with different epitope signatures.
26088135	2	63	theme	trimer	234:239	arg1	gp140					242:246	gp140	242:246	gp140	242:246	The exposed part of the trimer, gp140, consists of two noncovalently associated subunits, gp120 and gp41 ectodomain.
26088135	2	63	theme	trimer	234:239	arg1	part					222:225	The exposed part	210:225	The exposed part of the trimer	210:239	The exposed part of the trimer, gp140, consists of two noncovalently associated subunits, gp120 and gp41 ectodomain.
26088135	9	64	theme	culture	1128:1134	arg1	medium					1136:1141	the culture medium	1124:1141	the culture medium	1124:1141	This allowed capture of nearly homogeneous gp140 directly from the culture medium.
26088135	14	65	theme	vaccine	1913:1919	arg1	design					1921:1926	the HIV-1 vaccine design	1903:1926	the HIV-1 vaccine design	1903:1926	These studies established a broadly applicable HIV-1 trimer production system as well as generating new insights into their assembly and maturation that collectively bear on the HIV-1 vaccine design.
26088135	11	66	theme	three-blade	1478:1488	arg1	trimers					1507:1513	compact three-blade propeller-shaped trimers	1470:1513	compact three-blade propeller-shaped trimers	1470:1513	Extensive biochemical characterizations showed that cleavage of gp140 was not essential for trimerization, but it triggered a conformational change that channels trimers into correct glycosylation pathways, generating compact three-blade propeller-shaped trimers.
26088135	5	67	from	A	670:670	arg1	trimers					651:657	trimers	651:657	trimers from clade A BG505 that naturally produces stable trimers	651:715	Recently, an affinity column containing the broadly neutralizing antibody 2G12 has been used to capture recombinant gp140 and prepare trimers from clade A BG505 that naturally produces stable trimers.
26088135	9	68	theme	homogeneous	1092:1102	arg1	gp140					1104:1108	nearly homogeneous gp140	1085:1108	nearly homogeneous gp140	1085:1108	This allowed capture of nearly homogeneous gp140 directly from the culture medium.
26088135	14	69	theme	HIV-1	1907:1911	arg1	design					1921:1926	the HIV-1 vaccine design	1903:1926	the HIV-1 vaccine design	1903:1926	These studies established a broadly applicable HIV-1 trimer production system as well as generating new insights into their assembly and maturation that collectively bear on the HIV-1 vaccine design.
26088135	13	70	from	microheterogeneity	1687:1704	arg1	glycosylation					1714:1726	gp41 glycosylation	1709:1726	gp41 glycosylation	1709:1726	Even the cleaved trimers showed microheterogeneity in gp41 glycosylation.
26088135	1	71	theme	human	197:201	arg1	cells					203:207	human cells	197:207	human cells	197:207	The trimeric envelope spike of HIV-1 mediates virus entry into human cells.
26088135	12	72	theme	aberrant	1542:1549	arg1	pathways					1551:1558	aberrant pathways	1542:1558	aberrant pathways	1542:1558	Uncleaved trimers entered aberrant pathways, resulting in hyperglycosylation, nonspecific cross-linking, and conformational heterogeneity.
26088135	9	73	theme	gp140	1104:1108	arg1	capture					1074:1080	capture	1074:1080	capture of nearly homogeneous gp140 directly from the culture medium	1074:1141	This allowed capture of nearly homogeneous gp140 directly from the culture medium.
26088135	9	74	from	medium	1136:1141	arg1	capture					1074:1080	capture	1074:1080	capture of nearly homogeneous gp140 directly from the culture medium	1074:1141	This allowed capture of nearly homogeneous gp140 directly from the culture medium.
26088135	7	75	theme	simple	871:876	arg1	approach					878:885	a new and simple approach	861:885	a new and simple approach to produce HIV-1 envelope trimers	861:919	Here, we report a new and simple approach to produce HIV-1 envelope trimers.
26088135	4	76	theme	HIV-1	481:485	arg1	trimers					487:493	authentic HIV-1 trimers	471:493	authentic HIV-1 trimers	471:493	However, preparation of authentic HIV-1 trimers has been challenging.
26088135	10	77	theme	different	1213:1221	arg1	viruses					1229:1235	different clade viruses	1213:1235	different clade viruses	1213:1235	Cleaved, uncleaved, and fully or partially glycosylated trimers from different clade viruses were produced.
26088135	8	78	attach	attached	950:957	arg2	terminus					928:935	The C terminus	922:935	The C terminus of gp140	922:944	The C terminus of gp140 was attached to Strep-tag II with a long linker separating the tag from the massive trimer base and glycan shield.
26088135	8	78	attach	attached	950:957	arg1	Strep-tag					962:970	Strep-tag II	962:973	Strep-tag II	962:973	The C terminus of gp140 was attached to Strep-tag II with a long linker separating the tag from the massive trimer base and glycan shield.
27226567	0	0	theme	Disaccharide	96:107	arg1	Composition					109:119	a Defined Disaccharide Composition	86:119	a Defined Disaccharide Composition	86:119	Xyloside-primed Chondroitin Sulfate/Dermatan Sulfate from Breast Carcinoma Cells with a Defined Disaccharide Composition Has Cytotoxic Effects in Vitro.
27226567	1	1	theme	cell	533:536	arg1	type					538:541	cell type	533:541	cell type	533:541	We previously reported that the xyloside 2-(6-hydroxynaphthyl) β-d-xylopyranoside (XylNapOH), in contrast to 2-naphthyl β-d-xylopyranoside (XylNap), specifically reduces tumor growth both in vitro and in vivo Although there are indications that this could be mediated by the xyloside-primed glycosaminoglycans (GAGs) and that these differ in composition depending on xyloside and cell type, detailed knowledge regarding a structure-function relationship is lacking.
27226567	2	2	theme	sulfate/dermatan	834:849	arg1	GAGs					859:862	both XylNapOH- and XylNap-primed chondroitin sulfate/dermatan sulfate GAGs	789:862	both XylNapOH- and XylNap-primed chondroitin sulfate/dermatan sulfate GAGs derived from HCC70 cells	789:887	In this study we isolated XylNapOH- and XylNap-primed GAGs from a breast carcinoma cell line, HCC70, and a breast fibroblast cell line, CCD-1095Sk, and demonstrated that both XylNapOH- and XylNap-primed chondroitin sulfate/dermatan sulfate GAGs derived from HCC70 cells had a cytotoxic effect on HCC70 cells and CCD-1095Sk cells.
27226567	2	3	theme	cytotoxic	895:903	arg1	effect					905:910	a cytotoxic effect	893:910	a cytotoxic effect	893:910	In this study we isolated XylNapOH- and XylNap-primed GAGs from a breast carcinoma cell line, HCC70, and a breast fibroblast cell line, CCD-1095Sk, and demonstrated that both XylNapOH- and XylNap-primed chondroitin sulfate/dermatan sulfate GAGs derived from HCC70 cells had a cytotoxic effect on HCC70 cells and CCD-1095Sk cells.
27226567	0	4	theme	Defined	88:94	arg1	Composition					109:119	a Defined Disaccharide Composition	86:119	a Defined Disaccharide Composition	86:119	Xyloside-primed Chondroitin Sulfate/Dermatan Sulfate from Breast Carcinoma Cells with a Defined Disaccharide Composition Has Cytotoxic Effects in Vitro.
27226567	1	5	theme	xyloside-primed	428:442	arg1	GAGs					464:467	GAGs	464:467	GAGs	464:467	We previously reported that the xyloside 2-(6-hydroxynaphthyl) β-d-xylopyranoside (XylNapOH), in contrast to 2-naphthyl β-d-xylopyranoside (XylNap), specifically reduces tumor growth both in vitro and in vivo Although there are indications that this could be mediated by the xyloside-primed glycosaminoglycans (GAGs) and that these differ in composition depending on xyloside and cell type, detailed knowledge regarding a structure-function relationship is lacking.
27226567	1	5	theme	xyloside-primed	428:442	arg1	glycosaminoglycans					444:461	the xyloside-primed glycosaminoglycans	424:461	the xyloside-primed glycosaminoglycans (GAGs)	424:468	We previously reported that the xyloside 2-(6-hydroxynaphthyl) β-d-xylopyranoside (XylNapOH), in contrast to 2-naphthyl β-d-xylopyranoside (XylNap), specifically reduces tumor growth both in vitro and in vivo Although there are indications that this could be mediated by the xyloside-primed glycosaminoglycans (GAGs) and that these differ in composition depending on xyloside and cell type, detailed knowledge regarding a structure-function relationship is lacking.
27226567	4	6	theme	cells	1300:1304	arg1	growth					1284:1289	the growth	1280:1289	the growth of HCC70 cells or CCD-105Sk cells	1280:1323	In contrast, neither the chondroitin sulfate/dermatan sulfate nor the heparan sulfate derived from CCD-1095Sk cells primed on XylNapOH or XylNap had any effect on the growth of HCC70 cells or CCD-105Sk cells.
27226567	2	7	theme	chondroitin	822:832	arg1	GAGs					859:862	both XylNapOH- and XylNap-primed chondroitin sulfate/dermatan sulfate GAGs	789:862	both XylNapOH- and XylNap-primed chondroitin sulfate/dermatan sulfate GAGs derived from HCC70 cells	789:887	In this study we isolated XylNapOH- and XylNap-primed GAGs from a breast carcinoma cell line, HCC70, and a breast fibroblast cell line, CCD-1095Sk, and demonstrated that both XylNapOH- and XylNap-primed chondroitin sulfate/dermatan sulfate GAGs derived from HCC70 cells had a cytotoxic effect on HCC70 cells and CCD-1095Sk cells.
27226567	1	8	theme	2-naphthyl	262:271	arg1	XylNap					293:298	XylNap	293:298	XylNap	293:298	We previously reported that the xyloside 2-(6-hydroxynaphthyl) β-d-xylopyranoside (XylNapOH), in contrast to 2-naphthyl β-d-xylopyranoside (XylNap), specifically reduces tumor growth both in vitro and in vivo Although there are indications that this could be mediated by the xyloside-primed glycosaminoglycans (GAGs) and that these differ in composition depending on xyloside and cell type, detailed knowledge regarding a structure-function relationship is lacking.
27226567	1	8	theme	2-naphthyl	262:271	arg1	β-d-xylopyranoside					273:290	2-naphthyl β-d-xylopyranoside	262:290	2-naphthyl β-d-xylopyranoside (XylNap)	262:299	We previously reported that the xyloside 2-(6-hydroxynaphthyl) β-d-xylopyranoside (XylNapOH), in contrast to 2-naphthyl β-d-xylopyranoside (XylNap), specifically reduces tumor growth both in vitro and in vivo Although there are indications that this could be mediated by the xyloside-primed glycosaminoglycans (GAGs) and that these differ in composition depending on xyloside and cell type, detailed knowledge regarding a structure-function relationship is lacking.
27226567	3	9	theme	concentration-dependent	1043:1065	arg1	manner					1067:1072	a concentration-dependent manner	1041:1072	a concentration-dependent manner	1041:1072	The cytotoxic effect appeared to be mediated by induction of apoptosis and was inhibited in a concentration-dependent manner by the XylNap-primed heparan sulfate GAGs.
27226567	2	10	theme	XylNap-primed	659:671	arg1	GAGs					673:676	XylNap-primed GAGs	659:676	XylNap-primed GAGs	659:676	In this study we isolated XylNapOH- and XylNap-primed GAGs from a breast carcinoma cell line, HCC70, and a breast fibroblast cell line, CCD-1095Sk, and demonstrated that both XylNapOH- and XylNap-primed chondroitin sulfate/dermatan sulfate GAGs derived from HCC70 cells had a cytotoxic effect on HCC70 cells and CCD-1095Sk cells.
27226567	2	11	theme	XylNap-primed	808:820	arg1	GAGs					859:862	both XylNapOH- and XylNap-primed chondroitin sulfate/dermatan sulfate GAGs	789:862	both XylNapOH- and XylNap-primed chondroitin sulfate/dermatan sulfate GAGs derived from HCC70 cells	789:887	In this study we isolated XylNapOH- and XylNap-primed GAGs from a breast carcinoma cell line, HCC70, and a breast fibroblast cell line, CCD-1095Sk, and demonstrated that both XylNapOH- and XylNap-primed chondroitin sulfate/dermatan sulfate GAGs derived from HCC70 cells had a cytotoxic effect on HCC70 cells and CCD-1095Sk cells.
27226567	0	12	from	Cells	75:79	arg1	Sulfate					45:51	Xyloside-primed Chondroitin Sulfate/Dermatan Sulfate	0:51	Xyloside-primed Chondroitin Sulfate/Dermatan Sulfate from Breast Carcinoma Cells with a Defined Disaccharide Composition	0:119	Xyloside-primed Chondroitin Sulfate/Dermatan Sulfate from Breast Carcinoma Cells with a Defined Disaccharide Composition Has Cytotoxic Effects in Vitro.
27226567	6	13	theme	sulfate/dermatan	1628:1643	arg1	sulfate					1645:1651	chondroitin sulfate/dermatan sulfate	1616:1651	chondroitin sulfate/dermatan sulfate	1616:1651	To our knowledge this is the first report on cytotoxic effects mediated by chondroitin sulfate/dermatan sulfate.
27226567	4	14	theme	chondroitin	1142:1152	arg1	sulfate					1171:1177	the chondroitin sulfate/dermatan sulfate	1138:1177	the chondroitin sulfate/dermatan sulfate	1138:1177	In contrast, neither the chondroitin sulfate/dermatan sulfate nor the heparan sulfate derived from CCD-1095Sk cells primed on XylNapOH or XylNap had any effect on the growth of HCC70 cells or CCD-105Sk cells.
27226567	4	15	theme	CCD-105Sk	1309:1317	arg1	cells					1319:1323	CCD-105Sk cells	1309:1323	CCD-105Sk cells	1309:1323	In contrast, neither the chondroitin sulfate/dermatan sulfate nor the heparan sulfate derived from CCD-1095Sk cells primed on XylNapOH or XylNap had any effect on the growth of HCC70 cells or CCD-105Sk cells.
27226567	3	16	theme	sulfate	1103:1109	arg1	GAGs					1111:1114	the XylNap-primed heparan sulfate GAGs	1077:1114	the XylNap-primed heparan sulfate GAGs	1077:1114	The cytotoxic effect appeared to be mediated by induction of apoptosis and was inhibited in a concentration-dependent manner by the XylNap-primed heparan sulfate GAGs.
27226567	5	17	theme	cell	1462:1465	arg1	lines					1467:1471	the two cell lines	1454:1471	the two cell lines	1454:1471	These observations were related to the disaccharide composition of the XylNapOH- and XylNap-primed GAGs, which differed between the two cell lines but was similar when the GAGs were derived from the same cell line.
27226567	1	18	theme	xyloside	185:192	arg1	β-d-xylopyranoside					216:233	the xyloside 2-(6-hydroxynaphthyl) β-d-xylopyranoside	181:233	the xyloside 2-(6-hydroxynaphthyl) β-d-xylopyranoside (XylNapOH)	181:244	We previously reported that the xyloside 2-(6-hydroxynaphthyl) β-d-xylopyranoside (XylNapOH), in contrast to 2-naphthyl β-d-xylopyranoside (XylNap), specifically reduces tumor growth both in vitro and in vivo Although there are indications that this could be mediated by the xyloside-primed glycosaminoglycans (GAGs) and that these differ in composition depending on xyloside and cell type, detailed knowledge regarding a structure-function relationship is lacking.
27226567	1	18	theme	xyloside	185:192	arg1	XylNapOH					236:243	XylNapOH	236:243	XylNapOH	236:243	We previously reported that the xyloside 2-(6-hydroxynaphthyl) β-d-xylopyranoside (XylNapOH), in contrast to 2-naphthyl β-d-xylopyranoside (XylNap), specifically reduces tumor growth both in vitro and in vivo Although there are indications that this could be mediated by the xyloside-primed glycosaminoglycans (GAGs) and that these differ in composition depending on xyloside and cell type, detailed knowledge regarding a structure-function relationship is lacking.
27226567	0	19	theme	Cytotoxic	125:133	arg1	Effects					135:141	Cytotoxic Effects	125:141	Cytotoxic Effects	125:141	Xyloside-primed Chondroitin Sulfate/Dermatan Sulfate from Breast Carcinoma Cells with a Defined Disaccharide Composition Has Cytotoxic Effects in Vitro.
27226567	1	20	dep	reduces	315:321	arg1	reported					167:174	reported	167:174	reported that the xyloside 2-(6-hydroxynaphthyl) β-d-xylopyranoside (XylNapOH)	167:244	We previously reported that the xyloside 2-(6-hydroxynaphthyl) β-d-xylopyranoside (XylNapOH), in contrast to 2-naphthyl β-d-xylopyranoside (XylNap), specifically reduces tumor growth both in vitro and in vivo Although there are indications that this could be mediated by the xyloside-primed glycosaminoglycans (GAGs) and that these differ in composition depending on xyloside and cell type, detailed knowledge regarding a structure-function relationship is lacking.
27226567	4	21	contain	had	1262:1264	arg2	effect					1270:1275	any effect	1266:1275	any effect	1266:1275	In contrast, neither the chondroitin sulfate/dermatan sulfate nor the heparan sulfate derived from CCD-1095Sk cells primed on XylNapOH or XylNap had any effect on the growth of HCC70 cells or CCD-105Sk cells.
27226567	4	21	contain	had	1262:1264	arg1	sulfate					1171:1177	the chondroitin sulfate/dermatan sulfate	1138:1177	the chondroitin sulfate/dermatan sulfate	1138:1177	In contrast, neither the chondroitin sulfate/dermatan sulfate nor the heparan sulfate derived from CCD-1095Sk cells primed on XylNapOH or XylNap had any effect on the growth of HCC70 cells or CCD-105Sk cells.
27226567	4	21	contain	had	1262:1264	arg1	sulfate					1195:1201	the heparan sulfate	1183:1201	the heparan sulfate derived from CCD-1095Sk cells primed on XylNapOH or XylNap	1183:1260	In contrast, neither the chondroitin sulfate/dermatan sulfate nor the heparan sulfate derived from CCD-1095Sk cells primed on XylNapOH or XylNap had any effect on the growth of HCC70 cells or CCD-105Sk cells.
27226567	2	22	theme	carcinoma	692:700	arg1	line					707:710	a breast carcinoma cell line	683:710	a breast carcinoma cell line	683:710	In this study we isolated XylNapOH- and XylNap-primed GAGs from a breast carcinoma cell line, HCC70, and a breast fibroblast cell line, CCD-1095Sk, and demonstrated that both XylNapOH- and XylNap-primed chondroitin sulfate/dermatan sulfate GAGs derived from HCC70 cells had a cytotoxic effect on HCC70 cells and CCD-1095Sk cells.
27226567	1	23	theme	detailed	544:551	arg1	knowledge					553:561	detailed knowledge	544:561	detailed knowledge regarding a structure-function relationship	544:605	We previously reported that the xyloside 2-(6-hydroxynaphthyl) β-d-xylopyranoside (XylNapOH), in contrast to 2-naphthyl β-d-xylopyranoside (XylNap), specifically reduces tumor growth both in vitro and in vivo Although there are indications that this could be mediated by the xyloside-primed glycosaminoglycans (GAGs) and that these differ in composition depending on xyloside and cell type, detailed knowledge regarding a structure-function relationship is lacking.
27226567	4	24	theme	cells	1319:1323	arg1	growth					1284:1289	the growth	1280:1289	the growth of HCC70 cells or CCD-105Sk cells	1280:1323	In contrast, neither the chondroitin sulfate/dermatan sulfate nor the heparan sulfate derived from CCD-1095Sk cells primed on XylNapOH or XylNap had any effect on the growth of HCC70 cells or CCD-105Sk cells.
27226567	0	25	theme	Chondroitin	16:26	arg1	Sulfate					45:51	Xyloside-primed Chondroitin Sulfate/Dermatan Sulfate	0:51	Xyloside-primed Chondroitin Sulfate/Dermatan Sulfate from Breast Carcinoma Cells with a Defined Disaccharide Composition	0:119	Xyloside-primed Chondroitin Sulfate/Dermatan Sulfate from Breast Carcinoma Cells with a Defined Disaccharide Composition Has Cytotoxic Effects in Vitro.
27226567	3	26	theme	XylNap-primed	1081:1093	arg1	GAGs					1111:1114	the XylNap-primed heparan sulfate GAGs	1077:1114	the XylNap-primed heparan sulfate GAGs	1077:1114	The cytotoxic effect appeared to be mediated by induction of apoptosis and was inhibited in a concentration-dependent manner by the XylNap-primed heparan sulfate GAGs.
27226567	2	27	theme	breast	685:690	arg1	line					707:710	a breast carcinoma cell line	683:710	a breast carcinoma cell line	683:710	In this study we isolated XylNapOH- and XylNap-primed GAGs from a breast carcinoma cell line, HCC70, and a breast fibroblast cell line, CCD-1095Sk, and demonstrated that both XylNapOH- and XylNap-primed chondroitin sulfate/dermatan sulfate GAGs derived from HCC70 cells had a cytotoxic effect on HCC70 cells and CCD-1095Sk cells.
27226567	2	28	theme	fibroblast	733:742	arg1	line					749:752	a breast fibroblast cell line	724:752	a breast fibroblast cell line	724:752	In this study we isolated XylNapOH- and XylNap-primed GAGs from a breast carcinoma cell line, HCC70, and a breast fibroblast cell line, CCD-1095Sk, and demonstrated that both XylNapOH- and XylNap-primed chondroitin sulfate/dermatan sulfate GAGs derived from HCC70 cells had a cytotoxic effect on HCC70 cells and CCD-1095Sk cells.
27226567	2	28	theme	fibroblast	733:742	arg1	CCD-1095Sk					755:764	CCD-1095Sk	755:764	CCD-1095Sk	755:764	In this study we isolated XylNapOH- and XylNap-primed GAGs from a breast carcinoma cell line, HCC70, and a breast fibroblast cell line, CCD-1095Sk, and demonstrated that both XylNapOH- and XylNap-primed chondroitin sulfate/dermatan sulfate GAGs derived from HCC70 cells had a cytotoxic effect on HCC70 cells and CCD-1095Sk cells.
27226567	0	29	theme	Xyloside-primed	0:14	arg1	Sulfate					45:51	Xyloside-primed Chondroitin Sulfate/Dermatan Sulfate	0:51	Xyloside-primed Chondroitin Sulfate/Dermatan Sulfate from Breast Carcinoma Cells with a Defined Disaccharide Composition	0:119	Xyloside-primed Chondroitin Sulfate/Dermatan Sulfate from Breast Carcinoma Cells with a Defined Disaccharide Composition Has Cytotoxic Effects in Vitro.
27226567	3	30	theme	heparan	1095:1101	arg1	GAGs					1111:1114	the XylNap-primed heparan sulfate GAGs	1077:1114	the XylNap-primed heparan sulfate GAGs	1077:1114	The cytotoxic effect appeared to be mediated by induction of apoptosis and was inhibited in a concentration-dependent manner by the XylNap-primed heparan sulfate GAGs.
27226567	1	31	theme	6-hydroxynaphthyl	197:213	arg1	β-d-xylopyranoside					216:233	the xyloside 2-(6-hydroxynaphthyl) β-d-xylopyranoside	181:233	the xyloside 2-(6-hydroxynaphthyl) β-d-xylopyranoside (XylNapOH)	181:244	We previously reported that the xyloside 2-(6-hydroxynaphthyl) β-d-xylopyranoside (XylNapOH), in contrast to 2-naphthyl β-d-xylopyranoside (XylNap), specifically reduces tumor growth both in vitro and in vivo Although there are indications that this could be mediated by the xyloside-primed glycosaminoglycans (GAGs) and that these differ in composition depending on xyloside and cell type, detailed knowledge regarding a structure-function relationship is lacking.
27226567	1	31	theme	6-hydroxynaphthyl	197:213	arg1	XylNapOH					236:243	XylNapOH	236:243	XylNapOH	236:243	We previously reported that the xyloside 2-(6-hydroxynaphthyl) β-d-xylopyranoside (XylNapOH), in contrast to 2-naphthyl β-d-xylopyranoside (XylNap), specifically reduces tumor growth both in vitro and in vivo Although there are indications that this could be mediated by the xyloside-primed glycosaminoglycans (GAGs) and that these differ in composition depending on xyloside and cell type, detailed knowledge regarding a structure-function relationship is lacking.
27226567	2	32	theme	HCC70	915:919	arg1	cells					921:925	HCC70 cells	915:925	HCC70 cells	915:925	In this study we isolated XylNapOH- and XylNap-primed GAGs from a breast carcinoma cell line, HCC70, and a breast fibroblast cell line, CCD-1095Sk, and demonstrated that both XylNapOH- and XylNap-primed chondroitin sulfate/dermatan sulfate GAGs derived from HCC70 cells had a cytotoxic effect on HCC70 cells and CCD-1095Sk cells.
27226567	5	33	theme	cell	1530:1533	arg1	line					1535:1538	the same cell line	1521:1538	the same cell line	1521:1538	These observations were related to the disaccharide composition of the XylNapOH- and XylNap-primed GAGs, which differed between the two cell lines but was similar when the GAGs were derived from the same cell line.
27226567	6	34	theme	chondroitin	1616:1626	arg1	sulfate					1645:1651	chondroitin sulfate/dermatan sulfate	1616:1651	chondroitin sulfate/dermatan sulfate	1616:1651	To our knowledge this is the first report on cytotoxic effects mediated by chondroitin sulfate/dermatan sulfate.
27226567	3	35	theme	apoptosis	1010:1018	arg1	induction					997:1005	induction	997:1005	induction of apoptosis	997:1018	The cytotoxic effect appeared to be mediated by induction of apoptosis and was inhibited in a concentration-dependent manner by the XylNap-primed heparan sulfate GAGs.
27226567	0	36	theme	Sulfate/Dermatan	28:43	arg1	Sulfate					45:51	Xyloside-primed Chondroitin Sulfate/Dermatan Sulfate	0:51	Xyloside-primed Chondroitin Sulfate/Dermatan Sulfate from Breast Carcinoma Cells with a Defined Disaccharide Composition	0:119	Xyloside-primed Chondroitin Sulfate/Dermatan Sulfate from Breast Carcinoma Cells with a Defined Disaccharide Composition Has Cytotoxic Effects in Vitro.
27226567	6	37	theme	first	1570:1574	arg1	this					1558:1561	this	1558:1561	this	1558:1561	To our knowledge this is the first report on cytotoxic effects mediated by chondroitin sulfate/dermatan sulfate.
27226567	6	37	theme	first	1570:1574	arg1	report					1576:1581	the first report	1566:1581	the first report on cytotoxic effects mediated by chondroitin sulfate/dermatan sulfate	1566:1651	To our knowledge this is the first report on cytotoxic effects mediated by chondroitin sulfate/dermatan sulfate.
27226567	4	38	theme	HCC70	1294:1298	arg1	cells					1300:1304	HCC70 cells	1294:1304	HCC70 cells	1294:1304	In contrast, neither the chondroitin sulfate/dermatan sulfate nor the heparan sulfate derived from CCD-1095Sk cells primed on XylNapOH or XylNap had any effect on the growth of HCC70 cells or CCD-105Sk cells.
27226567	1	39	theme	structure-function	575:592	arg1	relationship					594:605	a structure-function relationship	573:605	a structure-function relationship	573:605	We previously reported that the xyloside 2-(6-hydroxynaphthyl) β-d-xylopyranoside (XylNapOH), in contrast to 2-naphthyl β-d-xylopyranoside (XylNap), specifically reduces tumor growth both in vitro and in vivo Although there are indications that this could be mediated by the xyloside-primed glycosaminoglycans (GAGs) and that these differ in composition depending on xyloside and cell type, detailed knowledge regarding a structure-function relationship is lacking.
27226567	0	40	theme	Breast	58:63	arg1	Cells					75:79	Breast Carcinoma Cells	58:79	Breast Carcinoma Cells	58:79	Xyloside-primed Chondroitin Sulfate/Dermatan Sulfate from Breast Carcinoma Cells with a Defined Disaccharide Composition Has Cytotoxic Effects in Vitro.
27226567	5	41	theme	disaccharide	1365:1376	arg1	composition					1378:1388	the disaccharide composition	1361:1388	the disaccharide composition of the XylNapOH- and XylNap-primed GAGs, which differed between the two cell lines but was similar when the GAGs were derived from the same cell line	1361:1538	These observations were related to the disaccharide composition of the XylNapOH- and XylNap-primed GAGs, which differed between the two cell lines but was similar when the GAGs were derived from the same cell line.
27226567	5	42	theme	same	1525:1528	arg1	line					1535:1538	the same cell line	1521:1538	the same cell line	1521:1538	These observations were related to the disaccharide composition of the XylNapOH- and XylNap-primed GAGs, which differed between the two cell lines but was similar when the GAGs were derived from the same cell line.
27226567	0	43	with	Sulfate	45:51	arg1	Composition					109:119	a Defined Disaccharide Composition	86:119	a Defined Disaccharide Composition	86:119	Xyloside-primed Chondroitin Sulfate/Dermatan Sulfate from Breast Carcinoma Cells with a Defined Disaccharide Composition Has Cytotoxic Effects in Vitro.
27226567	6	44	from	report	1576:1581	arg1	effects					1596:1602	cytotoxic effects	1586:1602	cytotoxic effects mediated by chondroitin sulfate/dermatan sulfate	1586:1651	To our knowledge this is the first report on cytotoxic effects mediated by chondroitin sulfate/dermatan sulfate.
27226567	1	45	theme	tumor	323:327	arg1	growth					329:334	tumor growth	323:334	tumor growth	323:334	We previously reported that the xyloside 2-(6-hydroxynaphthyl) β-d-xylopyranoside (XylNapOH), in contrast to 2-naphthyl β-d-xylopyranoside (XylNap), specifically reduces tumor growth both in vitro and in vivo Although there are indications that this could be mediated by the xyloside-primed glycosaminoglycans (GAGs) and that these differ in composition depending on xyloside and cell type, detailed knowledge regarding a structure-function relationship is lacking.
27226567	3	46	theme	cytotoxic	953:961	arg1	effect					963:968	The cytotoxic effect	949:968	The cytotoxic effect	949:968	The cytotoxic effect appeared to be mediated by induction of apoptosis and was inhibited in a concentration-dependent manner by the XylNap-primed heparan sulfate GAGs.
27226567	5	47	theme	XylNapOH-	1397:1405	arg1	GAGs					1425:1428	the XylNapOH- and XylNap-primed GAGs	1393:1428	the XylNapOH- and XylNap-primed GAGs	1393:1428	These observations were related to the disaccharide composition of the XylNapOH- and XylNap-primed GAGs, which differed between the two cell lines but was similar when the GAGs were derived from the same cell line.
27226567	5	47	theme	XylNapOH-	1397:1405	arg1	similar					1481:1487	similar	1481:1487	similar	1481:1487	These observations were related to the disaccharide composition of the XylNapOH- and XylNap-primed GAGs, which differed between the two cell lines but was similar when the GAGs were derived from the same cell line.
27226567	0	48	theme	Carcinoma	65:73	arg1	Cells					75:79	Breast Carcinoma Cells	58:79	Breast Carcinoma Cells	58:79	Xyloside-primed Chondroitin Sulfate/Dermatan Sulfate from Breast Carcinoma Cells with a Defined Disaccharide Composition Has Cytotoxic Effects in Vitro.
27226567	2	49	theme	CCD-1095Sk	931:940	arg1	cells					942:946	CCD-1095Sk cells	931:946	CCD-1095Sk cells	931:946	In this study we isolated XylNapOH- and XylNap-primed GAGs from a breast carcinoma cell line, HCC70, and a breast fibroblast cell line, CCD-1095Sk, and demonstrated that both XylNapOH- and XylNap-primed chondroitin sulfate/dermatan sulfate GAGs derived from HCC70 cells had a cytotoxic effect on HCC70 cells and CCD-1095Sk cells.
27226567	1	50	dep	β-d-xylopyranoside	273:290	arg1	contrast					250:257	contrast	250:257	contrast	250:257	We previously reported that the xyloside 2-(6-hydroxynaphthyl) β-d-xylopyranoside (XylNapOH), in contrast to 2-naphthyl β-d-xylopyranoside (XylNap), specifically reduces tumor growth both in vitro and in vivo Although there are indications that this could be mediated by the xyloside-primed glycosaminoglycans (GAGs) and that these differ in composition depending on xyloside and cell type, detailed knowledge regarding a structure-function relationship is lacking.
27226567	4	51	theme	CCD-1095Sk	1216:1225	arg1	cells					1227:1231	CCD-1095Sk cells	1216:1231	CCD-1095Sk cells primed on XylNapOH or XylNap	1216:1260	In contrast, neither the chondroitin sulfate/dermatan sulfate nor the heparan sulfate derived from CCD-1095Sk cells primed on XylNapOH or XylNap had any effect on the growth of HCC70 cells or CCD-105Sk cells.
27226567	6	52	theme	cytotoxic	1586:1594	arg1	effects					1596:1602	cytotoxic effects	1586:1602	cytotoxic effects mediated by chondroitin sulfate/dermatan sulfate	1586:1651	To our knowledge this is the first report on cytotoxic effects mediated by chondroitin sulfate/dermatan sulfate.
27226567	5	53	theme	GAGs	1425:1428	arg1	composition					1378:1388	the disaccharide composition	1361:1388	the disaccharide composition of the XylNapOH- and XylNap-primed GAGs, which differed between the two cell lines but was similar when the GAGs were derived from the same cell line	1361:1538	These observations were related to the disaccharide composition of the XylNapOH- and XylNap-primed GAGs, which differed between the two cell lines but was similar when the GAGs were derived from the same cell line.
27226567	0	54	contain	Has	121:123	arg2	Effects					135:141	Cytotoxic Effects	125:141	Cytotoxic Effects	125:141	Xyloside-primed Chondroitin Sulfate/Dermatan Sulfate from Breast Carcinoma Cells with a Defined Disaccharide Composition Has Cytotoxic Effects in Vitro.
27226567	0	54	contain	Has	121:123	arg1	Sulfate					45:51	Xyloside-primed Chondroitin Sulfate/Dermatan Sulfate	0:51	Xyloside-primed Chondroitin Sulfate/Dermatan Sulfate from Breast Carcinoma Cells with a Defined Disaccharide Composition	0:119	Xyloside-primed Chondroitin Sulfate/Dermatan Sulfate from Breast Carcinoma Cells with a Defined Disaccharide Composition Has Cytotoxic Effects in Vitro.
27226567	2	55	theme	XylNapOH-	794:802	arg1	GAGs					859:862	both XylNapOH- and XylNap-primed chondroitin sulfate/dermatan sulfate GAGs	789:862	both XylNapOH- and XylNap-primed chondroitin sulfate/dermatan sulfate GAGs derived from HCC70 cells	789:887	In this study we isolated XylNapOH- and XylNap-primed GAGs from a breast carcinoma cell line, HCC70, and a breast fibroblast cell line, CCD-1095Sk, and demonstrated that both XylNapOH- and XylNap-primed chondroitin sulfate/dermatan sulfate GAGs derived from HCC70 cells had a cytotoxic effect on HCC70 cells and CCD-1095Sk cells.
27226567	2	56	theme	HCC70	877:881	arg1	cells					883:887	HCC70 cells	877:887	HCC70 cells	877:887	In this study we isolated XylNapOH- and XylNap-primed GAGs from a breast carcinoma cell line, HCC70, and a breast fibroblast cell line, CCD-1095Sk, and demonstrated that both XylNapOH- and XylNap-primed chondroitin sulfate/dermatan sulfate GAGs derived from HCC70 cells had a cytotoxic effect on HCC70 cells and CCD-1095Sk cells.
27226567	2	57	theme	cell	702:705	arg1	line					707:710	a breast carcinoma cell line	683:710	a breast carcinoma cell line	683:710	In this study we isolated XylNapOH- and XylNap-primed GAGs from a breast carcinoma cell line, HCC70, and a breast fibroblast cell line, CCD-1095Sk, and demonstrated that both XylNapOH- and XylNap-primed chondroitin sulfate/dermatan sulfate GAGs derived from HCC70 cells had a cytotoxic effect on HCC70 cells and CCD-1095Sk cells.
27226567	2	58	contain	had	889:891	arg2	effect					905:910	a cytotoxic effect	893:910	a cytotoxic effect	893:910	In this study we isolated XylNapOH- and XylNap-primed GAGs from a breast carcinoma cell line, HCC70, and a breast fibroblast cell line, CCD-1095Sk, and demonstrated that both XylNapOH- and XylNap-primed chondroitin sulfate/dermatan sulfate GAGs derived from HCC70 cells had a cytotoxic effect on HCC70 cells and CCD-1095Sk cells.
27226567	2	58	contain	had	889:891	arg1	GAGs					859:862	both XylNapOH- and XylNap-primed chondroitin sulfate/dermatan sulfate GAGs	789:862	both XylNapOH- and XylNap-primed chondroitin sulfate/dermatan sulfate GAGs derived from HCC70 cells	789:887	In this study we isolated XylNapOH- and XylNap-primed GAGs from a breast carcinoma cell line, HCC70, and a breast fibroblast cell line, CCD-1095Sk, and demonstrated that both XylNapOH- and XylNap-primed chondroitin sulfate/dermatan sulfate GAGs derived from HCC70 cells had a cytotoxic effect on HCC70 cells and CCD-1095Sk cells.
27226567	2	59	theme	breast	726:731	arg1	line					749:752	a breast fibroblast cell line	724:752	a breast fibroblast cell line	724:752	In this study we isolated XylNapOH- and XylNap-primed GAGs from a breast carcinoma cell line, HCC70, and a breast fibroblast cell line, CCD-1095Sk, and demonstrated that both XylNapOH- and XylNap-primed chondroitin sulfate/dermatan sulfate GAGs derived from HCC70 cells had a cytotoxic effect on HCC70 cells and CCD-1095Sk cells.
27226567	2	59	theme	breast	726:731	arg1	CCD-1095Sk					755:764	CCD-1095Sk	755:764	CCD-1095Sk	755:764	In this study we isolated XylNapOH- and XylNap-primed GAGs from a breast carcinoma cell line, HCC70, and a breast fibroblast cell line, CCD-1095Sk, and demonstrated that both XylNapOH- and XylNap-primed chondroitin sulfate/dermatan sulfate GAGs derived from HCC70 cells had a cytotoxic effect on HCC70 cells and CCD-1095Sk cells.
27226567	4	60	theme	sulfate/dermatan	1154:1169	arg1	sulfate					1171:1177	the chondroitin sulfate/dermatan sulfate	1138:1177	the chondroitin sulfate/dermatan sulfate	1138:1177	In contrast, neither the chondroitin sulfate/dermatan sulfate nor the heparan sulfate derived from CCD-1095Sk cells primed on XylNapOH or XylNap had any effect on the growth of HCC70 cells or CCD-105Sk cells.
27226567	2	61	theme	cell	744:747	arg1	line					749:752	a breast fibroblast cell line	724:752	a breast fibroblast cell line	724:752	In this study we isolated XylNapOH- and XylNap-primed GAGs from a breast carcinoma cell line, HCC70, and a breast fibroblast cell line, CCD-1095Sk, and demonstrated that both XylNapOH- and XylNap-primed chondroitin sulfate/dermatan sulfate GAGs derived from HCC70 cells had a cytotoxic effect on HCC70 cells and CCD-1095Sk cells.
27226567	2	61	theme	cell	744:747	arg1	CCD-1095Sk					755:764	CCD-1095Sk	755:764	CCD-1095Sk	755:764	In this study we isolated XylNapOH- and XylNap-primed GAGs from a breast carcinoma cell line, HCC70, and a breast fibroblast cell line, CCD-1095Sk, and demonstrated that both XylNapOH- and XylNap-primed chondroitin sulfate/dermatan sulfate GAGs derived from HCC70 cells had a cytotoxic effect on HCC70 cells and CCD-1095Sk cells.
27226567	5	62	attach	derived	1508:1514	arg1	line					1535:1538	the same cell line	1521:1538	the same cell line	1521:1538	These observations were related to the disaccharide composition of the XylNapOH- and XylNap-primed GAGs, which differed between the two cell lines but was similar when the GAGs were derived from the same cell line.
27226567	5	62	attach	derived	1508:1514	arg2	GAGs					1498:1501	the GAGs	1494:1501	the GAGs	1494:1501	These observations were related to the disaccharide composition of the XylNapOH- and XylNap-primed GAGs, which differed between the two cell lines but was similar when the GAGs were derived from the same cell line.
27226567	1	63	dep	indications	381:391	arg1	mediated					412:419	mediated	412:419	could be mediated by the xyloside-primed glycosaminoglycans (GAGs)	403:468	We previously reported that the xyloside 2-(6-hydroxynaphthyl) β-d-xylopyranoside (XylNapOH), in contrast to 2-naphthyl β-d-xylopyranoside (XylNap), specifically reduces tumor growth both in vitro and in vivo Although there are indications that this could be mediated by the xyloside-primed glycosaminoglycans (GAGs) and that these differ in composition depending on xyloside and cell type, detailed knowledge regarding a structure-function relationship is lacking.
27226567	1	63	dep	indications	381:391	arg1	differ					485:490	differ	485:490	differ in composition depending on xyloside and cell type, detailed knowledge regarding a structure-function relationship is lacking	485:616	We previously reported that the xyloside 2-(6-hydroxynaphthyl) β-d-xylopyranoside (XylNapOH), in contrast to 2-naphthyl β-d-xylopyranoside (XylNap), specifically reduces tumor growth both in vitro and in vivo Although there are indications that this could be mediated by the xyloside-primed glycosaminoglycans (GAGs) and that these differ in composition depending on xyloside and cell type, detailed knowledge regarding a structure-function relationship is lacking.
27226567	4	64	attach	derived	1203:1209	arg1	cells					1227:1231	CCD-1095Sk cells	1216:1231	CCD-1095Sk cells primed on XylNapOH or XylNap	1216:1260	In contrast, neither the chondroitin sulfate/dermatan sulfate nor the heparan sulfate derived from CCD-1095Sk cells primed on XylNapOH or XylNap had any effect on the growth of HCC70 cells or CCD-105Sk cells.
27226567	4	64	attach	derived	1203:1209	arg2	sulfate					1195:1201	the heparan sulfate	1183:1201	the heparan sulfate derived from CCD-1095Sk cells primed on XylNapOH or XylNap	1183:1260	In contrast, neither the chondroitin sulfate/dermatan sulfate nor the heparan sulfate derived from CCD-1095Sk cells primed on XylNapOH or XylNap had any effect on the growth of HCC70 cells or CCD-105Sk cells.
27226567	2	65	attach	derived	864:870	arg2	GAGs					859:862	both XylNapOH- and XylNap-primed chondroitin sulfate/dermatan sulfate GAGs	789:862	both XylNapOH- and XylNap-primed chondroitin sulfate/dermatan sulfate GAGs derived from HCC70 cells	789:887	In this study we isolated XylNapOH- and XylNap-primed GAGs from a breast carcinoma cell line, HCC70, and a breast fibroblast cell line, CCD-1095Sk, and demonstrated that both XylNapOH- and XylNap-primed chondroitin sulfate/dermatan sulfate GAGs derived from HCC70 cells had a cytotoxic effect on HCC70 cells and CCD-1095Sk cells.
27226567	2	65	attach	derived	864:870	arg1	cells					883:887	HCC70 cells	877:887	HCC70 cells	877:887	In this study we isolated XylNapOH- and XylNap-primed GAGs from a breast carcinoma cell line, HCC70, and a breast fibroblast cell line, CCD-1095Sk, and demonstrated that both XylNapOH- and XylNap-primed chondroitin sulfate/dermatan sulfate GAGs derived from HCC70 cells had a cytotoxic effect on HCC70 cells and CCD-1095Sk cells.
27226567	4	66	theme	heparan	1187:1193	arg1	sulfate					1195:1201	the heparan sulfate	1183:1201	the heparan sulfate derived from CCD-1095Sk cells primed on XylNapOH or XylNap	1183:1260	In contrast, neither the chondroitin sulfate/dermatan sulfate nor the heparan sulfate derived from CCD-1095Sk cells primed on XylNapOH or XylNap had any effect on the growth of HCC70 cells or CCD-105Sk cells.
27226567	2	67	theme	sulfate	851:857	arg1	GAGs					859:862	both XylNapOH- and XylNap-primed chondroitin sulfate/dermatan sulfate GAGs	789:862	both XylNapOH- and XylNap-primed chondroitin sulfate/dermatan sulfate GAGs derived from HCC70 cells	789:887	In this study we isolated XylNapOH- and XylNap-primed GAGs from a breast carcinoma cell line, HCC70, and a breast fibroblast cell line, CCD-1095Sk, and demonstrated that both XylNapOH- and XylNap-primed chondroitin sulfate/dermatan sulfate GAGs derived from HCC70 cells had a cytotoxic effect on HCC70 cells and CCD-1095Sk cells.
27226567	5	68	theme	XylNap-primed	1411:1423	arg1	GAGs					1425:1428	the XylNapOH- and XylNap-primed GAGs	1393:1428	the XylNapOH- and XylNap-primed GAGs	1393:1428	These observations were related to the disaccharide composition of the XylNapOH- and XylNap-primed GAGs, which differed between the two cell lines but was similar when the GAGs were derived from the same cell line.
27226567	5	68	theme	XylNap-primed	1411:1423	arg1	similar					1481:1487	similar	1481:1487	similar	1481:1487	These observations were related to the disaccharide composition of the XylNapOH- and XylNap-primed GAGs, which differed between the two cell lines but was similar when the GAGs were derived from the same cell line.
25131333	1	0	from	homeostasis	366:376	arg1	barrier					343:349	the intestinal barrier	328:349	the intestinal barrier	328:349	Gastrointestinal mucositis and infection by chemotherapy treatment are associated with alteration of intestinal microflora and bacterial translocation due to the potential damage induced by anti-cancer drugs on the intestinal barrier and microbiota homeostasis.
25131333	7	1	contain	have	1329:1332	arg2	component					1386:1394	a functional food component	1368:1394	a functional food component	1368:1394	Our results may have important implications for OBP as a functional food component or nutrient against chemotherapy induced intestinal injury and potential pathogenic intestinal disorders involving inflammation and infection.
25131333	7	1	contain	have	1329:1332	arg2	implications					1344:1355	important implications	1334:1355	important implications for OBP	1334:1363	Our results may have important implications for OBP as a functional food component or nutrient against chemotherapy induced intestinal injury and potential pathogenic intestinal disorders involving inflammation and infection.
25131333	7	1	contain	have	1329:1332	arg1	results					1317:1323	Our results	1313:1323	Our results	1313:1323	Our results may have important implications for OBP as a functional food component or nutrient against chemotherapy induced intestinal injury and potential pathogenic intestinal disorders involving inflammation and infection.
25131333	7	2	theme	chemotherapy	1416:1427	arg1	injury					1448:1453	chemotherapy induced intestinal injury	1416:1453	chemotherapy induced intestinal injury	1416:1453	Our results may have important implications for OBP as a functional food component or nutrient against chemotherapy induced intestinal injury and potential pathogenic intestinal disorders involving inflammation and infection.
25131333	5	3	theme	intestinal	932:941	arg1	rDNA					956:959	intestinal bacteria 16S rDNA	932:959	intestinal bacteria 16S rDNA	932:959	The clone libraries of intestinal bacteria 16S rDNA were used to decipher the difference in bacterial community structures in different groups of mice.
25131333	5	4	from	difference	987:996	arg1	mice					1055:1058	mice	1055:1058	mice	1055:1058	The clone libraries of intestinal bacteria 16S rDNA were used to decipher the difference in bacterial community structures in different groups of mice.
25131333	5	4	from	difference	987:996	arg1	structures					1021:1030	bacterial community structures	1001:1030	bacterial community structures	1001:1030	The clone libraries of intestinal bacteria 16S rDNA were used to decipher the difference in bacterial community structures in different groups of mice.
25131333	5	4	from	difference	987:996	arg1	groups					1045:1050	different groups	1035:1050	different groups of mice	1035:1058	The clone libraries of intestinal bacteria 16S rDNA were used to decipher the difference in bacterial community structures in different groups of mice.
25131333	6	5	dep	changed	1137:1143	arg1	up-regulated					1239:1250	up-regulated	1239:1250	up-regulated	1239:1250	Followed by RFLP evaluation and OTU abundance analysis, they imply that OBP changed the intestinal microflora composition, in which the quantity of probiotic Bifidobacterium got up-regulated but Bacteroidetes decreased in mice undergoing chemotherapy.
25131333	6	5	dep	changed	1137:1143	arg1	decreased					1270:1278	decreased	1270:1278	decreased in mice undergoing chemotherapy	1270:1310	Followed by RFLP evaluation and OTU abundance analysis, they imply that OBP changed the intestinal microflora composition, in which the quantity of probiotic Bifidobacterium got up-regulated but Bacteroidetes decreased in mice undergoing chemotherapy.
25131333	6	6	theme	probiotic	1209:1217	arg1	got					1235:1237	probiotic Bifidobacterium got	1209:1237	probiotic Bifidobacterium got	1209:1237	Followed by RFLP evaluation and OTU abundance analysis, they imply that OBP changed the intestinal microflora composition, in which the quantity of probiotic Bifidobacterium got up-regulated but Bacteroidetes decreased in mice undergoing chemotherapy.
25131333	6	7	theme	RFLP	1073:1076	arg1	evaluation					1078:1087	RFLP evaluation	1073:1087	RFLP evaluation	1073:1087	Followed by RFLP evaluation and OTU abundance analysis, they imply that OBP changed the intestinal microflora composition, in which the quantity of probiotic Bifidobacterium got up-regulated but Bacteroidetes decreased in mice undergoing chemotherapy.
25131333	1	8	theme	anti-cancer	307:317	arg1	drugs					319:323	anti-cancer drugs	307:323	anti-cancer drugs	307:323	Gastrointestinal mucositis and infection by chemotherapy treatment are associated with alteration of intestinal microflora and bacterial translocation due to the potential damage induced by anti-cancer drugs on the intestinal barrier and microbiota homeostasis.
25131333	7	9	dep	chemotherapy	1416:1427	arg1	induced					1429:1435	induced	1429:1435	induced	1429:1435	Our results may have important implications for OBP as a functional food component or nutrient against chemotherapy induced intestinal injury and potential pathogenic intestinal disorders involving inflammation and infection.
25131333	3	10	theme	mouse	555:559	arg1	model					561:565	a mouse model	553:565	a mouse model intraperitoneally injected with 50 mg kg(-1) of cyclophosphamide (Cy) for 2 days	553:646	In the current contribution, with a mouse model intraperitoneally injected with 50 mg kg(-1) of cyclophosphamide (Cy) for 2 days, we revealed that polysaccharides from the ink of Ommastrephes bartrami (OBP) altered the intestinal microflora composition.
25131333	6	11	theme	microflora	1160:1169	arg1	composition					1171:1181	the intestinal microflora composition	1145:1181	the intestinal microflora composition	1145:1181	Followed by RFLP evaluation and OTU abundance analysis, they imply that OBP changed the intestinal microflora composition, in which the quantity of probiotic Bifidobacterium got up-regulated but Bacteroidetes decreased in mice undergoing chemotherapy.
25131333	2	12	theme	intestinal	484:493	arg1	dysfunction					506:516	chemotherapy induced intestinal microflora dysfunction	463:516	chemotherapy induced intestinal microflora dysfunction	463:516	This study aimed to investigate the protective effect of dietary polysaccharides on chemotherapy induced intestinal microflora dysfunction.
25131333	4	13	theme	bacteria	821:828	arg1	growth					800:805	the excessive growth	786:805	the excessive growth of intestinal bacteria induced by cyclophosphamide	786:856	OBP retarded the excessive growth of intestinal bacteria induced by cyclophosphamide, based on 16S rRNA gene (16S rDNA) quantification.
25131333	2	14	theme	dietary	436:442	arg1	polysaccharides					444:458	dietary polysaccharides	436:458	dietary polysaccharides	436:458	This study aimed to investigate the protective effect of dietary polysaccharides on chemotherapy induced intestinal microflora dysfunction.
25131333	7	15	theme	food	1381:1384	arg1	implications					1344:1355	important implications	1334:1355	important implications for OBP	1334:1363	Our results may have important implications for OBP as a functional food component or nutrient against chemotherapy induced intestinal injury and potential pathogenic intestinal disorders involving inflammation and infection.
25131333	7	15	theme	food	1381:1384	arg1	component					1386:1394	a functional food component	1368:1394	a functional food component	1368:1394	Our results may have important implications for OBP as a functional food component or nutrient against chemotherapy induced intestinal injury and potential pathogenic intestinal disorders involving inflammation and infection.
25131333	3	16	from	ink	691:693	arg1	polysaccharides					666:680	polysaccharides	666:680	polysaccharides from the ink of Ommastrephes bartrami (OBP)	666:724	In the current contribution, with a mouse model intraperitoneally injected with 50 mg kg(-1) of cyclophosphamide (Cy) for 2 days, we revealed that polysaccharides from the ink of Ommastrephes bartrami (OBP) altered the intestinal microflora composition.
25131333	3	17	theme	intestinal	738:747	arg1	microflora					749:758	the intestinal microflora	734:758	the intestinal microflora composition	734:770	In the current contribution, with a mouse model intraperitoneally injected with 50 mg kg(-1) of cyclophosphamide (Cy) for 2 days, we revealed that polysaccharides from the ink of Ommastrephes bartrami (OBP) altered the intestinal microflora composition.
25131333	1	18	from	barrier	343:349	arg1	alteration					204:213	alteration	204:213	alteration of intestinal microflora and bacterial translocation due to the potential damage induced by anti-cancer drugs on the intestinal barrier	204:349	Gastrointestinal mucositis and infection by chemotherapy treatment are associated with alteration of intestinal microflora and bacterial translocation due to the potential damage induced by anti-cancer drugs on the intestinal barrier and microbiota homeostasis.
25131333	1	18	from	barrier	343:349	arg1	homeostasis					366:376	microbiota homeostasis	355:376	microbiota homeostasis	355:376	Gastrointestinal mucositis and infection by chemotherapy treatment are associated with alteration of intestinal microflora and bacterial translocation due to the potential damage induced by anti-cancer drugs on the intestinal barrier and microbiota homeostasis.
25131333	4	19	theme	intestinal	810:819	arg1	bacteria					821:828	intestinal bacteria	810:828	intestinal bacteria induced by cyclophosphamide	810:856	OBP retarded the excessive growth of intestinal bacteria induced by cyclophosphamide, based on 16S rRNA gene (16S rDNA) quantification.
25131333	2	20	theme	polysaccharides	444:458	arg1	effect					426:431	the protective effect	411:431	the protective effect of dietary polysaccharides on chemotherapy induced intestinal microflora dysfunction	411:516	This study aimed to investigate the protective effect of dietary polysaccharides on chemotherapy induced intestinal microflora dysfunction.
25131333	0	21	theme	squid	8:12	arg1	polysaccharides					18:32	Dietary squid ink polysaccharides	0:32	Dietary squid ink polysaccharides	0:32	Dietary squid ink polysaccharides ameliorated the intestinal microflora dysfunction in mice undergoing chemotherapy.
25131333	3	22	theme	microflora	749:758	arg1	composition					760:770	the intestinal microflora composition	734:770	the intestinal microflora composition	734:770	In the current contribution, with a mouse model intraperitoneally injected with 50 mg kg(-1) of cyclophosphamide (Cy) for 2 days, we revealed that polysaccharides from the ink of Ommastrephes bartrami (OBP) altered the intestinal microflora composition.
25131333	7	23	theme	intestinal	1480:1489	arg1	disorders					1491:1499	potential pathogenic intestinal disorders	1459:1499	potential pathogenic intestinal disorders involving inflammation and infection	1459:1536	Our results may have important implications for OBP as a functional food component or nutrient against chemotherapy induced intestinal injury and potential pathogenic intestinal disorders involving inflammation and infection.
25131333	1	24	theme	intestinal	218:227	arg1	microflora					229:238	intestinal microflora	218:238	intestinal microflora	218:238	Gastrointestinal mucositis and infection by chemotherapy treatment are associated with alteration of intestinal microflora and bacterial translocation due to the potential damage induced by anti-cancer drugs on the intestinal barrier and microbiota homeostasis.
25131333	0	25	theme	Dietary	0:6	arg1	polysaccharides					18:32	Dietary squid ink polysaccharides	0:32	Dietary squid ink polysaccharides	0:32	Dietary squid ink polysaccharides ameliorated the intestinal microflora dysfunction in mice undergoing chemotherapy.
25131333	2	26	from	effect	426:431	arg1	dysfunction					506:516	chemotherapy induced intestinal microflora dysfunction	463:516	chemotherapy induced intestinal microflora dysfunction	463:516	This study aimed to investigate the protective effect of dietary polysaccharides on chemotherapy induced intestinal microflora dysfunction.
25131333	7	27	theme	intestinal	1437:1446	arg1	injury					1448:1453	chemotherapy induced intestinal injury	1416:1453	chemotherapy induced intestinal injury	1416:1453	Our results may have important implications for OBP as a functional food component or nutrient against chemotherapy induced intestinal injury and potential pathogenic intestinal disorders involving inflammation and infection.
25131333	1	28	theme	microflora	229:238	arg1	alteration					204:213	alteration	204:213	alteration of intestinal microflora and bacterial translocation due to the potential damage induced by anti-cancer drugs on the intestinal barrier	204:349	Gastrointestinal mucositis and infection by chemotherapy treatment are associated with alteration of intestinal microflora and bacterial translocation due to the potential damage induced by anti-cancer drugs on the intestinal barrier and microbiota homeostasis.
25131333	1	28	theme	microflora	229:238	arg1	homeostasis					366:376	microbiota homeostasis	355:376	microbiota homeostasis	355:376	Gastrointestinal mucositis and infection by chemotherapy treatment are associated with alteration of intestinal microflora and bacterial translocation due to the potential damage induced by anti-cancer drugs on the intestinal barrier and microbiota homeostasis.
25131333	6	29	theme	got	1235:1237	arg1	quantity					1197:1204	the quantity	1193:1204	the quantity of probiotic Bifidobacterium got	1193:1237	Followed by RFLP evaluation and OTU abundance analysis, they imply that OBP changed the intestinal microflora composition, in which the quantity of probiotic Bifidobacterium got up-regulated but Bacteroidetes decreased in mice undergoing chemotherapy.
25131333	4	30	theme	16S	868:870	arg1	rRNA					872:875	16S rRNA	868:875	16S rRNA gene (16S rDNA) quantification	868:906	OBP retarded the excessive growth of intestinal bacteria induced by cyclophosphamide, based on 16S rRNA gene (16S rDNA) quantification.
25131333	1	31	theme	Gastrointestinal	117:132	arg1	mucositis					134:142	Gastrointestinal mucositis	117:142	Gastrointestinal mucositis	117:142	Gastrointestinal mucositis and infection by chemotherapy treatment are associated with alteration of intestinal microflora and bacterial translocation due to the potential damage induced by anti-cancer drugs on the intestinal barrier and microbiota homeostasis.
25131333	2	32	theme	microflora	495:504	arg1	dysfunction					506:516	chemotherapy induced intestinal microflora dysfunction	463:516	chemotherapy induced intestinal microflora dysfunction	463:516	This study aimed to investigate the protective effect of dietary polysaccharides on chemotherapy induced intestinal microflora dysfunction.
25131333	0	33	theme	ink	14:16	arg1	polysaccharides					18:32	Dietary squid ink polysaccharides	0:32	Dietary squid ink polysaccharides	0:32	Dietary squid ink polysaccharides ameliorated the intestinal microflora dysfunction in mice undergoing chemotherapy.
25131333	1	34	theme	intestinal	332:341	arg1	barrier					343:349	the intestinal barrier	328:349	the intestinal barrier	328:349	Gastrointestinal mucositis and infection by chemotherapy treatment are associated with alteration of intestinal microflora and bacterial translocation due to the potential damage induced by anti-cancer drugs on the intestinal barrier and microbiota homeostasis.
25131333	5	35	theme	bacterial	1001:1009	arg1	structures					1021:1030	bacterial community structures	1001:1030	bacterial community structures	1001:1030	The clone libraries of intestinal bacteria 16S rDNA were used to decipher the difference in bacterial community structures in different groups of mice.
25131333	3	36	theme	cyclophosphamide	615:630	arg1	-1					608:609	-1	608:609	-1	608:609	In the current contribution, with a mouse model intraperitoneally injected with 50 mg kg(-1) of cyclophosphamide (Cy) for 2 days, we revealed that polysaccharides from the ink of Ommastrephes bartrami (OBP) altered the intestinal microflora composition.
25131333	3	36	theme	cyclophosphamide	615:630	arg1	mg					602:603	50 mg kg	599:606	50 mg kg(-1) of cyclophosphamide (Cy) for 2 days	599:646	In the current contribution, with a mouse model intraperitoneally injected with 50 mg kg(-1) of cyclophosphamide (Cy) for 2 days, we revealed that polysaccharides from the ink of Ommastrephes bartrami (OBP) altered the intestinal microflora composition.
25131333	1	37	theme	bacterial	244:252	arg1	translocation					254:266	bacterial translocation	244:266	bacterial translocation	244:266	Gastrointestinal mucositis and infection by chemotherapy treatment are associated with alteration of intestinal microflora and bacterial translocation due to the potential damage induced by anti-cancer drugs on the intestinal barrier and microbiota homeostasis.
25131333	7	38	theme	important	1334:1342	arg1	implications					1344:1355	important implications	1334:1355	important implications for OBP	1334:1363	Our results may have important implications for OBP as a functional food component or nutrient against chemotherapy induced intestinal injury and potential pathogenic intestinal disorders involving inflammation and infection.
25131333	7	38	theme	important	1334:1342	arg1	component					1386:1394	a functional food component	1368:1394	a functional food component	1368:1394	Our results may have important implications for OBP as a functional food component or nutrient against chemotherapy induced intestinal injury and potential pathogenic intestinal disorders involving inflammation and infection.
25131333	3	39	theme	current	526:532	arg1	contribution					534:545	the current contribution	522:545	the current contribution	522:545	In the current contribution, with a mouse model intraperitoneally injected with 50 mg kg(-1) of cyclophosphamide (Cy) for 2 days, we revealed that polysaccharides from the ink of Ommastrephes bartrami (OBP) altered the intestinal microflora composition.
25131333	5	40	used	used	966:969	arg2	libraries					919:927	The clone libraries	909:927	The clone libraries of intestinal bacteria 16S rDNA	909:959	The clone libraries of intestinal bacteria 16S rDNA were used to decipher the difference in bacterial community structures in different groups of mice.
25131333	1	41	theme	translocation	254:266	arg1	alteration					204:213	alteration	204:213	alteration of intestinal microflora and bacterial translocation due to the potential damage induced by anti-cancer drugs on the intestinal barrier	204:349	Gastrointestinal mucositis and infection by chemotherapy treatment are associated with alteration of intestinal microflora and bacterial translocation due to the potential damage induced by anti-cancer drugs on the intestinal barrier and microbiota homeostasis.
25131333	1	41	theme	translocation	254:266	arg1	homeostasis					366:376	microbiota homeostasis	355:376	microbiota homeostasis	355:376	Gastrointestinal mucositis and infection by chemotherapy treatment are associated with alteration of intestinal microflora and bacterial translocation due to the potential damage induced by anti-cancer drugs on the intestinal barrier and microbiota homeostasis.
25131333	6	42	theme	abundance	1097:1105	arg1	analysis					1107:1114	OTU abundance analysis	1093:1114	OTU abundance analysis	1093:1114	Followed by RFLP evaluation and OTU abundance analysis, they imply that OBP changed the intestinal microflora composition, in which the quantity of probiotic Bifidobacterium got up-regulated but Bacteroidetes decreased in mice undergoing chemotherapy.
25131333	7	43	theme	potential	1459:1467	arg1	disorders					1491:1499	potential pathogenic intestinal disorders	1459:1499	potential pathogenic intestinal disorders involving inflammation and infection	1459:1536	Our results may have important implications for OBP as a functional food component or nutrient against chemotherapy induced intestinal injury and potential pathogenic intestinal disorders involving inflammation and infection.
25131333	2	44	dep	chemotherapy	463:474	arg1	induced					476:482	induced	476:482	induced	476:482	This study aimed to investigate the protective effect of dietary polysaccharides on chemotherapy induced intestinal microflora dysfunction.
25131333	1	45	from	translocation	254:266	arg1	barrier					343:349	the intestinal barrier	328:349	the intestinal barrier	328:349	Gastrointestinal mucositis and infection by chemotherapy treatment are associated with alteration of intestinal microflora and bacterial translocation due to the potential damage induced by anti-cancer drugs on the intestinal barrier and microbiota homeostasis.
25131333	2	46	theme	chemotherapy	463:474	arg1	dysfunction					506:516	chemotherapy induced intestinal microflora dysfunction	463:516	chemotherapy induced intestinal microflora dysfunction	463:516	This study aimed to investigate the protective effect of dietary polysaccharides on chemotherapy induced intestinal microflora dysfunction.
25131333	0	47	theme	microflora	61:70	arg1	dysfunction					72:82	the intestinal microflora dysfunction	46:82	the intestinal microflora dysfunction in mice undergoing chemotherapy	46:114	Dietary squid ink polysaccharides ameliorated the intestinal microflora dysfunction in mice undergoing chemotherapy.
25131333	1	48	theme	microbiota	355:364	arg1	homeostasis					366:376	microbiota homeostasis	355:376	microbiota homeostasis	355:376	Gastrointestinal mucositis and infection by chemotherapy treatment are associated with alteration of intestinal microflora and bacterial translocation due to the potential damage induced by anti-cancer drugs on the intestinal barrier and microbiota homeostasis.
25131333	0	49	from	dysfunction	72:82	arg1	mice					87:90	mice	87:90	mice undergoing chemotherapy	87:114	Dietary squid ink polysaccharides ameliorated the intestinal microflora dysfunction in mice undergoing chemotherapy.
25131333	4	50	theme	rRNA	872:875	arg1	gene					877:880	16S rRNA gene	868:880	16S rRNA gene (16S rDNA) quantification	868:906	OBP retarded the excessive growth of intestinal bacteria induced by cyclophosphamide, based on 16S rRNA gene (16S rDNA) quantification.
25131333	4	50	theme	rRNA	872:875	arg1	rDNA					887:890	16S rDNA	883:890	16S rDNA	883:890	OBP retarded the excessive growth of intestinal bacteria induced by cyclophosphamide, based on 16S rRNA gene (16S rDNA) quantification.
25131333	5	51	theme	bacteria	943:950	arg1	rDNA					956:959	intestinal bacteria 16S rDNA	932:959	intestinal bacteria 16S rDNA	932:959	The clone libraries of intestinal bacteria 16S rDNA were used to decipher the difference in bacterial community structures in different groups of mice.
25131333	0	52	theme	intestinal	50:59	arg1	microflora					61:70	the intestinal microflora	46:70	the intestinal microflora dysfunction in mice undergoing chemotherapy	46:114	Dietary squid ink polysaccharides ameliorated the intestinal microflora dysfunction in mice undergoing chemotherapy.
25131333	7	53	theme	functional	1370:1379	arg1	implications					1344:1355	important implications	1334:1355	important implications for OBP	1334:1363	Our results may have important implications for OBP as a functional food component or nutrient against chemotherapy induced intestinal injury and potential pathogenic intestinal disorders involving inflammation and infection.
25131333	7	53	theme	functional	1370:1379	arg1	component					1386:1394	a functional food component	1368:1394	a functional food component	1368:1394	Our results may have important implications for OBP as a functional food component or nutrient against chemotherapy induced intestinal injury and potential pathogenic intestinal disorders involving inflammation and infection.
25131333	3	54	theme	bartrami	711:718	arg1	ink					691:693	the ink	687:693	the ink of Ommastrephes bartrami (OBP)	687:724	In the current contribution, with a mouse model intraperitoneally injected with 50 mg kg(-1) of cyclophosphamide (Cy) for 2 days, we revealed that polysaccharides from the ink of Ommastrephes bartrami (OBP) altered the intestinal microflora composition.
25131333	4	55	theme	gene	877:880	arg1	quantification					893:906	16S rRNA gene (16S rDNA) quantification	868:906	16S rRNA gene (16S rDNA) quantification	868:906	OBP retarded the excessive growth of intestinal bacteria induced by cyclophosphamide, based on 16S rRNA gene (16S rDNA) quantification.
25131333	6	56	theme	intestinal	1149:1158	arg1	microflora					1160:1169	the intestinal microflora	1145:1169	the intestinal microflora composition	1145:1181	Followed by RFLP evaluation and OTU abundance analysis, they imply that OBP changed the intestinal microflora composition, in which the quantity of probiotic Bifidobacterium got up-regulated but Bacteroidetes decreased in mice undergoing chemotherapy.
25131333	5	57	theme	mice	1055:1058	arg1	mice					1055:1058	mice	1055:1058	mice	1055:1058	The clone libraries of intestinal bacteria 16S rDNA were used to decipher the difference in bacterial community structures in different groups of mice.
25131333	5	57	theme	mice	1055:1058	arg1	groups					1045:1050	different groups	1035:1050	different groups of mice	1035:1058	The clone libraries of intestinal bacteria 16S rDNA were used to decipher the difference in bacterial community structures in different groups of mice.
25131333	5	58	theme	rDNA	956:959	arg1	libraries					919:927	The clone libraries	909:927	The clone libraries of intestinal bacteria 16S rDNA	909:959	The clone libraries of intestinal bacteria 16S rDNA were used to decipher the difference in bacterial community structures in different groups of mice.
25131333	4	59	theme	excessive	790:798	arg1	growth					800:805	the excessive growth	786:805	the excessive growth of intestinal bacteria induced by cyclophosphamide	786:856	OBP retarded the excessive growth of intestinal bacteria induced by cyclophosphamide, based on 16S rRNA gene (16S rDNA) quantification.
25131333	7	60	theme	pathogenic	1469:1478	arg1	disorders					1491:1499	potential pathogenic intestinal disorders	1459:1499	potential pathogenic intestinal disorders involving inflammation and infection	1459:1536	Our results may have important implications for OBP as a functional food component or nutrient against chemotherapy induced intestinal injury and potential pathogenic intestinal disorders involving inflammation and infection.
25131333	5	61	theme	community	1011:1019	arg1	structures					1021:1030	bacterial community structures	1001:1030	bacterial community structures	1001:1030	The clone libraries of intestinal bacteria 16S rDNA were used to decipher the difference in bacterial community structures in different groups of mice.
25131333	6	62	theme	OTU	1093:1095	arg1	analysis					1107:1114	OTU abundance analysis	1093:1114	OTU abundance analysis	1093:1114	Followed by RFLP evaluation and OTU abundance analysis, they imply that OBP changed the intestinal microflora composition, in which the quantity of probiotic Bifidobacterium got up-regulated but Bacteroidetes decreased in mice undergoing chemotherapy.
25131333	1	63	theme	chemotherapy	161:172	arg1	treatment					174:182	chemotherapy treatment	161:182	chemotherapy treatment	161:182	Gastrointestinal mucositis and infection by chemotherapy treatment are associated with alteration of intestinal microflora and bacterial translocation due to the potential damage induced by anti-cancer drugs on the intestinal barrier and microbiota homeostasis.
25131333	3	64	theme	Ommastrephes	698:709	arg1	OBP					721:723	OBP	721:723	OBP	721:723	In the current contribution, with a mouse model intraperitoneally injected with 50 mg kg(-1) of cyclophosphamide (Cy) for 2 days, we revealed that polysaccharides from the ink of Ommastrephes bartrami (OBP) altered the intestinal microflora composition.
25131333	3	64	theme	Ommastrephes	698:709	arg1	bartrami					711:718	Ommastrephes bartrami	698:718	Ommastrephes bartrami (OBP)	698:724	In the current contribution, with a mouse model intraperitoneally injected with 50 mg kg(-1) of cyclophosphamide (Cy) for 2 days, we revealed that polysaccharides from the ink of Ommastrephes bartrami (OBP) altered the intestinal microflora composition.
25131333	5	65	theme	different	1035:1043	arg1	mice					1055:1058	mice	1055:1058	mice	1055:1058	The clone libraries of intestinal bacteria 16S rDNA were used to decipher the difference in bacterial community structures in different groups of mice.
25131333	5	65	theme	different	1035:1043	arg1	groups					1045:1050	different groups	1035:1050	different groups of mice	1035:1058	The clone libraries of intestinal bacteria 16S rDNA were used to decipher the difference in bacterial community structures in different groups of mice.
25131333	1	66	from	microflora	229:238	arg1	barrier					343:349	the intestinal barrier	328:349	the intestinal barrier	328:349	Gastrointestinal mucositis and infection by chemotherapy treatment are associated with alteration of intestinal microflora and bacterial translocation due to the potential damage induced by anti-cancer drugs on the intestinal barrier and microbiota homeostasis.
25131333	5	67	theme	clone	913:917	arg1	libraries					919:927	The clone libraries	909:927	The clone libraries of intestinal bacteria 16S rDNA	909:959	The clone libraries of intestinal bacteria 16S rDNA were used to decipher the difference in bacterial community structures in different groups of mice.
25131333	1	68	theme	potential	279:287	arg1	damage					289:294	the potential damage	275:294	the potential damage induced by anti-cancer drugs	275:323	Gastrointestinal mucositis and infection by chemotherapy treatment are associated with alteration of intestinal microflora and bacterial translocation due to the potential damage induced by anti-cancer drugs on the intestinal barrier and microbiota homeostasis.
25131333	6	69	theme	Bifidobacterium	1219:1233	arg1	got					1235:1237	probiotic Bifidobacterium got	1209:1237	probiotic Bifidobacterium got	1209:1237	Followed by RFLP evaluation and OTU abundance analysis, they imply that OBP changed the intestinal microflora composition, in which the quantity of probiotic Bifidobacterium got up-regulated but Bacteroidetes decreased in mice undergoing chemotherapy.
25131333	1	70	from	alteration	204:213	arg1	barrier					343:349	the intestinal barrier	328:349	the intestinal barrier	328:349	Gastrointestinal mucositis and infection by chemotherapy treatment are associated with alteration of intestinal microflora and bacterial translocation due to the potential damage induced by anti-cancer drugs on the intestinal barrier and microbiota homeostasis.
25131333	2	71	theme	protective	415:424	arg1	effect					426:431	the protective effect	411:431	the protective effect of dietary polysaccharides on chemotherapy induced intestinal microflora dysfunction	411:516	This study aimed to investigate the protective effect of dietary polysaccharides on chemotherapy induced intestinal microflora dysfunction.
26137876	7	0	theme	roots	1322:1326	arg1	effects					1298:1304	the immunocompetence effects	1277:1304	the immunocompetence effects of adventitious roots (AR)	1277:1331	The delayed-type hypersensitivity (DTH) reaction on the mice induced by 2,4-dinitrofluorobenzene (DNFB) was applied to compare the immunocompetence effects of adventitious roots (AR) with field native roots (NR) of P. heterophylla.
26137876	4	1	theme	saponin	819:825	arg1	contents					801:808	the contents	797:808	the contents of total saponin, polysaccharides, and specific oxygen uptaker rate (SOUR)	797:883	Fed-batch cultivation enhanced the contents of total saponin, polysaccharides, and specific oxygen uptaker rate (SOUR).
26137876	8	2	contain	possessed	1398:1406	arg1	AR					1395:1396	AR	1395:1396	AR	1395:1396	As a result, AR possessed a similar immunoregulation function as NR.
26137876	8	2	contain	possessed	1398:1406	arg2	result					1387:1392	a result	1385:1392	a result	1385:1392	As a result, AR possessed a similar immunoregulation function as NR.
26137876	8	2	contain	possessed	1398:1406	arg2	function					1435:1442	a similar immunoregulation function	1408:1442	a similar immunoregulation function	1408:1442	As a result, AR possessed a similar immunoregulation function as NR.
26137876	7	3	theme	field	1338:1342	arg1	NR					1358:1359	NR	1358:1359	NR	1358:1359	The delayed-type hypersensitivity (DTH) reaction on the mice induced by 2,4-dinitrofluorobenzene (DNFB) was applied to compare the immunocompetence effects of adventitious roots (AR) with field native roots (NR) of P. heterophylla.
26137876	7	3	theme	field	1338:1342	arg1	roots					1351:1355	field native roots	1338:1355	field native roots (NR) of P. heterophylla	1338:1379	The delayed-type hypersensitivity (DTH) reaction on the mice induced by 2,4-dinitrofluorobenzene (DNFB) was applied to compare the immunocompetence effects of adventitious roots (AR) with field native roots (NR) of P. heterophylla.
26137876	2	4	theme	n	463:463	arg1	analysis					466:473	LC-MS(n) analysis	457:473	LC-MS(n) analysis	457:473	Pseudostellarin A, C, D, and G were identified from P. heterophylla adventitious roots on the basis of LC-MS(n) analysis.
26137876	4	5	theme	total	813:817	arg1	saponin					819:825	total saponin	813:825	total saponin	813:825	Fed-batch cultivation enhanced the contents of total saponin, polysaccharides, and specific oxygen uptaker rate (SOUR).
26137876	1	6	theme	electrospray	174:185	arg1	ESI-MS					224:229	ESI-MS	224:229	ESI-MS(n)	224:232	A electrospray ionization tandem mass spectrometry (ESI-MS(n)) analysis was performed in order to identify the active composition in Pseudostellaria heterophylla adventitious roots.
26137876	1	6	theme	electrospray	174:185	arg1	spectrometry					210:221	A electrospray ionization tandem mass spectrometry	172:221	A electrospray ionization tandem mass spectrometry (ESI-MS(n)) analysis	172:242	A electrospray ionization tandem mass spectrometry (ESI-MS(n)) analysis was performed in order to identify the active composition in Pseudostellaria heterophylla adventitious roots.
26137876	5	7	theme	Murashige	954:962	arg1	group					986:990	the 3/4 Murashige and Skoog (MS) medium group	946:990	the 3/4 Murashige and Skoog (MS) medium group	946:990	The maximum dry root weight (4.728 g l(-1)) was achieved in the 3/4 Murashige and Skoog (MS) medium group.
26137876	0	8	theme	Immune-Regulative	92:108	arg1	Effect					110:115	Immune-Regulative Effect	92:115	Immune-Regulative Effect of Adventitious Roots in Pseudostellaria heterophylla	92:169	HPLC-ESI-MS(n) Analysis, Fed-Batch Cultivation Enhances Bioactive Compound Biosynthesis and Immune-Regulative Effect of Adventitious Roots in Pseudostellaria heterophylla.
26137876	4	9	theme	polysaccharides	828:842	arg1	contents					801:808	the contents	797:808	the contents of total saponin, polysaccharides, and specific oxygen uptaker rate (SOUR)	797:883	Fed-batch cultivation enhanced the contents of total saponin, polysaccharides, and specific oxygen uptaker rate (SOUR).
26137876	5	10	theme	dry	898:900	arg1	l					923:923	4.728 g l(-1)	915:927	4.728 g l(-1)	915:927	The maximum dry root weight (4.728 g l(-1)) was achieved in the 3/4 Murashige and Skoog (MS) medium group.
26137876	5	10	theme	dry	898:900	arg1	weight					907:912	The maximum dry root weight	886:912	The maximum dry root weight (4.728 g l(-1))	886:928	The maximum dry root weight (4.728 g l(-1)) was achieved in the 3/4 Murashige and Skoog (MS) medium group.
26137876	3	11	theme	growth	637:642	arg1	ratio					644:648	the growth ratio	633:648	the growth ratio	633:648	The culture conditions of adventitious roots were optimized, and datasets were subjected to a partial least squares discriminant analysis (PLS-DA), in which the growth ratio and some compounds showed a positive correlation with an aeration volume of 0.3 vvm and inoculum density of 0.15 %.
26137876	3	12	theme	%	763:763	arg1	volume					716:721	an aeration volume	704:721	an aeration volume of 0.3 vvm	704:732	The culture conditions of adventitious roots were optimized, and datasets were subjected to a partial least squares discriminant analysis (PLS-DA), in which the growth ratio and some compounds showed a positive correlation with an aeration volume of 0.3 vvm and inoculum density of 0.15 %.
26137876	3	12	theme	%	763:763	arg1	density					747:753	inoculum density	738:753	inoculum density of 0.15 %	738:763	The culture conditions of adventitious roots were optimized, and datasets were subjected to a partial least squares discriminant analysis (PLS-DA), in which the growth ratio and some compounds showed a positive correlation with an aeration volume of 0.3 vvm and inoculum density of 0.15 %.
26137876	3	13	theme	positive	678:685	arg1	correlation					687:697	a positive correlation	676:697	a positive correlation with an aeration volume of 0.3 vvm and inoculum density of 0.15 %	676:763	The culture conditions of adventitious roots were optimized, and datasets were subjected to a partial least squares discriminant analysis (PLS-DA), in which the growth ratio and some compounds showed a positive correlation with an aeration volume of 0.3 vvm and inoculum density of 0.15 %.
26137876	5	14	theme	root	902:905	arg1	l					923:923	4.728 g l(-1)	915:927	4.728 g l(-1)	915:927	The maximum dry root weight (4.728 g l(-1)) was achieved in the 3/4 Murashige and Skoog (MS) medium group.
26137876	5	14	theme	root	902:905	arg1	weight					907:912	The maximum dry root weight	886:912	The maximum dry root weight (4.728 g l(-1))	886:928	The maximum dry root weight (4.728 g l(-1)) was achieved in the 3/4 Murashige and Skoog (MS) medium group.
26137876	7	15	theme	native	1344:1349	arg1	NR					1358:1359	NR	1358:1359	NR	1358:1359	The delayed-type hypersensitivity (DTH) reaction on the mice induced by 2,4-dinitrofluorobenzene (DNFB) was applied to compare the immunocompetence effects of adventitious roots (AR) with field native roots (NR) of P. heterophylla.
26137876	7	15	theme	native	1344:1349	arg1	roots					1351:1355	field native roots	1338:1355	field native roots (NR) of P. heterophylla	1338:1379	The delayed-type hypersensitivity (DTH) reaction on the mice induced by 2,4-dinitrofluorobenzene (DNFB) was applied to compare the immunocompetence effects of adventitious roots (AR) with field native roots (NR) of P. heterophylla.
26137876	3	16	theme	adventitious	502:513	arg1	roots					515:519	adventitious roots	502:519	adventitious roots	502:519	The culture conditions of adventitious roots were optimized, and datasets were subjected to a partial least squares discriminant analysis (PLS-DA), in which the growth ratio and some compounds showed a positive correlation with an aeration volume of 0.3 vvm and inoculum density of 0.15 %.
26137876	1	17	theme	ionization	187:196	arg1	ESI-MS					224:229	ESI-MS	224:229	ESI-MS(n)	224:232	A electrospray ionization tandem mass spectrometry (ESI-MS(n)) analysis was performed in order to identify the active composition in Pseudostellaria heterophylla adventitious roots.
26137876	1	17	theme	ionization	187:196	arg1	spectrometry					210:221	A electrospray ionization tandem mass spectrometry	172:221	A electrospray ionization tandem mass spectrometry (ESI-MS(n)) analysis	172:242	A electrospray ionization tandem mass spectrometry (ESI-MS(n)) analysis was performed in order to identify the active composition in Pseudostellaria heterophylla adventitious roots.
26137876	5	18	theme	medium	979:984	arg1	group					986:990	the 3/4 Murashige and Skoog (MS) medium group	946:990	the 3/4 Murashige and Skoog (MS) medium group	946:990	The maximum dry root weight (4.728 g l(-1)) was achieved in the 3/4 Murashige and Skoog (MS) medium group.
26137876	1	19	from	composition	290:300	arg1	roots					347:351	Pseudostellaria heterophylla adventitious roots	305:351	Pseudostellaria heterophylla adventitious roots	305:351	A electrospray ionization tandem mass spectrometry (ESI-MS(n)) analysis was performed in order to identify the active composition in Pseudostellaria heterophylla adventitious roots.
26137876	3	20	theme	roots	515:519	arg1	conditions					488:497	The culture conditions	476:497	The culture conditions of adventitious roots	476:519	The culture conditions of adventitious roots were optimized, and datasets were subjected to a partial least squares discriminant analysis (PLS-DA), in which the growth ratio and some compounds showed a positive correlation with an aeration volume of 0.3 vvm and inoculum density of 0.15 %.
26137876	1	21	theme	tandem	198:203	arg1	ESI-MS					224:229	ESI-MS	224:229	ESI-MS(n)	224:232	A electrospray ionization tandem mass spectrometry (ESI-MS(n)) analysis was performed in order to identify the active composition in Pseudostellaria heterophylla adventitious roots.
26137876	1	21	theme	tandem	198:203	arg1	spectrometry					210:221	A electrospray ionization tandem mass spectrometry	172:221	A electrospray ionization tandem mass spectrometry (ESI-MS(n)) analysis	172:242	A electrospray ionization tandem mass spectrometry (ESI-MS(n)) analysis was performed in order to identify the active composition in Pseudostellaria heterophylla adventitious roots.
26137876	0	22	theme	Compound	66:73	arg1	Biosynthesis					75:86	Bioactive Compound Biosynthesis	56:86	Bioactive Compound Biosynthesis	56:86	HPLC-ESI-MS(n) Analysis, Fed-Batch Cultivation Enhances Bioactive Compound Biosynthesis and Immune-Regulative Effect of Adventitious Roots in Pseudostellaria heterophylla.
26137876	6	23	theme	medium	1136:1141	arg1	group					1143:1147	the MS medium group	1129:1147	the MS medium group	1129:1147	PLS-DA showed that polysaccharides contributed significantly to the clustering of different groups and showed a positive correlation in the MS medium group.
26137876	1	24	theme	mass	205:208	arg1	ESI-MS					224:229	ESI-MS	224:229	ESI-MS(n)	224:232	A electrospray ionization tandem mass spectrometry (ESI-MS(n)) analysis was performed in order to identify the active composition in Pseudostellaria heterophylla adventitious roots.
26137876	1	24	theme	mass	205:208	arg1	spectrometry					210:221	A electrospray ionization tandem mass spectrometry	172:221	A electrospray ionization tandem mass spectrometry (ESI-MS(n)) analysis	172:242	A electrospray ionization tandem mass spectrometry (ESI-MS(n)) analysis was performed in order to identify the active composition in Pseudostellaria heterophylla adventitious roots.
26137876	3	25	theme	aeration	707:714	arg1	volume					716:721	an aeration volume	704:721	an aeration volume of 0.3 vvm	704:732	The culture conditions of adventitious roots were optimized, and datasets were subjected to a partial least squares discriminant analysis (PLS-DA), in which the growth ratio and some compounds showed a positive correlation with an aeration volume of 0.3 vvm and inoculum density of 0.15 %.
26137876	6	26	theme	MS	1133:1134	arg1	group					1143:1147	the MS medium group	1129:1147	the MS medium group	1129:1147	PLS-DA showed that polysaccharides contributed significantly to the clustering of different groups and showed a positive correlation in the MS medium group.
26137876	1	27	theme	spectrometry	210:221	arg1	analysis					235:242	A electrospray ionization tandem mass spectrometry (ESI-MS(n)) analysis	172:242	A electrospray ionization tandem mass spectrometry (ESI-MS(n)) analysis	172:242	A electrospray ionization tandem mass spectrometry (ESI-MS(n)) analysis was performed in order to identify the active composition in Pseudostellaria heterophylla adventitious roots.
26137876	0	28	theme	Roots	133:137	arg1	Biosynthesis					75:86	Bioactive Compound Biosynthesis	56:86	Bioactive Compound Biosynthesis	56:86	HPLC-ESI-MS(n) Analysis, Fed-Batch Cultivation Enhances Bioactive Compound Biosynthesis and Immune-Regulative Effect of Adventitious Roots in Pseudostellaria heterophylla.
26137876	0	28	theme	Roots	133:137	arg1	Effect					110:115	Immune-Regulative Effect	92:115	Immune-Regulative Effect of Adventitious Roots in Pseudostellaria heterophylla	92:169	HPLC-ESI-MS(n) Analysis, Fed-Batch Cultivation Enhances Bioactive Compound Biosynthesis and Immune-Regulative Effect of Adventitious Roots in Pseudostellaria heterophylla.
26137876	2	29	dep	P.	406:407	arg1	heterophylla					409:420	heterophylla	409:420	heterophylla	409:420	Pseudostellarin A, C, D, and G were identified from P. heterophylla adventitious roots on the basis of LC-MS(n) analysis.
26137876	2	30	theme	adventitious	422:433	arg1	roots					435:439	P. heterophylla adventitious roots	406:439	P. heterophylla adventitious roots	406:439	Pseudostellarin A, C, D, and G were identified from P. heterophylla adventitious roots on the basis of LC-MS(n) analysis.
26137876	1	31	theme	active	283:288	arg1	composition					290:300	the active composition	279:300	the active composition in Pseudostellaria heterophylla adventitious roots	279:351	A electrospray ionization tandem mass spectrometry (ESI-MS(n)) analysis was performed in order to identify the active composition in Pseudostellaria heterophylla adventitious roots.
26137876	0	32	theme	Adventitious	120:131	arg1	Roots					133:137	Adventitious Roots	120:137	Adventitious Roots	120:137	HPLC-ESI-MS(n) Analysis, Fed-Batch Cultivation Enhances Bioactive Compound Biosynthesis and Immune-Regulative Effect of Adventitious Roots in Pseudostellaria heterophylla.
26137876	0	33	from	Biosynthesis	75:86	arg1	heterophylla					158:169	heterophylla	158:169	heterophylla	158:169	HPLC-ESI-MS(n) Analysis, Fed-Batch Cultivation Enhances Bioactive Compound Biosynthesis and Immune-Regulative Effect of Adventitious Roots in Pseudostellaria heterophylla.
26137876	3	34	theme	culture	480:486	arg1	conditions					488:497	The culture conditions	476:497	The culture conditions of adventitious roots	476:519	The culture conditions of adventitious roots were optimized, and datasets were subjected to a partial least squares discriminant analysis (PLS-DA), in which the growth ratio and some compounds showed a positive correlation with an aeration volume of 0.3 vvm and inoculum density of 0.15 %.
26137876	8	35	theme	similar	1410:1416	arg1	function					1435:1442	a similar immunoregulation function	1408:1442	a similar immunoregulation function	1408:1442	As a result, AR possessed a similar immunoregulation function as NR.
26137876	8	35	theme	similar	1410:1416	arg1	result					1387:1392	a result	1385:1392	a result	1385:1392	As a result, AR possessed a similar immunoregulation function as NR.
26137876	2	36	theme	P.	406:407	arg1	roots					435:439	P. heterophylla adventitious roots	406:439	P. heterophylla adventitious roots	406:439	Pseudostellarin A, C, D, and G were identified from P. heterophylla adventitious roots on the basis of LC-MS(n) analysis.
26137876	0	37	dep	Cultivation	35:45	arg1	Fed-Batch					25:33	HPLC-ESI-MS(n) Analysis, Fed-Batch Cultivation	0:45	Fed-Batch	25:33	HPLC-ESI-MS(n) Analysis, Fed-Batch Cultivation Enhances Bioactive Compound Biosynthesis and Immune-Regulative Effect of Adventitious Roots in Pseudostellaria heterophylla.
26137876	6	38	theme	groups	1085:1090	arg1	clustering					1061:1070	the clustering	1057:1070	the clustering of different groups	1057:1090	PLS-DA showed that polysaccharides contributed significantly to the clustering of different groups and showed a positive correlation in the MS medium group.
26137876	0	39	theme	Analysis	15:22	arg1	Cultivation					35:45	HPLC-ESI-MS(n) Analysis, Fed-Batch Cultivation	0:45	Cultivation	35:45	HPLC-ESI-MS(n) Analysis, Fed-Batch Cultivation Enhances Bioactive Compound Biosynthesis and Immune-Regulative Effect of Adventitious Roots in Pseudostellaria heterophylla.
26137876	3	40	theme	vvm	730:732	arg1	volume					716:721	an aeration volume	704:721	an aeration volume of 0.3 vvm	704:732	The culture conditions of adventitious roots were optimized, and datasets were subjected to a partial least squares discriminant analysis (PLS-DA), in which the growth ratio and some compounds showed a positive correlation with an aeration volume of 0.3 vvm and inoculum density of 0.15 %.
26137876	3	40	theme	vvm	730:732	arg1	density					747:753	inoculum density	738:753	inoculum density of 0.15 %	738:763	The culture conditions of adventitious roots were optimized, and datasets were subjected to a partial least squares discriminant analysis (PLS-DA), in which the growth ratio and some compounds showed a positive correlation with an aeration volume of 0.3 vvm and inoculum density of 0.15 %.
26137876	2	41	theme	LC-MS	457:461	arg1	analysis					466:473	LC-MS(n) analysis	457:473	LC-MS(n) analysis	457:473	Pseudostellarin A, C, D, and G were identified from P. heterophylla adventitious roots on the basis of LC-MS(n) analysis.
26137876	5	42	theme	Skoog	968:972	arg1	group					986:990	the 3/4 Murashige and Skoog (MS) medium group	946:990	the 3/4 Murashige and Skoog (MS) medium group	946:990	The maximum dry root weight (4.728 g l(-1)) was achieved in the 3/4 Murashige and Skoog (MS) medium group.
26137876	3	43	theme	partial	570:576	arg1	squares					584:590	a partial least squares	568:590	a partial least squares discriminant analysis (PLS-DA)	568:621	The culture conditions of adventitious roots were optimized, and datasets were subjected to a partial least squares discriminant analysis (PLS-DA), in which the growth ratio and some compounds showed a positive correlation with an aeration volume of 0.3 vvm and inoculum density of 0.15 %.
26137876	1	44	theme	Pseudostellaria	305:319	arg1	roots					347:351	Pseudostellaria heterophylla adventitious roots	305:351	Pseudostellaria heterophylla adventitious roots	305:351	A electrospray ionization tandem mass spectrometry (ESI-MS(n)) analysis was performed in order to identify the active composition in Pseudostellaria heterophylla adventitious roots.
26137876	4	45	theme	Fed-batch	766:774	arg1	cultivation					776:786	Fed-batch cultivation	766:786	Fed-batch cultivation	766:786	Fed-batch cultivation enhanced the contents of total saponin, polysaccharides, and specific oxygen uptaker rate (SOUR).
26137876	7	46	theme	hypersensitivity	1167:1182	arg1	reaction					1190:1197	The delayed-type hypersensitivity (DTH) reaction	1150:1197	The delayed-type hypersensitivity (DTH) reaction on the mice induced by 2,4-dinitrofluorobenzene (DNFB)	1150:1252	The delayed-type hypersensitivity (DTH) reaction on the mice induced by 2,4-dinitrofluorobenzene (DNFB) was applied to compare the immunocompetence effects of adventitious roots (AR) with field native roots (NR) of P. heterophylla.
26137876	1	47	theme	heterophylla	321:332	arg1	roots					347:351	Pseudostellaria heterophylla adventitious roots	305:351	Pseudostellaria heterophylla adventitious roots	305:351	A electrospray ionization tandem mass spectrometry (ESI-MS(n)) analysis was performed in order to identify the active composition in Pseudostellaria heterophylla adventitious roots.
26137876	4	48	theme	rate	873:876	arg1	contents					801:808	the contents	797:808	the contents of total saponin, polysaccharides, and specific oxygen uptaker rate (SOUR)	797:883	Fed-batch cultivation enhanced the contents of total saponin, polysaccharides, and specific oxygen uptaker rate (SOUR).
26137876	7	49	theme	heterophylla	1368:1379	arg1	NR					1358:1359	NR	1358:1359	NR	1358:1359	The delayed-type hypersensitivity (DTH) reaction on the mice induced by 2,4-dinitrofluorobenzene (DNFB) was applied to compare the immunocompetence effects of adventitious roots (AR) with field native roots (NR) of P. heterophylla.
26137876	7	49	theme	heterophylla	1368:1379	arg1	roots					1351:1355	field native roots	1338:1355	field native roots (NR) of P. heterophylla	1338:1379	The delayed-type hypersensitivity (DTH) reaction on the mice induced by 2,4-dinitrofluorobenzene (DNFB) was applied to compare the immunocompetence effects of adventitious roots (AR) with field native roots (NR) of P. heterophylla.
26137876	0	50	dep	Analysis	15:22	arg1	n					12:12	n	12:12	n	12:12	HPLC-ESI-MS(n) Analysis, Fed-Batch Cultivation Enhances Bioactive Compound Biosynthesis and Immune-Regulative Effect of Adventitious Roots in Pseudostellaria heterophylla.
26137876	0	50	dep	Analysis	15:22	arg1	HPLC-ESI-MS					0:10	HPLC-ESI-MS	0:10	HPLC-ESI-MS	0:10	HPLC-ESI-MS(n) Analysis, Fed-Batch Cultivation Enhances Bioactive Compound Biosynthesis and Immune-Regulative Effect of Adventitious Roots in Pseudostellaria heterophylla.
26137876	7	51	from	reaction	1190:1197	arg1	mice					1206:1209	the mice	1202:1209	the mice induced by 2,4-dinitrofluorobenzene (DNFB)	1202:1252	The delayed-type hypersensitivity (DTH) reaction on the mice induced by 2,4-dinitrofluorobenzene (DNFB) was applied to compare the immunocompetence effects of adventitious roots (AR) with field native roots (NR) of P. heterophylla.
26137876	2	52	theme	Pseudostellarin	354:368	arg1	A					370:370	Pseudostellarin A	354:370	Pseudostellarin A	354:370	Pseudostellarin A, C, D, and G were identified from P. heterophylla adventitious roots on the basis of LC-MS(n) analysis.
26137876	6	53	theme	positive	1105:1112	arg1	correlation					1114:1124	a positive correlation	1103:1124	a positive correlation	1103:1124	PLS-DA showed that polysaccharides contributed significantly to the clustering of different groups and showed a positive correlation in the MS medium group.
26137876	1	54	theme	adventitious	334:345	arg1	roots					347:351	Pseudostellaria heterophylla adventitious roots	305:351	Pseudostellaria heterophylla adventitious roots	305:351	A electrospray ionization tandem mass spectrometry (ESI-MS(n)) analysis was performed in order to identify the active composition in Pseudostellaria heterophylla adventitious roots.
26137876	4	55	theme	oxygen	858:863	arg1	rate					873:876	specific oxygen uptaker rate	849:876	specific oxygen uptaker rate (SOUR)	849:883	Fed-batch cultivation enhanced the contents of total saponin, polysaccharides, and specific oxygen uptaker rate (SOUR).
26137876	4	55	theme	oxygen	858:863	arg1	SOUR					879:882	SOUR	879:882	SOUR	879:882	Fed-batch cultivation enhanced the contents of total saponin, polysaccharides, and specific oxygen uptaker rate (SOUR).
26137876	0	56	from	Effect	110:115	arg1	heterophylla					158:169	heterophylla	158:169	heterophylla	158:169	HPLC-ESI-MS(n) Analysis, Fed-Batch Cultivation Enhances Bioactive Compound Biosynthesis and Immune-Regulative Effect of Adventitious Roots in Pseudostellaria heterophylla.
26137876	3	57	theme	discriminant	592:603	arg1	PLS-DA					615:620	PLS-DA	615:620	PLS-DA	615:620	The culture conditions of adventitious roots were optimized, and datasets were subjected to a partial least squares discriminant analysis (PLS-DA), in which the growth ratio and some compounds showed a positive correlation with an aeration volume of 0.3 vvm and inoculum density of 0.15 %.
26137876	3	57	theme	discriminant	592:603	arg1	analysis					605:612	a partial least squares discriminant analysis	568:612	a partial least squares discriminant analysis (PLS-DA)	568:621	The culture conditions of adventitious roots were optimized, and datasets were subjected to a partial least squares discriminant analysis (PLS-DA), in which the growth ratio and some compounds showed a positive correlation with an aeration volume of 0.3 vvm and inoculum density of 0.15 %.
26137876	7	58	theme	adventitious	1309:1320	arg1	roots					1322:1326	adventitious roots	1309:1326	adventitious roots (AR)	1309:1331	The delayed-type hypersensitivity (DTH) reaction on the mice induced by 2,4-dinitrofluorobenzene (DNFB) was applied to compare the immunocompetence effects of adventitious roots (AR) with field native roots (NR) of P. heterophylla.
26137876	7	58	theme	adventitious	1309:1320	arg1	AR					1329:1330	AR	1329:1330	AR	1329:1330	The delayed-type hypersensitivity (DTH) reaction on the mice induced by 2,4-dinitrofluorobenzene (DNFB) was applied to compare the immunocompetence effects of adventitious roots (AR) with field native roots (NR) of P. heterophylla.
26137876	7	59	theme	immunocompetence	1281:1296	arg1	effects					1298:1304	the immunocompetence effects	1277:1304	the immunocompetence effects of adventitious roots (AR)	1277:1331	The delayed-type hypersensitivity (DTH) reaction on the mice induced by 2,4-dinitrofluorobenzene (DNFB) was applied to compare the immunocompetence effects of adventitious roots (AR) with field native roots (NR) of P. heterophylla.
26137876	4	60	theme	specific	849:856	arg1	rate					873:876	specific oxygen uptaker rate	849:876	specific oxygen uptaker rate (SOUR)	849:883	Fed-batch cultivation enhanced the contents of total saponin, polysaccharides, and specific oxygen uptaker rate (SOUR).
26137876	4	60	theme	specific	849:856	arg1	SOUR					879:882	SOUR	879:882	SOUR	879:882	Fed-batch cultivation enhanced the contents of total saponin, polysaccharides, and specific oxygen uptaker rate (SOUR).
26137876	5	61	theme	g	921:921	arg1	l					923:923	4.728 g l(-1)	915:927	4.728 g l(-1)	915:927	The maximum dry root weight (4.728 g l(-1)) was achieved in the 3/4 Murashige and Skoog (MS) medium group.
26137876	5	61	theme	g	921:921	arg1	weight					907:912	The maximum dry root weight	886:912	The maximum dry root weight (4.728 g l(-1))	886:928	The maximum dry root weight (4.728 g l(-1)) was achieved in the 3/4 Murashige and Skoog (MS) medium group.
26137876	7	62	theme	delayed-type	1154:1165	arg1	DTH					1185:1187	DTH	1185:1187	DTH	1185:1187	The delayed-type hypersensitivity (DTH) reaction on the mice induced by 2,4-dinitrofluorobenzene (DNFB) was applied to compare the immunocompetence effects of adventitious roots (AR) with field native roots (NR) of P. heterophylla.
26137876	7	62	theme	delayed-type	1154:1165	arg1	hypersensitivity					1167:1182	delayed-type hypersensitivity	1154:1182	The delayed-type hypersensitivity (DTH) reaction on the mice induced by 2,4-dinitrofluorobenzene (DNFB)	1150:1252	The delayed-type hypersensitivity (DTH) reaction on the mice induced by 2,4-dinitrofluorobenzene (DNFB) was applied to compare the immunocompetence effects of adventitious roots (AR) with field native roots (NR) of P. heterophylla.
26137876	4	63	theme	uptaker	865:871	arg1	rate					873:876	specific oxygen uptaker rate	849:876	specific oxygen uptaker rate (SOUR)	849:883	Fed-batch cultivation enhanced the contents of total saponin, polysaccharides, and specific oxygen uptaker rate (SOUR).
26137876	4	63	theme	uptaker	865:871	arg1	SOUR					879:882	SOUR	879:882	SOUR	879:882	Fed-batch cultivation enhanced the contents of total saponin, polysaccharides, and specific oxygen uptaker rate (SOUR).
26137876	0	64	theme	Bioactive	56:64	arg1	Biosynthesis					75:86	Bioactive Compound Biosynthesis	56:86	Bioactive Compound Biosynthesis	56:86	HPLC-ESI-MS(n) Analysis, Fed-Batch Cultivation Enhances Bioactive Compound Biosynthesis and Immune-Regulative Effect of Adventitious Roots in Pseudostellaria heterophylla.
26137876	3	65	theme	least	578:582	arg1	squares					584:590	a partial least squares	568:590	a partial least squares discriminant analysis (PLS-DA)	568:621	The culture conditions of adventitious roots were optimized, and datasets were subjected to a partial least squares discriminant analysis (PLS-DA), in which the growth ratio and some compounds showed a positive correlation with an aeration volume of 0.3 vvm and inoculum density of 0.15 %.
26137876	5	66	theme	maximum	890:896	arg1	l					923:923	4.728 g l(-1)	915:927	4.728 g l(-1)	915:927	The maximum dry root weight (4.728 g l(-1)) was achieved in the 3/4 Murashige and Skoog (MS) medium group.
26137876	5	66	theme	maximum	890:896	arg1	weight					907:912	The maximum dry root weight	886:912	The maximum dry root weight (4.728 g l(-1))	886:928	The maximum dry root weight (4.728 g l(-1)) was achieved in the 3/4 Murashige and Skoog (MS) medium group.
26137876	3	67	with	correlation	687:697	arg1	volume					716:721	an aeration volume	704:721	an aeration volume of 0.3 vvm	704:732	The culture conditions of adventitious roots were optimized, and datasets were subjected to a partial least squares discriminant analysis (PLS-DA), in which the growth ratio and some compounds showed a positive correlation with an aeration volume of 0.3 vvm and inoculum density of 0.15 %.
26137876	3	67	with	correlation	687:697	arg1	density					747:753	inoculum density	738:753	inoculum density of 0.15 %	738:763	The culture conditions of adventitious roots were optimized, and datasets were subjected to a partial least squares discriminant analysis (PLS-DA), in which the growth ratio and some compounds showed a positive correlation with an aeration volume of 0.3 vvm and inoculum density of 0.15 %.
26137876	3	68	theme	inoculum	738:745	arg1	density					747:753	inoculum density	738:753	inoculum density of 0.15 %	738:763	The culture conditions of adventitious roots were optimized, and datasets were subjected to a partial least squares discriminant analysis (PLS-DA), in which the growth ratio and some compounds showed a positive correlation with an aeration volume of 0.3 vvm and inoculum density of 0.15 %.
26137876	2	69	theme	analysis	466:473	arg1	basis					448:452	the basis	444:452	the basis of LC-MS(n) analysis	444:473	Pseudostellarin A, C, D, and G were identified from P. heterophylla adventitious roots on the basis of LC-MS(n) analysis.
26137876	3	70	theme	squares	584:590	arg1	PLS-DA					615:620	PLS-DA	615:620	PLS-DA	615:620	The culture conditions of adventitious roots were optimized, and datasets were subjected to a partial least squares discriminant analysis (PLS-DA), in which the growth ratio and some compounds showed a positive correlation with an aeration volume of 0.3 vvm and inoculum density of 0.15 %.
26137876	3	70	theme	squares	584:590	arg1	analysis					605:612	a partial least squares discriminant analysis	568:612	a partial least squares discriminant analysis (PLS-DA)	568:621	The culture conditions of adventitious roots were optimized, and datasets were subjected to a partial least squares discriminant analysis (PLS-DA), in which the growth ratio and some compounds showed a positive correlation with an aeration volume of 0.3 vvm and inoculum density of 0.15 %.
26137876	6	71	theme	different	1075:1083	arg1	groups					1085:1090	different groups	1075:1090	different groups	1075:1090	PLS-DA showed that polysaccharides contributed significantly to the clustering of different groups and showed a positive correlation in the MS medium group.
26137876	8	72	theme	immunoregulation	1418:1433	arg1	function					1435:1442	a similar immunoregulation function	1408:1442	a similar immunoregulation function	1408:1442	As a result, AR possessed a similar immunoregulation function as NR.
26137876	8	72	theme	immunoregulation	1418:1433	arg1	result					1387:1392	a result	1385:1392	a result	1385:1392	As a result, AR possessed a similar immunoregulation function as NR.
26256186	0	0	theme	Cr	107:108	arg1	adsorption					93:102	adsorption	93:102	adsorption of Cr(VI)	93:112	Microwave preparation of triethylenetetramine modified graphene oxide/chitosan composite for adsorption of Cr(VI).
26256186	3	1	from	Adsorption	397:406	arg1	composite					425:433	the composite	421:433	the composite	421:433	Adsorption of Cr(VI) on the composite was studied.
26256186	6	2	theme	219.5mg	796:802	arg1	-1					806:807	-1	806:807	-1	806:807	The highest adsorption capacity of 219.5mg g(-1) was obtained at pH 2.
26256186	6	2	theme	219.5mg	796:802	arg1	g					804:804	219.5mg g	796:804	219.5mg g(-1)	796:808	The highest adsorption capacity of 219.5mg g(-1) was obtained at pH 2.
26256186	7	3	theme	kinetic	872:878	arg1	model					880:884	pseudo-second-order kinetic model	852:884	pseudo-second-order kinetic model	852:884	Adsorption followed pseudo-second-order kinetic model and Langmuir isotherm.
26256186	10	4	dep	Cr	1138:1139	arg1	removal					1145:1151	removal	1145:1151	Cr(VI) removal in effluents	1138:1164	These results have important implications for the application expansion of microwave preparation and the design of new effective composites for Cr(VI) removal in effluents.
26256186	10	4	dep	Cr	1138:1139	arg1	VI					1141:1142	VI	1141:1142	VI	1141:1142	These results have important implications for the application expansion of microwave preparation and the design of new effective composites for Cr(VI) removal in effluents.
26256186	7	5	theme	Langmuir	890:897	arg1	isotherm					899:906	Langmuir isotherm	890:906	Langmuir isotherm	890:906	Adsorption followed pseudo-second-order kinetic model and Langmuir isotherm.
26256186	4	6	contain	had	515:517	arg2	uptake					536:541	uptake	536:541	uptake	536:541	The experimental results indicated that the product obtained by MW had higher yield and uptake than one obtained by the conventional and uptake of TGOCS for Cr(VI) was higher than that of the recently reported adsorbents.
26256186	4	6	contain	had	515:517	arg2	yield					526:530	higher yield	519:530	higher yield	519:530	The experimental results indicated that the product obtained by MW had higher yield and uptake than one obtained by the conventional and uptake of TGOCS for Cr(VI) was higher than that of the recently reported adsorbents.
26256186	4	6	contain	had	515:517	arg1	product					492:498	the product	488:498	the product obtained by MW	488:513	The experimental results indicated that the product obtained by MW had higher yield and uptake than one obtained by the conventional and uptake of TGOCS for Cr(VI) was higher than that of the recently reported adsorbents.
26256186	4	7	theme	reported	649:656	arg1	adsorbents					658:667	the recently reported adsorbents	636:667	the recently reported adsorbents	636:667	The experimental results indicated that the product obtained by MW had higher yield and uptake than one obtained by the conventional and uptake of TGOCS for Cr(VI) was higher than that of the recently reported adsorbents.
26256186	10	8	theme	important	1013:1021	arg1	implications					1023:1034	important implications	1013:1034	important implications for the application expansion of microwave preparation	1013:1089	These results have important implications for the application expansion of microwave preparation and the design of new effective composites for Cr(VI) removal in effluents.
26256186	7	9	theme	pseudo-second-order	852:870	arg1	model					880:884	pseudo-second-order kinetic model	852:884	pseudo-second-order kinetic model	852:884	Adsorption followed pseudo-second-order kinetic model and Langmuir isotherm.
26256186	5	10	dep	Cr	720:721	arg1	VI					723:724	VI	723:724	VI	723:724	The effects of various variables on adsorption of Cr(VI) by TGOCS were further researched.
26256186	6	11	theme	g	804:804	arg1	capacity					784:791	The highest adsorption capacity	761:791	The highest adsorption capacity of 219.5mg g(-1)	761:808	The highest adsorption capacity of 219.5mg g(-1) was obtained at pH 2.
26256186	4	12	dep	higher	616:621	arg1	VI					608:609	VI	608:609	VI	608:609	The experimental results indicated that the product obtained by MW had higher yield and uptake than one obtained by the conventional and uptake of TGOCS for Cr(VI) was higher than that of the recently reported adsorbents.
26256186	2	13	theme	elemental	377:385	arg1	analysis					387:394	elemental analysis	377:394	elemental analysis	377:394	This composite was characterized by FTIR, XRD, SEM, BET and elemental analysis.
26256186	4	14	theme	experimental	452:463	arg1	results					465:471	The experimental results	448:471	The experimental results	448:471	The experimental results indicated that the product obtained by MW had higher yield and uptake than one obtained by the conventional and uptake of TGOCS for Cr(VI) was higher than that of the recently reported adsorbents.
26256186	0	15	dep	Cr	107:108	arg1	VI					110:111	VI	110:111	VI	110:111	Microwave preparation of triethylenetetramine modified graphene oxide/chitosan composite for adsorption of Cr(VI).
26256186	10	16	contain	have	1008:1011	arg1	results					1000:1006	These results	994:1006	These results	994:1006	These results have important implications for the application expansion of microwave preparation and the design of new effective composites for Cr(VI) removal in effluents.
26256186	10	16	contain	have	1008:1011	arg2	design					1099:1104	the design	1095:1104	the design of new effective composites for Cr(VI) removal in effluents	1095:1164	These results have important implications for the application expansion of microwave preparation and the design of new effective composites for Cr(VI) removal in effluents.
26256186	10	16	contain	have	1008:1011	arg2	implications					1023:1034	important implications	1013:1034	important implications for the application expansion of microwave preparation	1013:1089	These results have important implications for the application expansion of microwave preparation and the design of new effective composites for Cr(VI) removal in effluents.
26256186	0	17	theme	Microwave	0:8	arg1	preparation					10:20	Microwave preparation	0:20	Microwave preparation of triethylenetetramine	0:44	Microwave preparation of triethylenetetramine modified graphene oxide/chitosan composite for adsorption of Cr(VI).
26256186	10	18	theme	microwave	1069:1077	arg1	preparation					1079:1089	microwave preparation	1069:1089	microwave preparation	1069:1089	These results have important implications for the application expansion of microwave preparation and the design of new effective composites for Cr(VI) removal in effluents.
26256186	3	19	dep	Cr	411:412	arg1	VI					414:415	VI	414:415	VI	414:415	Adsorption of Cr(VI) on the composite was studied.
26256186	0	20	theme	triethylenetetramine	25:44	arg1	preparation					10:20	Microwave preparation	0:20	Microwave preparation of triethylenetetramine	0:44	Microwave preparation of triethylenetetramine modified graphene oxide/chitosan composite for adsorption of Cr(VI).
26256186	4	21	theme	higher	519:524	arg1	yield					526:530	higher yield	519:530	higher yield	519:530	The experimental results indicated that the product obtained by MW had higher yield and uptake than one obtained by the conventional and uptake of TGOCS for Cr(VI) was higher than that of the recently reported adsorbents.
26256186	4	22	theme	TGOCS	595:599	arg1	uptake					585:590	uptake	585:590	uptake of TGOCS for Cr	585:606	The experimental results indicated that the product obtained by MW had higher yield and uptake than one obtained by the conventional and uptake of TGOCS for Cr(VI) was higher than that of the recently reported adsorbents.
26256186	1	23	theme	conventional	295:306	arg1	heating					308:314	conventional heating	295:314	conventional heating	295:314	A novel triethylenetetramine modified graphene oxide/chitosan composite (TGOCS) was successfully synthesized by microwave irradiation (MW) method and compared with one prepared by conventional heating.
26256186	5	24	from	effects	674:680	arg1	adsorption					706:715	adsorption	706:715	adsorption of Cr(VI) by TGOCS	706:734	The effects of various variables on adsorption of Cr(VI) by TGOCS were further researched.
26256186	0	25	theme	graphene	55:62	arg1	composite					79:87	graphene oxide/chitosan composite	55:87	graphene oxide/chitosan composite for adsorption of Cr(VI)	55:112	Microwave preparation of triethylenetetramine modified graphene oxide/chitosan composite for adsorption of Cr(VI).
26256186	10	26	theme	application	1044:1054	arg1	expansion					1056:1064	the application expansion	1040:1064	the application expansion of microwave preparation	1040:1089	These results have important implications for the application expansion of microwave preparation and the design of new effective composites for Cr(VI) removal in effluents.
26256186	1	27	theme	microwave	227:235	arg1	MW					250:251	MW	250:251	MW	250:251	A novel triethylenetetramine modified graphene oxide/chitosan composite (TGOCS) was successfully synthesized by microwave irradiation (MW) method and compared with one prepared by conventional heating.
26256186	1	27	theme	microwave	227:235	arg1	irradiation					237:247	microwave irradiation	227:247	microwave irradiation (MW) method	227:259	A novel triethylenetetramine modified graphene oxide/chitosan composite (TGOCS) was successfully synthesized by microwave irradiation (MW) method and compared with one prepared by conventional heating.
26256186	3	28	theme	Cr	411:412	arg1	Adsorption					397:406	Adsorption	397:406	Adsorption of Cr(VI) on the composite	397:433	Adsorption of Cr(VI) on the composite was studied.
26256186	5	29	theme	Cr	720:721	arg1	adsorption					706:715	adsorption	706:715	adsorption of Cr(VI) by TGOCS	706:734	The effects of various variables on adsorption of Cr(VI) by TGOCS were further researched.
26256186	6	30	theme	adsorption	773:782	arg1	capacity					784:791	The highest adsorption capacity	761:791	The highest adsorption capacity of 219.5mg g(-1)	761:808	The highest adsorption capacity of 219.5mg g(-1) was obtained at pH 2.
26256186	1	31	theme	irradiation	237:247	arg1	method					254:259	microwave irradiation (MW) method	227:259	microwave irradiation (MW) method	227:259	A novel triethylenetetramine modified graphene oxide/chitosan composite (TGOCS) was successfully synthesized by microwave irradiation (MW) method and compared with one prepared by conventional heating.
26256186	10	32	from	Cr	1138:1139	arg1	effluents					1156:1164	effluents	1156:1164	effluents	1156:1164	These results have important implications for the application expansion of microwave preparation and the design of new effective composites for Cr(VI) removal in effluents.
26256186	6	33	theme	highest	765:771	arg1	capacity					784:791	The highest adsorption capacity	761:791	The highest adsorption capacity of 219.5mg g(-1)	761:808	The highest adsorption capacity of 219.5mg g(-1) was obtained at pH 2.
26256186	0	34	theme	oxide/chitosan	64:77	arg1	composite					79:87	graphene oxide/chitosan composite	55:87	graphene oxide/chitosan composite for adsorption of Cr(VI)	55:112	Microwave preparation of triethylenetetramine modified graphene oxide/chitosan composite for adsorption of Cr(VI).
26256186	1	35	theme	novel	117:121	arg1	TGOCS					188:192	TGOCS	188:192	TGOCS	188:192	A novel triethylenetetramine modified graphene oxide/chitosan composite (TGOCS) was successfully synthesized by microwave irradiation (MW) method and compared with one prepared by conventional heating.
26256186	1	35	theme	novel	117:121	arg1	composite					177:185	A novel triethylenetetramine modified graphene oxide/chitosan composite	115:185	A novel triethylenetetramine modified graphene oxide/chitosan composite (TGOCS)	115:193	A novel triethylenetetramine modified graphene oxide/chitosan composite (TGOCS) was successfully synthesized by microwave irradiation (MW) method and compared with one prepared by conventional heating.
26256186	10	36	theme	preparation	1079:1089	arg1	expansion					1056:1064	the application expansion	1040:1064	the application expansion of microwave preparation	1040:1089	These results have important implications for the application expansion of microwave preparation and the design of new effective composites for Cr(VI) removal in effluents.
26256186	1	37	theme	triethylenetetramine	123:142	arg1	TGOCS					188:192	TGOCS	188:192	TGOCS	188:192	A novel triethylenetetramine modified graphene oxide/chitosan composite (TGOCS) was successfully synthesized by microwave irradiation (MW) method and compared with one prepared by conventional heating.
26256186	1	37	theme	triethylenetetramine	123:142	arg1	composite					177:185	A novel triethylenetetramine modified graphene oxide/chitosan composite	115:185	A novel triethylenetetramine modified graphene oxide/chitosan composite (TGOCS)	115:193	A novel triethylenetetramine modified graphene oxide/chitosan composite (TGOCS) was successfully synthesized by microwave irradiation (MW) method and compared with one prepared by conventional heating.
26256186	10	38	theme	composites	1123:1132	arg1	implications					1023:1034	important implications	1013:1034	important implications for the application expansion of microwave preparation	1013:1089	These results have important implications for the application expansion of microwave preparation and the design of new effective composites for Cr(VI) removal in effluents.
26256186	10	38	theme	composites	1123:1132	arg1	design					1099:1104	the design	1095:1104	the design of new effective composites for Cr(VI) removal in effluents	1095:1164	These results have important implications for the application expansion of microwave preparation and the design of new effective composites for Cr(VI) removal in effluents.
26256186	1	39	theme	modified	144:151	arg1	TGOCS					188:192	TGOCS	188:192	TGOCS	188:192	A novel triethylenetetramine modified graphene oxide/chitosan composite (TGOCS) was successfully synthesized by microwave irradiation (MW) method and compared with one prepared by conventional heating.
26256186	1	39	theme	modified	144:151	arg1	composite					177:185	A novel triethylenetetramine modified graphene oxide/chitosan composite	115:185	A novel triethylenetetramine modified graphene oxide/chitosan composite (TGOCS)	115:193	A novel triethylenetetramine modified graphene oxide/chitosan composite (TGOCS) was successfully synthesized by microwave irradiation (MW) method and compared with one prepared by conventional heating.
26256186	10	40	theme	effective	1113:1121	arg1	composites					1123:1132	new effective composites	1109:1132	new effective composites for Cr(VI) removal in effluents	1109:1164	These results have important implications for the application expansion of microwave preparation and the design of new effective composites for Cr(VI) removal in effluents.
26256186	5	41	theme	various	685:691	arg1	variables					693:701	various variables	685:701	various variables	685:701	The effects of various variables on adsorption of Cr(VI) by TGOCS were further researched.
26256186	1	42	theme	graphene	153:160	arg1	TGOCS					188:192	TGOCS	188:192	TGOCS	188:192	A novel triethylenetetramine modified graphene oxide/chitosan composite (TGOCS) was successfully synthesized by microwave irradiation (MW) method and compared with one prepared by conventional heating.
26256186	1	42	theme	graphene	153:160	arg1	composite					177:185	A novel triethylenetetramine modified graphene oxide/chitosan composite	115:185	A novel triethylenetetramine modified graphene oxide/chitosan composite (TGOCS)	115:193	A novel triethylenetetramine modified graphene oxide/chitosan composite (TGOCS) was successfully synthesized by microwave irradiation (MW) method and compared with one prepared by conventional heating.
26256186	10	43	theme	new	1109:1111	arg1	composites					1123:1132	new effective composites	1109:1132	new effective composites for Cr(VI) removal in effluents	1109:1164	These results have important implications for the application expansion of microwave preparation and the design of new effective composites for Cr(VI) removal in effluents.
26256186	5	44	theme	variables	693:701	arg1	effects					674:680	The effects	670:680	The effects of various variables on adsorption of Cr(VI) by TGOCS	670:734	The effects of various variables on adsorption of Cr(VI) by TGOCS were further researched.
26256186	1	45	theme	oxide/chitosan	162:175	arg1	TGOCS					188:192	TGOCS	188:192	TGOCS	188:192	A novel triethylenetetramine modified graphene oxide/chitosan composite (TGOCS) was successfully synthesized by microwave irradiation (MW) method and compared with one prepared by conventional heating.
26256186	1	45	theme	oxide/chitosan	162:175	arg1	composite					177:185	A novel triethylenetetramine modified graphene oxide/chitosan composite	115:185	A novel triethylenetetramine modified graphene oxide/chitosan composite (TGOCS)	115:193	A novel triethylenetetramine modified graphene oxide/chitosan composite (TGOCS) was successfully synthesized by microwave irradiation (MW) method and compared with one prepared by conventional heating.
27561468	1	0	from	ZDY2013	202:208	arg1	EPS					157:159	EPS	157:159	EPS	157:159	An exopolysaccharide (EPS) from probiotics Lactobacillus plantarum ZDY2013 was purified to illustrate its molecular weight, monosaccharide composition and biological activities.
27561468	1	0	from	ZDY2013	202:208	arg1	exopolysaccharide					138:154	An exopolysaccharide	135:154	An exopolysaccharide (EPS) from probiotics Lactobacillus plantarum ZDY2013	135:208	An exopolysaccharide (EPS) from probiotics Lactobacillus plantarum ZDY2013 was purified to illustrate its molecular weight, monosaccharide composition and biological activities.
27561468	1	1	theme	probiotics	167:176	arg1	ZDY2013					202:208	probiotics Lactobacillus plantarum ZDY2013	167:208	probiotics Lactobacillus plantarum ZDY2013	167:208	An exopolysaccharide (EPS) from probiotics Lactobacillus plantarum ZDY2013 was purified to illustrate its molecular weight, monosaccharide composition and biological activities.
27561468	7	2	from	effective	1010:1018	arg1	addition					978:985	addition	978:985	addition	978:985	In addition, sulfated EPS was more effective in counteracting the cytotoxicity induced by B. cereus enterotoxins on Caco-2 cells when compared with EPS.
27561468	7	3	theme	Caco-2	1091:1096	arg1	cells					1098:1102	Caco-2 cells	1091:1102	Caco-2 cells	1091:1102	In addition, sulfated EPS was more effective in counteracting the cytotoxicity induced by B. cereus enterotoxins on Caco-2 cells when compared with EPS.
27561468	3	4	contain	have	414:417	arg1	EPS					389:391	The EPS	385:391	The EPS	385:391	The EPS was characterized to have only one symmetrical sharp peak by high-performance size-exclusion chromatography and its molecular weight was 5.17×10(4)Da.
27561468	3	4	contain	have	414:417	arg2	peak					446:449	only one symmetrical sharp peak	419:449	only one symmetrical sharp peak	419:449	The EPS was characterized to have only one symmetrical sharp peak by high-performance size-exclusion chromatography and its molecular weight was 5.17×10(4)Da.
27561468	1	5	theme	Lactobacillus	178:190	arg1	ZDY2013					202:208	probiotics Lactobacillus plantarum ZDY2013	167:208	probiotics Lactobacillus plantarum ZDY2013	167:208	An exopolysaccharide (EPS) from probiotics Lactobacillus plantarum ZDY2013 was purified to illustrate its molecular weight, monosaccharide composition and biological activities.
27561468	1	6	theme	biological	290:299	arg1	activities					301:310	biological activities	290:310	biological activities	290:310	An exopolysaccharide (EPS) from probiotics Lactobacillus plantarum ZDY2013 was purified to illustrate its molecular weight, monosaccharide composition and biological activities.
27561468	0	7	theme	biological	112:121	arg1	activities					123:132	its biological activities	108:132	its biological activities	108:132	Characterization and sulfated modification of an exopolysaccharide from Lactobacillus plantarum ZDY2013 and its biological activities.
27561468	0	8	from	Characterization	0:15	arg1	ZDY2013					96:102	Lactobacillus plantarum ZDY2013	72:102	Lactobacillus plantarum ZDY2013	72:102	Characterization and sulfated modification of an exopolysaccharide from Lactobacillus plantarum ZDY2013 and its biological activities.
27561468	0	8	from	Characterization	0:15	arg1	activities					123:132	its biological activities	108:132	its biological activities	108:132	Characterization and sulfated modification of an exopolysaccharide from Lactobacillus plantarum ZDY2013 and its biological activities.
27561468	2	9	theme	EPS	326:328	arg1	yield					317:321	The yield	313:321	The yield of EPS (429.4±30.3mg/L)	313:345	The yield of EPS (429.4±30.3mg/L) was obtained with a purity of 96.06%.
27561468	6	10	dep	EPS	873:875	arg1	Both					851:854	Both	851:854	Both	851:854	Both EPS and sulfated EPS showed radical scavenging activities, and the antioxidant activities increased after sulfonation.
27561468	6	10	dep	EPS	873:875	arg1	sulfated					864:871	sulfated	864:871	sulfated	864:871	Both EPS and sulfated EPS showed radical scavenging activities, and the antioxidant activities increased after sulfonation.
27561468	4	11	theme	monosaccharides	682:696	arg1	monosaccharides					682:696	the total monosaccharides	672:696	the total monosaccharides	672:696	The GC analysis revealed that EPS only consisted of xylose and galactose, and the galactose possessed as high as 98.3% (w/w) of the total monosaccharides.
27561468	4	11	theme	monosaccharides	682:696	arg1	w/w					664:666	w/w	664:666	w/w	664:666	The GC analysis revealed that EPS only consisted of xylose and galactose, and the galactose possessed as high as 98.3% (w/w) of the total monosaccharides.
27561468	4	11	theme	monosaccharides	682:696	arg1	%					661:661	98.3%	657:661	98.3% (w/w) of the total monosaccharides	657:696	The GC analysis revealed that EPS only consisted of xylose and galactose, and the galactose possessed as high as 98.3% (w/w) of the total monosaccharides.
27561468	8	12	from	ZDY203	1240:1245	arg1	activities					1204:1213	the biological activities	1189:1213	the biological activities of EPS from L. plantarum ZDY203	1189:1245	In summary, sulfonation is a feasible strategy for improving the biological activities of EPS from L. plantarum ZDY203.
27561468	8	12	from	ZDY203	1240:1245	arg1	EPS					1218:1220	EPS	1218:1220	EPS from L. plantarum ZDY203	1218:1245	In summary, sulfonation is a feasible strategy for improving the biological activities of EPS from L. plantarum ZDY203.
27561468	1	13	theme	plantarum	192:200	arg1	ZDY2013					202:208	probiotics Lactobacillus plantarum ZDY2013	167:208	probiotics Lactobacillus plantarum ZDY2013	167:208	An exopolysaccharide (EPS) from probiotics Lactobacillus plantarum ZDY2013 was purified to illustrate its molecular weight, monosaccharide composition and biological activities.
27561468	7	14	dep	B.	1065:1066	arg1	cereus					1068:1073	cereus	1068:1073	cereus	1068:1073	In addition, sulfated EPS was more effective in counteracting the cytotoxicity induced by B. cereus enterotoxins on Caco-2 cells when compared with EPS.
27561468	8	15	from	strategy	1166:1173	arg1	summary					1131:1137	summary	1131:1137	summary	1131:1137	In summary, sulfonation is a feasible strategy for improving the biological activities of EPS from L. plantarum ZDY203.
27561468	7	16	theme	B.	1065:1066	arg1	enterotoxins					1075:1086	B. cereus enterotoxins	1065:1086	B. cereus enterotoxins on Caco-2 cells	1065:1102	In addition, sulfated EPS was more effective in counteracting the cytotoxicity induced by B. cereus enterotoxins on Caco-2 cells when compared with EPS.
27561468	6	17	theme	radical	884:890	arg1	activities					903:912	radical scavenging activities	884:912	radical scavenging activities	884:912	Both EPS and sulfated EPS showed radical scavenging activities, and the antioxidant activities increased after sulfonation.
27561468	5	18	theme	sulfated	717:724	arg1	EPS					726:728	a sulfated EPS	715:728	a sulfated EPS	715:728	By sulfonation, a sulfated EPS was successfully synthesized with the degree of substitution (DS) of 0.29, which was confirmed using FT-IR spectroscopy.
27561468	3	19	theme	symmetrical	428:438	arg1	peak					446:449	only one symmetrical sharp peak	419:449	only one symmetrical sharp peak	419:449	The EPS was characterized to have only one symmetrical sharp peak by high-performance size-exclusion chromatography and its molecular weight was 5.17×10(4)Da.
27561468	5	20	theme	substitution	778:789	arg1	degree					768:773	the degree	764:773	the degree of substitution (DS) of 0.29, which was confirmed using FT-IR spectroscopy	764:848	By sulfonation, a sulfated EPS was successfully synthesized with the degree of substitution (DS) of 0.29, which was confirmed using FT-IR spectroscopy.
27561468	6	21	theme	antioxidant	923:933	arg1	activities					935:944	the antioxidant activities	919:944	the antioxidant activities	919:944	Both EPS and sulfated EPS showed radical scavenging activities, and the antioxidant activities increased after sulfonation.
27561468	8	22	theme	biological	1193:1202	arg1	activities					1204:1213	the biological activities	1189:1213	the biological activities of EPS from L. plantarum ZDY203	1189:1245	In summary, sulfonation is a feasible strategy for improving the biological activities of EPS from L. plantarum ZDY203.
27561468	3	23	theme	sharp	440:444	arg1	peak					446:449	only one symmetrical sharp peak	419:449	only one symmetrical sharp peak	419:449	The EPS was characterized to have only one symmetrical sharp peak by high-performance size-exclusion chromatography and its molecular weight was 5.17×10(4)Da.
27561468	5	24	theme	0.29	799:802	arg1	substitution					778:789	substitution	778:789	substitution (DS)	778:794	By sulfonation, a sulfated EPS was successfully synthesized with the degree of substitution (DS) of 0.29, which was confirmed using FT-IR spectroscopy.
27561468	5	24	theme	0.29	799:802	arg1	DS					792:793	DS	792:793	DS	792:793	By sulfonation, a sulfated EPS was successfully synthesized with the degree of substitution (DS) of 0.29, which was confirmed using FT-IR spectroscopy.
27561468	0	25	theme	sulfated	21:28	arg1	modification					30:41	sulfated modification	21:41	sulfated modification	21:41	Characterization and sulfated modification of an exopolysaccharide from Lactobacillus plantarum ZDY2013 and its biological activities.
27561468	8	26	theme	EPS	1218:1220	arg1	activities					1204:1213	the biological activities	1189:1213	the biological activities of EPS from L. plantarum ZDY203	1189:1245	In summary, sulfonation is a feasible strategy for improving the biological activities of EPS from L. plantarum ZDY203.
27561468	0	27	from	ZDY2013	96:102	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and sulfated modification of an exopolysaccharide from Lactobacillus plantarum ZDY2013 and its biological activities.
27561468	0	27	from	ZDY2013	96:102	arg1	modification					30:41	sulfated modification	21:41	sulfated modification	21:41	Characterization and sulfated modification of an exopolysaccharide from Lactobacillus plantarum ZDY2013 and its biological activities.
27561468	0	27	from	ZDY2013	96:102	arg1	exopolysaccharide					49:65	an exopolysaccharide	46:65	an exopolysaccharide from Lactobacillus plantarum ZDY2013 and its biological activities	46:132	Characterization and sulfated modification of an exopolysaccharide from Lactobacillus plantarum ZDY2013 and its biological activities.
27561468	3	28	theme	high-performance	454:469	arg1	chromatography					486:499	high-performance size-exclusion chromatography	454:499	high-performance size-exclusion chromatography	454:499	The EPS was characterized to have only one symmetrical sharp peak by high-performance size-exclusion chromatography and its molecular weight was 5.17×10(4)Da.
27561468	8	29	theme	feasible	1157:1164	arg1	strategy					1166:1173	a feasible strategy	1155:1173	a feasible strategy for improving the biological activities of EPS from L. plantarum ZDY203	1155:1245	In summary, sulfonation is a feasible strategy for improving the biological activities of EPS from L. plantarum ZDY203.
27561468	8	29	theme	feasible	1157:1164	arg1	sulfonation					1140:1150	sulfonation	1140:1150	sulfonation	1140:1150	In summary, sulfonation is a feasible strategy for improving the biological activities of EPS from L. plantarum ZDY203.
27561468	8	30	theme	plantarum	1230:1238	arg1	ZDY203					1240:1245	L. plantarum ZDY203	1227:1245	L. plantarum ZDY203	1227:1245	In summary, sulfonation is a feasible strategy for improving the biological activities of EPS from L. plantarum ZDY203.
27561468	6	31	dep	Both	851:854	arg1	EPS					856:858	EPS	856:858	EPS	856:858	Both EPS and sulfated EPS showed radical scavenging activities, and the antioxidant activities increased after sulfonation.
27561468	3	32	theme	size-exclusion	471:484	arg1	chromatography					486:499	high-performance size-exclusion chromatography	454:499	high-performance size-exclusion chromatography	454:499	The EPS was characterized to have only one symmetrical sharp peak by high-performance size-exclusion chromatography and its molecular weight was 5.17×10(4)Da.
27561468	6	33	theme	scavenging	892:901	arg1	activities					903:912	radical scavenging activities	884:912	radical scavenging activities	884:912	Both EPS and sulfated EPS showed radical scavenging activities, and the antioxidant activities increased after sulfonation.
27561468	6	34	dep	showed	877:882	arg1	increased					946:954	increased	946:954	showed radical scavenging activities, and the antioxidant activities increased after sulfonation	877:972	Both EPS and sulfated EPS showed radical scavenging activities, and the antioxidant activities increased after sulfonation.
27561468	8	35	from	activities	1204:1213	arg1	ZDY203					1240:1245	L. plantarum ZDY203	1227:1245	L. plantarum ZDY203	1227:1245	In summary, sulfonation is a feasible strategy for improving the biological activities of EPS from L. plantarum ZDY203.
27561468	4	36	theme	total	676:680	arg1	monosaccharides					682:696	the total monosaccharides	672:696	the total monosaccharides	672:696	The GC analysis revealed that EPS only consisted of xylose and galactose, and the galactose possessed as high as 98.3% (w/w) of the total monosaccharides.
27561468	0	37	theme	exopolysaccharide	49:65	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and sulfated modification of an exopolysaccharide from Lactobacillus plantarum ZDY2013 and its biological activities.
27561468	0	37	theme	exopolysaccharide	49:65	arg1	modification					30:41	sulfated modification	21:41	sulfated modification	21:41	Characterization and sulfated modification of an exopolysaccharide from Lactobacillus plantarum ZDY2013 and its biological activities.
27561468	4	38	theme	GC	548:549	arg1	analysis					551:558	The GC analysis	544:558	The GC analysis	544:558	The GC analysis revealed that EPS only consisted of xylose and galactose, and the galactose possessed as high as 98.3% (w/w) of the total monosaccharides.
27561468	7	39	from	addition	978:985	arg1	effective					1010:1018	effective	1010:1018	effective	1010:1018	In addition, sulfated EPS was more effective in counteracting the cytotoxicity induced by B. cereus enterotoxins on Caco-2 cells when compared with EPS.
27561468	7	40	theme	sulfated	988:995	arg1	EPS					997:999	sulfated EPS	988:999	sulfated EPS	988:999	In addition, sulfated EPS was more effective in counteracting the cytotoxicity induced by B. cereus enterotoxins on Caco-2 cells when compared with EPS.
27561468	0	41	from	activities	123:132	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and sulfated modification of an exopolysaccharide from Lactobacillus plantarum ZDY2013 and its biological activities.
27561468	0	41	from	activities	123:132	arg1	modification					30:41	sulfated modification	21:41	sulfated modification	21:41	Characterization and sulfated modification of an exopolysaccharide from Lactobacillus plantarum ZDY2013 and its biological activities.
27561468	0	41	from	activities	123:132	arg1	exopolysaccharide					49:65	an exopolysaccharide	46:65	an exopolysaccharide from Lactobacillus plantarum ZDY2013 and its biological activities	46:132	Characterization and sulfated modification of an exopolysaccharide from Lactobacillus plantarum ZDY2013 and its biological activities.
27561468	8	42	theme	L.	1227:1228	arg1	ZDY203					1240:1245	L. plantarum ZDY203	1227:1245	L. plantarum ZDY203	1227:1245	In summary, sulfonation is a feasible strategy for improving the biological activities of EPS from L. plantarum ZDY203.
27561468	3	43	theme	molecular	509:517	arg1	weight					519:524	its molecular weight	505:524	its molecular weight	505:524	The EPS was characterized to have only one symmetrical sharp peak by high-performance size-exclusion chromatography and its molecular weight was 5.17×10(4)Da.
27561468	0	44	theme	plantarum	86:94	arg1	ZDY2013					96:102	Lactobacillus plantarum ZDY2013	72:102	Lactobacillus plantarum ZDY2013	72:102	Characterization and sulfated modification of an exopolysaccharide from Lactobacillus plantarum ZDY2013 and its biological activities.
27561468	1	45	theme	molecular	241:249	arg1	weight					251:256	its molecular weight	237:256	its molecular weight	237:256	An exopolysaccharide (EPS) from probiotics Lactobacillus plantarum ZDY2013 was purified to illustrate its molecular weight, monosaccharide composition and biological activities.
27561468	5	46	theme	FT-IR	831:835	arg1	spectroscopy					837:848	FT-IR spectroscopy	831:848	FT-IR spectroscopy	831:848	By sulfonation, a sulfated EPS was successfully synthesized with the degree of substitution (DS) of 0.29, which was confirmed using FT-IR spectroscopy.
27561468	1	47	theme	monosaccharide	259:272	arg1	composition					274:284	monosaccharide composition	259:284	monosaccharide composition	259:284	An exopolysaccharide (EPS) from probiotics Lactobacillus plantarum ZDY2013 was purified to illustrate its molecular weight, monosaccharide composition and biological activities.
27561468	0	48	theme	Lactobacillus	72:84	arg1	ZDY2013					96:102	Lactobacillus plantarum ZDY2013	72:102	Lactobacillus plantarum ZDY2013	72:102	Characterization and sulfated modification of an exopolysaccharide from Lactobacillus plantarum ZDY2013 and its biological activities.
27561468	0	49	from	modification	30:41	arg1	ZDY2013					96:102	Lactobacillus plantarum ZDY2013	72:102	Lactobacillus plantarum ZDY2013	72:102	Characterization and sulfated modification of an exopolysaccharide from Lactobacillus plantarum ZDY2013 and its biological activities.
27561468	0	49	from	modification	30:41	arg1	activities					123:132	its biological activities	108:132	its biological activities	108:132	Characterization and sulfated modification of an exopolysaccharide from Lactobacillus plantarum ZDY2013 and its biological activities.
27561468	2	50	theme	%	382:382	arg1	purity					367:372	a purity	365:372	a purity of 96.06%	365:382	The yield of EPS (429.4±30.3mg/L) was obtained with a purity of 96.06%.
27561468	7	51	from	enterotoxins	1075:1086	arg1	cells					1098:1102	Caco-2 cells	1091:1102	Caco-2 cells	1091:1102	In addition, sulfated EPS was more effective in counteracting the cytotoxicity induced by B. cereus enterotoxins on Caco-2 cells when compared with EPS.
26616962	0	0	theme	EPS	76:78	arg1	production					43:52	production	43:52	production	43:52	The influence of fermentation condition on production and molecular mass of EPS produced by Streptococcus thermophilus 05-34 in milk-based medium.
26616962	0	0	theme	EPS	76:78	arg1	mass					68:71	molecular mass	58:71	molecular mass	58:71	The influence of fermentation condition on production and molecular mass of EPS produced by Streptococcus thermophilus 05-34 in milk-based medium.
26616962	3	1	theme	fermentation	658:669	arg1	condition					671:679	the non-optimal fermentation condition	642:679	the non-optimal fermentation condition	642:679	This EPS was with a molecular mass of 4.7×10(5) Da, which was increased by 9 times compared with that in the non-optimal fermentation condition, while monosaccharide composition did not change.
26616962	0	2	from	influence	4:12	arg1	production					43:52	production	43:52	production	43:52	The influence of fermentation condition on production and molecular mass of EPS produced by Streptococcus thermophilus 05-34 in milk-based medium.
26616962	0	2	from	influence	4:12	arg1	mass					68:71	molecular mass	58:71	molecular mass	58:71	The influence of fermentation condition on production and molecular mass of EPS produced by Streptococcus thermophilus 05-34 in milk-based medium.
26616962	2	3	theme	1.0:0.8	528:534	arg1	ratio					519:523	a molar ratio	511:523	a molar ratio of 1.0:0.8	511:534	Structure analysis showed that EPS produced under the optimal fermentation condition was composed of galactose and glucose in a molar ratio of 1.0:0.8.
26616962	1	4	dep	4.2-fold	304:311	arg1	condition					283:291	the optimal fermentation condition	258:291	the optimal fermentation condition	258:291	The yield of exopolysaccharide (EPS) produced by Streptococcus thermophilus 05-34 reached up to 250 mg/L under the optimal fermentation condition, which was 4.2-fold higher than that produced under the non-optimal fermentation condition.
26616962	1	4	dep	4.2-fold	304:311	arg1	4.2-fold					304:311	4.2-fold	304:311	4.2-fold	304:311	The yield of exopolysaccharide (EPS) produced by Streptococcus thermophilus 05-34 reached up to 250 mg/L under the optimal fermentation condition, which was 4.2-fold higher than that produced under the non-optimal fermentation condition.
26616962	1	4	dep	4.2-fold	304:311	arg1	higher					313:318	higher	313:318	higher	313:318	The yield of exopolysaccharide (EPS) produced by Streptococcus thermophilus 05-34 reached up to 250 mg/L under the optimal fermentation condition, which was 4.2-fold higher than that produced under the non-optimal fermentation condition.
26616962	4	5	theme	chain-length	841:852	arg1	determination					854:866	chain-length determination	841:866	chain-length determination	841:866	Furthermore, real-time quantitative PCR showed that the transcription level of epsC, which is responsible for chain-length determination, was up-regulated by 2.7-fold, suggesting that the increased molecular mass of EPS was resulted from improving polymerization degree of monosaccharide.
26616962	3	6	theme	monosaccharide	688:701	arg1	composition					703:713	monosaccharide composition	688:713	monosaccharide composition	688:713	This EPS was with a molecular mass of 4.7×10(5) Da, which was increased by 9 times compared with that in the non-optimal fermentation condition, while monosaccharide composition did not change.
26616962	4	7	theme	real-time	744:752	arg1	PCR					767:769	real-time quantitative PCR	744:769	real-time quantitative PCR	744:769	Furthermore, real-time quantitative PCR showed that the transcription level of epsC, which is responsible for chain-length determination, was up-regulated by 2.7-fold, suggesting that the increased molecular mass of EPS was resulted from improving polymerization degree of monosaccharide.
26616962	1	8	theme	exopolysaccharide	160:176	arg1	yield					151:155	The yield	147:155	The yield of exopolysaccharide (EPS) produced by Streptococcus thermophilus 05-34	147:227	The yield of exopolysaccharide (EPS) produced by Streptococcus thermophilus 05-34 reached up to 250 mg/L under the optimal fermentation condition, which was 4.2-fold higher than that produced under the non-optimal fermentation condition.
26616962	0	9	theme	Streptococcus	92:104	arg1	thermophilus					106:117	Streptococcus thermophilus 05-34	92:123	Streptococcus thermophilus 05-34	92:123	The influence of fermentation condition on production and molecular mass of EPS produced by Streptococcus thermophilus 05-34 in milk-based medium.
26616962	1	10	theme	fermentation	361:372	arg1	condition					374:382	the non-optimal fermentation condition	345:382	the non-optimal fermentation condition	345:382	The yield of exopolysaccharide (EPS) produced by Streptococcus thermophilus 05-34 reached up to 250 mg/L under the optimal fermentation condition, which was 4.2-fold higher than that produced under the non-optimal fermentation condition.
26616962	4	11	theme	epsC	810:813	arg1	level					801:805	the transcription level	783:805	the transcription level of epsC, which is responsible for chain-length determination,	783:867	Furthermore, real-time quantitative PCR showed that the transcription level of epsC, which is responsible for chain-length determination, was up-regulated by 2.7-fold, suggesting that the increased molecular mass of EPS was resulted from improving polymerization degree of monosaccharide.
26616962	5	12	theme	EPS	1102:1104	arg1	mass					1116:1119	EPS molecular mass	1102:1119	EPS molecular mass	1102:1119	These findings demonstrated that the optimized fermentation condition can improve EPS molecular mass, and may consequently modify the rheological properties of EPS.
26616962	4	13	theme	monosaccharide	1004:1017	arg1	degree					994:999	polymerization degree	979:999	polymerization degree of monosaccharide	979:1017	Furthermore, real-time quantitative PCR showed that the transcription level of epsC, which is responsible for chain-length determination, was up-regulated by 2.7-fold, suggesting that the increased molecular mass of EPS was resulted from improving polymerization degree of monosaccharide.
26616962	3	14	theme	molecular	557:565	arg1	mass					567:570	a molecular mass	555:570	a molecular mass of 4.7×10(5) Da, which was increased by 9 times compared with that in the non-optimal fermentation condition	555:679	This EPS was with a molecular mass of 4.7×10(5) Da, which was increased by 9 times compared with that in the non-optimal fermentation condition, while monosaccharide composition did not change.
26616962	1	15	theme	non-optimal	349:359	arg1	condition					374:382	the non-optimal fermentation condition	345:382	the non-optimal fermentation condition	345:382	The yield of exopolysaccharide (EPS) produced by Streptococcus thermophilus 05-34 reached up to 250 mg/L under the optimal fermentation condition, which was 4.2-fold higher than that produced under the non-optimal fermentation condition.
26616962	2	16	theme	Structure	385:393	arg1	analysis					395:402	Structure analysis	385:402	Structure analysis	385:402	Structure analysis showed that EPS produced under the optimal fermentation condition was composed of galactose and glucose in a molar ratio of 1.0:0.8.
26616962	2	17	theme	fermentation	447:458	arg1	condition					460:468	the optimal fermentation condition	435:468	the optimal fermentation condition	435:468	Structure analysis showed that EPS produced under the optimal fermentation condition was composed of galactose and glucose in a molar ratio of 1.0:0.8.
26616962	1	18	theme	optimal	262:268	arg1	condition					283:291	the optimal fermentation condition	258:291	the optimal fermentation condition	258:291	The yield of exopolysaccharide (EPS) produced by Streptococcus thermophilus 05-34 reached up to 250 mg/L under the optimal fermentation condition, which was 4.2-fold higher than that produced under the non-optimal fermentation condition.
26616962	1	18	theme	optimal	262:268	arg1	4.2-fold					304:311	4.2-fold	304:311	4.2-fold	304:311	The yield of exopolysaccharide (EPS) produced by Streptococcus thermophilus 05-34 reached up to 250 mg/L under the optimal fermentation condition, which was 4.2-fold higher than that produced under the non-optimal fermentation condition.
26616962	1	18	theme	optimal	262:268	arg1	higher					313:318	higher	313:318	higher	313:318	The yield of exopolysaccharide (EPS) produced by Streptococcus thermophilus 05-34 reached up to 250 mg/L under the optimal fermentation condition, which was 4.2-fold higher than that produced under the non-optimal fermentation condition.
26616962	0	19	theme	fermentation	17:28	arg1	condition					30:38	fermentation condition	17:38	fermentation condition	17:38	The influence of fermentation condition on production and molecular mass of EPS produced by Streptococcus thermophilus 05-34 in milk-based medium.
26616962	1	20	theme	fermentation	270:281	arg1	condition					283:291	the optimal fermentation condition	258:291	the optimal fermentation condition	258:291	The yield of exopolysaccharide (EPS) produced by Streptococcus thermophilus 05-34 reached up to 250 mg/L under the optimal fermentation condition, which was 4.2-fold higher than that produced under the non-optimal fermentation condition.
26616962	1	20	theme	fermentation	270:281	arg1	4.2-fold					304:311	4.2-fold	304:311	4.2-fold	304:311	The yield of exopolysaccharide (EPS) produced by Streptococcus thermophilus 05-34 reached up to 250 mg/L under the optimal fermentation condition, which was 4.2-fold higher than that produced under the non-optimal fermentation condition.
26616962	1	20	theme	fermentation	270:281	arg1	higher					313:318	higher	313:318	higher	313:318	The yield of exopolysaccharide (EPS) produced by Streptococcus thermophilus 05-34 reached up to 250 mg/L under the optimal fermentation condition, which was 4.2-fold higher than that produced under the non-optimal fermentation condition.
26616962	5	21	theme	optimized	1057:1065	arg1	condition					1080:1088	the optimized fermentation condition	1053:1088	the optimized fermentation condition	1053:1088	These findings demonstrated that the optimized fermentation condition can improve EPS molecular mass, and may consequently modify the rheological properties of EPS.
26616962	5	22	theme	fermentation	1067:1078	arg1	condition					1080:1088	the optimized fermentation condition	1053:1088	the optimized fermentation condition	1053:1088	These findings demonstrated that the optimized fermentation condition can improve EPS molecular mass, and may consequently modify the rheological properties of EPS.
26616962	4	23	theme	transcription	787:799	arg1	level					801:805	the transcription level	783:805	the transcription level of epsC, which is responsible for chain-length determination,	783:867	Furthermore, real-time quantitative PCR showed that the transcription level of epsC, which is responsible for chain-length determination, was up-regulated by 2.7-fold, suggesting that the increased molecular mass of EPS was resulted from improving polymerization degree of monosaccharide.
26616962	1	24	theme	Streptococcus	196:208	arg1	thermophilus					210:221	Streptococcus thermophilus 05-34	196:227	Streptococcus thermophilus 05-34	196:227	The yield of exopolysaccharide (EPS) produced by Streptococcus thermophilus 05-34 reached up to 250 mg/L under the optimal fermentation condition, which was 4.2-fold higher than that produced under the non-optimal fermentation condition.
26616962	0	25	theme	condition	30:38	arg1	influence					4:12	The influence	0:12	The influence of fermentation condition on production and molecular mass of EPS produced by Streptococcus thermophilus 05-34 in milk-based medium.	0:145	The influence of fermentation condition on production and molecular mass of EPS produced by Streptococcus thermophilus 05-34 in milk-based medium.
26616962	0	26	theme	milk-based	128:137	arg1	medium					139:144	milk-based medium	128:144	milk-based medium	128:144	The influence of fermentation condition on production and molecular mass of EPS produced by Streptococcus thermophilus 05-34 in milk-based medium.
26616962	2	27	theme	optimal	439:445	arg1	condition					460:468	the optimal fermentation condition	435:468	the optimal fermentation condition	435:468	Structure analysis showed that EPS produced under the optimal fermentation condition was composed of galactose and glucose in a molar ratio of 1.0:0.8.
26616962	2	28	theme	molar	513:517	arg1	ratio					519:523	a molar ratio	511:523	a molar ratio of 1.0:0.8	511:534	Structure analysis showed that EPS produced under the optimal fermentation condition was composed of galactose and glucose in a molar ratio of 1.0:0.8.
26616962	4	29	theme	increased	919:927	arg1	mass					939:942	the increased molecular mass	915:942	the increased molecular mass of EPS	915:949	Furthermore, real-time quantitative PCR showed that the transcription level of epsC, which is responsible for chain-length determination, was up-regulated by 2.7-fold, suggesting that the increased molecular mass of EPS was resulted from improving polymerization degree of monosaccharide.
26616962	5	30	theme	EPS	1180:1182	arg1	properties					1166:1175	the rheological properties	1150:1175	the rheological properties of EPS	1150:1182	These findings demonstrated that the optimized fermentation condition can improve EPS molecular mass, and may consequently modify the rheological properties of EPS.
26616962	5	31	theme	molecular	1106:1114	arg1	mass					1116:1119	EPS molecular mass	1102:1119	EPS molecular mass	1102:1119	These findings demonstrated that the optimized fermentation condition can improve EPS molecular mass, and may consequently modify the rheological properties of EPS.
26616962	4	32	theme	polymerization	979:992	arg1	degree					994:999	polymerization degree	979:999	polymerization degree of monosaccharide	979:1017	Furthermore, real-time quantitative PCR showed that the transcription level of epsC, which is responsible for chain-length determination, was up-regulated by 2.7-fold, suggesting that the increased molecular mass of EPS was resulted from improving polymerization degree of monosaccharide.
26616962	5	33	theme	rheological	1154:1164	arg1	properties					1166:1175	the rheological properties	1150:1175	the rheological properties of EPS	1150:1182	These findings demonstrated that the optimized fermentation condition can improve EPS molecular mass, and may consequently modify the rheological properties of EPS.
26616962	1	34	dep	250	243:245	arg1	to					240:241	to	240:241	to	240:241	The yield of exopolysaccharide (EPS) produced by Streptococcus thermophilus 05-34 reached up to 250 mg/L under the optimal fermentation condition, which was 4.2-fold higher than that produced under the non-optimal fermentation condition.
26616962	4	35	theme	quantitative	754:765	arg1	PCR					767:769	real-time quantitative PCR	744:769	real-time quantitative PCR	744:769	Furthermore, real-time quantitative PCR showed that the transcription level of epsC, which is responsible for chain-length determination, was up-regulated by 2.7-fold, suggesting that the increased molecular mass of EPS was resulted from improving polymerization degree of monosaccharide.
26616962	3	36	dep	increased	599:607	arg1	compared					620:627	compared	620:627	compared with that in the non-optimal fermentation condition	620:679	This EPS was with a molecular mass of 4.7×10(5) Da, which was increased by 9 times compared with that in the non-optimal fermentation condition, while monosaccharide composition did not change.
26616962	4	37	theme	EPS	947:949	arg1	mass					939:942	the increased molecular mass	915:942	the increased molecular mass of EPS	915:949	Furthermore, real-time quantitative PCR showed that the transcription level of epsC, which is responsible for chain-length determination, was up-regulated by 2.7-fold, suggesting that the increased molecular mass of EPS was resulted from improving polymerization degree of monosaccharide.
26616962	0	38	theme	molecular	58:66	arg1	mass					68:71	molecular mass	58:71	molecular mass	58:71	The influence of fermentation condition on production and molecular mass of EPS produced by Streptococcus thermophilus 05-34 in milk-based medium.
26616962	3	39	theme	non-optimal	646:656	arg1	condition					671:679	the non-optimal fermentation condition	642:679	the non-optimal fermentation condition	642:679	This EPS was with a molecular mass of 4.7×10(5) Da, which was increased by 9 times compared with that in the non-optimal fermentation condition, while monosaccharide composition did not change.
26616962	3	40	theme	Da	585:586	arg1	mass					567:570	a molecular mass	555:570	a molecular mass of 4.7×10(5) Da, which was increased by 9 times compared with that in the non-optimal fermentation condition	555:679	This EPS was with a molecular mass of 4.7×10(5) Da, which was increased by 9 times compared with that in the non-optimal fermentation condition, while monosaccharide composition did not change.
26616962	4	41	theme	molecular	929:937	arg1	mass					939:942	the increased molecular mass	915:942	the increased molecular mass of EPS	915:949	Furthermore, real-time quantitative PCR showed that the transcription level of epsC, which is responsible for chain-length determination, was up-regulated by 2.7-fold, suggesting that the increased molecular mass of EPS was resulted from improving polymerization degree of monosaccharide.
24057866	1	0	theme	infertile	237:245	arg1	patients					300:307	infertile leukocytospermic and normal, fertile normozoospermic patients	237:307	infertile leukocytospermic and normal, fertile normozoospermic patients	237:307	Our study compares the status of human seminal plasma immunoglobulin G (IgG) and IgA secretory component (SC) fucosylation between infertile leukocytospermic and normal, fertile normozoospermic patients.
24057866	5	1	attach	present	793:799	arg1	forms					808:812	two forms	804:812	two forms: 78-kDa and 63-kDa	804:831	In seminal plasma the SC is present in two forms: 78-kDa and 63-kDa.
24057866	5	1	attach	present	793:799	arg2	SC					787:788	the SC	783:788	the SC	783:788	In seminal plasma the SC is present in two forms: 78-kDa and 63-kDa.
24057866	5	1	attach	present	793:799	arg1	plasma					776:781	seminal plasma	768:781	seminal plasma	768:781	In seminal plasma the SC is present in two forms: 78-kDa and 63-kDa.
24057866	5	1	attach	present	793:799	arg2	present					793:799	present	793:799	present	793:799	In seminal plasma the SC is present in two forms: 78-kDa and 63-kDa.
24057866	4	2	theme	present	593:599	arg1	IgG					589:591	The IgG	585:591	The IgG present in leukocytospermic samples	585:627	The IgG present in leukocytospermic samples is characterized by lower expression of core fucose than in the normal group (0.82 ± 0.3 AU and 1.2 ± 0.3 AU, respectively; p < 0.002).
24057866	4	3	from	present	593:599	arg1	samples					621:627	leukocytospermic samples	604:627	leukocytospermic samples	604:627	The IgG present in leukocytospermic samples is characterized by lower expression of core fucose than in the normal group (0.82 ± 0.3 AU and 1.2 ± 0.3 AU, respectively; p < 0.002).
24057866	1	4	theme	immunoglobulin	160:173	arg1	G					175:175	immunoglobulin G	160:175	immunoglobulin G (IgG)	160:181	Our study compares the status of human seminal plasma immunoglobulin G (IgG) and IgA secretory component (SC) fucosylation between infertile leukocytospermic and normal, fertile normozoospermic patients.
24057866	1	4	theme	immunoglobulin	160:173	arg1	IgG					178:180	IgG	178:180	IgG	178:180	Our study compares the status of human seminal plasma immunoglobulin G (IgG) and IgA secretory component (SC) fucosylation between infertile leukocytospermic and normal, fertile normozoospermic patients.
24057866	1	5	theme	leukocytospermic	247:262	arg1	patients					300:307	infertile leukocytospermic and normal, fertile normozoospermic patients	237:307	infertile leukocytospermic and normal, fertile normozoospermic patients	237:307	Our study compares the status of human seminal plasma immunoglobulin G (IgG) and IgA secretory component (SC) fucosylation between infertile leukocytospermic and normal, fertile normozoospermic patients.
24057866	4	6	theme	leukocytospermic	604:619	arg1	samples					621:627	leukocytospermic samples	604:627	leukocytospermic samples	604:627	The IgG present in leukocytospermic samples is characterized by lower expression of core fucose than in the normal group (0.82 ± 0.3 AU and 1.2 ± 0.3 AU, respectively; p < 0.002).
24057866	4	7	dep	AU	718:719	arg1	p < 0.002					753:761	p < 0.002	753:761	p < 0.002	753:761	The IgG present in leukocytospermic samples is characterized by lower expression of core fucose than in the normal group (0.82 ± 0.3 AU and 1.2 ± 0.3 AU, respectively; p < 0.002).
24057866	9	8	theme	IgG	1434:1436	arg1	analysis					1422:1429	The analysis	1418:1429	The analysis of IgG and SC fucosylation status	1418:1463	The analysis of IgG and SC fucosylation status and the determination of IgG and S-IgA concentrations in seminal plasma might constitute a valuable diagnosis tools for the evaluation of male infertility associated with leukocytospermia with accompanying inflammation.
24057866	9	8	theme	IgG	1434:1436	arg1	determination					1473:1485	the determination	1469:1485	the determination of IgG and S-IgA concentrations in seminal plasma	1469:1535	The analysis of IgG and SC fucosylation status and the determination of IgG and S-IgA concentrations in seminal plasma might constitute a valuable diagnosis tools for the evaluation of male infertility associated with leukocytospermia with accompanying inflammation.
24057866	3	9	theme	IgG	503:505	arg1	fucosylation					512:523	IgG core fucosylation	503:523	IgG core fucosylation	503:523	However, a correlation between IgG core fucosylation and IgG concentration (r = -0.52; p < 0.0003) was observed.
24057866	9	10	theme	SC	1442:1443	arg1	status					1458:1463	SC fucosylation status	1442:1463	SC fucosylation status	1442:1463	The analysis of IgG and SC fucosylation status and the determination of IgG and S-IgA concentrations in seminal plasma might constitute a valuable diagnosis tools for the evaluation of male infertility associated with leukocytospermia with accompanying inflammation.
24057866	8	11	dep	group	1338:1342	arg1	mg/l					1358:1361	67.68 ± 29.2 mg/l	1345:1361	67.68 ± 29.2 mg/l	1345:1361	Moreover, the present study has shown that in leukocytospermic samples the mean concentrations of IgG and S-IgA are twice as high (131.68 ± 102.6 mg/l and 36 ± 27 mg/l, respectively) as in the normal group (67.68 ± 29.2 mg/l; p < 0.02, and 19 ± 18 mg/l, p < 0.019, respectively).
24057866	8	11	dep	group	1338:1342	arg1	p < 0.019					1392:1400	p < 0.019	1392:1400	p < 0.019	1392:1400	Moreover, the present study has shown that in leukocytospermic samples the mean concentrations of IgG and S-IgA are twice as high (131.68 ± 102.6 mg/l and 36 ± 27 mg/l, respectively) as in the normal group (67.68 ± 29.2 mg/l; p < 0.02, and 19 ± 18 mg/l, p < 0.019, respectively).
24057866	5	12	theme	seminal	768:774	arg1	plasma					776:781	seminal plasma	768:781	seminal plasma	768:781	In seminal plasma the SC is present in two forms: 78-kDa and 63-kDa.
24057866	6	13	theme	present	838:844	arg1	study					846:850	The present study	834:850	The present study	834:850	The present study has also shown a higher AAL and LTA specific reactivity of glycans expressed in 63-kDa SC, in comparison to 78-kDa SC, in the normal group.
24057866	9	14	theme	status	1458:1463	arg1	analysis					1422:1429	The analysis	1418:1429	The analysis of IgG and SC fucosylation status	1418:1463	The analysis of IgG and SC fucosylation status and the determination of IgG and S-IgA concentrations in seminal plasma might constitute a valuable diagnosis tools for the evaluation of male infertility associated with leukocytospermia with accompanying inflammation.
24057866	9	14	theme	status	1458:1463	arg1	determination					1473:1485	the determination	1469:1485	the determination of IgG and S-IgA concentrations in seminal plasma	1469:1535	The analysis of IgG and SC fucosylation status and the determination of IgG and S-IgA concentrations in seminal plasma might constitute a valuable diagnosis tools for the evaluation of male infertility associated with leukocytospermia with accompanying inflammation.
24057866	3	15	theme	IgG	529:531	arg1	concentration					533:545	IgG concentration	529:545	IgG concentration (r = -0.52; p < 0.0003)	529:569	However, a correlation between IgG core fucosylation and IgG concentration (r = -0.52; p < 0.0003) was observed.
24057866	5	16	theme	78-kDa	815:820	arg1	forms					808:812	two forms	804:812	two forms: 78-kDa and 63-kDa	804:831	In seminal plasma the SC is present in two forms: 78-kDa and 63-kDa.
24057866	4	17	theme	core	669:672	arg1	fucose					674:679	core fucose	669:679	core fucose	669:679	The IgG present in leukocytospermic samples is characterized by lower expression of core fucose than in the normal group (0.82 ± 0.3 AU and 1.2 ± 0.3 AU, respectively; p < 0.002).
24057866	5	18	theme	63-kDa	826:831	arg1	forms					808:812	two forms	804:812	two forms: 78-kDa and 63-kDa	804:831	In seminal plasma the SC is present in two forms: 78-kDa and 63-kDa.
24057866	9	19	from	determination	1473:1485	arg1	plasma					1530:1535	seminal plasma	1522:1535	seminal plasma	1522:1535	The analysis of IgG and SC fucosylation status and the determination of IgG and S-IgA concentrations in seminal plasma might constitute a valuable diagnosis tools for the evaluation of male infertility associated with leukocytospermia with accompanying inflammation.
24057866	0	20	theme	secretory	49:57	arg1	component					59:67	secretory component	49:67	secretory component of IgA	49:74	Changes in fucosylation of human seminal IgG and secretory component of IgA in leukocytospermic patients.
24057866	9	21	theme	valuable	1556:1563	arg1	tools					1575:1579	a valuable diagnosis tools	1554:1579	a valuable diagnosis tools for the evaluation of male infertility associated with leukocytospermia with accompanying inflammation	1554:1682	The analysis of IgG and SC fucosylation status and the determination of IgG and S-IgA concentrations in seminal plasma might constitute a valuable diagnosis tools for the evaluation of male infertility associated with leukocytospermia with accompanying inflammation.
24057866	5	22	from	plasma	776:781	arg1	present					793:799	present	793:799	present	793:799	In seminal plasma the SC is present in two forms: 78-kDa and 63-kDa.
24057866	5	22	from	plasma	776:781	arg1	SC					787:788	the SC	783:788	the SC	783:788	In seminal plasma the SC is present in two forms: 78-kDa and 63-kDa.
24057866	6	23	theme	specific	888:895	arg1	reactivity					897:906	specific reactivity	888:906	specific reactivity	888:906	The present study has also shown a higher AAL and LTA specific reactivity of glycans expressed in 63-kDa SC, in comparison to 78-kDa SC, in the normal group.
24057866	7	24	from	leukocytospermia	995:1010	arg1	values					1017:1022	the values	1013:1022	the values	1013:1022	In leukocytospermia, the values of specific lectin reactivity for core fucose, fucose α(1-2)- and α(1-3)- linked, were similar for both SC bands.
24057866	7	24	from	leukocytospermia	995:1010	arg1	similar					1111:1117	similar	1111:1117	similar	1111:1117	In leukocytospermia, the values of specific lectin reactivity for core fucose, fucose α(1-2)- and α(1-3)- linked, were similar for both SC bands.
24057866	7	25	theme	fucose	1071:1076	arg1	1-2					1080:1082	1-2	1080:1082	1-2	1080:1082	In leukocytospermia, the values of specific lectin reactivity for core fucose, fucose α(1-2)- and α(1-3)- linked, were similar for both SC bands.
24057866	7	25	theme	fucose	1071:1076	arg1	α					1078:1078	fucose α	1071:1078	fucose α	1071:1078	In leukocytospermia, the values of specific lectin reactivity for core fucose, fucose α(1-2)- and α(1-3)- linked, were similar for both SC bands.
24057866	1	26	theme	seminal	145:151	arg1	plasma					153:158	human seminal plasma immunoglobulin G (IgG) and IgA secretory component (SC) fucosylation	139:227	plasma	153:158	Our study compares the status of human seminal plasma immunoglobulin G (IgG) and IgA secretory component (SC) fucosylation between infertile leukocytospermic and normal, fertile normozoospermic patients.
24057866	9	27	theme	concentrations	1504:1517	arg1	analysis					1422:1429	The analysis	1418:1429	The analysis of IgG and SC fucosylation status	1418:1463	The analysis of IgG and SC fucosylation status and the determination of IgG and S-IgA concentrations in seminal plasma might constitute a valuable diagnosis tools for the evaluation of male infertility associated with leukocytospermia with accompanying inflammation.
24057866	9	27	theme	concentrations	1504:1517	arg1	determination					1473:1485	the determination	1469:1485	the determination of IgG and S-IgA concentrations in seminal plasma	1469:1535	The analysis of IgG and SC fucosylation status and the determination of IgG and S-IgA concentrations in seminal plasma might constitute a valuable diagnosis tools for the evaluation of male infertility associated with leukocytospermia with accompanying inflammation.
24057866	0	28	from	Changes	0:6	arg1	fucosylation					11:22	fucosylation	11:22	fucosylation of human seminal IgG	11:43	Changes in fucosylation of human seminal IgG and secretory component of IgA in leukocytospermic patients.
24057866	0	28	from	Changes	0:6	arg1	patients					96:103	leukocytospermic patients	79:103	leukocytospermic patients	79:103	Changes in fucosylation of human seminal IgG and secretory component of IgA in leukocytospermic patients.
24057866	5	29	from	present	793:799	arg1	forms					808:812	two forms	804:812	two forms: 78-kDa and 63-kDa	804:831	In seminal plasma the SC is present in two forms: 78-kDa and 63-kDa.
24057866	5	29	from	present	793:799	arg1	plasma					776:781	seminal plasma	768:781	seminal plasma	768:781	In seminal plasma the SC is present in two forms: 78-kDa and 63-kDa.
24057866	6	30	theme	higher	869:874	arg1	AAL					876:878	a higher AAL	867:878	a higher AAL	867:878	The present study has also shown a higher AAL and LTA specific reactivity of glycans expressed in 63-kDa SC, in comparison to 78-kDa SC, in the normal group.
24057866	9	31	theme	seminal	1522:1528	arg1	plasma					1530:1535	seminal plasma	1522:1535	seminal plasma	1522:1535	The analysis of IgG and SC fucosylation status and the determination of IgG and S-IgA concentrations in seminal plasma might constitute a valuable diagnosis tools for the evaluation of male infertility associated with leukocytospermia with accompanying inflammation.
24057866	7	32	theme	SC	1128:1129	arg1	bands					1131:1135	both SC bands	1123:1135	both SC bands	1123:1135	In leukocytospermia, the values of specific lectin reactivity for core fucose, fucose α(1-2)- and α(1-3)- linked, were similar for both SC bands.
24057866	2	33	theme	Lewis	424:428	arg1	fucose					370:375	AAL-reactive core fucose	352:375	AAL-reactive core fucose	352:375	The seminal IgG and SC are decorated with AAL-reactive core fucose, and antennary UEA- and LTA-reactive fucose of Lewis(y) and Lewis(x) structures, respectively.
24057866	2	33	theme	Lewis	424:428	arg1	UEA-					392:395	antennary UEA-	382:395	antennary UEA-	382:395	The seminal IgG and SC are decorated with AAL-reactive core fucose, and antennary UEA- and LTA-reactive fucose of Lewis(y) and Lewis(x) structures, respectively.
24057866	2	33	theme	Lewis	424:428	arg1	fucose					414:419	LTA-reactive fucose	401:419	LTA-reactive fucose	401:419	The seminal IgG and SC are decorated with AAL-reactive core fucose, and antennary UEA- and LTA-reactive fucose of Lewis(y) and Lewis(x) structures, respectively.
24057866	8	34	theme	present	1152:1158	arg1	study					1160:1164	the present study	1148:1164	the present study	1148:1164	Moreover, the present study has shown that in leukocytospermic samples the mean concentrations of IgG and S-IgA are twice as high (131.68 ± 102.6 mg/l and 36 ± 27 mg/l, respectively) as in the normal group (67.68 ± 29.2 mg/l; p < 0.02, and 19 ± 18 mg/l, p < 0.019, respectively).
24057866	2	35	theme	core	365:368	arg1	fucose					370:375	AAL-reactive core fucose	352:375	AAL-reactive core fucose	352:375	The seminal IgG and SC are decorated with AAL-reactive core fucose, and antennary UEA- and LTA-reactive fucose of Lewis(y) and Lewis(x) structures, respectively.
24057866	7	36	theme	lectin	1036:1041	arg1	reactivity					1043:1052	specific lectin reactivity	1027:1052	specific lectin reactivity	1027:1052	In leukocytospermia, the values of specific lectin reactivity for core fucose, fucose α(1-2)- and α(1-3)- linked, were similar for both SC bands.
24057866	8	37	dep	high	1263:1266	arg1	mg/l					1284:1287	131.68 ± 102.6 mg/l	1269:1287	131.68 ± 102.6 mg/l	1269:1287	Moreover, the present study has shown that in leukocytospermic samples the mean concentrations of IgG and S-IgA are twice as high (131.68 ± 102.6 mg/l and 36 ± 27 mg/l, respectively) as in the normal group (67.68 ± 29.2 mg/l; p < 0.02, and 19 ± 18 mg/l, p < 0.019, respectively).
24057866	8	37	dep	high	1263:1266	arg1	mg/l					1301:1304	36 ± 27 mg/l	1293:1304	36 ± 27 mg/l	1293:1304	Moreover, the present study has shown that in leukocytospermic samples the mean concentrations of IgG and S-IgA are twice as high (131.68 ± 102.6 mg/l and 36 ± 27 mg/l, respectively) as in the normal group (67.68 ± 29.2 mg/l; p < 0.02, and 19 ± 18 mg/l, p < 0.019, respectively).
24057866	8	37	dep	high	1263:1266	arg1	high					1263:1266	high	1263:1266	high	1263:1266	Moreover, the present study has shown that in leukocytospermic samples the mean concentrations of IgG and S-IgA are twice as high (131.68 ± 102.6 mg/l and 36 ± 27 mg/l, respectively) as in the normal group (67.68 ± 29.2 mg/l; p < 0.02, and 19 ± 18 mg/l, p < 0.019, respectively).
24057866	8	37	dep	high	1263:1266	arg1	concentrations					1218:1231	the mean concentrations	1209:1231	the mean concentrations of IgG and S-IgA	1209:1248	Moreover, the present study has shown that in leukocytospermic samples the mean concentrations of IgG and S-IgA are twice as high (131.68 ± 102.6 mg/l and 36 ± 27 mg/l, respectively) as in the normal group (67.68 ± 29.2 mg/l; p < 0.02, and 19 ± 18 mg/l, p < 0.019, respectively).
24057866	1	38	theme	normal	268:273	arg1	patients					300:307	infertile leukocytospermic and normal, fertile normozoospermic patients	237:307	infertile leukocytospermic and normal, fertile normozoospermic patients	237:307	Our study compares the status of human seminal plasma immunoglobulin G (IgG) and IgA secretory component (SC) fucosylation between infertile leukocytospermic and normal, fertile normozoospermic patients.
24057866	6	39	theme	63-kDa	932:937	arg1	SC					939:940	63-kDa SC	932:940	63-kDa SC	932:940	The present study has also shown a higher AAL and LTA specific reactivity of glycans expressed in 63-kDa SC, in comparison to 78-kDa SC, in the normal group.
24057866	9	40	theme	diagnosis	1565:1573	arg1	tools					1575:1579	a valuable diagnosis tools	1554:1579	a valuable diagnosis tools for the evaluation of male infertility associated with leukocytospermia with accompanying inflammation	1554:1682	The analysis of IgG and SC fucosylation status and the determination of IgG and S-IgA concentrations in seminal plasma might constitute a valuable diagnosis tools for the evaluation of male infertility associated with leukocytospermia with accompanying inflammation.
24057866	4	41	attach	present	593:599	arg1	samples					621:627	leukocytospermic samples	604:627	leukocytospermic samples	604:627	The IgG present in leukocytospermic samples is characterized by lower expression of core fucose than in the normal group (0.82 ± 0.3 AU and 1.2 ± 0.3 AU, respectively; p < 0.002).
24057866	4	41	attach	present	593:599	arg2	IgG					589:591	The IgG	585:591	The IgG present in leukocytospermic samples	585:627	The IgG present in leukocytospermic samples is characterized by lower expression of core fucose than in the normal group (0.82 ± 0.3 AU and 1.2 ± 0.3 AU, respectively; p < 0.002).
24057866	2	42	theme	antennary	382:390	arg1	UEA-					392:395	antennary UEA-	382:395	antennary UEA-	382:395	The seminal IgG and SC are decorated with AAL-reactive core fucose, and antennary UEA- and LTA-reactive fucose of Lewis(y) and Lewis(x) structures, respectively.
24057866	0	43	theme	human	27:31	arg1	IgG					41:43	human seminal IgG	27:43	human seminal IgG	27:43	Changes in fucosylation of human seminal IgG and secretory component of IgA in leukocytospermic patients.
24057866	9	44	from	concentrations	1504:1517	arg1	plasma					1530:1535	seminal plasma	1522:1535	seminal plasma	1522:1535	The analysis of IgG and SC fucosylation status and the determination of IgG and S-IgA concentrations in seminal plasma might constitute a valuable diagnosis tools for the evaluation of male infertility associated with leukocytospermia with accompanying inflammation.
24057866	7	45	theme	linked	1098:1103	arg1	similar					1111:1117	similar	1111:1117	similar	1111:1117	In leukocytospermia, the values of specific lectin reactivity for core fucose, fucose α(1-2)- and α(1-3)- linked, were similar for both SC bands.
24057866	7	45	theme	linked	1098:1103	arg1	values					1017:1022	the values	1013:1022	the values	1013:1022	In leukocytospermia, the values of specific lectin reactivity for core fucose, fucose α(1-2)- and α(1-3)- linked, were similar for both SC bands.
24057866	8	46	theme	normal	1331:1336	arg1	group					1338:1342	the normal group	1327:1342	the normal group (67.68 ± 29.2 mg/l; p < 0.02, and 19 ± 18 mg/l, p < 0.019, respectively)	1327:1415	Moreover, the present study has shown that in leukocytospermic samples the mean concentrations of IgG and S-IgA are twice as high (131.68 ± 102.6 mg/l and 36 ± 27 mg/l, respectively) as in the normal group (67.68 ± 29.2 mg/l; p < 0.02, and 19 ± 18 mg/l, p < 0.019, respectively).
24057866	1	47	dep	plasma	153:158	arg1	G					175:175	immunoglobulin G	160:175	immunoglobulin G (IgG)	160:181	Our study compares the status of human seminal plasma immunoglobulin G (IgG) and IgA secretory component (SC) fucosylation between infertile leukocytospermic and normal, fertile normozoospermic patients.
24057866	1	47	dep	plasma	153:158	arg1	fucosylation					216:227	fucosylation	216:227	fucosylation	216:227	Our study compares the status of human seminal plasma immunoglobulin G (IgG) and IgA secretory component (SC) fucosylation between infertile leukocytospermic and normal, fertile normozoospermic patients.
24057866	1	47	dep	plasma	153:158	arg1	IgG					178:180	IgG	178:180	IgG	178:180	Our study compares the status of human seminal plasma immunoglobulin G (IgG) and IgA secretory component (SC) fucosylation between infertile leukocytospermic and normal, fertile normozoospermic patients.
24057866	0	48	theme	IgG	41:43	arg1	fucosylation					11:22	fucosylation	11:22	fucosylation of human seminal IgG	11:43	Changes in fucosylation of human seminal IgG and secretory component of IgA in leukocytospermic patients.
24057866	2	49	theme	seminal	314:320	arg1	IgG					322:324	The seminal IgG	310:324	The seminal IgG	310:324	The seminal IgG and SC are decorated with AAL-reactive core fucose, and antennary UEA- and LTA-reactive fucose of Lewis(y) and Lewis(x) structures, respectively.
24057866	1	50	theme	secretory	191:199	arg1	SC					212:213	SC	212:213	SC	212:213	Our study compares the status of human seminal plasma immunoglobulin G (IgG) and IgA secretory component (SC) fucosylation between infertile leukocytospermic and normal, fertile normozoospermic patients.
24057866	1	50	theme	secretory	191:199	arg1	component					201:209	IgA secretory component	187:209	IgA secretory component (SC)	187:214	Our study compares the status of human seminal plasma immunoglobulin G (IgG) and IgA secretory component (SC) fucosylation between infertile leukocytospermic and normal, fertile normozoospermic patients.
24057866	7	51	dep	linked	1098:1103	arg1	1-2					1080:1082	1-2	1080:1082	1-2	1080:1082	In leukocytospermia, the values of specific lectin reactivity for core fucose, fucose α(1-2)- and α(1-3)- linked, were similar for both SC bands.
24057866	7	51	dep	linked	1098:1103	arg1	α					1078:1078	fucose α	1071:1078	fucose α	1071:1078	In leukocytospermia, the values of specific lectin reactivity for core fucose, fucose α(1-2)- and α(1-3)- linked, were similar for both SC bands.
24057866	7	52	from	similar	1111:1117	arg1	leukocytospermia					995:1010	leukocytospermia	995:1010	leukocytospermia	995:1010	In leukocytospermia, the values of specific lectin reactivity for core fucose, fucose α(1-2)- and α(1-3)- linked, were similar for both SC bands.
24057866	9	53	theme	male	1603:1606	arg1	infertility					1608:1618	male infertility	1603:1618	male infertility associated with leukocytospermia with accompanying inflammation	1603:1682	The analysis of IgG and SC fucosylation status and the determination of IgG and S-IgA concentrations in seminal plasma might constitute a valuable diagnosis tools for the evaluation of male infertility associated with leukocytospermia with accompanying inflammation.
24057866	7	54	link	linked	1098:1103	arg1	similar					1111:1117	similar	1111:1117	similar	1111:1117	In leukocytospermia, the values of specific lectin reactivity for core fucose, fucose α(1-2)- and α(1-3)- linked, were similar for both SC bands.
24057866	7	54	link	linked	1098:1103	arg1	values					1017:1022	the values	1013:1022	the values	1013:1022	In leukocytospermia, the values of specific lectin reactivity for core fucose, fucose α(1-2)- and α(1-3)- linked, were similar for both SC bands.
24057866	6	55	theme	78-kDa	960:965	arg1	SC					967:968	78-kDa SC	960:968	78-kDa SC	960:968	The present study has also shown a higher AAL and LTA specific reactivity of glycans expressed in 63-kDa SC, in comparison to 78-kDa SC, in the normal group.
24057866	8	56	theme	leukocytospermic	1184:1199	arg1	samples					1201:1207	leukocytospermic samples	1184:1207	leukocytospermic samples	1184:1207	Moreover, the present study has shown that in leukocytospermic samples the mean concentrations of IgG and S-IgA are twice as high (131.68 ± 102.6 mg/l and 36 ± 27 mg/l, respectively) as in the normal group (67.68 ± 29.2 mg/l; p < 0.02, and 19 ± 18 mg/l, p < 0.019, respectively).
24057866	4	57	from	samples	621:627	arg1	present					593:599	present	593:599	present	593:599	The IgG present in leukocytospermic samples is characterized by lower expression of core fucose than in the normal group (0.82 ± 0.3 AU and 1.2 ± 0.3 AU, respectively; p < 0.002).
24057866	9	58	theme	accompanying	1658:1669	arg1	inflammation					1671:1682	accompanying inflammation	1658:1682	accompanying inflammation	1658:1682	The analysis of IgG and SC fucosylation status and the determination of IgG and S-IgA concentrations in seminal plasma might constitute a valuable diagnosis tools for the evaluation of male infertility associated with leukocytospermia with accompanying inflammation.
24057866	1	59	theme	plasma	153:158	arg1	status					129:134	the status	125:134	the status of human seminal plasma immunoglobulin G (IgG) and IgA secretory component (SC) fucosylation between infertile leukocytospermic and normal, fertile normozoospermic patients	125:307	Our study compares the status of human seminal plasma immunoglobulin G (IgG) and IgA secretory component (SC) fucosylation between infertile leukocytospermic and normal, fertile normozoospermic patients.
24057866	9	60	theme	S-IgA	1498:1502	arg1	concentrations					1504:1517	IgG and S-IgA concentrations	1490:1517	IgG and S-IgA concentrations in seminal plasma	1490:1535	The analysis of IgG and SC fucosylation status and the determination of IgG and S-IgA concentrations in seminal plasma might constitute a valuable diagnosis tools for the evaluation of male infertility associated with leukocytospermia with accompanying inflammation.
24057866	8	61	from	group	1338:1342	arg1	mg/l					1284:1287	131.68 ± 102.6 mg/l	1269:1287	131.68 ± 102.6 mg/l	1269:1287	Moreover, the present study has shown that in leukocytospermic samples the mean concentrations of IgG and S-IgA are twice as high (131.68 ± 102.6 mg/l and 36 ± 27 mg/l, respectively) as in the normal group (67.68 ± 29.2 mg/l; p < 0.02, and 19 ± 18 mg/l, p < 0.019, respectively).
24057866	8	61	from	group	1338:1342	arg1	mg/l					1301:1304	36 ± 27 mg/l	1293:1304	36 ± 27 mg/l	1293:1304	Moreover, the present study has shown that in leukocytospermic samples the mean concentrations of IgG and S-IgA are twice as high (131.68 ± 102.6 mg/l and 36 ± 27 mg/l, respectively) as in the normal group (67.68 ± 29.2 mg/l; p < 0.02, and 19 ± 18 mg/l, p < 0.019, respectively).
24057866	8	61	from	group	1338:1342	arg1	concentrations					1218:1231	the mean concentrations	1209:1231	the mean concentrations of IgG and S-IgA	1209:1248	Moreover, the present study has shown that in leukocytospermic samples the mean concentrations of IgG and S-IgA are twice as high (131.68 ± 102.6 mg/l and 36 ± 27 mg/l, respectively) as in the normal group (67.68 ± 29.2 mg/l; p < 0.02, and 19 ± 18 mg/l, p < 0.019, respectively).
24057866	8	61	from	group	1338:1342	arg1	high					1263:1266	high	1263:1266	high	1263:1266	Moreover, the present study has shown that in leukocytospermic samples the mean concentrations of IgG and S-IgA are twice as high (131.68 ± 102.6 mg/l and 36 ± 27 mg/l, respectively) as in the normal group (67.68 ± 29.2 mg/l; p < 0.02, and 19 ± 18 mg/l, p < 0.019, respectively).
24057866	3	62	theme	core	507:510	arg1	fucosylation					512:523	IgG core fucosylation	503:523	IgG core fucosylation	503:523	However, a correlation between IgG core fucosylation and IgG concentration (r = -0.52; p < 0.0003) was observed.
24057866	3	63	dep	concentration	533:545	arg1	r = -0.52					548:556	r = -0.52	548:556	r = -0.52; p < 0.0003	548:568	However, a correlation between IgG core fucosylation and IgG concentration (r = -0.52; p < 0.0003) was observed.
24057866	9	64	from	analysis	1422:1429	arg1	plasma					1530:1535	seminal plasma	1522:1535	seminal plasma	1522:1535	The analysis of IgG and SC fucosylation status and the determination of IgG and S-IgA concentrations in seminal plasma might constitute a valuable diagnosis tools for the evaluation of male infertility associated with leukocytospermia with accompanying inflammation.
24057866	3	65	dep	r = -0.52	548:556	arg1	p < 0.0003					559:568	p < 0.0003	559:568	p < 0.0003	559:568	However, a correlation between IgG core fucosylation and IgG concentration (r = -0.52; p < 0.0003) was observed.
24057866	8	66	theme	IgG	1236:1238	arg1	mg/l					1284:1287	131.68 ± 102.6 mg/l	1269:1287	131.68 ± 102.6 mg/l	1269:1287	Moreover, the present study has shown that in leukocytospermic samples the mean concentrations of IgG and S-IgA are twice as high (131.68 ± 102.6 mg/l and 36 ± 27 mg/l, respectively) as in the normal group (67.68 ± 29.2 mg/l; p < 0.02, and 19 ± 18 mg/l, p < 0.019, respectively).
24057866	8	66	theme	IgG	1236:1238	arg1	mg/l					1301:1304	36 ± 27 mg/l	1293:1304	36 ± 27 mg/l	1293:1304	Moreover, the present study has shown that in leukocytospermic samples the mean concentrations of IgG and S-IgA are twice as high (131.68 ± 102.6 mg/l and 36 ± 27 mg/l, respectively) as in the normal group (67.68 ± 29.2 mg/l; p < 0.02, and 19 ± 18 mg/l, p < 0.019, respectively).
24057866	8	66	theme	IgG	1236:1238	arg1	high					1263:1266	high	1263:1266	high	1263:1266	Moreover, the present study has shown that in leukocytospermic samples the mean concentrations of IgG and S-IgA are twice as high (131.68 ± 102.6 mg/l and 36 ± 27 mg/l, respectively) as in the normal group (67.68 ± 29.2 mg/l; p < 0.02, and 19 ± 18 mg/l, p < 0.019, respectively).
24057866	8	66	theme	IgG	1236:1238	arg1	concentrations					1218:1231	the mean concentrations	1209:1231	the mean concentrations of IgG and S-IgA	1209:1248	Moreover, the present study has shown that in leukocytospermic samples the mean concentrations of IgG and S-IgA are twice as high (131.68 ± 102.6 mg/l and 36 ± 27 mg/l, respectively) as in the normal group (67.68 ± 29.2 mg/l; p < 0.02, and 19 ± 18 mg/l, p < 0.019, respectively).
24057866	8	67	from	samples	1201:1207	arg1	mg/l					1284:1287	131.68 ± 102.6 mg/l	1269:1287	131.68 ± 102.6 mg/l	1269:1287	Moreover, the present study has shown that in leukocytospermic samples the mean concentrations of IgG and S-IgA are twice as high (131.68 ± 102.6 mg/l and 36 ± 27 mg/l, respectively) as in the normal group (67.68 ± 29.2 mg/l; p < 0.02, and 19 ± 18 mg/l, p < 0.019, respectively).
24057866	8	67	from	samples	1201:1207	arg1	mg/l					1301:1304	36 ± 27 mg/l	1293:1304	36 ± 27 mg/l	1293:1304	Moreover, the present study has shown that in leukocytospermic samples the mean concentrations of IgG and S-IgA are twice as high (131.68 ± 102.6 mg/l and 36 ± 27 mg/l, respectively) as in the normal group (67.68 ± 29.2 mg/l; p < 0.02, and 19 ± 18 mg/l, p < 0.019, respectively).
24057866	8	67	from	samples	1201:1207	arg1	concentrations					1218:1231	the mean concentrations	1209:1231	the mean concentrations of IgG and S-IgA	1209:1248	Moreover, the present study has shown that in leukocytospermic samples the mean concentrations of IgG and S-IgA are twice as high (131.68 ± 102.6 mg/l and 36 ± 27 mg/l, respectively) as in the normal group (67.68 ± 29.2 mg/l; p < 0.02, and 19 ± 18 mg/l, p < 0.019, respectively).
24057866	8	67	from	samples	1201:1207	arg1	high					1263:1266	high	1263:1266	high	1263:1266	Moreover, the present study has shown that in leukocytospermic samples the mean concentrations of IgG and S-IgA are twice as high (131.68 ± 102.6 mg/l and 36 ± 27 mg/l, respectively) as in the normal group (67.68 ± 29.2 mg/l; p < 0.02, and 19 ± 18 mg/l, p < 0.019, respectively).
24057866	9	68	theme	fucosylation	1445:1456	arg1	status					1458:1463	SC fucosylation status	1442:1463	SC fucosylation status	1442:1463	The analysis of IgG and SC fucosylation status and the determination of IgG and S-IgA concentrations in seminal plasma might constitute a valuable diagnosis tools for the evaluation of male infertility associated with leukocytospermia with accompanying inflammation.
24057866	8	69	theme	S-IgA	1244:1248	arg1	mg/l					1284:1287	131.68 ± 102.6 mg/l	1269:1287	131.68 ± 102.6 mg/l	1269:1287	Moreover, the present study has shown that in leukocytospermic samples the mean concentrations of IgG and S-IgA are twice as high (131.68 ± 102.6 mg/l and 36 ± 27 mg/l, respectively) as in the normal group (67.68 ± 29.2 mg/l; p < 0.02, and 19 ± 18 mg/l, p < 0.019, respectively).
24057866	8	69	theme	S-IgA	1244:1248	arg1	mg/l					1301:1304	36 ± 27 mg/l	1293:1304	36 ± 27 mg/l	1293:1304	Moreover, the present study has shown that in leukocytospermic samples the mean concentrations of IgG and S-IgA are twice as high (131.68 ± 102.6 mg/l and 36 ± 27 mg/l, respectively) as in the normal group (67.68 ± 29.2 mg/l; p < 0.02, and 19 ± 18 mg/l, p < 0.019, respectively).
24057866	8	69	theme	S-IgA	1244:1248	arg1	high					1263:1266	high	1263:1266	high	1263:1266	Moreover, the present study has shown that in leukocytospermic samples the mean concentrations of IgG and S-IgA are twice as high (131.68 ± 102.6 mg/l and 36 ± 27 mg/l, respectively) as in the normal group (67.68 ± 29.2 mg/l; p < 0.02, and 19 ± 18 mg/l, p < 0.019, respectively).
24057866	8	69	theme	S-IgA	1244:1248	arg1	concentrations					1218:1231	the mean concentrations	1209:1231	the mean concentrations of IgG and S-IgA	1209:1248	Moreover, the present study has shown that in leukocytospermic samples the mean concentrations of IgG and S-IgA are twice as high (131.68 ± 102.6 mg/l and 36 ± 27 mg/l, respectively) as in the normal group (67.68 ± 29.2 mg/l; p < 0.02, and 19 ± 18 mg/l, p < 0.019, respectively).
24057866	7	70	theme	core	1058:1061	arg1	fucose					1063:1068	core fucose	1058:1068	core fucose	1058:1068	In leukocytospermia, the values of specific lectin reactivity for core fucose, fucose α(1-2)- and α(1-3)- linked, were similar for both SC bands.
24057866	4	71	theme	lower	649:653	arg1	expression					655:664	lower expression	649:664	lower expression of core fucose than in the normal group (0.82 ± 0.3 AU and 1.2 ± 0.3 AU, respectively; p < 0.002)	649:762	The IgG present in leukocytospermic samples is characterized by lower expression of core fucose than in the normal group (0.82 ± 0.3 AU and 1.2 ± 0.3 AU, respectively; p < 0.002).
24057866	6	72	theme	glycans	911:917	arg1	LTA					884:886	LTA	884:886	LTA	884:886	The present study has also shown a higher AAL and LTA specific reactivity of glycans expressed in 63-kDa SC, in comparison to 78-kDa SC, in the normal group.
24057866	6	72	theme	glycans	911:917	arg1	AAL					876:878	a higher AAL	867:878	a higher AAL	867:878	The present study has also shown a higher AAL and LTA specific reactivity of glycans expressed in 63-kDa SC, in comparison to 78-kDa SC, in the normal group.
24057866	6	73	theme	normal	978:983	arg1	group					985:989	the normal group	974:989	the normal group	974:989	The present study has also shown a higher AAL and LTA specific reactivity of glycans expressed in 63-kDa SC, in comparison to 78-kDa SC, in the normal group.
24057866	9	74	theme	IgG	1490:1492	arg1	concentrations					1504:1517	IgG and S-IgA concentrations	1490:1517	IgG and S-IgA concentrations in seminal plasma	1490:1535	The analysis of IgG and SC fucosylation status and the determination of IgG and S-IgA concentrations in seminal plasma might constitute a valuable diagnosis tools for the evaluation of male infertility associated with leukocytospermia with accompanying inflammation.
24057866	6	75	dep	AAL	876:878	arg1	reactivity					897:906	specific reactivity	888:906	specific reactivity	888:906	The present study has also shown a higher AAL and LTA specific reactivity of glycans expressed in 63-kDa SC, in comparison to 78-kDa SC, in the normal group.
24057866	0	76	theme	IgA	72:74	arg1	Changes					0:6	Changes	0:6	Changes in fucosylation of human seminal IgG	0:43	Changes in fucosylation of human seminal IgG and secretory component of IgA in leukocytospermic patients.
24057866	0	76	theme	IgA	72:74	arg1	component					59:67	secretory component	49:67	secretory component of IgA	49:74	Changes in fucosylation of human seminal IgG and secretory component of IgA in leukocytospermic patients.
24057866	1	77	theme	human	139:143	arg1	plasma					153:158	human seminal plasma immunoglobulin G (IgG) and IgA secretory component (SC) fucosylation	139:227	plasma	153:158	Our study compares the status of human seminal plasma immunoglobulin G (IgG) and IgA secretory component (SC) fucosylation between infertile leukocytospermic and normal, fertile normozoospermic patients.
24057866	2	78	theme	Lewis	437:441	arg1	fucose					370:375	AAL-reactive core fucose	352:375	AAL-reactive core fucose	352:375	The seminal IgG and SC are decorated with AAL-reactive core fucose, and antennary UEA- and LTA-reactive fucose of Lewis(y) and Lewis(x) structures, respectively.
24057866	2	78	theme	Lewis	437:441	arg1	UEA-					392:395	antennary UEA-	382:395	antennary UEA-	382:395	The seminal IgG and SC are decorated with AAL-reactive core fucose, and antennary UEA- and LTA-reactive fucose of Lewis(y) and Lewis(x) structures, respectively.
24057866	2	78	theme	Lewis	437:441	arg1	fucose					414:419	LTA-reactive fucose	401:419	LTA-reactive fucose	401:419	The seminal IgG and SC are decorated with AAL-reactive core fucose, and antennary UEA- and LTA-reactive fucose of Lewis(y) and Lewis(x) structures, respectively.
24057866	1	79	dep	leukocytospermic	247:262	arg1	fertile					276:282	fertile	276:282	fertile	276:282	Our study compares the status of human seminal plasma immunoglobulin G (IgG) and IgA secretory component (SC) fucosylation between infertile leukocytospermic and normal, fertile normozoospermic patients.
24057866	0	80	theme	leukocytospermic	79:94	arg1	patients					96:103	leukocytospermic patients	79:103	leukocytospermic patients	79:103	Changes in fucosylation of human seminal IgG and secretory component of IgA in leukocytospermic patients.
24057866	5	81	from	forms	808:812	arg1	present					793:799	present	793:799	present	793:799	In seminal plasma the SC is present in two forms: 78-kDa and 63-kDa.
24057866	5	81	from	forms	808:812	arg1	SC					787:788	the SC	783:788	the SC	783:788	In seminal plasma the SC is present in two forms: 78-kDa and 63-kDa.
24057866	4	82	theme	normal	693:698	arg1	group					700:704	the normal group	689:704	the normal group (0.82 ± 0.3 AU and 1.2 ± 0.3 AU, respectively; p < 0.002)	689:762	The IgG present in leukocytospermic samples is characterized by lower expression of core fucose than in the normal group (0.82 ± 0.3 AU and 1.2 ± 0.3 AU, respectively; p < 0.002).
24057866	4	82	theme	normal	693:698	arg1	AU					718:719	0.82 ± 0.3 AU	707:719	0.82 ± 0.3 AU	707:719	The IgG present in leukocytospermic samples is characterized by lower expression of core fucose than in the normal group (0.82 ± 0.3 AU and 1.2 ± 0.3 AU, respectively; p < 0.002).
24057866	4	82	theme	normal	693:698	arg1	AU					735:736	1.2 ± 0.3 AU	725:736	1.2 ± 0.3 AU	725:736	The IgG present in leukocytospermic samples is characterized by lower expression of core fucose than in the normal group (0.82 ± 0.3 AU and 1.2 ± 0.3 AU, respectively; p < 0.002).
24057866	7	83	theme	reactivity	1043:1052	arg1	similar					1111:1117	similar	1111:1117	similar	1111:1117	In leukocytospermia, the values of specific lectin reactivity for core fucose, fucose α(1-2)- and α(1-3)- linked, were similar for both SC bands.
24057866	7	83	theme	reactivity	1043:1052	arg1	values					1017:1022	the values	1013:1022	the values	1013:1022	In leukocytospermia, the values of specific lectin reactivity for core fucose, fucose α(1-2)- and α(1-3)- linked, were similar for both SC bands.
24057866	5	84	located	present	793:799	arg1	forms					808:812	two forms	804:812	two forms: 78-kDa and 63-kDa	804:831	In seminal plasma the SC is present in two forms: 78-kDa and 63-kDa.
24057866	5	84	located	present	793:799	arg2	SC					787:788	the SC	783:788	the SC	783:788	In seminal plasma the SC is present in two forms: 78-kDa and 63-kDa.
24057866	5	84	located	present	793:799	arg1	plasma					776:781	seminal plasma	768:781	seminal plasma	768:781	In seminal plasma the SC is present in two forms: 78-kDa and 63-kDa.
24057866	5	84	located	present	793:799	arg2	present					793:799	present	793:799	present	793:799	In seminal plasma the SC is present in two forms: 78-kDa and 63-kDa.
24057866	7	85	theme	specific	1027:1034	arg1	reactivity					1043:1052	specific lectin reactivity	1027:1052	specific lectin reactivity	1027:1052	In leukocytospermia, the values of specific lectin reactivity for core fucose, fucose α(1-2)- and α(1-3)- linked, were similar for both SC bands.
24057866	8	86	dep	mg/l	1358:1361	arg1	mg/l					1386:1389	p < 0.02, and 19 ± 18 mg/l	1364:1389	p < 0.02, and 19 ± 18 mg/l	1364:1389	Moreover, the present study has shown that in leukocytospermic samples the mean concentrations of IgG and S-IgA are twice as high (131.68 ± 102.6 mg/l and 36 ± 27 mg/l, respectively) as in the normal group (67.68 ± 29.2 mg/l; p < 0.02, and 19 ± 18 mg/l, p < 0.019, respectively).
24057866	2	87	dep	Lewis	424:428	arg1	structures					446:455	structures	446:455	structures	446:455	The seminal IgG and SC are decorated with AAL-reactive core fucose, and antennary UEA- and LTA-reactive fucose of Lewis(y) and Lewis(x) structures, respectively.
24057866	4	88	theme	fucose	674:679	arg1	expression					655:664	lower expression	649:664	lower expression of core fucose than in the normal group (0.82 ± 0.3 AU and 1.2 ± 0.3 AU, respectively; p < 0.002)	649:762	The IgG present in leukocytospermic samples is characterized by lower expression of core fucose than in the normal group (0.82 ± 0.3 AU and 1.2 ± 0.3 AU, respectively; p < 0.002).
24057866	2	89	theme	AAL-reactive	352:363	arg1	fucose					370:375	AAL-reactive core fucose	352:375	AAL-reactive core fucose	352:375	The seminal IgG and SC are decorated with AAL-reactive core fucose, and antennary UEA- and LTA-reactive fucose of Lewis(y) and Lewis(x) structures, respectively.
24057866	2	90	theme	LTA-reactive	401:412	arg1	fucose					414:419	LTA-reactive fucose	401:419	LTA-reactive fucose	401:419	The seminal IgG and SC are decorated with AAL-reactive core fucose, and antennary UEA- and LTA-reactive fucose of Lewis(y) and Lewis(x) structures, respectively.
24057866	0	91	theme	seminal	33:39	arg1	IgG					41:43	human seminal IgG	27:43	human seminal IgG	27:43	Changes in fucosylation of human seminal IgG and secretory component of IgA in leukocytospermic patients.
24057866	9	92	from	plasma	1530:1535	arg1	analysis					1422:1429	The analysis	1418:1429	The analysis of IgG and SC fucosylation status	1418:1463	The analysis of IgG and SC fucosylation status and the determination of IgG and S-IgA concentrations in seminal plasma might constitute a valuable diagnosis tools for the evaluation of male infertility associated with leukocytospermia with accompanying inflammation.
24057866	9	92	from	plasma	1530:1535	arg1	determination					1473:1485	the determination	1469:1485	the determination of IgG and S-IgA concentrations in seminal plasma	1469:1535	The analysis of IgG and SC fucosylation status and the determination of IgG and S-IgA concentrations in seminal plasma might constitute a valuable diagnosis tools for the evaluation of male infertility associated with leukocytospermia with accompanying inflammation.
24057866	1	93	theme	normozoospermic	284:298	arg1	patients					300:307	infertile leukocytospermic and normal, fertile normozoospermic patients	237:307	infertile leukocytospermic and normal, fertile normozoospermic patients	237:307	Our study compares the status of human seminal plasma immunoglobulin G (IgG) and IgA secretory component (SC) fucosylation between infertile leukocytospermic and normal, fertile normozoospermic patients.
24057866	8	94	from	high	1263:1266	arg1	group					1338:1342	the normal group	1327:1342	the normal group (67.68 ± 29.2 mg/l; p < 0.02, and 19 ± 18 mg/l, p < 0.019, respectively)	1327:1415	Moreover, the present study has shown that in leukocytospermic samples the mean concentrations of IgG and S-IgA are twice as high (131.68 ± 102.6 mg/l and 36 ± 27 mg/l, respectively) as in the normal group (67.68 ± 29.2 mg/l; p < 0.02, and 19 ± 18 mg/l, p < 0.019, respectively).
24057866	8	94	from	high	1263:1266	arg1	samples					1201:1207	leukocytospermic samples	1184:1207	leukocytospermic samples	1184:1207	Moreover, the present study has shown that in leukocytospermic samples the mean concentrations of IgG and S-IgA are twice as high (131.68 ± 102.6 mg/l and 36 ± 27 mg/l, respectively) as in the normal group (67.68 ± 29.2 mg/l; p < 0.02, and 19 ± 18 mg/l, p < 0.019, respectively).
24057866	0	95	gly	fucosylation	11:22	arg1	IgG					41:43	human seminal IgG	27:43	human seminal IgG	27:43	Changes in fucosylation of human seminal IgG and secretory component of IgA in leukocytospermic patients.
24057866	0	96	from	component	59:67	arg1	fucosylation					11:22	fucosylation	11:22	fucosylation of human seminal IgG	11:43	Changes in fucosylation of human seminal IgG and secretory component of IgA in leukocytospermic patients.
24057866	0	96	from	component	59:67	arg1	patients					96:103	leukocytospermic patients	79:103	leukocytospermic patients	79:103	Changes in fucosylation of human seminal IgG and secretory component of IgA in leukocytospermic patients.
24057866	7	97	theme	α	1090:1090	arg1	1-2					1080:1082	1-2	1080:1082	1-2	1080:1082	In leukocytospermia, the values of specific lectin reactivity for core fucose, fucose α(1-2)- and α(1-3)- linked, were similar for both SC bands.
24057866	7	97	theme	α	1090:1090	arg1	α					1078:1078	fucose α	1071:1078	fucose α	1071:1078	In leukocytospermia, the values of specific lectin reactivity for core fucose, fucose α(1-2)- and α(1-3)- linked, were similar for both SC bands.
24057866	1	98	theme	component	201:209	arg1	status					129:134	the status	125:134	the status of human seminal plasma immunoglobulin G (IgG) and IgA secretory component (SC) fucosylation between infertile leukocytospermic and normal, fertile normozoospermic patients	125:307	Our study compares the status of human seminal plasma immunoglobulin G (IgG) and IgA secretory component (SC) fucosylation between infertile leukocytospermic and normal, fertile normozoospermic patients.
24057866	1	99	theme	IgA	187:189	arg1	SC					212:213	SC	212:213	SC	212:213	Our study compares the status of human seminal plasma immunoglobulin G (IgG) and IgA secretory component (SC) fucosylation between infertile leukocytospermic and normal, fertile normozoospermic patients.
24057866	1	99	theme	IgA	187:189	arg1	component					201:209	IgA secretory component	187:209	IgA secretory component (SC)	187:214	Our study compares the status of human seminal plasma immunoglobulin G (IgG) and IgA secretory component (SC) fucosylation between infertile leukocytospermic and normal, fertile normozoospermic patients.
24057866	9	100	theme	infertility	1608:1618	arg1	evaluation					1589:1598	the evaluation	1585:1598	the evaluation of male infertility associated with leukocytospermia with accompanying inflammation	1585:1682	The analysis of IgG and SC fucosylation status and the determination of IgG and S-IgA concentrations in seminal plasma might constitute a valuable diagnosis tools for the evaluation of male infertility associated with leukocytospermia with accompanying inflammation.
24057866	8	101	theme	mean	1213:1216	arg1	mg/l					1284:1287	131.68 ± 102.6 mg/l	1269:1287	131.68 ± 102.6 mg/l	1269:1287	Moreover, the present study has shown that in leukocytospermic samples the mean concentrations of IgG and S-IgA are twice as high (131.68 ± 102.6 mg/l and 36 ± 27 mg/l, respectively) as in the normal group (67.68 ± 29.2 mg/l; p < 0.02, and 19 ± 18 mg/l, p < 0.019, respectively).
24057866	8	101	theme	mean	1213:1216	arg1	mg/l					1301:1304	36 ± 27 mg/l	1293:1304	36 ± 27 mg/l	1293:1304	Moreover, the present study has shown that in leukocytospermic samples the mean concentrations of IgG and S-IgA are twice as high (131.68 ± 102.6 mg/l and 36 ± 27 mg/l, respectively) as in the normal group (67.68 ± 29.2 mg/l; p < 0.02, and 19 ± 18 mg/l, p < 0.019, respectively).
24057866	8	101	theme	mean	1213:1216	arg1	high					1263:1266	high	1263:1266	high	1263:1266	Moreover, the present study has shown that in leukocytospermic samples the mean concentrations of IgG and S-IgA are twice as high (131.68 ± 102.6 mg/l and 36 ± 27 mg/l, respectively) as in the normal group (67.68 ± 29.2 mg/l; p < 0.02, and 19 ± 18 mg/l, p < 0.019, respectively).
24057866	8	101	theme	mean	1213:1216	arg1	concentrations					1218:1231	the mean concentrations	1209:1231	the mean concentrations of IgG and S-IgA	1209:1248	Moreover, the present study has shown that in leukocytospermic samples the mean concentrations of IgG and S-IgA are twice as high (131.68 ± 102.6 mg/l and 36 ± 27 mg/l, respectively) as in the normal group (67.68 ± 29.2 mg/l; p < 0.02, and 19 ± 18 mg/l, p < 0.019, respectively).
29205092	0	0	theme	tissue	117:122	arg1	applications					136:147	cartilage tissue engineering applications	107:147	cartilage tissue engineering applications	107:147	Biocompatibility of subcutaneously implanted marine macromolecules cross-linked bio-composite scaffold for cartilage tissue engineering applications.
29205092	8	1	theme	IL-6	1541:1544	arg1	expressions					1509:1519	the normalized mRNA expressions	1489:1519	the normalized mRNA expressions of NF-κB, TNF-α and IL-6	1489:1544	The modulatory effect of implanted scaffold over inflammatory and stress signaling cascades were confirmed by the normalized mRNA expressions of NF-κB, TNF-α and IL-6.
29205092	1	2	theme	innovative	193:202	arg1	biomaterials					204:215	innovative biomaterials	193:215	innovative biomaterials which support the invasion and proliferation of living cells for potential applications in tissue engineering and regenerative medicine	193:351	There is an intense interest in developing innovative biomaterials which support the invasion and proliferation of living cells for potential applications in tissue engineering and regenerative medicine.
29205092	8	3	theme	signaling	1452:1460	arg1	cascades					1462:1469	stress signaling cascades	1445:1469	stress signaling cascades	1445:1469	The modulatory effect of implanted scaffold over inflammatory and stress signaling cascades were confirmed by the normalized mRNA expressions of NF-κB, TNF-α and IL-6.
29205092	0	4	theme	cartilage	107:115	arg1	applications					136:147	cartilage tissue engineering applications	107:147	cartilage tissue engineering applications	107:147	Biocompatibility of subcutaneously implanted marine macromolecules cross-linked bio-composite scaffold for cartilage tissue engineering applications.
29205092	5	5	from	effects	944:950	arg1	systems					1002:1008	vital organ systems	990:1008	vital organ systems such as liver, kidney, etc.,	990:1037	The effects of implanted biomaterial scaffolds on vital organ systems such as liver, kidney, etc., have been studied by hematology and serum biochemistry.
29205092	5	5	from	effects	944:950	arg1	liver					1018:1022	liver	1018:1022	liver	1018:1022	The effects of implanted biomaterial scaffolds on vital organ systems such as liver, kidney, etc., have been studied by hematology and serum biochemistry.
29205092	4	6	theme	8th	837:839	arg1	weeks					841:845	1st, 4th, and 8th weeks	823:845	1st, 4th, and 8th weeks	823:845	Following the implantation, animals were sacrificed and the scaffolds were resected at 1st, 4th, and 8th weeks; the surrounding tissue along with the implant was removed to evaluate its biocompatibility.
29205092	1	7	theme	tissue	308:313	arg1	engineering					315:325	tissue engineering	308:325	tissue engineering	308:325	There is an intense interest in developing innovative biomaterials which support the invasion and proliferation of living cells for potential applications in tissue engineering and regenerative medicine.
29205092	3	8	theme	in	563:564	arg1	biocompatibility					571:586	biocompatibility	571:586	biocompatibility	571:586	The in vivo biocompatibility and toxicity of HACF scaffold were tested by comparing them with those of a biocompatible surgical metal implant (SMI) in a subcutaneous rat model.
29205092	2	9	theme	Present	354:360	arg1	study					362:366	Present study	354:366	Present study	354:366	Present study demonstrated the in vivo biocompatibility and toxicity of a macromolecules cross-linked biocomposite scaffold composed of hydroxyapatite, alginate, chitosan and fucoidan abbreviated as HACF.
29205092	10	10	theme	engineering	1793:1803	arg1	applications					1805:1816	cartilage tissue engineering applications	1776:1816	cartilage tissue engineering applications	1776:1816	Taken together, these results demonstrated that HACF biocomposite scaffold signifies its suitability for further research as a scaffold material for cartilage tissue engineering applications.
29205092	0	11	theme	engineering	124:134	arg1	applications					136:147	cartilage tissue engineering applications	107:147	cartilage tissue engineering applications	107:147	Biocompatibility of subcutaneously implanted marine macromolecules cross-linked bio-composite scaffold for cartilage tissue engineering applications.
29205092	6	12	theme	HACF	1216:1219	arg1	scaffold					1221:1228	HACF scaffold	1216:1228	HACF scaffold	1216:1228	The activities of pro-inflammatory marker enzymes such as COX, 5-LOX, 15-LOX, and NOS were normal in rats implanted with HACF scaffold.
29205092	6	13	theme	enzymes	1137:1143	arg1	activities					1099:1108	The activities	1095:1108	The activities of pro-inflammatory marker enzymes such as COX, 5-LOX, 15-LOX, and NOS	1095:1179	The activities of pro-inflammatory marker enzymes such as COX, 5-LOX, 15-LOX, and NOS were normal in rats implanted with HACF scaffold.
29205092	6	13	theme	enzymes	1137:1143	arg1	normal					1186:1191	normal	1186:1191	normal	1186:1191	The activities of pro-inflammatory marker enzymes such as COX, 5-LOX, 15-LOX, and NOS were normal in rats implanted with HACF scaffold.
29205092	3	14	from	implant	693:699	arg1	model					729:733	a subcutaneous rat model	710:733	a subcutaneous rat model	710:733	The in vivo biocompatibility and toxicity of HACF scaffold were tested by comparing them with those of a biocompatible surgical metal implant (SMI) in a subcutaneous rat model.
29205092	6	15	from	normal	1186:1191	arg1	rats					1196:1199	rats	1196:1199	rats implanted with HACF scaffold	1196:1228	The activities of pro-inflammatory marker enzymes such as COX, 5-LOX, 15-LOX, and NOS were normal in rats implanted with HACF scaffold.
29205092	6	16	theme	pro-inflammatory	1113:1128	arg1	15-LOX					1165:1170	15-LOX	1165:1170	15-LOX	1165:1170	The activities of pro-inflammatory marker enzymes such as COX, 5-LOX, 15-LOX, and NOS were normal in rats implanted with HACF scaffold.
29205092	6	16	theme	pro-inflammatory	1113:1128	arg1	NOS					1177:1179	NOS	1177:1179	NOS	1177:1179	The activities of pro-inflammatory marker enzymes such as COX, 5-LOX, 15-LOX, and NOS were normal in rats implanted with HACF scaffold.
29205092	6	16	theme	pro-inflammatory	1113:1128	arg1	5-LOX					1158:1162	5-LOX	1158:1162	5-LOX	1158:1162	The activities of pro-inflammatory marker enzymes such as COX, 5-LOX, 15-LOX, and NOS were normal in rats implanted with HACF scaffold.
29205092	6	16	theme	pro-inflammatory	1113:1128	arg1	enzymes					1137:1143	pro-inflammatory marker enzymes	1113:1143	pro-inflammatory marker enzymes such as COX, 5-LOX, 15-LOX, and NOS	1113:1179	The activities of pro-inflammatory marker enzymes such as COX, 5-LOX, 15-LOX, and NOS were normal in rats implanted with HACF scaffold.
29205092	6	16	theme	pro-inflammatory	1113:1128	arg1	COX					1153:1155	COX	1153:1155	COX	1153:1155	The activities of pro-inflammatory marker enzymes such as COX, 5-LOX, 15-LOX, and NOS were normal in rats implanted with HACF scaffold.
29205092	5	17	theme	scaffolds	977:985	arg1	effects					944:950	The effects	940:950	The effects of implanted biomaterial scaffolds on vital organ systems such as liver, kidney, etc.,	940:1037	The effects of implanted biomaterial scaffolds on vital organ systems such as liver, kidney, etc., have been studied by hematology and serum biochemistry.
29205092	4	18	theme	surrounding	852:862	arg1	tissue					864:869	the surrounding tissue	848:869	the surrounding tissue along with the implant	848:892	Following the implantation, animals were sacrificed and the scaffolds were resected at 1st, 4th, and 8th weeks; the surrounding tissue along with the implant was removed to evaluate its biocompatibility.
29205092	2	19	link	cross-linked	443:454	arg1	scaffold					469:476	a macromolecules cross-linked biocomposite scaffold	426:476	a macromolecules cross-linked biocomposite scaffold composed of hydroxyapatite, alginate, chitosan and fucoidan abbreviated as HACF	426:556	Present study demonstrated the in vivo biocompatibility and toxicity of a macromolecules cross-linked biocomposite scaffold composed of hydroxyapatite, alginate, chitosan and fucoidan abbreviated as HACF.
29205092	5	20	theme	vital	990:994	arg1	systems					1002:1008	vital organ systems	990:1008	vital organ systems such as liver, kidney, etc.,	990:1037	The effects of implanted biomaterial scaffolds on vital organ systems such as liver, kidney, etc., have been studied by hematology and serum biochemistry.
29205092	5	20	theme	vital	990:994	arg1	liver					1018:1022	liver	1018:1022	liver	1018:1022	The effects of implanted biomaterial scaffolds on vital organ systems such as liver, kidney, etc., have been studied by hematology and serum biochemistry.
29205092	6	21	from	rats	1196:1199	arg1	normal					1186:1191	normal	1186:1191	normal	1186:1191	The activities of pro-inflammatory marker enzymes such as COX, 5-LOX, 15-LOX, and NOS were normal in rats implanted with HACF scaffold.
29205092	6	21	from	rats	1196:1199	arg1	activities					1099:1108	The activities	1095:1108	The activities of pro-inflammatory marker enzymes such as COX, 5-LOX, 15-LOX, and NOS	1095:1179	The activities of pro-inflammatory marker enzymes such as COX, 5-LOX, 15-LOX, and NOS were normal in rats implanted with HACF scaffold.
29205092	10	22	theme	tissue	1786:1791	arg1	applications					1805:1816	cartilage tissue engineering applications	1776:1816	cartilage tissue engineering applications	1776:1816	Taken together, these results demonstrated that HACF biocomposite scaffold signifies its suitability for further research as a scaffold material for cartilage tissue engineering applications.
29205092	7	23	theme	Hematological	1231:1243	arg1	parameters					1245:1254	Hematological parameters	1231:1254	Hematological parameters	1231:1254	Hematological parameters, antioxidant and lipid peroxidation status were also found to be normal in implanted rats same as that of control and SMI.
29205092	2	24	theme	in	385:386	arg1	biocompatibility					393:408	biocompatibility	393:408	biocompatibility	393:408	Present study demonstrated the in vivo biocompatibility and toxicity of a macromolecules cross-linked biocomposite scaffold composed of hydroxyapatite, alginate, chitosan and fucoidan abbreviated as HACF.
29205092	2	25	dep	biocompatibility	393:408	arg1	the					381:383	the	381:383	the	381:383	Present study demonstrated the in vivo biocompatibility and toxicity of a macromolecules cross-linked biocomposite scaffold composed of hydroxyapatite, alginate, chitosan and fucoidan abbreviated as HACF.
29205092	2	26	theme	scaffold	469:476	arg1	toxicity					414:421	toxicity	414:421	toxicity	414:421	Present study demonstrated the in vivo biocompatibility and toxicity of a macromolecules cross-linked biocomposite scaffold composed of hydroxyapatite, alginate, chitosan and fucoidan abbreviated as HACF.
29205092	2	26	theme	scaffold	469:476	arg1	biocompatibility					393:408	biocompatibility	393:408	biocompatibility	393:408	Present study demonstrated the in vivo biocompatibility and toxicity of a macromolecules cross-linked biocomposite scaffold composed of hydroxyapatite, alginate, chitosan and fucoidan abbreviated as HACF.
29205092	1	27	theme	regenerative	331:342	arg1	medicine					344:351	regenerative medicine	331:351	regenerative medicine	331:351	There is an intense interest in developing innovative biomaterials which support the invasion and proliferation of living cells for potential applications in tissue engineering and regenerative medicine.
29205092	5	28	theme	organ	996:1000	arg1	systems					1002:1008	vital organ systems	990:1008	vital organ systems such as liver, kidney, etc.,	990:1037	The effects of implanted biomaterial scaffolds on vital organ systems such as liver, kidney, etc., have been studied by hematology and serum biochemistry.
29205092	5	28	theme	organ	996:1000	arg1	liver					1018:1022	liver	1018:1022	liver	1018:1022	The effects of implanted biomaterial scaffolds on vital organ systems such as liver, kidney, etc., have been studied by hematology and serum biochemistry.
29205092	4	29	theme	4th	828:830	arg1	weeks					841:845	1st, 4th, and 8th weeks	823:845	1st, 4th, and 8th weeks	823:845	Following the implantation, animals were sacrificed and the scaffolds were resected at 1st, 4th, and 8th weeks; the surrounding tissue along with the implant was removed to evaluate its biocompatibility.
29205092	2	30	theme	biocomposite	456:467	arg1	scaffold					469:476	a macromolecules cross-linked biocomposite scaffold	426:476	a macromolecules cross-linked biocomposite scaffold composed of hydroxyapatite, alginate, chitosan and fucoidan abbreviated as HACF	426:556	Present study demonstrated the in vivo biocompatibility and toxicity of a macromolecules cross-linked biocomposite scaffold composed of hydroxyapatite, alginate, chitosan and fucoidan abbreviated as HACF.
29205092	0	31	theme	implanted	35:43	arg1	macromolecules					52:65	subcutaneously implanted marine macromolecules	20:65	subcutaneously implanted marine macromolecules	20:65	Biocompatibility of subcutaneously implanted marine macromolecules cross-linked bio-composite scaffold for cartilage tissue engineering applications.
29205092	7	32	theme	lipid	1273:1277	arg1	peroxidation					1279:1290	lipid peroxidation	1273:1290	lipid peroxidation status	1273:1297	Hematological parameters, antioxidant and lipid peroxidation status were also found to be normal in implanted rats same as that of control and SMI.
29205092	4	33	theme	1st	823:825	arg1	weeks					841:845	1st, 4th, and 8th weeks	823:845	1st, 4th, and 8th weeks	823:845	Following the implantation, animals were sacrificed and the scaffolds were resected at 1st, 4th, and 8th weeks; the surrounding tissue along with the implant was removed to evaluate its biocompatibility.
29205092	9	34	theme	tissue	1599:1604	arg1	analysis					1569:1576	The histopathological analysis	1547:1576	The histopathological analysis of liver, kidney and tissue	1547:1604	The histopathological analysis of liver, kidney and tissue support our results.
29205092	10	35	theme	HACF	1675:1678	arg1	scaffold					1693:1700	HACF biocomposite scaffold	1675:1700	HACF biocomposite scaffold	1675:1700	Taken together, these results demonstrated that HACF biocomposite scaffold signifies its suitability for further research as a scaffold material for cartilage tissue engineering applications.
29205092	8	36	theme	TNF-α	1531:1535	arg1	expressions					1509:1519	the normalized mRNA expressions	1489:1519	the normalized mRNA expressions of NF-κB, TNF-α and IL-6	1489:1544	The modulatory effect of implanted scaffold over inflammatory and stress signaling cascades were confirmed by the normalized mRNA expressions of NF-κB, TNF-α and IL-6.
29205092	10	37	theme	scaffold	1754:1761	arg1	material					1763:1770	a scaffold material	1752:1770	a scaffold material for cartilage tissue engineering applications	1752:1816	Taken together, these results demonstrated that HACF biocomposite scaffold signifies its suitability for further research as a scaffold material for cartilage tissue engineering applications.
29205092	3	38	theme	subcutaneous	712:723	arg1	model					729:733	a subcutaneous rat model	710:733	a subcutaneous rat model	710:733	The in vivo biocompatibility and toxicity of HACF scaffold were tested by comparing them with those of a biocompatible surgical metal implant (SMI) in a subcutaneous rat model.
29205092	6	39	theme	marker	1130:1135	arg1	15-LOX					1165:1170	15-LOX	1165:1170	15-LOX	1165:1170	The activities of pro-inflammatory marker enzymes such as COX, 5-LOX, 15-LOX, and NOS were normal in rats implanted with HACF scaffold.
29205092	6	39	theme	marker	1130:1135	arg1	NOS					1177:1179	NOS	1177:1179	NOS	1177:1179	The activities of pro-inflammatory marker enzymes such as COX, 5-LOX, 15-LOX, and NOS were normal in rats implanted with HACF scaffold.
29205092	6	39	theme	marker	1130:1135	arg1	5-LOX					1158:1162	5-LOX	1158:1162	5-LOX	1158:1162	The activities of pro-inflammatory marker enzymes such as COX, 5-LOX, 15-LOX, and NOS were normal in rats implanted with HACF scaffold.
29205092	6	39	theme	marker	1130:1135	arg1	enzymes					1137:1143	pro-inflammatory marker enzymes	1113:1143	pro-inflammatory marker enzymes such as COX, 5-LOX, 15-LOX, and NOS	1113:1179	The activities of pro-inflammatory marker enzymes such as COX, 5-LOX, 15-LOX, and NOS were normal in rats implanted with HACF scaffold.
29205092	6	39	theme	marker	1130:1135	arg1	COX					1153:1155	COX	1153:1155	COX	1153:1155	The activities of pro-inflammatory marker enzymes such as COX, 5-LOX, 15-LOX, and NOS were normal in rats implanted with HACF scaffold.
29205092	10	40	theme	cartilage	1776:1784	arg1	applications					1805:1816	cartilage tissue engineering applications	1776:1816	cartilage tissue engineering applications	1776:1816	Taken together, these results demonstrated that HACF biocomposite scaffold signifies its suitability for further research as a scaffold material for cartilage tissue engineering applications.
29205092	3	41	theme	HACF	604:607	arg1	scaffold					609:616	HACF scaffold	604:616	HACF scaffold	604:616	The in vivo biocompatibility and toxicity of HACF scaffold were tested by comparing them with those of a biocompatible surgical metal implant (SMI) in a subcutaneous rat model.
29205092	0	42	theme	macromolecules	52:65	arg1	Biocompatibility					0:15	Biocompatibility	0:15	Biocompatibility of subcutaneously implanted marine macromolecules	0:65	Biocompatibility of subcutaneously implanted marine macromolecules cross-linked bio-composite scaffold for cartilage tissue engineering applications.
29205092	9	43	theme	liver	1581:1585	arg1	analysis					1569:1576	The histopathological analysis	1547:1576	The histopathological analysis of liver, kidney and tissue	1547:1604	The histopathological analysis of liver, kidney and tissue support our results.
29205092	3	44	theme	rat	725:727	arg1	model					729:733	a subcutaneous rat model	710:733	a subcutaneous rat model	710:733	The in vivo biocompatibility and toxicity of HACF scaffold were tested by comparing them with those of a biocompatible surgical metal implant (SMI) in a subcutaneous rat model.
29205092	3	45	theme	surgical	678:685	arg1	SMI					702:704	SMI	702:704	SMI	702:704	The in vivo biocompatibility and toxicity of HACF scaffold were tested by comparing them with those of a biocompatible surgical metal implant (SMI) in a subcutaneous rat model.
29205092	3	45	theme	surgical	678:685	arg1	implant					693:699	a biocompatible surgical metal implant	662:699	a biocompatible surgical metal implant (SMI) in a subcutaneous rat model	662:733	The in vivo biocompatibility and toxicity of HACF scaffold were tested by comparing them with those of a biocompatible surgical metal implant (SMI) in a subcutaneous rat model.
29205092	10	46	theme	biocomposite	1680:1691	arg1	scaffold					1693:1700	HACF biocomposite scaffold	1675:1700	HACF biocomposite scaffold	1675:1700	Taken together, these results demonstrated that HACF biocomposite scaffold signifies its suitability for further research as a scaffold material for cartilage tissue engineering applications.
29205092	0	47	theme	marine	45:50	arg1	macromolecules					52:65	subcutaneously implanted marine macromolecules	20:65	subcutaneously implanted marine macromolecules	20:65	Biocompatibility of subcutaneously implanted marine macromolecules cross-linked bio-composite scaffold for cartilage tissue engineering applications.
29205092	7	48	theme	same	1346:1349	arg1	rats					1341:1344	implanted rats	1331:1344	implanted rats same as that of control and SMI	1331:1376	Hematological parameters, antioxidant and lipid peroxidation status were also found to be normal in implanted rats same as that of control and SMI.
29205092	1	49	dep	invasion	235:242	arg1	the					231:233	the	231:233	the	231:233	There is an intense interest in developing innovative biomaterials which support the invasion and proliferation of living cells for potential applications in tissue engineering and regenerative medicine.
29205092	1	50	theme	living	265:270	arg1	cells					272:276	living cells	265:276	living cells	265:276	There is an intense interest in developing innovative biomaterials which support the invasion and proliferation of living cells for potential applications in tissue engineering and regenerative medicine.
29205092	0	51	theme	bio-composite	80:92	arg1	scaffold					94:101	bio-composite scaffold	80:101	bio-composite scaffold for cartilage tissue engineering applications	80:147	Biocompatibility of subcutaneously implanted marine macromolecules cross-linked bio-composite scaffold for cartilage tissue engineering applications.
29205092	8	52	theme	scaffold	1414:1421	arg1	effect					1394:1399	The modulatory effect	1379:1399	The modulatory effect of implanted scaffold over inflammatory and stress signaling cascades	1379:1469	The modulatory effect of implanted scaffold over inflammatory and stress signaling cascades were confirmed by the normalized mRNA expressions of NF-κB, TNF-α and IL-6.
29205092	5	53	theme	implanted	955:963	arg1	scaffolds					977:985	implanted biomaterial scaffolds	955:985	implanted biomaterial scaffolds	955:985	The effects of implanted biomaterial scaffolds on vital organ systems such as liver, kidney, etc., have been studied by hematology and serum biochemistry.
29205092	8	54	theme	NF-κB	1524:1528	arg1	expressions					1509:1519	the normalized mRNA expressions	1489:1519	the normalized mRNA expressions of NF-κB, TNF-α and IL-6	1489:1544	The modulatory effect of implanted scaffold over inflammatory and stress signaling cascades were confirmed by the normalized mRNA expressions of NF-κB, TNF-α and IL-6.
29205092	1	55	from	applications	292:303	arg1	engineering					315:325	tissue engineering	308:325	tissue engineering	308:325	There is an intense interest in developing innovative biomaterials which support the invasion and proliferation of living cells for potential applications in tissue engineering and regenerative medicine.
29205092	1	55	from	applications	292:303	arg1	medicine					344:351	regenerative medicine	331:351	regenerative medicine	331:351	There is an intense interest in developing innovative biomaterials which support the invasion and proliferation of living cells for potential applications in tissue engineering and regenerative medicine.
29205092	3	56	theme	biocompatible	664:676	arg1	SMI					702:704	SMI	702:704	SMI	702:704	The in vivo biocompatibility and toxicity of HACF scaffold were tested by comparing them with those of a biocompatible surgical metal implant (SMI) in a subcutaneous rat model.
29205092	3	56	theme	biocompatible	664:676	arg1	implant					693:699	a biocompatible surgical metal implant	662:699	a biocompatible surgical metal implant (SMI) in a subcutaneous rat model	662:733	The in vivo biocompatibility and toxicity of HACF scaffold were tested by comparing them with those of a biocompatible surgical metal implant (SMI) in a subcutaneous rat model.
29205092	7	57	from	normal	1321:1326	arg1	rats					1341:1344	implanted rats	1331:1344	implanted rats same as that of control and SMI	1331:1376	Hematological parameters, antioxidant and lipid peroxidation status were also found to be normal in implanted rats same as that of control and SMI.
29205092	5	58	theme	biomaterial	965:975	arg1	scaffolds					977:985	implanted biomaterial scaffolds	955:985	implanted biomaterial scaffolds	955:985	The effects of implanted biomaterial scaffolds on vital organ systems such as liver, kidney, etc., have been studied by hematology and serum biochemistry.
29205092	9	59	theme	histopathological	1551:1567	arg1	analysis					1569:1576	The histopathological analysis	1547:1576	The histopathological analysis of liver, kidney and tissue	1547:1604	The histopathological analysis of liver, kidney and tissue support our results.
29205092	7	60	theme	peroxidation	1279:1290	arg1	status					1292:1297	lipid peroxidation status	1273:1297	lipid peroxidation status	1273:1297	Hematological parameters, antioxidant and lipid peroxidation status were also found to be normal in implanted rats same as that of control and SMI.
29205092	5	61	theme	serum	1075:1079	arg1	biochemistry					1081:1092	serum biochemistry	1075:1092	serum biochemistry	1075:1092	The effects of implanted biomaterial scaffolds on vital organ systems such as liver, kidney, etc., have been studied by hematology and serum biochemistry.
29205092	3	62	theme	metal	687:691	arg1	SMI					702:704	SMI	702:704	SMI	702:704	The in vivo biocompatibility and toxicity of HACF scaffold were tested by comparing them with those of a biocompatible surgical metal implant (SMI) in a subcutaneous rat model.
29205092	3	62	theme	metal	687:691	arg1	implant					693:699	a biocompatible surgical metal implant	662:699	a biocompatible surgical metal implant (SMI) in a subcutaneous rat model	662:733	The in vivo biocompatibility and toxicity of HACF scaffold were tested by comparing them with those of a biocompatible surgical metal implant (SMI) in a subcutaneous rat model.
29205092	1	63	theme	intense	162:168	arg1	interest					170:177	an intense interest	159:177	an intense interest in developing innovative biomaterials which support the invasion and proliferation of living cells for potential applications in tissue engineering and regenerative medicine	159:351	There is an intense interest in developing innovative biomaterials which support the invasion and proliferation of living cells for potential applications in tissue engineering and regenerative medicine.
29205092	8	64	theme	modulatory	1383:1392	arg1	effect					1394:1399	The modulatory effect	1379:1399	The modulatory effect of implanted scaffold over inflammatory and stress signaling cascades	1379:1469	The modulatory effect of implanted scaffold over inflammatory and stress signaling cascades were confirmed by the normalized mRNA expressions of NF-κB, TNF-α and IL-6.
29205092	1	65	theme	cells	272:276	arg1	proliferation					248:260	proliferation	248:260	proliferation	248:260	There is an intense interest in developing innovative biomaterials which support the invasion and proliferation of living cells for potential applications in tissue engineering and regenerative medicine.
29205092	1	65	theme	cells	272:276	arg1	invasion					235:242	invasion	235:242	invasion	235:242	There is an intense interest in developing innovative biomaterials which support the invasion and proliferation of living cells for potential applications in tissue engineering and regenerative medicine.
29205092	8	66	theme	mRNA	1504:1507	arg1	expressions					1509:1519	the normalized mRNA expressions	1489:1519	the normalized mRNA expressions of NF-κB, TNF-α and IL-6	1489:1544	The modulatory effect of implanted scaffold over inflammatory and stress signaling cascades were confirmed by the normalized mRNA expressions of NF-κB, TNF-α and IL-6.
29205092	3	67	dep	in	563:564	arg1	vivo					566:569	vivo	566:569	vivo	566:569	The in vivo biocompatibility and toxicity of HACF scaffold were tested by comparing them with those of a biocompatible surgical metal implant (SMI) in a subcutaneous rat model.
29205092	3	68	theme	scaffold	609:616	arg1	biocompatibility					571:586	biocompatibility	571:586	biocompatibility	571:586	The in vivo biocompatibility and toxicity of HACF scaffold were tested by comparing them with those of a biocompatible surgical metal implant (SMI) in a subcutaneous rat model.
29205092	3	68	theme	scaffold	609:616	arg1	toxicity					592:599	toxicity	592:599	toxicity	592:599	The in vivo biocompatibility and toxicity of HACF scaffold were tested by comparing them with those of a biocompatible surgical metal implant (SMI) in a subcutaneous rat model.
29205092	7	69	theme	implanted	1331:1339	arg1	rats					1341:1344	implanted rats	1331:1344	implanted rats same as that of control and SMI	1331:1376	Hematological parameters, antioxidant and lipid peroxidation status were also found to be normal in implanted rats same as that of control and SMI.
29205092	2	70	dep	in	385:386	arg1	vivo					388:391	vivo	388:391	vivo	388:391	Present study demonstrated the in vivo biocompatibility and toxicity of a macromolecules cross-linked biocomposite scaffold composed of hydroxyapatite, alginate, chitosan and fucoidan abbreviated as HACF.
29205092	8	71	theme	normalized	1493:1502	arg1	expressions					1509:1519	the normalized mRNA expressions	1489:1519	the normalized mRNA expressions of NF-κB, TNF-α and IL-6	1489:1544	The modulatory effect of implanted scaffold over inflammatory and stress signaling cascades were confirmed by the normalized mRNA expressions of NF-κB, TNF-α and IL-6.
29205092	8	72	theme	stress	1445:1450	arg1	cascades					1462:1469	stress signaling cascades	1445:1469	stress signaling cascades	1445:1469	The modulatory effect of implanted scaffold over inflammatory and stress signaling cascades were confirmed by the normalized mRNA expressions of NF-κB, TNF-α and IL-6.
29205092	7	73	from	rats	1341:1344	arg1	normal					1321:1326	normal	1321:1326	normal	1321:1326	Hematological parameters, antioxidant and lipid peroxidation status were also found to be normal in implanted rats same as that of control and SMI.
29205092	2	74	theme	cross-linked	443:454	arg1	scaffold					469:476	a macromolecules cross-linked biocomposite scaffold	426:476	a macromolecules cross-linked biocomposite scaffold composed of hydroxyapatite, alginate, chitosan and fucoidan abbreviated as HACF	426:556	Present study demonstrated the in vivo biocompatibility and toxicity of a macromolecules cross-linked biocomposite scaffold composed of hydroxyapatite, alginate, chitosan and fucoidan abbreviated as HACF.
29205092	1	75	theme	potential	282:290	arg1	applications					292:303	potential applications	282:303	potential applications in tissue engineering and regenerative medicine	282:351	There is an intense interest in developing innovative biomaterials which support the invasion and proliferation of living cells for potential applications in tissue engineering and regenerative medicine.
29205092	9	76	theme	kidney	1588:1593	arg1	analysis					1569:1576	The histopathological analysis	1547:1576	The histopathological analysis of liver, kidney and tissue	1547:1604	The histopathological analysis of liver, kidney and tissue support our results.
29205092	4	77	from	weeks	841:845	arg1	resected					811:818	resected	811:818	resected	811:818	Following the implantation, animals were sacrificed and the scaffolds were resected at 1st, 4th, and 8th weeks; the surrounding tissue along with the implant was removed to evaluate its biocompatibility.
29205092	4	77	from	weeks	841:845	arg1	scaffolds					796:804	the scaffolds	792:804	the scaffolds	792:804	Following the implantation, animals were sacrificed and the scaffolds were resected at 1st, 4th, and 8th weeks; the surrounding tissue along with the implant was removed to evaluate its biocompatibility.
29205092	2	78	theme	macromolecules	428:441	arg1	scaffold					469:476	a macromolecules cross-linked biocomposite scaffold	426:476	a macromolecules cross-linked biocomposite scaffold composed of hydroxyapatite, alginate, chitosan and fucoidan abbreviated as HACF	426:556	Present study demonstrated the in vivo biocompatibility and toxicity of a macromolecules cross-linked biocomposite scaffold composed of hydroxyapatite, alginate, chitosan and fucoidan abbreviated as HACF.
29205092	8	79	theme	implanted	1404:1412	arg1	scaffold					1414:1421	implanted scaffold	1404:1421	implanted scaffold	1404:1421	The modulatory effect of implanted scaffold over inflammatory and stress signaling cascades were confirmed by the normalized mRNA expressions of NF-κB, TNF-α and IL-6.
29205092	10	80	theme	further	1732:1738	arg1	research					1740:1747	further research	1732:1747	further research	1732:1747	Taken together, these results demonstrated that HACF biocomposite scaffold signifies its suitability for further research as a scaffold material for cartilage tissue engineering applications.
29253970	0	0	theme	adhesive	84:91	arg1	peptide					93:99	an adhesive peptide	81:99	an adhesive peptide	81:99	Enhancing strength and toughness of cellulose nanofibril network structures with an adhesive peptide.
29253970	3	1	theme	adhesive	660:667	arg1	peptide					669:675	the adhesive peptide	656:675	the adhesive peptide	656:675	Composite films were prepared by solution casting from water suspensions of TO-CNF adsorbed with the adhesive peptide.
29253970	6	2	theme	tensile	1038:1044	arg1	tests					1046:1050	tensile tests	1038:1050	tensile tests	1038:1050	The mechanical properties of the composites were characterized by tensile tests and dynamic mechanical analysis (DMA).
29253970	2	3	theme	lysine	429:434	arg1	residues					440:447	three lysine (K) residues	423:447	three lysine (K) residues	423:447	In this work, an adhesive peptide consisting of just 11 amino acid residues with a hydrophobic core sequence of FLIVI (F - phenylalanine, L - leucine, I - isoleucine, V - valine) flanked by three lysine (K) residues was adsorbed to 2,2,6,6-Tetramethyl-1-piperidinyloxy radical (TEMPO) oxidized cellulose nanofibrils (TO-CNF).
29253970	8	4	theme	neat	1322:1325	arg1	TO-CNF					1327:1332	neat TO-CNF	1322:1332	neat TO-CNF	1322:1332	% adhesive peptide adsorbed onto TO-CNF, the composite showed a modulus of 12.5±1.4GPa, a tensile strength of 344.5±(15.3)MPa, and a strain to failure of 7.8±0.4%, which are 34.4%, 48.8%, and 23.8% higher than those for neat TO-CNF, respectively.
29253970	9	5	dep	fracture	1408:1415	arg1	to					1405:1406	to	1405:1406	to	1405:1406	This resulted in significantly improved toughness (work to fracture) for the composite, 77% higher than that for the neat TO-CNF.
29253970	2	6	with	residues	300:307	arg1	sequence					333:340	a hydrophobic core sequence	314:340	a hydrophobic core sequence of FLIVI (F - phenylalanine, L - leucine, I - isoleucine, V - valine) flanked by three lysine (K) residues	314:447	In this work, an adhesive peptide consisting of just 11 amino acid residues with a hydrophobic core sequence of FLIVI (F - phenylalanine, L - leucine, I - isoleucine, V - valine) flanked by three lysine (K) residues was adsorbed to 2,2,6,6-Tetramethyl-1-piperidinyloxy radical (TEMPO) oxidized cellulose nanofibrils (TO-CNF).
29253970	4	7	theme	atomic	750:755	arg1	microscopy					763:772	atomic force microscopy	750:772	atomic force microscopy (AFM)	750:778	The nanofibrils network structure of the composite was characterized by atomic force microscopy (AFM).
29253970	4	7	theme	atomic	750:755	arg1	AFM					775:777	AFM	775:777	AFM	775:777	The nanofibrils network structure of the composite was characterized by atomic force microscopy (AFM).
29253970	9	8	theme	neat	1466:1469	arg1	TO-CNF					1471:1476	the neat TO-CNF	1462:1476	the neat TO-CNF	1462:1476	This resulted in significantly improved toughness (work to fracture) for the composite, 77% higher than that for the neat TO-CNF.
29253970	2	9	theme	acid	295:298	arg1	residues					300:307	just 11 amino acid residues	281:307	just 11 amino acid residues with a hydrophobic core sequence of FLIVI (F - phenylalanine, L - leucine, I - isoleucine, V - valine) flanked by three lysine (K) residues	281:447	In this work, an adhesive peptide consisting of just 11 amino acid residues with a hydrophobic core sequence of FLIVI (F - phenylalanine, L - leucine, I - isoleucine, V - valine) flanked by three lysine (K) residues was adsorbed to 2,2,6,6-Tetramethyl-1-piperidinyloxy radical (TEMPO) oxidized cellulose nanofibrils (TO-CNF).
29253970	8	10	theme	%	1263:1263	arg1	failure					1245:1251	failure	1245:1251	failure of 7.8±0.4%, which are 34.4%, 48.8%, and 23.8% higher than those for neat TO-CNF, respectively	1245:1346	% adhesive peptide adsorbed onto TO-CNF, the composite showed a modulus of 12.5±1.4GPa, a tensile strength of 344.5±(15.3)MPa, and a strain to failure of 7.8±0.4%, which are 34.4%, 48.8%, and 23.8% higher than those for neat TO-CNF, respectively.
29253970	5	11	from	composites	817:826	arg1	structure					785:793	The structure	781:793	The structure of the peptide in the composites and the interactions between TO-CNF and the peptide	781:878	The structure of the peptide in the composites and the interactions between TO-CNF and the peptide were studied by Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD).
29253970	4	12	theme	composite	719:727	arg1	structure					702:710	The nanofibrils network structure	678:710	The nanofibrils network structure of the composite	678:727	The nanofibrils network structure of the composite was characterized by atomic force microscopy (AFM).
29253970	2	13	theme	amino	289:293	arg1	residues					300:307	just 11 amino acid residues	281:307	just 11 amino acid residues with a hydrophobic core sequence of FLIVI (F - phenylalanine, L - leucine, I - isoleucine, V - valine) flanked by three lysine (K) residues	281:447	In this work, an adhesive peptide consisting of just 11 amino acid residues with a hydrophobic core sequence of FLIVI (F - phenylalanine, L - leucine, I - isoleucine, V - valine) flanked by three lysine (K) residues was adsorbed to 2,2,6,6-Tetramethyl-1-piperidinyloxy radical (TEMPO) oxidized cellulose nanofibrils (TO-CNF).
29253970	1	14	theme	nanofibrils	141:151	arg1	structure					161:169	cellulose nanofibrils network structure	131:169	cellulose nanofibrils network structure	131:169	The mechanical properties of cellulose nanofibrils network structure are essential for their applications in functional materials.
29253970	8	15	theme	adhesive	1104:1111	arg1	peptide					1113:1119	% adhesive peptide	1102:1119	% adhesive peptide adsorbed onto TO-CNF, the composite	1102:1155	% adhesive peptide adsorbed onto TO-CNF, the composite showed a modulus of 12.5±1.4GPa, a tensile strength of 344.5±(15.3)MPa, and a strain to failure of 7.8±0.4%, which are 34.4%, 48.8%, and 23.8% higher than those for neat TO-CNF, respectively.
29253970	8	16	theme	344.5±	1212:1217	arg1	MPa					1224:1226	344.5±(15.3)MPa	1212:1226	344.5±(15.3)MPa	1212:1226	% adhesive peptide adsorbed onto TO-CNF, the composite showed a modulus of 12.5±1.4GPa, a tensile strength of 344.5±(15.3)MPa, and a strain to failure of 7.8±0.4%, which are 34.4%, 48.8%, and 23.8% higher than those for neat TO-CNF, respectively.
29253970	5	17	from	interactions	836:847	arg1	composites					817:826	the composites	813:826	the composites	813:826	The structure of the peptide in the composites and the interactions between TO-CNF and the peptide were studied by Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD).
29253970	8	18	theme	12.5±1.4GPa	1177:1187	arg1	strain					1235:1240	a strain	1233:1240	a strain to failure of 7.8±0.4%, which are 34.4%, 48.8%, and 23.8% higher than those for neat TO-CNF, respectively	1233:1346	% adhesive peptide adsorbed onto TO-CNF, the composite showed a modulus of 12.5±1.4GPa, a tensile strength of 344.5±(15.3)MPa, and a strain to failure of 7.8±0.4%, which are 34.4%, 48.8%, and 23.8% higher than those for neat TO-CNF, respectively.
29253970	8	18	theme	12.5±1.4GPa	1177:1187	arg1	modulus					1166:1172	a modulus	1164:1172	a modulus of 12.5±1.4GPa	1164:1187	% adhesive peptide adsorbed onto TO-CNF, the composite showed a modulus of 12.5±1.4GPa, a tensile strength of 344.5±(15.3)MPa, and a strain to failure of 7.8±0.4%, which are 34.4%, 48.8%, and 23.8% higher than those for neat TO-CNF, respectively.
29253970	8	18	theme	12.5±1.4GPa	1177:1187	arg1	strength					1200:1207	a tensile strength	1190:1207	a tensile strength of 344.5±(15.3)MPa	1190:1226	% adhesive peptide adsorbed onto TO-CNF, the composite showed a modulus of 12.5±1.4GPa, a tensile strength of 344.5±(15.3)MPa, and a strain to failure of 7.8±0.4%, which are 34.4%, 48.8%, and 23.8% higher than those for neat TO-CNF, respectively.
29253970	8	19	theme	%	1102:1102	arg1	peptide					1113:1119	% adhesive peptide	1102:1119	% adhesive peptide adsorbed onto TO-CNF, the composite	1102:1155	% adhesive peptide adsorbed onto TO-CNF, the composite showed a modulus of 12.5±1.4GPa, a tensile strength of 344.5±(15.3)MPa, and a strain to failure of 7.8±0.4%, which are 34.4%, 48.8%, and 23.8% higher than those for neat TO-CNF, respectively.
29253970	6	20	theme	mechanical	1064:1073	arg1	DMA					1085:1087	DMA	1085:1087	DMA	1085:1087	The mechanical properties of the composites were characterized by tensile tests and dynamic mechanical analysis (DMA).
29253970	6	20	theme	mechanical	1064:1073	arg1	analysis					1075:1082	dynamic mechanical analysis	1056:1082	dynamic mechanical analysis (DMA)	1056:1088	The mechanical properties of the composites were characterized by tensile tests and dynamic mechanical analysis (DMA).
29253970	9	21	theme	work	1400:1403	arg1	fracture					1408:1415	work to fracture	1400:1415	work to fracture	1400:1415	This resulted in significantly improved toughness (work to fracture) for the composite, 77% higher than that for the neat TO-CNF.
29253970	9	21	theme	work	1400:1403	arg1	toughness					1389:1397	significantly improved toughness	1366:1397	significantly improved toughness (work to fracture) for the composite, 77% higher than that for the neat TO-CNF	1366:1476	This resulted in significantly improved toughness (work to fracture) for the composite, 77% higher than that for the neat TO-CNF.
29253970	2	22	theme	hydrophobic	316:326	arg1	sequence					333:340	a hydrophobic core sequence	314:340	a hydrophobic core sequence of FLIVI (F - phenylalanine, L - leucine, I - isoleucine, V - valine) flanked by three lysine (K) residues	314:447	In this work, an adhesive peptide consisting of just 11 amino acid residues with a hydrophobic core sequence of FLIVI (F - phenylalanine, L - leucine, I - isoleucine, V - valine) flanked by three lysine (K) residues was adsorbed to 2,2,6,6-Tetramethyl-1-piperidinyloxy radical (TEMPO) oxidized cellulose nanofibrils (TO-CNF).
29253970	6	23	theme	composites	1005:1014	arg1	properties					987:996	The mechanical properties	972:996	The mechanical properties of the composites	972:1014	The mechanical properties of the composites were characterized by tensile tests and dynamic mechanical analysis (DMA).
29253970	9	24	theme	higher	1441:1446	arg1	composite					1426:1434	the composite	1422:1434	the composite	1422:1434	This resulted in significantly improved toughness (work to fracture) for the composite, 77% higher than that for the neat TO-CNF.
29253970	4	25	theme	network	694:700	arg1	structure					702:710	The nanofibrils network structure	678:710	The nanofibrils network structure of the composite	678:727	The nanofibrils network structure of the composite was characterized by atomic force microscopy (AFM).
29253970	5	26	dep	transform	904:912	arg1	infrared					914:921	infrared	914:921	transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD)	904:969	The structure of the peptide in the composites and the interactions between TO-CNF and the peptide were studied by Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD).
29253970	5	27	theme	X-ray	947:951	arg1	XRD					966:968	XRD	966:968	XRD	966:968	The structure of the peptide in the composites and the interactions between TO-CNF and the peptide were studied by Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD).
29253970	5	27	theme	X-ray	947:951	arg1	diffraction					953:963	X-ray diffraction	947:963	X-ray diffraction (XRD)	947:969	The structure of the peptide in the composites and the interactions between TO-CNF and the peptide were studied by Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD).
29253970	2	28	dep	FLIVI	345:349	arg1	F					352:352	F	352:352	F	352:352	In this work, an adhesive peptide consisting of just 11 amino acid residues with a hydrophobic core sequence of FLIVI (F - phenylalanine, L - leucine, I - isoleucine, V - valine) flanked by three lysine (K) residues was adsorbed to 2,2,6,6-Tetramethyl-1-piperidinyloxy radical (TEMPO) oxidized cellulose nanofibrils (TO-CNF).
29253970	1	29	theme	functional	211:220	arg1	materials					222:230	functional materials	211:230	functional materials	211:230	The mechanical properties of cellulose nanofibrils network structure are essential for their applications in functional materials.
29253970	6	30	theme	dynamic	1056:1062	arg1	DMA					1085:1087	DMA	1085:1087	DMA	1085:1087	The mechanical properties of the composites were characterized by tensile tests and dynamic mechanical analysis (DMA).
29253970	6	30	theme	dynamic	1056:1062	arg1	analysis					1075:1082	dynamic mechanical analysis	1056:1082	dynamic mechanical analysis (DMA)	1056:1088	The mechanical properties of the composites were characterized by tensile tests and dynamic mechanical analysis (DMA).
29253970	5	31	from	structure	785:793	arg1	composites					817:826	the composites	813:826	the composites	813:826	The structure of the peptide in the composites and the interactions between TO-CNF and the peptide were studied by Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD).
29253970	1	32	theme	mechanical	106:115	arg1	properties					117:126	The mechanical properties	102:126	The mechanical properties of cellulose nanofibrils network structure	102:169	The mechanical properties of cellulose nanofibrils network structure are essential for their applications in functional materials.
29253970	1	32	theme	mechanical	106:115	arg1	essential					175:183	essential	175:183	essential	175:183	The mechanical properties of cellulose nanofibrils network structure are essential for their applications in functional materials.
29253970	5	33	dep	Fourier	896:902	arg1	transform					904:912	transform	904:912	transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD)	904:969	The structure of the peptide in the composites and the interactions between TO-CNF and the peptide were studied by Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD).
29253970	0	34	theme	cellulose	36:44	arg1	structures					65:74	cellulose nanofibril network structures	36:74	cellulose nanofibril network structures	36:74	Enhancing strength and toughness of cellulose nanofibril network structures with an adhesive peptide.
29253970	2	35	theme	radical	502:508	arg1	TO-CNF					550:555	TO-CNF	550:555	TO-CNF	550:555	In this work, an adhesive peptide consisting of just 11 amino acid residues with a hydrophobic core sequence of FLIVI (F - phenylalanine, L - leucine, I - isoleucine, V - valine) flanked by three lysine (K) residues was adsorbed to 2,2,6,6-Tetramethyl-1-piperidinyloxy radical (TEMPO) oxidized cellulose nanofibrils (TO-CNF).
29253970	2	35	theme	radical	502:508	arg1	nanofibrils					537:547	2,2,6,6-Tetramethyl-1-piperidinyloxy radical (TEMPO) oxidized cellulose nanofibrils	465:547	2,2,6,6-Tetramethyl-1-piperidinyloxy radical (TEMPO) oxidized cellulose nanofibrils (TO-CNF)	465:556	In this work, an adhesive peptide consisting of just 11 amino acid residues with a hydrophobic core sequence of FLIVI (F - phenylalanine, L - leucine, I - isoleucine, V - valine) flanked by three lysine (K) residues was adsorbed to 2,2,6,6-Tetramethyl-1-piperidinyloxy radical (TEMPO) oxidized cellulose nanofibrils (TO-CNF).
29253970	8	36	theme	MPa	1224:1226	arg1	strain					1235:1240	a strain	1233:1240	a strain to failure of 7.8±0.4%, which are 34.4%, 48.8%, and 23.8% higher than those for neat TO-CNF, respectively	1233:1346	% adhesive peptide adsorbed onto TO-CNF, the composite showed a modulus of 12.5±1.4GPa, a tensile strength of 344.5±(15.3)MPa, and a strain to failure of 7.8±0.4%, which are 34.4%, 48.8%, and 23.8% higher than those for neat TO-CNF, respectively.
29253970	8	36	theme	MPa	1224:1226	arg1	modulus					1166:1172	a modulus	1164:1172	a modulus of 12.5±1.4GPa	1164:1187	% adhesive peptide adsorbed onto TO-CNF, the composite showed a modulus of 12.5±1.4GPa, a tensile strength of 344.5±(15.3)MPa, and a strain to failure of 7.8±0.4%, which are 34.4%, 48.8%, and 23.8% higher than those for neat TO-CNF, respectively.
29253970	8	36	theme	MPa	1224:1226	arg1	strength					1200:1207	a tensile strength	1190:1207	a tensile strength of 344.5±(15.3)MPa	1190:1226	% adhesive peptide adsorbed onto TO-CNF, the composite showed a modulus of 12.5±1.4GPa, a tensile strength of 344.5±(15.3)MPa, and a strain to failure of 7.8±0.4%, which are 34.4%, 48.8%, and 23.8% higher than those for neat TO-CNF, respectively.
29253970	6	37	theme	mechanical	976:985	arg1	properties					987:996	The mechanical properties	972:996	The mechanical properties of the composites	972:1014	The mechanical properties of the composites were characterized by tensile tests and dynamic mechanical analysis (DMA).
29253970	2	38	theme	2,2,6,6-Tetramethyl-1-piperidinyloxy	465:500	arg1	TEMPO					511:515	TEMPO	511:515	TEMPO	511:515	In this work, an adhesive peptide consisting of just 11 amino acid residues with a hydrophobic core sequence of FLIVI (F - phenylalanine, L - leucine, I - isoleucine, V - valine) flanked by three lysine (K) residues was adsorbed to 2,2,6,6-Tetramethyl-1-piperidinyloxy radical (TEMPO) oxidized cellulose nanofibrils (TO-CNF).
29253970	2	38	theme	2,2,6,6-Tetramethyl-1-piperidinyloxy	465:500	arg1	radical					502:508	2,2,6,6-Tetramethyl-1-piperidinyloxy radical	465:508	2,2,6,6-Tetramethyl-1-piperidinyloxy radical (TEMPO) oxidized cellulose nanofibrils (TO-CNF)	465:556	In this work, an adhesive peptide consisting of just 11 amino acid residues with a hydrophobic core sequence of FLIVI (F - phenylalanine, L - leucine, I - isoleucine, V - valine) flanked by three lysine (K) residues was adsorbed to 2,2,6,6-Tetramethyl-1-piperidinyloxy radical (TEMPO) oxidized cellulose nanofibrils (TO-CNF).
29253970	0	39	with	toughness	23:31	arg1	peptide					93:99	an adhesive peptide	81:99	an adhesive peptide	81:99	Enhancing strength and toughness of cellulose nanofibril network structures with an adhesive peptide.
29253970	5	40	theme	peptide	802:808	arg1	structure					785:793	The structure	781:793	The structure of the peptide in the composites and the interactions between TO-CNF and the peptide	781:878	The structure of the peptide in the composites and the interactions between TO-CNF and the peptide were studied by Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD).
29253970	0	41	theme	network	57:63	arg1	structures					65:74	cellulose nanofibril network structures	36:74	cellulose nanofibril network structures	36:74	Enhancing strength and toughness of cellulose nanofibril network structures with an adhesive peptide.
29253970	3	42	theme	solution	592:599	arg1	casting					601:607	solution casting	592:607	solution casting	592:607	Composite films were prepared by solution casting from water suspensions of TO-CNF adsorbed with the adhesive peptide.
29253970	5	43	from	peptide	802:808	arg1	composites					817:826	the composites	813:826	the composites	813:826	The structure of the peptide in the composites and the interactions between TO-CNF and the peptide were studied by Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD).
29253970	2	44	theme	FLIVI	345:349	arg1	sequence					333:340	a hydrophobic core sequence	314:340	a hydrophobic core sequence of FLIVI (F - phenylalanine, L - leucine, I - isoleucine, V - valine) flanked by three lysine (K) residues	314:447	In this work, an adhesive peptide consisting of just 11 amino acid residues with a hydrophobic core sequence of FLIVI (F - phenylalanine, L - leucine, I - isoleucine, V - valine) flanked by three lysine (K) residues was adsorbed to 2,2,6,6-Tetramethyl-1-piperidinyloxy radical (TEMPO) oxidized cellulose nanofibrils (TO-CNF).
29253970	0	45	theme	nanofibril	46:55	arg1	structures					65:74	cellulose nanofibril network structures	36:74	cellulose nanofibril network structures	36:74	Enhancing strength and toughness of cellulose nanofibril network structures with an adhesive peptide.
29253970	2	46	theme	cellulose	527:535	arg1	TO-CNF					550:555	TO-CNF	550:555	TO-CNF	550:555	In this work, an adhesive peptide consisting of just 11 amino acid residues with a hydrophobic core sequence of FLIVI (F - phenylalanine, L - leucine, I - isoleucine, V - valine) flanked by three lysine (K) residues was adsorbed to 2,2,6,6-Tetramethyl-1-piperidinyloxy radical (TEMPO) oxidized cellulose nanofibrils (TO-CNF).
29253970	2	46	theme	cellulose	527:535	arg1	nanofibrils					537:547	2,2,6,6-Tetramethyl-1-piperidinyloxy radical (TEMPO) oxidized cellulose nanofibrils	465:547	2,2,6,6-Tetramethyl-1-piperidinyloxy radical (TEMPO) oxidized cellulose nanofibrils (TO-CNF)	465:556	In this work, an adhesive peptide consisting of just 11 amino acid residues with a hydrophobic core sequence of FLIVI (F - phenylalanine, L - leucine, I - isoleucine, V - valine) flanked by three lysine (K) residues was adsorbed to 2,2,6,6-Tetramethyl-1-piperidinyloxy radical (TEMPO) oxidized cellulose nanofibrils (TO-CNF).
29253970	9	47	theme	improved	1380:1387	arg1	fracture					1408:1415	work to fracture	1400:1415	work to fracture	1400:1415	This resulted in significantly improved toughness (work to fracture) for the composite, 77% higher than that for the neat TO-CNF.
29253970	9	47	theme	improved	1380:1387	arg1	toughness					1389:1397	significantly improved toughness	1366:1397	significantly improved toughness (work to fracture) for the composite, 77% higher than that for the neat TO-CNF	1366:1476	This resulted in significantly improved toughness (work to fracture) for the composite, 77% higher than that for the neat TO-CNF.
29253970	0	48	with	strength	10:17	arg1	peptide					93:99	an adhesive peptide	81:99	an adhesive peptide	81:99	Enhancing strength and toughness of cellulose nanofibril network structures with an adhesive peptide.
29253970	2	49	theme	oxidized	518:525	arg1	TO-CNF					550:555	TO-CNF	550:555	TO-CNF	550:555	In this work, an adhesive peptide consisting of just 11 amino acid residues with a hydrophobic core sequence of FLIVI (F - phenylalanine, L - leucine, I - isoleucine, V - valine) flanked by three lysine (K) residues was adsorbed to 2,2,6,6-Tetramethyl-1-piperidinyloxy radical (TEMPO) oxidized cellulose nanofibrils (TO-CNF).
29253970	2	49	theme	oxidized	518:525	arg1	nanofibrils					537:547	2,2,6,6-Tetramethyl-1-piperidinyloxy radical (TEMPO) oxidized cellulose nanofibrils	465:547	2,2,6,6-Tetramethyl-1-piperidinyloxy radical (TEMPO) oxidized cellulose nanofibrils (TO-CNF)	465:556	In this work, an adhesive peptide consisting of just 11 amino acid residues with a hydrophobic core sequence of FLIVI (F - phenylalanine, L - leucine, I - isoleucine, V - valine) flanked by three lysine (K) residues was adsorbed to 2,2,6,6-Tetramethyl-1-piperidinyloxy radical (TEMPO) oxidized cellulose nanofibrils (TO-CNF).
29253970	3	50	theme	TO-CNF	635:640	arg1	suspensions					620:630	water suspensions	614:630	water suspensions of TO-CNF adsorbed with the adhesive peptide	614:675	Composite films were prepared by solution casting from water suspensions of TO-CNF adsorbed with the adhesive peptide.
29253970	4	51	theme	force	757:761	arg1	microscopy					763:772	atomic force microscopy	750:772	atomic force microscopy (AFM)	750:778	The nanofibrils network structure of the composite was characterized by atomic force microscopy (AFM).
29253970	4	51	theme	force	757:761	arg1	AFM					775:777	AFM	775:777	AFM	775:777	The nanofibrils network structure of the composite was characterized by atomic force microscopy (AFM).
29253970	1	52	theme	cellulose	131:139	arg1	structure					161:169	cellulose nanofibrils network structure	131:169	cellulose nanofibrils network structure	131:169	The mechanical properties of cellulose nanofibrils network structure are essential for their applications in functional materials.
29253970	8	53	theme	tensile	1192:1198	arg1	strength					1200:1207	a tensile strength	1190:1207	a tensile strength of 344.5±(15.3)MPa	1190:1226	% adhesive peptide adsorbed onto TO-CNF, the composite showed a modulus of 12.5±1.4GPa, a tensile strength of 344.5±(15.3)MPa, and a strain to failure of 7.8±0.4%, which are 34.4%, 48.8%, and 23.8% higher than those for neat TO-CNF, respectively.
29253970	3	54	theme	water	614:618	arg1	suspensions					620:630	water suspensions	614:630	water suspensions of TO-CNF adsorbed with the adhesive peptide	614:675	Composite films were prepared by solution casting from water suspensions of TO-CNF adsorbed with the adhesive peptide.
29253970	2	55	dep	F	352:352	arg1	leucine					375:381	leucine	375:381	leucine	375:381	In this work, an adhesive peptide consisting of just 11 amino acid residues with a hydrophobic core sequence of FLIVI (F - phenylalanine, L - leucine, I - isoleucine, V - valine) flanked by three lysine (K) residues was adsorbed to 2,2,6,6-Tetramethyl-1-piperidinyloxy radical (TEMPO) oxidized cellulose nanofibrils (TO-CNF).
29253970	2	55	dep	F	352:352	arg1	V					400:400	V	400:400	V	400:400	In this work, an adhesive peptide consisting of just 11 amino acid residues with a hydrophobic core sequence of FLIVI (F - phenylalanine, L - leucine, I - isoleucine, V - valine) flanked by three lysine (K) residues was adsorbed to 2,2,6,6-Tetramethyl-1-piperidinyloxy radical (TEMPO) oxidized cellulose nanofibrils (TO-CNF).
29253970	2	55	dep	F	352:352	arg1	valine					404:409	valine	404:409	valine	404:409	In this work, an adhesive peptide consisting of just 11 amino acid residues with a hydrophobic core sequence of FLIVI (F - phenylalanine, L - leucine, I - isoleucine, V - valine) flanked by three lysine (K) residues was adsorbed to 2,2,6,6-Tetramethyl-1-piperidinyloxy radical (TEMPO) oxidized cellulose nanofibrils (TO-CNF).
29253970	2	55	dep	F	352:352	arg1	phenylalanine					356:368	phenylalanine	356:368	phenylalanine	356:368	In this work, an adhesive peptide consisting of just 11 amino acid residues with a hydrophobic core sequence of FLIVI (F - phenylalanine, L - leucine, I - isoleucine, V - valine) flanked by three lysine (K) residues was adsorbed to 2,2,6,6-Tetramethyl-1-piperidinyloxy radical (TEMPO) oxidized cellulose nanofibrils (TO-CNF).
29253970	2	55	dep	F	352:352	arg1	I					384:384	I	384:384	I	384:384	In this work, an adhesive peptide consisting of just 11 amino acid residues with a hydrophobic core sequence of FLIVI (F - phenylalanine, L - leucine, I - isoleucine, V - valine) flanked by three lysine (K) residues was adsorbed to 2,2,6,6-Tetramethyl-1-piperidinyloxy radical (TEMPO) oxidized cellulose nanofibrils (TO-CNF).
29253970	2	55	dep	F	352:352	arg1	isoleucine					388:397	isoleucine	388:397	isoleucine	388:397	In this work, an adhesive peptide consisting of just 11 amino acid residues with a hydrophobic core sequence of FLIVI (F - phenylalanine, L - leucine, I - isoleucine, V - valine) flanked by three lysine (K) residues was adsorbed to 2,2,6,6-Tetramethyl-1-piperidinyloxy radical (TEMPO) oxidized cellulose nanofibrils (TO-CNF).
29253970	2	55	dep	F	352:352	arg1	L					371:371	L	371:371	L	371:371	In this work, an adhesive peptide consisting of just 11 amino acid residues with a hydrophobic core sequence of FLIVI (F - phenylalanine, L - leucine, I - isoleucine, V - valine) flanked by three lysine (K) residues was adsorbed to 2,2,6,6-Tetramethyl-1-piperidinyloxy radical (TEMPO) oxidized cellulose nanofibrils (TO-CNF).
29253970	1	56	from	applications	195:206	arg1	materials					222:230	functional materials	211:230	functional materials	211:230	The mechanical properties of cellulose nanofibrils network structure are essential for their applications in functional materials.
29253970	2	57	theme	core	328:331	arg1	sequence					333:340	a hydrophobic core sequence	314:340	a hydrophobic core sequence of FLIVI (F - phenylalanine, L - leucine, I - isoleucine, V - valine) flanked by three lysine (K) residues	314:447	In this work, an adhesive peptide consisting of just 11 amino acid residues with a hydrophobic core sequence of FLIVI (F - phenylalanine, L - leucine, I - isoleucine, V - valine) flanked by three lysine (K) residues was adsorbed to 2,2,6,6-Tetramethyl-1-piperidinyloxy radical (TEMPO) oxidized cellulose nanofibrils (TO-CNF).
29253970	4	58	theme	nanofibrils	682:692	arg1	structure					702:710	The nanofibrils network structure	678:710	The nanofibrils network structure of the composite	678:727	The nanofibrils network structure of the composite was characterized by atomic force microscopy (AFM).
29253970	5	59	theme	interactions	836:847	arg1	structure					785:793	The structure	781:793	The structure of the peptide in the composites and the interactions between TO-CNF and the peptide	781:878	The structure of the peptide in the composites and the interactions between TO-CNF and the peptide were studied by Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD).
29253970	3	60	theme	Composite	559:567	arg1	films					569:573	Composite films	559:573	Composite films	559:573	Composite films were prepared by solution casting from water suspensions of TO-CNF adsorbed with the adhesive peptide.
29253970	1	61	theme	network	153:159	arg1	structure					161:169	cellulose nanofibrils network structure	131:169	cellulose nanofibrils network structure	131:169	The mechanical properties of cellulose nanofibrils network structure are essential for their applications in functional materials.
29253970	0	62	theme	structures	65:74	arg1	toughness					23:31	toughness	23:31	toughness	23:31	Enhancing strength and toughness of cellulose nanofibril network structures with an adhesive peptide.
29253970	0	62	theme	structures	65:74	arg1	strength					10:17	strength	10:17	strength	10:17	Enhancing strength and toughness of cellulose nanofibril network structures with an adhesive peptide.
29253970	2	63	theme	adhesive	250:257	arg1	peptide					259:265	an adhesive peptide	247:265	an adhesive peptide consisting of just 11 amino acid residues with a hydrophobic core sequence of FLIVI (F - phenylalanine, L - leucine, I - isoleucine, V - valine) flanked by three lysine (K) residues	247:447	In this work, an adhesive peptide consisting of just 11 amino acid residues with a hydrophobic core sequence of FLIVI (F - phenylalanine, L - leucine, I - isoleucine, V - valine) flanked by three lysine (K) residues was adsorbed to 2,2,6,6-Tetramethyl-1-piperidinyloxy radical (TEMPO) oxidized cellulose nanofibrils (TO-CNF).
29253970	1	64	theme	structure	161:169	arg1	properties					117:126	The mechanical properties	102:126	The mechanical properties of cellulose nanofibrils network structure	102:169	The mechanical properties of cellulose nanofibrils network structure are essential for their applications in functional materials.
29253970	1	64	theme	structure	161:169	arg1	essential					175:183	essential	175:183	essential	175:183	The mechanical properties of cellulose nanofibrils network structure are essential for their applications in functional materials.
28651046	5	0	theme	selective	812:820	arg1	de-sulfation					822:833	selective de-sulfation	812:833	selective de-sulfation	812:833	It is based on the use of modular disaccharides to assemble several selectively protected tetrasaccharides that were subjected to selective chemical modifications such as regioselective O- and N-sulfation and selective de-sulfation.
28651046	4	1	theme	synthetic	509:517	arg1	methodology					519:529	a synthetic methodology	507:529	a synthetic methodology that can rapidly provide a library of well-defined HS oligosaccharides	507:600	We describe here a synthetic methodology that can rapidly provide a library of well-defined HS oligosaccharides.
28651046	5	2	theme	several	663:669	arg1	tetrasaccharides					693:708	several selectively protected tetrasaccharides	663:708	several selectively protected tetrasaccharides that were subjected to selective chemical modifications such as regioselective O- and N-sulfation and selective de-sulfation	663:833	It is based on the use of modular disaccharides to assemble several selectively protected tetrasaccharides that were subjected to selective chemical modifications such as regioselective O- and N-sulfation and selective de-sulfation.
28651046	11	3	theme	biological	1704:1713	arg1	proliferation					1738:1750	cell proliferation	1733:1750	cell proliferation	1733:1750	The HS microarray data guided the selection of compounds that could interfere in biological processes such as cell proliferation.
28651046	11	3	theme	biological	1704:1713	arg1	processes					1715:1723	biological processes	1704:1723	biological processes such as cell proliferation	1704:1750	The HS microarray data guided the selection of compounds that could interfere in biological processes such as cell proliferation.
28651046	0	4	theme	Ligand	91:96	arg1	Requirements					98:109	Different Ligand Requirements	81:109	Different Ligand Requirements	81:109	Heparan Sulfate Microarray Reveals That Heparan Sulfate-Protein Binding Exhibits Different Ligand Requirements.
28651046	8	5	used	used	1266:1269	arg2	compounds					1251:1259	the resulting compounds	1237:1259	the resulting compounds	1237:1259	By employing tetrasaccharides with different backbone compositions, a library of 47 HS-oligosaccharides was prepared and the resulting compounds were used to construct a HS microarray.
28651046	11	6	theme	microarray	1630:1639	arg1	data					1641:1644	The HS microarray data	1623:1644	The HS microarray data	1623:1644	The HS microarray data guided the selection of compounds that could interfere in biological processes such as cell proliferation.
28651046	12	7	from	changes	1878:1884	arg1	composition					1905:1915	cell surface HS composition	1889:1915	cell surface HS composition	1889:1915	Although the library does not cover the entire chemical space of HS-tetrasaccharides, the binding data support a notion that changes in cell surface HS composition can modulate protein function.
28651046	12	8	theme	entire	1793:1798	arg1	space					1809:1813	the entire chemical space	1789:1813	the entire chemical space of HS-tetrasaccharides	1789:1836	Although the library does not cover the entire chemical space of HS-tetrasaccharides, the binding data support a notion that changes in cell surface HS composition can modulate protein function.
28651046	11	9	theme	cell	1733:1736	arg1	proliferation					1738:1750	cell proliferation	1733:1750	cell proliferation	1733:1750	The HS microarray data guided the selection of compounds that could interfere in biological processes such as cell proliferation.
28651046	5	10	theme	protected	683:691	arg1	tetrasaccharides					693:708	several selectively protected tetrasaccharides	663:708	several selectively protected tetrasaccharides that were subjected to selective chemical modifications such as regioselective O- and N-sulfation and selective de-sulfation	663:833	It is based on the use of modular disaccharides to assemble several selectively protected tetrasaccharides that were subjected to selective chemical modifications such as regioselective O- and N-sulfation and selective de-sulfation.
28651046	6	11	theme	compounds	862:870	arg1	number					838:843	A number	836:843	A number of the resulting compounds	836:870	A number of the resulting compounds were subjected to enzymatic modifications by 3-O-sulfotransferases-1 (3-OST1) to provide 3-O-sulfated derivatives.
28651046	12	12	theme	cell	1889:1892	arg1	composition					1905:1915	cell surface HS composition	1889:1915	cell surface HS composition	1889:1915	Although the library does not cover the entire chemical space of HS-tetrasaccharides, the binding data support a notion that changes in cell surface HS composition can modulate protein function.
28651046	3	13	theme	ligand	398:403	arg1	requirements					405:416	The ligand requirements	394:416	The ligand requirements of HS-binding proteins	394:439	The ligand requirements of HS-binding proteins have, however, been defined in only a few cases.
28651046	6	14	theme	3-O-sulfated	961:972	arg1	derivatives					974:984	3-O-sulfated derivatives	961:984	3-O-sulfated derivatives	961:984	A number of the resulting compounds were subjected to enzymatic modifications by 3-O-sulfotransferases-1 (3-OST1) to provide 3-O-sulfated derivatives.
28651046	6	15	theme	resulting	852:860	arg1	compounds					862:870	the resulting compounds	848:870	the resulting compounds	848:870	A number of the resulting compounds were subjected to enzymatic modifications by 3-O-sulfotransferases-1 (3-OST1) to provide 3-O-sulfated derivatives.
28651046	0	16	theme	Heparan	0:6	arg1	Microarray					16:25	Heparan Sulfate Microarray	0:25	Heparan Sulfate Microarray	0:25	Heparan Sulfate Microarray Reveals That Heparan Sulfate-Protein Binding Exhibits Different Ligand Requirements.
28651046	2	17	theme	protein	351:357	arg1	binding					359:365	protein binding	351:365	protein binding	351:365	Variations in HS epimerization and sulfation provide enormous structural diversity, which is believed to underpin protein binding and regulatory properties.
28651046	8	18	theme	HS	1286:1287	arg1	microarray					1289:1298	a HS microarray	1284:1298	a HS microarray	1284:1298	By employing tetrasaccharides with different backbone compositions, a library of 47 HS-oligosaccharides was prepared and the resulting compounds were used to construct a HS microarray.
28651046	5	19	theme	modular	629:635	arg1	disaccharides					637:649	modular disaccharides	629:649	modular disaccharides	629:649	It is based on the use of modular disaccharides to assemble several selectively protected tetrasaccharides that were subjected to selective chemical modifications such as regioselective O- and N-sulfation and selective de-sulfation.
28651046	9	20	theme	ligand	1305:1310	arg1	requirements					1312:1323	The ligand requirements	1301:1323	The ligand requirements of a number of HS-binding proteins including fibroblast growth factor 2 (FGF-2), and the chemokines CCL2, CCL5, CCL7, CCL13, CXCL8, and CXCL10	1301:1466	The ligand requirements of a number of HS-binding proteins including fibroblast growth factor 2 (FGF-2), and the chemokines CCL2, CCL5, CCL7, CCL13, CXCL8, and CXCL10 were examined using the array.
28651046	2	21	from	Variations	237:246	arg1	HS					251:252	HS epimerization and sulfation	251:280	HS epimerization and sulfation	251:280	Variations in HS epimerization and sulfation provide enormous structural diversity, which is believed to underpin protein binding and regulatory properties.
28651046	2	21	from	Variations	237:246	arg1	sulfation					272:280	sulfation	272:280	sulfation	272:280	Variations in HS epimerization and sulfation provide enormous structural diversity, which is believed to underpin protein binding and regulatory properties.
28651046	2	21	from	Variations	237:246	arg1	epimerization					254:266	epimerization	254:266	epimerization	254:266	Variations in HS epimerization and sulfation provide enormous structural diversity, which is believed to underpin protein binding and regulatory properties.
28651046	5	22	theme	disaccharides	637:649	arg1	use					622:624	the use	618:624	the use of modular disaccharides to assemble several selectively protected tetrasaccharides that were subjected to selective chemical modifications such as regioselective O- and N-sulfation and selective de-sulfation	618:833	It is based on the use of modular disaccharides to assemble several selectively protected tetrasaccharides that were subjected to selective chemical modifications such as regioselective O- and N-sulfation and selective de-sulfation.
28651046	2	23	theme	structural	299:308	arg1	diversity					310:318	enormous structural diversity	290:318	enormous structural diversity	290:318	Variations in HS epimerization and sulfation provide enormous structural diversity, which is believed to underpin protein binding and regulatory properties.
28651046	8	24	with	tetrasaccharides	1129:1144	arg1	compositions					1170:1181	different backbone compositions	1151:1181	different backbone compositions	1151:1181	By employing tetrasaccharides with different backbone compositions, a library of 47 HS-oligosaccharides was prepared and the resulting compounds were used to construct a HS microarray.
28651046	1	25	theme	wide	186:189	arg1	range					191:195	a wide range	184:195	a wide range of physiological and disease-processes	184:234	Heparan sulfates (HS) are linear sulfated polysaccharides that modulate a wide range of physiological and disease-processes.
28651046	0	26	theme	Sulfate	8:14	arg1	Microarray					16:25	Heparan Sulfate Microarray	0:25	Heparan Sulfate Microarray	0:25	Heparan Sulfate Microarray Reveals That Heparan Sulfate-Protein Binding Exhibits Different Ligand Requirements.
28651046	10	27	theme	multiple	1532:1539	arg1	compounds					1541:1549	multiple compounds	1532:1549	multiple compounds	1532:1549	Although all proteins recognized multiple compounds, they exhibited clear differences in structure-binding characteristics.
28651046	12	28	theme	chemical	1800:1807	arg1	space					1809:1813	the entire chemical space	1789:1813	the entire chemical space of HS-tetrasaccharides	1789:1836	Although the library does not cover the entire chemical space of HS-tetrasaccharides, the binding data support a notion that changes in cell surface HS composition can modulate protein function.
28651046	3	29	theme	proteins	432:439	arg1	requirements					405:416	The ligand requirements	394:416	The ligand requirements of HS-binding proteins	394:439	The ligand requirements of HS-binding proteins have, however, been defined in only a few cases.
28651046	4	30	theme	well-defined	569:580	arg1	oligosaccharides					585:600	well-defined HS oligosaccharides	569:600	well-defined HS oligosaccharides	569:600	We describe here a synthetic methodology that can rapidly provide a library of well-defined HS oligosaccharides.
28651046	1	31	theme	Heparan	112:118	arg1	polysaccharides					154:168	linear sulfated polysaccharides	138:168	linear sulfated polysaccharides that modulate a wide range of physiological and disease-processes	138:234	Heparan sulfates (HS) are linear sulfated polysaccharides that modulate a wide range of physiological and disease-processes.
28651046	1	31	theme	Heparan	112:118	arg1	HS					130:131	HS	130:131	HS	130:131	Heparan sulfates (HS) are linear sulfated polysaccharides that modulate a wide range of physiological and disease-processes.
28651046	1	31	theme	Heparan	112:118	arg1	sulfates					120:127	Heparan sulfates	112:127	Heparan sulfates (HS)	112:132	Heparan sulfates (HS) are linear sulfated polysaccharides that modulate a wide range of physiological and disease-processes.
28651046	5	32	theme	selective	733:741	arg1	O-					789:790	O-	789:790	O-	789:790	It is based on the use of modular disaccharides to assemble several selectively protected tetrasaccharides that were subjected to selective chemical modifications such as regioselective O- and N-sulfation and selective de-sulfation.
28651046	5	32	theme	selective	733:741	arg1	modifications					752:764	selective chemical modifications	733:764	selective chemical modifications such as regioselective O- and N-sulfation and selective de-sulfation	733:833	It is based on the use of modular disaccharides to assemble several selectively protected tetrasaccharides that were subjected to selective chemical modifications such as regioselective O- and N-sulfation and selective de-sulfation.
28651046	5	32	theme	selective	733:741	arg1	N-sulfation					796:806	N-sulfation	796:806	N-sulfation	796:806	It is based on the use of modular disaccharides to assemble several selectively protected tetrasaccharides that were subjected to selective chemical modifications such as regioselective O- and N-sulfation and selective de-sulfation.
28651046	5	32	theme	selective	733:741	arg1	de-sulfation					822:833	selective de-sulfation	812:833	selective de-sulfation	812:833	It is based on the use of modular disaccharides to assemble several selectively protected tetrasaccharides that were subjected to selective chemical modifications such as regioselective O- and N-sulfation and selective de-sulfation.
28651046	9	33	theme	number	1330:1335	arg1	requirements					1312:1323	The ligand requirements	1301:1323	The ligand requirements of a number of HS-binding proteins including fibroblast growth factor 2 (FGF-2), and the chemokines CCL2, CCL5, CCL7, CCL13, CXCL8, and CXCL10	1301:1466	The ligand requirements of a number of HS-binding proteins including fibroblast growth factor 2 (FGF-2), and the chemokines CCL2, CCL5, CCL7, CCL13, CXCL8, and CXCL10 were examined using the array.
28651046	1	34	theme	physiological	200:212	arg1	range					191:195	a wide range	184:195	a wide range of physiological and disease-processes	184:234	Heparan sulfates (HS) are linear sulfated polysaccharides that modulate a wide range of physiological and disease-processes.
28651046	4	35	theme	oligosaccharides	585:600	arg1	library					558:564	a library	556:564	a library of well-defined HS oligosaccharides	556:600	We describe here a synthetic methodology that can rapidly provide a library of well-defined HS oligosaccharides.
28651046	5	36	theme	chemical	743:750	arg1	O-					789:790	O-	789:790	O-	789:790	It is based on the use of modular disaccharides to assemble several selectively protected tetrasaccharides that were subjected to selective chemical modifications such as regioselective O- and N-sulfation and selective de-sulfation.
28651046	5	36	theme	chemical	743:750	arg1	modifications					752:764	selective chemical modifications	733:764	selective chemical modifications such as regioselective O- and N-sulfation and selective de-sulfation	733:833	It is based on the use of modular disaccharides to assemble several selectively protected tetrasaccharides that were subjected to selective chemical modifications such as regioselective O- and N-sulfation and selective de-sulfation.
28651046	5	36	theme	chemical	743:750	arg1	N-sulfation					796:806	N-sulfation	796:806	N-sulfation	796:806	It is based on the use of modular disaccharides to assemble several selectively protected tetrasaccharides that were subjected to selective chemical modifications such as regioselective O- and N-sulfation and selective de-sulfation.
28651046	5	36	theme	chemical	743:750	arg1	de-sulfation					822:833	selective de-sulfation	812:833	selective de-sulfation	812:833	It is based on the use of modular disaccharides to assemble several selectively protected tetrasaccharides that were subjected to selective chemical modifications such as regioselective O- and N-sulfation and selective de-sulfation.
28651046	0	37	theme	Heparan	40:46	arg1	Binding					64:70	Heparan Sulfate-Protein Binding	40:70	Heparan Sulfate-Protein Binding	40:70	Heparan Sulfate Microarray Reveals That Heparan Sulfate-Protein Binding Exhibits Different Ligand Requirements.
28651046	4	38	theme	HS	582:583	arg1	oligosaccharides					585:600	well-defined HS oligosaccharides	569:600	well-defined HS oligosaccharides	569:600	We describe here a synthetic methodology that can rapidly provide a library of well-defined HS oligosaccharides.
28651046	8	39	theme	HS-oligosaccharides	1200:1218	arg1	library					1186:1192	a library	1184:1192	a library of 47 HS-oligosaccharides	1184:1218	By employing tetrasaccharides with different backbone compositions, a library of 47 HS-oligosaccharides was prepared and the resulting compounds were used to construct a HS microarray.
28651046	9	40	theme	HS-binding	1340:1349	arg1	proteins					1351:1358	HS-binding proteins	1340:1358	HS-binding proteins including fibroblast growth factor 2 (FGF-2), and the chemokines CCL2, CCL5, CCL7, CCL13, CXCL8, and CXCL10	1340:1466	The ligand requirements of a number of HS-binding proteins including fibroblast growth factor 2 (FGF-2), and the chemokines CCL2, CCL5, CCL7, CCL13, CXCL8, and CXCL10 were examined using the array.
28651046	9	40	theme	HS-binding	1340:1349	arg1	factor					1388:1393	fibroblast growth factor 2	1370:1395	fibroblast growth factor 2 (FGF-2)	1370:1403	The ligand requirements of a number of HS-binding proteins including fibroblast growth factor 2 (FGF-2), and the chemokines CCL2, CCL5, CCL7, CCL13, CXCL8, and CXCL10 were examined using the array.
28651046	9	40	theme	HS-binding	1340:1349	arg1	chemokines					1414:1423	the chemokines CCL2, CCL5, CCL7, CCL13, CXCL8, and CXCL10	1410:1466	the chemokines CCL2, CCL5, CCL7, CCL13, CXCL8, and CXCL10	1410:1466	The ligand requirements of a number of HS-binding proteins including fibroblast growth factor 2 (FGF-2), and the chemokines CCL2, CCL5, CCL7, CCL13, CXCL8, and CXCL10 were examined using the array.
28651046	1	41	theme	disease-processes	218:234	arg1	range					191:195	a wide range	184:195	a wide range of physiological and disease-processes	184:234	Heparan sulfates (HS) are linear sulfated polysaccharides that modulate a wide range of physiological and disease-processes.
28651046	9	42	dep	chemokines	1414:1423	arg1	CXCL10					1461:1466	CXCL10	1461:1466	CXCL10	1461:1466	The ligand requirements of a number of HS-binding proteins including fibroblast growth factor 2 (FGF-2), and the chemokines CCL2, CCL5, CCL7, CCL13, CXCL8, and CXCL10 were examined using the array.
28651046	9	42	dep	chemokines	1414:1423	arg1	chemokines					1414:1423	the chemokines CCL2, CCL5, CCL7, CCL13, CXCL8, and CXCL10	1410:1466	the chemokines CCL2, CCL5, CCL7, CCL13, CXCL8, and CXCL10	1410:1466	The ligand requirements of a number of HS-binding proteins including fibroblast growth factor 2 (FGF-2), and the chemokines CCL2, CCL5, CCL7, CCL13, CXCL8, and CXCL10 were examined using the array.
28651046	9	42	dep	chemokines	1414:1423	arg1	CCL2					1425:1428	CCL2	1425:1428	CCL2	1425:1428	The ligand requirements of a number of HS-binding proteins including fibroblast growth factor 2 (FGF-2), and the chemokines CCL2, CCL5, CCL7, CCL13, CXCL8, and CXCL10 were examined using the array.
28651046	9	42	dep	chemokines	1414:1423	arg1	CCL13					1443:1447	CCL13	1443:1447	CCL13	1443:1447	The ligand requirements of a number of HS-binding proteins including fibroblast growth factor 2 (FGF-2), and the chemokines CCL2, CCL5, CCL7, CCL13, CXCL8, and CXCL10 were examined using the array.
28651046	9	42	dep	chemokines	1414:1423	arg1	CXCL8					1450:1454	CXCL8	1450:1454	CXCL8	1450:1454	The ligand requirements of a number of HS-binding proteins including fibroblast growth factor 2 (FGF-2), and the chemokines CCL2, CCL5, CCL7, CCL13, CXCL8, and CXCL10 were examined using the array.
28651046	9	42	dep	chemokines	1414:1423	arg1	CCL5					1431:1434	CCL5	1431:1434	CCL5	1431:1434	The ligand requirements of a number of HS-binding proteins including fibroblast growth factor 2 (FGF-2), and the chemokines CCL2, CCL5, CCL7, CCL13, CXCL8, and CXCL10 were examined using the array.
28651046	9	42	dep	chemokines	1414:1423	arg1	CCL7					1437:1440	CCL7	1437:1440	CCL7	1437:1440	The ligand requirements of a number of HS-binding proteins including fibroblast growth factor 2 (FGF-2), and the chemokines CCL2, CCL5, CCL7, CCL13, CXCL8, and CXCL10 were examined using the array.
28651046	11	43	theme	HS	1627:1628	arg1	data					1641:1644	The HS microarray data	1623:1644	The HS microarray data	1623:1644	The HS microarray data guided the selection of compounds that could interfere in biological processes such as cell proliferation.
28651046	9	44	theme	proteins	1351:1358	arg1	factor					1388:1393	fibroblast growth factor 2	1370:1395	fibroblast growth factor 2 (FGF-2)	1370:1403	The ligand requirements of a number of HS-binding proteins including fibroblast growth factor 2 (FGF-2), and the chemokines CCL2, CCL5, CCL7, CCL13, CXCL8, and CXCL10 were examined using the array.
28651046	9	44	theme	proteins	1351:1358	arg1	chemokines					1414:1423	the chemokines CCL2, CCL5, CCL7, CCL13, CXCL8, and CXCL10	1410:1466	the chemokines CCL2, CCL5, CCL7, CCL13, CXCL8, and CXCL10	1410:1466	The ligand requirements of a number of HS-binding proteins including fibroblast growth factor 2 (FGF-2), and the chemokines CCL2, CCL5, CCL7, CCL13, CXCL8, and CXCL10 were examined using the array.
28651046	9	44	theme	proteins	1351:1358	arg1	number					1330:1335	a number	1328:1335	a number of HS-binding proteins including fibroblast growth factor 2 (FGF-2), and the chemokines CCL2, CCL5, CCL7, CCL13, CXCL8, and CXCL10	1328:1466	The ligand requirements of a number of HS-binding proteins including fibroblast growth factor 2 (FGF-2), and the chemokines CCL2, CCL5, CCL7, CCL13, CXCL8, and CXCL10 were examined using the array.
28651046	10	45	from	differences	1573:1583	arg1	characteristics					1606:1620	structure-binding characteristics	1588:1620	structure-binding characteristics	1588:1620	Although all proteins recognized multiple compounds, they exhibited clear differences in structure-binding characteristics.
28651046	2	46	dep	HS	251:252	arg1	HS					251:252	HS epimerization and sulfation	251:280	HS epimerization and sulfation	251:280	Variations in HS epimerization and sulfation provide enormous structural diversity, which is believed to underpin protein binding and regulatory properties.
28651046	2	46	dep	HS	251:252	arg1	sulfation					272:280	sulfation	272:280	sulfation	272:280	Variations in HS epimerization and sulfation provide enormous structural diversity, which is believed to underpin protein binding and regulatory properties.
28651046	2	46	dep	HS	251:252	arg1	epimerization					254:266	epimerization	254:266	epimerization	254:266	Variations in HS epimerization and sulfation provide enormous structural diversity, which is believed to underpin protein binding and regulatory properties.
28651046	12	47	theme	HS	1902:1903	arg1	composition					1905:1915	cell surface HS composition	1889:1915	cell surface HS composition	1889:1915	Although the library does not cover the entire chemical space of HS-tetrasaccharides, the binding data support a notion that changes in cell surface HS composition can modulate protein function.
28651046	2	48	theme	regulatory	371:380	arg1	properties					382:391	regulatory properties	371:391	regulatory properties	371:391	Variations in HS epimerization and sulfation provide enormous structural diversity, which is believed to underpin protein binding and regulatory properties.
28651046	0	49	theme	Sulfate-Protein	48:62	arg1	Binding					64:70	Heparan Sulfate-Protein Binding	40:70	Heparan Sulfate-Protein Binding	40:70	Heparan Sulfate Microarray Reveals That Heparan Sulfate-Protein Binding Exhibits Different Ligand Requirements.
28651046	12	50	theme	protein	1930:1936	arg1	function					1938:1945	protein function	1930:1945	protein function	1930:1945	Although the library does not cover the entire chemical space of HS-tetrasaccharides, the binding data support a notion that changes in cell surface HS composition can modulate protein function.
28651046	9	51	theme	fibroblast	1370:1379	arg1	factor					1388:1393	fibroblast growth factor 2	1370:1395	fibroblast growth factor 2 (FGF-2)	1370:1403	The ligand requirements of a number of HS-binding proteins including fibroblast growth factor 2 (FGF-2), and the chemokines CCL2, CCL5, CCL7, CCL13, CXCL8, and CXCL10 were examined using the array.
28651046	9	51	theme	fibroblast	1370:1379	arg1	FGF-2					1398:1402	FGF-2	1398:1402	FGF-2	1398:1402	The ligand requirements of a number of HS-binding proteins including fibroblast growth factor 2 (FGF-2), and the chemokines CCL2, CCL5, CCL7, CCL13, CXCL8, and CXCL10 were examined using the array.
28651046	8	52	theme	backbone	1161:1168	arg1	compositions					1170:1181	different backbone compositions	1151:1181	different backbone compositions	1151:1181	By employing tetrasaccharides with different backbone compositions, a library of 47 HS-oligosaccharides was prepared and the resulting compounds were used to construct a HS microarray.
28651046	7	53	theme	sulfated	1094:1101	arg1	derivatives					1103:1113	12 differently sulfated derivatives	1079:1113	12 differently sulfated derivatives	1079:1113	The various approaches for diversification allowed one tetrasaccharide to be converted into 12 differently sulfated derivatives.
28651046	8	54	theme	different	1151:1159	arg1	compositions					1170:1181	different backbone compositions	1151:1181	different backbone compositions	1151:1181	By employing tetrasaccharides with different backbone compositions, a library of 47 HS-oligosaccharides was prepared and the resulting compounds were used to construct a HS microarray.
28651046	12	55	theme	binding	1843:1849	arg1	data					1851:1854	the binding data	1839:1854	the binding data	1839:1854	Although the library does not cover the entire chemical space of HS-tetrasaccharides, the binding data support a notion that changes in cell surface HS composition can modulate protein function.
28651046	10	56	theme	structure-binding	1588:1604	arg1	characteristics					1606:1620	structure-binding characteristics	1588:1620	structure-binding characteristics	1588:1620	Although all proteins recognized multiple compounds, they exhibited clear differences in structure-binding characteristics.
28651046	6	57	theme	enzymatic	890:898	arg1	modifications					900:912	enzymatic modifications	890:912	enzymatic modifications by 3-O-sulfotransferases-1 (3-OST1)	890:948	A number of the resulting compounds were subjected to enzymatic modifications by 3-O-sulfotransferases-1 (3-OST1) to provide 3-O-sulfated derivatives.
28651046	2	58	theme	enormous	290:297	arg1	diversity					310:318	enormous structural diversity	290:318	enormous structural diversity	290:318	Variations in HS epimerization and sulfation provide enormous structural diversity, which is believed to underpin protein binding and regulatory properties.
28651046	11	59	theme	compounds	1670:1678	arg1	selection					1657:1665	the selection	1653:1665	the selection of compounds that could interfere in biological processes such as cell proliferation	1653:1750	The HS microarray data guided the selection of compounds that could interfere in biological processes such as cell proliferation.
28651046	7	60	theme	various	991:997	arg1	approaches					999:1008	The various approaches	987:1008	The various approaches for diversification	987:1028	The various approaches for diversification allowed one tetrasaccharide to be converted into 12 differently sulfated derivatives.
28651046	1	61	theme	linear	138:143	arg1	polysaccharides					154:168	linear sulfated polysaccharides	138:168	linear sulfated polysaccharides that modulate a wide range of physiological and disease-processes	138:234	Heparan sulfates (HS) are linear sulfated polysaccharides that modulate a wide range of physiological and disease-processes.
28651046	1	61	theme	linear	138:143	arg1	sulfates					120:127	Heparan sulfates	112:127	Heparan sulfates (HS)	112:132	Heparan sulfates (HS) are linear sulfated polysaccharides that modulate a wide range of physiological and disease-processes.
28651046	12	62	theme	HS-tetrasaccharides	1818:1836	arg1	space					1809:1813	the entire chemical space	1789:1813	the entire chemical space of HS-tetrasaccharides	1789:1836	Although the library does not cover the entire chemical space of HS-tetrasaccharides, the binding data support a notion that changes in cell surface HS composition can modulate protein function.
28651046	0	63	theme	Different	81:89	arg1	Requirements					98:109	Different Ligand Requirements	81:109	Different Ligand Requirements	81:109	Heparan Sulfate Microarray Reveals That Heparan Sulfate-Protein Binding Exhibits Different Ligand Requirements.
28651046	1	64	theme	sulfated	145:152	arg1	polysaccharides					154:168	linear sulfated polysaccharides	138:168	linear sulfated polysaccharides that modulate a wide range of physiological and disease-processes	138:234	Heparan sulfates (HS) are linear sulfated polysaccharides that modulate a wide range of physiological and disease-processes.
28651046	1	64	theme	sulfated	145:152	arg1	sulfates					120:127	Heparan sulfates	112:127	Heparan sulfates (HS)	112:132	Heparan sulfates (HS) are linear sulfated polysaccharides that modulate a wide range of physiological and disease-processes.
28651046	9	65	theme	growth	1381:1386	arg1	factor					1388:1393	fibroblast growth factor 2	1370:1395	fibroblast growth factor 2 (FGF-2)	1370:1403	The ligand requirements of a number of HS-binding proteins including fibroblast growth factor 2 (FGF-2), and the chemokines CCL2, CCL5, CCL7, CCL13, CXCL8, and CXCL10 were examined using the array.
28651046	9	65	theme	growth	1381:1386	arg1	FGF-2					1398:1402	FGF-2	1398:1402	FGF-2	1398:1402	The ligand requirements of a number of HS-binding proteins including fibroblast growth factor 2 (FGF-2), and the chemokines CCL2, CCL5, CCL7, CCL13, CXCL8, and CXCL10 were examined using the array.
28651046	10	66	theme	clear	1567:1571	arg1	differences					1573:1583	clear differences	1567:1583	clear differences in structure-binding characteristics	1567:1620	Although all proteins recognized multiple compounds, they exhibited clear differences in structure-binding characteristics.
28651046	8	67	theme	resulting	1241:1249	arg1	compounds					1251:1259	the resulting compounds	1237:1259	the resulting compounds	1237:1259	By employing tetrasaccharides with different backbone compositions, a library of 47 HS-oligosaccharides was prepared and the resulting compounds were used to construct a HS microarray.
28651046	12	68	theme	surface	1894:1900	arg1	composition					1905:1915	cell surface HS composition	1889:1915	cell surface HS composition	1889:1915	Although the library does not cover the entire chemical space of HS-tetrasaccharides, the binding data support a notion that changes in cell surface HS composition can modulate protein function.
28651046	3	69	theme	HS-binding	421:430	arg1	proteins					432:439	HS-binding proteins	421:439	HS-binding proteins	421:439	The ligand requirements of HS-binding proteins have, however, been defined in only a few cases.
28651046	5	70	theme	regioselective	774:787	arg1	O-					789:790	O-	789:790	O-	789:790	It is based on the use of modular disaccharides to assemble several selectively protected tetrasaccharides that were subjected to selective chemical modifications such as regioselective O- and N-sulfation and selective de-sulfation.
24576958	1	0	with	fibrin	200:205	arg1	phosphate					228:236	beta-tricalcium phosphate	212:236	beta-tricalcium phosphate	212:236	The study presents comparative analysis of porous composites made of chitosan, alginate, fibrin with beta-tricalcium phosphate.
24576958	1	1	theme	composites	161:170	arg1	analysis					142:149	comparative analysis	130:149	comparative analysis of porous composites made of chitosan, alginate, fibrin with beta-tricalcium phosphate	130:236	The study presents comparative analysis of porous composites made of chitosan, alginate, fibrin with beta-tricalcium phosphate.
24576958	2	2	theme	fibrin-beta-TCP-based	296:316	arg1	composite					318:326	fibrin-beta-TCP-based composite	296:326	fibrin-beta-TCP-based composite	296:326	Histological findings on Wistar rat condyles showed that fibrin-beta-TCP-based composite had the most effective positive biological response.
24576958	1	3	theme	comparative	130:140	arg1	analysis					142:149	comparative analysis	130:149	comparative analysis of porous composites made of chitosan, alginate, fibrin with beta-tricalcium phosphate	130:236	The study presents comparative analysis of porous composites made of chitosan, alginate, fibrin with beta-tricalcium phosphate.
24576958	2	4	theme	Wistar	264:269	arg1	condyles					275:282	Wistar rat condyles	264:282	Wistar rat condyles	264:282	Histological findings on Wistar rat condyles showed that fibrin-beta-TCP-based composite had the most effective positive biological response.
24576958	1	5	theme	beta-tricalcium	212:226	arg1	phosphate					228:236	beta-tricalcium phosphate	212:236	beta-tricalcium phosphate	212:236	The study presents comparative analysis of porous composites made of chitosan, alginate, fibrin with beta-tricalcium phosphate.
24576958	2	6	theme	effective	341:349	arg1	response					371:378	the most effective positive biological response	332:378	the most effective positive biological response	332:378	Histological findings on Wistar rat condyles showed that fibrin-beta-TCP-based composite had the most effective positive biological response.
24576958	0	7	with	fibrin	76:81	arg1	phosphate					99:107	tricalcium phosphate	88:107	tricalcium phosphate	88:107	[Comparative study of osteoplastic materials based on chitosan, alginate or fibrin with tricalcium phosphate].
24576958	1	8	theme	porous	154:159	arg1	composites					161:170	porous composites	154:170	porous composites made of chitosan, alginate, fibrin with beta-tricalcium phosphate	154:236	The study presents comparative analysis of porous composites made of chitosan, alginate, fibrin with beta-tricalcium phosphate.
24576958	0	9	theme	materials	35:43	arg1	study					13:17	[Comparative study	0:17	[Comparative study of osteoplastic materials	0:43	[Comparative study of osteoplastic materials based on chitosan, alginate or fibrin with tricalcium phosphate].
24576958	0	10	with	chitosan	54:61	arg1	phosphate					99:107	tricalcium phosphate	88:107	tricalcium phosphate	88:107	[Comparative study of osteoplastic materials based on chitosan, alginate or fibrin with tricalcium phosphate].
24576958	0	11	theme	[Comparative	0:11	arg1	study					13:17	[Comparative study	0:17	[Comparative study of osteoplastic materials	0:43	[Comparative study of osteoplastic materials based on chitosan, alginate or fibrin with tricalcium phosphate].
24576958	2	12	theme	positive	351:358	arg1	response					371:378	the most effective positive biological response	332:378	the most effective positive biological response	332:378	Histological findings on Wistar rat condyles showed that fibrin-beta-TCP-based composite had the most effective positive biological response.
24576958	0	13	theme	tricalcium	88:97	arg1	phosphate					99:107	tricalcium phosphate	88:107	tricalcium phosphate	88:107	[Comparative study of osteoplastic materials based on chitosan, alginate or fibrin with tricalcium phosphate].
24576958	0	14	with	alginate	64:71	arg1	phosphate					99:107	tricalcium phosphate	88:107	tricalcium phosphate	88:107	[Comparative study of osteoplastic materials based on chitosan, alginate or fibrin with tricalcium phosphate].
24576958	0	15	theme	osteoplastic	22:33	arg1	materials					35:43	osteoplastic materials	22:43	osteoplastic materials	22:43	[Comparative study of osteoplastic materials based on chitosan, alginate or fibrin with tricalcium phosphate].
24576958	2	16	theme	rat	271:273	arg1	condyles					275:282	Wistar rat condyles	264:282	Wistar rat condyles	264:282	Histological findings on Wistar rat condyles showed that fibrin-beta-TCP-based composite had the most effective positive biological response.
24576958	2	17	theme	biological	360:369	arg1	response					371:378	the most effective positive biological response	332:378	the most effective positive biological response	332:378	Histological findings on Wistar rat condyles showed that fibrin-beta-TCP-based composite had the most effective positive biological response.
24576958	2	18	theme	Histological	239:250	arg1	findings					252:259	Histological findings	239:259	Histological findings on Wistar rat condyles	239:282	Histological findings on Wistar rat condyles showed that fibrin-beta-TCP-based composite had the most effective positive biological response.
24576958	2	19	contain	had	328:330	arg1	composite					318:326	fibrin-beta-TCP-based composite	296:326	fibrin-beta-TCP-based composite	296:326	Histological findings on Wistar rat condyles showed that fibrin-beta-TCP-based composite had the most effective positive biological response.
24576958	2	19	contain	had	328:330	arg2	response					371:378	the most effective positive biological response	332:378	the most effective positive biological response	332:378	Histological findings on Wistar rat condyles showed that fibrin-beta-TCP-based composite had the most effective positive biological response.
24576958	2	20	from	findings	252:259	arg1	condyles					275:282	Wistar rat condyles	264:282	Wistar rat condyles	264:282	Histological findings on Wistar rat condyles showed that fibrin-beta-TCP-based composite had the most effective positive biological response.
25843841	0	0	from	structure	19:27	arg1	relation					80:87	relation	80:87	relation to their biological activity	80:116	The supramolecular structure of LPS-chitosan complexes of varied composition in relation to their biological activity.
25843841	6	1	theme	stimulated	908:917	arg1	cells					937:941	stimulated human mononuclear cells	908:941	stimulated human mononuclear cells	908:941	The ability of LPS-Ch complex to induce biosynthesis of TNF-α and reactive oxygen species in stimulated human mononuclear cells was studied.
25843841	6	2	theme	reactive	881:888	arg1	species					897:903	reactive oxygen species	881:903	reactive oxygen species	881:903	The ability of LPS-Ch complex to induce biosynthesis of TNF-α and reactive oxygen species in stimulated human mononuclear cells was studied.
25843841	2	3	theme	negative	470:477	arg1	values					491:496	negative to positive values	470:496	negative to positive values	470:496	The alteration of ζ-potential of E-LPS-Ch complexes from negative to positive values depending on Ch content was detected.
25843841	2	4	from	ζ-potential	431:441	arg1	values					491:496	negative to positive values	470:496	negative to positive values	470:496	The alteration of ζ-potential of E-LPS-Ch complexes from negative to positive values depending on Ch content was detected.
25843841	0	5	theme	biological	98:107	arg1	activity					109:116	their biological activity	92:116	their biological activity	92:116	The supramolecular structure of LPS-chitosan complexes of varied composition in relation to their biological activity.
25843841	6	6	theme	oxygen	890:895	arg1	species					897:903	reactive oxygen species	881:903	reactive oxygen species	881:903	The ability of LPS-Ch complex to induce biosynthesis of TNF-α and reactive oxygen species in stimulated human mononuclear cells was studied.
25843841	7	7	theme	significant	958:968	arg1	decrease					970:977	A significant decrease	956:977	A significant decrease in activity complexes compared to that of the initial LPS	956:1035	A significant decrease in activity complexes compared to that of the initial LPS was observed only for E-LPS-Ch complexes.
25843841	1	8	theme	Yersinia	223:230	arg1	pseudotuberculosis					232:249	Yersinia pseudotuberculosis	223:249	Yersinia pseudotuberculosis	223:249	The complexes of chitosan (Ch) with lipopolysaccharides (LPSs) from Escherichia coli O55:B5 (E-LPS) and Yersinia pseudotuberculosis 1B 598 (Y-LPS) of various weight compositions were investigated using quasi-elastic light scattering, ζ-potential distribution assay and atomic force microscopy.
25843841	2	9	theme	Ch	511:512	arg1	content					514:520	Ch content	511:520	Ch content	511:520	The alteration of ζ-potential of E-LPS-Ch complexes from negative to positive values depending on Ch content was detected.
25843841	1	10	theme	quasi-elastic	321:333	arg1	scattering					341:350	quasi-elastic light scattering	321:350	quasi-elastic light scattering	321:350	The complexes of chitosan (Ch) with lipopolysaccharides (LPSs) from Escherichia coli O55:B5 (E-LPS) and Yersinia pseudotuberculosis 1B 598 (Y-LPS) of various weight compositions were investigated using quasi-elastic light scattering, ζ-potential distribution assay and atomic force microscopy.
25843841	6	11	theme	mononuclear	925:935	arg1	cells					937:941	stimulated human mononuclear cells	908:941	stimulated human mononuclear cells	908:941	The ability of LPS-Ch complex to induce biosynthesis of TNF-α and reactive oxygen species in stimulated human mononuclear cells was studied.
25843841	1	12	theme	light	335:339	arg1	scattering					341:350	quasi-elastic light scattering	321:350	quasi-elastic light scattering	321:350	The complexes of chitosan (Ch) with lipopolysaccharides (LPSs) from Escherichia coli O55:B5 (E-LPS) and Yersinia pseudotuberculosis 1B 598 (Y-LPS) of various weight compositions were investigated using quasi-elastic light scattering, ζ-potential distribution assay and atomic force microscopy.
25843841	7	13	from	decrease	970:977	arg1	complexes					991:999	activity complexes	982:999	activity complexes	982:999	A significant decrease in activity complexes compared to that of the initial LPS was observed only for E-LPS-Ch complexes.
25843841	5	14	theme	Y-LPS	741:745	arg1	particles					747:755	E-LPS and Y-LPS particles	731:755	E-LPS and Y-LPS particles	731:755	Screening of E-LPS and Y-LPS particles by Ch in the complexes with high polycation was detected.
25843841	6	15	theme	TNF-α	871:875	arg1	biosynthesis					855:866	biosynthesis	855:866	biosynthesis of TNF-α and reactive oxygen species in stimulated human mononuclear cells	855:941	The ability of LPS-Ch complex to induce biosynthesis of TNF-α and reactive oxygen species in stimulated human mononuclear cells was studied.
25843841	7	16	theme	E-LPS-Ch	1059:1066	arg1	complexes					1068:1076	E-LPS-Ch complexes	1059:1076	E-LPS-Ch complexes	1059:1076	A significant decrease in activity complexes compared to that of the initial LPS was observed only for E-LPS-Ch complexes.
25843841	5	17	with	complexes	770:778	arg1	polycation					790:799	high polycation	785:799	high polycation	785:799	Screening of E-LPS and Y-LPS particles by Ch in the complexes with high polycation was detected.
25843841	4	18	theme	structure	660:668	arg1	transformation					623:636	The transformation	619:636	The transformation of the supramolecular structure of E-LPS after binding with to Ch	619:702	The transformation of the supramolecular structure of E-LPS after binding with to Ch was revealed.
25843841	0	19	theme	supramolecular	4:17	arg1	structure					19:27	The supramolecular structure	0:27	The supramolecular structure of LPS-chitosan complexes of varied composition in relation to their biological activity.	0:117	The supramolecular structure of LPS-chitosan complexes of varied composition in relation to their biological activity.
25843841	2	20	dep	negative	470:477	arg1	positive					482:489	positive	482:489	positive	482:489	The alteration of ζ-potential of E-LPS-Ch complexes from negative to positive values depending on Ch content was detected.
25843841	2	20	dep	negative	470:477	arg1	to					479:480	to	479:480	to	479:480	The alteration of ζ-potential of E-LPS-Ch complexes from negative to positive values depending on Ch content was detected.
25843841	6	21	theme	species	897:903	arg1	biosynthesis					855:866	biosynthesis	855:866	biosynthesis of TNF-α and reactive oxygen species in stimulated human mononuclear cells	855:941	The ability of LPS-Ch complex to induce biosynthesis of TNF-α and reactive oxygen species in stimulated human mononuclear cells was studied.
25843841	1	22	theme	1B	251:252	arg1	598					254:256	1B 598	251:256	1B 598	251:256	The complexes of chitosan (Ch) with lipopolysaccharides (LPSs) from Escherichia coli O55:B5 (E-LPS) and Yersinia pseudotuberculosis 1B 598 (Y-LPS) of various weight compositions were investigated using quasi-elastic light scattering, ζ-potential distribution assay and atomic force microscopy.
25843841	1	23	with	complexes	123:131	arg1	LPSs					176:179	LPSs	176:179	LPSs	176:179	The complexes of chitosan (Ch) with lipopolysaccharides (LPSs) from Escherichia coli O55:B5 (E-LPS) and Yersinia pseudotuberculosis 1B 598 (Y-LPS) of various weight compositions were investigated using quasi-elastic light scattering, ζ-potential distribution assay and atomic force microscopy.
25843841	1	23	with	complexes	123:131	arg1	lipopolysaccharides					155:173	lipopolysaccharides	155:173	lipopolysaccharides (LPSs) from Escherichia coli O55:B5 (E-LPS) and Yersinia pseudotuberculosis 1B 598 (Y-LPS) of various weight compositions	155:295	The complexes of chitosan (Ch) with lipopolysaccharides (LPSs) from Escherichia coli O55:B5 (E-LPS) and Yersinia pseudotuberculosis 1B 598 (Y-LPS) of various weight compositions were investigated using quasi-elastic light scattering, ζ-potential distribution assay and atomic force microscopy.
25843841	3	24	theme	similar	563:569	arg1	ζ-potentials					580:591	similar positive ζ-potentials	563:591	similar positive ζ-potentials	563:591	The Y-LPS-Ch complexes had similar positive ζ-potentials regardless of Ch content.
25843841	4	25	theme	E-LPS	673:677	arg1	structure					660:668	the supramolecular structure	641:668	the supramolecular structure of E-LPS	641:677	The transformation of the supramolecular structure of E-LPS after binding with to Ch was revealed.
25843841	6	26	from	biosynthesis	855:866	arg1	cells					937:941	stimulated human mononuclear cells	908:941	stimulated human mononuclear cells	908:941	The ability of LPS-Ch complex to induce biosynthesis of TNF-α and reactive oxygen species in stimulated human mononuclear cells was studied.
25843841	3	27	theme	Y-LPS-Ch	540:547	arg1	complexes					549:557	The Y-LPS-Ch complexes	536:557	The Y-LPS-Ch complexes	536:557	The Y-LPS-Ch complexes had similar positive ζ-potentials regardless of Ch content.
25843841	6	28	theme	LPS-Ch	830:835	arg1	complex					837:843	LPS-Ch complex	830:843	LPS-Ch complex	830:843	The ability of LPS-Ch complex to induce biosynthesis of TNF-α and reactive oxygen species in stimulated human mononuclear cells was studied.
25843841	1	29	from	pseudotuberculosis	232:249	arg1	LPSs					176:179	LPSs	176:179	LPSs	176:179	The complexes of chitosan (Ch) with lipopolysaccharides (LPSs) from Escherichia coli O55:B5 (E-LPS) and Yersinia pseudotuberculosis 1B 598 (Y-LPS) of various weight compositions were investigated using quasi-elastic light scattering, ζ-potential distribution assay and atomic force microscopy.
25843841	1	29	from	pseudotuberculosis	232:249	arg1	lipopolysaccharides					155:173	lipopolysaccharides	155:173	lipopolysaccharides (LPSs) from Escherichia coli O55:B5 (E-LPS) and Yersinia pseudotuberculosis 1B 598 (Y-LPS) of various weight compositions	155:295	The complexes of chitosan (Ch) with lipopolysaccharides (LPSs) from Escherichia coli O55:B5 (E-LPS) and Yersinia pseudotuberculosis 1B 598 (Y-LPS) of various weight compositions were investigated using quasi-elastic light scattering, ζ-potential distribution assay and atomic force microscopy.
25843841	1	30	theme	ζ-potential	353:363	arg1	assay					378:382	ζ-potential distribution assay	353:382	ζ-potential distribution assay	353:382	The complexes of chitosan (Ch) with lipopolysaccharides (LPSs) from Escherichia coli O55:B5 (E-LPS) and Yersinia pseudotuberculosis 1B 598 (Y-LPS) of various weight compositions were investigated using quasi-elastic light scattering, ζ-potential distribution assay and atomic force microscopy.
25843841	2	31	theme	E-LPS-Ch	446:453	arg1	complexes					455:463	E-LPS-Ch complexes	446:463	E-LPS-Ch complexes from negative to positive values	446:496	The alteration of ζ-potential of E-LPS-Ch complexes from negative to positive values depending on Ch content was detected.
25843841	1	32	theme	Escherichia	187:197	arg1	Y-LPS					259:263	Y-LPS	259:263	Y-LPS	259:263	The complexes of chitosan (Ch) with lipopolysaccharides (LPSs) from Escherichia coli O55:B5 (E-LPS) and Yersinia pseudotuberculosis 1B 598 (Y-LPS) of various weight compositions were investigated using quasi-elastic light scattering, ζ-potential distribution assay and atomic force microscopy.
25843841	1	32	theme	Escherichia	187:197	arg1	E-LPS					212:216	E-LPS	212:216	E-LPS	212:216	The complexes of chitosan (Ch) with lipopolysaccharides (LPSs) from Escherichia coli O55:B5 (E-LPS) and Yersinia pseudotuberculosis 1B 598 (Y-LPS) of various weight compositions were investigated using quasi-elastic light scattering, ζ-potential distribution assay and atomic force microscopy.
25843841	1	32	theme	Escherichia	187:197	arg1	O55					204:206	Escherichia coli O55	187:206	Escherichia coli O55:B5 (E-LPS)	187:217	The complexes of chitosan (Ch) with lipopolysaccharides (LPSs) from Escherichia coli O55:B5 (E-LPS) and Yersinia pseudotuberculosis 1B 598 (Y-LPS) of various weight compositions were investigated using quasi-elastic light scattering, ζ-potential distribution assay and atomic force microscopy.
25843841	0	33	theme	complexes	45:53	arg1	structure					19:27	The supramolecular structure	0:27	The supramolecular structure of LPS-chitosan complexes of varied composition in relation to their biological activity.	0:117	The supramolecular structure of LPS-chitosan complexes of varied composition in relation to their biological activity.
25843841	3	34	contain	had	559:561	arg1	complexes					549:557	The Y-LPS-Ch complexes	536:557	The Y-LPS-Ch complexes	536:557	The Y-LPS-Ch complexes had similar positive ζ-potentials regardless of Ch content.
25843841	3	34	contain	had	559:561	arg2	ζ-potentials					580:591	similar positive ζ-potentials	563:591	similar positive ζ-potentials	563:591	The Y-LPS-Ch complexes had similar positive ζ-potentials regardless of Ch content.
25843841	1	35	theme	distribution	365:376	arg1	assay					378:382	ζ-potential distribution assay	353:382	ζ-potential distribution assay	353:382	The complexes of chitosan (Ch) with lipopolysaccharides (LPSs) from Escherichia coli O55:B5 (E-LPS) and Yersinia pseudotuberculosis 1B 598 (Y-LPS) of various weight compositions were investigated using quasi-elastic light scattering, ζ-potential distribution assay and atomic force microscopy.
25843841	4	36	theme	supramolecular	645:658	arg1	structure					660:668	the supramolecular structure	641:668	the supramolecular structure of E-LPS	641:677	The transformation of the supramolecular structure of E-LPS after binding with to Ch was revealed.
25843841	0	37	theme	LPS-chitosan	32:43	arg1	complexes					45:53	LPS-chitosan complexes	32:53	LPS-chitosan complexes of varied composition	32:75	The supramolecular structure of LPS-chitosan complexes of varied composition in relation to their biological activity.
25843841	2	38	theme	complexes	455:463	arg1	ζ-potential					431:441	ζ-potential	431:441	ζ-potential of E-LPS-Ch complexes from negative to positive values	431:496	The alteration of ζ-potential of E-LPS-Ch complexes from negative to positive values depending on Ch content was detected.
25843841	2	39	from	values	491:496	arg1	ζ-potential					431:441	ζ-potential	431:441	ζ-potential of E-LPS-Ch complexes from negative to positive values	431:496	The alteration of ζ-potential of E-LPS-Ch complexes from negative to positive values depending on Ch content was detected.
25843841	2	39	from	values	491:496	arg1	complexes					455:463	E-LPS-Ch complexes	446:463	E-LPS-Ch complexes from negative to positive values	446:496	The alteration of ζ-potential of E-LPS-Ch complexes from negative to positive values depending on Ch content was detected.
25843841	6	40	theme	human	919:923	arg1	cells					937:941	stimulated human mononuclear cells	908:941	stimulated human mononuclear cells	908:941	The ability of LPS-Ch complex to induce biosynthesis of TNF-α and reactive oxygen species in stimulated human mononuclear cells was studied.
25843841	0	41	theme	varied	58:63	arg1	composition					65:75	varied composition	58:75	varied composition	58:75	The supramolecular structure of LPS-chitosan complexes of varied composition in relation to their biological activity.
25843841	6	42	theme	complex	837:843	arg1	ability					819:825	The ability	815:825	The ability of LPS-Ch complex to induce biosynthesis of TNF-α and reactive oxygen species in stimulated human mononuclear cells	815:941	The ability of LPS-Ch complex to induce biosynthesis of TNF-α and reactive oxygen species in stimulated human mononuclear cells was studied.
25843841	3	43	theme	positive	571:578	arg1	ζ-potentials					580:591	similar positive ζ-potentials	563:591	similar positive ζ-potentials	563:591	The Y-LPS-Ch complexes had similar positive ζ-potentials regardless of Ch content.
25843841	1	44	from	O55	204:206	arg1	LPSs					176:179	LPSs	176:179	LPSs	176:179	The complexes of chitosan (Ch) with lipopolysaccharides (LPSs) from Escherichia coli O55:B5 (E-LPS) and Yersinia pseudotuberculosis 1B 598 (Y-LPS) of various weight compositions were investigated using quasi-elastic light scattering, ζ-potential distribution assay and atomic force microscopy.
25843841	1	44	from	O55	204:206	arg1	lipopolysaccharides					155:173	lipopolysaccharides	155:173	lipopolysaccharides (LPSs) from Escherichia coli O55:B5 (E-LPS) and Yersinia pseudotuberculosis 1B 598 (Y-LPS) of various weight compositions	155:295	The complexes of chitosan (Ch) with lipopolysaccharides (LPSs) from Escherichia coli O55:B5 (E-LPS) and Yersinia pseudotuberculosis 1B 598 (Y-LPS) of various weight compositions were investigated using quasi-elastic light scattering, ζ-potential distribution assay and atomic force microscopy.
25843841	5	45	theme	high	785:788	arg1	polycation					790:799	high polycation	785:799	high polycation	785:799	Screening of E-LPS and Y-LPS particles by Ch in the complexes with high polycation was detected.
25843841	1	46	theme	various	269:275	arg1	compositions					284:295	various weight compositions	269:295	various weight compositions	269:295	The complexes of chitosan (Ch) with lipopolysaccharides (LPSs) from Escherichia coli O55:B5 (E-LPS) and Yersinia pseudotuberculosis 1B 598 (Y-LPS) of various weight compositions were investigated using quasi-elastic light scattering, ζ-potential distribution assay and atomic force microscopy.
25843841	2	47	from	alteration	417:426	arg1	content					514:520	Ch content	511:520	Ch content	511:520	The alteration of ζ-potential of E-LPS-Ch complexes from negative to positive values depending on Ch content was detected.
25843841	1	48	theme	atomic	388:393	arg1	microscopy					401:410	atomic force microscopy	388:410	atomic force microscopy	388:410	The complexes of chitosan (Ch) with lipopolysaccharides (LPSs) from Escherichia coli O55:B5 (E-LPS) and Yersinia pseudotuberculosis 1B 598 (Y-LPS) of various weight compositions were investigated using quasi-elastic light scattering, ζ-potential distribution assay and atomic force microscopy.
25843841	5	49	from	Ch	760:761	arg1	complexes					770:778	the complexes	766:778	the complexes with high polycation	766:799	Screening of E-LPS and Y-LPS particles by Ch in the complexes with high polycation was detected.
25843841	1	50	theme	weight	277:282	arg1	compositions					284:295	various weight compositions	269:295	various weight compositions	269:295	The complexes of chitosan (Ch) with lipopolysaccharides (LPSs) from Escherichia coli O55:B5 (E-LPS) and Yersinia pseudotuberculosis 1B 598 (Y-LPS) of various weight compositions were investigated using quasi-elastic light scattering, ζ-potential distribution assay and atomic force microscopy.
25843841	1	51	theme	force	395:399	arg1	microscopy					401:410	atomic force microscopy	388:410	atomic force microscopy	388:410	The complexes of chitosan (Ch) with lipopolysaccharides (LPSs) from Escherichia coli O55:B5 (E-LPS) and Yersinia pseudotuberculosis 1B 598 (Y-LPS) of various weight compositions were investigated using quasi-elastic light scattering, ζ-potential distribution assay and atomic force microscopy.
25843841	7	52	theme	initial	1025:1031	arg1	LPS					1033:1035	the initial LPS	1021:1035	the initial LPS	1021:1035	A significant decrease in activity complexes compared to that of the initial LPS was observed only for E-LPS-Ch complexes.
25843841	1	53	dep	O55	204:206	arg1	B5					208:209	B5	208:209	B5	208:209	The complexes of chitosan (Ch) with lipopolysaccharides (LPSs) from Escherichia coli O55:B5 (E-LPS) and Yersinia pseudotuberculosis 1B 598 (Y-LPS) of various weight compositions were investigated using quasi-elastic light scattering, ζ-potential distribution assay and atomic force microscopy.
25843841	1	53	dep	O55	204:206	arg1	598					254:256	1B 598	251:256	1B 598	251:256	The complexes of chitosan (Ch) with lipopolysaccharides (LPSs) from Escherichia coli O55:B5 (E-LPS) and Yersinia pseudotuberculosis 1B 598 (Y-LPS) of various weight compositions were investigated using quasi-elastic light scattering, ζ-potential distribution assay and atomic force microscopy.
25843841	7	54	theme	activity	982:989	arg1	complexes					991:999	activity complexes	982:999	activity complexes	982:999	A significant decrease in activity complexes compared to that of the initial LPS was observed only for E-LPS-Ch complexes.
25843841	1	55	theme	chitosan	136:143	arg1	complexes					123:131	The complexes	119:131	The complexes of chitosan (Ch) with lipopolysaccharides (LPSs) from Escherichia coli O55:B5 (E-LPS) and Yersinia pseudotuberculosis 1B 598 (Y-LPS) of various weight compositions	119:295	The complexes of chitosan (Ch) with lipopolysaccharides (LPSs) from Escherichia coli O55:B5 (E-LPS) and Yersinia pseudotuberculosis 1B 598 (Y-LPS) of various weight compositions were investigated using quasi-elastic light scattering, ζ-potential distribution assay and atomic force microscopy.
25843841	5	56	theme	particles	747:755	arg1	Screening					718:726	Screening	718:726	Screening of E-LPS and Y-LPS particles by Ch in the complexes with high polycation	718:799	Screening of E-LPS and Y-LPS particles by Ch in the complexes with high polycation was detected.
25843841	3	57	theme	Ch	607:608	arg1	content					610:616	Ch content	607:616	Ch content	607:616	The Y-LPS-Ch complexes had similar positive ζ-potentials regardless of Ch content.
25843841	0	58	theme	composition	65:75	arg1	complexes					45:53	LPS-chitosan complexes	32:53	LPS-chitosan complexes of varied composition	32:75	The supramolecular structure of LPS-chitosan complexes of varied composition in relation to their biological activity.
25843841	1	59	theme	compositions	284:295	arg1	Y-LPS					259:263	Y-LPS	259:263	Y-LPS	259:263	The complexes of chitosan (Ch) with lipopolysaccharides (LPSs) from Escherichia coli O55:B5 (E-LPS) and Yersinia pseudotuberculosis 1B 598 (Y-LPS) of various weight compositions were investigated using quasi-elastic light scattering, ζ-potential distribution assay and atomic force microscopy.
25843841	1	59	theme	compositions	284:295	arg1	pseudotuberculosis					232:249	Yersinia pseudotuberculosis	223:249	Yersinia pseudotuberculosis	223:249	The complexes of chitosan (Ch) with lipopolysaccharides (LPSs) from Escherichia coli O55:B5 (E-LPS) and Yersinia pseudotuberculosis 1B 598 (Y-LPS) of various weight compositions were investigated using quasi-elastic light scattering, ζ-potential distribution assay and atomic force microscopy.
25843841	1	59	theme	compositions	284:295	arg1	E-LPS					212:216	E-LPS	212:216	E-LPS	212:216	The complexes of chitosan (Ch) with lipopolysaccharides (LPSs) from Escherichia coli O55:B5 (E-LPS) and Yersinia pseudotuberculosis 1B 598 (Y-LPS) of various weight compositions were investigated using quasi-elastic light scattering, ζ-potential distribution assay and atomic force microscopy.
25843841	1	59	theme	compositions	284:295	arg1	O55					204:206	Escherichia coli O55	187:206	Escherichia coli O55:B5 (E-LPS)	187:217	The complexes of chitosan (Ch) with lipopolysaccharides (LPSs) from Escherichia coli O55:B5 (E-LPS) and Yersinia pseudotuberculosis 1B 598 (Y-LPS) of various weight compositions were investigated using quasi-elastic light scattering, ζ-potential distribution assay and atomic force microscopy.
25843841	1	60	dep	Escherichia	187:197	arg1	coli					199:202	coli	199:202	coli	199:202	The complexes of chitosan (Ch) with lipopolysaccharides (LPSs) from Escherichia coli O55:B5 (E-LPS) and Yersinia pseudotuberculosis 1B 598 (Y-LPS) of various weight compositions were investigated using quasi-elastic light scattering, ζ-potential distribution assay and atomic force microscopy.
25843841	2	61	theme	ζ-potential	431:441	arg1	alteration					417:426	The alteration	413:426	The alteration of ζ-potential of E-LPS-Ch complexes from negative to positive values depending on Ch content	413:520	The alteration of ζ-potential of E-LPS-Ch complexes from negative to positive values depending on Ch content was detected.
25843841	5	62	theme	E-LPS	731:735	arg1	particles					747:755	E-LPS and Y-LPS particles	731:755	E-LPS and Y-LPS particles	731:755	Screening of E-LPS and Y-LPS particles by Ch in the complexes with high polycation was detected.
24770932	0	0	theme	junctions	80:88	arg1	constituent					37:47	a cytoplasmic plaque constituent	16:47	a cytoplasmic plaque constituent of various epithelial adherens junctions and composite junctions of myocardial intercalated disks: a unifying finding for cell biology and cardiology	16:197	Protein LUMA is a cytoplasmic plaque constituent of various epithelial adherens junctions and composite junctions of myocardial intercalated disks: a unifying finding for cell biology and cardiology.
24770932	0	0	theme	junctions	80:88	arg1	LUMA					8:11	Protein LUMA	0:11	Protein LUMA	0:11	Protein LUMA is a cytoplasmic plaque constituent of various epithelial adherens junctions and composite junctions of myocardial intercalated disks: a unifying finding for cell biology and cardiology.
24770932	7	1	theme	mutations	1477:1485	arg1	list					1469:1472	the list	1465:1472	the list of mutations of cardiomyocyte junction proteins that may be involved in cardiomyopathies	1465:1561	These findings now also allow the general conclusion that LUMA has to be added to the list of mutations of cardiomyocyte junction proteins that may be involved in cardiomyopathies.
24770932	1	2	theme	sudden	490:495	arg1	death					497:501	sudden death	490:501	sudden death	490:501	In a series of recent reports, mutations in the gene encoding a protein called LUMA (or TMEM43), widely speculated to be a tetraspan transmembrane protein of the nuclear envelope, have been associated with a specific subtype of cardiomyopathy (arrhythmogenic cardiomyopathies) and cases of sudden death.
24770932	2	3	theme	high	533:536	arg1	specificity					538:548	high specificity	533:548	high specificity	533:548	However, using antibodies of high specificity in immunolocalization experiments, we have discovered that, in mammals, LUMA is a component of zonula adhaerens and punctum adhaerens plaques of diverse epithelia and epithelial cell cultures and is also located in (or in some species associated with) the plaques of composite junctions (CJs) in myocardiac intercalated disks (IDs).
24770932	2	4	theme	composite	817:825	arg1	CJs					838:840	CJs	838:840	CJs	838:840	However, using antibodies of high specificity in immunolocalization experiments, we have discovered that, in mammals, LUMA is a component of zonula adhaerens and punctum adhaerens plaques of diverse epithelia and epithelial cell cultures and is also located in (or in some species associated with) the plaques of composite junctions (CJs) in myocardiac intercalated disks (IDs).
24770932	2	4	theme	composite	817:825	arg1	junctions					827:835	composite junctions	817:835	composite junctions (CJs)	817:841	However, using antibodies of high specificity in immunolocalization experiments, we have discovered that, in mammals, LUMA is a component of zonula adhaerens and punctum adhaerens plaques of diverse epithelia and epithelial cell cultures and is also located in (or in some species associated with) the plaques of composite junctions (CJs) in myocardiac intercalated disks (IDs).
24770932	0	5	theme	adherens	71:78	arg1	junctions					80:88	various epithelial adherens junctions	52:88	various epithelial adherens junctions	52:88	Protein LUMA is a cytoplasmic plaque constituent of various epithelial adherens junctions and composite junctions of myocardial intercalated disks: a unifying finding for cell biology and cardiology.
24770932	0	6	theme	cell	171:174	arg1	biology					176:182	cell biology	171:182	cell biology	171:182	Protein LUMA is a cytoplasmic plaque constituent of various epithelial adherens junctions and composite junctions of myocardial intercalated disks: a unifying finding for cell biology and cardiology.
24770932	2	7	theme	specificity	538:548	arg1	antibodies					519:528	antibodies	519:528	antibodies of high specificity	519:548	However, using antibodies of high specificity in immunolocalization experiments, we have discovered that, in mammals, LUMA is a component of zonula adhaerens and punctum adhaerens plaques of diverse epithelia and epithelial cell cultures and is also located in (or in some species associated with) the plaques of composite junctions (CJs) in myocardiac intercalated disks (IDs).
24770932	0	8	theme	composite	94:102	arg1	junctions					104:112	composite junctions	94:112	composite junctions of myocardial intercalated disks: a unifying finding for cell biology and cardiology	94:197	Protein LUMA is a cytoplasmic plaque constituent of various epithelial adherens junctions and composite junctions of myocardial intercalated disks: a unifying finding for cell biology and cardiology.
24770932	3	9	theme	several	919:925	arg1	proteins					943:950	several other CJ marker proteins	919:950	several other CJ marker proteins	919:950	In CJs, LUMA often colocalizes with several other CJ marker proteins.
24770932	7	10	theme	general	1417:1423	arg1	conclusion					1425:1434	the general conclusion that LUMA has to be added to the list of mutations of cardiomyocyte junction proteins that may be involved in cardiomyopathies	1413:1561	the general conclusion that LUMA has to be added to the list of mutations of cardiomyocyte junction proteins that may be involved in cardiomyopathies	1413:1561	These findings now also allow the general conclusion that LUMA has to be added to the list of mutations of cardiomyocyte junction proteins that may be involved in cardiomyopathies.
24770932	2	11	theme	zonula	645:650	arg1	adhaerens					652:660	zonula adhaerens	645:660	zonula adhaerens	645:660	However, using antibodies of high specificity in immunolocalization experiments, we have discovered that, in mammals, LUMA is a component of zonula adhaerens and punctum adhaerens plaques of diverse epithelia and epithelial cell cultures and is also located in (or in some species associated with) the plaques of composite junctions (CJs) in myocardiac intercalated disks (IDs).
24770932	7	12	theme	proteins	1513:1520	arg1	mutations					1477:1485	mutations	1477:1485	mutations of cardiomyocyte junction proteins that may be involved in cardiomyopathies	1477:1561	These findings now also allow the general conclusion that LUMA has to be added to the list of mutations of cardiomyocyte junction proteins that may be involved in cardiomyopathies.
24770932	1	13	theme	death	497:501	arg1	subtype					417:423	a specific subtype	406:423	a specific subtype of cardiomyopathy (arrhythmogenic cardiomyopathies)	406:475	In a series of recent reports, mutations in the gene encoding a protein called LUMA (or TMEM43), widely speculated to be a tetraspan transmembrane protein of the nuclear envelope, have been associated with a specific subtype of cardiomyopathy (arrhythmogenic cardiomyopathies) and cases of sudden death.
24770932	1	13	theme	death	497:501	arg1	cases					481:485	cases	481:485	cases of sudden death	481:501	In a series of recent reports, mutations in the gene encoding a protein called LUMA (or TMEM43), widely speculated to be a tetraspan transmembrane protein of the nuclear envelope, have been associated with a specific subtype of cardiomyopathy (arrhythmogenic cardiomyopathies) and cases of sudden death.
24770932	6	14	theme	myocardiac	1221:1230	arg1	CJs					1232:1234	myocardiac CJs	1221:1234	myocardiac CJs	1221:1234	The identification of LUMA as a plaque component of myocardiac CJs leads to reconsiderations of the molecular composition and architecture, the development, the functions, and the pathogenic states of CJs and IDs.
24770932	4	15	theme	nuclear	1007:1013	arg1	envelope					1015:1022	the nuclear envelope	1003:1022	the nuclear envelope	1003:1022	In all these cells, LUMA has not been detected in the nuclear envelope.
24770932	2	16	located	located	754:760	arg1	plaques					806:812	the plaques	802:812	(or in some species associated with) the plaques of composite junctions (CJs) in myocardiac intercalated disks (IDs)	765:880	However, using antibodies of high specificity in immunolocalization experiments, we have discovered that, in mammals, LUMA is a component of zonula adhaerens and punctum adhaerens plaques of diverse epithelia and epithelial cell cultures and is also located in (or in some species associated with) the plaques of composite junctions (CJs) in myocardiac intercalated disks (IDs).
24770932	2	16	located	located	754:760	arg2	LUMA					622:625	LUMA	622:625	LUMA	622:625	However, using antibodies of high specificity in immunolocalization experiments, we have discovered that, in mammals, LUMA is a component of zonula adhaerens and punctum adhaerens plaques of diverse epithelia and epithelial cell cultures and is also located in (or in some species associated with) the plaques of composite junctions (CJs) in myocardiac intercalated disks (IDs).
24770932	2	16	located	located	754:760	arg2	component					632:640	a component	630:640	a component of zonula adhaerens and punctum adhaerens plaques of diverse epithelia and epithelial cell cultures	630:740	However, using antibodies of high specificity in immunolocalization experiments, we have discovered that, in mammals, LUMA is a component of zonula adhaerens and punctum adhaerens plaques of diverse epithelia and epithelial cell cultures and is also located in (or in some species associated with) the plaques of composite junctions (CJs) in myocardiac intercalated disks (IDs).
24770932	5	17	theme	available	1144:1152	arg1	antibodies					1120:1129	some antibodies	1115:1129	some antibodies commercially available for some time	1115:1166	Surprisingly, under certain conditions, similar CJ localizations have also been seen with some antibodies commercially available for some time.
24770932	2	18	theme	epithelia	703:711	arg1	adhaerens					652:660	zonula adhaerens	645:660	zonula adhaerens	645:660	However, using antibodies of high specificity in immunolocalization experiments, we have discovered that, in mammals, LUMA is a component of zonula adhaerens and punctum adhaerens plaques of diverse epithelia and epithelial cell cultures and is also located in (or in some species associated with) the plaques of composite junctions (CJs) in myocardiac intercalated disks (IDs).
24770932	2	18	theme	epithelia	703:711	arg1	plaques					684:690	punctum adhaerens plaques	666:690	punctum adhaerens plaques	666:690	However, using antibodies of high specificity in immunolocalization experiments, we have discovered that, in mammals, LUMA is a component of zonula adhaerens and punctum adhaerens plaques of diverse epithelia and epithelial cell cultures and is also located in (or in some species associated with) the plaques of composite junctions (CJs) in myocardiac intercalated disks (IDs).
24770932	0	19	theme	junctions	104:112	arg1	constituent					37:47	a cytoplasmic plaque constituent	16:47	a cytoplasmic plaque constituent of various epithelial adherens junctions and composite junctions of myocardial intercalated disks: a unifying finding for cell biology and cardiology	16:197	Protein LUMA is a cytoplasmic plaque constituent of various epithelial adherens junctions and composite junctions of myocardial intercalated disks: a unifying finding for cell biology and cardiology.
24770932	0	19	theme	junctions	104:112	arg1	LUMA					8:11	Protein LUMA	0:11	Protein LUMA	0:11	Protein LUMA is a cytoplasmic plaque constituent of various epithelial adherens junctions and composite junctions of myocardial intercalated disks: a unifying finding for cell biology and cardiology.
24770932	6	20	theme	CJs	1232:1234	arg1	component					1208:1216	a plaque component	1199:1216	a plaque component of myocardiac CJs	1199:1234	The identification of LUMA as a plaque component of myocardiac CJs leads to reconsiderations of the molecular composition and architecture, the development, the functions, and the pathogenic states of CJs and IDs.
24770932	1	21	theme	specific	408:415	arg1	subtype					417:423	a specific subtype	406:423	a specific subtype of cardiomyopathy (arrhythmogenic cardiomyopathies)	406:475	In a series of recent reports, mutations in the gene encoding a protein called LUMA (or TMEM43), widely speculated to be a tetraspan transmembrane protein of the nuclear envelope, have been associated with a specific subtype of cardiomyopathy (arrhythmogenic cardiomyopathies) and cases of sudden death.
24770932	2	22	from	located	754:760	arg1	plaques					806:812	the plaques	802:812	(or in some species associated with) the plaques of composite junctions (CJs) in myocardiac intercalated disks (IDs)	765:880	However, using antibodies of high specificity in immunolocalization experiments, we have discovered that, in mammals, LUMA is a component of zonula adhaerens and punctum adhaerens plaques of diverse epithelia and epithelial cell cultures and is also located in (or in some species associated with) the plaques of composite junctions (CJs) in myocardiac intercalated disks (IDs).
24770932	2	23	theme	diverse	695:701	arg1	epithelia					703:711	diverse epithelia	695:711	diverse epithelia	695:711	However, using antibodies of high specificity in immunolocalization experiments, we have discovered that, in mammals, LUMA is a component of zonula adhaerens and punctum adhaerens plaques of diverse epithelia and epithelial cell cultures and is also located in (or in some species associated with) the plaques of composite junctions (CJs) in myocardiac intercalated disks (IDs).
24770932	2	24	from	component	632:640	arg1	mammals					613:619	mammals	613:619	mammals	613:619	However, using antibodies of high specificity in immunolocalization experiments, we have discovered that, in mammals, LUMA is a component of zonula adhaerens and punctum adhaerens plaques of diverse epithelia and epithelial cell cultures and is also located in (or in some species associated with) the plaques of composite junctions (CJs) in myocardiac intercalated disks (IDs).
24770932	1	25	theme	tetraspan	323:331	arg1	protein					347:353	a tetraspan transmembrane protein	321:353	a tetraspan transmembrane protein of the nuclear envelope	321:377	In a series of recent reports, mutations in the gene encoding a protein called LUMA (or TMEM43), widely speculated to be a tetraspan transmembrane protein of the nuclear envelope, have been associated with a specific subtype of cardiomyopathy (arrhythmogenic cardiomyopathies) and cases of sudden death.
24770932	6	26	theme	composition	1279:1289	arg1	reconsiderations					1245:1260	reconsiderations	1245:1260	reconsiderations of the molecular composition and architecture	1245:1306	The identification of LUMA as a plaque component of myocardiac CJs leads to reconsiderations of the molecular composition and architecture, the development, the functions, and the pathogenic states of CJs and IDs.
24770932	6	26	theme	composition	1279:1289	arg1	development					1313:1323	the development	1309:1323	the development	1309:1323	The identification of LUMA as a plaque component of myocardiac CJs leads to reconsiderations of the molecular composition and architecture, the development, the functions, and the pathogenic states of CJs and IDs.
24770932	6	26	theme	composition	1279:1289	arg1	states					1360:1365	the pathogenic states	1345:1365	the pathogenic states of CJs and IDs	1345:1380	The identification of LUMA as a plaque component of myocardiac CJs leads to reconsiderations of the molecular composition and architecture, the development, the functions, and the pathogenic states of CJs and IDs.
24770932	6	26	theme	composition	1279:1289	arg1	functions					1330:1338	the functions	1326:1338	the functions	1326:1338	The identification of LUMA as a plaque component of myocardiac CJs leads to reconsiderations of the molecular composition and architecture, the development, the functions, and the pathogenic states of CJs and IDs.
24770932	5	27	theme	certain	1045:1051	arg1	conditions					1053:1062	certain conditions	1045:1062	certain conditions	1045:1062	Surprisingly, under certain conditions, similar CJ localizations have also been seen with some antibodies commercially available for some time.
24770932	0	28	theme	Protein	0:6	arg1	constituent					37:47	a cytoplasmic plaque constituent	16:47	a cytoplasmic plaque constituent of various epithelial adherens junctions and composite junctions of myocardial intercalated disks: a unifying finding for cell biology and cardiology	16:197	Protein LUMA is a cytoplasmic plaque constituent of various epithelial adherens junctions and composite junctions of myocardial intercalated disks: a unifying finding for cell biology and cardiology.
24770932	0	28	theme	Protein	0:6	arg1	LUMA					8:11	Protein LUMA	0:11	Protein LUMA	0:11	Protein LUMA is a cytoplasmic plaque constituent of various epithelial adherens junctions and composite junctions of myocardial intercalated disks: a unifying finding for cell biology and cardiology.
24770932	7	29	theme	cardiomyocyte	1490:1502	arg1	proteins					1513:1520	cardiomyocyte junction proteins	1490:1520	cardiomyocyte junction proteins that may be involved in cardiomyopathies	1490:1561	These findings now also allow the general conclusion that LUMA has to be added to the list of mutations of cardiomyocyte junction proteins that may be involved in cardiomyopathies.
24770932	3	30	theme	other	927:931	arg1	proteins					943:950	several other CJ marker proteins	919:950	several other CJ marker proteins	919:950	In CJs, LUMA often colocalizes with several other CJ marker proteins.
24770932	2	31	theme	immunolocalization	553:570	arg1	experiments					572:582	immunolocalization experiments	553:582	immunolocalization experiments	553:582	However, using antibodies of high specificity in immunolocalization experiments, we have discovered that, in mammals, LUMA is a component of zonula adhaerens and punctum adhaerens plaques of diverse epithelia and epithelial cell cultures and is also located in (or in some species associated with) the plaques of composite junctions (CJs) in myocardiac intercalated disks (IDs).
24770932	6	32	theme	LUMA	1191:1194	arg1	identification					1173:1186	The identification	1169:1186	The identification of LUMA as a plaque component of myocardiac CJs	1169:1234	The identification of LUMA as a plaque component of myocardiac CJs leads to reconsiderations of the molecular composition and architecture, the development, the functions, and the pathogenic states of CJs and IDs.
24770932	0	33	theme	intercalated	128:139	arg1	disks					141:145	myocardial intercalated disks	117:145	myocardial intercalated disks: a unifying finding for cell biology and cardiology	117:197	Protein LUMA is a cytoplasmic plaque constituent of various epithelial adherens junctions and composite junctions of myocardial intercalated disks: a unifying finding for cell biology and cardiology.
24770932	1	34	theme	cardiomyopathy	428:441	arg1	subtype					417:423	a specific subtype	406:423	a specific subtype of cardiomyopathy (arrhythmogenic cardiomyopathies)	406:475	In a series of recent reports, mutations in the gene encoding a protein called LUMA (or TMEM43), widely speculated to be a tetraspan transmembrane protein of the nuclear envelope, have been associated with a specific subtype of cardiomyopathy (arrhythmogenic cardiomyopathies) and cases of sudden death.
24770932	1	34	theme	cardiomyopathy	428:441	arg1	cases					481:485	cases	481:485	cases of sudden death	481:501	In a series of recent reports, mutations in the gene encoding a protein called LUMA (or TMEM43), widely speculated to be a tetraspan transmembrane protein of the nuclear envelope, have been associated with a specific subtype of cardiomyopathy (arrhythmogenic cardiomyopathies) and cases of sudden death.
24770932	2	35	theme	cultures	733:740	arg1	adhaerens					652:660	zonula adhaerens	645:660	zonula adhaerens	645:660	However, using antibodies of high specificity in immunolocalization experiments, we have discovered that, in mammals, LUMA is a component of zonula adhaerens and punctum adhaerens plaques of diverse epithelia and epithelial cell cultures and is also located in (or in some species associated with) the plaques of composite junctions (CJs) in myocardiac intercalated disks (IDs).
24770932	2	35	theme	cultures	733:740	arg1	plaques					684:690	punctum adhaerens plaques	666:690	punctum adhaerens plaques	666:690	However, using antibodies of high specificity in immunolocalization experiments, we have discovered that, in mammals, LUMA is a component of zonula adhaerens and punctum adhaerens plaques of diverse epithelia and epithelial cell cultures and is also located in (or in some species associated with) the plaques of composite junctions (CJs) in myocardiac intercalated disks (IDs).
24770932	7	36	theme	junction	1504:1511	arg1	proteins					1513:1520	cardiomyocyte junction proteins	1490:1520	cardiomyocyte junction proteins that may be involved in cardiomyopathies	1490:1561	These findings now also allow the general conclusion that LUMA has to be added to the list of mutations of cardiomyocyte junction proteins that may be involved in cardiomyopathies.
24770932	5	37	theme	similar	1065:1071	arg1	localizations					1076:1088	similar CJ localizations	1065:1088	similar CJ localizations	1065:1088	Surprisingly, under certain conditions, similar CJ localizations have also been seen with some antibodies commercially available for some time.
24770932	0	38	theme	myocardial	117:126	arg1	disks					141:145	myocardial intercalated disks	117:145	myocardial intercalated disks: a unifying finding for cell biology and cardiology	117:197	Protein LUMA is a cytoplasmic plaque constituent of various epithelial adherens junctions and composite junctions of myocardial intercalated disks: a unifying finding for cell biology and cardiology.
24770932	1	39	theme	transmembrane	333:345	arg1	protein					347:353	a tetraspan transmembrane protein	321:353	a tetraspan transmembrane protein of the nuclear envelope	321:377	In a series of recent reports, mutations in the gene encoding a protein called LUMA (or TMEM43), widely speculated to be a tetraspan transmembrane protein of the nuclear envelope, have been associated with a specific subtype of cardiomyopathy (arrhythmogenic cardiomyopathies) and cases of sudden death.
24770932	3	40	theme	marker	936:941	arg1	proteins					943:950	several other CJ marker proteins	919:950	several other CJ marker proteins	919:950	In CJs, LUMA often colocalizes with several other CJ marker proteins.
24770932	2	41	theme	cell	728:731	arg1	cultures					733:740	epithelial cell cultures	717:740	epithelial cell cultures	717:740	However, using antibodies of high specificity in immunolocalization experiments, we have discovered that, in mammals, LUMA is a component of zonula adhaerens and punctum adhaerens plaques of diverse epithelia and epithelial cell cultures and is also located in (or in some species associated with) the plaques of composite junctions (CJs) in myocardiac intercalated disks (IDs).
24770932	0	42	theme	plaque	30:35	arg1	constituent					37:47	a cytoplasmic plaque constituent	16:47	a cytoplasmic plaque constituent of various epithelial adherens junctions and composite junctions of myocardial intercalated disks: a unifying finding for cell biology and cardiology	16:197	Protein LUMA is a cytoplasmic plaque constituent of various epithelial adherens junctions and composite junctions of myocardial intercalated disks: a unifying finding for cell biology and cardiology.
24770932	0	42	theme	plaque	30:35	arg1	LUMA					8:11	Protein LUMA	0:11	Protein LUMA	0:11	Protein LUMA is a cytoplasmic plaque constituent of various epithelial adherens junctions and composite junctions of myocardial intercalated disks: a unifying finding for cell biology and cardiology.
24770932	2	43	theme	junctions	827:835	arg1	plaques					806:812	the plaques	802:812	(or in some species associated with) the plaques of composite junctions (CJs) in myocardiac intercalated disks (IDs)	765:880	However, using antibodies of high specificity in immunolocalization experiments, we have discovered that, in mammals, LUMA is a component of zonula adhaerens and punctum adhaerens plaques of diverse epithelia and epithelial cell cultures and is also located in (or in some species associated with) the plaques of composite junctions (CJs) in myocardiac intercalated disks (IDs).
24770932	1	44	from	mutations	231:239	arg1	gene					248:251	the gene	244:251	the gene encoding a protein called LUMA (or TMEM43), widely speculated to be a tetraspan transmembrane protein of the nuclear envelope,	244:378	In a series of recent reports, mutations in the gene encoding a protein called LUMA (or TMEM43), widely speculated to be a tetraspan transmembrane protein of the nuclear envelope, have been associated with a specific subtype of cardiomyopathy (arrhythmogenic cardiomyopathies) and cases of sudden death.
24770932	2	45	theme	epithelial	717:726	arg1	cultures					733:740	epithelial cell cultures	717:740	epithelial cell cultures	717:740	However, using antibodies of high specificity in immunolocalization experiments, we have discovered that, in mammals, LUMA is a component of zonula adhaerens and punctum adhaerens plaques of diverse epithelia and epithelial cell cultures and is also located in (or in some species associated with) the plaques of composite junctions (CJs) in myocardiac intercalated disks (IDs).
24770932	0	46	theme	cytoplasmic	18:28	arg1	constituent					37:47	a cytoplasmic plaque constituent	16:47	a cytoplasmic plaque constituent of various epithelial adherens junctions and composite junctions of myocardial intercalated disks: a unifying finding for cell biology and cardiology	16:197	Protein LUMA is a cytoplasmic plaque constituent of various epithelial adherens junctions and composite junctions of myocardial intercalated disks: a unifying finding for cell biology and cardiology.
24770932	0	46	theme	cytoplasmic	18:28	arg1	LUMA					8:11	Protein LUMA	0:11	Protein LUMA	0:11	Protein LUMA is a cytoplasmic plaque constituent of various epithelial adherens junctions and composite junctions of myocardial intercalated disks: a unifying finding for cell biology and cardiology.
24770932	6	47	theme	IDs	1378:1380	arg1	reconsiderations					1245:1260	reconsiderations	1245:1260	reconsiderations of the molecular composition and architecture	1245:1306	The identification of LUMA as a plaque component of myocardiac CJs leads to reconsiderations of the molecular composition and architecture, the development, the functions, and the pathogenic states of CJs and IDs.
24770932	6	47	theme	IDs	1378:1380	arg1	development					1313:1323	the development	1309:1323	the development	1309:1323	The identification of LUMA as a plaque component of myocardiac CJs leads to reconsiderations of the molecular composition and architecture, the development, the functions, and the pathogenic states of CJs and IDs.
24770932	6	47	theme	IDs	1378:1380	arg1	states					1360:1365	the pathogenic states	1345:1365	the pathogenic states of CJs and IDs	1345:1380	The identification of LUMA as a plaque component of myocardiac CJs leads to reconsiderations of the molecular composition and architecture, the development, the functions, and the pathogenic states of CJs and IDs.
24770932	6	47	theme	IDs	1378:1380	arg1	functions					1330:1338	the functions	1326:1338	the functions	1326:1338	The identification of LUMA as a plaque component of myocardiac CJs leads to reconsiderations of the molecular composition and architecture, the development, the functions, and the pathogenic states of CJs and IDs.
24770932	0	48	theme	disks	141:145	arg1	junctions					80:88	various epithelial adherens junctions	52:88	various epithelial adherens junctions	52:88	Protein LUMA is a cytoplasmic plaque constituent of various epithelial adherens junctions and composite junctions of myocardial intercalated disks: a unifying finding for cell biology and cardiology.
24770932	0	48	theme	disks	141:145	arg1	junctions					104:112	composite junctions	94:112	composite junctions of myocardial intercalated disks: a unifying finding for cell biology and cardiology	94:197	Protein LUMA is a cytoplasmic plaque constituent of various epithelial adherens junctions and composite junctions of myocardial intercalated disks: a unifying finding for cell biology and cardiology.
24770932	2	49	theme	adhaerens	652:660	arg1	LUMA					622:625	LUMA	622:625	LUMA	622:625	However, using antibodies of high specificity in immunolocalization experiments, we have discovered that, in mammals, LUMA is a component of zonula adhaerens and punctum adhaerens plaques of diverse epithelia and epithelial cell cultures and is also located in (or in some species associated with) the plaques of composite junctions (CJs) in myocardiac intercalated disks (IDs).
24770932	2	49	theme	adhaerens	652:660	arg1	component					632:640	a component	630:640	a component of zonula adhaerens and punctum adhaerens plaques of diverse epithelia and epithelial cell cultures	630:740	However, using antibodies of high specificity in immunolocalization experiments, we have discovered that, in mammals, LUMA is a component of zonula adhaerens and punctum adhaerens plaques of diverse epithelia and epithelial cell cultures and is also located in (or in some species associated with) the plaques of composite junctions (CJs) in myocardiac intercalated disks (IDs).
24770932	5	50	theme	CJ	1073:1074	arg1	localizations					1076:1088	similar CJ localizations	1065:1088	similar CJ localizations	1065:1088	Surprisingly, under certain conditions, similar CJ localizations have also been seen with some antibodies commercially available for some time.
24770932	6	51	theme	architecture	1295:1306	arg1	reconsiderations					1245:1260	reconsiderations	1245:1260	reconsiderations of the molecular composition and architecture	1245:1306	The identification of LUMA as a plaque component of myocardiac CJs leads to reconsiderations of the molecular composition and architecture, the development, the functions, and the pathogenic states of CJs and IDs.
24770932	6	51	theme	architecture	1295:1306	arg1	development					1313:1323	the development	1309:1323	the development	1309:1323	The identification of LUMA as a plaque component of myocardiac CJs leads to reconsiderations of the molecular composition and architecture, the development, the functions, and the pathogenic states of CJs and IDs.
24770932	6	51	theme	architecture	1295:1306	arg1	states					1360:1365	the pathogenic states	1345:1365	the pathogenic states of CJs and IDs	1345:1380	The identification of LUMA as a plaque component of myocardiac CJs leads to reconsiderations of the molecular composition and architecture, the development, the functions, and the pathogenic states of CJs and IDs.
24770932	6	51	theme	architecture	1295:1306	arg1	functions					1330:1338	the functions	1326:1338	the functions	1326:1338	The identification of LUMA as a plaque component of myocardiac CJs leads to reconsiderations of the molecular composition and architecture, the development, the functions, and the pathogenic states of CJs and IDs.
24770932	2	52	theme	intercalated	857:868	arg1	IDs					877:879	IDs	877:879	IDs	877:879	However, using antibodies of high specificity in immunolocalization experiments, we have discovered that, in mammals, LUMA is a component of zonula adhaerens and punctum adhaerens plaques of diverse epithelia and epithelial cell cultures and is also located in (or in some species associated with) the plaques of composite junctions (CJs) in myocardiac intercalated disks (IDs).
24770932	2	52	theme	intercalated	857:868	arg1	disks					870:874	myocardiac intercalated disks	846:874	myocardiac intercalated disks (IDs)	846:880	However, using antibodies of high specificity in immunolocalization experiments, we have discovered that, in mammals, LUMA is a component of zonula adhaerens and punctum adhaerens plaques of diverse epithelia and epithelial cell cultures and is also located in (or in some species associated with) the plaques of composite junctions (CJs) in myocardiac intercalated disks (IDs).
24770932	0	53	theme	unifying	150:157	arg1	finding					159:165	a unifying finding	148:165	myocardial intercalated disks: a unifying finding for cell biology and cardiology	117:197	Protein LUMA is a cytoplasmic plaque constituent of various epithelial adherens junctions and composite junctions of myocardial intercalated disks: a unifying finding for cell biology and cardiology.
24770932	6	54	theme	molecular	1269:1277	arg1	composition					1279:1289	the molecular composition	1265:1289	the molecular composition	1265:1289	The identification of LUMA as a plaque component of myocardiac CJs leads to reconsiderations of the molecular composition and architecture, the development, the functions, and the pathogenic states of CJs and IDs.
24770932	1	55	theme	arrhythmogenic	444:457	arg1	cardiomyopathies					459:474	arrhythmogenic cardiomyopathies	444:474	arrhythmogenic cardiomyopathies	444:474	In a series of recent reports, mutations in the gene encoding a protein called LUMA (or TMEM43), widely speculated to be a tetraspan transmembrane protein of the nuclear envelope, have been associated with a specific subtype of cardiomyopathy (arrhythmogenic cardiomyopathies) and cases of sudden death.
24770932	1	55	theme	arrhythmogenic	444:457	arg1	cardiomyopathy					428:441	cardiomyopathy	428:441	cardiomyopathy (arrhythmogenic cardiomyopathies)	428:475	In a series of recent reports, mutations in the gene encoding a protein called LUMA (or TMEM43), widely speculated to be a tetraspan transmembrane protein of the nuclear envelope, have been associated with a specific subtype of cardiomyopathy (arrhythmogenic cardiomyopathies) and cases of sudden death.
24770932	6	56	theme	plaque	1201:1206	arg1	component					1208:1216	a plaque component	1199:1216	a plaque component of myocardiac CJs	1199:1234	The identification of LUMA as a plaque component of myocardiac CJs leads to reconsiderations of the molecular composition and architecture, the development, the functions, and the pathogenic states of CJs and IDs.
24770932	2	57	theme	myocardiac	846:855	arg1	IDs					877:879	IDs	877:879	IDs	877:879	However, using antibodies of high specificity in immunolocalization experiments, we have discovered that, in mammals, LUMA is a component of zonula adhaerens and punctum adhaerens plaques of diverse epithelia and epithelial cell cultures and is also located in (or in some species associated with) the plaques of composite junctions (CJs) in myocardiac intercalated disks (IDs).
24770932	2	57	theme	myocardiac	846:855	arg1	disks					870:874	myocardiac intercalated disks	846:874	myocardiac intercalated disks (IDs)	846:880	However, using antibodies of high specificity in immunolocalization experiments, we have discovered that, in mammals, LUMA is a component of zonula adhaerens and punctum adhaerens plaques of diverse epithelia and epithelial cell cultures and is also located in (or in some species associated with) the plaques of composite junctions (CJs) in myocardiac intercalated disks (IDs).
24770932	2	58	from	plaques	806:812	arg1	located					754:760	located	754:760	located	754:760	However, using antibodies of high specificity in immunolocalization experiments, we have discovered that, in mammals, LUMA is a component of zonula adhaerens and punctum adhaerens plaques of diverse epithelia and epithelial cell cultures and is also located in (or in some species associated with) the plaques of composite junctions (CJs) in myocardiac intercalated disks (IDs).
24770932	2	58	from	plaques	806:812	arg1	species					777:783	some species	772:783	some species associated with	772:799	However, using antibodies of high specificity in immunolocalization experiments, we have discovered that, in mammals, LUMA is a component of zonula adhaerens and punctum adhaerens plaques of diverse epithelia and epithelial cell cultures and is also located in (or in some species associated with) the plaques of composite junctions (CJs) in myocardiac intercalated disks (IDs).
24770932	2	58	from	plaques	806:812	arg1	IDs					877:879	IDs	877:879	IDs	877:879	However, using antibodies of high specificity in immunolocalization experiments, we have discovered that, in mammals, LUMA is a component of zonula adhaerens and punctum adhaerens plaques of diverse epithelia and epithelial cell cultures and is also located in (or in some species associated with) the plaques of composite junctions (CJs) in myocardiac intercalated disks (IDs).
24770932	2	58	from	plaques	806:812	arg1	disks					870:874	myocardiac intercalated disks	846:874	myocardiac intercalated disks (IDs)	846:880	However, using antibodies of high specificity in immunolocalization experiments, we have discovered that, in mammals, LUMA is a component of zonula adhaerens and punctum adhaerens plaques of diverse epithelia and epithelial cell cultures and is also located in (or in some species associated with) the plaques of composite junctions (CJs) in myocardiac intercalated disks (IDs).
24770932	1	59	theme	nuclear	362:368	arg1	envelope					370:377	the nuclear envelope	358:377	the nuclear envelope	358:377	In a series of recent reports, mutations in the gene encoding a protein called LUMA (or TMEM43), widely speculated to be a tetraspan transmembrane protein of the nuclear envelope, have been associated with a specific subtype of cardiomyopathy (arrhythmogenic cardiomyopathies) and cases of sudden death.
24770932	3	60	theme	CJ	933:934	arg1	proteins					943:950	several other CJ marker proteins	919:950	several other CJ marker proteins	919:950	In CJs, LUMA often colocalizes with several other CJ marker proteins.
24770932	4	61	located	detected	991:998	arg2	LUMA					973:976	LUMA	973:976	LUMA	973:976	In all these cells, LUMA has not been detected in the nuclear envelope.
24770932	4	61	located	detected	991:998	arg1	envelope					1015:1022	the nuclear envelope	1003:1022	the nuclear envelope	1003:1022	In all these cells, LUMA has not been detected in the nuclear envelope.
24770932	4	61	located	detected	991:998	arg1	cells					966:970	all these cells	956:970	all these cells	956:970	In all these cells, LUMA has not been detected in the nuclear envelope.
24770932	1	62	theme	envelope	370:377	arg1	protein					347:353	a tetraspan transmembrane protein	321:353	a tetraspan transmembrane protein of the nuclear envelope	321:377	In a series of recent reports, mutations in the gene encoding a protein called LUMA (or TMEM43), widely speculated to be a tetraspan transmembrane protein of the nuclear envelope, have been associated with a specific subtype of cardiomyopathy (arrhythmogenic cardiomyopathies) and cases of sudden death.
24770932	6	63	theme	CJs	1370:1372	arg1	reconsiderations					1245:1260	reconsiderations	1245:1260	reconsiderations of the molecular composition and architecture	1245:1306	The identification of LUMA as a plaque component of myocardiac CJs leads to reconsiderations of the molecular composition and architecture, the development, the functions, and the pathogenic states of CJs and IDs.
24770932	6	63	theme	CJs	1370:1372	arg1	development					1313:1323	the development	1309:1323	the development	1309:1323	The identification of LUMA as a plaque component of myocardiac CJs leads to reconsiderations of the molecular composition and architecture, the development, the functions, and the pathogenic states of CJs and IDs.
24770932	6	63	theme	CJs	1370:1372	arg1	states					1360:1365	the pathogenic states	1345:1365	the pathogenic states of CJs and IDs	1345:1380	The identification of LUMA as a plaque component of myocardiac CJs leads to reconsiderations of the molecular composition and architecture, the development, the functions, and the pathogenic states of CJs and IDs.
24770932	6	63	theme	CJs	1370:1372	arg1	functions					1330:1338	the functions	1326:1338	the functions	1326:1338	The identification of LUMA as a plaque component of myocardiac CJs leads to reconsiderations of the molecular composition and architecture, the development, the functions, and the pathogenic states of CJs and IDs.
24770932	2	64	theme	plaques	684:690	arg1	LUMA					622:625	LUMA	622:625	LUMA	622:625	However, using antibodies of high specificity in immunolocalization experiments, we have discovered that, in mammals, LUMA is a component of zonula adhaerens and punctum adhaerens plaques of diverse epithelia and epithelial cell cultures and is also located in (or in some species associated with) the plaques of composite junctions (CJs) in myocardiac intercalated disks (IDs).
24770932	2	64	theme	plaques	684:690	arg1	component					632:640	a component	630:640	a component of zonula adhaerens and punctum adhaerens plaques of diverse epithelia and epithelial cell cultures	630:740	However, using antibodies of high specificity in immunolocalization experiments, we have discovered that, in mammals, LUMA is a component of zonula adhaerens and punctum adhaerens plaques of diverse epithelia and epithelial cell cultures and is also located in (or in some species associated with) the plaques of composite junctions (CJs) in myocardiac intercalated disks (IDs).
24770932	1	65	theme	recent	215:220	arg1	reports					222:228	recent reports	215:228	recent reports	215:228	In a series of recent reports, mutations in the gene encoding a protein called LUMA (or TMEM43), widely speculated to be a tetraspan transmembrane protein of the nuclear envelope, have been associated with a specific subtype of cardiomyopathy (arrhythmogenic cardiomyopathies) and cases of sudden death.
24770932	0	66	theme	epithelial	60:69	arg1	junctions					80:88	various epithelial adherens junctions	52:88	various epithelial adherens junctions	52:88	Protein LUMA is a cytoplasmic plaque constituent of various epithelial adherens junctions and composite junctions of myocardial intercalated disks: a unifying finding for cell biology and cardiology.
24770932	2	67	theme	adhaerens	674:682	arg1	plaques					684:690	punctum adhaerens plaques	666:690	punctum adhaerens plaques	666:690	However, using antibodies of high specificity in immunolocalization experiments, we have discovered that, in mammals, LUMA is a component of zonula adhaerens and punctum adhaerens plaques of diverse epithelia and epithelial cell cultures and is also located in (or in some species associated with) the plaques of composite junctions (CJs) in myocardiac intercalated disks (IDs).
24770932	1	68	theme	reports	222:228	arg1	series					205:210	a series	203:210	a series of recent reports	203:228	In a series of recent reports, mutations in the gene encoding a protein called LUMA (or TMEM43), widely speculated to be a tetraspan transmembrane protein of the nuclear envelope, have been associated with a specific subtype of cardiomyopathy (arrhythmogenic cardiomyopathies) and cases of sudden death.
24770932	0	69	theme	various	52:58	arg1	junctions					80:88	various epithelial adherens junctions	52:88	various epithelial adherens junctions	52:88	Protein LUMA is a cytoplasmic plaque constituent of various epithelial adherens junctions and composite junctions of myocardial intercalated disks: a unifying finding for cell biology and cardiology.
24770932	2	70	theme	punctum	666:672	arg1	plaques					684:690	punctum adhaerens plaques	666:690	punctum adhaerens plaques	666:690	However, using antibodies of high specificity in immunolocalization experiments, we have discovered that, in mammals, LUMA is a component of zonula adhaerens and punctum adhaerens plaques of diverse epithelia and epithelial cell cultures and is also located in (or in some species associated with) the plaques of composite junctions (CJs) in myocardiac intercalated disks (IDs).
24770932	0	71	dep	disks	141:145	arg1	finding					159:165	a unifying finding	148:165	myocardial intercalated disks: a unifying finding for cell biology and cardiology	117:197	Protein LUMA is a cytoplasmic plaque constituent of various epithelial adherens junctions and composite junctions of myocardial intercalated disks: a unifying finding for cell biology and cardiology.
24770932	6	72	theme	pathogenic	1349:1358	arg1	states					1360:1365	the pathogenic states	1345:1365	the pathogenic states of CJs and IDs	1345:1380	The identification of LUMA as a plaque component of myocardiac CJs leads to reconsiderations of the molecular composition and architecture, the development, the functions, and the pathogenic states of CJs and IDs.
27842842	2	0	theme	Fe3+/Fe2+	517:525	arg1	concentration					527:539	the Fe3+/Fe2+ concentration	513:539	the Fe3+/Fe2+ concentration	513:539	When evaluated as electromagnetic interference (EMI) shielding materials, the α-FeOOH/cellulose-derived carbon aerogels composite displays the highest SEtotal value of 34.0dB at the Fe3+/Fe2+ concentration of 0.01M, which is about 4.8 times higher than that of the individual α-FeOOH (5.9dB).
27842842	2	0	theme	Fe3+/Fe2+	517:525	arg1	times					570:574	times	570:574	times	570:574	When evaluated as electromagnetic interference (EMI) shielding materials, the α-FeOOH/cellulose-derived carbon aerogels composite displays the highest SEtotal value of 34.0dB at the Fe3+/Fe2+ concentration of 0.01M, which is about 4.8 times higher than that of the individual α-FeOOH (5.9dB).
27842842	3	1	theme	performance	707:717	arg1	deterioration					719:731	the EMI shielding performance deterioration	689:731	the EMI shielding performance deterioration	689:731	When the higher or lower Fe3+/Fe2+ concentrations were used, the EMI shielding performance deterioration occurred.
27842842	1	2	theme	chemical	166:173	arg1	method					189:194	a rapid and facile chemical precipitation method	147:194	a rapid and facile chemical precipitation method to grow goethite (α-FeOOH) nanoneedles and nanoflowers on the carbon aerogels which was obtained from the pyrolysis of cellulose aerogels	147:332	We describe a rapid and facile chemical precipitation method to grow goethite (α-FeOOH) nanoneedles and nanoflowers on the carbon aerogels which was obtained from the pyrolysis of cellulose aerogels.
27842842	1	3	theme	carbon	258:263	arg1	aerogels					265:272	the carbon aerogels	254:272	the carbon aerogels which was obtained from the pyrolysis of cellulose aerogels	254:332	We describe a rapid and facile chemical precipitation method to grow goethite (α-FeOOH) nanoneedles and nanoflowers on the carbon aerogels which was obtained from the pyrolysis of cellulose aerogels.
27842842	2	4	theme	interference	369:380	arg1	materials					398:406	electromagnetic interference (EMI) shielding materials	353:406	electromagnetic interference (EMI) shielding materials	353:406	When evaluated as electromagnetic interference (EMI) shielding materials, the α-FeOOH/cellulose-derived carbon aerogels composite displays the highest SEtotal value of 34.0dB at the Fe3+/Fe2+ concentration of 0.01M, which is about 4.8 times higher than that of the individual α-FeOOH (5.9dB).
27842842	0	5	theme	interference	111:122	arg1	shielding					124:132	electromagnetic interference shielding	95:132	electromagnetic interference shielding	95:132	Cellulose-derived carbon aerogels supported goethite (α-FeOOH) nanoneedles and nanoflowers for electromagnetic interference shielding.
27842842	3	6	theme	EMI	693:695	arg1	deterioration					719:731	the EMI shielding performance deterioration	689:731	the EMI shielding performance deterioration	689:731	When the higher or lower Fe3+/Fe2+ concentrations were used, the EMI shielding performance deterioration occurred.
27842842	4	7	theme	carbon	779:784	arg1	aerogels					786:793	the carbon aerogels	775:793	the carbon aerogels	775:793	The integration of α-FeOOH with the carbon aerogels transforms the reflection-dominant mechanism for α-FeOOH into the adsorption-dominant mechanism for the composite.
27842842	4	8	theme	α-FeOOH	762:768	arg1	integration					747:757	The integration	743:757	The integration of α-FeOOH with the carbon aerogels	743:793	The integration of α-FeOOH with the carbon aerogels transforms the reflection-dominant mechanism for α-FeOOH into the adsorption-dominant mechanism for the composite.
27842842	0	9	theme	electromagnetic	95:109	arg1	shielding					124:132	electromagnetic interference shielding	95:132	electromagnetic interference shielding	95:132	Cellulose-derived carbon aerogels supported goethite (α-FeOOH) nanoneedles and nanoflowers for electromagnetic interference shielding.
27842842	2	10	theme	individual	600:609	arg1	5.9dB					620:624	5.9dB	620:624	5.9dB	620:624	When evaluated as electromagnetic interference (EMI) shielding materials, the α-FeOOH/cellulose-derived carbon aerogels composite displays the highest SEtotal value of 34.0dB at the Fe3+/Fe2+ concentration of 0.01M, which is about 4.8 times higher than that of the individual α-FeOOH (5.9dB).
27842842	2	10	theme	individual	600:609	arg1	α-FeOOH					611:617	the individual α-FeOOH	596:617	the individual α-FeOOH (5.9dB)	596:625	When evaluated as electromagnetic interference (EMI) shielding materials, the α-FeOOH/cellulose-derived carbon aerogels composite displays the highest SEtotal value of 34.0dB at the Fe3+/Fe2+ concentration of 0.01M, which is about 4.8 times higher than that of the individual α-FeOOH (5.9dB).
27842842	5	11	theme	attractive	1037:1046	arg1	contribution					962:973	contribution	962:973	contribution	962:973	The adsorption-dominant mechanism undoubtedly makes contribution to alleviating secondary radiation, which is regarded as more attractive alternative for developing electromagnetic radiation protection products.
27842842	5	11	theme	attractive	1037:1046	arg1	alternative					1048:1058	more attractive alternative	1032:1058	more attractive alternative for developing electromagnetic radiation protection products	1032:1119	The adsorption-dominant mechanism undoubtedly makes contribution to alleviating secondary radiation, which is regarded as more attractive alternative for developing electromagnetic radiation protection products.
27842842	5	12	theme	alleviating	978:988	arg1	radiation					1000:1008	alleviating secondary radiation	978:1008	alleviating secondary radiation	978:1008	The adsorption-dominant mechanism undoubtedly makes contribution to alleviating secondary radiation, which is regarded as more attractive alternative for developing electromagnetic radiation protection products.
27842842	2	13	theme	aerogels	446:453	arg1	composite					455:463	the α-FeOOH/cellulose-derived carbon aerogels composite	409:463	the α-FeOOH/cellulose-derived carbon aerogels composite	409:463	When evaluated as electromagnetic interference (EMI) shielding materials, the α-FeOOH/cellulose-derived carbon aerogels composite displays the highest SEtotal value of 34.0dB at the Fe3+/Fe2+ concentration of 0.01M, which is about 4.8 times higher than that of the individual α-FeOOH (5.9dB).
27842842	2	14	theme	0.01M	544:548	arg1	concentration					527:539	the Fe3+/Fe2+ concentration	513:539	the Fe3+/Fe2+ concentration	513:539	When evaluated as electromagnetic interference (EMI) shielding materials, the α-FeOOH/cellulose-derived carbon aerogels composite displays the highest SEtotal value of 34.0dB at the Fe3+/Fe2+ concentration of 0.01M, which is about 4.8 times higher than that of the individual α-FeOOH (5.9dB).
27842842	2	14	theme	0.01M	544:548	arg1	times					570:574	times	570:574	times	570:574	When evaluated as electromagnetic interference (EMI) shielding materials, the α-FeOOH/cellulose-derived carbon aerogels composite displays the highest SEtotal value of 34.0dB at the Fe3+/Fe2+ concentration of 0.01M, which is about 4.8 times higher than that of the individual α-FeOOH (5.9dB).
27842842	2	15	theme	highest	478:484	arg1	value					494:498	the highest SEtotal value	474:498	the highest SEtotal value of 34.0dB at the Fe3+/Fe2+ concentration of 0.01M, which is about 4.8 times higher than that of the individual α-FeOOH (5.9dB)	474:625	When evaluated as electromagnetic interference (EMI) shielding materials, the α-FeOOH/cellulose-derived carbon aerogels composite displays the highest SEtotal value of 34.0dB at the Fe3+/Fe2+ concentration of 0.01M, which is about 4.8 times higher than that of the individual α-FeOOH (5.9dB).
27842842	2	16	dep	times	570:574	arg1	higher					576:581	higher	576:581	higher	576:581	When evaluated as electromagnetic interference (EMI) shielding materials, the α-FeOOH/cellulose-derived carbon aerogels composite displays the highest SEtotal value of 34.0dB at the Fe3+/Fe2+ concentration of 0.01M, which is about 4.8 times higher than that of the individual α-FeOOH (5.9dB).
27842842	0	17	theme	carbon	18:23	arg1	aerogels					25:32	Cellulose-derived carbon aerogels	0:32	Cellulose-derived carbon aerogels	0:32	Cellulose-derived carbon aerogels supported goethite (α-FeOOH) nanoneedles and nanoflowers for electromagnetic interference shielding.
27842842	5	18	theme	adsorption-dominant	914:932	arg1	mechanism					934:942	The adsorption-dominant mechanism	910:942	The adsorption-dominant mechanism	910:942	The adsorption-dominant mechanism undoubtedly makes contribution to alleviating secondary radiation, which is regarded as more attractive alternative for developing electromagnetic radiation protection products.
27842842	0	19	theme	Cellulose-derived	0:16	arg1	aerogels					25:32	Cellulose-derived carbon aerogels	0:32	Cellulose-derived carbon aerogels	0:32	Cellulose-derived carbon aerogels supported goethite (α-FeOOH) nanoneedles and nanoflowers for electromagnetic interference shielding.
27842842	1	20	theme	goethite	204:211	arg1	nanoneedles					223:233	goethite (α-FeOOH) nanoneedles	204:233	goethite (α-FeOOH) nanoneedles	204:233	We describe a rapid and facile chemical precipitation method to grow goethite (α-FeOOH) nanoneedles and nanoflowers on the carbon aerogels which was obtained from the pyrolysis of cellulose aerogels.
27842842	0	21	link	Cellulose-derived	0:16	arg1	aerogels					25:32	Cellulose-derived carbon aerogels	0:32	Cellulose-derived carbon aerogels	0:32	Cellulose-derived carbon aerogels supported goethite (α-FeOOH) nanoneedles and nanoflowers for electromagnetic interference shielding.
27842842	3	22	theme	lower	647:651	arg1	concentrations					663:676	the higher or lower Fe3+/Fe2+ concentrations	633:676	the higher or lower Fe3+/Fe2+ concentrations	633:676	When the higher or lower Fe3+/Fe2+ concentrations were used, the EMI shielding performance deterioration occurred.
27842842	1	23	theme	precipitation	175:187	arg1	method					189:194	a rapid and facile chemical precipitation method	147:194	a rapid and facile chemical precipitation method to grow goethite (α-FeOOH) nanoneedles and nanoflowers on the carbon aerogels which was obtained from the pyrolysis of cellulose aerogels	147:332	We describe a rapid and facile chemical precipitation method to grow goethite (α-FeOOH) nanoneedles and nanoflowers on the carbon aerogels which was obtained from the pyrolysis of cellulose aerogels.
27842842	1	24	theme	α-FeOOH	214:220	arg1	nanoneedles					223:233	goethite (α-FeOOH) nanoneedles	204:233	goethite (α-FeOOH) nanoneedles	204:233	We describe a rapid and facile chemical precipitation method to grow goethite (α-FeOOH) nanoneedles and nanoflowers on the carbon aerogels which was obtained from the pyrolysis of cellulose aerogels.
27842842	5	25	theme	protection	1101:1110	arg1	products					1112:1119	electromagnetic radiation protection products	1075:1119	electromagnetic radiation protection products	1075:1119	The adsorption-dominant mechanism undoubtedly makes contribution to alleviating secondary radiation, which is regarded as more attractive alternative for developing electromagnetic radiation protection products.
27842842	2	26	theme	4.8	566:568	arg1	times					570:574	times	570:574	times	570:574	When evaluated as electromagnetic interference (EMI) shielding materials, the α-FeOOH/cellulose-derived carbon aerogels composite displays the highest SEtotal value of 34.0dB at the Fe3+/Fe2+ concentration of 0.01M, which is about 4.8 times higher than that of the individual α-FeOOH (5.9dB).
27842842	2	26	theme	4.8	566:568	arg1	concentration					527:539	the Fe3+/Fe2+ concentration	513:539	the Fe3+/Fe2+ concentration	513:539	When evaluated as electromagnetic interference (EMI) shielding materials, the α-FeOOH/cellulose-derived carbon aerogels composite displays the highest SEtotal value of 34.0dB at the Fe3+/Fe2+ concentration of 0.01M, which is about 4.8 times higher than that of the individual α-FeOOH (5.9dB).
27842842	3	27	theme	Fe3+/Fe2+	653:661	arg1	concentrations					663:676	the higher or lower Fe3+/Fe2+ concentrations	633:676	the higher or lower Fe3+/Fe2+ concentrations	633:676	When the higher or lower Fe3+/Fe2+ concentrations were used, the EMI shielding performance deterioration occurred.
27842842	2	28	theme	shielding	388:396	arg1	materials					398:406	electromagnetic interference (EMI) shielding materials	353:406	electromagnetic interference (EMI) shielding materials	353:406	When evaluated as electromagnetic interference (EMI) shielding materials, the α-FeOOH/cellulose-derived carbon aerogels composite displays the highest SEtotal value of 34.0dB at the Fe3+/Fe2+ concentration of 0.01M, which is about 4.8 times higher than that of the individual α-FeOOH (5.9dB).
27842842	2	29	theme	carbon	439:444	arg1	composite					455:463	the α-FeOOH/cellulose-derived carbon aerogels composite	409:463	the α-FeOOH/cellulose-derived carbon aerogels composite	409:463	When evaluated as electromagnetic interference (EMI) shielding materials, the α-FeOOH/cellulose-derived carbon aerogels composite displays the highest SEtotal value of 34.0dB at the Fe3+/Fe2+ concentration of 0.01M, which is about 4.8 times higher than that of the individual α-FeOOH (5.9dB).
27842842	0	30	theme	goethite	44:51	arg1	nanoneedles					63:73	goethite (α-FeOOH) nanoneedles	44:73	goethite (α-FeOOH) nanoneedles	44:73	Cellulose-derived carbon aerogels supported goethite (α-FeOOH) nanoneedles and nanoflowers for electromagnetic interference shielding.
27842842	2	31	theme	34.0dB	503:508	arg1	value					494:498	the highest SEtotal value	474:498	the highest SEtotal value of 34.0dB at the Fe3+/Fe2+ concentration of 0.01M, which is about 4.8 times higher than that of the individual α-FeOOH (5.9dB)	474:625	When evaluated as electromagnetic interference (EMI) shielding materials, the α-FeOOH/cellulose-derived carbon aerogels composite displays the highest SEtotal value of 34.0dB at the Fe3+/Fe2+ concentration of 0.01M, which is about 4.8 times higher than that of the individual α-FeOOH (5.9dB).
27842842	3	32	theme	higher	637:642	arg1	concentrations					663:676	the higher or lower Fe3+/Fe2+ concentrations	633:676	the higher or lower Fe3+/Fe2+ concentrations	633:676	When the higher or lower Fe3+/Fe2+ concentrations were used, the EMI shielding performance deterioration occurred.
27842842	2	33	theme	α-FeOOH/cellulose-derived	413:437	arg1	composite					455:463	the α-FeOOH/cellulose-derived carbon aerogels composite	409:463	the α-FeOOH/cellulose-derived carbon aerogels composite	409:463	When evaluated as electromagnetic interference (EMI) shielding materials, the α-FeOOH/cellulose-derived carbon aerogels composite displays the highest SEtotal value of 34.0dB at the Fe3+/Fe2+ concentration of 0.01M, which is about 4.8 times higher than that of the individual α-FeOOH (5.9dB).
27842842	1	34	theme	cellulose	315:323	arg1	aerogels					325:332	cellulose aerogels	315:332	cellulose aerogels	315:332	We describe a rapid and facile chemical precipitation method to grow goethite (α-FeOOH) nanoneedles and nanoflowers on the carbon aerogels which was obtained from the pyrolysis of cellulose aerogels.
27842842	2	35	from	concentration	527:539	arg1	value					494:498	the highest SEtotal value	474:498	the highest SEtotal value of 34.0dB at the Fe3+/Fe2+ concentration of 0.01M, which is about 4.8 times higher than that of the individual α-FeOOH (5.9dB)	474:625	When evaluated as electromagnetic interference (EMI) shielding materials, the α-FeOOH/cellulose-derived carbon aerogels composite displays the highest SEtotal value of 34.0dB at the Fe3+/Fe2+ concentration of 0.01M, which is about 4.8 times higher than that of the individual α-FeOOH (5.9dB).
27842842	4	36	theme	adsorption-dominant	861:879	arg1	mechanism					881:889	the adsorption-dominant mechanism	857:889	the adsorption-dominant mechanism for the composite	857:907	The integration of α-FeOOH with the carbon aerogels transforms the reflection-dominant mechanism for α-FeOOH into the adsorption-dominant mechanism for the composite.
27842842	2	37	theme	electromagnetic	353:367	arg1	EMI					383:385	EMI	383:385	EMI	383:385	When evaluated as electromagnetic interference (EMI) shielding materials, the α-FeOOH/cellulose-derived carbon aerogels composite displays the highest SEtotal value of 34.0dB at the Fe3+/Fe2+ concentration of 0.01M, which is about 4.8 times higher than that of the individual α-FeOOH (5.9dB).
27842842	2	37	theme	electromagnetic	353:367	arg1	interference					369:380	electromagnetic interference	353:380	electromagnetic interference (EMI) shielding materials	353:406	When evaluated as electromagnetic interference (EMI) shielding materials, the α-FeOOH/cellulose-derived carbon aerogels composite displays the highest SEtotal value of 34.0dB at the Fe3+/Fe2+ concentration of 0.01M, which is about 4.8 times higher than that of the individual α-FeOOH (5.9dB).
27842842	0	38	theme	α-FeOOH	54:60	arg1	nanoneedles					63:73	goethite (α-FeOOH) nanoneedles	44:73	goethite (α-FeOOH) nanoneedles	44:73	Cellulose-derived carbon aerogels supported goethite (α-FeOOH) nanoneedles and nanoflowers for electromagnetic interference shielding.
27842842	1	39	theme	aerogels	325:332	arg1	pyrolysis					302:310	the pyrolysis	298:310	the pyrolysis of cellulose aerogels	298:332	We describe a rapid and facile chemical precipitation method to grow goethite (α-FeOOH) nanoneedles and nanoflowers on the carbon aerogels which was obtained from the pyrolysis of cellulose aerogels.
27842842	5	40	theme	secondary	990:998	arg1	radiation					1000:1008	alleviating secondary radiation	978:1008	alleviating secondary radiation	978:1008	The adsorption-dominant mechanism undoubtedly makes contribution to alleviating secondary radiation, which is regarded as more attractive alternative for developing electromagnetic radiation protection products.
27842842	3	41	used	used	683:686	arg2	concentrations					663:676	the higher or lower Fe3+/Fe2+ concentrations	633:676	the higher or lower Fe3+/Fe2+ concentrations	633:676	When the higher or lower Fe3+/Fe2+ concentrations were used, the EMI shielding performance deterioration occurred.
27842842	2	42	link	α-FeOOH/cellulose-derived	413:437	arg1	composite					455:463	the α-FeOOH/cellulose-derived carbon aerogels composite	409:463	the α-FeOOH/cellulose-derived carbon aerogels composite	409:463	When evaluated as electromagnetic interference (EMI) shielding materials, the α-FeOOH/cellulose-derived carbon aerogels composite displays the highest SEtotal value of 34.0dB at the Fe3+/Fe2+ concentration of 0.01M, which is about 4.8 times higher than that of the individual α-FeOOH (5.9dB).
27842842	4	43	theme	reflection-dominant	810:828	arg1	mechanism					830:838	the reflection-dominant mechanism	806:838	the reflection-dominant mechanism for α-FeOOH	806:850	The integration of α-FeOOH with the carbon aerogels transforms the reflection-dominant mechanism for α-FeOOH into the adsorption-dominant mechanism for the composite.
27842842	5	44	theme	electromagnetic	1075:1089	arg1	products					1112:1119	electromagnetic radiation protection products	1075:1119	electromagnetic radiation protection products	1075:1119	The adsorption-dominant mechanism undoubtedly makes contribution to alleviating secondary radiation, which is regarded as more attractive alternative for developing electromagnetic radiation protection products.
27842842	1	45	theme	rapid	149:153	arg1	method					189:194	a rapid and facile chemical precipitation method	147:194	a rapid and facile chemical precipitation method to grow goethite (α-FeOOH) nanoneedles and nanoflowers on the carbon aerogels which was obtained from the pyrolysis of cellulose aerogels	147:332	We describe a rapid and facile chemical precipitation method to grow goethite (α-FeOOH) nanoneedles and nanoflowers on the carbon aerogels which was obtained from the pyrolysis of cellulose aerogels.
27842842	5	46	theme	radiation	1091:1099	arg1	products					1112:1119	electromagnetic radiation protection products	1075:1119	electromagnetic radiation protection products	1075:1119	The adsorption-dominant mechanism undoubtedly makes contribution to alleviating secondary radiation, which is regarded as more attractive alternative for developing electromagnetic radiation protection products.
27842842	2	47	theme	SEtotal	486:492	arg1	value					494:498	the highest SEtotal value	474:498	the highest SEtotal value of 34.0dB at the Fe3+/Fe2+ concentration of 0.01M, which is about 4.8 times higher than that of the individual α-FeOOH (5.9dB)	474:625	When evaluated as electromagnetic interference (EMI) shielding materials, the α-FeOOH/cellulose-derived carbon aerogels composite displays the highest SEtotal value of 34.0dB at the Fe3+/Fe2+ concentration of 0.01M, which is about 4.8 times higher than that of the individual α-FeOOH (5.9dB).
27842842	3	48	theme	shielding	697:705	arg1	deterioration					719:731	the EMI shielding performance deterioration	689:731	the EMI shielding performance deterioration	689:731	When the higher or lower Fe3+/Fe2+ concentrations were used, the EMI shielding performance deterioration occurred.
27842842	4	49	with	integration	747:757	arg1	aerogels					786:793	the carbon aerogels	775:793	the carbon aerogels	775:793	The integration of α-FeOOH with the carbon aerogels transforms the reflection-dominant mechanism for α-FeOOH into the adsorption-dominant mechanism for the composite.
27842842	1	50	theme	facile	159:164	arg1	method					189:194	a rapid and facile chemical precipitation method	147:194	a rapid and facile chemical precipitation method to grow goethite (α-FeOOH) nanoneedles and nanoflowers on the carbon aerogels which was obtained from the pyrolysis of cellulose aerogels	147:332	We describe a rapid and facile chemical precipitation method to grow goethite (α-FeOOH) nanoneedles and nanoflowers on the carbon aerogels which was obtained from the pyrolysis of cellulose aerogels.
26249574	1	0	theme	important	170:178	arg1	procedure					180:188	an important procedure	167:188	an important procedure	167:188	This study investigates effective immobilization of proteins, an important procedure in many fields of bioengineering and medicine, using various biomaterials.
26249574	4	1	from	composites	717:726	arg1	general					731:737	general	731:737	general	731:737	Electrochemical impedance spectroscopy analysis revealed gelatin composites in general to exhibit better immobilization performance relative to the native gelatin which can be attributed to enhanced film morphologies of the composite structures.
26249574	0	2	theme	hydrogel	82:89	arg1	biomaterials					91:102	various carbon nanotube embedded hydrogel biomaterials	49:102	various carbon nanotube embedded hydrogel biomaterials	49:102	Evaluation of protein immobilization capacity on various carbon nanotube embedded hydrogel biomaterials.
26249574	5	3	theme	groups	1143:1148	arg1	presence					1113:1120	the presence	1109:1120	the presence of structural active groups	1109:1148	Moreover, superior immobilization efficiencies were obtained with the addition of carbon nanotubes, due to their conducting and surface enhancement features, especially in the gelatin-chitosan structures due to the presence of structural active groups.
26249574	0	4	from	Evaluation	0:9	arg1	biomaterials					91:102	various carbon nanotube embedded hydrogel biomaterials	49:102	various carbon nanotube embedded hydrogel biomaterials	49:102	Evaluation of protein immobilization capacity on various carbon nanotube embedded hydrogel biomaterials.
26249574	1	5	from	procedure	180:188	arg1	fields					198:203	many fields	193:203	many fields of bioengineering and medicine	193:234	This study investigates effective immobilization of proteins, an important procedure in many fields of bioengineering and medicine, using various biomaterials.
26249574	4	6	theme	native	800:805	arg1	gelatin					807:813	the native gelatin	796:813	the native gelatin which can be attributed to enhanced film morphologies of the composite structures	796:895	Electrochemical impedance spectroscopy analysis revealed gelatin composites in general to exhibit better immobilization performance relative to the native gelatin which can be attributed to enhanced film morphologies of the composite structures.
26249574	4	7	theme	impedance	668:676	arg1	analysis					691:698	Electrochemical impedance spectroscopy analysis	652:698	Electrochemical impedance spectroscopy analysis	652:698	Electrochemical impedance spectroscopy analysis revealed gelatin composites in general to exhibit better immobilization performance relative to the native gelatin which can be attributed to enhanced film morphologies of the composite structures.
26249574	5	8	theme	nanotubes	987:995	arg1	addition					968:975	the addition	964:975	the addition of carbon nanotubes	964:995	Moreover, superior immobilization efficiencies were obtained with the addition of carbon nanotubes, due to their conducting and surface enhancement features, especially in the gelatin-chitosan structures due to the presence of structural active groups.
26249574	1	9	dep	proteins	157:164	arg1	using					237:241	using	237:241	using various biomaterials	237:262	This study investigates effective immobilization of proteins, an important procedure in many fields of bioengineering and medicine, using various biomaterials.
26249574	3	10	theme	electron	510:517	arg1	microscopy					519:528	scanning electron microscopy	501:528	scanning electron microscopy (SEM)	501:534	The prepared nano/composite structures were characterized using scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TG) and contact angle analysis (CA).
26249574	3	10	theme	electron	510:517	arg1	SEM					531:533	SEM	531:533	SEM	531:533	The prepared nano/composite structures were characterized using scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TG) and contact angle analysis (CA).
26249574	4	11	theme	composite	876:884	arg1	structures					886:895	the composite structures	872:895	the composite structures	872:895	Electrochemical impedance spectroscopy analysis revealed gelatin composites in general to exhibit better immobilization performance relative to the native gelatin which can be attributed to enhanced film morphologies of the composite structures.
26249574	3	12	theme	infrared	555:562	arg1	FTIR					578:581	FTIR	578:581	FTIR	578:581	The prepared nano/composite structures were characterized using scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TG) and contact angle analysis (CA).
26249574	3	12	theme	infrared	555:562	arg1	spectroscopy					564:575	Fourier-transform infrared spectroscopy	537:575	Fourier-transform infrared spectroscopy (FTIR)	537:582	The prepared nano/composite structures were characterized using scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TG) and contact angle analysis (CA).
26249574	2	13	theme	carbon	412:417	arg1	CNTs					430:433	CNTs	430:433	CNTs	430:433	Gelatin, alginate and chitosan were chosen as polymeric carriers, and applied in both their composites and nanocomposite forms in combination with carbon nanotubes (CNTs).
26249574	2	13	theme	carbon	412:417	arg1	nanotubes					419:427	carbon nanotubes	412:427	carbon nanotubes (CNTs)	412:434	Gelatin, alginate and chitosan were chosen as polymeric carriers, and applied in both their composites and nanocomposite forms in combination with carbon nanotubes (CNTs).
26249574	5	14	theme	surface	1026:1032	arg1	features					1046:1053	their conducting and surface enhancement features	1005:1053	features	1046:1053	Moreover, superior immobilization efficiencies were obtained with the addition of carbon nanotubes, due to their conducting and surface enhancement features, especially in the gelatin-chitosan structures due to the presence of structural active groups.
26249574	1	15	theme	many	193:196	arg1	fields					198:203	many fields	193:203	many fields of bioengineering and medicine	193:234	This study investigates effective immobilization of proteins, an important procedure in many fields of bioengineering and medicine, using various biomaterials.
26249574	3	16	theme	Fourier-transform	537:553	arg1	FTIR					578:581	FTIR	578:581	FTIR	578:581	The prepared nano/composite structures were characterized using scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TG) and contact angle analysis (CA).
26249574	3	16	theme	Fourier-transform	537:553	arg1	spectroscopy					564:575	Fourier-transform infrared spectroscopy	537:575	Fourier-transform infrared spectroscopy (FTIR)	537:582	The prepared nano/composite structures were characterized using scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TG) and contact angle analysis (CA).
26249574	2	17	from	composites	357:366	arg1	combination					395:405	combination	395:405	combination with carbon nanotubes (CNTs)	395:434	Gelatin, alginate and chitosan were chosen as polymeric carriers, and applied in both their composites and nanocomposite forms in combination with carbon nanotubes (CNTs).
26249574	3	18	theme	thermal	585:591	arg1	TG					615:616	TG	615:616	TG	615:616	The prepared nano/composite structures were characterized using scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TG) and contact angle analysis (CA).
26249574	3	18	theme	thermal	585:591	arg1	analysis					605:612	thermal gravimetric analysis	585:612	thermal gravimetric analysis (TG)	585:617	The prepared nano/composite structures were characterized using scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TG) and contact angle analysis (CA).
26249574	5	19	theme	superior	908:915	arg1	efficiencies					932:943	superior immobilization efficiencies	908:943	superior immobilization efficiencies	908:943	Moreover, superior immobilization efficiencies were obtained with the addition of carbon nanotubes, due to their conducting and surface enhancement features, especially in the gelatin-chitosan structures due to the presence of structural active groups.
26249574	4	20	theme	structures	886:895	arg1	morphologies					856:867	enhanced film morphologies	842:867	enhanced film morphologies of the composite structures	842:895	Electrochemical impedance spectroscopy analysis revealed gelatin composites in general to exhibit better immobilization performance relative to the native gelatin which can be attributed to enhanced film morphologies of the composite structures.
26249574	2	21	from	forms	386:390	arg1	combination					395:405	combination	395:405	combination with carbon nanotubes (CNTs)	395:434	Gelatin, alginate and chitosan were chosen as polymeric carriers, and applied in both their composites and nanocomposite forms in combination with carbon nanotubes (CNTs).
26249574	3	22	theme	gravimetric	593:603	arg1	TG					615:616	TG	615:616	TG	615:616	The prepared nano/composite structures were characterized using scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TG) and contact angle analysis (CA).
26249574	3	22	theme	gravimetric	593:603	arg1	analysis					605:612	thermal gravimetric analysis	585:612	thermal gravimetric analysis (TG)	585:617	The prepared nano/composite structures were characterized using scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TG) and contact angle analysis (CA).
26249574	4	23	theme	spectroscopy	678:689	arg1	analysis					691:698	Electrochemical impedance spectroscopy analysis	652:698	Electrochemical impedance spectroscopy analysis	652:698	Electrochemical impedance spectroscopy analysis revealed gelatin composites in general to exhibit better immobilization performance relative to the native gelatin which can be attributed to enhanced film morphologies of the composite structures.
26249574	0	24	theme	immobilization	22:35	arg1	capacity					37:44	protein immobilization capacity	14:44	protein immobilization capacity	14:44	Evaluation of protein immobilization capacity on various carbon nanotube embedded hydrogel biomaterials.
26249574	4	25	theme	immobilization	757:770	arg1	performance					772:782	better immobilization performance	750:782	better immobilization performance relative to the native gelatin which can be attributed to enhanced film morphologies of the composite structures	750:895	Electrochemical impedance spectroscopy analysis revealed gelatin composites in general to exhibit better immobilization performance relative to the native gelatin which can be attributed to enhanced film morphologies of the composite structures.
26249574	5	26	theme	carbon	980:985	arg1	nanotubes					987:995	carbon nanotubes	980:995	carbon nanotubes	980:995	Moreover, superior immobilization efficiencies were obtained with the addition of carbon nanotubes, due to their conducting and surface enhancement features, especially in the gelatin-chitosan structures due to the presence of structural active groups.
26249574	1	27	theme	bioengineering	208:221	arg1	fields					198:203	many fields	193:203	many fields of bioengineering and medicine	193:234	This study investigates effective immobilization of proteins, an important procedure in many fields of bioengineering and medicine, using various biomaterials.
26249574	0	28	theme	protein	14:20	arg1	capacity					37:44	protein immobilization capacity	14:44	protein immobilization capacity	14:44	Evaluation of protein immobilization capacity on various carbon nanotube embedded hydrogel biomaterials.
26249574	2	29	theme	polymeric	311:319	arg1	carriers					321:328	polymeric carriers	311:328	polymeric carriers	311:328	Gelatin, alginate and chitosan were chosen as polymeric carriers, and applied in both their composites and nanocomposite forms in combination with carbon nanotubes (CNTs).
26249574	2	29	theme	polymeric	311:319	arg1	chitosan					287:294	chitosan	287:294	chitosan	287:294	Gelatin, alginate and chitosan were chosen as polymeric carriers, and applied in both their composites and nanocomposite forms in combination with carbon nanotubes (CNTs).
26249574	2	29	theme	polymeric	311:319	arg1	alginate					274:281	alginate	274:281	alginate	274:281	Gelatin, alginate and chitosan were chosen as polymeric carriers, and applied in both their composites and nanocomposite forms in combination with carbon nanotubes (CNTs).
26249574	2	29	theme	polymeric	311:319	arg1	Gelatin					265:271	Gelatin	265:271	Gelatin	265:271	Gelatin, alginate and chitosan were chosen as polymeric carriers, and applied in both their composites and nanocomposite forms in combination with carbon nanotubes (CNTs).
26249574	4	30	theme	Electrochemical	652:666	arg1	analysis					691:698	Electrochemical impedance spectroscopy analysis	652:698	Electrochemical impedance spectroscopy analysis	652:698	Electrochemical impedance spectroscopy analysis revealed gelatin composites in general to exhibit better immobilization performance relative to the native gelatin which can be attributed to enhanced film morphologies of the composite structures.
26249574	5	31	theme	structural	1125:1134	arg1	groups					1143:1148	structural active groups	1125:1148	structural active groups	1125:1148	Moreover, superior immobilization efficiencies were obtained with the addition of carbon nanotubes, due to their conducting and surface enhancement features, especially in the gelatin-chitosan structures due to the presence of structural active groups.
26249574	4	32	theme	film	851:854	arg1	morphologies					856:867	enhanced film morphologies	842:867	enhanced film morphologies of the composite structures	842:895	Electrochemical impedance spectroscopy analysis revealed gelatin composites in general to exhibit better immobilization performance relative to the native gelatin which can be attributed to enhanced film morphologies of the composite structures.
26249574	5	33	theme	immobilization	917:930	arg1	efficiencies					932:943	superior immobilization efficiencies	908:943	superior immobilization efficiencies	908:943	Moreover, superior immobilization efficiencies were obtained with the addition of carbon nanotubes, due to their conducting and surface enhancement features, especially in the gelatin-chitosan structures due to the presence of structural active groups.
26249574	1	34	theme	medicine	227:234	arg1	fields					198:203	many fields	193:203	many fields of bioengineering and medicine	193:234	This study investigates effective immobilization of proteins, an important procedure in many fields of bioengineering and medicine, using various biomaterials.
26249574	0	35	theme	capacity	37:44	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of protein immobilization capacity on various carbon nanotube embedded hydrogel biomaterials.	0:103	Evaluation of protein immobilization capacity on various carbon nanotube embedded hydrogel biomaterials.
26249574	5	36	theme	active	1136:1141	arg1	groups					1143:1148	structural active groups	1125:1148	structural active groups	1125:1148	Moreover, superior immobilization efficiencies were obtained with the addition of carbon nanotubes, due to their conducting and surface enhancement features, especially in the gelatin-chitosan structures due to the presence of structural active groups.
26249574	4	37	theme	enhanced	842:849	arg1	morphologies					856:867	enhanced film morphologies	842:867	enhanced film morphologies of the composite structures	842:895	Electrochemical impedance spectroscopy analysis revealed gelatin composites in general to exhibit better immobilization performance relative to the native gelatin which can be attributed to enhanced film morphologies of the composite structures.
26249574	0	38	theme	carbon	57:62	arg1	biomaterials					91:102	various carbon nanotube embedded hydrogel biomaterials	49:102	various carbon nanotube embedded hydrogel biomaterials	49:102	Evaluation of protein immobilization capacity on various carbon nanotube embedded hydrogel biomaterials.
26249574	3	39	theme	prepared	441:448	arg1	structures					465:474	The prepared nano/composite structures	437:474	The prepared nano/composite structures	437:474	The prepared nano/composite structures were characterized using scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TG) and contact angle analysis (CA).
26249574	5	40	theme	gelatin-chitosan	1074:1089	arg1	structures					1091:1100	the gelatin-chitosan structures	1070:1100	the gelatin-chitosan structures due to the presence of structural active groups	1070:1148	Moreover, superior immobilization efficiencies were obtained with the addition of carbon nanotubes, due to their conducting and surface enhancement features, especially in the gelatin-chitosan structures due to the presence of structural active groups.
26249574	1	41	theme	effective	129:137	arg1	immobilization					139:152	effective immobilization	129:152	effective immobilization of proteins, an important procedure in many fields of bioengineering and medicine, using various biomaterials	129:262	This study investigates effective immobilization of proteins, an important procedure in many fields of bioengineering and medicine, using various biomaterials.
26249574	0	42	theme	various	49:55	arg1	biomaterials					91:102	various carbon nanotube embedded hydrogel biomaterials	49:102	various carbon nanotube embedded hydrogel biomaterials	49:102	Evaluation of protein immobilization capacity on various carbon nanotube embedded hydrogel biomaterials.
26249574	5	43	theme	due	1102:1104	arg1	structures					1091:1100	the gelatin-chitosan structures	1070:1100	the gelatin-chitosan structures due to the presence of structural active groups	1070:1148	Moreover, superior immobilization efficiencies were obtained with the addition of carbon nanotubes, due to their conducting and surface enhancement features, especially in the gelatin-chitosan structures due to the presence of structural active groups.
26249574	4	44	theme	better	750:755	arg1	performance					772:782	better immobilization performance	750:782	better immobilization performance relative to the native gelatin which can be attributed to enhanced film morphologies of the composite structures	750:895	Electrochemical impedance spectroscopy analysis revealed gelatin composites in general to exhibit better immobilization performance relative to the native gelatin which can be attributed to enhanced film morphologies of the composite structures.
26249574	5	45	theme	enhancement	1034:1044	arg1	features					1046:1053	their conducting and surface enhancement features	1005:1053	features	1046:1053	Moreover, superior immobilization efficiencies were obtained with the addition of carbon nanotubes, due to their conducting and surface enhancement features, especially in the gelatin-chitosan structures due to the presence of structural active groups.
26249574	3	46	theme	angle	631:635	arg1	CA					647:648	CA	647:648	CA	647:648	The prepared nano/composite structures were characterized using scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TG) and contact angle analysis (CA).
26249574	3	46	theme	angle	631:635	arg1	analysis					637:644	contact angle analysis	623:644	contact angle analysis (CA)	623:649	The prepared nano/composite structures were characterized using scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TG) and contact angle analysis (CA).
26249574	3	47	theme	scanning	501:508	arg1	microscopy					519:528	scanning electron microscopy	501:528	scanning electron microscopy (SEM)	501:534	The prepared nano/composite structures were characterized using scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TG) and contact angle analysis (CA).
26249574	3	47	theme	scanning	501:508	arg1	SEM					531:533	SEM	531:533	SEM	531:533	The prepared nano/composite structures were characterized using scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TG) and contact angle analysis (CA).
26249574	4	48	theme	relative	784:791	arg1	performance					772:782	better immobilization performance	750:782	better immobilization performance relative to the native gelatin which can be attributed to enhanced film morphologies of the composite structures	750:895	Electrochemical impedance spectroscopy analysis revealed gelatin composites in general to exhibit better immobilization performance relative to the native gelatin which can be attributed to enhanced film morphologies of the composite structures.
26249574	1	49	theme	proteins	157:164	arg1	immobilization					139:152	effective immobilization	129:152	effective immobilization of proteins, an important procedure in many fields of bioengineering and medicine, using various biomaterials	129:262	This study investigates effective immobilization of proteins, an important procedure in many fields of bioengineering and medicine, using various biomaterials.
26249574	1	50	theme	various	243:249	arg1	biomaterials					251:262	various biomaterials	243:262	various biomaterials	243:262	This study investigates effective immobilization of proteins, an important procedure in many fields of bioengineering and medicine, using various biomaterials.
26249574	0	51	theme	embedded	73:80	arg1	biomaterials					91:102	various carbon nanotube embedded hydrogel biomaterials	49:102	various carbon nanotube embedded hydrogel biomaterials	49:102	Evaluation of protein immobilization capacity on various carbon nanotube embedded hydrogel biomaterials.
26249574	4	52	theme	gelatin	709:715	arg1	composites					717:726	gelatin composites	709:726	gelatin composites in general	709:737	Electrochemical impedance spectroscopy analysis revealed gelatin composites in general to exhibit better immobilization performance relative to the native gelatin which can be attributed to enhanced film morphologies of the composite structures.
26249574	3	53	theme	nano/composite	450:463	arg1	structures					465:474	The prepared nano/composite structures	437:474	The prepared nano/composite structures	437:474	The prepared nano/composite structures were characterized using scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TG) and contact angle analysis (CA).
26249574	0	54	theme	nanotube	64:71	arg1	biomaterials					91:102	various carbon nanotube embedded hydrogel biomaterials	49:102	various carbon nanotube embedded hydrogel biomaterials	49:102	Evaluation of protein immobilization capacity on various carbon nanotube embedded hydrogel biomaterials.
26249574	2	55	with	combination	395:405	arg1	CNTs					430:433	CNTs	430:433	CNTs	430:433	Gelatin, alginate and chitosan were chosen as polymeric carriers, and applied in both their composites and nanocomposite forms in combination with carbon nanotubes (CNTs).
26249574	2	55	with	combination	395:405	arg1	nanotubes					419:427	carbon nanotubes	412:427	carbon nanotubes (CNTs)	412:434	Gelatin, alginate and chitosan were chosen as polymeric carriers, and applied in both their composites and nanocomposite forms in combination with carbon nanotubes (CNTs).
26249574	3	56	theme	contact	623:629	arg1	CA					647:648	CA	647:648	CA	647:648	The prepared nano/composite structures were characterized using scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TG) and contact angle analysis (CA).
26249574	3	56	theme	contact	623:629	arg1	analysis					637:644	contact angle analysis	623:644	contact angle analysis (CA)	623:649	The prepared nano/composite structures were characterized using scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TG) and contact angle analysis (CA).
26249574	2	57	theme	nanocomposite	372:384	arg1	forms					386:390	nanocomposite forms	372:390	nanocomposite forms	372:390	Gelatin, alginate and chitosan were chosen as polymeric carriers, and applied in both their composites and nanocomposite forms in combination with carbon nanotubes (CNTs).
26355811	0	0	theme	oxides-based	70:81	arg1	material					100:107	graphite oxides-based solid extraction material	61:107	graphite oxides-based solid extraction material	61:107	Adsorption and desorption of DNA tuned by hydroxyl groups in graphite oxides-based solid extraction material.
26355811	6	1	theme	phenol	855:860	arg1	type					862:865	phenol type	855:865	phenol type	855:865	GO/Cellulose composite provides the major adsorption and desorption of DNA by COH, which belongs to alkyl or phenol type of OH functional group.
26355811	9	2	theme	0-4.15	1229:1234	arg1	concentration					1165:1177	The concentration	1161:1177	The concentration of GO within the composites	1161:1205	The concentration of GO within the composites were controlled to be 0-4.15 wt.
26355811	9	2	theme	0-4.15	1229:1234	arg1	wt					1236:1237	0-4.15 wt	1229:1237	0-4.15 wt	1229:1237	The concentration of GO within the composites were controlled to be 0-4.15 wt.
26355811	0	3	theme	graphite	61:68	arg1	material					100:107	graphite oxides-based solid extraction material	61:107	graphite oxides-based solid extraction material	61:107	Adsorption and desorption of DNA tuned by hydroxyl groups in graphite oxides-based solid extraction material.
26355811	15	4	theme	extraction	1746:1755	arg1	efficiency					1757:1766	high extraction efficiency	1741:1766	high extraction efficiency of pure DNA	1741:1778	The results proved that GO/cellulose composite provides a simple method for selective DNA extraction with high extraction efficiency of pure DNA.
26355811	1	5	theme	crucial	144:150	arg1	method					152:157	the most crucial method	135:157	the most crucial method used in molecular biology	135:183	The extraction of DNA is the most crucial method used in molecular biology.
26355811	1	5	theme	crucial	144:150	arg1	extraction					114:123	The extraction	110:123	The extraction of DNA	110:130	The extraction of DNA is the most crucial method used in molecular biology.
26355811	15	6	theme	high	1741:1744	arg1	efficiency					1757:1766	high extraction efficiency	1741:1766	high extraction efficiency of pure DNA	1741:1778	The results proved that GO/cellulose composite provides a simple method for selective DNA extraction with high extraction efficiency of pure DNA.
26355811	0	7	theme	extraction	89:98	arg1	material					100:107	graphite oxides-based solid extraction material	61:107	graphite oxides-based solid extraction material	61:107	Adsorption and desorption of DNA tuned by hydroxyl groups in graphite oxides-based solid extraction material.
26355811	7	8	theme	elution	1021:1027	arg1	process					1029:1035	the elution process	1017:1035	the elution process	1017:1035	Compared to SiOH, COH is less polarized and reactive, therefore the composite might provide a higher desorption of DNA during the elution process.
26355811	2	9	theme	selective	258:266	arg1	adsorption					272:281	selective DNA adsorption	258:281	selective DNA adsorption	258:281	Up to date silica matrices has been widely applied as solid support for selective DNA adsorption and extraction.
26355811	4	10	theme	functional	541:550	arg1	groups					552:557	different functional groups	531:557	different functional groups which possess of greater desorption property	531:602	In order to increase the DNA extraction yield, a new surface with different functional groups which possess of greater desorption property is required.
26355811	3	11	theme	SiOH	334:337	arg1	groups					350:355	SiOH functional groups	334:355	SiOH functional groups	334:355	However, since adsorption force of SiOH functional groups is much greater than that of desorption force, the DNA extraction efficiency of silica surfaces is limited.
26355811	15	12	theme	DNA	1721:1723	arg1	extraction					1725:1734	selective DNA extraction	1711:1734	selective DNA extraction with high extraction efficiency of pure DNA	1711:1778	The results proved that GO/cellulose composite provides a simple method for selective DNA extraction with high extraction efficiency of pure DNA.
26355811	3	13	theme	surfaces	444:451	arg1	efficiency					423:432	the DNA extraction efficiency	404:432	the DNA extraction efficiency of silica surfaces	404:451	However, since adsorption force of SiOH functional groups is much greater than that of desorption force, the DNA extraction efficiency of silica surfaces is limited.
26355811	3	14	theme	adsorption	314:323	arg1	force					325:329	adsorption force	314:329	adsorption force of SiOH functional groups	314:355	However, since adsorption force of SiOH functional groups is much greater than that of desorption force, the DNA extraction efficiency of silica surfaces is limited.
26355811	6	15	theme	GO/Cellulose	746:757	arg1	composite					759:767	GO/Cellulose composite	746:767	GO/Cellulose composite	746:767	GO/Cellulose composite provides the major adsorption and desorption of DNA by COH, which belongs to alkyl or phenol type of OH functional group.
26355811	15	16	with	extraction	1725:1734	arg1	efficiency					1757:1766	high extraction efficiency	1741:1766	high extraction efficiency of pure DNA	1741:1778	The results proved that GO/cellulose composite provides a simple method for selective DNA extraction with high extraction efficiency of pure DNA.
26355811	15	17	theme	GO/cellulose	1659:1670	arg1	composite					1672:1680	GO/cellulose composite	1659:1680	GO/cellulose composite	1659:1680	The results proved that GO/cellulose composite provides a simple method for selective DNA extraction with high extraction efficiency of pure DNA.
26355811	4	18	theme	different	531:539	arg1	groups					552:557	different functional groups	531:557	different functional groups which possess of greater desorption property	531:602	In order to increase the DNA extraction yield, a new surface with different functional groups which possess of greater desorption property is required.
26355811	9	19	theme	GO	1182:1183	arg1	concentration					1165:1177	The concentration	1161:1177	The concentration of GO within the composites	1161:1205	The concentration of GO within the composites were controlled to be 0-4.15 wt.
26355811	9	19	theme	GO	1182:1183	arg1	wt					1236:1237	0-4.15 wt	1229:1237	0-4.15 wt	1229:1237	The concentration of GO within the composites were controlled to be 0-4.15 wt.
26355811	2	20	theme	solid	240:244	arg1	Up					186:187	Up	186:187	Up to date silica matrices	186:211	Up to date silica matrices has been widely applied as solid support for selective DNA adsorption and extraction.
26355811	2	20	theme	solid	240:244	arg1	support					246:252	solid support	240:252	solid support for selective DNA adsorption and extraction	240:296	Up to date silica matrices has been widely applied as solid support for selective DNA adsorption and extraction.
26355811	6	21	theme	OH	870:871	arg1	group					884:888	OH functional group	870:888	OH functional group	870:888	GO/Cellulose composite provides the major adsorption and desorption of DNA by COH, which belongs to alkyl or phenol type of OH functional group.
26355811	5	22	theme	DNA	715:717	arg1	adsorption					719:728	DNA adsorption	715:728	DNA adsorption	715:728	In this study, we proposed cellulose/graphite oxide (GO) composite as an alternative material for DNA adsorption and extraction.
26355811	15	23	theme	pure	1771:1774	arg1	DNA					1776:1778	pure DNA	1771:1778	pure DNA	1771:1778	The results proved that GO/cellulose composite provides a simple method for selective DNA extraction with high extraction efficiency of pure DNA.
26355811	14	24	theme	absorbance	1479:1488	arg1	1.86					1574:1577	1.86	1574:1577	1.86	1574:1577	The absorbance ratios between 260 nm and 280 nm (A260/A280) of the DNA elution was demonstrated as 1.86, indicating the extracted DNA consisted of high purity.
26355811	14	24	theme	absorbance	1479:1488	arg1	ratios					1490:1495	The absorbance ratios	1475:1495	The absorbance ratios between 260 nm and 280 nm (A260/A280) of the DNA elution	1475:1552	The absorbance ratios between 260 nm and 280 nm (A260/A280) of the DNA elution was demonstrated as 1.86, indicating the extracted DNA consisted of high purity.
26355811	13	25	theme	2M	1443:1444	arg1	buffer					1467:1472	the binding buffer	1455:1472	the binding buffer	1455:1472	% GO, where the extraction efficiency was reported as 660.4 ng/μl when applying 2M GuHCl as the binding buffer.
26355811	13	25	theme	2M	1443:1444	arg1	GuHCl					1446:1450	2M GuHCl	1443:1450	2M GuHCl	1443:1450	% GO, where the extraction efficiency was reported as 660.4 ng/μl when applying 2M GuHCl as the binding buffer.
26355811	3	26	theme	desorption	386:395	arg1	force					397:401	desorption force	386:401	desorption force	386:401	However, since adsorption force of SiOH functional groups is much greater than that of desorption force, the DNA extraction efficiency of silica surfaces is limited.
26355811	8	27	theme	mixing	1105:1110	arg1	urea					1112:1115	mixing urea	1105:1115	mixing urea	1105:1115	The GO/cellulose composite were prepared in spherical structure by mixing urea, cellulose, NaOH, Graphite oxide and water.
26355811	6	28	dep	adsorption	788:797	arg1	the					778:780	the	778:780	the	778:780	GO/Cellulose composite provides the major adsorption and desorption of DNA by COH, which belongs to alkyl or phenol type of OH functional group.
26355811	7	29	theme	DNA	1006:1008	arg1	desorption					992:1001	a higher desorption	983:1001	a higher desorption of DNA	983:1008	Compared to SiOH, COH is less polarized and reactive, therefore the composite might provide a higher desorption of DNA during the elution process.
26355811	5	30	dep	oxide	663:667	arg1	GO					670:671	GO	670:671	GO	670:671	In this study, we proposed cellulose/graphite oxide (GO) composite as an alternative material for DNA adsorption and extraction.
26355811	11	31	theme	weight	1296:1301	arg1	percentage					1303:1312	weight percentage	1296:1312	weight percentage of GO	1296:1318	The extraction yield of DNA increased with increasing weight percentage of GO.
26355811	1	32	theme	molecular	167:175	arg1	biology					177:183	molecular biology	167:183	molecular biology	167:183	The extraction of DNA is the most crucial method used in molecular biology.
26355811	3	33	theme	functional	339:348	arg1	groups					350:355	SiOH functional groups	334:355	SiOH functional groups	334:355	However, since adsorption force of SiOH functional groups is much greater than that of desorption force, the DNA extraction efficiency of silica surfaces is limited.
26355811	5	34	theme	cellulose/graphite	644:661	arg1	oxide					663:667	cellulose/graphite oxide	644:667	cellulose/graphite oxide (GO) composite	644:682	In this study, we proposed cellulose/graphite oxide (GO) composite as an alternative material for DNA adsorption and extraction.
26355811	15	35	theme	selective	1711:1719	arg1	extraction					1725:1734	selective DNA extraction	1711:1734	selective DNA extraction with high extraction efficiency of pure DNA	1711:1778	The results proved that GO/cellulose composite provides a simple method for selective DNA extraction with high extraction efficiency of pure DNA.
26355811	11	36	theme	DNA	1266:1268	arg1	yield					1257:1261	The extraction yield	1242:1261	The extraction yield of DNA	1242:1268	The extraction yield of DNA increased with increasing weight percentage of GO.
26355811	4	37	theme	desorption	584:593	arg1	property					595:602	greater desorption property	576:602	greater desorption property	576:602	In order to increase the DNA extraction yield, a new surface with different functional groups which possess of greater desorption property is required.
26355811	3	38	theme	groups	350:355	arg1	force					325:329	adsorption force	314:329	adsorption force of SiOH functional groups	314:355	However, since adsorption force of SiOH functional groups is much greater than that of desorption force, the DNA extraction efficiency of silica surfaces is limited.
26355811	4	39	theme	new	514:516	arg1	surface					518:524	a new surface	512:524	a new surface with different functional groups which possess of greater desorption property	512:602	In order to increase the DNA extraction yield, a new surface with different functional groups which possess of greater desorption property is required.
26355811	5	40	theme	oxide	663:667	arg1	material					702:709	an alternative material	687:709	an alternative material for DNA adsorption and extraction	687:743	In this study, we proposed cellulose/graphite oxide (GO) composite as an alternative material for DNA adsorption and extraction.
26355811	5	40	theme	oxide	663:667	arg1	composite					674:682	cellulose/graphite oxide (GO) composite	644:682	cellulose/graphite oxide (GO) composite	644:682	In this study, we proposed cellulose/graphite oxide (GO) composite as an alternative material for DNA adsorption and extraction.
26355811	2	41	theme	DNA	268:270	arg1	adsorption					272:281	selective DNA adsorption	258:281	selective DNA adsorption	258:281	Up to date silica matrices has been widely applied as solid support for selective DNA adsorption and extraction.
26355811	4	42	theme	greater	576:582	arg1	property					595:602	greater desorption property	576:602	greater desorption property	576:602	In order to increase the DNA extraction yield, a new surface with different functional groups which possess of greater desorption property is required.
26355811	14	43	theme	elution	1546:1552	arg1	1.86					1574:1577	1.86	1574:1577	1.86	1574:1577	The absorbance ratios between 260 nm and 280 nm (A260/A280) of the DNA elution was demonstrated as 1.86, indicating the extracted DNA consisted of high purity.
26355811	14	43	theme	elution	1546:1552	arg1	ratios					1490:1495	The absorbance ratios	1475:1495	The absorbance ratios between 260 nm and 280 nm (A260/A280) of the DNA elution	1475:1552	The absorbance ratios between 260 nm and 280 nm (A260/A280) of the DNA elution was demonstrated as 1.86, indicating the extracted DNA consisted of high purity.
26355811	14	44	theme	high	1622:1625	arg1	purity					1627:1632	high purity	1622:1632	high purity	1622:1632	The absorbance ratios between 260 nm and 280 nm (A260/A280) of the DNA elution was demonstrated as 1.86, indicating the extracted DNA consisted of high purity.
26355811	13	45	theme	extraction	1379:1388	arg1	ng/μl					1423:1427	660.4 ng/μl	1417:1427	660.4 ng/μl	1417:1427	% GO, where the extraction efficiency was reported as 660.4 ng/μl when applying 2M GuHCl as the binding buffer.
26355811	13	45	theme	extraction	1379:1388	arg1	efficiency					1390:1399	the extraction efficiency	1375:1399	the extraction efficiency	1375:1399	% GO, where the extraction efficiency was reported as 660.4 ng/μl when applying 2M GuHCl as the binding buffer.
26355811	3	46	theme	DNA	408:410	arg1	efficiency					423:432	the DNA extraction efficiency	404:432	the DNA extraction efficiency of silica surfaces	404:451	However, since adsorption force of SiOH functional groups is much greater than that of desorption force, the DNA extraction efficiency of silica surfaces is limited.
26355811	0	47	theme	DNA	29:31	arg1	desorption					15:24	desorption	15:24	desorption	15:24	Adsorption and desorption of DNA tuned by hydroxyl groups in graphite oxides-based solid extraction material.
26355811	0	47	theme	DNA	29:31	arg1	Adsorption					0:9	Adsorption	0:9	Adsorption	0:9	Adsorption and desorption of DNA tuned by hydroxyl groups in graphite oxides-based solid extraction material.
26355811	6	48	theme	group	884:888	arg1	alkyl					846:850	alkyl	846:850	alkyl	846:850	GO/Cellulose composite provides the major adsorption and desorption of DNA by COH, which belongs to alkyl or phenol type of OH functional group.
26355811	6	48	theme	group	884:888	arg1	type					862:865	phenol type	855:865	phenol type	855:865	GO/Cellulose composite provides the major adsorption and desorption of DNA by COH, which belongs to alkyl or phenol type of OH functional group.
26355811	6	49	theme	DNA	817:819	arg1	desorption					803:812	desorption	803:812	desorption	803:812	GO/Cellulose composite provides the major adsorption and desorption of DNA by COH, which belongs to alkyl or phenol type of OH functional group.
26355811	6	49	theme	DNA	817:819	arg1	adsorption					788:797	major adsorption	782:797	major adsorption	782:797	GO/Cellulose composite provides the major adsorption and desorption of DNA by COH, which belongs to alkyl or phenol type of OH functional group.
26355811	12	50	theme	highest	1325:1331	arg1	yield					1333:1337	The highest yield	1321:1337	The highest yield	1321:1337	The highest yield was achieved at 4.15 wt.
26355811	3	51	theme	extraction	412:421	arg1	efficiency					423:432	the DNA extraction efficiency	404:432	the DNA extraction efficiency of silica surfaces	404:451	However, since adsorption force of SiOH functional groups is much greater than that of desorption force, the DNA extraction efficiency of silica surfaces is limited.
26355811	0	52	theme	hydroxyl	42:49	arg1	groups					51:56	hydroxyl groups	42:56	hydroxyl groups in graphite oxides-based solid extraction material	42:107	Adsorption and desorption of DNA tuned by hydroxyl groups in graphite oxides-based solid extraction material.
26355811	6	53	theme	functional	873:882	arg1	group					884:888	OH functional group	870:888	OH functional group	870:888	GO/Cellulose composite provides the major adsorption and desorption of DNA by COH, which belongs to alkyl or phenol type of OH functional group.
26355811	0	54	from	groups	51:56	arg1	material					100:107	graphite oxides-based solid extraction material	61:107	graphite oxides-based solid extraction material	61:107	Adsorption and desorption of DNA tuned by hydroxyl groups in graphite oxides-based solid extraction material.
26355811	2	55	theme	silica	197:202	arg1	matrices					204:211	date silica matrices	192:211	date silica matrices	192:211	Up to date silica matrices has been widely applied as solid support for selective DNA adsorption and extraction.
26355811	11	56	theme	extraction	1246:1255	arg1	yield					1257:1261	The extraction yield	1242:1261	The extraction yield of DNA	1242:1268	The extraction yield of DNA increased with increasing weight percentage of GO.
26355811	4	57	theme	extraction	494:503	arg1	yield					505:509	the DNA extraction yield	486:509	the DNA extraction yield	486:509	In order to increase the DNA extraction yield, a new surface with different functional groups which possess of greater desorption property is required.
26355811	7	58	theme	higher	985:990	arg1	desorption					992:1001	a higher desorption	983:1001	a higher desorption of DNA	983:1008	Compared to SiOH, COH is less polarized and reactive, therefore the composite might provide a higher desorption of DNA during the elution process.
26355811	2	59	theme	date	192:195	arg1	matrices					204:211	date silica matrices	192:211	date silica matrices	192:211	Up to date silica matrices has been widely applied as solid support for selective DNA adsorption and extraction.
26355811	14	60	theme	DNA	1542:1544	arg1	elution					1546:1552	the DNA elution	1538:1552	the DNA elution	1538:1552	The absorbance ratios between 260 nm and 280 nm (A260/A280) of the DNA elution was demonstrated as 1.86, indicating the extracted DNA consisted of high purity.
26355811	4	61	theme	DNA	490:492	arg1	yield					505:509	the DNA extraction yield	486:509	the DNA extraction yield	486:509	In order to increase the DNA extraction yield, a new surface with different functional groups which possess of greater desorption property is required.
26355811	3	62	theme	silica	437:442	arg1	surfaces					444:451	silica surfaces	437:451	silica surfaces	437:451	However, since adsorption force of SiOH functional groups is much greater than that of desorption force, the DNA extraction efficiency of silica surfaces is limited.
26355811	8	63	theme	spherical	1082:1090	arg1	structure					1092:1100	spherical structure	1082:1100	spherical structure	1082:1100	The GO/cellulose composite were prepared in spherical structure by mixing urea, cellulose, NaOH, Graphite oxide and water.
26355811	13	64	theme	binding	1459:1465	arg1	buffer					1467:1472	the binding buffer	1455:1472	the binding buffer	1455:1472	% GO, where the extraction efficiency was reported as 660.4 ng/μl when applying 2M GuHCl as the binding buffer.
26355811	13	64	theme	binding	1459:1465	arg1	GuHCl					1446:1450	2M GuHCl	1443:1450	2M GuHCl	1443:1450	% GO, where the extraction efficiency was reported as 660.4 ng/μl when applying 2M GuHCl as the binding buffer.
26355811	11	65	theme	GO	1317:1318	arg1	percentage					1303:1312	weight percentage	1296:1312	weight percentage of GO	1296:1318	The extraction yield of DNA increased with increasing weight percentage of GO.
26355811	1	66	theme	DNA	128:130	arg1	method					152:157	the most crucial method	135:157	the most crucial method used in molecular biology	135:183	The extraction of DNA is the most crucial method used in molecular biology.
26355811	1	66	theme	DNA	128:130	arg1	extraction					114:123	The extraction	110:123	The extraction of DNA	110:130	The extraction of DNA is the most crucial method used in molecular biology.
26355811	8	67	theme	GO/cellulose	1042:1053	arg1	composite					1055:1063	The GO/cellulose composite	1038:1063	The GO/cellulose composite	1038:1063	The GO/cellulose composite were prepared in spherical structure by mixing urea, cellulose, NaOH, Graphite oxide and water.
26355811	14	68	theme	extracted	1595:1603	arg1	DNA					1605:1607	the extracted DNA	1591:1607	the extracted DNA consisted of high purity	1591:1632	The absorbance ratios between 260 nm and 280 nm (A260/A280) of the DNA elution was demonstrated as 1.86, indicating the extracted DNA consisted of high purity.
26355811	5	69	theme	alternative	690:700	arg1	material					702:709	an alternative material	687:709	an alternative material for DNA adsorption and extraction	687:743	In this study, we proposed cellulose/graphite oxide (GO) composite as an alternative material for DNA adsorption and extraction.
26355811	5	69	theme	alternative	690:700	arg1	composite					674:682	cellulose/graphite oxide (GO) composite	644:682	cellulose/graphite oxide (GO) composite	644:682	In this study, we proposed cellulose/graphite oxide (GO) composite as an alternative material for DNA adsorption and extraction.
26355811	6	70	theme	major	782:786	arg1	adsorption					788:797	major adsorption	782:797	major adsorption	782:797	GO/Cellulose composite provides the major adsorption and desorption of DNA by COH, which belongs to alkyl or phenol type of OH functional group.
26355811	15	71	theme	simple	1693:1698	arg1	method					1700:1705	a simple method	1691:1705	a simple method for selective DNA extraction with high extraction efficiency of pure DNA	1691:1778	The results proved that GO/cellulose composite provides a simple method for selective DNA extraction with high extraction efficiency of pure DNA.
26355811	8	72	theme	Graphite	1135:1142	arg1	oxide					1144:1148	Graphite oxide	1135:1148	Graphite oxide	1135:1148	The GO/cellulose composite were prepared in spherical structure by mixing urea, cellulose, NaOH, Graphite oxide and water.
26355811	4	73	with	surface	518:524	arg1	groups					552:557	different functional groups	531:557	different functional groups which possess of greater desorption property	531:602	In order to increase the DNA extraction yield, a new surface with different functional groups which possess of greater desorption property is required.
26355811	15	74	theme	DNA	1776:1778	arg1	efficiency					1757:1766	high extraction efficiency	1741:1766	high extraction efficiency of pure DNA	1741:1778	The results proved that GO/cellulose composite provides a simple method for selective DNA extraction with high extraction efficiency of pure DNA.
26355811	0	75	theme	solid	83:87	arg1	material					100:107	graphite oxides-based solid extraction material	61:107	graphite oxides-based solid extraction material	61:107	Adsorption and desorption of DNA tuned by hydroxyl groups in graphite oxides-based solid extraction material.
25450832	0	0	theme	immunological	84:96	arg1	stimuli					98:104	immunological stimuli	84:104	immunological stimuli	84:104	Nanoparticle and polysaccharide conjugate: a potential candidate vaccine to improve immunological stimuli.
25450832	1	1	attach	isolated	130:137	arg2	polysaccharides					114:128	Active polysaccharides	107:128	Active polysaccharides isolated from various fungal sources	107:165	Active polysaccharides isolated from various fungal sources have been implicated to stimulate immune response against various pathogens as well as self anomalies such as cancer.
25450832	1	1	attach	isolated	130:137	arg1	sources					159:165	various fungal sources	144:165	various fungal sources	144:165	Active polysaccharides isolated from various fungal sources have been implicated to stimulate immune response against various pathogens as well as self anomalies such as cancer.
25450832	3	2	theme	reductive	563:571	arg1	amination					573:581	A Schiff base reductive amination	549:581	A Schiff base reductive amination reaction	549:590	A Schiff base reductive amination reaction occurred between the aldehyde group of the polysaccharide and the amine group of the nanoparticles in the presence of a strong reducing agent such as sodium cyanoborohydride to form a stable amide bond between the two conjugating molecules.
25450832	5	3	theme	soluble	1160:1166	arg1	form					1168:1171	the soluble form	1156:1171	the soluble form	1156:1171	This particulate form of the polysaccharide showed a marked escalation in the production of free radicals such as reactive oxygen and nitrogen species in murine macrophages as compared to the soluble form.
25450832	6	4	from	mice	1345:1348	arg1	proliferation					1264:1276	the proliferation	1260:1276	the proliferation of splenocytes in particulate or conjugated polysaccharide treated mice	1260:1348	Animal based experiments demonstrated a reduction in tumor volume and augmentation in the proliferation of splenocytes in particulate or conjugated polysaccharide treated mice.
25450832	2	5	with	ostreatus	391:399	arg1	nanoparticles					428:440	biocompatible ferrite nanoparticles	406:440	biocompatible ferrite nanoparticles	406:440	Therefore, the nuanced approach presented in our work was to blend polysaccharides derived from Pleurotus ostreatus with biocompatible ferrite nanoparticles and thereafter investigate the enhanced immune functionality of the polysaccharide-nanoparticle composite.
25450832	3	6	theme	sodium	742:747	arg1	cyanoborohydride					749:764	sodium cyanoborohydride	742:764	sodium cyanoborohydride to form a stable amide bond between the two conjugating molecules	742:830	A Schiff base reductive amination reaction occurred between the aldehyde group of the polysaccharide and the amine group of the nanoparticles in the presence of a strong reducing agent such as sodium cyanoborohydride to form a stable amide bond between the two conjugating molecules.
25450832	5	7	theme	murine	1122:1127	arg1	macrophages					1129:1139	murine macrophages	1122:1139	murine macrophages	1122:1139	This particulate form of the polysaccharide showed a marked escalation in the production of free radicals such as reactive oxygen and nitrogen species in murine macrophages as compared to the soluble form.
25450832	3	8	theme	amination	573:581	arg1	reaction					583:590	A Schiff base reductive amination reaction	549:590	A Schiff base reductive amination reaction	549:590	A Schiff base reductive amination reaction occurred between the aldehyde group of the polysaccharide and the amine group of the nanoparticles in the presence of a strong reducing agent such as sodium cyanoborohydride to form a stable amide bond between the two conjugating molecules.
25450832	3	9	from	presence	698:705	arg1	group					664:668	the amine group	654:668	the amine group of the nanoparticles in the presence of a strong reducing agent such as sodium cyanoborohydride to form a stable amide bond between the two conjugating molecules	654:830	A Schiff base reductive amination reaction occurred between the aldehyde group of the polysaccharide and the amine group of the nanoparticles in the presence of a strong reducing agent such as sodium cyanoborohydride to form a stable amide bond between the two conjugating molecules.
25450832	3	9	from	presence	698:705	arg1	nanoparticles					677:689	the nanoparticles	673:689	the nanoparticles in the presence of a strong reducing agent such as sodium cyanoborohydride to form a stable amide bond between the two conjugating molecules	673:830	A Schiff base reductive amination reaction occurred between the aldehyde group of the polysaccharide and the amine group of the nanoparticles in the presence of a strong reducing agent such as sodium cyanoborohydride to form a stable amide bond between the two conjugating molecules.
25450832	3	9	from	presence	698:705	arg1	group					622:626	the aldehyde group	609:626	the aldehyde group of the polysaccharide	609:648	A Schiff base reductive amination reaction occurred between the aldehyde group of the polysaccharide and the amine group of the nanoparticles in the presence of a strong reducing agent such as sodium cyanoborohydride to form a stable amide bond between the two conjugating molecules.
25450832	3	9	from	presence	698:705	arg1	polysaccharide					635:648	the polysaccharide	631:648	the polysaccharide	631:648	A Schiff base reductive amination reaction occurred between the aldehyde group of the polysaccharide and the amine group of the nanoparticles in the presence of a strong reducing agent such as sodium cyanoborohydride to form a stable amide bond between the two conjugating molecules.
25450832	3	9	from	presence	698:705	arg1	cyanoborohydride					749:764	sodium cyanoborohydride	742:764	sodium cyanoborohydride to form a stable amide bond between the two conjugating molecules	742:830	A Schiff base reductive amination reaction occurred between the aldehyde group of the polysaccharide and the amine group of the nanoparticles in the presence of a strong reducing agent such as sodium cyanoborohydride to form a stable amide bond between the two conjugating molecules.
25450832	2	10	attach	derived	368:374	arg1	ostreatus					391:399	Pleurotus ostreatus	381:399	Pleurotus ostreatus with biocompatible ferrite nanoparticles	381:440	Therefore, the nuanced approach presented in our work was to blend polysaccharides derived from Pleurotus ostreatus with biocompatible ferrite nanoparticles and thereafter investigate the enhanced immune functionality of the polysaccharide-nanoparticle composite.
25450832	2	10	attach	derived	368:374	arg2	polysaccharides					352:366	polysaccharides	352:366	polysaccharides derived from Pleurotus ostreatus with biocompatible ferrite nanoparticles	352:440	Therefore, the nuanced approach presented in our work was to blend polysaccharides derived from Pleurotus ostreatus with biocompatible ferrite nanoparticles and thereafter investigate the enhanced immune functionality of the polysaccharide-nanoparticle composite.
25450832	2	11	theme	nuanced	300:306	arg1	approach					308:315	the nuanced approach	296:315	the nuanced approach presented in our work	296:337	Therefore, the nuanced approach presented in our work was to blend polysaccharides derived from Pleurotus ostreatus with biocompatible ferrite nanoparticles and thereafter investigate the enhanced immune functionality of the polysaccharide-nanoparticle composite.
25450832	3	12	from	group	622:626	arg1	presence					698:705	the presence	694:705	the presence of a strong reducing agent	694:732	A Schiff base reductive amination reaction occurred between the aldehyde group of the polysaccharide and the amine group of the nanoparticles in the presence of a strong reducing agent such as sodium cyanoborohydride to form a stable amide bond between the two conjugating molecules.
25450832	7	13	theme	downstream	1523:1532	arg1	regulation					1546:1555	a cellular downstream mechanistic regulation	1512:1555	a cellular downstream mechanistic regulation behind the immunostimulative response	1512:1593	Furthermore, molecular signaling studies showed a high upregulation in p-p38 and p-MEK molecules in particulate polysaccharide treated RAW264.7 cells suggesting a cellular downstream mechanistic regulation behind the immunostimulative response.
25450832	3	14	theme	amide	783:787	arg1	bond					789:792	a stable amide bond	774:792	a stable amide bond between the two conjugating molecules	774:830	A Schiff base reductive amination reaction occurred between the aldehyde group of the polysaccharide and the amine group of the nanoparticles in the presence of a strong reducing agent such as sodium cyanoborohydride to form a stable amide bond between the two conjugating molecules.
25450832	4	15	dep	microscopy	924:933	arg1	measurements					954:965	measurements	954:965	measurements	954:965	The multifaceted conjugate was characterized by physiochemical techniques such as electron microscopy, FTIR, VSM and DLS measurements.
25450832	6	16	theme	splenocytes	1281:1291	arg1	proliferation					1264:1276	the proliferation	1260:1276	the proliferation of splenocytes in particulate or conjugated polysaccharide treated mice	1260:1348	Animal based experiments demonstrated a reduction in tumor volume and augmentation in the proliferation of splenocytes in particulate or conjugated polysaccharide treated mice.
25450832	7	17	theme	immunostimulative	1568:1584	arg1	response					1586:1593	the immunostimulative response	1564:1593	the immunostimulative response	1564:1593	Furthermore, molecular signaling studies showed a high upregulation in p-p38 and p-MEK molecules in particulate polysaccharide treated RAW264.7 cells suggesting a cellular downstream mechanistic regulation behind the immunostimulative response.
25450832	5	18	from	species	1111:1117	arg1	macrophages					1129:1139	murine macrophages	1122:1139	murine macrophages	1122:1139	This particulate form of the polysaccharide showed a marked escalation in the production of free radicals such as reactive oxygen and nitrogen species in murine macrophages as compared to the soluble form.
25450832	5	19	theme	polysaccharide	997:1010	arg1	form					985:988	This particulate form	968:988	This particulate form of the polysaccharide	968:1010	This particulate form of the polysaccharide showed a marked escalation in the production of free radicals such as reactive oxygen and nitrogen species in murine macrophages as compared to the soluble form.
25450832	7	20	theme	signaling	1374:1382	arg1	studies					1384:1390	molecular signaling studies	1364:1390	molecular signaling studies	1364:1390	Furthermore, molecular signaling studies showed a high upregulation in p-p38 and p-MEK molecules in particulate polysaccharide treated RAW264.7 cells suggesting a cellular downstream mechanistic regulation behind the immunostimulative response.
25450832	6	21	from	proliferation	1264:1276	arg1	mice					1345:1348	particulate or conjugated polysaccharide treated mice	1296:1348	particulate or conjugated polysaccharide treated mice	1296:1348	Animal based experiments demonstrated a reduction in tumor volume and augmentation in the proliferation of splenocytes in particulate or conjugated polysaccharide treated mice.
25450832	4	22	theme	multifaceted	837:848	arg1	conjugate					850:858	The multifaceted conjugate	833:858	The multifaceted conjugate	833:858	The multifaceted conjugate was characterized by physiochemical techniques such as electron microscopy, FTIR, VSM and DLS measurements.
25450832	3	23	from	nanoparticles	677:689	arg1	presence					698:705	the presence	694:705	the presence of a strong reducing agent	694:732	A Schiff base reductive amination reaction occurred between the aldehyde group of the polysaccharide and the amine group of the nanoparticles in the presence of a strong reducing agent such as sodium cyanoborohydride to form a stable amide bond between the two conjugating molecules.
25450832	2	24	theme	ferrite	420:426	arg1	nanoparticles					428:440	biocompatible ferrite nanoparticles	406:440	biocompatible ferrite nanoparticles	406:440	Therefore, the nuanced approach presented in our work was to blend polysaccharides derived from Pleurotus ostreatus with biocompatible ferrite nanoparticles and thereafter investigate the enhanced immune functionality of the polysaccharide-nanoparticle composite.
25450832	6	25	from	splenocytes	1281:1291	arg1	mice					1345:1348	particulate or conjugated polysaccharide treated mice	1296:1348	particulate or conjugated polysaccharide treated mice	1296:1348	Animal based experiments demonstrated a reduction in tumor volume and augmentation in the proliferation of splenocytes in particulate or conjugated polysaccharide treated mice.
25450832	4	26	theme	electron	915:922	arg1	microscopy					924:933	electron microscopy	915:933	electron microscopy	915:933	The multifaceted conjugate was characterized by physiochemical techniques such as electron microscopy, FTIR, VSM and DLS measurements.
25450832	7	27	from	molecules	1438:1446	arg1	p-p38					1422:1426	p-p38	1422:1426	p-p38	1422:1426	Furthermore, molecular signaling studies showed a high upregulation in p-p38 and p-MEK molecules in particulate polysaccharide treated RAW264.7 cells suggesting a cellular downstream mechanistic regulation behind the immunostimulative response.
25450832	7	27	from	molecules	1438:1446	arg1	cells					1495:1499	particulate polysaccharide treated RAW264.7 cells	1451:1499	particulate polysaccharide treated RAW264.7 cells	1451:1499	Furthermore, molecular signaling studies showed a high upregulation in p-p38 and p-MEK molecules in particulate polysaccharide treated RAW264.7 cells suggesting a cellular downstream mechanistic regulation behind the immunostimulative response.
25450832	7	28	theme	polysaccharide	1463:1476	arg1	cells					1495:1499	particulate polysaccharide treated RAW264.7 cells	1451:1499	particulate polysaccharide treated RAW264.7 cells	1451:1499	Furthermore, molecular signaling studies showed a high upregulation in p-p38 and p-MEK molecules in particulate polysaccharide treated RAW264.7 cells suggesting a cellular downstream mechanistic regulation behind the immunostimulative response.
25450832	2	29	theme	biocompatible	406:418	arg1	nanoparticles					428:440	biocompatible ferrite nanoparticles	406:440	biocompatible ferrite nanoparticles	406:440	Therefore, the nuanced approach presented in our work was to blend polysaccharides derived from Pleurotus ostreatus with biocompatible ferrite nanoparticles and thereafter investigate the enhanced immune functionality of the polysaccharide-nanoparticle composite.
25450832	5	30	theme	radicals	1065:1072	arg1	production					1046:1055	the production	1042:1055	the production of free radicals such as reactive oxygen and nitrogen species in murine macrophages	1042:1139	This particulate form of the polysaccharide showed a marked escalation in the production of free radicals such as reactive oxygen and nitrogen species in murine macrophages as compared to the soluble form.
25450832	3	31	theme	stable	776:781	arg1	bond					789:792	a stable amide bond	774:792	a stable amide bond between the two conjugating molecules	774:830	A Schiff base reductive amination reaction occurred between the aldehyde group of the polysaccharide and the amine group of the nanoparticles in the presence of a strong reducing agent such as sodium cyanoborohydride to form a stable amide bond between the two conjugating molecules.
25450832	3	32	theme	polysaccharide	635:648	arg1	group					664:668	the amine group	654:668	the amine group of the nanoparticles in the presence of a strong reducing agent such as sodium cyanoborohydride to form a stable amide bond between the two conjugating molecules	654:830	A Schiff base reductive amination reaction occurred between the aldehyde group of the polysaccharide and the amine group of the nanoparticles in the presence of a strong reducing agent such as sodium cyanoborohydride to form a stable amide bond between the two conjugating molecules.
25450832	3	32	theme	polysaccharide	635:648	arg1	nanoparticles					677:689	the nanoparticles	673:689	the nanoparticles in the presence of a strong reducing agent such as sodium cyanoborohydride to form a stable amide bond between the two conjugating molecules	673:830	A Schiff base reductive amination reaction occurred between the aldehyde group of the polysaccharide and the amine group of the nanoparticles in the presence of a strong reducing agent such as sodium cyanoborohydride to form a stable amide bond between the two conjugating molecules.
25450832	3	32	theme	polysaccharide	635:648	arg1	group					622:626	the aldehyde group	609:626	the aldehyde group of the polysaccharide	609:648	A Schiff base reductive amination reaction occurred between the aldehyde group of the polysaccharide and the amine group of the nanoparticles in the presence of a strong reducing agent such as sodium cyanoborohydride to form a stable amide bond between the two conjugating molecules.
25450832	3	32	theme	polysaccharide	635:648	arg1	polysaccharide					635:648	the polysaccharide	631:648	the polysaccharide	631:648	A Schiff base reductive amination reaction occurred between the aldehyde group of the polysaccharide and the amine group of the nanoparticles in the presence of a strong reducing agent such as sodium cyanoborohydride to form a stable amide bond between the two conjugating molecules.
25450832	3	32	theme	polysaccharide	635:648	arg1	cyanoborohydride					749:764	sodium cyanoborohydride	742:764	sodium cyanoborohydride to form a stable amide bond between the two conjugating molecules	742:830	A Schiff base reductive amination reaction occurred between the aldehyde group of the polysaccharide and the amine group of the nanoparticles in the presence of a strong reducing agent such as sodium cyanoborohydride to form a stable amide bond between the two conjugating molecules.
25450832	7	33	theme	molecular	1364:1372	arg1	studies					1384:1390	molecular signaling studies	1364:1390	molecular signaling studies	1364:1390	Furthermore, molecular signaling studies showed a high upregulation in p-p38 and p-MEK molecules in particulate polysaccharide treated RAW264.7 cells suggesting a cellular downstream mechanistic regulation behind the immunostimulative response.
25450832	1	34	theme	immune	201:206	arg1	response					208:215	immune response	201:215	immune response against various pathogens as well as self anomalies such as cancer	201:282	Active polysaccharides isolated from various fungal sources have been implicated to stimulate immune response against various pathogens as well as self anomalies such as cancer.
25450832	2	35	theme	immune	482:487	arg1	functionality					489:501	the enhanced immune functionality	469:501	the enhanced immune functionality of the polysaccharide-nanoparticle composite	469:546	Therefore, the nuanced approach presented in our work was to blend polysaccharides derived from Pleurotus ostreatus with biocompatible ferrite nanoparticles and thereafter investigate the enhanced immune functionality of the polysaccharide-nanoparticle composite.
25450832	6	36	theme	treated	1337:1343	arg1	mice					1345:1348	particulate or conjugated polysaccharide treated mice	1296:1348	particulate or conjugated polysaccharide treated mice	1296:1348	Animal based experiments demonstrated a reduction in tumor volume and augmentation in the proliferation of splenocytes in particulate or conjugated polysaccharide treated mice.
25450832	6	37	theme	tumor	1227:1231	arg1	volume					1233:1238	tumor volume	1227:1238	tumor volume	1227:1238	Animal based experiments demonstrated a reduction in tumor volume and augmentation in the proliferation of splenocytes in particulate or conjugated polysaccharide treated mice.
25450832	2	38	theme	enhanced	473:480	arg1	functionality					489:501	the enhanced immune functionality	469:501	the enhanced immune functionality of the polysaccharide-nanoparticle composite	469:546	Therefore, the nuanced approach presented in our work was to blend polysaccharides derived from Pleurotus ostreatus with biocompatible ferrite nanoparticles and thereafter investigate the enhanced immune functionality of the polysaccharide-nanoparticle composite.
25450832	3	39	theme	conjugating	810:820	arg1	molecules					822:830	the two conjugating molecules	802:830	the two conjugating molecules	802:830	A Schiff base reductive amination reaction occurred between the aldehyde group of the polysaccharide and the amine group of the nanoparticles in the presence of a strong reducing agent such as sodium cyanoborohydride to form a stable amide bond between the two conjugating molecules.
25450832	5	40	theme	nitrogen	1102:1109	arg1	species					1111:1117	reactive oxygen and nitrogen species	1082:1117	species	1111:1117	This particulate form of the polysaccharide showed a marked escalation in the production of free radicals such as reactive oxygen and nitrogen species in murine macrophages as compared to the soluble form.
25450832	1	41	theme	Active	107:112	arg1	polysaccharides					114:128	Active polysaccharides	107:128	Active polysaccharides isolated from various fungal sources	107:165	Active polysaccharides isolated from various fungal sources have been implicated to stimulate immune response against various pathogens as well as self anomalies such as cancer.
25450832	6	42	theme	polysaccharide	1322:1335	arg1	mice					1345:1348	particulate or conjugated polysaccharide treated mice	1296:1348	particulate or conjugated polysaccharide treated mice	1296:1348	Animal based experiments demonstrated a reduction in tumor volume and augmentation in the proliferation of splenocytes in particulate or conjugated polysaccharide treated mice.
25450832	7	43	theme	high	1401:1404	arg1	upregulation					1406:1417	a high upregulation	1399:1417	a high upregulation in p-p38	1399:1426	Furthermore, molecular signaling studies showed a high upregulation in p-p38 and p-MEK molecules in particulate polysaccharide treated RAW264.7 cells suggesting a cellular downstream mechanistic regulation behind the immunostimulative response.
25450832	6	44	theme	based	1181:1185	arg1	experiments					1187:1197	Animal based experiments	1174:1197	Animal based experiments	1174:1197	Animal based experiments demonstrated a reduction in tumor volume and augmentation in the proliferation of splenocytes in particulate or conjugated polysaccharide treated mice.
25450832	6	45	from	reduction	1214:1222	arg1	augmentation					1244:1255	augmentation	1244:1255	augmentation	1244:1255	Animal based experiments demonstrated a reduction in tumor volume and augmentation in the proliferation of splenocytes in particulate or conjugated polysaccharide treated mice.
25450832	6	45	from	reduction	1214:1222	arg1	proliferation					1264:1276	the proliferation	1260:1276	the proliferation of splenocytes in particulate or conjugated polysaccharide treated mice	1260:1348	Animal based experiments demonstrated a reduction in tumor volume and augmentation in the proliferation of splenocytes in particulate or conjugated polysaccharide treated mice.
25450832	6	45	from	reduction	1214:1222	arg1	volume					1233:1238	tumor volume	1227:1238	tumor volume	1227:1238	Animal based experiments demonstrated a reduction in tumor volume and augmentation in the proliferation of splenocytes in particulate or conjugated polysaccharide treated mice.
25450832	5	46	theme	particulate	973:983	arg1	form					985:988	This particulate form	968:988	This particulate form of the polysaccharide	968:1010	This particulate form of the polysaccharide showed a marked escalation in the production of free radicals such as reactive oxygen and nitrogen species in murine macrophages as compared to the soluble form.
25450832	3	47	from	group	664:668	arg1	presence					698:705	the presence	694:705	the presence of a strong reducing agent	694:732	A Schiff base reductive amination reaction occurred between the aldehyde group of the polysaccharide and the amine group of the nanoparticles in the presence of a strong reducing agent such as sodium cyanoborohydride to form a stable amide bond between the two conjugating molecules.
25450832	1	48	theme	various	225:231	arg1	cancer					277:282	cancer	277:282	cancer	277:282	Active polysaccharides isolated from various fungal sources have been implicated to stimulate immune response against various pathogens as well as self anomalies such as cancer.
25450832	1	48	theme	various	225:231	arg1	pathogens					233:241	various pathogens	225:241	various pathogens as well as self anomalies such as cancer	225:282	Active polysaccharides isolated from various fungal sources have been implicated to stimulate immune response against various pathogens as well as self anomalies such as cancer.
25450832	2	49	theme	composite	538:546	arg1	functionality					489:501	the enhanced immune functionality	469:501	the enhanced immune functionality of the polysaccharide-nanoparticle composite	469:546	Therefore, the nuanced approach presented in our work was to blend polysaccharides derived from Pleurotus ostreatus with biocompatible ferrite nanoparticles and thereafter investigate the enhanced immune functionality of the polysaccharide-nanoparticle composite.
25450832	7	50	dep	showed	1392:1397	arg1	suggesting					1501:1510	suggesting	1501:1510	showed a high upregulation in p-p38 and p-MEK molecules in particulate polysaccharide treated RAW264.7 cells suggesting a cellular downstream mechanistic regulation behind the immunostimulative response	1392:1593	Furthermore, molecular signaling studies showed a high upregulation in p-p38 and p-MEK molecules in particulate polysaccharide treated RAW264.7 cells suggesting a cellular downstream mechanistic regulation behind the immunostimulative response.
25450832	3	51	theme	strong	712:717	arg1	agent					728:732	a strong reducing agent	710:732	a strong reducing agent	710:732	A Schiff base reductive amination reaction occurred between the aldehyde group of the polysaccharide and the amine group of the nanoparticles in the presence of a strong reducing agent such as sodium cyanoborohydride to form a stable amide bond between the two conjugating molecules.
25450832	5	52	theme	reactive	1082:1089	arg1	oxygen					1091:1096	reactive oxygen and nitrogen species	1082:1117	oxygen	1091:1096	This particulate form of the polysaccharide showed a marked escalation in the production of free radicals such as reactive oxygen and nitrogen species in murine macrophages as compared to the soluble form.
25450832	5	53	from	oxygen	1091:1096	arg1	macrophages					1129:1139	murine macrophages	1122:1139	murine macrophages	1122:1139	This particulate form of the polysaccharide showed a marked escalation in the production of free radicals such as reactive oxygen and nitrogen species in murine macrophages as compared to the soluble form.
25450832	2	54	theme	polysaccharide-nanoparticle	510:536	arg1	composite					538:546	the polysaccharide-nanoparticle composite	506:546	the polysaccharide-nanoparticle composite	506:546	Therefore, the nuanced approach presented in our work was to blend polysaccharides derived from Pleurotus ostreatus with biocompatible ferrite nanoparticles and thereafter investigate the enhanced immune functionality of the polysaccharide-nanoparticle composite.
25450832	4	55	theme	physiochemical	881:894	arg1	techniques					896:905	physiochemical techniques	881:905	physiochemical techniques such as electron microscopy, FTIR, VSM and DLS measurements	881:965	The multifaceted conjugate was characterized by physiochemical techniques such as electron microscopy, FTIR, VSM and DLS measurements.
25450832	4	55	theme	physiochemical	881:894	arg1	FTIR					936:939	FTIR	936:939	FTIR	936:939	The multifaceted conjugate was characterized by physiochemical techniques such as electron microscopy, FTIR, VSM and DLS measurements.
25450832	4	55	theme	physiochemical	881:894	arg1	DLS					950:952	DLS	950:952	DLS	950:952	The multifaceted conjugate was characterized by physiochemical techniques such as electron microscopy, FTIR, VSM and DLS measurements.
25450832	4	55	theme	physiochemical	881:894	arg1	microscopy					924:933	electron microscopy	915:933	electron microscopy	915:933	The multifaceted conjugate was characterized by physiochemical techniques such as electron microscopy, FTIR, VSM and DLS measurements.
25450832	4	55	theme	physiochemical	881:894	arg1	VSM					942:944	VSM	942:944	VSM	942:944	The multifaceted conjugate was characterized by physiochemical techniques such as electron microscopy, FTIR, VSM and DLS measurements.
25450832	7	56	theme	RAW264.7	1486:1493	arg1	cells					1495:1499	particulate polysaccharide treated RAW264.7 cells	1451:1499	particulate polysaccharide treated RAW264.7 cells	1451:1499	Furthermore, molecular signaling studies showed a high upregulation in p-p38 and p-MEK molecules in particulate polysaccharide treated RAW264.7 cells suggesting a cellular downstream mechanistic regulation behind the immunostimulative response.
25450832	0	57	theme	candidate	55:63	arg1	vaccine					65:71	a potential candidate vaccine	43:71	a potential candidate vaccine	43:71	Nanoparticle and polysaccharide conjugate: a potential candidate vaccine to improve immunological stimuli.
25450832	7	58	theme	treated	1478:1484	arg1	cells					1495:1499	particulate polysaccharide treated RAW264.7 cells	1451:1499	particulate polysaccharide treated RAW264.7 cells	1451:1499	Furthermore, molecular signaling studies showed a high upregulation in p-p38 and p-MEK molecules in particulate polysaccharide treated RAW264.7 cells suggesting a cellular downstream mechanistic regulation behind the immunostimulative response.
25450832	6	59	theme	Animal	1174:1179	arg1	experiments					1187:1197	Animal based experiments	1174:1197	Animal based experiments	1174:1197	Animal based experiments demonstrated a reduction in tumor volume and augmentation in the proliferation of splenocytes in particulate or conjugated polysaccharide treated mice.
25450832	3	60	theme	Schiff	551:556	arg1	amination					573:581	A Schiff base reductive amination	549:581	A Schiff base reductive amination reaction	549:590	A Schiff base reductive amination reaction occurred between the aldehyde group of the polysaccharide and the amine group of the nanoparticles in the presence of a strong reducing agent such as sodium cyanoborohydride to form a stable amide bond between the two conjugating molecules.
25450832	0	61	theme	potential	45:53	arg1	vaccine					65:71	a potential candidate vaccine	43:71	a potential candidate vaccine	43:71	Nanoparticle and polysaccharide conjugate: a potential candidate vaccine to improve immunological stimuli.
25450832	3	62	theme	amine	658:662	arg1	group					664:668	the amine group	654:668	the amine group of the nanoparticles in the presence of a strong reducing agent such as sodium cyanoborohydride to form a stable amide bond between the two conjugating molecules	654:830	A Schiff base reductive amination reaction occurred between the aldehyde group of the polysaccharide and the amine group of the nanoparticles in the presence of a strong reducing agent such as sodium cyanoborohydride to form a stable amide bond between the two conjugating molecules.
25450832	3	62	theme	amine	658:662	arg1	nanoparticles					677:689	the nanoparticles	673:689	the nanoparticles in the presence of a strong reducing agent such as sodium cyanoborohydride to form a stable amide bond between the two conjugating molecules	673:830	A Schiff base reductive amination reaction occurred between the aldehyde group of the polysaccharide and the amine group of the nanoparticles in the presence of a strong reducing agent such as sodium cyanoborohydride to form a stable amide bond between the two conjugating molecules.
25450832	3	62	theme	amine	658:662	arg1	polysaccharide					635:648	the polysaccharide	631:648	the polysaccharide	631:648	A Schiff base reductive amination reaction occurred between the aldehyde group of the polysaccharide and the amine group of the nanoparticles in the presence of a strong reducing agent such as sodium cyanoborohydride to form a stable amide bond between the two conjugating molecules.
25450832	3	62	theme	amine	658:662	arg1	cyanoborohydride					749:764	sodium cyanoborohydride	742:764	sodium cyanoborohydride to form a stable amide bond between the two conjugating molecules	742:830	A Schiff base reductive amination reaction occurred between the aldehyde group of the polysaccharide and the amine group of the nanoparticles in the presence of a strong reducing agent such as sodium cyanoborohydride to form a stable amide bond between the two conjugating molecules.
25450832	7	63	theme	p-MEK	1432:1436	arg1	molecules					1438:1446	p-MEK molecules	1432:1446	p-MEK molecules in particulate polysaccharide treated RAW264.7 cells	1432:1499	Furthermore, molecular signaling studies showed a high upregulation in p-p38 and p-MEK molecules in particulate polysaccharide treated RAW264.7 cells suggesting a cellular downstream mechanistic regulation behind the immunostimulative response.
25450832	3	64	theme	base	558:561	arg1	amination					573:581	A Schiff base reductive amination	549:581	A Schiff base reductive amination reaction	549:590	A Schiff base reductive amination reaction occurred between the aldehyde group of the polysaccharide and the amine group of the nanoparticles in the presence of a strong reducing agent such as sodium cyanoborohydride to form a stable amide bond between the two conjugating molecules.
25450832	6	65	theme	particulate	1296:1306	arg1	mice					1345:1348	particulate or conjugated polysaccharide treated mice	1296:1348	particulate or conjugated polysaccharide treated mice	1296:1348	Animal based experiments demonstrated a reduction in tumor volume and augmentation in the proliferation of splenocytes in particulate or conjugated polysaccharide treated mice.
25450832	3	66	theme	nanoparticles	677:689	arg1	group					664:668	the amine group	654:668	the amine group of the nanoparticles in the presence of a strong reducing agent such as sodium cyanoborohydride to form a stable amide bond between the two conjugating molecules	654:830	A Schiff base reductive amination reaction occurred between the aldehyde group of the polysaccharide and the amine group of the nanoparticles in the presence of a strong reducing agent such as sodium cyanoborohydride to form a stable amide bond between the two conjugating molecules.
25450832	3	66	theme	nanoparticles	677:689	arg1	nanoparticles					677:689	the nanoparticles	673:689	the nanoparticles in the presence of a strong reducing agent such as sodium cyanoborohydride to form a stable amide bond between the two conjugating molecules	673:830	A Schiff base reductive amination reaction occurred between the aldehyde group of the polysaccharide and the amine group of the nanoparticles in the presence of a strong reducing agent such as sodium cyanoborohydride to form a stable amide bond between the two conjugating molecules.
25450832	3	66	theme	nanoparticles	677:689	arg1	group					622:626	the aldehyde group	609:626	the aldehyde group of the polysaccharide	609:648	A Schiff base reductive amination reaction occurred between the aldehyde group of the polysaccharide and the amine group of the nanoparticles in the presence of a strong reducing agent such as sodium cyanoborohydride to form a stable amide bond between the two conjugating molecules.
25450832	3	66	theme	nanoparticles	677:689	arg1	polysaccharide					635:648	the polysaccharide	631:648	the polysaccharide	631:648	A Schiff base reductive amination reaction occurred between the aldehyde group of the polysaccharide and the amine group of the nanoparticles in the presence of a strong reducing agent such as sodium cyanoborohydride to form a stable amide bond between the two conjugating molecules.
25450832	3	66	theme	nanoparticles	677:689	arg1	cyanoborohydride					749:764	sodium cyanoborohydride	742:764	sodium cyanoborohydride to form a stable amide bond between the two conjugating molecules	742:830	A Schiff base reductive amination reaction occurred between the aldehyde group of the polysaccharide and the amine group of the nanoparticles in the presence of a strong reducing agent such as sodium cyanoborohydride to form a stable amide bond between the two conjugating molecules.
25450832	7	67	theme	particulate	1451:1461	arg1	cells					1495:1499	particulate polysaccharide treated RAW264.7 cells	1451:1499	particulate polysaccharide treated RAW264.7 cells	1451:1499	Furthermore, molecular signaling studies showed a high upregulation in p-p38 and p-MEK molecules in particulate polysaccharide treated RAW264.7 cells suggesting a cellular downstream mechanistic regulation behind the immunostimulative response.
25450832	5	68	theme	free	1060:1063	arg1	oxygen					1091:1096	reactive oxygen and nitrogen species	1082:1117	oxygen	1091:1096	This particulate form of the polysaccharide showed a marked escalation in the production of free radicals such as reactive oxygen and nitrogen species in murine macrophages as compared to the soluble form.
25450832	5	68	theme	free	1060:1063	arg1	species					1111:1117	reactive oxygen and nitrogen species	1082:1117	species	1111:1117	This particulate form of the polysaccharide showed a marked escalation in the production of free radicals such as reactive oxygen and nitrogen species in murine macrophages as compared to the soluble form.
25450832	5	68	theme	free	1060:1063	arg1	radicals					1065:1072	free radicals	1060:1072	free radicals such as reactive oxygen and nitrogen species in murine macrophages	1060:1139	This particulate form of the polysaccharide showed a marked escalation in the production of free radicals such as reactive oxygen and nitrogen species in murine macrophages as compared to the soluble form.
25450832	6	69	theme	conjugated	1311:1320	arg1	mice					1345:1348	particulate or conjugated polysaccharide treated mice	1296:1348	particulate or conjugated polysaccharide treated mice	1296:1348	Animal based experiments demonstrated a reduction in tumor volume and augmentation in the proliferation of splenocytes in particulate or conjugated polysaccharide treated mice.
25450832	1	70	theme	various	144:150	arg1	sources					159:165	various fungal sources	144:165	various fungal sources	144:165	Active polysaccharides isolated from various fungal sources have been implicated to stimulate immune response against various pathogens as well as self anomalies such as cancer.
25450832	5	71	theme	marked	1021:1026	arg1	escalation					1028:1037	a marked escalation	1019:1037	a marked escalation in the production of free radicals such as reactive oxygen and nitrogen species in murine macrophages	1019:1139	This particulate form of the polysaccharide showed a marked escalation in the production of free radicals such as reactive oxygen and nitrogen species in murine macrophages as compared to the soluble form.
25450832	7	72	theme	cellular	1514:1521	arg1	regulation					1546:1555	a cellular downstream mechanistic regulation	1512:1555	a cellular downstream mechanistic regulation behind the immunostimulative response	1512:1593	Furthermore, molecular signaling studies showed a high upregulation in p-p38 and p-MEK molecules in particulate polysaccharide treated RAW264.7 cells suggesting a cellular downstream mechanistic regulation behind the immunostimulative response.
25450832	7	73	theme	mechanistic	1534:1544	arg1	regulation					1546:1555	a cellular downstream mechanistic regulation	1512:1555	a cellular downstream mechanistic regulation behind the immunostimulative response	1512:1593	Furthermore, molecular signaling studies showed a high upregulation in p-p38 and p-MEK molecules in particulate polysaccharide treated RAW264.7 cells suggesting a cellular downstream mechanistic regulation behind the immunostimulative response.
25450832	3	74	theme	reducing	719:726	arg1	agent					728:732	a strong reducing agent	710:732	a strong reducing agent	710:732	A Schiff base reductive amination reaction occurred between the aldehyde group of the polysaccharide and the amine group of the nanoparticles in the presence of a strong reducing agent such as sodium cyanoborohydride to form a stable amide bond between the two conjugating molecules.
25450832	3	75	theme	aldehyde	613:620	arg1	nanoparticles					677:689	the nanoparticles	673:689	the nanoparticles in the presence of a strong reducing agent such as sodium cyanoborohydride to form a stable amide bond between the two conjugating molecules	673:830	A Schiff base reductive amination reaction occurred between the aldehyde group of the polysaccharide and the amine group of the nanoparticles in the presence of a strong reducing agent such as sodium cyanoborohydride to form a stable amide bond between the two conjugating molecules.
25450832	3	75	theme	aldehyde	613:620	arg1	group					622:626	the aldehyde group	609:626	the aldehyde group of the polysaccharide	609:648	A Schiff base reductive amination reaction occurred between the aldehyde group of the polysaccharide and the amine group of the nanoparticles in the presence of a strong reducing agent such as sodium cyanoborohydride to form a stable amide bond between the two conjugating molecules.
25450832	3	75	theme	aldehyde	613:620	arg1	polysaccharide					635:648	the polysaccharide	631:648	the polysaccharide	631:648	A Schiff base reductive amination reaction occurred between the aldehyde group of the polysaccharide and the amine group of the nanoparticles in the presence of a strong reducing agent such as sodium cyanoborohydride to form a stable amide bond between the two conjugating molecules.
25450832	3	75	theme	aldehyde	613:620	arg1	cyanoborohydride					749:764	sodium cyanoborohydride	742:764	sodium cyanoborohydride to form a stable amide bond between the two conjugating molecules	742:830	A Schiff base reductive amination reaction occurred between the aldehyde group of the polysaccharide and the amine group of the nanoparticles in the presence of a strong reducing agent such as sodium cyanoborohydride to form a stable amide bond between the two conjugating molecules.
25450832	1	76	theme	fungal	152:157	arg1	sources					159:165	various fungal sources	144:165	various fungal sources	144:165	Active polysaccharides isolated from various fungal sources have been implicated to stimulate immune response against various pathogens as well as self anomalies such as cancer.
25450832	3	77	theme	agent	728:732	arg1	presence					698:705	the presence	694:705	the presence of a strong reducing agent	694:732	A Schiff base reductive amination reaction occurred between the aldehyde group of the polysaccharide and the amine group of the nanoparticles in the presence of a strong reducing agent such as sodium cyanoborohydride to form a stable amide bond between the two conjugating molecules.
25450832	7	78	from	upregulation	1406:1417	arg1	p-p38					1422:1426	p-p38	1422:1426	p-p38	1422:1426	Furthermore, molecular signaling studies showed a high upregulation in p-p38 and p-MEK molecules in particulate polysaccharide treated RAW264.7 cells suggesting a cellular downstream mechanistic regulation behind the immunostimulative response.
25450832	7	78	from	upregulation	1406:1417	arg1	cells					1495:1499	particulate polysaccharide treated RAW264.7 cells	1451:1499	particulate polysaccharide treated RAW264.7 cells	1451:1499	Furthermore, molecular signaling studies showed a high upregulation in p-p38 and p-MEK molecules in particulate polysaccharide treated RAW264.7 cells suggesting a cellular downstream mechanistic regulation behind the immunostimulative response.
25450832	2	79	theme	Pleurotus	381:389	arg1	ostreatus					391:399	Pleurotus ostreatus	381:399	Pleurotus ostreatus with biocompatible ferrite nanoparticles	381:440	Therefore, the nuanced approach presented in our work was to blend polysaccharides derived from Pleurotus ostreatus with biocompatible ferrite nanoparticles and thereafter investigate the enhanced immune functionality of the polysaccharide-nanoparticle composite.
25450832	5	80	from	escalation	1028:1037	arg1	production					1046:1055	the production	1042:1055	the production of free radicals such as reactive oxygen and nitrogen species in murine macrophages	1042:1139	This particulate form of the polysaccharide showed a marked escalation in the production of free radicals such as reactive oxygen and nitrogen species in murine macrophages as compared to the soluble form.
25450832	1	81	theme	self	254:257	arg1	anomalies					259:267	self anomalies	254:267	various pathogens as well as self anomalies such as cancer	225:282	Active polysaccharides isolated from various fungal sources have been implicated to stimulate immune response against various pathogens as well as self anomalies such as cancer.
25450832	1	81	theme	self	254:257	arg1	cancer					277:282	cancer	277:282	cancer	277:282	Active polysaccharides isolated from various fungal sources have been implicated to stimulate immune response against various pathogens as well as self anomalies such as cancer.
25450832	0	82	dep	Nanoparticle	0:11	arg1	conjugate					32:40	conjugate	32:40	conjugate	32:40	Nanoparticle and polysaccharide conjugate: a potential candidate vaccine to improve immunological stimuli.
24507324	6	0	theme	HA/CS/PVA	836:844	arg1	hydrogels					846:854	HA/CS/PVA hydrogels	836:854	HA/CS/PVA hydrogels	836:854	HA/CS/PVA hydrogels showed an 85-88% degree of gelation under 15 kGy radiation.
24507324	5	1	dep	skin	818:821	arg1	substitutes					823:833	substitutes	823:833	substitutes	823:833	The HA/CS/PVA hydrogels with various compositions were prepared by using γ-ray irradiation technique and their physicochemical properties were investigated to evaluate the feasibility of their use as artificial skin substitutes.
24507324	6	2	theme	kGy	901:903	arg1	radiation					905:913	15 kGy radiation	898:913	15 kGy radiation	898:913	HA/CS/PVA hydrogels showed an 85-88% degree of gelation under 15 kGy radiation.
24507324	5	3	theme	HA/CS/PVA	611:619	arg1	hydrogels					621:629	The HA/CS/PVA hydrogels	607:629	The HA/CS/PVA hydrogels with various compositions	607:655	The HA/CS/PVA hydrogels with various compositions were prepared by using γ-ray irradiation technique and their physicochemical properties were investigated to evaluate the feasibility of their use as artificial skin substitutes.
24507324	1	4	theme	tissue	194:199	arg1	engineering					201:211	skin tissue engineering	189:211	skin tissue engineering	189:211	Gamma-ray irradiation of novel hydrogels was used to develop a biocompatible hydrogel system for skin tissue engineering.
24507324	12	5	from	growth	1551:1556	arg1	hydrogels					1571:1579	HA/CS/PVA hydrogels	1561:1579	HA/CS/PVA hydrogels	1561:1579	The HaCaT growth in HA/CS/PVA hydrogels gradually increased as a function of culture time.
24507324	8	6	with	Hydrogels	1032:1040	arg1	concentrations					1054:1067	higher concentrations	1047:1067	higher concentrations of hyaluronidase solution and HA/CS content	1047:1111	Hydrogels with higher concentrations of hyaluronidase solution and HA/CS content had proportionally higher enzymatic degradation rates.
24507324	13	7	dep	viability	1713:1721	arg1	%					1711:1711	%	1711:1711	%	1711:1711	After 7 days, the HaCaT cells in all HA/CA/PVA hydrogels exhibited more than 80% viability compared to the control group HaCaT culture on a culture plate.
24507324	2	8	theme	vinyl	362:366	arg1	poly					357:360	poly	357:360	poly(vinyl alcohol) (PVA)	357:381	These novel hydrogels are composed of natural polymers including hyaluronic acid (HA) and chondroitin sulfate (CS), and the synthetic polymer, poly(vinyl alcohol) (PVA).
24507324	2	8	theme	vinyl	362:366	arg1	alcohol					368:374	vinyl alcohol	362:374	vinyl alcohol	362:374	These novel hydrogels are composed of natural polymers including hyaluronic acid (HA) and chondroitin sulfate (CS), and the synthetic polymer, poly(vinyl alcohol) (PVA).
24507324	7	9	dep	%	962:962	arg1	90					960:961	90	960:961	90	960:961	All HA/CS/PVA hydrogels exhibited more than 90% water content and reached an equilibrium swelling state within 24h.
24507324	5	10	theme	physicochemical	718:732	arg1	properties					734:743	their physicochemical properties	712:743	their physicochemical properties	712:743	The HA/CS/PVA hydrogels with various compositions were prepared by using γ-ray irradiation technique and their physicochemical properties were investigated to evaluate the feasibility of their use as artificial skin substitutes.
24507324	9	11	theme	properties	1280:1289	arg1	composition					1243:1253	the composition	1239:1253	the composition of the hydrogel and drug properties	1239:1289	The drug release behaviors from HA/CS/PVA hydrogels were influenced by the composition of the hydrogel and drug properties.
24507324	2	12	theme	natural	252:258	arg1	polymers					260:267	natural polymers	252:267	natural polymers including hyaluronic acid (HA) and chondroitin sulfate (CS)	252:327	These novel hydrogels are composed of natural polymers including hyaluronic acid (HA) and chondroitin sulfate (CS), and the synthetic polymer, poly(vinyl alcohol) (PVA).
24507324	2	12	theme	natural	252:258	arg1	acid					290:293	hyaluronic acid	279:293	hyaluronic acid (HA)	279:298	These novel hydrogels are composed of natural polymers including hyaluronic acid (HA) and chondroitin sulfate (CS), and the synthetic polymer, poly(vinyl alcohol) (PVA).
24507324	2	12	theme	natural	252:258	arg1	sulfate					316:322	chondroitin sulfate	304:322	chondroitin sulfate (CS)	304:327	These novel hydrogels are composed of natural polymers including hyaluronic acid (HA) and chondroitin sulfate (CS), and the synthetic polymer, poly(vinyl alcohol) (PVA).
24507324	12	13	theme	time	1626:1629	arg1	function					1606:1613	a function	1604:1613	a function of culture time	1604:1629	The HaCaT growth in HA/CS/PVA hydrogels gradually increased as a function of culture time.
24507324	5	14	theme	irradiation	686:696	arg1	technique					698:706	γ-ray irradiation technique	680:706	γ-ray irradiation technique	680:706	The HA/CS/PVA hydrogels with various compositions were prepared by using γ-ray irradiation technique and their physicochemical properties were investigated to evaluate the feasibility of their use as artificial skin substitutes.
24507324	7	15	theme	equilibrium	993:1003	arg1	state					1014:1018	an equilibrium swelling state	990:1018	an equilibrium swelling state	990:1018	All HA/CS/PVA hydrogels exhibited more than 90% water content and reached an equilibrium swelling state within 24h.
24507324	1	16	theme	hydrogel	169:176	arg1	system					178:183	a biocompatible hydrogel system	153:183	a biocompatible hydrogel system for skin tissue engineering	153:211	Gamma-ray irradiation of novel hydrogels was used to develop a biocompatible hydrogel system for skin tissue engineering.
24507324	10	17	theme	human	1304:1308	arg1	culture					1331:1337	human keratinocyte (HaCaT) culture	1304:1337	human keratinocyte (HaCaT) culture	1304:1337	Exposure of human keratinocyte (HaCaT) culture to the extracts of HA/CS/PVA hydrogels did not significantly affect the cell viability.
24507324	11	18	dep	%	1523:1523	arg1	92					1521:1522	92	1521:1522	92	1521:1522	All HaCaT cell cultures exposed to the extracts of HA/CS/PVA hydrogels exhibited greater than 92% cell viability.
24507324	8	19	theme	higher	1047:1052	arg1	concentrations					1054:1067	higher concentrations	1047:1067	higher concentrations of hyaluronidase solution and HA/CS content	1047:1111	Hydrogels with higher concentrations of hyaluronidase solution and HA/CS content had proportionally higher enzymatic degradation rates.
24507324	11	20	dep	viability	1530:1538	arg1	%					1523:1523	%	1523:1523	%	1523:1523	All HaCaT cell cultures exposed to the extracts of HA/CS/PVA hydrogels exhibited greater than 92% cell viability.
24507324	11	21	theme	HA/CS/PVA	1478:1486	arg1	hydrogels					1488:1496	HA/CS/PVA hydrogels	1478:1496	HA/CS/PVA hydrogels	1478:1496	All HaCaT cell cultures exposed to the extracts of HA/CS/PVA hydrogels exhibited greater than 92% cell viability.
24507324	3	22	theme	γ-ray	388:392	arg1	method					406:411	The γ-ray irradiation method	384:411	The γ-ray irradiation method	384:411	The γ-ray irradiation method has advantages, such as relatively simple manipulation without need of any extra reagents for polymerization and cross-linking.
24507324	0	23	theme	Hyaluronic	0:9	arg1	acid/chondroitin					11:26	Hyaluronic acid/chondroitin	0:26	Hyaluronic acid/chondroitin	0:26	Hyaluronic acid/chondroitin sulfate-based hydrogel prepared by gamma irradiation technique.
24507324	8	24	theme	solution	1086:1093	arg1	concentrations					1054:1067	higher concentrations	1047:1067	higher concentrations of hyaluronidase solution and HA/CS content	1047:1111	Hydrogels with higher concentrations of hyaluronidase solution and HA/CS content had proportionally higher enzymatic degradation rates.
24507324	1	25	theme	novel	117:121	arg1	hydrogels					123:131	novel hydrogels	117:131	novel hydrogels	117:131	Gamma-ray irradiation of novel hydrogels was used to develop a biocompatible hydrogel system for skin tissue engineering.
24507324	8	26	theme	HA/CS	1099:1103	arg1	content					1105:1111	HA/CS content	1099:1111	HA/CS content	1099:1111	Hydrogels with higher concentrations of hyaluronidase solution and HA/CS content had proportionally higher enzymatic degradation rates.
24507324	6	27	theme	gelation	883:890	arg1	degree					873:878	an 85-88% degree	863:878	an 85-88% degree of gelation	863:890	HA/CS/PVA hydrogels showed an 85-88% degree of gelation under 15 kGy radiation.
24507324	5	28	theme	use	800:802	arg1	feasibility					779:789	the feasibility	775:789	the feasibility of their use	775:802	The HA/CS/PVA hydrogels with various compositions were prepared by using γ-ray irradiation technique and their physicochemical properties were investigated to evaluate the feasibility of their use as artificial skin substitutes.
24507324	2	29	theme	synthetic	338:346	arg1	poly					357:360	poly	357:360	poly(vinyl alcohol) (PVA)	357:381	These novel hydrogels are composed of natural polymers including hyaluronic acid (HA) and chondroitin sulfate (CS), and the synthetic polymer, poly(vinyl alcohol) (PVA).
24507324	2	29	theme	synthetic	338:346	arg1	polymer					348:354	the synthetic polymer	334:354	the synthetic polymer	334:354	These novel hydrogels are composed of natural polymers including hyaluronic acid (HA) and chondroitin sulfate (CS), and the synthetic polymer, poly(vinyl alcohol) (PVA).
24507324	5	30	theme	artificial	807:816	arg1	skin					818:821	artificial skin substitutes	807:833	artificial skin substitutes	807:833	The HA/CS/PVA hydrogels with various compositions were prepared by using γ-ray irradiation technique and their physicochemical properties were investigated to evaluate the feasibility of their use as artificial skin substitutes.
24507324	8	31	theme	higher	1132:1137	arg1	rates					1161:1165	proportionally higher enzymatic degradation rates	1117:1165	proportionally higher enzymatic degradation rates	1117:1165	Hydrogels with higher concentrations of hyaluronidase solution and HA/CS content had proportionally higher enzymatic degradation rates.
24507324	6	32	theme	85-88	866:870	arg1	%					871:871	%	871:871	%	871:871	HA/CS/PVA hydrogels showed an 85-88% degree of gelation under 15 kGy radiation.
24507324	10	33	theme	HA/CS/PVA	1358:1366	arg1	hydrogels					1368:1376	HA/CS/PVA hydrogels	1358:1376	HA/CS/PVA hydrogels	1358:1376	Exposure of human keratinocyte (HaCaT) culture to the extracts of HA/CS/PVA hydrogels did not significantly affect the cell viability.
24507324	13	34	from	cells	1656:1660	arg1	hydrogels					1679:1687	all HA/CA/PVA hydrogels	1665:1687	all HA/CA/PVA hydrogels	1665:1687	After 7 days, the HaCaT cells in all HA/CA/PVA hydrogels exhibited more than 80% viability compared to the control group HaCaT culture on a culture plate.
24507324	8	35	theme	degradation	1149:1159	arg1	rates					1161:1165	proportionally higher enzymatic degradation rates	1117:1165	proportionally higher enzymatic degradation rates	1117:1165	Hydrogels with higher concentrations of hyaluronidase solution and HA/CS content had proportionally higher enzymatic degradation rates.
24507324	8	36	contain	had	1113:1115	arg2	rates					1161:1165	proportionally higher enzymatic degradation rates	1117:1165	proportionally higher enzymatic degradation rates	1117:1165	Hydrogels with higher concentrations of hyaluronidase solution and HA/CS content had proportionally higher enzymatic degradation rates.
24507324	8	36	contain	had	1113:1115	arg1	Hydrogels					1032:1040	Hydrogels	1032:1040	Hydrogels with higher concentrations of hyaluronidase solution and HA/CS content	1032:1111	Hydrogels with higher concentrations of hyaluronidase solution and HA/CS content had proportionally higher enzymatic degradation rates.
24507324	5	37	theme	various	636:642	arg1	compositions					644:655	various compositions	636:655	various compositions	636:655	The HA/CS/PVA hydrogels with various compositions were prepared by using γ-ray irradiation technique and their physicochemical properties were investigated to evaluate the feasibility of their use as artificial skin substitutes.
24507324	6	38	theme	%	871:871	arg1	degree					873:878	an 85-88% degree	863:878	an 85-88% degree of gelation	863:890	HA/CS/PVA hydrogels showed an 85-88% degree of gelation under 15 kGy radiation.
24507324	4	39	theme	HA	556:557	arg1	derivatives					566:576	HA and CS derivatives	556:576	HA and CS derivatives with polymerizable residues	556:604	We synthesized HA and CS derivatives with polymerizable residues.
24507324	2	40	theme	chondroitin	304:314	arg1	CS					325:326	CS	325:326	CS	325:326	These novel hydrogels are composed of natural polymers including hyaluronic acid (HA) and chondroitin sulfate (CS), and the synthetic polymer, poly(vinyl alcohol) (PVA).
24507324	2	40	theme	chondroitin	304:314	arg1	sulfate					316:322	chondroitin sulfate	304:322	chondroitin sulfate (CS)	304:327	These novel hydrogels are composed of natural polymers including hyaluronic acid (HA) and chondroitin sulfate (CS), and the synthetic polymer, poly(vinyl alcohol) (PVA).
24507324	4	41	theme	CS	563:564	arg1	derivatives					566:576	HA and CS derivatives	556:576	HA and CS derivatives with polymerizable residues	556:604	We synthesized HA and CS derivatives with polymerizable residues.
24507324	11	42	theme	cell	1437:1440	arg1	cultures					1442:1449	All HaCaT cell cultures	1427:1449	All HaCaT cell cultures exposed to the extracts of HA/CS/PVA hydrogels	1427:1496	All HaCaT cell cultures exposed to the extracts of HA/CS/PVA hydrogels exhibited greater than 92% cell viability.
24507324	3	43	theme	reagents	494:501	arg1	need					476:479	need	476:479	need of any extra reagents for polymerization and cross-linking	476:538	The γ-ray irradiation method has advantages, such as relatively simple manipulation without need of any extra reagents for polymerization and cross-linking.
24507324	1	44	theme	skin	189:192	arg1	engineering					201:211	skin tissue engineering	189:211	skin tissue engineering	189:211	Gamma-ray irradiation of novel hydrogels was used to develop a biocompatible hydrogel system for skin tissue engineering.
24507324	0	45	theme	gamma	63:67	arg1	technique					81:89	gamma irradiation technique	63:89	gamma irradiation technique	63:89	Hyaluronic acid/chondroitin sulfate-based hydrogel prepared by gamma irradiation technique.
24507324	9	46	from	hydrogels	1210:1218	arg1	behaviors					1185:1193	The drug release behaviors	1168:1193	The drug release behaviors from HA/CS/PVA hydrogels	1168:1218	The drug release behaviors from HA/CS/PVA hydrogels were influenced by the composition of the hydrogel and drug properties.
24507324	13	47	theme	culture	1772:1778	arg1	plate					1780:1784	a culture plate	1770:1784	a culture plate	1770:1784	After 7 days, the HaCaT cells in all HA/CA/PVA hydrogels exhibited more than 80% viability compared to the control group HaCaT culture on a culture plate.
24507324	9	48	theme	hydrogel	1262:1269	arg1	properties					1280:1289	the hydrogel and drug properties	1258:1289	properties	1280:1289	The drug release behaviors from HA/CS/PVA hydrogels were influenced by the composition of the hydrogel and drug properties.
24507324	9	49	theme	drug	1172:1175	arg1	behaviors					1185:1193	The drug release behaviors	1168:1193	The drug release behaviors from HA/CS/PVA hydrogels	1168:1218	The drug release behaviors from HA/CS/PVA hydrogels were influenced by the composition of the hydrogel and drug properties.
24507324	1	50	used	used	137:140	arg2	irradiation					102:112	Gamma-ray irradiation	92:112	Gamma-ray irradiation of novel hydrogels	92:131	Gamma-ray irradiation of novel hydrogels was used to develop a biocompatible hydrogel system for skin tissue engineering.
24507324	13	51	theme	control	1739:1745	arg1	culture					1759:1765	the control group HaCaT culture	1735:1765	the control group HaCaT culture on a culture plate	1735:1784	After 7 days, the HaCaT cells in all HA/CA/PVA hydrogels exhibited more than 80% viability compared to the control group HaCaT culture on a culture plate.
24507324	9	52	theme	drug	1275:1278	arg1	properties					1280:1289	the hydrogel and drug properties	1258:1289	properties	1280:1289	The drug release behaviors from HA/CS/PVA hydrogels were influenced by the composition of the hydrogel and drug properties.
24507324	13	53	dep	%	1711:1711	arg1	80					1709:1710	80	1709:1710	80	1709:1710	After 7 days, the HaCaT cells in all HA/CA/PVA hydrogels exhibited more than 80% viability compared to the control group HaCaT culture on a culture plate.
24507324	12	54	theme	culture	1618:1624	arg1	time					1626:1629	culture time	1618:1629	culture time	1618:1629	The HaCaT growth in HA/CS/PVA hydrogels gradually increased as a function of culture time.
24507324	13	55	theme	HaCaT	1753:1757	arg1	culture					1759:1765	the control group HaCaT culture	1735:1765	the control group HaCaT culture on a culture plate	1735:1784	After 7 days, the HaCaT cells in all HA/CA/PVA hydrogels exhibited more than 80% viability compared to the control group HaCaT culture on a culture plate.
24507324	11	56	theme	cell	1525:1528	arg1	viability					1530:1538	greater than 92% cell viability	1508:1538	greater than 92% cell viability	1508:1538	All HaCaT cell cultures exposed to the extracts of HA/CS/PVA hydrogels exhibited greater than 92% cell viability.
24507324	5	57	theme	γ-ray	680:684	arg1	technique					698:706	γ-ray irradiation technique	680:706	γ-ray irradiation technique	680:706	The HA/CS/PVA hydrogels with various compositions were prepared by using γ-ray irradiation technique and their physicochemical properties were investigated to evaluate the feasibility of their use as artificial skin substitutes.
24507324	4	58	with	derivatives	566:576	arg1	residues					597:604	polymerizable residues	583:604	polymerizable residues	583:604	We synthesized HA and CS derivatives with polymerizable residues.
24507324	9	59	theme	release	1177:1183	arg1	behaviors					1185:1193	The drug release behaviors	1168:1193	The drug release behaviors from HA/CS/PVA hydrogels	1168:1218	The drug release behaviors from HA/CS/PVA hydrogels were influenced by the composition of the hydrogel and drug properties.
24507324	3	60	contain	has	413:415	arg1	method					406:411	The γ-ray irradiation method	384:411	The γ-ray irradiation method	384:411	The γ-ray irradiation method has advantages, such as relatively simple manipulation without need of any extra reagents for polymerization and cross-linking.
24507324	3	60	contain	has	413:415	arg2	advantages					417:426	advantages	417:426	advantages	417:426	The γ-ray irradiation method has advantages, such as relatively simple manipulation without need of any extra reagents for polymerization and cross-linking.
24507324	3	60	contain	has	413:415	arg2	manipulation					455:466	relatively simple manipulation	437:466	relatively simple manipulation without need of any extra reagents for polymerization and cross-linking	437:538	The γ-ray irradiation method has advantages, such as relatively simple manipulation without need of any extra reagents for polymerization and cross-linking.
24507324	7	61	dep	content	970:976	arg1	%					962:962	%	962:962	%	962:962	All HA/CS/PVA hydrogels exhibited more than 90% water content and reached an equilibrium swelling state within 24h.
24507324	2	62	theme	hyaluronic	279:288	arg1	HA					296:297	HA	296:297	HA	296:297	These novel hydrogels are composed of natural polymers including hyaluronic acid (HA) and chondroitin sulfate (CS), and the synthetic polymer, poly(vinyl alcohol) (PVA).
24507324	2	62	theme	hyaluronic	279:288	arg1	acid					290:293	hyaluronic acid	279:293	hyaluronic acid (HA)	279:298	These novel hydrogels are composed of natural polymers including hyaluronic acid (HA) and chondroitin sulfate (CS), and the synthetic polymer, poly(vinyl alcohol) (PVA).
24507324	7	63	theme	swelling	1005:1012	arg1	state					1014:1018	an equilibrium swelling state	990:1018	an equilibrium swelling state	990:1018	All HA/CS/PVA hydrogels exhibited more than 90% water content and reached an equilibrium swelling state within 24h.
24507324	1	64	theme	biocompatible	155:167	arg1	system					178:183	a biocompatible hydrogel system	153:183	a biocompatible hydrogel system for skin tissue engineering	153:211	Gamma-ray irradiation of novel hydrogels was used to develop a biocompatible hydrogel system for skin tissue engineering.
24507324	10	65	theme	keratinocyte	1310:1321	arg1	culture					1331:1337	human keratinocyte (HaCaT) culture	1304:1337	human keratinocyte (HaCaT) culture	1304:1337	Exposure of human keratinocyte (HaCaT) culture to the extracts of HA/CS/PVA hydrogels did not significantly affect the cell viability.
24507324	13	66	from	culture	1759:1765	arg1	plate					1780:1784	a culture plate	1770:1784	a culture plate	1770:1784	After 7 days, the HaCaT cells in all HA/CA/PVA hydrogels exhibited more than 80% viability compared to the control group HaCaT culture on a culture plate.
24507324	12	67	theme	HA/CS/PVA	1561:1569	arg1	hydrogels					1571:1579	HA/CS/PVA hydrogels	1561:1579	HA/CS/PVA hydrogels	1561:1579	The HaCaT growth in HA/CS/PVA hydrogels gradually increased as a function of culture time.
24507324	10	68	theme	culture	1331:1337	arg1	Exposure					1292:1299	Exposure	1292:1299	Exposure of human keratinocyte (HaCaT) culture to the extracts of HA/CS/PVA hydrogels	1292:1376	Exposure of human keratinocyte (HaCaT) culture to the extracts of HA/CS/PVA hydrogels did not significantly affect the cell viability.
24507324	8	69	theme	hyaluronidase	1072:1084	arg1	solution					1086:1093	hyaluronidase solution	1072:1093	hyaluronidase solution	1072:1093	Hydrogels with higher concentrations of hyaluronidase solution and HA/CS content had proportionally higher enzymatic degradation rates.
24507324	7	70	theme	water	964:968	arg1	content					970:976	more than 90% water content	950:976	more than 90% water content	950:976	All HA/CS/PVA hydrogels exhibited more than 90% water content and reached an equilibrium swelling state within 24h.
24507324	1	71	theme	Gamma-ray	92:100	arg1	irradiation					102:112	Gamma-ray irradiation	92:112	Gamma-ray irradiation of novel hydrogels	92:131	Gamma-ray irradiation of novel hydrogels was used to develop a biocompatible hydrogel system for skin tissue engineering.
24507324	10	72	theme	HaCaT	1324:1328	arg1	culture					1331:1337	human keratinocyte (HaCaT) culture	1304:1337	human keratinocyte (HaCaT) culture	1304:1337	Exposure of human keratinocyte (HaCaT) culture to the extracts of HA/CS/PVA hydrogels did not significantly affect the cell viability.
24507324	3	73	theme	irradiation	394:404	arg1	method					406:411	The γ-ray irradiation method	384:411	The γ-ray irradiation method	384:411	The γ-ray irradiation method has advantages, such as relatively simple manipulation without need of any extra reagents for polymerization and cross-linking.
24507324	8	74	theme	content	1105:1111	arg1	concentrations					1054:1067	higher concentrations	1047:1067	higher concentrations of hyaluronidase solution and HA/CS content	1047:1111	Hydrogels with higher concentrations of hyaluronidase solution and HA/CS content had proportionally higher enzymatic degradation rates.
24507324	1	75	theme	hydrogels	123:131	arg1	irradiation					102:112	Gamma-ray irradiation	92:112	Gamma-ray irradiation of novel hydrogels	92:131	Gamma-ray irradiation of novel hydrogels was used to develop a biocompatible hydrogel system for skin tissue engineering.
24507324	13	76	theme	HA/CA/PVA	1669:1677	arg1	hydrogels					1679:1687	all HA/CA/PVA hydrogels	1665:1687	all HA/CA/PVA hydrogels	1665:1687	After 7 days, the HaCaT cells in all HA/CA/PVA hydrogels exhibited more than 80% viability compared to the control group HaCaT culture on a culture plate.
24507324	11	77	theme	hydrogels	1488:1496	arg1	extracts					1466:1473	the extracts	1462:1473	the extracts of HA/CS/PVA hydrogels	1462:1496	All HaCaT cell cultures exposed to the extracts of HA/CS/PVA hydrogels exhibited greater than 92% cell viability.
24507324	13	78	theme	group	1747:1751	arg1	culture					1759:1765	the control group HaCaT culture	1735:1765	the control group HaCaT culture on a culture plate	1735:1784	After 7 days, the HaCaT cells in all HA/CA/PVA hydrogels exhibited more than 80% viability compared to the control group HaCaT culture on a culture plate.
24507324	0	79	theme	irradiation	69:79	arg1	technique					81:89	gamma irradiation technique	63:89	gamma irradiation technique	63:89	Hyaluronic acid/chondroitin sulfate-based hydrogel prepared by gamma irradiation technique.
24507324	8	80	theme	enzymatic	1139:1147	arg1	rates					1161:1165	proportionally higher enzymatic degradation rates	1117:1165	proportionally higher enzymatic degradation rates	1117:1165	Hydrogels with higher concentrations of hyaluronidase solution and HA/CS content had proportionally higher enzymatic degradation rates.
24507324	12	81	theme	HaCaT	1545:1549	arg1	growth					1551:1556	The HaCaT growth	1541:1556	The HaCaT growth in HA/CS/PVA hydrogels	1541:1579	The HaCaT growth in HA/CS/PVA hydrogels gradually increased as a function of culture time.
24507324	5	82	with	hydrogels	621:629	arg1	compositions					644:655	various compositions	636:655	various compositions	636:655	The HA/CS/PVA hydrogels with various compositions were prepared by using γ-ray irradiation technique and their physicochemical properties were investigated to evaluate the feasibility of their use as artificial skin substitutes.
24507324	13	83	theme	HaCaT	1650:1654	arg1	cells					1656:1660	the HaCaT cells	1646:1660	the HaCaT cells in all HA/CA/PVA hydrogels	1646:1687	After 7 days, the HaCaT cells in all HA/CA/PVA hydrogels exhibited more than 80% viability compared to the control group HaCaT culture on a culture plate.
24507324	10	84	theme	hydrogels	1368:1376	arg1	extracts					1346:1353	the extracts	1342:1353	the extracts of HA/CS/PVA hydrogels	1342:1376	Exposure of human keratinocyte (HaCaT) culture to the extracts of HA/CS/PVA hydrogels did not significantly affect the cell viability.
24507324	7	85	theme	HA/CS/PVA	920:928	arg1	hydrogels					930:938	All HA/CS/PVA hydrogels	916:938	All HA/CS/PVA hydrogels	916:938	All HA/CS/PVA hydrogels exhibited more than 90% water content and reached an equilibrium swelling state within 24h.
24507324	4	86	theme	polymerizable	583:595	arg1	residues					597:604	polymerizable residues	583:604	polymerizable residues	583:604	We synthesized HA and CS derivatives with polymerizable residues.
24507324	2	87	theme	novel	220:224	arg1	hydrogels					226:234	These novel hydrogels	214:234	These novel hydrogels	214:234	These novel hydrogels are composed of natural polymers including hyaluronic acid (HA) and chondroitin sulfate (CS), and the synthetic polymer, poly(vinyl alcohol) (PVA).
24507324	10	88	theme	cell	1411:1414	arg1	viability					1416:1424	the cell viability	1407:1424	the cell viability	1407:1424	Exposure of human keratinocyte (HaCaT) culture to the extracts of HA/CS/PVA hydrogels did not significantly affect the cell viability.
24507324	3	89	theme	simple	448:453	arg1	manipulation					455:466	relatively simple manipulation	437:466	relatively simple manipulation without need of any extra reagents for polymerization and cross-linking	437:538	The γ-ray irradiation method has advantages, such as relatively simple manipulation without need of any extra reagents for polymerization and cross-linking.
24507324	11	90	theme	HaCaT	1431:1435	arg1	cultures					1442:1449	All HaCaT cell cultures	1427:1449	All HaCaT cell cultures exposed to the extracts of HA/CS/PVA hydrogels	1427:1496	All HaCaT cell cultures exposed to the extracts of HA/CS/PVA hydrogels exhibited greater than 92% cell viability.
24507324	9	91	theme	HA/CS/PVA	1200:1208	arg1	hydrogels					1210:1218	HA/CS/PVA hydrogels	1200:1218	HA/CS/PVA hydrogels	1200:1218	The drug release behaviors from HA/CS/PVA hydrogels were influenced by the composition of the hydrogel and drug properties.
24507324	3	92	theme	extra	488:492	arg1	reagents					494:501	any extra reagents	484:501	any extra reagents	484:501	The γ-ray irradiation method has advantages, such as relatively simple manipulation without need of any extra reagents for polymerization and cross-linking.
27987938	1	0	theme	composites	303:312	arg1	dynamics					214:221	the molecular dynamics	200:221	the molecular dynamics of dried Bacterial Cellulose/Carboxymethyl Cellulose-Graphene Oxide (BC/CMC-GO) composites	200:312	Broadband Dielectric Relaxation Spectroscopy was performed to study the molecular dynamics of dried Bacterial Cellulose/Carboxymethyl Cellulose-Graphene Oxide (BC/CMC-GO) composites as a function of the concentration of CMC in the culture media.
27987938	1	0	theme	composites	303:312	arg1	function					319:326	a function	317:326	a function of the concentration of CMC in the culture media	317:375	Broadband Dielectric Relaxation Spectroscopy was performed to study the molecular dynamics of dried Bacterial Cellulose/Carboxymethyl Cellulose-Graphene Oxide (BC/CMC-GO) composites as a function of the concentration of CMC in the culture media.
27987938	0	1	theme	cellulose	112:120	arg1	addition					122:129	carboxymethyl cellulose addition	98:129	carboxymethyl cellulose addition	98:129	Monitoring molecular dynamics of bacterial cellulose composites reinforced with graphene oxide by carboxymethyl cellulose addition.
27987938	5	2	from	change	871:876	arg1	BC					881:882	BC	881:882	BC	881:882	This structural change in BC, that involves a variation in inter- and intramolecular interactions (hydrogen-bonding interactions), affects steeply their molecular dynamics.
27987938	1	3	from	CMC	352:354	arg1	media					371:375	the culture media	359:375	the culture media	359:375	Broadband Dielectric Relaxation Spectroscopy was performed to study the molecular dynamics of dried Bacterial Cellulose/Carboxymethyl Cellulose-Graphene Oxide (BC/CMC-GO) composites as a function of the concentration of CMC in the culture media.
27987938	4	4	theme	ratio	848:852	arg1	decrease					826:833	a decrease	824:833	a decrease of the Iα/Iβ ratio	824:852	X-ray diffractometry measurements show that increasing the concentration of CMC promotes a decrease of the Iα/Iβ ratio.
27987938	0	5	theme	carboxymethyl	98:110	arg1	addition					122:129	carboxymethyl cellulose addition	98:129	carboxymethyl cellulose addition	98:129	Monitoring molecular dynamics of bacterial cellulose composites reinforced with graphene oxide by carboxymethyl cellulose addition.
27987938	4	6	theme	Iα/Iβ	842:846	arg1	ratio					848:852	the Iα/Iβ ratio	838:852	the Iα/Iβ ratio	838:852	X-ray diffractometry measurements show that increasing the concentration of CMC promotes a decrease of the Iα/Iβ ratio.
27987938	5	7	theme	intramolecular	925:938	arg1	interactions					940:951	inter- and intramolecular interactions	914:951	inter- and intramolecular interactions	914:951	This structural change in BC, that involves a variation in inter- and intramolecular interactions (hydrogen-bonding interactions), affects steeply their molecular dynamics.
27987938	2	8	theme	low	381:383	arg1	temperature					385:395	low temperature	381:395	low temperature	381:395	At low temperature the dielectric spectra are dominated by a dipolar process labelled as a β-relaxation, whereas electrode polarization and the contribution of dc-conductivity dominate the spectra at high temperatures and low frequency.
27987938	1	9	from	concentration	335:347	arg1	media					371:375	the culture media	359:375	the culture media	359:375	Broadband Dielectric Relaxation Spectroscopy was performed to study the molecular dynamics of dried Bacterial Cellulose/Carboxymethyl Cellulose-Graphene Oxide (BC/CMC-GO) composites as a function of the concentration of CMC in the culture media.
27987938	6	10	theme	strength	1119:1126	arg1	increase					1135:1142	an increase	1132:1142	an increase of the dc-conductivity	1132:1165	So, an increase of CMC concentration produces a significantly decrease of the β-relaxation strength and an increase of the dc-conductivity.
27987938	6	10	theme	strength	1119:1126	arg1	decrease					1090:1097	a significantly decrease	1074:1097	a significantly decrease of the β-relaxation strength	1074:1126	So, an increase of CMC concentration produces a significantly decrease of the β-relaxation strength and an increase of the dc-conductivity.
27987938	4	11	theme	X-ray	735:739	arg1	measurements					756:767	X-ray diffractometry measurements	735:767	X-ray diffractometry measurements	735:767	X-ray diffractometry measurements show that increasing the concentration of CMC promotes a decrease of the Iα/Iβ ratio.
27987938	2	12	theme	electrode	491:499	arg1	polarization					501:512	electrode polarization	491:512	electrode polarization	491:512	At low temperature the dielectric spectra are dominated by a dipolar process labelled as a β-relaxation, whereas electrode polarization and the contribution of dc-conductivity dominate the spectra at high temperatures and low frequency.
27987938	5	13	theme	structural	860:869	arg1	change					871:876	This structural change	855:876	This structural change	855:876	This structural change in BC, that involves a variation in inter- and intramolecular interactions (hydrogen-bonding interactions), affects steeply their molecular dynamics.
27987938	6	14	theme	β-relaxation	1106:1117	arg1	strength					1119:1126	the β-relaxation strength	1102:1126	the β-relaxation strength	1102:1126	So, an increase of CMC concentration produces a significantly decrease of the β-relaxation strength and an increase of the dc-conductivity.
27987938	1	15	theme	molecular	204:212	arg1	dynamics					214:221	the molecular dynamics	200:221	the molecular dynamics of dried Bacterial Cellulose/Carboxymethyl Cellulose-Graphene Oxide (BC/CMC-GO) composites	200:312	Broadband Dielectric Relaxation Spectroscopy was performed to study the molecular dynamics of dried Bacterial Cellulose/Carboxymethyl Cellulose-Graphene Oxide (BC/CMC-GO) composites as a function of the concentration of CMC in the culture media.
27987938	1	15	theme	molecular	204:212	arg1	function					319:326	a function	317:326	a function of the concentration of CMC in the culture media	317:375	Broadband Dielectric Relaxation Spectroscopy was performed to study the molecular dynamics of dried Bacterial Cellulose/Carboxymethyl Cellulose-Graphene Oxide (BC/CMC-GO) composites as a function of the concentration of CMC in the culture media.
27987938	0	16	theme	molecular	11:19	arg1	dynamics					21:28	Monitoring molecular dynamics	0:28	Monitoring molecular dynamics of bacterial cellulose composites	0:62	Monitoring molecular dynamics of bacterial cellulose composites reinforced with graphene oxide by carboxymethyl cellulose addition.
27987938	3	17	theme	CMC	619:621	arg1	concentration					623:635	The CMC concentration	615:635	The CMC concentration	615:635	The CMC concentration affects the morphological structure of cellulose and subsequently alters its physical properties.
27987938	6	18	theme	dc-conductivity	1151:1165	arg1	increase					1135:1142	an increase	1132:1142	an increase of the dc-conductivity	1132:1165	So, an increase of CMC concentration produces a significantly decrease of the β-relaxation strength and an increase of the dc-conductivity.
27987938	6	18	theme	dc-conductivity	1151:1165	arg1	decrease					1090:1097	a significantly decrease	1074:1097	a significantly decrease of the β-relaxation strength	1074:1126	So, an increase of CMC concentration produces a significantly decrease of the β-relaxation strength and an increase of the dc-conductivity.
27987938	0	19	theme	Monitoring	0:9	arg1	dynamics					21:28	Monitoring molecular dynamics	0:28	Monitoring molecular dynamics of bacterial cellulose composites	0:62	Monitoring molecular dynamics of bacterial cellulose composites reinforced with graphene oxide by carboxymethyl cellulose addition.
27987938	6	20	theme	concentration	1051:1063	arg1	increase					1035:1042	an increase	1032:1042	an increase of CMC concentration	1032:1063	So, an increase of CMC concentration produces a significantly decrease of the β-relaxation strength and an increase of the dc-conductivity.
27987938	4	21	theme	diffractometry	741:754	arg1	measurements					756:767	X-ray diffractometry measurements	735:767	X-ray diffractometry measurements	735:767	X-ray diffractometry measurements show that increasing the concentration of CMC promotes a decrease of the Iα/Iβ ratio.
27987938	6	22	theme	CMC	1047:1049	arg1	concentration					1051:1063	CMC concentration	1047:1063	CMC concentration	1047:1063	So, an increase of CMC concentration produces a significantly decrease of the β-relaxation strength and an increase of the dc-conductivity.
27987938	1	23	theme	dried	226:230	arg1	composites					303:312	dried Bacterial Cellulose/Carboxymethyl Cellulose-Graphene Oxide (BC/CMC-GO) composites	226:312	dried Bacterial Cellulose/Carboxymethyl Cellulose-Graphene Oxide (BC/CMC-GO) composites	226:312	Broadband Dielectric Relaxation Spectroscopy was performed to study the molecular dynamics of dried Bacterial Cellulose/Carboxymethyl Cellulose-Graphene Oxide (BC/CMC-GO) composites as a function of the concentration of CMC in the culture media.
27987938	1	24	theme	concentration	335:347	arg1	dynamics					214:221	the molecular dynamics	200:221	the molecular dynamics of dried Bacterial Cellulose/Carboxymethyl Cellulose-Graphene Oxide (BC/CMC-GO) composites	200:312	Broadband Dielectric Relaxation Spectroscopy was performed to study the molecular dynamics of dried Bacterial Cellulose/Carboxymethyl Cellulose-Graphene Oxide (BC/CMC-GO) composites as a function of the concentration of CMC in the culture media.
27987938	1	24	theme	concentration	335:347	arg1	function					319:326	a function	317:326	a function of the concentration of CMC in the culture media	317:375	Broadband Dielectric Relaxation Spectroscopy was performed to study the molecular dynamics of dried Bacterial Cellulose/Carboxymethyl Cellulose-Graphene Oxide (BC/CMC-GO) composites as a function of the concentration of CMC in the culture media.
27987938	2	25	theme	low	600:602	arg1	frequency					604:612	low frequency	600:612	low frequency	600:612	At low temperature the dielectric spectra are dominated by a dipolar process labelled as a β-relaxation, whereas electrode polarization and the contribution of dc-conductivity dominate the spectra at high temperatures and low frequency.
27987938	3	26	theme	physical	714:721	arg1	properties					723:732	its physical properties	710:732	its physical properties	710:732	The CMC concentration affects the morphological structure of cellulose and subsequently alters its physical properties.
27987938	1	27	theme	Bacterial	232:240	arg1	composites					303:312	dried Bacterial Cellulose/Carboxymethyl Cellulose-Graphene Oxide (BC/CMC-GO) composites	226:312	dried Bacterial Cellulose/Carboxymethyl Cellulose-Graphene Oxide (BC/CMC-GO) composites	226:312	Broadband Dielectric Relaxation Spectroscopy was performed to study the molecular dynamics of dried Bacterial Cellulose/Carboxymethyl Cellulose-Graphene Oxide (BC/CMC-GO) composites as a function of the concentration of CMC in the culture media.
27987938	0	28	theme	cellulose	43:51	arg1	composites					53:62	bacterial cellulose composites	33:62	bacterial cellulose composites	33:62	Monitoring molecular dynamics of bacterial cellulose composites reinforced with graphene oxide by carboxymethyl cellulose addition.
27987938	5	29	theme	inter-	914:919	arg1	interactions					940:951	inter- and intramolecular interactions	914:951	inter- and intramolecular interactions	914:951	This structural change in BC, that involves a variation in inter- and intramolecular interactions (hydrogen-bonding interactions), affects steeply their molecular dynamics.
27987938	3	30	theme	morphological	649:661	arg1	structure					663:671	the morphological structure	645:671	the morphological structure of cellulose	645:684	The CMC concentration affects the morphological structure of cellulose and subsequently alters its physical properties.
27987938	5	31	theme	hydrogen-bonding	954:969	arg1	interactions					971:982	hydrogen-bonding interactions	954:982	hydrogen-bonding interactions	954:982	This structural change in BC, that involves a variation in inter- and intramolecular interactions (hydrogen-bonding interactions), affects steeply their molecular dynamics.
27987938	1	32	theme	Cellulose/Carboxymethyl	242:264	arg1	composites					303:312	dried Bacterial Cellulose/Carboxymethyl Cellulose-Graphene Oxide (BC/CMC-GO) composites	226:312	dried Bacterial Cellulose/Carboxymethyl Cellulose-Graphene Oxide (BC/CMC-GO) composites	226:312	Broadband Dielectric Relaxation Spectroscopy was performed to study the molecular dynamics of dried Bacterial Cellulose/Carboxymethyl Cellulose-Graphene Oxide (BC/CMC-GO) composites as a function of the concentration of CMC in the culture media.
27987938	0	33	theme	bacterial	33:41	arg1	composites					53:62	bacterial cellulose composites	33:62	bacterial cellulose composites	33:62	Monitoring molecular dynamics of bacterial cellulose composites reinforced with graphene oxide by carboxymethyl cellulose addition.
27987938	1	34	theme	CMC	352:354	arg1	concentration					335:347	the concentration	331:347	the concentration of CMC in the culture media	331:375	Broadband Dielectric Relaxation Spectroscopy was performed to study the molecular dynamics of dried Bacterial Cellulose/Carboxymethyl Cellulose-Graphene Oxide (BC/CMC-GO) composites as a function of the concentration of CMC in the culture media.
27987938	1	35	theme	Cellulose-Graphene	266:283	arg1	composites					303:312	dried Bacterial Cellulose/Carboxymethyl Cellulose-Graphene Oxide (BC/CMC-GO) composites	226:312	dried Bacterial Cellulose/Carboxymethyl Cellulose-Graphene Oxide (BC/CMC-GO) composites	226:312	Broadband Dielectric Relaxation Spectroscopy was performed to study the molecular dynamics of dried Bacterial Cellulose/Carboxymethyl Cellulose-Graphene Oxide (BC/CMC-GO) composites as a function of the concentration of CMC in the culture media.
27987938	1	36	from	media	371:375	arg1	concentration					335:347	the concentration	331:347	the concentration of CMC in the culture media	331:375	Broadband Dielectric Relaxation Spectroscopy was performed to study the molecular dynamics of dried Bacterial Cellulose/Carboxymethyl Cellulose-Graphene Oxide (BC/CMC-GO) composites as a function of the concentration of CMC in the culture media.
27987938	2	37	theme	high	578:581	arg1	temperatures					583:594	high temperatures	578:594	high temperatures	578:594	At low temperature the dielectric spectra are dominated by a dipolar process labelled as a β-relaxation, whereas electrode polarization and the contribution of dc-conductivity dominate the spectra at high temperatures and low frequency.
27987938	1	38	theme	Broadband	132:140	arg1	Spectroscopy					164:175	Broadband Dielectric Relaxation Spectroscopy	132:175	Broadband Dielectric Relaxation Spectroscopy	132:175	Broadband Dielectric Relaxation Spectroscopy was performed to study the molecular dynamics of dried Bacterial Cellulose/Carboxymethyl Cellulose-Graphene Oxide (BC/CMC-GO) composites as a function of the concentration of CMC in the culture media.
27987938	0	39	theme	composites	53:62	arg1	dynamics					21:28	Monitoring molecular dynamics	0:28	Monitoring molecular dynamics of bacterial cellulose composites	0:62	Monitoring molecular dynamics of bacterial cellulose composites reinforced with graphene oxide by carboxymethyl cellulose addition.
27987938	5	40	from	variation	901:909	arg1	interactions					940:951	inter- and intramolecular interactions	914:951	inter- and intramolecular interactions	914:951	This structural change in BC, that involves a variation in inter- and intramolecular interactions (hydrogen-bonding interactions), affects steeply their molecular dynamics.
27987938	1	41	theme	Dielectric	142:151	arg1	Spectroscopy					164:175	Broadband Dielectric Relaxation Spectroscopy	132:175	Broadband Dielectric Relaxation Spectroscopy	132:175	Broadband Dielectric Relaxation Spectroscopy was performed to study the molecular dynamics of dried Bacterial Cellulose/Carboxymethyl Cellulose-Graphene Oxide (BC/CMC-GO) composites as a function of the concentration of CMC in the culture media.
27987938	1	42	theme	culture	363:369	arg1	media					371:375	the culture media	359:375	the culture media	359:375	Broadband Dielectric Relaxation Spectroscopy was performed to study the molecular dynamics of dried Bacterial Cellulose/Carboxymethyl Cellulose-Graphene Oxide (BC/CMC-GO) composites as a function of the concentration of CMC in the culture media.
27987938	2	43	dep	dominated	424:432	arg1	whereas					483:489	whereas	483:489	whereas	483:489	At low temperature the dielectric spectra are dominated by a dipolar process labelled as a β-relaxation, whereas electrode polarization and the contribution of dc-conductivity dominate the spectra at high temperatures and low frequency.
27987938	1	44	theme	Relaxation	153:162	arg1	Spectroscopy					164:175	Broadband Dielectric Relaxation Spectroscopy	132:175	Broadband Dielectric Relaxation Spectroscopy	132:175	Broadband Dielectric Relaxation Spectroscopy was performed to study the molecular dynamics of dried Bacterial Cellulose/Carboxymethyl Cellulose-Graphene Oxide (BC/CMC-GO) composites as a function of the concentration of CMC in the culture media.
27987938	1	45	theme	Oxide	285:289	arg1	composites					303:312	dried Bacterial Cellulose/Carboxymethyl Cellulose-Graphene Oxide (BC/CMC-GO) composites	226:312	dried Bacterial Cellulose/Carboxymethyl Cellulose-Graphene Oxide (BC/CMC-GO) composites	226:312	Broadband Dielectric Relaxation Spectroscopy was performed to study the molecular dynamics of dried Bacterial Cellulose/Carboxymethyl Cellulose-Graphene Oxide (BC/CMC-GO) composites as a function of the concentration of CMC in the culture media.
27987938	4	46	theme	CMC	811:813	arg1	concentration					794:806	the concentration	790:806	the concentration of CMC	790:813	X-ray diffractometry measurements show that increasing the concentration of CMC promotes a decrease of the Iα/Iβ ratio.
27987938	5	47	theme	molecular	1008:1016	arg1	dynamics					1018:1025	their molecular dynamics	1002:1025	their molecular dynamics	1002:1025	This structural change in BC, that involves a variation in inter- and intramolecular interactions (hydrogen-bonding interactions), affects steeply their molecular dynamics.
27987938	0	48	theme	graphene	80:87	arg1	oxide					89:93	graphene oxide	80:93	graphene oxide	80:93	Monitoring molecular dynamics of bacterial cellulose composites reinforced with graphene oxide by carboxymethyl cellulose addition.
27987938	2	49	theme	dipolar	439:445	arg1	process					447:453	a dipolar process	437:453	a dipolar process labelled as a β-relaxation	437:480	At low temperature the dielectric spectra are dominated by a dipolar process labelled as a β-relaxation, whereas electrode polarization and the contribution of dc-conductivity dominate the spectra at high temperatures and low frequency.
27987938	1	50	theme	BC/CMC-GO	292:300	arg1	composites					303:312	dried Bacterial Cellulose/Carboxymethyl Cellulose-Graphene Oxide (BC/CMC-GO) composites	226:312	dried Bacterial Cellulose/Carboxymethyl Cellulose-Graphene Oxide (BC/CMC-GO) composites	226:312	Broadband Dielectric Relaxation Spectroscopy was performed to study the molecular dynamics of dried Bacterial Cellulose/Carboxymethyl Cellulose-Graphene Oxide (BC/CMC-GO) composites as a function of the concentration of CMC in the culture media.
27987938	2	51	theme	dc-conductivity	538:552	arg1	polarization					501:512	electrode polarization	491:512	electrode polarization	491:512	At low temperature the dielectric spectra are dominated by a dipolar process labelled as a β-relaxation, whereas electrode polarization and the contribution of dc-conductivity dominate the spectra at high temperatures and low frequency.
27987938	2	51	theme	dc-conductivity	538:552	arg1	contribution					522:533	the contribution	518:533	the contribution of dc-conductivity	518:552	At low temperature the dielectric spectra are dominated by a dipolar process labelled as a β-relaxation, whereas electrode polarization and the contribution of dc-conductivity dominate the spectra at high temperatures and low frequency.
27987938	3	52	theme	cellulose	676:684	arg1	structure					663:671	the morphological structure	645:671	the morphological structure of cellulose	645:684	The CMC concentration affects the morphological structure of cellulose and subsequently alters its physical properties.
27987938	2	53	theme	dielectric	401:410	arg1	spectra					412:418	the dielectric spectra	397:418	the dielectric spectra	397:418	At low temperature the dielectric spectra are dominated by a dipolar process labelled as a β-relaxation, whereas electrode polarization and the contribution of dc-conductivity dominate the spectra at high temperatures and low frequency.
29156586	6	0	theme	previous	909:916	arg1	study					918:922	A previous study	907:922	A previous study	907:922	A previous study has shown that Dendroaspis angusticeps venom contains not only a cocktail of classical toxins, but also small glycosylated peptides.
29156586	7	1	theme	laser	1244:1248	arg1	desorption/ionization					1250:1270	Matrix-assisted laser desorption/ionization	1228:1270	Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS)	1228:1318	Following this work, a deep exploration of DA glycopeptidome by a dual nano liquid chromatography coupled to electrospray ionization mass spectrometry (nanoLC-ESI-MS) and Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) analyses was initiated.
29156586	4	2	theme	main	577:580	arg1	toxins					562:567	These toxins	556:567	These toxins	556:567	These toxins are the main target of venom-based studies due to their valuable activities obtained by selectively targeting membrane receptors, such as ion channels or G-protein coupled receptors.
29156586	4	2	theme	main	577:580	arg1	target					582:587	the main target	573:587	the main target of venom-based studies due to their valuable activities obtained by selectively targeting membrane receptors, such as ion channels or G-protein coupled receptors	573:749	These toxins are the main target of venom-based studies due to their valuable activities obtained by selectively targeting membrane receptors, such as ion channels or G-protein coupled receptors.
29156586	10	3	theme	hydroxylation	1647:1659	arg1	presence					1611:1618	the presence	1607:1618	the presence of an S-cysteinylation and hydroxylation of proline on four glycopeptides, never described to date in snake venoms,	1607:1734	Finally, the presence of an S-cysteinylation and hydroxylation of proline on four glycopeptides, never described to date in snake venoms, is also revealed by proteomics and affined by nuclear magnetic resonance (NMR) experiments.
29156586	7	4	theme	DA	1100:1101	arg1	glycopeptidome					1103:1116	DA glycopeptidome	1100:1116	DA glycopeptidome by a dual nano liquid chromatography coupled to electrospray ionization mass spectrometry (nanoLC-ESI-MS)	1100:1222	Following this work, a deep exploration of DA glycopeptidome by a dual nano liquid chromatography coupled to electrospray ionization mass spectrometry (nanoLC-ESI-MS) and Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) analyses was initiated.
29156586	3	5	theme	several	529:535	arg1	bridges					547:553	several disulfide bridges	529:553	several disulfide bridges	529:553	It mainly contains hundreds of large toxins from 6 to 9 kDa, each displaying several disulfide bridges.
29156586	1	6	theme	innovative	303:312	arg1	drugs					314:318	even innovative drugs	298:318	even innovative drugs	298:318	Animal venoms represent a valuable source of bioactive peptides that can be derived into useful pharmacological tools, or even innovative drugs.
29156586	10	7	theme	S-cysteinylation	1626:1641	arg1	presence					1611:1618	the presence	1607:1618	the presence of an S-cysteinylation and hydroxylation of proline on four glycopeptides, never described to date in snake venoms,	1607:1734	Finally, the presence of an S-cysteinylation and hydroxylation of proline on four glycopeptides, never described to date in snake venoms, is also revealed by proteomics and affined by nuclear magnetic resonance (NMR) experiments.
29156586	0	8	theme	In-Depth	0:7	arg1	Approach					27:34	In-Depth Glyco-Peptidomics Approach	0:34	In-Depth Glyco-Peptidomics Approach	0:34	In-Depth Glyco-Peptidomics Approach Reveals Unexpected Diversity of Glycosylated Peptides and Atypical Post-Translational Modifications in Dendroaspis angusticeps Snake Venom.
29156586	5	9	theme	animal	849:854	arg1	venoms					856:861	animal venoms	849:861	animal venoms	849:861	This study aims to demonstrate that the knowledge of venom composition is still limited and that animal venoms contain unexpected diversity and surprises.
29156586	9	10	theme	structural	1506:1515	arg1	similarities					1517:1528	structural similarities	1506:1528	structural similarities with natriuretic peptides already characterized in Elapidae venoms	1506:1595	Sequence alignments underline structural similarities with natriuretic peptides already characterized in Elapidae venoms.
29156586	10	11	theme	proline	1664:1670	arg1	hydroxylation					1647:1659	hydroxylation	1647:1659	hydroxylation	1647:1659	Finally, the presence of an S-cysteinylation and hydroxylation of proline on four glycopeptides, never described to date in snake venoms, is also revealed by proteomics and affined by nuclear magnetic resonance (NMR) experiments.
29156586	10	11	theme	proline	1664:1670	arg1	S-cysteinylation					1626:1641	S-cysteinylation	1626:1641	S-cysteinylation	1626:1641	Finally, the presence of an S-cysteinylation and hydroxylation of proline on four glycopeptides, never described to date in snake venoms, is also revealed by proteomics and affined by nuclear magnetic resonance (NMR) experiments.
29156586	0	12	theme	Snake	163:167	arg1	Venom					169:173	Dendroaspis angusticeps Snake Venom	139:173	Dendroaspis angusticeps Snake Venom	139:173	In-Depth Glyco-Peptidomics Approach Reveals Unexpected Diversity of Glycosylated Peptides and Atypical Post-Translational Modifications in Dendroaspis angusticeps Snake Venom.
29156586	6	13	theme	angusticeps	951:961	arg1	venom					963:967	Dendroaspis angusticeps venom	939:967	Dendroaspis angusticeps venom	939:967	A previous study has shown that Dendroaspis angusticeps venom contains not only a cocktail of classical toxins, but also small glycosylated peptides.
29156586	4	14	theme	valuable	625:632	arg1	activities					634:643	their valuable activities	619:643	their valuable activities obtained by selectively targeting membrane receptors, such as ion channels or G-protein coupled receptors	619:749	These toxins are the main target of venom-based studies due to their valuable activities obtained by selectively targeting membrane receptors, such as ion channels or G-protein coupled receptors.
29156586	3	15	contain	contains	462:469	arg2	hundreds					471:478	hundreds	471:478	hundreds of large toxins from 6 to 9 kDa	471:510	It mainly contains hundreds of large toxins from 6 to 9 kDa, each displaying several disulfide bridges.
29156586	3	15	contain	contains	462:469	arg2	each					513:516	each	513:516	each	513:516	It mainly contains hundreds of large toxins from 6 to 9 kDa, each displaying several disulfide bridges.
29156586	3	15	contain	contains	462:469	arg1	It					452:453	It	452:453	It	452:453	It mainly contains hundreds of large toxins from 6 to 9 kDa, each displaying several disulfide bridges.
29156586	7	16	dep	exploration	1085:1095	arg1	analyses					1320:1327	analyses	1320:1327	analyses	1320:1327	Following this work, a deep exploration of DA glycopeptidome by a dual nano liquid chromatography coupled to electrospray ionization mass spectrometry (nanoLC-ESI-MS) and Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) analyses was initiated.
29156586	9	17	theme	Elapidae	1581:1588	arg1	venoms					1590:1595	Elapidae venoms	1581:1595	Elapidae venoms	1581:1595	Sequence alignments underline structural similarities with natriuretic peptides already characterized in Elapidae venoms.
29156586	4	18	theme	membrane	679:686	arg1	receptors					688:696	membrane receptors	679:696	membrane receptors	679:696	These toxins are the main target of venom-based studies due to their valuable activities obtained by selectively targeting membrane receptors, such as ion channels or G-protein coupled receptors.
29156586	4	18	theme	membrane	679:686	arg1	receptors					741:749	G-protein coupled receptors	723:749	G-protein coupled receptors	723:749	These toxins are the main target of venom-based studies due to their valuable activities obtained by selectively targeting membrane receptors, such as ion channels or G-protein coupled receptors.
29156586	4	18	theme	membrane	679:686	arg1	channels					711:718	ion channels	707:718	ion channels	707:718	These toxins are the main target of venom-based studies due to their valuable activities obtained by selectively targeting membrane receptors, such as ion channels or G-protein coupled receptors.
29156586	7	19	theme	Matrix-assisted	1228:1242	arg1	desorption/ionization					1250:1270	Matrix-assisted laser desorption/ionization	1228:1270	Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS)	1228:1318	Following this work, a deep exploration of DA glycopeptidome by a dual nano liquid chromatography coupled to electrospray ionization mass spectrometry (nanoLC-ESI-MS) and Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) analyses was initiated.
29156586	6	20	theme	classical	1001:1009	arg1	toxins					1011:1016	classical toxins	1001:1016	classical toxins	1001:1016	A previous study has shown that Dendroaspis angusticeps venom contains not only a cocktail of classical toxins, but also small glycosylated peptides.
29156586	0	21	theme	Atypical	94:101	arg1	Modifications					122:134	Atypical Post-Translational Modifications	94:134	Atypical Post-Translational Modifications	94:134	In-Depth Glyco-Peptidomics Approach Reveals Unexpected Diversity of Glycosylated Peptides and Atypical Post-Translational Modifications in Dendroaspis angusticeps Snake Venom.
29156586	1	22	theme	valuable	202:209	arg1	source					211:216	a valuable source	200:216	a valuable source of bioactive peptides that can be derived into useful pharmacological tools, or even innovative drugs	200:318	Animal venoms represent a valuable source of bioactive peptides that can be derived into useful pharmacological tools, or even innovative drugs.
29156586	8	23	theme	unsuspected	1363:1373	arg1	diversity					1386:1394	unsuspected structural diversity	1363:1394	unsuspected structural diversity of compounds such as 221 glycopeptides, displaying different glycan structures	1363:1473	This study reveals unsuspected structural diversity of compounds such as 221 glycopeptides, displaying different glycan structures.
29156586	0	24	theme	Modifications	122:134	arg1	Diversity					55:63	Unexpected Diversity	44:63	Unexpected Diversity of Glycosylated Peptides and Atypical Post-Translational Modifications in Dendroaspis angusticeps Snake Venom	44:173	In-Depth Glyco-Peptidomics Approach Reveals Unexpected Diversity of Glycosylated Peptides and Atypical Post-Translational Modifications in Dendroaspis angusticeps Snake Venom.
29156586	1	25	theme	Animal	176:181	arg1	venoms					183:188	Animal venoms	176:188	Animal venoms	176:188	Animal venoms represent a valuable source of bioactive peptides that can be derived into useful pharmacological tools, or even innovative drugs.
29156586	0	26	theme	Dendroaspis	139:149	arg1	Venom					169:173	Dendroaspis angusticeps Snake Venom	139:173	Dendroaspis angusticeps Snake Venom	139:173	In-Depth Glyco-Peptidomics Approach Reveals Unexpected Diversity of Glycosylated Peptides and Atypical Post-Translational Modifications in Dendroaspis angusticeps Snake Venom.
29156586	10	27	gly	glycopeptides	1680:1692	arg2	glycopeptides					1680:1692	four glycopeptides	1675:1692	four glycopeptides	1675:1692	Finally, the presence of an S-cysteinylation and hydroxylation of proline on four glycopeptides, never described to date in snake venoms, is also revealed by proteomics and affined by nuclear magnetic resonance (NMR) experiments.
29156586	4	28	theme	G-protein	723:731	arg1	receptors					741:749	G-protein coupled receptors	723:749	G-protein coupled receptors	723:749	These toxins are the main target of venom-based studies due to their valuable activities obtained by selectively targeting membrane receptors, such as ion channels or G-protein coupled receptors.
29156586	2	29	theme	Green	389:393	arg1	Mamba					395:399	the Eastern Green Mamba	377:399	the Eastern Green Mamba	377:399	In this way, the venom of Dendroaspis angusticeps (DA), the Eastern Green Mamba, has been intensively studied during recent years.
29156586	2	29	theme	Green	389:393	arg1	angusticeps					359:369	Dendroaspis angusticeps	347:369	Dendroaspis angusticeps (DA)	347:374	In this way, the venom of Dendroaspis angusticeps (DA), the Eastern Green Mamba, has been intensively studied during recent years.
29156586	7	30	theme	electrospray	1166:1177	arg1	spectrometry					1195:1206	electrospray ionization mass spectrometry	1166:1206	electrospray ionization mass spectrometry (nanoLC-ESI-MS)	1166:1222	Following this work, a deep exploration of DA glycopeptidome by a dual nano liquid chromatography coupled to electrospray ionization mass spectrometry (nanoLC-ESI-MS) and Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) analyses was initiated.
29156586	7	30	theme	electrospray	1166:1177	arg1	nanoLC-ESI-MS					1209:1221	nanoLC-ESI-MS	1209:1221	nanoLC-ESI-MS	1209:1221	Following this work, a deep exploration of DA glycopeptidome by a dual nano liquid chromatography coupled to electrospray ionization mass spectrometry (nanoLC-ESI-MS) and Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) analyses was initiated.
29156586	2	31	theme	recent	438:443	arg1	years					445:449	recent years	438:449	recent years	438:449	In this way, the venom of Dendroaspis angusticeps (DA), the Eastern Green Mamba, has been intensively studied during recent years.
29156586	6	32	gly	glycosylated	1034:1045	arg1	peptides					1047:1054	small glycosylated peptides	1028:1054	small glycosylated peptides	1028:1054	A previous study has shown that Dendroaspis angusticeps venom contains not only a cocktail of classical toxins, but also small glycosylated peptides.
29156586	5	33	theme	composition	811:821	arg1	knowledge					792:800	the knowledge	788:800	the knowledge of venom composition	788:821	This study aims to demonstrate that the knowledge of venom composition is still limited and that animal venoms contain unexpected diversity and surprises.
29156586	10	34	from	presence	1611:1618	arg1	glycopeptides					1680:1692	four glycopeptides	1675:1692	four glycopeptides	1675:1692	Finally, the presence of an S-cysteinylation and hydroxylation of proline on four glycopeptides, never described to date in snake venoms, is also revealed by proteomics and affined by nuclear magnetic resonance (NMR) experiments.
29156586	6	35	theme	glycosylated	1034:1045	arg1	peptides					1047:1054	small glycosylated peptides	1028:1054	small glycosylated peptides	1028:1054	A previous study has shown that Dendroaspis angusticeps venom contains not only a cocktail of classical toxins, but also small glycosylated peptides.
29156586	9	36	with	similarities	1517:1528	arg1	peptides					1547:1554	natriuretic peptides	1535:1554	natriuretic peptides already characterized in Elapidae venoms	1535:1595	Sequence alignments underline structural similarities with natriuretic peptides already characterized in Elapidae venoms.
29156586	0	37	theme	Unexpected	44:53	arg1	Diversity					55:63	Unexpected Diversity	44:63	Unexpected Diversity of Glycosylated Peptides and Atypical Post-Translational Modifications in Dendroaspis angusticeps Snake Venom	44:173	In-Depth Glyco-Peptidomics Approach Reveals Unexpected Diversity of Glycosylated Peptides and Atypical Post-Translational Modifications in Dendroaspis angusticeps Snake Venom.
29156586	7	38	theme	liquid	1133:1138	arg1	chromatography					1140:1153	a dual nano liquid chromatography	1121:1153	a dual nano liquid chromatography coupled to electrospray ionization mass spectrometry (nanoLC-ESI-MS)	1121:1222	Following this work, a deep exploration of DA glycopeptidome by a dual nano liquid chromatography coupled to electrospray ionization mass spectrometry (nanoLC-ESI-MS) and Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) analyses was initiated.
29156586	0	39	theme	Peptides	81:88	arg1	Diversity					55:63	Unexpected Diversity	44:63	Unexpected Diversity of Glycosylated Peptides and Atypical Post-Translational Modifications in Dendroaspis angusticeps Snake Venom	44:173	In-Depth Glyco-Peptidomics Approach Reveals Unexpected Diversity of Glycosylated Peptides and Atypical Post-Translational Modifications in Dendroaspis angusticeps Snake Venom.
29156586	6	40	contain	contains	969:976	arg1	venom					963:967	Dendroaspis angusticeps venom	939:967	Dendroaspis angusticeps venom	939:967	A previous study has shown that Dendroaspis angusticeps venom contains not only a cocktail of classical toxins, but also small glycosylated peptides.
29156586	6	40	contain	contains	969:976	arg2	peptides					1047:1054	small glycosylated peptides	1028:1054	small glycosylated peptides	1028:1054	A previous study has shown that Dendroaspis angusticeps venom contains not only a cocktail of classical toxins, but also small glycosylated peptides.
29156586	6	40	contain	contains	969:976	arg2	cocktail					989:996	a cocktail	987:996	a cocktail of classical toxins	987:1016	A previous study has shown that Dendroaspis angusticeps venom contains not only a cocktail of classical toxins, but also small glycosylated peptides.
29156586	3	41	theme	toxins	489:494	arg1	each					513:516	each	513:516	each	513:516	It mainly contains hundreds of large toxins from 6 to 9 kDa, each displaying several disulfide bridges.
29156586	3	41	theme	toxins	489:494	arg1	hundreds					471:478	hundreds	471:478	hundreds of large toxins from 6 to 9 kDa	471:510	It mainly contains hundreds of large toxins from 6 to 9 kDa, each displaying several disulfide bridges.
29156586	7	42	theme	dual	1123:1126	arg1	chromatography					1140:1153	a dual nano liquid chromatography	1121:1153	a dual nano liquid chromatography coupled to electrospray ionization mass spectrometry (nanoLC-ESI-MS)	1121:1222	Following this work, a deep exploration of DA glycopeptidome by a dual nano liquid chromatography coupled to electrospray ionization mass spectrometry (nanoLC-ESI-MS) and Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) analyses was initiated.
29156586	8	43	theme	glycan	1457:1462	arg1	structures					1464:1473	different glycan structures	1447:1473	different glycan structures	1447:1473	This study reveals unsuspected structural diversity of compounds such as 221 glycopeptides, displaying different glycan structures.
29156586	3	44	from	hundreds	471:478	arg1	kDa					508:510	6 to 9 kDa	501:510	6 to 9 kDa	501:510	It mainly contains hundreds of large toxins from 6 to 9 kDa, each displaying several disulfide bridges.
29156586	2	45	theme	Dendroaspis	347:357	arg1	angusticeps					359:369	Dendroaspis angusticeps	347:369	Dendroaspis angusticeps (DA)	347:374	In this way, the venom of Dendroaspis angusticeps (DA), the Eastern Green Mamba, has been intensively studied during recent years.
29156586	2	45	theme	Dendroaspis	347:357	arg1	Mamba					395:399	the Eastern Green Mamba	377:399	the Eastern Green Mamba	377:399	In this way, the venom of Dendroaspis angusticeps (DA), the Eastern Green Mamba, has been intensively studied during recent years.
29156586	2	45	theme	Dendroaspis	347:357	arg1	DA					372:373	DA	372:373	DA	372:373	In this way, the venom of Dendroaspis angusticeps (DA), the Eastern Green Mamba, has been intensively studied during recent years.
29156586	7	46	theme	mass	1287:1290	arg1	MALDI-TOF-MS					1306:1317	MALDI-TOF-MS	1306:1317	MALDI-TOF-MS	1306:1317	Following this work, a deep exploration of DA glycopeptidome by a dual nano liquid chromatography coupled to electrospray ionization mass spectrometry (nanoLC-ESI-MS) and Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) analyses was initiated.
29156586	7	46	theme	mass	1287:1290	arg1	spectrometry					1292:1303	flight mass spectrometry	1280:1303	flight mass spectrometry (MALDI-TOF-MS)	1280:1318	Following this work, a deep exploration of DA glycopeptidome by a dual nano liquid chromatography coupled to electrospray ionization mass spectrometry (nanoLC-ESI-MS) and Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) analyses was initiated.
29156586	10	47	theme	resonance	1799:1807	arg1	experiments					1815:1825	nuclear magnetic resonance (NMR) experiments	1782:1825	nuclear magnetic resonance (NMR) experiments	1782:1825	Finally, the presence of an S-cysteinylation and hydroxylation of proline on four glycopeptides, never described to date in snake venoms, is also revealed by proteomics and affined by nuclear magnetic resonance (NMR) experiments.
29156586	1	48	theme	pharmacological	272:286	arg1	tools					288:292	useful pharmacological tools	265:292	useful pharmacological tools	265:292	Animal venoms represent a valuable source of bioactive peptides that can be derived into useful pharmacological tools, or even innovative drugs.
29156586	4	49	theme	venom-based	592:602	arg1	studies					604:610	venom-based studies	592:610	venom-based studies	592:610	These toxins are the main target of venom-based studies due to their valuable activities obtained by selectively targeting membrane receptors, such as ion channels or G-protein coupled receptors.
29156586	7	50	theme	glycopeptidome	1103:1116	arg1	time					1272:1275	Matrix-assisted laser desorption/ionization time	1228:1275	Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS)	1228:1318	Following this work, a deep exploration of DA glycopeptidome by a dual nano liquid chromatography coupled to electrospray ionization mass spectrometry (nanoLC-ESI-MS) and Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) analyses was initiated.
29156586	7	50	theme	glycopeptidome	1103:1116	arg1	exploration					1085:1095	a deep exploration	1078:1095	a deep exploration of DA glycopeptidome by a dual nano liquid chromatography coupled to electrospray ionization mass spectrometry (nanoLC-ESI-MS)	1078:1222	Following this work, a deep exploration of DA glycopeptidome by a dual nano liquid chromatography coupled to electrospray ionization mass spectrometry (nanoLC-ESI-MS) and Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) analyses was initiated.
29156586	10	51	theme	nuclear	1782:1788	arg1	NMR					1810:1812	NMR	1810:1812	NMR	1810:1812	Finally, the presence of an S-cysteinylation and hydroxylation of proline on four glycopeptides, never described to date in snake venoms, is also revealed by proteomics and affined by nuclear magnetic resonance (NMR) experiments.
29156586	10	51	theme	nuclear	1782:1788	arg1	resonance					1799:1807	nuclear magnetic resonance	1782:1807	nuclear magnetic resonance (NMR) experiments	1782:1825	Finally, the presence of an S-cysteinylation and hydroxylation of proline on four glycopeptides, never described to date in snake venoms, is also revealed by proteomics and affined by nuclear magnetic resonance (NMR) experiments.
29156586	10	52	from	glycopeptides	1680:1692	arg1	presence					1611:1618	the presence	1607:1618	the presence of an S-cysteinylation and hydroxylation of proline on four glycopeptides, never described to date in snake venoms,	1607:1734	Finally, the presence of an S-cysteinylation and hydroxylation of proline on four glycopeptides, never described to date in snake venoms, is also revealed by proteomics and affined by nuclear magnetic resonance (NMR) experiments.
29156586	7	53	theme	desorption/ionization	1250:1270	arg1	time					1272:1275	Matrix-assisted laser desorption/ionization time	1228:1275	Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS)	1228:1318	Following this work, a deep exploration of DA glycopeptidome by a dual nano liquid chromatography coupled to electrospray ionization mass spectrometry (nanoLC-ESI-MS) and Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) analyses was initiated.
29156586	1	54	theme	even	298:301	arg1	drugs					314:318	even innovative drugs	298:318	even innovative drugs	298:318	Animal venoms represent a valuable source of bioactive peptides that can be derived into useful pharmacological tools, or even innovative drugs.
29156586	1	55	theme	useful	265:270	arg1	tools					288:292	useful pharmacological tools	265:292	useful pharmacological tools	265:292	Animal venoms represent a valuable source of bioactive peptides that can be derived into useful pharmacological tools, or even innovative drugs.
29156586	7	56	theme	deep	1080:1083	arg1	exploration					1085:1095	a deep exploration	1078:1095	a deep exploration of DA glycopeptidome by a dual nano liquid chromatography coupled to electrospray ionization mass spectrometry (nanoLC-ESI-MS)	1078:1222	Following this work, a deep exploration of DA glycopeptidome by a dual nano liquid chromatography coupled to electrospray ionization mass spectrometry (nanoLC-ESI-MS) and Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) analyses was initiated.
29156586	3	57	from	kDa	508:510	arg1	toxins					489:494	large toxins	483:494	large toxins from 6 to 9 kDa	483:510	It mainly contains hundreds of large toxins from 6 to 9 kDa, each displaying several disulfide bridges.
29156586	3	57	from	kDa	508:510	arg1	each					513:516	each	513:516	each	513:516	It mainly contains hundreds of large toxins from 6 to 9 kDa, each displaying several disulfide bridges.
29156586	3	57	from	kDa	508:510	arg1	hundreds					471:478	hundreds	471:478	hundreds of large toxins from 6 to 9 kDa	471:510	It mainly contains hundreds of large toxins from 6 to 9 kDa, each displaying several disulfide bridges.
29156586	9	58	theme	Sequence	1476:1483	arg1	alignments					1485:1494	Sequence alignments	1476:1494	Sequence alignments	1476:1494	Sequence alignments underline structural similarities with natriuretic peptides already characterized in Elapidae venoms.
29156586	1	59	theme	bioactive	221:229	arg1	peptides					231:238	bioactive peptides	221:238	bioactive peptides	221:238	Animal venoms represent a valuable source of bioactive peptides that can be derived into useful pharmacological tools, or even innovative drugs.
29156586	3	60	theme	disulfide	537:545	arg1	bridges					547:553	several disulfide bridges	529:553	several disulfide bridges	529:553	It mainly contains hundreds of large toxins from 6 to 9 kDa, each displaying several disulfide bridges.
29156586	1	61	theme	peptides	231:238	arg1	source					211:216	a valuable source	200:216	a valuable source of bioactive peptides that can be derived into useful pharmacological tools, or even innovative drugs	200:318	Animal venoms represent a valuable source of bioactive peptides that can be derived into useful pharmacological tools, or even innovative drugs.
29156586	9	62	theme	natriuretic	1535:1545	arg1	peptides					1547:1554	natriuretic peptides	1535:1554	natriuretic peptides already characterized in Elapidae venoms	1535:1595	Sequence alignments underline structural similarities with natriuretic peptides already characterized in Elapidae venoms.
29156586	6	63	theme	Dendroaspis	939:949	arg1	venom					963:967	Dendroaspis angusticeps venom	939:967	Dendroaspis angusticeps venom	939:967	A previous study has shown that Dendroaspis angusticeps venom contains not only a cocktail of classical toxins, but also small glycosylated peptides.
29156586	4	64	theme	ion	707:709	arg1	channels					711:718	ion channels	707:718	ion channels	707:718	These toxins are the main target of venom-based studies due to their valuable activities obtained by selectively targeting membrane receptors, such as ion channels or G-protein coupled receptors.
29156586	5	65	theme	unexpected	871:880	arg1	diversity					882:890	unexpected diversity	871:890	unexpected diversity	871:890	This study aims to demonstrate that the knowledge of venom composition is still limited and that animal venoms contain unexpected diversity and surprises.
29156586	6	66	theme	toxins	1011:1016	arg1	cocktail					989:996	a cocktail	987:996	a cocktail of classical toxins	987:1016	A previous study has shown that Dendroaspis angusticeps venom contains not only a cocktail of classical toxins, but also small glycosylated peptides.
29156586	6	66	theme	toxins	1011:1016	arg1	peptides					1047:1054	small glycosylated peptides	1028:1054	small glycosylated peptides	1028:1054	A previous study has shown that Dendroaspis angusticeps venom contains not only a cocktail of classical toxins, but also small glycosylated peptides.
29156586	0	67	theme	Post-Translational	103:120	arg1	Modifications					122:134	Atypical Post-Translational Modifications	94:134	Atypical Post-Translational Modifications	94:134	In-Depth Glyco-Peptidomics Approach Reveals Unexpected Diversity of Glycosylated Peptides and Atypical Post-Translational Modifications in Dendroaspis angusticeps Snake Venom.
29156586	10	68	dep	S-cysteinylation	1626:1641	arg1	an					1623:1624	an	1623:1624	an	1623:1624	Finally, the presence of an S-cysteinylation and hydroxylation of proline on four glycopeptides, never described to date in snake venoms, is also revealed by proteomics and affined by nuclear magnetic resonance (NMR) experiments.
29156586	5	69	contain	contain	863:869	arg2	surprises					896:904	surprises	896:904	surprises	896:904	This study aims to demonstrate that the knowledge of venom composition is still limited and that animal venoms contain unexpected diversity and surprises.
29156586	5	69	contain	contain	863:869	arg1	venoms					856:861	animal venoms	849:861	animal venoms	849:861	This study aims to demonstrate that the knowledge of venom composition is still limited and that animal venoms contain unexpected diversity and surprises.
29156586	5	69	contain	contain	863:869	arg2	diversity					882:890	unexpected diversity	871:890	unexpected diversity	871:890	This study aims to demonstrate that the knowledge of venom composition is still limited and that animal venoms contain unexpected diversity and surprises.
29156586	0	70	theme	angusticeps	151:161	arg1	Venom					169:173	Dendroaspis angusticeps Snake Venom	139:173	Dendroaspis angusticeps Snake Venom	139:173	In-Depth Glyco-Peptidomics Approach Reveals Unexpected Diversity of Glycosylated Peptides and Atypical Post-Translational Modifications in Dendroaspis angusticeps Snake Venom.
29156586	0	71	from	Diversity	55:63	arg1	Venom					169:173	Dendroaspis angusticeps Snake Venom	139:173	Dendroaspis angusticeps Snake Venom	139:173	In-Depth Glyco-Peptidomics Approach Reveals Unexpected Diversity of Glycosylated Peptides and Atypical Post-Translational Modifications in Dendroaspis angusticeps Snake Venom.
29156586	8	72	theme	structural	1375:1384	arg1	diversity					1386:1394	unsuspected structural diversity	1363:1394	unsuspected structural diversity of compounds such as 221 glycopeptides, displaying different glycan structures	1363:1473	This study reveals unsuspected structural diversity of compounds such as 221 glycopeptides, displaying different glycan structures.
29156586	7	73	theme	ionization	1179:1188	arg1	spectrometry					1195:1206	electrospray ionization mass spectrometry	1166:1206	electrospray ionization mass spectrometry (nanoLC-ESI-MS)	1166:1222	Following this work, a deep exploration of DA glycopeptidome by a dual nano liquid chromatography coupled to electrospray ionization mass spectrometry (nanoLC-ESI-MS) and Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) analyses was initiated.
29156586	7	73	theme	ionization	1179:1188	arg1	nanoLC-ESI-MS					1209:1221	nanoLC-ESI-MS	1209:1221	nanoLC-ESI-MS	1209:1221	Following this work, a deep exploration of DA glycopeptidome by a dual nano liquid chromatography coupled to electrospray ionization mass spectrometry (nanoLC-ESI-MS) and Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) analyses was initiated.
29156586	0	74	theme	Glyco-Peptidomics	9:25	arg1	Approach					27:34	In-Depth Glyco-Peptidomics Approach	0:34	In-Depth Glyco-Peptidomics Approach	0:34	In-Depth Glyco-Peptidomics Approach Reveals Unexpected Diversity of Glycosylated Peptides and Atypical Post-Translational Modifications in Dendroaspis angusticeps Snake Venom.
29156586	2	75	theme	Eastern	381:387	arg1	Mamba					395:399	the Eastern Green Mamba	377:399	the Eastern Green Mamba	377:399	In this way, the venom of Dendroaspis angusticeps (DA), the Eastern Green Mamba, has been intensively studied during recent years.
29156586	2	75	theme	Eastern	381:387	arg1	angusticeps					359:369	Dendroaspis angusticeps	347:369	Dendroaspis angusticeps (DA)	347:374	In this way, the venom of Dendroaspis angusticeps (DA), the Eastern Green Mamba, has been intensively studied during recent years.
29156586	5	76	theme	venom	805:809	arg1	composition					811:821	venom composition	805:821	venom composition	805:821	This study aims to demonstrate that the knowledge of venom composition is still limited and that animal venoms contain unexpected diversity and surprises.
29156586	3	77	dep	9	506:506	arg1	to					503:504	to	503:504	to	503:504	It mainly contains hundreds of large toxins from 6 to 9 kDa, each displaying several disulfide bridges.
29156586	8	78	theme	compounds	1399:1407	arg1	diversity					1386:1394	unsuspected structural diversity	1363:1394	unsuspected structural diversity of compounds such as 221 glycopeptides, displaying different glycan structures	1363:1473	This study reveals unsuspected structural diversity of compounds such as 221 glycopeptides, displaying different glycan structures.
29156586	4	79	theme	coupled	733:739	arg1	receptors					741:749	G-protein coupled receptors	723:749	G-protein coupled receptors	723:749	These toxins are the main target of venom-based studies due to their valuable activities obtained by selectively targeting membrane receptors, such as ion channels or G-protein coupled receptors.
29156586	7	80	theme	nano	1128:1131	arg1	chromatography					1140:1153	a dual nano liquid chromatography	1121:1153	a dual nano liquid chromatography coupled to electrospray ionization mass spectrometry (nanoLC-ESI-MS)	1121:1222	Following this work, a deep exploration of DA glycopeptidome by a dual nano liquid chromatography coupled to electrospray ionization mass spectrometry (nanoLC-ESI-MS) and Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) analyses was initiated.
29156586	6	81	theme	small	1028:1032	arg1	peptides					1047:1054	small glycosylated peptides	1028:1054	small glycosylated peptides	1028:1054	A previous study has shown that Dendroaspis angusticeps venom contains not only a cocktail of classical toxins, but also small glycosylated peptides.
29156586	7	82	theme	mass	1190:1193	arg1	spectrometry					1195:1206	electrospray ionization mass spectrometry	1166:1206	electrospray ionization mass spectrometry (nanoLC-ESI-MS)	1166:1222	Following this work, a deep exploration of DA glycopeptidome by a dual nano liquid chromatography coupled to electrospray ionization mass spectrometry (nanoLC-ESI-MS) and Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) analyses was initiated.
29156586	7	82	theme	mass	1190:1193	arg1	nanoLC-ESI-MS					1209:1221	nanoLC-ESI-MS	1209:1221	nanoLC-ESI-MS	1209:1221	Following this work, a deep exploration of DA glycopeptidome by a dual nano liquid chromatography coupled to electrospray ionization mass spectrometry (nanoLC-ESI-MS) and Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) analyses was initiated.
29156586	0	83	theme	Glycosylated	68:79	arg1	Peptides					81:88	Glycosylated Peptides	68:88	Glycosylated Peptides	68:88	In-Depth Glyco-Peptidomics Approach Reveals Unexpected Diversity of Glycosylated Peptides and Atypical Post-Translational Modifications in Dendroaspis angusticeps Snake Venom.
29156586	7	84	theme	spectrometry	1292:1303	arg1	time					1272:1275	Matrix-assisted laser desorption/ionization time	1228:1275	Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS)	1228:1318	Following this work, a deep exploration of DA glycopeptidome by a dual nano liquid chromatography coupled to electrospray ionization mass spectrometry (nanoLC-ESI-MS) and Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) analyses was initiated.
29156586	7	84	theme	spectrometry	1292:1303	arg1	exploration					1085:1095	a deep exploration	1078:1095	a deep exploration of DA glycopeptidome by a dual nano liquid chromatography coupled to electrospray ionization mass spectrometry (nanoLC-ESI-MS)	1078:1222	Following this work, a deep exploration of DA glycopeptidome by a dual nano liquid chromatography coupled to electrospray ionization mass spectrometry (nanoLC-ESI-MS) and Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) analyses was initiated.
29156586	2	85	theme	angusticeps	359:369	arg1	venom					338:342	the venom	334:342	the venom of Dendroaspis angusticeps (DA), the Eastern Green Mamba,	334:400	In this way, the venom of Dendroaspis angusticeps (DA), the Eastern Green Mamba, has been intensively studied during recent years.
29156586	10	86	from	S-cysteinylation	1626:1641	arg1	glycopeptides					1680:1692	four glycopeptides	1675:1692	four glycopeptides	1675:1692	Finally, the presence of an S-cysteinylation and hydroxylation of proline on four glycopeptides, never described to date in snake venoms, is also revealed by proteomics and affined by nuclear magnetic resonance (NMR) experiments.
29156586	7	87	theme	flight	1280:1285	arg1	MALDI-TOF-MS					1306:1317	MALDI-TOF-MS	1306:1317	MALDI-TOF-MS	1306:1317	Following this work, a deep exploration of DA glycopeptidome by a dual nano liquid chromatography coupled to electrospray ionization mass spectrometry (nanoLC-ESI-MS) and Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) analyses was initiated.
29156586	7	87	theme	flight	1280:1285	arg1	spectrometry					1292:1303	flight mass spectrometry	1280:1303	flight mass spectrometry (MALDI-TOF-MS)	1280:1318	Following this work, a deep exploration of DA glycopeptidome by a dual nano liquid chromatography coupled to electrospray ionization mass spectrometry (nanoLC-ESI-MS) and Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) analyses was initiated.
29156586	8	88	theme	different	1447:1455	arg1	structures					1464:1473	different glycan structures	1447:1473	different glycan structures	1447:1473	This study reveals unsuspected structural diversity of compounds such as 221 glycopeptides, displaying different glycan structures.
29156586	3	89	theme	large	483:487	arg1	toxins					489:494	large toxins	483:494	large toxins from 6 to 9 kDa	483:510	It mainly contains hundreds of large toxins from 6 to 9 kDa, each displaying several disulfide bridges.
29156586	8	90	gly	glycopeptides	1421:1433	arg2	glycopeptides					1421:1433	221 glycopeptides	1417:1433	221 glycopeptides	1417:1433	This study reveals unsuspected structural diversity of compounds such as 221 glycopeptides, displaying different glycan structures.
29156586	10	91	from	hydroxylation	1647:1659	arg1	glycopeptides					1680:1692	four glycopeptides	1675:1692	four glycopeptides	1675:1692	Finally, the presence of an S-cysteinylation and hydroxylation of proline on four glycopeptides, never described to date in snake venoms, is also revealed by proteomics and affined by nuclear magnetic resonance (NMR) experiments.
29156586	10	92	theme	snake	1722:1726	arg1	venoms					1728:1733	snake venoms	1722:1733	snake venoms	1722:1733	Finally, the presence of an S-cysteinylation and hydroxylation of proline on four glycopeptides, never described to date in snake venoms, is also revealed by proteomics and affined by nuclear magnetic resonance (NMR) experiments.
29156586	4	93	theme	studies	604:610	arg1	toxins					562:567	These toxins	556:567	These toxins	556:567	These toxins are the main target of venom-based studies due to their valuable activities obtained by selectively targeting membrane receptors, such as ion channels or G-protein coupled receptors.
29156586	4	93	theme	studies	604:610	arg1	target					582:587	the main target	573:587	the main target of venom-based studies due to their valuable activities obtained by selectively targeting membrane receptors, such as ion channels or G-protein coupled receptors	573:749	These toxins are the main target of venom-based studies due to their valuable activities obtained by selectively targeting membrane receptors, such as ion channels or G-protein coupled receptors.
29156586	10	94	theme	magnetic	1790:1797	arg1	NMR					1810:1812	NMR	1810:1812	NMR	1810:1812	Finally, the presence of an S-cysteinylation and hydroxylation of proline on four glycopeptides, never described to date in snake venoms, is also revealed by proteomics and affined by nuclear magnetic resonance (NMR) experiments.
29156586	10	94	theme	magnetic	1790:1797	arg1	resonance					1799:1807	nuclear magnetic resonance	1782:1807	nuclear magnetic resonance (NMR) experiments	1782:1825	Finally, the presence of an S-cysteinylation and hydroxylation of proline on four glycopeptides, never described to date in snake venoms, is also revealed by proteomics and affined by nuclear magnetic resonance (NMR) experiments.
26925765	5	0	attach	presence	1270:1277	arg2	components					1287:1296	CCNC components	1282:1296	CCNC components	1282:1296	The double-membrane hydrogel containing the chemically modified cellulose nanocrystals (CCNC) in the inner membrane hydrogel can provide the sustained drug release ascribed to the "nano-obstruction effect" and "nanolocking effect" induced by the presence of CCNC components in the hydrogels.
26925765	5	0	attach	presence	1270:1277	arg1	hydrogels					1305:1313	the hydrogels	1301:1313	the hydrogels	1301:1313	The double-membrane hydrogel containing the chemically modified cellulose nanocrystals (CCNC) in the inner membrane hydrogel can provide the sustained drug release ascribed to the "nano-obstruction effect" and "nanolocking effect" induced by the presence of CCNC components in the hydrogels.
26925765	2	1	theme	internal	379:386	arg1	hydrogel					398:405	an internal composite hydrogel	376:405	an internal composite hydrogel	376:405	The architecture of the double-membrane hydrogel involves an external membrane composed of neat alginate, and an internal composite hydrogel consolidates by electrostatic interactions between cationic CNC and anionic alginate.
26925765	5	2	theme	nano-obstruction	1205:1220	arg1	"					1228:1228	the "nano-obstruction effect"	1200:1228	the "nano-obstruction effect"	1200:1228	The double-membrane hydrogel containing the chemically modified cellulose nanocrystals (CCNC) in the inner membrane hydrogel can provide the sustained drug release ascribed to the "nano-obstruction effect" and "nanolocking effect" induced by the presence of CCNC components in the hydrogels.
26925765	2	3	theme	hydrogel	306:313	arg1	architecture					270:281	The architecture	266:281	The architecture of the double-membrane hydrogel	266:313	The architecture of the double-membrane hydrogel involves an external membrane composed of neat alginate, and an internal composite hydrogel consolidates by electrostatic interactions between cationic CNC and anionic alginate.
26925765	1	4	theme	cationic	206:213	arg1	CNC					239:241	CNC	239:241	CNC	239:241	A biocompatible hydrogel with a double-membrane structure is developed from cationic cellulose nanocrystals (CNC) and anionic alginate.
26925765	1	4	theme	cationic	206:213	arg1	nanocrystals					225:236	cationic cellulose nanocrystals	206:236	cationic cellulose nanocrystals (CNC)	206:242	A biocompatible hydrogel with a double-membrane structure is developed from cationic cellulose nanocrystals (CNC) and anionic alginate.
26925765	6	5	theme	first	1528:1532	arg1	release					1540:1546	the first quick release	1524:1546	the first quick release of one drug	1524:1558	Derived from natural polysaccharides (cellulose and alginate), the novel double-membrane structure hydrogel material developed in this study is biocompatible and can realize the complexing drugs release with the first quick release of one drug and the successively slow release of another drug, which is expected to achieve the synergistic release effects or potentially provide the solution to drug resistance in biomedical application.
26925765	4	6	theme	drug	937:940	arg1	membranes					920:928	two membranes	916:928	two membranes (rapid drug release of the outer hydrogel, and prolonged drug release of the inner hydrogel)	916:1021	Two drugs are introduced into the different membranes of the hydrogel, which will ensure the complexing drugs codelivery and the varied drugs release behaviors from two membranes (rapid drug release of the outer hydrogel, and prolonged drug release of the inner hydrogel).
26925765	4	6	theme	drug	937:940	arg1	release					942:948	rapid drug release	931:948	rapid drug release of the outer hydrogel	931:970	Two drugs are introduced into the different membranes of the hydrogel, which will ensure the complexing drugs codelivery and the varied drugs release behaviors from two membranes (rapid drug release of the outer hydrogel, and prolonged drug release of the inner hydrogel).
26925765	5	7	from	presence	1270:1277	arg1	hydrogels					1305:1313	the hydrogels	1301:1313	the hydrogels	1301:1313	The double-membrane hydrogel containing the chemically modified cellulose nanocrystals (CCNC) in the inner membrane hydrogel can provide the sustained drug release ascribed to the "nano-obstruction effect" and "nanolocking effect" induced by the presence of CCNC components in the hydrogels.
26925765	6	8	theme	natural	1329:1335	arg1	alginate					1368:1375	alginate	1368:1375	alginate	1368:1375	Derived from natural polysaccharides (cellulose and alginate), the novel double-membrane structure hydrogel material developed in this study is biocompatible and can realize the complexing drugs release with the first quick release of one drug and the successively slow release of another drug, which is expected to achieve the synergistic release effects or potentially provide the solution to drug resistance in biomedical application.
26925765	6	8	theme	natural	1329:1335	arg1	cellulose					1354:1362	cellulose	1354:1362	cellulose	1354:1362	Derived from natural polysaccharides (cellulose and alginate), the novel double-membrane structure hydrogel material developed in this study is biocompatible and can realize the complexing drugs release with the first quick release of one drug and the successively slow release of another drug, which is expected to achieve the synergistic release effects or potentially provide the solution to drug resistance in biomedical application.
26925765	6	8	theme	natural	1329:1335	arg1	polysaccharides					1337:1351	natural polysaccharides	1329:1351	natural polysaccharides (cellulose and alginate)	1329:1376	Derived from natural polysaccharides (cellulose and alginate), the novel double-membrane structure hydrogel material developed in this study is biocompatible and can realize the complexing drugs release with the first quick release of one drug and the successively slow release of another drug, which is expected to achieve the synergistic release effects or potentially provide the solution to drug resistance in biomedical application.
26925765	5	9	theme	modified	1079:1086	arg1	CCNC					1112:1115	CCNC	1112:1115	CCNC	1112:1115	The double-membrane hydrogel containing the chemically modified cellulose nanocrystals (CCNC) in the inner membrane hydrogel can provide the sustained drug release ascribed to the "nano-obstruction effect" and "nanolocking effect" induced by the presence of CCNC components in the hydrogels.
26925765	5	9	theme	modified	1079:1086	arg1	nanocrystals					1098:1109	the chemically modified cellulose nanocrystals	1064:1109	the chemically modified cellulose nanocrystals (CCNC)	1064:1116	The double-membrane hydrogel containing the chemically modified cellulose nanocrystals (CCNC) in the inner membrane hydrogel can provide the sustained drug release ascribed to the "nano-obstruction effect" and "nanolocking effect" induced by the presence of CCNC components in the hydrogels.
26925765	3	10	theme	adsorption	550:559	arg1	duration					561:568	the adsorption duration	546:568	the adsorption duration of neat alginate	546:585	The thickness of the outer layer can be regulated by the adsorption duration of neat alginate, and the shape of the inner layer can directly determine the morphology and dimensions of the double-membrane hydrogel (microsphere, capsule, and filmlike shapes).
26925765	6	11	dep	polysaccharides	1337:1351	arg1	alginate					1368:1375	alginate	1368:1375	alginate	1368:1375	Derived from natural polysaccharides (cellulose and alginate), the novel double-membrane structure hydrogel material developed in this study is biocompatible and can realize the complexing drugs release with the first quick release of one drug and the successively slow release of another drug, which is expected to achieve the synergistic release effects or potentially provide the solution to drug resistance in biomedical application.
26925765	6	11	dep	polysaccharides	1337:1351	arg1	cellulose					1354:1362	cellulose	1354:1362	cellulose	1354:1362	Derived from natural polysaccharides (cellulose and alginate), the novel double-membrane structure hydrogel material developed in this study is biocompatible and can realize the complexing drugs release with the first quick release of one drug and the successively slow release of another drug, which is expected to achieve the synergistic release effects or potentially provide the solution to drug resistance in biomedical application.
26925765	6	11	dep	polysaccharides	1337:1351	arg1	polysaccharides					1337:1351	natural polysaccharides	1329:1351	natural polysaccharides (cellulose and alginate)	1329:1376	Derived from natural polysaccharides (cellulose and alginate), the novel double-membrane structure hydrogel material developed in this study is biocompatible and can realize the complexing drugs release with the first quick release of one drug and the successively slow release of another drug, which is expected to achieve the synergistic release effects or potentially provide the solution to drug resistance in biomedical application.
26925765	3	12	theme	layer	615:619	arg1	shape					596:600	the shape	592:600	the shape of the inner layer	592:619	The thickness of the outer layer can be regulated by the adsorption duration of neat alginate, and the shape of the inner layer can directly determine the morphology and dimensions of the double-membrane hydrogel (microsphere, capsule, and filmlike shapes).
26925765	4	13	theme	prolonged	977:985	arg1	membranes					920:928	two membranes	916:928	two membranes (rapid drug release of the outer hydrogel, and prolonged drug release of the inner hydrogel)	916:1021	Two drugs are introduced into the different membranes of the hydrogel, which will ensure the complexing drugs codelivery and the varied drugs release behaviors from two membranes (rapid drug release of the outer hydrogel, and prolonged drug release of the inner hydrogel).
26925765	4	13	theme	prolonged	977:985	arg1	release					992:998	prolonged drug release	977:998	prolonged drug release of the inner hydrogel	977:1020	Two drugs are introduced into the different membranes of the hydrogel, which will ensure the complexing drugs codelivery and the varied drugs release behaviors from two membranes (rapid drug release of the outer hydrogel, and prolonged drug release of the inner hydrogel).
26925765	6	14	theme	synergistic	1644:1654	arg1	effects					1664:1670	the synergistic release effects	1640:1670	the synergistic release effects	1640:1670	Derived from natural polysaccharides (cellulose and alginate), the novel double-membrane structure hydrogel material developed in this study is biocompatible and can realize the complexing drugs release with the first quick release of one drug and the successively slow release of another drug, which is expected to achieve the synergistic release effects or potentially provide the solution to drug resistance in biomedical application.
26925765	3	15	theme	outer	514:518	arg1	layer					520:524	the outer layer	510:524	the outer layer	510:524	The thickness of the outer layer can be regulated by the adsorption duration of neat alginate, and the shape of the inner layer can directly determine the morphology and dimensions of the double-membrane hydrogel (microsphere, capsule, and filmlike shapes).
26925765	5	16	theme	components	1287:1296	arg1	presence					1270:1277	the presence	1266:1277	the presence of CCNC components in the hydrogels	1266:1313	The double-membrane hydrogel containing the chemically modified cellulose nanocrystals (CCNC) in the inner membrane hydrogel can provide the sustained drug release ascribed to the "nano-obstruction effect" and "nanolocking effect" induced by the presence of CCNC components in the hydrogels.
26925765	4	17	dep	membranes	920:928	arg1	membranes					920:928	two membranes	916:928	two membranes (rapid drug release of the outer hydrogel, and prolonged drug release of the inner hydrogel)	916:1021	Two drugs are introduced into the different membranes of the hydrogel, which will ensure the complexing drugs codelivery and the varied drugs release behaviors from two membranes (rapid drug release of the outer hydrogel, and prolonged drug release of the inner hydrogel).
26925765	4	17	dep	membranes	920:928	arg1	release					942:948	rapid drug release	931:948	rapid drug release of the outer hydrogel	931:970	Two drugs are introduced into the different membranes of the hydrogel, which will ensure the complexing drugs codelivery and the varied drugs release behaviors from two membranes (rapid drug release of the outer hydrogel, and prolonged drug release of the inner hydrogel).
26925765	4	17	dep	membranes	920:928	arg1	release					992:998	prolonged drug release	977:998	prolonged drug release of the inner hydrogel	977:1020	Two drugs are introduced into the different membranes of the hydrogel, which will ensure the complexing drugs codelivery and the varied drugs release behaviors from two membranes (rapid drug release of the outer hydrogel, and prolonged drug release of the inner hydrogel).
26925765	5	18	theme	inner	1125:1129	arg1	hydrogel					1140:1147	the inner membrane hydrogel	1121:1147	the inner membrane hydrogel	1121:1147	The double-membrane hydrogel containing the chemically modified cellulose nanocrystals (CCNC) in the inner membrane hydrogel can provide the sustained drug release ascribed to the "nano-obstruction effect" and "nanolocking effect" induced by the presence of CCNC components in the hydrogels.
26925765	4	19	theme	outer	957:961	arg1	hydrogel					963:970	the outer hydrogel	953:970	the outer hydrogel	953:970	Two drugs are introduced into the different membranes of the hydrogel, which will ensure the complexing drugs codelivery and the varied drugs release behaviors from two membranes (rapid drug release of the outer hydrogel, and prolonged drug release of the inner hydrogel).
26925765	6	20	theme	biomedical	1730:1739	arg1	application					1741:1751	biomedical application	1730:1751	biomedical application	1730:1751	Derived from natural polysaccharides (cellulose and alginate), the novel double-membrane structure hydrogel material developed in this study is biocompatible and can realize the complexing drugs release with the first quick release of one drug and the successively slow release of another drug, which is expected to achieve the synergistic release effects or potentially provide the solution to drug resistance in biomedical application.
26925765	4	21	theme	different	785:793	arg1	membranes					795:803	the different membranes	781:803	the different membranes of the hydrogel, which will ensure the complexing drugs codelivery and the varied drugs release behaviors from two membranes (rapid drug release of the outer hydrogel, and prolonged drug release of the inner hydrogel)	781:1021	Two drugs are introduced into the different membranes of the hydrogel, which will ensure the complexing drugs codelivery and the varied drugs release behaviors from two membranes (rapid drug release of the outer hydrogel, and prolonged drug release of the inner hydrogel).
26925765	0	22	from	Nanocrystals	64:75	arg1	Hydrogels					30:38	Biocompatible Double-Membrane Hydrogels	0:38	Biocompatible Double-Membrane Hydrogels from Cationic Cellulose Nanocrystals and Anionic Alginate as Complexing Drugs	0:116	Biocompatible Double-Membrane Hydrogels from Cationic Cellulose Nanocrystals and Anionic Alginate as Complexing Drugs Codelivery.
26925765	0	23	theme	Alginate	89:96	arg1	Drugs					112:116	Anionic Alginate as Complexing Drugs	81:116	Anionic Alginate as Complexing Drugs	81:116	Biocompatible Double-Membrane Hydrogels from Cationic Cellulose Nanocrystals and Anionic Alginate as Complexing Drugs Codelivery.
26925765	2	24	theme	electrostatic	423:435	arg1	interactions					437:448	electrostatic interactions	423:448	electrostatic interactions between cationic CNC and anionic alginate	423:490	The architecture of the double-membrane hydrogel involves an external membrane composed of neat alginate, and an internal composite hydrogel consolidates by electrostatic interactions between cationic CNC and anionic alginate.
26925765	0	25	theme	Biocompatible	0:12	arg1	Hydrogels					30:38	Biocompatible Double-Membrane Hydrogels	0:38	Biocompatible Double-Membrane Hydrogels from Cationic Cellulose Nanocrystals and Anionic Alginate as Complexing Drugs	0:116	Biocompatible Double-Membrane Hydrogels from Cationic Cellulose Nanocrystals and Anionic Alginate as Complexing Drugs Codelivery.
26925765	1	26	with	hydrogel	146:153	arg1	structure					178:186	a double-membrane structure	160:186	a double-membrane structure	160:186	A biocompatible hydrogel with a double-membrane structure is developed from cationic cellulose nanocrystals (CNC) and anionic alginate.
26925765	6	27	from	solution	1699:1706	arg1	application					1741:1751	biomedical application	1730:1751	biomedical application	1730:1751	Derived from natural polysaccharides (cellulose and alginate), the novel double-membrane structure hydrogel material developed in this study is biocompatible and can realize the complexing drugs release with the first quick release of one drug and the successively slow release of another drug, which is expected to achieve the synergistic release effects or potentially provide the solution to drug resistance in biomedical application.
26925765	2	28	theme	anionic	475:481	arg1	alginate					483:490	anionic alginate	475:490	anionic alginate	475:490	The architecture of the double-membrane hydrogel involves an external membrane composed of neat alginate, and an internal composite hydrogel consolidates by electrostatic interactions between cationic CNC and anionic alginate.
26925765	4	29	dep	drugs	855:859	arg1	codelivery					861:870	codelivery	861:870	the complexing drugs codelivery	840:870	Two drugs are introduced into the different membranes of the hydrogel, which will ensure the complexing drugs codelivery and the varied drugs release behaviors from two membranes (rapid drug release of the outer hydrogel, and prolonged drug release of the inner hydrogel).
26925765	4	29	dep	drugs	855:859	arg1	behaviors					901:909	release behaviors	893:909	release behaviors	893:909	Two drugs are introduced into the different membranes of the hydrogel, which will ensure the complexing drugs codelivery and the varied drugs release behaviors from two membranes (rapid drug release of the outer hydrogel, and prolonged drug release of the inner hydrogel).
26925765	3	30	theme	double-membrane	681:695	arg1	hydrogel					697:704	the double-membrane hydrogel	677:704	the double-membrane hydrogel (microsphere, capsule, and filmlike shapes)	677:748	The thickness of the outer layer can be regulated by the adsorption duration of neat alginate, and the shape of the inner layer can directly determine the morphology and dimensions of the double-membrane hydrogel (microsphere, capsule, and filmlike shapes).
26925765	0	31	theme	Cationic	45:52	arg1	Nanocrystals					64:75	Cationic Cellulose Nanocrystals	45:75	Cationic Cellulose Nanocrystals	45:75	Biocompatible Double-Membrane Hydrogels from Cationic Cellulose Nanocrystals and Anionic Alginate as Complexing Drugs Codelivery.
26925765	6	32	theme	hydrogel	1415:1422	arg1	material					1424:1431	the novel double-membrane structure hydrogel material	1379:1431	the novel double-membrane structure hydrogel material developed in this study	1379:1455	Derived from natural polysaccharides (cellulose and alginate), the novel double-membrane structure hydrogel material developed in this study is biocompatible and can realize the complexing drugs release with the first quick release of one drug and the successively slow release of another drug, which is expected to achieve the synergistic release effects or potentially provide the solution to drug resistance in biomedical application.
26925765	6	32	theme	hydrogel	1415:1422	arg1	biocompatible					1460:1472	biocompatible	1460:1472	biocompatible	1460:1472	Derived from natural polysaccharides (cellulose and alginate), the novel double-membrane structure hydrogel material developed in this study is biocompatible and can realize the complexing drugs release with the first quick release of one drug and the successively slow release of another drug, which is expected to achieve the synergistic release effects or potentially provide the solution to drug resistance in biomedical application.
26925765	5	33	contain	containing	1053:1062	arg2	CCNC					1112:1115	CCNC	1112:1115	CCNC	1112:1115	The double-membrane hydrogel containing the chemically modified cellulose nanocrystals (CCNC) in the inner membrane hydrogel can provide the sustained drug release ascribed to the "nano-obstruction effect" and "nanolocking effect" induced by the presence of CCNC components in the hydrogels.
26925765	5	33	contain	containing	1053:1062	arg1	hydrogel					1044:1051	The double-membrane hydrogel	1024:1051	The double-membrane hydrogel containing the chemically modified cellulose nanocrystals (CCNC) in the inner membrane hydrogel	1024:1147	The double-membrane hydrogel containing the chemically modified cellulose nanocrystals (CCNC) in the inner membrane hydrogel can provide the sustained drug release ascribed to the "nano-obstruction effect" and "nanolocking effect" induced by the presence of CCNC components in the hydrogels.
26925765	5	33	contain	containing	1053:1062	arg2	nanocrystals					1098:1109	the chemically modified cellulose nanocrystals	1064:1109	the chemically modified cellulose nanocrystals (CCNC)	1064:1116	The double-membrane hydrogel containing the chemically modified cellulose nanocrystals (CCNC) in the inner membrane hydrogel can provide the sustained drug release ascribed to the "nano-obstruction effect" and "nanolocking effect" induced by the presence of CCNC components in the hydrogels.
26925765	0	34	theme	Anionic	81:87	arg1	Drugs					112:116	Anionic Alginate as Complexing Drugs	81:116	Anionic Alginate as Complexing Drugs	81:116	Biocompatible Double-Membrane Hydrogels from Cationic Cellulose Nanocrystals and Anionic Alginate as Complexing Drugs Codelivery.
26925765	4	35	theme	complexing	844:853	arg1	drugs					855:859	the complexing drugs	840:859	the complexing drugs codelivery	840:870	Two drugs are introduced into the different membranes of the hydrogel, which will ensure the complexing drugs codelivery and the varied drugs release behaviors from two membranes (rapid drug release of the outer hydrogel, and prolonged drug release of the inner hydrogel).
26925765	6	36	theme	double-membrane	1389:1403	arg1	material					1424:1431	the novel double-membrane structure hydrogel material	1379:1431	the novel double-membrane structure hydrogel material developed in this study	1379:1455	Derived from natural polysaccharides (cellulose and alginate), the novel double-membrane structure hydrogel material developed in this study is biocompatible and can realize the complexing drugs release with the first quick release of one drug and the successively slow release of another drug, which is expected to achieve the synergistic release effects or potentially provide the solution to drug resistance in biomedical application.
26925765	6	36	theme	double-membrane	1389:1403	arg1	biocompatible					1460:1472	biocompatible	1460:1472	biocompatible	1460:1472	Derived from natural polysaccharides (cellulose and alginate), the novel double-membrane structure hydrogel material developed in this study is biocompatible and can realize the complexing drugs release with the first quick release of one drug and the successively slow release of another drug, which is expected to achieve the synergistic release effects or potentially provide the solution to drug resistance in biomedical application.
26925765	5	37	theme	sustained	1165:1173	arg1	release					1180:1186	the sustained drug release	1161:1186	the sustained drug release ascribed to the "nano-obstruction effect" and "nanolocking effect" induced by the presence of CCNC components in the hydrogels	1161:1313	The double-membrane hydrogel containing the chemically modified cellulose nanocrystals (CCNC) in the inner membrane hydrogel can provide the sustained drug release ascribed to the "nano-obstruction effect" and "nanolocking effect" induced by the presence of CCNC components in the hydrogels.
26925765	5	38	theme	double-membrane	1028:1042	arg1	hydrogel					1044:1051	The double-membrane hydrogel	1024:1051	The double-membrane hydrogel containing the chemically modified cellulose nanocrystals (CCNC) in the inner membrane hydrogel	1024:1147	The double-membrane hydrogel containing the chemically modified cellulose nanocrystals (CCNC) in the inner membrane hydrogel can provide the sustained drug release ascribed to the "nano-obstruction effect" and "nanolocking effect" induced by the presence of CCNC components in the hydrogels.
26925765	4	39	theme	hydrogel	812:819	arg1	membranes					795:803	the different membranes	781:803	the different membranes of the hydrogel, which will ensure the complexing drugs codelivery and the varied drugs release behaviors from two membranes (rapid drug release of the outer hydrogel, and prolonged drug release of the inner hydrogel)	781:1021	Two drugs are introduced into the different membranes of the hydrogel, which will ensure the complexing drugs codelivery and the varied drugs release behaviors from two membranes (rapid drug release of the outer hydrogel, and prolonged drug release of the inner hydrogel).
26925765	6	40	theme	complexing	1494:1503	arg1	drugs					1505:1509	the complexing drugs	1490:1509	the complexing drugs release	1490:1517	Derived from natural polysaccharides (cellulose and alginate), the novel double-membrane structure hydrogel material developed in this study is biocompatible and can realize the complexing drugs release with the first quick release of one drug and the successively slow release of another drug, which is expected to achieve the synergistic release effects or potentially provide the solution to drug resistance in biomedical application.
26925765	1	41	theme	anionic	248:254	arg1	alginate					256:263	anionic alginate	248:263	anionic alginate	248:263	A biocompatible hydrogel with a double-membrane structure is developed from cationic cellulose nanocrystals (CNC) and anionic alginate.
26925765	3	42	theme	alginate	578:585	arg1	duration					561:568	the adsorption duration	546:568	the adsorption duration of neat alginate	546:585	The thickness of the outer layer can be regulated by the adsorption duration of neat alginate, and the shape of the inner layer can directly determine the morphology and dimensions of the double-membrane hydrogel (microsphere, capsule, and filmlike shapes).
26925765	5	43	theme	nanolocking	1235:1245	arg1	"					1253:1253	"nanolocking effect"	1234:1253	"nanolocking effect"	1234:1253	The double-membrane hydrogel containing the chemically modified cellulose nanocrystals (CCNC) in the inner membrane hydrogel can provide the sustained drug release ascribed to the "nano-obstruction effect" and "nanolocking effect" induced by the presence of CCNC components in the hydrogels.
26925765	4	44	theme	hydrogel	1013:1020	arg1	membranes					920:928	two membranes	916:928	two membranes (rapid drug release of the outer hydrogel, and prolonged drug release of the inner hydrogel)	916:1021	Two drugs are introduced into the different membranes of the hydrogel, which will ensure the complexing drugs codelivery and the varied drugs release behaviors from two membranes (rapid drug release of the outer hydrogel, and prolonged drug release of the inner hydrogel).
26925765	4	44	theme	hydrogel	1013:1020	arg1	release					942:948	rapid drug release	931:948	rapid drug release of the outer hydrogel	931:970	Two drugs are introduced into the different membranes of the hydrogel, which will ensure the complexing drugs codelivery and the varied drugs release behaviors from two membranes (rapid drug release of the outer hydrogel, and prolonged drug release of the inner hydrogel).
26925765	4	44	theme	hydrogel	1013:1020	arg1	release					992:998	prolonged drug release	977:998	prolonged drug release of the inner hydrogel	977:1020	Two drugs are introduced into the different membranes of the hydrogel, which will ensure the complexing drugs codelivery and the varied drugs release behaviors from two membranes (rapid drug release of the outer hydrogel, and prolonged drug release of the inner hydrogel).
26925765	3	45	theme	filmlike	733:740	arg1	shapes					742:747	filmlike shapes	733:747	filmlike shapes	733:747	The thickness of the outer layer can be regulated by the adsorption duration of neat alginate, and the shape of the inner layer can directly determine the morphology and dimensions of the double-membrane hydrogel (microsphere, capsule, and filmlike shapes).
26925765	2	46	theme	composite	388:396	arg1	hydrogel					398:405	an internal composite hydrogel	376:405	an internal composite hydrogel	376:405	The architecture of the double-membrane hydrogel involves an external membrane composed of neat alginate, and an internal composite hydrogel consolidates by electrostatic interactions between cationic CNC and anionic alginate.
26925765	3	47	dep	hydrogel	697:704	arg1	capsule					720:726	capsule	720:726	capsule	720:726	The thickness of the outer layer can be regulated by the adsorption duration of neat alginate, and the shape of the inner layer can directly determine the morphology and dimensions of the double-membrane hydrogel (microsphere, capsule, and filmlike shapes).
26925765	3	47	dep	hydrogel	697:704	arg1	microsphere					707:717	microsphere	707:717	microsphere	707:717	The thickness of the outer layer can be regulated by the adsorption duration of neat alginate, and the shape of the inner layer can directly determine the morphology and dimensions of the double-membrane hydrogel (microsphere, capsule, and filmlike shapes).
26925765	3	47	dep	hydrogel	697:704	arg1	shapes					742:747	filmlike shapes	733:747	filmlike shapes	733:747	The thickness of the outer layer can be regulated by the adsorption duration of neat alginate, and the shape of the inner layer can directly determine the morphology and dimensions of the double-membrane hydrogel (microsphere, capsule, and filmlike shapes).
26925765	4	48	theme	varied	880:885	arg1	drugs					887:891	the varied drugs	876:891	the varied drugs	876:891	Two drugs are introduced into the different membranes of the hydrogel, which will ensure the complexing drugs codelivery and the varied drugs release behaviors from two membranes (rapid drug release of the outer hydrogel, and prolonged drug release of the inner hydrogel).
26925765	2	49	theme	external	327:334	arg1	membrane					336:343	an external membrane	324:343	an external membrane composed of neat alginate	324:369	The architecture of the double-membrane hydrogel involves an external membrane composed of neat alginate, and an internal composite hydrogel consolidates by electrostatic interactions between cationic CNC and anionic alginate.
26925765	5	50	theme	effect	1222:1227	arg1	"					1228:1228	the "nano-obstruction effect"	1200:1228	the "nano-obstruction effect"	1200:1228	The double-membrane hydrogel containing the chemically modified cellulose nanocrystals (CCNC) in the inner membrane hydrogel can provide the sustained drug release ascribed to the "nano-obstruction effect" and "nanolocking effect" induced by the presence of CCNC components in the hydrogels.
26925765	4	51	theme	release	893:899	arg1	behaviors					901:909	release behaviors	893:909	release behaviors	893:909	Two drugs are introduced into the different membranes of the hydrogel, which will ensure the complexing drugs codelivery and the varied drugs release behaviors from two membranes (rapid drug release of the outer hydrogel, and prolonged drug release of the inner hydrogel).
26925765	3	52	theme	neat	573:576	arg1	alginate					578:585	neat alginate	573:585	neat alginate	573:585	The thickness of the outer layer can be regulated by the adsorption duration of neat alginate, and the shape of the inner layer can directly determine the morphology and dimensions of the double-membrane hydrogel (microsphere, capsule, and filmlike shapes).
26925765	2	53	theme	neat	357:360	arg1	alginate					362:369	neat alginate	357:369	neat alginate	357:369	The architecture of the double-membrane hydrogel involves an external membrane composed of neat alginate, and an internal composite hydrogel consolidates by electrostatic interactions between cationic CNC and anionic alginate.
26925765	6	54	theme	quick	1534:1538	arg1	release					1540:1546	the first quick release	1524:1546	the first quick release of one drug	1524:1558	Derived from natural polysaccharides (cellulose and alginate), the novel double-membrane structure hydrogel material developed in this study is biocompatible and can realize the complexing drugs release with the first quick release of one drug and the successively slow release of another drug, which is expected to achieve the synergistic release effects or potentially provide the solution to drug resistance in biomedical application.
26925765	1	55	theme	cellulose	215:223	arg1	CNC					239:241	CNC	239:241	CNC	239:241	A biocompatible hydrogel with a double-membrane structure is developed from cationic cellulose nanocrystals (CNC) and anionic alginate.
26925765	1	55	theme	cellulose	215:223	arg1	nanocrystals					225:236	cationic cellulose nanocrystals	206:236	cationic cellulose nanocrystals (CNC)	206:242	A biocompatible hydrogel with a double-membrane structure is developed from cationic cellulose nanocrystals (CNC) and anionic alginate.
26925765	4	56	theme	rapid	931:935	arg1	membranes					920:928	two membranes	916:928	two membranes (rapid drug release of the outer hydrogel, and prolonged drug release of the inner hydrogel)	916:1021	Two drugs are introduced into the different membranes of the hydrogel, which will ensure the complexing drugs codelivery and the varied drugs release behaviors from two membranes (rapid drug release of the outer hydrogel, and prolonged drug release of the inner hydrogel).
26925765	4	56	theme	rapid	931:935	arg1	release					942:948	rapid drug release	931:948	rapid drug release of the outer hydrogel	931:970	Two drugs are introduced into the different membranes of the hydrogel, which will ensure the complexing drugs codelivery and the varied drugs release behaviors from two membranes (rapid drug release of the outer hydrogel, and prolonged drug release of the inner hydrogel).
26925765	5	57	theme	cellulose	1088:1096	arg1	CCNC					1112:1115	CCNC	1112:1115	CCNC	1112:1115	The double-membrane hydrogel containing the chemically modified cellulose nanocrystals (CCNC) in the inner membrane hydrogel can provide the sustained drug release ascribed to the "nano-obstruction effect" and "nanolocking effect" induced by the presence of CCNC components in the hydrogels.
26925765	5	57	theme	cellulose	1088:1096	arg1	nanocrystals					1098:1109	the chemically modified cellulose nanocrystals	1064:1109	the chemically modified cellulose nanocrystals (CCNC)	1064:1116	The double-membrane hydrogel containing the chemically modified cellulose nanocrystals (CCNC) in the inner membrane hydrogel can provide the sustained drug release ascribed to the "nano-obstruction effect" and "nanolocking effect" induced by the presence of CCNC components in the hydrogels.
26925765	3	58	dep	morphology	648:657	arg1	the					644:646	the	644:646	the	644:646	The thickness of the outer layer can be regulated by the adsorption duration of neat alginate, and the shape of the inner layer can directly determine the morphology and dimensions of the double-membrane hydrogel (microsphere, capsule, and filmlike shapes).
26925765	3	59	theme	inner	609:613	arg1	layer					615:619	the inner layer	605:619	the inner layer	605:619	The thickness of the outer layer can be regulated by the adsorption duration of neat alginate, and the shape of the inner layer can directly determine the morphology and dimensions of the double-membrane hydrogel (microsphere, capsule, and filmlike shapes).
26925765	1	60	theme	biocompatible	132:144	arg1	hydrogel					146:153	A biocompatible hydrogel	130:153	A biocompatible hydrogel with a double-membrane structure	130:186	A biocompatible hydrogel with a double-membrane structure is developed from cationic cellulose nanocrystals (CNC) and anionic alginate.
26925765	2	61	theme	double-membrane	290:304	arg1	hydrogel					306:313	the double-membrane hydrogel	286:313	the double-membrane hydrogel	286:313	The architecture of the double-membrane hydrogel involves an external membrane composed of neat alginate, and an internal composite hydrogel consolidates by electrostatic interactions between cationic CNC and anionic alginate.
26925765	6	62	theme	slow	1581:1584	arg1	release					1586:1592	the successively slow release	1564:1592	the successively slow release of another drug, which is expected to achieve the synergistic release effects or potentially provide the solution to drug resistance in biomedical application	1564:1751	Derived from natural polysaccharides (cellulose and alginate), the novel double-membrane structure hydrogel material developed in this study is biocompatible and can realize the complexing drugs release with the first quick release of one drug and the successively slow release of another drug, which is expected to achieve the synergistic release effects or potentially provide the solution to drug resistance in biomedical application.
26925765	6	63	theme	release	1656:1662	arg1	effects					1664:1670	the synergistic release effects	1640:1670	the synergistic release effects	1640:1670	Derived from natural polysaccharides (cellulose and alginate), the novel double-membrane structure hydrogel material developed in this study is biocompatible and can realize the complexing drugs release with the first quick release of one drug and the successively slow release of another drug, which is expected to achieve the synergistic release effects or potentially provide the solution to drug resistance in biomedical application.
26925765	5	64	theme	CCNC	1282:1285	arg1	components					1287:1296	CCNC components	1282:1296	CCNC components	1282:1296	The double-membrane hydrogel containing the chemically modified cellulose nanocrystals (CCNC) in the inner membrane hydrogel can provide the sustained drug release ascribed to the "nano-obstruction effect" and "nanolocking effect" induced by the presence of CCNC components in the hydrogels.
26925765	6	65	theme	novel	1383:1387	arg1	material					1424:1431	the novel double-membrane structure hydrogel material	1379:1431	the novel double-membrane structure hydrogel material developed in this study	1379:1455	Derived from natural polysaccharides (cellulose and alginate), the novel double-membrane structure hydrogel material developed in this study is biocompatible and can realize the complexing drugs release with the first quick release of one drug and the successively slow release of another drug, which is expected to achieve the synergistic release effects or potentially provide the solution to drug resistance in biomedical application.
26925765	6	65	theme	novel	1383:1387	arg1	biocompatible					1460:1472	biocompatible	1460:1472	biocompatible	1460:1472	Derived from natural polysaccharides (cellulose and alginate), the novel double-membrane structure hydrogel material developed in this study is biocompatible and can realize the complexing drugs release with the first quick release of one drug and the successively slow release of another drug, which is expected to achieve the synergistic release effects or potentially provide the solution to drug resistance in biomedical application.
26925765	1	66	theme	double-membrane	162:176	arg1	structure					178:186	a double-membrane structure	160:186	a double-membrane structure	160:186	A biocompatible hydrogel with a double-membrane structure is developed from cationic cellulose nanocrystals (CNC) and anionic alginate.
26925765	0	67	theme	Complexing	101:110	arg1	Drugs					112:116	Anionic Alginate as Complexing Drugs	81:116	Anionic Alginate as Complexing Drugs	81:116	Biocompatible Double-Membrane Hydrogels from Cationic Cellulose Nanocrystals and Anionic Alginate as Complexing Drugs Codelivery.
26925765	5	68	theme	membrane	1131:1138	arg1	hydrogel					1140:1147	the inner membrane hydrogel	1121:1147	the inner membrane hydrogel	1121:1147	The double-membrane hydrogel containing the chemically modified cellulose nanocrystals (CCNC) in the inner membrane hydrogel can provide the sustained drug release ascribed to the "nano-obstruction effect" and "nanolocking effect" induced by the presence of CCNC components in the hydrogels.
26925765	6	69	theme	drug	1555:1558	arg1	release					1540:1546	the first quick release	1524:1546	the first quick release of one drug	1524:1558	Derived from natural polysaccharides (cellulose and alginate), the novel double-membrane structure hydrogel material developed in this study is biocompatible and can realize the complexing drugs release with the first quick release of one drug and the successively slow release of another drug, which is expected to achieve the synergistic release effects or potentially provide the solution to drug resistance in biomedical application.
26925765	6	69	theme	drug	1555:1558	arg1	release					1586:1592	the successively slow release	1564:1592	the successively slow release of another drug, which is expected to achieve the synergistic release effects or potentially provide the solution to drug resistance in biomedical application	1564:1751	Derived from natural polysaccharides (cellulose and alginate), the novel double-membrane structure hydrogel material developed in this study is biocompatible and can realize the complexing drugs release with the first quick release of one drug and the successively slow release of another drug, which is expected to achieve the synergistic release effects or potentially provide the solution to drug resistance in biomedical application.
26925765	4	70	theme	drug	987:990	arg1	membranes					920:928	two membranes	916:928	two membranes (rapid drug release of the outer hydrogel, and prolonged drug release of the inner hydrogel)	916:1021	Two drugs are introduced into the different membranes of the hydrogel, which will ensure the complexing drugs codelivery and the varied drugs release behaviors from two membranes (rapid drug release of the outer hydrogel, and prolonged drug release of the inner hydrogel).
26925765	4	70	theme	drug	987:990	arg1	release					992:998	prolonged drug release	977:998	prolonged drug release of the inner hydrogel	977:1020	Two drugs are introduced into the different membranes of the hydrogel, which will ensure the complexing drugs codelivery and the varied drugs release behaviors from two membranes (rapid drug release of the outer hydrogel, and prolonged drug release of the inner hydrogel).
26925765	0	71	from	Drugs	112:116	arg1	Hydrogels					30:38	Biocompatible Double-Membrane Hydrogels	0:38	Biocompatible Double-Membrane Hydrogels from Cationic Cellulose Nanocrystals and Anionic Alginate as Complexing Drugs	0:116	Biocompatible Double-Membrane Hydrogels from Cationic Cellulose Nanocrystals and Anionic Alginate as Complexing Drugs Codelivery.
26925765	0	72	dep	Codelivery	118:127	arg1	Hydrogels					30:38	Biocompatible Double-Membrane Hydrogels	0:38	Biocompatible Double-Membrane Hydrogels from Cationic Cellulose Nanocrystals and Anionic Alginate as Complexing Drugs	0:116	Biocompatible Double-Membrane Hydrogels from Cationic Cellulose Nanocrystals and Anionic Alginate as Complexing Drugs Codelivery.
26925765	4	73	theme	hydrogel	963:970	arg1	membranes					920:928	two membranes	916:928	two membranes (rapid drug release of the outer hydrogel, and prolonged drug release of the inner hydrogel)	916:1021	Two drugs are introduced into the different membranes of the hydrogel, which will ensure the complexing drugs codelivery and the varied drugs release behaviors from two membranes (rapid drug release of the outer hydrogel, and prolonged drug release of the inner hydrogel).
26925765	4	73	theme	hydrogel	963:970	arg1	release					942:948	rapid drug release	931:948	rapid drug release of the outer hydrogel	931:970	Two drugs are introduced into the different membranes of the hydrogel, which will ensure the complexing drugs codelivery and the varied drugs release behaviors from two membranes (rapid drug release of the outer hydrogel, and prolonged drug release of the inner hydrogel).
26925765	4	73	theme	hydrogel	963:970	arg1	release					992:998	prolonged drug release	977:998	prolonged drug release of the inner hydrogel	977:1020	Two drugs are introduced into the different membranes of the hydrogel, which will ensure the complexing drugs codelivery and the varied drugs release behaviors from two membranes (rapid drug release of the outer hydrogel, and prolonged drug release of the inner hydrogel).
26925765	6	74	theme	drug	1711:1714	arg1	resistance					1716:1725	drug resistance	1711:1725	drug resistance	1711:1725	Derived from natural polysaccharides (cellulose and alginate), the novel double-membrane structure hydrogel material developed in this study is biocompatible and can realize the complexing drugs release with the first quick release of one drug and the successively slow release of another drug, which is expected to achieve the synergistic release effects or potentially provide the solution to drug resistance in biomedical application.
26925765	0	75	theme	Double-Membrane	14:28	arg1	Hydrogels					30:38	Biocompatible Double-Membrane Hydrogels	0:38	Biocompatible Double-Membrane Hydrogels from Cationic Cellulose Nanocrystals and Anionic Alginate as Complexing Drugs	0:116	Biocompatible Double-Membrane Hydrogels from Cationic Cellulose Nanocrystals and Anionic Alginate as Complexing Drugs Codelivery.
26925765	6	76	theme	drug	1605:1608	arg1	release					1540:1546	the first quick release	1524:1546	the first quick release of one drug	1524:1558	Derived from natural polysaccharides (cellulose and alginate), the novel double-membrane structure hydrogel material developed in this study is biocompatible and can realize the complexing drugs release with the first quick release of one drug and the successively slow release of another drug, which is expected to achieve the synergistic release effects or potentially provide the solution to drug resistance in biomedical application.
26925765	6	76	theme	drug	1605:1608	arg1	release					1586:1592	the successively slow release	1564:1592	the successively slow release of another drug, which is expected to achieve the synergistic release effects or potentially provide the solution to drug resistance in biomedical application	1564:1751	Derived from natural polysaccharides (cellulose and alginate), the novel double-membrane structure hydrogel material developed in this study is biocompatible and can realize the complexing drugs release with the first quick release of one drug and the successively slow release of another drug, which is expected to achieve the synergistic release effects or potentially provide the solution to drug resistance in biomedical application.
26925765	3	77	theme	layer	520:524	arg1	thickness					497:505	The thickness	493:505	The thickness of the outer layer	493:524	The thickness of the outer layer can be regulated by the adsorption duration of neat alginate, and the shape of the inner layer can directly determine the morphology and dimensions of the double-membrane hydrogel (microsphere, capsule, and filmlike shapes).
26925765	0	78	theme	Cellulose	54:62	arg1	Nanocrystals					64:75	Cationic Cellulose Nanocrystals	45:75	Cationic Cellulose Nanocrystals	45:75	Biocompatible Double-Membrane Hydrogels from Cationic Cellulose Nanocrystals and Anionic Alginate as Complexing Drugs Codelivery.
26925765	2	79	theme	cationic	458:465	arg1	CNC					467:469	cationic CNC	458:469	cationic CNC	458:469	The architecture of the double-membrane hydrogel involves an external membrane composed of neat alginate, and an internal composite hydrogel consolidates by electrostatic interactions between cationic CNC and anionic alginate.
26925765	6	80	theme	structure	1405:1413	arg1	material					1424:1431	the novel double-membrane structure hydrogel material	1379:1431	the novel double-membrane structure hydrogel material developed in this study	1379:1455	Derived from natural polysaccharides (cellulose and alginate), the novel double-membrane structure hydrogel material developed in this study is biocompatible and can realize the complexing drugs release with the first quick release of one drug and the successively slow release of another drug, which is expected to achieve the synergistic release effects or potentially provide the solution to drug resistance in biomedical application.
26925765	6	80	theme	structure	1405:1413	arg1	biocompatible					1460:1472	biocompatible	1460:1472	biocompatible	1460:1472	Derived from natural polysaccharides (cellulose and alginate), the novel double-membrane structure hydrogel material developed in this study is biocompatible and can realize the complexing drugs release with the first quick release of one drug and the successively slow release of another drug, which is expected to achieve the synergistic release effects or potentially provide the solution to drug resistance in biomedical application.
26925765	4	81	theme	inner	1007:1011	arg1	hydrogel					1013:1020	the inner hydrogel	1003:1020	the inner hydrogel	1003:1020	Two drugs are introduced into the different membranes of the hydrogel, which will ensure the complexing drugs codelivery and the varied drugs release behaviors from two membranes (rapid drug release of the outer hydrogel, and prolonged drug release of the inner hydrogel).
26925765	6	82	theme	drugs	1505:1509	arg1	release					1511:1517	the complexing drugs release	1490:1517	the complexing drugs release	1490:1517	Derived from natural polysaccharides (cellulose and alginate), the novel double-membrane structure hydrogel material developed in this study is biocompatible and can realize the complexing drugs release with the first quick release of one drug and the successively slow release of another drug, which is expected to achieve the synergistic release effects or potentially provide the solution to drug resistance in biomedical application.
26925765	5	83	theme	drug	1175:1178	arg1	release					1180:1186	the sustained drug release	1161:1186	the sustained drug release ascribed to the "nano-obstruction effect" and "nanolocking effect" induced by the presence of CCNC components in the hydrogels	1161:1313	The double-membrane hydrogel containing the chemically modified cellulose nanocrystals (CCNC) in the inner membrane hydrogel can provide the sustained drug release ascribed to the "nano-obstruction effect" and "nanolocking effect" induced by the presence of CCNC components in the hydrogels.
26925765	3	84	theme	hydrogel	697:704	arg1	dimensions					663:672	dimensions	663:672	dimensions	663:672	The thickness of the outer layer can be regulated by the adsorption duration of neat alginate, and the shape of the inner layer can directly determine the morphology and dimensions of the double-membrane hydrogel (microsphere, capsule, and filmlike shapes).
26925765	3	84	theme	hydrogel	697:704	arg1	morphology					648:657	morphology	648:657	morphology	648:657	The thickness of the outer layer can be regulated by the adsorption duration of neat alginate, and the shape of the inner layer can directly determine the morphology and dimensions of the double-membrane hydrogel (microsphere, capsule, and filmlike shapes).
26925765	5	85	theme	effect	1247:1252	arg1	"					1253:1253	"nanolocking effect"	1234:1253	"nanolocking effect"	1234:1253	The double-membrane hydrogel containing the chemically modified cellulose nanocrystals (CCNC) in the inner membrane hydrogel can provide the sustained drug release ascribed to the "nano-obstruction effect" and "nanolocking effect" induced by the presence of CCNC components in the hydrogels.
25407786	5	0	theme	biodegradation	841:854	arg1	rate					856:859	the low biodegradation rate	833:859	the low biodegradation rate of PCL	833:866	PCL can adjust humidity sensitivity of starch as a biomaterial; while starch can enhance the low biodegradation rate of PCL.
25407786	4	1	theme	starch	541:546	arg1	blends					627:632	starch (a natural, hydrophilic, stiff, abundant polymer with a high degradation rate) blends	541:632	starch (a natural, hydrophilic, stiff, abundant polymer with a high degradation rate) blends	541:632	The properties of PCL (a synthetic, hydrophobic, flexible, expensive polymer with a low degradation rate) and starch (a natural, hydrophilic, stiff, abundant polymer with a high degradation rate) blends are interesting because of the composite components have completely different structures and characteristics.
25407786	6	2	theme	degradation	1046:1056	arg1	terms					1011:1015	terms	1011:1015	terms of mechanical properties and degradation which make it suitable for many biomedical applications	1011:1112	Thus, by appropriate blending, SPCL can overcome important limitations of both PCL and starch components and promote controllable behavior in terms of mechanical properties and degradation which make it suitable for many biomedical applications.
25407786	4	3	theme	blends	627:632	arg1	properties					435:444	The properties	431:444	The properties of PCL (a synthetic, hydrophobic, flexible, expensive polymer with a low degradation rate) and starch (a natural, hydrophilic, stiff, abundant polymer with a high degradation rate) blends	431:632	The properties of PCL (a synthetic, hydrophobic, flexible, expensive polymer with a low degradation rate) and starch (a natural, hydrophilic, stiff, abundant polymer with a high degradation rate) blends are interesting because of the composite components have completely different structures and characteristics.
25407786	4	3	theme	blends	627:632	arg1	interesting					638:648	interesting	638:648	interesting	638:648	The properties of PCL (a synthetic, hydrophobic, flexible, expensive polymer with a low degradation rate) and starch (a natural, hydrophilic, stiff, abundant polymer with a high degradation rate) blends are interesting because of the composite components have completely different structures and characteristics.
25407786	6	4	theme	many	1085:1088	arg1	applications					1101:1112	many biomedical applications	1085:1112	many biomedical applications	1085:1112	Thus, by appropriate blending, SPCL can overcome important limitations of both PCL and starch components and promote controllable behavior in terms of mechanical properties and degradation which make it suitable for many biomedical applications.
25407786	7	5	theme	modification	1167:1178	arg1	physical					1252:1259	physical	1252:1259	physical	1252:1259	This article reviewed the different fabrication and modification methods of the SPCL composite; different properties such as structural, physical, and chemical as well as degradation behavior; and different applications as biomaterials.
25407786	7	5	theme	modification	1167:1178	arg1	chemical					1266:1273	chemical	1266:1273	chemical	1266:1273	This article reviewed the different fabrication and modification methods of the SPCL composite; different properties such as structural, physical, and chemical as well as degradation behavior; and different applications as biomaterials.
25407786	7	5	theme	modification	1167:1178	arg1	structural					1240:1249	structural	1240:1249	structural	1240:1249	This article reviewed the different fabrication and modification methods of the SPCL composite; different properties such as structural, physical, and chemical as well as degradation behavior; and different applications as biomaterials.
25407786	7	5	theme	modification	1167:1178	arg1	behavior					1298:1305	degradation behavior	1286:1305	structural, physical, and chemical as well as degradation behavior	1240:1305	This article reviewed the different fabrication and modification methods of the SPCL composite; different properties such as structural, physical, and chemical as well as degradation behavior; and different applications as biomaterials.
25407786	7	5	theme	modification	1167:1178	arg1	methods					1180:1186	modification methods	1167:1186	modification methods	1167:1186	This article reviewed the different fabrication and modification methods of the SPCL composite; different properties such as structural, physical, and chemical as well as degradation behavior; and different applications as biomaterials.
25407786	4	6	dep	blends	627:632	arg1	polymer					589:595	a natural, hydrophilic, stiff, abundant polymer	549:595	a natural, hydrophilic, stiff, abundant polymer with a high degradation rate	549:624	The properties of PCL (a synthetic, hydrophobic, flexible, expensive polymer with a low degradation rate) and starch (a natural, hydrophilic, stiff, abundant polymer with a high degradation rate) blends are interesting because of the composite components have completely different structures and characteristics.
25407786	4	7	theme	different	702:710	arg1	structures					712:721	completely different structures	691:721	completely different structures	691:721	The properties of PCL (a synthetic, hydrophobic, flexible, expensive polymer with a low degradation rate) and starch (a natural, hydrophilic, stiff, abundant polymer with a high degradation rate) blends are interesting because of the composite components have completely different structures and characteristics.
25407786	6	8	theme	important	918:926	arg1	limitations					928:938	important limitations	918:938	important limitations of both PCL and starch components	918:972	Thus, by appropriate blending, SPCL can overcome important limitations of both PCL and starch components and promote controllable behavior in terms of mechanical properties and degradation which make it suitable for many biomedical applications.
25407786	7	9	theme	different	1141:1149	arg1	fabrication					1151:1161	different fabrication	1141:1161	different fabrication	1141:1161	This article reviewed the different fabrication and modification methods of the SPCL composite; different properties such as structural, physical, and chemical as well as degradation behavior; and different applications as biomaterials.
25407786	4	10	theme	characteristics	727:741	arg1	properties					435:444	The properties	431:444	The properties of PCL (a synthetic, hydrophobic, flexible, expensive polymer with a low degradation rate) and starch (a natural, hydrophilic, stiff, abundant polymer with a high degradation rate) blends	431:632	The properties of PCL (a synthetic, hydrophobic, flexible, expensive polymer with a low degradation rate) and starch (a natural, hydrophilic, stiff, abundant polymer with a high degradation rate) blends are interesting because of the composite components have completely different structures and characteristics.
25407786	4	10	theme	characteristics	727:741	arg1	interesting					638:648	interesting	638:648	interesting	638:648	The properties of PCL (a synthetic, hydrophobic, flexible, expensive polymer with a low degradation rate) and starch (a natural, hydrophilic, stiff, abundant polymer with a high degradation rate) blends are interesting because of the composite components have completely different structures and characteristics.
25407786	6	11	theme	mechanical	1020:1029	arg1	properties					1031:1040	mechanical properties	1020:1040	mechanical properties	1020:1040	Thus, by appropriate blending, SPCL can overcome important limitations of both PCL and starch components and promote controllable behavior in terms of mechanical properties and degradation which make it suitable for many biomedical applications.
25407786	3	12	theme	first	338:342	arg1	Novamont					322:329	Novamont	322:329	Novamont	322:329	Novamont is the first company that manufactured a PCL/starch (SPCL) composite under the trademark Mater-Bi®.
25407786	3	12	theme	first	338:342	arg1	company					344:350	the first company that manufactured a PCL/starch (SPCL) composite under the trademark Mater-Bi®	334:428	the first company that manufactured a PCL/starch (SPCL) composite under the trademark Mater-Bi®	334:428	Novamont is the first company that manufactured a PCL/starch (SPCL) composite under the trademark Mater-Bi®.
25407786	4	13	dep	PCL	449:451	arg1	polymer					500:506	a synthetic, hydrophobic, flexible, expensive polymer	454:506	a synthetic, hydrophobic, flexible, expensive polymer with a low degradation rate	454:534	The properties of PCL (a synthetic, hydrophobic, flexible, expensive polymer with a low degradation rate) and starch (a natural, hydrophilic, stiff, abundant polymer with a high degradation rate) blends are interesting because of the composite components have completely different structures and characteristics.
25407786	6	14	theme	starch	956:961	arg1	components					963:972	starch components	956:972	starch components	956:972	Thus, by appropriate blending, SPCL can overcome important limitations of both PCL and starch components and promote controllable behavior in terms of mechanical properties and degradation which make it suitable for many biomedical applications.
25407786	6	15	theme	properties	1031:1040	arg1	terms					1011:1015	terms	1011:1015	terms of mechanical properties and degradation which make it suitable for many biomedical applications	1011:1112	Thus, by appropriate blending, SPCL can overcome important limitations of both PCL and starch components and promote controllable behavior in terms of mechanical properties and degradation which make it suitable for many biomedical applications.
25407786	7	16	theme	degradation	1286:1296	arg1	behavior					1298:1305	degradation behavior	1286:1305	structural, physical, and chemical as well as degradation behavior	1240:1305	This article reviewed the different fabrication and modification methods of the SPCL composite; different properties such as structural, physical, and chemical as well as degradation behavior; and different applications as biomaterials.
25407786	3	17	theme	PCL/starch	372:381	arg1	composite					390:398	a PCL/starch (SPCL) composite	370:398	a PCL/starch (SPCL) composite	370:398	Novamont is the first company that manufactured a PCL/starch (SPCL) composite under the trademark Mater-Bi®.
25407786	4	18	theme	synthetic	456:464	arg1	polymer					500:506	a synthetic, hydrophobic, flexible, expensive polymer	454:506	a synthetic, hydrophobic, flexible, expensive polymer with a low degradation rate	454:534	The properties of PCL (a synthetic, hydrophobic, flexible, expensive polymer with a low degradation rate) and starch (a natural, hydrophilic, stiff, abundant polymer with a high degradation rate) blends are interesting because of the composite components have completely different structures and characteristics.
25407786	4	19	theme	components	675:684	arg1	properties					435:444	The properties	431:444	The properties of PCL (a synthetic, hydrophobic, flexible, expensive polymer with a low degradation rate) and starch (a natural, hydrophilic, stiff, abundant polymer with a high degradation rate) blends	431:632	The properties of PCL (a synthetic, hydrophobic, flexible, expensive polymer with a low degradation rate) and starch (a natural, hydrophilic, stiff, abundant polymer with a high degradation rate) blends are interesting because of the composite components have completely different structures and characteristics.
25407786	4	19	theme	components	675:684	arg1	interesting					638:648	interesting	638:648	interesting	638:648	The properties of PCL (a synthetic, hydrophobic, flexible, expensive polymer with a low degradation rate) and starch (a natural, hydrophilic, stiff, abundant polymer with a high degradation rate) blends are interesting because of the composite components have completely different structures and characteristics.
25407786	4	20	theme	degradation	609:619	arg1	rate					621:624	a high degradation rate	602:624	a high degradation rate	602:624	The properties of PCL (a synthetic, hydrophobic, flexible, expensive polymer with a low degradation rate) and starch (a natural, hydrophilic, stiff, abundant polymer with a high degradation rate) blends are interesting because of the composite components have completely different structures and characteristics.
25407786	0	21	theme	Polycaprolactone/starch	0:22	arg1	composite					24:32	Polycaprolactone/starch composite	0:32	Polycaprolactone/starch composite: Fabrication, structure, properties, and applications.	0:87	Polycaprolactone/starch composite: Fabrication, structure, properties, and applications.
25407786	4	22	theme	degradation	519:529	arg1	rate					531:534	a low degradation rate	513:534	a low degradation rate	513:534	The properties of PCL (a synthetic, hydrophobic, flexible, expensive polymer with a low degradation rate) and starch (a natural, hydrophilic, stiff, abundant polymer with a high degradation rate) blends are interesting because of the composite components have completely different structures and characteristics.
25407786	4	23	dep	components	675:684	arg1	have					686:689	have	686:689	have completely different structures	686:721	The properties of PCL (a synthetic, hydrophobic, flexible, expensive polymer with a low degradation rate) and starch (a natural, hydrophilic, stiff, abundant polymer with a high degradation rate) blends are interesting because of the composite components have completely different structures and characteristics.
25407786	6	24	from	behavior	999:1006	arg1	terms					1011:1015	terms	1011:1015	terms of mechanical properties and degradation which make it suitable for many biomedical applications	1011:1112	Thus, by appropriate blending, SPCL can overcome important limitations of both PCL and starch components and promote controllable behavior in terms of mechanical properties and degradation which make it suitable for many biomedical applications.
25407786	4	25	theme	composite	665:673	arg1	components					675:684	the composite components	661:684	the composite components have completely different structures	661:721	The properties of PCL (a synthetic, hydrophobic, flexible, expensive polymer with a low degradation rate) and starch (a natural, hydrophilic, stiff, abundant polymer with a high degradation rate) blends are interesting because of the composite components have completely different structures and characteristics.
25407786	3	26	theme	trademark	410:418	arg1	Mater-Bi®					420:428	the trademark Mater-Bi®	406:428	the trademark Mater-Bi®	406:428	Novamont is the first company that manufactured a PCL/starch (SPCL) composite under the trademark Mater-Bi®.
25407786	2	27	theme	polycaprolactone	250:265	arg1	blend					230:234	the blend	226:234	the blend of starch and polycaprolactone (PCL)	226:271	Among the different polymeric composites currently available, the blend of starch and polycaprolactone (PCL) has received the most attention since the 1980s.
25407786	4	28	with	polymer	500:506	arg1	rate					531:534	a low degradation rate	513:534	a low degradation rate	513:534	The properties of PCL (a synthetic, hydrophobic, flexible, expensive polymer with a low degradation rate) and starch (a natural, hydrophilic, stiff, abundant polymer with a high degradation rate) blends are interesting because of the composite components have completely different structures and characteristics.
25407786	5	29	theme	starch	783:788	arg1	biomaterial					795:805	biomaterial	795:805	biomaterial	795:805	PCL can adjust humidity sensitivity of starch as a biomaterial; while starch can enhance the low biodegradation rate of PCL.
25407786	5	29	theme	starch	783:788	arg1	sensitivity					768:778	humidity sensitivity	759:778	humidity sensitivity of starch	759:788	PCL can adjust humidity sensitivity of starch as a biomaterial; while starch can enhance the low biodegradation rate of PCL.
25407786	2	30	theme	polymeric	184:192	arg1	composites					194:203	the different polymeric composites	170:203	the different polymeric composites currently available	170:223	Among the different polymeric composites currently available, the blend of starch and polycaprolactone (PCL) has received the most attention since the 1980s.
25407786	2	31	theme	most	290:293	arg1	attention					295:303	the most attention	286:303	the most attention	286:303	Among the different polymeric composites currently available, the blend of starch and polycaprolactone (PCL) has received the most attention since the 1980s.
25407786	6	32	theme	controllable	986:997	arg1	behavior					999:1006	controllable behavior	986:1006	controllable behavior in terms of mechanical properties and degradation which make it suitable for many biomedical applications	986:1112	Thus, by appropriate blending, SPCL can overcome important limitations of both PCL and starch components and promote controllable behavior in terms of mechanical properties and degradation which make it suitable for many biomedical applications.
25407786	5	33	theme	PCL	864:866	arg1	rate					856:859	the low biodegradation rate	833:859	the low biodegradation rate of PCL	833:866	PCL can adjust humidity sensitivity of starch as a biomaterial; while starch can enhance the low biodegradation rate of PCL.
25407786	2	34	theme	different	174:182	arg1	composites					194:203	the different polymeric composites	170:203	the different polymeric composites currently available	170:223	Among the different polymeric composites currently available, the blend of starch and polycaprolactone (PCL) has received the most attention since the 1980s.
25407786	2	35	theme	starch	239:244	arg1	blend					230:234	the blend	226:234	the blend of starch and polycaprolactone (PCL)	226:271	Among the different polymeric composites currently available, the blend of starch and polycaprolactone (PCL) has received the most attention since the 1980s.
25407786	4	36	theme	high	604:607	arg1	rate					621:624	a high degradation rate	602:624	a high degradation rate	602:624	The properties of PCL (a synthetic, hydrophobic, flexible, expensive polymer with a low degradation rate) and starch (a natural, hydrophilic, stiff, abundant polymer with a high degradation rate) blends are interesting because of the composite components have completely different structures and characteristics.
25407786	4	37	theme	PCL	449:451	arg1	properties					435:444	The properties	431:444	The properties of PCL (a synthetic, hydrophobic, flexible, expensive polymer with a low degradation rate) and starch (a natural, hydrophilic, stiff, abundant polymer with a high degradation rate) blends	431:632	The properties of PCL (a synthetic, hydrophobic, flexible, expensive polymer with a low degradation rate) and starch (a natural, hydrophilic, stiff, abundant polymer with a high degradation rate) blends are interesting because of the composite components have completely different structures and characteristics.
25407786	4	37	theme	PCL	449:451	arg1	interesting					638:648	interesting	638:648	interesting	638:648	The properties of PCL (a synthetic, hydrophobic, flexible, expensive polymer with a low degradation rate) and starch (a natural, hydrophilic, stiff, abundant polymer with a high degradation rate) blends are interesting because of the composite components have completely different structures and characteristics.
25407786	4	38	dep	synthetic	456:464	arg1	expensive					490:498	expensive	490:498	expensive	490:498	The properties of PCL (a synthetic, hydrophobic, flexible, expensive polymer with a low degradation rate) and starch (a natural, hydrophilic, stiff, abundant polymer with a high degradation rate) blends are interesting because of the composite components have completely different structures and characteristics.
25407786	4	38	dep	synthetic	456:464	arg1	flexible					480:487	flexible	480:487	flexible	480:487	The properties of PCL (a synthetic, hydrophobic, flexible, expensive polymer with a low degradation rate) and starch (a natural, hydrophilic, stiff, abundant polymer with a high degradation rate) blends are interesting because of the composite components have completely different structures and characteristics.
25407786	4	38	dep	synthetic	456:464	arg1	hydrophobic					467:477	hydrophobic	467:477	hydrophobic	467:477	The properties of PCL (a synthetic, hydrophobic, flexible, expensive polymer with a low degradation rate) and starch (a natural, hydrophilic, stiff, abundant polymer with a high degradation rate) blends are interesting because of the composite components have completely different structures and characteristics.
25407786	3	39	theme	SPCL	384:387	arg1	composite					390:398	a PCL/starch (SPCL) composite	370:398	a PCL/starch (SPCL) composite	370:398	Novamont is the first company that manufactured a PCL/starch (SPCL) composite under the trademark Mater-Bi®.
25407786	6	40	theme	appropriate	878:888	arg1	blending					890:897	appropriate blending	878:897	appropriate blending	878:897	Thus, by appropriate blending, SPCL can overcome important limitations of both PCL and starch components and promote controllable behavior in terms of mechanical properties and degradation which make it suitable for many biomedical applications.
25407786	4	41	theme	low	515:517	arg1	rate					531:534	a low degradation rate	513:534	a low degradation rate	513:534	The properties of PCL (a synthetic, hydrophobic, flexible, expensive polymer with a low degradation rate) and starch (a natural, hydrophilic, stiff, abundant polymer with a high degradation rate) blends are interesting because of the composite components have completely different structures and characteristics.
25407786	7	42	dep	fabrication	1151:1161	arg1	the					1137:1139	the	1137:1139	the	1137:1139	This article reviewed the different fabrication and modification methods of the SPCL composite; different properties such as structural, physical, and chemical as well as degradation behavior; and different applications as biomaterials.
25407786	4	43	with	polymer	589:595	arg1	rate					621:624	a high degradation rate	602:624	a high degradation rate	602:624	The properties of PCL (a synthetic, hydrophobic, flexible, expensive polymer with a low degradation rate) and starch (a natural, hydrophilic, stiff, abundant polymer with a high degradation rate) blends are interesting because of the composite components have completely different structures and characteristics.
25407786	1	44	theme	biodegradable	113:125	arg1	polymers					127:134	biodegradable polymers	113:134	biodegradable polymers	113:134	Interests in the use of biodegradable polymers as biomaterials have grown.
25407786	2	45	theme	available	215:223	arg1	composites					194:203	the different polymeric composites	170:203	the different polymeric composites currently available	170:223	Among the different polymeric composites currently available, the blend of starch and polycaprolactone (PCL) has received the most attention since the 1980s.
25407786	1	46	theme	polymers	127:134	arg1	use					106:108	the use	102:108	the use of biodegradable polymers as biomaterials	102:150	Interests in the use of biodegradable polymers as biomaterials have grown.
25407786	6	47	theme	biomedical	1090:1099	arg1	applications					1101:1112	many biomedical applications	1085:1112	many biomedical applications	1085:1112	Thus, by appropriate blending, SPCL can overcome important limitations of both PCL and starch components and promote controllable behavior in terms of mechanical properties and degradation which make it suitable for many biomedical applications.
25407786	6	48	theme	components	963:972	arg1	limitations					928:938	important limitations	918:938	important limitations of both PCL and starch components	918:972	Thus, by appropriate blending, SPCL can overcome important limitations of both PCL and starch components and promote controllable behavior in terms of mechanical properties and degradation which make it suitable for many biomedical applications.
25407786	7	49	theme	different	1312:1320	arg1	physical					1252:1259	physical	1252:1259	physical	1252:1259	This article reviewed the different fabrication and modification methods of the SPCL composite; different properties such as structural, physical, and chemical as well as degradation behavior; and different applications as biomaterials.
25407786	7	49	theme	different	1312:1320	arg1	chemical					1266:1273	chemical	1266:1273	chemical	1266:1273	This article reviewed the different fabrication and modification methods of the SPCL composite; different properties such as structural, physical, and chemical as well as degradation behavior; and different applications as biomaterials.
25407786	7	49	theme	different	1312:1320	arg1	structural					1240:1249	structural	1240:1249	structural	1240:1249	This article reviewed the different fabrication and modification methods of the SPCL composite; different properties such as structural, physical, and chemical as well as degradation behavior; and different applications as biomaterials.
25407786	7	49	theme	different	1312:1320	arg1	behavior					1298:1305	degradation behavior	1286:1305	structural, physical, and chemical as well as degradation behavior	1240:1305	This article reviewed the different fabrication and modification methods of the SPCL composite; different properties such as structural, physical, and chemical as well as degradation behavior; and different applications as biomaterials.
25407786	7	49	theme	different	1312:1320	arg1	applications					1322:1333	different applications	1312:1333	different applications as biomaterials	1312:1349	This article reviewed the different fabrication and modification methods of the SPCL composite; different properties such as structural, physical, and chemical as well as degradation behavior; and different applications as biomaterials.
25407786	5	50	theme	low	837:839	arg1	rate					856:859	the low biodegradation rate	833:859	the low biodegradation rate of PCL	833:866	PCL can adjust humidity sensitivity of starch as a biomaterial; while starch can enhance the low biodegradation rate of PCL.
25407786	1	51	from	Interests	89:97	arg1	use					106:108	the use	102:108	the use of biodegradable polymers as biomaterials	102:150	Interests in the use of biodegradable polymers as biomaterials have grown.
25407786	4	52	theme	natural	551:557	arg1	polymer					589:595	a natural, hydrophilic, stiff, abundant polymer	549:595	a natural, hydrophilic, stiff, abundant polymer with a high degradation rate	549:624	The properties of PCL (a synthetic, hydrophobic, flexible, expensive polymer with a low degradation rate) and starch (a natural, hydrophilic, stiff, abundant polymer with a high degradation rate) blends are interesting because of the composite components have completely different structures and characteristics.
25407786	4	53	dep	natural	551:557	arg1	abundant					580:587	abundant	580:587	abundant	580:587	The properties of PCL (a synthetic, hydrophobic, flexible, expensive polymer with a low degradation rate) and starch (a natural, hydrophilic, stiff, abundant polymer with a high degradation rate) blends are interesting because of the composite components have completely different structures and characteristics.
25407786	4	53	dep	natural	551:557	arg1	stiff					573:577	stiff	573:577	stiff	573:577	The properties of PCL (a synthetic, hydrophobic, flexible, expensive polymer with a low degradation rate) and starch (a natural, hydrophilic, stiff, abundant polymer with a high degradation rate) blends are interesting because of the composite components have completely different structures and characteristics.
25407786	4	53	dep	natural	551:557	arg1	hydrophilic					560:570	hydrophilic	560:570	hydrophilic	560:570	The properties of PCL (a synthetic, hydrophobic, flexible, expensive polymer with a low degradation rate) and starch (a natural, hydrophilic, stiff, abundant polymer with a high degradation rate) blends are interesting because of the composite components have completely different structures and characteristics.
25407786	0	54	dep	composite	24:32	arg1	applications					75:86	applications	75:86	applications	75:86	Polycaprolactone/starch composite: Fabrication, structure, properties, and applications.
25407786	0	54	dep	composite	24:32	arg1	structure					48:56	structure	48:56	structure	48:56	Polycaprolactone/starch composite: Fabrication, structure, properties, and applications.
25407786	0	54	dep	composite	24:32	arg1	Fabrication					35:45	Fabrication	35:45	Fabrication	35:45	Polycaprolactone/starch composite: Fabrication, structure, properties, and applications.
25407786	0	54	dep	composite	24:32	arg1	properties					59:68	properties	59:68	properties	59:68	Polycaprolactone/starch composite: Fabrication, structure, properties, and applications.
25407786	7	55	theme	different	1211:1219	arg1	chemical					1266:1273	chemical	1266:1273	chemical	1266:1273	This article reviewed the different fabrication and modification methods of the SPCL composite; different properties such as structural, physical, and chemical as well as degradation behavior; and different applications as biomaterials.
25407786	7	55	theme	different	1211:1219	arg1	physical					1252:1259	physical	1252:1259	physical	1252:1259	This article reviewed the different fabrication and modification methods of the SPCL composite; different properties such as structural, physical, and chemical as well as degradation behavior; and different applications as biomaterials.
25407786	7	55	theme	different	1211:1219	arg1	properties					1221:1230	different properties	1211:1230	different properties such as structural, physical, and chemical as well as degradation behavior	1211:1305	This article reviewed the different fabrication and modification methods of the SPCL composite; different properties such as structural, physical, and chemical as well as degradation behavior; and different applications as biomaterials.
25407786	7	55	theme	different	1211:1219	arg1	structural					1240:1249	structural	1240:1249	structural	1240:1249	This article reviewed the different fabrication and modification methods of the SPCL composite; different properties such as structural, physical, and chemical as well as degradation behavior; and different applications as biomaterials.
25407786	7	55	theme	different	1211:1219	arg1	behavior					1298:1305	degradation behavior	1286:1305	structural, physical, and chemical as well as degradation behavior	1240:1305	This article reviewed the different fabrication and modification methods of the SPCL composite; different properties such as structural, physical, and chemical as well as degradation behavior; and different applications as biomaterials.
25407786	7	56	theme	SPCL	1195:1198	arg1	composite					1200:1208	the SPCL composite	1191:1208	the SPCL composite	1191:1208	This article reviewed the different fabrication and modification methods of the SPCL composite; different properties such as structural, physical, and chemical as well as degradation behavior; and different applications as biomaterials.
25407786	5	57	theme	humidity	759:766	arg1	biomaterial					795:805	biomaterial	795:805	biomaterial	795:805	PCL can adjust humidity sensitivity of starch as a biomaterial; while starch can enhance the low biodegradation rate of PCL.
25407786	5	57	theme	humidity	759:766	arg1	sensitivity					768:778	humidity sensitivity	759:778	humidity sensitivity of starch	759:788	PCL can adjust humidity sensitivity of starch as a biomaterial; while starch can enhance the low biodegradation rate of PCL.
25407786	7	58	theme	composite	1200:1208	arg1	applications					1322:1333	different applications	1312:1333	different applications as biomaterials	1312:1349	This article reviewed the different fabrication and modification methods of the SPCL composite; different properties such as structural, physical, and chemical as well as degradation behavior; and different applications as biomaterials.
25407786	7	58	theme	composite	1200:1208	arg1	physical					1252:1259	physical	1252:1259	physical	1252:1259	This article reviewed the different fabrication and modification methods of the SPCL composite; different properties such as structural, physical, and chemical as well as degradation behavior; and different applications as biomaterials.
25407786	7	58	theme	composite	1200:1208	arg1	properties					1221:1230	different properties	1211:1230	different properties such as structural, physical, and chemical as well as degradation behavior	1211:1305	This article reviewed the different fabrication and modification methods of the SPCL composite; different properties such as structural, physical, and chemical as well as degradation behavior; and different applications as biomaterials.
25407786	7	58	theme	composite	1200:1208	arg1	methods					1180:1186	modification methods	1167:1186	modification methods	1167:1186	This article reviewed the different fabrication and modification methods of the SPCL composite; different properties such as structural, physical, and chemical as well as degradation behavior; and different applications as biomaterials.
25407786	7	58	theme	composite	1200:1208	arg1	behavior					1298:1305	degradation behavior	1286:1305	structural, physical, and chemical as well as degradation behavior	1240:1305	This article reviewed the different fabrication and modification methods of the SPCL composite; different properties such as structural, physical, and chemical as well as degradation behavior; and different applications as biomaterials.
25407786	7	58	theme	composite	1200:1208	arg1	chemical					1266:1273	chemical	1266:1273	chemical	1266:1273	This article reviewed the different fabrication and modification methods of the SPCL composite; different properties such as structural, physical, and chemical as well as degradation behavior; and different applications as biomaterials.
25407786	7	58	theme	composite	1200:1208	arg1	structural					1240:1249	structural	1240:1249	structural	1240:1249	This article reviewed the different fabrication and modification methods of the SPCL composite; different properties such as structural, physical, and chemical as well as degradation behavior; and different applications as biomaterials.
25407786	7	58	theme	composite	1200:1208	arg1	fabrication					1151:1161	different fabrication	1141:1161	different fabrication	1141:1161	This article reviewed the different fabrication and modification methods of the SPCL composite; different properties such as structural, physical, and chemical as well as degradation behavior; and different applications as biomaterials.
25407786	6	59	theme	PCL	948:950	arg1	limitations					928:938	important limitations	918:938	important limitations of both PCL and starch components	918:972	Thus, by appropriate blending, SPCL can overcome important limitations of both PCL and starch components and promote controllable behavior in terms of mechanical properties and degradation which make it suitable for many biomedical applications.
27492556	5	0	theme	mechanical	673:682	arg1	properties					684:693	mechanical properties	673:693	mechanical properties	673:693	seeds by an aqueous method, characterized for its compositional analysis (mannose: galactose ratio), physicochemical and functional properties (solubility), and mechanical properties.
27492556	2	1	from	applications	184:195	arg1	industry					228:235	the pharmaceutical and food industry	200:235	industry	228:235	Water soluble gums also known as hydrocolloids are increasingly finding applications in the pharmaceutical and food industry due to their versatile functional properties.
27492556	2	2	theme	soluble	118:124	arg1	gums					126:129	Water soluble gums	112:129	Water soluble gums also known as hydrocolloids	112:157	Water soluble gums also known as hydrocolloids are increasingly finding applications in the pharmaceutical and food industry due to their versatile functional properties.
27492556	2	2	theme	soluble	118:124	arg1	applications					184:195	finding applications	176:195	finding applications in the pharmaceutical and food industry due to their versatile functional properties	176:280	Water soluble gums also known as hydrocolloids are increasingly finding applications in the pharmaceutical and food industry due to their versatile functional properties.
27492556	2	3	theme	pharmaceutical	204:217	arg1	industry					228:235	the pharmaceutical and food industry	200:235	industry	228:235	Water soluble gums also known as hydrocolloids are increasingly finding applications in the pharmaceutical and food industry due to their versatile functional properties.
27492556	8	4	theme	Leucaena	973:980	arg1	leucocephala					982:993	Leucaena leucocephala galactomannan (LLG)	973:1013	Leucaena leucocephala galactomannan (LLG)	973:1013	The yield of Leucaena leucocephala galactomannan (LLG) was found to be 20% (w/w).
27492556	10	5	theme	physicochemical	1189:1203	arg1	characteristics					1220:1234	The physicochemical and functional characteristics	1185:1234	The physicochemical and functional characteristics of native and derivatized gum	1185:1264	The physicochemical and functional characteristics of native and derivatized gum suggest its potential role in food and pharmaceutical industries.
27492556	3	6	theme	gelling	382:388	arg1	agents					390:395	emulsifying, thickening and gelling agents	354:395	emulsifying, thickening and gelling agents	354:395	They possess considerable use in food and pharmaceutical industries as emulsifying, thickening and gelling agents.
27492556	5	7	theme	functional	633:642	arg1	solubility					656:665	solubility	656:665	solubility	656:665	seeds by an aqueous method, characterized for its compositional analysis (mannose: galactose ratio), physicochemical and functional properties (solubility), and mechanical properties.
27492556	5	7	theme	functional	633:642	arg1	properties					644:653	physicochemical and functional properties	613:653	physicochemical and functional properties (solubility)	613:666	seeds by an aqueous method, characterized for its compositional analysis (mannose: galactose ratio), physicochemical and functional properties (solubility), and mechanical properties.
27492556	6	8	theme	derivatization	789:802	arg1	method					775:780	the method	771:780	the method of its derivatization	771:802	The extracted gum was derivatized to form its carboxymethyl derivative and the method of its derivatization was optimized by varying the reaction parameters.
27492556	10	9	theme	gum	1262:1264	arg1	characteristics					1220:1234	The physicochemical and functional characteristics	1185:1234	The physicochemical and functional characteristics of native and derivatized gum	1185:1264	The physicochemical and functional characteristics of native and derivatized gum suggest its potential role in food and pharmaceutical industries.
27492556	5	10	theme	aqueous	524:530	arg1	method					532:537	an aqueous method	521:537	an aqueous method	521:537	seeds by an aqueous method, characterized for its compositional analysis (mannose: galactose ratio), physicochemical and functional properties (solubility), and mechanical properties.
27492556	3	11	from	use	309:311	arg1	industries					340:349	food and pharmaceutical industries	316:349	food and pharmaceutical industries	316:349	They possess considerable use in food and pharmaceutical industries as emulsifying, thickening and gelling agents.
27492556	3	12	theme	thickening	367:376	arg1	agents					390:395	emulsifying, thickening and gelling agents	354:395	emulsifying, thickening and gelling agents	354:395	They possess considerable use in food and pharmaceutical industries as emulsifying, thickening and gelling agents.
27492556	6	13	theme	carboxymethyl	742:754	arg1	derivative					756:765	its carboxymethyl derivative	738:765	its carboxymethyl derivative	738:765	The extracted gum was derivatized to form its carboxymethyl derivative and the method of its derivatization was optimized by varying the reaction parameters.
27492556	4	14	theme	heteropolysaccharide	429:448	arg1	galactomannan					450:462	a heteropolysaccharide galactomannan	427:462	a heteropolysaccharide galactomannan	427:462	In the present investigation a heteropolysaccharide galactomannan was extracted from Leucaena leucocephala (Lam.)
27492556	10	15	theme	native	1239:1244	arg1	gum					1262:1264	native and derivatized gum	1239:1264	native and derivatized gum	1239:1264	The physicochemical and functional characteristics of native and derivatized gum suggest its potential role in food and pharmaceutical industries.
27492556	10	16	from	role	1288:1291	arg1	industries					1320:1329	food and pharmaceutical industries	1296:1329	food and pharmaceutical industries	1296:1329	The physicochemical and functional characteristics of native and derivatized gum suggest its potential role in food and pharmaceutical industries.
27492556	9	17	theme	optimized	1046:1054	arg1	NaOH					1152:1155	6.0g NaOH	1147:1155	6.0g NaOH	1147:1155	The optimized parameters for carboxymethylation reaction (degree of substitution 0.805) were found to be 6.0g NaOH, 10.0g MCA, at 60°C for 4h.
27492556	9	17	theme	optimized	1046:1054	arg1	parameters					1056:1065	The optimized parameters	1042:1065	The optimized parameters for carboxymethylation reaction (degree of substitution 0.805)	1042:1128	The optimized parameters for carboxymethylation reaction (degree of substitution 0.805) were found to be 6.0g NaOH, 10.0g MCA, at 60°C for 4h.
27492556	9	18	from	60°C	1172:1175	arg1	NaOH					1152:1155	6.0g NaOH	1147:1155	6.0g NaOH	1147:1155	The optimized parameters for carboxymethylation reaction (degree of substitution 0.805) were found to be 6.0g NaOH, 10.0g MCA, at 60°C for 4h.
27492556	9	18	from	60°C	1172:1175	arg1	parameters					1056:1065	The optimized parameters	1042:1065	The optimized parameters for carboxymethylation reaction (degree of substitution 0.805)	1042:1128	The optimized parameters for carboxymethylation reaction (degree of substitution 0.805) were found to be 6.0g NaOH, 10.0g MCA, at 60°C for 4h.
27492556	7	19	theme	elemental	935:943	arg1	analysis					945:952	elemental analysis	935:952	elemental analysis	935:952	The native and derivatized gum was characterized by FTIR, XRD, DSC, NMR, SEM and elemental analysis, etc.
27492556	4	20	theme	present	405:411	arg1	investigation					413:425	the present investigation	401:425	the present investigation	401:425	In the present investigation a heteropolysaccharide galactomannan was extracted from Leucaena leucocephala (Lam.)
27492556	3	21	contain	possess	288:294	arg1	They					283:286	They	283:286	They	283:286	They possess considerable use in food and pharmaceutical industries as emulsifying, thickening and gelling agents.
27492556	3	21	contain	possess	288:294	arg2	use					309:311	considerable use	296:311	considerable use in food and pharmaceutical industries as emulsifying, thickening and gelling agents	296:395	They possess considerable use in food and pharmaceutical industries as emulsifying, thickening and gelling agents.
27492556	3	22	theme	considerable	296:307	arg1	use					309:311	considerable use	296:311	considerable use in food and pharmaceutical industries as emulsifying, thickening and gelling agents	296:395	They possess considerable use in food and pharmaceutical industries as emulsifying, thickening and gelling agents.
27492556	5	23	theme	physicochemical	613:627	arg1	solubility					656:665	solubility	656:665	solubility	656:665	seeds by an aqueous method, characterized for its compositional analysis (mannose: galactose ratio), physicochemical and functional properties (solubility), and mechanical properties.
27492556	5	23	theme	physicochemical	613:627	arg1	properties					644:653	physicochemical and functional properties	613:653	physicochemical and functional properties (solubility)	613:666	seeds by an aqueous method, characterized for its compositional analysis (mannose: galactose ratio), physicochemical and functional properties (solubility), and mechanical properties.
27492556	9	24	theme	6.0g	1147:1150	arg1	NaOH					1152:1155	6.0g NaOH	1147:1155	6.0g NaOH	1147:1155	The optimized parameters for carboxymethylation reaction (degree of substitution 0.805) were found to be 6.0g NaOH, 10.0g MCA, at 60°C for 4h.
27492556	9	24	theme	6.0g	1147:1150	arg1	parameters					1056:1065	The optimized parameters	1042:1065	The optimized parameters for carboxymethylation reaction (degree of substitution 0.805)	1042:1128	The optimized parameters for carboxymethylation reaction (degree of substitution 0.805) were found to be 6.0g NaOH, 10.0g MCA, at 60°C for 4h.
27492556	8	25	dep	leucocephala	982:993	arg1	LLG					1010:1012	LLG	1010:1012	LLG	1010:1012	The yield of Leucaena leucocephala galactomannan (LLG) was found to be 20% (w/w).
27492556	8	25	dep	leucocephala	982:993	arg1	galactomannan					995:1007	galactomannan	995:1007	Leucaena leucocephala galactomannan (LLG)	973:1013	The yield of Leucaena leucocephala galactomannan (LLG) was found to be 20% (w/w).
27492556	10	26	theme	potential	1278:1286	arg1	role					1288:1291	its potential role	1274:1291	its potential role in food and pharmaceutical industries	1274:1329	The physicochemical and functional characteristics of native and derivatized gum suggest its potential role in food and pharmaceutical industries.
27492556	10	27	theme	derivatized	1250:1260	arg1	gum					1262:1264	native and derivatized gum	1239:1264	native and derivatized gum	1239:1264	The physicochemical and functional characteristics of native and derivatized gum suggest its potential role in food and pharmaceutical industries.
27492556	7	28	theme	native	858:863	arg1	gum					881:883	The native and derivatized gum	854:883	The native and derivatized gum	854:883	The native and derivatized gum was characterized by FTIR, XRD, DSC, NMR, SEM and elemental analysis, etc.
27492556	7	29	theme	derivatized	869:879	arg1	gum					881:883	The native and derivatized gum	854:883	The native and derivatized gum	854:883	The native and derivatized gum was characterized by FTIR, XRD, DSC, NMR, SEM and elemental analysis, etc.
27492556	0	30	theme	leucocephala	42:53	arg1	derivatization					15:28	derivatization	15:28	derivatization	15:28	Extraction and derivatization of Leucaena leucocephala (Lam.)
27492556	0	30	theme	leucocephala	42:53	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction and derivatization of Leucaena leucocephala (Lam.)
27492556	6	31	theme	extracted	700:708	arg1	gum					710:712	The extracted gum	696:712	The extracted gum	696:712	The extracted gum was derivatized to form its carboxymethyl derivative and the method of its derivatization was optimized by varying the reaction parameters.
27492556	9	32	theme	0.805	1123:1127	arg1	degree					1100:1105	degree	1100:1105	degree of substitution 0.805	1100:1127	The optimized parameters for carboxymethylation reaction (degree of substitution 0.805) were found to be 6.0g NaOH, 10.0g MCA, at 60°C for 4h.
27492556	0	33	theme	Leucaena	33:40	arg1	leucocephala					42:53	Leucaena leucocephala	33:53	Leucaena leucocephala	33:53	Extraction and derivatization of Leucaena leucocephala (Lam.)
27492556	2	34	theme	finding	176:182	arg1	applications					184:195	finding applications	176:195	finding applications in the pharmaceutical and food industry due to their versatile functional properties	176:280	Water soluble gums also known as hydrocolloids are increasingly finding applications in the pharmaceutical and food industry due to their versatile functional properties.
27492556	2	34	theme	finding	176:182	arg1	gums					126:129	Water soluble gums	112:129	Water soluble gums also known as hydrocolloids	112:157	Water soluble gums also known as hydrocolloids are increasingly finding applications in the pharmaceutical and food industry due to their versatile functional properties.
27492556	10	35	theme	food	1296:1299	arg1	industries					1320:1329	food and pharmaceutical industries	1296:1329	food and pharmaceutical industries	1296:1329	The physicochemical and functional characteristics of native and derivatized gum suggest its potential role in food and pharmaceutical industries.
27492556	2	36	theme	due	237:239	arg1	industry					228:235	the pharmaceutical and food industry	200:235	industry	228:235	Water soluble gums also known as hydrocolloids are increasingly finding applications in the pharmaceutical and food industry due to their versatile functional properties.
27492556	5	37	theme	mannose	586:592	arg1	ratio					605:609	mannose: galactose ratio	586:609	mannose: galactose ratio	586:609	seeds by an aqueous method, characterized for its compositional analysis (mannose: galactose ratio), physicochemical and functional properties (solubility), and mechanical properties.
27492556	5	37	theme	mannose	586:592	arg1	analysis					576:583	its compositional analysis	558:583	its compositional analysis (mannose: galactose ratio)	558:610	seeds by an aqueous method, characterized for its compositional analysis (mannose: galactose ratio), physicochemical and functional properties (solubility), and mechanical properties.
27492556	1	38	dep	galactomannan	62:74	arg1	characterization					94:109	characterization	94:109	characterization	94:109	galactomannan: Optimization and characterization.
27492556	1	38	dep	galactomannan	62:74	arg1	Optimization					77:88	Optimization	77:88	Optimization	77:88	galactomannan: Optimization and characterization.
27492556	3	39	theme	pharmaceutical	325:338	arg1	industries					340:349	food and pharmaceutical industries	316:349	food and pharmaceutical industries	316:349	They possess considerable use in food and pharmaceutical industries as emulsifying, thickening and gelling agents.
27492556	2	40	theme	food	223:226	arg1	industry					228:235	the pharmaceutical and food industry	200:235	industry	228:235	Water soluble gums also known as hydrocolloids are increasingly finding applications in the pharmaceutical and food industry due to their versatile functional properties.
27492556	9	41	theme	substitution	1110:1121	arg1	0.805					1123:1127	substitution 0.805	1110:1127	substitution 0.805	1110:1127	The optimized parameters for carboxymethylation reaction (degree of substitution 0.805) were found to be 6.0g NaOH, 10.0g MCA, at 60°C for 4h.
27492556	0	42	dep	Extraction	0:9	arg1	Lam					56:58	Lam	56:58	Lam.	56:59	Extraction and derivatization of Leucaena leucocephala (Lam.)
27492556	5	43	theme	galactose	595:603	arg1	ratio					605:609	mannose: galactose ratio	586:609	mannose: galactose ratio	586:609	seeds by an aqueous method, characterized for its compositional analysis (mannose: galactose ratio), physicochemical and functional properties (solubility), and mechanical properties.
27492556	5	43	theme	galactose	595:603	arg1	analysis					576:583	its compositional analysis	558:583	its compositional analysis (mannose: galactose ratio)	558:610	seeds by an aqueous method, characterized for its compositional analysis (mannose: galactose ratio), physicochemical and functional properties (solubility), and mechanical properties.
27492556	8	44	theme	leucocephala	982:993	arg1	%					1033:1033	20%	1031:1033	20% (w/w)	1031:1039	The yield of Leucaena leucocephala galactomannan (LLG) was found to be 20% (w/w).
27492556	8	44	theme	leucocephala	982:993	arg1	yield					964:968	The yield	960:968	The yield of Leucaena leucocephala galactomannan (LLG)	960:1013	The yield of Leucaena leucocephala galactomannan (LLG) was found to be 20% (w/w).
27492556	10	45	theme	pharmaceutical	1305:1318	arg1	industries					1320:1329	food and pharmaceutical industries	1296:1329	food and pharmaceutical industries	1296:1329	The physicochemical and functional characteristics of native and derivatized gum suggest its potential role in food and pharmaceutical industries.
27492556	3	46	theme	food	316:319	arg1	industries					340:349	food and pharmaceutical industries	316:349	food and pharmaceutical industries	316:349	They possess considerable use in food and pharmaceutical industries as emulsifying, thickening and gelling agents.
27492556	4	47	theme	Leucaena	483:490	arg1	leucocephala					492:503	Leucaena leucocephala	483:503	Leucaena leucocephala	483:503	In the present investigation a heteropolysaccharide galactomannan was extracted from Leucaena leucocephala (Lam.)
27492556	9	48	dep	reaction	1090:1097	arg1	degree					1100:1105	degree	1100:1105	degree of substitution 0.805	1100:1127	The optimized parameters for carboxymethylation reaction (degree of substitution 0.805) were found to be 6.0g NaOH, 10.0g MCA, at 60°C for 4h.
27492556	9	49	theme	carboxymethylation	1071:1088	arg1	reaction					1090:1097	carboxymethylation reaction	1071:1097	carboxymethylation reaction (degree of substitution 0.805)	1071:1128	The optimized parameters for carboxymethylation reaction (degree of substitution 0.805) were found to be 6.0g NaOH, 10.0g MCA, at 60°C for 4h.
27492556	6	50	theme	reaction	833:840	arg1	parameters					842:851	the reaction parameters	829:851	the reaction parameters	829:851	The extracted gum was derivatized to form its carboxymethyl derivative and the method of its derivatization was optimized by varying the reaction parameters.
27492556	3	51	theme	emulsifying	354:364	arg1	agents					390:395	emulsifying, thickening and gelling agents	354:395	emulsifying, thickening and gelling agents	354:395	They possess considerable use in food and pharmaceutical industries as emulsifying, thickening and gelling agents.
27492556	2	52	theme	functional	260:269	arg1	properties					271:280	their versatile functional properties	244:280	their versatile functional properties	244:280	Water soluble gums also known as hydrocolloids are increasingly finding applications in the pharmaceutical and food industry due to their versatile functional properties.
27492556	4	53	dep	extracted	468:476	arg1	Lam					506:508	Lam	506:508	Lam.	506:509	In the present investigation a heteropolysaccharide galactomannan was extracted from Leucaena leucocephala (Lam.)
27492556	2	54	theme	versatile	250:258	arg1	properties					271:280	their versatile functional properties	244:280	their versatile functional properties	244:280	Water soluble gums also known as hydrocolloids are increasingly finding applications in the pharmaceutical and food industry due to their versatile functional properties.
27492556	5	55	theme	compositional	562:574	arg1	ratio					605:609	mannose: galactose ratio	586:609	mannose: galactose ratio	586:609	seeds by an aqueous method, characterized for its compositional analysis (mannose: galactose ratio), physicochemical and functional properties (solubility), and mechanical properties.
27492556	5	55	theme	compositional	562:574	arg1	analysis					576:583	its compositional analysis	558:583	its compositional analysis (mannose: galactose ratio)	558:610	seeds by an aqueous method, characterized for its compositional analysis (mannose: galactose ratio), physicochemical and functional properties (solubility), and mechanical properties.
27492556	10	56	theme	functional	1209:1218	arg1	characteristics					1220:1234	The physicochemical and functional characteristics	1185:1234	The physicochemical and functional characteristics of native and derivatized gum	1185:1264	The physicochemical and functional characteristics of native and derivatized gum suggest its potential role in food and pharmaceutical industries.
24950308	1	0	theme	/montmorillonite	145:160	arg1	composites					171:180	chitosan-g-(N-vinyl pyrrolidone)/montmorillonite hydrogel composites	113:180	chitosan-g-(N-vinyl pyrrolidone)/montmorillonite hydrogel composites	113:180	A series of chitosan-g-(N-vinyl pyrrolidone)/montmorillonite hydrogel composites were synthesized by in situ intercalative polymerization.
24950308	7	1	theme	adsorption	897:906	arg1	capacity					908:915	the adsorption capacity	893:915	the adsorption capacity for the dye	893:927	The results show that the adsorption capacity for the dye increased with increase in dose, contact time but there was no change observed with respect to increase in temperature.
24950308	1	2	theme	hydrogel	162:169	arg1	composites					171:180	chitosan-g-(N-vinyl pyrrolidone)/montmorillonite hydrogel composites	113:180	chitosan-g-(N-vinyl pyrrolidone)/montmorillonite hydrogel composites	113:180	A series of chitosan-g-(N-vinyl pyrrolidone)/montmorillonite hydrogel composites were synthesized by in situ intercalative polymerization.
24950308	6	3	used	used	783:786	arg2	swelling					695:702	Maximum swelling	687:702	Maximum swelling	687:702	Maximum swelling was observed in the hydrogel containing low percentage of clay (H-0.2) and was used to investigate the adsorption of a water soluble cationic dye Rhodamine 6G (Rh6G).
24950308	5	4	theme	hydrogel	590:597	arg1	composites					599:608	the hydrogel composites	586:608	the hydrogel composites formed	586:615	The swelling of the hydrogel composites formed was found to be affected by the pH of the medium and the electrolyte.
24950308	3	5	with	sponge	422:427	arg1	structure					453:461	a homogeneous structure	439:461	a homogeneous structure	439:461	The morphologies of the hydrogel composites were sponge like with a homogeneous structure.
24950308	8	6	theme	Rh6G	1076:1079	arg1	kinetics					1064:1071	The adsorption kinetics	1049:1071	The adsorption kinetics of Rh6G	1049:1079	The adsorption kinetics of Rh6G followed Pseudo second order model and the equilibrium data was found to fit Freundlich model.
24950308	6	7	theme	Rhodamine	850:858	arg1	Rh6G					864:867	Rh6G	864:867	Rh6G	864:867	Maximum swelling was observed in the hydrogel containing low percentage of clay (H-0.2) and was used to investigate the adsorption of a water soluble cationic dye Rhodamine 6G (Rh6G).
24950308	6	7	theme	Rhodamine	850:858	arg1	6G					860:861	Rhodamine 6G	850:861	a water soluble cationic dye Rhodamine 6G (Rh6G)	821:868	Maximum swelling was observed in the hydrogel containing low percentage of clay (H-0.2) and was used to investigate the adsorption of a water soluble cationic dye Rhodamine 6G (Rh6G).
24950308	5	8	theme	composites	599:608	arg1	swelling					574:581	The swelling	570:581	The swelling of the hydrogel composites formed	570:615	The swelling of the hydrogel composites formed was found to be affected by the pH of the medium and the electrolyte.
24950308	4	9	theme	clay	479:482	arg1	effects					468:474	The effects	464:474	The effects of clay on the swelling, morphology, glass transition (Tg), and water structure	464:554	The effects of clay on the swelling, morphology, glass transition (Tg), and water structure were studied.
24950308	8	10	theme	second	1097:1102	arg1	model					1110:1114	Pseudo second order model	1090:1114	Pseudo second order model	1090:1114	The adsorption kinetics of Rh6G followed Pseudo second order model and the equilibrium data was found to fit Freundlich model.
24950308	6	11	theme	Maximum	687:693	arg1	swelling					695:702	Maximum swelling	687:702	Maximum swelling	687:702	Maximum swelling was observed in the hydrogel containing low percentage of clay (H-0.2) and was used to investigate the adsorption of a water soluble cationic dye Rhodamine 6G (Rh6G).
24950308	1	12	theme	composites	171:180	arg1	series					103:108	A series	101:108	A series of chitosan-g-(N-vinyl pyrrolidone)/montmorillonite hydrogel composites	101:180	A series of chitosan-g-(N-vinyl pyrrolidone)/montmorillonite hydrogel composites were synthesized by in situ intercalative polymerization.
24950308	8	13	theme	Pseudo	1090:1095	arg1	model					1110:1114	Pseudo second order model	1090:1114	Pseudo second order model	1090:1114	The adsorption kinetics of Rh6G followed Pseudo second order model and the equilibrium data was found to fit Freundlich model.
24950308	6	14	theme	cationic	837:844	arg1	dye					846:848	a water soluble cationic dye Rhodamine 6G (Rh6G)	821:868	a water soluble cationic dye Rhodamine 6G (Rh6G)	821:868	Maximum swelling was observed in the hydrogel containing low percentage of clay (H-0.2) and was used to investigate the adsorption of a water soluble cationic dye Rhodamine 6G (Rh6G).
24950308	8	15	theme	order	1104:1108	arg1	model					1110:1114	Pseudo second order model	1090:1114	Pseudo second order model	1090:1114	The adsorption kinetics of Rh6G followed Pseudo second order model and the equilibrium data was found to fit Freundlich model.
24950308	3	16	theme	composites	406:415	arg1	morphologies					377:388	The morphologies	373:388	The morphologies of the hydrogel composites	373:415	The morphologies of the hydrogel composites were sponge like with a homogeneous structure.
24950308	3	16	theme	composites	406:415	arg1	sponge					422:427	sponge	422:427	sponge	422:427	The morphologies of the hydrogel composites were sponge like with a homogeneous structure.
24950308	6	17	theme	soluble	829:835	arg1	dye					846:848	a water soluble cationic dye Rhodamine 6G (Rh6G)	821:868	a water soluble cationic dye Rhodamine 6G (Rh6G)	821:868	Maximum swelling was observed in the hydrogel containing low percentage of clay (H-0.2) and was used to investigate the adsorption of a water soluble cationic dye Rhodamine 6G (Rh6G).
24950308	6	18	theme	clay	762:765	arg1	percentage					748:757	the hydrogel containing low percentage	720:757	the hydrogel containing low percentage of clay (H-0.2)	720:773	Maximum swelling was observed in the hydrogel containing low percentage of clay (H-0.2) and was used to investigate the adsorption of a water soluble cationic dye Rhodamine 6G (Rh6G).
24950308	6	19	theme	dye	846:848	arg1	adsorption					807:816	the adsorption	803:816	the adsorption of a water soluble cationic dye Rhodamine 6G (Rh6G)	803:868	Maximum swelling was observed in the hydrogel containing low percentage of clay (H-0.2) and was used to investigate the adsorption of a water soluble cationic dye Rhodamine 6G (Rh6G).
24950308	3	20	theme	homogeneous	441:451	arg1	structure					453:461	a homogeneous structure	439:461	a homogeneous structure	439:461	The morphologies of the hydrogel composites were sponge like with a homogeneous structure.
24950308	0	21	theme	Adsorption	0:9	arg1	property					11:18	Adsorption property	0:18	Adsorption property of Rhodamine 6G onto chitosan-g-(N-vinyl pyrrolidone)	0:72	Adsorption property of Rhodamine 6G onto chitosan-g-(N-vinyl pyrrolidone)/montmorillonite composite.
24950308	2	22	theme	electron	314:321	arg1	microscopy					323:332	scanning electron microscopy	305:332	scanning electron microscopy	305:332	The properties of the composites were investigated by FTIR, XRD, scanning electron microscopy and differential scanning calorimetry.
24950308	8	23	theme	Freundlich	1158:1167	arg1	model					1169:1173	Freundlich model	1158:1173	Freundlich model	1158:1173	The adsorption kinetics of Rh6G followed Pseudo second order model and the equilibrium data was found to fit Freundlich model.
24950308	0	24	theme	Rhodamine	23:31	arg1	6G					33:34	Rhodamine 6G	23:34	Rhodamine 6G onto chitosan-g-(N-vinyl pyrrolidone)	23:72	Adsorption property of Rhodamine 6G onto chitosan-g-(N-vinyl pyrrolidone)/montmorillonite composite.
24950308	1	25	dep	in	202:203	arg1	situ					205:208	situ	205:208	situ	205:208	A series of chitosan-g-(N-vinyl pyrrolidone)/montmorillonite hydrogel composites were synthesized by in situ intercalative polymerization.
24950308	2	26	theme	scanning	305:312	arg1	microscopy					323:332	scanning electron microscopy	305:332	scanning electron microscopy	305:332	The properties of the composites were investigated by FTIR, XRD, scanning electron microscopy and differential scanning calorimetry.
24950308	1	27	theme	in	202:203	arg1	polymerization					224:237	in situ intercalative polymerization	202:237	in situ intercalative polymerization	202:237	A series of chitosan-g-(N-vinyl pyrrolidone)/montmorillonite hydrogel composites were synthesized by in situ intercalative polymerization.
24950308	3	28	theme	hydrogel	397:404	arg1	composites					406:415	the hydrogel composites	393:415	the hydrogel composites	393:415	The morphologies of the hydrogel composites were sponge like with a homogeneous structure.
24950308	5	29	theme	electrolyte	674:684	arg1	pH					649:650	the pH	645:650	the pH of the medium and the electrolyte	645:684	The swelling of the hydrogel composites formed was found to be affected by the pH of the medium and the electrolyte.
24950308	6	30	theme	water	823:827	arg1	dye					846:848	a water soluble cationic dye Rhodamine 6G (Rh6G)	821:868	a water soluble cationic dye Rhodamine 6G (Rh6G)	821:868	Maximum swelling was observed in the hydrogel containing low percentage of clay (H-0.2) and was used to investigate the adsorption of a water soluble cationic dye Rhodamine 6G (Rh6G).
24950308	7	31	from	increase	944:951	arg1	dose					956:959	dose	956:959	dose	956:959	The results show that the adsorption capacity for the dye increased with increase in dose, contact time but there was no change observed with respect to increase in temperature.
24950308	2	32	theme	scanning	351:358	arg1	calorimetry					360:370	differential scanning calorimetry	338:370	differential scanning calorimetry	338:370	The properties of the composites were investigated by FTIR, XRD, scanning electron microscopy and differential scanning calorimetry.
24950308	1	33	theme	intercalative	210:222	arg1	polymerization					224:237	in situ intercalative polymerization	202:237	in situ intercalative polymerization	202:237	A series of chitosan-g-(N-vinyl pyrrolidone)/montmorillonite hydrogel composites were synthesized by in situ intercalative polymerization.
24950308	0	34	theme	6G	33:34	arg1	property					11:18	Adsorption property	0:18	Adsorption property of Rhodamine 6G onto chitosan-g-(N-vinyl pyrrolidone)	0:72	Adsorption property of Rhodamine 6G onto chitosan-g-(N-vinyl pyrrolidone)/montmorillonite composite.
24950308	7	35	from	increase	1024:1031	arg1	temperature					1036:1046	temperature	1036:1046	temperature	1036:1046	The results show that the adsorption capacity for the dye increased with increase in dose, contact time but there was no change observed with respect to increase in temperature.
24950308	4	36	from	effects	468:474	arg1	structure					546:554	water structure	540:554	water structure	540:554	The effects of clay on the swelling, morphology, glass transition (Tg), and water structure were studied.
24950308	4	36	from	effects	468:474	arg1	Tg					531:532	Tg	531:532	Tg	531:532	The effects of clay on the swelling, morphology, glass transition (Tg), and water structure were studied.
24950308	4	36	from	effects	468:474	arg1	morphology					501:510	morphology	501:510	morphology	501:510	The effects of clay on the swelling, morphology, glass transition (Tg), and water structure were studied.
24950308	4	36	from	effects	468:474	arg1	transition					519:528	glass transition	513:528	glass transition (Tg)	513:533	The effects of clay on the swelling, morphology, glass transition (Tg), and water structure were studied.
24950308	4	36	from	effects	468:474	arg1	swelling					491:498	swelling	491:498	swelling	491:498	The effects of clay on the swelling, morphology, glass transition (Tg), and water structure were studied.
24950308	6	37	theme	low	744:746	arg1	percentage					748:757	the hydrogel containing low percentage	720:757	the hydrogel containing low percentage of clay (H-0.2)	720:773	Maximum swelling was observed in the hydrogel containing low percentage of clay (H-0.2) and was used to investigate the adsorption of a water soluble cationic dye Rhodamine 6G (Rh6G).
24950308	8	38	theme	equilibrium	1124:1134	arg1	data					1136:1139	the equilibrium data	1120:1139	the equilibrium data	1120:1139	The adsorption kinetics of Rh6G followed Pseudo second order model and the equilibrium data was found to fit Freundlich model.
24950308	4	39	theme	water	540:544	arg1	structure					546:554	water structure	540:554	water structure	540:554	The effects of clay on the swelling, morphology, glass transition (Tg), and water structure were studied.
24950308	6	40	theme	containing	733:742	arg1	percentage					748:757	the hydrogel containing low percentage	720:757	the hydrogel containing low percentage of clay (H-0.2)	720:773	Maximum swelling was observed in the hydrogel containing low percentage of clay (H-0.2) and was used to investigate the adsorption of a water soluble cationic dye Rhodamine 6G (Rh6G).
24950308	6	41	theme	hydrogel	724:731	arg1	percentage					748:757	the hydrogel containing low percentage	720:757	the hydrogel containing low percentage of clay (H-0.2)	720:773	Maximum swelling was observed in the hydrogel containing low percentage of clay (H-0.2) and was used to investigate the adsorption of a water soluble cationic dye Rhodamine 6G (Rh6G).
24950308	1	42	theme	chitosan-g-	113:123	arg1	composites					171:180	chitosan-g-(N-vinyl pyrrolidone)/montmorillonite hydrogel composites	113:180	chitosan-g-(N-vinyl pyrrolidone)/montmorillonite hydrogel composites	113:180	A series of chitosan-g-(N-vinyl pyrrolidone)/montmorillonite hydrogel composites were synthesized by in situ intercalative polymerization.
24950308	5	43	theme	medium	659:664	arg1	pH					649:650	the pH	645:650	the pH of the medium and the electrolyte	645:684	The swelling of the hydrogel composites formed was found to be affected by the pH of the medium and the electrolyte.
24950308	2	44	theme	differential	338:349	arg1	calorimetry					360:370	differential scanning calorimetry	338:370	differential scanning calorimetry	338:370	The properties of the composites were investigated by FTIR, XRD, scanning electron microscopy and differential scanning calorimetry.
24950308	7	45	theme	contact	962:968	arg1	time					970:973	contact time	962:973	contact time	962:973	The results show that the adsorption capacity for the dye increased with increase in dose, contact time but there was no change observed with respect to increase in temperature.
24950308	1	46	theme	N-vinyl	125:131	arg1	composites					171:180	chitosan-g-(N-vinyl pyrrolidone)/montmorillonite hydrogel composites	113:180	chitosan-g-(N-vinyl pyrrolidone)/montmorillonite hydrogel composites	113:180	A series of chitosan-g-(N-vinyl pyrrolidone)/montmorillonite hydrogel composites were synthesized by in situ intercalative polymerization.
24950308	8	47	theme	adsorption	1053:1062	arg1	kinetics					1064:1071	The adsorption kinetics	1049:1071	The adsorption kinetics of Rh6G	1049:1079	The adsorption kinetics of Rh6G followed Pseudo second order model and the equilibrium data was found to fit Freundlich model.
24950308	2	48	theme	composites	262:271	arg1	properties					244:253	The properties	240:253	The properties of the composites	240:271	The properties of the composites were investigated by FTIR, XRD, scanning electron microscopy and differential scanning calorimetry.
24950308	1	49	theme	pyrrolidone	133:143	arg1	composites					171:180	chitosan-g-(N-vinyl pyrrolidone)/montmorillonite hydrogel composites	113:180	chitosan-g-(N-vinyl pyrrolidone)/montmorillonite hydrogel composites	113:180	A series of chitosan-g-(N-vinyl pyrrolidone)/montmorillonite hydrogel composites were synthesized by in situ intercalative polymerization.
24950308	0	50	theme	N-vinyl	53:59	arg1	chitosan-g-					41:51	chitosan-g-	41:51	chitosan-g-(N-vinyl pyrrolidone)	41:72	Adsorption property of Rhodamine 6G onto chitosan-g-(N-vinyl pyrrolidone)/montmorillonite composite.
24950308	0	50	theme	N-vinyl	53:59	arg1	pyrrolidone					61:71	N-vinyl pyrrolidone	53:71	N-vinyl pyrrolidone	53:71	Adsorption property of Rhodamine 6G onto chitosan-g-(N-vinyl pyrrolidone)/montmorillonite composite.
24950308	4	51	theme	glass	513:517	arg1	Tg					531:532	Tg	531:532	Tg	531:532	The effects of clay on the swelling, morphology, glass transition (Tg), and water structure were studied.
24950308	4	51	theme	glass	513:517	arg1	transition					519:528	glass transition	513:528	glass transition (Tg)	513:533	The effects of clay on the swelling, morphology, glass transition (Tg), and water structure were studied.
24950308	6	52	located	observed	708:715	arg2	swelling					695:702	Maximum swelling	687:702	Maximum swelling	687:702	Maximum swelling was observed in the hydrogel containing low percentage of clay (H-0.2) and was used to investigate the adsorption of a water soluble cationic dye Rhodamine 6G (Rh6G).
24950308	6	52	located	observed	708:715	arg1	percentage					748:757	the hydrogel containing low percentage	720:757	the hydrogel containing low percentage of clay (H-0.2)	720:773	Maximum swelling was observed in the hydrogel containing low percentage of clay (H-0.2) and was used to investigate the adsorption of a water soluble cationic dye Rhodamine 6G (Rh6G).
24950308	6	53	dep	dye	846:848	arg1	Rh6G					864:867	Rh6G	864:867	Rh6G	864:867	Maximum swelling was observed in the hydrogel containing low percentage of clay (H-0.2) and was used to investigate the adsorption of a water soluble cationic dye Rhodamine 6G (Rh6G).
24950308	6	53	dep	dye	846:848	arg1	6G					860:861	Rhodamine 6G	850:861	a water soluble cationic dye Rhodamine 6G (Rh6G)	821:868	Maximum swelling was observed in the hydrogel containing low percentage of clay (H-0.2) and was used to investigate the adsorption of a water soluble cationic dye Rhodamine 6G (Rh6G).
25439875	10	0	theme	viscosity	1042:1050	arg1	properties					1052:1061	the best viscosity properties	1033:1061	the best viscosity properties	1033:1061	Pectins from raw peppers showed the best viscosity properties.
25439875	5	1	theme	*	652:652	arg1	values					654:659	the lowest L* values	640:659	the lowest L* values	640:659	Pectins from grilled peppers showed the lowest L* values.
25439875	2	2	theme	methyl	338:343	arg1	esterification					345:358	methyl esterification	338:358	methyl esterification	338:358	The yield, tristimulus color, degree of methyl esterification, monosaccharide composition, molecular weights distribution, and protein content depended on ripening and heat processing.
25439875	4	3	theme	abundant	587:594	arg1	pectin					596:601	the most abundant pectin	578:601	the most abundant pectin	578:601	The water-soluble pectin was the most abundant pectin.
25439875	4	3	theme	abundant	587:594	arg1	pectin					567:572	The water-soluble pectin	549:572	The water-soluble pectin	549:572	The water-soluble pectin was the most abundant pectin.
25439875	0	4	theme	rheological	66:76	arg1	properties					78:87	the physicochemical and rheological properties	42:87	the physicochemical and rheological properties of pepper pectins	42:105	Effect of ripening and heat processing on the physicochemical and rheological properties of pepper pectins.
25439875	10	5	theme	best	1037:1040	arg1	properties					1052:1061	the best viscosity properties	1033:1061	the best viscosity properties	1033:1061	Pectins from raw peppers showed the best viscosity properties.
25439875	3	6	theme	pectins	511:517	arg1	properties					497:506	The viscosity properties	483:506	The viscosity properties of pectins	483:517	The viscosity properties of pectins were independent of ripening.
25439875	3	6	theme	pectins	511:517	arg1	independent					524:534	independent	524:534	independent	524:534	The viscosity properties of pectins were independent of ripening.
25439875	7	7	theme	xylose	739:744	arg1	content					728:734	The content	724:734	The content of xylose, rhamnose, and mannose in pectins	724:778	The content of xylose, rhamnose, and mannose in pectins was highly altered by tested factors.
25439875	0	8	theme	pepper	92:97	arg1	pectins					99:105	pepper pectins	92:105	pepper pectins	92:105	Effect of ripening and heat processing on the physicochemical and rheological properties of pepper pectins.
25439875	0	9	from	Effect	0:5	arg1	properties					78:87	the physicochemical and rheological properties	42:87	the physicochemical and rheological properties of pepper pectins	42:105	Effect of ripening and heat processing on the physicochemical and rheological properties of pepper pectins.
25439875	8	10	dep	91.6	885:888	arg1	to					882:883	to	882:883	to	882:883	The degree of methyl esterification of pectins ranged from 26.8 to 91.6%.
25439875	8	11	theme	esterification	839:852	arg1	degree					822:827	The degree	818:827	The degree of methyl esterification of pectins	818:863	The degree of methyl esterification of pectins ranged from 26.8 to 91.6%.
25439875	9	12	theme	main	911:914	arg1	fraction					916:923	the main fraction	907:923	the main fraction of tested pectins	907:941	The peak Mw of the main fraction of tested pectins was sequentially reduced by ripening and heat processing.
25439875	5	13	theme	grilled	617:623	arg1	peppers					625:631	grilled peppers	617:631	grilled peppers	617:631	Pectins from grilled peppers showed the lowest L* values.
25439875	9	14	theme	fraction	916:923	arg1	Mw					901:902	The peak Mw	892:902	The peak Mw of the main fraction of tested pectins	892:941	The peak Mw of the main fraction of tested pectins was sequentially reduced by ripening and heat processing.
25439875	3	15	theme	viscosity	487:495	arg1	properties					497:506	The viscosity properties	483:506	The viscosity properties of pectins	483:517	The viscosity properties of pectins were independent of ripening.
25439875	3	15	theme	viscosity	487:495	arg1	independent					524:534	independent	524:534	independent	524:534	The viscosity properties of pectins were independent of ripening.
25439875	8	16	theme	pectins	857:863	arg1	esterification					839:852	methyl esterification	832:852	methyl esterification of pectins	832:863	The degree of methyl esterification of pectins ranged from 26.8 to 91.6%.
25439875	2	17	theme	molecular	389:397	arg1	weights					399:405	molecular weights	389:405	molecular weights distribution	389:418	The yield, tristimulus color, degree of methyl esterification, monosaccharide composition, molecular weights distribution, and protein content depended on ripening and heat processing.
25439875	0	18	theme	pectins	99:105	arg1	properties					78:87	the physicochemical and rheological properties	42:87	the physicochemical and rheological properties of pepper pectins	42:105	Effect of ripening and heat processing on the physicochemical and rheological properties of pepper pectins.
25439875	3	19	theme	ripening	539:546	arg1	properties					497:506	The viscosity properties	483:506	The viscosity properties of pectins	483:517	The viscosity properties of pectins were independent of ripening.
25439875	3	19	theme	ripening	539:546	arg1	independent					524:534	independent	524:534	independent	524:534	The viscosity properties of pectins were independent of ripening.
25439875	6	20	theme	protein	707:713	arg1	content					715:721	the highest protein content	695:721	the highest protein content	695:721	The alkali-soluble pectin showed the highest protein content.
25439875	9	21	theme	tested	928:933	arg1	pectins					935:941	tested pectins	928:941	tested pectins	928:941	The peak Mw of the main fraction of tested pectins was sequentially reduced by ripening and heat processing.
25439875	5	22	theme	lowest	644:649	arg1	values					654:659	the lowest L* values	640:659	the lowest L* values	640:659	Pectins from grilled peppers showed the lowest L* values.
25439875	6	23	theme	highest	699:705	arg1	content					715:721	the highest protein content	695:721	the highest protein content	695:721	The alkali-soluble pectin showed the highest protein content.
25439875	9	24	theme	pectins	935:941	arg1	fraction					916:923	the main fraction	907:923	the main fraction of tested pectins	907:941	The peak Mw of the main fraction of tested pectins was sequentially reduced by ripening and heat processing.
25439875	1	25	theme	rheological	258:268	arg1	properties					270:279	their physiochemical and rheological properties	233:279	properties	270:279	Water-, chelator-, and alkali-soluble pectins were isolated from raw and heat-processed Jalapeño peppers (green and red) and their physiochemical and rheological properties were determined.
25439875	5	26	theme	L	651:651	arg1	values					654:659	the lowest L* values	640:659	the lowest L* values	640:659	Pectins from grilled peppers showed the lowest L* values.
25439875	10	27	from	peppers	1018:1024	arg1	Pectins					1001:1007	Pectins	1001:1007	Pectins from raw peppers	1001:1024	Pectins from raw peppers showed the best viscosity properties.
25439875	2	28	theme	monosaccharide	361:374	arg1	composition					376:386	monosaccharide composition	361:386	monosaccharide composition	361:386	The yield, tristimulus color, degree of methyl esterification, monosaccharide composition, molecular weights distribution, and protein content depended on ripening and heat processing.
25439875	4	29	theme	water-soluble	553:565	arg1	pectin					596:601	the most abundant pectin	578:601	the most abundant pectin	578:601	The water-soluble pectin was the most abundant pectin.
25439875	4	29	theme	water-soluble	553:565	arg1	pectin					567:572	The water-soluble pectin	549:572	The water-soluble pectin	549:572	The water-soluble pectin was the most abundant pectin.
25439875	1	30	theme	heat-processed	181:194	arg1	peppers					205:211	heat-processed Jalapeño peppers	181:211	heat-processed Jalapeño peppers (green and red)	181:227	Water-, chelator-, and alkali-soluble pectins were isolated from raw and heat-processed Jalapeño peppers (green and red) and their physiochemical and rheological properties were determined.
25439875	2	31	theme	protein	425:431	arg1	content					433:439	protein content	425:439	protein content	425:439	The yield, tristimulus color, degree of methyl esterification, monosaccharide composition, molecular weights distribution, and protein content depended on ripening and heat processing.
25439875	7	32	from	content	728:734	arg1	pectins					772:778	pectins	772:778	pectins	772:778	The content of xylose, rhamnose, and mannose in pectins was highly altered by tested factors.
25439875	9	33	theme	peak	896:899	arg1	Mw					901:902	The peak Mw	892:902	The peak Mw of the main fraction of tested pectins	892:941	The peak Mw of the main fraction of tested pectins was sequentially reduced by ripening and heat processing.
25439875	1	34	theme	Jalapeño	196:203	arg1	peppers					205:211	heat-processed Jalapeño peppers	181:211	heat-processed Jalapeño peppers (green and red)	181:227	Water-, chelator-, and alkali-soluble pectins were isolated from raw and heat-processed Jalapeño peppers (green and red) and their physiochemical and rheological properties were determined.
25439875	0	35	theme	processing	28:37	arg1	Effect					0:5	Effect	0:5	Effect of ripening and heat processing on the physicochemical and rheological properties of pepper pectins.	0:106	Effect of ripening and heat processing on the physicochemical and rheological properties of pepper pectins.
25439875	1	36	attach	isolated	159:166	arg1	raw					173:175	raw	173:175	raw	173:175	Water-, chelator-, and alkali-soluble pectins were isolated from raw and heat-processed Jalapeño peppers (green and red) and their physiochemical and rheological properties were determined.
25439875	1	36	attach	isolated	159:166	arg2	pectins					146:152	Water-, chelator-, and alkali-soluble pectins	108:152	Water-, chelator-, and alkali-soluble pectins	108:152	Water-, chelator-, and alkali-soluble pectins were isolated from raw and heat-processed Jalapeño peppers (green and red) and their physiochemical and rheological properties were determined.
25439875	1	36	attach	isolated	159:166	arg1	peppers					205:211	heat-processed Jalapeño peppers	181:211	heat-processed Jalapeño peppers (green and red)	181:227	Water-, chelator-, and alkali-soluble pectins were isolated from raw and heat-processed Jalapeño peppers (green and red) and their physiochemical and rheological properties were determined.
25439875	6	37	theme	alkali-soluble	666:679	arg1	pectin					681:686	The alkali-soluble pectin	662:686	The alkali-soluble pectin	662:686	The alkali-soluble pectin showed the highest protein content.
25439875	2	38	theme	heat	466:469	arg1	processing					471:480	heat processing	466:480	heat processing	466:480	The yield, tristimulus color, degree of methyl esterification, monosaccharide composition, molecular weights distribution, and protein content depended on ripening and heat processing.
25439875	0	39	theme	heat	23:26	arg1	processing					28:37	heat processing	23:37	heat processing	23:37	Effect of ripening and heat processing on the physicochemical and rheological properties of pepper pectins.
25439875	2	40	theme	weights	399:405	arg1	distribution					407:418	molecular weights distribution	389:418	molecular weights distribution	389:418	The yield, tristimulus color, degree of methyl esterification, monosaccharide composition, molecular weights distribution, and protein content depended on ripening and heat processing.
25439875	5	41	from	peppers	625:631	arg1	Pectins					604:610	Pectins	604:610	Pectins from grilled peppers	604:631	Pectins from grilled peppers showed the lowest L* values.
25439875	7	42	theme	tested	802:807	arg1	factors					809:815	tested factors	802:815	tested factors	802:815	The content of xylose, rhamnose, and mannose in pectins was highly altered by tested factors.
25439875	1	43	theme	Water-	108:113	arg1	pectins					146:152	Water-, chelator-, and alkali-soluble pectins	108:152	Water-, chelator-, and alkali-soluble pectins	108:152	Water-, chelator-, and alkali-soluble pectins were isolated from raw and heat-processed Jalapeño peppers (green and red) and their physiochemical and rheological properties were determined.
25439875	2	44	theme	esterification	345:358	arg1	yield					302:306	The yield	298:306	The yield	298:306	The yield, tristimulus color, degree of methyl esterification, monosaccharide composition, molecular weights distribution, and protein content depended on ripening and heat processing.
25439875	2	44	theme	esterification	345:358	arg1	content					433:439	protein content	425:439	protein content	425:439	The yield, tristimulus color, degree of methyl esterification, monosaccharide composition, molecular weights distribution, and protein content depended on ripening and heat processing.
25439875	2	44	theme	esterification	345:358	arg1	distribution					407:418	molecular weights distribution	389:418	molecular weights distribution	389:418	The yield, tristimulus color, degree of methyl esterification, monosaccharide composition, molecular weights distribution, and protein content depended on ripening and heat processing.
25439875	2	44	theme	esterification	345:358	arg1	color					321:325	tristimulus color	309:325	tristimulus color	309:325	The yield, tristimulus color, degree of methyl esterification, monosaccharide composition, molecular weights distribution, and protein content depended on ripening and heat processing.
25439875	2	44	theme	esterification	345:358	arg1	degree					328:333	degree	328:333	degree of methyl esterification	328:358	The yield, tristimulus color, degree of methyl esterification, monosaccharide composition, molecular weights distribution, and protein content depended on ripening and heat processing.
25439875	2	44	theme	esterification	345:358	arg1	composition					376:386	monosaccharide composition	361:386	monosaccharide composition	361:386	The yield, tristimulus color, degree of methyl esterification, monosaccharide composition, molecular weights distribution, and protein content depended on ripening and heat processing.
25439875	7	45	theme	mannose	761:767	arg1	content					728:734	The content	724:734	The content of xylose, rhamnose, and mannose in pectins	724:778	The content of xylose, rhamnose, and mannose in pectins was highly altered by tested factors.
25439875	9	46	theme	heat	984:987	arg1	processing					989:998	heat processing	984:998	heat processing	984:998	The peak Mw of the main fraction of tested pectins was sequentially reduced by ripening and heat processing.
25439875	1	47	dep	peppers	205:211	arg1	red					224:226	red	224:226	red	224:226	Water-, chelator-, and alkali-soluble pectins were isolated from raw and heat-processed Jalapeño peppers (green and red) and their physiochemical and rheological properties were determined.
25439875	1	47	dep	peppers	205:211	arg1	green					214:218	green	214:218	green	214:218	Water-, chelator-, and alkali-soluble pectins were isolated from raw and heat-processed Jalapeño peppers (green and red) and their physiochemical and rheological properties were determined.
25439875	1	48	theme	chelator-	116:124	arg1	pectins					146:152	Water-, chelator-, and alkali-soluble pectins	108:152	Water-, chelator-, and alkali-soluble pectins	108:152	Water-, chelator-, and alkali-soluble pectins were isolated from raw and heat-processed Jalapeño peppers (green and red) and their physiochemical and rheological properties were determined.
25439875	10	49	theme	raw	1014:1016	arg1	peppers					1018:1024	raw peppers	1014:1024	raw peppers	1014:1024	Pectins from raw peppers showed the best viscosity properties.
25439875	2	50	theme	tristimulus	309:319	arg1	color					321:325	tristimulus color	309:325	tristimulus color	309:325	The yield, tristimulus color, degree of methyl esterification, monosaccharide composition, molecular weights distribution, and protein content depended on ripening and heat processing.
25439875	0	51	theme	physicochemical	46:60	arg1	properties					78:87	the physicochemical and rheological properties	42:87	the physicochemical and rheological properties of pepper pectins	42:105	Effect of ripening and heat processing on the physicochemical and rheological properties of pepper pectins.
25439875	0	52	theme	ripening	10:17	arg1	Effect					0:5	Effect	0:5	Effect of ripening and heat processing on the physicochemical and rheological properties of pepper pectins.	0:106	Effect of ripening and heat processing on the physicochemical and rheological properties of pepper pectins.
25439875	1	53	theme	alkali-soluble	131:144	arg1	pectins					146:152	Water-, chelator-, and alkali-soluble pectins	108:152	Water-, chelator-, and alkali-soluble pectins	108:152	Water-, chelator-, and alkali-soluble pectins were isolated from raw and heat-processed Jalapeño peppers (green and red) and their physiochemical and rheological properties were determined.
25439875	8	54	theme	methyl	832:837	arg1	esterification					839:852	methyl esterification	832:852	methyl esterification of pectins	832:863	The degree of methyl esterification of pectins ranged from 26.8 to 91.6%.
25439875	7	55	theme	rhamnose	747:754	arg1	content					728:734	The content	724:734	The content of xylose, rhamnose, and mannose in pectins	724:778	The content of xylose, rhamnose, and mannose in pectins was highly altered by tested factors.
25677379	1	0	gly	glycoproteins	263:275	arg1	glycoproteins					263:275	glycoproteins	263:275	glycoproteins	263:275	The cell wall of fungi is generally composed of an inner skeletal layer consisting of various polysaccharides surrounded by a layer of glycoproteins.
25677379	5	1	theme	Ca	777:778	arg1	-ATPase					790:796	a Ca(2+)/Mn(2+)-ATPase	775:796	a Ca(2+)/Mn(2+)-ATPase that plays an important role in the protein glycosylation pathway	775:862	We hypothesized that bcpmr1 also encodes a Ca(2+)/Mn(2+)-ATPase that plays an important role in the protein glycosylation pathway.
25677379	5	1	theme	Ca	777:778	arg1	bcpmr1					755:760	bcpmr1	755:760	bcpmr1	755:760	We hypothesized that bcpmr1 also encodes a Ca(2+)/Mn(2+)-ATPase that plays an important role in the protein glycosylation pathway.
25677379	9	2	dep	tomato	1375:1380	arg1	fruits					1393:1398	fruits	1393:1398	fruits	1393:1398	Importantly, the Δbcpmr1 mutant showed reduced virulence in tomato (leafs and fruits) and apple (fruits) and reduced biofilm formation.
25677379	9	2	dep	tomato	1375:1380	arg1	leafs					1383:1387	leafs	1383:1387	leafs	1383:1387	Importantly, the Δbcpmr1 mutant showed reduced virulence in tomato (leafs and fruits) and apple (fruits) and reduced biofilm formation.
25677379	0	3	theme	2+	34:35	arg1	bcpmr1					0:5	bcpmr1	0:5	bcpmr1	0:5	bcpmr1 encodes a P-type Ca(2+)/Mn(2+)-ATPase mediating cell-wall integrity and virulence in the phytopathogen Botrytis cinerea.
25677379	0	3	theme	2+	34:35	arg1	-ATPase					37:43	a P-type Ca(2+)/Mn(2+)-ATPase	15:43	a P-type Ca(2+)/Mn(2+)-ATPase mediating cell-wall integrity and virulence in the phytopathogen Botrytis cinerea	15:125	bcpmr1 encodes a P-type Ca(2+)/Mn(2+)-ATPase mediating cell-wall integrity and virulence in the phytopathogen Botrytis cinerea.
25677379	2	4	contain	contain	292:298	arg2	oligosaccharides					321:336	O-linked oligosaccharides	312:336	O-linked oligosaccharides	312:336	These usually contain both N- and O-linked oligosaccharides, coupled to the proteins by stepwise addition of mannose residues by mannosyltransferases in the endoplasmic reticulum and the Golgi apparatus.
25677379	2	4	contain	contain	292:298	arg1	These					278:282	These	278:282	These	278:282	These usually contain both N- and O-linked oligosaccharides, coupled to the proteins by stepwise addition of mannose residues by mannosyltransferases in the endoplasmic reticulum and the Golgi apparatus.
25677379	2	4	contain	contain	292:298	arg2	N-					305:306	N-	305:306	N-	305:306	These usually contain both N- and O-linked oligosaccharides, coupled to the proteins by stepwise addition of mannose residues by mannosyltransferases in the endoplasmic reticulum and the Golgi apparatus.
25677379	10	5	theme	bcpmr1	1501:1506	arg1	importance					1487:1496	the importance	1483:1496	the importance of bcpmr1 for protein glycosylation, cell wall structure and virulence of B. cinerea	1483:1581	Together, our results highlight the importance of bcpmr1 for protein glycosylation, cell wall structure and virulence of B. cinerea.
25677379	9	6	from	formation	1440:1448	arg1	apple					1405:1409	apple	1405:1409	apple	1405:1409	Importantly, the Δbcpmr1 mutant showed reduced virulence in tomato (leafs and fruits) and apple (fruits) and reduced biofilm formation.
25677379	9	6	from	formation	1440:1448	arg1	tomato					1375:1380	tomato	1375:1380	tomato	1375:1380	Importantly, the Δbcpmr1 mutant showed reduced virulence in tomato (leafs and fruits) and apple (fruits) and reduced biofilm formation.
25677379	9	6	from	formation	1440:1448	arg1	fruits					1412:1417	fruits	1412:1417	fruits	1412:1417	Importantly, the Δbcpmr1 mutant showed reduced virulence in tomato (leafs and fruits) and apple (fruits) and reduced biofilm formation.
25677379	9	7	theme	Δbcpmr1	1332:1338	arg1	mutant					1340:1345	the Δbcpmr1 mutant	1328:1345	the Δbcpmr1 mutant	1328:1345	Importantly, the Δbcpmr1 mutant showed reduced virulence in tomato (leafs and fruits) and apple (fruits) and reduced biofilm formation.
25677379	7	8	theme	Significant	1016:1026	arg1	alterations					1028:1038	Significant alterations	1016:1038	Significant alterations in hyphal cell wall composition	1016:1070	Significant alterations in hyphal cell wall composition were observed including a 83% decrease of mannan levels and an increase in the amount of chitin and glucan.
25677379	7	9	theme	glucan	1172:1177	arg1	amount					1151:1156	the amount	1147:1156	the amount of chitin and glucan	1147:1177	Significant alterations in hyphal cell wall composition were observed including a 83% decrease of mannan levels and an increase in the amount of chitin and glucan.
25677379	7	9	theme	glucan	1172:1177	arg1	glucan					1172:1177	glucan	1172:1177	glucan	1172:1177	Significant alterations in hyphal cell wall composition were observed including a 83% decrease of mannan levels and an increase in the amount of chitin and glucan.
25677379	7	9	theme	glucan	1172:1177	arg1	chitin					1161:1166	chitin	1161:1166	chitin	1161:1166	Significant alterations in hyphal cell wall composition were observed including a 83% decrease of mannan levels and an increase in the amount of chitin and glucan.
25677379	10	10	gly	glycosylation	1520:1532	arg1	cinerea					1575:1581	B. cinerea	1572:1581	B. cinerea	1572:1581	Together, our results highlight the importance of bcpmr1 for protein glycosylation, cell wall structure and virulence of B. cinerea.
25677379	7	11	from	decrease	1102:1109	arg1	amount					1151:1156	the amount	1147:1156	the amount of chitin and glucan	1147:1177	Significant alterations in hyphal cell wall composition were observed including a 83% decrease of mannan levels and an increase in the amount of chitin and glucan.
25677379	7	11	from	decrease	1102:1109	arg1	glucan					1172:1177	glucan	1172:1177	glucan	1172:1177	Significant alterations in hyphal cell wall composition were observed including a 83% decrease of mannan levels and an increase in the amount of chitin and glucan.
25677379	7	11	from	decrease	1102:1109	arg1	chitin					1161:1166	chitin	1161:1166	chitin	1161:1166	Significant alterations in hyphal cell wall composition were observed including a 83% decrease of mannan levels and an increase in the amount of chitin and glucan.
25677379	3	12	theme	essential	495:503	arg1	Mn					556:557	Mn	556:557	Mn(2+) provided by the Ca(2+)/Mn(2+)-ATPase known as Pmr1	556:612	In yeast, an essential luminal cofactor for these mannosyltransferases is Mn(2+) provided by the Ca(2+)/Mn(2+)-ATPase known as Pmr1.
25677379	3	12	theme	essential	495:503	arg1	cofactor					513:520	an essential luminal cofactor	492:520	an essential luminal cofactor for these mannosyltransferases	492:551	In yeast, an essential luminal cofactor for these mannosyltransferases is Mn(2+) provided by the Ca(2+)/Mn(2+)-ATPase known as Pmr1.
25677379	7	13	theme	levels	1121:1126	arg1	decrease					1102:1109	a 83% decrease	1096:1109	a 83% decrease of mannan levels	1096:1126	Significant alterations in hyphal cell wall composition were observed including a 83% decrease of mannan levels and an increase in the amount of chitin and glucan.
25677379	7	13	theme	levels	1121:1126	arg1	increase					1135:1142	an increase	1132:1142	an increase in the amount of chitin and glucan	1132:1177	Significant alterations in hyphal cell wall composition were observed including a 83% decrease of mannan levels and an increase in the amount of chitin and glucan.
25677379	9	14	theme	reduced	1354:1360	arg1	virulence					1362:1370	reduced virulence	1354:1370	reduced virulence in tomato (leafs and fruits) and apple (fruits)	1354:1418	Importantly, the Δbcpmr1 mutant showed reduced virulence in tomato (leafs and fruits) and apple (fruits) and reduced biofilm formation.
25677379	10	15	theme	cinerea	1575:1581	arg1	structure					1545:1553	cell wall structure	1535:1553	cell wall structure	1535:1553	Together, our results highlight the importance of bcpmr1 for protein glycosylation, cell wall structure and virulence of B. cinerea.
25677379	10	15	theme	cinerea	1575:1581	arg1	glycosylation					1520:1532	protein glycosylation	1512:1532	protein glycosylation	1512:1532	Together, our results highlight the importance of bcpmr1 for protein glycosylation, cell wall structure and virulence of B. cinerea.
25677379	10	15	theme	cinerea	1575:1581	arg1	virulence					1559:1567	virulence	1559:1567	virulence	1559:1567	Together, our results highlight the importance of bcpmr1 for protein glycosylation, cell wall structure and virulence of B. cinerea.
25677379	9	16	from	virulence	1362:1370	arg1	apple					1405:1409	apple	1405:1409	apple	1405:1409	Importantly, the Δbcpmr1 mutant showed reduced virulence in tomato (leafs and fruits) and apple (fruits) and reduced biofilm formation.
25677379	9	16	from	virulence	1362:1370	arg1	tomato					1375:1380	tomato	1375:1380	tomato	1375:1380	Importantly, the Δbcpmr1 mutant showed reduced virulence in tomato (leafs and fruits) and apple (fruits) and reduced biofilm formation.
25677379	9	16	from	virulence	1362:1370	arg1	fruits					1412:1417	fruits	1412:1417	fruits	1412:1417	Importantly, the Δbcpmr1 mutant showed reduced virulence in tomato (leafs and fruits) and apple (fruits) and reduced biofilm formation.
25677379	3	17	theme	luminal	505:511	arg1	Mn					556:557	Mn	556:557	Mn(2+) provided by the Ca(2+)/Mn(2+)-ATPase known as Pmr1	556:612	In yeast, an essential luminal cofactor for these mannosyltransferases is Mn(2+) provided by the Ca(2+)/Mn(2+)-ATPase known as Pmr1.
25677379	3	17	theme	luminal	505:511	arg1	cofactor					513:520	an essential luminal cofactor	492:520	an essential luminal cofactor for these mannosyltransferases	492:551	In yeast, an essential luminal cofactor for these mannosyltransferases is Mn(2+) provided by the Ca(2+)/Mn(2+)-ATPase known as Pmr1.
25677379	10	18	theme	cell	1535:1538	arg1	structure					1545:1553	cell wall structure	1535:1553	cell wall structure	1535:1553	Together, our results highlight the importance of bcpmr1 for protein glycosylation, cell wall structure and virulence of B. cinerea.
25677379	1	19	theme	various	214:220	arg1	polysaccharides					222:236	various polysaccharides	214:236	various polysaccharides surrounded by a layer of glycoproteins	214:275	The cell wall of fungi is generally composed of an inner skeletal layer consisting of various polysaccharides surrounded by a layer of glycoproteins.
25677379	7	20	theme	cell	1050:1053	arg1	composition					1060:1070	hyphal cell wall composition	1043:1070	hyphal cell wall composition	1043:1070	Significant alterations in hyphal cell wall composition were observed including a 83% decrease of mannan levels and an increase in the amount of chitin and glucan.
25677379	2	21	from	mannosyltransferases	407:426	arg1	reticulum					447:455	the endoplasmic reticulum	431:455	the endoplasmic reticulum	431:455	These usually contain both N- and O-linked oligosaccharides, coupled to the proteins by stepwise addition of mannose residues by mannosyltransferases in the endoplasmic reticulum and the Golgi apparatus.
25677379	2	21	from	mannosyltransferases	407:426	arg1	apparatus					471:479	the Golgi apparatus	461:479	the Golgi apparatus	461:479	These usually contain both N- and O-linked oligosaccharides, coupled to the proteins by stepwise addition of mannose residues by mannosyltransferases in the endoplasmic reticulum and the Golgi apparatus.
25677379	4	22	theme	PMR1	728:731	arg1	gene					693:696	the Botrytis cinerea pmr1 gene	667:696	the Botrytis cinerea pmr1 gene	667:696	In this study, we have identified and characterized the Botrytis cinerea pmr1 gene, the closest homolog of yeast PMR1.
25677379	4	22	theme	PMR1	728:731	arg1	homolog					711:717	the closest homolog	699:717	the closest homolog of yeast PMR1	699:731	In this study, we have identified and characterized the Botrytis cinerea pmr1 gene, the closest homolog of yeast PMR1.
25677379	6	23	theme	bcpmr1	897:902	arg1	mutants					909:915	bcpmr1 null mutants	897:915	bcpmr1 null mutants	897:915	Phenotypic analysis showed that bcpmr1 null mutants displayed a significant reduction in conidial production, radial growth and diameter of sclerotia.
25677379	4	24	theme	Botrytis	671:678	arg1	gene					693:696	the Botrytis cinerea pmr1 gene	667:696	the Botrytis cinerea pmr1 gene	667:696	In this study, we have identified and characterized the Botrytis cinerea pmr1 gene, the closest homolog of yeast PMR1.
25677379	4	24	theme	Botrytis	671:678	arg1	homolog					711:717	the closest homolog	699:717	the closest homolog of yeast PMR1	699:731	In this study, we have identified and characterized the Botrytis cinerea pmr1 gene, the closest homolog of yeast PMR1.
25677379	6	25	theme	significant	929:939	arg1	reduction					941:949	a significant reduction	927:949	a significant reduction in conidial production, radial growth and diameter of sclerotia	927:1013	Phenotypic analysis showed that bcpmr1 null mutants displayed a significant reduction in conidial production, radial growth and diameter of sclerotia.
25677379	0	26	theme	cell-wall	55:63	arg1	integrity					65:73	cell-wall integrity	55:73	cell-wall integrity	55:73	bcpmr1 encodes a P-type Ca(2+)/Mn(2+)-ATPase mediating cell-wall integrity and virulence in the phytopathogen Botrytis cinerea.
25677379	4	27	theme	yeast	722:726	arg1	PMR1					728:731	yeast PMR1	722:731	yeast PMR1	722:731	In this study, we have identified and characterized the Botrytis cinerea pmr1 gene, the closest homolog of yeast PMR1.
25677379	6	28	theme	Phenotypic	865:874	arg1	analysis					876:883	Phenotypic analysis	865:883	Phenotypic analysis	865:883	Phenotypic analysis showed that bcpmr1 null mutants displayed a significant reduction in conidial production, radial growth and diameter of sclerotia.
25677379	8	29	theme	wall-perturbing	1241:1255	arg1	agents					1257:1262	cell wall-perturbing agents	1236:1262	cell wall-perturbing agents such as Calcofluor white, Congo red and zymolyase	1236:1312	These changes were accompanied by a hypersensitivity to cell wall-perturbing agents such as Calcofluor white, Congo red and zymolyase.
25677379	8	29	theme	wall-perturbing	1241:1255	arg1	zymolyase					1304:1312	zymolyase	1304:1312	zymolyase	1304:1312	These changes were accompanied by a hypersensitivity to cell wall-perturbing agents such as Calcofluor white, Congo red and zymolyase.
25677379	8	29	theme	wall-perturbing	1241:1255	arg1	white					1283:1287	white	1283:1287	white	1283:1287	These changes were accompanied by a hypersensitivity to cell wall-perturbing agents such as Calcofluor white, Congo red and zymolyase.
25677379	8	29	theme	wall-perturbing	1241:1255	arg1	red					1296:1298	red	1296:1298	red	1296:1298	These changes were accompanied by a hypersensitivity to cell wall-perturbing agents such as Calcofluor white, Congo red and zymolyase.
25677379	1	30	theme	cell	132:135	arg1	wall					137:140	The cell wall	128:140	The cell wall of fungi	128:149	The cell wall of fungi is generally composed of an inner skeletal layer consisting of various polysaccharides surrounded by a layer of glycoproteins.
25677379	2	31	link	O-linked	312:319	arg1	oligosaccharides					321:336	O-linked oligosaccharides	312:336	O-linked oligosaccharides	312:336	These usually contain both N- and O-linked oligosaccharides, coupled to the proteins by stepwise addition of mannose residues by mannosyltransferases in the endoplasmic reticulum and the Golgi apparatus.
25677379	5	32	theme	2+	787:788	arg1	-ATPase					790:796	a Ca(2+)/Mn(2+)-ATPase	775:796	a Ca(2+)/Mn(2+)-ATPase that plays an important role in the protein glycosylation pathway	775:862	We hypothesized that bcpmr1 also encodes a Ca(2+)/Mn(2+)-ATPase that plays an important role in the protein glycosylation pathway.
25677379	5	32	theme	2+	787:788	arg1	bcpmr1					755:760	bcpmr1	755:760	bcpmr1	755:760	We hypothesized that bcpmr1 also encodes a Ca(2+)/Mn(2+)-ATPase that plays an important role in the protein glycosylation pathway.
25677379	7	33	from	alterations	1028:1038	arg1	composition					1060:1070	hyphal cell wall composition	1043:1070	hyphal cell wall composition	1043:1070	Significant alterations in hyphal cell wall composition were observed including a 83% decrease of mannan levels and an increase in the amount of chitin and glucan.
25677379	7	34	theme	chitin	1161:1166	arg1	amount					1151:1156	the amount	1147:1156	the amount of chitin and glucan	1147:1177	Significant alterations in hyphal cell wall composition were observed including a 83% decrease of mannan levels and an increase in the amount of chitin and glucan.
25677379	7	34	theme	chitin	1161:1166	arg1	glucan					1172:1177	glucan	1172:1177	glucan	1172:1177	Significant alterations in hyphal cell wall composition were observed including a 83% decrease of mannan levels and an increase in the amount of chitin and glucan.
25677379	7	34	theme	chitin	1161:1166	arg1	chitin					1161:1166	chitin	1161:1166	chitin	1161:1166	Significant alterations in hyphal cell wall composition were observed including a 83% decrease of mannan levels and an increase in the amount of chitin and glucan.
25677379	2	35	theme	residues	395:402	arg1	addition					375:382	stepwise addition	366:382	stepwise addition of mannose residues	366:402	These usually contain both N- and O-linked oligosaccharides, coupled to the proteins by stepwise addition of mannose residues by mannosyltransferases in the endoplasmic reticulum and the Golgi apparatus.
25677379	10	36	theme	protein	1512:1518	arg1	glycosylation					1520:1532	protein glycosylation	1512:1532	protein glycosylation	1512:1532	Together, our results highlight the importance of bcpmr1 for protein glycosylation, cell wall structure and virulence of B. cinerea.
25677379	6	37	theme	sclerotia	1005:1013	arg1	diameter					993:1000	diameter	993:1000	diameter of sclerotia	993:1013	Phenotypic analysis showed that bcpmr1 null mutants displayed a significant reduction in conidial production, radial growth and diameter of sclerotia.
25677379	6	37	theme	sclerotia	1005:1013	arg1	production					963:972	conidial production	954:972	conidial production	954:972	Phenotypic analysis showed that bcpmr1 null mutants displayed a significant reduction in conidial production, radial growth and diameter of sclerotia.
25677379	6	37	theme	sclerotia	1005:1013	arg1	growth					982:987	radial growth	975:987	radial growth	975:987	Phenotypic analysis showed that bcpmr1 null mutants displayed a significant reduction in conidial production, radial growth and diameter of sclerotia.
25677379	0	38	theme	P-type	17:22	arg1	bcpmr1					0:5	bcpmr1	0:5	bcpmr1	0:5	bcpmr1 encodes a P-type Ca(2+)/Mn(2+)-ATPase mediating cell-wall integrity and virulence in the phytopathogen Botrytis cinerea.
25677379	0	38	theme	P-type	17:22	arg1	-ATPase					37:43	a P-type Ca(2+)/Mn(2+)-ATPase	15:43	a P-type Ca(2+)/Mn(2+)-ATPase mediating cell-wall integrity and virulence in the phytopathogen Botrytis cinerea	15:125	bcpmr1 encodes a P-type Ca(2+)/Mn(2+)-ATPase mediating cell-wall integrity and virulence in the phytopathogen Botrytis cinerea.
25677379	7	39	from	increase	1135:1142	arg1	amount					1151:1156	the amount	1147:1156	the amount of chitin and glucan	1147:1177	Significant alterations in hyphal cell wall composition were observed including a 83% decrease of mannan levels and an increase in the amount of chitin and glucan.
25677379	7	39	from	increase	1135:1142	arg1	glucan					1172:1177	glucan	1172:1177	glucan	1172:1177	Significant alterations in hyphal cell wall composition were observed including a 83% decrease of mannan levels and an increase in the amount of chitin and glucan.
25677379	7	39	from	increase	1135:1142	arg1	chitin					1161:1166	chitin	1161:1166	chitin	1161:1166	Significant alterations in hyphal cell wall composition were observed including a 83% decrease of mannan levels and an increase in the amount of chitin and glucan.
25677379	3	40	from	Mn	556:557	arg1	yeast					485:489	yeast	485:489	yeast	485:489	In yeast, an essential luminal cofactor for these mannosyltransferases is Mn(2+) provided by the Ca(2+)/Mn(2+)-ATPase known as Pmr1.
25677379	1	41	theme	fungi	145:149	arg1	wall					137:140	The cell wall	128:140	The cell wall of fungi	128:149	The cell wall of fungi is generally composed of an inner skeletal layer consisting of various polysaccharides surrounded by a layer of glycoproteins.
25677379	6	42	from	reduction	941:949	arg1	diameter					993:1000	diameter	993:1000	diameter of sclerotia	993:1013	Phenotypic analysis showed that bcpmr1 null mutants displayed a significant reduction in conidial production, radial growth and diameter of sclerotia.
25677379	6	42	from	reduction	941:949	arg1	production					963:972	conidial production	954:972	conidial production	954:972	Phenotypic analysis showed that bcpmr1 null mutants displayed a significant reduction in conidial production, radial growth and diameter of sclerotia.
25677379	6	42	from	reduction	941:949	arg1	growth					982:987	radial growth	975:987	radial growth	975:987	Phenotypic analysis showed that bcpmr1 null mutants displayed a significant reduction in conidial production, radial growth and diameter of sclerotia.
25677379	5	43	theme	important	812:820	arg1	role					822:825	an important role	809:825	an important role	809:825	We hypothesized that bcpmr1 also encodes a Ca(2+)/Mn(2+)-ATPase that plays an important role in the protein glycosylation pathway.
25677379	3	44	theme	2+	582:583	arg1	-ATPase					592:598	the Ca(2+)/Mn(2+)-ATPase	575:598	the Ca(2+)/Mn(2+)-ATPase known as Pmr1	575:612	In yeast, an essential luminal cofactor for these mannosyltransferases is Mn(2+) provided by the Ca(2+)/Mn(2+)-ATPase known as Pmr1.
25677379	10	45	theme	wall	1540:1543	arg1	structure					1545:1553	cell wall structure	1535:1553	cell wall structure	1535:1553	Together, our results highlight the importance of bcpmr1 for protein glycosylation, cell wall structure and virulence of B. cinerea.
25677379	2	46	theme	stepwise	366:373	arg1	addition					375:382	stepwise addition	366:382	stepwise addition of mannose residues	366:402	These usually contain both N- and O-linked oligosaccharides, coupled to the proteins by stepwise addition of mannose residues by mannosyltransferases in the endoplasmic reticulum and the Golgi apparatus.
25677379	2	47	theme	endoplasmic	435:445	arg1	reticulum					447:455	the endoplasmic reticulum	431:455	the endoplasmic reticulum	431:455	These usually contain both N- and O-linked oligosaccharides, coupled to the proteins by stepwise addition of mannose residues by mannosyltransferases in the endoplasmic reticulum and the Golgi apparatus.
25677379	7	48	theme	hyphal	1043:1048	arg1	composition					1060:1070	hyphal cell wall composition	1043:1070	hyphal cell wall composition	1043:1070	Significant alterations in hyphal cell wall composition were observed including a 83% decrease of mannan levels and an increase in the amount of chitin and glucan.
25677379	4	49	theme	closest	703:709	arg1	gene					693:696	the Botrytis cinerea pmr1 gene	667:696	the Botrytis cinerea pmr1 gene	667:696	In this study, we have identified and characterized the Botrytis cinerea pmr1 gene, the closest homolog of yeast PMR1.
25677379	4	49	theme	closest	703:709	arg1	homolog					711:717	the closest homolog	699:717	the closest homolog of yeast PMR1	699:731	In this study, we have identified and characterized the Botrytis cinerea pmr1 gene, the closest homolog of yeast PMR1.
25677379	0	50	theme	Ca	24:25	arg1	bcpmr1					0:5	bcpmr1	0:5	bcpmr1	0:5	bcpmr1 encodes a P-type Ca(2+)/Mn(2+)-ATPase mediating cell-wall integrity and virulence in the phytopathogen Botrytis cinerea.
25677379	0	50	theme	Ca	24:25	arg1	-ATPase					37:43	a P-type Ca(2+)/Mn(2+)-ATPase	15:43	a P-type Ca(2+)/Mn(2+)-ATPase mediating cell-wall integrity and virulence in the phytopathogen Botrytis cinerea	15:125	bcpmr1 encodes a P-type Ca(2+)/Mn(2+)-ATPase mediating cell-wall integrity and virulence in the phytopathogen Botrytis cinerea.
25677379	3	51	theme	Ca	579:580	arg1	-ATPase					592:598	the Ca(2+)/Mn(2+)-ATPase	575:598	the Ca(2+)/Mn(2+)-ATPase known as Pmr1	575:612	In yeast, an essential luminal cofactor for these mannosyltransferases is Mn(2+) provided by the Ca(2+)/Mn(2+)-ATPase known as Pmr1.
25677379	2	52	theme	mannose	387:393	arg1	residues					395:402	mannose residues	387:402	mannose residues	387:402	These usually contain both N- and O-linked oligosaccharides, coupled to the proteins by stepwise addition of mannose residues by mannosyltransferases in the endoplasmic reticulum and the Golgi apparatus.
25677379	7	53	theme	wall	1055:1058	arg1	composition					1060:1070	hyphal cell wall composition	1043:1070	hyphal cell wall composition	1043:1070	Significant alterations in hyphal cell wall composition were observed including a 83% decrease of mannan levels and an increase in the amount of chitin and glucan.
25677379	0	54	theme	Botrytis	110:117	arg1	cinerea					119:125	the phytopathogen Botrytis cinerea	92:125	the phytopathogen Botrytis cinerea	92:125	bcpmr1 encodes a P-type Ca(2+)/Mn(2+)-ATPase mediating cell-wall integrity and virulence in the phytopathogen Botrytis cinerea.
25677379	7	55	theme	83	1098:1099	arg1	%					1100:1100	%	1100:1100	%	1100:1100	Significant alterations in hyphal cell wall composition were observed including a 83% decrease of mannan levels and an increase in the amount of chitin and glucan.
25677379	9	56	theme	reduced	1424:1430	arg1	formation					1440:1448	reduced biofilm formation	1424:1448	reduced biofilm formation	1424:1448	Importantly, the Δbcpmr1 mutant showed reduced virulence in tomato (leafs and fruits) and apple (fruits) and reduced biofilm formation.
25677379	5	57	theme	2+	780:781	arg1	-ATPase					790:796	a Ca(2+)/Mn(2+)-ATPase	775:796	a Ca(2+)/Mn(2+)-ATPase that plays an important role in the protein glycosylation pathway	775:862	We hypothesized that bcpmr1 also encodes a Ca(2+)/Mn(2+)-ATPase that plays an important role in the protein glycosylation pathway.
25677379	5	57	theme	2+	780:781	arg1	bcpmr1					755:760	bcpmr1	755:760	bcpmr1	755:760	We hypothesized that bcpmr1 also encodes a Ca(2+)/Mn(2+)-ATPase that plays an important role in the protein glycosylation pathway.
25677379	0	58	theme	2+	27:28	arg1	bcpmr1					0:5	bcpmr1	0:5	bcpmr1	0:5	bcpmr1 encodes a P-type Ca(2+)/Mn(2+)-ATPase mediating cell-wall integrity and virulence in the phytopathogen Botrytis cinerea.
25677379	0	58	theme	2+	27:28	arg1	-ATPase					37:43	a P-type Ca(2+)/Mn(2+)-ATPase	15:43	a P-type Ca(2+)/Mn(2+)-ATPase mediating cell-wall integrity and virulence in the phytopathogen Botrytis cinerea	15:125	bcpmr1 encodes a P-type Ca(2+)/Mn(2+)-ATPase mediating cell-wall integrity and virulence in the phytopathogen Botrytis cinerea.
25677379	1	59	theme	glycoproteins	263:275	arg1	layer					254:258	a layer	252:258	a layer of glycoproteins	252:275	The cell wall of fungi is generally composed of an inner skeletal layer consisting of various polysaccharides surrounded by a layer of glycoproteins.
25677379	3	60	theme	2+	589:590	arg1	-ATPase					592:598	the Ca(2+)/Mn(2+)-ATPase	575:598	the Ca(2+)/Mn(2+)-ATPase known as Pmr1	575:612	In yeast, an essential luminal cofactor for these mannosyltransferases is Mn(2+) provided by the Ca(2+)/Mn(2+)-ATPase known as Pmr1.
25677379	9	61	theme	biofilm	1432:1438	arg1	formation					1440:1448	reduced biofilm formation	1424:1448	reduced biofilm formation	1424:1448	Importantly, the Δbcpmr1 mutant showed reduced virulence in tomato (leafs and fruits) and apple (fruits) and reduced biofilm formation.
25677379	7	62	theme	mannan	1114:1119	arg1	levels					1121:1126	mannan levels	1114:1126	mannan levels	1114:1126	Significant alterations in hyphal cell wall composition were observed including a 83% decrease of mannan levels and an increase in the amount of chitin and glucan.
25677379	4	63	theme	pmr1	688:691	arg1	gene					693:696	the Botrytis cinerea pmr1 gene	667:696	the Botrytis cinerea pmr1 gene	667:696	In this study, we have identified and characterized the Botrytis cinerea pmr1 gene, the closest homolog of yeast PMR1.
25677379	4	63	theme	pmr1	688:691	arg1	homolog					711:717	the closest homolog	699:717	the closest homolog of yeast PMR1	699:731	In this study, we have identified and characterized the Botrytis cinerea pmr1 gene, the closest homolog of yeast PMR1.
25677379	8	64	theme	cell	1236:1239	arg1	agents					1257:1262	cell wall-perturbing agents	1236:1262	cell wall-perturbing agents such as Calcofluor white, Congo red and zymolyase	1236:1312	These changes were accompanied by a hypersensitivity to cell wall-perturbing agents such as Calcofluor white, Congo red and zymolyase.
25677379	8	64	theme	cell	1236:1239	arg1	zymolyase					1304:1312	zymolyase	1304:1312	zymolyase	1304:1312	These changes were accompanied by a hypersensitivity to cell wall-perturbing agents such as Calcofluor white, Congo red and zymolyase.
25677379	8	64	theme	cell	1236:1239	arg1	white					1283:1287	white	1283:1287	white	1283:1287	These changes were accompanied by a hypersensitivity to cell wall-perturbing agents such as Calcofluor white, Congo red and zymolyase.
25677379	8	64	theme	cell	1236:1239	arg1	red					1296:1298	red	1296:1298	red	1296:1298	These changes were accompanied by a hypersensitivity to cell wall-perturbing agents such as Calcofluor white, Congo red and zymolyase.
25677379	5	65	theme	/Mn	783:785	arg1	-ATPase					790:796	a Ca(2+)/Mn(2+)-ATPase	775:796	a Ca(2+)/Mn(2+)-ATPase that plays an important role in the protein glycosylation pathway	775:862	We hypothesized that bcpmr1 also encodes a Ca(2+)/Mn(2+)-ATPase that plays an important role in the protein glycosylation pathway.
25677379	5	65	theme	/Mn	783:785	arg1	bcpmr1					755:760	bcpmr1	755:760	bcpmr1	755:760	We hypothesized that bcpmr1 also encodes a Ca(2+)/Mn(2+)-ATPase that plays an important role in the protein glycosylation pathway.
25677379	3	66	theme	/Mn	585:587	arg1	-ATPase					592:598	the Ca(2+)/Mn(2+)-ATPase	575:598	the Ca(2+)/Mn(2+)-ATPase known as Pmr1	575:612	In yeast, an essential luminal cofactor for these mannosyltransferases is Mn(2+) provided by the Ca(2+)/Mn(2+)-ATPase known as Pmr1.
25677379	4	67	theme	cinerea	680:686	arg1	gene					693:696	the Botrytis cinerea pmr1 gene	667:696	the Botrytis cinerea pmr1 gene	667:696	In this study, we have identified and characterized the Botrytis cinerea pmr1 gene, the closest homolog of yeast PMR1.
25677379	4	67	theme	cinerea	680:686	arg1	homolog					711:717	the closest homolog	699:717	the closest homolog of yeast PMR1	699:731	In this study, we have identified and characterized the Botrytis cinerea pmr1 gene, the closest homolog of yeast PMR1.
25677379	6	68	theme	conidial	954:961	arg1	production					963:972	conidial production	954:972	conidial production	954:972	Phenotypic analysis showed that bcpmr1 null mutants displayed a significant reduction in conidial production, radial growth and diameter of sclerotia.
25677379	6	69	theme	radial	975:980	arg1	growth					982:987	radial growth	975:987	radial growth	975:987	Phenotypic analysis showed that bcpmr1 null mutants displayed a significant reduction in conidial production, radial growth and diameter of sclerotia.
25677379	2	70	theme	Golgi	465:469	arg1	apparatus					471:479	the Golgi apparatus	461:479	the Golgi apparatus	461:479	These usually contain both N- and O-linked oligosaccharides, coupled to the proteins by stepwise addition of mannose residues by mannosyltransferases in the endoplasmic reticulum and the Golgi apparatus.
25677379	5	71	theme	protein	834:840	arg1	pathway					856:862	the protein glycosylation pathway	830:862	the protein glycosylation pathway	830:862	We hypothesized that bcpmr1 also encodes a Ca(2+)/Mn(2+)-ATPase that plays an important role in the protein glycosylation pathway.
25677379	6	72	theme	null	904:907	arg1	mutants					909:915	bcpmr1 null mutants	897:915	bcpmr1 null mutants	897:915	Phenotypic analysis showed that bcpmr1 null mutants displayed a significant reduction in conidial production, radial growth and diameter of sclerotia.
25677379	1	73	theme	inner	179:183	arg1	layer					194:198	an inner skeletal layer	176:198	an inner skeletal layer consisting of various polysaccharides surrounded by a layer of glycoproteins	176:275	The cell wall of fungi is generally composed of an inner skeletal layer consisting of various polysaccharides surrounded by a layer of glycoproteins.
25677379	0	74	theme	/Mn	30:32	arg1	bcpmr1					0:5	bcpmr1	0:5	bcpmr1	0:5	bcpmr1 encodes a P-type Ca(2+)/Mn(2+)-ATPase mediating cell-wall integrity and virulence in the phytopathogen Botrytis cinerea.
25677379	0	74	theme	/Mn	30:32	arg1	-ATPase					37:43	a P-type Ca(2+)/Mn(2+)-ATPase	15:43	a P-type Ca(2+)/Mn(2+)-ATPase mediating cell-wall integrity and virulence in the phytopathogen Botrytis cinerea	15:125	bcpmr1 encodes a P-type Ca(2+)/Mn(2+)-ATPase mediating cell-wall integrity and virulence in the phytopathogen Botrytis cinerea.
25677379	5	75	theme	glycosylation	842:854	arg1	pathway					856:862	the protein glycosylation pathway	830:862	the protein glycosylation pathway	830:862	We hypothesized that bcpmr1 also encodes a Ca(2+)/Mn(2+)-ATPase that plays an important role in the protein glycosylation pathway.
25677379	10	76	theme	B.	1572:1573	arg1	cinerea					1575:1581	B. cinerea	1572:1581	B. cinerea	1572:1581	Together, our results highlight the importance of bcpmr1 for protein glycosylation, cell wall structure and virulence of B. cinerea.
25677379	2	77	theme	O-linked	312:319	arg1	oligosaccharides					321:336	O-linked oligosaccharides	312:336	O-linked oligosaccharides	312:336	These usually contain both N- and O-linked oligosaccharides, coupled to the proteins by stepwise addition of mannose residues by mannosyltransferases in the endoplasmic reticulum and the Golgi apparatus.
25677379	7	78	theme	%	1100:1100	arg1	decrease					1102:1109	a 83% decrease	1096:1109	a 83% decrease of mannan levels	1096:1126	Significant alterations in hyphal cell wall composition were observed including a 83% decrease of mannan levels and an increase in the amount of chitin and glucan.
25677379	1	79	theme	skeletal	185:192	arg1	layer					194:198	an inner skeletal layer	176:198	an inner skeletal layer consisting of various polysaccharides surrounded by a layer of glycoproteins	176:275	The cell wall of fungi is generally composed of an inner skeletal layer consisting of various polysaccharides surrounded by a layer of glycoproteins.
28290299	3	0	theme	yellow	730:735	arg1	GCCYP					743:747	GCCYP	743:747	GCCYP	743:747	Processed field peas including conventional cooked yellow peas (CCYP), microwave cooked yellow peas (MCYP), germinated-conventional cooked yellow peas (GCCYP), and germinated-roasted yellow peas (GRYP) exhibited the significantly higher in vitro protein digestibility (IVPD), which was in accordance with their significantly lower trypsin inhibitor activity and tannin content.
28290299	3	0	theme	yellow	730:735	arg1	peas					737:740	germinated-conventional cooked yellow peas	699:740	germinated-conventional cooked yellow peas (GCCYP)	699:748	Processed field peas including conventional cooked yellow peas (CCYP), microwave cooked yellow peas (MCYP), germinated-conventional cooked yellow peas (GCCYP), and germinated-roasted yellow peas (GRYP) exhibited the significantly higher in vitro protein digestibility (IVPD), which was in accordance with their significantly lower trypsin inhibitor activity and tannin content.
28290299	7	1	theme	peas	1825:1828	arg1	functionality					1802:1814	the functionality	1798:1814	the functionality of field peas as ingredients	1798:1843	The present study would provide fundamental information to help to better understand the functionality of field peas as ingredients, and particularly in regards to agri-food industry to improve the process efficiency of field peas with enhanced nutritional and techno-functional qualities.
28290299	5	2	theme	scanning	1533:1540	arg1	image					1563:1567	the scanning electron microscopic image	1529:1567	the scanning electron microscopic image	1529:1567	The CCYP, MCYP, GCCYP, and GRYP also exhibited significantly higher starch digestibility which was supported by their lower onset (To), peak (Tp), and conclusion (Tc) temperatures obtained from DSC thermogram, their lower pasting properties and starch damage results, as well as their distinguished amorphous flakes' configuration observed on the scanning electron microscopic image.
28290299	5	3	theme	pasting	1408:1414	arg1	digestibility					1261:1273	significantly higher starch digestibility	1233:1273	significantly higher starch digestibility which was supported by their lower onset (To), peak (Tp), and conclusion (Tc) temperatures obtained from DSC thermogram	1233:1393	The CCYP, MCYP, GCCYP, and GRYP also exhibited significantly higher starch digestibility which was supported by their lower onset (To), peak (Tp), and conclusion (Tc) temperatures obtained from DSC thermogram, their lower pasting properties and starch damage results, as well as their distinguished amorphous flakes' configuration observed on the scanning electron microscopic image.
28290299	5	3	theme	pasting	1408:1414	arg1	properties					1416:1425	their lower pasting properties	1396:1425	their lower pasting properties	1396:1425	The CCYP, MCYP, GCCYP, and GRYP also exhibited significantly higher starch digestibility which was supported by their lower onset (To), peak (Tp), and conclusion (Tc) temperatures obtained from DSC thermogram, their lower pasting properties and starch damage results, as well as their distinguished amorphous flakes' configuration observed on the scanning electron microscopic image.
28290299	3	4	theme	field	601:605	arg1	peas					737:740	germinated-conventional cooked yellow peas	699:740	germinated-conventional cooked yellow peas (GCCYP)	699:748	Processed field peas including conventional cooked yellow peas (CCYP), microwave cooked yellow peas (MCYP), germinated-conventional cooked yellow peas (GCCYP), and germinated-roasted yellow peas (GRYP) exhibited the significantly higher in vitro protein digestibility (IVPD), which was in accordance with their significantly lower trypsin inhibitor activity and tannin content.
28290299	3	4	theme	field	601:605	arg1	peas					649:652	conventional cooked yellow peas	622:652	conventional cooked yellow peas (CCYP)	622:659	Processed field peas including conventional cooked yellow peas (CCYP), microwave cooked yellow peas (MCYP), germinated-conventional cooked yellow peas (GCCYP), and germinated-roasted yellow peas (GRYP) exhibited the significantly higher in vitro protein digestibility (IVPD), which was in accordance with their significantly lower trypsin inhibitor activity and tannin content.
28290299	3	4	theme	field	601:605	arg1	peas					686:689	microwave cooked yellow peas	662:689	microwave cooked yellow peas (MCYP)	662:696	Processed field peas including conventional cooked yellow peas (CCYP), microwave cooked yellow peas (MCYP), germinated-conventional cooked yellow peas (GCCYP), and germinated-roasted yellow peas (GRYP) exhibited the significantly higher in vitro protein digestibility (IVPD), which was in accordance with their significantly lower trypsin inhibitor activity and tannin content.
28290299	3	4	theme	field	601:605	arg1	peas					607:610	Processed field peas	591:610	Processed field peas including conventional cooked yellow peas (CCYP), microwave cooked yellow peas (MCYP), germinated-conventional cooked yellow peas (GCCYP), and germinated-roasted yellow peas (GRYP)	591:791	Processed field peas including conventional cooked yellow peas (CCYP), microwave cooked yellow peas (MCYP), germinated-conventional cooked yellow peas (GCCYP), and germinated-roasted yellow peas (GRYP) exhibited the significantly higher in vitro protein digestibility (IVPD), which was in accordance with their significantly lower trypsin inhibitor activity and tannin content.
28290299	3	4	theme	field	601:605	arg1	peas					781:784	germinated-roasted yellow peas	755:784	germinated-roasted yellow peas (GRYP)	755:791	Processed field peas including conventional cooked yellow peas (CCYP), microwave cooked yellow peas (MCYP), germinated-conventional cooked yellow peas (GCCYP), and germinated-roasted yellow peas (GRYP) exhibited the significantly higher in vitro protein digestibility (IVPD), which was in accordance with their significantly lower trypsin inhibitor activity and tannin content.
28290299	4	5	theme	proteins	1156:1163	arg1	hydrolysis					1138:1147	the hydrolysis	1134:1147	the hydrolysis of pea proteins to a greater extent	1134:1183	The SDS-PAGE and size exclusion HPLC profiles of untreated pea proteins and their hydrolysates also confirmed the IVPD result that these four treatments facilitated the hydrolysis of pea proteins to a greater extent.
28290299	5	6	theme	microscopic	1551:1561	arg1	image					1563:1567	the scanning electron microscopic image	1529:1567	the scanning electron microscopic image	1529:1567	The CCYP, MCYP, GCCYP, and GRYP also exhibited significantly higher starch digestibility which was supported by their lower onset (To), peak (Tp), and conclusion (Tc) temperatures obtained from DSC thermogram, their lower pasting properties and starch damage results, as well as their distinguished amorphous flakes' configuration observed on the scanning electron microscopic image.
28290299	3	7	theme	cooked	635:640	arg1	CCYP					655:658	CCYP	655:658	CCYP	655:658	Processed field peas including conventional cooked yellow peas (CCYP), microwave cooked yellow peas (MCYP), germinated-conventional cooked yellow peas (GCCYP), and germinated-roasted yellow peas (GRYP) exhibited the significantly higher in vitro protein digestibility (IVPD), which was in accordance with their significantly lower trypsin inhibitor activity and tannin content.
28290299	3	7	theme	cooked	635:640	arg1	peas					649:652	conventional cooked yellow peas	622:652	conventional cooked yellow peas (CCYP)	622:659	Processed field peas including conventional cooked yellow peas (CCYP), microwave cooked yellow peas (MCYP), germinated-conventional cooked yellow peas (GCCYP), and germinated-roasted yellow peas (GRYP) exhibited the significantly higher in vitro protein digestibility (IVPD), which was in accordance with their significantly lower trypsin inhibitor activity and tannin content.
28290299	1	8	theme	yellow	182:187	arg1	pea					195:197	yellow field pea	182:197	yellow field pea	182:197	Saskatchewan grown yellow field pea was subjected to different processing conditions including dehulling, micronization, roasting, conventional/microwave cooking, germination, and combined germination and conventional cooking/roasting.
28290299	3	9	theme	yellow	774:779	arg1	GRYP					787:790	GRYP	787:790	GRYP	787:790	Processed field peas including conventional cooked yellow peas (CCYP), microwave cooked yellow peas (MCYP), germinated-conventional cooked yellow peas (GCCYP), and germinated-roasted yellow peas (GRYP) exhibited the significantly higher in vitro protein digestibility (IVPD), which was in accordance with their significantly lower trypsin inhibitor activity and tannin content.
28290299	3	9	theme	yellow	774:779	arg1	peas					781:784	germinated-roasted yellow peas	755:784	germinated-roasted yellow peas (GRYP)	755:791	Processed field peas including conventional cooked yellow peas (CCYP), microwave cooked yellow peas (MCYP), germinated-conventional cooked yellow peas (GCCYP), and germinated-roasted yellow peas (GRYP) exhibited the significantly higher in vitro protein digestibility (IVPD), which was in accordance with their significantly lower trypsin inhibitor activity and tannin content.
28290299	6	10	theme	pea	1699:1701	arg1	proteins					1703:1710	pea proteins	1699:1710	pea proteins	1699:1710	LC/ESI-MS/MS analysis following in-gel digests of SDS-PAGE separated proteins allowed detailed compositional characterization of pea proteins.
28290299	3	11	theme	vitro	831:835	arg1	IVPD					860:863	IVPD	860:863	IVPD	860:863	Processed field peas including conventional cooked yellow peas (CCYP), microwave cooked yellow peas (MCYP), germinated-conventional cooked yellow peas (GCCYP), and germinated-roasted yellow peas (GRYP) exhibited the significantly higher in vitro protein digestibility (IVPD), which was in accordance with their significantly lower trypsin inhibitor activity and tannin content.
28290299	3	11	theme	vitro	831:835	arg1	digestibility					845:857	vitro protein digestibility	831:857	vitro protein digestibility (IVPD)	831:864	Processed field peas including conventional cooked yellow peas (CCYP), microwave cooked yellow peas (MCYP), germinated-conventional cooked yellow peas (GCCYP), and germinated-roasted yellow peas (GRYP) exhibited the significantly higher in vitro protein digestibility (IVPD), which was in accordance with their significantly lower trypsin inhibitor activity and tannin content.
28290299	3	12	theme	cooked	672:677	arg1	MCYP					692:695	MCYP	692:695	MCYP	692:695	Processed field peas including conventional cooked yellow peas (CCYP), microwave cooked yellow peas (MCYP), germinated-conventional cooked yellow peas (GCCYP), and germinated-roasted yellow peas (GRYP) exhibited the significantly higher in vitro protein digestibility (IVPD), which was in accordance with their significantly lower trypsin inhibitor activity and tannin content.
28290299	3	12	theme	cooked	672:677	arg1	peas					686:689	microwave cooked yellow peas	662:689	microwave cooked yellow peas (MCYP)	662:696	Processed field peas including conventional cooked yellow peas (CCYP), microwave cooked yellow peas (MCYP), germinated-conventional cooked yellow peas (GCCYP), and germinated-roasted yellow peas (GRYP) exhibited the significantly higher in vitro protein digestibility (IVPD), which was in accordance with their significantly lower trypsin inhibitor activity and tannin content.
28290299	7	13	theme	fundamental	1745:1755	arg1	information					1757:1767	fundamental information	1745:1767	fundamental information to help to better understand the functionality of field peas as ingredients, and particularly in regards to agri-food industry to improve the process efficiency of field peas with enhanced nutritional and techno-functional qualities	1745:2000	The present study would provide fundamental information to help to better understand the functionality of field peas as ingredients, and particularly in regards to agri-food industry to improve the process efficiency of field peas with enhanced nutritional and techno-functional qualities.
28290299	4	14	theme	HPLC	1001:1004	arg1	profiles					1006:1013	HPLC profiles	1001:1013	HPLC profiles	1001:1013	The SDS-PAGE and size exclusion HPLC profiles of untreated pea proteins and their hydrolysates also confirmed the IVPD result that these four treatments facilitated the hydrolysis of pea proteins to a greater extent.
28290299	6	15	theme	detailed	1656:1663	arg1	characterization					1679:1694	detailed compositional characterization	1656:1694	detailed compositional characterization of pea proteins	1656:1710	LC/ESI-MS/MS analysis following in-gel digests of SDS-PAGE separated proteins allowed detailed compositional characterization of pea proteins.
28290299	5	16	theme	onset	1310:1314	arg1	temperatures					1353:1364	their lower onset (To), peak (Tp), and conclusion (Tc) temperatures	1298:1364	their lower onset (To), peak (Tp), and conclusion (Tc) temperatures obtained from DSC thermogram	1298:1393	The CCYP, MCYP, GCCYP, and GRYP also exhibited significantly higher starch digestibility which was supported by their lower onset (To), peak (Tp), and conclusion (Tc) temperatures obtained from DSC thermogram, their lower pasting properties and starch damage results, as well as their distinguished amorphous flakes' configuration observed on the scanning electron microscopic image.
28290299	5	16	theme	onset	1310:1314	arg1	Tc					1349:1350	Tc	1349:1350	Tc	1349:1350	The CCYP, MCYP, GCCYP, and GRYP also exhibited significantly higher starch digestibility which was supported by their lower onset (To), peak (Tp), and conclusion (Tc) temperatures obtained from DSC thermogram, their lower pasting properties and starch damage results, as well as their distinguished amorphous flakes' configuration observed on the scanning electron microscopic image.
28290299	2	17	theme	starch	531:536	arg1	digestibility					538:550	starch digestibility	531:550	starch digestibility	531:550	Their nutritional and antinutritional compositions, functional properties, microstructure, thermal properties, in vitro protein and starch digestibility, and protein composition were studied.
28290299	2	17	theme	starch	531:536	arg1	properties					462:471	functional properties	451:471	functional properties	451:471	Their nutritional and antinutritional compositions, functional properties, microstructure, thermal properties, in vitro protein and starch digestibility, and protein composition were studied.
28290299	0	18	theme	Saskatchewan	80:91	arg1	Pisum					118:122	Pisum	118:122	Pisum	118:122	In vitro digestibility, protein composition and techno-functional properties of Saskatchewan grown yellow field peas (Pisum sativum L.) as affected by processing.
28290299	0	18	theme	Saskatchewan	80:91	arg1	peas					112:115	Saskatchewan grown yellow field peas	80:115	Saskatchewan grown yellow field peas (Pisum sativum L.)	80:134	In vitro digestibility, protein composition and techno-functional properties of Saskatchewan grown yellow field peas (Pisum sativum L.) as affected by processing.
28290299	0	19	dep	Pisum	118:122	arg1	L.					132:133	Pisum sativum L.	118:133	Pisum sativum L.	118:133	In vitro digestibility, protein composition and techno-functional properties of Saskatchewan grown yellow field peas (Pisum sativum L.) as affected by processing.
28290299	7	20	theme	present	1717:1723	arg1	study					1725:1729	The present study	1713:1729	The present study	1713:1729	The present study would provide fundamental information to help to better understand the functionality of field peas as ingredients, and particularly in regards to agri-food industry to improve the process efficiency of field peas with enhanced nutritional and techno-functional qualities.
28290299	0	21	theme	yellow	99:104	arg1	Pisum					118:122	Pisum	118:122	Pisum	118:122	In vitro digestibility, protein composition and techno-functional properties of Saskatchewan grown yellow field peas (Pisum sativum L.) as affected by processing.
28290299	0	21	theme	yellow	99:104	arg1	peas					112:115	Saskatchewan grown yellow field peas	80:115	Saskatchewan grown yellow field peas (Pisum sativum L.)	80:134	In vitro digestibility, protein composition and techno-functional properties of Saskatchewan grown yellow field peas (Pisum sativum L.) as affected by processing.
28290299	1	22	theme	conventional	368:379	arg1	cooking/roasting					381:396	conventional cooking/roasting	368:396	conventional cooking/roasting	368:396	Saskatchewan grown yellow field pea was subjected to different processing conditions including dehulling, micronization, roasting, conventional/microwave cooking, germination, and combined germination and conventional cooking/roasting.
28290299	6	23	theme	separated	1629:1637	arg1	proteins					1639:1646	SDS-PAGE separated proteins	1620:1646	SDS-PAGE separated proteins	1620:1646	LC/ESI-MS/MS analysis following in-gel digests of SDS-PAGE separated proteins allowed detailed compositional characterization of pea proteins.
28290299	3	24	theme	inhibitor	930:938	arg1	activity					940:947	their significantly lower trypsin inhibitor activity	896:947	their significantly lower trypsin inhibitor activity	896:947	Processed field peas including conventional cooked yellow peas (CCYP), microwave cooked yellow peas (MCYP), germinated-conventional cooked yellow peas (GCCYP), and germinated-roasted yellow peas (GRYP) exhibited the significantly higher in vitro protein digestibility (IVPD), which was in accordance with their significantly lower trypsin inhibitor activity and tannin content.
28290299	2	25	theme	protein	557:563	arg1	composition					565:575	protein composition	557:575	protein composition	557:575	Their nutritional and antinutritional compositions, functional properties, microstructure, thermal properties, in vitro protein and starch digestibility, and protein composition were studied.
28290299	0	26	theme	peas	112:115	arg1	digestibility					9:21	In vitro digestibility	0:21	In vitro digestibility	0:21	In vitro digestibility, protein composition and techno-functional properties of Saskatchewan grown yellow field peas (Pisum sativum L.) as affected by processing.
28290299	0	26	theme	peas	112:115	arg1	composition					32:42	protein composition	24:42	protein composition	24:42	In vitro digestibility, protein composition and techno-functional properties of Saskatchewan grown yellow field peas (Pisum sativum L.) as affected by processing.
28290299	0	26	theme	peas	112:115	arg1	properties					66:75	techno-functional properties	48:75	techno-functional properties	48:75	In vitro digestibility, protein composition and techno-functional properties of Saskatchewan grown yellow field peas (Pisum sativum L.) as affected by processing.
28290299	7	27	theme	enhanced	1949:1956	arg1	qualities					1992:2000	enhanced nutritional and techno-functional qualities	1949:2000	enhanced nutritional and techno-functional qualities	1949:2000	The present study would provide fundamental information to help to better understand the functionality of field peas as ingredients, and particularly in regards to agri-food industry to improve the process efficiency of field peas with enhanced nutritional and techno-functional qualities.
28290299	3	28	theme	lower	916:920	arg1	activity					940:947	their significantly lower trypsin inhibitor activity	896:947	their significantly lower trypsin inhibitor activity	896:947	Processed field peas including conventional cooked yellow peas (CCYP), microwave cooked yellow peas (MCYP), germinated-conventional cooked yellow peas (GCCYP), and germinated-roasted yellow peas (GRYP) exhibited the significantly higher in vitro protein digestibility (IVPD), which was in accordance with their significantly lower trypsin inhibitor activity and tannin content.
28290299	4	29	theme	hydrolysates	1051:1062	arg1	SDS-PAGE					973:980	SDS-PAGE	973:980	SDS-PAGE	973:980	The SDS-PAGE and size exclusion HPLC profiles of untreated pea proteins and their hydrolysates also confirmed the IVPD result that these four treatments facilitated the hydrolysis of pea proteins to a greater extent.
28290299	4	29	theme	hydrolysates	1051:1062	arg1	exclusion					991:999	size exclusion	986:999	size exclusion	986:999	The SDS-PAGE and size exclusion HPLC profiles of untreated pea proteins and their hydrolysates also confirmed the IVPD result that these four treatments facilitated the hydrolysis of pea proteins to a greater extent.
28290299	5	30	theme	damage	1438:1443	arg1	digestibility					1261:1273	significantly higher starch digestibility	1233:1273	significantly higher starch digestibility which was supported by their lower onset (To), peak (Tp), and conclusion (Tc) temperatures obtained from DSC thermogram	1233:1393	The CCYP, MCYP, GCCYP, and GRYP also exhibited significantly higher starch digestibility which was supported by their lower onset (To), peak (Tp), and conclusion (Tc) temperatures obtained from DSC thermogram, their lower pasting properties and starch damage results, as well as their distinguished amorphous flakes' configuration observed on the scanning electron microscopic image.
28290299	5	30	theme	damage	1438:1443	arg1	results					1445:1451	starch damage results	1431:1451	starch damage results	1431:1451	The CCYP, MCYP, GCCYP, and GRYP also exhibited significantly higher starch digestibility which was supported by their lower onset (To), peak (Tp), and conclusion (Tc) temperatures obtained from DSC thermogram, their lower pasting properties and starch damage results, as well as their distinguished amorphous flakes' configuration observed on the scanning electron microscopic image.
28290299	5	31	located	observed	1517:1524	arg2	configuration					1503:1515	their distinguished amorphous flakes' configuration	1465:1515	significantly higher starch digestibility which was supported by their lower onset (To), peak (Tp), and conclusion (Tc) temperatures obtained from DSC thermogram, their lower pasting properties and starch damage results, as well as their distinguished amorphous flakes' configuration observed on the scanning electron microscopic image	1233:1567	The CCYP, MCYP, GCCYP, and GRYP also exhibited significantly higher starch digestibility which was supported by their lower onset (To), peak (Tp), and conclusion (Tc) temperatures obtained from DSC thermogram, their lower pasting properties and starch damage results, as well as their distinguished amorphous flakes' configuration observed on the scanning electron microscopic image.
28290299	5	31	located	observed	1517:1524	arg1	image					1563:1567	the scanning electron microscopic image	1529:1567	the scanning electron microscopic image	1529:1567	The CCYP, MCYP, GCCYP, and GRYP also exhibited significantly higher starch digestibility which was supported by their lower onset (To), peak (Tp), and conclusion (Tc) temperatures obtained from DSC thermogram, their lower pasting properties and starch damage results, as well as their distinguished amorphous flakes' configuration observed on the scanning electron microscopic image.
28290299	0	32	theme	In	0:1	arg1	digestibility					9:21	In vitro digestibility	0:21	In vitro digestibility	0:21	In vitro digestibility, protein composition and techno-functional properties of Saskatchewan grown yellow field peas (Pisum sativum L.) as affected by processing.
28290299	7	33	theme	peas	1939:1942	arg1	efficiency					1919:1928	the process efficiency	1907:1928	the process efficiency of field peas with enhanced nutritional and techno-functional qualities	1907:2000	The present study would provide fundamental information to help to better understand the functionality of field peas as ingredients, and particularly in regards to agri-food industry to improve the process efficiency of field peas with enhanced nutritional and techno-functional qualities.
28290299	6	34	theme	in-gel	1602:1607	arg1	digests					1609:1615	in-gel digests	1602:1615	in-gel digests of SDS-PAGE separated proteins	1602:1646	LC/ESI-MS/MS analysis following in-gel digests of SDS-PAGE separated proteins allowed detailed compositional characterization of pea proteins.
28290299	4	35	theme	pea	1028:1030	arg1	proteins					1032:1039	untreated pea proteins	1018:1039	untreated pea proteins	1018:1039	The SDS-PAGE and size exclusion HPLC profiles of untreated pea proteins and their hydrolysates also confirmed the IVPD result that these four treatments facilitated the hydrolysis of pea proteins to a greater extent.
28290299	2	36	theme	functional	451:460	arg1	digestibility					538:550	starch digestibility	531:550	starch digestibility	531:550	Their nutritional and antinutritional compositions, functional properties, microstructure, thermal properties, in vitro protein and starch digestibility, and protein composition were studied.
28290299	2	36	theme	functional	451:460	arg1	microstructure					474:487	microstructure	474:487	microstructure	474:487	Their nutritional and antinutritional compositions, functional properties, microstructure, thermal properties, in vitro protein and starch digestibility, and protein composition were studied.
28290299	2	36	theme	functional	451:460	arg1	properties					498:507	thermal properties	490:507	thermal properties	490:507	Their nutritional and antinutritional compositions, functional properties, microstructure, thermal properties, in vitro protein and starch digestibility, and protein composition were studied.
28290299	2	36	theme	functional	451:460	arg1	properties					462:471	functional properties	451:471	functional properties	451:471	Their nutritional and antinutritional compositions, functional properties, microstructure, thermal properties, in vitro protein and starch digestibility, and protein composition were studied.
28290299	2	36	theme	functional	451:460	arg1	protein					519:525	in vitro protein	510:525	in vitro protein	510:525	Their nutritional and antinutritional compositions, functional properties, microstructure, thermal properties, in vitro protein and starch digestibility, and protein composition were studied.
28290299	0	37	theme	protein	24:30	arg1	composition					32:42	protein composition	24:42	protein composition	24:42	In vitro digestibility, protein composition and techno-functional properties of Saskatchewan grown yellow field peas (Pisum sativum L.) as affected by processing.
28290299	7	38	theme	process	1911:1917	arg1	efficiency					1919:1928	the process efficiency	1907:1928	the process efficiency of field peas with enhanced nutritional and techno-functional qualities	1907:2000	The present study would provide fundamental information to help to better understand the functionality of field peas as ingredients, and particularly in regards to agri-food industry to improve the process efficiency of field peas with enhanced nutritional and techno-functional qualities.
28290299	2	39	theme	in	510:511	arg1	properties					462:471	functional properties	451:471	functional properties	451:471	Their nutritional and antinutritional compositions, functional properties, microstructure, thermal properties, in vitro protein and starch digestibility, and protein composition were studied.
28290299	2	39	theme	in	510:511	arg1	protein					519:525	in vitro protein	510:525	in vitro protein	510:525	Their nutritional and antinutritional compositions, functional properties, microstructure, thermal properties, in vitro protein and starch digestibility, and protein composition were studied.
28290299	4	40	theme	size	986:989	arg1	exclusion					991:999	size exclusion	986:999	size exclusion	986:999	The SDS-PAGE and size exclusion HPLC profiles of untreated pea proteins and their hydrolysates also confirmed the IVPD result that these four treatments facilitated the hydrolysis of pea proteins to a greater extent.
28290299	5	41	theme	amorphous	1485:1493	arg1	configuration					1503:1515	their distinguished amorphous flakes' configuration	1465:1515	significantly higher starch digestibility which was supported by their lower onset (To), peak (Tp), and conclusion (Tc) temperatures obtained from DSC thermogram, their lower pasting properties and starch damage results, as well as their distinguished amorphous flakes' configuration observed on the scanning electron microscopic image	1233:1567	The CCYP, MCYP, GCCYP, and GRYP also exhibited significantly higher starch digestibility which was supported by their lower onset (To), peak (Tp), and conclusion (Tc) temperatures obtained from DSC thermogram, their lower pasting properties and starch damage results, as well as their distinguished amorphous flakes' configuration observed on the scanning electron microscopic image.
28290299	3	42	theme	cooked	723:728	arg1	GCCYP					743:747	GCCYP	743:747	GCCYP	743:747	Processed field peas including conventional cooked yellow peas (CCYP), microwave cooked yellow peas (MCYP), germinated-conventional cooked yellow peas (GCCYP), and germinated-roasted yellow peas (GRYP) exhibited the significantly higher in vitro protein digestibility (IVPD), which was in accordance with their significantly lower trypsin inhibitor activity and tannin content.
28290299	3	42	theme	cooked	723:728	arg1	peas					737:740	germinated-conventional cooked yellow peas	699:740	germinated-conventional cooked yellow peas (GCCYP)	699:748	Processed field peas including conventional cooked yellow peas (CCYP), microwave cooked yellow peas (MCYP), germinated-conventional cooked yellow peas (GCCYP), and germinated-roasted yellow peas (GRYP) exhibited the significantly higher in vitro protein digestibility (IVPD), which was in accordance with their significantly lower trypsin inhibitor activity and tannin content.
28290299	5	43	theme	higher	1247:1252	arg1	digestibility					1261:1273	significantly higher starch digestibility	1233:1273	significantly higher starch digestibility which was supported by their lower onset (To), peak (Tp), and conclusion (Tc) temperatures obtained from DSC thermogram	1233:1393	The CCYP, MCYP, GCCYP, and GRYP also exhibited significantly higher starch digestibility which was supported by their lower onset (To), peak (Tp), and conclusion (Tc) temperatures obtained from DSC thermogram, their lower pasting properties and starch damage results, as well as their distinguished amorphous flakes' configuration observed on the scanning electron microscopic image.
28290299	5	43	theme	higher	1247:1252	arg1	results					1445:1451	starch damage results	1431:1451	starch damage results	1431:1451	The CCYP, MCYP, GCCYP, and GRYP also exhibited significantly higher starch digestibility which was supported by their lower onset (To), peak (Tp), and conclusion (Tc) temperatures obtained from DSC thermogram, their lower pasting properties and starch damage results, as well as their distinguished amorphous flakes' configuration observed on the scanning electron microscopic image.
28290299	5	43	theme	higher	1247:1252	arg1	properties					1416:1425	their lower pasting properties	1396:1425	their lower pasting properties	1396:1425	The CCYP, MCYP, GCCYP, and GRYP also exhibited significantly higher starch digestibility which was supported by their lower onset (To), peak (Tp), and conclusion (Tc) temperatures obtained from DSC thermogram, their lower pasting properties and starch damage results, as well as their distinguished amorphous flakes' configuration observed on the scanning electron microscopic image.
28290299	0	44	dep	In	0:1	arg1	vitro					3:7	vitro	3:7	vitro	3:7	In vitro digestibility, protein composition and techno-functional properties of Saskatchewan grown yellow field peas (Pisum sativum L.) as affected by processing.
28290299	4	45	theme	IVPD	1083:1086	arg1	result					1088:1093	the IVPD result	1079:1093	the IVPD result	1079:1093	The SDS-PAGE and size exclusion HPLC profiles of untreated pea proteins and their hydrolysates also confirmed the IVPD result that these four treatments facilitated the hydrolysis of pea proteins to a greater extent.
28290299	7	46	theme	field	1819:1823	arg1	peas					1825:1828	field peas	1819:1828	field peas as ingredients	1819:1843	The present study would provide fundamental information to help to better understand the functionality of field peas as ingredients, and particularly in regards to agri-food industry to improve the process efficiency of field peas with enhanced nutritional and techno-functional qualities.
28290299	7	47	dep	understand	1787:1796	arg1	improve					1899:1905	improve	1899:1905	to improve the process efficiency of field peas with enhanced nutritional and techno-functional qualities	1896:2000	The present study would provide fundamental information to help to better understand the functionality of field peas as ingredients, and particularly in regards to agri-food industry to improve the process efficiency of field peas with enhanced nutritional and techno-functional qualities.
28290299	7	48	theme	techno-functional	1974:1990	arg1	qualities					1992:2000	enhanced nutritional and techno-functional qualities	1949:2000	enhanced nutritional and techno-functional qualities	1949:2000	The present study would provide fundamental information to help to better understand the functionality of field peas as ingredients, and particularly in regards to agri-food industry to improve the process efficiency of field peas with enhanced nutritional and techno-functional qualities.
28290299	5	49	theme	lower	1402:1406	arg1	digestibility					1261:1273	significantly higher starch digestibility	1233:1273	significantly higher starch digestibility which was supported by their lower onset (To), peak (Tp), and conclusion (Tc) temperatures obtained from DSC thermogram	1233:1393	The CCYP, MCYP, GCCYP, and GRYP also exhibited significantly higher starch digestibility which was supported by their lower onset (To), peak (Tp), and conclusion (Tc) temperatures obtained from DSC thermogram, their lower pasting properties and starch damage results, as well as their distinguished amorphous flakes' configuration observed on the scanning electron microscopic image.
28290299	5	49	theme	lower	1402:1406	arg1	properties					1416:1425	their lower pasting properties	1396:1425	their lower pasting properties	1396:1425	The CCYP, MCYP, GCCYP, and GRYP also exhibited significantly higher starch digestibility which was supported by their lower onset (To), peak (Tp), and conclusion (Tc) temperatures obtained from DSC thermogram, their lower pasting properties and starch damage results, as well as their distinguished amorphous flakes' configuration observed on the scanning electron microscopic image.
28290299	3	50	theme	Processed	591:599	arg1	peas					737:740	germinated-conventional cooked yellow peas	699:740	germinated-conventional cooked yellow peas (GCCYP)	699:748	Processed field peas including conventional cooked yellow peas (CCYP), microwave cooked yellow peas (MCYP), germinated-conventional cooked yellow peas (GCCYP), and germinated-roasted yellow peas (GRYP) exhibited the significantly higher in vitro protein digestibility (IVPD), which was in accordance with their significantly lower trypsin inhibitor activity and tannin content.
28290299	3	50	theme	Processed	591:599	arg1	peas					649:652	conventional cooked yellow peas	622:652	conventional cooked yellow peas (CCYP)	622:659	Processed field peas including conventional cooked yellow peas (CCYP), microwave cooked yellow peas (MCYP), germinated-conventional cooked yellow peas (GCCYP), and germinated-roasted yellow peas (GRYP) exhibited the significantly higher in vitro protein digestibility (IVPD), which was in accordance with their significantly lower trypsin inhibitor activity and tannin content.
28290299	3	50	theme	Processed	591:599	arg1	peas					686:689	microwave cooked yellow peas	662:689	microwave cooked yellow peas (MCYP)	662:696	Processed field peas including conventional cooked yellow peas (CCYP), microwave cooked yellow peas (MCYP), germinated-conventional cooked yellow peas (GCCYP), and germinated-roasted yellow peas (GRYP) exhibited the significantly higher in vitro protein digestibility (IVPD), which was in accordance with their significantly lower trypsin inhibitor activity and tannin content.
28290299	3	50	theme	Processed	591:599	arg1	peas					607:610	Processed field peas	591:610	Processed field peas including conventional cooked yellow peas (CCYP), microwave cooked yellow peas (MCYP), germinated-conventional cooked yellow peas (GCCYP), and germinated-roasted yellow peas (GRYP)	591:791	Processed field peas including conventional cooked yellow peas (CCYP), microwave cooked yellow peas (MCYP), germinated-conventional cooked yellow peas (GCCYP), and germinated-roasted yellow peas (GRYP) exhibited the significantly higher in vitro protein digestibility (IVPD), which was in accordance with their significantly lower trypsin inhibitor activity and tannin content.
28290299	3	50	theme	Processed	591:599	arg1	peas					781:784	germinated-roasted yellow peas	755:784	germinated-roasted yellow peas (GRYP)	755:791	Processed field peas including conventional cooked yellow peas (CCYP), microwave cooked yellow peas (MCYP), germinated-conventional cooked yellow peas (GCCYP), and germinated-roasted yellow peas (GRYP) exhibited the significantly higher in vitro protein digestibility (IVPD), which was in accordance with their significantly lower trypsin inhibitor activity and tannin content.
28290299	5	51	theme	electron	1542:1549	arg1	image					1563:1567	the scanning electron microscopic image	1529:1567	the scanning electron microscopic image	1529:1567	The CCYP, MCYP, GCCYP, and GRYP also exhibited significantly higher starch digestibility which was supported by their lower onset (To), peak (Tp), and conclusion (Tc) temperatures obtained from DSC thermogram, their lower pasting properties and starch damage results, as well as their distinguished amorphous flakes' configuration observed on the scanning electron microscopic image.
28290299	3	52	theme	germinated-roasted	755:772	arg1	GRYP					787:790	GRYP	787:790	GRYP	787:790	Processed field peas including conventional cooked yellow peas (CCYP), microwave cooked yellow peas (MCYP), germinated-conventional cooked yellow peas (GCCYP), and germinated-roasted yellow peas (GRYP) exhibited the significantly higher in vitro protein digestibility (IVPD), which was in accordance with their significantly lower trypsin inhibitor activity and tannin content.
28290299	3	52	theme	germinated-roasted	755:772	arg1	peas					781:784	germinated-roasted yellow peas	755:784	germinated-roasted yellow peas (GRYP)	755:791	Processed field peas including conventional cooked yellow peas (CCYP), microwave cooked yellow peas (MCYP), germinated-conventional cooked yellow peas (GCCYP), and germinated-roasted yellow peas (GRYP) exhibited the significantly higher in vitro protein digestibility (IVPD), which was in accordance with their significantly lower trypsin inhibitor activity and tannin content.
28290299	4	53	theme	greater	1170:1176	arg1	extent					1178:1183	a greater extent	1168:1183	a greater extent	1168:1183	The SDS-PAGE and size exclusion HPLC profiles of untreated pea proteins and their hydrolysates also confirmed the IVPD result that these four treatments facilitated the hydrolysis of pea proteins to a greater extent.
28290299	5	54	theme	DSC	1380:1382	arg1	thermogram					1384:1393	DSC thermogram	1380:1393	DSC thermogram	1380:1393	The CCYP, MCYP, GCCYP, and GRYP also exhibited significantly higher starch digestibility which was supported by their lower onset (To), peak (Tp), and conclusion (Tc) temperatures obtained from DSC thermogram, their lower pasting properties and starch damage results, as well as their distinguished amorphous flakes' configuration observed on the scanning electron microscopic image.
28290299	3	55	theme	conventional	622:633	arg1	CCYP					655:658	CCYP	655:658	CCYP	655:658	Processed field peas including conventional cooked yellow peas (CCYP), microwave cooked yellow peas (MCYP), germinated-conventional cooked yellow peas (GCCYP), and germinated-roasted yellow peas (GRYP) exhibited the significantly higher in vitro protein digestibility (IVPD), which was in accordance with their significantly lower trypsin inhibitor activity and tannin content.
28290299	3	55	theme	conventional	622:633	arg1	peas					649:652	conventional cooked yellow peas	622:652	conventional cooked yellow peas (CCYP)	622:659	Processed field peas including conventional cooked yellow peas (CCYP), microwave cooked yellow peas (MCYP), germinated-conventional cooked yellow peas (GCCYP), and germinated-roasted yellow peas (GRYP) exhibited the significantly higher in vitro protein digestibility (IVPD), which was in accordance with their significantly lower trypsin inhibitor activity and tannin content.
28290299	1	56	theme	field	189:193	arg1	pea					195:197	yellow field pea	182:197	yellow field pea	182:197	Saskatchewan grown yellow field pea was subjected to different processing conditions including dehulling, micronization, roasting, conventional/microwave cooking, germination, and combined germination and conventional cooking/roasting.
28290299	4	57	theme	pea	1152:1154	arg1	proteins					1156:1163	pea proteins	1152:1163	pea proteins	1152:1163	The SDS-PAGE and size exclusion HPLC profiles of untreated pea proteins and their hydrolysates also confirmed the IVPD result that these four treatments facilitated the hydrolysis of pea proteins to a greater extent.
28290299	4	58	dep	SDS-PAGE	973:980	arg1	The					969:971	The	969:971	The	969:971	The SDS-PAGE and size exclusion HPLC profiles of untreated pea proteins and their hydrolysates also confirmed the IVPD result that these four treatments facilitated the hydrolysis of pea proteins to a greater extent.
28290299	4	58	dep	SDS-PAGE	973:980	arg1	profiles					1006:1013	HPLC profiles	1001:1013	HPLC profiles	1001:1013	The SDS-PAGE and size exclusion HPLC profiles of untreated pea proteins and their hydrolysates also confirmed the IVPD result that these four treatments facilitated the hydrolysis of pea proteins to a greater extent.
28290299	6	59	theme	proteins	1703:1710	arg1	characterization					1679:1694	detailed compositional characterization	1656:1694	detailed compositional characterization of pea proteins	1656:1710	LC/ESI-MS/MS analysis following in-gel digests of SDS-PAGE separated proteins allowed detailed compositional characterization of pea proteins.
28290299	3	60	theme	yellow	642:647	arg1	CCYP					655:658	CCYP	655:658	CCYP	655:658	Processed field peas including conventional cooked yellow peas (CCYP), microwave cooked yellow peas (MCYP), germinated-conventional cooked yellow peas (GCCYP), and germinated-roasted yellow peas (GRYP) exhibited the significantly higher in vitro protein digestibility (IVPD), which was in accordance with their significantly lower trypsin inhibitor activity and tannin content.
28290299	3	60	theme	yellow	642:647	arg1	peas					649:652	conventional cooked yellow peas	622:652	conventional cooked yellow peas (CCYP)	622:659	Processed field peas including conventional cooked yellow peas (CCYP), microwave cooked yellow peas (MCYP), germinated-conventional cooked yellow peas (GCCYP), and germinated-roasted yellow peas (GRYP) exhibited the significantly higher in vitro protein digestibility (IVPD), which was in accordance with their significantly lower trypsin inhibitor activity and tannin content.
28290299	6	61	theme	LC/ESI-MS/MS	1570:1581	arg1	analysis					1583:1590	LC/ESI-MS/MS analysis	1570:1590	LC/ESI-MS/MS analysis following in-gel digests of SDS-PAGE separated proteins	1570:1646	LC/ESI-MS/MS analysis following in-gel digests of SDS-PAGE separated proteins allowed detailed compositional characterization of pea proteins.
28290299	7	62	with	peas	1939:1942	arg1	qualities					1992:2000	enhanced nutritional and techno-functional qualities	1949:2000	enhanced nutritional and techno-functional qualities	1949:2000	The present study would provide fundamental information to help to better understand the functionality of field peas as ingredients, and particularly in regards to agri-food industry to improve the process efficiency of field peas with enhanced nutritional and techno-functional qualities.
28290299	3	63	theme	microwave	662:670	arg1	MCYP					692:695	MCYP	692:695	MCYP	692:695	Processed field peas including conventional cooked yellow peas (CCYP), microwave cooked yellow peas (MCYP), germinated-conventional cooked yellow peas (GCCYP), and germinated-roasted yellow peas (GRYP) exhibited the significantly higher in vitro protein digestibility (IVPD), which was in accordance with their significantly lower trypsin inhibitor activity and tannin content.
28290299	3	63	theme	microwave	662:670	arg1	peas					686:689	microwave cooked yellow peas	662:689	microwave cooked yellow peas (MCYP)	662:696	Processed field peas including conventional cooked yellow peas (CCYP), microwave cooked yellow peas (MCYP), germinated-conventional cooked yellow peas (GCCYP), and germinated-roasted yellow peas (GRYP) exhibited the significantly higher in vitro protein digestibility (IVPD), which was in accordance with their significantly lower trypsin inhibitor activity and tannin content.
28290299	6	64	theme	compositional	1665:1677	arg1	characterization					1679:1694	detailed compositional characterization	1656:1694	detailed compositional characterization of pea proteins	1656:1710	LC/ESI-MS/MS analysis following in-gel digests of SDS-PAGE separated proteins allowed detailed compositional characterization of pea proteins.
28290299	5	65	theme	lower	1304:1308	arg1	onset					1310:1314	their lower onset	1298:1314	their lower onset (To)	1298:1319	The CCYP, MCYP, GCCYP, and GRYP also exhibited significantly higher starch digestibility which was supported by their lower onset (To), peak (Tp), and conclusion (Tc) temperatures obtained from DSC thermogram, their lower pasting properties and starch damage results, as well as their distinguished amorphous flakes' configuration observed on the scanning electron microscopic image.
28290299	3	66	theme	protein	837:843	arg1	IVPD					860:863	IVPD	860:863	IVPD	860:863	Processed field peas including conventional cooked yellow peas (CCYP), microwave cooked yellow peas (MCYP), germinated-conventional cooked yellow peas (GCCYP), and germinated-roasted yellow peas (GRYP) exhibited the significantly higher in vitro protein digestibility (IVPD), which was in accordance with their significantly lower trypsin inhibitor activity and tannin content.
28290299	3	66	theme	protein	837:843	arg1	digestibility					845:857	vitro protein digestibility	831:857	vitro protein digestibility (IVPD)	831:864	Processed field peas including conventional cooked yellow peas (CCYP), microwave cooked yellow peas (MCYP), germinated-conventional cooked yellow peas (GCCYP), and germinated-roasted yellow peas (GRYP) exhibited the significantly higher in vitro protein digestibility (IVPD), which was in accordance with their significantly lower trypsin inhibitor activity and tannin content.
28290299	0	67	theme	grown	93:97	arg1	Pisum					118:122	Pisum	118:122	Pisum	118:122	In vitro digestibility, protein composition and techno-functional properties of Saskatchewan grown yellow field peas (Pisum sativum L.) as affected by processing.
28290299	0	67	theme	grown	93:97	arg1	peas					112:115	Saskatchewan grown yellow field peas	80:115	Saskatchewan grown yellow field peas (Pisum sativum L.)	80:134	In vitro digestibility, protein composition and techno-functional properties of Saskatchewan grown yellow field peas (Pisum sativum L.) as affected by processing.
28290299	1	68	theme	combined	343:350	arg1	germination					352:362	combined germination	343:362	combined germination	343:362	Saskatchewan grown yellow field pea was subjected to different processing conditions including dehulling, micronization, roasting, conventional/microwave cooking, germination, and combined germination and conventional cooking/roasting.
28290299	2	69	dep	in	510:511	arg1	vitro					513:517	vitro	513:517	vitro	513:517	Their nutritional and antinutritional compositions, functional properties, microstructure, thermal properties, in vitro protein and starch digestibility, and protein composition were studied.
28290299	0	70	theme	field	106:110	arg1	Pisum					118:122	Pisum	118:122	Pisum	118:122	In vitro digestibility, protein composition and techno-functional properties of Saskatchewan grown yellow field peas (Pisum sativum L.) as affected by processing.
28290299	0	70	theme	field	106:110	arg1	peas					112:115	Saskatchewan grown yellow field peas	80:115	Saskatchewan grown yellow field peas (Pisum sativum L.)	80:134	In vitro digestibility, protein composition and techno-functional properties of Saskatchewan grown yellow field peas (Pisum sativum L.) as affected by processing.
28290299	7	71	theme	nutritional	1958:1968	arg1	qualities					1992:2000	enhanced nutritional and techno-functional qualities	1949:2000	enhanced nutritional and techno-functional qualities	1949:2000	The present study would provide fundamental information to help to better understand the functionality of field peas as ingredients, and particularly in regards to agri-food industry to improve the process efficiency of field peas with enhanced nutritional and techno-functional qualities.
28290299	6	72	theme	proteins	1639:1646	arg1	digests					1609:1615	in-gel digests	1602:1615	in-gel digests of SDS-PAGE separated proteins	1602:1646	LC/ESI-MS/MS analysis following in-gel digests of SDS-PAGE separated proteins allowed detailed compositional characterization of pea proteins.
28290299	5	73	theme	peak	1322:1325	arg1	temperatures					1353:1364	their lower onset (To), peak (Tp), and conclusion (Tc) temperatures	1298:1364	their lower onset (To), peak (Tp), and conclusion (Tc) temperatures obtained from DSC thermogram	1298:1393	The CCYP, MCYP, GCCYP, and GRYP also exhibited significantly higher starch digestibility which was supported by their lower onset (To), peak (Tp), and conclusion (Tc) temperatures obtained from DSC thermogram, their lower pasting properties and starch damage results, as well as their distinguished amorphous flakes' configuration observed on the scanning electron microscopic image.
28290299	5	73	theme	peak	1322:1325	arg1	Tc					1349:1350	Tc	1349:1350	Tc	1349:1350	The CCYP, MCYP, GCCYP, and GRYP also exhibited significantly higher starch digestibility which was supported by their lower onset (To), peak (Tp), and conclusion (Tc) temperatures obtained from DSC thermogram, their lower pasting properties and starch damage results, as well as their distinguished amorphous flakes' configuration observed on the scanning electron microscopic image.
28290299	6	74	theme	SDS-PAGE	1620:1627	arg1	proteins					1639:1646	SDS-PAGE separated proteins	1620:1646	SDS-PAGE separated proteins	1620:1646	LC/ESI-MS/MS analysis following in-gel digests of SDS-PAGE separated proteins allowed detailed compositional characterization of pea proteins.
28290299	5	75	theme	starch	1431:1436	arg1	digestibility					1261:1273	significantly higher starch digestibility	1233:1273	significantly higher starch digestibility which was supported by their lower onset (To), peak (Tp), and conclusion (Tc) temperatures obtained from DSC thermogram	1233:1393	The CCYP, MCYP, GCCYP, and GRYP also exhibited significantly higher starch digestibility which was supported by their lower onset (To), peak (Tp), and conclusion (Tc) temperatures obtained from DSC thermogram, their lower pasting properties and starch damage results, as well as their distinguished amorphous flakes' configuration observed on the scanning electron microscopic image.
28290299	5	75	theme	starch	1431:1436	arg1	results					1445:1451	starch damage results	1431:1451	starch damage results	1431:1451	The CCYP, MCYP, GCCYP, and GRYP also exhibited significantly higher starch digestibility which was supported by their lower onset (To), peak (Tp), and conclusion (Tc) temperatures obtained from DSC thermogram, their lower pasting properties and starch damage results, as well as their distinguished amorphous flakes' configuration observed on the scanning electron microscopic image.
28290299	2	76	theme	antinutritional	421:435	arg1	compositions					437:448	Their nutritional and antinutritional compositions	399:448	Their nutritional and antinutritional compositions	399:448	Their nutritional and antinutritional compositions, functional properties, microstructure, thermal properties, in vitro protein and starch digestibility, and protein composition were studied.
28290299	7	77	theme	field	1933:1937	arg1	peas					1939:1942	field peas	1933:1942	field peas with enhanced nutritional and techno-functional qualities	1933:2000	The present study would provide fundamental information to help to better understand the functionality of field peas as ingredients, and particularly in regards to agri-food industry to improve the process efficiency of field peas with enhanced nutritional and techno-functional qualities.
28290299	3	78	theme	trypsin	922:928	arg1	activity					940:947	their significantly lower trypsin inhibitor activity	896:947	their significantly lower trypsin inhibitor activity	896:947	Processed field peas including conventional cooked yellow peas (CCYP), microwave cooked yellow peas (MCYP), germinated-conventional cooked yellow peas (GCCYP), and germinated-roasted yellow peas (GRYP) exhibited the significantly higher in vitro protein digestibility (IVPD), which was in accordance with their significantly lower trypsin inhibitor activity and tannin content.
28290299	2	79	theme	thermal	490:496	arg1	properties					498:507	thermal properties	490:507	thermal properties	490:507	Their nutritional and antinutritional compositions, functional properties, microstructure, thermal properties, in vitro protein and starch digestibility, and protein composition were studied.
28290299	2	79	theme	thermal	490:496	arg1	properties					462:471	functional properties	451:471	functional properties	451:471	Their nutritional and antinutritional compositions, functional properties, microstructure, thermal properties, in vitro protein and starch digestibility, and protein composition were studied.
28290299	2	80	theme	nutritional	405:415	arg1	compositions					437:448	Their nutritional and antinutritional compositions	399:448	Their nutritional and antinutritional compositions	399:448	Their nutritional and antinutritional compositions, functional properties, microstructure, thermal properties, in vitro protein and starch digestibility, and protein composition were studied.
28290299	1	81	theme	conventional/microwave	294:315	arg1	cooking					317:323	conventional/microwave cooking	294:323	conventional/microwave cooking	294:323	Saskatchewan grown yellow field pea was subjected to different processing conditions including dehulling, micronization, roasting, conventional/microwave cooking, germination, and combined germination and conventional cooking/roasting.
28290299	3	82	theme	tannin	953:958	arg1	content					960:966	tannin content	953:966	tannin content	953:966	Processed field peas including conventional cooked yellow peas (CCYP), microwave cooked yellow peas (MCYP), germinated-conventional cooked yellow peas (GCCYP), and germinated-roasted yellow peas (GRYP) exhibited the significantly higher in vitro protein digestibility (IVPD), which was in accordance with their significantly lower trypsin inhibitor activity and tannin content.
28290299	4	83	theme	untreated	1018:1026	arg1	proteins					1032:1039	untreated pea proteins	1018:1039	untreated pea proteins	1018:1039	The SDS-PAGE and size exclusion HPLC profiles of untreated pea proteins and their hydrolysates also confirmed the IVPD result that these four treatments facilitated the hydrolysis of pea proteins to a greater extent.
28290299	3	84	theme	yellow	679:684	arg1	MCYP					692:695	MCYP	692:695	MCYP	692:695	Processed field peas including conventional cooked yellow peas (CCYP), microwave cooked yellow peas (MCYP), germinated-conventional cooked yellow peas (GCCYP), and germinated-roasted yellow peas (GRYP) exhibited the significantly higher in vitro protein digestibility (IVPD), which was in accordance with their significantly lower trypsin inhibitor activity and tannin content.
28290299	3	84	theme	yellow	679:684	arg1	peas					686:689	microwave cooked yellow peas	662:689	microwave cooked yellow peas (MCYP)	662:696	Processed field peas including conventional cooked yellow peas (CCYP), microwave cooked yellow peas (MCYP), germinated-conventional cooked yellow peas (GCCYP), and germinated-roasted yellow peas (GRYP) exhibited the significantly higher in vitro protein digestibility (IVPD), which was in accordance with their significantly lower trypsin inhibitor activity and tannin content.
28290299	1	85	theme	processing	226:235	arg1	germination					326:336	germination	326:336	germination	326:336	Saskatchewan grown yellow field pea was subjected to different processing conditions including dehulling, micronization, roasting, conventional/microwave cooking, germination, and combined germination and conventional cooking/roasting.
28290299	1	85	theme	processing	226:235	arg1	cooking/roasting					381:396	conventional cooking/roasting	368:396	conventional cooking/roasting	368:396	Saskatchewan grown yellow field pea was subjected to different processing conditions including dehulling, micronization, roasting, conventional/microwave cooking, germination, and combined germination and conventional cooking/roasting.
28290299	1	85	theme	processing	226:235	arg1	conditions					237:246	different processing conditions	216:246	different processing conditions including dehulling, micronization, roasting, conventional/microwave cooking, germination, and combined germination and conventional cooking/roasting	216:396	Saskatchewan grown yellow field pea was subjected to different processing conditions including dehulling, micronization, roasting, conventional/microwave cooking, germination, and combined germination and conventional cooking/roasting.
28290299	1	85	theme	processing	226:235	arg1	roasting					284:291	roasting	284:291	roasting	284:291	Saskatchewan grown yellow field pea was subjected to different processing conditions including dehulling, micronization, roasting, conventional/microwave cooking, germination, and combined germination and conventional cooking/roasting.
28290299	1	85	theme	processing	226:235	arg1	germination					352:362	combined germination	343:362	combined germination	343:362	Saskatchewan grown yellow field pea was subjected to different processing conditions including dehulling, micronization, roasting, conventional/microwave cooking, germination, and combined germination and conventional cooking/roasting.
28290299	1	85	theme	processing	226:235	arg1	dehulling					258:266	dehulling	258:266	dehulling	258:266	Saskatchewan grown yellow field pea was subjected to different processing conditions including dehulling, micronization, roasting, conventional/microwave cooking, germination, and combined germination and conventional cooking/roasting.
28290299	1	85	theme	processing	226:235	arg1	cooking					317:323	conventional/microwave cooking	294:323	conventional/microwave cooking	294:323	Saskatchewan grown yellow field pea was subjected to different processing conditions including dehulling, micronization, roasting, conventional/microwave cooking, germination, and combined germination and conventional cooking/roasting.
28290299	1	85	theme	processing	226:235	arg1	micronization					269:281	micronization	269:281	micronization	269:281	Saskatchewan grown yellow field pea was subjected to different processing conditions including dehulling, micronization, roasting, conventional/microwave cooking, germination, and combined germination and conventional cooking/roasting.
28290299	0	86	theme	techno-functional	48:64	arg1	properties					66:75	techno-functional properties	48:75	techno-functional properties	48:75	In vitro digestibility, protein composition and techno-functional properties of Saskatchewan grown yellow field peas (Pisum sativum L.) as affected by processing.
28290299	4	87	theme	proteins	1032:1039	arg1	SDS-PAGE					973:980	SDS-PAGE	973:980	SDS-PAGE	973:980	The SDS-PAGE and size exclusion HPLC profiles of untreated pea proteins and their hydrolysates also confirmed the IVPD result that these four treatments facilitated the hydrolysis of pea proteins to a greater extent.
28290299	4	87	theme	proteins	1032:1039	arg1	exclusion					991:999	size exclusion	986:999	size exclusion	986:999	The SDS-PAGE and size exclusion HPLC profiles of untreated pea proteins and their hydrolysates also confirmed the IVPD result that these four treatments facilitated the hydrolysis of pea proteins to a greater extent.
28290299	5	88	theme	distinguished	1471:1483	arg1	configuration					1503:1515	their distinguished amorphous flakes' configuration	1465:1515	significantly higher starch digestibility which was supported by their lower onset (To), peak (Tp), and conclusion (Tc) temperatures obtained from DSC thermogram, their lower pasting properties and starch damage results, as well as their distinguished amorphous flakes' configuration observed on the scanning electron microscopic image	1233:1567	The CCYP, MCYP, GCCYP, and GRYP also exhibited significantly higher starch digestibility which was supported by their lower onset (To), peak (Tp), and conclusion (Tc) temperatures obtained from DSC thermogram, their lower pasting properties and starch damage results, as well as their distinguished amorphous flakes' configuration observed on the scanning electron microscopic image.
28290299	3	89	theme	germinated-conventional	699:721	arg1	GCCYP					743:747	GCCYP	743:747	GCCYP	743:747	Processed field peas including conventional cooked yellow peas (CCYP), microwave cooked yellow peas (MCYP), germinated-conventional cooked yellow peas (GCCYP), and germinated-roasted yellow peas (GRYP) exhibited the significantly higher in vitro protein digestibility (IVPD), which was in accordance with their significantly lower trypsin inhibitor activity and tannin content.
28290299	3	89	theme	germinated-conventional	699:721	arg1	peas					737:740	germinated-conventional cooked yellow peas	699:740	germinated-conventional cooked yellow peas (GCCYP)	699:748	Processed field peas including conventional cooked yellow peas (CCYP), microwave cooked yellow peas (MCYP), germinated-conventional cooked yellow peas (GCCYP), and germinated-roasted yellow peas (GRYP) exhibited the significantly higher in vitro protein digestibility (IVPD), which was in accordance with their significantly lower trypsin inhibitor activity and tannin content.
28290299	7	90	theme	agri-food	1877:1885	arg1	industry					1887:1894	agri-food industry	1877:1894	agri-food industry	1877:1894	The present study would provide fundamental information to help to better understand the functionality of field peas as ingredients, and particularly in regards to agri-food industry to improve the process efficiency of field peas with enhanced nutritional and techno-functional qualities.
28290299	1	91	theme	different	216:224	arg1	germination					326:336	germination	326:336	germination	326:336	Saskatchewan grown yellow field pea was subjected to different processing conditions including dehulling, micronization, roasting, conventional/microwave cooking, germination, and combined germination and conventional cooking/roasting.
28290299	1	91	theme	different	216:224	arg1	cooking/roasting					381:396	conventional cooking/roasting	368:396	conventional cooking/roasting	368:396	Saskatchewan grown yellow field pea was subjected to different processing conditions including dehulling, micronization, roasting, conventional/microwave cooking, germination, and combined germination and conventional cooking/roasting.
28290299	1	91	theme	different	216:224	arg1	conditions					237:246	different processing conditions	216:246	different processing conditions including dehulling, micronization, roasting, conventional/microwave cooking, germination, and combined germination and conventional cooking/roasting	216:396	Saskatchewan grown yellow field pea was subjected to different processing conditions including dehulling, micronization, roasting, conventional/microwave cooking, germination, and combined germination and conventional cooking/roasting.
28290299	1	91	theme	different	216:224	arg1	roasting					284:291	roasting	284:291	roasting	284:291	Saskatchewan grown yellow field pea was subjected to different processing conditions including dehulling, micronization, roasting, conventional/microwave cooking, germination, and combined germination and conventional cooking/roasting.
28290299	1	91	theme	different	216:224	arg1	germination					352:362	combined germination	343:362	combined germination	343:362	Saskatchewan grown yellow field pea was subjected to different processing conditions including dehulling, micronization, roasting, conventional/microwave cooking, germination, and combined germination and conventional cooking/roasting.
28290299	1	91	theme	different	216:224	arg1	dehulling					258:266	dehulling	258:266	dehulling	258:266	Saskatchewan grown yellow field pea was subjected to different processing conditions including dehulling, micronization, roasting, conventional/microwave cooking, germination, and combined germination and conventional cooking/roasting.
28290299	1	91	theme	different	216:224	arg1	cooking					317:323	conventional/microwave cooking	294:323	conventional/microwave cooking	294:323	Saskatchewan grown yellow field pea was subjected to different processing conditions including dehulling, micronization, roasting, conventional/microwave cooking, germination, and combined germination and conventional cooking/roasting.
28290299	1	91	theme	different	216:224	arg1	micronization					269:281	micronization	269:281	micronization	269:281	Saskatchewan grown yellow field pea was subjected to different processing conditions including dehulling, micronization, roasting, conventional/microwave cooking, germination, and combined germination and conventional cooking/roasting.
28290299	5	92	theme	conclusion	1337:1346	arg1	temperatures					1353:1364	their lower onset (To), peak (Tp), and conclusion (Tc) temperatures	1298:1364	their lower onset (To), peak (Tp), and conclusion (Tc) temperatures obtained from DSC thermogram	1298:1393	The CCYP, MCYP, GCCYP, and GRYP also exhibited significantly higher starch digestibility which was supported by their lower onset (To), peak (Tp), and conclusion (Tc) temperatures obtained from DSC thermogram, their lower pasting properties and starch damage results, as well as their distinguished amorphous flakes' configuration observed on the scanning electron microscopic image.
28290299	5	92	theme	conclusion	1337:1346	arg1	Tc					1349:1350	Tc	1349:1350	Tc	1349:1350	The CCYP, MCYP, GCCYP, and GRYP also exhibited significantly higher starch digestibility which was supported by their lower onset (To), peak (Tp), and conclusion (Tc) temperatures obtained from DSC thermogram, their lower pasting properties and starch damage results, as well as their distinguished amorphous flakes' configuration observed on the scanning electron microscopic image.
28290299	5	93	theme	starch	1254:1259	arg1	digestibility					1261:1273	significantly higher starch digestibility	1233:1273	significantly higher starch digestibility which was supported by their lower onset (To), peak (Tp), and conclusion (Tc) temperatures obtained from DSC thermogram	1233:1393	The CCYP, MCYP, GCCYP, and GRYP also exhibited significantly higher starch digestibility which was supported by their lower onset (To), peak (Tp), and conclusion (Tc) temperatures obtained from DSC thermogram, their lower pasting properties and starch damage results, as well as their distinguished amorphous flakes' configuration observed on the scanning electron microscopic image.
28290299	5	93	theme	starch	1254:1259	arg1	results					1445:1451	starch damage results	1431:1451	starch damage results	1431:1451	The CCYP, MCYP, GCCYP, and GRYP also exhibited significantly higher starch digestibility which was supported by their lower onset (To), peak (Tp), and conclusion (Tc) temperatures obtained from DSC thermogram, their lower pasting properties and starch damage results, as well as their distinguished amorphous flakes' configuration observed on the scanning electron microscopic image.
28290299	5	93	theme	starch	1254:1259	arg1	properties					1416:1425	their lower pasting properties	1396:1425	their lower pasting properties	1396:1425	The CCYP, MCYP, GCCYP, and GRYP also exhibited significantly higher starch digestibility which was supported by their lower onset (To), peak (Tp), and conclusion (Tc) temperatures obtained from DSC thermogram, their lower pasting properties and starch damage results, as well as their distinguished amorphous flakes' configuration observed on the scanning electron microscopic image.
27287148	4	0	theme	incubation	675:684	arg1	period					691:696	the incubation time period	671:696	the incubation time period of 3, 7, and 14days	671:716	Finally, the cell viability tests were carried out (MTT) over the incubation time period of 3, 7, and 14days.
27287148	1	1	theme	preparation	185:195	arg1	parameters					197:206	preparation parameters	185:206	preparation parameters	185:206	In the present study alginate-brushite composite hydrogels were in-situ synthetized and characterized with respect to preparation parameters.
27287148	9	2	theme	samples	1283:1289	arg1	majority					1271:1278	the majority	1267:1278	the majority of samples especially those prepared in the acidic conditions	1267:1340	Also, a considerable impact of pH variation on the biocompatibility of samples was noticed so that the majority of samples especially those prepared in the acidic conditions were toxic.
27287148	9	2	theme	samples	1283:1289	arg1	toxic					1347:1351	toxic	1347:1351	toxic	1347:1351	Also, a considerable impact of pH variation on the biocompatibility of samples was noticed so that the majority of samples especially those prepared in the acidic conditions were toxic.
27287148	7	3	with	accordance	1028:1037	arg1	those					1044:1048	those	1044:1048	those	1044:1048	Besides, the XRD and thermal analysis results were in a good accordance with those of FTIR.
27287148	7	4	theme	good	1023:1026	arg1	accordance					1028:1037	a good accordance	1021:1037	a good accordance with those of FTIR	1021:1056	Besides, the XRD and thermal analysis results were in a good accordance with those of FTIR.
27287148	9	5	theme	considerable	1176:1187	arg1	impact					1189:1194	a considerable impact	1174:1194	a considerable impact of pH variation on the biocompatibility of samples	1174:1245	Also, a considerable impact of pH variation on the biocompatibility of samples was noticed so that the majority of samples especially those prepared in the acidic conditions were toxic.
27287148	6	6	theme	characteristics	903:917	arg1	peaks					919:923	characteristics peaks	903:923	characteristics peaks of brushite	903:935	It was shown that as the pH reached to the value of 6, characteristics peaks of brushite appeared in the FTIR spectra.
27287148	2	7	from	influence	227:235	arg1	fabrication					321:331	the in-situ fabrication	309:331	the in-situ fabrication of the composite hydrogel	309:357	Specifically, the influence of initial pH value and initial concentration of phosphate precursor on the in-situ fabrication of the composite hydrogel were taken into account.
27287148	4	8	theme	14days	711:716	arg1	period					691:696	the incubation time period	671:696	the incubation time period of 3, 7, and 14days	671:716	Finally, the cell viability tests were carried out (MTT) over the incubation time period of 3, 7, and 14days.
27287148	1	9	theme	present	74:80	arg1	study					82:86	the present study	70:86	the present study	70:86	In the present study alginate-brushite composite hydrogels were in-situ synthetized and characterized with respect to preparation parameters.
27287148	8	10	theme	SEM	1076:1078	arg1	images					1080:1085	the SEM images	1072:1085	the SEM images	1072:1085	In addition, the SEM images demonstrated that the plate like brushite was formed inside the alginate matrix.
27287148	5	11	theme	brushite	792:799	arg1	formation					749:757	the formation	745:757	the formation	745:757	The results revealed that the formation and the crystalline stability of brushite were highly dependent on the initial pH value.
27287148	5	11	theme	brushite	792:799	arg1	dependent					813:821	dependent	813:821	dependent	813:821	The results revealed that the formation and the crystalline stability of brushite were highly dependent on the initial pH value.
27287148	5	11	theme	brushite	792:799	arg1	stability					779:787	the crystalline stability	763:787	the crystalline stability of brushite	763:799	The results revealed that the formation and the crystalline stability of brushite were highly dependent on the initial pH value.
27287148	4	12	theme	7	704:704	arg1	period					691:696	the incubation time period	671:696	the incubation time period of 3, 7, and 14days	671:716	Finally, the cell viability tests were carried out (MTT) over the incubation time period of 3, 7, and 14days.
27287148	2	13	theme	in-situ	313:319	arg1	fabrication					321:331	the in-situ fabrication	309:331	the in-situ fabrication of the composite hydrogel	309:357	Specifically, the influence of initial pH value and initial concentration of phosphate precursor on the in-situ fabrication of the composite hydrogel were taken into account.
27287148	3	14	theme	composite	388:396	arg1	hydrogels					398:406	The composite hydrogels	384:406	The composite hydrogels	384:406	The composite hydrogels were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric (TGA, DTG) and differential thermal analysis (DTA).
27287148	9	15	theme	pH	1199:1200	arg1	variation					1202:1210	pH variation	1199:1210	pH variation	1199:1210	Also, a considerable impact of pH variation on the biocompatibility of samples was noticed so that the majority of samples especially those prepared in the acidic conditions were toxic.
27287148	1	16	theme	alginate-brushite	88:104	arg1	hydrogels					116:124	alginate-brushite composite hydrogels	88:124	alginate-brushite composite hydrogels	88:124	In the present study alginate-brushite composite hydrogels were in-situ synthetized and characterized with respect to preparation parameters.
27287148	3	17	theme	X-ray	430:434	arg1	XRD					449:451	XRD	449:451	XRD	449:451	The composite hydrogels were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric (TGA, DTG) and differential thermal analysis (DTA).
27287148	3	17	theme	X-ray	430:434	arg1	Fourier					455:461	Fourier	455:461	Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric (TGA, DTG) and differential thermal analysis (DTA)	455:606	The composite hydrogels were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric (TGA, DTG) and differential thermal analysis (DTA).
27287148	3	17	theme	X-ray	430:434	arg1	diffraction					436:446	X-ray diffraction	430:446	X-ray diffraction (XRD)	430:452	The composite hydrogels were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric (TGA, DTG) and differential thermal analysis (DTA).
27287148	9	18	theme	variation	1202:1210	arg1	impact					1189:1194	a considerable impact	1174:1194	a considerable impact of pH variation on the biocompatibility of samples	1174:1245	Also, a considerable impact of pH variation on the biocompatibility of samples was noticed so that the majority of samples especially those prepared in the acidic conditions were toxic.
27287148	7	19	theme	thermal	988:994	arg1	results					1005:1011	the XRD and thermal analysis results	976:1011	the XRD and thermal analysis results	976:1011	Besides, the XRD and thermal analysis results were in a good accordance with those of FTIR.
27287148	1	20	theme	composite	106:114	arg1	hydrogels					116:124	alginate-brushite composite hydrogels	88:124	alginate-brushite composite hydrogels	88:124	In the present study alginate-brushite composite hydrogels were in-situ synthetized and characterized with respect to preparation parameters.
27287148	9	21	theme	acidic	1324:1329	arg1	conditions					1331:1340	the acidic conditions	1320:1340	the acidic conditions	1320:1340	Also, a considerable impact of pH variation on the biocompatibility of samples was noticed so that the majority of samples especially those prepared in the acidic conditions were toxic.
27287148	6	22	theme	6	900:900	arg1	value					891:895	the value	887:895	the value of 6	887:900	It was shown that as the pH reached to the value of 6, characteristics peaks of brushite appeared in the FTIR spectra.
27287148	7	23	theme	analysis	996:1003	arg1	results					1005:1011	the XRD and thermal analysis results	976:1011	the XRD and thermal analysis results	976:1011	Besides, the XRD and thermal analysis results were in a good accordance with those of FTIR.
27287148	2	24	theme	hydrogel	350:357	arg1	fabrication					321:331	the in-situ fabrication	309:331	the in-situ fabrication of the composite hydrogel	309:357	Specifically, the influence of initial pH value and initial concentration of phosphate precursor on the in-situ fabrication of the composite hydrogel were taken into account.
27287148	5	25	theme	initial	830:836	arg1	value					841:845	the initial pH value	826:845	the initial pH value	826:845	The results revealed that the formation and the crystalline stability of brushite were highly dependent on the initial pH value.
27287148	0	26	theme	in-situ	38:44	arg1	composites					55:64	alginate-brushite in-situ hydrogel composites	20:64	alginate-brushite in-situ hydrogel composites	20:64	Characterization of alginate-brushite in-situ hydrogel composites.
27287148	7	27	theme	XRD	980:982	arg1	results					1005:1011	the XRD and thermal analysis results	976:1011	the XRD and thermal analysis results	976:1011	Besides, the XRD and thermal analysis results were in a good accordance with those of FTIR.
27287148	2	28	theme	composite	340:348	arg1	hydrogel					350:357	the composite hydrogel	336:357	the composite hydrogel	336:357	Specifically, the influence of initial pH value and initial concentration of phosphate precursor on the in-situ fabrication of the composite hydrogel were taken into account.
27287148	3	29	dep	Fourier	455:461	arg1	transform					463:471	transform	463:471	transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric (TGA, DTG) and differential thermal analysis (DTA)	463:606	The composite hydrogels were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric (TGA, DTG) and differential thermal analysis (DTA).
27287148	4	30	theme	viability	627:635	arg1	tests					637:641	the cell viability tests	618:641	the cell viability tests	618:641	Finally, the cell viability tests were carried out (MTT) over the incubation time period of 3, 7, and 14days.
27287148	0	31	theme	alginate-brushite	20:36	arg1	composites					55:64	alginate-brushite in-situ hydrogel composites	20:64	alginate-brushite in-situ hydrogel composites	20:64	Characterization of alginate-brushite in-situ hydrogel composites.
27287148	3	32	dep	thermogravimetric	539:555	arg1	DTG					563:565	DTG	563:565	DTG	563:565	The composite hydrogels were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric (TGA, DTG) and differential thermal analysis (DTA).
27287148	3	32	dep	thermogravimetric	539:555	arg1	TGA					558:560	TGA	558:560	TGA	558:560	The composite hydrogels were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric (TGA, DTG) and differential thermal analysis (DTA).
27287148	4	33	theme	cell	622:625	arg1	tests					637:641	the cell viability tests	618:641	the cell viability tests	618:641	Finally, the cell viability tests were carried out (MTT) over the incubation time period of 3, 7, and 14days.
27287148	0	34	theme	composites	55:64	arg1	Characterization					0:15	Characterization	0:15	Characterization of alginate-brushite in-situ hydrogel composites.	0:65	Characterization of alginate-brushite in-situ hydrogel composites.
27287148	2	35	theme	concentration	269:281	arg1	influence					227:235	the influence	223:235	the influence of initial pH value and initial concentration of phosphate precursor on the in-situ fabrication of the composite hydrogel	223:357	Specifically, the influence of initial pH value and initial concentration of phosphate precursor on the in-situ fabrication of the composite hydrogel were taken into account.
27287148	0	36	theme	hydrogel	46:53	arg1	composites					55:64	alginate-brushite in-situ hydrogel composites	20:64	alginate-brushite in-situ hydrogel composites	20:64	Characterization of alginate-brushite in-situ hydrogel composites.
27287148	9	37	theme	samples	1239:1245	arg1	biocompatibility					1219:1234	the biocompatibility	1215:1234	the biocompatibility of samples	1215:1245	Also, a considerable impact of pH variation on the biocompatibility of samples was noticed so that the majority of samples especially those prepared in the acidic conditions were toxic.
27287148	2	38	theme	initial	261:267	arg1	concentration					269:281	initial concentration	261:281	initial concentration of phosphate precursor	261:304	Specifically, the influence of initial pH value and initial concentration of phosphate precursor on the in-situ fabrication of the composite hydrogel were taken into account.
27287148	5	39	theme	crystalline	767:777	arg1	dependent					813:821	dependent	813:821	dependent	813:821	The results revealed that the formation and the crystalline stability of brushite were highly dependent on the initial pH value.
27287148	5	39	theme	crystalline	767:777	arg1	stability					779:787	the crystalline stability	763:787	the crystalline stability of brushite	763:799	The results revealed that the formation and the crystalline stability of brushite were highly dependent on the initial pH value.
27287148	2	40	theme	pH	248:249	arg1	value					251:255	initial pH value	240:255	initial pH value	240:255	Specifically, the influence of initial pH value and initial concentration of phosphate precursor on the in-situ fabrication of the composite hydrogel were taken into account.
27287148	2	41	theme	value	251:255	arg1	influence					227:235	the influence	223:235	the influence of initial pH value and initial concentration of phosphate precursor on the in-situ fabrication of the composite hydrogel	223:357	Specifically, the influence of initial pH value and initial concentration of phosphate precursor on the in-situ fabrication of the composite hydrogel were taken into account.
27287148	4	42	theme	time	686:689	arg1	period					691:696	the incubation time period	671:696	the incubation time period of 3, 7, and 14days	671:716	Finally, the cell viability tests were carried out (MTT) over the incubation time period of 3, 7, and 14days.
27287148	3	43	theme	differential	572:583	arg1	DTA					603:605	DTA	603:605	DTA	603:605	The composite hydrogels were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric (TGA, DTG) and differential thermal analysis (DTA).
27287148	3	43	theme	differential	572:583	arg1	analysis					593:600	differential thermal analysis	572:600	differential thermal analysis (DTA)	572:606	The composite hydrogels were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric (TGA, DTG) and differential thermal analysis (DTA).
27287148	2	44	theme	initial	240:246	arg1	value					251:255	initial pH value	240:255	initial pH value	240:255	Specifically, the influence of initial pH value and initial concentration of phosphate precursor on the in-situ fabrication of the composite hydrogel were taken into account.
27287148	3	45	dep	transform	463:471	arg1	infrared					473:480	infrared	473:480	transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric (TGA, DTG) and differential thermal analysis (DTA)	463:606	The composite hydrogels were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric (TGA, DTG) and differential thermal analysis (DTA).
27287148	3	46	theme	scanning	503:510	arg1	microscopy					521:530	scanning electron microscopy	503:530	scanning electron microscopy (SEM)	503:536	The composite hydrogels were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric (TGA, DTG) and differential thermal analysis (DTA).
27287148	3	46	theme	scanning	503:510	arg1	SEM					533:535	SEM	533:535	SEM	533:535	The composite hydrogels were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric (TGA, DTG) and differential thermal analysis (DTA).
27287148	9	47	dep	samples	1283:1289	arg1	those					1302:1306	those	1302:1306	those	1302:1306	Also, a considerable impact of pH variation on the biocompatibility of samples was noticed so that the majority of samples especially those prepared in the acidic conditions were toxic.
27287148	2	48	theme	precursor	296:304	arg1	value					251:255	initial pH value	240:255	initial pH value	240:255	Specifically, the influence of initial pH value and initial concentration of phosphate precursor on the in-situ fabrication of the composite hydrogel were taken into account.
27287148	2	48	theme	precursor	296:304	arg1	concentration					269:281	initial concentration	261:281	initial concentration of phosphate precursor	261:304	Specifically, the influence of initial pH value and initial concentration of phosphate precursor on the in-situ fabrication of the composite hydrogel were taken into account.
27287148	3	49	theme	electron	512:519	arg1	microscopy					521:530	scanning electron microscopy	503:530	scanning electron microscopy (SEM)	503:536	The composite hydrogels were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric (TGA, DTG) and differential thermal analysis (DTA).
27287148	3	49	theme	electron	512:519	arg1	SEM					533:535	SEM	533:535	SEM	533:535	The composite hydrogels were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric (TGA, DTG) and differential thermal analysis (DTA).
27287148	4	50	theme	3	701:701	arg1	period					691:696	the incubation time period	671:696	the incubation time period of 3, 7, and 14days	671:716	Finally, the cell viability tests were carried out (MTT) over the incubation time period of 3, 7, and 14days.
27287148	6	51	theme	FTIR	953:956	arg1	spectra					958:964	the FTIR spectra	949:964	the FTIR spectra	949:964	It was shown that as the pH reached to the value of 6, characteristics peaks of brushite appeared in the FTIR spectra.
27287148	2	52	theme	phosphate	286:294	arg1	precursor					296:304	phosphate precursor	286:304	phosphate precursor	286:304	Specifically, the influence of initial pH value and initial concentration of phosphate precursor on the in-situ fabrication of the composite hydrogel were taken into account.
27287148	8	53	theme	alginate	1151:1158	arg1	matrix					1160:1165	the alginate matrix	1147:1165	the alginate matrix	1147:1165	In addition, the SEM images demonstrated that the plate like brushite was formed inside the alginate matrix.
27287148	5	54	theme	pH	838:839	arg1	value					841:845	the initial pH value	826:845	the initial pH value	826:845	The results revealed that the formation and the crystalline stability of brushite were highly dependent on the initial pH value.
27287148	9	55	from	impact	1189:1194	arg1	biocompatibility					1219:1234	the biocompatibility	1215:1234	the biocompatibility of samples	1215:1245	Also, a considerable impact of pH variation on the biocompatibility of samples was noticed so that the majority of samples especially those prepared in the acidic conditions were toxic.
27287148	4	56	dep	carried	648:654	arg1	MTT					661:663	MTT	661:663	MTT	661:663	Finally, the cell viability tests were carried out (MTT) over the incubation time period of 3, 7, and 14days.
27287148	3	57	theme	thermal	585:591	arg1	DTA					603:605	DTA	603:605	DTA	603:605	The composite hydrogels were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric (TGA, DTG) and differential thermal analysis (DTA).
27287148	3	57	theme	thermal	585:591	arg1	analysis					593:600	differential thermal analysis	572:600	differential thermal analysis (DTA)	572:606	The composite hydrogels were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric (TGA, DTG) and differential thermal analysis (DTA).
27287148	6	58	theme	brushite	928:935	arg1	peaks					919:923	characteristics peaks	903:923	characteristics peaks of brushite	903:935	It was shown that as the pH reached to the value of 6, characteristics peaks of brushite appeared in the FTIR spectra.
28554813	8	0	theme	L-EPS	1433:1437	arg1	specific					1452:1459	specific	1452:1459	specific	1452:1459	Both live and L-EPS of L. gasseri strains were capable of inhibiting the cell proliferation of HeLa cells with the impact of L-EPS being strain specific.
28554813	8	0	theme	L-EPS	1433:1437	arg1	impact					1423:1428	the impact	1419:1428	the impact of L-EPS	1419:1437	Both live and L-EPS of L. gasseri strains were capable of inhibiting the cell proliferation of HeLa cells with the impact of L-EPS being strain specific.
28554813	6	1	theme	individual	1166:1175	arg1	monosaccharides					1177:1191	the individual monosaccharides	1162:1191	the individual monosaccharides	1162:1191	The sugar component was the same; however, relative proportions of the individual monosaccharides except mannose were different.
28554813	8	2	theme	cells	1408:1412	arg1	proliferation					1386:1398	the cell proliferation	1377:1398	the cell proliferation of HeLa cells	1377:1412	Both live and L-EPS of L. gasseri strains were capable of inhibiting the cell proliferation of HeLa cells with the impact of L-EPS being strain specific.
28554813	8	3	theme	HeLa	1403:1406	arg1	cells					1408:1412	HeLa cells	1403:1412	HeLa cells	1403:1412	Both live and L-EPS of L. gasseri strains were capable of inhibiting the cell proliferation of HeLa cells with the impact of L-EPS being strain specific.
28554813	2	4	theme	cervical	441:448	arg1	cancer					450:455	cervical cancer	441:455	cervical cancer	441:455	Exopolysaccharides (EPSs) produced by lactobacillus have been found to have an important role in probiotic activity; however, there is limited knowledge concerning their impact on cervical cancer and urogenital health.
28554813	11	5	theme	L. gasseri	1646:1655	arg1	strains					1657:1663	L. gasseri strains	1646:1663	L. gasseri strains	1646:1663	L. gasseri strains showed an anti-inflammatory impact on HeLa cells by decreasing the production of TNF-α and increasing the IL-10 production.
28554813	4	6	theme	increasing	775:784	arg1	L-EPS					820:824	L-EPS	820:824	L-EPS	820:824	HeLa cells were treated with live culture at ∼108 CFU/ml or increasing concentration of lyophilized EPS (L-EPS) (100, 200, or 400 μg/ml) of L. gasseri strains and their ability to adhere to host cells, inhibit proliferation, and modulate immune response were evaluated.
28554813	4	6	theme	increasing	775:784	arg1	concentration					786:798	increasing concentration	775:798	increasing concentration of lyophilized EPS (L-EPS) (100, 200, or 400 μg/ml) of L. gasseri strains and their ability to adhere to host cells, inhibit proliferation, and modulate immune response	775:967	HeLa cells were treated with live culture at ∼108 CFU/ml or increasing concentration of lyophilized EPS (L-EPS) (100, 200, or 400 μg/ml) of L. gasseri strains and their ability to adhere to host cells, inhibit proliferation, and modulate immune response were evaluated.
28554813	4	6	theme	increasing	775:784	arg1	400 μg/ml					841:849	100, 200, or 400 μg/ml	828:849	100, 200, or 400 μg/ml	828:849	HeLa cells were treated with live culture at ∼108 CFU/ml or increasing concentration of lyophilized EPS (L-EPS) (100, 200, or 400 μg/ml) of L. gasseri strains and their ability to adhere to host cells, inhibit proliferation, and modulate immune response were evaluated.
28554813	8	7	theme	cell	1381:1384	arg1	proliferation					1386:1398	the cell proliferation	1377:1398	the cell proliferation of HeLa cells	1377:1412	Both live and L-EPS of L. gasseri strains were capable of inhibiting the cell proliferation of HeLa cells with the impact of L-EPS being strain specific.
28554813	4	8	theme	lyophilized	803:813	arg1	EPS					815:817	lyophilized EPS	803:817	lyophilized EPS	803:817	HeLa cells were treated with live culture at ∼108 CFU/ml or increasing concentration of lyophilized EPS (L-EPS) (100, 200, or 400 μg/ml) of L. gasseri strains and their ability to adhere to host cells, inhibit proliferation, and modulate immune response were evaluated.
28554813	9	9	theme	L. gasseri	1472:1481	arg1	strains					1483:1489	L. gasseri strains	1472:1489	L. gasseri strains	1472:1489	L-EPSs of L. gasseri strains induced apoptosis in HeLa cells in a strain dependent manner.
28554813	3	10	theme	study	502:506	arg1	objective					484:492	The objective	480:492	The objective of this study	480:506	The objective of this study is to investigate and compare EPSs of L. gasseri strains (G10 and H15), isolated from a healthy human vagina, for their capability to inhibit cervical cancer cell (HeLa) growth and modulate immune response.
28554813	4	11	theme	strains	866:872	arg1	L-EPS					820:824	L-EPS	820:824	L-EPS	820:824	HeLa cells were treated with live culture at ∼108 CFU/ml or increasing concentration of lyophilized EPS (L-EPS) (100, 200, or 400 μg/ml) of L. gasseri strains and their ability to adhere to host cells, inhibit proliferation, and modulate immune response were evaluated.
28554813	4	11	theme	strains	866:872	arg1	concentration					786:798	increasing concentration	775:798	increasing concentration of lyophilized EPS (L-EPS) (100, 200, or 400 μg/ml) of L. gasseri strains and their ability to adhere to host cells, inhibit proliferation, and modulate immune response	775:967	HeLa cells were treated with live culture at ∼108 CFU/ml or increasing concentration of lyophilized EPS (L-EPS) (100, 200, or 400 μg/ml) of L. gasseri strains and their ability to adhere to host cells, inhibit proliferation, and modulate immune response were evaluated.
28554813	4	11	theme	strains	866:872	arg1	400 μg/ml					841:849	100, 200, or 400 μg/ml	828:849	100, 200, or 400 μg/ml	828:849	HeLa cells were treated with live culture at ∼108 CFU/ml or increasing concentration of lyophilized EPS (L-EPS) (100, 200, or 400 μg/ml) of L. gasseri strains and their ability to adhere to host cells, inhibit proliferation, and modulate immune response were evaluated.
28554813	0	12	theme	human	83:87	arg1	vagina					89:94	human vagina	83:94	human vagina on cervical tumor cells (HeLa)	83:125	Impact of Exopolysaccharides (EPSs) of Lactobacillus gasseri strains isolated from human vagina on cervical tumor cells (HeLa).
28554813	3	13	theme	cervical	650:657	arg1	growth					678:683	cervical cancer cell (HeLa) growth	650:683	cervical cancer cell (HeLa) growth	650:683	The objective of this study is to investigate and compare EPSs of L. gasseri strains (G10 and H15), isolated from a healthy human vagina, for their capability to inhibit cervical cancer cell (HeLa) growth and modulate immune response.
28554813	13	14	theme	strain	2146:2151	arg1	activity					2181:2188	strain specific anti-proliferative activity	2146:2188	strain specific anti-proliferative activity	2146:2188	Although our results suggest a relationship between the ability of a strain to induce apoptosis and its sugar composition of EPS, further research is required to determine the probiotic mechanisms of action by which L. gasseri strains result in strain specific anti-proliferative activity.
28554813	1	15	theme	host	232:235	arg1	interaction					248:258	host microbiota interaction	232:258	host microbiota interaction	232:258	Lactobacilli, commonly used as probiotics, have been shown to maintain vaginal health and contribute to host microbiota interaction.
28554813	0	16	theme	cervical	99:106	arg1	HeLa					121:124	HeLa	121:124	HeLa	121:124	Impact of Exopolysaccharides (EPSs) of Lactobacillus gasseri strains isolated from human vagina on cervical tumor cells (HeLa).
28554813	0	16	theme	cervical	99:106	arg1	cells					114:118	cervical tumor cells	99:118	cervical tumor cells (HeLa)	99:125	Impact of Exopolysaccharides (EPSs) of Lactobacillus gasseri strains isolated from human vagina on cervical tumor cells (HeLa).
28554813	3	17	theme	cancer	659:664	arg1	growth					678:683	cervical cancer cell (HeLa) growth	650:683	cervical cancer cell (HeLa) growth	650:683	The objective of this study is to investigate and compare EPSs of L. gasseri strains (G10 and H15), isolated from a healthy human vagina, for their capability to inhibit cervical cancer cell (HeLa) growth and modulate immune response.
28554813	6	18	theme	relative	1138:1145	arg1	proportions					1147:1157	relative proportions	1138:1157	relative proportions of the individual monosaccharides except mannose	1138:1206	The sugar component was the same; however, relative proportions of the individual monosaccharides except mannose were different.
28554813	2	19	theme	probiotic	358:366	arg1	activity					368:375	probiotic activity	358:375	probiotic activity	358:375	Exopolysaccharides (EPSs) produced by lactobacillus have been found to have an important role in probiotic activity; however, there is limited knowledge concerning their impact on cervical cancer and urogenital health.
28554813	2	20	contain	have	332:335	arg1	EPSs					281:284	EPSs	281:284	EPSs	281:284	Exopolysaccharides (EPSs) produced by lactobacillus have been found to have an important role in probiotic activity; however, there is limited knowledge concerning their impact on cervical cancer and urogenital health.
28554813	2	20	contain	have	332:335	arg1	Exopolysaccharides					261:278	Exopolysaccharides	261:278	Exopolysaccharides (EPSs) produced by lactobacillus	261:311	Exopolysaccharides (EPSs) produced by lactobacillus have been found to have an important role in probiotic activity; however, there is limited knowledge concerning their impact on cervical cancer and urogenital health.
28554813	2	20	contain	have	332:335	arg2	role					350:353	an important role	337:353	an important role	337:353	Exopolysaccharides (EPSs) produced by lactobacillus have been found to have an important role in probiotic activity; however, there is limited knowledge concerning their impact on cervical cancer and urogenital health.
28554813	4	21	theme	host	905:908	arg1	cells					910:914	host cells	905:914	host cells	905:914	HeLa cells were treated with live culture at ∼108 CFU/ml or increasing concentration of lyophilized EPS (L-EPS) (100, 200, or 400 μg/ml) of L. gasseri strains and their ability to adhere to host cells, inhibit proliferation, and modulate immune response were evaluated.
28554813	2	22	from	impact	431:436	arg1	cancer					450:455	cervical cancer	441:455	cervical cancer	441:455	Exopolysaccharides (EPSs) produced by lactobacillus have been found to have an important role in probiotic activity; however, there is limited knowledge concerning their impact on cervical cancer and urogenital health.
28554813	2	22	from	impact	431:436	arg1	health					472:477	urogenital health	461:477	urogenital health	461:477	Exopolysaccharides (EPSs) produced by lactobacillus have been found to have an important role in probiotic activity; however, there is limited knowledge concerning their impact on cervical cancer and urogenital health.
28554813	3	23	theme	HeLa	672:675	arg1	growth					678:683	cervical cancer cell (HeLa) growth	650:683	cervical cancer cell (HeLa) growth	650:683	The objective of this study is to investigate and compare EPSs of L. gasseri strains (G10 and H15), isolated from a healthy human vagina, for their capability to inhibit cervical cancer cell (HeLa) growth and modulate immune response.
28554813	13	24	theme	action	2101:2106	arg1	mechanisms					2087:2096	the probiotic mechanisms	2073:2096	the probiotic mechanisms of action by which L. gasseri strains result in strain specific anti-proliferative activity	2073:2188	Although our results suggest a relationship between the ability of a strain to induce apoptosis and its sugar composition of EPS, further research is required to determine the probiotic mechanisms of action by which L. gasseri strains result in strain specific anti-proliferative activity.
28554813	9	25	from	apoptosis	1499:1507	arg1	cells					1517:1521	HeLa cells	1512:1521	HeLa cells	1512:1521	L-EPSs of L. gasseri strains induced apoptosis in HeLa cells in a strain dependent manner.
28554813	2	26	theme	limited	396:402	arg1	knowledge					404:412	limited knowledge	396:412	limited knowledge concerning their impact on cervical cancer and urogenital health	396:477	Exopolysaccharides (EPSs) produced by lactobacillus have been found to have an important role in probiotic activity; however, there is limited knowledge concerning their impact on cervical cancer and urogenital health.
28554813	1	27	theme	microbiota	237:246	arg1	interaction					248:258	host microbiota interaction	232:258	host microbiota interaction	232:258	Lactobacilli, commonly used as probiotics, have been shown to maintain vaginal health and contribute to host microbiota interaction.
28554813	12	28	from	diversity	1804:1812	arg1	composition					1823:1833	sugar composition	1817:1833	sugar composition of EPS	1817:1840	In conclusion, diversity in sugar composition of EPS might contribute to adhesion and proliferation properties.
28554813	9	29	theme	dependent	1535:1543	arg1	manner					1545:1550	a strain dependent manner	1526:1550	a strain dependent manner	1526:1550	L-EPSs of L. gasseri strains induced apoptosis in HeLa cells in a strain dependent manner.
28554813	2	30	theme	important	340:348	arg1	role					350:353	an important role	337:353	an important role	337:353	Exopolysaccharides (EPSs) produced by lactobacillus have been found to have an important role in probiotic activity; however, there is limited knowledge concerning their impact on cervical cancer and urogenital health.
28554813	11	31	from	impact	1693:1698	arg1	cells					1708:1712	HeLa cells	1703:1712	HeLa cells	1703:1712	L. gasseri strains showed an anti-inflammatory impact on HeLa cells by decreasing the production of TNF-α and increasing the IL-10 production.
28554813	9	32	theme	strains	1483:1489	arg1	L-EPSs					1462:1467	L-EPSs	1462:1467	L-EPSs of L. gasseri strains	1462:1489	L-EPSs of L. gasseri strains induced apoptosis in HeLa cells in a strain dependent manner.
28554813	0	33	dep	strains	61:67	arg1	isolated					69:76	isolated	69:76	strains isolated from human vagina on cervical tumor cells (HeLa)	61:125	Impact of Exopolysaccharides (EPSs) of Lactobacillus gasseri strains isolated from human vagina on cervical tumor cells (HeLa).
28554813	0	34	theme	Exopolysaccharides	10:27	arg1	Impact					0:5	Impact	0:5	Impact of Exopolysaccharides (EPSs) of Lactobacillus gasseri	0:59	Impact of Exopolysaccharides (EPSs) of Lactobacillus gasseri strains isolated from human vagina on cervical tumor cells (HeLa).
28554813	3	35	theme	cell	666:669	arg1	growth					678:683	cervical cancer cell (HeLa) growth	650:683	cervical cancer cell (HeLa) growth	650:683	The objective of this study is to investigate and compare EPSs of L. gasseri strains (G10 and H15), isolated from a healthy human vagina, for their capability to inhibit cervical cancer cell (HeLa) growth and modulate immune response.
28554813	0	36	theme	tumor	108:112	arg1	HeLa					121:124	HeLa	121:124	HeLa	121:124	Impact of Exopolysaccharides (EPSs) of Lactobacillus gasseri strains isolated from human vagina on cervical tumor cells (HeLa).
28554813	0	36	theme	tumor	108:112	arg1	cells					114:118	cervical tumor cells	99:118	cervical tumor cells (HeLa)	99:125	Impact of Exopolysaccharides (EPSs) of Lactobacillus gasseri strains isolated from human vagina on cervical tumor cells (HeLa).
28554813	7	37	theme	adhesive	1283:1290	arg1	strain					1292:1297	the most adhesive strain	1274:1297	the most adhesive strain	1274:1297	Although they both produce similar amount of EPS, the most adhesive strain was G10.
28554813	7	37	theme	adhesive	1283:1290	arg1	G10					1303:1305	G10	1303:1305	G10	1303:1305	Although they both produce similar amount of EPS, the most adhesive strain was G10.
28554813	4	38	theme	L. gasseri	855:864	arg1	strains					866:872	L. gasseri strains	855:872	L. gasseri strains	855:872	HeLa cells were treated with live culture at ∼108 CFU/ml or increasing concentration of lyophilized EPS (L-EPS) (100, 200, or 400 μg/ml) of L. gasseri strains and their ability to adhere to host cells, inhibit proliferation, and modulate immune response were evaluated.
28554813	4	39	theme	ability	884:890	arg1	L-EPS					820:824	L-EPS	820:824	L-EPS	820:824	HeLa cells were treated with live culture at ∼108 CFU/ml or increasing concentration of lyophilized EPS (L-EPS) (100, 200, or 400 μg/ml) of L. gasseri strains and their ability to adhere to host cells, inhibit proliferation, and modulate immune response were evaluated.
28554813	4	39	theme	ability	884:890	arg1	concentration					786:798	increasing concentration	775:798	increasing concentration of lyophilized EPS (L-EPS) (100, 200, or 400 μg/ml) of L. gasseri strains and their ability to adhere to host cells, inhibit proliferation, and modulate immune response	775:967	HeLa cells were treated with live culture at ∼108 CFU/ml or increasing concentration of lyophilized EPS (L-EPS) (100, 200, or 400 μg/ml) of L. gasseri strains and their ability to adhere to host cells, inhibit proliferation, and modulate immune response were evaluated.
28554813	4	39	theme	ability	884:890	arg1	400 μg/ml					841:849	100, 200, or 400 μg/ml	828:849	100, 200, or 400 μg/ml	828:849	HeLa cells were treated with live culture at ∼108 CFU/ml or increasing concentration of lyophilized EPS (L-EPS) (100, 200, or 400 μg/ml) of L. gasseri strains and their ability to adhere to host cells, inhibit proliferation, and modulate immune response were evaluated.
28554813	8	40	dep	strains	1342:1348	arg1	Both					1308:1311	Both	1308:1311	Both	1308:1311	Both live and L-EPS of L. gasseri strains were capable of inhibiting the cell proliferation of HeLa cells with the impact of L-EPS being strain specific.
28554813	8	40	dep	strains	1342:1348	arg1	L-EPS					1322:1326	L-EPS	1322:1326	L-EPS of L. gasseri	1322:1340	Both live and L-EPS of L. gasseri strains were capable of inhibiting the cell proliferation of HeLa cells with the impact of L-EPS being strain specific.
28554813	12	41	dep	adhesion	1862:1869	arg1	properties					1889:1898	properties	1889:1898	properties	1889:1898	In conclusion, diversity in sugar composition of EPS might contribute to adhesion and proliferation properties.
28554813	3	42	theme	L. gasseri	546:555	arg1	G10					566:568	G10	566:568	G10	566:568	The objective of this study is to investigate and compare EPSs of L. gasseri strains (G10 and H15), isolated from a healthy human vagina, for their capability to inhibit cervical cancer cell (HeLa) growth and modulate immune response.
28554813	3	42	theme	L. gasseri	546:555	arg1	strains					557:563	L. gasseri strains	546:563	L. gasseri strains (G10 and H15)	546:577	The objective of this study is to investigate and compare EPSs of L. gasseri strains (G10 and H15), isolated from a healthy human vagina, for their capability to inhibit cervical cancer cell (HeLa) growth and modulate immune response.
28554813	3	42	theme	L. gasseri	546:555	arg1	H15					574:576	H15	574:576	H15	574:576	The objective of this study is to investigate and compare EPSs of L. gasseri strains (G10 and H15), isolated from a healthy human vagina, for their capability to inhibit cervical cancer cell (HeLa) growth and modulate immune response.
28554813	3	43	dep	strains	557:563	arg1	G10					566:568	G10	566:568	G10	566:568	The objective of this study is to investigate and compare EPSs of L. gasseri strains (G10 and H15), isolated from a healthy human vagina, for their capability to inhibit cervical cancer cell (HeLa) growth and modulate immune response.
28554813	3	43	dep	strains	557:563	arg1	strains					557:563	L. gasseri strains	546:563	L. gasseri strains (G10 and H15)	546:577	The objective of this study is to investigate and compare EPSs of L. gasseri strains (G10 and H15), isolated from a healthy human vagina, for their capability to inhibit cervical cancer cell (HeLa) growth and modulate immune response.
28554813	3	43	dep	strains	557:563	arg1	H15					574:576	H15	574:576	H15	574:576	The objective of this study is to investigate and compare EPSs of L. gasseri strains (G10 and H15), isolated from a healthy human vagina, for their capability to inhibit cervical cancer cell (HeLa) growth and modulate immune response.
28554813	13	44	theme	strain	1970:1975	arg1	ability					1957:1963	the ability	1953:1963	the ability of a strain to induce apoptosis and its sugar composition of EPS	1953:2028	Although our results suggest a relationship between the ability of a strain to induce apoptosis and its sugar composition of EPS, further research is required to determine the probiotic mechanisms of action by which L. gasseri strains result in strain specific anti-proliferative activity.
28554813	3	45	attach	isolated	580:587	arg2	H15					574:576	H15	574:576	H15	574:576	The objective of this study is to investigate and compare EPSs of L. gasseri strains (G10 and H15), isolated from a healthy human vagina, for their capability to inhibit cervical cancer cell (HeLa) growth and modulate immune response.
28554813	3	45	attach	isolated	580:587	arg2	G10					566:568	G10	566:568	G10	566:568	The objective of this study is to investigate and compare EPSs of L. gasseri strains (G10 and H15), isolated from a healthy human vagina, for their capability to inhibit cervical cancer cell (HeLa) growth and modulate immune response.
28554813	3	45	attach	isolated	580:587	arg2	strains					557:563	L. gasseri strains	546:563	L. gasseri strains (G10 and H15)	546:577	The objective of this study is to investigate and compare EPSs of L. gasseri strains (G10 and H15), isolated from a healthy human vagina, for their capability to inhibit cervical cancer cell (HeLa) growth and modulate immune response.
28554813	3	45	attach	isolated	580:587	arg1	vagina					610:615	a healthy human vagina	594:615	a healthy human vagina	594:615	The objective of this study is to investigate and compare EPSs of L. gasseri strains (G10 and H15), isolated from a healthy human vagina, for their capability to inhibit cervical cancer cell (HeLa) growth and modulate immune response.
28554813	5	46	theme	L-EPSs	1033:1038	arg1	composition					1014:1024	monosaccharide composition	999:1024	monosaccharide composition of the L-EPSs produced by L. gasseri strains	999:1069	Additionally, monosaccharide composition of the L-EPSs produced by L. gasseri strains was determined by HPLC.
28554813	8	47	theme	L. gasseri	1331:1340	arg1	L-EPS					1322:1326	L-EPS	1322:1326	L-EPS of L. gasseri	1322:1340	Both live and L-EPS of L. gasseri strains were capable of inhibiting the cell proliferation of HeLa cells with the impact of L-EPS being strain specific.
28554813	3	48	theme	strains	557:563	arg1	EPSs					538:541	EPSs	538:541	EPSs of L. gasseri strains (G10 and H15), isolated from a healthy human vagina, for their capability to inhibit cervical cancer cell (HeLa) growth and modulate immune response	538:712	The objective of this study is to investigate and compare EPSs of L. gasseri strains (G10 and H15), isolated from a healthy human vagina, for their capability to inhibit cervical cancer cell (HeLa) growth and modulate immune response.
28554813	13	49	theme	probiotic	2077:2085	arg1	mechanisms					2087:2096	the probiotic mechanisms	2073:2096	the probiotic mechanisms of action by which L. gasseri strains result in strain specific anti-proliferative activity	2073:2188	Although our results suggest a relationship between the ability of a strain to induce apoptosis and its sugar composition of EPS, further research is required to determine the probiotic mechanisms of action by which L. gasseri strains result in strain specific anti-proliferative activity.
28554813	3	50	theme	immune	698:703	arg1	response					705:712	immune response	698:712	immune response	698:712	The objective of this study is to investigate and compare EPSs of L. gasseri strains (G10 and H15), isolated from a healthy human vagina, for their capability to inhibit cervical cancer cell (HeLa) growth and modulate immune response.
28554813	13	51	theme	sugar	2005:2009	arg1	composition					2011:2021	its sugar composition	2001:2021	its sugar composition	2001:2021	Although our results suggest a relationship between the ability of a strain to induce apoptosis and its sugar composition of EPS, further research is required to determine the probiotic mechanisms of action by which L. gasseri strains result in strain specific anti-proliferative activity.
28554813	4	52	theme	live	744:747	arg1	culture					749:755	live culture	744:755	live culture	744:755	HeLa cells were treated with live culture at ∼108 CFU/ml or increasing concentration of lyophilized EPS (L-EPS) (100, 200, or 400 μg/ml) of L. gasseri strains and their ability to adhere to host cells, inhibit proliferation, and modulate immune response were evaluated.
28554813	12	53	theme	sugar	1817:1821	arg1	composition					1823:1833	sugar composition	1817:1833	sugar composition of EPS	1817:1840	In conclusion, diversity in sugar composition of EPS might contribute to adhesion and proliferation properties.
28554813	11	54	theme	anti-inflammatory	1675:1691	arg1	impact					1693:1698	an anti-inflammatory impact	1672:1698	an anti-inflammatory impact on HeLa cells	1672:1712	L. gasseri strains showed an anti-inflammatory impact on HeLa cells by decreasing the production of TNF-α and increasing the IL-10 production.
28554813	4	55	theme	EPS	815:817	arg1	L-EPS					820:824	L-EPS	820:824	L-EPS	820:824	HeLa cells were treated with live culture at ∼108 CFU/ml or increasing concentration of lyophilized EPS (L-EPS) (100, 200, or 400 μg/ml) of L. gasseri strains and their ability to adhere to host cells, inhibit proliferation, and modulate immune response were evaluated.
28554813	4	55	theme	EPS	815:817	arg1	concentration					786:798	increasing concentration	775:798	increasing concentration of lyophilized EPS (L-EPS) (100, 200, or 400 μg/ml) of L. gasseri strains and their ability to adhere to host cells, inhibit proliferation, and modulate immune response	775:967	HeLa cells were treated with live culture at ∼108 CFU/ml or increasing concentration of lyophilized EPS (L-EPS) (100, 200, or 400 μg/ml) of L. gasseri strains and their ability to adhere to host cells, inhibit proliferation, and modulate immune response were evaluated.
28554813	4	55	theme	EPS	815:817	arg1	400 μg/ml					841:849	100, 200, or 400 μg/ml	828:849	100, 200, or 400 μg/ml	828:849	HeLa cells were treated with live culture at ∼108 CFU/ml or increasing concentration of lyophilized EPS (L-EPS) (100, 200, or 400 μg/ml) of L. gasseri strains and their ability to adhere to host cells, inhibit proliferation, and modulate immune response were evaluated.
28554813	3	56	theme	human	604:608	arg1	vagina					610:615	a healthy human vagina	594:615	a healthy human vagina	594:615	The objective of this study is to investigate and compare EPSs of L. gasseri strains (G10 and H15), isolated from a healthy human vagina, for their capability to inhibit cervical cancer cell (HeLa) growth and modulate immune response.
28554813	7	57	theme	EPS	1269:1271	arg1	EPS					1269:1271	EPS	1269:1271	EPS	1269:1271	Although they both produce similar amount of EPS, the most adhesive strain was G10.
28554813	7	57	theme	EPS	1269:1271	arg1	amount					1259:1264	similar amount	1251:1264	similar amount of EPS	1251:1271	Although they both produce similar amount of EPS, the most adhesive strain was G10.
28554813	8	58	dep	Both	1308:1311	arg1	live					1313:1316	live	1313:1316	live	1313:1316	Both live and L-EPS of L. gasseri strains were capable of inhibiting the cell proliferation of HeLa cells with the impact of L-EPS being strain specific.
28554813	4	59	theme	immune	953:958	arg1	response					960:967	immune response	953:967	immune response	953:967	HeLa cells were treated with live culture at ∼108 CFU/ml or increasing concentration of lyophilized EPS (L-EPS) (100, 200, or 400 μg/ml) of L. gasseri strains and their ability to adhere to host cells, inhibit proliferation, and modulate immune response were evaluated.
28554813	5	60	theme	L. gasseri	1052:1061	arg1	strains					1063:1069	L. gasseri strains	1052:1069	L. gasseri strains	1052:1069	Additionally, monosaccharide composition of the L-EPSs produced by L. gasseri strains was determined by HPLC.
28554813	7	61	theme	similar	1251:1257	arg1	EPS					1269:1271	EPS	1269:1271	EPS	1269:1271	Although they both produce similar amount of EPS, the most adhesive strain was G10.
28554813	7	61	theme	similar	1251:1257	arg1	amount					1259:1264	similar amount	1251:1264	similar amount of EPS	1251:1271	Although they both produce similar amount of EPS, the most adhesive strain was G10.
28554813	6	62	theme	sugar	1099:1103	arg1	component					1105:1113	The sugar component	1095:1113	The sugar component	1095:1113	The sugar component was the same; however, relative proportions of the individual monosaccharides except mannose were different.
28554813	6	62	theme	sugar	1099:1103	arg1	same					1123:1126	same	1123:1126	same	1123:1126	The sugar component was the same; however, relative proportions of the individual monosaccharides except mannose were different.
28554813	9	63	theme	HeLa	1512:1515	arg1	cells					1517:1521	HeLa cells	1512:1521	HeLa cells	1512:1521	L-EPSs of L. gasseri strains induced apoptosis in HeLa cells in a strain dependent manner.
28554813	13	64	theme	specific	2153:2160	arg1	activity					2181:2188	strain specific anti-proliferative activity	2146:2188	strain specific anti-proliferative activity	2146:2188	Although our results suggest a relationship between the ability of a strain to induce apoptosis and its sugar composition of EPS, further research is required to determine the probiotic mechanisms of action by which L. gasseri strains result in strain specific anti-proliferative activity.
28554813	10	65	theme	Caspase	1635:1641	arg1	upregulation					1611:1622	an upregulation	1608:1622	an upregulation of Bax and Caspase 3	1608:1643	The ability to induce apoptosis by G10 associated with an upregulation of Bax and Caspase 3.
28554813	13	66	theme	further	2031:2037	arg1	research					2039:2046	further research	2031:2046	further research	2031:2046	Although our results suggest a relationship between the ability of a strain to induce apoptosis and its sugar composition of EPS, further research is required to determine the probiotic mechanisms of action by which L. gasseri strains result in strain specific anti-proliferative activity.
28554813	6	67	theme	monosaccharides	1177:1191	arg1	proportions					1147:1157	relative proportions	1138:1157	relative proportions of the individual monosaccharides except mannose	1138:1206	The sugar component was the same; however, relative proportions of the individual monosaccharides except mannose were different.
28554813	0	68	from	vagina	89:94	arg1	HeLa					121:124	HeLa	121:124	HeLa	121:124	Impact of Exopolysaccharides (EPSs) of Lactobacillus gasseri strains isolated from human vagina on cervical tumor cells (HeLa).
28554813	0	68	from	vagina	89:94	arg1	cells					114:118	cervical tumor cells	99:118	cervical tumor cells (HeLa)	99:125	Impact of Exopolysaccharides (EPSs) of Lactobacillus gasseri strains isolated from human vagina on cervical tumor cells (HeLa).
28554813	10	69	theme	Bax	1627:1629	arg1	upregulation					1611:1622	an upregulation	1608:1622	an upregulation of Bax and Caspase 3	1608:1643	The ability to induce apoptosis by G10 associated with an upregulation of Bax and Caspase 3.
28554813	13	70	theme	L. gasseri	2117:2126	arg1	strains					2128:2134	L. gasseri strains	2117:2134	L. gasseri strains	2117:2134	Although our results suggest a relationship between the ability of a strain to induce apoptosis and its sugar composition of EPS, further research is required to determine the probiotic mechanisms of action by which L. gasseri strains result in strain specific anti-proliferative activity.
28554813	13	71	theme	anti-proliferative	2162:2179	arg1	activity					2181:2188	strain specific anti-proliferative activity	2146:2188	strain specific anti-proliferative activity	2146:2188	Although our results suggest a relationship between the ability of a strain to induce apoptosis and its sugar composition of EPS, further research is required to determine the probiotic mechanisms of action by which L. gasseri strains result in strain specific anti-proliferative activity.
28554813	2	72	theme	urogenital	461:470	arg1	health					472:477	urogenital health	461:477	urogenital health	461:477	Exopolysaccharides (EPSs) produced by lactobacillus have been found to have an important role in probiotic activity; however, there is limited knowledge concerning their impact on cervical cancer and urogenital health.
28554813	3	73	theme	healthy	596:602	arg1	vagina					610:615	a healthy human vagina	594:615	a healthy human vagina	594:615	The objective of this study is to investigate and compare EPSs of L. gasseri strains (G10 and H15), isolated from a healthy human vagina, for their capability to inhibit cervical cancer cell (HeLa) growth and modulate immune response.
28554813	11	74	theme	TNF-α	1746:1750	arg1	production					1732:1741	the production	1728:1741	the production of TNF-α	1728:1750	L. gasseri strains showed an anti-inflammatory impact on HeLa cells by decreasing the production of TNF-α and increasing the IL-10 production.
28554813	11	75	theme	IL-10	1771:1775	arg1	production					1777:1786	the IL-10 production	1767:1786	the IL-10 production	1767:1786	L. gasseri strains showed an anti-inflammatory impact on HeLa cells by decreasing the production of TNF-α and increasing the IL-10 production.
28554813	1	76	theme	vaginal	199:205	arg1	health					207:212	vaginal health	199:212	vaginal health	199:212	Lactobacilli, commonly used as probiotics, have been shown to maintain vaginal health and contribute to host microbiota interaction.
28554813	0	77	theme	gasseri	53:59	arg1	Impact					0:5	Impact	0:5	Impact of Exopolysaccharides (EPSs) of Lactobacillus gasseri	0:59	Impact of Exopolysaccharides (EPSs) of Lactobacillus gasseri strains isolated from human vagina on cervical tumor cells (HeLa).
28554813	5	78	theme	monosaccharide	999:1012	arg1	composition					1014:1024	monosaccharide composition	999:1024	monosaccharide composition of the L-EPSs produced by L. gasseri strains	999:1069	Additionally, monosaccharide composition of the L-EPSs produced by L. gasseri strains was determined by HPLC.
28554813	4	79	theme	HeLa	715:718	arg1	cells					720:724	HeLa cells	715:724	HeLa cells	715:724	HeLa cells were treated with live culture at ∼108 CFU/ml or increasing concentration of lyophilized EPS (L-EPS) (100, 200, or 400 μg/ml) of L. gasseri strains and their ability to adhere to host cells, inhibit proliferation, and modulate immune response were evaluated.
28554813	12	80	theme	EPS	1838:1840	arg1	composition					1823:1833	sugar composition	1817:1833	sugar composition of EPS	1817:1840	In conclusion, diversity in sugar composition of EPS might contribute to adhesion and proliferation properties.
28554813	13	81	theme	EPS	2026:2028	arg1	composition					2011:2021	its sugar composition	2001:2021	its sugar composition	2001:2021	Although our results suggest a relationship between the ability of a strain to induce apoptosis and its sugar composition of EPS, further research is required to determine the probiotic mechanisms of action by which L. gasseri strains result in strain specific anti-proliferative activity.
28554813	13	81	theme	EPS	2026:2028	arg1	apoptosis					1987:1995	apoptosis	1987:1995	apoptosis	1987:1995	Although our results suggest a relationship between the ability of a strain to induce apoptosis and its sugar composition of EPS, further research is required to determine the probiotic mechanisms of action by which L. gasseri strains result in strain specific anti-proliferative activity.
28554813	11	82	theme	HeLa	1703:1706	arg1	cells					1708:1712	HeLa cells	1703:1712	HeLa cells	1703:1712	L. gasseri strains showed an anti-inflammatory impact on HeLa cells by decreasing the production of TNF-α and increasing the IL-10 production.
29240705	1	0	theme	diffraction	379:389	arg1	methods					424:430	X-ray diffraction and scanning electron microscopy methods	373:430	X-ray diffraction and scanning electron microscopy methods	373:430	Two-ply composite membranes with separation layers from chitosan and sulfoethylcellulose were developed on a microporous support based on poly(diphenylsulfone-N-phenylphthalimide) and investigated by use of X-ray diffraction and scanning electron microscopy methods.
29240705	2	1	dep	alcohol	522:528	arg1	propan-2-ol					540:550	propan-2-ol	540:550	propan-2-ol	540:550	The pervaporation properties of the membranes were studied for the separation of aqueous alcohol (ethanol, propan-2-ol) mixtures of different compositions.
29240705	2	1	dep	alcohol	522:528	arg1	ethanol					531:537	ethanol	531:537	ethanol	531:537	The pervaporation properties of the membranes were studied for the separation of aqueous alcohol (ethanol, propan-2-ol) mixtures of different compositions.
29240705	5	2	theme	significant	1135:1145	arg1	prospects					1147:1155	significant prospects	1135:1155	significant prospects for the use of chitosan and sulfoethylcellulose for the formation of highly selective pervaporation membranes	1135:1265	The results indicate significant prospects for the use of chitosan and sulfoethylcellulose for the formation of highly selective pervaporation membranes.
29240705	1	3	theme	composite	174:182	arg1	membranes					184:192	Two-ply composite membranes	166:192	Two-ply composite membranes with separation layers from chitosan and sulfoethylcellulose	166:253	Two-ply composite membranes with separation layers from chitosan and sulfoethylcellulose were developed on a microporous support based on poly(diphenylsulfone-N-phenylphthalimide) and investigated by use of X-ray diffraction and scanning electron microscopy methods.
29240705	0	4	from	Chitosan	56:63	arg1	Membranes					18:26	Two-Ply Composite Membranes	0:26	Two-Ply Composite Membranes with Separation Layers from Chitosan and Sulfoethylcellulose on a Microporous Support Based on Poly(diphenylsulfone-N-phenylphthalimide).	0:164	Two-Ply Composite Membranes with Separation Layers from Chitosan and Sulfoethylcellulose on a Microporous Support Based on Poly(diphenylsulfone-N-phenylphthalimide).
29240705	0	4	from	Chitosan	56:63	arg1	Support					106:112	a Microporous Support	92:112	a Microporous Support Based on Poly(diphenylsulfone-N-phenylphthalimide)	92:163	Two-Ply Composite Membranes with Separation Layers from Chitosan and Sulfoethylcellulose on a Microporous Support Based on Poly(diphenylsulfone-N-phenylphthalimide).
29240705	4	5	theme	non-porous	930:939	arg1	layer					941:945	a non-porous layer	928:945	a non-porous layer of the polyelectrolyte complex formed on the substrate surface	928:1008	Factors affecting the structure of a non-porous layer of the polyelectrolyte complex formed on the substrate surface and the contribution of that complex to changes in the transport properties of membranes are discussed.
29240705	3	6	theme	same	726:729	arg1	fraction					737:744	the same molar fraction	722:744	the same molar fraction of ionogenic groups (-NH₃⁺ for chitosan and -SO₃- for sulfoethylcellulose)	722:819	When the mixtures to be separated consist of less than 15 wt % water in propan-2-ol, the membranes composed of polyelectrolytes with the same molar fraction of ionogenic groups (-NH₃⁺ for chitosan and -SO₃- for sulfoethylcellulose) show high permselectivity (the water content in the permeate was 100%).
29240705	1	7	with	membranes	184:192	arg1	layers					210:215	separation layers	199:215	separation layers from chitosan and sulfoethylcellulose	199:253	Two-ply composite membranes with separation layers from chitosan and sulfoethylcellulose were developed on a microporous support based on poly(diphenylsulfone-N-phenylphthalimide) and investigated by use of X-ray diffraction and scanning electron microscopy methods.
29240705	5	8	theme	selective	1233:1241	arg1	membranes					1257:1265	highly selective pervaporation membranes	1226:1265	highly selective pervaporation membranes	1226:1265	The results indicate significant prospects for the use of chitosan and sulfoethylcellulose for the formation of highly selective pervaporation membranes.
29240705	4	9	theme	complex	970:976	arg1	layer					941:945	a non-porous layer	928:945	a non-porous layer of the polyelectrolyte complex formed on the substrate surface	928:1008	Factors affecting the structure of a non-porous layer of the polyelectrolyte complex formed on the substrate surface and the contribution of that complex to changes in the transport properties of membranes are discussed.
29240705	1	10	theme	scanning	395:402	arg1	microscopy					413:422	scanning electron microscopy	395:422	scanning electron microscopy	395:422	Two-ply composite membranes with separation layers from chitosan and sulfoethylcellulose were developed on a microporous support based on poly(diphenylsulfone-N-phenylphthalimide) and investigated by use of X-ray diffraction and scanning electron microscopy methods.
29240705	3	11	theme	water	852:856	arg1	content					858:864	the water content	848:864	the water content in the permeate	848:880	When the mixtures to be separated consist of less than 15 wt % water in propan-2-ol, the membranes composed of polyelectrolytes with the same molar fraction of ionogenic groups (-NH₃⁺ for chitosan and -SO₃- for sulfoethylcellulose) show high permselectivity (the water content in the permeate was 100%).
29240705	3	11	theme	water	852:856	arg1	%					889:889	100%	886:889	100%	886:889	When the mixtures to be separated consist of less than 15 wt % water in propan-2-ol, the membranes composed of polyelectrolytes with the same molar fraction of ionogenic groups (-NH₃⁺ for chitosan and -SO₃- for sulfoethylcellulose) show high permselectivity (the water content in the permeate was 100%).
29240705	0	12	theme	Microporous	94:104	arg1	Support					106:112	a Microporous Support	92:112	a Microporous Support Based on Poly(diphenylsulfone-N-phenylphthalimide)	92:163	Two-Ply Composite Membranes with Separation Layers from Chitosan and Sulfoethylcellulose on a Microporous Support Based on Poly(diphenylsulfone-N-phenylphthalimide).
29240705	1	13	theme	electron	404:411	arg1	microscopy					413:422	scanning electron microscopy	395:422	scanning electron microscopy	395:422	Two-ply composite membranes with separation layers from chitosan and sulfoethylcellulose were developed on a microporous support based on poly(diphenylsulfone-N-phenylphthalimide) and investigated by use of X-ray diffraction and scanning electron microscopy methods.
29240705	5	14	theme	chitosan	1172:1179	arg1	use					1165:1167	the use	1161:1167	the use of chitosan and sulfoethylcellulose	1161:1203	The results indicate significant prospects for the use of chitosan and sulfoethylcellulose for the formation of highly selective pervaporation membranes.
29240705	5	15	theme	membranes	1257:1265	arg1	formation					1213:1221	the formation	1209:1221	the formation of highly selective pervaporation membranes	1209:1265	The results indicate significant prospects for the use of chitosan and sulfoethylcellulose for the formation of highly selective pervaporation membranes.
29240705	2	16	theme	different	565:573	arg1	compositions					575:586	different compositions	565:586	different compositions	565:586	The pervaporation properties of the membranes were studied for the separation of aqueous alcohol (ethanol, propan-2-ol) mixtures of different compositions.
29240705	1	17	theme	microscopy	413:422	arg1	methods					424:430	X-ray diffraction and scanning electron microscopy methods	373:430	X-ray diffraction and scanning electron microscopy methods	373:430	Two-ply composite membranes with separation layers from chitosan and sulfoethylcellulose were developed on a microporous support based on poly(diphenylsulfone-N-phenylphthalimide) and investigated by use of X-ray diffraction and scanning electron microscopy methods.
29240705	3	18	from	water	652:656	arg1	propan-2-ol					661:671	propan-2-ol	661:671	propan-2-ol	661:671	When the mixtures to be separated consist of less than 15 wt % water in propan-2-ol, the membranes composed of polyelectrolytes with the same molar fraction of ionogenic groups (-NH₃⁺ for chitosan and -SO₃- for sulfoethylcellulose) show high permselectivity (the water content in the permeate was 100%).
29240705	1	19	theme	methods	424:430	arg1	use					366:368	use	366:368	use of X-ray diffraction and scanning electron microscopy methods	366:430	Two-ply composite membranes with separation layers from chitosan and sulfoethylcellulose were developed on a microporous support based on poly(diphenylsulfone-N-phenylphthalimide) and investigated by use of X-ray diffraction and scanning electron microscopy methods.
29240705	4	20	theme	complex	1039:1045	arg1	Factors					893:899	Factors	893:899	Factors affecting the structure of a non-porous layer of the polyelectrolyte complex formed on the substrate surface	893:1008	Factors affecting the structure of a non-porous layer of the polyelectrolyte complex formed on the substrate surface and the contribution of that complex to changes in the transport properties of membranes are discussed.
29240705	4	20	theme	complex	1039:1045	arg1	contribution					1018:1029	the contribution	1014:1029	the contribution of that complex to changes in the transport properties of membranes	1014:1097	Factors affecting the structure of a non-porous layer of the polyelectrolyte complex formed on the substrate surface and the contribution of that complex to changes in the transport properties of membranes are discussed.
29240705	1	21	theme	separation	199:208	arg1	layers					210:215	separation layers	199:215	separation layers from chitosan and sulfoethylcellulose	199:253	Two-ply composite membranes with separation layers from chitosan and sulfoethylcellulose were developed on a microporous support based on poly(diphenylsulfone-N-phenylphthalimide) and investigated by use of X-ray diffraction and scanning electron microscopy methods.
29240705	0	22	theme	Composite	8:16	arg1	Membranes					18:26	Two-Ply Composite Membranes	0:26	Two-Ply Composite Membranes with Separation Layers from Chitosan and Sulfoethylcellulose on a Microporous Support Based on Poly(diphenylsulfone-N-phenylphthalimide).	0:164	Two-Ply Composite Membranes with Separation Layers from Chitosan and Sulfoethylcellulose on a Microporous Support Based on Poly(diphenylsulfone-N-phenylphthalimide).
29240705	3	23	theme	groups	759:764	arg1	fraction					737:744	the same molar fraction	722:744	the same molar fraction of ionogenic groups (-NH₃⁺ for chitosan and -SO₃- for sulfoethylcellulose)	722:819	When the mixtures to be separated consist of less than 15 wt % water in propan-2-ol, the membranes composed of polyelectrolytes with the same molar fraction of ionogenic groups (-NH₃⁺ for chitosan and -SO₃- for sulfoethylcellulose) show high permselectivity (the water content in the permeate was 100%).
29240705	4	24	theme	substrate	992:1000	arg1	surface					1002:1008	the substrate surface	988:1008	the substrate surface	988:1008	Factors affecting the structure of a non-porous layer of the polyelectrolyte complex formed on the substrate surface and the contribution of that complex to changes in the transport properties of membranes are discussed.
29240705	2	25	theme	pervaporation	437:449	arg1	properties					451:460	The pervaporation properties	433:460	The pervaporation properties of the membranes	433:477	The pervaporation properties of the membranes were studied for the separation of aqueous alcohol (ethanol, propan-2-ol) mixtures of different compositions.
29240705	0	26	theme	Two-Ply	0:6	arg1	Membranes					18:26	Two-Ply Composite Membranes	0:26	Two-Ply Composite Membranes with Separation Layers from Chitosan and Sulfoethylcellulose on a Microporous Support Based on Poly(diphenylsulfone-N-phenylphthalimide).	0:164	Two-Ply Composite Membranes with Separation Layers from Chitosan and Sulfoethylcellulose on a Microporous Support Based on Poly(diphenylsulfone-N-phenylphthalimide).
29240705	3	27	theme	molar	731:735	arg1	fraction					737:744	the same molar fraction	722:744	the same molar fraction of ionogenic groups (-NH₃⁺ for chitosan and -SO₃- for sulfoethylcellulose)	722:819	When the mixtures to be separated consist of less than 15 wt % water in propan-2-ol, the membranes composed of polyelectrolytes with the same molar fraction of ionogenic groups (-NH₃⁺ for chitosan and -SO₃- for sulfoethylcellulose) show high permselectivity (the water content in the permeate was 100%).
29240705	4	28	theme	layer	941:945	arg1	structure					915:923	the structure	911:923	the structure of a non-porous layer of the polyelectrolyte complex formed on the substrate surface	911:1008	Factors affecting the structure of a non-porous layer of the polyelectrolyte complex formed on the substrate surface and the contribution of that complex to changes in the transport properties of membranes are discussed.
29240705	3	29	from	content	858:864	arg1	the					869:871	the	869:871	the	869:871	When the mixtures to be separated consist of less than 15 wt % water in propan-2-ol, the membranes composed of polyelectrolytes with the same molar fraction of ionogenic groups (-NH₃⁺ for chitosan and -SO₃- for sulfoethylcellulose) show high permselectivity (the water content in the permeate was 100%).
29240705	3	30	with	polyelectrolytes	700:715	arg1	fraction					737:744	the same molar fraction	722:744	the same molar fraction of ionogenic groups (-NH₃⁺ for chitosan and -SO₃- for sulfoethylcellulose)	722:819	When the mixtures to be separated consist of less than 15 wt % water in propan-2-ol, the membranes composed of polyelectrolytes with the same molar fraction of ionogenic groups (-NH₃⁺ for chitosan and -SO₃- for sulfoethylcellulose) show high permselectivity (the water content in the permeate was 100%).
29240705	4	31	theme	polyelectrolyte	954:968	arg1	complex					970:976	the polyelectrolyte complex	950:976	the polyelectrolyte complex formed on the substrate surface	950:1008	Factors affecting the structure of a non-porous layer of the polyelectrolyte complex formed on the substrate surface and the contribution of that complex to changes in the transport properties of membranes are discussed.
29240705	2	32	theme	membranes	469:477	arg1	properties					451:460	The pervaporation properties	433:460	The pervaporation properties of the membranes	433:477	The pervaporation properties of the membranes were studied for the separation of aqueous alcohol (ethanol, propan-2-ol) mixtures of different compositions.
29240705	0	33	theme	Separation	33:42	arg1	Layers					44:49	Separation Layers	33:49	Separation Layers	33:49	Two-Ply Composite Membranes with Separation Layers from Chitosan and Sulfoethylcellulose on a Microporous Support Based on Poly(diphenylsulfone-N-phenylphthalimide).
29240705	0	34	from	Sulfoethylcellulose	69:87	arg1	Membranes					18:26	Two-Ply Composite Membranes	0:26	Two-Ply Composite Membranes with Separation Layers from Chitosan and Sulfoethylcellulose on a Microporous Support Based on Poly(diphenylsulfone-N-phenylphthalimide).	0:164	Two-Ply Composite Membranes with Separation Layers from Chitosan and Sulfoethylcellulose on a Microporous Support Based on Poly(diphenylsulfone-N-phenylphthalimide).
29240705	0	34	from	Sulfoethylcellulose	69:87	arg1	Support					106:112	a Microporous Support	92:112	a Microporous Support Based on Poly(diphenylsulfone-N-phenylphthalimide)	92:163	Two-Ply Composite Membranes with Separation Layers from Chitosan and Sulfoethylcellulose on a Microporous Support Based on Poly(diphenylsulfone-N-phenylphthalimide).
29240705	3	35	theme	wt	647:648	arg1	water					652:656	less than 15 wt % water	634:656	less than 15 wt % water in propan-2-ol	634:671	When the mixtures to be separated consist of less than 15 wt % water in propan-2-ol, the membranes composed of polyelectrolytes with the same molar fraction of ionogenic groups (-NH₃⁺ for chitosan and -SO₃- for sulfoethylcellulose) show high permselectivity (the water content in the permeate was 100%).
29240705	1	36	from	sulfoethylcellulose	235:253	arg1	layers					210:215	separation layers	199:215	separation layers from chitosan and sulfoethylcellulose	199:253	Two-ply composite membranes with separation layers from chitosan and sulfoethylcellulose were developed on a microporous support based on poly(diphenylsulfone-N-phenylphthalimide) and investigated by use of X-ray diffraction and scanning electron microscopy methods.
29240705	2	37	theme	alcohol	522:528	arg1	mixtures					553:560	aqueous alcohol (ethanol, propan-2-ol) mixtures	514:560	aqueous alcohol (ethanol, propan-2-ol) mixtures of different compositions	514:586	The pervaporation properties of the membranes were studied for the separation of aqueous alcohol (ethanol, propan-2-ol) mixtures of different compositions.
29240705	3	38	dep	groups	759:764	arg1	-SO₃-					790:794	-SO₃-	790:794	-SO₃-	790:794	When the mixtures to be separated consist of less than 15 wt % water in propan-2-ol, the membranes composed of polyelectrolytes with the same molar fraction of ionogenic groups (-NH₃⁺ for chitosan and -SO₃- for sulfoethylcellulose) show high permselectivity (the water content in the permeate was 100%).
29240705	3	38	dep	groups	759:764	arg1	-NH₃⁺					767:771	-NH₃⁺	767:771	-NH₃⁺	767:771	When the mixtures to be separated consist of less than 15 wt % water in propan-2-ol, the membranes composed of polyelectrolytes with the same molar fraction of ionogenic groups (-NH₃⁺ for chitosan and -SO₃- for sulfoethylcellulose) show high permselectivity (the water content in the permeate was 100%).
29240705	4	39	from	changes	1050:1056	arg1	properties					1075:1084	the transport properties	1061:1084	the transport properties of membranes	1061:1097	Factors affecting the structure of a non-porous layer of the polyelectrolyte complex formed on the substrate surface and the contribution of that complex to changes in the transport properties of membranes are discussed.
29240705	2	40	theme	compositions	575:586	arg1	mixtures					553:560	aqueous alcohol (ethanol, propan-2-ol) mixtures	514:560	aqueous alcohol (ethanol, propan-2-ol) mixtures of different compositions	514:586	The pervaporation properties of the membranes were studied for the separation of aqueous alcohol (ethanol, propan-2-ol) mixtures of different compositions.
29240705	0	41	with	Membranes	18:26	arg1	Layers					44:49	Separation Layers	33:49	Separation Layers	33:49	Two-Ply Composite Membranes with Separation Layers from Chitosan and Sulfoethylcellulose on a Microporous Support Based on Poly(diphenylsulfone-N-phenylphthalimide).
29240705	2	42	theme	aqueous	514:520	arg1	alcohol					522:528	aqueous alcohol	514:528	aqueous alcohol (ethanol, propan-2-ol) mixtures of different compositions	514:586	The pervaporation properties of the membranes were studied for the separation of aqueous alcohol (ethanol, propan-2-ol) mixtures of different compositions.
29240705	3	43	theme	ionogenic	749:757	arg1	groups					759:764	ionogenic groups	749:764	ionogenic groups (-NH₃⁺ for chitosan and -SO₃- for sulfoethylcellulose)	749:819	When the mixtures to be separated consist of less than 15 wt % water in propan-2-ol, the membranes composed of polyelectrolytes with the same molar fraction of ionogenic groups (-NH₃⁺ for chitosan and -SO₃- for sulfoethylcellulose) show high permselectivity (the water content in the permeate was 100%).
29240705	1	44	from	chitosan	222:229	arg1	layers					210:215	separation layers	199:215	separation layers from chitosan and sulfoethylcellulose	199:253	Two-ply composite membranes with separation layers from chitosan and sulfoethylcellulose were developed on a microporous support based on poly(diphenylsulfone-N-phenylphthalimide) and investigated by use of X-ray diffraction and scanning electron microscopy methods.
29240705	5	45	theme	sulfoethylcellulose	1185:1203	arg1	use					1165:1167	the use	1161:1167	the use of chitosan and sulfoethylcellulose	1161:1203	The results indicate significant prospects for the use of chitosan and sulfoethylcellulose for the formation of highly selective pervaporation membranes.
29240705	3	46	dep	mixtures	598:605	arg1	separated					613:621	separated	613:621	to be separated	607:621	When the mixtures to be separated consist of less than 15 wt % water in propan-2-ol, the membranes composed of polyelectrolytes with the same molar fraction of ionogenic groups (-NH₃⁺ for chitosan and -SO₃- for sulfoethylcellulose) show high permselectivity (the water content in the permeate was 100%).
29240705	4	47	theme	membranes	1089:1097	arg1	properties					1075:1084	the transport properties	1061:1084	the transport properties of membranes	1061:1097	Factors affecting the structure of a non-porous layer of the polyelectrolyte complex formed on the substrate surface and the contribution of that complex to changes in the transport properties of membranes are discussed.
29240705	2	48	theme	mixtures	553:560	arg1	separation					500:509	the separation	496:509	the separation of aqueous alcohol (ethanol, propan-2-ol) mixtures of different compositions	496:586	The pervaporation properties of the membranes were studied for the separation of aqueous alcohol (ethanol, propan-2-ol) mixtures of different compositions.
29240705	3	49	dep	the	869:871	arg1	permeate					873:880	permeate	873:880	permeate	873:880	When the mixtures to be separated consist of less than 15 wt % water in propan-2-ol, the membranes composed of polyelectrolytes with the same molar fraction of ionogenic groups (-NH₃⁺ for chitosan and -SO₃- for sulfoethylcellulose) show high permselectivity (the water content in the permeate was 100%).
29240705	3	50	theme	%	650:650	arg1	water					652:656	less than 15 wt % water	634:656	less than 15 wt % water in propan-2-ol	634:671	When the mixtures to be separated consist of less than 15 wt % water in propan-2-ol, the membranes composed of polyelectrolytes with the same molar fraction of ionogenic groups (-NH₃⁺ for chitosan and -SO₃- for sulfoethylcellulose) show high permselectivity (the water content in the permeate was 100%).
29240705	3	51	theme	high	826:829	arg1	permselectivity					831:845	high permselectivity	826:845	high permselectivity	826:845	When the mixtures to be separated consist of less than 15 wt % water in propan-2-ol, the membranes composed of polyelectrolytes with the same molar fraction of ionogenic groups (-NH₃⁺ for chitosan and -SO₃- for sulfoethylcellulose) show high permselectivity (the water content in the permeate was 100%).
29240705	5	52	theme	pervaporation	1243:1255	arg1	membranes					1257:1265	highly selective pervaporation membranes	1226:1265	highly selective pervaporation membranes	1226:1265	The results indicate significant prospects for the use of chitosan and sulfoethylcellulose for the formation of highly selective pervaporation membranes.
29240705	1	53	theme	X-ray	373:377	arg1	diffraction					379:389	X-ray diffraction	373:389	X-ray diffraction	373:389	Two-ply composite membranes with separation layers from chitosan and sulfoethylcellulose were developed on a microporous support based on poly(diphenylsulfone-N-phenylphthalimide) and investigated by use of X-ray diffraction and scanning electron microscopy methods.
29240705	1	54	theme	Two-ply	166:172	arg1	membranes					184:192	Two-ply composite membranes	166:192	Two-ply composite membranes with separation layers from chitosan and sulfoethylcellulose	166:253	Two-ply composite membranes with separation layers from chitosan and sulfoethylcellulose were developed on a microporous support based on poly(diphenylsulfone-N-phenylphthalimide) and investigated by use of X-ray diffraction and scanning electron microscopy methods.
29240705	4	55	theme	transport	1065:1073	arg1	properties					1075:1084	the transport properties	1061:1084	the transport properties of membranes	1061:1097	Factors affecting the structure of a non-porous layer of the polyelectrolyte complex formed on the substrate surface and the contribution of that complex to changes in the transport properties of membranes are discussed.
29240705	1	56	theme	microporous	275:285	arg1	support					287:293	a microporous support	273:293	a microporous support based on poly(diphenylsulfone-N-phenylphthalimide)	273:344	Two-ply composite membranes with separation layers from chitosan and sulfoethylcellulose were developed on a microporous support based on poly(diphenylsulfone-N-phenylphthalimide) and investigated by use of X-ray diffraction and scanning electron microscopy methods.
28797225	11	0	theme	different	1990:1998	arg1	strains					2013:2019	different K. baliensis strains	1990:2019	different K. baliensis strains	1990:2019	A relatively high intrinsic acetic acid tolerance was also observable for K. baliensis DSM 14400T, which might indicate diverse adaptation mechanisms of different K. baliensis strains in altering natural habitats.
28797225	6	1	theme	polE	1081:1084	arg1	gene					1086:1089	the polE gene	1077:1089	the polE gene of the R strain	1077:1105	To get new insights into the composition and function of the pellicle polysaccharide, the polE gene of the R strain was additionally deleted, as it was reported to be involved in pellicle formation in other acetic acid bacteria.
28797225	10	2	theme	16680	1809:1813	arg1	strains					1815:1821	the other NBRC 16680 strains	1794:1821	the other NBRC 16680 strains (S, R:ΔpolE)	1794:1834	The R strain had a markedly increased tolerance towards acetic acid and ethanol compared to the other NBRC 16680 strains (S, R:ΔpolE).
28797225	10	2	theme	16680	1809:1813	arg1	S					1824:1824	S	1824:1824	S	1824:1824	The R strain had a markedly increased tolerance towards acetic acid and ethanol compared to the other NBRC 16680 strains (S, R:ΔpolE).
28797225	3	3	theme	transposon	510:519	arg1	insertion					521:529	a spontaneous transposon insertion	496:529	a spontaneous transposon insertion into the gumD gene of the gum-cluster	496:567	This switch is caused by a spontaneous transposon insertion into the gumD gene of the gum-cluster, resulting in a heteropolysaccharide secretion deficient, rough phenotype.
28797225	8	4	from	NBRC	1375:1378	arg1	change					1350:1355	The morphological change	1332:1355	The morphological change from K. baliensis NBRC 16680 S to R strain	1332:1398	The morphological change from K. baliensis NBRC 16680 S to R strain was accompanied by changes in the sugar contents of the produced pellicle EPS.
28797225	3	5	theme	gumD	540:543	arg1	gene					545:548	the gumD gene	536:548	the gumD gene of the gum-cluster	536:567	This switch is caused by a spontaneous transposon insertion into the gumD gene of the gum-cluster, resulting in a heteropolysaccharide secretion deficient, rough phenotype.
28797225	1	6	theme	baliensis	195:203	arg1	16680					210:214	BACKGROUND Kozakia baliensis NBRC 16680	176:214	BACKGROUND Kozakia baliensis NBRC 16680	176:214	BACKGROUND Kozakia baliensis NBRC 16680 secretes a gum-cluster derived heteropolysaccharide and forms a surface pellicle composed of polysaccharides during static cultivation.
28797225	11	7	theme	baliensis	2003:2011	arg1	strains					2013:2019	different K. baliensis strains	1990:2019	different K. baliensis strains	1990:2019	A relatively high intrinsic acetic acid tolerance was also observable for K. baliensis DSM 14400T, which might indicate diverse adaptation mechanisms of different K. baliensis strains in altering natural habitats.
28797225	2	8	from	types	397:401	arg1	plates					411:416	agar plates	406:416	agar plates	406:416	Furthermore, this strain exhibits two colony types on agar plates; smooth wild-type (S) and rough mutant colonies (R).
28797225	12	9	theme	phenotype	2115:2123	arg1	formation					2125:2133	the genetically triggered R phenotype formation	2087:2133	the genetically triggered R phenotype formation	2087:2133	CONCLUSION The results suggest that the genetically triggered R phenotype formation is directly related to increased acetic acid and ethanol tolerance.
28797225	12	9	theme	phenotype	2115:2123	arg1	related					2147:2153	related	2147:2153	related	2147:2153	CONCLUSION The results suggest that the genetically triggered R phenotype formation is directly related to increased acetic acid and ethanol tolerance.
28797225	6	10	theme	acid	1205:1208	arg1	bacteria					1210:1217	other acetic acid bacteria	1192:1217	other acetic acid bacteria	1192:1217	To get new insights into the composition and function of the pellicle polysaccharide, the polE gene of the R strain was additionally deleted, as it was reported to be involved in pellicle formation in other acetic acid bacteria.
28797225	11	11	theme	baliensis	1914:1922	arg1	14400T					1928:1933	K. baliensis DSM 14400T	1911:1933	K. baliensis DSM 14400T	1911:1933	A relatively high intrinsic acetic acid tolerance was also observable for K. baliensis DSM 14400T, which might indicate diverse adaptation mechanisms of different K. baliensis strains in altering natural habitats.
28797225	14	12	theme	NBRC	2566:2569	arg1	development					2538:2548	the development	2534:2548	the development of K. baliensis NBRC 16680	2534:2575	The genetic and morphological switch could represent an adaptive evolutionary step during the development of K. baliensis NBRC 16680 in course of changing environmental conditions.
28797225	13	13	theme	polysaccharide	2291:2304	arg1	formation					2250:2258	the formation	2246:2258	the formation of a cell-associated, capsular polysaccharide, which seems to be essential for increased ethanol/acetic tolerance in contrast to the secreted gum-cluster derived heteropolysaccharide	2246:2441	The polE gene turned out to be involved in the formation of a cell-associated, capsular polysaccharide, which seems to be essential for increased ethanol/acetic tolerance in contrast to the secreted gum-cluster derived heteropolysaccharide.
28797225	3	14	theme	gum-cluster	557:567	arg1	gene					545:548	the gumD gene	536:548	the gumD gene of the gum-cluster	536:567	This switch is caused by a spontaneous transposon insertion into the gumD gene of the gum-cluster, resulting in a heteropolysaccharide secretion deficient, rough phenotype.
28797225	14	15	theme	K.	2553:2554	arg1	NBRC					2566:2569	K. baliensis NBRC 16680	2553:2575	K. baliensis NBRC 16680	2553:2575	The genetic and morphological switch could represent an adaptive evolutionary step during the development of K. baliensis NBRC 16680 in course of changing environmental conditions.
28797225	9	16	theme	sugar	1617:1621	arg1	polysaccharide					1659:1672	the pellicle polysaccharide	1646:1672	the pellicle polysaccharide isolated from the R strain	1646:1699	The R:ΔpolE mutant strain was not able to form a regular pellicle anymore, but secreted an EPS into the medium, which exhibited a similar sugar monomer composition as the pellicle polysaccharide isolated from the R strain.
28797225	9	16	theme	sugar	1617:1621	arg1	composition					1631:1641	a similar sugar monomer composition	1607:1641	a similar sugar monomer composition	1607:1641	The R:ΔpolE mutant strain was not able to form a regular pellicle anymore, but secreted an EPS into the medium, which exhibited a similar sugar monomer composition as the pellicle polysaccharide isolated from the R strain.
28797225	6	17	theme	acetic	1198:1203	arg1	bacteria					1210:1217	other acetic acid bacteria	1192:1217	other acetic acid bacteria	1192:1217	To get new insights into the composition and function of the pellicle polysaccharide, the polE gene of the R strain was additionally deleted, as it was reported to be involved in pellicle formation in other acetic acid bacteria.
28797225	0	18	theme	Kozakia	146:152	arg1	NBRC					164:167	Kozakia baliensis NBRC 16680	146:173	Kozakia baliensis NBRC 16680	146:173	Environmentally triggered genomic plasticity and capsular polysaccharide formation are involved in increased ethanol and acetic acid tolerance in Kozakia baliensis NBRC 16680.
28797225	11	19	theme	natural	2033:2039	arg1	habitats					2041:2048	natural habitats	2033:2048	natural habitats	2033:2048	A relatively high intrinsic acetic acid tolerance was also observable for K. baliensis DSM 14400T, which might indicate diverse adaptation mechanisms of different K. baliensis strains in altering natural habitats.
28797225	1	20	theme	derived	239:245	arg1	heteropolysaccharide					247:266	a gum-cluster derived heteropolysaccharide	225:266	a gum-cluster derived heteropolysaccharide	225:266	BACKGROUND Kozakia baliensis NBRC 16680 secretes a gum-cluster derived heteropolysaccharide and forms a surface pellicle composed of polysaccharides during static cultivation.
28797225	11	21	theme	diverse	1957:1963	arg1	mechanisms					1976:1985	diverse adaptation mechanisms	1957:1985	diverse adaptation mechanisms of different K. baliensis strains	1957:2019	A relatively high intrinsic acetic acid tolerance was also observable for K. baliensis DSM 14400T, which might indicate diverse adaptation mechanisms of different K. baliensis strains in altering natural habitats.
28797225	10	22	dep	S	1824:1824	arg1	ΔpolE					1829:1833	ΔpolE	1829:1833	ΔpolE	1829:1833	The R strain had a markedly increased tolerance towards acetic acid and ethanol compared to the other NBRC 16680 strains (S, R:ΔpolE).
28797225	10	22	dep	S	1824:1824	arg1	R					1827:1827	R	1827:1827	R	1827:1827	The R strain had a markedly increased tolerance towards acetic acid and ethanol compared to the other NBRC 16680 strains (S, R:ΔpolE).
28797225	13	23	theme	increased	2339:2347	arg1	tolerance					2364:2372	increased ethanol/acetic tolerance	2339:2372	increased ethanol/acetic tolerance in contrast to the secreted gum-cluster derived heteropolysaccharide	2339:2441	The polE gene turned out to be involved in the formation of a cell-associated, capsular polysaccharide, which seems to be essential for increased ethanol/acetic tolerance in contrast to the secreted gum-cluster derived heteropolysaccharide.
28797225	10	24	theme	R	1706:1706	arg1	strain					1708:1713	The R strain	1702:1713	The R strain	1702:1713	The R strain had a markedly increased tolerance towards acetic acid and ethanol compared to the other NBRC 16680 strains (S, R:ΔpolE).
28797225	5	25	theme	strains	909:915	arg1	tolerance					888:896	the tolerance	884:896	the tolerance of R and S strains against ethanol and acetic acid in shaking and static growth experiments	884:988	Furthermore, we investigated the tolerance of R and S strains against ethanol and acetic acid in shaking and static growth experiments.
28797225	6	26	theme	pellicle	1170:1177	arg1	formation					1179:1187	pellicle formation	1170:1187	pellicle formation in other acetic acid bacteria	1170:1217	To get new insights into the composition and function of the pellicle polysaccharide, the polE gene of the R strain was additionally deleted, as it was reported to be involved in pellicle formation in other acetic acid bacteria.
28797225	6	27	from	formation	1179:1187	arg1	bacteria					1210:1217	other acetic acid bacteria	1192:1217	other acetic acid bacteria	1192:1217	To get new insights into the composition and function of the pellicle polysaccharide, the polE gene of the R strain was additionally deleted, as it was reported to be involved in pellicle formation in other acetic acid bacteria.
28797225	11	28	theme	acetic	1865:1870	arg1	tolerance					1877:1885	A relatively high intrinsic acetic acid tolerance	1837:1885	A relatively high intrinsic acetic acid tolerance	1837:1885	A relatively high intrinsic acetic acid tolerance was also observable for K. baliensis DSM 14400T, which might indicate diverse adaptation mechanisms of different K. baliensis strains in altering natural habitats.
28797225	11	28	theme	acetic	1865:1870	arg1	observable					1896:1905	observable	1896:1905	observable	1896:1905	A relatively high intrinsic acetic acid tolerance was also observable for K. baliensis DSM 14400T, which might indicate diverse adaptation mechanisms of different K. baliensis strains in altering natural habitats.
28797225	13	29	from	tolerance	2364:2372	arg1	contrast					2377:2384	contrast	2377:2384	contrast	2377:2384	The polE gene turned out to be involved in the formation of a cell-associated, capsular polysaccharide, which seems to be essential for increased ethanol/acetic tolerance in contrast to the secreted gum-cluster derived heteropolysaccharide.
28797225	8	30	theme	sugar	1434:1438	arg1	contents					1440:1447	the sugar contents	1430:1447	the sugar contents of the produced pellicle EPS	1430:1476	The morphological change from K. baliensis NBRC 16680 S to R strain was accompanied by changes in the sugar contents of the produced pellicle EPS.
28797225	11	31	theme	high	1850:1853	arg1	tolerance					1877:1885	A relatively high intrinsic acetic acid tolerance	1837:1885	A relatively high intrinsic acetic acid tolerance	1837:1885	A relatively high intrinsic acetic acid tolerance was also observable for K. baliensis DSM 14400T, which might indicate diverse adaptation mechanisms of different K. baliensis strains in altering natural habitats.
28797225	11	31	theme	high	1850:1853	arg1	observable					1896:1905	observable	1896:1905	observable	1896:1905	A relatively high intrinsic acetic acid tolerance was also observable for K. baliensis DSM 14400T, which might indicate diverse adaptation mechanisms of different K. baliensis strains in altering natural habitats.
28797225	6	32	theme	new	998:1000	arg1	insights					1002:1009	new insights	998:1009	new insights into the composition and function of the pellicle polysaccharide	998:1074	To get new insights into the composition and function of the pellicle polysaccharide, the polE gene of the R strain was additionally deleted, as it was reported to be involved in pellicle formation in other acetic acid bacteria.
28797225	1	33	theme	BACKGROUND	176:185	arg1	16680					210:214	BACKGROUND Kozakia baliensis NBRC 16680	176:214	BACKGROUND Kozakia baliensis NBRC 16680	176:214	BACKGROUND Kozakia baliensis NBRC 16680 secretes a gum-cluster derived heteropolysaccharide and forms a surface pellicle composed of polysaccharides during static cultivation.
28797225	9	34	theme	R	1692:1692	arg1	strain					1694:1699	the R strain	1688:1699	the R strain	1688:1699	The R:ΔpolE mutant strain was not able to form a regular pellicle anymore, but secreted an EPS into the medium, which exhibited a similar sugar monomer composition as the pellicle polysaccharide isolated from the R strain.
28797225	8	35	theme	produced	1456:1463	arg1	EPS					1474:1476	the produced pellicle EPS	1452:1476	the produced pellicle EPS	1452:1476	The morphological change from K. baliensis NBRC 16680 S to R strain was accompanied by changes in the sugar contents of the produced pellicle EPS.
28797225	12	36	theme	triggered	2103:2111	arg1	formation					2125:2133	the genetically triggered R phenotype formation	2087:2133	the genetically triggered R phenotype formation	2087:2133	CONCLUSION The results suggest that the genetically triggered R phenotype formation is directly related to increased acetic acid and ethanol tolerance.
28797225	12	36	theme	triggered	2103:2111	arg1	related					2147:2153	related	2147:2153	related	2147:2153	CONCLUSION The results suggest that the genetically triggered R phenotype formation is directly related to increased acetic acid and ethanol tolerance.
28797225	0	37	theme	ethanol	109:115	arg1	tolerance					133:141	increased ethanol and acetic acid tolerance	99:141	increased ethanol and acetic acid tolerance in Kozakia baliensis NBRC 16680	99:173	Environmentally triggered genomic plasticity and capsular polysaccharide formation are involved in increased ethanol and acetic acid tolerance in Kozakia baliensis NBRC 16680.
28797225	9	38	theme	ΔpolE	1485:1489	arg1	strain					1498:1503	The R:ΔpolE mutant strain	1479:1503	The R:ΔpolE mutant strain	1479:1503	The R:ΔpolE mutant strain was not able to form a regular pellicle anymore, but secreted an EPS into the medium, which exhibited a similar sugar monomer composition as the pellicle polysaccharide isolated from the R strain.
28797225	9	38	theme	ΔpolE	1485:1489	arg1	able					1513:1516	able	1513:1516	able	1513:1516	The R:ΔpolE mutant strain was not able to form a regular pellicle anymore, but secreted an EPS into the medium, which exhibited a similar sugar monomer composition as the pellicle polysaccharide isolated from the R strain.
28797225	14	39	theme	morphological	2460:2472	arg1	switch					2474:2479	The genetic and morphological switch	2444:2479	The genetic and morphological switch	2444:2479	The genetic and morphological switch could represent an adaptive evolutionary step during the development of K. baliensis NBRC 16680 in course of changing environmental conditions.
28797225	8	40	theme	EPS	1474:1476	arg1	contents					1440:1447	the sugar contents	1430:1447	the sugar contents of the produced pellicle EPS	1430:1476	The morphological change from K. baliensis NBRC 16680 S to R strain was accompanied by changes in the sugar contents of the produced pellicle EPS.
28797225	4	41	theme	colonies	761:768	arg1	number					743:748	the number	739:748	the number of R and S colonies	739:768	To elucidate, whether this is a directed switch triggered by environmental factors, we checked the number of R and S colonies under different growth conditions including ethanol and acetic acid supplementation.
28797225	0	42	theme	acetic	121:126	arg1	acid					128:131	acetic acid	121:131	acetic acid	121:131	Environmentally triggered genomic plasticity and capsular polysaccharide formation are involved in increased ethanol and acetic acid tolerance in Kozakia baliensis NBRC 16680.
28797225	5	43	theme	growth	971:976	arg1	experiments					978:988	static growth experiments	964:988	static growth experiments	964:988	Furthermore, we investigated the tolerance of R and S strains against ethanol and acetic acid in shaking and static growth experiments.
28797225	6	44	theme	polysaccharide	1061:1074	arg1	function					1036:1043	function	1036:1043	function	1036:1043	To get new insights into the composition and function of the pellicle polysaccharide, the polE gene of the R strain was additionally deleted, as it was reported to be involved in pellicle formation in other acetic acid bacteria.
28797225	6	44	theme	polysaccharide	1061:1074	arg1	composition					1020:1030	composition	1020:1030	composition	1020:1030	To get new insights into the composition and function of the pellicle polysaccharide, the polE gene of the R strain was additionally deleted, as it was reported to be involved in pellicle formation in other acetic acid bacteria.
28797225	12	45	theme	increased	2158:2166	arg1	acid					2175:2178	increased acetic acid	2158:2178	increased acetic acid	2158:2178	CONCLUSION The results suggest that the genetically triggered R phenotype formation is directly related to increased acetic acid and ethanol tolerance.
28797225	2	46	theme	smooth	419:424	arg1	R					467:467	R	467:467	R	467:467	Furthermore, this strain exhibits two colony types on agar plates; smooth wild-type (S) and rough mutant colonies (R).
28797225	2	46	theme	smooth	419:424	arg1	colonies					457:464	smooth wild-type (S) and rough mutant colonies	419:464	two colony types on agar plates; smooth wild-type (S) and rough mutant colonies (R)	386:468	Furthermore, this strain exhibits two colony types on agar plates; smooth wild-type (S) and rough mutant colonies (R).
28797225	5	47	theme	acetic	937:942	arg1	acid					944:947	acetic acid	937:947	acetic acid	937:947	Furthermore, we investigated the tolerance of R and S strains against ethanol and acetic acid in shaking and static growth experiments.
28797225	14	48	theme	genetic	2448:2454	arg1	switch					2474:2479	The genetic and morphological switch	2444:2479	The genetic and morphological switch	2444:2479	The genetic and morphological switch could represent an adaptive evolutionary step during the development of K. baliensis NBRC 16680 in course of changing environmental conditions.
28797225	10	49	contain	had	1715:1717	arg2	tolerance					1740:1748	a markedly increased tolerance	1719:1748	a markedly increased tolerance towards acetic acid and ethanol compared to the other NBRC 16680 strains (S, R:ΔpolE)	1719:1834	The R strain had a markedly increased tolerance towards acetic acid and ethanol compared to the other NBRC 16680 strains (S, R:ΔpolE).
28797225	10	49	contain	had	1715:1717	arg1	strain					1708:1713	The R strain	1702:1713	The R strain	1702:1713	The R strain had a markedly increased tolerance towards acetic acid and ethanol compared to the other NBRC 16680 strains (S, R:ΔpolE).
28797225	10	50	theme	increased	1730:1738	arg1	tolerance					1740:1748	a markedly increased tolerance	1719:1748	a markedly increased tolerance towards acetic acid and ethanol compared to the other NBRC 16680 strains (S, R:ΔpolE)	1719:1834	The R strain had a markedly increased tolerance towards acetic acid and ethanol compared to the other NBRC 16680 strains (S, R:ΔpolE).
28797225	12	51	theme	R	2113:2113	arg1	formation					2125:2133	the genetically triggered R phenotype formation	2087:2133	the genetically triggered R phenotype formation	2087:2133	CONCLUSION The results suggest that the genetically triggered R phenotype formation is directly related to increased acetic acid and ethanol tolerance.
28797225	12	51	theme	R	2113:2113	arg1	related					2147:2153	related	2147:2153	related	2147:2153	CONCLUSION The results suggest that the genetically triggered R phenotype formation is directly related to increased acetic acid and ethanol tolerance.
28797225	0	52	theme	genomic	26:32	arg1	plasticity					34:43	Environmentally triggered genomic plasticity	0:43	Environmentally triggered genomic plasticity	0:43	Environmentally triggered genomic plasticity and capsular polysaccharide formation are involved in increased ethanol and acetic acid tolerance in Kozakia baliensis NBRC 16680.
28797225	1	53	theme	static	332:337	arg1	cultivation					339:349	static cultivation	332:349	static cultivation	332:349	BACKGROUND Kozakia baliensis NBRC 16680 secretes a gum-cluster derived heteropolysaccharide and forms a surface pellicle composed of polysaccharides during static cultivation.
28797225	7	54	dep	RESULTS	1220:1226	arg1	increased					1271:1279	increased	1271:1279	was significantly increased upon growth on acetic acid and especially ethanol	1253:1329	RESULTS The number of R colonies was significantly increased upon growth on acetic acid and especially ethanol.
28797225	12	55	dep	CONCLUSION	2051:2060	arg1	suggest					2074:2080	suggest	2074:2080	suggest that the genetically triggered R phenotype formation is directly related to increased acetic acid and ethanol tolerance	2074:2200	CONCLUSION The results suggest that the genetically triggered R phenotype formation is directly related to increased acetic acid and ethanol tolerance.
28797225	2	56	dep	wild-type	426:434	arg1	S					437:437	S	437:437	S	437:437	Furthermore, this strain exhibits two colony types on agar plates; smooth wild-type (S) and rough mutant colonies (R).
28797225	10	57	theme	acetic	1758:1763	arg1	acid					1765:1768	acetic acid	1758:1768	acetic acid	1758:1768	The R strain had a markedly increased tolerance towards acetic acid and ethanol compared to the other NBRC 16680 strains (S, R:ΔpolE).
28797225	4	58	theme	acetic	826:831	arg1	supplementation					838:852	acetic acid supplementation	826:852	acetic acid supplementation	826:852	To elucidate, whether this is a directed switch triggered by environmental factors, we checked the number of R and S colonies under different growth conditions including ethanol and acetic acid supplementation.
28797225	2	59	theme	colony	390:395	arg1	types					397:401	two colony types	386:401	two colony types on agar plates; smooth wild-type (S) and rough mutant colonies (R)	386:468	Furthermore, this strain exhibits two colony types on agar plates; smooth wild-type (S) and rough mutant colonies (R).
28797225	0	60	theme	polysaccharide	58:71	arg1	formation					73:81	capsular polysaccharide formation	49:81	capsular polysaccharide formation	49:81	Environmentally triggered genomic plasticity and capsular polysaccharide formation are involved in increased ethanol and acetic acid tolerance in Kozakia baliensis NBRC 16680.
28797225	9	61	theme	pellicle	1536:1543	arg1	anymore					1545:1551	a regular pellicle anymore	1526:1551	a regular pellicle anymore	1526:1551	The R:ΔpolE mutant strain was not able to form a regular pellicle anymore, but secreted an EPS into the medium, which exhibited a similar sugar monomer composition as the pellicle polysaccharide isolated from the R strain.
28797225	6	62	theme	strain	1100:1105	arg1	gene					1086:1089	the polE gene	1077:1089	the polE gene of the R strain	1077:1105	To get new insights into the composition and function of the pellicle polysaccharide, the polE gene of the R strain was additionally deleted, as it was reported to be involved in pellicle formation in other acetic acid bacteria.
28797225	4	63	theme	different	776:784	arg1	ethanol					814:820	ethanol	814:820	ethanol	814:820	To elucidate, whether this is a directed switch triggered by environmental factors, we checked the number of R and S colonies under different growth conditions including ethanol and acetic acid supplementation.
28797225	4	63	theme	different	776:784	arg1	conditions					793:802	different growth conditions	776:802	different growth conditions including ethanol and acetic acid supplementation	776:852	To elucidate, whether this is a directed switch triggered by environmental factors, we checked the number of R and S colonies under different growth conditions including ethanol and acetic acid supplementation.
28797225	4	63	theme	different	776:784	arg1	supplementation					838:852	acetic acid supplementation	826:852	acetic acid supplementation	826:852	To elucidate, whether this is a directed switch triggered by environmental factors, we checked the number of R and S colonies under different growth conditions including ethanol and acetic acid supplementation.
28797225	14	64	theme	environmental	2599:2611	arg1	conditions					2613:2622	environmental conditions	2599:2622	environmental conditions	2599:2622	The genetic and morphological switch could represent an adaptive evolutionary step during the development of K. baliensis NBRC 16680 in course of changing environmental conditions.
28797225	0	65	from	tolerance	133:141	arg1	NBRC					164:167	Kozakia baliensis NBRC 16680	146:173	Kozakia baliensis NBRC 16680	146:173	Environmentally triggered genomic plasticity and capsular polysaccharide formation are involved in increased ethanol and acetic acid tolerance in Kozakia baliensis NBRC 16680.
28797225	14	66	from	step	2522:2525	arg1	course					2580:2585	course	2580:2585	course of changing environmental conditions	2580:2622	The genetic and morphological switch could represent an adaptive evolutionary step during the development of K. baliensis NBRC 16680 in course of changing environmental conditions.
28797225	3	67	theme	spontaneous	498:508	arg1	insertion					521:529	a spontaneous transposon insertion	496:529	a spontaneous transposon insertion into the gumD gene of the gum-cluster	496:567	This switch is caused by a spontaneous transposon insertion into the gumD gene of the gum-cluster, resulting in a heteropolysaccharide secretion deficient, rough phenotype.
28797225	1	68	theme	surface	280:286	arg1	pellicle					288:295	a surface pellicle	278:295	a surface pellicle composed of polysaccharides during static cultivation	278:349	BACKGROUND Kozakia baliensis NBRC 16680 secretes a gum-cluster derived heteropolysaccharide and forms a surface pellicle composed of polysaccharides during static cultivation.
28797225	11	69	theme	K.	2000:2001	arg1	strains					2013:2019	different K. baliensis strains	1990:2019	different K. baliensis strains	1990:2019	A relatively high intrinsic acetic acid tolerance was also observable for K. baliensis DSM 14400T, which might indicate diverse adaptation mechanisms of different K. baliensis strains in altering natural habitats.
28797225	10	70	theme	NBRC	1804:1807	arg1	strains					1815:1821	the other NBRC 16680 strains	1794:1821	the other NBRC 16680 strains (S, R:ΔpolE)	1794:1834	The R strain had a markedly increased tolerance towards acetic acid and ethanol compared to the other NBRC 16680 strains (S, R:ΔpolE).
28797225	10	70	theme	NBRC	1804:1807	arg1	S					1824:1824	S	1824:1824	S	1824:1824	The R strain had a markedly increased tolerance towards acetic acid and ethanol compared to the other NBRC 16680 strains (S, R:ΔpolE).
28797225	13	71	theme	cell-associated	2265:2279	arg1	polysaccharide					2291:2304	a cell-associated, capsular polysaccharide	2263:2304	polysaccharide	2291:2304	The polE gene turned out to be involved in the formation of a cell-associated, capsular polysaccharide, which seems to be essential for increased ethanol/acetic tolerance in contrast to the secreted gum-cluster derived heteropolysaccharide.
28797225	13	71	theme	cell-associated	2265:2279	arg1	essential					2325:2333	essential	2325:2333	essential	2325:2333	The polE gene turned out to be involved in the formation of a cell-associated, capsular polysaccharide, which seems to be essential for increased ethanol/acetic tolerance in contrast to the secreted gum-cluster derived heteropolysaccharide.
28797225	9	72	attach	isolated	1674:1681	arg2	polysaccharide					1659:1672	the pellicle polysaccharide	1646:1672	the pellicle polysaccharide isolated from the R strain	1646:1699	The R:ΔpolE mutant strain was not able to form a regular pellicle anymore, but secreted an EPS into the medium, which exhibited a similar sugar monomer composition as the pellicle polysaccharide isolated from the R strain.
28797225	9	72	attach	isolated	1674:1681	arg2	composition					1631:1641	a similar sugar monomer composition	1607:1641	a similar sugar monomer composition	1607:1641	The R:ΔpolE mutant strain was not able to form a regular pellicle anymore, but secreted an EPS into the medium, which exhibited a similar sugar monomer composition as the pellicle polysaccharide isolated from the R strain.
28797225	9	72	attach	isolated	1674:1681	arg1	strain					1694:1699	the R strain	1688:1699	the R strain	1688:1699	The R:ΔpolE mutant strain was not able to form a regular pellicle anymore, but secreted an EPS into the medium, which exhibited a similar sugar monomer composition as the pellicle polysaccharide isolated from the R strain.
28797225	14	73	theme	evolutionary	2509:2520	arg1	step					2522:2525	an adaptive evolutionary step	2497:2525	an adaptive evolutionary step during the development of K. baliensis NBRC 16680 in course of changing environmental conditions	2497:2622	The genetic and morphological switch could represent an adaptive evolutionary step during the development of K. baliensis NBRC 16680 in course of changing environmental conditions.
28797225	13	74	theme	secreted	2393:2400	arg1	gum-cluster					2402:2412	the secreted gum-cluster	2389:2412	the secreted gum-cluster derived heteropolysaccharide	2389:2441	The polE gene turned out to be involved in the formation of a cell-associated, capsular polysaccharide, which seems to be essential for increased ethanol/acetic tolerance in contrast to the secreted gum-cluster derived heteropolysaccharide.
28797225	11	75	theme	acid	1872:1875	arg1	tolerance					1877:1885	A relatively high intrinsic acetic acid tolerance	1837:1885	A relatively high intrinsic acetic acid tolerance	1837:1885	A relatively high intrinsic acetic acid tolerance was also observable for K. baliensis DSM 14400T, which might indicate diverse adaptation mechanisms of different K. baliensis strains in altering natural habitats.
28797225	11	75	theme	acid	1872:1875	arg1	observable					1896:1905	observable	1896:1905	observable	1896:1905	A relatively high intrinsic acetic acid tolerance was also observable for K. baliensis DSM 14400T, which might indicate diverse adaptation mechanisms of different K. baliensis strains in altering natural habitats.
28797225	1	76	theme	Kozakia	187:193	arg1	16680					210:214	BACKGROUND Kozakia baliensis NBRC 16680	176:214	BACKGROUND Kozakia baliensis NBRC 16680	176:214	BACKGROUND Kozakia baliensis NBRC 16680 secretes a gum-cluster derived heteropolysaccharide and forms a surface pellicle composed of polysaccharides during static cultivation.
28797225	4	77	theme	acid	833:836	arg1	supplementation					838:852	acetic acid supplementation	826:852	acetic acid supplementation	826:852	To elucidate, whether this is a directed switch triggered by environmental factors, we checked the number of R and S colonies under different growth conditions including ethanol and acetic acid supplementation.
28797225	11	78	theme	K.	1911:1912	arg1	14400T					1928:1933	K. baliensis DSM 14400T	1911:1933	K. baliensis DSM 14400T	1911:1933	A relatively high intrinsic acetic acid tolerance was also observable for K. baliensis DSM 14400T, which might indicate diverse adaptation mechanisms of different K. baliensis strains in altering natural habitats.
28797225	1	79	theme	NBRC	205:208	arg1	16680					210:214	BACKGROUND Kozakia baliensis NBRC 16680	176:214	BACKGROUND Kozakia baliensis NBRC 16680	176:214	BACKGROUND Kozakia baliensis NBRC 16680 secretes a gum-cluster derived heteropolysaccharide and forms a surface pellicle composed of polysaccharides during static cultivation.
28797225	11	80	theme	strains	2013:2019	arg1	mechanisms					1976:1985	diverse adaptation mechanisms	1957:1985	diverse adaptation mechanisms of different K. baliensis strains	1957:2019	A relatively high intrinsic acetic acid tolerance was also observable for K. baliensis DSM 14400T, which might indicate diverse adaptation mechanisms of different K. baliensis strains in altering natural habitats.
28797225	3	81	dep	deficient	616:624	arg1	rough					627:631	rough	627:631	rough	627:631	This switch is caused by a spontaneous transposon insertion into the gumD gene of the gum-cluster, resulting in a heteropolysaccharide secretion deficient, rough phenotype.
28797225	14	82	theme	baliensis	2556:2564	arg1	NBRC					2566:2569	K. baliensis NBRC 16680	2553:2575	K. baliensis NBRC 16680	2553:2575	The genetic and morphological switch could represent an adaptive evolutionary step during the development of K. baliensis NBRC 16680 in course of changing environmental conditions.
28797225	9	83	theme	similar	1609:1615	arg1	polysaccharide					1659:1672	the pellicle polysaccharide	1646:1672	the pellicle polysaccharide isolated from the R strain	1646:1699	The R:ΔpolE mutant strain was not able to form a regular pellicle anymore, but secreted an EPS into the medium, which exhibited a similar sugar monomer composition as the pellicle polysaccharide isolated from the R strain.
28797225	9	83	theme	similar	1609:1615	arg1	composition					1631:1641	a similar sugar monomer composition	1607:1641	a similar sugar monomer composition	1607:1641	The R:ΔpolE mutant strain was not able to form a regular pellicle anymore, but secreted an EPS into the medium, which exhibited a similar sugar monomer composition as the pellicle polysaccharide isolated from the R strain.
28797225	9	84	theme	R	1483:1483	arg1	strain					1498:1503	The R:ΔpolE mutant strain	1479:1503	The R:ΔpolE mutant strain	1479:1503	The R:ΔpolE mutant strain was not able to form a regular pellicle anymore, but secreted an EPS into the medium, which exhibited a similar sugar monomer composition as the pellicle polysaccharide isolated from the R strain.
28797225	9	84	theme	R	1483:1483	arg1	able					1513:1516	able	1513:1516	able	1513:1516	The R:ΔpolE mutant strain was not able to form a regular pellicle anymore, but secreted an EPS into the medium, which exhibited a similar sugar monomer composition as the pellicle polysaccharide isolated from the R strain.
28797225	7	85	theme	R	1242:1242	arg1	colonies					1244:1251	R colonies	1242:1251	R colonies	1242:1251	RESULTS The number of R colonies was significantly increased upon growth on acetic acid and especially ethanol.
28797225	0	86	theme	baliensis	154:162	arg1	NBRC					164:167	Kozakia baliensis NBRC 16680	146:173	Kozakia baliensis NBRC 16680	146:173	Environmentally triggered genomic plasticity and capsular polysaccharide formation are involved in increased ethanol and acetic acid tolerance in Kozakia baliensis NBRC 16680.
28797225	1	87	theme	gum-cluster	227:237	arg1	heteropolysaccharide					247:266	a gum-cluster derived heteropolysaccharide	225:266	a gum-cluster derived heteropolysaccharide	225:266	BACKGROUND Kozakia baliensis NBRC 16680 secretes a gum-cluster derived heteropolysaccharide and forms a surface pellicle composed of polysaccharides during static cultivation.
28797225	9	88	theme	monomer	1623:1629	arg1	polysaccharide					1659:1672	the pellicle polysaccharide	1646:1672	the pellicle polysaccharide isolated from the R strain	1646:1699	The R:ΔpolE mutant strain was not able to form a regular pellicle anymore, but secreted an EPS into the medium, which exhibited a similar sugar monomer composition as the pellicle polysaccharide isolated from the R strain.
28797225	9	88	theme	monomer	1623:1629	arg1	composition					1631:1641	a similar sugar monomer composition	1607:1641	a similar sugar monomer composition	1607:1641	The R:ΔpolE mutant strain was not able to form a regular pellicle anymore, but secreted an EPS into the medium, which exhibited a similar sugar monomer composition as the pellicle polysaccharide isolated from the R strain.
28797225	6	89	theme	other	1192:1196	arg1	bacteria					1210:1217	other acetic acid bacteria	1192:1217	other acetic acid bacteria	1192:1217	To get new insights into the composition and function of the pellicle polysaccharide, the polE gene of the R strain was additionally deleted, as it was reported to be involved in pellicle formation in other acetic acid bacteria.
28797225	5	90	theme	S	907:907	arg1	strains					909:915	R and S strains	901:915	R and S strains	901:915	Furthermore, we investigated the tolerance of R and S strains against ethanol and acetic acid in shaking and static growth experiments.
28797225	12	91	theme	ethanol	2184:2190	arg1	tolerance					2192:2200	ethanol tolerance	2184:2200	ethanol tolerance	2184:2200	CONCLUSION The results suggest that the genetically triggered R phenotype formation is directly related to increased acetic acid and ethanol tolerance.
28797225	11	92	theme	adaptation	1965:1974	arg1	mechanisms					1976:1985	diverse adaptation mechanisms	1957:1985	diverse adaptation mechanisms of different K. baliensis strains	1957:2019	A relatively high intrinsic acetic acid tolerance was also observable for K. baliensis DSM 14400T, which might indicate diverse adaptation mechanisms of different K. baliensis strains in altering natural habitats.
28797225	4	93	theme	environmental	705:717	arg1	factors					719:725	environmental factors	705:725	environmental factors	705:725	To elucidate, whether this is a directed switch triggered by environmental factors, we checked the number of R and S colonies under different growth conditions including ethanol and acetic acid supplementation.
28797225	13	94	theme	ethanol/acetic	2349:2362	arg1	tolerance					2364:2372	increased ethanol/acetic tolerance	2339:2372	increased ethanol/acetic tolerance in contrast to the secreted gum-cluster derived heteropolysaccharide	2339:2441	The polE gene turned out to be involved in the formation of a cell-associated, capsular polysaccharide, which seems to be essential for increased ethanol/acetic tolerance in contrast to the secreted gum-cluster derived heteropolysaccharide.
28797225	9	95	theme	pellicle	1650:1657	arg1	polysaccharide					1659:1672	the pellicle polysaccharide	1646:1672	the pellicle polysaccharide isolated from the R strain	1646:1699	The R:ΔpolE mutant strain was not able to form a regular pellicle anymore, but secreted an EPS into the medium, which exhibited a similar sugar monomer composition as the pellicle polysaccharide isolated from the R strain.
28797225	9	95	theme	pellicle	1650:1657	arg1	composition					1631:1641	a similar sugar monomer composition	1607:1641	a similar sugar monomer composition	1607:1641	The R:ΔpolE mutant strain was not able to form a regular pellicle anymore, but secreted an EPS into the medium, which exhibited a similar sugar monomer composition as the pellicle polysaccharide isolated from the R strain.
28797225	8	96	from	changes	1419:1425	arg1	contents					1440:1447	the sugar contents	1430:1447	the sugar contents of the produced pellicle EPS	1430:1476	The morphological change from K. baliensis NBRC 16680 S to R strain was accompanied by changes in the sugar contents of the produced pellicle EPS.
28797225	3	97	theme	deficient	616:624	arg1	phenotype					633:641	a heteropolysaccharide secretion deficient, rough phenotype	583:641	a heteropolysaccharide secretion deficient, rough phenotype	583:641	This switch is caused by a spontaneous transposon insertion into the gumD gene of the gum-cluster, resulting in a heteropolysaccharide secretion deficient, rough phenotype.
28797225	14	98	theme	adaptive	2500:2507	arg1	step					2522:2525	an adaptive evolutionary step	2497:2525	an adaptive evolutionary step during the development of K. baliensis NBRC 16680 in course of changing environmental conditions	2497:2622	The genetic and morphological switch could represent an adaptive evolutionary step during the development of K. baliensis NBRC 16680 in course of changing environmental conditions.
28797225	4	99	theme	directed	676:683	arg1	this					666:669	this	666:669	this	666:669	To elucidate, whether this is a directed switch triggered by environmental factors, we checked the number of R and S colonies under different growth conditions including ethanol and acetic acid supplementation.
28797225	4	99	theme	directed	676:683	arg1	switch					685:690	a directed switch	674:690	a directed switch triggered by environmental factors	674:725	To elucidate, whether this is a directed switch triggered by environmental factors, we checked the number of R and S colonies under different growth conditions including ethanol and acetic acid supplementation.
28797225	0	100	theme	increased	99:107	arg1	tolerance					133:141	increased ethanol and acetic acid tolerance	99:141	increased ethanol and acetic acid tolerance in Kozakia baliensis NBRC 16680	99:173	Environmentally triggered genomic plasticity and capsular polysaccharide formation are involved in increased ethanol and acetic acid tolerance in Kozakia baliensis NBRC 16680.
28797225	5	101	theme	R	901:901	arg1	strains					909:915	R and S strains	901:915	R and S strains	901:915	Furthermore, we investigated the tolerance of R and S strains against ethanol and acetic acid in shaking and static growth experiments.
28797225	8	102	theme	pellicle	1465:1472	arg1	EPS					1474:1476	the produced pellicle EPS	1452:1476	the produced pellicle EPS	1452:1476	The morphological change from K. baliensis NBRC 16680 S to R strain was accompanied by changes in the sugar contents of the produced pellicle EPS.
28797225	1	103	link	derived	239:245	arg1	heteropolysaccharide					247:266	a gum-cluster derived heteropolysaccharide	225:266	a gum-cluster derived heteropolysaccharide	225:266	BACKGROUND Kozakia baliensis NBRC 16680 secretes a gum-cluster derived heteropolysaccharide and forms a surface pellicle composed of polysaccharides during static cultivation.
28797225	2	104	dep	types	397:401	arg1	R					467:467	R	467:467	R	467:467	Furthermore, this strain exhibits two colony types on agar plates; smooth wild-type (S) and rough mutant colonies (R).
28797225	2	104	dep	types	397:401	arg1	colonies					457:464	smooth wild-type (S) and rough mutant colonies	419:464	two colony types on agar plates; smooth wild-type (S) and rough mutant colonies (R)	386:468	Furthermore, this strain exhibits two colony types on agar plates; smooth wild-type (S) and rough mutant colonies (R).
28797225	4	105	theme	R	753:753	arg1	colonies					761:768	R and S colonies	753:768	R and S colonies	753:768	To elucidate, whether this is a directed switch triggered by environmental factors, we checked the number of R and S colonies under different growth conditions including ethanol and acetic acid supplementation.
28797225	0	106	theme	acid	128:131	arg1	tolerance					133:141	increased ethanol and acetic acid tolerance	99:141	increased ethanol and acetic acid tolerance in Kozakia baliensis NBRC 16680	99:173	Environmentally triggered genomic plasticity and capsular polysaccharide formation are involved in increased ethanol and acetic acid tolerance in Kozakia baliensis NBRC 16680.
28797225	5	107	theme	static	964:969	arg1	experiments					978:988	static growth experiments	964:988	static growth experiments	964:988	Furthermore, we investigated the tolerance of R and S strains against ethanol and acetic acid in shaking and static growth experiments.
28797225	12	108	theme	acetic	2168:2173	arg1	acid					2175:2178	increased acetic acid	2158:2178	increased acetic acid	2158:2178	CONCLUSION The results suggest that the genetically triggered R phenotype formation is directly related to increased acetic acid and ethanol tolerance.
28797225	4	109	theme	S	759:759	arg1	colonies					761:768	R and S colonies	753:768	R and S colonies	753:768	To elucidate, whether this is a directed switch triggered by environmental factors, we checked the number of R and S colonies under different growth conditions including ethanol and acetic acid supplementation.
28797225	2	110	theme	wild-type	426:434	arg1	R					467:467	R	467:467	R	467:467	Furthermore, this strain exhibits two colony types on agar plates; smooth wild-type (S) and rough mutant colonies (R).
28797225	2	110	theme	wild-type	426:434	arg1	colonies					457:464	smooth wild-type (S) and rough mutant colonies	419:464	two colony types on agar plates; smooth wild-type (S) and rough mutant colonies (R)	386:468	Furthermore, this strain exhibits two colony types on agar plates; smooth wild-type (S) and rough mutant colonies (R).
28797225	0	111	theme	triggered	16:24	arg1	plasticity					34:43	Environmentally triggered genomic plasticity	0:43	Environmentally triggered genomic plasticity	0:43	Environmentally triggered genomic plasticity and capsular polysaccharide formation are involved in increased ethanol and acetic acid tolerance in Kozakia baliensis NBRC 16680.
28797225	5	112	from	ethanol	925:931	arg1	experiments					978:988	static growth experiments	964:988	static growth experiments	964:988	Furthermore, we investigated the tolerance of R and S strains against ethanol and acetic acid in shaking and static growth experiments.
28797225	5	112	from	ethanol	925:931	arg1	shaking					952:958	shaking	952:958	shaking	952:958	Furthermore, we investigated the tolerance of R and S strains against ethanol and acetic acid in shaking and static growth experiments.
28797225	13	113	theme	capsular	2282:2289	arg1	polysaccharide					2291:2304	a cell-associated, capsular polysaccharide	2263:2304	polysaccharide	2291:2304	The polE gene turned out to be involved in the formation of a cell-associated, capsular polysaccharide, which seems to be essential for increased ethanol/acetic tolerance in contrast to the secreted gum-cluster derived heteropolysaccharide.
28797225	13	113	theme	capsular	2282:2289	arg1	essential					2325:2333	essential	2325:2333	essential	2325:2333	The polE gene turned out to be involved in the formation of a cell-associated, capsular polysaccharide, which seems to be essential for increased ethanol/acetic tolerance in contrast to the secreted gum-cluster derived heteropolysaccharide.
28797225	6	114	theme	pellicle	1052:1059	arg1	polysaccharide					1061:1074	the pellicle polysaccharide	1048:1074	the pellicle polysaccharide	1048:1074	To get new insights into the composition and function of the pellicle polysaccharide, the polE gene of the R strain was additionally deleted, as it was reported to be involved in pellicle formation in other acetic acid bacteria.
28797225	11	115	theme	DSM	1924:1926	arg1	14400T					1928:1933	K. baliensis DSM 14400T	1911:1933	K. baliensis DSM 14400T	1911:1933	A relatively high intrinsic acetic acid tolerance was also observable for K. baliensis DSM 14400T, which might indicate diverse adaptation mechanisms of different K. baliensis strains in altering natural habitats.
28797225	2	116	theme	mutant	450:455	arg1	R					467:467	R	467:467	R	467:467	Furthermore, this strain exhibits two colony types on agar plates; smooth wild-type (S) and rough mutant colonies (R).
28797225	2	116	theme	mutant	450:455	arg1	colonies					457:464	smooth wild-type (S) and rough mutant colonies	419:464	two colony types on agar plates; smooth wild-type (S) and rough mutant colonies (R)	386:468	Furthermore, this strain exhibits two colony types on agar plates; smooth wild-type (S) and rough mutant colonies (R).
28797225	6	117	dep	composition	1020:1030	arg1	the					1016:1018	the	1016:1018	the	1016:1018	To get new insights into the composition and function of the pellicle polysaccharide, the polE gene of the R strain was additionally deleted, as it was reported to be involved in pellicle formation in other acetic acid bacteria.
28797225	9	118	theme	mutant	1491:1496	arg1	strain					1498:1503	The R:ΔpolE mutant strain	1479:1503	The R:ΔpolE mutant strain	1479:1503	The R:ΔpolE mutant strain was not able to form a regular pellicle anymore, but secreted an EPS into the medium, which exhibited a similar sugar monomer composition as the pellicle polysaccharide isolated from the R strain.
28797225	9	118	theme	mutant	1491:1496	arg1	able					1513:1516	able	1513:1516	able	1513:1516	The R:ΔpolE mutant strain was not able to form a regular pellicle anymore, but secreted an EPS into the medium, which exhibited a similar sugar monomer composition as the pellicle polysaccharide isolated from the R strain.
28797225	8	119	theme	morphological	1336:1348	arg1	change					1350:1355	The morphological change	1332:1355	The morphological change from K. baliensis NBRC 16680 S to R strain	1332:1398	The morphological change from K. baliensis NBRC 16680 S to R strain was accompanied by changes in the sugar contents of the produced pellicle EPS.
28797225	0	120	theme	capsular	49:56	arg1	formation					73:81	capsular polysaccharide formation	49:81	capsular polysaccharide formation	49:81	Environmentally triggered genomic plasticity and capsular polysaccharide formation are involved in increased ethanol and acetic acid tolerance in Kozakia baliensis NBRC 16680.
28797225	13	121	theme	polE	2207:2210	arg1	gene					2212:2215	The polE gene	2203:2215	The polE gene	2203:2215	The polE gene turned out to be involved in the formation of a cell-associated, capsular polysaccharide, which seems to be essential for increased ethanol/acetic tolerance in contrast to the secreted gum-cluster derived heteropolysaccharide.
28797225	7	122	theme	colonies	1244:1251	arg1	number					1232:1237	The number	1228:1237	The number of R colonies	1228:1251	RESULTS The number of R colonies was significantly increased upon growth on acetic acid and especially ethanol.
28797225	9	123	theme	regular	1528:1534	arg1	anymore					1545:1551	a regular pellicle anymore	1526:1551	a regular pellicle anymore	1526:1551	The R:ΔpolE mutant strain was not able to form a regular pellicle anymore, but secreted an EPS into the medium, which exhibited a similar sugar monomer composition as the pellicle polysaccharide isolated from the R strain.
28797225	10	124	theme	other	1798:1802	arg1	strains					1815:1821	the other NBRC 16680 strains	1794:1821	the other NBRC 16680 strains (S, R:ΔpolE)	1794:1834	The R strain had a markedly increased tolerance towards acetic acid and ethanol compared to the other NBRC 16680 strains (S, R:ΔpolE).
28797225	10	124	theme	other	1798:1802	arg1	S					1824:1824	S	1824:1824	S	1824:1824	The R strain had a markedly increased tolerance towards acetic acid and ethanol compared to the other NBRC 16680 strains (S, R:ΔpolE).
28797225	5	125	from	acid	944:947	arg1	experiments					978:988	static growth experiments	964:988	static growth experiments	964:988	Furthermore, we investigated the tolerance of R and S strains against ethanol and acetic acid in shaking and static growth experiments.
28797225	5	125	from	acid	944:947	arg1	shaking					952:958	shaking	952:958	shaking	952:958	Furthermore, we investigated the tolerance of R and S strains against ethanol and acetic acid in shaking and static growth experiments.
28797225	8	126	theme	R	1391:1391	arg1	strain					1393:1398	R strain	1391:1398	R strain	1391:1398	The morphological change from K. baliensis NBRC 16680 S to R strain was accompanied by changes in the sugar contents of the produced pellicle EPS.
28797225	6	127	theme	R	1098:1098	arg1	strain					1100:1105	the R strain	1094:1105	the R strain	1094:1105	To get new insights into the composition and function of the pellicle polysaccharide, the polE gene of the R strain was additionally deleted, as it was reported to be involved in pellicle formation in other acetic acid bacteria.
28797225	7	128	from	growth	1286:1291	arg1	acid					1303:1306	acetic acid	1296:1306	acetic acid	1296:1306	RESULTS The number of R colonies was significantly increased upon growth on acetic acid and especially ethanol.
28797225	7	128	from	growth	1286:1291	arg1	ethanol					1323:1329	ethanol	1323:1329	ethanol	1323:1329	RESULTS The number of R colonies was significantly increased upon growth on acetic acid and especially ethanol.
28797225	7	129	theme	acetic	1296:1301	arg1	acid					1303:1306	acetic acid	1296:1306	acetic acid	1296:1306	RESULTS The number of R colonies was significantly increased upon growth on acetic acid and especially ethanol.
28797225	4	130	theme	growth	786:791	arg1	ethanol					814:820	ethanol	814:820	ethanol	814:820	To elucidate, whether this is a directed switch triggered by environmental factors, we checked the number of R and S colonies under different growth conditions including ethanol and acetic acid supplementation.
28797225	4	130	theme	growth	786:791	arg1	conditions					793:802	different growth conditions	776:802	different growth conditions including ethanol and acetic acid supplementation	776:852	To elucidate, whether this is a directed switch triggered by environmental factors, we checked the number of R and S colonies under different growth conditions including ethanol and acetic acid supplementation.
28797225	4	130	theme	growth	786:791	arg1	supplementation					838:852	acetic acid supplementation	826:852	acetic acid supplementation	826:852	To elucidate, whether this is a directed switch triggered by environmental factors, we checked the number of R and S colonies under different growth conditions including ethanol and acetic acid supplementation.
28797225	2	131	theme	agar	406:409	arg1	plates					411:416	agar plates	406:416	agar plates	406:416	Furthermore, this strain exhibits two colony types on agar plates; smooth wild-type (S) and rough mutant colonies (R).
28627460	12	0	located	observed	2296:2303	arg2	production					2240:2249	increased INF-γ production	2224:2249	increased INF-γ production stimulated by EPS-activated macrophages	2224:2289	Strikingly, CD4 T-cell differentiation and increased INF-γ production stimulated by EPS-activated macrophages were observed in the research.
28627460	12	0	located	observed	2296:2303	arg1	research					2312:2319	the research	2308:2319	the research	2308:2319	Strikingly, CD4 T-cell differentiation and increased INF-γ production stimulated by EPS-activated macrophages were observed in the research.
28627460	12	0	located	observed	2296:2303	arg2	differentiation					2204:2218	CD4 T-cell differentiation	2193:2218	CD4 T-cell differentiation	2193:2218	Strikingly, CD4 T-cell differentiation and increased INF-γ production stimulated by EPS-activated macrophages were observed in the research.
28627460	11	1	theme	Ag	2148:2149	arg1	function					2164:2171	Ag presentation function	2148:2171	maturation as well as Ag presentation function of DCs	2126:2178	Moreover, Our study suggested EPS could remarkably stimulate macrophages to secrete substantial anti-cancer cytokines and promote maturation as well as Ag presentation function of DCs.
28627460	3	2	attach	derived	510:516	arg2	polysaccharides					494:508	polysaccharides	494:508	polysaccharides derived from traditional Chinese medicine	494:550	Accumulating evidence indicated polysaccharides derived from traditional Chinese medicine have potent immune-enhancing properties and relatively nontoxic effects in cancer treatment.
28627460	3	2	attach	derived	510:516	arg1	medicine					543:550	traditional Chinese medicine	523:550	traditional Chinese medicine	523:550	Accumulating evidence indicated polysaccharides derived from traditional Chinese medicine have potent immune-enhancing properties and relatively nontoxic effects in cancer treatment.
28627460	7	3	theme	mice	1393:1396	arg1	model					1398:1402	LLC-bearing mice model	1381:1402	LLC-bearing mice model	1381:1402	In LLC-bearing mice model, we investigated its antitumor activities through evaluation of tumor cell proliferative activity, calculation of immune organ indexes and relative host immune system function tests.
28627460	14	4	theme	experimental	2464:2475	arg1	findings					2477:2484	experimental findings	2464:2484	experimental findings	2464:2484	Taken together, experimental findings suggested EPS could be regarded as a potential immune-stimulating modifier for cancer therapy.
28627460	10	5	from	presence	1904:1911	arg1	EPS					1932:1934	EPS	1932:1934	EPS	1932:1934	FT-IR suggested the presence of pyraoid ring in EPS and SEM displayed smooth surface embedded by several pores.
28627460	10	5	from	presence	1904:1911	arg1	SEM					1940:1942	SEM	1940:1942	SEM	1940:1942	FT-IR suggested the presence of pyraoid ring in EPS and SEM displayed smooth surface embedded by several pores.
28627460	14	6	theme	cancer	2565:2570	arg1	therapy					2572:2578	cancer therapy	2565:2578	cancer therapy	2565:2578	Taken together, experimental findings suggested EPS could be regarded as a potential immune-stimulating modifier for cancer therapy.
28627460	16	7	theme	tumor	2873:2877	arg1	growth					2879:2884	the tumor growth	2869:2884	the tumor growth in LLC-bearing mice	2869:2904	Furthermore, it may inhibit the tumor growth in LLC-bearing mice through regulating host immune system function.
28627460	10	8	dep	suggested	1890:1898	arg1	displayed					1944:1952	displayed	1944:1952	suggested the presence of pyraoid ring in EPS and SEM displayed smooth surface embedded by several pores	1890:1993	FT-IR suggested the presence of pyraoid ring in EPS and SEM displayed smooth surface embedded by several pores.
28627460	15	9	dep	Nakai	2683:2687	arg1	promote					2695:2701	promote	2695:2701	promote maturation and Ag presentation function of DCs	2695:2748	CONCLUSION Our studies demonstrated the polysaccharide (180 × 104Da) purified from Epimedium koreanum Nakai could promote maturation and Ag presentation function of DCs, increase the level of immunomodulatory cytokines and activate CD4 T-cell differentiation.
28627460	15	9	dep	Nakai	2683:2687	arg1	activate					2804:2811	activate	2804:2811	activate CD4 T-cell differentiation	2804:2838	CONCLUSION Our studies demonstrated the polysaccharide (180 × 104Da) purified from Epimedium koreanum Nakai could promote maturation and Ag presentation function of DCs, increase the level of immunomodulatory cytokines and activate CD4 T-cell differentiation.
28627460	15	9	dep	Nakai	2683:2687	arg1	increase					2751:2758	increase	2751:2758	increase the level of immunomodulatory cytokines	2751:2798	CONCLUSION Our studies demonstrated the polysaccharide (180 × 104Da) purified from Epimedium koreanum Nakai could promote maturation and Ag presentation function of DCs, increase the level of immunomodulatory cytokines and activate CD4 T-cell differentiation.
28627460	3	10	theme	nontoxic	607:614	arg1	effects					616:622	relatively nontoxic effects	596:622	relatively nontoxic effects	596:622	Accumulating evidence indicated polysaccharides derived from traditional Chinese medicine have potent immune-enhancing properties and relatively nontoxic effects in cancer treatment.
28627460	15	11	dep	CONCLUSION	2581:2590	arg1	demonstrated					2604:2615	demonstrated	2604:2615	demonstrated the polysaccharide (180 × 104Da) purified from Epimedium koreanum Nakai could promote maturation and Ag presentation function of DCs, increase the level of immunomodulatory cytokines and activate CD4 T-cell differentiation	2604:2838	CONCLUSION Our studies demonstrated the polysaccharide (180 × 104Da) purified from Epimedium koreanum Nakai could promote maturation and Ag presentation function of DCs, increase the level of immunomodulatory cytokines and activate CD4 T-cell differentiation.
28627460	11	12	theme	DCs	2176:2178	arg1	maturation					2126:2135	maturation	2126:2135	maturation as well as Ag presentation function of DCs	2126:2178	Moreover, Our study suggested EPS could remarkably stimulate macrophages to secrete substantial anti-cancer cytokines and promote maturation as well as Ag presentation function of DCs.
28627460	11	12	theme	DCs	2176:2178	arg1	function					2164:2171	Ag presentation function	2148:2171	maturation as well as Ag presentation function of DCs	2126:2178	Moreover, Our study suggested EPS could remarkably stimulate macrophages to secrete substantial anti-cancer cytokines and promote maturation as well as Ag presentation function of DCs.
28627460	6	13	theme	CD4	1311:1313	arg1	differentiation					1322:1336	CD4 T-cell differentiation	1311:1336	CD4 T-cell differentiation	1311:1336	MATERIALS AND METHODS We firstly purified Epimedium koreanum polysaccharide (EPS) from crude extracts and evaluated EPS in vitro using immunological experiments including maturation and Ag presentation function of DCs, CD4 T-cell differentiation and secretion of anti-cancer cytokines.
28627460	4	14	theme	polysaccharides	734:748	arg1	tumor					700:704	tumor	700:704	tumor of Epimedium koreanum Nakai polysaccharides	700:748	However, information about immunological regulation in tumor of Epimedium koreanum Nakai polysaccharides is limited and the reports of purification, characterization of polysaccharides have remained less.
28627460	15	15	theme	presentation	2721:2732	arg1	function					2734:2741	Ag presentation function	2718:2741	Ag presentation function	2718:2741	CONCLUSION Our studies demonstrated the polysaccharide (180 × 104Da) purified from Epimedium koreanum Nakai could promote maturation and Ag presentation function of DCs, increase the level of immunomodulatory cytokines and activate CD4 T-cell differentiation.
28627460	13	16	theme	immune	2404:2409	arg1	system					2411:2416	host immune system	2399:2416	host immune system function in LLC-bearing mice	2399:2445	Furthermore, EPS exhibited prominent antitumor activities through regulating host immune system function in LLC-bearing mice.
28627460	14	17	theme	potential	2523:2531	arg1	modifier					2552:2559	a potential immune-stimulating modifier	2521:2559	a potential immune-stimulating modifier for cancer therapy	2521:2578	Taken together, experimental findings suggested EPS could be regarded as a potential immune-stimulating modifier for cancer therapy.
28627460	6	18	theme	DCs	1306:1308	arg1	maturation					1263:1272	maturation	1263:1272	maturation	1263:1272	MATERIALS AND METHODS We firstly purified Epimedium koreanum polysaccharide (EPS) from crude extracts and evaluated EPS in vitro using immunological experiments including maturation and Ag presentation function of DCs, CD4 T-cell differentiation and secretion of anti-cancer cytokines.
28627460	6	18	theme	DCs	1306:1308	arg1	differentiation					1322:1336	CD4 T-cell differentiation	1311:1336	CD4 T-cell differentiation	1311:1336	MATERIALS AND METHODS We firstly purified Epimedium koreanum polysaccharide (EPS) from crude extracts and evaluated EPS in vitro using immunological experiments including maturation and Ag presentation function of DCs, CD4 T-cell differentiation and secretion of anti-cancer cytokines.
28627460	6	18	theme	DCs	1306:1308	arg1	function					1294:1301	Ag presentation function	1278:1301	Ag presentation function	1278:1301	MATERIALS AND METHODS We firstly purified Epimedium koreanum polysaccharide (EPS) from crude extracts and evaluated EPS in vitro using immunological experiments including maturation and Ag presentation function of DCs, CD4 T-cell differentiation and secretion of anti-cancer cytokines.
28627460	6	18	theme	DCs	1306:1308	arg1	secretion					1342:1350	secretion	1342:1350	secretion	1342:1350	MATERIALS AND METHODS We firstly purified Epimedium koreanum polysaccharide (EPS) from crude extracts and evaluated EPS in vitro using immunological experiments including maturation and Ag presentation function of DCs, CD4 T-cell differentiation and secretion of anti-cancer cytokines.
28627460	11	19	theme	anti-cancer	2092:2102	arg1	cytokines					2104:2112	substantial anti-cancer cytokines	2080:2112	substantial anti-cancer cytokines	2080:2112	Moreover, Our study suggested EPS could remarkably stimulate macrophages to secrete substantial anti-cancer cytokines and promote maturation as well as Ag presentation function of DCs.
28627460	7	20	theme	immune	1557:1562	arg1	system					1564:1569	relative host immune system	1543:1569	relative host immune system	1543:1569	In LLC-bearing mice model, we investigated its antitumor activities through evaluation of tumor cell proliferative activity, calculation of immune organ indexes and relative host immune system function tests.
28627460	0	21	from	effect	92:97	arg1	growth					108:113	tumor growth	102:113	tumor growth	102:113	Polysaccharides from Epimedium koreanum Nakai with immunomodulatory activity and inhibitory effect on tumor growth in LLC-bearing mice.
28627460	0	21	from	effect	92:97	arg1	mice					130:133	LLC-bearing mice	118:133	LLC-bearing mice	118:133	Polysaccharides from Epimedium koreanum Nakai with immunomodulatory activity and inhibitory effect on tumor growth in LLC-bearing mice.
28627460	7	22	theme	relative	1543:1550	arg1	system					1564:1569	relative host immune system	1543:1569	relative host immune system	1543:1569	In LLC-bearing mice model, we investigated its antitumor activities through evaluation of tumor cell proliferative activity, calculation of immune organ indexes and relative host immune system function tests.
28627460	7	23	theme	activity	1493:1500	arg1	calculation					1503:1513	calculation	1503:1513	calculation of immune organ indexes	1503:1537	In LLC-bearing mice model, we investigated its antitumor activities through evaluation of tumor cell proliferative activity, calculation of immune organ indexes and relative host immune system function tests.
28627460	7	23	theme	activity	1493:1500	arg1	evaluation					1454:1463	evaluation	1454:1463	evaluation of tumor cell proliferative activity	1454:1500	In LLC-bearing mice model, we investigated its antitumor activities through evaluation of tumor cell proliferative activity, calculation of immune organ indexes and relative host immune system function tests.
28627460	7	23	theme	activity	1493:1500	arg1	system					1564:1569	relative host immune system	1543:1569	relative host immune system	1543:1569	In LLC-bearing mice model, we investigated its antitumor activities through evaluation of tumor cell proliferative activity, calculation of immune organ indexes and relative host immune system function tests.
28627460	7	24	theme	indexes	1531:1537	arg1	calculation					1503:1513	calculation	1503:1513	calculation of immune organ indexes	1503:1537	In LLC-bearing mice model, we investigated its antitumor activities through evaluation of tumor cell proliferative activity, calculation of immune organ indexes and relative host immune system function tests.
28627460	7	24	theme	indexes	1531:1537	arg1	evaluation					1454:1463	evaluation	1454:1463	evaluation of tumor cell proliferative activity	1454:1500	In LLC-bearing mice model, we investigated its antitumor activities through evaluation of tumor cell proliferative activity, calculation of immune organ indexes and relative host immune system function tests.
28627460	7	24	theme	indexes	1531:1537	arg1	system					1564:1569	relative host immune system	1543:1569	relative host immune system	1543:1569	In LLC-bearing mice model, we investigated its antitumor activities through evaluation of tumor cell proliferative activity, calculation of immune organ indexes and relative host immune system function tests.
28627460	11	25	dep	suggested	2016:2024	arg1	stimulate					2047:2055	stimulate	2047:2055	suggested EPS could remarkably stimulate macrophages to secrete substantial anti-cancer cytokines and promote maturation as well as Ag presentation function of DCs	2016:2178	Moreover, Our study suggested EPS could remarkably stimulate macrophages to secrete substantial anti-cancer cytokines and promote maturation as well as Ag presentation function of DCs.
28627460	8	26	theme	×	1624:1624	arg1	104Da					1626:1630	180 × 104Da	1620:1630	180 × 104Da	1620:1630	RESULTS Results showed that EPS (180 × 104Da) was composed of mannose (Man), rhamnose (Rha), glucuronic acid (GlcUA), galactosamine (GalN), glucose (Glc), galactose (Gal), arabinose (Ara) and fructose (Fuc).
28627460	8	26	theme	×	1624:1624	arg1	EPS					1615:1617	EPS	1615:1617	EPS (180 × 104Da)	1615:1631	RESULTS Results showed that EPS (180 × 104Da) was composed of mannose (Man), rhamnose (Rha), glucuronic acid (GlcUA), galactosamine (GalN), glucose (Glc), galactose (Gal), arabinose (Ara) and fructose (Fuc).
28627460	7	27	theme	immune	1518:1523	arg1	indexes					1531:1537	immune organ indexes	1518:1537	immune organ indexes	1518:1537	In LLC-bearing mice model, we investigated its antitumor activities through evaluation of tumor cell proliferative activity, calculation of immune organ indexes and relative host immune system function tests.
28627460	12	28	theme	EPS-activated	2265:2277	arg1	macrophages					2279:2289	EPS-activated macrophages	2265:2289	EPS-activated macrophages	2265:2289	Strikingly, CD4 T-cell differentiation and increased INF-γ production stimulated by EPS-activated macrophages were observed in the research.
28627460	5	29	theme	reasonable	1040:1049	arg1	explanation					1051:1061	reasonable explanation	1040:1061	reasonable explanation for traditional application	1040:1089	The purpose of our study was to further investigate the active polysaccharides from Epimedium koreanum Nakai by evaluating the immune-regulation activities in tumor-bearing mice and provide reasonable explanation for traditional application.
28627460	10	30	attach	presence	1904:1911	arg1	EPS					1932:1934	EPS	1932:1934	EPS	1932:1934	FT-IR suggested the presence of pyraoid ring in EPS and SEM displayed smooth surface embedded by several pores.
28627460	10	30	attach	presence	1904:1911	arg2	ring					1924:1927	pyraoid ring	1916:1927	pyraoid ring	1916:1927	FT-IR suggested the presence of pyraoid ring in EPS and SEM displayed smooth surface embedded by several pores.
28627460	10	30	attach	presence	1904:1911	arg1	SEM					1940:1942	SEM	1940:1942	SEM	1940:1942	FT-IR suggested the presence of pyraoid ring in EPS and SEM displayed smooth surface embedded by several pores.
28627460	7	31	theme	LLC-bearing	1381:1391	arg1	model					1398:1402	LLC-bearing mice model	1381:1402	LLC-bearing mice model	1381:1402	In LLC-bearing mice model, we investigated its antitumor activities through evaluation of tumor cell proliferative activity, calculation of immune organ indexes and relative host immune system function tests.
28627460	1	32	contain	has	261:263	arg1	RELEVANCE					157:165	ETHNOPHARMACOLOGICAL RELEVANCE Epimedium koreanum Nakai	136:190	ETHNOPHARMACOLOGICAL RELEVANCE Epimedium koreanum Nakai	136:190	ETHNOPHARMACOLOGICAL RELEVANCE Epimedium koreanum Nakai is documented as tonic herbal in China for over a thousand years and has the potential to enhance the body's immunity according to the theory of traditional Chinese medicine.
28627460	1	32	contain	has	261:263	arg2	potential					269:277	the potential to enhance the body's immunity according to the theory of traditional Chinese medicine	265:364	the potential to enhance the body's immunity according to the theory of traditional Chinese medicine	265:364	ETHNOPHARMACOLOGICAL RELEVANCE Epimedium koreanum Nakai is documented as tonic herbal in China for over a thousand years and has the potential to enhance the body's immunity according to the theory of traditional Chinese medicine.
28627460	5	33	from	Nakai	953:957	arg1	polysaccharides					913:927	the active polysaccharides	902:927	the active polysaccharides from Epimedium koreanum Nakai	902:957	The purpose of our study was to further investigate the active polysaccharides from Epimedium koreanum Nakai by evaluating the immune-regulation activities in tumor-bearing mice and provide reasonable explanation for traditional application.
28627460	15	34	theme	immunomodulatory	2773:2788	arg1	cytokines					2790:2798	immunomodulatory cytokines	2773:2798	immunomodulatory cytokines	2773:2798	CONCLUSION Our studies demonstrated the polysaccharide (180 × 104Da) purified from Epimedium koreanum Nakai could promote maturation and Ag presentation function of DCs, increase the level of immunomodulatory cytokines and activate CD4 T-cell differentiation.
28627460	1	35	theme	traditional	337:347	arg1	medicine					357:364	traditional Chinese medicine	337:364	traditional Chinese medicine	337:364	ETHNOPHARMACOLOGICAL RELEVANCE Epimedium koreanum Nakai is documented as tonic herbal in China for over a thousand years and has the potential to enhance the body's immunity according to the theory of traditional Chinese medicine.
28627460	5	36	theme	tumor-bearing	1009:1021	arg1	mice					1023:1026	tumor-bearing mice	1009:1026	tumor-bearing mice	1009:1026	The purpose of our study was to further investigate the active polysaccharides from Epimedium koreanum Nakai by evaluating the immune-regulation activities in tumor-bearing mice and provide reasonable explanation for traditional application.
28627460	9	37	theme	composition	1804:1814	arg1	assay					1816:1820	Chemical composition assay	1795:1820	Chemical composition assay	1795:1820	Chemical composition assay indicated EPS was a fraction with 28.20% uronic acid content.
28627460	0	38	theme	tumor	102:106	arg1	growth					108:113	tumor growth	102:113	tumor growth	102:113	Polysaccharides from Epimedium koreanum Nakai with immunomodulatory activity and inhibitory effect on tumor growth in LLC-bearing mice.
28627460	1	39	theme	medicine	357:364	arg1	theory					327:332	the theory	323:332	the theory of traditional Chinese medicine	323:364	ETHNOPHARMACOLOGICAL RELEVANCE Epimedium koreanum Nakai is documented as tonic herbal in China for over a thousand years and has the potential to enhance the body's immunity according to the theory of traditional Chinese medicine.
28627460	13	40	theme	prominent	2349:2357	arg1	activities					2369:2378	prominent antitumor activities	2349:2378	prominent antitumor activities	2349:2378	Furthermore, EPS exhibited prominent antitumor activities through regulating host immune system function in LLC-bearing mice.
28627460	6	41	theme	crude	1179:1183	arg1	extracts					1185:1192	crude extracts	1179:1192	crude extracts	1179:1192	MATERIALS AND METHODS We firstly purified Epimedium koreanum polysaccharide (EPS) from crude extracts and evaluated EPS in vitro using immunological experiments including maturation and Ag presentation function of DCs, CD4 T-cell differentiation and secretion of anti-cancer cytokines.
28627460	13	42	theme	LLC-bearing	2430:2440	arg1	mice					2442:2445	LLC-bearing mice	2430:2445	LLC-bearing mice	2430:2445	Furthermore, EPS exhibited prominent antitumor activities through regulating host immune system function in LLC-bearing mice.
28627460	0	43	from	Nakai	40:44	arg1	Polysaccharides					0:14	Polysaccharides	0:14	Polysaccharides from Epimedium koreanum Nakai with immunomodulatory activity and inhibitory effect on tumor growth in LLC-bearing mice.	0:134	Polysaccharides from Epimedium koreanum Nakai with immunomodulatory activity and inhibitory effect on tumor growth in LLC-bearing mice.
28627460	15	44	theme	cytokines	2790:2798	arg1	level					2764:2768	the level	2760:2768	the level of immunomodulatory cytokines	2760:2798	CONCLUSION Our studies demonstrated the polysaccharide (180 × 104Da) purified from Epimedium koreanum Nakai could promote maturation and Ag presentation function of DCs, increase the level of immunomodulatory cytokines and activate CD4 T-cell differentiation.
28627460	10	45	theme	smooth	1954:1959	arg1	surface					1961:1967	smooth surface	1954:1967	smooth surface embedded by several pores	1954:1993	FT-IR suggested the presence of pyraoid ring in EPS and SEM displayed smooth surface embedded by several pores.
28627460	7	46	theme	proliferative	1479:1491	arg1	activity					1493:1500	tumor cell proliferative activity	1468:1500	tumor cell proliferative activity	1468:1500	In LLC-bearing mice model, we investigated its antitumor activities through evaluation of tumor cell proliferative activity, calculation of immune organ indexes and relative host immune system function tests.
28627460	6	47	dep	in	1212:1213	arg1	vitro					1215:1219	vitro	1215:1219	vitro	1215:1219	MATERIALS AND METHODS We firstly purified Epimedium koreanum polysaccharide (EPS) from crude extracts and evaluated EPS in vitro using immunological experiments including maturation and Ag presentation function of DCs, CD4 T-cell differentiation and secretion of anti-cancer cytokines.
28627460	5	48	theme	active	906:911	arg1	polysaccharides					913:927	the active polysaccharides	902:927	the active polysaccharides from Epimedium koreanum Nakai	902:957	The purpose of our study was to further investigate the active polysaccharides from Epimedium koreanum Nakai by evaluating the immune-regulation activities in tumor-bearing mice and provide reasonable explanation for traditional application.
28627460	16	49	theme	immune	2930:2935	arg1	function					2944:2951	host immune system function	2925:2951	host immune system function	2925:2951	Furthermore, it may inhibit the tumor growth in LLC-bearing mice through regulating host immune system function.
28627460	7	50	theme	tumor	1468:1472	arg1	activity					1493:1500	tumor cell proliferative activity	1468:1500	tumor cell proliferative activity	1468:1500	In LLC-bearing mice model, we investigated its antitumor activities through evaluation of tumor cell proliferative activity, calculation of immune organ indexes and relative host immune system function tests.
28627460	9	51	theme	28.20	1856:1860	arg1	%					1861:1861	%	1861:1861	%	1861:1861	Chemical composition assay indicated EPS was a fraction with 28.20% uronic acid content.
28627460	15	52	theme	DCs	2746:2748	arg1	function					2734:2741	Ag presentation function	2718:2741	Ag presentation function	2718:2741	CONCLUSION Our studies demonstrated the polysaccharide (180 × 104Da) purified from Epimedium koreanum Nakai could promote maturation and Ag presentation function of DCs, increase the level of immunomodulatory cytokines and activate CD4 T-cell differentiation.
28627460	15	52	theme	DCs	2746:2748	arg1	maturation					2703:2712	maturation	2703:2712	maturation	2703:2712	CONCLUSION Our studies demonstrated the polysaccharide (180 × 104Da) purified from Epimedium koreanum Nakai could promote maturation and Ag presentation function of DCs, increase the level of immunomodulatory cytokines and activate CD4 T-cell differentiation.
28627460	3	53	theme	Accumulating	462:473	arg1	evidence					475:482	Accumulating evidence	462:482	Accumulating evidence	462:482	Accumulating evidence indicated polysaccharides derived from traditional Chinese medicine have potent immune-enhancing properties and relatively nontoxic effects in cancer treatment.
28627460	2	54	theme	effective	413:421	arg1	compounds					423:431	the most important effective compounds	394:431	the most important effective compounds	394:431	Polysaccharides are one of the most important effective compounds in Epimedium koreanum Nakai.
28627460	5	55	theme	study	869:873	arg1	purpose					854:860	The purpose	850:860	The purpose of our study	850:873	The purpose of our study was to further investigate the active polysaccharides from Epimedium koreanum Nakai by evaluating the immune-regulation activities in tumor-bearing mice and provide reasonable explanation for traditional application.
28627460	9	56	theme	uronic	1863:1868	arg1	content					1875:1881	28.20% uronic acid content	1856:1881	28.20% uronic acid content	1856:1881	Chemical composition assay indicated EPS was a fraction with 28.20% uronic acid content.
28627460	8	57	theme	glucuronic	1680:1689	arg1	acid					1691:1694	glucuronic acid	1680:1694	glucuronic acid (GlcUA)	1680:1702	RESULTS Results showed that EPS (180 × 104Da) was composed of mannose (Man), rhamnose (Rha), glucuronic acid (GlcUA), galactosamine (GalN), glucose (Glc), galactose (Gal), arabinose (Ara) and fructose (Fuc).
28627460	8	57	theme	glucuronic	1680:1689	arg1	GlcUA					1697:1701	GlcUA	1697:1701	GlcUA	1697:1701	RESULTS Results showed that EPS (180 × 104Da) was composed of mannose (Man), rhamnose (Rha), glucuronic acid (GlcUA), galactosamine (GalN), glucose (Glc), galactose (Gal), arabinose (Ara) and fructose (Fuc).
28627460	4	58	from	regulation	686:695	arg1	tumor					700:704	tumor	700:704	tumor of Epimedium koreanum Nakai polysaccharides	700:748	However, information about immunological regulation in tumor of Epimedium koreanum Nakai polysaccharides is limited and the reports of purification, characterization of polysaccharides have remained less.
28627460	12	59	theme	INF-γ	2234:2238	arg1	production					2240:2249	increased INF-γ production	2224:2249	increased INF-γ production stimulated by EPS-activated macrophages	2224:2289	Strikingly, CD4 T-cell differentiation and increased INF-γ production stimulated by EPS-activated macrophages were observed in the research.
28627460	3	60	theme	traditional	523:533	arg1	medicine					543:550	traditional Chinese medicine	523:550	traditional Chinese medicine	523:550	Accumulating evidence indicated polysaccharides derived from traditional Chinese medicine have potent immune-enhancing properties and relatively nontoxic effects in cancer treatment.
28627460	0	61	theme	immunomodulatory	51:66	arg1	activity					68:75	immunomodulatory activity	51:75	immunomodulatory activity	51:75	Polysaccharides from Epimedium koreanum Nakai with immunomodulatory activity and inhibitory effect on tumor growth in LLC-bearing mice.
28627460	8	62	theme	RESULTS	1587:1593	arg1	Results					1595:1601	RESULTS Results	1587:1601	RESULTS Results	1587:1601	RESULTS Results showed that EPS (180 × 104Da) was composed of mannose (Man), rhamnose (Rha), glucuronic acid (GlcUA), galactosamine (GalN), glucose (Glc), galactose (Gal), arabinose (Ara) and fructose (Fuc).
28627460	0	63	with	Polysaccharides	0:14	arg1	effect					92:97	inhibitory effect	81:97	inhibitory effect on tumor growth in LLC-bearing mice	81:133	Polysaccharides from Epimedium koreanum Nakai with immunomodulatory activity and inhibitory effect on tumor growth in LLC-bearing mice.
28627460	0	63	with	Polysaccharides	0:14	arg1	activity					68:75	immunomodulatory activity	51:75	immunomodulatory activity	51:75	Polysaccharides from Epimedium koreanum Nakai with immunomodulatory activity and inhibitory effect on tumor growth in LLC-bearing mice.
28627460	7	64	dep	evaluation	1454:1463	arg1	tests					1580:1584	function tests	1571:1584	function tests	1571:1584	In LLC-bearing mice model, we investigated its antitumor activities through evaluation of tumor cell proliferative activity, calculation of immune organ indexes and relative host immune system function tests.
28627460	12	65	theme	T-cell	2197:2202	arg1	differentiation					2204:2218	CD4 T-cell differentiation	2193:2218	CD4 T-cell differentiation	2193:2218	Strikingly, CD4 T-cell differentiation and increased INF-γ production stimulated by EPS-activated macrophages were observed in the research.
28627460	13	66	from	function	2418:2425	arg1	mice					2442:2445	LLC-bearing mice	2430:2445	LLC-bearing mice	2430:2445	Furthermore, EPS exhibited prominent antitumor activities through regulating host immune system function in LLC-bearing mice.
28627460	3	67	theme	potent	557:562	arg1	properties					581:590	potent immune-enhancing properties	557:590	potent immune-enhancing properties	557:590	Accumulating evidence indicated polysaccharides derived from traditional Chinese medicine have potent immune-enhancing properties and relatively nontoxic effects in cancer treatment.
28627460	6	68	theme	immunological	1227:1239	arg1	maturation					1263:1272	maturation	1263:1272	maturation	1263:1272	MATERIALS AND METHODS We firstly purified Epimedium koreanum polysaccharide (EPS) from crude extracts and evaluated EPS in vitro using immunological experiments including maturation and Ag presentation function of DCs, CD4 T-cell differentiation and secretion of anti-cancer cytokines.
28627460	6	68	theme	immunological	1227:1239	arg1	experiments					1241:1251	immunological experiments	1227:1251	immunological experiments including maturation and Ag presentation function of DCs, CD4 T-cell differentiation and secretion of anti-cancer cytokines	1227:1375	MATERIALS AND METHODS We firstly purified Epimedium koreanum polysaccharide (EPS) from crude extracts and evaluated EPS in vitro using immunological experiments including maturation and Ag presentation function of DCs, CD4 T-cell differentiation and secretion of anti-cancer cytokines.
28627460	6	68	theme	immunological	1227:1239	arg1	secretion					1342:1350	secretion	1342:1350	secretion	1342:1350	MATERIALS AND METHODS We firstly purified Epimedium koreanum polysaccharide (EPS) from crude extracts and evaluated EPS in vitro using immunological experiments including maturation and Ag presentation function of DCs, CD4 T-cell differentiation and secretion of anti-cancer cytokines.
28627460	6	68	theme	immunological	1227:1239	arg1	function					1294:1301	Ag presentation function	1278:1301	Ag presentation function	1278:1301	MATERIALS AND METHODS We firstly purified Epimedium koreanum polysaccharide (EPS) from crude extracts and evaluated EPS in vitro using immunological experiments including maturation and Ag presentation function of DCs, CD4 T-cell differentiation and secretion of anti-cancer cytokines.
28627460	6	68	theme	immunological	1227:1239	arg1	differentiation					1322:1336	CD4 T-cell differentiation	1311:1336	CD4 T-cell differentiation	1311:1336	MATERIALS AND METHODS We firstly purified Epimedium koreanum polysaccharide (EPS) from crude extracts and evaluated EPS in vitro using immunological experiments including maturation and Ag presentation function of DCs, CD4 T-cell differentiation and secretion of anti-cancer cytokines.
28627460	11	69	theme	presentation	2151:2162	arg1	function					2164:2171	Ag presentation function	2148:2171	maturation as well as Ag presentation function of DCs	2126:2178	Moreover, Our study suggested EPS could remarkably stimulate macrophages to secrete substantial anti-cancer cytokines and promote maturation as well as Ag presentation function of DCs.
28627460	10	70	theme	ring	1924:1927	arg1	presence					1904:1911	the presence	1900:1911	the presence of pyraoid ring in EPS and SEM	1900:1942	FT-IR suggested the presence of pyraoid ring in EPS and SEM displayed smooth surface embedded by several pores.
28627460	7	71	theme	function	1571:1578	arg1	tests					1580:1584	function tests	1571:1584	function tests	1571:1584	In LLC-bearing mice model, we investigated its antitumor activities through evaluation of tumor cell proliferative activity, calculation of immune organ indexes and relative host immune system function tests.
28627460	6	72	theme	T-cell	1315:1320	arg1	differentiation					1322:1336	CD4 T-cell differentiation	1311:1336	CD4 T-cell differentiation	1311:1336	MATERIALS AND METHODS We firstly purified Epimedium koreanum polysaccharide (EPS) from crude extracts and evaluated EPS in vitro using immunological experiments including maturation and Ag presentation function of DCs, CD4 T-cell differentiation and secretion of anti-cancer cytokines.
28627460	7	73	theme	antitumor	1425:1433	arg1	activities					1435:1444	its antitumor activities	1421:1444	its antitumor activities	1421:1444	In LLC-bearing mice model, we investigated its antitumor activities through evaluation of tumor cell proliferative activity, calculation of immune organ indexes and relative host immune system function tests.
28627460	13	74	theme	system	2411:2416	arg1	function					2418:2425	host immune system function	2399:2425	host immune system function in LLC-bearing mice	2399:2445	Furthermore, EPS exhibited prominent antitumor activities through regulating host immune system function in LLC-bearing mice.
28627460	13	75	theme	host	2399:2402	arg1	system					2411:2416	host immune system	2399:2416	host immune system function in LLC-bearing mice	2399:2445	Furthermore, EPS exhibited prominent antitumor activities through regulating host immune system function in LLC-bearing mice.
28627460	15	76	theme	CD4	2813:2815	arg1	differentiation					2824:2838	CD4 T-cell differentiation	2813:2838	CD4 T-cell differentiation	2813:2838	CONCLUSION Our studies demonstrated the polysaccharide (180 × 104Da) purified from Epimedium koreanum Nakai could promote maturation and Ag presentation function of DCs, increase the level of immunomodulatory cytokines and activate CD4 T-cell differentiation.
28627460	14	77	theme	immune-stimulating	2533:2550	arg1	modifier					2552:2559	a potential immune-stimulating modifier	2521:2559	a potential immune-stimulating modifier for cancer therapy	2521:2578	Taken together, experimental findings suggested EPS could be regarded as a potential immune-stimulating modifier for cancer therapy.
28627460	16	78	theme	host	2925:2928	arg1	function					2944:2951	host immune system function	2925:2951	host immune system function	2925:2951	Furthermore, it may inhibit the tumor growth in LLC-bearing mice through regulating host immune system function.
28627460	4	79	theme	Nakai	728:732	arg1	polysaccharides					734:748	Epimedium koreanum Nakai polysaccharides	709:748	Epimedium koreanum Nakai polysaccharides	709:748	However, information about immunological regulation in tumor of Epimedium koreanum Nakai polysaccharides is limited and the reports of purification, characterization of polysaccharides have remained less.
28627460	3	80	theme	cancer	627:632	arg1	treatment					634:642	cancer treatment	627:642	cancer treatment	627:642	Accumulating evidence indicated polysaccharides derived from traditional Chinese medicine have potent immune-enhancing properties and relatively nontoxic effects in cancer treatment.
28627460	1	81	theme	tonic	209:213	arg1	herbal					215:220	tonic herbal	209:220	tonic herbal	209:220	ETHNOPHARMACOLOGICAL RELEVANCE Epimedium koreanum Nakai is documented as tonic herbal in China for over a thousand years and has the potential to enhance the body's immunity according to the theory of traditional Chinese medicine.
28627460	16	82	theme	system	2937:2942	arg1	function					2944:2951	host immune system function	2925:2951	host immune system function	2925:2951	Furthermore, it may inhibit the tumor growth in LLC-bearing mice through regulating host immune system function.
28627460	10	83	theme	several	1981:1987	arg1	pores					1989:1993	several pores	1981:1993	several pores	1981:1993	FT-IR suggested the presence of pyraoid ring in EPS and SEM displayed smooth surface embedded by several pores.
28627460	15	84	theme	Ag	2718:2719	arg1	function					2734:2741	Ag presentation function	2718:2741	Ag presentation function	2718:2741	CONCLUSION Our studies demonstrated the polysaccharide (180 × 104Da) purified from Epimedium koreanum Nakai could promote maturation and Ag presentation function of DCs, increase the level of immunomodulatory cytokines and activate CD4 T-cell differentiation.
28627460	13	85	theme	antitumor	2359:2367	arg1	activities					2369:2378	prominent antitumor activities	2349:2378	prominent antitumor activities	2349:2378	Furthermore, EPS exhibited prominent antitumor activities through regulating host immune system function in LLC-bearing mice.
28627460	6	86	theme	presentation	1281:1292	arg1	function					1294:1301	Ag presentation function	1278:1301	Ag presentation function	1278:1301	MATERIALS AND METHODS We firstly purified Epimedium koreanum polysaccharide (EPS) from crude extracts and evaluated EPS in vitro using immunological experiments including maturation and Ag presentation function of DCs, CD4 T-cell differentiation and secretion of anti-cancer cytokines.
28627460	16	87	from	growth	2879:2884	arg1	mice					2901:2904	LLC-bearing mice	2889:2904	LLC-bearing mice	2889:2904	Furthermore, it may inhibit the tumor growth in LLC-bearing mice through regulating host immune system function.
28627460	11	88	theme	substantial	2080:2090	arg1	cytokines					2104:2112	substantial anti-cancer cytokines	2080:2112	substantial anti-cancer cytokines	2080:2112	Moreover, Our study suggested EPS could remarkably stimulate macrophages to secrete substantial anti-cancer cytokines and promote maturation as well as Ag presentation function of DCs.
28627460	7	89	theme	host	1552:1555	arg1	system					1564:1569	relative host immune system	1543:1569	relative host immune system	1543:1569	In LLC-bearing mice model, we investigated its antitumor activities through evaluation of tumor cell proliferative activity, calculation of immune organ indexes and relative host immune system function tests.
28627460	14	90	dep	suggested	2486:2494	arg1	regarded					2509:2516	regarded	2509:2516	suggested EPS could be regarded as a potential immune-stimulating modifier for cancer therapy	2486:2578	Taken together, experimental findings suggested EPS could be regarded as a potential immune-stimulating modifier for cancer therapy.
28627460	9	91	with	fraction	1842:1849	arg1	content					1875:1881	28.20% uronic acid content	1856:1881	28.20% uronic acid content	1856:1881	Chemical composition assay indicated EPS was a fraction with 28.20% uronic acid content.
28627460	9	92	dep	indicated	1822:1830	arg1	fraction					1842:1849	a fraction	1840:1849	a fraction with 28.20% uronic acid content	1840:1881	Chemical composition assay indicated EPS was a fraction with 28.20% uronic acid content.
28627460	6	93	dep	MATERIALS	1092:1100	arg1	We					1114:1115	We	1114:1115	We	1114:1115	MATERIALS AND METHODS We firstly purified Epimedium koreanum polysaccharide (EPS) from crude extracts and evaluated EPS in vitro using immunological experiments including maturation and Ag presentation function of DCs, CD4 T-cell differentiation and secretion of anti-cancer cytokines.
28627460	6	94	theme	cytokines	1367:1375	arg1	maturation					1263:1272	maturation	1263:1272	maturation	1263:1272	MATERIALS AND METHODS We firstly purified Epimedium koreanum polysaccharide (EPS) from crude extracts and evaluated EPS in vitro using immunological experiments including maturation and Ag presentation function of DCs, CD4 T-cell differentiation and secretion of anti-cancer cytokines.
28627460	6	94	theme	cytokines	1367:1375	arg1	differentiation					1322:1336	CD4 T-cell differentiation	1311:1336	CD4 T-cell differentiation	1311:1336	MATERIALS AND METHODS We firstly purified Epimedium koreanum polysaccharide (EPS) from crude extracts and evaluated EPS in vitro using immunological experiments including maturation and Ag presentation function of DCs, CD4 T-cell differentiation and secretion of anti-cancer cytokines.
28627460	6	94	theme	cytokines	1367:1375	arg1	function					1294:1301	Ag presentation function	1278:1301	Ag presentation function	1278:1301	MATERIALS AND METHODS We firstly purified Epimedium koreanum polysaccharide (EPS) from crude extracts and evaluated EPS in vitro using immunological experiments including maturation and Ag presentation function of DCs, CD4 T-cell differentiation and secretion of anti-cancer cytokines.
28627460	6	94	theme	cytokines	1367:1375	arg1	secretion					1342:1350	secretion	1342:1350	secretion	1342:1350	MATERIALS AND METHODS We firstly purified Epimedium koreanum polysaccharide (EPS) from crude extracts and evaluated EPS in vitro using immunological experiments including maturation and Ag presentation function of DCs, CD4 T-cell differentiation and secretion of anti-cancer cytokines.
28627460	4	95	theme	purification	780:791	arg1	reports					769:775	the reports	765:775	the reports of purification, characterization of polysaccharides	765:828	However, information about immunological regulation in tumor of Epimedium koreanum Nakai polysaccharides is limited and the reports of purification, characterization of polysaccharides have remained less.
28627460	1	96	theme	ETHNOPHARMACOLOGICAL	136:155	arg1	RELEVANCE					157:165	ETHNOPHARMACOLOGICAL RELEVANCE Epimedium koreanum Nakai	136:190	ETHNOPHARMACOLOGICAL RELEVANCE Epimedium koreanum Nakai	136:190	ETHNOPHARMACOLOGICAL RELEVANCE Epimedium koreanum Nakai is documented as tonic herbal in China for over a thousand years and has the potential to enhance the body's immunity according to the theory of traditional Chinese medicine.
28627460	7	97	theme	organ	1525:1529	arg1	indexes					1531:1537	immune organ indexes	1518:1537	immune organ indexes	1518:1537	In LLC-bearing mice model, we investigated its antitumor activities through evaluation of tumor cell proliferative activity, calculation of immune organ indexes and relative host immune system function tests.
28627460	0	98	theme	inhibitory	81:90	arg1	effect					92:97	inhibitory effect	81:97	inhibitory effect on tumor growth in LLC-bearing mice	81:133	Polysaccharides from Epimedium koreanum Nakai with immunomodulatory activity and inhibitory effect on tumor growth in LLC-bearing mice.
28627460	5	99	theme	immune-regulation	977:993	arg1	activities					995:1004	the immune-regulation activities	973:1004	the immune-regulation activities in tumor-bearing mice	973:1026	The purpose of our study was to further investigate the active polysaccharides from Epimedium koreanum Nakai by evaluating the immune-regulation activities in tumor-bearing mice and provide reasonable explanation for traditional application.
28627460	3	100	contain	have	552:555	arg2	properties					581:590	potent immune-enhancing properties	557:590	potent immune-enhancing properties	557:590	Accumulating evidence indicated polysaccharides derived from traditional Chinese medicine have potent immune-enhancing properties and relatively nontoxic effects in cancer treatment.
28627460	3	100	contain	have	552:555	arg2	effects					616:622	relatively nontoxic effects	596:622	relatively nontoxic effects	596:622	Accumulating evidence indicated polysaccharides derived from traditional Chinese medicine have potent immune-enhancing properties and relatively nontoxic effects in cancer treatment.
28627460	3	100	contain	have	552:555	arg1	polysaccharides					494:508	polysaccharides	494:508	polysaccharides derived from traditional Chinese medicine	494:550	Accumulating evidence indicated polysaccharides derived from traditional Chinese medicine have potent immune-enhancing properties and relatively nontoxic effects in cancer treatment.
28627460	16	101	theme	LLC-bearing	2889:2899	arg1	mice					2901:2904	LLC-bearing mice	2889:2904	LLC-bearing mice	2889:2904	Furthermore, it may inhibit the tumor growth in LLC-bearing mice through regulating host immune system function.
28627460	6	102	theme	Epimedium	1134:1142	arg1	polysaccharide					1153:1166	Epimedium koreanum polysaccharide	1134:1166	Epimedium koreanum polysaccharide (EPS)	1134:1172	MATERIALS AND METHODS We firstly purified Epimedium koreanum polysaccharide (EPS) from crude extracts and evaluated EPS in vitro using immunological experiments including maturation and Ag presentation function of DCs, CD4 T-cell differentiation and secretion of anti-cancer cytokines.
28627460	6	102	theme	Epimedium	1134:1142	arg1	EPS					1169:1171	EPS	1169:1171	EPS	1169:1171	MATERIALS AND METHODS We firstly purified Epimedium koreanum polysaccharide (EPS) from crude extracts and evaluated EPS in vitro using immunological experiments including maturation and Ag presentation function of DCs, CD4 T-cell differentiation and secretion of anti-cancer cytokines.
28627460	9	103	theme	Chemical	1795:1802	arg1	composition					1804:1814	Chemical composition	1795:1814	Chemical composition assay	1795:1820	Chemical composition assay indicated EPS was a fraction with 28.20% uronic acid content.
28627460	6	104	dep	Epimedium	1134:1142	arg1	koreanum					1144:1151	koreanum	1144:1151	koreanum	1144:1151	MATERIALS AND METHODS We firstly purified Epimedium koreanum polysaccharide (EPS) from crude extracts and evaluated EPS in vitro using immunological experiments including maturation and Ag presentation function of DCs, CD4 T-cell differentiation and secretion of anti-cancer cytokines.
28627460	1	105	theme	Chinese	349:355	arg1	medicine					357:364	traditional Chinese medicine	337:364	traditional Chinese medicine	337:364	ETHNOPHARMACOLOGICAL RELEVANCE Epimedium koreanum Nakai is documented as tonic herbal in China for over a thousand years and has the potential to enhance the body's immunity according to the theory of traditional Chinese medicine.
28627460	15	106	theme	×	2641:2641	arg1	104Da					2643:2647	180 × 104Da	2637:2647	180 × 104Da	2637:2647	CONCLUSION Our studies demonstrated the polysaccharide (180 × 104Da) purified from Epimedium koreanum Nakai could promote maturation and Ag presentation function of DCs, increase the level of immunomodulatory cytokines and activate CD4 T-cell differentiation.
28627460	15	106	theme	×	2641:2641	arg1	polysaccharide					2621:2634	the polysaccharide	2617:2634	the polysaccharide (180 × 104Da) purified from Epimedium koreanum Nakai could promote maturation and Ag presentation function of DCs, increase the level of immunomodulatory cytokines and activate CD4 T-cell differentiation	2617:2838	CONCLUSION Our studies demonstrated the polysaccharide (180 × 104Da) purified from Epimedium koreanum Nakai could promote maturation and Ag presentation function of DCs, increase the level of immunomodulatory cytokines and activate CD4 T-cell differentiation.
28627460	0	107	from	activity	68:75	arg1	growth					108:113	tumor growth	102:113	tumor growth	102:113	Polysaccharides from Epimedium koreanum Nakai with immunomodulatory activity and inhibitory effect on tumor growth in LLC-bearing mice.
28627460	0	107	from	activity	68:75	arg1	mice					130:133	LLC-bearing mice	118:133	LLC-bearing mice	118:133	Polysaccharides from Epimedium koreanum Nakai with immunomodulatory activity and inhibitory effect on tumor growth in LLC-bearing mice.
28627460	1	108	dep	RELEVANCE	157:165	arg1	Nakai					186:190	Nakai	186:190	Nakai	186:190	ETHNOPHARMACOLOGICAL RELEVANCE Epimedium koreanum Nakai is documented as tonic herbal in China for over a thousand years and has the potential to enhance the body's immunity according to the theory of traditional Chinese medicine.
28627460	0	109	theme	LLC-bearing	118:128	arg1	mice					130:133	LLC-bearing mice	118:133	LLC-bearing mice	118:133	Polysaccharides from Epimedium koreanum Nakai with immunomodulatory activity and inhibitory effect on tumor growth in LLC-bearing mice.
28627460	5	110	from	activities	995:1004	arg1	mice					1023:1026	tumor-bearing mice	1009:1026	tumor-bearing mice	1009:1026	The purpose of our study was to further investigate the active polysaccharides from Epimedium koreanum Nakai by evaluating the immune-regulation activities in tumor-bearing mice and provide reasonable explanation for traditional application.
28627460	6	111	theme	in	1212:1213	arg1	EPS					1208:1210	EPS	1208:1210	EPS in vitro	1208:1219	MATERIALS AND METHODS We firstly purified Epimedium koreanum polysaccharide (EPS) from crude extracts and evaluated EPS in vitro using immunological experiments including maturation and Ag presentation function of DCs, CD4 T-cell differentiation and secretion of anti-cancer cytokines.
28627460	7	112	theme	cell	1474:1477	arg1	activity					1493:1500	tumor cell proliferative activity	1468:1500	tumor cell proliferative activity	1468:1500	In LLC-bearing mice model, we investigated its antitumor activities through evaluation of tumor cell proliferative activity, calculation of immune organ indexes and relative host immune system function tests.
28627460	4	113	theme	polysaccharides	814:828	arg1	characterization					794:809	characterization	794:809	characterization of polysaccharides	794:828	However, information about immunological regulation in tumor of Epimedium koreanum Nakai polysaccharides is limited and the reports of purification, characterization of polysaccharides have remained less.
28627460	4	113	theme	polysaccharides	814:828	arg1	purification					780:791	purification	780:791	purification	780:791	However, information about immunological regulation in tumor of Epimedium koreanum Nakai polysaccharides is limited and the reports of purification, characterization of polysaccharides have remained less.
28627460	5	114	theme	traditional	1067:1077	arg1	application					1079:1089	traditional application	1067:1089	traditional application	1067:1089	The purpose of our study was to further investigate the active polysaccharides from Epimedium koreanum Nakai by evaluating the immune-regulation activities in tumor-bearing mice and provide reasonable explanation for traditional application.
28627460	10	115	dep	surface	1961:1967	arg1	embedded					1969:1976	embedded	1969:1976	embedded	1969:1976	FT-IR suggested the presence of pyraoid ring in EPS and SEM displayed smooth surface embedded by several pores.
28627460	2	116	theme	compounds	423:431	arg1	one					387:389	one	387:389	one	387:389	Polysaccharides are one of the most important effective compounds in Epimedium koreanum Nakai.
28627460	2	116	theme	compounds	423:431	arg1	compounds					423:431	the most important effective compounds	394:431	the most important effective compounds	394:431	Polysaccharides are one of the most important effective compounds in Epimedium koreanum Nakai.
28627460	15	117	theme	T-cell	2817:2822	arg1	differentiation					2824:2838	CD4 T-cell differentiation	2813:2838	CD4 T-cell differentiation	2813:2838	CONCLUSION Our studies demonstrated the polysaccharide (180 × 104Da) purified from Epimedium koreanum Nakai could promote maturation and Ag presentation function of DCs, increase the level of immunomodulatory cytokines and activate CD4 T-cell differentiation.
28627460	9	118	theme	%	1861:1861	arg1	content					1875:1881	28.20% uronic acid content	1856:1881	28.20% uronic acid content	1856:1881	Chemical composition assay indicated EPS was a fraction with 28.20% uronic acid content.
28627460	2	119	theme	important	403:411	arg1	compounds					423:431	the most important effective compounds	394:431	the most important effective compounds	394:431	Polysaccharides are one of the most important effective compounds in Epimedium koreanum Nakai.
28627460	2	120	from	one	387:389	arg1	Nakai					455:459	Nakai	455:459	Nakai	455:459	Polysaccharides are one of the most important effective compounds in Epimedium koreanum Nakai.
28627460	6	121	theme	Ag	1278:1279	arg1	function					1294:1301	Ag presentation function	1278:1301	Ag presentation function	1278:1301	MATERIALS AND METHODS We firstly purified Epimedium koreanum polysaccharide (EPS) from crude extracts and evaluated EPS in vitro using immunological experiments including maturation and Ag presentation function of DCs, CD4 T-cell differentiation and secretion of anti-cancer cytokines.
28627460	9	122	theme	acid	1870:1873	arg1	content					1875:1881	28.20% uronic acid content	1856:1881	28.20% uronic acid content	1856:1881	Chemical composition assay indicated EPS was a fraction with 28.20% uronic acid content.
28627460	10	123	theme	pyraoid	1916:1922	arg1	ring					1924:1927	pyraoid ring	1916:1927	pyraoid ring	1916:1927	FT-IR suggested the presence of pyraoid ring in EPS and SEM displayed smooth surface embedded by several pores.
28627460	12	124	theme	increased	2224:2232	arg1	production					2240:2249	increased INF-γ production	2224:2249	increased INF-γ production stimulated by EPS-activated macrophages	2224:2289	Strikingly, CD4 T-cell differentiation and increased INF-γ production stimulated by EPS-activated macrophages were observed in the research.
28627460	3	125	theme	Chinese	535:541	arg1	medicine					543:550	traditional Chinese medicine	523:550	traditional Chinese medicine	523:550	Accumulating evidence indicated polysaccharides derived from traditional Chinese medicine have potent immune-enhancing properties and relatively nontoxic effects in cancer treatment.
28627460	4	126	theme	immunological	672:684	arg1	regulation					686:695	immunological regulation	672:695	immunological regulation in tumor of Epimedium koreanum Nakai polysaccharides	672:748	However, information about immunological regulation in tumor of Epimedium koreanum Nakai polysaccharides is limited and the reports of purification, characterization of polysaccharides have remained less.
28627460	3	127	theme	immune-enhancing	564:579	arg1	properties					581:590	potent immune-enhancing properties	557:590	potent immune-enhancing properties	557:590	Accumulating evidence indicated polysaccharides derived from traditional Chinese medicine have potent immune-enhancing properties and relatively nontoxic effects in cancer treatment.
28627460	1	128	theme	thousand	242:249	arg1	years					251:255	a thousand years	240:255	a thousand years	240:255	ETHNOPHARMACOLOGICAL RELEVANCE Epimedium koreanum Nakai is documented as tonic herbal in China for over a thousand years and has the potential to enhance the body's immunity according to the theory of traditional Chinese medicine.
28627460	12	129	theme	CD4	2193:2195	arg1	differentiation					2204:2218	CD4 T-cell differentiation	2193:2218	CD4 T-cell differentiation	2193:2218	Strikingly, CD4 T-cell differentiation and increased INF-γ production stimulated by EPS-activated macrophages were observed in the research.
28627460	6	130	theme	anti-cancer	1355:1365	arg1	cytokines					1367:1375	anti-cancer cytokines	1355:1375	anti-cancer cytokines	1355:1375	MATERIALS AND METHODS We firstly purified Epimedium koreanum polysaccharide (EPS) from crude extracts and evaluated EPS in vitro using immunological experiments including maturation and Ag presentation function of DCs, CD4 T-cell differentiation and secretion of anti-cancer cytokines.
27117744	0	0	theme	composites	99:108	arg1	characterization					11:26	characterization	11:26	characterization	11:26	Synthesis, characterization, and in vitro cytocompatibility of Ga-bioactive glass/polymer hydrogel composites.
27117744	0	0	theme	composites	99:108	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis, characterization, and in vitro cytocompatibility of Ga-bioactive glass/polymer hydrogel composites.
27117744	0	0	theme	composites	99:108	arg1	cytocompatibility					42:58	in vitro cytocompatibility	33:58	in vitro cytocompatibility	33:58	Synthesis, characterization, and in vitro cytocompatibility of Ga-bioactive glass/polymer hydrogel composites.
27117744	1	1	theme	resulting	310:318	arg1	composites					320:329	the resulting composites	306:329	the resulting composites	306:329	A bioactive glass series (0.42SiO2-0.10Na2O-0.08CaO-(0.40-x)ZnO-(x)Ga2O3) was incorporated in carboxymethyl cellulose-dextran hydrogels at three different loadings (0.05, 0.10, and 0.25 m2), and the resulting composites were characterized using scanning electron microscopy, physical swelling characteristics, and inductively coupled plasma optical emission spectroscopy.
27117744	4	2	theme	incubation	1177:1186	arg1	days					1169:1172	30 days	1166:1172	30 days of incubation in phosphate-buffered saline	1166:1215	Several trends were observed in the inductively coupled plasma optical emission spectroscopy data, with the most important being the release of Ga3+ from both Ga-containing glasses at all three loadings, with a maximum of 4.7 mg/L released after 30 days of incubation in phosphate-buffered saline.
27117744	1	3	theme	ZnO-	171:174	arg1	Ga2O3					178:182	0.42SiO2-0.10Na2O-0.08CaO-(0.40-x)ZnO-(x)Ga2O3	137:182	0.42SiO2-0.10Na2O-0.08CaO-(0.40-x)ZnO-(x)Ga2O3	137:182	A bioactive glass series (0.42SiO2-0.10Na2O-0.08CaO-(0.40-x)ZnO-(x)Ga2O3) was incorporated in carboxymethyl cellulose-dextran hydrogels at three different loadings (0.05, 0.10, and 0.25 m2), and the resulting composites were characterized using scanning electron microscopy, physical swelling characteristics, and inductively coupled plasma optical emission spectroscopy.
27117744	1	3	theme	ZnO-	171:174	arg1	series					129:134	A bioactive glass series	111:134	A bioactive glass series (0.42SiO2-0.10Na2O-0.08CaO-(0.40-x)ZnO-(x)Ga2O3)	111:183	A bioactive glass series (0.42SiO2-0.10Na2O-0.08CaO-(0.40-x)ZnO-(x)Ga2O3) was incorporated in carboxymethyl cellulose-dextran hydrogels at three different loadings (0.05, 0.10, and 0.25 m2), and the resulting composites were characterized using scanning electron microscopy, physical swelling characteristics, and inductively coupled plasma optical emission spectroscopy.
27117744	1	4	theme	different	256:264	arg1	loadings					266:273	three different loadings	250:273	three different loadings (0.05, 0.10, and 0.25 m2)	250:299	A bioactive glass series (0.42SiO2-0.10Na2O-0.08CaO-(0.40-x)ZnO-(x)Ga2O3) was incorporated in carboxymethyl cellulose-dextran hydrogels at three different loadings (0.05, 0.10, and 0.25 m2), and the resulting composites were characterized using scanning electron microscopy, physical swelling characteristics, and inductively coupled plasma optical emission spectroscopy.
27117744	0	5	theme	hydrogel	90:97	arg1	composites					99:108	Ga-bioactive glass/polymer hydrogel composites	63:108	Ga-bioactive glass/polymer hydrogel composites	63:108	Synthesis, characterization, and in vitro cytocompatibility of Ga-bioactive glass/polymer hydrogel composites.
27117744	1	6	theme	coupled	437:443	arg1	spectroscopy					469:480	inductively coupled plasma optical emission spectroscopy	425:480	inductively coupled plasma optical emission spectroscopy	425:480	A bioactive glass series (0.42SiO2-0.10Na2O-0.08CaO-(0.40-x)ZnO-(x)Ga2O3) was incorporated in carboxymethyl cellulose-dextran hydrogels at three different loadings (0.05, 0.10, and 0.25 m2), and the resulting composites were characterized using scanning electron microscopy, physical swelling characteristics, and inductively coupled plasma optical emission spectroscopy.
27117744	4	7	theme	optical	983:989	arg1	spectroscopy					1000:1011	the inductively coupled plasma optical emission spectroscopy	952:1011	the inductively coupled plasma optical emission spectroscopy data	952:1016	Several trends were observed in the inductively coupled plasma optical emission spectroscopy data, with the most important being the release of Ga3+ from both Ga-containing glasses at all three loadings, with a maximum of 4.7 mg/L released after 30 days of incubation in phosphate-buffered saline.
27117744	1	8	theme	plasma	445:450	arg1	spectroscopy					469:480	inductively coupled plasma optical emission spectroscopy	425:480	inductively coupled plasma optical emission spectroscopy	425:480	A bioactive glass series (0.42SiO2-0.10Na2O-0.08CaO-(0.40-x)ZnO-(x)Ga2O3) was incorporated in carboxymethyl cellulose-dextran hydrogels at three different loadings (0.05, 0.10, and 0.25 m2), and the resulting composites were characterized using scanning electron microscopy, physical swelling characteristics, and inductively coupled plasma optical emission spectroscopy.
27117744	4	9	theme	plasma	976:981	arg1	spectroscopy					1000:1011	the inductively coupled plasma optical emission spectroscopy	952:1011	the inductively coupled plasma optical emission spectroscopy data	952:1016	Several trends were observed in the inductively coupled plasma optical emission spectroscopy data, with the most important being the release of Ga3+ from both Ga-containing glasses at all three loadings, with a maximum of 4.7 mg/L released after 30 days of incubation in phosphate-buffered saline.
27117744	1	10	theme	optical	452:458	arg1	spectroscopy					469:480	inductively coupled plasma optical emission spectroscopy	425:480	inductively coupled plasma optical emission spectroscopy	425:480	A bioactive glass series (0.42SiO2-0.10Na2O-0.08CaO-(0.40-x)ZnO-(x)Ga2O3) was incorporated in carboxymethyl cellulose-dextran hydrogels at three different loadings (0.05, 0.10, and 0.25 m2), and the resulting composites were characterized using scanning electron microscopy, physical swelling characteristics, and inductively coupled plasma optical emission spectroscopy.
27117744	4	11	theme	spectroscopy	1000:1011	arg1	data					1013:1016	the inductively coupled plasma optical emission spectroscopy data	952:1016	the inductively coupled plasma optical emission spectroscopy data	952:1016	Several trends were observed in the inductively coupled plasma optical emission spectroscopy data, with the most important being the release of Ga3+ from both Ga-containing glasses at all three loadings, with a maximum of 4.7 mg/L released after 30 days of incubation in phosphate-buffered saline.
27117744	1	12	theme	emission	460:467	arg1	spectroscopy					469:480	inductively coupled plasma optical emission spectroscopy	425:480	inductively coupled plasma optical emission spectroscopy	425:480	A bioactive glass series (0.42SiO2-0.10Na2O-0.08CaO-(0.40-x)ZnO-(x)Ga2O3) was incorporated in carboxymethyl cellulose-dextran hydrogels at three different loadings (0.05, 0.10, and 0.25 m2), and the resulting composites were characterized using scanning electron microscopy, physical swelling characteristics, and inductively coupled plasma optical emission spectroscopy.
27117744	4	13	theme	4.7 mg/L	1142:1149	arg1	maximum					1131:1137	a maximum	1129:1137	a maximum of 4.7 mg/L released after 30 days of incubation in phosphate-buffered saline	1129:1215	Several trends were observed in the inductively coupled plasma optical emission spectroscopy data, with the most important being the release of Ga3+ from both Ga-containing glasses at all three loadings, with a maximum of 4.7 mg/L released after 30 days of incubation in phosphate-buffered saline.
27117744	1	14	theme	x	176:176	arg1	Ga2O3					178:182	0.42SiO2-0.10Na2O-0.08CaO-(0.40-x)ZnO-(x)Ga2O3	137:182	0.42SiO2-0.10Na2O-0.08CaO-(0.40-x)ZnO-(x)Ga2O3	137:182	A bioactive glass series (0.42SiO2-0.10Na2O-0.08CaO-(0.40-x)ZnO-(x)Ga2O3) was incorporated in carboxymethyl cellulose-dextran hydrogels at three different loadings (0.05, 0.10, and 0.25 m2), and the resulting composites were characterized using scanning electron microscopy, physical swelling characteristics, and inductively coupled plasma optical emission spectroscopy.
27117744	1	14	theme	x	176:176	arg1	series					129:134	A bioactive glass series	111:134	A bioactive glass series (0.42SiO2-0.10Na2O-0.08CaO-(0.40-x)ZnO-(x)Ga2O3)	111:183	A bioactive glass series (0.42SiO2-0.10Na2O-0.08CaO-(0.40-x)ZnO-(x)Ga2O3) was incorporated in carboxymethyl cellulose-dextran hydrogels at three different loadings (0.05, 0.10, and 0.25 m2), and the resulting composites were characterized using scanning electron microscopy, physical swelling characteristics, and inductively coupled plasma optical emission spectroscopy.
27117744	2	15	theme	composite	533:541	arg1	extracts					543:550	composite extracts	533:550	composite extracts	533:550	In vitro cytocompatibility was also evaluated for composite extracts in contact with L-929 mouse fibroblasts and MC3T3-E1 human osteoblasts.
27117744	1	16	theme	scanning	356:363	arg1	microscopy					374:383	scanning electron microscopy	356:383	scanning electron microscopy	356:383	A bioactive glass series (0.42SiO2-0.10Na2O-0.08CaO-(0.40-x)ZnO-(x)Ga2O3) was incorporated in carboxymethyl cellulose-dextran hydrogels at three different loadings (0.05, 0.10, and 0.25 m2), and the resulting composites were characterized using scanning electron microscopy, physical swelling characteristics, and inductively coupled plasma optical emission spectroscopy.
27117744	3	17	theme	immersion	866:874	arg1	days					858:861	seven days	852:861	seven days of immersion in phosphate-buffered saline	852:903	Scanning electron microscopy confirmed that glass particles were distributed throughout the hydrogels, and swelling studies showed that glass presence can increase the amount of fluid that can be absorbed by the hydrogels after seven days of immersion in phosphate-buffered saline by up to 180%.
27117744	4	18	theme	coupled	968:974	arg1	spectroscopy					1000:1011	the inductively coupled plasma optical emission spectroscopy	952:1011	the inductively coupled plasma optical emission spectroscopy data	952:1016	Several trends were observed in the inductively coupled plasma optical emission spectroscopy data, with the most important being the release of Ga3+ from both Ga-containing glasses at all three loadings, with a maximum of 4.7 mg/L released after 30 days of incubation in phosphate-buffered saline.
27117744	6	19	theme	bioactive	1395:1403	arg1	particles					1411:1419	embed bioactive glass particles	1389:1419	embed bioactive glass particles into carboxymethyl cellulose-dextran hydrogels	1389:1466	These results indicate that it is possible to embed bioactive glass particles into carboxymethyl cellulose-dextran hydrogels, and upon submersion in aqueous media, release ions from the glass particles that may elicit therapeutic effects.
27117744	1	20	theme	electron	365:372	arg1	microscopy					374:383	scanning electron microscopy	356:383	scanning electron microscopy	356:383	A bioactive glass series (0.42SiO2-0.10Na2O-0.08CaO-(0.40-x)ZnO-(x)Ga2O3) was incorporated in carboxymethyl cellulose-dextran hydrogels at three different loadings (0.05, 0.10, and 0.25 m2), and the resulting composites were characterized using scanning electron microscopy, physical swelling characteristics, and inductively coupled plasma optical emission spectroscopy.
27117744	3	21	dep	180	914:916	arg1	to					911:912	to	911:912	to	911:912	Scanning electron microscopy confirmed that glass particles were distributed throughout the hydrogels, and swelling studies showed that glass presence can increase the amount of fluid that can be absorbed by the hydrogels after seven days of immersion in phosphate-buffered saline by up to 180%.
27117744	4	22	theme	Several	920:926	arg1	trends					928:933	Several trends	920:933	Several trends	920:933	Several trends were observed in the inductively coupled plasma optical emission spectroscopy data, with the most important being the release of Ga3+ from both Ga-containing glasses at all three loadings, with a maximum of 4.7 mg/L released after 30 days of incubation in phosphate-buffered saline.
27117744	3	23	from	immersion	866:874	arg1	saline					898:903	phosphate-buffered saline	879:903	phosphate-buffered saline	879:903	Scanning electron microscopy confirmed that glass particles were distributed throughout the hydrogels, and swelling studies showed that glass presence can increase the amount of fluid that can be absorbed by the hydrogels after seven days of immersion in phosphate-buffered saline by up to 180%.
27117744	3	24	theme	fluid	802:806	arg1	fluid					802:806	fluid	802:806	fluid	802:806	Scanning electron microscopy confirmed that glass particles were distributed throughout the hydrogels, and swelling studies showed that glass presence can increase the amount of fluid that can be absorbed by the hydrogels after seven days of immersion in phosphate-buffered saline by up to 180%.
27117744	3	24	theme	fluid	802:806	arg1	amount					792:797	the amount	788:797	the amount of fluid that can be absorbed by the hydrogels after seven days of immersion in phosphate-buffered saline by up to 180%	788:917	Scanning electron microscopy confirmed that glass particles were distributed throughout the hydrogels, and swelling studies showed that glass presence can increase the amount of fluid that can be absorbed by the hydrogels after seven days of immersion in phosphate-buffered saline by up to 180%.
27117744	2	25	with	contact	555:561	arg1	fibroblasts					580:590	L-929 mouse fibroblasts	568:590	L-929 mouse fibroblasts	568:590	In vitro cytocompatibility was also evaluated for composite extracts in contact with L-929 mouse fibroblasts and MC3T3-E1 human osteoblasts.
27117744	2	25	with	contact	555:561	arg1	osteoblasts					611:621	MC3T3-E1 human osteoblasts	596:621	MC3T3-E1 human osteoblasts	596:621	In vitro cytocompatibility was also evaluated for composite extracts in contact with L-929 mouse fibroblasts and MC3T3-E1 human osteoblasts.
27117744	3	26	from	saline	898:903	arg1	days					858:861	seven days	852:861	seven days of immersion in phosphate-buffered saline	852:903	Scanning electron microscopy confirmed that glass particles were distributed throughout the hydrogels, and swelling studies showed that glass presence can increase the amount of fluid that can be absorbed by the hydrogels after seven days of immersion in phosphate-buffered saline by up to 180%.
27117744	6	27	theme	aqueous	1492:1498	arg1	media					1500:1504	aqueous media	1492:1504	aqueous media	1492:1504	These results indicate that it is possible to embed bioactive glass particles into carboxymethyl cellulose-dextran hydrogels, and upon submersion in aqueous media, release ions from the glass particles that may elicit therapeutic effects.
27117744	3	28	from	days	858:861	arg1	saline					898:903	phosphate-buffered saline	879:903	phosphate-buffered saline	879:903	Scanning electron microscopy confirmed that glass particles were distributed throughout the hydrogels, and swelling studies showed that glass presence can increase the amount of fluid that can be absorbed by the hydrogels after seven days of immersion in phosphate-buffered saline by up to 180%.
27117744	6	29	theme	embed	1389:1393	arg1	particles					1411:1419	embed bioactive glass particles	1389:1419	embed bioactive glass particles into carboxymethyl cellulose-dextran hydrogels	1389:1466	These results indicate that it is possible to embed bioactive glass particles into carboxymethyl cellulose-dextran hydrogels, and upon submersion in aqueous media, release ions from the glass particles that may elicit therapeutic effects.
27117744	1	30	theme	bioactive	113:121	arg1	Ga2O3					178:182	0.42SiO2-0.10Na2O-0.08CaO-(0.40-x)ZnO-(x)Ga2O3	137:182	0.42SiO2-0.10Na2O-0.08CaO-(0.40-x)ZnO-(x)Ga2O3	137:182	A bioactive glass series (0.42SiO2-0.10Na2O-0.08CaO-(0.40-x)ZnO-(x)Ga2O3) was incorporated in carboxymethyl cellulose-dextran hydrogels at three different loadings (0.05, 0.10, and 0.25 m2), and the resulting composites were characterized using scanning electron microscopy, physical swelling characteristics, and inductively coupled plasma optical emission spectroscopy.
27117744	1	30	theme	bioactive	113:121	arg1	series					129:134	A bioactive glass series	111:134	A bioactive glass series (0.42SiO2-0.10Na2O-0.08CaO-(0.40-x)ZnO-(x)Ga2O3)	111:183	A bioactive glass series (0.42SiO2-0.10Na2O-0.08CaO-(0.40-x)ZnO-(x)Ga2O3) was incorporated in carboxymethyl cellulose-dextran hydrogels at three different loadings (0.05, 0.10, and 0.25 m2), and the resulting composites were characterized using scanning electron microscopy, physical swelling characteristics, and inductively coupled plasma optical emission spectroscopy.
27117744	6	31	from	particles	1535:1543	arg1	ions					1515:1518	ions	1515:1518	ions from the glass particles that may elicit therapeutic effects	1515:1579	These results indicate that it is possible to embed bioactive glass particles into carboxymethyl cellulose-dextran hydrogels, and upon submersion in aqueous media, release ions from the glass particles that may elicit therapeutic effects.
27117744	4	32	theme	Ga3+	1064:1067	arg1	important					1033:1041	important	1033:1041	important	1033:1041	Several trends were observed in the inductively coupled plasma optical emission spectroscopy data, with the most important being the release of Ga3+ from both Ga-containing glasses at all three loadings, with a maximum of 4.7 mg/L released after 30 days of incubation in phosphate-buffered saline.
27117744	4	32	theme	Ga3+	1064:1067	arg1	release					1053:1059	the release	1049:1059	the release of Ga3+ from both Ga-containing glasses	1049:1099	Several trends were observed in the inductively coupled plasma optical emission spectroscopy data, with the most important being the release of Ga3+ from both Ga-containing glasses at all three loadings, with a maximum of 4.7 mg/L released after 30 days of incubation in phosphate-buffered saline.
27117744	2	33	theme	mouse	574:578	arg1	fibroblasts					580:590	L-929 mouse fibroblasts	568:590	L-929 mouse fibroblasts	568:590	In vitro cytocompatibility was also evaluated for composite extracts in contact with L-929 mouse fibroblasts and MC3T3-E1 human osteoblasts.
27117744	1	34	theme	glass	123:127	arg1	Ga2O3					178:182	0.42SiO2-0.10Na2O-0.08CaO-(0.40-x)ZnO-(x)Ga2O3	137:182	0.42SiO2-0.10Na2O-0.08CaO-(0.40-x)ZnO-(x)Ga2O3	137:182	A bioactive glass series (0.42SiO2-0.10Na2O-0.08CaO-(0.40-x)ZnO-(x)Ga2O3) was incorporated in carboxymethyl cellulose-dextran hydrogels at three different loadings (0.05, 0.10, and 0.25 m2), and the resulting composites were characterized using scanning electron microscopy, physical swelling characteristics, and inductively coupled plasma optical emission spectroscopy.
27117744	1	34	theme	glass	123:127	arg1	series					129:134	A bioactive glass series	111:134	A bioactive glass series (0.42SiO2-0.10Na2O-0.08CaO-(0.40-x)ZnO-(x)Ga2O3)	111:183	A bioactive glass series (0.42SiO2-0.10Na2O-0.08CaO-(0.40-x)ZnO-(x)Ga2O3) was incorporated in carboxymethyl cellulose-dextran hydrogels at three different loadings (0.05, 0.10, and 0.25 m2), and the resulting composites were characterized using scanning electron microscopy, physical swelling characteristics, and inductively coupled plasma optical emission spectroscopy.
27117744	6	35	theme	therapeutic	1561:1571	arg1	effects					1573:1579	therapeutic effects	1561:1579	therapeutic effects	1561:1579	These results indicate that it is possible to embed bioactive glass particles into carboxymethyl cellulose-dextran hydrogels, and upon submersion in aqueous media, release ions from the glass particles that may elicit therapeutic effects.
27117744	0	36	theme	in vitro	33:40	arg1	cytocompatibility					42:58	in vitro cytocompatibility	33:58	in vitro cytocompatibility	33:58	Synthesis, characterization, and in vitro cytocompatibility of Ga-bioactive glass/polymer hydrogel composites.
27117744	5	37	theme	Cell	1218:1221	arg1	analysis					1233:1240	Cell viability analysis	1218:1240	Cell viability analysis	1218:1240	Cell viability analysis suggested that most composite extracts did not decrease neither fibroblast nor osteoblast viability.
27117744	2	38	theme	L-929	568:572	arg1	fibroblasts					580:590	L-929 mouse fibroblasts	568:590	L-929 mouse fibroblasts	568:590	In vitro cytocompatibility was also evaluated for composite extracts in contact with L-929 mouse fibroblasts and MC3T3-E1 human osteoblasts.
27117744	5	39	theme	most	1257:1260	arg1	extracts					1272:1279	most composite extracts	1257:1279	most composite extracts	1257:1279	Cell viability analysis suggested that most composite extracts did not decrease neither fibroblast nor osteoblast viability.
27117744	3	40	theme	electron	633:640	arg1	microscopy					642:651	Scanning electron microscopy	624:651	Scanning electron microscopy	624:651	Scanning electron microscopy confirmed that glass particles were distributed throughout the hydrogels, and swelling studies showed that glass presence can increase the amount of fluid that can be absorbed by the hydrogels after seven days of immersion in phosphate-buffered saline by up to 180%.
27117744	4	41	located	observed	940:947	arg2	trends					928:933	Several trends	920:933	Several trends	920:933	Several trends were observed in the inductively coupled plasma optical emission spectroscopy data, with the most important being the release of Ga3+ from both Ga-containing glasses at all three loadings, with a maximum of 4.7 mg/L released after 30 days of incubation in phosphate-buffered saline.
27117744	4	41	located	observed	940:947	arg1	data					1013:1016	the inductively coupled plasma optical emission spectroscopy data	952:1016	the inductively coupled plasma optical emission spectroscopy data	952:1016	Several trends were observed in the inductively coupled plasma optical emission spectroscopy data, with the most important being the release of Ga3+ from both Ga-containing glasses at all three loadings, with a maximum of 4.7 mg/L released after 30 days of incubation in phosphate-buffered saline.
27117744	2	42	theme	In vitro	483:490	arg1	cytocompatibility					492:508	In vitro cytocompatibility	483:508	In vitro cytocompatibility	483:508	In vitro cytocompatibility was also evaluated for composite extracts in contact with L-929 mouse fibroblasts and MC3T3-E1 human osteoblasts.
27117744	6	43	theme	cellulose-dextran	1440:1456	arg1	hydrogels					1458:1466	carboxymethyl cellulose-dextran hydrogels	1426:1466	carboxymethyl cellulose-dextran hydrogels	1426:1466	These results indicate that it is possible to embed bioactive glass particles into carboxymethyl cellulose-dextran hydrogels, and upon submersion in aqueous media, release ions from the glass particles that may elicit therapeutic effects.
27117744	1	44	from	loadings	266:273	arg1	hydrogels					237:245	carboxymethyl cellulose-dextran hydrogels	205:245	carboxymethyl cellulose-dextran hydrogels at three different loadings (0.05, 0.10, and 0.25 m2)	205:299	A bioactive glass series (0.42SiO2-0.10Na2O-0.08CaO-(0.40-x)ZnO-(x)Ga2O3) was incorporated in carboxymethyl cellulose-dextran hydrogels at three different loadings (0.05, 0.10, and 0.25 m2), and the resulting composites were characterized using scanning electron microscopy, physical swelling characteristics, and inductively coupled plasma optical emission spectroscopy.
27117744	1	45	theme	physical	386:393	arg1	characteristics					404:418	physical swelling characteristics	386:418	physical swelling characteristics	386:418	A bioactive glass series (0.42SiO2-0.10Na2O-0.08CaO-(0.40-x)ZnO-(x)Ga2O3) was incorporated in carboxymethyl cellulose-dextran hydrogels at three different loadings (0.05, 0.10, and 0.25 m2), and the resulting composites were characterized using scanning electron microscopy, physical swelling characteristics, and inductively coupled plasma optical emission spectroscopy.
27117744	1	46	theme	carboxymethyl	205:217	arg1	hydrogels					237:245	carboxymethyl cellulose-dextran hydrogels	205:245	carboxymethyl cellulose-dextran hydrogels at three different loadings (0.05, 0.10, and 0.25 m2)	205:299	A bioactive glass series (0.42SiO2-0.10Na2O-0.08CaO-(0.40-x)ZnO-(x)Ga2O3) was incorporated in carboxymethyl cellulose-dextran hydrogels at three different loadings (0.05, 0.10, and 0.25 m2), and the resulting composites were characterized using scanning electron microscopy, physical swelling characteristics, and inductively coupled plasma optical emission spectroscopy.
27117744	1	47	theme	swelling	395:402	arg1	characteristics					404:418	physical swelling characteristics	386:418	physical swelling characteristics	386:418	A bioactive glass series (0.42SiO2-0.10Na2O-0.08CaO-(0.40-x)ZnO-(x)Ga2O3) was incorporated in carboxymethyl cellulose-dextran hydrogels at three different loadings (0.05, 0.10, and 0.25 m2), and the resulting composites were characterized using scanning electron microscopy, physical swelling characteristics, and inductively coupled plasma optical emission spectroscopy.
27117744	4	48	from	saline	1210:1215	arg1	days					1169:1172	30 days	1166:1172	30 days of incubation in phosphate-buffered saline	1166:1215	Several trends were observed in the inductively coupled plasma optical emission spectroscopy data, with the most important being the release of Ga3+ from both Ga-containing glasses at all three loadings, with a maximum of 4.7 mg/L released after 30 days of incubation in phosphate-buffered saline.
27117744	4	49	theme	phosphate-buffered	1191:1208	arg1	saline					1210:1215	phosphate-buffered saline	1191:1215	phosphate-buffered saline	1191:1215	Several trends were observed in the inductively coupled plasma optical emission spectroscopy data, with the most important being the release of Ga3+ from both Ga-containing glasses at all three loadings, with a maximum of 4.7 mg/L released after 30 days of incubation in phosphate-buffered saline.
27117744	2	50	theme	human	605:609	arg1	osteoblasts					611:621	MC3T3-E1 human osteoblasts	596:621	MC3T3-E1 human osteoblasts	596:621	In vitro cytocompatibility was also evaluated for composite extracts in contact with L-929 mouse fibroblasts and MC3T3-E1 human osteoblasts.
27117744	3	51	theme	Scanning	624:631	arg1	microscopy					642:651	Scanning electron microscopy	624:651	Scanning electron microscopy	624:651	Scanning electron microscopy confirmed that glass particles were distributed throughout the hydrogels, and swelling studies showed that glass presence can increase the amount of fluid that can be absorbed by the hydrogels after seven days of immersion in phosphate-buffered saline by up to 180%.
27117744	4	52	from	loadings	1114:1121	arg1	important					1033:1041	important	1033:1041	important	1033:1041	Several trends were observed in the inductively coupled plasma optical emission spectroscopy data, with the most important being the release of Ga3+ from both Ga-containing glasses at all three loadings, with a maximum of 4.7 mg/L released after 30 days of incubation in phosphate-buffered saline.
27117744	4	52	from	loadings	1114:1121	arg1	release					1053:1059	the release	1049:1059	the release of Ga3+ from both Ga-containing glasses	1049:1099	Several trends were observed in the inductively coupled plasma optical emission spectroscopy data, with the most important being the release of Ga3+ from both Ga-containing glasses at all three loadings, with a maximum of 4.7 mg/L released after 30 days of incubation in phosphate-buffered saline.
27117744	6	53	theme	carboxymethyl	1426:1438	arg1	hydrogels					1458:1466	carboxymethyl cellulose-dextran hydrogels	1426:1466	carboxymethyl cellulose-dextran hydrogels	1426:1466	These results indicate that it is possible to embed bioactive glass particles into carboxymethyl cellulose-dextran hydrogels, and upon submersion in aqueous media, release ions from the glass particles that may elicit therapeutic effects.
27117744	2	54	theme	MC3T3-E1	596:603	arg1	osteoblasts					611:621	MC3T3-E1 human osteoblasts	596:621	MC3T3-E1 human osteoblasts	596:621	In vitro cytocompatibility was also evaluated for composite extracts in contact with L-929 mouse fibroblasts and MC3T3-E1 human osteoblasts.
27117744	3	55	theme	swelling	731:738	arg1	studies					740:746	swelling studies	731:746	swelling studies	731:746	Scanning electron microscopy confirmed that glass particles were distributed throughout the hydrogels, and swelling studies showed that glass presence can increase the amount of fluid that can be absorbed by the hydrogels after seven days of immersion in phosphate-buffered saline by up to 180%.
27117744	3	56	theme	glass	668:672	arg1	particles					674:682	glass particles	668:682	glass particles	668:682	Scanning electron microscopy confirmed that glass particles were distributed throughout the hydrogels, and swelling studies showed that glass presence can increase the amount of fluid that can be absorbed by the hydrogels after seven days of immersion in phosphate-buffered saline by up to 180%.
27117744	1	57	theme	0.42SiO2-0.10Na2O-0.08CaO-	137:162	arg1	Ga2O3					178:182	0.42SiO2-0.10Na2O-0.08CaO-(0.40-x)ZnO-(x)Ga2O3	137:182	0.42SiO2-0.10Na2O-0.08CaO-(0.40-x)ZnO-(x)Ga2O3	137:182	A bioactive glass series (0.42SiO2-0.10Na2O-0.08CaO-(0.40-x)ZnO-(x)Ga2O3) was incorporated in carboxymethyl cellulose-dextran hydrogels at three different loadings (0.05, 0.10, and 0.25 m2), and the resulting composites were characterized using scanning electron microscopy, physical swelling characteristics, and inductively coupled plasma optical emission spectroscopy.
27117744	1	57	theme	0.42SiO2-0.10Na2O-0.08CaO-	137:162	arg1	series					129:134	A bioactive glass series	111:134	A bioactive glass series (0.42SiO2-0.10Na2O-0.08CaO-(0.40-x)ZnO-(x)Ga2O3)	111:183	A bioactive glass series (0.42SiO2-0.10Na2O-0.08CaO-(0.40-x)ZnO-(x)Ga2O3) was incorporated in carboxymethyl cellulose-dextran hydrogels at three different loadings (0.05, 0.10, and 0.25 m2), and the resulting composites were characterized using scanning electron microscopy, physical swelling characteristics, and inductively coupled plasma optical emission spectroscopy.
27117744	5	58	theme	osteoblast	1321:1330	arg1	viability					1332:1340	neither fibroblast nor osteoblast viability	1298:1340	viability	1332:1340	Cell viability analysis suggested that most composite extracts did not decrease neither fibroblast nor osteoblast viability.
27117744	1	59	theme	cellulose-dextran	219:235	arg1	hydrogels					237:245	carboxymethyl cellulose-dextran hydrogels	205:245	carboxymethyl cellulose-dextran hydrogels at three different loadings (0.05, 0.10, and 0.25 m2)	205:299	A bioactive glass series (0.42SiO2-0.10Na2O-0.08CaO-(0.40-x)ZnO-(x)Ga2O3) was incorporated in carboxymethyl cellulose-dextran hydrogels at three different loadings (0.05, 0.10, and 0.25 m2), and the resulting composites were characterized using scanning electron microscopy, physical swelling characteristics, and inductively coupled plasma optical emission spectroscopy.
27117744	6	60	from	submersion	1478:1487	arg1	media					1500:1504	aqueous media	1492:1504	aqueous media	1492:1504	These results indicate that it is possible to embed bioactive glass particles into carboxymethyl cellulose-dextran hydrogels, and upon submersion in aqueous media, release ions from the glass particles that may elicit therapeutic effects.
27117744	4	61	from	incubation	1177:1186	arg1	saline					1210:1215	phosphate-buffered saline	1191:1215	phosphate-buffered saline	1191:1215	Several trends were observed in the inductively coupled plasma optical emission spectroscopy data, with the most important being the release of Ga3+ from both Ga-containing glasses at all three loadings, with a maximum of 4.7 mg/L released after 30 days of incubation in phosphate-buffered saline.
27117744	5	62	theme	viability	1223:1231	arg1	analysis					1233:1240	Cell viability analysis	1218:1240	Cell viability analysis	1218:1240	Cell viability analysis suggested that most composite extracts did not decrease neither fibroblast nor osteoblast viability.
27117744	4	63	from	glasses	1093:1099	arg1	important					1033:1041	important	1033:1041	important	1033:1041	Several trends were observed in the inductively coupled plasma optical emission spectroscopy data, with the most important being the release of Ga3+ from both Ga-containing glasses at all three loadings, with a maximum of 4.7 mg/L released after 30 days of incubation in phosphate-buffered saline.
27117744	4	63	from	glasses	1093:1099	arg1	release					1053:1059	the release	1049:1059	the release of Ga3+ from both Ga-containing glasses	1049:1099	Several trends were observed in the inductively coupled plasma optical emission spectroscopy data, with the most important being the release of Ga3+ from both Ga-containing glasses at all three loadings, with a maximum of 4.7 mg/L released after 30 days of incubation in phosphate-buffered saline.
27117744	3	64	theme	phosphate-buffered	879:896	arg1	saline					898:903	phosphate-buffered saline	879:903	phosphate-buffered saline	879:903	Scanning electron microscopy confirmed that glass particles were distributed throughout the hydrogels, and swelling studies showed that glass presence can increase the amount of fluid that can be absorbed by the hydrogels after seven days of immersion in phosphate-buffered saline by up to 180%.
27117744	5	65	theme	composite	1262:1270	arg1	extracts					1272:1279	most composite extracts	1257:1279	most composite extracts	1257:1279	Cell viability analysis suggested that most composite extracts did not decrease neither fibroblast nor osteoblast viability.
27117744	0	66	theme	glass/polymer	76:88	arg1	composites					99:108	Ga-bioactive glass/polymer hydrogel composites	63:108	Ga-bioactive glass/polymer hydrogel composites	63:108	Synthesis, characterization, and in vitro cytocompatibility of Ga-bioactive glass/polymer hydrogel composites.
27117744	1	67	dep	loadings	266:273	arg1	0.10					282:285	0.10	282:285	0.10	282:285	A bioactive glass series (0.42SiO2-0.10Na2O-0.08CaO-(0.40-x)ZnO-(x)Ga2O3) was incorporated in carboxymethyl cellulose-dextran hydrogels at three different loadings (0.05, 0.10, and 0.25 m2), and the resulting composites were characterized using scanning electron microscopy, physical swelling characteristics, and inductively coupled plasma optical emission spectroscopy.
27117744	1	67	dep	loadings	266:273	arg1	0.25 m2					292:298	0.25 m2	292:298	0.25 m2	292:298	A bioactive glass series (0.42SiO2-0.10Na2O-0.08CaO-(0.40-x)ZnO-(x)Ga2O3) was incorporated in carboxymethyl cellulose-dextran hydrogels at three different loadings (0.05, 0.10, and 0.25 m2), and the resulting composites were characterized using scanning electron microscopy, physical swelling characteristics, and inductively coupled plasma optical emission spectroscopy.
27117744	1	67	dep	loadings	266:273	arg1	0.05					276:279	0.05	276:279	0.05	276:279	A bioactive glass series (0.42SiO2-0.10Na2O-0.08CaO-(0.40-x)ZnO-(x)Ga2O3) was incorporated in carboxymethyl cellulose-dextran hydrogels at three different loadings (0.05, 0.10, and 0.25 m2), and the resulting composites were characterized using scanning electron microscopy, physical swelling characteristics, and inductively coupled plasma optical emission spectroscopy.
27117744	1	68	theme	0.40-x	164:169	arg1	Ga2O3					178:182	0.42SiO2-0.10Na2O-0.08CaO-(0.40-x)ZnO-(x)Ga2O3	137:182	0.42SiO2-0.10Na2O-0.08CaO-(0.40-x)ZnO-(x)Ga2O3	137:182	A bioactive glass series (0.42SiO2-0.10Na2O-0.08CaO-(0.40-x)ZnO-(x)Ga2O3) was incorporated in carboxymethyl cellulose-dextran hydrogels at three different loadings (0.05, 0.10, and 0.25 m2), and the resulting composites were characterized using scanning electron microscopy, physical swelling characteristics, and inductively coupled plasma optical emission spectroscopy.
27117744	1	68	theme	0.40-x	164:169	arg1	series					129:134	A bioactive glass series	111:134	A bioactive glass series (0.42SiO2-0.10Na2O-0.08CaO-(0.40-x)ZnO-(x)Ga2O3)	111:183	A bioactive glass series (0.42SiO2-0.10Na2O-0.08CaO-(0.40-x)ZnO-(x)Ga2O3) was incorporated in carboxymethyl cellulose-dextran hydrogels at three different loadings (0.05, 0.10, and 0.25 m2), and the resulting composites were characterized using scanning electron microscopy, physical swelling characteristics, and inductively coupled plasma optical emission spectroscopy.
27117744	6	69	theme	glass	1529:1533	arg1	particles					1535:1543	the glass particles	1525:1543	the glass particles	1525:1543	These results indicate that it is possible to embed bioactive glass particles into carboxymethyl cellulose-dextran hydrogels, and upon submersion in aqueous media, release ions from the glass particles that may elicit therapeutic effects.
27117744	0	70	theme	Ga-bioactive	63:74	arg1	composites					99:108	Ga-bioactive glass/polymer hydrogel composites	63:108	Ga-bioactive glass/polymer hydrogel composites	63:108	Synthesis, characterization, and in vitro cytocompatibility of Ga-bioactive glass/polymer hydrogel composites.
27117744	4	71	from	days	1169:1172	arg1	saline					1210:1215	phosphate-buffered saline	1191:1215	phosphate-buffered saline	1191:1215	Several trends were observed in the inductively coupled plasma optical emission spectroscopy data, with the most important being the release of Ga3+ from both Ga-containing glasses at all three loadings, with a maximum of 4.7 mg/L released after 30 days of incubation in phosphate-buffered saline.
27117744	3	72	theme	glass	760:764	arg1	presence					766:773	glass presence	760:773	glass presence	760:773	Scanning electron microscopy confirmed that glass particles were distributed throughout the hydrogels, and swelling studies showed that glass presence can increase the amount of fluid that can be absorbed by the hydrogels after seven days of immersion in phosphate-buffered saline by up to 180%.
27117744	4	73	theme	emission	991:998	arg1	spectroscopy					1000:1011	the inductively coupled plasma optical emission spectroscopy	952:1011	the inductively coupled plasma optical emission spectroscopy data	952:1016	Several trends were observed in the inductively coupled plasma optical emission spectroscopy data, with the most important being the release of Ga3+ from both Ga-containing glasses at all three loadings, with a maximum of 4.7 mg/L released after 30 days of incubation in phosphate-buffered saline.
27117744	4	74	theme	Ga-containing	1079:1091	arg1	glasses					1093:1099	both Ga-containing glasses	1074:1099	both Ga-containing glasses	1074:1099	Several trends were observed in the inductively coupled plasma optical emission spectroscopy data, with the most important being the release of Ga3+ from both Ga-containing glasses at all three loadings, with a maximum of 4.7 mg/L released after 30 days of incubation in phosphate-buffered saline.
27117744	6	75	theme	glass	1405:1409	arg1	particles					1411:1419	embed bioactive glass particles	1389:1419	embed bioactive glass particles into carboxymethyl cellulose-dextran hydrogels	1389:1466	These results indicate that it is possible to embed bioactive glass particles into carboxymethyl cellulose-dextran hydrogels, and upon submersion in aqueous media, release ions from the glass particles that may elicit therapeutic effects.
24128572	6	0	theme	monosaccharides	1023:1037	arg1	mixture					1012:1018	PMP-labelled mixture	999:1018	PMP-labelled mixture of monosaccharides	999:1037	PMP-labelled mixture of monosaccharides has been well separated by a reverse-phase HPLC and detected by on-line ESI-MS method under optimised conditions.
24128572	2	1	theme	S.	718:719	arg1	fusiforme					721:729	S. fusiforme	718:729	S. fusiforme	718:729	A facile and sensitive method based on high-performance liquid chromatography method of pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP) coupled with electrospray ionisation mass spectrometry (HPLC/ESI-MS) has been established for the analysis of the monosaccharide composition of polysaccharides in S. fusiforme.
24128572	2	2	theme	polysaccharides	699:713	arg1	composition					684:694	the monosaccharide composition	665:694	the monosaccharide composition of polysaccharides in S. fusiforme	665:729	A facile and sensitive method based on high-performance liquid chromatography method of pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP) coupled with electrospray ionisation mass spectrometry (HPLC/ESI-MS) has been established for the analysis of the monosaccharide composition of polysaccharides in S. fusiforme.
24128572	2	3	from	composition	684:694	arg1	fusiforme					721:729	S. fusiforme	718:729	S. fusiforme	718:729	A facile and sensitive method based on high-performance liquid chromatography method of pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP) coupled with electrospray ionisation mass spectrometry (HPLC/ESI-MS) has been established for the analysis of the monosaccharide composition of polysaccharides in S. fusiforme.
24128572	3	4	with	derivatives	786:796	arg1	ammonia					811:817	aqueous ammonia	803:817	aqueous ammonia	803:817	Monosaccharides have been converted into PMP-labelled derivatives with aqueous ammonia as a catalyst at 70 °C for 30 min.
24128572	2	5	theme	monosaccharide	669:682	arg1	composition					684:694	the monosaccharide composition	665:694	the monosaccharide composition of polysaccharides in S. fusiforme	665:729	A facile and sensitive method based on high-performance liquid chromatography method of pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP) coupled with electrospray ionisation mass spectrometry (HPLC/ESI-MS) has been established for the analysis of the monosaccharide composition of polysaccharides in S. fusiforme.
24128572	3	6	from	°C	839:840	arg1	catalyst					824:831	a catalyst	822:831	a catalyst at 70 °C for 30 min	822:851	Monosaccharides have been converted into PMP-labelled derivatives with aqueous ammonia as a catalyst at 70 °C for 30 min.
24128572	3	6	from	°C	839:840	arg1	Monosaccharides					732:746	Monosaccharides	732:746	Monosaccharides	732:746	Monosaccharides have been converted into PMP-labelled derivatives with aqueous ammonia as a catalyst at 70 °C for 30 min.
24128572	1	7	theme	well-known	222:231	arg1	algae					240:244	the well-known edible algae	218:244	the well-known edible algae	218:244	Sargassum fusiforme (hijiki) is the well-known edible algae, whose polysaccharides have been proved to possess interesting bioactivities like antitumor, antioxidant, antimicrobial and immunomodulatory activities.
24128572	1	7	theme	well-known	222:231	arg1	fusiforme					196:204	Sargassum fusiforme	186:204	Sargassum fusiforme (hijiki)	186:213	Sargassum fusiforme (hijiki) is the well-known edible algae, whose polysaccharides have been proved to possess interesting bioactivities like antitumor, antioxidant, antimicrobial and immunomodulatory activities.
24128572	0	8	theme	ionisation	156:165	arg1	spectrometry					172:183	high performance liquid chromatography/electrospray ionisation mass spectrometry	104:183	high performance liquid chromatography/electrospray ionisation mass spectrometry	104:183	Analysis of the monosaccharide composition of water-soluble polysaccharides from Sargassum fusiforme by high performance liquid chromatography/electrospray ionisation mass spectrometry.
24128572	9	9	theme	PMP-labelled	1584:1595	arg1	derivatives					1597:1607	All PMP-labelled derivatives	1580:1607	All PMP-labelled derivatives	1580:1607	All PMP-labelled derivatives display high chemical stabilities, whose regular MS fragmentation is specific for reducing labelled sugars.
24128572	7	10	theme	elution	1173:1179	arg1	system					1181:1186	elution system	1173:1186	elution system	1173:1186	The mobile phase of elution system was chosen as acetonitrile (solvent A) and 20mM aqueous ammonium acetate (solvent B) (pH 3.0) with Zorbax XDB-C18 column at 30 °C for the separation of the monosaccharide derivatives.
24128572	2	11	theme	composition	684:694	arg1	analysis					653:660	the analysis	649:660	the analysis of the monosaccharide composition of polysaccharides in S. fusiforme	649:729	A facile and sensitive method based on high-performance liquid chromatography method of pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP) coupled with electrospray ionisation mass spectrometry (HPLC/ESI-MS) has been established for the analysis of the monosaccharide composition of polysaccharides in S. fusiforme.
24128572	8	12	theme	monosaccharides	1563:1577	arg1	behaviour					1469:1477	mass spectral behaviour	1455:1477	mass spectral behaviour	1455:1477	Identification of the monosaccharides composition was carried out by analysis with mass spectral behaviour and chromatography characteristics of 1-phenyl-3-methyl-5-pyrazolone (PMP) labelled monosaccharides.
24128572	8	12	theme	monosaccharides	1563:1577	arg1	characteristics					1498:1512	chromatography characteristics	1483:1512	chromatography characteristics	1483:1512	Identification of the monosaccharides composition was carried out by analysis with mass spectral behaviour and chromatography characteristics of 1-phenyl-3-methyl-5-pyrazolone (PMP) labelled monosaccharides.
24128572	10	13	theme	S.	1744:1745	arg1	polysaccharide					1757:1770	the S. fusiforme polysaccharide	1740:1770	the S. fusiforme polysaccharide	1740:1770	The result showed that the S. fusiforme polysaccharide consisted of mannose, glucose, galactose, xylose, fucose and glucuronic acid or galacturonic acid, or both uronic acids.
24128572	9	14	theme	chemical	1622:1629	arg1	stabilities					1631:1641	high chemical stabilities	1617:1641	high chemical stabilities	1617:1641	All PMP-labelled derivatives display high chemical stabilities, whose regular MS fragmentation is specific for reducing labelled sugars.
24128572	8	15	theme	spectral	1460:1467	arg1	behaviour					1469:1477	mass spectral behaviour	1455:1477	mass spectral behaviour	1455:1477	Identification of the monosaccharides composition was carried out by analysis with mass spectral behaviour and chromatography characteristics of 1-phenyl-3-methyl-5-pyrazolone (PMP) labelled monosaccharides.
24128572	6	16	theme	optimised	1131:1139	arg1	conditions					1141:1150	optimised conditions	1131:1150	optimised conditions	1131:1150	PMP-labelled mixture of monosaccharides has been well separated by a reverse-phase HPLC and detected by on-line ESI-MS method under optimised conditions.
24128572	9	17	theme	regular	1650:1656	arg1	fragmentation					1661:1673	fragmentation	1661:1673	fragmentation	1661:1673	All PMP-labelled derivatives display high chemical stabilities, whose regular MS fragmentation is specific for reducing labelled sugars.
24128572	2	18	theme	mass	592:595	arg1	spectrometry					597:608	electrospray ionisation mass spectrometry	568:608	electrospray ionisation mass spectrometry (HPLC/ESI-MS)	568:622	A facile and sensitive method based on high-performance liquid chromatography method of pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP) coupled with electrospray ionisation mass spectrometry (HPLC/ESI-MS) has been established for the analysis of the monosaccharide composition of polysaccharides in S. fusiforme.
24128572	2	18	theme	mass	592:595	arg1	HPLC/ESI-MS					611:621	HPLC/ESI-MS	611:621	HPLC/ESI-MS	611:621	A facile and sensitive method based on high-performance liquid chromatography method of pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP) coupled with electrospray ionisation mass spectrometry (HPLC/ESI-MS) has been established for the analysis of the monosaccharide composition of polysaccharides in S. fusiforme.
24128572	8	19	theme	composition	1410:1420	arg1	Identification					1372:1385	Identification	1372:1385	Identification of the monosaccharides composition	1372:1420	Identification of the monosaccharides composition was carried out by analysis with mass spectral behaviour and chromatography characteristics of 1-phenyl-3-methyl-5-pyrazolone (PMP) labelled monosaccharides.
24128572	7	20	theme	Zorbax	1287:1292	arg1	column					1302:1307	Zorbax XDB-C18 column	1287:1307	Zorbax XDB-C18 column	1287:1307	The mobile phase of elution system was chosen as acetonitrile (solvent A) and 20mM aqueous ammonium acetate (solvent B) (pH 3.0) with Zorbax XDB-C18 column at 30 °C for the separation of the monosaccharide derivatives.
24128572	6	21	theme	on-line	1103:1109	arg1	method					1118:1123	on-line ESI-MS method	1103:1123	on-line ESI-MS method	1103:1123	PMP-labelled mixture of monosaccharides has been well separated by a reverse-phase HPLC and detected by on-line ESI-MS method under optimised conditions.
24128572	0	22	theme	high	104:107	arg1	spectrometry					172:183	high performance liquid chromatography/electrospray ionisation mass spectrometry	104:183	high performance liquid chromatography/electrospray ionisation mass spectrometry	104:183	Analysis of the monosaccharide composition of water-soluble polysaccharides from Sargassum fusiforme by high performance liquid chromatography/electrospray ionisation mass spectrometry.
24128572	4	23	theme	derivatization	889:902	arg1	process					904:910	the pre-column derivatization process	874:910	the pre-column derivatization process	874:910	The optimisation of the pre-column derivatization process was studied.
24128572	2	24	theme	chromatography	462:475	arg1	PMP					550:552	PMP	550:552	PMP	550:552	A facile and sensitive method based on high-performance liquid chromatography method of pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP) coupled with electrospray ionisation mass spectrometry (HPLC/ESI-MS) has been established for the analysis of the monosaccharide composition of polysaccharides in S. fusiforme.
24128572	2	24	theme	chromatography	462:475	arg1	method					477:482	high-performance liquid chromatography method	438:482	high-performance liquid chromatography method of pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP) coupled with electrospray ionisation mass spectrometry (HPLC/ESI-MS)	438:622	A facile and sensitive method based on high-performance liquid chromatography method of pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP) coupled with electrospray ionisation mass spectrometry (HPLC/ESI-MS) has been established for the analysis of the monosaccharide composition of polysaccharides in S. fusiforme.
24128572	0	25	theme	liquid	121:126	arg1	spectrometry					172:183	high performance liquid chromatography/electrospray ionisation mass spectrometry	104:183	high performance liquid chromatography/electrospray ionisation mass spectrometry	104:183	Analysis of the monosaccharide composition of water-soluble polysaccharides from Sargassum fusiforme by high performance liquid chromatography/electrospray ionisation mass spectrometry.
24128572	0	26	from	composition	31:41	arg1	Sargassum					81:89	Sargassum	81:89	Sargassum	81:89	Analysis of the monosaccharide composition of water-soluble polysaccharides from Sargassum fusiforme by high performance liquid chromatography/electrospray ionisation mass spectrometry.
24128572	2	27	theme	high-performance	438:453	arg1	chromatography					462:475	high-performance liquid chromatography	438:475	high-performance liquid chromatography method of pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP) coupled with electrospray ionisation mass spectrometry (HPLC/ESI-MS)	438:622	A facile and sensitive method based on high-performance liquid chromatography method of pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP) coupled with electrospray ionisation mass spectrometry (HPLC/ESI-MS) has been established for the analysis of the monosaccharide composition of polysaccharides in S. fusiforme.
24128572	5	28	dep	0.02	988:991	arg1	to					985:986	to	985:986	to	985:986	The LODs of the monosaccharides were in the range from 0.01 to 0.02 nmol.
24128572	2	29	theme	derivatization	498:511	arg1	PMP					550:552	PMP	550:552	PMP	550:552	A facile and sensitive method based on high-performance liquid chromatography method of pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP) coupled with electrospray ionisation mass spectrometry (HPLC/ESI-MS) has been established for the analysis of the monosaccharide composition of polysaccharides in S. fusiforme.
24128572	2	29	theme	derivatization	498:511	arg1	method					477:482	high-performance liquid chromatography method	438:482	high-performance liquid chromatography method of pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP) coupled with electrospray ionisation mass spectrometry (HPLC/ESI-MS)	438:622	A facile and sensitive method based on high-performance liquid chromatography method of pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP) coupled with electrospray ionisation mass spectrometry (HPLC/ESI-MS) has been established for the analysis of the monosaccharide composition of polysaccharides in S. fusiforme.
24128572	7	30	theme	solvent	1262:1268	arg1	acetate					1253:1259	20mM aqueous ammonium acetate	1231:1259	20mM aqueous ammonium acetate (solvent B) (pH 3.0) with Zorbax XDB-C18 column at 30 °C	1231:1316	The mobile phase of elution system was chosen as acetonitrile (solvent A) and 20mM aqueous ammonium acetate (solvent B) (pH 3.0) with Zorbax XDB-C18 column at 30 °C for the separation of the monosaccharide derivatives.
24128572	7	30	theme	solvent	1262:1268	arg1	B					1270:1270	solvent B	1262:1270	solvent B	1262:1270	The mobile phase of elution system was chosen as acetonitrile (solvent A) and 20mM aqueous ammonium acetate (solvent B) (pH 3.0) with Zorbax XDB-C18 column at 30 °C for the separation of the monosaccharide derivatives.
24128572	2	31	theme	electrospray	568:579	arg1	spectrometry					597:608	electrospray ionisation mass spectrometry	568:608	electrospray ionisation mass spectrometry (HPLC/ESI-MS)	568:622	A facile and sensitive method based on high-performance liquid chromatography method of pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP) coupled with electrospray ionisation mass spectrometry (HPLC/ESI-MS) has been established for the analysis of the monosaccharide composition of polysaccharides in S. fusiforme.
24128572	2	31	theme	electrospray	568:579	arg1	HPLC/ESI-MS					611:621	HPLC/ESI-MS	611:621	HPLC/ESI-MS	611:621	A facile and sensitive method based on high-performance liquid chromatography method of pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP) coupled with electrospray ionisation mass spectrometry (HPLC/ESI-MS) has been established for the analysis of the monosaccharide composition of polysaccharides in S. fusiforme.
24128572	3	32	theme	PMP-labelled	773:784	arg1	derivatives					786:796	PMP-labelled derivatives	773:796	PMP-labelled derivatives with aqueous ammonia	773:817	Monosaccharides have been converted into PMP-labelled derivatives with aqueous ammonia as a catalyst at 70 °C for 30 min.
24128572	0	33	theme	composition	31:41	arg1	Analysis					0:7	Analysis	0:7	Analysis of the monosaccharide composition of water-soluble polysaccharides from Sargassum	0:89	Analysis of the monosaccharide composition of water-soluble polysaccharides from Sargassum fusiforme by high performance liquid chromatography/electrospray ionisation mass spectrometry.
24128572	7	34	theme	derivatives	1359:1369	arg1	separation					1326:1335	the separation	1322:1335	the separation of the monosaccharide derivatives	1322:1369	The mobile phase of elution system was chosen as acetonitrile (solvent A) and 20mM aqueous ammonium acetate (solvent B) (pH 3.0) with Zorbax XDB-C18 column at 30 °C for the separation of the monosaccharide derivatives.
24128572	2	35	from	fusiforme	721:729	arg1	composition					684:694	the monosaccharide composition	665:694	the monosaccharide composition of polysaccharides in S. fusiforme	665:729	A facile and sensitive method based on high-performance liquid chromatography method of pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP) coupled with electrospray ionisation mass spectrometry (HPLC/ESI-MS) has been established for the analysis of the monosaccharide composition of polysaccharides in S. fusiforme.
24128572	2	36	theme	facile	401:406	arg1	method					422:427	A facile and sensitive method	399:427	A facile and sensitive method based on high-performance liquid chromatography method of pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP) coupled with electrospray ionisation mass spectrometry (HPLC/ESI-MS)	399:622	A facile and sensitive method based on high-performance liquid chromatography method of pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP) coupled with electrospray ionisation mass spectrometry (HPLC/ESI-MS) has been established for the analysis of the monosaccharide composition of polysaccharides in S. fusiforme.
24128572	0	37	theme	water-soluble	46:58	arg1	polysaccharides					60:74	water-soluble polysaccharides	46:74	water-soluble polysaccharides from Sargassum	46:89	Analysis of the monosaccharide composition of water-soluble polysaccharides from Sargassum fusiforme by high performance liquid chromatography/electrospray ionisation mass spectrometry.
24128572	8	38	theme	1-phenyl-3-methyl-5-pyrazolone	1517:1546	arg1	monosaccharides					1563:1577	1-phenyl-3-methyl-5-pyrazolone (PMP) labelled monosaccharides	1517:1577	1-phenyl-3-methyl-5-pyrazolone (PMP) labelled monosaccharides	1517:1577	Identification of the monosaccharides composition was carried out by analysis with mass spectral behaviour and chromatography characteristics of 1-phenyl-3-methyl-5-pyrazolone (PMP) labelled monosaccharides.
24128572	8	39	theme	PMP	1549:1551	arg1	monosaccharides					1563:1577	1-phenyl-3-methyl-5-pyrazolone (PMP) labelled monosaccharides	1517:1577	1-phenyl-3-methyl-5-pyrazolone (PMP) labelled monosaccharides	1517:1577	Identification of the monosaccharides composition was carried out by analysis with mass spectral behaviour and chromatography characteristics of 1-phenyl-3-methyl-5-pyrazolone (PMP) labelled monosaccharides.
24128572	2	40	theme	sensitive	412:420	arg1	method					422:427	A facile and sensitive method	399:427	A facile and sensitive method based on high-performance liquid chromatography method of pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP) coupled with electrospray ionisation mass spectrometry (HPLC/ESI-MS)	399:622	A facile and sensitive method based on high-performance liquid chromatography method of pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP) coupled with electrospray ionisation mass spectrometry (HPLC/ESI-MS) has been established for the analysis of the monosaccharide composition of polysaccharides in S. fusiforme.
24128572	7	41	theme	solvent	1216:1222	arg1	acetonitrile					1202:1213	acetonitrile	1202:1213	acetonitrile (solvent A)	1202:1225	The mobile phase of elution system was chosen as acetonitrile (solvent A) and 20mM aqueous ammonium acetate (solvent B) (pH 3.0) with Zorbax XDB-C18 column at 30 °C for the separation of the monosaccharide derivatives.
24128572	7	41	theme	solvent	1216:1222	arg1	A					1224:1224	solvent A	1216:1224	solvent A	1216:1224	The mobile phase of elution system was chosen as acetonitrile (solvent A) and 20mM aqueous ammonium acetate (solvent B) (pH 3.0) with Zorbax XDB-C18 column at 30 °C for the separation of the monosaccharide derivatives.
24128572	10	42	theme	uronic	1879:1884	arg1	acids					1886:1890	both uronic acids	1874:1890	both uronic acids	1874:1890	The result showed that the S. fusiforme polysaccharide consisted of mannose, glucose, galactose, xylose, fucose and glucuronic acid or galacturonic acid, or both uronic acids.
24128572	1	43	theme	interesting	297:307	arg1	bioactivities					309:321	interesting bioactivities	297:321	interesting bioactivities like antitumor, antioxidant, antimicrobial and immunomodulatory activities	297:396	Sargassum fusiforme (hijiki) is the well-known edible algae, whose polysaccharides have been proved to possess interesting bioactivities like antitumor, antioxidant, antimicrobial and immunomodulatory activities.
24128572	6	44	theme	PMP-labelled	999:1010	arg1	mixture					1012:1018	PMP-labelled mixture	999:1018	PMP-labelled mixture of monosaccharides	999:1037	PMP-labelled mixture of monosaccharides has been well separated by a reverse-phase HPLC and detected by on-line ESI-MS method under optimised conditions.
24128572	7	45	from	°C	1315:1316	arg1	A					1224:1224	solvent A	1216:1224	solvent A	1216:1224	The mobile phase of elution system was chosen as acetonitrile (solvent A) and 20mM aqueous ammonium acetate (solvent B) (pH 3.0) with Zorbax XDB-C18 column at 30 °C for the separation of the monosaccharide derivatives.
24128572	7	45	from	°C	1315:1316	arg1	phase					1164:1168	The mobile phase	1153:1168	The mobile phase of elution system	1153:1186	The mobile phase of elution system was chosen as acetonitrile (solvent A) and 20mM aqueous ammonium acetate (solvent B) (pH 3.0) with Zorbax XDB-C18 column at 30 °C for the separation of the monosaccharide derivatives.
24128572	7	45	from	°C	1315:1316	arg1	B					1270:1270	solvent B	1262:1270	solvent B	1262:1270	The mobile phase of elution system was chosen as acetonitrile (solvent A) and 20mM aqueous ammonium acetate (solvent B) (pH 3.0) with Zorbax XDB-C18 column at 30 °C for the separation of the monosaccharide derivatives.
24128572	7	45	from	°C	1315:1316	arg1	acetonitrile					1202:1213	acetonitrile	1202:1213	acetonitrile (solvent A)	1202:1225	The mobile phase of elution system was chosen as acetonitrile (solvent A) and 20mM aqueous ammonium acetate (solvent B) (pH 3.0) with Zorbax XDB-C18 column at 30 °C for the separation of the monosaccharide derivatives.
24128572	7	45	from	°C	1315:1316	arg1	acetate					1253:1259	20mM aqueous ammonium acetate	1231:1259	20mM aqueous ammonium acetate (solvent B) (pH 3.0) with Zorbax XDB-C18 column at 30 °C	1231:1316	The mobile phase of elution system was chosen as acetonitrile (solvent A) and 20mM aqueous ammonium acetate (solvent B) (pH 3.0) with Zorbax XDB-C18 column at 30 °C for the separation of the monosaccharide derivatives.
24128572	7	45	from	°C	1315:1316	arg1	pH					1274:1275	pH 3.0	1274:1279	pH 3.0	1274:1279	The mobile phase of elution system was chosen as acetonitrile (solvent A) and 20mM aqueous ammonium acetate (solvent B) (pH 3.0) with Zorbax XDB-C18 column at 30 °C for the separation of the monosaccharide derivatives.
24128572	10	46	theme	glucuronic	1833:1842	arg1	acid					1844:1847	glucuronic acid	1833:1847	glucuronic acid	1833:1847	The result showed that the S. fusiforme polysaccharide consisted of mannose, glucose, galactose, xylose, fucose and glucuronic acid or galacturonic acid, or both uronic acids.
24128572	7	47	theme	aqueous	1236:1242	arg1	acetate					1253:1259	20mM aqueous ammonium acetate	1231:1259	20mM aqueous ammonium acetate (solvent B) (pH 3.0) with Zorbax XDB-C18 column at 30 °C	1231:1316	The mobile phase of elution system was chosen as acetonitrile (solvent A) and 20mM aqueous ammonium acetate (solvent B) (pH 3.0) with Zorbax XDB-C18 column at 30 °C for the separation of the monosaccharide derivatives.
24128572	7	47	theme	aqueous	1236:1242	arg1	B					1270:1270	solvent B	1262:1270	solvent B	1262:1270	The mobile phase of elution system was chosen as acetonitrile (solvent A) and 20mM aqueous ammonium acetate (solvent B) (pH 3.0) with Zorbax XDB-C18 column at 30 °C for the separation of the monosaccharide derivatives.
24128572	7	47	theme	aqueous	1236:1242	arg1	pH					1274:1275	pH 3.0	1274:1279	pH 3.0	1274:1279	The mobile phase of elution system was chosen as acetonitrile (solvent A) and 20mM aqueous ammonium acetate (solvent B) (pH 3.0) with Zorbax XDB-C18 column at 30 °C for the separation of the monosaccharide derivatives.
24128572	7	47	theme	aqueous	1236:1242	arg1	phase					1164:1168	The mobile phase	1153:1168	The mobile phase of elution system	1153:1186	The mobile phase of elution system was chosen as acetonitrile (solvent A) and 20mM aqueous ammonium acetate (solvent B) (pH 3.0) with Zorbax XDB-C18 column at 30 °C for the separation of the monosaccharide derivatives.
24128572	2	48	with	derivatization	498:511	arg1	1-phenyl-3-methyl-5-pyrazolone					518:547	1-phenyl-3-methyl-5-pyrazolone	518:547	1-phenyl-3-methyl-5-pyrazolone	518:547	A facile and sensitive method based on high-performance liquid chromatography method of pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP) coupled with electrospray ionisation mass spectrometry (HPLC/ESI-MS) has been established for the analysis of the monosaccharide composition of polysaccharides in S. fusiforme.
24128572	7	49	with	acetate	1253:1259	arg1	column					1302:1307	Zorbax XDB-C18 column	1287:1307	Zorbax XDB-C18 column	1287:1307	The mobile phase of elution system was chosen as acetonitrile (solvent A) and 20mM aqueous ammonium acetate (solvent B) (pH 3.0) with Zorbax XDB-C18 column at 30 °C for the separation of the monosaccharide derivatives.
24128572	7	50	theme	system	1181:1186	arg1	acetate					1253:1259	20mM aqueous ammonium acetate	1231:1259	20mM aqueous ammonium acetate (solvent B) (pH 3.0) with Zorbax XDB-C18 column at 30 °C	1231:1316	The mobile phase of elution system was chosen as acetonitrile (solvent A) and 20mM aqueous ammonium acetate (solvent B) (pH 3.0) with Zorbax XDB-C18 column at 30 °C for the separation of the monosaccharide derivatives.
24128572	7	50	theme	system	1181:1186	arg1	acetonitrile					1202:1213	acetonitrile	1202:1213	acetonitrile (solvent A)	1202:1225	The mobile phase of elution system was chosen as acetonitrile (solvent A) and 20mM aqueous ammonium acetate (solvent B) (pH 3.0) with Zorbax XDB-C18 column at 30 °C for the separation of the monosaccharide derivatives.
24128572	7	50	theme	system	1181:1186	arg1	phase					1164:1168	The mobile phase	1153:1168	The mobile phase of elution system	1153:1186	The mobile phase of elution system was chosen as acetonitrile (solvent A) and 20mM aqueous ammonium acetate (solvent B) (pH 3.0) with Zorbax XDB-C18 column at 30 °C for the separation of the monosaccharide derivatives.
24128572	5	51	theme	monosaccharides	941:955	arg1	LODs					929:932	The LODs	925:932	The LODs of the monosaccharides	925:955	The LODs of the monosaccharides were in the range from 0.01 to 0.02 nmol.
24128572	6	52	theme	reverse-phase	1068:1080	arg1	HPLC					1082:1085	a reverse-phase HPLC	1066:1085	a reverse-phase HPLC	1066:1085	PMP-labelled mixture of monosaccharides has been well separated by a reverse-phase HPLC and detected by on-line ESI-MS method under optimised conditions.
24128572	8	53	theme	labelled	1554:1561	arg1	monosaccharides					1563:1577	1-phenyl-3-methyl-5-pyrazolone (PMP) labelled monosaccharides	1517:1577	1-phenyl-3-methyl-5-pyrazolone (PMP) labelled monosaccharides	1517:1577	Identification of the monosaccharides composition was carried out by analysis with mass spectral behaviour and chromatography characteristics of 1-phenyl-3-methyl-5-pyrazolone (PMP) labelled monosaccharides.
24128572	0	54	from	Sargassum	81:89	arg1	composition					31:41	the monosaccharide composition	12:41	the monosaccharide composition of water-soluble polysaccharides from Sargassum	12:89	Analysis of the monosaccharide composition of water-soluble polysaccharides from Sargassum fusiforme by high performance liquid chromatography/electrospray ionisation mass spectrometry.
24128572	0	54	from	Sargassum	81:89	arg1	polysaccharides					60:74	water-soluble polysaccharides	46:74	water-soluble polysaccharides from Sargassum	46:89	Analysis of the monosaccharide composition of water-soluble polysaccharides from Sargassum fusiforme by high performance liquid chromatography/electrospray ionisation mass spectrometry.
24128572	10	55	theme	fusiforme	1747:1755	arg1	polysaccharide					1757:1770	the S. fusiforme polysaccharide	1740:1770	the S. fusiforme polysaccharide	1740:1770	The result showed that the S. fusiforme polysaccharide consisted of mannose, glucose, galactose, xylose, fucose and glucuronic acid or galacturonic acid, or both uronic acids.
24128572	7	56	theme	mobile	1157:1162	arg1	acetate					1253:1259	20mM aqueous ammonium acetate	1231:1259	20mM aqueous ammonium acetate (solvent B) (pH 3.0) with Zorbax XDB-C18 column at 30 °C	1231:1316	The mobile phase of elution system was chosen as acetonitrile (solvent A) and 20mM aqueous ammonium acetate (solvent B) (pH 3.0) with Zorbax XDB-C18 column at 30 °C for the separation of the monosaccharide derivatives.
24128572	7	56	theme	mobile	1157:1162	arg1	acetonitrile					1202:1213	acetonitrile	1202:1213	acetonitrile (solvent A)	1202:1225	The mobile phase of elution system was chosen as acetonitrile (solvent A) and 20mM aqueous ammonium acetate (solvent B) (pH 3.0) with Zorbax XDB-C18 column at 30 °C for the separation of the monosaccharide derivatives.
24128572	7	56	theme	mobile	1157:1162	arg1	phase					1164:1168	The mobile phase	1153:1168	The mobile phase of elution system	1153:1186	The mobile phase of elution system was chosen as acetonitrile (solvent A) and 20mM aqueous ammonium acetate (solvent B) (pH 3.0) with Zorbax XDB-C18 column at 30 °C for the separation of the monosaccharide derivatives.
24128572	9	57	theme	high	1617:1620	arg1	stabilities					1631:1641	high chemical stabilities	1617:1641	high chemical stabilities	1617:1641	All PMP-labelled derivatives display high chemical stabilities, whose regular MS fragmentation is specific for reducing labelled sugars.
24128572	0	58	theme	mass	167:170	arg1	spectrometry					172:183	high performance liquid chromatography/electrospray ionisation mass spectrometry	104:183	high performance liquid chromatography/electrospray ionisation mass spectrometry	104:183	Analysis of the monosaccharide composition of water-soluble polysaccharides from Sargassum fusiforme by high performance liquid chromatography/electrospray ionisation mass spectrometry.
24128572	7	59	theme	XDB-C18	1294:1300	arg1	column					1302:1307	Zorbax XDB-C18 column	1287:1307	Zorbax XDB-C18 column	1287:1307	The mobile phase of elution system was chosen as acetonitrile (solvent A) and 20mM aqueous ammonium acetate (solvent B) (pH 3.0) with Zorbax XDB-C18 column at 30 °C for the separation of the monosaccharide derivatives.
24128572	1	60	contain	possess	289:295	arg2	bioactivities					309:321	interesting bioactivities	297:321	interesting bioactivities like antitumor, antioxidant, antimicrobial and immunomodulatory activities	297:396	Sargassum fusiforme (hijiki) is the well-known edible algae, whose polysaccharides have been proved to possess interesting bioactivities like antitumor, antioxidant, antimicrobial and immunomodulatory activities.
24128572	1	60	contain	possess	289:295	arg1	polysaccharides					253:267	polysaccharides	253:267	polysaccharides	253:267	Sargassum fusiforme (hijiki) is the well-known edible algae, whose polysaccharides have been proved to possess interesting bioactivities like antitumor, antioxidant, antimicrobial and immunomodulatory activities.
24128572	1	61	theme	edible	233:238	arg1	algae					240:244	the well-known edible algae	218:244	the well-known edible algae	218:244	Sargassum fusiforme (hijiki) is the well-known edible algae, whose polysaccharides have been proved to possess interesting bioactivities like antitumor, antioxidant, antimicrobial and immunomodulatory activities.
24128572	1	61	theme	edible	233:238	arg1	fusiforme					196:204	Sargassum fusiforme	186:204	Sargassum fusiforme (hijiki)	186:213	Sargassum fusiforme (hijiki) is the well-known edible algae, whose polysaccharides have been proved to possess interesting bioactivities like antitumor, antioxidant, antimicrobial and immunomodulatory activities.
24128572	1	62	theme	Sargassum	186:194	arg1	algae					240:244	the well-known edible algae	218:244	the well-known edible algae	218:244	Sargassum fusiforme (hijiki) is the well-known edible algae, whose polysaccharides have been proved to possess interesting bioactivities like antitumor, antioxidant, antimicrobial and immunomodulatory activities.
24128572	1	62	theme	Sargassum	186:194	arg1	fusiforme					196:204	Sargassum fusiforme	186:204	Sargassum fusiforme (hijiki)	186:213	Sargassum fusiforme (hijiki) is the well-known edible algae, whose polysaccharides have been proved to possess interesting bioactivities like antitumor, antioxidant, antimicrobial and immunomodulatory activities.
24128572	1	62	theme	Sargassum	186:194	arg1	hijiki					207:212	hijiki	207:212	hijiki	207:212	Sargassum fusiforme (hijiki) is the well-known edible algae, whose polysaccharides have been proved to possess interesting bioactivities like antitumor, antioxidant, antimicrobial and immunomodulatory activities.
24128572	9	63	theme	MS	1658:1659	arg1	fragmentation					1661:1673	fragmentation	1661:1673	fragmentation	1661:1673	All PMP-labelled derivatives display high chemical stabilities, whose regular MS fragmentation is specific for reducing labelled sugars.
24128572	6	64	theme	ESI-MS	1111:1116	arg1	method					1118:1123	on-line ESI-MS method	1103:1123	on-line ESI-MS method	1103:1123	PMP-labelled mixture of monosaccharides has been well separated by a reverse-phase HPLC and detected by on-line ESI-MS method under optimised conditions.
24128572	2	65	theme	ionisation	581:590	arg1	spectrometry					597:608	electrospray ionisation mass spectrometry	568:608	electrospray ionisation mass spectrometry (HPLC/ESI-MS)	568:622	A facile and sensitive method based on high-performance liquid chromatography method of pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP) coupled with electrospray ionisation mass spectrometry (HPLC/ESI-MS) has been established for the analysis of the monosaccharide composition of polysaccharides in S. fusiforme.
24128572	2	65	theme	ionisation	581:590	arg1	HPLC/ESI-MS					611:621	HPLC/ESI-MS	611:621	HPLC/ESI-MS	611:621	A facile and sensitive method based on high-performance liquid chromatography method of pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP) coupled with electrospray ionisation mass spectrometry (HPLC/ESI-MS) has been established for the analysis of the monosaccharide composition of polysaccharides in S. fusiforme.
24128572	8	66	theme	monosaccharides	1394:1408	arg1	composition					1410:1420	the monosaccharides composition	1390:1420	the monosaccharides composition	1390:1420	Identification of the monosaccharides composition was carried out by analysis with mass spectral behaviour and chromatography characteristics of 1-phenyl-3-methyl-5-pyrazolone (PMP) labelled monosaccharides.
24128572	0	67	theme	performance	109:119	arg1	spectrometry					172:183	high performance liquid chromatography/electrospray ionisation mass spectrometry	104:183	high performance liquid chromatography/electrospray ionisation mass spectrometry	104:183	Analysis of the monosaccharide composition of water-soluble polysaccharides from Sargassum fusiforme by high performance liquid chromatography/electrospray ionisation mass spectrometry.
24128572	8	68	with	analysis	1441:1448	arg1	behaviour					1469:1477	mass spectral behaviour	1455:1477	mass spectral behaviour	1455:1477	Identification of the monosaccharides composition was carried out by analysis with mass spectral behaviour and chromatography characteristics of 1-phenyl-3-methyl-5-pyrazolone (PMP) labelled monosaccharides.
24128572	8	68	with	analysis	1441:1448	arg1	characteristics					1498:1512	chromatography characteristics	1483:1512	chromatography characteristics	1483:1512	Identification of the monosaccharides composition was carried out by analysis with mass spectral behaviour and chromatography characteristics of 1-phenyl-3-methyl-5-pyrazolone (PMP) labelled monosaccharides.
24128572	0	69	theme	chromatography/electrospray	128:154	arg1	spectrometry					172:183	high performance liquid chromatography/electrospray ionisation mass spectrometry	104:183	high performance liquid chromatography/electrospray ionisation mass spectrometry	104:183	Analysis of the monosaccharide composition of water-soluble polysaccharides from Sargassum fusiforme by high performance liquid chromatography/electrospray ionisation mass spectrometry.
24128572	5	70	from	nmol	993:996	arg1	range					969:973	the range	965:973	the range from 0.01 to 0.02 nmol	965:996	The LODs of the monosaccharides were in the range from 0.01 to 0.02 nmol.
24128572	4	71	theme	pre-column	878:887	arg1	process					904:910	the pre-column derivatization process	874:910	the pre-column derivatization process	874:910	The optimisation of the pre-column derivatization process was studied.
24128572	2	72	theme	liquid	455:460	arg1	chromatography					462:475	high-performance liquid chromatography	438:475	high-performance liquid chromatography method of pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP) coupled with electrospray ionisation mass spectrometry (HPLC/ESI-MS)	438:622	A facile and sensitive method based on high-performance liquid chromatography method of pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP) coupled with electrospray ionisation mass spectrometry (HPLC/ESI-MS) has been established for the analysis of the monosaccharide composition of polysaccharides in S. fusiforme.
24128572	9	73	theme	labelled	1700:1707	arg1	sugars					1709:1714	labelled sugars	1700:1714	labelled sugars	1700:1714	All PMP-labelled derivatives display high chemical stabilities, whose regular MS fragmentation is specific for reducing labelled sugars.
24128572	2	74	from	polysaccharides	699:713	arg1	fusiforme					721:729	S. fusiforme	718:729	S. fusiforme	718:729	A facile and sensitive method based on high-performance liquid chromatography method of pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP) coupled with electrospray ionisation mass spectrometry (HPLC/ESI-MS) has been established for the analysis of the monosaccharide composition of polysaccharides in S. fusiforme.
24128572	0	75	theme	monosaccharide	16:29	arg1	composition					31:41	the monosaccharide composition	12:41	the monosaccharide composition of water-soluble polysaccharides from Sargassum	12:89	Analysis of the monosaccharide composition of water-soluble polysaccharides from Sargassum fusiforme by high performance liquid chromatography/electrospray ionisation mass spectrometry.
24128572	1	76	theme	antitumor	328:336	arg1	activities					387:396	antitumor, antioxidant, antimicrobial and immunomodulatory activities	328:396	antitumor, antioxidant, antimicrobial and immunomodulatory activities	328:396	Sargassum fusiforme (hijiki) is the well-known edible algae, whose polysaccharides have been proved to possess interesting bioactivities like antitumor, antioxidant, antimicrobial and immunomodulatory activities.
24128572	8	77	theme	mass	1455:1458	arg1	behaviour					1469:1477	mass spectral behaviour	1455:1477	mass spectral behaviour	1455:1477	Identification of the monosaccharides composition was carried out by analysis with mass spectral behaviour and chromatography characteristics of 1-phenyl-3-methyl-5-pyrazolone (PMP) labelled monosaccharides.
24128572	2	78	theme	pre-column	487:496	arg1	derivatization					498:511	pre-column derivatization	487:511	pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone	487:547	A facile and sensitive method based on high-performance liquid chromatography method of pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP) coupled with electrospray ionisation mass spectrometry (HPLC/ESI-MS) has been established for the analysis of the monosaccharide composition of polysaccharides in S. fusiforme.
24128572	7	79	theme	ammonium	1244:1251	arg1	acetate					1253:1259	20mM aqueous ammonium acetate	1231:1259	20mM aqueous ammonium acetate (solvent B) (pH 3.0) with Zorbax XDB-C18 column at 30 °C	1231:1316	The mobile phase of elution system was chosen as acetonitrile (solvent A) and 20mM aqueous ammonium acetate (solvent B) (pH 3.0) with Zorbax XDB-C18 column at 30 °C for the separation of the monosaccharide derivatives.
24128572	7	79	theme	ammonium	1244:1251	arg1	B					1270:1270	solvent B	1262:1270	solvent B	1262:1270	The mobile phase of elution system was chosen as acetonitrile (solvent A) and 20mM aqueous ammonium acetate (solvent B) (pH 3.0) with Zorbax XDB-C18 column at 30 °C for the separation of the monosaccharide derivatives.
24128572	7	79	theme	ammonium	1244:1251	arg1	pH					1274:1275	pH 3.0	1274:1279	pH 3.0	1274:1279	The mobile phase of elution system was chosen as acetonitrile (solvent A) and 20mM aqueous ammonium acetate (solvent B) (pH 3.0) with Zorbax XDB-C18 column at 30 °C for the separation of the monosaccharide derivatives.
24128572	7	79	theme	ammonium	1244:1251	arg1	phase					1164:1168	The mobile phase	1153:1168	The mobile phase of elution system	1153:1186	The mobile phase of elution system was chosen as acetonitrile (solvent A) and 20mM aqueous ammonium acetate (solvent B) (pH 3.0) with Zorbax XDB-C18 column at 30 °C for the separation of the monosaccharide derivatives.
24128572	1	80	theme	antioxidant	339:349	arg1	activities					387:396	antitumor, antioxidant, antimicrobial and immunomodulatory activities	328:396	antitumor, antioxidant, antimicrobial and immunomodulatory activities	328:396	Sargassum fusiforme (hijiki) is the well-known edible algae, whose polysaccharides have been proved to possess interesting bioactivities like antitumor, antioxidant, antimicrobial and immunomodulatory activities.
24128572	7	81	theme	monosaccharide	1344:1357	arg1	derivatives					1359:1369	the monosaccharide derivatives	1340:1369	the monosaccharide derivatives	1340:1369	The mobile phase of elution system was chosen as acetonitrile (solvent A) and 20mM aqueous ammonium acetate (solvent B) (pH 3.0) with Zorbax XDB-C18 column at 30 °C for the separation of the monosaccharide derivatives.
24128572	0	82	theme	polysaccharides	60:74	arg1	composition					31:41	the monosaccharide composition	12:41	the monosaccharide composition of water-soluble polysaccharides from Sargassum	12:89	Analysis of the monosaccharide composition of water-soluble polysaccharides from Sargassum fusiforme by high performance liquid chromatography/electrospray ionisation mass spectrometry.
24128572	1	83	theme	antimicrobial	352:364	arg1	activities					387:396	antitumor, antioxidant, antimicrobial and immunomodulatory activities	328:396	antitumor, antioxidant, antimicrobial and immunomodulatory activities	328:396	Sargassum fusiforme (hijiki) is the well-known edible algae, whose polysaccharides have been proved to possess interesting bioactivities like antitumor, antioxidant, antimicrobial and immunomodulatory activities.
24128572	3	84	theme	aqueous	803:809	arg1	ammonia					811:817	aqueous ammonia	803:817	aqueous ammonia	803:817	Monosaccharides have been converted into PMP-labelled derivatives with aqueous ammonia as a catalyst at 70 °C for 30 min.
24128572	8	85	theme	chromatography	1483:1496	arg1	characteristics					1498:1512	chromatography characteristics	1483:1512	chromatography characteristics	1483:1512	Identification of the monosaccharides composition was carried out by analysis with mass spectral behaviour and chromatography characteristics of 1-phenyl-3-methyl-5-pyrazolone (PMP) labelled monosaccharides.
24128572	1	86	theme	immunomodulatory	370:385	arg1	activities					387:396	antitumor, antioxidant, antimicrobial and immunomodulatory activities	328:396	antitumor, antioxidant, antimicrobial and immunomodulatory activities	328:396	Sargassum fusiforme (hijiki) is the well-known edible algae, whose polysaccharides have been proved to possess interesting bioactivities like antitumor, antioxidant, antimicrobial and immunomodulatory activities.
24128572	7	87	with	acetonitrile	1202:1213	arg1	column					1302:1307	Zorbax XDB-C18 column	1287:1307	Zorbax XDB-C18 column	1287:1307	The mobile phase of elution system was chosen as acetonitrile (solvent A) and 20mM aqueous ammonium acetate (solvent B) (pH 3.0) with Zorbax XDB-C18 column at 30 °C for the separation of the monosaccharide derivatives.
24128572	4	88	theme	process	904:910	arg1	optimisation					858:869	The optimisation	854:869	The optimisation of the pre-column derivatization process	854:910	The optimisation of the pre-column derivatization process was studied.
24128572	10	89	theme	galacturonic	1852:1863	arg1	acid					1865:1868	galacturonic acid	1852:1868	galacturonic acid	1852:1868	The result showed that the S. fusiforme polysaccharide consisted of mannose, glucose, galactose, xylose, fucose and glucuronic acid or galacturonic acid, or both uronic acids.
25035517	5	0	theme	colonization	779:790	arg1	genes					792:796	the identified colonization genes	764:796	the identified colonization genes	764:796	From the identified colonization genes, epaX encodes a glycosyltransferase located in a variable region of the enterococcal polysaccharide antigen (epa) locus.
25035517	5	1	theme	locus	912:916	arg1	region					856:861	a variable region	845:861	a variable region of the enterococcal polysaccharide antigen (epa) locus	845:916	From the identified colonization genes, epaX encodes a glycosyltransferase located in a variable region of the enterococcal polysaccharide antigen (epa) locus.
25035517	6	2	theme	cell	1011:1014	arg1	integrity					1021:1029	cell wall integrity	1011:1029	cell wall integrity	1011:1029	We demonstrated that EpaX acts on sugar composition, promoting resistance to bile salts and cell wall integrity.
25035517	1	3	theme	human	165:169	arg1	intestine					171:179	the human intestine	161:179	the human intestine	161:179	Enterococcus faecalis is a commensal bacterium of the human intestine and a major opportunistic pathogen in immunocompromised and elderly patients.
25035517	0	4	theme	colonization	97:108	arg1	determinant					71:81	a key determinant	65:81	a key determinant of intestinal colonization	65:108	The surface rhamnopolysaccharide epa of Enterococcus faecalis is a key determinant of intestinal colonization.
25035517	0	4	theme	colonization	97:108	arg1	epa					33:35	The surface rhamnopolysaccharide epa	0:35	The surface rhamnopolysaccharide epa of Enterococcus faecalis	0:60	The surface rhamnopolysaccharide epa of Enterococcus faecalis is a key determinant of intestinal colonization.
25035517	0	5	theme	intestinal	86:95	arg1	colonization					97:108	intestinal colonization	86:108	intestinal colonization	86:108	The surface rhamnopolysaccharide epa of Enterococcus faecalis is a key determinant of intestinal colonization.
25035517	1	6	from	bacterium	148:156	arg1	patients					249:256	immunocompromised and elderly patients	219:256	patients	249:256	Enterococcus faecalis is a commensal bacterium of the human intestine and a major opportunistic pathogen in immunocompromised and elderly patients.
25035517	5	7	theme	enterococcal	870:881	arg1	epa					907:909	epa	907:909	epa	907:909	From the identified colonization genes, epaX encodes a glycosyltransferase located in a variable region of the enterococcal polysaccharide antigen (epa) locus.
25035517	5	7	theme	enterococcal	870:881	arg1	antigen					898:904	enterococcal polysaccharide antigen	870:904	the enterococcal polysaccharide antigen (epa) locus	866:916	From the identified colonization genes, epaX encodes a glycosyltransferase located in a variable region of the enterococcal polysaccharide antigen (epa) locus.
25035517	7	8	theme	intestinal	1187:1196	arg1	colonization					1198:1209	enterococcal intestinal colonization	1174:1209	enterococcal intestinal colonization	1174:1209	Given that EpaX is enriched in hospital-adapted isolates, this study points to the importance of the epa variability as a key determinant for enterococcal intestinal colonization.
25035517	2	9	theme	infection	291:299	arg1	pathogenesis					263:274	The pathogenesis	259:274	The pathogenesis of E. faecalis infection	259:299	The pathogenesis of E. faecalis infection relies in part on its capacity to colonize the gut.
25035517	1	10	theme	intestine	171:179	arg1	pathogen					207:214	a major opportunistic pathogen	185:214	a major opportunistic pathogen in immunocompromised and elderly patients	185:256	Enterococcus faecalis is a commensal bacterium of the human intestine and a major opportunistic pathogen in immunocompromised and elderly patients.
25035517	1	10	theme	intestine	171:179	arg1	faecalis					124:131	Enterococcus faecalis	111:131	Enterococcus faecalis	111:131	Enterococcus faecalis is a commensal bacterium of the human intestine and a major opportunistic pathogen in immunocompromised and elderly patients.
25035517	1	10	theme	intestine	171:179	arg1	bacterium					148:156	a commensal bacterium	136:156	a commensal bacterium of the human intestine	136:179	Enterococcus faecalis is a commensal bacterium of the human intestine and a major opportunistic pathogen in immunocompromised and elderly patients.
25035517	6	11	theme	bile	996:999	arg1	salts					1001:1005	bile salts	996:1005	bile salts	996:1005	We demonstrated that EpaX acts on sugar composition, promoting resistance to bile salts and cell wall integrity.
25035517	2	12	theme	faecalis	282:289	arg1	infection					291:299	E. faecalis infection	279:299	E. faecalis infection	279:299	The pathogenesis of E. faecalis infection relies in part on its capacity to colonize the gut.
25035517	4	13	theme	related	655:661	arg1	phenotypes					644:653	the following phenotypes	630:653	the following phenotypes related to intestinal lifestyle	630:685	To identify and characterize E. faecalis genes that are key to intestinal colonization, our strategy consisted in screening mutants for the following phenotypes related to intestinal lifestyle: antibiotic resistance, overgrowth, and competition against microbiota.
25035517	0	14	theme	surface	4:10	arg1	determinant					71:81	a key determinant	65:81	a key determinant of intestinal colonization	65:108	The surface rhamnopolysaccharide epa of Enterococcus faecalis is a key determinant of intestinal colonization.
25035517	0	14	theme	surface	4:10	arg1	epa					33:35	The surface rhamnopolysaccharide epa	0:35	The surface rhamnopolysaccharide epa of Enterococcus faecalis	0:60	The surface rhamnopolysaccharide epa of Enterococcus faecalis is a key determinant of intestinal colonization.
25035517	5	15	from	located	834:840	arg1	region					856:861	a variable region	845:861	a variable region of the enterococcal polysaccharide antigen (epa) locus	845:916	From the identified colonization genes, epaX encodes a glycosyltransferase located in a variable region of the enterococcal polysaccharide antigen (epa) locus.
25035517	5	16	theme	antigen	898:904	arg1	locus					912:916	the enterococcal polysaccharide antigen (epa) locus	866:916	the enterococcal polysaccharide antigen (epa) locus	866:916	From the identified colonization genes, epaX encodes a glycosyltransferase located in a variable region of the enterococcal polysaccharide antigen (epa) locus.
25035517	5	17	theme	located	834:840	arg1	epaX					799:802	epaX	799:802	epaX	799:802	From the identified colonization genes, epaX encodes a glycosyltransferase located in a variable region of the enterococcal polysaccharide antigen (epa) locus.
25035517	5	17	theme	located	834:840	arg1	glycosyltransferase					814:832	a glycosyltransferase	812:832	a glycosyltransferase located in a variable region of the enterococcal polysaccharide antigen (epa) locus	812:916	From the identified colonization genes, epaX encodes a glycosyltransferase located in a variable region of the enterococcal polysaccharide antigen (epa) locus.
25035517	1	18	theme	major	187:191	arg1	pathogen					207:214	a major opportunistic pathogen	185:214	a major opportunistic pathogen in immunocompromised and elderly patients	185:256	Enterococcus faecalis is a commensal bacterium of the human intestine and a major opportunistic pathogen in immunocompromised and elderly patients.
25035517	3	19	theme	intestinal	377:386	arg1	homeostasis					388:398	intestinal homeostasis	377:398	intestinal homeostasis	377:398	Following disruption of intestinal homeostasis, E. faecalis can overgrow, cross the intestinal barrier, and enter the lymph and bloodstream.
25035517	1	20	theme	opportunistic	193:205	arg1	pathogen					207:214	a major opportunistic pathogen	185:214	a major opportunistic pathogen in immunocompromised and elderly patients	185:256	Enterococcus faecalis is a commensal bacterium of the human intestine and a major opportunistic pathogen in immunocompromised and elderly patients.
25035517	3	21	theme	homeostasis	388:398	arg1	disruption					363:372	disruption	363:372	disruption of intestinal homeostasis	363:398	Following disruption of intestinal homeostasis, E. faecalis can overgrow, cross the intestinal barrier, and enter the lymph and bloodstream.
25035517	4	22	theme	intestinal	557:566	arg1	colonization					568:579	intestinal colonization	557:579	intestinal colonization	557:579	To identify and characterize E. faecalis genes that are key to intestinal colonization, our strategy consisted in screening mutants for the following phenotypes related to intestinal lifestyle: antibiotic resistance, overgrowth, and competition against microbiota.
25035517	1	23	theme	Enterococcus	111:122	arg1	faecalis					124:131	Enterococcus faecalis	111:131	Enterococcus faecalis	111:131	Enterococcus faecalis is a commensal bacterium of the human intestine and a major opportunistic pathogen in immunocompromised and elderly patients.
25035517	1	23	theme	Enterococcus	111:122	arg1	bacterium					148:156	a commensal bacterium	136:156	a commensal bacterium of the human intestine	136:179	Enterococcus faecalis is a commensal bacterium of the human intestine and a major opportunistic pathogen in immunocompromised and elderly patients.
25035517	0	24	theme	Enterococcus	40:51	arg1	faecalis					53:60	Enterococcus faecalis	40:60	Enterococcus faecalis	40:60	The surface rhamnopolysaccharide epa of Enterococcus faecalis is a key determinant of intestinal colonization.
25035517	7	25	theme	enterococcal	1174:1185	arg1	colonization					1198:1209	enterococcal intestinal colonization	1174:1209	enterococcal intestinal colonization	1174:1209	Given that EpaX is enriched in hospital-adapted isolates, this study points to the importance of the epa variability as a key determinant for enterococcal intestinal colonization.
25035517	4	26	theme	intestinal	666:675	arg1	lifestyle					677:685	intestinal lifestyle	666:685	intestinal lifestyle	666:685	To identify and characterize E. faecalis genes that are key to intestinal colonization, our strategy consisted in screening mutants for the following phenotypes related to intestinal lifestyle: antibiotic resistance, overgrowth, and competition against microbiota.
25035517	3	27	theme	intestinal	437:446	arg1	barrier					448:454	the intestinal barrier	433:454	the intestinal barrier	433:454	Following disruption of intestinal homeostasis, E. faecalis can overgrow, cross the intestinal barrier, and enter the lymph and bloodstream.
25035517	4	28	theme	faecalis	526:533	arg1	genes					535:539	E. faecalis genes	523:539	E. faecalis genes that are key to intestinal colonization	523:579	To identify and characterize E. faecalis genes that are key to intestinal colonization, our strategy consisted in screening mutants for the following phenotypes related to intestinal lifestyle: antibiotic resistance, overgrowth, and competition against microbiota.
25035517	7	29	theme	hospital-adapted	1063:1078	arg1	isolates					1080:1087	hospital-adapted isolates	1063:1087	hospital-adapted isolates	1063:1087	Given that EpaX is enriched in hospital-adapted isolates, this study points to the importance of the epa variability as a key determinant for enterococcal intestinal colonization.
25035517	7	30	theme	key	1154:1156	arg1	determinant					1158:1168	a key determinant	1152:1168	a key determinant for enterococcal intestinal colonization	1152:1209	Given that EpaX is enriched in hospital-adapted isolates, this study points to the importance of the epa variability as a key determinant for enterococcal intestinal colonization.
25035517	1	31	theme	immunocompromised	219:235	arg1	patients					249:256	immunocompromised and elderly patients	219:256	patients	249:256	Enterococcus faecalis is a commensal bacterium of the human intestine and a major opportunistic pathogen in immunocompromised and elderly patients.
25035517	7	32	theme	epa	1133:1135	arg1	variability					1137:1147	the epa variability	1129:1147	the epa variability	1129:1147	Given that EpaX is enriched in hospital-adapted isolates, this study points to the importance of the epa variability as a key determinant for enterococcal intestinal colonization.
25035517	4	33	theme	E.	523:524	arg1	genes					535:539	E. faecalis genes	523:539	E. faecalis genes that are key to intestinal colonization	523:579	To identify and characterize E. faecalis genes that are key to intestinal colonization, our strategy consisted in screening mutants for the following phenotypes related to intestinal lifestyle: antibiotic resistance, overgrowth, and competition against microbiota.
25035517	0	34	theme	faecalis	53:60	arg1	determinant					71:81	a key determinant	65:81	a key determinant of intestinal colonization	65:108	The surface rhamnopolysaccharide epa of Enterococcus faecalis is a key determinant of intestinal colonization.
25035517	0	34	theme	faecalis	53:60	arg1	epa					33:35	The surface rhamnopolysaccharide epa	0:35	The surface rhamnopolysaccharide epa of Enterococcus faecalis	0:60	The surface rhamnopolysaccharide epa of Enterococcus faecalis is a key determinant of intestinal colonization.
25035517	4	35	dep	identify	497:504	arg1	resistance					699:708	antibiotic resistance	688:708	antibiotic resistance	688:708	To identify and characterize E. faecalis genes that are key to intestinal colonization, our strategy consisted in screening mutants for the following phenotypes related to intestinal lifestyle: antibiotic resistance, overgrowth, and competition against microbiota.
25035517	4	35	dep	identify	497:504	arg1	overgrowth					711:720	overgrowth	711:720	overgrowth	711:720	To identify and characterize E. faecalis genes that are key to intestinal colonization, our strategy consisted in screening mutants for the following phenotypes related to intestinal lifestyle: antibiotic resistance, overgrowth, and competition against microbiota.
25035517	4	35	dep	identify	497:504	arg1	competition					727:737	competition	727:737	competition	727:737	To identify and characterize E. faecalis genes that are key to intestinal colonization, our strategy consisted in screening mutants for the following phenotypes related to intestinal lifestyle: antibiotic resistance, overgrowth, and competition against microbiota.
25035517	7	36	theme	variability	1137:1147	arg1	importance					1115:1124	the importance	1111:1124	the importance of the epa variability	1111:1147	Given that EpaX is enriched in hospital-adapted isolates, this study points to the importance of the epa variability as a key determinant for enterococcal intestinal colonization.
25035517	2	37	theme	E.	279:280	arg1	infection					291:299	E. faecalis infection	279:299	E. faecalis infection	279:299	The pathogenesis of E. faecalis infection relies in part on its capacity to colonize the gut.
25035517	1	38	from	pathogen	207:214	arg1	patients					249:256	immunocompromised and elderly patients	219:256	patients	249:256	Enterococcus faecalis is a commensal bacterium of the human intestine and a major opportunistic pathogen in immunocompromised and elderly patients.
25035517	5	39	from	region	856:861	arg1	located					834:840	located	834:840	located	834:840	From the identified colonization genes, epaX encodes a glycosyltransferase located in a variable region of the enterococcal polysaccharide antigen (epa) locus.
25035517	1	40	theme	commensal	138:146	arg1	faecalis					124:131	Enterococcus faecalis	111:131	Enterococcus faecalis	111:131	Enterococcus faecalis is a commensal bacterium of the human intestine and a major opportunistic pathogen in immunocompromised and elderly patients.
25035517	1	40	theme	commensal	138:146	arg1	bacterium					148:156	a commensal bacterium	136:156	a commensal bacterium of the human intestine	136:179	Enterococcus faecalis is a commensal bacterium of the human intestine and a major opportunistic pathogen in immunocompromised and elderly patients.
25035517	1	41	theme	elderly	241:247	arg1	patients					249:256	immunocompromised and elderly patients	219:256	patients	249:256	Enterococcus faecalis is a commensal bacterium of the human intestine and a major opportunistic pathogen in immunocompromised and elderly patients.
25035517	0	42	theme	key	67:69	arg1	determinant					71:81	a key determinant	65:81	a key determinant of intestinal colonization	65:108	The surface rhamnopolysaccharide epa of Enterococcus faecalis is a key determinant of intestinal colonization.
25035517	0	42	theme	key	67:69	arg1	epa					33:35	The surface rhamnopolysaccharide epa	0:35	The surface rhamnopolysaccharide epa of Enterococcus faecalis	0:60	The surface rhamnopolysaccharide epa of Enterococcus faecalis is a key determinant of intestinal colonization.
25035517	4	43	theme	following	634:642	arg1	phenotypes					644:653	the following phenotypes	630:653	the following phenotypes related to intestinal lifestyle	630:685	To identify and characterize E. faecalis genes that are key to intestinal colonization, our strategy consisted in screening mutants for the following phenotypes related to intestinal lifestyle: antibiotic resistance, overgrowth, and competition against microbiota.
25035517	5	44	theme	variable	847:854	arg1	region					856:861	a variable region	845:861	a variable region of the enterococcal polysaccharide antigen (epa) locus	845:916	From the identified colonization genes, epaX encodes a glycosyltransferase located in a variable region of the enterococcal polysaccharide antigen (epa) locus.
25035517	4	45	theme	antibiotic	688:697	arg1	resistance					699:708	antibiotic resistance	688:708	antibiotic resistance	688:708	To identify and characterize E. faecalis genes that are key to intestinal colonization, our strategy consisted in screening mutants for the following phenotypes related to intestinal lifestyle: antibiotic resistance, overgrowth, and competition against microbiota.
25035517	5	46	theme	polysaccharide	883:896	arg1	epa					907:909	epa	907:909	epa	907:909	From the identified colonization genes, epaX encodes a glycosyltransferase located in a variable region of the enterococcal polysaccharide antigen (epa) locus.
25035517	5	46	theme	polysaccharide	883:896	arg1	antigen					898:904	enterococcal polysaccharide antigen	870:904	the enterococcal polysaccharide antigen (epa) locus	866:916	From the identified colonization genes, epaX encodes a glycosyltransferase located in a variable region of the enterococcal polysaccharide antigen (epa) locus.
25035517	5	47	theme	identified	768:777	arg1	genes					792:796	the identified colonization genes	764:796	the identified colonization genes	764:796	From the identified colonization genes, epaX encodes a glycosyltransferase located in a variable region of the enterococcal polysaccharide antigen (epa) locus.
25035517	0	48	theme	rhamnopolysaccharide	12:31	arg1	determinant					71:81	a key determinant	65:81	a key determinant of intestinal colonization	65:108	The surface rhamnopolysaccharide epa of Enterococcus faecalis is a key determinant of intestinal colonization.
25035517	0	48	theme	rhamnopolysaccharide	12:31	arg1	epa					33:35	The surface rhamnopolysaccharide epa	0:35	The surface rhamnopolysaccharide epa of Enterococcus faecalis	0:60	The surface rhamnopolysaccharide epa of Enterococcus faecalis is a key determinant of intestinal colonization.
25035517	6	49	theme	sugar	953:957	arg1	composition					959:969	sugar composition	953:969	sugar composition	953:969	We demonstrated that EpaX acts on sugar composition, promoting resistance to bile salts and cell wall integrity.
25035517	6	50	theme	wall	1016:1019	arg1	integrity					1021:1029	cell wall integrity	1011:1029	cell wall integrity	1011:1029	We demonstrated that EpaX acts on sugar composition, promoting resistance to bile salts and cell wall integrity.
27721544	7	0	theme	Sw	1144:1145	arg1	FrIV					1147:1150	Sw FrIV	1144:1150	Sw FrIV	1144:1150	Sw FrIV and Ho FrIV significantly inhibited paw edema induced by carrageenan, especially at 3 h after treatment and potentially decreased neutrophil migration at 10 mg/kg, respectively.
27721544	10	1	theme	fractions	1712:1720	arg1	effect					1693:1698	the effect	1689:1698	the effect of purified fractions on inhibition of nitric oxide synthase expression mediated by inhibiting the phosphorylation of various signal proteins in lipopolysaccharide-stimulated RAW264.7 cells	1689:1888	However, to determine the molecular mechanism, it is necessary to investigate the effect of purified fractions on inhibition of nitric oxide synthase expression mediated by inhibiting the phosphorylation of various signal proteins in lipopolysaccharide-stimulated RAW264.7 cells.
27721544	1	2	theme	fractions	280:288	arg1	potential					234:242	the antinociceptive and anti-inflammatory potential	192:242	the antinociceptive and anti-inflammatory potential of sulfated polysaccharide purified fractions isolated from brown seaweed Sargassum wightii and seagrass	192:347	AIM The aim of this objective is to evaluate the antinociceptive and anti-inflammatory potential of sulfated polysaccharide purified fractions isolated from brown seaweed Sargassum wightii and seagrass Halophila ovalis in male Wistar rats.
27721544	9	3	from	H.	1527:1528	arg1	components					1496:1505	CONCLUSION Purified components	1476:1505	CONCLUSION Purified components from S. wightii and H. ovalis	1476:1535	CONCLUSION Purified components from S. wightii and H. ovalis have strong antinociceptive and anti-inflammatory effect on animal model.
27721544	8	4	theme	significant	1377:1387	arg1	reduction					1389:1397	a significant reduction	1375:1397	a significant reduction in paw volume	1375:1411	In Freund's adjuvant-induced arthritic rats, a significant reduction in paw volume was observed in Sw FrIV and Ho FrIV-treated groups (10 mg/kg).
27721544	3	5	theme	purified	595:602	arg1	fractions					604:612	purified fractions	595:612	purified fractions	595:612	The antinociceptive activity of purified fractions was investigated using formalin and hot plate test.
27721544	9	6	from	wightii	1515:1521	arg1	components					1496:1505	CONCLUSION Purified components	1476:1505	CONCLUSION Purified components from S. wightii and H. ovalis	1476:1535	CONCLUSION Purified components from S. wightii and H. ovalis have strong antinociceptive and anti-inflammatory effect on animal model.
27721544	0	7	theme	Antinociceptive	0:14	arg1	effect					38:43	Antinociceptive and anti-inflammatory effect	0:43	Antinociceptive and anti-inflammatory effect of sulfated polysaccharide fractions from Sargassum wightii and Halophila ovalis in male Wistar rats.	0:145	Antinociceptive and anti-inflammatory effect of sulfated polysaccharide fractions from Sargassum wightii and Halophila ovalis in male Wistar rats.
27721544	5	8	theme	test	932:935	arg1	phases					918:923	both phases	913:923	both phases of the test	913:935	RESULTS In the formalin test, there was a significant reduction in licking time in both phases of the test at a dose of 10 mg/kg.
27721544	6	9	theme	antinociceptive	987:1001	arg1	effect					1003:1008	the antinociceptive effect	983:1008	the antinociceptive effect	983:1008	In the hot plate test, the antinociceptive effect was observed only in animals treated with 5, 10 mg/kg suggesting that the analgesic effect occurs through a central action mechanism.
27721544	0	10	theme	Wistar	134:139	arg1	rats					141:144	male Wistar rats	129:144	male Wistar rats	129:144	Antinociceptive and anti-inflammatory effect of sulfated polysaccharide fractions from Sargassum wightii and Halophila ovalis in male Wistar rats.
27721544	1	11	theme	antinociceptive	196:210	arg1	potential					234:242	the antinociceptive and anti-inflammatory potential	192:242	the antinociceptive and anti-inflammatory potential of sulfated polysaccharide purified fractions isolated from brown seaweed Sargassum wightii and seagrass	192:347	AIM The aim of this objective is to evaluate the antinociceptive and anti-inflammatory potential of sulfated polysaccharide purified fractions isolated from brown seaweed Sargassum wightii and seagrass Halophila ovalis in male Wistar rats.
27721544	9	12	theme	Purified	1487:1494	arg1	components					1496:1505	CONCLUSION Purified components	1476:1505	CONCLUSION Purified components from S. wightii and H. ovalis	1476:1535	CONCLUSION Purified components from S. wightii and H. ovalis have strong antinociceptive and anti-inflammatory effect on animal model.
27721544	5	13	dep	RESULTS	830:836	arg1	was					866:868	was	866:868	was a significant reduction in licking time in both phases of the test at a dose of 10 mg/kg	866:957	RESULTS In the formalin test, there was a significant reduction in licking time in both phases of the test at a dose of 10 mg/kg.
27721544	0	14	from	wightii	97:103	arg1	effect					38:43	Antinociceptive and anti-inflammatory effect	0:43	Antinociceptive and anti-inflammatory effect of sulfated polysaccharide fractions from Sargassum wightii and Halophila ovalis in male Wistar rats.	0:145	Antinociceptive and anti-inflammatory effect of sulfated polysaccharide fractions from Sargassum wightii and Halophila ovalis in male Wistar rats.
27721544	0	14	from	wightii	97:103	arg1	fractions					72:80	sulfated polysaccharide fractions	48:80	sulfated polysaccharide fractions from Sargassum wightii and Halophila ovalis	48:124	Antinociceptive and anti-inflammatory effect of sulfated polysaccharide fractions from Sargassum wightii and Halophila ovalis in male Wistar rats.
27721544	10	15	theme	molecular	1637:1645	arg1	mechanism					1647:1655	the molecular mechanism	1633:1655	the molecular mechanism	1633:1655	However, to determine the molecular mechanism, it is necessary to investigate the effect of purified fractions on inhibition of nitric oxide synthase expression mediated by inhibiting the phosphorylation of various signal proteins in lipopolysaccharide-stimulated RAW264.7 cells.
27721544	3	16	theme	plate	654:658	arg1	test					660:663	hot plate test	650:663	hot plate test	650:663	The antinociceptive activity of purified fractions was investigated using formalin and hot plate test.
27721544	1	17	theme	anti-inflammatory	216:232	arg1	potential					234:242	the antinociceptive and anti-inflammatory potential	192:242	the antinociceptive and anti-inflammatory potential of sulfated polysaccharide purified fractions isolated from brown seaweed Sargassum wightii and seagrass	192:347	AIM The aim of this objective is to evaluate the antinociceptive and anti-inflammatory potential of sulfated polysaccharide purified fractions isolated from brown seaweed Sargassum wightii and seagrass Halophila ovalis in male Wistar rats.
27721544	6	18	theme	plate	971:975	arg1	test					977:980	the hot plate test	963:980	the hot plate test	963:980	In the hot plate test, the antinociceptive effect was observed only in animals treated with 5, 10 mg/kg suggesting that the analgesic effect occurs through a central action mechanism.
27721544	8	19	dep	FrIV	1432:1435	arg1	groups					1457:1462	FrIV-treated groups	1444:1462	FrIV-treated groups (10 mg/kg)	1444:1473	In Freund's adjuvant-induced arthritic rats, a significant reduction in paw volume was observed in Sw FrIV and Ho FrIV-treated groups (10 mg/kg).
27721544	8	19	dep	FrIV	1432:1435	arg1	mg/kg					1468:1472	10 mg/kg	1465:1472	10 mg/kg	1465:1472	In Freund's adjuvant-induced arthritic rats, a significant reduction in paw volume was observed in Sw FrIV and Ho FrIV-treated groups (10 mg/kg).
27721544	10	20	theme	lipopolysaccharide-stimulated	1845:1873	arg1	cells					1884:1888	lipopolysaccharide-stimulated RAW264.7 cells	1845:1888	lipopolysaccharide-stimulated RAW264.7 cells	1845:1888	However, to determine the molecular mechanism, it is necessary to investigate the effect of purified fractions on inhibition of nitric oxide synthase expression mediated by inhibiting the phosphorylation of various signal proteins in lipopolysaccharide-stimulated RAW264.7 cells.
27721544	4	21	theme	Adjuvant-induced	738:753	arg1	model					765:769	Freund's Complete Adjuvant-induced arthritis model	720:769	Freund's Complete Adjuvant-induced arthritis model	720:769	Carrageenan-induced paw edema, peritonitis model, and Freund's Complete Adjuvant-induced arthritis model were employed to determine the anti-inflammatory activity.
27721544	1	22	attach	isolated	290:297	arg1	seagrass					340:347	seagrass	340:347	seagrass	340:347	AIM The aim of this objective is to evaluate the antinociceptive and anti-inflammatory potential of sulfated polysaccharide purified fractions isolated from brown seaweed Sargassum wightii and seagrass Halophila ovalis in male Wistar rats.
27721544	1	22	attach	isolated	290:297	arg2	fractions					280:288	sulfated polysaccharide purified fractions	247:288	sulfated polysaccharide purified fractions isolated from brown seaweed Sargassum wightii and seagrass	247:347	AIM The aim of this objective is to evaluate the antinociceptive and anti-inflammatory potential of sulfated polysaccharide purified fractions isolated from brown seaweed Sargassum wightii and seagrass Halophila ovalis in male Wistar rats.
27721544	1	22	attach	isolated	290:297	arg1	wightii					328:334	brown seaweed Sargassum wightii	304:334	brown seaweed Sargassum wightii	304:334	AIM The aim of this objective is to evaluate the antinociceptive and anti-inflammatory potential of sulfated polysaccharide purified fractions isolated from brown seaweed Sargassum wightii and seagrass Halophila ovalis in male Wistar rats.
27721544	5	23	theme	formalin	845:852	arg1	test					854:857	the formalin test	841:857	the formalin test	841:857	RESULTS In the formalin test, there was a significant reduction in licking time in both phases of the test at a dose of 10 mg/kg.
27721544	1	24	theme	Wistar	374:379	arg1	rats					381:384	male Wistar rats	369:384	male Wistar rats	369:384	AIM The aim of this objective is to evaluate the antinociceptive and anti-inflammatory potential of sulfated polysaccharide purified fractions isolated from brown seaweed Sargassum wightii and seagrass Halophila ovalis in male Wistar rats.
27721544	8	25	theme	arthritic	1359:1367	arg1	rats					1369:1372	Freund's adjuvant-induced arthritic rats	1333:1372	Freund's adjuvant-induced arthritic rats	1333:1372	In Freund's adjuvant-induced arthritic rats, a significant reduction in paw volume was observed in Sw FrIV and Ho FrIV-treated groups (10 mg/kg).
27721544	5	26	from	reduction	884:892	arg1	time					905:908	licking time	897:908	licking time	897:908	RESULTS In the formalin test, there was a significant reduction in licking time in both phases of the test at a dose of 10 mg/kg.
27721544	5	26	from	reduction	884:892	arg1	phases					918:923	both phases	913:923	both phases of the test	913:935	RESULTS In the formalin test, there was a significant reduction in licking time in both phases of the test at a dose of 10 mg/kg.
27721544	10	27	theme	synthase	1752:1759	arg1	expression					1761:1770	nitric oxide synthase expression	1739:1770	nitric oxide synthase expression mediated by inhibiting the phosphorylation of various signal proteins in lipopolysaccharide-stimulated RAW264.7 cells	1739:1888	However, to determine the molecular mechanism, it is necessary to investigate the effect of purified fractions on inhibition of nitric oxide synthase expression mediated by inhibiting the phosphorylation of various signal proteins in lipopolysaccharide-stimulated RAW264.7 cells.
27721544	0	28	theme	sulfated	48:55	arg1	fractions					72:80	sulfated polysaccharide fractions	48:80	sulfated polysaccharide fractions from Sargassum wightii and Halophila ovalis	48:124	Antinociceptive and anti-inflammatory effect of sulfated polysaccharide fractions from Sargassum wightii and Halophila ovalis in male Wistar rats.
27721544	9	29	theme	antinociceptive	1549:1563	arg1	effect					1587:1592	strong antinociceptive and anti-inflammatory effect	1542:1592	strong antinociceptive and anti-inflammatory effect	1542:1592	CONCLUSION Purified components from S. wightii and H. ovalis have strong antinociceptive and anti-inflammatory effect on animal model.
27721544	6	30	theme	analgesic	1084:1092	arg1	effect					1094:1099	the analgesic effect	1080:1099	the analgesic effect	1080:1099	In the hot plate test, the antinociceptive effect was observed only in animals treated with 5, 10 mg/kg suggesting that the analgesic effect occurs through a central action mechanism.
27721544	4	31	theme	anti-inflammatory	802:818	arg1	activity					820:827	the anti-inflammatory activity	798:827	the anti-inflammatory activity	798:827	Carrageenan-induced paw edema, peritonitis model, and Freund's Complete Adjuvant-induced arthritis model were employed to determine the anti-inflammatory activity.
27721544	2	32	theme	sulfated	414:421	arg1	polysaccharide					423:436	SUBJECTS AND METHODS Crude sulfated polysaccharide	387:436	SUBJECTS AND METHODS Crude sulfated polysaccharide from S. wightii and H. ovalis	387:466	SUBJECTS AND METHODS Crude sulfated polysaccharide from S. wightii and H. ovalis was subjected to anion exchange chromatography, and the chemical composition was investigated.
27721544	5	33	theme	significant	872:882	arg1	reduction					884:892	a significant reduction	870:892	a significant reduction in licking time in both phases of the test	870:935	RESULTS In the formalin test, there was a significant reduction in licking time in both phases of the test at a dose of 10 mg/kg.
27721544	1	34	theme	brown	304:308	arg1	wightii					328:334	brown seaweed Sargassum wightii	304:334	brown seaweed Sargassum wightii	304:334	AIM The aim of this objective is to evaluate the antinociceptive and anti-inflammatory potential of sulfated polysaccharide purified fractions isolated from brown seaweed Sargassum wightii and seagrass Halophila ovalis in male Wistar rats.
27721544	9	35	theme	anti-inflammatory	1569:1585	arg1	effect					1587:1592	strong antinociceptive and anti-inflammatory effect	1542:1592	strong antinociceptive and anti-inflammatory effect	1542:1592	CONCLUSION Purified components from S. wightii and H. ovalis have strong antinociceptive and anti-inflammatory effect on animal model.
27721544	0	36	theme	anti-inflammatory	20:36	arg1	effect					38:43	Antinociceptive and anti-inflammatory effect	0:43	Antinociceptive and anti-inflammatory effect of sulfated polysaccharide fractions from Sargassum wightii and Halophila ovalis in male Wistar rats.	0:145	Antinociceptive and anti-inflammatory effect of sulfated polysaccharide fractions from Sargassum wightii and Halophila ovalis in male Wistar rats.
27721544	1	37	theme	Sargassum	318:326	arg1	wightii					328:334	brown seaweed Sargassum wightii	304:334	brown seaweed Sargassum wightii	304:334	AIM The aim of this objective is to evaluate the antinociceptive and anti-inflammatory potential of sulfated polysaccharide purified fractions isolated from brown seaweed Sargassum wightii and seagrass Halophila ovalis in male Wistar rats.
27721544	10	38	theme	oxide	1746:1750	arg1	synthase					1752:1759	nitric oxide synthase	1739:1759	nitric oxide synthase expression mediated by inhibiting the phosphorylation of various signal proteins in lipopolysaccharide-stimulated RAW264.7 cells	1739:1888	However, to determine the molecular mechanism, it is necessary to investigate the effect of purified fractions on inhibition of nitric oxide synthase expression mediated by inhibiting the phosphorylation of various signal proteins in lipopolysaccharide-stimulated RAW264.7 cells.
27721544	2	39	theme	exchange	491:498	arg1	chromatography					500:513	anion exchange chromatography	485:513	anion exchange chromatography	485:513	SUBJECTS AND METHODS Crude sulfated polysaccharide from S. wightii and H. ovalis was subjected to anion exchange chromatography, and the chemical composition was investigated.
27721544	2	40	dep	H.	458:459	arg1	ovalis					461:466	H. ovalis	458:466	H. ovalis	458:466	SUBJECTS AND METHODS Crude sulfated polysaccharide from S. wightii and H. ovalis was subjected to anion exchange chromatography, and the chemical composition was investigated.
27721544	10	41	theme	purified	1703:1710	arg1	fractions					1712:1720	purified fractions	1703:1720	purified fractions	1703:1720	However, to determine the molecular mechanism, it is necessary to investigate the effect of purified fractions on inhibition of nitric oxide synthase expression mediated by inhibiting the phosphorylation of various signal proteins in lipopolysaccharide-stimulated RAW264.7 cells.
27721544	0	42	theme	polysaccharide	57:70	arg1	fractions					72:80	sulfated polysaccharide fractions	48:80	sulfated polysaccharide fractions from Sargassum wightii and Halophila ovalis	48:124	Antinociceptive and anti-inflammatory effect of sulfated polysaccharide fractions from Sargassum wightii and Halophila ovalis in male Wistar rats.
27721544	10	43	theme	proteins	1833:1840	arg1	phosphorylation					1799:1813	the phosphorylation	1795:1813	the phosphorylation of various signal proteins in lipopolysaccharide-stimulated RAW264.7 cells	1795:1888	However, to determine the molecular mechanism, it is necessary to investigate the effect of purified fractions on inhibition of nitric oxide synthase expression mediated by inhibiting the phosphorylation of various signal proteins in lipopolysaccharide-stimulated RAW264.7 cells.
27721544	10	44	theme	nitric	1739:1744	arg1	synthase					1752:1759	nitric oxide synthase	1739:1759	nitric oxide synthase expression mediated by inhibiting the phosphorylation of various signal proteins in lipopolysaccharide-stimulated RAW264.7 cells	1739:1888	However, to determine the molecular mechanism, it is necessary to investigate the effect of purified fractions on inhibition of nitric oxide synthase expression mediated by inhibiting the phosphorylation of various signal proteins in lipopolysaccharide-stimulated RAW264.7 cells.
27721544	2	45	from	wightii	446:452	arg1	polysaccharide					423:436	SUBJECTS AND METHODS Crude sulfated polysaccharide	387:436	SUBJECTS AND METHODS Crude sulfated polysaccharide from S. wightii and H. ovalis	387:466	SUBJECTS AND METHODS Crude sulfated polysaccharide from S. wightii and H. ovalis was subjected to anion exchange chromatography, and the chemical composition was investigated.
27721544	7	46	theme	neutrophil	1282:1291	arg1	migration					1293:1301	neutrophil migration	1282:1301	neutrophil migration	1282:1301	Sw FrIV and Ho FrIV significantly inhibited paw edema induced by carrageenan, especially at 3 h after treatment and potentially decreased neutrophil migration at 10 mg/kg, respectively.
27721544	2	47	theme	METHODS	400:406	arg1	polysaccharide					423:436	SUBJECTS AND METHODS Crude sulfated polysaccharide	387:436	SUBJECTS AND METHODS Crude sulfated polysaccharide from S. wightii and H. ovalis	387:466	SUBJECTS AND METHODS Crude sulfated polysaccharide from S. wightii and H. ovalis was subjected to anion exchange chromatography, and the chemical composition was investigated.
27721544	1	48	theme	sulfated	247:254	arg1	fractions					280:288	sulfated polysaccharide purified fractions	247:288	sulfated polysaccharide purified fractions isolated from brown seaweed Sargassum wightii and seagrass	247:347	AIM The aim of this objective is to evaluate the antinociceptive and anti-inflammatory potential of sulfated polysaccharide purified fractions isolated from brown seaweed Sargassum wightii and seagrass Halophila ovalis in male Wistar rats.
27721544	2	49	from	H.	458:459	arg1	polysaccharide					423:436	SUBJECTS AND METHODS Crude sulfated polysaccharide	387:436	SUBJECTS AND METHODS Crude sulfated polysaccharide from S. wightii and H. ovalis	387:466	SUBJECTS AND METHODS Crude sulfated polysaccharide from S. wightii and H. ovalis was subjected to anion exchange chromatography, and the chemical composition was investigated.
27721544	4	50	theme	Carrageenan-induced	666:684	arg1	edema					690:694	Carrageenan-induced paw edema	666:694	Carrageenan-induced paw edema	666:694	Carrageenan-induced paw edema, peritonitis model, and Freund's Complete Adjuvant-induced arthritis model were employed to determine the anti-inflammatory activity.
27721544	9	51	contain	have	1537:1540	arg1	components					1496:1505	CONCLUSION Purified components	1476:1505	CONCLUSION Purified components from S. wightii and H. ovalis	1476:1535	CONCLUSION Purified components from S. wightii and H. ovalis have strong antinociceptive and anti-inflammatory effect on animal model.
27721544	9	51	contain	have	1537:1540	arg2	effect					1587:1592	strong antinociceptive and anti-inflammatory effect	1542:1592	strong antinociceptive and anti-inflammatory effect	1542:1592	CONCLUSION Purified components from S. wightii and H. ovalis have strong antinociceptive and anti-inflammatory effect on animal model.
27721544	2	52	theme	SUBJECTS	387:394	arg1	polysaccharide					423:436	SUBJECTS AND METHODS Crude sulfated polysaccharide	387:436	SUBJECTS AND METHODS Crude sulfated polysaccharide from S. wightii and H. ovalis	387:466	SUBJECTS AND METHODS Crude sulfated polysaccharide from S. wightii and H. ovalis was subjected to anion exchange chromatography, and the chemical composition was investigated.
27721544	8	53	from	reduction	1389:1397	arg1	volume					1406:1411	paw volume	1402:1411	paw volume	1402:1411	In Freund's adjuvant-induced arthritic rats, a significant reduction in paw volume was observed in Sw FrIV and Ho FrIV-treated groups (10 mg/kg).
27721544	1	54	theme	purified	271:278	arg1	fractions					280:288	sulfated polysaccharide purified fractions	247:288	sulfated polysaccharide purified fractions isolated from brown seaweed Sargassum wightii and seagrass	247:347	AIM The aim of this objective is to evaluate the antinociceptive and anti-inflammatory potential of sulfated polysaccharide purified fractions isolated from brown seaweed Sargassum wightii and seagrass Halophila ovalis in male Wistar rats.
27721544	5	55	theme	mg/kg	953:957	arg1	dose					942:945	a dose	940:945	a dose of 10 mg/kg	940:957	RESULTS In the formalin test, there was a significant reduction in licking time in both phases of the test at a dose of 10 mg/kg.
27721544	10	56	from	effect	1693:1698	arg1	inhibition					1725:1734	inhibition	1725:1734	inhibition of nitric oxide synthase expression mediated by inhibiting the phosphorylation of various signal proteins in lipopolysaccharide-stimulated RAW264.7 cells	1725:1888	However, to determine the molecular mechanism, it is necessary to investigate the effect of purified fractions on inhibition of nitric oxide synthase expression mediated by inhibiting the phosphorylation of various signal proteins in lipopolysaccharide-stimulated RAW264.7 cells.
27721544	10	57	theme	expression	1761:1770	arg1	inhibition					1725:1734	inhibition	1725:1734	inhibition of nitric oxide synthase expression mediated by inhibiting the phosphorylation of various signal proteins in lipopolysaccharide-stimulated RAW264.7 cells	1725:1888	However, to determine the molecular mechanism, it is necessary to investigate the effect of purified fractions on inhibition of nitric oxide synthase expression mediated by inhibiting the phosphorylation of various signal proteins in lipopolysaccharide-stimulated RAW264.7 cells.
27721544	3	58	theme	antinociceptive	567:581	arg1	activity					583:590	The antinociceptive activity	563:590	The antinociceptive activity of purified fractions	563:612	The antinociceptive activity of purified fractions was investigated using formalin and hot plate test.
27721544	1	59	theme	objective	167:175	arg1	aim					155:157	The aim	151:157	The aim of this objective	151:175	AIM The aim of this objective is to evaluate the antinociceptive and anti-inflammatory potential of sulfated polysaccharide purified fractions isolated from brown seaweed Sargassum wightii and seagrass Halophila ovalis in male Wistar rats.
27721544	3	60	theme	fractions	604:612	arg1	activity					583:590	The antinociceptive activity	563:590	The antinociceptive activity of purified fractions	563:612	The antinociceptive activity of purified fractions was investigated using formalin and hot plate test.
27721544	5	61	theme	licking	897:903	arg1	time					905:908	licking time	897:908	licking time	897:908	RESULTS In the formalin test, there was a significant reduction in licking time in both phases of the test at a dose of 10 mg/kg.
27721544	8	62	located	observed	1417:1424	arg1	rats					1369:1372	Freund's adjuvant-induced arthritic rats	1333:1372	Freund's adjuvant-induced arthritic rats	1333:1372	In Freund's adjuvant-induced arthritic rats, a significant reduction in paw volume was observed in Sw FrIV and Ho FrIV-treated groups (10 mg/kg).
27721544	8	62	located	observed	1417:1424	arg1	Ho					1441:1442	Ho	1441:1442	Ho	1441:1442	In Freund's adjuvant-induced arthritic rats, a significant reduction in paw volume was observed in Sw FrIV and Ho FrIV-treated groups (10 mg/kg).
27721544	8	62	located	observed	1417:1424	arg2	reduction					1389:1397	a significant reduction	1375:1397	a significant reduction in paw volume	1375:1411	In Freund's adjuvant-induced arthritic rats, a significant reduction in paw volume was observed in Sw FrIV and Ho FrIV-treated groups (10 mg/kg).
27721544	8	62	located	observed	1417:1424	arg1	FrIV					1432:1435	FrIV	1432:1435	FrIV	1432:1435	In Freund's adjuvant-induced arthritic rats, a significant reduction in paw volume was observed in Sw FrIV and Ho FrIV-treated groups (10 mg/kg).
27721544	8	63	theme	FrIV-treated	1444:1455	arg1	groups					1457:1462	FrIV-treated groups	1444:1462	FrIV-treated groups (10 mg/kg)	1444:1473	In Freund's adjuvant-induced arthritic rats, a significant reduction in paw volume was observed in Sw FrIV and Ho FrIV-treated groups (10 mg/kg).
27721544	8	63	theme	FrIV-treated	1444:1455	arg1	mg/kg					1468:1472	10 mg/kg	1465:1472	10 mg/kg	1465:1472	In Freund's adjuvant-induced arthritic rats, a significant reduction in paw volume was observed in Sw FrIV and Ho FrIV-treated groups (10 mg/kg).
27721544	3	64	theme	hot	650:652	arg1	test					660:663	hot plate test	650:663	hot plate test	650:663	The antinociceptive activity of purified fractions was investigated using formalin and hot plate test.
27721544	4	65	theme	peritonitis	697:707	arg1	model					709:713	peritonitis model	697:713	peritonitis model	697:713	Carrageenan-induced paw edema, peritonitis model, and Freund's Complete Adjuvant-induced arthritis model were employed to determine the anti-inflammatory activity.
27721544	6	66	theme	hot	967:969	arg1	test					977:980	the hot plate test	963:980	the hot plate test	963:980	In the hot plate test, the antinociceptive effect was observed only in animals treated with 5, 10 mg/kg suggesting that the analgesic effect occurs through a central action mechanism.
27721544	10	67	theme	RAW264.7	1875:1882	arg1	cells					1884:1888	lipopolysaccharide-stimulated RAW264.7 cells	1845:1888	lipopolysaccharide-stimulated RAW264.7 cells	1845:1888	However, to determine the molecular mechanism, it is necessary to investigate the effect of purified fractions on inhibition of nitric oxide synthase expression mediated by inhibiting the phosphorylation of various signal proteins in lipopolysaccharide-stimulated RAW264.7 cells.
27721544	0	68	from	ovalis	119:124	arg1	effect					38:43	Antinociceptive and anti-inflammatory effect	0:43	Antinociceptive and anti-inflammatory effect of sulfated polysaccharide fractions from Sargassum wightii and Halophila ovalis in male Wistar rats.	0:145	Antinociceptive and anti-inflammatory effect of sulfated polysaccharide fractions from Sargassum wightii and Halophila ovalis in male Wistar rats.
27721544	0	68	from	ovalis	119:124	arg1	fractions					72:80	sulfated polysaccharide fractions	48:80	sulfated polysaccharide fractions from Sargassum wightii and Halophila ovalis	48:124	Antinociceptive and anti-inflammatory effect of sulfated polysaccharide fractions from Sargassum wightii and Halophila ovalis in male Wistar rats.
27721544	2	69	theme	chemical	524:531	arg1	composition					533:543	the chemical composition	520:543	the chemical composition	520:543	SUBJECTS AND METHODS Crude sulfated polysaccharide from S. wightii and H. ovalis was subjected to anion exchange chromatography, and the chemical composition was investigated.
27721544	0	70	from	effect	38:43	arg1	wightii					97:103	Sargassum wightii	87:103	Sargassum wightii	87:103	Antinociceptive and anti-inflammatory effect of sulfated polysaccharide fractions from Sargassum wightii and Halophila ovalis in male Wistar rats.
27721544	0	70	from	effect	38:43	arg1	ovalis					119:124	Halophila ovalis	109:124	Halophila ovalis	109:124	Antinociceptive and anti-inflammatory effect of sulfated polysaccharide fractions from Sargassum wightii and Halophila ovalis in male Wistar rats.
27721544	0	70	from	effect	38:43	arg1	rats					141:144	male Wistar rats	129:144	male Wistar rats	129:144	Antinociceptive and anti-inflammatory effect of sulfated polysaccharide fractions from Sargassum wightii and Halophila ovalis in male Wistar rats.
27721544	4	71	theme	Complete	729:736	arg1	model					765:769	Freund's Complete Adjuvant-induced arthritis model	720:769	Freund's Complete Adjuvant-induced arthritis model	720:769	Carrageenan-induced paw edema, peritonitis model, and Freund's Complete Adjuvant-induced arthritis model were employed to determine the anti-inflammatory activity.
27721544	1	72	theme	male	369:372	arg1	rats					381:384	male Wistar rats	369:384	male Wistar rats	369:384	AIM The aim of this objective is to evaluate the antinociceptive and anti-inflammatory potential of sulfated polysaccharide purified fractions isolated from brown seaweed Sargassum wightii and seagrass Halophila ovalis in male Wistar rats.
27721544	4	73	theme	arthritis	755:763	arg1	model					765:769	Freund's Complete Adjuvant-induced arthritis model	720:769	Freund's Complete Adjuvant-induced arthritis model	720:769	Carrageenan-induced paw edema, peritonitis model, and Freund's Complete Adjuvant-induced arthritis model were employed to determine the anti-inflammatory activity.
27721544	8	74	theme	adjuvant-induced	1342:1357	arg1	rats					1369:1372	Freund's adjuvant-induced arthritic rats	1333:1372	Freund's adjuvant-induced arthritic rats	1333:1372	In Freund's adjuvant-induced arthritic rats, a significant reduction in paw volume was observed in Sw FrIV and Ho FrIV-treated groups (10 mg/kg).
27721544	1	75	dep	AIM	147:149	arg1	is					177:178	is	177:178	is to evaluate the antinociceptive and anti-inflammatory potential of sulfated polysaccharide purified fractions isolated from brown seaweed Sargassum wightii and seagrass Halophila ovalis in male Wistar rats	177:384	AIM The aim of this objective is to evaluate the antinociceptive and anti-inflammatory potential of sulfated polysaccharide purified fractions isolated from brown seaweed Sargassum wightii and seagrass Halophila ovalis in male Wistar rats.
27721544	6	76	theme	action	1126:1131	arg1	mechanism					1133:1141	a central action mechanism	1116:1141	a central action mechanism	1116:1141	In the hot plate test, the antinociceptive effect was observed only in animals treated with 5, 10 mg/kg suggesting that the analgesic effect occurs through a central action mechanism.
27721544	0	77	theme	male	129:132	arg1	rats					141:144	male Wistar rats	129:144	male Wistar rats	129:144	Antinociceptive and anti-inflammatory effect of sulfated polysaccharide fractions from Sargassum wightii and Halophila ovalis in male Wistar rats.
27721544	9	78	theme	strong	1542:1547	arg1	effect					1587:1592	strong antinociceptive and anti-inflammatory effect	1542:1592	strong antinociceptive and anti-inflammatory effect	1542:1592	CONCLUSION Purified components from S. wightii and H. ovalis have strong antinociceptive and anti-inflammatory effect on animal model.
27721544	10	79	from	phosphorylation	1799:1813	arg1	cells					1884:1888	lipopolysaccharide-stimulated RAW264.7 cells	1845:1888	lipopolysaccharide-stimulated RAW264.7 cells	1845:1888	However, to determine the molecular mechanism, it is necessary to investigate the effect of purified fractions on inhibition of nitric oxide synthase expression mediated by inhibiting the phosphorylation of various signal proteins in lipopolysaccharide-stimulated RAW264.7 cells.
27721544	6	80	located	observed	1014:1021	arg2	effect					1003:1008	the antinociceptive effect	983:1008	the antinociceptive effect	983:1008	In the hot plate test, the antinociceptive effect was observed only in animals treated with 5, 10 mg/kg suggesting that the analgesic effect occurs through a central action mechanism.
27721544	6	80	located	observed	1014:1021	arg1	test					977:980	the hot plate test	963:980	the hot plate test	963:980	In the hot plate test, the antinociceptive effect was observed only in animals treated with 5, 10 mg/kg suggesting that the analgesic effect occurs through a central action mechanism.
27721544	6	80	located	observed	1014:1021	arg1	animals					1031:1037	animals	1031:1037	animals treated with 5, 10 mg/kg	1031:1062	In the hot plate test, the antinociceptive effect was observed only in animals treated with 5, 10 mg/kg suggesting that the analgesic effect occurs through a central action mechanism.
27721544	7	81	theme	paw	1188:1190	arg1	edema					1192:1196	paw edema	1188:1196	paw edema induced by carrageenan	1188:1219	Sw FrIV and Ho FrIV significantly inhibited paw edema induced by carrageenan, especially at 3 h after treatment and potentially decreased neutrophil migration at 10 mg/kg, respectively.
27721544	9	82	dep	H.	1527:1528	arg1	ovalis					1530:1535	H. ovalis	1527:1535	H. ovalis	1527:1535	CONCLUSION Purified components from S. wightii and H. ovalis have strong antinociceptive and anti-inflammatory effect on animal model.
27721544	2	83	theme	Crude	408:412	arg1	polysaccharide					423:436	SUBJECTS AND METHODS Crude sulfated polysaccharide	387:436	SUBJECTS AND METHODS Crude sulfated polysaccharide from S. wightii and H. ovalis	387:466	SUBJECTS AND METHODS Crude sulfated polysaccharide from S. wightii and H. ovalis was subjected to anion exchange chromatography, and the chemical composition was investigated.
27721544	1	84	theme	seaweed	310:316	arg1	wightii					328:334	brown seaweed Sargassum wightii	304:334	brown seaweed Sargassum wightii	304:334	AIM The aim of this objective is to evaluate the antinociceptive and anti-inflammatory potential of sulfated polysaccharide purified fractions isolated from brown seaweed Sargassum wightii and seagrass Halophila ovalis in male Wistar rats.
27721544	10	85	theme	signal	1826:1831	arg1	proteins					1833:1840	various signal proteins	1818:1840	various signal proteins	1818:1840	However, to determine the molecular mechanism, it is necessary to investigate the effect of purified fractions on inhibition of nitric oxide synthase expression mediated by inhibiting the phosphorylation of various signal proteins in lipopolysaccharide-stimulated RAW264.7 cells.
27721544	7	86	theme	Ho	1156:1157	arg1	FrIV					1159:1162	Ho FrIV	1156:1162	Ho FrIV	1156:1162	Sw FrIV and Ho FrIV significantly inhibited paw edema induced by carrageenan, especially at 3 h after treatment and potentially decreased neutrophil migration at 10 mg/kg, respectively.
27721544	9	87	theme	animal	1597:1602	arg1	model					1604:1608	animal model	1597:1608	animal model	1597:1608	CONCLUSION Purified components from S. wightii and H. ovalis have strong antinociceptive and anti-inflammatory effect on animal model.
27721544	8	88	theme	paw	1402:1404	arg1	volume					1406:1411	paw volume	1402:1411	paw volume	1402:1411	In Freund's adjuvant-induced arthritic rats, a significant reduction in paw volume was observed in Sw FrIV and Ho FrIV-treated groups (10 mg/kg).
27721544	0	89	theme	fractions	72:80	arg1	effect					38:43	Antinociceptive and anti-inflammatory effect	0:43	Antinociceptive and anti-inflammatory effect of sulfated polysaccharide fractions from Sargassum wightii and Halophila ovalis in male Wistar rats.	0:145	Antinociceptive and anti-inflammatory effect of sulfated polysaccharide fractions from Sargassum wightii and Halophila ovalis in male Wistar rats.
27721544	2	90	theme	anion	485:489	arg1	chromatography					500:513	anion exchange chromatography	485:513	anion exchange chromatography	485:513	SUBJECTS AND METHODS Crude sulfated polysaccharide from S. wightii and H. ovalis was subjected to anion exchange chromatography, and the chemical composition was investigated.
27721544	10	91	theme	various	1818:1824	arg1	proteins					1833:1840	various signal proteins	1818:1840	various signal proteins	1818:1840	However, to determine the molecular mechanism, it is necessary to investigate the effect of purified fractions on inhibition of nitric oxide synthase expression mediated by inhibiting the phosphorylation of various signal proteins in lipopolysaccharide-stimulated RAW264.7 cells.
27721544	9	92	dep	Purified	1487:1494	arg1	CONCLUSION					1476:1485	CONCLUSION	1476:1485	CONCLUSION	1476:1485	CONCLUSION Purified components from S. wightii and H. ovalis have strong antinociceptive and anti-inflammatory effect on animal model.
27721544	6	93	theme	central	1118:1124	arg1	mechanism					1133:1141	a central action mechanism	1116:1141	a central action mechanism	1116:1141	In the hot plate test, the antinociceptive effect was observed only in animals treated with 5, 10 mg/kg suggesting that the analgesic effect occurs through a central action mechanism.
27721544	4	94	theme	paw	686:688	arg1	edema					690:694	Carrageenan-induced paw edema	666:694	Carrageenan-induced paw edema	666:694	Carrageenan-induced paw edema, peritonitis model, and Freund's Complete Adjuvant-induced arthritis model were employed to determine the anti-inflammatory activity.
27721544	1	95	theme	polysaccharide	256:269	arg1	fractions					280:288	sulfated polysaccharide purified fractions	247:288	sulfated polysaccharide purified fractions isolated from brown seaweed Sargassum wightii and seagrass	247:347	AIM The aim of this objective is to evaluate the antinociceptive and anti-inflammatory potential of sulfated polysaccharide purified fractions isolated from brown seaweed Sargassum wightii and seagrass Halophila ovalis in male Wistar rats.
27842846	6	0	theme	SEM	534:536	arg1	images					538:543	SEM images	534:543	SEM images	534:543	SEM images suggested that incorporating CN amounts higher than 3wt.
27842846	5	1	theme	weak	419:422	arg1	interaction					424:434	a weak interaction	417:434	a weak interaction between the polymer matrix and nanofiller	417:476	FTIR revealed the presence of a weak interaction between the polymer matrix and nanofiller, confirmed by a slightly increase in thermal stability.
27842846	9	2	theme	remnant	925:931	arg1	acid					940:943	remnant acetic acid	925:943	remnant acetic acid	925:943	Improved performance against water was obtained in composite materials comparing to the pure polymer matrix, as well as CH and CH-CN films did not show antibacterial activity demonstrating that remnant acetic acid, when no neutralization step is done, plays an important role in this property.
27842846	7	3	theme	phase-segregated	613:628	arg1	systems					630:636	phase-segregated systems	613:636	phase-segregated systems	613:636	% generates phase-segregated systems, and SAXS showed that CH avoid the typical organization of CN at concentrations below 5wt.
27842846	9	4	theme	pure	819:822	arg1	matrix					832:837	the pure polymer matrix	815:837	the pure polymer matrix	815:837	Improved performance against water was obtained in composite materials comparing to the pure polymer matrix, as well as CH and CH-CN films did not show antibacterial activity demonstrating that remnant acetic acid, when no neutralization step is done, plays an important role in this property.
27842846	9	5	theme	acetic	933:938	arg1	acid					940:943	remnant acetic acid	925:943	remnant acetic acid	925:943	Improved performance against water was obtained in composite materials comparing to the pure polymer matrix, as well as CH and CH-CN films did not show antibacterial activity demonstrating that remnant acetic acid, when no neutralization step is done, plays an important role in this property.
27842846	9	6	theme	polymer	824:830	arg1	matrix					832:837	the pure polymer matrix	815:837	the pure polymer matrix	815:837	Improved performance against water was obtained in composite materials comparing to the pure polymer matrix, as well as CH and CH-CN films did not show antibacterial activity demonstrating that remnant acetic acid, when no neutralization step is done, plays an important role in this property.
27842846	1	7	theme	Chitosan/cellulose	93:110	arg1	films					133:137	Chitosan/cellulose nanocrystals (CH-CN) films	93:137	Chitosan/cellulose nanocrystals (CH-CN) films	93:137	Chitosan/cellulose nanocrystals (CH-CN) films were obtained by casting of dispersions, and treated with NaOH for neutralization proposes.
27842846	4	8	theme	different	357:365	arg1	properties					367:376	the different properties	353:376	the different properties studied	353:384	Changes in the morphology of the systems were correlated with the different properties studied.
27842846	1	9	theme	nanocrystals	112:123	arg1	films					133:137	Chitosan/cellulose nanocrystals (CH-CN) films	93:137	Chitosan/cellulose nanocrystals (CH-CN) films	93:137	Chitosan/cellulose nanocrystals (CH-CN) films were obtained by casting of dispersions, and treated with NaOH for neutralization proposes.
27842846	4	10	from	Changes	291:297	arg1	morphology					306:315	the morphology	302:315	the morphology of the systems	302:330	Changes in the morphology of the systems were correlated with the different properties studied.
27842846	5	11	theme	slightly	494:501	arg1	increase					503:510	a slightly increase	492:510	a slightly increase in thermal stability	492:531	FTIR revealed the presence of a weak interaction between the polymer matrix and nanofiller, confirmed by a slightly increase in thermal stability.
27842846	5	12	from	increase	503:510	arg1	stability					523:531	thermal stability	515:531	thermal stability	515:531	FTIR revealed the presence of a weak interaction between the polymer matrix and nanofiller, confirmed by a slightly increase in thermal stability.
27842846	1	13	theme	CH-CN	126:130	arg1	films					133:137	Chitosan/cellulose nanocrystals (CH-CN) films	93:137	Chitosan/cellulose nanocrystals (CH-CN) films	93:137	Chitosan/cellulose nanocrystals (CH-CN) films were obtained by casting of dispersions, and treated with NaOH for neutralization proposes.
27842846	9	14	theme	composite	782:790	arg1	materials					792:800	composite materials	782:800	composite materials comparing to the pure polymer matrix, as well as CH	782:852	Improved performance against water was obtained in composite materials comparing to the pure polymer matrix, as well as CH and CH-CN films did not show antibacterial activity demonstrating that remnant acetic acid, when no neutralization step is done, plays an important role in this property.
27842846	6	15	theme	higher	585:590	arg1	amounts					577:583	CN amounts	574:583	CN amounts higher than 3wt	574:599	SEM images suggested that incorporating CN amounts higher than 3wt.
27842846	9	16	theme	Improved	731:738	arg1	performance					740:750	Improved performance	731:750	Improved performance against water	731:764	Improved performance against water was obtained in composite materials comparing to the pure polymer matrix, as well as CH and CH-CN films did not show antibacterial activity demonstrating that remnant acetic acid, when no neutralization step is done, plays an important role in this property.
27842846	0	17	theme	chitosan-cellulose	41:58	arg1	nanocrystals					60:71	neutralized chitosan-cellulose nanocrystals	29:71	neutralized chitosan-cellulose nanocrystals	29:71	Morphology and properties of neutralized chitosan-cellulose nanocrystals biocomposite films.
27842846	7	18	theme	CN	697:698	arg1	organization					681:692	the typical organization	669:692	the typical organization of CN	669:698	% generates phase-segregated systems, and SAXS showed that CH avoid the typical organization of CN at concentrations below 5wt.
27842846	0	19	theme	neutralized	29:39	arg1	nanocrystals					60:71	neutralized chitosan-cellulose nanocrystals	29:71	neutralized chitosan-cellulose nanocrystals	29:71	Morphology and properties of neutralized chitosan-cellulose nanocrystals biocomposite films.
27842846	6	20	theme	CN	574:575	arg1	amounts					577:583	CN amounts	574:583	CN amounts higher than 3wt	574:599	SEM images suggested that incorporating CN amounts higher than 3wt.
27842846	7	21	theme	typical	673:679	arg1	organization					681:692	the typical organization	669:692	the typical organization of CN	669:698	% generates phase-segregated systems, and SAXS showed that CH avoid the typical organization of CN at concentrations below 5wt.
27842846	2	22	theme	films	250:254	arg1	composition					235:245	The composition	231:245	The composition of films	231:254	The composition of films was varied from 1 to 10wt.
27842846	9	23	theme	CH-CN	858:862	arg1	films					864:868	CH-CN films	858:868	CH-CN films	858:868	Improved performance against water was obtained in composite materials comparing to the pure polymer matrix, as well as CH and CH-CN films did not show antibacterial activity demonstrating that remnant acetic acid, when no neutralization step is done, plays an important role in this property.
27842846	4	24	theme	systems	324:330	arg1	morphology					306:315	the morphology	302:315	the morphology of the systems	302:330	Changes in the morphology of the systems were correlated with the different properties studied.
27842846	0	25	theme	nanocrystals	60:71	arg1	properties					15:24	properties	15:24	properties	15:24	Morphology and properties of neutralized chitosan-cellulose nanocrystals biocomposite films.
27842846	0	25	theme	nanocrystals	60:71	arg1	Morphology					0:9	Morphology	0:9	Morphology	0:9	Morphology and properties of neutralized chitosan-cellulose nanocrystals biocomposite films.
27842846	5	26	theme	interaction	424:434	arg1	presence					405:412	the presence	401:412	the presence	401:412	FTIR revealed the presence of a weak interaction between the polymer matrix and nanofiller, confirmed by a slightly increase in thermal stability.
27842846	3	27	theme	CN	287:288	arg1	%					282:282	%	282:282	% of CN.	282:289	% of CN.
27842846	3	27	theme	CN	287:288	arg1	CN					287:288	CN	287:288	CN	287:288	% of CN.
27842846	9	28	theme	important	992:1000	arg1	role					1002:1005	an important role	989:1005	an important role	989:1005	Improved performance against water was obtained in composite materials comparing to the pure polymer matrix, as well as CH and CH-CN films did not show antibacterial activity demonstrating that remnant acetic acid, when no neutralization step is done, plays an important role in this property.
27842846	1	29	dep	neutralization	206:219	arg1	proposes					221:228	proposes	221:228	proposes	221:228	Chitosan/cellulose nanocrystals (CH-CN) films were obtained by casting of dispersions, and treated with NaOH for neutralization proposes.
27842846	5	30	theme	polymer	448:454	arg1	matrix					456:461	the polymer matrix	444:461	the polymer matrix	444:461	FTIR revealed the presence of a weak interaction between the polymer matrix and nanofiller, confirmed by a slightly increase in thermal stability.
27842846	9	31	theme	antibacterial	883:895	arg1	activity					897:904	antibacterial activity	883:904	antibacterial activity demonstrating that remnant acetic acid, when no neutralization step is done, plays an important role in this property	883:1022	Improved performance against water was obtained in composite materials comparing to the pure polymer matrix, as well as CH and CH-CN films did not show antibacterial activity demonstrating that remnant acetic acid, when no neutralization step is done, plays an important role in this property.
27842846	9	32	theme	neutralization	954:967	arg1	step					969:972	no neutralization step	951:972	no neutralization step	951:972	Improved performance against water was obtained in composite materials comparing to the pure polymer matrix, as well as CH and CH-CN films did not show antibacterial activity demonstrating that remnant acetic acid, when no neutralization step is done, plays an important role in this property.
27842846	5	33	theme	thermal	515:521	arg1	stability					523:531	thermal stability	515:531	thermal stability	515:531	FTIR revealed the presence of a weak interaction between the polymer matrix and nanofiller, confirmed by a slightly increase in thermal stability.
27842846	1	34	theme	dispersions	167:177	arg1	casting					156:162	casting	156:162	casting of dispersions	156:177	Chitosan/cellulose nanocrystals (CH-CN) films were obtained by casting of dispersions, and treated with NaOH for neutralization proposes.
27719902	3	0	theme	0.6	682:684	arg1	absorption					846:855	the absorption	842:855	the absorption of water by the starch granules	842:887	Moreover, the protein-starch synergistic effect on the storage modulus was strongest at fractions of 0.5 and 0.6, due to the formation of a semi-interpenetrating network, with more amylose from the melted starch granules interpenetrated with the protein molecules, and the absorption of water by the starch granules to concentrate the protein matrix.
27719902	3	0	theme	0.6	682:684	arg1	fractions					661:669	fractions	661:669	fractions of 0.5 and 0.6, due to the formation of a semi-interpenetrating network, with more amylose from the melted starch granules interpenetrated with the protein molecules,	661:836	Moreover, the protein-starch synergistic effect on the storage modulus was strongest at fractions of 0.5 and 0.6, due to the formation of a semi-interpenetrating network, with more amylose from the melted starch granules interpenetrated with the protein molecules, and the absorption of water by the starch granules to concentrate the protein matrix.
27719902	1	1	theme	fraction	176:183	arg1	range					185:189	the starch fraction range	165:189	the starch fraction range from 0 to 1	165:201	The changes in fish myofibrillar protein/cassava starch composites in the starch fraction range from 0 to 1, with their total content maintained at 60mg/mL, were investigated in terms of textural properties, rheological behaviours, morphology, spatial distribution and protein molecular structure.
27719902	4	2	theme	starch	1018:1023	arg1	fraction					1025:1032	starch fraction	1018:1032	starch fraction	1018:1032	Additionally, no covalent interaction between the protein and starch occurred with increasing starch fraction, thus having no significant influence on the protein secondary structure.
27719902	4	3	theme	covalent	941:948	arg1	interaction					950:960	no covalent interaction	938:960	no covalent interaction between the protein and starch	938:991	Additionally, no covalent interaction between the protein and starch occurred with increasing starch fraction, thus having no significant influence on the protein secondary structure.
27719902	1	4	theme	spatial	339:345	arg1	distribution					347:358	spatial distribution	339:358	spatial distribution	339:358	The changes in fish myofibrillar protein/cassava starch composites in the starch fraction range from 0 to 1, with their total content maintained at 60mg/mL, were investigated in terms of textural properties, rheological behaviours, morphology, spatial distribution and protein molecular structure.
27719902	4	5	dep	protein	974:980	arg1	the					970:972	the	970:972	the	970:972	Additionally, no covalent interaction between the protein and starch occurred with increasing starch fraction, thus having no significant influence on the protein secondary structure.
27719902	1	6	theme	distribution	347:358	arg1	terms					273:277	terms	273:277	terms of textural properties, rheological behaviours, morphology, spatial distribution and protein molecular structure	273:390	The changes in fish myofibrillar protein/cassava starch composites in the starch fraction range from 0 to 1, with their total content maintained at 60mg/mL, were investigated in terms of textural properties, rheological behaviours, morphology, spatial distribution and protein molecular structure.
27719902	3	7	theme	starch	778:783	arg1	granules					785:792	the melted starch granules	767:792	the melted starch granules interpenetrated with the protein molecules	767:835	Moreover, the protein-starch synergistic effect on the storage modulus was strongest at fractions of 0.5 and 0.6, due to the formation of a semi-interpenetrating network, with more amylose from the melted starch granules interpenetrated with the protein molecules, and the absorption of water by the starch granules to concentrate the protein matrix.
27719902	2	8	theme	starch	511:516	arg1	matrix					518:523	starch matrix	511:523	starch matrix	511:523	The results revealed that the starch fraction of 0.5 was a critical point for the conversion of the protein matrix to starch matrix and conversion of the gel from elastic to weak.
27719902	1	9	theme	protein	364:370	arg1	structure					382:390	protein molecular structure	364:390	protein molecular structure	364:390	The changes in fish myofibrillar protein/cassava starch composites in the starch fraction range from 0 to 1, with their total content maintained at 60mg/mL, were investigated in terms of textural properties, rheological behaviours, morphology, spatial distribution and protein molecular structure.
27719902	1	10	with	composites	151:160	arg1	content					221:227	their total content	209:227	their total content maintained at 60mg/mL	209:249	The changes in fish myofibrillar protein/cassava starch composites in the starch fraction range from 0 to 1, with their total content maintained at 60mg/mL, were investigated in terms of textural properties, rheological behaviours, morphology, spatial distribution and protein molecular structure.
27719902	3	11	theme	starch	873:878	arg1	granules					880:887	the starch granules	869:887	the starch granules	869:887	Moreover, the protein-starch synergistic effect on the storage modulus was strongest at fractions of 0.5 and 0.6, due to the formation of a semi-interpenetrating network, with more amylose from the melted starch granules interpenetrated with the protein molecules, and the absorption of water by the starch granules to concentrate the protein matrix.
27719902	2	12	theme	matrix	501:506	arg1	conversion					475:484	the conversion	471:484	the conversion of the protein matrix to starch matrix and conversion of the gel from elastic to weak	471:570	The results revealed that the starch fraction of 0.5 was a critical point for the conversion of the protein matrix to starch matrix and conversion of the gel from elastic to weak.
27719902	1	13	theme	molecular	372:380	arg1	structure					382:390	protein molecular structure	364:390	protein molecular structure	364:390	The changes in fish myofibrillar protein/cassava starch composites in the starch fraction range from 0 to 1, with their total content maintained at 60mg/mL, were investigated in terms of textural properties, rheological behaviours, morphology, spatial distribution and protein molecular structure.
27719902	1	14	from	composites	151:160	arg1	1					201:201	1	201:201	1	201:201	The changes in fish myofibrillar protein/cassava starch composites in the starch fraction range from 0 to 1, with their total content maintained at 60mg/mL, were investigated in terms of textural properties, rheological behaviours, morphology, spatial distribution and protein molecular structure.
27719902	1	14	from	composites	151:160	arg1	range					185:189	the starch fraction range	165:189	the starch fraction range from 0 to 1	165:201	The changes in fish myofibrillar protein/cassava starch composites in the starch fraction range from 0 to 1, with their total content maintained at 60mg/mL, were investigated in terms of textural properties, rheological behaviours, morphology, spatial distribution and protein molecular structure.
27719902	3	15	theme	water	860:864	arg1	absorption					846:855	the absorption	842:855	the absorption of water by the starch granules	842:887	Moreover, the protein-starch synergistic effect on the storage modulus was strongest at fractions of 0.5 and 0.6, due to the formation of a semi-interpenetrating network, with more amylose from the melted starch granules interpenetrated with the protein molecules, and the absorption of water by the starch granules to concentrate the protein matrix.
27719902	3	15	theme	water	860:864	arg1	fractions					661:669	fractions	661:669	fractions of 0.5 and 0.6, due to the formation of a semi-interpenetrating network, with more amylose from the melted starch granules interpenetrated with the protein molecules,	661:836	Moreover, the protein-starch synergistic effect on the storage modulus was strongest at fractions of 0.5 and 0.6, due to the formation of a semi-interpenetrating network, with more amylose from the melted starch granules interpenetrated with the protein molecules, and the absorption of water by the starch granules to concentrate the protein matrix.
27719902	3	16	theme	network	735:741	arg1	formation					698:706	the formation	694:706	the formation of a semi-interpenetrating network	694:741	Moreover, the protein-starch synergistic effect on the storage modulus was strongest at fractions of 0.5 and 0.6, due to the formation of a semi-interpenetrating network, with more amylose from the melted starch granules interpenetrated with the protein molecules, and the absorption of water by the starch granules to concentrate the protein matrix.
27719902	1	17	theme	structure	382:390	arg1	terms					273:277	terms	273:277	terms of textural properties, rheological behaviours, morphology, spatial distribution and protein molecular structure	273:390	The changes in fish myofibrillar protein/cassava starch composites in the starch fraction range from 0 to 1, with their total content maintained at 60mg/mL, were investigated in terms of textural properties, rheological behaviours, morphology, spatial distribution and protein molecular structure.
27719902	2	18	theme	protein	493:499	arg1	matrix					501:506	the protein matrix	489:506	the protein matrix	489:506	The results revealed that the starch fraction of 0.5 was a critical point for the conversion of the protein matrix to starch matrix and conversion of the gel from elastic to weak.
27719902	3	19	from	effect	614:619	arg1	modulus					636:642	the storage modulus	624:642	the storage modulus	624:642	Moreover, the protein-starch synergistic effect on the storage modulus was strongest at fractions of 0.5 and 0.6, due to the formation of a semi-interpenetrating network, with more amylose from the melted starch granules interpenetrated with the protein molecules, and the absorption of water by the starch granules to concentrate the protein matrix.
27719902	3	20	theme	0.5	674:676	arg1	absorption					846:855	the absorption	842:855	the absorption of water by the starch granules	842:887	Moreover, the protein-starch synergistic effect on the storage modulus was strongest at fractions of 0.5 and 0.6, due to the formation of a semi-interpenetrating network, with more amylose from the melted starch granules interpenetrated with the protein molecules, and the absorption of water by the starch granules to concentrate the protein matrix.
27719902	3	20	theme	0.5	674:676	arg1	fractions					661:669	fractions	661:669	fractions of 0.5 and 0.6, due to the formation of a semi-interpenetrating network, with more amylose from the melted starch granules interpenetrated with the protein molecules,	661:836	Moreover, the protein-starch synergistic effect on the storage modulus was strongest at fractions of 0.5 and 0.6, due to the formation of a semi-interpenetrating network, with more amylose from the melted starch granules interpenetrated with the protein molecules, and the absorption of water by the starch granules to concentrate the protein matrix.
27719902	0	21	theme	Gel	0:2	arg1	characteristics					4:18	Gel characteristics	0:18	Gel characteristics	0:18	Gel characteristics and microstructure of fish myofibrillar protein/cassava starch composites.
27719902	4	22	theme	significant	1050:1060	arg1	influence					1062:1070	no significant influence	1047:1070	no significant influence on the protein secondary structure	1047:1105	Additionally, no covalent interaction between the protein and starch occurred with increasing starch fraction, thus having no significant influence on the protein secondary structure.
27719902	2	23	theme	0.5	442:444	arg1	point					461:465	a critical point	450:465	a critical point for the conversion of the protein matrix to starch matrix and conversion of the gel from elastic to weak	450:570	The results revealed that the starch fraction of 0.5 was a critical point for the conversion of the protein matrix to starch matrix and conversion of the gel from elastic to weak.
27719902	2	23	theme	0.5	442:444	arg1	fraction					430:437	the starch fraction	419:437	the starch fraction of 0.5	419:444	The results revealed that the starch fraction of 0.5 was a critical point for the conversion of the protein matrix to starch matrix and conversion of the gel from elastic to weak.
27719902	2	24	from	weak	567:570	arg1	conversion					529:538	conversion	529:538	conversion of the gel from elastic to weak	529:570	The results revealed that the starch fraction of 0.5 was a critical point for the conversion of the protein matrix to starch matrix and conversion of the gel from elastic to weak.
27719902	2	24	from	weak	567:570	arg1	gel					547:549	the gel	543:549	the gel from elastic to weak	543:570	The results revealed that the starch fraction of 0.5 was a critical point for the conversion of the protein matrix to starch matrix and conversion of the gel from elastic to weak.
27719902	2	24	from	weak	567:570	arg1	matrix					518:523	starch matrix	511:523	starch matrix	511:523	The results revealed that the starch fraction of 0.5 was a critical point for the conversion of the protein matrix to starch matrix and conversion of the gel from elastic to weak.
27719902	1	25	theme	fish	110:113	arg1	composites					151:160	fish myofibrillar protein/cassava starch composites	110:160	fish myofibrillar protein/cassava starch composites	110:160	The changes in fish myofibrillar protein/cassava starch composites in the starch fraction range from 0 to 1, with their total content maintained at 60mg/mL, were investigated in terms of textural properties, rheological behaviours, morphology, spatial distribution and protein molecular structure.
27719902	1	26	theme	textural	282:289	arg1	properties					291:300	textural properties	282:300	textural properties	282:300	The changes in fish myofibrillar protein/cassava starch composites in the starch fraction range from 0 to 1, with their total content maintained at 60mg/mL, were investigated in terms of textural properties, rheological behaviours, morphology, spatial distribution and protein molecular structure.
27719902	3	27	from	granules	785:792	arg1	amylose					754:760	more amylose	749:760	more amylose from the melted starch granules interpenetrated with the protein molecules	749:835	Moreover, the protein-starch synergistic effect on the storage modulus was strongest at fractions of 0.5 and 0.6, due to the formation of a semi-interpenetrating network, with more amylose from the melted starch granules interpenetrated with the protein molecules, and the absorption of water by the starch granules to concentrate the protein matrix.
27719902	3	28	theme	storage	628:634	arg1	modulus					636:642	the storage modulus	624:642	the storage modulus	624:642	Moreover, the protein-starch synergistic effect on the storage modulus was strongest at fractions of 0.5 and 0.6, due to the formation of a semi-interpenetrating network, with more amylose from the melted starch granules interpenetrated with the protein molecules, and the absorption of water by the starch granules to concentrate the protein matrix.
27719902	1	29	theme	myofibrillar	115:126	arg1	composites					151:160	fish myofibrillar protein/cassava starch composites	110:160	fish myofibrillar protein/cassava starch composites	110:160	The changes in fish myofibrillar protein/cassava starch composites in the starch fraction range from 0 to 1, with their total content maintained at 60mg/mL, were investigated in terms of textural properties, rheological behaviours, morphology, spatial distribution and protein molecular structure.
27719902	4	30	theme	secondary	1087:1095	arg1	structure					1097:1105	the protein secondary structure	1075:1105	the protein secondary structure	1075:1105	Additionally, no covalent interaction between the protein and starch occurred with increasing starch fraction, thus having no significant influence on the protein secondary structure.
27719902	2	31	theme	starch	423:428	arg1	point					461:465	a critical point	450:465	a critical point for the conversion of the protein matrix to starch matrix and conversion of the gel from elastic to weak	450:570	The results revealed that the starch fraction of 0.5 was a critical point for the conversion of the protein matrix to starch matrix and conversion of the gel from elastic to weak.
27719902	2	31	theme	starch	423:428	arg1	fraction					430:437	the starch fraction	419:437	the starch fraction of 0.5	419:444	The results revealed that the starch fraction of 0.5 was a critical point for the conversion of the protein matrix to starch matrix and conversion of the gel from elastic to weak.
27719902	0	32	theme	fish	42:45	arg1	composites					83:92	fish myofibrillar protein/cassava starch composites	42:92	fish myofibrillar protein/cassava starch composites	42:92	Gel characteristics and microstructure of fish myofibrillar protein/cassava starch composites.
27719902	1	33	theme	properties	291:300	arg1	terms					273:277	terms	273:277	terms of textural properties, rheological behaviours, morphology, spatial distribution and protein molecular structure	273:390	The changes in fish myofibrillar protein/cassava starch composites in the starch fraction range from 0 to 1, with their total content maintained at 60mg/mL, were investigated in terms of textural properties, rheological behaviours, morphology, spatial distribution and protein molecular structure.
27719902	4	34	theme	protein	1079:1085	arg1	structure					1097:1105	the protein secondary structure	1075:1105	the protein secondary structure	1075:1105	Additionally, no covalent interaction between the protein and starch occurred with increasing starch fraction, thus having no significant influence on the protein secondary structure.
27719902	2	35	dep	weak	567:570	arg1	to					564:565	to	564:565	to	564:565	The results revealed that the starch fraction of 0.5 was a critical point for the conversion of the protein matrix to starch matrix and conversion of the gel from elastic to weak.
27719902	1	36	dep	1	201:201	arg1	to					198:199	to	198:199	to	198:199	The changes in fish myofibrillar protein/cassava starch composites in the starch fraction range from 0 to 1, with their total content maintained at 60mg/mL, were investigated in terms of textural properties, rheological behaviours, morphology, spatial distribution and protein molecular structure.
27719902	1	37	theme	total	215:219	arg1	content					221:227	their total content	209:227	their total content maintained at 60mg/mL	209:249	The changes in fish myofibrillar protein/cassava starch composites in the starch fraction range from 0 to 1, with their total content maintained at 60mg/mL, were investigated in terms of textural properties, rheological behaviours, morphology, spatial distribution and protein molecular structure.
27719902	0	38	theme	protein/cassava	60:74	arg1	composites					83:92	fish myofibrillar protein/cassava starch composites	42:92	fish myofibrillar protein/cassava starch composites	42:92	Gel characteristics and microstructure of fish myofibrillar protein/cassava starch composites.
27719902	1	39	theme	rheological	303:313	arg1	behaviours					315:324	rheological behaviours	303:324	rheological behaviours	303:324	The changes in fish myofibrillar protein/cassava starch composites in the starch fraction range from 0 to 1, with their total content maintained at 60mg/mL, were investigated in terms of textural properties, rheological behaviours, morphology, spatial distribution and protein molecular structure.
27719902	3	40	theme	semi-interpenetrating	713:733	arg1	network					735:741	a semi-interpenetrating network	711:741	a semi-interpenetrating network	711:741	Moreover, the protein-starch synergistic effect on the storage modulus was strongest at fractions of 0.5 and 0.6, due to the formation of a semi-interpenetrating network, with more amylose from the melted starch granules interpenetrated with the protein molecules, and the absorption of water by the starch granules to concentrate the protein matrix.
27719902	1	41	theme	protein/cassava	128:142	arg1	composites					151:160	fish myofibrillar protein/cassava starch composites	110:160	fish myofibrillar protein/cassava starch composites	110:160	The changes in fish myofibrillar protein/cassava starch composites in the starch fraction range from 0 to 1, with their total content maintained at 60mg/mL, were investigated in terms of textural properties, rheological behaviours, morphology, spatial distribution and protein molecular structure.
27719902	0	42	theme	myofibrillar	47:58	arg1	composites					83:92	fish myofibrillar protein/cassava starch composites	42:92	fish myofibrillar protein/cassava starch composites	42:92	Gel characteristics and microstructure of fish myofibrillar protein/cassava starch composites.
27719902	1	43	theme	behaviours	315:324	arg1	terms					273:277	terms	273:277	terms of textural properties, rheological behaviours, morphology, spatial distribution and protein molecular structure	273:390	The changes in fish myofibrillar protein/cassava starch composites in the starch fraction range from 0 to 1, with their total content maintained at 60mg/mL, were investigated in terms of textural properties, rheological behaviours, morphology, spatial distribution and protein molecular structure.
27719902	2	44	theme	critical	452:459	arg1	point					461:465	a critical point	450:465	a critical point for the conversion of the protein matrix to starch matrix and conversion of the gel from elastic to weak	450:570	The results revealed that the starch fraction of 0.5 was a critical point for the conversion of the protein matrix to starch matrix and conversion of the gel from elastic to weak.
27719902	2	44	theme	critical	452:459	arg1	fraction					430:437	the starch fraction	419:437	the starch fraction of 0.5	419:444	The results revealed that the starch fraction of 0.5 was a critical point for the conversion of the protein matrix to starch matrix and conversion of the gel from elastic to weak.
27719902	3	45	theme	protein	908:914	arg1	matrix					916:921	the protein matrix	904:921	the protein matrix	904:921	Moreover, the protein-starch synergistic effect on the storage modulus was strongest at fractions of 0.5 and 0.6, due to the formation of a semi-interpenetrating network, with more amylose from the melted starch granules interpenetrated with the protein molecules, and the absorption of water by the starch granules to concentrate the protein matrix.
27719902	1	46	theme	starch	144:149	arg1	composites					151:160	fish myofibrillar protein/cassava starch composites	110:160	fish myofibrillar protein/cassava starch composites	110:160	The changes in fish myofibrillar protein/cassava starch composites in the starch fraction range from 0 to 1, with their total content maintained at 60mg/mL, were investigated in terms of textural properties, rheological behaviours, morphology, spatial distribution and protein molecular structure.
27719902	3	47	theme	synergistic	602:612	arg1	strongest					648:656	strongest	648:656	strongest	648:656	Moreover, the protein-starch synergistic effect on the storage modulus was strongest at fractions of 0.5 and 0.6, due to the formation of a semi-interpenetrating network, with more amylose from the melted starch granules interpenetrated with the protein molecules, and the absorption of water by the starch granules to concentrate the protein matrix.
27719902	3	47	theme	synergistic	602:612	arg1	effect					614:619	the protein-starch synergistic effect	583:619	the protein-starch synergistic effect on the storage modulus	583:642	Moreover, the protein-starch synergistic effect on the storage modulus was strongest at fractions of 0.5 and 0.6, due to the formation of a semi-interpenetrating network, with more amylose from the melted starch granules interpenetrated with the protein molecules, and the absorption of water by the starch granules to concentrate the protein matrix.
27719902	2	48	from	conversion	529:538	arg1	weak					567:570	weak	567:570	weak	567:570	The results revealed that the starch fraction of 0.5 was a critical point for the conversion of the protein matrix to starch matrix and conversion of the gel from elastic to weak.
27719902	2	49	theme	gel	547:549	arg1	conversion					529:538	conversion	529:538	conversion of the gel from elastic to weak	529:570	The results revealed that the starch fraction of 0.5 was a critical point for the conversion of the protein matrix to starch matrix and conversion of the gel from elastic to weak.
27719902	2	49	theme	gel	547:549	arg1	matrix					518:523	starch matrix	511:523	starch matrix	511:523	The results revealed that the starch fraction of 0.5 was a critical point for the conversion of the protein matrix to starch matrix and conversion of the gel from elastic to weak.
27719902	2	50	from	matrix	518:523	arg1	weak					567:570	weak	567:570	weak	567:570	The results revealed that the starch fraction of 0.5 was a critical point for the conversion of the protein matrix to starch matrix and conversion of the gel from elastic to weak.
27719902	1	51	from	changes	99:105	arg1	composites					151:160	fish myofibrillar protein/cassava starch composites	110:160	fish myofibrillar protein/cassava starch composites	110:160	The changes in fish myofibrillar protein/cassava starch composites in the starch fraction range from 0 to 1, with their total content maintained at 60mg/mL, were investigated in terms of textural properties, rheological behaviours, morphology, spatial distribution and protein molecular structure.
27719902	3	52	theme	melted	771:776	arg1	granules					785:792	the melted starch granules	767:792	the melted starch granules interpenetrated with the protein molecules	767:835	Moreover, the protein-starch synergistic effect on the storage modulus was strongest at fractions of 0.5 and 0.6, due to the formation of a semi-interpenetrating network, with more amylose from the melted starch granules interpenetrated with the protein molecules, and the absorption of water by the starch granules to concentrate the protein matrix.
27719902	0	53	theme	composites	83:92	arg1	microstructure					24:37	microstructure	24:37	microstructure	24:37	Gel characteristics and microstructure of fish myofibrillar protein/cassava starch composites.
27719902	0	53	theme	composites	83:92	arg1	characteristics					4:18	Gel characteristics	0:18	Gel characteristics	0:18	Gel characteristics and microstructure of fish myofibrillar protein/cassava starch composites.
27719902	4	54	from	influence	1062:1070	arg1	structure					1097:1105	the protein secondary structure	1075:1105	the protein secondary structure	1075:1105	Additionally, no covalent interaction between the protein and starch occurred with increasing starch fraction, thus having no significant influence on the protein secondary structure.
27719902	1	55	from	1	201:201	arg1	composites					151:160	fish myofibrillar protein/cassava starch composites	110:160	fish myofibrillar protein/cassava starch composites	110:160	The changes in fish myofibrillar protein/cassava starch composites in the starch fraction range from 0 to 1, with their total content maintained at 60mg/mL, were investigated in terms of textural properties, rheological behaviours, morphology, spatial distribution and protein molecular structure.
27719902	1	55	from	1	201:201	arg1	range					185:189	the starch fraction range	165:189	the starch fraction range from 0 to 1	165:201	The changes in fish myofibrillar protein/cassava starch composites in the starch fraction range from 0 to 1, with their total content maintained at 60mg/mL, were investigated in terms of textural properties, rheological behaviours, morphology, spatial distribution and protein molecular structure.
27719902	3	56	theme	protein	819:825	arg1	molecules					827:835	the protein molecules	815:835	the protein molecules	815:835	Moreover, the protein-starch synergistic effect on the storage modulus was strongest at fractions of 0.5 and 0.6, due to the formation of a semi-interpenetrating network, with more amylose from the melted starch granules interpenetrated with the protein molecules, and the absorption of water by the starch granules to concentrate the protein matrix.
27719902	0	57	theme	starch	76:81	arg1	composites					83:92	fish myofibrillar protein/cassava starch composites	42:92	fish myofibrillar protein/cassava starch composites	42:92	Gel characteristics and microstructure of fish myofibrillar protein/cassava starch composites.
27719902	1	58	theme	morphology	327:336	arg1	terms					273:277	terms	273:277	terms of textural properties, rheological behaviours, morphology, spatial distribution and protein molecular structure	273:390	The changes in fish myofibrillar protein/cassava starch composites in the starch fraction range from 0 to 1, with their total content maintained at 60mg/mL, were investigated in terms of textural properties, rheological behaviours, morphology, spatial distribution and protein molecular structure.
27719902	3	59	theme	protein-starch	587:600	arg1	strongest					648:656	strongest	648:656	strongest	648:656	Moreover, the protein-starch synergistic effect on the storage modulus was strongest at fractions of 0.5 and 0.6, due to the formation of a semi-interpenetrating network, with more amylose from the melted starch granules interpenetrated with the protein molecules, and the absorption of water by the starch granules to concentrate the protein matrix.
27719902	3	59	theme	protein-starch	587:600	arg1	effect					614:619	the protein-starch synergistic effect	583:619	the protein-starch synergistic effect on the storage modulus	583:642	Moreover, the protein-starch synergistic effect on the storage modulus was strongest at fractions of 0.5 and 0.6, due to the formation of a semi-interpenetrating network, with more amylose from the melted starch granules interpenetrated with the protein molecules, and the absorption of water by the starch granules to concentrate the protein matrix.
27719902	1	60	theme	starch	169:174	arg1	range					185:189	the starch fraction range	165:189	the starch fraction range from 0 to 1	165:201	The changes in fish myofibrillar protein/cassava starch composites in the starch fraction range from 0 to 1, with their total content maintained at 60mg/mL, were investigated in terms of textural properties, rheological behaviours, morphology, spatial distribution and protein molecular structure.
27719902	3	61	theme	more	749:752	arg1	amylose					754:760	more amylose	749:760	more amylose from the melted starch granules interpenetrated with the protein molecules	749:835	Moreover, the protein-starch synergistic effect on the storage modulus was strongest at fractions of 0.5 and 0.6, due to the formation of a semi-interpenetrating network, with more amylose from the melted starch granules interpenetrated with the protein molecules, and the absorption of water by the starch granules to concentrate the protein matrix.
28267519	2	0	theme	electron	396:403	arg1	FE-SEM					417:422	FE-SEM	417:422	FE-SEM	417:422	PAni was densely arrayed along BC fibers and SWCNTs were uniformly distributed in the composites as confirmed by field emission scanning electron microscopy (FE-SEM).
28267519	2	0	theme	electron	396:403	arg1	microscopy					405:414	field emission scanning electron microscopy	372:414	field emission scanning electron microscopy (FE-SEM)	372:423	PAni was densely arrayed along BC fibers and SWCNTs were uniformly distributed in the composites as confirmed by field emission scanning electron microscopy (FE-SEM).
28267519	5	1	from	232°C	807:811	arg1	temperatures					789:800	the degradation temperatures	773:800	the degradation temperatures from 232°C for BC to 260°C, 302°C, and 310°C for BC-PAni, BC-PAni/SWCNTs-I (0.05mg/mL), and BC-PAni/SWCNTs-II (0.1mg/mL) composites, respectively	773:946	The conjugated backbone of PAni and SWCNTs contributed to improve the degradation temperatures from 232°C for BC to 260°C, 302°C, and 310°C for BC-PAni, BC-PAni/SWCNTs-I (0.05mg/mL), and BC-PAni/SWCNTs-II (0.1mg/mL) composites, respectively.
28267519	5	2	dep	232°C	807:811	arg1	to					820:821	to	820:821	to	820:821	The conjugated backbone of PAni and SWCNTs contributed to improve the degradation temperatures from 232°C for BC to 260°C, 302°C, and 310°C for BC-PAni, BC-PAni/SWCNTs-I (0.05mg/mL), and BC-PAni/SWCNTs-II (0.1mg/mL) composites, respectively.
28267519	5	2	dep	232°C	807:811	arg1	302°C					830:834	302°C	830:834	302°C	830:834	The conjugated backbone of PAni and SWCNTs contributed to improve the degradation temperatures from 232°C for BC to 260°C, 302°C, and 310°C for BC-PAni, BC-PAni/SWCNTs-I (0.05mg/mL), and BC-PAni/SWCNTs-II (0.1mg/mL) composites, respectively.
28267519	5	2	dep	232°C	807:811	arg1	310°C					841:845	310°C	841:845	310°C	841:845	The conjugated backbone of PAni and SWCNTs contributed to improve the degradation temperatures from 232°C for BC to 260°C, 302°C, and 310°C for BC-PAni, BC-PAni/SWCNTs-I (0.05mg/mL), and BC-PAni/SWCNTs-II (0.1mg/mL) composites, respectively.
28267519	5	2	dep	232°C	807:811	arg1	260°C					823:827	260°C	823:827	260°C	823:827	The conjugated backbone of PAni and SWCNTs contributed to improve the degradation temperatures from 232°C for BC to 260°C, 302°C, and 310°C for BC-PAni, BC-PAni/SWCNTs-I (0.05mg/mL), and BC-PAni/SWCNTs-II (0.1mg/mL) composites, respectively.
28267519	3	3	theme	PAni	561:564	arg1	PAni					561:564	PAni	561:564	PAni	561:564	Fourier transform-infrared (FT-IR) spectra of the composite membranes exhibited characteristic peaks for specific functional groups of PAni and SWCNTs besides BC.
28267519	3	3	theme	PAni	561:564	arg1	groups					551:556	specific functional groups	531:556	specific functional groups of PAni and SWCNTs besides BC	531:586	Fourier transform-infrared (FT-IR) spectra of the composite membranes exhibited characteristic peaks for specific functional groups of PAni and SWCNTs besides BC.
28267519	3	3	theme	PAni	561:564	arg1	SWCNTs					570:575	SWCNTs	570:575	SWCNTs	570:575	Fourier transform-infrared (FT-IR) spectra of the composite membranes exhibited characteristic peaks for specific functional groups of PAni and SWCNTs besides BC.
28267519	4	4	from	presence	636:643	arg1	composites					695:704	the composites	691:704	the composites	691:704	X-ray diffraction (XRD) analysis indicated the presence of specific peaks for BC, PAni, and SWCNTs in the composites.
28267519	7	5	theme	electrical	1344:1353	arg1	conductivity					1355:1366	electrical conductivity	1344:1366	electrical conductivity	1344:1366	These membranes can potentially be used for applications requiring biocompatibility and electrical conductivity such as biological and chemical sensors.
28267519	2	6	theme	scanning	387:394	arg1	FE-SEM					417:422	FE-SEM	417:422	FE-SEM	417:422	PAni was densely arrayed along BC fibers and SWCNTs were uniformly distributed in the composites as confirmed by field emission scanning electron microscopy (FE-SEM).
28267519	2	6	theme	scanning	387:394	arg1	microscopy					405:414	field emission scanning electron microscopy	372:414	field emission scanning electron microscopy (FE-SEM)	372:423	PAni was densely arrayed along BC fibers and SWCNTs were uniformly distributed in the composites as confirmed by field emission scanning electron microscopy (FE-SEM).
28267519	6	7	theme	0.1mg/mL	1088:1095	arg1	SWCNTs					1097:1102	0.05mg/mL and 0.1mg/mL SWCNTs	1074:1102	SWCNTs	1097:1102	The electrical conductivity of BC was enhanced to 1.04×10-3S/cm, 4.64×10-3S/cm, and 1.41×10-2S/cm upon doping with PAni, and 0.05mg/mL and 0.1mg/mL SWCNTs, respectively in dry state which was further increased to 4.02×10-2S/cm, 3.03×10-2S/cm, 5.93×10-1S/cm, and 7.36×10-1S/cm, respectively in PBS solution.
28267519	3	8	theme	functional	540:549	arg1	PAni					561:564	PAni	561:564	PAni	561:564	Fourier transform-infrared (FT-IR) spectra of the composite membranes exhibited characteristic peaks for specific functional groups of PAni and SWCNTs besides BC.
28267519	3	8	theme	functional	540:549	arg1	groups					551:556	specific functional groups	531:556	specific functional groups of PAni and SWCNTs besides BC	531:586	Fourier transform-infrared (FT-IR) spectra of the composite membranes exhibited characteristic peaks for specific functional groups of PAni and SWCNTs besides BC.
28267519	3	8	theme	functional	540:549	arg1	SWCNTs					570:575	SWCNTs	570:575	SWCNTs	570:575	Fourier transform-infrared (FT-IR) spectra of the composite membranes exhibited characteristic peaks for specific functional groups of PAni and SWCNTs besides BC.
28267519	6	9	theme	0.05mg/mL	1074:1082	arg1	SWCNTs					1097:1102	0.05mg/mL and 0.1mg/mL SWCNTs	1074:1102	SWCNTs	1097:1102	The electrical conductivity of BC was enhanced to 1.04×10-3S/cm, 4.64×10-3S/cm, and 1.41×10-2S/cm upon doping with PAni, and 0.05mg/mL and 0.1mg/mL SWCNTs, respectively in dry state which was further increased to 4.02×10-2S/cm, 3.03×10-2S/cm, 5.93×10-1S/cm, and 7.36×10-1S/cm, respectively in PBS solution.
28267519	5	10	theme	BC-PAni/SWCNTs-II	894:910	arg1	composites					923:932	BC-PAni/SWCNTs-II (0.1mg/mL) composites	894:932	BC-PAni/SWCNTs-II (0.1mg/mL) composites	894:932	The conjugated backbone of PAni and SWCNTs contributed to improve the degradation temperatures from 232°C for BC to 260°C, 302°C, and 310°C for BC-PAni, BC-PAni/SWCNTs-I (0.05mg/mL), and BC-PAni/SWCNTs-II (0.1mg/mL) composites, respectively.
28267519	3	11	theme	composite	476:484	arg1	membranes					486:494	the composite membranes	472:494	the composite membranes	472:494	Fourier transform-infrared (FT-IR) spectra of the composite membranes exhibited characteristic peaks for specific functional groups of PAni and SWCNTs besides BC.
28267519	6	12	theme	electrical	953:962	arg1	conductivity					964:975	The electrical conductivity	949:975	The electrical conductivity of BC	949:981	The electrical conductivity of BC was enhanced to 1.04×10-3S/cm, 4.64×10-3S/cm, and 1.41×10-2S/cm upon doping with PAni, and 0.05mg/mL and 0.1mg/mL SWCNTs, respectively in dry state which was further increased to 4.02×10-2S/cm, 3.03×10-2S/cm, 5.93×10-1S/cm, and 7.36×10-1S/cm, respectively in PBS solution.
28267519	1	13	with	membranes	161:169	arg1	PAni					195:198	PAni	195:198	PAni	195:198	Electrically conductive polymeric membranes of BC with polyaniline (PAni) were fabricated through ex situ oxidative polymerization.
28267519	1	13	with	membranes	161:169	arg1	polyaniline					182:192	polyaniline	182:192	polyaniline (PAni)	182:199	Electrically conductive polymeric membranes of BC with polyaniline (PAni) were fabricated through ex situ oxidative polymerization.
28267519	3	14	theme	Fourier	426:432	arg1	spectra					461:467	Fourier transform-infrared (FT-IR) spectra	426:467	Fourier transform-infrared (FT-IR) spectra of the composite membranes	426:494	Fourier transform-infrared (FT-IR) spectra of the composite membranes exhibited characteristic peaks for specific functional groups of PAni and SWCNTs besides BC.
28267519	6	15	theme	BC	980:981	arg1	conductivity					964:975	The electrical conductivity	949:975	The electrical conductivity of BC	949:981	The electrical conductivity of BC was enhanced to 1.04×10-3S/cm, 4.64×10-3S/cm, and 1.41×10-2S/cm upon doping with PAni, and 0.05mg/mL and 0.1mg/mL SWCNTs, respectively in dry state which was further increased to 4.02×10-2S/cm, 3.03×10-2S/cm, 5.93×10-1S/cm, and 7.36×10-1S/cm, respectively in PBS solution.
28267519	6	16	theme	PBS	1242:1244	arg1	solution					1246:1253	PBS solution	1242:1253	PBS solution	1242:1253	The electrical conductivity of BC was enhanced to 1.04×10-3S/cm, 4.64×10-3S/cm, and 1.41×10-2S/cm upon doping with PAni, and 0.05mg/mL and 0.1mg/mL SWCNTs, respectively in dry state which was further increased to 4.02×10-2S/cm, 3.03×10-2S/cm, 5.93×10-1S/cm, and 7.36×10-1S/cm, respectively in PBS solution.
28267519	7	17	used	used	1291:1294	arg2	membranes					1262:1270	These membranes	1256:1270	These membranes	1256:1270	These membranes can potentially be used for applications requiring biocompatibility and electrical conductivity such as biological and chemical sensors.
28267519	1	18	theme	ex	225:226	arg1	polymerization					243:256	ex situ oxidative polymerization	225:256	ex situ oxidative polymerization	225:256	Electrically conductive polymeric membranes of BC with polyaniline (PAni) were fabricated through ex situ oxidative polymerization.
28267519	0	19	theme	cellulose/polyaniline/single-walled	25:59	arg1	carbon					61:66	bacterial cellulose/polyaniline/single-walled carbon	15:66	bacterial cellulose/polyaniline/single-walled carbon	15:66	Fabrication of bacterial cellulose/polyaniline/single-walled carbon nanotubes membrane for potential application as biosensor.
28267519	4	20	theme	diffraction	595:605	arg1	analysis					613:620	X-ray diffraction (XRD) analysis	589:620	X-ray diffraction (XRD) analysis	589:620	X-ray diffraction (XRD) analysis indicated the presence of specific peaks for BC, PAni, and SWCNTs in the composites.
28267519	1	21	theme	conductive	140:149	arg1	membranes					161:169	Electrically conductive polymeric membranes	127:169	Electrically conductive polymeric membranes of BC with polyaniline (PAni)	127:199	Electrically conductive polymeric membranes of BC with polyaniline (PAni) were fabricated through ex situ oxidative polymerization.
28267519	3	22	theme	characteristic	506:519	arg1	peaks					521:525	characteristic peaks	506:525	characteristic peaks for specific functional groups of PAni and SWCNTs besides BC	506:586	Fourier transform-infrared (FT-IR) spectra of the composite membranes exhibited characteristic peaks for specific functional groups of PAni and SWCNTs besides BC.
28267519	0	23	theme	bacterial	15:23	arg1	carbon					61:66	bacterial cellulose/polyaniline/single-walled carbon	15:66	bacterial cellulose/polyaniline/single-walled carbon	15:66	Fabrication of bacterial cellulose/polyaniline/single-walled carbon nanotubes membrane for potential application as biosensor.
28267519	1	24	theme	polymeric	151:159	arg1	membranes					161:169	Electrically conductive polymeric membranes	127:169	Electrically conductive polymeric membranes of BC with polyaniline (PAni)	127:199	Electrically conductive polymeric membranes of BC with polyaniline (PAni) were fabricated through ex situ oxidative polymerization.
28267519	1	25	theme	oxidative	233:241	arg1	polymerization					243:256	ex situ oxidative polymerization	225:256	ex situ oxidative polymerization	225:256	Electrically conductive polymeric membranes of BC with polyaniline (PAni) were fabricated through ex situ oxidative polymerization.
28267519	3	26	theme	FT-IR	454:458	arg1	spectra					461:467	Fourier transform-infrared (FT-IR) spectra	426:467	Fourier transform-infrared (FT-IR) spectra of the composite membranes	426:494	Fourier transform-infrared (FT-IR) spectra of the composite membranes exhibited characteristic peaks for specific functional groups of PAni and SWCNTs besides BC.
28267519	5	27	theme	0.1mg/mL	913:920	arg1	composites					923:932	BC-PAni/SWCNTs-II (0.1mg/mL) composites	894:932	BC-PAni/SWCNTs-II (0.1mg/mL) composites	894:932	The conjugated backbone of PAni and SWCNTs contributed to improve the degradation temperatures from 232°C for BC to 260°C, 302°C, and 310°C for BC-PAni, BC-PAni/SWCNTs-I (0.05mg/mL), and BC-PAni/SWCNTs-II (0.1mg/mL) composites, respectively.
28267519	5	28	theme	PAni	734:737	arg1	backbone					722:729	The conjugated backbone	707:729	The conjugated backbone of PAni and SWCNTs	707:748	The conjugated backbone of PAni and SWCNTs contributed to improve the degradation temperatures from 232°C for BC to 260°C, 302°C, and 310°C for BC-PAni, BC-PAni/SWCNTs-I (0.05mg/mL), and BC-PAni/SWCNTs-II (0.1mg/mL) composites, respectively.
28267519	6	29	theme	dry	1121:1123	arg1	state					1125:1129	dry state	1121:1129	dry state which was further increased to 4.02×10-2S/cm, 3.03×10-2S/cm, 5.93×10-1S/cm, and 7.36×10-1S/cm, respectively in PBS solution	1121:1253	The electrical conductivity of BC was enhanced to 1.04×10-3S/cm, 4.64×10-3S/cm, and 1.41×10-2S/cm upon doping with PAni, and 0.05mg/mL and 0.1mg/mL SWCNTs, respectively in dry state which was further increased to 4.02×10-2S/cm, 3.03×10-2S/cm, 5.93×10-1S/cm, and 7.36×10-1S/cm, respectively in PBS solution.
28267519	3	30	theme	membranes	486:494	arg1	spectra					461:467	Fourier transform-infrared (FT-IR) spectra	426:467	Fourier transform-infrared (FT-IR) spectra of the composite membranes	426:494	Fourier transform-infrared (FT-IR) spectra of the composite membranes exhibited characteristic peaks for specific functional groups of PAni and SWCNTs besides BC.
28267519	0	31	theme	carbon	61:66	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of bacterial cellulose/polyaniline/single-walled carbon	0:66	Fabrication of bacterial cellulose/polyaniline/single-walled carbon nanotubes membrane for potential application as biosensor.
28267519	1	32	dep	ex	225:226	arg1	situ					228:231	situ	228:231	situ	228:231	Electrically conductive polymeric membranes of BC with polyaniline (PAni) were fabricated through ex situ oxidative polymerization.
28267519	3	33	theme	transform-infrared	434:451	arg1	spectra					461:467	Fourier transform-infrared (FT-IR) spectra	426:467	Fourier transform-infrared (FT-IR) spectra of the composite membranes	426:494	Fourier transform-infrared (FT-IR) spectra of the composite membranes exhibited characteristic peaks for specific functional groups of PAni and SWCNTs besides BC.
28267519	5	34	theme	SWCNTs	743:748	arg1	backbone					722:729	The conjugated backbone	707:729	The conjugated backbone of PAni and SWCNTs	707:748	The conjugated backbone of PAni and SWCNTs contributed to improve the degradation temperatures from 232°C for BC to 260°C, 302°C, and 310°C for BC-PAni, BC-PAni/SWCNTs-I (0.05mg/mL), and BC-PAni/SWCNTs-II (0.1mg/mL) composites, respectively.
28267519	5	35	theme	degradation	777:787	arg1	temperatures					789:800	the degradation temperatures	773:800	the degradation temperatures from 232°C for BC to 260°C, 302°C, and 310°C for BC-PAni, BC-PAni/SWCNTs-I (0.05mg/mL), and BC-PAni/SWCNTs-II (0.1mg/mL) composites, respectively	773:946	The conjugated backbone of PAni and SWCNTs contributed to improve the degradation temperatures from 232°C for BC to 260°C, 302°C, and 310°C for BC-PAni, BC-PAni/SWCNTs-I (0.05mg/mL), and BC-PAni/SWCNTs-II (0.1mg/mL) composites, respectively.
28267519	7	36	theme	chemical	1391:1398	arg1	sensors					1400:1406	biological and chemical sensors	1376:1406	biological and chemical sensors	1376:1406	These membranes can potentially be used for applications requiring biocompatibility and electrical conductivity such as biological and chemical sensors.
28267519	2	37	theme	emission	378:385	arg1	FE-SEM					417:422	FE-SEM	417:422	FE-SEM	417:422	PAni was densely arrayed along BC fibers and SWCNTs were uniformly distributed in the composites as confirmed by field emission scanning electron microscopy (FE-SEM).
28267519	2	37	theme	emission	378:385	arg1	microscopy					405:414	field emission scanning electron microscopy	372:414	field emission scanning electron microscopy (FE-SEM)	372:423	PAni was densely arrayed along BC fibers and SWCNTs were uniformly distributed in the composites as confirmed by field emission scanning electron microscopy (FE-SEM).
28267519	4	38	theme	X-ray	589:593	arg1	XRD					608:610	XRD	608:610	XRD	608:610	X-ray diffraction (XRD) analysis indicated the presence of specific peaks for BC, PAni, and SWCNTs in the composites.
28267519	4	38	theme	X-ray	589:593	arg1	diffraction					595:605	X-ray diffraction	589:605	X-ray diffraction (XRD) analysis	589:620	X-ray diffraction (XRD) analysis indicated the presence of specific peaks for BC, PAni, and SWCNTs in the composites.
28267519	2	39	theme	field	372:376	arg1	FE-SEM					417:422	FE-SEM	417:422	FE-SEM	417:422	PAni was densely arrayed along BC fibers and SWCNTs were uniformly distributed in the composites as confirmed by field emission scanning electron microscopy (FE-SEM).
28267519	2	39	theme	field	372:376	arg1	microscopy					405:414	field emission scanning electron microscopy	372:414	field emission scanning electron microscopy (FE-SEM)	372:423	PAni was densely arrayed along BC fibers and SWCNTs were uniformly distributed in the composites as confirmed by field emission scanning electron microscopy (FE-SEM).
28267519	7	40	theme	biological	1376:1385	arg1	sensors					1400:1406	biological and chemical sensors	1376:1406	biological and chemical sensors	1376:1406	These membranes can potentially be used for applications requiring biocompatibility and electrical conductivity such as biological and chemical sensors.
28267519	1	41	theme	BC	174:175	arg1	membranes					161:169	Electrically conductive polymeric membranes	127:169	Electrically conductive polymeric membranes of BC with polyaniline (PAni)	127:199	Electrically conductive polymeric membranes of BC with polyaniline (PAni) were fabricated through ex situ oxidative polymerization.
28267519	4	42	theme	specific	648:655	arg1	peaks					657:661	specific peaks	648:661	specific peaks for BC, PAni, and SWCNTs	648:686	X-ray diffraction (XRD) analysis indicated the presence of specific peaks for BC, PAni, and SWCNTs in the composites.
28267519	5	43	theme	conjugated	711:720	arg1	backbone					722:729	The conjugated backbone	707:729	The conjugated backbone of PAni and SWCNTs	707:748	The conjugated backbone of PAni and SWCNTs contributed to improve the degradation temperatures from 232°C for BC to 260°C, 302°C, and 310°C for BC-PAni, BC-PAni/SWCNTs-I (0.05mg/mL), and BC-PAni/SWCNTs-II (0.1mg/mL) composites, respectively.
28267519	3	44	theme	specific	531:538	arg1	PAni					561:564	PAni	561:564	PAni	561:564	Fourier transform-infrared (FT-IR) spectra of the composite membranes exhibited characteristic peaks for specific functional groups of PAni and SWCNTs besides BC.
28267519	3	44	theme	specific	531:538	arg1	groups					551:556	specific functional groups	531:556	specific functional groups of PAni and SWCNTs besides BC	531:586	Fourier transform-infrared (FT-IR) spectra of the composite membranes exhibited characteristic peaks for specific functional groups of PAni and SWCNTs besides BC.
28267519	3	44	theme	specific	531:538	arg1	SWCNTs					570:575	SWCNTs	570:575	SWCNTs	570:575	Fourier transform-infrared (FT-IR) spectra of the composite membranes exhibited characteristic peaks for specific functional groups of PAni and SWCNTs besides BC.
28267519	2	45	theme	BC	290:291	arg1	fibers					293:298	BC fibers	290:298	BC fibers	290:298	PAni was densely arrayed along BC fibers and SWCNTs were uniformly distributed in the composites as confirmed by field emission scanning electron microscopy (FE-SEM).
28267519	0	46	theme	potential	91:99	arg1	application					101:111	potential application	91:111	potential application as biosensor	91:124	Fabrication of bacterial cellulose/polyaniline/single-walled carbon nanotubes membrane for potential application as biosensor.
28267519	4	47	attach	presence	636:643	arg1	composites					695:704	the composites	691:704	the composites	691:704	X-ray diffraction (XRD) analysis indicated the presence of specific peaks for BC, PAni, and SWCNTs in the composites.
28267519	4	47	attach	presence	636:643	arg2	peaks					657:661	specific peaks	648:661	specific peaks for BC, PAni, and SWCNTs	648:686	X-ray diffraction (XRD) analysis indicated the presence of specific peaks for BC, PAni, and SWCNTs in the composites.
28267519	3	48	theme	SWCNTs	570:575	arg1	PAni					561:564	PAni	561:564	PAni	561:564	Fourier transform-infrared (FT-IR) spectra of the composite membranes exhibited characteristic peaks for specific functional groups of PAni and SWCNTs besides BC.
28267519	3	48	theme	SWCNTs	570:575	arg1	groups					551:556	specific functional groups	531:556	specific functional groups of PAni and SWCNTs besides BC	531:586	Fourier transform-infrared (FT-IR) spectra of the composite membranes exhibited characteristic peaks for specific functional groups of PAni and SWCNTs besides BC.
28267519	3	48	theme	SWCNTs	570:575	arg1	SWCNTs					570:575	SWCNTs	570:575	SWCNTs	570:575	Fourier transform-infrared (FT-IR) spectra of the composite membranes exhibited characteristic peaks for specific functional groups of PAni and SWCNTs besides BC.
28267519	4	49	theme	peaks	657:661	arg1	presence					636:643	the presence	632:643	the presence of specific peaks for BC, PAni, and SWCNTs in the composites	632:704	X-ray diffraction (XRD) analysis indicated the presence of specific peaks for BC, PAni, and SWCNTs in the composites.
28602514	0	0	theme	proteoglycan	72:83	arg1	expression					85:94	proteoglycan expression	72:94	proteoglycan expression	72:94	A jasmonic acid derivative improves skin healing and induces changes in proteoglycan expression and glycosaminoglycan structure.
28602514	11	1	theme	promising	1681:1689	arg1	JAD					1672:1674	JAD	1672:1674	JAD	1672:1674	CONCLUSION This study highlights JAD as a promising compound for investigating GAG structure-function relationships and for applications in skin cosmetic /corrective strategies.
28602514	11	1	theme	promising	1681:1689	arg1	compound					1691:1698	a promising compound	1679:1698	a promising compound for investigating GAG structure-function relationships and for applications in skin cosmetic /corrective strategies	1679:1814	CONCLUSION This study highlights JAD as a promising compound for investigating GAG structure-function relationships and for applications in skin cosmetic /corrective strategies.
28602514	5	2	theme	factor	902:907	arg1	activities					909:918	growth factor activities	895:918	growth factor activities	895:918	In parallel, we have examined the ability of JAD to promote growth factor activities and improve skin wound healing.
28602514	9	3	theme	major	1451:1455	arg1	proteoglycans					1462:1474	major skin proteoglycans	1451:1474	major skin proteoglycans	1451:1474	RESULTS JAD was found to induce expression of major skin proteoglycans and to induce subtle changes in GAG structure.
28602514	8	4	theme	blister	1355:1361	arg1	model					1363:1367	a suction blister model	1345:1367	a suction blister model performed on 24 healthy volunteers	1345:1402	Finally, biological activities of JAD were assessed in cellulo, in FGF-7 induced migration/proliferation assays, as well as in vivo, using a suction blister model performed on 24 healthy volunteers.
28602514	7	5	theme	disaccharide	1183:1194	arg1	analysis					1196:1203	disaccharide analysis	1183:1203	disaccharide analysis	1183:1203	GAG composition was determined by disaccharide analysis.
28602514	3	6	theme	human	538:542	arg1	skin					544:547	human skin	538:547	human skin	538:547	Recently, we have reported that a jasmonate derivative (JAD) displayed anti-aging effects on human skin by inducing extracellular matrix (ECM) remodeling.
28602514	9	7	theme	skin	1457:1460	arg1	proteoglycans					1462:1474	major skin proteoglycans	1451:1474	major skin proteoglycans	1451:1474	RESULTS JAD was found to induce expression of major skin proteoglycans and to induce subtle changes in GAG structure.
28602514	10	8	theme	epithelial	1612:1621	arg1	repair					1623:1628	epithelial repair	1612:1628	epithelial repair	1612:1628	In parallel, we showed that JAD promoted FGF-7 and improved skin healing by accelerating epithelial repair in vivo.
28602514	3	9	theme	matrix	575:580	arg1	remodeling					588:597	extracellular matrix (ECM) remodeling	561:597	extracellular matrix (ECM) remodeling	561:597	Recently, we have reported that a jasmonate derivative (JAD) displayed anti-aging effects on human skin by inducing extracellular matrix (ECM) remodeling.
28602514	12	10	dep	SIGNIFICANCE	1825:1836	arg1	We					1838:1839	We	1838:1839	We	1838:1839	GENERAL SIGNIFICANCE We propose here a novel mechanism, by which jasmonate derivatives may elicit biological activities in mammals.
28602514	6	11	theme	primary	1010:1016	arg1	keratinocytes					1018:1030	epidermal primary keratinocytes	1000:1030	epidermal primary keratinocytes	1000:1030	METHODS Proteoglycan expression was analyzed on epidermal primary keratinocytes and reconstituted skin epidermis, using electron/immunofluorescence microscopy, western blotting and flow cytometry.
28602514	8	12	theme	biological	1215:1224	arg1	activities					1226:1235	biological activities	1215:1235	biological activities of JAD	1215:1242	Finally, biological activities of JAD were assessed in cellulo, in FGF-7 induced migration/proliferation assays, as well as in vivo, using a suction blister model performed on 24 healthy volunteers.
28602514	6	13	theme	epidermal	1000:1008	arg1	keratinocytes					1018:1030	epidermal primary keratinocytes	1000:1030	epidermal primary keratinocytes	1000:1030	METHODS Proteoglycan expression was analyzed on epidermal primary keratinocytes and reconstituted skin epidermis, using electron/immunofluorescence microscopy, western blotting and flow cytometry.
28602514	8	14	dep	in	1330:1331	arg1	vivo					1333:1336	vivo	1333:1336	vivo	1333:1336	Finally, biological activities of JAD were assessed in cellulo, in FGF-7 induced migration/proliferation assays, as well as in vivo, using a suction blister model performed on 24 healthy volunteers.
28602514	5	15	theme	wound	937:941	arg1	healing					943:949	skin wound healing	932:949	skin wound healing	932:949	In parallel, we have examined the ability of JAD to promote growth factor activities and improve skin wound healing.
28602514	8	16	theme	JAD	1240:1242	arg1	activities					1226:1235	biological activities	1215:1235	biological activities of JAD	1215:1242	Finally, biological activities of JAD were assessed in cellulo, in FGF-7 induced migration/proliferation assays, as well as in vivo, using a suction blister model performed on 24 healthy volunteers.
28602514	11	17	theme	GAG	1718:1720	arg1	relationships					1741:1753	GAG structure-function relationships	1718:1753	GAG structure-function relationships	1718:1753	CONCLUSION This study highlights JAD as a promising compound for investigating GAG structure-function relationships and for applications in skin cosmetic /corrective strategies.
28602514	7	18	theme	GAG	1149:1151	arg1	composition					1153:1163	GAG composition	1149:1163	GAG composition	1149:1163	GAG composition was determined by disaccharide analysis.
28602514	5	19	theme	JAD	880:882	arg1	ability					869:875	the ability	865:875	the ability of JAD to promote growth factor activities and improve skin wound healing	865:949	In parallel, we have examined the ability of JAD to promote growth factor activities and improve skin wound healing.
28602514	1	20	theme	anti-cancer	183:193	arg1	properties					217:226	anti-cancer and anti-inflammatory properties	183:226	anti-cancer and anti-inflammatory properties	183:226	BACKGROUND Jasmonates are plant hormones that exhibit anti-cancer and anti-inflammatory properties and have therefore raised interest for human health applications.
28602514	0	21	theme	glycosaminoglycan	100:116	arg1	structure					118:126	glycosaminoglycan structure	100:126	glycosaminoglycan structure	100:126	A jasmonic acid derivative improves skin healing and induces changes in proteoglycan expression and glycosaminoglycan structure.
28602514	3	22	theme	extracellular	561:573	arg1	matrix					575:580	extracellular matrix	561:580	extracellular matrix (ECM) remodeling	561:597	Recently, we have reported that a jasmonate derivative (JAD) displayed anti-aging effects on human skin by inducing extracellular matrix (ECM) remodeling.
28602514	3	22	theme	extracellular	561:573	arg1	ECM					583:585	ECM	583:585	ECM	583:585	Recently, we have reported that a jasmonate derivative (JAD) displayed anti-aging effects on human skin by inducing extracellular matrix (ECM) remodeling.
28602514	4	23	theme	JAD	668:670	arg1	effects					657:663	here the effects	648:663	here the effects of JAD on proteoglycans and glycosaminoglycan (GAG) polysaccharides, which are major cell-surface/ECM components and are involved in a multitude of biological processes	648:832	Based on this observation, we have investigated here the effects of JAD on proteoglycans and glycosaminoglycan (GAG) polysaccharides, which are major cell-surface/ECM components and are involved in a multitude of biological processes.
28602514	0	24	theme	jasmonic	2:9	arg1	derivative					16:25	A jasmonic acid derivative	0:25	A jasmonic acid derivative	0:25	A jasmonic acid derivative improves skin healing and induces changes in proteoglycan expression and glycosaminoglycan structure.
28602514	11	25	from	applications	1763:1774	arg1	strategies					1805:1814	skin cosmetic /corrective strategies	1779:1814	skin cosmetic /corrective strategies	1779:1814	CONCLUSION This study highlights JAD as a promising compound for investigating GAG structure-function relationships and for applications in skin cosmetic /corrective strategies.
28602514	4	26	theme	cell-surface/ECM	750:765	arg1	components					767:776	major cell-surface/ECM components	744:776	major cell-surface/ECM components	744:776	Based on this observation, we have investigated here the effects of JAD on proteoglycans and glycosaminoglycan (GAG) polysaccharides, which are major cell-surface/ECM components and are involved in a multitude of biological processes.
28602514	4	26	theme	cell-surface/ECM	750:765	arg1	proteoglycans					675:687	proteoglycans	675:687	proteoglycans	675:687	Based on this observation, we have investigated here the effects of JAD on proteoglycans and glycosaminoglycan (GAG) polysaccharides, which are major cell-surface/ECM components and are involved in a multitude of biological processes.
28602514	4	26	theme	cell-surface/ECM	750:765	arg1	polysaccharides					717:731	glycosaminoglycan (GAG) polysaccharides	693:731	glycosaminoglycan (GAG) polysaccharides	693:731	Based on this observation, we have investigated here the effects of JAD on proteoglycans and glycosaminoglycan (GAG) polysaccharides, which are major cell-surface/ECM components and are involved in a multitude of biological processes.
28602514	1	27	theme	anti-inflammatory	199:215	arg1	properties					217:226	anti-cancer and anti-inflammatory properties	183:226	anti-cancer and anti-inflammatory properties	183:226	BACKGROUND Jasmonates are plant hormones that exhibit anti-cancer and anti-inflammatory properties and have therefore raised interest for human health applications.
28602514	12	28	theme	GENERAL	1817:1823	arg1	SIGNIFICANCE					1825:1836	GENERAL SIGNIFICANCE We	1817:1839	GENERAL SIGNIFICANCE We	1817:1839	GENERAL SIGNIFICANCE We propose here a novel mechanism, by which jasmonate derivatives may elicit biological activities in mammals.
28602514	4	29	theme	major	744:748	arg1	components					767:776	major cell-surface/ECM components	744:776	major cell-surface/ECM components	744:776	Based on this observation, we have investigated here the effects of JAD on proteoglycans and glycosaminoglycan (GAG) polysaccharides, which are major cell-surface/ECM components and are involved in a multitude of biological processes.
28602514	4	29	theme	major	744:748	arg1	proteoglycans					675:687	proteoglycans	675:687	proteoglycans	675:687	Based on this observation, we have investigated here the effects of JAD on proteoglycans and glycosaminoglycan (GAG) polysaccharides, which are major cell-surface/ECM components and are involved in a multitude of biological processes.
28602514	4	29	theme	major	744:748	arg1	polysaccharides					717:731	glycosaminoglycan (GAG) polysaccharides	693:731	glycosaminoglycan (GAG) polysaccharides	693:731	Based on this observation, we have investigated here the effects of JAD on proteoglycans and glycosaminoglycan (GAG) polysaccharides, which are major cell-surface/ECM components and are involved in a multitude of biological processes.
28602514	11	30	dep	CONCLUSION	1639:1648	arg1	highlights					1661:1670	highlights	1661:1670	highlights JAD as a promising compound for investigating GAG structure-function relationships and for applications in skin cosmetic /corrective strategies	1661:1814	CONCLUSION This study highlights JAD as a promising compound for investigating GAG structure-function relationships and for applications in skin cosmetic /corrective strategies.
28602514	2	31	theme	mechanisms	417:426	arg1	mechanisms					417:426	mechanisms	417:426	mechanisms	417:426	The molecular basis of these activities remains poorly understood, although increasing evidence suggests that a variety of mechanisms may be involved.
28602514	2	31	theme	mechanisms	417:426	arg1	variety					406:412	a variety	404:412	a variety of mechanisms	404:426	The molecular basis of these activities remains poorly understood, although increasing evidence suggests that a variety of mechanisms may be involved.
28602514	8	32	theme	suction	1347:1353	arg1	model					1363:1367	a suction blister model	1345:1367	a suction blister model performed on 24 healthy volunteers	1345:1402	Finally, biological activities of JAD were assessed in cellulo, in FGF-7 induced migration/proliferation assays, as well as in vivo, using a suction blister model performed on 24 healthy volunteers.
28602514	3	33	from	effects	527:533	arg1	skin					544:547	human skin	538:547	human skin	538:547	Recently, we have reported that a jasmonate derivative (JAD) displayed anti-aging effects on human skin by inducing extracellular matrix (ECM) remodeling.
28602514	6	34	theme	reconstituted	1036:1048	arg1	epidermis					1055:1063	reconstituted skin epidermis	1036:1063	reconstituted skin epidermis	1036:1063	METHODS Proteoglycan expression was analyzed on epidermal primary keratinocytes and reconstituted skin epidermis, using electron/immunofluorescence microscopy, western blotting and flow cytometry.
28602514	4	35	from	effects	657:663	arg1	components					767:776	major cell-surface/ECM components	744:776	major cell-surface/ECM components	744:776	Based on this observation, we have investigated here the effects of JAD on proteoglycans and glycosaminoglycan (GAG) polysaccharides, which are major cell-surface/ECM components and are involved in a multitude of biological processes.
28602514	4	35	from	effects	657:663	arg1	proteoglycans					675:687	proteoglycans	675:687	proteoglycans	675:687	Based on this observation, we have investigated here the effects of JAD on proteoglycans and glycosaminoglycan (GAG) polysaccharides, which are major cell-surface/ECM components and are involved in a multitude of biological processes.
28602514	4	35	from	effects	657:663	arg1	polysaccharides					717:731	glycosaminoglycan (GAG) polysaccharides	693:731	glycosaminoglycan (GAG) polysaccharides	693:731	Based on this observation, we have investigated here the effects of JAD on proteoglycans and glycosaminoglycan (GAG) polysaccharides, which are major cell-surface/ECM components and are involved in a multitude of biological processes.
28602514	0	36	theme	acid	11:14	arg1	derivative					16:25	A jasmonic acid derivative	0:25	A jasmonic acid derivative	0:25	A jasmonic acid derivative improves skin healing and induces changes in proteoglycan expression and glycosaminoglycan structure.
28602514	9	37	theme	GAG	1508:1510	arg1	structure					1512:1520	GAG structure	1508:1520	GAG structure	1508:1520	RESULTS JAD was found to induce expression of major skin proteoglycans and to induce subtle changes in GAG structure.
28602514	0	38	theme	skin	36:39	arg1	healing					41:47	skin healing	36:47	skin healing	36:47	A jasmonic acid derivative improves skin healing and induces changes in proteoglycan expression and glycosaminoglycan structure.
28602514	12	39	from	activities	1926:1935	arg1	mammals					1940:1946	mammals	1940:1946	mammals	1940:1946	GENERAL SIGNIFICANCE We propose here a novel mechanism, by which jasmonate derivatives may elicit biological activities in mammals.
28602514	6	40	theme	electron/immunofluorescence	1072:1098	arg1	microscopy					1100:1109	electron/immunofluorescence microscopy	1072:1109	electron/immunofluorescence microscopy	1072:1109	METHODS Proteoglycan expression was analyzed on epidermal primary keratinocytes and reconstituted skin epidermis, using electron/immunofluorescence microscopy, western blotting and flow cytometry.
28602514	6	41	theme	western	1112:1118	arg1	blotting					1120:1127	western blotting	1112:1127	western blotting	1112:1127	METHODS Proteoglycan expression was analyzed on epidermal primary keratinocytes and reconstituted skin epidermis, using electron/immunofluorescence microscopy, western blotting and flow cytometry.
28602514	3	42	theme	jasmonate	479:487	arg1	derivative					489:498	a jasmonate derivative	477:498	a jasmonate derivative (JAD)	477:504	Recently, we have reported that a jasmonate derivative (JAD) displayed anti-aging effects on human skin by inducing extracellular matrix (ECM) remodeling.
28602514	3	42	theme	jasmonate	479:487	arg1	JAD					501:503	JAD	501:503	JAD	501:503	Recently, we have reported that a jasmonate derivative (JAD) displayed anti-aging effects on human skin by inducing extracellular matrix (ECM) remodeling.
28602514	6	43	theme	flow	1133:1136	arg1	cytometry					1138:1146	flow cytometry	1133:1146	flow cytometry	1133:1146	METHODS Proteoglycan expression was analyzed on epidermal primary keratinocytes and reconstituted skin epidermis, using electron/immunofluorescence microscopy, western blotting and flow cytometry.
28602514	6	44	theme	Proteoglycan	960:971	arg1	expression					973:982	METHODS Proteoglycan expression	952:982	METHODS Proteoglycan expression	952:982	METHODS Proteoglycan expression was analyzed on epidermal primary keratinocytes and reconstituted skin epidermis, using electron/immunofluorescence microscopy, western blotting and flow cytometry.
28602514	0	45	from	changes	61:67	arg1	expression					85:94	proteoglycan expression	72:94	proteoglycan expression	72:94	A jasmonic acid derivative improves skin healing and induces changes in proteoglycan expression and glycosaminoglycan structure.
28602514	0	45	from	changes	61:67	arg1	structure					118:126	glycosaminoglycan structure	100:126	glycosaminoglycan structure	100:126	A jasmonic acid derivative improves skin healing and induces changes in proteoglycan expression and glycosaminoglycan structure.
28602514	6	46	theme	skin	1050:1053	arg1	epidermis					1055:1063	reconstituted skin epidermis	1036:1063	reconstituted skin epidermis	1036:1063	METHODS Proteoglycan expression was analyzed on epidermal primary keratinocytes and reconstituted skin epidermis, using electron/immunofluorescence microscopy, western blotting and flow cytometry.
28602514	8	47	theme	in	1330:1331	arg1	assays					1311:1316	FGF-7 induced migration/proliferation assays	1273:1316	FGF-7 induced migration/proliferation assays	1273:1316	Finally, biological activities of JAD were assessed in cellulo, in FGF-7 induced migration/proliferation assays, as well as in vivo, using a suction blister model performed on 24 healthy volunteers.
28602514	8	48	theme	induced	1279:1285	arg1	assays					1311:1316	FGF-7 induced migration/proliferation assays	1273:1316	FGF-7 induced migration/proliferation assays	1273:1316	Finally, biological activities of JAD were assessed in cellulo, in FGF-7 induced migration/proliferation assays, as well as in vivo, using a suction blister model performed on 24 healthy volunteers.
28602514	6	49	theme	METHODS	952:958	arg1	expression					973:982	METHODS Proteoglycan expression	952:982	METHODS Proteoglycan expression	952:982	METHODS Proteoglycan expression was analyzed on epidermal primary keratinocytes and reconstituted skin epidermis, using electron/immunofluorescence microscopy, western blotting and flow cytometry.
28602514	8	50	theme	healthy	1385:1391	arg1	volunteers					1393:1402	24 healthy volunteers	1382:1402	24 healthy volunteers	1382:1402	Finally, biological activities of JAD were assessed in cellulo, in FGF-7 induced migration/proliferation assays, as well as in vivo, using a suction blister model performed on 24 healthy volunteers.
28602514	10	51	theme	improved	1574:1581	arg1	healing					1588:1594	improved skin healing	1574:1594	improved skin healing	1574:1594	In parallel, we showed that JAD promoted FGF-7 and improved skin healing by accelerating epithelial repair in vivo.
28602514	11	52	theme	/corrective	1793:1803	arg1	strategies					1805:1814	skin cosmetic /corrective strategies	1779:1814	skin cosmetic /corrective strategies	1779:1814	CONCLUSION This study highlights JAD as a promising compound for investigating GAG structure-function relationships and for applications in skin cosmetic /corrective strategies.
28602514	3	53	theme	anti-aging	516:525	arg1	effects					527:533	anti-aging effects	516:533	anti-aging effects on human skin	516:547	Recently, we have reported that a jasmonate derivative (JAD) displayed anti-aging effects on human skin by inducing extracellular matrix (ECM) remodeling.
28602514	11	54	theme	skin	1779:1782	arg1	strategies					1805:1814	skin cosmetic /corrective strategies	1779:1814	skin cosmetic /corrective strategies	1779:1814	CONCLUSION This study highlights JAD as a promising compound for investigating GAG structure-function relationships and for applications in skin cosmetic /corrective strategies.
28602514	1	55	theme	BACKGROUND	129:138	arg1	Jasmonates					140:149	BACKGROUND Jasmonates	129:149	BACKGROUND Jasmonates	129:149	BACKGROUND Jasmonates are plant hormones that exhibit anti-cancer and anti-inflammatory properties and have therefore raised interest for human health applications.
28602514	1	55	theme	BACKGROUND	129:138	arg1	hormones					161:168	plant hormones	155:168	plant hormones that exhibit anti-cancer and anti-inflammatory properties and have therefore raised interest for human health applications	155:291	BACKGROUND Jasmonates are plant hormones that exhibit anti-cancer and anti-inflammatory properties and have therefore raised interest for human health applications.
28602514	11	56	theme	structure-function	1722:1739	arg1	relationships					1741:1753	GAG structure-function relationships	1718:1753	GAG structure-function relationships	1718:1753	CONCLUSION This study highlights JAD as a promising compound for investigating GAG structure-function relationships and for applications in skin cosmetic /corrective strategies.
28602514	11	57	theme	cosmetic	1784:1791	arg1	strategies					1805:1814	skin cosmetic /corrective strategies	1779:1814	skin cosmetic /corrective strategies	1779:1814	CONCLUSION This study highlights JAD as a promising compound for investigating GAG structure-function relationships and for applications in skin cosmetic /corrective strategies.
28602514	2	58	theme	molecular	298:306	arg1	basis					308:312	The molecular basis	294:312	The molecular basis of these activities	294:332	The molecular basis of these activities remains poorly understood, although increasing evidence suggests that a variety of mechanisms may be involved.
28602514	12	59	theme	jasmonate	1882:1890	arg1	derivatives					1892:1902	jasmonate derivatives	1882:1902	jasmonate derivatives	1882:1902	GENERAL SIGNIFICANCE We propose here a novel mechanism, by which jasmonate derivatives may elicit biological activities in mammals.
28602514	2	60	theme	increasing	370:379	arg1	evidence					381:388	increasing evidence	370:388	increasing evidence	370:388	The molecular basis of these activities remains poorly understood, although increasing evidence suggests that a variety of mechanisms may be involved.
28602514	12	61	theme	biological	1915:1924	arg1	activities					1926:1935	biological activities	1915:1935	biological activities in mammals	1915:1946	GENERAL SIGNIFICANCE We propose here a novel mechanism, by which jasmonate derivatives may elicit biological activities in mammals.
28602514	2	62	theme	activities	323:332	arg1	basis					308:312	The molecular basis	294:312	The molecular basis of these activities	294:332	The molecular basis of these activities remains poorly understood, although increasing evidence suggests that a variety of mechanisms may be involved.
28602514	12	63	theme	novel	1856:1860	arg1	mechanism					1862:1870	a novel mechanism	1854:1870	a novel mechanism	1854:1870	GENERAL SIGNIFICANCE We propose here a novel mechanism, by which jasmonate derivatives may elicit biological activities in mammals.
28602514	5	64	theme	growth	895:900	arg1	activities					909:918	growth factor activities	895:918	growth factor activities	895:918	In parallel, we have examined the ability of JAD to promote growth factor activities and improve skin wound healing.
28602514	9	65	theme	RESULTS	1405:1411	arg1	JAD					1413:1415	RESULTS JAD	1405:1415	RESULTS JAD	1405:1415	RESULTS JAD was found to induce expression of major skin proteoglycans and to induce subtle changes in GAG structure.
28602514	5	66	theme	skin	932:935	arg1	healing					943:949	skin wound healing	932:949	skin wound healing	932:949	In parallel, we have examined the ability of JAD to promote growth factor activities and improve skin wound healing.
28602514	10	67	theme	skin	1583:1586	arg1	healing					1588:1594	improved skin healing	1574:1594	improved skin healing	1574:1594	In parallel, we showed that JAD promoted FGF-7 and improved skin healing by accelerating epithelial repair in vivo.
28602514	4	68	theme	processes	824:832	arg1	multitude					800:808	a multitude	798:808	a multitude of biological processes	798:832	Based on this observation, we have investigated here the effects of JAD on proteoglycans and glycosaminoglycan (GAG) polysaccharides, which are major cell-surface/ECM components and are involved in a multitude of biological processes.
28602514	9	69	from	changes	1497:1503	arg1	structure					1512:1520	GAG structure	1508:1520	GAG structure	1508:1520	RESULTS JAD was found to induce expression of major skin proteoglycans and to induce subtle changes in GAG structure.
28602514	1	70	theme	plant	155:159	arg1	Jasmonates					140:149	BACKGROUND Jasmonates	129:149	BACKGROUND Jasmonates	129:149	BACKGROUND Jasmonates are plant hormones that exhibit anti-cancer and anti-inflammatory properties and have therefore raised interest for human health applications.
28602514	1	70	theme	plant	155:159	arg1	hormones					161:168	plant hormones	155:168	plant hormones that exhibit anti-cancer and anti-inflammatory properties and have therefore raised interest for human health applications	155:291	BACKGROUND Jasmonates are plant hormones that exhibit anti-cancer and anti-inflammatory properties and have therefore raised interest for human health applications.
28602514	8	71	theme	migration/proliferation	1287:1309	arg1	assays					1311:1316	FGF-7 induced migration/proliferation assays	1273:1316	FGF-7 induced migration/proliferation assays	1273:1316	Finally, biological activities of JAD were assessed in cellulo, in FGF-7 induced migration/proliferation assays, as well as in vivo, using a suction blister model performed on 24 healthy volunteers.
28602514	1	72	theme	human	267:271	arg1	applications					280:291	human health applications	267:291	human health applications	267:291	BACKGROUND Jasmonates are plant hormones that exhibit anti-cancer and anti-inflammatory properties and have therefore raised interest for human health applications.
28602514	9	73	theme	proteoglycans	1462:1474	arg1	expression					1437:1446	expression	1437:1446	expression of major skin proteoglycans	1437:1474	RESULTS JAD was found to induce expression of major skin proteoglycans and to induce subtle changes in GAG structure.
28602514	9	74	theme	subtle	1490:1495	arg1	changes					1497:1503	subtle changes	1490:1503	subtle changes in GAG structure	1490:1520	RESULTS JAD was found to induce expression of major skin proteoglycans and to induce subtle changes in GAG structure.
28602514	4	75	theme	biological	813:822	arg1	processes					824:832	biological processes	813:832	biological processes	813:832	Based on this observation, we have investigated here the effects of JAD on proteoglycans and glycosaminoglycan (GAG) polysaccharides, which are major cell-surface/ECM components and are involved in a multitude of biological processes.
28602514	4	76	theme	glycosaminoglycan	693:709	arg1	components					767:776	major cell-surface/ECM components	744:776	major cell-surface/ECM components	744:776	Based on this observation, we have investigated here the effects of JAD on proteoglycans and glycosaminoglycan (GAG) polysaccharides, which are major cell-surface/ECM components and are involved in a multitude of biological processes.
28602514	4	76	theme	glycosaminoglycan	693:709	arg1	proteoglycans					675:687	proteoglycans	675:687	proteoglycans	675:687	Based on this observation, we have investigated here the effects of JAD on proteoglycans and glycosaminoglycan (GAG) polysaccharides, which are major cell-surface/ECM components and are involved in a multitude of biological processes.
28602514	4	76	theme	glycosaminoglycan	693:709	arg1	polysaccharides					717:731	glycosaminoglycan (GAG) polysaccharides	693:731	glycosaminoglycan (GAG) polysaccharides	693:731	Based on this observation, we have investigated here the effects of JAD on proteoglycans and glycosaminoglycan (GAG) polysaccharides, which are major cell-surface/ECM components and are involved in a multitude of biological processes.
28602514	1	77	theme	health	273:278	arg1	applications					280:291	human health applications	267:291	human health applications	267:291	BACKGROUND Jasmonates are plant hormones that exhibit anti-cancer and anti-inflammatory properties and have therefore raised interest for human health applications.
24751262	5	0	theme	fibers	645:650	arg1	strength					601:608	strength	601:608	strength	601:608	% of chitin nanofibrils into chitosan matrix led to an increase in strength and Young modulus of the composite fibers.
24751262	5	0	theme	fibers	645:650	arg1	modulus					620:626	Young modulus	614:626	Young modulus	614:626	% of chitin nanofibrils into chitosan matrix led to an increase in strength and Young modulus of the composite fibers.
24751262	5	1	theme	chitin	539:544	arg1	nanofibrils					546:556	chitin nanofibrils	539:556	chitin nanofibrils	539:556	% of chitin nanofibrils into chitosan matrix led to an increase in strength and Young modulus of the composite fibers.
24751262	1	2	theme	chitin	160:165	arg1	nanofibrils					167:177	anisotropic chitin nanofibrils	148:177	anisotropic chitin nanofibrils	148:177	Biocompatible and bioresorbable composite fibers consisting of chitosan filled with anisotropic chitin nanofibrils with the length of 600-800 nm and cross section of about 11-12 nm as revealed by SEM and XRD were prepared by coagulation.
24751262	1	3	theme	nm	242:243	arg1	length					188:193	the length	184:193	the length of 600-800 nm	184:207	Biocompatible and bioresorbable composite fibers consisting of chitosan filled with anisotropic chitin nanofibrils with the length of 600-800 nm and cross section of about 11-12 nm as revealed by SEM and XRD were prepared by coagulation.
24751262	1	3	theme	nm	242:243	arg1	section					219:225	cross section	213:225	cross section of about 11-12 nm	213:243	Biocompatible and bioresorbable composite fibers consisting of chitosan filled with anisotropic chitin nanofibrils with the length of 600-800 nm and cross section of about 11-12 nm as revealed by SEM and XRD were prepared by coagulation.
24751262	4	4	theme	wt	531:532	arg1	incorporation					506:518	The incorporation	502:518	The incorporation of 0.1-0.3 wt	502:532	The incorporation of 0.1-0.3 wt.
24751262	5	5	theme	Young	614:618	arg1	modulus					620:626	Young modulus	614:626	Young modulus	614:626	% of chitin nanofibrils into chitosan matrix led to an increase in strength and Young modulus of the composite fibers.
24751262	1	6	theme	Biocompatible	64:76	arg1	fibers					106:111	Biocompatible and bioresorbable composite fibers	64:111	Biocompatible and bioresorbable composite fibers consisting of chitosan filled with anisotropic chitin nanofibrils with the length of 600-800 nm and cross section of about 11-12 nm as revealed by SEM and XRD	64:270	Biocompatible and bioresorbable composite fibers consisting of chitosan filled with anisotropic chitin nanofibrils with the length of 600-800 nm and cross section of about 11-12 nm as revealed by SEM and XRD were prepared by coagulation.
24751262	2	7	theme	preferred	372:380	arg1	orientations					382:393	preferred orientations	372:393	preferred orientations	372:393	Both chitin and chitosan components of the composite fibers displayed preferred orientations.
24751262	2	8	dep	chitin	307:312	arg1	components					327:336	components	327:336	components	327:336	Both chitin and chitosan components of the composite fibers displayed preferred orientations.
24751262	3	9	theme	molecules	420:428	arg1	Orientation					396:406	Orientation	396:406	Orientation of chitosan molecules induced by chitin nanocrystallites	396:463	Orientation of chitosan molecules induced by chitin nanocrystallites was confirmed by molecular modeling.
24751262	0	10	theme	Wet	0:2	arg1	spinning					4:11	Wet spinning	0:11	Wet spinning of fibers	0:21	Wet spinning of fibers made of chitosan and chitin nanofibrils.
24751262	5	11	theme	composite	635:643	arg1	fibers					645:650	the composite fibers	631:650	the composite fibers	631:650	% of chitin nanofibrils into chitosan matrix led to an increase in strength and Young modulus of the composite fibers.
24751262	1	12	theme	bioresorbable	82:94	arg1	fibers					106:111	Biocompatible and bioresorbable composite fibers	64:111	Biocompatible and bioresorbable composite fibers consisting of chitosan filled with anisotropic chitin nanofibrils with the length of 600-800 nm and cross section of about 11-12 nm as revealed by SEM and XRD	64:270	Biocompatible and bioresorbable composite fibers consisting of chitosan filled with anisotropic chitin nanofibrils with the length of 600-800 nm and cross section of about 11-12 nm as revealed by SEM and XRD were prepared by coagulation.
24751262	2	13	theme	fibers	355:360	arg1	chitin					307:312	chitin	307:312	chitin	307:312	Both chitin and chitosan components of the composite fibers displayed preferred orientations.
24751262	0	14	theme	fibers	16:21	arg1	spinning					4:11	Wet spinning	0:11	Wet spinning of fibers	0:21	Wet spinning of fibers made of chitosan and chitin nanofibrils.
24751262	1	15	theme	composite	96:104	arg1	fibers					106:111	Biocompatible and bioresorbable composite fibers	64:111	Biocompatible and bioresorbable composite fibers consisting of chitosan filled with anisotropic chitin nanofibrils with the length of 600-800 nm and cross section of about 11-12 nm as revealed by SEM and XRD	64:270	Biocompatible and bioresorbable composite fibers consisting of chitosan filled with anisotropic chitin nanofibrils with the length of 600-800 nm and cross section of about 11-12 nm as revealed by SEM and XRD were prepared by coagulation.
24751262	1	16	theme	nm	206:207	arg1	length					188:193	the length	184:193	the length of 600-800 nm	184:207	Biocompatible and bioresorbable composite fibers consisting of chitosan filled with anisotropic chitin nanofibrils with the length of 600-800 nm and cross section of about 11-12 nm as revealed by SEM and XRD were prepared by coagulation.
24751262	1	16	theme	nm	206:207	arg1	section					219:225	cross section	213:225	cross section of about 11-12 nm	213:243	Biocompatible and bioresorbable composite fibers consisting of chitosan filled with anisotropic chitin nanofibrils with the length of 600-800 nm and cross section of about 11-12 nm as revealed by SEM and XRD were prepared by coagulation.
24751262	3	17	theme	chitosan	411:418	arg1	molecules					420:428	chitosan molecules	411:428	chitosan molecules induced by chitin nanocrystallites	411:463	Orientation of chitosan molecules induced by chitin nanocrystallites was confirmed by molecular modeling.
24751262	0	18	dep	chitosan	31:38	arg1	nanofibrils					51:61	nanofibrils	51:61	nanofibrils	51:61	Wet spinning of fibers made of chitosan and chitin nanofibrils.
24751262	5	19	theme	nanofibrils	546:556	arg1	%					534:534	%	534:534	% of chitin nanofibrils into chitosan matrix	534:577	% of chitin nanofibrils into chitosan matrix led to an increase in strength and Young modulus of the composite fibers.
24751262	5	19	theme	nanofibrils	546:556	arg1	nanofibrils					546:556	chitin nanofibrils	539:556	chitin nanofibrils	539:556	% of chitin nanofibrils into chitosan matrix led to an increase in strength and Young modulus of the composite fibers.
24751262	1	20	theme	cross	213:217	arg1	section					219:225	cross section	213:225	cross section of about 11-12 nm	213:243	Biocompatible and bioresorbable composite fibers consisting of chitosan filled with anisotropic chitin nanofibrils with the length of 600-800 nm and cross section of about 11-12 nm as revealed by SEM and XRD were prepared by coagulation.
24751262	5	21	theme	chitosan	563:570	arg1	matrix					572:577	chitosan matrix	563:577	chitosan matrix	563:577	% of chitin nanofibrils into chitosan matrix led to an increase in strength and Young modulus of the composite fibers.
24751262	5	22	from	increase	589:596	arg1	strength					601:608	strength	601:608	strength	601:608	% of chitin nanofibrils into chitosan matrix led to an increase in strength and Young modulus of the composite fibers.
24751262	5	22	from	increase	589:596	arg1	modulus					620:626	Young modulus	614:626	Young modulus	614:626	% of chitin nanofibrils into chitosan matrix led to an increase in strength and Young modulus of the composite fibers.
24751262	3	23	theme	molecular	482:490	arg1	modeling					492:499	molecular modeling	482:499	molecular modeling	482:499	Orientation of chitosan molecules induced by chitin nanocrystallites was confirmed by molecular modeling.
24751262	2	24	theme	composite	345:353	arg1	fibers					355:360	the composite fibers	341:360	the composite fibers	341:360	Both chitin and chitosan components of the composite fibers displayed preferred orientations.
24751262	3	25	theme	chitin	441:446	arg1	nanocrystallites					448:463	chitin nanocrystallites	441:463	chitin nanocrystallites	441:463	Orientation of chitosan molecules induced by chitin nanocrystallites was confirmed by molecular modeling.
24751262	1	26	theme	anisotropic	148:158	arg1	nanofibrils					167:177	anisotropic chitin nanofibrils	148:177	anisotropic chitin nanofibrils	148:177	Biocompatible and bioresorbable composite fibers consisting of chitosan filled with anisotropic chitin nanofibrils with the length of 600-800 nm and cross section of about 11-12 nm as revealed by SEM and XRD were prepared by coagulation.
27746354	0	0	theme	microspheres	94:105	arg1	characteristic					16:29	characteristic	16:29	characteristic	16:29	Preparation and characteristic of gelatine/oxidized corn starch and gelatin/corn starch blend microspheres.
27746354	0	0	theme	microspheres	94:105	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characteristic of gelatine/oxidized corn starch and gelatin/corn starch blend microspheres.
27746354	3	1	theme	single	549:554	arg1	technique					571:579	the single droplet drying technique	545:579	the single droplet drying technique	545:579	The drying and dissolution behaviors of composite droplet have been studied using the single droplet drying technique.
27746354	2	2	theme	wt	458:459	arg1	%					457:457	1%wt; 3%wt; 5%wt	444:459	1%wt; 3%wt; 5%wt	444:459	The blending solutions property, gel time, transparency and viscosity of G/CS (corn starch) and G/OCS blend solutions were compared at different ratios (10:0;9:1;8:2;7:3;6:4;5:5) and concentrations(1%wt; 3%wt; 5%wt).
27746354	2	2	theme	wt	458:459	arg1	concentrations					429:442	concentrations	429:442	concentrations(1%wt; 3%wt; 5%wt)	429:460	The blending solutions property, gel time, transparency and viscosity of G/CS (corn starch) and G/OCS blend solutions were compared at different ratios (10:0;9:1;8:2;7:3;6:4;5:5) and concentrations(1%wt; 3%wt; 5%wt).
27746354	1	3	theme	starch	154:159	arg1	Combinations					108:119	Combinations	108:119	Combinations of gelatin (G) and oxidized corn starch (OCS)	108:165	Combinations of gelatin (G) and oxidized corn starch (OCS) were explored as a new microcapsule composite for single droplet spray drying.
27746354	1	3	theme	starch	154:159	arg1	composite					203:211	a new microcapsule composite	184:211	a new microcapsule composite for single droplet spray drying	184:243	Combinations of gelatin (G) and oxidized corn starch (OCS) were explored as a new microcapsule composite for single droplet spray drying.
27746354	1	4	theme	spray	232:236	arg1	drying					238:243	single droplet spray drying	217:243	single droplet spray drying	217:243	Combinations of gelatin (G) and oxidized corn starch (OCS) were explored as a new microcapsule composite for single droplet spray drying.
27746354	0	5	theme	blend	88:92	arg1	microspheres					94:105	gelatin/corn starch blend microspheres	68:105	gelatin/corn starch blend microspheres	68:105	Preparation and characteristic of gelatine/oxidized corn starch and gelatin/corn starch blend microspheres.
27746354	5	6	theme	lower	752:756	arg1	viscosity					758:766	lower viscosity	752:766	lower viscosity	752:766	Blends solutions of G/OCS showed longer Gel time, higher transparency and lower viscosity; further displayed faster dissolution rate than that of G/CS under similar conditions.
27746354	4	7	theme	composite	618:626	arg1	blend					628:632	the composite blend	614:632	the composite blend	614:632	Possible reaction mechanisms in the composite blend were elucidated by SEM and FTIR techniques.
27746354	2	8	theme	gel	279:281	arg1	time					283:286	gel time	279:286	gel time	279:286	The blending solutions property, gel time, transparency and viscosity of G/CS (corn starch) and G/OCS blend solutions were compared at different ratios (10:0;9:1;8:2;7:3;6:4;5:5) and concentrations(1%wt; 3%wt; 5%wt).
27746354	2	8	theme	gel	279:281	arg1	solutions					259:267	The blending solutions	246:267	The blending solutions property, gel time, transparency and viscosity of G/CS (corn starch) and G/OCS blend solutions	246:362	The blending solutions property, gel time, transparency and viscosity of G/CS (corn starch) and G/OCS blend solutions were compared at different ratios (10:0;9:1;8:2;7:3;6:4;5:5) and concentrations(1%wt; 3%wt; 5%wt).
27746354	5	9	theme	faster	787:792	arg1	rate					806:809	faster dissolution rate	787:809	faster dissolution rate than that of G/CS	787:827	Blends solutions of G/OCS showed longer Gel time, higher transparency and lower viscosity; further displayed faster dissolution rate than that of G/CS under similar conditions.
27746354	7	10	theme	excellent	1073:1081	arg1	properties					1083:1092	excellent properties	1073:1092	excellent properties	1073:1092	All results indicated that the composites could be prepared with excellent properties by G/OCS (6:4) which would overcome some disadvantage such as thermodynamic incompatibility and phase separation by G/CS.
27746354	2	11	theme	corn	325:328	arg1	G/CS					319:322	G/CS	319:322	G/CS (corn starch)	319:336	The blending solutions property, gel time, transparency and viscosity of G/CS (corn starch) and G/OCS blend solutions were compared at different ratios (10:0;9:1;8:2;7:3;6:4;5:5) and concentrations(1%wt; 3%wt; 5%wt).
27746354	2	11	theme	corn	325:328	arg1	starch					330:335	corn starch	325:335	corn starch	325:335	The blending solutions property, gel time, transparency and viscosity of G/CS (corn starch) and G/OCS blend solutions were compared at different ratios (10:0;9:1;8:2;7:3;6:4;5:5) and concentrations(1%wt; 3%wt; 5%wt).
27746354	5	12	theme	longer	711:716	arg1	time					722:725	longer Gel time	711:725	longer Gel time	711:725	Blends solutions of G/OCS showed longer Gel time, higher transparency and lower viscosity; further displayed faster dissolution rate than that of G/CS under similar conditions.
27746354	6	13	theme	aldehyde	913:920	arg1	G					954:954	G	954:954	G which improved the compatibility between G and OCS	954:1005	This was attributed to the formed Schiff base between the aldehyde group of OCS and amino group of G which improved the compatibility between G and OCS.
27746354	6	13	theme	aldehyde	913:920	arg1	OCS					931:933	OCS	931:933	OCS	931:933	This was attributed to the formed Schiff base between the aldehyde group of OCS and amino group of G which improved the compatibility between G and OCS.
27746354	6	13	theme	aldehyde	913:920	arg1	group					922:926	the aldehyde group	909:926	the aldehyde group of OCS	909:933	This was attributed to the formed Schiff base between the aldehyde group of OCS and amino group of G which improved the compatibility between G and OCS.
27746354	2	14	theme	solutions	354:362	arg1	property					269:276	property	269:276	property	269:276	The blending solutions property, gel time, transparency and viscosity of G/CS (corn starch) and G/OCS blend solutions were compared at different ratios (10:0;9:1;8:2;7:3;6:4;5:5) and concentrations(1%wt; 3%wt; 5%wt).
27746354	2	14	theme	solutions	354:362	arg1	time					283:286	gel time	279:286	gel time	279:286	The blending solutions property, gel time, transparency and viscosity of G/CS (corn starch) and G/OCS blend solutions were compared at different ratios (10:0;9:1;8:2;7:3;6:4;5:5) and concentrations(1%wt; 3%wt; 5%wt).
27746354	2	14	theme	solutions	354:362	arg1	viscosity					306:314	viscosity	306:314	viscosity	306:314	The blending solutions property, gel time, transparency and viscosity of G/CS (corn starch) and G/OCS blend solutions were compared at different ratios (10:0;9:1;8:2;7:3;6:4;5:5) and concentrations(1%wt; 3%wt; 5%wt).
27746354	2	14	theme	solutions	354:362	arg1	solutions					259:267	The blending solutions	246:267	The blending solutions property, gel time, transparency and viscosity of G/CS (corn starch) and G/OCS blend solutions	246:362	The blending solutions property, gel time, transparency and viscosity of G/CS (corn starch) and G/OCS blend solutions were compared at different ratios (10:0;9:1;8:2;7:3;6:4;5:5) and concentrations(1%wt; 3%wt; 5%wt).
27746354	2	14	theme	solutions	354:362	arg1	transparency					289:300	transparency	289:300	transparency	289:300	The blending solutions property, gel time, transparency and viscosity of G/CS (corn starch) and G/OCS blend solutions were compared at different ratios (10:0;9:1;8:2;7:3;6:4;5:5) and concentrations(1%wt; 3%wt; 5%wt).
27746354	6	15	theme	formed	882:887	arg1	base					896:899	the formed Schiff base	878:899	the formed Schiff base between the aldehyde group of OCS and amino group of G which improved the compatibility between G and OCS	878:1005	This was attributed to the formed Schiff base between the aldehyde group of OCS and amino group of G which improved the compatibility between G and OCS.
27746354	3	16	theme	droplet	556:562	arg1	technique					571:579	the single droplet drying technique	545:579	the single droplet drying technique	545:579	The drying and dissolution behaviors of composite droplet have been studied using the single droplet drying technique.
27746354	2	17	dep	ratios	391:396	arg1	10:0					399:402	10:0	399:402	10:0;9:1;8:2;7:3;6:4;5:5	399:422	The blending solutions property, gel time, transparency and viscosity of G/CS (corn starch) and G/OCS blend solutions were compared at different ratios (10:0;9:1;8:2;7:3;6:4;5:5) and concentrations(1%wt; 3%wt; 5%wt).
27746354	2	18	theme	blending	250:257	arg1	property					269:276	property	269:276	property	269:276	The blending solutions property, gel time, transparency and viscosity of G/CS (corn starch) and G/OCS blend solutions were compared at different ratios (10:0;9:1;8:2;7:3;6:4;5:5) and concentrations(1%wt; 3%wt; 5%wt).
27746354	2	18	theme	blending	250:257	arg1	time					283:286	gel time	279:286	gel time	279:286	The blending solutions property, gel time, transparency and viscosity of G/CS (corn starch) and G/OCS blend solutions were compared at different ratios (10:0;9:1;8:2;7:3;6:4;5:5) and concentrations(1%wt; 3%wt; 5%wt).
27746354	2	18	theme	blending	250:257	arg1	viscosity					306:314	viscosity	306:314	viscosity	306:314	The blending solutions property, gel time, transparency and viscosity of G/CS (corn starch) and G/OCS blend solutions were compared at different ratios (10:0;9:1;8:2;7:3;6:4;5:5) and concentrations(1%wt; 3%wt; 5%wt).
27746354	2	18	theme	blending	250:257	arg1	solutions					259:267	The blending solutions	246:267	The blending solutions property, gel time, transparency and viscosity of G/CS (corn starch) and G/OCS blend solutions	246:362	The blending solutions property, gel time, transparency and viscosity of G/CS (corn starch) and G/OCS blend solutions were compared at different ratios (10:0;9:1;8:2;7:3;6:4;5:5) and concentrations(1%wt; 3%wt; 5%wt).
27746354	2	18	theme	blending	250:257	arg1	transparency					289:300	transparency	289:300	transparency	289:300	The blending solutions property, gel time, transparency and viscosity of G/CS (corn starch) and G/OCS blend solutions were compared at different ratios (10:0;9:1;8:2;7:3;6:4;5:5) and concentrations(1%wt; 3%wt; 5%wt).
27746354	6	19	theme	OCS	931:933	arg1	G					954:954	G	954:954	G which improved the compatibility between G and OCS	954:1005	This was attributed to the formed Schiff base between the aldehyde group of OCS and amino group of G which improved the compatibility between G and OCS.
27746354	6	19	theme	OCS	931:933	arg1	OCS					931:933	OCS	931:933	OCS	931:933	This was attributed to the formed Schiff base between the aldehyde group of OCS and amino group of G which improved the compatibility between G and OCS.
27746354	6	19	theme	OCS	931:933	arg1	group					945:949	amino group	939:949	amino group of G which improved the compatibility between G and OCS	939:1005	This was attributed to the formed Schiff base between the aldehyde group of OCS and amino group of G which improved the compatibility between G and OCS.
27746354	6	19	theme	OCS	931:933	arg1	group					922:926	the aldehyde group	909:926	the aldehyde group of OCS	909:933	This was attributed to the formed Schiff base between the aldehyde group of OCS and amino group of G which improved the compatibility between G and OCS.
27746354	2	20	theme	blend	348:352	arg1	solutions					354:362	G/OCS blend solutions	342:362	G/OCS blend solutions	342:362	The blending solutions property, gel time, transparency and viscosity of G/CS (corn starch) and G/OCS blend solutions were compared at different ratios (10:0;9:1;8:2;7:3;6:4;5:5) and concentrations(1%wt; 3%wt; 5%wt).
27746354	3	21	theme	drying	564:569	arg1	technique					571:579	the single droplet drying technique	545:579	the single droplet drying technique	545:579	The drying and dissolution behaviors of composite droplet have been studied using the single droplet drying technique.
27746354	4	22	from	mechanisms	600:609	arg1	blend					628:632	the composite blend	614:632	the composite blend	614:632	Possible reaction mechanisms in the composite blend were elucidated by SEM and FTIR techniques.
27746354	4	23	theme	reaction	591:598	arg1	mechanisms					600:609	Possible reaction mechanisms	582:609	Possible reaction mechanisms in the composite blend	582:632	Possible reaction mechanisms in the composite blend were elucidated by SEM and FTIR techniques.
27746354	2	24	theme	G/OCS	342:346	arg1	solutions					354:362	G/OCS blend solutions	342:362	G/OCS blend solutions	342:362	The blending solutions property, gel time, transparency and viscosity of G/CS (corn starch) and G/OCS blend solutions were compared at different ratios (10:0;9:1;8:2;7:3;6:4;5:5) and concentrations(1%wt; 3%wt; 5%wt).
27746354	2	25	dep	10:0	399:402	arg1	6:4					416:418	6:4	416:418	6:4	416:418	The blending solutions property, gel time, transparency and viscosity of G/CS (corn starch) and G/OCS blend solutions were compared at different ratios (10:0;9:1;8:2;7:3;6:4;5:5) and concentrations(1%wt; 3%wt; 5%wt).
27746354	2	25	dep	10:0	399:402	arg1	7:3					412:414	7:3	412:414	10:0;9:1;8:2;7:3;6:4;5:5	399:422	The blending solutions property, gel time, transparency and viscosity of G/CS (corn starch) and G/OCS blend solutions were compared at different ratios (10:0;9:1;8:2;7:3;6:4;5:5) and concentrations(1%wt; 3%wt; 5%wt).
27746354	2	25	dep	10:0	399:402	arg1	8:2					408:410	8:2	408:410	10:0;9:1;8:2;7:3;6:4;5:5	399:422	The blending solutions property, gel time, transparency and viscosity of G/CS (corn starch) and G/OCS blend solutions were compared at different ratios (10:0;9:1;8:2;7:3;6:4;5:5) and concentrations(1%wt; 3%wt; 5%wt).
27746354	2	25	dep	10:0	399:402	arg1	9:1					404:406	9:1	404:406	10:0;9:1;8:2;7:3;6:4;5:5	399:422	The blending solutions property, gel time, transparency and viscosity of G/CS (corn starch) and G/OCS blend solutions were compared at different ratios (10:0;9:1;8:2;7:3;6:4;5:5) and concentrations(1%wt; 3%wt; 5%wt).
27746354	2	25	dep	10:0	399:402	arg1	5:5					420:422	5:5	420:422	5:5	420:422	The blending solutions property, gel time, transparency and viscosity of G/CS (corn starch) and G/OCS blend solutions were compared at different ratios (10:0;9:1;8:2;7:3;6:4;5:5) and concentrations(1%wt; 3%wt; 5%wt).
27746354	4	26	theme	Possible	582:589	arg1	mechanisms					600:609	Possible reaction mechanisms	582:609	Possible reaction mechanisms in the composite blend	582:632	Possible reaction mechanisms in the composite blend were elucidated by SEM and FTIR techniques.
27746354	2	27	theme	wt	446:447	arg1	%					457:457	1%wt; 3%wt; 5%wt	444:459	1%wt; 3%wt; 5%wt	444:459	The blending solutions property, gel time, transparency and viscosity of G/CS (corn starch) and G/OCS blend solutions were compared at different ratios (10:0;9:1;8:2;7:3;6:4;5:5) and concentrations(1%wt; 3%wt; 5%wt).
27746354	2	27	theme	wt	446:447	arg1	concentrations					429:442	concentrations	429:442	concentrations(1%wt; 3%wt; 5%wt)	429:460	The blending solutions property, gel time, transparency and viscosity of G/CS (corn starch) and G/OCS blend solutions were compared at different ratios (10:0;9:1;8:2;7:3;6:4;5:5) and concentrations(1%wt; 3%wt; 5%wt).
27746354	6	28	theme	Schiff	889:894	arg1	base					896:899	the formed Schiff base	878:899	the formed Schiff base between the aldehyde group of OCS and amino group of G which improved the compatibility between G and OCS	878:1005	This was attributed to the formed Schiff base between the aldehyde group of OCS and amino group of G which improved the compatibility between G and OCS.
27746354	2	29	theme	different	381:389	arg1	ratios					391:396	different ratios	381:396	different ratios (10:0;9:1;8:2;7:3;6:4;5:5)	381:423	The blending solutions property, gel time, transparency and viscosity of G/CS (corn starch) and G/OCS blend solutions were compared at different ratios (10:0;9:1;8:2;7:3;6:4;5:5) and concentrations(1%wt; 3%wt; 5%wt).
27746354	5	30	theme	dissolution	794:804	arg1	rate					806:809	faster dissolution rate	787:809	faster dissolution rate than that of G/CS	787:827	Blends solutions of G/OCS showed longer Gel time, higher transparency and lower viscosity; further displayed faster dissolution rate than that of G/CS under similar conditions.
27746354	2	31	theme	%	445:445	arg1	%					457:457	1%wt; 3%wt; 5%wt	444:459	1%wt; 3%wt; 5%wt	444:459	The blending solutions property, gel time, transparency and viscosity of G/CS (corn starch) and G/OCS blend solutions were compared at different ratios (10:0;9:1;8:2;7:3;6:4;5:5) and concentrations(1%wt; 3%wt; 5%wt).
27746354	2	31	theme	%	445:445	arg1	concentrations					429:442	concentrations	429:442	concentrations(1%wt; 3%wt; 5%wt)	429:460	The blending solutions property, gel time, transparency and viscosity of G/CS (corn starch) and G/OCS blend solutions were compared at different ratios (10:0;9:1;8:2;7:3;6:4;5:5) and concentrations(1%wt; 3%wt; 5%wt).
27746354	1	32	theme	gelatin	124:130	arg1	Combinations					108:119	Combinations	108:119	Combinations of gelatin (G) and oxidized corn starch (OCS)	108:165	Combinations of gelatin (G) and oxidized corn starch (OCS) were explored as a new microcapsule composite for single droplet spray drying.
27746354	1	32	theme	gelatin	124:130	arg1	composite					203:211	a new microcapsule composite	184:211	a new microcapsule composite for single droplet spray drying	184:243	Combinations of gelatin (G) and oxidized corn starch (OCS) were explored as a new microcapsule composite for single droplet spray drying.
27746354	2	33	dep	solutions	259:267	arg1	property					269:276	property	269:276	property	269:276	The blending solutions property, gel time, transparency and viscosity of G/CS (corn starch) and G/OCS blend solutions were compared at different ratios (10:0;9:1;8:2;7:3;6:4;5:5) and concentrations(1%wt; 3%wt; 5%wt).
27746354	2	33	dep	solutions	259:267	arg1	time					283:286	gel time	279:286	gel time	279:286	The blending solutions property, gel time, transparency and viscosity of G/CS (corn starch) and G/OCS blend solutions were compared at different ratios (10:0;9:1;8:2;7:3;6:4;5:5) and concentrations(1%wt; 3%wt; 5%wt).
27746354	2	33	dep	solutions	259:267	arg1	solutions					259:267	The blending solutions	246:267	The blending solutions property, gel time, transparency and viscosity of G/CS (corn starch) and G/OCS blend solutions	246:362	The blending solutions property, gel time, transparency and viscosity of G/CS (corn starch) and G/OCS blend solutions were compared at different ratios (10:0;9:1;8:2;7:3;6:4;5:5) and concentrations(1%wt; 3%wt; 5%wt).
27746354	2	33	dep	solutions	259:267	arg1	viscosity					306:314	viscosity	306:314	viscosity	306:314	The blending solutions property, gel time, transparency and viscosity of G/CS (corn starch) and G/OCS blend solutions were compared at different ratios (10:0;9:1;8:2;7:3;6:4;5:5) and concentrations(1%wt; 3%wt; 5%wt).
27746354	2	33	dep	solutions	259:267	arg1	transparency					289:300	transparency	289:300	transparency	289:300	The blending solutions property, gel time, transparency and viscosity of G/CS (corn starch) and G/OCS blend solutions were compared at different ratios (10:0;9:1;8:2;7:3;6:4;5:5) and concentrations(1%wt; 3%wt; 5%wt).
27746354	0	34	theme	corn	52:55	arg1	starch					57:62	gelatine/oxidized corn starch	34:62	gelatine/oxidized corn starch	34:62	Preparation and characteristic of gelatine/oxidized corn starch and gelatin/corn starch blend microspheres.
27746354	5	35	theme	G/OCS	698:702	arg1	solutions					685:693	Blends solutions	678:693	Blends solutions of G/OCS	678:702	Blends solutions of G/OCS showed longer Gel time, higher transparency and lower viscosity; further displayed faster dissolution rate than that of G/CS under similar conditions.
27746354	1	36	theme	new	186:188	arg1	Combinations					108:119	Combinations	108:119	Combinations of gelatin (G) and oxidized corn starch (OCS)	108:165	Combinations of gelatin (G) and oxidized corn starch (OCS) were explored as a new microcapsule composite for single droplet spray drying.
27746354	1	36	theme	new	186:188	arg1	composite					203:211	a new microcapsule composite	184:211	a new microcapsule composite for single droplet spray drying	184:243	Combinations of gelatin (G) and oxidized corn starch (OCS) were explored as a new microcapsule composite for single droplet spray drying.
27746354	0	37	theme	gelatine/oxidized	34:50	arg1	starch					57:62	gelatine/oxidized corn starch	34:62	gelatine/oxidized corn starch	34:62	Preparation and characteristic of gelatine/oxidized corn starch and gelatin/corn starch blend microspheres.
27746354	4	38	theme	FTIR	661:664	arg1	techniques					666:675	FTIR techniques	661:675	FTIR techniques	661:675	Possible reaction mechanisms in the composite blend were elucidated by SEM and FTIR techniques.
27746354	1	39	theme	microcapsule	190:201	arg1	Combinations					108:119	Combinations	108:119	Combinations of gelatin (G) and oxidized corn starch (OCS)	108:165	Combinations of gelatin (G) and oxidized corn starch (OCS) were explored as a new microcapsule composite for single droplet spray drying.
27746354	1	39	theme	microcapsule	190:201	arg1	composite					203:211	a new microcapsule composite	184:211	a new microcapsule composite for single droplet spray drying	184:243	Combinations of gelatin (G) and oxidized corn starch (OCS) were explored as a new microcapsule composite for single droplet spray drying.
27746354	5	40	theme	higher	728:733	arg1	transparency					735:746	higher transparency	728:746	higher transparency	728:746	Blends solutions of G/OCS showed longer Gel time, higher transparency and lower viscosity; further displayed faster dissolution rate than that of G/CS under similar conditions.
27746354	2	41	theme	wt	452:453	arg1	%					457:457	1%wt; 3%wt; 5%wt	444:459	1%wt; 3%wt; 5%wt	444:459	The blending solutions property, gel time, transparency and viscosity of G/CS (corn starch) and G/OCS blend solutions were compared at different ratios (10:0;9:1;8:2;7:3;6:4;5:5) and concentrations(1%wt; 3%wt; 5%wt).
27746354	2	41	theme	wt	452:453	arg1	concentrations					429:442	concentrations	429:442	concentrations(1%wt; 3%wt; 5%wt)	429:460	The blending solutions property, gel time, transparency and viscosity of G/CS (corn starch) and G/OCS blend solutions were compared at different ratios (10:0;9:1;8:2;7:3;6:4;5:5) and concentrations(1%wt; 3%wt; 5%wt).
27746354	3	42	theme	dissolution	478:488	arg1	behaviors					490:498	The drying and dissolution behaviors	463:498	The drying and dissolution behaviors of composite droplet	463:519	The drying and dissolution behaviors of composite droplet have been studied using the single droplet drying technique.
27746354	2	43	theme	G/CS	319:322	arg1	property					269:276	property	269:276	property	269:276	The blending solutions property, gel time, transparency and viscosity of G/CS (corn starch) and G/OCS blend solutions were compared at different ratios (10:0;9:1;8:2;7:3;6:4;5:5) and concentrations(1%wt; 3%wt; 5%wt).
27746354	2	43	theme	G/CS	319:322	arg1	time					283:286	gel time	279:286	gel time	279:286	The blending solutions property, gel time, transparency and viscosity of G/CS (corn starch) and G/OCS blend solutions were compared at different ratios (10:0;9:1;8:2;7:3;6:4;5:5) and concentrations(1%wt; 3%wt; 5%wt).
27746354	2	43	theme	G/CS	319:322	arg1	viscosity					306:314	viscosity	306:314	viscosity	306:314	The blending solutions property, gel time, transparency and viscosity of G/CS (corn starch) and G/OCS blend solutions were compared at different ratios (10:0;9:1;8:2;7:3;6:4;5:5) and concentrations(1%wt; 3%wt; 5%wt).
27746354	2	43	theme	G/CS	319:322	arg1	solutions					259:267	The blending solutions	246:267	The blending solutions property, gel time, transparency and viscosity of G/CS (corn starch) and G/OCS blend solutions	246:362	The blending solutions property, gel time, transparency and viscosity of G/CS (corn starch) and G/OCS blend solutions were compared at different ratios (10:0;9:1;8:2;7:3;6:4;5:5) and concentrations(1%wt; 3%wt; 5%wt).
27746354	2	43	theme	G/CS	319:322	arg1	transparency					289:300	transparency	289:300	transparency	289:300	The blending solutions property, gel time, transparency and viscosity of G/CS (corn starch) and G/OCS blend solutions were compared at different ratios (10:0;9:1;8:2;7:3;6:4;5:5) and concentrations(1%wt; 3%wt; 5%wt).
27746354	0	44	theme	starch	57:62	arg1	characteristic					16:29	characteristic	16:29	characteristic	16:29	Preparation and characteristic of gelatine/oxidized corn starch and gelatin/corn starch blend microspheres.
27746354	0	44	theme	starch	57:62	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characteristic of gelatine/oxidized corn starch and gelatin/corn starch blend microspheres.
27746354	2	45	theme	%	451:451	arg1	%					457:457	1%wt; 3%wt; 5%wt	444:459	1%wt; 3%wt; 5%wt	444:459	The blending solutions property, gel time, transparency and viscosity of G/CS (corn starch) and G/OCS blend solutions were compared at different ratios (10:0;9:1;8:2;7:3;6:4;5:5) and concentrations(1%wt; 3%wt; 5%wt).
27746354	2	45	theme	%	451:451	arg1	concentrations					429:442	concentrations	429:442	concentrations(1%wt; 3%wt; 5%wt)	429:460	The blending solutions property, gel time, transparency and viscosity of G/CS (corn starch) and G/OCS blend solutions were compared at different ratios (10:0;9:1;8:2;7:3;6:4;5:5) and concentrations(1%wt; 3%wt; 5%wt).
27746354	7	46	theme	thermodynamic	1156:1168	arg1	incompatibility					1170:1184	thermodynamic incompatibility	1156:1184	thermodynamic incompatibility	1156:1184	All results indicated that the composites could be prepared with excellent properties by G/OCS (6:4) which would overcome some disadvantage such as thermodynamic incompatibility and phase separation by G/CS.
27746354	7	47	theme	phase	1190:1194	arg1	separation					1196:1205	phase separation	1190:1205	phase separation by G/CS	1190:1213	All results indicated that the composites could be prepared with excellent properties by G/OCS (6:4) which would overcome some disadvantage such as thermodynamic incompatibility and phase separation by G/CS.
27746354	5	48	theme	similar	835:841	arg1	conditions					843:852	similar conditions	835:852	similar conditions	835:852	Blends solutions of G/OCS showed longer Gel time, higher transparency and lower viscosity; further displayed faster dissolution rate than that of G/CS under similar conditions.
27746354	6	49	theme	G	954:954	arg1	G					954:954	G	954:954	G which improved the compatibility between G and OCS	954:1005	This was attributed to the formed Schiff base between the aldehyde group of OCS and amino group of G which improved the compatibility between G and OCS.
27746354	6	49	theme	G	954:954	arg1	OCS					931:933	OCS	931:933	OCS	931:933	This was attributed to the formed Schiff base between the aldehyde group of OCS and amino group of G which improved the compatibility between G and OCS.
27746354	6	49	theme	G	954:954	arg1	group					945:949	amino group	939:949	amino group of G which improved the compatibility between G and OCS	939:1005	This was attributed to the formed Schiff base between the aldehyde group of OCS and amino group of G which improved the compatibility between G and OCS.
27746354	6	49	theme	G	954:954	arg1	group					922:926	the aldehyde group	909:926	the aldehyde group of OCS	909:933	This was attributed to the formed Schiff base between the aldehyde group of OCS and amino group of G which improved the compatibility between G and OCS.
27746354	3	50	theme	composite	503:511	arg1	droplet					513:519	composite droplet	503:519	composite droplet	503:519	The drying and dissolution behaviors of composite droplet have been studied using the single droplet drying technique.
27746354	3	51	theme	drying	467:472	arg1	behaviors					490:498	The drying and dissolution behaviors	463:498	The drying and dissolution behaviors of composite droplet	463:519	The drying and dissolution behaviors of composite droplet have been studied using the single droplet drying technique.
27746354	6	52	theme	amino	939:943	arg1	G					954:954	G	954:954	G which improved the compatibility between G and OCS	954:1005	This was attributed to the formed Schiff base between the aldehyde group of OCS and amino group of G which improved the compatibility between G and OCS.
27746354	6	52	theme	amino	939:943	arg1	OCS					931:933	OCS	931:933	OCS	931:933	This was attributed to the formed Schiff base between the aldehyde group of OCS and amino group of G which improved the compatibility between G and OCS.
27746354	6	52	theme	amino	939:943	arg1	group					945:949	amino group	939:949	amino group of G which improved the compatibility between G and OCS	939:1005	This was attributed to the formed Schiff base between the aldehyde group of OCS and amino group of G which improved the compatibility between G and OCS.
27746354	0	53	theme	starch	81:86	arg1	microspheres					94:105	gelatin/corn starch blend microspheres	68:105	gelatin/corn starch blend microspheres	68:105	Preparation and characteristic of gelatine/oxidized corn starch and gelatin/corn starch blend microspheres.
27746354	3	54	theme	droplet	513:519	arg1	behaviors					490:498	The drying and dissolution behaviors	463:498	The drying and dissolution behaviors of composite droplet	463:519	The drying and dissolution behaviors of composite droplet have been studied using the single droplet drying technique.
27746354	1	55	theme	oxidized	140:147	arg1	OCS					162:164	OCS	162:164	OCS	162:164	Combinations of gelatin (G) and oxidized corn starch (OCS) were explored as a new microcapsule composite for single droplet spray drying.
27746354	1	55	theme	oxidized	140:147	arg1	starch					154:159	oxidized corn starch	140:159	oxidized corn starch (OCS)	140:165	Combinations of gelatin (G) and oxidized corn starch (OCS) were explored as a new microcapsule composite for single droplet spray drying.
27746354	5	56	theme	Gel	718:720	arg1	time					722:725	longer Gel time	711:725	longer Gel time	711:725	Blends solutions of G/OCS showed longer Gel time, higher transparency and lower viscosity; further displayed faster dissolution rate than that of G/CS under similar conditions.
27746354	1	57	theme	single	217:222	arg1	drying					238:243	single droplet spray drying	217:243	single droplet spray drying	217:243	Combinations of gelatin (G) and oxidized corn starch (OCS) were explored as a new microcapsule composite for single droplet spray drying.
27746354	0	58	theme	gelatin/corn	68:79	arg1	microspheres					94:105	gelatin/corn starch blend microspheres	68:105	gelatin/corn starch blend microspheres	68:105	Preparation and characteristic of gelatine/oxidized corn starch and gelatin/corn starch blend microspheres.
27746354	5	59	theme	Blends	678:683	arg1	solutions					685:693	Blends solutions	678:693	Blends solutions of G/OCS	678:702	Blends solutions of G/OCS showed longer Gel time, higher transparency and lower viscosity; further displayed faster dissolution rate than that of G/CS under similar conditions.
27746354	1	60	theme	corn	149:152	arg1	OCS					162:164	OCS	162:164	OCS	162:164	Combinations of gelatin (G) and oxidized corn starch (OCS) were explored as a new microcapsule composite for single droplet spray drying.
27746354	1	60	theme	corn	149:152	arg1	starch					154:159	oxidized corn starch	140:159	oxidized corn starch (OCS)	140:165	Combinations of gelatin (G) and oxidized corn starch (OCS) were explored as a new microcapsule composite for single droplet spray drying.
27746354	1	61	theme	droplet	224:230	arg1	drying					238:243	single droplet spray drying	217:243	single droplet spray drying	217:243	Combinations of gelatin (G) and oxidized corn starch (OCS) were explored as a new microcapsule composite for single droplet spray drying.
27987999	0	0	theme	biofriendly	75:85	arg1	hydrogel					87:94	biofriendly hydrogel	75:94	biofriendly hydrogel of nano cubic topology	75:117	Dispersion of chitosan in liquid crystalline lamellar phase: Production of biofriendly hydrogel of nano cubic topology.
27987999	6	1	dep	acquired	841:848	arg1	primitive					850:858	primitive	850:858	primitive	850:858	However a higher 0.6wt% evolved this phase to a newly acquired primitive Im3m topology.
27987999	2	2	dep	constant	307:314	arg1	delimited					350:358	delimited	350:358	is delimited by incorporation of polymer molecules into the structure	347:415	The ratio of lipid/water was constant and swelling of lipids bilayer is delimited by incorporation of polymer molecules into the structure.
27987999	1	3	theme	organization	264:275	arg1	composites					232:241	composites	232:241	composites of defined molecular organization	232:275	Bicontinuous cubic phases were produced with introduction of chitosan in phospholipid/water hydrogel, providing composites of defined molecular organization.
27987999	1	3	theme	organization	264:275	arg1	organization					264:275	defined molecular organization	246:275	defined molecular organization	246:275	Bicontinuous cubic phases were produced with introduction of chitosan in phospholipid/water hydrogel, providing composites of defined molecular organization.
27987999	5	4	theme	0.4wt	723:727	arg1	chitosan					730:737	0.4wt% chitosan	723:737	0.4wt% chitosan	723:737	An increment to 0.4wt% chitosan caused large increase in Pn3m lattice distance.
27987999	4	5	theme	crystalline	544:554	arg1	phase					568:572	The expected liquid crystalline Lα lamellar phase	524:572	The expected liquid crystalline Lα lamellar phase	524:572	The expected liquid crystalline Lα lamellar phase was suppressed by 0.2wt% chitosan leading to formation of diamond Pn3m and gyroid Ia3d cubic topology, with close lattice distances.
27987999	8	6	theme	composite	1057:1065	arg1	hydrogel					1067:1074	The composite hydrogel	1053:1074	The composite hydrogel of specific topologies	1053:1097	The composite hydrogel of specific topologies presents reduced time release of gallic acid and may find application as new material for time-sustained delivery of bioactive compounds.
27987999	7	7	theme	chitosan	978:985	arg1	requirements					996:1007	chitosan physical requirements	978:1007	chitosan physical requirements	978:1007	The structuring process of the three-dimensional complex network is principally governed by demands of chitosan physical requirements over lipids bilayers interfacial curvature.
27987999	8	8	theme	new	1172:1174	arg1	material					1176:1183	new material	1172:1183	new material for time-sustained delivery of bioactive compounds	1172:1234	The composite hydrogel of specific topologies presents reduced time release of gallic acid and may find application as new material for time-sustained delivery of bioactive compounds.
27987999	0	9	from	Dispersion	0:9	arg1	phase					54:58	liquid crystalline lamellar phase	26:58	liquid crystalline lamellar phase	26:58	Dispersion of chitosan in liquid crystalline lamellar phase: Production of biofriendly hydrogel of nano cubic topology.
27987999	8	10	theme	specific	1079:1086	arg1	topologies					1088:1097	specific topologies	1079:1097	specific topologies	1079:1097	The composite hydrogel of specific topologies presents reduced time release of gallic acid and may find application as new material for time-sustained delivery of bioactive compounds.
27987999	7	11	theme	physical	987:994	arg1	requirements					996:1007	chitosan physical requirements	978:1007	chitosan physical requirements	978:1007	The structuring process of the three-dimensional complex network is principally governed by demands of chitosan physical requirements over lipids bilayers interfacial curvature.
27987999	7	12	theme	structuring	879:889	arg1	process					891:897	The structuring process	875:897	The structuring process of the three-dimensional complex network	875:938	The structuring process of the three-dimensional complex network is principally governed by demands of chitosan physical requirements over lipids bilayers interfacial curvature.
27987999	0	13	theme	hydrogel	87:94	arg1	Production					61:70	Production	61:70	Dispersion of chitosan in liquid crystalline lamellar phase: Production of biofriendly hydrogel of nano cubic topology.	0:118	Dispersion of chitosan in liquid crystalline lamellar phase: Production of biofriendly hydrogel of nano cubic topology.
27987999	3	14	theme	cubic	510:514	arg1	phases					516:521	coexisting cubic phases	499:521	coexisting cubic phases	499:521	By means of synchrotron small angle X-ray scattering we identified topologies of coexisting cubic phases.
27987999	0	15	theme	cubic	104:108	arg1	topology					110:117	nano cubic topology	99:117	nano cubic topology	99:117	Dispersion of chitosan in liquid crystalline lamellar phase: Production of biofriendly hydrogel of nano cubic topology.
27987999	8	16	theme	time-sustained	1189:1202	arg1	delivery					1204:1211	time-sustained delivery	1189:1211	time-sustained delivery of bioactive compounds	1189:1234	The composite hydrogel of specific topologies presents reduced time release of gallic acid and may find application as new material for time-sustained delivery of bioactive compounds.
27987999	2	17	theme	lipid/water	291:301	arg1	constant					307:314	constant	307:314	constant	307:314	The ratio of lipid/water was constant and swelling of lipids bilayer is delimited by incorporation of polymer molecules into the structure.
27987999	2	17	theme	lipid/water	291:301	arg1	ratio					282:286	The ratio	278:286	The ratio of lipid/water	278:301	The ratio of lipid/water was constant and swelling of lipids bilayer is delimited by incorporation of polymer molecules into the structure.
27987999	3	18	theme	small	442:446	arg1	scattering					460:469	synchrotron small angle X-ray scattering	430:469	synchrotron small angle X-ray scattering	430:469	By means of synchrotron small angle X-ray scattering we identified topologies of coexisting cubic phases.
27987999	3	19	theme	X-ray	454:458	arg1	scattering					460:469	synchrotron small angle X-ray scattering	430:469	synchrotron small angle X-ray scattering	430:469	By means of synchrotron small angle X-ray scattering we identified topologies of coexisting cubic phases.
27987999	0	20	theme	nano	99:102	arg1	topology					110:117	nano cubic topology	99:117	nano cubic topology	99:117	Dispersion of chitosan in liquid crystalline lamellar phase: Production of biofriendly hydrogel of nano cubic topology.
27987999	8	21	theme	topologies	1088:1097	arg1	hydrogel					1067:1074	The composite hydrogel	1053:1074	The composite hydrogel of specific topologies	1053:1097	The composite hydrogel of specific topologies presents reduced time release of gallic acid and may find application as new material for time-sustained delivery of bioactive compounds.
27987999	6	22	theme	0.6wt	804:808	arg1	%					809:809	a higher 0.6wt%	795:809	a higher 0.6wt%	795:809	However a higher 0.6wt% evolved this phase to a newly acquired primitive Im3m topology.
27987999	8	23	theme	compounds	1226:1234	arg1	delivery					1204:1211	time-sustained delivery	1189:1211	time-sustained delivery of bioactive compounds	1189:1234	The composite hydrogel of specific topologies presents reduced time release of gallic acid and may find application as new material for time-sustained delivery of bioactive compounds.
27987999	8	24	theme	bioactive	1216:1224	arg1	compounds					1226:1234	bioactive compounds	1216:1234	bioactive compounds	1216:1234	The composite hydrogel of specific topologies presents reduced time release of gallic acid and may find application as new material for time-sustained delivery of bioactive compounds.
27987999	4	25	theme	0.2wt	592:596	arg1	chitosan					599:606	0.2wt% chitosan	592:606	0.2wt% chitosan leading to formation of diamond Pn3m and gyroid Ia3d cubic topology	592:674	The expected liquid crystalline Lα lamellar phase was suppressed by 0.2wt% chitosan leading to formation of diamond Pn3m and gyroid Ia3d cubic topology, with close lattice distances.
27987999	8	26	theme	time	1116:1119	arg1	release					1121:1127	reduced time release	1108:1127	reduced time release of gallic acid	1108:1142	The composite hydrogel of specific topologies presents reduced time release of gallic acid and may find application as new material for time-sustained delivery of bioactive compounds.
27987999	6	27	theme	higher	797:802	arg1	%					809:809	a higher 0.6wt%	795:809	a higher 0.6wt%	795:809	However a higher 0.6wt% evolved this phase to a newly acquired primitive Im3m topology.
27987999	2	28	theme	lipids	332:337	arg1	swelling					320:327	swelling	320:327	swelling	320:327	The ratio of lipid/water was constant and swelling of lipids bilayer is delimited by incorporation of polymer molecules into the structure.
27987999	2	28	theme	lipids	332:337	arg1	constant					307:314	constant	307:314	constant	307:314	The ratio of lipid/water was constant and swelling of lipids bilayer is delimited by incorporation of polymer molecules into the structure.
27987999	2	28	theme	lipids	332:337	arg1	ratio					282:286	The ratio	278:286	The ratio of lipid/water	278:301	The ratio of lipid/water was constant and swelling of lipids bilayer is delimited by incorporation of polymer molecules into the structure.
27987999	1	29	theme	chitosan	181:188	arg1	introduction					165:176	introduction	165:176	introduction of chitosan in phospholipid/water hydrogel	165:219	Bicontinuous cubic phases were produced with introduction of chitosan in phospholipid/water hydrogel, providing composites of defined molecular organization.
27987999	8	30	theme	acid	1139:1142	arg1	release					1121:1127	reduced time release	1108:1127	reduced time release of gallic acid	1108:1142	The composite hydrogel of specific topologies presents reduced time release of gallic acid and may find application as new material for time-sustained delivery of bioactive compounds.
27987999	8	31	theme	reduced	1108:1114	arg1	release					1121:1127	reduced time release	1108:1127	reduced time release of gallic acid	1108:1142	The composite hydrogel of specific topologies presents reduced time release of gallic acid and may find application as new material for time-sustained delivery of bioactive compounds.
27987999	7	32	dep	requirements	996:1007	arg1	demands					967:973	demands	967:973	demands	967:973	The structuring process of the three-dimensional complex network is principally governed by demands of chitosan physical requirements over lipids bilayers interfacial curvature.
27987999	3	33	theme	angle	448:452	arg1	scattering					460:469	synchrotron small angle X-ray scattering	430:469	synchrotron small angle X-ray scattering	430:469	By means of synchrotron small angle X-ray scattering we identified topologies of coexisting cubic phases.
27987999	1	34	theme	phospholipid/water	193:210	arg1	hydrogel					212:219	phospholipid/water hydrogel	193:219	phospholipid/water hydrogel	193:219	Bicontinuous cubic phases were produced with introduction of chitosan in phospholipid/water hydrogel, providing composites of defined molecular organization.
27987999	0	35	theme	chitosan	14:21	arg1	Dispersion					0:9	Dispersion	0:9	Dispersion of chitosan in liquid crystalline lamellar phase: Production of biofriendly hydrogel of nano cubic topology.	0:118	Dispersion of chitosan in liquid crystalline lamellar phase: Production of biofriendly hydrogel of nano cubic topology.
27987999	4	36	theme	%	597:597	arg1	chitosan					599:606	0.2wt% chitosan	592:606	0.2wt% chitosan leading to formation of diamond Pn3m and gyroid Ia3d cubic topology	592:674	The expected liquid crystalline Lα lamellar phase was suppressed by 0.2wt% chitosan leading to formation of diamond Pn3m and gyroid Ia3d cubic topology, with close lattice distances.
27987999	0	37	theme	topology	110:117	arg1	hydrogel					87:94	biofriendly hydrogel	75:94	biofriendly hydrogel of nano cubic topology	75:117	Dispersion of chitosan in liquid crystalline lamellar phase: Production of biofriendly hydrogel of nano cubic topology.
27987999	5	38	from	increase	752:759	arg1	distance					777:784	Pn3m lattice distance	764:784	Pn3m lattice distance	764:784	An increment to 0.4wt% chitosan caused large increase in Pn3m lattice distance.
27987999	0	39	theme	crystalline	33:43	arg1	phase					54:58	liquid crystalline lamellar phase	26:58	liquid crystalline lamellar phase	26:58	Dispersion of chitosan in liquid crystalline lamellar phase: Production of biofriendly hydrogel of nano cubic topology.
27987999	4	40	theme	lattice	688:694	arg1	distances					696:704	close lattice distances	682:704	close lattice distances	682:704	The expected liquid crystalline Lα lamellar phase was suppressed by 0.2wt% chitosan leading to formation of diamond Pn3m and gyroid Ia3d cubic topology, with close lattice distances.
27987999	5	41	theme	%	728:728	arg1	chitosan					730:737	0.4wt% chitosan	723:737	0.4wt% chitosan	723:737	An increment to 0.4wt% chitosan caused large increase in Pn3m lattice distance.
27987999	4	42	theme	close	682:686	arg1	distances					696:704	close lattice distances	682:704	close lattice distances	682:704	The expected liquid crystalline Lα lamellar phase was suppressed by 0.2wt% chitosan leading to formation of diamond Pn3m and gyroid Ia3d cubic topology, with close lattice distances.
27987999	0	43	theme	liquid	26:31	arg1	phase					54:58	liquid crystalline lamellar phase	26:58	liquid crystalline lamellar phase	26:58	Dispersion of chitosan in liquid crystalline lamellar phase: Production of biofriendly hydrogel of nano cubic topology.
27987999	4	44	theme	lamellar	559:566	arg1	phase					568:572	The expected liquid crystalline Lα lamellar phase	524:572	The expected liquid crystalline Lα lamellar phase	524:572	The expected liquid crystalline Lα lamellar phase was suppressed by 0.2wt% chitosan leading to formation of diamond Pn3m and gyroid Ia3d cubic topology, with close lattice distances.
27987999	3	45	theme	synchrotron	430:440	arg1	scattering					460:469	synchrotron small angle X-ray scattering	430:469	synchrotron small angle X-ray scattering	430:469	By means of synchrotron small angle X-ray scattering we identified topologies of coexisting cubic phases.
27987999	5	46	theme	large	746:750	arg1	increase					752:759	large increase	746:759	large increase in Pn3m lattice distance	746:784	An increment to 0.4wt% chitosan caused large increase in Pn3m lattice distance.
27987999	4	47	theme	cubic	661:665	arg1	topology					667:674	cubic topology	661:674	cubic topology	661:674	The expected liquid crystalline Lα lamellar phase was suppressed by 0.2wt% chitosan leading to formation of diamond Pn3m and gyroid Ia3d cubic topology, with close lattice distances.
27987999	4	48	theme	Lα	556:557	arg1	phase					568:572	The expected liquid crystalline Lα lamellar phase	524:572	The expected liquid crystalline Lα lamellar phase	524:572	The expected liquid crystalline Lα lamellar phase was suppressed by 0.2wt% chitosan leading to formation of diamond Pn3m and gyroid Ia3d cubic topology, with close lattice distances.
27987999	4	49	theme	Pn3m	640:643	arg1	formation					619:627	formation	619:627	formation of diamond Pn3m and gyroid Ia3d cubic topology	619:674	The expected liquid crystalline Lα lamellar phase was suppressed by 0.2wt% chitosan leading to formation of diamond Pn3m and gyroid Ia3d cubic topology, with close lattice distances.
27987999	3	50	theme	phases	516:521	arg1	topologies					485:494	topologies	485:494	topologies of coexisting cubic phases	485:521	By means of synchrotron small angle X-ray scattering we identified topologies of coexisting cubic phases.
27987999	0	51	theme	lamellar	45:52	arg1	phase					54:58	liquid crystalline lamellar phase	26:58	liquid crystalline lamellar phase	26:58	Dispersion of chitosan in liquid crystalline lamellar phase: Production of biofriendly hydrogel of nano cubic topology.
27987999	6	52	theme	acquired	841:848	arg1	topology					865:872	a newly acquired primitive Im3m topology	833:872	a newly acquired primitive Im3m topology	833:872	However a higher 0.6wt% evolved this phase to a newly acquired primitive Im3m topology.
27987999	7	53	theme	interfacial	1030:1040	arg1	curvature					1042:1050	interfacial curvature	1030:1050	interfacial curvature	1030:1050	The structuring process of the three-dimensional complex network is principally governed by demands of chitosan physical requirements over lipids bilayers interfacial curvature.
27987999	6	54	theme	Im3m	860:863	arg1	topology					865:872	a newly acquired primitive Im3m topology	833:872	a newly acquired primitive Im3m topology	833:872	However a higher 0.6wt% evolved this phase to a newly acquired primitive Im3m topology.
27987999	7	55	theme	complex	924:930	arg1	network					932:938	the three-dimensional complex network	902:938	the three-dimensional complex network	902:938	The structuring process of the three-dimensional complex network is principally governed by demands of chitosan physical requirements over lipids bilayers interfacial curvature.
27987999	4	56	dep	Pn3m	640:643	arg1	topology					667:674	cubic topology	661:674	cubic topology	661:674	The expected liquid crystalline Lα lamellar phase was suppressed by 0.2wt% chitosan leading to formation of diamond Pn3m and gyroid Ia3d cubic topology, with close lattice distances.
27987999	1	57	theme	Bicontinuous	120:131	arg1	phases					139:144	Bicontinuous cubic phases	120:144	Bicontinuous cubic phases	120:144	Bicontinuous cubic phases were produced with introduction of chitosan in phospholipid/water hydrogel, providing composites of defined molecular organization.
27987999	7	58	theme	network	932:938	arg1	process					891:897	The structuring process	875:897	The structuring process of the three-dimensional complex network	875:938	The structuring process of the three-dimensional complex network is principally governed by demands of chitosan physical requirements over lipids bilayers interfacial curvature.
27987999	4	59	theme	liquid	537:542	arg1	phase					568:572	The expected liquid crystalline Lα lamellar phase	524:572	The expected liquid crystalline Lα lamellar phase	524:572	The expected liquid crystalline Lα lamellar phase was suppressed by 0.2wt% chitosan leading to formation of diamond Pn3m and gyroid Ia3d cubic topology, with close lattice distances.
27987999	7	60	theme	lipids	1014:1019	arg1	bilayers					1021:1028	lipids bilayers	1014:1028	lipids bilayers	1014:1028	The structuring process of the three-dimensional complex network is principally governed by demands of chitosan physical requirements over lipids bilayers interfacial curvature.
27987999	1	61	theme	cubic	133:137	arg1	phases					139:144	Bicontinuous cubic phases	120:144	Bicontinuous cubic phases	120:144	Bicontinuous cubic phases were produced with introduction of chitosan in phospholipid/water hydrogel, providing composites of defined molecular organization.
27987999	3	62	theme	coexisting	499:508	arg1	phases					516:521	coexisting cubic phases	499:521	coexisting cubic phases	499:521	By means of synchrotron small angle X-ray scattering we identified topologies of coexisting cubic phases.
27987999	5	63	theme	Pn3m	764:767	arg1	distance					777:784	Pn3m lattice distance	764:784	Pn3m lattice distance	764:784	An increment to 0.4wt% chitosan caused large increase in Pn3m lattice distance.
27987999	4	64	theme	Ia3d	656:659	arg1	formation					619:627	formation	619:627	formation of diamond Pn3m and gyroid Ia3d cubic topology	619:674	The expected liquid crystalline Lα lamellar phase was suppressed by 0.2wt% chitosan leading to formation of diamond Pn3m and gyroid Ia3d cubic topology, with close lattice distances.
27987999	2	65	theme	molecules	388:396	arg1	incorporation					363:375	incorporation	363:375	incorporation of polymer molecules into the structure	363:415	The ratio of lipid/water was constant and swelling of lipids bilayer is delimited by incorporation of polymer molecules into the structure.
27987999	7	66	theme	three-dimensional	906:922	arg1	network					932:938	the three-dimensional complex network	902:938	the three-dimensional complex network	902:938	The structuring process of the three-dimensional complex network is principally governed by demands of chitosan physical requirements over lipids bilayers interfacial curvature.
27987999	1	67	theme	defined	246:252	arg1	organization					264:275	defined molecular organization	246:275	defined molecular organization	246:275	Bicontinuous cubic phases were produced with introduction of chitosan in phospholipid/water hydrogel, providing composites of defined molecular organization.
27987999	5	68	theme	lattice	769:775	arg1	distance					777:784	Pn3m lattice distance	764:784	Pn3m lattice distance	764:784	An increment to 0.4wt% chitosan caused large increase in Pn3m lattice distance.
27987999	4	69	theme	expected	528:535	arg1	phase					568:572	The expected liquid crystalline Lα lamellar phase	524:572	The expected liquid crystalline Lα lamellar phase	524:572	The expected liquid crystalline Lα lamellar phase was suppressed by 0.2wt% chitosan leading to formation of diamond Pn3m and gyroid Ia3d cubic topology, with close lattice distances.
27987999	0	70	dep	Dispersion	0:9	arg1	Production					61:70	Production	61:70	Dispersion of chitosan in liquid crystalline lamellar phase: Production of biofriendly hydrogel of nano cubic topology.	0:118	Dispersion of chitosan in liquid crystalline lamellar phase: Production of biofriendly hydrogel of nano cubic topology.
27987999	1	71	from	introduction	165:176	arg1	hydrogel					212:219	phospholipid/water hydrogel	193:219	phospholipid/water hydrogel	193:219	Bicontinuous cubic phases were produced with introduction of chitosan in phospholipid/water hydrogel, providing composites of defined molecular organization.
27987999	2	72	theme	polymer	380:386	arg1	molecules					388:396	polymer molecules	380:396	polymer molecules	380:396	The ratio of lipid/water was constant and swelling of lipids bilayer is delimited by incorporation of polymer molecules into the structure.
27987999	8	73	theme	gallic	1132:1137	arg1	acid					1139:1142	gallic acid	1132:1142	gallic acid	1132:1142	The composite hydrogel of specific topologies presents reduced time release of gallic acid and may find application as new material for time-sustained delivery of bioactive compounds.
27987999	1	74	theme	molecular	254:262	arg1	organization					264:275	defined molecular organization	246:275	defined molecular organization	246:275	Bicontinuous cubic phases were produced with introduction of chitosan in phospholipid/water hydrogel, providing composites of defined molecular organization.
29239606	3	0	theme	decreased	1045:1053	arg1	content					1063:1069	a decreased β-sheet content	1043:1069	a decreased β-sheet content	1043:1069	The thermodynamics and structural data demonstrated that the midpoint temperature (Tm) and unfolding enthalpy (ΔH) of β-glucosidase was shifted to high values (76.6 °C, 126.3 J/g) in the presence of MEL-A, and the secondary structure changes of β-glucosidase, including the increased α-helix, β-turn, or random coil contents, and a decreased β-sheet content were caused by MEL-A at a CMC concentration.
29239606	4	1	theme	weak	1224:1227	arg1	interactions					1241:1252	weak hydrophobic interactions	1224:1252	weak hydrophobic interactions happened between the amino acid residues of β-glucosidase and the fatty acid residues of MEL-A	1224:1347	The further ITC and docking simulations suggested the bindings of MEL-A toward β-glucosidase were driven by weak hydrophobic interactions happened between the amino acid residues of β-glucosidase and the fatty acid residues of MEL-A, in addition to hydrogen bonds between amino acids and hydroxyl in glycosyl residues of this biosurfactant.
29239606	2	2	theme	decreased	673:681	arg1	Vmax					683:686	Vmax	683:686	Vmax	683:686	The enzyme inhibition kinetics data showed that MEL-A at a low concentration (< critical micelle concentration (CMC), 20.0 ± 5.0 μM) enhanced β-glucosidase activity, whereas it inhibited the enzyme activity at higher concentrations more than 20.0 μM, followed by a decreased Vmax and Km of β-glucosidase.
29239606	3	3	theme	β-glucosidase	831:843	arg1	enthalpy					814:821	unfolding enthalpy	804:821	unfolding enthalpy (ΔH) of β-glucosidase	804:843	The thermodynamics and structural data demonstrated that the midpoint temperature (Tm) and unfolding enthalpy (ΔH) of β-glucosidase was shifted to high values (76.6 °C, 126.3 J/g) in the presence of MEL-A, and the secondary structure changes of β-glucosidase, including the increased α-helix, β-turn, or random coil contents, and a decreased β-sheet content were caused by MEL-A at a CMC concentration.
29239606	3	3	theme	β-glucosidase	831:843	arg1	Tm					796:797	Tm	796:797	Tm	796:797	The thermodynamics and structural data demonstrated that the midpoint temperature (Tm) and unfolding enthalpy (ΔH) of β-glucosidase was shifted to high values (76.6 °C, 126.3 J/g) in the presence of MEL-A, and the secondary structure changes of β-glucosidase, including the increased α-helix, β-turn, or random coil contents, and a decreased β-sheet content were caused by MEL-A at a CMC concentration.
29239606	3	3	theme	β-glucosidase	831:843	arg1	ΔH					824:825	ΔH	824:825	ΔH	824:825	The thermodynamics and structural data demonstrated that the midpoint temperature (Tm) and unfolding enthalpy (ΔH) of β-glucosidase was shifted to high values (76.6 °C, 126.3 J/g) in the presence of MEL-A, and the secondary structure changes of β-glucosidase, including the increased α-helix, β-turn, or random coil contents, and a decreased β-sheet content were caused by MEL-A at a CMC concentration.
29239606	3	3	theme	β-glucosidase	831:843	arg1	temperature					783:793	the midpoint temperature	770:793	the midpoint temperature (Tm)	770:798	The thermodynamics and structural data demonstrated that the midpoint temperature (Tm) and unfolding enthalpy (ΔH) of β-glucosidase was shifted to high values (76.6 °C, 126.3 J/g) in the presence of MEL-A, and the secondary structure changes of β-glucosidase, including the increased α-helix, β-turn, or random coil contents, and a decreased β-sheet content were caused by MEL-A at a CMC concentration.
29239606	1	4	theme	differential	265:276	arg1	DSC					300:302	DSC	300:302	DSC	300:302	In this work, the influences of a biosurfactant, mannosylerythritol lipids-A (MEL-A) toward β-glucosidase activity and their molecular interactions were studied by using differential scanning calorimetry (DSC), circular dichroism spectroscopy (CD), isothermal titration calorimetry (ITC), and docking simulation.
29239606	1	4	theme	differential	265:276	arg1	calorimetry					287:297	differential scanning calorimetry	265:297	differential scanning calorimetry (DSC)	265:303	In this work, the influences of a biosurfactant, mannosylerythritol lipids-A (MEL-A) toward β-glucosidase activity and their molecular interactions were studied by using differential scanning calorimetry (DSC), circular dichroism spectroscopy (CD), isothermal titration calorimetry (ITC), and docking simulation.
29239606	3	5	theme	β-sheet	1055:1061	arg1	content					1063:1069	a decreased β-sheet content	1043:1069	a decreased β-sheet content	1043:1069	The thermodynamics and structural data demonstrated that the midpoint temperature (Tm) and unfolding enthalpy (ΔH) of β-glucosidase was shifted to high values (76.6 °C, 126.3 J/g) in the presence of MEL-A, and the secondary structure changes of β-glucosidase, including the increased α-helix, β-turn, or random coil contents, and a decreased β-sheet content were caused by MEL-A at a CMC concentration.
29239606	1	6	theme	isothermal	344:353	arg1	ITC					378:380	ITC	378:380	ITC	378:380	In this work, the influences of a biosurfactant, mannosylerythritol lipids-A (MEL-A) toward β-glucosidase activity and their molecular interactions were studied by using differential scanning calorimetry (DSC), circular dichroism spectroscopy (CD), isothermal titration calorimetry (ITC), and docking simulation.
29239606	1	6	theme	isothermal	344:353	arg1	calorimetry					365:375	isothermal titration calorimetry	344:375	isothermal titration calorimetry (ITC)	344:381	In this work, the influences of a biosurfactant, mannosylerythritol lipids-A (MEL-A) toward β-glucosidase activity and their molecular interactions were studied by using differential scanning calorimetry (DSC), circular dichroism spectroscopy (CD), isothermal titration calorimetry (ITC), and docking simulation.
29239606	4	7	theme	further	1120:1126	arg1	ITC					1128:1130	The further ITC and docking simulations	1116:1154	ITC	1128:1130	The further ITC and docking simulations suggested the bindings of MEL-A toward β-glucosidase were driven by weak hydrophobic interactions happened between the amino acid residues of β-glucosidase and the fatty acid residues of MEL-A, in addition to hydrogen bonds between amino acids and hydroxyl in glycosyl residues of this biosurfactant.
29239606	4	8	theme	glycosyl	1416:1423	arg1	residues					1425:1432	glycosyl residues	1416:1432	glycosyl residues of this biosurfactant	1416:1454	The further ITC and docking simulations suggested the bindings of MEL-A toward β-glucosidase were driven by weak hydrophobic interactions happened between the amino acid residues of β-glucosidase and the fatty acid residues of MEL-A, in addition to hydrogen bonds between amino acids and hydroxyl in glycosyl residues of this biosurfactant.
29239606	4	8	theme	glycosyl	1416:1423	arg1	biosurfactant					1442:1454	this biosurfactant	1437:1454	this biosurfactant	1437:1454	The further ITC and docking simulations suggested the bindings of MEL-A toward β-glucosidase were driven by weak hydrophobic interactions happened between the amino acid residues of β-glucosidase and the fatty acid residues of MEL-A, in addition to hydrogen bonds between amino acids and hydroxyl in glycosyl residues of this biosurfactant.
29239606	2	9	dep	concentrations	625:638	arg1	μM					655:656	μM	655:656	μM	655:656	The enzyme inhibition kinetics data showed that MEL-A at a low concentration (< critical micelle concentration (CMC), 20.0 ± 5.0 μM) enhanced β-glucosidase activity, whereas it inhibited the enzyme activity at higher concentrations more than 20.0 μM, followed by a decreased Vmax and Km of β-glucosidase.
29239606	1	10	theme	scanning	278:285	arg1	DSC					300:302	DSC	300:302	DSC	300:302	In this work, the influences of a biosurfactant, mannosylerythritol lipids-A (MEL-A) toward β-glucosidase activity and their molecular interactions were studied by using differential scanning calorimetry (DSC), circular dichroism spectroscopy (CD), isothermal titration calorimetry (ITC), and docking simulation.
29239606	1	10	theme	scanning	278:285	arg1	calorimetry					287:297	differential scanning calorimetry	265:297	differential scanning calorimetry (DSC)	265:303	In this work, the influences of a biosurfactant, mannosylerythritol lipids-A (MEL-A) toward β-glucosidase activity and their molecular interactions were studied by using differential scanning calorimetry (DSC), circular dichroism spectroscopy (CD), isothermal titration calorimetry (ITC), and docking simulation.
29239606	4	11	theme	fatty	1320:1324	arg1	MEL-A					1343:1347	MEL-A	1343:1347	MEL-A	1343:1347	The further ITC and docking simulations suggested the bindings of MEL-A toward β-glucosidase were driven by weak hydrophobic interactions happened between the amino acid residues of β-glucosidase and the fatty acid residues of MEL-A, in addition to hydrogen bonds between amino acids and hydroxyl in glycosyl residues of this biosurfactant.
29239606	4	11	theme	fatty	1320:1324	arg1	residues					1331:1338	the fatty acid residues	1316:1338	the fatty acid residues of MEL-A	1316:1347	The further ITC and docking simulations suggested the bindings of MEL-A toward β-glucosidase were driven by weak hydrophobic interactions happened between the amino acid residues of β-glucosidase and the fatty acid residues of MEL-A, in addition to hydrogen bonds between amino acids and hydroxyl in glycosyl residues of this biosurfactant.
29239606	4	11	theme	fatty	1320:1324	arg1	β-glucosidase					1298:1310	β-glucosidase	1298:1310	β-glucosidase	1298:1310	The further ITC and docking simulations suggested the bindings of MEL-A toward β-glucosidase were driven by weak hydrophobic interactions happened between the amino acid residues of β-glucosidase and the fatty acid residues of MEL-A, in addition to hydrogen bonds between amino acids and hydroxyl in glycosyl residues of this biosurfactant.
29239606	1	12	theme	titration	355:363	arg1	ITC					378:380	ITC	378:380	ITC	378:380	In this work, the influences of a biosurfactant, mannosylerythritol lipids-A (MEL-A) toward β-glucosidase activity and their molecular interactions were studied by using differential scanning calorimetry (DSC), circular dichroism spectroscopy (CD), isothermal titration calorimetry (ITC), and docking simulation.
29239606	1	12	theme	titration	355:363	arg1	calorimetry					365:375	isothermal titration calorimetry	344:375	isothermal titration calorimetry (ITC)	344:381	In this work, the influences of a biosurfactant, mannosylerythritol lipids-A (MEL-A) toward β-glucosidase activity and their molecular interactions were studied by using differential scanning calorimetry (DSC), circular dichroism spectroscopy (CD), isothermal titration calorimetry (ITC), and docking simulation.
29239606	3	13	theme	β-glucosidase	958:970	arg1	content					1063:1069	a decreased β-sheet content	1043:1069	a decreased β-sheet content	1043:1069	The thermodynamics and structural data demonstrated that the midpoint temperature (Tm) and unfolding enthalpy (ΔH) of β-glucosidase was shifted to high values (76.6 °C, 126.3 J/g) in the presence of MEL-A, and the secondary structure changes of β-glucosidase, including the increased α-helix, β-turn, or random coil contents, and a decreased β-sheet content were caused by MEL-A at a CMC concentration.
29239606	3	13	theme	β-glucosidase	958:970	arg1	α-helix					997:1003	the increased α-helix	983:1003	the increased α-helix	983:1003	The thermodynamics and structural data demonstrated that the midpoint temperature (Tm) and unfolding enthalpy (ΔH) of β-glucosidase was shifted to high values (76.6 °C, 126.3 J/g) in the presence of MEL-A, and the secondary structure changes of β-glucosidase, including the increased α-helix, β-turn, or random coil contents, and a decreased β-sheet content were caused by MEL-A at a CMC concentration.
29239606	3	13	theme	β-glucosidase	958:970	arg1	changes					947:953	the secondary structure changes	923:953	the secondary structure changes	923:953	The thermodynamics and structural data demonstrated that the midpoint temperature (Tm) and unfolding enthalpy (ΔH) of β-glucosidase was shifted to high values (76.6 °C, 126.3 J/g) in the presence of MEL-A, and the secondary structure changes of β-glucosidase, including the increased α-helix, β-turn, or random coil contents, and a decreased β-sheet content were caused by MEL-A at a CMC concentration.
29239606	3	13	theme	β-glucosidase	958:970	arg1	contents					1029:1036	random coil contents	1017:1036	random coil contents	1017:1036	The thermodynamics and structural data demonstrated that the midpoint temperature (Tm) and unfolding enthalpy (ΔH) of β-glucosidase was shifted to high values (76.6 °C, 126.3 J/g) in the presence of MEL-A, and the secondary structure changes of β-glucosidase, including the increased α-helix, β-turn, or random coil contents, and a decreased β-sheet content were caused by MEL-A at a CMC concentration.
29239606	3	13	theme	β-glucosidase	958:970	arg1	β-turn					1006:1011	β-turn	1006:1011	β-turn	1006:1011	The thermodynamics and structural data demonstrated that the midpoint temperature (Tm) and unfolding enthalpy (ΔH) of β-glucosidase was shifted to high values (76.6 °C, 126.3 J/g) in the presence of MEL-A, and the secondary structure changes of β-glucosidase, including the increased α-helix, β-turn, or random coil contents, and a decreased β-sheet content were caused by MEL-A at a CMC concentration.
29239606	2	14	theme	higher	618:623	arg1	concentrations					625:638	higher concentrations	618:638	higher concentrations more than 20.0 μM	618:656	The enzyme inhibition kinetics data showed that MEL-A at a low concentration (< critical micelle concentration (CMC), 20.0 ± 5.0 μM) enhanced β-glucosidase activity, whereas it inhibited the enzyme activity at higher concentrations more than 20.0 μM, followed by a decreased Vmax and Km of β-glucosidase.
29239606	3	15	dep	°C	878:879	arg1	J/g					888:890	126.3 J/g	882:890	126.3 J/g	882:890	The thermodynamics and structural data demonstrated that the midpoint temperature (Tm) and unfolding enthalpy (ΔH) of β-glucosidase was shifted to high values (76.6 °C, 126.3 J/g) in the presence of MEL-A, and the secondary structure changes of β-glucosidase, including the increased α-helix, β-turn, or random coil contents, and a decreased β-sheet content were caused by MEL-A at a CMC concentration.
29239606	2	16	theme	β-glucosidase	550:562	arg1	activity					564:571	β-glucosidase activity	550:571	β-glucosidase activity	550:571	The enzyme inhibition kinetics data showed that MEL-A at a low concentration (< critical micelle concentration (CMC), 20.0 ± 5.0 μM) enhanced β-glucosidase activity, whereas it inhibited the enzyme activity at higher concentrations more than 20.0 μM, followed by a decreased Vmax and Km of β-glucosidase.
29239606	3	17	theme	MEL-A	912:916	arg1	presence					900:907	the presence	896:907	the presence of MEL-A	896:916	The thermodynamics and structural data demonstrated that the midpoint temperature (Tm) and unfolding enthalpy (ΔH) of β-glucosidase was shifted to high values (76.6 °C, 126.3 J/g) in the presence of MEL-A, and the secondary structure changes of β-glucosidase, including the increased α-helix, β-turn, or random coil contents, and a decreased β-sheet content were caused by MEL-A at a CMC concentration.
29239606	4	18	theme	acid	1326:1329	arg1	MEL-A					1343:1347	MEL-A	1343:1347	MEL-A	1343:1347	The further ITC and docking simulations suggested the bindings of MEL-A toward β-glucosidase were driven by weak hydrophobic interactions happened between the amino acid residues of β-glucosidase and the fatty acid residues of MEL-A, in addition to hydrogen bonds between amino acids and hydroxyl in glycosyl residues of this biosurfactant.
29239606	4	18	theme	acid	1326:1329	arg1	residues					1331:1338	the fatty acid residues	1316:1338	the fatty acid residues of MEL-A	1316:1347	The further ITC and docking simulations suggested the bindings of MEL-A toward β-glucosidase were driven by weak hydrophobic interactions happened between the amino acid residues of β-glucosidase and the fatty acid residues of MEL-A, in addition to hydrogen bonds between amino acids and hydroxyl in glycosyl residues of this biosurfactant.
29239606	4	18	theme	acid	1326:1329	arg1	β-glucosidase					1298:1310	β-glucosidase	1298:1310	β-glucosidase	1298:1310	The further ITC and docking simulations suggested the bindings of MEL-A toward β-glucosidase were driven by weak hydrophobic interactions happened between the amino acid residues of β-glucosidase and the fatty acid residues of MEL-A, in addition to hydrogen bonds between amino acids and hydroxyl in glycosyl residues of this biosurfactant.
29239606	2	19	theme	enzyme	599:604	arg1	activity					606:613	the enzyme activity	595:613	the enzyme activity	595:613	The enzyme inhibition kinetics data showed that MEL-A at a low concentration (< critical micelle concentration (CMC), 20.0 ± 5.0 μM) enhanced β-glucosidase activity, whereas it inhibited the enzyme activity at higher concentrations more than 20.0 μM, followed by a decreased Vmax and Km of β-glucosidase.
29239606	3	20	theme	high	860:863	arg1	values					865:870	high values	860:870	high values (76.6 °C, 126.3 J/g)	860:891	The thermodynamics and structural data demonstrated that the midpoint temperature (Tm) and unfolding enthalpy (ΔH) of β-glucosidase was shifted to high values (76.6 °C, 126.3 J/g) in the presence of MEL-A, and the secondary structure changes of β-glucosidase, including the increased α-helix, β-turn, or random coil contents, and a decreased β-sheet content were caused by MEL-A at a CMC concentration.
29239606	3	20	theme	high	860:863	arg1	°C					878:879	76.6 °C	873:879	76.6 °C	873:879	The thermodynamics and structural data demonstrated that the midpoint temperature (Tm) and unfolding enthalpy (ΔH) of β-glucosidase was shifted to high values (76.6 °C, 126.3 J/g) in the presence of MEL-A, and the secondary structure changes of β-glucosidase, including the increased α-helix, β-turn, or random coil contents, and a decreased β-sheet content were caused by MEL-A at a CMC concentration.
29239606	4	21	theme	amino	1388:1392	arg1	acids					1394:1398	amino acids	1388:1398	amino acids	1388:1398	The further ITC and docking simulations suggested the bindings of MEL-A toward β-glucosidase were driven by weak hydrophobic interactions happened between the amino acid residues of β-glucosidase and the fatty acid residues of MEL-A, in addition to hydrogen bonds between amino acids and hydroxyl in glycosyl residues of this biosurfactant.
29239606	2	22	theme	micelle	497:503	arg1	concentration					505:517	< critical micelle concentration	486:517	< critical micelle concentration (CMC)	486:523	The enzyme inhibition kinetics data showed that MEL-A at a low concentration (< critical micelle concentration (CMC), 20.0 ± 5.0 μM) enhanced β-glucosidase activity, whereas it inhibited the enzyme activity at higher concentrations more than 20.0 μM, followed by a decreased Vmax and Km of β-glucosidase.
29239606	2	22	theme	micelle	497:503	arg1	μM					537:538	20.0 ± 5.0 μM	526:538	20.0 ± 5.0 μM	526:538	The enzyme inhibition kinetics data showed that MEL-A at a low concentration (< critical micelle concentration (CMC), 20.0 ± 5.0 μM) enhanced β-glucosidase activity, whereas it inhibited the enzyme activity at higher concentrations more than 20.0 μM, followed by a decreased Vmax and Km of β-glucosidase.
29239606	2	22	theme	micelle	497:503	arg1	CMC					520:522	CMC	520:522	CMC	520:522	The enzyme inhibition kinetics data showed that MEL-A at a low concentration (< critical micelle concentration (CMC), 20.0 ± 5.0 μM) enhanced β-glucosidase activity, whereas it inhibited the enzyme activity at higher concentrations more than 20.0 μM, followed by a decreased Vmax and Km of β-glucosidase.
29239606	3	23	from	concentration	1101:1113	arg1	MEL-A					1086:1090	MEL-A	1086:1090	MEL-A at a CMC concentration	1086:1113	The thermodynamics and structural data demonstrated that the midpoint temperature (Tm) and unfolding enthalpy (ΔH) of β-glucosidase was shifted to high values (76.6 °C, 126.3 J/g) in the presence of MEL-A, and the secondary structure changes of β-glucosidase, including the increased α-helix, β-turn, or random coil contents, and a decreased β-sheet content were caused by MEL-A at a CMC concentration.
29239606	2	24	theme	low	467:469	arg1	concentration					471:483	a low concentration	465:483	a low concentration (< critical micelle concentration (CMC), 20.0 ± 5.0 μM)	465:539	The enzyme inhibition kinetics data showed that MEL-A at a low concentration (< critical micelle concentration (CMC), 20.0 ± 5.0 μM) enhanced β-glucosidase activity, whereas it inhibited the enzyme activity at higher concentrations more than 20.0 μM, followed by a decreased Vmax and Km of β-glucosidase.
29239606	4	25	dep	suggested	1156:1164	arg1	driven					1214:1219	driven	1214:1219	were driven by weak hydrophobic interactions happened between the amino acid residues of β-glucosidase and the fatty acid residues of MEL-A	1209:1347	The further ITC and docking simulations suggested the bindings of MEL-A toward β-glucosidase were driven by weak hydrophobic interactions happened between the amino acid residues of β-glucosidase and the fatty acid residues of MEL-A, in addition to hydrogen bonds between amino acids and hydroxyl in glycosyl residues of this biosurfactant.
29239606	2	26	theme	β-glucosidase	698:710	arg1	Vmax					683:686	Vmax	683:686	Vmax	683:686	The enzyme inhibition kinetics data showed that MEL-A at a low concentration (< critical micelle concentration (CMC), 20.0 ± 5.0 μM) enhanced β-glucosidase activity, whereas it inhibited the enzyme activity at higher concentrations more than 20.0 μM, followed by a decreased Vmax and Km of β-glucosidase.
29239606	2	26	theme	β-glucosidase	698:710	arg1	Km					692:693	Km	692:693	Km	692:693	The enzyme inhibition kinetics data showed that MEL-A at a low concentration (< critical micelle concentration (CMC), 20.0 ± 5.0 μM) enhanced β-glucosidase activity, whereas it inhibited the enzyme activity at higher concentrations more than 20.0 μM, followed by a decreased Vmax and Km of β-glucosidase.
29239606	4	27	theme	β-glucosidase	1298:1310	arg1	MEL-A					1343:1347	MEL-A	1343:1347	MEL-A	1343:1347	The further ITC and docking simulations suggested the bindings of MEL-A toward β-glucosidase were driven by weak hydrophobic interactions happened between the amino acid residues of β-glucosidase and the fatty acid residues of MEL-A, in addition to hydrogen bonds between amino acids and hydroxyl in glycosyl residues of this biosurfactant.
29239606	4	27	theme	β-glucosidase	1298:1310	arg1	residues					1331:1338	the fatty acid residues	1316:1338	the fatty acid residues of MEL-A	1316:1347	The further ITC and docking simulations suggested the bindings of MEL-A toward β-glucosidase were driven by weak hydrophobic interactions happened between the amino acid residues of β-glucosidase and the fatty acid residues of MEL-A, in addition to hydrogen bonds between amino acids and hydroxyl in glycosyl residues of this biosurfactant.
29239606	4	27	theme	β-glucosidase	1298:1310	arg1	β-glucosidase					1298:1310	β-glucosidase	1298:1310	β-glucosidase	1298:1310	The further ITC and docking simulations suggested the bindings of MEL-A toward β-glucosidase were driven by weak hydrophobic interactions happened between the amino acid residues of β-glucosidase and the fatty acid residues of MEL-A, in addition to hydrogen bonds between amino acids and hydroxyl in glycosyl residues of this biosurfactant.
29239606	4	27	theme	β-glucosidase	1298:1310	arg1	residues					1286:1293	the amino acid residues	1271:1293	the amino acid residues of β-glucosidase	1271:1310	The further ITC and docking simulations suggested the bindings of MEL-A toward β-glucosidase were driven by weak hydrophobic interactions happened between the amino acid residues of β-glucosidase and the fatty acid residues of MEL-A, in addition to hydrogen bonds between amino acids and hydroxyl in glycosyl residues of this biosurfactant.
29239606	3	28	theme	increased	987:995	arg1	α-helix					997:1003	the increased α-helix	983:1003	the increased α-helix	983:1003	The thermodynamics and structural data demonstrated that the midpoint temperature (Tm) and unfolding enthalpy (ΔH) of β-glucosidase was shifted to high values (76.6 °C, 126.3 J/g) in the presence of MEL-A, and the secondary structure changes of β-glucosidase, including the increased α-helix, β-turn, or random coil contents, and a decreased β-sheet content were caused by MEL-A at a CMC concentration.
29239606	1	29	theme	β-glucosidase	187:199	arg1	activity					201:208	β-glucosidase activity	187:208	β-glucosidase activity	187:208	In this work, the influences of a biosurfactant, mannosylerythritol lipids-A (MEL-A) toward β-glucosidase activity and their molecular interactions were studied by using differential scanning calorimetry (DSC), circular dichroism spectroscopy (CD), isothermal titration calorimetry (ITC), and docking simulation.
29239606	0	30	theme	Biosurfactant-Protein	0:20	arg1	Interaction					22:32	Biosurfactant-Protein Interaction	0:32	Biosurfactant-Protein Interaction: Influences of Mannosylerythritol Lipids-A on β-Glucosidase.	0:93	Biosurfactant-Protein Interaction: Influences of Mannosylerythritol Lipids-A on β-Glucosidase.
29239606	4	31	from	acids	1394:1398	arg1	residues					1425:1432	glycosyl residues	1416:1432	glycosyl residues of this biosurfactant	1416:1454	The further ITC and docking simulations suggested the bindings of MEL-A toward β-glucosidase were driven by weak hydrophobic interactions happened between the amino acid residues of β-glucosidase and the fatty acid residues of MEL-A, in addition to hydrogen bonds between amino acids and hydroxyl in glycosyl residues of this biosurfactant.
29239606	4	31	from	acids	1394:1398	arg1	biosurfactant					1442:1454	this biosurfactant	1437:1454	this biosurfactant	1437:1454	The further ITC and docking simulations suggested the bindings of MEL-A toward β-glucosidase were driven by weak hydrophobic interactions happened between the amino acid residues of β-glucosidase and the fatty acid residues of MEL-A, in addition to hydrogen bonds between amino acids and hydroxyl in glycosyl residues of this biosurfactant.
29239606	3	32	theme	secondary	927:935	arg1	α-helix					997:1003	the increased α-helix	983:1003	the increased α-helix	983:1003	The thermodynamics and structural data demonstrated that the midpoint temperature (Tm) and unfolding enthalpy (ΔH) of β-glucosidase was shifted to high values (76.6 °C, 126.3 J/g) in the presence of MEL-A, and the secondary structure changes of β-glucosidase, including the increased α-helix, β-turn, or random coil contents, and a decreased β-sheet content were caused by MEL-A at a CMC concentration.
29239606	3	32	theme	secondary	927:935	arg1	changes					947:953	the secondary structure changes	923:953	the secondary structure changes	923:953	The thermodynamics and structural data demonstrated that the midpoint temperature (Tm) and unfolding enthalpy (ΔH) of β-glucosidase was shifted to high values (76.6 °C, 126.3 J/g) in the presence of MEL-A, and the secondary structure changes of β-glucosidase, including the increased α-helix, β-turn, or random coil contents, and a decreased β-sheet content were caused by MEL-A at a CMC concentration.
29239606	3	32	theme	secondary	927:935	arg1	contents					1029:1036	random coil contents	1017:1036	random coil contents	1017:1036	The thermodynamics and structural data demonstrated that the midpoint temperature (Tm) and unfolding enthalpy (ΔH) of β-glucosidase was shifted to high values (76.6 °C, 126.3 J/g) in the presence of MEL-A, and the secondary structure changes of β-glucosidase, including the increased α-helix, β-turn, or random coil contents, and a decreased β-sheet content were caused by MEL-A at a CMC concentration.
29239606	3	32	theme	secondary	927:935	arg1	β-turn					1006:1011	β-turn	1006:1011	β-turn	1006:1011	The thermodynamics and structural data demonstrated that the midpoint temperature (Tm) and unfolding enthalpy (ΔH) of β-glucosidase was shifted to high values (76.6 °C, 126.3 J/g) in the presence of MEL-A, and the secondary structure changes of β-glucosidase, including the increased α-helix, β-turn, or random coil contents, and a decreased β-sheet content were caused by MEL-A at a CMC concentration.
29239606	2	33	theme	±	531:531	arg1	μM					537:538	20.0 ± 5.0 μM	526:538	20.0 ± 5.0 μM	526:538	The enzyme inhibition kinetics data showed that MEL-A at a low concentration (< critical micelle concentration (CMC), 20.0 ± 5.0 μM) enhanced β-glucosidase activity, whereas it inhibited the enzyme activity at higher concentrations more than 20.0 μM, followed by a decreased Vmax and Km of β-glucosidase.
29239606	2	33	theme	±	531:531	arg1	concentration					505:517	< critical micelle concentration	486:517	< critical micelle concentration (CMC)	486:523	The enzyme inhibition kinetics data showed that MEL-A at a low concentration (< critical micelle concentration (CMC), 20.0 ± 5.0 μM) enhanced β-glucosidase activity, whereas it inhibited the enzyme activity at higher concentrations more than 20.0 μM, followed by a decreased Vmax and Km of β-glucosidase.
29239606	3	34	theme	structure	937:945	arg1	α-helix					997:1003	the increased α-helix	983:1003	the increased α-helix	983:1003	The thermodynamics and structural data demonstrated that the midpoint temperature (Tm) and unfolding enthalpy (ΔH) of β-glucosidase was shifted to high values (76.6 °C, 126.3 J/g) in the presence of MEL-A, and the secondary structure changes of β-glucosidase, including the increased α-helix, β-turn, or random coil contents, and a decreased β-sheet content were caused by MEL-A at a CMC concentration.
29239606	3	34	theme	structure	937:945	arg1	changes					947:953	the secondary structure changes	923:953	the secondary structure changes	923:953	The thermodynamics and structural data demonstrated that the midpoint temperature (Tm) and unfolding enthalpy (ΔH) of β-glucosidase was shifted to high values (76.6 °C, 126.3 J/g) in the presence of MEL-A, and the secondary structure changes of β-glucosidase, including the increased α-helix, β-turn, or random coil contents, and a decreased β-sheet content were caused by MEL-A at a CMC concentration.
29239606	3	34	theme	structure	937:945	arg1	contents					1029:1036	random coil contents	1017:1036	random coil contents	1017:1036	The thermodynamics and structural data demonstrated that the midpoint temperature (Tm) and unfolding enthalpy (ΔH) of β-glucosidase was shifted to high values (76.6 °C, 126.3 J/g) in the presence of MEL-A, and the secondary structure changes of β-glucosidase, including the increased α-helix, β-turn, or random coil contents, and a decreased β-sheet content were caused by MEL-A at a CMC concentration.
29239606	3	34	theme	structure	937:945	arg1	β-turn					1006:1011	β-turn	1006:1011	β-turn	1006:1011	The thermodynamics and structural data demonstrated that the midpoint temperature (Tm) and unfolding enthalpy (ΔH) of β-glucosidase was shifted to high values (76.6 °C, 126.3 J/g) in the presence of MEL-A, and the secondary structure changes of β-glucosidase, including the increased α-helix, β-turn, or random coil contents, and a decreased β-sheet content were caused by MEL-A at a CMC concentration.
29239606	2	35	theme	inhibition	419:428	arg1	kinetics					430:437	The enzyme inhibition kinetics	408:437	The enzyme inhibition kinetics data	408:442	The enzyme inhibition kinetics data showed that MEL-A at a low concentration (< critical micelle concentration (CMC), 20.0 ± 5.0 μM) enhanced β-glucosidase activity, whereas it inhibited the enzyme activity at higher concentrations more than 20.0 μM, followed by a decreased Vmax and Km of β-glucosidase.
29239606	3	36	theme	CMC	1097:1099	arg1	concentration					1101:1113	a CMC concentration	1095:1113	a CMC concentration	1095:1113	The thermodynamics and structural data demonstrated that the midpoint temperature (Tm) and unfolding enthalpy (ΔH) of β-glucosidase was shifted to high values (76.6 °C, 126.3 J/g) in the presence of MEL-A, and the secondary structure changes of β-glucosidase, including the increased α-helix, β-turn, or random coil contents, and a decreased β-sheet content were caused by MEL-A at a CMC concentration.
29239606	3	37	theme	coil	1024:1027	arg1	contents					1029:1036	random coil contents	1017:1036	random coil contents	1017:1036	The thermodynamics and structural data demonstrated that the midpoint temperature (Tm) and unfolding enthalpy (ΔH) of β-glucosidase was shifted to high values (76.6 °C, 126.3 J/g) in the presence of MEL-A, and the secondary structure changes of β-glucosidase, including the increased α-helix, β-turn, or random coil contents, and a decreased β-sheet content were caused by MEL-A at a CMC concentration.
29239606	4	38	theme	hydrophobic	1229:1239	arg1	interactions					1241:1252	weak hydrophobic interactions	1224:1252	weak hydrophobic interactions happened between the amino acid residues of β-glucosidase and the fatty acid residues of MEL-A	1224:1347	The further ITC and docking simulations suggested the bindings of MEL-A toward β-glucosidase were driven by weak hydrophobic interactions happened between the amino acid residues of β-glucosidase and the fatty acid residues of MEL-A, in addition to hydrogen bonds between amino acids and hydroxyl in glycosyl residues of this biosurfactant.
29239606	4	39	theme	biosurfactant	1442:1454	arg1	residues					1425:1432	glycosyl residues	1416:1432	glycosyl residues of this biosurfactant	1416:1454	The further ITC and docking simulations suggested the bindings of MEL-A toward β-glucosidase were driven by weak hydrophobic interactions happened between the amino acid residues of β-glucosidase and the fatty acid residues of MEL-A, in addition to hydrogen bonds between amino acids and hydroxyl in glycosyl residues of this biosurfactant.
29239606	4	39	theme	biosurfactant	1442:1454	arg1	biosurfactant					1442:1454	this biosurfactant	1437:1454	this biosurfactant	1437:1454	The further ITC and docking simulations suggested the bindings of MEL-A toward β-glucosidase were driven by weak hydrophobic interactions happened between the amino acid residues of β-glucosidase and the fatty acid residues of MEL-A, in addition to hydrogen bonds between amino acids and hydroxyl in glycosyl residues of this biosurfactant.
29239606	2	40	theme	enzyme	412:417	arg1	kinetics					430:437	The enzyme inhibition kinetics	408:437	The enzyme inhibition kinetics data	408:442	The enzyme inhibition kinetics data showed that MEL-A at a low concentration (< critical micelle concentration (CMC), 20.0 ± 5.0 μM) enhanced β-glucosidase activity, whereas it inhibited the enzyme activity at higher concentrations more than 20.0 μM, followed by a decreased Vmax and Km of β-glucosidase.
29239606	0	41	theme	Mannosylerythritol	49:66	arg1	Lipids-A					68:75	Mannosylerythritol Lipids-A	49:75	Mannosylerythritol Lipids-A	49:75	Biosurfactant-Protein Interaction: Influences of Mannosylerythritol Lipids-A on β-Glucosidase.
29239606	1	42	theme	circular	306:313	arg1	spectroscopy					325:336	circular dichroism spectroscopy	306:336	circular dichroism spectroscopy (CD)	306:341	In this work, the influences of a biosurfactant, mannosylerythritol lipids-A (MEL-A) toward β-glucosidase activity and their molecular interactions were studied by using differential scanning calorimetry (DSC), circular dichroism spectroscopy (CD), isothermal titration calorimetry (ITC), and docking simulation.
29239606	1	42	theme	circular	306:313	arg1	CD					339:340	CD	339:340	CD	339:340	In this work, the influences of a biosurfactant, mannosylerythritol lipids-A (MEL-A) toward β-glucosidase activity and their molecular interactions were studied by using differential scanning calorimetry (DSC), circular dichroism spectroscopy (CD), isothermal titration calorimetry (ITC), and docking simulation.
29239606	2	43	from	concentration	471:483	arg1	MEL-A					456:460	MEL-A	456:460	MEL-A at a low concentration (< critical micelle concentration (CMC), 20.0 ± 5.0 μM)	456:539	The enzyme inhibition kinetics data showed that MEL-A at a low concentration (< critical micelle concentration (CMC), 20.0 ± 5.0 μM) enhanced β-glucosidase activity, whereas it inhibited the enzyme activity at higher concentrations more than 20.0 μM, followed by a decreased Vmax and Km of β-glucosidase.
29239606	4	44	dep	bonds	1374:1378	arg1	addition					1353:1360	addition	1353:1360	addition	1353:1360	The further ITC and docking simulations suggested the bindings of MEL-A toward β-glucosidase were driven by weak hydrophobic interactions happened between the amino acid residues of β-glucosidase and the fatty acid residues of MEL-A, in addition to hydrogen bonds between amino acids and hydroxyl in glycosyl residues of this biosurfactant.
29239606	1	45	theme	molecular	220:228	arg1	interactions					230:241	their molecular interactions	214:241	their molecular interactions	214:241	In this work, the influences of a biosurfactant, mannosylerythritol lipids-A (MEL-A) toward β-glucosidase activity and their molecular interactions were studied by using differential scanning calorimetry (DSC), circular dichroism spectroscopy (CD), isothermal titration calorimetry (ITC), and docking simulation.
29239606	1	46	theme	dichroism	315:323	arg1	spectroscopy					325:336	circular dichroism spectroscopy	306:336	circular dichroism spectroscopy (CD)	306:341	In this work, the influences of a biosurfactant, mannosylerythritol lipids-A (MEL-A) toward β-glucosidase activity and their molecular interactions were studied by using differential scanning calorimetry (DSC), circular dichroism spectroscopy (CD), isothermal titration calorimetry (ITC), and docking simulation.
29239606	1	46	theme	dichroism	315:323	arg1	CD					339:340	CD	339:340	CD	339:340	In this work, the influences of a biosurfactant, mannosylerythritol lipids-A (MEL-A) toward β-glucosidase activity and their molecular interactions were studied by using differential scanning calorimetry (DSC), circular dichroism spectroscopy (CD), isothermal titration calorimetry (ITC), and docking simulation.
29239606	1	47	theme	docking	388:394	arg1	simulation					396:405	docking simulation	388:405	docking simulation	388:405	In this work, the influences of a biosurfactant, mannosylerythritol lipids-A (MEL-A) toward β-glucosidase activity and their molecular interactions were studied by using differential scanning calorimetry (DSC), circular dichroism spectroscopy (CD), isothermal titration calorimetry (ITC), and docking simulation.
29239606	4	48	theme	hydrogen	1365:1372	arg1	bonds					1374:1378	hydrogen bonds	1365:1378	hydrogen bonds between amino acids and hydroxyl in glycosyl residues of this biosurfactant	1365:1454	The further ITC and docking simulations suggested the bindings of MEL-A toward β-glucosidase were driven by weak hydrophobic interactions happened between the amino acid residues of β-glucosidase and the fatty acid residues of MEL-A, in addition to hydrogen bonds between amino acids and hydroxyl in glycosyl residues of this biosurfactant.
29239606	3	49	theme	structural	736:745	arg1	data					747:750	structural data	736:750	structural data	736:750	The thermodynamics and structural data demonstrated that the midpoint temperature (Tm) and unfolding enthalpy (ΔH) of β-glucosidase was shifted to high values (76.6 °C, 126.3 J/g) in the presence of MEL-A, and the secondary structure changes of β-glucosidase, including the increased α-helix, β-turn, or random coil contents, and a decreased β-sheet content were caused by MEL-A at a CMC concentration.
29239606	4	50	theme	MEL-A	1182:1186	arg1	bindings					1170:1177	the bindings	1166:1177	the bindings of MEL-A toward β-glucosidase	1166:1207	The further ITC and docking simulations suggested the bindings of MEL-A toward β-glucosidase were driven by weak hydrophobic interactions happened between the amino acid residues of β-glucosidase and the fatty acid residues of MEL-A, in addition to hydrogen bonds between amino acids and hydroxyl in glycosyl residues of this biosurfactant.
29239606	0	51	theme	Lipids-A	68:75	arg1	Influences					35:44	Influences	35:44	Biosurfactant-Protein Interaction: Influences of Mannosylerythritol Lipids-A on β-Glucosidase.	0:93	Biosurfactant-Protein Interaction: Influences of Mannosylerythritol Lipids-A on β-Glucosidase.
29239606	4	52	theme	acid	1281:1284	arg1	MEL-A					1343:1347	MEL-A	1343:1347	MEL-A	1343:1347	The further ITC and docking simulations suggested the bindings of MEL-A toward β-glucosidase were driven by weak hydrophobic interactions happened between the amino acid residues of β-glucosidase and the fatty acid residues of MEL-A, in addition to hydrogen bonds between amino acids and hydroxyl in glycosyl residues of this biosurfactant.
29239606	4	52	theme	acid	1281:1284	arg1	β-glucosidase					1298:1310	β-glucosidase	1298:1310	β-glucosidase	1298:1310	The further ITC and docking simulations suggested the bindings of MEL-A toward β-glucosidase were driven by weak hydrophobic interactions happened between the amino acid residues of β-glucosidase and the fatty acid residues of MEL-A, in addition to hydrogen bonds between amino acids and hydroxyl in glycosyl residues of this biosurfactant.
29239606	4	52	theme	acid	1281:1284	arg1	residues					1286:1293	the amino acid residues	1271:1293	the amino acid residues of β-glucosidase	1271:1310	The further ITC and docking simulations suggested the bindings of MEL-A toward β-glucosidase were driven by weak hydrophobic interactions happened between the amino acid residues of β-glucosidase and the fatty acid residues of MEL-A, in addition to hydrogen bonds between amino acids and hydroxyl in glycosyl residues of this biosurfactant.
29239606	2	53	dep	concentration	471:483	arg1	concentration					505:517	< critical micelle concentration	486:517	< critical micelle concentration (CMC)	486:523	The enzyme inhibition kinetics data showed that MEL-A at a low concentration (< critical micelle concentration (CMC), 20.0 ± 5.0 μM) enhanced β-glucosidase activity, whereas it inhibited the enzyme activity at higher concentrations more than 20.0 μM, followed by a decreased Vmax and Km of β-glucosidase.
29239606	2	53	dep	concentration	471:483	arg1	μM					537:538	20.0 ± 5.0 μM	526:538	20.0 ± 5.0 μM	526:538	The enzyme inhibition kinetics data showed that MEL-A at a low concentration (< critical micelle concentration (CMC), 20.0 ± 5.0 μM) enhanced β-glucosidase activity, whereas it inhibited the enzyme activity at higher concentrations more than 20.0 μM, followed by a decreased Vmax and Km of β-glucosidase.
29239606	2	53	dep	concentration	471:483	arg1	CMC					520:522	CMC	520:522	CMC	520:522	The enzyme inhibition kinetics data showed that MEL-A at a low concentration (< critical micelle concentration (CMC), 20.0 ± 5.0 μM) enhanced β-glucosidase activity, whereas it inhibited the enzyme activity at higher concentrations more than 20.0 μM, followed by a decreased Vmax and Km of β-glucosidase.
29239606	2	54	dep	kinetics	430:437	arg1	data					439:442	data	439:442	The enzyme inhibition kinetics data	408:442	The enzyme inhibition kinetics data showed that MEL-A at a low concentration (< critical micelle concentration (CMC), 20.0 ± 5.0 μM) enhanced β-glucosidase activity, whereas it inhibited the enzyme activity at higher concentrations more than 20.0 μM, followed by a decreased Vmax and Km of β-glucosidase.
29239606	4	55	theme	amino	1275:1279	arg1	MEL-A					1343:1347	MEL-A	1343:1347	MEL-A	1343:1347	The further ITC and docking simulations suggested the bindings of MEL-A toward β-glucosidase were driven by weak hydrophobic interactions happened between the amino acid residues of β-glucosidase and the fatty acid residues of MEL-A, in addition to hydrogen bonds between amino acids and hydroxyl in glycosyl residues of this biosurfactant.
29239606	4	55	theme	amino	1275:1279	arg1	β-glucosidase					1298:1310	β-glucosidase	1298:1310	β-glucosidase	1298:1310	The further ITC and docking simulations suggested the bindings of MEL-A toward β-glucosidase were driven by weak hydrophobic interactions happened between the amino acid residues of β-glucosidase and the fatty acid residues of MEL-A, in addition to hydrogen bonds between amino acids and hydroxyl in glycosyl residues of this biosurfactant.
29239606	4	55	theme	amino	1275:1279	arg1	residues					1286:1293	the amino acid residues	1271:1293	the amino acid residues of β-glucosidase	1271:1310	The further ITC and docking simulations suggested the bindings of MEL-A toward β-glucosidase were driven by weak hydrophobic interactions happened between the amino acid residues of β-glucosidase and the fatty acid residues of MEL-A, in addition to hydrogen bonds between amino acids and hydroxyl in glycosyl residues of this biosurfactant.
29239606	4	56	theme	docking	1136:1142	arg1	simulations					1144:1154	The further ITC and docking simulations	1116:1154	simulations	1144:1154	The further ITC and docking simulations suggested the bindings of MEL-A toward β-glucosidase were driven by weak hydrophobic interactions happened between the amino acid residues of β-glucosidase and the fatty acid residues of MEL-A, in addition to hydrogen bonds between amino acids and hydroxyl in glycosyl residues of this biosurfactant.
29239606	1	57	theme	biosurfactant	129:141	arg1	influences					113:122	the influences	109:122	the influences of a biosurfactant, mannosylerythritol lipids-A (MEL-A) toward β-glucosidase activity and their molecular interactions	109:241	In this work, the influences of a biosurfactant, mannosylerythritol lipids-A (MEL-A) toward β-glucosidase activity and their molecular interactions were studied by using differential scanning calorimetry (DSC), circular dichroism spectroscopy (CD), isothermal titration calorimetry (ITC), and docking simulation.
29239606	4	58	theme	MEL-A	1343:1347	arg1	MEL-A					1343:1347	MEL-A	1343:1347	MEL-A	1343:1347	The further ITC and docking simulations suggested the bindings of MEL-A toward β-glucosidase were driven by weak hydrophobic interactions happened between the amino acid residues of β-glucosidase and the fatty acid residues of MEL-A, in addition to hydrogen bonds between amino acids and hydroxyl in glycosyl residues of this biosurfactant.
29239606	4	58	theme	MEL-A	1343:1347	arg1	residues					1331:1338	the fatty acid residues	1316:1338	the fatty acid residues of MEL-A	1316:1347	The further ITC and docking simulations suggested the bindings of MEL-A toward β-glucosidase were driven by weak hydrophobic interactions happened between the amino acid residues of β-glucosidase and the fatty acid residues of MEL-A, in addition to hydrogen bonds between amino acids and hydroxyl in glycosyl residues of this biosurfactant.
29239606	4	58	theme	MEL-A	1343:1347	arg1	β-glucosidase					1298:1310	β-glucosidase	1298:1310	β-glucosidase	1298:1310	The further ITC and docking simulations suggested the bindings of MEL-A toward β-glucosidase were driven by weak hydrophobic interactions happened between the amino acid residues of β-glucosidase and the fatty acid residues of MEL-A, in addition to hydrogen bonds between amino acids and hydroxyl in glycosyl residues of this biosurfactant.
29239606	4	58	theme	MEL-A	1343:1347	arg1	residues					1286:1293	the amino acid residues	1271:1293	the amino acid residues of β-glucosidase	1271:1310	The further ITC and docking simulations suggested the bindings of MEL-A toward β-glucosidase were driven by weak hydrophobic interactions happened between the amino acid residues of β-glucosidase and the fatty acid residues of MEL-A, in addition to hydrogen bonds between amino acids and hydroxyl in glycosyl residues of this biosurfactant.
29239606	3	59	theme	random	1017:1022	arg1	contents					1029:1036	random coil contents	1017:1036	random coil contents	1017:1036	The thermodynamics and structural data demonstrated that the midpoint temperature (Tm) and unfolding enthalpy (ΔH) of β-glucosidase was shifted to high values (76.6 °C, 126.3 J/g) in the presence of MEL-A, and the secondary structure changes of β-glucosidase, including the increased α-helix, β-turn, or random coil contents, and a decreased β-sheet content were caused by MEL-A at a CMC concentration.
29239606	2	60	dep	μM	655:656	arg1	20.0					650:653	20.0	650:653	20.0	650:653	The enzyme inhibition kinetics data showed that MEL-A at a low concentration (< critical micelle concentration (CMC), 20.0 ± 5.0 μM) enhanced β-glucosidase activity, whereas it inhibited the enzyme activity at higher concentrations more than 20.0 μM, followed by a decreased Vmax and Km of β-glucosidase.
29239606	3	61	theme	midpoint	774:781	arg1	Tm					796:797	Tm	796:797	Tm	796:797	The thermodynamics and structural data demonstrated that the midpoint temperature (Tm) and unfolding enthalpy (ΔH) of β-glucosidase was shifted to high values (76.6 °C, 126.3 J/g) in the presence of MEL-A, and the secondary structure changes of β-glucosidase, including the increased α-helix, β-turn, or random coil contents, and a decreased β-sheet content were caused by MEL-A at a CMC concentration.
29239606	3	61	theme	midpoint	774:781	arg1	temperature					783:793	the midpoint temperature	770:793	the midpoint temperature (Tm)	770:798	The thermodynamics and structural data demonstrated that the midpoint temperature (Tm) and unfolding enthalpy (ΔH) of β-glucosidase was shifted to high values (76.6 °C, 126.3 J/g) in the presence of MEL-A, and the secondary structure changes of β-glucosidase, including the increased α-helix, β-turn, or random coil contents, and a decreased β-sheet content were caused by MEL-A at a CMC concentration.
29239606	2	62	theme	critical	488:495	arg1	concentration					505:517	< critical micelle concentration	486:517	< critical micelle concentration (CMC)	486:523	The enzyme inhibition kinetics data showed that MEL-A at a low concentration (< critical micelle concentration (CMC), 20.0 ± 5.0 μM) enhanced β-glucosidase activity, whereas it inhibited the enzyme activity at higher concentrations more than 20.0 μM, followed by a decreased Vmax and Km of β-glucosidase.
29239606	2	62	theme	critical	488:495	arg1	μM					537:538	20.0 ± 5.0 μM	526:538	20.0 ± 5.0 μM	526:538	The enzyme inhibition kinetics data showed that MEL-A at a low concentration (< critical micelle concentration (CMC), 20.0 ± 5.0 μM) enhanced β-glucosidase activity, whereas it inhibited the enzyme activity at higher concentrations more than 20.0 μM, followed by a decreased Vmax and Km of β-glucosidase.
29239606	2	62	theme	critical	488:495	arg1	CMC					520:522	CMC	520:522	CMC	520:522	The enzyme inhibition kinetics data showed that MEL-A at a low concentration (< critical micelle concentration (CMC), 20.0 ± 5.0 μM) enhanced β-glucosidase activity, whereas it inhibited the enzyme activity at higher concentrations more than 20.0 μM, followed by a decreased Vmax and Km of β-glucosidase.
29239606	4	63	from	hydroxyl	1404:1411	arg1	residues					1425:1432	glycosyl residues	1416:1432	glycosyl residues of this biosurfactant	1416:1454	The further ITC and docking simulations suggested the bindings of MEL-A toward β-glucosidase were driven by weak hydrophobic interactions happened between the amino acid residues of β-glucosidase and the fatty acid residues of MEL-A, in addition to hydrogen bonds between amino acids and hydroxyl in glycosyl residues of this biosurfactant.
29239606	4	63	from	hydroxyl	1404:1411	arg1	biosurfactant					1442:1454	this biosurfactant	1437:1454	this biosurfactant	1437:1454	The further ITC and docking simulations suggested the bindings of MEL-A toward β-glucosidase were driven by weak hydrophobic interactions happened between the amino acid residues of β-glucosidase and the fatty acid residues of MEL-A, in addition to hydrogen bonds between amino acids and hydroxyl in glycosyl residues of this biosurfactant.
29239606	0	64	dep	Interaction	22:32	arg1	Influences					35:44	Influences	35:44	Biosurfactant-Protein Interaction: Influences of Mannosylerythritol Lipids-A on β-Glucosidase.	0:93	Biosurfactant-Protein Interaction: Influences of Mannosylerythritol Lipids-A on β-Glucosidase.
29239606	1	65	theme	mannosylerythritol	144:161	arg1	biosurfactant					129:141	a biosurfactant	127:141	a biosurfactant	127:141	In this work, the influences of a biosurfactant, mannosylerythritol lipids-A (MEL-A) toward β-glucosidase activity and their molecular interactions were studied by using differential scanning calorimetry (DSC), circular dichroism spectroscopy (CD), isothermal titration calorimetry (ITC), and docking simulation.
29239606	1	65	theme	mannosylerythritol	144:161	arg1	lipids-A					163:170	mannosylerythritol lipids-A	144:170	mannosylerythritol lipids-A (MEL-A)	144:178	In this work, the influences of a biosurfactant, mannosylerythritol lipids-A (MEL-A) toward β-glucosidase activity and their molecular interactions were studied by using differential scanning calorimetry (DSC), circular dichroism spectroscopy (CD), isothermal titration calorimetry (ITC), and docking simulation.
29239606	1	65	theme	mannosylerythritol	144:161	arg1	MEL-A					173:177	MEL-A	173:177	MEL-A	173:177	In this work, the influences of a biosurfactant, mannosylerythritol lipids-A (MEL-A) toward β-glucosidase activity and their molecular interactions were studied by using differential scanning calorimetry (DSC), circular dichroism spectroscopy (CD), isothermal titration calorimetry (ITC), and docking simulation.
29239606	3	66	theme	unfolding	804:812	arg1	enthalpy					814:821	unfolding enthalpy	804:821	unfolding enthalpy (ΔH) of β-glucosidase	804:843	The thermodynamics and structural data demonstrated that the midpoint temperature (Tm) and unfolding enthalpy (ΔH) of β-glucosidase was shifted to high values (76.6 °C, 126.3 J/g) in the presence of MEL-A, and the secondary structure changes of β-glucosidase, including the increased α-helix, β-turn, or random coil contents, and a decreased β-sheet content were caused by MEL-A at a CMC concentration.
29239606	3	66	theme	unfolding	804:812	arg1	ΔH					824:825	ΔH	824:825	ΔH	824:825	The thermodynamics and structural data demonstrated that the midpoint temperature (Tm) and unfolding enthalpy (ΔH) of β-glucosidase was shifted to high values (76.6 °C, 126.3 J/g) in the presence of MEL-A, and the secondary structure changes of β-glucosidase, including the increased α-helix, β-turn, or random coil contents, and a decreased β-sheet content were caused by MEL-A at a CMC concentration.
29239606	2	67	theme	<	486:486	arg1	concentration					505:517	< critical micelle concentration	486:517	< critical micelle concentration (CMC)	486:523	The enzyme inhibition kinetics data showed that MEL-A at a low concentration (< critical micelle concentration (CMC), 20.0 ± 5.0 μM) enhanced β-glucosidase activity, whereas it inhibited the enzyme activity at higher concentrations more than 20.0 μM, followed by a decreased Vmax and Km of β-glucosidase.
29239606	2	67	theme	<	486:486	arg1	μM					537:538	20.0 ± 5.0 μM	526:538	20.0 ± 5.0 μM	526:538	The enzyme inhibition kinetics data showed that MEL-A at a low concentration (< critical micelle concentration (CMC), 20.0 ± 5.0 μM) enhanced β-glucosidase activity, whereas it inhibited the enzyme activity at higher concentrations more than 20.0 μM, followed by a decreased Vmax and Km of β-glucosidase.
29239606	2	67	theme	<	486:486	arg1	CMC					520:522	CMC	520:522	CMC	520:522	The enzyme inhibition kinetics data showed that MEL-A at a low concentration (< critical micelle concentration (CMC), 20.0 ± 5.0 μM) enhanced β-glucosidase activity, whereas it inhibited the enzyme activity at higher concentrations more than 20.0 μM, followed by a decreased Vmax and Km of β-glucosidase.
29239606	0	68	from	Influences	35:44	arg1	β-Glucosidase					80:92	β-Glucosidase	80:92	β-Glucosidase	80:92	Biosurfactant-Protein Interaction: Influences of Mannosylerythritol Lipids-A on β-Glucosidase.
28640663	6	0	theme	galactosylated	1057:1070	arg1	glycan					1072:1077	galactosylated glycan	1057:1077	galactosylated glycan	1057:1077	Compared with Remicade®, Flixabi® had a lower percentage of charged glycan, and Remsima® had a higher percentage of galactosylated glycan and a lower percentage of afucose plus high mannosylated glycans.
28640663	10	1	from	similar	1620:1626	arg1	PBMC					1650:1653	PBMC	1650:1653	PBMC	1650:1653	The percentage of ADCC in NK92-CD16a cells was lower with Remsima® and higher with Flixabi® compared with Remicade®, but was similar for all 3 products in PBMC.
28640663	4	2	theme	galactosylated	607:620	arg1	glycan					622:627	galactosylated glycan	607:627	galactosylated glycan	607:627	We assessed binding to tumor necrosis factor in a fluorescence competitive binding assay; potency in a luciferase reporter gene assay; percentages of galactosylated glycan, afucose plus high mannosylated glycans, and charged glycan; FcγRIIIa (CD16) binding (assessed by 3 methods); and antibody-dependent cell-mediated cytotoxicity (ADCC) in the NK92-CD16a cell line and in peripheral blood mononuclear cells (PBMC).
28640663	3	3	dep	attributes	305:314	arg1	activity					342:349	biological activity	331:349	biological activity	331:349	Therefore, critical quality attributes (structure and biological activity) of Remicade® and of 2 biosimilar products (Flixabi®/Renflexis® and Remsima®/Inflectra®) were determined.
28640663	3	3	dep	attributes	305:314	arg1	attributes					305:314	critical quality attributes	288:314	critical quality attributes (structure and biological activity) of Remicade® and of 2 biosimilar products (Flixabi®/Renflexis® and Remsima®/Inflectra®)	288:438	Therefore, critical quality attributes (structure and biological activity) of Remicade® and of 2 biosimilar products (Flixabi®/Renflexis® and Remsima®/Inflectra®) were determined.
28640663	3	3	dep	attributes	305:314	arg1	structure					317:325	structure	317:325	structure	317:325	Therefore, critical quality attributes (structure and biological activity) of Remicade® and of 2 biosimilar products (Flixabi®/Renflexis® and Remsima®/Inflectra®) were determined.
28640663	2	4	from	differences	210:220	arg1	attributes					242:251	critical quality attributes	225:251	critical quality attributes	225:251	Manufacturing differences may lead to differences in critical quality attributes, which affect efficacy.
28640663	4	5	theme	charged	674:680	arg1	glycan					682:687	charged glycan	674:687	charged glycan	674:687	We assessed binding to tumor necrosis factor in a fluorescence competitive binding assay; potency in a luciferase reporter gene assay; percentages of galactosylated glycan, afucose plus high mannosylated glycans, and charged glycan; FcγRIIIa (CD16) binding (assessed by 3 methods); and antibody-dependent cell-mediated cytotoxicity (ADCC) in the NK92-CD16a cell line and in peripheral blood mononuclear cells (PBMC).
28640663	4	6	gly	mannosylated	648:659	arg1	glycans					661:667	high mannosylated glycans	643:667	high mannosylated glycans	643:667	We assessed binding to tumor necrosis factor in a fluorescence competitive binding assay; potency in a luciferase reporter gene assay; percentages of galactosylated glycan, afucose plus high mannosylated glycans, and charged glycan; FcγRIIIa (CD16) binding (assessed by 3 methods); and antibody-dependent cell-mediated cytotoxicity (ADCC) in the NK92-CD16a cell line and in peripheral blood mononuclear cells (PBMC).
28640663	4	7	theme	blood	842:846	arg1	cells					860:864	peripheral blood mononuclear cells	831:864	peripheral blood mononuclear cells (PBMC)	831:871	We assessed binding to tumor necrosis factor in a fluorescence competitive binding assay; potency in a luciferase reporter gene assay; percentages of galactosylated glycan, afucose plus high mannosylated glycans, and charged glycan; FcγRIIIa (CD16) binding (assessed by 3 methods); and antibody-dependent cell-mediated cytotoxicity (ADCC) in the NK92-CD16a cell line and in peripheral blood mononuclear cells (PBMC).
28640663	4	7	theme	blood	842:846	arg1	PBMC					867:870	PBMC	867:870	PBMC	867:870	We assessed binding to tumor necrosis factor in a fluorescence competitive binding assay; potency in a luciferase reporter gene assay; percentages of galactosylated glycan, afucose plus high mannosylated glycans, and charged glycan; FcγRIIIa (CD16) binding (assessed by 3 methods); and antibody-dependent cell-mediated cytotoxicity (ADCC) in the NK92-CD16a cell line and in peripheral blood mononuclear cells (PBMC).
28640663	6	8	theme	mannosylated	1123:1134	arg1	glycans					1136:1142	high mannosylated glycans	1118:1142	high mannosylated glycans	1118:1142	Compared with Remicade®, Flixabi® had a lower percentage of charged glycan, and Remsima® had a higher percentage of galactosylated glycan and a lower percentage of afucose plus high mannosylated glycans.
28640663	6	9	gly	mannosylated	1123:1134	arg1	glycans					1136:1142	high mannosylated glycans	1118:1142	high mannosylated glycans	1118:1142	Compared with Remicade®, Flixabi® had a lower percentage of charged glycan, and Remsima® had a higher percentage of galactosylated glycan and a lower percentage of afucose plus high mannosylated glycans.
28640663	10	10	from	PBMC	1650:1653	arg1	similar					1620:1626	similar	1620:1626	similar	1620:1626	The percentage of ADCC in NK92-CD16a cells was lower with Remsima® and higher with Flixabi® compared with Remicade®, but was similar for all 3 products in PBMC.
28640663	10	10	from	PBMC	1650:1653	arg1	lower					1542:1546	lower	1542:1546	lower	1542:1546	The percentage of ADCC in NK92-CD16a cells was lower with Remsima® and higher with Flixabi® compared with Remicade®, but was similar for all 3 products in PBMC.
28640663	10	10	from	PBMC	1650:1653	arg1	percentage					1499:1508	The percentage	1495:1508	The percentage of ADCC in NK92-CD16a cells	1495:1536	The percentage of ADCC in NK92-CD16a cells was lower with Remsima® and higher with Flixabi® compared with Remicade®, but was similar for all 3 products in PBMC.
28640663	4	11	theme	mannosylated	648:659	arg1	glycans					661:667	high mannosylated glycans	643:667	high mannosylated glycans	643:667	We assessed binding to tumor necrosis factor in a fluorescence competitive binding assay; potency in a luciferase reporter gene assay; percentages of galactosylated glycan, afucose plus high mannosylated glycans, and charged glycan; FcγRIIIa (CD16) binding (assessed by 3 methods); and antibody-dependent cell-mediated cytotoxicity (ADCC) in the NK92-CD16a cell line and in peripheral blood mononuclear cells (PBMC).
28640663	10	12	theme	NK92-CD16a	1521:1530	arg1	cells					1532:1536	NK92-CD16a cells	1521:1536	NK92-CD16a cells	1521:1536	The percentage of ADCC in NK92-CD16a cells was lower with Remsima® and higher with Flixabi® compared with Remicade®, but was similar for all 3 products in PBMC.
28640663	11	13	theme	NK92-CD16a	1707:1716	arg1	cells					1718:1722	NK92-CD16a cells	1707:1722	NK92-CD16a cells	1707:1722	Surface expression of CD16 was 5.7-fold greater on NK92-CD16a cells than on PBMC.
28640663	9	14	theme	FcγRIIIa	1433:1440	arg1	binding					1442:1448	FcγRIIIa binding	1433:1448	FcγRIIIa binding	1433:1448	The results of all 3 methods used in this study indicated that FcγRIIIa binding was lower with Remsima® than with Remicade®.
28640663	2	15	theme	quality	234:240	arg1	attributes					242:251	critical quality attributes	225:251	critical quality attributes	225:251	Manufacturing differences may lead to differences in critical quality attributes, which affect efficacy.
28640663	5	16	theme	Fab-related	889:899	arg1	activity					901:908	Fab-related activity	889:908	Fab-related activity	889:908	The results of Fab-related activity were similar for all products.
28640663	3	17	theme	quality	297:303	arg1	activity					342:349	biological activity	331:349	biological activity	331:349	Therefore, critical quality attributes (structure and biological activity) of Remicade® and of 2 biosimilar products (Flixabi®/Renflexis® and Remsima®/Inflectra®) were determined.
28640663	3	17	theme	quality	297:303	arg1	attributes					305:314	critical quality attributes	288:314	critical quality attributes (structure and biological activity) of Remicade® and of 2 biosimilar products (Flixabi®/Renflexis® and Remsima®/Inflectra®)	288:438	Therefore, critical quality attributes (structure and biological activity) of Remicade® and of 2 biosimilar products (Flixabi®/Renflexis® and Remsima®/Inflectra®) were determined.
28640663	3	17	theme	quality	297:303	arg1	structure					317:325	structure	317:325	structure	317:325	Therefore, critical quality attributes (structure and biological activity) of Remicade® and of 2 biosimilar products (Flixabi®/Renflexis® and Remsima®/Inflectra®) were determined.
28640663	11	18	theme	Surface	1656:1662	arg1	expression					1664:1673	Surface expression	1656:1673	Surface expression of CD16	1656:1681	Surface expression of CD16 was 5.7-fold greater on NK92-CD16a cells than on PBMC.
28640663	7	19	theme	cell	1201:1204	arg1	line					1206:1209	a Sp2/0 cell line	1193:1209	a Sp2/0 cell line	1193:1209	Whereas Remsima® and Remicade® are expressed in a Sp2/0 cell line, Flixabi® is expressed in a CHO cell line.
28640663	1	20	theme	product	144:150	arg1	quality					152:158	product quality	144:158	product quality	144:158	As biosimilars enter the market, comparisons of product quality are needed.
28640663	10	21	theme	ADCC	1513:1516	arg1	similar					1620:1626	similar	1620:1626	similar	1620:1626	The percentage of ADCC in NK92-CD16a cells was lower with Remsima® and higher with Flixabi® compared with Remicade®, but was similar for all 3 products in PBMC.
28640663	10	21	theme	ADCC	1513:1516	arg1	lower					1542:1546	lower	1542:1546	lower	1542:1546	The percentage of ADCC in NK92-CD16a cells was lower with Remsima® and higher with Flixabi® compared with Remicade®, but was similar for all 3 products in PBMC.
28640663	10	21	theme	ADCC	1513:1516	arg1	percentage					1499:1508	The percentage	1495:1508	The percentage of ADCC in NK92-CD16a cells	1495:1536	The percentage of ADCC in NK92-CD16a cells was lower with Remsima® and higher with Flixabi® compared with Remicade®, but was similar for all 3 products in PBMC.
28640663	4	22	theme	fluorescence	507:518	arg1	assay					540:544	a fluorescence competitive binding assay	505:544	a fluorescence competitive binding assay	505:544	We assessed binding to tumor necrosis factor in a fluorescence competitive binding assay; potency in a luciferase reporter gene assay; percentages of galactosylated glycan, afucose plus high mannosylated glycans, and charged glycan; FcγRIIIa (CD16) binding (assessed by 3 methods); and antibody-dependent cell-mediated cytotoxicity (ADCC) in the NK92-CD16a cell line and in peripheral blood mononuclear cells (PBMC).
28640663	8	23	theme	galactosylated	1279:1292	arg1	glycans					1294:1300	galactosylated glycans	1279:1300	galactosylated glycans from the 3 products	1279:1320	Despite this difference, galactosylated glycans from the 3 products were not correlated with the expression system.
28640663	4	24	from	cytotoxicity	776:787	arg1	assay					585:589	a luciferase reporter gene assay	558:589	a luciferase reporter gene assay	558:589	We assessed binding to tumor necrosis factor in a fluorescence competitive binding assay; potency in a luciferase reporter gene assay; percentages of galactosylated glycan, afucose plus high mannosylated glycans, and charged glycan; FcγRIIIa (CD16) binding (assessed by 3 methods); and antibody-dependent cell-mediated cytotoxicity (ADCC) in the NK92-CD16a cell line and in peripheral blood mononuclear cells (PBMC).
28640663	4	25	theme	binding	532:538	arg1	assay					540:544	a fluorescence competitive binding assay	505:544	a fluorescence competitive binding assay	505:544	We assessed binding to tumor necrosis factor in a fluorescence competitive binding assay; potency in a luciferase reporter gene assay; percentages of galactosylated glycan, afucose plus high mannosylated glycans, and charged glycan; FcγRIIIa (CD16) binding (assessed by 3 methods); and antibody-dependent cell-mediated cytotoxicity (ADCC) in the NK92-CD16a cell line and in peripheral blood mononuclear cells (PBMC).
28640663	3	26	dep	products	385:392	arg1	products					385:392	2 biosimilar products	372:392	2 biosimilar products (Flixabi®/Renflexis® and Remsima®/Inflectra®)	372:438	Therefore, critical quality attributes (structure and biological activity) of Remicade® and of 2 biosimilar products (Flixabi®/Renflexis® and Remsima®/Inflectra®) were determined.
28640663	3	26	dep	products	385:392	arg1	Remsima®/Inflectra®					419:437	Remsima®/Inflectra®	419:437	Remsima®/Inflectra®	419:437	Therefore, critical quality attributes (structure and biological activity) of Remicade® and of 2 biosimilar products (Flixabi®/Renflexis® and Remsima®/Inflectra®) were determined.
28640663	3	26	dep	products	385:392	arg1	Flixabi®/Renflexis®					395:413	Flixabi®/Renflexis®	395:413	Flixabi®/Renflexis®	395:413	Therefore, critical quality attributes (structure and biological activity) of Remicade® and of 2 biosimilar products (Flixabi®/Renflexis® and Remsima®/Inflectra®) were determined.
28640663	4	27	theme	antibody-dependent	743:760	arg1	ADCC					790:793	ADCC	790:793	ADCC	790:793	We assessed binding to tumor necrosis factor in a fluorescence competitive binding assay; potency in a luciferase reporter gene assay; percentages of galactosylated glycan, afucose plus high mannosylated glycans, and charged glycan; FcγRIIIa (CD16) binding (assessed by 3 methods); and antibody-dependent cell-mediated cytotoxicity (ADCC) in the NK92-CD16a cell line and in peripheral blood mononuclear cells (PBMC).
28640663	4	27	theme	antibody-dependent	743:760	arg1	cytotoxicity					776:787	antibody-dependent cell-mediated cytotoxicity	743:787	antibody-dependent cell-mediated cytotoxicity (ADCC)	743:794	We assessed binding to tumor necrosis factor in a fluorescence competitive binding assay; potency in a luciferase reporter gene assay; percentages of galactosylated glycan, afucose plus high mannosylated glycans, and charged glycan; FcγRIIIa (CD16) binding (assessed by 3 methods); and antibody-dependent cell-mediated cytotoxicity (ADCC) in the NK92-CD16a cell line and in peripheral blood mononuclear cells (PBMC).
28640663	4	28	theme	necrosis	486:493	arg1	factor					495:500	tumor necrosis factor	480:500	tumor necrosis factor in a fluorescence competitive binding assay	480:544	We assessed binding to tumor necrosis factor in a fluorescence competitive binding assay; potency in a luciferase reporter gene assay; percentages of galactosylated glycan, afucose plus high mannosylated glycans, and charged glycan; FcγRIIIa (CD16) binding (assessed by 3 methods); and antibody-dependent cell-mediated cytotoxicity (ADCC) in the NK92-CD16a cell line and in peripheral blood mononuclear cells (PBMC).
28640663	6	29	theme	higher	1036:1041	arg1	percentage					1043:1052	a higher percentage	1034:1052	a higher percentage of galactosylated glycan	1034:1077	Compared with Remicade®, Flixabi® had a lower percentage of charged glycan, and Remsima® had a higher percentage of galactosylated glycan and a lower percentage of afucose plus high mannosylated glycans.
28640663	3	30	theme	products	385:392	arg1	activity					342:349	biological activity	331:349	biological activity	331:349	Therefore, critical quality attributes (structure and biological activity) of Remicade® and of 2 biosimilar products (Flixabi®/Renflexis® and Remsima®/Inflectra®) were determined.
28640663	3	30	theme	products	385:392	arg1	attributes					305:314	critical quality attributes	288:314	critical quality attributes (structure and biological activity) of Remicade® and of 2 biosimilar products (Flixabi®/Renflexis® and Remsima®/Inflectra®)	288:438	Therefore, critical quality attributes (structure and biological activity) of Remicade® and of 2 biosimilar products (Flixabi®/Renflexis® and Remsima®/Inflectra®) were determined.
28640663	3	30	theme	products	385:392	arg1	structure					317:325	structure	317:325	structure	317:325	Therefore, critical quality attributes (structure and biological activity) of Remicade® and of 2 biosimilar products (Flixabi®/Renflexis® and Remsima®/Inflectra®) were determined.
28640663	0	31	theme	Glycosylation	0:12	arg1	profile					14:20	Glycosylation profile	0:20	Glycosylation profile	0:20	Glycosylation profile and biological activity of Remicade® compared with Flixabi® and Remsima®.
28640663	12	32	theme	FcγRIIIa	1837:1844	arg1	binding					1846:1852	FcγRIIIa binding	1837:1852	FcγRIIIa binding	1837:1852	Combined percentages of afucosylated and high mannosylated glycans were positively correlated with FcγRIIIa binding and ADCC in NK92-CD16 cells, while no correlation was observed in PBMC.
28640663	4	33	theme	luciferase	560:569	arg1	assay					585:589	a luciferase reporter gene assay	558:589	a luciferase reporter gene assay	558:589	We assessed binding to tumor necrosis factor in a fluorescence competitive binding assay; potency in a luciferase reporter gene assay; percentages of galactosylated glycan, afucose plus high mannosylated glycans, and charged glycan; FcγRIIIa (CD16) binding (assessed by 3 methods); and antibody-dependent cell-mediated cytotoxicity (ADCC) in the NK92-CD16a cell line and in peripheral blood mononuclear cells (PBMC).
28640663	12	34	gly	afucosylated	1762:1773	arg1	glycans					1797:1803	afucosylated and high mannosylated glycans	1762:1803	afucosylated and high mannosylated glycans	1762:1803	Combined percentages of afucosylated and high mannosylated glycans were positively correlated with FcγRIIIa binding and ADCC in NK92-CD16 cells, while no correlation was observed in PBMC.
28640663	12	35	theme	mannosylated	1784:1795	arg1	glycans					1797:1803	afucosylated and high mannosylated glycans	1762:1803	afucosylated and high mannosylated glycans	1762:1803	Combined percentages of afucosylated and high mannosylated glycans were positively correlated with FcγRIIIa binding and ADCC in NK92-CD16 cells, while no correlation was observed in PBMC.
28640663	4	36	dep	binding	706:712	arg1	assessed					715:722	assessed	715:722	assessed by 3 methods	715:735	We assessed binding to tumor necrosis factor in a fluorescence competitive binding assay; potency in a luciferase reporter gene assay; percentages of galactosylated glycan, afucose plus high mannosylated glycans, and charged glycan; FcγRIIIa (CD16) binding (assessed by 3 methods); and antibody-dependent cell-mediated cytotoxicity (ADCC) in the NK92-CD16a cell line and in peripheral blood mononuclear cells (PBMC).
28640663	4	37	theme	gene	580:583	arg1	assay					585:589	a luciferase reporter gene assay	558:589	a luciferase reporter gene assay	558:589	We assessed binding to tumor necrosis factor in a fluorescence competitive binding assay; potency in a luciferase reporter gene assay; percentages of galactosylated glycan, afucose plus high mannosylated glycans, and charged glycan; FcγRIIIa (CD16) binding (assessed by 3 methods); and antibody-dependent cell-mediated cytotoxicity (ADCC) in the NK92-CD16a cell line and in peripheral blood mononuclear cells (PBMC).
28640663	2	38	theme	Manufacturing	172:184	arg1	differences					186:196	Manufacturing differences	172:196	Manufacturing differences	172:196	Manufacturing differences may lead to differences in critical quality attributes, which affect efficacy.
28640663	4	39	theme	NK92-CD16a	803:812	arg1	line					819:822	the NK92-CD16a cell line	799:822	the NK92-CD16a cell line	799:822	We assessed binding to tumor necrosis factor in a fluorescence competitive binding assay; potency in a luciferase reporter gene assay; percentages of galactosylated glycan, afucose plus high mannosylated glycans, and charged glycan; FcγRIIIa (CD16) binding (assessed by 3 methods); and antibody-dependent cell-mediated cytotoxicity (ADCC) in the NK92-CD16a cell line and in peripheral blood mononuclear cells (PBMC).
28640663	6	40	theme	glycan	1009:1014	arg1	percentage					987:996	a lower percentage	979:996	a lower percentage of charged glycan	979:1014	Compared with Remicade®, Flixabi® had a lower percentage of charged glycan, and Remsima® had a higher percentage of galactosylated glycan and a lower percentage of afucose plus high mannosylated glycans.
28640663	4	41	from	afucose	630:636	arg1	assay					585:589	a luciferase reporter gene assay	558:589	a luciferase reporter gene assay	558:589	We assessed binding to tumor necrosis factor in a fluorescence competitive binding assay; potency in a luciferase reporter gene assay; percentages of galactosylated glycan, afucose plus high mannosylated glycans, and charged glycan; FcγRIIIa (CD16) binding (assessed by 3 methods); and antibody-dependent cell-mediated cytotoxicity (ADCC) in the NK92-CD16a cell line and in peripheral blood mononuclear cells (PBMC).
28640663	12	42	located	observed	1908:1915	arg1	PBMC					1920:1923	PBMC	1920:1923	PBMC	1920:1923	Combined percentages of afucosylated and high mannosylated glycans were positively correlated with FcγRIIIa binding and ADCC in NK92-CD16 cells, while no correlation was observed in PBMC.
28640663	12	42	located	observed	1908:1915	arg2	correlation					1892:1902	no correlation	1889:1902	no correlation	1889:1902	Combined percentages of afucosylated and high mannosylated glycans were positively correlated with FcγRIIIa binding and ADCC in NK92-CD16 cells, while no correlation was observed in PBMC.
28640663	0	43	theme	Remicade®	49:57	arg1	profile					14:20	Glycosylation profile	0:20	Glycosylation profile	0:20	Glycosylation profile and biological activity of Remicade® compared with Flixabi® and Remsima®.
28640663	0	43	theme	Remicade®	49:57	arg1	activity					37:44	biological activity	26:44	biological activity	26:44	Glycosylation profile and biological activity of Remicade® compared with Flixabi® and Remsima®.
28640663	4	44	theme	competitive	520:530	arg1	assay					540:544	a fluorescence competitive binding assay	505:544	a fluorescence competitive binding assay	505:544	We assessed binding to tumor necrosis factor in a fluorescence competitive binding assay; potency in a luciferase reporter gene assay; percentages of galactosylated glycan, afucose plus high mannosylated glycans, and charged glycan; FcγRIIIa (CD16) binding (assessed by 3 methods); and antibody-dependent cell-mediated cytotoxicity (ADCC) in the NK92-CD16a cell line and in peripheral blood mononuclear cells (PBMC).
28640663	7	45	theme	CHO	1239:1241	arg1	line					1248:1251	a CHO cell line	1237:1251	a CHO cell line	1237:1251	Whereas Remsima® and Remicade® are expressed in a Sp2/0 cell line, Flixabi® is expressed in a CHO cell line.
28640663	4	46	theme	glycan	622:627	arg1	ADCC					790:793	ADCC	790:793	ADCC	790:793	We assessed binding to tumor necrosis factor in a fluorescence competitive binding assay; potency in a luciferase reporter gene assay; percentages of galactosylated glycan, afucose plus high mannosylated glycans, and charged glycan; FcγRIIIa (CD16) binding (assessed by 3 methods); and antibody-dependent cell-mediated cytotoxicity (ADCC) in the NK92-CD16a cell line and in peripheral blood mononuclear cells (PBMC).
28640663	4	46	theme	glycan	622:627	arg1	glycans					661:667	high mannosylated glycans	643:667	high mannosylated glycans	643:667	We assessed binding to tumor necrosis factor in a fluorescence competitive binding assay; potency in a luciferase reporter gene assay; percentages of galactosylated glycan, afucose plus high mannosylated glycans, and charged glycan; FcγRIIIa (CD16) binding (assessed by 3 methods); and antibody-dependent cell-mediated cytotoxicity (ADCC) in the NK92-CD16a cell line and in peripheral blood mononuclear cells (PBMC).
28640663	4	46	theme	glycan	622:627	arg1	cytotoxicity					776:787	antibody-dependent cell-mediated cytotoxicity	743:787	antibody-dependent cell-mediated cytotoxicity (ADCC)	743:794	We assessed binding to tumor necrosis factor in a fluorescence competitive binding assay; potency in a luciferase reporter gene assay; percentages of galactosylated glycan, afucose plus high mannosylated glycans, and charged glycan; FcγRIIIa (CD16) binding (assessed by 3 methods); and antibody-dependent cell-mediated cytotoxicity (ADCC) in the NK92-CD16a cell line and in peripheral blood mononuclear cells (PBMC).
28640663	4	46	theme	glycan	622:627	arg1	afucose					630:636	afucose	630:636	afucose	630:636	We assessed binding to tumor necrosis factor in a fluorescence competitive binding assay; potency in a luciferase reporter gene assay; percentages of galactosylated glycan, afucose plus high mannosylated glycans, and charged glycan; FcγRIIIa (CD16) binding (assessed by 3 methods); and antibody-dependent cell-mediated cytotoxicity (ADCC) in the NK92-CD16a cell line and in peripheral blood mononuclear cells (PBMC).
28640663	4	46	theme	glycan	622:627	arg1	potency					547:553	potency	547:553	potency in a luciferase reporter gene assay	547:589	We assessed binding to tumor necrosis factor in a fluorescence competitive binding assay; potency in a luciferase reporter gene assay; percentages of galactosylated glycan, afucose plus high mannosylated glycans, and charged glycan; FcγRIIIa (CD16) binding (assessed by 3 methods); and antibody-dependent cell-mediated cytotoxicity (ADCC) in the NK92-CD16a cell line and in peripheral blood mononuclear cells (PBMC).
28640663	4	46	theme	glycan	622:627	arg1	binding					469:475	binding	469:475	binding to tumor necrosis factor in a fluorescence competitive binding assay	469:544	We assessed binding to tumor necrosis factor in a fluorescence competitive binding assay; potency in a luciferase reporter gene assay; percentages of galactosylated glycan, afucose plus high mannosylated glycans, and charged glycan; FcγRIIIa (CD16) binding (assessed by 3 methods); and antibody-dependent cell-mediated cytotoxicity (ADCC) in the NK92-CD16a cell line and in peripheral blood mononuclear cells (PBMC).
28640663	4	46	theme	glycan	622:627	arg1	glycan					682:687	charged glycan	674:687	charged glycan	674:687	We assessed binding to tumor necrosis factor in a fluorescence competitive binding assay; potency in a luciferase reporter gene assay; percentages of galactosylated glycan, afucose plus high mannosylated glycans, and charged glycan; FcγRIIIa (CD16) binding (assessed by 3 methods); and antibody-dependent cell-mediated cytotoxicity (ADCC) in the NK92-CD16a cell line and in peripheral blood mononuclear cells (PBMC).
28640663	4	46	theme	glycan	622:627	arg1	percentages					592:602	percentages	592:602	percentages of galactosylated glycan	592:627	We assessed binding to tumor necrosis factor in a fluorescence competitive binding assay; potency in a luciferase reporter gene assay; percentages of galactosylated glycan, afucose plus high mannosylated glycans, and charged glycan; FcγRIIIa (CD16) binding (assessed by 3 methods); and antibody-dependent cell-mediated cytotoxicity (ADCC) in the NK92-CD16a cell line and in peripheral blood mononuclear cells (PBMC).
28640663	4	46	theme	glycan	622:627	arg1	binding					706:712	FcγRIIIa (CD16) binding	690:712	FcγRIIIa (CD16) binding (assessed by 3 methods)	690:736	We assessed binding to tumor necrosis factor in a fluorescence competitive binding assay; potency in a luciferase reporter gene assay; percentages of galactosylated glycan, afucose plus high mannosylated glycans, and charged glycan; FcγRIIIa (CD16) binding (assessed by 3 methods); and antibody-dependent cell-mediated cytotoxicity (ADCC) in the NK92-CD16a cell line and in peripheral blood mononuclear cells (PBMC).
28640663	4	47	from	binding	706:712	arg1	assay					585:589	a luciferase reporter gene assay	558:589	a luciferase reporter gene assay	558:589	We assessed binding to tumor necrosis factor in a fluorescence competitive binding assay; potency in a luciferase reporter gene assay; percentages of galactosylated glycan, afucose plus high mannosylated glycans, and charged glycan; FcγRIIIa (CD16) binding (assessed by 3 methods); and antibody-dependent cell-mediated cytotoxicity (ADCC) in the NK92-CD16a cell line and in peripheral blood mononuclear cells (PBMC).
28640663	10	48	from	cells	1532:1536	arg1	similar					1620:1626	similar	1620:1626	similar	1620:1626	The percentage of ADCC in NK92-CD16a cells was lower with Remsima® and higher with Flixabi® compared with Remicade®, but was similar for all 3 products in PBMC.
28640663	10	48	from	cells	1532:1536	arg1	lower					1542:1546	lower	1542:1546	lower	1542:1546	The percentage of ADCC in NK92-CD16a cells was lower with Remsima® and higher with Flixabi® compared with Remicade®, but was similar for all 3 products in PBMC.
28640663	10	48	from	cells	1532:1536	arg1	percentage					1499:1508	The percentage	1495:1508	The percentage of ADCC in NK92-CD16a cells	1495:1536	The percentage of ADCC in NK92-CD16a cells was lower with Remsima® and higher with Flixabi® compared with Remicade®, but was similar for all 3 products in PBMC.
28640663	6	49	theme	lower	981:985	arg1	percentage					987:996	a lower percentage	979:996	a lower percentage of charged glycan	979:1014	Compared with Remicade®, Flixabi® had a lower percentage of charged glycan, and Remsima® had a higher percentage of galactosylated glycan and a lower percentage of afucose plus high mannosylated glycans.
28640663	6	50	theme	glycan	1072:1077	arg1	percentage					1091:1100	a lower percentage	1083:1100	a lower percentage of afucose plus high mannosylated glycans	1083:1142	Compared with Remicade®, Flixabi® had a lower percentage of charged glycan, and Remsima® had a higher percentage of galactosylated glycan and a lower percentage of afucose plus high mannosylated glycans.
28640663	6	50	theme	glycan	1072:1077	arg1	percentage					1043:1052	a higher percentage	1034:1052	a higher percentage of galactosylated glycan	1034:1077	Compared with Remicade®, Flixabi® had a lower percentage of charged glycan, and Remsima® had a higher percentage of galactosylated glycan and a lower percentage of afucose plus high mannosylated glycans.
28640663	4	51	theme	mononuclear	848:858	arg1	cells					860:864	peripheral blood mononuclear cells	831:864	peripheral blood mononuclear cells (PBMC)	831:871	We assessed binding to tumor necrosis factor in a fluorescence competitive binding assay; potency in a luciferase reporter gene assay; percentages of galactosylated glycan, afucose plus high mannosylated glycans, and charged glycan; FcγRIIIa (CD16) binding (assessed by 3 methods); and antibody-dependent cell-mediated cytotoxicity (ADCC) in the NK92-CD16a cell line and in peripheral blood mononuclear cells (PBMC).
28640663	4	51	theme	mononuclear	848:858	arg1	PBMC					867:870	PBMC	867:870	PBMC	867:870	We assessed binding to tumor necrosis factor in a fluorescence competitive binding assay; potency in a luciferase reporter gene assay; percentages of galactosylated glycan, afucose plus high mannosylated glycans, and charged glycan; FcγRIIIa (CD16) binding (assessed by 3 methods); and antibody-dependent cell-mediated cytotoxicity (ADCC) in the NK92-CD16a cell line and in peripheral blood mononuclear cells (PBMC).
28640663	8	52	from	products	1313:1320	arg1	glycans					1294:1300	galactosylated glycans	1279:1300	galactosylated glycans from the 3 products	1279:1320	Despite this difference, galactosylated glycans from the 3 products were not correlated with the expression system.
28640663	4	53	from	glycan	682:687	arg1	assay					585:589	a luciferase reporter gene assay	558:589	a luciferase reporter gene assay	558:589	We assessed binding to tumor necrosis factor in a fluorescence competitive binding assay; potency in a luciferase reporter gene assay; percentages of galactosylated glycan, afucose plus high mannosylated glycans, and charged glycan; FcγRIIIa (CD16) binding (assessed by 3 methods); and antibody-dependent cell-mediated cytotoxicity (ADCC) in the NK92-CD16a cell line and in peripheral blood mononuclear cells (PBMC).
28640663	6	54	theme	glycans	1136:1142	arg1	percentage					1091:1100	a lower percentage	1083:1100	a lower percentage of afucose plus high mannosylated glycans	1083:1142	Compared with Remicade®, Flixabi® had a lower percentage of charged glycan, and Remsima® had a higher percentage of galactosylated glycan and a lower percentage of afucose plus high mannosylated glycans.
28640663	6	54	theme	glycans	1136:1142	arg1	percentage					1043:1052	a higher percentage	1034:1052	a higher percentage of galactosylated glycan	1034:1077	Compared with Remicade®, Flixabi® had a lower percentage of charged glycan, and Remsima® had a higher percentage of galactosylated glycan and a lower percentage of afucose plus high mannosylated glycans.
28640663	4	55	from	factor	495:500	arg1	assay					540:544	a fluorescence competitive binding assay	505:544	a fluorescence competitive binding assay	505:544	We assessed binding to tumor necrosis factor in a fluorescence competitive binding assay; potency in a luciferase reporter gene assay; percentages of galactosylated glycan, afucose plus high mannosylated glycans, and charged glycan; FcγRIIIa (CD16) binding (assessed by 3 methods); and antibody-dependent cell-mediated cytotoxicity (ADCC) in the NK92-CD16a cell line and in peripheral blood mononuclear cells (PBMC).
28640663	9	56	theme	methods	1391:1397	arg1	results					1374:1380	The results	1370:1380	The results of all 3 methods used in this study	1370:1416	The results of all 3 methods used in this study indicated that FcγRIIIa binding was lower with Remsima® than with Remicade®.
28640663	4	57	theme	peripheral	831:840	arg1	cells					860:864	peripheral blood mononuclear cells	831:864	peripheral blood mononuclear cells (PBMC)	831:871	We assessed binding to tumor necrosis factor in a fluorescence competitive binding assay; potency in a luciferase reporter gene assay; percentages of galactosylated glycan, afucose plus high mannosylated glycans, and charged glycan; FcγRIIIa (CD16) binding (assessed by 3 methods); and antibody-dependent cell-mediated cytotoxicity (ADCC) in the NK92-CD16a cell line and in peripheral blood mononuclear cells (PBMC).
28640663	4	57	theme	peripheral	831:840	arg1	PBMC					867:870	PBMC	867:870	PBMC	867:870	We assessed binding to tumor necrosis factor in a fluorescence competitive binding assay; potency in a luciferase reporter gene assay; percentages of galactosylated glycan, afucose plus high mannosylated glycans, and charged glycan; FcγRIIIa (CD16) binding (assessed by 3 methods); and antibody-dependent cell-mediated cytotoxicity (ADCC) in the NK92-CD16a cell line and in peripheral blood mononuclear cells (PBMC).
28640663	6	58	theme	high	1118:1121	arg1	glycans					1136:1142	high mannosylated glycans	1118:1142	high mannosylated glycans	1118:1142	Compared with Remicade®, Flixabi® had a lower percentage of charged glycan, and Remsima® had a higher percentage of galactosylated glycan and a lower percentage of afucose plus high mannosylated glycans.
28640663	4	59	theme	cell	814:817	arg1	line					819:822	the NK92-CD16a cell line	799:822	the NK92-CD16a cell line	799:822	We assessed binding to tumor necrosis factor in a fluorescence competitive binding assay; potency in a luciferase reporter gene assay; percentages of galactosylated glycan, afucose plus high mannosylated glycans, and charged glycan; FcγRIIIa (CD16) binding (assessed by 3 methods); and antibody-dependent cell-mediated cytotoxicity (ADCC) in the NK92-CD16a cell line and in peripheral blood mononuclear cells (PBMC).
28640663	4	60	from	glycans	661:667	arg1	assay					585:589	a luciferase reporter gene assay	558:589	a luciferase reporter gene assay	558:589	We assessed binding to tumor necrosis factor in a fluorescence competitive binding assay; potency in a luciferase reporter gene assay; percentages of galactosylated glycan, afucose plus high mannosylated glycans, and charged glycan; FcγRIIIa (CD16) binding (assessed by 3 methods); and antibody-dependent cell-mediated cytotoxicity (ADCC) in the NK92-CD16a cell line and in peripheral blood mononuclear cells (PBMC).
28640663	3	61	theme	critical	288:295	arg1	activity					342:349	biological activity	331:349	biological activity	331:349	Therefore, critical quality attributes (structure and biological activity) of Remicade® and of 2 biosimilar products (Flixabi®/Renflexis® and Remsima®/Inflectra®) were determined.
28640663	3	61	theme	critical	288:295	arg1	attributes					305:314	critical quality attributes	288:314	critical quality attributes (structure and biological activity) of Remicade® and of 2 biosimilar products (Flixabi®/Renflexis® and Remsima®/Inflectra®)	288:438	Therefore, critical quality attributes (structure and biological activity) of Remicade® and of 2 biosimilar products (Flixabi®/Renflexis® and Remsima®/Inflectra®) were determined.
28640663	3	61	theme	critical	288:295	arg1	structure					317:325	structure	317:325	structure	317:325	Therefore, critical quality attributes (structure and biological activity) of Remicade® and of 2 biosimilar products (Flixabi®/Renflexis® and Remsima®/Inflectra®) were determined.
28640663	6	62	theme	afucose	1105:1111	arg1	percentage					1091:1100	a lower percentage	1083:1100	a lower percentage of afucose plus high mannosylated glycans	1083:1142	Compared with Remicade®, Flixabi® had a lower percentage of charged glycan, and Remsima® had a higher percentage of galactosylated glycan and a lower percentage of afucose plus high mannosylated glycans.
28640663	6	62	theme	afucose	1105:1111	arg1	percentage					1043:1052	a higher percentage	1034:1052	a higher percentage of galactosylated glycan	1034:1077	Compared with Remicade®, Flixabi® had a lower percentage of charged glycan, and Remsima® had a higher percentage of galactosylated glycan and a lower percentage of afucose plus high mannosylated glycans.
28640663	4	63	theme	high	643:646	arg1	glycans					661:667	high mannosylated glycans	643:667	high mannosylated glycans	643:667	We assessed binding to tumor necrosis factor in a fluorescence competitive binding assay; potency in a luciferase reporter gene assay; percentages of galactosylated glycan, afucose plus high mannosylated glycans, and charged glycan; FcγRIIIa (CD16) binding (assessed by 3 methods); and antibody-dependent cell-mediated cytotoxicity (ADCC) in the NK92-CD16a cell line and in peripheral blood mononuclear cells (PBMC).
28640663	4	64	from	binding	469:475	arg1	assay					585:589	a luciferase reporter gene assay	558:589	a luciferase reporter gene assay	558:589	We assessed binding to tumor necrosis factor in a fluorescence competitive binding assay; potency in a luciferase reporter gene assay; percentages of galactosylated glycan, afucose plus high mannosylated glycans, and charged glycan; FcγRIIIa (CD16) binding (assessed by 3 methods); and antibody-dependent cell-mediated cytotoxicity (ADCC) in the NK92-CD16a cell line and in peripheral blood mononuclear cells (PBMC).
28640663	4	65	theme	tumor	480:484	arg1	factor					495:500	tumor necrosis factor	480:500	tumor necrosis factor in a fluorescence competitive binding assay	480:544	We assessed binding to tumor necrosis factor in a fluorescence competitive binding assay; potency in a luciferase reporter gene assay; percentages of galactosylated glycan, afucose plus high mannosylated glycans, and charged glycan; FcγRIIIa (CD16) binding (assessed by 3 methods); and antibody-dependent cell-mediated cytotoxicity (ADCC) in the NK92-CD16a cell line and in peripheral blood mononuclear cells (PBMC).
28640663	2	66	theme	critical	225:232	arg1	attributes					242:251	critical quality attributes	225:251	critical quality attributes	225:251	Manufacturing differences may lead to differences in critical quality attributes, which affect efficacy.
28640663	11	67	dep	5.7-fold	1687:1694	arg1	greater					1696:1702	greater	1696:1702	greater	1696:1702	Surface expression of CD16 was 5.7-fold greater on NK92-CD16a cells than on PBMC.
28640663	5	68	theme	activity	901:908	arg1	similar					915:921	similar	915:921	similar	915:921	The results of Fab-related activity were similar for all products.
28640663	5	68	theme	activity	901:908	arg1	results					878:884	The results	874:884	The results of Fab-related activity	874:908	The results of Fab-related activity were similar for all products.
28640663	6	69	theme	charged	1001:1007	arg1	glycan					1009:1014	charged glycan	1001:1014	charged glycan	1001:1014	Compared with Remicade®, Flixabi® had a lower percentage of charged glycan, and Remsima® had a higher percentage of galactosylated glycan and a lower percentage of afucose plus high mannosylated glycans.
28640663	11	70	theme	CD16	1678:1681	arg1	expression					1664:1673	Surface expression	1656:1673	Surface expression of CD16	1656:1681	Surface expression of CD16 was 5.7-fold greater on NK92-CD16a cells than on PBMC.
28640663	7	71	theme	Sp2/0	1195:1199	arg1	line					1206:1209	a Sp2/0 cell line	1193:1209	a Sp2/0 cell line	1193:1209	Whereas Remsima® and Remicade® are expressed in a Sp2/0 cell line, Flixabi® is expressed in a CHO cell line.
28640663	12	72	from	ADCC	1858:1861	arg1	cells					1876:1880	NK92-CD16 cells	1866:1880	NK92-CD16 cells	1866:1880	Combined percentages of afucosylated and high mannosylated glycans were positively correlated with FcγRIIIa binding and ADCC in NK92-CD16 cells, while no correlation was observed in PBMC.
28640663	6	73	contain	had	1030:1032	arg2	percentage					1043:1052	a higher percentage	1034:1052	a higher percentage of galactosylated glycan	1034:1077	Compared with Remicade®, Flixabi® had a lower percentage of charged glycan, and Remsima® had a higher percentage of galactosylated glycan and a lower percentage of afucose plus high mannosylated glycans.
28640663	6	73	contain	had	1030:1032	arg1	Remsima®					1021:1028	Remsima®	1021:1028	Remsima®	1021:1028	Compared with Remicade®, Flixabi® had a lower percentage of charged glycan, and Remsima® had a higher percentage of galactosylated glycan and a lower percentage of afucose plus high mannosylated glycans.
28640663	6	73	contain	had	1030:1032	arg2	percentage					1091:1100	a lower percentage	1083:1100	a lower percentage of afucose plus high mannosylated glycans	1083:1142	Compared with Remicade®, Flixabi® had a lower percentage of charged glycan, and Remsima® had a higher percentage of galactosylated glycan and a lower percentage of afucose plus high mannosylated glycans.
28640663	4	74	theme	FcγRIIIa	690:697	arg1	binding					706:712	FcγRIIIa (CD16) binding	690:712	FcγRIIIa (CD16) binding (assessed by 3 methods)	690:736	We assessed binding to tumor necrosis factor in a fluorescence competitive binding assay; potency in a luciferase reporter gene assay; percentages of galactosylated glycan, afucose plus high mannosylated glycans, and charged glycan; FcγRIIIa (CD16) binding (assessed by 3 methods); and antibody-dependent cell-mediated cytotoxicity (ADCC) in the NK92-CD16a cell line and in peripheral blood mononuclear cells (PBMC).
28640663	1	75	theme	quality	152:158	arg1	comparisons					129:139	comparisons	129:139	comparisons of product quality	129:158	As biosimilars enter the market, comparisons of product quality are needed.
28640663	12	76	from	binding	1846:1852	arg1	cells					1876:1880	NK92-CD16 cells	1866:1880	NK92-CD16 cells	1866:1880	Combined percentages of afucosylated and high mannosylated glycans were positively correlated with FcγRIIIa binding and ADCC in NK92-CD16 cells, while no correlation was observed in PBMC.
28640663	3	77	theme	biological	331:340	arg1	activity					342:349	biological activity	331:349	biological activity	331:349	Therefore, critical quality attributes (structure and biological activity) of Remicade® and of 2 biosimilar products (Flixabi®/Renflexis® and Remsima®/Inflectra®) were determined.
28640663	3	77	theme	biological	331:340	arg1	attributes					305:314	critical quality attributes	288:314	critical quality attributes (structure and biological activity) of Remicade® and of 2 biosimilar products (Flixabi®/Renflexis® and Remsima®/Inflectra®)	288:438	Therefore, critical quality attributes (structure and biological activity) of Remicade® and of 2 biosimilar products (Flixabi®/Renflexis® and Remsima®/Inflectra®) were determined.
28640663	4	78	theme	CD16	700:703	arg1	binding					706:712	FcγRIIIa (CD16) binding	690:712	FcγRIIIa (CD16) binding (assessed by 3 methods)	690:736	We assessed binding to tumor necrosis factor in a fluorescence competitive binding assay; potency in a luciferase reporter gene assay; percentages of galactosylated glycan, afucose plus high mannosylated glycans, and charged glycan; FcγRIIIa (CD16) binding (assessed by 3 methods); and antibody-dependent cell-mediated cytotoxicity (ADCC) in the NK92-CD16a cell line and in peripheral blood mononuclear cells (PBMC).
28640663	12	79	theme	NK92-CD16	1866:1874	arg1	cells					1876:1880	NK92-CD16 cells	1866:1880	NK92-CD16 cells	1866:1880	Combined percentages of afucosylated and high mannosylated glycans were positively correlated with FcγRIIIa binding and ADCC in NK92-CD16 cells, while no correlation was observed in PBMC.
28640663	12	80	gly	mannosylated	1784:1795	arg1	glycans					1797:1803	afucosylated and high mannosylated glycans	1762:1803	afucosylated and high mannosylated glycans	1762:1803	Combined percentages of afucosylated and high mannosylated glycans were positively correlated with FcγRIIIa binding and ADCC in NK92-CD16 cells, while no correlation was observed in PBMC.
28640663	4	81	from	percentages	592:602	arg1	assay					585:589	a luciferase reporter gene assay	558:589	a luciferase reporter gene assay	558:589	We assessed binding to tumor necrosis factor in a fluorescence competitive binding assay; potency in a luciferase reporter gene assay; percentages of galactosylated glycan, afucose plus high mannosylated glycans, and charged glycan; FcγRIIIa (CD16) binding (assessed by 3 methods); and antibody-dependent cell-mediated cytotoxicity (ADCC) in the NK92-CD16a cell line and in peripheral blood mononuclear cells (PBMC).
28640663	3	82	theme	Remicade®	355:363	arg1	activity					342:349	biological activity	331:349	biological activity	331:349	Therefore, critical quality attributes (structure and biological activity) of Remicade® and of 2 biosimilar products (Flixabi®/Renflexis® and Remsima®/Inflectra®) were determined.
28640663	3	82	theme	Remicade®	355:363	arg1	attributes					305:314	critical quality attributes	288:314	critical quality attributes (structure and biological activity) of Remicade® and of 2 biosimilar products (Flixabi®/Renflexis® and Remsima®/Inflectra®)	288:438	Therefore, critical quality attributes (structure and biological activity) of Remicade® and of 2 biosimilar products (Flixabi®/Renflexis® and Remsima®/Inflectra®) were determined.
28640663	3	82	theme	Remicade®	355:363	arg1	structure					317:325	structure	317:325	structure	317:325	Therefore, critical quality attributes (structure and biological activity) of Remicade® and of 2 biosimilar products (Flixabi®/Renflexis® and Remsima®/Inflectra®) were determined.
28640663	4	83	theme	cell-mediated	762:774	arg1	ADCC					790:793	ADCC	790:793	ADCC	790:793	We assessed binding to tumor necrosis factor in a fluorescence competitive binding assay; potency in a luciferase reporter gene assay; percentages of galactosylated glycan, afucose plus high mannosylated glycans, and charged glycan; FcγRIIIa (CD16) binding (assessed by 3 methods); and antibody-dependent cell-mediated cytotoxicity (ADCC) in the NK92-CD16a cell line and in peripheral blood mononuclear cells (PBMC).
28640663	4	83	theme	cell-mediated	762:774	arg1	cytotoxicity					776:787	antibody-dependent cell-mediated cytotoxicity	743:787	antibody-dependent cell-mediated cytotoxicity (ADCC)	743:794	We assessed binding to tumor necrosis factor in a fluorescence competitive binding assay; potency in a luciferase reporter gene assay; percentages of galactosylated glycan, afucose plus high mannosylated glycans, and charged glycan; FcγRIIIa (CD16) binding (assessed by 3 methods); and antibody-dependent cell-mediated cytotoxicity (ADCC) in the NK92-CD16a cell line and in peripheral blood mononuclear cells (PBMC).
28640663	4	84	theme	reporter	571:578	arg1	assay					585:589	a luciferase reporter gene assay	558:589	a luciferase reporter gene assay	558:589	We assessed binding to tumor necrosis factor in a fluorescence competitive binding assay; potency in a luciferase reporter gene assay; percentages of galactosylated glycan, afucose plus high mannosylated glycans, and charged glycan; FcγRIIIa (CD16) binding (assessed by 3 methods); and antibody-dependent cell-mediated cytotoxicity (ADCC) in the NK92-CD16a cell line and in peripheral blood mononuclear cells (PBMC).
28640663	0	85	theme	biological	26:35	arg1	activity					37:44	biological activity	26:44	biological activity	26:44	Glycosylation profile and biological activity of Remicade® compared with Flixabi® and Remsima®.
28640663	12	86	theme	glycans	1797:1803	arg1	percentages					1747:1757	Combined percentages	1738:1757	Combined percentages of afucosylated and high mannosylated glycans	1738:1803	Combined percentages of afucosylated and high mannosylated glycans were positively correlated with FcγRIIIa binding and ADCC in NK92-CD16 cells, while no correlation was observed in PBMC.
28640663	3	87	theme	biosimilar	374:383	arg1	products					385:392	2 biosimilar products	372:392	2 biosimilar products (Flixabi®/Renflexis® and Remsima®/Inflectra®)	372:438	Therefore, critical quality attributes (structure and biological activity) of Remicade® and of 2 biosimilar products (Flixabi®/Renflexis® and Remsima®/Inflectra®) were determined.
28640663	3	87	theme	biosimilar	374:383	arg1	Remsima®/Inflectra®					419:437	Remsima®/Inflectra®	419:437	Remsima®/Inflectra®	419:437	Therefore, critical quality attributes (structure and biological activity) of Remicade® and of 2 biosimilar products (Flixabi®/Renflexis® and Remsima®/Inflectra®) were determined.
28640663	3	87	theme	biosimilar	374:383	arg1	Flixabi®/Renflexis®					395:413	Flixabi®/Renflexis®	395:413	Flixabi®/Renflexis®	395:413	Therefore, critical quality attributes (structure and biological activity) of Remicade® and of 2 biosimilar products (Flixabi®/Renflexis® and Remsima®/Inflectra®) were determined.
28640663	7	88	theme	cell	1243:1246	arg1	line					1248:1251	a CHO cell line	1237:1251	a CHO cell line	1237:1251	Whereas Remsima® and Remicade® are expressed in a Sp2/0 cell line, Flixabi® is expressed in a CHO cell line.
28640663	4	89	from	potency	547:553	arg1	assay					585:589	a luciferase reporter gene assay	558:589	a luciferase reporter gene assay	558:589	We assessed binding to tumor necrosis factor in a fluorescence competitive binding assay; potency in a luciferase reporter gene assay; percentages of galactosylated glycan, afucose plus high mannosylated glycans, and charged glycan; FcγRIIIa (CD16) binding (assessed by 3 methods); and antibody-dependent cell-mediated cytotoxicity (ADCC) in the NK92-CD16a cell line and in peripheral blood mononuclear cells (PBMC).
28640663	12	90	theme	afucosylated	1762:1773	arg1	glycans					1797:1803	afucosylated and high mannosylated glycans	1762:1803	afucosylated and high mannosylated glycans	1762:1803	Combined percentages of afucosylated and high mannosylated glycans were positively correlated with FcγRIIIa binding and ADCC in NK92-CD16 cells, while no correlation was observed in PBMC.
28640663	8	91	theme	expression	1351:1360	arg1	system					1362:1367	the expression system	1347:1367	the expression system	1347:1367	Despite this difference, galactosylated glycans from the 3 products were not correlated with the expression system.
28640663	12	92	theme	high	1779:1782	arg1	glycans					1797:1803	afucosylated and high mannosylated glycans	1762:1803	afucosylated and high mannosylated glycans	1762:1803	Combined percentages of afucosylated and high mannosylated glycans were positively correlated with FcγRIIIa binding and ADCC in NK92-CD16 cells, while no correlation was observed in PBMC.
28640663	10	93	from	percentage	1499:1508	arg1	cells					1532:1536	NK92-CD16a cells	1521:1536	NK92-CD16a cells	1521:1536	The percentage of ADCC in NK92-CD16a cells was lower with Remsima® and higher with Flixabi® compared with Remicade®, but was similar for all 3 products in PBMC.
28640663	12	94	theme	Combined	1738:1745	arg1	percentages					1747:1757	Combined percentages	1738:1757	Combined percentages of afucosylated and high mannosylated glycans	1738:1803	Combined percentages of afucosylated and high mannosylated glycans were positively correlated with FcγRIIIa binding and ADCC in NK92-CD16 cells, while no correlation was observed in PBMC.
28640663	6	95	theme	lower	1085:1089	arg1	percentage					1091:1100	a lower percentage	1083:1100	a lower percentage of afucose plus high mannosylated glycans	1083:1142	Compared with Remicade®, Flixabi® had a lower percentage of charged glycan, and Remsima® had a higher percentage of galactosylated glycan and a lower percentage of afucose plus high mannosylated glycans.
28640663	10	96	from	ADCC	1513:1516	arg1	cells					1532:1536	NK92-CD16a cells	1521:1536	NK92-CD16a cells	1521:1536	The percentage of ADCC in NK92-CD16a cells was lower with Remsima® and higher with Flixabi® compared with Remicade®, but was similar for all 3 products in PBMC.
29120786	0	0	theme	wheat	68:72	arg1	composition					32:42	the dietary fibre composition	14:42	the dietary fibre composition of old and modern durum wheat (Triticum turgidum spp	14:95	Comparison of the dietary fibre composition of old and modern durum wheat (Triticum turgidum spp.
29120786	4	1	from	β-glucan	583:590	arg1	semolina					595:602	semolina	595:602	semolina	595:602	No differences in total arabinoxylan content were observed but the modern varieties had higher proportions of soluble arabinoxylan in wholemeals and of β-glucan in semolina.
29120786	5	2	theme	durum	742:746	arg1	wheat					748:752	durum wheat	742:752	durum wheat	742:752	The study therefore provides no evidence that intensive breeding has had negative effects on the contents of dietary fibre components in durum wheat.
29120786	4	3	theme	total	449:453	arg1	content					468:474	total arabinoxylan content	449:474	total arabinoxylan content	449:474	No differences in total arabinoxylan content were observed but the modern varieties had higher proportions of soluble arabinoxylan in wholemeals and of β-glucan in semolina.
29120786	5	4	theme	fibre	722:726	arg1	components					728:737	dietary fibre components	714:737	dietary fibre components	714:737	The study therefore provides no evidence that intensive breeding has had negative effects on the contents of dietary fibre components in durum wheat.
29120786	3	5	theme	major	300:304	arg1	β-glucan					349:356	β-glucan	349:356	β-glucan	349:356	We therefore compared the contents and compositions of the major dietary fibre components, arabinoxylan and β-glucan, in semolina and wholemeal flour of old and modern Italian durum wheats.
29120786	3	5	theme	major	300:304	arg1	arabinoxylan					332:343	arabinoxylan	332:343	arabinoxylan	332:343	We therefore compared the contents and compositions of the major dietary fibre components, arabinoxylan and β-glucan, in semolina and wholemeal flour of old and modern Italian durum wheats.
29120786	3	5	theme	major	300:304	arg1	components					320:329	the major dietary fibre components	296:329	the major dietary fibre components	296:329	We therefore compared the contents and compositions of the major dietary fibre components, arabinoxylan and β-glucan, in semolina and wholemeal flour of old and modern Italian durum wheats.
29120786	6	6	theme	modern	922:927	arg1	genotypes					929:937	the modern genotypes	918:937	the modern genotypes	918:937	However, comparison of material grown over two years indicated that the content and composition of arabinoxylan and β-glucan were more stable in the older than in the modern genotypes.
29120786	7	7	theme	human	1091:1095	arg1	health					1097:1102	human health	1091:1102	human health	1091:1102	The identification of modern cultivars with high viscosity associated with a high content of β-glucan suggests that they are good sources of fibre for human health.
29120786	4	8	theme	arabinoxylan	549:560	arg1	proportions					526:536	higher proportions	519:536	higher proportions of soluble arabinoxylan in wholemeals and of β-glucan in semolina	519:602	No differences in total arabinoxylan content were observed but the modern varieties had higher proportions of soluble arabinoxylan in wholemeals and of β-glucan in semolina.
29120786	3	9	dep	contents	267:274	arg1	the					263:265	the	263:265	the	263:265	We therefore compared the contents and compositions of the major dietary fibre components, arabinoxylan and β-glucan, in semolina and wholemeal flour of old and modern Italian durum wheats.
29120786	4	10	from	wholemeals	565:574	arg1	proportions					526:536	higher proportions	519:536	higher proportions of soluble arabinoxylan in wholemeals and of β-glucan in semolina	519:602	No differences in total arabinoxylan content were observed but the modern varieties had higher proportions of soluble arabinoxylan in wholemeals and of β-glucan in semolina.
29120786	6	11	theme	β-glucan	871:878	arg1	content					827:833	content	827:833	content	827:833	However, comparison of material grown over two years indicated that the content and composition of arabinoxylan and β-glucan were more stable in the older than in the modern genotypes.
29120786	6	11	theme	β-glucan	871:878	arg1	composition					839:849	composition	839:849	composition	839:849	However, comparison of material grown over two years indicated that the content and composition of arabinoxylan and β-glucan were more stable in the older than in the modern genotypes.
29120786	6	12	theme	material	778:785	arg1	comparison					764:773	comparison	764:773	comparison of material grown over two years	764:806	However, comparison of material grown over two years indicated that the content and composition of arabinoxylan and β-glucan were more stable in the older than in the modern genotypes.
29120786	4	13	contain	had	515:517	arg1	varieties					505:513	the modern varieties	494:513	the modern varieties	494:513	No differences in total arabinoxylan content were observed but the modern varieties had higher proportions of soluble arabinoxylan in wholemeals and of β-glucan in semolina.
29120786	4	13	contain	had	515:517	arg2	proportions					526:536	higher proportions	519:536	higher proportions of soluble arabinoxylan in wholemeals and of β-glucan in semolina	519:602	No differences in total arabinoxylan content were observed but the modern varieties had higher proportions of soluble arabinoxylan in wholemeals and of β-glucan in semolina.
29120786	3	14	theme	components	320:329	arg1	compositions					280:291	compositions	280:291	compositions	280:291	We therefore compared the contents and compositions of the major dietary fibre components, arabinoxylan and β-glucan, in semolina and wholemeal flour of old and modern Italian durum wheats.
29120786	3	14	theme	components	320:329	arg1	contents					267:274	contents	267:274	contents	267:274	We therefore compared the contents and compositions of the major dietary fibre components, arabinoxylan and β-glucan, in semolina and wholemeal flour of old and modern Italian durum wheats.
29120786	4	15	from	proportions	526:536	arg1	wholemeals					565:574	wholemeals	565:574	wholemeals	565:574	No differences in total arabinoxylan content were observed but the modern varieties had higher proportions of soluble arabinoxylan in wholemeals and of β-glucan in semolina.
29120786	4	15	from	proportions	526:536	arg1	semolina					595:602	semolina	595:602	semolina	595:602	No differences in total arabinoxylan content were observed but the modern varieties had higher proportions of soluble arabinoxylan in wholemeals and of β-glucan in semolina.
29120786	0	16	dep	Triticum	75:82	arg1	spp					93:95	Triticum turgidum spp	75:95	Triticum turgidum spp	75:95	Comparison of the dietary fibre composition of old and modern durum wheat (Triticum turgidum spp.
29120786	2	17	from	contents	183:190	arg1	wheats					233:238	modern wheats	226:238	modern wheats	226:238	It has been suggested that intensive breeding has led to decreased contents of health-promoting components in modern wheats.
29120786	3	18	theme	modern	402:407	arg1	wheats					423:428	old and modern Italian durum wheats	394:428	old and modern Italian durum wheats	394:428	We therefore compared the contents and compositions of the major dietary fibre components, arabinoxylan and β-glucan, in semolina and wholemeal flour of old and modern Italian durum wheats.
29120786	4	19	from	arabinoxylan	549:560	arg1	wholemeals					565:574	wholemeals	565:574	wholemeals	565:574	No differences in total arabinoxylan content were observed but the modern varieties had higher proportions of soluble arabinoxylan in wholemeals and of β-glucan in semolina.
29120786	4	20	theme	arabinoxylan	455:466	arg1	content					468:474	total arabinoxylan content	449:474	total arabinoxylan content	449:474	No differences in total arabinoxylan content were observed but the modern varieties had higher proportions of soluble arabinoxylan in wholemeals and of β-glucan in semolina.
29120786	7	21	theme	good	1065:1068	arg1	they					1056:1059	they	1056:1059	they	1056:1059	The identification of modern cultivars with high viscosity associated with a high content of β-glucan suggests that they are good sources of fibre for human health.
29120786	7	21	theme	good	1065:1068	arg1	sources					1070:1076	good sources	1065:1076	good sources of fibre for human health	1065:1102	The identification of modern cultivars with high viscosity associated with a high content of β-glucan suggests that they are good sources of fibre for human health.
29120786	2	22	from	components	212:221	arg1	wheats					233:238	modern wheats	226:238	modern wheats	226:238	It has been suggested that intensive breeding has led to decreased contents of health-promoting components in modern wheats.
29120786	3	23	theme	Italian	409:415	arg1	wheats					423:428	old and modern Italian durum wheats	394:428	old and modern Italian durum wheats	394:428	We therefore compared the contents and compositions of the major dietary fibre components, arabinoxylan and β-glucan, in semolina and wholemeal flour of old and modern Italian durum wheats.
29120786	0	24	theme	durum	62:66	arg1	wheat					68:72	durum wheat	62:72	old and modern durum wheat (Triticum turgidum spp	47:95	Comparison of the dietary fibre composition of old and modern durum wheat (Triticum turgidum spp.
29120786	3	25	theme	dietary	306:312	arg1	β-glucan					349:356	β-glucan	349:356	β-glucan	349:356	We therefore compared the contents and compositions of the major dietary fibre components, arabinoxylan and β-glucan, in semolina and wholemeal flour of old and modern Italian durum wheats.
29120786	3	25	theme	dietary	306:312	arg1	arabinoxylan					332:343	arabinoxylan	332:343	arabinoxylan	332:343	We therefore compared the contents and compositions of the major dietary fibre components, arabinoxylan and β-glucan, in semolina and wholemeal flour of old and modern Italian durum wheats.
29120786	3	25	theme	dietary	306:312	arg1	components					320:329	the major dietary fibre components	296:329	the major dietary fibre components	296:329	We therefore compared the contents and compositions of the major dietary fibre components, arabinoxylan and β-glucan, in semolina and wholemeal flour of old and modern Italian durum wheats.
29120786	0	26	dep	wheat	68:72	arg1	Triticum					75:82	Triticum	75:82	Triticum	75:82	Comparison of the dietary fibre composition of old and modern durum wheat (Triticum turgidum spp.
29120786	7	27	theme	high	1017:1020	arg1	content					1022:1028	a high content	1015:1028	a high content of β-glucan	1015:1040	The identification of modern cultivars with high viscosity associated with a high content of β-glucan suggests that they are good sources of fibre for human health.
29120786	3	28	theme	old	394:396	arg1	wheats					423:428	old and modern Italian durum wheats	394:428	old and modern Italian durum wheats	394:428	We therefore compared the contents and compositions of the major dietary fibre components, arabinoxylan and β-glucan, in semolina and wholemeal flour of old and modern Italian durum wheats.
29120786	4	29	from	differences	434:444	arg1	content					468:474	total arabinoxylan content	449:474	total arabinoxylan content	449:474	No differences in total arabinoxylan content were observed but the modern varieties had higher proportions of soluble arabinoxylan in wholemeals and of β-glucan in semolina.
29120786	0	30	theme	dietary	18:24	arg1	composition					32:42	the dietary fibre composition	14:42	the dietary fibre composition of old and modern durum wheat (Triticum turgidum spp	14:95	Comparison of the dietary fibre composition of old and modern durum wheat (Triticum turgidum spp.
29120786	4	31	theme	higher	519:524	arg1	proportions					526:536	higher proportions	519:536	higher proportions of soluble arabinoxylan in wholemeals and of β-glucan in semolina	519:602	No differences in total arabinoxylan content were observed but the modern varieties had higher proportions of soluble arabinoxylan in wholemeals and of β-glucan in semolina.
29120786	5	32	theme	negative	678:685	arg1	effects					687:693	negative effects	678:693	negative effects	678:693	The study therefore provides no evidence that intensive breeding has had negative effects on the contents of dietary fibre components in durum wheat.
29120786	5	33	theme	components	728:737	arg1	contents					702:709	the contents	698:709	the contents of dietary fibre components in durum wheat	698:752	The study therefore provides no evidence that intensive breeding has had negative effects on the contents of dietary fibre components in durum wheat.
29120786	4	34	theme	soluble	541:547	arg1	arabinoxylan					549:560	soluble arabinoxylan	541:560	soluble arabinoxylan in wholemeals	541:574	No differences in total arabinoxylan content were observed but the modern varieties had higher proportions of soluble arabinoxylan in wholemeals and of β-glucan in semolina.
29120786	7	35	theme	cultivars	969:977	arg1	identification					944:957	The identification	940:957	The identification of modern cultivars with high viscosity associated with a high content of β-glucan	940:1040	The identification of modern cultivars with high viscosity associated with a high content of β-glucan suggests that they are good sources of fibre for human health.
29120786	0	36	theme	composition	32:42	arg1	Comparison					0:9	Comparison	0:9	Comparison of the dietary fibre composition of old and modern durum wheat (Triticum turgidum spp.	0:96	Comparison of the dietary fibre composition of old and modern durum wheat (Triticum turgidum spp.
29120786	6	37	dep	content	827:833	arg1	the					823:825	the	823:825	the	823:825	However, comparison of material grown over two years indicated that the content and composition of arabinoxylan and β-glucan were more stable in the older than in the modern genotypes.
29120786	2	38	theme	intensive	143:151	arg1	breeding					153:160	intensive breeding	143:160	intensive breeding	143:160	It has been suggested that intensive breeding has led to decreased contents of health-promoting components in modern wheats.
29120786	2	39	theme	components	212:221	arg1	contents					183:190	decreased contents	173:190	decreased contents of health-promoting components in modern wheats	173:238	It has been suggested that intensive breeding has led to decreased contents of health-promoting components in modern wheats.
29120786	2	40	from	wheats	233:238	arg1	contents					183:190	decreased contents	173:190	decreased contents of health-promoting components in modern wheats	173:238	It has been suggested that intensive breeding has led to decreased contents of health-promoting components in modern wheats.
29120786	3	41	theme	durum	417:421	arg1	wheats					423:428	old and modern Italian durum wheats	394:428	old and modern Italian durum wheats	394:428	We therefore compared the contents and compositions of the major dietary fibre components, arabinoxylan and β-glucan, in semolina and wholemeal flour of old and modern Italian durum wheats.
29120786	0	42	theme	fibre	26:30	arg1	composition					32:42	the dietary fibre composition	14:42	the dietary fibre composition of old and modern durum wheat (Triticum turgidum spp	14:95	Comparison of the dietary fibre composition of old and modern durum wheat (Triticum turgidum spp.
29120786	4	43	theme	modern	498:503	arg1	varieties					505:513	the modern varieties	494:513	the modern varieties	494:513	No differences in total arabinoxylan content were observed but the modern varieties had higher proportions of soluble arabinoxylan in wholemeals and of β-glucan in semolina.
29120786	4	44	from	semolina	595:602	arg1	proportions					526:536	higher proportions	519:536	higher proportions of soluble arabinoxylan in wholemeals and of β-glucan in semolina	519:602	No differences in total arabinoxylan content were observed but the modern varieties had higher proportions of soluble arabinoxylan in wholemeals and of β-glucan in semolina.
29120786	2	45	theme	health-promoting	195:210	arg1	components					212:221	health-promoting components	195:221	health-promoting components in modern wheats	195:238	It has been suggested that intensive breeding has led to decreased contents of health-promoting components in modern wheats.
29120786	7	46	theme	high	984:987	arg1	viscosity					989:997	high viscosity	984:997	high viscosity associated with a high content of β-glucan	984:1040	The identification of modern cultivars with high viscosity associated with a high content of β-glucan suggests that they are good sources of fibre for human health.
29120786	3	47	theme	wheats	423:428	arg1	semolina					362:369	semolina	362:369	semolina	362:369	We therefore compared the contents and compositions of the major dietary fibre components, arabinoxylan and β-glucan, in semolina and wholemeal flour of old and modern Italian durum wheats.
29120786	3	47	theme	wheats	423:428	arg1	flour					385:389	wholemeal flour	375:389	wholemeal flour	375:389	We therefore compared the contents and compositions of the major dietary fibre components, arabinoxylan and β-glucan, in semolina and wholemeal flour of old and modern Italian durum wheats.
29120786	0	48	theme	old	47:49	arg1	wheat					68:72	durum wheat	62:72	old and modern durum wheat (Triticum turgidum spp	47:95	Comparison of the dietary fibre composition of old and modern durum wheat (Triticum turgidum spp.
29120786	5	49	contain	had	674:676	arg2	effects					687:693	negative effects	678:693	negative effects	678:693	The study therefore provides no evidence that intensive breeding has had negative effects on the contents of dietary fibre components in durum wheat.
29120786	5	49	contain	had	674:676	arg1	breeding					661:668	intensive breeding	651:668	intensive breeding	651:668	The study therefore provides no evidence that intensive breeding has had negative effects on the contents of dietary fibre components in durum wheat.
29120786	5	50	theme	intensive	651:659	arg1	breeding					661:668	intensive breeding	651:668	intensive breeding	651:668	The study therefore provides no evidence that intensive breeding has had negative effects on the contents of dietary fibre components in durum wheat.
29120786	6	51	theme	arabinoxylan	854:865	arg1	content					827:833	content	827:833	content	827:833	However, comparison of material grown over two years indicated that the content and composition of arabinoxylan and β-glucan were more stable in the older than in the modern genotypes.
29120786	6	51	theme	arabinoxylan	854:865	arg1	composition					839:849	composition	839:849	composition	839:849	However, comparison of material grown over two years indicated that the content and composition of arabinoxylan and β-glucan were more stable in the older than in the modern genotypes.
29120786	3	52	theme	wholemeal	375:383	arg1	flour					385:389	wholemeal flour	375:389	wholemeal flour	375:389	We therefore compared the contents and compositions of the major dietary fibre components, arabinoxylan and β-glucan, in semolina and wholemeal flour of old and modern Italian durum wheats.
29120786	7	53	theme	β-glucan	1033:1040	arg1	content					1022:1028	a high content	1015:1028	a high content of β-glucan	1015:1040	The identification of modern cultivars with high viscosity associated with a high content of β-glucan suggests that they are good sources of fibre for human health.
29120786	2	54	theme	decreased	173:181	arg1	contents					183:190	decreased contents	173:190	decreased contents of health-promoting components in modern wheats	173:238	It has been suggested that intensive breeding has led to decreased contents of health-promoting components in modern wheats.
29120786	6	55	from	stable	890:895	arg1	older					904:908	older	904:908	older	904:908	However, comparison of material grown over two years indicated that the content and composition of arabinoxylan and β-glucan were more stable in the older than in the modern genotypes.
29120786	6	56	from	older	904:908	arg1	stable					890:895	stable	890:895	stable	890:895	However, comparison of material grown over two years indicated that the content and composition of arabinoxylan and β-glucan were more stable in the older than in the modern genotypes.
29120786	5	57	from	contents	702:709	arg1	wheat					748:752	durum wheat	742:752	durum wheat	742:752	The study therefore provides no evidence that intensive breeding has had negative effects on the contents of dietary fibre components in durum wheat.
29120786	3	58	from	contents	267:274	arg1	semolina					362:369	semolina	362:369	semolina	362:369	We therefore compared the contents and compositions of the major dietary fibre components, arabinoxylan and β-glucan, in semolina and wholemeal flour of old and modern Italian durum wheats.
29120786	3	58	from	contents	267:274	arg1	flour					385:389	wholemeal flour	375:389	wholemeal flour	375:389	We therefore compared the contents and compositions of the major dietary fibre components, arabinoxylan and β-glucan, in semolina and wholemeal flour of old and modern Italian durum wheats.
29120786	7	59	theme	fibre	1081:1085	arg1	they					1056:1059	they	1056:1059	they	1056:1059	The identification of modern cultivars with high viscosity associated with a high content of β-glucan suggests that they are good sources of fibre for human health.
29120786	7	59	theme	fibre	1081:1085	arg1	sources					1070:1076	good sources	1065:1076	good sources of fibre for human health	1065:1102	The identification of modern cultivars with high viscosity associated with a high content of β-glucan suggests that they are good sources of fibre for human health.
29120786	7	60	with	identification	944:957	arg1	viscosity					989:997	high viscosity	984:997	high viscosity associated with a high content of β-glucan	984:1040	The identification of modern cultivars with high viscosity associated with a high content of β-glucan suggests that they are good sources of fibre for human health.
29120786	0	61	theme	modern	55:60	arg1	wheat					68:72	durum wheat	62:72	old and modern durum wheat (Triticum turgidum spp	47:95	Comparison of the dietary fibre composition of old and modern durum wheat (Triticum turgidum spp.
29120786	2	62	theme	modern	226:231	arg1	wheats					233:238	modern wheats	226:238	modern wheats	226:238	It has been suggested that intensive breeding has led to decreased contents of health-promoting components in modern wheats.
29120786	7	63	theme	modern	962:967	arg1	cultivars					969:977	modern cultivars	962:977	modern cultivars	962:977	The identification of modern cultivars with high viscosity associated with a high content of β-glucan suggests that they are good sources of fibre for human health.
29120786	3	64	from	compositions	280:291	arg1	semolina					362:369	semolina	362:369	semolina	362:369	We therefore compared the contents and compositions of the major dietary fibre components, arabinoxylan and β-glucan, in semolina and wholemeal flour of old and modern Italian durum wheats.
29120786	3	64	from	compositions	280:291	arg1	flour					385:389	wholemeal flour	375:389	wholemeal flour	375:389	We therefore compared the contents and compositions of the major dietary fibre components, arabinoxylan and β-glucan, in semolina and wholemeal flour of old and modern Italian durum wheats.
29120786	3	65	theme	fibre	314:318	arg1	β-glucan					349:356	β-glucan	349:356	β-glucan	349:356	We therefore compared the contents and compositions of the major dietary fibre components, arabinoxylan and β-glucan, in semolina and wholemeal flour of old and modern Italian durum wheats.
29120786	3	65	theme	fibre	314:318	arg1	arabinoxylan					332:343	arabinoxylan	332:343	arabinoxylan	332:343	We therefore compared the contents and compositions of the major dietary fibre components, arabinoxylan and β-glucan, in semolina and wholemeal flour of old and modern Italian durum wheats.
29120786	3	65	theme	fibre	314:318	arg1	components					320:329	the major dietary fibre components	296:329	the major dietary fibre components	296:329	We therefore compared the contents and compositions of the major dietary fibre components, arabinoxylan and β-glucan, in semolina and wholemeal flour of old and modern Italian durum wheats.
29120786	4	66	theme	β-glucan	583:590	arg1	proportions					526:536	higher proportions	519:536	higher proportions of soluble arabinoxylan in wholemeals and of β-glucan in semolina	519:602	No differences in total arabinoxylan content were observed but the modern varieties had higher proportions of soluble arabinoxylan in wholemeals and of β-glucan in semolina.
29120786	5	67	theme	dietary	714:720	arg1	components					728:737	dietary fibre components	714:737	dietary fibre components	714:737	The study therefore provides no evidence that intensive breeding has had negative effects on the contents of dietary fibre components in durum wheat.
28416396	9	0	theme	MG	1328:1329	arg1	proliferation					1339:1351	the MG 63 cell proliferation	1324:1351	the MG 63 cell proliferation	1324:1351	The fabricated scaffolds were also found to encourage the MG 63 cell proliferation.
28416396	10	1	theme	practical	1479:1487	arg1	application					1489:1499	wider and more practical application	1464:1499	wider and more practical application	1464:1499	Hence, from the above studies it can be concluded that the CS/HA/MMT composite 3D macrospheric scaffolds have wider and more practical application in bone tissue regeneration applications.
28416396	7	2	theme	swelling	1023:1030	arg1	ability					1032:1038	the swelling ability	1019:1038	the swelling ability of the scaffolds which played an important role in maintaining the mechanical strength of the 3D scaffolds	1019:1145	The sintering of the macrospheres controlled the swelling ability of the scaffolds which played an important role in maintaining the mechanical strength of the 3D scaffolds.
28416396	2	3	dep	montmorillonite	307:321	arg1	combinations					353:364	combinations	353:364	combinations	353:364	Chitosan (CS) based composites were developed by varying montmorillonite (MMT) and hydroxyapatite (HA) combinations to fabricate macrospheric three dimensional (3D) scaffolds by direct agglomeration of the sintered macrospheres.
28416396	6	4	theme	hemocompatibility	932:948	arg1	adsorption					962:971	improved hemocompatibility and protein adsorption	923:971	improved hemocompatibility and protein adsorption	923:971	It was found that in comparison to pure CS, the CS/HA/MMT composites exhibited improved hemocompatibility and protein adsorption.
28416396	10	5	theme	bone	1504:1507	arg1	regeneration					1516:1527	bone tissue regeneration	1504:1527	bone tissue regeneration applications	1504:1540	Hence, from the above studies it can be concluded that the CS/HA/MMT composite 3D macrospheric scaffolds have wider and more practical application in bone tissue regeneration applications.
28416396	7	6	theme	macrospheres	995:1006	arg1	sintering					978:986	The sintering	974:986	The sintering of the macrospheres	974:1006	The sintering of the macrospheres controlled the swelling ability of the scaffolds which played an important role in maintaining the mechanical strength of the 3D scaffolds.
28416396	1	7	theme	controllable	170:181	arg1	architecture					208:219	controllable and predictable internal architecture	170:219	controllable and predictable internal architecture for bone tissue engineering	170:247	The present work deals with the fabrication of chitosan composite scaffolds with controllable and predictable internal architecture for bone tissue engineering.
28416396	6	8	theme	improved	923:930	arg1	adsorption					962:971	improved hemocompatibility and protein adsorption	923:971	improved hemocompatibility and protein adsorption	923:971	It was found that in comparison to pure CS, the CS/HA/MMT composites exhibited improved hemocompatibility and protein adsorption.
28416396	3	9	theme	CS/MMT/HA	516:524	arg1	scaffolds					529:537	The fabricated CS, CS/MMT, CS/HA and CS/MMT/HA 3D scaffolds	479:537	The fabricated CS, CS/MMT, CS/HA and CS/MMT/HA 3D scaffolds	479:537	The fabricated CS, CS/MMT, CS/HA and CS/MMT/HA 3D scaffolds were characterized for their physicochemical, biological and mechanical properties.
28416396	9	10	theme	cell	1334:1337	arg1	proliferation					1339:1351	the MG 63 cell proliferation	1324:1351	the MG 63 cell proliferation	1324:1351	The fabricated scaffolds were also found to encourage the MG 63 cell proliferation.
28416396	1	11	with	scaffolds	155:163	arg1	architecture					208:219	controllable and predictable internal architecture	170:219	controllable and predictable internal architecture for bone tissue engineering	170:247	The present work deals with the fabrication of chitosan composite scaffolds with controllable and predictable internal architecture for bone tissue engineering.
28416396	3	12	theme	CS/HA	506:510	arg1	scaffolds					529:537	The fabricated CS, CS/MMT, CS/HA and CS/MMT/HA 3D scaffolds	479:537	The fabricated CS, CS/MMT, CS/HA and CS/MMT/HA 3D scaffolds	479:537	The fabricated CS, CS/MMT, CS/HA and CS/MMT/HA 3D scaffolds were characterized for their physicochemical, biological and mechanical properties.
28416396	10	13	contain	have	1459:1462	arg1	scaffolds					1449:1457	the CS/HA/MMT composite 3D macrospheric scaffolds	1409:1457	the CS/HA/MMT composite 3D macrospheric scaffolds	1409:1457	Hence, from the above studies it can be concluded that the CS/HA/MMT composite 3D macrospheric scaffolds have wider and more practical application in bone tissue regeneration applications.
28416396	10	13	contain	have	1459:1462	arg2	application					1489:1499	wider and more practical application	1464:1499	wider and more practical application	1464:1499	Hence, from the above studies it can be concluded that the CS/HA/MMT composite 3D macrospheric scaffolds have wider and more practical application in bone tissue regeneration applications.
28416396	4	14	theme	molecular	714:722	arg1	interaction					724:734	the molecular interaction	710:734	the molecular interaction between them	710:747	The XRD and ATR-FTIR studies confirmed the presence of the individual constituents and the molecular interaction between them, respectively.
28416396	7	15	theme	3D	1134:1135	arg1	scaffolds					1137:1145	the 3D scaffolds	1130:1145	the 3D scaffolds	1130:1145	The sintering of the macrospheres controlled the swelling ability of the scaffolds which played an important role in maintaining the mechanical strength of the 3D scaffolds.
28416396	3	16	theme	fabricated	483:492	arg1	scaffolds					529:537	The fabricated CS, CS/MMT, CS/HA and CS/MMT/HA 3D scaffolds	479:537	The fabricated CS, CS/MMT, CS/HA and CS/MMT/HA 3D scaffolds	479:537	The fabricated CS, CS/MMT, CS/HA and CS/MMT/HA 3D scaffolds were characterized for their physicochemical, biological and mechanical properties.
28416396	2	17	theme	dimensional	398:408	arg1	scaffolds					415:423	macrospheric three dimensional (3D) scaffolds	379:423	macrospheric three dimensional (3D) scaffolds	379:423	Chitosan (CS) based composites were developed by varying montmorillonite (MMT) and hydroxyapatite (HA) combinations to fabricate macrospheric three dimensional (3D) scaffolds by direct agglomeration of the sintered macrospheres.
28416396	2	18	theme	3D	411:412	arg1	scaffolds					415:423	macrospheric three dimensional (3D) scaffolds	379:423	macrospheric three dimensional (3D) scaffolds	379:423	Chitosan (CS) based composites were developed by varying montmorillonite (MMT) and hydroxyapatite (HA) combinations to fabricate macrospheric three dimensional (3D) scaffolds by direct agglomeration of the sintered macrospheres.
28416396	5	19	theme	degradation	826:836	arg1	rate					838:841	degradation rate	826:841	degradation rate	826:841	The reinforcement with HA and MMT showed reduced swelling and degradation rate.
28416396	6	20	theme	CS/HA/MMT	892:900	arg1	composites					902:911	the CS/HA/MMT composites	888:911	the CS/HA/MMT composites	888:911	It was found that in comparison to pure CS, the CS/HA/MMT composites exhibited improved hemocompatibility and protein adsorption.
28416396	7	21	theme	scaffolds	1137:1145	arg1	strength					1118:1125	the mechanical strength	1103:1125	the mechanical strength of the 3D scaffolds	1103:1145	The sintering of the macrospheres controlled the swelling ability of the scaffolds which played an important role in maintaining the mechanical strength of the 3D scaffolds.
28416396	10	22	theme	regeneration	1516:1527	arg1	applications					1529:1540	bone tissue regeneration applications	1504:1540	bone tissue regeneration applications	1504:1540	Hence, from the above studies it can be concluded that the CS/HA/MMT composite 3D macrospheric scaffolds have wider and more practical application in bone tissue regeneration applications.
28416396	1	23	theme	predictable	187:197	arg1	architecture					208:219	controllable and predictable internal architecture	170:219	controllable and predictable internal architecture for bone tissue engineering	170:247	The present work deals with the fabrication of chitosan composite scaffolds with controllable and predictable internal architecture for bone tissue engineering.
28416396	2	24	theme	sintered	456:463	arg1	macrospheres					465:476	the sintered macrospheres	452:476	the sintered macrospheres	452:476	Chitosan (CS) based composites were developed by varying montmorillonite (MMT) and hydroxyapatite (HA) combinations to fabricate macrospheric three dimensional (3D) scaffolds by direct agglomeration of the sintered macrospheres.
28416396	3	25	theme	3D	526:527	arg1	scaffolds					529:537	The fabricated CS, CS/MMT, CS/HA and CS/MMT/HA 3D scaffolds	479:537	The fabricated CS, CS/MMT, CS/HA and CS/MMT/HA 3D scaffolds	479:537	The fabricated CS, CS/MMT, CS/HA and CS/MMT/HA 3D scaffolds were characterized for their physicochemical, biological and mechanical properties.
28416396	10	26	theme	wider	1464:1468	arg1	application					1489:1499	wider and more practical application	1464:1499	wider and more practical application	1464:1499	Hence, from the above studies it can be concluded that the CS/HA/MMT composite 3D macrospheric scaffolds have wider and more practical application in bone tissue regeneration applications.
28416396	1	27	theme	present	93:99	arg1	work					101:104	The present work	89:104	The present work	89:104	The present work deals with the fabrication of chitosan composite scaffolds with controllable and predictable internal architecture for bone tissue engineering.
28416396	1	28	theme	internal	199:206	arg1	architecture					208:219	controllable and predictable internal architecture	170:219	controllable and predictable internal architecture for bone tissue engineering	170:247	The present work deals with the fabrication of chitosan composite scaffolds with controllable and predictable internal architecture for bone tissue engineering.
28416396	10	29	theme	macrospheric	1436:1447	arg1	scaffolds					1449:1457	the CS/HA/MMT composite 3D macrospheric scaffolds	1409:1457	the CS/HA/MMT composite 3D macrospheric scaffolds	1409:1457	Hence, from the above studies it can be concluded that the CS/HA/MMT composite 3D macrospheric scaffolds have wider and more practical application in bone tissue regeneration applications.
28416396	3	30	theme	biological	585:594	arg1	properties					611:620	their physicochemical, biological and mechanical properties	562:620	their physicochemical, biological and mechanical properties	562:620	The fabricated CS, CS/MMT, CS/HA and CS/MMT/HA 3D scaffolds were characterized for their physicochemical, biological and mechanical properties.
28416396	4	31	theme	individual	682:691	arg1	constituents					693:704	the individual constituents	678:704	the individual constituents	678:704	The XRD and ATR-FTIR studies confirmed the presence of the individual constituents and the molecular interaction between them, respectively.
28416396	0	32	theme	dimensional	25:35	arg1	scaffolds					50:58	three dimensional macrospheric scaffolds	19:58	three dimensional macrospheric scaffolds for bone tissue engineering	19:86	Chitosan composite three dimensional macrospheric scaffolds for bone tissue engineering.
28416396	7	33	theme	mechanical	1107:1116	arg1	strength					1118:1125	the mechanical strength	1103:1125	the mechanical strength of the 3D scaffolds	1103:1145	The sintering of the macrospheres controlled the swelling ability of the scaffolds which played an important role in maintaining the mechanical strength of the 3D scaffolds.
28416396	10	34	theme	3D	1433:1434	arg1	scaffolds					1449:1457	the CS/HA/MMT composite 3D macrospheric scaffolds	1409:1457	the CS/HA/MMT composite 3D macrospheric scaffolds	1409:1457	Hence, from the above studies it can be concluded that the CS/HA/MMT composite 3D macrospheric scaffolds have wider and more practical application in bone tissue regeneration applications.
28416396	8	35	theme	CS	1256:1257	arg1	scaffolds					1259:1267	pure CS scaffolds	1251:1267	pure CS scaffolds	1251:1267	The CS/HA/MMT composite scaffold showed 14 folds increase in the compressive strength when compared to pure CS scaffolds.
28416396	10	36	theme	composite	1423:1431	arg1	scaffolds					1449:1457	the CS/HA/MMT composite 3D macrospheric scaffolds	1409:1457	the CS/HA/MMT composite 3D macrospheric scaffolds	1409:1457	Hence, from the above studies it can be concluded that the CS/HA/MMT composite 3D macrospheric scaffolds have wider and more practical application in bone tissue regeneration applications.
28416396	2	37	theme	direct	428:433	arg1	agglomeration					435:447	direct agglomeration	428:447	direct agglomeration of the sintered macrospheres	428:476	Chitosan (CS) based composites were developed by varying montmorillonite (MMT) and hydroxyapatite (HA) combinations to fabricate macrospheric three dimensional (3D) scaffolds by direct agglomeration of the sintered macrospheres.
28416396	3	38	theme	physicochemical	568:582	arg1	properties					611:620	their physicochemical, biological and mechanical properties	562:620	their physicochemical, biological and mechanical properties	562:620	The fabricated CS, CS/MMT, CS/HA and CS/MMT/HA 3D scaffolds were characterized for their physicochemical, biological and mechanical properties.
28416396	1	39	theme	bone	225:228	arg1	engineering					237:247	bone tissue engineering	225:247	bone tissue engineering	225:247	The present work deals with the fabrication of chitosan composite scaffolds with controllable and predictable internal architecture for bone tissue engineering.
28416396	5	40	theme	reduced	805:811	arg1	swelling					813:820	reduced swelling	805:820	reduced swelling	805:820	The reinforcement with HA and MMT showed reduced swelling and degradation rate.
28416396	4	41	theme	constituents	693:704	arg1	presence					666:673	the presence	662:673	the presence of the individual constituents	662:704	The XRD and ATR-FTIR studies confirmed the presence of the individual constituents and the molecular interaction between them, respectively.
28416396	4	41	theme	constituents	693:704	arg1	interaction					724:734	the molecular interaction	710:734	the molecular interaction between them	710:747	The XRD and ATR-FTIR studies confirmed the presence of the individual constituents and the molecular interaction between them, respectively.
28416396	9	42	theme	fabricated	1274:1283	arg1	scaffolds					1285:1293	The fabricated scaffolds	1270:1293	The fabricated scaffolds	1270:1293	The fabricated scaffolds were also found to encourage the MG 63 cell proliferation.
28416396	5	43	with	reinforcement	768:780	arg1	MMT					794:796	MMT	794:796	MMT	794:796	The reinforcement with HA and MMT showed reduced swelling and degradation rate.
28416396	5	43	with	reinforcement	768:780	arg1	HA					787:788	HA	787:788	HA	787:788	The reinforcement with HA and MMT showed reduced swelling and degradation rate.
28416396	1	44	theme	tissue	230:235	arg1	engineering					237:247	bone tissue engineering	225:247	bone tissue engineering	225:247	The present work deals with the fabrication of chitosan composite scaffolds with controllable and predictable internal architecture for bone tissue engineering.
28416396	0	45	theme	macrospheric	37:48	arg1	scaffolds					50:58	three dimensional macrospheric scaffolds	19:58	three dimensional macrospheric scaffolds for bone tissue engineering	19:86	Chitosan composite three dimensional macrospheric scaffolds for bone tissue engineering.
28416396	8	46	dep	14	1188:1189	arg1	folds					1191:1195	folds	1191:1195	folds increase in the compressive strength	1191:1232	The CS/HA/MMT composite scaffold showed 14 folds increase in the compressive strength when compared to pure CS scaffolds.
28416396	8	47	theme	pure	1251:1254	arg1	scaffolds					1259:1267	pure CS scaffolds	1251:1267	pure CS scaffolds	1251:1267	The CS/HA/MMT composite scaffold showed 14 folds increase in the compressive strength when compared to pure CS scaffolds.
28416396	6	48	theme	pure	879:882	arg1	CS					884:885	pure CS	879:885	pure CS	879:885	It was found that in comparison to pure CS, the CS/HA/MMT composites exhibited improved hemocompatibility and protein adsorption.
28416396	0	49	theme	bone	64:67	arg1	engineering					76:86	bone tissue engineering	64:86	bone tissue engineering	64:86	Chitosan composite three dimensional macrospheric scaffolds for bone tissue engineering.
28416396	2	50	theme	Chitosan	250:257	arg1	composites					270:279	Chitosan (CS) based composites	250:279	Chitosan (CS) based composites	250:279	Chitosan (CS) based composites were developed by varying montmorillonite (MMT) and hydroxyapatite (HA) combinations to fabricate macrospheric three dimensional (3D) scaffolds by direct agglomeration of the sintered macrospheres.
28416396	10	51	theme	above	1370:1374	arg1	studies					1376:1382	the above studies	1366:1382	the above studies	1366:1382	Hence, from the above studies it can be concluded that the CS/HA/MMT composite 3D macrospheric scaffolds have wider and more practical application in bone tissue regeneration applications.
28416396	3	52	theme	CS/MMT	498:503	arg1	scaffolds					529:537	The fabricated CS, CS/MMT, CS/HA and CS/MMT/HA 3D scaffolds	479:537	The fabricated CS, CS/MMT, CS/HA and CS/MMT/HA 3D scaffolds	479:537	The fabricated CS, CS/MMT, CS/HA and CS/MMT/HA 3D scaffolds were characterized for their physicochemical, biological and mechanical properties.
28416396	2	53	theme	macrospheres	465:476	arg1	agglomeration					435:447	direct agglomeration	428:447	direct agglomeration of the sintered macrospheres	428:476	Chitosan (CS) based composites were developed by varying montmorillonite (MMT) and hydroxyapatite (HA) combinations to fabricate macrospheric three dimensional (3D) scaffolds by direct agglomeration of the sintered macrospheres.
28416396	10	54	theme	CS/HA/MMT	1413:1421	arg1	scaffolds					1449:1457	the CS/HA/MMT composite 3D macrospheric scaffolds	1409:1457	the CS/HA/MMT composite 3D macrospheric scaffolds	1409:1457	Hence, from the above studies it can be concluded that the CS/HA/MMT composite 3D macrospheric scaffolds have wider and more practical application in bone tissue regeneration applications.
28416396	3	55	theme	mechanical	600:609	arg1	properties					611:620	their physicochemical, biological and mechanical properties	562:620	their physicochemical, biological and mechanical properties	562:620	The fabricated CS, CS/MMT, CS/HA and CS/MMT/HA 3D scaffolds were characterized for their physicochemical, biological and mechanical properties.
28416396	8	56	theme	composite	1162:1170	arg1	scaffold					1172:1179	The CS/HA/MMT composite scaffold	1148:1179	The CS/HA/MMT composite scaffold	1148:1179	The CS/HA/MMT composite scaffold showed 14 folds increase in the compressive strength when compared to pure CS scaffolds.
28416396	1	57	theme	composite	145:153	arg1	scaffolds					155:163	chitosan composite scaffolds	136:163	chitosan composite scaffolds with controllable and predictable internal architecture for bone tissue engineering	136:247	The present work deals with the fabrication of chitosan composite scaffolds with controllable and predictable internal architecture for bone tissue engineering.
28416396	7	58	theme	scaffolds	1047:1055	arg1	ability					1032:1038	the swelling ability	1019:1038	the swelling ability of the scaffolds which played an important role in maintaining the mechanical strength of the 3D scaffolds	1019:1145	The sintering of the macrospheres controlled the swelling ability of the scaffolds which played an important role in maintaining the mechanical strength of the 3D scaffolds.
28416396	3	59	theme	CS	494:495	arg1	scaffolds					529:537	The fabricated CS, CS/MMT, CS/HA and CS/MMT/HA 3D scaffolds	479:537	The fabricated CS, CS/MMT, CS/HA and CS/MMT/HA 3D scaffolds	479:537	The fabricated CS, CS/MMT, CS/HA and CS/MMT/HA 3D scaffolds were characterized for their physicochemical, biological and mechanical properties.
28416396	4	60	theme	XRD	627:629	arg1	studies					644:650	The XRD and ATR-FTIR studies	623:650	The XRD and ATR-FTIR studies	623:650	The XRD and ATR-FTIR studies confirmed the presence of the individual constituents and the molecular interaction between them, respectively.
28416396	7	61	theme	important	1073:1081	arg1	role					1083:1086	an important role	1070:1086	an important role	1070:1086	The sintering of the macrospheres controlled the swelling ability of the scaffolds which played an important role in maintaining the mechanical strength of the 3D scaffolds.
28416396	10	62	theme	tissue	1509:1514	arg1	regeneration					1516:1527	bone tissue regeneration	1504:1527	bone tissue regeneration applications	1504:1540	Hence, from the above studies it can be concluded that the CS/HA/MMT composite 3D macrospheric scaffolds have wider and more practical application in bone tissue regeneration applications.
28416396	1	63	theme	chitosan	136:143	arg1	scaffolds					155:163	chitosan composite scaffolds	136:163	chitosan composite scaffolds with controllable and predictable internal architecture for bone tissue engineering	136:247	The present work deals with the fabrication of chitosan composite scaffolds with controllable and predictable internal architecture for bone tissue engineering.
28416396	8	64	from	increase	1197:1204	arg1	strength					1225:1232	the compressive strength	1209:1232	the compressive strength	1209:1232	The CS/HA/MMT composite scaffold showed 14 folds increase in the compressive strength when compared to pure CS scaffolds.
28416396	2	65	theme	based	264:268	arg1	composites					270:279	Chitosan (CS) based composites	250:279	Chitosan (CS) based composites	250:279	Chitosan (CS) based composites were developed by varying montmorillonite (MMT) and hydroxyapatite (HA) combinations to fabricate macrospheric three dimensional (3D) scaffolds by direct agglomeration of the sintered macrospheres.
28416396	8	66	theme	compressive	1213:1223	arg1	strength					1225:1232	the compressive strength	1209:1232	the compressive strength	1209:1232	The CS/HA/MMT composite scaffold showed 14 folds increase in the compressive strength when compared to pure CS scaffolds.
28416396	2	67	theme	macrospheric	379:390	arg1	scaffolds					415:423	macrospheric three dimensional (3D) scaffolds	379:423	macrospheric three dimensional (3D) scaffolds	379:423	Chitosan (CS) based composites were developed by varying montmorillonite (MMT) and hydroxyapatite (HA) combinations to fabricate macrospheric three dimensional (3D) scaffolds by direct agglomeration of the sintered macrospheres.
28416396	4	68	theme	ATR-FTIR	635:642	arg1	studies					644:650	The XRD and ATR-FTIR studies	623:650	The XRD and ATR-FTIR studies	623:650	The XRD and ATR-FTIR studies confirmed the presence of the individual constituents and the molecular interaction between them, respectively.
28416396	0	69	theme	tissue	69:74	arg1	engineering					76:86	bone tissue engineering	64:86	bone tissue engineering	64:86	Chitosan composite three dimensional macrospheric scaffolds for bone tissue engineering.
28416396	6	70	theme	protein	954:960	arg1	adsorption					962:971	improved hemocompatibility and protein adsorption	923:971	improved hemocompatibility and protein adsorption	923:971	It was found that in comparison to pure CS, the CS/HA/MMT composites exhibited improved hemocompatibility and protein adsorption.
28416396	1	71	theme	scaffolds	155:163	arg1	fabrication					121:131	the fabrication	117:131	the fabrication of chitosan composite scaffolds with controllable and predictable internal architecture for bone tissue engineering	117:247	The present work deals with the fabrication of chitosan composite scaffolds with controllable and predictable internal architecture for bone tissue engineering.
28416396	8	72	theme	CS/HA/MMT	1152:1160	arg1	scaffold					1172:1179	The CS/HA/MMT composite scaffold	1148:1179	The CS/HA/MMT composite scaffold	1148:1179	The CS/HA/MMT composite scaffold showed 14 folds increase in the compressive strength when compared to pure CS scaffolds.
24528727	4	0	theme	Hz	819:820	arg1	range					794:798	the frequency range	780:798	the frequency range of 0.01 Hz to 10(5)Hz	780:820	The relaxation process of collagen in the frequency range of 0.01 Hz to 10(5)Hz and thermoporometry results indicate that the interfacial hydration dynamics are dependent on the applied concentration of LBG.
24528727	3	1	from	influence	667:675	arg1	collagen					687:694	collagen	687:694	collagen	687:694	Water, as a probe liquid bound in collagen has been investigated using a combination of thermoporometry, ATR-FTIR, circular dichroic spectroscopy, dielectric spectroscopy and SEM to explore the influence of LBG on collagen with respect to static and dynamic behaviour.
24528727	5	2	theme	amino	1060:1064	arg1	acids					1066:1070	the charged amino acids	1048:1070	the charged amino acids of collagen	1048:1082	This investigation explicitly reflects the rearrangements of the structural water clusters around the charged amino acids of collagen.
24528727	1	3	theme	hydration	165:173	arg1	dynamics					175:182	the hydration dynamics	161:182	the hydration dynamics of collagen	161:194	The intricacy of the different parameters involved in the hydration dynamics of collagen influences its performance as biomaterials.
24528727	3	4	theme	LBG	680:682	arg1	influence					667:675	the influence	663:675	the influence of LBG on collagen	663:694	Water, as a probe liquid bound in collagen has been investigated using a combination of thermoporometry, ATR-FTIR, circular dichroic spectroscopy, dielectric spectroscopy and SEM to explore the influence of LBG on collagen with respect to static and dynamic behaviour.
24528727	4	5	theme	thermoporometry	826:840	arg1	results					842:848	The relaxation process of collagen in the frequency range of 0.01 Hz to 10(5)Hz and thermoporometry results	742:848	The relaxation process of collagen in the frequency range of 0.01 Hz to 10(5)Hz and thermoporometry results	742:848	The relaxation process of collagen in the frequency range of 0.01 Hz to 10(5)Hz and thermoporometry results indicate that the interfacial hydration dynamics are dependent on the applied concentration of LBG.
24528727	4	6	theme	Hz	808:809	arg1	Hz					819:820	0.01 Hz to 10(5)Hz	803:820	0.01 Hz to 10(5)Hz	803:820	The relaxation process of collagen in the frequency range of 0.01 Hz to 10(5)Hz and thermoporometry results indicate that the interfacial hydration dynamics are dependent on the applied concentration of LBG.
24528727	4	7	theme	relaxation	746:755	arg1	process					757:763	The relaxation process	742:763	The relaxation process of collagen in the frequency range of 0.01 Hz to 10(5)Hz	742:820	The relaxation process of collagen in the frequency range of 0.01 Hz to 10(5)Hz and thermoporometry results indicate that the interfacial hydration dynamics are dependent on the applied concentration of LBG.
24528727	6	8	theme	biomaterials	1182:1193	arg1	development					1152:1162	the development	1148:1162	the development of collagen based biomaterials	1148:1193	These results can be employed to redesign the approach towards the development of collagen based biomaterials.
24528727	3	9	dep	investigated	525:536	arg1	bound					498:502	bound	498:502	bound in collagen	498:514	Water, as a probe liquid bound in collagen has been investigated using a combination of thermoporometry, ATR-FTIR, circular dichroic spectroscopy, dielectric spectroscopy and SEM to explore the influence of LBG on collagen with respect to static and dynamic behaviour.
24528727	4	10	theme	applied	920:926	arg1	concentration					928:940	the applied concentration	916:940	the applied concentration of LBG	916:947	The relaxation process of collagen in the frequency range of 0.01 Hz to 10(5)Hz and thermoporometry results indicate that the interfacial hydration dynamics are dependent on the applied concentration of LBG.
24528727	3	11	theme	static	712:717	arg1	behaviour					731:739	static and dynamic behaviour	712:739	static and dynamic behaviour	712:739	Water, as a probe liquid bound in collagen has been investigated using a combination of thermoporometry, ATR-FTIR, circular dichroic spectroscopy, dielectric spectroscopy and SEM to explore the influence of LBG on collagen with respect to static and dynamic behaviour.
24528727	4	12	theme	frequency	784:792	arg1	range					794:798	the frequency range	780:798	the frequency range of 0.01 Hz to 10(5)Hz	780:820	The relaxation process of collagen in the frequency range of 0.01 Hz to 10(5)Hz and thermoporometry results indicate that the interfacial hydration dynamics are dependent on the applied concentration of LBG.
24528727	2	13	theme	collagen	284:291	arg1	motions					273:279	the molecular motions	259:279	the molecular motions of collagen originating from the solvents and locust bean gum (LBG), which reveal the changes in solvation dynamics of the biopolymers affecting the surface as well as interfacial properties	259:470	This work presents the molecular motions of collagen originating from the solvents and locust bean gum (LBG), which reveal the changes in solvation dynamics of the biopolymers affecting the surface as well as interfacial properties.
24528727	2	14	theme	solvation	378:386	arg1	dynamics					388:395	solvation dynamics	378:395	solvation dynamics of the biopolymers affecting the surface as well as interfacial properties	378:470	This work presents the molecular motions of collagen originating from the solvents and locust bean gum (LBG), which reveal the changes in solvation dynamics of the biopolymers affecting the surface as well as interfacial properties.
24528727	4	15	theme	LBG	945:947	arg1	concentration					928:940	the applied concentration	916:940	the applied concentration of LBG	916:947	The relaxation process of collagen in the frequency range of 0.01 Hz to 10(5)Hz and thermoporometry results indicate that the interfacial hydration dynamics are dependent on the applied concentration of LBG.
24528727	2	16	theme	bean	334:337	arg1	LBG					344:346	LBG	344:346	LBG	344:346	This work presents the molecular motions of collagen originating from the solvents and locust bean gum (LBG), which reveal the changes in solvation dynamics of the biopolymers affecting the surface as well as interfacial properties.
24528727	2	16	theme	bean	334:337	arg1	gum					339:341	locust bean gum	327:341	locust bean gum (LBG)	327:347	This work presents the molecular motions of collagen originating from the solvents and locust bean gum (LBG), which reveal the changes in solvation dynamics of the biopolymers affecting the surface as well as interfacial properties.
24528727	5	17	theme	collagen	1075:1082	arg1	acids					1066:1070	the charged amino acids	1048:1070	the charged amino acids of collagen	1048:1082	This investigation explicitly reflects the rearrangements of the structural water clusters around the charged amino acids of collagen.
24528727	1	18	theme	collagen	187:194	arg1	dynamics					175:182	the hydration dynamics	161:182	the hydration dynamics of collagen	161:194	The intricacy of the different parameters involved in the hydration dynamics of collagen influences its performance as biomaterials.
24528727	6	19	theme	based	1176:1180	arg1	biomaterials					1182:1193	collagen based biomaterials	1167:1193	collagen based biomaterials	1167:1193	These results can be employed to redesign the approach towards the development of collagen based biomaterials.
24528727	2	20	theme	locust	327:332	arg1	LBG					344:346	LBG	344:346	LBG	344:346	This work presents the molecular motions of collagen originating from the solvents and locust bean gum (LBG), which reveal the changes in solvation dynamics of the biopolymers affecting the surface as well as interfacial properties.
24528727	2	20	theme	locust	327:332	arg1	gum					339:341	locust bean gum	327:341	locust bean gum (LBG)	327:347	This work presents the molecular motions of collagen originating from the solvents and locust bean gum (LBG), which reveal the changes in solvation dynamics of the biopolymers affecting the surface as well as interfacial properties.
24528727	5	21	theme	charged	1052:1058	arg1	acids					1066:1070	the charged amino acids	1048:1070	the charged amino acids of collagen	1048:1082	This investigation explicitly reflects the rearrangements of the structural water clusters around the charged amino acids of collagen.
24528727	0	22	theme	dynamics	25:32	arg1	Elucidation					0:10	Elucidation	0:10	Elucidation of hydration dynamics of locust bean gum-collagen composites by impedance and thermoporometry.	0:105	Elucidation of hydration dynamics of locust bean gum-collagen composites by impedance and thermoporometry.
24528727	3	23	theme	circular	588:595	arg1	spectroscopy					606:617	ATR-FTIR, circular dichroic spectroscopy	578:617	spectroscopy	606:617	Water, as a probe liquid bound in collagen has been investigated using a combination of thermoporometry, ATR-FTIR, circular dichroic spectroscopy, dielectric spectroscopy and SEM to explore the influence of LBG on collagen with respect to static and dynamic behaviour.
24528727	4	24	theme	hydration	880:888	arg1	dependent					903:911	dependent	903:911	dependent	903:911	The relaxation process of collagen in the frequency range of 0.01 Hz to 10(5)Hz and thermoporometry results indicate that the interfacial hydration dynamics are dependent on the applied concentration of LBG.
24528727	4	24	theme	hydration	880:888	arg1	dynamics					890:897	the interfacial hydration dynamics	864:897	the interfacial hydration dynamics	864:897	The relaxation process of collagen in the frequency range of 0.01 Hz to 10(5)Hz and thermoporometry results indicate that the interfacial hydration dynamics are dependent on the applied concentration of LBG.
24528727	5	25	theme	structural	1015:1024	arg1	clusters					1032:1039	the structural water clusters	1011:1039	the structural water clusters around the charged amino acids of collagen	1011:1082	This investigation explicitly reflects the rearrangements of the structural water clusters around the charged amino acids of collagen.
24528727	0	26	theme	hydration	15:23	arg1	dynamics					25:32	hydration dynamics	15:32	hydration dynamics of locust bean gum-collagen composites	15:71	Elucidation of hydration dynamics of locust bean gum-collagen composites by impedance and thermoporometry.
24528727	4	27	theme	interfacial	868:878	arg1	dependent					903:911	dependent	903:911	dependent	903:911	The relaxation process of collagen in the frequency range of 0.01 Hz to 10(5)Hz and thermoporometry results indicate that the interfacial hydration dynamics are dependent on the applied concentration of LBG.
24528727	4	27	theme	interfacial	868:878	arg1	dynamics					890:897	the interfacial hydration dynamics	864:897	the interfacial hydration dynamics	864:897	The relaxation process of collagen in the frequency range of 0.01 Hz to 10(5)Hz and thermoporometry results indicate that the interfacial hydration dynamics are dependent on the applied concentration of LBG.
24528727	3	28	theme	liquid	491:496	arg1	probe					485:489	a probe	483:489	a probe liquid	483:496	Water, as a probe liquid bound in collagen has been investigated using a combination of thermoporometry, ATR-FTIR, circular dichroic spectroscopy, dielectric spectroscopy and SEM to explore the influence of LBG on collagen with respect to static and dynamic behaviour.
24528727	2	29	theme	biopolymers	404:414	arg1	dynamics					388:395	solvation dynamics	378:395	solvation dynamics of the biopolymers affecting the surface as well as interfacial properties	378:470	This work presents the molecular motions of collagen originating from the solvents and locust bean gum (LBG), which reveal the changes in solvation dynamics of the biopolymers affecting the surface as well as interfacial properties.
24528727	0	30	theme	locust	37:42	arg1	composites					62:71	locust bean gum-collagen composites	37:71	locust bean gum-collagen composites	37:71	Elucidation of hydration dynamics of locust bean gum-collagen composites by impedance and thermoporometry.
24528727	3	31	theme	dynamic	723:729	arg1	behaviour					731:739	static and dynamic behaviour	712:739	static and dynamic behaviour	712:739	Water, as a probe liquid bound in collagen has been investigated using a combination of thermoporometry, ATR-FTIR, circular dichroic spectroscopy, dielectric spectroscopy and SEM to explore the influence of LBG on collagen with respect to static and dynamic behaviour.
24528727	3	32	theme	SEM	648:650	arg1	combination					546:556	a combination	544:556	a combination of thermoporometry, ATR-FTIR, circular dichroic spectroscopy, dielectric spectroscopy and SEM	544:650	Water, as a probe liquid bound in collagen has been investigated using a combination of thermoporometry, ATR-FTIR, circular dichroic spectroscopy, dielectric spectroscopy and SEM to explore the influence of LBG on collagen with respect to static and dynamic behaviour.
24528727	3	33	theme	ATR-FTIR	578:585	arg1	spectroscopy					606:617	ATR-FTIR, circular dichroic spectroscopy	578:617	spectroscopy	606:617	Water, as a probe liquid bound in collagen has been investigated using a combination of thermoporometry, ATR-FTIR, circular dichroic spectroscopy, dielectric spectroscopy and SEM to explore the influence of LBG on collagen with respect to static and dynamic behaviour.
24528727	3	34	theme	dielectric	620:629	arg1	spectroscopy					631:642	dielectric spectroscopy	620:642	dielectric spectroscopy	620:642	Water, as a probe liquid bound in collagen has been investigated using a combination of thermoporometry, ATR-FTIR, circular dichroic spectroscopy, dielectric spectroscopy and SEM to explore the influence of LBG on collagen with respect to static and dynamic behaviour.
24528727	2	35	theme	interfacial	449:459	arg1	properties					461:470	interfacial properties	449:470	the surface as well as interfacial properties	426:470	This work presents the molecular motions of collagen originating from the solvents and locust bean gum (LBG), which reveal the changes in solvation dynamics of the biopolymers affecting the surface as well as interfacial properties.
24528727	0	36	theme	composites	62:71	arg1	dynamics					25:32	hydration dynamics	15:32	hydration dynamics of locust bean gum-collagen composites	15:71	Elucidation of hydration dynamics of locust bean gum-collagen composites by impedance and thermoporometry.
24528727	3	37	theme	thermoporometry	561:575	arg1	combination					546:556	a combination	544:556	a combination of thermoporometry, ATR-FTIR, circular dichroic spectroscopy, dielectric spectroscopy and SEM	544:650	Water, as a probe liquid bound in collagen has been investigated using a combination of thermoporometry, ATR-FTIR, circular dichroic spectroscopy, dielectric spectroscopy and SEM to explore the influence of LBG on collagen with respect to static and dynamic behaviour.
24528727	2	38	from	changes	367:373	arg1	dynamics					388:395	solvation dynamics	378:395	solvation dynamics of the biopolymers affecting the surface as well as interfacial properties	378:470	This work presents the molecular motions of collagen originating from the solvents and locust bean gum (LBG), which reveal the changes in solvation dynamics of the biopolymers affecting the surface as well as interfacial properties.
24528727	0	39	theme	bean	44:47	arg1	composites					62:71	locust bean gum-collagen composites	37:71	locust bean gum-collagen composites	37:71	Elucidation of hydration dynamics of locust bean gum-collagen composites by impedance and thermoporometry.
24528727	3	40	theme	spectroscopy	631:642	arg1	combination					546:556	a combination	544:556	a combination of thermoporometry, ATR-FTIR, circular dichroic spectroscopy, dielectric spectroscopy and SEM	544:650	Water, as a probe liquid bound in collagen has been investigated using a combination of thermoporometry, ATR-FTIR, circular dichroic spectroscopy, dielectric spectroscopy and SEM to explore the influence of LBG on collagen with respect to static and dynamic behaviour.
24528727	2	41	dep	solvents	314:321	arg1	the					310:312	the	310:312	the	310:312	This work presents the molecular motions of collagen originating from the solvents and locust bean gum (LBG), which reveal the changes in solvation dynamics of the biopolymers affecting the surface as well as interfacial properties.
24528727	6	42	theme	collagen	1167:1174	arg1	biomaterials					1182:1193	collagen based biomaterials	1167:1193	collagen based biomaterials	1167:1193	These results can be employed to redesign the approach towards the development of collagen based biomaterials.
24528727	5	43	theme	water	1026:1030	arg1	clusters					1032:1039	the structural water clusters	1011:1039	the structural water clusters around the charged amino acids of collagen	1011:1082	This investigation explicitly reflects the rearrangements of the structural water clusters around the charged amino acids of collagen.
24528727	3	44	theme	spectroscopy	606:617	arg1	combination					546:556	a combination	544:556	a combination of thermoporometry, ATR-FTIR, circular dichroic spectroscopy, dielectric spectroscopy and SEM	544:650	Water, as a probe liquid bound in collagen has been investigated using a combination of thermoporometry, ATR-FTIR, circular dichroic spectroscopy, dielectric spectroscopy and SEM to explore the influence of LBG on collagen with respect to static and dynamic behaviour.
24528727	4	45	from	process	757:763	arg1	range					794:798	the frequency range	780:798	the frequency range of 0.01 Hz to 10(5)Hz	780:820	The relaxation process of collagen in the frequency range of 0.01 Hz to 10(5)Hz and thermoporometry results indicate that the interfacial hydration dynamics are dependent on the applied concentration of LBG.
24528727	1	46	theme	different	128:136	arg1	parameters					138:147	the different parameters	124:147	the different parameters involved in the hydration dynamics of collagen	124:194	The intricacy of the different parameters involved in the hydration dynamics of collagen influences its performance as biomaterials.
24528727	4	47	theme	process	757:763	arg1	results					842:848	The relaxation process of collagen in the frequency range of 0.01 Hz to 10(5)Hz and thermoporometry results	742:848	The relaxation process of collagen in the frequency range of 0.01 Hz to 10(5)Hz and thermoporometry results	742:848	The relaxation process of collagen in the frequency range of 0.01 Hz to 10(5)Hz and thermoporometry results indicate that the interfacial hydration dynamics are dependent on the applied concentration of LBG.
24528727	5	48	theme	clusters	1032:1039	arg1	rearrangements					993:1006	the rearrangements	989:1006	the rearrangements of the structural water clusters around the charged amino acids of collagen	989:1082	This investigation explicitly reflects the rearrangements of the structural water clusters around the charged amino acids of collagen.
24528727	4	49	theme	collagen	768:775	arg1	process					757:763	The relaxation process	742:763	The relaxation process of collagen in the frequency range of 0.01 Hz to 10(5)Hz	742:820	The relaxation process of collagen in the frequency range of 0.01 Hz to 10(5)Hz and thermoporometry results indicate that the interfacial hydration dynamics are dependent on the applied concentration of LBG.
24528727	1	50	theme	parameters	138:147	arg1	intricacy					111:119	The intricacy	107:119	The intricacy of the different parameters involved in the hydration dynamics of collagen	107:194	The intricacy of the different parameters involved in the hydration dynamics of collagen influences its performance as biomaterials.
24528727	2	51	theme	molecular	263:271	arg1	motions					273:279	the molecular motions	259:279	the molecular motions of collagen originating from the solvents and locust bean gum (LBG), which reveal the changes in solvation dynamics of the biopolymers affecting the surface as well as interfacial properties	259:470	This work presents the molecular motions of collagen originating from the solvents and locust bean gum (LBG), which reveal the changes in solvation dynamics of the biopolymers affecting the surface as well as interfacial properties.
24528727	0	52	theme	gum-collagen	49:60	arg1	composites					62:71	locust bean gum-collagen composites	37:71	locust bean gum-collagen composites	37:71	Elucidation of hydration dynamics of locust bean gum-collagen composites by impedance and thermoporometry.
24528727	3	53	theme	dichroic	597:604	arg1	spectroscopy					606:617	ATR-FTIR, circular dichroic spectroscopy	578:617	spectroscopy	606:617	Water, as a probe liquid bound in collagen has been investigated using a combination of thermoporometry, ATR-FTIR, circular dichroic spectroscopy, dielectric spectroscopy and SEM to explore the influence of LBG on collagen with respect to static and dynamic behaviour.
24528727	4	54	dep	Hz	819:820	arg1	to					811:812	to	811:812	to	811:812	The relaxation process of collagen in the frequency range of 0.01 Hz to 10(5)Hz and thermoporometry results indicate that the interfacial hydration dynamics are dependent on the applied concentration of LBG.
27865619	11	0	theme	c-caspase	1619:1627	arg1	pathway					1629:1635	p53-mediated cytochrome c-caspase pathway	1595:1635	p53-mediated cytochrome c-caspase pathway	1595:1635	In vitro studies showed that treatment of triple negative breast cancer (TNBC) MDA-MB-231 cells with Se-PFPs (1) inhibited cell growth dose-dependently by arresting cells at G2 phase via inhibiting CDC25C-CyclinB1/CDC2 pathway; (2) caused apoptosis associated with increased p53, Bax, Puma and Noxa, decreased Bcl2, increased Bax/Bcl2 ratio and increased activities of caspases 3/9, suggesting its effect on p53-mediated cytochrome c-caspase pathway.
27865619	0	1	theme	Pyracantha	156:165	arg1	fortuneana					167:176	Pyracantha fortuneana	156:176	Pyracantha fortuneana	156:176	Inhibition on the growth of human MDA-MB-231 breast cancer cells in vitro and tumor growth in a mouse xenograft model by Se-containing polysaccharides from Pyracantha fortuneana.
27865619	11	2	dep	caspases	1556:1563	arg1	3/9					1565:1567	3/9	1565:1567	caspases 3/9	1556:1567	In vitro studies showed that treatment of triple negative breast cancer (TNBC) MDA-MB-231 cells with Se-PFPs (1) inhibited cell growth dose-dependently by arresting cells at G2 phase via inhibiting CDC25C-CyclinB1/CDC2 pathway; (2) caused apoptosis associated with increased p53, Bax, Puma and Noxa, decreased Bcl2, increased Bax/Bcl2 ratio and increased activities of caspases 3/9, suggesting its effect on p53-mediated cytochrome c-caspase pathway.
27865619	11	3	theme	increased	1503:1511	arg1	ratio					1522:1526	increased Bax/Bcl2 ratio	1503:1526	increased Bax/Bcl2 ratio	1503:1526	In vitro studies showed that treatment of triple negative breast cancer (TNBC) MDA-MB-231 cells with Se-PFPs (1) inhibited cell growth dose-dependently by arresting cells at G2 phase via inhibiting CDC25C-CyclinB1/CDC2 pathway; (2) caused apoptosis associated with increased p53, Bax, Puma and Noxa, decreased Bcl2, increased Bax/Bcl2 ratio and increased activities of caspases 3/9, suggesting its effect on p53-mediated cytochrome c-caspase pathway.
27865619	2	4	theme	breast	272:277	arg1	cancer					279:284	breast cancer	272:284	breast cancer	272:284	Current therapies for breast cancer have adverse side-effects.
27865619	14	5	from	fortuneana	1952:1961	arg1	polysaccharides					1928:1942	Se-containing polysaccharides	1914:1942	Se-containing polysaccharides from P. fortuneana	1914:1961	It is concluded that Se-containing polysaccharides from P. fortuneana potently inhibit the growth and induce apoptosis of TNBC cells and can be potential anticancer agent for TNBC.
27865619	10	6	contain	contained	1040:1048	arg2	w/w					1085:1087	w/w	1085:1087	w/w	1085:1087	This extract contained 93.7% (w/w) of carbohydrate, 2.1% (w/w) of uronic acid and 3.7μg/g of Se, and was considered as Se-conjugated polysaccharides (Se-PFPs).
27865619	10	6	contain	contained	1040:1048	arg2	3.7μg/g					1109:1115	3.7μg/g	1109:1115	3.7μg/g of Se	1109:1121	This extract contained 93.7% (w/w) of carbohydrate, 2.1% (w/w) of uronic acid and 3.7μg/g of Se, and was considered as Se-conjugated polysaccharides (Se-PFPs).
27865619	10	6	contain	contained	1040:1048	arg1	polysaccharides					1160:1174	Se-conjugated polysaccharides	1146:1174	Se-conjugated polysaccharides (Se-PFPs)	1146:1184	This extract contained 93.7% (w/w) of carbohydrate, 2.1% (w/w) of uronic acid and 3.7μg/g of Se, and was considered as Se-conjugated polysaccharides (Se-PFPs).
27865619	10	6	contain	contained	1040:1048	arg2	Se					1120:1121	Se	1120:1121	Se	1120:1121	This extract contained 93.7% (w/w) of carbohydrate, 2.1% (w/w) of uronic acid and 3.7μg/g of Se, and was considered as Se-conjugated polysaccharides (Se-PFPs).
27865619	10	6	contain	contained	1040:1048	arg2	%					1054:1054	93.7%	1050:1054	93.7% (w/w) of carbohydrate	1050:1076	This extract contained 93.7% (w/w) of carbohydrate, 2.1% (w/w) of uronic acid and 3.7μg/g of Se, and was considered as Se-conjugated polysaccharides (Se-PFPs).
27865619	10	6	contain	contained	1040:1048	arg2	carbohydrate					1065:1076	carbohydrate	1065:1076	carbohydrate	1065:1076	This extract contained 93.7% (w/w) of carbohydrate, 2.1% (w/w) of uronic acid and 3.7μg/g of Se, and was considered as Se-conjugated polysaccharides (Se-PFPs).
27865619	10	6	contain	contained	1040:1048	arg1	extract					1032:1038	This extract	1027:1038	This extract	1027:1038	This extract contained 93.7% (w/w) of carbohydrate, 2.1% (w/w) of uronic acid and 3.7μg/g of Se, and was considered as Se-conjugated polysaccharides (Se-PFPs).
27865619	10	6	contain	contained	1040:1048	arg2	%					1082:1082	2.1%	1079:1082	2.1% (w/w) of uronic acid	1079:1103	This extract contained 93.7% (w/w) of carbohydrate, 2.1% (w/w) of uronic acid and 3.7μg/g of Se, and was considered as Se-conjugated polysaccharides (Se-PFPs).
27865619	10	6	contain	contained	1040:1048	arg2	w/w					1057:1059	w/w	1057:1059	w/w	1057:1059	This extract contained 93.7% (w/w) of carbohydrate, 2.1% (w/w) of uronic acid and 3.7μg/g of Se, and was considered as Se-conjugated polysaccharides (Se-PFPs).
27865619	10	6	contain	contained	1040:1048	arg2	acid					1100:1103	uronic acid	1093:1103	uronic acid	1093:1103	This extract contained 93.7% (w/w) of carbohydrate, 2.1% (w/w) of uronic acid and 3.7μg/g of Se, and was considered as Se-conjugated polysaccharides (Se-PFPs).
27865619	11	7	theme	increased	1532:1540	arg1	activities					1542:1551	increased activities	1532:1551	increased activities of caspases 3/9	1532:1567	In vitro studies showed that treatment of triple negative breast cancer (TNBC) MDA-MB-231 cells with Se-PFPs (1) inhibited cell growth dose-dependently by arresting cells at G2 phase via inhibiting CDC25C-CyclinB1/CDC2 pathway; (2) caused apoptosis associated with increased p53, Bax, Puma and Noxa, decreased Bcl2, increased Bax/Bcl2 ratio and increased activities of caspases 3/9, suggesting its effect on p53-mediated cytochrome c-caspase pathway.
27865619	1	8	theme	Breast	179:184	arg1	cause					207:211	the second cause	196:211	the second cause of cancer-related death among Women	196:247	Breast cancer is the second cause of cancer-related death among Women.
27865619	1	8	theme	Breast	179:184	arg1	cancer					186:191	Breast cancer	179:191	Breast cancer	179:191	Breast cancer is the second cause of cancer-related death among Women.
27865619	11	9	with	treatment	1216:1224	arg1	Se-PFPs					1288:1294	Se-PFPs (1)	1288:1298	Se-PFPs (1)	1288:1298	In vitro studies showed that treatment of triple negative breast cancer (TNBC) MDA-MB-231 cells with Se-PFPs (1) inhibited cell growth dose-dependently by arresting cells at G2 phase via inhibiting CDC25C-CyclinB1/CDC2 pathway; (2) caused apoptosis associated with increased p53, Bax, Puma and Noxa, decreased Bcl2, increased Bax/Bcl2 ratio and increased activities of caspases 3/9, suggesting its effect on p53-mediated cytochrome c-caspase pathway.
27865619	4	10	theme	rich	442:445	arg1	polysaccharides					450:464	rich Se polysaccharides	442:464	rich Se polysaccharides	442:464	Pyracantha fortuneana (P. fortuneana) contains rich Se polysaccharides.
27865619	0	11	theme	xenograft	102:110	arg1	model					112:116	a mouse xenograft model	94:116	a mouse xenograft model	94:116	Inhibition on the growth of human MDA-MB-231 breast cancer cells in vitro and tumor growth in a mouse xenograft model by Se-containing polysaccharides from Pyracantha fortuneana.
27865619	5	12	theme	breast	568:573	arg1	cancer					575:580	breast cancer	568:580	breast cancer	568:580	We hypothesized that Se-containing polysaccharides from P. fortuneana possess anticancer activity on breast cancer via inhibiting growth and inducing apoptosis.
27865619	1	13	theme	second	200:205	arg1	cause					207:211	the second cause	196:211	the second cause of cancer-related death among Women	196:247	Breast cancer is the second cause of cancer-related death among Women.
27865619	1	13	theme	second	200:205	arg1	cancer					186:191	Breast cancer	179:191	Breast cancer	179:191	Breast cancer is the second cause of cancer-related death among Women.
27865619	9	14	theme	apoptosis	929:937	arg1	expression					899:908	expression	899:908	expression of cycle proteins, apoptosis and apoptosis-related protein	899:967	Their effects on cell growth, expression of cycle proteins, apoptosis and apoptosis-related protein, and tumor growth in mouse xenograft model were examined.
27865619	9	14	theme	apoptosis	929:937	arg1	growth					891:896	cell growth	886:896	cell growth	886:896	Their effects on cell growth, expression of cycle proteins, apoptosis and apoptosis-related protein, and tumor growth in mouse xenograft model were examined.
27865619	9	14	theme	apoptosis	929:937	arg1	growth					980:985	tumor growth	974:985	tumor growth in mouse xenograft model	974:1010	Their effects on cell growth, expression of cycle proteins, apoptosis and apoptosis-related protein, and tumor growth in mouse xenograft model were examined.
27865619	0	15	from	growth	84:89	arg1	model					112:116	a mouse xenograft model	94:116	a mouse xenograft model	94:116	Inhibition on the growth of human MDA-MB-231 breast cancer cells in vitro and tumor growth in a mouse xenograft model by Se-containing polysaccharides from Pyracantha fortuneana.
27865619	0	15	from	growth	84:89	arg1	growth					18:23	the growth	14:23	the growth of human MDA-MB-231 breast cancer cells	14:63	Inhibition on the growth of human MDA-MB-231 breast cancer cells in vitro and tumor growth in a mouse xenograft model by Se-containing polysaccharides from Pyracantha fortuneana.
27865619	6	16	theme	Se-containing	680:692	arg1	polysaccharides					694:708	Se-containing polysaccharides	680:708	Se-containing polysaccharides from P. fortuneana and the underlying mechanisms	680:757	This study aimed to assess the anticancer effect of Se-containing polysaccharides from P. fortuneana and the underlying mechanisms.
27865619	12	17	link	MDA-MB-231-derived	1669:1686	arg1	tumors					1698:1703	MDA-MB-231-derived xenograft tumors	1669:1703	MDA-MB-231-derived xenograft tumors	1669:1703	Treatment of nude mice bearing MDA-MB-231-derived xenograft tumors with Se-PFPs significantly reduced tumor growth without altering body weight, confirming its antitumor activity without toxic side effects.
27865619	12	18	theme	tumor	1740:1744	arg1	growth					1746:1751	tumor growth	1740:1751	tumor growth	1740:1751	Treatment of nude mice bearing MDA-MB-231-derived xenograft tumors with Se-PFPs significantly reduced tumor growth without altering body weight, confirming its antitumor activity without toxic side effects.
27865619	0	19	theme	cancer	52:57	arg1	cells					59:63	human MDA-MB-231 breast cancer cells	28:63	human MDA-MB-231 breast cancer cells	28:63	Inhibition on the growth of human MDA-MB-231 breast cancer cells in vitro and tumor growth in a mouse xenograft model by Se-containing polysaccharides from Pyracantha fortuneana.
27865619	12	20	theme	xenograft	1688:1696	arg1	tumors					1698:1703	MDA-MB-231-derived xenograft tumors	1669:1703	MDA-MB-231-derived xenograft tumors	1669:1703	Treatment of nude mice bearing MDA-MB-231-derived xenograft tumors with Se-PFPs significantly reduced tumor growth without altering body weight, confirming its antitumor activity without toxic side effects.
27865619	3	21	theme	Selenium	313:320	arg1	polysaccharides					338:352	Selenium (Se)-containing polysaccharides	313:352	Selenium (Se)-containing polysaccharides	313:352	Selenium (Se)-containing polysaccharides have multiple health benefits to humans.
27865619	9	22	theme	cell	886:889	arg1	growth					891:896	cell growth	886:896	cell growth	886:896	Their effects on cell growth, expression of cycle proteins, apoptosis and apoptosis-related protein, and tumor growth in mouse xenograft model were examined.
27865619	4	23	dep	fortuneana	406:415	arg1	fortuneana					421:430	P. fortuneana	418:430	P. fortuneana	418:430	Pyracantha fortuneana (P. fortuneana) contains rich Se polysaccharides.
27865619	10	24	theme	acid	1100:1103	arg1	Se					1120:1121	Se	1120:1121	Se	1120:1121	This extract contained 93.7% (w/w) of carbohydrate, 2.1% (w/w) of uronic acid and 3.7μg/g of Se, and was considered as Se-conjugated polysaccharides (Se-PFPs).
27865619	10	24	theme	acid	1100:1103	arg1	carbohydrate					1065:1076	carbohydrate	1065:1076	carbohydrate	1065:1076	This extract contained 93.7% (w/w) of carbohydrate, 2.1% (w/w) of uronic acid and 3.7μg/g of Se, and was considered as Se-conjugated polysaccharides (Se-PFPs).
27865619	10	24	theme	acid	1100:1103	arg1	%					1054:1054	93.7%	1050:1054	93.7% (w/w) of carbohydrate	1050:1076	This extract contained 93.7% (w/w) of carbohydrate, 2.1% (w/w) of uronic acid and 3.7μg/g of Se, and was considered as Se-conjugated polysaccharides (Se-PFPs).
27865619	10	24	theme	acid	1100:1103	arg1	%					1082:1082	2.1%	1079:1082	2.1% (w/w) of uronic acid	1079:1103	This extract contained 93.7% (w/w) of carbohydrate, 2.1% (w/w) of uronic acid and 3.7μg/g of Se, and was considered as Se-conjugated polysaccharides (Se-PFPs).
27865619	10	24	theme	acid	1100:1103	arg1	acid					1100:1103	uronic acid	1093:1103	uronic acid	1093:1103	This extract contained 93.7% (w/w) of carbohydrate, 2.1% (w/w) of uronic acid and 3.7μg/g of Se, and was considered as Se-conjugated polysaccharides (Se-PFPs).
27865619	10	24	theme	acid	1100:1103	arg1	w/w					1057:1059	w/w	1057:1059	w/w	1057:1059	This extract contained 93.7% (w/w) of carbohydrate, 2.1% (w/w) of uronic acid and 3.7μg/g of Se, and was considered as Se-conjugated polysaccharides (Se-PFPs).
27865619	10	24	theme	acid	1100:1103	arg1	3.7μg/g					1109:1115	3.7μg/g	1109:1115	3.7μg/g of Se	1109:1121	This extract contained 93.7% (w/w) of carbohydrate, 2.1% (w/w) of uronic acid and 3.7μg/g of Se, and was considered as Se-conjugated polysaccharides (Se-PFPs).
27865619	10	24	theme	acid	1100:1103	arg1	w/w					1085:1087	w/w	1085:1087	w/w	1085:1087	This extract contained 93.7% (w/w) of carbohydrate, 2.1% (w/w) of uronic acid and 3.7μg/g of Se, and was considered as Se-conjugated polysaccharides (Se-PFPs).
27865619	0	25	from	Inhibition	0:9	arg1	model					112:116	a mouse xenograft model	94:116	a mouse xenograft model	94:116	Inhibition on the growth of human MDA-MB-231 breast cancer cells in vitro and tumor growth in a mouse xenograft model by Se-containing polysaccharides from Pyracantha fortuneana.
27865619	0	25	from	Inhibition	0:9	arg1	growth					18:23	the growth	14:23	the growth of human MDA-MB-231 breast cancer cells	14:63	Inhibition on the growth of human MDA-MB-231 breast cancer cells in vitro and tumor growth in a mouse xenograft model by Se-containing polysaccharides from Pyracantha fortuneana.
27865619	11	26	theme	cells	1277:1281	arg1	treatment					1216:1224	treatment	1216:1224	treatment of triple negative breast cancer (TNBC) MDA-MB-231 cells with Se-PFPs (1)	1216:1298	In vitro studies showed that treatment of triple negative breast cancer (TNBC) MDA-MB-231 cells with Se-PFPs (1) inhibited cell growth dose-dependently by arresting cells at G2 phase via inhibiting CDC25C-CyclinB1/CDC2 pathway; (2) caused apoptosis associated with increased p53, Bax, Puma and Noxa, decreased Bcl2, increased Bax/Bcl2 ratio and increased activities of caspases 3/9, suggesting its effect on p53-mediated cytochrome c-caspase pathway.
27865619	13	27	theme	cytotoxic	1874:1882	arg1	effects					1884:1890	doxorubicin cytotoxic effects	1862:1890	doxorubicin cytotoxic effects	1862:1890	Se-PFPs enhanced doxorubicin cytotoxic effects.
27865619	12	28	theme	nude	1651:1654	arg1	mice					1656:1659	nude mice	1651:1659	nude mice bearing MDA-MB-231-derived xenograft tumors	1651:1703	Treatment of nude mice bearing MDA-MB-231-derived xenograft tumors with Se-PFPs significantly reduced tumor growth without altering body weight, confirming its antitumor activity without toxic side effects.
27865619	3	29	theme	-containing	326:336	arg1	polysaccharides					338:352	Selenium (Se)-containing polysaccharides	313:352	Selenium (Se)-containing polysaccharides	313:352	Selenium (Se)-containing polysaccharides have multiple health benefits to humans.
27865619	10	30	theme	Se	1120:1121	arg1	Se					1120:1121	Se	1120:1121	Se	1120:1121	This extract contained 93.7% (w/w) of carbohydrate, 2.1% (w/w) of uronic acid and 3.7μg/g of Se, and was considered as Se-conjugated polysaccharides (Se-PFPs).
27865619	10	30	theme	Se	1120:1121	arg1	carbohydrate					1065:1076	carbohydrate	1065:1076	carbohydrate	1065:1076	This extract contained 93.7% (w/w) of carbohydrate, 2.1% (w/w) of uronic acid and 3.7μg/g of Se, and was considered as Se-conjugated polysaccharides (Se-PFPs).
27865619	10	30	theme	Se	1120:1121	arg1	%					1054:1054	93.7%	1050:1054	93.7% (w/w) of carbohydrate	1050:1076	This extract contained 93.7% (w/w) of carbohydrate, 2.1% (w/w) of uronic acid and 3.7μg/g of Se, and was considered as Se-conjugated polysaccharides (Se-PFPs).
27865619	10	30	theme	Se	1120:1121	arg1	%					1082:1082	2.1%	1079:1082	2.1% (w/w) of uronic acid	1079:1103	This extract contained 93.7% (w/w) of carbohydrate, 2.1% (w/w) of uronic acid and 3.7μg/g of Se, and was considered as Se-conjugated polysaccharides (Se-PFPs).
27865619	10	30	theme	Se	1120:1121	arg1	acid					1100:1103	uronic acid	1093:1103	uronic acid	1093:1103	This extract contained 93.7% (w/w) of carbohydrate, 2.1% (w/w) of uronic acid and 3.7μg/g of Se, and was considered as Se-conjugated polysaccharides (Se-PFPs).
27865619	10	30	theme	Se	1120:1121	arg1	w/w					1057:1059	w/w	1057:1059	w/w	1057:1059	This extract contained 93.7% (w/w) of carbohydrate, 2.1% (w/w) of uronic acid and 3.7μg/g of Se, and was considered as Se-conjugated polysaccharides (Se-PFPs).
27865619	10	30	theme	Se	1120:1121	arg1	3.7μg/g					1109:1115	3.7μg/g	1109:1115	3.7μg/g of Se	1109:1121	This extract contained 93.7% (w/w) of carbohydrate, 2.1% (w/w) of uronic acid and 3.7μg/g of Se, and was considered as Se-conjugated polysaccharides (Se-PFPs).
27865619	10	30	theme	Se	1120:1121	arg1	w/w					1085:1087	w/w	1085:1087	w/w	1085:1087	This extract contained 93.7% (w/w) of carbohydrate, 2.1% (w/w) of uronic acid and 3.7μg/g of Se, and was considered as Se-conjugated polysaccharides (Se-PFPs).
27865619	14	31	theme	anticancer	2047:2056	arg1	agent					2058:2062	potential anticancer agent	2037:2062	potential anticancer agent for TNBC	2037:2071	It is concluded that Se-containing polysaccharides from P. fortuneana potently inhibit the growth and induce apoptosis of TNBC cells and can be potential anticancer agent for TNBC.
27865619	9	32	theme	proteins	919:926	arg1	expression					899:908	expression	899:908	expression of cycle proteins, apoptosis and apoptosis-related protein	899:967	Their effects on cell growth, expression of cycle proteins, apoptosis and apoptosis-related protein, and tumor growth in mouse xenograft model were examined.
27865619	9	32	theme	proteins	919:926	arg1	growth					891:896	cell growth	886:896	cell growth	886:896	Their effects on cell growth, expression of cycle proteins, apoptosis and apoptosis-related protein, and tumor growth in mouse xenograft model were examined.
27865619	9	32	theme	proteins	919:926	arg1	growth					980:985	tumor growth	974:985	tumor growth in mouse xenograft model	974:1010	Their effects on cell growth, expression of cycle proteins, apoptosis and apoptosis-related protein, and tumor growth in mouse xenograft model were examined.
27865619	11	33	theme	triple	1229:1234	arg1	cells					1277:1281	triple negative breast cancer (TNBC) MDA-MB-231 cells	1229:1281	triple negative breast cancer (TNBC) MDA-MB-231 cells	1229:1281	In vitro studies showed that treatment of triple negative breast cancer (TNBC) MDA-MB-231 cells with Se-PFPs (1) inhibited cell growth dose-dependently by arresting cells at G2 phase via inhibiting CDC25C-CyclinB1/CDC2 pathway; (2) caused apoptosis associated with increased p53, Bax, Puma and Noxa, decreased Bcl2, increased Bax/Bcl2 ratio and increased activities of caspases 3/9, suggesting its effect on p53-mediated cytochrome c-caspase pathway.
27865619	10	34	theme	Se-conjugated	1146:1158	arg1	extract					1032:1038	This extract	1027:1038	This extract	1027:1038	This extract contained 93.7% (w/w) of carbohydrate, 2.1% (w/w) of uronic acid and 3.7μg/g of Se, and was considered as Se-conjugated polysaccharides (Se-PFPs).
27865619	10	34	theme	Se-conjugated	1146:1158	arg1	Se-PFPs					1177:1183	Se-PFPs	1177:1183	Se-PFPs	1177:1183	This extract contained 93.7% (w/w) of carbohydrate, 2.1% (w/w) of uronic acid and 3.7μg/g of Se, and was considered as Se-conjugated polysaccharides (Se-PFPs).
27865619	10	34	theme	Se-conjugated	1146:1158	arg1	polysaccharides					1160:1174	Se-conjugated polysaccharides	1146:1174	Se-conjugated polysaccharides (Se-PFPs)	1146:1184	This extract contained 93.7% (w/w) of carbohydrate, 2.1% (w/w) of uronic acid and 3.7μg/g of Se, and was considered as Se-conjugated polysaccharides (Se-PFPs).
27865619	3	35	theme	health	368:373	arg1	benefits					375:382	multiple health benefits	359:382	multiple health benefits	359:382	Selenium (Se)-containing polysaccharides have multiple health benefits to humans.
27865619	11	36	theme	breast	1245:1250	arg1	cells					1277:1281	triple negative breast cancer (TNBC) MDA-MB-231 cells	1229:1281	triple negative breast cancer (TNBC) MDA-MB-231 cells	1229:1281	In vitro studies showed that treatment of triple negative breast cancer (TNBC) MDA-MB-231 cells with Se-PFPs (1) inhibited cell growth dose-dependently by arresting cells at G2 phase via inhibiting CDC25C-CyclinB1/CDC2 pathway; (2) caused apoptosis associated with increased p53, Bax, Puma and Noxa, decreased Bcl2, increased Bax/Bcl2 ratio and increased activities of caspases 3/9, suggesting its effect on p53-mediated cytochrome c-caspase pathway.
27865619	13	37	theme	doxorubicin	1862:1872	arg1	effects					1884:1890	doxorubicin cytotoxic effects	1862:1890	doxorubicin cytotoxic effects	1862:1890	Se-PFPs enhanced doxorubicin cytotoxic effects.
27865619	9	38	theme	apoptosis-related	943:959	arg1	protein					961:967	apoptosis-related protein	943:967	apoptosis-related protein	943:967	Their effects on cell growth, expression of cycle proteins, apoptosis and apoptosis-related protein, and tumor growth in mouse xenograft model were examined.
27865619	11	39	from	effect	1585:1590	arg1	pathway					1629:1635	p53-mediated cytochrome c-caspase pathway	1595:1635	p53-mediated cytochrome c-caspase pathway	1595:1635	In vitro studies showed that treatment of triple negative breast cancer (TNBC) MDA-MB-231 cells with Se-PFPs (1) inhibited cell growth dose-dependently by arresting cells at G2 phase via inhibiting CDC25C-CyclinB1/CDC2 pathway; (2) caused apoptosis associated with increased p53, Bax, Puma and Noxa, decreased Bcl2, increased Bax/Bcl2 ratio and increased activities of caspases 3/9, suggesting its effect on p53-mediated cytochrome c-caspase pathway.
27865619	2	40	contain	have	286:289	arg1	therapies					258:266	Current therapies	250:266	Current therapies for breast cancer	250:284	Current therapies for breast cancer have adverse side-effects.
27865619	2	40	contain	have	286:289	arg2	side-effects					299:310	adverse side-effects	291:310	adverse side-effects	291:310	Current therapies for breast cancer have adverse side-effects.
27865619	14	41	theme	TNBC	2015:2018	arg1	cells					2020:2024	TNBC cells	2015:2024	TNBC cells	2015:2024	It is concluded that Se-containing polysaccharides from P. fortuneana potently inhibit the growth and induce apoptosis of TNBC cells and can be potential anticancer agent for TNBC.
27865619	9	42	from	expression	899:908	arg1	model					1006:1010	mouse xenograft model	990:1010	mouse xenograft model	990:1010	Their effects on cell growth, expression of cycle proteins, apoptosis and apoptosis-related protein, and tumor growth in mouse xenograft model were examined.
27865619	11	43	theme	negative	1236:1243	arg1	cells					1277:1281	triple negative breast cancer (TNBC) MDA-MB-231 cells	1229:1281	triple negative breast cancer (TNBC) MDA-MB-231 cells	1229:1281	In vitro studies showed that treatment of triple negative breast cancer (TNBC) MDA-MB-231 cells with Se-PFPs (1) inhibited cell growth dose-dependently by arresting cells at G2 phase via inhibiting CDC25C-CyclinB1/CDC2 pathway; (2) caused apoptosis associated with increased p53, Bax, Puma and Noxa, decreased Bcl2, increased Bax/Bcl2 ratio and increased activities of caspases 3/9, suggesting its effect on p53-mediated cytochrome c-caspase pathway.
27865619	9	44	from	growth	891:896	arg1	model					1006:1010	mouse xenograft model	990:1010	mouse xenograft model	990:1010	Their effects on cell growth, expression of cycle proteins, apoptosis and apoptosis-related protein, and tumor growth in mouse xenograft model were examined.
27865619	9	45	theme	tumor	974:978	arg1	growth					980:985	tumor growth	974:985	tumor growth in mouse xenograft model	974:1010	Their effects on cell growth, expression of cycle proteins, apoptosis and apoptosis-related protein, and tumor growth in mouse xenograft model were examined.
27865619	5	46	from	fortuneana	526:535	arg1	polysaccharides					502:516	Se-containing polysaccharides	488:516	Se-containing polysaccharides from P. fortuneana	488:535	We hypothesized that Se-containing polysaccharides from P. fortuneana possess anticancer activity on breast cancer via inhibiting growth and inducing apoptosis.
27865619	9	47	from	growth	980:985	arg1	model					1006:1010	mouse xenograft model	990:1010	mouse xenograft model	990:1010	Their effects on cell growth, expression of cycle proteins, apoptosis and apoptosis-related protein, and tumor growth in mouse xenograft model were examined.
27865619	11	48	theme	CDC25C-CyclinB1/CDC2	1385:1404	arg1	pathway					1406:1412	CDC25C-CyclinB1/CDC2 pathway	1385:1412	CDC25C-CyclinB1/CDC2 pathway	1385:1412	In vitro studies showed that treatment of triple negative breast cancer (TNBC) MDA-MB-231 cells with Se-PFPs (1) inhibited cell growth dose-dependently by arresting cells at G2 phase via inhibiting CDC25C-CyclinB1/CDC2 pathway; (2) caused apoptosis associated with increased p53, Bax, Puma and Noxa, decreased Bcl2, increased Bax/Bcl2 ratio and increased activities of caspases 3/9, suggesting its effect on p53-mediated cytochrome c-caspase pathway.
27865619	11	49	theme	cytochrome	1608:1617	arg1	pathway					1629:1635	p53-mediated cytochrome c-caspase pathway	1595:1635	p53-mediated cytochrome c-caspase pathway	1595:1635	In vitro studies showed that treatment of triple negative breast cancer (TNBC) MDA-MB-231 cells with Se-PFPs (1) inhibited cell growth dose-dependently by arresting cells at G2 phase via inhibiting CDC25C-CyclinB1/CDC2 pathway; (2) caused apoptosis associated with increased p53, Bax, Puma and Noxa, decreased Bcl2, increased Bax/Bcl2 ratio and increased activities of caspases 3/9, suggesting its effect on p53-mediated cytochrome c-caspase pathway.
27865619	1	50	theme	cancer-related	216:229	arg1	death					231:235	cancer-related death	216:235	cancer-related death among Women	216:247	Breast cancer is the second cause of cancer-related death among Women.
27865619	12	51	theme	toxic	1825:1829	arg1	effects					1836:1842	toxic side effects	1825:1842	toxic side effects	1825:1842	Treatment of nude mice bearing MDA-MB-231-derived xenograft tumors with Se-PFPs significantly reduced tumor growth without altering body weight, confirming its antitumor activity without toxic side effects.
27865619	9	52	theme	xenograft	996:1004	arg1	model					1006:1010	mouse xenograft model	990:1010	mouse xenograft model	990:1010	Their effects on cell growth, expression of cycle proteins, apoptosis and apoptosis-related protein, and tumor growth in mouse xenograft model were examined.
27865619	4	53	theme	Pyracantha	395:404	arg1	fortuneana					406:415	Pyracantha fortuneana	395:415	Pyracantha fortuneana (P. fortuneana)	395:431	Pyracantha fortuneana (P. fortuneana) contains rich Se polysaccharides.
27865619	7	54	theme	Se-containing	760:772	arg1	polysaccharides					774:788	Se-containing polysaccharides	760:788	Se-containing polysaccharides	760:788	Se-containing polysaccharides were purified.
27865619	9	55	from	effects	875:881	arg1	expression					899:908	expression	899:908	expression of cycle proteins, apoptosis and apoptosis-related protein	899:967	Their effects on cell growth, expression of cycle proteins, apoptosis and apoptosis-related protein, and tumor growth in mouse xenograft model were examined.
27865619	9	55	from	effects	875:881	arg1	growth					891:896	cell growth	886:896	cell growth	886:896	Their effects on cell growth, expression of cycle proteins, apoptosis and apoptosis-related protein, and tumor growth in mouse xenograft model were examined.
27865619	9	55	from	effects	875:881	arg1	growth					980:985	tumor growth	974:985	tumor growth in mouse xenograft model	974:1010	Their effects on cell growth, expression of cycle proteins, apoptosis and apoptosis-related protein, and tumor growth in mouse xenograft model were examined.
27865619	11	56	theme	Bax/Bcl2	1513:1520	arg1	ratio					1522:1526	increased Bax/Bcl2 ratio	1503:1526	increased Bax/Bcl2 ratio	1503:1526	In vitro studies showed that treatment of triple negative breast cancer (TNBC) MDA-MB-231 cells with Se-PFPs (1) inhibited cell growth dose-dependently by arresting cells at G2 phase via inhibiting CDC25C-CyclinB1/CDC2 pathway; (2) caused apoptosis associated with increased p53, Bax, Puma and Noxa, decreased Bcl2, increased Bax/Bcl2 ratio and increased activities of caspases 3/9, suggesting its effect on p53-mediated cytochrome c-caspase pathway.
27865619	5	57	from	activity	556:563	arg1	cancer					575:580	breast cancer	568:580	breast cancer	568:580	We hypothesized that Se-containing polysaccharides from P. fortuneana possess anticancer activity on breast cancer via inhibiting growth and inducing apoptosis.
27865619	0	58	theme	MDA-MB-231	34:43	arg1	cells					59:63	human MDA-MB-231 breast cancer cells	28:63	human MDA-MB-231 breast cancer cells	28:63	Inhibition on the growth of human MDA-MB-231 breast cancer cells in vitro and tumor growth in a mouse xenograft model by Se-containing polysaccharides from Pyracantha fortuneana.
27865619	6	59	from	mechanisms	748:757	arg1	effect					670:675	the anticancer effect	655:675	the anticancer effect of Se-containing polysaccharides from P. fortuneana and the underlying mechanisms	655:757	This study aimed to assess the anticancer effect of Se-containing polysaccharides from P. fortuneana and the underlying mechanisms.
27865619	6	59	from	mechanisms	748:757	arg1	polysaccharides					694:708	Se-containing polysaccharides	680:708	Se-containing polysaccharides from P. fortuneana and the underlying mechanisms	680:757	This study aimed to assess the anticancer effect of Se-containing polysaccharides from P. fortuneana and the underlying mechanisms.
27865619	11	60	theme	caspases	1556:1563	arg1	ratio					1522:1526	increased Bax/Bcl2 ratio	1503:1526	increased Bax/Bcl2 ratio	1503:1526	In vitro studies showed that treatment of triple negative breast cancer (TNBC) MDA-MB-231 cells with Se-PFPs (1) inhibited cell growth dose-dependently by arresting cells at G2 phase via inhibiting CDC25C-CyclinB1/CDC2 pathway; (2) caused apoptosis associated with increased p53, Bax, Puma and Noxa, decreased Bcl2, increased Bax/Bcl2 ratio and increased activities of caspases 3/9, suggesting its effect on p53-mediated cytochrome c-caspase pathway.
27865619	11	60	theme	caspases	1556:1563	arg1	activities					1542:1551	increased activities	1532:1551	increased activities of caspases 3/9	1532:1567	In vitro studies showed that treatment of triple negative breast cancer (TNBC) MDA-MB-231 cells with Se-PFPs (1) inhibited cell growth dose-dependently by arresting cells at G2 phase via inhibiting CDC25C-CyclinB1/CDC2 pathway; (2) caused apoptosis associated with increased p53, Bax, Puma and Noxa, decreased Bcl2, increased Bax/Bcl2 ratio and increased activities of caspases 3/9, suggesting its effect on p53-mediated cytochrome c-caspase pathway.
27865619	11	60	theme	caspases	1556:1563	arg1	Bcl2					1497:1500	Bcl2	1497:1500	Bcl2	1497:1500	In vitro studies showed that treatment of triple negative breast cancer (TNBC) MDA-MB-231 cells with Se-PFPs (1) inhibited cell growth dose-dependently by arresting cells at G2 phase via inhibiting CDC25C-CyclinB1/CDC2 pathway; (2) caused apoptosis associated with increased p53, Bax, Puma and Noxa, decreased Bcl2, increased Bax/Bcl2 ratio and increased activities of caspases 3/9, suggesting its effect on p53-mediated cytochrome c-caspase pathway.
27865619	12	61	theme	body	1770:1773	arg1	weight					1775:1780	body weight	1770:1780	body weight	1770:1780	Treatment of nude mice bearing MDA-MB-231-derived xenograft tumors with Se-PFPs significantly reduced tumor growth without altering body weight, confirming its antitumor activity without toxic side effects.
27865619	0	62	theme	mouse	96:100	arg1	model					112:116	a mouse xenograft model	94:116	a mouse xenograft model	94:116	Inhibition on the growth of human MDA-MB-231 breast cancer cells in vitro and tumor growth in a mouse xenograft model by Se-containing polysaccharides from Pyracantha fortuneana.
27865619	2	63	theme	adverse	291:297	arg1	side-effects					299:310	adverse side-effects	291:310	adverse side-effects	291:310	Current therapies for breast cancer have adverse side-effects.
27865619	4	64	theme	Se	447:448	arg1	polysaccharides					450:464	rich Se polysaccharides	442:464	rich Se polysaccharides	442:464	Pyracantha fortuneana (P. fortuneana) contains rich Se polysaccharides.
27865619	6	65	from	fortuneana	718:727	arg1	effect					670:675	the anticancer effect	655:675	the anticancer effect of Se-containing polysaccharides from P. fortuneana and the underlying mechanisms	655:757	This study aimed to assess the anticancer effect of Se-containing polysaccharides from P. fortuneana and the underlying mechanisms.
27865619	6	65	from	fortuneana	718:727	arg1	polysaccharides					694:708	Se-containing polysaccharides	680:708	Se-containing polysaccharides from P. fortuneana and the underlying mechanisms	680:757	This study aimed to assess the anticancer effect of Se-containing polysaccharides from P. fortuneana and the underlying mechanisms.
27865619	11	66	theme	G2	1361:1362	arg1	phase					1364:1368	G2 phase	1361:1368	G2 phase via inhibiting CDC25C-CyclinB1/CDC2 pathway	1361:1412	In vitro studies showed that treatment of triple negative breast cancer (TNBC) MDA-MB-231 cells with Se-PFPs (1) inhibited cell growth dose-dependently by arresting cells at G2 phase via inhibiting CDC25C-CyclinB1/CDC2 pathway; (2) caused apoptosis associated with increased p53, Bax, Puma and Noxa, decreased Bcl2, increased Bax/Bcl2 ratio and increased activities of caspases 3/9, suggesting its effect on p53-mediated cytochrome c-caspase pathway.
27865619	5	67	theme	Se-containing	488:500	arg1	polysaccharides					502:516	Se-containing polysaccharides	488:516	Se-containing polysaccharides from P. fortuneana	488:535	We hypothesized that Se-containing polysaccharides from P. fortuneana possess anticancer activity on breast cancer via inhibiting growth and inducing apoptosis.
27865619	14	68	theme	Se-containing	1914:1926	arg1	polysaccharides					1928:1942	Se-containing polysaccharides	1914:1942	Se-containing polysaccharides from P. fortuneana	1914:1961	It is concluded that Se-containing polysaccharides from P. fortuneana potently inhibit the growth and induce apoptosis of TNBC cells and can be potential anticancer agent for TNBC.
27865619	6	69	from	effect	670:675	arg1	fortuneana					718:727	P. fortuneana	715:727	P. fortuneana	715:727	This study aimed to assess the anticancer effect of Se-containing polysaccharides from P. fortuneana and the underlying mechanisms.
27865619	6	69	from	effect	670:675	arg1	mechanisms					748:757	the underlying mechanisms	733:757	the underlying mechanisms	733:757	This study aimed to assess the anticancer effect of Se-containing polysaccharides from P. fortuneana and the underlying mechanisms.
27865619	11	70	dep	caused	1419:1424	arg1	2					1416:1416	2	1416:1416	2	1416:1416	In vitro studies showed that treatment of triple negative breast cancer (TNBC) MDA-MB-231 cells with Se-PFPs (1) inhibited cell growth dose-dependently by arresting cells at G2 phase via inhibiting CDC25C-CyclinB1/CDC2 pathway; (2) caused apoptosis associated with increased p53, Bax, Puma and Noxa, decreased Bcl2, increased Bax/Bcl2 ratio and increased activities of caspases 3/9, suggesting its effect on p53-mediated cytochrome c-caspase pathway.
27865619	0	71	theme	Se-containing	121:133	arg1	polysaccharides					135:149	Se-containing polysaccharides	121:149	Se-containing polysaccharides from Pyracantha fortuneana	121:176	Inhibition on the growth of human MDA-MB-231 breast cancer cells in vitro and tumor growth in a mouse xenograft model by Se-containing polysaccharides from Pyracantha fortuneana.
27865619	10	72	theme	carbohydrate	1065:1076	arg1	Se					1120:1121	Se	1120:1121	Se	1120:1121	This extract contained 93.7% (w/w) of carbohydrate, 2.1% (w/w) of uronic acid and 3.7μg/g of Se, and was considered as Se-conjugated polysaccharides (Se-PFPs).
27865619	10	72	theme	carbohydrate	1065:1076	arg1	carbohydrate					1065:1076	carbohydrate	1065:1076	carbohydrate	1065:1076	This extract contained 93.7% (w/w) of carbohydrate, 2.1% (w/w) of uronic acid and 3.7μg/g of Se, and was considered as Se-conjugated polysaccharides (Se-PFPs).
27865619	10	72	theme	carbohydrate	1065:1076	arg1	%					1054:1054	93.7%	1050:1054	93.7% (w/w) of carbohydrate	1050:1076	This extract contained 93.7% (w/w) of carbohydrate, 2.1% (w/w) of uronic acid and 3.7μg/g of Se, and was considered as Se-conjugated polysaccharides (Se-PFPs).
27865619	10	72	theme	carbohydrate	1065:1076	arg1	%					1082:1082	2.1%	1079:1082	2.1% (w/w) of uronic acid	1079:1103	This extract contained 93.7% (w/w) of carbohydrate, 2.1% (w/w) of uronic acid and 3.7μg/g of Se, and was considered as Se-conjugated polysaccharides (Se-PFPs).
27865619	10	72	theme	carbohydrate	1065:1076	arg1	acid					1100:1103	uronic acid	1093:1103	uronic acid	1093:1103	This extract contained 93.7% (w/w) of carbohydrate, 2.1% (w/w) of uronic acid and 3.7μg/g of Se, and was considered as Se-conjugated polysaccharides (Se-PFPs).
27865619	10	72	theme	carbohydrate	1065:1076	arg1	w/w					1057:1059	w/w	1057:1059	w/w	1057:1059	This extract contained 93.7% (w/w) of carbohydrate, 2.1% (w/w) of uronic acid and 3.7μg/g of Se, and was considered as Se-conjugated polysaccharides (Se-PFPs).
27865619	10	72	theme	carbohydrate	1065:1076	arg1	3.7μg/g					1109:1115	3.7μg/g	1109:1115	3.7μg/g of Se	1109:1121	This extract contained 93.7% (w/w) of carbohydrate, 2.1% (w/w) of uronic acid and 3.7μg/g of Se, and was considered as Se-conjugated polysaccharides (Se-PFPs).
27865619	10	72	theme	carbohydrate	1065:1076	arg1	w/w					1085:1087	w/w	1085:1087	w/w	1085:1087	This extract contained 93.7% (w/w) of carbohydrate, 2.1% (w/w) of uronic acid and 3.7μg/g of Se, and was considered as Se-conjugated polysaccharides (Se-PFPs).
27865619	6	73	theme	polysaccharides	694:708	arg1	effect					670:675	the anticancer effect	655:675	the anticancer effect of Se-containing polysaccharides from P. fortuneana and the underlying mechanisms	655:757	This study aimed to assess the anticancer effect of Se-containing polysaccharides from P. fortuneana and the underlying mechanisms.
27865619	2	74	theme	Current	250:256	arg1	therapies					258:266	Current therapies	250:266	Current therapies for breast cancer	250:284	Current therapies for breast cancer have adverse side-effects.
27865619	8	75	theme	monosaccharide	826:839	arg1	compositions					841:852	monosaccharide compositions	826:852	monosaccharide compositions	826:852	Their properties and monosaccharide compositions were analyzed.
27865619	0	76	theme	cells	59:63	arg1	growth					18:23	the growth	14:23	the growth of human MDA-MB-231 breast cancer cells	14:63	Inhibition on the growth of human MDA-MB-231 breast cancer cells in vitro and tumor growth in a mouse xenograft model by Se-containing polysaccharides from Pyracantha fortuneana.
27865619	5	77	theme	anticancer	545:554	arg1	activity					556:563	anticancer activity	545:563	anticancer activity on breast cancer	545:580	We hypothesized that Se-containing polysaccharides from P. fortuneana possess anticancer activity on breast cancer via inhibiting growth and inducing apoptosis.
27865619	6	78	theme	anticancer	659:668	arg1	effect					670:675	the anticancer effect	655:675	the anticancer effect of Se-containing polysaccharides from P. fortuneana and the underlying mechanisms	655:757	This study aimed to assess the anticancer effect of Se-containing polysaccharides from P. fortuneana and the underlying mechanisms.
27865619	12	79	theme	MDA-MB-231-derived	1669:1686	arg1	tumors					1698:1703	MDA-MB-231-derived xenograft tumors	1669:1703	MDA-MB-231-derived xenograft tumors	1669:1703	Treatment of nude mice bearing MDA-MB-231-derived xenograft tumors with Se-PFPs significantly reduced tumor growth without altering body weight, confirming its antitumor activity without toxic side effects.
27865619	5	80	contain	possess	537:543	arg1	polysaccharides					502:516	Se-containing polysaccharides	488:516	Se-containing polysaccharides from P. fortuneana	488:535	We hypothesized that Se-containing polysaccharides from P. fortuneana possess anticancer activity on breast cancer via inhibiting growth and inducing apoptosis.
27865619	5	80	contain	possess	537:543	arg2	activity					556:563	anticancer activity	545:563	anticancer activity on breast cancer	545:580	We hypothesized that Se-containing polysaccharides from P. fortuneana possess anticancer activity on breast cancer via inhibiting growth and inducing apoptosis.
27865619	0	81	theme	tumor	78:82	arg1	growth					84:89	tumor growth	78:89	tumor growth	78:89	Inhibition on the growth of human MDA-MB-231 breast cancer cells in vitro and tumor growth in a mouse xenograft model by Se-containing polysaccharides from Pyracantha fortuneana.
27865619	10	82	theme	uronic	1093:1098	arg1	acid					1100:1103	uronic acid	1093:1103	uronic acid	1093:1103	This extract contained 93.7% (w/w) of carbohydrate, 2.1% (w/w) of uronic acid and 3.7μg/g of Se, and was considered as Se-conjugated polysaccharides (Se-PFPs).
27865619	11	83	theme	vitro	1190:1194	arg1	studies					1196:1202	vitro studies	1190:1202	vitro studies	1190:1202	In vitro studies showed that treatment of triple negative breast cancer (TNBC) MDA-MB-231 cells with Se-PFPs (1) inhibited cell growth dose-dependently by arresting cells at G2 phase via inhibiting CDC25C-CyclinB1/CDC2 pathway; (2) caused apoptosis associated with increased p53, Bax, Puma and Noxa, decreased Bcl2, increased Bax/Bcl2 ratio and increased activities of caspases 3/9, suggesting its effect on p53-mediated cytochrome c-caspase pathway.
27865619	3	84	theme	multiple	359:366	arg1	benefits					375:382	multiple health benefits	359:382	multiple health benefits	359:382	Selenium (Se)-containing polysaccharides have multiple health benefits to humans.
27865619	9	85	theme	cycle	913:917	arg1	proteins					919:926	cycle proteins	913:926	cycle proteins	913:926	Their effects on cell growth, expression of cycle proteins, apoptosis and apoptosis-related protein, and tumor growth in mouse xenograft model were examined.
27865619	6	86	theme	underlying	737:746	arg1	mechanisms					748:757	the underlying mechanisms	733:757	the underlying mechanisms	733:757	This study aimed to assess the anticancer effect of Se-containing polysaccharides from P. fortuneana and the underlying mechanisms.
27865619	4	87	contain	contains	433:440	arg2	polysaccharides					450:464	rich Se polysaccharides	442:464	rich Se polysaccharides	442:464	Pyracantha fortuneana (P. fortuneana) contains rich Se polysaccharides.
27865619	4	87	contain	contains	433:440	arg1	fortuneana					406:415	Pyracantha fortuneana	395:415	Pyracantha fortuneana (P. fortuneana)	395:431	Pyracantha fortuneana (P. fortuneana) contains rich Se polysaccharides.
27865619	14	88	theme	potential	2037:2045	arg1	agent					2058:2062	potential anticancer agent	2037:2062	potential anticancer agent for TNBC	2037:2071	It is concluded that Se-containing polysaccharides from P. fortuneana potently inhibit the growth and induce apoptosis of TNBC cells and can be potential anticancer agent for TNBC.
27865619	12	89	theme	mice	1656:1659	arg1	Treatment					1638:1646	Treatment	1638:1646	Treatment of nude mice bearing MDA-MB-231-derived xenograft tumors with Se-PFPs	1638:1716	Treatment of nude mice bearing MDA-MB-231-derived xenograft tumors with Se-PFPs significantly reduced tumor growth without altering body weight, confirming its antitumor activity without toxic side effects.
27865619	0	90	from	fortuneana	167:176	arg1	polysaccharides					135:149	Se-containing polysaccharides	121:149	Se-containing polysaccharides from Pyracantha fortuneana	121:176	Inhibition on the growth of human MDA-MB-231 breast cancer cells in vitro and tumor growth in a mouse xenograft model by Se-containing polysaccharides from Pyracantha fortuneana.
27865619	11	91	theme	cancer	1252:1257	arg1	cells					1277:1281	triple negative breast cancer (TNBC) MDA-MB-231 cells	1229:1281	triple negative breast cancer (TNBC) MDA-MB-231 cells	1229:1281	In vitro studies showed that treatment of triple negative breast cancer (TNBC) MDA-MB-231 cells with Se-PFPs (1) inhibited cell growth dose-dependently by arresting cells at G2 phase via inhibiting CDC25C-CyclinB1/CDC2 pathway; (2) caused apoptosis associated with increased p53, Bax, Puma and Noxa, decreased Bcl2, increased Bax/Bcl2 ratio and increased activities of caspases 3/9, suggesting its effect on p53-mediated cytochrome c-caspase pathway.
27865619	11	92	theme	increased	1452:1460	arg1	p53					1462:1464	increased p53	1452:1464	increased p53	1452:1464	In vitro studies showed that treatment of triple negative breast cancer (TNBC) MDA-MB-231 cells with Se-PFPs (1) inhibited cell growth dose-dependently by arresting cells at G2 phase via inhibiting CDC25C-CyclinB1/CDC2 pathway; (2) caused apoptosis associated with increased p53, Bax, Puma and Noxa, decreased Bcl2, increased Bax/Bcl2 ratio and increased activities of caspases 3/9, suggesting its effect on p53-mediated cytochrome c-caspase pathway.
27865619	0	93	theme	human	28:32	arg1	cells					59:63	human MDA-MB-231 breast cancer cells	28:63	human MDA-MB-231 breast cancer cells	28:63	Inhibition on the growth of human MDA-MB-231 breast cancer cells in vitro and tumor growth in a mouse xenograft model by Se-containing polysaccharides from Pyracantha fortuneana.
27865619	14	94	theme	cells	2020:2024	arg1	apoptosis					2002:2010	apoptosis	2002:2010	apoptosis of TNBC cells	2002:2024	It is concluded that Se-containing polysaccharides from P. fortuneana potently inhibit the growth and induce apoptosis of TNBC cells and can be potential anticancer agent for TNBC.
27865619	9	95	theme	protein	961:967	arg1	expression					899:908	expression	899:908	expression of cycle proteins, apoptosis and apoptosis-related protein	899:967	Their effects on cell growth, expression of cycle proteins, apoptosis and apoptosis-related protein, and tumor growth in mouse xenograft model were examined.
27865619	9	95	theme	protein	961:967	arg1	growth					891:896	cell growth	886:896	cell growth	886:896	Their effects on cell growth, expression of cycle proteins, apoptosis and apoptosis-related protein, and tumor growth in mouse xenograft model were examined.
27865619	9	95	theme	protein	961:967	arg1	growth					980:985	tumor growth	974:985	tumor growth in mouse xenograft model	974:1010	Their effects on cell growth, expression of cycle proteins, apoptosis and apoptosis-related protein, and tumor growth in mouse xenograft model were examined.
27865619	0	96	theme	breast	45:50	arg1	cells					59:63	human MDA-MB-231 breast cancer cells	28:63	human MDA-MB-231 breast cancer cells	28:63	Inhibition on the growth of human MDA-MB-231 breast cancer cells in vitro and tumor growth in a mouse xenograft model by Se-containing polysaccharides from Pyracantha fortuneana.
27865619	12	97	theme	side	1831:1834	arg1	effects					1836:1842	toxic side effects	1825:1842	toxic side effects	1825:1842	Treatment of nude mice bearing MDA-MB-231-derived xenograft tumors with Se-PFPs significantly reduced tumor growth without altering body weight, confirming its antitumor activity without toxic side effects.
27865619	11	98	theme	MDA-MB-231	1266:1275	arg1	cells					1277:1281	triple negative breast cancer (TNBC) MDA-MB-231 cells	1229:1281	triple negative breast cancer (TNBC) MDA-MB-231 cells	1229:1281	In vitro studies showed that treatment of triple negative breast cancer (TNBC) MDA-MB-231 cells with Se-PFPs (1) inhibited cell growth dose-dependently by arresting cells at G2 phase via inhibiting CDC25C-CyclinB1/CDC2 pathway; (2) caused apoptosis associated with increased p53, Bax, Puma and Noxa, decreased Bcl2, increased Bax/Bcl2 ratio and increased activities of caspases 3/9, suggesting its effect on p53-mediated cytochrome c-caspase pathway.
27865619	11	99	theme	cell	1310:1313	arg1	growth					1315:1320	cell growth	1310:1320	cell growth	1310:1320	In vitro studies showed that treatment of triple negative breast cancer (TNBC) MDA-MB-231 cells with Se-PFPs (1) inhibited cell growth dose-dependently by arresting cells at G2 phase via inhibiting CDC25C-CyclinB1/CDC2 pathway; (2) caused apoptosis associated with increased p53, Bax, Puma and Noxa, decreased Bcl2, increased Bax/Bcl2 ratio and increased activities of caspases 3/9, suggesting its effect on p53-mediated cytochrome c-caspase pathway.
27865619	12	100	with	Treatment	1638:1646	arg1	Se-PFPs					1710:1716	Se-PFPs	1710:1716	Se-PFPs	1710:1716	Treatment of nude mice bearing MDA-MB-231-derived xenograft tumors with Se-PFPs significantly reduced tumor growth without altering body weight, confirming its antitumor activity without toxic side effects.
27865619	3	101	contain	have	354:357	arg1	polysaccharides					338:352	Selenium (Se)-containing polysaccharides	313:352	Selenium (Se)-containing polysaccharides	313:352	Selenium (Se)-containing polysaccharides have multiple health benefits to humans.
27865619	3	101	contain	have	354:357	arg2	benefits					375:382	multiple health benefits	359:382	multiple health benefits	359:382	Selenium (Se)-containing polysaccharides have multiple health benefits to humans.
27865619	11	102	theme	TNBC	1260:1263	arg1	cells					1277:1281	triple negative breast cancer (TNBC) MDA-MB-231 cells	1229:1281	triple negative breast cancer (TNBC) MDA-MB-231 cells	1229:1281	In vitro studies showed that treatment of triple negative breast cancer (TNBC) MDA-MB-231 cells with Se-PFPs (1) inhibited cell growth dose-dependently by arresting cells at G2 phase via inhibiting CDC25C-CyclinB1/CDC2 pathway; (2) caused apoptosis associated with increased p53, Bax, Puma and Noxa, decreased Bcl2, increased Bax/Bcl2 ratio and increased activities of caspases 3/9, suggesting its effect on p53-mediated cytochrome c-caspase pathway.
27865619	11	103	theme	p53-mediated	1595:1606	arg1	pathway					1629:1635	p53-mediated cytochrome c-caspase pathway	1595:1635	p53-mediated cytochrome c-caspase pathway	1595:1635	In vitro studies showed that treatment of triple negative breast cancer (TNBC) MDA-MB-231 cells with Se-PFPs (1) inhibited cell growth dose-dependently by arresting cells at G2 phase via inhibiting CDC25C-CyclinB1/CDC2 pathway; (2) caused apoptosis associated with increased p53, Bax, Puma and Noxa, decreased Bcl2, increased Bax/Bcl2 ratio and increased activities of caspases 3/9, suggesting its effect on p53-mediated cytochrome c-caspase pathway.
27865619	12	104	theme	antitumor	1798:1806	arg1	activity					1808:1815	its antitumor activity	1794:1815	its antitumor activity	1794:1815	Treatment of nude mice bearing MDA-MB-231-derived xenograft tumors with Se-PFPs significantly reduced tumor growth without altering body weight, confirming its antitumor activity without toxic side effects.
27865619	9	105	theme	mouse	990:994	arg1	model					1006:1010	mouse xenograft model	990:1010	mouse xenograft model	990:1010	Their effects on cell growth, expression of cycle proteins, apoptosis and apoptosis-related protein, and tumor growth in mouse xenograft model were examined.
27865619	1	106	theme	death	231:235	arg1	cause					207:211	the second cause	196:211	the second cause of cancer-related death among Women	196:247	Breast cancer is the second cause of cancer-related death among Women.
27865619	1	106	theme	death	231:235	arg1	cancer					186:191	Breast cancer	179:191	Breast cancer	179:191	Breast cancer is the second cause of cancer-related death among Women.
28135896	3	0	theme	Sildenafil	302:311	arg1	paste					321:325	Sildenafil citrate paste	302:325	Sildenafil citrate paste	302:325	Sildenafil citrate paste was passed through a nozzle spray bottle to obtain smooth foam.
28135896	4	1	theme	dry	460:462	arg1	granules					469:476	dry foam granules	460:476	dry foam granules	460:476	The homogeneous foam was dried in a vacuum oven and sieved to obtain dry foam granules.
28135896	10	2	theme	sildenafil	1144:1153	arg1	citrate					1182:1188	commercial sildenafil citrate tablet, sildenafil citrate	1133:1188	citrate	1182:1188	The sildenafil citrate dry foam tablet had higher dissolution rate in 0.1 N HCl in comparison with commercial sildenafil citrate tablet, sildenafil citrate prepared by direct compression and wet granulation method.
28135896	1	3	theme	drug	150:153	arg1	dissolution					131:141	dissolution	131:141	dissolution of the drug	131:153	Dry foam formulation technology is alternative approach to enhance dissolution of the drug.
28135896	9	4	theme	disintegration	1006:1019	arg1	<5 min					1026:1031	a disintegration time <5 min	1004:1031	a disintegration time <5 min	1004:1031	The hardness of tablets was about 5 kg, friability test <1% with a disintegration time <5 min.
28135896	7	5	theme	sildenafil	720:729	arg1	citrate					731:737	sildenafil citrate	720:737	sildenafil citrate by both differential scanning calorimetry (DSC) and infrared (IR) analysis	720:812	All the tested excipients were compatible with sildenafil citrate by both differential scanning calorimetry (DSC) and infrared (IR) analysis.
28135896	3	6	theme	citrate	313:319	arg1	paste					321:325	Sildenafil citrate paste	302:325	Sildenafil citrate paste	302:325	Sildenafil citrate paste was passed through a nozzle spray bottle to obtain smooth foam.
28135896	11	7	theme	dry	1268:1270	arg1	tablet					1277:1282	Sildenafil citrate dry foam tablet	1249:1282	Sildenafil citrate dry foam tablet with the high-level composition of surfactant, water and diluent	1249:1347	Sildenafil citrate dry foam tablet with the high-level composition of surfactant, water and diluent showed enhanced dissolution rate than that of the lower-level composition of these excipients.
28135896	4	8	theme	vacuum	427:432	arg1	oven					434:437	a vacuum oven	425:437	a vacuum oven	425:437	The homogeneous foam was dried in a vacuum oven and sieved to obtain dry foam granules.
28135896	8	9	theme	diffraction	834:844	arg1	peaks					852:856	no X-ray diffraction (XRD) peaks	825:856	no X-ray diffraction (XRD) peaks representing crystals of sildenafil citrate observed form dry foam formulations	825:936	There are no X-ray diffraction (XRD) peaks representing crystals of sildenafil citrate observed form dry foam formulations.
28135896	9	10	with	5 kg	973:976	arg1	<5 min					1026:1031	a disintegration time <5 min	1004:1031	a disintegration time <5 min	1004:1031	The hardness of tablets was about 5 kg, friability test <1% with a disintegration time <5 min.
28135896	10	11	theme	wet	1225:1227	arg1	method					1241:1246	wet granulation method	1225:1246	wet granulation method	1225:1246	The sildenafil citrate dry foam tablet had higher dissolution rate in 0.1 N HCl in comparison with commercial sildenafil citrate tablet, sildenafil citrate prepared by direct compression and wet granulation method.
28135896	8	12	theme	X-ray	828:832	arg1	XRD					847:849	XRD	847:849	XRD	847:849	There are no X-ray diffraction (XRD) peaks representing crystals of sildenafil citrate observed form dry foam formulations.
28135896	8	12	theme	X-ray	828:832	arg1	diffraction					834:844	X-ray diffraction	828:844	no X-ray diffraction (XRD) peaks representing crystals of sildenafil citrate observed form dry foam formulations	825:936	There are no X-ray diffraction (XRD) peaks representing crystals of sildenafil citrate observed form dry foam formulations.
28135896	3	13	theme	nozzle	348:353	arg1	spray					355:359	a nozzle spray	346:359	a nozzle spray bottle to obtain smooth foam	346:388	Sildenafil citrate paste was passed through a nozzle spray bottle to obtain smooth foam.
28135896	8	14	theme	citrate	894:900	arg1	crystals					871:878	crystals	871:878	crystals of sildenafil citrate observed form dry foam formulations	871:936	There are no X-ray diffraction (XRD) peaks representing crystals of sildenafil citrate observed form dry foam formulations.
28135896	11	15	theme	excipients	1432:1441	arg1	composition					1411:1421	the lower-level composition	1395:1421	the lower-level composition of these excipients	1395:1441	Sildenafil citrate dry foam tablet with the high-level composition of surfactant, water and diluent showed enhanced dissolution rate than that of the lower-level composition of these excipients.
28135896	5	16	dep	sodium	523:528	arg1	stearate					541:548	stearate	541:548	stearate	541:548	The granules were mixed with croscarmellose sodium, magnesium stearate and compressed into tablet.
28135896	10	17	theme	tablet	1163:1168	arg1	citrate					1182:1188	commercial sildenafil citrate tablet, sildenafil citrate	1133:1188	citrate	1182:1188	The sildenafil citrate dry foam tablet had higher dissolution rate in 0.1 N HCl in comparison with commercial sildenafil citrate tablet, sildenafil citrate prepared by direct compression and wet granulation method.
28135896	10	18	theme	higher	1077:1082	arg1	rate					1096:1099	higher dissolution rate	1077:1099	higher dissolution rate in 0.1 N HCl	1077:1112	The sildenafil citrate dry foam tablet had higher dissolution rate in 0.1 N HCl in comparison with commercial sildenafil citrate tablet, sildenafil citrate prepared by direct compression and wet granulation method.
28135896	10	19	theme	granulation	1229:1239	arg1	method					1241:1246	wet granulation method	1225:1246	wet granulation method	1225:1246	The sildenafil citrate dry foam tablet had higher dissolution rate in 0.1 N HCl in comparison with commercial sildenafil citrate tablet, sildenafil citrate prepared by direct compression and wet granulation method.
28135896	1	20	theme	Dry	64:66	arg1	technology					85:94	Dry foam formulation technology	64:94	Dry foam formulation technology	64:94	Dry foam formulation technology is alternative approach to enhance dissolution of the drug.
28135896	11	21	theme	water	1331:1335	arg1	composition					1304:1314	the high-level composition	1289:1314	the high-level composition of surfactant, water and diluent	1289:1347	Sildenafil citrate dry foam tablet with the high-level composition of surfactant, water and diluent showed enhanced dissolution rate than that of the lower-level composition of these excipients.
28135896	9	22	theme	friability	979:988	arg1	%					997:997	friability test <1%	979:997	friability test <1%	979:997	The hardness of tablets was about 5 kg, friability test <1% with a disintegration time <5 min.
28135896	9	22	theme	friability	979:988	arg1	5 kg					973:976	5 kg	973:976	5 kg	973:976	The hardness of tablets was about 5 kg, friability test <1% with a disintegration time <5 min.
28135896	10	23	theme	foam	1061:1064	arg1	tablet					1066:1071	dry foam tablet	1057:1071	The sildenafil citrate dry foam tablet	1034:1071	The sildenafil citrate dry foam tablet had higher dissolution rate in 0.1 N HCl in comparison with commercial sildenafil citrate tablet, sildenafil citrate prepared by direct compression and wet granulation method.
28135896	1	24	theme	foam	68:71	arg1	technology					85:94	Dry foam formulation technology	64:94	Dry foam formulation technology	64:94	Dry foam formulation technology is alternative approach to enhance dissolution of the drug.
28135896	10	25	from	rate	1096:1099	arg1	HCl					1110:1112	0.1 N HCl	1104:1112	0.1 N HCl	1104:1112	The sildenafil citrate dry foam tablet had higher dissolution rate in 0.1 N HCl in comparison with commercial sildenafil citrate tablet, sildenafil citrate prepared by direct compression and wet granulation method.
28135896	12	26	theme	accelerated	1478:1488	arg1	conditions					1490:1499	accelerated conditions	1478:1499	accelerated conditions	1478:1499	This formulation was stable under accelerated conditions for at least 6 months.
28135896	10	27	theme	dry	1057:1059	arg1	tablet					1066:1071	dry foam tablet	1057:1071	The sildenafil citrate dry foam tablet	1034:1071	The sildenafil citrate dry foam tablet had higher dissolution rate in 0.1 N HCl in comparison with commercial sildenafil citrate tablet, sildenafil citrate prepared by direct compression and wet granulation method.
28135896	11	28	theme	foam	1272:1275	arg1	tablet					1277:1282	Sildenafil citrate dry foam tablet	1249:1282	Sildenafil citrate dry foam tablet with the high-level composition of surfactant, water and diluent	1249:1347	Sildenafil citrate dry foam tablet with the high-level composition of surfactant, water and diluent showed enhanced dissolution rate than that of the lower-level composition of these excipients.
28135896	1	29	theme	formulation	73:83	arg1	technology					85:94	Dry foam formulation technology	64:94	Dry foam formulation technology	64:94	Dry foam formulation technology is alternative approach to enhance dissolution of the drug.
28135896	0	30	theme	Enhanced	0:7	arg1	dissolution					9:19	Enhanced dissolution	0:19	Enhanced dissolution of sildenafil	0:33	Enhanced dissolution of sildenafil citrate as dry foam tablets.
28135896	7	31	theme	tested	681:686	arg1	compatible					704:713	compatible	704:713	compatible	704:713	All the tested excipients were compatible with sildenafil citrate by both differential scanning calorimetry (DSC) and infrared (IR) analysis.
28135896	7	31	theme	tested	681:686	arg1	excipients					688:697	All the tested excipients	673:697	All the tested excipients	673:697	All the tested excipients were compatible with sildenafil citrate by both differential scanning calorimetry (DSC) and infrared (IR) analysis.
28135896	11	32	theme	surfactant	1319:1328	arg1	composition					1304:1314	the high-level composition	1289:1314	the high-level composition of surfactant, water and diluent	1289:1347	Sildenafil citrate dry foam tablet with the high-level composition of surfactant, water and diluent showed enhanced dissolution rate than that of the lower-level composition of these excipients.
28135896	11	33	theme	Sildenafil	1249:1258	arg1	citrate					1260:1266	Sildenafil citrate	1249:1266	Sildenafil citrate dry foam tablet with the high-level composition of surfactant, water and diluent	1249:1347	Sildenafil citrate dry foam tablet with the high-level composition of surfactant, water and diluent showed enhanced dissolution rate than that of the lower-level composition of these excipients.
28135896	0	34	theme	sildenafil	24:33	arg1	dissolution					9:19	Enhanced dissolution	0:19	Enhanced dissolution of sildenafil	0:33	Enhanced dissolution of sildenafil citrate as dry foam tablets.
28135896	11	35	theme	high-level	1293:1302	arg1	composition					1304:1314	the high-level composition	1289:1314	the high-level composition of surfactant, water and diluent	1289:1347	Sildenafil citrate dry foam tablet with the high-level composition of surfactant, water and diluent showed enhanced dissolution rate than that of the lower-level composition of these excipients.
28135896	3	36	theme	bottle	361:366	arg1	spray					355:359	a nozzle spray	346:359	a nozzle spray bottle to obtain smooth foam	346:388	Sildenafil citrate paste was passed through a nozzle spray bottle to obtain smooth foam.
28135896	10	37	theme	dissolution	1084:1094	arg1	rate					1096:1099	higher dissolution rate	1077:1099	higher dissolution rate in 0.1 N HCl	1077:1112	The sildenafil citrate dry foam tablet had higher dissolution rate in 0.1 N HCl in comparison with commercial sildenafil citrate tablet, sildenafil citrate prepared by direct compression and wet granulation method.
28135896	1	38	theme	alternative	99:109	arg1	approach					111:118	alternative approach	99:118	alternative approach to enhance dissolution of the drug	99:153	Dry foam formulation technology is alternative approach to enhance dissolution of the drug.
28135896	8	39	theme	sildenafil	883:892	arg1	citrate					894:900	sildenafil citrate	883:900	sildenafil citrate observed form dry foam formulations	883:936	There are no X-ray diffraction (XRD) peaks representing crystals of sildenafil citrate observed form dry foam formulations.
28135896	7	40	theme	infrared	791:798	arg1	analysis					805:812	infrared (IR) analysis	791:812	infrared (IR) analysis	791:812	All the tested excipients were compatible with sildenafil citrate by both differential scanning calorimetry (DSC) and infrared (IR) analysis.
28135896	7	40	theme	infrared	791:798	arg1	IR					801:802	IR	801:802	IR	801:802	All the tested excipients were compatible with sildenafil citrate by both differential scanning calorimetry (DSC) and infrared (IR) analysis.
28135896	9	41	theme	time	1021:1024	arg1	<5 min					1026:1031	a disintegration time <5 min	1004:1031	a disintegration time <5 min	1004:1031	The hardness of tablets was about 5 kg, friability test <1% with a disintegration time <5 min.
28135896	6	42	theme	dissolution	651:661	arg1	profiles					663:670	dissolution profiles	651:670	dissolution profiles	651:670	All formulations were evaluated for their physicochemical properties and dissolution profiles.
28135896	11	43	theme	diluent	1341:1347	arg1	composition					1304:1314	the high-level composition	1289:1314	the high-level composition of surfactant, water and diluent	1289:1347	Sildenafil citrate dry foam tablet with the high-level composition of surfactant, water and diluent showed enhanced dissolution rate than that of the lower-level composition of these excipients.
28135896	10	44	dep	citrate	1182:1188	arg1	sildenafil					1171:1180	commercial sildenafil citrate tablet, sildenafil citrate	1133:1188	sildenafil	1171:1180	The sildenafil citrate dry foam tablet had higher dissolution rate in 0.1 N HCl in comparison with commercial sildenafil citrate tablet, sildenafil citrate prepared by direct compression and wet granulation method.
28135896	9	45	theme	tablets	955:961	arg1	5 kg					973:976	5 kg	973:976	5 kg	973:976	The hardness of tablets was about 5 kg, friability test <1% with a disintegration time <5 min.
28135896	9	45	theme	tablets	955:961	arg1	hardness					943:950	The hardness	939:950	The hardness of tablets	939:961	The hardness of tablets was about 5 kg, friability test <1% with a disintegration time <5 min.
28135896	7	46	with	compatible	704:713	arg1	citrate					731:737	sildenafil citrate	720:737	sildenafil citrate by both differential scanning calorimetry (DSC) and infrared (IR) analysis	720:812	All the tested excipients were compatible with sildenafil citrate by both differential scanning calorimetry (DSC) and infrared (IR) analysis.
28135896	0	47	theme	foam	50:53	arg1	tablets					55:61	dry foam tablets	46:61	dry foam tablets	46:61	Enhanced dissolution of sildenafil citrate as dry foam tablets.
28135896	2	48	theme	Sildenafil	156:165	arg1	citrate					167:173	Sildenafil citrate	156:173	Sildenafil citrate	156:173	Sildenafil citrate was suspended in sodium dodecyl sulfate solution and adding a mixture of maltodextrin and mannitol as diluent to form a paste.
28135896	5	49	theme	croscarmellose	508:521	arg1	magnesium					531:539	magnesium	531:539	magnesium	531:539	The granules were mixed with croscarmellose sodium, magnesium stearate and compressed into tablet.
28135896	5	49	theme	croscarmellose	508:521	arg1	sodium					523:528	croscarmellose sodium	508:528	croscarmellose sodium	508:528	The granules were mixed with croscarmellose sodium, magnesium stearate and compressed into tablet.
28135896	0	50	theme	dry	46:48	arg1	tablets					55:61	dry foam tablets	46:61	dry foam tablets	46:61	Enhanced dissolution of sildenafil citrate as dry foam tablets.
28135896	10	51	dep	citrate	1049:1055	arg1	tablet					1066:1071	dry foam tablet	1057:1071	The sildenafil citrate dry foam tablet	1034:1071	The sildenafil citrate dry foam tablet had higher dissolution rate in 0.1 N HCl in comparison with commercial sildenafil citrate tablet, sildenafil citrate prepared by direct compression and wet granulation method.
28135896	2	52	theme	sulfate	207:213	arg1	solution					215:222	sodium dodecyl sulfate solution	192:222	sodium dodecyl sulfate solution	192:222	Sildenafil citrate was suspended in sodium dodecyl sulfate solution and adding a mixture of maltodextrin and mannitol as diluent to form a paste.
28135896	4	53	theme	homogeneous	395:405	arg1	foam					407:410	The homogeneous foam	391:410	The homogeneous foam	391:410	The homogeneous foam was dried in a vacuum oven and sieved to obtain dry foam granules.
28135896	8	54	theme	dry	916:918	arg1	formulations					925:936	dry foam formulations	916:936	dry foam formulations	916:936	There are no X-ray diffraction (XRD) peaks representing crystals of sildenafil citrate observed form dry foam formulations.
28135896	11	55	with	tablet	1277:1282	arg1	composition					1304:1314	the high-level composition	1289:1314	the high-level composition of surfactant, water and diluent	1289:1347	Sildenafil citrate dry foam tablet with the high-level composition of surfactant, water and diluent showed enhanced dissolution rate than that of the lower-level composition of these excipients.
28135896	9	56	theme	test	990:993	arg1	%					997:997	friability test <1%	979:997	friability test <1%	979:997	The hardness of tablets was about 5 kg, friability test <1% with a disintegration time <5 min.
28135896	9	56	theme	test	990:993	arg1	5 kg					973:976	5 kg	973:976	5 kg	973:976	The hardness of tablets was about 5 kg, friability test <1% with a disintegration time <5 min.
28135896	10	57	theme	0.1 N	1104:1108	arg1	HCl					1110:1112	0.1 N HCl	1104:1112	0.1 N HCl	1104:1112	The sildenafil citrate dry foam tablet had higher dissolution rate in 0.1 N HCl in comparison with commercial sildenafil citrate tablet, sildenafil citrate prepared by direct compression and wet granulation method.
28135896	2	58	theme	dodecyl	199:205	arg1	sulfate					207:213	sodium dodecyl sulfate	192:213	sodium dodecyl sulfate solution	192:222	Sildenafil citrate was suspended in sodium dodecyl sulfate solution and adding a mixture of maltodextrin and mannitol as diluent to form a paste.
28135896	10	59	contain	had	1073:1075	arg1	citrate					1049:1055	The sildenafil citrate dry foam tablet	1034:1071	The sildenafil citrate dry foam tablet	1034:1071	The sildenafil citrate dry foam tablet had higher dissolution rate in 0.1 N HCl in comparison with commercial sildenafil citrate tablet, sildenafil citrate prepared by direct compression and wet granulation method.
28135896	10	59	contain	had	1073:1075	arg2	rate					1096:1099	higher dissolution rate	1077:1099	higher dissolution rate in 0.1 N HCl	1077:1112	The sildenafil citrate dry foam tablet had higher dissolution rate in 0.1 N HCl in comparison with commercial sildenafil citrate tablet, sildenafil citrate prepared by direct compression and wet granulation method.
28135896	11	60	theme	lower-level	1399:1409	arg1	composition					1411:1421	the lower-level composition	1395:1421	the lower-level composition of these excipients	1395:1441	Sildenafil citrate dry foam tablet with the high-level composition of surfactant, water and diluent showed enhanced dissolution rate than that of the lower-level composition of these excipients.
28135896	11	61	theme	enhanced	1356:1363	arg1	rate					1377:1380	enhanced dissolution rate	1356:1380	enhanced dissolution rate than that of the lower-level composition of these excipients	1356:1441	Sildenafil citrate dry foam tablet with the high-level composition of surfactant, water and diluent showed enhanced dissolution rate than that of the lower-level composition of these excipients.
28135896	10	62	theme	direct	1202:1207	arg1	compression					1209:1219	direct compression	1202:1219	direct compression	1202:1219	The sildenafil citrate dry foam tablet had higher dissolution rate in 0.1 N HCl in comparison with commercial sildenafil citrate tablet, sildenafil citrate prepared by direct compression and wet granulation method.
28135896	7	63	theme	differential	747:758	arg1	DSC					782:784	DSC	782:784	DSC	782:784	All the tested excipients were compatible with sildenafil citrate by both differential scanning calorimetry (DSC) and infrared (IR) analysis.
28135896	7	63	theme	differential	747:758	arg1	calorimetry					769:779	both differential scanning calorimetry	742:779	both differential scanning calorimetry (DSC)	742:785	All the tested excipients were compatible with sildenafil citrate by both differential scanning calorimetry (DSC) and infrared (IR) analysis.
28135896	2	64	theme	mannitol	265:272	arg1	mixture					237:243	a mixture	235:243	a mixture of maltodextrin and mannitol	235:272	Sildenafil citrate was suspended in sodium dodecyl sulfate solution and adding a mixture of maltodextrin and mannitol as diluent to form a paste.
28135896	2	65	theme	sodium	192:197	arg1	sulfate					207:213	sodium dodecyl sulfate	192:213	sodium dodecyl sulfate solution	192:222	Sildenafil citrate was suspended in sodium dodecyl sulfate solution and adding a mixture of maltodextrin and mannitol as diluent to form a paste.
28135896	2	66	theme	maltodextrin	248:259	arg1	mixture					237:243	a mixture	235:243	a mixture of maltodextrin and mannitol	235:272	Sildenafil citrate was suspended in sodium dodecyl sulfate solution and adding a mixture of maltodextrin and mannitol as diluent to form a paste.
28135896	10	67	theme	sildenafil	1038:1047	arg1	citrate					1049:1055	The sildenafil citrate dry foam tablet	1034:1071	The sildenafil citrate dry foam tablet	1034:1071	The sildenafil citrate dry foam tablet had higher dissolution rate in 0.1 N HCl in comparison with commercial sildenafil citrate tablet, sildenafil citrate prepared by direct compression and wet granulation method.
28135896	6	68	theme	physicochemical	620:634	arg1	properties					636:645	their physicochemical properties	614:645	their physicochemical properties	614:645	All formulations were evaluated for their physicochemical properties and dissolution profiles.
28135896	7	69	theme	scanning	760:767	arg1	DSC					782:784	DSC	782:784	DSC	782:784	All the tested excipients were compatible with sildenafil citrate by both differential scanning calorimetry (DSC) and infrared (IR) analysis.
28135896	7	69	theme	scanning	760:767	arg1	calorimetry					769:779	both differential scanning calorimetry	742:779	both differential scanning calorimetry (DSC)	742:785	All the tested excipients were compatible with sildenafil citrate by both differential scanning calorimetry (DSC) and infrared (IR) analysis.
28135896	11	70	theme	dissolution	1365:1375	arg1	rate					1377:1380	enhanced dissolution rate	1356:1380	enhanced dissolution rate than that of the lower-level composition of these excipients	1356:1441	Sildenafil citrate dry foam tablet with the high-level composition of surfactant, water and diluent showed enhanced dissolution rate than that of the lower-level composition of these excipients.
28135896	10	71	with	comparison	1117:1126	arg1	citrate					1182:1188	commercial sildenafil citrate tablet, sildenafil citrate	1133:1188	citrate	1182:1188	The sildenafil citrate dry foam tablet had higher dissolution rate in 0.1 N HCl in comparison with commercial sildenafil citrate tablet, sildenafil citrate prepared by direct compression and wet granulation method.
28135896	8	72	theme	foam	920:923	arg1	formulations					925:936	dry foam formulations	916:936	dry foam formulations	916:936	There are no X-ray diffraction (XRD) peaks representing crystals of sildenafil citrate observed form dry foam formulations.
28135896	11	73	theme	citrate	1260:1266	arg1	tablet					1277:1282	Sildenafil citrate dry foam tablet	1249:1282	Sildenafil citrate dry foam tablet with the high-level composition of surfactant, water and diluent	1249:1347	Sildenafil citrate dry foam tablet with the high-level composition of surfactant, water and diluent showed enhanced dissolution rate than that of the lower-level composition of these excipients.
28135896	10	74	theme	commercial	1133:1142	arg1	citrate					1182:1188	commercial sildenafil citrate tablet, sildenafil citrate	1133:1188	citrate	1182:1188	The sildenafil citrate dry foam tablet had higher dissolution rate in 0.1 N HCl in comparison with commercial sildenafil citrate tablet, sildenafil citrate prepared by direct compression and wet granulation method.
28135896	3	75	theme	smooth	378:383	arg1	foam					385:388	smooth foam	378:388	smooth foam	378:388	Sildenafil citrate paste was passed through a nozzle spray bottle to obtain smooth foam.
28135896	4	76	theme	foam	464:467	arg1	granules					469:476	dry foam granules	460:476	dry foam granules	460:476	The homogeneous foam was dried in a vacuum oven and sieved to obtain dry foam granules.
28135896	10	77	theme	citrate	1155:1161	arg1	citrate					1182:1188	commercial sildenafil citrate tablet, sildenafil citrate	1133:1188	citrate	1182:1188	The sildenafil citrate dry foam tablet had higher dissolution rate in 0.1 N HCl in comparison with commercial sildenafil citrate tablet, sildenafil citrate prepared by direct compression and wet granulation method.
27481894	4	0	theme	cell	828:831	arg1	epitope					838:844	Each cell wall epitope	823:844	Each cell wall epitope	823:844	Each cell wall epitope showed a characteristic temporal and spatial labeling pattern.
27481894	9	1	theme	stage-specific	1679:1692	arg1	manner					1694:1699	a tissue- and stage-specific manner	1665:1699	a tissue- and stage-specific manner	1665:1699	The distribution of the different cell wall constituents is regulated in a tissue- and stage-specific manner, which seems directly related to the role of each tissue at each stage.
27481894	5	2	dep	layers	1017:1022	arg1	layers					1017:1022	the outermost anther layers	996:1022	the outermost anther layers (epidermis, endothecium and middle layers)	996:1065	Microspore, pollen and tapetal cells showed similar patterns for each epitope, whereas the outermost anther layers (epidermis, endothecium and middle layers) presented remarkably different patterns.
27481894	5	2	dep	layers	1017:1022	arg1	endothecium					1036:1046	endothecium	1036:1046	endothecium	1036:1046	Microspore, pollen and tapetal cells showed similar patterns for each epitope, whereas the outermost anther layers (epidermis, endothecium and middle layers) presented remarkably different patterns.
27481894	5	2	dep	layers	1017:1022	arg1	layers					1059:1064	middle layers	1052:1064	middle layers	1052:1064	Microspore, pollen and tapetal cells showed similar patterns for each epitope, whereas the outermost anther layers (epidermis, endothecium and middle layers) presented remarkably different patterns.
27481894	5	2	dep	layers	1017:1022	arg1	epidermis					1025:1033	epidermis	1025:1033	epidermis	1025:1033	Microspore, pollen and tapetal cells showed similar patterns for each epitope, whereas the outermost anther layers (epidermis, endothecium and middle layers) presented remarkably different patterns.
27481894	8	3	from	role	1517:1520	arg1	dehiscence					1532:1541	anther dehiscence	1525:1541	anther dehiscence	1525:1541	In contrast, the changes in pectin and hemicellulose epitopes suggested a specific role in anther dehiscence, facilitating anther wall weakening and rupture.
27481894	1	4	theme	napus	261:265	arg1	development					274:284	Brassica napus anther development	252:284	Brassica napus anther development	252:284	In this work, we performed an extensive and detailed analysis of the changes in cell wall composition during Brassica napus anther development.
27481894	9	5	theme	different	1616:1624	arg1	constituents					1636:1647	the different cell wall constituents	1612:1647	the different cell wall constituents	1612:1647	The distribution of the different cell wall constituents is regulated in a tissue- and stage-specific manner, which seems directly related to the role of each tissue at each stage.
27481894	9	6	from	stage	1766:1770	arg1	role					1738:1741	the role	1734:1741	the role of each tissue at each stage	1734:1770	The distribution of the different cell wall constituents is regulated in a tissue- and stage-specific manner, which seems directly related to the role of each tissue at each stage.
27481894	9	7	theme	wall	1631:1634	arg1	constituents					1636:1647	the different cell wall constituents	1612:1647	the different cell wall constituents	1612:1647	The distribution of the different cell wall constituents is regulated in a tissue- and stage-specific manner, which seems directly related to the role of each tissue at each stage.
27481894	2	8	theme	different	583:591	arg1	tissues					600:606	the different anther tissues	579:606	the different anther tissues	579:606	We used immunogold labeling to study the spatial and temporal patterns of the composition and distribution of different arabinogalactan protein (AGP), pectin, xyloglucan and xylan epitopes in high-pressure-frozen/freeze-substituted anthers, quantifying and comparing their relative levels in the different anther tissues and developmental stages.
27481894	2	9	from	composition	365:375	arg1	anthers					519:525	high-pressure-frozen/freeze-substituted anthers	479:525	high-pressure-frozen/freeze-substituted anthers	479:525	We used immunogold labeling to study the spatial and temporal patterns of the composition and distribution of different arabinogalactan protein (AGP), pectin, xyloglucan and xylan epitopes in high-pressure-frozen/freeze-substituted anthers, quantifying and comparing their relative levels in the different anther tissues and developmental stages.
27481894	9	10	theme	tissue-	1667:1673	arg1	manner					1694:1699	a tissue- and stage-specific manner	1665:1699	a tissue- and stage-specific manner	1665:1699	The distribution of the different cell wall constituents is regulated in a tissue- and stage-specific manner, which seems directly related to the role of each tissue at each stage.
27481894	6	11	theme	anther	1198:1203	arg1	development					1205:1215	anther development	1198:1215	anther development	1198:1215	Our results suggested that AGPs, pectins, xyloglucan and xylan have specific roles during anther development.
27481894	8	12	from	changes	1451:1457	arg1	epitopes					1487:1494	pectin and hemicellulose epitopes	1462:1494	pectin and hemicellulose epitopes	1462:1494	In contrast, the changes in pectin and hemicellulose epitopes suggested a specific role in anther dehiscence, facilitating anther wall weakening and rupture.
27481894	1	13	from	changes	212:218	arg1	composition					233:243	cell wall composition	223:243	cell wall composition	223:243	In this work, we performed an extensive and detailed analysis of the changes in cell wall composition during Brassica napus anther development.
27481894	3	14	dep	antibodies	667:676	arg1	LM11					807:810	LM11	807:810	LM11	807:810	We used the following monoclonal antibodies: JIM13, JIM8, JIM14 and JIM16 for AGPs, LM5, LM6, JIM7, JIM5 and LM7 for pectins, CCRC-M1, CCRC-M89 and LM15 for xyloglucan, and LM11 for xylan.
27481894	3	14	dep	antibodies	667:676	arg1	JIM13					679:683	JIM13	679:683	JIM13	679:683	We used the following monoclonal antibodies: JIM13, JIM8, JIM14 and JIM16 for AGPs, LM5, LM6, JIM7, JIM5 and LM7 for pectins, CCRC-M1, CCRC-M89 and LM15 for xyloglucan, and LM11 for xylan.
27481894	3	14	dep	antibodies	667:676	arg1	JIM8					686:689	JIM8	686:689	JIM8	686:689	We used the following monoclonal antibodies: JIM13, JIM8, JIM14 and JIM16 for AGPs, LM5, LM6, JIM7, JIM5 and LM7 for pectins, CCRC-M1, CCRC-M89 and LM15 for xyloglucan, and LM11 for xylan.
27481894	3	14	dep	antibodies	667:676	arg1	CCRC-M1					760:766	CCRC-M1	760:766	CCRC-M1	760:766	We used the following monoclonal antibodies: JIM13, JIM8, JIM14 and JIM16 for AGPs, LM5, LM6, JIM7, JIM5 and LM7 for pectins, CCRC-M1, CCRC-M89 and LM15 for xyloglucan, and LM11 for xylan.
27481894	3	14	dep	antibodies	667:676	arg1	antibodies					667:676	the following monoclonal antibodies	642:676	the following monoclonal antibodies: JIM13, JIM8, JIM14 and JIM16 for AGPs, LM5, LM6, JIM7, JIM5 and LM7 for pectins, CCRC-M1, CCRC-M89 and LM15 for xyloglucan, and LM11 for xylan	642:820	We used the following monoclonal antibodies: JIM13, JIM8, JIM14 and JIM16 for AGPs, LM5, LM6, JIM7, JIM5 and LM7 for pectins, CCRC-M1, CCRC-M89 and LM15 for xyloglucan, and LM11 for xylan.
27481894	3	14	dep	antibodies	667:676	arg1	JIM14					692:696	JIM14	692:696	JIM14	692:696	We used the following monoclonal antibodies: JIM13, JIM8, JIM14 and JIM16 for AGPs, LM5, LM6, JIM7, JIM5 and LM7 for pectins, CCRC-M1, CCRC-M89 and LM15 for xyloglucan, and LM11 for xylan.
27481894	3	14	dep	antibodies	667:676	arg1	CCRC-M89					769:776	CCRC-M89	769:776	CCRC-M89	769:776	We used the following monoclonal antibodies: JIM13, JIM8, JIM14 and JIM16 for AGPs, LM5, LM6, JIM7, JIM5 and LM7 for pectins, CCRC-M1, CCRC-M89 and LM15 for xyloglucan, and LM11 for xylan.
27481894	2	15	theme	developmental	612:624	arg1	stages					626:631	developmental stages	612:631	developmental stages	612:631	We used immunogold labeling to study the spatial and temporal patterns of the composition and distribution of different arabinogalactan protein (AGP), pectin, xyloglucan and xylan epitopes in high-pressure-frozen/freeze-substituted anthers, quantifying and comparing their relative levels in the different anther tissues and developmental stages.
27481894	5	16	theme	outermost	1000:1008	arg1	layers					1017:1022	the outermost anther layers	996:1022	the outermost anther layers (epidermis, endothecium and middle layers)	996:1065	Microspore, pollen and tapetal cells showed similar patterns for each epitope, whereas the outermost anther layers (epidermis, endothecium and middle layers) presented remarkably different patterns.
27481894	5	16	theme	outermost	1000:1008	arg1	endothecium					1036:1046	endothecium	1036:1046	endothecium	1036:1046	Microspore, pollen and tapetal cells showed similar patterns for each epitope, whereas the outermost anther layers (epidermis, endothecium and middle layers) presented remarkably different patterns.
27481894	5	16	theme	outermost	1000:1008	arg1	epidermis					1025:1033	epidermis	1025:1033	epidermis	1025:1033	Microspore, pollen and tapetal cells showed similar patterns for each epitope, whereas the outermost anther layers (epidermis, endothecium and middle layers) presented remarkably different patterns.
27481894	4	17	theme	temporal	870:877	arg1	pattern					900:906	a characteristic temporal and spatial labeling pattern	853:906	a characteristic temporal and spatial labeling pattern	853:906	Each cell wall epitope showed a characteristic temporal and spatial labeling pattern.
27481894	3	18	used	used	637:640	arg2	We					634:635	We	634:635	We	634:635	We used the following monoclonal antibodies: JIM13, JIM8, JIM14 and JIM16 for AGPs, LM5, LM6, JIM7, JIM5 and LM7 for pectins, CCRC-M1, CCRC-M89 and LM15 for xyloglucan, and LM11 for xylan.
27481894	0	19	from	Development	112:122	arg1	Brassica					127:134	Brassica	127:134	Brassica	127:134	Ultrastructural Immunolocalization of Arabinogalactan Protein, Pectin and Hemicellulose Epitopes Through Anther Development in Brassica napus.
27481894	0	20	theme	Hemicellulose	74:86	arg1	Immunolocalization					16:33	Ultrastructural Immunolocalization	0:33	Ultrastructural Immunolocalization of Arabinogalactan Protein, Pectin and Hemicellulose	0:86	Ultrastructural Immunolocalization of Arabinogalactan Protein, Pectin and Hemicellulose Epitopes Through Anther Development in Brassica napus.
27481894	3	21	theme	following	646:654	arg1	LM11					807:810	LM11	807:810	LM11	807:810	We used the following monoclonal antibodies: JIM13, JIM8, JIM14 and JIM16 for AGPs, LM5, LM6, JIM7, JIM5 and LM7 for pectins, CCRC-M1, CCRC-M89 and LM15 for xyloglucan, and LM11 for xylan.
27481894	3	21	theme	following	646:654	arg1	LM15					782:785	LM15	782:785	LM15	782:785	We used the following monoclonal antibodies: JIM13, JIM8, JIM14 and JIM16 for AGPs, LM5, LM6, JIM7, JIM5 and LM7 for pectins, CCRC-M1, CCRC-M89 and LM15 for xyloglucan, and LM11 for xylan.
27481894	3	21	theme	following	646:654	arg1	JIM13					679:683	JIM13	679:683	JIM13	679:683	We used the following monoclonal antibodies: JIM13, JIM8, JIM14 and JIM16 for AGPs, LM5, LM6, JIM7, JIM5 and LM7 for pectins, CCRC-M1, CCRC-M89 and LM15 for xyloglucan, and LM11 for xylan.
27481894	3	21	theme	following	646:654	arg1	JIM8					686:689	JIM8	686:689	JIM8	686:689	We used the following monoclonal antibodies: JIM13, JIM8, JIM14 and JIM16 for AGPs, LM5, LM6, JIM7, JIM5 and LM7 for pectins, CCRC-M1, CCRC-M89 and LM15 for xyloglucan, and LM11 for xylan.
27481894	3	21	theme	following	646:654	arg1	CCRC-M1					760:766	CCRC-M1	760:766	CCRC-M1	760:766	We used the following monoclonal antibodies: JIM13, JIM8, JIM14 and JIM16 for AGPs, LM5, LM6, JIM7, JIM5 and LM7 for pectins, CCRC-M1, CCRC-M89 and LM15 for xyloglucan, and LM11 for xylan.
27481894	3	21	theme	following	646:654	arg1	antibodies					667:676	the following monoclonal antibodies	642:676	the following monoclonal antibodies: JIM13, JIM8, JIM14 and JIM16 for AGPs, LM5, LM6, JIM7, JIM5 and LM7 for pectins, CCRC-M1, CCRC-M89 and LM15 for xyloglucan, and LM11 for xylan	642:820	We used the following monoclonal antibodies: JIM13, JIM8, JIM14 and JIM16 for AGPs, LM5, LM6, JIM7, JIM5 and LM7 for pectins, CCRC-M1, CCRC-M89 and LM15 for xyloglucan, and LM11 for xylan.
27481894	3	21	theme	following	646:654	arg1	JIM14					692:696	JIM14	692:696	JIM14	692:696	We used the following monoclonal antibodies: JIM13, JIM8, JIM14 and JIM16 for AGPs, LM5, LM6, JIM7, JIM5 and LM7 for pectins, CCRC-M1, CCRC-M89 and LM15 for xyloglucan, and LM11 for xylan.
27481894	3	21	theme	following	646:654	arg1	CCRC-M89					769:776	CCRC-M89	769:776	CCRC-M89	769:776	We used the following monoclonal antibodies: JIM13, JIM8, JIM14 and JIM16 for AGPs, LM5, LM6, JIM7, JIM5 and LM7 for pectins, CCRC-M1, CCRC-M89 and LM15 for xyloglucan, and LM11 for xylan.
27481894	3	21	theme	following	646:654	arg1	JIM16					702:706	JIM16	702:706	JIM16	702:706	We used the following monoclonal antibodies: JIM13, JIM8, JIM14 and JIM16 for AGPs, LM5, LM6, JIM7, JIM5 and LM7 for pectins, CCRC-M1, CCRC-M89 and LM15 for xyloglucan, and LM11 for xylan.
27481894	2	22	theme	anther	593:598	arg1	tissues					600:606	the different anther tissues	579:606	the different anther tissues	579:606	We used immunogold labeling to study the spatial and temporal patterns of the composition and distribution of different arabinogalactan protein (AGP), pectin, xyloglucan and xylan epitopes in high-pressure-frozen/freeze-substituted anthers, quantifying and comparing their relative levels in the different anther tissues and developmental stages.
27481894	4	23	theme	wall	833:836	arg1	epitope					838:844	Each cell wall epitope	823:844	Each cell wall epitope	823:844	Each cell wall epitope showed a characteristic temporal and spatial labeling pattern.
27481894	2	24	theme	relative	560:567	arg1	levels					569:574	their relative levels	554:574	their relative levels in the different anther tissues and developmental stages	554:631	We used immunogold labeling to study the spatial and temporal patterns of the composition and distribution of different arabinogalactan protein (AGP), pectin, xyloglucan and xylan epitopes in high-pressure-frozen/freeze-substituted anthers, quantifying and comparing their relative levels in the different anther tissues and developmental stages.
27481894	5	25	theme	middle	1052:1057	arg1	layers					1059:1064	middle layers	1052:1064	middle layers	1052:1064	Microspore, pollen and tapetal cells showed similar patterns for each epitope, whereas the outermost anther layers (epidermis, endothecium and middle layers) presented remarkably different patterns.
27481894	0	26	dep	Brassica	127:134	arg1	napus					136:140	Brassica napus	127:140	Brassica napus	127:140	Ultrastructural Immunolocalization of Arabinogalactan Protein, Pectin and Hemicellulose Epitopes Through Anther Development in Brassica napus.
27481894	2	27	theme	arabinogalactan	407:421	arg1	AGP					432:434	AGP	432:434	AGP	432:434	We used immunogold labeling to study the spatial and temporal patterns of the composition and distribution of different arabinogalactan protein (AGP), pectin, xyloglucan and xylan epitopes in high-pressure-frozen/freeze-substituted anthers, quantifying and comparing their relative levels in the different anther tissues and developmental stages.
27481894	2	27	theme	arabinogalactan	407:421	arg1	protein					423:429	different arabinogalactan protein	397:429	different arabinogalactan protein (AGP)	397:435	We used immunogold labeling to study the spatial and temporal patterns of the composition and distribution of different arabinogalactan protein (AGP), pectin, xyloglucan and xylan epitopes in high-pressure-frozen/freeze-substituted anthers, quantifying and comparing their relative levels in the different anther tissues and developmental stages.
27481894	2	28	theme	xyloglucan	446:455	arg1	epitopes					467:474	different arabinogalactan protein (AGP), pectin, xyloglucan and xylan epitopes	397:474	different arabinogalactan protein (AGP), pectin, xyloglucan and xylan epitopes in high-pressure-frozen/freeze-substituted anthers	397:525	We used immunogold labeling to study the spatial and temporal patterns of the composition and distribution of different arabinogalactan protein (AGP), pectin, xyloglucan and xylan epitopes in high-pressure-frozen/freeze-substituted anthers, quantifying and comparing their relative levels in the different anther tissues and developmental stages.
27481894	2	29	from	epitopes	467:474	arg1	anthers					519:525	high-pressure-frozen/freeze-substituted anthers	479:525	high-pressure-frozen/freeze-substituted anthers	479:525	We used immunogold labeling to study the spatial and temporal patterns of the composition and distribution of different arabinogalactan protein (AGP), pectin, xyloglucan and xylan epitopes in high-pressure-frozen/freeze-substituted anthers, quantifying and comparing their relative levels in the different anther tissues and developmental stages.
27481894	0	30	theme	Ultrastructural	0:14	arg1	Immunolocalization					16:33	Ultrastructural Immunolocalization	0:33	Ultrastructural Immunolocalization of Arabinogalactan Protein, Pectin and Hemicellulose	0:86	Ultrastructural Immunolocalization of Arabinogalactan Protein, Pectin and Hemicellulose Epitopes Through Anther Development in Brassica napus.
27481894	5	31	theme	Microspore	909:918	arg1	cells					940:944	Microspore, pollen and tapetal cells	909:944	Microspore, pollen and tapetal cells	909:944	Microspore, pollen and tapetal cells showed similar patterns for each epitope, whereas the outermost anther layers (epidermis, endothecium and middle layers) presented remarkably different patterns.
27481894	2	32	theme	pectin	438:443	arg1	epitopes					467:474	different arabinogalactan protein (AGP), pectin, xyloglucan and xylan epitopes	397:474	different arabinogalactan protein (AGP), pectin, xyloglucan and xylan epitopes in high-pressure-frozen/freeze-substituted anthers	397:525	We used immunogold labeling to study the spatial and temporal patterns of the composition and distribution of different arabinogalactan protein (AGP), pectin, xyloglucan and xylan epitopes in high-pressure-frozen/freeze-substituted anthers, quantifying and comparing their relative levels in the different anther tissues and developmental stages.
27481894	5	33	theme	pollen	921:926	arg1	cells					940:944	Microspore, pollen and tapetal cells	909:944	Microspore, pollen and tapetal cells	909:944	Microspore, pollen and tapetal cells showed similar patterns for each epitope, whereas the outermost anther layers (epidermis, endothecium and middle layers) presented remarkably different patterns.
27481894	0	34	theme	Protein	54:60	arg1	Immunolocalization					16:33	Ultrastructural Immunolocalization	0:33	Ultrastructural Immunolocalization of Arabinogalactan Protein, Pectin and Hemicellulose	0:86	Ultrastructural Immunolocalization of Arabinogalactan Protein, Pectin and Hemicellulose Epitopes Through Anther Development in Brassica napus.
27481894	7	35	dep	endothecium	1403:1413	arg1	the					1399:1401	the	1399:1401	the	1399:1401	The AGP epitopes studied appeared to belong to AGPs specifically involved in microspore differentiation, and contributed first by the tapetum and then, upon tapetal dismantling, by the endothecium and middle layers.
27481894	2	36	theme	xylan	461:465	arg1	epitopes					467:474	different arabinogalactan protein (AGP), pectin, xyloglucan and xylan epitopes	397:474	different arabinogalactan protein (AGP), pectin, xyloglucan and xylan epitopes in high-pressure-frozen/freeze-substituted anthers	397:525	We used immunogold labeling to study the spatial and temporal patterns of the composition and distribution of different arabinogalactan protein (AGP), pectin, xyloglucan and xylan epitopes in high-pressure-frozen/freeze-substituted anthers, quantifying and comparing their relative levels in the different anther tissues and developmental stages.
27481894	8	37	theme	anther	1525:1530	arg1	dehiscence					1532:1541	anther dehiscence	1525:1541	anther dehiscence	1525:1541	In contrast, the changes in pectin and hemicellulose epitopes suggested a specific role in anther dehiscence, facilitating anther wall weakening and rupture.
27481894	0	38	theme	Pectin	63:68	arg1	Immunolocalization					16:33	Ultrastructural Immunolocalization	0:33	Ultrastructural Immunolocalization of Arabinogalactan Protein, Pectin and Hemicellulose	0:86	Ultrastructural Immunolocalization of Arabinogalactan Protein, Pectin and Hemicellulose Epitopes Through Anther Development in Brassica napus.
27481894	6	39	contain	have	1171:1174	arg1	xylan					1165:1169	xylan	1165:1169	xylan	1165:1169	Our results suggested that AGPs, pectins, xyloglucan and xylan have specific roles during anther development.
27481894	6	39	contain	have	1171:1174	arg2	roles					1185:1189	specific roles	1176:1189	specific roles	1176:1189	Our results suggested that AGPs, pectins, xyloglucan and xylan have specific roles during anther development.
27481894	6	39	contain	have	1171:1174	arg1	pectins					1141:1147	pectins	1141:1147	pectins	1141:1147	Our results suggested that AGPs, pectins, xyloglucan and xylan have specific roles during anther development.
27481894	6	39	contain	have	1171:1174	arg1	xyloglucan					1150:1159	xyloglucan	1150:1159	xyloglucan	1150:1159	Our results suggested that AGPs, pectins, xyloglucan and xylan have specific roles during anther development.
27481894	6	39	contain	have	1171:1174	arg1	AGPs					1135:1138	AGPs	1135:1138	AGPs	1135:1138	Our results suggested that AGPs, pectins, xyloglucan and xylan have specific roles during anther development.
27481894	1	40	theme	cell	223:226	arg1	composition					233:243	cell wall composition	223:243	cell wall composition	223:243	In this work, we performed an extensive and detailed analysis of the changes in cell wall composition during Brassica napus anther development.
27481894	2	41	from	levels	569:574	arg1	tissues					600:606	the different anther tissues	579:606	the different anther tissues	579:606	We used immunogold labeling to study the spatial and temporal patterns of the composition and distribution of different arabinogalactan protein (AGP), pectin, xyloglucan and xylan epitopes in high-pressure-frozen/freeze-substituted anthers, quantifying and comparing their relative levels in the different anther tissues and developmental stages.
27481894	2	41	from	levels	569:574	arg1	stages					626:631	developmental stages	612:631	developmental stages	612:631	We used immunogold labeling to study the spatial and temporal patterns of the composition and distribution of different arabinogalactan protein (AGP), pectin, xyloglucan and xylan epitopes in high-pressure-frozen/freeze-substituted anthers, quantifying and comparing their relative levels in the different anther tissues and developmental stages.
27481894	7	42	theme	AGP	1222:1224	arg1	epitopes					1226:1233	The AGP epitopes	1218:1233	The AGP epitopes studied	1218:1241	The AGP epitopes studied appeared to belong to AGPs specifically involved in microspore differentiation, and contributed first by the tapetum and then, upon tapetal dismantling, by the endothecium and middle layers.
27481894	1	43	theme	Brassica	252:259	arg1	development					274:284	Brassica napus anther development	252:284	Brassica napus anther development	252:284	In this work, we performed an extensive and detailed analysis of the changes in cell wall composition during Brassica napus anther development.
27481894	2	44	used	used	290:293	arg2	We					287:288	We	287:288	We	287:288	We used immunogold labeling to study the spatial and temporal patterns of the composition and distribution of different arabinogalactan protein (AGP), pectin, xyloglucan and xylan epitopes in high-pressure-frozen/freeze-substituted anthers, quantifying and comparing their relative levels in the different anther tissues and developmental stages.
27481894	2	45	theme	immunogold	295:304	arg1	labeling					306:313	immunogold labeling	295:313	immunogold labeling	295:313	We used immunogold labeling to study the spatial and temporal patterns of the composition and distribution of different arabinogalactan protein (AGP), pectin, xyloglucan and xylan epitopes in high-pressure-frozen/freeze-substituted anthers, quantifying and comparing their relative levels in the different anther tissues and developmental stages.
27481894	1	46	theme	anther	267:272	arg1	development					274:284	Brassica napus anther development	252:284	Brassica napus anther development	252:284	In this work, we performed an extensive and detailed analysis of the changes in cell wall composition during Brassica napus anther development.
27481894	7	47	theme	middle	1419:1424	arg1	layers					1426:1431	middle layers	1419:1431	middle layers	1419:1431	The AGP epitopes studied appeared to belong to AGPs specifically involved in microspore differentiation, and contributed first by the tapetum and then, upon tapetal dismantling, by the endothecium and middle layers.
27481894	9	48	theme	cell	1626:1629	arg1	constituents					1636:1647	the different cell wall constituents	1612:1647	the different cell wall constituents	1612:1647	The distribution of the different cell wall constituents is regulated in a tissue- and stage-specific manner, which seems directly related to the role of each tissue at each stage.
27481894	1	49	theme	extensive	173:181	arg1	analysis					196:203	an extensive and detailed analysis	170:203	an extensive and detailed analysis of the changes in cell wall composition	170:243	In this work, we performed an extensive and detailed analysis of the changes in cell wall composition during Brassica napus anther development.
27481894	9	50	theme	constituents	1636:1647	arg1	distribution					1596:1607	The distribution	1592:1607	The distribution of the different cell wall constituents	1592:1647	The distribution of the different cell wall constituents is regulated in a tissue- and stage-specific manner, which seems directly related to the role of each tissue at each stage.
27481894	1	51	theme	detailed	187:194	arg1	analysis					196:203	an extensive and detailed analysis	170:203	an extensive and detailed analysis of the changes in cell wall composition	170:243	In this work, we performed an extensive and detailed analysis of the changes in cell wall composition during Brassica napus anther development.
27481894	7	52	theme	tapetal	1375:1381	arg1	dismantling					1383:1393	tapetal dismantling	1375:1393	tapetal dismantling	1375:1393	The AGP epitopes studied appeared to belong to AGPs specifically involved in microspore differentiation, and contributed first by the tapetum and then, upon tapetal dismantling, by the endothecium and middle layers.
27481894	2	53	from	anthers	519:525	arg1	distribution					381:392	distribution	381:392	distribution	381:392	We used immunogold labeling to study the spatial and temporal patterns of the composition and distribution of different arabinogalactan protein (AGP), pectin, xyloglucan and xylan epitopes in high-pressure-frozen/freeze-substituted anthers, quantifying and comparing their relative levels in the different anther tissues and developmental stages.
27481894	2	53	from	anthers	519:525	arg1	composition					365:375	composition	365:375	composition	365:375	We used immunogold labeling to study the spatial and temporal patterns of the composition and distribution of different arabinogalactan protein (AGP), pectin, xyloglucan and xylan epitopes in high-pressure-frozen/freeze-substituted anthers, quantifying and comparing their relative levels in the different anther tissues and developmental stages.
27481894	1	54	theme	changes	212:218	arg1	analysis					196:203	an extensive and detailed analysis	170:203	an extensive and detailed analysis of the changes in cell wall composition	170:243	In this work, we performed an extensive and detailed analysis of the changes in cell wall composition during Brassica napus anther development.
27481894	4	55	theme	labeling	891:898	arg1	pattern					900:906	a characteristic temporal and spatial labeling pattern	853:906	a characteristic temporal and spatial labeling pattern	853:906	Each cell wall epitope showed a characteristic temporal and spatial labeling pattern.
27481894	1	56	from	composition	233:243	arg1	analysis					196:203	an extensive and detailed analysis	170:203	an extensive and detailed analysis of the changes in cell wall composition	170:243	In this work, we performed an extensive and detailed analysis of the changes in cell wall composition during Brassica napus anther development.
27481894	4	57	theme	spatial	883:889	arg1	pattern					900:906	a characteristic temporal and spatial labeling pattern	853:906	a characteristic temporal and spatial labeling pattern	853:906	Each cell wall epitope showed a characteristic temporal and spatial labeling pattern.
27481894	6	58	theme	specific	1176:1183	arg1	roles					1185:1189	specific roles	1176:1189	specific roles	1176:1189	Our results suggested that AGPs, pectins, xyloglucan and xylan have specific roles during anther development.
27481894	5	59	theme	anther	1010:1015	arg1	layers					1017:1022	the outermost anther layers	996:1022	the outermost anther layers (epidermis, endothecium and middle layers)	996:1065	Microspore, pollen and tapetal cells showed similar patterns for each epitope, whereas the outermost anther layers (epidermis, endothecium and middle layers) presented remarkably different patterns.
27481894	5	59	theme	anther	1010:1015	arg1	endothecium					1036:1046	endothecium	1036:1046	endothecium	1036:1046	Microspore, pollen and tapetal cells showed similar patterns for each epitope, whereas the outermost anther layers (epidermis, endothecium and middle layers) presented remarkably different patterns.
27481894	5	59	theme	anther	1010:1015	arg1	epidermis					1025:1033	epidermis	1025:1033	epidermis	1025:1033	Microspore, pollen and tapetal cells showed similar patterns for each epitope, whereas the outermost anther layers (epidermis, endothecium and middle layers) presented remarkably different patterns.
27481894	3	60	theme	monoclonal	656:665	arg1	LM11					807:810	LM11	807:810	LM11	807:810	We used the following monoclonal antibodies: JIM13, JIM8, JIM14 and JIM16 for AGPs, LM5, LM6, JIM7, JIM5 and LM7 for pectins, CCRC-M1, CCRC-M89 and LM15 for xyloglucan, and LM11 for xylan.
27481894	3	60	theme	monoclonal	656:665	arg1	LM15					782:785	LM15	782:785	LM15	782:785	We used the following monoclonal antibodies: JIM13, JIM8, JIM14 and JIM16 for AGPs, LM5, LM6, JIM7, JIM5 and LM7 for pectins, CCRC-M1, CCRC-M89 and LM15 for xyloglucan, and LM11 for xylan.
27481894	3	60	theme	monoclonal	656:665	arg1	JIM13					679:683	JIM13	679:683	JIM13	679:683	We used the following monoclonal antibodies: JIM13, JIM8, JIM14 and JIM16 for AGPs, LM5, LM6, JIM7, JIM5 and LM7 for pectins, CCRC-M1, CCRC-M89 and LM15 for xyloglucan, and LM11 for xylan.
27481894	3	60	theme	monoclonal	656:665	arg1	JIM8					686:689	JIM8	686:689	JIM8	686:689	We used the following monoclonal antibodies: JIM13, JIM8, JIM14 and JIM16 for AGPs, LM5, LM6, JIM7, JIM5 and LM7 for pectins, CCRC-M1, CCRC-M89 and LM15 for xyloglucan, and LM11 for xylan.
27481894	3	60	theme	monoclonal	656:665	arg1	CCRC-M1					760:766	CCRC-M1	760:766	CCRC-M1	760:766	We used the following monoclonal antibodies: JIM13, JIM8, JIM14 and JIM16 for AGPs, LM5, LM6, JIM7, JIM5 and LM7 for pectins, CCRC-M1, CCRC-M89 and LM15 for xyloglucan, and LM11 for xylan.
27481894	3	60	theme	monoclonal	656:665	arg1	antibodies					667:676	the following monoclonal antibodies	642:676	the following monoclonal antibodies: JIM13, JIM8, JIM14 and JIM16 for AGPs, LM5, LM6, JIM7, JIM5 and LM7 for pectins, CCRC-M1, CCRC-M89 and LM15 for xyloglucan, and LM11 for xylan	642:820	We used the following monoclonal antibodies: JIM13, JIM8, JIM14 and JIM16 for AGPs, LM5, LM6, JIM7, JIM5 and LM7 for pectins, CCRC-M1, CCRC-M89 and LM15 for xyloglucan, and LM11 for xylan.
27481894	3	60	theme	monoclonal	656:665	arg1	JIM14					692:696	JIM14	692:696	JIM14	692:696	We used the following monoclonal antibodies: JIM13, JIM8, JIM14 and JIM16 for AGPs, LM5, LM6, JIM7, JIM5 and LM7 for pectins, CCRC-M1, CCRC-M89 and LM15 for xyloglucan, and LM11 for xylan.
27481894	3	60	theme	monoclonal	656:665	arg1	CCRC-M89					769:776	CCRC-M89	769:776	CCRC-M89	769:776	We used the following monoclonal antibodies: JIM13, JIM8, JIM14 and JIM16 for AGPs, LM5, LM6, JIM7, JIM5 and LM7 for pectins, CCRC-M1, CCRC-M89 and LM15 for xyloglucan, and LM11 for xylan.
27481894	3	60	theme	monoclonal	656:665	arg1	JIM16					702:706	JIM16	702:706	JIM16	702:706	We used the following monoclonal antibodies: JIM13, JIM8, JIM14 and JIM16 for AGPs, LM5, LM6, JIM7, JIM5 and LM7 for pectins, CCRC-M1, CCRC-M89 and LM15 for xyloglucan, and LM11 for xylan.
27481894	4	61	theme	characteristic	855:868	arg1	pattern					900:906	a characteristic temporal and spatial labeling pattern	853:906	a characteristic temporal and spatial labeling pattern	853:906	Each cell wall epitope showed a characteristic temporal and spatial labeling pattern.
27481894	2	62	dep	study	318:322	arg1	comparing					544:552	comparing	544:552	comparing their relative levels in the different anther tissues and developmental stages	544:631	We used immunogold labeling to study the spatial and temporal patterns of the composition and distribution of different arabinogalactan protein (AGP), pectin, xyloglucan and xylan epitopes in high-pressure-frozen/freeze-substituted anthers, quantifying and comparing their relative levels in the different anther tissues and developmental stages.
27481894	2	62	dep	study	318:322	arg1	quantifying					528:538	quantifying	528:538	quantifying	528:538	We used immunogold labeling to study the spatial and temporal patterns of the composition and distribution of different arabinogalactan protein (AGP), pectin, xyloglucan and xylan epitopes in high-pressure-frozen/freeze-substituted anthers, quantifying and comparing their relative levels in the different anther tissues and developmental stages.
27481894	1	63	from	analysis	196:203	arg1	composition					233:243	cell wall composition	223:243	cell wall composition	223:243	In this work, we performed an extensive and detailed analysis of the changes in cell wall composition during Brassica napus anther development.
27481894	8	64	theme	anther	1557:1562	arg1	weakening					1569:1577	anther wall weakening	1557:1577	anther wall weakening	1557:1577	In contrast, the changes in pectin and hemicellulose epitopes suggested a specific role in anther dehiscence, facilitating anther wall weakening and rupture.
27481894	0	65	theme	Anther	105:110	arg1	Development					112:122	Anther Development	105:122	Anther Development in Brassica napus	105:140	Ultrastructural Immunolocalization of Arabinogalactan Protein, Pectin and Hemicellulose Epitopes Through Anther Development in Brassica napus.
27481894	5	66	theme	different	1088:1096	arg1	patterns					1098:1105	remarkably different patterns	1077:1105	remarkably different patterns	1077:1105	Microspore, pollen and tapetal cells showed similar patterns for each epitope, whereas the outermost anther layers (epidermis, endothecium and middle layers) presented remarkably different patterns.
27481894	2	67	theme	high-pressure-frozen/freeze-substituted	479:517	arg1	anthers					519:525	high-pressure-frozen/freeze-substituted anthers	479:525	high-pressure-frozen/freeze-substituted anthers	479:525	We used immunogold labeling to study the spatial and temporal patterns of the composition and distribution of different arabinogalactan protein (AGP), pectin, xyloglucan and xylan epitopes in high-pressure-frozen/freeze-substituted anthers, quantifying and comparing their relative levels in the different anther tissues and developmental stages.
27481894	8	68	theme	pectin	1462:1467	arg1	epitopes					1487:1494	pectin and hemicellulose epitopes	1462:1494	pectin and hemicellulose epitopes	1462:1494	In contrast, the changes in pectin and hemicellulose epitopes suggested a specific role in anther dehiscence, facilitating anther wall weakening and rupture.
27481894	8	69	theme	hemicellulose	1473:1485	arg1	epitopes					1487:1494	pectin and hemicellulose epitopes	1462:1494	pectin and hemicellulose epitopes	1462:1494	In contrast, the changes in pectin and hemicellulose epitopes suggested a specific role in anther dehiscence, facilitating anther wall weakening and rupture.
27481894	5	70	theme	tapetal	932:938	arg1	cells					940:944	Microspore, pollen and tapetal cells	909:944	Microspore, pollen and tapetal cells	909:944	Microspore, pollen and tapetal cells showed similar patterns for each epitope, whereas the outermost anther layers (epidermis, endothecium and middle layers) presented remarkably different patterns.
27481894	7	71	theme	microspore	1295:1304	arg1	differentiation					1306:1320	microspore differentiation	1295:1320	microspore differentiation	1295:1320	The AGP epitopes studied appeared to belong to AGPs specifically involved in microspore differentiation, and contributed first by the tapetum and then, upon tapetal dismantling, by the endothecium and middle layers.
27481894	2	72	dep	composition	365:375	arg1	the					361:363	the	361:363	the	361:363	We used immunogold labeling to study the spatial and temporal patterns of the composition and distribution of different arabinogalactan protein (AGP), pectin, xyloglucan and xylan epitopes in high-pressure-frozen/freeze-substituted anthers, quantifying and comparing their relative levels in the different anther tissues and developmental stages.
27481894	2	73	theme	different	397:405	arg1	AGP					432:434	AGP	432:434	AGP	432:434	We used immunogold labeling to study the spatial and temporal patterns of the composition and distribution of different arabinogalactan protein (AGP), pectin, xyloglucan and xylan epitopes in high-pressure-frozen/freeze-substituted anthers, quantifying and comparing their relative levels in the different anther tissues and developmental stages.
27481894	2	73	theme	different	397:405	arg1	protein					423:429	different arabinogalactan protein	397:429	different arabinogalactan protein (AGP)	397:435	We used immunogold labeling to study the spatial and temporal patterns of the composition and distribution of different arabinogalactan protein (AGP), pectin, xyloglucan and xylan epitopes in high-pressure-frozen/freeze-substituted anthers, quantifying and comparing their relative levels in the different anther tissues and developmental stages.
27481894	8	74	theme	specific	1508:1515	arg1	role					1517:1520	a specific role	1506:1520	a specific role in anther dehiscence	1506:1541	In contrast, the changes in pectin and hemicellulose epitopes suggested a specific role in anther dehiscence, facilitating anther wall weakening and rupture.
27481894	2	75	theme	distribution	381:392	arg1	patterns					349:356	the spatial and temporal patterns	324:356	the spatial and temporal patterns of the composition and distribution of different arabinogalactan protein (AGP), pectin, xyloglucan and xylan epitopes in high-pressure-frozen/freeze-substituted anthers	324:525	We used immunogold labeling to study the spatial and temporal patterns of the composition and distribution of different arabinogalactan protein (AGP), pectin, xyloglucan and xylan epitopes in high-pressure-frozen/freeze-substituted anthers, quantifying and comparing their relative levels in the different anther tissues and developmental stages.
27481894	0	76	theme	Arabinogalactan	38:52	arg1	Protein					54:60	Arabinogalactan Protein	38:60	Arabinogalactan Protein	38:60	Ultrastructural Immunolocalization of Arabinogalactan Protein, Pectin and Hemicellulose Epitopes Through Anther Development in Brassica napus.
27481894	2	77	theme	epitopes	467:474	arg1	distribution					381:392	distribution	381:392	distribution	381:392	We used immunogold labeling to study the spatial and temporal patterns of the composition and distribution of different arabinogalactan protein (AGP), pectin, xyloglucan and xylan epitopes in high-pressure-frozen/freeze-substituted anthers, quantifying and comparing their relative levels in the different anther tissues and developmental stages.
27481894	2	77	theme	epitopes	467:474	arg1	composition					365:375	composition	365:375	composition	365:375	We used immunogold labeling to study the spatial and temporal patterns of the composition and distribution of different arabinogalactan protein (AGP), pectin, xyloglucan and xylan epitopes in high-pressure-frozen/freeze-substituted anthers, quantifying and comparing their relative levels in the different anther tissues and developmental stages.
27481894	2	78	theme	protein	423:429	arg1	epitopes					467:474	different arabinogalactan protein (AGP), pectin, xyloglucan and xylan epitopes	397:474	different arabinogalactan protein (AGP), pectin, xyloglucan and xylan epitopes in high-pressure-frozen/freeze-substituted anthers	397:525	We used immunogold labeling to study the spatial and temporal patterns of the composition and distribution of different arabinogalactan protein (AGP), pectin, xyloglucan and xylan epitopes in high-pressure-frozen/freeze-substituted anthers, quantifying and comparing their relative levels in the different anther tissues and developmental stages.
27481894	2	79	from	distribution	381:392	arg1	anthers					519:525	high-pressure-frozen/freeze-substituted anthers	479:525	high-pressure-frozen/freeze-substituted anthers	479:525	We used immunogold labeling to study the spatial and temporal patterns of the composition and distribution of different arabinogalactan protein (AGP), pectin, xyloglucan and xylan epitopes in high-pressure-frozen/freeze-substituted anthers, quantifying and comparing their relative levels in the different anther tissues and developmental stages.
27481894	2	80	theme	composition	365:375	arg1	patterns					349:356	the spatial and temporal patterns	324:356	the spatial and temporal patterns of the composition and distribution of different arabinogalactan protein (AGP), pectin, xyloglucan and xylan epitopes in high-pressure-frozen/freeze-substituted anthers	324:525	We used immunogold labeling to study the spatial and temporal patterns of the composition and distribution of different arabinogalactan protein (AGP), pectin, xyloglucan and xylan epitopes in high-pressure-frozen/freeze-substituted anthers, quantifying and comparing their relative levels in the different anther tissues and developmental stages.
27481894	2	81	theme	temporal	340:347	arg1	patterns					349:356	the spatial and temporal patterns	324:356	the spatial and temporal patterns of the composition and distribution of different arabinogalactan protein (AGP), pectin, xyloglucan and xylan epitopes in high-pressure-frozen/freeze-substituted anthers	324:525	We used immunogold labeling to study the spatial and temporal patterns of the composition and distribution of different arabinogalactan protein (AGP), pectin, xyloglucan and xylan epitopes in high-pressure-frozen/freeze-substituted anthers, quantifying and comparing their relative levels in the different anther tissues and developmental stages.
27481894	5	82	theme	similar	953:959	arg1	patterns					961:968	similar patterns	953:968	similar patterns for each epitope	953:985	Microspore, pollen and tapetal cells showed similar patterns for each epitope, whereas the outermost anther layers (epidermis, endothecium and middle layers) presented remarkably different patterns.
27481894	8	83	theme	wall	1564:1567	arg1	weakening					1569:1577	anther wall weakening	1557:1577	anther wall weakening	1557:1577	In contrast, the changes in pectin and hemicellulose epitopes suggested a specific role in anther dehiscence, facilitating anther wall weakening and rupture.
27481894	2	84	theme	spatial	328:334	arg1	patterns					349:356	the spatial and temporal patterns	324:356	the spatial and temporal patterns of the composition and distribution of different arabinogalactan protein (AGP), pectin, xyloglucan and xylan epitopes in high-pressure-frozen/freeze-substituted anthers	324:525	We used immunogold labeling to study the spatial and temporal patterns of the composition and distribution of different arabinogalactan protein (AGP), pectin, xyloglucan and xylan epitopes in high-pressure-frozen/freeze-substituted anthers, quantifying and comparing their relative levels in the different anther tissues and developmental stages.
27481894	1	85	theme	wall	228:231	arg1	composition					233:243	cell wall composition	223:243	cell wall composition	223:243	In this work, we performed an extensive and detailed analysis of the changes in cell wall composition during Brassica napus anther development.
27481894	9	86	theme	tissue	1751:1756	arg1	role					1738:1741	the role	1734:1741	the role of each tissue at each stage	1734:1770	The distribution of the different cell wall constituents is regulated in a tissue- and stage-specific manner, which seems directly related to the role of each tissue at each stage.
25842113	3	0	theme	work	483:486	arg1	aim					471:473	The aim	467:473	The aim of this work	467:486	The aim of this work was to select the best manufacturing method and technological parameters to obtain multiphase, and multifunctional, biodegradable intramedullary nails.
25842113	0	1	theme	mechanical	66:75	arg1	properties					77:86	nail's mechanical properties	59:86	nail's mechanical properties	59:86	Influence of the intramedullary nail preparation method on nail's mechanical properties and degradation rate.
25842113	9	2	theme	higher	1307:1312	arg1	fractions					1321:1329	higher volume fractions	1307:1329	higher volume fractions of fibres	1307:1339	The study showed that injection moulding provides higher uniformity and homogeneity of the particle-modified polylactide matrix, whereas hot pressing favours applying higher volume fractions of fibres and their better impregnation with the polymer matrix.
25842113	6	3	mod	modified	835:842	arg1	matrix					819:824	The polylactide matrix	803:824	The polylactide matrix	803:824	The polylactide matrix was also modified with tricalcium phosphate and gentamicin sulfate.
25842113	6	3	mod	modified	835:842	arg3	phosphate					860:868	tricalcium phosphate	849:868	tricalcium phosphate	849:868	The polylactide matrix was also modified with tricalcium phosphate and gentamicin sulfate.
25842113	6	3	mod	modified	835:842	arg3	sulfate					885:891	gentamicin sulfate	874:891	gentamicin sulfate	874:891	The polylactide matrix was also modified with tricalcium phosphate and gentamicin sulfate.
25842113	9	4	theme	injection	1162:1170	arg1	moulding					1172:1179	injection moulding	1162:1179	injection moulding	1162:1179	The study showed that injection moulding provides higher uniformity and homogeneity of the particle-modified polylactide matrix, whereas hot pressing favours applying higher volume fractions of fibres and their better impregnation with the polymer matrix.
25842113	1	5	theme	long	144:147	arg1	fractures					154:162	long bone fractures	144:162	long bone fractures	144:162	When it comes to the treatment of long bone fractures, scientists are still investigating new materials for intramedullary nails and different manufacturing methods.
25842113	1	6	theme	different	243:251	arg1	methods					267:273	different manufacturing methods	243:273	different manufacturing methods	243:273	When it comes to the treatment of long bone fractures, scientists are still investigating new materials for intramedullary nails and different manufacturing methods.
25842113	0	7	theme	degradation	92:102	arg1	rate					104:107	degradation rate	92:107	degradation rate	92:107	Influence of the intramedullary nail preparation method on nail's mechanical properties and degradation rate.
25842113	9	8	theme	volume	1314:1319	arg1	fractions					1321:1329	higher volume fractions	1307:1329	higher volume fractions of fibres	1307:1339	The study showed that injection moulding provides higher uniformity and homogeneity of the particle-modified polylactide matrix, whereas hot pressing favours applying higher volume fractions of fibres and their better impregnation with the polymer matrix.
25842113	9	9	theme	hot	1277:1279	arg1	pressing					1281:1288	hot pressing	1277:1288	hot pressing	1277:1288	The study showed that injection moulding provides higher uniformity and homogeneity of the particle-modified polylactide matrix, whereas hot pressing favours applying higher volume fractions of fibres and their better impregnation with the polymer matrix.
25842113	3	10	theme	manufacturing	511:523	arg1	method					525:530	the best manufacturing method	502:530	the best manufacturing method	502:530	The aim of this work was to select the best manufacturing method and technological parameters to obtain multiphase, and multifunctional, biodegradable intramedullary nails.
25842113	3	11	theme	technological	536:548	arg1	parameters					550:559	technological parameters	536:559	technological parameters	536:559	The aim of this work was to select the best manufacturing method and technological parameters to obtain multiphase, and multifunctional, biodegradable intramedullary nails.
25842113	1	12	theme	manufacturing	253:265	arg1	methods					267:273	different manufacturing methods	243:273	different manufacturing methods	243:273	When it comes to the treatment of long bone fractures, scientists are still investigating new materials for intramedullary nails and different manufacturing methods.
25842113	7	13	theme	different	944:952	arg1	methods					954:960	three different methods:	938:961	three different methods: forming from solution	938:983	The composite nails were manufactured using three different methods: forming from solution, injection moulding and hot pressing.
25842113	7	13	theme	different	944:952	arg1	moulding					996:1003	injection moulding	986:1003	injection moulding	986:1003	The composite nails were manufactured using three different methods: forming from solution, injection moulding and hot pressing.
25842113	7	13	theme	different	944:952	arg1	pressing					1013:1020	hot pressing	1009:1020	hot pressing	1009:1020	The composite nails were manufactured using three different methods: forming from solution, injection moulding and hot pressing.
25842113	4	14	theme	l-lactide	676:684	arg1	matrix					687:692	a poly(l-lactide) matrix	669:692	a poly(l-lactide) matrix	669:692	All composites were based on a poly(l-lactide) matrix.
25842113	6	15	theme	gentamicin	874:883	arg1	sulfate					885:891	gentamicin sulfate	874:891	gentamicin sulfate	874:891	The polylactide matrix was also modified with tricalcium phosphate and gentamicin sulfate.
25842113	8	16	theme	method	1042:1047	arg1	effect					1027:1032	The effect	1023:1032	The effect of each method of manufacturing on mechanical properties and degradation rate of the nails	1023:1123	The effect of each method of manufacturing on mechanical properties and degradation rate of the nails was evaluated.
25842113	1	17	theme	bone	149:152	arg1	fractures					154:162	long bone fractures	144:162	long bone fractures	144:162	When it comes to the treatment of long bone fractures, scientists are still investigating new materials for intramedullary nails and different manufacturing methods.
25842113	9	18	theme	fibres	1334:1339	arg1	impregnation					1358:1369	their better impregnation	1345:1369	their better impregnation with the polymer matrix	1345:1393	The study showed that injection moulding provides higher uniformity and homogeneity of the particle-modified polylactide matrix, whereas hot pressing favours applying higher volume fractions of fibres and their better impregnation with the polymer matrix.
25842113	9	18	theme	fibres	1334:1339	arg1	fractions					1321:1329	higher volume fractions	1307:1329	higher volume fractions of fibres	1307:1339	The study showed that injection moulding provides higher uniformity and homogeneity of the particle-modified polylactide matrix, whereas hot pressing favours applying higher volume fractions of fibres and their better impregnation with the polymer matrix.
25842113	9	19	with	impregnation	1358:1369	arg1	matrix					1388:1393	the polymer matrix	1376:1393	the polymer matrix	1376:1393	The study showed that injection moulding provides higher uniformity and homogeneity of the particle-modified polylactide matrix, whereas hot pressing favours applying higher volume fractions of fibres and their better impregnation with the polymer matrix.
25842113	3	20	theme	best	506:509	arg1	method					525:530	the best manufacturing method	502:530	the best manufacturing method	502:530	The aim of this work was to select the best manufacturing method and technological parameters to obtain multiphase, and multifunctional, biodegradable intramedullary nails.
25842113	1	21	theme	fractures	154:162	arg1	treatment					131:139	the treatment	127:139	the treatment of long bone fractures	127:162	When it comes to the treatment of long bone fractures, scientists are still investigating new materials for intramedullary nails and different manufacturing methods.
25842113	2	22	theme	manufacturing	429:441	arg1	range					410:414	a wide range	403:414	a wide range of potential manufacturing and processing methods	403:464	Some of the most promising materials used in the field are resorbable polymers and their composites, especially since there is a wide range of potential manufacturing and processing methods.
25842113	8	23	theme	manufacturing	1052:1064	arg1	effect					1027:1032	The effect	1023:1032	The effect of each method of manufacturing on mechanical properties and degradation rate of the nails	1023:1123	The effect of each method of manufacturing on mechanical properties and degradation rate of the nails was evaluated.
25842113	2	24	theme	potential	419:427	arg1	manufacturing					429:441	potential manufacturing	419:441	potential manufacturing	419:441	Some of the most promising materials used in the field are resorbable polymers and their composites, especially since there is a wide range of potential manufacturing and processing methods.
25842113	10	25	theme	phase	1513:1517	arg1	composition					1519:1529	the nail's desired phase composition	1494:1529	the nail's desired phase composition	1494:1529	Thus, it was concluded that the fabrication method should be individually selected dependently on the nail's desired phase composition.
25842113	0	26	theme	intramedullary	17:30	arg1	method					49:54	the intramedullary nail preparation method	13:54	the intramedullary nail preparation method	13:54	Influence of the intramedullary nail preparation method on nail's mechanical properties and degradation rate.
25842113	8	27	from	effect	1027:1032	arg1	properties					1080:1089	mechanical properties	1069:1089	mechanical properties	1069:1089	The effect of each method of manufacturing on mechanical properties and degradation rate of the nails was evaluated.
25842113	8	27	from	effect	1027:1032	arg1	rate					1107:1110	degradation rate	1095:1110	degradation rate	1095:1110	The effect of each method of manufacturing on mechanical properties and degradation rate of the nails was evaluated.
25842113	8	28	theme	mechanical	1069:1078	arg1	properties					1080:1089	mechanical properties	1069:1089	mechanical properties	1069:1089	The effect of each method of manufacturing on mechanical properties and degradation rate of the nails was evaluated.
25842113	2	29	theme	promising	293:301	arg1	materials					303:311	the most promising materials	284:311	the most promising materials used in the field	284:329	Some of the most promising materials used in the field are resorbable polymers and their composites, especially since there is a wide range of potential manufacturing and processing methods.
25842113	2	30	theme	wide	405:408	arg1	range					410:414	a wide range	403:414	a wide range of potential manufacturing and processing methods	403:464	Some of the most promising materials used in the field are resorbable polymers and their composites, especially since there is a wide range of potential manufacturing and processing methods.
25842113	7	31	theme	injection	986:994	arg1	methods					954:960	three different methods:	938:961	three different methods: forming from solution	938:983	The composite nails were manufactured using three different methods: forming from solution, injection moulding and hot pressing.
25842113	7	31	theme	injection	986:994	arg1	moulding					996:1003	injection moulding	986:1003	injection moulding	986:1003	The composite nails were manufactured using three different methods: forming from solution, injection moulding and hot pressing.
25842113	10	32	theme	desired	1505:1511	arg1	composition					1519:1529	the nail's desired phase composition	1494:1529	the nail's desired phase composition	1494:1529	Thus, it was concluded that the fabrication method should be individually selected dependently on the nail's desired phase composition.
25842113	6	33	theme	tricalcium	849:858	arg1	phosphate					860:868	tricalcium phosphate	849:868	tricalcium phosphate	849:868	The polylactide matrix was also modified with tricalcium phosphate and gentamicin sulfate.
25842113	2	34	theme	resorbable	335:344	arg1	polymers					346:353	resorbable polymers	335:353	resorbable polymers	335:353	Some of the most promising materials used in the field are resorbable polymers and their composites, especially since there is a wide range of potential manufacturing and processing methods.
25842113	2	34	theme	resorbable	335:344	arg1	materials					303:311	the most promising materials	284:311	the most promising materials used in the field	284:329	Some of the most promising materials used in the field are resorbable polymers and their composites, especially since there is a wide range of potential manufacturing and processing methods.
25842113	2	34	theme	resorbable	335:344	arg1	Some					276:279	Some	276:279	Some	276:279	Some of the most promising materials used in the field are resorbable polymers and their composites, especially since there is a wide range of potential manufacturing and processing methods.
25842113	0	35	theme	preparation	37:47	arg1	method					49:54	the intramedullary nail preparation method	13:54	the intramedullary nail preparation method	13:54	Influence of the intramedullary nail preparation method on nail's mechanical properties and degradation rate.
25842113	9	36	theme	particle-modified	1231:1247	arg1	matrix					1261:1266	the particle-modified polylactide matrix	1227:1266	the particle-modified polylactide matrix	1227:1266	The study showed that injection moulding provides higher uniformity and homogeneity of the particle-modified polylactide matrix, whereas hot pressing favours applying higher volume fractions of fibres and their better impregnation with the polymer matrix.
25842113	5	37	theme	magnesium	702:710	arg1	wires					718:722	magnesium alloy wires	702:722	magnesium alloy wires	702:722	Either magnesium alloy wires or carbon and alginate fibres were introduced in order to reinforce the nails.
25842113	2	38	theme	methods	458:464	arg1	range					410:414	a wide range	403:414	a wide range of potential manufacturing and processing methods	403:464	Some of the most promising materials used in the field are resorbable polymers and their composites, especially since there is a wide range of potential manufacturing and processing methods.
25842113	0	39	theme	nail	32:35	arg1	method					49:54	the intramedullary nail preparation method	13:54	the intramedullary nail preparation method	13:54	Influence of the intramedullary nail preparation method on nail's mechanical properties and degradation rate.
25842113	9	40	theme	polylactide	1249:1259	arg1	matrix					1261:1266	the particle-modified polylactide matrix	1227:1266	the particle-modified polylactide matrix	1227:1266	The study showed that injection moulding provides higher uniformity and homogeneity of the particle-modified polylactide matrix, whereas hot pressing favours applying higher volume fractions of fibres and their better impregnation with the polymer matrix.
25842113	9	41	theme	better	1351:1356	arg1	impregnation					1358:1369	their better impregnation	1345:1369	their better impregnation with the polymer matrix	1345:1393	The study showed that injection moulding provides higher uniformity and homogeneity of the particle-modified polylactide matrix, whereas hot pressing favours applying higher volume fractions of fibres and their better impregnation with the polymer matrix.
25842113	7	42	theme	hot	1009:1011	arg1	methods					954:960	three different methods:	938:961	three different methods: forming from solution	938:983	The composite nails were manufactured using three different methods: forming from solution, injection moulding and hot pressing.
25842113	7	42	theme	hot	1009:1011	arg1	pressing					1013:1020	hot pressing	1009:1020	hot pressing	1009:1020	The composite nails were manufactured using three different methods: forming from solution, injection moulding and hot pressing.
25842113	1	43	theme	new	200:202	arg1	materials					204:212	new materials	200:212	new materials for intramedullary nails and different manufacturing methods	200:273	When it comes to the treatment of long bone fractures, scientists are still investigating new materials for intramedullary nails and different manufacturing methods.
25842113	2	44	theme	processing	447:456	arg1	methods					458:464	processing methods	447:464	processing methods	447:464	Some of the most promising materials used in the field are resorbable polymers and their composites, especially since there is a wide range of potential manufacturing and processing methods.
25842113	5	45	theme	alloy	712:716	arg1	wires					718:722	magnesium alloy wires	702:722	magnesium alloy wires	702:722	Either magnesium alloy wires or carbon and alginate fibres were introduced in order to reinforce the nails.
25842113	8	46	theme	nails	1119:1123	arg1	properties					1080:1089	mechanical properties	1069:1089	mechanical properties	1069:1089	The effect of each method of manufacturing on mechanical properties and degradation rate of the nails was evaluated.
25842113	8	46	theme	nails	1119:1123	arg1	rate					1107:1110	degradation rate	1095:1110	degradation rate	1095:1110	The effect of each method of manufacturing on mechanical properties and degradation rate of the nails was evaluated.
25842113	0	47	theme	method	49:54	arg1	Influence					0:8	Influence	0:8	Influence of the intramedullary nail preparation method on nail's mechanical properties and degradation rate.	0:108	Influence of the intramedullary nail preparation method on nail's mechanical properties and degradation rate.
25842113	8	48	theme	degradation	1095:1105	arg1	rate					1107:1110	degradation rate	1095:1110	degradation rate	1095:1110	The effect of each method of manufacturing on mechanical properties and degradation rate of the nails was evaluated.
25842113	7	49	theme	composite	898:906	arg1	nails					908:912	The composite nails	894:912	The composite nails	894:912	The composite nails were manufactured using three different methods: forming from solution, injection moulding and hot pressing.
25842113	10	50	theme	fabrication	1428:1438	arg1	method					1440:1445	the fabrication method	1424:1445	the fabrication method	1424:1445	Thus, it was concluded that the fabrication method should be individually selected dependently on the nail's desired phase composition.
25842113	9	51	dep	higher	1190:1195	arg1	uniformity					1197:1206	uniformity	1197:1206	uniformity	1197:1206	The study showed that injection moulding provides higher uniformity and homogeneity of the particle-modified polylactide matrix, whereas hot pressing favours applying higher volume fractions of fibres and their better impregnation with the polymer matrix.
25842113	6	52	theme	polylactide	807:817	arg1	matrix					819:824	The polylactide matrix	803:824	The polylactide matrix	803:824	The polylactide matrix was also modified with tricalcium phosphate and gentamicin sulfate.
25842113	3	53	theme	intramedullary	618:631	arg1	nails					633:637	multifunctional, biodegradable intramedullary nails	587:637	multifunctional, biodegradable intramedullary nails	587:637	The aim of this work was to select the best manufacturing method and technological parameters to obtain multiphase, and multifunctional, biodegradable intramedullary nails.
25842113	9	54	with	fractions	1321:1329	arg1	matrix					1388:1393	the polymer matrix	1376:1393	the polymer matrix	1376:1393	The study showed that injection moulding provides higher uniformity and homogeneity of the particle-modified polylactide matrix, whereas hot pressing favours applying higher volume fractions of fibres and their better impregnation with the polymer matrix.
25842113	3	55	theme	multifunctional	587:601	arg1	nails					633:637	multifunctional, biodegradable intramedullary nails	587:637	multifunctional, biodegradable intramedullary nails	587:637	The aim of this work was to select the best manufacturing method and technological parameters to obtain multiphase, and multifunctional, biodegradable intramedullary nails.
25842113	3	56	dep	multifunctional	587:601	arg1	biodegradable					604:616	biodegradable	604:616	biodegradable	604:616	The aim of this work was to select the best manufacturing method and technological parameters to obtain multiphase, and multifunctional, biodegradable intramedullary nails.
25842113	1	57	theme	intramedullary	218:231	arg1	nails					233:237	intramedullary nails	218:237	intramedullary nails	218:237	When it comes to the treatment of long bone fractures, scientists are still investigating new materials for intramedullary nails and different manufacturing methods.
25842113	5	58	theme	alginate	738:745	arg1	fibres					747:752	alginate fibres	738:752	alginate fibres	738:752	Either magnesium alloy wires or carbon and alginate fibres were introduced in order to reinforce the nails.
25842113	4	59	theme	poly	671:674	arg1	matrix					687:692	a poly(l-lactide) matrix	669:692	a poly(l-lactide) matrix	669:692	All composites were based on a poly(l-lactide) matrix.
25842113	9	60	theme	polymer	1380:1386	arg1	matrix					1388:1393	the polymer matrix	1376:1393	the polymer matrix	1376:1393	The study showed that injection moulding provides higher uniformity and homogeneity of the particle-modified polylactide matrix, whereas hot pressing favours applying higher volume fractions of fibres and their better impregnation with the polymer matrix.
28088718	12	0	theme	materials	2077:2085	arg1	research					2045:2052	further research	2037:2052	further research of the same or similar materials	2037:2085	Therefore, the prepared composite is showing potential to work as a disinfectant and provide an alternative solution for water disinfection; hence this study should propel further research of the same or similar materials.
28088718	6	1	theme	flow	962:965	arg1	rate					967:970	flow rate	962:970	flow rate	962:970	The effect of the composite bed mass, initial concentration of bacteria, and flow rate on the bacterial inactivation was investigated.
28088718	4	2	theme	Cts-Bent	590:597	arg1	composites					599:608	Cts-Bent composites	590:608	Cts-Bent composites	590:608	Cts-Bent composites were synthesized using in situ cross-linking method using Bent-supported silver and zinc oxide nanoparticles.
28088718	2	3	theme	effective	379:387	arg1	way					389:391	an alternative and effective way	360:391	an alternative and effective way for the disinfection process and water treatment	360:440	The advanced use of nanomaterials is observed as an alternative and effective way for the disinfection process and water treatment as a whole.
28088718	2	3	theme	effective	379:387	arg1	use					324:326	The advanced use	311:326	The advanced use of nanomaterials	311:343	The advanced use of nanomaterials is observed as an alternative and effective way for the disinfection process and water treatment as a whole.
28088718	1	4	theme	appropriate	276:286	arg1	material					301:308	an appropriate disinfectant material	273:308	an appropriate disinfectant material	273:308	Disinfection of bacterially-contaminated drinking water requires a robust and effective technique and can be achieved by using an appropriate disinfectant material.
28088718	8	5	theme	E. coli	1221:1227	arg1	curves					1260:1265	breakthrough curves	1247:1265	breakthrough curves	1247:1265	The growth curves of E. coli were expressed as breakthrough curves, based on the logistic, Gompertz, and Boltzmann models.
28088718	8	5	theme	E. coli	1221:1227	arg1	curves					1211:1216	The growth curves	1200:1216	The growth curves of E. coli	1200:1227	The growth curves of E. coli were expressed as breakthrough curves, based on the logistic, Gompertz, and Boltzmann models.
28088718	2	6	theme	advanced	315:322	arg1	way					389:391	an alternative and effective way	360:391	an alternative and effective way for the disinfection process and water treatment	360:440	The advanced use of nanomaterials is observed as an alternative and effective way for the disinfection process and water treatment as a whole.
28088718	2	6	theme	advanced	315:322	arg1	use					324:326	The advanced use	311:326	The advanced use of nanomaterials	311:343	The advanced use of nanomaterials is observed as an alternative and effective way for the disinfection process and water treatment as a whole.
28088718	2	6	theme	advanced	315:322	arg1	whole					447:451	whole	447:451	whole	447:451	The advanced use of nanomaterials is observed as an alternative and effective way for the disinfection process and water treatment as a whole.
28088718	7	7	from	presence	1123:1130	arg1	matrix					1192:1197	the chitosan matrix	1179:1197	the chitosan matrix	1179:1197	The characterization results revealed that the composites were successfully prepared and confirmed the presence of both silver and zinc oxide nanoparticles in the chitosan matrix.
28088718	9	8	theme	bacterial	1493:1501	arg1	concentration					1503:1515	low bacterial concentration	1489:1515	low bacterial concentration	1489:1515	The breakthrough time and processed volume of treated water at breakthrough were used as performance indicators, which revealed that the composites performed best at low bacterial concentration and flow rate and with substantial bed mass.
28088718	8	9	theme	breakthrough	1247:1258	arg1	curves					1260:1265	breakthrough curves	1247:1265	breakthrough curves	1247:1265	The growth curves of E. coli were expressed as breakthrough curves, based on the logistic, Gompertz, and Boltzmann models.
28088718	8	9	theme	breakthrough	1247:1258	arg1	curves					1211:1216	The growth curves	1200:1216	The growth curves of E. coli	1200:1227	The growth curves of E. coli were expressed as breakthrough curves, based on the logistic, Gompertz, and Boltzmann models.
28088718	6	10	theme	initial	923:929	arg1	concentration					931:943	initial concentration	923:943	initial concentration of bacteria	923:955	The effect of the composite bed mass, initial concentration of bacteria, and flow rate on the bacterial inactivation was investigated.
28088718	4	11	theme	oxide	699:703	arg1	nanoparticles					705:717	Bent-supported silver and zinc oxide nanoparticles	668:717	nanoparticles	705:717	Cts-Bent composites were synthesized using in situ cross-linking method using Bent-supported silver and zinc oxide nanoparticles.
28088718	11	12	theme	sigmoidal	1846:1854	arg1	pattern					1856:1862	a sigmoidal pattern	1844:1862	a sigmoidal pattern	1844:1862	All the models were suitable for adequately describing and reproducing the experimental data with a sigmoidal pattern.
28088718	7	13	theme	zinc	1151:1154	arg1	oxide					1156:1160	zinc oxide	1151:1160	zinc oxide	1151:1160	The characterization results revealed that the composites were successfully prepared and confirmed the presence of both silver and zinc oxide nanoparticles in the chitosan matrix.
28088718	7	14	theme	silver	1140:1145	arg1	nanoparticles					1162:1174	both silver and zinc oxide nanoparticles	1135:1174	both silver and zinc oxide nanoparticles	1135:1174	The characterization results revealed that the composites were successfully prepared and confirmed the presence of both silver and zinc oxide nanoparticles in the chitosan matrix.
28088718	3	15	theme	fixed	572:576	arg1	column					582:587	a fixed bed column	570:587	a fixed bed column	570:587	Hence, the inactivation of Escherichia coli (E. coli) using chitosan-Bentonite (Cts-Bent) composites was studied in a fixed bed column.
28088718	9	16	used	used	1404:1407	arg2	breakthrough					1386:1397	breakthrough	1386:1397	breakthrough	1386:1397	The breakthrough time and processed volume of treated water at breakthrough were used as performance indicators, which revealed that the composites performed best at low bacterial concentration and flow rate and with substantial bed mass.
28088718	9	16	used	used	1404:1407	arg2	indicators					1424:1433	performance indicators	1412:1433	performance indicators	1412:1433	The breakthrough time and processed volume of treated water at breakthrough were used as performance indicators, which revealed that the composites performed best at low bacterial concentration and flow rate and with substantial bed mass.
28088718	9	16	used	used	1404:1407	arg2	volume					1359:1364	processed volume	1349:1364	processed volume of treated water at breakthrough	1349:1397	The breakthrough time and processed volume of treated water at breakthrough were used as performance indicators, which revealed that the composites performed best at low bacterial concentration and flow rate and with substantial bed mass.
28088718	9	16	used	used	1404:1407	arg2	time					1340:1343	The breakthrough time	1323:1343	The breakthrough time	1323:1343	The breakthrough time and processed volume of treated water at breakthrough were used as performance indicators, which revealed that the composites performed best at low bacterial concentration and flow rate and with substantial bed mass.
28088718	7	17	attach	presence	1123:1130	arg2	nanoparticles					1162:1174	both silver and zinc oxide nanoparticles	1135:1174	both silver and zinc oxide nanoparticles	1135:1174	The characterization results revealed that the composites were successfully prepared and confirmed the presence of both silver and zinc oxide nanoparticles in the chitosan matrix.
28088718	7	17	attach	presence	1123:1130	arg1	matrix					1192:1197	the chitosan matrix	1179:1197	the chitosan matrix	1179:1197	The characterization results revealed that the composites were successfully prepared and confirmed the presence of both silver and zinc oxide nanoparticles in the chitosan matrix.
28088718	4	18	theme	Bent-supported	668:681	arg1	silver					683:688	Bent-supported silver and zinc oxide nanoparticles	668:717	silver	683:688	Cts-Bent composites were synthesized using in situ cross-linking method using Bent-supported silver and zinc oxide nanoparticles.
28088718	8	19	theme	logistic	1281:1288	arg1	Gompertz					1291:1298	the logistic, Gompertz, and Boltzmann models	1277:1320	Gompertz	1291:1298	The growth curves of E. coli were expressed as breakthrough curves, based on the logistic, Gompertz, and Boltzmann models.
28088718	6	20	theme	bacterial	979:987	arg1	inactivation					989:1000	the bacterial inactivation	975:1000	the bacterial inactivation	975:1000	The effect of the composite bed mass, initial concentration of bacteria, and flow rate on the bacterial inactivation was investigated.
28088718	9	21	theme	bed	1552:1554	arg1	mass					1556:1559	substantial bed mass	1540:1559	substantial bed mass	1540:1559	The breakthrough time and processed volume of treated water at breakthrough were used as performance indicators, which revealed that the composites performed best at low bacterial concentration and flow rate and with substantial bed mass.
28088718	5	22	dep	transform	767:775	arg1	infrared					777:784	infrared	777:784	transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, and energy dispersive spectroscopy	767:882	These composites were characterized by Fourier transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, and energy dispersive spectroscopy.
28088718	9	23	theme	breakthrough	1327:1338	arg1	indicators					1424:1433	performance indicators	1412:1433	performance indicators	1412:1433	The breakthrough time and processed volume of treated water at breakthrough were used as performance indicators, which revealed that the composites performed best at low bacterial concentration and flow rate and with substantial bed mass.
28088718	9	23	theme	breakthrough	1327:1338	arg1	time					1340:1343	The breakthrough time	1323:1343	The breakthrough time	1323:1343	The breakthrough time and processed volume of treated water at breakthrough were used as performance indicators, which revealed that the composites performed best at low bacterial concentration and flow rate and with substantial bed mass.
28088718	9	23	theme	breakthrough	1327:1338	arg1	volume					1359:1364	processed volume	1349:1364	processed volume of treated water at breakthrough	1349:1397	The breakthrough time and processed volume of treated water at breakthrough were used as performance indicators, which revealed that the composites performed best at low bacterial concentration and flow rate and with substantial bed mass.
28088718	7	24	theme	characterization	1024:1039	arg1	results					1041:1047	The characterization results	1020:1047	The characterization results	1020:1047	The characterization results revealed that the composites were successfully prepared and confirmed the presence of both silver and zinc oxide nanoparticles in the chitosan matrix.
28088718	1	25	theme	bacterially-contaminated	162:185	arg1	water					196:200	bacterially-contaminated drinking water	162:200	bacterially-contaminated drinking water	162:200	Disinfection of bacterially-contaminated drinking water requires a robust and effective technique and can be achieved by using an appropriate disinfectant material.
28088718	11	26	theme	experimental	1821:1832	arg1	data					1834:1837	the experimental data	1817:1837	the experimental data	1817:1837	All the models were suitable for adequately describing and reproducing the experimental data with a sigmoidal pattern.
28088718	0	27	theme	chitosan-bentonite	72:89	arg1	composites					91:100	chitosan-bentonite composites	72:100	chitosan-bentonite composites	72:100	Highly efficient inactivation of bacteria found in drinking water using chitosan-bentonite composites: Modelling and breakthrough curve analysis.
28088718	1	28	theme	water	196:200	arg1	Disinfection					146:157	Disinfection	146:157	Disinfection of bacterially-contaminated drinking water	146:200	Disinfection of bacterially-contaminated drinking water requires a robust and effective technique and can be achieved by using an appropriate disinfectant material.
28088718	2	29	theme	water	426:430	arg1	treatment					432:440	water treatment	426:440	water treatment	426:440	The advanced use of nanomaterials is observed as an alternative and effective way for the disinfection process and water treatment as a whole.
28088718	9	30	theme	treated	1369:1375	arg1	water					1377:1381	treated water	1369:1381	treated water	1369:1381	The breakthrough time and processed volume of treated water at breakthrough were used as performance indicators, which revealed that the composites performed best at low bacterial concentration and flow rate and with substantial bed mass.
28088718	5	31	theme	dispersive	860:869	arg1	spectroscopy					871:882	energy dispersive spectroscopy	853:882	energy dispersive spectroscopy	853:882	These composites were characterized by Fourier transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, and energy dispersive spectroscopy.
28088718	9	32	from	breakthrough	1386:1397	arg1	indicators					1424:1433	performance indicators	1412:1433	performance indicators	1412:1433	The breakthrough time and processed volume of treated water at breakthrough were used as performance indicators, which revealed that the composites performed best at low bacterial concentration and flow rate and with substantial bed mass.
28088718	9	32	from	breakthrough	1386:1397	arg1	time					1340:1343	The breakthrough time	1323:1343	The breakthrough time	1323:1343	The breakthrough time and processed volume of treated water at breakthrough were used as performance indicators, which revealed that the composites performed best at low bacterial concentration and flow rate and with substantial bed mass.
28088718	9	32	from	breakthrough	1386:1397	arg1	volume					1359:1364	processed volume	1349:1364	processed volume of treated water at breakthrough	1349:1397	The breakthrough time and processed volume of treated water at breakthrough were used as performance indicators, which revealed that the composites performed best at low bacterial concentration and flow rate and with substantial bed mass.
28088718	0	33	theme	bacteria	33:40	arg1	inactivation					17:28	Highly efficient inactivation	0:28	Highly efficient inactivation of bacteria found in drinking water using chitosan-bentonite composites: Modelling and breakthrough curve analysis.	0:144	Highly efficient inactivation of bacteria found in drinking water using chitosan-bentonite composites: Modelling and breakthrough curve analysis.
28088718	6	34	theme	bed	913:915	arg1	mass					917:920	the composite bed mass	899:920	the composite bed mass	899:920	The effect of the composite bed mass, initial concentration of bacteria, and flow rate on the bacterial inactivation was investigated.
28088718	12	35	theme	prepared	1880:1887	arg1	composite					1889:1897	the prepared composite	1876:1897	the prepared composite	1876:1897	Therefore, the prepared composite is showing potential to work as a disinfectant and provide an alternative solution for water disinfection; hence this study should propel further research of the same or similar materials.
28088718	7	36	theme	nanoparticles	1162:1174	arg1	presence					1123:1130	the presence	1119:1130	the presence of both silver and zinc oxide nanoparticles in the chitosan matrix	1119:1197	The characterization results revealed that the composites were successfully prepared and confirmed the presence of both silver and zinc oxide nanoparticles in the chitosan matrix.
28088718	10	37	theme	chitosan	1566:1573	arg1	composites					1575:1584	The chitosan composites	1562:1584	The chitosan composites	1562:1584	The chitosan composites were found to be highly effective, which was demonstrated when no bacteria were observed in the effluent sample within the first 27 h of analysing river water.
28088718	10	37	theme	chitosan	1566:1573	arg1	effective					1610:1618	effective	1610:1618	effective	1610:1618	The chitosan composites were found to be highly effective, which was demonstrated when no bacteria were observed in the effluent sample within the first 27 h of analysing river water.
28088718	0	38	dep	inactivation	17:28	arg1	Modelling					103:111	Modelling	103:111	Modelling	103:111	Highly efficient inactivation of bacteria found in drinking water using chitosan-bentonite composites: Modelling and breakthrough curve analysis.
28088718	0	38	dep	inactivation	17:28	arg1	analysis					136:143	breakthrough curve analysis	117:143	breakthrough curve analysis	117:143	Highly efficient inactivation of bacteria found in drinking water using chitosan-bentonite composites: Modelling and breakthrough curve analysis.
28088718	3	39	theme	Cts-Bent	534:541	arg1	composites					544:553	chitosan-Bentonite (Cts-Bent) composites	514:553	chitosan-Bentonite (Cts-Bent) composites	514:553	Hence, the inactivation of Escherichia coli (E. coli) using chitosan-Bentonite (Cts-Bent) composites was studied in a fixed bed column.
28088718	12	40	theme	same	2061:2064	arg1	materials					2077:2085	the same or similar materials	2057:2085	the same or similar materials	2057:2085	Therefore, the prepared composite is showing potential to work as a disinfectant and provide an alternative solution for water disinfection; hence this study should propel further research of the same or similar materials.
28088718	6	41	theme	rate	967:970	arg1	effect					889:894	The effect	885:894	The effect of the composite bed mass, initial concentration of bacteria, and flow rate on the bacterial inactivation	885:1000	The effect of the composite bed mass, initial concentration of bacteria, and flow rate on the bacterial inactivation was investigated.
28088718	4	42	theme	in	633:634	arg1	method					655:660	in situ cross-linking method	633:660	in situ cross-linking method using Bent-supported silver and zinc oxide nanoparticles	633:717	Cts-Bent composites were synthesized using in situ cross-linking method using Bent-supported silver and zinc oxide nanoparticles.
28088718	7	43	theme	chitosan	1183:1190	arg1	matrix					1192:1197	the chitosan matrix	1179:1197	the chitosan matrix	1179:1197	The characterization results revealed that the composites were successfully prepared and confirmed the presence of both silver and zinc oxide nanoparticles in the chitosan matrix.
28088718	3	44	theme	chitosan-Bentonite	514:531	arg1	composites					544:553	chitosan-Bentonite (Cts-Bent) composites	514:553	chitosan-Bentonite (Cts-Bent) composites	514:553	Hence, the inactivation of Escherichia coli (E. coli) using chitosan-Bentonite (Cts-Bent) composites was studied in a fixed bed column.
28088718	12	45	theme	similar	2069:2075	arg1	materials					2077:2085	the same or similar materials	2057:2085	the same or similar materials	2057:2085	Therefore, the prepared composite is showing potential to work as a disinfectant and provide an alternative solution for water disinfection; hence this study should propel further research of the same or similar materials.
28088718	2	46	theme	disinfection	401:412	arg1	process					414:420	the disinfection process	397:420	the disinfection process	397:420	The advanced use of nanomaterials is observed as an alternative and effective way for the disinfection process and water treatment as a whole.
28088718	1	47	theme	disinfectant	288:299	arg1	material					301:308	an appropriate disinfectant material	273:308	an appropriate disinfectant material	273:308	Disinfection of bacterially-contaminated drinking water requires a robust and effective technique and can be achieved by using an appropriate disinfectant material.
28088718	12	48	theme	further	2037:2043	arg1	research					2045:2052	further research	2037:2052	further research of the same or similar materials	2037:2085	Therefore, the prepared composite is showing potential to work as a disinfectant and provide an alternative solution for water disinfection; hence this study should propel further research of the same or similar materials.
28088718	6	49	theme	bacteria	948:955	arg1	mass					917:920	the composite bed mass	899:920	the composite bed mass	899:920	The effect of the composite bed mass, initial concentration of bacteria, and flow rate on the bacterial inactivation was investigated.
28088718	6	49	theme	bacteria	948:955	arg1	concentration					931:943	initial concentration	923:943	initial concentration of bacteria	923:955	The effect of the composite bed mass, initial concentration of bacteria, and flow rate on the bacterial inactivation was investigated.
28088718	6	49	theme	bacteria	948:955	arg1	rate					967:970	flow rate	962:970	flow rate	962:970	The effect of the composite bed mass, initial concentration of bacteria, and flow rate on the bacterial inactivation was investigated.
28088718	7	50	theme	oxide	1156:1160	arg1	nanoparticles					1162:1174	both silver and zinc oxide nanoparticles	1135:1174	both silver and zinc oxide nanoparticles	1135:1174	The characterization results revealed that the composites were successfully prepared and confirmed the presence of both silver and zinc oxide nanoparticles in the chitosan matrix.
28088718	9	51	theme	low	1489:1491	arg1	concentration					1503:1515	low bacterial concentration	1489:1515	low bacterial concentration	1489:1515	The breakthrough time and processed volume of treated water at breakthrough were used as performance indicators, which revealed that the composites performed best at low bacterial concentration and flow rate and with substantial bed mass.
28088718	6	52	theme	concentration	931:943	arg1	effect					889:894	The effect	885:894	The effect of the composite bed mass, initial concentration of bacteria, and flow rate on the bacterial inactivation	885:1000	The effect of the composite bed mass, initial concentration of bacteria, and flow rate on the bacterial inactivation was investigated.
28088718	5	53	theme	scanning	819:826	arg1	microscopy					837:846	scanning electron microscopy	819:846	scanning electron microscopy	819:846	These composites were characterized by Fourier transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, and energy dispersive spectroscopy.
28088718	3	54	theme	bed	578:580	arg1	column					582:587	a fixed bed column	570:587	a fixed bed column	570:587	Hence, the inactivation of Escherichia coli (E. coli) using chitosan-Bentonite (Cts-Bent) composites was studied in a fixed bed column.
28088718	1	55	theme	robust	213:218	arg1	technique					234:242	a robust and effective technique	211:242	a robust and effective technique	211:242	Disinfection of bacterially-contaminated drinking water requires a robust and effective technique and can be achieved by using an appropriate disinfectant material.
28088718	4	56	theme	zinc	694:697	arg1	nanoparticles					705:717	Bent-supported silver and zinc oxide nanoparticles	668:717	nanoparticles	705:717	Cts-Bent composites were synthesized using in situ cross-linking method using Bent-supported silver and zinc oxide nanoparticles.
28088718	2	57	theme	nanomaterials	331:343	arg1	way					389:391	an alternative and effective way	360:391	an alternative and effective way for the disinfection process and water treatment	360:440	The advanced use of nanomaterials is observed as an alternative and effective way for the disinfection process and water treatment as a whole.
28088718	2	57	theme	nanomaterials	331:343	arg1	use					324:326	The advanced use	311:326	The advanced use of nanomaterials	311:343	The advanced use of nanomaterials is observed as an alternative and effective way for the disinfection process and water treatment as a whole.
28088718	2	57	theme	nanomaterials	331:343	arg1	whole					447:451	whole	447:451	whole	447:451	The advanced use of nanomaterials is observed as an alternative and effective way for the disinfection process and water treatment as a whole.
28088718	1	58	theme	effective	224:232	arg1	technique					234:242	a robust and effective technique	211:242	a robust and effective technique	211:242	Disinfection of bacterially-contaminated drinking water requires a robust and effective technique and can be achieved by using an appropriate disinfectant material.
28088718	4	59	theme	cross-linking	641:653	arg1	method					655:660	in situ cross-linking method	633:660	in situ cross-linking method using Bent-supported silver and zinc oxide nanoparticles	633:717	Cts-Bent composites were synthesized using in situ cross-linking method using Bent-supported silver and zinc oxide nanoparticles.
28088718	6	60	from	effect	889:894	arg1	inactivation					989:1000	the bacterial inactivation	975:1000	the bacterial inactivation	975:1000	The effect of the composite bed mass, initial concentration of bacteria, and flow rate on the bacterial inactivation was investigated.
28088718	12	61	theme	water	1986:1990	arg1	disinfection					1992:2003	water disinfection	1986:2003	water disinfection	1986:2003	Therefore, the prepared composite is showing potential to work as a disinfectant and provide an alternative solution for water disinfection; hence this study should propel further research of the same or similar materials.
28088718	8	62	theme	growth	1204:1209	arg1	curves					1260:1265	breakthrough curves	1247:1265	breakthrough curves	1247:1265	The growth curves of E. coli were expressed as breakthrough curves, based on the logistic, Gompertz, and Boltzmann models.
28088718	8	62	theme	growth	1204:1209	arg1	curves					1211:1216	The growth curves	1200:1216	The growth curves of E. coli	1200:1227	The growth curves of E. coli were expressed as breakthrough curves, based on the logistic, Gompertz, and Boltzmann models.
28088718	9	63	theme	substantial	1540:1550	arg1	mass					1556:1559	substantial bed mass	1540:1559	substantial bed mass	1540:1559	The breakthrough time and processed volume of treated water at breakthrough were used as performance indicators, which revealed that the composites performed best at low bacterial concentration and flow rate and with substantial bed mass.
28088718	4	64	dep	in	633:634	arg1	situ					636:639	situ	636:639	situ	636:639	Cts-Bent composites were synthesized using in situ cross-linking method using Bent-supported silver and zinc oxide nanoparticles.
28088718	5	65	theme	X-ray	800:804	arg1	diffraction					806:816	X-ray diffraction	800:816	X-ray diffraction	800:816	These composites were characterized by Fourier transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, and energy dispersive spectroscopy.
28088718	9	66	theme	flow	1521:1524	arg1	rate					1526:1529	flow rate	1521:1529	flow rate	1521:1529	The breakthrough time and processed volume of treated water at breakthrough were used as performance indicators, which revealed that the composites performed best at low bacterial concentration and flow rate and with substantial bed mass.
28088718	5	67	dep	Fourier	759:765	arg1	transform					767:775	transform	767:775	transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, and energy dispersive spectroscopy	767:882	These composites were characterized by Fourier transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, and energy dispersive spectroscopy.
28088718	12	68	theme	alternative	1961:1971	arg1	solution					1973:1980	an alternative solution	1958:1980	an alternative solution for water disinfection	1958:2003	Therefore, the prepared composite is showing potential to work as a disinfectant and provide an alternative solution for water disinfection; hence this study should propel further research of the same or similar materials.
28088718	12	68	theme	alternative	1961:1971	arg1	disinfectant					1933:1944	a disinfectant	1931:1944	a disinfectant	1931:1944	Therefore, the prepared composite is showing potential to work as a disinfectant and provide an alternative solution for water disinfection; hence this study should propel further research of the same or similar materials.
28088718	1	69	theme	drinking	187:194	arg1	water					196:200	bacterially-contaminated drinking water	162:200	bacterially-contaminated drinking water	162:200	Disinfection of bacterially-contaminated drinking water requires a robust and effective technique and can be achieved by using an appropriate disinfectant material.
28088718	0	70	theme	curve	130:134	arg1	analysis					136:143	breakthrough curve analysis	117:143	breakthrough curve analysis	117:143	Highly efficient inactivation of bacteria found in drinking water using chitosan-bentonite composites: Modelling and breakthrough curve analysis.
28088718	9	71	theme	processed	1349:1357	arg1	indicators					1424:1433	performance indicators	1412:1433	performance indicators	1412:1433	The breakthrough time and processed volume of treated water at breakthrough were used as performance indicators, which revealed that the composites performed best at low bacterial concentration and flow rate and with substantial bed mass.
28088718	9	71	theme	processed	1349:1357	arg1	time					1340:1343	The breakthrough time	1323:1343	The breakthrough time	1323:1343	The breakthrough time and processed volume of treated water at breakthrough were used as performance indicators, which revealed that the composites performed best at low bacterial concentration and flow rate and with substantial bed mass.
28088718	9	71	theme	processed	1349:1357	arg1	volume					1359:1364	processed volume	1349:1364	processed volume of treated water at breakthrough	1349:1397	The breakthrough time and processed volume of treated water at breakthrough were used as performance indicators, which revealed that the composites performed best at low bacterial concentration and flow rate and with substantial bed mass.
28088718	0	72	theme	breakthrough	117:128	arg1	analysis					136:143	breakthrough curve analysis	117:143	breakthrough curve analysis	117:143	Highly efficient inactivation of bacteria found in drinking water using chitosan-bentonite composites: Modelling and breakthrough curve analysis.
28088718	8	73	theme	Boltzmann	1305:1313	arg1	models					1315:1320	the logistic, Gompertz, and Boltzmann models	1277:1320	models	1315:1320	The growth curves of E. coli were expressed as breakthrough curves, based on the logistic, Gompertz, and Boltzmann models.
28088718	5	74	theme	energy	853:858	arg1	spectroscopy					871:882	energy dispersive spectroscopy	853:882	energy dispersive spectroscopy	853:882	These composites were characterized by Fourier transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, and energy dispersive spectroscopy.
28088718	9	75	theme	water	1377:1381	arg1	indicators					1424:1433	performance indicators	1412:1433	performance indicators	1412:1433	The breakthrough time and processed volume of treated water at breakthrough were used as performance indicators, which revealed that the composites performed best at low bacterial concentration and flow rate and with substantial bed mass.
28088718	9	75	theme	water	1377:1381	arg1	time					1340:1343	The breakthrough time	1323:1343	The breakthrough time	1323:1343	The breakthrough time and processed volume of treated water at breakthrough were used as performance indicators, which revealed that the composites performed best at low bacterial concentration and flow rate and with substantial bed mass.
28088718	9	75	theme	water	1377:1381	arg1	volume					1359:1364	processed volume	1349:1364	processed volume of treated water at breakthrough	1349:1397	The breakthrough time and processed volume of treated water at breakthrough were used as performance indicators, which revealed that the composites performed best at low bacterial concentration and flow rate and with substantial bed mass.
28088718	10	76	theme	effluent	1682:1689	arg1	sample					1691:1696	the effluent sample	1678:1696	the effluent sample within the first 27 h of analysing river water	1678:1743	The chitosan composites were found to be highly effective, which was demonstrated when no bacteria were observed in the effluent sample within the first 27 h of analysing river water.
28088718	3	77	theme	coli	493:496	arg1	inactivation					465:476	the inactivation	461:476	the inactivation of Escherichia coli (E. coli) using chitosan-Bentonite (Cts-Bent) composites	461:553	Hence, the inactivation of Escherichia coli (E. coli) using chitosan-Bentonite (Cts-Bent) composites was studied in a fixed bed column.
28088718	5	78	theme	electron	828:835	arg1	microscopy					837:846	scanning electron microscopy	819:846	scanning electron microscopy	819:846	These composites were characterized by Fourier transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, and energy dispersive spectroscopy.
28088718	6	79	theme	mass	917:920	arg1	effect					889:894	The effect	885:894	The effect of the composite bed mass, initial concentration of bacteria, and flow rate on the bacterial inactivation	885:1000	The effect of the composite bed mass, initial concentration of bacteria, and flow rate on the bacterial inactivation was investigated.
28088718	10	80	theme	first	1709:1713	arg1	27 h					1715:1718	the first 27 h	1705:1718	the first 27 h of analysing river water	1705:1743	The chitosan composites were found to be highly effective, which was demonstrated when no bacteria were observed in the effluent sample within the first 27 h of analysing river water.
28088718	9	81	theme	performance	1412:1422	arg1	indicators					1424:1433	performance indicators	1412:1433	performance indicators	1412:1433	The breakthrough time and processed volume of treated water at breakthrough were used as performance indicators, which revealed that the composites performed best at low bacterial concentration and flow rate and with substantial bed mass.
28088718	9	81	theme	performance	1412:1422	arg1	time					1340:1343	The breakthrough time	1323:1343	The breakthrough time	1323:1343	The breakthrough time and processed volume of treated water at breakthrough were used as performance indicators, which revealed that the composites performed best at low bacterial concentration and flow rate and with substantial bed mass.
28088718	9	81	theme	performance	1412:1422	arg1	volume					1359:1364	processed volume	1349:1364	processed volume of treated water at breakthrough	1349:1397	The breakthrough time and processed volume of treated water at breakthrough were used as performance indicators, which revealed that the composites performed best at low bacterial concentration and flow rate and with substantial bed mass.
28088718	6	82	theme	composite	903:911	arg1	mass					917:920	the composite bed mass	899:920	the composite bed mass	899:920	The effect of the composite bed mass, initial concentration of bacteria, and flow rate on the bacterial inactivation was investigated.
28088718	10	83	located	observed	1666:1673	arg2	bacteria					1652:1659	no bacteria	1649:1659	no bacteria	1649:1659	The chitosan composites were found to be highly effective, which was demonstrated when no bacteria were observed in the effluent sample within the first 27 h of analysing river water.
28088718	10	83	located	observed	1666:1673	arg1	sample					1691:1696	the effluent sample	1678:1696	the effluent sample within the first 27 h of analysing river water	1678:1743	The chitosan composites were found to be highly effective, which was demonstrated when no bacteria were observed in the effluent sample within the first 27 h of analysing river water.
28088718	2	84	theme	alternative	363:373	arg1	way					389:391	an alternative and effective way	360:391	an alternative and effective way for the disinfection process and water treatment	360:440	The advanced use of nanomaterials is observed as an alternative and effective way for the disinfection process and water treatment as a whole.
28088718	2	84	theme	alternative	363:373	arg1	use					324:326	The advanced use	311:326	The advanced use of nanomaterials	311:343	The advanced use of nanomaterials is observed as an alternative and effective way for the disinfection process and water treatment as a whole.
28088718	10	85	theme	river	1733:1737	arg1	water					1739:1743	river water	1733:1743	river water	1733:1743	The chitosan composites were found to be highly effective, which was demonstrated when no bacteria were observed in the effluent sample within the first 27 h of analysing river water.
28088718	0	86	theme	efficient	7:15	arg1	inactivation					17:28	Highly efficient inactivation	0:28	Highly efficient inactivation of bacteria found in drinking water using chitosan-bentonite composites: Modelling and breakthrough curve analysis.	0:144	Highly efficient inactivation of bacteria found in drinking water using chitosan-bentonite composites: Modelling and breakthrough curve analysis.
24299772	2	0	theme	linkage	418:424	arg1	composition					426:436	linkage composition	418:436	linkage composition	418:436	The resultant products were analysed according to the sugars and linkage composition and also by electrospray mass spectrometry.
24299772	3	1	theme	≤	625:625	arg1	°C					631:632	T ≤ 180 °C	623:632	T ≤ 180 °C	623:632	Galactomannans did not loss mass at T ≤ 200 °C during exposures up to 3h whereas the arabinogalactans showed that thermal stability only for T ≤ 180 °C.
24299772	1	2	theme	coffee	156:161	arg1	galactomannans					177:190	spent coffee grounds (SCG) galactomannans	150:190	spent coffee grounds (SCG) galactomannans	150:190	In order to better understand the thermal stability of spent coffee grounds (SCG) galactomannans and arabinogalactans and the reactions that can occur upon roasting, long term isothermal exposures, up to 3h, were performed at 160, 180, 200, 220, and 240 °C.
24299772	6	3	from	end	1117:1119	arg1	Depolymerisation					1044:1059	Depolymerisation	1044:1059	Depolymerisation	1044:1059	Depolymerisation and formation of anhydrohexose residues at the reducing end and mannose-glucose isomerisation were also observed.
24299772	6	3	from	end	1117:1119	arg1	isomerisation					1141:1153	mannose-glucose isomerisation	1125:1153	mannose-glucose isomerisation	1125:1153	Depolymerisation and formation of anhydrohexose residues at the reducing end and mannose-glucose isomerisation were also observed.
24299772	6	3	from	end	1117:1119	arg1	formation					1065:1073	formation	1065:1073	formation	1065:1073	Depolymerisation and formation of anhydrohexose residues at the reducing end and mannose-glucose isomerisation were also observed.
24299772	6	3	from	end	1117:1119	arg1	residues					1092:1099	anhydrohexose residues	1078:1099	anhydrohexose residues at the reducing end	1078:1119	Depolymerisation and formation of anhydrohexose residues at the reducing end and mannose-glucose isomerisation were also observed.
24299772	1	4	theme	grounds	163:169	arg1	galactomannans					177:190	spent coffee grounds (SCG) galactomannans	150:190	spent coffee grounds (SCG) galactomannans	150:190	In order to better understand the thermal stability of spent coffee grounds (SCG) galactomannans and arabinogalactans and the reactions that can occur upon roasting, long term isothermal exposures, up to 3h, were performed at 160, 180, 200, 220, and 240 °C.
24299772	5	5	link	3,4,6-linked	937:948	arg1	2,3-					880:883	2,3-	880:883	2,3-	880:883	The roasting of galactomannans promoted the formation of new glycosidic linkages, with occurrence of 2-, 6-, 2,3-, 2,6-, 3,6-, 2,3,6-, 3,4,6-linked mannose residues, 3,4,6-linked galactose residues, and terminally-linked glucose residues, observed by methylation analysis.
24299772	5	5	link	3,4,6-linked	937:948	arg1	residues					960:967	3,4,6-linked galactose residues	937:967	3,4,6-linked galactose residues	937:967	The roasting of galactomannans promoted the formation of new glycosidic linkages, with occurrence of 2-, 6-, 2,3-, 2,6-, 3,6-, 2,3,6-, 3,4,6-linked mannose residues, 3,4,6-linked galactose residues, and terminally-linked glucose residues, observed by methylation analysis.
24299772	2	6	theme	mass	463:466	arg1	spectrometry					468:479	electrospray mass spectrometry	450:479	electrospray mass spectrometry	450:479	The resultant products were analysed according to the sugars and linkage composition and also by electrospray mass spectrometry.
24299772	5	7	theme	methylation	1022:1032	arg1	analysis					1034:1041	methylation analysis	1022:1041	methylation analysis	1022:1041	The roasting of galactomannans promoted the formation of new glycosidic linkages, with occurrence of 2-, 6-, 2,3-, 2,6-, 3,6-, 2,3,6-, 3,4,6-linked mannose residues, 3,4,6-linked galactose residues, and terminally-linked glucose residues, observed by methylation analysis.
24299772	2	8	dep	sugars	407:412	arg1	the					403:405	the	403:405	the	403:405	The resultant products were analysed according to the sugars and linkage composition and also by electrospray mass spectrometry.
24299772	6	9	theme	mannose-glucose	1125:1139	arg1	isomerisation					1141:1153	mannose-glucose isomerisation	1125:1153	mannose-glucose isomerisation	1125:1153	Depolymerisation and formation of anhydrohexose residues at the reducing end and mannose-glucose isomerisation were also observed.
24299772	4	10	theme	estimated	667:675	arg1	energies					688:695	the estimated activation energies	663:695	the estimated activation energies of their thermal decomposition of 138 kJ/mol and 94 kJ/mol, respectively	663:768	This was in accordance with the estimated activation energies of their thermal decomposition of 138 kJ/mol and 94 kJ/mol, respectively.
24299772	2	11	theme	electrospray	450:461	arg1	spectrometry					468:479	electrospray mass spectrometry	450:479	electrospray mass spectrometry	450:479	The resultant products were analysed according to the sugars and linkage composition and also by electrospray mass spectrometry.
24299772	6	12	theme	reducing	1108:1115	arg1	end					1117:1119	the reducing end	1104:1119	the reducing end	1104:1119	Depolymerisation and formation of anhydrohexose residues at the reducing end and mannose-glucose isomerisation were also observed.
24299772	6	13	theme	anhydrohexose	1078:1090	arg1	residues					1092:1099	anhydrohexose residues	1078:1099	anhydrohexose residues at the reducing end	1078:1119	Depolymerisation and formation of anhydrohexose residues at the reducing end and mannose-glucose isomerisation were also observed.
24299772	1	14	theme	long	261:264	arg1	exposures					282:290	long term isothermal exposures	261:290	long term isothermal exposures	261:290	In order to better understand the thermal stability of spent coffee grounds (SCG) galactomannans and arabinogalactans and the reactions that can occur upon roasting, long term isothermal exposures, up to 3h, were performed at 160, 180, 200, 220, and 240 °C.
24299772	5	15	theme	terminally-linked	974:990	arg1	2,3-					880:883	2,3-	880:883	2,3-	880:883	The roasting of galactomannans promoted the formation of new glycosidic linkages, with occurrence of 2-, 6-, 2,3-, 2,6-, 3,6-, 2,3,6-, 3,4,6-linked mannose residues, 3,4,6-linked galactose residues, and terminally-linked glucose residues, observed by methylation analysis.
24299772	5	15	theme	terminally-linked	974:990	arg1	residues					1000:1007	terminally-linked glucose residues	974:1007	terminally-linked glucose residues	974:1007	The roasting of galactomannans promoted the formation of new glycosidic linkages, with occurrence of 2-, 6-, 2,3-, 2,6-, 3,6-, 2,3,6-, 3,4,6-linked mannose residues, 3,4,6-linked galactose residues, and terminally-linked glucose residues, observed by methylation analysis.
24299772	4	16	theme	kJ/mol	749:754	arg1	energies					688:695	the estimated activation energies	663:695	the estimated activation energies of their thermal decomposition of 138 kJ/mol and 94 kJ/mol, respectively	663:768	This was in accordance with the estimated activation energies of their thermal decomposition of 138 kJ/mol and 94 kJ/mol, respectively.
24299772	1	17	dep	understand	114:123	arg1	to					104:105	to	104:105	to	104:105	In order to better understand the thermal stability of spent coffee grounds (SCG) galactomannans and arabinogalactans and the reactions that can occur upon roasting, long term isothermal exposures, up to 3h, were performed at 160, 180, 200, 220, and 240 °C.
24299772	6	18	from	formation	1065:1073	arg1	end					1117:1119	the reducing end	1104:1119	the reducing end	1104:1119	Depolymerisation and formation of anhydrohexose residues at the reducing end and mannose-glucose isomerisation were also observed.
24299772	5	19	theme	glucose	992:998	arg1	2,3-					880:883	2,3-	880:883	2,3-	880:883	The roasting of galactomannans promoted the formation of new glycosidic linkages, with occurrence of 2-, 6-, 2,3-, 2,6-, 3,6-, 2,3,6-, 3,4,6-linked mannose residues, 3,4,6-linked galactose residues, and terminally-linked glucose residues, observed by methylation analysis.
24299772	5	19	theme	glucose	992:998	arg1	residues					1000:1007	terminally-linked glucose residues	974:1007	terminally-linked glucose residues	974:1007	The roasting of galactomannans promoted the formation of new glycosidic linkages, with occurrence of 2-, 6-, 2,3-, 2,6-, 3,6-, 2,3,6-, 3,4,6-linked mannose residues, 3,4,6-linked galactose residues, and terminally-linked glucose residues, observed by methylation analysis.
24299772	4	20	theme	activation	677:686	arg1	energies					688:695	the estimated activation energies	663:695	the estimated activation energies of their thermal decomposition of 138 kJ/mol and 94 kJ/mol, respectively	663:768	This was in accordance with the estimated activation energies of their thermal decomposition of 138 kJ/mol and 94 kJ/mol, respectively.
24299772	1	21	theme	SCG	172:174	arg1	galactomannans					177:190	spent coffee grounds (SCG) galactomannans	150:190	spent coffee grounds (SCG) galactomannans	150:190	In order to better understand the thermal stability of spent coffee grounds (SCG) galactomannans and arabinogalactans and the reactions that can occur upon roasting, long term isothermal exposures, up to 3h, were performed at 160, 180, 200, 220, and 240 °C.
24299772	5	22	link	terminally-linked	974:990	arg1	2,3-					880:883	2,3-	880:883	2,3-	880:883	The roasting of galactomannans promoted the formation of new glycosidic linkages, with occurrence of 2-, 6-, 2,3-, 2,6-, 3,6-, 2,3,6-, 3,4,6-linked mannose residues, 3,4,6-linked galactose residues, and terminally-linked glucose residues, observed by methylation analysis.
24299772	5	22	link	terminally-linked	974:990	arg1	residues					1000:1007	terminally-linked glucose residues	974:1007	terminally-linked glucose residues	974:1007	The roasting of galactomannans promoted the formation of new glycosidic linkages, with occurrence of 2-, 6-, 2,3-, 2,6-, 3,6-, 2,3,6-, 3,4,6-linked mannose residues, 3,4,6-linked galactose residues, and terminally-linked glucose residues, observed by methylation analysis.
24299772	0	23	theme	Thermal	0:6	arg1	stability					8:16	Thermal stability	0:16	Thermal stability of spent coffee	0:32	Thermal stability of spent coffee ground polysaccharides: galactomannans and arabinogalactans.
24299772	1	24	theme	term	266:269	arg1	exposures					282:290	long term isothermal exposures	261:290	long term isothermal exposures	261:290	In order to better understand the thermal stability of spent coffee grounds (SCG) galactomannans and arabinogalactans and the reactions that can occur upon roasting, long term isothermal exposures, up to 3h, were performed at 160, 180, 200, 220, and 240 °C.
24299772	5	25	theme	3,4,6-linked	906:917	arg1	residues					927:934	3,4,6-linked mannose residues	906:934	3,4,6-linked mannose residues	906:934	The roasting of galactomannans promoted the formation of new glycosidic linkages, with occurrence of 2-, 6-, 2,3-, 2,6-, 3,6-, 2,3,6-, 3,4,6-linked mannose residues, 3,4,6-linked galactose residues, and terminally-linked glucose residues, observed by methylation analysis.
24299772	5	25	theme	3,4,6-linked	906:917	arg1	2,3-					880:883	2,3-	880:883	2,3-	880:883	The roasting of galactomannans promoted the formation of new glycosidic linkages, with occurrence of 2-, 6-, 2,3-, 2,6-, 3,6-, 2,3,6-, 3,4,6-linked mannose residues, 3,4,6-linked galactose residues, and terminally-linked glucose residues, observed by methylation analysis.
24299772	1	26	theme	galactomannans	177:190	arg1	reactions					221:229	the reactions	217:229	the reactions that can occur upon roasting, long term isothermal exposures, up to 3h, were performed at 160, 180, 200, 220, and 240 °C	217:350	In order to better understand the thermal stability of spent coffee grounds (SCG) galactomannans and arabinogalactans and the reactions that can occur upon roasting, long term isothermal exposures, up to 3h, were performed at 160, 180, 200, 220, and 240 °C.
24299772	1	26	theme	galactomannans	177:190	arg1	stability					137:145	the thermal stability	125:145	the thermal stability of spent coffee grounds (SCG) galactomannans and arabinogalactans	125:211	In order to better understand the thermal stability of spent coffee grounds (SCG) galactomannans and arabinogalactans and the reactions that can occur upon roasting, long term isothermal exposures, up to 3h, were performed at 160, 180, 200, 220, and 240 °C.
24299772	0	27	theme	spent	21:25	arg1	coffee					27:32	spent coffee	21:32	spent coffee	21:32	Thermal stability of spent coffee ground polysaccharides: galactomannans and arabinogalactans.
24299772	1	28	theme	isothermal	271:280	arg1	exposures					282:290	long term isothermal exposures	261:290	long term isothermal exposures	261:290	In order to better understand the thermal stability of spent coffee grounds (SCG) galactomannans and arabinogalactans and the reactions that can occur upon roasting, long term isothermal exposures, up to 3h, were performed at 160, 180, 200, 220, and 240 °C.
24299772	4	29	theme	kJ/mol	735:740	arg1	decomposition					714:726	their thermal decomposition	700:726	their thermal decomposition of 138 kJ/mol	700:740	This was in accordance with the estimated activation energies of their thermal decomposition of 138 kJ/mol and 94 kJ/mol, respectively.
24299772	4	29	theme	kJ/mol	735:740	arg1	kJ/mol					749:754	94 kJ/mol	746:754	94 kJ/mol	746:754	This was in accordance with the estimated activation energies of their thermal decomposition of 138 kJ/mol and 94 kJ/mol, respectively.
24299772	2	30	theme	resultant	357:365	arg1	products					367:374	The resultant products	353:374	The resultant products	353:374	The resultant products were analysed according to the sugars and linkage composition and also by electrospray mass spectrometry.
24299772	6	31	theme	residues	1092:1099	arg1	Depolymerisation					1044:1059	Depolymerisation	1044:1059	Depolymerisation	1044:1059	Depolymerisation and formation of anhydrohexose residues at the reducing end and mannose-glucose isomerisation were also observed.
24299772	6	31	theme	residues	1092:1099	arg1	isomerisation					1141:1153	mannose-glucose isomerisation	1125:1153	mannose-glucose isomerisation	1125:1153	Depolymerisation and formation of anhydrohexose residues at the reducing end and mannose-glucose isomerisation were also observed.
24299772	6	31	theme	residues	1092:1099	arg1	formation					1065:1073	formation	1065:1073	formation	1065:1073	Depolymerisation and formation of anhydrohexose residues at the reducing end and mannose-glucose isomerisation were also observed.
24299772	5	32	theme	3,4,6-linked	937:948	arg1	2,3-					880:883	2,3-	880:883	2,3-	880:883	The roasting of galactomannans promoted the formation of new glycosidic linkages, with occurrence of 2-, 6-, 2,3-, 2,6-, 3,6-, 2,3,6-, 3,4,6-linked mannose residues, 3,4,6-linked galactose residues, and terminally-linked glucose residues, observed by methylation analysis.
24299772	5	32	theme	3,4,6-linked	937:948	arg1	residues					960:967	3,4,6-linked galactose residues	937:967	3,4,6-linked galactose residues	937:967	The roasting of galactomannans promoted the formation of new glycosidic linkages, with occurrence of 2-, 6-, 2,3-, 2,6-, 3,6-, 2,3,6-, 3,4,6-linked mannose residues, 3,4,6-linked galactose residues, and terminally-linked glucose residues, observed by methylation analysis.
24299772	3	33	theme	loss	505:508	arg1	mass					510:513	loss mass	505:513	loss mass at T ≤ 200 °C during exposures up to 3h	505:553	Galactomannans did not loss mass at T ≤ 200 °C during exposures up to 3h whereas the arabinogalactans showed that thermal stability only for T ≤ 180 °C.
24299772	6	34	from	Depolymerisation	1044:1059	arg1	end					1117:1119	the reducing end	1104:1119	the reducing end	1104:1119	Depolymerisation and formation of anhydrohexose residues at the reducing end and mannose-glucose isomerisation were also observed.
24299772	1	35	theme	arabinogalactans	196:211	arg1	reactions					221:229	the reactions	217:229	the reactions that can occur upon roasting, long term isothermal exposures, up to 3h, were performed at 160, 180, 200, 220, and 240 °C	217:350	In order to better understand the thermal stability of spent coffee grounds (SCG) galactomannans and arabinogalactans and the reactions that can occur upon roasting, long term isothermal exposures, up to 3h, were performed at 160, 180, 200, 220, and 240 °C.
24299772	1	35	theme	arabinogalactans	196:211	arg1	stability					137:145	the thermal stability	125:145	the thermal stability of spent coffee grounds (SCG) galactomannans and arabinogalactans	125:211	In order to better understand the thermal stability of spent coffee grounds (SCG) galactomannans and arabinogalactans and the reactions that can occur upon roasting, long term isothermal exposures, up to 3h, were performed at 160, 180, 200, 220, and 240 °C.
24299772	5	36	theme	galactose	950:958	arg1	2,3-					880:883	2,3-	880:883	2,3-	880:883	The roasting of galactomannans promoted the formation of new glycosidic linkages, with occurrence of 2-, 6-, 2,3-, 2,6-, 3,6-, 2,3,6-, 3,4,6-linked mannose residues, 3,4,6-linked galactose residues, and terminally-linked glucose residues, observed by methylation analysis.
24299772	5	36	theme	galactose	950:958	arg1	residues					960:967	3,4,6-linked galactose residues	937:967	3,4,6-linked galactose residues	937:967	The roasting of galactomannans promoted the formation of new glycosidic linkages, with occurrence of 2-, 6-, 2,3-, 2,6-, 3,6-, 2,3,6-, 3,4,6-linked mannose residues, 3,4,6-linked galactose residues, and terminally-linked glucose residues, observed by methylation analysis.
24299772	3	37	from	°C	526:527	arg1	mass					510:513	loss mass	505:513	loss mass at T ≤ 200 °C during exposures up to 3h	505:553	Galactomannans did not loss mass at T ≤ 200 °C during exposures up to 3h whereas the arabinogalactans showed that thermal stability only for T ≤ 180 °C.
24299772	0	38	theme	coffee	27:32	arg1	stability					8:16	Thermal stability	0:16	Thermal stability of spent coffee	0:32	Thermal stability of spent coffee ground polysaccharides: galactomannans and arabinogalactans.
24299772	0	39	dep	polysaccharides	41:55	arg1	arabinogalactans					77:92	arabinogalactans	77:92	arabinogalactans	77:92	Thermal stability of spent coffee ground polysaccharides: galactomannans and arabinogalactans.
24299772	0	39	dep	polysaccharides	41:55	arg1	galactomannans					58:71	galactomannans	58:71	galactomannans	58:71	Thermal stability of spent coffee ground polysaccharides: galactomannans and arabinogalactans.
24299772	7	40	theme	alkali	1256:1261	arg1	extraction					1263:1272	alkali extraction	1256:1272	alkali extraction	1256:1272	The roasting of galactomannans at 200 °C promoted their solubility in water upon alkali extraction and neutralisation.
24299772	3	41	theme	T	518:518	arg1	°C					526:527	T ≤ 200 °C	518:527	T ≤ 200 °C	518:527	Galactomannans did not loss mass at T ≤ 200 °C during exposures up to 3h whereas the arabinogalactans showed that thermal stability only for T ≤ 180 °C.
24299772	7	42	from	°C	1213:1214	arg1	roasting					1179:1186	The roasting	1175:1186	The roasting of galactomannans at 200 °C	1175:1214	The roasting of galactomannans at 200 °C promoted their solubility in water upon alkali extraction and neutralisation.
24299772	7	43	theme	galactomannans	1191:1204	arg1	roasting					1179:1186	The roasting	1175:1186	The roasting of galactomannans at 200 °C	1175:1214	The roasting of galactomannans at 200 °C promoted their solubility in water upon alkali extraction and neutralisation.
24299772	3	44	theme	thermal	596:602	arg1	stability					604:612	that thermal stability	591:612	that thermal stability	591:612	Galactomannans did not loss mass at T ≤ 200 °C during exposures up to 3h whereas the arabinogalactans showed that thermal stability only for T ≤ 180 °C.
24299772	3	45	theme	≤	520:520	arg1	°C					526:527	T ≤ 200 °C	518:527	T ≤ 200 °C	518:527	Galactomannans did not loss mass at T ≤ 200 °C during exposures up to 3h whereas the arabinogalactans showed that thermal stability only for T ≤ 180 °C.
24299772	5	46	theme	new	828:830	arg1	linkages					843:850	new glycosidic linkages	828:850	new glycosidic linkages	828:850	The roasting of galactomannans promoted the formation of new glycosidic linkages, with occurrence of 2-, 6-, 2,3-, 2,6-, 3,6-, 2,3,6-, 3,4,6-linked mannose residues, 3,4,6-linked galactose residues, and terminally-linked glucose residues, observed by methylation analysis.
24299772	5	47	theme	mannose	919:925	arg1	residues					927:934	3,4,6-linked mannose residues	906:934	3,4,6-linked mannose residues	906:934	The roasting of galactomannans promoted the formation of new glycosidic linkages, with occurrence of 2-, 6-, 2,3-, 2,6-, 3,6-, 2,3,6-, 3,4,6-linked mannose residues, 3,4,6-linked galactose residues, and terminally-linked glucose residues, observed by methylation analysis.
24299772	5	47	theme	mannose	919:925	arg1	2,3-					880:883	2,3-	880:883	2,3-	880:883	The roasting of galactomannans promoted the formation of new glycosidic linkages, with occurrence of 2-, 6-, 2,3-, 2,6-, 3,6-, 2,3,6-, 3,4,6-linked mannose residues, 3,4,6-linked galactose residues, and terminally-linked glucose residues, observed by methylation analysis.
24299772	1	48	theme	thermal	129:135	arg1	stability					137:145	the thermal stability	125:145	the thermal stability of spent coffee grounds (SCG) galactomannans and arabinogalactans	125:211	In order to better understand the thermal stability of spent coffee grounds (SCG) galactomannans and arabinogalactans and the reactions that can occur upon roasting, long term isothermal exposures, up to 3h, were performed at 160, 180, 200, 220, and 240 °C.
24299772	3	49	dep	3h	552:553	arg1	up					546:547	up	546:547	up	546:547	Galactomannans did not loss mass at T ≤ 200 °C during exposures up to 3h whereas the arabinogalactans showed that thermal stability only for T ≤ 180 °C.
24299772	5	50	theme	2-	872:873	arg1	occurrence					858:867	occurrence	858:867	occurrence of 2-, 6-, 2,3-, 2,6-, 3,6-, 2,3,6-, 3,4,6-linked mannose residues, 3,4,6-linked galactose residues, and terminally-linked glucose residues, observed by methylation analysis	858:1041	The roasting of galactomannans promoted the formation of new glycosidic linkages, with occurrence of 2-, 6-, 2,3-, 2,6-, 3,6-, 2,3,6-, 3,4,6-linked mannose residues, 3,4,6-linked galactose residues, and terminally-linked glucose residues, observed by methylation analysis.
24299772	7	51	from	solubility	1231:1240	arg1	water					1245:1249	water	1245:1249	water	1245:1249	The roasting of galactomannans at 200 °C promoted their solubility in water upon alkali extraction and neutralisation.
24299772	5	52	theme	linkages	843:850	arg1	formation					815:823	the formation	811:823	the formation of new glycosidic linkages	811:850	The roasting of galactomannans promoted the formation of new glycosidic linkages, with occurrence of 2-, 6-, 2,3-, 2,6-, 3,6-, 2,3,6-, 3,4,6-linked mannose residues, 3,4,6-linked galactose residues, and terminally-linked glucose residues, observed by methylation analysis.
24299772	5	53	link	3,4,6-linked	906:917	arg1	residues					927:934	3,4,6-linked mannose residues	906:934	3,4,6-linked mannose residues	906:934	The roasting of galactomannans promoted the formation of new glycosidic linkages, with occurrence of 2-, 6-, 2,3-, 2,6-, 3,6-, 2,3,6-, 3,4,6-linked mannose residues, 3,4,6-linked galactose residues, and terminally-linked glucose residues, observed by methylation analysis.
24299772	5	53	link	3,4,6-linked	906:917	arg1	2,3-					880:883	2,3-	880:883	2,3-	880:883	The roasting of galactomannans promoted the formation of new glycosidic linkages, with occurrence of 2-, 6-, 2,3-, 2,6-, 3,6-, 2,3,6-, 3,4,6-linked mannose residues, 3,4,6-linked galactose residues, and terminally-linked glucose residues, observed by methylation analysis.
24299772	5	54	theme	glycosidic	832:841	arg1	linkages					843:850	new glycosidic linkages	828:850	new glycosidic linkages	828:850	The roasting of galactomannans promoted the formation of new glycosidic linkages, with occurrence of 2-, 6-, 2,3-, 2,6-, 3,6-, 2,3,6-, 3,4,6-linked mannose residues, 3,4,6-linked galactose residues, and terminally-linked glucose residues, observed by methylation analysis.
24299772	4	55	theme	decomposition	714:726	arg1	energies					688:695	the estimated activation energies	663:695	the estimated activation energies of their thermal decomposition of 138 kJ/mol and 94 kJ/mol, respectively	663:768	This was in accordance with the estimated activation energies of their thermal decomposition of 138 kJ/mol and 94 kJ/mol, respectively.
24299772	5	56	theme	galactomannans	787:800	arg1	roasting					775:782	The roasting	771:782	The roasting of galactomannans	771:800	The roasting of galactomannans promoted the formation of new glycosidic linkages, with occurrence of 2-, 6-, 2,3-, 2,6-, 3,6-, 2,3,6-, 3,4,6-linked mannose residues, 3,4,6-linked galactose residues, and terminally-linked glucose residues, observed by methylation analysis.
24299772	6	57	from	isomerisation	1141:1153	arg1	end					1117:1119	the reducing end	1104:1119	the reducing end	1104:1119	Depolymerisation and formation of anhydrohexose residues at the reducing end and mannose-glucose isomerisation were also observed.
24299772	3	58	theme	T	623:623	arg1	°C					631:632	T ≤ 180 °C	623:632	T ≤ 180 °C	623:632	Galactomannans did not loss mass at T ≤ 200 °C during exposures up to 3h whereas the arabinogalactans showed that thermal stability only for T ≤ 180 °C.
24299772	4	59	theme	thermal	706:712	arg1	decomposition					714:726	their thermal decomposition	700:726	their thermal decomposition of 138 kJ/mol	700:740	This was in accordance with the estimated activation energies of their thermal decomposition of 138 kJ/mol and 94 kJ/mol, respectively.
24299772	1	60	theme	spent	150:154	arg1	galactomannans					177:190	spent coffee grounds (SCG) galactomannans	150:190	spent coffee grounds (SCG) galactomannans	150:190	In order to better understand the thermal stability of spent coffee grounds (SCG) galactomannans and arabinogalactans and the reactions that can occur upon roasting, long term isothermal exposures, up to 3h, were performed at 160, 180, 200, 220, and 240 °C.
28365540	0	0	theme	composite	77:85	arg1	hydrogels					87:95	composite hydrogels	77:95	composite hydrogels	77:95	Unusual effect evidenced at the investigations of the mechanical behavior of composite hydrogels under cyclic compression.
28365540	1	1	theme	speed	352:356	arg1	variation					323:331	the broad variation	313:331	the broad variation of the deformation speed	313:356	An unusual type of mechanical behavior was registered while studying the swollen hydrogel compositions "cellulose-polyacrylamide" in the conditions of multiple cyclic compression tests with the broad variation of the deformation speed.
28365540	4	2	theme	deformation	899:909	arg1	speed					886:890	the speed	882:890	the speed of the deformation	882:909	But while increasing the speed of the deformation the tendency to the progressive approach of the compression and decompression curves to each other is clearly seen.
28365540	1	3	theme	behavior	153:160	arg1	type					134:137	An unusual type	123:137	An unusual type of mechanical behavior	123:160	An unusual type of mechanical behavior was registered while studying the swollen hydrogel compositions "cellulose-polyacrylamide" in the conditions of multiple cyclic compression tests with the broad variation of the deformation speed.
28365540	2	4	theme	curves	508:513	arg1	parts					474:478	compression and decompression parts	444:478	compression and decompression parts of the cyclic stress-strain curves	444:513	While increasing the deformation rate the clearly seen inversion of the positions of compression and decompression parts of the cyclic stress-strain curves was detected.
28365540	5	5	theme	speed	1124:1128	arg1	value					1099:1103	some value	1094:1103	some value of the deformation speed	1094:1128	This effect results in the full disappearance of the hysteresis at some value of the deformation speed: the decompression curve coincides with the compression curve.
28365540	6	6	theme	speed	1244:1248	arg1	increase					1216:1223	the further increase	1204:1223	the further increase of the deformation speed	1204:1248	Along with the further increase of the deformation speed the hysteresis appears again but the curve corresponding to compression process is situated inferior the curve corresponding to decompression: the "inversion" of the hysteresis was detected.
28365540	3	7	dep	speed	609:613	arg1	%					629:629	about 100-200%	616:629	about 100-200% of the initial sample's height	616:660	While carrying out the cyclic compression tests with relatively low deformation speed (about 100-200% of the initial sample's height per minute) the well defined hysteresis of the stress-strain curve can de seen and in these conditions the decompression part of the curve is situated inferior the part corresponding to compression.
28365540	7	8	theme	process	1471:1477	arg1	character					1453:1461	The precise character	1441:1461	The precise character of this process	1441:1477	The precise character of this process depends upon the stiffness of the hydrogel under study.
28365540	8	9	theme	convincing	1550:1559	arg1	explanation					1561:1571	the convincing explanation	1546:1571	the convincing explanation o	1546:1573	Up to date the convincing explanation o this effect can not be put forward.
28365540	1	10	theme	multiple	274:281	arg1	tests					302:306	multiple cyclic compression tests	274:306	multiple cyclic compression tests with the broad variation of the deformation speed	274:356	An unusual type of mechanical behavior was registered while studying the swollen hydrogel compositions "cellulose-polyacrylamide" in the conditions of multiple cyclic compression tests with the broad variation of the deformation speed.
28365540	0	11	theme	hydrogels	87:95	arg1	behavior					65:72	the mechanical behavior	50:72	the mechanical behavior of composite hydrogels under cyclic compression	50:120	Unusual effect evidenced at the investigations of the mechanical behavior of composite hydrogels under cyclic compression.
28365540	1	12	theme	cyclic	283:288	arg1	tests					302:306	multiple cyclic compression tests	274:306	multiple cyclic compression tests with the broad variation of the deformation speed	274:356	An unusual type of mechanical behavior was registered while studying the swollen hydrogel compositions "cellulose-polyacrylamide" in the conditions of multiple cyclic compression tests with the broad variation of the deformation speed.
28365540	4	13	theme	compression	959:969	arg1	curves					989:994	the compression and decompression curves	955:994	the compression and decompression curves	955:994	But while increasing the speed of the deformation the tendency to the progressive approach of the compression and decompression curves to each other is clearly seen.
28365540	2	14	theme	positions	431:439	arg1	inversion					414:422	the clearly seen inversion	397:422	the clearly seen inversion of the positions of compression and decompression parts of the cyclic stress-strain curves	397:513	While increasing the deformation rate the clearly seen inversion of the positions of compression and decompression parts of the cyclic stress-strain curves was detected.
28365540	3	15	theme	cyclic	552:557	arg1	tests					571:575	the cyclic compression tests	548:575	the cyclic compression tests with relatively low deformation speed (about 100-200% of the initial sample's height per minute)	548:672	While carrying out the cyclic compression tests with relatively low deformation speed (about 100-200% of the initial sample's height per minute) the well defined hysteresis of the stress-strain curve can de seen and in these conditions the decompression part of the curve is situated inferior the part corresponding to compression.
28365540	0	16	theme	cyclic	103:108	arg1	compression					110:120	cyclic compression	103:120	cyclic compression	103:120	Unusual effect evidenced at the investigations of the mechanical behavior of composite hydrogels under cyclic compression.
28365540	3	17	theme	curve	723:727	arg1	hysteresis					691:700	the well defined hysteresis	674:700	the well defined hysteresis of the stress-strain curve	674:727	While carrying out the cyclic compression tests with relatively low deformation speed (about 100-200% of the initial sample's height per minute) the well defined hysteresis of the stress-strain curve can de seen and in these conditions the decompression part of the curve is situated inferior the part corresponding to compression.
28365540	0	18	theme	Unusual	0:6	arg1	effect					8:13	Unusual effect	0:13	Unusual effect	0:13	Unusual effect evidenced at the investigations of the mechanical behavior of composite hydrogels under cyclic compression.
28365540	2	19	theme	seen	409:412	arg1	inversion					414:422	the clearly seen inversion	397:422	the clearly seen inversion of the positions of compression and decompression parts of the cyclic stress-strain curves	397:513	While increasing the deformation rate the clearly seen inversion of the positions of compression and decompression parts of the cyclic stress-strain curves was detected.
28365540	2	20	theme	parts	474:478	arg1	positions					431:439	the positions	427:439	the positions of compression and decompression parts of the cyclic stress-strain curves	427:513	While increasing the deformation rate the clearly seen inversion of the positions of compression and decompression parts of the cyclic stress-strain curves was detected.
28365540	4	21	theme	curves	989:994	arg1	approach					943:950	the progressive approach	927:950	the progressive approach of the compression and decompression curves to each other	927:1008	But while increasing the speed of the deformation the tendency to the progressive approach of the compression and decompression curves to each other is clearly seen.
28365540	5	22	theme	compression	1174:1184	arg1	curve					1186:1190	the compression curve	1170:1190	the compression curve	1170:1190	This effect results in the full disappearance of the hysteresis at some value of the deformation speed: the decompression curve coincides with the compression curve.
28365540	4	23	theme	decompression	975:987	arg1	curves					989:994	the compression and decompression curves	955:994	the compression and decompression curves	955:994	But while increasing the speed of the deformation the tendency to the progressive approach of the compression and decompression curves to each other is clearly seen.
28365540	3	24	theme	deformation	597:607	arg1	speed					609:613	relatively low deformation speed	582:613	relatively low deformation speed (about 100-200% of the initial sample's height per minute)	582:672	While carrying out the cyclic compression tests with relatively low deformation speed (about 100-200% of the initial sample's height per minute) the well defined hysteresis of the stress-strain curve can de seen and in these conditions the decompression part of the curve is situated inferior the part corresponding to compression.
28365540	2	25	theme	decompression	460:472	arg1	parts					474:478	compression and decompression parts	444:478	compression and decompression parts of the cyclic stress-strain curves	444:513	While increasing the deformation rate the clearly seen inversion of the positions of compression and decompression parts of the cyclic stress-strain curves was detected.
28365540	1	26	theme	compression	290:300	arg1	tests					302:306	multiple cyclic compression tests	274:306	multiple cyclic compression tests with the broad variation of the deformation speed	274:356	An unusual type of mechanical behavior was registered while studying the swollen hydrogel compositions "cellulose-polyacrylamide" in the conditions of multiple cyclic compression tests with the broad variation of the deformation speed.
28365540	6	27	theme	deformation	1232:1242	arg1	speed					1244:1248	the deformation speed	1228:1248	the deformation speed	1228:1248	Along with the further increase of the deformation speed the hysteresis appears again but the curve corresponding to compression process is situated inferior the curve corresponding to decompression: the "inversion" of the hysteresis was detected.
28365540	3	28	theme	initial	638:644	arg1	height					655:660	the initial sample's height	634:660	the initial sample's height	634:660	While carrying out the cyclic compression tests with relatively low deformation speed (about 100-200% of the initial sample's height per minute) the well defined hysteresis of the stress-strain curve can de seen and in these conditions the decompression part of the curve is situated inferior the part corresponding to compression.
28365540	1	29	theme	tests	302:306	arg1	conditions					260:269	the conditions	256:269	the conditions of multiple cyclic compression tests with the broad variation of the deformation speed	256:356	An unusual type of mechanical behavior was registered while studying the swollen hydrogel compositions "cellulose-polyacrylamide" in the conditions of multiple cyclic compression tests with the broad variation of the deformation speed.
28365540	3	30	from	inferior	813:820	arg1	conditions					754:763	these conditions	748:763	these conditions	748:763	While carrying out the cyclic compression tests with relatively low deformation speed (about 100-200% of the initial sample's height per minute) the well defined hysteresis of the stress-strain curve can de seen and in these conditions the decompression part of the curve is situated inferior the part corresponding to compression.
28365540	1	31	with	tests	302:306	arg1	variation					323:331	the broad variation	313:331	the broad variation of the deformation speed	313:356	An unusual type of mechanical behavior was registered while studying the swollen hydrogel compositions "cellulose-polyacrylamide" in the conditions of multiple cyclic compression tests with the broad variation of the deformation speed.
28365540	1	32	theme	swollen	196:202	arg1	compositions					213:224	the swollen hydrogel compositions	192:224	the swollen hydrogel compositions "cellulose-polyacrylamide"	192:251	An unusual type of mechanical behavior was registered while studying the swollen hydrogel compositions "cellulose-polyacrylamide" in the conditions of multiple cyclic compression tests with the broad variation of the deformation speed.
28365540	2	33	theme	compression	444:454	arg1	parts					474:478	compression and decompression parts	444:478	compression and decompression parts of the cyclic stress-strain curves	444:513	While increasing the deformation rate the clearly seen inversion of the positions of compression and decompression parts of the cyclic stress-strain curves was detected.
28365540	5	34	theme	deformation	1112:1122	arg1	speed					1124:1128	the deformation speed	1108:1128	the deformation speed	1108:1128	This effect results in the full disappearance of the hysteresis at some value of the deformation speed: the decompression curve coincides with the compression curve.
28365540	6	35	theme	situated	1333:1340	arg1	curve					1287:1291	the curve	1283:1291	the curve corresponding to compression process	1283:1328	Along with the further increase of the deformation speed the hysteresis appears again but the curve corresponding to compression process is situated inferior the curve corresponding to decompression: the "inversion" of the hysteresis was detected.
28365540	6	35	theme	situated	1333:1340	arg1	curve					1355:1359	situated inferior the curve	1333:1359	situated inferior the curve corresponding to decompression	1333:1390	Along with the further increase of the deformation speed the hysteresis appears again but the curve corresponding to compression process is situated inferior the curve corresponding to decompression: the "inversion" of the hysteresis was detected.
28365540	3	36	theme	low	593:595	arg1	speed					609:613	relatively low deformation speed	582:613	relatively low deformation speed (about 100-200% of the initial sample's height per minute)	582:672	While carrying out the cyclic compression tests with relatively low deformation speed (about 100-200% of the initial sample's height per minute) the well defined hysteresis of the stress-strain curve can de seen and in these conditions the decompression part of the curve is situated inferior the part corresponding to compression.
28365540	1	37	theme	hydrogel	204:211	arg1	compositions					213:224	the swollen hydrogel compositions	192:224	the swollen hydrogel compositions "cellulose-polyacrylamide"	192:251	An unusual type of mechanical behavior was registered while studying the swollen hydrogel compositions "cellulose-polyacrylamide" in the conditions of multiple cyclic compression tests with the broad variation of the deformation speed.
28365540	2	38	theme	stress-strain	494:506	arg1	curves					508:513	the cyclic stress-strain curves	483:513	the cyclic stress-strain curves	483:513	While increasing the deformation rate the clearly seen inversion of the positions of compression and decompression parts of the cyclic stress-strain curves was detected.
28365540	4	39	theme	progressive	931:941	arg1	approach					943:950	the progressive approach	927:950	the progressive approach of the compression and decompression curves to each other	927:1008	But while increasing the speed of the deformation the tendency to the progressive approach of the compression and decompression curves to each other is clearly seen.
28365540	2	40	theme	cyclic	487:492	arg1	curves					508:513	the cyclic stress-strain curves	483:513	the cyclic stress-strain curves	483:513	While increasing the deformation rate the clearly seen inversion of the positions of compression and decompression parts of the cyclic stress-strain curves was detected.
28365540	1	41	theme	broad	317:321	arg1	variation					323:331	the broad variation	313:331	the broad variation of the deformation speed	313:356	An unusual type of mechanical behavior was registered while studying the swollen hydrogel compositions "cellulose-polyacrylamide" in the conditions of multiple cyclic compression tests with the broad variation of the deformation speed.
28365540	7	42	theme	hydrogel	1513:1520	arg1	stiffness					1496:1504	the stiffness	1492:1504	the stiffness of the hydrogel under study	1492:1532	The precise character of this process depends upon the stiffness of the hydrogel under study.
28365540	7	43	theme	precise	1445:1451	arg1	character					1453:1461	The precise character	1441:1461	The precise character of this process	1441:1477	The precise character of this process depends upon the stiffness of the hydrogel under study.
28365540	3	44	dep	seen	736:739	arg1	de					733:734	de	733:734	de	733:734	While carrying out the cyclic compression tests with relatively low deformation speed (about 100-200% of the initial sample's height per minute) the well defined hysteresis of the stress-strain curve can de seen and in these conditions the decompression part of the curve is situated inferior the part corresponding to compression.
28365540	3	45	with	tests	571:575	arg1	speed					609:613	relatively low deformation speed	582:613	relatively low deformation speed (about 100-200% of the initial sample's height per minute)	582:672	While carrying out the cyclic compression tests with relatively low deformation speed (about 100-200% of the initial sample's height per minute) the well defined hysteresis of the stress-strain curve can de seen and in these conditions the decompression part of the curve is situated inferior the part corresponding to compression.
28365540	5	46	theme	decompression	1135:1147	arg1	curve					1149:1153	the decompression curve	1131:1153	the decompression curve	1131:1153	This effect results in the full disappearance of the hysteresis at some value of the deformation speed: the decompression curve coincides with the compression curve.
28365540	6	47	theme	compression	1310:1320	arg1	process					1322:1328	compression process	1310:1328	compression process	1310:1328	Along with the further increase of the deformation speed the hysteresis appears again but the curve corresponding to compression process is situated inferior the curve corresponding to decompression: the "inversion" of the hysteresis was detected.
28365540	5	48	theme	hysteresis	1080:1089	arg1	disappearance					1059:1071	the full disappearance	1050:1071	the full disappearance of the hysteresis at some value of the deformation speed	1050:1128	This effect results in the full disappearance of the hysteresis at some value of the deformation speed: the decompression curve coincides with the compression curve.
28365540	6	49	dep	situated	1333:1340	arg1	inferior					1342:1349	inferior	1342:1349	inferior	1342:1349	Along with the further increase of the deformation speed the hysteresis appears again but the curve corresponding to compression process is situated inferior the curve corresponding to decompression: the "inversion" of the hysteresis was detected.
28365540	6	50	theme	inversion	1398:1406	arg1	"					1407:1407	the "inversion"	1393:1407	the "inversion" of the hysteresis	1393:1425	Along with the further increase of the deformation speed the hysteresis appears again but the curve corresponding to compression process is situated inferior the curve corresponding to decompression: the "inversion" of the hysteresis was detected.
28365540	3	51	dep	inferior	813:820	arg1	part					826:829	the part	822:829	the part corresponding to compression	822:858	While carrying out the cyclic compression tests with relatively low deformation speed (about 100-200% of the initial sample's height per minute) the well defined hysteresis of the stress-strain curve can de seen and in these conditions the decompression part of the curve is situated inferior the part corresponding to compression.
28365540	6	52	theme	further	1208:1214	arg1	increase					1216:1223	the further increase	1204:1223	the further increase of the deformation speed	1204:1248	Along with the further increase of the deformation speed the hysteresis appears again but the curve corresponding to compression process is situated inferior the curve corresponding to decompression: the "inversion" of the hysteresis was detected.
28365540	5	53	from	value	1099:1103	arg1	disappearance					1059:1071	the full disappearance	1050:1071	the full disappearance of the hysteresis at some value of the deformation speed	1050:1128	This effect results in the full disappearance of the hysteresis at some value of the deformation speed: the decompression curve coincides with the compression curve.
28365540	3	54	theme	decompression	769:781	arg1	part					783:786	the decompression part	765:786	the decompression part of the curve	765:799	While carrying out the cyclic compression tests with relatively low deformation speed (about 100-200% of the initial sample's height per minute) the well defined hysteresis of the stress-strain curve can de seen and in these conditions the decompression part of the curve is situated inferior the part corresponding to compression.
28365540	3	54	theme	decompression	769:781	arg1	inferior					813:820	situated inferior	804:820	situated inferior	804:820	While carrying out the cyclic compression tests with relatively low deformation speed (about 100-200% of the initial sample's height per minute) the well defined hysteresis of the stress-strain curve can de seen and in these conditions the decompression part of the curve is situated inferior the part corresponding to compression.
28365540	3	55	theme	defined	683:689	arg1	hysteresis					691:700	the well defined hysteresis	674:700	the well defined hysteresis of the stress-strain curve	674:727	While carrying out the cyclic compression tests with relatively low deformation speed (about 100-200% of the initial sample's height per minute) the well defined hysteresis of the stress-strain curve can de seen and in these conditions the decompression part of the curve is situated inferior the part corresponding to compression.
28365540	3	56	theme	situated	804:811	arg1	part					783:786	the decompression part	765:786	the decompression part of the curve	765:799	While carrying out the cyclic compression tests with relatively low deformation speed (about 100-200% of the initial sample's height per minute) the well defined hysteresis of the stress-strain curve can de seen and in these conditions the decompression part of the curve is situated inferior the part corresponding to compression.
28365540	3	56	theme	situated	804:811	arg1	inferior					813:820	situated inferior	804:820	situated inferior	804:820	While carrying out the cyclic compression tests with relatively low deformation speed (about 100-200% of the initial sample's height per minute) the well defined hysteresis of the stress-strain curve can de seen and in these conditions the decompression part of the curve is situated inferior the part corresponding to compression.
28365540	1	57	theme	unusual	126:132	arg1	type					134:137	An unusual type	123:137	An unusual type of mechanical behavior	123:160	An unusual type of mechanical behavior was registered while studying the swollen hydrogel compositions "cellulose-polyacrylamide" in the conditions of multiple cyclic compression tests with the broad variation of the deformation speed.
28365540	3	58	theme	compression	559:569	arg1	tests					571:575	the cyclic compression tests	548:575	the cyclic compression tests with relatively low deformation speed (about 100-200% of the initial sample's height per minute)	548:672	While carrying out the cyclic compression tests with relatively low deformation speed (about 100-200% of the initial sample's height per minute) the well defined hysteresis of the stress-strain curve can de seen and in these conditions the decompression part of the curve is situated inferior the part corresponding to compression.
28365540	2	59	theme	deformation	380:390	arg1	rate					392:395	the deformation rate	376:395	the deformation rate	376:395	While increasing the deformation rate the clearly seen inversion of the positions of compression and decompression parts of the cyclic stress-strain curves was detected.
28365540	1	60	theme	"	251:251	arg1	compositions					213:224	the swollen hydrogel compositions	192:224	the swollen hydrogel compositions "cellulose-polyacrylamide"	192:251	An unusual type of mechanical behavior was registered while studying the swollen hydrogel compositions "cellulose-polyacrylamide" in the conditions of multiple cyclic compression tests with the broad variation of the deformation speed.
28365540	0	61	theme	behavior	65:72	arg1	investigations					32:45	the investigations	28:45	the investigations of the mechanical behavior of composite hydrogels under cyclic compression	28:120	Unusual effect evidenced at the investigations of the mechanical behavior of composite hydrogels under cyclic compression.
28365540	5	62	theme	full	1054:1057	arg1	disappearance					1059:1071	the full disappearance	1050:1071	the full disappearance of the hysteresis at some value of the deformation speed	1050:1128	This effect results in the full disappearance of the hysteresis at some value of the deformation speed: the decompression curve coincides with the compression curve.
28365540	8	63	theme	explanation	1561:1571	arg1	o					1573:1573	the convincing explanation o	1546:1573	the convincing explanation o	1546:1573	Up to date the convincing explanation o this effect can not be put forward.
28365540	0	64	theme	mechanical	54:63	arg1	behavior					65:72	the mechanical behavior	50:72	the mechanical behavior of composite hydrogels under cyclic compression	50:120	Unusual effect evidenced at the investigations of the mechanical behavior of composite hydrogels under cyclic compression.
28365540	1	65	theme	deformation	340:350	arg1	speed					352:356	the deformation speed	336:356	the deformation speed	336:356	An unusual type of mechanical behavior was registered while studying the swollen hydrogel compositions "cellulose-polyacrylamide" in the conditions of multiple cyclic compression tests with the broad variation of the deformation speed.
28365540	6	66	theme	hysteresis	1416:1425	arg1	"					1407:1407	the "inversion"	1393:1407	the "inversion" of the hysteresis	1393:1425	Along with the further increase of the deformation speed the hysteresis appears again but the curve corresponding to compression process is situated inferior the curve corresponding to decompression: the "inversion" of the hysteresis was detected.
28365540	3	67	theme	stress-strain	709:721	arg1	curve					723:727	the stress-strain curve	705:727	the stress-strain curve	705:727	While carrying out the cyclic compression tests with relatively low deformation speed (about 100-200% of the initial sample's height per minute) the well defined hysteresis of the stress-strain curve can de seen and in these conditions the decompression part of the curve is situated inferior the part corresponding to compression.
28365540	1	68	theme	mechanical	142:151	arg1	behavior					153:160	mechanical behavior	142:160	mechanical behavior	142:160	An unusual type of mechanical behavior was registered while studying the swollen hydrogel compositions "cellulose-polyacrylamide" in the conditions of multiple cyclic compression tests with the broad variation of the deformation speed.
28365540	3	69	theme	curve	795:799	arg1	part					783:786	the decompression part	765:786	the decompression part of the curve	765:799	While carrying out the cyclic compression tests with relatively low deformation speed (about 100-200% of the initial sample's height per minute) the well defined hysteresis of the stress-strain curve can de seen and in these conditions the decompression part of the curve is situated inferior the part corresponding to compression.
28365540	3	69	theme	curve	795:799	arg1	inferior					813:820	situated inferior	804:820	situated inferior	804:820	While carrying out the cyclic compression tests with relatively low deformation speed (about 100-200% of the initial sample's height per minute) the well defined hysteresis of the stress-strain curve can de seen and in these conditions the decompression part of the curve is situated inferior the part corresponding to compression.
26266984	7	0	theme	classical	1159:1167	arg1	model					1188:1192	the classical alternating-access model	1155:1192	the classical alternating-access model applied to other transporters	1155:1222	The proposed mechanism is distinct from the classical alternating-access model applied to other transporters.
26266984	2	1	theme	oligosaccharides	351:366	arg1	translocation					321:333	the translocation	317:333	the translocation of lipid-linked oligosaccharides that serve as donors in N-linked protein glycosylation	317:421	A prominent example of a flipping reaction is the translocation of lipid-linked oligosaccharides that serve as donors in N-linked protein glycosylation.
26266984	2	1	theme	oligosaccharides	351:366	arg1	example					283:289	A prominent example	271:289	A prominent example of a flipping reaction	271:312	A prominent example of a flipping reaction is the translocation of lipid-linked oligosaccharides that serve as donors in N-linked protein glycosylation.
26266984	4	2	theme	crystal	597:603	arg1	structures					605:614	crystal structures	597:614	crystal structures in distinct states, a newly devised in vitro flipping assay, and in vivo studies	597:695	Here we present a mechanism of PglK-catalysed lipid-linked oligosaccharide flipping based on crystal structures in distinct states, a newly devised in vitro flipping assay, and in vivo studies.
26266984	7	3	theme	alternating-access	1169:1186	arg1	model					1188:1192	the classical alternating-access model	1155:1192	the classical alternating-access model applied to other transporters	1155:1222	The proposed mechanism is distinct from the classical alternating-access model applied to other transporters.
26266984	2	4	theme	lipid-linked	338:349	arg1	oligosaccharides					351:366	lipid-linked oligosaccharides	338:366	lipid-linked oligosaccharides that serve as donors in N-linked protein glycosylation	338:421	A prominent example of a flipping reaction is the translocation of lipid-linked oligosaccharides that serve as donors in N-linked protein glycosylation.
26266984	2	4	theme	lipid-linked	338:349	arg1	donors					382:387	donors	382:387	donors in N-linked protein glycosylation	382:421	A prominent example of a flipping reaction is the translocation of lipid-linked oligosaccharides that serve as donors in N-linked protein glycosylation.
26266984	4	5	link	lipid-linked	550:561	arg1	oligosaccharide					563:577	PglK-catalysed lipid-linked oligosaccharide flipping	535:586	PglK-catalysed lipid-linked oligosaccharide flipping based on crystal structures in distinct states, a newly devised in vitro flipping assay, and in vivo studies	535:695	Here we present a mechanism of PglK-catalysed lipid-linked oligosaccharide flipping based on crystal structures in distinct states, a newly devised in vitro flipping assay, and in vivo studies.
26266984	2	6	theme	reaction	305:312	arg1	translocation					321:333	the translocation	317:333	the translocation of lipid-linked oligosaccharides that serve as donors in N-linked protein glycosylation	317:421	A prominent example of a flipping reaction is the translocation of lipid-linked oligosaccharides that serve as donors in N-linked protein glycosylation.
26266984	2	6	theme	reaction	305:312	arg1	example					283:289	A prominent example	271:289	A prominent example of a flipping reaction	271:312	A prominent example of a flipping reaction is the translocation of lipid-linked oligosaccharides that serve as donors in N-linked protein glycosylation.
26266984	2	7	theme	prominent	273:281	arg1	translocation					321:333	the translocation	317:333	the translocation of lipid-linked oligosaccharides that serve as donors in N-linked protein glycosylation	317:421	A prominent example of a flipping reaction is the translocation of lipid-linked oligosaccharides that serve as donors in N-linked protein glycosylation.
26266984	2	7	theme	prominent	273:281	arg1	example					283:289	A prominent example	271:289	A prominent example of a flipping reaction	271:312	A prominent example of a flipping reaction is the translocation of lipid-linked oligosaccharides that serve as donors in N-linked protein glycosylation.
26266984	3	8	theme	ABC	482:484	arg1	PglK					498:501	the ABC transporter PglK	478:501	the ABC transporter PglK	478:501	In Campylobacter jejuni, this process is catalysed by the ABC transporter PglK.
26266984	2	9	link	lipid-linked	338:349	arg1	oligosaccharides					351:366	lipid-linked oligosaccharides	338:366	lipid-linked oligosaccharides that serve as donors in N-linked protein glycosylation	338:421	A prominent example of a flipping reaction is the translocation of lipid-linked oligosaccharides that serve as donors in N-linked protein glycosylation.
26266984	2	9	link	lipid-linked	338:349	arg1	donors					382:387	donors	382:387	donors in N-linked protein glycosylation	382:421	A prominent example of a flipping reaction is the translocation of lipid-linked oligosaccharides that serve as donors in N-linked protein glycosylation.
26266984	1	10	dep	large	128:132	arg1	polar					135:139	polar	135:139	polar	135:139	The flipping of membrane-embedded lipids containing large, polar head groups is slow and energetically unfavourable, and is therefore catalysed by flippases, the mechanisms of which are unknown.
26266984	2	11	theme	N-linked	392:399	arg1	glycosylation					409:421	N-linked protein glycosylation	392:421	N-linked protein glycosylation	392:421	A prominent example of a flipping reaction is the translocation of lipid-linked oligosaccharides that serve as donors in N-linked protein glycosylation.
26266984	6	12	theme	translocation	917:929	arg1	cavity					931:936	the translocation cavity	913:936	the translocation cavity	913:936	While the pyrophosphate-oligosaccharide head group of lipid-linked oligosaccharides enters the translocation cavity and interacts with positively charged side chains, the lipidic polyprenyl tail binds and activates the transporter but remains exposed to the lipid bilayer during the reaction.
26266984	5	13	theme	outward-facing	773:786	arg1	states					788:793	only outward-facing states	768:793	only outward-facing states	768:793	PglK can adopt inward- and outward-facing conformations in vitro, but only outward-facing states are required for flipping.
26266984	4	14	dep	in	681:682	arg1	vivo					684:687	vivo	684:687	vivo	684:687	Here we present a mechanism of PglK-catalysed lipid-linked oligosaccharide flipping based on crystal structures in distinct states, a newly devised in vitro flipping assay, and in vivo studies.
26266984	3	15	theme	transporter	486:496	arg1	PglK					498:501	the ABC transporter PglK	478:501	the ABC transporter PglK	478:501	In Campylobacter jejuni, this process is catalysed by the ABC transporter PglK.
26266984	4	16	theme	flipping	661:668	arg1	assay					670:674	a newly devised in vitro flipping assay	636:674	a newly devised in vitro flipping assay	636:674	Here we present a mechanism of PglK-catalysed lipid-linked oligosaccharide flipping based on crystal structures in distinct states, a newly devised in vitro flipping assay, and in vivo studies.
26266984	6	17	theme	lipidic	993:999	arg1	tail					1012:1015	the lipidic polyprenyl tail	989:1015	the lipidic polyprenyl tail	989:1015	While the pyrophosphate-oligosaccharide head group of lipid-linked oligosaccharides enters the translocation cavity and interacts with positively charged side chains, the lipidic polyprenyl tail binds and activates the transporter but remains exposed to the lipid bilayer during the reaction.
26266984	7	18	theme	proposed	1119:1126	arg1	distinct					1141:1148	distinct	1141:1148	distinct	1141:1148	The proposed mechanism is distinct from the classical alternating-access model applied to other transporters.
26266984	7	18	theme	proposed	1119:1126	arg1	mechanism					1128:1136	The proposed mechanism	1115:1136	The proposed mechanism	1115:1136	The proposed mechanism is distinct from the classical alternating-access model applied to other transporters.
26266984	0	19	link	lipid-linked	37:48	arg1	flippase					66:73	an active lipid-linked oligosaccharide flippase	27:73	an active lipid-linked oligosaccharide flippase	27:73	Structure and mechanism of an active lipid-linked oligosaccharide flippase.
26266984	1	20	dep	slow	156:159	arg1	flipping					80:87	The flipping	76:87	The flipping of membrane-embedded lipids containing large, polar head groups	76:151	The flipping of membrane-embedded lipids containing large, polar head groups is slow and energetically unfavourable, and is therefore catalysed by flippases, the mechanisms of which are unknown.
26266984	1	20	dep	slow	156:159	arg1	slow					156:159	slow	156:159	slow	156:159	The flipping of membrane-embedded lipids containing large, polar head groups is slow and energetically unfavourable, and is therefore catalysed by flippases, the mechanisms of which are unknown.
26266984	1	20	dep	slow	156:159	arg1	unfavourable					179:190	unfavourable	179:190	unfavourable	179:190	The flipping of membrane-embedded lipids containing large, polar head groups is slow and energetically unfavourable, and is therefore catalysed by flippases, the mechanisms of which are unknown.
26266984	3	21	theme	Campylobacter	427:439	arg1	jejuni					441:446	Campylobacter jejuni	427:446	Campylobacter jejuni	427:446	In Campylobacter jejuni, this process is catalysed by the ABC transporter PglK.
26266984	4	22	theme	oligosaccharide	563:577	arg1	mechanism					522:530	a mechanism	520:530	a mechanism of PglK-catalysed lipid-linked oligosaccharide flipping based on crystal structures in distinct states, a newly devised in vitro flipping assay, and in vivo studies	520:695	Here we present a mechanism of PglK-catalysed lipid-linked oligosaccharide flipping based on crystal structures in distinct states, a newly devised in vitro flipping assay, and in vivo studies.
26266984	1	23	theme	membrane-embedded	92:108	arg1	lipids					110:115	membrane-embedded lipids	92:115	membrane-embedded lipids containing large, polar head groups	92:151	The flipping of membrane-embedded lipids containing large, polar head groups is slow and energetically unfavourable, and is therefore catalysed by flippases, the mechanisms of which are unknown.
26266984	6	24	theme	oligosaccharides	889:904	arg1	group					867:871	the pyrophosphate-oligosaccharide head group	828:871	the pyrophosphate-oligosaccharide head group of lipid-linked oligosaccharides	828:904	While the pyrophosphate-oligosaccharide head group of lipid-linked oligosaccharides enters the translocation cavity and interacts with positively charged side chains, the lipidic polyprenyl tail binds and activates the transporter but remains exposed to the lipid bilayer during the reaction.
26266984	6	24	theme	oligosaccharides	889:904	arg1	oligosaccharides					889:904	lipid-linked oligosaccharides	876:904	lipid-linked oligosaccharides	876:904	While the pyrophosphate-oligosaccharide head group of lipid-linked oligosaccharides enters the translocation cavity and interacts with positively charged side chains, the lipidic polyprenyl tail binds and activates the transporter but remains exposed to the lipid bilayer during the reaction.
26266984	4	25	theme	lipid-linked	550:561	arg1	oligosaccharide					563:577	PglK-catalysed lipid-linked oligosaccharide flipping	535:586	PglK-catalysed lipid-linked oligosaccharide flipping based on crystal structures in distinct states, a newly devised in vitro flipping assay, and in vivo studies	535:695	Here we present a mechanism of PglK-catalysed lipid-linked oligosaccharide flipping based on crystal structures in distinct states, a newly devised in vitro flipping assay, and in vivo studies.
26266984	1	26	theme	lipids	110:115	arg1	flipping					80:87	The flipping	76:87	The flipping of membrane-embedded lipids containing large, polar head groups	76:151	The flipping of membrane-embedded lipids containing large, polar head groups is slow and energetically unfavourable, and is therefore catalysed by flippases, the mechanisms of which are unknown.
26266984	1	26	theme	lipids	110:115	arg1	slow					156:159	slow	156:159	slow	156:159	The flipping of membrane-embedded lipids containing large, polar head groups is slow and energetically unfavourable, and is therefore catalysed by flippases, the mechanisms of which are unknown.
26266984	1	26	theme	lipids	110:115	arg1	unfavourable					179:190	unfavourable	179:190	unfavourable	179:190	The flipping of membrane-embedded lipids containing large, polar head groups is slow and energetically unfavourable, and is therefore catalysed by flippases, the mechanisms of which are unknown.
26266984	4	27	theme	devised	644:650	arg1	assay					670:674	a newly devised in vitro flipping assay	636:674	a newly devised in vitro flipping assay	636:674	Here we present a mechanism of PglK-catalysed lipid-linked oligosaccharide flipping based on crystal structures in distinct states, a newly devised in vitro flipping assay, and in vivo studies.
26266984	2	28	theme	flipping	296:303	arg1	reaction					305:312	a flipping reaction	294:312	a flipping reaction	294:312	A prominent example of a flipping reaction is the translocation of lipid-linked oligosaccharides that serve as donors in N-linked protein glycosylation.
26266984	6	29	theme	polyprenyl	1001:1010	arg1	tail					1012:1015	the lipidic polyprenyl tail	989:1015	the lipidic polyprenyl tail	989:1015	While the pyrophosphate-oligosaccharide head group of lipid-linked oligosaccharides enters the translocation cavity and interacts with positively charged side chains, the lipidic polyprenyl tail binds and activates the transporter but remains exposed to the lipid bilayer during the reaction.
26266984	0	30	theme	active	30:35	arg1	flippase					66:73	an active lipid-linked oligosaccharide flippase	27:73	an active lipid-linked oligosaccharide flippase	27:73	Structure and mechanism of an active lipid-linked oligosaccharide flippase.
26266984	4	31	theme	flipping	579:586	arg1	oligosaccharide					563:577	PglK-catalysed lipid-linked oligosaccharide flipping	535:586	PglK-catalysed lipid-linked oligosaccharide flipping based on crystal structures in distinct states, a newly devised in vitro flipping assay, and in vivo studies	535:695	Here we present a mechanism of PglK-catalysed lipid-linked oligosaccharide flipping based on crystal structures in distinct states, a newly devised in vitro flipping assay, and in vivo studies.
26266984	5	32	theme	outward-facing	725:738	arg1	conformations					740:752	inward- and outward-facing conformations	713:752	inward- and outward-facing conformations	713:752	PglK can adopt inward- and outward-facing conformations in vitro, but only outward-facing states are required for flipping.
26266984	6	33	theme	lipid	1080:1084	arg1	bilayer					1086:1092	the lipid bilayer	1076:1092	the lipid bilayer	1076:1092	While the pyrophosphate-oligosaccharide head group of lipid-linked oligosaccharides enters the translocation cavity and interacts with positively charged side chains, the lipidic polyprenyl tail binds and activates the transporter but remains exposed to the lipid bilayer during the reaction.
26266984	7	34	theme	other	1205:1209	arg1	transporters					1211:1222	other transporters	1205:1222	other transporters	1205:1222	The proposed mechanism is distinct from the classical alternating-access model applied to other transporters.
26266984	1	35	contain	containing	117:126	arg1	lipids					110:115	membrane-embedded lipids	92:115	membrane-embedded lipids containing large, polar head groups	92:151	The flipping of membrane-embedded lipids containing large, polar head groups is slow and energetically unfavourable, and is therefore catalysed by flippases, the mechanisms of which are unknown.
26266984	1	35	contain	containing	117:126	arg2	groups					146:151	large, polar head groups	128:151	large, polar head groups	128:151	The flipping of membrane-embedded lipids containing large, polar head groups is slow and energetically unfavourable, and is therefore catalysed by flippases, the mechanisms of which are unknown.
26266984	5	36	theme	inward-	713:719	arg1	conformations					740:752	inward- and outward-facing conformations	713:752	inward- and outward-facing conformations	713:752	PglK can adopt inward- and outward-facing conformations in vitro, but only outward-facing states are required for flipping.
26266984	2	37	link	N-linked	392:399	arg1	glycosylation					409:421	N-linked protein glycosylation	392:421	N-linked protein glycosylation	392:421	A prominent example of a flipping reaction is the translocation of lipid-linked oligosaccharides that serve as donors in N-linked protein glycosylation.
26266984	4	38	dep	in	652:653	arg1	vitro					655:659	vitro	655:659	vitro	655:659	Here we present a mechanism of PglK-catalysed lipid-linked oligosaccharide flipping based on crystal structures in distinct states, a newly devised in vitro flipping assay, and in vivo studies.
26266984	2	39	from	donors	382:387	arg1	glycosylation					409:421	N-linked protein glycosylation	392:421	N-linked protein glycosylation	392:421	A prominent example of a flipping reaction is the translocation of lipid-linked oligosaccharides that serve as donors in N-linked protein glycosylation.
26266984	2	40	theme	protein	401:407	arg1	glycosylation					409:421	N-linked protein glycosylation	392:421	N-linked protein glycosylation	392:421	A prominent example of a flipping reaction is the translocation of lipid-linked oligosaccharides that serve as donors in N-linked protein glycosylation.
26266984	0	41	theme	oligosaccharide	50:64	arg1	flippase					66:73	an active lipid-linked oligosaccharide flippase	27:73	an active lipid-linked oligosaccharide flippase	27:73	Structure and mechanism of an active lipid-linked oligosaccharide flippase.
26266984	1	42	theme	large	128:132	arg1	groups					146:151	large, polar head groups	128:151	large, polar head groups	128:151	The flipping of membrane-embedded lipids containing large, polar head groups is slow and energetically unfavourable, and is therefore catalysed by flippases, the mechanisms of which are unknown.
26266984	0	43	theme	lipid-linked	37:48	arg1	flippase					66:73	an active lipid-linked oligosaccharide flippase	27:73	an active lipid-linked oligosaccharide flippase	27:73	Structure and mechanism of an active lipid-linked oligosaccharide flippase.
26266984	4	44	theme	PglK-catalysed	535:548	arg1	oligosaccharide					563:577	PglK-catalysed lipid-linked oligosaccharide flipping	535:586	PglK-catalysed lipid-linked oligosaccharide flipping based on crystal structures in distinct states, a newly devised in vitro flipping assay, and in vivo studies	535:695	Here we present a mechanism of PglK-catalysed lipid-linked oligosaccharide flipping based on crystal structures in distinct states, a newly devised in vitro flipping assay, and in vivo studies.
26266984	7	45	from	model	1188:1192	arg1	distinct					1141:1148	distinct	1141:1148	distinct	1141:1148	The proposed mechanism is distinct from the classical alternating-access model applied to other transporters.
26266984	7	45	from	model	1188:1192	arg1	mechanism					1128:1136	The proposed mechanism	1115:1136	The proposed mechanism	1115:1136	The proposed mechanism is distinct from the classical alternating-access model applied to other transporters.
26266984	6	46	theme	lipid-linked	876:887	arg1	oligosaccharides					889:904	lipid-linked oligosaccharides	876:904	lipid-linked oligosaccharides	876:904	While the pyrophosphate-oligosaccharide head group of lipid-linked oligosaccharides enters the translocation cavity and interacts with positively charged side chains, the lipidic polyprenyl tail binds and activates the transporter but remains exposed to the lipid bilayer during the reaction.
26266984	6	47	theme	side	976:979	arg1	chains					981:986	positively charged side chains	957:986	positively charged side chains	957:986	While the pyrophosphate-oligosaccharide head group of lipid-linked oligosaccharides enters the translocation cavity and interacts with positively charged side chains, the lipidic polyprenyl tail binds and activates the transporter but remains exposed to the lipid bilayer during the reaction.
26266984	6	48	theme	head	862:865	arg1	group					867:871	the pyrophosphate-oligosaccharide head group	828:871	the pyrophosphate-oligosaccharide head group of lipid-linked oligosaccharides	828:904	While the pyrophosphate-oligosaccharide head group of lipid-linked oligosaccharides enters the translocation cavity and interacts with positively charged side chains, the lipidic polyprenyl tail binds and activates the transporter but remains exposed to the lipid bilayer during the reaction.
26266984	6	48	theme	head	862:865	arg1	oligosaccharides					889:904	lipid-linked oligosaccharides	876:904	lipid-linked oligosaccharides	876:904	While the pyrophosphate-oligosaccharide head group of lipid-linked oligosaccharides enters the translocation cavity and interacts with positively charged side chains, the lipidic polyprenyl tail binds and activates the transporter but remains exposed to the lipid bilayer during the reaction.
26266984	1	49	theme	flippases	223:231	arg1	mechanisms					238:247	the mechanisms	234:247	the mechanisms	234:247	The flipping of membrane-embedded lipids containing large, polar head groups is slow and energetically unfavourable, and is therefore catalysed by flippases, the mechanisms of which are unknown.
26266984	1	49	theme	flippases	223:231	arg1	unknown					262:268	unknown	262:268	unknown	262:268	The flipping of membrane-embedded lipids containing large, polar head groups is slow and energetically unfavourable, and is therefore catalysed by flippases, the mechanisms of which are unknown.
26266984	6	50	theme	pyrophosphate-oligosaccharide	832:860	arg1	group					867:871	the pyrophosphate-oligosaccharide head group	828:871	the pyrophosphate-oligosaccharide head group of lipid-linked oligosaccharides	828:904	While the pyrophosphate-oligosaccharide head group of lipid-linked oligosaccharides enters the translocation cavity and interacts with positively charged side chains, the lipidic polyprenyl tail binds and activates the transporter but remains exposed to the lipid bilayer during the reaction.
26266984	6	50	theme	pyrophosphate-oligosaccharide	832:860	arg1	oligosaccharides					889:904	lipid-linked oligosaccharides	876:904	lipid-linked oligosaccharides	876:904	While the pyrophosphate-oligosaccharide head group of lipid-linked oligosaccharides enters the translocation cavity and interacts with positively charged side chains, the lipidic polyprenyl tail binds and activates the transporter but remains exposed to the lipid bilayer during the reaction.
26266984	1	51	theme	head	141:144	arg1	groups					146:151	large, polar head groups	128:151	large, polar head groups	128:151	The flipping of membrane-embedded lipids containing large, polar head groups is slow and energetically unfavourable, and is therefore catalysed by flippases, the mechanisms of which are unknown.
26266984	4	52	theme	distinct	619:626	arg1	states					628:633	distinct states	619:633	distinct states	619:633	Here we present a mechanism of PglK-catalysed lipid-linked oligosaccharide flipping based on crystal structures in distinct states, a newly devised in vitro flipping assay, and in vivo studies.
26266984	4	53	theme	in	652:653	arg1	assay					670:674	a newly devised in vitro flipping assay	636:674	a newly devised in vitro flipping assay	636:674	Here we present a mechanism of PglK-catalysed lipid-linked oligosaccharide flipping based on crystal structures in distinct states, a newly devised in vitro flipping assay, and in vivo studies.
26266984	6	54	link	lipid-linked	876:887	arg1	oligosaccharides					889:904	lipid-linked oligosaccharides	876:904	lipid-linked oligosaccharides	876:904	While the pyrophosphate-oligosaccharide head group of lipid-linked oligosaccharides enters the translocation cavity and interacts with positively charged side chains, the lipidic polyprenyl tail binds and activates the transporter but remains exposed to the lipid bilayer during the reaction.
26266984	0	55	theme	flippase	66:73	arg1	mechanism					14:22	mechanism	14:22	mechanism	14:22	Structure and mechanism of an active lipid-linked oligosaccharide flippase.
26266984	0	55	theme	flippase	66:73	arg1	Structure					0:8	Structure	0:8	Structure	0:8	Structure and mechanism of an active lipid-linked oligosaccharide flippase.
26266984	6	56	theme	charged	968:974	arg1	chains					981:986	positively charged side chains	957:986	positively charged side chains	957:986	While the pyrophosphate-oligosaccharide head group of lipid-linked oligosaccharides enters the translocation cavity and interacts with positively charged side chains, the lipidic polyprenyl tail binds and activates the transporter but remains exposed to the lipid bilayer during the reaction.
26266984	4	57	theme	in	681:682	arg1	studies					689:695	in vivo studies	681:695	in vivo studies	681:695	Here we present a mechanism of PglK-catalysed lipid-linked oligosaccharide flipping based on crystal structures in distinct states, a newly devised in vitro flipping assay, and in vivo studies.
26266984	4	58	from	structures	605:614	arg1	studies					689:695	in vivo studies	681:695	in vivo studies	681:695	Here we present a mechanism of PglK-catalysed lipid-linked oligosaccharide flipping based on crystal structures in distinct states, a newly devised in vitro flipping assay, and in vivo studies.
26266984	4	58	from	structures	605:614	arg1	states					628:633	distinct states	619:633	distinct states	619:633	Here we present a mechanism of PglK-catalysed lipid-linked oligosaccharide flipping based on crystal structures in distinct states, a newly devised in vitro flipping assay, and in vivo studies.
26266984	4	58	from	structures	605:614	arg1	assay					670:674	a newly devised in vitro flipping assay	636:674	a newly devised in vitro flipping assay	636:674	Here we present a mechanism of PglK-catalysed lipid-linked oligosaccharide flipping based on crystal structures in distinct states, a newly devised in vitro flipping assay, and in vivo studies.
25750913	5	0	used	used	642:645	arg2	We					639:640	We	639:640	We	639:640	We used glycan microarrays and antibodies to identify the major classes of glycans and glycoproteins present in the cell walls of these sections, and identified the expected decrease in pectin and increase in xylan from the meristematic zone (MS), through the rapid and late elongation zones (REZ, LEZ) to the maturation zone and the rest of the root, including the emerging lateral roots.
25750913	6	1	theme	compositional	1035:1047	arg1	changes					1049:1055	Other compositional changes	1029:1055	Other compositional changes	1029:1055	Other compositional changes included extensin and xyloglucan levels peaking in the REZ and increasing levels of arabinogalactan-proteins (AGP) epitopes from the MS to the LEZ, which remained high through the subsequent mature zones.
25750913	5	2	from	present	740:746	arg1	walls					760:764	the cell walls	751:764	the cell walls of these sections	751:782	We used glycan microarrays and antibodies to identify the major classes of glycans and glycoproteins present in the cell walls of these sections, and identified the expected decrease in pectin and increase in xylan from the meristematic zone (MS), through the rapid and late elongation zones (REZ, LEZ) to the maturation zone and the rest of the root, including the emerging lateral roots.
25750913	11	3	theme	cellulose	1923:1931	arg1	microfibrils					1933:1944	cellulose microfibrils	1923:1944	cellulose microfibrils	1923:1944	The significance of the latter may be related to a role in breaking and re-joining xyloglucan cross-bridges between cellulose microfibrils, a process which is required for wall expansion.
25750913	11	3	theme	cellulose	1923:1931	arg1	process					1949:1955	a process	1947:1955	a process which is required for wall expansion	1947:1992	The significance of the latter may be related to a role in breaking and re-joining xyloglucan cross-bridges between cellulose microfibrils, a process which is required for wall expansion.
25750913	7	4	theme	many	1360:1363	arg1	epitopes					1365:1372	many epitopes	1360:1372	many epitopes	1360:1372	Immuno-staining using the same antibodies identified the tissue and (sub)cellular localization of many epitopes.
25750913	12	5	theme	shorter	2032:2038	arg1	lengths					2045:2051	shorter root lengths	2032:2051	shorter root lengths	2032:2051	Knockdowns of these XTHs resulted in shorter root lengths, confirming a role of the corresponding proteins in root extension growth.
25750913	10	6	theme	oxygen	1672:1677	arg1	ROS					1688:1690	ROS	1688:1690	ROS	1688:1690	These included a large family of peroxidases (which produce the reactive oxygen species (ROS) needed for cell expansion), and three xyloglucan endo-transglycosylase/hydrolase genes (XTH17, XTH18, and XTH19).
25750913	10	6	theme	oxygen	1672:1677	arg1	species					1679:1685	the reactive oxygen species	1659:1685	the reactive oxygen species (ROS) needed for cell expansion	1659:1717	These included a large family of peroxidases (which produce the reactive oxygen species (ROS) needed for cell expansion), and three xyloglucan endo-transglycosylase/hydrolase genes (XTH17, XTH18, and XTH19).
25750913	5	7	theme	meristematic	863:874	arg1	MS					882:883	MS	882:883	MS	882:883	We used glycan microarrays and antibodies to identify the major classes of glycans and glycoproteins present in the cell walls of these sections, and identified the expected decrease in pectin and increase in xylan from the meristematic zone (MS), through the rapid and late elongation zones (REZ, LEZ) to the maturation zone and the rest of the root, including the emerging lateral roots.
25750913	5	7	theme	meristematic	863:874	arg1	zone					876:879	the meristematic zone	859:879	the meristematic zone (MS)	859:884	We used glycan microarrays and antibodies to identify the major classes of glycans and glycoproteins present in the cell walls of these sections, and identified the expected decrease in pectin and increase in xylan from the meristematic zone (MS), through the rapid and late elongation zones (REZ, LEZ) to the maturation zone and the rest of the root, including the emerging lateral roots.
25750913	5	8	theme	major	697:701	arg1	classes					703:709	the major classes	693:709	the major classes of glycans and glycoproteins present in the cell walls of these sections, and identified the expected decrease in pectin and increase in xylan from the meristematic zone (MS), through the rapid and late elongation zones (REZ, LEZ) to the maturation zone and the rest of the root, including the emerging lateral roots	693:1026	We used glycan microarrays and antibodies to identify the major classes of glycans and glycoproteins present in the cell walls of these sections, and identified the expected decrease in pectin and increase in xylan from the meristematic zone (MS), through the rapid and late elongation zones (REZ, LEZ) to the maturation zone and the rest of the root, including the emerging lateral roots.
25750913	10	9	dep	genes	1774:1778	arg1	XTH18					1788:1792	XTH18	1788:1792	XTH18	1788:1792	These included a large family of peroxidases (which produce the reactive oxygen species (ROS) needed for cell expansion), and three xyloglucan endo-transglycosylase/hydrolase genes (XTH17, XTH18, and XTH19).
25750913	10	9	dep	genes	1774:1778	arg1	XTH19					1799:1803	XTH19	1799:1803	XTH19	1799:1803	These included a large family of peroxidases (which produce the reactive oxygen species (ROS) needed for cell expansion), and three xyloglucan endo-transglycosylase/hydrolase genes (XTH17, XTH18, and XTH19).
25750913	10	9	dep	genes	1774:1778	arg1	XTH17					1781:1785	XTH17	1781:1785	XTH17	1781:1785	These included a large family of peroxidases (which produce the reactive oxygen species (ROS) needed for cell expansion), and three xyloglucan endo-transglycosylase/hydrolase genes (XTH17, XTH18, and XTH19).
25750913	10	9	dep	genes	1774:1778	arg1	genes					1774:1778	three xyloglucan endo-transglycosylase/hydrolase genes	1725:1778	three xyloglucan endo-transglycosylase/hydrolase genes (XTH17, XTH18, and XTH19)	1725:1804	These included a large family of peroxidases (which produce the reactive oxygen species (ROS) needed for cell expansion), and three xyloglucan endo-transglycosylase/hydrolase genes (XTH17, XTH18, and XTH19).
25750913	5	10	theme	emerging	1005:1012	arg1	roots					1022:1026	the emerging lateral roots	1001:1026	the emerging lateral roots	1001:1026	We used glycan microarrays and antibodies to identify the major classes of glycans and glycoproteins present in the cell walls of these sections, and identified the expected decrease in pectin and increase in xylan from the meristematic zone (MS), through the rapid and late elongation zones (REZ, LEZ) to the maturation zone and the rest of the root, including the emerging lateral roots.
25750913	11	11	from	role	1858:1861	arg1	breaking					1866:1873	breaking	1866:1873	breaking	1866:1873	The significance of the latter may be related to a role in breaking and re-joining xyloglucan cross-bridges between cellulose microfibrils, a process which is required for wall expansion.
25750913	11	11	from	role	1858:1861	arg1	cross-bridges					1901:1913	re-joining xyloglucan cross-bridges	1879:1913	re-joining xyloglucan cross-bridges between cellulose microfibrils, a process which is required for wall expansion	1879:1992	The significance of the latter may be related to a role in breaking and re-joining xyloglucan cross-bridges between cellulose microfibrils, a process which is required for wall expansion.
25750913	6	12	theme	mature	1248:1253	arg1	zones					1255:1259	the subsequent mature zones	1233:1259	the subsequent mature zones	1233:1259	Other compositional changes included extensin and xyloglucan levels peaking in the REZ and increasing levels of arabinogalactan-proteins (AGP) epitopes from the MS to the LEZ, which remained high through the subsequent mature zones.
25750913	5	13	theme	root	985:988	arg1	rest					973:976	the rest	969:976	the rest of the root	969:988	We used glycan microarrays and antibodies to identify the major classes of glycans and glycoproteins present in the cell walls of these sections, and identified the expected decrease in pectin and increase in xylan from the meristematic zone (MS), through the rapid and late elongation zones (REZ, LEZ) to the maturation zone and the rest of the root, including the emerging lateral roots.
25750913	5	13	theme	root	985:988	arg1	zones					925:929	the rapid and late elongation zones	895:929	the rapid and late elongation zones (REZ, LEZ) to the maturation zone	895:963	We used glycan microarrays and antibodies to identify the major classes of glycans and glycoproteins present in the cell walls of these sections, and identified the expected decrease in pectin and increase in xylan from the meristematic zone (MS), through the rapid and late elongation zones (REZ, LEZ) to the maturation zone and the rest of the root, including the emerging lateral roots.
25750913	5	13	theme	root	985:988	arg1	LEZ					937:939	LEZ	937:939	LEZ	937:939	We used glycan microarrays and antibodies to identify the major classes of glycans and glycoproteins present in the cell walls of these sections, and identified the expected decrease in pectin and increase in xylan from the meristematic zone (MS), through the rapid and late elongation zones (REZ, LEZ) to the maturation zone and the rest of the root, including the emerging lateral roots.
25750913	5	14	from	increase	836:843	arg1	xylan					848:852	xylan	848:852	xylan from the meristematic zone (MS)	848:884	We used glycan microarrays and antibodies to identify the major classes of glycans and glycoproteins present in the cell walls of these sections, and identified the expected decrease in pectin and increase in xylan from the meristematic zone (MS), through the rapid and late elongation zones (REZ, LEZ) to the maturation zone and the rest of the root, including the emerging lateral roots.
25750913	5	14	from	increase	836:843	arg1	pectin					825:830	pectin	825:830	pectin	825:830	We used glycan microarrays and antibodies to identify the major classes of glycans and glycoproteins present in the cell walls of these sections, and identified the expected decrease in pectin and increase in xylan from the meristematic zone (MS), through the rapid and late elongation zones (REZ, LEZ) to the maturation zone and the rest of the root, including the emerging lateral roots.
25750913	5	14	from	increase	836:843	arg1	MS					882:883	MS	882:883	MS	882:883	We used glycan microarrays and antibodies to identify the major classes of glycans and glycoproteins present in the cell walls of these sections, and identified the expected decrease in pectin and increase in xylan from the meristematic zone (MS), through the rapid and late elongation zones (REZ, LEZ) to the maturation zone and the rest of the root, including the emerging lateral roots.
25750913	5	14	from	increase	836:843	arg1	zone					876:879	the meristematic zone	859:879	the meristematic zone (MS)	859:884	We used glycan microarrays and antibodies to identify the major classes of glycans and glycoproteins present in the cell walls of these sections, and identified the expected decrease in pectin and increase in xylan from the meristematic zone (MS), through the rapid and late elongation zones (REZ, LEZ) to the maturation zone and the rest of the root, including the emerging lateral roots.
25750913	5	15	theme	rapid	899:903	arg1	LEZ					937:939	LEZ	937:939	LEZ	937:939	We used glycan microarrays and antibodies to identify the major classes of glycans and glycoproteins present in the cell walls of these sections, and identified the expected decrease in pectin and increase in xylan from the meristematic zone (MS), through the rapid and late elongation zones (REZ, LEZ) to the maturation zone and the rest of the root, including the emerging lateral roots.
25750913	5	15	theme	rapid	899:903	arg1	zones					925:929	the rapid and late elongation zones	895:929	the rapid and late elongation zones (REZ, LEZ) to the maturation zone	895:963	We used glycan microarrays and antibodies to identify the major classes of glycans and glycoproteins present in the cell walls of these sections, and identified the expected decrease in pectin and increase in xylan from the meristematic zone (MS), through the rapid and late elongation zones (REZ, LEZ) to the maturation zone and the rest of the root, including the emerging lateral roots.
25750913	11	16	theme	wall	1979:1982	arg1	expansion					1984:1992	wall expansion	1979:1992	wall expansion	1979:1992	The significance of the latter may be related to a role in breaking and re-joining xyloglucan cross-bridges between cellulose microfibrils, a process which is required for wall expansion.
25750913	4	17	dep	biological	616:625	arg1	replicates					627:636	replicates	627:636	replicates	627:636	Sections of roots from five anatomically and functionally defined zones in Arabidopsis thaliana were prepared and divided into three biological replicates.
25750913	2	18	theme	cell	246:249	arg1	composition					256:266	cell wall composition	246:266	cell wall composition	246:266	However, neither analyses of cell wall composition nor transcriptomes on their own can comprehensively reveal which genes and processes are mediating growth and cell elongation rates.
25750913	5	19	from	zone	876:879	arg1	xylan					848:852	xylan	848:852	xylan from the meristematic zone (MS)	848:884	We used glycan microarrays and antibodies to identify the major classes of glycans and glycoproteins present in the cell walls of these sections, and identified the expected decrease in pectin and increase in xylan from the meristematic zone (MS), through the rapid and late elongation zones (REZ, LEZ) to the maturation zone and the rest of the root, including the emerging lateral roots.
25750913	5	19	from	zone	876:879	arg1	increase					836:843	increase	836:843	increase in xylan from the meristematic zone (MS)	836:884	We used glycan microarrays and antibodies to identify the major classes of glycans and glycoproteins present in the cell walls of these sections, and identified the expected decrease in pectin and increase in xylan from the meristematic zone (MS), through the rapid and late elongation zones (REZ, LEZ) to the maturation zone and the rest of the root, including the emerging lateral roots.
25750913	5	19	from	zone	876:879	arg1	decrease					813:820	the expected decrease	800:820	the expected decrease in pectin	800:830	We used glycan microarrays and antibodies to identify the major classes of glycans and glycoproteins present in the cell walls of these sections, and identified the expected decrease in pectin and increase in xylan from the meristematic zone (MS), through the rapid and late elongation zones (REZ, LEZ) to the maturation zone and the rest of the root, including the emerging lateral roots.
25750913	5	20	theme	late	909:912	arg1	LEZ					937:939	LEZ	937:939	LEZ	937:939	We used glycan microarrays and antibodies to identify the major classes of glycans and glycoproteins present in the cell walls of these sections, and identified the expected decrease in pectin and increase in xylan from the meristematic zone (MS), through the rapid and late elongation zones (REZ, LEZ) to the maturation zone and the rest of the root, including the emerging lateral roots.
25750913	5	20	theme	late	909:912	arg1	zones					925:929	the rapid and late elongation zones	895:929	the rapid and late elongation zones (REZ, LEZ) to the maturation zone	895:963	We used glycan microarrays and antibodies to identify the major classes of glycans and glycoproteins present in the cell walls of these sections, and identified the expected decrease in pectin and increase in xylan from the meristematic zone (MS), through the rapid and late elongation zones (REZ, LEZ) to the maturation zone and the rest of the root, including the emerging lateral roots.
25750913	8	21	theme	cell	1424:1427	arg1	walls					1429:1433	epidermal and cortex cell walls	1403:1433	epidermal and cortex cell walls	1403:1433	Extensins were localized in epidermal and cortex cell walls, while AGP glycans were specific to different tissues from root-hair cells to the stele.
25750913	7	22	theme	same	1288:1291	arg1	antibodies					1293:1302	the same antibodies	1284:1302	the same antibodies	1284:1302	Immuno-staining using the same antibodies identified the tissue and (sub)cellular localization of many epitopes.
25750913	0	23	theme	cell	65:68	arg1	walls					70:74	cell walls	65:74	cell walls	65:74	Multi-omics analysis identifies genes mediating the extension of cell walls in the Arabidopsis thaliana root elongation zone.
25750913	7	24	dep	cellular	1335:1342	arg1	tissue					1319:1324	tissue	1319:1324	tissue	1319:1324	Immuno-staining using the same antibodies identified the tissue and (sub)cellular localization of many epitopes.
25750913	5	25	dep	glycans	714:720	arg1	identified					789:798	identified	789:798	identified	789:798	We used glycan microarrays and antibodies to identify the major classes of glycans and glycoproteins present in the cell walls of these sections, and identified the expected decrease in pectin and increase in xylan from the meristematic zone (MS), through the rapid and late elongation zones (REZ, LEZ) to the maturation zone and the rest of the root, including the emerging lateral roots.
25750913	5	25	dep	glycans	714:720	arg1	present					740:746	present	740:746	present	740:746	We used glycan microarrays and antibodies to identify the major classes of glycans and glycoproteins present in the cell walls of these sections, and identified the expected decrease in pectin and increase in xylan from the meristematic zone (MS), through the rapid and late elongation zones (REZ, LEZ) to the maturation zone and the rest of the root, including the emerging lateral roots.
25750913	4	26	theme	roots	495:499	arg1	Sections					483:490	Sections	483:490	Sections of roots from five anatomically and functionally defined zones in Arabidopsis thaliana	483:577	Sections of roots from five anatomically and functionally defined zones in Arabidopsis thaliana were prepared and divided into three biological replicates.
25750913	8	27	theme	AGP	1442:1444	arg1	glycans					1446:1452	AGP glycans	1442:1452	AGP glycans	1442:1452	Extensins were localized in epidermal and cortex cell walls, while AGP glycans were specific to different tissues from root-hair cells to the stele.
25750913	0	28	theme	Arabidopsis	83:93	arg1	zone					120:123	the Arabidopsis thaliana root elongation zone	79:123	the Arabidopsis thaliana root elongation zone	79:123	Multi-omics analysis identifies genes mediating the extension of cell walls in the Arabidopsis thaliana root elongation zone.
25750913	1	29	theme	cell	132:135	arg1	composition					142:152	Plant cell wall composition	126:152	Plant cell wall composition	126:152	Plant cell wall composition is important for regulating growth rates, especially in roots.
25750913	0	30	from	extension	52:60	arg1	zone					120:123	the Arabidopsis thaliana root elongation zone	79:123	the Arabidopsis thaliana root elongation zone	79:123	Multi-omics analysis identifies genes mediating the extension of cell walls in the Arabidopsis thaliana root elongation zone.
25750913	0	31	theme	root	104:107	arg1	zone					120:123	the Arabidopsis thaliana root elongation zone	79:123	the Arabidopsis thaliana root elongation zone	79:123	Multi-omics analysis identifies genes mediating the extension of cell walls in the Arabidopsis thaliana root elongation zone.
25750913	5	32	from	decrease	813:820	arg1	xylan					848:852	xylan	848:852	xylan from the meristematic zone (MS)	848:884	We used glycan microarrays and antibodies to identify the major classes of glycans and glycoproteins present in the cell walls of these sections, and identified the expected decrease in pectin and increase in xylan from the meristematic zone (MS), through the rapid and late elongation zones (REZ, LEZ) to the maturation zone and the rest of the root, including the emerging lateral roots.
25750913	5	32	from	decrease	813:820	arg1	pectin					825:830	pectin	825:830	pectin	825:830	We used glycan microarrays and antibodies to identify the major classes of glycans and glycoproteins present in the cell walls of these sections, and identified the expected decrease in pectin and increase in xylan from the meristematic zone (MS), through the rapid and late elongation zones (REZ, LEZ) to the maturation zone and the rest of the root, including the emerging lateral roots.
25750913	5	32	from	decrease	813:820	arg1	MS					882:883	MS	882:883	MS	882:883	We used glycan microarrays and antibodies to identify the major classes of glycans and glycoproteins present in the cell walls of these sections, and identified the expected decrease in pectin and increase in xylan from the meristematic zone (MS), through the rapid and late elongation zones (REZ, LEZ) to the maturation zone and the rest of the root, including the emerging lateral roots.
25750913	5	32	from	decrease	813:820	arg1	zone					876:879	the meristematic zone	859:879	the meristematic zone (MS)	859:884	We used glycan microarrays and antibodies to identify the major classes of glycans and glycoproteins present in the cell walls of these sections, and identified the expected decrease in pectin and increase in xylan from the meristematic zone (MS), through the rapid and late elongation zones (REZ, LEZ) to the maturation zone and the rest of the root, including the emerging lateral roots.
25750913	0	33	dep	Arabidopsis	83:93	arg1	thaliana					95:102	thaliana	95:102	thaliana	95:102	Multi-omics analysis identifies genes mediating the extension of cell walls in the Arabidopsis thaliana root elongation zone.
25750913	12	34	theme	root	2105:2108	arg1	growth					2120:2125	root extension growth	2105:2125	root extension growth	2105:2125	Knockdowns of these XTHs resulted in shorter root lengths, confirming a role of the corresponding proteins in root extension growth.
25750913	6	35	from	levels	1131:1136	arg1	MS					1190:1191	the MS	1186:1191	the MS	1186:1191	Other compositional changes included extensin and xyloglucan levels peaking in the REZ and increasing levels of arabinogalactan-proteins (AGP) epitopes from the MS to the LEZ, which remained high through the subsequent mature zones.
25750913	2	36	theme	cell	378:381	arg1	elongation					383:392	cell elongation	378:392	cell elongation	378:392	However, neither analyses of cell wall composition nor transcriptomes on their own can comprehensively reveal which genes and processes are mediating growth and cell elongation rates.
25750913	0	37	theme	Multi-omics	0:10	arg1	analysis					12:19	Multi-omics analysis	0:19	Multi-omics analysis	0:19	Multi-omics analysis identifies genes mediating the extension of cell walls in the Arabidopsis thaliana root elongation zone.
25750913	10	38	theme	large	1616:1620	arg1	family					1622:1627	a large family	1614:1627	a large family of peroxidases (which produce the reactive oxygen species (ROS) needed for cell expansion)	1614:1718	These included a large family of peroxidases (which produce the reactive oxygen species (ROS) needed for cell expansion), and three xyloglucan endo-transglycosylase/hydrolase genes (XTH17, XTH18, and XTH19).
25750913	8	39	theme	root-hair	1494:1502	arg1	cells					1504:1508	root-hair cells	1494:1508	root-hair cells to the stele	1494:1521	Extensins were localized in epidermal and cortex cell walls, while AGP glycans were specific to different tissues from root-hair cells to the stele.
25750913	9	40	theme	transcriptome	1528:1540	arg1	analysis					1542:1549	The transcriptome analysis	1524:1549	The transcriptome analysis	1524:1549	The transcriptome analysis found several gene families peaking in the REZ.
25750913	6	41	dep	included	1057:1064	arg1	increasing					1120:1129	increasing	1120:1129	increasing levels of arabinogalactan-proteins (AGP) epitopes from the MS to the LEZ, which remained high through the subsequent mature zones	1120:1259	Other compositional changes included extensin and xyloglucan levels peaking in the REZ and increasing levels of arabinogalactan-proteins (AGP) epitopes from the MS to the LEZ, which remained high through the subsequent mature zones.
25750913	6	41	dep	included	1057:1064	arg1	peaking					1097:1103	peaking	1097:1103	peaking in the REZ	1097:1114	Other compositional changes included extensin and xyloglucan levels peaking in the REZ and increasing levels of arabinogalactan-proteins (AGP) epitopes from the MS to the LEZ, which remained high through the subsequent mature zones.
25750913	5	42	theme	expected	804:811	arg1	decrease					813:820	the expected decrease	800:820	the expected decrease in pectin	800:830	We used glycan microarrays and antibodies to identify the major classes of glycans and glycoproteins present in the cell walls of these sections, and identified the expected decrease in pectin and increase in xylan from the meristematic zone (MS), through the rapid and late elongation zones (REZ, LEZ) to the maturation zone and the rest of the root, including the emerging lateral roots.
25750913	6	43	theme	xyloglucan	1079:1088	arg1	levels					1090:1095	xyloglucan levels	1079:1095	xyloglucan levels	1079:1095	Other compositional changes included extensin and xyloglucan levels peaking in the REZ and increasing levels of arabinogalactan-proteins (AGP) epitopes from the MS to the LEZ, which remained high through the subsequent mature zones.
25750913	4	44	theme	defined	541:547	arg1	zones					549:553	five anatomically and functionally defined zones	506:553	five anatomically and functionally defined zones in Arabidopsis thaliana	506:577	Sections of roots from five anatomically and functionally defined zones in Arabidopsis thaliana were prepared and divided into three biological replicates.
25750913	2	45	theme	composition	256:266	arg1	analyses					234:241	analyses	234:241	analyses of cell wall composition	234:266	However, neither analyses of cell wall composition nor transcriptomes on their own can comprehensively reveal which genes and processes are mediating growth and cell elongation rates.
25750913	5	46	theme	maturation	949:958	arg1	zone					960:963	the maturation zone	945:963	the maturation zone	945:963	We used glycan microarrays and antibodies to identify the major classes of glycans and glycoproteins present in the cell walls of these sections, and identified the expected decrease in pectin and increase in xylan from the meristematic zone (MS), through the rapid and late elongation zones (REZ, LEZ) to the maturation zone and the rest of the root, including the emerging lateral roots.
25750913	11	47	theme	latter	1831:1836	arg1	significance					1811:1822	The significance	1807:1822	The significance of the latter	1807:1836	The significance of the latter may be related to a role in breaking and re-joining xyloglucan cross-bridges between cellulose microfibrils, a process which is required for wall expansion.
25750913	11	47	theme	latter	1831:1836	arg1	related					1845:1851	related	1845:1851	related	1845:1851	The significance of the latter may be related to a role in breaking and re-joining xyloglucan cross-bridges between cellulose microfibrils, a process which is required for wall expansion.
25750913	10	48	theme	reactive	1663:1670	arg1	ROS					1688:1690	ROS	1688:1690	ROS	1688:1690	These included a large family of peroxidases (which produce the reactive oxygen species (ROS) needed for cell expansion), and three xyloglucan endo-transglycosylase/hydrolase genes (XTH17, XTH18, and XTH19).
25750913	10	48	theme	reactive	1663:1670	arg1	species					1679:1685	the reactive oxygen species	1659:1685	the reactive oxygen species (ROS) needed for cell expansion	1659:1717	These included a large family of peroxidases (which produce the reactive oxygen species (ROS) needed for cell expansion), and three xyloglucan endo-transglycosylase/hydrolase genes (XTH17, XTH18, and XTH19).
25750913	10	49	theme	endo-transglycosylase/hydrolase	1742:1772	arg1	XTH18					1788:1792	XTH18	1788:1792	XTH18	1788:1792	These included a large family of peroxidases (which produce the reactive oxygen species (ROS) needed for cell expansion), and three xyloglucan endo-transglycosylase/hydrolase genes (XTH17, XTH18, and XTH19).
25750913	10	49	theme	endo-transglycosylase/hydrolase	1742:1772	arg1	XTH19					1799:1803	XTH19	1799:1803	XTH19	1799:1803	These included a large family of peroxidases (which produce the reactive oxygen species (ROS) needed for cell expansion), and three xyloglucan endo-transglycosylase/hydrolase genes (XTH17, XTH18, and XTH19).
25750913	10	49	theme	endo-transglycosylase/hydrolase	1742:1772	arg1	XTH17					1781:1785	XTH17	1781:1785	XTH17	1781:1785	These included a large family of peroxidases (which produce the reactive oxygen species (ROS) needed for cell expansion), and three xyloglucan endo-transglycosylase/hydrolase genes (XTH17, XTH18, and XTH19).
25750913	10	49	theme	endo-transglycosylase/hydrolase	1742:1772	arg1	genes					1774:1778	three xyloglucan endo-transglycosylase/hydrolase genes	1725:1778	three xyloglucan endo-transglycosylase/hydrolase genes (XTH17, XTH18, and XTH19)	1725:1804	These included a large family of peroxidases (which produce the reactive oxygen species (ROS) needed for cell expansion), and three xyloglucan endo-transglycosylase/hydrolase genes (XTH17, XTH18, and XTH19).
25750913	8	50	from	cells	1504:1508	arg1	tissues					1481:1487	different tissues	1471:1487	different tissues from root-hair cells to the stele	1471:1521	Extensins were localized in epidermal and cortex cell walls, while AGP glycans were specific to different tissues from root-hair cells to the stele.
25750913	6	51	theme	Other	1029:1033	arg1	changes					1049:1055	Other compositional changes	1029:1055	Other compositional changes	1029:1055	Other compositional changes included extensin and xyloglucan levels peaking in the REZ and increasing levels of arabinogalactan-proteins (AGP) epitopes from the MS to the LEZ, which remained high through the subsequent mature zones.
25750913	12	52	theme	proteins	2093:2100	arg1	role					2067:2070	a role	2065:2070	a role of the corresponding proteins in root extension growth	2065:2125	Knockdowns of these XTHs resulted in shorter root lengths, confirming a role of the corresponding proteins in root extension growth.
25750913	11	53	theme	xyloglucan	1890:1899	arg1	cross-bridges					1901:1913	re-joining xyloglucan cross-bridges	1879:1913	re-joining xyloglucan cross-bridges between cellulose microfibrils, a process which is required for wall expansion	1879:1992	The significance of the latter may be related to a role in breaking and re-joining xyloglucan cross-bridges between cellulose microfibrils, a process which is required for wall expansion.
25750913	7	54	theme	epitopes	1365:1372	arg1	localization					1344:1355	the tissue and (sub)cellular localization	1315:1355	the tissue and (sub)cellular localization of many epitopes	1315:1372	Immuno-staining using the same antibodies identified the tissue and (sub)cellular localization of many epitopes.
25750913	5	55	theme	glycan	647:652	arg1	microarrays					654:664	glycan microarrays	647:664	glycan microarrays	647:664	We used glycan microarrays and antibodies to identify the major classes of glycans and glycoproteins present in the cell walls of these sections, and identified the expected decrease in pectin and increase in xylan from the meristematic zone (MS), through the rapid and late elongation zones (REZ, LEZ) to the maturation zone and the rest of the root, including the emerging lateral roots.
25750913	10	56	theme	cell	1704:1707	arg1	expansion					1709:1717	cell expansion	1704:1717	cell expansion	1704:1717	These included a large family of peroxidases (which produce the reactive oxygen species (ROS) needed for cell expansion), and three xyloglucan endo-transglycosylase/hydrolase genes (XTH17, XTH18, and XTH19).
25750913	1	57	theme	growth	182:187	arg1	rates					189:193	growth rates	182:193	growth rates	182:193	Plant cell wall composition is important for regulating growth rates, especially in roots.
25750913	12	58	theme	root	2040:2043	arg1	lengths					2045:2051	shorter root lengths	2032:2051	shorter root lengths	2032:2051	Knockdowns of these XTHs resulted in shorter root lengths, confirming a role of the corresponding proteins in root extension growth.
25750913	5	59	theme	glycans	714:720	arg1	classes					703:709	the major classes	693:709	the major classes of glycans and glycoproteins present in the cell walls of these sections, and identified the expected decrease in pectin and increase in xylan from the meristematic zone (MS), through the rapid and late elongation zones (REZ, LEZ) to the maturation zone and the rest of the root, including the emerging lateral roots	693:1026	We used glycan microarrays and antibodies to identify the major classes of glycans and glycoproteins present in the cell walls of these sections, and identified the expected decrease in pectin and increase in xylan from the meristematic zone (MS), through the rapid and late elongation zones (REZ, LEZ) to the maturation zone and the rest of the root, including the emerging lateral roots.
25750913	7	60	theme	cellular	1335:1342	arg1	localization					1344:1355	the tissue and (sub)cellular localization	1315:1355	the tissue and (sub)cellular localization of many epitopes	1315:1372	Immuno-staining using the same antibodies identified the tissue and (sub)cellular localization of many epitopes.
25750913	5	61	theme	glycoproteins	726:738	arg1	classes					703:709	the major classes	693:709	the major classes of glycans and glycoproteins present in the cell walls of these sections, and identified the expected decrease in pectin and increase in xylan from the meristematic zone (MS), through the rapid and late elongation zones (REZ, LEZ) to the maturation zone and the rest of the root, including the emerging lateral roots	693:1026	We used glycan microarrays and antibodies to identify the major classes of glycans and glycoproteins present in the cell walls of these sections, and identified the expected decrease in pectin and increase in xylan from the meristematic zone (MS), through the rapid and late elongation zones (REZ, LEZ) to the maturation zone and the rest of the root, including the emerging lateral roots.
25750913	5	62	from	walls	760:764	arg1	present					740:746	present	740:746	present	740:746	We used glycan microarrays and antibodies to identify the major classes of glycans and glycoproteins present in the cell walls of these sections, and identified the expected decrease in pectin and increase in xylan from the meristematic zone (MS), through the rapid and late elongation zones (REZ, LEZ) to the maturation zone and the rest of the root, including the emerging lateral roots.
25750913	8	63	theme	epidermal	1403:1411	arg1	walls					1429:1433	epidermal and cortex cell walls	1403:1433	epidermal and cortex cell walls	1403:1433	Extensins were localized in epidermal and cortex cell walls, while AGP glycans were specific to different tissues from root-hair cells to the stele.
25750913	7	64	theme	sub	1331:1333	arg1	localization					1344:1355	the tissue and (sub)cellular localization	1315:1355	the tissue and (sub)cellular localization of many epitopes	1315:1372	Immuno-staining using the same antibodies identified the tissue and (sub)cellular localization of many epitopes.
25750913	12	65	theme	XTHs	2015:2018	arg1	Knockdowns					1995:2004	Knockdowns	1995:2004	Knockdowns of these XTHs	1995:2018	Knockdowns of these XTHs resulted in shorter root lengths, confirming a role of the corresponding proteins in root extension growth.
25750913	1	66	from	roots	210:214	arg1	important					157:165	important	157:165	important	157:165	Plant cell wall composition is important for regulating growth rates, especially in roots.
25750913	8	67	theme	cortex	1417:1422	arg1	walls					1429:1433	epidermal and cortex cell walls	1403:1433	epidermal and cortex cell walls	1403:1433	Extensins were localized in epidermal and cortex cell walls, while AGP glycans were specific to different tissues from root-hair cells to the stele.
25750913	6	68	theme	epitopes	1172:1179	arg1	levels					1131:1136	levels	1131:1136	levels of arabinogalactan-proteins (AGP) epitopes from the MS	1131:1191	Other compositional changes included extensin and xyloglucan levels peaking in the REZ and increasing levels of arabinogalactan-proteins (AGP) epitopes from the MS to the LEZ, which remained high through the subsequent mature zones.
25750913	6	69	theme	subsequent	1237:1246	arg1	zones					1255:1259	the subsequent mature zones	1233:1259	the subsequent mature zones	1233:1259	Other compositional changes included extensin and xyloglucan levels peaking in the REZ and increasing levels of arabinogalactan-proteins (AGP) epitopes from the MS to the LEZ, which remained high through the subsequent mature zones.
25750913	2	70	theme	wall	251:254	arg1	composition					256:266	cell wall composition	246:266	cell wall composition	246:266	However, neither analyses of cell wall composition nor transcriptomes on their own can comprehensively reveal which genes and processes are mediating growth and cell elongation rates.
25750913	1	71	theme	Plant	126:130	arg1	composition					142:152	Plant cell wall composition	126:152	Plant cell wall composition	126:152	Plant cell wall composition is important for regulating growth rates, especially in roots.
25750913	8	72	located	localized	1390:1398	arg2	Extensins					1375:1383	Extensins	1375:1383	Extensins	1375:1383	Extensins were localized in epidermal and cortex cell walls, while AGP glycans were specific to different tissues from root-hair cells to the stele.
25750913	8	72	located	localized	1390:1398	arg1	walls					1429:1433	epidermal and cortex cell walls	1403:1433	epidermal and cortex cell walls	1403:1433	Extensins were localized in epidermal and cortex cell walls, while AGP glycans were specific to different tissues from root-hair cells to the stele.
25750913	3	73	contain	carrying	436:443	arg2	analyses					458:465	multiple analyses	449:465	multiple analyses	449:465	This study reveals the benefits of carrying out multiple analyses in combination.
25750913	3	73	contain	carrying	436:443	arg1	benefits					424:431	the benefits	420:431	the benefits of carrying out multiple analyses in combination	420:480	This study reveals the benefits of carrying out multiple analyses in combination.
25750913	5	74	gly	glycoproteins	726:738	arg1	glycoproteins					726:738	glycoproteins	726:738	glycoproteins	726:738	We used glycan microarrays and antibodies to identify the major classes of glycans and glycoproteins present in the cell walls of these sections, and identified the expected decrease in pectin and increase in xylan from the meristematic zone (MS), through the rapid and late elongation zones (REZ, LEZ) to the maturation zone and the rest of the root, including the emerging lateral roots.
25750913	6	75	from	MS	1190:1191	arg1	levels					1131:1136	levels	1131:1136	levels of arabinogalactan-proteins (AGP) epitopes from the MS	1131:1191	Other compositional changes included extensin and xyloglucan levels peaking in the REZ and increasing levels of arabinogalactan-proteins (AGP) epitopes from the MS to the LEZ, which remained high through the subsequent mature zones.
25750913	6	75	from	MS	1190:1191	arg1	epitopes					1172:1179	arabinogalactan-proteins (AGP) epitopes	1141:1179	arabinogalactan-proteins (AGP) epitopes from the MS	1141:1191	Other compositional changes included extensin and xyloglucan levels peaking in the REZ and increasing levels of arabinogalactan-proteins (AGP) epitopes from the MS to the LEZ, which remained high through the subsequent mature zones.
25750913	0	76	theme	walls	70:74	arg1	extension					52:60	the extension	48:60	the extension of cell walls in the Arabidopsis thaliana root elongation zone	48:123	Multi-omics analysis identifies genes mediating the extension of cell walls in the Arabidopsis thaliana root elongation zone.
25750913	10	77	theme	xyloglucan	1731:1740	arg1	XTH18					1788:1792	XTH18	1788:1792	XTH18	1788:1792	These included a large family of peroxidases (which produce the reactive oxygen species (ROS) needed for cell expansion), and three xyloglucan endo-transglycosylase/hydrolase genes (XTH17, XTH18, and XTH19).
25750913	10	77	theme	xyloglucan	1731:1740	arg1	XTH19					1799:1803	XTH19	1799:1803	XTH19	1799:1803	These included a large family of peroxidases (which produce the reactive oxygen species (ROS) needed for cell expansion), and three xyloglucan endo-transglycosylase/hydrolase genes (XTH17, XTH18, and XTH19).
25750913	10	77	theme	xyloglucan	1731:1740	arg1	XTH17					1781:1785	XTH17	1781:1785	XTH17	1781:1785	These included a large family of peroxidases (which produce the reactive oxygen species (ROS) needed for cell expansion), and three xyloglucan endo-transglycosylase/hydrolase genes (XTH17, XTH18, and XTH19).
25750913	10	77	theme	xyloglucan	1731:1740	arg1	genes					1774:1778	three xyloglucan endo-transglycosylase/hydrolase genes	1725:1778	three xyloglucan endo-transglycosylase/hydrolase genes (XTH17, XTH18, and XTH19)	1725:1804	These included a large family of peroxidases (which produce the reactive oxygen species (ROS) needed for cell expansion), and three xyloglucan endo-transglycosylase/hydrolase genes (XTH17, XTH18, and XTH19).
25750913	1	78	theme	wall	137:140	arg1	composition					142:152	Plant cell wall composition	126:152	Plant cell wall composition	126:152	Plant cell wall composition is important for regulating growth rates, especially in roots.
25750913	2	79	from	transcriptomes	272:285	arg1	own					296:298	own	296:298	own	296:298	However, neither analyses of cell wall composition nor transcriptomes on their own can comprehensively reveal which genes and processes are mediating growth and cell elongation rates.
25750913	6	80	theme	arabinogalactan-proteins	1141:1164	arg1	epitopes					1172:1179	arabinogalactan-proteins (AGP) epitopes	1141:1179	arabinogalactan-proteins (AGP) epitopes from the MS	1141:1191	Other compositional changes included extensin and xyloglucan levels peaking in the REZ and increasing levels of arabinogalactan-proteins (AGP) epitopes from the MS to the LEZ, which remained high through the subsequent mature zones.
25750913	5	81	theme	sections	775:782	arg1	walls					760:764	the cell walls	751:764	the cell walls of these sections	751:782	We used glycan microarrays and antibodies to identify the major classes of glycans and glycoproteins present in the cell walls of these sections, and identified the expected decrease in pectin and increase in xylan from the meristematic zone (MS), through the rapid and late elongation zones (REZ, LEZ) to the maturation zone and the rest of the root, including the emerging lateral roots.
25750913	9	82	theme	gene	1565:1568	arg1	families					1570:1577	several gene families	1557:1577	several gene families peaking in the REZ	1557:1596	The transcriptome analysis found several gene families peaking in the REZ.
25750913	1	83	from	important	157:165	arg1	roots					210:214	roots	210:214	roots	210:214	Plant cell wall composition is important for regulating growth rates, especially in roots.
25750913	0	84	theme	elongation	109:118	arg1	zone					120:123	the Arabidopsis thaliana root elongation zone	79:123	the Arabidopsis thaliana root elongation zone	79:123	Multi-omics analysis identifies genes mediating the extension of cell walls in the Arabidopsis thaliana root elongation zone.
25750913	8	85	theme	different	1471:1479	arg1	tissues					1481:1487	different tissues	1471:1487	different tissues from root-hair cells to the stele	1471:1521	Extensins were localized in epidermal and cortex cell walls, while AGP glycans were specific to different tissues from root-hair cells to the stele.
25750913	2	86	theme	elongation	383:392	arg1	rates					394:398	growth and cell elongation rates	367:398	growth and cell elongation rates	367:398	However, neither analyses of cell wall composition nor transcriptomes on their own can comprehensively reveal which genes and processes are mediating growth and cell elongation rates.
25750913	5	87	theme	elongation	914:923	arg1	LEZ					937:939	LEZ	937:939	LEZ	937:939	We used glycan microarrays and antibodies to identify the major classes of glycans and glycoproteins present in the cell walls of these sections, and identified the expected decrease in pectin and increase in xylan from the meristematic zone (MS), through the rapid and late elongation zones (REZ, LEZ) to the maturation zone and the rest of the root, including the emerging lateral roots.
25750913	5	87	theme	elongation	914:923	arg1	zones					925:929	the rapid and late elongation zones	895:929	the rapid and late elongation zones (REZ, LEZ) to the maturation zone	895:963	We used glycan microarrays and antibodies to identify the major classes of glycans and glycoproteins present in the cell walls of these sections, and identified the expected decrease in pectin and increase in xylan from the meristematic zone (MS), through the rapid and late elongation zones (REZ, LEZ) to the maturation zone and the rest of the root, including the emerging lateral roots.
25750913	4	88	theme	Arabidopsis	558:568	arg1	thaliana					570:577	Arabidopsis thaliana	558:577	Arabidopsis thaliana	558:577	Sections of roots from five anatomically and functionally defined zones in Arabidopsis thaliana were prepared and divided into three biological replicates.
25750913	5	89	theme	lateral	1014:1020	arg1	roots					1022:1026	the emerging lateral roots	1001:1026	the emerging lateral roots	1001:1026	We used glycan microarrays and antibodies to identify the major classes of glycans and glycoproteins present in the cell walls of these sections, and identified the expected decrease in pectin and increase in xylan from the meristematic zone (MS), through the rapid and late elongation zones (REZ, LEZ) to the maturation zone and the rest of the root, including the emerging lateral roots.
25750913	12	90	theme	extension	2110:2118	arg1	growth					2120:2125	root extension growth	2105:2125	root extension growth	2105:2125	Knockdowns of these XTHs resulted in shorter root lengths, confirming a role of the corresponding proteins in root extension growth.
25750913	2	91	theme	growth	367:372	arg1	rates					394:398	growth and cell elongation rates	367:398	growth and cell elongation rates	367:398	However, neither analyses of cell wall composition nor transcriptomes on their own can comprehensively reveal which genes and processes are mediating growth and cell elongation rates.
25750913	4	92	from	zones	549:553	arg1	Sections					483:490	Sections	483:490	Sections of roots from five anatomically and functionally defined zones in Arabidopsis thaliana	483:577	Sections of roots from five anatomically and functionally defined zones in Arabidopsis thaliana were prepared and divided into three biological replicates.
25750913	4	92	from	zones	549:553	arg1	thaliana					570:577	Arabidopsis thaliana	558:577	Arabidopsis thaliana	558:577	Sections of roots from five anatomically and functionally defined zones in Arabidopsis thaliana were prepared and divided into three biological replicates.
25750913	3	93	theme	multiple	449:456	arg1	analyses					458:465	multiple analyses	449:465	multiple analyses	449:465	This study reveals the benefits of carrying out multiple analyses in combination.
25750913	12	94	theme	corresponding	2079:2091	arg1	proteins					2093:2100	the corresponding proteins	2075:2100	the corresponding proteins	2075:2100	Knockdowns of these XTHs resulted in shorter root lengths, confirming a role of the corresponding proteins in root extension growth.
25750913	10	95	theme	peroxidases	1632:1642	arg1	family					1622:1627	a large family	1614:1627	a large family of peroxidases (which produce the reactive oxygen species (ROS) needed for cell expansion)	1614:1718	These included a large family of peroxidases (which produce the reactive oxygen species (ROS) needed for cell expansion), and three xyloglucan endo-transglycosylase/hydrolase genes (XTH17, XTH18, and XTH19).
25750913	10	95	theme	peroxidases	1632:1642	arg1	XTH18					1788:1792	XTH18	1788:1792	XTH18	1788:1792	These included a large family of peroxidases (which produce the reactive oxygen species (ROS) needed for cell expansion), and three xyloglucan endo-transglycosylase/hydrolase genes (XTH17, XTH18, and XTH19).
25750913	10	95	theme	peroxidases	1632:1642	arg1	XTH19					1799:1803	XTH19	1799:1803	XTH19	1799:1803	These included a large family of peroxidases (which produce the reactive oxygen species (ROS) needed for cell expansion), and three xyloglucan endo-transglycosylase/hydrolase genes (XTH17, XTH18, and XTH19).
25750913	10	95	theme	peroxidases	1632:1642	arg1	XTH17					1781:1785	XTH17	1781:1785	XTH17	1781:1785	These included a large family of peroxidases (which produce the reactive oxygen species (ROS) needed for cell expansion), and three xyloglucan endo-transglycosylase/hydrolase genes (XTH17, XTH18, and XTH19).
25750913	10	95	theme	peroxidases	1632:1642	arg1	genes					1774:1778	three xyloglucan endo-transglycosylase/hydrolase genes	1725:1778	three xyloglucan endo-transglycosylase/hydrolase genes (XTH17, XTH18, and XTH19)	1725:1804	These included a large family of peroxidases (which produce the reactive oxygen species (ROS) needed for cell expansion), and three xyloglucan endo-transglycosylase/hydrolase genes (XTH17, XTH18, and XTH19).
25750913	9	96	theme	several	1557:1563	arg1	families					1570:1577	several gene families	1557:1577	several gene families peaking in the REZ	1557:1596	The transcriptome analysis found several gene families peaking in the REZ.
25750913	5	97	theme	cell	755:758	arg1	walls					760:764	the cell walls	751:764	the cell walls of these sections	751:782	We used glycan microarrays and antibodies to identify the major classes of glycans and glycoproteins present in the cell walls of these sections, and identified the expected decrease in pectin and increase in xylan from the meristematic zone (MS), through the rapid and late elongation zones (REZ, LEZ) to the maturation zone and the rest of the root, including the emerging lateral roots.
25750913	11	98	theme	re-joining	1879:1888	arg1	cross-bridges					1901:1913	re-joining xyloglucan cross-bridges	1879:1913	re-joining xyloglucan cross-bridges between cellulose microfibrils, a process which is required for wall expansion	1879:1992	The significance of the latter may be related to a role in breaking and re-joining xyloglucan cross-bridges between cellulose microfibrils, a process which is required for wall expansion.
25750913	12	99	from	role	2067:2070	arg1	growth					2120:2125	root extension growth	2105:2125	root extension growth	2105:2125	Knockdowns of these XTHs resulted in shorter root lengths, confirming a role of the corresponding proteins in root extension growth.
29050583	3	0	theme	serum	1030:1034	arg1	albumin					1036:1042	bovine serum albumin	1023:1042	bovine serum albumin	1023:1042	Compared to a widely used form of CheSePs stabilized and coated by bovine serum albumin, EPS-BioSePs exhibited better antioxidant properties on scavenging DPPH, superoxide and ABTS free radicals, but not hydroxyl radical.
29050583	2	1	theme	electron	678:685	arg1	TEM					699:701	TEM	699:701	TEM	699:701	Characterization by energy dispersive X-ray spectrometry (EDX), scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FT-IR), size measurement and chemical composition analysis verified that EPS-BioSePs exhibited a monodispersed and homogeneous spherical structure 293.73±4.03nm in size.
29050583	2	1	theme	electron	678:685	arg1	microscopy					687:696	transmission electron microscopy	665:696	transmission electron microscopy (TEM)	665:702	Characterization by energy dispersive X-ray spectrometry (EDX), scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FT-IR), size measurement and chemical composition analysis verified that EPS-BioSePs exhibited a monodispersed and homogeneous spherical structure 293.73±4.03nm in size.
29050583	3	2	dep	CheSePs	990:996	arg1	coated					1013:1018	coated	1013:1018	coated by bovine serum albumin	1013:1042	Compared to a widely used form of CheSePs stabilized and coated by bovine serum albumin, EPS-BioSePs exhibited better antioxidant properties on scavenging DPPH, superoxide and ABTS free radicals, but not hydroxyl radical.
29050583	3	2	dep	CheSePs	990:996	arg1	stabilized					998:1007	stabilized	998:1007	stabilized	998:1007	Compared to a widely used form of CheSePs stabilized and coated by bovine serum albumin, EPS-BioSePs exhibited better antioxidant properties on scavenging DPPH, superoxide and ABTS free radicals, but not hydroxyl radical.
29050583	2	3	theme	spherical	913:921	arg1	293.73±4.03nm					933:945	a monodispersed and homogeneous spherical structure 293.73±4.03nm	881:945	a monodispersed and homogeneous spherical structure 293.73±4.03nm	881:945	Characterization by energy dispersive X-ray spectrometry (EDX), scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FT-IR), size measurement and chemical composition analysis verified that EPS-BioSePs exhibited a monodispersed and homogeneous spherical structure 293.73±4.03nm in size.
29050583	0	4	theme	particles	105:113	arg1	cytoprotection					43:56	cytoprotection	43:56	cytoprotection	43:56	Characterization, antioxidant property and cytoprotection of exopolysaccharide-capped elemental selenium particles synthesized by Bacillus paralicheniformis SR14.
29050583	0	4	theme	particles	105:113	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization, antioxidant property and cytoprotection of exopolysaccharide-capped elemental selenium particles synthesized by Bacillus paralicheniformis SR14.
29050583	0	4	theme	particles	105:113	arg1	property					30:37	antioxidant property	18:37	antioxidant property	18:37	Characterization, antioxidant property and cytoprotection of exopolysaccharide-capped elemental selenium particles synthesized by Bacillus paralicheniformis SR14.
29050583	2	5	theme	transmission	665:676	arg1	TEM					699:701	TEM	699:701	TEM	699:701	Characterization by energy dispersive X-ray spectrometry (EDX), scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FT-IR), size measurement and chemical composition analysis verified that EPS-BioSePs exhibited a monodispersed and homogeneous spherical structure 293.73±4.03nm in size.
29050583	2	5	theme	transmission	665:676	arg1	microscopy					687:696	transmission electron microscopy	665:696	transmission electron microscopy (TEM)	665:702	Characterization by energy dispersive X-ray spectrometry (EDX), scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FT-IR), size measurement and chemical composition analysis verified that EPS-BioSePs exhibited a monodispersed and homogeneous spherical structure 293.73±4.03nm in size.
29050583	3	6	theme	scavenging	1100:1109	arg1	radicals					1142:1149	scavenging DPPH, superoxide and ABTS free radicals	1100:1149	radicals	1142:1149	Compared to a widely used form of CheSePs stabilized and coated by bovine serum albumin, EPS-BioSePs exhibited better antioxidant properties on scavenging DPPH, superoxide and ABTS free radicals, but not hydroxyl radical.
29050583	2	7	theme	homogeneous	901:911	arg1	293.73±4.03nm					933:945	a monodispersed and homogeneous spherical structure 293.73±4.03nm	881:945	a monodispersed and homogeneous spherical structure 293.73±4.03nm	881:945	Characterization by energy dispersive X-ray spectrometry (EDX), scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FT-IR), size measurement and chemical composition analysis verified that EPS-BioSePs exhibited a monodispersed and homogeneous spherical structure 293.73±4.03nm in size.
29050583	1	8	theme	purifying	297:305	arg1	polysaccharides					307:321	purifying polysaccharides	297:321	purifying polysaccharides	297:321	Instead of using existing methods to chemically synthesize elemental selenium particles (CheSePs), which first require separating and purifying polysaccharides or proteins and adding extra reducing agent, this study applied a novel method to directly assemble exopolysaccharide-capped biogenic elemental selenium particles (EPS-BioSePs) by Bacillus paralicheniformis SR14 during the metabolic process.
29050583	0	9	theme	Bacillus	130:137	arg1	SR14					157:160	Bacillus paralicheniformis SR14	130:160	Bacillus paralicheniformis SR14	130:160	Characterization, antioxidant property and cytoprotection of exopolysaccharide-capped elemental selenium particles synthesized by Bacillus paralicheniformis SR14.
29050583	1	10	theme	novel	389:393	arg1	method					395:400	a novel method	387:400	a novel method to directly assemble exopolysaccharide-capped biogenic elemental selenium particles (EPS-BioSePs) by Bacillus paralicheniformis SR14 during the metabolic process	387:562	Instead of using existing methods to chemically synthesize elemental selenium particles (CheSePs), which first require separating and purifying polysaccharides or proteins and adding extra reducing agent, this study applied a novel method to directly assemble exopolysaccharide-capped biogenic elemental selenium particles (EPS-BioSePs) by Bacillus paralicheniformis SR14 during the metabolic process.
29050583	3	11	theme	free	1137:1140	arg1	radicals					1142:1149	scavenging DPPH, superoxide and ABTS free radicals	1100:1149	radicals	1142:1149	Compared to a widely used form of CheSePs stabilized and coated by bovine serum albumin, EPS-BioSePs exhibited better antioxidant properties on scavenging DPPH, superoxide and ABTS free radicals, but not hydroxyl radical.
29050583	1	12	theme	Bacillus	503:510	arg1	SR14					530:533	Bacillus paralicheniformis SR14	503:533	Bacillus paralicheniformis SR14	503:533	Instead of using existing methods to chemically synthesize elemental selenium particles (CheSePs), which first require separating and purifying polysaccharides or proteins and adding extra reducing agent, this study applied a novel method to directly assemble exopolysaccharide-capped biogenic elemental selenium particles (EPS-BioSePs) by Bacillus paralicheniformis SR14 during the metabolic process.
29050583	5	13	with	particles	1673:1681	arg1	applications					1698:1709	potential applications	1688:1709	potential applications as antioxidants	1688:1725	These results suggested that this new form of selenium possessed great antioxidant property and cytoprotection and exopolysaccharide-producing bacteria could gradually become an appropriate choice to synthesize biogenic elemental selenium particles with potential applications as antioxidants.
29050583	4	14	theme	ROS	1418:1420	arg1	generation					1422:1431	ROS generation	1418:1431	ROS generation	1418:1431	In vitro experiments with porcine jejunum epithelial (IPEC-J2) cells also indicated a significant cytoprotection of EPS-BioSePs against hydrogen peroxide-induced oxidative stress, as exhibited by cell viability reduction and suppression of ROS generation.
29050583	5	15	theme	elemental	1654:1662	arg1	selenium					1664:1671	biogenic elemental selenium	1645:1671	biogenic elemental selenium particles with potential applications as antioxidants	1645:1725	These results suggested that this new form of selenium possessed great antioxidant property and cytoprotection and exopolysaccharide-producing bacteria could gradually become an appropriate choice to synthesize biogenic elemental selenium particles with potential applications as antioxidants.
29050583	1	16	theme	paralicheniformis	512:528	arg1	SR14					530:533	Bacillus paralicheniformis SR14	503:533	Bacillus paralicheniformis SR14	503:533	Instead of using existing methods to chemically synthesize elemental selenium particles (CheSePs), which first require separating and purifying polysaccharides or proteins and adding extra reducing agent, this study applied a novel method to directly assemble exopolysaccharide-capped biogenic elemental selenium particles (EPS-BioSePs) by Bacillus paralicheniformis SR14 during the metabolic process.
29050583	2	17	theme	chemical	815:822	arg1	analysis					836:843	chemical composition analysis	815:843	chemical composition analysis	815:843	Characterization by energy dispersive X-ray spectrometry (EDX), scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FT-IR), size measurement and chemical composition analysis verified that EPS-BioSePs exhibited a monodispersed and homogeneous spherical structure 293.73±4.03nm in size.
29050583	4	18	theme	epithelial	1220:1229	arg1	cells					1241:1245	porcine jejunum epithelial (IPEC-J2) cells	1204:1245	porcine jejunum epithelial (IPEC-J2) cells	1204:1245	In vitro experiments with porcine jejunum epithelial (IPEC-J2) cells also indicated a significant cytoprotection of EPS-BioSePs against hydrogen peroxide-induced oxidative stress, as exhibited by cell viability reduction and suppression of ROS generation.
29050583	2	19	theme	X-ray	603:607	arg1	EDX					623:625	EDX	623:625	EDX	623:625	Characterization by energy dispersive X-ray spectrometry (EDX), scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FT-IR), size measurement and chemical composition analysis verified that EPS-BioSePs exhibited a monodispersed and homogeneous spherical structure 293.73±4.03nm in size.
29050583	2	19	theme	X-ray	603:607	arg1	spectrometry					609:620	energy dispersive X-ray spectrometry	585:620	energy dispersive X-ray spectrometry (EDX)	585:626	Characterization by energy dispersive X-ray spectrometry (EDX), scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FT-IR), size measurement and chemical composition analysis verified that EPS-BioSePs exhibited a monodispersed and homogeneous spherical structure 293.73±4.03nm in size.
29050583	4	20	theme	jejunum	1212:1218	arg1	cells					1241:1245	porcine jejunum epithelial (IPEC-J2) cells	1204:1245	porcine jejunum epithelial (IPEC-J2) cells	1204:1245	In vitro experiments with porcine jejunum epithelial (IPEC-J2) cells also indicated a significant cytoprotection of EPS-BioSePs against hydrogen peroxide-induced oxidative stress, as exhibited by cell viability reduction and suppression of ROS generation.
29050583	3	21	theme	CheSePs	990:996	arg1	form					982:985	a widely used form	968:985	a widely used form of CheSePs stabilized and coated by bovine serum albumin	968:1042	Compared to a widely used form of CheSePs stabilized and coated by bovine serum albumin, EPS-BioSePs exhibited better antioxidant properties on scavenging DPPH, superoxide and ABTS free radicals, but not hydroxyl radical.
29050583	2	22	theme	X-ray	705:709	arg1	XPS					739:741	XPS	739:741	XPS	739:741	Characterization by energy dispersive X-ray spectrometry (EDX), scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FT-IR), size measurement and chemical composition analysis verified that EPS-BioSePs exhibited a monodispersed and homogeneous spherical structure 293.73±4.03nm in size.
29050583	2	22	theme	X-ray	705:709	arg1	spectroscopy					725:736	X-ray photoelectron spectroscopy	705:736	X-ray photoelectron spectroscopy (XPS)	705:742	Characterization by energy dispersive X-ray spectrometry (EDX), scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FT-IR), size measurement and chemical composition analysis verified that EPS-BioSePs exhibited a monodispersed and homogeneous spherical structure 293.73±4.03nm in size.
29050583	0	23	theme	paralicheniformis	139:155	arg1	SR14					157:160	Bacillus paralicheniformis SR14	130:160	Bacillus paralicheniformis SR14	130:160	Characterization, antioxidant property and cytoprotection of exopolysaccharide-capped elemental selenium particles synthesized by Bacillus paralicheniformis SR14.
29050583	4	24	theme	EPS-BioSePs	1294:1304	arg1	cytoprotection					1276:1289	a significant cytoprotection	1262:1289	a significant cytoprotection of EPS-BioSePs against hydrogen peroxide-induced oxidative stress	1262:1355	In vitro experiments with porcine jejunum epithelial (IPEC-J2) cells also indicated a significant cytoprotection of EPS-BioSePs against hydrogen peroxide-induced oxidative stress, as exhibited by cell viability reduction and suppression of ROS generation.
29050583	3	25	theme	used	977:980	arg1	form					982:985	a widely used form	968:985	a widely used form of CheSePs stabilized and coated by bovine serum albumin	968:1042	Compared to a widely used form of CheSePs stabilized and coated by bovine serum albumin, EPS-BioSePs exhibited better antioxidant properties on scavenging DPPH, superoxide and ABTS free radicals, but not hydroxyl radical.
29050583	1	26	theme	elemental	222:230	arg1	CheSePs					252:258	CheSePs	252:258	CheSePs	252:258	Instead of using existing methods to chemically synthesize elemental selenium particles (CheSePs), which first require separating and purifying polysaccharides or proteins and adding extra reducing agent, this study applied a novel method to directly assemble exopolysaccharide-capped biogenic elemental selenium particles (EPS-BioSePs) by Bacillus paralicheniformis SR14 during the metabolic process.
29050583	1	26	theme	elemental	222:230	arg1	particles					241:249	elemental selenium particles	222:249	elemental selenium particles (CheSePs)	222:259	Instead of using existing methods to chemically synthesize elemental selenium particles (CheSePs), which first require separating and purifying polysaccharides or proteins and adding extra reducing agent, this study applied a novel method to directly assemble exopolysaccharide-capped biogenic elemental selenium particles (EPS-BioSePs) by Bacillus paralicheniformis SR14 during the metabolic process.
29050583	4	27	theme	generation	1422:1431	arg1	reduction					1389:1397	cell viability reduction	1374:1397	cell viability reduction	1374:1397	In vitro experiments with porcine jejunum epithelial (IPEC-J2) cells also indicated a significant cytoprotection of EPS-BioSePs against hydrogen peroxide-induced oxidative stress, as exhibited by cell viability reduction and suppression of ROS generation.
29050583	4	27	theme	generation	1422:1431	arg1	suppression					1403:1413	suppression	1403:1413	suppression of ROS generation	1403:1431	In vitro experiments with porcine jejunum epithelial (IPEC-J2) cells also indicated a significant cytoprotection of EPS-BioSePs against hydrogen peroxide-induced oxidative stress, as exhibited by cell viability reduction and suppression of ROS generation.
29050583	5	28	theme	appropriate	1612:1622	arg1	choice					1624:1629	an appropriate choice	1609:1629	an appropriate choice	1609:1629	These results suggested that this new form of selenium possessed great antioxidant property and cytoprotection and exopolysaccharide-producing bacteria could gradually become an appropriate choice to synthesize biogenic elemental selenium particles with potential applications as antioxidants.
29050583	2	29	theme	energy	585:590	arg1	EDX					623:625	EDX	623:625	EDX	623:625	Characterization by energy dispersive X-ray spectrometry (EDX), scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FT-IR), size measurement and chemical composition analysis verified that EPS-BioSePs exhibited a monodispersed and homogeneous spherical structure 293.73±4.03nm in size.
29050583	2	29	theme	energy	585:590	arg1	spectrometry					609:620	energy dispersive X-ray spectrometry	585:620	energy dispersive X-ray spectrometry (EDX)	585:626	Characterization by energy dispersive X-ray spectrometry (EDX), scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FT-IR), size measurement and chemical composition analysis verified that EPS-BioSePs exhibited a monodispersed and homogeneous spherical structure 293.73±4.03nm in size.
29050583	2	30	theme	size	794:797	arg1	measurement					799:809	size measurement	794:809	size measurement	794:809	Characterization by energy dispersive X-ray spectrometry (EDX), scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FT-IR), size measurement and chemical composition analysis verified that EPS-BioSePs exhibited a monodispersed and homogeneous spherical structure 293.73±4.03nm in size.
29050583	1	31	theme	selenium	232:239	arg1	CheSePs					252:258	CheSePs	252:258	CheSePs	252:258	Instead of using existing methods to chemically synthesize elemental selenium particles (CheSePs), which first require separating and purifying polysaccharides or proteins and adding extra reducing agent, this study applied a novel method to directly assemble exopolysaccharide-capped biogenic elemental selenium particles (EPS-BioSePs) by Bacillus paralicheniformis SR14 during the metabolic process.
29050583	1	31	theme	selenium	232:239	arg1	particles					241:249	elemental selenium particles	222:249	elemental selenium particles (CheSePs)	222:259	Instead of using existing methods to chemically synthesize elemental selenium particles (CheSePs), which first require separating and purifying polysaccharides or proteins and adding extra reducing agent, this study applied a novel method to directly assemble exopolysaccharide-capped biogenic elemental selenium particles (EPS-BioSePs) by Bacillus paralicheniformis SR14 during the metabolic process.
29050583	3	32	theme	hydroxyl	1160:1167	arg1	radical					1169:1175	hydroxyl radical	1160:1175	hydroxyl radical	1160:1175	Compared to a widely used form of CheSePs stabilized and coated by bovine serum albumin, EPS-BioSePs exhibited better antioxidant properties on scavenging DPPH, superoxide and ABTS free radicals, but not hydroxyl radical.
29050583	1	33	theme	metabolic	546:554	arg1	process					556:562	the metabolic process	542:562	the metabolic process	542:562	Instead of using existing methods to chemically synthesize elemental selenium particles (CheSePs), which first require separating and purifying polysaccharides or proteins and adding extra reducing agent, this study applied a novel method to directly assemble exopolysaccharide-capped biogenic elemental selenium particles (EPS-BioSePs) by Bacillus paralicheniformis SR14 during the metabolic process.
29050583	4	34	theme	viability	1379:1387	arg1	reduction					1389:1397	cell viability reduction	1374:1397	cell viability reduction	1374:1397	In vitro experiments with porcine jejunum epithelial (IPEC-J2) cells also indicated a significant cytoprotection of EPS-BioSePs against hydrogen peroxide-induced oxidative stress, as exhibited by cell viability reduction and suppression of ROS generation.
29050583	4	35	theme	hydrogen	1314:1321	arg1	stress					1350:1355	hydrogen peroxide-induced oxidative stress	1314:1355	hydrogen peroxide-induced oxidative stress	1314:1355	In vitro experiments with porcine jejunum epithelial (IPEC-J2) cells also indicated a significant cytoprotection of EPS-BioSePs against hydrogen peroxide-induced oxidative stress, as exhibited by cell viability reduction and suppression of ROS generation.
29050583	2	36	theme	scanning	629:636	arg1	microscopy					647:656	scanning electron microscopy	629:656	scanning electron microscopy (SEM)	629:662	Characterization by energy dispersive X-ray spectrometry (EDX), scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FT-IR), size measurement and chemical composition analysis verified that EPS-BioSePs exhibited a monodispersed and homogeneous spherical structure 293.73±4.03nm in size.
29050583	2	36	theme	scanning	629:636	arg1	SEM					659:661	SEM	659:661	SEM	659:661	Characterization by energy dispersive X-ray spectrometry (EDX), scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FT-IR), size measurement and chemical composition analysis verified that EPS-BioSePs exhibited a monodispersed and homogeneous spherical structure 293.73±4.03nm in size.
29050583	3	37	theme	superoxide	1117:1126	arg1	radicals					1142:1149	scavenging DPPH, superoxide and ABTS free radicals	1100:1149	radicals	1142:1149	Compared to a widely used form of CheSePs stabilized and coated by bovine serum albumin, EPS-BioSePs exhibited better antioxidant properties on scavenging DPPH, superoxide and ABTS free radicals, but not hydroxyl radical.
29050583	1	38	dep	require	274:280	arg1	adding					339:344	adding	339:344	adding extra reducing agent	339:365	Instead of using existing methods to chemically synthesize elemental selenium particles (CheSePs), which first require separating and purifying polysaccharides or proteins and adding extra reducing agent, this study applied a novel method to directly assemble exopolysaccharide-capped biogenic elemental selenium particles (EPS-BioSePs) by Bacillus paralicheniformis SR14 during the metabolic process.
29050583	1	38	dep	require	274:280	arg1	separating					282:291	separating	282:291	separating and purifying polysaccharides or proteins	282:333	Instead of using existing methods to chemically synthesize elemental selenium particles (CheSePs), which first require separating and purifying polysaccharides or proteins and adding extra reducing agent, this study applied a novel method to directly assemble exopolysaccharide-capped biogenic elemental selenium particles (EPS-BioSePs) by Bacillus paralicheniformis SR14 during the metabolic process.
29050583	2	39	dep	Fourier	745:751	arg1	transform					753:761	transform	753:761	transform infrared spectroscopy (FT-IR)	753:791	Characterization by energy dispersive X-ray spectrometry (EDX), scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FT-IR), size measurement and chemical composition analysis verified that EPS-BioSePs exhibited a monodispersed and homogeneous spherical structure 293.73±4.03nm in size.
29050583	0	40	theme	antioxidant	18:28	arg1	property					30:37	antioxidant property	18:37	antioxidant property	18:37	Characterization, antioxidant property and cytoprotection of exopolysaccharide-capped elemental selenium particles synthesized by Bacillus paralicheniformis SR14.
29050583	5	41	theme	great	1499:1503	arg1	property					1517:1524	great antioxidant property	1499:1524	great antioxidant property	1499:1524	These results suggested that this new form of selenium possessed great antioxidant property and cytoprotection and exopolysaccharide-producing bacteria could gradually become an appropriate choice to synthesize biogenic elemental selenium particles with potential applications as antioxidants.
29050583	1	42	theme	exopolysaccharide-capped	423:446	arg1	EPS-BioSePs					487:497	EPS-BioSePs	487:497	EPS-BioSePs	487:497	Instead of using existing methods to chemically synthesize elemental selenium particles (CheSePs), which first require separating and purifying polysaccharides or proteins and adding extra reducing agent, this study applied a novel method to directly assemble exopolysaccharide-capped biogenic elemental selenium particles (EPS-BioSePs) by Bacillus paralicheniformis SR14 during the metabolic process.
29050583	1	42	theme	exopolysaccharide-capped	423:446	arg1	particles					476:484	exopolysaccharide-capped biogenic elemental selenium particles	423:484	exopolysaccharide-capped biogenic elemental selenium particles (EPS-BioSePs)	423:498	Instead of using existing methods to chemically synthesize elemental selenium particles (CheSePs), which first require separating and purifying polysaccharides or proteins and adding extra reducing agent, this study applied a novel method to directly assemble exopolysaccharide-capped biogenic elemental selenium particles (EPS-BioSePs) by Bacillus paralicheniformis SR14 during the metabolic process.
29050583	5	43	theme	new	1468:1470	arg1	form					1472:1475	this new form	1463:1475	this new form of selenium	1463:1487	These results suggested that this new form of selenium possessed great antioxidant property and cytoprotection and exopolysaccharide-producing bacteria could gradually become an appropriate choice to synthesize biogenic elemental selenium particles with potential applications as antioxidants.
29050583	1	44	theme	extra	346:350	arg1	agent					361:365	extra reducing agent	346:365	extra reducing agent	346:365	Instead of using existing methods to chemically synthesize elemental selenium particles (CheSePs), which first require separating and purifying polysaccharides or proteins and adding extra reducing agent, this study applied a novel method to directly assemble exopolysaccharide-capped biogenic elemental selenium particles (EPS-BioSePs) by Bacillus paralicheniformis SR14 during the metabolic process.
29050583	1	45	theme	biogenic	448:455	arg1	EPS-BioSePs					487:497	EPS-BioSePs	487:497	EPS-BioSePs	487:497	Instead of using existing methods to chemically synthesize elemental selenium particles (CheSePs), which first require separating and purifying polysaccharides or proteins and adding extra reducing agent, this study applied a novel method to directly assemble exopolysaccharide-capped biogenic elemental selenium particles (EPS-BioSePs) by Bacillus paralicheniformis SR14 during the metabolic process.
29050583	1	45	theme	biogenic	448:455	arg1	particles					476:484	exopolysaccharide-capped biogenic elemental selenium particles	423:484	exopolysaccharide-capped biogenic elemental selenium particles (EPS-BioSePs)	423:498	Instead of using existing methods to chemically synthesize elemental selenium particles (CheSePs), which first require separating and purifying polysaccharides or proteins and adding extra reducing agent, this study applied a novel method to directly assemble exopolysaccharide-capped biogenic elemental selenium particles (EPS-BioSePs) by Bacillus paralicheniformis SR14 during the metabolic process.
29050583	2	46	theme	composition	824:834	arg1	analysis					836:843	chemical composition analysis	815:843	chemical composition analysis	815:843	Characterization by energy dispersive X-ray spectrometry (EDX), scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FT-IR), size measurement and chemical composition analysis verified that EPS-BioSePs exhibited a monodispersed and homogeneous spherical structure 293.73±4.03nm in size.
29050583	4	47	dep	In	1178:1179	arg1	vitro					1181:1185	vitro	1181:1185	vitro	1181:1185	In vitro experiments with porcine jejunum epithelial (IPEC-J2) cells also indicated a significant cytoprotection of EPS-BioSePs against hydrogen peroxide-induced oxidative stress, as exhibited by cell viability reduction and suppression of ROS generation.
29050583	1	48	theme	reducing	352:359	arg1	agent					361:365	extra reducing agent	346:365	extra reducing agent	346:365	Instead of using existing methods to chemically synthesize elemental selenium particles (CheSePs), which first require separating and purifying polysaccharides or proteins and adding extra reducing agent, this study applied a novel method to directly assemble exopolysaccharide-capped biogenic elemental selenium particles (EPS-BioSePs) by Bacillus paralicheniformis SR14 during the metabolic process.
29050583	5	49	theme	potential	1688:1696	arg1	applications					1698:1709	potential applications	1688:1709	potential applications as antioxidants	1688:1725	These results suggested that this new form of selenium possessed great antioxidant property and cytoprotection and exopolysaccharide-producing bacteria could gradually become an appropriate choice to synthesize biogenic elemental selenium particles with potential applications as antioxidants.
29050583	1	50	theme	elemental	457:465	arg1	EPS-BioSePs					487:497	EPS-BioSePs	487:497	EPS-BioSePs	487:497	Instead of using existing methods to chemically synthesize elemental selenium particles (CheSePs), which first require separating and purifying polysaccharides or proteins and adding extra reducing agent, this study applied a novel method to directly assemble exopolysaccharide-capped biogenic elemental selenium particles (EPS-BioSePs) by Bacillus paralicheniformis SR14 during the metabolic process.
29050583	1	50	theme	elemental	457:465	arg1	particles					476:484	exopolysaccharide-capped biogenic elemental selenium particles	423:484	exopolysaccharide-capped biogenic elemental selenium particles (EPS-BioSePs)	423:498	Instead of using existing methods to chemically synthesize elemental selenium particles (CheSePs), which first require separating and purifying polysaccharides or proteins and adding extra reducing agent, this study applied a novel method to directly assemble exopolysaccharide-capped biogenic elemental selenium particles (EPS-BioSePs) by Bacillus paralicheniformis SR14 during the metabolic process.
29050583	5	51	theme	selenium	1480:1487	arg1	form					1472:1475	this new form	1463:1475	this new form of selenium	1463:1487	These results suggested that this new form of selenium possessed great antioxidant property and cytoprotection and exopolysaccharide-producing bacteria could gradually become an appropriate choice to synthesize biogenic elemental selenium particles with potential applications as antioxidants.
29050583	2	52	theme	dispersive	592:601	arg1	EDX					623:625	EDX	623:625	EDX	623:625	Characterization by energy dispersive X-ray spectrometry (EDX), scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FT-IR), size measurement and chemical composition analysis verified that EPS-BioSePs exhibited a monodispersed and homogeneous spherical structure 293.73±4.03nm in size.
29050583	2	52	theme	dispersive	592:601	arg1	spectrometry					609:620	energy dispersive X-ray spectrometry	585:620	energy dispersive X-ray spectrometry (EDX)	585:626	Characterization by energy dispersive X-ray spectrometry (EDX), scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FT-IR), size measurement and chemical composition analysis verified that EPS-BioSePs exhibited a monodispersed and homogeneous spherical structure 293.73±4.03nm in size.
29050583	0	53	theme	exopolysaccharide-capped	61:84	arg1	selenium					96:103	exopolysaccharide-capped elemental selenium	61:103	exopolysaccharide-capped elemental selenium particles	61:113	Characterization, antioxidant property and cytoprotection of exopolysaccharide-capped elemental selenium particles synthesized by Bacillus paralicheniformis SR14.
29050583	4	54	theme	porcine	1204:1210	arg1	cells					1241:1245	porcine jejunum epithelial (IPEC-J2) cells	1204:1245	porcine jejunum epithelial (IPEC-J2) cells	1204:1245	In vitro experiments with porcine jejunum epithelial (IPEC-J2) cells also indicated a significant cytoprotection of EPS-BioSePs against hydrogen peroxide-induced oxidative stress, as exhibited by cell viability reduction and suppression of ROS generation.
29050583	5	55	theme	selenium	1664:1671	arg1	particles					1673:1681	biogenic elemental selenium particles	1645:1681	biogenic elemental selenium particles with potential applications as antioxidants	1645:1725	These results suggested that this new form of selenium possessed great antioxidant property and cytoprotection and exopolysaccharide-producing bacteria could gradually become an appropriate choice to synthesize biogenic elemental selenium particles with potential applications as antioxidants.
29050583	1	56	theme	selenium	467:474	arg1	EPS-BioSePs					487:497	EPS-BioSePs	487:497	EPS-BioSePs	487:497	Instead of using existing methods to chemically synthesize elemental selenium particles (CheSePs), which first require separating and purifying polysaccharides or proteins and adding extra reducing agent, this study applied a novel method to directly assemble exopolysaccharide-capped biogenic elemental selenium particles (EPS-BioSePs) by Bacillus paralicheniformis SR14 during the metabolic process.
29050583	1	56	theme	selenium	467:474	arg1	particles					476:484	exopolysaccharide-capped biogenic elemental selenium particles	423:484	exopolysaccharide-capped biogenic elemental selenium particles (EPS-BioSePs)	423:498	Instead of using existing methods to chemically synthesize elemental selenium particles (CheSePs), which first require separating and purifying polysaccharides or proteins and adding extra reducing agent, this study applied a novel method to directly assemble exopolysaccharide-capped biogenic elemental selenium particles (EPS-BioSePs) by Bacillus paralicheniformis SR14 during the metabolic process.
29050583	4	57	theme	IPEC-J2	1232:1238	arg1	cells					1241:1245	porcine jejunum epithelial (IPEC-J2) cells	1204:1245	porcine jejunum epithelial (IPEC-J2) cells	1204:1245	In vitro experiments with porcine jejunum epithelial (IPEC-J2) cells also indicated a significant cytoprotection of EPS-BioSePs against hydrogen peroxide-induced oxidative stress, as exhibited by cell viability reduction and suppression of ROS generation.
29050583	2	58	theme	infrared	763:770	arg1	FT-IR					786:790	FT-IR	786:790	FT-IR	786:790	Characterization by energy dispersive X-ray spectrometry (EDX), scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FT-IR), size measurement and chemical composition analysis verified that EPS-BioSePs exhibited a monodispersed and homogeneous spherical structure 293.73±4.03nm in size.
29050583	2	58	theme	infrared	763:770	arg1	spectroscopy					772:783	infrared spectroscopy	763:783	infrared spectroscopy (FT-IR)	763:791	Characterization by energy dispersive X-ray spectrometry (EDX), scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FT-IR), size measurement and chemical composition analysis verified that EPS-BioSePs exhibited a monodispersed and homogeneous spherical structure 293.73±4.03nm in size.
29050583	4	59	theme	significant	1264:1274	arg1	cytoprotection					1276:1289	a significant cytoprotection	1262:1289	a significant cytoprotection of EPS-BioSePs against hydrogen peroxide-induced oxidative stress	1262:1355	In vitro experiments with porcine jejunum epithelial (IPEC-J2) cells also indicated a significant cytoprotection of EPS-BioSePs against hydrogen peroxide-induced oxidative stress, as exhibited by cell viability reduction and suppression of ROS generation.
29050583	4	60	with	experiments	1187:1197	arg1	cells					1241:1245	porcine jejunum epithelial (IPEC-J2) cells	1204:1245	porcine jejunum epithelial (IPEC-J2) cells	1204:1245	In vitro experiments with porcine jejunum epithelial (IPEC-J2) cells also indicated a significant cytoprotection of EPS-BioSePs against hydrogen peroxide-induced oxidative stress, as exhibited by cell viability reduction and suppression of ROS generation.
29050583	3	61	theme	ABTS	1132:1135	arg1	radicals					1142:1149	scavenging DPPH, superoxide and ABTS free radicals	1100:1149	radicals	1142:1149	Compared to a widely used form of CheSePs stabilized and coated by bovine serum albumin, EPS-BioSePs exhibited better antioxidant properties on scavenging DPPH, superoxide and ABTS free radicals, but not hydroxyl radical.
29050583	4	62	theme	oxidative	1340:1348	arg1	stress					1350:1355	hydrogen peroxide-induced oxidative stress	1314:1355	hydrogen peroxide-induced oxidative stress	1314:1355	In vitro experiments with porcine jejunum epithelial (IPEC-J2) cells also indicated a significant cytoprotection of EPS-BioSePs against hydrogen peroxide-induced oxidative stress, as exhibited by cell viability reduction and suppression of ROS generation.
29050583	3	63	theme	bovine	1023:1028	arg1	albumin					1036:1042	bovine serum albumin	1023:1042	bovine serum albumin	1023:1042	Compared to a widely used form of CheSePs stabilized and coated by bovine serum albumin, EPS-BioSePs exhibited better antioxidant properties on scavenging DPPH, superoxide and ABTS free radicals, but not hydroxyl radical.
29050583	5	64	theme	biogenic	1645:1652	arg1	selenium					1664:1671	biogenic elemental selenium	1645:1671	biogenic elemental selenium particles with potential applications as antioxidants	1645:1725	These results suggested that this new form of selenium possessed great antioxidant property and cytoprotection and exopolysaccharide-producing bacteria could gradually become an appropriate choice to synthesize biogenic elemental selenium particles with potential applications as antioxidants.
29050583	2	65	theme	monodispersed	883:895	arg1	293.73±4.03nm					933:945	a monodispersed and homogeneous spherical structure 293.73±4.03nm	881:945	a monodispersed and homogeneous spherical structure 293.73±4.03nm	881:945	Characterization by energy dispersive X-ray spectrometry (EDX), scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FT-IR), size measurement and chemical composition analysis verified that EPS-BioSePs exhibited a monodispersed and homogeneous spherical structure 293.73±4.03nm in size.
29050583	2	66	theme	photoelectron	711:723	arg1	XPS					739:741	XPS	739:741	XPS	739:741	Characterization by energy dispersive X-ray spectrometry (EDX), scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FT-IR), size measurement and chemical composition analysis verified that EPS-BioSePs exhibited a monodispersed and homogeneous spherical structure 293.73±4.03nm in size.
29050583	2	66	theme	photoelectron	711:723	arg1	spectroscopy					725:736	X-ray photoelectron spectroscopy	705:736	X-ray photoelectron spectroscopy (XPS)	705:742	Characterization by energy dispersive X-ray spectrometry (EDX), scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FT-IR), size measurement and chemical composition analysis verified that EPS-BioSePs exhibited a monodispersed and homogeneous spherical structure 293.73±4.03nm in size.
29050583	5	67	contain	possessed	1489:1497	arg1	form					1472:1475	this new form	1463:1475	this new form of selenium	1463:1487	These results suggested that this new form of selenium possessed great antioxidant property and cytoprotection and exopolysaccharide-producing bacteria could gradually become an appropriate choice to synthesize biogenic elemental selenium particles with potential applications as antioxidants.
29050583	5	67	contain	possessed	1489:1497	arg2	property					1517:1524	great antioxidant property	1499:1524	great antioxidant property	1499:1524	These results suggested that this new form of selenium possessed great antioxidant property and cytoprotection and exopolysaccharide-producing bacteria could gradually become an appropriate choice to synthesize biogenic elemental selenium particles with potential applications as antioxidants.
29050583	2	68	theme	electron	638:645	arg1	microscopy					647:656	scanning electron microscopy	629:656	scanning electron microscopy (SEM)	629:662	Characterization by energy dispersive X-ray spectrometry (EDX), scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FT-IR), size measurement and chemical composition analysis verified that EPS-BioSePs exhibited a monodispersed and homogeneous spherical structure 293.73±4.03nm in size.
29050583	2	68	theme	electron	638:645	arg1	SEM					659:661	SEM	659:661	SEM	659:661	Characterization by energy dispersive X-ray spectrometry (EDX), scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FT-IR), size measurement and chemical composition analysis verified that EPS-BioSePs exhibited a monodispersed and homogeneous spherical structure 293.73±4.03nm in size.
29050583	4	69	theme	cell	1374:1377	arg1	reduction					1389:1397	cell viability reduction	1374:1397	cell viability reduction	1374:1397	In vitro experiments with porcine jejunum epithelial (IPEC-J2) cells also indicated a significant cytoprotection of EPS-BioSePs against hydrogen peroxide-induced oxidative stress, as exhibited by cell viability reduction and suppression of ROS generation.
29050583	3	70	theme	better	1067:1072	arg1	properties					1086:1095	better antioxidant properties	1067:1095	better antioxidant properties	1067:1095	Compared to a widely used form of CheSePs stabilized and coated by bovine serum albumin, EPS-BioSePs exhibited better antioxidant properties on scavenging DPPH, superoxide and ABTS free radicals, but not hydroxyl radical.
29050583	4	71	theme	In	1178:1179	arg1	experiments					1187:1197	In vitro experiments	1178:1197	In vitro experiments with porcine jejunum epithelial (IPEC-J2) cells	1178:1245	In vitro experiments with porcine jejunum epithelial (IPEC-J2) cells also indicated a significant cytoprotection of EPS-BioSePs against hydrogen peroxide-induced oxidative stress, as exhibited by cell viability reduction and suppression of ROS generation.
29050583	0	72	theme	selenium	96:103	arg1	particles					105:113	exopolysaccharide-capped elemental selenium particles	61:113	exopolysaccharide-capped elemental selenium particles	61:113	Characterization, antioxidant property and cytoprotection of exopolysaccharide-capped elemental selenium particles synthesized by Bacillus paralicheniformis SR14.
29050583	3	73	theme	DPPH	1111:1114	arg1	radicals					1142:1149	scavenging DPPH, superoxide and ABTS free radicals	1100:1149	radicals	1142:1149	Compared to a widely used form of CheSePs stabilized and coated by bovine serum albumin, EPS-BioSePs exhibited better antioxidant properties on scavenging DPPH, superoxide and ABTS free radicals, but not hydroxyl radical.
29050583	3	74	theme	antioxidant	1074:1084	arg1	properties					1086:1095	better antioxidant properties	1067:1095	better antioxidant properties	1067:1095	Compared to a widely used form of CheSePs stabilized and coated by bovine serum albumin, EPS-BioSePs exhibited better antioxidant properties on scavenging DPPH, superoxide and ABTS free radicals, but not hydroxyl radical.
29050583	1	75	theme	existing	180:187	arg1	methods					189:195	existing methods	180:195	existing methods	180:195	Instead of using existing methods to chemically synthesize elemental selenium particles (CheSePs), which first require separating and purifying polysaccharides or proteins and adding extra reducing agent, this study applied a novel method to directly assemble exopolysaccharide-capped biogenic elemental selenium particles (EPS-BioSePs) by Bacillus paralicheniformis SR14 during the metabolic process.
29050583	4	76	theme	peroxide-induced	1323:1338	arg1	stress					1350:1355	hydrogen peroxide-induced oxidative stress	1314:1355	hydrogen peroxide-induced oxidative stress	1314:1355	In vitro experiments with porcine jejunum epithelial (IPEC-J2) cells also indicated a significant cytoprotection of EPS-BioSePs against hydrogen peroxide-induced oxidative stress, as exhibited by cell viability reduction and suppression of ROS generation.
29050583	0	77	theme	elemental	86:94	arg1	selenium					96:103	exopolysaccharide-capped elemental selenium	61:103	exopolysaccharide-capped elemental selenium particles	61:113	Characterization, antioxidant property and cytoprotection of exopolysaccharide-capped elemental selenium particles synthesized by Bacillus paralicheniformis SR14.
29050583	5	78	theme	exopolysaccharide-producing	1549:1575	arg1	bacteria					1577:1584	exopolysaccharide-producing bacteria	1549:1584	exopolysaccharide-producing bacteria	1549:1584	These results suggested that this new form of selenium possessed great antioxidant property and cytoprotection and exopolysaccharide-producing bacteria could gradually become an appropriate choice to synthesize biogenic elemental selenium particles with potential applications as antioxidants.
29050583	2	79	theme	structure	923:931	arg1	293.73±4.03nm					933:945	a monodispersed and homogeneous spherical structure 293.73±4.03nm	881:945	a monodispersed and homogeneous spherical structure 293.73±4.03nm	881:945	Characterization by energy dispersive X-ray spectrometry (EDX), scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FT-IR), size measurement and chemical composition analysis verified that EPS-BioSePs exhibited a monodispersed and homogeneous spherical structure 293.73±4.03nm in size.
29050583	5	80	theme	antioxidant	1505:1515	arg1	property					1517:1524	great antioxidant property	1499:1524	great antioxidant property	1499:1524	These results suggested that this new form of selenium possessed great antioxidant property and cytoprotection and exopolysaccharide-producing bacteria could gradually become an appropriate choice to synthesize biogenic elemental selenium particles with potential applications as antioxidants.
28821112	8	0	theme	electrospun	889:899	arg1	HCs					901:903	electrospun HCs	889:903	electrospun HCs	889:903	The mechanism of electrospun HCs was suggested.
28821112	3	1	theme	fluid	457:461	arg1	jet					463:465	the Taylor cone and straight fluid jet	428:465	jet	463:465	Ethanol was used as sheath fluid during coaxial processes, and the effects of its flow rates on the Taylor cone and straight fluid jet were investigated.
28821112	0	2	theme	drug	100:103	arg1	dissolution					64:74	rapid dissolution	58:74	rapid dissolution of poorly water-soluble drug	58:103	Electrospun hypromellose-based hydrophilic composites for rapid dissolution of poorly water-soluble drug.
28821112	9	3	theme	drugs	1116:1120	arg1	delivery					1083:1090	the effective delivery	1069:1090	the effective delivery of poorly water-soluble drugs	1069:1120	By utilizing modified coaxial electrospinning and combinations of drug carriers, new types of HPMC-based HCs can provide an alternative approach for the effective delivery of poorly water-soluble drugs.
28821112	9	4	theme	new	1001:1003	arg1	types					1005:1009	new types	1001:1009	new types of HPMC-based HCs	1001:1027	By utilizing modified coaxial electrospinning and combinations of drug carriers, new types of HPMC-based HCs can provide an alternative approach for the effective delivery of poorly water-soluble drugs.
28821112	7	5	dep	better	840:845	arg1	the					836:838	the	836:838	the	836:838	The smaller the diameters of electrospun HCs were, the better their performances were.
28821112	7	6	theme	electrospun	814:824	arg1	HCs					826:828	electrospun HCs	814:828	electrospun HCs	814:828	The smaller the diameters of electrospun HCs were, the better their performances were.
28821112	4	7	theme	dissolution	544:554	arg1	rate					556:559	drug dissolution rate	539:559	drug dissolution rate	539:559	The morphology, component state, hydrophilicity, and drug dissolution rate of the HCs were characterized.
28821112	6	8	theme	faster	752:757	arg1	rate					715:718	the dissolution rate	699:718	the dissolution rate of electrospun HCs	699:737	However, the dissolution rate of electrospun HCs was 10 times faster than that of casting HCs.
28821112	6	8	theme	faster	752:757	arg1	times					746:750	10 times	743:750	10 times faster than that of casting HCs	743:782	However, the dissolution rate of electrospun HCs was 10 times faster than that of casting HCs.
28821112	4	9	theme	drug	539:542	arg1	rate					556:559	drug dissolution rate	539:559	drug dissolution rate	539:559	The morphology, component state, hydrophilicity, and drug dissolution rate of the HCs were characterized.
28821112	9	10	theme	HPMC-based	1014:1023	arg1	HCs					1025:1027	HPMC-based HCs	1014:1027	HPMC-based HCs	1014:1027	By utilizing modified coaxial electrospinning and combinations of drug carriers, new types of HPMC-based HCs can provide an alternative approach for the effective delivery of poorly water-soluble drugs.
28821112	1	11	theme	rapid	170:174	arg1	dissolution					176:186	rapid dissolution	170:186	rapid dissolution of ferulic acid (FA)	170:207	Hypromellose (HPMC)-based hydrophilic composites (HCs) used for rapid dissolution of ferulic acid (FA) were investigated.
28821112	9	12	theme	HCs	1025:1027	arg1	types					1005:1009	new types	1001:1009	new types of HPMC-based HCs	1001:1027	By utilizing modified coaxial electrospinning and combinations of drug carriers, new types of HPMC-based HCs can provide an alternative approach for the effective delivery of poorly water-soluble drugs.
28821112	3	13	used	used	344:347	arg2	Ethanol					332:338	Ethanol	332:338	Ethanol	332:338	Ethanol was used as sheath fluid during coaxial processes, and the effects of its flow rates on the Taylor cone and straight fluid jet were investigated.
28821112	3	13	used	used	344:347	arg2	fluid					359:363	sheath fluid	352:363	sheath fluid during coaxial processes	352:388	Ethanol was used as sheath fluid during coaxial processes, and the effects of its flow rates on the Taylor cone and straight fluid jet were investigated.
28821112	6	14	theme	HCs	735:737	arg1	rate					715:718	the dissolution rate	699:718	the dissolution rate of electrospun HCs	699:737	However, the dissolution rate of electrospun HCs was 10 times faster than that of casting HCs.
28821112	6	14	theme	HCs	735:737	arg1	times					746:750	10 times	743:750	10 times faster than that of casting HCs	743:782	However, the dissolution rate of electrospun HCs was 10 times faster than that of casting HCs.
28821112	1	15	theme	ferulic	191:197	arg1	FA					205:206	FA	205:206	FA	205:206	Hypromellose (HPMC)-based hydrophilic composites (HCs) used for rapid dissolution of ferulic acid (FA) were investigated.
28821112	1	15	theme	ferulic	191:197	arg1	acid					199:202	ferulic acid	191:202	ferulic acid (FA)	191:207	Hypromellose (HPMC)-based hydrophilic composites (HCs) used for rapid dissolution of ferulic acid (FA) were investigated.
28821112	4	16	theme	component	502:510	arg1	state					512:516	component state	502:516	component state	502:516	The morphology, component state, hydrophilicity, and drug dissolution rate of the HCs were characterized.
28821112	6	17	theme	electrospun	723:733	arg1	HCs					735:737	electrospun HCs	723:737	electrospun HCs	723:737	However, the dissolution rate of electrospun HCs was 10 times faster than that of casting HCs.
28821112	1	18	theme	acid	199:202	arg1	dissolution					176:186	rapid dissolution	170:186	rapid dissolution of ferulic acid (FA)	170:207	Hypromellose (HPMC)-based hydrophilic composites (HCs) used for rapid dissolution of ferulic acid (FA) were investigated.
28821112	3	19	theme	sheath	352:357	arg1	fluid					359:363	sheath fluid	352:363	sheath fluid during coaxial processes	352:388	Ethanol was used as sheath fluid during coaxial processes, and the effects of its flow rates on the Taylor cone and straight fluid jet were investigated.
28821112	3	19	theme	sheath	352:357	arg1	Ethanol					332:338	Ethanol	332:338	Ethanol	332:338	Ethanol was used as sheath fluid during coaxial processes, and the effects of its flow rates on the Taylor cone and straight fluid jet were investigated.
28821112	9	20	theme	effective	1073:1081	arg1	delivery					1083:1090	the effective delivery	1069:1090	the effective delivery of poorly water-soluble drugs	1069:1120	By utilizing modified coaxial electrospinning and combinations of drug carriers, new types of HPMC-based HCs can provide an alternative approach for the effective delivery of poorly water-soluble drugs.
28821112	9	21	theme	alternative	1044:1054	arg1	approach					1056:1063	an alternative approach	1041:1063	an alternative approach for the effective delivery of poorly water-soluble drugs	1041:1120	By utilizing modified coaxial electrospinning and combinations of drug carriers, new types of HPMC-based HCs can provide an alternative approach for the effective delivery of poorly water-soluble drugs.
28821112	0	22	theme	rapid	58:62	arg1	dissolution					64:74	rapid dissolution	58:74	rapid dissolution of poorly water-soluble drug	58:103	Electrospun hypromellose-based hydrophilic composites for rapid dissolution of poorly water-soluble drug.
28821112	3	23	theme	straight	448:455	arg1	jet					463:465	the Taylor cone and straight fluid jet	428:465	jet	463:465	Ethanol was used as sheath fluid during coaxial processes, and the effects of its flow rates on the Taylor cone and straight fluid jet were investigated.
28821112	2	24	theme	Electrospun	228:238	arg1	HCs					252:254	Electrospun and casting HCs	228:254	Electrospun and casting HCs	228:254	Electrospun and casting HCs were prepared from a solution containing HPMC, FA, and polyethylene glycol.
28821112	3	25	theme	flow	414:417	arg1	rates					419:423	its flow rates	410:423	its flow rates	410:423	Ethanol was used as sheath fluid during coaxial processes, and the effects of its flow rates on the Taylor cone and straight fluid jet were investigated.
28821112	9	26	theme	modified	933:940	arg1	electrospinning					950:964	modified coaxial electrospinning	933:964	modified coaxial electrospinning	933:964	By utilizing modified coaxial electrospinning and combinations of drug carriers, new types of HPMC-based HCs can provide an alternative approach for the effective delivery of poorly water-soluble drugs.
28821112	2	27	contain	containing	286:295	arg2	FA					303:304	FA	303:304	FA	303:304	Electrospun and casting HCs were prepared from a solution containing HPMC, FA, and polyethylene glycol.
28821112	2	27	contain	containing	286:295	arg2	HPMC					297:300	HPMC	297:300	HPMC	297:300	Electrospun and casting HCs were prepared from a solution containing HPMC, FA, and polyethylene glycol.
28821112	2	27	contain	containing	286:295	arg1	solution					277:284	a solution	275:284	a solution containing HPMC, FA, and polyethylene glycol	275:329	Electrospun and casting HCs were prepared from a solution containing HPMC, FA, and polyethylene glycol.
28821112	2	27	contain	containing	286:295	arg2	glycol					324:329	polyethylene glycol	311:329	polyethylene glycol	311:329	Electrospun and casting HCs were prepared from a solution containing HPMC, FA, and polyethylene glycol.
28821112	3	28	theme	rates	419:423	arg1	effects					399:405	the effects	395:405	the effects of its flow rates on the Taylor cone and straight fluid jet	395:465	Ethanol was used as sheath fluid during coaxial processes, and the effects of its flow rates on the Taylor cone and straight fluid jet were investigated.
28821112	3	29	theme	coaxial	372:378	arg1	processes					380:388	coaxial processes	372:388	coaxial processes	372:388	Ethanol was used as sheath fluid during coaxial processes, and the effects of its flow rates on the Taylor cone and straight fluid jet were investigated.
28821112	7	30	dep	smaller	789:795	arg1	diameters					801:809	the diameters	797:809	The smaller the diameters of electrospun HCs	785:828	The smaller the diameters of electrospun HCs were, the better their performances were.
28821112	1	31	theme	-based	125:130	arg1	composites					144:153	Hypromellose (HPMC)-based hydrophilic composites	106:153	Hypromellose (HPMC)-based hydrophilic composites (HCs) used for rapid dissolution of ferulic acid (FA)	106:207	Hypromellose (HPMC)-based hydrophilic composites (HCs) used for rapid dissolution of ferulic acid (FA) were investigated.
28821112	1	31	theme	-based	125:130	arg1	HCs					156:158	HCs	156:158	HCs	156:158	Hypromellose (HPMC)-based hydrophilic composites (HCs) used for rapid dissolution of ferulic acid (FA) were investigated.
28821112	5	32	theme	amorphous	631:639	arg1	materials					641:649	amorphous materials	631:649	amorphous materials	631:649	Results demonstrated that all HCs were amorphous materials, and their components were compatible.
28821112	6	33	theme	dissolution	703:713	arg1	rate					715:718	the dissolution rate	699:718	the dissolution rate of electrospun HCs	699:737	However, the dissolution rate of electrospun HCs was 10 times faster than that of casting HCs.
28821112	6	33	theme	dissolution	703:713	arg1	times					746:750	10 times	743:750	10 times faster than that of casting HCs	743:782	However, the dissolution rate of electrospun HCs was 10 times faster than that of casting HCs.
28821112	1	34	theme	hydrophilic	132:142	arg1	composites					144:153	Hypromellose (HPMC)-based hydrophilic composites	106:153	Hypromellose (HPMC)-based hydrophilic composites (HCs) used for rapid dissolution of ferulic acid (FA)	106:207	Hypromellose (HPMC)-based hydrophilic composites (HCs) used for rapid dissolution of ferulic acid (FA) were investigated.
28821112	1	34	theme	hydrophilic	132:142	arg1	HCs					156:158	HCs	156:158	HCs	156:158	Hypromellose (HPMC)-based hydrophilic composites (HCs) used for rapid dissolution of ferulic acid (FA) were investigated.
28821112	3	35	theme	Taylor	432:437	arg1	cone					439:442	the Taylor cone and straight fluid jet	428:465	cone	439:442	Ethanol was used as sheath fluid during coaxial processes, and the effects of its flow rates on the Taylor cone and straight fluid jet were investigated.
28821112	9	36	theme	water-soluble	1102:1114	arg1	drugs					1116:1120	poorly water-soluble drugs	1095:1120	poorly water-soluble drugs	1095:1120	By utilizing modified coaxial electrospinning and combinations of drug carriers, new types of HPMC-based HCs can provide an alternative approach for the effective delivery of poorly water-soluble drugs.
28821112	6	37	theme	casting	772:778	arg1	HCs					780:782	casting HCs	772:782	casting HCs	772:782	However, the dissolution rate of electrospun HCs was 10 times faster than that of casting HCs.
28821112	0	38	theme	water-soluble	86:98	arg1	drug					100:103	poorly water-soluble drug	79:103	poorly water-soluble drug	79:103	Electrospun hypromellose-based hydrophilic composites for rapid dissolution of poorly water-soluble drug.
28821112	2	39	theme	polyethylene	311:322	arg1	glycol					324:329	polyethylene glycol	311:329	polyethylene glycol	311:329	Electrospun and casting HCs were prepared from a solution containing HPMC, FA, and polyethylene glycol.
28821112	2	40	theme	casting	244:250	arg1	HCs					252:254	Electrospun and casting HCs	228:254	Electrospun and casting HCs	228:254	Electrospun and casting HCs were prepared from a solution containing HPMC, FA, and polyethylene glycol.
28821112	3	41	from	effects	399:405	arg1	jet					463:465	the Taylor cone and straight fluid jet	428:465	jet	463:465	Ethanol was used as sheath fluid during coaxial processes, and the effects of its flow rates on the Taylor cone and straight fluid jet were investigated.
28821112	3	41	from	effects	399:405	arg1	cone					439:442	the Taylor cone and straight fluid jet	428:465	cone	439:442	Ethanol was used as sheath fluid during coaxial processes, and the effects of its flow rates on the Taylor cone and straight fluid jet were investigated.
28821112	9	42	theme	drug	986:989	arg1	carriers					991:998	drug carriers	986:998	drug carriers	986:998	By utilizing modified coaxial electrospinning and combinations of drug carriers, new types of HPMC-based HCs can provide an alternative approach for the effective delivery of poorly water-soluble drugs.
28821112	4	43	theme	HCs	568:570	arg1	morphology					490:499	The morphology	486:499	The morphology	486:499	The morphology, component state, hydrophilicity, and drug dissolution rate of the HCs were characterized.
28821112	4	43	theme	HCs	568:570	arg1	state					512:516	component state	502:516	component state	502:516	The morphology, component state, hydrophilicity, and drug dissolution rate of the HCs were characterized.
28821112	4	43	theme	HCs	568:570	arg1	rate					556:559	drug dissolution rate	539:559	drug dissolution rate	539:559	The morphology, component state, hydrophilicity, and drug dissolution rate of the HCs were characterized.
28821112	4	43	theme	HCs	568:570	arg1	hydrophilicity					519:532	hydrophilicity	519:532	hydrophilicity	519:532	The morphology, component state, hydrophilicity, and drug dissolution rate of the HCs were characterized.
28821112	9	44	theme	coaxial	942:948	arg1	electrospinning					950:964	modified coaxial electrospinning	933:964	modified coaxial electrospinning	933:964	By utilizing modified coaxial electrospinning and combinations of drug carriers, new types of HPMC-based HCs can provide an alternative approach for the effective delivery of poorly water-soluble drugs.
28821112	0	45	theme	hydrophilic	31:41	arg1	composites					43:52	hydrophilic composites	31:52	hydrophilic composites for rapid dissolution of poorly water-soluble drug	31:103	Electrospun hypromellose-based hydrophilic composites for rapid dissolution of poorly water-soluble drug.
28821112	8	46	theme	HCs	901:903	arg1	mechanism					876:884	The mechanism	872:884	The mechanism of electrospun HCs	872:903	The mechanism of electrospun HCs was suggested.
28821112	9	47	theme	carriers	991:998	arg1	combinations					970:981	combinations	970:981	combinations of drug carriers	970:998	By utilizing modified coaxial electrospinning and combinations of drug carriers, new types of HPMC-based HCs can provide an alternative approach for the effective delivery of poorly water-soluble drugs.
28821112	9	47	theme	carriers	991:998	arg1	electrospinning					950:964	modified coaxial electrospinning	933:964	modified coaxial electrospinning	933:964	By utilizing modified coaxial electrospinning and combinations of drug carriers, new types of HPMC-based HCs can provide an alternative approach for the effective delivery of poorly water-soluble drugs.
25498702	8	0	theme	starch	1270:1275	arg1	structure					1285:1293	the rigid starch aerogel structure	1260:1293	the rigid starch aerogel structure	1260:1293	Release from starch aerogel microspheres was driven by dissolution, fitting the first-order kinetics due to the rigid starch aerogel structure, and showed different release rate constant (k1) depending on the drug (0.075 and 0.160 min(-1) for ketoprofen and benzoic acid, respectively).
25498702	7	1	theme	different	1047:1055	arg1	contribution					1066:1077	different relative contribution	1047:1077	different relative contribution of erosion and diffusion mechanisms depending on the matrix composition	1047:1149	Controlled drug release from pectin and alginate aerogel microspheres fitted Gallagher-Corrigan release model (R(2)>0.99 in both cases), with different relative contribution of erosion and diffusion mechanisms depending on the matrix composition.
25498702	1	2	theme	soluble	170:176	arg1	drugs					178:182	poorly water soluble drugs	157:182	poorly water soluble drugs for oral administration	157:206	Polysaccharide-based aerogels in the form of microspheres were investigated as carriers of poorly water soluble drugs for oral administration.
25498702	4	3	theme	matrix	604:609	arg1	structure					611:619	the aerogel matrix structure	592:619	the aerogel matrix structure	592:619	Amount of drug loaded depended on the aerogel matrix structure and composition and reached values up to 1.0×10(-3) and 1.7×10(-3) g/m(2) for ketoprofen and benzoic acid in starch microspheres.
25498702	3	4	from	starch	368:373	arg1	microspheres					350:361	Aerogel microspheres	342:361	Aerogel microspheres from starch, pectin and alginate	342:394	Aerogel microspheres from starch, pectin and alginate were loaded with ketoprofen (anti-inflammatory drug) and benzoic acid (used in the management of urea cycle disorders) via supercritical CO2-assisted adsorption.
25498702	7	5	theme	drug	916:919	arg1	release					921:927	Controlled drug release	905:927	Controlled drug release from pectin and alginate aerogel microspheres	905:973	Controlled drug release from pectin and alginate aerogel microspheres fitted Gallagher-Corrigan release model (R(2)>0.99 in both cases), with different relative contribution of erosion and diffusion mechanisms depending on the matrix composition.
25498702	1	6	theme	drugs	178:182	arg1	carriers					145:152	carriers	145:152	carriers of poorly water soluble drugs for oral administration	145:206	Polysaccharide-based aerogels in the form of microspheres were investigated as carriers of poorly water soluble drugs for oral administration.
25498702	1	6	theme	drugs	178:182	arg1	aerogels					87:94	Polysaccharide-based aerogels	66:94	Polysaccharide-based aerogels in the form of microspheres	66:122	Polysaccharide-based aerogels in the form of microspheres were investigated as carriers of poorly water soluble drugs for oral administration.
25498702	4	7	theme	aerogel	596:602	arg1	structure					611:619	the aerogel matrix structure	592:619	the aerogel matrix structure	592:619	Amount of drug loaded depended on the aerogel matrix structure and composition and reached values up to 1.0×10(-3) and 1.7×10(-3) g/m(2) for ketoprofen and benzoic acid in starch microspheres.
25498702	6	8	theme	pH	878:879	arg1	6.8					899:901	6.8	899:901	6.8	899:901	Release behavior was evaluated in different pH media (pH 1.2 and 6.8).
25498702	6	8	theme	pH	878:879	arg1	media					881:885	different pH media	868:885	different pH media (pH 1.2 and 6.8)	868:902	Release behavior was evaluated in different pH media (pH 1.2 and 6.8).
25498702	6	8	theme	pH	878:879	arg1	pH					888:889	pH 1.2 and 6.8	888:901	pH	888:889	Release behavior was evaluated in different pH media (pH 1.2 and 6.8).
25498702	8	9	theme	starch	1165:1170	arg1	microspheres					1180:1191	starch aerogel microspheres	1165:1191	starch aerogel microspheres	1165:1191	Release from starch aerogel microspheres was driven by dissolution, fitting the first-order kinetics due to the rigid starch aerogel structure, and showed different release rate constant (k1) depending on the drug (0.075 and 0.160 min(-1) for ketoprofen and benzoic acid, respectively).
25498702	1	10	theme	Polysaccharide-based	66:85	arg1	carriers					145:152	carriers	145:152	carriers of poorly water soluble drugs for oral administration	145:206	Polysaccharide-based aerogels in the form of microspheres were investigated as carriers of poorly water soluble drugs for oral administration.
25498702	1	10	theme	Polysaccharide-based	66:85	arg1	aerogels					87:94	Polysaccharide-based aerogels	66:94	Polysaccharide-based aerogels in the form of microspheres	66:122	Polysaccharide-based aerogels in the form of microspheres were investigated as carriers of poorly water soluble drugs for oral administration.
25498702	1	11	from	aerogels	87:94	arg1	form					103:106	the form	99:106	the form of microspheres	99:122	Polysaccharide-based aerogels in the form of microspheres were investigated as carriers of poorly water soluble drugs for oral administration.
25498702	7	12	dep	model	1009:1013	arg1	R					1016:1016	R(2)>0.99	1016:1024	R(2)>0.99 in both cases	1016:1038	Controlled drug release from pectin and alginate aerogel microspheres fitted Gallagher-Corrigan release model (R(2)>0.99 in both cases), with different relative contribution of erosion and diffusion mechanisms depending on the matrix composition.
25498702	6	13	theme	different	868:876	arg1	6.8					899:901	6.8	899:901	6.8	899:901	Release behavior was evaluated in different pH media (pH 1.2 and 6.8).
25498702	6	13	theme	different	868:876	arg1	media					881:885	different pH media	868:885	different pH media (pH 1.2 and 6.8)	868:902	Release behavior was evaluated in different pH media (pH 1.2 and 6.8).
25498702	6	13	theme	different	868:876	arg1	pH					888:889	pH 1.2 and 6.8	888:901	pH	888:889	Release behavior was evaluated in different pH media (pH 1.2 and 6.8).
25498702	7	14	theme	Gallagher-Corrigan	982:999	arg1	model					1009:1013	Gallagher-Corrigan release model	982:1013	Gallagher-Corrigan release model (R(2)>0.99 in both cases)	982:1039	Controlled drug release from pectin and alginate aerogel microspheres fitted Gallagher-Corrigan release model (R(2)>0.99 in both cases), with different relative contribution of erosion and diffusion mechanisms depending on the matrix composition.
25498702	8	15	theme	aerogel	1277:1283	arg1	structure					1285:1293	the rigid starch aerogel structure	1260:1293	the rigid starch aerogel structure	1260:1293	Release from starch aerogel microspheres was driven by dissolution, fitting the first-order kinetics due to the rigid starch aerogel structure, and showed different release rate constant (k1) depending on the drug (0.075 and 0.160 min(-1) for ketoprofen and benzoic acid, respectively).
25498702	8	16	theme	due	1253:1255	arg1	kinetics					1244:1251	the first-order kinetics	1228:1251	the first-order kinetics due to the rigid starch aerogel structure	1228:1293	Release from starch aerogel microspheres was driven by dissolution, fitting the first-order kinetics due to the rigid starch aerogel structure, and showed different release rate constant (k1) depending on the drug (0.075 and 0.160 min(-1) for ketoprofen and benzoic acid, respectively).
25498702	7	17	theme	Controlled	905:914	arg1	release					921:927	Controlled drug release	905:927	Controlled drug release from pectin and alginate aerogel microspheres	905:973	Controlled drug release from pectin and alginate aerogel microspheres fitted Gallagher-Corrigan release model (R(2)>0.99 in both cases), with different relative contribution of erosion and diffusion mechanisms depending on the matrix composition.
25498702	2	18	theme	loading	308:314	arg1	capacity					316:323	the enhanced drug loading capacity	290:323	the enhanced drug loading capacity of dry aerogels	290:339	These bio-based carriers may combine the biocompatibility of polysaccharides and the enhanced drug loading capacity of dry aerogels.
25498702	7	19	theme	matrix	1132:1137	arg1	composition					1139:1149	the matrix composition	1128:1149	the matrix composition	1128:1149	Controlled drug release from pectin and alginate aerogel microspheres fitted Gallagher-Corrigan release model (R(2)>0.99 in both cases), with different relative contribution of erosion and diffusion mechanisms depending on the matrix composition.
25498702	7	20	theme	erosion	1082:1088	arg1	contribution					1066:1077	different relative contribution	1047:1077	different relative contribution of erosion and diffusion mechanisms depending on the matrix composition	1047:1149	Controlled drug release from pectin and alginate aerogel microspheres fitted Gallagher-Corrigan release model (R(2)>0.99 in both cases), with different relative contribution of erosion and diffusion mechanisms depending on the matrix composition.
25498702	2	21	theme	drug	303:306	arg1	capacity					316:323	the enhanced drug loading capacity	290:323	the enhanced drug loading capacity of dry aerogels	290:339	These bio-based carriers may combine the biocompatibility of polysaccharides and the enhanced drug loading capacity of dry aerogels.
25498702	1	22	theme	oral	188:191	arg1	administration					193:206	oral administration	188:206	oral administration	188:206	Polysaccharide-based aerogels in the form of microspheres were investigated as carriers of poorly water soluble drugs for oral administration.
25498702	0	23	theme	aerogel	21:27	arg1	microspheres					29:40	Polysaccharide-based aerogel microspheres	0:40	Polysaccharide-based aerogel microspheres	0:40	Polysaccharide-based aerogel microspheres for oral drug delivery.
25498702	8	24	theme	release	1317:1323	arg1	rate					1325:1328	different release rate constant	1307:1337	different release rate constant (k1)	1307:1342	Release from starch aerogel microspheres was driven by dissolution, fitting the first-order kinetics due to the rigid starch aerogel structure, and showed different release rate constant (k1) depending on the drug (0.075 and 0.160 min(-1) for ketoprofen and benzoic acid, respectively).
25498702	8	24	theme	release	1317:1323	arg1	k1					1340:1341	k1	1340:1341	k1	1340:1341	Release from starch aerogel microspheres was driven by dissolution, fitting the first-order kinetics due to the rigid starch aerogel structure, and showed different release rate constant (k1) depending on the drug (0.075 and 0.160 min(-1) for ketoprofen and benzoic acid, respectively).
25498702	3	25	theme	benzoic	453:459	arg1	acid					461:464	benzoic acid	453:464	benzoic acid (used in the management of urea cycle disorders)	453:513	Aerogel microspheres from starch, pectin and alginate were loaded with ketoprofen (anti-inflammatory drug) and benzoic acid (used in the management of urea cycle disorders) via supercritical CO2-assisted adsorption.
25498702	0	26	theme	Polysaccharide-based	0:19	arg1	microspheres					29:40	Polysaccharide-based aerogel microspheres	0:40	Polysaccharide-based aerogel microspheres	0:40	Polysaccharide-based aerogel microspheres for oral drug delivery.
25498702	8	27	theme	different	1307:1315	arg1	rate					1325:1328	different release rate constant	1307:1337	different release rate constant (k1)	1307:1342	Release from starch aerogel microspheres was driven by dissolution, fitting the first-order kinetics due to the rigid starch aerogel structure, and showed different release rate constant (k1) depending on the drug (0.075 and 0.160 min(-1) for ketoprofen and benzoic acid, respectively).
25498702	8	27	theme	different	1307:1315	arg1	k1					1340:1341	k1	1340:1341	k1	1340:1341	Release from starch aerogel microspheres was driven by dissolution, fitting the first-order kinetics due to the rigid starch aerogel structure, and showed different release rate constant (k1) depending on the drug (0.075 and 0.160 min(-1) for ketoprofen and benzoic acid, respectively).
25498702	4	28	theme	starch	730:735	arg1	microspheres					737:748	starch microspheres	730:748	starch microspheres	730:748	Amount of drug loaded depended on the aerogel matrix structure and composition and reached values up to 1.0×10(-3) and 1.7×10(-3) g/m(2) for ketoprofen and benzoic acid in starch microspheres.
25498702	7	29	theme	relative	1057:1064	arg1	contribution					1066:1077	different relative contribution	1047:1077	different relative contribution of erosion and diffusion mechanisms depending on the matrix composition	1047:1149	Controlled drug release from pectin and alginate aerogel microspheres fitted Gallagher-Corrigan release model (R(2)>0.99 in both cases), with different relative contribution of erosion and diffusion mechanisms depending on the matrix composition.
25498702	7	30	theme	diffusion	1094:1102	arg1	mechanisms					1104:1113	diffusion mechanisms	1094:1113	diffusion mechanisms	1094:1113	Controlled drug release from pectin and alginate aerogel microspheres fitted Gallagher-Corrigan release model (R(2)>0.99 in both cases), with different relative contribution of erosion and diffusion mechanisms depending on the matrix composition.
25498702	2	31	theme	aerogels	332:339	arg1	biocompatibility					250:265	the biocompatibility	246:265	the biocompatibility of polysaccharides	246:284	These bio-based carriers may combine the biocompatibility of polysaccharides and the enhanced drug loading capacity of dry aerogels.
25498702	2	31	theme	aerogels	332:339	arg1	capacity					316:323	the enhanced drug loading capacity	290:323	the enhanced drug loading capacity of dry aerogels	290:339	These bio-based carriers may combine the biocompatibility of polysaccharides and the enhanced drug loading capacity of dry aerogels.
25498702	5	32	theme	amorphous	789:797	arg1	state					799:803	the amorphous state	785:803	the amorphous state in the aerogel microspheres	785:831	After impregnation, drugs were in the amorphous state in the aerogel microspheres.
25498702	5	33	from	state	799:803	arg1	microspheres					820:831	the aerogel microspheres	808:831	the aerogel microspheres	808:831	After impregnation, drugs were in the amorphous state in the aerogel microspheres.
25498702	3	34	from	pectin	376:381	arg1	microspheres					350:361	Aerogel microspheres	342:361	Aerogel microspheres from starch, pectin and alginate	342:394	Aerogel microspheres from starch, pectin and alginate were loaded with ketoprofen (anti-inflammatory drug) and benzoic acid (used in the management of urea cycle disorders) via supercritical CO2-assisted adsorption.
25498702	2	35	theme	dry	328:330	arg1	aerogels					332:339	dry aerogels	328:339	dry aerogels	328:339	These bio-based carriers may combine the biocompatibility of polysaccharides and the enhanced drug loading capacity of dry aerogels.
25498702	7	36	from	microspheres	962:973	arg1	release					921:927	Controlled drug release	905:927	Controlled drug release from pectin and alginate aerogel microspheres	905:973	Controlled drug release from pectin and alginate aerogel microspheres fitted Gallagher-Corrigan release model (R(2)>0.99 in both cases), with different relative contribution of erosion and diffusion mechanisms depending on the matrix composition.
25498702	0	37	theme	drug	51:54	arg1	delivery					56:63	oral drug delivery	46:63	oral drug delivery	46:63	Polysaccharide-based aerogel microspheres for oral drug delivery.
25498702	8	38	dep	drug	1361:1364	arg1	-1					1387:1388	-1	1387:1388	-1	1387:1388	Release from starch aerogel microspheres was driven by dissolution, fitting the first-order kinetics due to the rigid starch aerogel structure, and showed different release rate constant (k1) depending on the drug (0.075 and 0.160 min(-1) for ketoprofen and benzoic acid, respectively).
25498702	8	38	dep	drug	1361:1364	arg1	min					1383:1385	0.075 and 0.160 min	1367:1385	0.075 and 0.160 min(-1) for ketoprofen and benzoic acid	1367:1421	Release from starch aerogel microspheres was driven by dissolution, fitting the first-order kinetics due to the rigid starch aerogel structure, and showed different release rate constant (k1) depending on the drug (0.075 and 0.160 min(-1) for ketoprofen and benzoic acid, respectively).
25498702	6	39	theme	Release	834:840	arg1	behavior					842:849	Release behavior	834:849	Release behavior	834:849	Release behavior was evaluated in different pH media (pH 1.2 and 6.8).
25498702	8	40	theme	rigid	1264:1268	arg1	structure					1285:1293	the rigid starch aerogel structure	1260:1293	the rigid starch aerogel structure	1260:1293	Release from starch aerogel microspheres was driven by dissolution, fitting the first-order kinetics due to the rigid starch aerogel structure, and showed different release rate constant (k1) depending on the drug (0.075 and 0.160 min(-1) for ketoprofen and benzoic acid, respectively).
25498702	0	41	theme	oral	46:49	arg1	delivery					56:63	oral drug delivery	46:63	oral drug delivery	46:63	Polysaccharide-based aerogel microspheres for oral drug delivery.
25498702	3	42	from	alginate	387:394	arg1	microspheres					350:361	Aerogel microspheres	342:361	Aerogel microspheres from starch, pectin and alginate	342:394	Aerogel microspheres from starch, pectin and alginate were loaded with ketoprofen (anti-inflammatory drug) and benzoic acid (used in the management of urea cycle disorders) via supercritical CO2-assisted adsorption.
25498702	4	43	dep	1.0×10	662:667	arg1	up					656:657	up	656:657	up	656:657	Amount of drug loaded depended on the aerogel matrix structure and composition and reached values up to 1.0×10(-3) and 1.7×10(-3) g/m(2) for ketoprofen and benzoic acid in starch microspheres.
25498702	5	44	theme	aerogel	812:818	arg1	microspheres					820:831	the aerogel microspheres	808:831	the aerogel microspheres	808:831	After impregnation, drugs were in the amorphous state in the aerogel microspheres.
25498702	3	45	theme	anti-inflammatory	425:441	arg1	ketoprofen					413:422	ketoprofen	413:422	ketoprofen (anti-inflammatory drug)	413:447	Aerogel microspheres from starch, pectin and alginate were loaded with ketoprofen (anti-inflammatory drug) and benzoic acid (used in the management of urea cycle disorders) via supercritical CO2-assisted adsorption.
25498702	3	45	theme	anti-inflammatory	425:441	arg1	drug					443:446	anti-inflammatory drug	425:446	anti-inflammatory drug	425:446	Aerogel microspheres from starch, pectin and alginate were loaded with ketoprofen (anti-inflammatory drug) and benzoic acid (used in the management of urea cycle disorders) via supercritical CO2-assisted adsorption.
25498702	9	46	theme	tuning	1491:1496	arg1	loading					1503:1509	tuning drug loading	1491:1509	tuning drug loading	1491:1509	Overall, the results point out the possibilities of tuning drug loading and release by carefully choosing the polysaccharide used to prepare the aerogels.
25498702	9	47	theme	drug	1498:1501	arg1	loading					1503:1509	tuning drug loading	1491:1509	tuning drug loading	1491:1509	Overall, the results point out the possibilities of tuning drug loading and release by carefully choosing the polysaccharide used to prepare the aerogels.
25498702	7	48	theme	aerogel	954:960	arg1	microspheres					962:973	alginate aerogel microspheres	945:973	alginate aerogel microspheres	945:973	Controlled drug release from pectin and alginate aerogel microspheres fitted Gallagher-Corrigan release model (R(2)>0.99 in both cases), with different relative contribution of erosion and diffusion mechanisms depending on the matrix composition.
25498702	3	49	theme	disorders	504:512	arg1	management					479:488	the management	475:488	the management of urea cycle disorders	475:512	Aerogel microspheres from starch, pectin and alginate were loaded with ketoprofen (anti-inflammatory drug) and benzoic acid (used in the management of urea cycle disorders) via supercritical CO2-assisted adsorption.
25498702	8	50	theme	benzoic	1410:1416	arg1	acid					1418:1421	benzoic acid	1410:1421	benzoic acid	1410:1421	Release from starch aerogel microspheres was driven by dissolution, fitting the first-order kinetics due to the rigid starch aerogel structure, and showed different release rate constant (k1) depending on the drug (0.075 and 0.160 min(-1) for ketoprofen and benzoic acid, respectively).
25498702	4	51	theme	drug	568:571	arg1	drug					568:571	drug	568:571	drug loaded	568:578	Amount of drug loaded depended on the aerogel matrix structure and composition and reached values up to 1.0×10(-3) and 1.7×10(-3) g/m(2) for ketoprofen and benzoic acid in starch microspheres.
25498702	4	51	theme	drug	568:571	arg1	Amount					558:563	Amount	558:563	Amount of drug loaded	558:578	Amount of drug loaded depended on the aerogel matrix structure and composition and reached values up to 1.0×10(-3) and 1.7×10(-3) g/m(2) for ketoprofen and benzoic acid in starch microspheres.
25498702	3	52	theme	Aerogel	342:348	arg1	microspheres					350:361	Aerogel microspheres	342:361	Aerogel microspheres from starch, pectin and alginate	342:394	Aerogel microspheres from starch, pectin and alginate were loaded with ketoprofen (anti-inflammatory drug) and benzoic acid (used in the management of urea cycle disorders) via supercritical CO2-assisted adsorption.
25498702	9	53	theme	loading	1503:1509	arg1	possibilities					1474:1486	the possibilities	1470:1486	the possibilities of tuning drug loading	1470:1509	Overall, the results point out the possibilities of tuning drug loading and release by carefully choosing the polysaccharide used to prepare the aerogels.
25498702	3	54	theme	supercritical	519:531	arg1	adsorption					546:555	supercritical CO2-assisted adsorption	519:555	supercritical CO2-assisted adsorption	519:555	Aerogel microspheres from starch, pectin and alginate were loaded with ketoprofen (anti-inflammatory drug) and benzoic acid (used in the management of urea cycle disorders) via supercritical CO2-assisted adsorption.
25498702	8	55	theme	first-order	1232:1242	arg1	kinetics					1244:1251	the first-order kinetics	1228:1251	the first-order kinetics due to the rigid starch aerogel structure	1228:1293	Release from starch aerogel microspheres was driven by dissolution, fitting the first-order kinetics due to the rigid starch aerogel structure, and showed different release rate constant (k1) depending on the drug (0.075 and 0.160 min(-1) for ketoprofen and benzoic acid, respectively).
25498702	7	56	theme	release	1001:1007	arg1	model					1009:1013	Gallagher-Corrigan release model	982:1013	Gallagher-Corrigan release model (R(2)>0.99 in both cases)	982:1039	Controlled drug release from pectin and alginate aerogel microspheres fitted Gallagher-Corrigan release model (R(2)>0.99 in both cases), with different relative contribution of erosion and diffusion mechanisms depending on the matrix composition.
25498702	2	57	theme	enhanced	294:301	arg1	capacity					316:323	the enhanced drug loading capacity	290:323	the enhanced drug loading capacity of dry aerogels	290:339	These bio-based carriers may combine the biocompatibility of polysaccharides and the enhanced drug loading capacity of dry aerogels.
25498702	8	58	from	microspheres	1180:1191	arg1	Release					1152:1158	Release	1152:1158	Release from starch aerogel microspheres	1152:1191	Release from starch aerogel microspheres was driven by dissolution, fitting the first-order kinetics due to the rigid starch aerogel structure, and showed different release rate constant (k1) depending on the drug (0.075 and 0.160 min(-1) for ketoprofen and benzoic acid, respectively).
25498702	3	59	theme	urea	493:496	arg1	disorders					504:512	urea cycle disorders	493:512	urea cycle disorders	493:512	Aerogel microspheres from starch, pectin and alginate were loaded with ketoprofen (anti-inflammatory drug) and benzoic acid (used in the management of urea cycle disorders) via supercritical CO2-assisted adsorption.
25498702	1	60	theme	microspheres	111:122	arg1	form					103:106	the form	99:106	the form of microspheres	99:122	Polysaccharide-based aerogels in the form of microspheres were investigated as carriers of poorly water soluble drugs for oral administration.
25498702	2	61	theme	bio-based	215:223	arg1	carriers					225:232	These bio-based carriers	209:232	These bio-based carriers	209:232	These bio-based carriers may combine the biocompatibility of polysaccharides and the enhanced drug loading capacity of dry aerogels.
25498702	0	62	dep	microspheres	29:40	arg1	delivery					56:63	oral drug delivery	46:63	oral drug delivery	46:63	Polysaccharide-based aerogel microspheres for oral drug delivery.
25498702	4	63	theme	benzoic	714:720	arg1	acid					722:725	benzoic acid	714:725	benzoic acid	714:725	Amount of drug loaded depended on the aerogel matrix structure and composition and reached values up to 1.0×10(-3) and 1.7×10(-3) g/m(2) for ketoprofen and benzoic acid in starch microspheres.
25498702	3	64	theme	cycle	498:502	arg1	disorders					504:512	urea cycle disorders	493:512	urea cycle disorders	493:512	Aerogel microspheres from starch, pectin and alginate were loaded with ketoprofen (anti-inflammatory drug) and benzoic acid (used in the management of urea cycle disorders) via supercritical CO2-assisted adsorption.
25498702	8	65	theme	constant	1330:1337	arg1	rate					1325:1328	different release rate constant	1307:1337	different release rate constant (k1)	1307:1342	Release from starch aerogel microspheres was driven by dissolution, fitting the first-order kinetics due to the rigid starch aerogel structure, and showed different release rate constant (k1) depending on the drug (0.075 and 0.160 min(-1) for ketoprofen and benzoic acid, respectively).
25498702	8	65	theme	constant	1330:1337	arg1	k1					1340:1341	k1	1340:1341	k1	1340:1341	Release from starch aerogel microspheres was driven by dissolution, fitting the first-order kinetics due to the rigid starch aerogel structure, and showed different release rate constant (k1) depending on the drug (0.075 and 0.160 min(-1) for ketoprofen and benzoic acid, respectively).
25498702	7	66	theme	alginate	945:952	arg1	microspheres					962:973	alginate aerogel microspheres	945:973	alginate aerogel microspheres	945:973	Controlled drug release from pectin and alginate aerogel microspheres fitted Gallagher-Corrigan release model (R(2)>0.99 in both cases), with different relative contribution of erosion and diffusion mechanisms depending on the matrix composition.
25498702	3	67	theme	CO2-assisted	533:544	arg1	adsorption					546:555	supercritical CO2-assisted adsorption	519:555	supercritical CO2-assisted adsorption	519:555	Aerogel microspheres from starch, pectin and alginate were loaded with ketoprofen (anti-inflammatory drug) and benzoic acid (used in the management of urea cycle disorders) via supercritical CO2-assisted adsorption.
25498702	7	68	from	pectin	934:939	arg1	release					921:927	Controlled drug release	905:927	Controlled drug release from pectin and alginate aerogel microspheres	905:973	Controlled drug release from pectin and alginate aerogel microspheres fitted Gallagher-Corrigan release model (R(2)>0.99 in both cases), with different relative contribution of erosion and diffusion mechanisms depending on the matrix composition.
25498702	8	69	theme	aerogel	1172:1178	arg1	microspheres					1180:1191	starch aerogel microspheres	1165:1191	starch aerogel microspheres	1165:1191	Release from starch aerogel microspheres was driven by dissolution, fitting the first-order kinetics due to the rigid starch aerogel structure, and showed different release rate constant (k1) depending on the drug (0.075 and 0.160 min(-1) for ketoprofen and benzoic acid, respectively).
25498702	7	70	from	contribution	1066:1077	arg1	composition					1139:1149	the matrix composition	1128:1149	the matrix composition	1128:1149	Controlled drug release from pectin and alginate aerogel microspheres fitted Gallagher-Corrigan release model (R(2)>0.99 in both cases), with different relative contribution of erosion and diffusion mechanisms depending on the matrix composition.
25498702	2	71	theme	polysaccharides	270:284	arg1	biocompatibility					250:265	the biocompatibility	246:265	the biocompatibility of polysaccharides	246:284	These bio-based carriers may combine the biocompatibility of polysaccharides and the enhanced drug loading capacity of dry aerogels.
25498702	2	71	theme	polysaccharides	270:284	arg1	capacity					316:323	the enhanced drug loading capacity	290:323	the enhanced drug loading capacity of dry aerogels	290:339	These bio-based carriers may combine the biocompatibility of polysaccharides and the enhanced drug loading capacity of dry aerogels.
25498702	7	72	theme	mechanisms	1104:1113	arg1	contribution					1066:1077	different relative contribution	1047:1077	different relative contribution of erosion and diffusion mechanisms depending on the matrix composition	1047:1149	Controlled drug release from pectin and alginate aerogel microspheres fitted Gallagher-Corrigan release model (R(2)>0.99 in both cases), with different relative contribution of erosion and diffusion mechanisms depending on the matrix composition.
25498702	7	73	from	R	1016:1016	arg1	cases					1034:1038	both cases	1029:1038	both cases	1029:1038	Controlled drug release from pectin and alginate aerogel microspheres fitted Gallagher-Corrigan release model (R(2)>0.99 in both cases), with different relative contribution of erosion and diffusion mechanisms depending on the matrix composition.
24276540	1	0	theme	gold	217:220	arg1	GNPs					237:240	GNPs	237:240	GNPs	237:240	For the soil nitrogen-fixing bacterium Azospirillum brasilense, the ability to reduce [AuCl4](-) and to form gold nanoparticles (GNPs) has been demonstrated, with the appearance of a mauve tint of the culture.
24276540	1	0	theme	gold	217:220	arg1	nanoparticles					222:234	gold nanoparticles	217:234	gold nanoparticles (GNPs)	217:241	For the soil nitrogen-fixing bacterium Azospirillum brasilense, the ability to reduce [AuCl4](-) and to form gold nanoparticles (GNPs) has been demonstrated, with the appearance of a mauve tint of the culture.
24276540	3	1	theme	studied	476:482	arg1	Sp7					541:543	Sp7	541:543	Sp7	541:543	For the widely studied agriculturally important wild-type strains A. brasilense Sp7 and Sp245, GNPs formed after 10 days of incubation of cell biomass with 0.25 mM [AuCl4](-) were shown (using TEM) to be mainly of spherical form (5 to 20 nm in diameter), with rare occasional triangles.
24276540	3	1	theme	studied	476:482	arg1	Sp245					549:553	Sp245	549:553	Sp245	549:553	For the widely studied agriculturally important wild-type strains A. brasilense Sp7 and Sp245, GNPs formed after 10 days of incubation of cell biomass with 0.25 mM [AuCl4](-) were shown (using TEM) to be mainly of spherical form (5 to 20 nm in diameter), with rare occasional triangles.
24276540	3	1	theme	studied	476:482	arg1	strains					519:525	the widely studied agriculturally important wild-type strains	465:525	the widely studied agriculturally important wild-type strains A. brasilense Sp7 and Sp245	465:553	For the widely studied agriculturally important wild-type strains A. brasilense Sp7 and Sp245, GNPs formed after 10 days of incubation of cell biomass with 0.25 mM [AuCl4](-) were shown (using TEM) to be mainly of spherical form (5 to 20 nm in diameter), with rare occasional triangles.
24276540	9	2	theme	use	1872:1874	arg1	synthesis					1832:1840	environmentally friendly synthesis	1807:1840	environmentally friendly synthesis of nanoparticles and possible use of bacterial cells with modified surface structure and composition for their fabrication	1807:1963	The data obtained may also help in developing protocols for environmentally friendly synthesis of nanoparticles and possible use of bacterial cells with modified surface structure and composition for their fabrication.
24276540	5	3	from	differences	979:989	arg1	structure					998:1006	structure	998:1006	structure	998:1006	Thus, for the mutant strain Sp245.5 (which has significant differences in the structure and composition of cell-surface polysaccharides as compared with Sp245), a more rapid formation of GNPs was observed.
24276540	5	3	from	differences	979:989	arg1	composition					1012:1022	composition	1012:1022	composition	1012:1022	Thus, for the mutant strain Sp245.5 (which has significant differences in the structure and composition of cell-surface polysaccharides as compared with Sp245), a more rapid formation of GNPs was observed.
24276540	0	4	theme	Gold	0:3	arg1	reduction					10:18	Gold(III) reduction	0:18	Gold(III) reduction by the rhizobacterium Azospirillum	0:53	Gold(III) reduction by the rhizobacterium Azospirillum brasilense with the formation of gold nanoparticles.
24276540	9	5	theme	bacterial	1879:1887	arg1	cells					1889:1893	bacterial cells	1879:1893	bacterial cells with modified surface structure and composition	1879:1941	The data obtained may also help in developing protocols for environmentally friendly synthesis of nanoparticles and possible use of bacterial cells with modified surface structure and composition for their fabrication.
24276540	6	6	theme	round-shaped	1276:1287	arg1	nanoparticles					1301:1313	larger round-shaped flower-like nanoparticles	1269:1313	larger round-shaped flower-like nanoparticles about 100 nm in size	1269:1334	Moreover, their TEM images (also obtained after 10 days) showed different shapes: nano-sized spheres, triangles, hexagons and rods, as well as larger round-shaped flower-like nanoparticles about 100 nm in size.
24276540	8	7	theme	formation	1703:1711	arg1	mechanisms					1670:1679	the natural biogeochemical mechanisms	1643:1679	the natural biogeochemical mechanisms of gold reduction and formation of GNPs involving microorganisms	1643:1744	This finding may be useful for understanding the natural biogeochemical mechanisms of gold reduction and formation of GNPs involving microorganisms.
24276540	7	8	theme	chemical	1502:1509	arg1	composition					1511:1521	chemical composition	1502:1521	chemical composition	1502:1521	Since by the time of GNP formation in our experiments the cells were found to be already not viable, this confirms the dominating role of cell surface structure and chemical composition in shaping the GNPs formed in the course of [AuCl4](-) reduction to Au(0).
24276540	3	9	theme	important	499:507	arg1	Sp7					541:543	Sp7	541:543	Sp7	541:543	For the widely studied agriculturally important wild-type strains A. brasilense Sp7 and Sp245, GNPs formed after 10 days of incubation of cell biomass with 0.25 mM [AuCl4](-) were shown (using TEM) to be mainly of spherical form (5 to 20 nm in diameter), with rare occasional triangles.
24276540	3	9	theme	important	499:507	arg1	Sp245					549:553	Sp245	549:553	Sp245	549:553	For the widely studied agriculturally important wild-type strains A. brasilense Sp7 and Sp245, GNPs formed after 10 days of incubation of cell biomass with 0.25 mM [AuCl4](-) were shown (using TEM) to be mainly of spherical form (5 to 20 nm in diameter), with rare occasional triangles.
24276540	3	9	theme	important	499:507	arg1	strains					519:525	the widely studied agriculturally important wild-type strains	465:525	the widely studied agriculturally important wild-type strains A. brasilense Sp7 and Sp245	465:553	For the widely studied agriculturally important wild-type strains A. brasilense Sp7 and Sp245, GNPs formed after 10 days of incubation of cell biomass with 0.25 mM [AuCl4](-) were shown (using TEM) to be mainly of spherical form (5 to 20 nm in diameter), with rare occasional triangles.
24276540	5	10	contain	has	963:965	arg1	Sp245.5					948:954	the mutant strain Sp245.5	930:954	the mutant strain Sp245.5 (which has significant differences in the structure and composition of cell-surface polysaccharides as compared with Sp245)	930:1078	Thus, for the mutant strain Sp245.5 (which has significant differences in the structure and composition of cell-surface polysaccharides as compared with Sp245), a more rapid formation of GNPs was observed.
24276540	5	10	contain	has	963:965	arg2	differences					979:989	significant differences	967:989	significant differences in the structure and composition of cell-surface polysaccharides	967:1054	Thus, for the mutant strain Sp245.5 (which has significant differences in the structure and composition of cell-surface polysaccharides as compared with Sp245), a more rapid formation of GNPs was observed.
24276540	9	11	theme	surface	1909:1915	arg1	structure					1917:1925	modified surface structure	1900:1925	modified surface structure	1900:1925	The data obtained may also help in developing protocols for environmentally friendly synthesis of nanoparticles and possible use of bacterial cells with modified surface structure and composition for their fabrication.
24276540	6	12	from	spheres	1219:1225	arg1	size					1331:1334	size	1331:1334	size	1331:1334	Moreover, their TEM images (also obtained after 10 days) showed different shapes: nano-sized spheres, triangles, hexagons and rods, as well as larger round-shaped flower-like nanoparticles about 100 nm in size.
24276540	4	13	theme	strain	854:859	arg1	cells					845:849	cells	845:849	cells of strain Sp245.5	845:867	In the course of cultivation with [AuCl4](-), after 5 days, a mauve tint was already visible for cells of strain Sp245.5, after 6 days for Sp245 and after 10 days for Sp7.
24276540	7	14	theme	surface	1480:1486	arg1	structure					1488:1496	cell surface structure	1475:1496	cell surface structure	1475:1496	Since by the time of GNP formation in our experiments the cells were found to be already not viable, this confirms the dominating role of cell surface structure and chemical composition in shaping the GNPs formed in the course of [AuCl4](-) reduction to Au(0).
24276540	7	15	theme	formation	1362:1370	arg1	time					1350:1353	the time	1346:1353	the time of GNP formation in our experiments	1346:1389	Since by the time of GNP formation in our experiments the cells were found to be already not viable, this confirms the dominating role of cell surface structure and chemical composition in shaping the GNPs formed in the course of [AuCl4](-) reduction to Au(0).
24276540	4	16	theme	cultivation	765:775	arg1	course					755:760	the course	751:760	the course of cultivation with [AuCl4](-)	751:791	In the course of cultivation with [AuCl4](-), after 5 days, a mauve tint was already visible for cells of strain Sp245.5, after 6 days for Sp245 and after 10 days for Sp7.
24276540	4	17	from	course	755:760	arg1	tint					816:819	a mauve tint	808:819	a mauve tint	808:819	In the course of cultivation with [AuCl4](-), after 5 days, a mauve tint was already visible for cells of strain Sp245.5, after 6 days for Sp245 and after 10 days for Sp7.
24276540	4	17	from	course	755:760	arg1	visible					833:839	visible	833:839	visible	833:839	In the course of cultivation with [AuCl4](-), after 5 days, a mauve tint was already visible for cells of strain Sp245.5, after 6 days for Sp245 and after 10 days for Sp7.
24276540	1	18	theme	soil	116:119	arg1	bacterium					137:145	soil nitrogen-fixing bacterium	116:145	the soil nitrogen-fixing bacterium Azospirillum brasilense	112:169	For the soil nitrogen-fixing bacterium Azospirillum brasilense, the ability to reduce [AuCl4](-) and to form gold nanoparticles (GNPs) has been demonstrated, with the appearance of a mauve tint of the culture.
24276540	2	19	theme	fluorescence	428:439	arg1	analysis					441:448	X-ray fluorescence analysis	422:448	X-ray fluorescence analysis	422:448	To validate the shapes and chemical nature of nanoparticles, transmission electron microscopy (TEM) and X-ray fluorescence analysis were used.
24276540	3	20	theme	rare	721:724	arg1	triangles					737:745	rare occasional triangles	721:745	rare occasional triangles	721:745	For the widely studied agriculturally important wild-type strains A. brasilense Sp7 and Sp245, GNPs formed after 10 days of incubation of cell biomass with 0.25 mM [AuCl4](-) were shown (using TEM) to be mainly of spherical form (5 to 20 nm in diameter), with rare occasional triangles.
24276540	3	21	theme	spherical	675:683	arg1	form					685:688	spherical form	675:688	spherical form (5 to 20 nm in diameter)	675:713	For the widely studied agriculturally important wild-type strains A. brasilense Sp7 and Sp245, GNPs formed after 10 days of incubation of cell biomass with 0.25 mM [AuCl4](-) were shown (using TEM) to be mainly of spherical form (5 to 20 nm in diameter), with rare occasional triangles.
24276540	8	22	theme	natural	1647:1653	arg1	mechanisms					1670:1679	the natural biogeochemical mechanisms	1643:1679	the natural biogeochemical mechanisms of gold reduction and formation of GNPs involving microorganisms	1643:1744	This finding may be useful for understanding the natural biogeochemical mechanisms of gold reduction and formation of GNPs involving microorganisms.
24276540	1	23	theme	bacterium	137:145	arg1	brasilense					160:169	the soil nitrogen-fixing bacterium Azospirillum brasilense	112:169	the soil nitrogen-fixing bacterium Azospirillum brasilense	112:169	For the soil nitrogen-fixing bacterium Azospirillum brasilense, the ability to reduce [AuCl4](-) and to form gold nanoparticles (GNPs) has been demonstrated, with the appearance of a mauve tint of the culture.
24276540	7	24	theme	dominating	1456:1465	arg1	role					1467:1470	the dominating role	1452:1470	the dominating role of cell surface structure and chemical composition in shaping the GNPs formed in the course of [AuCl4](-) reduction to Au(0)	1452:1595	Since by the time of GNP formation in our experiments the cells were found to be already not viable, this confirms the dominating role of cell surface structure and chemical composition in shaping the GNPs formed in the course of [AuCl4](-) reduction to Au(0).
24276540	4	25	theme	mauve	810:814	arg1	tint					816:819	a mauve tint	808:819	a mauve tint	808:819	In the course of cultivation with [AuCl4](-), after 5 days, a mauve tint was already visible for cells of strain Sp245.5, after 6 days for Sp245 and after 10 days for Sp7.
24276540	4	25	theme	mauve	810:814	arg1	visible					833:839	visible	833:839	visible	833:839	In the course of cultivation with [AuCl4](-), after 5 days, a mauve tint was already visible for cells of strain Sp245.5, after 6 days for Sp245 and after 10 days for Sp7.
24276540	7	26	from	formation	1362:1370	arg1	experiments					1379:1389	our experiments	1375:1389	our experiments	1375:1389	Since by the time of GNP formation in our experiments the cells were found to be already not viable, this confirms the dominating role of cell surface structure and chemical composition in shaping the GNPs formed in the course of [AuCl4](-) reduction to Au(0).
24276540	3	27	dep	strains	519:525	arg1	Sp7					541:543	Sp7	541:543	Sp7	541:543	For the widely studied agriculturally important wild-type strains A. brasilense Sp7 and Sp245, GNPs formed after 10 days of incubation of cell biomass with 0.25 mM [AuCl4](-) were shown (using TEM) to be mainly of spherical form (5 to 20 nm in diameter), with rare occasional triangles.
24276540	3	27	dep	strains	519:525	arg1	Sp245					549:553	Sp245	549:553	Sp245	549:553	For the widely studied agriculturally important wild-type strains A. brasilense Sp7 and Sp245, GNPs formed after 10 days of incubation of cell biomass with 0.25 mM [AuCl4](-) were shown (using TEM) to be mainly of spherical form (5 to 20 nm in diameter), with rare occasional triangles.
24276540	3	27	dep	strains	519:525	arg1	strains					519:525	the widely studied agriculturally important wild-type strains	465:525	the widely studied agriculturally important wild-type strains A. brasilense Sp7 and Sp245	465:553	For the widely studied agriculturally important wild-type strains A. brasilense Sp7 and Sp245, GNPs formed after 10 days of incubation of cell biomass with 0.25 mM [AuCl4](-) were shown (using TEM) to be mainly of spherical form (5 to 20 nm in diameter), with rare occasional triangles.
24276540	3	28	theme	occasional	726:735	arg1	triangles					737:745	rare occasional triangles	721:745	rare occasional triangles	721:745	For the widely studied agriculturally important wild-type strains A. brasilense Sp7 and Sp245, GNPs formed after 10 days of incubation of cell biomass with 0.25 mM [AuCl4](-) were shown (using TEM) to be mainly of spherical form (5 to 20 nm in diameter), with rare occasional triangles.
24276540	6	29	theme	TEM	1142:1144	arg1	images					1146:1151	their TEM images	1136:1151	their TEM images (also obtained after 10 days)	1136:1181	Moreover, their TEM images (also obtained after 10 days) showed different shapes: nano-sized spheres, triangles, hexagons and rods, as well as larger round-shaped flower-like nanoparticles about 100 nm in size.
24276540	1	30	theme	tint	297:300	arg1	appearance					275:284	the appearance	271:284	the appearance of a mauve tint of the culture	271:315	For the soil nitrogen-fixing bacterium Azospirillum brasilense, the ability to reduce [AuCl4](-) and to form gold nanoparticles (GNPs) has been demonstrated, with the appearance of a mauve tint of the culture.
24276540	0	31	theme	gold	88:91	arg1	nanoparticles					93:105	gold nanoparticles	88:105	gold nanoparticles	88:105	Gold(III) reduction by the rhizobacterium Azospirillum brasilense with the formation of gold nanoparticles.
24276540	2	32	theme	transmission	379:390	arg1	microscopy					401:410	transmission electron microscopy	379:410	transmission electron microscopy (TEM)	379:416	To validate the shapes and chemical nature of nanoparticles, transmission electron microscopy (TEM) and X-ray fluorescence analysis were used.
24276540	2	32	theme	transmission	379:390	arg1	TEM					413:415	TEM	413:415	TEM	413:415	To validate the shapes and chemical nature of nanoparticles, transmission electron microscopy (TEM) and X-ray fluorescence analysis were used.
24276540	6	33	theme	nano-sized	1208:1217	arg1	spheres					1219:1225	nano-sized spheres	1208:1225	nano-sized spheres	1208:1225	Moreover, their TEM images (also obtained after 10 days) showed different shapes: nano-sized spheres, triangles, hexagons and rods, as well as larger round-shaped flower-like nanoparticles about 100 nm in size.
24276540	6	34	dep	obtained	1159:1166	arg1	also					1154:1157	also	1154:1157	also	1154:1157	Moreover, their TEM images (also obtained after 10 days) showed different shapes: nano-sized spheres, triangles, hexagons and rods, as well as larger round-shaped flower-like nanoparticles about 100 nm in size.
24276540	2	35	theme	nanoparticles	364:376	arg1	shapes					334:339	shapes	334:339	shapes	334:339	To validate the shapes and chemical nature of nanoparticles, transmission electron microscopy (TEM) and X-ray fluorescence analysis were used.
24276540	2	35	theme	nanoparticles	364:376	arg1	nature					354:359	chemical nature	345:359	chemical nature	345:359	To validate the shapes and chemical nature of nanoparticles, transmission electron microscopy (TEM) and X-ray fluorescence analysis were used.
24276540	9	36	with	cells	1889:1893	arg1	structure					1917:1925	modified surface structure	1900:1925	modified surface structure	1900:1925	The data obtained may also help in developing protocols for environmentally friendly synthesis of nanoparticles and possible use of bacterial cells with modified surface structure and composition for their fabrication.
24276540	9	36	with	cells	1889:1893	arg1	composition					1931:1941	composition	1931:1941	composition	1931:1941	The data obtained may also help in developing protocols for environmentally friendly synthesis of nanoparticles and possible use of bacterial cells with modified surface structure and composition for their fabrication.
24276540	5	37	theme	significant	967:977	arg1	differences					979:989	significant differences	967:989	significant differences in the structure and composition of cell-surface polysaccharides	967:1054	Thus, for the mutant strain Sp245.5 (which has significant differences in the structure and composition of cell-surface polysaccharides as compared with Sp245), a more rapid formation of GNPs was observed.
24276540	4	38	with	course	755:760	arg1	[AuCl4					782:787	[AuCl4]	782:788	[AuCl4](-)	782:791	In the course of cultivation with [AuCl4](-), after 5 days, a mauve tint was already visible for cells of strain Sp245.5, after 6 days for Sp245 and after 10 days for Sp7.
24276540	3	39	theme	incubation	585:594	arg1	10 days					574:580	10 days	574:580	10 days of incubation of cell biomass with 0.25 mM [AuCl4](-)	574:634	For the widely studied agriculturally important wild-type strains A. brasilense Sp7 and Sp245, GNPs formed after 10 days of incubation of cell biomass with 0.25 mM [AuCl4](-) were shown (using TEM) to be mainly of spherical form (5 to 20 nm in diameter), with rare occasional triangles.
24276540	1	40	dep	ability	176:182	arg1	reduce					187:192	reduce	187:192	to reduce [AuCl4](-)	184:203	For the soil nitrogen-fixing bacterium Azospirillum brasilense, the ability to reduce [AuCl4](-) and to form gold nanoparticles (GNPs) has been demonstrated, with the appearance of a mauve tint of the culture.
24276540	1	40	dep	ability	176:182	arg1	form					212:215	form	212:215	to form gold nanoparticles (GNPs)	209:241	For the soil nitrogen-fixing bacterium Azospirillum brasilense, the ability to reduce [AuCl4](-) and to form gold nanoparticles (GNPs) has been demonstrated, with the appearance of a mauve tint of the culture.
24276540	5	41	theme	GNPs	1107:1110	arg1	formation					1094:1102	a more rapid formation	1081:1102	a more rapid formation of GNPs	1081:1110	Thus, for the mutant strain Sp245.5 (which has significant differences in the structure and composition of cell-surface polysaccharides as compared with Sp245), a more rapid formation of GNPs was observed.
24276540	4	42	from	visible	833:839	arg1	course					755:760	the course	751:760	the course of cultivation with [AuCl4](-)	751:791	In the course of cultivation with [AuCl4](-), after 5 days, a mauve tint was already visible for cells of strain Sp245.5, after 6 days for Sp245 and after 10 days for Sp7.
24276540	5	43	theme	mutant	934:939	arg1	Sp245.5					948:954	the mutant strain Sp245.5	930:954	the mutant strain Sp245.5 (which has significant differences in the structure and composition of cell-surface polysaccharides as compared with Sp245)	930:1078	Thus, for the mutant strain Sp245.5 (which has significant differences in the structure and composition of cell-surface polysaccharides as compared with Sp245), a more rapid formation of GNPs was observed.
24276540	7	44	theme	[AuCl4	1567:1572	arg1	course					1557:1562	the course	1553:1562	the course of [AuCl4](-) reduction to Au(0)	1553:1595	Since by the time of GNP formation in our experiments the cells were found to be already not viable, this confirms the dominating role of cell surface structure and chemical composition in shaping the GNPs formed in the course of [AuCl4](-) reduction to Au(0).
24276540	7	45	dep	shaping	1526:1532	arg1	GNPs					1538:1541	the GNPs	1534:1541	shaping the GNPs formed in the course of [AuCl4](-) reduction to Au(0)	1526:1595	Since by the time of GNP formation in our experiments the cells were found to be already not viable, this confirms the dominating role of cell surface structure and chemical composition in shaping the GNPs formed in the course of [AuCl4](-) reduction to Au(0).
24276540	7	46	theme	reduction	1578:1586	arg1	[AuCl4					1567:1572	[AuCl4]	1567:1573	[AuCl4]	1567:1573	Since by the time of GNP formation in our experiments the cells were found to be already not viable, this confirms the dominating role of cell surface structure and chemical composition in shaping the GNPs formed in the course of [AuCl4](-) reduction to Au(0).
24276540	3	47	theme	cell	599:602	arg1	biomass					604:610	cell biomass	599:610	cell biomass	599:610	For the widely studied agriculturally important wild-type strains A. brasilense Sp7 and Sp245, GNPs formed after 10 days of incubation of cell biomass with 0.25 mM [AuCl4](-) were shown (using TEM) to be mainly of spherical form (5 to 20 nm in diameter), with rare occasional triangles.
24276540	5	48	theme	cell-surface	1027:1038	arg1	polysaccharides					1040:1054	cell-surface polysaccharides	1027:1054	cell-surface polysaccharides	1027:1054	Thus, for the mutant strain Sp245.5 (which has significant differences in the structure and composition of cell-surface polysaccharides as compared with Sp245), a more rapid formation of GNPs was observed.
24276540	9	49	theme	nanoparticles	1845:1857	arg1	synthesis					1832:1840	environmentally friendly synthesis	1807:1840	environmentally friendly synthesis of nanoparticles and possible use of bacterial cells with modified surface structure and composition for their fabrication	1807:1963	The data obtained may also help in developing protocols for environmentally friendly synthesis of nanoparticles and possible use of bacterial cells with modified surface structure and composition for their fabrication.
24276540	9	50	theme	possible	1863:1870	arg1	use					1872:1874	possible use	1863:1874	possible use of bacterial cells with modified surface structure and composition for their fabrication	1863:1963	The data obtained may also help in developing protocols for environmentally friendly synthesis of nanoparticles and possible use of bacterial cells with modified surface structure and composition for their fabrication.
24276540	7	51	theme	composition	1511:1521	arg1	role					1467:1470	the dominating role	1452:1470	the dominating role of cell surface structure and chemical composition in shaping the GNPs formed in the course of [AuCl4](-) reduction to Au(0)	1452:1595	Since by the time of GNP formation in our experiments the cells were found to be already not viable, this confirms the dominating role of cell surface structure and chemical composition in shaping the GNPs formed in the course of [AuCl4](-) reduction to Au(0).
24276540	7	52	from	role	1467:1470	arg1	shaping					1526:1532	shaping the GNPs	1526:1541	shaping the GNPs formed in the course of [AuCl4](-) reduction to Au(0)	1526:1595	Since by the time of GNP formation in our experiments the cells were found to be already not viable, this confirms the dominating role of cell surface structure and chemical composition in shaping the GNPs formed in the course of [AuCl4](-) reduction to Au(0).
24276540	3	53	theme	wild-type	509:517	arg1	Sp7					541:543	Sp7	541:543	Sp7	541:543	For the widely studied agriculturally important wild-type strains A. brasilense Sp7 and Sp245, GNPs formed after 10 days of incubation of cell biomass with 0.25 mM [AuCl4](-) were shown (using TEM) to be mainly of spherical form (5 to 20 nm in diameter), with rare occasional triangles.
24276540	3	53	theme	wild-type	509:517	arg1	Sp245					549:553	Sp245	549:553	Sp245	549:553	For the widely studied agriculturally important wild-type strains A. brasilense Sp7 and Sp245, GNPs formed after 10 days of incubation of cell biomass with 0.25 mM [AuCl4](-) were shown (using TEM) to be mainly of spherical form (5 to 20 nm in diameter), with rare occasional triangles.
24276540	3	53	theme	wild-type	509:517	arg1	strains					519:525	the widely studied agriculturally important wild-type strains	465:525	the widely studied agriculturally important wild-type strains A. brasilense Sp7 and Sp245	465:553	For the widely studied agriculturally important wild-type strains A. brasilense Sp7 and Sp245, GNPs formed after 10 days of incubation of cell biomass with 0.25 mM [AuCl4](-) were shown (using TEM) to be mainly of spherical form (5 to 20 nm in diameter), with rare occasional triangles.
24276540	6	54	theme	flower-like	1289:1299	arg1	nanoparticles					1301:1313	larger round-shaped flower-like nanoparticles	1269:1313	larger round-shaped flower-like nanoparticles about 100 nm in size	1269:1334	Moreover, their TEM images (also obtained after 10 days) showed different shapes: nano-sized spheres, triangles, hexagons and rods, as well as larger round-shaped flower-like nanoparticles about 100 nm in size.
24276540	9	55	theme	cells	1889:1893	arg1	nanoparticles					1845:1857	nanoparticles	1845:1857	nanoparticles	1845:1857	The data obtained may also help in developing protocols for environmentally friendly synthesis of nanoparticles and possible use of bacterial cells with modified surface structure and composition for their fabrication.
24276540	9	55	theme	cells	1889:1893	arg1	use					1872:1874	possible use	1863:1874	possible use of bacterial cells with modified surface structure and composition for their fabrication	1863:1963	The data obtained may also help in developing protocols for environmentally friendly synthesis of nanoparticles and possible use of bacterial cells with modified surface structure and composition for their fabrication.
24276540	6	56	theme	larger	1269:1274	arg1	nanoparticles					1301:1313	larger round-shaped flower-like nanoparticles	1269:1313	larger round-shaped flower-like nanoparticles about 100 nm in size	1269:1334	Moreover, their TEM images (also obtained after 10 days) showed different shapes: nano-sized spheres, triangles, hexagons and rods, as well as larger round-shaped flower-like nanoparticles about 100 nm in size.
24276540	9	57	theme	modified	1900:1907	arg1	structure					1917:1925	modified surface structure	1900:1925	modified surface structure	1900:1925	The data obtained may also help in developing protocols for environmentally friendly synthesis of nanoparticles and possible use of bacterial cells with modified surface structure and composition for their fabrication.
24276540	3	58	dep	form	685:688	arg1	20 nm					696:700	20 nm	696:700	20 nm	696:700	For the widely studied agriculturally important wild-type strains A. brasilense Sp7 and Sp245, GNPs formed after 10 days of incubation of cell biomass with 0.25 mM [AuCl4](-) were shown (using TEM) to be mainly of spherical form (5 to 20 nm in diameter), with rare occasional triangles.
24276540	3	59	with	incubation	585:594	arg1	[AuCl4					625:630	0.25 mM [AuCl4]	617:631	0.25 mM [AuCl4](-)	617:634	For the widely studied agriculturally important wild-type strains A. brasilense Sp7 and Sp245, GNPs formed after 10 days of incubation of cell biomass with 0.25 mM [AuCl4](-) were shown (using TEM) to be mainly of spherical form (5 to 20 nm in diameter), with rare occasional triangles.
24276540	7	60	theme	GNP	1358:1360	arg1	formation					1362:1370	GNP formation	1358:1370	GNP formation in our experiments	1358:1389	Since by the time of GNP formation in our experiments the cells were found to be already not viable, this confirms the dominating role of cell surface structure and chemical composition in shaping the GNPs formed in the course of [AuCl4](-) reduction to Au(0).
24276540	5	61	dep	structure	998:1006	arg1	the					994:996	the	994:996	the	994:996	Thus, for the mutant strain Sp245.5 (which has significant differences in the structure and composition of cell-surface polysaccharides as compared with Sp245), a more rapid formation of GNPs was observed.
24276540	3	62	dep	20 nm	696:700	arg1	to					693:694	to	693:694	to	693:694	For the widely studied agriculturally important wild-type strains A. brasilense Sp7 and Sp245, GNPs formed after 10 days of incubation of cell biomass with 0.25 mM [AuCl4](-) were shown (using TEM) to be mainly of spherical form (5 to 20 nm in diameter), with rare occasional triangles.
24276540	7	63	theme	structure	1488:1496	arg1	role					1467:1470	the dominating role	1452:1470	the dominating role of cell surface structure and chemical composition in shaping the GNPs formed in the course of [AuCl4](-) reduction to Au(0)	1452:1595	Since by the time of GNP formation in our experiments the cells were found to be already not viable, this confirms the dominating role of cell surface structure and chemical composition in shaping the GNPs formed in the course of [AuCl4](-) reduction to Au(0).
24276540	8	64	theme	biogeochemical	1655:1668	arg1	mechanisms					1670:1679	the natural biogeochemical mechanisms	1643:1679	the natural biogeochemical mechanisms of gold reduction and formation of GNPs involving microorganisms	1643:1744	This finding may be useful for understanding the natural biogeochemical mechanisms of gold reduction and formation of GNPs involving microorganisms.
24276540	3	65	with	form	685:688	arg1	triangles					737:745	rare occasional triangles	721:745	rare occasional triangles	721:745	For the widely studied agriculturally important wild-type strains A. brasilense Sp7 and Sp245, GNPs formed after 10 days of incubation of cell biomass with 0.25 mM [AuCl4](-) were shown (using TEM) to be mainly of spherical form (5 to 20 nm in diameter), with rare occasional triangles.
24276540	1	66	theme	nitrogen-fixing	121:135	arg1	bacterium					137:145	soil nitrogen-fixing bacterium	116:145	the soil nitrogen-fixing bacterium Azospirillum brasilense	112:169	For the soil nitrogen-fixing bacterium Azospirillum brasilense, the ability to reduce [AuCl4](-) and to form gold nanoparticles (GNPs) has been demonstrated, with the appearance of a mauve tint of the culture.
24276540	7	67	theme	cell	1475:1478	arg1	structure					1488:1496	cell surface structure	1475:1496	cell surface structure	1475:1496	Since by the time of GNP formation in our experiments the cells were found to be already not viable, this confirms the dominating role of cell surface structure and chemical composition in shaping the GNPs formed in the course of [AuCl4](-) reduction to Au(0).
24276540	2	68	theme	X-ray	422:426	arg1	analysis					441:448	X-ray fluorescence analysis	422:448	X-ray fluorescence analysis	422:448	To validate the shapes and chemical nature of nanoparticles, transmission electron microscopy (TEM) and X-ray fluorescence analysis were used.
24276540	8	69	theme	gold	1684:1687	arg1	reduction					1689:1697	gold reduction	1684:1697	gold reduction	1684:1697	This finding may be useful for understanding the natural biogeochemical mechanisms of gold reduction and formation of GNPs involving microorganisms.
24276540	1	70	theme	Azospirillum	147:158	arg1	brasilense					160:169	the soil nitrogen-fixing bacterium Azospirillum brasilense	112:169	the soil nitrogen-fixing bacterium Azospirillum brasilense	112:169	For the soil nitrogen-fixing bacterium Azospirillum brasilense, the ability to reduce [AuCl4](-) and to form gold nanoparticles (GNPs) has been demonstrated, with the appearance of a mauve tint of the culture.
24276540	8	71	theme	reduction	1689:1697	arg1	mechanisms					1670:1679	the natural biogeochemical mechanisms	1643:1679	the natural biogeochemical mechanisms of gold reduction and formation of GNPs involving microorganisms	1643:1744	This finding may be useful for understanding the natural biogeochemical mechanisms of gold reduction and formation of GNPs involving microorganisms.
24276540	1	72	theme	mauve	291:295	arg1	tint					297:300	a mauve tint	289:300	a mauve tint of the culture	289:315	For the soil nitrogen-fixing bacterium Azospirillum brasilense, the ability to reduce [AuCl4](-) and to form gold nanoparticles (GNPs) has been demonstrated, with the appearance of a mauve tint of the culture.
24276540	6	73	dep	images	1146:1151	arg1	obtained					1159:1166	obtained	1159:1166	obtained after 10 days	1159:1180	Moreover, their TEM images (also obtained after 10 days) showed different shapes: nano-sized spheres, triangles, hexagons and rods, as well as larger round-shaped flower-like nanoparticles about 100 nm in size.
24276540	6	74	from	nanoparticles	1301:1313	arg1	size					1331:1334	size	1331:1334	size	1331:1334	Moreover, their TEM images (also obtained after 10 days) showed different shapes: nano-sized spheres, triangles, hexagons and rods, as well as larger round-shaped flower-like nanoparticles about 100 nm in size.
24276540	0	75	theme	nanoparticles	93:105	arg1	formation					75:83	the formation	71:83	the formation of gold nanoparticles	71:105	Gold(III) reduction by the rhizobacterium Azospirillum brasilense with the formation of gold nanoparticles.
24276540	7	76	from	experiments	1379:1389	arg1	time					1350:1353	the time	1346:1353	the time of GNP formation in our experiments	1346:1389	Since by the time of GNP formation in our experiments the cells were found to be already not viable, this confirms the dominating role of cell surface structure and chemical composition in shaping the GNPs formed in the course of [AuCl4](-) reduction to Au(0).
24276540	4	77	dep	[AuCl4	782:787	arg1	-					790:790	-	790:790	-	790:790	In the course of cultivation with [AuCl4](-), after 5 days, a mauve tint was already visible for cells of strain Sp245.5, after 6 days for Sp245 and after 10 days for Sp7.
24276540	8	78	theme	GNPs	1716:1719	arg1	reduction					1689:1697	gold reduction	1684:1697	gold reduction	1684:1697	This finding may be useful for understanding the natural biogeochemical mechanisms of gold reduction and formation of GNPs involving microorganisms.
24276540	8	78	theme	GNPs	1716:1719	arg1	formation					1703:1711	formation	1703:1711	formation of GNPs involving microorganisms	1703:1744	This finding may be useful for understanding the natural biogeochemical mechanisms of gold reduction and formation of GNPs involving microorganisms.
24276540	1	79	theme	culture	309:315	arg1	tint					297:300	a mauve tint	289:300	a mauve tint of the culture	289:315	For the soil nitrogen-fixing bacterium Azospirillum brasilense, the ability to reduce [AuCl4](-) and to form gold nanoparticles (GNPs) has been demonstrated, with the appearance of a mauve tint of the culture.
24276540	9	80	theme	environmentally	1807:1821	arg1	synthesis					1832:1840	environmentally friendly synthesis	1807:1840	environmentally friendly synthesis of nanoparticles and possible use of bacterial cells with modified surface structure and composition for their fabrication	1807:1963	The data obtained may also help in developing protocols for environmentally friendly synthesis of nanoparticles and possible use of bacterial cells with modified surface structure and composition for their fabrication.
24276540	5	81	theme	rapid	1088:1092	arg1	formation					1094:1102	a more rapid formation	1081:1102	a more rapid formation of GNPs	1081:1110	Thus, for the mutant strain Sp245.5 (which has significant differences in the structure and composition of cell-surface polysaccharides as compared with Sp245), a more rapid formation of GNPs was observed.
24276540	6	82	from	shapes	1200:1205	arg1	size					1331:1334	size	1331:1334	size	1331:1334	Moreover, their TEM images (also obtained after 10 days) showed different shapes: nano-sized spheres, triangles, hexagons and rods, as well as larger round-shaped flower-like nanoparticles about 100 nm in size.
24276540	3	83	dep	[AuCl4	625:630	arg1	-					633:633	-	633:633	-	633:633	For the widely studied agriculturally important wild-type strains A. brasilense Sp7 and Sp245, GNPs formed after 10 days of incubation of cell biomass with 0.25 mM [AuCl4](-) were shown (using TEM) to be mainly of spherical form (5 to 20 nm in diameter), with rare occasional triangles.
24276540	3	84	from	20 nm	696:700	arg1	diameter					705:712	diameter	705:712	diameter	705:712	For the widely studied agriculturally important wild-type strains A. brasilense Sp7 and Sp245, GNPs formed after 10 days of incubation of cell biomass with 0.25 mM [AuCl4](-) were shown (using TEM) to be mainly of spherical form (5 to 20 nm in diameter), with rare occasional triangles.
24276540	2	85	theme	chemical	345:352	arg1	nature					354:359	chemical nature	345:359	chemical nature	345:359	To validate the shapes and chemical nature of nanoparticles, transmission electron microscopy (TEM) and X-ray fluorescence analysis were used.
24276540	6	86	theme	different	1190:1198	arg1	shapes					1200:1205	different shapes	1190:1205	different shapes	1190:1205	Moreover, their TEM images (also obtained after 10 days) showed different shapes: nano-sized spheres, triangles, hexagons and rods, as well as larger round-shaped flower-like nanoparticles about 100 nm in size.
24276540	6	87	from	rods	1252:1255	arg1	size					1331:1334	size	1331:1334	size	1331:1334	Moreover, their TEM images (also obtained after 10 days) showed different shapes: nano-sized spheres, triangles, hexagons and rods, as well as larger round-shaped flower-like nanoparticles about 100 nm in size.
24276540	2	88	used	used	455:458	arg2	TEM					413:415	TEM	413:415	TEM	413:415	To validate the shapes and chemical nature of nanoparticles, transmission electron microscopy (TEM) and X-ray fluorescence analysis were used.
24276540	2	88	used	used	455:458	arg2	microscopy					401:410	transmission electron microscopy	379:410	transmission electron microscopy (TEM)	379:416	To validate the shapes and chemical nature of nanoparticles, transmission electron microscopy (TEM) and X-ray fluorescence analysis were used.
24276540	2	88	used	used	455:458	arg2	analysis					441:448	X-ray fluorescence analysis	422:448	X-ray fluorescence analysis	422:448	To validate the shapes and chemical nature of nanoparticles, transmission electron microscopy (TEM) and X-ray fluorescence analysis were used.
24276540	2	89	dep	shapes	334:339	arg1	the					330:332	the	330:332	the	330:332	To validate the shapes and chemical nature of nanoparticles, transmission electron microscopy (TEM) and X-ray fluorescence analysis were used.
24276540	0	90	theme	rhizobacterium	27:40	arg1	Azospirillum					42:53	the rhizobacterium Azospirillum	23:53	the rhizobacterium Azospirillum	23:53	Gold(III) reduction by the rhizobacterium Azospirillum brasilense with the formation of gold nanoparticles.
24276540	5	91	theme	strain	941:946	arg1	Sp245.5					948:954	the mutant strain Sp245.5	930:954	the mutant strain Sp245.5 (which has significant differences in the structure and composition of cell-surface polysaccharides as compared with Sp245)	930:1078	Thus, for the mutant strain Sp245.5 (which has significant differences in the structure and composition of cell-surface polysaccharides as compared with Sp245), a more rapid formation of GNPs was observed.
24276540	2	92	theme	electron	392:399	arg1	microscopy					401:410	transmission electron microscopy	379:410	transmission electron microscopy (TEM)	379:416	To validate the shapes and chemical nature of nanoparticles, transmission electron microscopy (TEM) and X-ray fluorescence analysis were used.
24276540	2	92	theme	electron	392:399	arg1	TEM					413:415	TEM	413:415	TEM	413:415	To validate the shapes and chemical nature of nanoparticles, transmission electron microscopy (TEM) and X-ray fluorescence analysis were used.
24276540	3	93	theme	biomass	604:610	arg1	incubation					585:594	incubation	585:594	incubation of cell biomass with 0.25 mM [AuCl4](-)	585:634	For the widely studied agriculturally important wild-type strains A. brasilense Sp7 and Sp245, GNPs formed after 10 days of incubation of cell biomass with 0.25 mM [AuCl4](-) were shown (using TEM) to be mainly of spherical form (5 to 20 nm in diameter), with rare occasional triangles.
24276540	3	94	dep	shown	641:645	arg1	TEM					654:656	TEM	654:656	TEM	654:656	For the widely studied agriculturally important wild-type strains A. brasilense Sp7 and Sp245, GNPs formed after 10 days of incubation of cell biomass with 0.25 mM [AuCl4](-) were shown (using TEM) to be mainly of spherical form (5 to 20 nm in diameter), with rare occasional triangles.
24276540	7	95	from	time	1350:1353	arg1	experiments					1379:1389	our experiments	1375:1389	our experiments	1375:1389	Since by the time of GNP formation in our experiments the cells were found to be already not viable, this confirms the dominating role of cell surface structure and chemical composition in shaping the GNPs formed in the course of [AuCl4](-) reduction to Au(0).
24276540	6	96	from	triangles	1228:1236	arg1	size					1331:1334	size	1331:1334	size	1331:1334	Moreover, their TEM images (also obtained after 10 days) showed different shapes: nano-sized spheres, triangles, hexagons and rods, as well as larger round-shaped flower-like nanoparticles about 100 nm in size.
24276540	6	97	from	hexagons	1239:1246	arg1	size					1331:1334	size	1331:1334	size	1331:1334	Moreover, their TEM images (also obtained after 10 days) showed different shapes: nano-sized spheres, triangles, hexagons and rods, as well as larger round-shaped flower-like nanoparticles about 100 nm in size.
24276540	9	98	theme	friendly	1823:1830	arg1	synthesis					1832:1840	environmentally friendly synthesis	1807:1840	environmentally friendly synthesis of nanoparticles and possible use of bacterial cells with modified surface structure and composition for their fabrication	1807:1963	The data obtained may also help in developing protocols for environmentally friendly synthesis of nanoparticles and possible use of bacterial cells with modified surface structure and composition for their fabrication.
24276540	1	99	dep	[AuCl4	194:199	arg1	-					202:202	-	202:202	-	202:202	For the soil nitrogen-fixing bacterium Azospirillum brasilense, the ability to reduce [AuCl4](-) and to form gold nanoparticles (GNPs) has been demonstrated, with the appearance of a mauve tint of the culture.
24276540	5	100	theme	polysaccharides	1040:1054	arg1	structure					998:1006	structure	998:1006	structure	998:1006	Thus, for the mutant strain Sp245.5 (which has significant differences in the structure and composition of cell-surface polysaccharides as compared with Sp245), a more rapid formation of GNPs was observed.
24276540	5	100	theme	polysaccharides	1040:1054	arg1	composition					1012:1022	composition	1012:1022	composition	1012:1022	Thus, for the mutant strain Sp245.5 (which has significant differences in the structure and composition of cell-surface polysaccharides as compared with Sp245), a more rapid formation of GNPs was observed.
25265339	4	0	theme	different	797:805	arg1	doses					807:811	different doses	797:811	different doses of JRP1	797:819	The results in vivo showed that compared with those in the control group, the inhibition rates of different doses of JRP1 on S180 cells in the tumor-bearing mice were 35.3%, 40.6% and 48.1%, respectively, and serum immune cytokine levels such as IL-2, TNF-α and IFN-γ were significantly improved.
25265339	4	1	from	cells	829:833	arg1	mice					856:859	the tumor-bearing mice	838:859	the tumor-bearing mice	838:859	The results in vivo showed that compared with those in the control group, the inhibition rates of different doses of JRP1 on S180 cells in the tumor-bearing mice were 35.3%, 40.6% and 48.1%, respectively, and serum immune cytokine levels such as IL-2, TNF-α and IFN-γ were significantly improved.
25265339	4	2	theme	tumor-bearing	842:854	arg1	mice					856:859	the tumor-bearing mice	838:859	the tumor-bearing mice	838:859	The results in vivo showed that compared with those in the control group, the inhibition rates of different doses of JRP1 on S180 cells in the tumor-bearing mice were 35.3%, 40.6% and 48.1%, respectively, and serum immune cytokine levels such as IL-2, TNF-α and IFN-γ were significantly improved.
25265339	2	3	with	JRP1	305:308	arg1	GC					335:336	GC	335:336	GC	335:336	The determination of the monosaccharide composition in JRP1 with gas chromatography (GC) showed that JRP1 was composed of Gal (43.1%), Glu (23.6%), Ara (16.2%), Rha (9.8%) and Fru (7.3%).
25265339	2	3	with	JRP1	305:308	arg1	chromatography					319:332	gas chromatography	315:332	gas chromatography (GC)	315:337	The determination of the monosaccharide composition in JRP1 with gas chromatography (GC) showed that JRP1 was composed of Gal (43.1%), Glu (23.6%), Ara (16.2%), Rha (9.8%) and Fru (7.3%).
25265339	4	4	from	results	703:709	arg1	vivo					714:717	vivo	714:717	vivo	714:717	The results in vivo showed that compared with those in the control group, the inhibition rates of different doses of JRP1 on S180 cells in the tumor-bearing mice were 35.3%, 40.6% and 48.1%, respectively, and serum immune cytokine levels such as IL-2, TNF-α and IFN-γ were significantly improved.
25265339	3	5	theme	significant	516:526	arg1	inhibition					528:537	a significant inhibition	514:537	a significant inhibition	514:537	The results in vitro showed that 25, 50 and 100 μg/mL of JRP1 could present a significant inhibition on the growth of S180 cells, and furthermore, a significant improvement on the proliferation ability of lymphocytes and the phagocytic activity of macrophages.
25265339	5	6	dep	effective	1048:1056	arg1	antitumor					1058:1066	antitumor	1058:1066	antitumor	1058:1066	Our results confirm that JRP1 has the activities of effective antitumor and immunomodulatory function.
25265339	2	7	theme	composition	290:300	arg1	determination					254:266	The determination	250:266	The determination of the monosaccharide composition in JRP1 with gas chromatography (GC)	250:337	The determination of the monosaccharide composition in JRP1 with gas chromatography (GC) showed that JRP1 was composed of Gal (43.1%), Glu (23.6%), Ara (16.2%), Rha (9.8%) and Fru (7.3%).
25265339	4	8	theme	control	758:764	arg1	group					766:770	the control group	754:770	the control group	754:770	The results in vivo showed that compared with those in the control group, the inhibition rates of different doses of JRP1 on S180 cells in the tumor-bearing mice were 35.3%, 40.6% and 48.1%, respectively, and serum immune cytokine levels such as IL-2, TNF-α and IFN-γ were significantly improved.
25265339	3	9	from	improvement	599:609	arg1	ability					632:638	the proliferation ability	614:638	the proliferation ability of lymphocytes	614:653	The results in vitro showed that 25, 50 and 100 μg/mL of JRP1 could present a significant inhibition on the growth of S180 cells, and furthermore, a significant improvement on the proliferation ability of lymphocytes and the phagocytic activity of macrophages.
25265339	3	9	from	improvement	599:609	arg1	activity					674:681	the phagocytic activity	659:681	the phagocytic activity of macrophages	659:696	The results in vitro showed that 25, 50 and 100 μg/mL of JRP1 could present a significant inhibition on the growth of S180 cells, and furthermore, a significant improvement on the proliferation ability of lymphocytes and the phagocytic activity of macrophages.
25265339	2	10	theme	monosaccharide	275:288	arg1	composition					290:300	the monosaccharide composition	271:300	the monosaccharide composition in JRP1 with gas chromatography (GC)	271:337	The determination of the monosaccharide composition in JRP1 with gas chromatography (GC) showed that JRP1 was composed of Gal (43.1%), Glu (23.6%), Ara (16.2%), Rha (9.8%) and Fru (7.3%).
25265339	3	11	theme	lymphocytes	643:653	arg1	ability					632:638	the proliferation ability	614:638	the proliferation ability of lymphocytes	614:653	The results in vitro showed that 25, 50 and 100 μg/mL of JRP1 could present a significant inhibition on the growth of S180 cells, and furthermore, a significant improvement on the proliferation ability of lymphocytes and the phagocytic activity of macrophages.
25265339	3	11	theme	lymphocytes	643:653	arg1	activity					674:681	the phagocytic activity	659:681	the phagocytic activity of macrophages	659:696	The results in vitro showed that 25, 50 and 100 μg/mL of JRP1 could present a significant inhibition on the growth of S180 cells, and furthermore, a significant improvement on the proliferation ability of lymphocytes and the phagocytic activity of macrophages.
25265339	3	12	theme	S180	556:559	arg1	cells					561:565	S180 cells	556:565	S180 cells	556:565	The results in vitro showed that 25, 50 and 100 μg/mL of JRP1 could present a significant inhibition on the growth of S180 cells, and furthermore, a significant improvement on the proliferation ability of lymphocytes and the phagocytic activity of macrophages.
25265339	3	13	theme	phagocytic	663:672	arg1	activity					674:681	the phagocytic activity	659:681	the phagocytic activity of macrophages	659:696	The results in vitro showed that 25, 50 and 100 μg/mL of JRP1 could present a significant inhibition on the growth of S180 cells, and furthermore, a significant improvement on the proliferation ability of lymphocytes and the phagocytic activity of macrophages.
25265339	1	14	theme	Maxim	243:247	arg1	fruit					214:218	immature fruit	205:218	immature fruit of Juglans mandshurica Maxim	205:247	A water-soluble polysaccharide, named as JRP1, was extracted and fractioned from the epicarp of immature fruit of Juglans mandshurica Maxim.
25265339	4	15	theme	S180	824:827	arg1	cells					829:833	S180 cells	824:833	S180 cells in the tumor-bearing mice	824:859	The results in vivo showed that compared with those in the control group, the inhibition rates of different doses of JRP1 on S180 cells in the tumor-bearing mice were 35.3%, 40.6% and 48.1%, respectively, and serum immune cytokine levels such as IL-2, TNF-α and IFN-γ were significantly improved.
25265339	4	16	theme	serum	908:912	arg1	levels					930:935	serum immune cytokine levels	908:935	serum immune cytokine levels such as IL-2, TNF-α and IFN-γ	908:965	The results in vivo showed that compared with those in the control group, the inhibition rates of different doses of JRP1 on S180 cells in the tumor-bearing mice were 35.3%, 40.6% and 48.1%, respectively, and serum immune cytokine levels such as IL-2, TNF-α and IFN-γ were significantly improved.
25265339	4	16	theme	serum	908:912	arg1	IL-2					945:948	IL-2	945:948	IL-2	945:948	The results in vivo showed that compared with those in the control group, the inhibition rates of different doses of JRP1 on S180 cells in the tumor-bearing mice were 35.3%, 40.6% and 48.1%, respectively, and serum immune cytokine levels such as IL-2, TNF-α and IFN-γ were significantly improved.
25265339	4	16	theme	serum	908:912	arg1	IFN-γ					961:965	IFN-γ	961:965	IFN-γ	961:965	The results in vivo showed that compared with those in the control group, the inhibition rates of different doses of JRP1 on S180 cells in the tumor-bearing mice were 35.3%, 40.6% and 48.1%, respectively, and serum immune cytokine levels such as IL-2, TNF-α and IFN-γ were significantly improved.
25265339	4	16	theme	serum	908:912	arg1	TNF-α					951:955	TNF-α	951:955	TNF-α	951:955	The results in vivo showed that compared with those in the control group, the inhibition rates of different doses of JRP1 on S180 cells in the tumor-bearing mice were 35.3%, 40.6% and 48.1%, respectively, and serum immune cytokine levels such as IL-2, TNF-α and IFN-γ were significantly improved.
25265339	3	17	attach	present	506:512	arg1	growth					546:551	the growth	542:551	the growth of S180 cells	542:565	The results in vitro showed that 25, 50 and 100 μg/mL of JRP1 could present a significant inhibition on the growth of S180 cells, and furthermore, a significant improvement on the proliferation ability of lymphocytes and the phagocytic activity of macrophages.
25265339	3	17	attach	present	506:512	arg2	μg/mL					486:490	25, 50 and 100 μg/mL	471:490	25, 50 and 100 μg/mL of JRP1	471:498	The results in vitro showed that 25, 50 and 100 μg/mL of JRP1 could present a significant inhibition on the growth of S180 cells, and furthermore, a significant improvement on the proliferation ability of lymphocytes and the phagocytic activity of macrophages.
25265339	2	18	theme	gas	315:317	arg1	GC					335:336	GC	335:336	GC	335:336	The determination of the monosaccharide composition in JRP1 with gas chromatography (GC) showed that JRP1 was composed of Gal (43.1%), Glu (23.6%), Ara (16.2%), Rha (9.8%) and Fru (7.3%).
25265339	2	18	theme	gas	315:317	arg1	chromatography					319:332	gas chromatography	315:332	gas chromatography (GC)	315:337	The determination of the monosaccharide composition in JRP1 with gas chromatography (GC) showed that JRP1 was composed of Gal (43.1%), Glu (23.6%), Ara (16.2%), Rha (9.8%) and Fru (7.3%).
25265339	0	19	theme	Antitumor	0:8	arg1	effects					10:16	Antitumor effects	0:16	Antitumor effects	0:16	Antitumor effects and immune regulation activities of a purified polysaccharide extracted from Juglan regia.
25265339	0	20	theme	immune	22:27	arg1	activities					40:49	immune regulation activities	22:49	immune regulation activities	22:49	Antitumor effects and immune regulation activities of a purified polysaccharide extracted from Juglan regia.
25265339	3	21	theme	cells	561:565	arg1	growth					546:551	the growth	542:551	the growth of S180 cells	542:565	The results in vitro showed that 25, 50 and 100 μg/mL of JRP1 could present a significant inhibition on the growth of S180 cells, and furthermore, a significant improvement on the proliferation ability of lymphocytes and the phagocytic activity of macrophages.
25265339	2	22	from	determination	254:266	arg1	JRP1					305:308	JRP1	305:308	JRP1 with gas chromatography (GC)	305:337	The determination of the monosaccharide composition in JRP1 with gas chromatography (GC) showed that JRP1 was composed of Gal (43.1%), Glu (23.6%), Ara (16.2%), Rha (9.8%) and Fru (7.3%).
25265339	5	23	theme	function	1089:1096	arg1	activities					1034:1043	the activities	1030:1043	the activities of effective antitumor and immunomodulatory function	1030:1096	Our results confirm that JRP1 has the activities of effective antitumor and immunomodulatory function.
25265339	0	24	theme	regulation	29:38	arg1	activities					40:49	immune regulation activities	22:49	immune regulation activities	22:49	Antitumor effects and immune regulation activities of a purified polysaccharide extracted from Juglan regia.
25265339	3	25	theme	significant	587:597	arg1	improvement					599:609	a significant improvement	585:609	a significant improvement on the proliferation ability of lymphocytes and the phagocytic activity of macrophages	585:696	The results in vitro showed that 25, 50 and 100 μg/mL of JRP1 could present a significant inhibition on the growth of S180 cells, and furthermore, a significant improvement on the proliferation ability of lymphocytes and the phagocytic activity of macrophages.
25265339	4	26	theme	doses	807:811	arg1	rates					788:792	the inhibition rates	773:792	the inhibition rates of different doses of JRP1 on S180 cells in the tumor-bearing mice	773:859	The results in vivo showed that compared with those in the control group, the inhibition rates of different doses of JRP1 on S180 cells in the tumor-bearing mice were 35.3%, 40.6% and 48.1%, respectively, and serum immune cytokine levels such as IL-2, TNF-α and IFN-γ were significantly improved.
25265339	4	26	theme	doses	807:811	arg1	%					870:870	35.3%	866:870	35.3%	866:870	The results in vivo showed that compared with those in the control group, the inhibition rates of different doses of JRP1 on S180 cells in the tumor-bearing mice were 35.3%, 40.6% and 48.1%, respectively, and serum immune cytokine levels such as IL-2, TNF-α and IFN-γ were significantly improved.
25265339	1	27	theme	immature	205:212	arg1	fruit					214:218	immature fruit	205:218	immature fruit of Juglans mandshurica Maxim	205:247	A water-soluble polysaccharide, named as JRP1, was extracted and fractioned from the epicarp of immature fruit of Juglans mandshurica Maxim.
25265339	4	28	theme	inhibition	777:786	arg1	rates					788:792	the inhibition rates	773:792	the inhibition rates of different doses of JRP1 on S180 cells in the tumor-bearing mice	773:859	The results in vivo showed that compared with those in the control group, the inhibition rates of different doses of JRP1 on S180 cells in the tumor-bearing mice were 35.3%, 40.6% and 48.1%, respectively, and serum immune cytokine levels such as IL-2, TNF-α and IFN-γ were significantly improved.
25265339	4	28	theme	inhibition	777:786	arg1	%					870:870	35.3%	866:870	35.3%	866:870	The results in vivo showed that compared with those in the control group, the inhibition rates of different doses of JRP1 on S180 cells in the tumor-bearing mice were 35.3%, 40.6% and 48.1%, respectively, and serum immune cytokine levels such as IL-2, TNF-α and IFN-γ were significantly improved.
25265339	1	29	theme	water-soluble	111:123	arg1	polysaccharide					125:138	A water-soluble polysaccharide	109:138	A water-soluble polysaccharide	109:138	A water-soluble polysaccharide, named as JRP1, was extracted and fractioned from the epicarp of immature fruit of Juglans mandshurica Maxim.
25265339	4	30	theme	JRP1	816:819	arg1	doses					807:811	different doses	797:811	different doses of JRP1	797:819	The results in vivo showed that compared with those in the control group, the inhibition rates of different doses of JRP1 on S180 cells in the tumor-bearing mice were 35.3%, 40.6% and 48.1%, respectively, and serum immune cytokine levels such as IL-2, TNF-α and IFN-γ were significantly improved.
25265339	5	31	contain	has	1026:1028	arg1	JRP1					1021:1024	JRP1	1021:1024	JRP1	1021:1024	Our results confirm that JRP1 has the activities of effective antitumor and immunomodulatory function.
25265339	5	31	contain	has	1026:1028	arg2	activities					1034:1043	the activities	1030:1043	the activities of effective antitumor and immunomodulatory function	1030:1096	Our results confirm that JRP1 has the activities of effective antitumor and immunomodulatory function.
25265339	3	32	theme	macrophages	686:696	arg1	ability					632:638	the proliferation ability	614:638	the proliferation ability of lymphocytes	614:653	The results in vitro showed that 25, 50 and 100 μg/mL of JRP1 could present a significant inhibition on the growth of S180 cells, and furthermore, a significant improvement on the proliferation ability of lymphocytes and the phagocytic activity of macrophages.
25265339	3	32	theme	macrophages	686:696	arg1	activity					674:681	the phagocytic activity	659:681	the phagocytic activity of macrophages	659:696	The results in vitro showed that 25, 50 and 100 μg/mL of JRP1 could present a significant inhibition on the growth of S180 cells, and furthermore, a significant improvement on the proliferation ability of lymphocytes and the phagocytic activity of macrophages.
25265339	1	33	theme	fruit	214:218	arg1	epicarp					194:200	the epicarp	190:200	the epicarp of immature fruit of Juglans mandshurica Maxim	190:247	A water-soluble polysaccharide, named as JRP1, was extracted and fractioned from the epicarp of immature fruit of Juglans mandshurica Maxim.
25265339	4	34	theme	cytokine	921:928	arg1	levels					930:935	serum immune cytokine levels	908:935	serum immune cytokine levels such as IL-2, TNF-α and IFN-γ	908:965	The results in vivo showed that compared with those in the control group, the inhibition rates of different doses of JRP1 on S180 cells in the tumor-bearing mice were 35.3%, 40.6% and 48.1%, respectively, and serum immune cytokine levels such as IL-2, TNF-α and IFN-γ were significantly improved.
25265339	4	34	theme	cytokine	921:928	arg1	IL-2					945:948	IL-2	945:948	IL-2	945:948	The results in vivo showed that compared with those in the control group, the inhibition rates of different doses of JRP1 on S180 cells in the tumor-bearing mice were 35.3%, 40.6% and 48.1%, respectively, and serum immune cytokine levels such as IL-2, TNF-α and IFN-γ were significantly improved.
25265339	4	34	theme	cytokine	921:928	arg1	IFN-γ					961:965	IFN-γ	961:965	IFN-γ	961:965	The results in vivo showed that compared with those in the control group, the inhibition rates of different doses of JRP1 on S180 cells in the tumor-bearing mice were 35.3%, 40.6% and 48.1%, respectively, and serum immune cytokine levels such as IL-2, TNF-α and IFN-γ were significantly improved.
25265339	4	34	theme	cytokine	921:928	arg1	TNF-α					951:955	TNF-α	951:955	TNF-α	951:955	The results in vivo showed that compared with those in the control group, the inhibition rates of different doses of JRP1 on S180 cells in the tumor-bearing mice were 35.3%, 40.6% and 48.1%, respectively, and serum immune cytokine levels such as IL-2, TNF-α and IFN-γ were significantly improved.
25265339	2	35	from	composition	290:300	arg1	JRP1					305:308	JRP1	305:308	JRP1 with gas chromatography (GC)	305:337	The determination of the monosaccharide composition in JRP1 with gas chromatography (GC) showed that JRP1 was composed of Gal (43.1%), Glu (23.6%), Ara (16.2%), Rha (9.8%) and Fru (7.3%).
25265339	0	36	theme	polysaccharide	65:78	arg1	activities					40:49	immune regulation activities	22:49	immune regulation activities	22:49	Antitumor effects and immune regulation activities of a purified polysaccharide extracted from Juglan regia.
25265339	0	36	theme	polysaccharide	65:78	arg1	effects					10:16	Antitumor effects	0:16	Antitumor effects	0:16	Antitumor effects and immune regulation activities of a purified polysaccharide extracted from Juglan regia.
25265339	3	37	theme	JRP1	495:498	arg1	μg/mL					486:490	25, 50 and 100 μg/mL	471:490	25, 50 and 100 μg/mL of JRP1	471:498	The results in vitro showed that 25, 50 and 100 μg/mL of JRP1 could present a significant inhibition on the growth of S180 cells, and furthermore, a significant improvement on the proliferation ability of lymphocytes and the phagocytic activity of macrophages.
25265339	4	38	theme	immune	914:919	arg1	levels					930:935	serum immune cytokine levels	908:935	serum immune cytokine levels such as IL-2, TNF-α and IFN-γ	908:965	The results in vivo showed that compared with those in the control group, the inhibition rates of different doses of JRP1 on S180 cells in the tumor-bearing mice were 35.3%, 40.6% and 48.1%, respectively, and serum immune cytokine levels such as IL-2, TNF-α and IFN-γ were significantly improved.
25265339	4	38	theme	immune	914:919	arg1	IL-2					945:948	IL-2	945:948	IL-2	945:948	The results in vivo showed that compared with those in the control group, the inhibition rates of different doses of JRP1 on S180 cells in the tumor-bearing mice were 35.3%, 40.6% and 48.1%, respectively, and serum immune cytokine levels such as IL-2, TNF-α and IFN-γ were significantly improved.
25265339	4	38	theme	immune	914:919	arg1	IFN-γ					961:965	IFN-γ	961:965	IFN-γ	961:965	The results in vivo showed that compared with those in the control group, the inhibition rates of different doses of JRP1 on S180 cells in the tumor-bearing mice were 35.3%, 40.6% and 48.1%, respectively, and serum immune cytokine levels such as IL-2, TNF-α and IFN-γ were significantly improved.
25265339	4	38	theme	immune	914:919	arg1	TNF-α					951:955	TNF-α	951:955	TNF-α	951:955	The results in vivo showed that compared with those in the control group, the inhibition rates of different doses of JRP1 on S180 cells in the tumor-bearing mice were 35.3%, 40.6% and 48.1%, respectively, and serum immune cytokine levels such as IL-2, TNF-α and IFN-γ were significantly improved.
25265339	5	39	theme	effective	1048:1056	arg1	function					1089:1096	effective antitumor and immunomodulatory function	1048:1096	effective antitumor and immunomodulatory function	1048:1096	Our results confirm that JRP1 has the activities of effective antitumor and immunomodulatory function.
25265339	4	40	from	rates	788:792	arg1	cells					829:833	S180 cells	824:833	S180 cells in the tumor-bearing mice	824:859	The results in vivo showed that compared with those in the control group, the inhibition rates of different doses of JRP1 on S180 cells in the tumor-bearing mice were 35.3%, 40.6% and 48.1%, respectively, and serum immune cytokine levels such as IL-2, TNF-α and IFN-γ were significantly improved.
25265339	0	41	theme	purified	56:63	arg1	polysaccharide					65:78	a purified polysaccharide	54:78	a purified polysaccharide	54:78	Antitumor effects and immune regulation activities of a purified polysaccharide extracted from Juglan regia.
25265339	5	42	theme	immunomodulatory	1072:1087	arg1	function					1089:1096	effective antitumor and immunomodulatory function	1048:1096	effective antitumor and immunomodulatory function	1048:1096	Our results confirm that JRP1 has the activities of effective antitumor and immunomodulatory function.
25265339	3	43	theme	proliferation	618:630	arg1	ability					632:638	the proliferation ability	614:638	the proliferation ability of lymphocytes	614:653	The results in vitro showed that 25, 50 and 100 μg/mL of JRP1 could present a significant inhibition on the growth of S180 cells, and furthermore, a significant improvement on the proliferation ability of lymphocytes and the phagocytic activity of macrophages.
25265339	2	44	from	JRP1	305:308	arg1	determination					254:266	The determination	250:266	The determination of the monosaccharide composition in JRP1 with gas chromatography (GC)	250:337	The determination of the monosaccharide composition in JRP1 with gas chromatography (GC) showed that JRP1 was composed of Gal (43.1%), Glu (23.6%), Ara (16.2%), Rha (9.8%) and Fru (7.3%).
24331712	2	0	theme	glycol	660:665	arg1	MGC					677:679	MGC	677:679	MGC	677:679	With this focus, the primary aim of the current study was to characterize the response of human ASCs encapsulated within three-dimensional bioscaffolds incorporating decellularized adipose tissue (DAT) as a bioactive matrix within photo-cross-linkable methacrylated glycol chitosan (MGC) or methacrylated chondroitin sulphate (MCS) delivery vehicles.
24331712	2	0	theme	glycol	660:665	arg1	chitosan					667:674	photo-cross-linkable methacrylated glycol chitosan	625:674	photo-cross-linkable methacrylated glycol chitosan (MGC)	625:680	With this focus, the primary aim of the current study was to characterize the response of human ASCs encapsulated within three-dimensional bioscaffolds incorporating decellularized adipose tissue (DAT) as a bioactive matrix within photo-cross-linkable methacrylated glycol chitosan (MGC) or methacrylated chondroitin sulphate (MCS) delivery vehicles.
24331712	0	1	link	adipose-derived	106:120	arg1	cells					127:131	adipose-derived stem cells	106:131	adipose-derived stem cells	106:131	Composite hydrogel scaffolds incorporating decellularized adipose tissue for soft tissue engineering with adipose-derived stem cells.
24331712	4	2	theme	minimally-invasive	1100:1117	arg1	uniformity					1060:1069	uniformity	1060:1069	uniformity	1060:1069	The encapsulation strategy allows for tuning of the 3-D microenvironment and provides an effective method of cell delivery with high seeding efficiency and uniformity, which could be adapted as a minimally-invasive in situ approach.
24331712	4	2	theme	minimally-invasive	1100:1117	arg1	approach					1127:1134	a minimally-invasive in situ approach	1098:1134	a minimally-invasive in situ approach	1098:1134	The encapsulation strategy allows for tuning of the 3-D microenvironment and provides an effective method of cell delivery with high seeding efficiency and uniformity, which could be adapted as a minimally-invasive in situ approach.
24331712	4	2	theme	minimally-invasive	1100:1117	arg1	efficiency					1045:1054	high seeding efficiency	1032:1054	high seeding efficiency	1032:1054	The encapsulation strategy allows for tuning of the 3-D microenvironment and provides an effective method of cell delivery with high seeding efficiency and uniformity, which could be adapted as a minimally-invasive in situ approach.
24331712	8	3	theme	In	1685:1686	arg1	analysis					1693:1700	In vivo analysis	1685:1700	In vivo analysis in a subcutaneous Wistar rat model at 1, 4 and 12 weeks	1685:1756	In vivo analysis in a subcutaneous Wistar rat model at 1, 4 and 12 weeks showed superior implant integration and adipogenesis in the MCS-based composites, with allogenic ASCs promoting cell infiltration, angiogenesis and ultimately, fat formation.
24331712	5	4	theme	dehydrogenase	1258:1270	arg1	days					1209:1212	14 days	1206:1212	14 days in terms of viability	1206:1234	Through in vitro cell culture studies, human ASCs were assessed over 14 days in terms of viability, glycerol-3-phosphate dehydrogenase (GPDH) enzyme activity, adipogenic gene expression and intracellular lipid accumulation.
24331712	5	4	theme	dehydrogenase	1258:1270	arg1	activity					1286:1293	glycerol-3-phosphate dehydrogenase (GPDH) enzyme activity	1237:1293	glycerol-3-phosphate dehydrogenase (GPDH) enzyme activity	1237:1293	Through in vitro cell culture studies, human ASCs were assessed over 14 days in terms of viability, glycerol-3-phosphate dehydrogenase (GPDH) enzyme activity, adipogenic gene expression and intracellular lipid accumulation.
24331712	2	5	theme	photo-cross-linkable	625:644	arg1	MGC					677:679	MGC	677:679	MGC	677:679	With this focus, the primary aim of the current study was to characterize the response of human ASCs encapsulated within three-dimensional bioscaffolds incorporating decellularized adipose tissue (DAT) as a bioactive matrix within photo-cross-linkable methacrylated glycol chitosan (MGC) or methacrylated chondroitin sulphate (MCS) delivery vehicles.
24331712	2	5	theme	photo-cross-linkable	625:644	arg1	chitosan					667:674	photo-cross-linkable methacrylated glycol chitosan	625:674	photo-cross-linkable methacrylated glycol chitosan (MGC)	625:680	With this focus, the primary aim of the current study was to characterize the response of human ASCs encapsulated within three-dimensional bioscaffolds incorporating decellularized adipose tissue (DAT) as a bioactive matrix within photo-cross-linkable methacrylated glycol chitosan (MGC) or methacrylated chondroitin sulphate (MCS) delivery vehicles.
24331712	3	6	theme	composite	771:779	arg1	scaffolds					781:789	Stable MGC- and MCS-based composite scaffolds	745:789	Stable MGC- and MCS-based composite scaffolds	745:789	Stable MGC- and MCS-based composite scaffolds were fabricated containing up to 5 wt% cryomilled DAT through initiation with long-wavelength ultraviolet light.
24331712	1	7	theme	natural	292:298	arg1	regeneration					312:323	natural soft tissue regeneration	292:323	natural soft tissue regeneration	292:323	An injectable tissue-engineered adipose substitute that could be used to deliver adipose-derived stem cells (ASCs), filling irregular defects and stimulating natural soft tissue regeneration, would have significant value in plastic and reconstructive surgery.
24331712	0	8	theme	tissue	82:87	arg1	engineering					89:99	soft tissue engineering	77:99	soft tissue engineering	77:99	Composite hydrogel scaffolds incorporating decellularized adipose tissue for soft tissue engineering with adipose-derived stem cells.
24331712	1	9	theme	adipose-derived	215:229	arg1	cells					236:240	adipose-derived stem cells	215:240	adipose-derived stem cells (ASCs)	215:247	An injectable tissue-engineered adipose substitute that could be used to deliver adipose-derived stem cells (ASCs), filling irregular defects and stimulating natural soft tissue regeneration, would have significant value in plastic and reconstructive surgery.
24331712	1	9	theme	adipose-derived	215:229	arg1	ASCs					243:246	ASCs	243:246	ASCs	243:246	An injectable tissue-engineered adipose substitute that could be used to deliver adipose-derived stem cells (ASCs), filling irregular defects and stimulating natural soft tissue regeneration, would have significant value in plastic and reconstructive surgery.
24331712	2	10	theme	adipose	575:581	arg1	DAT					591:593	DAT	591:593	DAT	591:593	With this focus, the primary aim of the current study was to characterize the response of human ASCs encapsulated within three-dimensional bioscaffolds incorporating decellularized adipose tissue (DAT) as a bioactive matrix within photo-cross-linkable methacrylated glycol chitosan (MGC) or methacrylated chondroitin sulphate (MCS) delivery vehicles.
24331712	2	10	theme	adipose	575:581	arg1	tissue					583:588	decellularized adipose tissue	560:588	decellularized adipose tissue (DAT)	560:594	With this focus, the primary aim of the current study was to characterize the response of human ASCs encapsulated within three-dimensional bioscaffolds incorporating decellularized adipose tissue (DAT) as a bioactive matrix within photo-cross-linkable methacrylated glycol chitosan (MGC) or methacrylated chondroitin sulphate (MCS) delivery vehicles.
24331712	8	11	theme	implant	1774:1780	arg1	integration					1782:1792	superior implant integration	1765:1792	superior implant integration	1765:1792	In vivo analysis in a subcutaneous Wistar rat model at 1, 4 and 12 weeks showed superior implant integration and adipogenesis in the MCS-based composites, with allogenic ASCs promoting cell infiltration, angiogenesis and ultimately, fat formation.
24331712	5	12	theme	in	1145:1146	arg1	studies					1167:1173	in vitro cell culture studies	1145:1173	in vitro cell culture studies	1145:1173	Through in vitro cell culture studies, human ASCs were assessed over 14 days in terms of viability, glycerol-3-phosphate dehydrogenase (GPDH) enzyme activity, adipogenic gene expression and intracellular lipid accumulation.
24331712	4	13	theme	3-D	956:958	arg1	microenvironment					960:975	the 3-D microenvironment	952:975	the 3-D microenvironment	952:975	The encapsulation strategy allows for tuning of the 3-D microenvironment and provides an effective method of cell delivery with high seeding efficiency and uniformity, which could be adapted as a minimally-invasive in situ approach.
24331712	1	14	theme	injectable	137:146	arg1	adipose					166:172	An injectable tissue-engineered adipose	134:172	An injectable tissue-engineered adipose	134:172	An injectable tissue-engineered adipose substitute that could be used to deliver adipose-derived stem cells (ASCs), filling irregular defects and stimulating natural soft tissue regeneration, would have significant value in plastic and reconstructive surgery.
24331712	7	15	theme	wt	1676:1677	arg1	DAT					1680:1682	5 wt% DAT	1674:1682	5 wt% DAT	1674:1682	The choice of hydrogel also influenced the cell response, with significantly higher viability and adipogenic differentiation observed in the MCS composites containing 5 wt% DAT.
24331712	5	16	theme	lipid	1341:1345	arg1	days					1209:1212	14 days	1206:1212	14 days in terms of viability	1206:1234	Through in vitro cell culture studies, human ASCs were assessed over 14 days in terms of viability, glycerol-3-phosphate dehydrogenase (GPDH) enzyme activity, adipogenic gene expression and intracellular lipid accumulation.
24331712	5	16	theme	lipid	1341:1345	arg1	accumulation					1347:1358	intracellular lipid accumulation	1327:1358	intracellular lipid accumulation	1327:1358	Through in vitro cell culture studies, human ASCs were assessed over 14 days in terms of viability, glycerol-3-phosphate dehydrogenase (GPDH) enzyme activity, adipogenic gene expression and intracellular lipid accumulation.
24331712	8	17	theme	MCS-based	1818:1826	arg1	composites					1828:1837	the MCS-based composites	1814:1837	the MCS-based composites	1814:1837	In vivo analysis in a subcutaneous Wistar rat model at 1, 4 and 12 weeks showed superior implant integration and adipogenesis in the MCS-based composites, with allogenic ASCs promoting cell infiltration, angiogenesis and ultimately, fat formation.
24331712	2	18	theme	three-dimensional	515:531	arg1	bioscaffolds					533:544	three-dimensional bioscaffolds	515:544	three-dimensional bioscaffolds incorporating decellularized adipose tissue (DAT)	515:594	With this focus, the primary aim of the current study was to characterize the response of human ASCs encapsulated within three-dimensional bioscaffolds incorporating decellularized adipose tissue (DAT) as a bioactive matrix within photo-cross-linkable methacrylated glycol chitosan (MGC) or methacrylated chondroitin sulphate (MCS) delivery vehicles.
24331712	3	19	theme	cryomilled	830:839	arg1	DAT					841:843	up to 5 wt% cryomilled DAT	818:843	up to 5 wt% cryomilled DAT	818:843	Stable MGC- and MCS-based composite scaffolds were fabricated containing up to 5 wt% cryomilled DAT through initiation with long-wavelength ultraviolet light.
24331712	0	20	theme	soft	77:80	arg1	engineering					89:99	soft tissue engineering	77:99	soft tissue engineering	77:99	Composite hydrogel scaffolds incorporating decellularized adipose tissue for soft tissue engineering with adipose-derived stem cells.
24331712	5	21	theme	human	1176:1180	arg1	ASCs					1182:1185	human ASCs	1176:1185	human ASCs	1176:1185	Through in vitro cell culture studies, human ASCs were assessed over 14 days in terms of viability, glycerol-3-phosphate dehydrogenase (GPDH) enzyme activity, adipogenic gene expression and intracellular lipid accumulation.
24331712	7	22	theme	MCS	1648:1650	arg1	composites					1652:1661	the MCS composites	1644:1661	the MCS composites containing 5 wt% DAT	1644:1682	The choice of hydrogel also influenced the cell response, with significantly higher viability and adipogenic differentiation observed in the MCS composites containing 5 wt% DAT.
24331712	5	23	theme	gene	1307:1310	arg1	expression					1312:1321	adipogenic gene expression	1296:1321	adipogenic gene expression	1296:1321	Through in vitro cell culture studies, human ASCs were assessed over 14 days in terms of viability, glycerol-3-phosphate dehydrogenase (GPDH) enzyme activity, adipogenic gene expression and intracellular lipid accumulation.
24331712	5	23	theme	gene	1307:1310	arg1	days					1209:1212	14 days	1206:1212	14 days in terms of viability	1206:1234	Through in vitro cell culture studies, human ASCs were assessed over 14 days in terms of viability, glycerol-3-phosphate dehydrogenase (GPDH) enzyme activity, adipogenic gene expression and intracellular lipid accumulation.
24331712	3	24	theme	ultraviolet	885:895	arg1	light					897:901	long-wavelength ultraviolet light	869:901	long-wavelength ultraviolet light	869:901	Stable MGC- and MCS-based composite scaffolds were fabricated containing up to 5 wt% cryomilled DAT through initiation with long-wavelength ultraviolet light.
24331712	8	25	dep	In	1685:1686	arg1	vivo					1688:1691	vivo	1688:1691	vivo	1688:1691	In vivo analysis in a subcutaneous Wistar rat model at 1, 4 and 12 weeks showed superior implant integration and adipogenesis in the MCS-based composites, with allogenic ASCs promoting cell infiltration, angiogenesis and ultimately, fat formation.
24331712	8	26	theme	allogenic	1845:1853	arg1	ASCs					1855:1858	allogenic ASCs	1845:1858	allogenic ASCs promoting cell infiltration, angiogenesis and ultimately, fat formation	1845:1930	In vivo analysis in a subcutaneous Wistar rat model at 1, 4 and 12 weeks showed superior implant integration and adipogenesis in the MCS-based composites, with allogenic ASCs promoting cell infiltration, angiogenesis and ultimately, fat formation.
24331712	4	27	theme	cell	1013:1016	arg1	delivery					1018:1025	cell delivery	1013:1025	cell delivery	1013:1025	The encapsulation strategy allows for tuning of the 3-D microenvironment and provides an effective method of cell delivery with high seeding efficiency and uniformity, which could be adapted as a minimally-invasive in situ approach.
24331712	2	28	theme	chondroitin	699:709	arg1	MCS					721:723	MCS	721:723	MCS	721:723	With this focus, the primary aim of the current study was to characterize the response of human ASCs encapsulated within three-dimensional bioscaffolds incorporating decellularized adipose tissue (DAT) as a bioactive matrix within photo-cross-linkable methacrylated glycol chitosan (MGC) or methacrylated chondroitin sulphate (MCS) delivery vehicles.
24331712	2	28	theme	chondroitin	699:709	arg1	sulphate					711:718	methacrylated chondroitin sulphate	685:718	methacrylated chondroitin sulphate (MCS)	685:724	With this focus, the primary aim of the current study was to characterize the response of human ASCs encapsulated within three-dimensional bioscaffolds incorporating decellularized adipose tissue (DAT) as a bioactive matrix within photo-cross-linkable methacrylated glycol chitosan (MGC) or methacrylated chondroitin sulphate (MCS) delivery vehicles.
24331712	0	29	theme	adipose-derived	106:120	arg1	cells					127:131	adipose-derived stem cells	106:131	adipose-derived stem cells	106:131	Composite hydrogel scaffolds incorporating decellularized adipose tissue for soft tissue engineering with adipose-derived stem cells.
24331712	5	30	theme	cell	1154:1157	arg1	studies					1167:1173	in vitro cell culture studies	1145:1173	in vitro cell culture studies	1145:1173	Through in vitro cell culture studies, human ASCs were assessed over 14 days in terms of viability, glycerol-3-phosphate dehydrogenase (GPDH) enzyme activity, adipogenic gene expression and intracellular lipid accumulation.
24331712	4	31	dep	in	1119:1120	arg1	situ					1122:1125	situ	1122:1125	situ	1122:1125	The encapsulation strategy allows for tuning of the 3-D microenvironment and provides an effective method of cell delivery with high seeding efficiency and uniformity, which could be adapted as a minimally-invasive in situ approach.
24331712	4	32	with	method	1003:1008	arg1	uniformity					1060:1069	uniformity	1060:1069	uniformity	1060:1069	The encapsulation strategy allows for tuning of the 3-D microenvironment and provides an effective method of cell delivery with high seeding efficiency and uniformity, which could be adapted as a minimally-invasive in situ approach.
24331712	4	32	with	method	1003:1008	arg1	approach					1127:1134	a minimally-invasive in situ approach	1098:1134	a minimally-invasive in situ approach	1098:1134	The encapsulation strategy allows for tuning of the 3-D microenvironment and provides an effective method of cell delivery with high seeding efficiency and uniformity, which could be adapted as a minimally-invasive in situ approach.
24331712	4	32	with	method	1003:1008	arg1	efficiency					1045:1054	high seeding efficiency	1032:1054	high seeding efficiency	1032:1054	The encapsulation strategy allows for tuning of the 3-D microenvironment and provides an effective method of cell delivery with high seeding efficiency and uniformity, which could be adapted as a minimally-invasive in situ approach.
24331712	2	33	theme	primary	415:421	arg1	aim					423:425	the primary aim	411:425	the primary aim of the current study	411:446	With this focus, the primary aim of the current study was to characterize the response of human ASCs encapsulated within three-dimensional bioscaffolds incorporating decellularized adipose tissue (DAT) as a bioactive matrix within photo-cross-linkable methacrylated glycol chitosan (MGC) or methacrylated chondroitin sulphate (MCS) delivery vehicles.
24331712	3	34	dep	wt	826:827	arg1	to					821:822	to	821:822	to	821:822	Stable MGC- and MCS-based composite scaffolds were fabricated containing up to 5 wt% cryomilled DAT through initiation with long-wavelength ultraviolet light.
24331712	4	35	theme	seeding	1037:1043	arg1	uniformity					1060:1069	uniformity	1060:1069	uniformity	1060:1069	The encapsulation strategy allows for tuning of the 3-D microenvironment and provides an effective method of cell delivery with high seeding efficiency and uniformity, which could be adapted as a minimally-invasive in situ approach.
24331712	4	35	theme	seeding	1037:1043	arg1	approach					1127:1134	a minimally-invasive in situ approach	1098:1134	a minimally-invasive in situ approach	1098:1134	The encapsulation strategy allows for tuning of the 3-D microenvironment and provides an effective method of cell delivery with high seeding efficiency and uniformity, which could be adapted as a minimally-invasive in situ approach.
24331712	4	35	theme	seeding	1037:1043	arg1	efficiency					1045:1054	high seeding efficiency	1032:1054	high seeding efficiency	1032:1054	The encapsulation strategy allows for tuning of the 3-D microenvironment and provides an effective method of cell delivery with high seeding efficiency and uniformity, which could be adapted as a minimally-invasive in situ approach.
24331712	0	36	theme	Composite	0:8	arg1	scaffolds					19:27	Composite hydrogel scaffolds	0:27	Composite hydrogel scaffolds	0:27	Composite hydrogel scaffolds incorporating decellularized adipose tissue for soft tissue engineering with adipose-derived stem cells.
24331712	1	37	theme	soft	300:303	arg1	regeneration					312:323	natural soft tissue regeneration	292:323	natural soft tissue regeneration	292:323	An injectable tissue-engineered adipose substitute that could be used to deliver adipose-derived stem cells (ASCs), filling irregular defects and stimulating natural soft tissue regeneration, would have significant value in plastic and reconstructive surgery.
24331712	2	38	theme	current	434:440	arg1	study					442:446	the current study	430:446	the current study	430:446	With this focus, the primary aim of the current study was to characterize the response of human ASCs encapsulated within three-dimensional bioscaffolds incorporating decellularized adipose tissue (DAT) as a bioactive matrix within photo-cross-linkable methacrylated glycol chitosan (MGC) or methacrylated chondroitin sulphate (MCS) delivery vehicles.
24331712	5	39	theme	viability	1226:1234	arg1	terms					1217:1221	terms	1217:1221	terms of viability	1217:1234	Through in vitro cell culture studies, human ASCs were assessed over 14 days in terms of viability, glycerol-3-phosphate dehydrogenase (GPDH) enzyme activity, adipogenic gene expression and intracellular lipid accumulation.
24331712	2	40	theme	human	484:488	arg1	ASCs					490:493	human ASCs	484:493	human ASCs encapsulated within three-dimensional bioscaffolds incorporating decellularized adipose tissue (DAT) as a bioactive matrix within photo-cross-linkable methacrylated glycol chitosan (MGC) or methacrylated chondroitin sulphate (MCS) delivery vehicles	484:742	With this focus, the primary aim of the current study was to characterize the response of human ASCs encapsulated within three-dimensional bioscaffolds incorporating decellularized adipose tissue (DAT) as a bioactive matrix within photo-cross-linkable methacrylated glycol chitosan (MGC) or methacrylated chondroitin sulphate (MCS) delivery vehicles.
24331712	0	41	theme	decellularized	43:56	arg1	tissue					66:71	decellularized adipose tissue	43:71	decellularized adipose tissue for soft tissue engineering with adipose-derived stem cells	43:131	Composite hydrogel scaffolds incorporating decellularized adipose tissue for soft tissue engineering with adipose-derived stem cells.
24331712	7	42	located	observed	1632:1639	arg2	viability					1591:1599	significantly higher viability	1570:1599	significantly higher viability	1570:1599	The choice of hydrogel also influenced the cell response, with significantly higher viability and adipogenic differentiation observed in the MCS composites containing 5 wt% DAT.
24331712	7	42	located	observed	1632:1639	arg1	composites					1652:1661	the MCS composites	1644:1661	the MCS composites containing 5 wt% DAT	1644:1682	The choice of hydrogel also influenced the cell response, with significantly higher viability and adipogenic differentiation observed in the MCS composites containing 5 wt% DAT.
24331712	1	43	theme	significant	337:347	arg1	value					349:353	significant value	337:353	significant value	337:353	An injectable tissue-engineered adipose substitute that could be used to deliver adipose-derived stem cells (ASCs), filling irregular defects and stimulating natural soft tissue regeneration, would have significant value in plastic and reconstructive surgery.
24331712	7	44	theme	cell	1550:1553	arg1	response					1555:1562	the cell response	1546:1562	the cell response	1546:1562	The choice of hydrogel also influenced the cell response, with significantly higher viability and adipogenic differentiation observed in the MCS composites containing 5 wt% DAT.
24331712	0	45	with	tissue	66:71	arg1	cells					127:131	adipose-derived stem cells	106:131	adipose-derived stem cells	106:131	Composite hydrogel scaffolds incorporating decellularized adipose tissue for soft tissue engineering with adipose-derived stem cells.
24331712	8	46	theme	subcutaneous	1707:1718	arg1	model					1731:1735	a subcutaneous Wistar rat model	1705:1735	a subcutaneous Wistar rat model	1705:1735	In vivo analysis in a subcutaneous Wistar rat model at 1, 4 and 12 weeks showed superior implant integration and adipogenesis in the MCS-based composites, with allogenic ASCs promoting cell infiltration, angiogenesis and ultimately, fat formation.
24331712	4	47	theme	in	1119:1120	arg1	uniformity					1060:1069	uniformity	1060:1069	uniformity	1060:1069	The encapsulation strategy allows for tuning of the 3-D microenvironment and provides an effective method of cell delivery with high seeding efficiency and uniformity, which could be adapted as a minimally-invasive in situ approach.
24331712	4	47	theme	in	1119:1120	arg1	approach					1127:1134	a minimally-invasive in situ approach	1098:1134	a minimally-invasive in situ approach	1098:1134	The encapsulation strategy allows for tuning of the 3-D microenvironment and provides an effective method of cell delivery with high seeding efficiency and uniformity, which could be adapted as a minimally-invasive in situ approach.
24331712	4	47	theme	in	1119:1120	arg1	efficiency					1045:1054	high seeding efficiency	1032:1054	high seeding efficiency	1032:1054	The encapsulation strategy allows for tuning of the 3-D microenvironment and provides an effective method of cell delivery with high seeding efficiency and uniformity, which could be adapted as a minimally-invasive in situ approach.
24331712	3	48	theme	MGC-	752:755	arg1	scaffolds					781:789	Stable MGC- and MCS-based composite scaffolds	745:789	Stable MGC- and MCS-based composite scaffolds	745:789	Stable MGC- and MCS-based composite scaffolds were fabricated containing up to 5 wt% cryomilled DAT through initiation with long-wavelength ultraviolet light.
24331712	5	49	from	days	1209:1212	arg1	terms					1217:1221	terms	1217:1221	terms of viability	1217:1234	Through in vitro cell culture studies, human ASCs were assessed over 14 days in terms of viability, glycerol-3-phosphate dehydrogenase (GPDH) enzyme activity, adipogenic gene expression and intracellular lipid accumulation.
24331712	8	50	theme	rat	1727:1729	arg1	model					1731:1735	a subcutaneous Wistar rat model	1705:1735	a subcutaneous Wistar rat model	1705:1735	In vivo analysis in a subcutaneous Wistar rat model at 1, 4 and 12 weeks showed superior implant integration and adipogenesis in the MCS-based composites, with allogenic ASCs promoting cell infiltration, angiogenesis and ultimately, fat formation.
24331712	5	51	theme	glycerol-3-phosphate	1237:1256	arg1	GPDH					1273:1276	GPDH	1273:1276	GPDH	1273:1276	Through in vitro cell culture studies, human ASCs were assessed over 14 days in terms of viability, glycerol-3-phosphate dehydrogenase (GPDH) enzyme activity, adipogenic gene expression and intracellular lipid accumulation.
24331712	5	51	theme	glycerol-3-phosphate	1237:1256	arg1	dehydrogenase					1258:1270	glycerol-3-phosphate dehydrogenase	1237:1270	glycerol-3-phosphate dehydrogenase (GPDH) enzyme activity	1237:1293	Through in vitro cell culture studies, human ASCs were assessed over 14 days in terms of viability, glycerol-3-phosphate dehydrogenase (GPDH) enzyme activity, adipogenic gene expression and intracellular lipid accumulation.
24331712	7	52	theme	hydrogel	1521:1528	arg1	choice					1511:1516	The choice	1507:1516	The choice of hydrogel	1507:1528	The choice of hydrogel also influenced the cell response, with significantly higher viability and adipogenic differentiation observed in the MCS composites containing 5 wt% DAT.
24331712	2	53	theme	methacrylated	646:658	arg1	MGC					677:679	MGC	677:679	MGC	677:679	With this focus, the primary aim of the current study was to characterize the response of human ASCs encapsulated within three-dimensional bioscaffolds incorporating decellularized adipose tissue (DAT) as a bioactive matrix within photo-cross-linkable methacrylated glycol chitosan (MGC) or methacrylated chondroitin sulphate (MCS) delivery vehicles.
24331712	2	53	theme	methacrylated	646:658	arg1	chitosan					667:674	photo-cross-linkable methacrylated glycol chitosan	625:674	photo-cross-linkable methacrylated glycol chitosan (MGC)	625:680	With this focus, the primary aim of the current study was to characterize the response of human ASCs encapsulated within three-dimensional bioscaffolds incorporating decellularized adipose tissue (DAT) as a bioactive matrix within photo-cross-linkable methacrylated glycol chitosan (MGC) or methacrylated chondroitin sulphate (MCS) delivery vehicles.
24331712	8	54	theme	fat	1918:1920	arg1	formation					1922:1930	fat formation	1918:1930	fat formation	1918:1930	In vivo analysis in a subcutaneous Wistar rat model at 1, 4 and 12 weeks showed superior implant integration and adipogenesis in the MCS-based composites, with allogenic ASCs promoting cell infiltration, angiogenesis and ultimately, fat formation.
24331712	3	55	theme	MCS-based	761:769	arg1	scaffolds					781:789	Stable MGC- and MCS-based composite scaffolds	745:789	Stable MGC- and MCS-based composite scaffolds	745:789	Stable MGC- and MCS-based composite scaffolds were fabricated containing up to 5 wt% cryomilled DAT through initiation with long-wavelength ultraviolet light.
24331712	2	56	dep	chitosan	667:674	arg1	vehicles					735:742	delivery vehicles	726:742	delivery vehicles	726:742	With this focus, the primary aim of the current study was to characterize the response of human ASCs encapsulated within three-dimensional bioscaffolds incorporating decellularized adipose tissue (DAT) as a bioactive matrix within photo-cross-linkable methacrylated glycol chitosan (MGC) or methacrylated chondroitin sulphate (MCS) delivery vehicles.
24331712	4	57	theme	encapsulation	908:920	arg1	strategy					922:929	The encapsulation strategy	904:929	The encapsulation strategy	904:929	The encapsulation strategy allows for tuning of the 3-D microenvironment and provides an effective method of cell delivery with high seeding efficiency and uniformity, which could be adapted as a minimally-invasive in situ approach.
24331712	1	58	theme	stem	231:234	arg1	cells					236:240	adipose-derived stem cells	215:240	adipose-derived stem cells (ASCs)	215:247	An injectable tissue-engineered adipose substitute that could be used to deliver adipose-derived stem cells (ASCs), filling irregular defects and stimulating natural soft tissue regeneration, would have significant value in plastic and reconstructive surgery.
24331712	1	58	theme	stem	231:234	arg1	ASCs					243:246	ASCs	243:246	ASCs	243:246	An injectable tissue-engineered adipose substitute that could be used to deliver adipose-derived stem cells (ASCs), filling irregular defects and stimulating natural soft tissue regeneration, would have significant value in plastic and reconstructive surgery.
24331712	3	59	dep	DAT	841:843	arg1	%					828:828	%	828:828	%	828:828	Stable MGC- and MCS-based composite scaffolds were fabricated containing up to 5 wt% cryomilled DAT through initiation with long-wavelength ultraviolet light.
24331712	8	60	theme	superior	1765:1772	arg1	integration					1782:1792	superior implant integration	1765:1792	superior implant integration	1765:1792	In vivo analysis in a subcutaneous Wistar rat model at 1, 4 and 12 weeks showed superior implant integration and adipogenesis in the MCS-based composites, with allogenic ASCs promoting cell infiltration, angiogenesis and ultimately, fat formation.
24331712	7	61	theme	%	1678:1678	arg1	DAT					1680:1682	5 wt% DAT	1674:1682	5 wt% DAT	1674:1682	The choice of hydrogel also influenced the cell response, with significantly higher viability and adipogenic differentiation observed in the MCS composites containing 5 wt% DAT.
24331712	4	62	theme	effective	993:1001	arg1	method					1003:1008	an effective method	990:1008	an effective method of cell delivery with high seeding efficiency and uniformity, which could be adapted as a minimally-invasive in situ approach	990:1134	The encapsulation strategy allows for tuning of the 3-D microenvironment and provides an effective method of cell delivery with high seeding efficiency and uniformity, which could be adapted as a minimally-invasive in situ approach.
24331712	0	63	theme	stem	122:125	arg1	cells					127:131	adipose-derived stem cells	106:131	adipose-derived stem cells	106:131	Composite hydrogel scaffolds incorporating decellularized adipose tissue for soft tissue engineering with adipose-derived stem cells.
24331712	8	64	from	analysis	1693:1700	arg1	model					1731:1735	a subcutaneous Wistar rat model	1705:1735	a subcutaneous Wistar rat model	1705:1735	In vivo analysis in a subcutaneous Wistar rat model at 1, 4 and 12 weeks showed superior implant integration and adipogenesis in the MCS-based composites, with allogenic ASCs promoting cell infiltration, angiogenesis and ultimately, fat formation.
24331712	2	65	theme	bioactive	601:609	arg1	matrix					611:616	a bioactive matrix	599:616	a bioactive matrix	599:616	With this focus, the primary aim of the current study was to characterize the response of human ASCs encapsulated within three-dimensional bioscaffolds incorporating decellularized adipose tissue (DAT) as a bioactive matrix within photo-cross-linkable methacrylated glycol chitosan (MGC) or methacrylated chondroitin sulphate (MCS) delivery vehicles.
24331712	5	66	theme	intracellular	1327:1339	arg1	days					1209:1212	14 days	1206:1212	14 days in terms of viability	1206:1234	Through in vitro cell culture studies, human ASCs were assessed over 14 days in terms of viability, glycerol-3-phosphate dehydrogenase (GPDH) enzyme activity, adipogenic gene expression and intracellular lipid accumulation.
24331712	5	66	theme	intracellular	1327:1339	arg1	accumulation					1347:1358	intracellular lipid accumulation	1327:1358	intracellular lipid accumulation	1327:1358	Through in vitro cell culture studies, human ASCs were assessed over 14 days in terms of viability, glycerol-3-phosphate dehydrogenase (GPDH) enzyme activity, adipogenic gene expression and intracellular lipid accumulation.
24331712	3	67	theme	5	824:824	arg1	wt					826:827	wt	826:827	wt	826:827	Stable MGC- and MCS-based composite scaffolds were fabricated containing up to 5 wt% cryomilled DAT through initiation with long-wavelength ultraviolet light.
24331712	1	68	theme	tissue-engineered	148:164	arg1	adipose					166:172	An injectable tissue-engineered adipose	134:172	An injectable tissue-engineered adipose	134:172	An injectable tissue-engineered adipose substitute that could be used to deliver adipose-derived stem cells (ASCs), filling irregular defects and stimulating natural soft tissue regeneration, would have significant value in plastic and reconstructive surgery.
24331712	4	69	theme	microenvironment	960:975	arg1	tuning					942:947	tuning	942:947	tuning of the 3-D microenvironment	942:975	The encapsulation strategy allows for tuning of the 3-D microenvironment and provides an effective method of cell delivery with high seeding efficiency and uniformity, which could be adapted as a minimally-invasive in situ approach.
24331712	6	70	theme	ASC	1448:1450	arg1	viability					1452:1460	ASC viability	1448:1460	ASC viability	1448:1460	In all of the composites, the DAT functioned as a cell-supportive matrix that enhanced ASC viability, retention and adipogenesis within the gels.
24331712	4	71	theme	high	1032:1035	arg1	uniformity					1060:1069	uniformity	1060:1069	uniformity	1060:1069	The encapsulation strategy allows for tuning of the 3-D microenvironment and provides an effective method of cell delivery with high seeding efficiency and uniformity, which could be adapted as a minimally-invasive in situ approach.
24331712	4	71	theme	high	1032:1035	arg1	approach					1127:1134	a minimally-invasive in situ approach	1098:1134	a minimally-invasive in situ approach	1098:1134	The encapsulation strategy allows for tuning of the 3-D microenvironment and provides an effective method of cell delivery with high seeding efficiency and uniformity, which could be adapted as a minimally-invasive in situ approach.
24331712	4	71	theme	high	1032:1035	arg1	efficiency					1045:1054	high seeding efficiency	1032:1054	high seeding efficiency	1032:1054	The encapsulation strategy allows for tuning of the 3-D microenvironment and provides an effective method of cell delivery with high seeding efficiency and uniformity, which could be adapted as a minimally-invasive in situ approach.
24331712	5	72	theme	adipogenic	1296:1305	arg1	expression					1312:1321	adipogenic gene expression	1296:1321	adipogenic gene expression	1296:1321	Through in vitro cell culture studies, human ASCs were assessed over 14 days in terms of viability, glycerol-3-phosphate dehydrogenase (GPDH) enzyme activity, adipogenic gene expression and intracellular lipid accumulation.
24331712	5	72	theme	adipogenic	1296:1305	arg1	days					1209:1212	14 days	1206:1212	14 days in terms of viability	1206:1234	Through in vitro cell culture studies, human ASCs were assessed over 14 days in terms of viability, glycerol-3-phosphate dehydrogenase (GPDH) enzyme activity, adipogenic gene expression and intracellular lipid accumulation.
24331712	3	73	with	initiation	853:862	arg1	light					897:901	long-wavelength ultraviolet light	869:901	long-wavelength ultraviolet light	869:901	Stable MGC- and MCS-based composite scaffolds were fabricated containing up to 5 wt% cryomilled DAT through initiation with long-wavelength ultraviolet light.
24331712	2	74	theme	ASCs	490:493	arg1	response					472:479	the response	468:479	the response of human ASCs encapsulated within three-dimensional bioscaffolds incorporating decellularized adipose tissue (DAT) as a bioactive matrix within photo-cross-linkable methacrylated glycol chitosan (MGC) or methacrylated chondroitin sulphate (MCS) delivery vehicles	468:742	With this focus, the primary aim of the current study was to characterize the response of human ASCs encapsulated within three-dimensional bioscaffolds incorporating decellularized adipose tissue (DAT) as a bioactive matrix within photo-cross-linkable methacrylated glycol chitosan (MGC) or methacrylated chondroitin sulphate (MCS) delivery vehicles.
24331712	6	75	theme	cell-supportive	1411:1425	arg1	matrix					1427:1432	a cell-supportive matrix	1409:1432	a cell-supportive matrix that enhanced ASC viability, retention and adipogenesis within the gels	1409:1504	In all of the composites, the DAT functioned as a cell-supportive matrix that enhanced ASC viability, retention and adipogenesis within the gels.
24331712	6	75	theme	cell-supportive	1411:1425	arg1	DAT					1391:1393	the DAT	1387:1393	the DAT	1387:1393	In all of the composites, the DAT functioned as a cell-supportive matrix that enhanced ASC viability, retention and adipogenesis within the gels.
24331712	1	76	theme	reconstructive	370:383	arg1	surgery					385:391	reconstructive surgery	370:391	reconstructive surgery	370:391	An injectable tissue-engineered adipose substitute that could be used to deliver adipose-derived stem cells (ASCs), filling irregular defects and stimulating natural soft tissue regeneration, would have significant value in plastic and reconstructive surgery.
24331712	7	77	theme	adipogenic	1605:1614	arg1	differentiation					1616:1630	adipogenic differentiation	1605:1630	adipogenic differentiation	1605:1630	The choice of hydrogel also influenced the cell response, with significantly higher viability and adipogenic differentiation observed in the MCS composites containing 5 wt% DAT.
24331712	2	78	theme	decellularized	560:573	arg1	DAT					591:593	DAT	591:593	DAT	591:593	With this focus, the primary aim of the current study was to characterize the response of human ASCs encapsulated within three-dimensional bioscaffolds incorporating decellularized adipose tissue (DAT) as a bioactive matrix within photo-cross-linkable methacrylated glycol chitosan (MGC) or methacrylated chondroitin sulphate (MCS) delivery vehicles.
24331712	2	78	theme	decellularized	560:573	arg1	tissue					583:588	decellularized adipose tissue	560:588	decellularized adipose tissue (DAT)	560:594	With this focus, the primary aim of the current study was to characterize the response of human ASCs encapsulated within three-dimensional bioscaffolds incorporating decellularized adipose tissue (DAT) as a bioactive matrix within photo-cross-linkable methacrylated glycol chitosan (MGC) or methacrylated chondroitin sulphate (MCS) delivery vehicles.
24331712	2	79	theme	methacrylated	685:697	arg1	MCS					721:723	MCS	721:723	MCS	721:723	With this focus, the primary aim of the current study was to characterize the response of human ASCs encapsulated within three-dimensional bioscaffolds incorporating decellularized adipose tissue (DAT) as a bioactive matrix within photo-cross-linkable methacrylated glycol chitosan (MGC) or methacrylated chondroitin sulphate (MCS) delivery vehicles.
24331712	2	79	theme	methacrylated	685:697	arg1	sulphate					711:718	methacrylated chondroitin sulphate	685:718	methacrylated chondroitin sulphate (MCS)	685:724	With this focus, the primary aim of the current study was to characterize the response of human ASCs encapsulated within three-dimensional bioscaffolds incorporating decellularized adipose tissue (DAT) as a bioactive matrix within photo-cross-linkable methacrylated glycol chitosan (MGC) or methacrylated chondroitin sulphate (MCS) delivery vehicles.
24331712	3	80	theme	long-wavelength	869:883	arg1	light					897:901	long-wavelength ultraviolet light	869:901	long-wavelength ultraviolet light	869:901	Stable MGC- and MCS-based composite scaffolds were fabricated containing up to 5 wt% cryomilled DAT through initiation with long-wavelength ultraviolet light.
24331712	5	81	theme	culture	1159:1165	arg1	studies					1167:1173	in vitro cell culture studies	1145:1173	in vitro cell culture studies	1145:1173	Through in vitro cell culture studies, human ASCs were assessed over 14 days in terms of viability, glycerol-3-phosphate dehydrogenase (GPDH) enzyme activity, adipogenic gene expression and intracellular lipid accumulation.
24331712	4	82	theme	delivery	1018:1025	arg1	method					1003:1008	an effective method	990:1008	an effective method of cell delivery with high seeding efficiency and uniformity, which could be adapted as a minimally-invasive in situ approach	990:1134	The encapsulation strategy allows for tuning of the 3-D microenvironment and provides an effective method of cell delivery with high seeding efficiency and uniformity, which could be adapted as a minimally-invasive in situ approach.
24331712	1	83	link	adipose-derived	215:229	arg1	cells					236:240	adipose-derived stem cells	215:240	adipose-derived stem cells (ASCs)	215:247	An injectable tissue-engineered adipose substitute that could be used to deliver adipose-derived stem cells (ASCs), filling irregular defects and stimulating natural soft tissue regeneration, would have significant value in plastic and reconstructive surgery.
24331712	1	83	link	adipose-derived	215:229	arg1	ASCs					243:246	ASCs	243:246	ASCs	243:246	An injectable tissue-engineered adipose substitute that could be used to deliver adipose-derived stem cells (ASCs), filling irregular defects and stimulating natural soft tissue regeneration, would have significant value in plastic and reconstructive surgery.
24331712	2	84	theme	delivery	726:733	arg1	vehicles					735:742	delivery vehicles	726:742	delivery vehicles	726:742	With this focus, the primary aim of the current study was to characterize the response of human ASCs encapsulated within three-dimensional bioscaffolds incorporating decellularized adipose tissue (DAT) as a bioactive matrix within photo-cross-linkable methacrylated glycol chitosan (MGC) or methacrylated chondroitin sulphate (MCS) delivery vehicles.
24331712	0	85	theme	hydrogel	10:17	arg1	scaffolds					19:27	Composite hydrogel scaffolds	0:27	Composite hydrogel scaffolds	0:27	Composite hydrogel scaffolds incorporating decellularized adipose tissue for soft tissue engineering with adipose-derived stem cells.
24331712	3	86	dep	%	828:828	arg1	wt					826:827	wt	826:827	wt	826:827	Stable MGC- and MCS-based composite scaffolds were fabricated containing up to 5 wt% cryomilled DAT through initiation with long-wavelength ultraviolet light.
24331712	8	87	theme	cell	1870:1873	arg1	infiltration					1875:1886	cell infiltration	1870:1886	cell infiltration	1870:1886	In vivo analysis in a subcutaneous Wistar rat model at 1, 4 and 12 weeks showed superior implant integration and adipogenesis in the MCS-based composites, with allogenic ASCs promoting cell infiltration, angiogenesis and ultimately, fat formation.
24331712	1	88	theme	tissue	305:310	arg1	regeneration					312:323	natural soft tissue regeneration	292:323	natural soft tissue regeneration	292:323	An injectable tissue-engineered adipose substitute that could be used to deliver adipose-derived stem cells (ASCs), filling irregular defects and stimulating natural soft tissue regeneration, would have significant value in plastic and reconstructive surgery.
24331712	2	89	theme	study	442:446	arg1	aim					423:425	the primary aim	411:425	the primary aim of the current study	411:446	With this focus, the primary aim of the current study was to characterize the response of human ASCs encapsulated within three-dimensional bioscaffolds incorporating decellularized adipose tissue (DAT) as a bioactive matrix within photo-cross-linkable methacrylated glycol chitosan (MGC) or methacrylated chondroitin sulphate (MCS) delivery vehicles.
24331712	0	90	theme	adipose	58:64	arg1	tissue					66:71	decellularized adipose tissue	43:71	decellularized adipose tissue for soft tissue engineering with adipose-derived stem cells	43:131	Composite hydrogel scaffolds incorporating decellularized adipose tissue for soft tissue engineering with adipose-derived stem cells.
24331712	7	91	theme	higher	1584:1589	arg1	viability					1591:1599	significantly higher viability	1570:1599	significantly higher viability	1570:1599	The choice of hydrogel also influenced the cell response, with significantly higher viability and adipogenic differentiation observed in the MCS composites containing 5 wt% DAT.
24331712	5	92	dep	in	1145:1146	arg1	vitro					1148:1152	vitro	1148:1152	vitro	1148:1152	Through in vitro cell culture studies, human ASCs were assessed over 14 days in terms of viability, glycerol-3-phosphate dehydrogenase (GPDH) enzyme activity, adipogenic gene expression and intracellular lipid accumulation.
24331712	8	93	theme	Wistar	1720:1725	arg1	model					1731:1735	a subcutaneous Wistar rat model	1705:1735	a subcutaneous Wistar rat model	1705:1735	In vivo analysis in a subcutaneous Wistar rat model at 1, 4 and 12 weeks showed superior implant integration and adipogenesis in the MCS-based composites, with allogenic ASCs promoting cell infiltration, angiogenesis and ultimately, fat formation.
24331712	5	94	theme	enzyme	1279:1284	arg1	days					1209:1212	14 days	1206:1212	14 days in terms of viability	1206:1234	Through in vitro cell culture studies, human ASCs were assessed over 14 days in terms of viability, glycerol-3-phosphate dehydrogenase (GPDH) enzyme activity, adipogenic gene expression and intracellular lipid accumulation.
24331712	5	94	theme	enzyme	1279:1284	arg1	activity					1286:1293	glycerol-3-phosphate dehydrogenase (GPDH) enzyme activity	1237:1293	glycerol-3-phosphate dehydrogenase (GPDH) enzyme activity	1237:1293	Through in vitro cell culture studies, human ASCs were assessed over 14 days in terms of viability, glycerol-3-phosphate dehydrogenase (GPDH) enzyme activity, adipogenic gene expression and intracellular lipid accumulation.
24331712	7	95	contain	containing	1663:1672	arg2	DAT					1680:1682	5 wt% DAT	1674:1682	5 wt% DAT	1674:1682	The choice of hydrogel also influenced the cell response, with significantly higher viability and adipogenic differentiation observed in the MCS composites containing 5 wt% DAT.
24331712	7	95	contain	containing	1663:1672	arg1	composites					1652:1661	the MCS composites	1644:1661	the MCS composites containing 5 wt% DAT	1644:1682	The choice of hydrogel also influenced the cell response, with significantly higher viability and adipogenic differentiation observed in the MCS composites containing 5 wt% DAT.
24331712	3	96	theme	Stable	745:750	arg1	scaffolds					781:789	Stable MGC- and MCS-based composite scaffolds	745:789	Stable MGC- and MCS-based composite scaffolds	745:789	Stable MGC- and MCS-based composite scaffolds were fabricated containing up to 5 wt% cryomilled DAT through initiation with long-wavelength ultraviolet light.
24331712	8	97	from	weeks	1752:1756	arg1	analysis					1693:1700	In vivo analysis	1685:1700	In vivo analysis in a subcutaneous Wistar rat model at 1, 4 and 12 weeks	1685:1756	In vivo analysis in a subcutaneous Wistar rat model at 1, 4 and 12 weeks showed superior implant integration and adipogenesis in the MCS-based composites, with allogenic ASCs promoting cell infiltration, angiogenesis and ultimately, fat formation.
24331712	1	98	theme	irregular	258:266	arg1	defects					268:274	irregular defects	258:274	irregular defects	258:274	An injectable tissue-engineered adipose substitute that could be used to deliver adipose-derived stem cells (ASCs), filling irregular defects and stimulating natural soft tissue regeneration, would have significant value in plastic and reconstructive surgery.
26116390	2	0	dep	values	276:281	arg1	to					260:261	to	260:261	to	260:261	The nitrogen to carbon (N/C) values and FTIR spectra were used to compare the samples based on their composition.
26116390	6	1	theme	chitosan	949:956	arg1	β-D-glucans					960:970	chitosan – β-D-glucans	949:970	chitosan – β-D-glucans	949:970	Chitosan lactates, chitosan – β-D-glucans and chitin – β-D-glucans of high and low amounts of chitin were successfully discriminated from the reference polysaccharides and from each other.
26116390	3	2	theme	ratio	471:475	arg1	chit					479:482	chit	479:482	chit	479:482	It was found that the N/C ratio correlates well with deacetylation degree (DD) of chitosans and chitin/glucan ratio R(chit) of fungal chitin – β-D-glucan complexes with the exception of some samples having significant nitrogen and/or carbon admixtures.
26116390	3	2	theme	ratio	471:475	arg1	R					477:477	chitin/glucan ratio R	457:477	chitin/glucan ratio R(chit)	457:483	It was found that the N/C ratio correlates well with deacetylation degree (DD) of chitosans and chitin/glucan ratio R(chit) of fungal chitin – β-D-glucan complexes with the exception of some samples having significant nitrogen and/or carbon admixtures.
26116390	4	3	theme	chitosan	719:726	arg1	contribution					729:740	the chitin (chitosan) contribution	707:740	the chitin (chitosan) contribution to fungal polysaccharide preparations	707:778	FTIR spectroscopy was indicative for the N-acetylation of chitins (chitosans) as well as for the chitin (chitosan) contribution to fungal polysaccharide preparations.
26116390	3	4	theme	–	502:502	arg1	complexes					515:523	fungal chitin – β-D-glucan complexes	488:523	fungal chitin – β-D-glucan complexes with the exception of some samples having significant nitrogen and/or carbon admixtures	488:611	It was found that the N/C ratio correlates well with deacetylation degree (DD) of chitosans and chitin/glucan ratio R(chit) of fungal chitin – β-D-glucan complexes with the exception of some samples having significant nitrogen and/or carbon admixtures.
26116390	7	5	theme	N/C	1156:1158	arg1	ratio					1160:1164	the N/C ratio	1152:1164	the N/C ratio	1152:1164	The proposed procedures based on the N/C ratio and multivariate analyses of FTIR spectra may be used in screening fungal polysaccharide preparations.
26116390	3	6	theme	fungal	488:493	arg1	complexes					515:523	fungal chitin – β-D-glucan complexes	488:523	fungal chitin – β-D-glucan complexes with the exception of some samples having significant nitrogen and/or carbon admixtures	488:611	It was found that the N/C ratio correlates well with deacetylation degree (DD) of chitosans and chitin/glucan ratio R(chit) of fungal chitin – β-D-glucan complexes with the exception of some samples having significant nitrogen and/or carbon admixtures.
26116390	7	7	theme	FTIR	1195:1198	arg1	spectra					1200:1206	FTIR spectra	1195:1206	FTIR spectra	1195:1206	The proposed procedures based on the N/C ratio and multivariate analyses of FTIR spectra may be used in screening fungal polysaccharide preparations.
26116390	3	8	theme	chitin/glucan	457:469	arg1	ratio					471:475	chitin/glucan ratio	457:475	chitin/glucan ratio R(chit)	457:483	It was found that the N/C ratio correlates well with deacetylation degree (DD) of chitosans and chitin/glucan ratio R(chit) of fungal chitin – β-D-glucan complexes with the exception of some samples having significant nitrogen and/or carbon admixtures.
26116390	5	9	theme	FTIR	810:813	arg1	data					815:818	the FTIR data	806:818	the FTIR data (HCA, PCA)	806:829	Multivariate analyses of the FTIR data (HCA, PCA) discriminated samples and reference materials into several clusters depending on their similarity.
26116390	3	10	theme	chitosans	443:451	arg1	degree					428:433	deacetylation degree	414:433	deacetylation degree (DD) of chitosans	414:451	It was found that the N/C ratio correlates well with deacetylation degree (DD) of chitosans and chitin/glucan ratio R(chit) of fungal chitin – β-D-glucan complexes with the exception of some samples having significant nitrogen and/or carbon admixtures.
26116390	3	10	theme	chitosans	443:451	arg1	chit					479:482	chit	479:482	chit	479:482	It was found that the N/C ratio correlates well with deacetylation degree (DD) of chitosans and chitin/glucan ratio R(chit) of fungal chitin – β-D-glucan complexes with the exception of some samples having significant nitrogen and/or carbon admixtures.
26116390	3	10	theme	chitosans	443:451	arg1	R					477:477	chitin/glucan ratio R	457:477	chitin/glucan ratio R(chit)	457:483	It was found that the N/C ratio correlates well with deacetylation degree (DD) of chitosans and chitin/glucan ratio R(chit) of fungal chitin – β-D-glucan complexes with the exception of some samples having significant nitrogen and/or carbon admixtures.
26116390	3	10	theme	chitosans	443:451	arg1	DD					436:437	DD	436:437	DD	436:437	It was found that the N/C ratio correlates well with deacetylation degree (DD) of chitosans and chitin/glucan ratio R(chit) of fungal chitin – β-D-glucan complexes with the exception of some samples having significant nitrogen and/or carbon admixtures.
26116390	2	11	theme	N/C	271:273	arg1	values					276:281	The nitrogen to carbon (N/C) values	247:281	The nitrogen to carbon (N/C) values	247:281	The nitrogen to carbon (N/C) values and FTIR spectra were used to compare the samples based on their composition.
26116390	1	12	theme	FTIR	165:168	arg1	spectroscopy					170:181	FTIR spectroscopy	165:181	FTIR spectroscopy	165:181	A set of fungal polysaccharide samples was characterised by elemental analysis and FTIR spectroscopy and compared with reference chitins, chitosans and β-D-glucans.
26116390	6	13	theme	Chitosan	930:937	arg1	lactates					939:946	Chitosan lactates	930:946	Chitosan lactates	930:946	Chitosan lactates, chitosan – β-D-glucans and chitin – β-D-glucans of high and low amounts of chitin were successfully discriminated from the reference polysaccharides and from each other.
26116390	2	14	used	used	305:308	arg2	spectra					292:298	FTIR spectra	287:298	FTIR spectra	287:298	The nitrogen to carbon (N/C) values and FTIR spectra were used to compare the samples based on their composition.
26116390	2	14	used	used	305:308	arg2	values					276:281	The nitrogen to carbon (N/C) values	247:281	The nitrogen to carbon (N/C) values	247:281	The nitrogen to carbon (N/C) values and FTIR spectra were used to compare the samples based on their composition.
26116390	4	15	theme	chitin	711:716	arg1	contribution					729:740	the chitin (chitosan) contribution	707:740	the chitin (chitosan) contribution to fungal polysaccharide preparations	707:778	FTIR spectroscopy was indicative for the N-acetylation of chitins (chitosans) as well as for the chitin (chitosan) contribution to fungal polysaccharide preparations.
26116390	6	16	theme	chitin	1024:1029	arg1	chitin					1024:1029	chitin	1024:1029	chitin	1024:1029	Chitosan lactates, chitosan – β-D-glucans and chitin – β-D-glucans of high and low amounts of chitin were successfully discriminated from the reference polysaccharides and from each other.
26116390	6	16	theme	chitin	1024:1029	arg1	amounts					1013:1019	high and low amounts	1000:1019	high and low amounts of chitin	1000:1029	Chitosan lactates, chitosan – β-D-glucans and chitin – β-D-glucans of high and low amounts of chitin were successfully discriminated from the reference polysaccharides and from each other.
26116390	5	17	theme	data	815:818	arg1	analyses					794:801	Multivariate analyses	781:801	Multivariate analyses of the FTIR data (HCA, PCA)	781:829	Multivariate analyses of the FTIR data (HCA, PCA) discriminated samples and reference materials into several clusters depending on their similarity.
26116390	7	18	theme	proposed	1123:1130	arg1	procedures					1132:1141	The proposed procedures	1119:1141	The proposed procedures based on the N/C ratio and multivariate analyses of FTIR spectra	1119:1206	The proposed procedures based on the N/C ratio and multivariate analyses of FTIR spectra may be used in screening fungal polysaccharide preparations.
26116390	7	19	theme	multivariate	1170:1181	arg1	analyses					1183:1190	multivariate analyses	1170:1190	multivariate analyses of FTIR spectra	1170:1206	The proposed procedures based on the N/C ratio and multivariate analyses of FTIR spectra may be used in screening fungal polysaccharide preparations.
26116390	3	20	theme	β-D-glucan	504:513	arg1	complexes					515:523	fungal chitin – β-D-glucan complexes	488:523	fungal chitin – β-D-glucan complexes with the exception of some samples having significant nitrogen and/or carbon admixtures	488:611	It was found that the N/C ratio correlates well with deacetylation degree (DD) of chitosans and chitin/glucan ratio R(chit) of fungal chitin – β-D-glucan complexes with the exception of some samples having significant nitrogen and/or carbon admixtures.
26116390	6	21	theme	low	1009:1011	arg1	chitin					1024:1029	chitin	1024:1029	chitin	1024:1029	Chitosan lactates, chitosan – β-D-glucans and chitin – β-D-glucans of high and low amounts of chitin were successfully discriminated from the reference polysaccharides and from each other.
26116390	6	21	theme	low	1009:1011	arg1	amounts					1013:1019	high and low amounts	1000:1019	high and low amounts of chitin	1000:1029	Chitosan lactates, chitosan – β-D-glucans and chitin – β-D-glucans of high and low amounts of chitin were successfully discriminated from the reference polysaccharides and from each other.
26116390	6	22	theme	–	958:958	arg1	β-D-glucans					960:970	chitosan – β-D-glucans	949:970	chitosan – β-D-glucans	949:970	Chitosan lactates, chitosan – β-D-glucans and chitin – β-D-glucans of high and low amounts of chitin were successfully discriminated from the reference polysaccharides and from each other.
26116390	4	23	theme	FTIR	614:617	arg1	spectroscopy					619:630	FTIR spectroscopy	614:630	FTIR spectroscopy	614:630	FTIR spectroscopy was indicative for the N-acetylation of chitins (chitosans) as well as for the chitin (chitosan) contribution to fungal polysaccharide preparations.
26116390	3	24	theme	complexes	515:523	arg1	degree					428:433	deacetylation degree	414:433	deacetylation degree (DD) of chitosans	414:451	It was found that the N/C ratio correlates well with deacetylation degree (DD) of chitosans and chitin/glucan ratio R(chit) of fungal chitin – β-D-glucan complexes with the exception of some samples having significant nitrogen and/or carbon admixtures.
26116390	3	24	theme	complexes	515:523	arg1	chit					479:482	chit	479:482	chit	479:482	It was found that the N/C ratio correlates well with deacetylation degree (DD) of chitosans and chitin/glucan ratio R(chit) of fungal chitin – β-D-glucan complexes with the exception of some samples having significant nitrogen and/or carbon admixtures.
26116390	3	24	theme	complexes	515:523	arg1	R					477:477	chitin/glucan ratio R	457:477	chitin/glucan ratio R(chit)	457:483	It was found that the N/C ratio correlates well with deacetylation degree (DD) of chitosans and chitin/glucan ratio R(chit) of fungal chitin – β-D-glucan complexes with the exception of some samples having significant nitrogen and/or carbon admixtures.
26116390	3	24	theme	complexes	515:523	arg1	DD					436:437	DD	436:437	DD	436:437	It was found that the N/C ratio correlates well with deacetylation degree (DD) of chitosans and chitin/glucan ratio R(chit) of fungal chitin – β-D-glucan complexes with the exception of some samples having significant nitrogen and/or carbon admixtures.
26116390	0	25	theme	polysaccharides	22:36	arg1	Distinction					0:10	Distinction	0:10	Distinction of fungal polysaccharides by N/C ratio and mid infrared spectroscopy	0:79	Distinction of fungal polysaccharides by N/C ratio and mid infrared spectroscopy.
26116390	3	26	theme	chitin	495:500	arg1	complexes					515:523	fungal chitin – β-D-glucan complexes	488:523	fungal chitin – β-D-glucan complexes with the exception of some samples having significant nitrogen and/or carbon admixtures	488:611	It was found that the N/C ratio correlates well with deacetylation degree (DD) of chitosans and chitin/glucan ratio R(chit) of fungal chitin – β-D-glucan complexes with the exception of some samples having significant nitrogen and/or carbon admixtures.
26116390	7	27	used	used	1215:1218	arg2	procedures					1132:1141	The proposed procedures	1119:1141	The proposed procedures based on the N/C ratio and multivariate analyses of FTIR spectra	1119:1206	The proposed procedures based on the N/C ratio and multivariate analyses of FTIR spectra may be used in screening fungal polysaccharide preparations.
26116390	5	28	theme	Multivariate	781:792	arg1	analyses					794:801	Multivariate analyses	781:801	Multivariate analyses of the FTIR data (HCA, PCA)	781:829	Multivariate analyses of the FTIR data (HCA, PCA) discriminated samples and reference materials into several clusters depending on their similarity.
26116390	0	29	theme	fungal	15:20	arg1	polysaccharides					22:36	fungal polysaccharides	15:36	fungal polysaccharides	15:36	Distinction of fungal polysaccharides by N/C ratio and mid infrared spectroscopy.
26116390	5	30	theme	several	882:888	arg1	clusters					890:897	several clusters	882:897	several clusters	882:897	Multivariate analyses of the FTIR data (HCA, PCA) discriminated samples and reference materials into several clusters depending on their similarity.
26116390	3	31	theme	carbon	595:600	arg1	admixtures					602:611	significant nitrogen and/or carbon admixtures	567:611	significant nitrogen and/or carbon admixtures	567:611	It was found that the N/C ratio correlates well with deacetylation degree (DD) of chitosans and chitin/glucan ratio R(chit) of fungal chitin – β-D-glucan complexes with the exception of some samples having significant nitrogen and/or carbon admixtures.
26116390	1	32	theme	fungal	91:96	arg1	samples					113:119	fungal polysaccharide samples	91:119	fungal polysaccharide samples	91:119	A set of fungal polysaccharide samples was characterised by elemental analysis and FTIR spectroscopy and compared with reference chitins, chitosans and β-D-glucans.
26116390	1	33	theme	reference	201:209	arg1	chitins					211:217	reference chitins	201:217	reference chitins	201:217	A set of fungal polysaccharide samples was characterised by elemental analysis and FTIR spectroscopy and compared with reference chitins, chitosans and β-D-glucans.
26116390	0	34	theme	N/C	41:43	arg1	ratio					45:49	N/C ratio	41:49	N/C ratio	41:49	Distinction of fungal polysaccharides by N/C ratio and mid infrared spectroscopy.
26116390	6	35	theme	reference	1072:1080	arg1	polysaccharides					1082:1096	the reference polysaccharides	1068:1096	the reference polysaccharides	1068:1096	Chitosan lactates, chitosan – β-D-glucans and chitin – β-D-glucans of high and low amounts of chitin were successfully discriminated from the reference polysaccharides and from each other.
26116390	7	36	theme	fungal	1233:1238	arg1	preparations					1255:1266	screening fungal polysaccharide preparations	1223:1266	screening fungal polysaccharide preparations	1223:1266	The proposed procedures based on the N/C ratio and multivariate analyses of FTIR spectra may be used in screening fungal polysaccharide preparations.
26116390	4	37	theme	polysaccharide	752:765	arg1	preparations					767:778	fungal polysaccharide preparations	745:778	fungal polysaccharide preparations	745:778	FTIR spectroscopy was indicative for the N-acetylation of chitins (chitosans) as well as for the chitin (chitosan) contribution to fungal polysaccharide preparations.
26116390	3	38	theme	deacetylation	414:426	arg1	degree					428:433	deacetylation degree	414:433	deacetylation degree (DD) of chitosans	414:451	It was found that the N/C ratio correlates well with deacetylation degree (DD) of chitosans and chitin/glucan ratio R(chit) of fungal chitin – β-D-glucan complexes with the exception of some samples having significant nitrogen and/or carbon admixtures.
26116390	3	38	theme	deacetylation	414:426	arg1	DD					436:437	DD	436:437	DD	436:437	It was found that the N/C ratio correlates well with deacetylation degree (DD) of chitosans and chitin/glucan ratio R(chit) of fungal chitin – β-D-glucan complexes with the exception of some samples having significant nitrogen and/or carbon admixtures.
26116390	3	39	theme	N/C	383:385	arg1	ratio					387:391	the N/C ratio	379:391	the N/C ratio	379:391	It was found that the N/C ratio correlates well with deacetylation degree (DD) of chitosans and chitin/glucan ratio R(chit) of fungal chitin – β-D-glucan complexes with the exception of some samples having significant nitrogen and/or carbon admixtures.
26116390	3	40	theme	nitrogen	579:586	arg1	admixtures					602:611	significant nitrogen and/or carbon admixtures	567:611	significant nitrogen and/or carbon admixtures	567:611	It was found that the N/C ratio correlates well with deacetylation degree (DD) of chitosans and chitin/glucan ratio R(chit) of fungal chitin – β-D-glucan complexes with the exception of some samples having significant nitrogen and/or carbon admixtures.
26116390	4	41	theme	fungal	745:750	arg1	preparations					767:778	fungal polysaccharide preparations	745:778	fungal polysaccharide preparations	745:778	FTIR spectroscopy was indicative for the N-acetylation of chitins (chitosans) as well as for the chitin (chitosan) contribution to fungal polysaccharide preparations.
26116390	6	42	theme	–	983:983	arg1	β-D-glucans					985:995	chitin – β-D-glucans	976:995	chitin – β-D-glucans	976:995	Chitosan lactates, chitosan – β-D-glucans and chitin – β-D-glucans of high and low amounts of chitin were successfully discriminated from the reference polysaccharides and from each other.
26116390	1	43	theme	polysaccharide	98:111	arg1	samples					113:119	fungal polysaccharide samples	91:119	fungal polysaccharide samples	91:119	A set of fungal polysaccharide samples was characterised by elemental analysis and FTIR spectroscopy and compared with reference chitins, chitosans and β-D-glucans.
26116390	5	44	dep	data	815:818	arg1	PCA					826:828	PCA	826:828	PCA	826:828	Multivariate analyses of the FTIR data (HCA, PCA) discriminated samples and reference materials into several clusters depending on their similarity.
26116390	5	44	dep	data	815:818	arg1	HCA					821:823	HCA	821:823	HCA	821:823	Multivariate analyses of the FTIR data (HCA, PCA) discriminated samples and reference materials into several clusters depending on their similarity.
26116390	1	45	theme	samples	113:119	arg1	set					84:86	A set	82:86	A set of fungal polysaccharide samples	82:119	A set of fungal polysaccharide samples was characterised by elemental analysis and FTIR spectroscopy and compared with reference chitins, chitosans and β-D-glucans.
26116390	1	45	theme	samples	113:119	arg1	samples					113:119	fungal polysaccharide samples	91:119	fungal polysaccharide samples	91:119	A set of fungal polysaccharide samples was characterised by elemental analysis and FTIR spectroscopy and compared with reference chitins, chitosans and β-D-glucans.
26116390	4	46	theme	chitins	672:678	arg1	N-acetylation					655:667	the N-acetylation	651:667	the N-acetylation of chitins (chitosans)	651:690	FTIR spectroscopy was indicative for the N-acetylation of chitins (chitosans) as well as for the chitin (chitosan) contribution to fungal polysaccharide preparations.
26116390	2	47	theme	carbon	263:268	arg1	values					276:281	The nitrogen to carbon (N/C) values	247:281	The nitrogen to carbon (N/C) values	247:281	The nitrogen to carbon (N/C) values and FTIR spectra were used to compare the samples based on their composition.
26116390	5	48	theme	reference	857:865	arg1	materials					867:875	reference materials	857:875	reference materials	857:875	Multivariate analyses of the FTIR data (HCA, PCA) discriminated samples and reference materials into several clusters depending on their similarity.
26116390	2	49	theme	FTIR	287:290	arg1	spectra					292:298	FTIR spectra	287:298	FTIR spectra	287:298	The nitrogen to carbon (N/C) values and FTIR spectra were used to compare the samples based on their composition.
26116390	6	50	theme	chitin	976:981	arg1	β-D-glucans					985:995	chitin – β-D-glucans	976:995	chitin – β-D-glucans	976:995	Chitosan lactates, chitosan – β-D-glucans and chitin – β-D-glucans of high and low amounts of chitin were successfully discriminated from the reference polysaccharides and from each other.
26116390	7	51	theme	screening	1223:1231	arg1	preparations					1255:1266	screening fungal polysaccharide preparations	1223:1266	screening fungal polysaccharide preparations	1223:1266	The proposed procedures based on the N/C ratio and multivariate analyses of FTIR spectra may be used in screening fungal polysaccharide preparations.
26116390	3	52	theme	significant	567:577	arg1	nitrogen					579:586	significant nitrogen	567:586	significant nitrogen	567:586	It was found that the N/C ratio correlates well with deacetylation degree (DD) of chitosans and chitin/glucan ratio R(chit) of fungal chitin – β-D-glucan complexes with the exception of some samples having significant nitrogen and/or carbon admixtures.
26116390	0	53	theme	infrared	59:66	arg1	spectroscopy					68:79	mid infrared spectroscopy	55:79	mid infrared spectroscopy	55:79	Distinction of fungal polysaccharides by N/C ratio and mid infrared spectroscopy.
26116390	7	54	theme	polysaccharide	1240:1253	arg1	preparations					1255:1266	screening fungal polysaccharide preparations	1223:1266	screening fungal polysaccharide preparations	1223:1266	The proposed procedures based on the N/C ratio and multivariate analyses of FTIR spectra may be used in screening fungal polysaccharide preparations.
26116390	2	55	theme	nitrogen	251:258	arg1	values					276:281	The nitrogen to carbon (N/C) values	247:281	The nitrogen to carbon (N/C) values	247:281	The nitrogen to carbon (N/C) values and FTIR spectra were used to compare the samples based on their composition.
26116390	6	56	theme	high	1000:1003	arg1	chitin					1024:1029	chitin	1024:1029	chitin	1024:1029	Chitosan lactates, chitosan – β-D-glucans and chitin – β-D-glucans of high and low amounts of chitin were successfully discriminated from the reference polysaccharides and from each other.
26116390	6	56	theme	high	1000:1003	arg1	amounts					1013:1019	high and low amounts	1000:1019	high and low amounts of chitin	1000:1029	Chitosan lactates, chitosan – β-D-glucans and chitin – β-D-glucans of high and low amounts of chitin were successfully discriminated from the reference polysaccharides and from each other.
26116390	7	57	theme	spectra	1200:1206	arg1	ratio					1160:1164	the N/C ratio	1152:1164	the N/C ratio	1152:1164	The proposed procedures based on the N/C ratio and multivariate analyses of FTIR spectra may be used in screening fungal polysaccharide preparations.
26116390	7	57	theme	spectra	1200:1206	arg1	analyses					1183:1190	multivariate analyses	1170:1190	multivariate analyses of FTIR spectra	1170:1206	The proposed procedures based on the N/C ratio and multivariate analyses of FTIR spectra may be used in screening fungal polysaccharide preparations.
26116390	6	58	theme	amounts	1013:1019	arg1	β-D-glucans					960:970	chitosan – β-D-glucans	949:970	chitosan – β-D-glucans	949:970	Chitosan lactates, chitosan – β-D-glucans and chitin – β-D-glucans of high and low amounts of chitin were successfully discriminated from the reference polysaccharides and from each other.
26116390	6	58	theme	amounts	1013:1019	arg1	lactates					939:946	Chitosan lactates	930:946	Chitosan lactates	930:946	Chitosan lactates, chitosan – β-D-glucans and chitin – β-D-glucans of high and low amounts of chitin were successfully discriminated from the reference polysaccharides and from each other.
26116390	6	58	theme	amounts	1013:1019	arg1	β-D-glucans					985:995	chitin – β-D-glucans	976:995	chitin – β-D-glucans	976:995	Chitosan lactates, chitosan – β-D-glucans and chitin – β-D-glucans of high and low amounts of chitin were successfully discriminated from the reference polysaccharides and from each other.
26116390	0	59	theme	mid	55:57	arg1	spectroscopy					68:79	mid infrared spectroscopy	55:79	mid infrared spectroscopy	55:79	Distinction of fungal polysaccharides by N/C ratio and mid infrared spectroscopy.
26116390	3	60	theme	samples	552:558	arg1	exception					534:542	the exception	530:542	the exception of some samples having significant nitrogen and/or carbon admixtures	530:611	It was found that the N/C ratio correlates well with deacetylation degree (DD) of chitosans and chitin/glucan ratio R(chit) of fungal chitin – β-D-glucan complexes with the exception of some samples having significant nitrogen and/or carbon admixtures.
26116390	1	61	theme	elemental	142:150	arg1	analysis					152:159	elemental analysis	142:159	elemental analysis	142:159	A set of fungal polysaccharide samples was characterised by elemental analysis and FTIR spectroscopy and compared with reference chitins, chitosans and β-D-glucans.
26116390	3	62	with	complexes	515:523	arg1	exception					534:542	the exception	530:542	the exception of some samples having significant nitrogen and/or carbon admixtures	530:611	It was found that the N/C ratio correlates well with deacetylation degree (DD) of chitosans and chitin/glucan ratio R(chit) of fungal chitin – β-D-glucan complexes with the exception of some samples having significant nitrogen and/or carbon admixtures.
26116390	3	63	contain	having	560:565	arg1	samples					552:558	some samples	547:558	some samples having significant nitrogen and/or carbon admixtures	547:611	It was found that the N/C ratio correlates well with deacetylation degree (DD) of chitosans and chitin/glucan ratio R(chit) of fungal chitin – β-D-glucan complexes with the exception of some samples having significant nitrogen and/or carbon admixtures.
26116390	3	63	contain	having	560:565	arg2	admixtures					602:611	significant nitrogen and/or carbon admixtures	567:611	significant nitrogen and/or carbon admixtures	567:611	It was found that the N/C ratio correlates well with deacetylation degree (DD) of chitosans and chitin/glucan ratio R(chit) of fungal chitin – β-D-glucan complexes with the exception of some samples having significant nitrogen and/or carbon admixtures.
27166668	0	0	theme	microorganisms	83:96	arg1	activity					58:65	biocontrol activity	47:65	biocontrol activity of antagonistic microorganisms	47:96	Agar composition affects in vitro screening of biocontrol activity of antagonistic microorganisms.
27166668	2	1	theme	antagonist	349:358	arg1	activity					325:332	the activity	321:332	the activity of a bacterial antagonist against Agrobacterium	321:380	We showed that when using the same medium, but different agar compositions, the activity of a bacterial antagonist against Agrobacterium was strongly affected.
27166668	2	2	theme	agar	302:305	arg1	compositions					307:318	different agar compositions	292:318	different agar compositions	292:318	We showed that when using the same medium, but different agar compositions, the activity of a bacterial antagonist against Agrobacterium was strongly affected.
27166668	2	3	theme	same	275:278	arg1	medium					280:285	the same medium	271:285	the same medium	271:285	We showed that when using the same medium, but different agar compositions, the activity of a bacterial antagonist against Agrobacterium was strongly affected.
27166668	0	4	dep	in	25:26	arg1	vitro					28:32	vitro	28:32	vitro	28:32	Agar composition affects in vitro screening of biocontrol activity of antagonistic microorganisms.
27166668	0	5	theme	antagonistic	70:81	arg1	microorganisms					83:96	antagonistic microorganisms	70:96	antagonistic microorganisms	70:96	Agar composition affects in vitro screening of biocontrol activity of antagonistic microorganisms.
27166668	2	6	theme	bacterial	339:347	arg1	antagonist					349:358	a bacterial antagonist	337:358	a bacterial antagonist	337:358	We showed that when using the same medium, but different agar compositions, the activity of a bacterial antagonist against Agrobacterium was strongly affected.
27166668	3	7	dep	in	432:433	arg1	vitro					435:439	vitro	435:439	vitro	435:439	Consequently, results from in vitro screenings should be interpreted cautiously.
27166668	1	8	theme	antagonistic	168:179	arg1	potential					181:189	antagonistic potential	168:189	antagonistic potential of bacterial biocontrol-candidates against pathogens	168:242	Agar-based screening assays are the method of choice when evaluating antagonistic potential of bacterial biocontrol-candidates against pathogens.
27166668	3	9	from	screenings	441:450	arg1	results					419:425	results	419:425	results from in vitro screenings	419:450	Consequently, results from in vitro screenings should be interpreted cautiously.
27166668	1	10	theme	bacterial	194:202	arg1	biocontrol-candidates					204:224	bacterial biocontrol-candidates	194:224	bacterial biocontrol-candidates	194:224	Agar-based screening assays are the method of choice when evaluating antagonistic potential of bacterial biocontrol-candidates against pathogens.
27166668	0	11	theme	Agar	0:3	arg1	composition					5:15	Agar composition	0:15	Agar composition	0:15	Agar composition affects in vitro screening of biocontrol activity of antagonistic microorganisms.
27166668	0	12	theme	biocontrol	47:56	arg1	activity					58:65	biocontrol activity	47:65	biocontrol activity of antagonistic microorganisms	47:96	Agar composition affects in vitro screening of biocontrol activity of antagonistic microorganisms.
27166668	1	13	theme	Agar-based	99:108	arg1	method					135:140	the method	131:140	the method of choice	131:150	Agar-based screening assays are the method of choice when evaluating antagonistic potential of bacterial biocontrol-candidates against pathogens.
27166668	1	13	theme	Agar-based	99:108	arg1	assays					120:125	Agar-based screening assays	99:125	Agar-based screening assays	99:125	Agar-based screening assays are the method of choice when evaluating antagonistic potential of bacterial biocontrol-candidates against pathogens.
27166668	1	14	theme	choice	145:150	arg1	method					135:140	the method	131:140	the method of choice	131:150	Agar-based screening assays are the method of choice when evaluating antagonistic potential of bacterial biocontrol-candidates against pathogens.
27166668	1	14	theme	choice	145:150	arg1	assays					120:125	Agar-based screening assays	99:125	Agar-based screening assays	99:125	Agar-based screening assays are the method of choice when evaluating antagonistic potential of bacterial biocontrol-candidates against pathogens.
27166668	1	15	theme	biocontrol-candidates	204:224	arg1	potential					181:189	antagonistic potential	168:189	antagonistic potential of bacterial biocontrol-candidates against pathogens	168:242	Agar-based screening assays are the method of choice when evaluating antagonistic potential of bacterial biocontrol-candidates against pathogens.
27166668	0	16	theme	in	25:26	arg1	screening					34:42	in vitro screening	25:42	in vitro screening of biocontrol activity of antagonistic microorganisms	25:96	Agar composition affects in vitro screening of biocontrol activity of antagonistic microorganisms.
27166668	0	17	theme	activity	58:65	arg1	screening					34:42	in vitro screening	25:42	in vitro screening of biocontrol activity of antagonistic microorganisms	25:96	Agar composition affects in vitro screening of biocontrol activity of antagonistic microorganisms.
27166668	3	18	theme	in	432:433	arg1	screenings					441:450	in vitro screenings	432:450	in vitro screenings	432:450	Consequently, results from in vitro screenings should be interpreted cautiously.
27166668	1	19	theme	screening	110:118	arg1	method					135:140	the method	131:140	the method of choice	131:150	Agar-based screening assays are the method of choice when evaluating antagonistic potential of bacterial biocontrol-candidates against pathogens.
27166668	1	19	theme	screening	110:118	arg1	assays					120:125	Agar-based screening assays	99:125	Agar-based screening assays	99:125	Agar-based screening assays are the method of choice when evaluating antagonistic potential of bacterial biocontrol-candidates against pathogens.
27166668	2	20	theme	different	292:300	arg1	compositions					307:318	different agar compositions	292:318	different agar compositions	292:318	We showed that when using the same medium, but different agar compositions, the activity of a bacterial antagonist against Agrobacterium was strongly affected.
26428152	0	0	theme	poly	82:85	arg1	crystallization					63:77	crystallization	63:77	crystallization of poly(β-hydroxybutyrate)	63:104	Insights into the nucleation role of cellulose crystals during crystallization of poly(β-hydroxybutyrate).
26428152	2	1	theme	temperature	568:578	arg1	increase					556:563	increase	556:563	increase of temperature	556:578	The results reveal that the spherulite morphology of PHB and its composites depends highly on the crystallization temperature, evolving from bundle shaped to ring-banded and finally to irregular or zigzag textures with increase of temperature.
26428152	5	2	theme	kinetic	1179:1185	arg1	analyses					1187:1194	the kinetic analyses	1175:1194	the kinetic analyses	1175:1194	Besides, cellulose crystals can act as good heterogeneous nucleating agent to accelerate the crystallization of PHB, which is further confirmed by the polarized optical microscopy observations and the kinetic analyses.
26428152	2	3	theme	crystallization	435:449	arg1	temperature					451:461	the crystallization temperature	431:461	the crystallization temperature	431:461	The results reveal that the spherulite morphology of PHB and its composites depends highly on the crystallization temperature, evolving from bundle shaped to ring-banded and finally to irregular or zigzag textures with increase of temperature.
26428152	1	4	theme	melt	298:301	arg1	mixing					303:308	melt mixing	298:308	melt mixing for crystallization study	298:334	Cellulose crystals, including microcrystalline cellulose (MCC) and nanocrystalline cellulose (NCC), were used as the fillers to prepare green composites with poly(β-hydroxybutyrate) (PHB) by melt mixing for crystallization study.
26428152	4	5	theme	spherulite	822:831	arg1	structure					833:841	degraded spherulite structure	813:841	degraded spherulite structure with smaller band space	813:865	Compared with PHB/MCC composite, PHB/NCC composite shows degraded spherulite structure with smaller band space and higher flocculation level of peak-to-valley height because of stronger unbalanced stresses in this system.
26428152	2	6	with	textures	542:549	arg1	increase					556:563	increase	556:563	increase of temperature	556:578	The results reveal that the spherulite morphology of PHB and its composites depends highly on the crystallization temperature, evolving from bundle shaped to ring-banded and finally to irregular or zigzag textures with increase of temperature.
26428152	3	7	theme	cellulose	656:664	arg1	crystals					666:673	cellulose crystals	656:673	cellulose crystals	656:673	However, the ring-banded structure is strongly affected by the presence of cellulose crystals, and the average band space decreases evidently with the addition of MCC or NCC.
26428152	4	8	theme	smaller	848:854	arg1	space					861:865	smaller band space	848:865	smaller band space	848:865	Compared with PHB/MCC composite, PHB/NCC composite shows degraded spherulite structure with smaller band space and higher flocculation level of peak-to-valley height because of stronger unbalanced stresses in this system.
26428152	4	9	theme	height	915:920	arg1	structure					833:841	degraded spherulite structure	813:841	degraded spherulite structure with smaller band space	813:865	Compared with PHB/MCC composite, PHB/NCC composite shows degraded spherulite structure with smaller band space and higher flocculation level of peak-to-valley height because of stronger unbalanced stresses in this system.
26428152	4	9	theme	height	915:920	arg1	level					891:895	higher flocculation level	871:895	higher flocculation level of peak-to-valley height	871:920	Compared with PHB/MCC composite, PHB/NCC composite shows degraded spherulite structure with smaller band space and higher flocculation level of peak-to-valley height because of stronger unbalanced stresses in this system.
26428152	3	10	theme	average	684:690	arg1	space					697:701	the average band space	680:701	the average band space	680:701	However, the ring-banded structure is strongly affected by the presence of cellulose crystals, and the average band space decreases evidently with the addition of MCC or NCC.
26428152	1	11	theme	green	243:247	arg1	composites					249:258	green composites	243:258	green composites	243:258	Cellulose crystals, including microcrystalline cellulose (MCC) and nanocrystalline cellulose (NCC), were used as the fillers to prepare green composites with poly(β-hydroxybutyrate) (PHB) by melt mixing for crystallization study.
26428152	4	12	theme	stronger	933:940	arg1	stresses					953:960	stronger unbalanced stresses	933:960	stronger unbalanced stresses in this system	933:975	Compared with PHB/MCC composite, PHB/NCC composite shows degraded spherulite structure with smaller band space and higher flocculation level of peak-to-valley height because of stronger unbalanced stresses in this system.
26428152	3	13	theme	band	692:695	arg1	space					697:701	the average band space	680:701	the average band space	680:701	However, the ring-banded structure is strongly affected by the presence of cellulose crystals, and the average band space decreases evidently with the addition of MCC or NCC.
26428152	5	14	dep	good	1017:1020	arg1	heterogeneous					1022:1034	heterogeneous	1022:1034	heterogeneous	1022:1034	Besides, cellulose crystals can act as good heterogeneous nucleating agent to accelerate the crystallization of PHB, which is further confirmed by the polarized optical microscopy observations and the kinetic analyses.
26428152	5	15	theme	PHB	1090:1092	arg1	crystallization					1071:1085	the crystallization	1067:1085	the crystallization	1067:1085	Besides, cellulose crystals can act as good heterogeneous nucleating agent to accelerate the crystallization of PHB, which is further confirmed by the polarized optical microscopy observations and the kinetic analyses.
26428152	4	16	theme	PHB/NCC	789:795	arg1	composite					797:805	PHB/NCC composite	789:805	PHB/NCC composite	789:805	Compared with PHB/MCC composite, PHB/NCC composite shows degraded spherulite structure with smaller band space and higher flocculation level of peak-to-valley height because of stronger unbalanced stresses in this system.
26428152	4	17	with	level	891:895	arg1	space					861:865	smaller band space	848:865	smaller band space	848:865	Compared with PHB/MCC composite, PHB/NCC composite shows degraded spherulite structure with smaller band space and higher flocculation level of peak-to-valley height because of stronger unbalanced stresses in this system.
26428152	2	18	theme	composites	402:411	arg1	morphology					376:385	the spherulite morphology	361:385	the spherulite morphology of PHB and its composites	361:411	The results reveal that the spherulite morphology of PHB and its composites depends highly on the crystallization temperature, evolving from bundle shaped to ring-banded and finally to irregular or zigzag textures with increase of temperature.
26428152	4	19	theme	degraded	813:820	arg1	structure					833:841	degraded spherulite structure	813:841	degraded spherulite structure with smaller band space	813:865	Compared with PHB/MCC composite, PHB/NCC composite shows degraded spherulite structure with smaller band space and higher flocculation level of peak-to-valley height because of stronger unbalanced stresses in this system.
26428152	1	20	theme	crystallization	314:328	arg1	study					330:334	crystallization study	314:334	crystallization study	314:334	Cellulose crystals, including microcrystalline cellulose (MCC) and nanocrystalline cellulose (NCC), were used as the fillers to prepare green composites with poly(β-hydroxybutyrate) (PHB) by melt mixing for crystallization study.
26428152	1	21	theme	nanocrystalline	174:188	arg1	NCC					201:203	NCC	201:203	NCC	201:203	Cellulose crystals, including microcrystalline cellulose (MCC) and nanocrystalline cellulose (NCC), were used as the fillers to prepare green composites with poly(β-hydroxybutyrate) (PHB) by melt mixing for crystallization study.
26428152	1	21	theme	nanocrystalline	174:188	arg1	cellulose					190:198	nanocrystalline cellulose	174:198	nanocrystalline cellulose (NCC)	174:204	Cellulose crystals, including microcrystalline cellulose (MCC) and nanocrystalline cellulose (NCC), were used as the fillers to prepare green composites with poly(β-hydroxybutyrate) (PHB) by melt mixing for crystallization study.
26428152	5	22	dep	microscopy	1147:1156	arg1	observations					1158:1169	observations	1158:1169	the polarized optical microscopy observations	1125:1169	Besides, cellulose crystals can act as good heterogeneous nucleating agent to accelerate the crystallization of PHB, which is further confirmed by the polarized optical microscopy observations and the kinetic analyses.
26428152	0	23	theme	nucleation	18:27	arg1	role					29:32	the nucleation role	14:32	the nucleation role of cellulose crystals during crystallization of poly(β-hydroxybutyrate)	14:104	Insights into the nucleation role of cellulose crystals during crystallization of poly(β-hydroxybutyrate).
26428152	5	24	theme	cellulose	987:995	arg1	crystals					997:1004	cellulose crystals	987:1004	cellulose crystals	987:1004	Besides, cellulose crystals can act as good heterogeneous nucleating agent to accelerate the crystallization of PHB, which is further confirmed by the polarized optical microscopy observations and the kinetic analyses.
26428152	2	25	theme	PHB	390:392	arg1	morphology					376:385	the spherulite morphology	361:385	the spherulite morphology of PHB and its composites	361:411	The results reveal that the spherulite morphology of PHB and its composites depends highly on the crystallization temperature, evolving from bundle shaped to ring-banded and finally to irregular or zigzag textures with increase of temperature.
26428152	4	26	theme	flocculation	878:889	arg1	level					891:895	higher flocculation level	871:895	higher flocculation level of peak-to-valley height	871:920	Compared with PHB/MCC composite, PHB/NCC composite shows degraded spherulite structure with smaller band space and higher flocculation level of peak-to-valley height because of stronger unbalanced stresses in this system.
26428152	4	27	theme	peak-to-valley	900:913	arg1	height					915:920	peak-to-valley height	900:920	peak-to-valley height	900:920	Compared with PHB/MCC composite, PHB/NCC composite shows degraded spherulite structure with smaller band space and higher flocculation level of peak-to-valley height because of stronger unbalanced stresses in this system.
26428152	4	28	theme	PHB/MCC	770:776	arg1	composite					778:786	PHB/MCC composite	770:786	PHB/MCC composite	770:786	Compared with PHB/MCC composite, PHB/NCC composite shows degraded spherulite structure with smaller band space and higher flocculation level of peak-to-valley height because of stronger unbalanced stresses in this system.
26428152	2	29	theme	spherulite	365:374	arg1	morphology					376:385	the spherulite morphology	361:385	the spherulite morphology of PHB and its composites	361:411	The results reveal that the spherulite morphology of PHB and its composites depends highly on the crystallization temperature, evolving from bundle shaped to ring-banded and finally to irregular or zigzag textures with increase of temperature.
26428152	3	30	theme	ring-banded	594:604	arg1	structure					606:614	the ring-banded structure	590:614	the ring-banded structure	590:614	However, the ring-banded structure is strongly affected by the presence of cellulose crystals, and the average band space decreases evidently with the addition of MCC or NCC.
26428152	5	31	theme	optical	1139:1145	arg1	microscopy					1147:1156	the polarized optical microscopy observations	1125:1169	the polarized optical microscopy observations	1125:1169	Besides, cellulose crystals can act as good heterogeneous nucleating agent to accelerate the crystallization of PHB, which is further confirmed by the polarized optical microscopy observations and the kinetic analyses.
26428152	0	32	theme	crystals	47:54	arg1	role					29:32	the nucleation role	14:32	the nucleation role of cellulose crystals during crystallization of poly(β-hydroxybutyrate)	14:104	Insights into the nucleation role of cellulose crystals during crystallization of poly(β-hydroxybutyrate).
26428152	3	33	theme	MCC	744:746	arg1	addition					732:739	the addition	728:739	the addition of MCC or NCC	728:753	However, the ring-banded structure is strongly affected by the presence of cellulose crystals, and the average band space decreases evidently with the addition of MCC or NCC.
26428152	1	34	theme	Cellulose	107:115	arg1	cellulose					154:162	microcrystalline cellulose	137:162	microcrystalline cellulose (MCC)	137:168	Cellulose crystals, including microcrystalline cellulose (MCC) and nanocrystalline cellulose (NCC), were used as the fillers to prepare green composites with poly(β-hydroxybutyrate) (PHB) by melt mixing for crystallization study.
26428152	1	34	theme	Cellulose	107:115	arg1	cellulose					190:198	nanocrystalline cellulose	174:198	nanocrystalline cellulose (NCC)	174:204	Cellulose crystals, including microcrystalline cellulose (MCC) and nanocrystalline cellulose (NCC), were used as the fillers to prepare green composites with poly(β-hydroxybutyrate) (PHB) by melt mixing for crystallization study.
26428152	1	34	theme	Cellulose	107:115	arg1	crystals					117:124	Cellulose crystals	107:124	Cellulose crystals	107:124	Cellulose crystals, including microcrystalline cellulose (MCC) and nanocrystalline cellulose (NCC), were used as the fillers to prepare green composites with poly(β-hydroxybutyrate) (PHB) by melt mixing for crystallization study.
26428152	1	34	theme	Cellulose	107:115	arg1	fillers					224:230	the fillers	220:230	the fillers	220:230	Cellulose crystals, including microcrystalline cellulose (MCC) and nanocrystalline cellulose (NCC), were used as the fillers to prepare green composites with poly(β-hydroxybutyrate) (PHB) by melt mixing for crystallization study.
26428152	0	35	theme	cellulose	37:45	arg1	crystals					47:54	cellulose crystals	37:54	cellulose crystals	37:54	Insights into the nucleation role of cellulose crystals during crystallization of poly(β-hydroxybutyrate).
26428152	5	36	theme	polarized	1129:1137	arg1	microscopy					1147:1156	the polarized optical microscopy observations	1125:1169	the polarized optical microscopy observations	1125:1169	Besides, cellulose crystals can act as good heterogeneous nucleating agent to accelerate the crystallization of PHB, which is further confirmed by the polarized optical microscopy observations and the kinetic analyses.
26428152	2	37	theme	zigzag	535:540	arg1	textures					542:549	irregular or zigzag textures	522:549	textures	542:549	The results reveal that the spherulite morphology of PHB and its composites depends highly on the crystallization temperature, evolving from bundle shaped to ring-banded and finally to irregular or zigzag textures with increase of temperature.
26428152	4	38	theme	band	856:859	arg1	space					861:865	smaller band space	848:865	smaller band space	848:865	Compared with PHB/MCC composite, PHB/NCC composite shows degraded spherulite structure with smaller band space and higher flocculation level of peak-to-valley height because of stronger unbalanced stresses in this system.
26428152	4	39	theme	higher	871:876	arg1	level					891:895	higher flocculation level	871:895	higher flocculation level of peak-to-valley height	871:920	Compared with PHB/MCC composite, PHB/NCC composite shows degraded spherulite structure with smaller band space and higher flocculation level of peak-to-valley height because of stronger unbalanced stresses in this system.
26428152	5	40	theme	good	1017:1020	arg1	agent					1047:1051	good heterogeneous nucleating agent	1017:1051	good heterogeneous nucleating agent	1017:1051	Besides, cellulose crystals can act as good heterogeneous nucleating agent to accelerate the crystallization of PHB, which is further confirmed by the polarized optical microscopy observations and the kinetic analyses.
26428152	2	41	theme	irregular	522:530	arg1	textures					542:549	irregular or zigzag textures	522:549	textures	542:549	The results reveal that the spherulite morphology of PHB and its composites depends highly on the crystallization temperature, evolving from bundle shaped to ring-banded and finally to irregular or zigzag textures with increase of temperature.
26428152	1	42	used	used	212:215	arg2	cellulose					154:162	microcrystalline cellulose	137:162	microcrystalline cellulose (MCC)	137:168	Cellulose crystals, including microcrystalline cellulose (MCC) and nanocrystalline cellulose (NCC), were used as the fillers to prepare green composites with poly(β-hydroxybutyrate) (PHB) by melt mixing for crystallization study.
26428152	1	42	used	used	212:215	arg2	cellulose					190:198	nanocrystalline cellulose	174:198	nanocrystalline cellulose (NCC)	174:204	Cellulose crystals, including microcrystalline cellulose (MCC) and nanocrystalline cellulose (NCC), were used as the fillers to prepare green composites with poly(β-hydroxybutyrate) (PHB) by melt mixing for crystallization study.
26428152	1	42	used	used	212:215	arg2	fillers					224:230	the fillers	220:230	the fillers	220:230	Cellulose crystals, including microcrystalline cellulose (MCC) and nanocrystalline cellulose (NCC), were used as the fillers to prepare green composites with poly(β-hydroxybutyrate) (PHB) by melt mixing for crystallization study.
26428152	1	42	used	used	212:215	arg2	crystals					117:124	Cellulose crystals	107:124	Cellulose crystals	107:124	Cellulose crystals, including microcrystalline cellulose (MCC) and nanocrystalline cellulose (NCC), were used as the fillers to prepare green composites with poly(β-hydroxybutyrate) (PHB) by melt mixing for crystallization study.
26428152	4	43	with	structure	833:841	arg1	space					861:865	smaller band space	848:865	smaller band space	848:865	Compared with PHB/MCC composite, PHB/NCC composite shows degraded spherulite structure with smaller band space and higher flocculation level of peak-to-valley height because of stronger unbalanced stresses in this system.
26428152	5	44	theme	nucleating	1036:1045	arg1	agent					1047:1051	good heterogeneous nucleating agent	1017:1051	good heterogeneous nucleating agent	1017:1051	Besides, cellulose crystals can act as good heterogeneous nucleating agent to accelerate the crystallization of PHB, which is further confirmed by the polarized optical microscopy observations and the kinetic analyses.
26428152	1	45	theme	microcrystalline	137:152	arg1	cellulose					154:162	microcrystalline cellulose	137:162	microcrystalline cellulose (MCC)	137:168	Cellulose crystals, including microcrystalline cellulose (MCC) and nanocrystalline cellulose (NCC), were used as the fillers to prepare green composites with poly(β-hydroxybutyrate) (PHB) by melt mixing for crystallization study.
26428152	1	45	theme	microcrystalline	137:152	arg1	MCC					165:167	MCC	165:167	MCC	165:167	Cellulose crystals, including microcrystalline cellulose (MCC) and nanocrystalline cellulose (NCC), were used as the fillers to prepare green composites with poly(β-hydroxybutyrate) (PHB) by melt mixing for crystallization study.
26428152	4	46	from	stresses	953:960	arg1	system					970:975	this system	965:975	this system	965:975	Compared with PHB/MCC composite, PHB/NCC composite shows degraded spherulite structure with smaller band space and higher flocculation level of peak-to-valley height because of stronger unbalanced stresses in this system.
26428152	4	47	theme	unbalanced	942:951	arg1	stresses					953:960	stronger unbalanced stresses	933:960	stronger unbalanced stresses in this system	933:975	Compared with PHB/MCC composite, PHB/NCC composite shows degraded spherulite structure with smaller band space and higher flocculation level of peak-to-valley height because of stronger unbalanced stresses in this system.
26428152	3	48	theme	crystals	666:673	arg1	presence					644:651	the presence	640:651	the presence of cellulose crystals	640:673	However, the ring-banded structure is strongly affected by the presence of cellulose crystals, and the average band space decreases evidently with the addition of MCC or NCC.
26428152	3	49	theme	NCC	751:753	arg1	addition					732:739	the addition	728:739	the addition of MCC or NCC	728:753	However, the ring-banded structure is strongly affected by the presence of cellulose crystals, and the average band space decreases evidently with the addition of MCC or NCC.
27312636	9	0	theme	GTP	1556:1558	arg1	conditions					1560:1569	severe GTP conditions	1549:1569	severe GTP conditions	1549:1569	Furthermore, crude WSX contained less xylan with more lignin contamination at severe GTP conditions.
27312636	4	1	theme	precipitated	676:687	arg1	portion					774:780	a water insoluble xylan portion	750:780	a water insoluble xylan portion (WIX)	750:786	The precipitated xylan from the neutralized alkaline filtrate was isolated as a water insoluble xylan portion (WIX).
27312636	4	1	theme	precipitated	676:687	arg1	xylan					689:693	The precipitated xylan	672:693	The precipitated xylan from the neutralized alkaline filtrate	672:732	The precipitated xylan from the neutralized alkaline filtrate was isolated as a water insoluble xylan portion (WIX).
27312636	1	2	theme	biopolymer	169:178	arg1	fractionation					180:192	stepwise biopolymer fractionation	160:192	stepwise biopolymer fractionation	160:192	Glycerol thermal processing (GTP) of hardwood biomass at temperatures between 200 and 240°C facilitated stepwise biopolymer fractionation, while limiting significant degradation of the major hemicellulose, glucuronoxylan, into water-extractable oligosaccharides.
27312636	6	3	theme	27	1102:1103	arg1	DP					1096:1097	a much lower average DP	1075:1097	a much lower average DP of 27 (ca)	1075:1108	Results showed that xylan in WIX was in a polymeric form with a number average degree of polymerization (DP) over 100, whereas the WSX had a much lower average DP of 27 (ca) and contained more substitution.
27312636	10	4	theme	narrow	1698:1703	arg1	index					1720:1724	an extremely narrow polydispersity index	1685:1724	an extremely narrow polydispersity index between 1.1 and 1.25	1685:1745	The recovery of the xylan in two portions facilitated a preferential purification strategy resulting in WIX with an extremely narrow polydispersity index between 1.1 and 1.25, dependent upon the GTP severity.
27312636	2	5	theme	GTP	325:327	arg1	pretreatment					329:340	GTP pretreatment	325:340	GTP pretreatment	325:340	After GTP pretreatment and sequential water and organic solvent extraction, up to 80% of the initial xylan remained in the pretreated biomass.
27312636	5	6	theme	cold	866:869	arg1	methanol					871:878	cold methanol	866:878	cold methanol	866:878	The residual xylan dissolved in the neutralized filtrate was precipitated in cold methanol and recovered as the water soluble xylan portion (WSX).
27312636	11	7	theme	value-added	1873:1883	arg1	applications					1885:1896	value-added applications	1873:1896	value-added applications	1873:1896	This study provided insight into fractionating higher molecular weight xylan that may serve value-added applications such as healthcare materials and advanced packaging.
27312636	3	8	attach	isolated	638:645	arg1	hardwood					662:669	untreated hardwood	652:669	untreated hardwood	652:669	The majority of the xylan from GTP pretreated and water/solvent extracted biomass was removed using a mild alkali extraction and the composition was compared to xylan directly isolated from untreated hardwood.
27312636	3	8	attach	isolated	638:645	arg2	xylan					623:627	xylan	623:627	xylan directly isolated from untreated hardwood	623:669	The majority of the xylan from GTP pretreated and water/solvent extracted biomass was removed using a mild alkali extraction and the composition was compared to xylan directly isolated from untreated hardwood.
27312636	7	9	theme	WIX	1276:1278	arg1	sample					1280:1285	the WIX sample	1272:1285	the WIX sample	1272:1285	As the processing severity increased during GTP pretreatment, the proportion of WIX increased and the purity of the xylan within the WIX sample reached 84% based on compositional analysis.
27312636	6	10	theme	number	1000:1005	arg1	degree					1015:1020	a number average degree	998:1020	a number average degree of polymerization (DP)	998:1043	Results showed that xylan in WIX was in a polymeric form with a number average degree of polymerization (DP) over 100, whereas the WSX had a much lower average DP of 27 (ca) and contained more substitution.
27312636	6	11	with	form	988:991	arg1	degree					1015:1020	a number average degree	998:1020	a number average degree of polymerization (DP)	998:1043	Results showed that xylan in WIX was in a polymeric form with a number average degree of polymerization (DP) over 100, whereas the WSX had a much lower average DP of 27 (ca) and contained more substitution.
27312636	4	12	theme	neutralized	704:714	arg1	filtrate					725:732	the neutralized alkaline filtrate	700:732	the neutralized alkaline filtrate	700:732	The precipitated xylan from the neutralized alkaline filtrate was isolated as a water insoluble xylan portion (WIX).
27312636	11	13	theme	healthcare	1906:1915	arg1	materials					1917:1925	healthcare materials	1906:1925	healthcare materials	1906:1925	This study provided insight into fractionating higher molecular weight xylan that may serve value-added applications such as healthcare materials and advanced packaging.
27312636	5	14	from	methanol	871:878	arg1	precipitated					850:861	precipitated	850:861	precipitated	850:861	The residual xylan dissolved in the neutralized filtrate was precipitated in cold methanol and recovered as the water soluble xylan portion (WSX).
27312636	8	15	theme	related	1409:1415	arg1	peaks					1403:1407	peaks	1403:1407	peaks related to a reduced carbonyl signal compared to the control	1403:1468	FT-IR analysis of WIX revealed that xylan isolated after GTP contained peaks related to a reduced carbonyl signal compared to the control.
27312636	5	16	theme	xylan	915:919	arg1	xylan					802:806	The residual xylan	789:806	The residual xylan dissolved in the neutralized filtrate	789:844	The residual xylan dissolved in the neutralized filtrate was precipitated in cold methanol and recovered as the water soluble xylan portion (WSX).
27312636	5	16	theme	xylan	915:919	arg1	WSX					930:932	WSX	930:932	WSX	930:932	The residual xylan dissolved in the neutralized filtrate was precipitated in cold methanol and recovered as the water soluble xylan portion (WSX).
27312636	5	16	theme	xylan	915:919	arg1	portion					921:927	the water soluble xylan portion	897:927	the water soluble xylan portion (WSX)	897:933	The residual xylan dissolved in the neutralized filtrate was precipitated in cold methanol and recovered as the water soluble xylan portion (WSX).
27312636	6	17	theme	polymeric	978:986	arg1	form					988:991	a polymeric form	976:991	a polymeric form	976:991	Results showed that xylan in WIX was in a polymeric form with a number average degree of polymerization (DP) over 100, whereas the WSX had a much lower average DP of 27 (ca) and contained more substitution.
27312636	1	18	theme	thermal	65:71	arg1	processing					73:82	Glycerol thermal processing	56:82	Glycerol thermal processing (GTP) of hardwood biomass at temperatures between 200 and 240°C	56:146	Glycerol thermal processing (GTP) of hardwood biomass at temperatures between 200 and 240°C facilitated stepwise biopolymer fractionation, while limiting significant degradation of the major hemicellulose, glucuronoxylan, into water-extractable oligosaccharides.
27312636	1	18	theme	thermal	65:71	arg1	GTP					85:87	GTP	85:87	GTP	85:87	Glycerol thermal processing (GTP) of hardwood biomass at temperatures between 200 and 240°C facilitated stepwise biopolymer fractionation, while limiting significant degradation of the major hemicellulose, glucuronoxylan, into water-extractable oligosaccharides.
27312636	8	19	theme	carbonyl	1430:1437	arg1	signal					1439:1444	a reduced carbonyl signal	1420:1444	a reduced carbonyl signal compared to the control	1420:1468	FT-IR analysis of WIX revealed that xylan isolated after GTP contained peaks related to a reduced carbonyl signal compared to the control.
27312636	11	20	theme	higher	1828:1833	arg1	weight					1845:1850	higher molecular weight	1828:1850	higher molecular weight xylan that may serve value-added applications such as healthcare materials and advanced packaging	1828:1948	This study provided insight into fractionating higher molecular weight xylan that may serve value-added applications such as healthcare materials and advanced packaging.
27312636	1	21	from	temperatures	113:124	arg1	processing					73:82	Glycerol thermal processing	56:82	Glycerol thermal processing (GTP) of hardwood biomass at temperatures between 200 and 240°C	56:146	Glycerol thermal processing (GTP) of hardwood biomass at temperatures between 200 and 240°C facilitated stepwise biopolymer fractionation, while limiting significant degradation of the major hemicellulose, glucuronoxylan, into water-extractable oligosaccharides.
27312636	1	21	from	temperatures	113:124	arg1	GTP					85:87	GTP	85:87	GTP	85:87	Glycerol thermal processing (GTP) of hardwood biomass at temperatures between 200 and 240°C facilitated stepwise biopolymer fractionation, while limiting significant degradation of the major hemicellulose, glucuronoxylan, into water-extractable oligosaccharides.
27312636	3	22	dep	pretreated	497:506	arg1	biomass					536:542	extracted biomass	526:542	extracted biomass	526:542	The majority of the xylan from GTP pretreated and water/solvent extracted biomass was removed using a mild alkali extraction and the composition was compared to xylan directly isolated from untreated hardwood.
27312636	7	23	theme	xylan	1259:1263	arg1	purity					1245:1250	the purity	1241:1250	the purity of the xylan within the WIX sample	1241:1285	As the processing severity increased during GTP pretreatment, the proportion of WIX increased and the purity of the xylan within the WIX sample reached 84% based on compositional analysis.
27312636	4	24	theme	insoluble	758:766	arg1	WIX					783:785	WIX	783:785	WIX	783:785	The precipitated xylan from the neutralized alkaline filtrate was isolated as a water insoluble xylan portion (WIX).
27312636	4	24	theme	insoluble	758:766	arg1	portion					774:780	a water insoluble xylan portion	750:780	a water insoluble xylan portion (WIX)	750:786	The precipitated xylan from the neutralized alkaline filtrate was isolated as a water insoluble xylan portion (WIX).
27312636	4	24	theme	insoluble	758:766	arg1	xylan					689:693	The precipitated xylan	672:693	The precipitated xylan from the neutralized alkaline filtrate	672:732	The precipitated xylan from the neutralized alkaline filtrate was isolated as a water insoluble xylan portion (WIX).
27312636	1	25	theme	water-extractable	283:299	arg1	oligosaccharides					301:316	water-extractable oligosaccharides	283:316	water-extractable oligosaccharides	283:316	Glycerol thermal processing (GTP) of hardwood biomass at temperatures between 200 and 240°C facilitated stepwise biopolymer fractionation, while limiting significant degradation of the major hemicellulose, glucuronoxylan, into water-extractable oligosaccharides.
27312636	9	26	theme	crude	1484:1488	arg1	WSX					1490:1492	crude WSX	1484:1492	crude WSX	1484:1492	Furthermore, crude WSX contained less xylan with more lignin contamination at severe GTP conditions.
27312636	2	27	theme	pretreated	442:451	arg1	biomass					453:459	the pretreated biomass	438:459	the pretreated biomass	438:459	After GTP pretreatment and sequential water and organic solvent extraction, up to 80% of the initial xylan remained in the pretreated biomass.
27312636	10	28	theme	xylan	1592:1596	arg1	recovery					1576:1583	The recovery	1572:1583	The recovery of the xylan in two portions	1572:1612	The recovery of the xylan in two portions facilitated a preferential purification strategy resulting in WIX with an extremely narrow polydispersity index between 1.1 and 1.25, dependent upon the GTP severity.
27312636	1	29	theme	hardwood	93:100	arg1	biomass					102:108	hardwood biomass	93:108	hardwood biomass	93:108	Glycerol thermal processing (GTP) of hardwood biomass at temperatures between 200 and 240°C facilitated stepwise biopolymer fractionation, while limiting significant degradation of the major hemicellulose, glucuronoxylan, into water-extractable oligosaccharides.
27312636	6	30	theme	polymerization	1025:1038	arg1	degree					1015:1020	a number average degree	998:1020	a number average degree of polymerization (DP)	998:1043	Results showed that xylan in WIX was in a polymeric form with a number average degree of polymerization (DP) over 100, whereas the WSX had a much lower average DP of 27 (ca) and contained more substitution.
27312636	2	31	theme	xylan	420:424	arg1	xylan					420:424	the initial xylan	408:424	the initial xylan	408:424	After GTP pretreatment and sequential water and organic solvent extraction, up to 80% of the initial xylan remained in the pretreated biomass.
27312636	2	31	theme	xylan	420:424	arg1	%					403:403	up to 80%	395:403	up to 80% of the initial xylan	395:424	After GTP pretreatment and sequential water and organic solvent extraction, up to 80% of the initial xylan remained in the pretreated biomass.
27312636	11	32	theme	weight	1845:1850	arg1	xylan					1852:1856	higher molecular weight xylan	1828:1856	higher molecular weight xylan that may serve value-added applications such as healthcare materials and advanced packaging	1828:1948	This study provided insight into fractionating higher molecular weight xylan that may serve value-added applications such as healthcare materials and advanced packaging.
27312636	0	33	theme	xylan	30:34	arg1	heterogeneity					13:25	the heterogeneity	9:25	the heterogeneity of xylan	9:34	Reducing the heterogeneity of xylan through processing.
27312636	5	34	theme	residual	793:800	arg1	xylan					802:806	The residual xylan	789:806	The residual xylan dissolved in the neutralized filtrate	789:844	The residual xylan dissolved in the neutralized filtrate was precipitated in cold methanol and recovered as the water soluble xylan portion (WSX).
27312636	5	34	theme	residual	793:800	arg1	portion					921:927	the water soluble xylan portion	897:927	the water soluble xylan portion (WSX)	897:933	The residual xylan dissolved in the neutralized filtrate was precipitated in cold methanol and recovered as the water soluble xylan portion (WSX).
27312636	3	35	theme	untreated	652:660	arg1	hardwood					662:669	untreated hardwood	652:669	untreated hardwood	652:669	The majority of the xylan from GTP pretreated and water/solvent extracted biomass was removed using a mild alkali extraction and the composition was compared to xylan directly isolated from untreated hardwood.
27312636	3	36	from	majority	466:473	arg1	water/solvent					512:524	water/solvent	512:524	water/solvent	512:524	The majority of the xylan from GTP pretreated and water/solvent extracted biomass was removed using a mild alkali extraction and the composition was compared to xylan directly isolated from untreated hardwood.
27312636	3	36	from	majority	466:473	arg1	pretreated					497:506	pretreated	497:506	pretreated	497:506	The majority of the xylan from GTP pretreated and water/solvent extracted biomass was removed using a mild alkali extraction and the composition was compared to xylan directly isolated from untreated hardwood.
27312636	2	37	theme	solvent	375:381	arg1	extraction					383:392	organic solvent extraction	367:392	organic solvent extraction	367:392	After GTP pretreatment and sequential water and organic solvent extraction, up to 80% of the initial xylan remained in the pretreated biomass.
27312636	9	38	theme	more	1520:1523	arg1	contamination					1532:1544	more lignin contamination	1520:1544	more lignin contamination	1520:1544	Furthermore, crude WSX contained less xylan with more lignin contamination at severe GTP conditions.
27312636	8	39	contain	contained	1393:1401	arg1	xylan					1368:1372	xylan	1368:1372	xylan isolated after GTP	1368:1391	FT-IR analysis of WIX revealed that xylan isolated after GTP contained peaks related to a reduced carbonyl signal compared to the control.
27312636	8	39	contain	contained	1393:1401	arg2	peaks					1403:1407	peaks	1403:1407	peaks related to a reduced carbonyl signal compared to the control	1403:1468	FT-IR analysis of WIX revealed that xylan isolated after GTP contained peaks related to a reduced carbonyl signal compared to the control.
27312636	3	40	theme	xylan	482:486	arg1	majority					466:473	The majority	462:473	The majority of the xylan from GTP pretreated and water/solvent extracted biomass	462:542	The majority of the xylan from GTP pretreated and water/solvent extracted biomass was removed using a mild alkali extraction and the composition was compared to xylan directly isolated from untreated hardwood.
27312636	8	41	theme	WIX	1350:1352	arg1	analysis					1338:1345	FT-IR analysis	1332:1345	FT-IR analysis of WIX	1332:1352	FT-IR analysis of WIX revealed that xylan isolated after GTP contained peaks related to a reduced carbonyl signal compared to the control.
27312636	3	42	theme	extracted	526:534	arg1	biomass					536:542	extracted biomass	526:542	extracted biomass	526:542	The majority of the xylan from GTP pretreated and water/solvent extracted biomass was removed using a mild alkali extraction and the composition was compared to xylan directly isolated from untreated hardwood.
27312636	1	43	theme	major	241:245	arg1	glucuronoxylan					262:275	glucuronoxylan	262:275	glucuronoxylan	262:275	Glycerol thermal processing (GTP) of hardwood biomass at temperatures between 200 and 240°C facilitated stepwise biopolymer fractionation, while limiting significant degradation of the major hemicellulose, glucuronoxylan, into water-extractable oligosaccharides.
27312636	1	43	theme	major	241:245	arg1	hemicellulose					247:259	the major hemicellulose	237:259	the major hemicellulose	237:259	Glycerol thermal processing (GTP) of hardwood biomass at temperatures between 200 and 240°C facilitated stepwise biopolymer fractionation, while limiting significant degradation of the major hemicellulose, glucuronoxylan, into water-extractable oligosaccharides.
27312636	6	44	theme	average	1088:1094	arg1	DP					1096:1097	a much lower average DP	1075:1097	a much lower average DP of 27 (ca)	1075:1108	Results showed that xylan in WIX was in a polymeric form with a number average degree of polymerization (DP) over 100, whereas the WSX had a much lower average DP of 27 (ca) and contained more substitution.
27312636	9	45	theme	severe	1549:1554	arg1	conditions					1560:1569	severe GTP conditions	1549:1569	severe GTP conditions	1549:1569	Furthermore, crude WSX contained less xylan with more lignin contamination at severe GTP conditions.
27312636	10	46	theme	preferential	1628:1639	arg1	strategy					1654:1661	a preferential purification strategy	1626:1661	a preferential purification strategy resulting in WIX	1626:1678	The recovery of the xylan in two portions facilitated a preferential purification strategy resulting in WIX with an extremely narrow polydispersity index between 1.1 and 1.25, dependent upon the GTP severity.
27312636	9	47	contain	contained	1494:1502	arg2	WSX					1490:1492	crude WSX	1484:1492	crude WSX	1484:1492	Furthermore, crude WSX contained less xylan with more lignin contamination at severe GTP conditions.
27312636	9	47	contain	contained	1494:1502	arg1	WSX					1490:1492	crude WSX	1484:1492	crude WSX	1484:1492	Furthermore, crude WSX contained less xylan with more lignin contamination at severe GTP conditions.
27312636	9	47	contain	contained	1494:1502	arg1	conditions					1560:1569	severe GTP conditions	1549:1569	severe GTP conditions	1549:1569	Furthermore, crude WSX contained less xylan with more lignin contamination at severe GTP conditions.
27312636	9	47	contain	contained	1494:1502	arg2	xylan					1509:1513	less xylan	1504:1513	less xylan	1504:1513	Furthermore, crude WSX contained less xylan with more lignin contamination at severe GTP conditions.
27312636	3	48	from	water/solvent	512:524	arg1	majority					466:473	The majority	462:473	The majority of the xylan from GTP pretreated and water/solvent extracted biomass	462:542	The majority of the xylan from GTP pretreated and water/solvent extracted biomass was removed using a mild alkali extraction and the composition was compared to xylan directly isolated from untreated hardwood.
27312636	3	48	from	water/solvent	512:524	arg1	xylan					482:486	the xylan	478:486	the xylan from GTP pretreated and water/solvent extracted biomass	478:542	The majority of the xylan from GTP pretreated and water/solvent extracted biomass was removed using a mild alkali extraction and the composition was compared to xylan directly isolated from untreated hardwood.
27312636	2	49	theme	sequential	346:355	arg1	water					357:361	sequential water	346:361	sequential water	346:361	After GTP pretreatment and sequential water and organic solvent extraction, up to 80% of the initial xylan remained in the pretreated biomass.
27312636	11	50	theme	advanced	1931:1938	arg1	packaging					1940:1948	advanced packaging	1931:1948	advanced packaging	1931:1948	This study provided insight into fractionating higher molecular weight xylan that may serve value-added applications such as healthcare materials and advanced packaging.
27312636	1	51	theme	stepwise	160:167	arg1	fractionation					180:192	stepwise biopolymer fractionation	160:192	stepwise biopolymer fractionation	160:192	Glycerol thermal processing (GTP) of hardwood biomass at temperatures between 200 and 240°C facilitated stepwise biopolymer fractionation, while limiting significant degradation of the major hemicellulose, glucuronoxylan, into water-extractable oligosaccharides.
27312636	10	52	theme	polydispersity	1705:1718	arg1	index					1720:1724	an extremely narrow polydispersity index	1685:1724	an extremely narrow polydispersity index between 1.1 and 1.25	1685:1745	The recovery of the xylan in two portions facilitated a preferential purification strategy resulting in WIX with an extremely narrow polydispersity index between 1.1 and 1.25, dependent upon the GTP severity.
27312636	3	53	from	pretreated	497:506	arg1	majority					466:473	The majority	462:473	The majority of the xylan from GTP pretreated and water/solvent extracted biomass	462:542	The majority of the xylan from GTP pretreated and water/solvent extracted biomass was removed using a mild alkali extraction and the composition was compared to xylan directly isolated from untreated hardwood.
27312636	3	53	from	pretreated	497:506	arg1	xylan					482:486	the xylan	478:486	the xylan from GTP pretreated and water/solvent extracted biomass	478:542	The majority of the xylan from GTP pretreated and water/solvent extracted biomass was removed using a mild alkali extraction and the composition was compared to xylan directly isolated from untreated hardwood.
27312636	3	54	theme	mild	564:567	arg1	extraction					576:585	a mild alkali extraction	562:585	a mild alkali extraction	562:585	The majority of the xylan from GTP pretreated and water/solvent extracted biomass was removed using a mild alkali extraction and the composition was compared to xylan directly isolated from untreated hardwood.
27312636	6	55	contain	had	1071:1073	arg1	WSX					1067:1069	the WSX	1063:1069	the WSX	1063:1069	Results showed that xylan in WIX was in a polymeric form with a number average degree of polymerization (DP) over 100, whereas the WSX had a much lower average DP of 27 (ca) and contained more substitution.
27312636	6	55	contain	had	1071:1073	arg2	DP					1096:1097	a much lower average DP	1075:1097	a much lower average DP of 27 (ca)	1075:1108	Results showed that xylan in WIX was in a polymeric form with a number average degree of polymerization (DP) over 100, whereas the WSX had a much lower average DP of 27 (ca) and contained more substitution.
27312636	7	56	theme	compositional	1308:1320	arg1	analysis					1322:1329	compositional analysis	1308:1329	compositional analysis	1308:1329	As the processing severity increased during GTP pretreatment, the proportion of WIX increased and the purity of the xylan within the WIX sample reached 84% based on compositional analysis.
27312636	1	57	theme	significant	210:220	arg1	degradation					222:232	significant degradation	210:232	significant degradation of the major hemicellulose, glucuronoxylan,	210:276	Glycerol thermal processing (GTP) of hardwood biomass at temperatures between 200 and 240°C facilitated stepwise biopolymer fractionation, while limiting significant degradation of the major hemicellulose, glucuronoxylan, into water-extractable oligosaccharides.
27312636	1	58	theme	biomass	102:108	arg1	processing					73:82	Glycerol thermal processing	56:82	Glycerol thermal processing (GTP) of hardwood biomass at temperatures between 200 and 240°C	56:146	Glycerol thermal processing (GTP) of hardwood biomass at temperatures between 200 and 240°C facilitated stepwise biopolymer fractionation, while limiting significant degradation of the major hemicellulose, glucuronoxylan, into water-extractable oligosaccharides.
27312636	1	58	theme	biomass	102:108	arg1	GTP					85:87	GTP	85:87	GTP	85:87	Glycerol thermal processing (GTP) of hardwood biomass at temperatures between 200 and 240°C facilitated stepwise biopolymer fractionation, while limiting significant degradation of the major hemicellulose, glucuronoxylan, into water-extractable oligosaccharides.
27312636	5	59	from	precipitated	850:861	arg1	methanol					871:878	cold methanol	866:878	cold methanol	866:878	The residual xylan dissolved in the neutralized filtrate was precipitated in cold methanol and recovered as the water soluble xylan portion (WSX).
27312636	6	60	theme	average	1007:1013	arg1	degree					1015:1020	a number average degree	998:1020	a number average degree of polymerization (DP)	998:1043	Results showed that xylan in WIX was in a polymeric form with a number average degree of polymerization (DP) over 100, whereas the WSX had a much lower average DP of 27 (ca) and contained more substitution.
27312636	7	61	theme	processing	1150:1159	arg1	severity					1161:1168	the processing severity	1146:1168	the processing severity	1146:1168	As the processing severity increased during GTP pretreatment, the proportion of WIX increased and the purity of the xylan within the WIX sample reached 84% based on compositional analysis.
27312636	5	62	theme	soluble	907:913	arg1	xylan					802:806	The residual xylan	789:806	The residual xylan dissolved in the neutralized filtrate	789:844	The residual xylan dissolved in the neutralized filtrate was precipitated in cold methanol and recovered as the water soluble xylan portion (WSX).
27312636	5	62	theme	soluble	907:913	arg1	WSX					930:932	WSX	930:932	WSX	930:932	The residual xylan dissolved in the neutralized filtrate was precipitated in cold methanol and recovered as the water soluble xylan portion (WSX).
27312636	5	62	theme	soluble	907:913	arg1	portion					921:927	the water soluble xylan portion	897:927	the water soluble xylan portion (WSX)	897:933	The residual xylan dissolved in the neutralized filtrate was precipitated in cold methanol and recovered as the water soluble xylan portion (WSX).
27312636	8	63	theme	reduced	1422:1428	arg1	signal					1439:1444	a reduced carbonyl signal	1420:1444	a reduced carbonyl signal compared to the control	1420:1468	FT-IR analysis of WIX revealed that xylan isolated after GTP contained peaks related to a reduced carbonyl signal compared to the control.
27312636	4	64	theme	alkaline	716:723	arg1	filtrate					725:732	the neutralized alkaline filtrate	700:732	the neutralized alkaline filtrate	700:732	The precipitated xylan from the neutralized alkaline filtrate was isolated as a water insoluble xylan portion (WIX).
27312636	2	65	dep	80	401:402	arg1	to					398:399	to	398:399	to	398:399	After GTP pretreatment and sequential water and organic solvent extraction, up to 80% of the initial xylan remained in the pretreated biomass.
27312636	1	66	theme	Glycerol	56:63	arg1	processing					73:82	Glycerol thermal processing	56:82	Glycerol thermal processing (GTP) of hardwood biomass at temperatures between 200 and 240°C	56:146	Glycerol thermal processing (GTP) of hardwood biomass at temperatures between 200 and 240°C facilitated stepwise biopolymer fractionation, while limiting significant degradation of the major hemicellulose, glucuronoxylan, into water-extractable oligosaccharides.
27312636	1	66	theme	Glycerol	56:63	arg1	GTP					85:87	GTP	85:87	GTP	85:87	Glycerol thermal processing (GTP) of hardwood biomass at temperatures between 200 and 240°C facilitated stepwise biopolymer fractionation, while limiting significant degradation of the major hemicellulose, glucuronoxylan, into water-extractable oligosaccharides.
27312636	3	67	theme	alkali	569:574	arg1	extraction					576:585	a mild alkali extraction	562:585	a mild alkali extraction	562:585	The majority of the xylan from GTP pretreated and water/solvent extracted biomass was removed using a mild alkali extraction and the composition was compared to xylan directly isolated from untreated hardwood.
27312636	11	68	theme	molecular	1835:1843	arg1	weight					1845:1850	higher molecular weight	1828:1850	higher molecular weight xylan that may serve value-added applications such as healthcare materials and advanced packaging	1828:1948	This study provided insight into fractionating higher molecular weight xylan that may serve value-added applications such as healthcare materials and advanced packaging.
27312636	4	69	theme	water	752:756	arg1	WIX					783:785	WIX	783:785	WIX	783:785	The precipitated xylan from the neutralized alkaline filtrate was isolated as a water insoluble xylan portion (WIX).
27312636	4	69	theme	water	752:756	arg1	portion					774:780	a water insoluble xylan portion	750:780	a water insoluble xylan portion (WIX)	750:786	The precipitated xylan from the neutralized alkaline filtrate was isolated as a water insoluble xylan portion (WIX).
27312636	4	69	theme	water	752:756	arg1	xylan					689:693	The precipitated xylan	672:693	The precipitated xylan from the neutralized alkaline filtrate	672:732	The precipitated xylan from the neutralized alkaline filtrate was isolated as a water insoluble xylan portion (WIX).
27312636	5	70	theme	neutralized	825:835	arg1	filtrate					837:844	the neutralized filtrate	821:844	the neutralized filtrate	821:844	The residual xylan dissolved in the neutralized filtrate was precipitated in cold methanol and recovered as the water soluble xylan portion (WSX).
27312636	6	71	from	xylan	956:960	arg1	WIX					965:967	WIX	965:967	WIX	965:967	Results showed that xylan in WIX was in a polymeric form with a number average degree of polymerization (DP) over 100, whereas the WSX had a much lower average DP of 27 (ca) and contained more substitution.
27312636	4	72	theme	xylan	768:772	arg1	WIX					783:785	WIX	783:785	WIX	783:785	The precipitated xylan from the neutralized alkaline filtrate was isolated as a water insoluble xylan portion (WIX).
27312636	4	72	theme	xylan	768:772	arg1	portion					774:780	a water insoluble xylan portion	750:780	a water insoluble xylan portion (WIX)	750:786	The precipitated xylan from the neutralized alkaline filtrate was isolated as a water insoluble xylan portion (WIX).
27312636	4	72	theme	xylan	768:772	arg1	xylan					689:693	The precipitated xylan	672:693	The precipitated xylan from the neutralized alkaline filtrate	672:732	The precipitated xylan from the neutralized alkaline filtrate was isolated as a water insoluble xylan portion (WIX).
27312636	7	73	theme	WIX	1223:1225	arg1	proportion					1209:1218	the proportion	1205:1218	the proportion of WIX	1205:1225	As the processing severity increased during GTP pretreatment, the proportion of WIX increased and the purity of the xylan within the WIX sample reached 84% based on compositional analysis.
27312636	6	74	contain	contained	1114:1122	arg1	WSX					1067:1069	the WSX	1063:1069	the WSX	1063:1069	Results showed that xylan in WIX was in a polymeric form with a number average degree of polymerization (DP) over 100, whereas the WSX had a much lower average DP of 27 (ca) and contained more substitution.
27312636	6	74	contain	contained	1114:1122	arg2	substitution					1129:1140	more substitution	1124:1140	more substitution	1124:1140	Results showed that xylan in WIX was in a polymeric form with a number average degree of polymerization (DP) over 100, whereas the WSX had a much lower average DP of 27 (ca) and contained more substitution.
27312636	10	75	theme	GTP	1767:1769	arg1	severity					1771:1778	the GTP severity	1763:1778	the GTP severity	1763:1778	The recovery of the xylan in two portions facilitated a preferential purification strategy resulting in WIX with an extremely narrow polydispersity index between 1.1 and 1.25, dependent upon the GTP severity.
27312636	2	76	theme	initial	412:418	arg1	xylan					420:424	the initial xylan	408:424	the initial xylan	408:424	After GTP pretreatment and sequential water and organic solvent extraction, up to 80% of the initial xylan remained in the pretreated biomass.
27312636	0	77	gly	heterogeneity	13:25	arg1	xylan					30:34	xylan	30:34	xylan	30:34	Reducing the heterogeneity of xylan through processing.
27312636	6	78	theme	lower	1082:1086	arg1	DP					1096:1097	a much lower average DP	1075:1097	a much lower average DP of 27 (ca)	1075:1108	Results showed that xylan in WIX was in a polymeric form with a number average degree of polymerization (DP) over 100, whereas the WSX had a much lower average DP of 27 (ca) and contained more substitution.
27312636	2	79	theme	organic	367:373	arg1	extraction					383:392	organic solvent extraction	367:392	organic solvent extraction	367:392	After GTP pretreatment and sequential water and organic solvent extraction, up to 80% of the initial xylan remained in the pretreated biomass.
27312636	9	80	theme	lignin	1525:1530	arg1	contamination					1532:1544	more lignin contamination	1520:1544	more lignin contamination	1520:1544	Furthermore, crude WSX contained less xylan with more lignin contamination at severe GTP conditions.
27312636	8	81	theme	FT-IR	1332:1336	arg1	analysis					1338:1345	FT-IR analysis	1332:1345	FT-IR analysis of WIX	1332:1352	FT-IR analysis of WIX revealed that xylan isolated after GTP contained peaks related to a reduced carbonyl signal compared to the control.
27312636	10	82	from	recovery	1576:1583	arg1	portions					1605:1612	two portions	1601:1612	two portions	1601:1612	The recovery of the xylan in two portions facilitated a preferential purification strategy resulting in WIX with an extremely narrow polydispersity index between 1.1 and 1.25, dependent upon the GTP severity.
27312636	7	83	theme	GTP	1187:1189	arg1	pretreatment					1191:1202	GTP pretreatment	1187:1202	GTP pretreatment	1187:1202	As the processing severity increased during GTP pretreatment, the proportion of WIX increased and the purity of the xylan within the WIX sample reached 84% based on compositional analysis.
27312636	10	84	theme	purification	1641:1652	arg1	strategy					1654:1661	a preferential purification strategy	1626:1661	a preferential purification strategy resulting in WIX	1626:1678	The recovery of the xylan in two portions facilitated a preferential purification strategy resulting in WIX with an extremely narrow polydispersity index between 1.1 and 1.25, dependent upon the GTP severity.
27312636	4	85	from	filtrate	725:732	arg1	portion					774:780	a water insoluble xylan portion	750:780	a water insoluble xylan portion (WIX)	750:786	The precipitated xylan from the neutralized alkaline filtrate was isolated as a water insoluble xylan portion (WIX).
27312636	4	85	from	filtrate	725:732	arg1	xylan					689:693	The precipitated xylan	672:693	The precipitated xylan from the neutralized alkaline filtrate	672:732	The precipitated xylan from the neutralized alkaline filtrate was isolated as a water insoluble xylan portion (WIX).
27312636	1	86	theme	hemicellulose	247:259	arg1	degradation					222:232	significant degradation	210:232	significant degradation of the major hemicellulose, glucuronoxylan,	210:276	Glycerol thermal processing (GTP) of hardwood biomass at temperatures between 200 and 240°C facilitated stepwise biopolymer fractionation, while limiting significant degradation of the major hemicellulose, glucuronoxylan, into water-extractable oligosaccharides.
25915761	5	0	theme	core	731:734	arg1	glycans					738:744	disialylated core 1 glycans	718:744	disialylated core 1 glycans	718:744	In all cases, the threonines were modified predominantly with disialylated core 1 glycans, together with related core 1 and core 2 structures.
25915761	2	1	gly	O-glycosylated	348:361	arg1	threonine					296:304	the threonine	292:304	the threonine at consensus position 499	292:330	When the gp120s of HIV-1 NL4-3, HIV-1 YU2, HIV-1 Bal, HIV-1 JRFL, and HIV-1 JRCSF were expressed as secreted proteins, the threonine at consensus position 499 was found to be O-glycosylated.
25915761	2	1	gly	O-glycosylated	348:361	arg1	O-glycosylated					348:361	O-glycosylated	348:361	O-glycosylated	348:361	When the gp120s of HIV-1 NL4-3, HIV-1 YU2, HIV-1 Bal, HIV-1 JRFL, and HIV-1 JRCSF were expressed as secreted proteins, the threonine at consensus position 499 was found to be O-glycosylated.
25915761	8	2	from	absence	1213:1219	arg1	Thr499					1250:1255	Thr499	1250:1255	Thr499 as it exists on the surface of virions	1250:1294	Our results indicate the absence of O-linked carbohydrates on Thr499 as it exists on the surface of virions and suggest caution in the interpretation of analyses of post-translational modifications that utilize recombinant forms of envelope protein.
25915761	6	3	gly	monosialylated	864:877	arg1	O-glycans					886:894	mainly monosialylated core 1 O-glycans	857:894	mainly monosialylated core 1 O-glycans	857:894	Secreted HIV-1 gp140 was modified to a lesser extent with mainly monosialylated core 1 O-glycans, suggesting that the ectodomain of the gp41 transmembrane component may limit the accessibility of Thr499 to glycosyltransferases.
25915761	6	4	theme	transmembrane	940:952	arg1	component					954:962	the gp41 transmembrane component	931:962	the gp41 transmembrane component	931:962	Secreted HIV-1 gp140 was modified to a lesser extent with mainly monosialylated core 1 O-glycans, suggesting that the ectodomain of the gp41 transmembrane component may limit the accessibility of Thr499 to glycosyltransferases.
25915761	7	5	theme	C-terminal	1166:1175	arg1	threonine					1177:1185	the C-terminal threonine	1162:1185	the C-terminal threonine	1162:1185	In striking contrast to these findings, gp120 on purified virions of HIV-1 Bal and SIV CP-MAC lacked any detectable O-glycosylation of the C-terminal threonine.
25915761	4	6	theme	Similarly-positioned	469:488	arg1	threonines					508:517	Similarly-positioned, highly-conserved threonines	469:517	Similarly-positioned, highly-conserved threonines in the influenza A virus H1N1 HA1	469:551	Similarly-positioned, highly-conserved threonines in the influenza A virus H1N1 HA1 and H5N1 HA1 envelope proteins were also found to carry O-glycans when expressed as secreted proteins.
25915761	7	7	theme	purified	1076:1083	arg1	virions					1085:1091	purified virions	1076:1091	purified virions of HIV-1 Bal and SIV CP-MAC	1076:1119	In striking contrast to these findings, gp120 on purified virions of HIV-1 Bal and SIV CP-MAC lacked any detectable O-glycosylation of the C-terminal threonine.
25915761	6	8	theme	Secreted	799:806	arg1	gp140					814:818	Secreted HIV-1 gp140	799:818	Secreted HIV-1 gp140	799:818	Secreted HIV-1 gp140 was modified to a lesser extent with mainly monosialylated core 1 O-glycans, suggesting that the ectodomain of the gp41 transmembrane component may limit the accessibility of Thr499 to glycosyltransferases.
25915761	4	9	theme	H1N1	544:547	arg1	HA1					549:551	the influenza A virus H1N1 HA1	522:551	the influenza A virus H1N1 HA1	522:551	Similarly-positioned, highly-conserved threonines in the influenza A virus H1N1 HA1 and H5N1 HA1 envelope proteins were also found to carry O-glycans when expressed as secreted proteins.
25915761	0	10	from	Gp120	0:4	arg1	Virions					15:21	HIV-1 Virions	9:21	HIV-1 Virions	9:21	Gp120 on HIV-1 Virions Lacks O-Linked Carbohydrate.
25915761	7	11	gly	O-glycosylation	1143:1157	arg1	contrast					1039:1046	striking contrast	1030:1046	striking contrast to these findings	1030:1064	In striking contrast to these findings, gp120 on purified virions of HIV-1 Bal and SIV CP-MAC lacked any detectable O-glycosylation of the C-terminal threonine.
25915761	7	11	gly	O-glycosylation	1143:1157	arg1	threonine					1177:1185	the C-terminal threonine	1162:1185	the C-terminal threonine	1162:1185	In striking contrast to these findings, gp120 on purified virions of HIV-1 Bal and SIV CP-MAC lacked any detectable O-glycosylation of the C-terminal threonine.
25915761	5	12	gly	disialylated	718:729	arg1	glycans					738:744	disialylated core 1 glycans	718:744	disialylated core 1 glycans	718:744	In all cases, the threonines were modified predominantly with disialylated core 1 glycans, together with related core 1 and core 2 structures.
25915761	3	13	gly	glycosylated	416:427	arg1	threonine					397:405	the corresponding threonine	379:405	the corresponding threonine	379:405	For SIVmac239, the corresponding threonine was also glycosylated when gp120 was recombinantly expressed.
25915761	4	14	theme	H5N1	557:560	arg1	proteins					575:582	H5N1 HA1 envelope proteins	557:582	H5N1 HA1 envelope proteins	557:582	Similarly-positioned, highly-conserved threonines in the influenza A virus H1N1 HA1 and H5N1 HA1 envelope proteins were also found to carry O-glycans when expressed as secreted proteins.
25915761	5	15	mod	modified	690:697	arg3	glycans					738:744	disialylated core 1 glycans	718:744	disialylated core 1 glycans	718:744	In all cases, the threonines were modified predominantly with disialylated core 1 glycans, together with related core 1 and core 2 structures.
25915761	5	15	mod	modified	690:697	arg1	threonines					674:683	the threonines	670:683	the threonines	670:683	In all cases, the threonines were modified predominantly with disialylated core 1 glycans, together with related core 1 and core 2 structures.
25915761	6	16	theme	lesser	838:843	arg1	extent					845:850	a lesser extent	836:850	a lesser extent	836:850	Secreted HIV-1 gp140 was modified to a lesser extent with mainly monosialylated core 1 O-glycans, suggesting that the ectodomain of the gp41 transmembrane component may limit the accessibility of Thr499 to glycosyltransferases.
25915761	5	17	dep	core	769:772	arg1	structures					787:796	structures	787:796	structures	787:796	In all cases, the threonines were modified predominantly with disialylated core 1 glycans, together with related core 1 and core 2 structures.
25915761	8	18	from	Thr499	1250:1255	arg1	absence					1213:1219	the absence	1209:1219	the absence of O-linked carbohydrates on Thr499 as it exists on the surface of virions	1209:1294	Our results indicate the absence of O-linked carbohydrates on Thr499 as it exists on the surface of virions and suggest caution in the interpretation of analyses of post-translational modifications that utilize recombinant forms of envelope protein.
25915761	1	19	theme	HIV-1-encoded	55:67	arg1	protein					78:84	HIV-1-encoded envelope protein	55:84	HIV-1-encoded envelope protein	55:84	As HIV-1-encoded envelope protein traverses the secretory pathway, it may be modified with N- and O-linked carbohydrate.
25915761	1	20	theme	envelope	69:76	arg1	protein					78:84	HIV-1-encoded envelope protein	55:84	HIV-1-encoded envelope protein	55:84	As HIV-1-encoded envelope protein traverses the secretory pathway, it may be modified with N- and O-linked carbohydrate.
25915761	1	21	theme	O-linked	150:157	arg1	carbohydrate					159:170	O-linked carbohydrate	150:170	O-linked carbohydrate	150:170	As HIV-1-encoded envelope protein traverses the secretory pathway, it may be modified with N- and O-linked carbohydrate.
25915761	7	22	theme	detectable	1132:1141	arg1	O-glycosylation					1143:1157	any detectable O-glycosylation	1128:1157	any detectable O-glycosylation of the C-terminal threonine	1128:1185	In striking contrast to these findings, gp120 on purified virions of HIV-1 Bal and SIV CP-MAC lacked any detectable O-glycosylation of the C-terminal threonine.
25915761	7	23	theme	HIV-1	1096:1100	arg1	virions					1085:1091	purified virions	1076:1091	purified virions of HIV-1 Bal and SIV CP-MAC	1076:1119	In striking contrast to these findings, gp120 on purified virions of HIV-1 Bal and SIV CP-MAC lacked any detectable O-glycosylation of the C-terminal threonine.
25915761	7	24	theme	striking	1030:1037	arg1	contrast					1039:1046	striking contrast	1030:1046	striking contrast to these findings	1030:1064	In striking contrast to these findings, gp120 on purified virions of HIV-1 Bal and SIV CP-MAC lacked any detectable O-glycosylation of the C-terminal threonine.
25915761	5	25	theme	related	761:767	arg1	core					769:772	related core 1	761:774	related core 1	761:774	In all cases, the threonines were modified predominantly with disialylated core 1 glycans, together with related core 1 and core 2 structures.
25915761	8	26	theme	modifications	1372:1384	arg1	analyses					1341:1348	analyses	1341:1348	analyses of post-translational modifications that utilize recombinant forms of envelope protein	1341:1435	Our results indicate the absence of O-linked carbohydrates on Thr499 as it exists on the surface of virions and suggest caution in the interpretation of analyses of post-translational modifications that utilize recombinant forms of envelope protein.
25915761	1	27	link	O-linked	150:157	arg1	carbohydrate					159:170	O-linked carbohydrate	150:170	O-linked carbohydrate	150:170	As HIV-1-encoded envelope protein traverses the secretory pathway, it may be modified with N- and O-linked carbohydrate.
25915761	4	28	theme	influenza	526:534	arg1	HA1					549:551	the influenza A virus H1N1 HA1	522:551	the influenza A virus H1N1 HA1	522:551	Similarly-positioned, highly-conserved threonines in the influenza A virus H1N1 HA1 and H5N1 HA1 envelope proteins were also found to carry O-glycans when expressed as secreted proteins.
25915761	7	29	dep	HIV-1	1096:1100	arg1	Bal					1102:1104	Bal	1102:1104	Bal	1102:1104	In striking contrast to these findings, gp120 on purified virions of HIV-1 Bal and SIV CP-MAC lacked any detectable O-glycosylation of the C-terminal threonine.
25915761	7	29	dep	HIV-1	1096:1100	arg1	CP-MAC					1114:1119	CP-MAC	1114:1119	CP-MAC	1114:1119	In striking contrast to these findings, gp120 on purified virions of HIV-1 Bal and SIV CP-MAC lacked any detectable O-glycosylation of the C-terminal threonine.
25915761	8	30	theme	recombinant	1399:1409	arg1	forms					1411:1415	recombinant forms	1399:1415	recombinant forms of envelope protein	1399:1435	Our results indicate the absence of O-linked carbohydrates on Thr499 as it exists on the surface of virions and suggest caution in the interpretation of analyses of post-translational modifications that utilize recombinant forms of envelope protein.
25915761	8	31	theme	envelope	1420:1427	arg1	protein					1429:1435	envelope protein	1420:1435	envelope protein	1420:1435	Our results indicate the absence of O-linked carbohydrates on Thr499 as it exists on the surface of virions and suggest caution in the interpretation of analyses of post-translational modifications that utilize recombinant forms of envelope protein.
25915761	2	32	theme	JRCSF	249:253	arg1	proteins					282:289	secreted proteins	273:289	secreted proteins	273:289	When the gp120s of HIV-1 NL4-3, HIV-1 YU2, HIV-1 Bal, HIV-1 JRFL, and HIV-1 JRCSF were expressed as secreted proteins, the threonine at consensus position 499 was found to be O-glycosylated.
25915761	2	32	theme	JRCSF	249:253	arg1	gp120s					182:187	the gp120s	178:187	the gp120s of HIV-1 NL4-3, HIV-1 YU2, HIV-1 Bal, HIV-1 JRFL, and HIV-1 JRCSF	178:253	When the gp120s of HIV-1 NL4-3, HIV-1 YU2, HIV-1 Bal, HIV-1 JRFL, and HIV-1 JRCSF were expressed as secreted proteins, the threonine at consensus position 499 was found to be O-glycosylated.
25915761	8	33	theme	analyses	1341:1348	arg1	interpretation					1323:1336	the interpretation	1319:1336	the interpretation of analyses of post-translational modifications that utilize recombinant forms of envelope protein	1319:1435	Our results indicate the absence of O-linked carbohydrates on Thr499 as it exists on the surface of virions and suggest caution in the interpretation of analyses of post-translational modifications that utilize recombinant forms of envelope protein.
25915761	8	34	theme	virions	1288:1294	arg1	surface					1277:1283	the surface	1273:1283	the surface of virions	1273:1294	Our results indicate the absence of O-linked carbohydrates on Thr499 as it exists on the surface of virions and suggest caution in the interpretation of analyses of post-translational modifications that utilize recombinant forms of envelope protein.
25915761	0	35	theme	HIV-1	9:13	arg1	Virions					15:21	HIV-1 Virions	9:21	HIV-1 Virions	9:21	Gp120 on HIV-1 Virions Lacks O-Linked Carbohydrate.
25915761	4	36	theme	virus	538:542	arg1	HA1					549:551	the influenza A virus H1N1 HA1	522:551	the influenza A virus H1N1 HA1	522:551	Similarly-positioned, highly-conserved threonines in the influenza A virus H1N1 HA1 and H5N1 HA1 envelope proteins were also found to carry O-glycans when expressed as secreted proteins.
25915761	2	37	theme	JRFL	233:236	arg1	proteins					282:289	secreted proteins	273:289	secreted proteins	273:289	When the gp120s of HIV-1 NL4-3, HIV-1 YU2, HIV-1 Bal, HIV-1 JRFL, and HIV-1 JRCSF were expressed as secreted proteins, the threonine at consensus position 499 was found to be O-glycosylated.
25915761	2	37	theme	JRFL	233:236	arg1	gp120s					182:187	the gp120s	178:187	the gp120s of HIV-1 NL4-3, HIV-1 YU2, HIV-1 Bal, HIV-1 JRFL, and HIV-1 JRCSF	178:253	When the gp120s of HIV-1 NL4-3, HIV-1 YU2, HIV-1 Bal, HIV-1 JRFL, and HIV-1 JRCSF were expressed as secreted proteins, the threonine at consensus position 499 was found to be O-glycosylated.
25915761	2	38	theme	HIV-1	243:247	arg1	JRCSF					249:253	HIV-1 JRCSF	243:253	HIV-1 JRCSF	243:253	When the gp120s of HIV-1 NL4-3, HIV-1 YU2, HIV-1 Bal, HIV-1 JRFL, and HIV-1 JRCSF were expressed as secreted proteins, the threonine at consensus position 499 was found to be O-glycosylated.
25915761	6	39	theme	Thr499	995:1000	arg1	accessibility					978:990	the accessibility	974:990	the accessibility of Thr499 to glycosyltransferases	974:1024	Secreted HIV-1 gp140 was modified to a lesser extent with mainly monosialylated core 1 O-glycans, suggesting that the ectodomain of the gp41 transmembrane component may limit the accessibility of Thr499 to glycosyltransferases.
25915761	0	40	theme	O-Linked	29:36	arg1	Carbohydrate					38:49	O-Linked Carbohydrate	29:49	O-Linked Carbohydrate	29:49	Gp120 on HIV-1 Virions Lacks O-Linked Carbohydrate.
25915761	4	41	theme	A	536:536	arg1	HA1					549:551	the influenza A virus H1N1 HA1	522:551	the influenza A virus H1N1 HA1	522:551	Similarly-positioned, highly-conserved threonines in the influenza A virus H1N1 HA1 and H5N1 HA1 envelope proteins were also found to carry O-glycans when expressed as secreted proteins.
25915761	8	42	from	caution	1308:1314	arg1	interpretation					1323:1336	the interpretation	1319:1336	the interpretation of analyses of post-translational modifications that utilize recombinant forms of envelope protein	1319:1435	Our results indicate the absence of O-linked carbohydrates on Thr499 as it exists on the surface of virions and suggest caution in the interpretation of analyses of post-translational modifications that utilize recombinant forms of envelope protein.
25915761	4	43	contain	carry	603:607	arg1	threonines					508:517	Similarly-positioned, highly-conserved threonines	469:517	Similarly-positioned, highly-conserved threonines in the influenza A virus H1N1 HA1	469:551	Similarly-positioned, highly-conserved threonines in the influenza A virus H1N1 HA1 and H5N1 HA1 envelope proteins were also found to carry O-glycans when expressed as secreted proteins.
25915761	4	43	contain	carry	603:607	arg1	proteins					575:582	H5N1 HA1 envelope proteins	557:582	H5N1 HA1 envelope proteins	557:582	Similarly-positioned, highly-conserved threonines in the influenza A virus H1N1 HA1 and H5N1 HA1 envelope proteins were also found to carry O-glycans when expressed as secreted proteins.
25915761	4	43	contain	carry	603:607	arg2	O-glycans					609:617	O-glycans	609:617	O-glycans	609:617	Similarly-positioned, highly-conserved threonines in the influenza A virus H1N1 HA1 and H5N1 HA1 envelope proteins were also found to carry O-glycans when expressed as secreted proteins.
25915761	2	44	theme	YU2	211:213	arg1	proteins					282:289	secreted proteins	273:289	secreted proteins	273:289	When the gp120s of HIV-1 NL4-3, HIV-1 YU2, HIV-1 Bal, HIV-1 JRFL, and HIV-1 JRCSF were expressed as secreted proteins, the threonine at consensus position 499 was found to be O-glycosylated.
25915761	2	44	theme	YU2	211:213	arg1	gp120s					182:187	the gp120s	178:187	the gp120s of HIV-1 NL4-3, HIV-1 YU2, HIV-1 Bal, HIV-1 JRFL, and HIV-1 JRCSF	178:253	When the gp120s of HIV-1 NL4-3, HIV-1 YU2, HIV-1 Bal, HIV-1 JRFL, and HIV-1 JRCSF were expressed as secreted proteins, the threonine at consensus position 499 was found to be O-glycosylated.
25915761	3	45	theme	corresponding	383:395	arg1	threonine					397:405	the corresponding threonine	379:405	the corresponding threonine	379:405	For SIVmac239, the corresponding threonine was also glycosylated when gp120 was recombinantly expressed.
25915761	4	46	from	threonines	508:517	arg1	HA1					549:551	the influenza A virus H1N1 HA1	522:551	the influenza A virus H1N1 HA1	522:551	Similarly-positioned, highly-conserved threonines in the influenza A virus H1N1 HA1 and H5N1 HA1 envelope proteins were also found to carry O-glycans when expressed as secreted proteins.
25915761	4	47	dep	Similarly-positioned	469:488	arg1	highly-conserved					491:506	highly-conserved	491:506	highly-conserved	491:506	Similarly-positioned, highly-conserved threonines in the influenza A virus H1N1 HA1 and H5N1 HA1 envelope proteins were also found to carry O-glycans when expressed as secreted proteins.
25915761	6	48	theme	gp41	935:938	arg1	component					954:962	the gp41 transmembrane component	931:962	the gp41 transmembrane component	931:962	Secreted HIV-1 gp140 was modified to a lesser extent with mainly monosialylated core 1 O-glycans, suggesting that the ectodomain of the gp41 transmembrane component may limit the accessibility of Thr499 to glycosyltransferases.
25915761	6	49	theme	HIV-1	808:812	arg1	gp140					814:818	Secreted HIV-1 gp140	799:818	Secreted HIV-1 gp140	799:818	Secreted HIV-1 gp140 was modified to a lesser extent with mainly monosialylated core 1 O-glycans, suggesting that the ectodomain of the gp41 transmembrane component may limit the accessibility of Thr499 to glycosyltransferases.
25915761	2	50	theme	HIV-1	205:209	arg1	YU2					211:213	HIV-1 YU2	205:213	HIV-1 YU2	205:213	When the gp120s of HIV-1 NL4-3, HIV-1 YU2, HIV-1 Bal, HIV-1 JRFL, and HIV-1 JRCSF were expressed as secreted proteins, the threonine at consensus position 499 was found to be O-glycosylated.
25915761	1	51	mod	modified	129:136	arg3	carbohydrate					159:170	O-linked carbohydrate	150:170	O-linked carbohydrate	150:170	As HIV-1-encoded envelope protein traverses the secretory pathway, it may be modified with N- and O-linked carbohydrate.
25915761	1	51	mod	modified	129:136	arg1	it					119:120	it	119:120	it	119:120	As HIV-1-encoded envelope protein traverses the secretory pathway, it may be modified with N- and O-linked carbohydrate.
25915761	1	51	mod	modified	129:136	arg3	N-					143:144	N-	143:144	N-	143:144	As HIV-1-encoded envelope protein traverses the secretory pathway, it may be modified with N- and O-linked carbohydrate.
25915761	7	52	from	gp120	1067:1071	arg1	virions					1085:1091	purified virions	1076:1091	purified virions of HIV-1 Bal and SIV CP-MAC	1076:1119	In striking contrast to these findings, gp120 on purified virions of HIV-1 Bal and SIV CP-MAC lacked any detectable O-glycosylation of the C-terminal threonine.
25915761	6	53	mod	modified	824:831	arg3	O-glycans					886:894	mainly monosialylated core 1 O-glycans	857:894	mainly monosialylated core 1 O-glycans	857:894	Secreted HIV-1 gp140 was modified to a lesser extent with mainly monosialylated core 1 O-glycans, suggesting that the ectodomain of the gp41 transmembrane component may limit the accessibility of Thr499 to glycosyltransferases.
25915761	6	53	mod	modified	824:831	arg1	gp140					814:818	Secreted HIV-1 gp140	799:818	Secreted HIV-1 gp140	799:818	Secreted HIV-1 gp140 was modified to a lesser extent with mainly monosialylated core 1 O-glycans, suggesting that the ectodomain of the gp41 transmembrane component may limit the accessibility of Thr499 to glycosyltransferases.
25915761	5	54	theme	disialylated	718:729	arg1	glycans					738:744	disialylated core 1 glycans	718:744	disialylated core 1 glycans	718:744	In all cases, the threonines were modified predominantly with disialylated core 1 glycans, together with related core 1 and core 2 structures.
25915761	8	55	theme	post-translational	1353:1370	arg1	modifications					1372:1384	post-translational modifications	1353:1384	post-translational modifications that utilize recombinant forms of envelope protein	1353:1435	Our results indicate the absence of O-linked carbohydrates on Thr499 as it exists on the surface of virions and suggest caution in the interpretation of analyses of post-translational modifications that utilize recombinant forms of envelope protein.
25915761	2	56	from	position	319:326	arg1	threonine					296:304	the threonine	292:304	the threonine at consensus position 499	292:330	When the gp120s of HIV-1 NL4-3, HIV-1 YU2, HIV-1 Bal, HIV-1 JRFL, and HIV-1 JRCSF were expressed as secreted proteins, the threonine at consensus position 499 was found to be O-glycosylated.
25915761	2	56	from	position	319:326	arg1	O-glycosylated					348:361	O-glycosylated	348:361	O-glycosylated	348:361	When the gp120s of HIV-1 NL4-3, HIV-1 YU2, HIV-1 Bal, HIV-1 JRFL, and HIV-1 JRCSF were expressed as secreted proteins, the threonine at consensus position 499 was found to be O-glycosylated.
25915761	2	57	theme	HIV-1	192:196	arg1	NL4-3					198:202	HIV-1 NL4-3	192:202	HIV-1 NL4-3	192:202	When the gp120s of HIV-1 NL4-3, HIV-1 YU2, HIV-1 Bal, HIV-1 JRFL, and HIV-1 JRCSF were expressed as secreted proteins, the threonine at consensus position 499 was found to be O-glycosylated.
25915761	2	58	theme	NL4-3	198:202	arg1	proteins					282:289	secreted proteins	273:289	secreted proteins	273:289	When the gp120s of HIV-1 NL4-3, HIV-1 YU2, HIV-1 Bal, HIV-1 JRFL, and HIV-1 JRCSF were expressed as secreted proteins, the threonine at consensus position 499 was found to be O-glycosylated.
25915761	2	58	theme	NL4-3	198:202	arg1	gp120s					182:187	the gp120s	178:187	the gp120s of HIV-1 NL4-3, HIV-1 YU2, HIV-1 Bal, HIV-1 JRFL, and HIV-1 JRCSF	178:253	When the gp120s of HIV-1 NL4-3, HIV-1 YU2, HIV-1 Bal, HIV-1 JRFL, and HIV-1 JRCSF were expressed as secreted proteins, the threonine at consensus position 499 was found to be O-glycosylated.
25915761	8	59	theme	protein	1429:1435	arg1	forms					1411:1415	recombinant forms	1399:1415	recombinant forms of envelope protein	1399:1435	Our results indicate the absence of O-linked carbohydrates on Thr499 as it exists on the surface of virions and suggest caution in the interpretation of analyses of post-translational modifications that utilize recombinant forms of envelope protein.
25915761	6	60	theme	core	879:882	arg1	O-glycans					886:894	mainly monosialylated core 1 O-glycans	857:894	mainly monosialylated core 1 O-glycans	857:894	Secreted HIV-1 gp140 was modified to a lesser extent with mainly monosialylated core 1 O-glycans, suggesting that the ectodomain of the gp41 transmembrane component may limit the accessibility of Thr499 to glycosyltransferases.
25915761	8	61	theme	carbohydrates	1233:1245	arg1	absence					1213:1219	the absence	1209:1219	the absence of O-linked carbohydrates on Thr499 as it exists on the surface of virions	1209:1294	Our results indicate the absence of O-linked carbohydrates on Thr499 as it exists on the surface of virions and suggest caution in the interpretation of analyses of post-translational modifications that utilize recombinant forms of envelope protein.
25915761	8	62	link	O-linked	1224:1231	arg1	carbohydrates					1233:1245	O-linked carbohydrates	1224:1245	O-linked carbohydrates on Thr499 as it exists on the surface of virions	1224:1294	Our results indicate the absence of O-linked carbohydrates on Thr499 as it exists on the surface of virions and suggest caution in the interpretation of analyses of post-translational modifications that utilize recombinant forms of envelope protein.
25915761	2	63	theme	secreted	273:280	arg1	proteins					282:289	secreted proteins	273:289	secreted proteins	273:289	When the gp120s of HIV-1 NL4-3, HIV-1 YU2, HIV-1 Bal, HIV-1 JRFL, and HIV-1 JRCSF were expressed as secreted proteins, the threonine at consensus position 499 was found to be O-glycosylated.
25915761	2	63	theme	secreted	273:280	arg1	gp120s					182:187	the gp120s	178:187	the gp120s of HIV-1 NL4-3, HIV-1 YU2, HIV-1 Bal, HIV-1 JRFL, and HIV-1 JRCSF	178:253	When the gp120s of HIV-1 NL4-3, HIV-1 YU2, HIV-1 Bal, HIV-1 JRFL, and HIV-1 JRCSF were expressed as secreted proteins, the threonine at consensus position 499 was found to be O-glycosylated.
25915761	1	64	theme	secretory	100:108	arg1	pathway					110:116	the secretory pathway	96:116	the secretory pathway	96:116	As HIV-1-encoded envelope protein traverses the secretory pathway, it may be modified with N- and O-linked carbohydrate.
25915761	4	65	theme	envelope	566:573	arg1	proteins					575:582	H5N1 HA1 envelope proteins	557:582	H5N1 HA1 envelope proteins	557:582	Similarly-positioned, highly-conserved threonines in the influenza A virus H1N1 HA1 and H5N1 HA1 envelope proteins were also found to carry O-glycans when expressed as secreted proteins.
25915761	2	66	theme	Bal	222:224	arg1	proteins					282:289	secreted proteins	273:289	secreted proteins	273:289	When the gp120s of HIV-1 NL4-3, HIV-1 YU2, HIV-1 Bal, HIV-1 JRFL, and HIV-1 JRCSF were expressed as secreted proteins, the threonine at consensus position 499 was found to be O-glycosylated.
25915761	2	66	theme	Bal	222:224	arg1	gp120s					182:187	the gp120s	178:187	the gp120s of HIV-1 NL4-3, HIV-1 YU2, HIV-1 Bal, HIV-1 JRFL, and HIV-1 JRCSF	178:253	When the gp120s of HIV-1 NL4-3, HIV-1 YU2, HIV-1 Bal, HIV-1 JRFL, and HIV-1 JRCSF were expressed as secreted proteins, the threonine at consensus position 499 was found to be O-glycosylated.
25915761	8	67	from	carbohydrates	1233:1245	arg1	Thr499					1250:1255	Thr499	1250:1255	Thr499 as it exists on the surface of virions	1250:1294	Our results indicate the absence of O-linked carbohydrates on Thr499 as it exists on the surface of virions and suggest caution in the interpretation of analyses of post-translational modifications that utilize recombinant forms of envelope protein.
25915761	4	68	theme	HA1	562:564	arg1	proteins					575:582	H5N1 HA1 envelope proteins	557:582	H5N1 HA1 envelope proteins	557:582	Similarly-positioned, highly-conserved threonines in the influenza A virus H1N1 HA1 and H5N1 HA1 envelope proteins were also found to carry O-glycans when expressed as secreted proteins.
25915761	7	69	theme	threonine	1177:1185	arg1	O-glycosylation					1143:1157	any detectable O-glycosylation	1128:1157	any detectable O-glycosylation of the C-terminal threonine	1128:1185	In striking contrast to these findings, gp120 on purified virions of HIV-1 Bal and SIV CP-MAC lacked any detectable O-glycosylation of the C-terminal threonine.
25915761	8	70	theme	O-linked	1224:1231	arg1	carbohydrates					1233:1245	O-linked carbohydrates	1224:1245	O-linked carbohydrates on Thr499 as it exists on the surface of virions	1224:1294	Our results indicate the absence of O-linked carbohydrates on Thr499 as it exists on the surface of virions and suggest caution in the interpretation of analyses of post-translational modifications that utilize recombinant forms of envelope protein.
25915761	2	71	theme	HIV-1	216:220	arg1	Bal					222:224	HIV-1 Bal	216:224	HIV-1 Bal	216:224	When the gp120s of HIV-1 NL4-3, HIV-1 YU2, HIV-1 Bal, HIV-1 JRFL, and HIV-1 JRCSF were expressed as secreted proteins, the threonine at consensus position 499 was found to be O-glycosylated.
25915761	2	72	theme	consensus	309:317	arg1	position					319:326	consensus position 499	309:330	consensus position 499	309:330	When the gp120s of HIV-1 NL4-3, HIV-1 YU2, HIV-1 Bal, HIV-1 JRFL, and HIV-1 JRCSF were expressed as secreted proteins, the threonine at consensus position 499 was found to be O-glycosylated.
25915761	4	73	from	proteins	575:582	arg1	HA1					549:551	the influenza A virus H1N1 HA1	522:551	the influenza A virus H1N1 HA1	522:551	Similarly-positioned, highly-conserved threonines in the influenza A virus H1N1 HA1 and H5N1 HA1 envelope proteins were also found to carry O-glycans when expressed as secreted proteins.
25915761	6	74	theme	monosialylated	864:877	arg1	O-glycans					886:894	mainly monosialylated core 1 O-glycans	857:894	mainly monosialylated core 1 O-glycans	857:894	Secreted HIV-1 gp140 was modified to a lesser extent with mainly monosialylated core 1 O-glycans, suggesting that the ectodomain of the gp41 transmembrane component may limit the accessibility of Thr499 to glycosyltransferases.
25915761	7	75	theme	SIV	1110:1112	arg1	virions					1085:1091	purified virions	1076:1091	purified virions of HIV-1 Bal and SIV CP-MAC	1076:1119	In striking contrast to these findings, gp120 on purified virions of HIV-1 Bal and SIV CP-MAC lacked any detectable O-glycosylation of the C-terminal threonine.
25915761	4	76	theme	secreted	637:644	arg1	proteins					646:653	secreted proteins	637:653	secreted proteins	637:653	Similarly-positioned, highly-conserved threonines in the influenza A virus H1N1 HA1 and H5N1 HA1 envelope proteins were also found to carry O-glycans when expressed as secreted proteins.
25915761	6	77	theme	component	954:962	arg1	component					954:962	the gp41 transmembrane component	931:962	the gp41 transmembrane component	931:962	Secreted HIV-1 gp140 was modified to a lesser extent with mainly monosialylated core 1 O-glycans, suggesting that the ectodomain of the gp41 transmembrane component may limit the accessibility of Thr499 to glycosyltransferases.
25915761	6	77	theme	component	954:962	arg1	ectodomain					917:926	the ectodomain	913:926	the ectodomain of the gp41 transmembrane component	913:962	Secreted HIV-1 gp140 was modified to a lesser extent with mainly monosialylated core 1 O-glycans, suggesting that the ectodomain of the gp41 transmembrane component may limit the accessibility of Thr499 to glycosyltransferases.
25723711	0	0	theme	foams	82:86	arg1	flammability					44:55	the flammability	40:55	the flammability of flexible polyurethane foams	40:86	One-pot, bioinspired coatings to reduce the flammability of flexible polyurethane foams.
25723711	3	1	theme	release	572:578	arg1	value					551:555	a critical flammability value	527:555	a critical flammability value	527:555	Nearly all coatings produced a 63% reduction in a critical flammability value, the peak heat release rate (PHRR).
25723711	3	1	theme	release	572:578	arg1	PHRR					586:589	PHRR	586:589	PHRR	586:589	Nearly all coatings produced a 63% reduction in a critical flammability value, the peak heat release rate (PHRR).
25723711	3	1	theme	release	572:578	arg1	rate					580:583	the peak heat release rate	558:583	the peak heat release rate (PHRR)	558:590	Nearly all coatings produced a 63% reduction in a critical flammability value, the peak heat release rate (PHRR).
25723711	7	2	theme	fire	1061:1064	arg1	retardant					1066:1074	the boron fire retardant	1051:1074	the boron fire retardant	1051:1074	This flammability reduction was caused by three mechanisms-the gas-phase and condensed-phase processes of the boron fire retardant and the condensed-phase process of the clay.
25723711	1	3	theme	bioinspired	209:219	arg1	coatings					221:228	flexible, highly fire resistant, and bioinspired coatings	172:228	flexible, highly fire resistant, and bioinspired coatings on flexible polyurethane foam (PUF)	172:264	In this manuscript, natural materials were combined into a single "pot" to produce flexible, highly fire resistant, and bioinspired coatings on flexible polyurethane foam (PUF).
25723711	0	4	theme	polyurethane	69:80	arg1	foams					82:86	flexible polyurethane foams	60:86	flexible polyurethane foams	60:86	One-pot, bioinspired coatings to reduce the flammability of flexible polyurethane foams.
25723711	4	5	from	reduction	624:632	arg1	PHRR					637:640	PHRR	637:640	PHRR	637:640	One formulation produced a 75% reduction in PHRR.
25723711	1	6	theme	single	148:153	arg1	"					159:159	a single "pot"	146:159	a single "pot" to produce flexible, highly fire resistant, and bioinspired coatings on flexible polyurethane foam (PUF)	146:264	In this manuscript, natural materials were combined into a single "pot" to produce flexible, highly fire resistant, and bioinspired coatings on flexible polyurethane foam (PUF).
25723711	3	7	theme	peak	562:565	arg1	value					551:555	a critical flammability value	527:555	a critical flammability value	527:555	Nearly all coatings produced a 63% reduction in a critical flammability value, the peak heat release rate (PHRR).
25723711	3	7	theme	peak	562:565	arg1	PHRR					586:589	PHRR	586:589	PHRR	586:589	Nearly all coatings produced a 63% reduction in a critical flammability value, the peak heat release rate (PHRR).
25723711	3	7	theme	peak	562:565	arg1	rate					580:583	the peak heat release rate	558:583	the peak heat release rate (PHRR)	558:590	Nearly all coatings produced a 63% reduction in a critical flammability value, the peak heat release rate (PHRR).
25723711	8	8	theme	composition	1191:1201	arg1	impact					1169:1174	the impact	1165:1174	the impact of the coating composition on flammability	1165:1217	We describe the one-pot coating process and the impact of the coating composition on flammability.
25723711	8	8	theme	composition	1191:1201	arg1	process					1153:1159	process	1153:1159	process	1153:1159	We describe the one-pot coating process and the impact of the coating composition on flammability.
25723711	6	9	theme	fire	808:811	arg1	threat					813:818	the fire threat	804:818	the fire threat of furniture from significant fire damage in and beyond the room of fire origin to being contained to the burning furniture	804:942	At these PHRR values, this technology could reduce the fire threat of furniture from significant fire damage in and beyond the room of fire origin to being contained to the burning furniture.
25723711	3	10	from	reduction	514:522	arg1	value					551:555	a critical flammability value	527:555	a critical flammability value	527:555	Nearly all coatings produced a 63% reduction in a critical flammability value, the peak heat release rate (PHRR).
25723711	3	10	from	reduction	514:522	arg1	rate					580:583	the peak heat release rate	558:583	the peak heat release rate (PHRR)	558:590	Nearly all coatings produced a 63% reduction in a critical flammability value, the peak heat release rate (PHRR).
25723711	7	11	theme	retardant	1066:1074	arg1	process					1100:1106	the condensed-phase process	1080:1106	the condensed-phase process of the clay	1080:1118	This flammability reduction was caused by three mechanisms-the gas-phase and condensed-phase processes of the boron fire retardant and the condensed-phase process of the clay.
25723711	7	11	theme	retardant	1066:1074	arg1	processes					1038:1046	three mechanisms-the gas-phase and condensed-phase processes	987:1046	three mechanisms-the gas-phase and condensed-phase processes of the boron fire retardant	987:1074	This flammability reduction was caused by three mechanisms-the gas-phase and condensed-phase processes of the boron fire retardant and the condensed-phase process of the clay.
25723711	1	12	theme	flexible	233:240	arg1	PUF					261:263	flexible polyurethane foam (PUF)	233:264	flexible polyurethane foam (PUF)	233:264	In this manuscript, natural materials were combined into a single "pot" to produce flexible, highly fire resistant, and bioinspired coatings on flexible polyurethane foam (PUF).
25723711	7	13	theme	mechanisms-the	993:1006	arg1	processes					1038:1046	three mechanisms-the gas-phase and condensed-phase processes	987:1046	three mechanisms-the gas-phase and condensed-phase processes of the boron fire retardant	987:1074	This flammability reduction was caused by three mechanisms-the gas-phase and condensed-phase processes of the boron fire retardant and the condensed-phase process of the clay.
25723711	5	14	theme	furniture	687:695	arg1	tests					702:706	full-scale furniture fire tests	676:706	full-scale furniture fire tests	676:706	This technology was validated in full-scale furniture fire tests, where a 75% reduction in PHRR was measured.
25723711	6	15	theme	fire	888:891	arg1	origin					893:898	fire origin	888:898	fire origin	888:898	At these PHRR values, this technology could reduce the fire threat of furniture from significant fire damage in and beyond the room of fire origin to being contained to the burning furniture.
25723711	7	16	theme	gas-phase	1008:1016	arg1	processes					1038:1046	three mechanisms-the gas-phase and condensed-phase processes	987:1046	three mechanisms-the gas-phase and condensed-phase processes of the boron fire retardant	987:1074	This flammability reduction was caused by three mechanisms-the gas-phase and condensed-phase processes of the boron fire retardant and the condensed-phase process of the clay.
25723711	7	17	theme	flammability	950:961	arg1	reduction					963:971	This flammability reduction	945:971	This flammability reduction	945:971	This flammability reduction was caused by three mechanisms-the gas-phase and condensed-phase processes of the boron fire retardant and the condensed-phase process of the clay.
25723711	8	18	from	process	1153:1159	arg1	flammability					1206:1217	flammability	1206:1217	flammability	1206:1217	We describe the one-pot coating process and the impact of the coating composition on flammability.
25723711	4	19	theme	%	622:622	arg1	reduction					624:632	a 75% reduction	618:632	a 75% reduction in PHRR	618:640	One formulation produced a 75% reduction in PHRR.
25723711	1	20	theme	pot	156:158	arg1	"					159:159	a single "pot"	146:159	a single "pot" to produce flexible, highly fire resistant, and bioinspired coatings on flexible polyurethane foam (PUF)	146:264	In this manuscript, natural materials were combined into a single "pot" to produce flexible, highly fire resistant, and bioinspired coatings on flexible polyurethane foam (PUF).
25723711	7	21	theme	clay	1115:1118	arg1	process					1100:1106	the condensed-phase process	1080:1106	the condensed-phase process of the clay	1080:1118	This flammability reduction was caused by three mechanisms-the gas-phase and condensed-phase processes of the boron fire retardant and the condensed-phase process of the clay.
25723711	7	21	theme	clay	1115:1118	arg1	processes					1038:1046	three mechanisms-the gas-phase and condensed-phase processes	987:1046	three mechanisms-the gas-phase and condensed-phase processes of the boron fire retardant	987:1074	This flammability reduction was caused by three mechanisms-the gas-phase and condensed-phase processes of the boron fire retardant and the condensed-phase process of the clay.
25723711	6	22	from	damage	855:860	arg1	threat					813:818	the fire threat	804:818	the fire threat of furniture from significant fire damage in and beyond the room of fire origin to being contained to the burning furniture	804:942	At these PHRR values, this technology could reduce the fire threat of furniture from significant fire damage in and beyond the room of fire origin to being contained to the burning furniture.
25723711	6	22	from	damage	855:860	arg1	furniture					823:831	furniture	823:831	furniture from significant fire damage in	823:863	At these PHRR values, this technology could reduce the fire threat of furniture from significant fire damage in and beyond the room of fire origin to being contained to the burning furniture.
25723711	8	23	theme	coating	1183:1189	arg1	composition					1191:1201	the coating composition	1179:1201	the coating composition	1179:1201	We describe the one-pot coating process and the impact of the coating composition on flammability.
25723711	2	24	dep	retardant	394:402	arg1	derivative					419:428	derivative	419:428	derivative	419:428	In one step, PUF was coated with a fire protective layer constructed of a polysaccharide binder (starch or agar), a boron fire retardant (boric acid or derivative), and a dirt char former (montmorillonite clay).
25723711	2	24	dep	retardant	394:402	arg1	acid					411:414	boric acid	405:414	boric acid	405:414	In one step, PUF was coated with a fire protective layer constructed of a polysaccharide binder (starch or agar), a boron fire retardant (boric acid or derivative), and a dirt char former (montmorillonite clay).
25723711	0	25	theme	One-pot	0:6	arg1	coatings					21:28	One-pot, bioinspired coatings	0:28	One-pot, bioinspired coatings	0:28	One-pot, bioinspired coatings to reduce the flammability of flexible polyurethane foams.
25723711	1	26	theme	polyurethane	242:253	arg1	PUF					261:263	flexible polyurethane foam (PUF)	233:264	flexible polyurethane foam (PUF)	233:264	In this manuscript, natural materials were combined into a single "pot" to produce flexible, highly fire resistant, and bioinspired coatings on flexible polyurethane foam (PUF).
25723711	6	27	from	threat	813:818	arg1	damage					855:860	significant fire damage	838:860	significant fire damage in	838:863	At these PHRR values, this technology could reduce the fire threat of furniture from significant fire damage in and beyond the room of fire origin to being contained to the burning furniture.
25723711	2	28	theme	protective	307:316	arg1	layer					318:322	a fire protective layer	300:322	a fire protective layer constructed of a polysaccharide binder (starch or agar), a boron fire retardant (boric acid or derivative), and a dirt char former (montmorillonite clay)	300:476	In one step, PUF was coated with a fire protective layer constructed of a polysaccharide binder (starch or agar), a boron fire retardant (boric acid or derivative), and a dirt char former (montmorillonite clay).
25723711	1	29	theme	foam	255:258	arg1	PUF					261:263	flexible polyurethane foam (PUF)	233:264	flexible polyurethane foam (PUF)	233:264	In this manuscript, natural materials were combined into a single "pot" to produce flexible, highly fire resistant, and bioinspired coatings on flexible polyurethane foam (PUF).
25723711	5	30	from	reduction	721:729	arg1	PHRR					734:737	PHRR	734:737	PHRR	734:737	This technology was validated in full-scale furniture fire tests, where a 75% reduction in PHRR was measured.
25723711	5	31	theme	full-scale	676:685	arg1	tests					702:706	full-scale furniture fire tests	676:706	full-scale furniture fire tests	676:706	This technology was validated in full-scale furniture fire tests, where a 75% reduction in PHRR was measured.
25723711	2	32	theme	dirt	438:441	arg1	char					443:446	a dirt char former	436:453	a dirt char former (montmorillonite clay)	436:476	In one step, PUF was coated with a fire protective layer constructed of a polysaccharide binder (starch or agar), a boron fire retardant (boric acid or derivative), and a dirt char former (montmorillonite clay).
25723711	2	32	theme	dirt	438:441	arg1	clay					472:475	montmorillonite clay	456:475	montmorillonite clay	456:475	In one step, PUF was coated with a fire protective layer constructed of a polysaccharide binder (starch or agar), a boron fire retardant (boric acid or derivative), and a dirt char former (montmorillonite clay).
25723711	6	33	theme	origin	893:898	arg1	room					880:883	the room	876:883	the room of fire origin	876:898	At these PHRR values, this technology could reduce the fire threat of furniture from significant fire damage in and beyond the room of fire origin to being contained to the burning furniture.
25723711	1	34	theme	flexible	172:179	arg1	coatings					221:228	flexible, highly fire resistant, and bioinspired coatings	172:228	flexible, highly fire resistant, and bioinspired coatings on flexible polyurethane foam (PUF)	172:264	In this manuscript, natural materials were combined into a single "pot" to produce flexible, highly fire resistant, and bioinspired coatings on flexible polyurethane foam (PUF).
25723711	2	35	theme	fire	389:392	arg1	retardant					394:402	a boron fire retardant	381:402	a boron fire retardant (boric acid or derivative)	381:429	In one step, PUF was coated with a fire protective layer constructed of a polysaccharide binder (starch or agar), a boron fire retardant (boric acid or derivative), and a dirt char former (montmorillonite clay).
25723711	6	36	theme	burning	926:932	arg1	furniture					934:942	the burning furniture	922:942	the burning furniture	922:942	At these PHRR values, this technology could reduce the fire threat of furniture from significant fire damage in and beyond the room of fire origin to being contained to the burning furniture.
25723711	5	37	theme	fire	697:700	arg1	tests					702:706	full-scale furniture fire tests	676:706	full-scale furniture fire tests	676:706	This technology was validated in full-scale furniture fire tests, where a 75% reduction in PHRR was measured.
25723711	2	38	theme	polysaccharide	341:354	arg1	binder					356:361	a polysaccharide binder	339:361	a polysaccharide binder (starch or agar)	339:378	In one step, PUF was coated with a fire protective layer constructed of a polysaccharide binder (starch or agar), a boron fire retardant (boric acid or derivative), and a dirt char former (montmorillonite clay).
25723711	2	39	theme	boron	383:387	arg1	retardant					394:402	a boron fire retardant	381:402	a boron fire retardant (boric acid or derivative)	381:429	In one step, PUF was coated with a fire protective layer constructed of a polysaccharide binder (starch or agar), a boron fire retardant (boric acid or derivative), and a dirt char former (montmorillonite clay).
25723711	1	40	from	coatings	221:228	arg1	PUF					261:263	flexible polyurethane foam (PUF)	233:264	flexible polyurethane foam (PUF)	233:264	In this manuscript, natural materials were combined into a single "pot" to produce flexible, highly fire resistant, and bioinspired coatings on flexible polyurethane foam (PUF).
25723711	3	41	theme	%	512:512	arg1	reduction					514:522	a 63% reduction	508:522	a 63% reduction in a critical flammability value, the peak heat release rate (PHRR)	508:590	Nearly all coatings produced a 63% reduction in a critical flammability value, the peak heat release rate (PHRR).
25723711	5	42	theme	%	719:719	arg1	reduction					721:729	a 75% reduction	715:729	a 75% reduction in PHRR	715:737	This technology was validated in full-scale furniture fire tests, where a 75% reduction in PHRR was measured.
25723711	7	43	theme	boron	1055:1059	arg1	retardant					1066:1074	the boron fire retardant	1051:1074	the boron fire retardant	1051:1074	This flammability reduction was caused by three mechanisms-the gas-phase and condensed-phase processes of the boron fire retardant and the condensed-phase process of the clay.
25723711	6	44	theme	PHRR	762:765	arg1	values					767:772	these PHRR values	756:772	these PHRR values	756:772	At these PHRR values, this technology could reduce the fire threat of furniture from significant fire damage in and beyond the room of fire origin to being contained to the burning furniture.
25723711	2	45	theme	montmorillonite	456:470	arg1	char					443:446	a dirt char former	436:453	a dirt char former (montmorillonite clay)	436:476	In one step, PUF was coated with a fire protective layer constructed of a polysaccharide binder (starch or agar), a boron fire retardant (boric acid or derivative), and a dirt char former (montmorillonite clay).
25723711	2	45	theme	montmorillonite	456:470	arg1	clay					472:475	montmorillonite clay	456:475	montmorillonite clay	456:475	In one step, PUF was coated with a fire protective layer constructed of a polysaccharide binder (starch or agar), a boron fire retardant (boric acid or derivative), and a dirt char former (montmorillonite clay).
25723711	2	46	dep	binder	356:361	arg1	agar					374:377	agar	374:377	agar	374:377	In one step, PUF was coated with a fire protective layer constructed of a polysaccharide binder (starch or agar), a boron fire retardant (boric acid or derivative), and a dirt char former (montmorillonite clay).
25723711	2	46	dep	binder	356:361	arg1	starch					364:369	starch	364:369	starch	364:369	In one step, PUF was coated with a fire protective layer constructed of a polysaccharide binder (starch or agar), a boron fire retardant (boric acid or derivative), and a dirt char former (montmorillonite clay).
25723711	7	47	theme	condensed-phase	1022:1036	arg1	processes					1038:1046	three mechanisms-the gas-phase and condensed-phase processes	987:1046	three mechanisms-the gas-phase and condensed-phase processes of the boron fire retardant	987:1074	This flammability reduction was caused by three mechanisms-the gas-phase and condensed-phase processes of the boron fire retardant and the condensed-phase process of the clay.
25723711	1	48	theme	natural	109:115	arg1	materials					117:125	natural materials	109:125	natural materials	109:125	In this manuscript, natural materials were combined into a single "pot" to produce flexible, highly fire resistant, and bioinspired coatings on flexible polyurethane foam (PUF).
25723711	6	49	theme	significant	838:848	arg1	damage					855:860	significant fire damage	838:860	significant fire damage in	838:863	At these PHRR values, this technology could reduce the fire threat of furniture from significant fire damage in and beyond the room of fire origin to being contained to the burning furniture.
25723711	7	50	theme	condensed-phase	1084:1098	arg1	process					1100:1106	the condensed-phase process	1080:1106	the condensed-phase process of the clay	1080:1118	This flammability reduction was caused by three mechanisms-the gas-phase and condensed-phase processes of the boron fire retardant and the condensed-phase process of the clay.
25723711	8	51	from	impact	1169:1174	arg1	flammability					1206:1217	flammability	1206:1217	flammability	1206:1217	We describe the one-pot coating process and the impact of the coating composition on flammability.
25723711	6	52	theme	fire	850:853	arg1	damage					855:860	significant fire damage	838:860	significant fire damage in	838:863	At these PHRR values, this technology could reduce the fire threat of furniture from significant fire damage in and beyond the room of fire origin to being contained to the burning furniture.
25723711	2	53	theme	former	448:453	arg1	char					443:446	a dirt char former	436:453	a dirt char former (montmorillonite clay)	436:476	In one step, PUF was coated with a fire protective layer constructed of a polysaccharide binder (starch or agar), a boron fire retardant (boric acid or derivative), and a dirt char former (montmorillonite clay).
25723711	2	53	theme	former	448:453	arg1	clay					472:475	montmorillonite clay	456:475	montmorillonite clay	456:475	In one step, PUF was coated with a fire protective layer constructed of a polysaccharide binder (starch or agar), a boron fire retardant (boric acid or derivative), and a dirt char former (montmorillonite clay).
25723711	3	54	theme	critical	529:536	arg1	value					551:555	a critical flammability value	527:555	a critical flammability value	527:555	Nearly all coatings produced a 63% reduction in a critical flammability value, the peak heat release rate (PHRR).
25723711	3	54	theme	critical	529:536	arg1	rate					580:583	the peak heat release rate	558:583	the peak heat release rate (PHRR)	558:590	Nearly all coatings produced a 63% reduction in a critical flammability value, the peak heat release rate (PHRR).
25723711	3	55	theme	63	510:511	arg1	%					512:512	%	512:512	%	512:512	Nearly all coatings produced a 63% reduction in a critical flammability value, the peak heat release rate (PHRR).
25723711	1	56	theme	resistant	194:202	arg1	coatings					221:228	flexible, highly fire resistant, and bioinspired coatings	172:228	flexible, highly fire resistant, and bioinspired coatings on flexible polyurethane foam (PUF)	172:264	In this manuscript, natural materials were combined into a single "pot" to produce flexible, highly fire resistant, and bioinspired coatings on flexible polyurethane foam (PUF).
25723711	0	57	theme	flexible	60:67	arg1	foams					82:86	flexible polyurethane foams	60:86	flexible polyurethane foams	60:86	One-pot, bioinspired coatings to reduce the flammability of flexible polyurethane foams.
25723711	3	58	theme	flammability	538:549	arg1	value					551:555	a critical flammability value	527:555	a critical flammability value	527:555	Nearly all coatings produced a 63% reduction in a critical flammability value, the peak heat release rate (PHRR).
25723711	3	58	theme	flammability	538:549	arg1	rate					580:583	the peak heat release rate	558:583	the peak heat release rate (PHRR)	558:590	Nearly all coatings produced a 63% reduction in a critical flammability value, the peak heat release rate (PHRR).
25723711	2	59	theme	fire	302:305	arg1	layer					318:322	a fire protective layer	300:322	a fire protective layer constructed of a polysaccharide binder (starch or agar), a boron fire retardant (boric acid or derivative), and a dirt char former (montmorillonite clay)	300:476	In one step, PUF was coated with a fire protective layer constructed of a polysaccharide binder (starch or agar), a boron fire retardant (boric acid or derivative), and a dirt char former (montmorillonite clay).
25723711	2	60	theme	boric	405:409	arg1	acid					411:414	boric acid	405:414	boric acid	405:414	In one step, PUF was coated with a fire protective layer constructed of a polysaccharide binder (starch or agar), a boron fire retardant (boric acid or derivative), and a dirt char former (montmorillonite clay).
25723711	3	61	theme	heat	567:570	arg1	value					551:555	a critical flammability value	527:555	a critical flammability value	527:555	Nearly all coatings produced a 63% reduction in a critical flammability value, the peak heat release rate (PHRR).
25723711	3	61	theme	heat	567:570	arg1	PHRR					586:589	PHRR	586:589	PHRR	586:589	Nearly all coatings produced a 63% reduction in a critical flammability value, the peak heat release rate (PHRR).
25723711	3	61	theme	heat	567:570	arg1	rate					580:583	the peak heat release rate	558:583	the peak heat release rate (PHRR)	558:590	Nearly all coatings produced a 63% reduction in a critical flammability value, the peak heat release rate (PHRR).
25723711	0	62	dep	One-pot	0:6	arg1	bioinspired					9:19	bioinspired	9:19	bioinspired	9:19	One-pot, bioinspired coatings to reduce the flammability of flexible polyurethane foams.
25723711	6	63	theme	furniture	823:831	arg1	threat					813:818	the fire threat	804:818	the fire threat of furniture from significant fire damage in and beyond the room of fire origin to being contained to the burning furniture	804:942	At these PHRR values, this technology could reduce the fire threat of furniture from significant fire damage in and beyond the room of fire origin to being contained to the burning furniture.
25498647	5	0	theme	more	862:865	arg1	residues					895:902	the more acid labile arabinofuranose residues	858:902	the more acid labile arabinofuranose residues present on the pectin side chain	858:935	Higher temperatures, lower pHs and longer extraction times lead to a loss of the more acid labile arabinofuranose residues present on the pectin side chain.
25498647	0	1	theme	polysaccharides	75:89	arg1	characterisation					48:63	the chemical characterisation	35:63	the chemical characterisation of pectin polysaccharides	35:89	An experimental design approach to the chemical characterisation of pectin polysaccharides extracted from Cucumis melo Inodorus.
25498647	5	2	attach	present	904:910	arg2	residues					895:902	the more acid labile arabinofuranose residues	858:902	the more acid labile arabinofuranose residues present on the pectin side chain	858:935	Higher temperatures, lower pHs and longer extraction times lead to a loss of the more acid labile arabinofuranose residues present on the pectin side chain.
25498647	5	2	attach	present	904:910	arg1	chain					931:935	the pectin side chain	915:935	the pectin side chain	915:935	Higher temperatures, lower pHs and longer extraction times lead to a loss of the more acid labile arabinofuranose residues present on the pectin side chain.
25498647	5	3	theme	acid	867:870	arg1	residues					895:902	the more acid labile arabinofuranose residues	858:902	the more acid labile arabinofuranose residues present on the pectin side chain	858:935	Higher temperatures, lower pHs and longer extraction times lead to a loss of the more acid labile arabinofuranose residues present on the pectin side chain.
25498647	6	4	theme	equations	964:972	arg1	fitting					942:948	The fitting	938:948	The fitting of regression equations relating yield and composition to extraction conditions	938:1028	The fitting of regression equations relating yield and composition to extraction conditions can therefore lead to tailor-made pectins for specific properties and/or applications.
25498647	1	5	theme	pectins	339:345	arg1	number					329:334	a number	327:334	a number of pectins	327:345	Extracted pectins have been utilised in a number of applications in both the food and pharmaceutical industries where they are generally used as gelling agents, thickeners and stabilisers, although a number of pectins have been shown to be bioactive.
25498647	1	5	theme	pectins	339:345	arg1	bioactive					369:377	bioactive	369:377	bioactive	369:377	Extracted pectins have been utilised in a number of applications in both the food and pharmaceutical industries where they are generally used as gelling agents, thickeners and stabilisers, although a number of pectins have been shown to be bioactive.
25498647	6	6	theme	regression	953:962	arg1	equations					964:972	regression equations	953:972	regression equations relating yield and composition to extraction conditions	953:1028	The fitting of regression equations relating yield and composition to extraction conditions can therefore lead to tailor-made pectins for specific properties and/or applications.
25498647	3	7	from	effects	513:519	arg1	pectins					574:580	pectins	574:580	pectins extracted from Cucumis melo Inodorus	574:617	A statistical experimental design approach was used to study the effects of extraction conditions pH, time and temperature on pectins extracted from Cucumis melo Inodorus.
25498647	6	8	theme	specific	1076:1083	arg1	properties					1085:1094	specific properties	1076:1094	specific properties	1076:1094	The fitting of regression equations relating yield and composition to extraction conditions can therefore lead to tailor-made pectins for specific properties and/or applications.
25498647	5	9	theme	extraction	823:832	arg1	times					834:838	longer extraction times	816:838	longer extraction times	816:838	Higher temperatures, lower pHs and longer extraction times lead to a loss of the more acid labile arabinofuranose residues present on the pectin side chain.
25498647	4	10	contain	has	719:721	arg1	this					714:717	this	714:717	this	714:717	The results show that the chemical composition is very sensitive to these conditions and that this has a great influence on for example the degree of branching.
25498647	4	10	contain	has	719:721	arg2	influence					731:739	a great influence	723:739	a great influence	723:739	The results show that the chemical composition is very sensitive to these conditions and that this has a great influence on for example the degree of branching.
25498647	5	11	theme	longer	816:821	arg1	times					834:838	longer extraction times	816:838	longer extraction times	816:838	Higher temperatures, lower pHs and longer extraction times lead to a loss of the more acid labile arabinofuranose residues present on the pectin side chain.
25498647	3	12	theme	extraction	524:533	arg1	pH					546:547	pH	546:547	pH	546:547	A statistical experimental design approach was used to study the effects of extraction conditions pH, time and temperature on pectins extracted from Cucumis melo Inodorus.
25498647	3	12	theme	extraction	524:533	arg1	temperature					559:569	temperature	559:569	temperature	559:569	A statistical experimental design approach was used to study the effects of extraction conditions pH, time and temperature on pectins extracted from Cucumis melo Inodorus.
25498647	3	12	theme	extraction	524:533	arg1	conditions					535:544	extraction conditions pH, time and temperature	524:569	extraction conditions pH, time and temperature	524:569	A statistical experimental design approach was used to study the effects of extraction conditions pH, time and temperature on pectins extracted from Cucumis melo Inodorus.
25498647	3	12	theme	extraction	524:533	arg1	time					550:553	time	550:553	time	550:553	A statistical experimental design approach was used to study the effects of extraction conditions pH, time and temperature on pectins extracted from Cucumis melo Inodorus.
25498647	5	13	theme	Higher	781:786	arg1	temperatures					788:799	Higher temperatures	781:799	Higher temperatures	781:799	Higher temperatures, lower pHs and longer extraction times lead to a loss of the more acid labile arabinofuranose residues present on the pectin side chain.
25498647	3	14	theme	conditions	535:544	arg1	effects					513:519	the effects	509:519	the effects of extraction conditions pH, time and temperature on pectins extracted from Cucumis melo Inodorus	509:617	A statistical experimental design approach was used to study the effects of extraction conditions pH, time and temperature on pectins extracted from Cucumis melo Inodorus.
25498647	4	15	theme	chemical	646:653	arg1	composition					655:665	the chemical composition	642:665	the chemical composition	642:665	The results show that the chemical composition is very sensitive to these conditions and that this has a great influence on for example the degree of branching.
25498647	4	15	theme	chemical	646:653	arg1	sensitive					675:683	sensitive	675:683	sensitive	675:683	The results show that the chemical composition is very sensitive to these conditions and that this has a great influence on for example the degree of branching.
25498647	0	16	theme	experimental	3:14	arg1	approach					23:30	An experimental design approach	0:30	An experimental design approach to the chemical characterisation of pectin polysaccharides	0:89	An experimental design approach to the chemical characterisation of pectin polysaccharides extracted from Cucumis melo Inodorus.
25498647	4	17	theme	great	725:729	arg1	influence					731:739	a great influence	723:739	a great influence	723:739	The results show that the chemical composition is very sensitive to these conditions and that this has a great influence on for example the degree of branching.
25498647	1	18	theme	applications	181:192	arg1	number					171:176	a number	169:176	a number of applications in both the food and pharmaceutical industries where they are generally used as gelling agents, thickeners and stabilisers, although a number of pectins have been shown to be bioactive	169:377	Extracted pectins have been utilised in a number of applications in both the food and pharmaceutical industries where they are generally used as gelling agents, thickeners and stabilisers, although a number of pectins have been shown to be bioactive.
25498647	1	19	theme	gelling	274:280	arg1	they					247:250	they	247:250	they	247:250	Extracted pectins have been utilised in a number of applications in both the food and pharmaceutical industries where they are generally used as gelling agents, thickeners and stabilisers, although a number of pectins have been shown to be bioactive.
25498647	1	19	theme	gelling	274:280	arg1	agents					282:287	gelling agents	274:287	gelling agents	274:287	Extracted pectins have been utilised in a number of applications in both the food and pharmaceutical industries where they are generally used as gelling agents, thickeners and stabilisers, although a number of pectins have been shown to be bioactive.
25498647	2	20	theme	extraction	425:434	arg1	conditions					436:445	extraction conditions	425:445	extraction conditions	425:445	These functional properties will depend upon extraction conditions.
25498647	5	21	theme	pectin	919:924	arg1	chain					931:935	the pectin side chain	915:935	the pectin side chain	915:935	Higher temperatures, lower pHs and longer extraction times lead to a loss of the more acid labile arabinofuranose residues present on the pectin side chain.
25498647	3	22	theme	experimental	462:473	arg1	approach					482:489	A statistical experimental design approach	448:489	A statistical experimental design approach	448:489	A statistical experimental design approach was used to study the effects of extraction conditions pH, time and temperature on pectins extracted from Cucumis melo Inodorus.
25498647	0	23	theme	design	16:21	arg1	approach					23:30	An experimental design approach	0:30	An experimental design approach to the chemical characterisation of pectin polysaccharides	0:89	An experimental design approach to the chemical characterisation of pectin polysaccharides extracted from Cucumis melo Inodorus.
25498647	5	24	theme	side	926:929	arg1	chain					931:935	the pectin side chain	915:935	the pectin side chain	915:935	Higher temperatures, lower pHs and longer extraction times lead to a loss of the more acid labile arabinofuranose residues present on the pectin side chain.
25498647	3	25	theme	design	475:480	arg1	approach					482:489	A statistical experimental design approach	448:489	A statistical experimental design approach	448:489	A statistical experimental design approach was used to study the effects of extraction conditions pH, time and temperature on pectins extracted from Cucumis melo Inodorus.
25498647	3	26	dep	conditions	535:544	arg1	pH					546:547	pH	546:547	pH	546:547	A statistical experimental design approach was used to study the effects of extraction conditions pH, time and temperature on pectins extracted from Cucumis melo Inodorus.
25498647	3	26	dep	conditions	535:544	arg1	temperature					559:569	temperature	559:569	temperature	559:569	A statistical experimental design approach was used to study the effects of extraction conditions pH, time and temperature on pectins extracted from Cucumis melo Inodorus.
25498647	3	26	dep	conditions	535:544	arg1	conditions					535:544	extraction conditions pH, time and temperature	524:569	extraction conditions pH, time and temperature	524:569	A statistical experimental design approach was used to study the effects of extraction conditions pH, time and temperature on pectins extracted from Cucumis melo Inodorus.
25498647	3	26	dep	conditions	535:544	arg1	time					550:553	time	550:553	time	550:553	A statistical experimental design approach was used to study the effects of extraction conditions pH, time and temperature on pectins extracted from Cucumis melo Inodorus.
25498647	1	27	used	used	266:269	arg2	thickeners					290:299	thickeners	290:299	thickeners	290:299	Extracted pectins have been utilised in a number of applications in both the food and pharmaceutical industries where they are generally used as gelling agents, thickeners and stabilisers, although a number of pectins have been shown to be bioactive.
25498647	1	27	used	used	266:269	arg2	stabilisers					305:315	stabilisers	305:315	stabilisers	305:315	Extracted pectins have been utilised in a number of applications in both the food and pharmaceutical industries where they are generally used as gelling agents, thickeners and stabilisers, although a number of pectins have been shown to be bioactive.
25498647	1	27	used	used	266:269	arg2	they					247:250	they	247:250	they	247:250	Extracted pectins have been utilised in a number of applications in both the food and pharmaceutical industries where they are generally used as gelling agents, thickeners and stabilisers, although a number of pectins have been shown to be bioactive.
25498647	1	27	used	used	266:269	arg2	agents					282:287	gelling agents	274:287	gelling agents	274:287	Extracted pectins have been utilised in a number of applications in both the food and pharmaceutical industries where they are generally used as gelling agents, thickeners and stabilisers, although a number of pectins have been shown to be bioactive.
25498647	1	28	theme	food	206:209	arg1	industries					230:239	both the food and pharmaceutical industries	197:239	industries	230:239	Extracted pectins have been utilised in a number of applications in both the food and pharmaceutical industries where they are generally used as gelling agents, thickeners and stabilisers, although a number of pectins have been shown to be bioactive.
25498647	5	29	theme	arabinofuranose	879:893	arg1	residues					895:902	the more acid labile arabinofuranose residues	858:902	the more acid labile arabinofuranose residues present on the pectin side chain	858:935	Higher temperatures, lower pHs and longer extraction times lead to a loss of the more acid labile arabinofuranose residues present on the pectin side chain.
25498647	2	30	theme	functional	386:395	arg1	properties					397:406	These functional properties	380:406	These functional properties	380:406	These functional properties will depend upon extraction conditions.
25498647	6	31	theme	extraction	1008:1017	arg1	conditions					1019:1028	extraction conditions	1008:1028	extraction conditions	1008:1028	The fitting of regression equations relating yield and composition to extraction conditions can therefore lead to tailor-made pectins for specific properties and/or applications.
25498647	1	32	dep	industries	230:239	arg1	both					197:200	both	197:200	both	197:200	Extracted pectins have been utilised in a number of applications in both the food and pharmaceutical industries where they are generally used as gelling agents, thickeners and stabilisers, although a number of pectins have been shown to be bioactive.
25498647	5	33	theme	lower	802:806	arg1	pHs					808:810	lower pHs	802:810	lower pHs	802:810	Higher temperatures, lower pHs and longer extraction times lead to a loss of the more acid labile arabinofuranose residues present on the pectin side chain.
25498647	5	34	theme	labile	872:877	arg1	residues					895:902	the more acid labile arabinofuranose residues	858:902	the more acid labile arabinofuranose residues present on the pectin side chain	858:935	Higher temperatures, lower pHs and longer extraction times lead to a loss of the more acid labile arabinofuranose residues present on the pectin side chain.
25498647	0	35	theme	chemical	39:46	arg1	characterisation					48:63	the chemical characterisation	35:63	the chemical characterisation of pectin polysaccharides	35:89	An experimental design approach to the chemical characterisation of pectin polysaccharides extracted from Cucumis melo Inodorus.
25498647	3	36	used	used	495:498	arg2	approach					482:489	A statistical experimental design approach	448:489	A statistical experimental design approach	448:489	A statistical experimental design approach was used to study the effects of extraction conditions pH, time and temperature on pectins extracted from Cucumis melo Inodorus.
25498647	1	37	theme	Extracted	129:137	arg1	pectins					139:145	Extracted pectins	129:145	Extracted pectins	129:145	Extracted pectins have been utilised in a number of applications in both the food and pharmaceutical industries where they are generally used as gelling agents, thickeners and stabilisers, although a number of pectins have been shown to be bioactive.
25498647	4	38	theme	branching	770:778	arg1	degree					760:765	the degree	756:765	for example the degree of branching	744:778	The results show that the chemical composition is very sensitive to these conditions and that this has a great influence on for example the degree of branching.
25498647	5	39	theme	residues	895:902	arg1	loss					850:853	a loss	848:853	a loss of the more acid labile arabinofuranose residues present on the pectin side chain	848:935	Higher temperatures, lower pHs and longer extraction times lead to a loss of the more acid labile arabinofuranose residues present on the pectin side chain.
25498647	1	40	theme	pharmaceutical	215:228	arg1	industries					230:239	both the food and pharmaceutical industries	197:239	industries	230:239	Extracted pectins have been utilised in a number of applications in both the food and pharmaceutical industries where they are generally used as gelling agents, thickeners and stabilisers, although a number of pectins have been shown to be bioactive.
25498647	1	41	from	industries	230:239	arg1	number					171:176	a number	169:176	a number of applications in both the food and pharmaceutical industries where they are generally used as gelling agents, thickeners and stabilisers, although a number of pectins have been shown to be bioactive	169:377	Extracted pectins have been utilised in a number of applications in both the food and pharmaceutical industries where they are generally used as gelling agents, thickeners and stabilisers, although a number of pectins have been shown to be bioactive.
25498647	1	42	from	applications	181:192	arg1	industries					230:239	both the food and pharmaceutical industries	197:239	industries	230:239	Extracted pectins have been utilised in a number of applications in both the food and pharmaceutical industries where they are generally used as gelling agents, thickeners and stabilisers, although a number of pectins have been shown to be bioactive.
25498647	5	43	theme	present	904:910	arg1	residues					895:902	the more acid labile arabinofuranose residues	858:902	the more acid labile arabinofuranose residues present on the pectin side chain	858:935	Higher temperatures, lower pHs and longer extraction times lead to a loss of the more acid labile arabinofuranose residues present on the pectin side chain.
25498647	0	44	theme	pectin	68:73	arg1	polysaccharides					75:89	pectin polysaccharides	68:89	pectin polysaccharides	68:89	An experimental design approach to the chemical characterisation of pectin polysaccharides extracted from Cucumis melo Inodorus.
25498647	3	45	theme	statistical	450:460	arg1	approach					482:489	A statistical experimental design approach	448:489	A statistical experimental design approach	448:489	A statistical experimental design approach was used to study the effects of extraction conditions pH, time and temperature on pectins extracted from Cucumis melo Inodorus.
25498647	6	46	theme	tailor-made	1052:1062	arg1	pectins					1064:1070	tailor-made pectins	1052:1070	tailor-made pectins for specific properties and/or applications	1052:1114	The fitting of regression equations relating yield and composition to extraction conditions can therefore lead to tailor-made pectins for specific properties and/or applications.
25498647	1	47	from	number	171:176	arg1	industries					230:239	both the food and pharmaceutical industries	197:239	industries	230:239	Extracted pectins have been utilised in a number of applications in both the food and pharmaceutical industries where they are generally used as gelling agents, thickeners and stabilisers, although a number of pectins have been shown to be bioactive.
26161688	11	0	theme	concomitant	2139:2149	arg1	migration					2173:2181	concomitant single and collective migration	2139:2181	concomitant single and collective migration as a function of the microenvironment topography to reach the most productive migration strategy	2139:2278	Moreover, GSC neurospheres exhibit concomitant single and collective migration as a function of the microenvironment topography to reach the most productive migration strategy.
26161688	9	1	theme	3D	1889:1890	arg1	matrix					1892:1897	the 3D matrix	1885:1897	the 3D matrix	1885:1897	In the dual niche system, the presence of HA reduced velocity and number of migratory cells; however, cells that came in contact with the pseudovessels exhibited collective migration by an extensive strand and reached higher velocities than cells migrating individually across the 3D matrix.
26161688	3	2	theme	composite	514:522	arg1	matrix					524:529	a composite matrix	512:529	a composite matrix of hyaluronan (HA) structurally supported by a collagen-oligomer fibril network to simulate the brain tumor extracellular matrix (ECM) composition	512:676	In this study, we developed a composite matrix of hyaluronan (HA) structurally supported by a collagen-oligomer fibril network to simulate the brain tumor extracellular matrix (ECM) composition.
26161688	10	3	theme	migration	1940:1948	arg1	mechanisms					1950:1959	varied migration mechanisms	1933:1959	varied migration mechanisms	1933:1959	Our results show that GSCs adopt varied migration mechanisms to invade multiple ECM microenvironments, and the migration characteristics exhibited are highly influenced by the matrix physical properties.
26161688	2	4	theme	glioblastoma	300:311	arg1	GSCs					325:328	GSCs	325:328	GSCs	325:328	A subpopulation termed glioblastoma stem cells (GSCs) has been directly related to aggressive invasion; nonetheless, their migratory characteristics and regulation by the microenvironment are still unknown.
26161688	2	4	theme	glioblastoma	300:311	arg1	cells					318:322	glioblastoma stem cells	300:322	glioblastoma stem cells (GSCs)	300:329	A subpopulation termed glioblastoma stem cells (GSCs) has been directly related to aggressive invasion; nonetheless, their migratory characteristics and regulation by the microenvironment are still unknown.
26161688	7	5	theme	single-cell	1455:1465	arg1	migration					1467:1475	mesenchymal single-cell migration	1443:1475	mesenchymal single-cell migration in collagen matrices	1443:1496	The cells altered their migration mode depending on the matrix composition, showing migration by expansive growth in Matrigel matrices, multicellular extension along rigid interfaces (as Matrigel glass and coated microfibers), and mesenchymal single-cell migration in collagen matrices.
26161688	4	6	theme	Matrigel-coated	679:693	arg1	microfibers					695:705	Matrigel-coated microfibers	679:705	Matrigel-coated microfibers	679:705	Matrigel-coated microfibers were embedded within the matrix to create a tunable dual niche microenvironment that resembles the vascular network of the brain.
26161688	10	7	theme	multiple	1971:1978	arg1	microenvironments					1984:2000	multiple ECM microenvironments	1971:2000	multiple ECM microenvironments	1971:2000	Our results show that GSCs adopt varied migration mechanisms to invade multiple ECM microenvironments, and the migration characteristics exhibited are highly influenced by the matrix physical properties.
26161688	1	8	theme	main	200:203	arg1	cause					205:209	the main cause	196:209	the main cause of unsuccessful total resection that leads to tumor reappearance	196:274	Diffuse infiltration across brain tissue is a hallmark of glioblastoma and the main cause of unsuccessful total resection that leads to tumor reappearance.
26161688	9	9	theme	cells	1694:1698	arg1	number					1674:1679	number	1674:1679	number	1674:1679	In the dual niche system, the presence of HA reduced velocity and number of migratory cells; however, cells that came in contact with the pseudovessels exhibited collective migration by an extensive strand and reached higher velocities than cells migrating individually across the 3D matrix.
26161688	9	9	theme	cells	1694:1698	arg1	velocity					1661:1668	velocity	1661:1668	velocity	1661:1668	In the dual niche system, the presence of HA reduced velocity and number of migratory cells; however, cells that came in contact with the pseudovessels exhibited collective migration by an extensive strand and reached higher velocities than cells migrating individually across the 3D matrix.
26161688	5	10	theme	migration	1063:1071	arg1	characteristics					1073:1087	the migration characteristics	1059:1087	the migration characteristics of GSC neurospheres	1059:1107	This model was compared with the most commonly used in vitro three-dimensional (3D) culture formats, Matrigel and collagen type-I monomer matrices, to study how the mechanical and compositional properties of the ECM alter the migration characteristics of GSC neurospheres.
26161688	7	11	from	migration	1467:1475	arg1	matrices					1489:1496	collagen matrices	1480:1496	collagen matrices	1480:1496	The cells altered their migration mode depending on the matrix composition, showing migration by expansive growth in Matrigel matrices, multicellular extension along rigid interfaces (as Matrigel glass and coated microfibers), and mesenchymal single-cell migration in collagen matrices.
26161688	7	12	theme	rigid	1378:1382	arg1	interfaces					1384:1393	rigid interfaces	1378:1393	rigid interfaces (as Matrigel glass and coated microfibers)	1378:1436	The cells altered their migration mode depending on the matrix composition, showing migration by expansive growth in Matrigel matrices, multicellular extension along rigid interfaces (as Matrigel glass and coated microfibers), and mesenchymal single-cell migration in collagen matrices.
26161688	1	13	theme	total	227:231	arg1	resection					233:241	unsuccessful total resection	214:241	unsuccessful total resection that leads to tumor reappearance	214:274	Diffuse infiltration across brain tissue is a hallmark of glioblastoma and the main cause of unsuccessful total resection that leads to tumor reappearance.
26161688	3	14	theme	fibril	596:601	arg1	network					603:609	a collagen-oligomer fibril network	576:609	a collagen-oligomer fibril network to simulate the brain tumor extracellular matrix (ECM) composition	576:676	In this study, we developed a composite matrix of hyaluronan (HA) structurally supported by a collagen-oligomer fibril network to simulate the brain tumor extracellular matrix (ECM) composition.
26161688	5	15	theme	culture	921:927	arg1	formats					929:935	the most commonly used in vitro three-dimensional (3D) culture formats	866:935	the most commonly used in vitro three-dimensional (3D) culture formats	866:935	This model was compared with the most commonly used in vitro three-dimensional (3D) culture formats, Matrigel and collagen type-I monomer matrices, to study how the mechanical and compositional properties of the ECM alter the migration characteristics of GSC neurospheres.
26161688	6	16	theme	72-h	1199:1202	arg1	period					1204:1209	a 72-h period	1197:1209	a 72-h period	1197:1209	The migration mode, distance, velocity, and morphology of the GSCs were monitored over a 72-h period.
26161688	3	17	theme	tumor	633:637	arg1	ECM					661:663	ECM	661:663	ECM	661:663	In this study, we developed a composite matrix of hyaluronan (HA) structurally supported by a collagen-oligomer fibril network to simulate the brain tumor extracellular matrix (ECM) composition.
26161688	3	17	theme	tumor	633:637	arg1	matrix					653:658	the brain tumor extracellular matrix	623:658	the brain tumor extracellular matrix (ECM) composition	623:676	In this study, we developed a composite matrix of hyaluronan (HA) structurally supported by a collagen-oligomer fibril network to simulate the brain tumor extracellular matrix (ECM) composition.
26161688	11	18	theme	productive	2250:2259	arg1	strategy					2271:2278	the most productive migration strategy	2241:2278	the most productive migration strategy	2241:2278	Moreover, GSC neurospheres exhibit concomitant single and collective migration as a function of the microenvironment topography to reach the most productive migration strategy.
26161688	7	19	theme	Matrigel	1329:1336	arg1	matrices					1338:1345	Matrigel matrices	1329:1345	Matrigel matrices	1329:1345	The cells altered their migration mode depending on the matrix composition, showing migration by expansive growth in Matrigel matrices, multicellular extension along rigid interfaces (as Matrigel glass and coated microfibers), and mesenchymal single-cell migration in collagen matrices.
26161688	10	20	theme	migration	2011:2019	arg1	characteristics					2021:2035	the migration characteristics	2007:2035	the migration characteristics exhibited are highly influenced by the matrix physical properties	2007:2101	Our results show that GSCs adopt varied migration mechanisms to invade multiple ECM microenvironments, and the migration characteristics exhibited are highly influenced by the matrix physical properties.
26161688	0	21	theme	Glioblastoma	67:78	arg1	Cells					85:89	Glioblastoma Stem Cells	67:89	Glioblastoma Stem Cells	67:89	Extracellular Matrix Properties Regulate the Migratory Response of Glioblastoma Stem Cells in Three-Dimensional Culture.
26161688	5	22	theme	three-dimensional	898:914	arg1	formats					929:935	the most commonly used in vitro three-dimensional (3D) culture formats	866:935	the most commonly used in vitro three-dimensional (3D) culture formats	866:935	This model was compared with the most commonly used in vitro three-dimensional (3D) culture formats, Matrigel and collagen type-I monomer matrices, to study how the mechanical and compositional properties of the ECM alter the migration characteristics of GSC neurospheres.
26161688	0	23	theme	Cells	85:89	arg1	Response					55:62	the Migratory Response	41:62	the Migratory Response of Glioblastoma Stem Cells in Three-Dimensional Culture	41:118	Extracellular Matrix Properties Regulate the Migratory Response of Glioblastoma Stem Cells in Three-Dimensional Culture.
26161688	4	24	theme	dual	759:762	arg1	microenvironment					770:785	a tunable dual niche microenvironment	749:785	a tunable dual niche microenvironment that resembles the vascular network of the brain	749:834	Matrigel-coated microfibers were embedded within the matrix to create a tunable dual niche microenvironment that resembles the vascular network of the brain.
26161688	0	25	theme	Three-Dimensional	94:110	arg1	Culture					112:118	Three-Dimensional Culture	94:118	Three-Dimensional Culture	94:118	Extracellular Matrix Properties Regulate the Migratory Response of Glioblastoma Stem Cells in Three-Dimensional Culture.
26161688	5	26	theme	neurospheres	1096:1107	arg1	characteristics					1073:1087	the migration characteristics	1059:1087	the migration characteristics of GSC neurospheres	1059:1107	This model was compared with the most commonly used in vitro three-dimensional (3D) culture formats, Matrigel and collagen type-I monomer matrices, to study how the mechanical and compositional properties of the ECM alter the migration characteristics of GSC neurospheres.
26161688	0	27	from	Response	55:62	arg1	Culture					112:118	Three-Dimensional Culture	94:118	Three-Dimensional Culture	94:118	Extracellular Matrix Properties Regulate the Migratory Response of Glioblastoma Stem Cells in Three-Dimensional Culture.
26161688	2	28	theme	migratory	400:408	arg1	characteristics					410:424	their migratory characteristics	394:424	their migratory characteristics	394:424	A subpopulation termed glioblastoma stem cells (GSCs) has been directly related to aggressive invasion; nonetheless, their migratory characteristics and regulation by the microenvironment are still unknown.
26161688	4	29	theme	tunable	751:757	arg1	microenvironment					770:785	a tunable dual niche microenvironment	749:785	a tunable dual niche microenvironment that resembles the vascular network of the brain	749:834	Matrigel-coated microfibers were embedded within the matrix to create a tunable dual niche microenvironment that resembles the vascular network of the brain.
26161688	0	30	theme	Extracellular	0:12	arg1	Properties					21:30	Extracellular Matrix Properties	0:30	Extracellular Matrix Properties	0:30	Extracellular Matrix Properties Regulate the Migratory Response of Glioblastoma Stem Cells in Three-Dimensional Culture.
26161688	7	31	from	extension	1362:1370	arg1	matrices					1489:1496	collagen matrices	1480:1496	collagen matrices	1480:1496	The cells altered their migration mode depending on the matrix composition, showing migration by expansive growth in Matrigel matrices, multicellular extension along rigid interfaces (as Matrigel glass and coated microfibers), and mesenchymal single-cell migration in collagen matrices.
26161688	7	32	theme	expansive	1309:1317	arg1	growth					1319:1324	expansive growth	1309:1324	expansive growth in Matrigel matrices	1309:1345	The cells altered their migration mode depending on the matrix composition, showing migration by expansive growth in Matrigel matrices, multicellular extension along rigid interfaces (as Matrigel glass and coated microfibers), and mesenchymal single-cell migration in collagen matrices.
26161688	4	33	theme	niche	764:768	arg1	microenvironment					770:785	a tunable dual niche microenvironment	749:785	a tunable dual niche microenvironment that resembles the vascular network of the brain	749:834	Matrigel-coated microfibers were embedded within the matrix to create a tunable dual niche microenvironment that resembles the vascular network of the brain.
26161688	1	34	theme	Diffuse	121:127	arg1	infiltration					129:140	Diffuse infiltration	121:140	Diffuse infiltration across brain tissue	121:160	Diffuse infiltration across brain tissue is a hallmark of glioblastoma and the main cause of unsuccessful total resection that leads to tumor reappearance.
26161688	1	34	theme	Diffuse	121:127	arg1	hallmark					167:174	a hallmark	165:174	a hallmark of glioblastoma	165:190	Diffuse infiltration across brain tissue is a hallmark of glioblastoma and the main cause of unsuccessful total resection that leads to tumor reappearance.
26161688	5	35	theme	compositional	1017:1029	arg1	properties					1031:1040	the mechanical and compositional properties	998:1040	the mechanical and compositional properties of the ECM	998:1051	This model was compared with the most commonly used in vitro three-dimensional (3D) culture formats, Matrigel and collagen type-I monomer matrices, to study how the mechanical and compositional properties of the ECM alter the migration characteristics of GSC neurospheres.
26161688	11	36	theme	microenvironment	2204:2219	arg1	topography					2221:2230	the microenvironment topography	2200:2230	the microenvironment topography	2200:2230	Moreover, GSC neurospheres exhibit concomitant single and collective migration as a function of the microenvironment topography to reach the most productive migration strategy.
26161688	8	37	theme	matrix	1578:1583	arg1	properties					1596:1605	matrix mechanical properties	1578:1605	matrix mechanical properties	1578:1605	Velocity and distance of migration within each composition varied according to matrix mechanical properties.
26161688	3	38	theme	matrix	653:658	arg1	composition					666:676	the brain tumor extracellular matrix (ECM) composition	623:676	the brain tumor extracellular matrix (ECM) composition	623:676	In this study, we developed a composite matrix of hyaluronan (HA) structurally supported by a collagen-oligomer fibril network to simulate the brain tumor extracellular matrix (ECM) composition.
26161688	11	39	theme	collective	2162:2171	arg1	migration					2173:2181	concomitant single and collective migration	2139:2181	concomitant single and collective migration as a function of the microenvironment topography to reach the most productive migration strategy	2139:2278	Moreover, GSC neurospheres exhibit concomitant single and collective migration as a function of the microenvironment topography to reach the most productive migration strategy.
26161688	9	40	theme	niche	1620:1624	arg1	system					1626:1631	the dual niche system	1611:1631	the dual niche system	1611:1631	In the dual niche system, the presence of HA reduced velocity and number of migratory cells; however, cells that came in contact with the pseudovessels exhibited collective migration by an extensive strand and reached higher velocities than cells migrating individually across the 3D matrix.
26161688	7	41	theme	matrix	1268:1273	arg1	composition					1275:1285	the matrix composition	1264:1285	the matrix composition	1264:1285	The cells altered their migration mode depending on the matrix composition, showing migration by expansive growth in Matrigel matrices, multicellular extension along rigid interfaces (as Matrigel glass and coated microfibers), and mesenchymal single-cell migration in collagen matrices.
26161688	1	42	theme	resection	233:241	arg1	infiltration					129:140	Diffuse infiltration	121:140	Diffuse infiltration across brain tissue	121:160	Diffuse infiltration across brain tissue is a hallmark of glioblastoma and the main cause of unsuccessful total resection that leads to tumor reappearance.
26161688	1	42	theme	resection	233:241	arg1	hallmark					167:174	a hallmark	165:174	a hallmark of glioblastoma	165:190	Diffuse infiltration across brain tissue is a hallmark of glioblastoma and the main cause of unsuccessful total resection that leads to tumor reappearance.
26161688	1	42	theme	resection	233:241	arg1	cause					205:209	the main cause	196:209	the main cause of unsuccessful total resection that leads to tumor reappearance	196:274	Diffuse infiltration across brain tissue is a hallmark of glioblastoma and the main cause of unsuccessful total resection that leads to tumor reappearance.
26161688	10	43	theme	matrix	2076:2081	arg1	properties					2092:2101	the matrix physical properties	2072:2101	the matrix physical properties	2072:2101	Our results show that GSCs adopt varied migration mechanisms to invade multiple ECM microenvironments, and the migration characteristics exhibited are highly influenced by the matrix physical properties.
26161688	7	44	theme	collagen	1480:1487	arg1	matrices					1489:1496	collagen matrices	1480:1496	collagen matrices	1480:1496	The cells altered their migration mode depending on the matrix composition, showing migration by expansive growth in Matrigel matrices, multicellular extension along rigid interfaces (as Matrigel glass and coated microfibers), and mesenchymal single-cell migration in collagen matrices.
26161688	5	45	theme	mechanical	1002:1011	arg1	properties					1031:1040	the mechanical and compositional properties	998:1040	the mechanical and compositional properties of the ECM	998:1051	This model was compared with the most commonly used in vitro three-dimensional (3D) culture formats, Matrigel and collagen type-I monomer matrices, to study how the mechanical and compositional properties of the ECM alter the migration characteristics of GSC neurospheres.
26161688	7	46	from	growth	1319:1324	arg1	matrices					1338:1345	Matrigel matrices	1329:1345	Matrigel matrices	1329:1345	The cells altered their migration mode depending on the matrix composition, showing migration by expansive growth in Matrigel matrices, multicellular extension along rigid interfaces (as Matrigel glass and coated microfibers), and mesenchymal single-cell migration in collagen matrices.
26161688	2	47	theme	aggressive	360:369	arg1	invasion					371:378	aggressive invasion	360:378	aggressive invasion	360:378	A subpopulation termed glioblastoma stem cells (GSCs) has been directly related to aggressive invasion; nonetheless, their migratory characteristics and regulation by the microenvironment are still unknown.
26161688	1	48	theme	tumor	257:261	arg1	reappearance					263:274	tumor reappearance	257:274	tumor reappearance	257:274	Diffuse infiltration across brain tissue is a hallmark of glioblastoma and the main cause of unsuccessful total resection that leads to tumor reappearance.
26161688	9	49	theme	HA	1650:1651	arg1	presence					1638:1645	the presence	1634:1645	the presence of HA	1634:1651	In the dual niche system, the presence of HA reduced velocity and number of migratory cells; however, cells that came in contact with the pseudovessels exhibited collective migration by an extensive strand and reached higher velocities than cells migrating individually across the 3D matrix.
26161688	2	50	theme	stem	313:316	arg1	GSCs					325:328	GSCs	325:328	GSCs	325:328	A subpopulation termed glioblastoma stem cells (GSCs) has been directly related to aggressive invasion; nonetheless, their migratory characteristics and regulation by the microenvironment are still unknown.
26161688	2	50	theme	stem	313:316	arg1	cells					318:322	glioblastoma stem cells	300:322	glioblastoma stem cells (GSCs)	300:329	A subpopulation termed glioblastoma stem cells (GSCs) has been directly related to aggressive invasion; nonetheless, their migratory characteristics and regulation by the microenvironment are still unknown.
26161688	7	51	theme	mesenchymal	1443:1453	arg1	migration					1467:1475	mesenchymal single-cell migration	1443:1475	mesenchymal single-cell migration in collagen matrices	1443:1496	The cells altered their migration mode depending on the matrix composition, showing migration by expansive growth in Matrigel matrices, multicellular extension along rigid interfaces (as Matrigel glass and coated microfibers), and mesenchymal single-cell migration in collagen matrices.
26161688	6	52	theme	GSCs	1172:1175	arg1	velocity					1140:1147	velocity	1140:1147	velocity	1140:1147	The migration mode, distance, velocity, and morphology of the GSCs were monitored over a 72-h period.
26161688	6	52	theme	GSCs	1172:1175	arg1	morphology					1154:1163	morphology	1154:1163	morphology	1154:1163	The migration mode, distance, velocity, and morphology of the GSCs were monitored over a 72-h period.
26161688	6	52	theme	GSCs	1172:1175	arg1	distance					1130:1137	distance	1130:1137	distance	1130:1137	The migration mode, distance, velocity, and morphology of the GSCs were monitored over a 72-h period.
26161688	6	52	theme	GSCs	1172:1175	arg1	mode					1124:1127	The migration mode	1110:1127	The migration mode	1110:1127	The migration mode, distance, velocity, and morphology of the GSCs were monitored over a 72-h period.
26161688	10	53	theme	varied	1933:1938	arg1	mechanisms					1950:1959	varied migration mechanisms	1933:1959	varied migration mechanisms	1933:1959	Our results show that GSCs adopt varied migration mechanisms to invade multiple ECM microenvironments, and the migration characteristics exhibited are highly influenced by the matrix physical properties.
26161688	3	54	theme	hyaluronan	534:543	arg1	matrix					524:529	a composite matrix	512:529	a composite matrix of hyaluronan (HA) structurally supported by a collagen-oligomer fibril network to simulate the brain tumor extracellular matrix (ECM) composition	512:676	In this study, we developed a composite matrix of hyaluronan (HA) structurally supported by a collagen-oligomer fibril network to simulate the brain tumor extracellular matrix (ECM) composition.
26161688	1	55	theme	glioblastoma	179:190	arg1	infiltration					129:140	Diffuse infiltration	121:140	Diffuse infiltration across brain tissue	121:160	Diffuse infiltration across brain tissue is a hallmark of glioblastoma and the main cause of unsuccessful total resection that leads to tumor reappearance.
26161688	1	55	theme	glioblastoma	179:190	arg1	hallmark					167:174	a hallmark	165:174	a hallmark of glioblastoma	165:190	Diffuse infiltration across brain tissue is a hallmark of glioblastoma and the main cause of unsuccessful total resection that leads to tumor reappearance.
26161688	1	55	theme	glioblastoma	179:190	arg1	cause					205:209	the main cause	196:209	the main cause of unsuccessful total resection that leads to tumor reappearance	196:274	Diffuse infiltration across brain tissue is a hallmark of glioblastoma and the main cause of unsuccessful total resection that leads to tumor reappearance.
26161688	7	56	theme	coated	1418:1423	arg1	microfibers					1425:1435	Matrigel glass and coated microfibers	1399:1435	microfibers	1425:1435	The cells altered their migration mode depending on the matrix composition, showing migration by expansive growth in Matrigel matrices, multicellular extension along rigid interfaces (as Matrigel glass and coated microfibers), and mesenchymal single-cell migration in collagen matrices.
26161688	5	57	theme	used	884:887	arg1	formats					929:935	the most commonly used in vitro three-dimensional (3D) culture formats	866:935	the most commonly used in vitro three-dimensional (3D) culture formats	866:935	This model was compared with the most commonly used in vitro three-dimensional (3D) culture formats, Matrigel and collagen type-I monomer matrices, to study how the mechanical and compositional properties of the ECM alter the migration characteristics of GSC neurospheres.
26161688	9	58	theme	migratory	1684:1692	arg1	cells					1694:1698	migratory cells	1684:1698	migratory cells	1684:1698	In the dual niche system, the presence of HA reduced velocity and number of migratory cells; however, cells that came in contact with the pseudovessels exhibited collective migration by an extensive strand and reached higher velocities than cells migrating individually across the 3D matrix.
26161688	11	59	theme	single	2151:2156	arg1	migration					2173:2181	concomitant single and collective migration	2139:2181	concomitant single and collective migration as a function of the microenvironment topography to reach the most productive migration strategy	2139:2278	Moreover, GSC neurospheres exhibit concomitant single and collective migration as a function of the microenvironment topography to reach the most productive migration strategy.
26161688	1	60	theme	unsuccessful	214:225	arg1	resection					233:241	unsuccessful total resection	214:241	unsuccessful total resection that leads to tumor reappearance	214:274	Diffuse infiltration across brain tissue is a hallmark of glioblastoma and the main cause of unsuccessful total resection that leads to tumor reappearance.
26161688	5	61	theme	GSC	1092:1094	arg1	neurospheres					1096:1107	GSC neurospheres	1092:1107	GSC neurospheres	1092:1107	This model was compared with the most commonly used in vitro three-dimensional (3D) culture formats, Matrigel and collagen type-I monomer matrices, to study how the mechanical and compositional properties of the ECM alter the migration characteristics of GSC neurospheres.
26161688	3	62	theme	collagen-oligomer	578:594	arg1	network					603:609	a collagen-oligomer fibril network	576:609	a collagen-oligomer fibril network to simulate the brain tumor extracellular matrix (ECM) composition	576:676	In this study, we developed a composite matrix of hyaluronan (HA) structurally supported by a collagen-oligomer fibril network to simulate the brain tumor extracellular matrix (ECM) composition.
26161688	11	63	theme	GSC	2114:2116	arg1	neurospheres					2118:2129	GSC neurospheres	2114:2129	GSC neurospheres	2114:2129	Moreover, GSC neurospheres exhibit concomitant single and collective migration as a function of the microenvironment topography to reach the most productive migration strategy.
26161688	10	64	theme	ECM	1980:1982	arg1	microenvironments					1984:2000	multiple ECM microenvironments	1971:2000	multiple ECM microenvironments	1971:2000	Our results show that GSCs adopt varied migration mechanisms to invade multiple ECM microenvironments, and the migration characteristics exhibited are highly influenced by the matrix physical properties.
26161688	5	65	theme	ECM	1049:1051	arg1	properties					1031:1040	the mechanical and compositional properties	998:1040	the mechanical and compositional properties of the ECM	998:1051	This model was compared with the most commonly used in vitro three-dimensional (3D) culture formats, Matrigel and collagen type-I monomer matrices, to study how the mechanical and compositional properties of the ECM alter the migration characteristics of GSC neurospheres.
26161688	3	66	theme	brain	627:631	arg1	ECM					661:663	ECM	661:663	ECM	661:663	In this study, we developed a composite matrix of hyaluronan (HA) structurally supported by a collagen-oligomer fibril network to simulate the brain tumor extracellular matrix (ECM) composition.
26161688	3	66	theme	brain	627:631	arg1	matrix					653:658	the brain tumor extracellular matrix	623:658	the brain tumor extracellular matrix (ECM) composition	623:676	In this study, we developed a composite matrix of hyaluronan (HA) structurally supported by a collagen-oligomer fibril network to simulate the brain tumor extracellular matrix (ECM) composition.
26161688	1	67	theme	brain	149:153	arg1	tissue					155:160	brain tissue	149:160	brain tissue	149:160	Diffuse infiltration across brain tissue is a hallmark of glioblastoma and the main cause of unsuccessful total resection that leads to tumor reappearance.
26161688	7	68	theme	multicellular	1348:1360	arg1	extension					1362:1370	multicellular extension	1348:1370	multicellular extension along rigid interfaces (as Matrigel glass and coated microfibers)	1348:1436	The cells altered their migration mode depending on the matrix composition, showing migration by expansive growth in Matrigel matrices, multicellular extension along rigid interfaces (as Matrigel glass and coated microfibers), and mesenchymal single-cell migration in collagen matrices.
26161688	0	69	theme	Stem	80:83	arg1	Cells					85:89	Glioblastoma Stem Cells	67:89	Glioblastoma Stem Cells	67:89	Extracellular Matrix Properties Regulate the Migratory Response of Glioblastoma Stem Cells in Three-Dimensional Culture.
26161688	9	70	with	contact	1729:1735	arg1	pseudovessels					1746:1758	the pseudovessels	1742:1758	the pseudovessels	1742:1758	In the dual niche system, the presence of HA reduced velocity and number of migratory cells; however, cells that came in contact with the pseudovessels exhibited collective migration by an extensive strand and reached higher velocities than cells migrating individually across the 3D matrix.
26161688	5	71	theme	type-I	960:965	arg1	matrices					975:982	collagen type-I monomer matrices	951:982	collagen type-I monomer matrices	951:982	This model was compared with the most commonly used in vitro three-dimensional (3D) culture formats, Matrigel and collagen type-I monomer matrices, to study how the mechanical and compositional properties of the ECM alter the migration characteristics of GSC neurospheres.
26161688	11	72	theme	migration	2261:2269	arg1	strategy					2271:2278	the most productive migration strategy	2241:2278	the most productive migration strategy	2241:2278	Moreover, GSC neurospheres exhibit concomitant single and collective migration as a function of the microenvironment topography to reach the most productive migration strategy.
26161688	5	73	theme	monomer	967:973	arg1	matrices					975:982	collagen type-I monomer matrices	951:982	collagen type-I monomer matrices	951:982	This model was compared with the most commonly used in vitro three-dimensional (3D) culture formats, Matrigel and collagen type-I monomer matrices, to study how the mechanical and compositional properties of the ECM alter the migration characteristics of GSC neurospheres.
26161688	9	74	theme	collective	1770:1779	arg1	migration					1781:1789	collective migration	1770:1789	collective migration	1770:1789	In the dual niche system, the presence of HA reduced velocity and number of migratory cells; however, cells that came in contact with the pseudovessels exhibited collective migration by an extensive strand and reached higher velocities than cells migrating individually across the 3D matrix.
26161688	8	75	theme	migration	1524:1532	arg1	distance					1512:1519	distance	1512:1519	distance	1512:1519	Velocity and distance of migration within each composition varied according to matrix mechanical properties.
26161688	8	75	theme	migration	1524:1532	arg1	Velocity					1499:1506	Velocity	1499:1506	Velocity	1499:1506	Velocity and distance of migration within each composition varied according to matrix mechanical properties.
26161688	0	76	theme	Matrix	14:19	arg1	Properties					21:30	Extracellular Matrix Properties	0:30	Extracellular Matrix Properties	0:30	Extracellular Matrix Properties Regulate the Migratory Response of Glioblastoma Stem Cells in Three-Dimensional Culture.
26161688	9	77	theme	extensive	1797:1805	arg1	strand					1807:1812	an extensive strand	1794:1812	an extensive strand	1794:1812	In the dual niche system, the presence of HA reduced velocity and number of migratory cells; however, cells that came in contact with the pseudovessels exhibited collective migration by an extensive strand and reached higher velocities than cells migrating individually across the 3D matrix.
26161688	5	78	theme	collagen	951:958	arg1	matrices					975:982	collagen type-I monomer matrices	951:982	collagen type-I monomer matrices	951:982	This model was compared with the most commonly used in vitro three-dimensional (3D) culture formats, Matrigel and collagen type-I monomer matrices, to study how the mechanical and compositional properties of the ECM alter the migration characteristics of GSC neurospheres.
26161688	5	79	dep	three-dimensional	898:914	arg1	3D					917:918	3D	917:918	3D	917:918	This model was compared with the most commonly used in vitro three-dimensional (3D) culture formats, Matrigel and collagen type-I monomer matrices, to study how the mechanical and compositional properties of the ECM alter the migration characteristics of GSC neurospheres.
26161688	7	80	theme	migration	1236:1244	arg1	mode					1246:1249	their migration mode	1230:1249	their migration mode	1230:1249	The cells altered their migration mode depending on the matrix composition, showing migration by expansive growth in Matrigel matrices, multicellular extension along rigid interfaces (as Matrigel glass and coated microfibers), and mesenchymal single-cell migration in collagen matrices.
26161688	0	81	theme	Migratory	45:53	arg1	Response					55:62	the Migratory Response	41:62	the Migratory Response of Glioblastoma Stem Cells in Three-Dimensional Culture	41:118	Extracellular Matrix Properties Regulate the Migratory Response of Glioblastoma Stem Cells in Three-Dimensional Culture.
26161688	9	82	theme	higher	1826:1831	arg1	velocities					1833:1842	higher velocities	1826:1842	higher velocities	1826:1842	In the dual niche system, the presence of HA reduced velocity and number of migratory cells; however, cells that came in contact with the pseudovessels exhibited collective migration by an extensive strand and reached higher velocities than cells migrating individually across the 3D matrix.
26161688	8	83	theme	mechanical	1585:1594	arg1	properties					1596:1605	matrix mechanical properties	1578:1605	matrix mechanical properties	1578:1605	Velocity and distance of migration within each composition varied according to matrix mechanical properties.
26161688	3	84	theme	extracellular	639:651	arg1	ECM					661:663	ECM	661:663	ECM	661:663	In this study, we developed a composite matrix of hyaluronan (HA) structurally supported by a collagen-oligomer fibril network to simulate the brain tumor extracellular matrix (ECM) composition.
26161688	3	84	theme	extracellular	639:651	arg1	matrix					653:658	the brain tumor extracellular matrix	623:658	the brain tumor extracellular matrix (ECM) composition	623:676	In this study, we developed a composite matrix of hyaluronan (HA) structurally supported by a collagen-oligomer fibril network to simulate the brain tumor extracellular matrix (ECM) composition.
26161688	4	85	theme	brain	830:834	arg1	network					815:821	the vascular network	802:821	the vascular network of the brain	802:834	Matrigel-coated microfibers were embedded within the matrix to create a tunable dual niche microenvironment that resembles the vascular network of the brain.
26161688	9	86	theme	dual	1615:1618	arg1	system					1626:1631	the dual niche system	1611:1631	the dual niche system	1611:1631	In the dual niche system, the presence of HA reduced velocity and number of migratory cells; however, cells that came in contact with the pseudovessels exhibited collective migration by an extensive strand and reached higher velocities than cells migrating individually across the 3D matrix.
26161688	11	87	theme	topography	2221:2230	arg1	function					2188:2195	a function	2186:2195	a function of the microenvironment topography	2186:2230	Moreover, GSC neurospheres exhibit concomitant single and collective migration as a function of the microenvironment topography to reach the most productive migration strategy.
26161688	7	88	from	migration	1296:1304	arg1	matrices					1489:1496	collagen matrices	1480:1496	collagen matrices	1480:1496	The cells altered their migration mode depending on the matrix composition, showing migration by expansive growth in Matrigel matrices, multicellular extension along rigid interfaces (as Matrigel glass and coated microfibers), and mesenchymal single-cell migration in collagen matrices.
26161688	10	89	theme	physical	2083:2090	arg1	properties					2092:2101	the matrix physical properties	2072:2101	the matrix physical properties	2072:2101	Our results show that GSCs adopt varied migration mechanisms to invade multiple ECM microenvironments, and the migration characteristics exhibited are highly influenced by the matrix physical properties.
26161688	4	90	theme	vascular	806:813	arg1	network					815:821	the vascular network	802:821	the vascular network of the brain	802:834	Matrigel-coated microfibers were embedded within the matrix to create a tunable dual niche microenvironment that resembles the vascular network of the brain.
26161688	6	91	theme	migration	1114:1122	arg1	mode					1124:1127	The migration mode	1110:1127	The migration mode	1110:1127	The migration mode, distance, velocity, and morphology of the GSCs were monitored over a 72-h period.
25109455	5	0	theme	Culture	643:649	arg1	parameters					651:660	Culture parameters	643:660	Culture parameters	643:660	Culture parameters influenced both bacterial growth and EPS production, EPS yield always being directly related to the quantity of biomass.
25109455	8	1	theme	0.1	1032:1034	arg1	%					1035:1035	%	1035:1035	%	1035:1035	The crude EPS contained 44 ± 0.1% w/w carbohydrates and the following monosaccharide composition: glucose (24 ± 1.73), glucuronic acid (7.5 ± 0.37), mannose (5.5 ± 0.17), fucose (4.5 ± 0.36), galactose (1.2 ± 0.17) and rhamnose (1 ± 0.05) (%, w/w).
25109455	7	2	theme	×	994:994	arg1	masses					964:969	molecular masses	954:969	molecular masses of 8.2 × 10(4) and 1.4 × 10(6)	954:1000	The polymer was a sulphated heteropolysaccharide composed of two fractions, with molecular masses of 8.2 × 10(4) and 1.4 × 10(6).
25109455	2	3	theme	halophilic	350:359	arg1	bacteria					361:368	novel exopolysaccharide-producing halophilic bacteria	316:368	novel exopolysaccharide-producing halophilic bacteria	316:368	This strain was chosen during an ongoing research programme aimed at finding novel exopolysaccharide-producing halophilic bacteria in unexplored hypersaline habitats.
25109455	4	4	theme	growth	578:583	arg1	phase					585:589	the exponential growth phase	562:589	the exponential growth phase	562:589	It produced EPS mainly during the exponential growth phase and to a lesser extent during the stationary phase.
25109455	8	5	theme	monosaccharide	1073:1086	arg1	composition					1088:1098	the following monosaccharide composition	1059:1098	the following monosaccharide composition	1059:1098	The crude EPS contained 44 ± 0.1% w/w carbohydrates and the following monosaccharide composition: glucose (24 ± 1.73), glucuronic acid (7.5 ± 0.37), mannose (5.5 ± 0.17), fucose (4.5 ± 0.36), galactose (1.2 ± 0.17) and rhamnose (1 ± 0.05) (%, w/w).
25109455	7	6	theme	molecular	954:962	arg1	masses					964:969	molecular masses	954:969	molecular masses of 8.2 × 10(4) and 1.4 × 10(6)	954:1000	The polymer was a sulphated heteropolysaccharide composed of two fractions, with molecular masses of 8.2 × 10(4) and 1.4 × 10(6).
25109455	9	7	theme	behaviour	1310:1318	arg1	solutions					1264:1272	solutions	1264:1272	solutions of high viscosity and pseudoplastic behaviour that showed interesting flocculating and emulsifying activities and was also involved in forming biofilm	1264:1423	It produced solutions of high viscosity and pseudoplastic behaviour that showed interesting flocculating and emulsifying activities and was also involved in forming biofilm.
25109455	8	8	theme	following	1063:1071	arg1	composition					1088:1098	the following monosaccharide composition	1059:1098	the following monosaccharide composition	1059:1098	The crude EPS contained 44 ± 0.1% w/w carbohydrates and the following monosaccharide composition: glucose (24 ± 1.73), glucuronic acid (7.5 ± 0.37), mannose (5.5 ± 0.17), fucose (4.5 ± 0.36), galactose (1.2 ± 0.17) and rhamnose (1 ± 0.05) (%, w/w).
25109455	8	9	theme	±	1030:1030	arg1	carbohydrates					1041:1053	44 ± 0.1% w/w carbohydrates	1027:1053	44 ± 0.1% w/w carbohydrates	1027:1053	The crude EPS contained 44 ± 0.1% w/w carbohydrates and the following monosaccharide composition: glucose (24 ± 1.73), glucuronic acid (7.5 ± 0.37), mannose (5.5 ± 0.17), fucose (4.5 ± 0.36), galactose (1.2 ± 0.17) and rhamnose (1 ± 0.05) (%, w/w).
25109455	2	10	theme	exopolysaccharide-producing	322:348	arg1	bacteria					361:368	novel exopolysaccharide-producing halophilic bacteria	316:368	novel exopolysaccharide-producing halophilic bacteria	316:368	This strain was chosen during an ongoing research programme aimed at finding novel exopolysaccharide-producing halophilic bacteria in unexplored hypersaline habitats.
25109455	6	11	theme	culture	797:803	arg1	conditions					805:814	optimum culture conditions	789:814	optimum culture conditions	789:814	Under optimum culture conditions, strain HK30 produced 3.89 g of EPS per litre of medium.
25109455	6	12	theme	medium	865:870	arg1	litre					856:860	litre	856:860	litre of medium	856:870	Under optimum culture conditions, strain HK30 produced 3.89 g of EPS per litre of medium.
25109455	6	13	theme	optimum	789:795	arg1	conditions					805:814	optimum culture conditions	789:814	optimum culture conditions	789:814	Under optimum culture conditions, strain HK30 produced 3.89 g of EPS per litre of medium.
25109455	7	14	theme	×	978:978	arg1	masses					964:969	molecular masses	954:969	molecular masses of 8.2 × 10(4) and 1.4 × 10(6)	954:1000	The polymer was a sulphated heteropolysaccharide composed of two fractions, with molecular masses of 8.2 × 10(4) and 1.4 × 10(6).
25109455	9	15	theme	interesting	1332:1342	arg1	activities					1373:1382	interesting flocculating and emulsifying activities	1332:1382	interesting flocculating and emulsifying activities	1332:1382	It produced solutions of high viscosity and pseudoplastic behaviour that showed interesting flocculating and emulsifying activities and was also involved in forming biofilm.
25109455	9	16	theme	flocculating	1344:1355	arg1	activities					1373:1382	interesting flocculating and emulsifying activities	1332:1382	interesting flocculating and emulsifying activities	1332:1382	It produced solutions of high viscosity and pseudoplastic behaviour that showed interesting flocculating and emulsifying activities and was also involved in forming biofilm.
25109455	1	17	theme	strain	170:175	arg1	HK30					177:180	strain HK30	170:180	strain HK30 of Halomonas stenophila	170:204	We have conducted a thorough study of the exopolysaccharide (EPS) produced by strain HK30 of Halomonas stenophila, which we have named haloglycan.
25109455	7	18	with	heteropolysaccharide	901:920	arg1	masses					964:969	molecular masses	954:969	molecular masses of 8.2 × 10(4) and 1.4 × 10(6)	954:1000	The polymer was a sulphated heteropolysaccharide composed of two fractions, with molecular masses of 8.2 × 10(4) and 1.4 × 10(6).
25109455	3	19	dep	species	509:515	arg1	H.					517:518	H.	517:518	H.	517:518	Strain HK30 was isolated from a saline-wetland in Brikcha (Morocco) and identified as belonging to the species H. stenophila.
25109455	2	20	theme	hypersaline	384:394	arg1	habitats					396:403	unexplored hypersaline habitats	373:403	unexplored hypersaline habitats	373:403	This strain was chosen during an ongoing research programme aimed at finding novel exopolysaccharide-producing halophilic bacteria in unexplored hypersaline habitats.
25109455	8	21	theme	crude	1007:1011	arg1	EPS					1013:1015	The crude EPS	1003:1015	The crude EPS	1003:1015	The crude EPS contained 44 ± 0.1% w/w carbohydrates and the following monosaccharide composition: glucose (24 ± 1.73), glucuronic acid (7.5 ± 0.37), mannose (5.5 ± 0.17), fucose (4.5 ± 0.36), galactose (1.2 ± 0.17) and rhamnose (1 ± 0.05) (%, w/w).
25109455	3	22	dep	H.	517:518	arg1	stenophila					520:529	H. stenophila	517:529	the species H. stenophila	505:529	Strain HK30 was isolated from a saline-wetland in Brikcha (Morocco) and identified as belonging to the species H. stenophila.
25109455	4	23	theme	stationary	625:634	arg1	phase					636:640	the stationary phase	621:640	the stationary phase	621:640	It produced EPS mainly during the exponential growth phase and to a lesser extent during the stationary phase.
25109455	0	24	theme	haloglycan	20:29	arg1	exopolysaccharide					35:51	an exopolysaccharide	32:51	an exopolysaccharide produced by Halomonas stenophila HK30	32:89	Characterization of haloglycan, an exopolysaccharide produced by Halomonas stenophila HK30.
25109455	0	24	theme	haloglycan	20:29	arg1	Characterization					0:15	Characterization	0:15	Characterization of haloglycan	0:29	Characterization of haloglycan, an exopolysaccharide produced by Halomonas stenophila HK30.
25109455	4	25	theme	exponential	566:576	arg1	phase					585:589	the exponential growth phase	562:589	the exponential growth phase	562:589	It produced EPS mainly during the exponential growth phase and to a lesser extent during the stationary phase.
25109455	2	26	theme	ongoing	272:278	arg1	programme					289:297	an ongoing research programme	269:297	an ongoing research programme aimed at finding novel exopolysaccharide-producing halophilic bacteria in unexplored hypersaline habitats	269:403	This strain was chosen during an ongoing research programme aimed at finding novel exopolysaccharide-producing halophilic bacteria in unexplored hypersaline habitats.
25109455	9	27	theme	pseudoplastic	1296:1308	arg1	behaviour					1310:1318	pseudoplastic behaviour	1296:1318	pseudoplastic behaviour	1296:1318	It produced solutions of high viscosity and pseudoplastic behaviour that showed interesting flocculating and emulsifying activities and was also involved in forming biofilm.
25109455	1	28	theme	exopolysaccharide	134:150	arg1	study					121:125	a thorough study	110:125	a thorough study	110:125	We have conducted a thorough study of the exopolysaccharide (EPS) produced by strain HK30 of Halomonas stenophila, which we have named haloglycan.
25109455	5	29	theme	biomass	774:780	arg1	quantity					762:769	the quantity	758:769	the quantity of biomass	758:780	Culture parameters influenced both bacterial growth and EPS production, EPS yield always being directly related to the quantity of biomass.
25109455	5	30	theme	EPS	715:717	arg1	yield					719:723	EPS yield	715:723	EPS yield	715:723	Culture parameters influenced both bacterial growth and EPS production, EPS yield always being directly related to the quantity of biomass.
25109455	3	31	attach	isolated	422:429	arg2	Strain					406:411	Strain HK30	406:416	Strain HK30	406:416	Strain HK30 was isolated from a saline-wetland in Brikcha (Morocco) and identified as belonging to the species H. stenophila.
25109455	3	31	attach	isolated	422:429	arg1	saline-wetland					438:451	a saline-wetland	436:451	a saline-wetland in Brikcha (Morocco)	436:472	Strain HK30 was isolated from a saline-wetland in Brikcha (Morocco) and identified as belonging to the species H. stenophila.
25109455	1	32	theme	stenophila	195:204	arg1	HK30					177:180	strain HK30	170:180	strain HK30 of Halomonas stenophila	170:204	We have conducted a thorough study of the exopolysaccharide (EPS) produced by strain HK30 of Halomonas stenophila, which we have named haloglycan.
25109455	6	33	theme	EPS	848:850	arg1	g					843:843	3.89 g	838:843	3.89 g of EPS	838:850	Under optimum culture conditions, strain HK30 produced 3.89 g of EPS per litre of medium.
25109455	8	34	dep	fucose	1174:1179	arg1	w/w					1246:1248	w/w	1246:1248	w/w	1246:1248	The crude EPS contained 44 ± 0.1% w/w carbohydrates and the following monosaccharide composition: glucose (24 ± 1.73), glucuronic acid (7.5 ± 0.37), mannose (5.5 ± 0.17), fucose (4.5 ± 0.36), galactose (1.2 ± 0.17) and rhamnose (1 ± 0.05) (%, w/w).
25109455	8	34	dep	fucose	1174:1179	arg1	%					1243:1243	%	1243:1243	%	1243:1243	The crude EPS contained 44 ± 0.1% w/w carbohydrates and the following monosaccharide composition: glucose (24 ± 1.73), glucuronic acid (7.5 ± 0.37), mannose (5.5 ± 0.17), fucose (4.5 ± 0.36), galactose (1.2 ± 0.17) and rhamnose (1 ± 0.05) (%, w/w).
25109455	8	35	theme	glucuronic	1122:1131	arg1	acid					1133:1136	glucuronic acid	1122:1136	glucuronic acid (7.5 ± 0.37)	1122:1149	The crude EPS contained 44 ± 0.1% w/w carbohydrates and the following monosaccharide composition: glucose (24 ± 1.73), glucuronic acid (7.5 ± 0.37), mannose (5.5 ± 0.17), fucose (4.5 ± 0.36), galactose (1.2 ± 0.17) and rhamnose (1 ± 0.05) (%, w/w).
25109455	8	35	theme	glucuronic	1122:1131	arg1	±					1143:1143	7.5 ± 0.37	1139:1148	7.5 ± 0.37	1139:1148	The crude EPS contained 44 ± 0.1% w/w carbohydrates and the following monosaccharide composition: glucose (24 ± 1.73), glucuronic acid (7.5 ± 0.37), mannose (5.5 ± 0.17), fucose (4.5 ± 0.36), galactose (1.2 ± 0.17) and rhamnose (1 ± 0.05) (%, w/w).
25109455	8	35	theme	glucuronic	1122:1131	arg1	glucose					1101:1107	glucose	1101:1107	glucose (24 ± 1.73)	1101:1119	The crude EPS contained 44 ± 0.1% w/w carbohydrates and the following monosaccharide composition: glucose (24 ± 1.73), glucuronic acid (7.5 ± 0.37), mannose (5.5 ± 0.17), fucose (4.5 ± 0.36), galactose (1.2 ± 0.17) and rhamnose (1 ± 0.05) (%, w/w).
25109455	5	36	theme	bacterial	678:686	arg1	growth					688:693	bacterial growth	678:693	bacterial growth	678:693	Culture parameters influenced both bacterial growth and EPS production, EPS yield always being directly related to the quantity of biomass.
25109455	8	37	theme	w/w	1037:1039	arg1	carbohydrates					1041:1053	44 ± 0.1% w/w carbohydrates	1027:1053	44 ± 0.1% w/w carbohydrates	1027:1053	The crude EPS contained 44 ± 0.1% w/w carbohydrates and the following monosaccharide composition: glucose (24 ± 1.73), glucuronic acid (7.5 ± 0.37), mannose (5.5 ± 0.17), fucose (4.5 ± 0.36), galactose (1.2 ± 0.17) and rhamnose (1 ± 0.05) (%, w/w).
25109455	1	38	theme	thorough	112:119	arg1	study					121:125	a thorough study	110:125	a thorough study	110:125	We have conducted a thorough study of the exopolysaccharide (EPS) produced by strain HK30 of Halomonas stenophila, which we have named haloglycan.
25109455	9	39	theme	high	1277:1280	arg1	viscosity					1282:1290	high viscosity	1277:1290	high viscosity	1277:1290	It produced solutions of high viscosity and pseudoplastic behaviour that showed interesting flocculating and emulsifying activities and was also involved in forming biofilm.
25109455	2	40	theme	novel	316:320	arg1	bacteria					361:368	novel exopolysaccharide-producing halophilic bacteria	316:368	novel exopolysaccharide-producing halophilic bacteria	316:368	This strain was chosen during an ongoing research programme aimed at finding novel exopolysaccharide-producing halophilic bacteria in unexplored hypersaline habitats.
25109455	8	41	theme	%	1035:1035	arg1	carbohydrates					1041:1053	44 ± 0.1% w/w carbohydrates	1027:1053	44 ± 0.1% w/w carbohydrates	1027:1053	The crude EPS contained 44 ± 0.1% w/w carbohydrates and the following monosaccharide composition: glucose (24 ± 1.73), glucuronic acid (7.5 ± 0.37), mannose (5.5 ± 0.17), fucose (4.5 ± 0.36), galactose (1.2 ± 0.17) and rhamnose (1 ± 0.05) (%, w/w).
25109455	2	42	theme	research	280:287	arg1	programme					289:297	an ongoing research programme	269:297	an ongoing research programme aimed at finding novel exopolysaccharide-producing halophilic bacteria in unexplored hypersaline habitats	269:403	This strain was chosen during an ongoing research programme aimed at finding novel exopolysaccharide-producing halophilic bacteria in unexplored hypersaline habitats.
25109455	4	43	theme	lesser	600:605	arg1	extent					607:612	a lesser extent	598:612	a lesser extent	598:612	It produced EPS mainly during the exponential growth phase and to a lesser extent during the stationary phase.
25109455	5	44	theme	EPS	699:701	arg1	production					703:712	EPS production	699:712	EPS production	699:712	Culture parameters influenced both bacterial growth and EPS production, EPS yield always being directly related to the quantity of biomass.
25109455	9	45	theme	viscosity	1282:1290	arg1	solutions					1264:1272	solutions	1264:1272	solutions of high viscosity and pseudoplastic behaviour that showed interesting flocculating and emulsifying activities and was also involved in forming biofilm	1264:1423	It produced solutions of high viscosity and pseudoplastic behaviour that showed interesting flocculating and emulsifying activities and was also involved in forming biofilm.
25109455	0	46	theme	stenophila	75:84	arg1	HK30					86:89	Halomonas stenophila HK30	65:89	Halomonas stenophila HK30	65:89	Characterization of haloglycan, an exopolysaccharide produced by Halomonas stenophila HK30.
25109455	3	47	from	saline-wetland	438:451	arg1	Morocco					465:471	Morocco	465:471	Morocco	465:471	Strain HK30 was isolated from a saline-wetland in Brikcha (Morocco) and identified as belonging to the species H. stenophila.
25109455	2	48	theme	unexplored	373:382	arg1	habitats					396:403	unexplored hypersaline habitats	373:403	unexplored hypersaline habitats	373:403	This strain was chosen during an ongoing research programme aimed at finding novel exopolysaccharide-producing halophilic bacteria in unexplored hypersaline habitats.
25109455	7	49	theme	sulphated	891:899	arg1	heteropolysaccharide					901:920	a sulphated heteropolysaccharide	889:920	a sulphated heteropolysaccharide composed of two fractions	889:946	The polymer was a sulphated heteropolysaccharide composed of two fractions, with molecular masses of 8.2 × 10(4) and 1.4 × 10(6).
25109455	7	49	theme	sulphated	891:899	arg1	polymer					877:883	The polymer	873:883	The polymer	873:883	The polymer was a sulphated heteropolysaccharide composed of two fractions, with molecular masses of 8.2 × 10(4) and 1.4 × 10(6).
25109455	0	50	theme	Halomonas	65:73	arg1	HK30					86:89	Halomonas stenophila HK30	65:89	Halomonas stenophila HK30	65:89	Characterization of haloglycan, an exopolysaccharide produced by Halomonas stenophila HK30.
25109455	9	51	theme	emulsifying	1361:1371	arg1	activities					1373:1382	interesting flocculating and emulsifying activities	1332:1382	interesting flocculating and emulsifying activities	1332:1382	It produced solutions of high viscosity and pseudoplastic behaviour that showed interesting flocculating and emulsifying activities and was also involved in forming biofilm.
25109455	8	52	contain	contained	1017:1025	arg1	EPS					1013:1015	The crude EPS	1003:1015	The crude EPS	1003:1015	The crude EPS contained 44 ± 0.1% w/w carbohydrates and the following monosaccharide composition: glucose (24 ± 1.73), glucuronic acid (7.5 ± 0.37), mannose (5.5 ± 0.17), fucose (4.5 ± 0.36), galactose (1.2 ± 0.17) and rhamnose (1 ± 0.05) (%, w/w).
25109455	8	52	contain	contained	1017:1025	arg2	composition					1088:1098	the following monosaccharide composition	1059:1098	the following monosaccharide composition	1059:1098	The crude EPS contained 44 ± 0.1% w/w carbohydrates and the following monosaccharide composition: glucose (24 ± 1.73), glucuronic acid (7.5 ± 0.37), mannose (5.5 ± 0.17), fucose (4.5 ± 0.36), galactose (1.2 ± 0.17) and rhamnose (1 ± 0.05) (%, w/w).
25109455	8	52	contain	contained	1017:1025	arg2	carbohydrates					1041:1053	44 ± 0.1% w/w carbohydrates	1027:1053	44 ± 0.1% w/w carbohydrates	1027:1053	The crude EPS contained 44 ± 0.1% w/w carbohydrates and the following monosaccharide composition: glucose (24 ± 1.73), glucuronic acid (7.5 ± 0.37), mannose (5.5 ± 0.17), fucose (4.5 ± 0.36), galactose (1.2 ± 0.17) and rhamnose (1 ± 0.05) (%, w/w).
27770231	8	0	theme	cell	1128:1131	arg1	components					1138:1147	various cell wall components	1120:1147	various cell wall components	1120:1147	This modification is achieved via several strategies, including, among others, altering biosynthetic pathways and modifying the associations and structures of various cell wall components.
27770231	5	1	theme	bioethanol	628:637	arg1	production					614:623	the production	610:623	the production of bioethanol in terms of saccharification of essential substrates	610:690	Our results suggest that the transgenic plants have an advantage for the production of bioethanol in terms of saccharification of essential substrates.
27770231	10	2	theme	increased	1354:1362	arg1	biomass					1364:1370	an increased biomass	1351:1370	an increased biomass	1351:1370	The resulting transgenic plants (E8-SDB123) showed an increased biomass, higher levels of both fermentable sugars and hydrolyzed cellulose and altered cell wall properties such as higher laxity and degradability, which are valuable characteristics for the second-generation biofuels industry.
27770231	0	3	from	synthase	52:59	arg1	domains					32:38	starch binding domains	17:38	starch binding domains from starch synthase III	17:63	The targeting of starch binding domains from starch synthase III to the cell wall alters cell wall composition and properties.
27770231	0	3	from	synthase	52:59	arg1	targeting					4:12	The targeting	0:12	The targeting of starch binding domains from starch synthase III to the cell wall	0:80	The targeting of starch binding domains from starch synthase III to the cell wall alters cell wall composition and properties.
27770231	1	4	theme	cell	232:235	arg1	polysaccharides					242:256	cell wall polysaccharides	232:256	cell wall polysaccharides	232:256	Starch binding domains of starch synthase III from Arabidopsis thaliana (SBD123) binds preferentially to cell wall polysaccharides rather than to starch in vitro.
27770231	8	5	theme	components	1138:1147	arg1	structures					1106:1115	structures	1106:1115	structures	1106:1115	This modification is achieved via several strategies, including, among others, altering biosynthetic pathways and modifying the associations and structures of various cell wall components.
27770231	8	5	theme	components	1138:1147	arg1	associations					1089:1100	associations	1089:1100	associations	1089:1100	This modification is achieved via several strategies, including, among others, altering biosynthetic pathways and modifying the associations and structures of various cell wall components.
27770231	10	6	theme	transgenic	1314:1323	arg1	plants					1325:1330	transgenic plants	1314:1330	The resulting transgenic plants (E8-SDB123)	1300:1342	The resulting transgenic plants (E8-SDB123) showed an increased biomass, higher levels of both fermentable sugars and hydrolyzed cellulose and altered cell wall properties such as higher laxity and degradability, which are valuable characteristics for the second-generation biofuels industry.
27770231	10	6	theme	transgenic	1314:1323	arg1	E8-SDB123					1333:1341	E8-SDB123	1333:1341	E8-SDB123	1333:1341	The resulting transgenic plants (E8-SDB123) showed an increased biomass, higher levels of both fermentable sugars and hydrolyzed cellulose and altered cell wall properties such as higher laxity and degradability, which are valuable characteristics for the second-generation biofuels industry.
27770231	12	7	dep	reducing	1852:1859	arg1	therefore					1890:1898	therefore	1890:1898	therefore	1890:1898	Based on these results, our approach represents a promising biotechnological tool for reducing of biomass recalcitrance and therefore, the need for pretreatments.
27770231	12	7	dep	reducing	1852:1859	arg1	recalcitrance					1872:1884	biomass recalcitrance	1864:1884	biomass recalcitrance	1864:1884	Based on these results, our approach represents a promising biotechnological tool for reducing of biomass recalcitrance and therefore, the need for pretreatments.
27770231	11	8	theme	E8-SBD123	1646:1654	arg1	plants					1656:1661	E8-SBD123 plants	1646:1661	E8-SBD123 plants	1646:1661	The increased biomass and degradability phenotype of E8-SBD123 plants could be explained by the putative cell-wall loosening effect of the in tandem starch binding domains.
27770231	6	9	theme	biofuel	761:767	arg1	production					769:778	biofuel production	761:778	biofuel production	761:778	The plant cell wall, which represents a major source of biomass for biofuel production, is composed of cellulose, hemicelluloses, pectins and lignin.
27770231	1	10	theme	starch	153:158	arg1	synthase					160:167	starch synthase III	153:171	starch synthase III from Arabidopsis thaliana (SBD123)	153:206	Starch binding domains of starch synthase III from Arabidopsis thaliana (SBD123) binds preferentially to cell wall polysaccharides rather than to starch in vitro.
27770231	8	11	dep	associations	1089:1100	arg1	the					1085:1087	the	1085:1087	the	1085:1087	This modification is achieved via several strategies, including, among others, altering biosynthetic pathways and modifying the associations and structures of various cell wall components.
27770231	6	12	theme	biomass	749:755	arg1	source					739:744	a major source	731:744	a major source of biomass for biofuel production	731:778	The plant cell wall, which represents a major source of biomass for biofuel production, is composed of cellulose, hemicelluloses, pectins and lignin.
27770231	0	13	theme	wall	94:97	arg1	composition					99:109	cell wall composition	89:109	cell wall composition	89:109	The targeting of starch binding domains from starch synthase III to the cell wall alters cell wall composition and properties.
27770231	1	14	theme	Arabidopsis	178:188	arg1	SBD123					200:205	SBD123	200:205	SBD123	200:205	Starch binding domains of starch synthase III from Arabidopsis thaliana (SBD123) binds preferentially to cell wall polysaccharides rather than to starch in vitro.
27770231	1	14	theme	Arabidopsis	178:188	arg1	thaliana					190:197	Arabidopsis thaliana	178:197	Arabidopsis thaliana (SBD123)	178:206	Starch binding domains of starch synthase III from Arabidopsis thaliana (SBD123) binds preferentially to cell wall polysaccharides rather than to starch in vitro.
27770231	9	15	theme	thaliana	1197:1204	arg1	wall					1186:1189	the cell wall	1177:1189	the cell wall of A. thaliana	1177:1204	In this study, we modified the cell wall of A. thaliana by targeting the starch-binding domains of A. thaliana starch synthase III to this structure.
27770231	1	16	from	thaliana	190:197	arg1	synthase					160:167	starch synthase III	153:171	starch synthase III from Arabidopsis thaliana (SBD123)	153:206	Starch binding domains of starch synthase III from Arabidopsis thaliana (SBD123) binds preferentially to cell wall polysaccharides rather than to starch in vitro.
27770231	1	16	from	thaliana	190:197	arg1	domains					142:148	Starch binding domains	127:148	Starch binding domains of starch synthase III from Arabidopsis thaliana (SBD123)	127:206	Starch binding domains of starch synthase III from Arabidopsis thaliana (SBD123) binds preferentially to cell wall polysaccharides rather than to starch in vitro.
27770231	4	17	theme	Transgenic	429:438	arg1	plants					440:445	Transgenic plants	429:445	Transgenic plants	429:445	Transgenic plants are more susceptible to digestion than wild type and present higher released glucose content.
27770231	5	18	theme	saccharification	651:666	arg1	terms					642:646	terms	642:646	terms of saccharification of essential substrates	642:690	Our results suggest that the transgenic plants have an advantage for the production of bioethanol in terms of saccharification of essential substrates.
27770231	11	19	theme	tandem	1735:1740	arg1	domains					1757:1763	the in tandem starch binding domains	1728:1763	the in tandem starch binding domains	1728:1763	The increased biomass and degradability phenotype of E8-SBD123 plants could be explained by the putative cell-wall loosening effect of the in tandem starch binding domains.
27770231	5	20	theme	essential	671:679	arg1	substrates					681:690	essential substrates	671:690	essential substrates	671:690	Our results suggest that the transgenic plants have an advantage for the production of bioethanol in terms of saccharification of essential substrates.
27770231	9	21	theme	starch-binding	1223:1236	arg1	synthase					1268:1275	A. thaliana starch synthase III	1249:1279	A. thaliana starch synthase III	1249:1279	In this study, we modified the cell wall of A. thaliana by targeting the starch-binding domains of A. thaliana starch synthase III to this structure.
27770231	9	21	theme	starch-binding	1223:1236	arg1	domains					1238:1244	the starch-binding domains	1219:1244	the starch-binding domains of A. thaliana starch synthase III	1219:1279	In this study, we modified the cell wall of A. thaliana by targeting the starch-binding domains of A. thaliana starch synthase III to this structure.
27770231	7	22	theme	cell	949:952	arg1	walls					954:958	plant cell walls	943:958	plant cell walls	943:958	A potential biotechnological target for improving the production of biofuels is the modification of plant cell walls.
27770231	1	23	theme	Starch	127:132	arg1	synthase					160:167	starch synthase III	153:171	starch synthase III from Arabidopsis thaliana (SBD123)	153:206	Starch binding domains of starch synthase III from Arabidopsis thaliana (SBD123) binds preferentially to cell wall polysaccharides rather than to starch in vitro.
27770231	1	23	theme	Starch	127:132	arg1	domains					142:148	Starch binding domains	127:148	Starch binding domains of starch synthase III from Arabidopsis thaliana (SBD123)	127:206	Starch binding domains of starch synthase III from Arabidopsis thaliana (SBD123) binds preferentially to cell wall polysaccharides rather than to starch in vitro.
27770231	10	24	theme	sugars	1407:1412	arg1	levels					1380:1385	higher levels	1373:1385	higher levels of both fermentable sugars	1373:1412	The resulting transgenic plants (E8-SDB123) showed an increased biomass, higher levels of both fermentable sugars and hydrolyzed cellulose and altered cell wall properties such as higher laxity and degradability, which are valuable characteristics for the second-generation biofuels industry.
27770231	10	25	dep	higher	1480:1485	arg1	degradability					1498:1510	degradability	1498:1510	degradability	1498:1510	The resulting transgenic plants (E8-SDB123) showed an increased biomass, higher levels of both fermentable sugars and hydrolyzed cellulose and altered cell wall properties such as higher laxity and degradability, which are valuable characteristics for the second-generation biofuels industry.
27770231	10	25	dep	higher	1480:1485	arg1	laxity					1487:1492	laxity	1487:1492	laxity	1487:1492	The resulting transgenic plants (E8-SDB123) showed an increased biomass, higher levels of both fermentable sugars and hydrolyzed cellulose and altered cell wall properties such as higher laxity and degradability, which are valuable characteristics for the second-generation biofuels industry.
27770231	4	26	dep	present	500:506	arg1	higher					508:513	higher	508:513	higher	508:513	Transgenic plants are more susceptible to digestion than wild type and present higher released glucose content.
27770231	8	27	theme	several	995:1001	arg1	strategies					1003:1012	several strategies	995:1012	several strategies	995:1012	This modification is achieved via several strategies, including, among others, altering biosynthetic pathways and modifying the associations and structures of various cell wall components.
27770231	5	28	theme	transgenic	570:579	arg1	plants					581:586	the transgenic plants	566:586	the transgenic plants	566:586	Our results suggest that the transgenic plants have an advantage for the production of bioethanol in terms of saccharification of essential substrates.
27770231	4	29	theme	wild	486:489	arg1	type					491:494	wild type	486:494	wild type	486:494	Transgenic plants are more susceptible to digestion than wild type and present higher released glucose content.
27770231	11	30	theme	binding	1749:1755	arg1	domains					1757:1763	the in tandem starch binding domains	1728:1763	the in tandem starch binding domains	1728:1763	The increased biomass and degradability phenotype of E8-SBD123 plants could be explained by the putative cell-wall loosening effect of the in tandem starch binding domains.
27770231	0	31	theme	cell	72:75	arg1	wall					77:80	the cell wall	68:80	the cell wall	68:80	The targeting of starch binding domains from starch synthase III to the cell wall alters cell wall composition and properties.
27770231	9	32	theme	synthase	1268:1275	arg1	synthase					1268:1275	A. thaliana starch synthase III	1249:1279	A. thaliana starch synthase III	1249:1279	In this study, we modified the cell wall of A. thaliana by targeting the starch-binding domains of A. thaliana starch synthase III to this structure.
27770231	9	32	theme	synthase	1268:1275	arg1	domains					1238:1244	the starch-binding domains	1219:1244	the starch-binding domains of A. thaliana starch synthase III	1219:1279	In this study, we modified the cell wall of A. thaliana by targeting the starch-binding domains of A. thaliana starch synthase III to this structure.
27770231	7	33	theme	biofuels	911:918	arg1	production					897:906	the production	893:906	the production of biofuels	893:918	A potential biotechnological target for improving the production of biofuels is the modification of plant cell walls.
27770231	10	34	theme	valuable	1523:1530	arg1	higher					1480:1485	higher	1480:1485	higher	1480:1485	The resulting transgenic plants (E8-SDB123) showed an increased biomass, higher levels of both fermentable sugars and hydrolyzed cellulose and altered cell wall properties such as higher laxity and degradability, which are valuable characteristics for the second-generation biofuels industry.
27770231	10	34	theme	valuable	1523:1530	arg1	characteristics					1532:1546	valuable characteristics	1523:1546	valuable characteristics for the second-generation biofuels industry	1523:1590	The resulting transgenic plants (E8-SDB123) showed an increased biomass, higher levels of both fermentable sugars and hydrolyzed cellulose and altered cell wall properties such as higher laxity and degradability, which are valuable characteristics for the second-generation biofuels industry.
27770231	4	35	theme	glucose	524:530	arg1	content					532:538	present higher released glucose content	500:538	present higher released glucose content	500:538	Transgenic plants are more susceptible to digestion than wild type and present higher released glucose content.
27770231	3	36	theme	wall	379:382	arg1	components					384:393	Cell wall components	374:393	Cell wall components	374:393	Cell wall components are altered in transgenic plants.
27770231	9	37	dep	A.	1249:1250	arg1	thaliana					1252:1259	thaliana	1252:1259	thaliana	1252:1259	In this study, we modified the cell wall of A. thaliana by targeting the starch-binding domains of A. thaliana starch synthase III to this structure.
27770231	10	38	theme	cell	1451:1454	arg1	properties					1461:1470	cell wall properties	1451:1470	cell wall properties such as higher laxity and degradability, which are valuable characteristics for the second-generation biofuels industry	1451:1590	The resulting transgenic plants (E8-SDB123) showed an increased biomass, higher levels of both fermentable sugars and hydrolyzed cellulose and altered cell wall properties such as higher laxity and degradability, which are valuable characteristics for the second-generation biofuels industry.
27770231	10	38	theme	cell	1451:1454	arg1	higher					1480:1485	higher	1480:1485	higher	1480:1485	The resulting transgenic plants (E8-SDB123) showed an increased biomass, higher levels of both fermentable sugars and hydrolyzed cellulose and altered cell wall properties such as higher laxity and degradability, which are valuable characteristics for the second-generation biofuels industry.
27770231	11	39	theme	putative	1689:1696	arg1	effect					1718:1723	the putative cell-wall loosening effect	1685:1723	the putative cell-wall loosening effect of the in tandem starch binding domains	1685:1763	The increased biomass and degradability phenotype of E8-SBD123 plants could be explained by the putative cell-wall loosening effect of the in tandem starch binding domains.
27770231	0	40	theme	binding	24:30	arg1	domains					32:38	starch binding domains	17:38	starch binding domains from starch synthase III	17:63	The targeting of starch binding domains from starch synthase III to the cell wall alters cell wall composition and properties.
27770231	12	41	theme	biomass	1864:1870	arg1	recalcitrance					1872:1884	biomass recalcitrance	1864:1884	biomass recalcitrance	1864:1884	Based on these results, our approach represents a promising biotechnological tool for reducing of biomass recalcitrance and therefore, the need for pretreatments.
27770231	6	42	theme	major	733:737	arg1	source					739:744	a major source	731:744	a major source of biomass for biofuel production	731:778	The plant cell wall, which represents a major source of biomass for biofuel production, is composed of cellulose, hemicelluloses, pectins and lignin.
27770231	12	43	theme	promising	1816:1824	arg1	tool					1843:1846	a promising biotechnological tool	1814:1846	a promising biotechnological tool for reducing of biomass recalcitrance and therefore, the need for pretreatments	1814:1926	Based on these results, our approach represents a promising biotechnological tool for reducing of biomass recalcitrance and therefore, the need for pretreatments.
27770231	11	44	theme	loosening	1708:1716	arg1	effect					1718:1723	the putative cell-wall loosening effect	1685:1723	the putative cell-wall loosening effect of the in tandem starch binding domains	1685:1763	The increased biomass and degradability phenotype of E8-SBD123 plants could be explained by the putative cell-wall loosening effect of the in tandem starch binding domains.
27770231	11	45	theme	biomass	1607:1613	arg1	phenotype					1633:1641	The increased biomass and degradability phenotype	1593:1641	The increased biomass and degradability phenotype of E8-SBD123 plants	1593:1661	The increased biomass and degradability phenotype of E8-SBD123 plants could be explained by the putative cell-wall loosening effect of the in tandem starch binding domains.
27770231	6	46	theme	cell	703:706	arg1	wall					708:711	The plant cell wall	693:711	The plant cell wall	693:711	The plant cell wall, which represents a major source of biomass for biofuel production, is composed of cellulose, hemicelluloses, pectins and lignin.
27770231	2	47	theme	cell	337:340	arg1	wall					342:345	the cell wall	333:345	the cell wall	333:345	Transgenic plants overexpressing SBD123 in the cell wall are larger than wild type.
27770231	7	48	theme	biotechnological	855:870	arg1	modification					927:938	the modification	923:938	the modification of plant cell walls	923:958	A potential biotechnological target for improving the production of biofuels is the modification of plant cell walls.
27770231	7	48	theme	biotechnological	855:870	arg1	target					872:877	A potential biotechnological target	843:877	A potential biotechnological target for improving the production of biofuels	843:918	A potential biotechnological target for improving the production of biofuels is the modification of plant cell walls.
27770231	8	49	theme	various	1120:1126	arg1	components					1138:1147	various cell wall components	1120:1147	various cell wall components	1120:1147	This modification is achieved via several strategies, including, among others, altering biosynthetic pathways and modifying the associations and structures of various cell wall components.
27770231	11	50	theme	degradability	1619:1631	arg1	phenotype					1633:1641	The increased biomass and degradability phenotype	1593:1641	The increased biomass and degradability phenotype of E8-SBD123 plants	1593:1661	The increased biomass and degradability phenotype of E8-SBD123 plants could be explained by the putative cell-wall loosening effect of the in tandem starch binding domains.
27770231	1	51	theme	wall	237:240	arg1	polysaccharides					242:256	cell wall polysaccharides	232:256	cell wall polysaccharides	232:256	Starch binding domains of starch synthase III from Arabidopsis thaliana (SBD123) binds preferentially to cell wall polysaccharides rather than to starch in vitro.
27770231	8	52	theme	wall	1133:1136	arg1	components					1138:1147	various cell wall components	1120:1147	various cell wall components	1120:1147	This modification is achieved via several strategies, including, among others, altering biosynthetic pathways and modifying the associations and structures of various cell wall components.
27770231	8	53	dep	strategies	1003:1012	arg1	altering					1040:1047	altering	1040:1047	altering biosynthetic pathways	1040:1069	This modification is achieved via several strategies, including, among others, altering biosynthetic pathways and modifying the associations and structures of various cell wall components.
27770231	8	53	dep	strategies	1003:1012	arg1	including					1015:1023	including	1015:1023	including	1015:1023	This modification is achieved via several strategies, including, among others, altering biosynthetic pathways and modifying the associations and structures of various cell wall components.
27770231	8	53	dep	strategies	1003:1012	arg1	others					1032:1037	others	1032:1037	others	1032:1037	This modification is achieved via several strategies, including, among others, altering biosynthetic pathways and modifying the associations and structures of various cell wall components.
27770231	8	53	dep	strategies	1003:1012	arg1	modifying					1075:1083	modifying	1075:1083	modifying the associations and structures of various cell wall components	1075:1147	This modification is achieved via several strategies, including, among others, altering biosynthetic pathways and modifying the associations and structures of various cell wall components.
27770231	10	54	theme	biofuels	1574:1581	arg1	industry					1583:1590	the second-generation biofuels industry	1552:1590	the second-generation biofuels industry	1552:1590	The resulting transgenic plants (E8-SDB123) showed an increased biomass, higher levels of both fermentable sugars and hydrolyzed cellulose and altered cell wall properties such as higher laxity and degradability, which are valuable characteristics for the second-generation biofuels industry.
27770231	5	55	contain	have	588:591	arg1	plants					581:586	the transgenic plants	566:586	the transgenic plants	566:586	Our results suggest that the transgenic plants have an advantage for the production of bioethanol in terms of saccharification of essential substrates.
27770231	5	55	contain	have	588:591	arg2	advantage					596:604	an advantage	593:604	an advantage for the production of bioethanol in terms of saccharification of essential substrates	593:690	Our results suggest that the transgenic plants have an advantage for the production of bioethanol in terms of saccharification of essential substrates.
27770231	11	56	theme	plants	1656:1661	arg1	phenotype					1633:1641	The increased biomass and degradability phenotype	1593:1641	The increased biomass and degradability phenotype of E8-SBD123 plants	1593:1661	The increased biomass and degradability phenotype of E8-SBD123 plants could be explained by the putative cell-wall loosening effect of the in tandem starch binding domains.
27770231	10	57	theme	higher	1373:1378	arg1	levels					1380:1385	higher levels	1373:1385	higher levels of both fermentable sugars	1373:1412	The resulting transgenic plants (E8-SDB123) showed an increased biomass, higher levels of both fermentable sugars and hydrolyzed cellulose and altered cell wall properties such as higher laxity and degradability, which are valuable characteristics for the second-generation biofuels industry.
27770231	2	58	theme	Transgenic	290:299	arg1	plants					301:306	Transgenic plants	290:306	Transgenic plants overexpressing SBD123 in the cell wall	290:345	Transgenic plants overexpressing SBD123 in the cell wall are larger than wild type.
27770231	1	59	theme	synthase	160:167	arg1	synthase					160:167	starch synthase III	153:171	starch synthase III from Arabidopsis thaliana (SBD123)	153:206	Starch binding domains of starch synthase III from Arabidopsis thaliana (SBD123) binds preferentially to cell wall polysaccharides rather than to starch in vitro.
27770231	1	59	theme	synthase	160:167	arg1	domains					142:148	Starch binding domains	127:148	Starch binding domains of starch synthase III from Arabidopsis thaliana (SBD123)	127:206	Starch binding domains of starch synthase III from Arabidopsis thaliana (SBD123) binds preferentially to cell wall polysaccharides rather than to starch in vitro.
27770231	10	60	theme	second-generation	1556:1572	arg1	industry					1583:1590	the second-generation biofuels industry	1552:1590	the second-generation biofuels industry	1552:1590	The resulting transgenic plants (E8-SDB123) showed an increased biomass, higher levels of both fermentable sugars and hydrolyzed cellulose and altered cell wall properties such as higher laxity and degradability, which are valuable characteristics for the second-generation biofuels industry.
27770231	9	61	theme	cell	1181:1184	arg1	wall					1186:1189	the cell wall	1177:1189	the cell wall of A. thaliana	1177:1204	In this study, we modified the cell wall of A. thaliana by targeting the starch-binding domains of A. thaliana starch synthase III to this structure.
27770231	5	62	theme	substrates	681:690	arg1	saccharification					651:666	saccharification	651:666	saccharification of essential substrates	651:690	Our results suggest that the transgenic plants have an advantage for the production of bioethanol in terms of saccharification of essential substrates.
27770231	7	63	theme	plant	943:947	arg1	walls					954:958	plant cell walls	943:958	plant cell walls	943:958	A potential biotechnological target for improving the production of biofuels is the modification of plant cell walls.
27770231	11	64	theme	starch	1742:1747	arg1	domains					1757:1763	the in tandem starch binding domains	1728:1763	the in tandem starch binding domains	1728:1763	The increased biomass and degradability phenotype of E8-SBD123 plants could be explained by the putative cell-wall loosening effect of the in tandem starch binding domains.
27770231	11	65	theme	in	1732:1733	arg1	domains					1757:1763	the in tandem starch binding domains	1728:1763	the in tandem starch binding domains	1728:1763	The increased biomass and degradability phenotype of E8-SBD123 plants could be explained by the putative cell-wall loosening effect of the in tandem starch binding domains.
27770231	9	66	theme	A.	1249:1250	arg1	synthase					1268:1275	A. thaliana starch synthase III	1249:1279	A. thaliana starch synthase III	1249:1279	In this study, we modified the cell wall of A. thaliana by targeting the starch-binding domains of A. thaliana starch synthase III to this structure.
27770231	7	67	theme	walls	954:958	arg1	modification					927:938	the modification	923:938	the modification of plant cell walls	923:958	A potential biotechnological target for improving the production of biofuels is the modification of plant cell walls.
27770231	7	67	theme	walls	954:958	arg1	target					872:877	A potential biotechnological target	843:877	A potential biotechnological target for improving the production of biofuels	843:918	A potential biotechnological target for improving the production of biofuels is the modification of plant cell walls.
27770231	1	68	theme	binding	134:140	arg1	synthase					160:167	starch synthase III	153:171	starch synthase III from Arabidopsis thaliana (SBD123)	153:206	Starch binding domains of starch synthase III from Arabidopsis thaliana (SBD123) binds preferentially to cell wall polysaccharides rather than to starch in vitro.
27770231	1	68	theme	binding	134:140	arg1	domains					142:148	Starch binding domains	127:148	Starch binding domains of starch synthase III from Arabidopsis thaliana (SBD123)	127:206	Starch binding domains of starch synthase III from Arabidopsis thaliana (SBD123) binds preferentially to cell wall polysaccharides rather than to starch in vitro.
27770231	0	69	from	targeting	4:12	arg1	synthase					52:59	starch synthase III	45:63	starch synthase III	45:63	The targeting of starch binding domains from starch synthase III to the cell wall alters cell wall composition and properties.
27770231	10	70	theme	fermentable	1395:1405	arg1	sugars					1407:1412	both fermentable sugars	1390:1412	both fermentable sugars	1390:1412	The resulting transgenic plants (E8-SDB123) showed an increased biomass, higher levels of both fermentable sugars and hydrolyzed cellulose and altered cell wall properties such as higher laxity and degradability, which are valuable characteristics for the second-generation biofuels industry.
27770231	9	71	theme	starch	1261:1266	arg1	synthase					1268:1275	A. thaliana starch synthase III	1249:1279	A. thaliana starch synthase III	1249:1279	In this study, we modified the cell wall of A. thaliana by targeting the starch-binding domains of A. thaliana starch synthase III to this structure.
27770231	1	72	from	domains	142:148	arg1	SBD123					200:205	SBD123	200:205	SBD123	200:205	Starch binding domains of starch synthase III from Arabidopsis thaliana (SBD123) binds preferentially to cell wall polysaccharides rather than to starch in vitro.
27770231	1	72	from	domains	142:148	arg1	thaliana					190:197	Arabidopsis thaliana	178:197	Arabidopsis thaliana (SBD123)	178:206	Starch binding domains of starch synthase III from Arabidopsis thaliana (SBD123) binds preferentially to cell wall polysaccharides rather than to starch in vitro.
27770231	6	73	theme	plant	697:701	arg1	wall					708:711	The plant cell wall	693:711	The plant cell wall	693:711	The plant cell wall, which represents a major source of biomass for biofuel production, is composed of cellulose, hemicelluloses, pectins and lignin.
27770231	11	74	theme	domains	1757:1763	arg1	effect					1718:1723	the putative cell-wall loosening effect	1685:1723	the putative cell-wall loosening effect of the in tandem starch binding domains	1685:1763	The increased biomass and degradability phenotype of E8-SBD123 plants could be explained by the putative cell-wall loosening effect of the in tandem starch binding domains.
27770231	0	75	theme	cell	89:92	arg1	composition					99:109	cell wall composition	89:109	cell wall composition	89:109	The targeting of starch binding domains from starch synthase III to the cell wall alters cell wall composition and properties.
27770231	3	76	theme	transgenic	410:419	arg1	plants					421:426	transgenic plants	410:426	transgenic plants	410:426	Cell wall components are altered in transgenic plants.
27770231	0	77	theme	starch	17:22	arg1	domains					32:38	starch binding domains	17:38	starch binding domains from starch synthase III	17:63	The targeting of starch binding domains from starch synthase III to the cell wall alters cell wall composition and properties.
27770231	5	78	from	production	614:623	arg1	terms					642:646	terms	642:646	terms of saccharification of essential substrates	642:690	Our results suggest that the transgenic plants have an advantage for the production of bioethanol in terms of saccharification of essential substrates.
27770231	8	79	theme	biosynthetic	1049:1060	arg1	pathways					1062:1069	biosynthetic pathways	1049:1069	biosynthetic pathways	1049:1069	This modification is achieved via several strategies, including, among others, altering biosynthetic pathways and modifying the associations and structures of various cell wall components.
27770231	10	80	theme	wall	1456:1459	arg1	properties					1461:1470	cell wall properties	1451:1470	cell wall properties such as higher laxity and degradability, which are valuable characteristics for the second-generation biofuels industry	1451:1590	The resulting transgenic plants (E8-SDB123) showed an increased biomass, higher levels of both fermentable sugars and hydrolyzed cellulose and altered cell wall properties such as higher laxity and degradability, which are valuable characteristics for the second-generation biofuels industry.
27770231	10	80	theme	wall	1456:1459	arg1	higher					1480:1485	higher	1480:1485	higher	1480:1485	The resulting transgenic plants (E8-SDB123) showed an increased biomass, higher levels of both fermentable sugars and hydrolyzed cellulose and altered cell wall properties such as higher laxity and degradability, which are valuable characteristics for the second-generation biofuels industry.
27770231	0	81	theme	domains	32:38	arg1	targeting					4:12	The targeting	0:12	The targeting of starch binding domains from starch synthase III to the cell wall	0:80	The targeting of starch binding domains from starch synthase III to the cell wall alters cell wall composition and properties.
27770231	4	82	theme	present	500:506	arg1	content					532:538	present higher released glucose content	500:538	present higher released glucose content	500:538	Transgenic plants are more susceptible to digestion than wild type and present higher released glucose content.
27770231	10	83	theme	resulting	1304:1312	arg1	plants					1325:1330	transgenic plants	1314:1330	The resulting transgenic plants (E8-SDB123)	1300:1342	The resulting transgenic plants (E8-SDB123) showed an increased biomass, higher levels of both fermentable sugars and hydrolyzed cellulose and altered cell wall properties such as higher laxity and degradability, which are valuable characteristics for the second-generation biofuels industry.
27770231	10	83	theme	resulting	1304:1312	arg1	E8-SDB123					1333:1341	E8-SDB123	1333:1341	E8-SDB123	1333:1341	The resulting transgenic plants (E8-SDB123) showed an increased biomass, higher levels of both fermentable sugars and hydrolyzed cellulose and altered cell wall properties such as higher laxity and degradability, which are valuable characteristics for the second-generation biofuels industry.
27770231	0	84	theme	starch	45:50	arg1	synthase					52:59	starch synthase III	45:63	starch synthase III	45:63	The targeting of starch binding domains from starch synthase III to the cell wall alters cell wall composition and properties.
27770231	12	85	theme	biotechnological	1826:1841	arg1	tool					1843:1846	a promising biotechnological tool	1814:1846	a promising biotechnological tool for reducing of biomass recalcitrance and therefore, the need for pretreatments	1814:1926	Based on these results, our approach represents a promising biotechnological tool for reducing of biomass recalcitrance and therefore, the need for pretreatments.
27770231	3	86	theme	Cell	374:377	arg1	components					384:393	Cell wall components	374:393	Cell wall components	374:393	Cell wall components are altered in transgenic plants.
27770231	4	87	theme	released	515:522	arg1	content					532:538	present higher released glucose content	500:538	present higher released glucose content	500:538	Transgenic plants are more susceptible to digestion than wild type and present higher released glucose content.
27770231	11	88	theme	cell-wall	1698:1706	arg1	effect					1718:1723	the putative cell-wall loosening effect	1685:1723	the putative cell-wall loosening effect of the in tandem starch binding domains	1685:1763	The increased biomass and degradability phenotype of E8-SBD123 plants could be explained by the putative cell-wall loosening effect of the in tandem starch binding domains.
27770231	11	89	theme	increased	1597:1605	arg1	phenotype					1633:1641	The increased biomass and degradability phenotype	1593:1641	The increased biomass and degradability phenotype of E8-SBD123 plants	1593:1661	The increased biomass and degradability phenotype of E8-SBD123 plants could be explained by the putative cell-wall loosening effect of the in tandem starch binding domains.
27770231	2	90	theme	wild	363:366	arg1	type					368:371	wild type	363:371	wild type	363:371	Transgenic plants overexpressing SBD123 in the cell wall are larger than wild type.
27770231	7	91	theme	potential	845:853	arg1	modification					927:938	the modification	923:938	the modification of plant cell walls	923:958	A potential biotechnological target for improving the production of biofuels is the modification of plant cell walls.
27770231	7	91	theme	potential	845:853	arg1	target					872:877	A potential biotechnological target	843:877	A potential biotechnological target for improving the production of biofuels	843:918	A potential biotechnological target for improving the production of biofuels is the modification of plant cell walls.
24857869	6	0	from	effect	889:894	arg1	films					923:927	the CMC films	915:927	the CMC films	915:927	The mechanical properties of the composite films were evaluated by tensile testing, which suggested the reinforcing effect of CNFs present on the CMC films.
24857869	6	1	theme	reinforcing	877:887	arg1	effect					889:894	the reinforcing effect	873:894	the reinforcing effect of CNFs present on the CMC films	873:927	The mechanical properties of the composite films were evaluated by tensile testing, which suggested the reinforcing effect of CNFs present on the CMC films.
24857869	1	2	theme	-reinforced	146:156	arg1	films					188:192	chitin nanofiber (CNF)-reinforced carboxymethyl cellulose (CMC) films	124:192	chitin nanofiber (CNF)-reinforced carboxymethyl cellulose (CMC) films	124:192	In this study, we investigated the preparation of chitin nanofiber (CNF)-reinforced carboxymethyl cellulose (CMC) films by their electrostatic interaction.
24857869	5	3	from	presence	692:699	arg1	films					716:720	the films	712:720	the films	712:720	The presence of CNFs on the films was also confirmed by the SEM and IR measurements.
24857869	5	4	theme	SEM	748:750	arg1	measurements					759:770	the SEM and IR measurements	744:770	the SEM and IR measurements	744:770	The presence of CNFs on the films was also confirmed by the SEM and IR measurements.
24857869	2	5	theme	chitin	353:358	arg1	gels					364:367	chitin ion gels	353:367	chitin ion gels	353:367	First, CMC films and self-assembled CNF dispersions with methanol were prepared by casting technique and regeneration from chitin ion gels with an ionic liquid, respectively.
24857869	5	6	theme	CNFs	704:707	arg1	presence					692:699	The presence	688:699	The presence of CNFs on the films	688:720	The presence of CNFs on the films was also confirmed by the SEM and IR measurements.
24857869	3	7	theme	composite	540:548	arg1	films					550:554	the desired composite films	528:554	the desired composite films	528:554	Then, the CMC films were immersed in the dispersions with the different CNF contents, followed by centrifugation to obtain the desired composite films.
24857869	2	8	theme	ion	360:362	arg1	gels					364:367	chitin ion gels	353:367	chitin ion gels	353:367	First, CMC films and self-assembled CNF dispersions with methanol were prepared by casting technique and regeneration from chitin ion gels with an ionic liquid, respectively.
24857869	4	9	theme	compatibilization	659:675	arg1	procedure					677:685	the above compatibilization procedure	649:685	the above compatibilization procedure	649:685	The amounts of the absorbed CNFs on the films were calculated by the weight increases after the above compatibilization procedure.
24857869	1	10	theme	cellulose	172:180	arg1	films					188:192	chitin nanofiber (CNF)-reinforced carboxymethyl cellulose (CMC) films	124:192	chitin nanofiber (CNF)-reinforced carboxymethyl cellulose (CMC) films	124:192	In this study, we investigated the preparation of chitin nanofiber (CNF)-reinforced carboxymethyl cellulose (CMC) films by their electrostatic interaction.
24857869	2	11	theme	CNF	266:268	arg1	dispersions					270:280	self-assembled CNF dispersions	251:280	self-assembled CNF dispersions with methanol	251:294	First, CMC films and self-assembled CNF dispersions with methanol were prepared by casting technique and regeneration from chitin ion gels with an ionic liquid, respectively.
24857869	4	12	from	amounts	561:567	arg1	films					597:601	the films	593:601	the films	593:601	The amounts of the absorbed CNFs on the films were calculated by the weight increases after the above compatibilization procedure.
24857869	6	13	theme	tensile	840:846	arg1	testing					848:854	tensile testing	840:854	tensile testing	840:854	The mechanical properties of the composite films were evaluated by tensile testing, which suggested the reinforcing effect of CNFs present on the CMC films.
24857869	2	14	theme	casting	313:319	arg1	technique					321:329	casting technique	313:329	casting technique	313:329	First, CMC films and self-assembled CNF dispersions with methanol were prepared by casting technique and regeneration from chitin ion gels with an ionic liquid, respectively.
24857869	5	15	attach	presence	692:699	arg2	CNFs					704:707	CNFs	704:707	CNFs	704:707	The presence of CNFs on the films was also confirmed by the SEM and IR measurements.
24857869	5	15	attach	presence	692:699	arg1	films					716:720	the films	712:720	the films	712:720	The presence of CNFs on the films was also confirmed by the SEM and IR measurements.
24857869	1	16	theme	CMC	183:185	arg1	films					188:192	chitin nanofiber (CNF)-reinforced carboxymethyl cellulose (CMC) films	124:192	chitin nanofiber (CNF)-reinforced carboxymethyl cellulose (CMC) films	124:192	In this study, we investigated the preparation of chitin nanofiber (CNF)-reinforced carboxymethyl cellulose (CMC) films by their electrostatic interaction.
24857869	4	17	theme	weight	626:631	arg1	increases					633:641	the weight increases	622:641	the weight increases after the above compatibilization procedure	622:685	The amounts of the absorbed CNFs on the films were calculated by the weight increases after the above compatibilization procedure.
24857869	3	18	theme	CMC	415:417	arg1	films					419:423	the CMC films	411:423	the CMC films	411:423	Then, the CMC films were immersed in the dispersions with the different CNF contents, followed by centrifugation to obtain the desired composite films.
24857869	3	19	theme	desired	532:538	arg1	films					550:554	the desired composite films	528:554	the desired composite films	528:554	Then, the CMC films were immersed in the dispersions with the different CNF contents, followed by centrifugation to obtain the desired composite films.
24857869	6	20	theme	mechanical	777:786	arg1	properties					788:797	The mechanical properties	773:797	The mechanical properties of the composite films	773:820	The mechanical properties of the composite films were evaluated by tensile testing, which suggested the reinforcing effect of CNFs present on the CMC films.
24857869	1	21	theme	films	188:192	arg1	preparation					109:119	the preparation	105:119	the preparation of chitin nanofiber (CNF)-reinforced carboxymethyl cellulose (CMC) films	105:192	In this study, we investigated the preparation of chitin nanofiber (CNF)-reinforced carboxymethyl cellulose (CMC) films by their electrostatic interaction.
24857869	0	22	theme	chitin	15:20	arg1	Preparation					0:10	Preparation	0:10	Preparation of chitin	0:20	Preparation of chitin nanofiber-reinforced carboxymethyl cellulose films.
24857869	6	23	theme	CMC	919:921	arg1	films					923:927	the CMC films	915:927	the CMC films	915:927	The mechanical properties of the composite films were evaluated by tensile testing, which suggested the reinforcing effect of CNFs present on the CMC films.
24857869	1	24	theme	carboxymethyl	158:170	arg1	films					188:192	chitin nanofiber (CNF)-reinforced carboxymethyl cellulose (CMC) films	124:192	chitin nanofiber (CNF)-reinforced carboxymethyl cellulose (CMC) films	124:192	In this study, we investigated the preparation of chitin nanofiber (CNF)-reinforced carboxymethyl cellulose (CMC) films by their electrostatic interaction.
24857869	0	25	theme	cellulose	57:65	arg1	films					67:71	carboxymethyl cellulose films	43:71	carboxymethyl cellulose films	43:71	Preparation of chitin nanofiber-reinforced carboxymethyl cellulose films.
24857869	1	26	theme	chitin	124:129	arg1	nanofiber					131:139	chitin nanofiber	124:139	chitin nanofiber (CNF)-reinforced carboxymethyl cellulose (CMC) films	124:192	In this study, we investigated the preparation of chitin nanofiber (CNF)-reinforced carboxymethyl cellulose (CMC) films by their electrostatic interaction.
24857869	1	26	theme	chitin	124:129	arg1	CNF					142:144	CNF	142:144	CNF	142:144	In this study, we investigated the preparation of chitin nanofiber (CNF)-reinforced carboxymethyl cellulose (CMC) films by their electrostatic interaction.
24857869	0	27	theme	carboxymethyl	43:55	arg1	films					67:71	carboxymethyl cellulose films	43:71	carboxymethyl cellulose films	43:71	Preparation of chitin nanofiber-reinforced carboxymethyl cellulose films.
24857869	2	28	with	films	241:245	arg1	methanol					287:294	methanol	287:294	methanol	287:294	First, CMC films and self-assembled CNF dispersions with methanol were prepared by casting technique and regeneration from chitin ion gels with an ionic liquid, respectively.
24857869	3	29	with	dispersions	446:456	arg1	contents					481:488	the different CNF contents	463:488	the different CNF contents	463:488	Then, the CMC films were immersed in the dispersions with the different CNF contents, followed by centrifugation to obtain the desired composite films.
24857869	1	30	theme	electrostatic	203:215	arg1	interaction					217:227	their electrostatic interaction	197:227	their electrostatic interaction	197:227	In this study, we investigated the preparation of chitin nanofiber (CNF)-reinforced carboxymethyl cellulose (CMC) films by their electrostatic interaction.
24857869	6	31	theme	present	904:910	arg1	effect					889:894	the reinforcing effect	873:894	the reinforcing effect of CNFs present on the CMC films	873:927	The mechanical properties of the composite films were evaluated by tensile testing, which suggested the reinforcing effect of CNFs present on the CMC films.
24857869	6	32	theme	films	816:820	arg1	properties					788:797	The mechanical properties	773:797	The mechanical properties of the composite films	773:820	The mechanical properties of the composite films were evaluated by tensile testing, which suggested the reinforcing effect of CNFs present on the CMC films.
24857869	3	33	theme	different	467:475	arg1	contents					481:488	the different CNF contents	463:488	the different CNF contents	463:488	Then, the CMC films were immersed in the dispersions with the different CNF contents, followed by centrifugation to obtain the desired composite films.
24857869	5	34	theme	IR	756:757	arg1	measurements					759:770	the SEM and IR measurements	744:770	the SEM and IR measurements	744:770	The presence of CNFs on the films was also confirmed by the SEM and IR measurements.
24857869	6	35	theme	CNFs	899:902	arg1	present					904:910	CNFs present	899:910	CNFs present	899:910	The mechanical properties of the composite films were evaluated by tensile testing, which suggested the reinforcing effect of CNFs present on the CMC films.
24857869	6	36	theme	composite	806:814	arg1	films					816:820	the composite films	802:820	the composite films	802:820	The mechanical properties of the composite films were evaluated by tensile testing, which suggested the reinforcing effect of CNFs present on the CMC films.
24857869	3	37	theme	CNF	477:479	arg1	contents					481:488	the different CNF contents	463:488	the different CNF contents	463:488	Then, the CMC films were immersed in the dispersions with the different CNF contents, followed by centrifugation to obtain the desired composite films.
24857869	3	38	dep	immersed	430:437	arg1	followed					491:498	followed	491:498	followed by centrifugation to obtain the desired composite films	491:554	Then, the CMC films were immersed in the dispersions with the different CNF contents, followed by centrifugation to obtain the desired composite films.
24857869	2	39	theme	self-assembled	251:264	arg1	dispersions					270:280	self-assembled CNF dispersions	251:280	self-assembled CNF dispersions with methanol	251:294	First, CMC films and self-assembled CNF dispersions with methanol were prepared by casting technique and regeneration from chitin ion gels with an ionic liquid, respectively.
24857869	1	40	theme	nanofiber	131:139	arg1	films					188:192	chitin nanofiber (CNF)-reinforced carboxymethyl cellulose (CMC) films	124:192	chitin nanofiber (CNF)-reinforced carboxymethyl cellulose (CMC) films	124:192	In this study, we investigated the preparation of chitin nanofiber (CNF)-reinforced carboxymethyl cellulose (CMC) films by their electrostatic interaction.
24857869	2	41	with	dispersions	270:280	arg1	methanol					287:294	methanol	287:294	methanol	287:294	First, CMC films and self-assembled CNF dispersions with methanol were prepared by casting technique and regeneration from chitin ion gels with an ionic liquid, respectively.
24857869	4	42	theme	absorbed	576:583	arg1	CNFs					585:588	the absorbed CNFs	572:588	the absorbed CNFs	572:588	The amounts of the absorbed CNFs on the films were calculated by the weight increases after the above compatibilization procedure.
24857869	2	43	with	gels	364:367	arg1	liquid					383:388	liquid	383:388	liquid	383:388	First, CMC films and self-assembled CNF dispersions with methanol were prepared by casting technique and regeneration from chitin ion gels with an ionic liquid, respectively.
24857869	4	44	theme	above	653:657	arg1	procedure					677:685	the above compatibilization procedure	649:685	the above compatibilization procedure	649:685	The amounts of the absorbed CNFs on the films were calculated by the weight increases after the above compatibilization procedure.
24857869	2	45	theme	CMC	237:239	arg1	films					241:245	CMC films	237:245	CMC films	237:245	First, CMC films and self-assembled CNF dispersions with methanol were prepared by casting technique and regeneration from chitin ion gels with an ionic liquid, respectively.
24857869	4	46	theme	CNFs	585:588	arg1	CNFs					585:588	the absorbed CNFs	572:588	the absorbed CNFs	572:588	The amounts of the absorbed CNFs on the films were calculated by the weight increases after the above compatibilization procedure.
24857869	4	46	theme	CNFs	585:588	arg1	amounts					561:567	The amounts	557:567	The amounts of the absorbed CNFs on the films	557:601	The amounts of the absorbed CNFs on the films were calculated by the weight increases after the above compatibilization procedure.
27447405	0	0	theme	materials	96:104	arg1	activity					24:31	antibacterial activity	10:31	antibacterial activity	10:31	Exploring antibacterial activity and hydrolytic stability of resin dental composite restorative materials containing chitosan.
27447405	0	0	theme	materials	96:104	arg1	stability					48:56	hydrolytic stability	37:56	hydrolytic stability	37:56	Exploring antibacterial activity and hydrolytic stability of resin dental composite restorative materials containing chitosan.
27447405	5	1	theme	growth	828:833	arg1	zone					846:849	no growth inhibition zone	825:849	no growth inhibition zone	825:849	RESULTS The control and experimental flowable and microhybrid RDCRM exhibited no growth inhibition zone in the lawn growth of Lactobacilli casei.
27447405	7	2	theme	accepted	1175:1182	arg1	range					1184:1188	the ISO accepted range	1167:1188	the ISO accepted range	1167:1188	The water sorption and solubility values of control as well as experimental flowable and microhybrid RDCRM were found to be within the ISO accepted range.
27447405	6	3	theme	forming	938:944	arg1	count					951:955	colony forming unit count	931:955	colony forming unit count of Lactobacilli casei	931:977	The direct contact test revealed that colony forming unit count of Lactobacilli casei was comparable among the experimental and control RDCRM.
27447405	1	4	theme	restorative	180:190	arg1	RDCRM					203:207	RDCRM	203:207	RDCRM	203:207	BACKGROUND The development of resin dental composite restorative materials (RDCRM) with antimicrobial activity may prevent the problem of secondary caries and consequently prolong longevity of RDCRM.
27447405	1	4	theme	restorative	180:190	arg1	materials					192:200	resin dental composite restorative materials	157:200	resin dental composite restorative materials (RDCRM) with antimicrobial activity	157:236	BACKGROUND The development of resin dental composite restorative materials (RDCRM) with antimicrobial activity may prevent the problem of secondary caries and consequently prolong longevity of RDCRM.
27447405	1	5	theme	caries	275:280	arg1	problem					254:260	the problem	250:260	the problem of secondary caries	250:280	BACKGROUND The development of resin dental composite restorative materials (RDCRM) with antimicrobial activity may prevent the problem of secondary caries and consequently prolong longevity of RDCRM.
27447405	0	6	theme	restorative	84:94	arg1	materials					96:104	resin dental composite restorative materials	61:104	resin dental composite restorative materials containing chitosan	61:124	Exploring antibacterial activity and hydrolytic stability of resin dental composite restorative materials containing chitosan.
27447405	6	7	theme	casei	973:977	arg1	count					951:955	colony forming unit count	931:955	colony forming unit count of Lactobacilli casei	931:977	The direct contact test revealed that colony forming unit count of Lactobacilli casei was comparable among the experimental and control RDCRM.
27447405	1	8	with	materials	192:200	arg1	activity					229:236	antimicrobial activity	215:236	antimicrobial activity	215:236	BACKGROUND The development of resin dental composite restorative materials (RDCRM) with antimicrobial activity may prevent the problem of secondary caries and consequently prolong longevity of RDCRM.
27447405	8	9	theme	experimental	1287:1298	arg1	groups					1306:1311	different experimental RDCRM groups	1277:1311	different experimental RDCRM groups	1277:1311	There was statistically no significant difference in water sorption and solubility of different experimental RDCRM groups.
27447405	1	10	theme	materials	192:200	arg1	development					142:152	The development	138:152	The development of resin dental composite restorative materials (RDCRM) with antimicrobial activity	138:236	BACKGROUND The development of resin dental composite restorative materials (RDCRM) with antimicrobial activity may prevent the problem of secondary caries and consequently prolong longevity of RDCRM.
27447405	8	11	theme	significant	1218:1228	arg1	difference					1230:1239	no significant difference	1215:1239	no significant difference	1215:1239	There was statistically no significant difference in water sorption and solubility of different experimental RDCRM groups.
27447405	6	12	theme	Lactobacilli	960:971	arg1	casei					973:977	Lactobacilli casei	960:977	Lactobacilli casei	960:977	The direct contact test revealed that colony forming unit count of Lactobacilli casei was comparable among the experimental and control RDCRM.
27447405	3	13	dep	METHODS	435:441	arg1	investigated					595:606	investigated	595:606	was investigated using agar diffusion test and direct contact methods	591:659	METHODS The antibacterial activity of microhybrid and flowable RDCRM containing 0, 0.25, 0.5 and 1% wt/wt chitosan (CS) against Lactobacilli casei bacteria was investigated using agar diffusion test and direct contact methods.
27447405	3	14	theme	Lactobacilli	563:574	arg1	bacteria					582:589	Lactobacilli casei bacteria	563:589	Lactobacilli casei bacteria	563:589	METHODS The antibacterial activity of microhybrid and flowable RDCRM containing 0, 0.25, 0.5 and 1% wt/wt chitosan (CS) against Lactobacilli casei bacteria was investigated using agar diffusion test and direct contact methods.
27447405	7	15	theme	sorption	1046:1053	arg1	values					1070:1075	The water sorption and solubility values	1036:1075	The water sorption and solubility values of control as well as experimental flowable and microhybrid RDCRM	1036:1141	The water sorption and solubility values of control as well as experimental flowable and microhybrid RDCRM were found to be within the ISO accepted range.
27447405	3	16	theme	antibacterial	447:459	arg1	activity					461:468	The antibacterial activity	443:468	The antibacterial activity of microhybrid and flowable RDCRM containing 0, 0.25, 0.5 and 1% wt/wt chitosan (CS) against Lactobacilli casei bacteria	443:589	METHODS The antibacterial activity of microhybrid and flowable RDCRM containing 0, 0.25, 0.5 and 1% wt/wt chitosan (CS) against Lactobacilli casei bacteria was investigated using agar diffusion test and direct contact methods.
27447405	5	17	dep	RESULTS	747:753	arg1	flowable					784:791	The control and experimental flowable	755:791	The control and experimental flowable	755:791	RESULTS The control and experimental flowable and microhybrid RDCRM exhibited no growth inhibition zone in the lawn growth of Lactobacilli casei.
27447405	5	17	dep	RESULTS	747:753	arg1	RDCRM					809:813	microhybrid RDCRM	797:813	microhybrid RDCRM	797:813	RESULTS The control and experimental flowable and microhybrid RDCRM exhibited no growth inhibition zone in the lawn growth of Lactobacilli casei.
27447405	5	17	dep	RESULTS	747:753	arg1	RESULTS					747:753	RESULTS	747:753	RESULTS The control and experimental flowable and microhybrid RDCRM	747:813	RESULTS The control and experimental flowable and microhybrid RDCRM exhibited no growth inhibition zone in the lawn growth of Lactobacilli casei.
27447405	7	18	theme	ISO	1171:1173	arg1	range					1184:1188	the ISO accepted range	1167:1188	the ISO accepted range	1167:1188	The water sorption and solubility values of control as well as experimental flowable and microhybrid RDCRM were found to be within the ISO accepted range.
27447405	3	19	theme	casei	576:580	arg1	bacteria					582:589	Lactobacilli casei bacteria	563:589	Lactobacilli casei bacteria	563:589	METHODS The antibacterial activity of microhybrid and flowable RDCRM containing 0, 0.25, 0.5 and 1% wt/wt chitosan (CS) against Lactobacilli casei bacteria was investigated using agar diffusion test and direct contact methods.
27447405	9	20	contain	have	1375:1378	arg1	percentage					1334:1343	The one percentage	1326:1343	The one percentage of CS into experimental RDCRM	1326:1373	CONCLUSIONS The one percentage of CS into experimental RDCRM have no antibacterial activity against Lactobacilli casei.
27447405	9	20	contain	have	1375:1378	arg2	activity					1397:1404	no antibacterial activity	1380:1404	no antibacterial activity against Lactobacilli casei	1380:1431	CONCLUSIONS The one percentage of CS into experimental RDCRM have no antibacterial activity against Lactobacilli casei.
27447405	2	21	theme	antibacterial	357:369	arg1	activity					371:378	antibacterial activity	357:378	antibacterial activity	357:378	OBJECTIVE This study explored antibacterial activity and hydrolytic stability of RDCRM containing chitosan.
27447405	5	22	theme	microhybrid	797:807	arg1	RDCRM					809:813	microhybrid RDCRM	797:813	microhybrid RDCRM	797:813	RESULTS The control and experimental flowable and microhybrid RDCRM exhibited no growth inhibition zone in the lawn growth of Lactobacilli casei.
27447405	5	22	theme	microhybrid	797:807	arg1	RESULTS					747:753	RESULTS	747:753	RESULTS The control and experimental flowable and microhybrid RDCRM	747:813	RESULTS The control and experimental flowable and microhybrid RDCRM exhibited no growth inhibition zone in the lawn growth of Lactobacilli casei.
27447405	9	23	theme	antibacterial	1383:1395	arg1	activity					1397:1404	no antibacterial activity	1380:1404	no antibacterial activity against Lactobacilli casei	1380:1431	CONCLUSIONS The one percentage of CS into experimental RDCRM have no antibacterial activity against Lactobacilli casei.
27447405	4	24	theme	Hydrolytic	662:671	arg1	stability					673:681	Hydrolytic stability	662:681	Hydrolytic stability of experimental RDCRM	662:703	Hydrolytic stability of experimental RDCRM was evaluated using gravimetric analysis.
27447405	3	25	theme	RDCRM	498:502	arg1	activity					461:468	The antibacterial activity	443:468	The antibacterial activity of microhybrid and flowable RDCRM containing 0, 0.25, 0.5 and 1% wt/wt chitosan (CS) against Lactobacilli casei bacteria	443:589	METHODS The antibacterial activity of microhybrid and flowable RDCRM containing 0, 0.25, 0.5 and 1% wt/wt chitosan (CS) against Lactobacilli casei bacteria was investigated using agar diffusion test and direct contact methods.
27447405	5	26	theme	lawn	858:861	arg1	growth					863:868	the lawn growth	854:868	the lawn growth of Lactobacilli casei	854:890	RESULTS The control and experimental flowable and microhybrid RDCRM exhibited no growth inhibition zone in the lawn growth of Lactobacilli casei.
27447405	6	27	theme	unit	946:949	arg1	count					951:955	colony forming unit count	931:955	colony forming unit count of Lactobacilli casei	931:977	The direct contact test revealed that colony forming unit count of Lactobacilli casei was comparable among the experimental and control RDCRM.
27447405	8	28	theme	different	1277:1285	arg1	groups					1306:1311	different experimental RDCRM groups	1277:1311	different experimental RDCRM groups	1277:1311	There was statistically no significant difference in water sorption and solubility of different experimental RDCRM groups.
27447405	10	29	theme	hydrolytic	1438:1447	arg1	stability					1449:1457	The hydrolytic stability	1434:1457	The hydrolytic stability of RDCRM containing CS	1434:1480	The hydrolytic stability of RDCRM containing CS was acceptable.
27447405	10	29	theme	hydrolytic	1438:1447	arg1	acceptable					1486:1495	acceptable	1486:1495	acceptable	1486:1495	The hydrolytic stability of RDCRM containing CS was acceptable.
27447405	4	30	theme	RDCRM	699:703	arg1	stability					673:681	Hydrolytic stability	662:681	Hydrolytic stability of experimental RDCRM	662:703	Hydrolytic stability of experimental RDCRM was evaluated using gravimetric analysis.
27447405	0	31	theme	antibacterial	10:22	arg1	activity					24:31	antibacterial activity	10:31	antibacterial activity	10:31	Exploring antibacterial activity and hydrolytic stability of resin dental composite restorative materials containing chitosan.
27447405	5	32	theme	inhibition	835:844	arg1	zone					846:849	no growth inhibition zone	825:849	no growth inhibition zone	825:849	RESULTS The control and experimental flowable and microhybrid RDCRM exhibited no growth inhibition zone in the lawn growth of Lactobacilli casei.
27447405	8	33	theme	water	1244:1248	arg1	sorption					1250:1257	water sorption	1244:1257	water sorption	1244:1257	There was statistically no significant difference in water sorption and solubility of different experimental RDCRM groups.
27447405	9	34	theme	Lactobacilli	1414:1425	arg1	casei					1427:1431	Lactobacilli casei	1414:1431	Lactobacilli casei	1414:1431	CONCLUSIONS The one percentage of CS into experimental RDCRM have no antibacterial activity against Lactobacilli casei.
27447405	7	35	theme	microhybrid	1125:1135	arg1	RDCRM					1137:1141	microhybrid RDCRM	1125:1141	microhybrid RDCRM	1125:1141	The water sorption and solubility values of control as well as experimental flowable and microhybrid RDCRM were found to be within the ISO accepted range.
27447405	2	36	theme	chitosan	425:432	arg1	activity					371:378	antibacterial activity	357:378	antibacterial activity	357:378	OBJECTIVE This study explored antibacterial activity and hydrolytic stability of RDCRM containing chitosan.
27447405	2	36	theme	chitosan	425:432	arg1	stability					395:403	hydrolytic stability	384:403	hydrolytic stability	384:403	OBJECTIVE This study explored antibacterial activity and hydrolytic stability of RDCRM containing chitosan.
27447405	5	37	theme	Lactobacilli	873:884	arg1	casei					886:890	Lactobacilli casei	873:890	Lactobacilli casei	873:890	RESULTS The control and experimental flowable and microhybrid RDCRM exhibited no growth inhibition zone in the lawn growth of Lactobacilli casei.
27447405	3	38	theme	diffusion	619:627	arg1	test					629:632	agar diffusion test	614:632	agar diffusion test	614:632	METHODS The antibacterial activity of microhybrid and flowable RDCRM containing 0, 0.25, 0.5 and 1% wt/wt chitosan (CS) against Lactobacilli casei bacteria was investigated using agar diffusion test and direct contact methods.
27447405	1	39	theme	antimicrobial	215:227	arg1	activity					229:236	antimicrobial activity	215:236	antimicrobial activity	215:236	BACKGROUND The development of resin dental composite restorative materials (RDCRM) with antimicrobial activity may prevent the problem of secondary caries and consequently prolong longevity of RDCRM.
27447405	5	40	theme	casei	886:890	arg1	growth					863:868	the lawn growth	854:868	the lawn growth of Lactobacilli casei	854:890	RESULTS The control and experimental flowable and microhybrid RDCRM exhibited no growth inhibition zone in the lawn growth of Lactobacilli casei.
27447405	1	41	theme	RDCRM	320:324	arg1	longevity					307:315	longevity	307:315	longevity of RDCRM	307:324	BACKGROUND The development of resin dental composite restorative materials (RDCRM) with antimicrobial activity may prevent the problem of secondary caries and consequently prolong longevity of RDCRM.
27447405	5	42	theme	control	759:765	arg1	flowable					784:791	The control and experimental flowable	755:791	The control and experimental flowable	755:791	RESULTS The control and experimental flowable and microhybrid RDCRM exhibited no growth inhibition zone in the lawn growth of Lactobacilli casei.
27447405	5	42	theme	control	759:765	arg1	RESULTS					747:753	RESULTS	747:753	RESULTS The control and experimental flowable and microhybrid RDCRM	747:813	RESULTS The control and experimental flowable and microhybrid RDCRM exhibited no growth inhibition zone in the lawn growth of Lactobacilli casei.
27447405	6	43	theme	colony	931:936	arg1	count					951:955	colony forming unit count	931:955	colony forming unit count of Lactobacilli casei	931:977	The direct contact test revealed that colony forming unit count of Lactobacilli casei was comparable among the experimental and control RDCRM.
27447405	5	44	theme	experimental	771:782	arg1	flowable					784:791	The control and experimental flowable	755:791	The control and experimental flowable	755:791	RESULTS The control and experimental flowable and microhybrid RDCRM exhibited no growth inhibition zone in the lawn growth of Lactobacilli casei.
27447405	5	44	theme	experimental	771:782	arg1	RESULTS					747:753	RESULTS	747:753	RESULTS The control and experimental flowable and microhybrid RDCRM	747:813	RESULTS The control and experimental flowable and microhybrid RDCRM exhibited no growth inhibition zone in the lawn growth of Lactobacilli casei.
27447405	1	45	theme	dental	163:168	arg1	RDCRM					203:207	RDCRM	203:207	RDCRM	203:207	BACKGROUND The development of resin dental composite restorative materials (RDCRM) with antimicrobial activity may prevent the problem of secondary caries and consequently prolong longevity of RDCRM.
27447405	1	45	theme	dental	163:168	arg1	materials					192:200	resin dental composite restorative materials	157:200	resin dental composite restorative materials (RDCRM) with antimicrobial activity	157:236	BACKGROUND The development of resin dental composite restorative materials (RDCRM) with antimicrobial activity may prevent the problem of secondary caries and consequently prolong longevity of RDCRM.
27447405	3	46	theme	wt/wt	535:539	arg1	chitosan					541:548	0, 0.25, 0.5 and 1% wt/wt chitosan	515:548	0, 0.25, 0.5 and 1% wt/wt chitosan (CS)	515:553	METHODS The antibacterial activity of microhybrid and flowable RDCRM containing 0, 0.25, 0.5 and 1% wt/wt chitosan (CS) against Lactobacilli casei bacteria was investigated using agar diffusion test and direct contact methods.
27447405	3	46	theme	wt/wt	535:539	arg1	CS					551:552	CS	551:552	CS	551:552	METHODS The antibacterial activity of microhybrid and flowable RDCRM containing 0, 0.25, 0.5 and 1% wt/wt chitosan (CS) against Lactobacilli casei bacteria was investigated using agar diffusion test and direct contact methods.
27447405	3	47	theme	%	533:533	arg1	chitosan					541:548	0, 0.25, 0.5 and 1% wt/wt chitosan	515:548	0, 0.25, 0.5 and 1% wt/wt chitosan (CS)	515:553	METHODS The antibacterial activity of microhybrid and flowable RDCRM containing 0, 0.25, 0.5 and 1% wt/wt chitosan (CS) against Lactobacilli casei bacteria was investigated using agar diffusion test and direct contact methods.
27447405	3	47	theme	%	533:533	arg1	CS					551:552	CS	551:552	CS	551:552	METHODS The antibacterial activity of microhybrid and flowable RDCRM containing 0, 0.25, 0.5 and 1% wt/wt chitosan (CS) against Lactobacilli casei bacteria was investigated using agar diffusion test and direct contact methods.
27447405	3	48	theme	test	629:632	arg1	methods					653:659	agar diffusion test and direct contact methods	614:659	agar diffusion test and direct contact methods	614:659	METHODS The antibacterial activity of microhybrid and flowable RDCRM containing 0, 0.25, 0.5 and 1% wt/wt chitosan (CS) against Lactobacilli casei bacteria was investigated using agar diffusion test and direct contact methods.
27447405	3	49	theme	flowable	489:496	arg1	RDCRM					498:502	microhybrid and flowable RDCRM	473:502	microhybrid and flowable RDCRM containing 0, 0.25, 0.5 and 1% wt/wt chitosan (CS)	473:553	METHODS The antibacterial activity of microhybrid and flowable RDCRM containing 0, 0.25, 0.5 and 1% wt/wt chitosan (CS) against Lactobacilli casei bacteria was investigated using agar diffusion test and direct contact methods.
27447405	4	50	theme	experimental	686:697	arg1	RDCRM					699:703	experimental RDCRM	686:703	experimental RDCRM	686:703	Hydrolytic stability of experimental RDCRM was evaluated using gravimetric analysis.
27447405	0	51	theme	hydrolytic	37:46	arg1	stability					48:56	hydrolytic stability	37:56	hydrolytic stability	37:56	Exploring antibacterial activity and hydrolytic stability of resin dental composite restorative materials containing chitosan.
27447405	3	52	theme	microhybrid	473:483	arg1	RDCRM					498:502	microhybrid and flowable RDCRM	473:502	microhybrid and flowable RDCRM containing 0, 0.25, 0.5 and 1% wt/wt chitosan (CS)	473:553	METHODS The antibacterial activity of microhybrid and flowable RDCRM containing 0, 0.25, 0.5 and 1% wt/wt chitosan (CS) against Lactobacilli casei bacteria was investigated using agar diffusion test and direct contact methods.
27447405	7	53	theme	control	1080:1086	arg1	values					1070:1075	The water sorption and solubility values	1036:1075	The water sorption and solubility values of control as well as experimental flowable and microhybrid RDCRM	1036:1141	The water sorption and solubility values of control as well as experimental flowable and microhybrid RDCRM were found to be within the ISO accepted range.
27447405	0	54	theme	resin	61:65	arg1	materials					96:104	resin dental composite restorative materials	61:104	resin dental composite restorative materials containing chitosan	61:124	Exploring antibacterial activity and hydrolytic stability of resin dental composite restorative materials containing chitosan.
27447405	7	55	theme	experimental	1099:1110	arg1	flowable					1112:1119	experimental flowable	1099:1119	experimental flowable	1099:1119	The water sorption and solubility values of control as well as experimental flowable and microhybrid RDCRM were found to be within the ISO accepted range.
27447405	6	56	dep	experimental	1004:1015	arg1	RDCRM					1029:1033	RDCRM	1029:1033	RDCRM	1029:1033	The direct contact test revealed that colony forming unit count of Lactobacilli casei was comparable among the experimental and control RDCRM.
27447405	3	57	theme	agar	614:617	arg1	test					629:632	agar diffusion test	614:632	agar diffusion test	614:632	METHODS The antibacterial activity of microhybrid and flowable RDCRM containing 0, 0.25, 0.5 and 1% wt/wt chitosan (CS) against Lactobacilli casei bacteria was investigated using agar diffusion test and direct contact methods.
27447405	9	58	theme	experimental	1356:1367	arg1	RDCRM					1369:1373	experimental RDCRM	1356:1373	experimental RDCRM	1356:1373	CONCLUSIONS The one percentage of CS into experimental RDCRM have no antibacterial activity against Lactobacilli casei.
27447405	2	59	theme	hydrolytic	384:393	arg1	stability					395:403	hydrolytic stability	384:403	hydrolytic stability	384:403	OBJECTIVE This study explored antibacterial activity and hydrolytic stability of RDCRM containing chitosan.
27447405	2	60	theme	RDCRM	408:412	arg1	chitosan					425:432	RDCRM containing chitosan	408:432	RDCRM containing chitosan	408:432	OBJECTIVE This study explored antibacterial activity and hydrolytic stability of RDCRM containing chitosan.
27447405	7	61	theme	flowable	1112:1119	arg1	values					1070:1075	The water sorption and solubility values	1036:1075	The water sorption and solubility values of control as well as experimental flowable and microhybrid RDCRM	1036:1141	The water sorption and solubility values of control as well as experimental flowable and microhybrid RDCRM were found to be within the ISO accepted range.
27447405	6	62	theme	contact	904:910	arg1	test					912:915	The direct contact test	893:915	The direct contact test	893:915	The direct contact test revealed that colony forming unit count of Lactobacilli casei was comparable among the experimental and control RDCRM.
27447405	1	63	theme	composite	170:178	arg1	RDCRM					203:207	RDCRM	203:207	RDCRM	203:207	BACKGROUND The development of resin dental composite restorative materials (RDCRM) with antimicrobial activity may prevent the problem of secondary caries and consequently prolong longevity of RDCRM.
27447405	1	63	theme	composite	170:178	arg1	materials					192:200	resin dental composite restorative materials	157:200	resin dental composite restorative materials (RDCRM) with antimicrobial activity	157:236	BACKGROUND The development of resin dental composite restorative materials (RDCRM) with antimicrobial activity may prevent the problem of secondary caries and consequently prolong longevity of RDCRM.
27447405	0	64	contain	containing	106:115	arg2	chitosan					117:124	chitosan	117:124	chitosan	117:124	Exploring antibacterial activity and hydrolytic stability of resin dental composite restorative materials containing chitosan.
27447405	0	64	contain	containing	106:115	arg1	materials					96:104	resin dental composite restorative materials	61:104	resin dental composite restorative materials containing chitosan	61:124	Exploring antibacterial activity and hydrolytic stability of resin dental composite restorative materials containing chitosan.
27447405	2	65	dep	OBJECTIVE	327:335	arg1	explored					348:355	explored	348:355	explored antibacterial activity and hydrolytic stability of RDCRM containing chitosan	348:432	OBJECTIVE This study explored antibacterial activity and hydrolytic stability of RDCRM containing chitosan.
27447405	10	66	contain	containing	1468:1477	arg2	CS					1479:1480	CS	1479:1480	CS	1479:1480	The hydrolytic stability of RDCRM containing CS was acceptable.
27447405	10	66	contain	containing	1468:1477	arg1	RDCRM					1462:1466	RDCRM	1462:1466	RDCRM containing CS	1462:1480	The hydrolytic stability of RDCRM containing CS was acceptable.
27447405	6	67	theme	direct	897:902	arg1	test					912:915	The direct contact test	893:915	The direct contact test	893:915	The direct contact test revealed that colony forming unit count of Lactobacilli casei was comparable among the experimental and control RDCRM.
27447405	4	68	theme	gravimetric	725:735	arg1	analysis					737:744	gravimetric analysis	725:744	gravimetric analysis	725:744	Hydrolytic stability of experimental RDCRM was evaluated using gravimetric analysis.
27447405	9	69	dep	CONCLUSIONS	1314:1324	arg1	have					1375:1378	have	1375:1378	have no antibacterial activity against Lactobacilli casei	1375:1431	CONCLUSIONS The one percentage of CS into experimental RDCRM have no antibacterial activity against Lactobacilli casei.
27447405	3	70	contain	containing	504:513	arg1	RDCRM					498:502	microhybrid and flowable RDCRM	473:502	microhybrid and flowable RDCRM containing 0, 0.25, 0.5 and 1% wt/wt chitosan (CS)	473:553	METHODS The antibacterial activity of microhybrid and flowable RDCRM containing 0, 0.25, 0.5 and 1% wt/wt chitosan (CS) against Lactobacilli casei bacteria was investigated using agar diffusion test and direct contact methods.
27447405	3	70	contain	containing	504:513	arg2	CS					551:552	CS	551:552	CS	551:552	METHODS The antibacterial activity of microhybrid and flowable RDCRM containing 0, 0.25, 0.5 and 1% wt/wt chitosan (CS) against Lactobacilli casei bacteria was investigated using agar diffusion test and direct contact methods.
27447405	3	70	contain	containing	504:513	arg2	chitosan					541:548	0, 0.25, 0.5 and 1% wt/wt chitosan	515:548	0, 0.25, 0.5 and 1% wt/wt chitosan (CS)	515:553	METHODS The antibacterial activity of microhybrid and flowable RDCRM containing 0, 0.25, 0.5 and 1% wt/wt chitosan (CS) against Lactobacilli casei bacteria was investigated using agar diffusion test and direct contact methods.
27447405	10	71	theme	RDCRM	1462:1466	arg1	stability					1449:1457	The hydrolytic stability	1434:1457	The hydrolytic stability of RDCRM containing CS	1434:1480	The hydrolytic stability of RDCRM containing CS was acceptable.
27447405	10	71	theme	RDCRM	1462:1466	arg1	acceptable					1486:1495	acceptable	1486:1495	acceptable	1486:1495	The hydrolytic stability of RDCRM containing CS was acceptable.
27447405	1	72	theme	resin	157:161	arg1	RDCRM					203:207	RDCRM	203:207	RDCRM	203:207	BACKGROUND The development of resin dental composite restorative materials (RDCRM) with antimicrobial activity may prevent the problem of secondary caries and consequently prolong longevity of RDCRM.
27447405	1	72	theme	resin	157:161	arg1	materials					192:200	resin dental composite restorative materials	157:200	resin dental composite restorative materials (RDCRM) with antimicrobial activity	157:236	BACKGROUND The development of resin dental composite restorative materials (RDCRM) with antimicrobial activity may prevent the problem of secondary caries and consequently prolong longevity of RDCRM.
27447405	8	73	theme	RDCRM	1300:1304	arg1	groups					1306:1311	different experimental RDCRM groups	1277:1311	different experimental RDCRM groups	1277:1311	There was statistically no significant difference in water sorption and solubility of different experimental RDCRM groups.
27447405	0	74	theme	composite	74:82	arg1	materials					96:104	resin dental composite restorative materials	61:104	resin dental composite restorative materials containing chitosan	61:124	Exploring antibacterial activity and hydrolytic stability of resin dental composite restorative materials containing chitosan.
27447405	9	75	theme	CS	1348:1349	arg1	percentage					1334:1343	The one percentage	1326:1343	The one percentage of CS into experimental RDCRM	1326:1373	CONCLUSIONS The one percentage of CS into experimental RDCRM have no antibacterial activity against Lactobacilli casei.
27447405	3	76	theme	direct	638:643	arg1	contact					645:651	direct contact	638:651	direct contact	638:651	METHODS The antibacterial activity of microhybrid and flowable RDCRM containing 0, 0.25, 0.5 and 1% wt/wt chitosan (CS) against Lactobacilli casei bacteria was investigated using agar diffusion test and direct contact methods.
27447405	7	77	theme	solubility	1059:1068	arg1	values					1070:1075	The water sorption and solubility values	1036:1075	The water sorption and solubility values of control as well as experimental flowable and microhybrid RDCRM	1036:1141	The water sorption and solubility values of control as well as experimental flowable and microhybrid RDCRM were found to be within the ISO accepted range.
27447405	2	78	theme	containing	414:423	arg1	chitosan					425:432	RDCRM containing chitosan	408:432	RDCRM containing chitosan	408:432	OBJECTIVE This study explored antibacterial activity and hydrolytic stability of RDCRM containing chitosan.
27447405	8	79	theme	groups	1306:1311	arg1	solubility					1263:1272	solubility	1263:1272	solubility	1263:1272	There was statistically no significant difference in water sorption and solubility of different experimental RDCRM groups.
27447405	8	79	theme	groups	1306:1311	arg1	sorption					1250:1257	water sorption	1244:1257	water sorption	1244:1257	There was statistically no significant difference in water sorption and solubility of different experimental RDCRM groups.
27447405	0	80	theme	dental	67:72	arg1	materials					96:104	resin dental composite restorative materials	61:104	resin dental composite restorative materials containing chitosan	61:124	Exploring antibacterial activity and hydrolytic stability of resin dental composite restorative materials containing chitosan.
27447405	3	81	theme	contact	645:651	arg1	methods					653:659	agar diffusion test and direct contact methods	614:659	agar diffusion test and direct contact methods	614:659	METHODS The antibacterial activity of microhybrid and flowable RDCRM containing 0, 0.25, 0.5 and 1% wt/wt chitosan (CS) against Lactobacilli casei bacteria was investigated using agar diffusion test and direct contact methods.
27447405	7	82	theme	water	1040:1044	arg1	sorption					1046:1053	water sorption	1040:1053	water sorption	1040:1053	The water sorption and solubility values of control as well as experimental flowable and microhybrid RDCRM were found to be within the ISO accepted range.
27447405	1	83	dep	BACKGROUND	127:136	arg1	prolong					299:305	prolong	299:305	prolong longevity of RDCRM	299:324	BACKGROUND The development of resin dental composite restorative materials (RDCRM) with antimicrobial activity may prevent the problem of secondary caries and consequently prolong longevity of RDCRM.
27447405	1	83	dep	BACKGROUND	127:136	arg1	prevent					242:248	prevent	242:248	prevent the problem of secondary caries	242:280	BACKGROUND The development of resin dental composite restorative materials (RDCRM) with antimicrobial activity may prevent the problem of secondary caries and consequently prolong longevity of RDCRM.
27447405	1	84	theme	secondary	265:273	arg1	caries					275:280	secondary caries	265:280	secondary caries	265:280	BACKGROUND The development of resin dental composite restorative materials (RDCRM) with antimicrobial activity may prevent the problem of secondary caries and consequently prolong longevity of RDCRM.
28804800	9	0	with	variation	1579:1587	arg1	parameters					1641:1650	the physico-chemical parameters	1620:1650	the physico-chemical parameters of the process	1620:1665	These behaviors are explained by the variation of the membrane thickness with the physico-chemical parameters of the process.
28804800	4	1	theme	thorough	564:571	arg1	study					573:577	This thorough study	559:577	This thorough study	559:577	This thorough study provides new insights into the mechanics of these weakly cohesive membranes.
28804800	8	2	theme	yield	1474:1478	arg1	terms					1438:1442	terms	1438:1442	terms of elasticity, plasticity and yield stress whose magnitudes can be controlled by the composition	1438:1539	Finally, we show that the design of microcapsules can be 'engineered' in an extensive way since they present a wealth of interfacial rheological properties in terms of elasticity, plasticity and yield stress whose magnitudes can be controlled by the composition.
28804800	8	3	dep	elasticity	1447:1456	arg1	stress					1480:1485	stress	1480:1485	stress	1480:1485	Finally, we show that the design of microcapsules can be 'engineered' in an extensive way since they present a wealth of interfacial rheological properties in terms of elasticity, plasticity and yield stress whose magnitudes can be controlled by the composition.
28804800	4	4	theme	new	588:590	arg1	insights					592:599	new insights	588:599	new insights into the mechanics of these weakly cohesive membranes	588:653	This thorough study provides new insights into the mechanics of these weakly cohesive membranes.
28804800	4	5	theme	membranes	645:653	arg1	mechanics					610:618	the mechanics	606:618	the mechanics of these weakly cohesive membranes	606:653	This thorough study provides new insights into the mechanics of these weakly cohesive membranes.
28804800	3	6	theme	article	403:409	arg1	objective					385:393	The objective	381:393	The objective of this article	381:409	The objective of this article is to characterize the interfacial rheological properties of self-assembling biopolymer microcapsules that were obtained in one single facile step.
28804800	7	7	theme	flow	1165:1168	arg1	chamber					1170:1176	an extensional flow chamber	1150:1176	an extensional flow chamber which extends the relevance and convenience of the hydrodynamic method to weakly cohesive membranes	1150:1276	Secondly, microcapsules were mechanically characterized by stretching them up to break-up in an extensional flow chamber which extends the relevance and convenience of the hydrodynamic method to weakly cohesive membranes.
28804800	9	8	theme	physico-chemical	1624:1639	arg1	parameters					1641:1650	the physico-chemical parameters	1620:1650	the physico-chemical parameters of the process	1620:1665	These behaviors are explained by the variation of the membrane thickness with the physico-chemical parameters of the process.
28804800	8	9	theme	interfacial	1400:1410	arg1	properties					1424:1433	interfacial rheological properties	1400:1433	interfacial rheological properties in terms of elasticity, plasticity and yield stress whose magnitudes can be controlled by the composition	1400:1539	Finally, we show that the design of microcapsules can be 'engineered' in an extensive way since they present a wealth of interfacial rheological properties in terms of elasticity, plasticity and yield stress whose magnitudes can be controlled by the composition.
28804800	5	10	theme	water-in-oil	680:691	arg1	microcapsules					693:705	water-in-oil microcapsules	680:705	water-in-oil microcapsules	680:705	Firstly, suspensions of water-in-oil microcapsules were formed in microfluidic junctions by self-assembly of two oppositely charged polyelectrolytes, namely chitosan (water soluble) and phosphatidic fatty acid (oil soluble).
28804800	7	11	dep	relevance	1196:1204	arg1	the					1192:1194	the	1192:1194	the	1192:1194	Secondly, microcapsules were mechanically characterized by stretching them up to break-up in an extensional flow chamber which extends the relevance and convenience of the hydrodynamic method to weakly cohesive membranes.
28804800	5	12	theme	microcapsules	693:705	arg1	suspensions					665:675	suspensions	665:675	suspensions of water-in-oil microcapsules	665:705	Firstly, suspensions of water-in-oil microcapsules were formed in microfluidic junctions by self-assembly of two oppositely charged polyelectrolytes, namely chitosan (water soluble) and phosphatidic fatty acid (oil soluble).
28804800	1	13	theme	mechanical	91:100	arg1	properties					102:111	the mechanical properties	87:111	Tuning the mechanical properties of microcapsules through a cost-efficient route of fabrication	80:174	Tuning the mechanical properties of microcapsules through a cost-efficient route of fabrication is still a challenge.
28804800	5	14	theme	soluble	871:877	arg1	oil					867:869	oil soluble	867:877	oil soluble	867:877	Firstly, suspensions of water-in-oil microcapsules were formed in microfluidic junctions by self-assembly of two oppositely charged polyelectrolytes, namely chitosan (water soluble) and phosphatidic fatty acid (oil soluble).
28804800	5	14	theme	soluble	871:877	arg1	acid					861:864	phosphatidic fatty acid	842:864	phosphatidic fatty acid (oil soluble)	842:878	Firstly, suspensions of water-in-oil microcapsules were formed in microfluidic junctions by self-assembly of two oppositely charged polyelectrolytes, namely chitosan (water soluble) and phosphatidic fatty acid (oil soluble).
28804800	7	15	theme	extensional	1153:1163	arg1	chamber					1170:1176	an extensional flow chamber	1150:1176	an extensional flow chamber which extends the relevance and convenience of the hydrodynamic method to weakly cohesive membranes	1150:1276	Secondly, microcapsules were mechanically characterized by stretching them up to break-up in an extensional flow chamber which extends the relevance and convenience of the hydrodynamic method to weakly cohesive membranes.
28804800	7	16	theme	cohesive	1259:1266	arg1	membranes					1268:1276	weakly cohesive membranes	1252:1276	weakly cohesive membranes	1252:1276	Secondly, microcapsules were mechanically characterized by stretching them up to break-up in an extensional flow chamber which extends the relevance and convenience of the hydrodynamic method to weakly cohesive membranes.
28804800	0	17	theme	Interfacial	0:10	arg1	properties					24:33	Interfacial rheological properties	0:33	Interfacial rheological properties of self-assembling biopolymer microcapsules	0:77	Interfacial rheological properties of self-assembling biopolymer microcapsules.
28804800	8	18	from	terms	1438:1442	arg1	wealth					1390:1395	a wealth	1388:1395	a wealth of interfacial rheological properties in terms of elasticity, plasticity and yield stress whose magnitudes can be controlled by the composition	1388:1539	Finally, we show that the design of microcapsules can be 'engineered' in an extensive way since they present a wealth of interfacial rheological properties in terms of elasticity, plasticity and yield stress whose magnitudes can be controlled by the composition.
28804800	9	19	theme	membrane	1596:1603	arg1	thickness					1605:1613	the membrane thickness	1592:1613	the membrane thickness	1592:1613	These behaviors are explained by the variation of the membrane thickness with the physico-chemical parameters of the process.
28804800	0	20	theme	rheological	12:22	arg1	properties					24:33	Interfacial rheological properties	0:33	Interfacial rheological properties of self-assembling biopolymer microcapsules	0:77	Interfacial rheological properties of self-assembling biopolymer microcapsules.
28804800	5	21	dep	chitosan	813:820	arg1	soluble					829:835	soluble	829:835	soluble	829:835	Firstly, suspensions of water-in-oil microcapsules were formed in microfluidic junctions by self-assembly of two oppositely charged polyelectrolytes, namely chitosan (water soluble) and phosphatidic fatty acid (oil soluble).
28804800	2	22	theme	numerous	365:372	arg1	steps					374:378	numerous steps	365:378	numerous steps	365:378	The traditional method of layer-by-layer assembly of microcapsules allows building a tailored composite multi-layer membrane but is technically complex as it requires numerous steps.
28804800	9	23	theme	process	1659:1665	arg1	parameters					1641:1650	the physico-chemical parameters	1620:1650	the physico-chemical parameters of the process	1620:1665	These behaviors are explained by the variation of the membrane thickness with the physico-chemical parameters of the process.
28804800	3	24	theme	biopolymer	488:497	arg1	microcapsules					499:511	self-assembling biopolymer microcapsules	472:511	self-assembling biopolymer microcapsules that were obtained in one single facile step	472:556	The objective of this article is to characterize the interfacial rheological properties of self-assembling biopolymer microcapsules that were obtained in one single facile step.
28804800	3	25	theme	interfacial	434:444	arg1	properties					458:467	the interfacial rheological properties	430:467	the interfacial rheological properties of self-assembling biopolymer microcapsules that were obtained in one single facile step	430:556	The objective of this article is to characterize the interfacial rheological properties of self-assembling biopolymer microcapsules that were obtained in one single facile step.
28804800	7	26	from	break-up	1138:1145	arg1	chamber					1170:1176	an extensional flow chamber	1150:1176	an extensional flow chamber which extends the relevance and convenience of the hydrodynamic method to weakly cohesive membranes	1150:1276	Secondly, microcapsules were mechanically characterized by stretching them up to break-up in an extensional flow chamber which extends the relevance and convenience of the hydrodynamic method to weakly cohesive membranes.
28804800	5	27	theme	microfluidic	722:733	arg1	junctions					735:743	microfluidic junctions	722:743	microfluidic junctions	722:743	Firstly, suspensions of water-in-oil microcapsules were formed in microfluidic junctions by self-assembly of two oppositely charged polyelectrolytes, namely chitosan (water soluble) and phosphatidic fatty acid (oil soluble).
28804800	1	28	theme	microcapsules	116:128	arg1	properties					102:111	the mechanical properties	87:111	Tuning the mechanical properties of microcapsules through a cost-efficient route of fabrication	80:174	Tuning the mechanical properties of microcapsules through a cost-efficient route of fabrication is still a challenge.
28804800	3	29	theme	microcapsules	499:511	arg1	properties					458:467	the interfacial rheological properties	430:467	the interfacial rheological properties of self-assembling biopolymer microcapsules that were obtained in one single facile step	430:556	The objective of this article is to characterize the interfacial rheological properties of self-assembling biopolymer microcapsules that were obtained in one single facile step.
28804800	6	30	theme	single	1016:1021	arg1	step					1023:1026	a single step	1014:1026	a single step	1014:1026	In this way, composite membranes of tunable thickness (between 40 and 900 nm measured by AFM) were formed at water/oil interfaces in a single step by changing the composition.
28804800	7	31	dep	break-up	1138:1145	arg1	up					1132:1133	up	1132:1133	up	1132:1133	Secondly, microcapsules were mechanically characterized by stretching them up to break-up in an extensional flow chamber which extends the relevance and convenience of the hydrodynamic method to weakly cohesive membranes.
28804800	8	32	theme	properties	1424:1433	arg1	wealth					1390:1395	a wealth	1388:1395	a wealth of interfacial rheological properties in terms of elasticity, plasticity and yield stress whose magnitudes can be controlled by the composition	1388:1539	Finally, we show that the design of microcapsules can be 'engineered' in an extensive way since they present a wealth of interfacial rheological properties in terms of elasticity, plasticity and yield stress whose magnitudes can be controlled by the composition.
28804800	8	33	theme	microcapsules	1315:1327	arg1	design					1305:1310	the design	1301:1310	the design of microcapsules	1301:1327	Finally, we show that the design of microcapsules can be 'engineered' in an extensive way since they present a wealth of interfacial rheological properties in terms of elasticity, plasticity and yield stress whose magnitudes can be controlled by the composition.
28804800	8	33	theme	microcapsules	1315:1327	arg1	engineered					1337:1346	engineered	1337:1346	engineered	1337:1346	Finally, we show that the design of microcapsules can be 'engineered' in an extensive way since they present a wealth of interfacial rheological properties in terms of elasticity, plasticity and yield stress whose magnitudes can be controlled by the composition.
28804800	4	34	theme	cohesive	636:643	arg1	membranes					645:653	these weakly cohesive membranes	623:653	these weakly cohesive membranes	623:653	This thorough study provides new insights into the mechanics of these weakly cohesive membranes.
28804800	8	35	theme	rheological	1412:1422	arg1	properties					1424:1433	interfacial rheological properties	1400:1433	interfacial rheological properties in terms of elasticity, plasticity and yield stress whose magnitudes can be controlled by the composition	1400:1539	Finally, we show that the design of microcapsules can be 'engineered' in an extensive way since they present a wealth of interfacial rheological properties in terms of elasticity, plasticity and yield stress whose magnitudes can be controlled by the composition.
28804800	3	36	theme	single	539:544	arg1	step					553:556	one single facile step	535:556	one single facile step	535:556	The objective of this article is to characterize the interfacial rheological properties of self-assembling biopolymer microcapsules that were obtained in one single facile step.
28804800	0	37	theme	biopolymer	54:63	arg1	microcapsules					65:77	self-assembling biopolymer microcapsules	38:77	self-assembling biopolymer microcapsules	38:77	Interfacial rheological properties of self-assembling biopolymer microcapsules.
28804800	9	38	theme	thickness	1605:1613	arg1	variation					1579:1587	the variation	1575:1587	the variation of the membrane thickness with the physico-chemical parameters of the process	1575:1665	These behaviors are explained by the variation of the membrane thickness with the physico-chemical parameters of the process.
28804800	2	39	theme	traditional	202:212	arg1	complex					342:348	complex	342:348	complex	342:348	The traditional method of layer-by-layer assembly of microcapsules allows building a tailored composite multi-layer membrane but is technically complex as it requires numerous steps.
28804800	2	39	theme	traditional	202:212	arg1	method					214:219	The traditional method	198:219	The traditional method of layer-by-layer assembly of microcapsules	198:263	The traditional method of layer-by-layer assembly of microcapsules allows building a tailored composite multi-layer membrane but is technically complex as it requires numerous steps.
28804800	0	40	theme	self-assembling	38:52	arg1	microcapsules					65:77	self-assembling biopolymer microcapsules	38:77	self-assembling biopolymer microcapsules	38:77	Interfacial rheological properties of self-assembling biopolymer microcapsules.
28804800	3	41	theme	self-assembling	472:486	arg1	microcapsules					499:511	self-assembling biopolymer microcapsules	472:511	self-assembling biopolymer microcapsules that were obtained in one single facile step	472:556	The objective of this article is to characterize the interfacial rheological properties of self-assembling biopolymer microcapsules that were obtained in one single facile step.
28804800	3	42	theme	rheological	446:456	arg1	properties					458:467	the interfacial rheological properties	430:467	the interfacial rheological properties of self-assembling biopolymer microcapsules that were obtained in one single facile step	430:556	The objective of this article is to characterize the interfacial rheological properties of self-assembling biopolymer microcapsules that were obtained in one single facile step.
28804800	6	43	theme	water/oil	990:998	arg1	interfaces					1000:1009	water/oil interfaces	990:1009	water/oil interfaces in a single step	990:1026	In this way, composite membranes of tunable thickness (between 40 and 900 nm measured by AFM) were formed at water/oil interfaces in a single step by changing the composition.
28804800	6	44	theme	thickness	925:933	arg1	membranes					904:912	composite membranes	894:912	composite membranes of tunable thickness (between 40 and 900 nm measured by AFM)	894:973	In this way, composite membranes of tunable thickness (between 40 and 900 nm measured by AFM) were formed at water/oil interfaces in a single step by changing the composition.
28804800	8	45	theme	elasticity	1447:1456	arg1	terms					1438:1442	terms	1438:1442	terms of elasticity, plasticity and yield stress whose magnitudes can be controlled by the composition	1438:1539	Finally, we show that the design of microcapsules can be 'engineered' in an extensive way since they present a wealth of interfacial rheological properties in terms of elasticity, plasticity and yield stress whose magnitudes can be controlled by the composition.
28804800	6	46	from	interfaces	1000:1009	arg1	step					1023:1026	a single step	1014:1026	a single step	1014:1026	In this way, composite membranes of tunable thickness (between 40 and 900 nm measured by AFM) were formed at water/oil interfaces in a single step by changing the composition.
28804800	1	47	theme	cost-efficient	140:153	arg1	route					155:159	a cost-efficient route	138:159	a cost-efficient route of fabrication	138:174	Tuning the mechanical properties of microcapsules through a cost-efficient route of fabrication is still a challenge.
28804800	0	48	theme	microcapsules	65:77	arg1	properties					24:33	Interfacial rheological properties	0:33	Interfacial rheological properties of self-assembling biopolymer microcapsules	0:77	Interfacial rheological properties of self-assembling biopolymer microcapsules.
28804800	6	49	theme	tunable	917:923	arg1	thickness					925:933	tunable thickness	917:933	tunable thickness	917:933	In this way, composite membranes of tunable thickness (between 40 and 900 nm measured by AFM) were formed at water/oil interfaces in a single step by changing the composition.
28804800	2	50	theme	microcapsules	251:263	arg1	assembly					239:246	layer-by-layer assembly	224:246	layer-by-layer assembly of microcapsules	224:263	The traditional method of layer-by-layer assembly of microcapsules allows building a tailored composite multi-layer membrane but is technically complex as it requires numerous steps.
28804800	8	51	from	wealth	1390:1395	arg1	terms					1438:1442	terms	1438:1442	terms of elasticity, plasticity and yield stress whose magnitudes can be controlled by the composition	1438:1539	Finally, we show that the design of microcapsules can be 'engineered' in an extensive way since they present a wealth of interfacial rheological properties in terms of elasticity, plasticity and yield stress whose magnitudes can be controlled by the composition.
28804800	5	52	theme	charged	780:786	arg1	acid					861:864	phosphatidic fatty acid	842:864	phosphatidic fatty acid (oil soluble)	842:878	Firstly, suspensions of water-in-oil microcapsules were formed in microfluidic junctions by self-assembly of two oppositely charged polyelectrolytes, namely chitosan (water soluble) and phosphatidic fatty acid (oil soluble).
28804800	5	52	theme	charged	780:786	arg1	polyelectrolytes					788:803	two oppositely charged polyelectrolytes	765:803	two oppositely charged polyelectrolytes	765:803	Firstly, suspensions of water-in-oil microcapsules were formed in microfluidic junctions by self-assembly of two oppositely charged polyelectrolytes, namely chitosan (water soluble) and phosphatidic fatty acid (oil soluble).
28804800	5	52	theme	charged	780:786	arg1	chitosan					813:820	chitosan	813:820	chitosan (water soluble)	813:836	Firstly, suspensions of water-in-oil microcapsules were formed in microfluidic junctions by self-assembly of two oppositely charged polyelectrolytes, namely chitosan (water soluble) and phosphatidic fatty acid (oil soluble).
28804800	2	53	theme	assembly	239:246	arg1	complex					342:348	complex	342:348	complex	342:348	The traditional method of layer-by-layer assembly of microcapsules allows building a tailored composite multi-layer membrane but is technically complex as it requires numerous steps.
28804800	2	53	theme	assembly	239:246	arg1	method					214:219	The traditional method	198:219	The traditional method of layer-by-layer assembly of microcapsules	198:263	The traditional method of layer-by-layer assembly of microcapsules allows building a tailored composite multi-layer membrane but is technically complex as it requires numerous steps.
28804800	8	54	theme	plasticity	1459:1468	arg1	terms					1438:1442	terms	1438:1442	terms of elasticity, plasticity and yield stress whose magnitudes can be controlled by the composition	1438:1539	Finally, we show that the design of microcapsules can be 'engineered' in an extensive way since they present a wealth of interfacial rheological properties in terms of elasticity, plasticity and yield stress whose magnitudes can be controlled by the composition.
28804800	1	55	dep	Tuning	80:85	arg1	properties					102:111	the mechanical properties	87:111	Tuning the mechanical properties of microcapsules through a cost-efficient route of fabrication	80:174	Tuning the mechanical properties of microcapsules through a cost-efficient route of fabrication is still a challenge.
28804800	5	56	theme	polyelectrolytes	788:803	arg1	self-assembly					748:760	self-assembly	748:760	self-assembly of two oppositely charged polyelectrolytes, namely chitosan (water soluble) and phosphatidic fatty acid (oil soluble)	748:878	Firstly, suspensions of water-in-oil microcapsules were formed in microfluidic junctions by self-assembly of two oppositely charged polyelectrolytes, namely chitosan (water soluble) and phosphatidic fatty acid (oil soluble).
28804800	2	57	theme	layer-by-layer	224:237	arg1	assembly					239:246	layer-by-layer assembly	224:246	layer-by-layer assembly of microcapsules	224:263	The traditional method of layer-by-layer assembly of microcapsules allows building a tailored composite multi-layer membrane but is technically complex as it requires numerous steps.
28804800	2	58	theme	multi-layer	302:312	arg1	membrane					314:321	a tailored composite multi-layer membrane	281:321	a tailored composite multi-layer membrane	281:321	The traditional method of layer-by-layer assembly of microcapsules allows building a tailored composite multi-layer membrane but is technically complex as it requires numerous steps.
28804800	1	59	theme	fabrication	164:174	arg1	route					155:159	a cost-efficient route	138:159	a cost-efficient route of fabrication	138:174	Tuning the mechanical properties of microcapsules through a cost-efficient route of fabrication is still a challenge.
28804800	3	60	theme	facile	546:551	arg1	step					553:556	one single facile step	535:556	one single facile step	535:556	The objective of this article is to characterize the interfacial rheological properties of self-assembling biopolymer microcapsules that were obtained in one single facile step.
28804800	5	61	theme	phosphatidic	842:853	arg1	oil					867:869	oil soluble	867:877	oil soluble	867:877	Firstly, suspensions of water-in-oil microcapsules were formed in microfluidic junctions by self-assembly of two oppositely charged polyelectrolytes, namely chitosan (water soluble) and phosphatidic fatty acid (oil soluble).
28804800	5	61	theme	phosphatidic	842:853	arg1	polyelectrolytes					788:803	two oppositely charged polyelectrolytes	765:803	two oppositely charged polyelectrolytes	765:803	Firstly, suspensions of water-in-oil microcapsules were formed in microfluidic junctions by self-assembly of two oppositely charged polyelectrolytes, namely chitosan (water soluble) and phosphatidic fatty acid (oil soluble).
28804800	5	61	theme	phosphatidic	842:853	arg1	acid					861:864	phosphatidic fatty acid	842:864	phosphatidic fatty acid (oil soluble)	842:878	Firstly, suspensions of water-in-oil microcapsules were formed in microfluidic junctions by self-assembly of two oppositely charged polyelectrolytes, namely chitosan (water soluble) and phosphatidic fatty acid (oil soluble).
28804800	6	62	theme	composite	894:902	arg1	membranes					904:912	composite membranes	894:912	composite membranes of tunable thickness (between 40 and 900 nm measured by AFM)	894:973	In this way, composite membranes of tunable thickness (between 40 and 900 nm measured by AFM) were formed at water/oil interfaces in a single step by changing the composition.
28804800	2	63	theme	composite	292:300	arg1	membrane					314:321	a tailored composite multi-layer membrane	281:321	a tailored composite multi-layer membrane	281:321	The traditional method of layer-by-layer assembly of microcapsules allows building a tailored composite multi-layer membrane but is technically complex as it requires numerous steps.
28804800	8	64	from	properties	1424:1433	arg1	terms					1438:1442	terms	1438:1442	terms of elasticity, plasticity and yield stress whose magnitudes can be controlled by the composition	1438:1539	Finally, we show that the design of microcapsules can be 'engineered' in an extensive way since they present a wealth of interfacial rheological properties in terms of elasticity, plasticity and yield stress whose magnitudes can be controlled by the composition.
28804800	8	65	theme	extensive	1355:1363	arg1	way					1365:1367	an extensive way	1352:1367	an extensive way	1352:1367	Finally, we show that the design of microcapsules can be 'engineered' in an extensive way since they present a wealth of interfacial rheological properties in terms of elasticity, plasticity and yield stress whose magnitudes can be controlled by the composition.
28804800	5	66	theme	fatty	855:859	arg1	oil					867:869	oil soluble	867:877	oil soluble	867:877	Firstly, suspensions of water-in-oil microcapsules were formed in microfluidic junctions by self-assembly of two oppositely charged polyelectrolytes, namely chitosan (water soluble) and phosphatidic fatty acid (oil soluble).
28804800	5	66	theme	fatty	855:859	arg1	polyelectrolytes					788:803	two oppositely charged polyelectrolytes	765:803	two oppositely charged polyelectrolytes	765:803	Firstly, suspensions of water-in-oil microcapsules were formed in microfluidic junctions by self-assembly of two oppositely charged polyelectrolytes, namely chitosan (water soluble) and phosphatidic fatty acid (oil soluble).
28804800	5	66	theme	fatty	855:859	arg1	acid					861:864	phosphatidic fatty acid	842:864	phosphatidic fatty acid (oil soluble)	842:878	Firstly, suspensions of water-in-oil microcapsules were formed in microfluidic junctions by self-assembly of two oppositely charged polyelectrolytes, namely chitosan (water soluble) and phosphatidic fatty acid (oil soluble).
28804800	2	67	theme	tailored	283:290	arg1	membrane					314:321	a tailored composite multi-layer membrane	281:321	a tailored composite multi-layer membrane	281:321	The traditional method of layer-by-layer assembly of microcapsules allows building a tailored composite multi-layer membrane but is technically complex as it requires numerous steps.
28804800	7	68	theme	hydrodynamic	1229:1240	arg1	method					1242:1247	the hydrodynamic method	1225:1247	the hydrodynamic method	1225:1247	Secondly, microcapsules were mechanically characterized by stretching them up to break-up in an extensional flow chamber which extends the relevance and convenience of the hydrodynamic method to weakly cohesive membranes.
28804800	7	69	theme	method	1242:1247	arg1	convenience					1210:1220	convenience	1210:1220	convenience	1210:1220	Secondly, microcapsules were mechanically characterized by stretching them up to break-up in an extensional flow chamber which extends the relevance and convenience of the hydrodynamic method to weakly cohesive membranes.
28804800	7	69	theme	method	1242:1247	arg1	relevance					1196:1204	relevance	1196:1204	relevance	1196:1204	Secondly, microcapsules were mechanically characterized by stretching them up to break-up in an extensional flow chamber which extends the relevance and convenience of the hydrodynamic method to weakly cohesive membranes.
27029842	2	0	theme	/TPU	405:408	arg1	materials					420:428	/TPU composite materials	405:428	/TPU composite materials	405:428	BiNFi-s (2 and 5 wt %)/TPU composite materials were prepared via compression molding, and glass fiber (2 and 5 wt %)/TPU composite materials and plain TPU were also prepared for comparison.
27029842	7	1	from	decrease	1383:1390	arg1	effect					1412:1417	the shape memory effect	1395:1417	the shape memory effect	1395:1417	The addition of 5 wt % BiNFi-s into TPU resulted in an improvement in the elastic modulus without any decrease in the shape memory effect.
27029842	4	2	theme	test	645:648	arg1	specimens					650:658	20-mm-long test specimens	634:658	20-mm-long test specimens with cross-sectional dimensions of 1 mm × 1 mm	634:705	20-mm-long test specimens with cross-sectional dimensions of 1 mm × 1 mm were cut from sheets of the composite materials, and three-point bending tests were carried out using a universal testing machine to investigate their mechanical properties and shape memory.
27029842	1	3	theme	polyether-based	216:230	arg1	TPU					260:262	TPU	260:262	TPU	260:262	We investigated the effects of the addition of chitosan fiber (biomass nanofiber made by Sugino (BiNFi-s)) to polyether-based thermoplastic polyurethane (TPU) on material properties.
27029842	1	3	theme	polyether-based	216:230	arg1	polyurethane					246:257	polyether-based thermoplastic polyurethane	216:257	polyether-based thermoplastic polyurethane (TPU)	216:263	We investigated the effects of the addition of chitosan fiber (biomass nanofiber made by Sugino (BiNFi-s)) to polyether-based thermoplastic polyurethane (TPU) on material properties.
27029842	7	4	theme	wt	1299:1300	arg1	BiNFi-s					1304:1310	5 wt % BiNFi-s	1297:1310	5 wt % BiNFi-s into TPU	1297:1319	The addition of 5 wt % BiNFi-s into TPU resulted in an improvement in the elastic modulus without any decrease in the shape memory effect.
27029842	8	5	dep	©	1420:1420	arg1	Inc.					1446:1449	Inc.	1446:1449	Inc.	1446:1449	© 2016 Wiley Periodicals, Inc.
27029842	4	6	theme	bending	772:778	arg1	tests					780:784	three-point bending tests	760:784	three-point bending tests	760:784	20-mm-long test specimens with cross-sectional dimensions of 1 mm × 1 mm were cut from sheets of the composite materials, and three-point bending tests were carried out using a universal testing machine to investigate their mechanical properties and shape memory.
27029842	1	7	theme	chitosan	153:160	arg1	fiber					162:166	chitosan fiber	153:166	chitosan fiber (biomass nanofiber made by Sugino (BiNFi-s))	153:211	We investigated the effects of the addition of chitosan fiber (biomass nanofiber made by Sugino (BiNFi-s)) to polyether-based thermoplastic polyurethane (TPU) on material properties.
27029842	1	8	theme	thermoplastic	232:244	arg1	TPU					260:262	TPU	260:262	TPU	260:262	We investigated the effects of the addition of chitosan fiber (biomass nanofiber made by Sugino (BiNFi-s)) to polyether-based thermoplastic polyurethane (TPU) on material properties.
27029842	1	8	theme	thermoplastic	232:244	arg1	polyurethane					246:257	polyether-based thermoplastic polyurethane	216:257	polyether-based thermoplastic polyurethane (TPU)	216:263	We investigated the effects of the addition of chitosan fiber (biomass nanofiber made by Sugino (BiNFi-s)) to polyether-based thermoplastic polyurethane (TPU) on material properties.
27029842	4	9	theme	shape	884:888	arg1	memory					890:895	shape memory	884:895	shape memory	884:895	20-mm-long test specimens with cross-sectional dimensions of 1 mm × 1 mm were cut from sheets of the composite materials, and three-point bending tests were carried out using a universal testing machine to investigate their mechanical properties and shape memory.
27029842	0	10	from	Effects	0:6	arg1	properties					42:51	the properties	38:51	the properties of polyurethane with thermo-responsive	38:90	Effects of chitosan fiber addition on the properties of polyurethane with thermo-responsive shape memory.
27029842	4	11	theme	mm	704:705	arg1	dimensions					681:690	cross-sectional dimensions	665:690	cross-sectional dimensions of 1 mm × 1 mm	665:705	20-mm-long test specimens with cross-sectional dimensions of 1 mm × 1 mm were cut from sheets of the composite materials, and three-point bending tests were carried out using a universal testing machine to investigate their mechanical properties and shape memory.
27029842	4	12	theme	mm	697:698	arg1	mm					704:705	1 mm × 1 mm	695:705	1 mm × 1 mm	695:705	20-mm-long test specimens with cross-sectional dimensions of 1 mm × 1 mm were cut from sheets of the composite materials, and three-point bending tests were carried out using a universal testing machine to investigate their mechanical properties and shape memory.
27029842	4	13	theme	1	695:695	arg1	mm					697:698	mm	697:698	mm	697:698	20-mm-long test specimens with cross-sectional dimensions of 1 mm × 1 mm were cut from sheets of the composite materials, and three-point bending tests were carried out using a universal testing machine to investigate their mechanical properties and shape memory.
27029842	7	14	theme	shape	1399:1403	arg1	effect					1412:1417	the shape memory effect	1395:1417	the shape memory effect	1395:1417	The addition of 5 wt % BiNFi-s into TPU resulted in an improvement in the elastic modulus without any decrease in the shape memory effect.
27029842	6	15	theme	wt	1126:1127	arg1	BiNFi-s					1131:1137	5 wt % BiNFi-s	1124:1137	5 wt % BiNFi-s (2.31 MPa)	1124:1148	The elastic modulus increased 40% by the addition of 5 wt % BiNFi-s (2.31 MPa) compared with plain TPU (1.65 MPa), and these composites exhibited shape recovery with clinically relevant changes in temperature.
27029842	6	15	theme	wt	1126:1127	arg1	MPa					1145:1147	2.31 MPa	1140:1147	2.31 MPa	1140:1147	The elastic modulus increased 40% by the addition of 5 wt % BiNFi-s (2.31 MPa) compared with plain TPU (1.65 MPa), and these composites exhibited shape recovery with clinically relevant changes in temperature.
27029842	0	16	with	polyurethane	56:67	arg1	thermo-responsive					74:90	thermo-responsive	74:90	thermo-responsive	74:90	Effects of chitosan fiber addition on the properties of polyurethane with thermo-responsive shape memory.
27029842	7	17	theme	elastic	1355:1361	arg1	modulus					1363:1369	the elastic modulus	1351:1369	the elastic modulus without any decrease in the shape memory effect	1351:1417	The addition of 5 wt % BiNFi-s into TPU resulted in an improvement in the elastic modulus without any decrease in the shape memory effect.
27029842	2	18	dep	BiNFi-s	289:295	arg1	%					309:309	2 and 5 wt %	298:309	2 and 5 wt %	298:309	BiNFi-s (2 and 5 wt %)/TPU composite materials were prepared via compression molding, and glass fiber (2 and 5 wt %)/TPU composite materials and plain TPU were also prepared for comparison.
27029842	2	19	theme	plain	434:438	arg1	TPU					440:442	plain TPU	434:442	plain TPU	434:442	BiNFi-s (2 and 5 wt %)/TPU composite materials were prepared via compression molding, and glass fiber (2 and 5 wt %)/TPU composite materials and plain TPU were also prepared for comparison.
27029842	2	20	dep	fiber	385:389	arg1	materials					420:428	/TPU composite materials	405:428	/TPU composite materials	405:428	BiNFi-s (2 and 5 wt %)/TPU composite materials were prepared via compression molding, and glass fiber (2 and 5 wt %)/TPU composite materials and plain TPU were also prepared for comparison.
27029842	7	21	theme	%	1302:1302	arg1	BiNFi-s					1304:1310	5 wt % BiNFi-s	1297:1310	5 wt % BiNFi-s into TPU	1297:1319	The addition of 5 wt % BiNFi-s into TPU resulted in an improvement in the elastic modulus without any decrease in the shape memory effect.
27029842	1	22	theme	fiber	162:166	arg1	addition					141:148	the addition	137:148	the addition of chitosan fiber (biomass nanofiber made by Sugino (BiNFi-s)) to polyether-based thermoplastic polyurethane (TPU)	137:263	We investigated the effects of the addition of chitosan fiber (biomass nanofiber made by Sugino (BiNFi-s)) to polyether-based thermoplastic polyurethane (TPU) on material properties.
27029842	9	23	dep	Res	1466:1468	arg1	1151-1156					1499:1507	1151-1156	1499:1507	1151-1156	1499:1507	J Biomed Mater Res Part B: Appl Biomater, 105B: 1151-1156, 2017.
27029842	9	23	dep	Res	1466:1468	arg1	105B					1493:1496	105B	1493:1496	105B	1493:1496	J Biomed Mater Res Part B: Appl Biomater, 105B: 1151-1156, 2017.
27029842	9	23	dep	Res	1466:1468	arg1	B					1475:1475	Part B	1470:1475	J Biomed Mater Res Part B: Appl Biomater, 105B: 1151-1156, 2017.	1451:1514	J Biomed Mater Res Part B: Appl Biomater, 105B: 1151-1156, 2017.
27029842	9	23	dep	Res	1466:1468	arg1	Biomater					1483:1490	Biomater	1483:1490	Biomater	1483:1490	J Biomed Mater Res Part B: Appl Biomater, 105B: 1151-1156, 2017.
27029842	6	24	from	changes	1257:1263	arg1	temperature					1268:1278	temperature	1268:1278	temperature	1268:1278	The elastic modulus increased 40% by the addition of 5 wt % BiNFi-s (2.31 MPa) compared with plain TPU (1.65 MPa), and these composites exhibited shape recovery with clinically relevant changes in temperature.
27029842	4	25	theme	three-point	760:770	arg1	bending					772:778	three-point bending	760:778	three-point bending tests	760:784	20-mm-long test specimens with cross-sectional dimensions of 1 mm × 1 mm were cut from sheets of the composite materials, and three-point bending tests were carried out using a universal testing machine to investigate their mechanical properties and shape memory.
27029842	1	26	from	effects	126:132	arg1	properties					277:286	material properties	268:286	material properties	268:286	We investigated the effects of the addition of chitosan fiber (biomass nanofiber made by Sugino (BiNFi-s)) to polyether-based thermoplastic polyurethane (TPU) on material properties.
27029842	5	27	theme	BiNFi-s	914:920	arg1	addition					902:909	The addition	898:909	The addition of BiNFi-s or glass fiber to TPU	898:942	The addition of BiNFi-s or glass fiber to TPU did not influence the glass transition temperature, although the crystal structure changed from semi-crystalline to amorphous.
27029842	3	28	theme	glass	483:487	arg1	temperature					500:510	The glass transition temperature	479:510	The glass transition temperature	479:510	The glass transition temperature was analyzed using differential scanning calorimetry, and the crystal structure was investigated using X-ray diffraction.
27029842	1	29	theme	biomass	169:175	arg1	nanofiber					177:185	biomass nanofiber	169:185	biomass nanofiber made by Sugino (BiNFi-s)	169:210	We investigated the effects of the addition of chitosan fiber (biomass nanofiber made by Sugino (BiNFi-s)) to polyether-based thermoplastic polyurethane (TPU) on material properties.
27029842	7	30	from	improvement	1336:1346	arg1	modulus					1363:1369	the elastic modulus	1351:1369	the elastic modulus without any decrease in the shape memory effect	1351:1417	The addition of 5 wt % BiNFi-s into TPU resulted in an improvement in the elastic modulus without any decrease in the shape memory effect.
27029842	6	31	theme	plain	1164:1168	arg1	TPU					1170:1172	plain TPU	1164:1172	plain TPU (1.65 MPa)	1164:1183	The elastic modulus increased 40% by the addition of 5 wt % BiNFi-s (2.31 MPa) compared with plain TPU (1.65 MPa), and these composites exhibited shape recovery with clinically relevant changes in temperature.
27029842	6	31	theme	plain	1164:1168	arg1	MPa					1180:1182	1.65 MPa	1175:1182	1.65 MPa	1175:1182	The elastic modulus increased 40% by the addition of 5 wt % BiNFi-s (2.31 MPa) compared with plain TPU (1.65 MPa), and these composites exhibited shape recovery with clinically relevant changes in temperature.
27029842	4	32	theme	cross-sectional	665:679	arg1	dimensions					681:690	cross-sectional dimensions	665:690	cross-sectional dimensions of 1 mm × 1 mm	665:705	20-mm-long test specimens with cross-sectional dimensions of 1 mm × 1 mm were cut from sheets of the composite materials, and three-point bending tests were carried out using a universal testing machine to investigate their mechanical properties and shape memory.
27029842	2	33	theme	BiNFi-s	289:295	arg1	materials					326:334	BiNFi-s (2 and 5 wt %)/TPU composite materials	289:334	BiNFi-s (2 and 5 wt %)/TPU composite materials	289:334	BiNFi-s (2 and 5 wt %)/TPU composite materials were prepared via compression molding, and glass fiber (2 and 5 wt %)/TPU composite materials and plain TPU were also prepared for comparison.
27029842	6	34	dep	increased	1091:1099	arg1	compared					1150:1157	compared	1150:1157	compared with plain TPU (1.65 MPa)	1150:1183	The elastic modulus increased 40% by the addition of 5 wt % BiNFi-s (2.31 MPa) compared with plain TPU (1.65 MPa), and these composites exhibited shape recovery with clinically relevant changes in temperature.
27029842	0	35	theme	fiber	20:24	arg1	addition					26:33	chitosan fiber addition	11:33	chitosan fiber addition	11:33	Effects of chitosan fiber addition on the properties of polyurethane with thermo-responsive shape memory.
27029842	5	36	theme	fiber	931:935	arg1	addition					902:909	The addition	898:909	The addition of BiNFi-s or glass fiber to TPU	898:942	The addition of BiNFi-s or glass fiber to TPU did not influence the glass transition temperature, although the crystal structure changed from semi-crystalline to amorphous.
27029842	6	37	theme	BiNFi-s	1131:1137	arg1	addition					1112:1119	the addition	1108:1119	the addition of 5 wt % BiNFi-s (2.31 MPa)	1108:1148	The elastic modulus increased 40% by the addition of 5 wt % BiNFi-s (2.31 MPa) compared with plain TPU (1.65 MPa), and these composites exhibited shape recovery with clinically relevant changes in temperature.
27029842	4	38	theme	×	700:700	arg1	mm					704:705	1 mm × 1 mm	695:705	1 mm × 1 mm	695:705	20-mm-long test specimens with cross-sectional dimensions of 1 mm × 1 mm were cut from sheets of the composite materials, and three-point bending tests were carried out using a universal testing machine to investigate their mechanical properties and shape memory.
27029842	2	39	theme	glass	379:383	arg1	fiber					385:389	glass fiber	379:389	glass fiber (2 and 5 wt %)	379:404	BiNFi-s (2 and 5 wt %)/TPU composite materials were prepared via compression molding, and glass fiber (2 and 5 wt %)/TPU composite materials and plain TPU were also prepared for comparison.
27029842	2	39	theme	glass	379:383	arg1	%					403:403	2 and 5 wt %	392:403	2 and 5 wt %	392:403	BiNFi-s (2 and 5 wt %)/TPU composite materials were prepared via compression molding, and glass fiber (2 and 5 wt %)/TPU composite materials and plain TPU were also prepared for comparison.
27029842	4	40	with	specimens	650:658	arg1	dimensions					681:690	cross-sectional dimensions	665:690	cross-sectional dimensions of 1 mm × 1 mm	665:705	20-mm-long test specimens with cross-sectional dimensions of 1 mm × 1 mm were cut from sheets of the composite materials, and three-point bending tests were carried out using a universal testing machine to investigate their mechanical properties and shape memory.
27029842	6	41	theme	shape	1217:1221	arg1	recovery					1223:1230	shape recovery	1217:1230	shape recovery	1217:1230	The elastic modulus increased 40% by the addition of 5 wt % BiNFi-s (2.31 MPa) compared with plain TPU (1.65 MPa), and these composites exhibited shape recovery with clinically relevant changes in temperature.
27029842	3	42	theme	crystal	574:580	arg1	structure					582:590	the crystal structure	570:590	the crystal structure	570:590	The glass transition temperature was analyzed using differential scanning calorimetry, and the crystal structure was investigated using X-ray diffraction.
27029842	4	43	theme	20-mm-long	634:643	arg1	specimens					650:658	20-mm-long test specimens	634:658	20-mm-long test specimens with cross-sectional dimensions of 1 mm × 1 mm	634:705	20-mm-long test specimens with cross-sectional dimensions of 1 mm × 1 mm were cut from sheets of the composite materials, and three-point bending tests were carried out using a universal testing machine to investigate their mechanical properties and shape memory.
27029842	1	44	theme	material	268:275	arg1	properties					277:286	material properties	268:286	material properties	268:286	We investigated the effects of the addition of chitosan fiber (biomass nanofiber made by Sugino (BiNFi-s)) to polyether-based thermoplastic polyurethane (TPU) on material properties.
27029842	7	45	theme	memory	1405:1410	arg1	effect					1412:1417	the shape memory effect	1395:1417	the shape memory effect	1395:1417	The addition of 5 wt % BiNFi-s into TPU resulted in an improvement in the elastic modulus without any decrease in the shape memory effect.
27029842	4	46	theme	materials	745:753	arg1	sheets					721:726	sheets	721:726	sheets of the composite materials	721:753	20-mm-long test specimens with cross-sectional dimensions of 1 mm × 1 mm were cut from sheets of the composite materials, and three-point bending tests were carried out using a universal testing machine to investigate their mechanical properties and shape memory.
27029842	3	47	theme	transition	489:498	arg1	temperature					500:510	The glass transition temperature	479:510	The glass transition temperature	479:510	The glass transition temperature was analyzed using differential scanning calorimetry, and the crystal structure was investigated using X-ray diffraction.
27029842	2	48	theme	wt	400:401	arg1	fiber					385:389	glass fiber	379:389	glass fiber (2 and 5 wt %)	379:404	BiNFi-s (2 and 5 wt %)/TPU composite materials were prepared via compression molding, and glass fiber (2 and 5 wt %)/TPU composite materials and plain TPU were also prepared for comparison.
27029842	2	48	theme	wt	400:401	arg1	%					403:403	2 and 5 wt %	392:403	2 and 5 wt %	392:403	BiNFi-s (2 and 5 wt %)/TPU composite materials were prepared via compression molding, and glass fiber (2 and 5 wt %)/TPU composite materials and plain TPU were also prepared for comparison.
27029842	0	49	theme	addition	26:33	arg1	Effects					0:6	Effects	0:6	Effects of chitosan fiber addition on the properties of polyurethane with thermo-responsive	0:90	Effects of chitosan fiber addition on the properties of polyurethane with thermo-responsive shape memory.
27029842	4	50	theme	composite	735:743	arg1	materials					745:753	the composite materials	731:753	the composite materials	731:753	20-mm-long test specimens with cross-sectional dimensions of 1 mm × 1 mm were cut from sheets of the composite materials, and three-point bending tests were carried out using a universal testing machine to investigate their mechanical properties and shape memory.
27029842	2	51	theme	compression	354:364	arg1	molding					366:372	compression molding	354:372	compression molding	354:372	BiNFi-s (2 and 5 wt %)/TPU composite materials were prepared via compression molding, and glass fiber (2 and 5 wt %)/TPU composite materials and plain TPU were also prepared for comparison.
27029842	4	52	theme	testing	821:827	arg1	machine					829:835	a universal testing machine	809:835	a universal testing machine	809:835	20-mm-long test specimens with cross-sectional dimensions of 1 mm × 1 mm were cut from sheets of the composite materials, and three-point bending tests were carried out using a universal testing machine to investigate their mechanical properties and shape memory.
27029842	5	53	theme	crystal	1009:1015	arg1	structure					1017:1025	the crystal structure	1005:1025	the crystal structure	1005:1025	The addition of BiNFi-s or glass fiber to TPU did not influence the glass transition temperature, although the crystal structure changed from semi-crystalline to amorphous.
27029842	1	54	dep	fiber	162:166	arg1	nanofiber					177:185	biomass nanofiber	169:185	biomass nanofiber made by Sugino (BiNFi-s)	169:210	We investigated the effects of the addition of chitosan fiber (biomass nanofiber made by Sugino (BiNFi-s)) to polyether-based thermoplastic polyurethane (TPU) on material properties.
27029842	2	55	theme	wt	306:307	arg1	%					309:309	2 and 5 wt %	298:309	2 and 5 wt %	298:309	BiNFi-s (2 and 5 wt %)/TPU composite materials were prepared via compression molding, and glass fiber (2 and 5 wt %)/TPU composite materials and plain TPU were also prepared for comparison.
27029842	4	56	theme	mechanical	858:867	arg1	properties					869:878	their mechanical properties	852:878	their mechanical properties	852:878	20-mm-long test specimens with cross-sectional dimensions of 1 mm × 1 mm were cut from sheets of the composite materials, and three-point bending tests were carried out using a universal testing machine to investigate their mechanical properties and shape memory.
27029842	4	57	theme	universal	811:819	arg1	machine					829:835	a universal testing machine	809:835	a universal testing machine	809:835	20-mm-long test specimens with cross-sectional dimensions of 1 mm × 1 mm were cut from sheets of the composite materials, and three-point bending tests were carried out using a universal testing machine to investigate their mechanical properties and shape memory.
27029842	5	58	theme	glass	966:970	arg1	transition					972:981	the glass transition	962:981	the glass transition temperature	962:993	The addition of BiNFi-s or glass fiber to TPU did not influence the glass transition temperature, although the crystal structure changed from semi-crystalline to amorphous.
27029842	3	59	theme	scanning	544:551	arg1	calorimetry					553:563	differential scanning calorimetry	531:563	differential scanning calorimetry	531:563	The glass transition temperature was analyzed using differential scanning calorimetry, and the crystal structure was investigated using X-ray diffraction.
27029842	3	60	theme	X-ray	615:619	arg1	diffraction					621:631	X-ray diffraction	615:631	X-ray diffraction	615:631	The glass transition temperature was analyzed using differential scanning calorimetry, and the crystal structure was investigated using X-ray diffraction.
27029842	6	61	theme	%	1129:1129	arg1	BiNFi-s					1131:1137	5 wt % BiNFi-s	1124:1137	5 wt % BiNFi-s (2.31 MPa)	1124:1148	The elastic modulus increased 40% by the addition of 5 wt % BiNFi-s (2.31 MPa) compared with plain TPU (1.65 MPa), and these composites exhibited shape recovery with clinically relevant changes in temperature.
27029842	6	61	theme	%	1129:1129	arg1	MPa					1145:1147	2.31 MPa	1140:1147	2.31 MPa	1140:1147	The elastic modulus increased 40% by the addition of 5 wt % BiNFi-s (2.31 MPa) compared with plain TPU (1.65 MPa), and these composites exhibited shape recovery with clinically relevant changes in temperature.
27029842	7	62	theme	BiNFi-s	1304:1310	arg1	addition					1285:1292	The addition	1281:1292	The addition of 5 wt % BiNFi-s into TPU	1281:1319	The addition of 5 wt % BiNFi-s into TPU resulted in an improvement in the elastic modulus without any decrease in the shape memory effect.
27029842	5	63	theme	transition	972:981	arg1	temperature					983:993	the glass transition temperature	962:993	the glass transition temperature	962:993	The addition of BiNFi-s or glass fiber to TPU did not influence the glass transition temperature, although the crystal structure changed from semi-crystalline to amorphous.
27029842	5	64	theme	glass	925:929	arg1	fiber					931:935	glass fiber	925:935	glass fiber	925:935	The addition of BiNFi-s or glass fiber to TPU did not influence the glass transition temperature, although the crystal structure changed from semi-crystalline to amorphous.
27029842	1	65	theme	addition	141:148	arg1	effects					126:132	the effects	122:132	the effects of the addition of chitosan fiber (biomass nanofiber made by Sugino (BiNFi-s)) to polyether-based thermoplastic polyurethane (TPU) on material properties	122:286	We investigated the effects of the addition of chitosan fiber (biomass nanofiber made by Sugino (BiNFi-s)) to polyether-based thermoplastic polyurethane (TPU) on material properties.
27029842	0	66	theme	polyurethane	56:67	arg1	properties					42:51	the properties	38:51	the properties of polyurethane with thermo-responsive	38:90	Effects of chitosan fiber addition on the properties of polyurethane with thermo-responsive shape memory.
27029842	3	67	theme	differential	531:542	arg1	calorimetry					553:563	differential scanning calorimetry	531:563	differential scanning calorimetry	531:563	The glass transition temperature was analyzed using differential scanning calorimetry, and the crystal structure was investigated using X-ray diffraction.
27029842	2	68	theme	composite	316:324	arg1	materials					326:334	BiNFi-s (2 and 5 wt %)/TPU composite materials	289:334	BiNFi-s (2 and 5 wt %)/TPU composite materials	289:334	BiNFi-s (2 and 5 wt %)/TPU composite materials were prepared via compression molding, and glass fiber (2 and 5 wt %)/TPU composite materials and plain TPU were also prepared for comparison.
27029842	2	69	theme	composite	410:418	arg1	materials					420:428	/TPU composite materials	405:428	/TPU composite materials	405:428	BiNFi-s (2 and 5 wt %)/TPU composite materials were prepared via compression molding, and glass fiber (2 and 5 wt %)/TPU composite materials and plain TPU were also prepared for comparison.
27029842	6	70	theme	elastic	1075:1081	arg1	modulus					1083:1089	The elastic modulus	1071:1089	The elastic modulus	1071:1089	The elastic modulus increased 40% by the addition of 5 wt % BiNFi-s (2.31 MPa) compared with plain TPU (1.65 MPa), and these composites exhibited shape recovery with clinically relevant changes in temperature.
27029842	0	71	theme	chitosan	11:18	arg1	addition					26:33	chitosan fiber addition	11:33	chitosan fiber addition	11:33	Effects of chitosan fiber addition on the properties of polyurethane with thermo-responsive shape memory.
27029842	2	72	theme	/TPU	311:314	arg1	materials					326:334	BiNFi-s (2 and 5 wt %)/TPU composite materials	289:334	BiNFi-s (2 and 5 wt %)/TPU composite materials	289:334	BiNFi-s (2 and 5 wt %)/TPU composite materials were prepared via compression molding, and glass fiber (2 and 5 wt %)/TPU composite materials and plain TPU were also prepared for comparison.
27029842	9	73	theme	Part	1470:1473	arg1	B					1475:1475	Part B	1470:1475	J Biomed Mater Res Part B: Appl Biomater, 105B: 1151-1156, 2017.	1451:1514	J Biomed Mater Res Part B: Appl Biomater, 105B: 1151-1156, 2017.
27029842	6	74	theme	relevant	1248:1255	arg1	changes					1257:1263	clinically relevant changes	1237:1263	clinically relevant changes in temperature	1237:1278	The elastic modulus increased 40% by the addition of 5 wt % BiNFi-s (2.31 MPa) compared with plain TPU (1.65 MPa), and these composites exhibited shape recovery with clinically relevant changes in temperature.
28401395	0	0	theme	cross-linked	94:105	arg1	membranes					144:152	cross-linked chitosan-graphene oxide mixed-matrix membranes	94:152	cross-linked chitosan-graphene oxide mixed-matrix membranes	94:152	Simultaneous wastewater treatment and bioelectricity production in microbial fuel cells using cross-linked chitosan-graphene oxide mixed-matrix membranes.
28401395	0	1	from	treatment	24:32	arg1	cells					82:86	microbial fuel cells	67:86	microbial fuel cells	67:86	Simultaneous wastewater treatment and bioelectricity production in microbial fuel cells using cross-linked chitosan-graphene oxide mixed-matrix membranes.
28401395	5	2	theme	CS-GO-S	1318:1324	arg1	system					1334:1339	the CS-GO-S(24) MFC system	1314:1339	the CS-GO-S(24) MFC system	1314:1339	Consequently, bioelectricity performance analysis in MFC application showed that CS-GO-P(24) membrane produced 135% higher power density than the CS-GO-S(24) MFC system.
28401395	6	3	theme	clarifier	1388:1396	arg1	wastewater					1408:1417	primary clarifier municipal wastewater	1380:1417	primary clarifier municipal wastewater	1380:1417	Simultaneously, 89.52% COD removal of primary clarifier municipal wastewater was achieved in the MFC operated with the CS-GO-P(24) membrane.
28401395	4	4	from	incorporation	815:827	arg1	composites					871:880	the CS-GO mixed-matrix composites	848:880	the CS-GO mixed-matrix composites	848:880	Interrogation of the physicochemical, thermal, and mechanical properties of the cross-linked CS-GO PEMs demonstrated that ionic cross-linking based on the incorporation of PO43- groups in the CS-GO mixed-matrix composites, when compared with sulfuric acid cross-linking commonly used in proton exchange membrane fuel cell (PEMFC) studies, generated additional density of ionic cluster domains, rendered enhanced sorption properties, and augmented the thermal and mechanical stability of the composite structure.
28401395	5	5	theme	MFC	1225:1227	arg1	application					1229:1239	MFC application	1225:1239	MFC application	1225:1239	Consequently, bioelectricity performance analysis in MFC application showed that CS-GO-P(24) membrane produced 135% higher power density than the CS-GO-S(24) MFC system.
28401395	4	6	link	cross-linked	740:751	arg1	PEMs					759:762	the cross-linked CS-GO PEMs	736:762	the cross-linked CS-GO PEMs	736:762	Interrogation of the physicochemical, thermal, and mechanical properties of the cross-linked CS-GO PEMs demonstrated that ionic cross-linking based on the incorporation of PO43- groups in the CS-GO mixed-matrix composites, when compared with sulfuric acid cross-linking commonly used in proton exchange membrane fuel cell (PEMFC) studies, generated additional density of ionic cluster domains, rendered enhanced sorption properties, and augmented the thermal and mechanical stability of the composite structure.
28401395	0	7	theme	oxide	125:129	arg1	membranes					144:152	cross-linked chitosan-graphene oxide mixed-matrix membranes	94:152	cross-linked chitosan-graphene oxide mixed-matrix membranes	94:152	Simultaneous wastewater treatment and bioelectricity production in microbial fuel cells using cross-linked chitosan-graphene oxide mixed-matrix membranes.
28401395	4	8	theme	mechanical	711:720	arg1	properties					722:731	the physicochemical, thermal, and mechanical properties	677:731	the physicochemical, thermal, and mechanical properties of the cross-linked CS-GO PEMs	677:762	Interrogation of the physicochemical, thermal, and mechanical properties of the cross-linked CS-GO PEMs demonstrated that ionic cross-linking based on the incorporation of PO43- groups in the CS-GO mixed-matrix composites, when compared with sulfuric acid cross-linking commonly used in proton exchange membrane fuel cell (PEMFC) studies, generated additional density of ionic cluster domains, rendered enhanced sorption properties, and augmented the thermal and mechanical stability of the composite structure.
28401395	6	9	theme	wastewater	1408:1417	arg1	removal					1369:1375	89.52% COD removal	1358:1375	89.52% COD removal of primary clarifier municipal wastewater	1358:1417	Simultaneously, 89.52% COD removal of primary clarifier municipal wastewater was achieved in the MFC operated with the CS-GO-P(24) membrane.
28401395	4	10	theme	thermal	698:704	arg1	properties					722:731	the physicochemical, thermal, and mechanical properties	677:731	the physicochemical, thermal, and mechanical properties of the cross-linked CS-GO PEMs	677:762	Interrogation of the physicochemical, thermal, and mechanical properties of the cross-linked CS-GO PEMs demonstrated that ionic cross-linking based on the incorporation of PO43- groups in the CS-GO mixed-matrix composites, when compared with sulfuric acid cross-linking commonly used in proton exchange membrane fuel cell (PEMFC) studies, generated additional density of ionic cluster domains, rendered enhanced sorption properties, and augmented the thermal and mechanical stability of the composite structure.
28401395	1	11	theme	simultaneous	278:289	arg1	production					306:315	simultaneous bioelectricity production	278:315	simultaneous bioelectricity production	278:315	Microbial fuel cells (MFCs) are emerging technology for wastewater treatment by chemical oxygen demand (COD) reduction and simultaneous bioelectricity production.
28401395	0	12	theme	chitosan-graphene	107:123	arg1	membranes					144:152	cross-linked chitosan-graphene oxide mixed-matrix membranes	94:152	cross-linked chitosan-graphene oxide mixed-matrix membranes	94:152	Simultaneous wastewater treatment and bioelectricity production in microbial fuel cells using cross-linked chitosan-graphene oxide mixed-matrix membranes.
28401395	4	13	theme	cell	977:980	arg1	studies					990:996	proton exchange membrane fuel cell (PEMFC) studies	947:996	proton exchange membrane fuel cell (PEMFC) studies	947:996	Interrogation of the physicochemical, thermal, and mechanical properties of the cross-linked CS-GO PEMs demonstrated that ionic cross-linking based on the incorporation of PO43- groups in the CS-GO mixed-matrix composites, when compared with sulfuric acid cross-linking commonly used in proton exchange membrane fuel cell (PEMFC) studies, generated additional density of ionic cluster domains, rendered enhanced sorption properties, and augmented the thermal and mechanical stability of the composite structure.
28401395	4	14	theme	sorption	1072:1079	arg1	properties					1081:1090	enhanced sorption properties	1063:1090	enhanced sorption properties	1063:1090	Interrogation of the physicochemical, thermal, and mechanical properties of the cross-linked CS-GO PEMs demonstrated that ionic cross-linking based on the incorporation of PO43- groups in the CS-GO mixed-matrix composites, when compared with sulfuric acid cross-linking commonly used in proton exchange membrane fuel cell (PEMFC) studies, generated additional density of ionic cluster domains, rendered enhanced sorption properties, and augmented the thermal and mechanical stability of the composite structure.
28401395	1	15	theme	bioelectricity	291:304	arg1	production					306:315	simultaneous bioelectricity production	278:315	simultaneous bioelectricity production	278:315	Microbial fuel cells (MFCs) are emerging technology for wastewater treatment by chemical oxygen demand (COD) reduction and simultaneous bioelectricity production.
28401395	4	16	theme	sulfuric	902:909	arg1	cross-linking					916:928	sulfuric acid cross-linking	902:928	sulfuric acid cross-linking commonly used in proton exchange membrane fuel cell (PEMFC) studies	902:996	Interrogation of the physicochemical, thermal, and mechanical properties of the cross-linked CS-GO PEMs demonstrated that ionic cross-linking based on the incorporation of PO43- groups in the CS-GO mixed-matrix composites, when compared with sulfuric acid cross-linking commonly used in proton exchange membrane fuel cell (PEMFC) studies, generated additional density of ionic cluster domains, rendered enhanced sorption properties, and augmented the thermal and mechanical stability of the composite structure.
28401395	4	17	theme	ionic	782:786	arg1	cross-linking					788:800	ionic cross-linking	782:800	ionic cross-linking based on the incorporation of PO43- groups in the CS-GO mixed-matrix composites	782:880	Interrogation of the physicochemical, thermal, and mechanical properties of the cross-linked CS-GO PEMs demonstrated that ionic cross-linking based on the incorporation of PO43- groups in the CS-GO mixed-matrix composites, when compared with sulfuric acid cross-linking commonly used in proton exchange membrane fuel cell (PEMFC) studies, generated additional density of ionic cluster domains, rendered enhanced sorption properties, and augmented the thermal and mechanical stability of the composite structure.
28401395	3	18	dep	oxide	494:498	arg1	GO					501:502	GO	501:502	GO	501:502	In this work, green chitosan-based (CS) PEMs were fabricated with graphene oxide (GO) as filler material (CS-GO) and cross-linked with phosphoric acid (CS-GO-P(24)) or sulfuric acid (CS-GO-S(24)) to determine their effect on PEM properties.
28401395	4	19	theme	enhanced	1063:1070	arg1	properties					1081:1090	enhanced sorption properties	1063:1090	enhanced sorption properties	1063:1090	Interrogation of the physicochemical, thermal, and mechanical properties of the cross-linked CS-GO PEMs demonstrated that ionic cross-linking based on the incorporation of PO43- groups in the CS-GO mixed-matrix composites, when compared with sulfuric acid cross-linking commonly used in proton exchange membrane fuel cell (PEMFC) studies, generated additional density of ionic cluster domains, rendered enhanced sorption properties, and augmented the thermal and mechanical stability of the composite structure.
28401395	1	20	theme	emerging	187:194	arg1	cells					170:174	Microbial fuel cells	155:174	Microbial fuel cells (MFCs)	155:181	Microbial fuel cells (MFCs) are emerging technology for wastewater treatment by chemical oxygen demand (COD) reduction and simultaneous bioelectricity production.
28401395	1	20	theme	emerging	187:194	arg1	technology					196:205	emerging technology	187:205	emerging technology for wastewater treatment	187:230	Microbial fuel cells (MFCs) are emerging technology for wastewater treatment by chemical oxygen demand (COD) reduction and simultaneous bioelectricity production.
28401395	0	21	theme	mixed-matrix	131:142	arg1	membranes					144:152	cross-linked chitosan-graphene oxide mixed-matrix membranes	94:152	cross-linked chitosan-graphene oxide mixed-matrix membranes	94:152	Simultaneous wastewater treatment and bioelectricity production in microbial fuel cells using cross-linked chitosan-graphene oxide mixed-matrix membranes.
28401395	0	22	from	production	53:62	arg1	cells					82:86	microbial fuel cells	67:86	microbial fuel cells	67:86	Simultaneous wastewater treatment and bioelectricity production in microbial fuel cells using cross-linked chitosan-graphene oxide mixed-matrix membranes.
28401395	4	23	theme	composite	1151:1159	arg1	structure					1161:1169	the composite structure	1147:1169	the composite structure	1147:1169	Interrogation of the physicochemical, thermal, and mechanical properties of the cross-linked CS-GO PEMs demonstrated that ionic cross-linking based on the incorporation of PO43- groups in the CS-GO mixed-matrix composites, when compared with sulfuric acid cross-linking commonly used in proton exchange membrane fuel cell (PEMFC) studies, generated additional density of ionic cluster domains, rendered enhanced sorption properties, and augmented the thermal and mechanical stability of the composite structure.
28401395	4	24	theme	fuel	972:975	arg1	studies					990:996	proton exchange membrane fuel cell (PEMFC) studies	947:996	proton exchange membrane fuel cell (PEMFC) studies	947:996	Interrogation of the physicochemical, thermal, and mechanical properties of the cross-linked CS-GO PEMs demonstrated that ionic cross-linking based on the incorporation of PO43- groups in the CS-GO mixed-matrix composites, when compared with sulfuric acid cross-linking commonly used in proton exchange membrane fuel cell (PEMFC) studies, generated additional density of ionic cluster domains, rendered enhanced sorption properties, and augmented the thermal and mechanical stability of the composite structure.
28401395	5	25	theme	higher	1288:1293	arg1	density					1301:1307	135% higher power density	1283:1307	135% higher power density	1283:1307	Consequently, bioelectricity performance analysis in MFC application showed that CS-GO-P(24) membrane produced 135% higher power density than the CS-GO-S(24) MFC system.
28401395	0	26	theme	wastewater	13:22	arg1	treatment					24:32	Simultaneous wastewater treatment	0:32	Simultaneous wastewater treatment	0:32	Simultaneous wastewater treatment and bioelectricity production in microbial fuel cells using cross-linked chitosan-graphene oxide mixed-matrix membranes.
28401395	3	27	theme	PEM	644:646	arg1	properties					648:657	PEM properties	644:657	PEM properties	644:657	In this work, green chitosan-based (CS) PEMs were fabricated with graphene oxide (GO) as filler material (CS-GO) and cross-linked with phosphoric acid (CS-GO-P(24)) or sulfuric acid (CS-GO-S(24)) to determine their effect on PEM properties.
28401395	2	28	theme	exchange	353:360	arg1	PEM					372:374	PEM	372:374	PEM	372:374	Fabrication of an effective proton exchange membrane (PEM) is a vital component for MFC performance.
28401395	2	28	theme	exchange	353:360	arg1	membrane					362:369	an effective proton exchange membrane	333:369	an effective proton exchange membrane (PEM)	333:375	Fabrication of an effective proton exchange membrane (PEM) is a vital component for MFC performance.
28401395	5	29	theme	power	1295:1299	arg1	density					1301:1307	135% higher power density	1283:1307	135% higher power density	1283:1307	Consequently, bioelectricity performance analysis in MFC application showed that CS-GO-P(24) membrane produced 135% higher power density than the CS-GO-S(24) MFC system.
28401395	2	30	theme	MFC	402:404	arg1	performance					406:416	MFC performance	402:416	MFC performance	402:416	Fabrication of an effective proton exchange membrane (PEM) is a vital component for MFC performance.
28401395	0	31	theme	Simultaneous	0:11	arg1	treatment					24:32	Simultaneous wastewater treatment	0:32	Simultaneous wastewater treatment	0:32	Simultaneous wastewater treatment and bioelectricity production in microbial fuel cells using cross-linked chitosan-graphene oxide mixed-matrix membranes.
28401395	4	32	theme	membrane	963:970	arg1	studies					990:996	proton exchange membrane fuel cell (PEMFC) studies	947:996	proton exchange membrane fuel cell (PEMFC) studies	947:996	Interrogation of the physicochemical, thermal, and mechanical properties of the cross-linked CS-GO PEMs demonstrated that ionic cross-linking based on the incorporation of PO43- groups in the CS-GO mixed-matrix composites, when compared with sulfuric acid cross-linking commonly used in proton exchange membrane fuel cell (PEMFC) studies, generated additional density of ionic cluster domains, rendered enhanced sorption properties, and augmented the thermal and mechanical stability of the composite structure.
28401395	2	33	theme	proton	346:351	arg1	PEM					372:374	PEM	372:374	PEM	372:374	Fabrication of an effective proton exchange membrane (PEM) is a vital component for MFC performance.
28401395	2	33	theme	proton	346:351	arg1	membrane					362:369	an effective proton exchange membrane	333:369	an effective proton exchange membrane (PEM)	333:375	Fabrication of an effective proton exchange membrane (PEM) is a vital component for MFC performance.
28401395	4	34	theme	domains	1045:1051	arg1	density					1020:1026	additional density	1009:1026	additional density of ionic cluster domains	1009:1051	Interrogation of the physicochemical, thermal, and mechanical properties of the cross-linked CS-GO PEMs demonstrated that ionic cross-linking based on the incorporation of PO43- groups in the CS-GO mixed-matrix composites, when compared with sulfuric acid cross-linking commonly used in proton exchange membrane fuel cell (PEMFC) studies, generated additional density of ionic cluster domains, rendered enhanced sorption properties, and augmented the thermal and mechanical stability of the composite structure.
28401395	1	35	theme	wastewater	211:220	arg1	treatment					222:230	wastewater treatment	211:230	wastewater treatment	211:230	Microbial fuel cells (MFCs) are emerging technology for wastewater treatment by chemical oxygen demand (COD) reduction and simultaneous bioelectricity production.
28401395	4	36	theme	PEMFC	983:987	arg1	studies					990:996	proton exchange membrane fuel cell (PEMFC) studies	947:996	proton exchange membrane fuel cell (PEMFC) studies	947:996	Interrogation of the physicochemical, thermal, and mechanical properties of the cross-linked CS-GO PEMs demonstrated that ionic cross-linking based on the incorporation of PO43- groups in the CS-GO mixed-matrix composites, when compared with sulfuric acid cross-linking commonly used in proton exchange membrane fuel cell (PEMFC) studies, generated additional density of ionic cluster domains, rendered enhanced sorption properties, and augmented the thermal and mechanical stability of the composite structure.
28401395	4	37	theme	mixed-matrix	858:869	arg1	composites					871:880	the CS-GO mixed-matrix composites	848:880	the CS-GO mixed-matrix composites	848:880	Interrogation of the physicochemical, thermal, and mechanical properties of the cross-linked CS-GO PEMs demonstrated that ionic cross-linking based on the incorporation of PO43- groups in the CS-GO mixed-matrix composites, when compared with sulfuric acid cross-linking commonly used in proton exchange membrane fuel cell (PEMFC) studies, generated additional density of ionic cluster domains, rendered enhanced sorption properties, and augmented the thermal and mechanical stability of the composite structure.
28401395	4	38	theme	structure	1161:1169	arg1	stability					1134:1142	the thermal and mechanical stability	1107:1142	the thermal and mechanical stability of the composite structure	1107:1169	Interrogation of the physicochemical, thermal, and mechanical properties of the cross-linked CS-GO PEMs demonstrated that ionic cross-linking based on the incorporation of PO43- groups in the CS-GO mixed-matrix composites, when compared with sulfuric acid cross-linking commonly used in proton exchange membrane fuel cell (PEMFC) studies, generated additional density of ionic cluster domains, rendered enhanced sorption properties, and augmented the thermal and mechanical stability of the composite structure.
28401395	2	39	theme	effective	336:344	arg1	PEM					372:374	PEM	372:374	PEM	372:374	Fabrication of an effective proton exchange membrane (PEM) is a vital component for MFC performance.
28401395	2	39	theme	effective	336:344	arg1	membrane					362:369	an effective proton exchange membrane	333:369	an effective proton exchange membrane (PEM)	333:375	Fabrication of an effective proton exchange membrane (PEM) is a vital component for MFC performance.
28401395	4	40	theme	cluster	1037:1043	arg1	domains					1045:1051	ionic cluster domains	1031:1051	ionic cluster domains	1031:1051	Interrogation of the physicochemical, thermal, and mechanical properties of the cross-linked CS-GO PEMs demonstrated that ionic cross-linking based on the incorporation of PO43- groups in the CS-GO mixed-matrix composites, when compared with sulfuric acid cross-linking commonly used in proton exchange membrane fuel cell (PEMFC) studies, generated additional density of ionic cluster domains, rendered enhanced sorption properties, and augmented the thermal and mechanical stability of the composite structure.
28401395	3	41	theme	phosphoric	554:563	arg1	CS-GO-P					571:577	CS-GO-P(24)	571:581	CS-GO-P(24)	571:581	In this work, green chitosan-based (CS) PEMs were fabricated with graphene oxide (GO) as filler material (CS-GO) and cross-linked with phosphoric acid (CS-GO-P(24)) or sulfuric acid (CS-GO-S(24)) to determine their effect on PEM properties.
28401395	3	41	theme	phosphoric	554:563	arg1	acid					565:568	phosphoric acid	554:568	phosphoric acid (CS-GO-P(24))	554:582	In this work, green chitosan-based (CS) PEMs were fabricated with graphene oxide (GO) as filler material (CS-GO) and cross-linked with phosphoric acid (CS-GO-P(24)) or sulfuric acid (CS-GO-S(24)) to determine their effect on PEM properties.
28401395	4	42	theme	CS-GO	852:856	arg1	composites					871:880	the CS-GO mixed-matrix composites	848:880	the CS-GO mixed-matrix composites	848:880	Interrogation of the physicochemical, thermal, and mechanical properties of the cross-linked CS-GO PEMs demonstrated that ionic cross-linking based on the incorporation of PO43- groups in the CS-GO mixed-matrix composites, when compared with sulfuric acid cross-linking commonly used in proton exchange membrane fuel cell (PEMFC) studies, generated additional density of ionic cluster domains, rendered enhanced sorption properties, and augmented the thermal and mechanical stability of the composite structure.
28401395	4	43	theme	physicochemical	681:695	arg1	properties					722:731	the physicochemical, thermal, and mechanical properties	677:731	the physicochemical, thermal, and mechanical properties of the cross-linked CS-GO PEMs	677:762	Interrogation of the physicochemical, thermal, and mechanical properties of the cross-linked CS-GO PEMs demonstrated that ionic cross-linking based on the incorporation of PO43- groups in the CS-GO mixed-matrix composites, when compared with sulfuric acid cross-linking commonly used in proton exchange membrane fuel cell (PEMFC) studies, generated additional density of ionic cluster domains, rendered enhanced sorption properties, and augmented the thermal and mechanical stability of the composite structure.
28401395	4	44	theme	proton	947:952	arg1	studies					990:996	proton exchange membrane fuel cell (PEMFC) studies	947:996	proton exchange membrane fuel cell (PEMFC) studies	947:996	Interrogation of the physicochemical, thermal, and mechanical properties of the cross-linked CS-GO PEMs demonstrated that ionic cross-linking based on the incorporation of PO43- groups in the CS-GO mixed-matrix composites, when compared with sulfuric acid cross-linking commonly used in proton exchange membrane fuel cell (PEMFC) studies, generated additional density of ionic cluster domains, rendered enhanced sorption properties, and augmented the thermal and mechanical stability of the composite structure.
28401395	6	45	theme	primary	1380:1386	arg1	wastewater					1408:1417	primary clarifier municipal wastewater	1380:1417	primary clarifier municipal wastewater	1380:1417	Simultaneously, 89.52% COD removal of primary clarifier municipal wastewater was achieved in the MFC operated with the CS-GO-P(24) membrane.
28401395	4	46	theme	CS-GO	753:757	arg1	PEMs					759:762	the cross-linked CS-GO PEMs	736:762	the cross-linked CS-GO PEMs	736:762	Interrogation of the physicochemical, thermal, and mechanical properties of the cross-linked CS-GO PEMs demonstrated that ionic cross-linking based on the incorporation of PO43- groups in the CS-GO mixed-matrix composites, when compared with sulfuric acid cross-linking commonly used in proton exchange membrane fuel cell (PEMFC) studies, generated additional density of ionic cluster domains, rendered enhanced sorption properties, and augmented the thermal and mechanical stability of the composite structure.
28401395	3	47	theme	chitosan-based	439:452	arg1	PEMs					459:462	green chitosan-based (CS) PEMs	433:462	green chitosan-based (CS) PEMs	433:462	In this work, green chitosan-based (CS) PEMs were fabricated with graphene oxide (GO) as filler material (CS-GO) and cross-linked with phosphoric acid (CS-GO-P(24)) or sulfuric acid (CS-GO-S(24)) to determine their effect on PEM properties.
28401395	3	47	theme	chitosan-based	439:452	arg1	material					515:522	filler material	508:522	filler material (CS-GO)	508:530	In this work, green chitosan-based (CS) PEMs were fabricated with graphene oxide (GO) as filler material (CS-GO) and cross-linked with phosphoric acid (CS-GO-P(24)) or sulfuric acid (CS-GO-S(24)) to determine their effect on PEM properties.
28401395	3	47	theme	chitosan-based	439:452	arg1	CS					455:456	CS	455:456	CS	455:456	In this work, green chitosan-based (CS) PEMs were fabricated with graphene oxide (GO) as filler material (CS-GO) and cross-linked with phosphoric acid (CS-GO-P(24)) or sulfuric acid (CS-GO-S(24)) to determine their effect on PEM properties.
28401395	1	48	theme	chemical	235:242	arg1	reduction					264:272	chemical oxygen demand (COD) reduction	235:272	chemical oxygen demand (COD) reduction	235:272	Microbial fuel cells (MFCs) are emerging technology for wastewater treatment by chemical oxygen demand (COD) reduction and simultaneous bioelectricity production.
28401395	0	49	theme	bioelectricity	38:51	arg1	production					53:62	bioelectricity production	38:62	bioelectricity production	38:62	Simultaneous wastewater treatment and bioelectricity production in microbial fuel cells using cross-linked chitosan-graphene oxide mixed-matrix membranes.
28401395	4	50	theme	exchange	954:961	arg1	studies					990:996	proton exchange membrane fuel cell (PEMFC) studies	947:996	proton exchange membrane fuel cell (PEMFC) studies	947:996	Interrogation of the physicochemical, thermal, and mechanical properties of the cross-linked CS-GO PEMs demonstrated that ionic cross-linking based on the incorporation of PO43- groups in the CS-GO mixed-matrix composites, when compared with sulfuric acid cross-linking commonly used in proton exchange membrane fuel cell (PEMFC) studies, generated additional density of ionic cluster domains, rendered enhanced sorption properties, and augmented the thermal and mechanical stability of the composite structure.
28401395	5	51	from	analysis	1213:1220	arg1	application					1229:1239	MFC application	1225:1239	MFC application	1225:1239	Consequently, bioelectricity performance analysis in MFC application showed that CS-GO-P(24) membrane produced 135% higher power density than the CS-GO-S(24) MFC system.
28401395	3	52	theme	sulfuric	587:594	arg1	CS-GO-S					602:608	CS-GO-S(24)	602:612	CS-GO-S(24)	602:612	In this work, green chitosan-based (CS) PEMs were fabricated with graphene oxide (GO) as filler material (CS-GO) and cross-linked with phosphoric acid (CS-GO-P(24)) or sulfuric acid (CS-GO-S(24)) to determine their effect on PEM properties.
28401395	3	52	theme	sulfuric	587:594	arg1	acid					596:599	sulfuric acid	587:599	sulfuric acid (CS-GO-S(24))	587:613	In this work, green chitosan-based (CS) PEMs were fabricated with graphene oxide (GO) as filler material (CS-GO) and cross-linked with phosphoric acid (CS-GO-P(24)) or sulfuric acid (CS-GO-S(24)) to determine their effect on PEM properties.
28401395	4	53	theme	cross-linked	740:751	arg1	PEMs					759:762	the cross-linked CS-GO PEMs	736:762	the cross-linked CS-GO PEMs	736:762	Interrogation of the physicochemical, thermal, and mechanical properties of the cross-linked CS-GO PEMs demonstrated that ionic cross-linking based on the incorporation of PO43- groups in the CS-GO mixed-matrix composites, when compared with sulfuric acid cross-linking commonly used in proton exchange membrane fuel cell (PEMFC) studies, generated additional density of ionic cluster domains, rendered enhanced sorption properties, and augmented the thermal and mechanical stability of the composite structure.
28401395	0	54	link	cross-linked	94:105	arg1	membranes					144:152	cross-linked chitosan-graphene oxide mixed-matrix membranes	94:152	cross-linked chitosan-graphene oxide mixed-matrix membranes	94:152	Simultaneous wastewater treatment and bioelectricity production in microbial fuel cells using cross-linked chitosan-graphene oxide mixed-matrix membranes.
28401395	1	55	theme	oxygen	244:249	arg1	COD					259:261	COD	259:261	COD	259:261	Microbial fuel cells (MFCs) are emerging technology for wastewater treatment by chemical oxygen demand (COD) reduction and simultaneous bioelectricity production.
28401395	1	55	theme	oxygen	244:249	arg1	demand					251:256	oxygen demand	244:256	chemical oxygen demand (COD) reduction	235:272	Microbial fuel cells (MFCs) are emerging technology for wastewater treatment by chemical oxygen demand (COD) reduction and simultaneous bioelectricity production.
28401395	0	56	theme	microbial	67:75	arg1	cells					82:86	microbial fuel cells	67:86	microbial fuel cells	67:86	Simultaneous wastewater treatment and bioelectricity production in microbial fuel cells using cross-linked chitosan-graphene oxide mixed-matrix membranes.
28401395	4	57	theme	groups	838:843	arg1	incorporation					815:827	the incorporation	811:827	the incorporation of PO43- groups in the CS-GO mixed-matrix composites	811:880	Interrogation of the physicochemical, thermal, and mechanical properties of the cross-linked CS-GO PEMs demonstrated that ionic cross-linking based on the incorporation of PO43- groups in the CS-GO mixed-matrix composites, when compared with sulfuric acid cross-linking commonly used in proton exchange membrane fuel cell (PEMFC) studies, generated additional density of ionic cluster domains, rendered enhanced sorption properties, and augmented the thermal and mechanical stability of the composite structure.
28401395	4	58	theme	acid	911:914	arg1	cross-linking					916:928	sulfuric acid cross-linking	902:928	sulfuric acid cross-linking commonly used in proton exchange membrane fuel cell (PEMFC) studies	902:996	Interrogation of the physicochemical, thermal, and mechanical properties of the cross-linked CS-GO PEMs demonstrated that ionic cross-linking based on the incorporation of PO43- groups in the CS-GO mixed-matrix composites, when compared with sulfuric acid cross-linking commonly used in proton exchange membrane fuel cell (PEMFC) studies, generated additional density of ionic cluster domains, rendered enhanced sorption properties, and augmented the thermal and mechanical stability of the composite structure.
28401395	6	59	theme	COD	1365:1367	arg1	removal					1369:1375	89.52% COD removal	1358:1375	89.52% COD removal of primary clarifier municipal wastewater	1358:1417	Simultaneously, 89.52% COD removal of primary clarifier municipal wastewater was achieved in the MFC operated with the CS-GO-P(24) membrane.
28401395	3	60	from	effect	634:639	arg1	properties					648:657	PEM properties	644:657	PEM properties	644:657	In this work, green chitosan-based (CS) PEMs were fabricated with graphene oxide (GO) as filler material (CS-GO) and cross-linked with phosphoric acid (CS-GO-P(24)) or sulfuric acid (CS-GO-S(24)) to determine their effect on PEM properties.
28401395	4	61	theme	PO43-	832:836	arg1	groups					838:843	PO43- groups	832:843	PO43- groups	832:843	Interrogation of the physicochemical, thermal, and mechanical properties of the cross-linked CS-GO PEMs demonstrated that ionic cross-linking based on the incorporation of PO43- groups in the CS-GO mixed-matrix composites, when compared with sulfuric acid cross-linking commonly used in proton exchange membrane fuel cell (PEMFC) studies, generated additional density of ionic cluster domains, rendered enhanced sorption properties, and augmented the thermal and mechanical stability of the composite structure.
28401395	4	62	theme	PEMs	759:762	arg1	properties					722:731	the physicochemical, thermal, and mechanical properties	677:731	the physicochemical, thermal, and mechanical properties of the cross-linked CS-GO PEMs	677:762	Interrogation of the physicochemical, thermal, and mechanical properties of the cross-linked CS-GO PEMs demonstrated that ionic cross-linking based on the incorporation of PO43- groups in the CS-GO mixed-matrix composites, when compared with sulfuric acid cross-linking commonly used in proton exchange membrane fuel cell (PEMFC) studies, generated additional density of ionic cluster domains, rendered enhanced sorption properties, and augmented the thermal and mechanical stability of the composite structure.
28401395	1	63	theme	Microbial	155:163	arg1	MFCs					177:180	MFCs	177:180	MFCs	177:180	Microbial fuel cells (MFCs) are emerging technology for wastewater treatment by chemical oxygen demand (COD) reduction and simultaneous bioelectricity production.
28401395	1	63	theme	Microbial	155:163	arg1	cells					170:174	Microbial fuel cells	155:174	Microbial fuel cells (MFCs)	155:181	Microbial fuel cells (MFCs) are emerging technology for wastewater treatment by chemical oxygen demand (COD) reduction and simultaneous bioelectricity production.
28401395	1	63	theme	Microbial	155:163	arg1	technology					196:205	emerging technology	187:205	emerging technology for wastewater treatment	187:230	Microbial fuel cells (MFCs) are emerging technology for wastewater treatment by chemical oxygen demand (COD) reduction and simultaneous bioelectricity production.
28401395	3	64	theme	filler	508:513	arg1	CS-GO					525:529	CS-GO	525:529	CS-GO	525:529	In this work, green chitosan-based (CS) PEMs were fabricated with graphene oxide (GO) as filler material (CS-GO) and cross-linked with phosphoric acid (CS-GO-P(24)) or sulfuric acid (CS-GO-S(24)) to determine their effect on PEM properties.
28401395	3	64	theme	filler	508:513	arg1	PEMs					459:462	green chitosan-based (CS) PEMs	433:462	green chitosan-based (CS) PEMs	433:462	In this work, green chitosan-based (CS) PEMs were fabricated with graphene oxide (GO) as filler material (CS-GO) and cross-linked with phosphoric acid (CS-GO-P(24)) or sulfuric acid (CS-GO-S(24)) to determine their effect on PEM properties.
28401395	3	64	theme	filler	508:513	arg1	material					515:522	filler material	508:522	filler material (CS-GO)	508:530	In this work, green chitosan-based (CS) PEMs were fabricated with graphene oxide (GO) as filler material (CS-GO) and cross-linked with phosphoric acid (CS-GO-P(24)) or sulfuric acid (CS-GO-S(24)) to determine their effect on PEM properties.
28401395	4	65	theme	properties	722:731	arg1	Interrogation					660:672	Interrogation	660:672	Interrogation of the physicochemical, thermal, and mechanical properties of the cross-linked CS-GO PEMs	660:762	Interrogation of the physicochemical, thermal, and mechanical properties of the cross-linked CS-GO PEMs demonstrated that ionic cross-linking based on the incorporation of PO43- groups in the CS-GO mixed-matrix composites, when compared with sulfuric acid cross-linking commonly used in proton exchange membrane fuel cell (PEMFC) studies, generated additional density of ionic cluster domains, rendered enhanced sorption properties, and augmented the thermal and mechanical stability of the composite structure.
28401395	5	66	theme	MFC	1330:1332	arg1	system					1334:1339	the CS-GO-S(24) MFC system	1314:1339	the CS-GO-S(24) MFC system	1314:1339	Consequently, bioelectricity performance analysis in MFC application showed that CS-GO-P(24) membrane produced 135% higher power density than the CS-GO-S(24) MFC system.
28401395	1	67	theme	fuel	165:168	arg1	MFCs					177:180	MFCs	177:180	MFCs	177:180	Microbial fuel cells (MFCs) are emerging technology for wastewater treatment by chemical oxygen demand (COD) reduction and simultaneous bioelectricity production.
28401395	1	67	theme	fuel	165:168	arg1	cells					170:174	Microbial fuel cells	155:174	Microbial fuel cells (MFCs)	155:181	Microbial fuel cells (MFCs) are emerging technology for wastewater treatment by chemical oxygen demand (COD) reduction and simultaneous bioelectricity production.
28401395	1	67	theme	fuel	165:168	arg1	technology					196:205	emerging technology	187:205	emerging technology for wastewater treatment	187:230	Microbial fuel cells (MFCs) are emerging technology for wastewater treatment by chemical oxygen demand (COD) reduction and simultaneous bioelectricity production.
28401395	4	68	theme	additional	1009:1018	arg1	density					1020:1026	additional density	1009:1026	additional density of ionic cluster domains	1009:1051	Interrogation of the physicochemical, thermal, and mechanical properties of the cross-linked CS-GO PEMs demonstrated that ionic cross-linking based on the incorporation of PO43- groups in the CS-GO mixed-matrix composites, when compared with sulfuric acid cross-linking commonly used in proton exchange membrane fuel cell (PEMFC) studies, generated additional density of ionic cluster domains, rendered enhanced sorption properties, and augmented the thermal and mechanical stability of the composite structure.
28401395	1	69	theme	demand	251:256	arg1	reduction					264:272	chemical oxygen demand (COD) reduction	235:272	chemical oxygen demand (COD) reduction	235:272	Microbial fuel cells (MFCs) are emerging technology for wastewater treatment by chemical oxygen demand (COD) reduction and simultaneous bioelectricity production.
28401395	4	70	theme	mechanical	1123:1132	arg1	stability					1134:1142	the thermal and mechanical stability	1107:1142	the thermal and mechanical stability of the composite structure	1107:1169	Interrogation of the physicochemical, thermal, and mechanical properties of the cross-linked CS-GO PEMs demonstrated that ionic cross-linking based on the incorporation of PO43- groups in the CS-GO mixed-matrix composites, when compared with sulfuric acid cross-linking commonly used in proton exchange membrane fuel cell (PEMFC) studies, generated additional density of ionic cluster domains, rendered enhanced sorption properties, and augmented the thermal and mechanical stability of the composite structure.
28401395	2	71	theme	membrane	362:369	arg1	Fabrication					318:328	Fabrication	318:328	Fabrication of an effective proton exchange membrane (PEM)	318:375	Fabrication of an effective proton exchange membrane (PEM) is a vital component for MFC performance.
28401395	2	71	theme	membrane	362:369	arg1	component					388:396	a vital component	380:396	a vital component for MFC performance	380:416	Fabrication of an effective proton exchange membrane (PEM) is a vital component for MFC performance.
28401395	4	72	theme	ionic	1031:1035	arg1	domains					1045:1051	ionic cluster domains	1031:1051	ionic cluster domains	1031:1051	Interrogation of the physicochemical, thermal, and mechanical properties of the cross-linked CS-GO PEMs demonstrated that ionic cross-linking based on the incorporation of PO43- groups in the CS-GO mixed-matrix composites, when compared with sulfuric acid cross-linking commonly used in proton exchange membrane fuel cell (PEMFC) studies, generated additional density of ionic cluster domains, rendered enhanced sorption properties, and augmented the thermal and mechanical stability of the composite structure.
28401395	6	73	theme	CS-GO-P	1461:1467	arg1	membrane					1473:1480	the CS-GO-P(24) membrane	1457:1480	the CS-GO-P(24) membrane	1457:1480	Simultaneously, 89.52% COD removal of primary clarifier municipal wastewater was achieved in the MFC operated with the CS-GO-P(24) membrane.
28401395	6	74	theme	%	1363:1363	arg1	removal					1369:1375	89.52% COD removal	1358:1375	89.52% COD removal of primary clarifier municipal wastewater	1358:1417	Simultaneously, 89.52% COD removal of primary clarifier municipal wastewater was achieved in the MFC operated with the CS-GO-P(24) membrane.
28401395	6	75	theme	municipal	1398:1406	arg1	wastewater					1408:1417	primary clarifier municipal wastewater	1380:1417	primary clarifier municipal wastewater	1380:1417	Simultaneously, 89.52% COD removal of primary clarifier municipal wastewater was achieved in the MFC operated with the CS-GO-P(24) membrane.
28401395	3	76	theme	graphene	485:492	arg1	oxide					494:498	graphene oxide	485:498	graphene oxide (GO)	485:503	In this work, green chitosan-based (CS) PEMs were fabricated with graphene oxide (GO) as filler material (CS-GO) and cross-linked with phosphoric acid (CS-GO-P(24)) or sulfuric acid (CS-GO-S(24)) to determine their effect on PEM properties.
28401395	5	77	theme	bioelectricity	1186:1199	arg1	analysis					1213:1220	bioelectricity performance analysis	1186:1220	bioelectricity performance analysis in MFC application	1186:1239	Consequently, bioelectricity performance analysis in MFC application showed that CS-GO-P(24) membrane produced 135% higher power density than the CS-GO-S(24) MFC system.
28401395	0	78	theme	fuel	77:80	arg1	cells					82:86	microbial fuel cells	67:86	microbial fuel cells	67:86	Simultaneous wastewater treatment and bioelectricity production in microbial fuel cells using cross-linked chitosan-graphene oxide mixed-matrix membranes.
28401395	6	79	theme	89.52	1358:1362	arg1	%					1363:1363	%	1363:1363	%	1363:1363	Simultaneously, 89.52% COD removal of primary clarifier municipal wastewater was achieved in the MFC operated with the CS-GO-P(24) membrane.
28401395	4	80	theme	thermal	1111:1117	arg1	stability					1134:1142	the thermal and mechanical stability	1107:1142	the thermal and mechanical stability of the composite structure	1107:1169	Interrogation of the physicochemical, thermal, and mechanical properties of the cross-linked CS-GO PEMs demonstrated that ionic cross-linking based on the incorporation of PO43- groups in the CS-GO mixed-matrix composites, when compared with sulfuric acid cross-linking commonly used in proton exchange membrane fuel cell (PEMFC) studies, generated additional density of ionic cluster domains, rendered enhanced sorption properties, and augmented the thermal and mechanical stability of the composite structure.
28401395	2	81	theme	vital	382:386	arg1	Fabrication					318:328	Fabrication	318:328	Fabrication of an effective proton exchange membrane (PEM)	318:375	Fabrication of an effective proton exchange membrane (PEM) is a vital component for MFC performance.
28401395	2	81	theme	vital	382:386	arg1	component					388:396	a vital component	380:396	a vital component for MFC performance	380:416	Fabrication of an effective proton exchange membrane (PEM) is a vital component for MFC performance.
28401395	5	82	theme	CS-GO-P	1253:1259	arg1	membrane					1265:1272	CS-GO-P(24) membrane	1253:1272	CS-GO-P(24) membrane	1253:1272	Consequently, bioelectricity performance analysis in MFC application showed that CS-GO-P(24) membrane produced 135% higher power density than the CS-GO-S(24) MFC system.
28401395	5	83	theme	performance	1201:1211	arg1	analysis					1213:1220	bioelectricity performance analysis	1186:1220	bioelectricity performance analysis in MFC application	1186:1239	Consequently, bioelectricity performance analysis in MFC application showed that CS-GO-P(24) membrane produced 135% higher power density than the CS-GO-S(24) MFC system.
24872221	6	0	theme	rheological	879:889	arg1	properties					891:900	good rheological properties	874:900	good rheological properties	874:900	The EPS was extracted and it displayed good rheological properties, moisture-retention ability, and antioxidant activity.
24872221	8	1	theme	high	1094:1097	arg1	activity					1111:1118	high antioxidant activity	1094:1118	high antioxidant activity	1094:1118	SM-A87 EPS also showed high antioxidant activity.
24872221	0	2	theme	low-cost	80:87	arg1	fermentation					89:100	low-cost fermentation	80:100	low-cost fermentation	80:100	A novel exopolysaccharide from deep-sea bacterium Zunongwangia profunda SM-A87: low-cost fermentation, moisture retention, and antioxidant activities.
24872221	6	3	theme	good	874:877	arg1	properties					891:900	good rheological properties	874:900	good rheological properties	874:900	The EPS was extracted and it displayed good rheological properties, moisture-retention ability, and antioxidant activity.
24872221	2	4	from	SM-A87	343:348	arg1	EPS					293:295	a novel EPS	285:295	a novel EPS from deep-sea bacterium Zunongwangia profunda SM-A87	285:348	We have purified a novel EPS from deep-sea bacterium Zunongwangia profunda SM-A87, identified its glycosyl composition and linkage, and optimized its production to 8.9 g/l in previous studies.
24872221	0	5	theme	moisture	103:110	arg1	retention					112:120	moisture retention	103:120	moisture retention	103:120	A novel exopolysaccharide from deep-sea bacterium Zunongwangia profunda SM-A87: low-cost fermentation, moisture retention, and antioxidant activities.
24872221	9	6	theme	promising	1163:1171	arg1	potentials					1173:1182	promising potentials	1163:1182	promising potentials	1163:1182	These results suggest that SM-A87 EPS has promising potentials in biotechnology.
24872221	2	7	theme	deep-sea	302:309	arg1	SM-A87					343:348	deep-sea bacterium Zunongwangia profunda SM-A87	302:348	deep-sea bacterium Zunongwangia profunda SM-A87	302:348	We have purified a novel EPS from deep-sea bacterium Zunongwangia profunda SM-A87, identified its glycosyl composition and linkage, and optimized its production to 8.9 g/l in previous studies.
24872221	3	8	theme	meal	567:570	arg1	NaCl					583:586	60.9 % whey, 10 g/l soybean meal, and 2.9 % NaCl	539:586	60.9 % whey, 10 g/l soybean meal, and 2.9 % NaCl	539:586	To reduce the fermentation cost, an economical fermentation medium containing 60.9 % whey, 10 g/l soybean meal, and 2.9 % NaCl was developed.
24872221	3	9	theme	g/l	555:557	arg1	meal					567:570	10 g/l soybean meal	552:570	10 g/l soybean meal	552:570	To reduce the fermentation cost, an economical fermentation medium containing 60.9 % whey, 10 g/l soybean meal, and 2.9 % NaCl was developed.
24872221	3	10	theme	economical	497:506	arg1	medium					521:526	an economical fermentation medium	494:526	an economical fermentation medium containing 60.9 % whey, 10 g/l soybean meal, and 2.9 % NaCl	494:586	To reduce the fermentation cost, an economical fermentation medium containing 60.9 % whey, 10 g/l soybean meal, and 2.9 % NaCl was developed.
24872221	5	11	theme	highest	779:785	arg1	yield					791:795	the highest EPS yield	775:795	the highest EPS yield ever reported for a marine bacterium	775:832	Fed-batch fermentation was conducted and led to an EPS yield of 17.2 ± 0.4 g/l, which represents the highest EPS yield ever reported for a marine bacterium.
24872221	3	12	theme	whey	546:549	arg1	%					544:544	60.9 % whey	539:549	60.9 % whey	539:549	To reduce the fermentation cost, an economical fermentation medium containing 60.9 % whey, 10 g/l soybean meal, and 2.9 % NaCl was developed.
24872221	5	13	theme	EPS	729:731	arg1	yield					733:737	an EPS yield	726:737	an EPS yield	726:737	Fed-batch fermentation was conducted and led to an EPS yield of 17.2 ± 0.4 g/l, which represents the highest EPS yield ever reported for a marine bacterium.
24872221	2	14	theme	novel	287:291	arg1	EPS					293:295	a novel EPS	285:295	a novel EPS from deep-sea bacterium Zunongwangia profunda SM-A87	285:348	We have purified a novel EPS from deep-sea bacterium Zunongwangia profunda SM-A87, identified its glycosyl composition and linkage, and optimized its production to 8.9 g/l in previous studies.
24872221	0	15	theme	novel	2:6	arg1	exopolysaccharide					8:24	A novel exopolysaccharide	0:24	A novel exopolysaccharide from deep-sea bacterium Zunongwangia profunda SM-A87: low-cost fermentation, moisture retention, and antioxidant activities.	0:149	A novel exopolysaccharide from deep-sea bacterium Zunongwangia profunda SM-A87: low-cost fermentation, moisture retention, and antioxidant activities.
24872221	5	16	theme	g/l	753:755	arg1	yield					733:737	an EPS yield	726:737	an EPS yield	726:737	Fed-batch fermentation was conducted and led to an EPS yield of 17.2 ± 0.4 g/l, which represents the highest EPS yield ever reported for a marine bacterium.
24872221	5	17	theme	EPS	787:789	arg1	yield					791:795	the highest EPS yield	775:795	the highest EPS yield ever reported for a marine bacterium	775:832	Fed-batch fermentation was conducted and led to an EPS yield of 17.2 ± 0.4 g/l, which represents the highest EPS yield ever reported for a marine bacterium.
24872221	2	18	theme	profunda	334:341	arg1	SM-A87					343:348	deep-sea bacterium Zunongwangia profunda SM-A87	302:348	deep-sea bacterium Zunongwangia profunda SM-A87	302:348	We have purified a novel EPS from deep-sea bacterium Zunongwangia profunda SM-A87, identified its glycosyl composition and linkage, and optimized its production to 8.9 g/l in previous studies.
24872221	0	19	theme	antioxidant	127:137	arg1	activities					139:148	antioxidant activities	127:148	antioxidant activities	127:148	A novel exopolysaccharide from deep-sea bacterium Zunongwangia profunda SM-A87: low-cost fermentation, moisture retention, and antioxidant activities.
24872221	2	20	theme	Zunongwangia	321:332	arg1	SM-A87					343:348	deep-sea bacterium Zunongwangia profunda SM-A87	302:348	deep-sea bacterium Zunongwangia profunda SM-A87	302:348	We have purified a novel EPS from deep-sea bacterium Zunongwangia profunda SM-A87, identified its glycosyl composition and linkage, and optimized its production to 8.9 g/l in previous studies.
24872221	3	21	theme	fermentation	475:486	arg1	cost					488:491	the fermentation cost	471:491	the fermentation cost	471:491	To reduce the fermentation cost, an economical fermentation medium containing 60.9 % whey, 10 g/l soybean meal, and 2.9 % NaCl was developed.
24872221	2	22	theme	previous	443:450	arg1	studies					452:458	previous studies	443:458	previous studies	443:458	We have purified a novel EPS from deep-sea bacterium Zunongwangia profunda SM-A87, identified its glycosyl composition and linkage, and optimized its production to 8.9 g/l in previous studies.
24872221	6	23	theme	antioxidant	935:945	arg1	activity					947:954	antioxidant activity	935:954	antioxidant activity	935:954	The EPS was extracted and it displayed good rheological properties, moisture-retention ability, and antioxidant activity.
24872221	2	24	theme	bacterium	311:319	arg1	SM-A87					343:348	deep-sea bacterium Zunongwangia profunda SM-A87	302:348	deep-sea bacterium Zunongwangia profunda SM-A87	302:348	We have purified a novel EPS from deep-sea bacterium Zunongwangia profunda SM-A87, identified its glycosyl composition and linkage, and optimized its production to 8.9 g/l in previous studies.
24872221	0	25	theme	bacterium	40:48	arg1	SM-A87					72:77	deep-sea bacterium Zunongwangia profunda SM-A87	31:77	deep-sea bacterium Zunongwangia profunda SM-A87	31:77	A novel exopolysaccharide from deep-sea bacterium Zunongwangia profunda SM-A87: low-cost fermentation, moisture retention, and antioxidant activities.
24872221	7	26	theme	bacterial	1038:1046	arg1	EPSs					1048:1051	other marine bacterial EPSs	1025:1051	other marine bacterial EPSs reported to date	1025:1068	Particularly, its moisture-retention ability is superior to that of other marine bacterial EPSs reported to date.
24872221	4	27	from	yield	611:615	arg1	medium					647:652	this medium	642:652	this medium	642:652	The EPS yield of batch fermentation in this medium reached 12.1 ± 0.3 g/l.
24872221	4	28	theme	fermentation	626:637	arg1	yield					611:615	The EPS yield	603:615	The EPS yield of batch fermentation in this medium	603:652	The EPS yield of batch fermentation in this medium reached 12.1 ± 0.3 g/l.
24872221	0	29	theme	deep-sea	31:38	arg1	SM-A87					72:77	deep-sea bacterium Zunongwangia profunda SM-A87	31:77	deep-sea bacterium Zunongwangia profunda SM-A87	31:77	A novel exopolysaccharide from deep-sea bacterium Zunongwangia profunda SM-A87: low-cost fermentation, moisture retention, and antioxidant activities.
24872221	8	30	theme	SM-A87	1071:1076	arg1	EPS					1078:1080	SM-A87 EPS	1071:1080	SM-A87 EPS	1071:1080	SM-A87 EPS also showed high antioxidant activity.
24872221	5	31	theme	marine	817:822	arg1	bacterium					824:832	a marine bacterium	815:832	a marine bacterium	815:832	Fed-batch fermentation was conducted and led to an EPS yield of 17.2 ± 0.4 g/l, which represents the highest EPS yield ever reported for a marine bacterium.
24872221	1	32	theme	important	227:235	arg1	applications					237:248	important applications	227:248	important applications	227:248	Many marine microorganisms can secrete exopolysaccharides (EPSs) which have important applications in biotechnology.
24872221	0	33	theme	profunda	63:70	arg1	SM-A87					72:77	deep-sea bacterium Zunongwangia profunda SM-A87	31:77	deep-sea bacterium Zunongwangia profunda SM-A87	31:77	A novel exopolysaccharide from deep-sea bacterium Zunongwangia profunda SM-A87: low-cost fermentation, moisture retention, and antioxidant activities.
24872221	7	34	theme	other	1025:1029	arg1	EPSs					1048:1051	other marine bacterial EPSs	1025:1051	other marine bacterial EPSs reported to date	1025:1068	Particularly, its moisture-retention ability is superior to that of other marine bacterial EPSs reported to date.
24872221	0	35	from	SM-A87	72:77	arg1	exopolysaccharide					8:24	A novel exopolysaccharide	0:24	A novel exopolysaccharide from deep-sea bacterium Zunongwangia profunda SM-A87: low-cost fermentation, moisture retention, and antioxidant activities.	0:149	A novel exopolysaccharide from deep-sea bacterium Zunongwangia profunda SM-A87: low-cost fermentation, moisture retention, and antioxidant activities.
24872221	2	36	theme	glycosyl	366:373	arg1	composition					375:385	its glycosyl composition	362:385	its glycosyl composition	362:385	We have purified a novel EPS from deep-sea bacterium Zunongwangia profunda SM-A87, identified its glycosyl composition and linkage, and optimized its production to 8.9 g/l in previous studies.
24872221	0	37	theme	Zunongwangia	50:61	arg1	SM-A87					72:77	deep-sea bacterium Zunongwangia profunda SM-A87	31:77	deep-sea bacterium Zunongwangia profunda SM-A87	31:77	A novel exopolysaccharide from deep-sea bacterium Zunongwangia profunda SM-A87: low-cost fermentation, moisture retention, and antioxidant activities.
24872221	6	38	theme	moisture-retention	903:920	arg1	ability					922:928	moisture-retention ability	903:928	moisture-retention ability	903:928	The EPS was extracted and it displayed good rheological properties, moisture-retention ability, and antioxidant activity.
24872221	7	39	theme	marine	1031:1036	arg1	EPSs					1048:1051	other marine bacterial EPSs	1025:1051	other marine bacterial EPSs reported to date	1025:1068	Particularly, its moisture-retention ability is superior to that of other marine bacterial EPSs reported to date.
24872221	3	40	theme	%	581:581	arg1	NaCl					583:586	60.9 % whey, 10 g/l soybean meal, and 2.9 % NaCl	539:586	60.9 % whey, 10 g/l soybean meal, and 2.9 % NaCl	539:586	To reduce the fermentation cost, an economical fermentation medium containing 60.9 % whey, 10 g/l soybean meal, and 2.9 % NaCl was developed.
24872221	2	41	from	production	418:427	arg1	studies					452:458	previous studies	443:458	previous studies	443:458	We have purified a novel EPS from deep-sea bacterium Zunongwangia profunda SM-A87, identified its glycosyl composition and linkage, and optimized its production to 8.9 g/l in previous studies.
24872221	3	42	theme	fermentation	508:519	arg1	medium					521:526	an economical fermentation medium	494:526	an economical fermentation medium containing 60.9 % whey, 10 g/l soybean meal, and 2.9 % NaCl	494:586	To reduce the fermentation cost, an economical fermentation medium containing 60.9 % whey, 10 g/l soybean meal, and 2.9 % NaCl was developed.
24872221	7	43	theme	moisture-retention	975:992	arg1	ability					994:1000	its moisture-retention ability	971:1000	its moisture-retention ability	971:1000	Particularly, its moisture-retention ability is superior to that of other marine bacterial EPSs reported to date.
24872221	8	44	theme	antioxidant	1099:1109	arg1	activity					1111:1118	high antioxidant activity	1094:1118	high antioxidant activity	1094:1118	SM-A87 EPS also showed high antioxidant activity.
24872221	1	45	contain	have	222:225	arg2	applications					237:248	important applications	227:248	important applications	227:248	Many marine microorganisms can secrete exopolysaccharides (EPSs) which have important applications in biotechnology.
24872221	1	45	contain	have	222:225	arg1	EPSs					210:213	EPSs	210:213	EPSs	210:213	Many marine microorganisms can secrete exopolysaccharides (EPSs) which have important applications in biotechnology.
24872221	1	45	contain	have	222:225	arg1	exopolysaccharides					190:207	exopolysaccharides	190:207	exopolysaccharides (EPSs) which have important applications in biotechnology	190:265	Many marine microorganisms can secrete exopolysaccharides (EPSs) which have important applications in biotechnology.
24872221	3	46	theme	soybean	559:565	arg1	meal					567:570	10 g/l soybean meal	552:570	10 g/l soybean meal	552:570	To reduce the fermentation cost, an economical fermentation medium containing 60.9 % whey, 10 g/l soybean meal, and 2.9 % NaCl was developed.
24872221	5	47	theme	Fed-batch	678:686	arg1	fermentation					688:699	Fed-batch fermentation	678:699	Fed-batch fermentation	678:699	Fed-batch fermentation was conducted and led to an EPS yield of 17.2 ± 0.4 g/l, which represents the highest EPS yield ever reported for a marine bacterium.
24872221	0	48	dep	exopolysaccharide	8:24	arg1	activities					139:148	antioxidant activities	127:148	antioxidant activities	127:148	A novel exopolysaccharide from deep-sea bacterium Zunongwangia profunda SM-A87: low-cost fermentation, moisture retention, and antioxidant activities.
24872221	0	48	dep	exopolysaccharide	8:24	arg1	fermentation					89:100	low-cost fermentation	80:100	low-cost fermentation	80:100	A novel exopolysaccharide from deep-sea bacterium Zunongwangia profunda SM-A87: low-cost fermentation, moisture retention, and antioxidant activities.
24872221	0	48	dep	exopolysaccharide	8:24	arg1	retention					112:120	moisture retention	103:120	moisture retention	103:120	A novel exopolysaccharide from deep-sea bacterium Zunongwangia profunda SM-A87: low-cost fermentation, moisture retention, and antioxidant activities.
24872221	3	49	theme	%	544:544	arg1	NaCl					583:586	60.9 % whey, 10 g/l soybean meal, and 2.9 % NaCl	539:586	60.9 % whey, 10 g/l soybean meal, and 2.9 % NaCl	539:586	To reduce the fermentation cost, an economical fermentation medium containing 60.9 % whey, 10 g/l soybean meal, and 2.9 % NaCl was developed.
24872221	4	50	theme	batch	620:624	arg1	fermentation					626:637	batch fermentation	620:637	batch fermentation	620:637	The EPS yield of batch fermentation in this medium reached 12.1 ± 0.3 g/l.
24872221	3	51	theme	10	552:553	arg1	g/l					555:557	g/l	555:557	g/l	555:557	To reduce the fermentation cost, an economical fermentation medium containing 60.9 % whey, 10 g/l soybean meal, and 2.9 % NaCl was developed.
24872221	1	52	theme	Many	151:154	arg1	microorganisms					163:176	Many marine microorganisms	151:176	Many marine microorganisms	151:176	Many marine microorganisms can secrete exopolysaccharides (EPSs) which have important applications in biotechnology.
24872221	4	53	theme	EPS	607:609	arg1	yield					611:615	The EPS yield	603:615	The EPS yield of batch fermentation in this medium	603:652	The EPS yield of batch fermentation in this medium reached 12.1 ± 0.3 g/l.
24872221	3	54	contain	containing	528:537	arg1	medium					521:526	an economical fermentation medium	494:526	an economical fermentation medium containing 60.9 % whey, 10 g/l soybean meal, and 2.9 % NaCl	494:586	To reduce the fermentation cost, an economical fermentation medium containing 60.9 % whey, 10 g/l soybean meal, and 2.9 % NaCl was developed.
24872221	3	54	contain	containing	528:537	arg2	NaCl					583:586	60.9 % whey, 10 g/l soybean meal, and 2.9 % NaCl	539:586	60.9 % whey, 10 g/l soybean meal, and 2.9 % NaCl	539:586	To reduce the fermentation cost, an economical fermentation medium containing 60.9 % whey, 10 g/l soybean meal, and 2.9 % NaCl was developed.
24872221	9	55	theme	SM-A87	1148:1153	arg1	EPS					1155:1157	SM-A87 EPS	1148:1157	SM-A87 EPS	1148:1157	These results suggest that SM-A87 EPS has promising potentials in biotechnology.
24872221	1	56	theme	marine	156:161	arg1	microorganisms					163:176	Many marine microorganisms	151:176	Many marine microorganisms	151:176	Many marine microorganisms can secrete exopolysaccharides (EPSs) which have important applications in biotechnology.
24872221	9	57	contain	has	1159:1161	arg2	potentials					1173:1182	promising potentials	1163:1182	promising potentials	1163:1182	These results suggest that SM-A87 EPS has promising potentials in biotechnology.
24872221	9	57	contain	has	1159:1161	arg1	EPS					1155:1157	SM-A87 EPS	1148:1157	SM-A87 EPS	1148:1157	These results suggest that SM-A87 EPS has promising potentials in biotechnology.
29220812	3	0	theme	residual	726:733	arg1	biomass					735:741	residual biomass	726:741	residual biomass	726:741	Cellulose content increased from 39.9% to a maximum of 89.3% w/w using treatments with 50% GVL at 156 °C for 0.5 h. Temperature had the most significant effect (RSM p < .05) on cellulose content of residual biomass and reducing operational duration of < 0.5 h may be viable according to RSM.
29220812	4	1	theme	theoretical	901:911	arg1	yield					921:925	up to 94% theoretical ethanol yield	891:925	up to 94% theoretical ethanol yield	891:925	PSSF fermentations of optimised pretreated eucalyptus sawdust produced up to 94% theoretical ethanol yield, which corresponded to approximately 181 kg of ethanol per dry ton of eucalyptus sawdust.
29220812	1	2	theme	sawdust	187:193	arg1	pretreatment					149:160	gamma-valerolactone (GVL) pretreatment	123:160	gamma-valerolactone (GVL) pretreatment of Australian eucalyptus sawdust for high cellulose biomass and bioethanol production	123:246	Optimisation of conditions for gamma-valerolactone (GVL) pretreatment of Australian eucalyptus sawdust for high cellulose biomass and bioethanol production was demonstrated.
29220812	3	3	theme	cellulose	705:713	arg1	content					715:721	cellulose content	705:721	cellulose content of residual biomass	705:741	Cellulose content increased from 39.9% to a maximum of 89.3% w/w using treatments with 50% GVL at 156 °C for 0.5 h. Temperature had the most significant effect (RSM p < .05) on cellulose content of residual biomass and reducing operational duration of < 0.5 h may be viable according to RSM.
29220812	2	4	theme	Optimum	410:416	arg1	conditions					418:427	0.5-2.0 h. Optimum conditions	399:427	0.5-2.0 h. Optimum conditions	399:427	Pretreatment parameters investigated included GVL concentrations of 35-50% w/w, temperatures of 120-180 °C and reaction durations of 0.5-2.0 h. Optimum conditions were determined using the response surface method (RSM) and central composite face-centred design.
29220812	4	5	theme	dry	986:988	arg1	ton					990:992	dry ton	986:992	dry ton of eucalyptus sawdust	986:1014	PSSF fermentations of optimised pretreated eucalyptus sawdust produced up to 94% theoretical ethanol yield, which corresponded to approximately 181 kg of ethanol per dry ton of eucalyptus sawdust.
29220812	4	6	theme	sawdust	874:880	arg1	fermentations					825:837	PSSF fermentations	820:837	PSSF fermentations of optimised pretreated eucalyptus sawdust	820:880	PSSF fermentations of optimised pretreated eucalyptus sawdust produced up to 94% theoretical ethanol yield, which corresponded to approximately 181 kg of ethanol per dry ton of eucalyptus sawdust.
29220812	3	7	theme	 w/w	588:591	arg1	maximum					572:578	a maximum	570:578	a maximum of 89.3% w/w using treatments with 50% GVL at 156 °C for 0.5 h. Temperature had the most significant effect (RSM p < .05) on cellulose content of residual biomass and reducing operational duration of < 0.5 h may be viable according to RSM	570:817	Cellulose content increased from 39.9% to a maximum of 89.3% w/w using treatments with 50% GVL at 156 °C for 0.5 h. Temperature had the most significant effect (RSM p < .05) on cellulose content of residual biomass and reducing operational duration of < 0.5 h may be viable according to RSM.
29220812	3	8	theme	50	615:616	arg1	%					617:617	%	617:617	%	617:617	Cellulose content increased from 39.9% to a maximum of 89.3% w/w using treatments with 50% GVL at 156 °C for 0.5 h. Temperature had the most significant effect (RSM p < .05) on cellulose content of residual biomass and reducing operational duration of < 0.5 h may be viable according to RSM.
29220812	2	9	theme	 w/w	340:343	arg1	concentrations					316:329	GVL concentrations	312:329	GVL concentrations of 35-50% w/w	312:343	Pretreatment parameters investigated included GVL concentrations of 35-50% w/w, temperatures of 120-180 °C and reaction durations of 0.5-2.0 h. Optimum conditions were determined using the response surface method (RSM) and central composite face-centred design.
29220812	2	10	theme	conditions	418:427	arg1	120-180 °C					362:371	120-180 °C	362:371	120-180 °C	362:371	Pretreatment parameters investigated included GVL concentrations of 35-50% w/w, temperatures of 120-180 °C and reaction durations of 0.5-2.0 h. Optimum conditions were determined using the response surface method (RSM) and central composite face-centred design.
29220812	2	10	theme	conditions	418:427	arg1	durations					386:394	reaction durations	377:394	reaction durations	377:394	Pretreatment parameters investigated included GVL concentrations of 35-50% w/w, temperatures of 120-180 °C and reaction durations of 0.5-2.0 h. Optimum conditions were determined using the response surface method (RSM) and central composite face-centred design.
29220812	2	11	theme	0.5-2.0 h.	399:408	arg1	conditions					418:427	0.5-2.0 h. Optimum conditions	399:427	0.5-2.0 h. Optimum conditions	399:427	Pretreatment parameters investigated included GVL concentrations of 35-50% w/w, temperatures of 120-180 °C and reaction durations of 0.5-2.0 h. Optimum conditions were determined using the response surface method (RSM) and central composite face-centred design.
29220812	3	12	with	treatments	599:608	arg1	GVL					619:621	50% GVL	615:621	50% GVL	615:621	Cellulose content increased from 39.9% to a maximum of 89.3% w/w using treatments with 50% GVL at 156 °C for 0.5 h. Temperature had the most significant effect (RSM p < .05) on cellulose content of residual biomass and reducing operational duration of < 0.5 h may be viable according to RSM.
29220812	2	13	theme	%	339:339	arg1	 w/w					340:343	35-50% w/w	334:343	35-50% w/w	334:343	Pretreatment parameters investigated included GVL concentrations of 35-50% w/w, temperatures of 120-180 °C and reaction durations of 0.5-2.0 h. Optimum conditions were determined using the response surface method (RSM) and central composite face-centred design.
29220812	1	14	theme	high	199:202	arg1	biomass					214:220	high cellulose biomass	199:220	high cellulose biomass	199:220	Optimisation of conditions for gamma-valerolactone (GVL) pretreatment of Australian eucalyptus sawdust for high cellulose biomass and bioethanol production was demonstrated.
29220812	3	15	theme	biomass	735:741	arg1	content					715:721	cellulose content	705:721	cellulose content of residual biomass	705:741	Cellulose content increased from 39.9% to a maximum of 89.3% w/w using treatments with 50% GVL at 156 °C for 0.5 h. Temperature had the most significant effect (RSM p < .05) on cellulose content of residual biomass and reducing operational duration of < 0.5 h may be viable according to RSM.
29220812	4	16	dep	yield	921:925	arg1	%					899:899	%	899:899	%	899:899	PSSF fermentations of optimised pretreated eucalyptus sawdust produced up to 94% theoretical ethanol yield, which corresponded to approximately 181 kg of ethanol per dry ton of eucalyptus sawdust.
29220812	2	17	theme	35-50	334:338	arg1	%					339:339	%	339:339	%	339:339	Pretreatment parameters investigated included GVL concentrations of 35-50% w/w, temperatures of 120-180 °C and reaction durations of 0.5-2.0 h. Optimum conditions were determined using the response surface method (RSM) and central composite face-centred design.
29220812	1	18	theme	cellulose	204:212	arg1	biomass					214:220	high cellulose biomass	199:220	high cellulose biomass	199:220	Optimisation of conditions for gamma-valerolactone (GVL) pretreatment of Australian eucalyptus sawdust for high cellulose biomass and bioethanol production was demonstrated.
29220812	2	19	theme	face-centred	507:518	arg1	design					520:525	central composite face-centred design	489:525	central composite face-centred design	489:525	Pretreatment parameters investigated included GVL concentrations of 35-50% w/w, temperatures of 120-180 °C and reaction durations of 0.5-2.0 h. Optimum conditions were determined using the response surface method (RSM) and central composite face-centred design.
29220812	1	20	theme	conditions	108:117	arg1	Optimisation					92:103	Optimisation	92:103	Optimisation of conditions for gamma-valerolactone (GVL) pretreatment of Australian eucalyptus sawdust for high cellulose biomass and bioethanol production	92:246	Optimisation of conditions for gamma-valerolactone (GVL) pretreatment of Australian eucalyptus sawdust for high cellulose biomass and bioethanol production was demonstrated.
29220812	3	21	theme	89.3	583:586	arg1	%					587:587	%	587:587	%	587:587	Cellulose content increased from 39.9% to a maximum of 89.3% w/w using treatments with 50% GVL at 156 °C for 0.5 h. Temperature had the most significant effect (RSM p < .05) on cellulose content of residual biomass and reducing operational duration of < 0.5 h may be viable according to RSM.
29220812	1	22	theme	biomass	214:220	arg1	production					237:246	high cellulose biomass and bioethanol production	199:246	high cellulose biomass and bioethanol production	199:246	Optimisation of conditions for gamma-valerolactone (GVL) pretreatment of Australian eucalyptus sawdust for high cellulose biomass and bioethanol production was demonstrated.
29220812	0	23	theme	Bioethanol	0:9	arg1	potential					11:19	Bioethanol potential	0:19	Bioethanol potential of Eucalyptus obliqua sawdust using gamma-valerolactone fractionation.	0:90	Bioethanol potential of Eucalyptus obliqua sawdust using gamma-valerolactone fractionation.
29220812	2	24	theme	120-180 °C	362:371	arg1	temperatures					346:357	temperatures	346:357	temperatures of 120-180 °C and reaction durations of 0.5-2.0 h. Optimum conditions	346:427	Pretreatment parameters investigated included GVL concentrations of 35-50% w/w, temperatures of 120-180 °C and reaction durations of 0.5-2.0 h. Optimum conditions were determined using the response surface method (RSM) and central composite face-centred design.
29220812	0	25	theme	Eucalyptus	24:33	arg1	sawdust					43:49	Eucalyptus obliqua sawdust	24:49	Eucalyptus obliqua sawdust using gamma-valerolactone fractionation	24:89	Bioethanol potential of Eucalyptus obliqua sawdust using gamma-valerolactone fractionation.
29220812	3	26	theme	0.5 h.	637:642	arg1	Temperature					644:654	0.5 h. Temperature	637:654	0.5 h. Temperature	637:654	Cellulose content increased from 39.9% to a maximum of 89.3% w/w using treatments with 50% GVL at 156 °C for 0.5 h. Temperature had the most significant effect (RSM p < .05) on cellulose content of residual biomass and reducing operational duration of < 0.5 h may be viable according to RSM.
29220812	5	27	theme	promising	1120:1128	arg1	solvent					1130:1136	a promising solvent	1118:1136	a promising solvent for lignocellulosic biorefining	1118:1168	The compositions of both the residual biomass and pretreatment liquors show that GVL pretreatment is a promising solvent for lignocellulosic biorefining.
29220812	5	27	theme	promising	1120:1128	arg1	pretreatment					1102:1113	GVL pretreatment	1098:1113	GVL pretreatment	1098:1113	The compositions of both the residual biomass and pretreatment liquors show that GVL pretreatment is a promising solvent for lignocellulosic biorefining.
29220812	5	28	theme	pretreatment	1067:1078	arg1	liquors					1080:1086	both the residual biomass and pretreatment liquors	1037:1086	liquors	1080:1086	The compositions of both the residual biomass and pretreatment liquors show that GVL pretreatment is a promising solvent for lignocellulosic biorefining.
29220812	1	29	theme	gamma-valerolactone	123:141	arg1	pretreatment					149:160	gamma-valerolactone (GVL) pretreatment	123:160	gamma-valerolactone (GVL) pretreatment of Australian eucalyptus sawdust for high cellulose biomass and bioethanol production	123:246	Optimisation of conditions for gamma-valerolactone (GVL) pretreatment of Australian eucalyptus sawdust for high cellulose biomass and bioethanol production was demonstrated.
29220812	5	30	theme	residual	1046:1053	arg1	biomass					1055:1061	residual biomass	1046:1061	residual biomass	1046:1061	The compositions of both the residual biomass and pretreatment liquors show that GVL pretreatment is a promising solvent for lignocellulosic biorefining.
29220812	1	31	theme	bioethanol	226:235	arg1	production					237:246	high cellulose biomass and bioethanol production	199:246	high cellulose biomass and bioethanol production	199:246	Optimisation of conditions for gamma-valerolactone (GVL) pretreatment of Australian eucalyptus sawdust for high cellulose biomass and bioethanol production was demonstrated.
29220812	3	32	theme	%	617:617	arg1	GVL					619:621	50% GVL	615:621	50% GVL	615:621	Cellulose content increased from 39.9% to a maximum of 89.3% w/w using treatments with 50% GVL at 156 °C for 0.5 h. Temperature had the most significant effect (RSM p < .05) on cellulose content of residual biomass and reducing operational duration of < 0.5 h may be viable according to RSM.
29220812	2	33	theme	composite	497:505	arg1	design					520:525	central composite face-centred design	489:525	central composite face-centred design	489:525	Pretreatment parameters investigated included GVL concentrations of 35-50% w/w, temperatures of 120-180 °C and reaction durations of 0.5-2.0 h. Optimum conditions were determined using the response surface method (RSM) and central composite face-centred design.
29220812	4	34	dep	94	897:898	arg1	to					894:895	to	894:895	to	894:895	PSSF fermentations of optimised pretreated eucalyptus sawdust produced up to 94% theoretical ethanol yield, which corresponded to approximately 181 kg of ethanol per dry ton of eucalyptus sawdust.
29220812	0	35	theme	sawdust	43:49	arg1	potential					11:19	Bioethanol potential	0:19	Bioethanol potential of Eucalyptus obliqua sawdust using gamma-valerolactone fractionation.	0:90	Bioethanol potential of Eucalyptus obliqua sawdust using gamma-valerolactone fractionation.
29220812	4	36	theme	ethanol	974:980	arg1	181 kg					964:969	approximately 181 kg	950:969	approximately 181 kg of ethanol per dry ton of eucalyptus sawdust	950:1014	PSSF fermentations of optimised pretreated eucalyptus sawdust produced up to 94% theoretical ethanol yield, which corresponded to approximately 181 kg of ethanol per dry ton of eucalyptus sawdust.
29220812	4	37	theme	pretreated	852:861	arg1	sawdust					874:880	optimised pretreated eucalyptus sawdust	842:880	optimised pretreated eucalyptus sawdust	842:880	PSSF fermentations of optimised pretreated eucalyptus sawdust produced up to 94% theoretical ethanol yield, which corresponded to approximately 181 kg of ethanol per dry ton of eucalyptus sawdust.
29220812	2	38	theme	surface	464:470	arg1	RSM					480:482	RSM	480:482	RSM	480:482	Pretreatment parameters investigated included GVL concentrations of 35-50% w/w, temperatures of 120-180 °C and reaction durations of 0.5-2.0 h. Optimum conditions were determined using the response surface method (RSM) and central composite face-centred design.
29220812	2	38	theme	surface	464:470	arg1	method					472:477	the response surface method	451:477	the response surface method (RSM)	451:483	Pretreatment parameters investigated included GVL concentrations of 35-50% w/w, temperatures of 120-180 °C and reaction durations of 0.5-2.0 h. Optimum conditions were determined using the response surface method (RSM) and central composite face-centred design.
29220812	0	39	theme	obliqua	35:41	arg1	sawdust					43:49	Eucalyptus obliqua sawdust	24:49	Eucalyptus obliqua sawdust using gamma-valerolactone fractionation	24:89	Bioethanol potential of Eucalyptus obliqua sawdust using gamma-valerolactone fractionation.
29220812	2	40	theme	central	489:495	arg1	design					520:525	central composite face-centred design	489:525	central composite face-centred design	489:525	Pretreatment parameters investigated included GVL concentrations of 35-50% w/w, temperatures of 120-180 °C and reaction durations of 0.5-2.0 h. Optimum conditions were determined using the response surface method (RSM) and central composite face-centred design.
29220812	0	41	theme	gamma-valerolactone	57:75	arg1	fractionation					77:89	gamma-valerolactone fractionation	57:89	gamma-valerolactone fractionation	57:89	Bioethanol potential of Eucalyptus obliqua sawdust using gamma-valerolactone fractionation.
29220812	3	42	theme	significant	669:679	arg1	effect					681:686	the most significant effect	660:686	the most significant effect (RSM p < .05)	660:700	Cellulose content increased from 39.9% to a maximum of 89.3% w/w using treatments with 50% GVL at 156 °C for 0.5 h. Temperature had the most significant effect (RSM p < .05) on cellulose content of residual biomass and reducing operational duration of < 0.5 h may be viable according to RSM.
29220812	3	42	theme	significant	669:679	arg1	RSM					689:691	RSM p < .05	689:699	RSM p < .05	689:699	Cellulose content increased from 39.9% to a maximum of 89.3% w/w using treatments with 50% GVL at 156 °C for 0.5 h. Temperature had the most significant effect (RSM p < .05) on cellulose content of residual biomass and reducing operational duration of < 0.5 h may be viable according to RSM.
29220812	2	43	theme	Pretreatment	266:277	arg1	parameters					279:288	Pretreatment parameters	266:288	Pretreatment parameters investigated	266:301	Pretreatment parameters investigated included GVL concentrations of 35-50% w/w, temperatures of 120-180 °C and reaction durations of 0.5-2.0 h. Optimum conditions were determined using the response surface method (RSM) and central composite face-centred design.
29220812	5	44	theme	lignocellulosic	1142:1156	arg1	biorefining					1158:1168	lignocellulosic biorefining	1142:1168	lignocellulosic biorefining	1142:1168	The compositions of both the residual biomass and pretreatment liquors show that GVL pretreatment is a promising solvent for lignocellulosic biorefining.
29220812	1	45	theme	GVL	144:146	arg1	pretreatment					149:160	gamma-valerolactone (GVL) pretreatment	123:160	gamma-valerolactone (GVL) pretreatment of Australian eucalyptus sawdust for high cellulose biomass and bioethanol production	123:246	Optimisation of conditions for gamma-valerolactone (GVL) pretreatment of Australian eucalyptus sawdust for high cellulose biomass and bioethanol production was demonstrated.
29220812	4	46	dep	%	899:899	arg1	94					897:898	94	897:898	94	897:898	PSSF fermentations of optimised pretreated eucalyptus sawdust produced up to 94% theoretical ethanol yield, which corresponded to approximately 181 kg of ethanol per dry ton of eucalyptus sawdust.
29220812	5	47	theme	liquors	1080:1086	arg1	compositions					1021:1032	The compositions	1017:1032	The compositions of both the residual biomass and pretreatment liquors	1017:1086	The compositions of both the residual biomass and pretreatment liquors show that GVL pretreatment is a promising solvent for lignocellulosic biorefining.
29220812	4	48	theme	eucalyptus	863:872	arg1	sawdust					874:880	optimised pretreated eucalyptus sawdust	842:880	optimised pretreated eucalyptus sawdust	842:880	PSSF fermentations of optimised pretreated eucalyptus sawdust produced up to 94% theoretical ethanol yield, which corresponded to approximately 181 kg of ethanol per dry ton of eucalyptus sawdust.
29220812	3	49	theme	operational	756:766	arg1	duration					768:775	operational duration	756:775	operational duration of < 0.5 h may be viable according to RSM	756:817	Cellulose content increased from 39.9% to a maximum of 89.3% w/w using treatments with 50% GVL at 156 °C for 0.5 h. Temperature had the most significant effect (RSM p < .05) on cellulose content of residual biomass and reducing operational duration of < 0.5 h may be viable according to RSM.
29220812	5	50	theme	biomass	1055:1061	arg1	liquors					1080:1086	both the residual biomass and pretreatment liquors	1037:1086	liquors	1080:1086	The compositions of both the residual biomass and pretreatment liquors show that GVL pretreatment is a promising solvent for lignocellulosic biorefining.
29220812	4	51	theme	PSSF	820:823	arg1	fermentations					825:837	PSSF fermentations	820:837	PSSF fermentations of optimised pretreated eucalyptus sawdust	820:880	PSSF fermentations of optimised pretreated eucalyptus sawdust produced up to 94% theoretical ethanol yield, which corresponded to approximately 181 kg of ethanol per dry ton of eucalyptus sawdust.
29220812	4	52	theme	optimised	842:850	arg1	sawdust					874:880	optimised pretreated eucalyptus sawdust	842:880	optimised pretreated eucalyptus sawdust	842:880	PSSF fermentations of optimised pretreated eucalyptus sawdust produced up to 94% theoretical ethanol yield, which corresponded to approximately 181 kg of ethanol per dry ton of eucalyptus sawdust.
29220812	2	53	theme	durations	386:394	arg1	temperatures					346:357	temperatures	346:357	temperatures of 120-180 °C and reaction durations of 0.5-2.0 h. Optimum conditions	346:427	Pretreatment parameters investigated included GVL concentrations of 35-50% w/w, temperatures of 120-180 °C and reaction durations of 0.5-2.0 h. Optimum conditions were determined using the response surface method (RSM) and central composite face-centred design.
29220812	3	54	theme	%	587:587	arg1	 w/w					588:591	89.3% w/w	583:591	89.3% w/w using treatments with 50% GVL at 156 °C for 0.5 h. Temperature had the most significant effect (RSM p < .05) on cellulose content of residual biomass and reducing operational duration of < 0.5 h may be viable according to RSM	583:817	Cellulose content increased from 39.9% to a maximum of 89.3% w/w using treatments with 50% GVL at 156 °C for 0.5 h. Temperature had the most significant effect (RSM p < .05) on cellulose content of residual biomass and reducing operational duration of < 0.5 h may be viable according to RSM.
29220812	2	55	theme	reaction	377:384	arg1	durations					386:394	reaction durations	377:394	reaction durations	377:394	Pretreatment parameters investigated included GVL concentrations of 35-50% w/w, temperatures of 120-180 °C and reaction durations of 0.5-2.0 h. Optimum conditions were determined using the response surface method (RSM) and central composite face-centred design.
29220812	4	56	theme	ethanol	913:919	arg1	yield					921:925	up to 94% theoretical ethanol yield	891:925	up to 94% theoretical ethanol yield	891:925	PSSF fermentations of optimised pretreated eucalyptus sawdust produced up to 94% theoretical ethanol yield, which corresponded to approximately 181 kg of ethanol per dry ton of eucalyptus sawdust.
29220812	4	57	theme	sawdust	1008:1014	arg1	ton					990:992	dry ton	986:992	dry ton of eucalyptus sawdust	986:1014	PSSF fermentations of optimised pretreated eucalyptus sawdust produced up to 94% theoretical ethanol yield, which corresponded to approximately 181 kg of ethanol per dry ton of eucalyptus sawdust.
29220812	5	58	theme	GVL	1098:1100	arg1	solvent					1130:1136	a promising solvent	1118:1136	a promising solvent for lignocellulosic biorefining	1118:1168	The compositions of both the residual biomass and pretreatment liquors show that GVL pretreatment is a promising solvent for lignocellulosic biorefining.
29220812	5	58	theme	GVL	1098:1100	arg1	pretreatment					1102:1113	GVL pretreatment	1098:1113	GVL pretreatment	1098:1113	The compositions of both the residual biomass and pretreatment liquors show that GVL pretreatment is a promising solvent for lignocellulosic biorefining.
29220812	2	59	theme	GVL	312:314	arg1	concentrations					316:329	GVL concentrations	312:329	GVL concentrations of 35-50% w/w	312:343	Pretreatment parameters investigated included GVL concentrations of 35-50% w/w, temperatures of 120-180 °C and reaction durations of 0.5-2.0 h. Optimum conditions were determined using the response surface method (RSM) and central composite face-centred design.
29220812	3	60	theme	Cellulose	528:536	arg1	content					538:544	Cellulose content	528:544	Cellulose content	528:544	Cellulose content increased from 39.9% to a maximum of 89.3% w/w using treatments with 50% GVL at 156 °C for 0.5 h. Temperature had the most significant effect (RSM p < .05) on cellulose content of residual biomass and reducing operational duration of < 0.5 h may be viable according to RSM.
29220812	1	61	theme	Australian	165:174	arg1	sawdust					187:193	Australian eucalyptus sawdust	165:193	Australian eucalyptus sawdust	165:193	Optimisation of conditions for gamma-valerolactone (GVL) pretreatment of Australian eucalyptus sawdust for high cellulose biomass and bioethanol production was demonstrated.
29220812	2	62	theme	response	455:462	arg1	RSM					480:482	RSM	480:482	RSM	480:482	Pretreatment parameters investigated included GVL concentrations of 35-50% w/w, temperatures of 120-180 °C and reaction durations of 0.5-2.0 h. Optimum conditions were determined using the response surface method (RSM) and central composite face-centred design.
29220812	2	62	theme	response	455:462	arg1	method					472:477	the response surface method	451:477	the response surface method (RSM)	451:483	Pretreatment parameters investigated included GVL concentrations of 35-50% w/w, temperatures of 120-180 °C and reaction durations of 0.5-2.0 h. Optimum conditions were determined using the response surface method (RSM) and central composite face-centred design.
29220812	4	63	theme	eucalyptus	997:1006	arg1	sawdust					1008:1014	eucalyptus sawdust	997:1014	eucalyptus sawdust	997:1014	PSSF fermentations of optimised pretreated eucalyptus sawdust produced up to 94% theoretical ethanol yield, which corresponded to approximately 181 kg of ethanol per dry ton of eucalyptus sawdust.
29220812	4	64	dep	produced	882:889	arg1	corresponded					934:945	corresponded	934:945	corresponded to approximately 181 kg of ethanol per dry ton of eucalyptus sawdust	934:1014	PSSF fermentations of optimised pretreated eucalyptus sawdust produced up to 94% theoretical ethanol yield, which corresponded to approximately 181 kg of ethanol per dry ton of eucalyptus sawdust.
29220812	1	65	theme	eucalyptus	176:185	arg1	sawdust					187:193	Australian eucalyptus sawdust	165:193	Australian eucalyptus sawdust	165:193	Optimisation of conditions for gamma-valerolactone (GVL) pretreatment of Australian eucalyptus sawdust for high cellulose biomass and bioethanol production was demonstrated.
29220812	3	66	contain	had	656:658	arg1	Temperature					644:654	0.5 h. Temperature	637:654	0.5 h. Temperature	637:654	Cellulose content increased from 39.9% to a maximum of 89.3% w/w using treatments with 50% GVL at 156 °C for 0.5 h. Temperature had the most significant effect (RSM p < .05) on cellulose content of residual biomass and reducing operational duration of < 0.5 h may be viable according to RSM.
29220812	3	66	contain	had	656:658	arg2	RSM					689:691	RSM p < .05	689:699	RSM p < .05	689:699	Cellulose content increased from 39.9% to a maximum of 89.3% w/w using treatments with 50% GVL at 156 °C for 0.5 h. Temperature had the most significant effect (RSM p < .05) on cellulose content of residual biomass and reducing operational duration of < 0.5 h may be viable according to RSM.
29220812	3	66	contain	had	656:658	arg2	effect					681:686	the most significant effect	660:686	the most significant effect (RSM p < .05)	660:700	Cellulose content increased from 39.9% to a maximum of 89.3% w/w using treatments with 50% GVL at 156 °C for 0.5 h. Temperature had the most significant effect (RSM p < .05) on cellulose content of residual biomass and reducing operational duration of < 0.5 h may be viable according to RSM.
23828181	1	0	theme	%	310:310	arg1	solubilization					312:325	80% solubilization	308:325	80% solubilization	308:325	Morphology, molecular structure, and thermal properties of potato starch granules with low to high phosphate content were studied as an effect of mild acid hydrolysis (lintnerization) to 80% solubilization at two temperatures (25 and 45°C).
23828181	6	1	theme	phosphate	1031:1039	arg1	content					1041:1047	the phosphate content	1027:1047	an altered amylopectin structure rather than the phosphate content	982:1047	High-phosphate-containing starch was, however, unaffected by temperature and this was probably due to an altered amylopectin structure rather than the phosphate content.
23828181	0	2	theme	molecular	72:80	arg1	composition					82:92	the molecular composition	68:92	the molecular composition of potato starch lintners	68:118	Influence of amylopectin structure and degree of phosphorylation on the molecular composition of potato starch lintners.
23828181	5	3	contain	contained	768:776	arg2	more					778:781	more	778:781	more	778:781	When lintnerization was performed at 45°C, the lintners contained more of branched dextrins compared to 25°C in both low and intermediate phosphate-containing samples.
23828181	5	3	contain	contained	768:776	arg1	lintners					759:766	the lintners	755:766	the lintners	755:766	When lintnerization was performed at 45°C, the lintners contained more of branched dextrins compared to 25°C in both low and intermediate phosphate-containing samples.
23828181	1	4	theme	thermal	158:164	arg1	properties					166:175	thermal properties	158:175	thermal properties of potato starch granules with low to high phosphate content	158:236	Morphology, molecular structure, and thermal properties of potato starch granules with low to high phosphate content were studied as an effect of mild acid hydrolysis (lintnerization) to 80% solubilization at two temperatures (25 and 45°C).
23828181	1	4	theme	thermal	158:164	arg1	Morphology					121:130	Morphology	121:130	Morphology	121:130	Morphology, molecular structure, and thermal properties of potato starch granules with low to high phosphate content were studied as an effect of mild acid hydrolysis (lintnerization) to 80% solubilization at two temperatures (25 and 45°C).
23828181	1	4	theme	thermal	158:164	arg1	structure					143:151	molecular structure	133:151	molecular structure	133:151	Morphology, molecular structure, and thermal properties of potato starch granules with low to high phosphate content were studied as an effect of mild acid hydrolysis (lintnerization) to 80% solubilization at two temperatures (25 and 45°C).
23828181	1	4	theme	thermal	158:164	arg1	effect					257:262	an effect	254:262	an effect of mild acid hydrolysis (lintnerization) to 80% solubilization	254:325	Morphology, molecular structure, and thermal properties of potato starch granules with low to high phosphate content were studied as an effect of mild acid hydrolysis (lintnerization) to 80% solubilization at two temperatures (25 and 45°C).
23828181	0	5	theme	potato	97:102	arg1	lintners					111:118	potato starch lintners	97:118	potato starch lintners	97:118	Influence of amylopectin structure and degree of phosphorylation on the molecular composition of potato starch lintners.
23828181	0	6	from	Influence	0:8	arg1	composition					82:92	the molecular composition	68:92	the molecular composition of potato starch lintners	68:118	Influence of amylopectin structure and degree of phosphorylation on the molecular composition of potato starch lintners.
23828181	7	7	theme	melting	1146:1152	arg1	temperatures					1154:1165	increased peak melting temperatures	1131:1165	increased peak melting temperatures	1131:1165	After lintnerization, the melting endotherms were broad with decreased onset and increased peak melting temperatures.
23828181	3	8	theme	rehydrated	531:540	arg1	lintners					542:549	rehydrated lintners	531:549	rehydrated lintners	531:549	Transmission electron microscopy of rehydrated lintners revealed lacy networks of smaller subunits.
23828181	2	9	contain	contained	404:412	arg2	granules					432:439	apparently intact granules	414:439	apparently intact granules	414:439	Light microscopy showed that the lintners contained apparently intact granules, which disintegrated into fragments upon dehydration.
23828181	2	9	contain	contained	404:412	arg1	lintners					395:402	the lintners	391:402	the lintners	391:402	Light microscopy showed that the lintners contained apparently intact granules, which disintegrated into fragments upon dehydration.
23828181	3	10	theme	lintners	542:549	arg1	microscopy					517:526	Transmission electron microscopy	495:526	Transmission electron microscopy of rehydrated lintners	495:549	Transmission electron microscopy of rehydrated lintners revealed lacy networks of smaller subunits.
23828181	0	11	theme	lintners	111:118	arg1	composition					82:92	the molecular composition	68:92	the molecular composition of potato starch lintners	68:118	Influence of amylopectin structure and degree of phosphorylation on the molecular composition of potato starch lintners.
23828181	5	12	theme	intermediate	837:848	arg1	samples					871:877	both low and intermediate phosphate-containing samples	824:877	both low and intermediate phosphate-containing samples	824:877	When lintnerization was performed at 45°C, the lintners contained more of branched dextrins compared to 25°C in both low and intermediate phosphate-containing samples.
23828181	4	13	theme	lintners	628:635	arg1	composition					609:619	The molecular composition	595:619	The molecular composition of the lintners	595:635	The molecular composition of the lintners suggested that they largely consisted of remnants of crystalline lamellae.
23828181	7	14	with	broad	1100:1104	arg1	temperatures					1154:1165	increased peak melting temperatures	1131:1165	increased peak melting temperatures	1131:1165	After lintnerization, the melting endotherms were broad with decreased onset and increased peak melting temperatures.
23828181	7	14	with	broad	1100:1104	arg1	onset					1121:1125	decreased onset	1111:1125	decreased onset	1111:1125	After lintnerization, the melting endotherms were broad with decreased onset and increased peak melting temperatures.
23828181	0	15	theme	starch	104:109	arg1	lintners					111:118	potato starch lintners	97:118	potato starch lintners	97:118	Influence of amylopectin structure and degree of phosphorylation on the molecular composition of potato starch lintners.
23828181	5	16	from	25°C	816:819	arg1	samples					871:877	both low and intermediate phosphate-containing samples	824:877	both low and intermediate phosphate-containing samples	824:877	When lintnerization was performed at 45°C, the lintners contained more of branched dextrins compared to 25°C in both low and intermediate phosphate-containing samples.
23828181	5	17	theme	branched	786:793	arg1	dextrins					795:802	branched dextrins	786:802	branched dextrins	786:802	When lintnerization was performed at 45°C, the lintners contained more of branched dextrins compared to 25°C in both low and intermediate phosphate-containing samples.
23828181	9	18	theme	lintnerization	1278:1291	arg1	kinetics					1266:1273	the kinetics	1262:1273	the kinetics of lintnerization	1262:1291	A hypothesis that combines the kinetics of lintnerization with the molecular and thermal characteristics of the lintners is presented.
23828181	4	19	theme	molecular	599:607	arg1	composition					609:619	The molecular composition	595:619	The molecular composition of the lintners	595:635	The molecular composition of the lintners suggested that they largely consisted of remnants of crystalline lamellae.
23828181	1	20	theme	potato	180:185	arg1	granules					194:201	potato starch granules	180:201	potato starch granules with low to high phosphate content	180:236	Morphology, molecular structure, and thermal properties of potato starch granules with low to high phosphate content were studied as an effect of mild acid hydrolysis (lintnerization) to 80% solubilization at two temperatures (25 and 45°C).
23828181	9	21	theme	lintners	1347:1354	arg1	characteristics					1324:1338	the molecular and thermal characteristics	1298:1338	the molecular and thermal characteristics of the lintners	1298:1354	A hypothesis that combines the kinetics of lintnerization with the molecular and thermal characteristics of the lintners is presented.
23828181	7	22	theme	increased	1131:1139	arg1	temperatures					1154:1165	increased peak melting temperatures	1131:1165	increased peak melting temperatures	1131:1165	After lintnerization, the melting endotherms were broad with decreased onset and increased peak melting temperatures.
23828181	1	23	theme	starch	187:192	arg1	granules					194:201	potato starch granules	180:201	potato starch granules with low to high phosphate content	180:236	Morphology, molecular structure, and thermal properties of potato starch granules with low to high phosphate content were studied as an effect of mild acid hydrolysis (lintnerization) to 80% solubilization at two temperatures (25 and 45°C).
23828181	0	24	theme	structure	25:33	arg1	Influence					0:8	Influence	0:8	Influence of amylopectin structure and degree of phosphorylation on the molecular composition of potato starch lintners.	0:119	Influence of amylopectin structure and degree of phosphorylation on the molecular composition of potato starch lintners.
23828181	1	25	theme	mild	267:270	arg1	lintnerization					289:302	lintnerization	289:302	lintnerization	289:302	Morphology, molecular structure, and thermal properties of potato starch granules with low to high phosphate content were studied as an effect of mild acid hydrolysis (lintnerization) to 80% solubilization at two temperatures (25 and 45°C).
23828181	1	25	theme	mild	267:270	arg1	hydrolysis					277:286	mild acid hydrolysis	267:286	mild acid hydrolysis (lintnerization)	267:303	Morphology, molecular structure, and thermal properties of potato starch granules with low to high phosphate content were studied as an effect of mild acid hydrolysis (lintnerization) to 80% solubilization at two temperatures (25 and 45°C).
23828181	6	26	theme	altered	985:991	arg1	this					957:960	this	957:960	this	957:960	High-phosphate-containing starch was, however, unaffected by temperature and this was probably due to an altered amylopectin structure rather than the phosphate content.
23828181	6	26	theme	altered	985:991	arg1	structure					1005:1013	an altered amylopectin structure	982:1013	an altered amylopectin structure rather than the phosphate content	982:1047	High-phosphate-containing starch was, however, unaffected by temperature and this was probably due to an altered amylopectin structure rather than the phosphate content.
23828181	7	27	theme	peak	1141:1144	arg1	temperatures					1154:1165	increased peak melting temperatures	1131:1165	increased peak melting temperatures	1131:1165	After lintnerization, the melting endotherms were broad with decreased onset and increased peak melting temperatures.
23828181	1	28	theme	granules	194:201	arg1	properties					166:175	thermal properties	158:175	thermal properties of potato starch granules with low to high phosphate content	158:236	Morphology, molecular structure, and thermal properties of potato starch granules with low to high phosphate content were studied as an effect of mild acid hydrolysis (lintnerization) to 80% solubilization at two temperatures (25 and 45°C).
23828181	1	28	theme	granules	194:201	arg1	Morphology					121:130	Morphology	121:130	Morphology	121:130	Morphology, molecular structure, and thermal properties of potato starch granules with low to high phosphate content were studied as an effect of mild acid hydrolysis (lintnerization) to 80% solubilization at two temperatures (25 and 45°C).
23828181	1	28	theme	granules	194:201	arg1	structure					143:151	molecular structure	133:151	molecular structure	133:151	Morphology, molecular structure, and thermal properties of potato starch granules with low to high phosphate content were studied as an effect of mild acid hydrolysis (lintnerization) to 80% solubilization at two temperatures (25 and 45°C).
23828181	1	28	theme	granules	194:201	arg1	effect					257:262	an effect	254:262	an effect of mild acid hydrolysis (lintnerization) to 80% solubilization	254:325	Morphology, molecular structure, and thermal properties of potato starch granules with low to high phosphate content were studied as an effect of mild acid hydrolysis (lintnerization) to 80% solubilization at two temperatures (25 and 45°C).
23828181	0	29	theme	amylopectin	13:23	arg1	structure					25:33	amylopectin structure	13:33	amylopectin structure	13:33	Influence of amylopectin structure and degree of phosphorylation on the molecular composition of potato starch lintners.
23828181	1	30	theme	acid	272:275	arg1	lintnerization					289:302	lintnerization	289:302	lintnerization	289:302	Morphology, molecular structure, and thermal properties of potato starch granules with low to high phosphate content were studied as an effect of mild acid hydrolysis (lintnerization) to 80% solubilization at two temperatures (25 and 45°C).
23828181	1	30	theme	acid	272:275	arg1	hydrolysis					277:286	mild acid hydrolysis	267:286	mild acid hydrolysis (lintnerization)	267:303	Morphology, molecular structure, and thermal properties of potato starch granules with low to high phosphate content were studied as an effect of mild acid hydrolysis (lintnerization) to 80% solubilization at two temperatures (25 and 45°C).
23828181	7	31	theme	decreased	1111:1119	arg1	onset					1121:1125	decreased onset	1111:1125	decreased onset	1111:1125	After lintnerization, the melting endotherms were broad with decreased onset and increased peak melting temperatures.
23828181	3	32	theme	lacy	560:563	arg1	networks					565:572	lacy networks	560:572	lacy networks of smaller subunits	560:592	Transmission electron microscopy of rehydrated lintners revealed lacy networks of smaller subunits.
23828181	4	33	theme	crystalline	690:700	arg1	lamellae					702:709	crystalline lamellae	690:709	crystalline lamellae	690:709	The molecular composition of the lintners suggested that they largely consisted of remnants of crystalline lamellae.
23828181	0	34	theme	degree	39:44	arg1	Influence					0:8	Influence	0:8	Influence of amylopectin structure and degree of phosphorylation on the molecular composition of potato starch lintners.	0:119	Influence of amylopectin structure and degree of phosphorylation on the molecular composition of potato starch lintners.
23828181	1	35	theme	hydrolysis	277:286	arg1	properties					166:175	thermal properties	158:175	thermal properties of potato starch granules with low to high phosphate content	158:236	Morphology, molecular structure, and thermal properties of potato starch granules with low to high phosphate content were studied as an effect of mild acid hydrolysis (lintnerization) to 80% solubilization at two temperatures (25 and 45°C).
23828181	1	35	theme	hydrolysis	277:286	arg1	Morphology					121:130	Morphology	121:130	Morphology	121:130	Morphology, molecular structure, and thermal properties of potato starch granules with low to high phosphate content were studied as an effect of mild acid hydrolysis (lintnerization) to 80% solubilization at two temperatures (25 and 45°C).
23828181	1	35	theme	hydrolysis	277:286	arg1	structure					143:151	molecular structure	133:151	molecular structure	133:151	Morphology, molecular structure, and thermal properties of potato starch granules with low to high phosphate content were studied as an effect of mild acid hydrolysis (lintnerization) to 80% solubilization at two temperatures (25 and 45°C).
23828181	1	35	theme	hydrolysis	277:286	arg1	effect					257:262	an effect	254:262	an effect of mild acid hydrolysis (lintnerization) to 80% solubilization	254:325	Morphology, molecular structure, and thermal properties of potato starch granules with low to high phosphate content were studied as an effect of mild acid hydrolysis (lintnerization) to 80% solubilization at two temperatures (25 and 45°C).
23828181	1	36	with	granules	194:201	arg1	content					230:236	low to high phosphate content	208:236	low to high phosphate content	208:236	Morphology, molecular structure, and thermal properties of potato starch granules with low to high phosphate content were studied as an effect of mild acid hydrolysis (lintnerization) to 80% solubilization at two temperatures (25 and 45°C).
23828181	5	37	theme	phosphate-containing	850:869	arg1	samples					871:877	both low and intermediate phosphate-containing samples	824:877	both low and intermediate phosphate-containing samples	824:877	When lintnerization was performed at 45°C, the lintners contained more of branched dextrins compared to 25°C in both low and intermediate phosphate-containing samples.
23828181	1	38	theme	low	208:210	arg1	content					230:236	low to high phosphate content	208:236	low to high phosphate content	208:236	Morphology, molecular structure, and thermal properties of potato starch granules with low to high phosphate content were studied as an effect of mild acid hydrolysis (lintnerization) to 80% solubilization at two temperatures (25 and 45°C).
23828181	6	39	theme	High-phosphate-containing	880:904	arg1	starch					906:911	High-phosphate-containing starch	880:911	High-phosphate-containing starch	880:911	High-phosphate-containing starch was, however, unaffected by temperature and this was probably due to an altered amylopectin structure rather than the phosphate content.
23828181	2	40	theme	Light	362:366	arg1	microscopy					368:377	Light microscopy	362:377	Light microscopy	362:377	Light microscopy showed that the lintners contained apparently intact granules, which disintegrated into fragments upon dehydration.
23828181	0	41	theme	phosphorylation	49:63	arg1	degree					39:44	degree	39:44	degree of phosphorylation	39:63	Influence of amylopectin structure and degree of phosphorylation on the molecular composition of potato starch lintners.
23828181	0	41	theme	phosphorylation	49:63	arg1	structure					25:33	amylopectin structure	13:33	amylopectin structure	13:33	Influence of amylopectin structure and degree of phosphorylation on the molecular composition of potato starch lintners.
23828181	7	42	theme	melting	1076:1082	arg1	broad					1100:1104	broad	1100:1104	broad	1100:1104	After lintnerization, the melting endotherms were broad with decreased onset and increased peak melting temperatures.
23828181	7	42	theme	melting	1076:1082	arg1	endotherms					1084:1093	the melting endotherms	1072:1093	the melting endotherms	1072:1093	After lintnerization, the melting endotherms were broad with decreased onset and increased peak melting temperatures.
23828181	6	43	theme	amylopectin	993:1003	arg1	this					957:960	this	957:960	this	957:960	High-phosphate-containing starch was, however, unaffected by temperature and this was probably due to an altered amylopectin structure rather than the phosphate content.
23828181	6	43	theme	amylopectin	993:1003	arg1	structure					1005:1013	an altered amylopectin structure	982:1013	an altered amylopectin structure rather than the phosphate content	982:1047	High-phosphate-containing starch was, however, unaffected by temperature and this was probably due to an altered amylopectin structure rather than the phosphate content.
23828181	3	44	theme	smaller	577:583	arg1	subunits					585:592	smaller subunits	577:592	smaller subunits	577:592	Transmission electron microscopy of rehydrated lintners revealed lacy networks of smaller subunits.
23828181	2	45	theme	intact	425:430	arg1	granules					432:439	apparently intact granules	414:439	apparently intact granules	414:439	Light microscopy showed that the lintners contained apparently intact granules, which disintegrated into fragments upon dehydration.
23828181	9	46	theme	molecular	1302:1310	arg1	characteristics					1324:1338	the molecular and thermal characteristics	1298:1338	the molecular and thermal characteristics of the lintners	1298:1354	A hypothesis that combines the kinetics of lintnerization with the molecular and thermal characteristics of the lintners is presented.
23828181	3	47	theme	subunits	585:592	arg1	networks					565:572	lacy networks	560:572	lacy networks of smaller subunits	560:592	Transmission electron microscopy of rehydrated lintners revealed lacy networks of smaller subunits.
23828181	1	48	dep	temperatures	334:345	arg1	45°C					355:358	45°C	355:358	45°C	355:358	Morphology, molecular structure, and thermal properties of potato starch granules with low to high phosphate content were studied as an effect of mild acid hydrolysis (lintnerization) to 80% solubilization at two temperatures (25 and 45°C).
23828181	1	48	dep	temperatures	334:345	arg1	25					348:349	25	348:349	25	348:349	Morphology, molecular structure, and thermal properties of potato starch granules with low to high phosphate content were studied as an effect of mild acid hydrolysis (lintnerization) to 80% solubilization at two temperatures (25 and 45°C).
23828181	1	49	dep	low	208:210	arg1	high					215:218	high	215:218	high	215:218	Morphology, molecular structure, and thermal properties of potato starch granules with low to high phosphate content were studied as an effect of mild acid hydrolysis (lintnerization) to 80% solubilization at two temperatures (25 and 45°C).
23828181	1	49	dep	low	208:210	arg1	to					212:213	to	212:213	to	212:213	Morphology, molecular structure, and thermal properties of potato starch granules with low to high phosphate content were studied as an effect of mild acid hydrolysis (lintnerization) to 80% solubilization at two temperatures (25 and 45°C).
23828181	4	50	theme	lamellae	702:709	arg1	remnants					678:685	remnants	678:685	remnants of crystalline lamellae	678:709	The molecular composition of the lintners suggested that they largely consisted of remnants of crystalline lamellae.
23828181	9	51	theme	thermal	1316:1322	arg1	characteristics					1324:1338	the molecular and thermal characteristics	1298:1338	the molecular and thermal characteristics of the lintners	1298:1354	A hypothesis that combines the kinetics of lintnerization with the molecular and thermal characteristics of the lintners is presented.
23828181	1	52	theme	molecular	133:141	arg1	properties					166:175	thermal properties	158:175	thermal properties of potato starch granules with low to high phosphate content	158:236	Morphology, molecular structure, and thermal properties of potato starch granules with low to high phosphate content were studied as an effect of mild acid hydrolysis (lintnerization) to 80% solubilization at two temperatures (25 and 45°C).
23828181	1	52	theme	molecular	133:141	arg1	Morphology					121:130	Morphology	121:130	Morphology	121:130	Morphology, molecular structure, and thermal properties of potato starch granules with low to high phosphate content were studied as an effect of mild acid hydrolysis (lintnerization) to 80% solubilization at two temperatures (25 and 45°C).
23828181	1	52	theme	molecular	133:141	arg1	structure					143:151	molecular structure	133:151	molecular structure	133:151	Morphology, molecular structure, and thermal properties of potato starch granules with low to high phosphate content were studied as an effect of mild acid hydrolysis (lintnerization) to 80% solubilization at two temperatures (25 and 45°C).
23828181	1	52	theme	molecular	133:141	arg1	effect					257:262	an effect	254:262	an effect of mild acid hydrolysis (lintnerization) to 80% solubilization	254:325	Morphology, molecular structure, and thermal properties of potato starch granules with low to high phosphate content were studied as an effect of mild acid hydrolysis (lintnerization) to 80% solubilization at two temperatures (25 and 45°C).
23828181	1	53	theme	phosphate	220:228	arg1	content					230:236	low to high phosphate content	208:236	low to high phosphate content	208:236	Morphology, molecular structure, and thermal properties of potato starch granules with low to high phosphate content were studied as an effect of mild acid hydrolysis (lintnerization) to 80% solubilization at two temperatures (25 and 45°C).
23828181	3	54	theme	Transmission	495:506	arg1	microscopy					517:526	Transmission electron microscopy	495:526	Transmission electron microscopy of rehydrated lintners	495:549	Transmission electron microscopy of rehydrated lintners revealed lacy networks of smaller subunits.
23828181	8	55	theme	relative	1172:1179	arg1	lower					1199:1203	lower	1199:1203	lower	1199:1203	The relative crystallinity was lower in lintners prepared at 45°C.
23828181	8	55	theme	relative	1172:1179	arg1	crystallinity					1181:1193	The relative crystallinity	1168:1193	The relative crystallinity	1168:1193	The relative crystallinity was lower in lintners prepared at 45°C.
23828181	1	56	theme	80	308:309	arg1	%					310:310	%	310:310	%	310:310	Morphology, molecular structure, and thermal properties of potato starch granules with low to high phosphate content were studied as an effect of mild acid hydrolysis (lintnerization) to 80% solubilization at two temperatures (25 and 45°C).
23828181	5	57	theme	low	829:831	arg1	samples					871:877	both low and intermediate phosphate-containing samples	824:877	both low and intermediate phosphate-containing samples	824:877	When lintnerization was performed at 45°C, the lintners contained more of branched dextrins compared to 25°C in both low and intermediate phosphate-containing samples.
23828181	3	58	theme	electron	508:515	arg1	microscopy					517:526	Transmission electron microscopy	495:526	Transmission electron microscopy of rehydrated lintners	495:549	Transmission electron microscopy of rehydrated lintners revealed lacy networks of smaller subunits.
24464238	0	0	theme	probiotic	78:86	arg1	microorganisms					110:123	probiotic Lactobacillus reuteri microorganisms	78:123	probiotic Lactobacillus reuteri microorganisms	78:123	Optimisation of oat milk formulation to obtain fermented derivatives by using probiotic Lactobacillus reuteri microorganisms.
24464238	6	1	theme	starters	931:938	arg1	food					1001:1004	a functional food	988:1004	a functional food	988:1004	The selected formulation allowed starters survival above 10(7)/cfu ml to be considered as a functional food and was maintained during the 28 days controlled.
24464238	6	1	theme	starters	931:938	arg1	survival					940:947	starters survival	931:947	starters survival above 10(7)/cfu ml	931:966	The selected formulation allowed starters survival above 10(7)/cfu ml to be considered as a functional food and was maintained during the 28 days controlled.
24464238	1	2	theme	interesting	176:186	arg1	composition					188:198	interesting composition	176:198	interesting composition of oat	176:205	Functional advantages of probiotics combined with interesting composition of oat were considered as an alternative to dairy products.
24464238	7	3	theme	positive	1103:1110	arg1	effect					1112:1117	a positive effect	1101:1117	a positive effect on viscosity	1101:1130	β-glucans remained in the final product with a positive effect on viscosity.
24464238	4	4	theme	acidity	690:696	arg1	colour					739:744	colour	739:744	colour	739:744	Optimised formulation was characterised throughout storage time at 4 ℃ in terms of pH, acidity, β-glucan and oligosaccharides contents, colour and rheological behaviour.
24464238	4	4	theme	acidity	690:696	arg1	behaviour					762:770	rheological behaviour	750:770	rheological behaviour	750:770	Optimised formulation was characterised throughout storage time at 4 ℃ in terms of pH, acidity, β-glucan and oligosaccharides contents, colour and rheological behaviour.
24464238	4	4	theme	acidity	690:696	arg1	contents					729:736	pH, acidity, β-glucan and oligosaccharides contents	686:736	pH, acidity, β-glucan and oligosaccharides contents	686:736	Optimised formulation was characterised throughout storage time at 4 ℃ in terms of pH, acidity, β-glucan and oligosaccharides contents, colour and rheological behaviour.
24464238	8	5	theme	probiotic	1216:1224	arg1	survivals					1226:1234	high probiotic survivals	1211:1234	high probiotic survivals	1211:1234	Therefore, a new probiotic non-dairy milk was successfully developed in which high probiotic survivals were assured throughout the typical yoghurt-like shelf life.
24464238	3	6	with	design	425:430	arg1	methodology					454:464	response surface methodology	437:464	response surface methodology	437:464	Central composite design with response surface methodology was used to analyse the effect of different factors (glucose, fructose, inulin and starters) on the probiotic population in the product.
24464238	4	7	theme	Optimised	603:611	arg1	formulation					613:623	Optimised formulation	603:623	Optimised formulation	603:623	Optimised formulation was characterised throughout storage time at 4 ℃ in terms of pH, acidity, β-glucan and oligosaccharides contents, colour and rheological behaviour.
24464238	0	8	theme	Lactobacillus	88:100	arg1	microorganisms					110:123	probiotic Lactobacillus reuteri microorganisms	78:123	probiotic Lactobacillus reuteri microorganisms	78:123	Optimisation of oat milk formulation to obtain fermented derivatives by using probiotic Lactobacillus reuteri microorganisms.
24464238	6	9	theme	selected	902:909	arg1	formulation					911:921	The selected formulation	898:921	The selected formulation	898:921	The selected formulation allowed starters survival above 10(7)/cfu ml to be considered as a functional food and was maintained during the 28 days controlled.
24464238	3	10	dep	factors	510:516	arg1	factors					510:516	different factors	500:516	different factors (glucose, fructose, inulin and starters)	500:557	Central composite design with response surface methodology was used to analyse the effect of different factors (glucose, fructose, inulin and starters) on the probiotic population in the product.
24464238	3	10	dep	factors	510:516	arg1	fructose					528:535	fructose	528:535	fructose	528:535	Central composite design with response surface methodology was used to analyse the effect of different factors (glucose, fructose, inulin and starters) on the probiotic population in the product.
24464238	3	10	dep	factors	510:516	arg1	glucose					519:525	glucose	519:525	glucose	519:525	Central composite design with response surface methodology was used to analyse the effect of different factors (glucose, fructose, inulin and starters) on the probiotic population in the product.
24464238	3	10	dep	factors	510:516	arg1	starters					549:556	starters	549:556	starters	549:556	Central composite design with response surface methodology was used to analyse the effect of different factors (glucose, fructose, inulin and starters) on the probiotic population in the product.
24464238	3	10	dep	factors	510:516	arg1	inulin					538:543	inulin	538:543	inulin	538:543	Central composite design with response surface methodology was used to analyse the effect of different factors (glucose, fructose, inulin and starters) on the probiotic population in the product.
24464238	2	11	theme	milk	295:298	arg1	fermentation					275:286	fermentation	275:286	fermentation of oat milk with Lactobacillus reuteri and Streptococcus thermophilus	275:356	In this study, fermentation of oat milk with Lactobacillus reuteri and Streptococcus thermophilus was analysed to develop a new probiotic product.
24464238	3	12	theme	factors	510:516	arg1	effect					490:495	the effect	486:495	the effect of different factors (glucose, fructose, inulin and starters) on the probiotic population in the product	486:600	Central composite design with response surface methodology was used to analyse the effect of different factors (glucose, fructose, inulin and starters) on the probiotic population in the product.
24464238	8	13	theme	new	1146:1148	arg1	milk					1170:1173	a new probiotic non-dairy milk	1144:1173	a new probiotic non-dairy milk	1144:1173	Therefore, a new probiotic non-dairy milk was successfully developed in which high probiotic survivals were assured throughout the typical yoghurt-like shelf life.
24464238	8	14	theme	shelf	1285:1289	arg1	life					1291:1294	the typical yoghurt-like shelf life	1260:1294	the typical yoghurt-like shelf life	1260:1294	Therefore, a new probiotic non-dairy milk was successfully developed in which high probiotic survivals were assured throughout the typical yoghurt-like shelf life.
24464238	2	15	theme	oat	291:293	arg1	milk					295:298	oat milk	291:298	oat milk	291:298	In this study, fermentation of oat milk with Lactobacillus reuteri and Streptococcus thermophilus was analysed to develop a new probiotic product.
24464238	5	16	theme	factor	864:869	arg1	optimum					889:895	optimum	889:895	optimum	889:895	All formulations studied were adequate to produce fermented foods and minimum dose of each factor was considered as optimum.
24464238	5	16	theme	factor	864:869	arg1	dose					851:854	minimum dose	843:854	minimum dose of each factor	843:869	All formulations studied were adequate to produce fermented foods and minimum dose of each factor was considered as optimum.
24464238	7	17	theme	final	1082:1086	arg1	product					1088:1094	the final product	1078:1094	the final product	1078:1094	β-glucans remained in the final product with a positive effect on viscosity.
24464238	0	18	dep	Lactobacillus	88:100	arg1	reuteri					102:108	reuteri	102:108	reuteri	102:108	Optimisation of oat milk formulation to obtain fermented derivatives by using probiotic Lactobacillus reuteri microorganisms.
24464238	1	19	theme	oat	203:205	arg1	composition					188:198	interesting composition	176:198	interesting composition of oat	176:205	Functional advantages of probiotics combined with interesting composition of oat were considered as an alternative to dairy products.
24464238	3	20	theme	Central	407:413	arg1	design					425:430	Central composite design	407:430	Central composite design with response surface methodology	407:464	Central composite design with response surface methodology was used to analyse the effect of different factors (glucose, fructose, inulin and starters) on the probiotic population in the product.
24464238	4	21	from	℃	672:672	arg1	terms					677:681	terms	677:681	terms of pH, acidity, β-glucan and oligosaccharides contents, colour and rheological behaviour	677:770	Optimised formulation was characterised throughout storage time at 4 ℃ in terms of pH, acidity, β-glucan and oligosaccharides contents, colour and rheological behaviour.
24464238	3	22	theme	composite	415:423	arg1	design					425:430	Central composite design	407:430	Central composite design with response surface methodology	407:464	Central composite design with response surface methodology was used to analyse the effect of different factors (glucose, fructose, inulin and starters) on the probiotic population in the product.
24464238	4	23	theme	oligosaccharides	712:727	arg1	colour					739:744	colour	739:744	colour	739:744	Optimised formulation was characterised throughout storage time at 4 ℃ in terms of pH, acidity, β-glucan and oligosaccharides contents, colour and rheological behaviour.
24464238	4	23	theme	oligosaccharides	712:727	arg1	behaviour					762:770	rheological behaviour	750:770	rheological behaviour	750:770	Optimised formulation was characterised throughout storage time at 4 ℃ in terms of pH, acidity, β-glucan and oligosaccharides contents, colour and rheological behaviour.
24464238	4	23	theme	oligosaccharides	712:727	arg1	contents					729:736	pH, acidity, β-glucan and oligosaccharides contents	686:736	pH, acidity, β-glucan and oligosaccharides contents	686:736	Optimised formulation was characterised throughout storage time at 4 ℃ in terms of pH, acidity, β-glucan and oligosaccharides contents, colour and rheological behaviour.
24464238	0	24	theme	milk	20:23	arg1	formulation					25:35	oat milk formulation	16:35	oat milk formulation	16:35	Optimisation of oat milk formulation to obtain fermented derivatives by using probiotic Lactobacillus reuteri microorganisms.
24464238	5	25	theme	fermented	823:831	arg1	foods					833:837	fermented foods	823:837	fermented foods	823:837	All formulations studied were adequate to produce fermented foods and minimum dose of each factor was considered as optimum.
24464238	4	26	theme	rheological	750:760	arg1	behaviour					762:770	rheological behaviour	750:770	rheological behaviour	750:770	Optimised formulation was characterised throughout storage time at 4 ℃ in terms of pH, acidity, β-glucan and oligosaccharides contents, colour and rheological behaviour.
24464238	4	26	theme	rheological	750:760	arg1	contents					729:736	pH, acidity, β-glucan and oligosaccharides contents	686:736	pH, acidity, β-glucan and oligosaccharides contents	686:736	Optimised formulation was characterised throughout storage time at 4 ℃ in terms of pH, acidity, β-glucan and oligosaccharides contents, colour and rheological behaviour.
24464238	5	27	theme	minimum	843:849	arg1	optimum					889:895	optimum	889:895	optimum	889:895	All formulations studied were adequate to produce fermented foods and minimum dose of each factor was considered as optimum.
24464238	5	27	theme	minimum	843:849	arg1	dose					851:854	minimum dose	843:854	minimum dose of each factor	843:869	All formulations studied were adequate to produce fermented foods and minimum dose of each factor was considered as optimum.
24464238	0	28	theme	oat	16:18	arg1	formulation					25:35	oat milk formulation	16:35	oat milk formulation	16:35	Optimisation of oat milk formulation to obtain fermented derivatives by using probiotic Lactobacillus reuteri microorganisms.
24464238	6	29	theme	/cfu	960:963	arg1	ml					965:966	10(7)/cfu ml	955:966	10(7)/cfu ml	955:966	The selected formulation allowed starters survival above 10(7)/cfu ml to be considered as a functional food and was maintained during the 28 days controlled.
24464238	3	30	theme	probiotic	566:574	arg1	population					576:585	the probiotic population	562:585	the probiotic population in the product	562:600	Central composite design with response surface methodology was used to analyse the effect of different factors (glucose, fructose, inulin and starters) on the probiotic population in the product.
24464238	4	31	theme	storage	654:660	arg1	time					662:665	storage time	654:665	storage time	654:665	Optimised formulation was characterised throughout storage time at 4 ℃ in terms of pH, acidity, β-glucan and oligosaccharides contents, colour and rheological behaviour.
24464238	3	32	theme	response	437:444	arg1	methodology					454:464	response surface methodology	437:464	response surface methodology	437:464	Central composite design with response surface methodology was used to analyse the effect of different factors (glucose, fructose, inulin and starters) on the probiotic population in the product.
24464238	8	33	theme	yoghurt-like	1272:1283	arg1	life					1291:1294	the typical yoghurt-like shelf life	1260:1294	the typical yoghurt-like shelf life	1260:1294	Therefore, a new probiotic non-dairy milk was successfully developed in which high probiotic survivals were assured throughout the typical yoghurt-like shelf life.
24464238	8	34	theme	typical	1264:1270	arg1	life					1291:1294	the typical yoghurt-like shelf life	1260:1294	the typical yoghurt-like shelf life	1260:1294	Therefore, a new probiotic non-dairy milk was successfully developed in which high probiotic survivals were assured throughout the typical yoghurt-like shelf life.
24464238	3	35	theme	surface	446:452	arg1	methodology					454:464	response surface methodology	437:464	response surface methodology	437:464	Central composite design with response surface methodology was used to analyse the effect of different factors (glucose, fructose, inulin and starters) on the probiotic population in the product.
24464238	0	36	theme	formulation	25:35	arg1	Optimisation					0:11	Optimisation	0:11	Optimisation of oat milk formulation	0:35	Optimisation of oat milk formulation to obtain fermented derivatives by using probiotic Lactobacillus reuteri microorganisms.
24464238	8	37	theme	non-dairy	1160:1168	arg1	milk					1170:1173	a new probiotic non-dairy milk	1144:1173	a new probiotic non-dairy milk	1144:1173	Therefore, a new probiotic non-dairy milk was successfully developed in which high probiotic survivals were assured throughout the typical yoghurt-like shelf life.
24464238	0	38	theme	fermented	47:55	arg1	derivatives					57:67	fermented derivatives	47:67	fermented derivatives	47:67	Optimisation of oat milk formulation to obtain fermented derivatives by using probiotic Lactobacillus reuteri microorganisms.
24464238	1	39	theme	Functional	126:135	arg1	alternative					229:239	an alternative	226:239	an alternative to dairy products	226:257	Functional advantages of probiotics combined with interesting composition of oat were considered as an alternative to dairy products.
24464238	1	39	theme	Functional	126:135	arg1	advantages					137:146	Functional advantages	126:146	Functional advantages of probiotics combined with interesting composition of oat	126:205	Functional advantages of probiotics combined with interesting composition of oat were considered as an alternative to dairy products.
24464238	2	40	with	fermentation	275:286	arg1	reuteri					319:325	Lactobacillus reuteri	305:325	Lactobacillus reuteri	305:325	In this study, fermentation of oat milk with Lactobacillus reuteri and Streptococcus thermophilus was analysed to develop a new probiotic product.
24464238	2	40	with	fermentation	275:286	arg1	thermophilus					345:356	Streptococcus thermophilus	331:356	Streptococcus thermophilus	331:356	In this study, fermentation of oat milk with Lactobacillus reuteri and Streptococcus thermophilus was analysed to develop a new probiotic product.
24464238	3	41	from	effect	490:495	arg1	population					576:585	the probiotic population	562:585	the probiotic population in the product	562:600	Central composite design with response surface methodology was used to analyse the effect of different factors (glucose, fructose, inulin and starters) on the probiotic population in the product.
24464238	4	42	theme	β-glucan	699:706	arg1	colour					739:744	colour	739:744	colour	739:744	Optimised formulation was characterised throughout storage time at 4 ℃ in terms of pH, acidity, β-glucan and oligosaccharides contents, colour and rheological behaviour.
24464238	4	42	theme	β-glucan	699:706	arg1	behaviour					762:770	rheological behaviour	750:770	rheological behaviour	750:770	Optimised formulation was characterised throughout storage time at 4 ℃ in terms of pH, acidity, β-glucan and oligosaccharides contents, colour and rheological behaviour.
24464238	4	42	theme	β-glucan	699:706	arg1	contents					729:736	pH, acidity, β-glucan and oligosaccharides contents	686:736	pH, acidity, β-glucan and oligosaccharides contents	686:736	Optimised formulation was characterised throughout storage time at 4 ℃ in terms of pH, acidity, β-glucan and oligosaccharides contents, colour and rheological behaviour.
24464238	4	43	theme	pH	686:687	arg1	colour					739:744	colour	739:744	colour	739:744	Optimised formulation was characterised throughout storage time at 4 ℃ in terms of pH, acidity, β-glucan and oligosaccharides contents, colour and rheological behaviour.
24464238	4	43	theme	pH	686:687	arg1	behaviour					762:770	rheological behaviour	750:770	rheological behaviour	750:770	Optimised formulation was characterised throughout storage time at 4 ℃ in terms of pH, acidity, β-glucan and oligosaccharides contents, colour and rheological behaviour.
24464238	4	43	theme	pH	686:687	arg1	contents					729:736	pH, acidity, β-glucan and oligosaccharides contents	686:736	pH, acidity, β-glucan and oligosaccharides contents	686:736	Optimised formulation was characterised throughout storage time at 4 ℃ in terms of pH, acidity, β-glucan and oligosaccharides contents, colour and rheological behaviour.
24464238	3	44	theme	different	500:508	arg1	fructose					528:535	fructose	528:535	fructose	528:535	Central composite design with response surface methodology was used to analyse the effect of different factors (glucose, fructose, inulin and starters) on the probiotic population in the product.
24464238	3	44	theme	different	500:508	arg1	glucose					519:525	glucose	519:525	glucose	519:525	Central composite design with response surface methodology was used to analyse the effect of different factors (glucose, fructose, inulin and starters) on the probiotic population in the product.
24464238	3	44	theme	different	500:508	arg1	factors					510:516	different factors	500:516	different factors (glucose, fructose, inulin and starters)	500:557	Central composite design with response surface methodology was used to analyse the effect of different factors (glucose, fructose, inulin and starters) on the probiotic population in the product.
24464238	3	44	theme	different	500:508	arg1	inulin					538:543	inulin	538:543	inulin	538:543	Central composite design with response surface methodology was used to analyse the effect of different factors (glucose, fructose, inulin and starters) on the probiotic population in the product.
24464238	1	45	theme	dairy	244:248	arg1	products					250:257	dairy products	244:257	dairy products	244:257	Functional advantages of probiotics combined with interesting composition of oat were considered as an alternative to dairy products.
24464238	3	46	used	used	470:473	arg2	design					425:430	Central composite design	407:430	Central composite design with response surface methodology	407:464	Central composite design with response surface methodology was used to analyse the effect of different factors (glucose, fructose, inulin and starters) on the probiotic population in the product.
24464238	8	47	theme	probiotic	1150:1158	arg1	milk					1170:1173	a new probiotic non-dairy milk	1144:1173	a new probiotic non-dairy milk	1144:1173	Therefore, a new probiotic non-dairy milk was successfully developed in which high probiotic survivals were assured throughout the typical yoghurt-like shelf life.
24464238	1	48	theme	probiotics	151:160	arg1	alternative					229:239	an alternative	226:239	an alternative to dairy products	226:257	Functional advantages of probiotics combined with interesting composition of oat were considered as an alternative to dairy products.
24464238	1	48	theme	probiotics	151:160	arg1	advantages					137:146	Functional advantages	126:146	Functional advantages of probiotics combined with interesting composition of oat	126:205	Functional advantages of probiotics combined with interesting composition of oat were considered as an alternative to dairy products.
24464238	3	49	from	population	576:585	arg1	product					594:600	the product	590:600	the product	590:600	Central composite design with response surface methodology was used to analyse the effect of different factors (glucose, fructose, inulin and starters) on the probiotic population in the product.
24464238	4	50	theme	contents	729:736	arg1	terms					677:681	terms	677:681	terms of pH, acidity, β-glucan and oligosaccharides contents, colour and rheological behaviour	677:770	Optimised formulation was characterised throughout storage time at 4 ℃ in terms of pH, acidity, β-glucan and oligosaccharides contents, colour and rheological behaviour.
24464238	2	51	theme	probiotic	388:396	arg1	product					398:404	a new probiotic product	382:404	a new probiotic product	382:404	In this study, fermentation of oat milk with Lactobacillus reuteri and Streptococcus thermophilus was analysed to develop a new probiotic product.
24464238	8	52	theme	high	1211:1214	arg1	survivals					1226:1234	high probiotic survivals	1211:1234	high probiotic survivals	1211:1234	Therefore, a new probiotic non-dairy milk was successfully developed in which high probiotic survivals were assured throughout the typical yoghurt-like shelf life.
24464238	7	53	from	effect	1112:1117	arg1	viscosity					1122:1130	viscosity	1122:1130	viscosity	1122:1130	β-glucans remained in the final product with a positive effect on viscosity.
24464238	2	54	theme	new	384:386	arg1	product					398:404	a new probiotic product	382:404	a new probiotic product	382:404	In this study, fermentation of oat milk with Lactobacillus reuteri and Streptococcus thermophilus was analysed to develop a new probiotic product.
24464238	6	55	theme	functional	990:999	arg1	food					1001:1004	a functional food	988:1004	a functional food	988:1004	The selected formulation allowed starters survival above 10(7)/cfu ml to be considered as a functional food and was maintained during the 28 days controlled.
24464238	6	55	theme	functional	990:999	arg1	survival					940:947	starters survival	931:947	starters survival above 10(7)/cfu ml	931:966	The selected formulation allowed starters survival above 10(7)/cfu ml to be considered as a functional food and was maintained during the 28 days controlled.
27352306	0	0	theme	[Syzygium	95:103	arg1	Merr					121:124	Malay Apple [Syzygium malaccense (L.) Merr	83:124	Malay Apple [Syzygium malaccense (L.) Merr	83:124	Physico-Chemical Characterization, Bioactive Compounds and Antioxidant Activity of Malay Apple [Syzygium malaccense (L.) Merr.
27352306	7	1	theme	Malay	1161:1165	arg1	fruit					1173:1177	Malay apple fruit	1161:1177	Malay apple fruit	1161:1177	This study highlights the potential of Malay apple fruit as a good source of antioxidant compounds with potential benefits to human health.
27352306	4	2	theme	anthocyanins	648:659	arg1	contents					677:684	Total phenolics (1293 mg gallic acid equivalent/100 g) and total anthocyanins (1045 mg/100 g) contents	583:684	Total phenolics (1293 mg gallic acid equivalent/100 g) and total anthocyanins (1045 mg/100 g) contents	583:684	Total phenolics (1293 mg gallic acid equivalent/100 g) and total anthocyanins (1045 mg/100 g) contents were higher in the peel, with the major anthocyanin identified using HPLC-DAD-MS/MS as cyanidin 3-glucoside.
27352306	0	3	theme	Apple	89:93	arg1	Merr					121:124	Malay Apple [Syzygium malaccense (L.) Merr	83:124	Malay Apple [Syzygium malaccense (L.) Merr	83:124	Physico-Chemical Characterization, Bioactive Compounds and Antioxidant Activity of Malay Apple [Syzygium malaccense (L.) Merr.
27352306	4	4	theme	total	642:646	arg1	anthocyanins					648:659	total anthocyanins	642:659	total anthocyanins (1045 mg/100 g)	642:675	Total phenolics (1293 mg gallic acid equivalent/100 g) and total anthocyanins (1045 mg/100 g) contents were higher in the peel, with the major anthocyanin identified using HPLC-DAD-MS/MS as cyanidin 3-glucoside.
27352306	4	4	theme	total	642:646	arg1	g					674:674	1045 mg/100 g	662:674	1045 mg/100 g	662:674	Total phenolics (1293 mg gallic acid equivalent/100 g) and total anthocyanins (1045 mg/100 g) contents were higher in the peel, with the major anthocyanin identified using HPLC-DAD-MS/MS as cyanidin 3-glucoside.
27352306	5	5	theme	peel	1007:1010	arg1	fraction					1012:1019	the peel fraction	1003:1019	the peel fraction	1003:1019	Higher values for DPPH antiradical scavenging activity (47.52 μMol trolox equivalent antioxidant capacity/g) and Ferric Reducing Antioxidant Potential (FRAP, 0.19 mM ferreous sulfate/g) were also observed in the peel fraction.
27352306	5	6	theme	ferreous	961:968	arg1	sulfate/g					970:978	0.19 mM ferreous sulfate/g	953:978	0.19 mM ferreous sulfate/g	953:978	Higher values for DPPH antiradical scavenging activity (47.52 μMol trolox equivalent antioxidant capacity/g) and Ferric Reducing Antioxidant Potential (FRAP, 0.19 mM ferreous sulfate/g) were also observed in the peel fraction.
27352306	5	6	theme	ferreous	961:968	arg1	FRAP					947:950	FRAP	947:950	FRAP	947:950	Higher values for DPPH antiradical scavenging activity (47.52 μMol trolox equivalent antioxidant capacity/g) and Ferric Reducing Antioxidant Potential (FRAP, 0.19 mM ferreous sulfate/g) were also observed in the peel fraction.
27352306	3	7	theme	ascorbic	490:497	arg1	acid					499:502	ascorbic acid	490:502	ascorbic acid	490:502	Fruit diameter, weight, yield, and centesimal composition, ascorbic acid, reductive sugars, total soluble solids, pH and fiber content were determined.
27352306	2	8	theme	fruit	282:286	arg1	characteristics					205:219	the physico-chemical characteristics	184:219	the physico-chemical characteristics	184:219	The purpose of this study was to evaluate the physico-chemical characteristics, bioactive compounds and antioxidant activity of Malay apple fruit (Syzygium malaccense) grown in Brazil with regard to the geographical origin and its peel fractions and edible portion analyzed independently.
27352306	2	8	theme	fruit	282:286	arg1	compounds					232:240	bioactive compounds	222:240	bioactive compounds	222:240	The purpose of this study was to evaluate the physico-chemical characteristics, bioactive compounds and antioxidant activity of Malay apple fruit (Syzygium malaccense) grown in Brazil with regard to the geographical origin and its peel fractions and edible portion analyzed independently.
27352306	2	8	theme	fruit	282:286	arg1	activity					258:265	antioxidant activity	246:265	antioxidant activity	246:265	The purpose of this study was to evaluate the physico-chemical characteristics, bioactive compounds and antioxidant activity of Malay apple fruit (Syzygium malaccense) grown in Brazil with regard to the geographical origin and its peel fractions and edible portion analyzed independently.
27352306	5	9	theme	Ferric	908:913	arg1	Potential					936:944	Ferric Reducing Antioxidant Potential	908:944	Ferric Reducing Antioxidant Potential (FRAP, 0.19 mM ferreous sulfate/g)	908:979	Higher values for DPPH antiradical scavenging activity (47.52 μMol trolox equivalent antioxidant capacity/g) and Ferric Reducing Antioxidant Potential (FRAP, 0.19 mM ferreous sulfate/g) were also observed in the peel fraction.
27352306	5	10	theme	Reducing	915:922	arg1	Potential					936:944	Ferric Reducing Antioxidant Potential	908:944	Ferric Reducing Antioxidant Potential (FRAP, 0.19 mM ferreous sulfate/g)	908:979	Higher values for DPPH antiradical scavenging activity (47.52 μMol trolox equivalent antioxidant capacity/g) and Ferric Reducing Antioxidant Potential (FRAP, 0.19 mM ferreous sulfate/g) were also observed in the peel fraction.
27352306	0	11	theme	malaccense	105:114	arg1	Merr					121:124	Malay Apple [Syzygium malaccense (L.) Merr	83:124	Malay Apple [Syzygium malaccense (L.) Merr	83:124	Physico-Chemical Characterization, Bioactive Compounds and Antioxidant Activity of Malay Apple [Syzygium malaccense (L.) Merr.
27352306	5	12	theme	Antioxidant	924:934	arg1	Potential					936:944	Ferric Reducing Antioxidant Potential	908:944	Ferric Reducing Antioxidant Potential (FRAP, 0.19 mM ferreous sulfate/g)	908:979	Higher values for DPPH antiradical scavenging activity (47.52 μMol trolox equivalent antioxidant capacity/g) and Ferric Reducing Antioxidant Potential (FRAP, 0.19 mM ferreous sulfate/g) were also observed in the peel fraction.
27352306	7	13	theme	good	1184:1187	arg1	source					1189:1194	a good source	1182:1194	a good source of antioxidant compounds with potential benefits to human health	1182:1259	This study highlights the potential of Malay apple fruit as a good source of antioxidant compounds with potential benefits to human health.
27352306	7	13	theme	good	1184:1187	arg1	potential					1148:1156	the potential	1144:1156	the potential of Malay apple fruit	1144:1177	This study highlights the potential of Malay apple fruit as a good source of antioxidant compounds with potential benefits to human health.
27352306	2	14	theme	apple	276:280	arg1	fruit					282:286	Malay apple fruit	270:286	Malay apple fruit (Syzygium malaccense) grown in Brazil with regard to the geographical origin and its peel fractions and edible portion analyzed independently	270:428	The purpose of this study was to evaluate the physico-chemical characteristics, bioactive compounds and antioxidant activity of Malay apple fruit (Syzygium malaccense) grown in Brazil with regard to the geographical origin and its peel fractions and edible portion analyzed independently.
27352306	2	14	theme	apple	276:280	arg1	malaccense					298:307	Syzygium malaccense	289:307	Syzygium malaccense	289:307	The purpose of this study was to evaluate the physico-chemical characteristics, bioactive compounds and antioxidant activity of Malay apple fruit (Syzygium malaccense) grown in Brazil with regard to the geographical origin and its peel fractions and edible portion analyzed independently.
27352306	5	15	theme	antioxidant	880:890	arg1	capacity/g					892:901	47.52 μMol trolox equivalent antioxidant capacity/g	851:901	47.52 μMol trolox equivalent antioxidant capacity/g	851:901	Higher values for DPPH antiradical scavenging activity (47.52 μMol trolox equivalent antioxidant capacity/g) and Ferric Reducing Antioxidant Potential (FRAP, 0.19 mM ferreous sulfate/g) were also observed in the peel fraction.
27352306	5	15	theme	antioxidant	880:890	arg1	activity					841:848	DPPH antiradical scavenging activity	813:848	DPPH antiradical scavenging activity (47.52 μMol trolox equivalent antioxidant capacity/g)	813:902	Higher values for DPPH antiradical scavenging activity (47.52 μMol trolox equivalent antioxidant capacity/g) and Ferric Reducing Antioxidant Potential (FRAP, 0.19 mM ferreous sulfate/g) were also observed in the peel fraction.
27352306	5	16	dep	Potential	936:944	arg1	sulfate/g					970:978	0.19 mM ferreous sulfate/g	953:978	0.19 mM ferreous sulfate/g	953:978	Higher values for DPPH antiradical scavenging activity (47.52 μMol trolox equivalent antioxidant capacity/g) and Ferric Reducing Antioxidant Potential (FRAP, 0.19 mM ferreous sulfate/g) were also observed in the peel fraction.
27352306	5	16	dep	Potential	936:944	arg1	FRAP					947:950	FRAP	947:950	FRAP	947:950	Higher values for DPPH antiradical scavenging activity (47.52 μMol trolox equivalent antioxidant capacity/g) and Ferric Reducing Antioxidant Potential (FRAP, 0.19 mM ferreous sulfate/g) were also observed in the peel fraction.
27352306	4	17	theme	phenolics	589:597	arg1	contents					677:684	Total phenolics (1293 mg gallic acid equivalent/100 g) and total anthocyanins (1045 mg/100 g) contents	583:684	Total phenolics (1293 mg gallic acid equivalent/100 g) and total anthocyanins (1045 mg/100 g) contents	583:684	Total phenolics (1293 mg gallic acid equivalent/100 g) and total anthocyanins (1045 mg/100 g) contents were higher in the peel, with the major anthocyanin identified using HPLC-DAD-MS/MS as cyanidin 3-glucoside.
27352306	2	18	theme	Malay	270:274	arg1	fruit					282:286	Malay apple fruit	270:286	Malay apple fruit (Syzygium malaccense) grown in Brazil with regard to the geographical origin and its peel fractions and edible portion analyzed independently	270:428	The purpose of this study was to evaluate the physico-chemical characteristics, bioactive compounds and antioxidant activity of Malay apple fruit (Syzygium malaccense) grown in Brazil with regard to the geographical origin and its peel fractions and edible portion analyzed independently.
27352306	2	18	theme	Malay	270:274	arg1	malaccense					298:307	Syzygium malaccense	289:307	Syzygium malaccense	289:307	The purpose of this study was to evaluate the physico-chemical characteristics, bioactive compounds and antioxidant activity of Malay apple fruit (Syzygium malaccense) grown in Brazil with regard to the geographical origin and its peel fractions and edible portion analyzed independently.
27352306	2	19	theme	peel	373:376	arg1	fractions					378:386	its peel fractions	369:386	its peel fractions	369:386	The purpose of this study was to evaluate the physico-chemical characteristics, bioactive compounds and antioxidant activity of Malay apple fruit (Syzygium malaccense) grown in Brazil with regard to the geographical origin and its peel fractions and edible portion analyzed independently.
27352306	0	20	theme	L.	117:118	arg1	Merr					121:124	Malay Apple [Syzygium malaccense (L.) Merr	83:124	Malay Apple [Syzygium malaccense (L.) Merr	83:124	Physico-Chemical Characterization, Bioactive Compounds and Antioxidant Activity of Malay Apple [Syzygium malaccense (L.) Merr.
27352306	4	21	theme	cyanidin	773:780	arg1	3-glucoside					782:792	cyanidin 3-glucoside	773:792	cyanidin 3-glucoside	773:792	Total phenolics (1293 mg gallic acid equivalent/100 g) and total anthocyanins (1045 mg/100 g) contents were higher in the peel, with the major anthocyanin identified using HPLC-DAD-MS/MS as cyanidin 3-glucoside.
27352306	3	22	theme	reductive	505:513	arg1	sugars					515:520	reductive sugars	505:520	reductive sugars	505:520	Fruit diameter, weight, yield, and centesimal composition, ascorbic acid, reductive sugars, total soluble solids, pH and fiber content were determined.
27352306	3	23	theme	Fruit	431:435	arg1	diameter					437:444	Fruit diameter	431:444	Fruit diameter	431:444	Fruit diameter, weight, yield, and centesimal composition, ascorbic acid, reductive sugars, total soluble solids, pH and fiber content were determined.
27352306	7	24	theme	apple	1167:1171	arg1	fruit					1173:1177	Malay apple fruit	1161:1177	Malay apple fruit	1161:1177	This study highlights the potential of Malay apple fruit as a good source of antioxidant compounds with potential benefits to human health.
27352306	7	25	theme	fruit	1173:1177	arg1	potential					1148:1156	the potential	1144:1156	the potential of Malay apple fruit	1144:1177	This study highlights the potential of Malay apple fruit as a good source of antioxidant compounds with potential benefits to human health.
27352306	7	25	theme	fruit	1173:1177	arg1	source					1189:1194	a good source	1182:1194	a good source of antioxidant compounds with potential benefits to human health	1182:1259	This study highlights the potential of Malay apple fruit as a good source of antioxidant compounds with potential benefits to human health.
27352306	6	26	theme	acid	1109:1112	arg1	system					1114:1119	the β-carotene/linoleic acid system	1085:1119	the β-carotene/linoleic acid system	1085:1119	All extracts tested showed the ability to inhibit oxidation in the β-carotene/linoleic acid system.
27352306	7	27	theme	compounds	1211:1219	arg1	source					1189:1194	a good source	1182:1194	a good source of antioxidant compounds with potential benefits to human health	1182:1259	This study highlights the potential of Malay apple fruit as a good source of antioxidant compounds with potential benefits to human health.
27352306	7	27	theme	compounds	1211:1219	arg1	potential					1148:1156	the potential	1144:1156	the potential of Malay apple fruit	1144:1177	This study highlights the potential of Malay apple fruit as a good source of antioxidant compounds with potential benefits to human health.
27352306	5	28	theme	DPPH	813:816	arg1	capacity/g					892:901	47.52 μMol trolox equivalent antioxidant capacity/g	851:901	47.52 μMol trolox equivalent antioxidant capacity/g	851:901	Higher values for DPPH antiradical scavenging activity (47.52 μMol trolox equivalent antioxidant capacity/g) and Ferric Reducing Antioxidant Potential (FRAP, 0.19 mM ferreous sulfate/g) were also observed in the peel fraction.
27352306	5	28	theme	DPPH	813:816	arg1	activity					841:848	DPPH antiradical scavenging activity	813:848	DPPH antiradical scavenging activity (47.52 μMol trolox equivalent antioxidant capacity/g)	813:902	Higher values for DPPH antiradical scavenging activity (47.52 μMol trolox equivalent antioxidant capacity/g) and Ferric Reducing Antioxidant Potential (FRAP, 0.19 mM ferreous sulfate/g) were also observed in the peel fraction.
27352306	0	29	theme	Physico-Chemical	0:15	arg1	Characterization					17:32	Physico-Chemical Characterization	0:32	Physico-Chemical Characterization	0:32	Physico-Chemical Characterization, Bioactive Compounds and Antioxidant Activity of Malay Apple [Syzygium malaccense (L.) Merr.
27352306	6	30	theme	β-carotene/linoleic	1089:1107	arg1	system					1114:1119	the β-carotene/linoleic acid system	1085:1119	the β-carotene/linoleic acid system	1085:1119	All extracts tested showed the ability to inhibit oxidation in the β-carotene/linoleic acid system.
27352306	5	31	theme	antiradical	818:828	arg1	capacity/g					892:901	47.52 μMol trolox equivalent antioxidant capacity/g	851:901	47.52 μMol trolox equivalent antioxidant capacity/g	851:901	Higher values for DPPH antiradical scavenging activity (47.52 μMol trolox equivalent antioxidant capacity/g) and Ferric Reducing Antioxidant Potential (FRAP, 0.19 mM ferreous sulfate/g) were also observed in the peel fraction.
27352306	5	31	theme	antiradical	818:828	arg1	activity					841:848	DPPH antiradical scavenging activity	813:848	DPPH antiradical scavenging activity (47.52 μMol trolox equivalent antioxidant capacity/g)	813:902	Higher values for DPPH antiradical scavenging activity (47.52 μMol trolox equivalent antioxidant capacity/g) and Ferric Reducing Antioxidant Potential (FRAP, 0.19 mM ferreous sulfate/g) were also observed in the peel fraction.
27352306	0	32	theme	Bioactive	35:43	arg1	Compounds					45:53	Bioactive Compounds	35:53	Bioactive Compounds	35:53	Physico-Chemical Characterization, Bioactive Compounds and Antioxidant Activity of Malay Apple [Syzygium malaccense (L.) Merr.
27352306	5	33	theme	0.19	953:956	arg1	mM					958:959	mM	958:959	mM	958:959	Higher values for DPPH antiradical scavenging activity (47.52 μMol trolox equivalent antioxidant capacity/g) and Ferric Reducing Antioxidant Potential (FRAP, 0.19 mM ferreous sulfate/g) were also observed in the peel fraction.
27352306	4	34	dep	phenolics	589:597	arg1	acid					615:618	1293 mg gallic acid	600:618	1293 mg gallic acid equivalent/100 g	600:635	Total phenolics (1293 mg gallic acid equivalent/100 g) and total anthocyanins (1045 mg/100 g) contents were higher in the peel, with the major anthocyanin identified using HPLC-DAD-MS/MS as cyanidin 3-glucoside.
27352306	2	35	theme	Syzygium	289:296	arg1	fruit					282:286	Malay apple fruit	270:286	Malay apple fruit (Syzygium malaccense) grown in Brazil with regard to the geographical origin and its peel fractions and edible portion analyzed independently	270:428	The purpose of this study was to evaluate the physico-chemical characteristics, bioactive compounds and antioxidant activity of Malay apple fruit (Syzygium malaccense) grown in Brazil with regard to the geographical origin and its peel fractions and edible portion analyzed independently.
27352306	2	35	theme	Syzygium	289:296	arg1	malaccense					298:307	Syzygium malaccense	289:307	Syzygium malaccense	289:307	The purpose of this study was to evaluate the physico-chemical characteristics, bioactive compounds and antioxidant activity of Malay apple fruit (Syzygium malaccense) grown in Brazil with regard to the geographical origin and its peel fractions and edible portion analyzed independently.
27352306	2	36	theme	bioactive	222:230	arg1	compounds					232:240	bioactive compounds	222:240	bioactive compounds	222:240	The purpose of this study was to evaluate the physico-chemical characteristics, bioactive compounds and antioxidant activity of Malay apple fruit (Syzygium malaccense) grown in Brazil with regard to the geographical origin and its peel fractions and edible portion analyzed independently.
27352306	5	37	located	observed	991:998	arg1	fraction					1012:1019	the peel fraction	1003:1019	the peel fraction	1003:1019	Higher values for DPPH antiradical scavenging activity (47.52 μMol trolox equivalent antioxidant capacity/g) and Ferric Reducing Antioxidant Potential (FRAP, 0.19 mM ferreous sulfate/g) were also observed in the peel fraction.
27352306	5	37	located	observed	991:998	arg2	values					802:807	Higher values	795:807	Higher values for DPPH antiradical scavenging activity (47.52 μMol trolox equivalent antioxidant capacity/g) and Ferric Reducing Antioxidant Potential (FRAP, 0.19 mM ferreous sulfate/g)	795:979	Higher values for DPPH antiradical scavenging activity (47.52 μMol trolox equivalent antioxidant capacity/g) and Ferric Reducing Antioxidant Potential (FRAP, 0.19 mM ferreous sulfate/g) were also observed in the peel fraction.
27352306	0	38	theme	Merr	121:124	arg1	Characterization					17:32	Physico-Chemical Characterization	0:32	Physico-Chemical Characterization	0:32	Physico-Chemical Characterization, Bioactive Compounds and Antioxidant Activity of Malay Apple [Syzygium malaccense (L.) Merr.
27352306	0	38	theme	Merr	121:124	arg1	Compounds					45:53	Bioactive Compounds	35:53	Bioactive Compounds	35:53	Physico-Chemical Characterization, Bioactive Compounds and Antioxidant Activity of Malay Apple [Syzygium malaccense (L.) Merr.
27352306	0	38	theme	Merr	121:124	arg1	Activity					71:78	Antioxidant Activity	59:78	Antioxidant Activity	59:78	Physico-Chemical Characterization, Bioactive Compounds and Antioxidant Activity of Malay Apple [Syzygium malaccense (L.) Merr.
27352306	2	39	theme	study	162:166	arg1	purpose					146:152	The purpose	142:152	The purpose of this study	142:166	The purpose of this study was to evaluate the physico-chemical characteristics, bioactive compounds and antioxidant activity of Malay apple fruit (Syzygium malaccense) grown in Brazil with regard to the geographical origin and its peel fractions and edible portion analyzed independently.
27352306	3	40	theme	soluble	529:535	arg1	solids					537:542	total soluble solids	523:542	total soluble solids	523:542	Fruit diameter, weight, yield, and centesimal composition, ascorbic acid, reductive sugars, total soluble solids, pH and fiber content were determined.
27352306	2	41	theme	edible	392:397	arg1	portion					399:405	edible portion	392:405	edible portion	392:405	The purpose of this study was to evaluate the physico-chemical characteristics, bioactive compounds and antioxidant activity of Malay apple fruit (Syzygium malaccense) grown in Brazil with regard to the geographical origin and its peel fractions and edible portion analyzed independently.
27352306	5	42	theme	trolox	862:867	arg1	capacity/g					892:901	47.52 μMol trolox equivalent antioxidant capacity/g	851:901	47.52 μMol trolox equivalent antioxidant capacity/g	851:901	Higher values for DPPH antiradical scavenging activity (47.52 μMol trolox equivalent antioxidant capacity/g) and Ferric Reducing Antioxidant Potential (FRAP, 0.19 mM ferreous sulfate/g) were also observed in the peel fraction.
27352306	5	42	theme	trolox	862:867	arg1	activity					841:848	DPPH antiradical scavenging activity	813:848	DPPH antiradical scavenging activity (47.52 μMol trolox equivalent antioxidant capacity/g)	813:902	Higher values for DPPH antiradical scavenging activity (47.52 μMol trolox equivalent antioxidant capacity/g) and Ferric Reducing Antioxidant Potential (FRAP, 0.19 mM ferreous sulfate/g) were also observed in the peel fraction.
27352306	3	43	theme	fiber	552:556	arg1	content					558:564	fiber content	552:564	fiber content	552:564	Fruit diameter, weight, yield, and centesimal composition, ascorbic acid, reductive sugars, total soluble solids, pH and fiber content were determined.
27352306	4	44	theme	mg	605:606	arg1	acid					615:618	1293 mg gallic acid	600:618	1293 mg gallic acid equivalent/100 g	600:635	Total phenolics (1293 mg gallic acid equivalent/100 g) and total anthocyanins (1045 mg/100 g) contents were higher in the peel, with the major anthocyanin identified using HPLC-DAD-MS/MS as cyanidin 3-glucoside.
27352306	7	45	with	compounds	1211:1219	arg1	benefits					1236:1243	potential benefits	1226:1243	potential benefits to human health	1226:1259	This study highlights the potential of Malay apple fruit as a good source of antioxidant compounds with potential benefits to human health.
27352306	4	46	theme	1293	600:603	arg1	mg					605:606	mg	605:606	mg	605:606	Total phenolics (1293 mg gallic acid equivalent/100 g) and total anthocyanins (1045 mg/100 g) contents were higher in the peel, with the major anthocyanin identified using HPLC-DAD-MS/MS as cyanidin 3-glucoside.
27352306	5	47	theme	scavenging	830:839	arg1	capacity/g					892:901	47.52 μMol trolox equivalent antioxidant capacity/g	851:901	47.52 μMol trolox equivalent antioxidant capacity/g	851:901	Higher values for DPPH antiradical scavenging activity (47.52 μMol trolox equivalent antioxidant capacity/g) and Ferric Reducing Antioxidant Potential (FRAP, 0.19 mM ferreous sulfate/g) were also observed in the peel fraction.
27352306	5	47	theme	scavenging	830:839	arg1	activity					841:848	DPPH antiradical scavenging activity	813:848	DPPH antiradical scavenging activity (47.52 μMol trolox equivalent antioxidant capacity/g)	813:902	Higher values for DPPH antiradical scavenging activity (47.52 μMol trolox equivalent antioxidant capacity/g) and Ferric Reducing Antioxidant Potential (FRAP, 0.19 mM ferreous sulfate/g) were also observed in the peel fraction.
27352306	5	48	theme	μMol	857:860	arg1	capacity/g					892:901	47.52 μMol trolox equivalent antioxidant capacity/g	851:901	47.52 μMol trolox equivalent antioxidant capacity/g	851:901	Higher values for DPPH antiradical scavenging activity (47.52 μMol trolox equivalent antioxidant capacity/g) and Ferric Reducing Antioxidant Potential (FRAP, 0.19 mM ferreous sulfate/g) were also observed in the peel fraction.
27352306	5	48	theme	μMol	857:860	arg1	activity					841:848	DPPH antiradical scavenging activity	813:848	DPPH antiradical scavenging activity (47.52 μMol trolox equivalent antioxidant capacity/g)	813:902	Higher values for DPPH antiradical scavenging activity (47.52 μMol trolox equivalent antioxidant capacity/g) and Ferric Reducing Antioxidant Potential (FRAP, 0.19 mM ferreous sulfate/g) were also observed in the peel fraction.
27352306	0	49	theme	Antioxidant	59:69	arg1	Activity					71:78	Antioxidant Activity	59:78	Antioxidant Activity	59:78	Physico-Chemical Characterization, Bioactive Compounds and Antioxidant Activity of Malay Apple [Syzygium malaccense (L.) Merr.
27352306	2	50	theme	physico-chemical	188:203	arg1	characteristics					205:219	the physico-chemical characteristics	184:219	the physico-chemical characteristics	184:219	The purpose of this study was to evaluate the physico-chemical characteristics, bioactive compounds and antioxidant activity of Malay apple fruit (Syzygium malaccense) grown in Brazil with regard to the geographical origin and its peel fractions and edible portion analyzed independently.
27352306	3	51	theme	centesimal	466:475	arg1	composition					477:487	centesimal composition	466:487	centesimal composition	466:487	Fruit diameter, weight, yield, and centesimal composition, ascorbic acid, reductive sugars, total soluble solids, pH and fiber content were determined.
27352306	5	52	theme	mM	958:959	arg1	sulfate/g					970:978	0.19 mM ferreous sulfate/g	953:978	0.19 mM ferreous sulfate/g	953:978	Higher values for DPPH antiradical scavenging activity (47.52 μMol trolox equivalent antioxidant capacity/g) and Ferric Reducing Antioxidant Potential (FRAP, 0.19 mM ferreous sulfate/g) were also observed in the peel fraction.
27352306	5	52	theme	mM	958:959	arg1	FRAP					947:950	FRAP	947:950	FRAP	947:950	Higher values for DPPH antiradical scavenging activity (47.52 μMol trolox equivalent antioxidant capacity/g) and Ferric Reducing Antioxidant Potential (FRAP, 0.19 mM ferreous sulfate/g) were also observed in the peel fraction.
27352306	5	53	theme	equivalent	869:878	arg1	capacity/g					892:901	47.52 μMol trolox equivalent antioxidant capacity/g	851:901	47.52 μMol trolox equivalent antioxidant capacity/g	851:901	Higher values for DPPH antiradical scavenging activity (47.52 μMol trolox equivalent antioxidant capacity/g) and Ferric Reducing Antioxidant Potential (FRAP, 0.19 mM ferreous sulfate/g) were also observed in the peel fraction.
27352306	5	53	theme	equivalent	869:878	arg1	activity					841:848	DPPH antiradical scavenging activity	813:848	DPPH antiradical scavenging activity (47.52 μMol trolox equivalent antioxidant capacity/g)	813:902	Higher values for DPPH antiradical scavenging activity (47.52 μMol trolox equivalent antioxidant capacity/g) and Ferric Reducing Antioxidant Potential (FRAP, 0.19 mM ferreous sulfate/g) were also observed in the peel fraction.
27352306	3	54	theme	total	523:527	arg1	solids					537:542	total soluble solids	523:542	total soluble solids	523:542	Fruit diameter, weight, yield, and centesimal composition, ascorbic acid, reductive sugars, total soluble solids, pH and fiber content were determined.
27352306	7	55	theme	potential	1226:1234	arg1	benefits					1236:1243	potential benefits	1226:1243	potential benefits to human health	1226:1259	This study highlights the potential of Malay apple fruit as a good source of antioxidant compounds with potential benefits to human health.
27352306	2	56	theme	antioxidant	246:256	arg1	activity					258:265	antioxidant activity	246:265	antioxidant activity	246:265	The purpose of this study was to evaluate the physico-chemical characteristics, bioactive compounds and antioxidant activity of Malay apple fruit (Syzygium malaccense) grown in Brazil with regard to the geographical origin and its peel fractions and edible portion analyzed independently.
27352306	4	57	theme	mg/100	667:672	arg1	anthocyanins					648:659	total anthocyanins	642:659	total anthocyanins (1045 mg/100 g)	642:675	Total phenolics (1293 mg gallic acid equivalent/100 g) and total anthocyanins (1045 mg/100 g) contents were higher in the peel, with the major anthocyanin identified using HPLC-DAD-MS/MS as cyanidin 3-glucoside.
27352306	4	57	theme	mg/100	667:672	arg1	g					674:674	1045 mg/100 g	662:674	1045 mg/100 g	662:674	Total phenolics (1293 mg gallic acid equivalent/100 g) and total anthocyanins (1045 mg/100 g) contents were higher in the peel, with the major anthocyanin identified using HPLC-DAD-MS/MS as cyanidin 3-glucoside.
27352306	7	58	theme	antioxidant	1199:1209	arg1	compounds					1211:1219	antioxidant compounds	1199:1219	antioxidant compounds with potential benefits to human health	1199:1259	This study highlights the potential of Malay apple fruit as a good source of antioxidant compounds with potential benefits to human health.
27352306	0	59	theme	Malay	83:87	arg1	Merr					121:124	Malay Apple [Syzygium malaccense (L.) Merr	83:124	Malay Apple [Syzygium malaccense (L.) Merr	83:124	Physico-Chemical Characterization, Bioactive Compounds and Antioxidant Activity of Malay Apple [Syzygium malaccense (L.) Merr.
27352306	5	60	theme	Higher	795:800	arg1	values					802:807	Higher values	795:807	Higher values for DPPH antiradical scavenging activity (47.52 μMol trolox equivalent antioxidant capacity/g) and Ferric Reducing Antioxidant Potential (FRAP, 0.19 mM ferreous sulfate/g)	795:979	Higher values for DPPH antiradical scavenging activity (47.52 μMol trolox equivalent antioxidant capacity/g) and Ferric Reducing Antioxidant Potential (FRAP, 0.19 mM ferreous sulfate/g) were also observed in the peel fraction.
27352306	4	61	dep	acid	615:618	arg1	g					635:635	equivalent/100 g	620:635	1293 mg gallic acid equivalent/100 g	600:635	Total phenolics (1293 mg gallic acid equivalent/100 g) and total anthocyanins (1045 mg/100 g) contents were higher in the peel, with the major anthocyanin identified using HPLC-DAD-MS/MS as cyanidin 3-glucoside.
27352306	7	62	theme	human	1248:1252	arg1	health					1254:1259	human health	1248:1259	human health	1248:1259	This study highlights the potential of Malay apple fruit as a good source of antioxidant compounds with potential benefits to human health.
27352306	2	63	theme	geographical	345:356	arg1	origin					358:363	the geographical origin	341:363	the geographical origin	341:363	The purpose of this study was to evaluate the physico-chemical characteristics, bioactive compounds and antioxidant activity of Malay apple fruit (Syzygium malaccense) grown in Brazil with regard to the geographical origin and its peel fractions and edible portion analyzed independently.
27352306	4	64	theme	gallic	608:613	arg1	acid					615:618	1293 mg gallic acid	600:618	1293 mg gallic acid equivalent/100 g	600:635	Total phenolics (1293 mg gallic acid equivalent/100 g) and total anthocyanins (1045 mg/100 g) contents were higher in the peel, with the major anthocyanin identified using HPLC-DAD-MS/MS as cyanidin 3-glucoside.
27352306	4	65	theme	Total	583:587	arg1	phenolics					589:597	Total phenolics	583:597	Total phenolics (1293 mg gallic acid equivalent/100 g)	583:636	Total phenolics (1293 mg gallic acid equivalent/100 g) and total anthocyanins (1045 mg/100 g) contents were higher in the peel, with the major anthocyanin identified using HPLC-DAD-MS/MS as cyanidin 3-glucoside.
27352306	4	66	theme	major	720:724	arg1	anthocyanin					726:736	the major anthocyanin	716:736	the major anthocyanin identified using HPLC-DAD-MS/MS as cyanidin 3-glucoside	716:792	Total phenolics (1293 mg gallic acid equivalent/100 g) and total anthocyanins (1045 mg/100 g) contents were higher in the peel, with the major anthocyanin identified using HPLC-DAD-MS/MS as cyanidin 3-glucoside.
28822024	4	0	theme	CS-type	957:963	arg1	A					965:965	CS-type A	957:965	CS-type A	957:965	In regards to CS/DS, the level of 4S disaccharide (the major component of CS-type A and DS) decreased and the level of 6S disaccharide (the major component of CS- type C) increased in cancer tissues.
28822024	3	1	theme	several	638:644	arg1	components					665:674	several minor disaccharide components	638:674	several minor disaccharide components	638:674	In HS, several minor disaccharide components, including NS6S, NS2S and 2S were significantly lower in cancer tissues, while the levels of major disaccharides, TriS, NS and 0S disaccharides were not significantly different in normal and cancer tissues.
28822024	3	1	theme	several	638:644	arg1	NS6S					687:690	NS6S	687:690	NS6S	687:690	In HS, several minor disaccharide components, including NS6S, NS2S and 2S were significantly lower in cancer tissues, while the levels of major disaccharides, TriS, NS and 0S disaccharides were not significantly different in normal and cancer tissues.
28822024	4	2	dep	disaccharide	920:931	arg1	component					944:952	the major component	934:952	the major component of CS-type A and DS	934:972	In regards to CS/DS, the level of 4S disaccharide (the major component of CS-type A and DS) decreased and the level of 6S disaccharide (the major component of CS- type C) increased in cancer tissues.
28822024	2	3	theme	cancer	375:380	arg1	samples					389:395	The cancer tissue samples	371:395	The cancer tissue samples	371:395	The cancer tissue samples contained over twice as much chondroitin sulfate (CS)/dermatan sulfate (DS) as did the normal tissue samples, while the amount of heparan sulfate (HS) and hyaluronan (HA) in normal and cancer tissues were not significantly different.
28822024	6	4	theme	cancer	1317:1322	arg1	tissues					1324:1330	cancer tissues	1317:1330	cancer tissues	1317:1330	Analysis of the glycolipids showed all lipids present in these lung tissues, with the exception of sphingomyelin were higher in cancer tissues than in normal tissues.
28822024	1	5	theme	patient-matched	285:299	arg1	tissues					327:333	patient-matched normal and cancerous lung tissues	285:333	patient-matched normal and cancerous lung tissues obtained from lung cancer patients	285:368	In this report, we used liquid chromatography-mass spectrometry and Western blotting to analyze the content and structure of glycosaminoglycans, glycolipids and selected proteins to compare differences between patient-matched normal and cancerous lung tissues obtained from lung cancer patients.
28822024	5	6	from	structure	1116:1124	arg1	patients					1179:1186	smoking and non-smoking patients	1155:1186	smoking and non-smoking patients	1155:1186	We also compared the content and structure of GAGs in lung tissues from smoking and non-smoking patients.
28822024	5	6	from	structure	1116:1124	arg1	tissues					1142:1148	lung tissues	1137:1148	lung tissues from smoking and non-smoking patients	1137:1186	We also compared the content and structure of GAGs in lung tissues from smoking and non-smoking patients.
28822024	4	7	theme	major	1023:1027	arg1	component					1029:1037	the major component	1019:1037	the major component of CS- type C	1019:1051	In regards to CS/DS, the level of 4S disaccharide (the major component of CS-type A and DS) decreased and the level of 6S disaccharide (the major component of CS- type C) increased in cancer tissues.
28822024	1	8	theme	glycosaminoglycans	200:217	arg1	structure					187:195	structure	187:195	structure	187:195	In this report, we used liquid chromatography-mass spectrometry and Western blotting to analyze the content and structure of glycosaminoglycans, glycolipids and selected proteins to compare differences between patient-matched normal and cancerous lung tissues obtained from lung cancer patients.
28822024	1	8	theme	glycosaminoglycans	200:217	arg1	content					175:181	content	175:181	content	175:181	In this report, we used liquid chromatography-mass spectrometry and Western blotting to analyze the content and structure of glycosaminoglycans, glycolipids and selected proteins to compare differences between patient-matched normal and cancerous lung tissues obtained from lung cancer patients.
28822024	2	9	theme	heparan	527:533	arg1	sulfate					535:541	heparan sulfate	527:541	heparan sulfate (HS)	527:546	The cancer tissue samples contained over twice as much chondroitin sulfate (CS)/dermatan sulfate (DS) as did the normal tissue samples, while the amount of heparan sulfate (HS) and hyaluronan (HA) in normal and cancer tissues were not significantly different.
28822024	2	9	theme	heparan	527:533	arg1	HS					544:545	HS	544:545	HS	544:545	The cancer tissue samples contained over twice as much chondroitin sulfate (CS)/dermatan sulfate (DS) as did the normal tissue samples, while the amount of heparan sulfate (HS) and hyaluronan (HA) in normal and cancer tissues were not significantly different.
28822024	5	10	from	patients	1179:1186	arg1	tissues					1142:1148	lung tissues	1137:1148	lung tissues from smoking and non-smoking patients	1137:1186	We also compared the content and structure of GAGs in lung tissues from smoking and non-smoking patients.
28822024	5	10	from	patients	1179:1186	arg1	structure					1116:1124	structure	1116:1124	structure	1116:1124	We also compared the content and structure of GAGs in lung tissues from smoking and non-smoking patients.
28822024	5	10	from	patients	1179:1186	arg1	content					1104:1110	content	1104:1110	content	1104:1110	We also compared the content and structure of GAGs in lung tissues from smoking and non-smoking patients.
28822024	4	11	theme	6S	1002:1003	arg1	disaccharide					1005:1016	6S disaccharide	1002:1016	6S disaccharide (the major component of CS- type C)	1002:1052	In regards to CS/DS, the level of 4S disaccharide (the major component of CS-type A and DS) decreased and the level of 6S disaccharide (the major component of CS- type C) increased in cancer tissues.
28822024	4	12	dep	disaccharide	1005:1016	arg1	component					1029:1037	the major component	1019:1037	the major component of CS- type C	1019:1051	In regards to CS/DS, the level of 4S disaccharide (the major component of CS-type A and DS) decreased and the level of 6S disaccharide (the major component of CS- type C) increased in cancer tissues.
28822024	1	13	theme	glycolipids	220:230	arg1	structure					187:195	structure	187:195	structure	187:195	In this report, we used liquid chromatography-mass spectrometry and Western blotting to analyze the content and structure of glycosaminoglycans, glycolipids and selected proteins to compare differences between patient-matched normal and cancerous lung tissues obtained from lung cancer patients.
28822024	1	13	theme	glycolipids	220:230	arg1	content					175:181	content	175:181	content	175:181	In this report, we used liquid chromatography-mass spectrometry and Western blotting to analyze the content and structure of glycosaminoglycans, glycolipids and selected proteins to compare differences between patient-matched normal and cancerous lung tissues obtained from lung cancer patients.
28822024	2	14	theme	hyaluronan	552:561	arg1	HS					544:545	HS	544:545	HS	544:545	The cancer tissue samples contained over twice as much chondroitin sulfate (CS)/dermatan sulfate (DS) as did the normal tissue samples, while the amount of heparan sulfate (HS) and hyaluronan (HA) in normal and cancer tissues were not significantly different.
28822024	2	14	theme	hyaluronan	552:561	arg1	amount					517:522	the amount	513:522	the amount of heparan sulfate (HS) and hyaluronan (HA) in normal and cancer tissues	513:595	The cancer tissue samples contained over twice as much chondroitin sulfate (CS)/dermatan sulfate (DS) as did the normal tissue samples, while the amount of heparan sulfate (HS) and hyaluronan (HA) in normal and cancer tissues were not significantly different.
28822024	2	14	theme	hyaluronan	552:561	arg1	different					620:628	different	620:628	different	620:628	The cancer tissue samples contained over twice as much chondroitin sulfate (CS)/dermatan sulfate (DS) as did the normal tissue samples, while the amount of heparan sulfate (HS) and hyaluronan (HA) in normal and cancer tissues were not significantly different.
28822024	2	14	theme	hyaluronan	552:561	arg1	hyaluronan					552:561	hyaluronan	552:561	hyaluronan (HA)	552:566	The cancer tissue samples contained over twice as much chondroitin sulfate (CS)/dermatan sulfate (DS) as did the normal tissue samples, while the amount of heparan sulfate (HS) and hyaluronan (HA) in normal and cancer tissues were not significantly different.
28822024	2	14	theme	hyaluronan	552:561	arg1	sulfate					535:541	heparan sulfate	527:541	heparan sulfate (HS)	527:546	The cancer tissue samples contained over twice as much chondroitin sulfate (CS)/dermatan sulfate (DS) as did the normal tissue samples, while the amount of heparan sulfate (HS) and hyaluronan (HA) in normal and cancer tissues were not significantly different.
28822024	2	14	theme	hyaluronan	552:561	arg1	HA					564:565	HA	564:565	HA	564:565	The cancer tissue samples contained over twice as much chondroitin sulfate (CS)/dermatan sulfate (DS) as did the normal tissue samples, while the amount of heparan sulfate (HS) and hyaluronan (HA) in normal and cancer tissues were not significantly different.
28822024	3	15	from	cancer	867:872	arg1	different					843:851	different	843:851	different	843:851	In HS, several minor disaccharide components, including NS6S, NS2S and 2S were significantly lower in cancer tissues, while the levels of major disaccharides, TriS, NS and 0S disaccharides were not significantly different in normal and cancer tissues.
28822024	3	15	from	cancer	867:872	arg1	levels					759:764	the levels	755:764	the levels of major disaccharides, TriS, NS and 0S disaccharides	755:818	In HS, several minor disaccharide components, including NS6S, NS2S and 2S were significantly lower in cancer tissues, while the levels of major disaccharides, TriS, NS and 0S disaccharides were not significantly different in normal and cancer tissues.
28822024	1	16	theme	chromatography-mass	106:124	arg1	spectrometry					126:137	liquid chromatography-mass spectrometry	99:137	liquid chromatography-mass spectrometry	99:137	In this report, we used liquid chromatography-mass spectrometry and Western blotting to analyze the content and structure of glycosaminoglycans, glycolipids and selected proteins to compare differences between patient-matched normal and cancerous lung tissues obtained from lung cancer patients.
28822024	3	17	dep	normal	856:861	arg1	tissues					874:880	tissues	874:880	tissues	874:880	In HS, several minor disaccharide components, including NS6S, NS2S and 2S were significantly lower in cancer tissues, while the levels of major disaccharides, TriS, NS and 0S disaccharides were not significantly different in normal and cancer tissues.
28822024	7	18	theme	tissue/biopsy	1459:1471	arg1	samples					1473:1479	cancer tissue/biopsy samples	1452:1479	cancer tissue/biopsy samples	1452:1479	Western analysis showed that syndecan 1 and 2 proteoglycans displayed much higher expression in cancer tissue/biopsy samples.
28822024	3	19	from	different	843:851	arg1	cancer					867:872	cancer	867:872	cancer	867:872	In HS, several minor disaccharide components, including NS6S, NS2S and 2S were significantly lower in cancer tissues, while the levels of major disaccharides, TriS, NS and 0S disaccharides were not significantly different in normal and cancer tissues.
28822024	3	19	from	different	843:851	arg1	normal					856:861	normal	856:861	normal	856:861	In HS, several minor disaccharide components, including NS6S, NS2S and 2S were significantly lower in cancer tissues, while the levels of major disaccharides, TriS, NS and 0S disaccharides were not significantly different in normal and cancer tissues.
28822024	5	20	theme	smoking	1155:1161	arg1	patients					1179:1186	smoking and non-smoking patients	1155:1186	smoking and non-smoking patients	1155:1186	We also compared the content and structure of GAGs in lung tissues from smoking and non-smoking patients.
28822024	6	21	theme	lung	1252:1255	arg1	tissues					1257:1263	these lung tissues	1246:1263	these lung tissues	1246:1263	Analysis of the glycolipids showed all lipids present in these lung tissues, with the exception of sphingomyelin were higher in cancer tissues than in normal tissues.
28822024	1	22	theme	cancer	354:359	arg1	patients					361:368	lung cancer patients	349:368	lung cancer patients	349:368	In this report, we used liquid chromatography-mass spectrometry and Western blotting to analyze the content and structure of glycosaminoglycans, glycolipids and selected proteins to compare differences between patient-matched normal and cancerous lung tissues obtained from lung cancer patients.
28822024	3	23	theme	disaccharides	806:818	arg1	different					843:851	different	843:851	different	843:851	In HS, several minor disaccharide components, including NS6S, NS2S and 2S were significantly lower in cancer tissues, while the levels of major disaccharides, TriS, NS and 0S disaccharides were not significantly different in normal and cancer tissues.
28822024	3	23	theme	disaccharides	806:818	arg1	levels					759:764	the levels	755:764	the levels of major disaccharides, TriS, NS and 0S disaccharides	755:818	In HS, several minor disaccharide components, including NS6S, NS2S and 2S were significantly lower in cancer tissues, while the levels of major disaccharides, TriS, NS and 0S disaccharides were not significantly different in normal and cancer tissues.
28822024	2	24	theme	normal	484:489	arg1	samples					498:504	the normal tissue samples	480:504	the normal tissue samples	480:504	The cancer tissue samples contained over twice as much chondroitin sulfate (CS)/dermatan sulfate (DS) as did the normal tissue samples, while the amount of heparan sulfate (HS) and hyaluronan (HA) in normal and cancer tissues were not significantly different.
28822024	4	25	theme	type	1046:1049	arg1	C					1051:1051	CS- type C	1042:1051	CS- type C	1042:1051	In regards to CS/DS, the level of 4S disaccharide (the major component of CS-type A and DS) decreased and the level of 6S disaccharide (the major component of CS- type C) increased in cancer tissues.
28822024	3	26	theme	major	769:773	arg1	disaccharides					775:787	major disaccharides	769:787	major disaccharides	769:787	In HS, several minor disaccharide components, including NS6S, NS2S and 2S were significantly lower in cancer tissues, while the levels of major disaccharides, TriS, NS and 0S disaccharides were not significantly different in normal and cancer tissues.
28822024	6	27	from	tissues	1257:1263	arg1	present					1235:1241	present	1235:1241	present	1235:1241	Analysis of the glycolipids showed all lipids present in these lung tissues, with the exception of sphingomyelin were higher in cancer tissues than in normal tissues.
28822024	7	28	theme	higher	1431:1436	arg1	expression					1438:1447	much higher expression	1426:1447	much higher expression in cancer tissue/biopsy samples	1426:1479	Western analysis showed that syndecan 1 and 2 proteoglycans displayed much higher expression in cancer tissue/biopsy samples.
28822024	6	29	attach	present	1235:1241	arg1	tissues					1257:1263	these lung tissues	1246:1263	these lung tissues	1246:1263	Analysis of the glycolipids showed all lipids present in these lung tissues, with the exception of sphingomyelin were higher in cancer tissues than in normal tissues.
28822024	6	29	attach	present	1235:1241	arg2	lipids					1228:1233	all lipids	1224:1233	all lipids present in these lung tissues	1224:1263	Analysis of the glycolipids showed all lipids present in these lung tissues, with the exception of sphingomyelin were higher in cancer tissues than in normal tissues.
28822024	8	30	from	roles	1559:1563	arg1	glycosaminoglycans					1568:1585	glycosaminoglycans	1568:1585	glycosaminoglycans	1568:1585	This investigation begins to provide an understanding of patho-physiological roles on glycosaminoglycans and glycolipids and might be useful in identifying potential biomarkers in lung cancer.
28822024	8	30	from	roles	1559:1563	arg1	glycolipids					1591:1601	glycolipids	1591:1601	glycolipids	1591:1601	This investigation begins to provide an understanding of patho-physiological roles on glycosaminoglycans and glycolipids and might be useful in identifying potential biomarkers in lung cancer.
28822024	2	31	theme	much	421:424	arg1	sulfate					460:466	much chondroitin sulfate (CS)/dermatan sulfate	421:466	much chondroitin sulfate (CS)/dermatan sulfate (DS)	421:471	The cancer tissue samples contained over twice as much chondroitin sulfate (CS)/dermatan sulfate (DS) as did the normal tissue samples, while the amount of heparan sulfate (HS) and hyaluronan (HA) in normal and cancer tissues were not significantly different.
28822024	2	31	theme	much	421:424	arg1	DS					469:470	DS	469:470	DS	469:470	The cancer tissue samples contained over twice as much chondroitin sulfate (CS)/dermatan sulfate (DS) as did the normal tissue samples, while the amount of heparan sulfate (HS) and hyaluronan (HA) in normal and cancer tissues were not significantly different.
28822024	2	31	theme	much	421:424	arg1	CS					447:448	CS	447:448	CS	447:448	The cancer tissue samples contained over twice as much chondroitin sulfate (CS)/dermatan sulfate (DS) as did the normal tissue samples, while the amount of heparan sulfate (HS) and hyaluronan (HA) in normal and cancer tissues were not significantly different.
28822024	6	32	from	present	1235:1241	arg1	tissues					1257:1263	these lung tissues	1246:1263	these lung tissues	1246:1263	Analysis of the glycolipids showed all lipids present in these lung tissues, with the exception of sphingomyelin were higher in cancer tissues than in normal tissues.
28822024	4	33	theme	major	938:942	arg1	component					944:952	the major component	934:952	the major component of CS-type A and DS	934:972	In regards to CS/DS, the level of 4S disaccharide (the major component of CS-type A and DS) decreased and the level of 6S disaccharide (the major component of CS- type C) increased in cancer tissues.
28822024	8	34	from	glycolipids	1591:1601	arg1	understanding					1522:1534	an understanding	1519:1534	an understanding of patho-physiological roles on glycosaminoglycans and glycolipids	1519:1601	This investigation begins to provide an understanding of patho-physiological roles on glycosaminoglycans and glycolipids and might be useful in identifying potential biomarkers in lung cancer.
28822024	1	35	theme	normal	301:306	arg1	tissues					327:333	patient-matched normal and cancerous lung tissues	285:333	patient-matched normal and cancerous lung tissues obtained from lung cancer patients	285:368	In this report, we used liquid chromatography-mass spectrometry and Western blotting to analyze the content and structure of glycosaminoglycans, glycolipids and selected proteins to compare differences between patient-matched normal and cancerous lung tissues obtained from lung cancer patients.
28822024	2	36	theme	/dermatan	450:458	arg1	sulfate					460:466	much chondroitin sulfate (CS)/dermatan sulfate	421:466	much chondroitin sulfate (CS)/dermatan sulfate (DS)	421:471	The cancer tissue samples contained over twice as much chondroitin sulfate (CS)/dermatan sulfate (DS) as did the normal tissue samples, while the amount of heparan sulfate (HS) and hyaluronan (HA) in normal and cancer tissues were not significantly different.
28822024	2	36	theme	/dermatan	450:458	arg1	DS					469:470	DS	469:470	DS	469:470	The cancer tissue samples contained over twice as much chondroitin sulfate (CS)/dermatan sulfate (DS) as did the normal tissue samples, while the amount of heparan sulfate (HS) and hyaluronan (HA) in normal and cancer tissues were not significantly different.
28822024	2	36	theme	/dermatan	450:458	arg1	CS					447:448	CS	447:448	CS	447:448	The cancer tissue samples contained over twice as much chondroitin sulfate (CS)/dermatan sulfate (DS) as did the normal tissue samples, while the amount of heparan sulfate (HS) and hyaluronan (HA) in normal and cancer tissues were not significantly different.
28822024	8	37	from	glycosaminoglycans	1568:1585	arg1	understanding					1522:1534	an understanding	1519:1534	an understanding of patho-physiological roles on glycosaminoglycans and glycolipids	1519:1601	This investigation begins to provide an understanding of patho-physiological roles on glycosaminoglycans and glycolipids and might be useful in identifying potential biomarkers in lung cancer.
28822024	5	38	theme	non-smoking	1167:1177	arg1	patients					1179:1186	smoking and non-smoking patients	1155:1186	smoking and non-smoking patients	1155:1186	We also compared the content and structure of GAGs in lung tissues from smoking and non-smoking patients.
28822024	1	39	theme	cancerous	312:320	arg1	tissues					327:333	patient-matched normal and cancerous lung tissues	285:333	patient-matched normal and cancerous lung tissues obtained from lung cancer patients	285:368	In this report, we used liquid chromatography-mass spectrometry and Western blotting to analyze the content and structure of glycosaminoglycans, glycolipids and selected proteins to compare differences between patient-matched normal and cancerous lung tissues obtained from lung cancer patients.
28822024	4	40	theme	4S	917:918	arg1	disaccharide					920:931	4S disaccharide	917:931	4S disaccharide (the major component of CS-type A and DS)	917:973	In regards to CS/DS, the level of 4S disaccharide (the major component of CS-type A and DS) decreased and the level of 6S disaccharide (the major component of CS- type C) increased in cancer tissues.
28822024	2	41	theme	sulfate	438:444	arg1	sulfate					460:466	much chondroitin sulfate (CS)/dermatan sulfate	421:466	much chondroitin sulfate (CS)/dermatan sulfate (DS)	421:471	The cancer tissue samples contained over twice as much chondroitin sulfate (CS)/dermatan sulfate (DS) as did the normal tissue samples, while the amount of heparan sulfate (HS) and hyaluronan (HA) in normal and cancer tissues were not significantly different.
28822024	2	41	theme	sulfate	438:444	arg1	DS					469:470	DS	469:470	DS	469:470	The cancer tissue samples contained over twice as much chondroitin sulfate (CS)/dermatan sulfate (DS) as did the normal tissue samples, while the amount of heparan sulfate (HS) and hyaluronan (HA) in normal and cancer tissues were not significantly different.
28822024	2	41	theme	sulfate	438:444	arg1	CS					447:448	CS	447:448	CS	447:448	The cancer tissue samples contained over twice as much chondroitin sulfate (CS)/dermatan sulfate (DS) as did the normal tissue samples, while the amount of heparan sulfate (HS) and hyaluronan (HA) in normal and cancer tissues were not significantly different.
28822024	0	42	theme	potential	38:46	arg1	biomarkers					48:57	potential biomarkers	38:57	potential biomarkers in lung cancer	38:72	Glycosaminoglycans and glycolipids as potential biomarkers in lung cancer.
28822024	7	43	theme	syndecan	1385:1392	arg1	proteoglycans					1402:1414	syndecan 1 and 2 proteoglycans	1385:1414	syndecan 1 and 2 proteoglycans	1385:1414	Western analysis showed that syndecan 1 and 2 proteoglycans displayed much higher expression in cancer tissue/biopsy samples.
28822024	8	44	from	biomarkers	1648:1657	arg1	cancer					1667:1672	lung cancer	1662:1672	lung cancer	1662:1672	This investigation begins to provide an understanding of patho-physiological roles on glycosaminoglycans and glycolipids and might be useful in identifying potential biomarkers in lung cancer.
28822024	7	45	theme	Western	1356:1362	arg1	analysis					1364:1371	Western analysis	1356:1371	Western analysis	1356:1371	Western analysis showed that syndecan 1 and 2 proteoglycans displayed much higher expression in cancer tissue/biopsy samples.
28822024	8	46	theme	patho-physiological	1539:1557	arg1	roles					1559:1563	patho-physiological roles	1539:1563	patho-physiological roles on glycosaminoglycans and glycolipids	1539:1601	This investigation begins to provide an understanding of patho-physiological roles on glycosaminoglycans and glycolipids and might be useful in identifying potential biomarkers in lung cancer.
28822024	1	47	theme	proteins	245:252	arg1	structure					187:195	structure	187:195	structure	187:195	In this report, we used liquid chromatography-mass spectrometry and Western blotting to analyze the content and structure of glycosaminoglycans, glycolipids and selected proteins to compare differences between patient-matched normal and cancerous lung tissues obtained from lung cancer patients.
28822024	1	47	theme	proteins	245:252	arg1	content					175:181	content	175:181	content	175:181	In this report, we used liquid chromatography-mass spectrometry and Western blotting to analyze the content and structure of glycosaminoglycans, glycolipids and selected proteins to compare differences between patient-matched normal and cancerous lung tissues obtained from lung cancer patients.
28822024	4	48	theme	A	965:965	arg1	component					944:952	the major component	934:952	the major component of CS-type A and DS	934:972	In regards to CS/DS, the level of 4S disaccharide (the major component of CS-type A and DS) decreased and the level of 6S disaccharide (the major component of CS- type C) increased in cancer tissues.
28822024	2	49	theme	tissue	382:387	arg1	samples					389:395	The cancer tissue samples	371:395	The cancer tissue samples	371:395	The cancer tissue samples contained over twice as much chondroitin sulfate (CS)/dermatan sulfate (DS) as did the normal tissue samples, while the amount of heparan sulfate (HS) and hyaluronan (HA) in normal and cancer tissues were not significantly different.
28822024	4	50	theme	DS	971:972	arg1	component					944:952	the major component	934:952	the major component of CS-type A and DS	934:972	In regards to CS/DS, the level of 4S disaccharide (the major component of CS-type A and DS) decreased and the level of 6S disaccharide (the major component of CS- type C) increased in cancer tissues.
28822024	3	51	theme	minor	646:650	arg1	components					665:674	several minor disaccharide components	638:674	several minor disaccharide components	638:674	In HS, several minor disaccharide components, including NS6S, NS2S and 2S were significantly lower in cancer tissues, while the levels of major disaccharides, TriS, NS and 0S disaccharides were not significantly different in normal and cancer tissues.
28822024	3	51	theme	minor	646:650	arg1	NS6S					687:690	NS6S	687:690	NS6S	687:690	In HS, several minor disaccharide components, including NS6S, NS2S and 2S were significantly lower in cancer tissues, while the levels of major disaccharides, TriS, NS and 0S disaccharides were not significantly different in normal and cancer tissues.
28822024	2	52	theme	cancer	582:587	arg1	tissues					589:595	cancer tissues	582:595	cancer tissues	582:595	The cancer tissue samples contained over twice as much chondroitin sulfate (CS)/dermatan sulfate (DS) as did the normal tissue samples, while the amount of heparan sulfate (HS) and hyaluronan (HA) in normal and cancer tissues were not significantly different.
28822024	6	53	theme	glycolipids	1205:1215	arg1	Analysis					1189:1196	Analysis	1189:1196	Analysis of the glycolipids	1189:1215	Analysis of the glycolipids showed all lipids present in these lung tissues, with the exception of sphingomyelin were higher in cancer tissues than in normal tissues.
28822024	2	54	theme	sulfate	535:541	arg1	HS					544:545	HS	544:545	HS	544:545	The cancer tissue samples contained over twice as much chondroitin sulfate (CS)/dermatan sulfate (DS) as did the normal tissue samples, while the amount of heparan sulfate (HS) and hyaluronan (HA) in normal and cancer tissues were not significantly different.
28822024	2	54	theme	sulfate	535:541	arg1	amount					517:522	the amount	513:522	the amount of heparan sulfate (HS) and hyaluronan (HA) in normal and cancer tissues	513:595	The cancer tissue samples contained over twice as much chondroitin sulfate (CS)/dermatan sulfate (DS) as did the normal tissue samples, while the amount of heparan sulfate (HS) and hyaluronan (HA) in normal and cancer tissues were not significantly different.
28822024	2	54	theme	sulfate	535:541	arg1	different					620:628	different	620:628	different	620:628	The cancer tissue samples contained over twice as much chondroitin sulfate (CS)/dermatan sulfate (DS) as did the normal tissue samples, while the amount of heparan sulfate (HS) and hyaluronan (HA) in normal and cancer tissues were not significantly different.
28822024	2	54	theme	sulfate	535:541	arg1	hyaluronan					552:561	hyaluronan	552:561	hyaluronan (HA)	552:566	The cancer tissue samples contained over twice as much chondroitin sulfate (CS)/dermatan sulfate (DS) as did the normal tissue samples, while the amount of heparan sulfate (HS) and hyaluronan (HA) in normal and cancer tissues were not significantly different.
28822024	2	54	theme	sulfate	535:541	arg1	sulfate					535:541	heparan sulfate	527:541	heparan sulfate (HS)	527:546	The cancer tissue samples contained over twice as much chondroitin sulfate (CS)/dermatan sulfate (DS) as did the normal tissue samples, while the amount of heparan sulfate (HS) and hyaluronan (HA) in normal and cancer tissues were not significantly different.
28822024	2	54	theme	sulfate	535:541	arg1	HA					564:565	HA	564:565	HA	564:565	The cancer tissue samples contained over twice as much chondroitin sulfate (CS)/dermatan sulfate (DS) as did the normal tissue samples, while the amount of heparan sulfate (HS) and hyaluronan (HA) in normal and cancer tissues were not significantly different.
28822024	5	55	dep	content	1104:1110	arg1	the					1100:1102	the	1100:1102	the	1100:1102	We also compared the content and structure of GAGs in lung tissues from smoking and non-smoking patients.
28822024	4	56	theme	disaccharide	1005:1016	arg1	level					993:997	the level	989:997	the level of 6S disaccharide (the major component of CS- type C)	989:1052	In regards to CS/DS, the level of 4S disaccharide (the major component of CS-type A and DS) decreased and the level of 6S disaccharide (the major component of CS- type C) increased in cancer tissues.
28822024	8	57	theme	potential	1638:1646	arg1	biomarkers					1648:1657	potential biomarkers	1638:1657	potential biomarkers in lung cancer	1638:1672	This investigation begins to provide an understanding of patho-physiological roles on glycosaminoglycans and glycolipids and might be useful in identifying potential biomarkers in lung cancer.
28822024	3	58	theme	cancer	733:738	arg1	tissues					740:746	cancer tissues	733:746	cancer tissues	733:746	In HS, several minor disaccharide components, including NS6S, NS2S and 2S were significantly lower in cancer tissues, while the levels of major disaccharides, TriS, NS and 0S disaccharides were not significantly different in normal and cancer tissues.
28822024	5	59	from	content	1104:1110	arg1	patients					1179:1186	smoking and non-smoking patients	1155:1186	smoking and non-smoking patients	1155:1186	We also compared the content and structure of GAGs in lung tissues from smoking and non-smoking patients.
28822024	5	59	from	content	1104:1110	arg1	tissues					1142:1148	lung tissues	1137:1148	lung tissues from smoking and non-smoking patients	1137:1186	We also compared the content and structure of GAGs in lung tissues from smoking and non-smoking patients.
28822024	1	60	theme	liquid	99:104	arg1	spectrometry					126:137	liquid chromatography-mass spectrometry	99:137	liquid chromatography-mass spectrometry	99:137	In this report, we used liquid chromatography-mass spectrometry and Western blotting to analyze the content and structure of glycosaminoglycans, glycolipids and selected proteins to compare differences between patient-matched normal and cancerous lung tissues obtained from lung cancer patients.
28822024	4	61	theme	cancer	1067:1072	arg1	tissues					1074:1080	cancer tissues	1067:1080	cancer tissues	1067:1080	In regards to CS/DS, the level of 4S disaccharide (the major component of CS-type A and DS) decreased and the level of 6S disaccharide (the major component of CS- type C) increased in cancer tissues.
28822024	5	62	theme	lung	1137:1140	arg1	tissues					1142:1148	lung tissues	1137:1148	lung tissues from smoking and non-smoking patients	1137:1186	We also compared the content and structure of GAGs in lung tissues from smoking and non-smoking patients.
28822024	3	63	theme	NS	796:797	arg1	disaccharides					806:818	major disaccharides, TriS, NS and 0S disaccharides	769:818	major disaccharides, TriS, NS and 0S disaccharides	769:818	In HS, several minor disaccharide components, including NS6S, NS2S and 2S were significantly lower in cancer tissues, while the levels of major disaccharides, TriS, NS and 0S disaccharides were not significantly different in normal and cancer tissues.
28822024	7	64	theme	cancer	1452:1457	arg1	samples					1473:1479	cancer tissue/biopsy samples	1452:1479	cancer tissue/biopsy samples	1452:1479	Western analysis showed that syndecan 1 and 2 proteoglycans displayed much higher expression in cancer tissue/biopsy samples.
28822024	2	65	from	amount	517:522	arg1	normal					571:576	normal	571:576	normal	571:576	The cancer tissue samples contained over twice as much chondroitin sulfate (CS)/dermatan sulfate (DS) as did the normal tissue samples, while the amount of heparan sulfate (HS) and hyaluronan (HA) in normal and cancer tissues were not significantly different.
28822024	2	65	from	amount	517:522	arg1	tissues					589:595	cancer tissues	582:595	cancer tissues	582:595	The cancer tissue samples contained over twice as much chondroitin sulfate (CS)/dermatan sulfate (DS) as did the normal tissue samples, while the amount of heparan sulfate (HS) and hyaluronan (HA) in normal and cancer tissues were not significantly different.
28822024	4	66	theme	CS-	1042:1044	arg1	C					1051:1051	CS- type C	1042:1051	CS- type C	1042:1051	In regards to CS/DS, the level of 4S disaccharide (the major component of CS-type A and DS) decreased and the level of 6S disaccharide (the major component of CS- type C) increased in cancer tissues.
28822024	1	67	theme	Western	143:149	arg1	blotting					151:158	Western blotting	143:158	Western blotting	143:158	In this report, we used liquid chromatography-mass spectrometry and Western blotting to analyze the content and structure of glycosaminoglycans, glycolipids and selected proteins to compare differences between patient-matched normal and cancerous lung tissues obtained from lung cancer patients.
28822024	3	68	theme	TriS	790:793	arg1	disaccharides					806:818	major disaccharides, TriS, NS and 0S disaccharides	769:818	major disaccharides, TriS, NS and 0S disaccharides	769:818	In HS, several minor disaccharide components, including NS6S, NS2S and 2S were significantly lower in cancer tissues, while the levels of major disaccharides, TriS, NS and 0S disaccharides were not significantly different in normal and cancer tissues.
28822024	2	69	theme	tissue	491:496	arg1	samples					498:504	the normal tissue samples	480:504	the normal tissue samples	480:504	The cancer tissue samples contained over twice as much chondroitin sulfate (CS)/dermatan sulfate (DS) as did the normal tissue samples, while the amount of heparan sulfate (HS) and hyaluronan (HA) in normal and cancer tissues were not significantly different.
28822024	4	70	theme	C	1051:1051	arg1	component					1029:1037	the major component	1019:1037	the major component of CS- type C	1019:1051	In regards to CS/DS, the level of 4S disaccharide (the major component of CS-type A and DS) decreased and the level of 6S disaccharide (the major component of CS- type C) increased in cancer tissues.
28822024	5	71	theme	GAGs	1129:1132	arg1	structure					1116:1124	structure	1116:1124	structure	1116:1124	We also compared the content and structure of GAGs in lung tissues from smoking and non-smoking patients.
28822024	5	71	theme	GAGs	1129:1132	arg1	content					1104:1110	content	1104:1110	content	1104:1110	We also compared the content and structure of GAGs in lung tissues from smoking and non-smoking patients.
28822024	3	72	theme	disaccharides	775:787	arg1	disaccharides					806:818	major disaccharides, TriS, NS and 0S disaccharides	769:818	major disaccharides, TriS, NS and 0S disaccharides	769:818	In HS, several minor disaccharide components, including NS6S, NS2S and 2S were significantly lower in cancer tissues, while the levels of major disaccharides, TriS, NS and 0S disaccharides were not significantly different in normal and cancer tissues.
28822024	6	73	theme	present	1235:1241	arg1	lipids					1228:1233	all lipids	1224:1233	all lipids present in these lung tissues	1224:1263	Analysis of the glycolipids showed all lipids present in these lung tissues, with the exception of sphingomyelin were higher in cancer tissues than in normal tissues.
28822024	2	74	dep	did	476:478	arg1	as					473:474	as	473:474	as	473:474	The cancer tissue samples contained over twice as much chondroitin sulfate (CS)/dermatan sulfate (DS) as did the normal tissue samples, while the amount of heparan sulfate (HS) and hyaluronan (HA) in normal and cancer tissues were not significantly different.
28822024	3	75	theme	0S	803:804	arg1	disaccharides					806:818	major disaccharides, TriS, NS and 0S disaccharides	769:818	major disaccharides, TriS, NS and 0S disaccharides	769:818	In HS, several minor disaccharide components, including NS6S, NS2S and 2S were significantly lower in cancer tissues, while the levels of major disaccharides, TriS, NS and 0S disaccharides were not significantly different in normal and cancer tissues.
28822024	8	76	theme	roles	1559:1563	arg1	understanding					1522:1534	an understanding	1519:1534	an understanding of patho-physiological roles on glycosaminoglycans and glycolipids	1519:1601	This investigation begins to provide an understanding of patho-physiological roles on glycosaminoglycans and glycolipids and might be useful in identifying potential biomarkers in lung cancer.
28822024	0	77	from	biomarkers	48:57	arg1	cancer					67:72	lung cancer	62:72	lung cancer	62:72	Glycosaminoglycans and glycolipids as potential biomarkers in lung cancer.
28822024	8	78	from	understanding	1522:1534	arg1	glycosaminoglycans					1568:1585	glycosaminoglycans	1568:1585	glycosaminoglycans	1568:1585	This investigation begins to provide an understanding of patho-physiological roles on glycosaminoglycans and glycolipids and might be useful in identifying potential biomarkers in lung cancer.
28822024	8	78	from	understanding	1522:1534	arg1	glycolipids					1591:1601	glycolipids	1591:1601	glycolipids	1591:1601	This investigation begins to provide an understanding of patho-physiological roles on glycosaminoglycans and glycolipids and might be useful in identifying potential biomarkers in lung cancer.
28822024	6	79	theme	sphingomyelin	1288:1300	arg1	exception					1275:1283	the exception	1271:1283	the exception of sphingomyelin were higher in cancer tissues than in normal tissues	1271:1353	Analysis of the glycolipids showed all lipids present in these lung tissues, with the exception of sphingomyelin were higher in cancer tissues than in normal tissues.
28822024	3	80	from	normal	856:861	arg1	different					843:851	different	843:851	different	843:851	In HS, several minor disaccharide components, including NS6S, NS2S and 2S were significantly lower in cancer tissues, while the levels of major disaccharides, TriS, NS and 0S disaccharides were not significantly different in normal and cancer tissues.
28822024	3	80	from	normal	856:861	arg1	levels					759:764	the levels	755:764	the levels of major disaccharides, TriS, NS and 0S disaccharides	755:818	In HS, several minor disaccharide components, including NS6S, NS2S and 2S were significantly lower in cancer tissues, while the levels of major disaccharides, TriS, NS and 0S disaccharides were not significantly different in normal and cancer tissues.
28822024	1	81	theme	lung	322:325	arg1	tissues					327:333	patient-matched normal and cancerous lung tissues	285:333	patient-matched normal and cancerous lung tissues obtained from lung cancer patients	285:368	In this report, we used liquid chromatography-mass spectrometry and Western blotting to analyze the content and structure of glycosaminoglycans, glycolipids and selected proteins to compare differences between patient-matched normal and cancerous lung tissues obtained from lung cancer patients.
28822024	7	82	theme	2	1400:1400	arg1	proteoglycans					1402:1414	syndecan 1 and 2 proteoglycans	1385:1414	syndecan 1 and 2 proteoglycans	1385:1414	Western analysis showed that syndecan 1 and 2 proteoglycans displayed much higher expression in cancer tissue/biopsy samples.
28822024	2	83	theme	chondroitin	426:436	arg1	sulfate					460:466	much chondroitin sulfate (CS)/dermatan sulfate	421:466	much chondroitin sulfate (CS)/dermatan sulfate (DS)	421:471	The cancer tissue samples contained over twice as much chondroitin sulfate (CS)/dermatan sulfate (DS) as did the normal tissue samples, while the amount of heparan sulfate (HS) and hyaluronan (HA) in normal and cancer tissues were not significantly different.
28822024	2	83	theme	chondroitin	426:436	arg1	DS					469:470	DS	469:470	DS	469:470	The cancer tissue samples contained over twice as much chondroitin sulfate (CS)/dermatan sulfate (DS) as did the normal tissue samples, while the amount of heparan sulfate (HS) and hyaluronan (HA) in normal and cancer tissues were not significantly different.
28822024	2	83	theme	chondroitin	426:436	arg1	CS					447:448	CS	447:448	CS	447:448	The cancer tissue samples contained over twice as much chondroitin sulfate (CS)/dermatan sulfate (DS) as did the normal tissue samples, while the amount of heparan sulfate (HS) and hyaluronan (HA) in normal and cancer tissues were not significantly different.
28822024	0	84	theme	lung	62:65	arg1	cancer					67:72	lung cancer	62:72	lung cancer	62:72	Glycosaminoglycans and glycolipids as potential biomarkers in lung cancer.
28822024	7	85	from	expression	1438:1447	arg1	samples					1473:1479	cancer tissue/biopsy samples	1452:1479	cancer tissue/biopsy samples	1452:1479	Western analysis showed that syndecan 1 and 2 proteoglycans displayed much higher expression in cancer tissue/biopsy samples.
28822024	7	86	theme	1	1394:1394	arg1	proteoglycans					1402:1414	syndecan 1 and 2 proteoglycans	1385:1414	syndecan 1 and 2 proteoglycans	1385:1414	Western analysis showed that syndecan 1 and 2 proteoglycans displayed much higher expression in cancer tissue/biopsy samples.
28822024	1	87	used	used	94:97	arg2	we					91:92	we	91:92	we	91:92	In this report, we used liquid chromatography-mass spectrometry and Western blotting to analyze the content and structure of glycosaminoglycans, glycolipids and selected proteins to compare differences between patient-matched normal and cancerous lung tissues obtained from lung cancer patients.
28822024	4	88	theme	disaccharide	920:931	arg1	level					908:912	the level	904:912	the level of 4S disaccharide (the major component of CS-type A and DS)	904:973	In regards to CS/DS, the level of 4S disaccharide (the major component of CS-type A and DS) decreased and the level of 6S disaccharide (the major component of CS- type C) increased in cancer tissues.
28822024	1	89	dep	content	175:181	arg1	the					171:173	the	171:173	the	171:173	In this report, we used liquid chromatography-mass spectrometry and Western blotting to analyze the content and structure of glycosaminoglycans, glycolipids and selected proteins to compare differences between patient-matched normal and cancerous lung tissues obtained from lung cancer patients.
28822024	1	90	theme	lung	349:352	arg1	patients					361:368	lung cancer patients	349:368	lung cancer patients	349:368	In this report, we used liquid chromatography-mass spectrometry and Western blotting to analyze the content and structure of glycosaminoglycans, glycolipids and selected proteins to compare differences between patient-matched normal and cancerous lung tissues obtained from lung cancer patients.
28822024	2	91	dep	samples	498:504	arg1	sulfate					535:541	heparan sulfate	527:541	heparan sulfate (HS)	527:546	The cancer tissue samples contained over twice as much chondroitin sulfate (CS)/dermatan sulfate (DS) as did the normal tissue samples, while the amount of heparan sulfate (HS) and hyaluronan (HA) in normal and cancer tissues were not significantly different.
28822024	2	91	dep	samples	498:504	arg1	different					620:628	different	620:628	different	620:628	The cancer tissue samples contained over twice as much chondroitin sulfate (CS)/dermatan sulfate (DS) as did the normal tissue samples, while the amount of heparan sulfate (HS) and hyaluronan (HA) in normal and cancer tissues were not significantly different.
28822024	2	91	dep	samples	498:504	arg1	hyaluronan					552:561	hyaluronan	552:561	hyaluronan (HA)	552:566	The cancer tissue samples contained over twice as much chondroitin sulfate (CS)/dermatan sulfate (DS) as did the normal tissue samples, while the amount of heparan sulfate (HS) and hyaluronan (HA) in normal and cancer tissues were not significantly different.
28822024	2	91	dep	samples	498:504	arg1	amount					517:522	the amount	513:522	the amount of heparan sulfate (HS) and hyaluronan (HA) in normal and cancer tissues	513:595	The cancer tissue samples contained over twice as much chondroitin sulfate (CS)/dermatan sulfate (DS) as did the normal tissue samples, while the amount of heparan sulfate (HS) and hyaluronan (HA) in normal and cancer tissues were not significantly different.
28822024	8	92	theme	lung	1662:1665	arg1	cancer					1667:1672	lung cancer	1662:1672	lung cancer	1662:1672	This investigation begins to provide an understanding of patho-physiological roles on glycosaminoglycans and glycolipids and might be useful in identifying potential biomarkers in lung cancer.
28822024	6	93	theme	normal	1340:1345	arg1	tissues					1347:1353	normal tissues	1340:1353	normal tissues	1340:1353	Analysis of the glycolipids showed all lipids present in these lung tissues, with the exception of sphingomyelin were higher in cancer tissues than in normal tissues.
28822024	1	94	theme	selected	236:243	arg1	proteins					245:252	selected proteins	236:252	selected proteins	236:252	In this report, we used liquid chromatography-mass spectrometry and Western blotting to analyze the content and structure of glycosaminoglycans, glycolipids and selected proteins to compare differences between patient-matched normal and cancerous lung tissues obtained from lung cancer patients.
28822024	3	95	theme	disaccharide	652:663	arg1	components					665:674	several minor disaccharide components	638:674	several minor disaccharide components	638:674	In HS, several minor disaccharide components, including NS6S, NS2S and 2S were significantly lower in cancer tissues, while the levels of major disaccharides, TriS, NS and 0S disaccharides were not significantly different in normal and cancer tissues.
28822024	3	95	theme	disaccharide	652:663	arg1	NS6S					687:690	NS6S	687:690	NS6S	687:690	In HS, several minor disaccharide components, including NS6S, NS2S and 2S were significantly lower in cancer tissues, while the levels of major disaccharides, TriS, NS and 0S disaccharides were not significantly different in normal and cancer tissues.
29050574	4	0	theme	inorganic-hybridized	695:714	arg1	composites					720:729	the inorganic-hybridized IPN composites	691:729	the inorganic-hybridized IPN composites	691:729	SQUID magnetometry and X-ray diffractometry were conducted for evaluation of the magnetic property of the inorganic-hybridized IPN composites.
29050574	4	1	theme	composites	720:729	arg1	property					679:686	the magnetic property	666:686	the magnetic property of the inorganic-hybridized IPN composites	666:729	SQUID magnetometry and X-ray diffractometry were conducted for evaluation of the magnetic property of the inorganic-hybridized IPN composites.
29050574	1	2	theme	polymer	253:259	arg1	network					261:267	the polymer network	249:267	the polymer network	249:267	Iron oxide nanoparticles-incorporated carrageenan (CAR)/PHEMA composites of interpenetrating network (IPN) type were successfully prepared by in situ ferrite synthesis in the polymer network.
29050574	5	3	theme	Magnetite	732:740	arg1	particles					742:750	Magnetite particles	732:750	Magnetite particles with 10-30nm sizes	732:769	Magnetite particles with 10-30nm sizes were distributed inside the IPNs treated with the repeatable ferrite synthesis; thereby, the hybrids displayed a superparamagnetic character at ambient temperature.
29050574	7	4	theme	magnetic	1142:1149	arg1	material					1151:1158	a biocompatible magnetic material	1126:1158	a biocompatible magnetic material used in hydro-surroundings	1126:1185	The present IPN system offers a potential for application as a biocompatible magnetic material used in hydro-surroundings.
29050574	1	5	theme	IPN	180:182	arg1	type					185:188	interpenetrating network (IPN) type	154:188	interpenetrating network (IPN) type	154:188	Iron oxide nanoparticles-incorporated carrageenan (CAR)/PHEMA composites of interpenetrating network (IPN) type were successfully prepared by in situ ferrite synthesis in the polymer network.
29050574	5	6	theme	10-30nm	757:763	arg1	sizes					765:769	10-30nm sizes	757:769	10-30nm sizes	757:769	Magnetite particles with 10-30nm sizes were distributed inside the IPNs treated with the repeatable ferrite synthesis; thereby, the hybrids displayed a superparamagnetic character at ambient temperature.
29050574	7	7	theme	biocompatible	1128:1140	arg1	material					1151:1158	a biocompatible magnetic material	1126:1158	a biocompatible magnetic material used in hydro-surroundings	1126:1185	The present IPN system offers a potential for application as a biocompatible magnetic material used in hydro-surroundings.
29050574	5	8	theme	repeatable	821:830	arg1	synthesis					840:848	the repeatable ferrite synthesis	817:848	the repeatable ferrite synthesis	817:848	Magnetite particles with 10-30nm sizes were distributed inside the IPNs treated with the repeatable ferrite synthesis; thereby, the hybrids displayed a superparamagnetic character at ambient temperature.
29050574	4	9	theme	magnetic	670:677	arg1	property					679:686	the magnetic property	666:686	the magnetic property of the inorganic-hybridized IPN composites	666:729	SQUID magnetometry and X-ray diffractometry were conducted for evaluation of the magnetic property of the inorganic-hybridized IPN composites.
29050574	6	10	theme	g	1023:1023	arg1	sample					1025:1030	10-15emu (g sample)-1	1013:1033	10-15emu (g sample)-1	1013:1033	Specifically, the 40/60 CAR/PHEMA IPN imparted a practically passable value (10-15emu (g sample)-1) of saturation magnetization.
29050574	6	10	theme	g	1023:1023	arg1	value					1006:1010	a practically passable value	983:1010	a practically passable value (10-15emu (g sample)-1) of saturation magnetization	983:1062	Specifically, the 40/60 CAR/PHEMA IPN imparted a practically passable value (10-15emu (g sample)-1) of saturation magnetization.
29050574	3	11	theme	good	539:542	arg1	shape-retentivity					544:560	a good shape-retentivity	537:560	a good shape-retentivity	537:560	As a result of this IPN construction, the composites were firm and showed a good shape-retentivity in their gelatinous state.
29050574	3	12	theme	IPN	483:485	arg1	construction					487:498	this IPN construction	478:498	this IPN construction	478:498	As a result of this IPN construction, the composites were firm and showed a good shape-retentivity in their gelatinous state.
29050574	1	13	theme	Iron	78:81	arg1	carrageenan					116:126	Iron oxide nanoparticles-incorporated carrageenan	78:126	Iron oxide nanoparticles-incorporated carrageenan (CAR)/PHEMA composites of interpenetrating network (IPN) type	78:188	Iron oxide nanoparticles-incorporated carrageenan (CAR)/PHEMA composites of interpenetrating network (IPN) type were successfully prepared by in situ ferrite synthesis in the polymer network.
29050574	1	13	theme	Iron	78:81	arg1	CAR					129:131	CAR	129:131	CAR	129:131	Iron oxide nanoparticles-incorporated carrageenan (CAR)/PHEMA composites of interpenetrating network (IPN) type were successfully prepared by in situ ferrite synthesis in the polymer network.
29050574	2	14	theme	2-hydroxyethylmethacrylate	396:421	arg1	cross-linking					379:391	cross-linking	379:391	cross-linking of 2-hydroxyethylmethacrylate as an impregnating solvent of CAR gels	379:460	The IPN structure was constructed at CAR/PHEMA compositions of 15/85 and 40/60 (wt/wt) by polymerization and cross-linking of 2-hydroxyethylmethacrylate as an impregnating solvent of CAR gels.
29050574	2	14	theme	2-hydroxyethylmethacrylate	396:421	arg1	polymerization					360:373	polymerization	360:373	polymerization	360:373	The IPN structure was constructed at CAR/PHEMA compositions of 15/85 and 40/60 (wt/wt) by polymerization and cross-linking of 2-hydroxyethylmethacrylate as an impregnating solvent of CAR gels.
29050574	4	15	theme	SQUID	589:593	arg1	magnetometry					595:606	SQUID magnetometry	589:606	SQUID magnetometry	589:606	SQUID magnetometry and X-ray diffractometry were conducted for evaluation of the magnetic property of the inorganic-hybridized IPN composites.
29050574	1	16	theme	oxide	83:87	arg1	carrageenan					116:126	Iron oxide nanoparticles-incorporated carrageenan	78:126	Iron oxide nanoparticles-incorporated carrageenan (CAR)/PHEMA composites of interpenetrating network (IPN) type	78:188	Iron oxide nanoparticles-incorporated carrageenan (CAR)/PHEMA composites of interpenetrating network (IPN) type were successfully prepared by in situ ferrite synthesis in the polymer network.
29050574	1	16	theme	oxide	83:87	arg1	CAR					129:131	CAR	129:131	CAR	129:131	Iron oxide nanoparticles-incorporated carrageenan (CAR)/PHEMA composites of interpenetrating network (IPN) type were successfully prepared by in situ ferrite synthesis in the polymer network.
29050574	4	17	theme	X-ray	612:616	arg1	diffractometry					618:631	X-ray diffractometry	612:631	X-ray diffractometry	612:631	SQUID magnetometry and X-ray diffractometry were conducted for evaluation of the magnetic property of the inorganic-hybridized IPN composites.
29050574	1	18	theme	type	185:188	arg1	composites					140:149	Iron oxide nanoparticles-incorporated carrageenan (CAR)/PHEMA composites	78:149	Iron oxide nanoparticles-incorporated carrageenan (CAR)/PHEMA composites of interpenetrating network (IPN) type	78:188	Iron oxide nanoparticles-incorporated carrageenan (CAR)/PHEMA composites of interpenetrating network (IPN) type were successfully prepared by in situ ferrite synthesis in the polymer network.
29050574	1	18	theme	type	185:188	arg1	type					185:188	interpenetrating network (IPN) type	154:188	interpenetrating network (IPN) type	154:188	Iron oxide nanoparticles-incorporated carrageenan (CAR)/PHEMA composites of interpenetrating network (IPN) type were successfully prepared by in situ ferrite synthesis in the polymer network.
29050574	0	19	theme	IPN	18:20	arg1	gels					22:25	Superparamagnetic IPN gels	0:25	Superparamagnetic IPN gels of carrageenan/PHEMA	0:46	Superparamagnetic IPN gels of carrageenan/PHEMA excelling in shape retention.
29050574	1	20	theme	nanoparticles-incorporated	89:114	arg1	carrageenan					116:126	Iron oxide nanoparticles-incorporated carrageenan	78:126	Iron oxide nanoparticles-incorporated carrageenan (CAR)/PHEMA composites of interpenetrating network (IPN) type	78:188	Iron oxide nanoparticles-incorporated carrageenan (CAR)/PHEMA composites of interpenetrating network (IPN) type were successfully prepared by in situ ferrite synthesis in the polymer network.
29050574	1	20	theme	nanoparticles-incorporated	89:114	arg1	CAR					129:131	CAR	129:131	CAR	129:131	Iron oxide nanoparticles-incorporated carrageenan (CAR)/PHEMA composites of interpenetrating network (IPN) type were successfully prepared by in situ ferrite synthesis in the polymer network.
29050574	6	21	theme	magnetization	1050:1062	arg1	sample					1025:1030	10-15emu (g sample)-1	1013:1033	10-15emu (g sample)-1	1013:1033	Specifically, the 40/60 CAR/PHEMA IPN imparted a practically passable value (10-15emu (g sample)-1) of saturation magnetization.
29050574	6	21	theme	magnetization	1050:1062	arg1	value					1006:1010	a practically passable value	983:1010	a practically passable value (10-15emu (g sample)-1) of saturation magnetization	983:1062	Specifically, the 40/60 CAR/PHEMA IPN imparted a practically passable value (10-15emu (g sample)-1) of saturation magnetization.
29050574	0	22	theme	Superparamagnetic	0:16	arg1	gels					22:25	Superparamagnetic IPN gels	0:25	Superparamagnetic IPN gels of carrageenan/PHEMA	0:46	Superparamagnetic IPN gels of carrageenan/PHEMA excelling in shape retention.
29050574	5	23	theme	superparamagnetic	884:900	arg1	character					902:910	a superparamagnetic character	882:910	a superparamagnetic character	882:910	Magnetite particles with 10-30nm sizes were distributed inside the IPNs treated with the repeatable ferrite synthesis; thereby, the hybrids displayed a superparamagnetic character at ambient temperature.
29050574	1	24	theme	carrageenan	116:126	arg1	composites					140:149	Iron oxide nanoparticles-incorporated carrageenan (CAR)/PHEMA composites	78:149	Iron oxide nanoparticles-incorporated carrageenan (CAR)/PHEMA composites of interpenetrating network (IPN) type	78:188	Iron oxide nanoparticles-incorporated carrageenan (CAR)/PHEMA composites of interpenetrating network (IPN) type were successfully prepared by in situ ferrite synthesis in the polymer network.
29050574	1	24	theme	carrageenan	116:126	arg1	type					185:188	interpenetrating network (IPN) type	154:188	interpenetrating network (IPN) type	154:188	Iron oxide nanoparticles-incorporated carrageenan (CAR)/PHEMA composites of interpenetrating network (IPN) type were successfully prepared by in situ ferrite synthesis in the polymer network.
29050574	4	25	theme	property	679:686	arg1	evaluation					652:661	evaluation	652:661	evaluation of the magnetic property of the inorganic-hybridized IPN composites	652:729	SQUID magnetometry and X-ray diffractometry were conducted for evaluation of the magnetic property of the inorganic-hybridized IPN composites.
29050574	2	26	theme	CAR	453:455	arg1	gels					457:460	CAR gels	453:460	CAR gels	453:460	The IPN structure was constructed at CAR/PHEMA compositions of 15/85 and 40/60 (wt/wt) by polymerization and cross-linking of 2-hydroxyethylmethacrylate as an impregnating solvent of CAR gels.
29050574	6	27	theme	10-15emu	1013:1020	arg1	sample					1025:1030	10-15emu (g sample)-1	1013:1033	10-15emu (g sample)-1	1013:1033	Specifically, the 40/60 CAR/PHEMA IPN imparted a practically passable value (10-15emu (g sample)-1) of saturation magnetization.
29050574	6	27	theme	10-15emu	1013:1020	arg1	value					1006:1010	a practically passable value	983:1010	a practically passable value (10-15emu (g sample)-1) of saturation magnetization	983:1062	Specifically, the 40/60 CAR/PHEMA IPN imparted a practically passable value (10-15emu (g sample)-1) of saturation magnetization.
29050574	2	28	theme	CAR/PHEMA	307:315	arg1	compositions					317:328	CAR/PHEMA compositions	307:328	CAR/PHEMA compositions of 15/85 and 40/60 (wt/wt)	307:355	The IPN structure was constructed at CAR/PHEMA compositions of 15/85 and 40/60 (wt/wt) by polymerization and cross-linking of 2-hydroxyethylmethacrylate as an impregnating solvent of CAR gels.
29050574	5	29	with	particles	742:750	arg1	sizes					765:769	10-30nm sizes	757:769	10-30nm sizes	757:769	Magnetite particles with 10-30nm sizes were distributed inside the IPNs treated with the repeatable ferrite synthesis; thereby, the hybrids displayed a superparamagnetic character at ambient temperature.
29050574	3	30	theme	gelatinous	571:580	arg1	state					582:586	their gelatinous state	565:586	their gelatinous state	565:586	As a result of this IPN construction, the composites were firm and showed a good shape-retentivity in their gelatinous state.
29050574	2	31	theme	gels	457:460	arg1	solvent					442:448	an impregnating solvent	426:448	an impregnating solvent of CAR gels	426:460	The IPN structure was constructed at CAR/PHEMA compositions of 15/85 and 40/60 (wt/wt) by polymerization and cross-linking of 2-hydroxyethylmethacrylate as an impregnating solvent of CAR gels.
29050574	4	32	theme	IPN	716:718	arg1	composites					720:729	the inorganic-hybridized IPN composites	691:729	the inorganic-hybridized IPN composites	691:729	SQUID magnetometry and X-ray diffractometry were conducted for evaluation of the magnetic property of the inorganic-hybridized IPN composites.
29050574	6	33	theme	CAR/PHEMA	960:968	arg1	IPN					970:972	the 40/60 CAR/PHEMA IPN	950:972	the 40/60 CAR/PHEMA IPN	950:972	Specifically, the 40/60 CAR/PHEMA IPN imparted a practically passable value (10-15emu (g sample)-1) of saturation magnetization.
29050574	2	34	theme	impregnating	429:440	arg1	solvent					442:448	an impregnating solvent	426:448	an impregnating solvent of CAR gels	426:460	The IPN structure was constructed at CAR/PHEMA compositions of 15/85 and 40/60 (wt/wt) by polymerization and cross-linking of 2-hydroxyethylmethacrylate as an impregnating solvent of CAR gels.
29050574	2	35	theme	40/60	343:347	arg1	compositions					317:328	CAR/PHEMA compositions	307:328	CAR/PHEMA compositions of 15/85 and 40/60 (wt/wt)	307:355	The IPN structure was constructed at CAR/PHEMA compositions of 15/85 and 40/60 (wt/wt) by polymerization and cross-linking of 2-hydroxyethylmethacrylate as an impregnating solvent of CAR gels.
29050574	5	36	theme	ambient	915:921	arg1	temperature					923:933	ambient temperature	915:933	ambient temperature	915:933	Magnetite particles with 10-30nm sizes were distributed inside the IPNs treated with the repeatable ferrite synthesis; thereby, the hybrids displayed a superparamagnetic character at ambient temperature.
29050574	1	37	theme	in	220:221	arg1	synthesis					236:244	in situ ferrite synthesis	220:244	in situ ferrite synthesis in the polymer network	220:267	Iron oxide nanoparticles-incorporated carrageenan (CAR)/PHEMA composites of interpenetrating network (IPN) type were successfully prepared by in situ ferrite synthesis in the polymer network.
29050574	0	38	theme	carrageenan/PHEMA	30:46	arg1	gels					22:25	Superparamagnetic IPN gels	0:25	Superparamagnetic IPN gels of carrageenan/PHEMA	0:46	Superparamagnetic IPN gels of carrageenan/PHEMA excelling in shape retention.
29050574	6	39	theme	passable	997:1004	arg1	sample					1025:1030	10-15emu (g sample)-1	1013:1033	10-15emu (g sample)-1	1013:1033	Specifically, the 40/60 CAR/PHEMA IPN imparted a practically passable value (10-15emu (g sample)-1) of saturation magnetization.
29050574	6	39	theme	passable	997:1004	arg1	value					1006:1010	a practically passable value	983:1010	a practically passable value (10-15emu (g sample)-1) of saturation magnetization	983:1062	Specifically, the 40/60 CAR/PHEMA IPN imparted a practically passable value (10-15emu (g sample)-1) of saturation magnetization.
29050574	0	40	theme	shape	61:65	arg1	retention					67:75	shape retention	61:75	shape retention	61:75	Superparamagnetic IPN gels of carrageenan/PHEMA excelling in shape retention.
29050574	7	41	theme	present	1069:1075	arg1	system					1081:1086	The present IPN system	1065:1086	The present IPN system	1065:1086	The present IPN system offers a potential for application as a biocompatible magnetic material used in hydro-surroundings.
29050574	3	42	theme	construction	487:498	arg1	result					468:473	a result	466:473	a result of this IPN construction	466:498	As a result of this IPN construction, the composites were firm and showed a good shape-retentivity in their gelatinous state.
29050574	5	43	theme	ferrite	832:838	arg1	synthesis					840:848	the repeatable ferrite synthesis	817:848	the repeatable ferrite synthesis	817:848	Magnetite particles with 10-30nm sizes were distributed inside the IPNs treated with the repeatable ferrite synthesis; thereby, the hybrids displayed a superparamagnetic character at ambient temperature.
29050574	7	44	theme	IPN	1077:1079	arg1	system					1081:1086	The present IPN system	1065:1086	The present IPN system	1065:1086	The present IPN system offers a potential for application as a biocompatible magnetic material used in hydro-surroundings.
29050574	1	45	theme	/PHEMA	133:138	arg1	composites					140:149	Iron oxide nanoparticles-incorporated carrageenan (CAR)/PHEMA composites	78:149	Iron oxide nanoparticles-incorporated carrageenan (CAR)/PHEMA composites of interpenetrating network (IPN) type	78:188	Iron oxide nanoparticles-incorporated carrageenan (CAR)/PHEMA composites of interpenetrating network (IPN) type were successfully prepared by in situ ferrite synthesis in the polymer network.
29050574	1	45	theme	/PHEMA	133:138	arg1	type					185:188	interpenetrating network (IPN) type	154:188	interpenetrating network (IPN) type	154:188	Iron oxide nanoparticles-incorporated carrageenan (CAR)/PHEMA composites of interpenetrating network (IPN) type were successfully prepared by in situ ferrite synthesis in the polymer network.
29050574	1	46	dep	in	220:221	arg1	situ					223:226	situ	223:226	situ	223:226	Iron oxide nanoparticles-incorporated carrageenan (CAR)/PHEMA composites of interpenetrating network (IPN) type were successfully prepared by in situ ferrite synthesis in the polymer network.
29050574	1	47	theme	ferrite	228:234	arg1	synthesis					236:244	in situ ferrite synthesis	220:244	in situ ferrite synthesis in the polymer network	220:267	Iron oxide nanoparticles-incorporated carrageenan (CAR)/PHEMA composites of interpenetrating network (IPN) type were successfully prepared by in situ ferrite synthesis in the polymer network.
29050574	1	48	from	synthesis	236:244	arg1	network					261:267	the polymer network	249:267	the polymer network	249:267	Iron oxide nanoparticles-incorporated carrageenan (CAR)/PHEMA composites of interpenetrating network (IPN) type were successfully prepared by in situ ferrite synthesis in the polymer network.
29050574	2	49	theme	15/85	333:337	arg1	compositions					317:328	CAR/PHEMA compositions	307:328	CAR/PHEMA compositions of 15/85 and 40/60 (wt/wt)	307:355	The IPN structure was constructed at CAR/PHEMA compositions of 15/85 and 40/60 (wt/wt) by polymerization and cross-linking of 2-hydroxyethylmethacrylate as an impregnating solvent of CAR gels.
29050574	6	50	theme	saturation	1039:1048	arg1	magnetization					1050:1062	saturation magnetization	1039:1062	saturation magnetization	1039:1062	Specifically, the 40/60 CAR/PHEMA IPN imparted a practically passable value (10-15emu (g sample)-1) of saturation magnetization.
29050574	1	51	theme	interpenetrating	154:169	arg1	type					185:188	interpenetrating network (IPN) type	154:188	interpenetrating network (IPN) type	154:188	Iron oxide nanoparticles-incorporated carrageenan (CAR)/PHEMA composites of interpenetrating network (IPN) type were successfully prepared by in situ ferrite synthesis in the polymer network.
29050574	2	52	theme	IPN	274:276	arg1	structure					278:286	The IPN structure	270:286	The IPN structure	270:286	The IPN structure was constructed at CAR/PHEMA compositions of 15/85 and 40/60 (wt/wt) by polymerization and cross-linking of 2-hydroxyethylmethacrylate as an impregnating solvent of CAR gels.
29050574	1	53	theme	network	171:177	arg1	type					185:188	interpenetrating network (IPN) type	154:188	interpenetrating network (IPN) type	154:188	Iron oxide nanoparticles-incorporated carrageenan (CAR)/PHEMA composites of interpenetrating network (IPN) type were successfully prepared by in situ ferrite synthesis in the polymer network.
27161951	0	0	theme	fatty	83:87	arg1	acid					89:92	fecal fatty acid	77:92	fecal fatty acid	77:92	Black tea polyphenols and polysaccharides improve body composition, increase fecal fatty acid, and regulate fat metabolism in high-fat diet-induced obese rats.
27161951	4	1	theme	fecal	652:656	arg1	content					669:675	the fecal fatty acid content	648:675	the fecal fatty acid content	648:675	The BT polyphenols and polysaccharides reduced the body weight, Lee's index, visceral fat weight, and fat cell size but improved the biochemical profile and increased the fecal fatty acid content, thereby preventing high-fat diet-induced obesity.
27161951	0	2	theme	fecal	77:81	arg1	acid					89:92	fecal fatty acid	77:92	fecal fatty acid	77:92	Black tea polyphenols and polysaccharides improve body composition, increase fecal fatty acid, and regulate fat metabolism in high-fat diet-induced obese rats.
27161951	3	3	from	mechanism	392:400	arg1	rats					475:478	male Sprague-Dawley rats	455:478	male Sprague-Dawley rats	455:478	This study investigated the anti-obesity effect and the mechanism of black tea (BT) polyphenols and polysaccharides in male Sprague-Dawley rats.
27161951	5	4	theme	metabolic	867:875	arg1	degradation					955:965	fatty acid degradation	944:965	fatty acid degradation	944:965	A gene expression profile array was used to screen eight upregulated and five downregulated differentially expressed genes that affect fat metabolic pathways, such as glycerolipid and glycerophospholipid metabolism, fatty acid degradation, glycolysis and gluconeogenesis, bile and pancreatic secretion, the insulin signaling pathway, and steroid hormone secretion.
27161951	5	4	theme	metabolic	867:875	arg1	secretion					1082:1090	steroid hormone secretion	1066:1090	steroid hormone secretion	1066:1090	A gene expression profile array was used to screen eight upregulated and five downregulated differentially expressed genes that affect fat metabolic pathways, such as glycerolipid and glycerophospholipid metabolism, fatty acid degradation, glycolysis and gluconeogenesis, bile and pancreatic secretion, the insulin signaling pathway, and steroid hormone secretion.
27161951	5	4	theme	metabolic	867:875	arg1	bile					1000:1003	bile	1000:1003	bile	1000:1003	A gene expression profile array was used to screen eight upregulated and five downregulated differentially expressed genes that affect fat metabolic pathways, such as glycerolipid and glycerophospholipid metabolism, fatty acid degradation, glycolysis and gluconeogenesis, bile and pancreatic secretion, the insulin signaling pathway, and steroid hormone secretion.
27161951	5	4	theme	metabolic	867:875	arg1	pathways					877:884	fat metabolic pathways	863:884	fat metabolic pathways	863:884	A gene expression profile array was used to screen eight upregulated and five downregulated differentially expressed genes that affect fat metabolic pathways, such as glycerolipid and glycerophospholipid metabolism, fatty acid degradation, glycolysis and gluconeogenesis, bile and pancreatic secretion, the insulin signaling pathway, and steroid hormone secretion.
27161951	5	4	theme	metabolic	867:875	arg1	pathway					1053:1059	the insulin signaling pathway	1031:1059	the insulin signaling pathway	1031:1059	A gene expression profile array was used to screen eight upregulated and five downregulated differentially expressed genes that affect fat metabolic pathways, such as glycerolipid and glycerophospholipid metabolism, fatty acid degradation, glycolysis and gluconeogenesis, bile and pancreatic secretion, the insulin signaling pathway, and steroid hormone secretion.
27161951	5	4	theme	metabolic	867:875	arg1	metabolism					932:941	glycerolipid and glycerophospholipid metabolism	895:941	metabolism	932:941	A gene expression profile array was used to screen eight upregulated and five downregulated differentially expressed genes that affect fat metabolic pathways, such as glycerolipid and glycerophospholipid metabolism, fatty acid degradation, glycolysis and gluconeogenesis, bile and pancreatic secretion, the insulin signaling pathway, and steroid hormone secretion.
27161951	5	4	theme	metabolic	867:875	arg1	glycolysis					968:977	glycolysis	968:977	glycolysis	968:977	A gene expression profile array was used to screen eight upregulated and five downregulated differentially expressed genes that affect fat metabolic pathways, such as glycerolipid and glycerophospholipid metabolism, fatty acid degradation, glycolysis and gluconeogenesis, bile and pancreatic secretion, the insulin signaling pathway, and steroid hormone secretion.
27161951	4	5	theme	BT	485:486	arg1	polyphenols					488:498	The BT polyphenols	481:498	The BT polyphenols	481:498	The BT polyphenols and polysaccharides reduced the body weight, Lee's index, visceral fat weight, and fat cell size but improved the biochemical profile and increased the fecal fatty acid content, thereby preventing high-fat diet-induced obesity.
27161951	5	6	theme	fat	863:865	arg1	degradation					955:965	fatty acid degradation	944:965	fatty acid degradation	944:965	A gene expression profile array was used to screen eight upregulated and five downregulated differentially expressed genes that affect fat metabolic pathways, such as glycerolipid and glycerophospholipid metabolism, fatty acid degradation, glycolysis and gluconeogenesis, bile and pancreatic secretion, the insulin signaling pathway, and steroid hormone secretion.
27161951	5	6	theme	fat	863:865	arg1	secretion					1082:1090	steroid hormone secretion	1066:1090	steroid hormone secretion	1066:1090	A gene expression profile array was used to screen eight upregulated and five downregulated differentially expressed genes that affect fat metabolic pathways, such as glycerolipid and glycerophospholipid metabolism, fatty acid degradation, glycolysis and gluconeogenesis, bile and pancreatic secretion, the insulin signaling pathway, and steroid hormone secretion.
27161951	5	6	theme	fat	863:865	arg1	bile					1000:1003	bile	1000:1003	bile	1000:1003	A gene expression profile array was used to screen eight upregulated and five downregulated differentially expressed genes that affect fat metabolic pathways, such as glycerolipid and glycerophospholipid metabolism, fatty acid degradation, glycolysis and gluconeogenesis, bile and pancreatic secretion, the insulin signaling pathway, and steroid hormone secretion.
27161951	5	6	theme	fat	863:865	arg1	pathways					877:884	fat metabolic pathways	863:884	fat metabolic pathways	863:884	A gene expression profile array was used to screen eight upregulated and five downregulated differentially expressed genes that affect fat metabolic pathways, such as glycerolipid and glycerophospholipid metabolism, fatty acid degradation, glycolysis and gluconeogenesis, bile and pancreatic secretion, the insulin signaling pathway, and steroid hormone secretion.
27161951	5	6	theme	fat	863:865	arg1	pathway					1053:1059	the insulin signaling pathway	1031:1059	the insulin signaling pathway	1031:1059	A gene expression profile array was used to screen eight upregulated and five downregulated differentially expressed genes that affect fat metabolic pathways, such as glycerolipid and glycerophospholipid metabolism, fatty acid degradation, glycolysis and gluconeogenesis, bile and pancreatic secretion, the insulin signaling pathway, and steroid hormone secretion.
27161951	5	6	theme	fat	863:865	arg1	metabolism					932:941	glycerolipid and glycerophospholipid metabolism	895:941	metabolism	932:941	A gene expression profile array was used to screen eight upregulated and five downregulated differentially expressed genes that affect fat metabolic pathways, such as glycerolipid and glycerophospholipid metabolism, fatty acid degradation, glycolysis and gluconeogenesis, bile and pancreatic secretion, the insulin signaling pathway, and steroid hormone secretion.
27161951	5	6	theme	fat	863:865	arg1	glycolysis					968:977	glycolysis	968:977	glycolysis	968:977	A gene expression profile array was used to screen eight upregulated and five downregulated differentially expressed genes that affect fat metabolic pathways, such as glycerolipid and glycerophospholipid metabolism, fatty acid degradation, glycolysis and gluconeogenesis, bile and pancreatic secretion, the insulin signaling pathway, and steroid hormone secretion.
27161951	1	7	theme	global	233:238	arg1	problem					247:253	a global health problem	231:253	a global health problem	231:253	With the current changes in diet and living habits, obesity has become a global health problem.
27161951	5	8	theme	hormone	1074:1080	arg1	secretion					1082:1090	steroid hormone secretion	1066:1090	steroid hormone secretion	1066:1090	A gene expression profile array was used to screen eight upregulated and five downregulated differentially expressed genes that affect fat metabolic pathways, such as glycerolipid and glycerophospholipid metabolism, fatty acid degradation, glycolysis and gluconeogenesis, bile and pancreatic secretion, the insulin signaling pathway, and steroid hormone secretion.
27161951	6	9	theme	fat	1177:1179	arg1	formation					1147:1155	formation	1147:1155	formation	1147:1155	The BT polyphenols and polysaccharides suppressed the formation and accumulation of fat and promoted its decomposition to prevent obesity.
27161951	6	9	theme	fat	1177:1179	arg1	accumulation					1161:1172	accumulation	1161:1172	accumulation	1161:1172	The BT polyphenols and polysaccharides suppressed the formation and accumulation of fat and promoted its decomposition to prevent obesity.
27161951	5	10	theme	glycerolipid	895:906	arg1	metabolism					932:941	glycerolipid and glycerophospholipid metabolism	895:941	metabolism	932:941	A gene expression profile array was used to screen eight upregulated and five downregulated differentially expressed genes that affect fat metabolic pathways, such as glycerolipid and glycerophospholipid metabolism, fatty acid degradation, glycolysis and gluconeogenesis, bile and pancreatic secretion, the insulin signaling pathway, and steroid hormone secretion.
27161951	5	11	theme	acid	950:953	arg1	degradation					955:965	fatty acid degradation	944:965	fatty acid degradation	944:965	A gene expression profile array was used to screen eight upregulated and five downregulated differentially expressed genes that affect fat metabolic pathways, such as glycerolipid and glycerophospholipid metabolism, fatty acid degradation, glycolysis and gluconeogenesis, bile and pancreatic secretion, the insulin signaling pathway, and steroid hormone secretion.
27161951	1	12	theme	health	240:245	arg1	problem					247:253	a global health problem	231:253	a global health problem	231:253	With the current changes in diet and living habits, obesity has become a global health problem.
27161951	5	13	theme	gene	730:733	arg1	profile					746:752	A gene expression profile	728:752	A gene expression profile array	728:758	A gene expression profile array was used to screen eight upregulated and five downregulated differentially expressed genes that affect fat metabolic pathways, such as glycerolipid and glycerophospholipid metabolism, fatty acid degradation, glycolysis and gluconeogenesis, bile and pancreatic secretion, the insulin signaling pathway, and steroid hormone secretion.
27161951	3	14	theme	Sprague-Dawley	460:473	arg1	rats					475:478	male Sprague-Dawley rats	455:478	male Sprague-Dawley rats	455:478	This study investigated the anti-obesity effect and the mechanism of black tea (BT) polyphenols and polysaccharides in male Sprague-Dawley rats.
27161951	4	15	theme	fat	583:585	arg1	size					592:595	fat cell size	583:595	fat cell size	583:595	The BT polyphenols and polysaccharides reduced the body weight, Lee's index, visceral fat weight, and fat cell size but improved the biochemical profile and increased the fecal fatty acid content, thereby preventing high-fat diet-induced obesity.
27161951	3	16	theme	black	405:409	arg1	polyphenols					420:430	black tea (BT) polyphenols	405:430	black tea (BT) polyphenols	405:430	This study investigated the anti-obesity effect and the mechanism of black tea (BT) polyphenols and polysaccharides in male Sprague-Dawley rats.
27161951	3	17	theme	BT	416:417	arg1	polyphenols					420:430	black tea (BT) polyphenols	405:430	black tea (BT) polyphenols	405:430	This study investigated the anti-obesity effect and the mechanism of black tea (BT) polyphenols and polysaccharides in male Sprague-Dawley rats.
27161951	0	18	theme	tea	6:8	arg1	polyphenols					10:20	Black tea polyphenols	0:20	Black tea polyphenols	0:20	Black tea polyphenols and polysaccharides improve body composition, increase fecal fatty acid, and regulate fat metabolism in high-fat diet-induced obese rats.
27161951	3	19	from	effect	377:382	arg1	rats					475:478	male Sprague-Dawley rats	455:478	male Sprague-Dawley rats	455:478	This study investigated the anti-obesity effect and the mechanism of black tea (BT) polyphenols and polysaccharides in male Sprague-Dawley rats.
27161951	1	20	theme	current	169:175	arg1	changes					177:183	the current changes	165:183	the current changes in diet and living habits	165:209	With the current changes in diet and living habits, obesity has become a global health problem.
27161951	0	21	theme	Black	0:4	arg1	polyphenols					10:20	Black tea polyphenols	0:20	Black tea polyphenols	0:20	Black tea polyphenols and polysaccharides improve body composition, increase fecal fatty acid, and regulate fat metabolism in high-fat diet-induced obese rats.
27161951	5	22	theme	signaling	1043:1051	arg1	pathway					1053:1059	the insulin signaling pathway	1031:1059	the insulin signaling pathway	1031:1059	A gene expression profile array was used to screen eight upregulated and five downregulated differentially expressed genes that affect fat metabolic pathways, such as glycerolipid and glycerophospholipid metabolism, fatty acid degradation, glycolysis and gluconeogenesis, bile and pancreatic secretion, the insulin signaling pathway, and steroid hormone secretion.
27161951	5	23	theme	glycerophospholipid	912:930	arg1	metabolism					932:941	glycerolipid and glycerophospholipid metabolism	895:941	metabolism	932:941	A gene expression profile array was used to screen eight upregulated and five downregulated differentially expressed genes that affect fat metabolic pathways, such as glycerolipid and glycerophospholipid metabolism, fatty acid degradation, glycolysis and gluconeogenesis, bile and pancreatic secretion, the insulin signaling pathway, and steroid hormone secretion.
27161951	3	24	theme	tea	411:413	arg1	polyphenols					420:430	black tea (BT) polyphenols	405:430	black tea (BT) polyphenols	405:430	This study investigated the anti-obesity effect and the mechanism of black tea (BT) polyphenols and polysaccharides in male Sprague-Dawley rats.
27161951	4	25	theme	body	532:535	arg1	weight					537:542	the body weight	528:542	the body weight	528:542	The BT polyphenols and polysaccharides reduced the body weight, Lee's index, visceral fat weight, and fat cell size but improved the biochemical profile and increased the fecal fatty acid content, thereby preventing high-fat diet-induced obesity.
27161951	3	26	from	rats	475:478	arg1	effect					377:382	the anti-obesity effect	360:382	the anti-obesity effect	360:382	This study investigated the anti-obesity effect and the mechanism of black tea (BT) polyphenols and polysaccharides in male Sprague-Dawley rats.
27161951	3	26	from	rats	475:478	arg1	mechanism					392:400	the mechanism	388:400	the mechanism of black tea (BT) polyphenols and polysaccharides in male Sprague-Dawley rats	388:478	This study investigated the anti-obesity effect and the mechanism of black tea (BT) polyphenols and polysaccharides in male Sprague-Dawley rats.
27161951	2	27	theme	weight-reducing	266:280	arg1	function					282:289	the weight-reducing function	262:289	the weight-reducing function of tea	262:296	Thus, the weight-reducing function of tea has attracted considerable attention.
27161951	4	28	theme	cell	587:590	arg1	size					592:595	fat cell size	583:595	fat cell size	583:595	The BT polyphenols and polysaccharides reduced the body weight, Lee's index, visceral fat weight, and fat cell size but improved the biochemical profile and increased the fecal fatty acid content, thereby preventing high-fat diet-induced obesity.
27161951	6	29	theme	BT	1097:1098	arg1	polyphenols					1100:1110	The BT polyphenols	1093:1110	The BT polyphenols	1093:1110	The BT polyphenols and polysaccharides suppressed the formation and accumulation of fat and promoted its decomposition to prevent obesity.
27161951	0	30	theme	fat	108:110	arg1	metabolism					112:121	fat metabolism	108:121	fat metabolism in high-fat diet-induced obese rats	108:157	Black tea polyphenols and polysaccharides improve body composition, increase fecal fatty acid, and regulate fat metabolism in high-fat diet-induced obese rats.
27161951	4	31	theme	high-fat	697:704	arg1	obesity					719:725	high-fat diet-induced obesity	697:725	high-fat diet-induced obesity	697:725	The BT polyphenols and polysaccharides reduced the body weight, Lee's index, visceral fat weight, and fat cell size but improved the biochemical profile and increased the fecal fatty acid content, thereby preventing high-fat diet-induced obesity.
27161951	3	32	theme	polysaccharides	436:450	arg1	effect					377:382	the anti-obesity effect	360:382	the anti-obesity effect	360:382	This study investigated the anti-obesity effect and the mechanism of black tea (BT) polyphenols and polysaccharides in male Sprague-Dawley rats.
27161951	3	32	theme	polysaccharides	436:450	arg1	mechanism					392:400	the mechanism	388:400	the mechanism of black tea (BT) polyphenols and polysaccharides in male Sprague-Dawley rats	388:478	This study investigated the anti-obesity effect and the mechanism of black tea (BT) polyphenols and polysaccharides in male Sprague-Dawley rats.
27161951	5	33	theme	expression	735:744	arg1	profile					746:752	A gene expression profile	728:752	A gene expression profile array	728:758	A gene expression profile array was used to screen eight upregulated and five downregulated differentially expressed genes that affect fat metabolic pathways, such as glycerolipid and glycerophospholipid metabolism, fatty acid degradation, glycolysis and gluconeogenesis, bile and pancreatic secretion, the insulin signaling pathway, and steroid hormone secretion.
27161951	0	34	theme	high-fat	126:133	arg1	rats					154:157	high-fat diet-induced obese rats	126:157	high-fat diet-induced obese rats	126:157	Black tea polyphenols and polysaccharides improve body composition, increase fecal fatty acid, and regulate fat metabolism in high-fat diet-induced obese rats.
27161951	4	35	theme	biochemical	614:624	arg1	profile					626:632	the biochemical profile	610:632	the biochemical profile	610:632	The BT polyphenols and polysaccharides reduced the body weight, Lee's index, visceral fat weight, and fat cell size but improved the biochemical profile and increased the fecal fatty acid content, thereby preventing high-fat diet-induced obesity.
27161951	5	36	theme	profile	746:752	arg1	array					754:758	A gene expression profile array	728:758	A gene expression profile array	728:758	A gene expression profile array was used to screen eight upregulated and five downregulated differentially expressed genes that affect fat metabolic pathways, such as glycerolipid and glycerophospholipid metabolism, fatty acid degradation, glycolysis and gluconeogenesis, bile and pancreatic secretion, the insulin signaling pathway, and steroid hormone secretion.
27161951	5	37	theme	expressed	835:843	arg1	genes					845:849	five downregulated differentially expressed genes that affect fat metabolic pathways, such as glycerolipid and glycerophospholipid metabolism, fatty acid degradation, glycolysis and gluconeogenesis, bile and pancreatic secretion, the insulin signaling pathway, and steroid hormone secretion	801:1090	five downregulated differentially expressed genes that affect fat metabolic pathways, such as glycerolipid and glycerophospholipid metabolism, fatty acid degradation, glycolysis and gluconeogenesis, bile and pancreatic secretion, the insulin signaling pathway, and steroid hormone secretion	801:1090	A gene expression profile array was used to screen eight upregulated and five downregulated differentially expressed genes that affect fat metabolic pathways, such as glycerolipid and glycerophospholipid metabolism, fatty acid degradation, glycolysis and gluconeogenesis, bile and pancreatic secretion, the insulin signaling pathway, and steroid hormone secretion.
27161951	6	38	dep	formation	1147:1155	arg1	the					1143:1145	the	1143:1145	the	1143:1145	The BT polyphenols and polysaccharides suppressed the formation and accumulation of fat and promoted its decomposition to prevent obesity.
27161951	5	39	theme	downregulated	806:818	arg1	genes					845:849	five downregulated differentially expressed genes that affect fat metabolic pathways, such as glycerolipid and glycerophospholipid metabolism, fatty acid degradation, glycolysis and gluconeogenesis, bile and pancreatic secretion, the insulin signaling pathway, and steroid hormone secretion	801:1090	five downregulated differentially expressed genes that affect fat metabolic pathways, such as glycerolipid and glycerophospholipid metabolism, fatty acid degradation, glycolysis and gluconeogenesis, bile and pancreatic secretion, the insulin signaling pathway, and steroid hormone secretion	801:1090	A gene expression profile array was used to screen eight upregulated and five downregulated differentially expressed genes that affect fat metabolic pathways, such as glycerolipid and glycerophospholipid metabolism, fatty acid degradation, glycolysis and gluconeogenesis, bile and pancreatic secretion, the insulin signaling pathway, and steroid hormone secretion.
27161951	3	40	theme	polyphenols	420:430	arg1	effect					377:382	the anti-obesity effect	360:382	the anti-obesity effect	360:382	This study investigated the anti-obesity effect and the mechanism of black tea (BT) polyphenols and polysaccharides in male Sprague-Dawley rats.
27161951	3	40	theme	polyphenols	420:430	arg1	mechanism					392:400	the mechanism	388:400	the mechanism of black tea (BT) polyphenols and polysaccharides in male Sprague-Dawley rats	388:478	This study investigated the anti-obesity effect and the mechanism of black tea (BT) polyphenols and polysaccharides in male Sprague-Dawley rats.
27161951	0	41	theme	obese	148:152	arg1	rats					154:157	high-fat diet-induced obese rats	126:157	high-fat diet-induced obese rats	126:157	Black tea polyphenols and polysaccharides improve body composition, increase fecal fatty acid, and regulate fat metabolism in high-fat diet-induced obese rats.
27161951	5	42	theme	steroid	1066:1072	arg1	secretion					1082:1090	steroid hormone secretion	1066:1090	steroid hormone secretion	1066:1090	A gene expression profile array was used to screen eight upregulated and five downregulated differentially expressed genes that affect fat metabolic pathways, such as glycerolipid and glycerophospholipid metabolism, fatty acid degradation, glycolysis and gluconeogenesis, bile and pancreatic secretion, the insulin signaling pathway, and steroid hormone secretion.
27161951	5	43	theme	pancreatic	1009:1018	arg1	secretion					1020:1028	pancreatic secretion	1009:1028	pancreatic secretion	1009:1028	A gene expression profile array was used to screen eight upregulated and five downregulated differentially expressed genes that affect fat metabolic pathways, such as glycerolipid and glycerophospholipid metabolism, fatty acid degradation, glycolysis and gluconeogenesis, bile and pancreatic secretion, the insulin signaling pathway, and steroid hormone secretion.
27161951	3	44	from	polyphenols	420:430	arg1	rats					475:478	male Sprague-Dawley rats	455:478	male Sprague-Dawley rats	455:478	This study investigated the anti-obesity effect and the mechanism of black tea (BT) polyphenols and polysaccharides in male Sprague-Dawley rats.
27161951	2	45	theme	considerable	312:323	arg1	attention					325:333	considerable attention	312:333	considerable attention	312:333	Thus, the weight-reducing function of tea has attracted considerable attention.
27161951	0	46	theme	body	50:53	arg1	composition					55:65	body composition	50:65	body composition	50:65	Black tea polyphenols and polysaccharides improve body composition, increase fecal fatty acid, and regulate fat metabolism in high-fat diet-induced obese rats.
27161951	0	47	theme	diet-induced	135:146	arg1	rats					154:157	high-fat diet-induced obese rats	126:157	high-fat diet-induced obese rats	126:157	Black tea polyphenols and polysaccharides improve body composition, increase fecal fatty acid, and regulate fat metabolism in high-fat diet-induced obese rats.
27161951	4	48	theme	fatty	658:662	arg1	content					669:675	the fecal fatty acid content	648:675	the fecal fatty acid content	648:675	The BT polyphenols and polysaccharides reduced the body weight, Lee's index, visceral fat weight, and fat cell size but improved the biochemical profile and increased the fecal fatty acid content, thereby preventing high-fat diet-induced obesity.
27161951	4	49	theme	acid	664:667	arg1	content					669:675	the fecal fatty acid content	648:675	the fecal fatty acid content	648:675	The BT polyphenols and polysaccharides reduced the body weight, Lee's index, visceral fat weight, and fat cell size but improved the biochemical profile and increased the fecal fatty acid content, thereby preventing high-fat diet-induced obesity.
27161951	3	50	from	polysaccharides	436:450	arg1	rats					475:478	male Sprague-Dawley rats	455:478	male Sprague-Dawley rats	455:478	This study investigated the anti-obesity effect and the mechanism of black tea (BT) polyphenols and polysaccharides in male Sprague-Dawley rats.
27161951	4	51	theme	diet-induced	706:717	arg1	obesity					719:725	high-fat diet-induced obesity	697:725	high-fat diet-induced obesity	697:725	The BT polyphenols and polysaccharides reduced the body weight, Lee's index, visceral fat weight, and fat cell size but improved the biochemical profile and increased the fecal fatty acid content, thereby preventing high-fat diet-induced obesity.
27161951	5	52	used	used	764:767	arg2	array					754:758	A gene expression profile array	728:758	A gene expression profile array	728:758	A gene expression profile array was used to screen eight upregulated and five downregulated differentially expressed genes that affect fat metabolic pathways, such as glycerolipid and glycerophospholipid metabolism, fatty acid degradation, glycolysis and gluconeogenesis, bile and pancreatic secretion, the insulin signaling pathway, and steroid hormone secretion.
27161951	5	53	theme	insulin	1035:1041	arg1	pathway					1053:1059	the insulin signaling pathway	1031:1059	the insulin signaling pathway	1031:1059	A gene expression profile array was used to screen eight upregulated and five downregulated differentially expressed genes that affect fat metabolic pathways, such as glycerolipid and glycerophospholipid metabolism, fatty acid degradation, glycolysis and gluconeogenesis, bile and pancreatic secretion, the insulin signaling pathway, and steroid hormone secretion.
27161951	5	54	theme	fatty	944:948	arg1	degradation					955:965	fatty acid degradation	944:965	fatty acid degradation	944:965	A gene expression profile array was used to screen eight upregulated and five downregulated differentially expressed genes that affect fat metabolic pathways, such as glycerolipid and glycerophospholipid metabolism, fatty acid degradation, glycolysis and gluconeogenesis, bile and pancreatic secretion, the insulin signaling pathway, and steroid hormone secretion.
27161951	2	55	theme	tea	294:296	arg1	function					282:289	the weight-reducing function	262:289	the weight-reducing function of tea	262:296	Thus, the weight-reducing function of tea has attracted considerable attention.
27161951	4	56	theme	fat	567:569	arg1	weight					571:576	visceral fat weight	558:576	visceral fat weight	558:576	The BT polyphenols and polysaccharides reduced the body weight, Lee's index, visceral fat weight, and fat cell size but improved the biochemical profile and increased the fecal fatty acid content, thereby preventing high-fat diet-induced obesity.
27161951	1	57	theme	living	197:202	arg1	habits					204:209	living habits	197:209	living habits	197:209	With the current changes in diet and living habits, obesity has become a global health problem.
27161951	1	58	from	changes	177:183	arg1	diet					188:191	diet	188:191	diet	188:191	With the current changes in diet and living habits, obesity has become a global health problem.
27161951	1	58	from	changes	177:183	arg1	habits					204:209	living habits	197:209	living habits	197:209	With the current changes in diet and living habits, obesity has become a global health problem.
27161951	3	59	theme	male	455:458	arg1	rats					475:478	male Sprague-Dawley rats	455:478	male Sprague-Dawley rats	455:478	This study investigated the anti-obesity effect and the mechanism of black tea (BT) polyphenols and polysaccharides in male Sprague-Dawley rats.
27161951	0	60	from	metabolism	112:121	arg1	rats					154:157	high-fat diet-induced obese rats	126:157	high-fat diet-induced obese rats	126:157	Black tea polyphenols and polysaccharides improve body composition, increase fecal fatty acid, and regulate fat metabolism in high-fat diet-induced obese rats.
27161951	4	61	theme	visceral	558:565	arg1	weight					571:576	visceral fat weight	558:576	visceral fat weight	558:576	The BT polyphenols and polysaccharides reduced the body weight, Lee's index, visceral fat weight, and fat cell size but improved the biochemical profile and increased the fecal fatty acid content, thereby preventing high-fat diet-induced obesity.
27161951	3	62	theme	anti-obesity	364:375	arg1	effect					377:382	the anti-obesity effect	360:382	the anti-obesity effect	360:382	This study investigated the anti-obesity effect and the mechanism of black tea (BT) polyphenols and polysaccharides in male Sprague-Dawley rats.
25241532	6	0	theme	grafting	727:734	arg1	effect					736:741	The grafting effect	723:741	The grafting effect of this complex on the titanium surface	723:781	The grafting effect of this complex on the titanium surface was detected by EB staining.
25241532	1	1	dep	OBJECTIVE	70:78	arg1	conducted					94:102	conducted	94:102	is conducted to explore new methods to perform surface biomodification of titanium implants and improve osteogenic efficiency	91:215	OBJECTIVE This study is conducted to explore new methods to perform surface biomodification of titanium implants and improve osteogenic efficiency.
25241532	11	2	used	used	1174:1177	arg2	carrier					1225:1231	a titanium implant surface plasmid package carrier	1182:1231	a titanium implant surface plasmid package carrier of pDNA	1182:1239	CONCLUSION RGD peptide-modified CS can be used as a titanium implant surface plasmid package carrier of pDNA.
25241532	11	2	used	used	1174:1177	arg2	CS					1164:1165	CONCLUSION RGD peptide-modified CS	1132:1165	CONCLUSION RGD peptide-modified CS	1132:1165	CONCLUSION RGD peptide-modified CS can be used as a titanium implant surface plasmid package carrier of pDNA.
25241532	4	3	theme	structural	507:516	arg1	characteristics					518:532	The chemical structural characteristics	494:532	The chemical structural characteristics of RGD-CS	494:542	The chemical structural characteristics of RGD-CS were evaluated using an infrared spectrometer and an elemental analyzer.
25241532	1	4	theme	surface	138:144	arg1	biomodification					146:160	surface biomodification	138:160	surface biomodification of titanium implants	138:181	OBJECTIVE This study is conducted to explore new methods to perform surface biomodification of titanium implants and improve osteogenic efficiency.
25241532	7	5	theme	amide	859:863	arg1	bond					865:868	an amide bond	856:868	an amide bond	856:868	RESULTS CS and RGD peptides were coupled by an amide bond.
25241532	4	6	theme	chemical	498:505	arg1	characteristics					518:532	The chemical structural characteristics	494:532	The chemical structural characteristics of RGD-CS	494:542	The chemical structural characteristics of RGD-CS were evaluated using an infrared spectrometer and an elemental analyzer.
25241532	4	7	theme	elemental	597:605	arg1	analyzer					607:614	an elemental analyzer	594:614	an elemental analyzer	594:614	The chemical structural characteristics of RGD-CS were evaluated using an infrared spectrometer and an elemental analyzer.
25241532	5	8	theme	gel	664:666	arg1	electrophoresis					668:682	gel electrophoresis	664:682	gel electrophoresis combined with atomic force microscopy	664:720	The shape of this complex was then assessed by gel electrophoresis combined with atomic force microscopy.
25241532	3	9	theme	RGD-CS/pDNA	315:325	arg1	complex					327:333	An RGD-CS/pDNA complex	312:333	An RGD-CS/pDNA complex	312:333	An RGD-CS/pDNA complex was subsequently prepared using a complex coacervation method and grafted on a pure titanium surface after physical and biochemical treatments were performed.
25241532	6	10	theme	EB	799:800	arg1	staining					802:809	EB staining	799:809	EB staining	799:809	The grafting effect of this complex on the titanium surface was detected by EB staining.
25241532	11	11	theme	peptide-modified	1147:1162	arg1	CS					1164:1165	CONCLUSION RGD peptide-modified CS	1132:1165	CONCLUSION RGD peptide-modified CS	1132:1165	CONCLUSION RGD peptide-modified CS can be used as a titanium implant surface plasmid package carrier of pDNA.
25241532	11	11	theme	peptide-modified	1147:1162	arg1	carrier					1225:1231	a titanium implant surface plasmid package carrier	1182:1231	a titanium implant surface plasmid package carrier of pDNA	1182:1239	CONCLUSION RGD peptide-modified CS can be used as a titanium implant surface plasmid package carrier of pDNA.
25241532	9	12	theme	complex	1005:1011	arg1	spherical					1024:1032	spherical	1024:1032	spherical	1024:1032	Atomic force microscopy results showed that the morphology of this complex was mainly spherical.
25241532	9	12	theme	complex	1005:1011	arg1	morphology					986:995	the morphology	982:995	the morphology of this complex	982:1011	Atomic force microscopy results showed that the morphology of this complex was mainly spherical.
25241532	3	13	theme	pure	414:417	arg1	surface					428:434	a pure titanium surface	412:434	a pure titanium surface	412:434	An RGD-CS/pDNA complex was subsequently prepared using a complex coacervation method and grafted on a pure titanium surface after physical and biochemical treatments were performed.
25241532	9	14	theme	force	945:949	arg1	microscopy					951:960	Atomic force microscopy	938:960	Atomic force microscopy results	938:968	Atomic force microscopy results showed that the morphology of this complex was mainly spherical.
25241532	3	15	theme	titanium	419:426	arg1	surface					428:434	a pure titanium surface	412:434	a pure titanium surface	412:434	An RGD-CS/pDNA complex was subsequently prepared using a complex coacervation method and grafted on a pure titanium surface after physical and biochemical treatments were performed.
25241532	9	16	theme	Atomic	938:943	arg1	microscopy					951:960	Atomic force microscopy	938:960	Atomic force microscopy results	938:968	Atomic force microscopy results showed that the morphology of this complex was mainly spherical.
25241532	5	17	theme	complex	635:641	arg1	shape					621:625	The shape	617:625	The shape of this complex	617:641	The shape of this complex was then assessed by gel electrophoresis combined with atomic force microscopy.
25241532	1	18	theme	titanium	165:172	arg1	implants					174:181	titanium implants	165:181	titanium implants	165:181	OBJECTIVE This study is conducted to explore new methods to perform surface biomodification of titanium implants and improve osteogenic efficiency.
25241532	3	19	theme	complex	369:375	arg1	method					390:395	a complex coacervation method	367:395	a complex coacervation method	367:395	An RGD-CS/pDNA complex was subsequently prepared using a complex coacervation method and grafted on a pure titanium surface after physical and biochemical treatments were performed.
25241532	10	20	theme	titanium	1116:1123	arg1	plate					1125:1129	the titanium plate	1112:1129	the titanium plate	1112:1129	EB staining experiments showed that this complex was successfully grafted on the titanium plate.
25241532	10	21	theme	staining	1038:1045	arg1	experiments					1047:1057	EB staining experiments	1035:1057	EB staining experiments	1035:1057	EB staining experiments showed that this complex was successfully grafted on the titanium plate.
25241532	11	22	theme	titanium	1184:1191	arg1	CS					1164:1165	CONCLUSION RGD peptide-modified CS	1132:1165	CONCLUSION RGD peptide-modified CS	1132:1165	CONCLUSION RGD peptide-modified CS can be used as a titanium implant surface plasmid package carrier of pDNA.
25241532	11	22	theme	titanium	1184:1191	arg1	carrier					1225:1231	a titanium implant surface plasmid package carrier	1182:1231	a titanium implant surface plasmid package carrier of pDNA	1182:1239	CONCLUSION RGD peptide-modified CS can be used as a titanium implant surface plasmid package carrier of pDNA.
25241532	7	23	theme	RGD	827:829	arg1	peptides					831:838	RGD peptides	827:838	RGD peptides	827:838	RESULTS CS and RGD peptides were coupled by an amide bond.
25241532	1	24	theme	implants	174:181	arg1	biomodification					146:160	surface biomodification	138:160	surface biomodification of titanium implants	138:181	OBJECTIVE This study is conducted to explore new methods to perform surface biomodification of titanium implants and improve osteogenic efficiency.
25241532	9	25	theme	microscopy	951:960	arg1	results					962:968	Atomic force microscopy results	938:968	Atomic force microscopy results	938:968	Atomic force microscopy results showed that the morphology of this complex was mainly spherical.
25241532	5	26	theme	force	705:709	arg1	microscopy					711:720	atomic force microscopy	698:720	atomic force microscopy	698:720	The shape of this complex was then assessed by gel electrophoresis combined with atomic force microscopy.
25241532	2	27	theme	acylation	276:284	arg1	reaction					286:293	acylation reaction	276:293	acylation reaction	276:293	METHODS An RGD peptide and chitosan (CS) were combined by acylation reaction, forming RGD-CS.
25241532	8	28	theme	RGD-CS/pDNA	875:885	arg1	complex					887:893	The RGD-CS/pDNA complex	871:893	The RGD-CS/pDNA complex	871:893	The RGD-CS/pDNA complex was completely composited at N/P > or = 2.
25241532	3	29	theme	physical	442:449	arg1	treatments					467:476	physical and biochemical treatments	442:476	physical and biochemical treatments	442:476	An RGD-CS/pDNA complex was subsequently prepared using a complex coacervation method and grafted on a pure titanium surface after physical and biochemical treatments were performed.
25241532	4	30	theme	infrared	568:575	arg1	spectrometer					577:588	an infrared spectrometer	565:588	an infrared spectrometer	565:588	The chemical structural characteristics of RGD-CS were evaluated using an infrared spectrometer and an elemental analyzer.
25241532	11	31	theme	package	1217:1223	arg1	CS					1164:1165	CONCLUSION RGD peptide-modified CS	1132:1165	CONCLUSION RGD peptide-modified CS	1132:1165	CONCLUSION RGD peptide-modified CS can be used as a titanium implant surface plasmid package carrier of pDNA.
25241532	11	31	theme	package	1217:1223	arg1	carrier					1225:1231	a titanium implant surface plasmid package carrier	1182:1231	a titanium implant surface plasmid package carrier of pDNA	1182:1239	CONCLUSION RGD peptide-modified CS can be used as a titanium implant surface plasmid package carrier of pDNA.
25241532	0	32	theme	gene	36:39	arg1	chitosan					22:29	chitosan	22:29	chitosan	22:29	[RGD peptide-modified chitosan as a gene carrier of implant surface].
25241532	0	32	theme	gene	36:39	arg1	carrier					41:47	a gene carrier	34:47	a gene carrier of implant surface	34:66	[RGD peptide-modified chitosan as a gene carrier of implant surface].
25241532	8	33	theme	N/P	924:926	arg1	>					928:928	N/P >	924:928	N/P >	924:928	The RGD-CS/pDNA complex was completely composited at N/P > or = 2.
25241532	11	34	theme	implant	1193:1199	arg1	CS					1164:1165	CONCLUSION RGD peptide-modified CS	1132:1165	CONCLUSION RGD peptide-modified CS	1132:1165	CONCLUSION RGD peptide-modified CS can be used as a titanium implant surface plasmid package carrier of pDNA.
25241532	11	34	theme	implant	1193:1199	arg1	carrier					1225:1231	a titanium implant surface plasmid package carrier	1182:1231	a titanium implant surface plasmid package carrier of pDNA	1182:1239	CONCLUSION RGD peptide-modified CS can be used as a titanium implant surface plasmid package carrier of pDNA.
25241532	2	35	theme	RGD	229:231	arg1	METHODS					218:224	METHODS	218:224	METHODS An RGD peptide and chitosan (CS)	218:257	METHODS An RGD peptide and chitosan (CS) were combined by acylation reaction, forming RGD-CS.
25241532	2	35	theme	RGD	229:231	arg1	peptide					233:239	An RGD peptide	226:239	An RGD peptide	226:239	METHODS An RGD peptide and chitosan (CS) were combined by acylation reaction, forming RGD-CS.
25241532	1	36	theme	osteogenic	195:204	arg1	efficiency					206:215	osteogenic efficiency	195:215	osteogenic efficiency	195:215	OBJECTIVE This study is conducted to explore new methods to perform surface biomodification of titanium implants and improve osteogenic efficiency.
25241532	11	37	theme	surface	1201:1207	arg1	CS					1164:1165	CONCLUSION RGD peptide-modified CS	1132:1165	CONCLUSION RGD peptide-modified CS	1132:1165	CONCLUSION RGD peptide-modified CS can be used as a titanium implant surface plasmid package carrier of pDNA.
25241532	11	37	theme	surface	1201:1207	arg1	carrier					1225:1231	a titanium implant surface plasmid package carrier	1182:1231	a titanium implant surface plasmid package carrier of pDNA	1182:1239	CONCLUSION RGD peptide-modified CS can be used as a titanium implant surface plasmid package carrier of pDNA.
25241532	6	38	theme	titanium	766:773	arg1	surface					775:781	the titanium surface	762:781	the titanium surface	762:781	The grafting effect of this complex on the titanium surface was detected by EB staining.
25241532	3	39	theme	coacervation	377:388	arg1	method					390:395	a complex coacervation method	367:395	a complex coacervation method	367:395	An RGD-CS/pDNA complex was subsequently prepared using a complex coacervation method and grafted on a pure titanium surface after physical and biochemical treatments were performed.
25241532	11	40	theme	pDNA	1236:1239	arg1	CS					1164:1165	CONCLUSION RGD peptide-modified CS	1132:1165	CONCLUSION RGD peptide-modified CS	1132:1165	CONCLUSION RGD peptide-modified CS can be used as a titanium implant surface plasmid package carrier of pDNA.
25241532	11	40	theme	pDNA	1236:1239	arg1	carrier					1225:1231	a titanium implant surface plasmid package carrier	1182:1231	a titanium implant surface plasmid package carrier of pDNA	1182:1239	CONCLUSION RGD peptide-modified CS can be used as a titanium implant surface plasmid package carrier of pDNA.
25241532	7	41	dep	RESULTS	812:818	arg1	CS					820:821	CS	820:821	CS	820:821	RESULTS CS and RGD peptides were coupled by an amide bond.
25241532	7	41	dep	RESULTS	812:818	arg1	peptides					831:838	RGD peptides	827:838	RGD peptides	827:838	RESULTS CS and RGD peptides were coupled by an amide bond.
25241532	11	42	theme	plasmid	1209:1215	arg1	CS					1164:1165	CONCLUSION RGD peptide-modified CS	1132:1165	CONCLUSION RGD peptide-modified CS	1132:1165	CONCLUSION RGD peptide-modified CS can be used as a titanium implant surface plasmid package carrier of pDNA.
25241532	11	42	theme	plasmid	1209:1215	arg1	carrier					1225:1231	a titanium implant surface plasmid package carrier	1182:1231	a titanium implant surface plasmid package carrier of pDNA	1182:1239	CONCLUSION RGD peptide-modified CS can be used as a titanium implant surface plasmid package carrier of pDNA.
25241532	10	43	theme	EB	1035:1036	arg1	experiments					1047:1057	EB staining experiments	1035:1057	EB staining experiments	1035:1057	EB staining experiments showed that this complex was successfully grafted on the titanium plate.
25241532	4	44	theme	RGD-CS	537:542	arg1	characteristics					518:532	The chemical structural characteristics	494:532	The chemical structural characteristics of RGD-CS	494:542	The chemical structural characteristics of RGD-CS were evaluated using an infrared spectrometer and an elemental analyzer.
25241532	6	45	theme	complex	751:757	arg1	effect					736:741	The grafting effect	723:741	The grafting effect of this complex on the titanium surface	723:781	The grafting effect of this complex on the titanium surface was detected by EB staining.
25241532	3	46	theme	biochemical	455:465	arg1	treatments					467:476	physical and biochemical treatments	442:476	physical and biochemical treatments	442:476	An RGD-CS/pDNA complex was subsequently prepared using a complex coacervation method and grafted on a pure titanium surface after physical and biochemical treatments were performed.
25241532	5	47	theme	atomic	698:703	arg1	microscopy					711:720	atomic force microscopy	698:720	atomic force microscopy	698:720	The shape of this complex was then assessed by gel electrophoresis combined with atomic force microscopy.
25241532	1	48	theme	new	115:117	arg1	methods					119:125	new methods	115:125	new methods	115:125	OBJECTIVE This study is conducted to explore new methods to perform surface biomodification of titanium implants and improve osteogenic efficiency.
25241532	8	49	dep	=	933:933	arg1	2					935:935	2	935:935	2	935:935	The RGD-CS/pDNA complex was completely composited at N/P > or = 2.
25241532	0	50	theme	surface	60:66	arg1	chitosan					22:29	chitosan	22:29	chitosan	22:29	[RGD peptide-modified chitosan as a gene carrier of implant surface].
25241532	0	50	theme	surface	60:66	arg1	carrier					41:47	a gene carrier	34:47	a gene carrier of implant surface	34:66	[RGD peptide-modified chitosan as a gene carrier of implant surface].
25241532	2	51	dep	METHODS	218:224	arg1	CS					255:256	CS	255:256	CS	255:256	METHODS An RGD peptide and chitosan (CS) were combined by acylation reaction, forming RGD-CS.
25241532	2	51	dep	METHODS	218:224	arg1	chitosan					245:252	chitosan	245:252	chitosan (CS)	245:257	METHODS An RGD peptide and chitosan (CS) were combined by acylation reaction, forming RGD-CS.
25241532	2	51	dep	METHODS	218:224	arg1	METHODS					218:224	METHODS	218:224	METHODS An RGD peptide and chitosan (CS)	218:257	METHODS An RGD peptide and chitosan (CS) were combined by acylation reaction, forming RGD-CS.
25241532	2	51	dep	METHODS	218:224	arg1	peptide					233:239	An RGD peptide	226:239	An RGD peptide	226:239	METHODS An RGD peptide and chitosan (CS) were combined by acylation reaction, forming RGD-CS.
25241532	6	52	from	effect	736:741	arg1	surface					775:781	the titanium surface	762:781	the titanium surface	762:781	The grafting effect of this complex on the titanium surface was detected by EB staining.
25241532	0	53	theme	implant	52:58	arg1	surface					60:66	implant surface	52:66	implant surface	52:66	[RGD peptide-modified chitosan as a gene carrier of implant surface].
24337112	7	0	theme	GXM	1122:1124	arg1	production					1126:1135	diminished GXM production	1111:1135	diminished GXM production during in vitro growth, macrophage infection, and lung colonization	1111:1203	These findings were correlated with diminished GXM production during in vitro growth, macrophage infection, and lung colonization.
24337112	5	1	theme	lower	839:843	arg1	content					849:855	a lower GXM content	837:855	a lower GXM content	837:855	The apt1Δ mutant, however, produced extracellular vesicles (EVs) with a lower GXM content and different size distribution in comparison with those of WT cells.
24337112	4	2	theme	molecular	723:731	arg1	size					733:736	molecular size	723:736	molecular size	723:736	Mutant and wild-type (WT) cells shared important phenotypic characteristics, including capsule morphology and dimensions, glucuronoxylomannan (GXM) composition, molecular size, and serological properties.
24337112	9	3	theme	animal	1669:1674	arg1	infection					1676:1684	animal infection	1669:1684	animal infection	1669:1684	Taken together, our results indicate that the lack of APT1 caused defects in both GXM synthesis and vesicular export to the extracellular milieu by C. neoformans via processes that are apparently related to the pathogenic mechanisms used by this fungus during animal infection.
24337112	3	4	theme	Apt1	381:384	arg1	flippase					386:393	the putative Apt1 flippase	368:393	the putative Apt1 flippase	368:393	On the basis of the observations that flippases are required for polysaccharide secretion in plants and the putative Apt1 flippase is required for cryptococcal virulence, we analyzed the role of this enzyme in polysaccharide release by C. neoformans, using a previously characterized apt1Δ mutant.
24337112	0	5	from	Role	0:3	arg1	secretion					43:51	polysaccharide secretion	28:51	polysaccharide secretion by Cryptococcus neoformans	28:78	Role of the Apt1 protein in polysaccharide secretion by Cryptococcus neoformans.
24337112	5	6	with	comparison	892:901	arg1	those					908:912	those	908:912	those	908:912	The apt1Δ mutant, however, produced extracellular vesicles (EVs) with a lower GXM content and different size distribution in comparison with those of WT cells.
24337112	8	7	theme	brain	1402:1406	arg1	colonization					1382:1393	colonization	1382:1393	colonization of the brain	1382:1406	This phenotype was associated with decreased survival of the mutant in the lungs of infected mice, reduced induction of interleukin-6 (IL-6) cytokine levels, and inefficacy in colonization of the brain.
24337112	4	8	theme	important	601:609	arg1	morphology					657:666	capsule morphology	649:666	capsule morphology	649:666	Mutant and wild-type (WT) cells shared important phenotypic characteristics, including capsule morphology and dimensions, glucuronoxylomannan (GXM) composition, molecular size, and serological properties.
24337112	4	8	theme	important	601:609	arg1	dimensions					672:681	dimensions	672:681	dimensions	672:681	Mutant and wild-type (WT) cells shared important phenotypic characteristics, including capsule morphology and dimensions, glucuronoxylomannan (GXM) composition, molecular size, and serological properties.
24337112	4	8	theme	important	601:609	arg1	composition					710:720	glucuronoxylomannan (GXM) composition	684:720	glucuronoxylomannan (GXM) composition	684:720	Mutant and wild-type (WT) cells shared important phenotypic characteristics, including capsule morphology and dimensions, glucuronoxylomannan (GXM) composition, molecular size, and serological properties.
24337112	4	8	theme	important	601:609	arg1	characteristics					622:636	important phenotypic characteristics	601:636	important phenotypic characteristics	601:636	Mutant and wild-type (WT) cells shared important phenotypic characteristics, including capsule morphology and dimensions, glucuronoxylomannan (GXM) composition, molecular size, and serological properties.
24337112	4	8	theme	important	601:609	arg1	properties					755:764	serological properties	743:764	serological properties	743:764	Mutant and wild-type (WT) cells shared important phenotypic characteristics, including capsule morphology and dimensions, glucuronoxylomannan (GXM) composition, molecular size, and serological properties.
24337112	4	8	theme	important	601:609	arg1	size					733:736	molecular size	723:736	molecular size	723:736	Mutant and wild-type (WT) cells shared important phenotypic characteristics, including capsule morphology and dimensions, glucuronoxylomannan (GXM) composition, molecular size, and serological properties.
24337112	7	9	theme	lung	1187:1190	arg1	colonization					1192:1203	lung colonization	1187:1203	lung colonization	1187:1203	These findings were correlated with diminished GXM production during in vitro growth, macrophage infection, and lung colonization.
24337112	2	10	theme	Vesicular	158:166	arg1	secretion					183:191	Vesicular polysaccharide secretion	158:191	Vesicular polysaccharide secretion	158:191	Vesicular polysaccharide secretion is essential for the pathogenic mechanisms of Cryptococcus neoformans.
24337112	7	11	theme	diminished	1111:1120	arg1	production					1126:1135	diminished GXM production	1111:1135	diminished GXM production during in vitro growth, macrophage infection, and lung colonization	1111:1203	These findings were correlated with diminished GXM production during in vitro growth, macrophage infection, and lung colonization.
24337112	4	12	theme	serological	743:753	arg1	properties					755:764	serological properties	743:764	serological properties	743:764	Mutant and wild-type (WT) cells shared important phenotypic characteristics, including capsule morphology and dimensions, glucuronoxylomannan (GXM) composition, molecular size, and serological properties.
24337112	5	13	theme	different	861:869	arg1	distribution					876:887	different size distribution	861:887	different size distribution	861:887	The apt1Δ mutant, however, produced extracellular vesicles (EVs) with a lower GXM content and different size distribution in comparison with those of WT cells.
24337112	5	14	theme	GXM	845:847	arg1	content					849:855	a lower GXM content	837:855	a lower GXM content	837:855	The apt1Δ mutant, however, produced extracellular vesicles (EVs) with a lower GXM content and different size distribution in comparison with those of WT cells.
24337112	3	15	theme	apt1Δ	548:552	arg1	mutant					554:559	a previously characterized apt1Δ mutant	521:559	a previously characterized apt1Δ mutant	521:559	On the basis of the observations that flippases are required for polysaccharide secretion in plants and the putative Apt1 flippase is required for cryptococcal virulence, we analyzed the role of this enzyme in polysaccharide release by C. neoformans, using a previously characterized apt1Δ mutant.
24337112	4	16	theme	glucuronoxylomannan	684:702	arg1	composition					710:720	glucuronoxylomannan (GXM) composition	684:720	glucuronoxylomannan (GXM) composition	684:720	Mutant and wild-type (WT) cells shared important phenotypic characteristics, including capsule morphology and dimensions, glucuronoxylomannan (GXM) composition, molecular size, and serological properties.
24337112	3	17	theme	putative	372:379	arg1	flippase					386:393	the putative Apt1 flippase	368:393	the putative Apt1 flippase	368:393	On the basis of the observations that flippases are required for polysaccharide secretion in plants and the putative Apt1 flippase is required for cryptococcal virulence, we analyzed the role of this enzyme in polysaccharide release by C. neoformans, using a previously characterized apt1Δ mutant.
24337112	4	18	dep	wild-type	573:581	arg1	WT					584:585	WT	584:585	WT	584:585	Mutant and wild-type (WT) cells shared important phenotypic characteristics, including capsule morphology and dimensions, glucuronoxylomannan (GXM) composition, molecular size, and serological properties.
24337112	7	19	dep	in	1144:1145	arg1	vitro					1147:1151	vitro	1147:1151	vitro	1147:1151	These findings were correlated with diminished GXM production during in vitro growth, macrophage infection, and lung colonization.
24337112	9	20	theme	APT1	1463:1466	arg1	lack					1455:1458	the lack	1451:1458	the lack of APT1	1451:1466	Taken together, our results indicate that the lack of APT1 caused defects in both GXM synthesis and vesicular export to the extracellular milieu by C. neoformans via processes that are apparently related to the pathogenic mechanisms used by this fungus during animal infection.
24337112	5	21	theme	size	871:874	arg1	distribution					876:887	different size distribution	861:887	different size distribution	861:887	The apt1Δ mutant, however, produced extracellular vesicles (EVs) with a lower GXM content and different size distribution in comparison with those of WT cells.
24337112	8	22	theme	mice	1299:1302	arg1	lungs					1281:1285	the lungs	1277:1285	the lungs of infected mice	1277:1302	This phenotype was associated with decreased survival of the mutant in the lungs of infected mice, reduced induction of interleukin-6 (IL-6) cytokine levels, and inefficacy in colonization of the brain.
24337112	9	23	theme	GXM	1491:1493	arg1	synthesis					1495:1503	GXM synthesis	1491:1503	GXM synthesis	1491:1503	Taken together, our results indicate that the lack of APT1 caused defects in both GXM synthesis and vesicular export to the extracellular milieu by C. neoformans via processes that are apparently related to the pathogenic mechanisms used by this fungus during animal infection.
24337112	8	24	from	induction	1313:1321	arg1	colonization					1382:1393	colonization	1382:1393	colonization of the brain	1382:1406	This phenotype was associated with decreased survival of the mutant in the lungs of infected mice, reduced induction of interleukin-6 (IL-6) cytokine levels, and inefficacy in colonization of the brain.
24337112	8	24	from	induction	1313:1321	arg1	lungs					1281:1285	the lungs	1277:1285	the lungs of infected mice	1277:1302	This phenotype was associated with decreased survival of the mutant in the lungs of infected mice, reduced induction of interleukin-6 (IL-6) cytokine levels, and inefficacy in colonization of the brain.
24337112	8	25	theme	levels	1356:1361	arg1	survival					1251:1258	decreased survival	1241:1258	decreased survival of the mutant in the lungs of infected mice	1241:1302	This phenotype was associated with decreased survival of the mutant in the lungs of infected mice, reduced induction of interleukin-6 (IL-6) cytokine levels, and inefficacy in colonization of the brain.
24337112	8	25	theme	levels	1356:1361	arg1	inefficacy					1368:1377	inefficacy	1368:1377	inefficacy in colonization of the brain	1368:1406	This phenotype was associated with decreased survival of the mutant in the lungs of infected mice, reduced induction of interleukin-6 (IL-6) cytokine levels, and inefficacy in colonization of the brain.
24337112	8	25	theme	levels	1356:1361	arg1	induction					1313:1321	reduced induction	1305:1321	reduced induction of interleukin-6 (IL-6) cytokine levels	1305:1361	This phenotype was associated with decreased survival of the mutant in the lungs of infected mice, reduced induction of interleukin-6 (IL-6) cytokine levels, and inefficacy in colonization of the brain.
24337112	8	26	from	inefficacy	1368:1377	arg1	colonization					1382:1393	colonization	1382:1393	colonization of the brain	1382:1406	This phenotype was associated with decreased survival of the mutant in the lungs of infected mice, reduced induction of interleukin-6 (IL-6) cytokine levels, and inefficacy in colonization of the brain.
24337112	8	26	from	inefficacy	1368:1377	arg1	lungs					1281:1285	the lungs	1277:1285	the lungs of infected mice	1277:1302	This phenotype was associated with decreased survival of the mutant in the lungs of infected mice, reduced induction of interleukin-6 (IL-6) cytokine levels, and inefficacy in colonization of the brain.
24337112	6	27	theme	Golgi	1058:1062	arg1	apparatus					1064:1072	the Golgi apparatus	1054:1072	the Golgi apparatus	1054:1072	Our data also suggested a defective intracellular GXM synthesis in mutant cells, in addition to changes in the architecture of the Golgi apparatus.
24337112	3	28	theme	cryptococcal	411:422	arg1	virulence					424:432	cryptococcal virulence	411:432	cryptococcal virulence	411:432	On the basis of the observations that flippases are required for polysaccharide secretion in plants and the putative Apt1 flippase is required for cryptococcal virulence, we analyzed the role of this enzyme in polysaccharide release by C. neoformans, using a previously characterized apt1Δ mutant.
24337112	4	29	theme	wild-type	573:581	arg1	cells					588:592	Mutant and wild-type (WT) cells	562:592	Mutant and wild-type (WT) cells	562:592	Mutant and wild-type (WT) cells shared important phenotypic characteristics, including capsule morphology and dimensions, glucuronoxylomannan (GXM) composition, molecular size, and serological properties.
24337112	8	30	theme	decreased	1241:1249	arg1	survival					1251:1258	decreased survival	1241:1258	decreased survival of the mutant in the lungs of infected mice	1241:1302	This phenotype was associated with decreased survival of the mutant in the lungs of infected mice, reduced induction of interleukin-6 (IL-6) cytokine levels, and inefficacy in colonization of the brain.
24337112	8	31	theme	cytokine	1347:1354	arg1	levels					1356:1361	interleukin-6 (IL-6) cytokine levels	1326:1361	interleukin-6 (IL-6) cytokine levels	1326:1361	This phenotype was associated with decreased survival of the mutant in the lungs of infected mice, reduced induction of interleukin-6 (IL-6) cytokine levels, and inefficacy in colonization of the brain.
24337112	6	32	from	changes	1023:1029	arg1	architecture					1038:1049	the architecture	1034:1049	the architecture of the Golgi apparatus	1034:1072	Our data also suggested a defective intracellular GXM synthesis in mutant cells, in addition to changes in the architecture of the Golgi apparatus.
24337112	0	33	theme	Apt1	12:15	arg1	protein					17:23	the Apt1 protein	8:23	the Apt1 protein	8:23	Role of the Apt1 protein in polysaccharide secretion by Cryptococcus neoformans.
24337112	6	34	theme	GXM	977:979	arg1	synthesis					981:989	a defective intracellular GXM synthesis	951:989	a defective intracellular GXM synthesis in mutant cells	951:1005	Our data also suggested a defective intracellular GXM synthesis in mutant cells, in addition to changes in the architecture of the Golgi apparatus.
24337112	8	35	theme	infected	1290:1297	arg1	mice					1299:1302	infected mice	1290:1302	infected mice	1290:1302	This phenotype was associated with decreased survival of the mutant in the lungs of infected mice, reduced induction of interleukin-6 (IL-6) cytokine levels, and inefficacy in colonization of the brain.
24337112	8	36	from	lungs	1281:1285	arg1	survival					1251:1258	decreased survival	1241:1258	decreased survival of the mutant in the lungs of infected mice	1241:1302	This phenotype was associated with decreased survival of the mutant in the lungs of infected mice, reduced induction of interleukin-6 (IL-6) cytokine levels, and inefficacy in colonization of the brain.
24337112	8	36	from	lungs	1281:1285	arg1	inefficacy					1368:1377	inefficacy	1368:1377	inefficacy in colonization of the brain	1368:1406	This phenotype was associated with decreased survival of the mutant in the lungs of infected mice, reduced induction of interleukin-6 (IL-6) cytokine levels, and inefficacy in colonization of the brain.
24337112	8	36	from	lungs	1281:1285	arg1	induction					1313:1321	reduced induction	1305:1321	reduced induction of interleukin-6 (IL-6) cytokine levels	1305:1361	This phenotype was associated with decreased survival of the mutant in the lungs of infected mice, reduced induction of interleukin-6 (IL-6) cytokine levels, and inefficacy in colonization of the brain.
24337112	1	37	theme	key	95:97	arg1	Flippases					81:89	Flippases	81:89	Flippases	81:89	Flippases are key regulators of membrane asymmetry and secretory mechanisms.
24337112	1	37	theme	key	95:97	arg1	regulators					99:108	key regulators	95:108	key regulators of membrane asymmetry and secretory mechanisms	95:155	Flippases are key regulators of membrane asymmetry and secretory mechanisms.
24337112	3	38	theme	polysaccharide	474:487	arg1	release					489:495	polysaccharide release	474:495	polysaccharide release	474:495	On the basis of the observations that flippases are required for polysaccharide secretion in plants and the putative Apt1 flippase is required for cryptococcal virulence, we analyzed the role of this enzyme in polysaccharide release by C. neoformans, using a previously characterized apt1Δ mutant.
24337112	7	39	theme	macrophage	1161:1170	arg1	infection					1172:1180	macrophage infection	1161:1180	macrophage infection	1161:1180	These findings were correlated with diminished GXM production during in vitro growth, macrophage infection, and lung colonization.
24337112	6	40	theme	intracellular	963:975	arg1	synthesis					981:989	a defective intracellular GXM synthesis	951:989	a defective intracellular GXM synthesis in mutant cells	951:1005	Our data also suggested a defective intracellular GXM synthesis in mutant cells, in addition to changes in the architecture of the Golgi apparatus.
24337112	8	41	theme	mutant	1267:1272	arg1	survival					1251:1258	decreased survival	1241:1258	decreased survival of the mutant in the lungs of infected mice	1241:1302	This phenotype was associated with decreased survival of the mutant in the lungs of infected mice, reduced induction of interleukin-6 (IL-6) cytokine levels, and inefficacy in colonization of the brain.
24337112	8	41	theme	mutant	1267:1272	arg1	inefficacy					1368:1377	inefficacy	1368:1377	inefficacy in colonization of the brain	1368:1406	This phenotype was associated with decreased survival of the mutant in the lungs of infected mice, reduced induction of interleukin-6 (IL-6) cytokine levels, and inefficacy in colonization of the brain.
24337112	8	41	theme	mutant	1267:1272	arg1	induction					1313:1321	reduced induction	1305:1321	reduced induction of interleukin-6 (IL-6) cytokine levels	1305:1361	This phenotype was associated with decreased survival of the mutant in the lungs of infected mice, reduced induction of interleukin-6 (IL-6) cytokine levels, and inefficacy in colonization of the brain.
24337112	9	42	theme	extracellular	1533:1545	arg1	milieu					1547:1552	the extracellular milieu	1529:1552	the extracellular milieu by C. neoformans via processes that are apparently related to the pathogenic mechanisms used by this fungus during animal infection	1529:1684	Taken together, our results indicate that the lack of APT1 caused defects in both GXM synthesis and vesicular export to the extracellular milieu by C. neoformans via processes that are apparently related to the pathogenic mechanisms used by this fungus during animal infection.
24337112	4	43	theme	capsule	649:655	arg1	morphology					657:666	capsule morphology	649:666	capsule morphology	649:666	Mutant and wild-type (WT) cells shared important phenotypic characteristics, including capsule morphology and dimensions, glucuronoxylomannan (GXM) composition, molecular size, and serological properties.
24337112	9	44	theme	pathogenic	1620:1629	arg1	mechanisms					1631:1640	the pathogenic mechanisms	1616:1640	the pathogenic mechanisms used by this fungus during animal infection	1616:1684	Taken together, our results indicate that the lack of APT1 caused defects in both GXM synthesis and vesicular export to the extracellular milieu by C. neoformans via processes that are apparently related to the pathogenic mechanisms used by this fungus during animal infection.
24337112	6	45	theme	defective	953:961	arg1	synthesis					981:989	a defective intracellular GXM synthesis	951:989	a defective intracellular GXM synthesis in mutant cells	951:1005	Our data also suggested a defective intracellular GXM synthesis in mutant cells, in addition to changes in the architecture of the Golgi apparatus.
24337112	6	46	from	synthesis	981:989	arg1	cells					1001:1005	mutant cells	994:1005	mutant cells	994:1005	Our data also suggested a defective intracellular GXM synthesis in mutant cells, in addition to changes in the architecture of the Golgi apparatus.
24337112	3	47	theme	enzyme	464:469	arg1	role					451:454	the role	447:454	the role of this enzyme in polysaccharide release	447:495	On the basis of the observations that flippases are required for polysaccharide secretion in plants and the putative Apt1 flippase is required for cryptococcal virulence, we analyzed the role of this enzyme in polysaccharide release by C. neoformans, using a previously characterized apt1Δ mutant.
24337112	0	48	theme	protein	17:23	arg1	Role					0:3	Role	0:3	Role of the Apt1 protein in polysaccharide secretion by Cryptococcus neoformans.	0:79	Role of the Apt1 protein in polysaccharide secretion by Cryptococcus neoformans.
24337112	5	49	theme	extracellular	803:815	arg1	vesicles					817:824	extracellular vesicles	803:824	extracellular vesicles (EVs)	803:830	The apt1Δ mutant, however, produced extracellular vesicles (EVs) with a lower GXM content and different size distribution in comparison with those of WT cells.
24337112	5	49	theme	extracellular	803:815	arg1	EVs					827:829	EVs	827:829	EVs	827:829	The apt1Δ mutant, however, produced extracellular vesicles (EVs) with a lower GXM content and different size distribution in comparison with those of WT cells.
24337112	2	50	theme	neoformans	252:261	arg1	mechanisms					225:234	the pathogenic mechanisms	210:234	the pathogenic mechanisms of Cryptococcus neoformans	210:261	Vesicular polysaccharide secretion is essential for the pathogenic mechanisms of Cryptococcus neoformans.
24337112	5	51	from	content	849:855	arg1	comparison					892:901	comparison	892:901	comparison with those of WT cells	892:924	The apt1Δ mutant, however, produced extracellular vesicles (EVs) with a lower GXM content and different size distribution in comparison with those of WT cells.
24337112	5	52	theme	apt1Δ	771:775	arg1	mutant					777:782	The apt1Δ mutant	767:782	The apt1Δ mutant	767:782	The apt1Δ mutant, however, produced extracellular vesicles (EVs) with a lower GXM content and different size distribution in comparison with those of WT cells.
24337112	2	53	theme	polysaccharide	168:181	arg1	secretion					183:191	Vesicular polysaccharide secretion	158:191	Vesicular polysaccharide secretion	158:191	Vesicular polysaccharide secretion is essential for the pathogenic mechanisms of Cryptococcus neoformans.
24337112	2	54	theme	Cryptococcus	239:250	arg1	neoformans					252:261	Cryptococcus neoformans	239:261	Cryptococcus neoformans	239:261	Vesicular polysaccharide secretion is essential for the pathogenic mechanisms of Cryptococcus neoformans.
24337112	8	55	from	survival	1251:1258	arg1	colonization					1382:1393	colonization	1382:1393	colonization of the brain	1382:1406	This phenotype was associated with decreased survival of the mutant in the lungs of infected mice, reduced induction of interleukin-6 (IL-6) cytokine levels, and inefficacy in colonization of the brain.
24337112	8	55	from	survival	1251:1258	arg1	lungs					1281:1285	the lungs	1277:1285	the lungs of infected mice	1277:1302	This phenotype was associated with decreased survival of the mutant in the lungs of infected mice, reduced induction of interleukin-6 (IL-6) cytokine levels, and inefficacy in colonization of the brain.
24337112	5	56	theme	WT	917:918	arg1	cells					920:924	WT cells	917:924	WT cells	917:924	The apt1Δ mutant, however, produced extracellular vesicles (EVs) with a lower GXM content and different size distribution in comparison with those of WT cells.
24337112	0	57	theme	polysaccharide	28:41	arg1	secretion					43:51	polysaccharide secretion	28:51	polysaccharide secretion by Cryptococcus neoformans	28:78	Role of the Apt1 protein in polysaccharide secretion by Cryptococcus neoformans.
24337112	4	58	theme	Mutant	562:567	arg1	cells					588:592	Mutant and wild-type (WT) cells	562:592	Mutant and wild-type (WT) cells	562:592	Mutant and wild-type (WT) cells shared important phenotypic characteristics, including capsule morphology and dimensions, glucuronoxylomannan (GXM) composition, molecular size, and serological properties.
24337112	3	59	theme	polysaccharide	329:342	arg1	secretion					344:352	polysaccharide secretion	329:352	polysaccharide secretion in plants	329:362	On the basis of the observations that flippases are required for polysaccharide secretion in plants and the putative Apt1 flippase is required for cryptococcal virulence, we analyzed the role of this enzyme in polysaccharide release by C. neoformans, using a previously characterized apt1Δ mutant.
24337112	3	60	from	secretion	344:352	arg1	plants					357:362	plants	357:362	plants	357:362	On the basis of the observations that flippases are required for polysaccharide secretion in plants and the putative Apt1 flippase is required for cryptococcal virulence, we analyzed the role of this enzyme in polysaccharide release by C. neoformans, using a previously characterized apt1Δ mutant.
24337112	1	61	theme	membrane	113:120	arg1	asymmetry					122:130	membrane asymmetry	113:130	membrane asymmetry	113:130	Flippases are key regulators of membrane asymmetry and secretory mechanisms.
24337112	2	62	theme	pathogenic	214:223	arg1	mechanisms					225:234	the pathogenic mechanisms	210:234	the pathogenic mechanisms of Cryptococcus neoformans	210:261	Vesicular polysaccharide secretion is essential for the pathogenic mechanisms of Cryptococcus neoformans.
24337112	7	63	theme	in	1144:1145	arg1	growth					1153:1158	in vitro growth	1144:1158	in vitro growth	1144:1158	These findings were correlated with diminished GXM production during in vitro growth, macrophage infection, and lung colonization.
24337112	1	64	theme	asymmetry	122:130	arg1	Flippases					81:89	Flippases	81:89	Flippases	81:89	Flippases are key regulators of membrane asymmetry and secretory mechanisms.
24337112	1	64	theme	asymmetry	122:130	arg1	regulators					99:108	key regulators	95:108	key regulators of membrane asymmetry and secretory mechanisms	95:155	Flippases are key regulators of membrane asymmetry and secretory mechanisms.
24337112	6	65	theme	mutant	994:999	arg1	cells					1001:1005	mutant cells	994:1005	mutant cells	994:1005	Our data also suggested a defective intracellular GXM synthesis in mutant cells, in addition to changes in the architecture of the Golgi apparatus.
24337112	8	66	theme	reduced	1305:1311	arg1	induction					1313:1321	reduced induction	1305:1321	reduced induction of interleukin-6 (IL-6) cytokine levels	1305:1361	This phenotype was associated with decreased survival of the mutant in the lungs of infected mice, reduced induction of interleukin-6 (IL-6) cytokine levels, and inefficacy in colonization of the brain.
24337112	5	67	from	distribution	876:887	arg1	comparison					892:901	comparison	892:901	comparison with those of WT cells	892:924	The apt1Δ mutant, however, produced extracellular vesicles (EVs) with a lower GXM content and different size distribution in comparison with those of WT cells.
24337112	1	68	theme	mechanisms	146:155	arg1	Flippases					81:89	Flippases	81:89	Flippases	81:89	Flippases are key regulators of membrane asymmetry and secretory mechanisms.
24337112	1	68	theme	mechanisms	146:155	arg1	regulators					99:108	key regulators	95:108	key regulators of membrane asymmetry and secretory mechanisms	95:155	Flippases are key regulators of membrane asymmetry and secretory mechanisms.
24337112	8	69	theme	interleukin-6	1326:1338	arg1	levels					1356:1361	interleukin-6 (IL-6) cytokine levels	1326:1361	interleukin-6 (IL-6) cytokine levels	1326:1361	This phenotype was associated with decreased survival of the mutant in the lungs of infected mice, reduced induction of interleukin-6 (IL-6) cytokine levels, and inefficacy in colonization of the brain.
24337112	4	70	theme	phenotypic	611:620	arg1	morphology					657:666	capsule morphology	649:666	capsule morphology	649:666	Mutant and wild-type (WT) cells shared important phenotypic characteristics, including capsule morphology and dimensions, glucuronoxylomannan (GXM) composition, molecular size, and serological properties.
24337112	4	70	theme	phenotypic	611:620	arg1	dimensions					672:681	dimensions	672:681	dimensions	672:681	Mutant and wild-type (WT) cells shared important phenotypic characteristics, including capsule morphology and dimensions, glucuronoxylomannan (GXM) composition, molecular size, and serological properties.
24337112	4	70	theme	phenotypic	611:620	arg1	composition					710:720	glucuronoxylomannan (GXM) composition	684:720	glucuronoxylomannan (GXM) composition	684:720	Mutant and wild-type (WT) cells shared important phenotypic characteristics, including capsule morphology and dimensions, glucuronoxylomannan (GXM) composition, molecular size, and serological properties.
24337112	4	70	theme	phenotypic	611:620	arg1	characteristics					622:636	important phenotypic characteristics	601:636	important phenotypic characteristics	601:636	Mutant and wild-type (WT) cells shared important phenotypic characteristics, including capsule morphology and dimensions, glucuronoxylomannan (GXM) composition, molecular size, and serological properties.
24337112	4	70	theme	phenotypic	611:620	arg1	properties					755:764	serological properties	743:764	serological properties	743:764	Mutant and wild-type (WT) cells shared important phenotypic characteristics, including capsule morphology and dimensions, glucuronoxylomannan (GXM) composition, molecular size, and serological properties.
24337112	4	70	theme	phenotypic	611:620	arg1	size					733:736	molecular size	723:736	molecular size	723:736	Mutant and wild-type (WT) cells shared important phenotypic characteristics, including capsule morphology and dimensions, glucuronoxylomannan (GXM) composition, molecular size, and serological properties.
24337112	0	71	theme	Cryptococcus	56:67	arg1	neoformans					69:78	Cryptococcus neoformans	56:78	Cryptococcus neoformans	56:78	Role of the Apt1 protein in polysaccharide secretion by Cryptococcus neoformans.
24337112	3	72	from	role	451:454	arg1	release					489:495	polysaccharide release	474:495	polysaccharide release	474:495	On the basis of the observations that flippases are required for polysaccharide secretion in plants and the putative Apt1 flippase is required for cryptococcal virulence, we analyzed the role of this enzyme in polysaccharide release by C. neoformans, using a previously characterized apt1Δ mutant.
24337112	1	73	theme	secretory	136:144	arg1	mechanisms					146:155	secretory mechanisms	136:155	secretory mechanisms	136:155	Flippases are key regulators of membrane asymmetry and secretory mechanisms.
24337112	6	74	theme	apparatus	1064:1072	arg1	architecture					1038:1049	the architecture	1034:1049	the architecture of the Golgi apparatus	1034:1072	Our data also suggested a defective intracellular GXM synthesis in mutant cells, in addition to changes in the architecture of the Golgi apparatus.
24337112	9	75	theme	vesicular	1509:1517	arg1	export					1519:1524	vesicular export	1509:1524	vesicular export	1509:1524	Taken together, our results indicate that the lack of APT1 caused defects in both GXM synthesis and vesicular export to the extracellular milieu by C. neoformans via processes that are apparently related to the pathogenic mechanisms used by this fungus during animal infection.
24337112	3	76	dep	observations	284:295	arg1	the					267:269	the	267:269	the	267:269	On the basis of the observations that flippases are required for polysaccharide secretion in plants and the putative Apt1 flippase is required for cryptococcal virulence, we analyzed the role of this enzyme in polysaccharide release by C. neoformans, using a previously characterized apt1Δ mutant.
24337112	3	76	dep	observations	284:295	arg1	basis					271:275	basis	271:275	basis	271:275	On the basis of the observations that flippases are required for polysaccharide secretion in plants and the putative Apt1 flippase is required for cryptococcal virulence, we analyzed the role of this enzyme in polysaccharide release by C. neoformans, using a previously characterized apt1Δ mutant.
24337112	3	76	dep	observations	284:295	arg1	required					398:405	required	398:405	is required for cryptococcal virulence	395:432	On the basis of the observations that flippases are required for polysaccharide secretion in plants and the putative Apt1 flippase is required for cryptococcal virulence, we analyzed the role of this enzyme in polysaccharide release by C. neoformans, using a previously characterized apt1Δ mutant.
24337112	3	76	dep	observations	284:295	arg1	required					316:323	required	316:323	are required for polysaccharide secretion in plants	312:362	On the basis of the observations that flippases are required for polysaccharide secretion in plants and the putative Apt1 flippase is required for cryptococcal virulence, we analyzed the role of this enzyme in polysaccharide release by C. neoformans, using a previously characterized apt1Δ mutant.
24337112	8	77	from	mutant	1267:1272	arg1	lungs					1281:1285	the lungs	1277:1285	the lungs of infected mice	1277:1302	This phenotype was associated with decreased survival of the mutant in the lungs of infected mice, reduced induction of interleukin-6 (IL-6) cytokine levels, and inefficacy in colonization of the brain.
24337112	3	78	theme	characterized	534:546	arg1	mutant					554:559	a previously characterized apt1Δ mutant	521:559	a previously characterized apt1Δ mutant	521:559	On the basis of the observations that flippases are required for polysaccharide secretion in plants and the putative Apt1 flippase is required for cryptococcal virulence, we analyzed the role of this enzyme in polysaccharide release by C. neoformans, using a previously characterized apt1Δ mutant.
24337112	9	79	from	defects	1475:1481	arg1	export					1519:1524	vesicular export	1509:1524	vesicular export	1509:1524	Taken together, our results indicate that the lack of APT1 caused defects in both GXM synthesis and vesicular export to the extracellular milieu by C. neoformans via processes that are apparently related to the pathogenic mechanisms used by this fungus during animal infection.
24337112	9	79	from	defects	1475:1481	arg1	synthesis					1495:1503	GXM synthesis	1491:1503	GXM synthesis	1491:1503	Taken together, our results indicate that the lack of APT1 caused defects in both GXM synthesis and vesicular export to the extracellular milieu by C. neoformans via processes that are apparently related to the pathogenic mechanisms used by this fungus during animal infection.
26593492	1	0	theme	relative	312:319	arg1	values					321:326	relative values	312:326	relative values	312:326	Increasing glucose concentration (up to 46%) in the culture medium of Volvariella volvacea resulted in a noticeable biomass increase (63.82%) and intracellular polysaccharide (IPS) production (32.84% in absolute and 51.34% in relative values).
26593492	6	1	theme	fatty	777:781	arg1	acids					783:787	saturated fatty acids	767:787	saturated fatty acids	767:787	Phospholipid was the richest in saturated fatty acids lipid fraction.
26593492	0	2	theme	ratio	73:77	arg1	media					79:83	nitrogen ratio media	64:83	nitrogen ratio media	64:83	Adaptation of Volvariella volvacea metabolism in high carbon to nitrogen ratio media.
26593492	5	3	theme	Fatty	565:569	arg1	composition					576:586	Fatty acid composition	565:586	Fatty acid composition of lipids	565:596	Fatty acid composition of lipids was not remarkably affected by the C/N ratio imposed, whereas the concentration of neutral lipids increased in the high C/N ratio media.
26593492	9	4	theme	IPS	1102:1104	arg1	composition					1106:1116	IPS composition	1102:1116	IPS composition	1102:1116	C/N ratio and fermentation time affected IPS composition, as glucose increased in a high C/N ratio, while fructose and mannitol decreased.
26593492	2	5	from	yields	380:385	arg1	glucose					390:396	glucose	390:396	glucose consumed	390:405	In high glucose concentrations, decreased biomass yields on glucose consumed were recorded.
26593492	9	6	theme	high	1145:1148	arg1	ratio					1154:1158	a high C/N ratio	1143:1158	a high C/N ratio	1143:1158	C/N ratio and fermentation time affected IPS composition, as glucose increased in a high C/N ratio, while fructose and mannitol decreased.
26593492	5	7	theme	C/N	633:635	arg1	ratio					637:641	the C/N ratio	629:641	the C/N ratio imposed	629:649	Fatty acid composition of lipids was not remarkably affected by the C/N ratio imposed, whereas the concentration of neutral lipids increased in the high C/N ratio media.
26593492	0	8	from	Adaptation	0:9	arg1	carbon					54:59	high carbon	49:59	high carbon to nitrogen ratio media	49:83	Adaptation of Volvariella volvacea metabolism in high carbon to nitrogen ratio media.
26593492	5	9	theme	acid	571:574	arg1	composition					576:586	Fatty acid composition	565:586	Fatty acid composition of lipids	565:596	Fatty acid composition of lipids was not remarkably affected by the C/N ratio imposed, whereas the concentration of neutral lipids increased in the high C/N ratio media.
26593492	7	10	dep	phosphatidyl-choline	842:861	arg1	i.e.					837:840	i.e.	837:840	i.e.	837:840	The major phospholipid classes, i.e. phosphatidyl-choline and phosphatidyl-inositol, were differentially affected by the high glucose concentrations, as the former increased its proportion and the latter decreased.
26593492	1	11	dep	46	126:127	arg1	to					123:124	to	123:124	to	123:124	Increasing glucose concentration (up to 46%) in the culture medium of Volvariella volvacea resulted in a noticeable biomass increase (63.82%) and intracellular polysaccharide (IPS) production (32.84% in absolute and 51.34% in relative values).
26593492	6	12	theme	lipid	789:793	arg1	Phospholipid					735:746	Phospholipid	735:746	Phospholipid	735:746	Phospholipid was the richest in saturated fatty acids lipid fraction.
26593492	6	12	theme	lipid	789:793	arg1	fraction					795:802	the richest in saturated fatty acids lipid fraction	752:802	the richest in saturated fatty acids lipid fraction	752:802	Phospholipid was the richest in saturated fatty acids lipid fraction.
26593492	1	13	theme	culture	138:144	arg1	medium					146:151	the culture medium	134:151	the culture medium of Volvariella volvacea	134:175	Increasing glucose concentration (up to 46%) in the culture medium of Volvariella volvacea resulted in a noticeable biomass increase (63.82%) and intracellular polysaccharide (IPS) production (32.84% in absolute and 51.34% in relative values).
26593492	6	14	from	richest	756:762	arg1	acids					783:787	saturated fatty acids	767:787	saturated fatty acids	767:787	Phospholipid was the richest in saturated fatty acids lipid fraction.
26593492	1	15	dep	production	267:276	arg1	%					307:307	51.34%	302:307	51.34% in relative values	302:326	Increasing glucose concentration (up to 46%) in the culture medium of Volvariella volvacea resulted in a noticeable biomass increase (63.82%) and intracellular polysaccharide (IPS) production (32.84% in absolute and 51.34% in relative values).
26593492	1	15	dep	production	267:276	arg1	%					284:284	32.84%	279:284	32.84% in absolute	279:296	Increasing glucose concentration (up to 46%) in the culture medium of Volvariella volvacea resulted in a noticeable biomass increase (63.82%) and intracellular polysaccharide (IPS) production (32.84% in absolute and 51.34% in relative values).
26593492	5	16	theme	high	713:716	arg1	ratio					722:726	high C/N ratio	713:726	the high C/N ratio media	709:732	Fatty acid composition of lipids was not remarkably affected by the C/N ratio imposed, whereas the concentration of neutral lipids increased in the high C/N ratio media.
26593492	1	17	theme	intracellular	232:244	arg1	production					267:276	intracellular polysaccharide (IPS) production	232:276	intracellular polysaccharide (IPS) production (32.84% in absolute and 51.34% in relative values)	232:327	Increasing glucose concentration (up to 46%) in the culture medium of Volvariella volvacea resulted in a noticeable biomass increase (63.82%) and intracellular polysaccharide (IPS) production (32.84% in absolute and 51.34% in relative values).
26593492	3	18	dep	synthesized	449:459	arg1	%					473:473	up to 32.22%	462:473	up to 32.22%	462:473	A non-negligible lipid was synthesized (up to 32.22%, w/w) in the first growth steps.
26593492	3	18	dep	synthesized	449:459	arg1	w/w					476:478	w/w	476:478	w/w	476:478	A non-negligible lipid was synthesized (up to 32.22%, w/w) in the first growth steps.
26593492	1	19	theme	Volvariella	156:166	arg1	volvacea					168:175	Volvariella volvacea	156:175	Volvariella volvacea	156:175	Increasing glucose concentration (up to 46%) in the culture medium of Volvariella volvacea resulted in a noticeable biomass increase (63.82%) and intracellular polysaccharide (IPS) production (32.84% in absolute and 51.34% in relative values).
26593492	2	20	theme	biomass	372:378	arg1	yields					380:385	decreased biomass yields	362:385	decreased biomass yields on glucose consumed	362:405	In high glucose concentrations, decreased biomass yields on glucose consumed were recorded.
26593492	1	21	theme	polysaccharide	246:259	arg1	production					267:276	intracellular polysaccharide (IPS) production	232:276	intracellular polysaccharide (IPS) production (32.84% in absolute and 51.34% in relative values)	232:327	Increasing glucose concentration (up to 46%) in the culture medium of Volvariella volvacea resulted in a noticeable biomass increase (63.82%) and intracellular polysaccharide (IPS) production (32.84% in absolute and 51.34% in relative values).
26593492	1	22	theme	glucose	97:103	arg1	%					128:128	up to 46%	120:128	up to 46%	120:128	Increasing glucose concentration (up to 46%) in the culture medium of Volvariella volvacea resulted in a noticeable biomass increase (63.82%) and intracellular polysaccharide (IPS) production (32.84% in absolute and 51.34% in relative values).
26593492	1	22	theme	glucose	97:103	arg1	concentration					105:117	glucose concentration	97:117	glucose concentration (up to 46%)	97:129	Increasing glucose concentration (up to 46%) in the culture medium of Volvariella volvacea resulted in a noticeable biomass increase (63.82%) and intracellular polysaccharide (IPS) production (32.84% in absolute and 51.34% in relative values).
26593492	1	23	theme	volvacea	168:175	arg1	medium					146:151	the culture medium	134:151	the culture medium of Volvariella volvacea	134:175	Increasing glucose concentration (up to 46%) in the culture medium of Volvariella volvacea resulted in a noticeable biomass increase (63.82%) and intracellular polysaccharide (IPS) production (32.84% in absolute and 51.34% in relative values).
26593492	1	24	from	%	307:307	arg1	absolute					289:296	absolute	289:296	absolute	289:296	Increasing glucose concentration (up to 46%) in the culture medium of Volvariella volvacea resulted in a noticeable biomass increase (63.82%) and intracellular polysaccharide (IPS) production (32.84% in absolute and 51.34% in relative values).
26593492	1	24	from	%	307:307	arg1	values					321:326	relative values	312:326	relative values	312:326	Increasing glucose concentration (up to 46%) in the culture medium of Volvariella volvacea resulted in a noticeable biomass increase (63.82%) and intracellular polysaccharide (IPS) production (32.84% in absolute and 51.34% in relative values).
26593492	9	25	theme	C/N	1061:1063	arg1	ratio					1065:1069	C/N ratio	1061:1069	C/N ratio	1061:1069	C/N ratio and fermentation time affected IPS composition, as glucose increased in a high C/N ratio, while fructose and mannitol decreased.
26593492	6	26	theme	saturated	767:775	arg1	acids					783:787	saturated fatty acids	767:787	saturated fatty acids	767:787	Phospholipid was the richest in saturated fatty acids lipid fraction.
26593492	5	27	theme	C/N	718:720	arg1	ratio					722:726	high C/N ratio	713:726	the high C/N ratio media	709:732	Fatty acid composition of lipids was not remarkably affected by the C/N ratio imposed, whereas the concentration of neutral lipids increased in the high C/N ratio media.
26593492	5	28	theme	neutral	681:687	arg1	lipids					689:694	neutral lipids	681:694	neutral lipids	681:694	Fatty acid composition of lipids was not remarkably affected by the C/N ratio imposed, whereas the concentration of neutral lipids increased in the high C/N ratio media.
26593492	9	29	theme	C/N	1150:1152	arg1	ratio					1154:1158	a high C/N ratio	1143:1158	a high C/N ratio	1143:1158	C/N ratio and fermentation time affected IPS composition, as glucose increased in a high C/N ratio, while fructose and mannitol decreased.
26593492	2	30	theme	glucose	338:344	arg1	concentrations					346:359	high glucose concentrations	333:359	high glucose concentrations	333:359	In high glucose concentrations, decreased biomass yields on glucose consumed were recorded.
26593492	0	31	theme	metabolism	35:44	arg1	Adaptation					0:9	Adaptation	0:9	Adaptation of Volvariella volvacea metabolism in high carbon to nitrogen ratio media.	0:84	Adaptation of Volvariella volvacea metabolism in high carbon to nitrogen ratio media.
26593492	5	32	theme	lipids	689:694	arg1	concentration					664:676	the concentration	660:676	the concentration of neutral lipids	660:694	Fatty acid composition of lipids was not remarkably affected by the C/N ratio imposed, whereas the concentration of neutral lipids increased in the high C/N ratio media.
26593492	6	33	theme	richest	756:762	arg1	Phospholipid					735:746	Phospholipid	735:746	Phospholipid	735:746	Phospholipid was the richest in saturated fatty acids lipid fraction.
26593492	6	33	theme	richest	756:762	arg1	fraction					795:802	the richest in saturated fatty acids lipid fraction	752:802	the richest in saturated fatty acids lipid fraction	752:802	Phospholipid was the richest in saturated fatty acids lipid fraction.
26593492	0	34	theme	volvacea	26:33	arg1	metabolism					35:44	Volvariella volvacea metabolism	14:44	Volvariella volvacea metabolism	14:44	Adaptation of Volvariella volvacea metabolism in high carbon to nitrogen ratio media.
26593492	2	35	theme	high	333:336	arg1	concentrations					346:359	high glucose concentrations	333:359	high glucose concentrations	333:359	In high glucose concentrations, decreased biomass yields on glucose consumed were recorded.
26593492	1	36	from	%	284:284	arg1	absolute					289:296	absolute	289:296	absolute	289:296	Increasing glucose concentration (up to 46%) in the culture medium of Volvariella volvacea resulted in a noticeable biomass increase (63.82%) and intracellular polysaccharide (IPS) production (32.84% in absolute and 51.34% in relative values).
26593492	1	36	from	%	284:284	arg1	values					321:326	relative values	312:326	relative values	312:326	Increasing glucose concentration (up to 46%) in the culture medium of Volvariella volvacea resulted in a noticeable biomass increase (63.82%) and intracellular polysaccharide (IPS) production (32.84% in absolute and 51.34% in relative values).
26593492	6	37	from	acids	783:787	arg1	richest					756:762	richest	756:762	richest	756:762	Phospholipid was the richest in saturated fatty acids lipid fraction.
26593492	3	38	theme	first	488:492	arg1	steps					501:505	the first growth steps	484:505	the first growth steps	484:505	A non-negligible lipid was synthesized (up to 32.22%, w/w) in the first growth steps.
26593492	3	39	theme	non-negligible	424:437	arg1	lipid					439:443	A non-negligible lipid	422:443	A non-negligible lipid	422:443	A non-negligible lipid was synthesized (up to 32.22%, w/w) in the first growth steps.
26593492	0	40	theme	high	49:52	arg1	carbon					54:59	high carbon	49:59	high carbon to nitrogen ratio media	49:83	Adaptation of Volvariella volvacea metabolism in high carbon to nitrogen ratio media.
26593492	5	41	theme	lipids	591:596	arg1	composition					576:586	Fatty acid composition	565:586	Fatty acid composition of lipids	565:596	Fatty acid composition of lipids was not remarkably affected by the C/N ratio imposed, whereas the concentration of neutral lipids increased in the high C/N ratio media.
26593492	3	42	theme	growth	494:499	arg1	steps					501:505	the first growth steps	484:505	the first growth steps	484:505	A non-negligible lipid was synthesized (up to 32.22%, w/w) in the first growth steps.
26593492	7	43	theme	high	926:929	arg1	concentrations					939:952	the high glucose concentrations	922:952	the high glucose concentrations	922:952	The major phospholipid classes, i.e. phosphatidyl-choline and phosphatidyl-inositol, were differentially affected by the high glucose concentrations, as the former increased its proportion and the latter decreased.
26593492	3	44	dep	32.22	468:472	arg1	to					465:466	to	465:466	to	465:466	A non-negligible lipid was synthesized (up to 32.22%, w/w) in the first growth steps.
26593492	7	45	theme	major	809:813	arg1	classes					828:834	The major phospholipid classes	805:834	The major phospholipid classes	805:834	The major phospholipid classes, i.e. phosphatidyl-choline and phosphatidyl-inositol, were differentially affected by the high glucose concentrations, as the former increased its proportion and the latter decreased.
26593492	8	46	theme	fatty	1049:1053	arg1	acid					1055:1058	fatty acid	1049:1058	fatty acid	1049:1058	Linoleic was the predominate fatty acid.
26593492	7	47	theme	glucose	931:937	arg1	concentrations					939:952	the high glucose concentrations	922:952	the high glucose concentrations	922:952	The major phospholipid classes, i.e. phosphatidyl-choline and phosphatidyl-inositol, were differentially affected by the high glucose concentrations, as the former increased its proportion and the latter decreased.
26593492	7	48	theme	phospholipid	815:826	arg1	classes					828:834	The major phospholipid classes	805:834	The major phospholipid classes	805:834	The major phospholipid classes, i.e. phosphatidyl-choline and phosphatidyl-inositol, were differentially affected by the high glucose concentrations, as the former increased its proportion and the latter decreased.
26593492	1	49	theme	noticeable	191:200	arg1	increase					210:217	a noticeable biomass increase	189:217	a noticeable biomass increase (63.82%)	189:226	Increasing glucose concentration (up to 46%) in the culture medium of Volvariella volvacea resulted in a noticeable biomass increase (63.82%) and intracellular polysaccharide (IPS) production (32.84% in absolute and 51.34% in relative values).
26593492	1	49	theme	noticeable	191:200	arg1	%					225:225	63.82%	220:225	63.82%	220:225	Increasing glucose concentration (up to 46%) in the culture medium of Volvariella volvacea resulted in a noticeable biomass increase (63.82%) and intracellular polysaccharide (IPS) production (32.84% in absolute and 51.34% in relative values).
26593492	9	50	theme	fermentation	1075:1086	arg1	time					1088:1091	fermentation time	1075:1091	fermentation time	1075:1091	C/N ratio and fermentation time affected IPS composition, as glucose increased in a high C/N ratio, while fructose and mannitol decreased.
26593492	5	51	theme	ratio	722:726	arg1	media					728:732	the high C/N ratio media	709:732	the high C/N ratio media	709:732	Fatty acid composition of lipids was not remarkably affected by the C/N ratio imposed, whereas the concentration of neutral lipids increased in the high C/N ratio media.
26593492	1	52	theme	biomass	202:208	arg1	increase					210:217	a noticeable biomass increase	189:217	a noticeable biomass increase (63.82%)	189:226	Increasing glucose concentration (up to 46%) in the culture medium of Volvariella volvacea resulted in a noticeable biomass increase (63.82%) and intracellular polysaccharide (IPS) production (32.84% in absolute and 51.34% in relative values).
26593492	1	52	theme	biomass	202:208	arg1	%					225:225	63.82%	220:225	63.82%	220:225	Increasing glucose concentration (up to 46%) in the culture medium of Volvariella volvacea resulted in a noticeable biomass increase (63.82%) and intracellular polysaccharide (IPS) production (32.84% in absolute and 51.34% in relative values).
26593492	0	53	theme	nitrogen	64:71	arg1	media					79:83	nitrogen ratio media	64:83	nitrogen ratio media	64:83	Adaptation of Volvariella volvacea metabolism in high carbon to nitrogen ratio media.
26593492	2	54	theme	decreased	362:370	arg1	yields					380:385	decreased biomass yields	362:385	decreased biomass yields on glucose consumed	362:405	In high glucose concentrations, decreased biomass yields on glucose consumed were recorded.
26593492	0	55	theme	Volvariella	14:24	arg1	metabolism					35:44	Volvariella volvacea metabolism	14:44	Volvariella volvacea metabolism	14:44	Adaptation of Volvariella volvacea metabolism in high carbon to nitrogen ratio media.
27339322	9	0	theme	film	1256:1259	arg1	position					1240:1247	temperature position	1228:1247	temperature position of the film reinforced with 8% NF	1228:1281	The most eminent record was +18.5°C shift in temperature position of the film reinforced with 8% NF.
27339322	10	1	from	ability	1364:1370	arg1	matrix					1347:1352	the matrix	1343:1352	the matrix	1343:1352	This finding implied efficient dispersion of nanofibers in the matrix and their ability to form a network and restrict mobility of the system.
27339322	7	2	dep	10wt	1033:1036	arg1	to					1030:1031	to	1030:1031	to	1030:1031	Tensile strength and Young's modulus increased by 313% and 343%, respectively, with increasing NF content from 0 to 10wt%.
27339322	9	3	theme	8	1277:1277	arg1	%					1278:1278	%	1278:1278	%	1278:1278	The most eminent record was +18.5°C shift in temperature position of the film reinforced with 8% NF.
27339322	1	4	theme	varying	238:244	arg1	contents					246:253	varying contents	238:253	varying contents (0-10wt%)	238:263	Cellulosic nanofibers (NFs) from kenaf bast were used to reinforce glycerol plasticized thermoplastic starch (TPS) matrices with varying contents (0-10wt%).
27339322	1	4	theme	varying	238:244	arg1	%					262:262	0-10wt%	256:262	0-10wt%	256:262	Cellulosic nanofibers (NFs) from kenaf bast were used to reinforce glycerol plasticized thermoplastic starch (TPS) matrices with varying contents (0-10wt%).
27339322	4	5	theme	dimension	475:483	arg1	effects					457:463	the effects	453:463	the effects of filler dimension and loading on linear and non-linear mechanical performance of fabricated materials	453:567	The aim of study was to investigate the effects of filler dimension and loading on linear and non-linear mechanical performance of fabricated materials.
27339322	10	6	theme	system	1419:1424	arg1	mobility					1403:1410	mobility	1403:1410	mobility of the system	1403:1424	This finding implied efficient dispersion of nanofibers in the matrix and their ability to form a network and restrict mobility of the system.
27339322	1	7	from	bast	148:151	arg1	NFs					132:134	NFs	132:134	NFs	132:134	Cellulosic nanofibers (NFs) from kenaf bast were used to reinforce glycerol plasticized thermoplastic starch (TPS) matrices with varying contents (0-10wt%).
27339322	1	7	from	bast	148:151	arg1	nanofibers					120:129	Cellulosic nanofibers	109:129	Cellulosic nanofibers (NFs) from kenaf bast	109:151	Cellulosic nanofibers (NFs) from kenaf bast were used to reinforce glycerol plasticized thermoplastic starch (TPS) matrices with varying contents (0-10wt%).
27339322	5	8	theme	mechanical	668:677	arg1	performance					679:689	significantly greater mechanical performance	646:689	significantly greater mechanical performance	646:689	Obtained results clearly demonstrated that the NF-reinforced composites had significantly greater mechanical performance than the RF-reinforced counterparts.
27339322	8	9	from	trend	1098:1102	arg1	temperature					1128:1138	the glass transition temperature	1107:1138	the glass transition temperature of amylopectin-rich domains in composites	1107:1180	Dynamic mechanical analysis (DMA) revealed an elevational trend in the glass transition temperature of amylopectin-rich domains in composites.
27339322	0	10	theme	composites	97:106	arg1	performance					69:79	linear and non-linear mechanical performance	36:79	linear and non-linear mechanical performance of starch-based composites	36:106	Effect of cellulosic fiber scale on linear and non-linear mechanical performance of starch-based composites.
27339322	0	11	from	Effect	0:5	arg1	performance					69:79	linear and non-linear mechanical performance	36:79	linear and non-linear mechanical performance of starch-based composites	36:106	Effect of cellulosic fiber scale on linear and non-linear mechanical performance of starch-based composites.
27339322	5	12	theme	greater	660:666	arg1	performance					679:689	significantly greater mechanical performance	646:689	significantly greater mechanical performance	646:689	Obtained results clearly demonstrated that the NF-reinforced composites had significantly greater mechanical performance than the RF-reinforced counterparts.
27339322	0	13	theme	starch-based	84:95	arg1	composites					97:106	starch-based composites	84:106	starch-based composites	84:106	Effect of cellulosic fiber scale on linear and non-linear mechanical performance of starch-based composites.
27339322	6	14	theme	fiber/matrix	891:902	arg1	interaction					904:914	strong fiber/matrix interaction	884:914	strong fiber/matrix interaction	884:914	This was attributed to the high aspect ratio and nano dimension of the reinforcing agents, as well as their compatibility with the TPS matrix, resulting in strong fiber/matrix interaction.
27339322	4	15	from	effects	457:463	arg1	performance					533:543	linear and non-linear mechanical performance	500:543	linear and non-linear mechanical performance of fabricated materials	500:567	The aim of study was to investigate the effects of filler dimension and loading on linear and non-linear mechanical performance of fabricated materials.
27339322	4	16	theme	materials	559:567	arg1	performance					533:543	linear and non-linear mechanical performance	500:543	linear and non-linear mechanical performance of fabricated materials	500:567	The aim of study was to investigate the effects of filler dimension and loading on linear and non-linear mechanical performance of fabricated materials.
27339322	3	17	theme	fibers	330:335	arg1	films					358:362	Raw fibers (RFs) reinforced TPS films	326:362	Raw fibers (RFs) reinforced TPS films	326:362	Raw fibers (RFs) reinforced TPS films were prepared with the same contents and conditions.
27339322	6	18	theme	strong	884:889	arg1	interaction					904:914	strong fiber/matrix interaction	884:914	strong fiber/matrix interaction	884:914	This was attributed to the high aspect ratio and nano dimension of the reinforcing agents, as well as their compatibility with the TPS matrix, resulting in strong fiber/matrix interaction.
27339322	6	19	theme	agents	811:816	arg1	ratio					767:771	the high aspect ratio	751:771	the high aspect ratio	751:771	This was attributed to the high aspect ratio and nano dimension of the reinforcing agents, as well as their compatibility with the TPS matrix, resulting in strong fiber/matrix interaction.
27339322	6	19	theme	agents	811:816	arg1	dimension					782:790	nano dimension	777:790	nano dimension	777:790	This was attributed to the high aspect ratio and nano dimension of the reinforcing agents, as well as their compatibility with the TPS matrix, resulting in strong fiber/matrix interaction.
27339322	4	20	theme	fabricated	548:557	arg1	materials					559:567	fabricated materials	548:567	fabricated materials	548:567	The aim of study was to investigate the effects of filler dimension and loading on linear and non-linear mechanical performance of fabricated materials.
27339322	6	21	theme	reinforcing	799:809	arg1	agents					811:816	the reinforcing agents	795:816	the reinforcing agents	795:816	This was attributed to the high aspect ratio and nano dimension of the reinforcing agents, as well as their compatibility with the TPS matrix, resulting in strong fiber/matrix interaction.
27339322	8	22	theme	amylopectin-rich	1143:1158	arg1	domains					1160:1166	amylopectin-rich domains	1143:1166	amylopectin-rich domains in composites	1143:1180	Dynamic mechanical analysis (DMA) revealed an elevational trend in the glass transition temperature of amylopectin-rich domains in composites.
27339322	8	23	theme	domains	1160:1166	arg1	temperature					1128:1138	the glass transition temperature	1107:1138	the glass transition temperature of amylopectin-rich domains in composites	1107:1180	Dynamic mechanical analysis (DMA) revealed an elevational trend in the glass transition temperature of amylopectin-rich domains in composites.
27339322	5	24	theme	NF-reinforced	617:629	arg1	composites					631:640	the NF-reinforced composites	613:640	the NF-reinforced composites	613:640	Obtained results clearly demonstrated that the NF-reinforced composites had significantly greater mechanical performance than the RF-reinforced counterparts.
27339322	9	25	theme	%	1278:1278	arg1	NF					1280:1281	8% NF	1277:1281	8% NF	1277:1281	The most eminent record was +18.5°C shift in temperature position of the film reinforced with 8% NF.
27339322	3	26	theme	TPS	354:356	arg1	films					358:362	Raw fibers (RFs) reinforced TPS films	326:362	Raw fibers (RFs) reinforced TPS films	326:362	Raw fibers (RFs) reinforced TPS films were prepared with the same contents and conditions.
27339322	6	27	with	agents	811:816	arg1	matrix					863:868	the TPS matrix	855:868	the TPS matrix	855:868	This was attributed to the high aspect ratio and nano dimension of the reinforcing agents, as well as their compatibility with the TPS matrix, resulting in strong fiber/matrix interaction.
27339322	4	28	theme	non-linear	511:520	arg1	performance					533:543	linear and non-linear mechanical performance	500:543	linear and non-linear mechanical performance of fabricated materials	500:567	The aim of study was to investigate the effects of filler dimension and loading on linear and non-linear mechanical performance of fabricated materials.
27339322	1	29	theme	glycerol	176:183	arg1	matrices					224:231	glycerol plasticized thermoplastic starch (TPS) matrices	176:231	glycerol plasticized thermoplastic starch (TPS) matrices with varying contents (0-10wt%)	176:263	Cellulosic nanofibers (NFs) from kenaf bast were used to reinforce glycerol plasticized thermoplastic starch (TPS) matrices with varying contents (0-10wt%).
27339322	0	30	theme	fiber	21:25	arg1	scale					27:31	cellulosic fiber scale	10:31	cellulosic fiber scale	10:31	Effect of cellulosic fiber scale on linear and non-linear mechanical performance of starch-based composites.
27339322	6	31	theme	high	755:758	arg1	ratio					767:771	the high aspect ratio	751:771	the high aspect ratio	751:771	This was attributed to the high aspect ratio and nano dimension of the reinforcing agents, as well as their compatibility with the TPS matrix, resulting in strong fiber/matrix interaction.
27339322	8	32	theme	mechanical	1048:1057	arg1	analysis					1059:1066	Dynamic mechanical analysis	1040:1066	Dynamic mechanical analysis (DMA)	1040:1072	Dynamic mechanical analysis (DMA) revealed an elevational trend in the glass transition temperature of amylopectin-rich domains in composites.
27339322	8	32	theme	mechanical	1048:1057	arg1	DMA					1069:1071	DMA	1069:1071	DMA	1069:1071	Dynamic mechanical analysis (DMA) revealed an elevational trend in the glass transition temperature of amylopectin-rich domains in composites.
27339322	7	33	theme	Young	938:942	arg1	modulus					946:952	Young's modulus	938:952	Young's modulus	938:952	Tensile strength and Young's modulus increased by 313% and 343%, respectively, with increasing NF content from 0 to 10wt%.
27339322	5	34	theme	Obtained	570:577	arg1	results					579:585	Obtained results	570:585	Obtained results	570:585	Obtained results clearly demonstrated that the NF-reinforced composites had significantly greater mechanical performance than the RF-reinforced counterparts.
27339322	1	35	theme	plasticized	185:195	arg1	matrices					224:231	glycerol plasticized thermoplastic starch (TPS) matrices	176:231	glycerol plasticized thermoplastic starch (TPS) matrices with varying contents (0-10wt%)	176:263	Cellulosic nanofibers (NFs) from kenaf bast were used to reinforce glycerol plasticized thermoplastic starch (TPS) matrices with varying contents (0-10wt%).
27339322	0	36	theme	cellulosic	10:19	arg1	scale					27:31	cellulosic fiber scale	10:31	cellulosic fiber scale	10:31	Effect of cellulosic fiber scale on linear and non-linear mechanical performance of starch-based composites.
27339322	8	37	theme	glass	1111:1115	arg1	transition					1117:1126	the glass transition	1107:1126	the glass transition temperature of amylopectin-rich domains in composites	1107:1180	Dynamic mechanical analysis (DMA) revealed an elevational trend in the glass transition temperature of amylopectin-rich domains in composites.
27339322	8	38	theme	Dynamic	1040:1046	arg1	analysis					1059:1066	Dynamic mechanical analysis	1040:1066	Dynamic mechanical analysis (DMA)	1040:1072	Dynamic mechanical analysis (DMA) revealed an elevational trend in the glass transition temperature of amylopectin-rich domains in composites.
27339322	8	38	theme	Dynamic	1040:1046	arg1	DMA					1069:1071	DMA	1069:1071	DMA	1069:1071	Dynamic mechanical analysis (DMA) revealed an elevational trend in the glass transition temperature of amylopectin-rich domains in composites.
27339322	1	39	theme	Cellulosic	109:118	arg1	NFs					132:134	NFs	132:134	NFs	132:134	Cellulosic nanofibers (NFs) from kenaf bast were used to reinforce glycerol plasticized thermoplastic starch (TPS) matrices with varying contents (0-10wt%).
27339322	1	39	theme	Cellulosic	109:118	arg1	nanofibers					120:129	Cellulosic nanofibers	109:129	Cellulosic nanofibers (NFs) from kenaf bast	109:151	Cellulosic nanofibers (NFs) from kenaf bast were used to reinforce glycerol plasticized thermoplastic starch (TPS) matrices with varying contents (0-10wt%).
27339322	1	40	theme	thermoplastic	197:209	arg1	matrices					224:231	glycerol plasticized thermoplastic starch (TPS) matrices	176:231	glycerol plasticized thermoplastic starch (TPS) matrices with varying contents (0-10wt%)	176:263	Cellulosic nanofibers (NFs) from kenaf bast were used to reinforce glycerol plasticized thermoplastic starch (TPS) matrices with varying contents (0-10wt%).
27339322	4	41	theme	mechanical	522:531	arg1	performance					533:543	linear and non-linear mechanical performance	500:543	linear and non-linear mechanical performance of fabricated materials	500:567	The aim of study was to investigate the effects of filler dimension and loading on linear and non-linear mechanical performance of fabricated materials.
27339322	9	42	from	shift	1219:1223	arg1	position					1240:1247	temperature position	1228:1247	temperature position of the film reinforced with 8% NF	1228:1281	The most eminent record was +18.5°C shift in temperature position of the film reinforced with 8% NF.
27339322	1	43	theme	starch	211:216	arg1	matrices					224:231	glycerol plasticized thermoplastic starch (TPS) matrices	176:231	glycerol plasticized thermoplastic starch (TPS) matrices with varying contents (0-10wt%)	176:263	Cellulosic nanofibers (NFs) from kenaf bast were used to reinforce glycerol plasticized thermoplastic starch (TPS) matrices with varying contents (0-10wt%).
27339322	0	44	theme	scale	27:31	arg1	Effect					0:5	Effect	0:5	Effect of cellulosic fiber scale on linear and non-linear mechanical performance of starch-based composites.	0:107	Effect of cellulosic fiber scale on linear and non-linear mechanical performance of starch-based composites.
27339322	4	45	theme	loading	489:495	arg1	effects					457:463	the effects	453:463	the effects of filler dimension and loading on linear and non-linear mechanical performance of fabricated materials	453:567	The aim of study was to investigate the effects of filler dimension and loading on linear and non-linear mechanical performance of fabricated materials.
27339322	3	46	theme	same	387:390	arg1	contents					392:399	the same contents	383:399	the same contents	383:399	Raw fibers (RFs) reinforced TPS films were prepared with the same contents and conditions.
27339322	4	47	theme	study	428:432	arg1	aim					421:423	The aim	417:423	The aim of study	417:432	The aim of study was to investigate the effects of filler dimension and loading on linear and non-linear mechanical performance of fabricated materials.
27339322	3	48	theme	reinforced	343:352	arg1	films					358:362	Raw fibers (RFs) reinforced TPS films	326:362	Raw fibers (RFs) reinforced TPS films	326:362	Raw fibers (RFs) reinforced TPS films were prepared with the same contents and conditions.
27339322	6	49	theme	nano	777:780	arg1	dimension					782:790	nano dimension	777:790	nano dimension	777:790	This was attributed to the high aspect ratio and nano dimension of the reinforcing agents, as well as their compatibility with the TPS matrix, resulting in strong fiber/matrix interaction.
27339322	6	50	with	compatibility	836:848	arg1	matrix					863:868	the TPS matrix	855:868	the TPS matrix	855:868	This was attributed to the high aspect ratio and nano dimension of the reinforcing agents, as well as their compatibility with the TPS matrix, resulting in strong fiber/matrix interaction.
27339322	8	51	theme	transition	1117:1126	arg1	temperature					1128:1138	the glass transition temperature	1107:1138	the glass transition temperature of amylopectin-rich domains in composites	1107:1180	Dynamic mechanical analysis (DMA) revealed an elevational trend in the glass transition temperature of amylopectin-rich domains in composites.
27339322	10	52	from	dispersion	1315:1324	arg1	matrix					1347:1352	the matrix	1343:1352	the matrix	1343:1352	This finding implied efficient dispersion of nanofibers in the matrix and their ability to form a network and restrict mobility of the system.
27339322	3	53	theme	Raw	326:328	arg1	RFs					338:340	RFs	338:340	RFs	338:340	Raw fibers (RFs) reinforced TPS films were prepared with the same contents and conditions.
27339322	3	53	theme	Raw	326:328	arg1	fibers					330:335	Raw fibers	326:335	Raw fibers (RFs) reinforced TPS films	326:362	Raw fibers (RFs) reinforced TPS films were prepared with the same contents and conditions.
27339322	0	54	theme	linear	36:41	arg1	performance					69:79	linear and non-linear mechanical performance	36:79	linear and non-linear mechanical performance of starch-based composites	36:106	Effect of cellulosic fiber scale on linear and non-linear mechanical performance of starch-based composites.
27339322	4	55	theme	linear	500:505	arg1	performance					533:543	linear and non-linear mechanical performance	500:543	linear and non-linear mechanical performance of fabricated materials	500:567	The aim of study was to investigate the effects of filler dimension and loading on linear and non-linear mechanical performance of fabricated materials.
27339322	8	56	from	domains	1160:1166	arg1	composites					1171:1180	composites	1171:1180	composites	1171:1180	Dynamic mechanical analysis (DMA) revealed an elevational trend in the glass transition temperature of amylopectin-rich domains in composites.
27339322	8	57	from	temperature	1128:1138	arg1	composites					1171:1180	composites	1171:1180	composites	1171:1180	Dynamic mechanical analysis (DMA) revealed an elevational trend in the glass transition temperature of amylopectin-rich domains in composites.
27339322	6	58	theme	TPS	859:861	arg1	matrix					863:868	the TPS matrix	855:868	the TPS matrix	855:868	This was attributed to the high aspect ratio and nano dimension of the reinforcing agents, as well as their compatibility with the TPS matrix, resulting in strong fiber/matrix interaction.
27339322	7	59	theme	Tensile	917:923	arg1	strength					925:932	Tensile strength	917:932	Tensile strength	917:932	Tensile strength and Young's modulus increased by 313% and 343%, respectively, with increasing NF content from 0 to 10wt%.
27339322	9	60	theme	eminent	1192:1198	arg1	record					1200:1205	The most eminent record	1183:1205	The most eminent record	1183:1205	The most eminent record was +18.5°C shift in temperature position of the film reinforced with 8% NF.
27339322	9	60	theme	eminent	1192:1198	arg1	shift					1219:1223	+18.5°C shift	1211:1223	+18.5°C shift	1211:1223	The most eminent record was +18.5°C shift in temperature position of the film reinforced with 8% NF.
27339322	7	61	theme	NF	1012:1013	arg1	content					1015:1021	NF content	1012:1021	NF content	1012:1021	Tensile strength and Young's modulus increased by 313% and 343%, respectively, with increasing NF content from 0 to 10wt%.
27339322	6	62	theme	compatibility	836:848	arg1	ratio					767:771	the high aspect ratio	751:771	the high aspect ratio	751:771	This was attributed to the high aspect ratio and nano dimension of the reinforcing agents, as well as their compatibility with the TPS matrix, resulting in strong fiber/matrix interaction.
27339322	6	62	theme	compatibility	836:848	arg1	dimension					782:790	nano dimension	777:790	nano dimension	777:790	This was attributed to the high aspect ratio and nano dimension of the reinforcing agents, as well as their compatibility with the TPS matrix, resulting in strong fiber/matrix interaction.
27339322	6	63	theme	aspect	760:765	arg1	ratio					767:771	the high aspect ratio	751:771	the high aspect ratio	751:771	This was attributed to the high aspect ratio and nano dimension of the reinforcing agents, as well as their compatibility with the TPS matrix, resulting in strong fiber/matrix interaction.
27339322	1	64	theme	TPS	219:221	arg1	matrices					224:231	glycerol plasticized thermoplastic starch (TPS) matrices	176:231	glycerol plasticized thermoplastic starch (TPS) matrices with varying contents (0-10wt%)	176:263	Cellulosic nanofibers (NFs) from kenaf bast were used to reinforce glycerol plasticized thermoplastic starch (TPS) matrices with varying contents (0-10wt%).
27339322	5	65	theme	RF-reinforced	700:712	arg1	counterparts					714:725	the RF-reinforced counterparts	696:725	the RF-reinforced counterparts	696:725	Obtained results clearly demonstrated that the NF-reinforced composites had significantly greater mechanical performance than the RF-reinforced counterparts.
27339322	4	66	theme	filler	468:473	arg1	dimension					475:483	filler dimension	468:483	filler dimension	468:483	The aim of study was to investigate the effects of filler dimension and loading on linear and non-linear mechanical performance of fabricated materials.
27339322	10	67	theme	efficient	1305:1313	arg1	dispersion					1315:1324	efficient dispersion	1305:1324	efficient dispersion of nanofibers in the matrix	1305:1352	This finding implied efficient dispersion of nanofibers in the matrix and their ability to form a network and restrict mobility of the system.
27339322	1	68	with	matrices	224:231	arg1	contents					246:253	varying contents	238:253	varying contents (0-10wt%)	238:263	Cellulosic nanofibers (NFs) from kenaf bast were used to reinforce glycerol plasticized thermoplastic starch (TPS) matrices with varying contents (0-10wt%).
27339322	1	68	with	matrices	224:231	arg1	%					262:262	0-10wt%	256:262	0-10wt%	256:262	Cellulosic nanofibers (NFs) from kenaf bast were used to reinforce glycerol plasticized thermoplastic starch (TPS) matrices with varying contents (0-10wt%).
27339322	2	69	theme	casting/evaporation	298:316	arg1	method					318:323	casting/evaporation method	298:323	casting/evaporation method	298:323	The composites were prepared by casting/evaporation method.
27339322	8	70	theme	elevational	1086:1096	arg1	trend					1098:1102	an elevational trend	1083:1102	an elevational trend in the glass transition temperature of amylopectin-rich domains in composites	1083:1180	Dynamic mechanical analysis (DMA) revealed an elevational trend in the glass transition temperature of amylopectin-rich domains in composites.
27339322	0	71	theme	mechanical	58:67	arg1	performance					69:79	linear and non-linear mechanical performance	36:79	linear and non-linear mechanical performance of starch-based composites	36:106	Effect of cellulosic fiber scale on linear and non-linear mechanical performance of starch-based composites.
27339322	10	72	theme	nanofibers	1329:1338	arg1	ability					1364:1370	their ability to form a network and restrict mobility of the system	1358:1424	their ability to form a network and restrict mobility of the system	1358:1424	This finding implied efficient dispersion of nanofibers in the matrix and their ability to form a network and restrict mobility of the system.
27339322	10	72	theme	nanofibers	1329:1338	arg1	dispersion					1315:1324	efficient dispersion	1305:1324	efficient dispersion of nanofibers in the matrix	1305:1352	This finding implied efficient dispersion of nanofibers in the matrix and their ability to form a network and restrict mobility of the system.
27339322	9	73	theme	temperature	1228:1238	arg1	position					1240:1247	temperature position	1228:1247	temperature position of the film reinforced with 8% NF	1228:1281	The most eminent record was +18.5°C shift in temperature position of the film reinforced with 8% NF.
27339322	0	74	theme	non-linear	47:56	arg1	performance					69:79	linear and non-linear mechanical performance	36:79	linear and non-linear mechanical performance of starch-based composites	36:106	Effect of cellulosic fiber scale on linear and non-linear mechanical performance of starch-based composites.
27339322	8	75	from	composites	1171:1180	arg1	temperature					1128:1138	the glass transition temperature	1107:1138	the glass transition temperature of amylopectin-rich domains in composites	1107:1180	Dynamic mechanical analysis (DMA) revealed an elevational trend in the glass transition temperature of amylopectin-rich domains in composites.
27339322	1	76	used	used	158:161	arg2	nanofibers					120:129	Cellulosic nanofibers	109:129	Cellulosic nanofibers (NFs) from kenaf bast	109:151	Cellulosic nanofibers (NFs) from kenaf bast were used to reinforce glycerol plasticized thermoplastic starch (TPS) matrices with varying contents (0-10wt%).
27339322	1	76	used	used	158:161	arg2	NFs					132:134	NFs	132:134	NFs	132:134	Cellulosic nanofibers (NFs) from kenaf bast were used to reinforce glycerol plasticized thermoplastic starch (TPS) matrices with varying contents (0-10wt%).
27339322	1	77	theme	kenaf	142:146	arg1	bast					148:151	kenaf bast	142:151	kenaf bast	142:151	Cellulosic nanofibers (NFs) from kenaf bast were used to reinforce glycerol plasticized thermoplastic starch (TPS) matrices with varying contents (0-10wt%).
27339322	5	78	contain	had	642:644	arg2	performance					679:689	significantly greater mechanical performance	646:689	significantly greater mechanical performance	646:689	Obtained results clearly demonstrated that the NF-reinforced composites had significantly greater mechanical performance than the RF-reinforced counterparts.
27339322	5	78	contain	had	642:644	arg1	composites					631:640	the NF-reinforced composites	613:640	the NF-reinforced composites	613:640	Obtained results clearly demonstrated that the NF-reinforced composites had significantly greater mechanical performance than the RF-reinforced counterparts.
28237996	5	0	theme	lactic	558:563	arg1	acid					565:568	lactic acid	558:568	lactic acid (73.34 mM)	558:579	The metabolites in chemostat culture were lactic acid (73.34 mM), acetic acid (7.69 mM), and mannitol (9.93 mM).
28237996	5	0	theme	lactic	558:563	arg1	mM					577:578	73.34 mM	571:578	73.34 mM	571:578	The metabolites in chemostat culture were lactic acid (73.34 mM), acetic acid (7.69 mM), and mannitol (9.93 mM).
28237996	5	0	theme	lactic	558:563	arg1	metabolites					520:530	The metabolites	516:530	The metabolites in chemostat culture	516:551	The metabolites in chemostat culture were lactic acid (73.34 mM), acetic acid (7.69 mM), and mannitol (9.93 mM).
28237996	1	1	theme	Leuconostoc	166:176	arg1	mesenteroides					178:190	Leuconostoc mesenteroides ATCC 8293	166:200	Leuconostoc mesenteroides ATCC 8293	166:200	The cellular composition and metabolic compounds of Leuconostoc mesenteroides ATCC 8293 were analyzed after cultivation in an anaerobic chemostat.
28237996	4	2	theme	>	507:507	arg1	S					513:513	C > O > N > H > S	497:513	C > O > N > H > S	497:513	Elements were in the order of C > O > N > H > S.
28237996	1	3	theme	mesenteroides	178:190	arg1	composition					127:137	cellular composition	118:137	cellular composition	118:137	The cellular composition and metabolic compounds of Leuconostoc mesenteroides ATCC 8293 were analyzed after cultivation in an anaerobic chemostat.
28237996	1	3	theme	mesenteroides	178:190	arg1	compounds					153:161	metabolic compounds	143:161	metabolic compounds	143:161	The cellular composition and metabolic compounds of Leuconostoc mesenteroides ATCC 8293 were analyzed after cultivation in an anaerobic chemostat.
28237996	4	4	theme	N	505:505	arg1	S					513:513	C > O > N > H > S	497:513	C > O > N > H > S	497:513	Elements were in the order of C > O > N > H > S.
28237996	5	5	from	metabolites	520:530	arg1	culture					545:551	chemostat culture	535:551	chemostat culture	535:551	The metabolites in chemostat culture were lactic acid (73.34 mM), acetic acid (7.69 mM), and mannitol (9.93 mM).
28237996	4	6	theme	>	511:511	arg1	S					513:513	C > O > N > H > S	497:513	C > O > N > H > S	497:513	Elements were in the order of C > O > N > H > S.
28237996	2	7	dep	%	348:348	arg1	DNA					350:352	DNA	350:352	2.9% DNA	345:352	The macromolecular composition was 24.4% polysaccharide, 29.7% protein, 7.9% lipid, 2.9% DNA, and 7.4% RNA.
28237996	6	8	theme	first	650:654	arg1	view					656:659	a first view	648:659	a first view of the cellular composition of L. mesenteroides for use in metabolic flux analysis	648:742	These data provide a first view of the cellular composition of L. mesenteroides for use in metabolic flux analysis.
28237996	3	9	theme	leucine	458:464	arg1	amounts					411:417	large amounts	405:417	large amounts of lysine, glutamic acid, alanine, and leucine	405:464	Its amino acid composition included large amounts of lysine, glutamic acid, alanine, and leucine.
28237996	3	9	theme	leucine	458:464	arg1	alanine					445:451	alanine	445:451	alanine	445:451	Its amino acid composition included large amounts of lysine, glutamic acid, alanine, and leucine.
28237996	3	9	theme	leucine	458:464	arg1	lysine					422:427	lysine	422:427	lysine	422:427	Its amino acid composition included large amounts of lysine, glutamic acid, alanine, and leucine.
28237996	3	9	theme	leucine	458:464	arg1	leucine					458:464	leucine	458:464	leucine	458:464	Its amino acid composition included large amounts of lysine, glutamic acid, alanine, and leucine.
28237996	3	9	theme	leucine	458:464	arg1	acid					439:442	glutamic acid	430:442	glutamic acid	430:442	Its amino acid composition included large amounts of lysine, glutamic acid, alanine, and leucine.
28237996	3	10	theme	alanine	445:451	arg1	amounts					411:417	large amounts	405:417	large amounts of lysine, glutamic acid, alanine, and leucine	405:464	Its amino acid composition included large amounts of lysine, glutamic acid, alanine, and leucine.
28237996	3	10	theme	alanine	445:451	arg1	alanine					445:451	alanine	445:451	alanine	445:451	Its amino acid composition included large amounts of lysine, glutamic acid, alanine, and leucine.
28237996	3	10	theme	alanine	445:451	arg1	lysine					422:427	lysine	422:427	lysine	422:427	Its amino acid composition included large amounts of lysine, glutamic acid, alanine, and leucine.
28237996	3	10	theme	alanine	445:451	arg1	leucine					458:464	leucine	458:464	leucine	458:464	Its amino acid composition included large amounts of lysine, glutamic acid, alanine, and leucine.
28237996	3	10	theme	alanine	445:451	arg1	acid					439:442	glutamic acid	430:442	glutamic acid	430:442	Its amino acid composition included large amounts of lysine, glutamic acid, alanine, and leucine.
28237996	6	11	theme	flux	730:733	arg1	analysis					735:742	metabolic flux analysis	720:742	metabolic flux analysis	720:742	These data provide a first view of the cellular composition of L. mesenteroides for use in metabolic flux analysis.
28237996	1	12	theme	ATCC	192:195	arg1	8293					197:200	ATCC 8293	192:200	Leuconostoc mesenteroides ATCC 8293	166:200	The cellular composition and metabolic compounds of Leuconostoc mesenteroides ATCC 8293 were analyzed after cultivation in an anaerobic chemostat.
28237996	3	13	theme	amino	373:377	arg1	composition					384:394	Its amino acid composition	369:394	Its amino acid composition	369:394	Its amino acid composition included large amounts of lysine, glutamic acid, alanine, and leucine.
28237996	6	14	theme	metabolic	720:728	arg1	analysis					735:742	metabolic flux analysis	720:742	metabolic flux analysis	720:742	These data provide a first view of the cellular composition of L. mesenteroides for use in metabolic flux analysis.
28237996	5	15	theme	chemostat	535:543	arg1	culture					545:551	chemostat culture	535:551	chemostat culture	535:551	The metabolites in chemostat culture were lactic acid (73.34 mM), acetic acid (7.69 mM), and mannitol (9.93 mM).
28237996	1	16	dep	composition	127:137	arg1	The					114:116	The	114:116	The	114:116	The cellular composition and metabolic compounds of Leuconostoc mesenteroides ATCC 8293 were analyzed after cultivation in an anaerobic chemostat.
28237996	4	17	theme	>	499:499	arg1	S					513:513	C > O > N > H > S	497:513	C > O > N > H > S	497:513	Elements were in the order of C > O > N > H > S.
28237996	2	18	dep	%	336:336	arg1	lipid					338:342	lipid	338:342	7.9% lipid	333:342	The macromolecular composition was 24.4% polysaccharide, 29.7% protein, 7.9% lipid, 2.9% DNA, and 7.4% RNA.
28237996	3	19	theme	large	405:409	arg1	amounts					411:417	large amounts	405:417	large amounts of lysine, glutamic acid, alanine, and leucine	405:464	Its amino acid composition included large amounts of lysine, glutamic acid, alanine, and leucine.
28237996	3	19	theme	large	405:409	arg1	alanine					445:451	alanine	445:451	alanine	445:451	Its amino acid composition included large amounts of lysine, glutamic acid, alanine, and leucine.
28237996	3	19	theme	large	405:409	arg1	lysine					422:427	lysine	422:427	lysine	422:427	Its amino acid composition included large amounts of lysine, glutamic acid, alanine, and leucine.
28237996	3	19	theme	large	405:409	arg1	leucine					458:464	leucine	458:464	leucine	458:464	Its amino acid composition included large amounts of lysine, glutamic acid, alanine, and leucine.
28237996	3	19	theme	large	405:409	arg1	acid					439:442	glutamic acid	430:442	glutamic acid	430:442	Its amino acid composition included large amounts of lysine, glutamic acid, alanine, and leucine.
28237996	6	20	from	use	713:715	arg1	analysis					735:742	metabolic flux analysis	720:742	metabolic flux analysis	720:742	These data provide a first view of the cellular composition of L. mesenteroides for use in metabolic flux analysis.
28237996	4	21	theme	C	497:497	arg1	S					513:513	C > O > N > H > S	497:513	C > O > N > H > S	497:513	Elements were in the order of C > O > N > H > S.
28237996	3	22	theme	glutamic	430:437	arg1	acid					439:442	glutamic acid	430:442	glutamic acid	430:442	Its amino acid composition included large amounts of lysine, glutamic acid, alanine, and leucine.
28237996	1	23	dep	mesenteroides	178:190	arg1	8293					197:200	ATCC 8293	192:200	Leuconostoc mesenteroides ATCC 8293	166:200	The cellular composition and metabolic compounds of Leuconostoc mesenteroides ATCC 8293 were analyzed after cultivation in an anaerobic chemostat.
28237996	4	24	theme	>	503:503	arg1	S					513:513	C > O > N > H > S	497:513	C > O > N > H > S	497:513	Elements were in the order of C > O > N > H > S.
28237996	3	25	theme	acid	439:442	arg1	amounts					411:417	large amounts	405:417	large amounts of lysine, glutamic acid, alanine, and leucine	405:464	Its amino acid composition included large amounts of lysine, glutamic acid, alanine, and leucine.
28237996	3	25	theme	acid	439:442	arg1	alanine					445:451	alanine	445:451	alanine	445:451	Its amino acid composition included large amounts of lysine, glutamic acid, alanine, and leucine.
28237996	3	25	theme	acid	439:442	arg1	lysine					422:427	lysine	422:427	lysine	422:427	Its amino acid composition included large amounts of lysine, glutamic acid, alanine, and leucine.
28237996	3	25	theme	acid	439:442	arg1	leucine					458:464	leucine	458:464	leucine	458:464	Its amino acid composition included large amounts of lysine, glutamic acid, alanine, and leucine.
28237996	3	25	theme	acid	439:442	arg1	acid					439:442	glutamic acid	430:442	glutamic acid	430:442	Its amino acid composition included large amounts of lysine, glutamic acid, alanine, and leucine.
28237996	3	26	theme	acid	379:382	arg1	composition					384:394	Its amino acid composition	369:394	Its amino acid composition	369:394	Its amino acid composition included large amounts of lysine, glutamic acid, alanine, and leucine.
28237996	4	27	theme	O	501:501	arg1	S					513:513	C > O > N > H > S	497:513	C > O > N > H > S	497:513	Elements were in the order of C > O > N > H > S.
28237996	0	28	dep	mesenteroides	65:77	arg1	ATCC					79:82	ATCC 8293	79:87	Leuconostoc mesenteroides ATCC 8293	53:87	Macromolecular and Elemental Composition Analyses of Leuconostoc mesenteroides ATCC 8293 Cultured in a Chemostat.
28237996	3	29	theme	lysine	422:427	arg1	amounts					411:417	large amounts	405:417	large amounts of lysine, glutamic acid, alanine, and leucine	405:464	Its amino acid composition included large amounts of lysine, glutamic acid, alanine, and leucine.
28237996	3	29	theme	lysine	422:427	arg1	alanine					445:451	alanine	445:451	alanine	445:451	Its amino acid composition included large amounts of lysine, glutamic acid, alanine, and leucine.
28237996	3	29	theme	lysine	422:427	arg1	lysine					422:427	lysine	422:427	lysine	422:427	Its amino acid composition included large amounts of lysine, glutamic acid, alanine, and leucine.
28237996	3	29	theme	lysine	422:427	arg1	leucine					458:464	leucine	458:464	leucine	458:464	Its amino acid composition included large amounts of lysine, glutamic acid, alanine, and leucine.
28237996	3	29	theme	lysine	422:427	arg1	acid					439:442	glutamic acid	430:442	glutamic acid	430:442	Its amino acid composition included large amounts of lysine, glutamic acid, alanine, and leucine.
28237996	0	30	theme	Elemental	19:27	arg1	Composition					29:39	Elemental Composition	19:39	Elemental Composition	19:39	Macromolecular and Elemental Composition Analyses of Leuconostoc mesenteroides ATCC 8293 Cultured in a Chemostat.
28237996	6	31	theme	mesenteroides	695:707	arg1	composition					677:687	the cellular composition	664:687	the cellular composition of L. mesenteroides	664:707	These data provide a first view of the cellular composition of L. mesenteroides for use in metabolic flux analysis.
28237996	0	32	theme	mesenteroides	65:77	arg1	Macromolecular					0:13	Macromolecular	0:13	Macromolecular	0:13	Macromolecular and Elemental Composition Analyses of Leuconostoc mesenteroides ATCC 8293 Cultured in a Chemostat.
28237996	6	33	theme	L.	692:693	arg1	mesenteroides					695:707	L. mesenteroides	692:707	L. mesenteroides	692:707	These data provide a first view of the cellular composition of L. mesenteroides for use in metabolic flux analysis.
28237996	1	34	theme	cellular	118:125	arg1	composition					127:137	cellular composition	118:137	cellular composition	118:137	The cellular composition and metabolic compounds of Leuconostoc mesenteroides ATCC 8293 were analyzed after cultivation in an anaerobic chemostat.
28237996	0	35	theme	Leuconostoc	53:63	arg1	mesenteroides					65:77	Leuconostoc mesenteroides	53:77	Leuconostoc mesenteroides ATCC 8293	53:87	Macromolecular and Elemental Composition Analyses of Leuconostoc mesenteroides ATCC 8293 Cultured in a Chemostat.
28237996	2	36	dep	%	322:322	arg1	protein					324:330	protein	324:330	29.7% protein	318:330	The macromolecular composition was 24.4% polysaccharide, 29.7% protein, 7.9% lipid, 2.9% DNA, and 7.4% RNA.
28237996	0	37	dep	Macromolecular	0:13	arg1	Analyses					41:48	Analyses	41:48	Analyses	41:48	Macromolecular and Elemental Composition Analyses of Leuconostoc mesenteroides ATCC 8293 Cultured in a Chemostat.
28237996	6	38	theme	composition	677:687	arg1	view					656:659	a first view	648:659	a first view of the cellular composition of L. mesenteroides for use in metabolic flux analysis	648:742	These data provide a first view of the cellular composition of L. mesenteroides for use in metabolic flux analysis.
28237996	2	39	dep	%	362:362	arg1	RNA					364:366	RNA	364:366	7.4% RNA	359:366	The macromolecular composition was 24.4% polysaccharide, 29.7% protein, 7.9% lipid, 2.9% DNA, and 7.4% RNA.
28237996	1	40	theme	anaerobic	240:248	arg1	chemostat					250:258	an anaerobic chemostat	237:258	an anaerobic chemostat	237:258	The cellular composition and metabolic compounds of Leuconostoc mesenteroides ATCC 8293 were analyzed after cultivation in an anaerobic chemostat.
28237996	5	41	theme	acetic	582:587	arg1	acid					589:592	acetic acid	582:592	acetic acid (7.69 mM)	582:602	The metabolites in chemostat culture were lactic acid (73.34 mM), acetic acid (7.69 mM), and mannitol (9.93 mM).
28237996	5	41	theme	acetic	582:587	arg1	mM					600:601	7.69 mM	595:601	7.69 mM	595:601	The metabolites in chemostat culture were lactic acid (73.34 mM), acetic acid (7.69 mM), and mannitol (9.93 mM).
28237996	6	42	theme	cellular	668:675	arg1	composition					677:687	the cellular composition	664:687	the cellular composition of L. mesenteroides	664:707	These data provide a first view of the cellular composition of L. mesenteroides for use in metabolic flux analysis.
28237996	1	43	theme	metabolic	143:151	arg1	compounds					153:161	metabolic compounds	143:161	metabolic compounds	143:161	The cellular composition and metabolic compounds of Leuconostoc mesenteroides ATCC 8293 were analyzed after cultivation in an anaerobic chemostat.
28237996	4	44	theme	S	513:513	arg1	order					488:492	the order	484:492	the order of C > O > N > H > S	484:513	Elements were in the order of C > O > N > H > S.
28237996	4	45	theme	H	509:509	arg1	S					513:513	C > O > N > H > S	497:513	C > O > N > H > S	497:513	Elements were in the order of C > O > N > H > S.
28237996	5	46	theme	9.93	619:622	arg1	mannitol					609:616	mannitol	609:616	mannitol (9.93 mM)	609:626	The metabolites in chemostat culture were lactic acid (73.34 mM), acetic acid (7.69 mM), and mannitol (9.93 mM).
28237996	5	46	theme	9.93	619:622	arg1	mM					624:625	9.93 mM	619:625	9.93 mM	619:625	The metabolites in chemostat culture were lactic acid (73.34 mM), acetic acid (7.69 mM), and mannitol (9.93 mM).
28237996	2	47	theme	macromolecular	265:278	arg1	composition					280:290	The macromolecular composition	261:290	The macromolecular composition	261:290	The macromolecular composition was 24.4% polysaccharide, 29.7% protein, 7.9% lipid, 2.9% DNA, and 7.4% RNA.
28237996	2	47	theme	macromolecular	265:278	arg1	%					300:300	24.4%	296:300	24.4% polysaccharide	296:315	The macromolecular composition was 24.4% polysaccharide, 29.7% protein, 7.9% lipid, 2.9% DNA, and 7.4% RNA.
28237996	2	48	dep	%	300:300	arg1	polysaccharide					302:315	polysaccharide	302:315	24.4% polysaccharide	296:315	The macromolecular composition was 24.4% polysaccharide, 29.7% protein, 7.9% lipid, 2.9% DNA, and 7.4% RNA.
26525014	5	0	theme	eryngii	661:667	arg1	ability					647:653	the utilization ability	631:653	the utilization ability of P. eryngii to ramie stalk substrate	631:692	Besides, the utilization ability of P. eryngii to ramie stalk substrate was evaluated from the degradation of cellulose, hemicellulose, and lignin in medium and six extracellular enzymes activities during different growth stages were discussed.
26525014	6	1	theme	eryngii	1005:1011	arg1	rates					993:997	cellulose, hemicelluloses, and lignin decomposition rates	941:997	cellulose, hemicelluloses, and lignin decomposition rates of P. eryngii	941:1011	The results showed that a high biological efficiency of 71% was obtained; cellulose, hemicelluloses, and lignin decomposition rates of P. eryngii were 29.2, 26.0, and 51.2%, respectively.
26525014	0	2	theme	stalk	80:84	arg1	degradation					86:96	ramie stalk degradation	74:96	ramie stalk degradation	74:96	Secretome analysis of Pleurotus eryngii reveals enzymatic composition for ramie stalk degradation.
26525014	6	3	theme	%	925:925	arg1	efficiency					909:918	a high biological efficiency	891:918	a high biological efficiency of 71%	891:925	The results showed that a high biological efficiency of 71% was obtained; cellulose, hemicelluloses, and lignin decomposition rates of P. eryngii were 29.2, 26.0, and 51.2%, respectively.
26525014	5	4	theme	extracellular	787:799	arg1	enzymes					801:807	six extracellular enzymes	783:807	six extracellular enzymes activities during different growth stages were discussed	783:864	Besides, the utilization ability of P. eryngii to ramie stalk substrate was evaluated from the degradation of cellulose, hemicellulose, and lignin in medium and six extracellular enzymes activities during different growth stages were discussed.
26525014	8	5	theme	stalk	1292:1296	arg1	media					1298:1302	ramie stalk media	1286:1302	ramie stalk media	1286:1302	In addition, we profiled a global view of the secretome of P. eryngii cultivated in ramie stalk media to understand the mechanism behind lignocellulosic biomass hydrolysis.
26525014	7	6	theme	xylanase	1108:1115	arg1	peaks					1151:1155	carboxymethyl cellulase, xylanase, laccase, and peroxidase activity peaks	1083:1155	carboxymethyl cellulase, xylanase, laccase, and peroxidase activity peaks	1083:1155	Enzyme activity showed that carboxymethyl cellulase, xylanase, laccase, and peroxidase activity peaks appeared at the primordial initiation stage.
26525014	7	7	theme	initiation	1184:1193	arg1	stage					1195:1199	the primordial initiation stage	1169:1199	the primordial initiation stage	1169:1199	Enzyme activity showed that carboxymethyl cellulase, xylanase, laccase, and peroxidase activity peaks appeared at the primordial initiation stage.
26525014	5	8	theme	ramie	672:676	arg1	substrate					684:692	ramie stalk substrate	672:692	ramie stalk substrate	672:692	Besides, the utilization ability of P. eryngii to ramie stalk substrate was evaluated from the degradation of cellulose, hemicellulose, and lignin in medium and six extracellular enzymes activities during different growth stages were discussed.
26525014	10	9	from	helpful	1663:1669	arg1	conclusion					1612:1621	conclusion	1612:1621	conclusion	1612:1621	In conclusion, the information in this report will be helpful to better understand the lignocelluloses degradation mechanisms of P. eryngii.
26525014	6	10	theme	decomposition	979:991	arg1	rates					993:997	cellulose, hemicelluloses, and lignin decomposition rates	941:997	cellulose, hemicelluloses, and lignin decomposition rates of P. eryngii	941:1011	The results showed that a high biological efficiency of 71% was obtained; cellulose, hemicelluloses, and lignin decomposition rates of P. eryngii were 29.2, 26.0, and 51.2%, respectively.
26525014	5	11	theme	hemicellulose	743:755	arg1	degradation					717:727	the degradation	713:727	the degradation of cellulose, hemicellulose, and lignin	713:767	Besides, the utilization ability of P. eryngii to ramie stalk substrate was evaluated from the degradation of cellulose, hemicellulose, and lignin in medium and six extracellular enzymes activities during different growth stages were discussed.
26525014	5	12	theme	stalk	678:682	arg1	substrate					684:692	ramie stalk substrate	672:692	ramie stalk substrate	672:692	Besides, the utilization ability of P. eryngii to ramie stalk substrate was evaluated from the degradation of cellulose, hemicellulose, and lignin in medium and six extracellular enzymes activities during different growth stages were discussed.
26525014	0	13	theme	Secretome	0:8	arg1	analysis					10:17	Secretome analysis	0:17	Secretome analysis of Pleurotus eryngii	0:38	Secretome analysis of Pleurotus eryngii reveals enzymatic composition for ramie stalk degradation.
26525014	4	14	theme	lignocellulose	594:607	arg1	degradation					609:619	lignocellulose degradation	594:619	lignocellulose degradation	594:619	In this report, secretomic analysis was used to search for the new or interesting enzymes for lignocellulose degradation.
26525014	10	15	from	conclusion	1612:1621	arg1	helpful					1663:1669	helpful	1663:1669	helpful	1663:1669	In conclusion, the information in this report will be helpful to better understand the lignocelluloses degradation mechanisms of P. eryngii.
26525014	10	15	from	conclusion	1612:1621	arg1	information					1628:1638	the information	1624:1638	the information in this report	1624:1653	In conclusion, the information in this report will be helpful to better understand the lignocelluloses degradation mechanisms of P. eryngii.
26525014	5	16	theme	utilization	635:645	arg1	ability					647:653	the utilization ability	631:653	the utilization ability of P. eryngii to ramie stalk substrate	631:692	Besides, the utilization ability of P. eryngii to ramie stalk substrate was evaluated from the degradation of cellulose, hemicellulose, and lignin in medium and six extracellular enzymes activities during different growth stages were discussed.
26525014	2	17	theme	lignolytic	276:285	arg1	enzymes					287:293	the individual lignolytic enzymes	261:293	the individual lignolytic enzymes	261:293	In spite of several researches on the individual lignolytic enzymes, a direct deconstruction of lignocellulose by enzyme mixture is not yet possible.
26525014	8	18	theme	global	1229:1234	arg1	view					1236:1239	a global view	1227:1239	a global view of the secretome of P. eryngii cultivated in ramie stalk media to understand the mechanism behind lignocellulosic biomass hydrolysis	1227:1372	In addition, we profiled a global view of the secretome of P. eryngii cultivated in ramie stalk media to understand the mechanism behind lignocellulosic biomass hydrolysis.
26525014	1	19	theme	hydrolytic	158:167	arg1	enzymes					183:189	hydrolytic and oxidative enzymes	158:189	hydrolytic and oxidative enzymes	158:189	Pleurotus eryngii (P. eryngii) can secrete large amount of hydrolytic and oxidative enzymes to degrade lignocellulosic biomass.
26525014	7	20	theme	primordial	1173:1182	arg1	stage					1195:1199	the primordial initiation stage	1169:1199	the primordial initiation stage	1169:1199	Enzyme activity showed that carboxymethyl cellulase, xylanase, laccase, and peroxidase activity peaks appeared at the primordial initiation stage.
26525014	6	21	theme	biological	898:907	arg1	efficiency					909:918	a high biological efficiency	891:918	a high biological efficiency of 71%	891:925	The results showed that a high biological efficiency of 71% was obtained; cellulose, hemicelluloses, and lignin decomposition rates of P. eryngii were 29.2, 26.0, and 51.2%, respectively.
26525014	7	22	theme	carboxymethyl	1083:1095	arg1	cellulase					1097:1105	carboxymethyl cellulase	1083:1105	carboxymethyl cellulase	1083:1105	Enzyme activity showed that carboxymethyl cellulase, xylanase, laccase, and peroxidase activity peaks appeared at the primordial initiation stage.
26525014	2	23	theme	individual	265:274	arg1	enzymes					287:293	the individual lignolytic enzymes	261:293	the individual lignolytic enzymes	261:293	In spite of several researches on the individual lignolytic enzymes, a direct deconstruction of lignocellulose by enzyme mixture is not yet possible.
26525014	3	24	theme	efficient	452:460	arg1	degradation					487:497	efficient in vitro lignocelluloses degradation	452:497	efficient in vitro lignocelluloses degradation	452:497	Identifying more high-performance enzymes or enzyme complexes will lead to efficient in vitro lignocelluloses degradation.
26525014	6	25	theme	high	893:896	arg1	efficiency					909:918	a high biological efficiency	891:918	a high biological efficiency of 71%	891:925	The results showed that a high biological efficiency of 71% was obtained; cellulose, hemicelluloses, and lignin decomposition rates of P. eryngii were 29.2, 26.0, and 51.2%, respectively.
26525014	4	26	used	used	540:543	arg2	analysis					527:534	secretomic analysis	516:534	secretomic analysis	516:534	In this report, secretomic analysis was used to search for the new or interesting enzymes for lignocellulose degradation.
26525014	5	27	theme	lignin	762:767	arg1	degradation					717:727	the degradation	713:727	the degradation of cellulose, hemicellulose, and lignin	713:767	Besides, the utilization ability of P. eryngii to ramie stalk substrate was evaluated from the degradation of cellulose, hemicellulose, and lignin in medium and six extracellular enzymes activities during different growth stages were discussed.
26525014	8	28	theme	lignocellulosic	1339:1353	arg1	hydrolysis					1363:1372	lignocellulosic biomass hydrolysis	1339:1372	lignocellulosic biomass hydrolysis	1339:1372	In addition, we profiled a global view of the secretome of P. eryngii cultivated in ramie stalk media to understand the mechanism behind lignocellulosic biomass hydrolysis.
26525014	1	29	theme	oxidative	173:181	arg1	enzymes					183:189	hydrolytic and oxidative enzymes	158:189	hydrolytic and oxidative enzymes	158:189	Pleurotus eryngii (P. eryngii) can secrete large amount of hydrolytic and oxidative enzymes to degrade lignocellulosic biomass.
26525014	3	30	theme	high-performance	394:409	arg1	enzymes					411:417	high-performance enzymes	394:417	high-performance enzymes	394:417	Identifying more high-performance enzymes or enzyme complexes will lead to efficient in vitro lignocelluloses degradation.
26525014	4	31	theme	new	563:565	arg1	enzymes					582:588	the new or interesting enzymes	559:588	the new or interesting enzymes	559:588	In this report, secretomic analysis was used to search for the new or interesting enzymes for lignocellulose degradation.
26525014	5	32	dep	enzymes	801:807	arg1	discussed					856:864	discussed	856:864	were discussed	851:864	Besides, the utilization ability of P. eryngii to ramie stalk substrate was evaluated from the degradation of cellulose, hemicellulose, and lignin in medium and six extracellular enzymes activities during different growth stages were discussed.
26525014	2	33	from	researches	247:256	arg1	enzymes					287:293	the individual lignolytic enzymes	261:293	the individual lignolytic enzymes	261:293	In spite of several researches on the individual lignolytic enzymes, a direct deconstruction of lignocellulose by enzyme mixture is not yet possible.
26525014	1	34	theme	enzymes	183:189	arg1	enzymes					183:189	hydrolytic and oxidative enzymes	158:189	hydrolytic and oxidative enzymes	158:189	Pleurotus eryngii (P. eryngii) can secrete large amount of hydrolytic and oxidative enzymes to degrade lignocellulosic biomass.
26525014	1	34	theme	enzymes	183:189	arg1	amount					148:153	large amount	142:153	large amount of hydrolytic and oxidative enzymes to degrade lignocellulosic biomass	142:224	Pleurotus eryngii (P. eryngii) can secrete large amount of hydrolytic and oxidative enzymes to degrade lignocellulosic biomass.
26525014	5	35	theme	growth	837:842	arg1	stages					844:849	different growth stages	827:849	different growth stages	827:849	Besides, the utilization ability of P. eryngii to ramie stalk substrate was evaluated from the degradation of cellulose, hemicellulose, and lignin in medium and six extracellular enzymes activities during different growth stages were discussed.
26525014	5	36	theme	different	827:835	arg1	stages					844:849	different growth stages	827:849	different growth stages	827:849	Besides, the utilization ability of P. eryngii to ramie stalk substrate was evaluated from the degradation of cellulose, hemicellulose, and lignin in medium and six extracellular enzymes activities during different growth stages were discussed.
26525014	9	37	theme	diverse	1432:1438	arg1	protease					1518:1525	protease	1518:1525	protease	1518:1525	Eighty-seven nonredundant proteins were identified and a diverse group of enzymes, including cellulases, hemicellulases, pectinase, ligninase, protease, peptidases, and phosphatase implicated in lignocellulose degradation were found.
26525014	9	37	theme	diverse	1432:1438	arg1	group					1440:1444	a diverse group	1430:1444	a diverse group of enzymes, including cellulases, hemicellulases, pectinase, ligninase, protease, peptidases, and phosphatase implicated in lignocellulose degradation	1430:1595	Eighty-seven nonredundant proteins were identified and a diverse group of enzymes, including cellulases, hemicellulases, pectinase, ligninase, protease, peptidases, and phosphatase implicated in lignocellulose degradation were found.
26525014	9	37	theme	diverse	1432:1438	arg1	hemicellulases					1480:1493	hemicellulases	1480:1493	hemicellulases	1480:1493	Eighty-seven nonredundant proteins were identified and a diverse group of enzymes, including cellulases, hemicellulases, pectinase, ligninase, protease, peptidases, and phosphatase implicated in lignocellulose degradation were found.
26525014	9	37	theme	diverse	1432:1438	arg1	enzymes					1449:1455	enzymes	1449:1455	enzymes	1449:1455	Eighty-seven nonredundant proteins were identified and a diverse group of enzymes, including cellulases, hemicellulases, pectinase, ligninase, protease, peptidases, and phosphatase implicated in lignocellulose degradation were found.
26525014	9	37	theme	diverse	1432:1438	arg1	pectinase					1496:1504	pectinase	1496:1504	pectinase	1496:1504	Eighty-seven nonredundant proteins were identified and a diverse group of enzymes, including cellulases, hemicellulases, pectinase, ligninase, protease, peptidases, and phosphatase implicated in lignocellulose degradation were found.
26525014	9	37	theme	diverse	1432:1438	arg1	peptidases					1528:1537	peptidases	1528:1537	peptidases	1528:1537	Eighty-seven nonredundant proteins were identified and a diverse group of enzymes, including cellulases, hemicellulases, pectinase, ligninase, protease, peptidases, and phosphatase implicated in lignocellulose degradation were found.
26525014	9	37	theme	diverse	1432:1438	arg1	phosphatase					1544:1554	phosphatase	1544:1554	phosphatase	1544:1554	Eighty-seven nonredundant proteins were identified and a diverse group of enzymes, including cellulases, hemicellulases, pectinase, ligninase, protease, peptidases, and phosphatase implicated in lignocellulose degradation were found.
26525014	9	37	theme	diverse	1432:1438	arg1	cellulases					1468:1477	cellulases	1468:1477	cellulases	1468:1477	Eighty-seven nonredundant proteins were identified and a diverse group of enzymes, including cellulases, hemicellulases, pectinase, ligninase, protease, peptidases, and phosphatase implicated in lignocellulose degradation were found.
26525014	9	37	theme	diverse	1432:1438	arg1	ligninase					1507:1515	ligninase	1507:1515	ligninase	1507:1515	Eighty-seven nonredundant proteins were identified and a diverse group of enzymes, including cellulases, hemicellulases, pectinase, ligninase, protease, peptidases, and phosphatase implicated in lignocellulose degradation were found.
26525014	2	38	theme	direct	298:303	arg1	deconstruction					305:318	a direct deconstruction	296:318	a direct deconstruction of lignocellulose by enzyme mixture	296:354	In spite of several researches on the individual lignolytic enzymes, a direct deconstruction of lignocellulose by enzyme mixture is not yet possible.
26525014	2	38	theme	direct	298:303	arg1	possible					367:374	possible	367:374	possible	367:374	In spite of several researches on the individual lignolytic enzymes, a direct deconstruction of lignocellulose by enzyme mixture is not yet possible.
26525014	3	39	theme	lignocelluloses	471:485	arg1	degradation					487:497	efficient in vitro lignocelluloses degradation	452:497	efficient in vitro lignocelluloses degradation	452:497	Identifying more high-performance enzymes or enzyme complexes will lead to efficient in vitro lignocelluloses degradation.
26525014	4	40	theme	interesting	570:580	arg1	enzymes					582:588	the new or interesting enzymes	559:588	the new or interesting enzymes	559:588	In this report, secretomic analysis was used to search for the new or interesting enzymes for lignocellulose degradation.
26525014	7	41	theme	Enzyme	1055:1060	arg1	activity					1062:1069	Enzyme activity	1055:1069	Enzyme activity	1055:1069	Enzyme activity showed that carboxymethyl cellulase, xylanase, laccase, and peroxidase activity peaks appeared at the primordial initiation stage.
26525014	8	42	theme	biomass	1355:1361	arg1	hydrolysis					1363:1372	lignocellulosic biomass hydrolysis	1339:1372	lignocellulosic biomass hydrolysis	1339:1372	In addition, we profiled a global view of the secretome of P. eryngii cultivated in ramie stalk media to understand the mechanism behind lignocellulosic biomass hydrolysis.
26525014	7	43	theme	peroxidase	1131:1140	arg1	activity					1142:1149	peroxidase activity	1131:1149	peroxidase activity	1131:1149	Enzyme activity showed that carboxymethyl cellulase, xylanase, laccase, and peroxidase activity peaks appeared at the primordial initiation stage.
26525014	8	44	theme	ramie	1286:1290	arg1	media					1298:1302	ramie stalk media	1286:1302	ramie stalk media	1286:1302	In addition, we profiled a global view of the secretome of P. eryngii cultivated in ramie stalk media to understand the mechanism behind lignocellulosic biomass hydrolysis.
26525014	3	45	theme	enzyme	422:427	arg1	complexes					429:437	enzyme complexes	422:437	enzyme complexes	422:437	Identifying more high-performance enzymes or enzyme complexes will lead to efficient in vitro lignocelluloses degradation.
26525014	10	46	theme	lignocelluloses	1696:1710	arg1	mechanisms					1724:1733	the lignocelluloses degradation mechanisms	1692:1733	the lignocelluloses degradation mechanisms of P. eryngii	1692:1747	In conclusion, the information in this report will be helpful to better understand the lignocelluloses degradation mechanisms of P. eryngii.
26525014	3	47	theme	in	462:463	arg1	degradation					487:497	efficient in vitro lignocelluloses degradation	452:497	efficient in vitro lignocelluloses degradation	452:497	Identifying more high-performance enzymes or enzyme complexes will lead to efficient in vitro lignocelluloses degradation.
26525014	9	48	theme	enzymes	1449:1455	arg1	protease					1518:1525	protease	1518:1525	protease	1518:1525	Eighty-seven nonredundant proteins were identified and a diverse group of enzymes, including cellulases, hemicellulases, pectinase, ligninase, protease, peptidases, and phosphatase implicated in lignocellulose degradation were found.
26525014	9	48	theme	enzymes	1449:1455	arg1	group					1440:1444	a diverse group	1430:1444	a diverse group of enzymes, including cellulases, hemicellulases, pectinase, ligninase, protease, peptidases, and phosphatase implicated in lignocellulose degradation	1430:1595	Eighty-seven nonredundant proteins were identified and a diverse group of enzymes, including cellulases, hemicellulases, pectinase, ligninase, protease, peptidases, and phosphatase implicated in lignocellulose degradation were found.
26525014	9	48	theme	enzymes	1449:1455	arg1	hemicellulases					1480:1493	hemicellulases	1480:1493	hemicellulases	1480:1493	Eighty-seven nonredundant proteins were identified and a diverse group of enzymes, including cellulases, hemicellulases, pectinase, ligninase, protease, peptidases, and phosphatase implicated in lignocellulose degradation were found.
26525014	9	48	theme	enzymes	1449:1455	arg1	enzymes					1449:1455	enzymes	1449:1455	enzymes	1449:1455	Eighty-seven nonredundant proteins were identified and a diverse group of enzymes, including cellulases, hemicellulases, pectinase, ligninase, protease, peptidases, and phosphatase implicated in lignocellulose degradation were found.
26525014	9	48	theme	enzymes	1449:1455	arg1	pectinase					1496:1504	pectinase	1496:1504	pectinase	1496:1504	Eighty-seven nonredundant proteins were identified and a diverse group of enzymes, including cellulases, hemicellulases, pectinase, ligninase, protease, peptidases, and phosphatase implicated in lignocellulose degradation were found.
26525014	9	48	theme	enzymes	1449:1455	arg1	peptidases					1528:1537	peptidases	1528:1537	peptidases	1528:1537	Eighty-seven nonredundant proteins were identified and a diverse group of enzymes, including cellulases, hemicellulases, pectinase, ligninase, protease, peptidases, and phosphatase implicated in lignocellulose degradation were found.
26525014	9	48	theme	enzymes	1449:1455	arg1	phosphatase					1544:1554	phosphatase	1544:1554	phosphatase	1544:1554	Eighty-seven nonredundant proteins were identified and a diverse group of enzymes, including cellulases, hemicellulases, pectinase, ligninase, protease, peptidases, and phosphatase implicated in lignocellulose degradation were found.
26525014	9	48	theme	enzymes	1449:1455	arg1	cellulases					1468:1477	cellulases	1468:1477	cellulases	1468:1477	Eighty-seven nonredundant proteins were identified and a diverse group of enzymes, including cellulases, hemicellulases, pectinase, ligninase, protease, peptidases, and phosphatase implicated in lignocellulose degradation were found.
26525014	9	48	theme	enzymes	1449:1455	arg1	ligninase					1507:1515	ligninase	1507:1515	ligninase	1507:1515	Eighty-seven nonredundant proteins were identified and a diverse group of enzymes, including cellulases, hemicellulases, pectinase, ligninase, protease, peptidases, and phosphatase implicated in lignocellulose degradation were found.
26525014	1	49	theme	lignocellulosic	202:216	arg1	biomass					218:224	lignocellulosic biomass	202:224	lignocellulosic biomass	202:224	Pleurotus eryngii (P. eryngii) can secrete large amount of hydrolytic and oxidative enzymes to degrade lignocellulosic biomass.
26525014	0	50	theme	eryngii	32:38	arg1	analysis					10:17	Secretome analysis	0:17	Secretome analysis of Pleurotus eryngii	0:38	Secretome analysis of Pleurotus eryngii reveals enzymatic composition for ramie stalk degradation.
26525014	6	51	theme	hemicelluloses	952:965	arg1	rates					993:997	cellulose, hemicelluloses, and lignin decomposition rates	941:997	cellulose, hemicelluloses, and lignin decomposition rates of P. eryngii	941:1011	The results showed that a high biological efficiency of 71% was obtained; cellulose, hemicelluloses, and lignin decomposition rates of P. eryngii were 29.2, 26.0, and 51.2%, respectively.
26525014	5	52	theme	cellulose	732:740	arg1	degradation					717:727	the degradation	713:727	the degradation of cellulose, hemicellulose, and lignin	713:767	Besides, the utilization ability of P. eryngii to ramie stalk substrate was evaluated from the degradation of cellulose, hemicellulose, and lignin in medium and six extracellular enzymes activities during different growth stages were discussed.
26525014	8	53	theme	secretome	1248:1256	arg1	view					1236:1239	a global view	1227:1239	a global view of the secretome of P. eryngii cultivated in ramie stalk media to understand the mechanism behind lignocellulosic biomass hydrolysis	1227:1372	In addition, we profiled a global view of the secretome of P. eryngii cultivated in ramie stalk media to understand the mechanism behind lignocellulosic biomass hydrolysis.
26525014	7	54	theme	activity	1142:1149	arg1	peaks					1151:1155	carboxymethyl cellulase, xylanase, laccase, and peroxidase activity peaks	1083:1155	carboxymethyl cellulase, xylanase, laccase, and peroxidase activity peaks	1083:1155	Enzyme activity showed that carboxymethyl cellulase, xylanase, laccase, and peroxidase activity peaks appeared at the primordial initiation stage.
26525014	8	55	theme	eryngii	1264:1270	arg1	secretome					1248:1256	the secretome	1244:1256	the secretome of P. eryngii cultivated in ramie stalk media to understand the mechanism behind lignocellulosic biomass hydrolysis	1244:1372	In addition, we profiled a global view of the secretome of P. eryngii cultivated in ramie stalk media to understand the mechanism behind lignocellulosic biomass hydrolysis.
26525014	7	56	theme	laccase	1118:1124	arg1	peaks					1151:1155	carboxymethyl cellulase, xylanase, laccase, and peroxidase activity peaks	1083:1155	carboxymethyl cellulase, xylanase, laccase, and peroxidase activity peaks	1083:1155	Enzyme activity showed that carboxymethyl cellulase, xylanase, laccase, and peroxidase activity peaks appeared at the primordial initiation stage.
26525014	6	57	theme	lignin	972:977	arg1	decomposition					979:991	lignin decomposition	972:991	lignin decomposition	972:991	The results showed that a high biological efficiency of 71% was obtained; cellulose, hemicelluloses, and lignin decomposition rates of P. eryngii were 29.2, 26.0, and 51.2%, respectively.
26525014	10	58	from	information	1628:1638	arg1	report					1648:1653	this report	1643:1653	this report	1643:1653	In conclusion, the information in this report will be helpful to better understand the lignocelluloses degradation mechanisms of P. eryngii.
26525014	10	59	theme	degradation	1712:1722	arg1	mechanisms					1724:1733	the lignocelluloses degradation mechanisms	1692:1733	the lignocelluloses degradation mechanisms of P. eryngii	1692:1747	In conclusion, the information in this report will be helpful to better understand the lignocelluloses degradation mechanisms of P. eryngii.
26525014	0	60	theme	enzymatic	48:56	arg1	composition					58:68	enzymatic composition	48:68	enzymatic composition for ramie stalk degradation	48:96	Secretome analysis of Pleurotus eryngii reveals enzymatic composition for ramie stalk degradation.
26525014	6	61	theme	cellulose	941:949	arg1	rates					993:997	cellulose, hemicelluloses, and lignin decomposition rates	941:997	cellulose, hemicelluloses, and lignin decomposition rates of P. eryngii	941:1011	The results showed that a high biological efficiency of 71% was obtained; cellulose, hemicelluloses, and lignin decomposition rates of P. eryngii were 29.2, 26.0, and 51.2%, respectively.
26525014	2	62	theme	enzyme	341:346	arg1	mixture					348:354	enzyme mixture	341:354	enzyme mixture	341:354	In spite of several researches on the individual lignolytic enzymes, a direct deconstruction of lignocellulose by enzyme mixture is not yet possible.
26525014	3	63	dep	in	462:463	arg1	vitro					465:469	vitro	465:469	vitro	465:469	Identifying more high-performance enzymes or enzyme complexes will lead to efficient in vitro lignocelluloses degradation.
26525014	9	64	theme	lignocellulose	1570:1583	arg1	degradation					1585:1595	lignocellulose degradation	1570:1595	lignocellulose degradation	1570:1595	Eighty-seven nonredundant proteins were identified and a diverse group of enzymes, including cellulases, hemicellulases, pectinase, ligninase, protease, peptidases, and phosphatase implicated in lignocellulose degradation were found.
26525014	10	65	theme	eryngii	1741:1747	arg1	mechanisms					1724:1733	the lignocelluloses degradation mechanisms	1692:1733	the lignocelluloses degradation mechanisms of P. eryngii	1692:1747	In conclusion, the information in this report will be helpful to better understand the lignocelluloses degradation mechanisms of P. eryngii.
26525014	7	66	theme	cellulase	1097:1105	arg1	peaks					1151:1155	carboxymethyl cellulase, xylanase, laccase, and peroxidase activity peaks	1083:1155	carboxymethyl cellulase, xylanase, laccase, and peroxidase activity peaks	1083:1155	Enzyme activity showed that carboxymethyl cellulase, xylanase, laccase, and peroxidase activity peaks appeared at the primordial initiation stage.
26525014	2	67	theme	lignocellulose	323:336	arg1	deconstruction					305:318	a direct deconstruction	296:318	a direct deconstruction of lignocellulose by enzyme mixture	296:354	In spite of several researches on the individual lignolytic enzymes, a direct deconstruction of lignocellulose by enzyme mixture is not yet possible.
26525014	2	67	theme	lignocellulose	323:336	arg1	possible					367:374	possible	367:374	possible	367:374	In spite of several researches on the individual lignolytic enzymes, a direct deconstruction of lignocellulose by enzyme mixture is not yet possible.
26525014	9	68	theme	Eighty-seven	1375:1386	arg1	proteins					1401:1408	Eighty-seven nonredundant proteins	1375:1408	Eighty-seven nonredundant proteins	1375:1408	Eighty-seven nonredundant proteins were identified and a diverse group of enzymes, including cellulases, hemicellulases, pectinase, ligninase, protease, peptidases, and phosphatase implicated in lignocellulose degradation were found.
26525014	0	69	theme	ramie	74:78	arg1	degradation					86:96	ramie stalk degradation	74:96	ramie stalk degradation	74:96	Secretome analysis of Pleurotus eryngii reveals enzymatic composition for ramie stalk degradation.
26525014	8	70	dep	mechanism	1322:1330	arg1	behind					1332:1337	behind	1332:1337	behind lignocellulosic biomass hydrolysis	1332:1372	In addition, we profiled a global view of the secretome of P. eryngii cultivated in ramie stalk media to understand the mechanism behind lignocellulosic biomass hydrolysis.
26525014	1	71	dep	eryngii	109:115	arg1	eryngii					121:127	P. eryngii	118:127	P. eryngii	118:127	Pleurotus eryngii (P. eryngii) can secrete large amount of hydrolytic and oxidative enzymes to degrade lignocellulosic biomass.
26525014	9	72	theme	nonredundant	1388:1399	arg1	proteins					1401:1408	Eighty-seven nonredundant proteins	1375:1408	Eighty-seven nonredundant proteins	1375:1408	Eighty-seven nonredundant proteins were identified and a diverse group of enzymes, including cellulases, hemicellulases, pectinase, ligninase, protease, peptidases, and phosphatase implicated in lignocellulose degradation were found.
26525014	4	73	theme	secretomic	516:525	arg1	analysis					527:534	secretomic analysis	516:534	secretomic analysis	516:534	In this report, secretomic analysis was used to search for the new or interesting enzymes for lignocellulose degradation.
26525014	2	74	theme	several	239:245	arg1	researches					247:256	several researches	239:256	several researches on the individual lignolytic enzymes	239:293	In spite of several researches on the individual lignolytic enzymes, a direct deconstruction of lignocellulose by enzyme mixture is not yet possible.
26525014	1	75	theme	large	142:146	arg1	enzymes					183:189	hydrolytic and oxidative enzymes	158:189	hydrolytic and oxidative enzymes	158:189	Pleurotus eryngii (P. eryngii) can secrete large amount of hydrolytic and oxidative enzymes to degrade lignocellulosic biomass.
26525014	1	75	theme	large	142:146	arg1	amount					148:153	large amount	142:153	large amount of hydrolytic and oxidative enzymes to degrade lignocellulosic biomass	142:224	Pleurotus eryngii (P. eryngii) can secrete large amount of hydrolytic and oxidative enzymes to degrade lignocellulosic biomass.
28738662	4	0	theme	Fe3O4	566:570	arg1	core					572:575	chitosan layer decorated Fe3O4 core	541:575	chitosan layer decorated Fe3O4 core	541:575	Fe3O4-CS@G composite holds chitosan layer decorated Fe3O4 core, which improves the hydrophilicity of materials greatly.
28738662	7	1	dep	enriched	1043:1050	arg1	compared					1103:1110	compared	1103:1110	compared with previously reported magnetic graphene composites	1103:1164	Using Fe3O4-CS@G, four standard proteins Cyt-c, BSA, Myo and OVA were enriched with better adsorption capacity and recovery rate, compared with previously reported magnetic graphene composites.
28738662	11	2	theme	human	1609:1613	arg1	plasma					1615:1620	human plasma	1609:1620	human plasma	1609:1620	Eventually, 123 proteins were identified from one of SAX fractions of human plasma, which is much better than commercial Sep-pak C18 enrichment column (39 proteins).
28738662	6	3	theme	sufficient	806:815	arg1	opportunities					825:837	the sufficient contact opportunities	802:837	the sufficient contact opportunities between graphene composites and proteins	802:878	The good water dispersibility ensures the sufficient contact opportunities between graphene composites and proteins, and the large surface area provides enough adsorption sites for the enrichment of proteins.
28738662	9	4	theme	universal	1396:1404	arg1	enrichment					1420:1429	the universal and efficient enrichment	1392:1429	the universal and efficient enrichment of proteins	1392:1441	proteins adsorption on Fe3O4-CS@G was further studied, which indicates that hydrophobic and electrostatic interaction work together to facilitate the universal and efficient enrichment of proteins.
28738662	12	5	theme	outstanding	1715:1725	arg1	performances					1727:1738	All these outstanding performances	1705:1738	All these outstanding performances	1705:1738	All these outstanding performances suggest that Fe3O4-CS@G is an ideal platform for the enrichment of low-abundance intact proteins and thus holds great potential to facilitate the identification of biomarkers from biological samples in proteomics research.
28738662	7	6	theme	recovery	1088:1095	arg1	rate					1097:1100	recovery rate	1088:1100	recovery rate	1088:1100	Using Fe3O4-CS@G, four standard proteins Cyt-c, BSA, Myo and OVA were enriched with better adsorption capacity and recovery rate, compared with previously reported magnetic graphene composites.
28738662	1	7	theme	biological	266:275	arg1	samples					277:283	biological samples	266:283	biological samples	266:283	In proteomics research, the screening and monitoring of disease biomarkers is still a major challenge, mainly due to their low concentration in biological samples.
28738662	6	8	theme	large	889:893	arg1	area					903:906	the large surface area	885:906	the large surface area	885:906	The good water dispersibility ensures the sufficient contact opportunities between graphene composites and proteins, and the large surface area provides enough adsorption sites for the enrichment of proteins.
28738662	6	9	theme	water	773:777	arg1	dispersibility					779:792	The good water dispersibility	764:792	The good water dispersibility	764:792	The good water dispersibility ensures the sufficient contact opportunities between graphene composites and proteins, and the large surface area provides enough adsorption sites for the enrichment of proteins.
28738662	1	10	theme	biomarkers	186:195	arg1	challenge					214:222	a major challenge	206:222	a major challenge	206:222	In proteomics research, the screening and monitoring of disease biomarkers is still a major challenge, mainly due to their low concentration in biological samples.
28738662	1	10	theme	biomarkers	186:195	arg1	monitoring					164:173	monitoring	164:173	monitoring	164:173	In proteomics research, the screening and monitoring of disease biomarkers is still a major challenge, mainly due to their low concentration in biological samples.
28738662	1	10	theme	biomarkers	186:195	arg1	screening					150:158	screening	150:158	screening	150:158	In proteomics research, the screening and monitoring of disease biomarkers is still a major challenge, mainly due to their low concentration in biological samples.
28738662	12	11	theme	proteomics	1942:1951	arg1	research					1953:1960	proteomics research	1942:1960	proteomics research	1942:1960	All these outstanding performances suggest that Fe3O4-CS@G is an ideal platform for the enrichment of low-abundance intact proteins and thus holds great potential to facilitate the identification of biomarkers from biological samples in proteomics research.
28738662	10	12	theme	Fe3O4-CS	1527:1534	arg1	G					1536:1536	Fe3O4-CS@G	1527:1536	Fe3O4-CS@G	1527:1536	Human plasma sample was employed to further evaluate the enrichment performance of Fe3O4-CS@G.
28738662	7	13	theme	better	1057:1062	arg1	capacity					1075:1082	better adsorption capacity	1057:1082	better adsorption capacity	1057:1082	Using Fe3O4-CS@G, four standard proteins Cyt-c, BSA, Myo and OVA were enriched with better adsorption capacity and recovery rate, compared with previously reported magnetic graphene composites.
28738662	4	14	theme	materials	615:623	arg1	hydrophilicity					597:610	the hydrophilicity	593:610	the hydrophilicity of materials	593:623	Fe3O4-CS@G composite holds chitosan layer decorated Fe3O4 core, which improves the hydrophilicity of materials greatly.
28738662	1	15	theme	major	208:212	arg1	monitoring					164:173	monitoring	164:173	monitoring	164:173	In proteomics research, the screening and monitoring of disease biomarkers is still a major challenge, mainly due to their low concentration in biological samples.
28738662	1	15	theme	major	208:212	arg1	challenge					214:222	a major challenge	206:222	a major challenge	206:222	In proteomics research, the screening and monitoring of disease biomarkers is still a major challenge, mainly due to their low concentration in biological samples.
28738662	1	15	theme	major	208:212	arg1	screening					150:158	screening	150:158	screening	150:158	In proteomics research, the screening and monitoring of disease biomarkers is still a major challenge, mainly due to their low concentration in biological samples.
28738662	1	16	from	concentration	249:261	arg1	samples					277:283	biological samples	266:283	biological samples	266:283	In proteomics research, the screening and monitoring of disease biomarkers is still a major challenge, mainly due to their low concentration in biological samples.
28738662	1	17	from	challenge	214:222	arg1	research					136:143	proteomics research	125:143	proteomics research	125:143	In proteomics research, the screening and monitoring of disease biomarkers is still a major challenge, mainly due to their low concentration in biological samples.
28738662	9	18	from	adsorption	1255:1264	arg1	G					1278:1278	Fe3O4-CS@G	1269:1278	Fe3O4-CS@G	1269:1278	proteins adsorption on Fe3O4-CS@G was further studied, which indicates that hydrophobic and electrostatic interaction work together to facilitate the universal and efficient enrichment of proteins.
28738662	12	19	from	identification	1886:1899	arg1	samples					1931:1937	biological samples	1920:1937	biological samples in proteomics research	1920:1960	All these outstanding performances suggest that Fe3O4-CS@G is an ideal platform for the enrichment of low-abundance intact proteins and thus holds great potential to facilitate the identification of biomarkers from biological samples in proteomics research.
28738662	0	20	theme	core-shell	64:73	arg1	composites					110:119	core-shell structured Fe3O4-chitosan@graphene composites	64:119	core-shell structured Fe3O4-chitosan@graphene composites	64:119	Highly efficient enrichment of low-abundance intact proteins by core-shell structured Fe3O4-chitosan@graphene composites.
28738662	7	21	theme	Fe3O4-CS	979:986	arg1	G					988:988	Fe3O4-CS@G	979:988	Fe3O4-CS@G	979:988	Using Fe3O4-CS@G, four standard proteins Cyt-c, BSA, Myo and OVA were enriched with better adsorption capacity and recovery rate, compared with previously reported magnetic graphene composites.
28738662	0	22	theme	Fe3O4-chitosan	86:99	arg1	composites					110:119	core-shell structured Fe3O4-chitosan@graphene composites	64:119	core-shell structured Fe3O4-chitosan@graphene composites	64:119	Highly efficient enrichment of low-abundance intact proteins by core-shell structured Fe3O4-chitosan@graphene composites.
28738662	9	23	theme	Fe3O4-CS	1269:1276	arg1	G					1278:1278	Fe3O4-CS@G	1269:1278	Fe3O4-CS@G	1269:1278	proteins adsorption on Fe3O4-CS@G was further studied, which indicates that hydrophobic and electrostatic interaction work together to facilitate the universal and efficient enrichment of proteins.
28738662	5	24	theme	graphene	649:656	arg1	shell					669:673	the graphene nanosheets shell	645:673	the graphene nanosheets shell formed via electrostatic assembly	645:707	Meanwhile, the graphene nanosheets shell formed via electrostatic assembly endows the composite with huge surface area (178m2/g).
28738662	5	24	theme	graphene	649:656	arg1	Meanwhile					634:642	Meanwhile	634:642	Meanwhile	634:642	Meanwhile, the graphene nanosheets shell formed via electrostatic assembly endows the composite with huge surface area (178m2/g).
28738662	0	25	theme	graphene	101:108	arg1	composites					110:119	core-shell structured Fe3O4-chitosan@graphene composites	64:119	core-shell structured Fe3O4-chitosan@graphene composites	64:119	Highly efficient enrichment of low-abundance intact proteins by core-shell structured Fe3O4-chitosan@graphene composites.
28738662	9	26	theme	efficient	1410:1418	arg1	enrichment					1420:1429	the universal and efficient enrichment	1392:1429	the universal and efficient enrichment of proteins	1392:1441	proteins adsorption on Fe3O4-CS@G was further studied, which indicates that hydrophobic and electrostatic interaction work together to facilitate the universal and efficient enrichment of proteins.
28738662	10	27	theme	plasma	1450:1455	arg1	sample					1457:1462	Human plasma sample	1444:1462	Human plasma sample	1444:1462	Human plasma sample was employed to further evaluate the enrichment performance of Fe3O4-CS@G.
28738662	3	28	theme	@	412:412	arg1	G					432:432	Fe3O4-CS@G	423:432	Fe3O4-CS@G	423:432	In this work, we developed a Fe3O4-chitosan@graphene (Fe3O4-CS@G) core-shell composite to enrich low-abundance proteins from biological samples.
28738662	3	28	theme	@	412:412	arg1	composite					446:454	a Fe3O4-chitosan@graphene (Fe3O4-CS@G) core-shell composite	396:454	a Fe3O4-chitosan@graphene (Fe3O4-CS@G) core-shell composite to enrich low-abundance proteins from biological samples	396:511	In this work, we developed a Fe3O4-chitosan@graphene (Fe3O4-CS@G) core-shell composite to enrich low-abundance proteins from biological samples.
28738662	1	29	dep	screening	150:158	arg1	the					146:148	the	146:148	the	146:148	In proteomics research, the screening and monitoring of disease biomarkers is still a major challenge, mainly due to their low concentration in biological samples.
28738662	7	30	theme	graphene	1146:1153	arg1	composites					1155:1164	previously reported magnetic graphene composites	1117:1164	previously reported magnetic graphene composites	1117:1164	Using Fe3O4-CS@G, four standard proteins Cyt-c, BSA, Myo and OVA were enriched with better adsorption capacity and recovery rate, compared with previously reported magnetic graphene composites.
28738662	12	31	theme	intact	1821:1826	arg1	proteins					1828:1835	low-abundance intact proteins	1807:1835	low-abundance intact proteins	1807:1835	All these outstanding performances suggest that Fe3O4-CS@G is an ideal platform for the enrichment of low-abundance intact proteins and thus holds great potential to facilitate the identification of biomarkers from biological samples in proteomics research.
28738662	4	32	theme	chitosan	541:548	arg1	core					572:575	chitosan layer decorated Fe3O4 core	541:575	chitosan layer decorated Fe3O4 core	541:575	Fe3O4-CS@G composite holds chitosan layer decorated Fe3O4 core, which improves the hydrophilicity of materials greatly.
28738662	11	33	theme	enrichment	1672:1681	arg1	column					1683:1688	commercial Sep-pak C18 enrichment column	1649:1688	commercial Sep-pak C18 enrichment column (39 proteins)	1649:1702	Eventually, 123 proteins were identified from one of SAX fractions of human plasma, which is much better than commercial Sep-pak C18 enrichment column (39 proteins).
28738662	11	33	theme	enrichment	1672:1681	arg1	proteins					1694:1701	39 proteins	1691:1701	39 proteins	1691:1701	Eventually, 123 proteins were identified from one of SAX fractions of human plasma, which is much better than commercial Sep-pak C18 enrichment column (39 proteins).
28738662	3	34	theme	@	431:431	arg1	G					432:432	Fe3O4-CS@G	423:432	Fe3O4-CS@G	423:432	In this work, we developed a Fe3O4-chitosan@graphene (Fe3O4-CS@G) core-shell composite to enrich low-abundance proteins from biological samples.
28738662	3	34	theme	@	431:431	arg1	composite					446:454	a Fe3O4-chitosan@graphene (Fe3O4-CS@G) core-shell composite	396:454	a Fe3O4-chitosan@graphene (Fe3O4-CS@G) core-shell composite to enrich low-abundance proteins from biological samples	396:511	In this work, we developed a Fe3O4-chitosan@graphene (Fe3O4-CS@G) core-shell composite to enrich low-abundance proteins from biological samples.
28738662	7	35	theme	reported	1128:1135	arg1	composites					1155:1164	previously reported magnetic graphene composites	1117:1164	previously reported magnetic graphene composites	1117:1164	Using Fe3O4-CS@G, four standard proteins Cyt-c, BSA, Myo and OVA were enriched with better adsorption capacity and recovery rate, compared with previously reported magnetic graphene composites.
28738662	9	36	theme	hydrophobic	1322:1332	arg1	interaction					1352:1362	hydrophobic and electrostatic interaction	1322:1362	hydrophobic and electrostatic interaction	1322:1362	proteins adsorption on Fe3O4-CS@G was further studied, which indicates that hydrophobic and electrostatic interaction work together to facilitate the universal and efficient enrichment of proteins.
28738662	0	37	theme	low-abundance	31:43	arg1	proteins					52:59	low-abundance intact proteins	31:59	low-abundance intact proteins	31:59	Highly efficient enrichment of low-abundance intact proteins by core-shell structured Fe3O4-chitosan@graphene composites.
28738662	12	38	theme	ideal	1770:1774	arg1	G					1762:1762	Fe3O4-CS@G	1753:1762	Fe3O4-CS@G	1753:1762	All these outstanding performances suggest that Fe3O4-CS@G is an ideal platform for the enrichment of low-abundance intact proteins and thus holds great potential to facilitate the identification of biomarkers from biological samples in proteomics research.
28738662	12	38	theme	ideal	1770:1774	arg1	platform					1776:1783	an ideal platform	1767:1783	an ideal platform for the enrichment of low-abundance intact proteins	1767:1835	All these outstanding performances suggest that Fe3O4-CS@G is an ideal platform for the enrichment of low-abundance intact proteins and thus holds great potential to facilitate the identification of biomarkers from biological samples in proteomics research.
28738662	11	39	theme	Sep-pak	1660:1666	arg1	column					1683:1688	commercial Sep-pak C18 enrichment column	1649:1688	commercial Sep-pak C18 enrichment column (39 proteins)	1649:1702	Eventually, 123 proteins were identified from one of SAX fractions of human plasma, which is much better than commercial Sep-pak C18 enrichment column (39 proteins).
28738662	11	39	theme	Sep-pak	1660:1666	arg1	proteins					1694:1701	39 proteins	1691:1701	39 proteins	1691:1701	Eventually, 123 proteins were identified from one of SAX fractions of human plasma, which is much better than commercial Sep-pak C18 enrichment column (39 proteins).
28738662	0	40	theme	proteins	52:59	arg1	enrichment					17:26	Highly efficient enrichment	0:26	Highly efficient enrichment of low-abundance intact proteins by core-shell structured Fe3O4-chitosan@graphene composites.	0:120	Highly efficient enrichment of low-abundance intact proteins by core-shell structured Fe3O4-chitosan@graphene composites.
28738662	9	41	theme	electrostatic	1338:1350	arg1	interaction					1352:1362	hydrophobic and electrostatic interaction	1322:1362	hydrophobic and electrostatic interaction	1322:1362	proteins adsorption on Fe3O4-CS@G was further studied, which indicates that hydrophobic and electrostatic interaction work together to facilitate the universal and efficient enrichment of proteins.
28738662	6	42	theme	graphene	847:854	arg1	composites					856:865	graphene composites	847:865	graphene composites	847:865	The good water dispersibility ensures the sufficient contact opportunities between graphene composites and proteins, and the large surface area provides enough adsorption sites for the enrichment of proteins.
28738662	4	43	theme	@	522:522	arg1	composite					525:533	Fe3O4-CS@G composite	514:533	Fe3O4-CS@G composite	514:533	Fe3O4-CS@G composite holds chitosan layer decorated Fe3O4 core, which improves the hydrophilicity of materials greatly.
28738662	10	44	theme	enrichment	1501:1510	arg1	performance					1512:1522	the enrichment performance	1497:1522	the enrichment performance of Fe3O4-CS@G	1497:1536	Human plasma sample was employed to further evaluate the enrichment performance of Fe3O4-CS@G.
28738662	2	45	theme	proteins	330:337	arg1	enrichment					309:318	the universal enrichment	295:318	the universal enrichment of intact proteins	295:337	However, the universal enrichment of intact proteins has not been further studied.
28738662	12	46	theme	@	1761:1761	arg1	G					1762:1762	Fe3O4-CS@G	1753:1762	Fe3O4-CS@G	1753:1762	All these outstanding performances suggest that Fe3O4-CS@G is an ideal platform for the enrichment of low-abundance intact proteins and thus holds great potential to facilitate the identification of biomarkers from biological samples in proteomics research.
28738662	12	46	theme	@	1761:1761	arg1	platform					1776:1783	an ideal platform	1767:1783	an ideal platform for the enrichment of low-abundance intact proteins	1767:1835	All these outstanding performances suggest that Fe3O4-CS@G is an ideal platform for the enrichment of low-abundance intact proteins and thus holds great potential to facilitate the identification of biomarkers from biological samples in proteomics research.
28738662	3	47	theme	biological	494:503	arg1	samples					505:511	biological samples	494:511	biological samples	494:511	In this work, we developed a Fe3O4-chitosan@graphene (Fe3O4-CS@G) core-shell composite to enrich low-abundance proteins from biological samples.
28738662	6	48	theme	adsorption	924:933	arg1	sites					935:939	adsorption sites	924:939	adsorption sites for the enrichment of proteins	924:970	The good water dispersibility ensures the sufficient contact opportunities between graphene composites and proteins, and the large surface area provides enough adsorption sites for the enrichment of proteins.
28738662	10	49	theme	@	1535:1535	arg1	G					1536:1536	Fe3O4-CS@G	1527:1536	Fe3O4-CS@G	1527:1536	Human plasma sample was employed to further evaluate the enrichment performance of Fe3O4-CS@G.
28738662	11	50	theme	SAX	1592:1594	arg1	fractions					1596:1604	SAX fractions	1592:1604	SAX fractions of human plasma, which is much better than commercial Sep-pak C18 enrichment column (39 proteins)	1592:1702	Eventually, 123 proteins were identified from one of SAX fractions of human plasma, which is much better than commercial Sep-pak C18 enrichment column (39 proteins).
28738662	1	51	theme	low	245:247	arg1	concentration					249:261	their low concentration	239:261	their low concentration in biological samples	239:283	In proteomics research, the screening and monitoring of disease biomarkers is still a major challenge, mainly due to their low concentration in biological samples.
28738662	4	52	theme	decorated	556:564	arg1	core					572:575	chitosan layer decorated Fe3O4 core	541:575	chitosan layer decorated Fe3O4 core	541:575	Fe3O4-CS@G composite holds chitosan layer decorated Fe3O4 core, which improves the hydrophilicity of materials greatly.
28738662	11	53	theme	plasma	1615:1620	arg1	fractions					1596:1604	SAX fractions	1592:1604	SAX fractions of human plasma, which is much better than commercial Sep-pak C18 enrichment column (39 proteins)	1592:1702	Eventually, 123 proteins were identified from one of SAX fractions of human plasma, which is much better than commercial Sep-pak C18 enrichment column (39 proteins).
28738662	6	54	theme	surface	895:901	arg1	area					903:906	the large surface area	885:906	the large surface area	885:906	The good water dispersibility ensures the sufficient contact opportunities between graphene composites and proteins, and the large surface area provides enough adsorption sites for the enrichment of proteins.
28738662	7	55	theme	adsorption	1064:1073	arg1	capacity					1075:1082	better adsorption capacity	1057:1082	better adsorption capacity	1057:1082	Using Fe3O4-CS@G, four standard proteins Cyt-c, BSA, Myo and OVA were enriched with better adsorption capacity and recovery rate, compared with previously reported magnetic graphene composites.
28738662	3	56	from	samples	505:511	arg1	proteins					480:487	low-abundance proteins	466:487	low-abundance proteins from biological samples	466:511	In this work, we developed a Fe3O4-chitosan@graphene (Fe3O4-CS@G) core-shell composite to enrich low-abundance proteins from biological samples.
28738662	1	57	theme	disease	178:184	arg1	biomarkers					186:195	disease biomarkers	178:195	disease biomarkers	178:195	In proteomics research, the screening and monitoring of disease biomarkers is still a major challenge, mainly due to their low concentration in biological samples.
28738662	6	58	theme	good	768:771	arg1	dispersibility					779:792	The good water dispersibility	764:792	The good water dispersibility	764:792	The good water dispersibility ensures the sufficient contact opportunities between graphene composites and proteins, and the large surface area provides enough adsorption sites for the enrichment of proteins.
28738662	9	59	theme	proteins	1434:1441	arg1	enrichment					1420:1429	the universal and efficient enrichment	1392:1429	the universal and efficient enrichment of proteins	1392:1441	proteins adsorption on Fe3O4-CS@G was further studied, which indicates that hydrophobic and electrostatic interaction work together to facilitate the universal and efficient enrichment of proteins.
28738662	12	60	from	samples	1931:1937	arg1	research					1953:1960	proteomics research	1942:1960	proteomics research	1942:1960	All these outstanding performances suggest that Fe3O4-CS@G is an ideal platform for the enrichment of low-abundance intact proteins and thus holds great potential to facilitate the identification of biomarkers from biological samples in proteomics research.
28738662	12	60	from	samples	1931:1937	arg1	biomarkers					1904:1913	biomarkers	1904:1913	biomarkers from biological samples in proteomics research	1904:1960	All these outstanding performances suggest that Fe3O4-CS@G is an ideal platform for the enrichment of low-abundance intact proteins and thus holds great potential to facilitate the identification of biomarkers from biological samples in proteomics research.
28738662	12	60	from	samples	1931:1937	arg1	identification					1886:1899	the identification	1882:1899	the identification of biomarkers from biological samples in proteomics research	1882:1960	All these outstanding performances suggest that Fe3O4-CS@G is an ideal platform for the enrichment of low-abundance intact proteins and thus holds great potential to facilitate the identification of biomarkers from biological samples in proteomics research.
28738662	5	61	theme	electrostatic	686:698	arg1	assembly					700:707	electrostatic assembly	686:707	electrostatic assembly	686:707	Meanwhile, the graphene nanosheets shell formed via electrostatic assembly endows the composite with huge surface area (178m2/g).
28738662	2	62	theme	universal	299:307	arg1	enrichment					309:318	the universal enrichment	295:318	the universal enrichment of intact proteins	295:337	However, the universal enrichment of intact proteins has not been further studied.
28738662	6	63	theme	proteins	963:970	arg1	enrichment					949:958	the enrichment	945:958	the enrichment of proteins	945:970	The good water dispersibility ensures the sufficient contact opportunities between graphene composites and proteins, and the large surface area provides enough adsorption sites for the enrichment of proteins.
28738662	12	64	theme	biomarkers	1904:1913	arg1	identification					1886:1899	the identification	1882:1899	the identification of biomarkers from biological samples in proteomics research	1882:1960	All these outstanding performances suggest that Fe3O4-CS@G is an ideal platform for the enrichment of low-abundance intact proteins and thus holds great potential to facilitate the identification of biomarkers from biological samples in proteomics research.
28738662	5	65	theme	nanosheets	658:667	arg1	shell					669:673	the graphene nanosheets shell	645:673	the graphene nanosheets shell formed via electrostatic assembly	645:707	Meanwhile, the graphene nanosheets shell formed via electrostatic assembly endows the composite with huge surface area (178m2/g).
28738662	5	65	theme	nanosheets	658:667	arg1	Meanwhile					634:642	Meanwhile	634:642	Meanwhile	634:642	Meanwhile, the graphene nanosheets shell formed via electrostatic assembly endows the composite with huge surface area (178m2/g).
28738662	1	66	theme	proteomics	125:134	arg1	research					136:143	proteomics research	125:143	proteomics research	125:143	In proteomics research, the screening and monitoring of disease biomarkers is still a major challenge, mainly due to their low concentration in biological samples.
28738662	12	67	theme	biological	1920:1929	arg1	samples					1931:1937	biological samples	1920:1937	biological samples in proteomics research	1920:1960	All these outstanding performances suggest that Fe3O4-CS@G is an ideal platform for the enrichment of low-abundance intact proteins and thus holds great potential to facilitate the identification of biomarkers from biological samples in proteomics research.
28738662	7	68	theme	standard	996:1003	arg1	OVA					1034:1036	OVA	1034:1036	OVA	1034:1036	Using Fe3O4-CS@G, four standard proteins Cyt-c, BSA, Myo and OVA were enriched with better adsorption capacity and recovery rate, compared with previously reported magnetic graphene composites.
28738662	7	68	theme	standard	996:1003	arg1	Myo					1026:1028	Myo	1026:1028	Myo	1026:1028	Using Fe3O4-CS@G, four standard proteins Cyt-c, BSA, Myo and OVA were enriched with better adsorption capacity and recovery rate, compared with previously reported magnetic graphene composites.
28738662	7	68	theme	standard	996:1003	arg1	proteins					1005:1012	four standard proteins	991:1012	four standard proteins Cyt-c, BSA, Myo and OVA	991:1036	Using Fe3O4-CS@G, four standard proteins Cyt-c, BSA, Myo and OVA were enriched with better adsorption capacity and recovery rate, compared with previously reported magnetic graphene composites.
28738662	7	68	theme	standard	996:1003	arg1	BSA					1021:1023	BSA	1021:1023	BSA	1021:1023	Using Fe3O4-CS@G, four standard proteins Cyt-c, BSA, Myo and OVA were enriched with better adsorption capacity and recovery rate, compared with previously reported magnetic graphene composites.
28738662	7	68	theme	standard	996:1003	arg1	Cyt-c					1014:1018	Cyt-c	1014:1018	Cyt-c	1014:1018	Using Fe3O4-CS@G, four standard proteins Cyt-c, BSA, Myo and OVA were enriched with better adsorption capacity and recovery rate, compared with previously reported magnetic graphene composites.
28738662	0	69	theme	structured	75:84	arg1	composites					110:119	core-shell structured Fe3O4-chitosan@graphene composites	64:119	core-shell structured Fe3O4-chitosan@graphene composites	64:119	Highly efficient enrichment of low-abundance intact proteins by core-shell structured Fe3O4-chitosan@graphene composites.
28738662	9	70	theme	proteins	1246:1253	arg1	adsorption					1255:1264	proteins adsorption	1246:1264	proteins adsorption on Fe3O4-CS@G	1246:1278	proteins adsorption on Fe3O4-CS@G was further studied, which indicates that hydrophobic and electrostatic interaction work together to facilitate the universal and efficient enrichment of proteins.
28738662	0	71	theme	@	100:100	arg1	composites					110:119	core-shell structured Fe3O4-chitosan@graphene composites	64:119	core-shell structured Fe3O4-chitosan@graphene composites	64:119	Highly efficient enrichment of low-abundance intact proteins by core-shell structured Fe3O4-chitosan@graphene composites.
28738662	7	72	theme	@	987:987	arg1	G					988:988	Fe3O4-CS@G	979:988	Fe3O4-CS@G	979:988	Using Fe3O4-CS@G, four standard proteins Cyt-c, BSA, Myo and OVA were enriched with better adsorption capacity and recovery rate, compared with previously reported magnetic graphene composites.
28738662	9	73	theme	@	1277:1277	arg1	G					1278:1278	Fe3O4-CS@G	1269:1278	Fe3O4-CS@G	1269:1278	proteins adsorption on Fe3O4-CS@G was further studied, which indicates that hydrophobic and electrostatic interaction work together to facilitate the universal and efficient enrichment of proteins.
28738662	5	74	theme	huge	735:738	arg1	178m2/g					754:760	178m2/g	754:760	178m2/g	754:760	Meanwhile, the graphene nanosheets shell formed via electrostatic assembly endows the composite with huge surface area (178m2/g).
28738662	5	74	theme	huge	735:738	arg1	area					748:751	huge surface area	735:751	huge surface area (178m2/g)	735:761	Meanwhile, the graphene nanosheets shell formed via electrostatic assembly endows the composite with huge surface area (178m2/g).
28738662	12	75	theme	great	1852:1856	arg1	potential					1858:1866	great potential	1852:1866	great potential to facilitate the identification of biomarkers from biological samples in proteomics research	1852:1960	All these outstanding performances suggest that Fe3O4-CS@G is an ideal platform for the enrichment of low-abundance intact proteins and thus holds great potential to facilitate the identification of biomarkers from biological samples in proteomics research.
28738662	7	76	dep	proteins	1005:1012	arg1	OVA					1034:1036	OVA	1034:1036	OVA	1034:1036	Using Fe3O4-CS@G, four standard proteins Cyt-c, BSA, Myo and OVA were enriched with better adsorption capacity and recovery rate, compared with previously reported magnetic graphene composites.
28738662	7	76	dep	proteins	1005:1012	arg1	Myo					1026:1028	Myo	1026:1028	Myo	1026:1028	Using Fe3O4-CS@G, four standard proteins Cyt-c, BSA, Myo and OVA were enriched with better adsorption capacity and recovery rate, compared with previously reported magnetic graphene composites.
28738662	7	76	dep	proteins	1005:1012	arg1	proteins					1005:1012	four standard proteins	991:1012	four standard proteins Cyt-c, BSA, Myo and OVA	991:1036	Using Fe3O4-CS@G, four standard proteins Cyt-c, BSA, Myo and OVA were enriched with better adsorption capacity and recovery rate, compared with previously reported magnetic graphene composites.
28738662	7	76	dep	proteins	1005:1012	arg1	BSA					1021:1023	BSA	1021:1023	BSA	1021:1023	Using Fe3O4-CS@G, four standard proteins Cyt-c, BSA, Myo and OVA were enriched with better adsorption capacity and recovery rate, compared with previously reported magnetic graphene composites.
28738662	7	76	dep	proteins	1005:1012	arg1	Cyt-c					1014:1018	Cyt-c	1014:1018	Cyt-c	1014:1018	Using Fe3O4-CS@G, four standard proteins Cyt-c, BSA, Myo and OVA were enriched with better adsorption capacity and recovery rate, compared with previously reported magnetic graphene composites.
28738662	3	77	theme	Fe3O4-CS	423:430	arg1	G					432:432	Fe3O4-CS@G	423:432	Fe3O4-CS@G	423:432	In this work, we developed a Fe3O4-chitosan@graphene (Fe3O4-CS@G) core-shell composite to enrich low-abundance proteins from biological samples.
28738662	3	77	theme	Fe3O4-CS	423:430	arg1	composite					446:454	a Fe3O4-chitosan@graphene (Fe3O4-CS@G) core-shell composite	396:454	a Fe3O4-chitosan@graphene (Fe3O4-CS@G) core-shell composite to enrich low-abundance proteins from biological samples	396:511	In this work, we developed a Fe3O4-chitosan@graphene (Fe3O4-CS@G) core-shell composite to enrich low-abundance proteins from biological samples.
28738662	0	78	theme	efficient	7:15	arg1	enrichment					17:26	Highly efficient enrichment	0:26	Highly efficient enrichment of low-abundance intact proteins by core-shell structured Fe3O4-chitosan@graphene composites.	0:120	Highly efficient enrichment of low-abundance intact proteins by core-shell structured Fe3O4-chitosan@graphene composites.
28738662	10	79	theme	Human	1444:1448	arg1	sample					1457:1462	Human plasma sample	1444:1462	Human plasma sample	1444:1462	Human plasma sample was employed to further evaluate the enrichment performance of Fe3O4-CS@G.
28738662	3	80	theme	graphene	413:420	arg1	G					432:432	Fe3O4-CS@G	423:432	Fe3O4-CS@G	423:432	In this work, we developed a Fe3O4-chitosan@graphene (Fe3O4-CS@G) core-shell composite to enrich low-abundance proteins from biological samples.
28738662	3	80	theme	graphene	413:420	arg1	composite					446:454	a Fe3O4-chitosan@graphene (Fe3O4-CS@G) core-shell composite	396:454	a Fe3O4-chitosan@graphene (Fe3O4-CS@G) core-shell composite to enrich low-abundance proteins from biological samples	396:511	In this work, we developed a Fe3O4-chitosan@graphene (Fe3O4-CS@G) core-shell composite to enrich low-abundance proteins from biological samples.
28738662	7	81	theme	magnetic	1137:1144	arg1	composites					1155:1164	previously reported magnetic graphene composites	1117:1164	previously reported magnetic graphene composites	1117:1164	Using Fe3O4-CS@G, four standard proteins Cyt-c, BSA, Myo and OVA were enriched with better adsorption capacity and recovery rate, compared with previously reported magnetic graphene composites.
28738662	12	82	theme	proteins	1828:1835	arg1	enrichment					1793:1802	the enrichment	1789:1802	the enrichment of low-abundance intact proteins	1789:1835	All these outstanding performances suggest that Fe3O4-CS@G is an ideal platform for the enrichment of low-abundance intact proteins and thus holds great potential to facilitate the identification of biomarkers from biological samples in proteomics research.
28738662	4	83	theme	layer	550:554	arg1	core					572:575	chitosan layer decorated Fe3O4 core	541:575	chitosan layer decorated Fe3O4 core	541:575	Fe3O4-CS@G composite holds chitosan layer decorated Fe3O4 core, which improves the hydrophilicity of materials greatly.
28738662	11	84	theme	C18	1668:1670	arg1	column					1683:1688	commercial Sep-pak C18 enrichment column	1649:1688	commercial Sep-pak C18 enrichment column (39 proteins)	1649:1702	Eventually, 123 proteins were identified from one of SAX fractions of human plasma, which is much better than commercial Sep-pak C18 enrichment column (39 proteins).
28738662	11	84	theme	C18	1668:1670	arg1	proteins					1694:1701	39 proteins	1691:1701	39 proteins	1691:1701	Eventually, 123 proteins were identified from one of SAX fractions of human plasma, which is much better than commercial Sep-pak C18 enrichment column (39 proteins).
28738662	3	85	theme	core-shell	435:444	arg1	G					432:432	Fe3O4-CS@G	423:432	Fe3O4-CS@G	423:432	In this work, we developed a Fe3O4-chitosan@graphene (Fe3O4-CS@G) core-shell composite to enrich low-abundance proteins from biological samples.
28738662	3	85	theme	core-shell	435:444	arg1	composite					446:454	a Fe3O4-chitosan@graphene (Fe3O4-CS@G) core-shell composite	396:454	a Fe3O4-chitosan@graphene (Fe3O4-CS@G) core-shell composite to enrich low-abundance proteins from biological samples	396:511	In this work, we developed a Fe3O4-chitosan@graphene (Fe3O4-CS@G) core-shell composite to enrich low-abundance proteins from biological samples.
28738662	0	86	theme	intact	45:50	arg1	proteins					52:59	low-abundance intact proteins	31:59	low-abundance intact proteins	31:59	Highly efficient enrichment of low-abundance intact proteins by core-shell structured Fe3O4-chitosan@graphene composites.
28738662	3	87	theme	Fe3O4-chitosan	398:411	arg1	G					432:432	Fe3O4-CS@G	423:432	Fe3O4-CS@G	423:432	In this work, we developed a Fe3O4-chitosan@graphene (Fe3O4-CS@G) core-shell composite to enrich low-abundance proteins from biological samples.
28738662	3	87	theme	Fe3O4-chitosan	398:411	arg1	composite					446:454	a Fe3O4-chitosan@graphene (Fe3O4-CS@G) core-shell composite	396:454	a Fe3O4-chitosan@graphene (Fe3O4-CS@G) core-shell composite to enrich low-abundance proteins from biological samples	396:511	In this work, we developed a Fe3O4-chitosan@graphene (Fe3O4-CS@G) core-shell composite to enrich low-abundance proteins from biological samples.
28738662	4	88	theme	Fe3O4-CS	514:521	arg1	composite					525:533	Fe3O4-CS@G composite	514:533	Fe3O4-CS@G composite	514:533	Fe3O4-CS@G composite holds chitosan layer decorated Fe3O4 core, which improves the hydrophilicity of materials greatly.
28738662	11	89	theme	commercial	1649:1658	arg1	column					1683:1688	commercial Sep-pak C18 enrichment column	1649:1688	commercial Sep-pak C18 enrichment column (39 proteins)	1649:1702	Eventually, 123 proteins were identified from one of SAX fractions of human plasma, which is much better than commercial Sep-pak C18 enrichment column (39 proteins).
28738662	11	89	theme	commercial	1649:1658	arg1	proteins					1694:1701	39 proteins	1691:1701	39 proteins	1691:1701	Eventually, 123 proteins were identified from one of SAX fractions of human plasma, which is much better than commercial Sep-pak C18 enrichment column (39 proteins).
28738662	5	90	theme	surface	740:746	arg1	178m2/g					754:760	178m2/g	754:760	178m2/g	754:760	Meanwhile, the graphene nanosheets shell formed via electrostatic assembly endows the composite with huge surface area (178m2/g).
28738662	5	90	theme	surface	740:746	arg1	area					748:751	huge surface area	735:751	huge surface area (178m2/g)	735:761	Meanwhile, the graphene nanosheets shell formed via electrostatic assembly endows the composite with huge surface area (178m2/g).
28738662	4	91	theme	G	523:523	arg1	composite					525:533	Fe3O4-CS@G composite	514:533	Fe3O4-CS@G composite	514:533	Fe3O4-CS@G composite holds chitosan layer decorated Fe3O4 core, which improves the hydrophilicity of materials greatly.
28738662	3	92	theme	low-abundance	466:478	arg1	proteins					480:487	low-abundance proteins	466:487	low-abundance proteins from biological samples	466:511	In this work, we developed a Fe3O4-chitosan@graphene (Fe3O4-CS@G) core-shell composite to enrich low-abundance proteins from biological samples.
28738662	12	93	theme	low-abundance	1807:1819	arg1	proteins					1828:1835	low-abundance intact proteins	1807:1835	low-abundance intact proteins	1807:1835	All these outstanding performances suggest that Fe3O4-CS@G is an ideal platform for the enrichment of low-abundance intact proteins and thus holds great potential to facilitate the identification of biomarkers from biological samples in proteomics research.
28738662	11	94	theme	fractions	1596:1604	arg1	one					1585:1587	one	1585:1587	one	1585:1587	Eventually, 123 proteins were identified from one of SAX fractions of human plasma, which is much better than commercial Sep-pak C18 enrichment column (39 proteins).
28738662	11	94	theme	fractions	1596:1604	arg1	fractions					1596:1604	SAX fractions	1592:1604	SAX fractions of human plasma, which is much better than commercial Sep-pak C18 enrichment column (39 proteins)	1592:1702	Eventually, 123 proteins were identified from one of SAX fractions of human plasma, which is much better than commercial Sep-pak C18 enrichment column (39 proteins).
28738662	2	95	theme	intact	323:328	arg1	proteins					330:337	intact proteins	323:337	intact proteins	323:337	However, the universal enrichment of intact proteins has not been further studied.
28738662	6	96	theme	contact	817:823	arg1	opportunities					825:837	the sufficient contact opportunities	802:837	the sufficient contact opportunities between graphene composites and proteins	802:878	The good water dispersibility ensures the sufficient contact opportunities between graphene composites and proteins, and the large surface area provides enough adsorption sites for the enrichment of proteins.
28738662	12	97	theme	Fe3O4-CS	1753:1760	arg1	G					1762:1762	Fe3O4-CS@G	1753:1762	Fe3O4-CS@G	1753:1762	All these outstanding performances suggest that Fe3O4-CS@G is an ideal platform for the enrichment of low-abundance intact proteins and thus holds great potential to facilitate the identification of biomarkers from biological samples in proteomics research.
28738662	12	97	theme	Fe3O4-CS	1753:1760	arg1	platform					1776:1783	an ideal platform	1767:1783	an ideal platform for the enrichment of low-abundance intact proteins	1767:1835	All these outstanding performances suggest that Fe3O4-CS@G is an ideal platform for the enrichment of low-abundance intact proteins and thus holds great potential to facilitate the identification of biomarkers from biological samples in proteomics research.
28738662	10	98	theme	G	1536:1536	arg1	performance					1512:1522	the enrichment performance	1497:1522	the enrichment performance of Fe3O4-CS@G	1497:1536	Human plasma sample was employed to further evaluate the enrichment performance of Fe3O4-CS@G.
27936719	3	0	theme	wt	295:296	arg1	CNC					300:302	2 wt % CNC	293:302	2 wt % CNC	293:302	Mixtures of 2 wt % CNC + 0.5 wt % MC gave the lowest density foams.
27936719	5	1	theme	draining	563:570	arg1	water					572:576	the draining water	559:576	the draining water	559:576	More than 94% of the added CNC particles remained in the foam phase, not leaving with the draining water.
27936719	6	2	from	coils	645:649	arg1	solution					659:666	solution	659:666	solution	659:666	We propose that the nanoscale CNC particles bind to the larger MC coils both in solution and with MC at the air/water interface, forming weak gels that stabilize air bubbles.
27936719	3	3	theme	%	298:298	arg1	CNC					300:302	2 wt % CNC	293:302	2 wt % CNC	293:302	Mixtures of 2 wt % CNC + 0.5 wt % MC gave the lowest density foams.
27936719	4	4	theme	methylcellulose	456:470	arg1	concentration					439:451	concentration	439:451	concentration of methylcellulose	439:470	This composition sits near the onset of nematic phase formation and also near the overlap concentration of methylcellulose.
27936719	7	5	theme	polyethylene	991:1002	arg1	glycol					1004:1009	polyethylene glycol diacrylate	991:1020	polyethylene glycol diacrylate (PEGDA)	991:1028	Wet CNC-MC foams were sufficiently robust to withstand high temperature (70 °C for 6 h) polymerization of water-soluble monomers giving macroporous CNC composite hydrogels based on acrylamide (AM), 2-hydroxyethyl methacrylate (HEMA), or polyethylene glycol diacrylate (PEGDA).
27936719	7	5	theme	polyethylene	991:1002	arg1	PEGDA					1023:1027	PEGDA	1023:1027	PEGDA	1023:1027	Wet CNC-MC foams were sufficiently robust to withstand high temperature (70 °C for 6 h) polymerization of water-soluble monomers giving macroporous CNC composite hydrogels based on acrylamide (AM), 2-hydroxyethyl methacrylate (HEMA), or polyethylene glycol diacrylate (PEGDA).
27936719	1	6	theme	methylcellulose	151:165	arg1	properties					137:146	the properties	133:146	the properties of methylcellulose (MC) stabilized aqueous foams	133:195	The addition of cellulose nanocrystals (CNC) greatly enhanced the properties of methylcellulose (MC) stabilized aqueous foams.
27936719	7	7	theme	CNC-MC	758:763	arg1	foams					765:769	Wet CNC-MC foams	754:769	Wet CNC-MC foams	754:769	Wet CNC-MC foams were sufficiently robust to withstand high temperature (70 °C for 6 h) polymerization of water-soluble monomers giving macroporous CNC composite hydrogels based on acrylamide (AM), 2-hydroxyethyl methacrylate (HEMA), or polyethylene glycol diacrylate (PEGDA).
27936719	9	8	theme	MC	1246:1247	arg1	forms					1229:1233	commercially available forms	1206:1233	commercially available forms of CNC and MC, already approved for many applications	1206:1287	Finally, our CNC-MC foams are based on commercially available forms of CNC and MC, already approved for many applications.
27936719	3	9	theme	%	313:313	arg1	MC					315:316	0.5 wt % MC	306:316	0.5 wt % MC	306:316	Mixtures of 2 wt % CNC + 0.5 wt % MC gave the lowest density foams.
27936719	8	10	attach	present	1064:1070	arg1	temperatures					1039:1050	high temperatures	1034:1050	high temperatures	1034:1050	At high temperatures, the MC was present as a fibrillar gel phase reinforced by CNC particles, explaining the very high foam stability.
27936719	8	10	attach	present	1064:1070	arg2	MC					1057:1058	the MC	1053:1058	the MC	1053:1058	At high temperatures, the MC was present as a fibrillar gel phase reinforced by CNC particles, explaining the very high foam stability.
27936719	8	10	attach	present	1064:1070	arg2	present					1064:1070	present	1064:1070	present	1064:1070	At high temperatures, the MC was present as a fibrillar gel phase reinforced by CNC particles, explaining the very high foam stability.
27936719	5	11	theme	foam	530:533	arg1	phase					535:539	the foam phase	526:539	the foam phase	526:539	More than 94% of the added CNC particles remained in the foam phase, not leaving with the draining water.
27936719	3	12	theme	MC	315:316	arg1	Mixtures					281:288	Mixtures	281:288	Mixtures of 2 wt % CNC + 0.5 wt % MC	281:316	Mixtures of 2 wt % CNC + 0.5 wt % MC gave the lowest density foams.
27936719	2	13	theme	drainage	265:272	arg1	rates					274:278	drainage rates	265:278	drainage rates	265:278	CNC addition decreased air bubble size, initial foam densities and drainage rates.
27936719	4	14	theme	phase	397:401	arg1	formation					403:411	nematic phase formation	389:411	nematic phase formation	389:411	This composition sits near the onset of nematic phase formation and also near the overlap concentration of methylcellulose.
27936719	8	15	theme	CNC	1111:1113	arg1	particles					1115:1123	CNC particles	1111:1123	CNC particles	1111:1123	At high temperatures, the MC was present as a fibrillar gel phase reinforced by CNC particles, explaining the very high foam stability.
27936719	6	16	with	coils	645:649	arg1	MC					677:678	MC	677:678	MC	677:678	We propose that the nanoscale CNC particles bind to the larger MC coils both in solution and with MC at the air/water interface, forming weak gels that stabilize air bubbles.
27936719	8	17	theme	high	1034:1037	arg1	temperatures					1039:1050	high temperatures	1034:1050	high temperatures	1034:1050	At high temperatures, the MC was present as a fibrillar gel phase reinforced by CNC particles, explaining the very high foam stability.
27936719	6	18	theme	air/water	687:695	arg1	interface					697:705	the air/water interface	683:705	the air/water interface	683:705	We propose that the nanoscale CNC particles bind to the larger MC coils both in solution and with MC at the air/water interface, forming weak gels that stabilize air bubbles.
27936719	7	19	theme	Wet	754:756	arg1	foams					765:769	Wet CNC-MC foams	754:769	Wet CNC-MC foams	754:769	Wet CNC-MC foams were sufficiently robust to withstand high temperature (70 °C for 6 h) polymerization of water-soluble monomers giving macroporous CNC composite hydrogels based on acrylamide (AM), 2-hydroxyethyl methacrylate (HEMA), or polyethylene glycol diacrylate (PEGDA).
27936719	8	20	theme	gel	1087:1089	arg1	phase					1091:1095	a fibrillar gel phase	1075:1095	a fibrillar gel phase reinforced by CNC particles	1075:1123	At high temperatures, the MC was present as a fibrillar gel phase reinforced by CNC particles, explaining the very high foam stability.
27936719	3	21	theme	wt	310:311	arg1	MC					315:316	0.5 wt % MC	306:316	0.5 wt % MC	306:316	Mixtures of 2 wt % CNC + 0.5 wt % MC gave the lowest density foams.
27936719	0	22	from	Nanocrystals	36:47	arg1	Foams					15:19	Stable Aqueous Foams	0:19	Stable Aqueous Foams from Cellulose Nanocrystals and Methyl Cellulose	0:68	Stable Aqueous Foams from Cellulose Nanocrystals and Methyl Cellulose.
27936719	5	23	theme	CNC	500:502	arg1	particles					504:512	the added CNC particles	490:512	the added CNC particles	490:512	More than 94% of the added CNC particles remained in the foam phase, not leaving with the draining water.
27936719	0	24	theme	Stable	0:5	arg1	Foams					15:19	Stable Aqueous Foams	0:19	Stable Aqueous Foams from Cellulose Nanocrystals and Methyl Cellulose	0:68	Stable Aqueous Foams from Cellulose Nanocrystals and Methyl Cellulose.
27936719	8	25	theme	fibrillar	1077:1085	arg1	phase					1091:1095	a fibrillar gel phase	1075:1095	a fibrillar gel phase reinforced by CNC particles	1075:1123	At high temperatures, the MC was present as a fibrillar gel phase reinforced by CNC particles, explaining the very high foam stability.
27936719	0	26	theme	Aqueous	7:13	arg1	Foams					15:19	Stable Aqueous Foams	0:19	Stable Aqueous Foams from Cellulose Nanocrystals and Methyl Cellulose	0:68	Stable Aqueous Foams from Cellulose Nanocrystals and Methyl Cellulose.
27936719	8	27	located	present	1064:1070	arg1	temperatures					1039:1050	high temperatures	1034:1050	high temperatures	1034:1050	At high temperatures, the MC was present as a fibrillar gel phase reinforced by CNC particles, explaining the very high foam stability.
27936719	8	27	located	present	1064:1070	arg2	MC					1057:1058	the MC	1053:1058	the MC	1053:1058	At high temperatures, the MC was present as a fibrillar gel phase reinforced by CNC particles, explaining the very high foam stability.
27936719	8	27	located	present	1064:1070	arg2	present					1064:1070	present	1064:1070	present	1064:1070	At high temperatures, the MC was present as a fibrillar gel phase reinforced by CNC particles, explaining the very high foam stability.
27936719	7	28	theme	2-hydroxyethyl	952:965	arg1	HEMA					981:984	HEMA	981:984	HEMA	981:984	Wet CNC-MC foams were sufficiently robust to withstand high temperature (70 °C for 6 h) polymerization of water-soluble monomers giving macroporous CNC composite hydrogels based on acrylamide (AM), 2-hydroxyethyl methacrylate (HEMA), or polyethylene glycol diacrylate (PEGDA).
27936719	7	28	theme	2-hydroxyethyl	952:965	arg1	methacrylate					967:978	2-hydroxyethyl methacrylate	952:978	2-hydroxyethyl methacrylate (HEMA)	952:985	Wet CNC-MC foams were sufficiently robust to withstand high temperature (70 °C for 6 h) polymerization of water-soluble monomers giving macroporous CNC composite hydrogels based on acrylamide (AM), 2-hydroxyethyl methacrylate (HEMA), or polyethylene glycol diacrylate (PEGDA).
27936719	5	29	theme	particles	504:512	arg1	particles					504:512	the added CNC particles	490:512	the added CNC particles	490:512	More than 94% of the added CNC particles remained in the foam phase, not leaving with the draining water.
27936719	5	29	theme	particles	504:512	arg1	%					485:485	More than 94%	473:485	More than 94% of the added CNC particles	473:512	More than 94% of the added CNC particles remained in the foam phase, not leaving with the draining water.
27936719	6	30	theme	CNC	609:611	arg1	particles					613:621	the nanoscale CNC particles	595:621	the nanoscale CNC particles	595:621	We propose that the nanoscale CNC particles bind to the larger MC coils both in solution and with MC at the air/water interface, forming weak gels that stabilize air bubbles.
27936719	9	31	theme	many	1271:1274	arg1	applications					1276:1287	many applications	1271:1287	many applications	1271:1287	Finally, our CNC-MC foams are based on commercially available forms of CNC and MC, already approved for many applications.
27936719	3	32	theme	density	334:340	arg1	foams					342:346	the lowest density foams	323:346	the lowest density foams	323:346	Mixtures of 2 wt % CNC + 0.5 wt % MC gave the lowest density foams.
27936719	4	33	dep	the	427:429	arg1	overlap					431:437	overlap	431:437	overlap concentration of methylcellulose	431:470	This composition sits near the onset of nematic phase formation and also near the overlap concentration of methylcellulose.
27936719	7	34	theme	temperature	814:824	arg1	polymerization					842:855	withstand high temperature (70 °C for 6 h) polymerization	799:855	withstand high temperature (70 °C for 6 h) polymerization of water-soluble monomers giving macroporous CNC composite hydrogels based on acrylamide (AM), 2-hydroxyethyl methacrylate (HEMA), or polyethylene glycol diacrylate (PEGDA)	799:1028	Wet CNC-MC foams were sufficiently robust to withstand high temperature (70 °C for 6 h) polymerization of water-soluble monomers giving macroporous CNC composite hydrogels based on acrylamide (AM), 2-hydroxyethyl methacrylate (HEMA), or polyethylene glycol diacrylate (PEGDA).
27936719	1	35	theme	cellulose	87:95	arg1	CNC					111:113	CNC	111:113	CNC	111:113	The addition of cellulose nanocrystals (CNC) greatly enhanced the properties of methylcellulose (MC) stabilized aqueous foams.
27936719	1	35	theme	cellulose	87:95	arg1	nanocrystals					97:108	cellulose nanocrystals	87:108	cellulose nanocrystals (CNC)	87:114	The addition of cellulose nanocrystals (CNC) greatly enhanced the properties of methylcellulose (MC) stabilized aqueous foams.
27936719	6	36	theme	nanoscale	599:607	arg1	particles					613:621	the nanoscale CNC particles	595:621	the nanoscale CNC particles	595:621	We propose that the nanoscale CNC particles bind to the larger MC coils both in solution and with MC at the air/water interface, forming weak gels that stabilize air bubbles.
27936719	9	37	theme	approved	1258:1265	arg1	CNC					1238:1240	CNC	1238:1240	CNC	1238:1240	Finally, our CNC-MC foams are based on commercially available forms of CNC and MC, already approved for many applications.
27936719	1	38	theme	nanocrystals	97:108	arg1	addition					75:82	The addition	71:82	The addition of cellulose nanocrystals (CNC)	71:114	The addition of cellulose nanocrystals (CNC) greatly enhanced the properties of methylcellulose (MC) stabilized aqueous foams.
27936719	1	39	theme	aqueous	183:189	arg1	foams					191:195	aqueous foams	183:195	aqueous foams	183:195	The addition of cellulose nanocrystals (CNC) greatly enhanced the properties of methylcellulose (MC) stabilized aqueous foams.
27936719	7	40	theme	monomers	874:881	arg1	polymerization					842:855	withstand high temperature (70 °C for 6 h) polymerization	799:855	withstand high temperature (70 °C for 6 h) polymerization of water-soluble monomers giving macroporous CNC composite hydrogels based on acrylamide (AM), 2-hydroxyethyl methacrylate (HEMA), or polyethylene glycol diacrylate (PEGDA)	799:1028	Wet CNC-MC foams were sufficiently robust to withstand high temperature (70 °C for 6 h) polymerization of water-soluble monomers giving macroporous CNC composite hydrogels based on acrylamide (AM), 2-hydroxyethyl methacrylate (HEMA), or polyethylene glycol diacrylate (PEGDA).
27936719	9	41	theme	CNC-MC	1180:1185	arg1	foams					1187:1191	our CNC-MC foams	1176:1191	our CNC-MC foams	1176:1191	Finally, our CNC-MC foams are based on commercially available forms of CNC and MC, already approved for many applications.
27936719	10	42	theme	"	1313:1313	arg1	This					1290:1293	This	1290:1293	This	1290:1293	This is a "shovel-ready" technology.
27936719	10	42	theme	"	1313:1313	arg1	technology					1315:1324	a "shovel-ready" technology	1298:1324	a "shovel-ready" technology	1298:1324	This is a "shovel-ready" technology.
27936719	4	43	theme	nematic	389:395	arg1	formation					403:411	nematic phase formation	389:411	nematic phase formation	389:411	This composition sits near the onset of nematic phase formation and also near the overlap concentration of methylcellulose.
27936719	10	44	theme	shovel-ready	1301:1312	arg1	This					1290:1293	This	1290:1293	This	1290:1293	This is a "shovel-ready" technology.
27936719	10	44	theme	shovel-ready	1301:1312	arg1	technology					1315:1324	a "shovel-ready" technology	1298:1324	a "shovel-ready" technology	1298:1324	This is a "shovel-ready" technology.
27936719	2	45	theme	bubble	225:230	arg1	size					232:235	air bubble size	221:235	air bubble size	221:235	CNC addition decreased air bubble size, initial foam densities and drainage rates.
27936719	0	46	theme	Cellulose	26:34	arg1	Nanocrystals					36:47	Cellulose Nanocrystals	26:47	Cellulose Nanocrystals	26:47	Stable Aqueous Foams from Cellulose Nanocrystals and Methyl Cellulose.
27936719	4	47	theme	formation	403:411	arg1	onset					380:384	the onset	376:384	the onset of nematic phase formation	376:411	This composition sits near the onset of nematic phase formation and also near the overlap concentration of methylcellulose.
27936719	2	48	theme	air	221:223	arg1	size					232:235	air bubble size	221:235	air bubble size	221:235	CNC addition decreased air bubble size, initial foam densities and drainage rates.
27936719	7	49	theme	CNC	902:904	arg1	hydrogels					916:924	macroporous CNC composite hydrogels	890:924	macroporous CNC composite hydrogels based on acrylamide (AM), 2-hydroxyethyl methacrylate (HEMA), or polyethylene glycol diacrylate (PEGDA)	890:1028	Wet CNC-MC foams were sufficiently robust to withstand high temperature (70 °C for 6 h) polymerization of water-soluble monomers giving macroporous CNC composite hydrogels based on acrylamide (AM), 2-hydroxyethyl methacrylate (HEMA), or polyethylene glycol diacrylate (PEGDA).
27936719	0	50	theme	Methyl	53:58	arg1	Cellulose					60:68	Methyl Cellulose	53:68	Methyl Cellulose	53:68	Stable Aqueous Foams from Cellulose Nanocrystals and Methyl Cellulose.
27936719	0	51	from	Cellulose	60:68	arg1	Foams					15:19	Stable Aqueous Foams	0:19	Stable Aqueous Foams from Cellulose Nanocrystals and Methyl Cellulose	0:68	Stable Aqueous Foams from Cellulose Nanocrystals and Methyl Cellulose.
27936719	7	52	theme	composite	906:914	arg1	hydrogels					916:924	macroporous CNC composite hydrogels	890:924	macroporous CNC composite hydrogels based on acrylamide (AM), 2-hydroxyethyl methacrylate (HEMA), or polyethylene glycol diacrylate (PEGDA)	890:1028	Wet CNC-MC foams were sufficiently robust to withstand high temperature (70 °C for 6 h) polymerization of water-soluble monomers giving macroporous CNC composite hydrogels based on acrylamide (AM), 2-hydroxyethyl methacrylate (HEMA), or polyethylene glycol diacrylate (PEGDA).
27936719	7	53	theme	diacrylate	1011:1020	arg1	glycol					1004:1009	polyethylene glycol diacrylate	991:1020	polyethylene glycol diacrylate (PEGDA)	991:1028	Wet CNC-MC foams were sufficiently robust to withstand high temperature (70 °C for 6 h) polymerization of water-soluble monomers giving macroporous CNC composite hydrogels based on acrylamide (AM), 2-hydroxyethyl methacrylate (HEMA), or polyethylene glycol diacrylate (PEGDA).
27936719	7	53	theme	diacrylate	1011:1020	arg1	PEGDA					1023:1027	PEGDA	1023:1027	PEGDA	1023:1027	Wet CNC-MC foams were sufficiently robust to withstand high temperature (70 °C for 6 h) polymerization of water-soluble monomers giving macroporous CNC composite hydrogels based on acrylamide (AM), 2-hydroxyethyl methacrylate (HEMA), or polyethylene glycol diacrylate (PEGDA).
27936719	2	54	theme	CNC	198:200	arg1	addition					202:209	CNC addition	198:209	CNC addition	198:209	CNC addition decreased air bubble size, initial foam densities and drainage rates.
27936719	7	55	theme	withstand	799:807	arg1	polymerization					842:855	withstand high temperature (70 °C for 6 h) polymerization	799:855	withstand high temperature (70 °C for 6 h) polymerization of water-soluble monomers giving macroporous CNC composite hydrogels based on acrylamide (AM), 2-hydroxyethyl methacrylate (HEMA), or polyethylene glycol diacrylate (PEGDA)	799:1028	Wet CNC-MC foams were sufficiently robust to withstand high temperature (70 °C for 6 h) polymerization of water-soluble monomers giving macroporous CNC composite hydrogels based on acrylamide (AM), 2-hydroxyethyl methacrylate (HEMA), or polyethylene glycol diacrylate (PEGDA).
27936719	2	56	theme	foam	246:249	arg1	densities					251:259	initial foam densities	238:259	initial foam densities	238:259	CNC addition decreased air bubble size, initial foam densities and drainage rates.
27936719	5	57	theme	added	494:498	arg1	particles					504:512	the added CNC particles	490:512	the added CNC particles	490:512	More than 94% of the added CNC particles remained in the foam phase, not leaving with the draining water.
27936719	8	58	theme	high	1146:1149	arg1	stability					1156:1164	the very high foam stability	1137:1164	the very high foam stability	1137:1164	At high temperatures, the MC was present as a fibrillar gel phase reinforced by CNC particles, explaining the very high foam stability.
27936719	7	59	theme	high	809:812	arg1	temperature					814:824	high temperature	809:824	withstand high temperature (70 °C for 6 h) polymerization of water-soluble monomers giving macroporous CNC composite hydrogels based on acrylamide (AM), 2-hydroxyethyl methacrylate (HEMA), or polyethylene glycol diacrylate (PEGDA)	799:1028	Wet CNC-MC foams were sufficiently robust to withstand high temperature (70 °C for 6 h) polymerization of water-soluble monomers giving macroporous CNC composite hydrogels based on acrylamide (AM), 2-hydroxyethyl methacrylate (HEMA), or polyethylene glycol diacrylate (PEGDA).
27936719	9	60	theme	available	1219:1227	arg1	forms					1229:1233	commercially available forms	1206:1233	commercially available forms of CNC and MC, already approved for many applications	1206:1287	Finally, our CNC-MC foams are based on commercially available forms of CNC and MC, already approved for many applications.
27936719	7	61	theme	macroporous	890:900	arg1	hydrogels					916:924	macroporous CNC composite hydrogels	890:924	macroporous CNC composite hydrogels based on acrylamide (AM), 2-hydroxyethyl methacrylate (HEMA), or polyethylene glycol diacrylate (PEGDA)	890:1028	Wet CNC-MC foams were sufficiently robust to withstand high temperature (70 °C for 6 h) polymerization of water-soluble monomers giving macroporous CNC composite hydrogels based on acrylamide (AM), 2-hydroxyethyl methacrylate (HEMA), or polyethylene glycol diacrylate (PEGDA).
27936719	2	62	theme	initial	238:244	arg1	densities					251:259	initial foam densities	238:259	initial foam densities	238:259	CNC addition decreased air bubble size, initial foam densities and drainage rates.
27936719	7	63	theme	water-soluble	860:872	arg1	monomers					874:881	water-soluble monomers	860:881	water-soluble monomers giving macroporous CNC composite hydrogels based on acrylamide (AM), 2-hydroxyethyl methacrylate (HEMA), or polyethylene glycol diacrylate (PEGDA)	860:1028	Wet CNC-MC foams were sufficiently robust to withstand high temperature (70 °C for 6 h) polymerization of water-soluble monomers giving macroporous CNC composite hydrogels based on acrylamide (AM), 2-hydroxyethyl methacrylate (HEMA), or polyethylene glycol diacrylate (PEGDA).
27936719	8	64	theme	foam	1151:1154	arg1	stability					1156:1164	the very high foam stability	1137:1164	the very high foam stability	1137:1164	At high temperatures, the MC was present as a fibrillar gel phase reinforced by CNC particles, explaining the very high foam stability.
27936719	3	65	theme	CNC	300:302	arg1	Mixtures					281:288	Mixtures	281:288	Mixtures of 2 wt % CNC + 0.5 wt % MC	281:316	Mixtures of 2 wt % CNC + 0.5 wt % MC gave the lowest density foams.
27936719	6	66	theme	weak	716:719	arg1	gels					721:724	weak gels	716:724	weak gels that stabilize air bubbles	716:751	We propose that the nanoscale CNC particles bind to the larger MC coils both in solution and with MC at the air/water interface, forming weak gels that stabilize air bubbles.
27936719	6	67	theme	MC	642:643	arg1	coils					645:649	the larger MC coils	631:649	the larger MC coils both in solution and with MC	631:678	We propose that the nanoscale CNC particles bind to the larger MC coils both in solution and with MC at the air/water interface, forming weak gels that stabilize air bubbles.
27936719	6	68	theme	air	741:743	arg1	bubbles					745:751	air bubbles	741:751	air bubbles	741:751	We propose that the nanoscale CNC particles bind to the larger MC coils both in solution and with MC at the air/water interface, forming weak gels that stabilize air bubbles.
27936719	8	69	from	temperatures	1039:1050	arg1	present					1064:1070	present	1064:1070	present	1064:1070	At high temperatures, the MC was present as a fibrillar gel phase reinforced by CNC particles, explaining the very high foam stability.
27936719	8	69	from	temperatures	1039:1050	arg1	MC					1057:1058	the MC	1053:1058	the MC	1053:1058	At high temperatures, the MC was present as a fibrillar gel phase reinforced by CNC particles, explaining the very high foam stability.
27936719	6	70	theme	larger	635:640	arg1	coils					645:649	the larger MC coils	631:649	the larger MC coils both in solution and with MC	631:678	We propose that the nanoscale CNC particles bind to the larger MC coils both in solution and with MC at the air/water interface, forming weak gels that stabilize air bubbles.
27936719	3	71	theme	lowest	327:332	arg1	foams					342:346	the lowest density foams	323:346	the lowest density foams	323:346	Mixtures of 2 wt % CNC + 0.5 wt % MC gave the lowest density foams.
27936719	7	72	dep	temperature	814:824	arg1	°C					830:831	70 °C	827:831	70 °C	827:831	Wet CNC-MC foams were sufficiently robust to withstand high temperature (70 °C for 6 h) polymerization of water-soluble monomers giving macroporous CNC composite hydrogels based on acrylamide (AM), 2-hydroxyethyl methacrylate (HEMA), or polyethylene glycol diacrylate (PEGDA).
27936719	9	73	theme	CNC	1238:1240	arg1	forms					1229:1233	commercially available forms	1206:1233	commercially available forms of CNC and MC, already approved for many applications	1206:1287	Finally, our CNC-MC foams are based on commercially available forms of CNC and MC, already approved for many applications.
28522008	1	0	theme	bio-renewable	113:125	arg1	sources					127:133	[Hmim][HSO4] ionic liquid (IL) and bio-renewable sources	78:133	[Hmim][HSO4] ionic liquid (IL) and bio-renewable sources as chitosan (CHT) and chondroitin sulfate (CS)	78:180	[Hmim][HSO4] ionic liquid (IL) and bio-renewable sources as chitosan (CHT) and chondroitin sulfate (CS) were used to yield hydrogel-based materials (CHT/CS).
28522008	2	1	theme	systems	396:402	arg1	production					367:376	the production	363:376	the production of CHT/CS blended systems	363:402	The use of IL to solubilize both polysaccharides was considered an innovative way based on "green chemistry" principle, aiming the production of CHT/CS blended systems.
28522008	4	2	from	capacities	605:614	arg1	range					581:585	the 1.2-10pH range	568:585	the 1.2-10pH range	568:585	The hydrogels were characterized and achieved with excellent stabilities (in the 1.2-10pH range), larger swelling capacities, as well as devoid of cytotoxicity towards the normal VERO and diseased HT29 cells.
28522008	5	3	theme	technological	774:786	arg1	purposes					788:795	many technological purposes	769:795	many technological purposes	769:795	The CHT/CS hydrogels carried out in [Hmim][HSO4] could be applied in many technological purposes, like medical, pharmaceutical, and environmental fields.
28522008	3	4	theme	short	466:470	arg1	times					484:488	short dissolution times	466:488	short dissolution times	466:488	CHT/CS hydrogels were carried out in homogeneous medium from short dissolution times.
28522008	1	5	theme	hydrogel-based	201:214	arg1	CHT/CS					227:232	CHT/CS	227:232	CHT/CS	227:232	[Hmim][HSO4] ionic liquid (IL) and bio-renewable sources as chitosan (CHT) and chondroitin sulfate (CS) were used to yield hydrogel-based materials (CHT/CS).
28522008	1	5	theme	hydrogel-based	201:214	arg1	materials					216:224	hydrogel-based materials	201:224	hydrogel-based materials (CHT/CS)	201:233	[Hmim][HSO4] ionic liquid (IL) and bio-renewable sources as chitosan (CHT) and chondroitin sulfate (CS) were used to yield hydrogel-based materials (CHT/CS).
28522008	3	6	theme	CHT/CS	405:410	arg1	hydrogels					412:420	CHT/CS hydrogels	405:420	CHT/CS hydrogels	405:420	CHT/CS hydrogels were carried out in homogeneous medium from short dissolution times.
28522008	1	7	dep	ionic	91:95	arg1	IL					105:106	IL	105:106	IL	105:106	[Hmim][HSO4] ionic liquid (IL) and bio-renewable sources as chitosan (CHT) and chondroitin sulfate (CS) were used to yield hydrogel-based materials (CHT/CS).
28522008	1	7	dep	ionic	91:95	arg1	liquid					97:102	liquid	97:102	liquid	97:102	[Hmim][HSO4] ionic liquid (IL) and bio-renewable sources as chitosan (CHT) and chondroitin sulfate (CS) were used to yield hydrogel-based materials (CHT/CS).
28522008	4	8	theme	larger	589:594	arg1	capacities					605:614	larger swelling capacities	589:614	larger swelling capacities	589:614	The hydrogels were characterized and achieved with excellent stabilities (in the 1.2-10pH range), larger swelling capacities, as well as devoid of cytotoxicity towards the normal VERO and diseased HT29 cells.
28522008	4	9	theme	diseased	679:686	arg1	cells					693:697	the normal VERO and diseased HT29 cells	659:697	cells	693:697	The hydrogels were characterized and achieved with excellent stabilities (in the 1.2-10pH range), larger swelling capacities, as well as devoid of cytotoxicity towards the normal VERO and diseased HT29 cells.
28522008	0	10	theme	sulfate	21:27	arg1	hydrogels					29:37	Chitosan/chondroitin sulfate hydrogels	0:37	Chitosan/chondroitin sulfate hydrogels prepared in [Hmim][HSO4]	0:62	Chitosan/chondroitin sulfate hydrogels prepared in [Hmim][HSO4] ionic liquid.
28522008	5	11	theme	environmental	832:844	arg1	fields					846:851	medical, pharmaceutical, and environmental fields	803:851	medical, pharmaceutical, and environmental fields	803:851	The CHT/CS hydrogels carried out in [Hmim][HSO4] could be applied in many technological purposes, like medical, pharmaceutical, and environmental fields.
28522008	4	12	theme	1.2-10pH	572:579	arg1	range					581:585	the 1.2-10pH range	568:585	the 1.2-10pH range	568:585	The hydrogels were characterized and achieved with excellent stabilities (in the 1.2-10pH range), larger swelling capacities, as well as devoid of cytotoxicity towards the normal VERO and diseased HT29 cells.
28522008	0	13	theme	Chitosan/chondroitin	0:19	arg1	hydrogels					29:37	Chitosan/chondroitin sulfate hydrogels	0:37	Chitosan/chondroitin sulfate hydrogels prepared in [Hmim][HSO4]	0:62	Chitosan/chondroitin sulfate hydrogels prepared in [Hmim][HSO4] ionic liquid.
28522008	4	14	theme	devoid	628:633	arg1	stabilities					552:562	excellent stabilities	542:562	excellent stabilities (in the 1.2-10pH range)	542:586	The hydrogels were characterized and achieved with excellent stabilities (in the 1.2-10pH range), larger swelling capacities, as well as devoid of cytotoxicity towards the normal VERO and diseased HT29 cells.
28522008	4	15	theme	cytotoxicity	638:649	arg1	devoid					628:633	devoid	628:633	devoid	628:633	The hydrogels were characterized and achieved with excellent stabilities (in the 1.2-10pH range), larger swelling capacities, as well as devoid of cytotoxicity towards the normal VERO and diseased HT29 cells.
28522008	2	16	theme	innovative	303:312	arg1	way					314:316	an innovative way	300:316	an innovative way based on "green chemistry" principle, aiming the production of CHT/CS blended systems	300:402	The use of IL to solubilize both polysaccharides was considered an innovative way based on "green chemistry" principle, aiming the production of CHT/CS blended systems.
28522008	5	17	theme	pharmaceutical	812:825	arg1	fields					846:851	medical, pharmaceutical, and environmental fields	803:851	medical, pharmaceutical, and environmental fields	803:851	The CHT/CS hydrogels carried out in [Hmim][HSO4] could be applied in many technological purposes, like medical, pharmaceutical, and environmental fields.
28522008	3	18	theme	dissolution	472:482	arg1	times					484:488	short dissolution times	466:488	short dissolution times	466:488	CHT/CS hydrogels were carried out in homogeneous medium from short dissolution times.
28522008	2	19	theme	"	343:343	arg1	principle					345:353	"green chemistry" principle	327:353	"green chemistry" principle	327:353	The use of IL to solubilize both polysaccharides was considered an innovative way based on "green chemistry" principle, aiming the production of CHT/CS blended systems.
28522008	3	20	theme	homogeneous	442:452	arg1	medium					454:459	homogeneous medium	442:459	homogeneous medium from short dissolution times	442:488	CHT/CS hydrogels were carried out in homogeneous medium from short dissolution times.
28522008	2	21	theme	chemistry	334:342	arg1	principle					345:353	"green chemistry" principle	327:353	"green chemistry" principle	327:353	The use of IL to solubilize both polysaccharides was considered an innovative way based on "green chemistry" principle, aiming the production of CHT/CS blended systems.
28522008	2	22	theme	blended	388:394	arg1	systems					396:402	CHT/CS blended systems	381:402	CHT/CS blended systems	381:402	The use of IL to solubilize both polysaccharides was considered an innovative way based on "green chemistry" principle, aiming the production of CHT/CS blended systems.
28522008	1	23	theme	chondroitin	157:167	arg1	CS					178:179	CS	178:179	CS	178:179	[Hmim][HSO4] ionic liquid (IL) and bio-renewable sources as chitosan (CHT) and chondroitin sulfate (CS) were used to yield hydrogel-based materials (CHT/CS).
28522008	1	23	theme	chondroitin	157:167	arg1	sulfate					169:175	chondroitin sulfate	157:175	chondroitin sulfate (CS)	157:180	[Hmim][HSO4] ionic liquid (IL) and bio-renewable sources as chitosan (CHT) and chondroitin sulfate (CS) were used to yield hydrogel-based materials (CHT/CS).
28522008	2	24	theme	green	328:332	arg1	principle					345:353	"green chemistry" principle	327:353	"green chemistry" principle	327:353	The use of IL to solubilize both polysaccharides was considered an innovative way based on "green chemistry" principle, aiming the production of CHT/CS blended systems.
28522008	2	25	theme	CHT/CS	381:386	arg1	systems					396:402	CHT/CS blended systems	381:402	CHT/CS blended systems	381:402	The use of IL to solubilize both polysaccharides was considered an innovative way based on "green chemistry" principle, aiming the production of CHT/CS blended systems.
28522008	4	26	theme	normal	663:668	arg1	VERO					670:673	the normal VERO and diseased HT29 cells	659:697	VERO	670:673	The hydrogels were characterized and achieved with excellent stabilities (in the 1.2-10pH range), larger swelling capacities, as well as devoid of cytotoxicity towards the normal VERO and diseased HT29 cells.
28522008	1	27	used	used	187:190	arg2	sources					127:133	[Hmim][HSO4] ionic liquid (IL) and bio-renewable sources	78:133	[Hmim][HSO4] ionic liquid (IL) and bio-renewable sources as chitosan (CHT) and chondroitin sulfate (CS)	78:180	[Hmim][HSO4] ionic liquid (IL) and bio-renewable sources as chitosan (CHT) and chondroitin sulfate (CS) were used to yield hydrogel-based materials (CHT/CS).
28522008	1	28	theme	ionic	91:95	arg1	sources					127:133	[Hmim][HSO4] ionic liquid (IL) and bio-renewable sources	78:133	[Hmim][HSO4] ionic liquid (IL) and bio-renewable sources as chitosan (CHT) and chondroitin sulfate (CS)	78:180	[Hmim][HSO4] ionic liquid (IL) and bio-renewable sources as chitosan (CHT) and chondroitin sulfate (CS) were used to yield hydrogel-based materials (CHT/CS).
28522008	5	29	theme	CHT/CS	704:709	arg1	hydrogels					711:719	The CHT/CS hydrogels	700:719	The CHT/CS hydrogels carried out in [Hmim][HSO4]	700:747	The CHT/CS hydrogels carried out in [Hmim][HSO4] could be applied in many technological purposes, like medical, pharmaceutical, and environmental fields.
28522008	5	30	theme	medical	803:809	arg1	fields					846:851	medical, pharmaceutical, and environmental fields	803:851	medical, pharmaceutical, and environmental fields	803:851	The CHT/CS hydrogels carried out in [Hmim][HSO4] could be applied in many technological purposes, like medical, pharmaceutical, and environmental fields.
28522008	3	31	from	times	484:488	arg1	medium					454:459	homogeneous medium	442:459	homogeneous medium from short dissolution times	442:488	CHT/CS hydrogels were carried out in homogeneous medium from short dissolution times.
28522008	4	32	theme	HT29	688:691	arg1	cells					693:697	the normal VERO and diseased HT29 cells	659:697	cells	693:697	The hydrogels were characterized and achieved with excellent stabilities (in the 1.2-10pH range), larger swelling capacities, as well as devoid of cytotoxicity towards the normal VERO and diseased HT29 cells.
28522008	2	33	theme	IL	247:248	arg1	use					240:242	The use	236:242	The use of IL to solubilize both polysaccharides	236:283	The use of IL to solubilize both polysaccharides was considered an innovative way based on "green chemistry" principle, aiming the production of CHT/CS blended systems.
28522008	4	34	from	stabilities	552:562	arg1	range					581:585	the 1.2-10pH range	568:585	the 1.2-10pH range	568:585	The hydrogels were characterized and achieved with excellent stabilities (in the 1.2-10pH range), larger swelling capacities, as well as devoid of cytotoxicity towards the normal VERO and diseased HT29 cells.
28522008	5	35	theme	many	769:772	arg1	purposes					788:795	many technological purposes	769:795	many technological purposes	769:795	The CHT/CS hydrogels carried out in [Hmim][HSO4] could be applied in many technological purposes, like medical, pharmaceutical, and environmental fields.
28522008	4	36	theme	swelling	596:603	arg1	capacities					605:614	larger swelling capacities	589:614	larger swelling capacities	589:614	The hydrogels were characterized and achieved with excellent stabilities (in the 1.2-10pH range), larger swelling capacities, as well as devoid of cytotoxicity towards the normal VERO and diseased HT29 cells.
28522008	4	37	theme	excellent	542:550	arg1	stabilities					552:562	excellent stabilities	542:562	excellent stabilities (in the 1.2-10pH range)	542:586	The hydrogels were characterized and achieved with excellent stabilities (in the 1.2-10pH range), larger swelling capacities, as well as devoid of cytotoxicity towards the normal VERO and diseased HT29 cells.
27185147	0	0	theme	antibacterial	81:93	arg1	applications					95:106	antibacterial applications	81:106	antibacterial applications	81:106	Fabrication of bacterial cellulose-ZnO composite via solution plasma process for antibacterial applications.
27185147	2	1	theme	splendid	487:494	arg1	pellicle					454:461	the BC pellicle	447:461	the BC pellicle	447:461	By the reasons of its nano-sized structure as well as favorable porous configuration, the BC pellicle has been proved to be a splendid upholding template for the coordination of ZnO.
27185147	2	1	theme	splendid	487:494	arg1	template					506:513	a splendid upholding template	485:513	a splendid upholding template for the coordination of ZnO	485:541	By the reasons of its nano-sized structure as well as favorable porous configuration, the BC pellicle has been proved to be a splendid upholding template for the coordination of ZnO.
27185147	4	2	theme	antibacterial	780:792	arg1	material					794:801	an antibacterial material	777:801	an antibacterial material in wound dressing and water disinfection applications	777:855	Hence, the ZnO-deposited BC composites can be used as an antibacterial material in wound dressing and water disinfection applications.
27185147	4	2	theme	antibacterial	780:792	arg1	composites					751:760	the ZnO-deposited BC composites	730:760	the ZnO-deposited BC composites	730:760	Hence, the ZnO-deposited BC composites can be used as an antibacterial material in wound dressing and water disinfection applications.
27185147	4	3	theme	disinfection	831:842	arg1	applications					844:855	water disinfection applications	825:855	water disinfection applications	825:855	Hence, the ZnO-deposited BC composites can be used as an antibacterial material in wound dressing and water disinfection applications.
27185147	1	4	theme	solution	169:176	arg1	discharge					195:203	a plasma discharge	186:203	a plasma discharge in a liquid phase	186:221	Zinc oxide (ZnO) was successfully synthesized by applying a solution plasma, a plasma discharge in a liquid phase, without the addition of a reducing agent and simultaneously deposited into a bacterial cellulose pellicle that functioned as a template.
27185147	1	4	theme	solution	169:176	arg1	plasma					178:183	a solution plasma	167:183	a solution plasma	167:183	Zinc oxide (ZnO) was successfully synthesized by applying a solution plasma, a plasma discharge in a liquid phase, without the addition of a reducing agent and simultaneously deposited into a bacterial cellulose pellicle that functioned as a template.
27185147	0	5	theme	solution	53:60	arg1	process					69:75	solution plasma process	53:75	solution plasma process for antibacterial applications	53:106	Fabrication of bacterial cellulose-ZnO composite via solution plasma process for antibacterial applications.
27185147	4	6	theme	water	825:829	arg1	applications					844:855	water disinfection applications	825:855	water disinfection applications	825:855	Hence, the ZnO-deposited BC composites can be used as an antibacterial material in wound dressing and water disinfection applications.
27185147	2	7	theme	porous	425:430	arg1	configuration					432:444	favorable porous configuration	415:444	its nano-sized structure as well as favorable porous configuration	379:444	By the reasons of its nano-sized structure as well as favorable porous configuration, the BC pellicle has been proved to be a splendid upholding template for the coordination of ZnO.
27185147	2	8	theme	favorable	415:423	arg1	configuration					432:444	favorable porous configuration	415:444	its nano-sized structure as well as favorable porous configuration	379:444	By the reasons of its nano-sized structure as well as favorable porous configuration, the BC pellicle has been proved to be a splendid upholding template for the coordination of ZnO.
27185147	1	9	theme	reducing	250:257	arg1	agent					259:263	a reducing agent	248:263	a reducing agent	248:263	Zinc oxide (ZnO) was successfully synthesized by applying a solution plasma, a plasma discharge in a liquid phase, without the addition of a reducing agent and simultaneously deposited into a bacterial cellulose pellicle that functioned as a template.
27185147	1	10	theme	Zinc	109:112	arg1	ZnO					121:123	ZnO	121:123	ZnO	121:123	Zinc oxide (ZnO) was successfully synthesized by applying a solution plasma, a plasma discharge in a liquid phase, without the addition of a reducing agent and simultaneously deposited into a bacterial cellulose pellicle that functioned as a template.
27185147	1	10	theme	Zinc	109:112	arg1	oxide					114:118	Zinc oxide	109:118	Zinc oxide (ZnO)	109:124	Zinc oxide (ZnO) was successfully synthesized by applying a solution plasma, a plasma discharge in a liquid phase, without the addition of a reducing agent and simultaneously deposited into a bacterial cellulose pellicle that functioned as a template.
27185147	0	11	theme	cellulose-ZnO	25:37	arg1	composite					39:47	bacterial cellulose-ZnO composite	15:47	bacterial cellulose-ZnO composite	15:47	Fabrication of bacterial cellulose-ZnO composite via solution plasma process for antibacterial applications.
27185147	1	12	theme	agent	259:263	arg1	addition					236:243	the addition	232:243	the addition of a reducing agent	232:263	Zinc oxide (ZnO) was successfully synthesized by applying a solution plasma, a plasma discharge in a liquid phase, without the addition of a reducing agent and simultaneously deposited into a bacterial cellulose pellicle that functioned as a template.
27185147	4	13	theme	BC	748:749	arg1	material					794:801	an antibacterial material	777:801	an antibacterial material in wound dressing and water disinfection applications	777:855	Hence, the ZnO-deposited BC composites can be used as an antibacterial material in wound dressing and water disinfection applications.
27185147	4	13	theme	BC	748:749	arg1	composites					751:760	the ZnO-deposited BC composites	730:760	the ZnO-deposited BC composites	730:760	Hence, the ZnO-deposited BC composites can be used as an antibacterial material in wound dressing and water disinfection applications.
27185147	1	14	theme	plasma	188:193	arg1	discharge					195:203	a plasma discharge	186:203	a plasma discharge in a liquid phase	186:221	Zinc oxide (ZnO) was successfully synthesized by applying a solution plasma, a plasma discharge in a liquid phase, without the addition of a reducing agent and simultaneously deposited into a bacterial cellulose pellicle that functioned as a template.
27185147	1	14	theme	plasma	188:193	arg1	plasma					178:183	a solution plasma	167:183	a solution plasma	167:183	Zinc oxide (ZnO) was successfully synthesized by applying a solution plasma, a plasma discharge in a liquid phase, without the addition of a reducing agent and simultaneously deposited into a bacterial cellulose pellicle that functioned as a template.
27185147	0	15	theme	bacterial	15:23	arg1	composite					39:47	bacterial cellulose-ZnO composite	15:47	bacterial cellulose-ZnO composite	15:47	Fabrication of bacterial cellulose-ZnO composite via solution plasma process for antibacterial applications.
27185147	4	16	theme	ZnO-deposited	734:746	arg1	material					794:801	an antibacterial material	777:801	an antibacterial material in wound dressing and water disinfection applications	777:855	Hence, the ZnO-deposited BC composites can be used as an antibacterial material in wound dressing and water disinfection applications.
27185147	4	16	theme	ZnO-deposited	734:746	arg1	composites					751:760	the ZnO-deposited BC composites	730:760	the ZnO-deposited BC composites	730:760	Hence, the ZnO-deposited BC composites can be used as an antibacterial material in wound dressing and water disinfection applications.
27185147	2	17	theme	structure	394:402	arg1	reasons					368:374	the reasons	364:374	the reasons of its nano-sized structure as well as favorable porous configuration	364:444	By the reasons of its nano-sized structure as well as favorable porous configuration, the BC pellicle has been proved to be a splendid upholding template for the coordination of ZnO.
27185147	2	18	theme	BC	451:452	arg1	pellicle					454:461	the BC pellicle	447:461	the BC pellicle	447:461	By the reasons of its nano-sized structure as well as favorable porous configuration, the BC pellicle has been proved to be a splendid upholding template for the coordination of ZnO.
27185147	2	18	theme	BC	451:452	arg1	template					506:513	a splendid upholding template	485:513	a splendid upholding template for the coordination of ZnO	485:541	By the reasons of its nano-sized structure as well as favorable porous configuration, the BC pellicle has been proved to be a splendid upholding template for the coordination of ZnO.
27185147	4	19	theme	wound	806:810	arg1	dressing					812:819	wound dressing	806:819	wound dressing	806:819	Hence, the ZnO-deposited BC composites can be used as an antibacterial material in wound dressing and water disinfection applications.
27185147	2	20	theme	nano-sized	383:392	arg1	structure					394:402	its nano-sized structure	379:402	its nano-sized structure as well as favorable porous configuration	379:444	By the reasons of its nano-sized structure as well as favorable porous configuration, the BC pellicle has been proved to be a splendid upholding template for the coordination of ZnO.
27185147	0	21	theme	composite	39:47	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of bacterial cellulose-ZnO composite via solution plasma process for antibacterial applications.	0:107	Fabrication of bacterial cellulose-ZnO composite via solution plasma process for antibacterial applications.
27185147	2	22	theme	upholding	496:504	arg1	pellicle					454:461	the BC pellicle	447:461	the BC pellicle	447:461	By the reasons of its nano-sized structure as well as favorable porous configuration, the BC pellicle has been proved to be a splendid upholding template for the coordination of ZnO.
27185147	2	22	theme	upholding	496:504	arg1	template					506:513	a splendid upholding template	485:513	a splendid upholding template for the coordination of ZnO	485:541	By the reasons of its nano-sized structure as well as favorable porous configuration, the BC pellicle has been proved to be a splendid upholding template for the coordination of ZnO.
27185147	3	23	theme	strong	602:607	arg1	activity					623:630	strong antibacterial activity	602:630	strong antibacterial activity	602:630	In addition, the ZnO-deposited BC composites demonstrated strong antibacterial activity without a photocatalytic reaction against both Staphylococcus aureus and Escherichia coli.
27185147	0	24	theme	plasma	62:67	arg1	process					69:75	solution plasma process	53:75	solution plasma process for antibacterial applications	53:106	Fabrication of bacterial cellulose-ZnO composite via solution plasma process for antibacterial applications.
27185147	3	25	theme	antibacterial	609:621	arg1	activity					623:630	strong antibacterial activity	602:630	strong antibacterial activity	602:630	In addition, the ZnO-deposited BC composites demonstrated strong antibacterial activity without a photocatalytic reaction against both Staphylococcus aureus and Escherichia coli.
27185147	2	26	theme	configuration	432:444	arg1	reasons					368:374	the reasons	364:374	the reasons of its nano-sized structure as well as favorable porous configuration	364:444	By the reasons of its nano-sized structure as well as favorable porous configuration, the BC pellicle has been proved to be a splendid upholding template for the coordination of ZnO.
27185147	2	27	theme	ZnO	539:541	arg1	coordination					523:534	the coordination	519:534	the coordination of ZnO	519:541	By the reasons of its nano-sized structure as well as favorable porous configuration, the BC pellicle has been proved to be a splendid upholding template for the coordination of ZnO.
27185147	1	28	theme	bacterial	301:309	arg1	template					351:358	a template	349:358	a template	349:358	Zinc oxide (ZnO) was successfully synthesized by applying a solution plasma, a plasma discharge in a liquid phase, without the addition of a reducing agent and simultaneously deposited into a bacterial cellulose pellicle that functioned as a template.
27185147	1	28	theme	bacterial	301:309	arg1	pellicle					321:328	a bacterial cellulose pellicle	299:328	a bacterial cellulose pellicle that functioned as a template	299:358	Zinc oxide (ZnO) was successfully synthesized by applying a solution plasma, a plasma discharge in a liquid phase, without the addition of a reducing agent and simultaneously deposited into a bacterial cellulose pellicle that functioned as a template.
27185147	1	29	from	discharge	195:203	arg1	phase					217:221	a liquid phase	208:221	a liquid phase	208:221	Zinc oxide (ZnO) was successfully synthesized by applying a solution plasma, a plasma discharge in a liquid phase, without the addition of a reducing agent and simultaneously deposited into a bacterial cellulose pellicle that functioned as a template.
27185147	4	30	used	used	769:772	arg2	material					794:801	an antibacterial material	777:801	an antibacterial material in wound dressing and water disinfection applications	777:855	Hence, the ZnO-deposited BC composites can be used as an antibacterial material in wound dressing and water disinfection applications.
27185147	4	30	used	used	769:772	arg2	composites					751:760	the ZnO-deposited BC composites	730:760	the ZnO-deposited BC composites	730:760	Hence, the ZnO-deposited BC composites can be used as an antibacterial material in wound dressing and water disinfection applications.
27185147	1	31	theme	liquid	210:215	arg1	phase					217:221	a liquid phase	208:221	a liquid phase	208:221	Zinc oxide (ZnO) was successfully synthesized by applying a solution plasma, a plasma discharge in a liquid phase, without the addition of a reducing agent and simultaneously deposited into a bacterial cellulose pellicle that functioned as a template.
27185147	4	32	from	material	794:801	arg1	applications					844:855	water disinfection applications	825:855	water disinfection applications	825:855	Hence, the ZnO-deposited BC composites can be used as an antibacterial material in wound dressing and water disinfection applications.
27185147	4	32	from	material	794:801	arg1	dressing					812:819	wound dressing	806:819	wound dressing	806:819	Hence, the ZnO-deposited BC composites can be used as an antibacterial material in wound dressing and water disinfection applications.
27185147	3	33	theme	photocatalytic	642:655	arg1	reaction					657:664	a photocatalytic reaction	640:664	a photocatalytic reaction against both Staphylococcus aureus and Escherichia coli	640:720	In addition, the ZnO-deposited BC composites demonstrated strong antibacterial activity without a photocatalytic reaction against both Staphylococcus aureus and Escherichia coli.
27185147	3	34	theme	ZnO-deposited	561:573	arg1	composites					578:587	the ZnO-deposited BC composites	557:587	the ZnO-deposited BC composites	557:587	In addition, the ZnO-deposited BC composites demonstrated strong antibacterial activity without a photocatalytic reaction against both Staphylococcus aureus and Escherichia coli.
27185147	1	35	theme	cellulose	311:319	arg1	template					351:358	a template	349:358	a template	349:358	Zinc oxide (ZnO) was successfully synthesized by applying a solution plasma, a plasma discharge in a liquid phase, without the addition of a reducing agent and simultaneously deposited into a bacterial cellulose pellicle that functioned as a template.
27185147	1	35	theme	cellulose	311:319	arg1	pellicle					321:328	a bacterial cellulose pellicle	299:328	a bacterial cellulose pellicle that functioned as a template	299:358	Zinc oxide (ZnO) was successfully synthesized by applying a solution plasma, a plasma discharge in a liquid phase, without the addition of a reducing agent and simultaneously deposited into a bacterial cellulose pellicle that functioned as a template.
27185147	3	36	theme	BC	575:576	arg1	composites					578:587	the ZnO-deposited BC composites	557:587	the ZnO-deposited BC composites	557:587	In addition, the ZnO-deposited BC composites demonstrated strong antibacterial activity without a photocatalytic reaction against both Staphylococcus aureus and Escherichia coli.
25844452	4	0	theme	identical	971:979	arg1	structure					981:989	identical structure	971:989	identical structure	971:989	High degree of serological similarity between the strains under study was shown to result from the presence of repeating units with identical structure in their O antigens.
25844452	3	1	theme	methylation	697:707	arg1	analysis					709:716	methylation analysis	697:716	methylation analysis	697:716	Monosaccharide analysis of the O-specific polysaccharides (including determination of the absolute configurations, methylation analysis, and one- and two-dimensional NMR spectroscopy) revealed the presence of two types of repeating units in varying ratios.
25844452	2	2	theme	acids	575:579	arg1	predominance					495:506	predominance	495:506	predominance of 3-hydroxytetradecanoic, 3-hydroxyhexadecanoic, and octadecenoic acids	495:579	In the lipid A fatty acid composition, the lipopolysaccharides under study were similar to those of other Azospirillum strains and were characterized by predominance of 3-hydroxytetradecanoic, 3-hydroxyhexadecanoic, and octadecenoic acids.
25844452	3	3	theme	absolute	672:679	arg1	configurations					681:694	the absolute configurations	668:694	the absolute configurations	668:694	Monosaccharide analysis of the O-specific polysaccharides (including determination of the absolute configurations, methylation analysis, and one- and two-dimensional NMR spectroscopy) revealed the presence of two types of repeating units in varying ratios.
25844452	2	4	theme	octadecenoic	562:573	arg1	acids					575:579	3-hydroxytetradecanoic, 3-hydroxyhexadecanoic, and octadecenoic acids	511:579	3-hydroxytetradecanoic, 3-hydroxyhexadecanoic, and octadecenoic acids	511:579	In the lipid A fatty acid composition, the lipopolysaccharides under study were similar to those of other Azospirillum strains and were characterized by predominance of 3-hydroxytetradecanoic, 3-hydroxyhexadecanoic, and octadecenoic acids.
25844452	4	5	theme	similarity	866:875	arg1	degree					844:849	High degree	839:849	High degree of serological similarity between the strains under study	839:907	High degree of serological similarity between the strains under study was shown to result from the presence of repeating units with identical structure in their O antigens.
25844452	3	6	theme	varying	823:829	arg1	ratios					831:836	varying ratios	823:836	varying ratios	823:836	Monosaccharide analysis of the O-specific polysaccharides (including determination of the absolute configurations, methylation analysis, and one- and two-dimensional NMR spectroscopy) revealed the presence of two types of repeating units in varying ratios.
25844452	3	7	theme	configurations	681:694	arg1	determination					651:663	determination	651:663	determination of the absolute configurations	651:694	Monosaccharide analysis of the O-specific polysaccharides (including determination of the absolute configurations, methylation analysis, and one- and two-dimensional NMR spectroscopy) revealed the presence of two types of repeating units in varying ratios.
25844452	3	7	theme	configurations	681:694	arg1	spectroscopy					752:763	one- and two-dimensional NMR spectroscopy	723:763	one- and two-dimensional NMR spectroscopy	723:763	Monosaccharide analysis of the O-specific polysaccharides (including determination of the absolute configurations, methylation analysis, and one- and two-dimensional NMR spectroscopy) revealed the presence of two types of repeating units in varying ratios.
25844452	3	7	theme	configurations	681:694	arg1	analysis					709:716	methylation analysis	697:716	methylation analysis	697:716	Monosaccharide analysis of the O-specific polysaccharides (including determination of the absolute configurations, methylation analysis, and one- and two-dimensional NMR spectroscopy) revealed the presence of two types of repeating units in varying ratios.
25844452	3	8	theme	repeating	804:812	arg1	units					814:818	repeating units	804:818	repeating units	804:818	Monosaccharide analysis of the O-specific polysaccharides (including determination of the absolute configurations, methylation analysis, and one- and two-dimensional NMR spectroscopy) revealed the presence of two types of repeating units in varying ratios.
25844452	3	9	from	presence	779:786	arg1	ratios					831:836	varying ratios	823:836	varying ratios	823:836	Monosaccharide analysis of the O-specific polysaccharides (including determination of the absolute configurations, methylation analysis, and one- and two-dimensional NMR spectroscopy) revealed the presence of two types of repeating units in varying ratios.
25844452	3	10	theme	units	814:818	arg1	types					795:799	two types	791:799	two types of repeating units	791:818	Monosaccharide analysis of the O-specific polysaccharides (including determination of the absolute configurations, methylation analysis, and one- and two-dimensional NMR spectroscopy) revealed the presence of two types of repeating units in varying ratios.
25844452	2	11	theme	Azospirillum	448:459	arg1	strains					461:467	other Azospirillum strains	442:467	other Azospirillum strains	442:467	In the lipid A fatty acid composition, the lipopolysaccharides under study were similar to those of other Azospirillum strains and were characterized by predominance of 3-hydroxytetradecanoic, 3-hydroxyhexadecanoic, and octadecenoic acids.
25844452	3	12	theme	one-	723:726	arg1	spectroscopy					752:763	one- and two-dimensional NMR spectroscopy	723:763	one- and two-dimensional NMR spectroscopy	723:763	Monosaccharide analysis of the O-specific polysaccharides (including determination of the absolute configurations, methylation analysis, and one- and two-dimensional NMR spectroscopy) revealed the presence of two types of repeating units in varying ratios.
25844452	4	13	theme	serological	854:864	arg1	similarity					866:875	serological similarity	854:875	serological similarity between the strains under study	854:907	High degree of serological similarity between the strains under study was shown to result from the presence of repeating units with identical structure in their O antigens.
25844452	2	14	theme	other	442:446	arg1	strains					461:467	other Azospirillum strains	442:467	other Azospirillum strains	442:467	In the lipid A fatty acid composition, the lipopolysaccharides under study were similar to those of other Azospirillum strains and were characterized by predominance of 3-hydroxytetradecanoic, 3-hydroxyhexadecanoic, and octadecenoic acids.
25844452	1	15	theme	serological	307:317	arg1	analysis					319:326	serological analysis	307:326	serological analysis	307:326	Lipopolysaccharides of six Azospirillum strains (A. brasilense SR50, SR80, SR88, SR109, SR111, SR115, and A. lipoferum SR 42) isolated from the rhizosphere of cereal plants of Saratov oblast, Russia and assigned to serogroup II by serological analysis were studied.
25844452	1	16	dep	brasilense	128:137	arg1	lipoferum					185:193	lipoferum SR 42	185:199	A. brasilense SR50, SR80, SR88, SR109, SR111, SR115, and A. lipoferum SR 42	125:199	Lipopolysaccharides of six Azospirillum strains (A. brasilense SR50, SR80, SR88, SR109, SR111, SR115, and A. lipoferum SR 42) isolated from the rhizosphere of cereal plants of Saratov oblast, Russia and assigned to serogroup II by serological analysis were studied.
25844452	3	17	theme	NMR	748:750	arg1	spectroscopy					752:763	one- and two-dimensional NMR spectroscopy	723:763	one- and two-dimensional NMR spectroscopy	723:763	Monosaccharide analysis of the O-specific polysaccharides (including determination of the absolute configurations, methylation analysis, and one- and two-dimensional NMR spectroscopy) revealed the presence of two types of repeating units in varying ratios.
25844452	4	18	theme	High	839:842	arg1	degree					844:849	High degree	839:849	High degree of serological similarity between the strains under study	839:907	High degree of serological similarity between the strains under study was shown to result from the presence of repeating units with identical structure in their O antigens.
25844452	1	19	theme	Azospirillum	103:114	arg1	SR109					157:161	SR109	157:161	SR109	157:161	Lipopolysaccharides of six Azospirillum strains (A. brasilense SR50, SR80, SR88, SR109, SR111, SR115, and A. lipoferum SR 42) isolated from the rhizosphere of cereal plants of Saratov oblast, Russia and assigned to serogroup II by serological analysis were studied.
25844452	1	19	theme	Azospirillum	103:114	arg1	strains					116:122	six Azospirillum strains	99:122	six Azospirillum strains (A. brasilense SR50, SR80, SR88, SR109, SR111, SR115, and A. lipoferum SR 42) isolated from the rhizosphere of cereal plants of Saratov oblast, Russia and assigned to serogroup II by serological analysis	99:326	Lipopolysaccharides of six Azospirillum strains (A. brasilense SR50, SR80, SR88, SR109, SR111, SR115, and A. lipoferum SR 42) isolated from the rhizosphere of cereal plants of Saratov oblast, Russia and assigned to serogroup II by serological analysis were studied.
25844452	1	19	theme	Azospirillum	103:114	arg1	brasilense					128:137	brasilense	128:137	brasilense	128:137	Lipopolysaccharides of six Azospirillum strains (A. brasilense SR50, SR80, SR88, SR109, SR111, SR115, and A. lipoferum SR 42) isolated from the rhizosphere of cereal plants of Saratov oblast, Russia and assigned to serogroup II by serological analysis were studied.
25844452	1	19	theme	Azospirillum	103:114	arg1	SR115					171:175	SR115	171:175	SR115	171:175	Lipopolysaccharides of six Azospirillum strains (A. brasilense SR50, SR80, SR88, SR109, SR111, SR115, and A. lipoferum SR 42) isolated from the rhizosphere of cereal plants of Saratov oblast, Russia and assigned to serogroup II by serological analysis were studied.
25844452	1	19	theme	Azospirillum	103:114	arg1	A.					182:183	A.	182:183	A.	182:183	Lipopolysaccharides of six Azospirillum strains (A. brasilense SR50, SR80, SR88, SR109, SR111, SR115, and A. lipoferum SR 42) isolated from the rhizosphere of cereal plants of Saratov oblast, Russia and assigned to serogroup II by serological analysis were studied.
25844452	0	20	theme	lipopolysaccharides	25:43	arg1	[Characterization					0:16	[Characterization	0:16	[Characterization of the lipopolysaccharides of serogroup II Azospirillum	0:72	[Characterization of the lipopolysaccharides of serogroup II Azospirillum].
25844452	1	21	theme	cereal	235:240	arg1	plants					242:247	cereal plants	235:247	cereal plants of Saratov oblast, Russia	235:273	Lipopolysaccharides of six Azospirillum strains (A. brasilense SR50, SR80, SR88, SR109, SR111, SR115, and A. lipoferum SR 42) isolated from the rhizosphere of cereal plants of Saratov oblast, Russia and assigned to serogroup II by serological analysis were studied.
25844452	3	22	attach	presence	779:786	arg1	ratios					831:836	varying ratios	823:836	varying ratios	823:836	Monosaccharide analysis of the O-specific polysaccharides (including determination of the absolute configurations, methylation analysis, and one- and two-dimensional NMR spectroscopy) revealed the presence of two types of repeating units in varying ratios.
25844452	3	22	attach	presence	779:786	arg2	types					795:799	two types	791:799	two types of repeating units	791:818	Monosaccharide analysis of the O-specific polysaccharides (including determination of the absolute configurations, methylation analysis, and one- and two-dimensional NMR spectroscopy) revealed the presence of two types of repeating units in varying ratios.
25844452	1	23	theme	strains	116:122	arg1	Lipopolysaccharides					76:94	Lipopolysaccharides	76:94	Lipopolysaccharides of six Azospirillum strains (A. brasilense SR50, SR80, SR88, SR109, SR111, SR115, and A. lipoferum SR 42) isolated from the rhizosphere of cereal plants of Saratov oblast, Russia and assigned to serogroup II by serological analysis	76:326	Lipopolysaccharides of six Azospirillum strains (A. brasilense SR50, SR80, SR88, SR109, SR111, SR115, and A. lipoferum SR 42) isolated from the rhizosphere of cereal plants of Saratov oblast, Russia and assigned to serogroup II by serological analysis were studied.
25844452	2	24	theme	fatty	357:361	arg1	composition					368:378	the lipid A fatty acid composition	345:378	the lipid A fatty acid composition	345:378	In the lipid A fatty acid composition, the lipopolysaccharides under study were similar to those of other Azospirillum strains and were characterized by predominance of 3-hydroxytetradecanoic, 3-hydroxyhexadecanoic, and octadecenoic acids.
25844452	2	25	theme	acid	363:366	arg1	composition					368:378	the lipid A fatty acid composition	345:378	the lipid A fatty acid composition	345:378	In the lipid A fatty acid composition, the lipopolysaccharides under study were similar to those of other Azospirillum strains and were characterized by predominance of 3-hydroxytetradecanoic, 3-hydroxyhexadecanoic, and octadecenoic acids.
25844452	1	26	theme	plants	242:247	arg1	rhizosphere					220:230	the rhizosphere	216:230	the rhizosphere of cereal plants of Saratov oblast, Russia	216:273	Lipopolysaccharides of six Azospirillum strains (A. brasilense SR50, SR80, SR88, SR109, SR111, SR115, and A. lipoferum SR 42) isolated from the rhizosphere of cereal plants of Saratov oblast, Russia and assigned to serogroup II by serological analysis were studied.
25844452	3	27	theme	polysaccharides	624:638	arg1	analysis					597:604	Monosaccharide analysis	582:604	Monosaccharide analysis of the O-specific polysaccharides (including determination of the absolute configurations, methylation analysis, and one- and two-dimensional NMR spectroscopy)	582:764	Monosaccharide analysis of the O-specific polysaccharides (including determination of the absolute configurations, methylation analysis, and one- and two-dimensional NMR spectroscopy) revealed the presence of two types of repeating units in varying ratios.
25844452	2	28	theme	A	355:355	arg1	composition					368:378	the lipid A fatty acid composition	345:378	the lipid A fatty acid composition	345:378	In the lipid A fatty acid composition, the lipopolysaccharides under study were similar to those of other Azospirillum strains and were characterized by predominance of 3-hydroxytetradecanoic, 3-hydroxyhexadecanoic, and octadecenoic acids.
25844452	0	29	theme	serogroup	48:56	arg1	Azospirillum					61:72	serogroup II Azospirillum	48:72	serogroup II Azospirillum	48:72	[Characterization of the lipopolysaccharides of serogroup II Azospirillum].
25844452	3	30	theme	two-dimensional	732:746	arg1	spectroscopy					752:763	one- and two-dimensional NMR spectroscopy	723:763	one- and two-dimensional NMR spectroscopy	723:763	Monosaccharide analysis of the O-specific polysaccharides (including determination of the absolute configurations, methylation analysis, and one- and two-dimensional NMR spectroscopy) revealed the presence of two types of repeating units in varying ratios.
25844452	2	31	from	similar	422:428	arg1	composition					368:378	the lipid A fatty acid composition	345:378	the lipid A fatty acid composition	345:378	In the lipid A fatty acid composition, the lipopolysaccharides under study were similar to those of other Azospirillum strains and were characterized by predominance of 3-hydroxytetradecanoic, 3-hydroxyhexadecanoic, and octadecenoic acids.
25844452	2	32	theme	lipid	349:353	arg1	composition					368:378	the lipid A fatty acid composition	345:378	the lipid A fatty acid composition	345:378	In the lipid A fatty acid composition, the lipopolysaccharides under study were similar to those of other Azospirillum strains and were characterized by predominance of 3-hydroxytetradecanoic, 3-hydroxyhexadecanoic, and octadecenoic acids.
25844452	1	33	theme	Saratov	252:258	arg1	oblast					260:265	Saratov oblast	252:265	Saratov oblast	252:265	Lipopolysaccharides of six Azospirillum strains (A. brasilense SR50, SR80, SR88, SR109, SR111, SR115, and A. lipoferum SR 42) isolated from the rhizosphere of cereal plants of Saratov oblast, Russia and assigned to serogroup II by serological analysis were studied.
25844452	1	33	theme	Saratov	252:258	arg1	Russia					268:273	Russia	268:273	Russia	268:273	Lipopolysaccharides of six Azospirillum strains (A. brasilense SR50, SR80, SR88, SR109, SR111, SR115, and A. lipoferum SR 42) isolated from the rhizosphere of cereal plants of Saratov oblast, Russia and assigned to serogroup II by serological analysis were studied.
25844452	4	34	from	presence	938:945	arg1	antigens					1002:1009	their O antigens	994:1009	their O antigens	994:1009	High degree of serological similarity between the strains under study was shown to result from the presence of repeating units with identical structure in their O antigens.
25844452	0	35	theme	Azospirillum	61:72	arg1	lipopolysaccharides					25:43	the lipopolysaccharides	21:43	the lipopolysaccharides of serogroup II Azospirillum	21:72	[Characterization of the lipopolysaccharides of serogroup II Azospirillum].
25844452	1	36	theme	oblast	260:265	arg1	plants					242:247	cereal plants	235:247	cereal plants of Saratov oblast, Russia	235:273	Lipopolysaccharides of six Azospirillum strains (A. brasilense SR50, SR80, SR88, SR109, SR111, SR115, and A. lipoferum SR 42) isolated from the rhizosphere of cereal plants of Saratov oblast, Russia and assigned to serogroup II by serological analysis were studied.
25844452	2	37	theme	3-hydroxyhexadecanoic	535:555	arg1	acids					575:579	3-hydroxytetradecanoic, 3-hydroxyhexadecanoic, and octadecenoic acids	511:579	3-hydroxytetradecanoic, 3-hydroxyhexadecanoic, and octadecenoic acids	511:579	In the lipid A fatty acid composition, the lipopolysaccharides under study were similar to those of other Azospirillum strains and were characterized by predominance of 3-hydroxytetradecanoic, 3-hydroxyhexadecanoic, and octadecenoic acids.
25844452	3	38	theme	O-specific	613:622	arg1	polysaccharides					624:638	the O-specific polysaccharides	609:638	the O-specific polysaccharides (including determination of the absolute configurations, methylation analysis, and one- and two-dimensional NMR spectroscopy)	609:764	Monosaccharide analysis of the O-specific polysaccharides (including determination of the absolute configurations, methylation analysis, and one- and two-dimensional NMR spectroscopy) revealed the presence of two types of repeating units in varying ratios.
25844452	3	38	theme	O-specific	613:622	arg1	determination					651:663	determination	651:663	determination of the absolute configurations	651:694	Monosaccharide analysis of the O-specific polysaccharides (including determination of the absolute configurations, methylation analysis, and one- and two-dimensional NMR spectroscopy) revealed the presence of two types of repeating units in varying ratios.
25844452	3	38	theme	O-specific	613:622	arg1	spectroscopy					752:763	one- and two-dimensional NMR spectroscopy	723:763	one- and two-dimensional NMR spectroscopy	723:763	Monosaccharide analysis of the O-specific polysaccharides (including determination of the absolute configurations, methylation analysis, and one- and two-dimensional NMR spectroscopy) revealed the presence of two types of repeating units in varying ratios.
25844452	3	38	theme	O-specific	613:622	arg1	analysis					709:716	methylation analysis	697:716	methylation analysis	697:716	Monosaccharide analysis of the O-specific polysaccharides (including determination of the absolute configurations, methylation analysis, and one- and two-dimensional NMR spectroscopy) revealed the presence of two types of repeating units in varying ratios.
25844452	4	39	theme	repeating	950:958	arg1	units					960:964	repeating units	950:964	repeating units with identical structure	950:989	High degree of serological similarity between the strains under study was shown to result from the presence of repeating units with identical structure in their O antigens.
25844452	4	40	with	units	960:964	arg1	structure					981:989	identical structure	971:989	identical structure	971:989	High degree of serological similarity between the strains under study was shown to result from the presence of repeating units with identical structure in their O antigens.
25844452	2	41	theme	3-hydroxytetradecanoic	511:532	arg1	acids					575:579	3-hydroxytetradecanoic, 3-hydroxyhexadecanoic, and octadecenoic acids	511:579	3-hydroxytetradecanoic, 3-hydroxyhexadecanoic, and octadecenoic acids	511:579	In the lipid A fatty acid composition, the lipopolysaccharides under study were similar to those of other Azospirillum strains and were characterized by predominance of 3-hydroxytetradecanoic, 3-hydroxyhexadecanoic, and octadecenoic acids.
25844452	3	42	theme	types	795:799	arg1	presence					779:786	the presence	775:786	the presence of two types of repeating units in varying ratios	775:836	Monosaccharide analysis of the O-specific polysaccharides (including determination of the absolute configurations, methylation analysis, and one- and two-dimensional NMR spectroscopy) revealed the presence of two types of repeating units in varying ratios.
25844452	1	43	theme	SR	195:196	arg1	lipoferum					185:193	lipoferum SR 42	185:199	A. brasilense SR50, SR80, SR88, SR109, SR111, SR115, and A. lipoferum SR 42	125:199	Lipopolysaccharides of six Azospirillum strains (A. brasilense SR50, SR80, SR88, SR109, SR111, SR115, and A. lipoferum SR 42) isolated from the rhizosphere of cereal plants of Saratov oblast, Russia and assigned to serogroup II by serological analysis were studied.
25844452	4	44	theme	units	960:964	arg1	presence					938:945	the presence	934:945	the presence of repeating units with identical structure in their O antigens	934:1009	High degree of serological similarity between the strains under study was shown to result from the presence of repeating units with identical structure in their O antigens.
25844452	1	45	dep	strains	116:122	arg1	strains					116:122	six Azospirillum strains	99:122	six Azospirillum strains (A. brasilense SR50, SR80, SR88, SR109, SR111, SR115, and A. lipoferum SR 42) isolated from the rhizosphere of cereal plants of Saratov oblast, Russia and assigned to serogroup II by serological analysis	99:326	Lipopolysaccharides of six Azospirillum strains (A. brasilense SR50, SR80, SR88, SR109, SR111, SR115, and A. lipoferum SR 42) isolated from the rhizosphere of cereal plants of Saratov oblast, Russia and assigned to serogroup II by serological analysis were studied.
25844452	1	45	dep	strains	116:122	arg1	SR115					171:175	SR115	171:175	SR115	171:175	Lipopolysaccharides of six Azospirillum strains (A. brasilense SR50, SR80, SR88, SR109, SR111, SR115, and A. lipoferum SR 42) isolated from the rhizosphere of cereal plants of Saratov oblast, Russia and assigned to serogroup II by serological analysis were studied.
25844452	1	45	dep	strains	116:122	arg1	assigned					279:286	assigned	279:286	assigned to serogroup II by serological analysis	279:326	Lipopolysaccharides of six Azospirillum strains (A. brasilense SR50, SR80, SR88, SR109, SR111, SR115, and A. lipoferum SR 42) isolated from the rhizosphere of cereal plants of Saratov oblast, Russia and assigned to serogroup II by serological analysis were studied.
25844452	1	45	dep	strains	116:122	arg1	isolated					202:209	isolated	202:209	isolated from the rhizosphere of cereal plants of Saratov oblast, Russia	202:273	Lipopolysaccharides of six Azospirillum strains (A. brasilense SR50, SR80, SR88, SR109, SR111, SR115, and A. lipoferum SR 42) isolated from the rhizosphere of cereal plants of Saratov oblast, Russia and assigned to serogroup II by serological analysis were studied.
25844452	1	45	dep	strains	116:122	arg1	A.					182:183	A.	182:183	A.	182:183	Lipopolysaccharides of six Azospirillum strains (A. brasilense SR50, SR80, SR88, SR109, SR111, SR115, and A. lipoferum SR 42) isolated from the rhizosphere of cereal plants of Saratov oblast, Russia and assigned to serogroup II by serological analysis were studied.
25844452	1	45	dep	strains	116:122	arg1	SR109					157:161	SR109	157:161	SR109	157:161	Lipopolysaccharides of six Azospirillum strains (A. brasilense SR50, SR80, SR88, SR109, SR111, SR115, and A. lipoferum SR 42) isolated from the rhizosphere of cereal plants of Saratov oblast, Russia and assigned to serogroup II by serological analysis were studied.
25844452	1	45	dep	strains	116:122	arg1	brasilense					128:137	brasilense	128:137	brasilense	128:137	Lipopolysaccharides of six Azospirillum strains (A. brasilense SR50, SR80, SR88, SR109, SR111, SR115, and A. lipoferum SR 42) isolated from the rhizosphere of cereal plants of Saratov oblast, Russia and assigned to serogroup II by serological analysis were studied.
25844452	4	46	theme	O	1000:1000	arg1	antigens					1002:1009	their O antigens	994:1009	their O antigens	994:1009	High degree of serological similarity between the strains under study was shown to result from the presence of repeating units with identical structure in their O antigens.
25844452	2	47	from	composition	368:378	arg1	similar					422:428	similar	422:428	similar	422:428	In the lipid A fatty acid composition, the lipopolysaccharides under study were similar to those of other Azospirillum strains and were characterized by predominance of 3-hydroxytetradecanoic, 3-hydroxyhexadecanoic, and octadecenoic acids.
25844452	2	47	from	composition	368:378	arg1	lipopolysaccharides					385:403	the lipopolysaccharides	381:403	the lipopolysaccharides under study	381:415	In the lipid A fatty acid composition, the lipopolysaccharides under study were similar to those of other Azospirillum strains and were characterized by predominance of 3-hydroxytetradecanoic, 3-hydroxyhexadecanoic, and octadecenoic acids.
25844452	3	48	theme	Monosaccharide	582:595	arg1	analysis					597:604	Monosaccharide analysis	582:604	Monosaccharide analysis of the O-specific polysaccharides (including determination of the absolute configurations, methylation analysis, and one- and two-dimensional NMR spectroscopy)	582:764	Monosaccharide analysis of the O-specific polysaccharides (including determination of the absolute configurations, methylation analysis, and one- and two-dimensional NMR spectroscopy) revealed the presence of two types of repeating units in varying ratios.
25844452	4	49	attach	presence	938:945	arg2	units					960:964	repeating units	950:964	repeating units with identical structure	950:989	High degree of serological similarity between the strains under study was shown to result from the presence of repeating units with identical structure in their O antigens.
25844452	4	49	attach	presence	938:945	arg1	antigens					1002:1009	their O antigens	994:1009	their O antigens	994:1009	High degree of serological similarity between the strains under study was shown to result from the presence of repeating units with identical structure in their O antigens.
28669623	4	0	theme	locust	619:624	arg1	formulation					641:651	a single locust bean gum (LBG) formulation	610:651	a single locust bean gum (LBG) formulation of microparticles associating both isoniazid and rifabutin	610:710	This work proposes inhalable therapy of pulmonary tuberculosis mediated by a single locust bean gum (LBG) formulation of microparticles associating both isoniazid and rifabutin, complying with requisites of the World Health Organisation of combined therapy.
28669623	6	1	theme	%	967:967	arg1	doses					946:950	emitted doses	938:950	emitted doses of more than 90%	938:967	The aerodynamic characterisation of microparticles revealed emitted doses of more than 90% and fine particle fraction of 38%, thus indicating the adequacy of the system to reach the respiratory lung area, thus partially the alveolar region.
28669623	6	1	theme	%	967:967	arg1	fraction					987:994	fine particle fraction	973:994	fine particle fraction of 38%	973:1001	The aerodynamic characterisation of microparticles revealed emitted doses of more than 90% and fine particle fraction of 38%, thus indicating the adequacy of the system to reach the respiratory lung area, thus partially the alveolar region.
28669623	8	2	theme	rat	1249:1251	arg1	macrophages					1262:1272	rat alveolar macrophages	1249:1272	rat alveolar macrophages	1249:1272	Additionally, rat alveolar macrophages evidenced preferential capture of LBG microparticles, possibly due to chemical composition comprising mannose and galactose units that are specifically recognised by macrophage surface receptors.
28669623	1	3	theme	deadly	162:167	arg1	pathology					169:177	a deadly pathology	160:177	a deadly pathology	160:177	Despite the existence of effective oral therapy, tuberculosis remains a deadly pathology, namely because of bacterial resistance and incompliance with treatments.
28669623	4	4	theme	pulmonary	575:583	arg1	tuberculosis					585:596	pulmonary tuberculosis	575:596	pulmonary tuberculosis mediated by a single locust bean gum (LBG) formulation of microparticles associating both isoniazid and rifabutin	575:710	This work proposes inhalable therapy of pulmonary tuberculosis mediated by a single locust bean gum (LBG) formulation of microparticles associating both isoniazid and rifabutin, complying with requisites of the World Health Organisation of combined therapy.
28669623	4	5	theme	single	612:617	arg1	formulation					641:651	a single locust bean gum (LBG) formulation	610:651	a single locust bean gum (LBG) formulation of microparticles associating both isoniazid and rifabutin	610:710	This work proposes inhalable therapy of pulmonary tuberculosis mediated by a single locust bean gum (LBG) formulation of microparticles associating both isoniazid and rifabutin, complying with requisites of the World Health Organisation of combined therapy.
28669623	4	6	theme	therapy	784:790	arg1	Organisation					759:770	the World Health Organisation	742:770	the World Health Organisation of combined therapy	742:790	This work proposes inhalable therapy of pulmonary tuberculosis mediated by a single locust bean gum (LBG) formulation of microparticles associating both isoniazid and rifabutin, complying with requisites of the World Health Organisation of combined therapy.
28669623	4	7	theme	gum	631:633	arg1	formulation					641:651	a single locust bean gum (LBG) formulation	610:651	a single locust bean gum (LBG) formulation of microparticles associating both isoniazid and rifabutin	610:710	This work proposes inhalable therapy of pulmonary tuberculosis mediated by a single locust bean gum (LBG) formulation of microparticles associating both isoniazid and rifabutin, complying with requisites of the World Health Organisation of combined therapy.
28669623	3	8	theme	pathogenic	383:392	arg1	bacteria					394:401	pathogenic bacteria	383:401	pathogenic bacteria	383:401	As pathogenic bacteria are hosted by alveolar macrophages, the co-localisation of antitubercular drugs and pathogens is thus potentiated by this strategy.
28669623	7	9	theme	moderate	1149:1156	arg1	toxicity					1158:1165	moderate toxicity	1149:1165	moderate toxicity (cell viability around 60%)	1149:1193	Cytotoxicity results indicate moderate toxicity (cell viability around 60%), with a concentration-dependent effect.
28669623	8	10	theme	galactose	1388:1396	arg1	units					1398:1402	mannose and galactose units	1376:1402	mannose and galactose units that are specifically recognised by macrophage surface receptors	1376:1467	Additionally, rat alveolar macrophages evidenced preferential capture of LBG microparticles, possibly due to chemical composition comprising mannose and galactose units that are specifically recognised by macrophage surface receptors.
28669623	4	11	theme	Health	752:757	arg1	Organisation					759:770	the World Health Organisation	742:770	the World Health Organisation of combined therapy	742:790	This work proposes inhalable therapy of pulmonary tuberculosis mediated by a single locust bean gum (LBG) formulation of microparticles associating both isoniazid and rifabutin, complying with requisites of the World Health Organisation of combined therapy.
28669623	4	12	theme	World	746:750	arg1	Organisation					759:770	the World Health Organisation	742:770	the World Health Organisation of combined therapy	742:790	This work proposes inhalable therapy of pulmonary tuberculosis mediated by a single locust bean gum (LBG) formulation of microparticles associating both isoniazid and rifabutin, complying with requisites of the World Health Organisation of combined therapy.
28669623	7	13	theme	Cytotoxicity	1119:1130	arg1	results					1132:1138	Cytotoxicity results	1119:1138	Cytotoxicity results	1119:1138	Cytotoxicity results indicate moderate toxicity (cell viability around 60%), with a concentration-dependent effect.
28669623	3	14	theme	antitubercular	462:475	arg1	drugs					477:481	antitubercular drugs	462:481	antitubercular drugs	462:481	As pathogenic bacteria are hosted by alveolar macrophages, the co-localisation of antitubercular drugs and pathogens is thus potentiated by this strategy.
28669623	4	15	theme	bean	626:629	arg1	formulation					641:651	a single locust bean gum (LBG) formulation	610:651	a single locust bean gum (LBG) formulation of microparticles associating both isoniazid and rifabutin	610:710	This work proposes inhalable therapy of pulmonary tuberculosis mediated by a single locust bean gum (LBG) formulation of microparticles associating both isoniazid and rifabutin, complying with requisites of the World Health Organisation of combined therapy.
28669623	7	16	theme	cell	1168:1171	arg1	viability					1173:1181	cell viability	1168:1181	cell viability around 60%	1168:1192	Cytotoxicity results indicate moderate toxicity (cell viability around 60%), with a concentration-dependent effect.
28669623	8	17	theme	microparticles	1312:1325	arg1	capture					1297:1303	preferential capture	1284:1303	preferential capture of LBG microparticles	1284:1325	Additionally, rat alveolar macrophages evidenced preferential capture of LBG microparticles, possibly due to chemical composition comprising mannose and galactose units that are specifically recognised by macrophage surface receptors.
28669623	3	18	theme	drugs	477:481	arg1	co-localisation					443:457	the co-localisation	439:457	the co-localisation of antitubercular drugs and pathogens	439:495	As pathogenic bacteria are hosted by alveolar macrophages, the co-localisation of antitubercular drugs and pathogens is thus potentiated by this strategy.
28669623	5	19	theme	mass	854:857	arg1	ratio					859:863	LBG/INH/RFB mass ratio	842:863	LBG/INH/RFB mass ratio of 10/1/0.5	842:875	Microparticles were produced by spray-drying, at LBG/INH/RFB mass ratio of 10/1/0.5.
28669623	6	20	dep	reach	1050:1054	arg1	region					1111:1116	the alveolar region	1098:1116	the alveolar region	1098:1116	The aerodynamic characterisation of microparticles revealed emitted doses of more than 90% and fine particle fraction of 38%, thus indicating the adequacy of the system to reach the respiratory lung area, thus partially the alveolar region.
28669623	4	21	theme	combined	775:782	arg1	therapy					784:790	combined therapy	775:790	combined therapy	775:790	This work proposes inhalable therapy of pulmonary tuberculosis mediated by a single locust bean gum (LBG) formulation of microparticles associating both isoniazid and rifabutin, complying with requisites of the World Health Organisation of combined therapy.
28669623	0	22	theme	Dual	0:3	arg1	antibiotherapy					5:18	Dual antibiotherapy	0:18	Dual antibiotherapy of tuberculosis	0:34	Dual antibiotherapy of tuberculosis mediated by inhalable locust bean gum microparticles.
28669623	8	23	theme	preferential	1284:1295	arg1	capture					1297:1303	preferential capture	1284:1303	preferential capture of LBG microparticles	1284:1325	Additionally, rat alveolar macrophages evidenced preferential capture of LBG microparticles, possibly due to chemical composition comprising mannose and galactose units that are specifically recognised by macrophage surface receptors.
28669623	0	24	theme	tuberculosis	23:34	arg1	antibiotherapy					5:18	Dual antibiotherapy	0:18	Dual antibiotherapy of tuberculosis	0:34	Dual antibiotherapy of tuberculosis mediated by inhalable locust bean gum microparticles.
28669623	4	25	theme	tuberculosis	585:596	arg1	therapy					564:570	inhalable therapy	554:570	inhalable therapy of pulmonary tuberculosis mediated by a single locust bean gum (LBG) formulation of microparticles associating both isoniazid and rifabutin	554:710	This work proposes inhalable therapy of pulmonary tuberculosis mediated by a single locust bean gum (LBG) formulation of microparticles associating both isoniazid and rifabutin, complying with requisites of the World Health Organisation of combined therapy.
28669623	1	26	with	resistance	208:217	arg1	treatments					241:250	treatments	241:250	treatments	241:250	Despite the existence of effective oral therapy, tuberculosis remains a deadly pathology, namely because of bacterial resistance and incompliance with treatments.
28669623	8	27	theme	mannose	1376:1382	arg1	units					1398:1402	mannose and galactose units	1376:1402	mannose and galactose units that are specifically recognised by macrophage surface receptors	1376:1467	Additionally, rat alveolar macrophages evidenced preferential capture of LBG microparticles, possibly due to chemical composition comprising mannose and galactose units that are specifically recognised by macrophage surface receptors.
28669623	4	28	theme	microparticles	656:669	arg1	formulation					641:651	a single locust bean gum (LBG) formulation	610:651	a single locust bean gum (LBG) formulation of microparticles associating both isoniazid and rifabutin	610:710	This work proposes inhalable therapy of pulmonary tuberculosis mediated by a single locust bean gum (LBG) formulation of microparticles associating both isoniazid and rifabutin, complying with requisites of the World Health Organisation of combined therapy.
28669623	5	29	theme	10/1/0.5	868:875	arg1	ratio					859:863	LBG/INH/RFB mass ratio	842:863	LBG/INH/RFB mass ratio of 10/1/0.5	842:875	Microparticles were produced by spray-drying, at LBG/INH/RFB mass ratio of 10/1/0.5.
28669623	6	30	theme	emitted	938:944	arg1	doses					946:950	emitted doses	938:950	emitted doses of more than 90%	938:967	The aerodynamic characterisation of microparticles revealed emitted doses of more than 90% and fine particle fraction of 38%, thus indicating the adequacy of the system to reach the respiratory lung area, thus partially the alveolar region.
28669623	2	31	theme	inhalable	324:332	arg1	therapy					334:340	inhalable therapy	324:340	inhalable therapy	324:340	Establishing alternative therapeutic approaches is urgently needed and inhalable therapy has a great potential in this regard.
28669623	1	32	theme	bacterial	198:206	arg1	resistance					208:217	bacterial resistance	198:217	bacterial resistance	198:217	Despite the existence of effective oral therapy, tuberculosis remains a deadly pathology, namely because of bacterial resistance and incompliance with treatments.
28669623	6	33	theme	%	1001:1001	arg1	doses					946:950	emitted doses	938:950	emitted doses of more than 90%	938:967	The aerodynamic characterisation of microparticles revealed emitted doses of more than 90% and fine particle fraction of 38%, thus indicating the adequacy of the system to reach the respiratory lung area, thus partially the alveolar region.
28669623	6	33	theme	%	1001:1001	arg1	fraction					987:994	fine particle fraction	973:994	fine particle fraction of 38%	973:1001	The aerodynamic characterisation of microparticles revealed emitted doses of more than 90% and fine particle fraction of 38%, thus indicating the adequacy of the system to reach the respiratory lung area, thus partially the alveolar region.
28669623	8	34	theme	chemical	1344:1351	arg1	composition					1353:1363	chemical composition	1344:1363	chemical composition comprising mannose and galactose units that are specifically recognised by macrophage surface receptors	1344:1467	Additionally, rat alveolar macrophages evidenced preferential capture of LBG microparticles, possibly due to chemical composition comprising mannose and galactose units that are specifically recognised by macrophage surface receptors.
28669623	6	35	theme	lung	1072:1075	arg1	area					1077:1080	the respiratory lung area	1056:1080	the respiratory lung area	1056:1080	The aerodynamic characterisation of microparticles revealed emitted doses of more than 90% and fine particle fraction of 38%, thus indicating the adequacy of the system to reach the respiratory lung area, thus partially the alveolar region.
28669623	6	36	theme	alveolar	1102:1109	arg1	region					1111:1116	the alveolar region	1098:1116	the alveolar region	1098:1116	The aerodynamic characterisation of microparticles revealed emitted doses of more than 90% and fine particle fraction of 38%, thus indicating the adequacy of the system to reach the respiratory lung area, thus partially the alveolar region.
28669623	6	37	theme	microparticles	914:927	arg1	characterisation					894:909	The aerodynamic characterisation	878:909	The aerodynamic characterisation of microparticles	878:927	The aerodynamic characterisation of microparticles revealed emitted doses of more than 90% and fine particle fraction of 38%, thus indicating the adequacy of the system to reach the respiratory lung area, thus partially the alveolar region.
28669623	7	38	dep	toxicity	1158:1165	arg1	viability					1173:1181	cell viability	1168:1181	cell viability around 60%	1168:1192	Cytotoxicity results indicate moderate toxicity (cell viability around 60%), with a concentration-dependent effect.
28669623	3	39	theme	pathogens	487:495	arg1	co-localisation					443:457	the co-localisation	439:457	the co-localisation of antitubercular drugs and pathogens	439:495	As pathogenic bacteria are hosted by alveolar macrophages, the co-localisation of antitubercular drugs and pathogens is thus potentiated by this strategy.
28669623	3	40	theme	alveolar	417:424	arg1	macrophages					426:436	alveolar macrophages	417:436	alveolar macrophages	417:436	As pathogenic bacteria are hosted by alveolar macrophages, the co-localisation of antitubercular drugs and pathogens is thus potentiated by this strategy.
28669623	4	41	theme	inhalable	554:562	arg1	therapy					564:570	inhalable therapy	554:570	inhalable therapy of pulmonary tuberculosis mediated by a single locust bean gum (LBG) formulation of microparticles associating both isoniazid and rifabutin	554:710	This work proposes inhalable therapy of pulmonary tuberculosis mediated by a single locust bean gum (LBG) formulation of microparticles associating both isoniazid and rifabutin, complying with requisites of the World Health Organisation of combined therapy.
28669623	5	42	theme	LBG/INH/RFB	842:852	arg1	ratio					859:863	LBG/INH/RFB mass ratio	842:863	LBG/INH/RFB mass ratio of 10/1/0.5	842:875	Microparticles were produced by spray-drying, at LBG/INH/RFB mass ratio of 10/1/0.5.
28669623	0	43	theme	bean	65:68	arg1	microparticles					74:87	inhalable locust bean gum microparticles	48:87	inhalable locust bean gum microparticles	48:87	Dual antibiotherapy of tuberculosis mediated by inhalable locust bean gum microparticles.
28669623	1	44	theme	effective	115:123	arg1	therapy					130:136	effective oral therapy	115:136	effective oral therapy	115:136	Despite the existence of effective oral therapy, tuberculosis remains a deadly pathology, namely because of bacterial resistance and incompliance with treatments.
28669623	0	45	theme	inhalable	48:56	arg1	microparticles					74:87	inhalable locust bean gum microparticles	48:87	inhalable locust bean gum microparticles	48:87	Dual antibiotherapy of tuberculosis mediated by inhalable locust bean gum microparticles.
28669623	2	46	contain	has	342:344	arg1	therapy					334:340	inhalable therapy	324:340	inhalable therapy	324:340	Establishing alternative therapeutic approaches is urgently needed and inhalable therapy has a great potential in this regard.
28669623	2	46	contain	has	342:344	arg2	potential					354:362	a great potential	346:362	a great potential	346:362	Establishing alternative therapeutic approaches is urgently needed and inhalable therapy has a great potential in this regard.
28669623	7	47	theme	concentration-dependent	1203:1225	arg1	effect					1227:1232	a concentration-dependent effect	1201:1232	a concentration-dependent effect	1201:1232	Cytotoxicity results indicate moderate toxicity (cell viability around 60%), with a concentration-dependent effect.
28669623	1	48	theme	oral	125:128	arg1	therapy					130:136	effective oral therapy	115:136	effective oral therapy	115:136	Despite the existence of effective oral therapy, tuberculosis remains a deadly pathology, namely because of bacterial resistance and incompliance with treatments.
28669623	6	49	theme	particle	978:985	arg1	fraction					987:994	fine particle fraction	973:994	fine particle fraction of 38%	973:1001	The aerodynamic characterisation of microparticles revealed emitted doses of more than 90% and fine particle fraction of 38%, thus indicating the adequacy of the system to reach the respiratory lung area, thus partially the alveolar region.
28669623	6	50	theme	aerodynamic	882:892	arg1	characterisation					894:909	The aerodynamic characterisation	878:909	The aerodynamic characterisation of microparticles	878:927	The aerodynamic characterisation of microparticles revealed emitted doses of more than 90% and fine particle fraction of 38%, thus indicating the adequacy of the system to reach the respiratory lung area, thus partially the alveolar region.
28669623	8	51	theme	surface	1451:1457	arg1	receptors					1459:1467	macrophage surface receptors	1440:1467	macrophage surface receptors	1440:1467	Additionally, rat alveolar macrophages evidenced preferential capture of LBG microparticles, possibly due to chemical composition comprising mannose and galactose units that are specifically recognised by macrophage surface receptors.
28669623	1	52	theme	therapy	130:136	arg1	existence					102:110	the existence	98:110	the existence of effective oral therapy	98:136	Despite the existence of effective oral therapy, tuberculosis remains a deadly pathology, namely because of bacterial resistance and incompliance with treatments.
28669623	8	53	theme	macrophage	1440:1449	arg1	receptors					1459:1467	macrophage surface receptors	1440:1467	macrophage surface receptors	1440:1467	Additionally, rat alveolar macrophages evidenced preferential capture of LBG microparticles, possibly due to chemical composition comprising mannose and galactose units that are specifically recognised by macrophage surface receptors.
28669623	4	54	theme	LBG	636:638	arg1	formulation					641:651	a single locust bean gum (LBG) formulation	610:651	a single locust bean gum (LBG) formulation of microparticles associating both isoniazid and rifabutin	610:710	This work proposes inhalable therapy of pulmonary tuberculosis mediated by a single locust bean gum (LBG) formulation of microparticles associating both isoniazid and rifabutin, complying with requisites of the World Health Organisation of combined therapy.
28669623	2	55	theme	therapeutic	278:288	arg1	approaches					290:299	alternative therapeutic approaches	266:299	alternative therapeutic approaches	266:299	Establishing alternative therapeutic approaches is urgently needed and inhalable therapy has a great potential in this regard.
28669623	2	56	theme	great	348:352	arg1	potential					354:362	a great potential	346:362	a great potential	346:362	Establishing alternative therapeutic approaches is urgently needed and inhalable therapy has a great potential in this regard.
28669623	8	57	theme	alveolar	1253:1260	arg1	macrophages					1262:1272	rat alveolar macrophages	1249:1272	rat alveolar macrophages	1249:1272	Additionally, rat alveolar macrophages evidenced preferential capture of LBG microparticles, possibly due to chemical composition comprising mannose and galactose units that are specifically recognised by macrophage surface receptors.
28669623	4	58	theme	Organisation	759:770	arg1	requisites					728:737	requisites	728:737	requisites of the World Health Organisation of combined therapy	728:790	This work proposes inhalable therapy of pulmonary tuberculosis mediated by a single locust bean gum (LBG) formulation of microparticles associating both isoniazid and rifabutin, complying with requisites of the World Health Organisation of combined therapy.
28669623	4	58	theme	Organisation	759:770	arg1	Organisation					759:770	the World Health Organisation	742:770	the World Health Organisation of combined therapy	742:790	This work proposes inhalable therapy of pulmonary tuberculosis mediated by a single locust bean gum (LBG) formulation of microparticles associating both isoniazid and rifabutin, complying with requisites of the World Health Organisation of combined therapy.
28669623	0	59	theme	gum	70:72	arg1	microparticles					74:87	inhalable locust bean gum microparticles	48:87	inhalable locust bean gum microparticles	48:87	Dual antibiotherapy of tuberculosis mediated by inhalable locust bean gum microparticles.
28669623	6	60	theme	respiratory	1060:1070	arg1	area					1077:1080	the respiratory lung area	1056:1080	the respiratory lung area	1056:1080	The aerodynamic characterisation of microparticles revealed emitted doses of more than 90% and fine particle fraction of 38%, thus indicating the adequacy of the system to reach the respiratory lung area, thus partially the alveolar region.
28669623	2	61	theme	alternative	266:276	arg1	approaches					290:299	alternative therapeutic approaches	266:299	alternative therapeutic approaches	266:299	Establishing alternative therapeutic approaches is urgently needed and inhalable therapy has a great potential in this regard.
28669623	8	62	theme	LBG	1308:1310	arg1	microparticles					1312:1325	LBG microparticles	1308:1325	LBG microparticles	1308:1325	Additionally, rat alveolar macrophages evidenced preferential capture of LBG microparticles, possibly due to chemical composition comprising mannose and galactose units that are specifically recognised by macrophage surface receptors.
28669623	0	63	theme	locust	58:63	arg1	microparticles					74:87	inhalable locust bean gum microparticles	48:87	inhalable locust bean gum microparticles	48:87	Dual antibiotherapy of tuberculosis mediated by inhalable locust bean gum microparticles.
28669623	6	64	theme	fine	973:976	arg1	fraction					987:994	fine particle fraction	973:994	fine particle fraction of 38%	973:1001	The aerodynamic characterisation of microparticles revealed emitted doses of more than 90% and fine particle fraction of 38%, thus indicating the adequacy of the system to reach the respiratory lung area, thus partially the alveolar region.
28669623	1	65	with	incompliance	223:234	arg1	treatments					241:250	treatments	241:250	treatments	241:250	Despite the existence of effective oral therapy, tuberculosis remains a deadly pathology, namely because of bacterial resistance and incompliance with treatments.
28669623	6	66	theme	system	1040:1045	arg1	adequacy					1024:1031	the adequacy	1020:1031	the adequacy of the system to reach the respiratory lung area, thus partially the alveolar region	1020:1116	The aerodynamic characterisation of microparticles revealed emitted doses of more than 90% and fine particle fraction of 38%, thus indicating the adequacy of the system to reach the respiratory lung area, thus partially the alveolar region.
25817678	1	0	theme	polysaccharides	157:171	arg1	substrates					173:182	different polysaccharides substrates	147:182	different polysaccharides substrates (cellulose powder (CP), microcrystalline cellulose (MCC), carboxymethyl cellulose (CMC) and chitosan (Chit))	147:291	A method to impregnate silver nanoparticles (AgNPs) into different polysaccharides substrates (cellulose powder (CP), microcrystalline cellulose (MCC), carboxymethyl cellulose (CMC) and chitosan (Chit)) by using glucose as reducing agent, is presented.
25817678	2	1	theme	sizes	447:451	arg1	formation					418:426	the formation	414:426	the formation of silver particle sizes	414:451	X-ray diffraction analyses of polysaccharides coated with AgNPs showed the formation of silver particle sizes in the range of 3.7-5.6 nm and have almost spherical shape.
25817678	1	2	theme	carboxymethyl	242:254	arg1	CMC					267:269	CMC	267:269	CMC	267:269	A method to impregnate silver nanoparticles (AgNPs) into different polysaccharides substrates (cellulose powder (CP), microcrystalline cellulose (MCC), carboxymethyl cellulose (CMC) and chitosan (Chit)) by using glucose as reducing agent, is presented.
25817678	1	2	theme	carboxymethyl	242:254	arg1	cellulose					256:264	carboxymethyl cellulose	242:264	carboxymethyl cellulose (CMC)	242:270	A method to impregnate silver nanoparticles (AgNPs) into different polysaccharides substrates (cellulose powder (CP), microcrystalline cellulose (MCC), carboxymethyl cellulose (CMC) and chitosan (Chit)) by using glucose as reducing agent, is presented.
25817678	4	3	dep	coli	698:701	arg1	coli					707:710	E. coli	704:710	E. coli	704:710	The antibacterial activity of polysaccharides loaded with silver nanoparticles was evaluated against Gram-negative Escherichia coli (E. coli) and Gram-positive Staphylococcus aureus (S. aureus) bacteria.
25817678	2	4	theme	nm	477:478	arg1	range					460:464	the range	456:464	the range of 3.7-5.6 nm	456:478	X-ray diffraction analyses of polysaccharides coated with AgNPs showed the formation of silver particle sizes in the range of 3.7-5.6 nm and have almost spherical shape.
25817678	1	5	theme	reducing	313:320	arg1	agent					322:326	reducing agent	313:326	reducing agent	313:326	A method to impregnate silver nanoparticles (AgNPs) into different polysaccharides substrates (cellulose powder (CP), microcrystalline cellulose (MCC), carboxymethyl cellulose (CMC) and chitosan (Chit)) by using glucose as reducing agent, is presented.
25817678	1	6	dep	method	92:97	arg1	impregnate					102:111	impregnate	102:111	to impregnate silver nanoparticles (AgNPs) into different polysaccharides substrates (cellulose powder (CP), microcrystalline cellulose (MCC), carboxymethyl cellulose (CMC) and chitosan (Chit)) by using glucose as reducing agent	99:326	A method to impregnate silver nanoparticles (AgNPs) into different polysaccharides substrates (cellulose powder (CP), microcrystalline cellulose (MCC), carboxymethyl cellulose (CMC) and chitosan (Chit)) by using glucose as reducing agent, is presented.
25817678	2	7	contain	have	484:487	arg2	shape					506:510	almost spherical shape	489:510	almost spherical shape	489:510	X-ray diffraction analyses of polysaccharides coated with AgNPs showed the formation of silver particle sizes in the range of 3.7-5.6 nm and have almost spherical shape.
25817678	2	7	contain	have	484:487	arg1	analyses					361:368	X-ray diffraction analyses	343:368	X-ray diffraction analyses of polysaccharides coated with AgNPs	343:405	X-ray diffraction analyses of polysaccharides coated with AgNPs showed the formation of silver particle sizes in the range of 3.7-5.6 nm and have almost spherical shape.
25817678	4	8	theme	Gram-positive	717:729	arg1	bacteria					765:772	Gram-positive Staphylococcus aureus (S. aureus) bacteria	717:772	Gram-positive Staphylococcus aureus (S. aureus) bacteria	717:772	The antibacterial activity of polysaccharides loaded with silver nanoparticles was evaluated against Gram-negative Escherichia coli (E. coli) and Gram-positive Staphylococcus aureus (S. aureus) bacteria.
25817678	2	9	theme	diffraction	349:359	arg1	analyses					361:368	X-ray diffraction analyses	343:368	X-ray diffraction analyses of polysaccharides coated with AgNPs	343:405	X-ray diffraction analyses of polysaccharides coated with AgNPs showed the formation of silver particle sizes in the range of 3.7-5.6 nm and have almost spherical shape.
25817678	0	10	theme	polysaccharide	42:55	arg1	substrates					57:66	polysaccharide substrates	42:66	polysaccharide substrates	42:66	Impregnation of silver nanoparticles into polysaccharide substrates and their properties.
25817678	1	11	theme	cellulose	185:193	arg1	powder					195:200	cellulose powder	185:200	cellulose powder (CP)	185:205	A method to impregnate silver nanoparticles (AgNPs) into different polysaccharides substrates (cellulose powder (CP), microcrystalline cellulose (MCC), carboxymethyl cellulose (CMC) and chitosan (Chit)) by using glucose as reducing agent, is presented.
25817678	1	11	theme	cellulose	185:193	arg1	CP					203:204	CP	203:204	CP	203:204	A method to impregnate silver nanoparticles (AgNPs) into different polysaccharides substrates (cellulose powder (CP), microcrystalline cellulose (MCC), carboxymethyl cellulose (CMC) and chitosan (Chit)) by using glucose as reducing agent, is presented.
25817678	4	12	theme	silver	629:634	arg1	nanoparticles					636:648	silver nanoparticles	629:648	silver nanoparticles	629:648	The antibacterial activity of polysaccharides loaded with silver nanoparticles was evaluated against Gram-negative Escherichia coli (E. coli) and Gram-positive Staphylococcus aureus (S. aureus) bacteria.
25817678	3	13	theme	entire	517:522	arg1	composite					533:541	The entire prepared composite	513:541	The entire prepared composite	513:541	The entire prepared composite shows antimicrobial effect.
25817678	5	14	theme	excellent	795:803	arg1	activity					819:826	excellent antibacterial activity	795:826	excellent antibacterial activity	795:826	The results suggest excellent antibacterial activity.
25817678	1	15	dep	substrates	173:182	arg1	powder					195:200	cellulose powder	185:200	cellulose powder (CP)	185:205	A method to impregnate silver nanoparticles (AgNPs) into different polysaccharides substrates (cellulose powder (CP), microcrystalline cellulose (MCC), carboxymethyl cellulose (CMC) and chitosan (Chit)) by using glucose as reducing agent, is presented.
25817678	1	15	dep	substrates	173:182	arg1	CP					203:204	CP	203:204	CP	203:204	A method to impregnate silver nanoparticles (AgNPs) into different polysaccharides substrates (cellulose powder (CP), microcrystalline cellulose (MCC), carboxymethyl cellulose (CMC) and chitosan (Chit)) by using glucose as reducing agent, is presented.
25817678	5	16	theme	antibacterial	805:817	arg1	activity					819:826	excellent antibacterial activity	795:826	excellent antibacterial activity	795:826	The results suggest excellent antibacterial activity.
25817678	4	17	theme	aureus	746:751	arg1	bacteria					765:772	Gram-positive Staphylococcus aureus (S. aureus) bacteria	717:772	Gram-positive Staphylococcus aureus (S. aureus) bacteria	717:772	The antibacterial activity of polysaccharides loaded with silver nanoparticles was evaluated against Gram-negative Escherichia coli (E. coli) and Gram-positive Staphylococcus aureus (S. aureus) bacteria.
25817678	3	18	theme	antimicrobial	549:561	arg1	effect					563:568	antimicrobial effect	549:568	antimicrobial effect	549:568	The entire prepared composite shows antimicrobial effect.
25817678	4	19	theme	polysaccharides	601:615	arg1	activity					589:596	The antibacterial activity	571:596	The antibacterial activity of polysaccharides loaded with silver nanoparticles	571:648	The antibacterial activity of polysaccharides loaded with silver nanoparticles was evaluated against Gram-negative Escherichia coli (E. coli) and Gram-positive Staphylococcus aureus (S. aureus) bacteria.
25817678	0	20	theme	silver	16:21	arg1	Impregnation					0:11	Impregnation	0:11	Impregnation of silver	0:21	Impregnation of silver nanoparticles into polysaccharide substrates and their properties.
25817678	2	21	theme	spherical	496:504	arg1	shape					506:510	almost spherical shape	489:510	almost spherical shape	489:510	X-ray diffraction analyses of polysaccharides coated with AgNPs showed the formation of silver particle sizes in the range of 3.7-5.6 nm and have almost spherical shape.
25817678	1	22	theme	silver	113:118	arg1	AgNPs					135:139	AgNPs	135:139	AgNPs	135:139	A method to impregnate silver nanoparticles (AgNPs) into different polysaccharides substrates (cellulose powder (CP), microcrystalline cellulose (MCC), carboxymethyl cellulose (CMC) and chitosan (Chit)) by using glucose as reducing agent, is presented.
25817678	1	22	theme	silver	113:118	arg1	nanoparticles					120:132	silver nanoparticles	113:132	silver nanoparticles (AgNPs) into different polysaccharides substrates (cellulose powder (CP), microcrystalline cellulose (MCC), carboxymethyl cellulose (CMC) and chitosan (Chit))	113:291	A method to impregnate silver nanoparticles (AgNPs) into different polysaccharides substrates (cellulose powder (CP), microcrystalline cellulose (MCC), carboxymethyl cellulose (CMC) and chitosan (Chit)) by using glucose as reducing agent, is presented.
25817678	2	23	theme	polysaccharides	373:387	arg1	analyses					361:368	X-ray diffraction analyses	343:368	X-ray diffraction analyses of polysaccharides coated with AgNPs	343:405	X-ray diffraction analyses of polysaccharides coated with AgNPs showed the formation of silver particle sizes in the range of 3.7-5.6 nm and have almost spherical shape.
25817678	2	24	theme	particle	438:445	arg1	sizes					447:451	silver particle sizes	431:451	silver particle sizes	431:451	X-ray diffraction analyses of polysaccharides coated with AgNPs showed the formation of silver particle sizes in the range of 3.7-5.6 nm and have almost spherical shape.
25817678	1	25	dep	powder	195:200	arg1	Chit					286:289	Chit	286:289	Chit	286:289	A method to impregnate silver nanoparticles (AgNPs) into different polysaccharides substrates (cellulose powder (CP), microcrystalline cellulose (MCC), carboxymethyl cellulose (CMC) and chitosan (Chit)) by using glucose as reducing agent, is presented.
25817678	1	25	dep	powder	195:200	arg1	cellulose					225:233	microcrystalline cellulose	208:233	microcrystalline cellulose (MCC)	208:239	A method to impregnate silver nanoparticles (AgNPs) into different polysaccharides substrates (cellulose powder (CP), microcrystalline cellulose (MCC), carboxymethyl cellulose (CMC) and chitosan (Chit)) by using glucose as reducing agent, is presented.
25817678	1	25	dep	powder	195:200	arg1	MCC					236:238	MCC	236:238	MCC	236:238	A method to impregnate silver nanoparticles (AgNPs) into different polysaccharides substrates (cellulose powder (CP), microcrystalline cellulose (MCC), carboxymethyl cellulose (CMC) and chitosan (Chit)) by using glucose as reducing agent, is presented.
25817678	1	25	dep	powder	195:200	arg1	CMC					267:269	CMC	267:269	CMC	267:269	A method to impregnate silver nanoparticles (AgNPs) into different polysaccharides substrates (cellulose powder (CP), microcrystalline cellulose (MCC), carboxymethyl cellulose (CMC) and chitosan (Chit)) by using glucose as reducing agent, is presented.
25817678	1	25	dep	powder	195:200	arg1	chitosan					276:283	chitosan	276:283	chitosan (Chit)	276:290	A method to impregnate silver nanoparticles (AgNPs) into different polysaccharides substrates (cellulose powder (CP), microcrystalline cellulose (MCC), carboxymethyl cellulose (CMC) and chitosan (Chit)) by using glucose as reducing agent, is presented.
25817678	1	25	dep	powder	195:200	arg1	cellulose					256:264	carboxymethyl cellulose	242:264	carboxymethyl cellulose (CMC)	242:270	A method to impregnate silver nanoparticles (AgNPs) into different polysaccharides substrates (cellulose powder (CP), microcrystalline cellulose (MCC), carboxymethyl cellulose (CMC) and chitosan (Chit)) by using glucose as reducing agent, is presented.
25817678	3	26	theme	prepared	524:531	arg1	composite					533:541	The entire prepared composite	513:541	The entire prepared composite	513:541	The entire prepared composite shows antimicrobial effect.
25817678	2	27	theme	silver	431:436	arg1	sizes					447:451	silver particle sizes	431:451	silver particle sizes	431:451	X-ray diffraction analyses of polysaccharides coated with AgNPs showed the formation of silver particle sizes in the range of 3.7-5.6 nm and have almost spherical shape.
25817678	1	28	theme	microcrystalline	208:223	arg1	MCC					236:238	MCC	236:238	MCC	236:238	A method to impregnate silver nanoparticles (AgNPs) into different polysaccharides substrates (cellulose powder (CP), microcrystalline cellulose (MCC), carboxymethyl cellulose (CMC) and chitosan (Chit)) by using glucose as reducing agent, is presented.
25817678	1	28	theme	microcrystalline	208:223	arg1	cellulose					225:233	microcrystalline cellulose	208:233	microcrystalline cellulose (MCC)	208:239	A method to impregnate silver nanoparticles (AgNPs) into different polysaccharides substrates (cellulose powder (CP), microcrystalline cellulose (MCC), carboxymethyl cellulose (CMC) and chitosan (Chit)) by using glucose as reducing agent, is presented.
25817678	4	29	dep	aureus	746:751	arg1	aureus					757:762	S. aureus	754:762	S. aureus	754:762	The antibacterial activity of polysaccharides loaded with silver nanoparticles was evaluated against Gram-negative Escherichia coli (E. coli) and Gram-positive Staphylococcus aureus (S. aureus) bacteria.
25817678	2	30	theme	X-ray	343:347	arg1	diffraction					349:359	X-ray diffraction	343:359	X-ray diffraction analyses of polysaccharides coated with AgNPs	343:405	X-ray diffraction analyses of polysaccharides coated with AgNPs showed the formation of silver particle sizes in the range of 3.7-5.6 nm and have almost spherical shape.
25817678	1	31	theme	different	147:155	arg1	substrates					173:182	different polysaccharides substrates	147:182	different polysaccharides substrates (cellulose powder (CP), microcrystalline cellulose (MCC), carboxymethyl cellulose (CMC) and chitosan (Chit))	147:291	A method to impregnate silver nanoparticles (AgNPs) into different polysaccharides substrates (cellulose powder (CP), microcrystalline cellulose (MCC), carboxymethyl cellulose (CMC) and chitosan (Chit)) by using glucose as reducing agent, is presented.
25817678	4	32	theme	antibacterial	575:587	arg1	activity					589:596	The antibacterial activity	571:596	The antibacterial activity of polysaccharides loaded with silver nanoparticles	571:648	The antibacterial activity of polysaccharides loaded with silver nanoparticles was evaluated against Gram-negative Escherichia coli (E. coli) and Gram-positive Staphylococcus aureus (S. aureus) bacteria.
28056526	4	0	theme	supply	511:516	arg1	chain					518:522	the supply chain	507:522	the supply chain for this critical drug quite fragile	507:559	The worldwide dependence on a single animal species has made the supply chain for this critical drug quite fragile, leading to the search for other sources of these drugs, including bovine tissues such as bovine intestine or lung.
28056526	1	1	theme	used	166:169	arg1	anticoagulants					180:193	widely used clinical anticoagulants	159:193	widely used clinical anticoagulants	159:193	Heparin and its low-molecular-weight heparin (LMWH) derivatives are widely used clinical anticoagulants.
28056526	1	1	theme	used	166:169	arg1	Heparin					91:97	Heparin	91:97	Heparin	91:97	Heparin and its low-molecular-weight heparin (LMWH) derivatives are widely used clinical anticoagulants.
28056526	1	1	theme	used	166:169	arg1	heparin					128:134	its low-molecular-weight heparin	103:134	its low-molecular-weight heparin (LMWH)	103:141	Heparin and its low-molecular-weight heparin (LMWH) derivatives are widely used clinical anticoagulants.
28056526	5	2	dep	similarities	730:741	arg1	the					726:728	the	726:728	the	726:728	A number of laboratories are currently examining the similarities and differences between heparins prepared from porcine and bovine tissues.
28056526	6	3	theme	current	822:828	arg1	study					830:834	The current study	818:834	The current study	818:834	The current study is designed to compare LMWH prepared from bovine heparins through chemical β-elimination, a process currently used to prepare the LMWH, enoxaparin, from porcine heparin.
28056526	1	4	theme	clinical	171:178	arg1	anticoagulants					180:193	widely used clinical anticoagulants	159:193	widely used clinical anticoagulants	159:193	Heparin and its low-molecular-weight heparin (LMWH) derivatives are widely used clinical anticoagulants.
28056526	1	4	theme	clinical	171:178	arg1	Heparin					91:97	Heparin	91:97	Heparin	91:97	Heparin and its low-molecular-weight heparin (LMWH) derivatives are widely used clinical anticoagulants.
28056526	1	4	theme	clinical	171:178	arg1	heparin					128:134	its low-molecular-weight heparin	103:134	its low-molecular-weight heparin (LMWH)	103:141	Heparin and its low-molecular-weight heparin (LMWH) derivatives are widely used clinical anticoagulants.
28056526	6	5	theme	porcine	989:995	arg1	heparin					997:1003	porcine heparin	989:1003	porcine heparin	989:1003	The current study is designed to compare LMWH prepared from bovine heparins through chemical β-elimination, a process currently used to prepare the LMWH, enoxaparin, from porcine heparin.
28056526	6	6	theme	chemical	902:909	arg1	β-elimination					911:923	chemical β-elimination	902:923	chemical β-elimination	902:923	The current study is designed to compare LMWH prepared from bovine heparins through chemical β-elimination, a process currently used to prepare the LMWH, enoxaparin, from porcine heparin.
28056526	6	6	theme	chemical	902:909	arg1	process					928:934	a process	926:934	a process currently used to prepare the LMWH, enoxaparin, from porcine heparin	926:1003	The current study is designed to compare LMWH prepared from bovine heparins through chemical β-elimination, a process currently used to prepare the LMWH, enoxaparin, from porcine heparin.
28056526	4	7	theme	other	588:592	arg1	tissues					635:641	bovine tissues	628:641	bovine tissues such as bovine intestine or lung	628:674	The worldwide dependence on a single animal species has made the supply chain for this critical drug quite fragile, leading to the search for other sources of these drugs, including bovine tissues such as bovine intestine or lung.
28056526	4	7	theme	other	588:592	arg1	sources					594:600	other sources	588:600	other sources	588:600	The worldwide dependence on a single animal species has made the supply chain for this critical drug quite fragile, leading to the search for other sources of these drugs, including bovine tissues such as bovine intestine or lung.
28056526	2	8	from	practice	229:236	arg1	dialysis					283:290	kidney dialysis	276:290	kidney dialysis	276:290	These drugs are critical for the practice of medicine in applications including kidney dialysis, cardiopulmonary bypass, and in the management of venous thromboembolism.
28056526	2	8	from	practice	229:236	arg1	applications					253:264	applications	253:264	applications including kidney dialysis, cardiopulmonary bypass	253:314	These drugs are critical for the practice of medicine in applications including kidney dialysis, cardiopulmonary bypass, and in the management of venous thromboembolism.
28056526	2	8	from	practice	229:236	arg1	management					328:337	the management	324:337	the management of venous thromboembolism	324:363	These drugs are critical for the practice of medicine in applications including kidney dialysis, cardiopulmonary bypass, and in the management of venous thromboembolism.
28056526	4	9	theme	bovine	651:656	arg1	intestine					658:666	bovine intestine	651:666	bovine intestine	651:666	The worldwide dependence on a single animal species has made the supply chain for this critical drug quite fragile, leading to the search for other sources of these drugs, including bovine tissues such as bovine intestine or lung.
28056526	5	10	theme	bovine	802:807	arg1	tissues					809:815	porcine and bovine tissues	790:815	porcine and bovine tissues	790:815	A number of laboratories are currently examining the similarities and differences between heparins prepared from porcine and bovine tissues.
28056526	7	11	theme	bovine	1091:1096	arg1	lung					1098:1101	bovine lung	1091:1101	bovine lung	1091:1101	Using top-down, bottom-up, compositional analysis and bioassays, LMWHs, derived from bovine lung and intestine, are shown to closely resemble enoxaparin.
28056526	6	12	theme	bovine	878:883	arg1	heparins					885:892	bovine heparins	878:892	bovine heparins	878:892	The current study is designed to compare LMWH prepared from bovine heparins through chemical β-elimination, a process currently used to prepare the LMWH, enoxaparin, from porcine heparin.
28056526	0	13	theme	Heparins	35:42	arg1	Comparison					0:9	Comparison	0:9	Comparison of Low-Molecular-Weight Heparins	0:42	Comparison of Low-Molecular-Weight Heparins Prepared From Bovine Heparins With Enoxaparin.
28056526	1	14	theme	low-molecular-weight	107:126	arg1	anticoagulants					180:193	widely used clinical anticoagulants	159:193	widely used clinical anticoagulants	159:193	Heparin and its low-molecular-weight heparin (LMWH) derivatives are widely used clinical anticoagulants.
28056526	1	14	theme	low-molecular-weight	107:126	arg1	Heparin					91:97	Heparin	91:97	Heparin	91:97	Heparin and its low-molecular-weight heparin (LMWH) derivatives are widely used clinical anticoagulants.
28056526	1	14	theme	low-molecular-weight	107:126	arg1	LMWH					137:140	LMWH	137:140	LMWH	137:140	Heparin and its low-molecular-weight heparin (LMWH) derivatives are widely used clinical anticoagulants.
28056526	1	14	theme	low-molecular-weight	107:126	arg1	heparin					128:134	its low-molecular-weight heparin	103:134	its low-molecular-weight heparin (LMWH)	103:141	Heparin and its low-molecular-weight heparin (LMWH) derivatives are widely used clinical anticoagulants.
28056526	4	15	theme	bovine	628:633	arg1	tissues					635:641	bovine tissues	628:641	bovine tissues such as bovine intestine or lung	628:674	The worldwide dependence on a single animal species has made the supply chain for this critical drug quite fragile, leading to the search for other sources of these drugs, including bovine tissues such as bovine intestine or lung.
28056526	4	15	theme	bovine	628:633	arg1	lung					671:674	lung	671:674	lung	671:674	The worldwide dependence on a single animal species has made the supply chain for this critical drug quite fragile, leading to the search for other sources of these drugs, including bovine tissues such as bovine intestine or lung.
28056526	4	15	theme	bovine	628:633	arg1	intestine					658:666	bovine intestine	651:666	bovine intestine	651:666	The worldwide dependence on a single animal species has made the supply chain for this critical drug quite fragile, leading to the search for other sources of these drugs, including bovine tissues such as bovine intestine or lung.
28056526	0	16	theme	Low-Molecular-Weight	14:33	arg1	Heparins					35:42	Low-Molecular-Weight Heparins	14:42	Low-Molecular-Weight Heparins	14:42	Comparison of Low-Molecular-Weight Heparins Prepared From Bovine Heparins With Enoxaparin.
28056526	6	17	theme	LMWH	966:969	arg1	enoxaparin					972:981	the LMWH, enoxaparin	962:981	enoxaparin	972:981	The current study is designed to compare LMWH prepared from bovine heparins through chemical β-elimination, a process currently used to prepare the LMWH, enoxaparin, from porcine heparin.
28056526	5	18	theme	laboratories	689:700	arg1	number					679:684	A number	677:684	A number of laboratories	677:700	A number of laboratories are currently examining the similarities and differences between heparins prepared from porcine and bovine tissues.
28056526	3	19	theme	porcine	427:433	arg1	intestine					435:443	porcine intestine	427:443	porcine intestine	427:443	Currently, these drugs are derived from livestock, primarily porcine intestine.
28056526	2	20	theme	thromboembolism	349:363	arg1	management					328:337	the management	324:337	the management of venous thromboembolism	324:363	These drugs are critical for the practice of medicine in applications including kidney dialysis, cardiopulmonary bypass, and in the management of venous thromboembolism.
28056526	5	21	theme	porcine	790:796	arg1	tissues					809:815	porcine and bovine tissues	790:815	porcine and bovine tissues	790:815	A number of laboratories are currently examining the similarities and differences between heparins prepared from porcine and bovine tissues.
28056526	4	22	theme	animal	483:488	arg1	species					490:496	a single animal species	474:496	a single animal species	474:496	The worldwide dependence on a single animal species has made the supply chain for this critical drug quite fragile, leading to the search for other sources of these drugs, including bovine tissues such as bovine intestine or lung.
28056526	2	23	theme	medicine	241:248	arg1	practice					229:236	the practice	225:236	the practice of medicine in applications including kidney dialysis, cardiopulmonary bypass, and in the management of venous thromboembolism	225:363	These drugs are critical for the practice of medicine in applications including kidney dialysis, cardiopulmonary bypass, and in the management of venous thromboembolism.
28056526	4	24	theme	critical	533:540	arg1	drug					542:545	this critical drug	528:545	this critical drug quite fragile	528:559	The worldwide dependence on a single animal species has made the supply chain for this critical drug quite fragile, leading to the search for other sources of these drugs, including bovine tissues such as bovine intestine or lung.
28056526	4	25	from	dependence	460:469	arg1	species					490:496	a single animal species	474:496	a single animal species	474:496	The worldwide dependence on a single animal species has made the supply chain for this critical drug quite fragile, leading to the search for other sources of these drugs, including bovine tissues such as bovine intestine or lung.
28056526	4	26	theme	single	476:481	arg1	species					490:496	a single animal species	474:496	a single animal species	474:496	The worldwide dependence on a single animal species has made the supply chain for this critical drug quite fragile, leading to the search for other sources of these drugs, including bovine tissues such as bovine intestine or lung.
28056526	0	27	theme	Bovine	58:63	arg1	Heparins					65:72	Bovine Heparins	58:72	Bovine Heparins With Enoxaparin	58:88	Comparison of Low-Molecular-Weight Heparins Prepared From Bovine Heparins With Enoxaparin.
28056526	4	28	theme	fragile	553:559	arg1	drug					542:545	this critical drug	528:545	this critical drug quite fragile	528:559	The worldwide dependence on a single animal species has made the supply chain for this critical drug quite fragile, leading to the search for other sources of these drugs, including bovine tissues such as bovine intestine or lung.
28056526	1	29	dep	Heparin	91:97	arg1	derivatives					143:153	derivatives	143:153	derivatives	143:153	Heparin and its low-molecular-weight heparin (LMWH) derivatives are widely used clinical anticoagulants.
28056526	2	30	theme	venous	342:347	arg1	thromboembolism					349:363	venous thromboembolism	342:363	venous thromboembolism	342:363	These drugs are critical for the practice of medicine in applications including kidney dialysis, cardiopulmonary bypass, and in the management of venous thromboembolism.
28056526	3	31	attach	derived	393:399	arg2	drugs					383:387	these drugs	377:387	these drugs	377:387	Currently, these drugs are derived from livestock, primarily porcine intestine.
28056526	3	31	attach	derived	393:399	arg1	livestock					406:414	livestock	406:414	livestock	406:414	Currently, these drugs are derived from livestock, primarily porcine intestine.
28056526	2	32	theme	kidney	276:281	arg1	cardiopulmonary					293:307	cardiopulmonary	293:307	cardiopulmonary bypass	293:314	These drugs are critical for the practice of medicine in applications including kidney dialysis, cardiopulmonary bypass, and in the management of venous thromboembolism.
28056526	2	32	theme	kidney	276:281	arg1	dialysis					283:290	kidney dialysis	276:290	kidney dialysis	276:290	These drugs are critical for the practice of medicine in applications including kidney dialysis, cardiopulmonary bypass, and in the management of venous thromboembolism.
28056526	0	33	with	Heparins	65:72	arg1	Enoxaparin					79:88	Enoxaparin	79:88	Enoxaparin	79:88	Comparison of Low-Molecular-Weight Heparins Prepared From Bovine Heparins With Enoxaparin.
28056526	7	34	attach	derived	1078:1084	arg2	LMWHs					1071:1075	LMWHs	1071:1075	LMWHs	1071:1075	Using top-down, bottom-up, compositional analysis and bioassays, LMWHs, derived from bovine lung and intestine, are shown to closely resemble enoxaparin.
28056526	7	34	attach	derived	1078:1084	arg1	intestine					1107:1115	intestine	1107:1115	intestine	1107:1115	Using top-down, bottom-up, compositional analysis and bioassays, LMWHs, derived from bovine lung and intestine, are shown to closely resemble enoxaparin.
28056526	7	34	attach	derived	1078:1084	arg1	lung					1098:1101	bovine lung	1091:1101	bovine lung	1091:1101	Using top-down, bottom-up, compositional analysis and bioassays, LMWHs, derived from bovine lung and intestine, are shown to closely resemble enoxaparin.
28056526	4	35	theme	worldwide	450:458	arg1	dependence					460:469	The worldwide dependence	446:469	The worldwide dependence on a single animal species	446:496	The worldwide dependence on a single animal species has made the supply chain for this critical drug quite fragile, leading to the search for other sources of these drugs, including bovine tissues such as bovine intestine or lung.
28056526	7	36	theme	compositional	1033:1045	arg1	analysis					1047:1054	compositional analysis	1033:1054	compositional analysis	1033:1054	Using top-down, bottom-up, compositional analysis and bioassays, LMWHs, derived from bovine lung and intestine, are shown to closely resemble enoxaparin.
28056526	2	37	dep	cardiopulmonary	293:307	arg1	bypass					309:314	bypass	309:314	bypass	309:314	These drugs are critical for the practice of medicine in applications including kidney dialysis, cardiopulmonary bypass, and in the management of venous thromboembolism.
28056526	4	38	theme	drugs	611:615	arg1	tissues					635:641	bovine tissues	628:641	bovine tissues such as bovine intestine or lung	628:674	The worldwide dependence on a single animal species has made the supply chain for this critical drug quite fragile, leading to the search for other sources of these drugs, including bovine tissues such as bovine intestine or lung.
28056526	4	38	theme	drugs	611:615	arg1	sources					594:600	other sources	588:600	other sources	588:600	The worldwide dependence on a single animal species has made the supply chain for this critical drug quite fragile, leading to the search for other sources of these drugs, including bovine tissues such as bovine intestine or lung.
28147234	10	0	from	value	1417:1421	arg1	MDA-MB-231					1453:1462	MDA-MB-231	1453:1462	MDA-MB-231	1453:1462	The IC50 value of chitosan-alginate-AgNPs on MDA-MB-231 was 4.6mg.
28147234	9	1	theme	cell	1327:1330	arg1	line					1332:1335	The breast cancer cell line	1309:1335	The breast cancer cell line MDA-MB-231	1309:1346	The breast cancer cell line MDA-MB-231 was used to test the anticancer activity of the composites.
28147234	5	2	theme	pore	905:908	arg1	size					910:913	a pore size	903:913	a pore size of 50-500μm	903:925	The dispersion of AgNPs in the porous membrane was uniform with a pore size of 50-500μm.
28147234	2	3	used	used	288:291	arg2	alginate					274:281	alginate	274:281	alginate	274:281	Chitosan and alginate were used owing to their pore-forming capacity, while AgNPs were used for their antimicrobial property.
28147234	2	3	used	used	288:291	arg2	Chitosan					261:268	Chitosan	261:268	Chitosan	261:268	Chitosan and alginate were used owing to their pore-forming capacity, while AgNPs were used for their antimicrobial property.
28147234	4	4	from	presence	802:809	arg1	composites					827:836	the composites	823:836	the composites	823:836	The FT-IR results revealed the presence of a strong chemical interaction between chitosan and alginate due to polyelectrolyte complex; whereas, the XRD results confirmed the presence of AgNPs in the composites.
28147234	3	5	theme	X-ray	554:558	arg1	XRD					573:575	XRD	573:575	XRD	573:575	The developed porous composites of chitosan-alginate-AgNPs were characterized using Fourier transform infrared spectroscopy (FT-IR), ultraviolet-visible spectroscopy, X-ray diffraction (XRD) analysis, and scanning electron microscopy (SEM).
28147234	3	5	theme	X-ray	554:558	arg1	diffraction					560:570	X-ray diffraction	554:570	X-ray diffraction (XRD) analysis	554:585	The developed porous composites of chitosan-alginate-AgNPs were characterized using Fourier transform infrared spectroscopy (FT-IR), ultraviolet-visible spectroscopy, X-ray diffraction (XRD) analysis, and scanning electron microscopy (SEM).
28147234	9	6	used	used	1352:1355	arg2	line					1332:1335	The breast cancer cell line	1309:1335	The breast cancer cell line MDA-MB-231	1309:1346	The breast cancer cell line MDA-MB-231 was used to test the anticancer activity of the composites.
28147234	11	7	theme	huge	1532:1535	arg1	potential					1537:1545	a huge potential	1530:1545	a huge potential for its applications in antimicrobial filtration and cancer treatment	1530:1615	The developed chitosan-alginate-AgNPs composite showed a huge potential for its applications in antimicrobial filtration and cancer treatment.
28147234	11	8	theme	antimicrobial	1571:1583	arg1	filtration					1585:1594	antimicrobial filtration	1571:1594	antimicrobial filtration	1571:1594	The developed chitosan-alginate-AgNPs composite showed a huge potential for its applications in antimicrobial filtration and cancer treatment.
28147234	3	9	theme	electron	601:608	arg1	SEM					622:624	SEM	622:624	SEM	622:624	The developed porous composites of chitosan-alginate-AgNPs were characterized using Fourier transform infrared spectroscopy (FT-IR), ultraviolet-visible spectroscopy, X-ray diffraction (XRD) analysis, and scanning electron microscopy (SEM).
28147234	3	9	theme	electron	601:608	arg1	microscopy					610:619	scanning electron microscopy	592:619	scanning electron microscopy (SEM)	592:625	The developed porous composites of chitosan-alginate-AgNPs were characterized using Fourier transform infrared spectroscopy (FT-IR), ultraviolet-visible spectroscopy, X-ray diffraction (XRD) analysis, and scanning electron microscopy (SEM).
28147234	11	10	theme	cancer	1600:1605	arg1	treatment					1607:1615	cancer treatment	1600:1615	cancer treatment	1600:1615	The developed chitosan-alginate-AgNPs composite showed a huge potential for its applications in antimicrobial filtration and cancer treatment.
28147234	11	11	theme	developed	1479:1487	arg1	composite					1513:1521	The developed chitosan-alginate-AgNPs composite	1475:1521	The developed chitosan-alginate-AgNPs composite	1475:1521	The developed chitosan-alginate-AgNPs composite showed a huge potential for its applications in antimicrobial filtration and cancer treatment.
28147234	8	12	theme	bacterial	1183:1191	arg1	higher					1256:1261	higher	1256:1261	higher	1256:1261	The bacterial filtration efficiency of chitosan-alginate-AgNPs was 1.5-times higher than that of the chitosan-alginate composite.
28147234	8	12	theme	bacterial	1183:1191	arg1	efficiency					1204:1213	The bacterial filtration efficiency	1179:1213	The bacterial filtration efficiency of chitosan-alginate-AgNPs	1179:1240	The bacterial filtration efficiency of chitosan-alginate-AgNPs was 1.5-times higher than that of the chitosan-alginate composite.
28147234	1	13	theme	study	126:130	arg1	aim					114:116	The main aim	105:116	The main aim of this study	105:130	The main aim of this study was to obtain porous antimicrobial composites consisting of chitosan, alginate, and biosynthesized silver nanoparticles (AgNPs).
28147234	7	14	theme	11±1mm	1108:1113	arg1	inhibition					1094:1103	inhibition	1094:1103	inhibition of 11±1mm for the Escherichia coli, and 10±1mm for Staphylococcus aureus	1094:1176	The developed composites resulted in the formation of a zone of inhibition of 11±1mm for the Escherichia coli, and 10±1mm for Staphylococcus aureus.
28147234	5	15	theme	AgNPs	857:861	arg1	dispersion					843:852	The dispersion	839:852	The dispersion of AgNPs in the porous membrane	839:884	The dispersion of AgNPs in the porous membrane was uniform with a pore size of 50-500μm.
28147234	11	16	theme	chitosan-alginate-AgNPs	1489:1511	arg1	composite					1513:1521	The developed chitosan-alginate-AgNPs composite	1475:1521	The developed chitosan-alginate-AgNPs composite	1475:1521	The developed chitosan-alginate-AgNPs composite showed a huge potential for its applications in antimicrobial filtration and cancer treatment.
28147234	4	17	theme	chemical	680:687	arg1	interaction					689:699	a strong chemical interaction	671:699	a strong chemical interaction between chitosan and alginate	671:729	The FT-IR results revealed the presence of a strong chemical interaction between chitosan and alginate due to polyelectrolyte complex; whereas, the XRD results confirmed the presence of AgNPs in the composites.
28147234	0	18	theme	porous	43:48	arg1	Antimicrobial					0:12	Antimicrobial	0:12	Antimicrobial	0:12	Antimicrobial and anticancer activities of porous chitosan-alginate biosynthesized silver nanoparticles.
28147234	8	19	theme	filtration	1193:1202	arg1	higher					1256:1261	higher	1256:1261	higher	1256:1261	The bacterial filtration efficiency of chitosan-alginate-AgNPs was 1.5-times higher than that of the chitosan-alginate composite.
28147234	8	19	theme	filtration	1193:1202	arg1	efficiency					1204:1213	The bacterial filtration efficiency	1179:1213	The bacterial filtration efficiency of chitosan-alginate-AgNPs	1179:1240	The bacterial filtration efficiency of chitosan-alginate-AgNPs was 1.5-times higher than that of the chitosan-alginate composite.
28147234	4	20	theme	strong	673:678	arg1	interaction					689:699	a strong chemical interaction	671:699	a strong chemical interaction between chitosan and alginate	671:729	The FT-IR results revealed the presence of a strong chemical interaction between chitosan and alginate due to polyelectrolyte complex; whereas, the XRD results confirmed the presence of AgNPs in the composites.
28147234	3	21	theme	developed	391:399	arg1	chitosan-alginate-AgNPs					422:444	chitosan-alginate-AgNPs	422:444	chitosan-alginate-AgNPs	422:444	The developed porous composites of chitosan-alginate-AgNPs were characterized using Fourier transform infrared spectroscopy (FT-IR), ultraviolet-visible spectroscopy, X-ray diffraction (XRD) analysis, and scanning electron microscopy (SEM).
28147234	3	21	theme	developed	391:399	arg1	composites					408:417	The developed porous composites	387:417	The developed porous composites of chitosan-alginate-AgNPs	387:444	The developed porous composites of chitosan-alginate-AgNPs were characterized using Fourier transform infrared spectroscopy (FT-IR), ultraviolet-visible spectroscopy, X-ray diffraction (XRD) analysis, and scanning electron microscopy (SEM).
28147234	7	22	theme	inhibition	1094:1103	arg1	zone					1086:1089	a zone	1084:1089	a zone of inhibition of 11±1mm for the Escherichia coli, and 10±1mm for Staphylococcus aureus	1084:1176	The developed composites resulted in the formation of a zone of inhibition of 11±1mm for the Escherichia coli, and 10±1mm for Staphylococcus aureus.
28147234	1	23	theme	main	109:112	arg1	aim					114:116	The main aim	105:116	The main aim of this study	105:130	The main aim of this study was to obtain porous antimicrobial composites consisting of chitosan, alginate, and biosynthesized silver nanoparticles (AgNPs).
28147234	5	24	from	dispersion	843:852	arg1	membrane					877:884	the porous membrane	866:884	the porous membrane	866:884	The dispersion of AgNPs in the porous membrane was uniform with a pore size of 50-500μm.
28147234	8	25	theme	chitosan-alginate	1280:1296	arg1	composite					1298:1306	the chitosan-alginate composite	1276:1306	the chitosan-alginate composite	1276:1306	The bacterial filtration efficiency of chitosan-alginate-AgNPs was 1.5-times higher than that of the chitosan-alginate composite.
28147234	3	26	theme	porous	401:406	arg1	chitosan-alginate-AgNPs					422:444	chitosan-alginate-AgNPs	422:444	chitosan-alginate-AgNPs	422:444	The developed porous composites of chitosan-alginate-AgNPs were characterized using Fourier transform infrared spectroscopy (FT-IR), ultraviolet-visible spectroscopy, X-ray diffraction (XRD) analysis, and scanning electron microscopy (SEM).
28147234	3	26	theme	porous	401:406	arg1	composites					408:417	The developed porous composites	387:417	The developed porous composites of chitosan-alginate-AgNPs	387:444	The developed porous composites of chitosan-alginate-AgNPs were characterized using Fourier transform infrared spectroscopy (FT-IR), ultraviolet-visible spectroscopy, X-ray diffraction (XRD) analysis, and scanning electron microscopy (SEM).
28147234	9	27	dep	line	1332:1335	arg1	MDA-MB-231					1337:1346	MDA-MB-231	1337:1346	The breast cancer cell line MDA-MB-231	1309:1346	The breast cancer cell line MDA-MB-231 was used to test the anticancer activity of the composites.
28147234	4	28	theme	interaction	689:699	arg1	presence					659:666	the presence	655:666	the presence of a strong chemical interaction between chitosan and alginate due to polyelectrolyte complex	655:760	The FT-IR results revealed the presence of a strong chemical interaction between chitosan and alginate due to polyelectrolyte complex; whereas, the XRD results confirmed the presence of AgNPs in the composites.
28147234	8	29	theme	chitosan-alginate-AgNPs	1218:1240	arg1	higher					1256:1261	higher	1256:1261	higher	1256:1261	The bacterial filtration efficiency of chitosan-alginate-AgNPs was 1.5-times higher than that of the chitosan-alginate composite.
28147234	8	29	theme	chitosan-alginate-AgNPs	1218:1240	arg1	efficiency					1204:1213	The bacterial filtration efficiency	1179:1213	The bacterial filtration efficiency of chitosan-alginate-AgNPs	1179:1240	The bacterial filtration efficiency of chitosan-alginate-AgNPs was 1.5-times higher than that of the chitosan-alginate composite.
28147234	9	30	theme	anticancer	1369:1378	arg1	activity					1380:1387	the anticancer activity	1365:1387	the anticancer activity of the composites	1365:1405	The breast cancer cell line MDA-MB-231 was used to test the anticancer activity of the composites.
28147234	7	31	theme	zone	1086:1089	arg1	formation					1071:1079	the formation	1067:1079	the formation of a zone of inhibition of 11±1mm for the Escherichia coli, and 10±1mm for Staphylococcus aureus	1067:1176	The developed composites resulted in the formation of a zone of inhibition of 11±1mm for the Escherichia coli, and 10±1mm for Staphylococcus aureus.
28147234	2	32	used	used	348:351	arg2	AgNPs					337:341	AgNPs	337:341	AgNPs	337:341	Chitosan and alginate were used owing to their pore-forming capacity, while AgNPs were used for their antimicrobial property.
28147234	2	33	theme	pore-forming	308:319	arg1	capacity					321:328	their pore-forming capacity	302:328	their pore-forming capacity	302:328	Chitosan and alginate were used owing to their pore-forming capacity, while AgNPs were used for their antimicrobial property.
28147234	10	34	theme	chitosan-alginate-AgNPs	1426:1448	arg1	value					1417:1421	The IC50 value	1408:1421	The IC50 value of chitosan-alginate-AgNPs on MDA-MB-231	1408:1462	The IC50 value of chitosan-alginate-AgNPs on MDA-MB-231 was 4.6mg.
28147234	10	34	theme	chitosan-alginate-AgNPs	1426:1448	arg1	4.6mg					1468:1472	4.6mg	1468:1472	4.6mg	1468:1472	The IC50 value of chitosan-alginate-AgNPs on MDA-MB-231 was 4.6mg.
28147234	3	35	theme	scanning	592:599	arg1	SEM					622:624	SEM	622:624	SEM	622:624	The developed porous composites of chitosan-alginate-AgNPs were characterized using Fourier transform infrared spectroscopy (FT-IR), ultraviolet-visible spectroscopy, X-ray diffraction (XRD) analysis, and scanning electron microscopy (SEM).
28147234	3	35	theme	scanning	592:599	arg1	microscopy					610:619	scanning electron microscopy	592:619	scanning electron microscopy (SEM)	592:625	The developed porous composites of chitosan-alginate-AgNPs were characterized using Fourier transform infrared spectroscopy (FT-IR), ultraviolet-visible spectroscopy, X-ray diffraction (XRD) analysis, and scanning electron microscopy (SEM).
28147234	1	36	theme	porous	146:151	arg1	composites					167:176	porous antimicrobial composites	146:176	porous antimicrobial composites consisting of chitosan, alginate, and biosynthesized silver nanoparticles (AgNPs)	146:258	The main aim of this study was to obtain porous antimicrobial composites consisting of chitosan, alginate, and biosynthesized silver nanoparticles (AgNPs).
28147234	3	37	theme	chitosan-alginate-AgNPs	422:444	arg1	chitosan-alginate-AgNPs					422:444	chitosan-alginate-AgNPs	422:444	chitosan-alginate-AgNPs	422:444	The developed porous composites of chitosan-alginate-AgNPs were characterized using Fourier transform infrared spectroscopy (FT-IR), ultraviolet-visible spectroscopy, X-ray diffraction (XRD) analysis, and scanning electron microscopy (SEM).
28147234	3	37	theme	chitosan-alginate-AgNPs	422:444	arg1	composites					408:417	The developed porous composites	387:417	The developed porous composites of chitosan-alginate-AgNPs	387:444	The developed porous composites of chitosan-alginate-AgNPs were characterized using Fourier transform infrared spectroscopy (FT-IR), ultraviolet-visible spectroscopy, X-ray diffraction (XRD) analysis, and scanning electron microscopy (SEM).
28147234	4	38	attach	presence	802:809	arg1	composites					827:836	the composites	823:836	the composites	823:836	The FT-IR results revealed the presence of a strong chemical interaction between chitosan and alginate due to polyelectrolyte complex; whereas, the XRD results confirmed the presence of AgNPs in the composites.
28147234	4	38	attach	presence	802:809	arg2	AgNPs					814:818	AgNPs	814:818	AgNPs	814:818	The FT-IR results revealed the presence of a strong chemical interaction between chitosan and alginate due to polyelectrolyte complex; whereas, the XRD results confirmed the presence of AgNPs in the composites.
28147234	1	39	theme	biosynthesized	216:229	arg1	AgNPs					253:257	AgNPs	253:257	AgNPs	253:257	The main aim of this study was to obtain porous antimicrobial composites consisting of chitosan, alginate, and biosynthesized silver nanoparticles (AgNPs).
28147234	1	39	theme	biosynthesized	216:229	arg1	nanoparticles					238:250	biosynthesized silver nanoparticles	216:250	biosynthesized silver nanoparticles (AgNPs)	216:258	The main aim of this study was to obtain porous antimicrobial composites consisting of chitosan, alginate, and biosynthesized silver nanoparticles (AgNPs).
28147234	5	40	theme	porous	870:875	arg1	membrane					877:884	the porous membrane	866:884	the porous membrane	866:884	The dispersion of AgNPs in the porous membrane was uniform with a pore size of 50-500μm.
28147234	6	41	theme	composites	958:967	arg1	activity					942:949	Antimicrobial activity	928:949	Antimicrobial activity of the composites	928:967	Antimicrobial activity of the composites was checked with Escherichia coli and Staphylococcus aureus.
28147234	0	42	dep	chitosan-alginate	50:66	arg1	biosynthesized					68:81	biosynthesized	68:81	chitosan-alginate biosynthesized silver nanoparticles	50:102	Antimicrobial and anticancer activities of porous chitosan-alginate biosynthesized silver nanoparticles.
28147234	7	43	theme	developed	1034:1042	arg1	composites					1044:1053	The developed composites	1030:1053	The developed composites	1030:1053	The developed composites resulted in the formation of a zone of inhibition of 11±1mm for the Escherichia coli, and 10±1mm for Staphylococcus aureus.
28147234	1	44	theme	silver	231:236	arg1	AgNPs					253:257	AgNPs	253:257	AgNPs	253:257	The main aim of this study was to obtain porous antimicrobial composites consisting of chitosan, alginate, and biosynthesized silver nanoparticles (AgNPs).
28147234	1	44	theme	silver	231:236	arg1	nanoparticles					238:250	biosynthesized silver nanoparticles	216:250	biosynthesized silver nanoparticles (AgNPs)	216:258	The main aim of this study was to obtain porous antimicrobial composites consisting of chitosan, alginate, and biosynthesized silver nanoparticles (AgNPs).
28147234	9	45	theme	composites	1396:1405	arg1	activity					1380:1387	the anticancer activity	1365:1387	the anticancer activity of the composites	1365:1405	The breast cancer cell line MDA-MB-231 was used to test the anticancer activity of the composites.
28147234	11	46	from	applications	1555:1566	arg1	filtration					1585:1594	antimicrobial filtration	1571:1594	antimicrobial filtration	1571:1594	The developed chitosan-alginate-AgNPs composite showed a huge potential for its applications in antimicrobial filtration and cancer treatment.
28147234	11	46	from	applications	1555:1566	arg1	treatment					1607:1615	cancer treatment	1600:1615	cancer treatment	1600:1615	The developed chitosan-alginate-AgNPs composite showed a huge potential for its applications in antimicrobial filtration and cancer treatment.
28147234	3	47	dep	Fourier	471:477	arg1	transform					479:487	transform	479:487	transform infrared spectroscopy (FT-IR), ultraviolet-visible spectroscopy, X-ray diffraction (XRD) analysis, and scanning electron microscopy (SEM)	479:625	The developed porous composites of chitosan-alginate-AgNPs were characterized using Fourier transform infrared spectroscopy (FT-IR), ultraviolet-visible spectroscopy, X-ray diffraction (XRD) analysis, and scanning electron microscopy (SEM).
28147234	3	48	theme	ultraviolet-visible	520:538	arg1	spectroscopy					540:551	ultraviolet-visible spectroscopy	520:551	ultraviolet-visible spectroscopy	520:551	The developed porous composites of chitosan-alginate-AgNPs were characterized using Fourier transform infrared spectroscopy (FT-IR), ultraviolet-visible spectroscopy, X-ray diffraction (XRD) analysis, and scanning electron microscopy (SEM).
28147234	7	49	theme	10±1mm	1145:1150	arg1	inhibition					1094:1103	inhibition	1094:1103	inhibition of 11±1mm for the Escherichia coli, and 10±1mm for Staphylococcus aureus	1094:1176	The developed composites resulted in the formation of a zone of inhibition of 11±1mm for the Escherichia coli, and 10±1mm for Staphylococcus aureus.
28147234	9	50	theme	cancer	1320:1325	arg1	line					1332:1335	The breast cancer cell line	1309:1335	The breast cancer cell line MDA-MB-231	1309:1346	The breast cancer cell line MDA-MB-231 was used to test the anticancer activity of the composites.
28147234	4	51	dep	revealed	646:653	arg1	whereas					763:769	whereas	763:769	whereas	763:769	The FT-IR results revealed the presence of a strong chemical interaction between chitosan and alginate due to polyelectrolyte complex; whereas, the XRD results confirmed the presence of AgNPs in the composites.
28147234	3	52	dep	transform	479:487	arg1	infrared					489:496	infrared	489:496	transform infrared spectroscopy (FT-IR), ultraviolet-visible spectroscopy, X-ray diffraction (XRD) analysis, and scanning electron microscopy (SEM)	479:625	The developed porous composites of chitosan-alginate-AgNPs were characterized using Fourier transform infrared spectroscopy (FT-IR), ultraviolet-visible spectroscopy, X-ray diffraction (XRD) analysis, and scanning electron microscopy (SEM).
28147234	5	53	theme	50-500μm	918:925	arg1	size					910:913	a pore size	903:913	a pore size of 50-500μm	903:925	The dispersion of AgNPs in the porous membrane was uniform with a pore size of 50-500μm.
28147234	4	54	theme	polyelectrolyte	738:752	arg1	complex					754:760	polyelectrolyte complex	738:760	polyelectrolyte complex	738:760	The FT-IR results revealed the presence of a strong chemical interaction between chitosan and alginate due to polyelectrolyte complex; whereas, the XRD results confirmed the presence of AgNPs in the composites.
28147234	4	55	theme	AgNPs	814:818	arg1	presence					802:809	the presence	798:809	the presence of AgNPs in the composites	798:836	The FT-IR results revealed the presence of a strong chemical interaction between chitosan and alginate due to polyelectrolyte complex; whereas, the XRD results confirmed the presence of AgNPs in the composites.
28147234	4	56	theme	XRD	776:778	arg1	results					780:786	the XRD results	772:786	the XRD results	772:786	The FT-IR results revealed the presence of a strong chemical interaction between chitosan and alginate due to polyelectrolyte complex; whereas, the XRD results confirmed the presence of AgNPs in the composites.
28147234	4	57	theme	FT-IR	632:636	arg1	results					638:644	The FT-IR results	628:644	The FT-IR results	628:644	The FT-IR results revealed the presence of a strong chemical interaction between chitosan and alginate due to polyelectrolyte complex; whereas, the XRD results confirmed the presence of AgNPs in the composites.
28147234	1	58	theme	antimicrobial	153:165	arg1	composites					167:176	porous antimicrobial composites	146:176	porous antimicrobial composites consisting of chitosan, alginate, and biosynthesized silver nanoparticles (AgNPs)	146:258	The main aim of this study was to obtain porous antimicrobial composites consisting of chitosan, alginate, and biosynthesized silver nanoparticles (AgNPs).
28147234	9	59	theme	breast	1313:1318	arg1	line					1332:1335	The breast cancer cell line	1309:1335	The breast cancer cell line MDA-MB-231	1309:1346	The breast cancer cell line MDA-MB-231 was used to test the anticancer activity of the composites.
28147234	3	60	theme	diffraction	560:570	arg1	analysis					578:585	X-ray diffraction (XRD) analysis	554:585	X-ray diffraction (XRD) analysis	554:585	The developed porous composites of chitosan-alginate-AgNPs were characterized using Fourier transform infrared spectroscopy (FT-IR), ultraviolet-visible spectroscopy, X-ray diffraction (XRD) analysis, and scanning electron microscopy (SEM).
28147234	10	61	theme	IC50	1412:1415	arg1	value					1417:1421	The IC50 value	1408:1421	The IC50 value of chitosan-alginate-AgNPs on MDA-MB-231	1408:1462	The IC50 value of chitosan-alginate-AgNPs on MDA-MB-231 was 4.6mg.
28147234	10	61	theme	IC50	1412:1415	arg1	4.6mg					1468:1472	4.6mg	1468:1472	4.6mg	1468:1472	The IC50 value of chitosan-alginate-AgNPs on MDA-MB-231 was 4.6mg.
28147234	0	62	theme	silver	83:88	arg1	nanoparticles					90:102	silver nanoparticles	83:102	silver nanoparticles	83:102	Antimicrobial and anticancer activities of porous chitosan-alginate biosynthesized silver nanoparticles.
28147234	6	63	theme	Antimicrobial	928:940	arg1	activity					942:949	Antimicrobial activity	928:949	Antimicrobial activity of the composites	928:967	Antimicrobial activity of the composites was checked with Escherichia coli and Staphylococcus aureus.
28147234	0	64	dep	Antimicrobial	0:12	arg1	activities					29:38	activities	29:38	activities	29:38	Antimicrobial and anticancer activities of porous chitosan-alginate biosynthesized silver nanoparticles.
28147234	2	65	theme	antimicrobial	363:375	arg1	property					377:384	their antimicrobial property	357:384	their antimicrobial property	357:384	Chitosan and alginate were used owing to their pore-forming capacity, while AgNPs were used for their antimicrobial property.
28363553	5	0	theme	M2	921:922	arg1	mechanism					908:916	the hemostatic mechanism	893:916	the hemostatic mechanism of M2, which can promote blood absorption and platelet activation and could be directly involved in the intrinsic coagulation pathway to accelerate hemostasis	893:1075	These findings were used to evaluate the hemostatic mechanism of M2, which can promote blood absorption and platelet activation and could be directly involved in the intrinsic coagulation pathway to accelerate hemostasis.
28363553	4	1	theme	thrombin	760:767	arg1	TT					775:776	TT	775:776	TT	775:776	The results illustrated that M2 could reduce the length of the activated partial thromboplastin time (APTT) and thrombin time (TT) and presented rapid hemostatic efficiency in the two injury models (P<0.05).
28363553	4	1	theme	thrombin	760:767	arg1	time					769:772	thrombin time	760:772	thrombin time (TT)	760:777	The results illustrated that M2 could reduce the length of the activated partial thromboplastin time (APTT) and thrombin time (TT) and presented rapid hemostatic efficiency in the two injury models (P<0.05).
28363553	3	2	theme	in	572:573	arg1	time					593:596	the in vitro coagulation time	568:596	the in vitro coagulation time	568:596	M2 presented the best physical properties and platelet activation and was thus selected for the investigations of the in vitro coagulation time and hemostatic and biological effects on animals.
28363553	1	3	theme	single-collagen	272:286	arg1	sponges					288:294	single-collagen sponges	272:294	single-collagen sponges	272:294	Sponges composed of different levels of composite collagen/oxidized microcrystalline cellulose (collagen/OMCC), denoted M1-M4, were studied to improve the hemostatic effect of single-collagen sponges.
28363553	5	4	theme	blood	943:947	arg1	absorption					949:958	blood absorption	943:958	blood absorption	943:958	These findings were used to evaluate the hemostatic mechanism of M2, which can promote blood absorption and platelet activation and could be directly involved in the intrinsic coagulation pathway to accelerate hemostasis.
28363553	1	5	theme	sponges	288:294	arg1	effect					262:267	the hemostatic effect	247:267	the hemostatic effect of single-collagen sponges	247:294	Sponges composed of different levels of composite collagen/oxidized microcrystalline cellulose (collagen/OMCC), denoted M1-M4, were studied to improve the hemostatic effect of single-collagen sponges.
28363553	5	6	theme	intrinsic	1022:1030	arg1	pathway					1044:1050	the intrinsic coagulation pathway	1018:1050	the intrinsic coagulation pathway to accelerate hemostasis	1018:1075	These findings were used to evaluate the hemostatic mechanism of M2, which can promote blood absorption and platelet activation and could be directly involved in the intrinsic coagulation pathway to accelerate hemostasis.
28363553	4	7	theme	rapid	793:797	arg1	efficiency					810:819	rapid hemostatic efficiency	793:819	rapid hemostatic efficiency	793:819	The results illustrated that M2 could reduce the length of the activated partial thromboplastin time (APTT) and thrombin time (TT) and presented rapid hemostatic efficiency in the two injury models (P<0.05).
28363553	3	8	dep	in	572:573	arg1	vitro					575:579	vitro	575:579	vitro	575:579	M2 presented the best physical properties and platelet activation and was thus selected for the investigations of the in vitro coagulation time and hemostatic and biological effects on animals.
28363553	3	9	theme	biological	617:626	arg1	effects					628:634	hemostatic and biological effects	602:634	hemostatic and biological effects on animals	602:645	M2 presented the best physical properties and platelet activation and was thus selected for the investigations of the in vitro coagulation time and hemostatic and biological effects on animals.
28363553	3	10	from	time	593:596	arg1	animals					639:645	animals	639:645	animals	639:645	M2 presented the best physical properties and platelet activation and was thus selected for the investigations of the in vitro coagulation time and hemostatic and biological effects on animals.
28363553	5	11	used	used	876:879	arg2	findings					862:869	These findings	856:869	These findings	856:869	These findings were used to evaluate the hemostatic mechanism of M2, which can promote blood absorption and platelet activation and could be directly involved in the intrinsic coagulation pathway to accelerate hemostasis.
28363553	4	12	theme	thromboplastin	729:742	arg1	APTT					750:753	APTT	750:753	APTT	750:753	The results illustrated that M2 could reduce the length of the activated partial thromboplastin time (APTT) and thrombin time (TT) and presented rapid hemostatic efficiency in the two injury models (P<0.05).
28363553	4	12	theme	thromboplastin	729:742	arg1	time					744:747	the activated partial thromboplastin time	707:747	the activated partial thromboplastin time (APTT)	707:754	The results illustrated that M2 could reduce the length of the activated partial thromboplastin time (APTT) and thrombin time (TT) and presented rapid hemostatic efficiency in the two injury models (P<0.05).
28363553	2	13	theme	morphological	305:317	arg1	observations					319:330	Surface morphological observations	297:330	Surface morphological observations	297:330	Surface morphological observations showed that structural combinations and intermolecular interactions occurred between collagen and OMCC in the composites.
28363553	4	14	theme	partial	721:727	arg1	APTT					750:753	APTT	750:753	APTT	750:753	The results illustrated that M2 could reduce the length of the activated partial thromboplastin time (APTT) and thrombin time (TT) and presented rapid hemostatic efficiency in the two injury models (P<0.05).
28363553	4	14	theme	partial	721:727	arg1	time					744:747	the activated partial thromboplastin time	707:747	the activated partial thromboplastin time (APTT)	707:754	The results illustrated that M2 could reduce the length of the activated partial thromboplastin time (APTT) and thrombin time (TT) and presented rapid hemostatic efficiency in the two injury models (P<0.05).
28363553	3	15	from	investigations	550:563	arg1	animals					639:645	animals	639:645	animals	639:645	M2 presented the best physical properties and platelet activation and was thus selected for the investigations of the in vitro coagulation time and hemostatic and biological effects on animals.
28363553	2	16	theme	intermolecular	372:385	arg1	interactions					387:398	intermolecular interactions	372:398	intermolecular interactions	372:398	Surface morphological observations showed that structural combinations and intermolecular interactions occurred between collagen and OMCC in the composites.
28363553	0	17	theme	Reinforced	0:9	arg1	collagen					11:18	Reinforced collagen	0:18	Reinforced collagen with oxidized microcrystalline cellulose	0:59	Reinforced collagen with oxidized microcrystalline cellulose shows improved hemostatic effects.
28363553	3	18	dep	properties	485:494	arg1	the					467:469	the	467:469	the	467:469	M2 presented the best physical properties and platelet activation and was thus selected for the investigations of the in vitro coagulation time and hemostatic and biological effects on animals.
28363553	5	19	dep	pathway	1044:1050	arg1	accelerate					1055:1064	accelerate	1055:1064	to accelerate hemostasis	1052:1075	These findings were used to evaluate the hemostatic mechanism of M2, which can promote blood absorption and platelet activation and could be directly involved in the intrinsic coagulation pathway to accelerate hemostasis.
28363553	3	20	theme	platelet	500:507	arg1	activation					509:518	platelet activation	500:518	platelet activation	500:518	M2 presented the best physical properties and platelet activation and was thus selected for the investigations of the in vitro coagulation time and hemostatic and biological effects on animals.
28363553	0	21	theme	oxidized	25:32	arg1	cellulose					51:59	oxidized microcrystalline cellulose	25:59	oxidized microcrystalline cellulose	25:59	Reinforced collagen with oxidized microcrystalline cellulose shows improved hemostatic effects.
28363553	1	22	theme	different	116:124	arg1	levels					126:131	different levels	116:131	different levels of composite collagen/oxidized microcrystalline cellulose (collagen/OMCC), denoted M1-M4,	116:221	Sponges composed of different levels of composite collagen/oxidized microcrystalline cellulose (collagen/OMCC), denoted M1-M4, were studied to improve the hemostatic effect of single-collagen sponges.
28363553	2	23	theme	structural	344:353	arg1	combinations					355:366	structural combinations	344:366	structural combinations	344:366	Surface morphological observations showed that structural combinations and intermolecular interactions occurred between collagen and OMCC in the composites.
28363553	1	24	theme	cellulose	181:189	arg1	levels					126:131	different levels	116:131	different levels of composite collagen/oxidized microcrystalline cellulose (collagen/OMCC), denoted M1-M4,	116:221	Sponges composed of different levels of composite collagen/oxidized microcrystalline cellulose (collagen/OMCC), denoted M1-M4, were studied to improve the hemostatic effect of single-collagen sponges.
28363553	3	25	theme	physical	476:483	arg1	properties					485:494	physical properties	476:494	physical properties	476:494	M2 presented the best physical properties and platelet activation and was thus selected for the investigations of the in vitro coagulation time and hemostatic and biological effects on animals.
28363553	4	26	theme	time	769:772	arg1	length					697:702	the length	693:702	the length of the activated partial thromboplastin time (APTT) and thrombin time (TT)	693:777	The results illustrated that M2 could reduce the length of the activated partial thromboplastin time (APTT) and thrombin time (TT) and presented rapid hemostatic efficiency in the two injury models (P<0.05).
28363553	4	27	theme	time	744:747	arg1	length					697:702	the length	693:702	the length of the activated partial thromboplastin time (APTT) and thrombin time (TT)	693:777	The results illustrated that M2 could reduce the length of the activated partial thromboplastin time (APTT) and thrombin time (TT) and presented rapid hemostatic efficiency in the two injury models (P<0.05).
28363553	4	28	theme	activated	711:719	arg1	APTT					750:753	APTT	750:753	APTT	750:753	The results illustrated that M2 could reduce the length of the activated partial thromboplastin time (APTT) and thrombin time (TT) and presented rapid hemostatic efficiency in the two injury models (P<0.05).
28363553	4	28	theme	activated	711:719	arg1	time					744:747	the activated partial thromboplastin time	707:747	the activated partial thromboplastin time (APTT)	707:754	The results illustrated that M2 could reduce the length of the activated partial thromboplastin time (APTT) and thrombin time (TT) and presented rapid hemostatic efficiency in the two injury models (P<0.05).
28363553	0	29	theme	microcrystalline	34:49	arg1	cellulose					51:59	oxidized microcrystalline cellulose	25:59	oxidized microcrystalline cellulose	25:59	Reinforced collagen with oxidized microcrystalline cellulose shows improved hemostatic effects.
28363553	4	30	theme	hemostatic	799:808	arg1	efficiency					810:819	rapid hemostatic efficiency	793:819	rapid hemostatic efficiency	793:819	The results illustrated that M2 could reduce the length of the activated partial thromboplastin time (APTT) and thrombin time (TT) and presented rapid hemostatic efficiency in the two injury models (P<0.05).
28363553	0	31	theme	improved	67:74	arg1	effects					87:93	improved hemostatic effects	67:93	improved hemostatic effects	67:93	Reinforced collagen with oxidized microcrystalline cellulose shows improved hemostatic effects.
28363553	4	32	attach	presented	783:791	arg1	P<0.05					847:852	P<0.05	847:852	P<0.05	847:852	The results illustrated that M2 could reduce the length of the activated partial thromboplastin time (APTT) and thrombin time (TT) and presented rapid hemostatic efficiency in the two injury models (P<0.05).
28363553	4	32	attach	presented	783:791	arg2	M2					677:678	M2	677:678	M2	677:678	The results illustrated that M2 could reduce the length of the activated partial thromboplastin time (APTT) and thrombin time (TT) and presented rapid hemostatic efficiency in the two injury models (P<0.05).
28363553	4	32	attach	presented	783:791	arg1	models					839:844	the two injury models	824:844	the two injury models (P<0.05)	824:853	The results illustrated that M2 could reduce the length of the activated partial thromboplastin time (APTT) and thrombin time (TT) and presented rapid hemostatic efficiency in the two injury models (P<0.05).
28363553	6	33	theme	subcutaneous	1146:1157	arg1	tissue					1159:1164	subcutaneous tissue	1146:1164	subcutaneous tissue	1146:1164	Furthermore, M2 was not cytotoxic and was completely biodegraded in subcutaneous tissue within 28days.
28363553	3	34	theme	best	471:474	arg1	properties					485:494	physical properties	476:494	physical properties	476:494	M2 presented the best physical properties and platelet activation and was thus selected for the investigations of the in vitro coagulation time and hemostatic and biological effects on animals.
28363553	0	35	with	collagen	11:18	arg1	cellulose					51:59	oxidized microcrystalline cellulose	25:59	oxidized microcrystalline cellulose	25:59	Reinforced collagen with oxidized microcrystalline cellulose shows improved hemostatic effects.
28363553	1	36	theme	composite	136:144	arg1	collagen/OMCC					192:204	collagen/OMCC	192:204	collagen/OMCC	192:204	Sponges composed of different levels of composite collagen/oxidized microcrystalline cellulose (collagen/OMCC), denoted M1-M4, were studied to improve the hemostatic effect of single-collagen sponges.
28363553	1	36	theme	composite	136:144	arg1	cellulose					181:189	composite collagen/oxidized microcrystalline cellulose	136:189	composite collagen/oxidized microcrystalline cellulose (collagen/OMCC)	136:205	Sponges composed of different levels of composite collagen/oxidized microcrystalline cellulose (collagen/OMCC), denoted M1-M4, were studied to improve the hemostatic effect of single-collagen sponges.
28363553	3	37	from	effects	628:634	arg1	animals					639:645	animals	639:645	animals	639:645	M2 presented the best physical properties and platelet activation and was thus selected for the investigations of the in vitro coagulation time and hemostatic and biological effects on animals.
28363553	5	38	theme	platelet	964:971	arg1	activation					973:982	platelet activation	964:982	platelet activation	964:982	These findings were used to evaluate the hemostatic mechanism of M2, which can promote blood absorption and platelet activation and could be directly involved in the intrinsic coagulation pathway to accelerate hemostasis.
28363553	2	39	theme	Surface	297:303	arg1	observations					319:330	Surface morphological observations	297:330	Surface morphological observations	297:330	Surface morphological observations showed that structural combinations and intermolecular interactions occurred between collagen and OMCC in the composites.
28363553	1	40	theme	collagen/oxidized	146:162	arg1	collagen/OMCC					192:204	collagen/OMCC	192:204	collagen/OMCC	192:204	Sponges composed of different levels of composite collagen/oxidized microcrystalline cellulose (collagen/OMCC), denoted M1-M4, were studied to improve the hemostatic effect of single-collagen sponges.
28363553	1	40	theme	collagen/oxidized	146:162	arg1	cellulose					181:189	composite collagen/oxidized microcrystalline cellulose	136:189	composite collagen/oxidized microcrystalline cellulose (collagen/OMCC)	136:205	Sponges composed of different levels of composite collagen/oxidized microcrystalline cellulose (collagen/OMCC), denoted M1-M4, were studied to improve the hemostatic effect of single-collagen sponges.
28363553	3	41	theme	effects	628:634	arg1	investigations					550:563	the investigations	546:563	the investigations of the in vitro coagulation time and hemostatic and biological effects on animals	546:645	M2 presented the best physical properties and platelet activation and was thus selected for the investigations of the in vitro coagulation time and hemostatic and biological effects on animals.
28363553	3	42	theme	hemostatic	602:611	arg1	effects					628:634	hemostatic and biological effects	602:634	hemostatic and biological effects on animals	602:645	M2 presented the best physical properties and platelet activation and was thus selected for the investigations of the in vitro coagulation time and hemostatic and biological effects on animals.
28363553	4	43	theme	injury	832:837	arg1	P<0.05					847:852	P<0.05	847:852	P<0.05	847:852	The results illustrated that M2 could reduce the length of the activated partial thromboplastin time (APTT) and thrombin time (TT) and presented rapid hemostatic efficiency in the two injury models (P<0.05).
28363553	4	43	theme	injury	832:837	arg1	models					839:844	the two injury models	824:844	the two injury models (P<0.05)	824:853	The results illustrated that M2 could reduce the length of the activated partial thromboplastin time (APTT) and thrombin time (TT) and presented rapid hemostatic efficiency in the two injury models (P<0.05).
28363553	1	44	theme	microcrystalline	164:179	arg1	collagen/OMCC					192:204	collagen/OMCC	192:204	collagen/OMCC	192:204	Sponges composed of different levels of composite collagen/oxidized microcrystalline cellulose (collagen/OMCC), denoted M1-M4, were studied to improve the hemostatic effect of single-collagen sponges.
28363553	1	44	theme	microcrystalline	164:179	arg1	cellulose					181:189	composite collagen/oxidized microcrystalline cellulose	136:189	composite collagen/oxidized microcrystalline cellulose (collagen/OMCC)	136:205	Sponges composed of different levels of composite collagen/oxidized microcrystalline cellulose (collagen/OMCC), denoted M1-M4, were studied to improve the hemostatic effect of single-collagen sponges.
28363553	3	45	from	animals	639:645	arg1	investigations					550:563	the investigations	546:563	the investigations of the in vitro coagulation time and hemostatic and biological effects on animals	546:645	M2 presented the best physical properties and platelet activation and was thus selected for the investigations of the in vitro coagulation time and hemostatic and biological effects on animals.
28363553	1	46	theme	hemostatic	251:260	arg1	effect					262:267	the hemostatic effect	247:267	the hemostatic effect of single-collagen sponges	247:294	Sponges composed of different levels of composite collagen/oxidized microcrystalline cellulose (collagen/OMCC), denoted M1-M4, were studied to improve the hemostatic effect of single-collagen sponges.
28363553	5	47	theme	coagulation	1032:1042	arg1	pathway					1044:1050	the intrinsic coagulation pathway	1018:1050	the intrinsic coagulation pathway to accelerate hemostasis	1018:1075	These findings were used to evaluate the hemostatic mechanism of M2, which can promote blood absorption and platelet activation and could be directly involved in the intrinsic coagulation pathway to accelerate hemostasis.
28363553	3	48	theme	coagulation	581:591	arg1	time					593:596	the in vitro coagulation time	568:596	the in vitro coagulation time	568:596	M2 presented the best physical properties and platelet activation and was thus selected for the investigations of the in vitro coagulation time and hemostatic and biological effects on animals.
28363553	5	49	theme	hemostatic	897:906	arg1	mechanism					908:916	the hemostatic mechanism	893:916	the hemostatic mechanism of M2, which can promote blood absorption and platelet activation and could be directly involved in the intrinsic coagulation pathway to accelerate hemostasis	893:1075	These findings were used to evaluate the hemostatic mechanism of M2, which can promote blood absorption and platelet activation and could be directly involved in the intrinsic coagulation pathway to accelerate hemostasis.
28363553	0	50	theme	hemostatic	76:85	arg1	effects					87:93	improved hemostatic effects	67:93	improved hemostatic effects	67:93	Reinforced collagen with oxidized microcrystalline cellulose shows improved hemostatic effects.
28363553	3	51	theme	time	593:596	arg1	investigations					550:563	the investigations	546:563	the investigations of the in vitro coagulation time and hemostatic and biological effects on animals	546:645	M2 presented the best physical properties and platelet activation and was thus selected for the investigations of the in vitro coagulation time and hemostatic and biological effects on animals.
27987831	5	0	theme	simulated	768:776	arg1	SBF					790:792	SBF	790:792	SBF	790:792	Fourier transform infrared spectroscopy (FTIR) and SEM images confirmed hydroxyapatite deposition in vitro after incubation in simulated body fluid (SBF).
27987831	5	0	theme	simulated	768:776	arg1	fluid					783:787	simulated body fluid	768:787	simulated body fluid (SBF)	768:793	Fourier transform infrared spectroscopy (FTIR) and SEM images confirmed hydroxyapatite deposition in vitro after incubation in simulated body fluid (SBF).
27987831	4	1	theme	Injectability	476:488	arg1	tests					490:494	Injectability tests	476:494	Injectability tests	476:494	Injectability tests and scanning electron microscopy (SEM) results demonstrated the formation of uniform injectable paste-like composites using BG particles and Cn.
27987831	8	2	theme	radiographic	1024:1035	arg1	images					1037:1042	radiographic images	1024:1042	radiographic images	1024:1042	Manual palpation, radiographic images and pathological assessments proved that the composites promote bone formation.
27987831	7	3	theme	in	892:893	arg1	study					900:904	An in vivo study	889:904	An in vivo study in a rat spinal fusion model	889:933	An in vivo study in a rat spinal fusion model confirmed that increasing the amount of BG improved osteoconductivity.
27987831	5	4	theme	body	778:781	arg1	SBF					790:792	SBF	790:792	SBF	790:792	Fourier transform infrared spectroscopy (FTIR) and SEM images confirmed hydroxyapatite deposition in vitro after incubation in simulated body fluid (SBF).
27987831	5	4	theme	body	778:781	arg1	fluid					783:787	simulated body fluid	768:787	simulated body fluid (SBF)	768:793	Fourier transform infrared spectroscopy (FTIR) and SEM images confirmed hydroxyapatite deposition in vitro after incubation in simulated body fluid (SBF).
27987831	4	5	theme	scanning	500:507	arg1	SEM					530:532	SEM	530:532	SEM	530:532	Injectability tests and scanning electron microscopy (SEM) results demonstrated the formation of uniform injectable paste-like composites using BG particles and Cn.
27987831	4	5	theme	scanning	500:507	arg1	microscopy					518:527	scanning electron microscopy	500:527	scanning electron microscopy (SEM) results	500:541	Injectability tests and scanning electron microscopy (SEM) results demonstrated the formation of uniform injectable paste-like composites using BG particles and Cn.
27987831	9	6	theme	potential	1179:1187	arg1	bone					1268:1271	a minimally invasive bone	1247:1271	a minimally invasive bone substitute	1247:1282	Based on these data, the synthesized composites have a potential application in orthopedic and reconstructive surgeries as a minimally invasive bone substitute.
27987831	9	6	theme	potential	1179:1187	arg1	application					1189:1199	a potential application	1177:1199	a potential application in orthopedic and reconstructive surgeries	1177:1242	Based on these data, the synthesized composites have a potential application in orthopedic and reconstructive surgeries as a minimally invasive bone substitute.
27987831	9	7	from	application	1189:1199	arg1	surgeries					1234:1242	orthopedic and reconstructive surgeries	1204:1242	orthopedic and reconstructive surgeries	1204:1242	Based on these data, the synthesized composites have a potential application in orthopedic and reconstructive surgeries as a minimally invasive bone substitute.
27987831	5	8	theme	infrared	659:666	arg1	FTIR					682:685	FTIR	682:685	FTIR	682:685	Fourier transform infrared spectroscopy (FTIR) and SEM images confirmed hydroxyapatite deposition in vitro after incubation in simulated body fluid (SBF).
27987831	5	8	theme	infrared	659:666	arg1	spectroscopy					668:679	infrared spectroscopy	659:679	infrared spectroscopy (FTIR)	659:686	Fourier transform infrared spectroscopy (FTIR) and SEM images confirmed hydroxyapatite deposition in vitro after incubation in simulated body fluid (SBF).
27987831	6	9	theme	BG	803:804	arg1	content					806:812	Higher BG content	796:812	Higher BG content in the composite	796:829	Higher BG content in the composite correlated with increased human osteoblast proliferation.
27987831	4	10	theme	injectable	581:590	arg1	composites					603:612	uniform injectable paste-like composites	573:612	uniform injectable paste-like composites using BG particles and Cn	573:638	Injectability tests and scanning electron microscopy (SEM) results demonstrated the formation of uniform injectable paste-like composites using BG particles and Cn.
27987831	9	11	theme	synthesized	1149:1159	arg1	composites					1161:1170	the synthesized composites	1145:1170	the synthesized composites	1145:1170	Based on these data, the synthesized composites have a potential application in orthopedic and reconstructive surgeries as a minimally invasive bone substitute.
27987831	6	12	theme	human	857:861	arg1	proliferation					874:886	increased human osteoblast proliferation	847:886	increased human osteoblast proliferation	847:886	Higher BG content in the composite correlated with increased human osteoblast proliferation.
27987831	2	13	theme	natural	273:279	arg1	polymer					281:287	a biocompatible and osteoconductive natural polymer	237:287	a biocompatible and osteoconductive natural polymer that can promote wound healing	237:318	Chitosan (Cn) is a biocompatible and osteoconductive natural polymer that can promote wound healing.
27987831	2	13	theme	natural	273:279	arg1	Chitosan					220:227	Chitosan	220:227	Chitosan (Cn)	220:232	Chitosan (Cn) is a biocompatible and osteoconductive natural polymer that can promote wound healing.
27987831	4	14	theme	uniform	573:579	arg1	composites					603:612	uniform injectable paste-like composites	573:612	uniform injectable paste-like composites using BG particles and Cn	573:638	Injectability tests and scanning electron microscopy (SEM) results demonstrated the formation of uniform injectable paste-like composites using BG particles and Cn.
27987831	9	15	theme	orthopedic	1204:1213	arg1	surgeries					1234:1242	orthopedic and reconstructive surgeries	1204:1242	orthopedic and reconstructive surgeries	1204:1242	Based on these data, the synthesized composites have a potential application in orthopedic and reconstructive surgeries as a minimally invasive bone substitute.
27987831	2	16	theme	osteoconductive	257:271	arg1	polymer					281:287	a biocompatible and osteoconductive natural polymer	237:287	a biocompatible and osteoconductive natural polymer that can promote wound healing	237:318	Chitosan (Cn) is a biocompatible and osteoconductive natural polymer that can promote wound healing.
27987831	2	16	theme	osteoconductive	257:271	arg1	Chitosan					220:227	Chitosan	220:227	Chitosan (Cn)	220:232	Chitosan (Cn) is a biocompatible and osteoconductive natural polymer that can promote wound healing.
27987831	6	17	from	content	806:812	arg1	composite					821:829	the composite	817:829	the composite	817:829	Higher BG content in the composite correlated with increased human osteoblast proliferation.
27987831	1	18	theme	materials	164:172	arg1	materials					164:172	materials	164:172	materials that have wide applications in bone implants	164:217	SiO2-CaO-P2O5 based bioglass (BG) systems constitute a group of materials that have wide applications in bone implants.
27987831	1	18	theme	materials	164:172	arg1	group					155:159	a group	153:159	a group of materials that have wide applications in bone implants	153:217	SiO2-CaO-P2O5 based bioglass (BG) systems constitute a group of materials that have wide applications in bone implants.
27987831	8	19	theme	pathological	1048:1059	arg1	assessments					1061:1071	pathological assessments	1048:1071	pathological assessments	1048:1071	Manual palpation, radiographic images and pathological assessments proved that the composites promote bone formation.
27987831	7	20	theme	fusion	922:927	arg1	model					929:933	a rat spinal fusion model	909:933	a rat spinal fusion model	909:933	An in vivo study in a rat spinal fusion model confirmed that increasing the amount of BG improved osteoconductivity.
27987831	3	21	theme	CnB	370:372	arg1	composites					375:384	chitosan/bioglass (CnB) composites	351:384	chitosan/bioglass (CnB) composites as minimally invasive bone regenerative materials	351:434	In this study, bioactivity of chitosan/bioglass (CnB) composites as minimally invasive bone regenerative materials was assessed both in vitro and in vivo.
27987831	9	22	theme	reconstructive	1219:1232	arg1	surgeries					1234:1242	orthopedic and reconstructive surgeries	1204:1242	orthopedic and reconstructive surgeries	1204:1242	Based on these data, the synthesized composites have a potential application in orthopedic and reconstructive surgeries as a minimally invasive bone substitute.
27987831	2	23	theme	wound	306:310	arg1	healing					312:318	wound healing	306:318	wound healing	306:318	Chitosan (Cn) is a biocompatible and osteoconductive natural polymer that can promote wound healing.
27987831	3	24	theme	bone	408:411	arg1	materials					426:434	minimally invasive bone regenerative materials	389:434	minimally invasive bone regenerative materials	389:434	In this study, bioactivity of chitosan/bioglass (CnB) composites as minimally invasive bone regenerative materials was assessed both in vitro and in vivo.
27987831	7	25	theme	rat	911:913	arg1	model					929:933	a rat spinal fusion model	909:933	a rat spinal fusion model	909:933	An in vivo study in a rat spinal fusion model confirmed that increasing the amount of BG improved osteoconductivity.
27987831	1	26	theme	SiO2-CaO-P2O5	100:112	arg1	BG					130:131	BG	130:131	BG	130:131	SiO2-CaO-P2O5 based bioglass (BG) systems constitute a group of materials that have wide applications in bone implants.
27987831	1	26	theme	SiO2-CaO-P2O5	100:112	arg1	bioglass					120:127	SiO2-CaO-P2O5 based bioglass	100:127	SiO2-CaO-P2O5 based bioglass (BG) systems	100:140	SiO2-CaO-P2O5 based bioglass (BG) systems constitute a group of materials that have wide applications in bone implants.
27987831	3	27	theme	regenerative	413:424	arg1	materials					426:434	minimally invasive bone regenerative materials	389:434	minimally invasive bone regenerative materials	389:434	In this study, bioactivity of chitosan/bioglass (CnB) composites as minimally invasive bone regenerative materials was assessed both in vitro and in vivo.
27987831	3	28	theme	chitosan/bioglass	351:367	arg1	composites					375:384	chitosan/bioglass (CnB) composites	351:384	chitosan/bioglass (CnB) composites as minimally invasive bone regenerative materials	351:434	In this study, bioactivity of chitosan/bioglass (CnB) composites as minimally invasive bone regenerative materials was assessed both in vitro and in vivo.
27987831	5	29	theme	SEM	692:694	arg1	images					696:701	SEM images	692:701	SEM images	692:701	Fourier transform infrared spectroscopy (FTIR) and SEM images confirmed hydroxyapatite deposition in vitro after incubation in simulated body fluid (SBF).
27987831	7	30	theme	spinal	915:920	arg1	model					929:933	a rat spinal fusion model	909:933	a rat spinal fusion model	909:933	An in vivo study in a rat spinal fusion model confirmed that increasing the amount of BG improved osteoconductivity.
27987831	1	31	theme	based	114:118	arg1	BG					130:131	BG	130:131	BG	130:131	SiO2-CaO-P2O5 based bioglass (BG) systems constitute a group of materials that have wide applications in bone implants.
27987831	1	31	theme	based	114:118	arg1	bioglass					120:127	SiO2-CaO-P2O5 based bioglass	100:127	SiO2-CaO-P2O5 based bioglass (BG) systems	100:140	SiO2-CaO-P2O5 based bioglass (BG) systems constitute a group of materials that have wide applications in bone implants.
27987831	8	32	theme	Manual	1006:1011	arg1	palpation					1013:1021	Manual palpation	1006:1021	Manual palpation	1006:1021	Manual palpation, radiographic images and pathological assessments proved that the composites promote bone formation.
27987831	7	33	from	study	900:904	arg1	model					929:933	a rat spinal fusion model	909:933	a rat spinal fusion model	909:933	An in vivo study in a rat spinal fusion model confirmed that increasing the amount of BG improved osteoconductivity.
27987831	6	34	theme	osteoblast	863:872	arg1	proliferation					874:886	increased human osteoblast proliferation	847:886	increased human osteoblast proliferation	847:886	Higher BG content in the composite correlated with increased human osteoblast proliferation.
27987831	1	35	theme	bioglass	120:127	arg1	systems					134:140	SiO2-CaO-P2O5 based bioglass (BG) systems	100:140	SiO2-CaO-P2O5 based bioglass (BG) systems	100:140	SiO2-CaO-P2O5 based bioglass (BG) systems constitute a group of materials that have wide applications in bone implants.
27987831	0	36	theme	bioglass/chitosan	38:54	arg1	composite					56:64	a bioglass/chitosan composite	36:64	a bioglass/chitosan composite as an injectable bone	36:86	Development and characterization of a bioglass/chitosan composite as an injectable bone substitute.
27987831	8	37	theme	bone	1108:1111	arg1	formation					1113:1121	bone formation	1108:1121	bone formation	1108:1121	Manual palpation, radiographic images and pathological assessments proved that the composites promote bone formation.
27987831	1	38	contain	have	179:182	arg1	materials					164:172	materials	164:172	materials that have wide applications in bone implants	164:217	SiO2-CaO-P2O5 based bioglass (BG) systems constitute a group of materials that have wide applications in bone implants.
27987831	1	38	contain	have	179:182	arg2	applications					189:200	wide applications	184:200	wide applications	184:200	SiO2-CaO-P2O5 based bioglass (BG) systems constitute a group of materials that have wide applications in bone implants.
27987831	1	39	theme	wide	184:187	arg1	applications					189:200	wide applications	184:200	wide applications	184:200	SiO2-CaO-P2O5 based bioglass (BG) systems constitute a group of materials that have wide applications in bone implants.
27987831	1	40	theme	bone	205:208	arg1	implants					210:217	bone implants	205:217	bone implants	205:217	SiO2-CaO-P2O5 based bioglass (BG) systems constitute a group of materials that have wide applications in bone implants.
27987831	3	41	theme	invasive	399:406	arg1	materials					426:434	minimally invasive bone regenerative materials	389:434	minimally invasive bone regenerative materials	389:434	In this study, bioactivity of chitosan/bioglass (CnB) composites as minimally invasive bone regenerative materials was assessed both in vitro and in vivo.
27987831	4	42	theme	microscopy	518:527	arg1	results					535:541	scanning electron microscopy (SEM) results	500:541	scanning electron microscopy (SEM) results	500:541	Injectability tests and scanning electron microscopy (SEM) results demonstrated the formation of uniform injectable paste-like composites using BG particles and Cn.
27987831	5	43	theme	hydroxyapatite	713:726	arg1	deposition					728:737	hydroxyapatite deposition	713:737	hydroxyapatite deposition	713:737	Fourier transform infrared spectroscopy (FTIR) and SEM images confirmed hydroxyapatite deposition in vitro after incubation in simulated body fluid (SBF).
27987831	6	44	theme	increased	847:855	arg1	proliferation					874:886	increased human osteoblast proliferation	847:886	increased human osteoblast proliferation	847:886	Higher BG content in the composite correlated with increased human osteoblast proliferation.
27987831	5	45	dep	Fourier	641:647	arg1	transform					649:657	transform	649:657	transform infrared spectroscopy (FTIR) and SEM images	649:701	Fourier transform infrared spectroscopy (FTIR) and SEM images confirmed hydroxyapatite deposition in vitro after incubation in simulated body fluid (SBF).
27987831	3	46	theme	composites	375:384	arg1	bioactivity					336:346	bioactivity	336:346	bioactivity of chitosan/bioglass (CnB) composites as minimally invasive bone regenerative materials	336:434	In this study, bioactivity of chitosan/bioglass (CnB) composites as minimally invasive bone regenerative materials was assessed both in vitro and in vivo.
27987831	4	47	theme	electron	509:516	arg1	SEM					530:532	SEM	530:532	SEM	530:532	Injectability tests and scanning electron microscopy (SEM) results demonstrated the formation of uniform injectable paste-like composites using BG particles and Cn.
27987831	4	47	theme	electron	509:516	arg1	microscopy					518:527	scanning electron microscopy	500:527	scanning electron microscopy (SEM) results	500:541	Injectability tests and scanning electron microscopy (SEM) results demonstrated the formation of uniform injectable paste-like composites using BG particles and Cn.
27987831	6	48	theme	Higher	796:801	arg1	content					806:812	Higher BG content	796:812	Higher BG content in the composite	796:829	Higher BG content in the composite correlated with increased human osteoblast proliferation.
27987831	0	49	theme	composite	56:64	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Development and characterization of a bioglass/chitosan composite as an injectable bone substitute.
27987831	0	49	theme	composite	56:64	arg1	Development					0:10	Development	0:10	Development	0:10	Development and characterization of a bioglass/chitosan composite as an injectable bone substitute.
27987831	9	50	theme	substitute	1273:1282	arg1	bone					1268:1271	a minimally invasive bone	1247:1271	a minimally invasive bone substitute	1247:1282	Based on these data, the synthesized composites have a potential application in orthopedic and reconstructive surgeries as a minimally invasive bone substitute.
27987831	9	50	theme	substitute	1273:1282	arg1	application					1189:1199	a potential application	1177:1199	a potential application in orthopedic and reconstructive surgeries	1177:1242	Based on these data, the synthesized composites have a potential application in orthopedic and reconstructive surgeries as a minimally invasive bone substitute.
27987831	7	51	theme	osteoconductivity	987:1003	arg1	amount					965:970	the amount	961:970	the amount of BG improved osteoconductivity	961:1003	An in vivo study in a rat spinal fusion model confirmed that increasing the amount of BG improved osteoconductivity.
27987831	7	51	theme	osteoconductivity	987:1003	arg1	osteoconductivity					987:1003	BG improved osteoconductivity	975:1003	BG improved osteoconductivity	975:1003	An in vivo study in a rat spinal fusion model confirmed that increasing the amount of BG improved osteoconductivity.
27987831	9	52	theme	invasive	1259:1266	arg1	bone					1268:1271	a minimally invasive bone	1247:1271	a minimally invasive bone substitute	1247:1282	Based on these data, the synthesized composites have a potential application in orthopedic and reconstructive surgeries as a minimally invasive bone substitute.
27987831	9	52	theme	invasive	1259:1266	arg1	application					1189:1199	a potential application	1177:1199	a potential application in orthopedic and reconstructive surgeries	1177:1242	Based on these data, the synthesized composites have a potential application in orthopedic and reconstructive surgeries as a minimally invasive bone substitute.
27987831	7	53	dep	in	892:893	arg1	vivo					895:898	vivo	895:898	vivo	895:898	An in vivo study in a rat spinal fusion model confirmed that increasing the amount of BG improved osteoconductivity.
27987831	2	54	theme	biocompatible	239:251	arg1	polymer					281:287	a biocompatible and osteoconductive natural polymer	237:287	a biocompatible and osteoconductive natural polymer that can promote wound healing	237:318	Chitosan (Cn) is a biocompatible and osteoconductive natural polymer that can promote wound healing.
27987831	2	54	theme	biocompatible	239:251	arg1	Chitosan					220:227	Chitosan	220:227	Chitosan (Cn)	220:232	Chitosan (Cn) is a biocompatible and osteoconductive natural polymer that can promote wound healing.
27987831	4	55	theme	composites	603:612	arg1	formation					560:568	the formation	556:568	the formation of uniform injectable paste-like composites using BG particles and Cn	556:638	Injectability tests and scanning electron microscopy (SEM) results demonstrated the formation of uniform injectable paste-like composites using BG particles and Cn.
27987831	5	56	from	incubation	754:763	arg1	SBF					790:792	SBF	790:792	SBF	790:792	Fourier transform infrared spectroscopy (FTIR) and SEM images confirmed hydroxyapatite deposition in vitro after incubation in simulated body fluid (SBF).
27987831	5	56	from	incubation	754:763	arg1	fluid					783:787	simulated body fluid	768:787	simulated body fluid (SBF)	768:793	Fourier transform infrared spectroscopy (FTIR) and SEM images confirmed hydroxyapatite deposition in vitro after incubation in simulated body fluid (SBF).
27987831	7	57	theme	BG	975:976	arg1	osteoconductivity					987:1003	BG improved osteoconductivity	975:1003	BG improved osteoconductivity	975:1003	An in vivo study in a rat spinal fusion model confirmed that increasing the amount of BG improved osteoconductivity.
27987831	4	58	theme	paste-like	592:601	arg1	composites					603:612	uniform injectable paste-like composites	573:612	uniform injectable paste-like composites using BG particles and Cn	573:638	Injectability tests and scanning electron microscopy (SEM) results demonstrated the formation of uniform injectable paste-like composites using BG particles and Cn.
27987831	0	59	theme	injectable	72:81	arg1	bone					83:86	an injectable bone	69:86	an injectable bone	69:86	Development and characterization of a bioglass/chitosan composite as an injectable bone substitute.
27987831	7	60	theme	improved	978:985	arg1	osteoconductivity					987:1003	BG improved osteoconductivity	975:1003	BG improved osteoconductivity	975:1003	An in vivo study in a rat spinal fusion model confirmed that increasing the amount of BG improved osteoconductivity.
27987831	4	61	theme	BG	620:621	arg1	particles					623:631	BG particles	620:631	BG particles	620:631	Injectability tests and scanning electron microscopy (SEM) results demonstrated the formation of uniform injectable paste-like composites using BG particles and Cn.
27987831	9	62	contain	have	1172:1175	arg1	composites					1161:1170	the synthesized composites	1145:1170	the synthesized composites	1145:1170	Based on these data, the synthesized composites have a potential application in orthopedic and reconstructive surgeries as a minimally invasive bone substitute.
27987831	9	62	contain	have	1172:1175	arg2	bone					1268:1271	a minimally invasive bone	1247:1271	a minimally invasive bone substitute	1247:1282	Based on these data, the synthesized composites have a potential application in orthopedic and reconstructive surgeries as a minimally invasive bone substitute.
27987831	9	62	contain	have	1172:1175	arg2	application					1189:1199	a potential application	1177:1199	a potential application in orthopedic and reconstructive surgeries	1177:1242	Based on these data, the synthesized composites have a potential application in orthopedic and reconstructive surgeries as a minimally invasive bone substitute.
24512093	0	0	theme	single-wall	96:106	arg1	nanotubes					115:123	single-wall carbon nanotubes	96:123	single-wall carbon nanotubes	96:123	Highly conducting, strong nanocomposites based on nanocellulose-assisted aqueous dispersions of single-wall carbon nanotubes.
24512093	3	1	theme	increased	497:505	arg1	cost					507:510	increased cost	497:510	increased cost	497:510	This leads to increased cost, a loss of strength and lower conductivity.
24512093	3	1	theme	increased	497:505	arg1	loss					515:518	a loss	513:518	a loss of strength and lower conductivity	513:553	This leads to increased cost, a loss of strength and lower conductivity.
24512093	4	2	theme	full	568:571	arg1	potential					573:581	full potential	568:581	full potential of colloidal self-assembly	568:608	In addition full potential of colloidal self-assembly cannot be fully exploited in a polymer matrix.
24512093	10	3	theme	S	1429:1429	arg1	-1					1434:1435	-1	1434:1435	-1	1434:1435	The anisotropic microfiber composites have maximum conductivities above 200 S cm(-1) and current densities reaching 1400 A cm(-2).
24512093	10	3	theme	S	1429:1429	arg1	cm					1431:1432	200 S cm	1425:1432	200 S cm(-1)	1425:1436	The anisotropic microfiber composites have maximum conductivities above 200 S cm(-1) and current densities reaching 1400 A cm(-2).
24512093	8	4	with	aerogels	1151:1158	arg1	structure					1219:1227	nanoscale composite structure	1199:1227	nanoscale composite structure	1199:1227	We demonstrate semitransparent conductive films, aerogels and anisotropic microscale fibers with nanoscale composite structure.
24512093	1	5	theme	single-wall	182:192	arg1	SWNTs					205:209	SWNTs	205:209	SWNTs	205:209	It is challenging to obtain high-quality dispersions of single-wall nanotubes (SWNTs) in composite matrix materials, in order to reach the full potential of mechanical and electronic properties.
24512093	1	5	theme	single-wall	182:192	arg1	nanotubes					194:202	single-wall nanotubes	182:202	single-wall nanotubes (SWNTs)	182:210	It is challenging to obtain high-quality dispersions of single-wall nanotubes (SWNTs) in composite matrix materials, in order to reach the full potential of mechanical and electronic properties.
24512093	9	6	theme	SWNT	1238:1241	arg1	nanopaper					1243:1251	SWNT nanopaper	1238:1251	SWNT nanopaper	1238:1251	The NFC:SWNT nanopaper shows increased strength at 3 wt % SWNT, reaching a modulus of 13.3 GPa, and a strength of 307 MPa.
24512093	0	7	theme	nanotubes	115:123	arg1	dispersions					81:91	nanocellulose-assisted aqueous dispersions	50:91	nanocellulose-assisted aqueous dispersions of single-wall carbon nanotubes	50:123	Highly conducting, strong nanocomposites based on nanocellulose-assisted aqueous dispersions of single-wall carbon nanotubes.
24512093	10	8	theme	anisotropic	1357:1367	arg1	composites					1380:1389	The anisotropic microfiber composites	1353:1389	The anisotropic microfiber composites	1353:1389	The anisotropic microfiber composites have maximum conductivities above 200 S cm(-1) and current densities reaching 1400 A cm(-2).
24512093	3	9	theme	strength	523:530	arg1	cost					507:510	increased cost	497:510	increased cost	497:510	This leads to increased cost, a loss of strength and lower conductivity.
24512093	3	9	theme	strength	523:530	arg1	loss					515:518	a loss	513:518	a loss of strength and lower conductivity	513:553	This leads to increased cost, a loss of strength and lower conductivity.
24512093	1	10	theme	full	265:268	arg1	potential					270:278	the full potential	261:278	the full potential of mechanical and electronic properties	261:318	It is challenging to obtain high-quality dispersions of single-wall nanotubes (SWNTs) in composite matrix materials, in order to reach the full potential of mechanical and electronic properties.
24512093	0	11	theme	carbon	108:113	arg1	nanotubes					115:123	single-wall carbon nanotubes	96:123	single-wall carbon nanotubes	96:123	Highly conducting, strong nanocomposites based on nanocellulose-assisted aqueous dispersions of single-wall carbon nanotubes.
24512093	6	12	theme	low-cost	886:893	arg1	exfoliation					895:905	possible low-cost exfoliation	877:905	possible low-cost exfoliation	877:905	Here we show that nanofibrillated cellulose (NFC) can act as an excellent aqueous dispersion agent for as-prepared SWNTs, making possible low-cost exfoliation and purification of SWNTs with dispersion limits exceeding 40 wt %.
24512093	8	13	with	films	1144:1148	arg1	structure					1219:1227	nanoscale composite structure	1199:1227	nanoscale composite structure	1199:1227	We demonstrate semitransparent conductive films, aerogels and anisotropic microscale fibers with nanoscale composite structure.
24512093	8	14	theme	conductive	1133:1142	arg1	films					1144:1148	semitransparent conductive films	1117:1148	semitransparent conductive films	1117:1148	We demonstrate semitransparent conductive films, aerogels and anisotropic microscale fibers with nanoscale composite structure.
24512093	5	15	theme	nanocomposites	732:745	arg1	assembly					694:701	assembly	694:701	assembly of highly ordered structural nanocomposites	694:745	This may limit the possibilities for assembly of highly ordered structural nanocomposites.
24512093	6	16	theme	possible	877:884	arg1	exfoliation					895:905	possible low-cost exfoliation	877:905	possible low-cost exfoliation	877:905	Here we show that nanofibrillated cellulose (NFC) can act as an excellent aqueous dispersion agent for as-prepared SWNTs, making possible low-cost exfoliation and purification of SWNTs with dispersion limits exceeding 40 wt %.
24512093	7	17	theme	advanced	1081:1088	arg1	composites					1090:1099	advanced composites	1081:1099	advanced composites	1081:1099	The NFC:SWNT dispersion may also offer a cheap and sustainable alternative for molecular self-assembly of advanced composites.
24512093	1	18	from	dispersions	167:177	arg1	materials					232:240	composite matrix materials	215:240	composite matrix materials	215:240	It is challenging to obtain high-quality dispersions of single-wall nanotubes (SWNTs) in composite matrix materials, in order to reach the full potential of mechanical and electronic properties.
24512093	1	19	theme	nanotubes	194:202	arg1	dispersions					167:177	high-quality dispersions	154:177	high-quality dispersions of single-wall nanotubes (SWNTs) in composite matrix materials	154:240	It is challenging to obtain high-quality dispersions of single-wall nanotubes (SWNTs) in composite matrix materials, in order to reach the full potential of mechanical and electronic properties.
24512093	8	20	theme	microscale	1176:1185	arg1	fibers					1187:1192	anisotropic microscale fibers	1164:1192	anisotropic microscale fibers with nanoscale composite structure	1164:1227	We demonstrate semitransparent conductive films, aerogels and anisotropic microscale fibers with nanoscale composite structure.
24512093	7	21	theme	composites	1090:1099	arg1	self-assembly					1064:1076	molecular self-assembly	1054:1076	molecular self-assembly of advanced composites	1054:1099	The NFC:SWNT dispersion may also offer a cheap and sustainable alternative for molecular self-assembly of advanced composites.
24512093	8	22	with	fibers	1187:1192	arg1	structure					1219:1227	nanoscale composite structure	1199:1227	nanoscale composite structure	1199:1227	We demonstrate semitransparent conductive films, aerogels and anisotropic microscale fibers with nanoscale composite structure.
24512093	4	23	theme	polymer	641:647	arg1	matrix					649:654	a polymer matrix	639:654	a polymer matrix	639:654	In addition full potential of colloidal self-assembly cannot be fully exploited in a polymer matrix.
24512093	2	24	theme	chemical	443:450	arg1	route					381:385	the route	377:385	the route to achieving high quality dispersions of SWNT	377:431	The most widely used matrix materials are polymers, and the route to achieving high quality dispersions of SWNT is mainly chemical functionalization of the SWNT.
24512093	2	24	theme	chemical	443:450	arg1	functionalization					452:468	chemical functionalization	443:468	chemical functionalization of the SWNT	443:480	The most widely used matrix materials are polymers, and the route to achieving high quality dispersions of SWNT is mainly chemical functionalization of the SWNT.
24512093	10	25	dep	cm	1431:1432	arg1	cm					1476:1477	A cm	1474:1477	A cm(-2)	1474:1481	The anisotropic microfiber composites have maximum conductivities above 200 S cm(-1) and current densities reaching 1400 A cm(-2).
24512093	10	25	dep	cm	1431:1432	arg1	-2					1479:1480	-2	1479:1480	-2	1479:1480	The anisotropic microfiber composites have maximum conductivities above 200 S cm(-1) and current densities reaching 1400 A cm(-2).
24512093	10	26	theme	maximum	1396:1402	arg1	conductivities					1404:1417	maximum conductivities	1396:1417	maximum conductivities	1396:1417	The anisotropic microfiber composites have maximum conductivities above 200 S cm(-1) and current densities reaching 1400 A cm(-2).
24512093	9	27	theme	GPa	1321:1323	arg1	modulus					1305:1311	a modulus	1303:1311	a modulus of 13.3 GPa	1303:1323	The NFC:SWNT nanopaper shows increased strength at 3 wt % SWNT, reaching a modulus of 13.3 GPa, and a strength of 307 MPa.
24512093	9	27	theme	GPa	1321:1323	arg1	strength					1332:1339	a strength	1330:1339	a strength of 307 MPa	1330:1350	The NFC:SWNT nanopaper shows increased strength at 3 wt % SWNT, reaching a modulus of 13.3 GPa, and a strength of 307 MPa.
24512093	10	28	contain	have	1391:1394	arg2	conductivities					1404:1417	maximum conductivities	1396:1417	maximum conductivities	1396:1417	The anisotropic microfiber composites have maximum conductivities above 200 S cm(-1) and current densities reaching 1400 A cm(-2).
24512093	10	28	contain	have	1391:1394	arg1	composites					1380:1389	The anisotropic microfiber composites	1353:1389	The anisotropic microfiber composites	1353:1389	The anisotropic microfiber composites have maximum conductivities above 200 S cm(-1) and current densities reaching 1400 A cm(-2).
24512093	6	29	theme	as-prepared	851:861	arg1	SWNTs					863:867	as-prepared SWNTs	851:867	as-prepared SWNTs	851:867	Here we show that nanofibrillated cellulose (NFC) can act as an excellent aqueous dispersion agent for as-prepared SWNTs, making possible low-cost exfoliation and purification of SWNTs with dispersion limits exceeding 40 wt %.
24512093	1	30	theme	mechanical	283:292	arg1	properties					309:318	mechanical and electronic properties	283:318	properties	309:318	It is challenging to obtain high-quality dispersions of single-wall nanotubes (SWNTs) in composite matrix materials, in order to reach the full potential of mechanical and electronic properties.
24512093	6	31	theme	nanofibrillated	766:780	arg1	NFC					793:795	NFC	793:795	NFC	793:795	Here we show that nanofibrillated cellulose (NFC) can act as an excellent aqueous dispersion agent for as-prepared SWNTs, making possible low-cost exfoliation and purification of SWNTs with dispersion limits exceeding 40 wt %.
24512093	6	31	theme	nanofibrillated	766:780	arg1	cellulose					782:790	nanofibrillated cellulose	766:790	nanofibrillated cellulose (NFC)	766:796	Here we show that nanofibrillated cellulose (NFC) can act as an excellent aqueous dispersion agent for as-prepared SWNTs, making possible low-cost exfoliation and purification of SWNTs with dispersion limits exceeding 40 wt %.
24512093	6	31	theme	nanofibrillated	766:780	arg1	agent					841:845	an excellent aqueous dispersion agent	809:845	an excellent aqueous dispersion agent for as-prepared SWNTs	809:867	Here we show that nanofibrillated cellulose (NFC) can act as an excellent aqueous dispersion agent for as-prepared SWNTs, making possible low-cost exfoliation and purification of SWNTs with dispersion limits exceeding 40 wt %.
24512093	3	32	theme	lower	536:540	arg1	conductivity					542:553	lower conductivity	536:553	lower conductivity	536:553	This leads to increased cost, a loss of strength and lower conductivity.
24512093	10	33	theme	microfiber	1369:1378	arg1	composites					1380:1389	The anisotropic microfiber composites	1353:1389	The anisotropic microfiber composites	1353:1389	The anisotropic microfiber composites have maximum conductivities above 200 S cm(-1) and current densities reaching 1400 A cm(-2).
24512093	2	34	theme	SWNT	428:431	arg1	dispersions					413:423	achieving high quality dispersions	390:423	achieving high quality dispersions of SWNT	390:431	The most widely used matrix materials are polymers, and the route to achieving high quality dispersions of SWNT is mainly chemical functionalization of the SWNT.
24512093	7	35	theme	molecular	1054:1062	arg1	self-assembly					1064:1076	molecular self-assembly	1054:1076	molecular self-assembly of advanced composites	1054:1099	The NFC:SWNT dispersion may also offer a cheap and sustainable alternative for molecular self-assembly of advanced composites.
24512093	9	36	theme	increased	1259:1267	arg1	strength					1269:1276	increased strength	1259:1276	increased strength at 3 wt % SWNT	1259:1291	The NFC:SWNT nanopaper shows increased strength at 3 wt % SWNT, reaching a modulus of 13.3 GPa, and a strength of 307 MPa.
24512093	3	37	theme	conductivity	542:553	arg1	cost					507:510	increased cost	497:510	increased cost	497:510	This leads to increased cost, a loss of strength and lower conductivity.
24512093	3	37	theme	conductivity	542:553	arg1	loss					515:518	a loss	513:518	a loss of strength and lower conductivity	513:553	This leads to increased cost, a loss of strength and lower conductivity.
24512093	8	38	theme	anisotropic	1164:1174	arg1	fibers					1187:1192	anisotropic microscale fibers	1164:1192	anisotropic microscale fibers with nanoscale composite structure	1164:1227	We demonstrate semitransparent conductive films, aerogels and anisotropic microscale fibers with nanoscale composite structure.
24512093	1	39	theme	composite	215:223	arg1	materials					232:240	composite matrix materials	215:240	composite matrix materials	215:240	It is challenging to obtain high-quality dispersions of single-wall nanotubes (SWNTs) in composite matrix materials, in order to reach the full potential of mechanical and electronic properties.
24512093	2	40	theme	SWNT	477:480	arg1	polymers					363:370	polymers	363:370	polymers	363:370	The most widely used matrix materials are polymers, and the route to achieving high quality dispersions of SWNT is mainly chemical functionalization of the SWNT.
24512093	2	40	theme	SWNT	477:480	arg1	materials					349:357	The most widely used matrix materials	321:357	The most widely used matrix materials	321:357	The most widely used matrix materials are polymers, and the route to achieving high quality dispersions of SWNT is mainly chemical functionalization of the SWNT.
24512093	2	40	theme	SWNT	477:480	arg1	route					381:385	the route	377:385	the route to achieving high quality dispersions of SWNT	377:431	The most widely used matrix materials are polymers, and the route to achieving high quality dispersions of SWNT is mainly chemical functionalization of the SWNT.
24512093	2	40	theme	SWNT	477:480	arg1	functionalization					452:468	chemical functionalization	443:468	chemical functionalization of the SWNT	443:480	The most widely used matrix materials are polymers, and the route to achieving high quality dispersions of SWNT is mainly chemical functionalization of the SWNT.
24512093	1	41	theme	electronic	298:307	arg1	properties					309:318	mechanical and electronic properties	283:318	properties	309:318	It is challenging to obtain high-quality dispersions of single-wall nanotubes (SWNTs) in composite matrix materials, in order to reach the full potential of mechanical and electronic properties.
24512093	7	42	theme	sustainable	1026:1036	arg1	alternative					1038:1048	a cheap and sustainable alternative	1014:1048	a cheap and sustainable alternative for molecular self-assembly of advanced composites	1014:1099	The NFC:SWNT dispersion may also offer a cheap and sustainable alternative for molecular self-assembly of advanced composites.
24512093	1	43	theme	matrix	225:230	arg1	materials					232:240	composite matrix materials	215:240	composite matrix materials	215:240	It is challenging to obtain high-quality dispersions of single-wall nanotubes (SWNTs) in composite matrix materials, in order to reach the full potential of mechanical and electronic properties.
24512093	8	44	theme	nanoscale	1199:1207	arg1	structure					1219:1227	nanoscale composite structure	1199:1227	nanoscale composite structure	1199:1227	We demonstrate semitransparent conductive films, aerogels and anisotropic microscale fibers with nanoscale composite structure.
24512093	2	45	theme	quality	405:411	arg1	dispersions					413:423	achieving high quality dispersions	390:423	achieving high quality dispersions of SWNT	390:431	The most widely used matrix materials are polymers, and the route to achieving high quality dispersions of SWNT is mainly chemical functionalization of the SWNT.
24512093	2	46	theme	achieving	390:398	arg1	dispersions					413:423	achieving high quality dispersions	390:423	achieving high quality dispersions of SWNT	390:431	The most widely used matrix materials are polymers, and the route to achieving high quality dispersions of SWNT is mainly chemical functionalization of the SWNT.
24512093	1	47	theme	properties	309:318	arg1	potential					270:278	the full potential	261:278	the full potential of mechanical and electronic properties	261:318	It is challenging to obtain high-quality dispersions of single-wall nanotubes (SWNTs) in composite matrix materials, in order to reach the full potential of mechanical and electronic properties.
24512093	9	48	theme	MPa	1348:1350	arg1	modulus					1305:1311	a modulus	1303:1311	a modulus of 13.3 GPa	1303:1323	The NFC:SWNT nanopaper shows increased strength at 3 wt % SWNT, reaching a modulus of 13.3 GPa, and a strength of 307 MPa.
24512093	9	48	theme	MPa	1348:1350	arg1	strength					1332:1339	a strength	1330:1339	a strength of 307 MPa	1330:1350	The NFC:SWNT nanopaper shows increased strength at 3 wt % SWNT, reaching a modulus of 13.3 GPa, and a strength of 307 MPa.
24512093	10	49	theme	current	1442:1448	arg1	densities					1450:1458	current densities	1442:1458	current densities reaching 1400	1442:1472	The anisotropic microfiber composites have maximum conductivities above 200 S cm(-1) and current densities reaching 1400 A cm(-2).
24512093	2	50	theme	high	400:403	arg1	dispersions					413:423	achieving high quality dispersions	390:423	achieving high quality dispersions of SWNT	390:431	The most widely used matrix materials are polymers, and the route to achieving high quality dispersions of SWNT is mainly chemical functionalization of the SWNT.
24512093	0	51	theme	nanocellulose-assisted	50:71	arg1	dispersions					81:91	nanocellulose-assisted aqueous dispersions	50:91	nanocellulose-assisted aqueous dispersions of single-wall carbon nanotubes	50:123	Highly conducting, strong nanocomposites based on nanocellulose-assisted aqueous dispersions of single-wall carbon nanotubes.
24512093	5	52	theme	ordered	713:719	arg1	nanocomposites					732:745	highly ordered structural nanocomposites	706:745	highly ordered structural nanocomposites	706:745	This may limit the possibilities for assembly of highly ordered structural nanocomposites.
24512093	7	53	theme	cheap	1016:1020	arg1	alternative					1038:1048	a cheap and sustainable alternative	1014:1048	a cheap and sustainable alternative for molecular self-assembly of advanced composites	1014:1099	The NFC:SWNT dispersion may also offer a cheap and sustainable alternative for molecular self-assembly of advanced composites.
24512093	6	54	theme	wt	969:970	arg1	%					972:972	40 wt %	966:972	40 wt %	966:972	Here we show that nanofibrillated cellulose (NFC) can act as an excellent aqueous dispersion agent for as-prepared SWNTs, making possible low-cost exfoliation and purification of SWNTs with dispersion limits exceeding 40 wt %.
24512093	9	55	from	SWNT	1288:1291	arg1	strength					1269:1276	increased strength	1259:1276	increased strength at 3 wt % SWNT	1259:1291	The NFC:SWNT nanopaper shows increased strength at 3 wt % SWNT, reaching a modulus of 13.3 GPa, and a strength of 307 MPa.
24512093	6	56	theme	dispersion	830:839	arg1	cellulose					782:790	nanofibrillated cellulose	766:790	nanofibrillated cellulose (NFC)	766:796	Here we show that nanofibrillated cellulose (NFC) can act as an excellent aqueous dispersion agent for as-prepared SWNTs, making possible low-cost exfoliation and purification of SWNTs with dispersion limits exceeding 40 wt %.
24512093	6	56	theme	dispersion	830:839	arg1	agent					841:845	an excellent aqueous dispersion agent	809:845	an excellent aqueous dispersion agent for as-prepared SWNTs	809:867	Here we show that nanofibrillated cellulose (NFC) can act as an excellent aqueous dispersion agent for as-prepared SWNTs, making possible low-cost exfoliation and purification of SWNTs with dispersion limits exceeding 40 wt %.
24512093	7	57	theme	SWNT	983:986	arg1	dispersion					988:997	The NFC:SWNT dispersion	975:997	The NFC:SWNT dispersion	975:997	The NFC:SWNT dispersion may also offer a cheap and sustainable alternative for molecular self-assembly of advanced composites.
24512093	2	58	theme	matrix	342:347	arg1	polymers					363:370	polymers	363:370	polymers	363:370	The most widely used matrix materials are polymers, and the route to achieving high quality dispersions of SWNT is mainly chemical functionalization of the SWNT.
24512093	2	58	theme	matrix	342:347	arg1	materials					349:357	The most widely used matrix materials	321:357	The most widely used matrix materials	321:357	The most widely used matrix materials are polymers, and the route to achieving high quality dispersions of SWNT is mainly chemical functionalization of the SWNT.
24512093	6	59	theme	SWNTs	927:931	arg1	purification					911:922	purification	911:922	purification	911:922	Here we show that nanofibrillated cellulose (NFC) can act as an excellent aqueous dispersion agent for as-prepared SWNTs, making possible low-cost exfoliation and purification of SWNTs with dispersion limits exceeding 40 wt %.
24512093	6	59	theme	SWNTs	927:931	arg1	exfoliation					895:905	possible low-cost exfoliation	877:905	possible low-cost exfoliation	877:905	Here we show that nanofibrillated cellulose (NFC) can act as an excellent aqueous dispersion agent for as-prepared SWNTs, making possible low-cost exfoliation and purification of SWNTs with dispersion limits exceeding 40 wt %.
24512093	8	60	theme	semitransparent	1117:1131	arg1	films					1144:1148	semitransparent conductive films	1117:1148	semitransparent conductive films	1117:1148	We demonstrate semitransparent conductive films, aerogels and anisotropic microscale fibers with nanoscale composite structure.
24512093	6	61	theme	aqueous	822:828	arg1	cellulose					782:790	nanofibrillated cellulose	766:790	nanofibrillated cellulose (NFC)	766:796	Here we show that nanofibrillated cellulose (NFC) can act as an excellent aqueous dispersion agent for as-prepared SWNTs, making possible low-cost exfoliation and purification of SWNTs with dispersion limits exceeding 40 wt %.
24512093	6	61	theme	aqueous	822:828	arg1	agent					841:845	an excellent aqueous dispersion agent	809:845	an excellent aqueous dispersion agent for as-prepared SWNTs	809:867	Here we show that nanofibrillated cellulose (NFC) can act as an excellent aqueous dispersion agent for as-prepared SWNTs, making possible low-cost exfoliation and purification of SWNTs with dispersion limits exceeding 40 wt %.
24512093	2	62	theme	used	337:340	arg1	polymers					363:370	polymers	363:370	polymers	363:370	The most widely used matrix materials are polymers, and the route to achieving high quality dispersions of SWNT is mainly chemical functionalization of the SWNT.
24512093	2	62	theme	used	337:340	arg1	materials					349:357	The most widely used matrix materials	321:357	The most widely used matrix materials	321:357	The most widely used matrix materials are polymers, and the route to achieving high quality dispersions of SWNT is mainly chemical functionalization of the SWNT.
24512093	4	63	theme	self-assembly	596:608	arg1	potential					573:581	full potential	568:581	full potential of colloidal self-assembly	568:608	In addition full potential of colloidal self-assembly cannot be fully exploited in a polymer matrix.
24512093	6	64	theme	excellent	812:820	arg1	cellulose					782:790	nanofibrillated cellulose	766:790	nanofibrillated cellulose (NFC)	766:796	Here we show that nanofibrillated cellulose (NFC) can act as an excellent aqueous dispersion agent for as-prepared SWNTs, making possible low-cost exfoliation and purification of SWNTs with dispersion limits exceeding 40 wt %.
24512093	6	64	theme	excellent	812:820	arg1	agent					841:845	an excellent aqueous dispersion agent	809:845	an excellent aqueous dispersion agent for as-prepared SWNTs	809:867	Here we show that nanofibrillated cellulose (NFC) can act as an excellent aqueous dispersion agent for as-prepared SWNTs, making possible low-cost exfoliation and purification of SWNTs with dispersion limits exceeding 40 wt %.
24512093	7	65	theme	NFC	979:981	arg1	dispersion					988:997	The NFC:SWNT dispersion	975:997	The NFC:SWNT dispersion	975:997	The NFC:SWNT dispersion may also offer a cheap and sustainable alternative for molecular self-assembly of advanced composites.
24512093	4	66	theme	colloidal	586:594	arg1	self-assembly					596:608	colloidal self-assembly	586:608	colloidal self-assembly	586:608	In addition full potential of colloidal self-assembly cannot be fully exploited in a polymer matrix.
24512093	9	67	theme	wt	1283:1284	arg1	SWNT					1288:1291	3 wt % SWNT	1281:1291	3 wt % SWNT	1281:1291	The NFC:SWNT nanopaper shows increased strength at 3 wt % SWNT, reaching a modulus of 13.3 GPa, and a strength of 307 MPa.
24512093	0	68	dep	conducting	7:16	arg1	strong					19:24	strong	19:24	strong	19:24	Highly conducting, strong nanocomposites based on nanocellulose-assisted aqueous dispersions of single-wall carbon nanotubes.
24512093	9	69	dep	NFC	1234:1236	arg1	shows					1253:1257	shows	1253:1257	shows	1253:1257	The NFC:SWNT nanopaper shows increased strength at 3 wt % SWNT, reaching a modulus of 13.3 GPa, and a strength of 307 MPa.
24512093	1	70	theme	high-quality	154:165	arg1	dispersions					167:177	high-quality dispersions	154:177	high-quality dispersions of single-wall nanotubes (SWNTs) in composite matrix materials	154:240	It is challenging to obtain high-quality dispersions of single-wall nanotubes (SWNTs) in composite matrix materials, in order to reach the full potential of mechanical and electronic properties.
24512093	0	71	theme	aqueous	73:79	arg1	dispersions					81:91	nanocellulose-assisted aqueous dispersions	50:91	nanocellulose-assisted aqueous dispersions of single-wall carbon nanotubes	50:123	Highly conducting, strong nanocomposites based on nanocellulose-assisted aqueous dispersions of single-wall carbon nanotubes.
24512093	9	72	theme	%	1286:1286	arg1	SWNT					1288:1291	3 wt % SWNT	1281:1291	3 wt % SWNT	1281:1291	The NFC:SWNT nanopaper shows increased strength at 3 wt % SWNT, reaching a modulus of 13.3 GPa, and a strength of 307 MPa.
24512093	8	73	theme	composite	1209:1217	arg1	structure					1219:1227	nanoscale composite structure	1199:1227	nanoscale composite structure	1199:1227	We demonstrate semitransparent conductive films, aerogels and anisotropic microscale fibers with nanoscale composite structure.
24512093	0	74	theme	conducting	7:16	arg1	nanocomposites					26:39	Highly conducting, strong nanocomposites	0:39	Highly conducting, strong nanocomposites	0:39	Highly conducting, strong nanocomposites based on nanocellulose-assisted aqueous dispersions of single-wall carbon nanotubes.
24512093	6	75	theme	dispersion	938:947	arg1	limits					949:954	dispersion limits	938:954	dispersion limits exceeding 40 wt %	938:972	Here we show that nanofibrillated cellulose (NFC) can act as an excellent aqueous dispersion agent for as-prepared SWNTs, making possible low-cost exfoliation and purification of SWNTs with dispersion limits exceeding 40 wt %.
24512093	5	76	theme	structural	721:730	arg1	nanocomposites					732:745	highly ordered structural nanocomposites	706:745	highly ordered structural nanocomposites	706:745	This may limit the possibilities for assembly of highly ordered structural nanocomposites.
23666851	4	0	theme	1	496:496	arg1	ratios					486:491	collagen weight ratios	470:491	collagen weight ratios of 1, 3, 5, 7, and 10	470:513	A series of collagen/CNCs films were prepared by collagen solution with CNCs suspensions homogeneously dispersed at CNCs: collagen weight ratios of 1, 3, 5, 7, and 10.
23666851	7	1	theme	in	912:913	arg1	studies					934:940	in vitro cell culture studies	912:940	in vitro cell culture studies	912:940	In addition, in vitro cell culture studies revealed that the CNCs have no negative effect on the cell morphology, viability, and proliferation and possess good biocompatibility.
23666851	1	2	theme	engineering	154:164	arg1	field					134:138	the field	130:138	the field of biomedical engineering	130:164	Collagen films have been widely used in the field of biomedical engineering.
23666851	6	3	theme	mechanical	838:847	arg1	properties					849:858	the maximum mechanical properties	826:858	the maximum mechanical properties for the collagen/CNCs composite films	826:896	Among the CNCs contents used, a loading of 7 wt % led to the maximum mechanical properties for the collagen/CNCs composite films.
23666851	6	4	theme	maximum	830:836	arg1	properties					849:858	the maximum mechanical properties	826:858	the maximum mechanical properties for the collagen/CNCs composite films	826:896	Among the CNCs contents used, a loading of 7 wt % led to the maximum mechanical properties for the collagen/CNCs composite films.
23666851	3	5	used	used	314:317	arg2	CNCs					288:291	CNCs	288:291	CNCs	288:291	Here, rod-like cellulose nanocrystals (CNCs) were fabricated and used to reinforce collagen films.
23666851	3	5	used	used	314:317	arg2	nanocrystals					274:285	rod-like cellulose nanocrystals	255:285	rod-like cellulose nanocrystals (CNCs)	255:292	Here, rod-like cellulose nanocrystals (CNCs) were fabricated and used to reinforce collagen films.
23666851	5	6	theme	films	548:552	arg1	morphology					520:529	The morphology	516:529	The morphology of the resulting films	516:552	The morphology of the resulting films was analyzed by scanning electron microscopy (SEM), the enhancement of the thermomechanical properties of the collagen/CNCs composites were demonstrated by thermal gravimetric analysis (TGA) and mechanical testing.
23666851	5	7	theme	composites	678:687	arg1	properties					646:655	the thermomechanical properties	625:655	the thermomechanical properties of the collagen/CNCs composites	625:687	The morphology of the resulting films was analyzed by scanning electron microscopy (SEM), the enhancement of the thermomechanical properties of the collagen/CNCs composites were demonstrated by thermal gravimetric analysis (TGA) and mechanical testing.
23666851	7	8	contain	possess	1046:1052	arg1	CNCs					960:963	the CNCs	956:963	the CNCs	956:963	In addition, in vitro cell culture studies revealed that the CNCs have no negative effect on the cell morphology, viability, and proliferation and possess good biocompatibility.
23666851	7	8	contain	possess	1046:1052	arg2	biocompatibility					1059:1074	good biocompatibility	1054:1074	good biocompatibility	1054:1074	In addition, in vitro cell culture studies revealed that the CNCs have no negative effect on the cell morphology, viability, and proliferation and possess good biocompatibility.
23666851	4	9	theme	weight	479:484	arg1	ratios					486:491	collagen weight ratios	470:491	collagen weight ratios of 1, 3, 5, 7, and 10	470:513	A series of collagen/CNCs films were prepared by collagen solution with CNCs suspensions homogeneously dispersed at CNCs: collagen weight ratios of 1, 3, 5, 7, and 10.
23666851	4	10	theme	collagen	470:477	arg1	ratios					486:491	collagen weight ratios	470:491	collagen weight ratios of 1, 3, 5, 7, and 10	470:513	A series of collagen/CNCs films were prepared by collagen solution with CNCs suspensions homogeneously dispersed at CNCs: collagen weight ratios of 1, 3, 5, 7, and 10.
23666851	5	11	theme	scanning	570:577	arg1	microscopy					588:597	scanning electron microscopy	570:597	scanning electron microscopy (SEM)	570:603	The morphology of the resulting films was analyzed by scanning electron microscopy (SEM), the enhancement of the thermomechanical properties of the collagen/CNCs composites were demonstrated by thermal gravimetric analysis (TGA) and mechanical testing.
23666851	5	11	theme	scanning	570:577	arg1	SEM					600:602	SEM	600:602	SEM	600:602	The morphology of the resulting films was analyzed by scanning electron microscopy (SEM), the enhancement of the thermomechanical properties of the collagen/CNCs composites were demonstrated by thermal gravimetric analysis (TGA) and mechanical testing.
23666851	4	12	theme	collagen	397:404	arg1	solution					406:413	collagen solution	397:413	collagen solution with CNCs suspensions homogeneously dispersed at CNCs	397:467	A series of collagen/CNCs films were prepared by collagen solution with CNCs suspensions homogeneously dispersed at CNCs: collagen weight ratios of 1, 3, 5, 7, and 10.
23666851	7	13	theme	good	1054:1057	arg1	biocompatibility					1059:1074	good biocompatibility	1054:1074	good biocompatibility	1054:1074	In addition, in vitro cell culture studies revealed that the CNCs have no negative effect on the cell morphology, viability, and proliferation and possess good biocompatibility.
23666851	6	14	theme	%	817:817	arg1	loading					801:807	a loading	799:807	a loading of 7 wt %	799:817	Among the CNCs contents used, a loading of 7 wt % led to the maximum mechanical properties for the collagen/CNCs composite films.
23666851	3	15	theme	rod-like	255:262	arg1	CNCs					288:291	CNCs	288:291	CNCs	288:291	Here, rod-like cellulose nanocrystals (CNCs) were fabricated and used to reinforce collagen films.
23666851	3	15	theme	rod-like	255:262	arg1	nanocrystals					274:285	rod-like cellulose nanocrystals	255:285	rod-like cellulose nanocrystals (CNCs)	255:292	Here, rod-like cellulose nanocrystals (CNCs) were fabricated and used to reinforce collagen films.
23666851	4	16	theme	collagen/CNCs	360:372	arg1	films					374:378	collagen/CNCs films	360:378	collagen/CNCs films	360:378	A series of collagen/CNCs films were prepared by collagen solution with CNCs suspensions homogeneously dispersed at CNCs: collagen weight ratios of 1, 3, 5, 7, and 10.
23666851	1	17	theme	Collagen	90:97	arg1	films					99:103	Collagen films	90:103	Collagen films	90:103	Collagen films have been widely used in the field of biomedical engineering.
23666851	4	18	with	solution	406:413	arg1	suspensions					425:435	CNCs suspensions	420:435	CNCs suspensions homogeneously dispersed at CNCs	420:467	A series of collagen/CNCs films were prepared by collagen solution with CNCs suspensions homogeneously dispersed at CNCs: collagen weight ratios of 1, 3, 5, 7, and 10.
23666851	6	19	theme	wt	814:815	arg1	%					817:817	7 wt %	812:817	7 wt %	812:817	Among the CNCs contents used, a loading of 7 wt % led to the maximum mechanical properties for the collagen/CNCs composite films.
23666851	7	20	theme	cell	996:999	arg1	morphology					1001:1010	the cell morphology	992:1010	the cell morphology	992:1010	In addition, in vitro cell culture studies revealed that the CNCs have no negative effect on the cell morphology, viability, and proliferation and possess good biocompatibility.
23666851	7	21	contain	have	965:968	arg2	effect					982:987	no negative effect	970:987	no negative effect	970:987	In addition, in vitro cell culture studies revealed that the CNCs have no negative effect on the cell morphology, viability, and proliferation and possess good biocompatibility.
23666851	7	21	contain	have	965:968	arg1	CNCs					960:963	the CNCs	956:963	the CNCs	956:963	In addition, in vitro cell culture studies revealed that the CNCs have no negative effect on the cell morphology, viability, and proliferation and possess good biocompatibility.
23666851	8	22	theme	collagen	1163:1170	arg1	films					1172:1176	collagen films	1163:1176	collagen films	1163:1176	We conclude that the incorporation of CNCs is a simple and promising way to reinforce collagen films without impairing biocompatibility.
23666851	7	23	theme	negative	973:980	arg1	effect					982:987	no negative effect	970:987	no negative effect	970:987	In addition, in vitro cell culture studies revealed that the CNCs have no negative effect on the cell morphology, viability, and proliferation and possess good biocompatibility.
23666851	6	24	theme	CNCs	779:782	arg1	contents					784:791	the CNCs contents	775:791	the CNCs contents used	775:796	Among the CNCs contents used, a loading of 7 wt % led to the maximum mechanical properties for the collagen/CNCs composite films.
23666851	2	25	theme	collagen	210:217	arg1	properties					196:205	the poor mechanical properties	176:205	the poor mechanical properties of collagen	176:217	However, the poor mechanical properties of collagen have limited its application.
23666851	9	26	theme	good	1268:1271	arg1	potential					1273:1281	good potential	1268:1281	good potential for use	1268:1289	This study demonstrates that the composite films show good potential for use in the field of skin tissue engineering.
23666851	5	27	theme	properties	646:655	arg1	enhancement					610:620	the enhancement	606:620	the enhancement of the thermomechanical properties of the collagen/CNCs composites	606:687	The morphology of the resulting films was analyzed by scanning electron microscopy (SEM), the enhancement of the thermomechanical properties of the collagen/CNCs composites were demonstrated by thermal gravimetric analysis (TGA) and mechanical testing.
23666851	4	28	theme	films	374:378	arg1	series					350:355	A series	348:355	A series of collagen/CNCs films	348:378	A series of collagen/CNCs films were prepared by collagen solution with CNCs suspensions homogeneously dispersed at CNCs: collagen weight ratios of 1, 3, 5, 7, and 10.
23666851	8	29	theme	simple	1125:1130	arg1	way					1146:1148	a simple and promising way	1123:1148	a simple and promising way to reinforce collagen films without impairing biocompatibility	1123:1211	We conclude that the incorporation of CNCs is a simple and promising way to reinforce collagen films without impairing biocompatibility.
23666851	8	29	theme	simple	1125:1130	arg1	incorporation					1098:1110	the incorporation	1094:1110	the incorporation of CNCs	1094:1118	We conclude that the incorporation of CNCs is a simple and promising way to reinforce collagen films without impairing biocompatibility.
23666851	7	30	dep	in	912:913	arg1	vitro					915:919	vitro	915:919	vitro	915:919	In addition, in vitro cell culture studies revealed that the CNCs have no negative effect on the cell morphology, viability, and proliferation and possess good biocompatibility.
23666851	8	31	theme	CNCs	1115:1118	arg1	incorporation					1098:1110	the incorporation	1094:1110	the incorporation of CNCs	1094:1118	We conclude that the incorporation of CNCs is a simple and promising way to reinforce collagen films without impairing biocompatibility.
23666851	8	31	theme	CNCs	1115:1118	arg1	way					1146:1148	a simple and promising way	1123:1148	a simple and promising way to reinforce collagen films without impairing biocompatibility	1123:1211	We conclude that the incorporation of CNCs is a simple and promising way to reinforce collagen films without impairing biocompatibility.
23666851	2	32	theme	poor	180:183	arg1	properties					196:205	the poor mechanical properties	176:205	the poor mechanical properties of collagen	176:217	However, the poor mechanical properties of collagen have limited its application.
23666851	1	33	used	used	122:125	arg2	films					99:103	Collagen films	90:103	Collagen films	90:103	Collagen films have been widely used in the field of biomedical engineering.
23666851	0	34	theme	nanocrystals	40:51	arg1	properties					16:25	properties	16:25	properties	16:25	Preparation and properties of cellulose nanocrystals reinforced collagen composite films.
23666851	0	34	theme	nanocrystals	40:51	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and properties of cellulose nanocrystals reinforced collagen composite films.
23666851	3	35	theme	cellulose	264:272	arg1	CNCs					288:291	CNCs	288:291	CNCs	288:291	Here, rod-like cellulose nanocrystals (CNCs) were fabricated and used to reinforce collagen films.
23666851	3	35	theme	cellulose	264:272	arg1	nanocrystals					274:285	rod-like cellulose nanocrystals	255:285	rod-like cellulose nanocrystals (CNCs)	255:292	Here, rod-like cellulose nanocrystals (CNCs) were fabricated and used to reinforce collagen films.
23666851	0	36	theme	cellulose	30:38	arg1	nanocrystals					40:51	cellulose nanocrystals	30:51	cellulose nanocrystals	30:51	Preparation and properties of cellulose nanocrystals reinforced collagen composite films.
23666851	6	37	theme	composite	882:890	arg1	films					892:896	the collagen/CNCs composite films	864:896	the collagen/CNCs composite films	864:896	Among the CNCs contents used, a loading of 7 wt % led to the maximum mechanical properties for the collagen/CNCs composite films.
23666851	5	38	theme	collagen/CNCs	664:676	arg1	composites					678:687	the collagen/CNCs composites	660:687	the collagen/CNCs composites	660:687	The morphology of the resulting films was analyzed by scanning electron microscopy (SEM), the enhancement of the thermomechanical properties of the collagen/CNCs composites were demonstrated by thermal gravimetric analysis (TGA) and mechanical testing.
23666851	0	39	theme	collagen	64:71	arg1	films					83:87	collagen composite films	64:87	collagen composite films	64:87	Preparation and properties of cellulose nanocrystals reinforced collagen composite films.
23666851	6	40	theme	collagen/CNCs	868:880	arg1	films					892:896	the collagen/CNCs composite films	864:896	the collagen/CNCs composite films	864:896	Among the CNCs contents used, a loading of 7 wt % led to the maximum mechanical properties for the collagen/CNCs composite films.
23666851	0	41	theme	composite	73:81	arg1	films					83:87	collagen composite films	64:87	collagen composite films	64:87	Preparation and properties of cellulose nanocrystals reinforced collagen composite films.
23666851	9	42	theme	composite	1247:1255	arg1	films					1257:1261	the composite films	1243:1261	the composite films	1243:1261	This study demonstrates that the composite films show good potential for use in the field of skin tissue engineering.
23666851	5	43	theme	mechanical	749:758	arg1	testing					760:766	mechanical testing	749:766	mechanical testing	749:766	The morphology of the resulting films was analyzed by scanning electron microscopy (SEM), the enhancement of the thermomechanical properties of the collagen/CNCs composites were demonstrated by thermal gravimetric analysis (TGA) and mechanical testing.
23666851	4	44	theme	CNCs	420:423	arg1	suspensions					425:435	CNCs suspensions	420:435	CNCs suspensions homogeneously dispersed at CNCs	420:467	A series of collagen/CNCs films were prepared by collagen solution with CNCs suspensions homogeneously dispersed at CNCs: collagen weight ratios of 1, 3, 5, 7, and 10.
23666851	5	45	theme	electron	579:586	arg1	microscopy					588:597	scanning electron microscopy	570:597	scanning electron microscopy (SEM)	570:603	The morphology of the resulting films was analyzed by scanning electron microscopy (SEM), the enhancement of the thermomechanical properties of the collagen/CNCs composites were demonstrated by thermal gravimetric analysis (TGA) and mechanical testing.
23666851	5	45	theme	electron	579:586	arg1	SEM					600:602	SEM	600:602	SEM	600:602	The morphology of the resulting films was analyzed by scanning electron microscopy (SEM), the enhancement of the thermomechanical properties of the collagen/CNCs composites were demonstrated by thermal gravimetric analysis (TGA) and mechanical testing.
23666851	7	46	theme	culture	926:932	arg1	studies					934:940	in vitro cell culture studies	912:940	in vitro cell culture studies	912:940	In addition, in vitro cell culture studies revealed that the CNCs have no negative effect on the cell morphology, viability, and proliferation and possess good biocompatibility.
23666851	5	47	theme	resulting	538:546	arg1	films					548:552	the resulting films	534:552	the resulting films	534:552	The morphology of the resulting films was analyzed by scanning electron microscopy (SEM), the enhancement of the thermomechanical properties of the collagen/CNCs composites were demonstrated by thermal gravimetric analysis (TGA) and mechanical testing.
23666851	5	48	theme	gravimetric	718:728	arg1	TGA					740:742	TGA	740:742	TGA	740:742	The morphology of the resulting films was analyzed by scanning electron microscopy (SEM), the enhancement of the thermomechanical properties of the collagen/CNCs composites were demonstrated by thermal gravimetric analysis (TGA) and mechanical testing.
23666851	5	48	theme	gravimetric	718:728	arg1	analysis					730:737	thermal gravimetric analysis	710:737	thermal gravimetric analysis (TGA)	710:743	The morphology of the resulting films was analyzed by scanning electron microscopy (SEM), the enhancement of the thermomechanical properties of the collagen/CNCs composites were demonstrated by thermal gravimetric analysis (TGA) and mechanical testing.
23666851	5	49	theme	thermomechanical	629:644	arg1	properties					646:655	the thermomechanical properties	625:655	the thermomechanical properties of the collagen/CNCs composites	625:687	The morphology of the resulting films was analyzed by scanning electron microscopy (SEM), the enhancement of the thermomechanical properties of the collagen/CNCs composites were demonstrated by thermal gravimetric analysis (TGA) and mechanical testing.
23666851	8	50	theme	promising	1136:1144	arg1	way					1146:1148	a simple and promising way	1123:1148	a simple and promising way to reinforce collagen films without impairing biocompatibility	1123:1211	We conclude that the incorporation of CNCs is a simple and promising way to reinforce collagen films without impairing biocompatibility.
23666851	8	50	theme	promising	1136:1144	arg1	incorporation					1098:1110	the incorporation	1094:1110	the incorporation of CNCs	1094:1118	We conclude that the incorporation of CNCs is a simple and promising way to reinforce collagen films without impairing biocompatibility.
23666851	9	51	theme	skin	1307:1310	arg1	engineering					1319:1329	skin tissue engineering	1307:1329	skin tissue engineering	1307:1329	This study demonstrates that the composite films show good potential for use in the field of skin tissue engineering.
23666851	5	52	theme	thermal	710:716	arg1	TGA					740:742	TGA	740:742	TGA	740:742	The morphology of the resulting films was analyzed by scanning electron microscopy (SEM), the enhancement of the thermomechanical properties of the collagen/CNCs composites were demonstrated by thermal gravimetric analysis (TGA) and mechanical testing.
23666851	5	52	theme	thermal	710:716	arg1	analysis					730:737	thermal gravimetric analysis	710:737	thermal gravimetric analysis (TGA)	710:743	The morphology of the resulting films was analyzed by scanning electron microscopy (SEM), the enhancement of the thermomechanical properties of the collagen/CNCs composites were demonstrated by thermal gravimetric analysis (TGA) and mechanical testing.
23666851	2	53	theme	mechanical	185:194	arg1	properties					196:205	the poor mechanical properties	176:205	the poor mechanical properties of collagen	176:217	However, the poor mechanical properties of collagen have limited its application.
23666851	7	54	theme	cell	921:924	arg1	studies					934:940	in vitro cell culture studies	912:940	in vitro cell culture studies	912:940	In addition, in vitro cell culture studies revealed that the CNCs have no negative effect on the cell morphology, viability, and proliferation and possess good biocompatibility.
23666851	9	55	theme	tissue	1312:1317	arg1	engineering					1319:1329	skin tissue engineering	1307:1329	skin tissue engineering	1307:1329	This study demonstrates that the composite films show good potential for use in the field of skin tissue engineering.
23666851	3	56	theme	collagen	332:339	arg1	films					341:345	collagen films	332:345	collagen films	332:345	Here, rod-like cellulose nanocrystals (CNCs) were fabricated and used to reinforce collagen films.
23666851	1	57	theme	biomedical	143:152	arg1	engineering					154:164	biomedical engineering	143:164	biomedical engineering	143:164	Collagen films have been widely used in the field of biomedical engineering.
23666851	9	58	theme	engineering	1319:1329	arg1	field					1298:1302	the field	1294:1302	the field of skin tissue engineering	1294:1329	This study demonstrates that the composite films show good potential for use in the field of skin tissue engineering.
28887951	0	0	theme	system	112:117	arg1	engineering					126:136	central nervous system tissue engineering	96:136	central nervous system tissue engineering	96:136	Injectable hyaluronan-methylcellulose composite hydrogel crosslinked by polyethylene glycol for central nervous system tissue engineering.
28887951	4	1	theme	crosslinking	714:725	arg1	effects					727:733	crosslinking effects	714:733	the crosslinking effects polyethylene glycol brought to methylcellulose	710:780	Moreover, due to the crosslinking effects polyethylene glycol brought to methylcellulose, the rheological properties and stability of HAMC were greatly improved, which could prolong the residence time of this hydrogel effectively.
28887951	3	2	dep	forming	684:690	arg1	in-situ					676:682	in-situ forming	676:690	in-situ forming	676:690	HAMC combined the advantages of two components to be fast-gelling, injectable, degradable, biocompatible, and it was able to meet some special shape requirement for injured tissue by in-situ forming.
28887951	0	3	theme	nervous	104:110	arg1	system					112:117	central nervous system	96:117	central nervous system tissue engineering	96:136	Injectable hyaluronan-methylcellulose composite hydrogel crosslinked by polyethylene glycol for central nervous system tissue engineering.
28887951	5	4	theme	viability	929:937	arg1	results					939:945	Cell viability results	924:945	Cell viability results	924:945	Cell viability results showed HAMC was cytocompatible for further applications in vivo, and would be a promising choice for neural tissue engineering in the future.
28887951	5	5	dep	showed	947:952	arg1	choice					1037:1042	a promising choice	1025:1042	a promising choice for neural tissue engineering in the future	1025:1086	Cell viability results showed HAMC was cytocompatible for further applications in vivo, and would be a promising choice for neural tissue engineering in the future.
28887951	5	5	dep	showed	947:952	arg1	cytocompatible					963:976	cytocompatible	963:976	cytocompatible	963:976	Cell viability results showed HAMC was cytocompatible for further applications in vivo, and would be a promising choice for neural tissue engineering in the future.
28887951	0	6	theme	tissue	119:124	arg1	engineering					126:136	central nervous system tissue engineering	96:136	central nervous system tissue engineering	96:136	Injectable hyaluronan-methylcellulose composite hydrogel crosslinked by polyethylene glycol for central nervous system tissue engineering.
28887951	4	7	theme	hydrogel	902:909	arg1	time					889:892	the residence time	875:892	the residence time of this hydrogel	875:909	Moreover, due to the crosslinking effects polyethylene glycol brought to methylcellulose, the rheological properties and stability of HAMC were greatly improved, which could prolong the residence time of this hydrogel effectively.
28887951	4	8	theme	rheological	787:797	arg1	properties					799:808	rheological properties	787:808	rheological properties	787:808	Moreover, due to the crosslinking effects polyethylene glycol brought to methylcellulose, the rheological properties and stability of HAMC were greatly improved, which could prolong the residence time of this hydrogel effectively.
28887951	2	9	theme	hyaluronic	385:394	arg1	acid					396:399	hyaluronic acid	385:399	hyaluronic acid	385:399	To obtain a material with an appropriate degrading rate, an injectable composite hydrogel HAMC consisting of hyaluronic acid and methylcellulose was prepared using polyethylene glycol as a cross linker in this study.
28887951	2	10	theme	degrading	317:325	arg1	rate					327:330	an appropriate degrading rate	302:330	an appropriate degrading rate	302:330	To obtain a material with an appropriate degrading rate, an injectable composite hydrogel HAMC consisting of hyaluronic acid and methylcellulose was prepared using polyethylene glycol as a cross linker in this study.
28887951	5	11	from	choice	1037:1042	arg1	future					1081:1086	future	1081:1086	future	1081:1086	Cell viability results showed HAMC was cytocompatible for further applications in vivo, and would be a promising choice for neural tissue engineering in the future.
28887951	2	12	theme	appropriate	305:315	arg1	rate					327:330	an appropriate degrading rate	302:330	an appropriate degrading rate	302:330	To obtain a material with an appropriate degrading rate, an injectable composite hydrogel HAMC consisting of hyaluronic acid and methylcellulose was prepared using polyethylene glycol as a cross linker in this study.
28887951	3	13	theme	components	529:538	arg1	advantages					511:520	the advantages	507:520	the advantages of two components	507:538	HAMC combined the advantages of two components to be fast-gelling, injectable, degradable, biocompatible, and it was able to meet some special shape requirement for injured tissue by in-situ forming.
28887951	2	14	theme	polyethylene	440:451	arg1	glycol					453:458	polyethylene glycol	440:458	polyethylene glycol	440:458	To obtain a material with an appropriate degrading rate, an injectable composite hydrogel HAMC consisting of hyaluronic acid and methylcellulose was prepared using polyethylene glycol as a cross linker in this study.
28887951	2	14	theme	polyethylene	440:451	arg1	linker					471:476	a cross linker	463:476	a cross linker in this study	463:490	To obtain a material with an appropriate degrading rate, an injectable composite hydrogel HAMC consisting of hyaluronic acid and methylcellulose was prepared using polyethylene glycol as a cross linker in this study.
28887951	0	15	theme	hyaluronan-methylcellulose	11:36	arg1	hydrogel					48:55	Injectable hyaluronan-methylcellulose composite hydrogel	0:55	Injectable hyaluronan-methylcellulose composite hydrogel	0:55	Injectable hyaluronan-methylcellulose composite hydrogel crosslinked by polyethylene glycol for central nervous system tissue engineering.
28887951	2	16	theme	hydrogel	357:364	arg1	HAMC					366:369	an injectable composite hydrogel HAMC	333:369	an injectable composite hydrogel HAMC consisting of hyaluronic acid and methylcellulose	333:419	To obtain a material with an appropriate degrading rate, an injectable composite hydrogel HAMC consisting of hyaluronic acid and methylcellulose was prepared using polyethylene glycol as a cross linker in this study.
28887951	0	17	theme	Injectable	0:9	arg1	hydrogel					48:55	Injectable hyaluronan-methylcellulose composite hydrogel	0:55	Injectable hyaluronan-methylcellulose composite hydrogel	0:55	Injectable hyaluronan-methylcellulose composite hydrogel crosslinked by polyethylene glycol for central nervous system tissue engineering.
28887951	4	18	theme	residence	879:887	arg1	time					889:892	the residence time	875:892	the residence time of this hydrogel	875:909	Moreover, due to the crosslinking effects polyethylene glycol brought to methylcellulose, the rheological properties and stability of HAMC were greatly improved, which could prolong the residence time of this hydrogel effectively.
28887951	2	19	theme	composite	347:355	arg1	HAMC					366:369	an injectable composite hydrogel HAMC	333:369	an injectable composite hydrogel HAMC consisting of hyaluronic acid and methylcellulose	333:419	To obtain a material with an appropriate degrading rate, an injectable composite hydrogel HAMC consisting of hyaluronic acid and methylcellulose was prepared using polyethylene glycol as a cross linker in this study.
28887951	1	20	theme	tissue	223:228	arg1	techniques					242:251	tissue engineering techniques	223:251	tissue engineering techniques	223:251	Spontaneous recovery ability of central nerves has inspired researchers to focus on tissue engineering techniques, especially scaffolds.
28887951	5	21	theme	promising	1027:1035	arg1	choice					1037:1042	a promising choice	1025:1042	a promising choice for neural tissue engineering in the future	1025:1086	Cell viability results showed HAMC was cytocompatible for further applications in vivo, and would be a promising choice for neural tissue engineering in the future.
28887951	2	22	theme	injectable	336:345	arg1	HAMC					366:369	an injectable composite hydrogel HAMC	333:369	an injectable composite hydrogel HAMC consisting of hyaluronic acid and methylcellulose	333:419	To obtain a material with an appropriate degrading rate, an injectable composite hydrogel HAMC consisting of hyaluronic acid and methylcellulose was prepared using polyethylene glycol as a cross linker in this study.
28887951	1	23	theme	engineering	230:240	arg1	techniques					242:251	tissue engineering techniques	223:251	tissue engineering techniques	223:251	Spontaneous recovery ability of central nerves has inspired researchers to focus on tissue engineering techniques, especially scaffolds.
28887951	0	24	theme	composite	38:46	arg1	hydrogel					48:55	Injectable hyaluronan-methylcellulose composite hydrogel	0:55	Injectable hyaluronan-methylcellulose composite hydrogel	0:55	Injectable hyaluronan-methylcellulose composite hydrogel crosslinked by polyethylene glycol for central nervous system tissue engineering.
28887951	5	25	theme	further	982:988	arg1	applications					990:1001	further applications	982:1001	further applications	982:1001	Cell viability results showed HAMC was cytocompatible for further applications in vivo, and would be a promising choice for neural tissue engineering in the future.
28887951	1	26	theme	Spontaneous	139:149	arg1	ability					160:166	Spontaneous recovery ability	139:166	Spontaneous recovery ability of central nerves	139:184	Spontaneous recovery ability of central nerves has inspired researchers to focus on tissue engineering techniques, especially scaffolds.
28887951	2	27	theme	cross	465:469	arg1	glycol					453:458	polyethylene glycol	440:458	polyethylene glycol	440:458	To obtain a material with an appropriate degrading rate, an injectable composite hydrogel HAMC consisting of hyaluronic acid and methylcellulose was prepared using polyethylene glycol as a cross linker in this study.
28887951	2	27	theme	cross	465:469	arg1	linker					471:476	a cross linker	463:476	a cross linker in this study	463:490	To obtain a material with an appropriate degrading rate, an injectable composite hydrogel HAMC consisting of hyaluronic acid and methylcellulose was prepared using polyethylene glycol as a cross linker in this study.
28887951	4	28	theme	effects	727:733	arg1	glycol					748:753	the crosslinking effects polyethylene glycol	710:753	the crosslinking effects polyethylene glycol brought to methylcellulose	710:780	Moreover, due to the crosslinking effects polyethylene glycol brought to methylcellulose, the rheological properties and stability of HAMC were greatly improved, which could prolong the residence time of this hydrogel effectively.
28887951	4	29	dep	properties	799:808	arg1	the					783:785	the	783:785	the	783:785	Moreover, due to the crosslinking effects polyethylene glycol brought to methylcellulose, the rheological properties and stability of HAMC were greatly improved, which could prolong the residence time of this hydrogel effectively.
28887951	2	30	with	material	288:295	arg1	rate					327:330	an appropriate degrading rate	302:330	an appropriate degrading rate	302:330	To obtain a material with an appropriate degrading rate, an injectable composite hydrogel HAMC consisting of hyaluronic acid and methylcellulose was prepared using polyethylene glycol as a cross linker in this study.
28887951	3	31	theme	injured	658:664	arg1	tissue					666:671	injured tissue	658:671	injured tissue	658:671	HAMC combined the advantages of two components to be fast-gelling, injectable, degradable, biocompatible, and it was able to meet some special shape requirement for injured tissue by in-situ forming.
28887951	5	32	theme	neural	1048:1053	arg1	engineering					1062:1072	neural tissue engineering	1048:1072	neural tissue engineering	1048:1072	Cell viability results showed HAMC was cytocompatible for further applications in vivo, and would be a promising choice for neural tissue engineering in the future.
28887951	5	33	theme	tissue	1055:1060	arg1	engineering					1062:1072	neural tissue engineering	1048:1072	neural tissue engineering	1048:1072	Cell viability results showed HAMC was cytocompatible for further applications in vivo, and would be a promising choice for neural tissue engineering in the future.
28887951	1	34	theme	recovery	151:158	arg1	ability					160:166	Spontaneous recovery ability	139:166	Spontaneous recovery ability of central nerves	139:184	Spontaneous recovery ability of central nerves has inspired researchers to focus on tissue engineering techniques, especially scaffolds.
28887951	0	35	theme	polyethylene	72:83	arg1	glycol					85:90	polyethylene glycol	72:90	polyethylene glycol for central nervous system tissue engineering	72:136	Injectable hyaluronan-methylcellulose composite hydrogel crosslinked by polyethylene glycol for central nervous system tissue engineering.
28887951	3	36	dep	fast-gelling	546:557	arg1	biocompatible					584:596	biocompatible	584:596	biocompatible	584:596	HAMC combined the advantages of two components to be fast-gelling, injectable, degradable, biocompatible, and it was able to meet some special shape requirement for injured tissue by in-situ forming.
28887951	3	36	dep	fast-gelling	546:557	arg1	degradable					572:581	degradable	572:581	degradable	572:581	HAMC combined the advantages of two components to be fast-gelling, injectable, degradable, biocompatible, and it was able to meet some special shape requirement for injured tissue by in-situ forming.
28887951	3	36	dep	fast-gelling	546:557	arg1	injectable					560:569	injectable	560:569	injectable	560:569	HAMC combined the advantages of two components to be fast-gelling, injectable, degradable, biocompatible, and it was able to meet some special shape requirement for injured tissue by in-situ forming.
28887951	4	37	theme	polyethylene	735:746	arg1	glycol					748:753	the crosslinking effects polyethylene glycol	710:753	the crosslinking effects polyethylene glycol brought to methylcellulose	710:780	Moreover, due to the crosslinking effects polyethylene glycol brought to methylcellulose, the rheological properties and stability of HAMC were greatly improved, which could prolong the residence time of this hydrogel effectively.
28887951	4	38	theme	HAMC	827:830	arg1	properties					799:808	rheological properties	787:808	rheological properties	787:808	Moreover, due to the crosslinking effects polyethylene glycol brought to methylcellulose, the rheological properties and stability of HAMC were greatly improved, which could prolong the residence time of this hydrogel effectively.
28887951	4	38	theme	HAMC	827:830	arg1	stability					814:822	stability	814:822	stability	814:822	Moreover, due to the crosslinking effects polyethylene glycol brought to methylcellulose, the rheological properties and stability of HAMC were greatly improved, which could prolong the residence time of this hydrogel effectively.
28887951	1	39	theme	central	171:177	arg1	nerves					179:184	central nerves	171:184	central nerves	171:184	Spontaneous recovery ability of central nerves has inspired researchers to focus on tissue engineering techniques, especially scaffolds.
28887951	0	40	theme	central	96:102	arg1	system					112:117	central nervous system	96:117	central nervous system tissue engineering	96:136	Injectable hyaluronan-methylcellulose composite hydrogel crosslinked by polyethylene glycol for central nervous system tissue engineering.
28887951	3	41	theme	special	628:634	arg1	requirement					642:652	some special shape requirement	623:652	some special shape requirement for injured tissue by in-situ forming	623:690	HAMC combined the advantages of two components to be fast-gelling, injectable, degradable, biocompatible, and it was able to meet some special shape requirement for injured tissue by in-situ forming.
28887951	1	42	theme	nerves	179:184	arg1	ability					160:166	Spontaneous recovery ability	139:166	Spontaneous recovery ability of central nerves	139:184	Spontaneous recovery ability of central nerves has inspired researchers to focus on tissue engineering techniques, especially scaffolds.
28887951	3	43	theme	shape	636:640	arg1	requirement					642:652	some special shape requirement	623:652	some special shape requirement for injured tissue by in-situ forming	623:690	HAMC combined the advantages of two components to be fast-gelling, injectable, degradable, biocompatible, and it was able to meet some special shape requirement for injured tissue by in-situ forming.
28887951	2	44	from	linker	471:476	arg1	study					486:490	this study	481:490	this study	481:490	To obtain a material with an appropriate degrading rate, an injectable composite hydrogel HAMC consisting of hyaluronic acid and methylcellulose was prepared using polyethylene glycol as a cross linker in this study.
28887951	5	45	theme	Cell	924:927	arg1	results					939:945	Cell viability results	924:945	Cell viability results	924:945	Cell viability results showed HAMC was cytocompatible for further applications in vivo, and would be a promising choice for neural tissue engineering in the future.
27474637	6	0	from	inclusion	1056:1064	arg1	RBC					1075:1077	RBC	1075:1077	RBC	1075:1077	Changes in the hydrophilic characteristics were also observed and owing to the adhesion effect the inclusion of HD on RBC led to a statistically significant effect of the mechanical properties of films.
27474637	1	1	theme	residual	187:194	arg1	recycled					267:274	recycled	267:274	mechanically defibrillated (RBC fibrils) recycled following wound dressing production via a dry-cast process	226:333	Reconstituted cellulose films were generated using residual bacterial cellulose membranes mechanically defibrillated (RBC fibrils) recycled following wound dressing production via a dry-cast process.
27474637	1	1	theme	residual	187:194	arg1	fibrils					258:264	RBC fibrils	254:264	RBC fibrils	254:264	Reconstituted cellulose films were generated using residual bacterial cellulose membranes mechanically defibrillated (RBC fibrils) recycled following wound dressing production via a dry-cast process.
27474637	1	1	theme	residual	187:194	arg1	membranes					216:224	residual bacterial cellulose membranes	187:224	residual bacterial cellulose membranes mechanically defibrillated (RBC fibrils) recycled following wound dressing production via a dry-cast process	187:333	Reconstituted cellulose films were generated using residual bacterial cellulose membranes mechanically defibrillated (RBC fibrils) recycled following wound dressing production via a dry-cast process.
27474637	5	2	theme	RBC	809:811	arg1	films					813:817	RBC films	809:817	RBC films with 20-30% GHXG replacement	809:846	RBC films with 20-30% GHXG replacement improved film stability and the inclusion of HD increased microfiber aggregation and reduced porous regions.
27474637	1	3	theme	bacterial	196:204	arg1	recycled					267:274	recycled	267:274	mechanically defibrillated (RBC fibrils) recycled following wound dressing production via a dry-cast process	226:333	Reconstituted cellulose films were generated using residual bacterial cellulose membranes mechanically defibrillated (RBC fibrils) recycled following wound dressing production via a dry-cast process.
27474637	1	3	theme	bacterial	196:204	arg1	fibrils					258:264	RBC fibrils	254:264	RBC fibrils	254:264	Reconstituted cellulose films were generated using residual bacterial cellulose membranes mechanically defibrillated (RBC fibrils) recycled following wound dressing production via a dry-cast process.
27474637	1	3	theme	bacterial	196:204	arg1	membranes					216:224	residual bacterial cellulose membranes	187:224	residual bacterial cellulose membranes mechanically defibrillated (RBC fibrils) recycled following wound dressing production via a dry-cast process	187:333	Reconstituted cellulose films were generated using residual bacterial cellulose membranes mechanically defibrillated (RBC fibrils) recycled following wound dressing production via a dry-cast process.
27474637	6	4	theme	adhesion	1036:1043	arg1	effect					1045:1050	the adhesion effect	1032:1050	the adhesion effect	1032:1050	Changes in the hydrophilic characteristics were also observed and owing to the adhesion effect the inclusion of HD on RBC led to a statistically significant effect of the mechanical properties of films.
27474637	7	5	theme	commercial	1230:1239	arg1	cellulose					1251:1259	commercial bacterial cellulose	1230:1259	commercial bacterial cellulose	1230:1259	The RBC/AG films supported L929 adhesion similar to that observed for commercial bacterial cellulose, indicating their potential use for biomedical applications.
27474637	3	6	theme	electron	617:624	arg1	microscopy					626:635	scanning electron microscopy	608:635	scanning electron microscopy	608:635	Biocomposite properties were evaluated by scanning electron microscopy, contact angle (CA), and X-ray diffraction measurements.
27474637	4	7	theme	fibroblasts	742:752	arg1	attachment					698:707	attachment	698:707	attachment	698:707	The attachment and proliferation of murine L929 fibroblasts on RBC and RBC/Hydrocolloids (HD) were also evaluated.
27474637	4	7	theme	fibroblasts	742:752	arg1	proliferation					713:725	proliferation	713:725	proliferation	713:725	The attachment and proliferation of murine L929 fibroblasts on RBC and RBC/Hydrocolloids (HD) were also evaluated.
27474637	4	8	from	proliferation	713:725	arg1	RBC					757:759	RBC	757:759	RBC	757:759	The attachment and proliferation of murine L929 fibroblasts on RBC and RBC/Hydrocolloids (HD) were also evaluated.
27474637	4	8	from	proliferation	713:725	arg1	HD					784:785	HD	784:785	HD	784:785	The attachment and proliferation of murine L929 fibroblasts on RBC and RBC/Hydrocolloids (HD) were also evaluated.
27474637	4	8	from	proliferation	713:725	arg1	RBC/Hydrocolloids					765:781	RBC/Hydrocolloids	765:781	RBC/Hydrocolloids (HD)	765:786	The attachment and proliferation of murine L929 fibroblasts on RBC and RBC/Hydrocolloids (HD) were also evaluated.
27474637	1	9	theme	dry-cast	318:325	arg1	process					327:333	a dry-cast process	316:333	a dry-cast process	316:333	Reconstituted cellulose films were generated using residual bacterial cellulose membranes mechanically defibrillated (RBC fibrils) recycled following wound dressing production via a dry-cast process.
27474637	2	10	theme	same	552:555	arg1	process					557:563	the same process	548:563	the same process	548:563	Arabinogalactan (AG) extracted from Pereskia aculeata leaves and/or a xyloglucan (GHXG) from Guibourtia hymenifolia seeds were incorporating into the RBC at various compositions, and new films were created using the same process.
27474637	2	11	from	compositions	501:512	arg1	RBC					486:488	the RBC	482:488	the RBC at various compositions	482:512	Arabinogalactan (AG) extracted from Pereskia aculeata leaves and/or a xyloglucan (GHXG) from Guibourtia hymenifolia seeds were incorporating into the RBC at various compositions, and new films were created using the same process.
27474637	7	12	theme	similar	1201:1207	arg1	adhesion					1192:1199	L929 adhesion	1187:1199	L929 adhesion similar to that observed for commercial bacterial cellulose	1187:1259	The RBC/AG films supported L929 adhesion similar to that observed for commercial bacterial cellulose, indicating their potential use for biomedical applications.
27474637	3	13	theme	Biocomposite	566:577	arg1	properties					579:588	Biocomposite properties	566:588	Biocomposite properties	566:588	Biocomposite properties were evaluated by scanning electron microscopy, contact angle (CA), and X-ray diffraction measurements.
27474637	1	14	theme	cellulose	206:214	arg1	recycled					267:274	recycled	267:274	mechanically defibrillated (RBC fibrils) recycled following wound dressing production via a dry-cast process	226:333	Reconstituted cellulose films were generated using residual bacterial cellulose membranes mechanically defibrillated (RBC fibrils) recycled following wound dressing production via a dry-cast process.
27474637	1	14	theme	cellulose	206:214	arg1	fibrils					258:264	RBC fibrils	254:264	RBC fibrils	254:264	Reconstituted cellulose films were generated using residual bacterial cellulose membranes mechanically defibrillated (RBC fibrils) recycled following wound dressing production via a dry-cast process.
27474637	1	14	theme	cellulose	206:214	arg1	membranes					216:224	residual bacterial cellulose membranes	187:224	residual bacterial cellulose membranes mechanically defibrillated (RBC fibrils) recycled following wound dressing production via a dry-cast process	187:333	Reconstituted cellulose films were generated using residual bacterial cellulose membranes mechanically defibrillated (RBC fibrils) recycled following wound dressing production via a dry-cast process.
27474637	5	15	theme	%	829:829	arg1	replacement					836:846	20-30% GHXG replacement	824:846	20-30% GHXG replacement	824:846	RBC films with 20-30% GHXG replacement improved film stability and the inclusion of HD increased microfiber aggregation and reduced porous regions.
27474637	5	16	theme	porous	941:946	arg1	regions					948:954	porous regions	941:954	porous regions	941:954	RBC films with 20-30% GHXG replacement improved film stability and the inclusion of HD increased microfiber aggregation and reduced porous regions.
27474637	4	17	from	attachment	698:707	arg1	RBC					757:759	RBC	757:759	RBC	757:759	The attachment and proliferation of murine L929 fibroblasts on RBC and RBC/Hydrocolloids (HD) were also evaluated.
27474637	4	17	from	attachment	698:707	arg1	HD					784:785	HD	784:785	HD	784:785	The attachment and proliferation of murine L929 fibroblasts on RBC and RBC/Hydrocolloids (HD) were also evaluated.
27474637	4	17	from	attachment	698:707	arg1	RBC/Hydrocolloids					765:781	RBC/Hydrocolloids	765:781	RBC/Hydrocolloids (HD)	765:786	The attachment and proliferation of murine L929 fibroblasts on RBC and RBC/Hydrocolloids (HD) were also evaluated.
27474637	2	18	theme	Guibourtia	429:438	arg1	seeds					452:456	Guibourtia hymenifolia seeds	429:456	Guibourtia hymenifolia seeds	429:456	Arabinogalactan (AG) extracted from Pereskia aculeata leaves and/or a xyloglucan (GHXG) from Guibourtia hymenifolia seeds were incorporating into the RBC at various compositions, and new films were created using the same process.
27474637	2	19	theme	various	493:499	arg1	compositions					501:512	various compositions	493:512	various compositions	493:512	Arabinogalactan (AG) extracted from Pereskia aculeata leaves and/or a xyloglucan (GHXG) from Guibourtia hymenifolia seeds were incorporating into the RBC at various compositions, and new films were created using the same process.
27474637	6	20	from	Changes	957:963	arg1	characteristics					984:998	the hydrophilic characteristics	968:998	the hydrophilic characteristics	968:998	Changes in the hydrophilic characteristics were also observed and owing to the adhesion effect the inclusion of HD on RBC led to a statistically significant effect of the mechanical properties of films.
27474637	2	21	dep	aculeata	381:388	arg1	leaves					390:395	leaves	390:395	leaves	390:395	Arabinogalactan (AG) extracted from Pereskia aculeata leaves and/or a xyloglucan (GHXG) from Guibourtia hymenifolia seeds were incorporating into the RBC at various compositions, and new films were created using the same process.
27474637	4	22	theme	L929	737:740	arg1	fibroblasts					742:752	murine L929 fibroblasts	730:752	murine L929 fibroblasts	730:752	The attachment and proliferation of murine L929 fibroblasts on RBC and RBC/Hydrocolloids (HD) were also evaluated.
27474637	6	23	theme	significant	1102:1112	arg1	effect					1114:1119	a statistically significant effect	1086:1119	a statistically significant effect of the mechanical properties of films	1086:1157	Changes in the hydrophilic characteristics were also observed and owing to the adhesion effect the inclusion of HD on RBC led to a statistically significant effect of the mechanical properties of films.
27474637	0	24	theme	in	20:21	arg1	biocompatibility					29:44	in vitro biocompatibility	20:44	in vitro biocompatibility	20:44	Physicochemical and in vitro biocompatibility of films combining reconstituted bacterial cellulose with arabinogalactan and xyloglucan.
27474637	5	25	with	films	813:817	arg1	replacement					836:846	20-30% GHXG replacement	824:846	20-30% GHXG replacement	824:846	RBC films with 20-30% GHXG replacement improved film stability and the inclusion of HD increased microfiber aggregation and reduced porous regions.
27474637	3	26	theme	scanning	608:615	arg1	microscopy					626:635	scanning electron microscopy	608:635	scanning electron microscopy	608:635	Biocomposite properties were evaluated by scanning electron microscopy, contact angle (CA), and X-ray diffraction measurements.
27474637	3	27	dep	microscopy	626:635	arg1	measurements					680:691	measurements	680:691	measurements	680:691	Biocomposite properties were evaluated by scanning electron microscopy, contact angle (CA), and X-ray diffraction measurements.
27474637	1	28	theme	RBC	254:256	arg1	fibrils					258:264	RBC fibrils	254:264	RBC fibrils	254:264	Reconstituted cellulose films were generated using residual bacterial cellulose membranes mechanically defibrillated (RBC fibrils) recycled following wound dressing production via a dry-cast process.
27474637	1	28	theme	RBC	254:256	arg1	membranes					216:224	residual bacterial cellulose membranes	187:224	residual bacterial cellulose membranes mechanically defibrillated (RBC fibrils) recycled following wound dressing production via a dry-cast process	187:333	Reconstituted cellulose films were generated using residual bacterial cellulose membranes mechanically defibrillated (RBC fibrils) recycled following wound dressing production via a dry-cast process.
27474637	2	29	theme	new	519:521	arg1	films					523:527	new films	519:527	new films	519:527	Arabinogalactan (AG) extracted from Pereskia aculeata leaves and/or a xyloglucan (GHXG) from Guibourtia hymenifolia seeds were incorporating into the RBC at various compositions, and new films were created using the same process.
27474637	4	30	attach	attachment	698:707	arg3	RBC/Hydrocolloids					765:781	RBC/Hydrocolloids	765:781	RBC/Hydrocolloids (HD)	765:786	The attachment and proliferation of murine L929 fibroblasts on RBC and RBC/Hydrocolloids (HD) were also evaluated.
27474637	4	30	attach	attachment	698:707	arg3	HD					784:785	HD	784:785	HD	784:785	The attachment and proliferation of murine L929 fibroblasts on RBC and RBC/Hydrocolloids (HD) were also evaluated.
27474637	4	30	attach	attachment	698:707	arg2	fibroblasts					742:752	murine L929 fibroblasts	730:752	murine L929 fibroblasts	730:752	The attachment and proliferation of murine L929 fibroblasts on RBC and RBC/Hydrocolloids (HD) were also evaluated.
27474637	4	30	attach	attachment	698:707	arg3	RBC					757:759	RBC	757:759	RBC	757:759	The attachment and proliferation of murine L929 fibroblasts on RBC and RBC/Hydrocolloids (HD) were also evaluated.
27474637	5	31	theme	microfiber	906:915	arg1	aggregation					917:927	microfiber aggregation	906:927	microfiber aggregation	906:927	RBC films with 20-30% GHXG replacement improved film stability and the inclusion of HD increased microfiber aggregation and reduced porous regions.
27474637	7	32	theme	RBC/AG	1164:1169	arg1	films					1171:1175	The RBC/AG films	1160:1175	The RBC/AG films	1160:1175	The RBC/AG films supported L929 adhesion similar to that observed for commercial bacterial cellulose, indicating their potential use for biomedical applications.
27474637	2	33	theme	hymenifolia	440:450	arg1	seeds					452:456	Guibourtia hymenifolia seeds	429:456	Guibourtia hymenifolia seeds	429:456	Arabinogalactan (AG) extracted from Pereskia aculeata leaves and/or a xyloglucan (GHXG) from Guibourtia hymenifolia seeds were incorporating into the RBC at various compositions, and new films were created using the same process.
27474637	7	34	theme	L929	1187:1190	arg1	adhesion					1192:1199	L929 adhesion	1187:1199	L929 adhesion similar to that observed for commercial bacterial cellulose	1187:1259	The RBC/AG films supported L929 adhesion similar to that observed for commercial bacterial cellulose, indicating their potential use for biomedical applications.
27474637	3	35	theme	X-ray	662:666	arg1	diffraction					668:678	X-ray diffraction	662:678	X-ray diffraction	662:678	Biocomposite properties were evaluated by scanning electron microscopy, contact angle (CA), and X-ray diffraction measurements.
27474637	1	36	dep	defibrillated	239:251	arg1	recycled					267:274	recycled	267:274	mechanically defibrillated (RBC fibrils) recycled following wound dressing production via a dry-cast process	226:333	Reconstituted cellulose films were generated using residual bacterial cellulose membranes mechanically defibrillated (RBC fibrils) recycled following wound dressing production via a dry-cast process.
27474637	1	36	dep	defibrillated	239:251	arg1	fibrils					258:264	RBC fibrils	254:264	RBC fibrils	254:264	Reconstituted cellulose films were generated using residual bacterial cellulose membranes mechanically defibrillated (RBC fibrils) recycled following wound dressing production via a dry-cast process.
27474637	1	36	dep	defibrillated	239:251	arg1	membranes					216:224	residual bacterial cellulose membranes	187:224	residual bacterial cellulose membranes mechanically defibrillated (RBC fibrils) recycled following wound dressing production via a dry-cast process	187:333	Reconstituted cellulose films were generated using residual bacterial cellulose membranes mechanically defibrillated (RBC fibrils) recycled following wound dressing production via a dry-cast process.
27474637	5	37	theme	20-30	824:828	arg1	%					829:829	%	829:829	%	829:829	RBC films with 20-30% GHXG replacement improved film stability and the inclusion of HD increased microfiber aggregation and reduced porous regions.
27474637	6	38	theme	properties	1139:1148	arg1	effect					1114:1119	a statistically significant effect	1086:1119	a statistically significant effect of the mechanical properties of films	1086:1157	Changes in the hydrophilic characteristics were also observed and owing to the adhesion effect the inclusion of HD on RBC led to a statistically significant effect of the mechanical properties of films.
27474637	1	39	theme	Reconstituted	136:148	arg1	films					160:164	Reconstituted cellulose films	136:164	Reconstituted cellulose films	136:164	Reconstituted cellulose films were generated using residual bacterial cellulose membranes mechanically defibrillated (RBC fibrils) recycled following wound dressing production via a dry-cast process.
27474637	0	40	theme	films	49:53	arg1	Physicochemical					0:14	Physicochemical	0:14	Physicochemical	0:14	Physicochemical and in vitro biocompatibility of films combining reconstituted bacterial cellulose with arabinogalactan and xyloglucan.
27474637	0	40	theme	films	49:53	arg1	biocompatibility					29:44	in vitro biocompatibility	20:44	in vitro biocompatibility	20:44	Physicochemical and in vitro biocompatibility of films combining reconstituted bacterial cellulose with arabinogalactan and xyloglucan.
27474637	6	41	theme	hydrophilic	972:982	arg1	characteristics					984:998	the hydrophilic characteristics	968:998	the hydrophilic characteristics	968:998	Changes in the hydrophilic characteristics were also observed and owing to the adhesion effect the inclusion of HD on RBC led to a statistically significant effect of the mechanical properties of films.
27474637	3	42	theme	contact	638:644	arg1	angle					646:650	contact angle	638:650	contact angle (CA)	638:655	Biocomposite properties were evaluated by scanning electron microscopy, contact angle (CA), and X-ray diffraction measurements.
27474637	3	42	theme	contact	638:644	arg1	CA					653:654	CA	653:654	CA	653:654	Biocomposite properties were evaluated by scanning electron microscopy, contact angle (CA), and X-ray diffraction measurements.
27474637	7	43	theme	bacterial	1241:1249	arg1	cellulose					1251:1259	commercial bacterial cellulose	1230:1259	commercial bacterial cellulose	1230:1259	The RBC/AG films supported L929 adhesion similar to that observed for commercial bacterial cellulose, indicating their potential use for biomedical applications.
27474637	1	44	theme	cellulose	150:158	arg1	films					160:164	Reconstituted cellulose films	136:164	Reconstituted cellulose films	136:164	Reconstituted cellulose films were generated using residual bacterial cellulose membranes mechanically defibrillated (RBC fibrils) recycled following wound dressing production via a dry-cast process.
27474637	7	45	theme	biomedical	1297:1306	arg1	applications					1308:1319	biomedical applications	1297:1319	biomedical applications	1297:1319	The RBC/AG films supported L929 adhesion similar to that observed for commercial bacterial cellulose, indicating their potential use for biomedical applications.
27474637	6	46	theme	films	1153:1157	arg1	properties					1139:1148	the mechanical properties	1124:1148	the mechanical properties of films	1124:1157	Changes in the hydrophilic characteristics were also observed and owing to the adhesion effect the inclusion of HD on RBC led to a statistically significant effect of the mechanical properties of films.
27474637	6	47	theme	HD	1069:1070	arg1	inclusion					1056:1064	the inclusion	1052:1064	the inclusion of HD on RBC	1052:1077	Changes in the hydrophilic characteristics were also observed and owing to the adhesion effect the inclusion of HD on RBC led to a statistically significant effect of the mechanical properties of films.
27474637	0	48	dep	in	20:21	arg1	vitro					23:27	vitro	23:27	vitro	23:27	Physicochemical and in vitro biocompatibility of films combining reconstituted bacterial cellulose with arabinogalactan and xyloglucan.
27474637	2	49	theme	Pereskia	372:379	arg1	aculeata					381:388	Pereskia aculeata	372:388	Pereskia aculeata leaves	372:395	Arabinogalactan (AG) extracted from Pereskia aculeata leaves and/or a xyloglucan (GHXG) from Guibourtia hymenifolia seeds were incorporating into the RBC at various compositions, and new films were created using the same process.
27474637	0	50	theme	bacterial	79:87	arg1	cellulose					89:97	reconstituted bacterial cellulose	65:97	reconstituted bacterial cellulose	65:97	Physicochemical and in vitro biocompatibility of films combining reconstituted bacterial cellulose with arabinogalactan and xyloglucan.
27474637	6	51	theme	mechanical	1128:1137	arg1	properties					1139:1148	the mechanical properties	1124:1148	the mechanical properties of films	1124:1157	Changes in the hydrophilic characteristics were also observed and owing to the adhesion effect the inclusion of HD on RBC led to a statistically significant effect of the mechanical properties of films.
27474637	4	52	dep	attachment	698:707	arg1	The					694:696	The	694:696	The	694:696	The attachment and proliferation of murine L929 fibroblasts on RBC and RBC/Hydrocolloids (HD) were also evaluated.
27474637	4	53	theme	murine	730:735	arg1	fibroblasts					742:752	murine L929 fibroblasts	730:752	murine L929 fibroblasts	730:752	The attachment and proliferation of murine L929 fibroblasts on RBC and RBC/Hydrocolloids (HD) were also evaluated.
27474637	5	54	theme	HD	893:894	arg1	inclusion					880:888	the inclusion	876:888	the inclusion of HD	876:894	RBC films with 20-30% GHXG replacement improved film stability and the inclusion of HD increased microfiber aggregation and reduced porous regions.
27474637	1	55	theme	wound	286:290	arg1	production					301:310	wound dressing production	286:310	wound dressing production	286:310	Reconstituted cellulose films were generated using residual bacterial cellulose membranes mechanically defibrillated (RBC fibrils) recycled following wound dressing production via a dry-cast process.
27474637	0	56	theme	reconstituted	65:77	arg1	cellulose					89:97	reconstituted bacterial cellulose	65:97	reconstituted bacterial cellulose	65:97	Physicochemical and in vitro biocompatibility of films combining reconstituted bacterial cellulose with arabinogalactan and xyloglucan.
27474637	5	57	theme	film	857:860	arg1	stability					862:870	film stability	857:870	film stability	857:870	RBC films with 20-30% GHXG replacement improved film stability and the inclusion of HD increased microfiber aggregation and reduced porous regions.
27474637	5	58	theme	GHXG	831:834	arg1	replacement					836:846	20-30% GHXG replacement	824:846	20-30% GHXG replacement	824:846	RBC films with 20-30% GHXG replacement improved film stability and the inclusion of HD increased microfiber aggregation and reduced porous regions.
27474637	7	59	theme	potential	1279:1287	arg1	use					1289:1291	their potential use	1273:1291	their potential use for biomedical applications	1273:1319	The RBC/AG films supported L929 adhesion similar to that observed for commercial bacterial cellulose, indicating their potential use for biomedical applications.
27474637	1	60	theme	dressing	292:299	arg1	production					301:310	wound dressing production	286:310	wound dressing production	286:310	Reconstituted cellulose films were generated using residual bacterial cellulose membranes mechanically defibrillated (RBC fibrils) recycled following wound dressing production via a dry-cast process.
26230333	2	0	theme	biofilm	449:455	arg1	composition					420:430	composition	420:430	composition	420:430	The aim of this study was to determine how the twice-daily treatment with blue light affects the development and composition of a matrix-rich biofilm.
26230333	2	0	theme	biofilm	449:455	arg1	development					404:414	development	404:414	development	404:414	The aim of this study was to determine how the twice-daily treatment with blue light affects the development and composition of a matrix-rich biofilm.
26230333	7	1	theme	laser	1134:1138	arg1	microscopy					1140:1149	confocal scanning laser microscopy	1116:1149	confocal scanning laser microscopy	1116:1149	Variable pressure scanning electron microscopy and confocal scanning laser microscopy were used to check biofilm morphology and bacterial viability, respectively.
26230333	1	2	theme	direct	135:140	arg1	use					94:96	The use	90:96	The use of blue light	90:110	BACKGROUND The use of blue light has been proposed as a direct means of affecting local bacterial infections, however the use of blue light without a photosensitizer to prevent the biofilm development has not yet been explored.
26230333	1	2	theme	direct	135:140	arg1	means					142:146	a direct means	133:146	a direct means of affecting local bacterial infections, however the use of blue light without a photosensitizer to prevent the biofilm development has not yet been explored	133:304	BACKGROUND The use of blue light has been proposed as a direct means of affecting local bacterial infections, however the use of blue light without a photosensitizer to prevent the biofilm development has not yet been explored.
26230333	8	3	theme	0.89	1365:1368	arg1	%					1369:1369	%	1369:1369	%	1369:1369	When biofilms were exposed to twice-daily blue light, EPS-insoluble was reduced significantly more than in either control group (CHX and 0.89% NaCl).
26230333	2	4	theme	matrix-rich	437:447	arg1	biofilm					449:455	a matrix-rich biofilm	435:455	a matrix-rich biofilm	435:455	The aim of this study was to determine how the twice-daily treatment with blue light affects the development and composition of a matrix-rich biofilm.
26230333	5	5	theme	Positive	822:829	arg1	controls					844:851	56 s. Positive and negative controls	816:851	56 s. Positive and negative controls	816:851	The distance between the light and the sample was 1.0 cm; energy density of 72 J cm-2; and exposure time of 12 min 56 s. Positive and negative controls were twice-daily 0.12% chlorhexidine (CHX) and 0.89% NaCl, respectively.
26230333	5	6	dep	%	874:874	arg1	0.12					870:873	0.12	870:873	0.12	870:873	The distance between the light and the sample was 1.0 cm; energy density of 72 J cm-2; and exposure time of 12 min 56 s. Positive and negative controls were twice-daily 0.12% chlorhexidine (CHX) and 0.89% NaCl, respectively.
26230333	9	7	theme	dry	1402:1404	arg1	weight					1406:1411	dry weight	1402:1411	dry weight	1402:1411	Bacterial viability and dry weight were also reduced relative to the negative control (0.89% NaCl) when the biofilms were treated with twice-daily blue light.
26230333	10	8	theme	blue	1611:1614	arg1	light					1616:1620	blue light	1611:1620	blue light	1611:1620	Different morphology was also visible when the biofilms were treated with blue light.
26230333	7	9	theme	confocal	1116:1123	arg1	microscopy					1140:1149	confocal scanning laser microscopy	1116:1149	confocal scanning laser microscopy	1116:1149	Variable pressure scanning electron microscopy and confocal scanning laser microscopy were used to check biofilm morphology and bacterial viability, respectively.
26230333	0	10	from	Effect	0:5	arg1	Development					66:76	Matrix-Rich Biofilm Development	46:76	Matrix-Rich Biofilm Development	46:76	Effect of Twice-Daily Blue Light Treatment on Matrix-Rich Biofilm Development.
26230333	5	11	dep	density	766:772	arg1	controls					844:851	56 s. Positive and negative controls	816:851	56 s. Positive and negative controls	816:851	The distance between the light and the sample was 1.0 cm; energy density of 72 J cm-2; and exposure time of 12 min 56 s. Positive and negative controls were twice-daily 0.12% chlorhexidine (CHX) and 0.89% NaCl, respectively.
26230333	7	12	used	used	1156:1159	arg2	microscopy					1101:1110	Variable pressure scanning electron microscopy	1065:1110	Variable pressure scanning electron microscopy	1065:1110	Variable pressure scanning electron microscopy and confocal scanning laser microscopy were used to check biofilm morphology and bacterial viability, respectively.
26230333	7	12	used	used	1156:1159	arg2	microscopy					1140:1149	confocal scanning laser microscopy	1116:1149	confocal scanning laser microscopy	1116:1149	Variable pressure scanning electron microscopy and confocal scanning laser microscopy were used to check biofilm morphology and bacterial viability, respectively.
26230333	3	13	theme	saliva-coated	543:555	arg1	discs					572:576	saliva-coated hydroxyapatite discs	543:576	saliva-coated hydroxyapatite discs for 5 days	543:587	METHODOLOGY/PRINCIPAL FINDINGS Biofilms of Streptococcus mutans UA159 were formed on saliva-coated hydroxyapatite discs for 5 days.
26230333	5	14	dep	Positive	822:829	arg1	s.					819:820	s.	819:820	s.	819:820	The distance between the light and the sample was 1.0 cm; energy density of 72 J cm-2; and exposure time of 12 min 56 s. Positive and negative controls were twice-daily 0.12% chlorhexidine (CHX) and 0.89% NaCl, respectively.
26230333	2	15	with	treatment	366:374	arg1	light					386:390	blue light	381:390	blue light	381:390	The aim of this study was to determine how the twice-daily treatment with blue light affects the development and composition of a matrix-rich biofilm.
26230333	8	16	theme	blue	1270:1273	arg1	light					1275:1279	twice-daily blue light	1258:1279	twice-daily blue light	1258:1279	When biofilms were exposed to twice-daily blue light, EPS-insoluble was reduced significantly more than in either control group (CHX and 0.89% NaCl).
26230333	11	17	dep	photosensitizer	1683:1697	arg1	mechanism					1714:1722	a promising mechanism	1702:1722	a promising mechanism for the inhibition of matrix-rich biofilm development	1702:1776	CONCLUSIONS Twice-daily treatment with blue light without a photosensitizer is a promising mechanism for the inhibition of matrix-rich biofilm development.
26230333	3	18	theme	FINDINGS	480:487	arg1	Biofilms					489:496	METHODOLOGY/PRINCIPAL FINDINGS Biofilms	458:496	METHODOLOGY/PRINCIPAL FINDINGS Biofilms of Streptococcus mutans UA159	458:526	METHODOLOGY/PRINCIPAL FINDINGS Biofilms of Streptococcus mutans UA159 were formed on saliva-coated hydroxyapatite discs for 5 days.
26230333	7	19	theme	bacterial	1193:1201	arg1	viability					1203:1211	bacterial viability	1193:1211	bacterial viability	1193:1211	Variable pressure scanning electron microscopy and confocal scanning laser microscopy were used to check biofilm morphology and bacterial viability, respectively.
26230333	5	20	theme	negative	835:842	arg1	controls					844:851	56 s. Positive and negative controls	816:851	56 s. Positive and negative controls	816:851	The distance between the light and the sample was 1.0 cm; energy density of 72 J cm-2; and exposure time of 12 min 56 s. Positive and negative controls were twice-daily 0.12% chlorhexidine (CHX) and 0.89% NaCl, respectively.
26230333	7	21	theme	pressure	1074:1081	arg1	microscopy					1101:1110	Variable pressure scanning electron microscopy	1065:1110	Variable pressure scanning electron microscopy	1065:1110	Variable pressure scanning electron microscopy and confocal scanning laser microscopy were used to check biofilm morphology and bacterial viability, respectively.
26230333	6	22	theme	extra	990:994	arg1	polysaccharides					1048:1062	extra (EPS-insoluble and soluble) and intracellular (IPS) polysaccharides	990:1062	extra (EPS-insoluble and soluble) and intracellular (IPS) polysaccharides	990:1062	Biofilms were analyzed for bacterial viability, dry-weight, and extra (EPS-insoluble and soluble) and intracellular (IPS) polysaccharides.
26230333	3	23	theme	mutans	515:520	arg1	Biofilms					489:496	METHODOLOGY/PRINCIPAL FINDINGS Biofilms	458:496	METHODOLOGY/PRINCIPAL FINDINGS Biofilms of Streptococcus mutans UA159	458:526	METHODOLOGY/PRINCIPAL FINDINGS Biofilms of Streptococcus mutans UA159 were formed on saliva-coated hydroxyapatite discs for 5 days.
26230333	10	24	theme	Different	1537:1545	arg1	morphology					1547:1556	Different morphology	1537:1556	Different morphology	1537:1556	Different morphology was also visible when the biofilms were treated with blue light.
26230333	6	25	theme	intracellular	1028:1040	arg1	polysaccharides					1048:1062	extra (EPS-insoluble and soluble) and intracellular (IPS) polysaccharides	990:1062	extra (EPS-insoluble and soluble) and intracellular (IPS) polysaccharides	990:1062	Biofilms were analyzed for bacterial viability, dry-weight, and extra (EPS-insoluble and soluble) and intracellular (IPS) polysaccharides.
26230333	5	26	theme	0.89	900:903	arg1	%					904:904	%	904:904	%	904:904	The distance between the light and the sample was 1.0 cm; energy density of 72 J cm-2; and exposure time of 12 min 56 s. Positive and negative controls were twice-daily 0.12% chlorhexidine (CHX) and 0.89% NaCl, respectively.
26230333	7	27	theme	scanning	1083:1090	arg1	microscopy					1101:1110	Variable pressure scanning electron microscopy	1065:1110	Variable pressure scanning electron microscopy	1065:1110	Variable pressure scanning electron microscopy and confocal scanning laser microscopy were used to check biofilm morphology and bacterial viability, respectively.
26230333	11	28	theme	biofilm	1758:1764	arg1	development					1766:1776	matrix-rich biofilm development	1746:1776	matrix-rich biofilm development	1746:1776	CONCLUSIONS Twice-daily treatment with blue light without a photosensitizer is a promising mechanism for the inhibition of matrix-rich biofilm development.
26230333	2	29	dep	development	404:414	arg1	the					400:402	the	400:402	the	400:402	The aim of this study was to determine how the twice-daily treatment with blue light affects the development and composition of a matrix-rich biofilm.
26230333	11	30	theme	promising	1704:1712	arg1	mechanism					1714:1722	a promising mechanism	1702:1722	a promising mechanism for the inhibition of matrix-rich biofilm development	1702:1776	CONCLUSIONS Twice-daily treatment with blue light without a photosensitizer is a promising mechanism for the inhibition of matrix-rich biofilm development.
26230333	3	31	theme	hydroxyapatite	557:570	arg1	discs					572:576	saliva-coated hydroxyapatite discs	543:576	saliva-coated hydroxyapatite discs for 5 days	543:587	METHODOLOGY/PRINCIPAL FINDINGS Biofilms of Streptococcus mutans UA159 were formed on saliva-coated hydroxyapatite discs for 5 days.
26230333	1	32	theme	local	161:165	arg1	infections					177:186	local bacterial infections	161:186	local bacterial infections	161:186	BACKGROUND The use of blue light has been proposed as a direct means of affecting local bacterial infections, however the use of blue light without a photosensitizer to prevent the biofilm development has not yet been explored.
26230333	7	33	theme	electron	1092:1099	arg1	microscopy					1101:1110	Variable pressure scanning electron microscopy	1065:1110	Variable pressure scanning electron microscopy	1065:1110	Variable pressure scanning electron microscopy and confocal scanning laser microscopy were used to check biofilm morphology and bacterial viability, respectively.
26230333	9	34	theme	%	1469:1469	arg1	NaCl					1471:1474	0.89% NaCl	1465:1474	0.89% NaCl	1465:1474	Bacterial viability and dry weight were also reduced relative to the negative control (0.89% NaCl) when the biofilms were treated with twice-daily blue light.
26230333	9	34	theme	%	1469:1469	arg1	control					1456:1462	the negative control	1443:1462	the negative control (0.89% NaCl)	1443:1475	Bacterial viability and dry weight were also reduced relative to the negative control (0.89% NaCl) when the biofilms were treated with twice-daily blue light.
26230333	9	35	theme	twice-daily	1513:1523	arg1	light					1530:1534	twice-daily blue light	1513:1534	twice-daily blue light	1513:1534	Bacterial viability and dry weight were also reduced relative to the negative control (0.89% NaCl) when the biofilms were treated with twice-daily blue light.
26230333	1	36	theme	bacterial	167:175	arg1	infections					177:186	local bacterial infections	161:186	local bacterial infections	161:186	BACKGROUND The use of blue light has been proposed as a direct means of affecting local bacterial infections, however the use of blue light without a photosensitizer to prevent the biofilm development has not yet been explored.
26230333	0	37	theme	Blue	22:25	arg1	Treatment					33:41	Twice-Daily Blue Light Treatment	10:41	Twice-Daily Blue Light Treatment	10:41	Effect of Twice-Daily Blue Light Treatment on Matrix-Rich Biofilm Development.
26230333	9	38	theme	blue	1525:1528	arg1	light					1530:1534	twice-daily blue light	1513:1534	twice-daily blue light	1513:1534	Bacterial viability and dry weight were also reduced relative to the negative control (0.89% NaCl) when the biofilms were treated with twice-daily blue light.
26230333	6	39	dep	extra	990:994	arg1	IPS					1043:1045	IPS	1043:1045	IPS	1043:1045	Biofilms were analyzed for bacterial viability, dry-weight, and extra (EPS-insoluble and soluble) and intracellular (IPS) polysaccharides.
26230333	6	39	dep	extra	990:994	arg1	soluble					1015:1021	soluble	1015:1021	soluble	1015:1021	Biofilms were analyzed for bacterial viability, dry-weight, and extra (EPS-insoluble and soluble) and intracellular (IPS) polysaccharides.
26230333	6	39	dep	extra	990:994	arg1	EPS-insoluble					997:1009	EPS-insoluble	997:1009	EPS-insoluble	997:1009	Biofilms were analyzed for bacterial viability, dry-weight, and extra (EPS-insoluble and soluble) and intracellular (IPS) polysaccharides.
26230333	2	40	theme	blue	381:384	arg1	light					386:390	blue light	381:390	blue light	381:390	The aim of this study was to determine how the twice-daily treatment with blue light affects the development and composition of a matrix-rich biofilm.
26230333	0	41	theme	Twice-Daily	10:20	arg1	Treatment					33:41	Twice-Daily Blue Light Treatment	10:41	Twice-Daily Blue Light Treatment	10:41	Effect of Twice-Daily Blue Light Treatment on Matrix-Rich Biofilm Development.
26230333	1	42	theme	biofilm	260:266	arg1	development					268:278	the biofilm development	256:278	the biofilm development	256:278	BACKGROUND The use of blue light has been proposed as a direct means of affecting local bacterial infections, however the use of blue light without a photosensitizer to prevent the biofilm development has not yet been explored.
26230333	8	43	theme	%	1369:1369	arg1	NaCl					1371:1374	0.89% NaCl	1365:1374	0.89% NaCl	1365:1374	When biofilms were exposed to twice-daily blue light, EPS-insoluble was reduced significantly more than in either control group (CHX and 0.89% NaCl).
26230333	1	44	theme	blue	101:104	arg1	light					106:110	blue light	101:110	blue light	101:110	BACKGROUND The use of blue light has been proposed as a direct means of affecting local bacterial infections, however the use of blue light without a photosensitizer to prevent the biofilm development has not yet been explored.
26230333	7	45	theme	biofilm	1170:1176	arg1	morphology					1178:1187	biofilm morphology	1170:1187	biofilm morphology	1170:1187	Variable pressure scanning electron microscopy and confocal scanning laser microscopy were used to check biofilm morphology and bacterial viability, respectively.
26230333	0	46	theme	Treatment	33:41	arg1	Effect					0:5	Effect	0:5	Effect of Twice-Daily Blue Light Treatment on Matrix-Rich Biofilm Development.	0:77	Effect of Twice-Daily Blue Light Treatment on Matrix-Rich Biofilm Development.
26230333	3	47	theme	METHODOLOGY/PRINCIPAL	458:478	arg1	Biofilms					489:496	METHODOLOGY/PRINCIPAL FINDINGS Biofilms	458:496	METHODOLOGY/PRINCIPAL FINDINGS Biofilms of Streptococcus mutans UA159	458:526	METHODOLOGY/PRINCIPAL FINDINGS Biofilms of Streptococcus mutans UA159 were formed on saliva-coated hydroxyapatite discs for 5 days.
26230333	11	48	theme	development	1766:1776	arg1	inhibition					1732:1741	the inhibition	1728:1741	the inhibition of matrix-rich biofilm development	1728:1776	CONCLUSIONS Twice-daily treatment with blue light without a photosensitizer is a promising mechanism for the inhibition of matrix-rich biofilm development.
26230333	6	49	theme	bacterial	953:961	arg1	viability					963:971	bacterial viability	953:971	bacterial viability	953:971	Biofilms were analyzed for bacterial viability, dry-weight, and extra (EPS-insoluble and soluble) and intracellular (IPS) polysaccharides.
26230333	4	50	theme	LumaCare	656:663	arg1	nm					670:671	LumaCare; 420 nm	656:671	LumaCare; 420 nm	656:671	The biofilms were exposed twice-daily to non-coherent blue light (LumaCare; 420 nm) without a photosensitizer.
26230333	4	50	theme	LumaCare	656:663	arg1	light					649:653	non-coherent blue light	631:653	non-coherent blue light (LumaCare; 420 nm)	631:672	The biofilms were exposed twice-daily to non-coherent blue light (LumaCare; 420 nm) without a photosensitizer.
26230333	4	51	theme	blue	644:647	arg1	nm					670:671	LumaCare; 420 nm	656:671	LumaCare; 420 nm	656:671	The biofilms were exposed twice-daily to non-coherent blue light (LumaCare; 420 nm) without a photosensitizer.
26230333	4	51	theme	blue	644:647	arg1	light					649:653	non-coherent blue light	631:653	non-coherent blue light (LumaCare; 420 nm)	631:672	The biofilms were exposed twice-daily to non-coherent blue light (LumaCare; 420 nm) without a photosensitizer.
26230333	0	52	theme	Light	27:31	arg1	Treatment					33:41	Twice-Daily Blue Light Treatment	10:41	Twice-Daily Blue Light Treatment	10:41	Effect of Twice-Daily Blue Light Treatment on Matrix-Rich Biofilm Development.
26230333	9	53	theme	Bacterial	1378:1386	arg1	viability					1388:1396	Bacterial viability	1378:1396	Bacterial viability	1378:1396	Bacterial viability and dry weight were also reduced relative to the negative control (0.89% NaCl) when the biofilms were treated with twice-daily blue light.
26230333	7	54	theme	Variable	1065:1072	arg1	microscopy					1101:1110	Variable pressure scanning electron microscopy	1065:1110	Variable pressure scanning electron microscopy	1065:1110	Variable pressure scanning electron microscopy and confocal scanning laser microscopy were used to check biofilm morphology and bacterial viability, respectively.
26230333	5	55	theme	exposure	792:799	arg1	time					801:804	exposure time	792:804	exposure time of 12 min	792:814	The distance between the light and the sample was 1.0 cm; energy density of 72 J cm-2; and exposure time of 12 min 56 s. Positive and negative controls were twice-daily 0.12% chlorhexidine (CHX) and 0.89% NaCl, respectively.
26230333	2	56	theme	twice-daily	354:364	arg1	treatment					366:374	the twice-daily treatment	350:374	the twice-daily treatment with blue light	350:390	The aim of this study was to determine how the twice-daily treatment with blue light affects the development and composition of a matrix-rich biofilm.
26230333	0	57	theme	Matrix-Rich	46:56	arg1	Development					66:76	Matrix-Rich Biofilm Development	46:76	Matrix-Rich Biofilm Development	46:76	Effect of Twice-Daily Blue Light Treatment on Matrix-Rich Biofilm Development.
26230333	8	58	theme	control	1342:1348	arg1	group					1350:1354	either control group	1335:1354	either control group (CHX and 0.89% NaCl)	1335:1375	When biofilms were exposed to twice-daily blue light, EPS-insoluble was reduced significantly more than in either control group (CHX and 0.89% NaCl).
26230333	11	59	theme	Twice-daily	1635:1645	arg1	treatment					1647:1655	Twice-daily treatment	1635:1655	Twice-daily treatment with blue light	1635:1671	CONCLUSIONS Twice-daily treatment with blue light without a photosensitizer is a promising mechanism for the inhibition of matrix-rich biofilm development.
26230333	1	60	theme	light	106:110	arg1	use					94:96	The use	90:96	The use of blue light	90:110	BACKGROUND The use of blue light has been proposed as a direct means of affecting local bacterial infections, however the use of blue light without a photosensitizer to prevent the biofilm development has not yet been explored.
26230333	1	60	theme	light	106:110	arg1	means					142:146	a direct means	133:146	a direct means of affecting local bacterial infections, however the use of blue light without a photosensitizer to prevent the biofilm development has not yet been explored	133:304	BACKGROUND The use of blue light has been proposed as a direct means of affecting local bacterial infections, however the use of blue light without a photosensitizer to prevent the biofilm development has not yet been explored.
26230333	5	61	theme	energy	759:764	arg1	density					766:772	energy density	759:772	energy density of 72 J cm-2	759:785	The distance between the light and the sample was 1.0 cm; energy density of 72 J cm-2; and exposure time of 12 min 56 s. Positive and negative controls were twice-daily 0.12% chlorhexidine (CHX) and 0.89% NaCl, respectively.
26230333	11	62	with	treatment	1647:1655	arg1	light					1667:1671	blue light	1662:1671	blue light	1662:1671	CONCLUSIONS Twice-daily treatment with blue light without a photosensitizer is a promising mechanism for the inhibition of matrix-rich biofilm development.
26230333	7	63	theme	scanning	1125:1132	arg1	microscopy					1140:1149	confocal scanning laser microscopy	1116:1149	confocal scanning laser microscopy	1116:1149	Variable pressure scanning electron microscopy and confocal scanning laser microscopy were used to check biofilm morphology and bacterial viability, respectively.
26230333	1	64	dep	BACKGROUND	79:88	arg1	proposed					121:128	proposed	121:128	has been proposed as a direct means of affecting local bacterial infections, however the use of blue light without a photosensitizer to prevent the biofilm development has not yet been explored	112:304	BACKGROUND The use of blue light has been proposed as a direct means of affecting local bacterial infections, however the use of blue light without a photosensitizer to prevent the biofilm development has not yet been explored.
26230333	5	65	theme	%	904:904	arg1	NaCl					906:909	0.89% NaCl	900:909	0.89% NaCl	900:909	The distance between the light and the sample was 1.0 cm; energy density of 72 J cm-2; and exposure time of 12 min 56 s. Positive and negative controls were twice-daily 0.12% chlorhexidine (CHX) and 0.89% NaCl, respectively.
26230333	11	66	theme	matrix-rich	1746:1756	arg1	development					1766:1776	matrix-rich biofilm development	1746:1776	matrix-rich biofilm development	1746:1776	CONCLUSIONS Twice-daily treatment with blue light without a photosensitizer is a promising mechanism for the inhibition of matrix-rich biofilm development.
26230333	5	67	theme	min	812:814	arg1	time					801:804	exposure time	792:804	exposure time of 12 min	792:814	The distance between the light and the sample was 1.0 cm; energy density of 72 J cm-2; and exposure time of 12 min 56 s. Positive and negative controls were twice-daily 0.12% chlorhexidine (CHX) and 0.89% NaCl, respectively.
26230333	5	67	theme	min	812:814	arg1	density					766:772	energy density	759:772	energy density of 72 J cm-2	759:785	The distance between the light and the sample was 1.0 cm; energy density of 72 J cm-2; and exposure time of 12 min 56 s. Positive and negative controls were twice-daily 0.12% chlorhexidine (CHX) and 0.89% NaCl, respectively.
26230333	8	68	dep	group	1350:1354	arg1	NaCl					1371:1374	0.89% NaCl	1365:1374	0.89% NaCl	1365:1374	When biofilms were exposed to twice-daily blue light, EPS-insoluble was reduced significantly more than in either control group (CHX and 0.89% NaCl).
26230333	8	68	dep	group	1350:1354	arg1	CHX					1357:1359	CHX	1357:1359	CHX	1357:1359	When biofilms were exposed to twice-daily blue light, EPS-insoluble was reduced significantly more than in either control group (CHX and 0.89% NaCl).
26230333	9	69	theme	negative	1447:1454	arg1	NaCl					1471:1474	0.89% NaCl	1465:1474	0.89% NaCl	1465:1474	Bacterial viability and dry weight were also reduced relative to the negative control (0.89% NaCl) when the biofilms were treated with twice-daily blue light.
26230333	9	69	theme	negative	1447:1454	arg1	control					1456:1462	the negative control	1443:1462	the negative control (0.89% NaCl)	1443:1475	Bacterial viability and dry weight were also reduced relative to the negative control (0.89% NaCl) when the biofilms were treated with twice-daily blue light.
26230333	3	70	theme	Streptococcus	501:513	arg1	mutans					515:520	Streptococcus mutans UA159	501:526	Streptococcus mutans UA159	501:526	METHODOLOGY/PRINCIPAL FINDINGS Biofilms of Streptococcus mutans UA159 were formed on saliva-coated hydroxyapatite discs for 5 days.
26230333	8	71	theme	twice-daily	1258:1268	arg1	light					1275:1279	twice-daily blue light	1258:1279	twice-daily blue light	1258:1279	When biofilms were exposed to twice-daily blue light, EPS-insoluble was reduced significantly more than in either control group (CHX and 0.89% NaCl).
26230333	11	72	theme	blue	1662:1665	arg1	light					1667:1671	blue light	1662:1671	blue light	1662:1671	CONCLUSIONS Twice-daily treatment with blue light without a photosensitizer is a promising mechanism for the inhibition of matrix-rich biofilm development.
26230333	4	73	theme	non-coherent	631:642	arg1	nm					670:671	LumaCare; 420 nm	656:671	LumaCare; 420 nm	656:671	The biofilms were exposed twice-daily to non-coherent blue light (LumaCare; 420 nm) without a photosensitizer.
26230333	4	73	theme	non-coherent	631:642	arg1	light					649:653	non-coherent blue light	631:653	non-coherent blue light (LumaCare; 420 nm)	631:672	The biofilms were exposed twice-daily to non-coherent blue light (LumaCare; 420 nm) without a photosensitizer.
26230333	1	74	dep	infections	177:186	arg1	explored					297:304	explored	297:304	has not yet been explored	280:304	BACKGROUND The use of blue light has been proposed as a direct means of affecting local bacterial infections, however the use of blue light without a photosensitizer to prevent the biofilm development has not yet been explored.
26230333	5	75	theme	cm-2	782:785	arg1	time					801:804	exposure time	792:804	exposure time of 12 min	792:814	The distance between the light and the sample was 1.0 cm; energy density of 72 J cm-2; and exposure time of 12 min 56 s. Positive and negative controls were twice-daily 0.12% chlorhexidine (CHX) and 0.89% NaCl, respectively.
26230333	5	75	theme	cm-2	782:785	arg1	density					766:772	energy density	759:772	energy density of 72 J cm-2	759:785	The distance between the light and the sample was 1.0 cm; energy density of 72 J cm-2; and exposure time of 12 min 56 s. Positive and negative controls were twice-daily 0.12% chlorhexidine (CHX) and 0.89% NaCl, respectively.
26230333	5	76	dep	chlorhexidine	876:888	arg1	%					874:874	%	874:874	%	874:874	The distance between the light and the sample was 1.0 cm; energy density of 72 J cm-2; and exposure time of 12 min 56 s. Positive and negative controls were twice-daily 0.12% chlorhexidine (CHX) and 0.89% NaCl, respectively.
26230333	1	77	theme	blue	208:211	arg1	light					213:217	blue light	208:217	blue light	208:217	BACKGROUND The use of blue light has been proposed as a direct means of affecting local bacterial infections, however the use of blue light without a photosensitizer to prevent the biofilm development has not yet been explored.
26230333	0	78	theme	Biofilm	58:64	arg1	Development					66:76	Matrix-Rich Biofilm Development	46:76	Matrix-Rich Biofilm Development	46:76	Effect of Twice-Daily Blue Light Treatment on Matrix-Rich Biofilm Development.
26230333	5	79	theme	J	780:780	arg1	cm-2					782:785	72 J cm-2	777:785	72 J cm-2	777:785	The distance between the light and the sample was 1.0 cm; energy density of 72 J cm-2; and exposure time of 12 min 56 s. Positive and negative controls were twice-daily 0.12% chlorhexidine (CHX) and 0.89% NaCl, respectively.
26230333	2	80	theme	study	323:327	arg1	aim					311:313	The aim	307:313	The aim of this study	307:327	The aim of this study was to determine how the twice-daily treatment with blue light affects the development and composition of a matrix-rich biofilm.
26230333	1	81	theme	light	213:217	arg1	use					201:203	the use	197:203	the use of blue light without a photosensitizer to prevent the biofilm development	197:278	BACKGROUND The use of blue light has been proposed as a direct means of affecting local bacterial infections, however the use of blue light without a photosensitizer to prevent the biofilm development has not yet been explored.
27516292	6	0	theme	acid	832:835	arg1	permeability					810:821	The permeability	806:821	The permeability of oleic acid	806:835	The permeability of oleic acid was however unchanged, which is most probable a result of high solubility in the cellulose ester rich domains of the composites.
27516292	6	0	theme	acid	832:835	arg1	unchanged					849:857	unchanged	849:857	unchanged	849:857	The permeability of oleic acid was however unchanged, which is most probable a result of high solubility in the cellulose ester rich domains of the composites.
27516292	1	1	theme	carbon	147:152	arg1	chains					159:164	long carbon side chains	142:164	long carbon side chains (e.g. stearate)	142:180	Cellulose esters with long carbon side chains (e.g. stearate) were produced via a homogenous reaction in ionic liquids.
27516292	1	2	theme	ionic	225:229	arg1	liquids					231:237	ionic liquids	225:237	ionic liquids	225:237	Cellulose esters with long carbon side chains (e.g. stearate) were produced via a homogenous reaction in ionic liquids.
27516292	0	3	theme	phase	59:63	arg1	films					50:54	composite films	40:54	composite films of phase	40:63	Permeability of water and oleic acid in composite films of phase separated polypropylene and cellulose stearate blends.
27516292	7	4	theme	crystallization	1055:1069	arg1	process					1071:1077	the polypropylene crystallization process	1037:1077	the polypropylene crystallization process	1037:1077	Also, the following hypothesis is stated: cellulose stearate influence the polypropylene crystallization process by decreasing the size of spherulites.
27516292	7	5	theme	cellulose	1008:1016	arg1	stearate					1018:1025	cellulose stearate	1008:1025	cellulose stearate	1008:1025	Also, the following hypothesis is stated: cellulose stearate influence the polypropylene crystallization process by decreasing the size of spherulites.
27516292	1	6	theme	side	154:157	arg1	chains					159:164	long carbon side chains	142:164	long carbon side chains (e.g. stearate)	142:180	Cellulose esters with long carbon side chains (e.g. stearate) were produced via a homogenous reaction in ionic liquids.
27516292	6	7	theme	rich	934:937	arg1	domains					939:945	the cellulose ester rich domains	914:945	the cellulose ester rich domains of the composites	914:963	The permeability of oleic acid was however unchanged, which is most probable a result of high solubility in the cellulose ester rich domains of the composites.
27516292	6	7	theme	rich	934:937	arg1	composites					954:963	the composites	950:963	the composites	950:963	The permeability of oleic acid was however unchanged, which is most probable a result of high solubility in the cellulose ester rich domains of the composites.
27516292	7	8	theme	spherulites	1105:1115	arg1	size					1097:1100	the size	1093:1100	the size of spherulites	1093:1115	Also, the following hypothesis is stated: cellulose stearate influence the polypropylene crystallization process by decreasing the size of spherulites.
27516292	0	9	from	Permeability	0:11	arg1	films					50:54	composite films	40:54	composite films of phase	40:63	Permeability of water and oleic acid in composite films of phase separated polypropylene and cellulose stearate blends.
27516292	7	10	theme	polypropylene	1041:1053	arg1	process					1071:1077	the polypropylene crystallization process	1037:1077	the polypropylene crystallization process	1037:1077	Also, the following hypothesis is stated: cellulose stearate influence the polypropylene crystallization process by decreasing the size of spherulites.
27516292	7	11	theme	following	976:984	arg1	hypothesis					986:995	the following hypothesis	972:995	the following hypothesis	972:995	Also, the following hypothesis is stated: cellulose stearate influence the polypropylene crystallization process by decreasing the size of spherulites.
27516292	6	12	theme	ester	928:932	arg1	domains					939:945	the cellulose ester rich domains	914:945	the cellulose ester rich domains of the composites	914:963	The permeability of oleic acid was however unchanged, which is most probable a result of high solubility in the cellulose ester rich domains of the composites.
27516292	6	12	theme	ester	928:932	arg1	composites					954:963	the composites	950:963	the composites	950:963	The permeability of oleic acid was however unchanged, which is most probable a result of high solubility in the cellulose ester rich domains of the composites.
27516292	0	13	theme	polypropylene	75:87	arg1	blends					112:117	polypropylene and cellulose stearate blends	75:117	blends	112:117	Permeability of water and oleic acid in composite films of phase separated polypropylene and cellulose stearate blends.
27516292	5	14	theme	water	641:645	arg1	permeability					625:636	permeability	625:636	permeability	625:636	The morphology and permeability of water and oleic acid of the composite films were studied and it was shown that the water permeability decreased upon addition of the cellulose ester to the polymer.
27516292	5	14	theme	water	641:645	arg1	morphology					610:619	morphology	610:619	morphology	610:619	The morphology and permeability of water and oleic acid of the composite films were studied and it was shown that the water permeability decreased upon addition of the cellulose ester to the polymer.
27516292	2	15	theme	substitution	254:265	arg1	degree					244:249	The degree	240:249	The degree of substitution	240:265	The degree of substitution was calculated to approximately 2.
27516292	6	16	theme	cellulose	918:926	arg1	domains					939:945	the cellulose ester rich domains	914:945	the cellulose ester rich domains of the composites	914:963	The permeability of oleic acid was however unchanged, which is most probable a result of high solubility in the cellulose ester rich domains of the composites.
27516292	6	16	theme	cellulose	918:926	arg1	composites					954:963	the composites	950:963	the composites	950:963	The permeability of oleic acid was however unchanged, which is most probable a result of high solubility in the cellulose ester rich domains of the composites.
27516292	0	17	theme	stearate	103:110	arg1	blends					112:117	polypropylene and cellulose stearate blends	75:117	blends	112:117	Permeability of water and oleic acid in composite films of phase separated polypropylene and cellulose stearate blends.
27516292	3	18	theme	polypropylene	402:414	arg1	combinations					371:382	combinations	371:382	combinations of the esters and polypropylene	371:414	The melt rheology was studied for the pure cellulose esters but also combinations of the esters and polypropylene to study the processability of a blended composite material.
27516292	0	19	theme	cellulose	93:101	arg1	blends					112:117	polypropylene and cellulose stearate blends	75:117	blends	112:117	Permeability of water and oleic acid in composite films of phase separated polypropylene and cellulose stearate blends.
27516292	1	20	with	esters	130:135	arg1	chains					159:164	long carbon side chains	142:164	long carbon side chains (e.g. stearate)	142:180	Cellulose esters with long carbon side chains (e.g. stearate) were produced via a homogenous reaction in ionic liquids.
27516292	6	21	theme	composites	954:963	arg1	domains					939:945	the cellulose ester rich domains	914:945	the cellulose ester rich domains of the composites	914:963	The permeability of oleic acid was however unchanged, which is most probable a result of high solubility in the cellulose ester rich domains of the composites.
27516292	6	21	theme	composites	954:963	arg1	composites					954:963	the composites	950:963	the composites	950:963	The permeability of oleic acid was however unchanged, which is most probable a result of high solubility in the cellulose ester rich domains of the composites.
27516292	5	22	theme	water	724:728	arg1	permeability					730:741	the water permeability	720:741	the water permeability	720:741	The morphology and permeability of water and oleic acid of the composite films were studied and it was shown that the water permeability decreased upon addition of the cellulose ester to the polymer.
27516292	6	23	from	domains	939:945	arg1	result					885:890	most probable a result	869:890	most probable a result of high solubility in the cellulose ester rich domains of the composites	869:963	The permeability of oleic acid was however unchanged, which is most probable a result of high solubility in the cellulose ester rich domains of the composites.
27516292	6	23	from	domains	939:945	arg1	which					860:864	which	860:864	which	860:864	The permeability of oleic acid was however unchanged, which is most probable a result of high solubility in the cellulose ester rich domains of the composites.
27516292	5	24	theme	oleic	651:655	arg1	acid					657:660	oleic acid	651:660	oleic acid	651:660	The morphology and permeability of water and oleic acid of the composite films were studied and it was shown that the water permeability decreased upon addition of the cellulose ester to the polymer.
27516292	3	25	theme	esters	391:396	arg1	combinations					371:382	combinations	371:382	combinations of the esters and polypropylene	371:414	The melt rheology was studied for the pure cellulose esters but also combinations of the esters and polypropylene to study the processability of a blended composite material.
27516292	3	26	theme	pure	340:343	arg1	esters					355:360	the pure cellulose esters	336:360	the pure cellulose esters	336:360	The melt rheology was studied for the pure cellulose esters but also combinations of the esters and polypropylene to study the processability of a blended composite material.
27516292	6	27	from	solubility	900:909	arg1	domains					939:945	the cellulose ester rich domains	914:945	the cellulose ester rich domains of the composites	914:963	The permeability of oleic acid was however unchanged, which is most probable a result of high solubility in the cellulose ester rich domains of the composites.
27516292	6	27	from	solubility	900:909	arg1	composites					954:963	the composites	950:963	the composites	950:963	The permeability of oleic acid was however unchanged, which is most probable a result of high solubility in the cellulose ester rich domains of the composites.
27516292	3	28	theme	cellulose	345:353	arg1	esters					355:360	the pure cellulose esters	336:360	the pure cellulose esters	336:360	The melt rheology was studied for the pure cellulose esters but also combinations of the esters and polypropylene to study the processability of a blended composite material.
27516292	6	29	theme	solubility	900:909	arg1	result					885:890	most probable a result	869:890	most probable a result of high solubility in the cellulose ester rich domains of the composites	869:963	The permeability of oleic acid was however unchanged, which is most probable a result of high solubility in the cellulose ester rich domains of the composites.
27516292	6	29	theme	solubility	900:909	arg1	which					860:864	which	860:864	which	860:864	The permeability of oleic acid was however unchanged, which is most probable a result of high solubility in the cellulose ester rich domains of the composites.
27516292	5	30	theme	composite	669:677	arg1	films					679:683	the composite films	665:683	the composite films	665:683	The morphology and permeability of water and oleic acid of the composite films were studied and it was shown that the water permeability decreased upon addition of the cellulose ester to the polymer.
27516292	6	31	theme	oleic	826:830	arg1	acid					832:835	oleic acid	826:835	oleic acid	826:835	The permeability of oleic acid was however unchanged, which is most probable a result of high solubility in the cellulose ester rich domains of the composites.
27516292	0	32	theme	acid	32:35	arg1	Permeability					0:11	Permeability	0:11	Permeability of water and oleic acid in composite films of phase	0:63	Permeability of water and oleic acid in composite films of phase separated polypropylene and cellulose stearate blends.
27516292	6	33	theme	high	895:898	arg1	solubility					900:909	high solubility	895:909	high solubility in the cellulose ester rich domains of the composites	895:963	The permeability of oleic acid was however unchanged, which is most probable a result of high solubility in the cellulose ester rich domains of the composites.
27516292	5	34	theme	films	679:683	arg1	acid					657:660	oleic acid	651:660	oleic acid	651:660	The morphology and permeability of water and oleic acid of the composite films were studied and it was shown that the water permeability decreased upon addition of the cellulose ester to the polymer.
27516292	5	34	theme	films	679:683	arg1	water					641:645	water	641:645	water	641:645	The morphology and permeability of water and oleic acid of the composite films were studied and it was shown that the water permeability decreased upon addition of the cellulose ester to the polymer.
27516292	0	35	theme	oleic	26:30	arg1	acid					32:35	oleic acid	26:35	oleic acid	26:35	Permeability of water and oleic acid in composite films of phase separated polypropylene and cellulose stearate blends.
27516292	5	36	dep	morphology	610:619	arg1	The					606:608	The	606:608	The	606:608	The morphology and permeability of water and oleic acid of the composite films were studied and it was shown that the water permeability decreased upon addition of the cellulose ester to the polymer.
27516292	1	37	dep	stearate	172:179	arg1	e.g.					167:170	e.g.	167:170	e.g.	167:170	Cellulose esters with long carbon side chains (e.g. stearate) were produced via a homogenous reaction in ionic liquids.
27516292	5	38	theme	cellulose	774:782	arg1	ester					784:788	the cellulose ester	770:788	the cellulose ester	770:788	The morphology and permeability of water and oleic acid of the composite films were studied and it was shown that the water permeability decreased upon addition of the cellulose ester to the polymer.
27516292	0	39	theme	composite	40:48	arg1	films					50:54	composite films	40:54	composite films of phase	40:63	Permeability of water and oleic acid in composite films of phase separated polypropylene and cellulose stearate blends.
27516292	1	40	from	reaction	213:220	arg1	liquids					231:237	ionic liquids	225:237	ionic liquids	225:237	Cellulose esters with long carbon side chains (e.g. stearate) were produced via a homogenous reaction in ionic liquids.
27516292	3	41	theme	composite	457:465	arg1	material					467:474	a blended composite material	447:474	a blended composite material	447:474	The melt rheology was studied for the pure cellulose esters but also combinations of the esters and polypropylene to study the processability of a blended composite material.
27516292	3	42	dep	studied	324:330	arg1	esters					355:360	the pure cellulose esters	336:360	the pure cellulose esters	336:360	The melt rheology was studied for the pure cellulose esters but also combinations of the esters and polypropylene to study the processability of a blended composite material.
27516292	3	42	dep	studied	324:330	arg1	study					419:423	study	419:423	to study the processability of a blended composite material	416:474	The melt rheology was studied for the pure cellulose esters but also combinations of the esters and polypropylene to study the processability of a blended composite material.
27516292	3	43	theme	material	467:474	arg1	processability					429:442	the processability	425:442	the processability of a blended composite material	425:474	The melt rheology was studied for the pure cellulose esters but also combinations of the esters and polypropylene to study the processability of a blended composite material.
27516292	6	44	theme	probable	874:881	arg1	result					885:890	most probable a result	869:890	most probable a result of high solubility in the cellulose ester rich domains of the composites	869:963	The permeability of oleic acid was however unchanged, which is most probable a result of high solubility in the cellulose ester rich domains of the composites.
27516292	6	44	theme	probable	874:881	arg1	which					860:864	which	860:864	which	860:864	The permeability of oleic acid was however unchanged, which is most probable a result of high solubility in the cellulose ester rich domains of the composites.
27516292	5	45	theme	acid	657:660	arg1	permeability					625:636	permeability	625:636	permeability	625:636	The morphology and permeability of water and oleic acid of the composite films were studied and it was shown that the water permeability decreased upon addition of the cellulose ester to the polymer.
27516292	5	45	theme	acid	657:660	arg1	morphology					610:619	morphology	610:619	morphology	610:619	The morphology and permeability of water and oleic acid of the composite films were studied and it was shown that the water permeability decreased upon addition of the cellulose ester to the polymer.
27516292	1	46	theme	Cellulose	120:128	arg1	esters					130:135	Cellulose esters	120:135	Cellulose esters with long carbon side chains (e.g. stearate)	120:180	Cellulose esters with long carbon side chains (e.g. stearate) were produced via a homogenous reaction in ionic liquids.
27516292	6	47	from	result	885:890	arg1	domains					939:945	the cellulose ester rich domains	914:945	the cellulose ester rich domains of the composites	914:963	The permeability of oleic acid was however unchanged, which is most probable a result of high solubility in the cellulose ester rich domains of the composites.
27516292	6	47	from	result	885:890	arg1	composites					954:963	the composites	950:963	the composites	950:963	The permeability of oleic acid was however unchanged, which is most probable a result of high solubility in the cellulose ester rich domains of the composites.
27516292	5	48	theme	ester	784:788	arg1	addition					758:765	addition	758:765	addition of the cellulose ester to the polymer	758:803	The morphology and permeability of water and oleic acid of the composite films were studied and it was shown that the water permeability decreased upon addition of the cellulose ester to the polymer.
27516292	3	49	theme	blended	449:455	arg1	material					467:474	a blended composite material	447:474	a blended composite material	447:474	The melt rheology was studied for the pure cellulose esters but also combinations of the esters and polypropylene to study the processability of a blended composite material.
27516292	1	50	theme	homogenous	202:211	arg1	reaction					213:220	a homogenous reaction	200:220	a homogenous reaction in ionic liquids	200:237	Cellulose esters with long carbon side chains (e.g. stearate) were produced via a homogenous reaction in ionic liquids.
27516292	3	51	theme	melt	306:309	arg1	rheology					311:318	The melt rheology	302:318	The melt rheology	302:318	The melt rheology was studied for the pure cellulose esters but also combinations of the esters and polypropylene to study the processability of a blended composite material.
27516292	4	52	theme	composite	586:594	arg1	material					596:603	a phase-separated composite material	568:603	a phase-separated composite material	568:603	It was shown that the compatibility between the two components was weak, which resulted in a phase-separated composite material.
27516292	0	53	theme	water	16:20	arg1	Permeability					0:11	Permeability	0:11	Permeability of water and oleic acid in composite films of phase	0:63	Permeability of water and oleic acid in composite films of phase separated polypropylene and cellulose stearate blends.
27516292	1	54	theme	long	142:145	arg1	chains					159:164	long carbon side chains	142:164	long carbon side chains (e.g. stearate)	142:180	Cellulose esters with long carbon side chains (e.g. stearate) were produced via a homogenous reaction in ionic liquids.
27516292	4	55	theme	phase-separated	570:584	arg1	material					596:603	a phase-separated composite material	568:603	a phase-separated composite material	568:603	It was shown that the compatibility between the two components was weak, which resulted in a phase-separated composite material.
28325341	8	0	theme	quinoa	1228:1233	arg1	starch					1235:1240	quinoa starch	1228:1240	quinoa starch	1228:1240	This work highlights some relationships among composition, amylopectin structure and physicochemical properties of quinoa starch.
28325341	1	1	theme	interest	175:182	arg1	subject					155:161	a subject	153:161	a subject of research interest	153:182	Structure-function relationships of starch components remain a subject of research interest.
28325341	0	2	theme	starch	84:89	arg1	properties					63:72	physicochemical properties	47:72	physicochemical properties of quinoa starch	47:89	Amylopectin molecular structure in relation to physicochemical properties of quinoa starch.
28325341	4	3	theme	solubility	545:554	arg1	index					556:560	water solubility index	539:560	water solubility index	539:560	Pearson correlation analysis revealed that the properties related to gelatinization such as swelling power, water solubility index, crystallinity, pasting, and thermal properties are much affected by the amylopectin chain profile and amylose content.
28325341	7	4	theme	chain	1042:1046	arg1	profile					1048:1054	individual unit chain profile	1026:1054	individual unit chain profile	1026:1054	Interesting findings indicate the importance of amylopectin internal structure and individual unit chain profile in determining the physicochemical properties of starch.
28325341	4	5	theme	related	489:495	arg1	pasting					578:584	pasting	578:584	pasting	578:584	Pearson correlation analysis revealed that the properties related to gelatinization such as swelling power, water solubility index, crystallinity, pasting, and thermal properties are much affected by the amylopectin chain profile and amylose content.
28325341	4	5	theme	related	489:495	arg1	index					556:560	water solubility index	539:560	water solubility index	539:560	Pearson correlation analysis revealed that the properties related to gelatinization such as swelling power, water solubility index, crystallinity, pasting, and thermal properties are much affected by the amylopectin chain profile and amylose content.
28325341	4	5	theme	related	489:495	arg1	crystallinity					563:575	crystallinity	563:575	crystallinity	563:575	Pearson correlation analysis revealed that the properties related to gelatinization such as swelling power, water solubility index, crystallinity, pasting, and thermal properties are much affected by the amylopectin chain profile and amylose content.
28325341	4	5	theme	related	489:495	arg1	properties					478:487	the properties	474:487	the properties related to gelatinization such as swelling power, water solubility index, crystallinity, pasting, and thermal properties	474:608	Pearson correlation analysis revealed that the properties related to gelatinization such as swelling power, water solubility index, crystallinity, pasting, and thermal properties are much affected by the amylopectin chain profile and amylose content.
28325341	4	5	theme	related	489:495	arg1	properties					599:608	thermal properties	591:608	thermal properties	591:608	Pearson correlation analysis revealed that the properties related to gelatinization such as swelling power, water solubility index, crystallinity, pasting, and thermal properties are much affected by the amylopectin chain profile and amylose content.
28325341	4	5	theme	related	489:495	arg1	power					532:536	swelling power	523:536	swelling power	523:536	Pearson correlation analysis revealed that the properties related to gelatinization such as swelling power, water solubility index, crystallinity, pasting, and thermal properties are much affected by the amylopectin chain profile and amylose content.
28325341	4	6	theme	water	539:543	arg1	index					556:560	water solubility index	539:560	water solubility index	539:560	Pearson correlation analysis revealed that the properties related to gelatinization such as swelling power, water solubility index, crystallinity, pasting, and thermal properties are much affected by the amylopectin chain profile and amylose content.
28325341	6	7	theme	size	845:848	arg1	distribution					850:861	particle size distribution	836:861	particle size distribution	836:861	Other properties such as enzyme susceptibility and particle size distribution are also strongly correlated with starch composition and amylopectin structure.
28325341	5	8	theme	amylopectin	753:763	arg1	structure					774:782	amylopectin internal structure	753:782	amylopectin internal structure	753:782	The parameters of gel texture and amylose leaching are much related to amylopectin internal structure.
28325341	4	9	theme	Pearson	431:437	arg1	correlation					439:449	Pearson correlation	431:449	Pearson correlation analysis	431:458	Pearson correlation analysis revealed that the properties related to gelatinization such as swelling power, water solubility index, crystallinity, pasting, and thermal properties are much affected by the amylopectin chain profile and amylose content.
28325341	3	10	theme	physicochemical	336:350	arg1	properties					352:361	physicochemical properties	336:361	physicochemical properties	336:361	In this study, nine quinoa starches varied greatly in composition, structure, and physicochemical properties were selected for the analysis of structure-function relationships.
28325341	6	11	theme	particle	836:843	arg1	distribution					850:861	particle size distribution	836:861	particle size distribution	836:861	Other properties such as enzyme susceptibility and particle size distribution are also strongly correlated with starch composition and amylopectin structure.
28325341	0	12	theme	physicochemical	47:61	arg1	properties					63:72	physicochemical properties	47:72	physicochemical properties of quinoa starch	47:89	Amylopectin molecular structure in relation to physicochemical properties of quinoa starch.
28325341	4	13	theme	swelling	523:530	arg1	power					532:536	swelling power	523:536	swelling power	523:536	Pearson correlation analysis revealed that the properties related to gelatinization such as swelling power, water solubility index, crystallinity, pasting, and thermal properties are much affected by the amylopectin chain profile and amylose content.
28325341	6	14	theme	starch	897:902	arg1	composition					904:914	starch composition	897:914	starch composition	897:914	Other properties such as enzyme susceptibility and particle size distribution are also strongly correlated with starch composition and amylopectin structure.
28325341	5	15	theme	internal	765:772	arg1	structure					774:782	amylopectin internal structure	753:782	amylopectin internal structure	753:782	The parameters of gel texture and amylose leaching are much related to amylopectin internal structure.
28325341	0	16	theme	molecular	12:20	arg1	structure					22:30	molecular structure	12:30	molecular structure	12:30	Amylopectin molecular structure in relation to physicochemical properties of quinoa starch.
28325341	1	17	theme	Structure-function	92:109	arg1	relationships					111:123	Structure-function relationships	92:123	Structure-function relationships of starch components	92:144	Structure-function relationships of starch components remain a subject of research interest.
28325341	2	18	contain	has	199:201	arg2	∼2μm					224:227	∼2μm	224:227	∼2μm	224:227	Quinoa starch has very small granules (∼2μm) with unique properties.
28325341	2	18	contain	has	199:201	arg2	granules					214:221	very small granules	203:221	very small granules (∼2μm)	203:228	Quinoa starch has very small granules (∼2μm) with unique properties.
28325341	2	18	contain	has	199:201	arg1	starch					192:197	Quinoa starch	185:197	Quinoa starch	185:197	Quinoa starch has very small granules (∼2μm) with unique properties.
28325341	6	19	theme	enzyme	810:815	arg1	susceptibility					817:830	enzyme susceptibility	810:830	enzyme susceptibility	810:830	Other properties such as enzyme susceptibility and particle size distribution are also strongly correlated with starch composition and amylopectin structure.
28325341	7	20	theme	individual	1026:1035	arg1	profile					1048:1054	individual unit chain profile	1026:1054	individual unit chain profile	1026:1054	Interesting findings indicate the importance of amylopectin internal structure and individual unit chain profile in determining the physicochemical properties of starch.
28325341	7	21	theme	amylopectin	991:1001	arg1	structure					1012:1020	amylopectin internal structure	991:1020	amylopectin internal structure	991:1020	Interesting findings indicate the importance of amylopectin internal structure and individual unit chain profile in determining the physicochemical properties of starch.
28325341	3	22	theme	quinoa	274:279	arg1	starches					281:288	nine quinoa starches	269:288	nine quinoa starches varied greatly in composition, structure, and physicochemical properties	269:361	In this study, nine quinoa starches varied greatly in composition, structure, and physicochemical properties were selected for the analysis of structure-function relationships.
28325341	7	23	theme	unit	1037:1040	arg1	profile					1048:1054	individual unit chain profile	1026:1054	individual unit chain profile	1026:1054	Interesting findings indicate the importance of amylopectin internal structure and individual unit chain profile in determining the physicochemical properties of starch.
28325341	4	24	theme	thermal	591:597	arg1	properties					599:608	thermal properties	591:608	thermal properties	591:608	Pearson correlation analysis revealed that the properties related to gelatinization such as swelling power, water solubility index, crystallinity, pasting, and thermal properties are much affected by the amylopectin chain profile and amylose content.
28325341	8	25	theme	amylopectin	1172:1182	arg1	structure					1184:1192	amylopectin structure	1172:1192	amylopectin structure	1172:1192	This work highlights some relationships among composition, amylopectin structure and physicochemical properties of quinoa starch.
28325341	6	26	theme	amylopectin	920:930	arg1	structure					932:940	amylopectin structure	920:940	amylopectin structure	920:940	Other properties such as enzyme susceptibility and particle size distribution are also strongly correlated with starch composition and amylopectin structure.
28325341	1	27	theme	starch	128:133	arg1	components					135:144	starch components	128:144	starch components	128:144	Structure-function relationships of starch components remain a subject of research interest.
28325341	8	28	theme	physicochemical	1198:1212	arg1	properties					1214:1223	physicochemical properties	1198:1223	physicochemical properties	1198:1223	This work highlights some relationships among composition, amylopectin structure and physicochemical properties of quinoa starch.
28325341	2	29	theme	unique	235:240	arg1	properties					242:251	unique properties	235:251	unique properties	235:251	Quinoa starch has very small granules (∼2μm) with unique properties.
28325341	7	30	theme	starch	1105:1110	arg1	properties					1091:1100	the physicochemical properties	1071:1100	the physicochemical properties of starch	1071:1110	Interesting findings indicate the importance of amylopectin internal structure and individual unit chain profile in determining the physicochemical properties of starch.
28325341	4	31	theme	amylose	665:671	arg1	content					673:679	amylose content	665:679	amylose content	665:679	Pearson correlation analysis revealed that the properties related to gelatinization such as swelling power, water solubility index, crystallinity, pasting, and thermal properties are much affected by the amylopectin chain profile and amylose content.
28325341	3	32	theme	structure-function	397:414	arg1	relationships					416:428	structure-function relationships	397:428	structure-function relationships	397:428	In this study, nine quinoa starches varied greatly in composition, structure, and physicochemical properties were selected for the analysis of structure-function relationships.
28325341	5	33	theme	gel	700:702	arg1	texture					704:710	gel texture	700:710	gel texture	700:710	The parameters of gel texture and amylose leaching are much related to amylopectin internal structure.
28325341	2	34	theme	Quinoa	185:190	arg1	starch					192:197	Quinoa starch	185:197	Quinoa starch	185:197	Quinoa starch has very small granules (∼2μm) with unique properties.
28325341	1	35	theme	components	135:144	arg1	relationships					111:123	Structure-function relationships	92:123	Structure-function relationships of starch components	92:144	Structure-function relationships of starch components remain a subject of research interest.
28325341	3	36	theme	relationships	416:428	arg1	analysis					385:392	the analysis	381:392	the analysis of structure-function relationships	381:428	In this study, nine quinoa starches varied greatly in composition, structure, and physicochemical properties were selected for the analysis of structure-function relationships.
28325341	5	37	theme	texture	704:710	arg1	parameters					686:695	The parameters	682:695	The parameters of gel texture and amylose leaching	682:731	The parameters of gel texture and amylose leaching are much related to amylopectin internal structure.
28325341	5	37	theme	texture	704:710	arg1	related					742:748	related	742:748	related	742:748	The parameters of gel texture and amylose leaching are much related to amylopectin internal structure.
28325341	7	38	theme	structure	1012:1020	arg1	importance					977:986	the importance	973:986	the importance of amylopectin internal structure and individual unit chain profile in determining the physicochemical properties of starch	973:1110	Interesting findings indicate the importance of amylopectin internal structure and individual unit chain profile in determining the physicochemical properties of starch.
28325341	6	39	theme	Other	785:789	arg1	susceptibility					817:830	enzyme susceptibility	810:830	enzyme susceptibility	810:830	Other properties such as enzyme susceptibility and particle size distribution are also strongly correlated with starch composition and amylopectin structure.
28325341	6	39	theme	Other	785:789	arg1	distribution					850:861	particle size distribution	836:861	particle size distribution	836:861	Other properties such as enzyme susceptibility and particle size distribution are also strongly correlated with starch composition and amylopectin structure.
28325341	6	39	theme	Other	785:789	arg1	properties					791:800	Other properties	785:800	Other properties such as enzyme susceptibility and particle size distribution	785:861	Other properties such as enzyme susceptibility and particle size distribution are also strongly correlated with starch composition and amylopectin structure.
28325341	7	40	theme	internal	1003:1010	arg1	structure					1012:1020	amylopectin internal structure	991:1020	amylopectin internal structure	991:1020	Interesting findings indicate the importance of amylopectin internal structure and individual unit chain profile in determining the physicochemical properties of starch.
28325341	8	41	theme	starch	1235:1240	arg1	composition					1159:1169	composition	1159:1169	composition	1159:1169	This work highlights some relationships among composition, amylopectin structure and physicochemical properties of quinoa starch.
28325341	8	41	theme	starch	1235:1240	arg1	structure					1184:1192	amylopectin structure	1172:1192	amylopectin structure	1172:1192	This work highlights some relationships among composition, amylopectin structure and physicochemical properties of quinoa starch.
28325341	8	41	theme	starch	1235:1240	arg1	properties					1214:1223	physicochemical properties	1198:1223	physicochemical properties	1198:1223	This work highlights some relationships among composition, amylopectin structure and physicochemical properties of quinoa starch.
28325341	4	42	theme	correlation	439:449	arg1	analysis					451:458	Pearson correlation analysis	431:458	Pearson correlation analysis	431:458	Pearson correlation analysis revealed that the properties related to gelatinization such as swelling power, water solubility index, crystallinity, pasting, and thermal properties are much affected by the amylopectin chain profile and amylose content.
28325341	7	43	theme	profile	1048:1054	arg1	importance					977:986	the importance	973:986	the importance of amylopectin internal structure and individual unit chain profile in determining the physicochemical properties of starch	973:1110	Interesting findings indicate the importance of amylopectin internal structure and individual unit chain profile in determining the physicochemical properties of starch.
28325341	5	44	theme	amylose	716:722	arg1	leaching					724:731	amylose leaching	716:731	amylose leaching	716:731	The parameters of gel texture and amylose leaching are much related to amylopectin internal structure.
28325341	0	45	theme	quinoa	77:82	arg1	starch					84:89	quinoa starch	77:89	quinoa starch	77:89	Amylopectin molecular structure in relation to physicochemical properties of quinoa starch.
28325341	2	46	theme	small	208:212	arg1	granules					214:221	very small granules	203:221	very small granules (∼2μm)	203:228	Quinoa starch has very small granules (∼2μm) with unique properties.
28325341	2	46	theme	small	208:212	arg1	∼2μm					224:227	∼2μm	224:227	∼2μm	224:227	Quinoa starch has very small granules (∼2μm) with unique properties.
28325341	7	47	theme	physicochemical	1075:1089	arg1	properties					1091:1100	the physicochemical properties	1071:1100	the physicochemical properties of starch	1071:1110	Interesting findings indicate the importance of amylopectin internal structure and individual unit chain profile in determining the physicochemical properties of starch.
28325341	4	48	theme	chain	647:651	arg1	profile					653:659	the amylopectin chain profile	631:659	the amylopectin chain profile	631:659	Pearson correlation analysis revealed that the properties related to gelatinization such as swelling power, water solubility index, crystallinity, pasting, and thermal properties are much affected by the amylopectin chain profile and amylose content.
28325341	5	49	theme	leaching	724:731	arg1	parameters					686:695	The parameters	682:695	The parameters of gel texture and amylose leaching	682:731	The parameters of gel texture and amylose leaching are much related to amylopectin internal structure.
28325341	5	49	theme	leaching	724:731	arg1	related					742:748	related	742:748	related	742:748	The parameters of gel texture and amylose leaching are much related to amylopectin internal structure.
28325341	1	50	theme	research	166:173	arg1	interest					175:182	research interest	166:182	research interest	166:182	Structure-function relationships of starch components remain a subject of research interest.
28325341	7	51	theme	Interesting	943:953	arg1	findings					955:962	Interesting findings	943:962	Interesting findings	943:962	Interesting findings indicate the importance of amylopectin internal structure and individual unit chain profile in determining the physicochemical properties of starch.
28325341	4	52	theme	amylopectin	635:645	arg1	profile					653:659	the amylopectin chain profile	631:659	the amylopectin chain profile	631:659	Pearson correlation analysis revealed that the properties related to gelatinization such as swelling power, water solubility index, crystallinity, pasting, and thermal properties are much affected by the amylopectin chain profile and amylose content.
28640580	2	0	theme	chronic	603:609	arg1	healing					617:623	chronic wound healing	603:623	chronic wound healing	603:623	Inspired by a naturally occurring hydrogel secreted from the carnivorous Sundew plant for trapping insects, here we have developed a bioinspired hydrogel to deliver mitsugumin 53 (MG53), an important protein in cell membrane repair, for chronic wound healing.
28640580	1	1	theme	bioactive	281:289	arg1	microenvironment					300:315	bioactive cellular microenvironment	281:315	bioactive cellular microenvironment	281:315	Biopolymeric hydrogels have drawn increasing research interest in biomaterials due to their tunable physical and chemical properties for both creating bioactive cellular microenvironment and serving as sustainable therapeutic reagents.
28640580	7	2	theme	protein	1582:1588	arg1	release					1567:1573	sustained release	1557:1573	sustained release of the protein	1557:1588	In vitro drug release experiments confirmed that the Sundew-inspired hydrogel had a biphasic-kinetics release, which can facilitate both fast delivery of MG53 for improving the reepithelization process of the wounds and sustained release of the protein for treating chronic wounds.
28640580	7	2	theme	protein	1582:1588	arg1	delivery					1479:1486	fast delivery	1474:1486	fast delivery of MG53 for improving the reepithelization process of the wounds	1474:1551	In vitro drug release experiments confirmed that the Sundew-inspired hydrogel had a biphasic-kinetics release, which can facilitate both fast delivery of MG53 for improving the reepithelization process of the wounds and sustained release of the protein for treating chronic wounds.
28640580	3	3	theme	arabic	779:784	arg1	alginate					762:769	sodium alginate	755:769	sodium alginate	755:769	Both chemical compositions and micro-/nanomorphological properties inherent from the natural Sundew hydrogel were mimicked using sodium alginate and gum arabic with calcium ion-mediated cross-linking.
28640580	9	4	contain	has	1828:1830	arg2	properties					1887:1896	physicochemical properties	1871:1896	physicochemical properties	1871:1896	Together, these studies confirmed that the Sundew-inspired hydrogel has both tunable micro-/nanostructures and physicochemical properties, which enable it as a delivery vehicle for chronic wounding healing.
28640580	9	4	contain	has	1828:1830	arg2	vehicle					1929:1935	a delivery vehicle	1918:1935	a delivery vehicle for chronic wounding healing	1918:1964	Together, these studies confirmed that the Sundew-inspired hydrogel has both tunable micro-/nanostructures and physicochemical properties, which enable it as a delivery vehicle for chronic wounding healing.
28640580	9	4	contain	has	1828:1830	arg1	hydrogel					1819:1826	the Sundew-inspired hydrogel	1799:1826	the Sundew-inspired hydrogel	1799:1826	Together, these studies confirmed that the Sundew-inspired hydrogel has both tunable micro-/nanostructures and physicochemical properties, which enable it as a delivery vehicle for chronic wounding healing.
28640580	9	4	contain	has	1828:1830	arg2	micro-/nanostructures					1845:1865	tunable micro-/nanostructures	1837:1865	tunable micro-/nanostructures	1837:1865	Together, these studies confirmed that the Sundew-inspired hydrogel has both tunable micro-/nanostructures and physicochemical properties, which enable it as a delivery vehicle for chronic wounding healing.
28640580	7	5	theme	chronic	1603:1609	arg1	wounds					1611:1616	chronic wounds	1603:1616	chronic wounds	1603:1616	In vitro drug release experiments confirmed that the Sundew-inspired hydrogel had a biphasic-kinetics release, which can facilitate both fast delivery of MG53 for improving the reepithelization process of the wounds and sustained release of the protein for treating chronic wounds.
28640580	4	6	theme	sticky	904:909	arg1	scaffold					920:927	an optimal sticky hydrogel scaffold	893:927	an optimal sticky hydrogel scaffold	893:927	On the basis of atomic force microscopy (AFM) force measurements, an optimal sticky hydrogel scaffold was obtained through orthogonal experimental design.
28640580	7	7	theme	sustained	1557:1565	arg1	release					1567:1573	sustained release	1557:1573	sustained release of the protein	1557:1588	In vitro drug release experiments confirmed that the Sundew-inspired hydrogel had a biphasic-kinetics release, which can facilitate both fast delivery of MG53 for improving the reepithelization process of the wounds and sustained release of the protein for treating chronic wounds.
28640580	2	8	theme	membrane	582:589	arg1	repair					591:596	cell membrane repair	577:596	cell membrane repair	577:596	Inspired by a naturally occurring hydrogel secreted from the carnivorous Sundew plant for trapping insects, here we have developed a bioinspired hydrogel to deliver mitsugumin 53 (MG53), an important protein in cell membrane repair, for chronic wound healing.
28640580	7	9	theme	wounds	1546:1551	arg1	process					1531:1537	the reepithelization process	1510:1537	the reepithelization process of the wounds	1510:1551	In vitro drug release experiments confirmed that the Sundew-inspired hydrogel had a biphasic-kinetics release, which can facilitate both fast delivery of MG53 for improving the reepithelization process of the wounds and sustained release of the protein for treating chronic wounds.
28640580	3	10	theme	calcium	791:797	arg1	cross-linking					812:824	calcium ion-mediated cross-linking	791:824	calcium ion-mediated cross-linking	791:824	Both chemical compositions and micro-/nanomorphological properties inherent from the natural Sundew hydrogel were mimicked using sodium alginate and gum arabic with calcium ion-mediated cross-linking.
28640580	1	11	theme	tunable	222:228	arg1	properties					252:261	their tunable physical and chemical properties	216:261	their tunable physical and chemical properties for both creating bioactive cellular microenvironment and serving as sustainable therapeutic reagents	216:363	Biopolymeric hydrogels have drawn increasing research interest in biomaterials due to their tunable physical and chemical properties for both creating bioactive cellular microenvironment and serving as sustainable therapeutic reagents.
28640580	7	12	theme	fast	1474:1477	arg1	delivery					1479:1486	fast delivery	1474:1486	fast delivery of MG53 for improving the reepithelization process of the wounds	1474:1551	In vitro drug release experiments confirmed that the Sundew-inspired hydrogel had a biphasic-kinetics release, which can facilitate both fast delivery of MG53 for improving the reepithelization process of the wounds and sustained release of the protein for treating chronic wounds.
28640580	10	13	theme	new	1994:1996	arg1	way					1998:2000	a new way	1992:2000	a new way to develop biocompatible and tunable biomaterials for sustainable drug release to meet the needs of biological activities	1992:2122	The research may provide a new way to develop biocompatible and tunable biomaterials for sustainable drug release to meet the needs of biological activities.
28640580	4	14	theme	orthogonal	950:959	arg1	design					974:979	orthogonal experimental design	950:979	orthogonal experimental design	950:979	On the basis of atomic force microscopy (AFM) force measurements, an optimal sticky hydrogel scaffold was obtained through orthogonal experimental design.
28640580	7	15	theme	reepithelization	1514:1529	arg1	process					1531:1537	the reepithelization process	1510:1537	the reepithelization process of the wounds	1510:1551	In vitro drug release experiments confirmed that the Sundew-inspired hydrogel had a biphasic-kinetics release, which can facilitate both fast delivery of MG53 for improving the reepithelization process of the wounds and sustained release of the protein for treating chronic wounds.
28640580	6	16	used	utilized	1221:1228	arg2	techniques					1205:1214	Combined characterization and biochemistry techniques	1162:1214	Combined characterization and biochemistry techniques	1162:1214	Combined characterization and biochemistry techniques were utilized to uncover the underlying molecular composition involved in the interactions between hydrogel and protein.
28640580	1	17	theme	Biopolymeric	130:141	arg1	hydrogels					143:151	Biopolymeric hydrogels	130:151	Biopolymeric hydrogels	130:151	Biopolymeric hydrogels have drawn increasing research interest in biomaterials due to their tunable physical and chemical properties for both creating bioactive cellular microenvironment and serving as sustainable therapeutic reagents.
28640580	9	18	theme	delivery	1920:1927	arg1	properties					1887:1896	physicochemical properties	1871:1896	physicochemical properties	1871:1896	Together, these studies confirmed that the Sundew-inspired hydrogel has both tunable micro-/nanostructures and physicochemical properties, which enable it as a delivery vehicle for chronic wounding healing.
28640580	9	18	theme	delivery	1920:1927	arg1	micro-/nanostructures					1845:1865	tunable micro-/nanostructures	1837:1865	tunable micro-/nanostructures	1837:1865	Together, these studies confirmed that the Sundew-inspired hydrogel has both tunable micro-/nanostructures and physicochemical properties, which enable it as a delivery vehicle for chronic wounding healing.
28640580	9	18	theme	delivery	1920:1927	arg1	vehicle					1929:1935	a delivery vehicle	1918:1935	a delivery vehicle for chronic wounding healing	1918:1964	Together, these studies confirmed that the Sundew-inspired hydrogel has both tunable micro-/nanostructures and physicochemical properties, which enable it as a delivery vehicle for chronic wounding healing.
28640580	8	19	theme	In	1619:1620	arg1	experiments					1627:1637	In vivo experiments	1619:1637	In vivo experiments	1619:1637	In vivo experiments showed that the Sundew-inspired hydrogel encapsulating with rhMG53 could facilitate dermal wound healing in mouse model.
28640580	5	20	theme	hydrogel	1152:1159	arg1	characteristics					1086:1100	adhesion characteristics	1077:1100	adhesion characteristics	1077:1100	Imaging and mechanical analysis showed the distinct correlation between structural morphology, adhesion characteristics, and mechanical properties of the Sundew-inspired hydrogel.
28640580	5	20	theme	hydrogel	1152:1159	arg1	properties					1118:1127	mechanical properties	1107:1127	mechanical properties of the Sundew-inspired hydrogel	1107:1159	Imaging and mechanical analysis showed the distinct correlation between structural morphology, adhesion characteristics, and mechanical properties of the Sundew-inspired hydrogel.
28640580	5	20	theme	hydrogel	1152:1159	arg1	morphology					1065:1074	structural morphology	1054:1074	structural morphology	1054:1074	Imaging and mechanical analysis showed the distinct correlation between structural morphology, adhesion characteristics, and mechanical properties of the Sundew-inspired hydrogel.
28640580	3	21	theme	micro-/nanomorphological	657:680	arg1	properties					682:691	micro-/nanomorphological properties	657:691	micro-/nanomorphological properties	657:691	Both chemical compositions and micro-/nanomorphological properties inherent from the natural Sundew hydrogel were mimicked using sodium alginate and gum arabic with calcium ion-mediated cross-linking.
28640580	5	22	theme	mechanical	994:1003	arg1	analysis					1005:1012	mechanical analysis	994:1012	mechanical analysis	994:1012	Imaging and mechanical analysis showed the distinct correlation between structural morphology, adhesion characteristics, and mechanical properties of the Sundew-inspired hydrogel.
28640580	7	23	theme	MG53	1491:1494	arg1	release					1567:1573	sustained release	1557:1573	sustained release of the protein	1557:1588	In vitro drug release experiments confirmed that the Sundew-inspired hydrogel had a biphasic-kinetics release, which can facilitate both fast delivery of MG53 for improving the reepithelization process of the wounds and sustained release of the protein for treating chronic wounds.
28640580	7	23	theme	MG53	1491:1494	arg1	delivery					1479:1486	fast delivery	1474:1486	fast delivery of MG53 for improving the reepithelization process of the wounds	1474:1551	In vitro drug release experiments confirmed that the Sundew-inspired hydrogel had a biphasic-kinetics release, which can facilitate both fast delivery of MG53 for improving the reepithelization process of the wounds and sustained release of the protein for treating chronic wounds.
28640580	1	24	theme	increasing	164:173	arg1	interest					184:191	increasing research interest	164:191	increasing research interest in biomaterials	164:207	Biopolymeric hydrogels have drawn increasing research interest in biomaterials due to their tunable physical and chemical properties for both creating bioactive cellular microenvironment and serving as sustainable therapeutic reagents.
28640580	9	25	theme	wounding	1949:1956	arg1	healing					1958:1964	chronic wounding healing	1941:1964	chronic wounding healing	1941:1964	Together, these studies confirmed that the Sundew-inspired hydrogel has both tunable micro-/nanostructures and physicochemical properties, which enable it as a delivery vehicle for chronic wounding healing.
28640580	0	26	theme	Drug	91:94	arg1	Release					96:102	Controlled Drug Release	80:102	Controlled Drug Release in Chronic Wound Healing	80:127	A Bioinspired Alginate-Gum Arabic Hydrogel with Micro-/Nanoscale Structures for Controlled Drug Release in Chronic Wound Healing.
28640580	9	27	theme	tunable	1837:1843	arg1	properties					1887:1896	physicochemical properties	1871:1896	physicochemical properties	1871:1896	Together, these studies confirmed that the Sundew-inspired hydrogel has both tunable micro-/nanostructures and physicochemical properties, which enable it as a delivery vehicle for chronic wounding healing.
28640580	9	27	theme	tunable	1837:1843	arg1	micro-/nanostructures					1845:1865	tunable micro-/nanostructures	1837:1865	tunable micro-/nanostructures	1837:1865	Together, these studies confirmed that the Sundew-inspired hydrogel has both tunable micro-/nanostructures and physicochemical properties, which enable it as a delivery vehicle for chronic wounding healing.
28640580	9	27	theme	tunable	1837:1843	arg1	vehicle					1929:1935	a delivery vehicle	1918:1935	a delivery vehicle for chronic wounding healing	1918:1964	Together, these studies confirmed that the Sundew-inspired hydrogel has both tunable micro-/nanostructures and physicochemical properties, which enable it as a delivery vehicle for chronic wounding healing.
28640580	2	28	theme	important	556:564	arg1	protein					566:572	an important protein	553:572	an important protein in cell membrane repair	553:596	Inspired by a naturally occurring hydrogel secreted from the carnivorous Sundew plant for trapping insects, here we have developed a bioinspired hydrogel to deliver mitsugumin 53 (MG53), an important protein in cell membrane repair, for chronic wound healing.
28640580	2	28	theme	important	556:564	arg1	mitsugumin					531:540	mitsugumin 53	531:543	mitsugumin 53 (MG53)	531:550	Inspired by a naturally occurring hydrogel secreted from the carnivorous Sundew plant for trapping insects, here we have developed a bioinspired hydrogel to deliver mitsugumin 53 (MG53), an important protein in cell membrane repair, for chronic wound healing.
28640580	8	29	theme	Sundew-inspired	1655:1669	arg1	hydrogel					1671:1678	the Sundew-inspired hydrogel	1651:1678	the Sundew-inspired hydrogel encapsulating with rhMG53	1651:1704	In vivo experiments showed that the Sundew-inspired hydrogel encapsulating with rhMG53 could facilitate dermal wound healing in mouse model.
28640580	0	30	theme	Wound	115:119	arg1	Healing					121:127	Chronic Wound Healing	107:127	Chronic Wound Healing	107:127	A Bioinspired Alginate-Gum Arabic Hydrogel with Micro-/Nanoscale Structures for Controlled Drug Release in Chronic Wound Healing.
28640580	1	31	theme	sustainable	332:342	arg1	reagents					356:363	sustainable therapeutic reagents	332:363	sustainable therapeutic reagents	332:363	Biopolymeric hydrogels have drawn increasing research interest in biomaterials due to their tunable physical and chemical properties for both creating bioactive cellular microenvironment and serving as sustainable therapeutic reagents.
28640580	9	32	theme	physicochemical	1871:1885	arg1	vehicle					1929:1935	a delivery vehicle	1918:1935	a delivery vehicle for chronic wounding healing	1918:1964	Together, these studies confirmed that the Sundew-inspired hydrogel has both tunable micro-/nanostructures and physicochemical properties, which enable it as a delivery vehicle for chronic wounding healing.
28640580	9	32	theme	physicochemical	1871:1885	arg1	micro-/nanostructures					1845:1865	tunable micro-/nanostructures	1837:1865	tunable micro-/nanostructures	1837:1865	Together, these studies confirmed that the Sundew-inspired hydrogel has both tunable micro-/nanostructures and physicochemical properties, which enable it as a delivery vehicle for chronic wounding healing.
28640580	9	32	theme	physicochemical	1871:1885	arg1	properties					1887:1896	physicochemical properties	1871:1896	physicochemical properties	1871:1896	Together, these studies confirmed that the Sundew-inspired hydrogel has both tunable micro-/nanostructures and physicochemical properties, which enable it as a delivery vehicle for chronic wounding healing.
28640580	2	33	theme	Sundew	439:444	arg1	plant					446:450	the carnivorous Sundew plant	423:450	the carnivorous Sundew plant for trapping insects	423:471	Inspired by a naturally occurring hydrogel secreted from the carnivorous Sundew plant for trapping insects, here we have developed a bioinspired hydrogel to deliver mitsugumin 53 (MG53), an important protein in cell membrane repair, for chronic wound healing.
28640580	6	34	theme	Combined	1162:1169	arg1	techniques					1205:1214	Combined characterization and biochemistry techniques	1162:1214	Combined characterization and biochemistry techniques	1162:1214	Combined characterization and biochemistry techniques were utilized to uncover the underlying molecular composition involved in the interactions between hydrogel and protein.
28640580	0	35	theme	Alginate-Gum	14:25	arg1	Hydrogel					34:41	A Bioinspired Alginate-Gum Arabic Hydrogel	0:41	A Bioinspired Alginate-Gum Arabic Hydrogel with Micro-/Nanoscale Structures for Controlled Drug Release in Chronic Wound Healing.	0:128	A Bioinspired Alginate-Gum Arabic Hydrogel with Micro-/Nanoscale Structures for Controlled Drug Release in Chronic Wound Healing.
28640580	5	36	theme	distinct	1025:1032	arg1	correlation					1034:1044	the distinct correlation	1021:1044	the distinct correlation between structural morphology, adhesion characteristics, and mechanical properties of the Sundew-inspired hydrogel	1021:1159	Imaging and mechanical analysis showed the distinct correlation between structural morphology, adhesion characteristics, and mechanical properties of the Sundew-inspired hydrogel.
28640580	8	37	theme	wound	1730:1734	arg1	healing					1736:1742	dermal wound healing	1723:1742	dermal wound healing	1723:1742	In vivo experiments showed that the Sundew-inspired hydrogel encapsulating with rhMG53 could facilitate dermal wound healing in mouse model.
28640580	1	38	theme	therapeutic	344:354	arg1	reagents					356:363	sustainable therapeutic reagents	332:363	sustainable therapeutic reagents	332:363	Biopolymeric hydrogels have drawn increasing research interest in biomaterials due to their tunable physical and chemical properties for both creating bioactive cellular microenvironment and serving as sustainable therapeutic reagents.
28640580	5	39	theme	mechanical	1107:1116	arg1	properties					1118:1127	mechanical properties	1107:1127	mechanical properties of the Sundew-inspired hydrogel	1107:1159	Imaging and mechanical analysis showed the distinct correlation between structural morphology, adhesion characteristics, and mechanical properties of the Sundew-inspired hydrogel.
28640580	10	40	dep	the	2089:2091	arg1	needs					2093:2097	needs	2093:2097	needs	2093:2097	The research may provide a new way to develop biocompatible and tunable biomaterials for sustainable drug release to meet the needs of biological activities.
28640580	3	41	theme	inherent	693:700	arg1	compositions					640:651	chemical compositions	631:651	chemical compositions	631:651	Both chemical compositions and micro-/nanomorphological properties inherent from the natural Sundew hydrogel were mimicked using sodium alginate and gum arabic with calcium ion-mediated cross-linking.
28640580	2	42	theme	occurring	390:398	arg1	hydrogel					400:407	a naturally occurring hydrogel	378:407	a naturally occurring hydrogel secreted from the carnivorous Sundew plant for trapping insects	378:471	Inspired by a naturally occurring hydrogel secreted from the carnivorous Sundew plant for trapping insects, here we have developed a bioinspired hydrogel to deliver mitsugumin 53 (MG53), an important protein in cell membrane repair, for chronic wound healing.
28640580	0	43	theme	Micro-/Nanoscale	48:63	arg1	Structures					65:74	Micro-/Nanoscale Structures	48:74	Micro-/Nanoscale Structures	48:74	A Bioinspired Alginate-Gum Arabic Hydrogel with Micro-/Nanoscale Structures for Controlled Drug Release in Chronic Wound Healing.
28640580	6	44	theme	underlying	1245:1254	arg1	composition					1266:1276	the underlying molecular composition	1241:1276	the underlying molecular composition involved in the interactions between hydrogel and protein	1241:1334	Combined characterization and biochemistry techniques were utilized to uncover the underlying molecular composition involved in the interactions between hydrogel and protein.
28640580	7	45	dep	In	1337:1338	arg1	vitro					1340:1344	vitro	1340:1344	vitro	1340:1344	In vitro drug release experiments confirmed that the Sundew-inspired hydrogel had a biphasic-kinetics release, which can facilitate both fast delivery of MG53 for improving the reepithelization process of the wounds and sustained release of the protein for treating chronic wounds.
28640580	4	46	theme	force	850:854	arg1	microscopy					856:865	atomic force microscopy	843:865	atomic force microscopy (AFM) force measurements	843:890	On the basis of atomic force microscopy (AFM) force measurements, an optimal sticky hydrogel scaffold was obtained through orthogonal experimental design.
28640580	4	46	theme	force	850:854	arg1	AFM					868:870	AFM	868:870	AFM	868:870	On the basis of atomic force microscopy (AFM) force measurements, an optimal sticky hydrogel scaffold was obtained through orthogonal experimental design.
28640580	1	47	theme	chemical	243:250	arg1	properties					252:261	their tunable physical and chemical properties	216:261	their tunable physical and chemical properties for both creating bioactive cellular microenvironment and serving as sustainable therapeutic reagents	216:363	Biopolymeric hydrogels have drawn increasing research interest in biomaterials due to their tunable physical and chemical properties for both creating bioactive cellular microenvironment and serving as sustainable therapeutic reagents.
28640580	5	48	theme	adhesion	1077:1084	arg1	characteristics					1086:1100	adhesion characteristics	1077:1100	adhesion characteristics	1077:1100	Imaging and mechanical analysis showed the distinct correlation between structural morphology, adhesion characteristics, and mechanical properties of the Sundew-inspired hydrogel.
28640580	2	49	from	protein	566:572	arg1	repair					591:596	cell membrane repair	577:596	cell membrane repair	577:596	Inspired by a naturally occurring hydrogel secreted from the carnivorous Sundew plant for trapping insects, here we have developed a bioinspired hydrogel to deliver mitsugumin 53 (MG53), an important protein in cell membrane repair, for chronic wound healing.
28640580	3	50	theme	Sundew	719:724	arg1	hydrogel					726:733	the natural Sundew hydrogel	707:733	the natural Sundew hydrogel	707:733	Both chemical compositions and micro-/nanomorphological properties inherent from the natural Sundew hydrogel were mimicked using sodium alginate and gum arabic with calcium ion-mediated cross-linking.
28640580	9	51	theme	Sundew-inspired	1803:1817	arg1	hydrogel					1819:1826	the Sundew-inspired hydrogel	1799:1826	the Sundew-inspired hydrogel	1799:1826	Together, these studies confirmed that the Sundew-inspired hydrogel has both tunable micro-/nanostructures and physicochemical properties, which enable it as a delivery vehicle for chronic wounding healing.
28640580	10	52	theme	biocompatible	2013:2025	arg1	biomaterials					2039:2050	biocompatible and tunable biomaterials	2013:2050	biocompatible and tunable biomaterials for sustainable drug release	2013:2079	The research may provide a new way to develop biocompatible and tunable biomaterials for sustainable drug release to meet the needs of biological activities.
28640580	3	53	from	hydrogel	726:733	arg1	inherent					693:700	inherent	693:700	inherent	693:700	Both chemical compositions and micro-/nanomorphological properties inherent from the natural Sundew hydrogel were mimicked using sodium alginate and gum arabic with calcium ion-mediated cross-linking.
28640580	0	54	with	Hydrogel	34:41	arg1	Structures					65:74	Micro-/Nanoscale Structures	48:74	Micro-/Nanoscale Structures	48:74	A Bioinspired Alginate-Gum Arabic Hydrogel with Micro-/Nanoscale Structures for Controlled Drug Release in Chronic Wound Healing.
28640580	4	55	theme	hydrogel	911:918	arg1	scaffold					920:927	an optimal sticky hydrogel scaffold	893:927	an optimal sticky hydrogel scaffold	893:927	On the basis of atomic force microscopy (AFM) force measurements, an optimal sticky hydrogel scaffold was obtained through orthogonal experimental design.
28640580	10	56	theme	tunable	2031:2037	arg1	biomaterials					2039:2050	biocompatible and tunable biomaterials	2013:2050	biocompatible and tunable biomaterials for sustainable drug release	2013:2079	The research may provide a new way to develop biocompatible and tunable biomaterials for sustainable drug release to meet the needs of biological activities.
28640580	1	57	theme	cellular	291:298	arg1	microenvironment					300:315	bioactive cellular microenvironment	281:315	bioactive cellular microenvironment	281:315	Biopolymeric hydrogels have drawn increasing research interest in biomaterials due to their tunable physical and chemical properties for both creating bioactive cellular microenvironment and serving as sustainable therapeutic reagents.
28640580	2	58	theme	wound	611:615	arg1	healing					617:623	chronic wound healing	603:623	chronic wound healing	603:623	Inspired by a naturally occurring hydrogel secreted from the carnivorous Sundew plant for trapping insects, here we have developed a bioinspired hydrogel to deliver mitsugumin 53 (MG53), an important protein in cell membrane repair, for chronic wound healing.
28640580	6	59	theme	molecular	1256:1264	arg1	composition					1266:1276	the underlying molecular composition	1241:1276	the underlying molecular composition involved in the interactions between hydrogel and protein	1241:1334	Combined characterization and biochemistry techniques were utilized to uncover the underlying molecular composition involved in the interactions between hydrogel and protein.
28640580	7	60	theme	release	1351:1357	arg1	experiments					1359:1369	In vitro drug release experiments	1337:1369	In vitro drug release experiments	1337:1369	In vitro drug release experiments confirmed that the Sundew-inspired hydrogel had a biphasic-kinetics release, which can facilitate both fast delivery of MG53 for improving the reepithelization process of the wounds and sustained release of the protein for treating chronic wounds.
28640580	4	61	theme	optimal	896:902	arg1	scaffold					920:927	an optimal sticky hydrogel scaffold	893:927	an optimal sticky hydrogel scaffold	893:927	On the basis of atomic force microscopy (AFM) force measurements, an optimal sticky hydrogel scaffold was obtained through orthogonal experimental design.
28640580	4	62	dep	measurements	879:890	arg1	the					830:832	the	830:832	the	830:832	On the basis of atomic force microscopy (AFM) force measurements, an optimal sticky hydrogel scaffold was obtained through orthogonal experimental design.
28640580	4	62	dep	measurements	879:890	arg1	basis					834:838	basis	834:838	basis	834:838	On the basis of atomic force microscopy (AFM) force measurements, an optimal sticky hydrogel scaffold was obtained through orthogonal experimental design.
28640580	2	63	theme	cell	577:580	arg1	repair					591:596	cell membrane repair	577:596	cell membrane repair	577:596	Inspired by a naturally occurring hydrogel secreted from the carnivorous Sundew plant for trapping insects, here we have developed a bioinspired hydrogel to deliver mitsugumin 53 (MG53), an important protein in cell membrane repair, for chronic wound healing.
28640580	0	64	from	Release	96:102	arg1	Healing					121:127	Chronic Wound Healing	107:127	Chronic Wound Healing	107:127	A Bioinspired Alginate-Gum Arabic Hydrogel with Micro-/Nanoscale Structures for Controlled Drug Release in Chronic Wound Healing.
28640580	3	65	theme	ion-mediated	799:810	arg1	cross-linking					812:824	calcium ion-mediated cross-linking	791:824	calcium ion-mediated cross-linking	791:824	Both chemical compositions and micro-/nanomorphological properties inherent from the natural Sundew hydrogel were mimicked using sodium alginate and gum arabic with calcium ion-mediated cross-linking.
28640580	1	66	theme	physical	230:237	arg1	properties					252:261	their tunable physical and chemical properties	216:261	their tunable physical and chemical properties for both creating bioactive cellular microenvironment and serving as sustainable therapeutic reagents	216:363	Biopolymeric hydrogels have drawn increasing research interest in biomaterials due to their tunable physical and chemical properties for both creating bioactive cellular microenvironment and serving as sustainable therapeutic reagents.
28640580	7	67	theme	In	1337:1338	arg1	experiments					1359:1369	In vitro drug release experiments	1337:1369	In vitro drug release experiments	1337:1369	In vitro drug release experiments confirmed that the Sundew-inspired hydrogel had a biphasic-kinetics release, which can facilitate both fast delivery of MG53 for improving the reepithelization process of the wounds and sustained release of the protein for treating chronic wounds.
28640580	5	68	theme	Sundew-inspired	1136:1150	arg1	hydrogel					1152:1159	the Sundew-inspired hydrogel	1132:1159	the Sundew-inspired hydrogel	1132:1159	Imaging and mechanical analysis showed the distinct correlation between structural morphology, adhesion characteristics, and mechanical properties of the Sundew-inspired hydrogel.
28640580	4	69	theme	experimental	961:972	arg1	design					974:979	orthogonal experimental design	950:979	orthogonal experimental design	950:979	On the basis of atomic force microscopy (AFM) force measurements, an optimal sticky hydrogel scaffold was obtained through orthogonal experimental design.
28640580	0	70	theme	Controlled	80:89	arg1	Release					96:102	Controlled Drug Release	80:102	Controlled Drug Release in Chronic Wound Healing	80:127	A Bioinspired Alginate-Gum Arabic Hydrogel with Micro-/Nanoscale Structures for Controlled Drug Release in Chronic Wound Healing.
28640580	3	71	theme	chemical	631:638	arg1	compositions					640:651	chemical compositions	631:651	chemical compositions	631:651	Both chemical compositions and micro-/nanomorphological properties inherent from the natural Sundew hydrogel were mimicked using sodium alginate and gum arabic with calcium ion-mediated cross-linking.
28640580	9	72	theme	chronic	1941:1947	arg1	healing					1958:1964	chronic wounding healing	1941:1964	chronic wounding healing	1941:1964	Together, these studies confirmed that the Sundew-inspired hydrogel has both tunable micro-/nanostructures and physicochemical properties, which enable it as a delivery vehicle for chronic wounding healing.
28640580	7	73	theme	drug	1346:1349	arg1	experiments					1359:1369	In vitro drug release experiments	1337:1369	In vitro drug release experiments	1337:1369	In vitro drug release experiments confirmed that the Sundew-inspired hydrogel had a biphasic-kinetics release, which can facilitate both fast delivery of MG53 for improving the reepithelization process of the wounds and sustained release of the protein for treating chronic wounds.
28640580	1	74	theme	research	175:182	arg1	interest					184:191	increasing research interest	164:191	increasing research interest in biomaterials	164:207	Biopolymeric hydrogels have drawn increasing research interest in biomaterials due to their tunable physical and chemical properties for both creating bioactive cellular microenvironment and serving as sustainable therapeutic reagents.
28640580	0	75	theme	Chronic	107:113	arg1	Healing					121:127	Chronic Wound Healing	107:127	Chronic Wound Healing	107:127	A Bioinspired Alginate-Gum Arabic Hydrogel with Micro-/Nanoscale Structures for Controlled Drug Release in Chronic Wound Healing.
28640580	1	76	from	interest	184:191	arg1	biomaterials					196:207	biomaterials	196:207	biomaterials	196:207	Biopolymeric hydrogels have drawn increasing research interest in biomaterials due to their tunable physical and chemical properties for both creating bioactive cellular microenvironment and serving as sustainable therapeutic reagents.
28640580	7	77	contain	had	1415:1417	arg2	release					1439:1445	a biphasic-kinetics release	1419:1445	a biphasic-kinetics release	1419:1445	In vitro drug release experiments confirmed that the Sundew-inspired hydrogel had a biphasic-kinetics release, which can facilitate both fast delivery of MG53 for improving the reepithelization process of the wounds and sustained release of the protein for treating chronic wounds.
28640580	7	77	contain	had	1415:1417	arg1	hydrogel					1406:1413	the Sundew-inspired hydrogel	1386:1413	the Sundew-inspired hydrogel	1386:1413	In vitro drug release experiments confirmed that the Sundew-inspired hydrogel had a biphasic-kinetics release, which can facilitate both fast delivery of MG53 for improving the reepithelization process of the wounds and sustained release of the protein for treating chronic wounds.
28640580	5	78	theme	structural	1054:1063	arg1	morphology					1065:1074	structural morphology	1054:1074	structural morphology	1054:1074	Imaging and mechanical analysis showed the distinct correlation between structural morphology, adhesion characteristics, and mechanical properties of the Sundew-inspired hydrogel.
28640580	6	79	theme	biochemistry	1192:1203	arg1	techniques					1205:1214	Combined characterization and biochemistry techniques	1162:1214	Combined characterization and biochemistry techniques	1162:1214	Combined characterization and biochemistry techniques were utilized to uncover the underlying molecular composition involved in the interactions between hydrogel and protein.
28640580	0	80	theme	Bioinspired	2:12	arg1	Hydrogel					34:41	A Bioinspired Alginate-Gum Arabic Hydrogel	0:41	A Bioinspired Alginate-Gum Arabic Hydrogel with Micro-/Nanoscale Structures for Controlled Drug Release in Chronic Wound Healing.	0:128	A Bioinspired Alginate-Gum Arabic Hydrogel with Micro-/Nanoscale Structures for Controlled Drug Release in Chronic Wound Healing.
28640580	6	81	theme	characterization	1171:1186	arg1	techniques					1205:1214	Combined characterization and biochemistry techniques	1162:1214	Combined characterization and biochemistry techniques	1162:1214	Combined characterization and biochemistry techniques were utilized to uncover the underlying molecular composition involved in the interactions between hydrogel and protein.
28640580	8	82	theme	dermal	1723:1728	arg1	healing					1736:1742	dermal wound healing	1723:1742	dermal wound healing	1723:1742	In vivo experiments showed that the Sundew-inspired hydrogel encapsulating with rhMG53 could facilitate dermal wound healing in mouse model.
28640580	10	83	theme	drug	2068:2071	arg1	release					2073:2079	sustainable drug release	2056:2079	sustainable drug release	2056:2079	The research may provide a new way to develop biocompatible and tunable biomaterials for sustainable drug release to meet the needs of biological activities.
28640580	2	84	theme	carnivorous	427:437	arg1	plant					446:450	the carnivorous Sundew plant	423:450	the carnivorous Sundew plant for trapping insects	423:471	Inspired by a naturally occurring hydrogel secreted from the carnivorous Sundew plant for trapping insects, here we have developed a bioinspired hydrogel to deliver mitsugumin 53 (MG53), an important protein in cell membrane repair, for chronic wound healing.
28640580	0	85	theme	Arabic	27:32	arg1	Hydrogel					34:41	A Bioinspired Alginate-Gum Arabic Hydrogel	0:41	A Bioinspired Alginate-Gum Arabic Hydrogel with Micro-/Nanoscale Structures for Controlled Drug Release in Chronic Wound Healing.	0:128	A Bioinspired Alginate-Gum Arabic Hydrogel with Micro-/Nanoscale Structures for Controlled Drug Release in Chronic Wound Healing.
28640580	10	86	theme	sustainable	2056:2066	arg1	release					2073:2079	sustainable drug release	2056:2079	sustainable drug release	2056:2079	The research may provide a new way to develop biocompatible and tunable biomaterials for sustainable drug release to meet the needs of biological activities.
28640580	8	87	dep	In	1619:1620	arg1	vivo					1622:1625	vivo	1622:1625	vivo	1622:1625	In vivo experiments showed that the Sundew-inspired hydrogel encapsulating with rhMG53 could facilitate dermal wound healing in mouse model.
28640580	8	88	theme	mouse	1747:1751	arg1	model					1753:1757	mouse model	1747:1757	mouse model	1747:1757	In vivo experiments showed that the Sundew-inspired hydrogel encapsulating with rhMG53 could facilitate dermal wound healing in mouse model.
28640580	2	89	theme	trapping	456:463	arg1	insects					465:471	trapping insects	456:471	trapping insects	456:471	Inspired by a naturally occurring hydrogel secreted from the carnivorous Sundew plant for trapping insects, here we have developed a bioinspired hydrogel to deliver mitsugumin 53 (MG53), an important protein in cell membrane repair, for chronic wound healing.
28640580	7	90	theme	biphasic-kinetics	1421:1437	arg1	release					1439:1445	a biphasic-kinetics release	1419:1445	a biphasic-kinetics release	1419:1445	In vitro drug release experiments confirmed that the Sundew-inspired hydrogel had a biphasic-kinetics release, which can facilitate both fast delivery of MG53 for improving the reepithelization process of the wounds and sustained release of the protein for treating chronic wounds.
28640580	2	91	theme	bioinspired	499:509	arg1	hydrogel					511:518	a bioinspired hydrogel	497:518	a bioinspired hydrogel to deliver mitsugumin 53 (MG53), an important protein in cell membrane repair, for chronic wound healing	497:623	Inspired by a naturally occurring hydrogel secreted from the carnivorous Sundew plant for trapping insects, here we have developed a bioinspired hydrogel to deliver mitsugumin 53 (MG53), an important protein in cell membrane repair, for chronic wound healing.
28640580	10	92	theme	biological	2102:2111	arg1	activities					2113:2122	biological activities	2102:2122	biological activities	2102:2122	The research may provide a new way to develop biocompatible and tunable biomaterials for sustainable drug release to meet the needs of biological activities.
28640580	4	93	theme	force	873:877	arg1	measurements					879:890	atomic force microscopy (AFM) force measurements	843:890	atomic force microscopy (AFM) force measurements	843:890	On the basis of atomic force microscopy (AFM) force measurements, an optimal sticky hydrogel scaffold was obtained through orthogonal experimental design.
28640580	3	94	theme	natural	711:717	arg1	hydrogel					726:733	the natural Sundew hydrogel	707:733	the natural Sundew hydrogel	707:733	Both chemical compositions and micro-/nanomorphological properties inherent from the natural Sundew hydrogel were mimicked using sodium alginate and gum arabic with calcium ion-mediated cross-linking.
28640580	4	95	theme	atomic	843:848	arg1	microscopy					856:865	atomic force microscopy	843:865	atomic force microscopy (AFM) force measurements	843:890	On the basis of atomic force microscopy (AFM) force measurements, an optimal sticky hydrogel scaffold was obtained through orthogonal experimental design.
28640580	4	95	theme	atomic	843:848	arg1	AFM					868:870	AFM	868:870	AFM	868:870	On the basis of atomic force microscopy (AFM) force measurements, an optimal sticky hydrogel scaffold was obtained through orthogonal experimental design.
28640580	3	96	with	arabic	779:784	arg1	cross-linking					812:824	calcium ion-mediated cross-linking	791:824	calcium ion-mediated cross-linking	791:824	Both chemical compositions and micro-/nanomorphological properties inherent from the natural Sundew hydrogel were mimicked using sodium alginate and gum arabic with calcium ion-mediated cross-linking.
28640580	7	97	theme	Sundew-inspired	1390:1404	arg1	hydrogel					1406:1413	the Sundew-inspired hydrogel	1386:1413	the Sundew-inspired hydrogel	1386:1413	In vitro drug release experiments confirmed that the Sundew-inspired hydrogel had a biphasic-kinetics release, which can facilitate both fast delivery of MG53 for improving the reepithelization process of the wounds and sustained release of the protein for treating chronic wounds.
28640580	3	98	theme	sodium	755:760	arg1	alginate					762:769	sodium alginate	755:769	sodium alginate	755:769	Both chemical compositions and micro-/nanomorphological properties inherent from the natural Sundew hydrogel were mimicked using sodium alginate and gum arabic with calcium ion-mediated cross-linking.
28640580	4	99	theme	microscopy	856:865	arg1	measurements					879:890	atomic force microscopy (AFM) force measurements	843:890	atomic force microscopy (AFM) force measurements	843:890	On the basis of atomic force microscopy (AFM) force measurements, an optimal sticky hydrogel scaffold was obtained through orthogonal experimental design.
28487199	5	0	theme	variables	717:725	arg1	optimization					695:706	Further optimization	687:706	Further optimization of these variables	687:725	Further optimization of these variables was done by four-factor central composite design.
28487199	6	1	theme	enzyme	947:952	arg1	levels					965:970	enzyme production levels	947:970	enzyme production levels	947:970	The model came out to be significant and statistical analysis of results showed that an appropriate ratio of chitin and colloidal chitin had resulted into enhancement in enzyme production levels.
28487199	1	2	theme	Humicola	176:183	arg1	ITCC					192:195	A novel thermophilic chitinase producing strain Humicola grisea ITCC 10,360.16	128:205	A novel thermophilic chitinase producing strain Humicola grisea ITCC 10,360.16	128:205	A novel thermophilic chitinase producing strain Humicola grisea ITCC 10,360.16 was isolated from soil of semi-arid desert region of Rajasthan.
28487199	4	3	theme	chitinase	633:641	arg1	production					643:652	chitinase production	633:652	chitinase production	633:652	Among the eight nutritional elements studied, chitin, colloidal chitin, KCl and yeast-extract were identified as the most critical variables for chitinase production by Plackett-Burman design first.
28487199	3	4	theme	Nutritional	352:362	arg1	requirement					364:374	Nutritional requirement	352:374	Nutritional requirement for maximum production of chitinase under submerged condition	352:436	Nutritional requirement for maximum production of chitinase under submerged condition was optimized using response surface methodology.
28487199	6	5	theme	chitin	886:891	arg1	ratio					877:881	an appropriate ratio	862:881	an appropriate ratio of chitin and colloidal chitin had	862:916	The model came out to be significant and statistical analysis of results showed that an appropriate ratio of chitin and colloidal chitin had resulted into enhancement in enzyme production levels.
28487199	1	6	theme	grisea	185:190	arg1	ITCC					192:195	A novel thermophilic chitinase producing strain Humicola grisea ITCC 10,360.16	128:205	A novel thermophilic chitinase producing strain Humicola grisea ITCC 10,360.16	128:205	A novel thermophilic chitinase producing strain Humicola grisea ITCC 10,360.16 was isolated from soil of semi-arid desert region of Rajasthan.
28487199	10	7	theme	large	1522:1526	arg1	production					1534:1543	large scale production	1522:1543	large scale production of bioactive chitooligosaccharides	1522:1578	Chitinase production from H. grisea and optimization of economic production medium heighten the employment of enzyme for large scale production of bioactive chitooligosaccharides.
28487199	0	8	theme	bioactive	95:103	arg1	chitooligosaccharides					105:125	bioactive chitooligosaccharides	95:125	bioactive chitooligosaccharides	95:125	Production of chitinase from thermophilic Humicola grisea and its application in production of bioactive chitooligosaccharides.
28487199	0	9	from	Production	0:9	arg1	production					81:90	production	81:90	production of bioactive chitooligosaccharides	81:125	Production of chitinase from thermophilic Humicola grisea and its application in production of bioactive chitooligosaccharides.
28487199	10	10	theme	enzyme	1511:1516	arg1	employment					1497:1506	the employment	1493:1506	the employment of enzyme	1493:1516	Chitinase production from H. grisea and optimization of economic production medium heighten the employment of enzyme for large scale production of bioactive chitooligosaccharides.
28487199	10	11	theme	economic	1457:1464	arg1	medium					1477:1482	economic production medium	1457:1482	economic production medium	1457:1482	Chitinase production from H. grisea and optimization of economic production medium heighten the employment of enzyme for large scale production of bioactive chitooligosaccharides.
28487199	3	12	theme	chitinase	402:410	arg1	production					388:397	maximum production	380:397	maximum production of chitinase under submerged condition	380:436	Nutritional requirement for maximum production of chitinase under submerged condition was optimized using response surface methodology.
28487199	9	13	theme	colloidal	1350:1358	arg1	chitin					1360:1365	colloidal chitin	1350:1365	colloidal chitin	1350:1365	Thin layer chromatography revealed that enzyme can effectively hydrolyze colloidal chitin to produce chitooligosaccharides.
28487199	10	14	theme	Chitinase	1401:1409	arg1	production					1411:1420	Chitinase production	1401:1420	Chitinase production from H. grisea	1401:1435	Chitinase production from H. grisea and optimization of economic production medium heighten the employment of enzyme for large scale production of bioactive chitooligosaccharides.
28487199	3	15	theme	maximum	380:386	arg1	production					388:397	maximum production	380:397	maximum production of chitinase under submerged condition	380:436	Nutritional requirement for maximum production of chitinase under submerged condition was optimized using response surface methodology.
28487199	0	16	from	grisea	51:56	arg1	Production					0:9	Production	0:9	Production of chitinase from thermophilic Humicola grisea	0:56	Production of chitinase from thermophilic Humicola grisea and its application in production of bioactive chitooligosaccharides.
28487199	0	16	from	grisea	51:56	arg1	application					66:76	its application	62:76	its application in production of bioactive chitooligosaccharides	62:125	Production of chitinase from thermophilic Humicola grisea and its application in production of bioactive chitooligosaccharides.
28487199	5	17	theme	Further	687:693	arg1	optimization					695:706	Further optimization	687:706	Further optimization of these variables	687:725	Further optimization of these variables was done by four-factor central composite design.
28487199	6	18	theme	appropriate	865:875	arg1	ratio					877:881	an appropriate ratio	862:881	an appropriate ratio of chitin and colloidal chitin had	862:916	The model came out to be significant and statistical analysis of results showed that an appropriate ratio of chitin and colloidal chitin had resulted into enhancement in enzyme production levels.
28487199	0	19	theme	chitooligosaccharides	105:125	arg1	production					81:90	production	81:90	production of bioactive chitooligosaccharides	81:125	Production of chitinase from thermophilic Humicola grisea and its application in production of bioactive chitooligosaccharides.
28487199	4	20	theme	colloidal	542:550	arg1	yeast-extract					568:580	yeast-extract	568:580	yeast-extract	568:580	Among the eight nutritional elements studied, chitin, colloidal chitin, KCl and yeast-extract were identified as the most critical variables for chitinase production by Plackett-Burman design first.
28487199	4	20	theme	colloidal	542:550	arg1	KCl					560:562	KCl	560:562	KCl	560:562	Among the eight nutritional elements studied, chitin, colloidal chitin, KCl and yeast-extract were identified as the most critical variables for chitinase production by Plackett-Burman design first.
28487199	4	20	theme	colloidal	542:550	arg1	chitin					534:539	chitin	534:539	chitin	534:539	Among the eight nutritional elements studied, chitin, colloidal chitin, KCl and yeast-extract were identified as the most critical variables for chitinase production by Plackett-Burman design first.
28487199	4	20	theme	colloidal	542:550	arg1	chitin					552:557	colloidal chitin	542:557	colloidal chitin	542:557	Among the eight nutritional elements studied, chitin, colloidal chitin, KCl and yeast-extract were identified as the most critical variables for chitinase production by Plackett-Burman design first.
28487199	4	20	theme	colloidal	542:550	arg1	variables					619:627	the most critical variables	601:627	the most critical variables for chitinase production	601:652	Among the eight nutritional elements studied, chitin, colloidal chitin, KCl and yeast-extract were identified as the most critical variables for chitinase production by Plackett-Burman design first.
28487199	3	21	theme	response	458:465	arg1	methodology					475:485	response surface methodology	458:485	response surface methodology	458:485	Nutritional requirement for maximum production of chitinase under submerged condition was optimized using response surface methodology.
28487199	7	22	theme	enhanced	1016:1023	arg1	production					1035:1044	enhanced chitinase production	1016:1044	enhanced chitinase production	1016:1044	Optimum concentration of the variables for enhanced chitinase production were 7.49, 4.91, 0.19 and 5.50 (gl-1) for chitin, colloidal chitin, KCl and yeast extract, respectively.
28487199	4	23	theme	Plackett-Burman	657:671	arg1	design					673:678	Plackett-Burman design	657:678	Plackett-Burman design	657:678	Among the eight nutritional elements studied, chitin, colloidal chitin, KCl and yeast-extract were identified as the most critical variables for chitinase production by Plackett-Burman design first.
28487199	8	24	theme	optimum	1261:1267	arg1	levels					1269:1274	their optimum levels	1255:1274	their optimum levels	1255:1274	1.43 fold enhancement in chitinase titres was attained in shake flasks, when the variables were used at their optimum levels.
28487199	4	25	theme	critical	610:617	arg1	yeast-extract					568:580	yeast-extract	568:580	yeast-extract	568:580	Among the eight nutritional elements studied, chitin, colloidal chitin, KCl and yeast-extract were identified as the most critical variables for chitinase production by Plackett-Burman design first.
28487199	4	25	theme	critical	610:617	arg1	KCl					560:562	KCl	560:562	KCl	560:562	Among the eight nutritional elements studied, chitin, colloidal chitin, KCl and yeast-extract were identified as the most critical variables for chitinase production by Plackett-Burman design first.
28487199	4	25	theme	critical	610:617	arg1	chitin					534:539	chitin	534:539	chitin	534:539	Among the eight nutritional elements studied, chitin, colloidal chitin, KCl and yeast-extract were identified as the most critical variables for chitinase production by Plackett-Burman design first.
28487199	4	25	theme	critical	610:617	arg1	chitin					552:557	colloidal chitin	542:557	colloidal chitin	542:557	Among the eight nutritional elements studied, chitin, colloidal chitin, KCl and yeast-extract were identified as the most critical variables for chitinase production by Plackett-Burman design first.
28487199	4	25	theme	critical	610:617	arg1	variables					619:627	the most critical variables	601:627	the most critical variables for chitinase production	601:652	Among the eight nutritional elements studied, chitin, colloidal chitin, KCl and yeast-extract were identified as the most critical variables for chitinase production by Plackett-Burman design first.
28487199	7	26	theme	yeast	1122:1126	arg1	extract					1128:1134	yeast extract	1122:1134	yeast extract	1122:1134	Optimum concentration of the variables for enhanced chitinase production were 7.49, 4.91, 0.19 and 5.50 (gl-1) for chitin, colloidal chitin, KCl and yeast extract, respectively.
28487199	7	27	theme	chitinase	1025:1033	arg1	production					1035:1044	enhanced chitinase production	1016:1044	enhanced chitinase production	1016:1044	Optimum concentration of the variables for enhanced chitinase production were 7.49, 4.91, 0.19 and 5.50 (gl-1) for chitin, colloidal chitin, KCl and yeast extract, respectively.
28487199	7	28	theme	colloidal	1096:1104	arg1	chitin					1106:1111	colloidal chitin	1096:1111	colloidal chitin	1096:1111	Optimum concentration of the variables for enhanced chitinase production were 7.49, 4.91, 0.19 and 5.50 (gl-1) for chitin, colloidal chitin, KCl and yeast extract, respectively.
28487199	10	29	theme	scale	1528:1532	arg1	production					1534:1543	large scale production	1522:1543	large scale production of bioactive chitooligosaccharides	1522:1578	Chitinase production from H. grisea and optimization of economic production medium heighten the employment of enzyme for large scale production of bioactive chitooligosaccharides.
28487199	7	30	theme	variables	1002:1010	arg1	concentration					981:993	Optimum concentration	973:993	Optimum concentration of the variables for enhanced chitinase production	973:1044	Optimum concentration of the variables for enhanced chitinase production were 7.49, 4.91, 0.19 and 5.50 (gl-1) for chitin, colloidal chitin, KCl and yeast extract, respectively.
28487199	2	31	theme	submerged	328:336	arg1	fermentation					338:349	submerged fermentation	328:349	submerged fermentation	328:349	Maximum enzyme production (116±3.45Ul-1) was achieved in submerged fermentation.
28487199	8	32	from	enhancement	1161:1171	arg1	titres					1186:1191	chitinase titres	1176:1191	chitinase titres	1176:1191	1.43 fold enhancement in chitinase titres was attained in shake flasks, when the variables were used at their optimum levels.
28487199	8	33	theme	shake	1209:1213	arg1	flasks					1215:1220	shake flasks	1209:1220	shake flasks	1209:1220	1.43 fold enhancement in chitinase titres was attained in shake flasks, when the variables were used at their optimum levels.
28487199	6	34	theme	results	842:848	arg1	analysis					830:837	statistical analysis	818:837	statistical analysis of results	818:848	The model came out to be significant and statistical analysis of results showed that an appropriate ratio of chitin and colloidal chitin had resulted into enhancement in enzyme production levels.
28487199	10	35	theme	production	1466:1475	arg1	medium					1477:1482	economic production medium	1457:1482	economic production medium	1457:1482	Chitinase production from H. grisea and optimization of economic production medium heighten the employment of enzyme for large scale production of bioactive chitooligosaccharides.
28487199	0	36	theme	chitinase	14:22	arg1	Production					0:9	Production	0:9	Production of chitinase from thermophilic Humicola grisea	0:56	Production of chitinase from thermophilic Humicola grisea and its application in production of bioactive chitooligosaccharides.
28487199	0	36	theme	chitinase	14:22	arg1	application					66:76	its application	62:76	its application in production of bioactive chitooligosaccharides	62:125	Production of chitinase from thermophilic Humicola grisea and its application in production of bioactive chitooligosaccharides.
28487199	4	37	theme	nutritional	504:514	arg1	elements					516:523	the eight nutritional elements	494:523	the eight nutritional elements studied	494:531	Among the eight nutritional elements studied, chitin, colloidal chitin, KCl and yeast-extract were identified as the most critical variables for chitinase production by Plackett-Burman design first.
28487199	0	38	theme	Humicola	42:49	arg1	grisea					51:56	thermophilic Humicola grisea	29:56	thermophilic Humicola grisea	29:56	Production of chitinase from thermophilic Humicola grisea and its application in production of bioactive chitooligosaccharides.
28487199	1	39	theme	novel	130:134	arg1	ITCC					192:195	A novel thermophilic chitinase producing strain Humicola grisea ITCC 10,360.16	128:205	A novel thermophilic chitinase producing strain Humicola grisea ITCC 10,360.16	128:205	A novel thermophilic chitinase producing strain Humicola grisea ITCC 10,360.16 was isolated from soil of semi-arid desert region of Rajasthan.
28487199	0	40	theme	thermophilic	29:40	arg1	grisea					51:56	thermophilic Humicola grisea	29:56	thermophilic Humicola grisea	29:56	Production of chitinase from thermophilic Humicola grisea and its application in production of bioactive chitooligosaccharides.
28487199	3	41	theme	surface	467:473	arg1	methodology					475:485	response surface methodology	458:485	response surface methodology	458:485	Nutritional requirement for maximum production of chitinase under submerged condition was optimized using response surface methodology.
28487199	1	42	attach	isolated	211:218	arg2	ITCC					192:195	A novel thermophilic chitinase producing strain Humicola grisea ITCC 10,360.16	128:205	A novel thermophilic chitinase producing strain Humicola grisea ITCC 10,360.16	128:205	A novel thermophilic chitinase producing strain Humicola grisea ITCC 10,360.16 was isolated from soil of semi-arid desert region of Rajasthan.
28487199	1	42	attach	isolated	211:218	arg1	soil					225:228	soil	225:228	soil of semi-arid desert region of Rajasthan	225:268	A novel thermophilic chitinase producing strain Humicola grisea ITCC 10,360.16 was isolated from soil of semi-arid desert region of Rajasthan.
28487199	1	43	theme	semi-arid	233:241	arg1	region					250:255	semi-arid desert region	233:255	semi-arid desert region of Rajasthan	233:268	A novel thermophilic chitinase producing strain Humicola grisea ITCC 10,360.16 was isolated from soil of semi-arid desert region of Rajasthan.
28487199	5	44	theme	four-factor	739:749	arg1	design					769:774	four-factor central composite design	739:774	four-factor central composite design	739:774	Further optimization of these variables was done by four-factor central composite design.
28487199	9	45	theme	Thin	1277:1280	arg1	chromatography					1288:1301	Thin layer chromatography	1277:1301	Thin layer chromatography	1277:1301	Thin layer chromatography revealed that enzyme can effectively hydrolyze colloidal chitin to produce chitooligosaccharides.
28487199	6	46	theme	production	954:963	arg1	levels					965:970	enzyme production levels	947:970	enzyme production levels	947:970	The model came out to be significant and statistical analysis of results showed that an appropriate ratio of chitin and colloidal chitin had resulted into enhancement in enzyme production levels.
28487199	10	47	theme	chitooligosaccharides	1558:1578	arg1	production					1534:1543	large scale production	1522:1543	large scale production of bioactive chitooligosaccharides	1522:1578	Chitinase production from H. grisea and optimization of economic production medium heighten the employment of enzyme for large scale production of bioactive chitooligosaccharides.
28487199	5	48	theme	central	751:757	arg1	design					769:774	four-factor central composite design	739:774	four-factor central composite design	739:774	Further optimization of these variables was done by four-factor central composite design.
28487199	9	49	theme	layer	1282:1286	arg1	chromatography					1288:1301	Thin layer chromatography	1277:1301	Thin layer chromatography	1277:1301	Thin layer chromatography revealed that enzyme can effectively hydrolyze colloidal chitin to produce chitooligosaccharides.
28487199	6	50	theme	statistical	818:828	arg1	analysis					830:837	statistical analysis	818:837	statistical analysis of results	818:848	The model came out to be significant and statistical analysis of results showed that an appropriate ratio of chitin and colloidal chitin had resulted into enhancement in enzyme production levels.
28487199	0	51	from	application	66:76	arg1	production					81:90	production	81:90	production of bioactive chitooligosaccharides	81:125	Production of chitinase from thermophilic Humicola grisea and its application in production of bioactive chitooligosaccharides.
28487199	10	52	theme	medium	1477:1482	arg1	optimization					1441:1452	optimization	1441:1452	optimization of economic production medium	1441:1482	Chitinase production from H. grisea and optimization of economic production medium heighten the employment of enzyme for large scale production of bioactive chitooligosaccharides.
28487199	10	52	theme	medium	1477:1482	arg1	production					1411:1420	Chitinase production	1401:1420	Chitinase production from H. grisea	1401:1435	Chitinase production from H. grisea and optimization of economic production medium heighten the employment of enzyme for large scale production of bioactive chitooligosaccharides.
28487199	5	53	theme	composite	759:767	arg1	design					769:774	four-factor central composite design	739:774	four-factor central composite design	739:774	Further optimization of these variables was done by four-factor central composite design.
28487199	6	54	theme	chitin	907:912	arg1	ratio					877:881	an appropriate ratio	862:881	an appropriate ratio of chitin and colloidal chitin had	862:916	The model came out to be significant and statistical analysis of results showed that an appropriate ratio of chitin and colloidal chitin had resulted into enhancement in enzyme production levels.
28487199	8	55	theme	fold	1156:1159	arg1	enhancement					1161:1171	1.43 fold enhancement	1151:1171	1.43 fold enhancement in chitinase titres	1151:1191	1.43 fold enhancement in chitinase titres was attained in shake flasks, when the variables were used at their optimum levels.
28487199	1	56	theme	thermophilic	136:147	arg1	ITCC					192:195	A novel thermophilic chitinase producing strain Humicola grisea ITCC 10,360.16	128:205	A novel thermophilic chitinase producing strain Humicola grisea ITCC 10,360.16	128:205	A novel thermophilic chitinase producing strain Humicola grisea ITCC 10,360.16 was isolated from soil of semi-arid desert region of Rajasthan.
28487199	1	57	theme	desert	243:248	arg1	region					250:255	semi-arid desert region	233:255	semi-arid desert region of Rajasthan	233:268	A novel thermophilic chitinase producing strain Humicola grisea ITCC 10,360.16 was isolated from soil of semi-arid desert region of Rajasthan.
28487199	8	58	used	used	1247:1250	arg2	variables					1232:1240	the variables	1228:1240	the variables	1228:1240	1.43 fold enhancement in chitinase titres was attained in shake flasks, when the variables were used at their optimum levels.
28487199	6	59	theme	colloidal	897:905	arg1	chitin					907:912	colloidal chitin	897:912	colloidal chitin	897:912	The model came out to be significant and statistical analysis of results showed that an appropriate ratio of chitin and colloidal chitin had resulted into enhancement in enzyme production levels.
28487199	2	60	theme	enzyme	279:284	arg1	116±3.45Ul-1					298:309	116±3.45Ul-1	298:309	116±3.45Ul-1	298:309	Maximum enzyme production (116±3.45Ul-1) was achieved in submerged fermentation.
28487199	2	60	theme	enzyme	279:284	arg1	production					286:295	Maximum enzyme production	271:295	Maximum enzyme production (116±3.45Ul-1)	271:310	Maximum enzyme production (116±3.45Ul-1) was achieved in submerged fermentation.
28487199	10	61	theme	bioactive	1548:1556	arg1	chitooligosaccharides					1558:1578	bioactive chitooligosaccharides	1548:1578	bioactive chitooligosaccharides	1548:1578	Chitinase production from H. grisea and optimization of economic production medium heighten the employment of enzyme for large scale production of bioactive chitooligosaccharides.
28487199	1	62	theme	chitinase	149:157	arg1	ITCC					192:195	A novel thermophilic chitinase producing strain Humicola grisea ITCC 10,360.16	128:205	A novel thermophilic chitinase producing strain Humicola grisea ITCC 10,360.16	128:205	A novel thermophilic chitinase producing strain Humicola grisea ITCC 10,360.16 was isolated from soil of semi-arid desert region of Rajasthan.
28487199	7	63	theme	Optimum	973:979	arg1	concentration					981:993	Optimum concentration	973:993	Optimum concentration of the variables for enhanced chitinase production	973:1044	Optimum concentration of the variables for enhanced chitinase production were 7.49, 4.91, 0.19 and 5.50 (gl-1) for chitin, colloidal chitin, KCl and yeast extract, respectively.
28487199	1	64	theme	region	250:255	arg1	soil					225:228	soil	225:228	soil of semi-arid desert region of Rajasthan	225:268	A novel thermophilic chitinase producing strain Humicola grisea ITCC 10,360.16 was isolated from soil of semi-arid desert region of Rajasthan.
28487199	8	65	theme	chitinase	1176:1184	arg1	titres					1186:1191	chitinase titres	1176:1191	chitinase titres	1176:1191	1.43 fold enhancement in chitinase titres was attained in shake flasks, when the variables were used at their optimum levels.
28487199	10	66	from	grisea	1430:1435	arg1	optimization					1441:1452	optimization	1441:1452	optimization of economic production medium	1441:1482	Chitinase production from H. grisea and optimization of economic production medium heighten the employment of enzyme for large scale production of bioactive chitooligosaccharides.
28487199	10	66	from	grisea	1430:1435	arg1	production					1411:1420	Chitinase production	1401:1420	Chitinase production from H. grisea	1401:1435	Chitinase production from H. grisea and optimization of economic production medium heighten the employment of enzyme for large scale production of bioactive chitooligosaccharides.
28487199	2	67	theme	Maximum	271:277	arg1	116±3.45Ul-1					298:309	116±3.45Ul-1	298:309	116±3.45Ul-1	298:309	Maximum enzyme production (116±3.45Ul-1) was achieved in submerged fermentation.
28487199	2	67	theme	Maximum	271:277	arg1	production					286:295	Maximum enzyme production	271:295	Maximum enzyme production (116±3.45Ul-1)	271:310	Maximum enzyme production (116±3.45Ul-1) was achieved in submerged fermentation.
28487199	1	68	theme	producing	159:167	arg1	ITCC					192:195	A novel thermophilic chitinase producing strain Humicola grisea ITCC 10,360.16	128:205	A novel thermophilic chitinase producing strain Humicola grisea ITCC 10,360.16	128:205	A novel thermophilic chitinase producing strain Humicola grisea ITCC 10,360.16 was isolated from soil of semi-arid desert region of Rajasthan.
28487199	3	69	theme	submerged	418:426	arg1	condition					428:436	submerged condition	418:436	submerged condition	418:436	Nutritional requirement for maximum production of chitinase under submerged condition was optimized using response surface methodology.
28487199	10	70	theme	H.	1427:1428	arg1	grisea					1430:1435	H. grisea	1427:1435	H. grisea	1427:1435	Chitinase production from H. grisea and optimization of economic production medium heighten the employment of enzyme for large scale production of bioactive chitooligosaccharides.
28487199	1	71	theme	strain	169:174	arg1	ITCC					192:195	A novel thermophilic chitinase producing strain Humicola grisea ITCC 10,360.16	128:205	A novel thermophilic chitinase producing strain Humicola grisea ITCC 10,360.16	128:205	A novel thermophilic chitinase producing strain Humicola grisea ITCC 10,360.16 was isolated from soil of semi-arid desert region of Rajasthan.
28487199	1	72	theme	Rajasthan	260:268	arg1	region					250:255	semi-arid desert region	233:255	semi-arid desert region of Rajasthan	233:268	A novel thermophilic chitinase producing strain Humicola grisea ITCC 10,360.16 was isolated from soil of semi-arid desert region of Rajasthan.
26876865	0	0	theme	butylene	68:75	arg1	poly					63:66	melt-spun poly	53:66	melt-spun poly(butylene succinate)	53:86	Effect of stretching on the mechanical properties in melt-spun poly(butylene succinate)/microfibrillated cellulose (MFC) nanocomposites.
26876865	0	0	theme	butylene	68:75	arg1	succinate					77:85	butylene succinate	68:85	butylene succinate	68:85	Effect of stretching on the mechanical properties in melt-spun poly(butylene succinate)/microfibrillated cellulose (MFC) nanocomposites.
26876865	4	1	theme	melt	884:887	arg1	spinning					889:896	the melt spinning	880:896	the melt spinning	880:896	The dispersion, alignment of the MFC and interfacial crystalline structure in the composite fibers are significantly influenced by the stretching force during the melt spinning.
26876865	4	2	theme	MFC	754:756	arg1	alignment					737:745	alignment	737:745	alignment of the MFC	737:756	The dispersion, alignment of the MFC and interfacial crystalline structure in the composite fibers are significantly influenced by the stretching force during the melt spinning.
26876865	4	2	theme	MFC	754:756	arg1	dispersion					725:734	The dispersion	721:734	The dispersion	721:734	The dispersion, alignment of the MFC and interfacial crystalline structure in the composite fibers are significantly influenced by the stretching force during the melt spinning.
26876865	4	2	theme	MFC	754:756	arg1	structure					786:794	interfacial crystalline structure	762:794	interfacial crystalline structure	762:794	The dispersion, alignment of the MFC and interfacial crystalline structure in the composite fibers are significantly influenced by the stretching force during the melt spinning.
26876865	3	3	theme	mechanical	600:609	arg1	properties					611:620	mechanical properties	600:620	mechanical properties	600:620	And the effect of MFC on the crystalline structure and mechanical properties were systematically investigated for the melt-spun fibers prepared with two different draw ratios.
26876865	5	4	from	SEM	1046:1048	arg1	fibers					1076:1081	the composite fibers	1062:1081	the composite fibers prepared at the high draw ratio	1062:1113	The possible formation of nanohybrid shish kebab (NHSK) superstructure where aligned MFC as shish and PBS lamellae as kebab has been suggested via SEM and SAXS in the composite fibers prepared at the high draw ratio.
26876865	0	5	from	Effect	0:5	arg1	properties					39:48	the mechanical properties	24:48	the mechanical properties in melt-spun poly(butylene succinate)	24:86	Effect of stretching on the mechanical properties in melt-spun poly(butylene succinate)/microfibrillated cellulose (MFC) nanocomposites.
26876865	5	6	theme	PBS	1001:1003	arg1	lamellae					1005:1012	PBS lamellae	1001:1012	PBS lamellae	1001:1012	The possible formation of nanohybrid shish kebab (NHSK) superstructure where aligned MFC as shish and PBS lamellae as kebab has been suggested via SEM and SAXS in the composite fibers prepared at the high draw ratio.
26876865	6	7	theme	tensile	1137:1143	arg1	strength					1145:1152	tensile strength	1137:1152	tensile strength	1137:1152	Large improvement in tensile strength has been realized at the high draw ratio due to the enhanced orientation and dispersion of MFC as well as the formation of NHSK.
26876865	3	8	theme	different	698:706	arg1	ratios					713:718	two different draw ratios	694:718	two different draw ratios	694:718	And the effect of MFC on the crystalline structure and mechanical properties were systematically investigated for the melt-spun fibers prepared with two different draw ratios.
26876865	4	9	theme	stretching	856:865	arg1	force					867:871	the stretching force	852:871	the stretching force during the melt spinning	852:896	The dispersion, alignment of the MFC and interfacial crystalline structure in the composite fibers are significantly influenced by the stretching force during the melt spinning.
26876865	6	10	theme	enhanced	1206:1213	arg1	orientation					1215:1225	orientation	1215:1225	orientation	1215:1225	Large improvement in tensile strength has been realized at the high draw ratio due to the enhanced orientation and dispersion of MFC as well as the formation of NHSK.
26876865	5	11	theme	NHSK	949:952	arg1	superstructure					955:968	nanohybrid shish kebab (NHSK) superstructure	925:968	nanohybrid shish kebab (NHSK) superstructure where aligned MFC as shish and PBS lamellae as kebab	925:1021	The possible formation of nanohybrid shish kebab (NHSK) superstructure where aligned MFC as shish and PBS lamellae as kebab has been suggested via SEM and SAXS in the composite fibers prepared at the high draw ratio.
26876865	6	12	from	improvement	1122:1132	arg1	strength					1145:1152	tensile strength	1137:1152	tensile strength	1137:1152	Large improvement in tensile strength has been realized at the high draw ratio due to the enhanced orientation and dispersion of MFC as well as the formation of NHSK.
26876865	0	13	theme	cellulose	105:113	arg1	nanocomposites					121:134	cellulose (MFC) nanocomposites	105:134	cellulose (MFC) nanocomposites	105:134	Effect of stretching on the mechanical properties in melt-spun poly(butylene succinate)/microfibrillated cellulose (MFC) nanocomposites.
26876865	0	14	from	properties	39:48	arg1	poly					63:66	melt-spun poly	53:66	melt-spun poly(butylene succinate)	53:86	Effect of stretching on the mechanical properties in melt-spun poly(butylene succinate)/microfibrillated cellulose (MFC) nanocomposites.
26876865	0	14	from	properties	39:48	arg1	succinate					77:85	butylene succinate	68:85	butylene succinate	68:85	Effect of stretching on the mechanical properties in melt-spun poly(butylene succinate)/microfibrillated cellulose (MFC) nanocomposites.
26876865	6	15	theme	NHSK	1277:1280	arg1	orientation					1215:1225	orientation	1215:1225	orientation	1215:1225	Large improvement in tensile strength has been realized at the high draw ratio due to the enhanced orientation and dispersion of MFC as well as the formation of NHSK.
26876865	6	15	theme	NHSK	1277:1280	arg1	formation					1264:1272	the formation	1260:1272	the enhanced orientation and dispersion of MFC as well as the formation of NHSK	1202:1280	Large improvement in tensile strength has been realized at the high draw ratio due to the enhanced orientation and dispersion of MFC as well as the formation of NHSK.
26876865	6	15	theme	NHSK	1277:1280	arg1	dispersion					1231:1240	dispersion	1231:1240	dispersion	1231:1240	Large improvement in tensile strength has been realized at the high draw ratio due to the enhanced orientation and dispersion of MFC as well as the formation of NHSK.
26876865	4	16	theme	crystalline	774:784	arg1	dispersion					725:734	The dispersion	721:734	The dispersion	721:734	The dispersion, alignment of the MFC and interfacial crystalline structure in the composite fibers are significantly influenced by the stretching force during the melt spinning.
26876865	4	16	theme	crystalline	774:784	arg1	structure					786:794	interfacial crystalline structure	762:794	interfacial crystalline structure	762:794	The dispersion, alignment of the MFC and interfacial crystalline structure in the composite fibers are significantly influenced by the stretching force during the melt spinning.
26876865	4	17	from	dispersion	725:734	arg1	fibers					813:818	the composite fibers	799:818	the composite fibers	799:818	The dispersion, alignment of the MFC and interfacial crystalline structure in the composite fibers are significantly influenced by the stretching force during the melt spinning.
26876865	5	18	theme	superstructure	955:968	arg1	formation					912:920	The possible formation	899:920	The possible formation of nanohybrid shish kebab (NHSK) superstructure where aligned MFC as shish and PBS lamellae as kebab	899:1021	The possible formation of nanohybrid shish kebab (NHSK) superstructure where aligned MFC as shish and PBS lamellae as kebab has been suggested via SEM and SAXS in the composite fibers prepared at the high draw ratio.
26876865	1	19	theme	poly	157:160	arg1	composites					209:218	poly(butylene succinate)/microfibrillated cellulose composites	157:218	poly(butylene succinate)/microfibrillated cellulose composites with high performance	157:240	In order to prepare poly(butylene succinate)/microfibrillated cellulose composites with high performance, in this work, microfibrillated cellulose (MFC) was first treated by acetylchloride with ball-milling to improve its interfacial compatibility with poly(butylene succinate) (PBS).
26876865	5	20	theme	composite	1066:1074	arg1	fibers					1076:1081	the composite fibers	1062:1081	the composite fibers prepared at the high draw ratio	1062:1113	The possible formation of nanohybrid shish kebab (NHSK) superstructure where aligned MFC as shish and PBS lamellae as kebab has been suggested via SEM and SAXS in the composite fibers prepared at the high draw ratio.
26876865	6	21	theme	due	1195:1197	arg1	ratio					1189:1193	the high draw ratio	1175:1193	the high draw ratio due to the enhanced orientation and dispersion of MFC as well as the formation of NHSK	1175:1280	Large improvement in tensile strength has been realized at the high draw ratio due to the enhanced orientation and dispersion of MFC as well as the formation of NHSK.
26876865	5	22	theme	nanohybrid	925:934	arg1	superstructure					955:968	nanohybrid shish kebab (NHSK) superstructure	925:968	nanohybrid shish kebab (NHSK) superstructure where aligned MFC as shish and PBS lamellae as kebab	925:1021	The possible formation of nanohybrid shish kebab (NHSK) superstructure where aligned MFC as shish and PBS lamellae as kebab has been suggested via SEM and SAXS in the composite fibers prepared at the high draw ratio.
26876865	1	23	theme	butylene	162:169	arg1	poly					157:160	poly	157:160	poly(butylene succinate)/microfibrillated cellulose composites with high performance	157:240	In order to prepare poly(butylene succinate)/microfibrillated cellulose composites with high performance, in this work, microfibrillated cellulose (MFC) was first treated by acetylchloride with ball-milling to improve its interfacial compatibility with poly(butylene succinate) (PBS).
26876865	1	23	theme	butylene	162:169	arg1	succinate					171:179	butylene succinate	162:179	butylene succinate	162:179	In order to prepare poly(butylene succinate)/microfibrillated cellulose composites with high performance, in this work, microfibrillated cellulose (MFC) was first treated by acetylchloride with ball-milling to improve its interfacial compatibility with poly(butylene succinate) (PBS).
26876865	5	24	from	SAXS	1054:1057	arg1	fibers					1076:1081	the composite fibers	1062:1081	the composite fibers prepared at the high draw ratio	1062:1113	The possible formation of nanohybrid shish kebab (NHSK) superstructure where aligned MFC as shish and PBS lamellae as kebab has been suggested via SEM and SAXS in the composite fibers prepared at the high draw ratio.
26876865	1	25	theme	microfibrillated	257:272	arg1	cellulose					274:282	microfibrillated cellulose	257:282	microfibrillated cellulose (MFC)	257:288	In order to prepare poly(butylene succinate)/microfibrillated cellulose composites with high performance, in this work, microfibrillated cellulose (MFC) was first treated by acetylchloride with ball-milling to improve its interfacial compatibility with poly(butylene succinate) (PBS).
26876865	1	25	theme	microfibrillated	257:272	arg1	MFC					285:287	MFC	285:287	MFC	285:287	In order to prepare poly(butylene succinate)/microfibrillated cellulose composites with high performance, in this work, microfibrillated cellulose (MFC) was first treated by acetylchloride with ball-milling to improve its interfacial compatibility with poly(butylene succinate) (PBS).
26876865	5	26	theme	shish	936:940	arg1	superstructure					955:968	nanohybrid shish kebab (NHSK) superstructure	925:968	nanohybrid shish kebab (NHSK) superstructure where aligned MFC as shish and PBS lamellae as kebab	925:1021	The possible formation of nanohybrid shish kebab (NHSK) superstructure where aligned MFC as shish and PBS lamellae as kebab has been suggested via SEM and SAXS in the composite fibers prepared at the high draw ratio.
26876865	0	27	theme	stretching	10:19	arg1	Effect					0:5	Effect	0:5	Effect of stretching on the mechanical properties in melt-spun poly(butylene succinate)	0:86	Effect of stretching on the mechanical properties in melt-spun poly(butylene succinate)/microfibrillated cellulose (MFC) nanocomposites.
26876865	2	28	theme	MFC	519:521	arg1	orientation					504:514	orientation	504:514	orientation	504:514	Then melt stretching processing was adopted to further improve the dispersion and orientation of MFC in as-spun PBS fiber.
26876865	2	28	theme	MFC	519:521	arg1	dispersion					489:498	dispersion	489:498	dispersion	489:498	Then melt stretching processing was adopted to further improve the dispersion and orientation of MFC in as-spun PBS fiber.
26876865	1	29	theme	interfacial	359:369	arg1	compatibility					371:383	its interfacial compatibility	355:383	its interfacial compatibility with poly(butylene succinate) (PBS)	355:419	In order to prepare poly(butylene succinate)/microfibrillated cellulose composites with high performance, in this work, microfibrillated cellulose (MFC) was first treated by acetylchloride with ball-milling to improve its interfacial compatibility with poly(butylene succinate) (PBS).
26876865	5	30	theme	kebab	942:946	arg1	superstructure					955:968	nanohybrid shish kebab (NHSK) superstructure	925:968	nanohybrid shish kebab (NHSK) superstructure where aligned MFC as shish and PBS lamellae as kebab	925:1021	The possible formation of nanohybrid shish kebab (NHSK) superstructure where aligned MFC as shish and PBS lamellae as kebab has been suggested via SEM and SAXS in the composite fibers prepared at the high draw ratio.
26876865	3	31	from	effect	553:558	arg1	structure					586:594	the crystalline structure	570:594	the crystalline structure	570:594	And the effect of MFC on the crystalline structure and mechanical properties were systematically investigated for the melt-spun fibers prepared with two different draw ratios.
26876865	3	31	from	effect	553:558	arg1	properties					611:620	mechanical properties	600:620	mechanical properties	600:620	And the effect of MFC on the crystalline structure and mechanical properties were systematically investigated for the melt-spun fibers prepared with two different draw ratios.
26876865	0	32	theme	mechanical	28:37	arg1	properties					39:48	the mechanical properties	24:48	the mechanical properties in melt-spun poly(butylene succinate)	24:86	Effect of stretching on the mechanical properties in melt-spun poly(butylene succinate)/microfibrillated cellulose (MFC) nanocomposites.
26876865	6	33	theme	draw	1184:1187	arg1	ratio					1189:1193	the high draw ratio	1175:1193	the high draw ratio due to the enhanced orientation and dispersion of MFC as well as the formation of NHSK	1175:1280	Large improvement in tensile strength has been realized at the high draw ratio due to the enhanced orientation and dispersion of MFC as well as the formation of NHSK.
26876865	1	34	with	compatibility	371:383	arg1	poly					390:393	poly	390:393	poly(butylene succinate) (PBS)	390:419	In order to prepare poly(butylene succinate)/microfibrillated cellulose composites with high performance, in this work, microfibrillated cellulose (MFC) was first treated by acetylchloride with ball-milling to improve its interfacial compatibility with poly(butylene succinate) (PBS).
26876865	1	34	with	compatibility	371:383	arg1	succinate					404:412	butylene succinate	395:412	butylene succinate	395:412	In order to prepare poly(butylene succinate)/microfibrillated cellulose composites with high performance, in this work, microfibrillated cellulose (MFC) was first treated by acetylchloride with ball-milling to improve its interfacial compatibility with poly(butylene succinate) (PBS).
26876865	1	34	with	compatibility	371:383	arg1	PBS					416:418	PBS	416:418	PBS	416:418	In order to prepare poly(butylene succinate)/microfibrillated cellulose composites with high performance, in this work, microfibrillated cellulose (MFC) was first treated by acetylchloride with ball-milling to improve its interfacial compatibility with poly(butylene succinate) (PBS).
26876865	1	35	theme	/microfibrillated	181:197	arg1	composites					209:218	poly(butylene succinate)/microfibrillated cellulose composites	157:218	poly(butylene succinate)/microfibrillated cellulose composites with high performance	157:240	In order to prepare poly(butylene succinate)/microfibrillated cellulose composites with high performance, in this work, microfibrillated cellulose (MFC) was first treated by acetylchloride with ball-milling to improve its interfacial compatibility with poly(butylene succinate) (PBS).
26876865	2	36	theme	stretching	432:441	arg1	processing					443:452	melt stretching processing	427:452	melt stretching processing	427:452	Then melt stretching processing was adopted to further improve the dispersion and orientation of MFC in as-spun PBS fiber.
26876865	5	37	theme	high	1099:1102	arg1	ratio					1109:1113	the high draw ratio	1095:1113	the high draw ratio	1095:1113	The possible formation of nanohybrid shish kebab (NHSK) superstructure where aligned MFC as shish and PBS lamellae as kebab has been suggested via SEM and SAXS in the composite fibers prepared at the high draw ratio.
26876865	6	38	theme	high	1179:1182	arg1	ratio					1189:1193	the high draw ratio	1175:1193	the high draw ratio due to the enhanced orientation and dispersion of MFC as well as the formation of NHSK	1175:1280	Large improvement in tensile strength has been realized at the high draw ratio due to the enhanced orientation and dispersion of MFC as well as the formation of NHSK.
26876865	1	39	theme	cellulose	199:207	arg1	composites					209:218	poly(butylene succinate)/microfibrillated cellulose composites	157:218	poly(butylene succinate)/microfibrillated cellulose composites with high performance	157:240	In order to prepare poly(butylene succinate)/microfibrillated cellulose composites with high performance, in this work, microfibrillated cellulose (MFC) was first treated by acetylchloride with ball-milling to improve its interfacial compatibility with poly(butylene succinate) (PBS).
26876865	2	40	theme	melt	427:430	arg1	processing					443:452	melt stretching processing	427:452	melt stretching processing	427:452	Then melt stretching processing was adopted to further improve the dispersion and orientation of MFC in as-spun PBS fiber.
26876865	5	41	theme	draw	1104:1107	arg1	ratio					1109:1113	the high draw ratio	1095:1113	the high draw ratio	1095:1113	The possible formation of nanohybrid shish kebab (NHSK) superstructure where aligned MFC as shish and PBS lamellae as kebab has been suggested via SEM and SAXS in the composite fibers prepared at the high draw ratio.
26876865	4	42	theme	composite	803:811	arg1	fibers					813:818	the composite fibers	799:818	the composite fibers	799:818	The dispersion, alignment of the MFC and interfacial crystalline structure in the composite fibers are significantly influenced by the stretching force during the melt spinning.
26876865	6	43	theme	MFC	1245:1247	arg1	orientation					1215:1225	orientation	1215:1225	orientation	1215:1225	Large improvement in tensile strength has been realized at the high draw ratio due to the enhanced orientation and dispersion of MFC as well as the formation of NHSK.
26876865	6	43	theme	MFC	1245:1247	arg1	formation					1264:1272	the formation	1260:1272	the enhanced orientation and dispersion of MFC as well as the formation of NHSK	1202:1280	Large improvement in tensile strength has been realized at the high draw ratio due to the enhanced orientation and dispersion of MFC as well as the formation of NHSK.
26876865	6	43	theme	MFC	1245:1247	arg1	dispersion					1231:1240	dispersion	1231:1240	dispersion	1231:1240	Large improvement in tensile strength has been realized at the high draw ratio due to the enhanced orientation and dispersion of MFC as well as the formation of NHSK.
26876865	3	44	theme	melt-spun	663:671	arg1	fibers					673:678	the melt-spun fibers	659:678	the melt-spun fibers prepared with two different draw ratios	659:718	And the effect of MFC on the crystalline structure and mechanical properties were systematically investigated for the melt-spun fibers prepared with two different draw ratios.
26876865	2	45	theme	PBS	534:536	arg1	fiber					538:542	as-spun PBS fiber	526:542	as-spun PBS fiber	526:542	Then melt stretching processing was adopted to further improve the dispersion and orientation of MFC in as-spun PBS fiber.
26876865	1	46	theme	butylene	395:402	arg1	poly					390:393	poly	390:393	poly(butylene succinate) (PBS)	390:419	In order to prepare poly(butylene succinate)/microfibrillated cellulose composites with high performance, in this work, microfibrillated cellulose (MFC) was first treated by acetylchloride with ball-milling to improve its interfacial compatibility with poly(butylene succinate) (PBS).
26876865	1	46	theme	butylene	395:402	arg1	succinate					404:412	butylene succinate	395:412	butylene succinate	395:412	In order to prepare poly(butylene succinate)/microfibrillated cellulose composites with high performance, in this work, microfibrillated cellulose (MFC) was first treated by acetylchloride with ball-milling to improve its interfacial compatibility with poly(butylene succinate) (PBS).
26876865	5	47	theme	possible	903:910	arg1	formation					912:920	The possible formation	899:920	The possible formation of nanohybrid shish kebab (NHSK) superstructure where aligned MFC as shish and PBS lamellae as kebab	899:1021	The possible formation of nanohybrid shish kebab (NHSK) superstructure where aligned MFC as shish and PBS lamellae as kebab has been suggested via SEM and SAXS in the composite fibers prepared at the high draw ratio.
26876865	2	48	theme	as-spun	526:532	arg1	fiber					538:542	as-spun PBS fiber	526:542	as-spun PBS fiber	526:542	Then melt stretching processing was adopted to further improve the dispersion and orientation of MFC in as-spun PBS fiber.
26876865	3	49	theme	crystalline	574:584	arg1	structure					586:594	the crystalline structure	570:594	the crystalline structure	570:594	And the effect of MFC on the crystalline structure and mechanical properties were systematically investigated for the melt-spun fibers prepared with two different draw ratios.
26876865	2	50	from	orientation	504:514	arg1	fiber					538:542	as-spun PBS fiber	526:542	as-spun PBS fiber	526:542	Then melt stretching processing was adopted to further improve the dispersion and orientation of MFC in as-spun PBS fiber.
26876865	2	51	from	dispersion	489:498	arg1	fiber					538:542	as-spun PBS fiber	526:542	as-spun PBS fiber	526:542	Then melt stretching processing was adopted to further improve the dispersion and orientation of MFC in as-spun PBS fiber.
26876865	3	52	theme	MFC	563:565	arg1	effect					553:558	the effect	549:558	the effect of MFC on the crystalline structure and mechanical properties	549:620	And the effect of MFC on the crystalline structure and mechanical properties were systematically investigated for the melt-spun fibers prepared with two different draw ratios.
26876865	6	53	theme	Large	1116:1120	arg1	improvement					1122:1132	Large improvement	1116:1132	Large improvement in tensile strength	1116:1152	Large improvement in tensile strength has been realized at the high draw ratio due to the enhanced orientation and dispersion of MFC as well as the formation of NHSK.
26876865	1	54	theme	high	225:228	arg1	performance					230:240	high performance	225:240	high performance	225:240	In order to prepare poly(butylene succinate)/microfibrillated cellulose composites with high performance, in this work, microfibrillated cellulose (MFC) was first treated by acetylchloride with ball-milling to improve its interfacial compatibility with poly(butylene succinate) (PBS).
26876865	0	55	theme	melt-spun	53:61	arg1	poly					63:66	melt-spun poly	53:66	melt-spun poly(butylene succinate)	53:86	Effect of stretching on the mechanical properties in melt-spun poly(butylene succinate)/microfibrillated cellulose (MFC) nanocomposites.
26876865	0	55	theme	melt-spun	53:61	arg1	succinate					77:85	butylene succinate	68:85	butylene succinate	68:85	Effect of stretching on the mechanical properties in melt-spun poly(butylene succinate)/microfibrillated cellulose (MFC) nanocomposites.
26876865	3	56	dep	ratios	713:718	arg1	draw					708:711	draw	708:711	draw	708:711	And the effect of MFC on the crystalline structure and mechanical properties were systematically investigated for the melt-spun fibers prepared with two different draw ratios.
26876865	4	57	theme	interfacial	762:772	arg1	dispersion					725:734	The dispersion	721:734	The dispersion	721:734	The dispersion, alignment of the MFC and interfacial crystalline structure in the composite fibers are significantly influenced by the stretching force during the melt spinning.
26876865	4	57	theme	interfacial	762:772	arg1	structure					786:794	interfacial crystalline structure	762:794	interfacial crystalline structure	762:794	The dispersion, alignment of the MFC and interfacial crystalline structure in the composite fibers are significantly influenced by the stretching force during the melt spinning.
26876865	1	58	with	composites	209:218	arg1	performance					230:240	high performance	225:240	high performance	225:240	In order to prepare poly(butylene succinate)/microfibrillated cellulose composites with high performance, in this work, microfibrillated cellulose (MFC) was first treated by acetylchloride with ball-milling to improve its interfacial compatibility with poly(butylene succinate) (PBS).
25934513	13	0	theme	MAPK-dependent	2864:2877	arg1	phosphorylation					2886:2900	MAPK-dependent linker phosphorylation	2864:2900	MAPK-dependent linker phosphorylation of Smad2/3	2864:2911	CONCLUSIONS CASE blocked MAPK activation, MAPK-dependent linker phosphorylation of Smad2/3, Smad4 expression, Imp7 expression and their nuclear import leading to significant down-regulation of PAI-1 gene expression; further highlighting the multi-target anti-HCC effect of CASE and its potential drug candidature.
25934513	9	1	theme	blot	1885:1888	arg1	technique					1890:1898	western blot technique	1877:1898	western blot technique	1877:1898	The expression of pERK, pJNK, pp38 and PAI-1 gene were monitored by using western blot technique.
25934513	3	2	theme	anti-hepatocarcinogenic	708:730	arg1	effects					732:738	their anti-hepatocarcinogenic effects	702:738	their anti-hepatocarcinogenic effects	702:738	In response, recent studies on these two herbs have focused on elucidating their mechanisms of action, particularly with regards to their anti-hepatocarcinogenic effects.
25934513	1	3	from	treatment	363:371	arg1	history					314:320	a long history	307:320	a long history of extensive ethnobotanical usage in the treatment of liver-related diseases over many centuries	307:417	ETHNOPHARMACOLOGICAL RELEVANCE Astragalus membranaceus Bunge (Leguminosae) and Salvia miltiorrhiza Bunge (Lamiaceae) are two important Chinese herbs with a long history of extensive ethnobotanical usage in the treatment of liver-related diseases over many centuries.
25934513	8	4	theme	pSmad2/3	1719:1726	arg1	phosphorylation					1671:1685	phosphorylation	1671:1685	phosphorylation	1671:1685	Also phosphorylation and subcellular distribution of pSmad2/3, Smad4 and Imp7/8 in TGF-β1-stimulated HSC and HepG2 cells were monitored.
25934513	8	4	theme	pSmad2/3	1719:1726	arg1	distribution					1703:1714	subcellular distribution	1691:1714	subcellular distribution	1691:1714	Also phosphorylation and subcellular distribution of pSmad2/3, Smad4 and Imp7/8 in TGF-β1-stimulated HSC and HepG2 cells were monitored.
25934513	12	5	theme	HepG2	2733:2737	arg1	cells					2739:2743	HepG2 cells	2733:2743	HepG2 cells	2733:2743	Also, CASE concentration dependently inhibited pSmad2C/L, pSmad3L, Smad4, Imp7/8 and their nuclear import; it had no effect on pSmad3C in HepG2 cells; significantly decreased PAI-1 gene expression in both in vitro and in vivo.
25934513	12	6	theme	PAI-1	2770:2774	arg1	expression					2781:2790	PAI-1 gene expression	2770:2790	PAI-1 gene expression	2770:2790	Also, CASE concentration dependently inhibited pSmad2C/L, pSmad3L, Smad4, Imp7/8 and their nuclear import; it had no effect on pSmad3C in HepG2 cells; significantly decreased PAI-1 gene expression in both in vitro and in vivo.
25934513	6	7	theme	TGF-β/Smad	1328:1337	arg1	signaling					1339:1347	TGF-β/Smad signaling	1328:1347	TGF-β/Smad signaling using both cell and animal models of HCC	1328:1388	To elucidate further, we studied the effect of CASE on the MAPK pathway and how it affects MAPK-dependent regulation of TGF-β/Smad signaling using both cell and animal models of HCC.
25934513	8	8	theme	Smad4	1729:1733	arg1	phosphorylation					1671:1685	phosphorylation	1671:1685	phosphorylation	1671:1685	Also phosphorylation and subcellular distribution of pSmad2/3, Smad4 and Imp7/8 in TGF-β1-stimulated HSC and HepG2 cells were monitored.
25934513	8	8	theme	Smad4	1729:1733	arg1	distribution					1703:1714	subcellular distribution	1691:1714	subcellular distribution	1691:1714	Also phosphorylation and subcellular distribution of pSmad2/3, Smad4 and Imp7/8 in TGF-β1-stimulated HSC and HepG2 cells were monitored.
25934513	1	9	theme	long	309:312	arg1	history					314:320	a long history	307:320	a long history of extensive ethnobotanical usage in the treatment of liver-related diseases over many centuries	307:417	ETHNOPHARMACOLOGICAL RELEVANCE Astragalus membranaceus Bunge (Leguminosae) and Salvia miltiorrhiza Bunge (Lamiaceae) are two important Chinese herbs with a long history of extensive ethnobotanical usage in the treatment of liver-related diseases over many centuries.
25934513	7	10	theme	MAPKs	1519:1523	arg1	activation					1501:1510	the activation	1497:1510	the activation of the MAPKs (pERK, pJNK and pp38) in TGF-β1-stimulated hepatic stellate cells (HSCs), HepG2 cells	1497:1609	MATERIALS AND METHODS We used immunofluorescence and western blot techniques to monitor effect of CASE on the activation of the MAPKs (pERK, pJNK and pp38) in TGF-β1-stimulated hepatic stellate cells (HSCs), HepG2 cells and also diethylnitrosamine (DEN)-induced HCC in rats.
25934513	11	11	theme	concentration-dependent	2378:2400	arg1	manner					2402:2407	a concentration-dependent manner	2376:2407	a concentration-dependent manner	2376:2407	And the expression of Imp7/8 and their subcellular distribution were assessed by both immunofluorescence and western blot techniques, while PAI-1 gene expression was assessed by western blot RESULTS In vitro, CASE in a concentration-dependent manner increased the expression of pp38 but decreased the expression of pERK and pJNK; however, in vivo, CASE in a dose dependent manner decreased the expression of pERK, pJNK as well as pp38.
25934513	10	12	theme	domain-specific	1923:1937	arg1	phosphorylation					1939:1953	domain-specific phosphorylation	1923:1953	domain-specific phosphorylation of Smad2/3	1923:1964	The effect of CASE on domain-specific phosphorylation of Smad2/3 and their subcellular distribution, and the expression of Smad4 and its subcellular distribution in TGF-β1-stimulated HSCs and HepG2 cells were evaluated by using immunofluorescence technique.
25934513	11	13	theme	subcellular	2198:2208	arg1	distribution					2210:2221	their subcellular distribution	2192:2221	their subcellular distribution	2192:2221	And the expression of Imp7/8 and their subcellular distribution were assessed by both immunofluorescence and western blot techniques, while PAI-1 gene expression was assessed by western blot RESULTS In vitro, CASE in a concentration-dependent manner increased the expression of pp38 but decreased the expression of pERK and pJNK; however, in vivo, CASE in a dose dependent manner decreased the expression of pERK, pJNK as well as pp38.
25934513	8	14	theme	Imp7/8	1739:1744	arg1	phosphorylation					1671:1685	phosphorylation	1671:1685	phosphorylation	1671:1685	Also phosphorylation and subcellular distribution of pSmad2/3, Smad4 and Imp7/8 in TGF-β1-stimulated HSC and HepG2 cells were monitored.
25934513	8	14	theme	Imp7/8	1739:1744	arg1	distribution					1703:1714	subcellular distribution	1691:1714	subcellular distribution	1691:1714	Also phosphorylation and subcellular distribution of pSmad2/3, Smad4 and Imp7/8 in TGF-β1-stimulated HSC and HepG2 cells were monitored.
25934513	7	15	from	activation	1501:1510	arg1	cells					1605:1609	HepG2 cells	1599:1609	HepG2 cells	1599:1609	MATERIALS AND METHODS We used immunofluorescence and western blot techniques to monitor effect of CASE on the activation of the MAPKs (pERK, pJNK and pp38) in TGF-β1-stimulated hepatic stellate cells (HSCs), HepG2 cells and also diethylnitrosamine (DEN)-induced HCC in rats.
25934513	7	15	from	activation	1501:1510	arg1	HSCs					1592:1595	HSCs	1592:1595	HSCs	1592:1595	MATERIALS AND METHODS We used immunofluorescence and western blot techniques to monitor effect of CASE on the activation of the MAPKs (pERK, pJNK and pp38) in TGF-β1-stimulated hepatic stellate cells (HSCs), HepG2 cells and also diethylnitrosamine (DEN)-induced HCC in rats.
25934513	7	15	from	activation	1501:1510	arg1	cells					1585:1589	TGF-β1-stimulated hepatic stellate cells	1550:1589	TGF-β1-stimulated hepatic stellate cells (HSCs)	1550:1596	MATERIALS AND METHODS We used immunofluorescence and western blot techniques to monitor effect of CASE on the activation of the MAPKs (pERK, pJNK and pp38) in TGF-β1-stimulated hepatic stellate cells (HSCs), HepG2 cells and also diethylnitrosamine (DEN)-induced HCC in rats.
25934513	4	16	theme	Salvia	899:904	arg1	miltiorrhiza					906:917	Salvia miltiorrhiza	899:917	Salvia miltiorrhiza	899:917	Previously, we have reported that Compound Astragalus and Salvia miltiorrhiza extract (CASE), a synergized composite extract from Astragalus membranaceus and Salvia miltiorrhiza ameliorates liver fibrosis and hepatocellular carcinoma (HCC) by modulating the TGF-β/Smad pathway.
25934513	11	17	theme	gene	2305:2308	arg1	expression					2310:2319	PAI-1 gene expression	2299:2319	PAI-1 gene expression	2299:2319	And the expression of Imp7/8 and their subcellular distribution were assessed by both immunofluorescence and western blot techniques, while PAI-1 gene expression was assessed by western blot RESULTS In vitro, CASE in a concentration-dependent manner increased the expression of pp38 but decreased the expression of pERK and pJNK; however, in vivo, CASE in a dose dependent manner decreased the expression of pERK, pJNK as well as pp38.
25934513	10	18	theme	subcellular	1976:1986	arg1	distribution					1988:1999	their subcellular distribution	1970:1999	their subcellular distribution	1970:1999	The effect of CASE on domain-specific phosphorylation of Smad2/3 and their subcellular distribution, and the expression of Smad4 and its subcellular distribution in TGF-β1-stimulated HSCs and HepG2 cells were evaluated by using immunofluorescence technique.
25934513	8	19	theme	TGF-β1-stimulated	1749:1765	arg1	HSC					1767:1769	TGF-β1-stimulated HSC	1749:1769	TGF-β1-stimulated HSC	1749:1769	Also phosphorylation and subcellular distribution of pSmad2/3, Smad4 and Imp7/8 in TGF-β1-stimulated HSC and HepG2 cells were monitored.
25934513	12	20	theme	nuclear	2686:2692	arg1	import					2694:2699	their nuclear import	2680:2699	their nuclear import	2680:2699	Also, CASE concentration dependently inhibited pSmad2C/L, pSmad3L, Smad4, Imp7/8 and their nuclear import; it had no effect on pSmad3C in HepG2 cells; significantly decreased PAI-1 gene expression in both in vitro and in vivo.
25934513	11	21	theme	blot	2345:2348	arg1	RESULTS					2350:2356	western blot RESULTS	2337:2356	western blot RESULTS	2337:2356	And the expression of Imp7/8 and their subcellular distribution were assessed by both immunofluorescence and western blot techniques, while PAI-1 gene expression was assessed by western blot RESULTS In vitro, CASE in a concentration-dependent manner increased the expression of pp38 but decreased the expression of pERK and pJNK; however, in vivo, CASE in a dose dependent manner decreased the expression of pERK, pJNK as well as pp38.
25934513	7	22	theme	western	1444:1450	arg1	techniques					1457:1466	immunofluorescence and western blot techniques	1421:1466	techniques	1457:1466	MATERIALS AND METHODS We used immunofluorescence and western blot techniques to monitor effect of CASE on the activation of the MAPKs (pERK, pJNK and pp38) in TGF-β1-stimulated hepatic stellate cells (HSCs), HepG2 cells and also diethylnitrosamine (DEN)-induced HCC in rats.
25934513	11	23	theme	Imp7/8	2181:2186	arg1	expression					2167:2176	the expression	2163:2176	the expression of Imp7/8	2163:2186	And the expression of Imp7/8 and their subcellular distribution were assessed by both immunofluorescence and western blot techniques, while PAI-1 gene expression was assessed by western blot RESULTS In vitro, CASE in a concentration-dependent manner increased the expression of pp38 but decreased the expression of pERK and pJNK; however, in vivo, CASE in a dose dependent manner decreased the expression of pERK, pJNK as well as pp38.
25934513	11	23	theme	Imp7/8	2181:2186	arg1	distribution					2210:2221	their subcellular distribution	2192:2221	their subcellular distribution	2192:2221	And the expression of Imp7/8 and their subcellular distribution were assessed by both immunofluorescence and western blot techniques, while PAI-1 gene expression was assessed by western blot RESULTS In vitro, CASE in a concentration-dependent manner increased the expression of pp38 but decreased the expression of pERK and pJNK; however, in vivo, CASE in a dose dependent manner decreased the expression of pERK, pJNK as well as pp38.
25934513	5	24	theme	overall	1153:1159	arg1	role					1171:1174	overall oncogenic role	1153:1174	overall oncogenic role of TGF-β/Smad signaling in HCC	1153:1205	Meanwhile, MAPK activation and MAPK-dependent linker phosphorylation of Smad2/3 and their preferential nuclear import are crucial for overall oncogenic role of TGF-β/Smad signaling in HCC.
25934513	5	25	from	role	1171:1174	arg1	HCC					1203:1205	HCC	1203:1205	HCC	1203:1205	Meanwhile, MAPK activation and MAPK-dependent linker phosphorylation of Smad2/3 and their preferential nuclear import are crucial for overall oncogenic role of TGF-β/Smad signaling in HCC.
25934513	13	26	theme	expression	3026:3035	arg1	down-regulation					2996:3010	significant down-regulation	2984:3010	significant down-regulation of PAI-1 gene expression	2984:3035	CONCLUSIONS CASE blocked MAPK activation, MAPK-dependent linker phosphorylation of Smad2/3, Smad4 expression, Imp7 expression and their nuclear import leading to significant down-regulation of PAI-1 gene expression; further highlighting the multi-target anti-HCC effect of CASE and its potential drug candidature.
25934513	13	27	theme	PAI-1	3015:3019	arg1	expression					3026:3035	PAI-1 gene expression	3015:3035	PAI-1 gene expression	3015:3035	CONCLUSIONS CASE blocked MAPK activation, MAPK-dependent linker phosphorylation of Smad2/3, Smad4 expression, Imp7 expression and their nuclear import leading to significant down-regulation of PAI-1 gene expression; further highlighting the multi-target anti-HCC effect of CASE and its potential drug candidature.
25934513	4	28	theme	hepatocellular	950:963	arg1	carcinoma					965:973	hepatocellular carcinoma	950:973	hepatocellular carcinoma (HCC)	950:979	Previously, we have reported that Compound Astragalus and Salvia miltiorrhiza extract (CASE), a synergized composite extract from Astragalus membranaceus and Salvia miltiorrhiza ameliorates liver fibrosis and hepatocellular carcinoma (HCC) by modulating the TGF-β/Smad pathway.
25934513	4	28	theme	hepatocellular	950:963	arg1	HCC					976:978	HCC	976:978	HCC	976:978	Previously, we have reported that Compound Astragalus and Salvia miltiorrhiza extract (CASE), a synergized composite extract from Astragalus membranaceus and Salvia miltiorrhiza ameliorates liver fibrosis and hepatocellular carcinoma (HCC) by modulating the TGF-β/Smad pathway.
25934513	7	29	theme	immunofluorescence	1421:1438	arg1	techniques					1457:1466	immunofluorescence and western blot techniques	1421:1466	techniques	1457:1466	MATERIALS AND METHODS We used immunofluorescence and western blot techniques to monitor effect of CASE on the activation of the MAPKs (pERK, pJNK and pp38) in TGF-β1-stimulated hepatic stellate cells (HSCs), HepG2 cells and also diethylnitrosamine (DEN)-induced HCC in rats.
25934513	11	30	theme	pERK	2474:2477	arg1	expression					2460:2469	the expression	2456:2469	the expression of pERK and pJNK	2456:2486	And the expression of Imp7/8 and their subcellular distribution were assessed by both immunofluorescence and western blot techniques, while PAI-1 gene expression was assessed by western blot RESULTS In vitro, CASE in a concentration-dependent manner increased the expression of pp38 but decreased the expression of pERK and pJNK; however, in vivo, CASE in a dose dependent manner decreased the expression of pERK, pJNK as well as pp38.
25934513	6	31	theme	animal	1369:1374	arg1	models					1376:1381	both cell and animal models	1355:1381	models	1376:1381	To elucidate further, we studied the effect of CASE on the MAPK pathway and how it affects MAPK-dependent regulation of TGF-β/Smad signaling using both cell and animal models of HCC.
25934513	5	32	theme	preferential	1109:1120	arg1	import					1130:1135	their preferential nuclear import	1103:1135	their preferential nuclear import	1103:1135	Meanwhile, MAPK activation and MAPK-dependent linker phosphorylation of Smad2/3 and their preferential nuclear import are crucial for overall oncogenic role of TGF-β/Smad signaling in HCC.
25934513	1	33	theme	ETHNOPHARMACOLOGICAL	153:172	arg1	Lamiaceae					259:267	Lamiaceae	259:267	Lamiaceae	259:267	ETHNOPHARMACOLOGICAL RELEVANCE Astragalus membranaceus Bunge (Leguminosae) and Salvia miltiorrhiza Bunge (Lamiaceae) are two important Chinese herbs with a long history of extensive ethnobotanical usage in the treatment of liver-related diseases over many centuries.
25934513	1	33	theme	ETHNOPHARMACOLOGICAL	153:172	arg1	herbs					296:300	two important Chinese herbs	274:300	two important Chinese herbs with a long history of extensive ethnobotanical usage in the treatment of liver-related diseases over many centuries	274:417	ETHNOPHARMACOLOGICAL RELEVANCE Astragalus membranaceus Bunge (Leguminosae) and Salvia miltiorrhiza Bunge (Lamiaceae) are two important Chinese herbs with a long history of extensive ethnobotanical usage in the treatment of liver-related diseases over many centuries.
25934513	1	33	theme	ETHNOPHARMACOLOGICAL	153:172	arg1	membranaceus					195:206	ETHNOPHARMACOLOGICAL RELEVANCE Astragalus membranaceus	153:206	ETHNOPHARMACOLOGICAL RELEVANCE Astragalus membranaceus Bunge (Leguminosae) and Salvia miltiorrhiza Bunge (Lamiaceae)	153:268	ETHNOPHARMACOLOGICAL RELEVANCE Astragalus membranaceus Bunge (Leguminosae) and Salvia miltiorrhiza Bunge (Lamiaceae) are two important Chinese herbs with a long history of extensive ethnobotanical usage in the treatment of liver-related diseases over many centuries.
25934513	1	33	theme	ETHNOPHARMACOLOGICAL	153:172	arg1	Leguminosae					215:225	Leguminosae	215:225	Leguminosae	215:225	ETHNOPHARMACOLOGICAL RELEVANCE Astragalus membranaceus Bunge (Leguminosae) and Salvia miltiorrhiza Bunge (Lamiaceae) are two important Chinese herbs with a long history of extensive ethnobotanical usage in the treatment of liver-related diseases over many centuries.
25934513	11	34	from	CASE	2507:2510	arg1	manner					2532:2537	a dose dependent manner	2515:2537	a dose dependent manner	2515:2537	And the expression of Imp7/8 and their subcellular distribution were assessed by both immunofluorescence and western blot techniques, while PAI-1 gene expression was assessed by western blot RESULTS In vitro, CASE in a concentration-dependent manner increased the expression of pp38 but decreased the expression of pERK and pJNK; however, in vivo, CASE in a dose dependent manner decreased the expression of pERK, pJNK as well as pp38.
25934513	6	35	theme	MAPK	1267:1270	arg1	pathway					1272:1278	the MAPK pathway	1263:1278	the MAPK pathway	1263:1278	To elucidate further, we studied the effect of CASE on the MAPK pathway and how it affects MAPK-dependent regulation of TGF-β/Smad signaling using both cell and animal models of HCC.
25934513	3	36	from	studies	590:596	arg1	herbs					611:615	these two herbs	601:615	these two herbs	601:615	In response, recent studies on these two herbs have focused on elucidating their mechanisms of action, particularly with regards to their anti-hepatocarcinogenic effects.
25934513	6	37	theme	cell	1360:1363	arg1	models					1376:1381	both cell and animal models	1355:1381	models	1376:1381	To elucidate further, we studied the effect of CASE on the MAPK pathway and how it affects MAPK-dependent regulation of TGF-β/Smad signaling using both cell and animal models of HCC.
25934513	1	38	theme	Astragalus	184:193	arg1	Lamiaceae					259:267	Lamiaceae	259:267	Lamiaceae	259:267	ETHNOPHARMACOLOGICAL RELEVANCE Astragalus membranaceus Bunge (Leguminosae) and Salvia miltiorrhiza Bunge (Lamiaceae) are two important Chinese herbs with a long history of extensive ethnobotanical usage in the treatment of liver-related diseases over many centuries.
25934513	1	38	theme	Astragalus	184:193	arg1	herbs					296:300	two important Chinese herbs	274:300	two important Chinese herbs with a long history of extensive ethnobotanical usage in the treatment of liver-related diseases over many centuries	274:417	ETHNOPHARMACOLOGICAL RELEVANCE Astragalus membranaceus Bunge (Leguminosae) and Salvia miltiorrhiza Bunge (Lamiaceae) are two important Chinese herbs with a long history of extensive ethnobotanical usage in the treatment of liver-related diseases over many centuries.
25934513	1	38	theme	Astragalus	184:193	arg1	membranaceus					195:206	ETHNOPHARMACOLOGICAL RELEVANCE Astragalus membranaceus	153:206	ETHNOPHARMACOLOGICAL RELEVANCE Astragalus membranaceus Bunge (Leguminosae) and Salvia miltiorrhiza Bunge (Lamiaceae)	153:268	ETHNOPHARMACOLOGICAL RELEVANCE Astragalus membranaceus Bunge (Leguminosae) and Salvia miltiorrhiza Bunge (Lamiaceae) are two important Chinese herbs with a long history of extensive ethnobotanical usage in the treatment of liver-related diseases over many centuries.
25934513	1	38	theme	Astragalus	184:193	arg1	Leguminosae					215:225	Leguminosae	215:225	Leguminosae	215:225	ETHNOPHARMACOLOGICAL RELEVANCE Astragalus membranaceus Bunge (Leguminosae) and Salvia miltiorrhiza Bunge (Lamiaceae) are two important Chinese herbs with a long history of extensive ethnobotanical usage in the treatment of liver-related diseases over many centuries.
25934513	13	39	theme	significant	2984:2994	arg1	down-regulation					2996:3010	significant down-regulation	2984:3010	significant down-regulation of PAI-1 gene expression	2984:3035	CONCLUSIONS CASE blocked MAPK activation, MAPK-dependent linker phosphorylation of Smad2/3, Smad4 expression, Imp7 expression and their nuclear import leading to significant down-regulation of PAI-1 gene expression; further highlighting the multi-target anti-HCC effect of CASE and its potential drug candidature.
25934513	12	40	theme	CASE	2601:2604	arg1	concentration					2606:2618	CASE concentration	2601:2618	CASE concentration	2601:2618	Also, CASE concentration dependently inhibited pSmad2C/L, pSmad3L, Smad4, Imp7/8 and their nuclear import; it had no effect on pSmad3C in HepG2 cells; significantly decreased PAI-1 gene expression in both in vitro and in vivo.
25934513	0	41	theme	TGF-β/Smad	77:86	arg1	signaling					88:96	MAPK-regulated TGF-β/Smad signaling	62:96	MAPK-regulated TGF-β/Smad signaling in hepatocellular carcinoma	62:124	Compound Astragalus and Salvia miltiorrhiza extracts modulate MAPK-regulated TGF-β/Smad signaling in hepatocellular carcinoma by multi-target mechanism.
25934513	6	42	theme	signaling	1339:1347	arg1	regulation					1314:1323	MAPK-dependent regulation	1299:1323	MAPK-dependent regulation of TGF-β/Smad signaling using both cell and animal models of HCC	1299:1388	To elucidate further, we studied the effect of CASE on the MAPK pathway and how it affects MAPK-dependent regulation of TGF-β/Smad signaling using both cell and animal models of HCC.
25934513	11	43	theme	pJNK	2483:2486	arg1	expression					2460:2469	the expression	2456:2469	the expression of pERK and pJNK	2456:2486	And the expression of Imp7/8 and their subcellular distribution were assessed by both immunofluorescence and western blot techniques, while PAI-1 gene expression was assessed by western blot RESULTS In vitro, CASE in a concentration-dependent manner increased the expression of pp38 but decreased the expression of pERK and pJNK; however, in vivo, CASE in a dose dependent manner decreased the expression of pERK, pJNK as well as pp38.
25934513	7	44	used	used	1416:1419	arg2	MATERIALS					1391:1399	MATERIALS	1391:1399	MATERIALS	1391:1399	MATERIALS AND METHODS We used immunofluorescence and western blot techniques to monitor effect of CASE on the activation of the MAPKs (pERK, pJNK and pp38) in TGF-β1-stimulated hepatic stellate cells (HSCs), HepG2 cells and also diethylnitrosamine (DEN)-induced HCC in rats.
25934513	7	44	used	used	1416:1419	arg2	METHODS					1405:1411	METHODS	1405:1411	METHODS	1405:1411	MATERIALS AND METHODS We used immunofluorescence and western blot techniques to monitor effect of CASE on the activation of the MAPKs (pERK, pJNK and pp38) in TGF-β1-stimulated hepatic stellate cells (HSCs), HepG2 cells and also diethylnitrosamine (DEN)-induced HCC in rats.
25934513	3	45	with	mechanisms	651:660	arg1	regards					691:697	regards	691:697	regards to their anti-hepatocarcinogenic effects	691:738	In response, recent studies on these two herbs have focused on elucidating their mechanisms of action, particularly with regards to their anti-hepatocarcinogenic effects.
25934513	1	46	theme	diseases	390:397	arg1	treatment					363:371	the treatment	359:371	the treatment of liver-related diseases over many centuries	359:417	ETHNOPHARMACOLOGICAL RELEVANCE Astragalus membranaceus Bunge (Leguminosae) and Salvia miltiorrhiza Bunge (Lamiaceae) are two important Chinese herbs with a long history of extensive ethnobotanical usage in the treatment of liver-related diseases over many centuries.
25934513	2	47	dep	formulation	488:498	arg1	either					462:467	either	462:467	either	462:467	Presently, these two herbs are being used either as a single herbal formulation or a composite formula for the treatment of liver related conditions.
25934513	2	48	theme	conditions	558:567	arg1	treatment					531:539	the treatment	527:539	the treatment of liver related conditions	527:567	Presently, these two herbs are being used either as a single herbal formulation or a composite formula for the treatment of liver related conditions.
25934513	4	49	theme	miltiorrhiza	806:817	arg1	extract					819:825	Salvia miltiorrhiza extract	799:825	Salvia miltiorrhiza extract	799:825	Previously, we have reported that Compound Astragalus and Salvia miltiorrhiza extract (CASE), a synergized composite extract from Astragalus membranaceus and Salvia miltiorrhiza ameliorates liver fibrosis and hepatocellular carcinoma (HCC) by modulating the TGF-β/Smad pathway.
25934513	10	50	theme	subcellular	2038:2048	arg1	distribution					2050:2061	its subcellular distribution	2034:2061	its subcellular distribution	2034:2061	The effect of CASE on domain-specific phosphorylation of Smad2/3 and their subcellular distribution, and the expression of Smad4 and its subcellular distribution in TGF-β1-stimulated HSCs and HepG2 cells were evaluated by using immunofluorescence technique.
25934513	1	51	theme	many	404:407	arg1	centuries					409:417	many centuries	404:417	many centuries	404:417	ETHNOPHARMACOLOGICAL RELEVANCE Astragalus membranaceus Bunge (Leguminosae) and Salvia miltiorrhiza Bunge (Lamiaceae) are two important Chinese herbs with a long history of extensive ethnobotanical usage in the treatment of liver-related diseases over many centuries.
25934513	2	52	theme	liver	544:548	arg1	conditions					558:567	liver related conditions	544:567	liver related conditions	544:567	Presently, these two herbs are being used either as a single herbal formulation or a composite formula for the treatment of liver related conditions.
25934513	0	53	theme	multi-target	129:140	arg1	mechanism					142:150	multi-target mechanism	129:150	multi-target mechanism	129:150	Compound Astragalus and Salvia miltiorrhiza extracts modulate MAPK-regulated TGF-β/Smad signaling in hepatocellular carcinoma by multi-target mechanism.
25934513	4	54	from	membranaceus	882:893	arg1	Astragalus					784:793	Compound Astragalus	775:793	Compound Astragalus	775:793	Previously, we have reported that Compound Astragalus and Salvia miltiorrhiza extract (CASE), a synergized composite extract from Astragalus membranaceus and Salvia miltiorrhiza ameliorates liver fibrosis and hepatocellular carcinoma (HCC) by modulating the TGF-β/Smad pathway.
25934513	4	54	from	membranaceus	882:893	arg1	extract					858:864	a synergized composite extract	835:864	a synergized composite extract from Astragalus membranaceus	835:893	Previously, we have reported that Compound Astragalus and Salvia miltiorrhiza extract (CASE), a synergized composite extract from Astragalus membranaceus and Salvia miltiorrhiza ameliorates liver fibrosis and hepatocellular carcinoma (HCC) by modulating the TGF-β/Smad pathway.
25934513	4	54	from	membranaceus	882:893	arg1	CASE					828:831	CASE	828:831	CASE	828:831	Previously, we have reported that Compound Astragalus and Salvia miltiorrhiza extract (CASE), a synergized composite extract from Astragalus membranaceus and Salvia miltiorrhiza ameliorates liver fibrosis and hepatocellular carcinoma (HCC) by modulating the TGF-β/Smad pathway.
25934513	4	54	from	membranaceus	882:893	arg1	extract					819:825	Salvia miltiorrhiza extract	799:825	Salvia miltiorrhiza extract	799:825	Previously, we have reported that Compound Astragalus and Salvia miltiorrhiza extract (CASE), a synergized composite extract from Astragalus membranaceus and Salvia miltiorrhiza ameliorates liver fibrosis and hepatocellular carcinoma (HCC) by modulating the TGF-β/Smad pathway.
25934513	4	54	from	membranaceus	882:893	arg1	miltiorrhiza					906:917	Salvia miltiorrhiza	899:917	Salvia miltiorrhiza	899:917	Previously, we have reported that Compound Astragalus and Salvia miltiorrhiza extract (CASE), a synergized composite extract from Astragalus membranaceus and Salvia miltiorrhiza ameliorates liver fibrosis and hepatocellular carcinoma (HCC) by modulating the TGF-β/Smad pathway.
25934513	7	55	from	HCC	1653:1655	arg1	rats					1660:1663	rats	1660:1663	rats	1660:1663	MATERIALS AND METHODS We used immunofluorescence and western blot techniques to monitor effect of CASE on the activation of the MAPKs (pERK, pJNK and pp38) in TGF-β1-stimulated hepatic stellate cells (HSCs), HepG2 cells and also diethylnitrosamine (DEN)-induced HCC in rats.
25934513	2	56	theme	herbal	481:486	arg1	herbs					441:445	these two herbs	431:445	these two herbs	431:445	Presently, these two herbs are being used either as a single herbal formulation or a composite formula for the treatment of liver related conditions.
25934513	2	56	theme	herbal	481:486	arg1	formulation					488:498	a single herbal formulation	472:498	a single herbal formulation	472:498	Presently, these two herbs are being used either as a single herbal formulation or a composite formula for the treatment of liver related conditions.
25934513	11	57	dep	assessed	2228:2235	arg1	decreased					2539:2547	decreased	2539:2547	decreased the expression of pERK, pJNK as well as pp38	2539:2592	And the expression of Imp7/8 and their subcellular distribution were assessed by both immunofluorescence and western blot techniques, while PAI-1 gene expression was assessed by western blot RESULTS In vitro, CASE in a concentration-dependent manner increased the expression of pp38 but decreased the expression of pERK and pJNK; however, in vivo, CASE in a dose dependent manner decreased the expression of pERK, pJNK as well as pp38.
25934513	11	57	dep	assessed	2228:2235	arg1	increased					2409:2417	increased	2409:2417	increased the expression of pp38	2409:2440	And the expression of Imp7/8 and their subcellular distribution were assessed by both immunofluorescence and western blot techniques, while PAI-1 gene expression was assessed by western blot RESULTS In vitro, CASE in a concentration-dependent manner increased the expression of pp38 but decreased the expression of pERK and pJNK; however, in vivo, CASE in a dose dependent manner decreased the expression of pERK, pJNK as well as pp38.
25934513	11	57	dep	assessed	2228:2235	arg1	decreased					2446:2454	decreased	2446:2454	decreased the expression of pERK and pJNK	2446:2486	And the expression of Imp7/8 and their subcellular distribution were assessed by both immunofluorescence and western blot techniques, while PAI-1 gene expression was assessed by western blot RESULTS In vitro, CASE in a concentration-dependent manner increased the expression of pp38 but decreased the expression of pERK and pJNK; however, in vivo, CASE in a dose dependent manner decreased the expression of pERK, pJNK as well as pp38.
25934513	4	58	theme	Compound	775:782	arg1	Astragalus					784:793	Compound Astragalus	775:793	Compound Astragalus	775:793	Previously, we have reported that Compound Astragalus and Salvia miltiorrhiza extract (CASE), a synergized composite extract from Astragalus membranaceus and Salvia miltiorrhiza ameliorates liver fibrosis and hepatocellular carcinoma (HCC) by modulating the TGF-β/Smad pathway.
25934513	4	58	theme	Compound	775:782	arg1	CASE					828:831	CASE	828:831	CASE	828:831	Previously, we have reported that Compound Astragalus and Salvia miltiorrhiza extract (CASE), a synergized composite extract from Astragalus membranaceus and Salvia miltiorrhiza ameliorates liver fibrosis and hepatocellular carcinoma (HCC) by modulating the TGF-β/Smad pathway.
25934513	6	59	from	effect	1245:1250	arg1	pathway					1272:1278	the MAPK pathway	1263:1278	the MAPK pathway	1263:1278	To elucidate further, we studied the effect of CASE on the MAPK pathway and how it affects MAPK-dependent regulation of TGF-β/Smad signaling using both cell and animal models of HCC.
25934513	0	60	theme	Compound	0:7	arg1	Astragalus					9:18	Compound Astragalus	0:18	Compound Astragalus	0:18	Compound Astragalus and Salvia miltiorrhiza extracts modulate MAPK-regulated TGF-β/Smad signaling in hepatocellular carcinoma by multi-target mechanism.
25934513	9	61	theme	pJNK	1827:1830	arg1	expression					1807:1816	The expression	1803:1816	The expression of pERK, pJNK, pp38 and PAI-1 gene	1803:1851	The expression of pERK, pJNK, pp38 and PAI-1 gene were monitored by using western blot technique.
25934513	11	62	theme	pp38	2437:2440	arg1	expression					2423:2432	the expression	2419:2432	the expression of pp38	2419:2440	And the expression of Imp7/8 and their subcellular distribution were assessed by both immunofluorescence and western blot techniques, while PAI-1 gene expression was assessed by western blot RESULTS In vitro, CASE in a concentration-dependent manner increased the expression of pp38 but decreased the expression of pERK and pJNK; however, in vivo, CASE in a dose dependent manner decreased the expression of pERK, pJNK as well as pp38.
25934513	1	63	dep	membranaceus	195:206	arg1	Lamiaceae					259:267	Lamiaceae	259:267	Lamiaceae	259:267	ETHNOPHARMACOLOGICAL RELEVANCE Astragalus membranaceus Bunge (Leguminosae) and Salvia miltiorrhiza Bunge (Lamiaceae) are two important Chinese herbs with a long history of extensive ethnobotanical usage in the treatment of liver-related diseases over many centuries.
25934513	1	63	dep	membranaceus	195:206	arg1	membranaceus					195:206	ETHNOPHARMACOLOGICAL RELEVANCE Astragalus membranaceus	153:206	ETHNOPHARMACOLOGICAL RELEVANCE Astragalus membranaceus Bunge (Leguminosae) and Salvia miltiorrhiza Bunge (Lamiaceae)	153:268	ETHNOPHARMACOLOGICAL RELEVANCE Astragalus membranaceus Bunge (Leguminosae) and Salvia miltiorrhiza Bunge (Lamiaceae) are two important Chinese herbs with a long history of extensive ethnobotanical usage in the treatment of liver-related diseases over many centuries.
25934513	1	63	dep	membranaceus	195:206	arg1	Leguminosae					215:225	Leguminosae	215:225	Leguminosae	215:225	ETHNOPHARMACOLOGICAL RELEVANCE Astragalus membranaceus Bunge (Leguminosae) and Salvia miltiorrhiza Bunge (Lamiaceae) are two important Chinese herbs with a long history of extensive ethnobotanical usage in the treatment of liver-related diseases over many centuries.
25934513	1	64	theme	extensive	325:333	arg1	usage					350:354	extensive ethnobotanical usage	325:354	extensive ethnobotanical usage in the treatment of liver-related diseases over many centuries	325:417	ETHNOPHARMACOLOGICAL RELEVANCE Astragalus membranaceus Bunge (Leguminosae) and Salvia miltiorrhiza Bunge (Lamiaceae) are two important Chinese herbs with a long history of extensive ethnobotanical usage in the treatment of liver-related diseases over many centuries.
25934513	10	65	from	expression	2010:2019	arg1	HSCs					2084:2087	TGF-β1-stimulated HSCs	2066:2087	TGF-β1-stimulated HSCs	2066:2087	The effect of CASE on domain-specific phosphorylation of Smad2/3 and their subcellular distribution, and the expression of Smad4 and its subcellular distribution in TGF-β1-stimulated HSCs and HepG2 cells were evaluated by using immunofluorescence technique.
25934513	10	65	from	expression	2010:2019	arg1	cells					2099:2103	HepG2 cells	2093:2103	HepG2 cells	2093:2103	The effect of CASE on domain-specific phosphorylation of Smad2/3 and their subcellular distribution, and the expression of Smad4 and its subcellular distribution in TGF-β1-stimulated HSCs and HepG2 cells were evaluated by using immunofluorescence technique.
25934513	7	66	theme	stellate	1576:1583	arg1	cells					1605:1609	HepG2 cells	1599:1609	HepG2 cells	1599:1609	MATERIALS AND METHODS We used immunofluorescence and western blot techniques to monitor effect of CASE on the activation of the MAPKs (pERK, pJNK and pp38) in TGF-β1-stimulated hepatic stellate cells (HSCs), HepG2 cells and also diethylnitrosamine (DEN)-induced HCC in rats.
25934513	7	66	theme	stellate	1576:1583	arg1	HSCs					1592:1595	HSCs	1592:1595	HSCs	1592:1595	MATERIALS AND METHODS We used immunofluorescence and western blot techniques to monitor effect of CASE on the activation of the MAPKs (pERK, pJNK and pp38) in TGF-β1-stimulated hepatic stellate cells (HSCs), HepG2 cells and also diethylnitrosamine (DEN)-induced HCC in rats.
25934513	7	66	theme	stellate	1576:1583	arg1	cells					1585:1589	TGF-β1-stimulated hepatic stellate cells	1550:1589	TGF-β1-stimulated hepatic stellate cells (HSCs)	1550:1596	MATERIALS AND METHODS We used immunofluorescence and western blot techniques to monitor effect of CASE on the activation of the MAPKs (pERK, pJNK and pp38) in TGF-β1-stimulated hepatic stellate cells (HSCs), HepG2 cells and also diethylnitrosamine (DEN)-induced HCC in rats.
25934513	13	67	theme	candidature	3123:3133	arg1	effect					3085:3090	the multi-target anti-HCC effect	3059:3090	the multi-target anti-HCC effect of CASE and its potential drug candidature	3059:3133	CONCLUSIONS CASE blocked MAPK activation, MAPK-dependent linker phosphorylation of Smad2/3, Smad4 expression, Imp7 expression and their nuclear import leading to significant down-regulation of PAI-1 gene expression; further highlighting the multi-target anti-HCC effect of CASE and its potential drug candidature.
25934513	2	68	theme	composite	505:513	arg1	formula					515:521	a composite formula	503:521	a composite formula for the treatment of liver related conditions	503:567	Presently, these two herbs are being used either as a single herbal formulation or a composite formula for the treatment of liver related conditions.
25934513	2	68	theme	composite	505:513	arg1	herbs					441:445	these two herbs	431:445	these two herbs	431:445	Presently, these two herbs are being used either as a single herbal formulation or a composite formula for the treatment of liver related conditions.
25934513	12	69	theme	gene	2776:2779	arg1	expression					2781:2790	PAI-1 gene expression	2770:2790	PAI-1 gene expression	2770:2790	Also, CASE concentration dependently inhibited pSmad2C/L, pSmad3L, Smad4, Imp7/8 and their nuclear import; it had no effect on pSmad3C in HepG2 cells; significantly decreased PAI-1 gene expression in both in vitro and in vivo.
25934513	4	70	theme	composite	848:856	arg1	extract					858:864	a synergized composite extract	835:864	a synergized composite extract from Astragalus membranaceus	835:893	Previously, we have reported that Compound Astragalus and Salvia miltiorrhiza extract (CASE), a synergized composite extract from Astragalus membranaceus and Salvia miltiorrhiza ameliorates liver fibrosis and hepatocellular carcinoma (HCC) by modulating the TGF-β/Smad pathway.
25934513	0	71	theme	miltiorrhiza	31:42	arg1	extracts					44:51	Salvia miltiorrhiza extracts	24:51	Salvia miltiorrhiza extracts	24:51	Compound Astragalus and Salvia miltiorrhiza extracts modulate MAPK-regulated TGF-β/Smad signaling in hepatocellular carcinoma by multi-target mechanism.
25934513	1	72	theme	usage	350:354	arg1	history					314:320	a long history	307:320	a long history of extensive ethnobotanical usage in the treatment of liver-related diseases over many centuries	307:417	ETHNOPHARMACOLOGICAL RELEVANCE Astragalus membranaceus Bunge (Leguminosae) and Salvia miltiorrhiza Bunge (Lamiaceae) are two important Chinese herbs with a long history of extensive ethnobotanical usage in the treatment of liver-related diseases over many centuries.
25934513	7	73	theme	TGF-β1-stimulated	1550:1566	arg1	cells					1605:1609	HepG2 cells	1599:1609	HepG2 cells	1599:1609	MATERIALS AND METHODS We used immunofluorescence and western blot techniques to monitor effect of CASE on the activation of the MAPKs (pERK, pJNK and pp38) in TGF-β1-stimulated hepatic stellate cells (HSCs), HepG2 cells and also diethylnitrosamine (DEN)-induced HCC in rats.
25934513	7	73	theme	TGF-β1-stimulated	1550:1566	arg1	HSCs					1592:1595	HSCs	1592:1595	HSCs	1592:1595	MATERIALS AND METHODS We used immunofluorescence and western blot techniques to monitor effect of CASE on the activation of the MAPKs (pERK, pJNK and pp38) in TGF-β1-stimulated hepatic stellate cells (HSCs), HepG2 cells and also diethylnitrosamine (DEN)-induced HCC in rats.
25934513	7	73	theme	TGF-β1-stimulated	1550:1566	arg1	cells					1585:1589	TGF-β1-stimulated hepatic stellate cells	1550:1589	TGF-β1-stimulated hepatic stellate cells (HSCs)	1550:1596	MATERIALS AND METHODS We used immunofluorescence and western blot techniques to monitor effect of CASE on the activation of the MAPKs (pERK, pJNK and pp38) in TGF-β1-stimulated hepatic stellate cells (HSCs), HepG2 cells and also diethylnitrosamine (DEN)-induced HCC in rats.
25934513	5	74	theme	Smad2/3	1091:1097	arg1	import					1130:1135	their preferential nuclear import	1103:1135	their preferential nuclear import	1103:1135	Meanwhile, MAPK activation and MAPK-dependent linker phosphorylation of Smad2/3 and their preferential nuclear import are crucial for overall oncogenic role of TGF-β/Smad signaling in HCC.
25934513	5	74	theme	Smad2/3	1091:1097	arg1	phosphorylation					1072:1086	MAPK-dependent linker phosphorylation	1050:1086	MAPK-dependent linker phosphorylation of Smad2/3	1050:1097	Meanwhile, MAPK activation and MAPK-dependent linker phosphorylation of Smad2/3 and their preferential nuclear import are crucial for overall oncogenic role of TGF-β/Smad signaling in HCC.
25934513	5	74	theme	Smad2/3	1091:1097	arg1	Meanwhile					1019:1027	Meanwhile	1019:1027	Meanwhile	1019:1027	Meanwhile, MAPK activation and MAPK-dependent linker phosphorylation of Smad2/3 and their preferential nuclear import are crucial for overall oncogenic role of TGF-β/Smad signaling in HCC.
25934513	5	74	theme	Smad2/3	1091:1097	arg1	activation					1035:1044	MAPK activation	1030:1044	MAPK activation	1030:1044	Meanwhile, MAPK activation and MAPK-dependent linker phosphorylation of Smad2/3 and their preferential nuclear import are crucial for overall oncogenic role of TGF-β/Smad signaling in HCC.
25934513	9	75	theme	PAI-1	1842:1846	arg1	gene					1848:1851	PAI-1 gene	1842:1851	PAI-1 gene	1842:1851	The expression of pERK, pJNK, pp38 and PAI-1 gene were monitored by using western blot technique.
25934513	3	76	theme	action	665:670	arg1	mechanisms					651:660	their mechanisms	645:660	their mechanisms	645:660	In response, recent studies on these two herbs have focused on elucidating their mechanisms of action, particularly with regards to their anti-hepatocarcinogenic effects.
25934513	7	77	dep	MATERIALS	1391:1399	arg1	We					1413:1414	We	1413:1414	We	1413:1414	MATERIALS AND METHODS We used immunofluorescence and western blot techniques to monitor effect of CASE on the activation of the MAPKs (pERK, pJNK and pp38) in TGF-β1-stimulated hepatic stellate cells (HSCs), HepG2 cells and also diethylnitrosamine (DEN)-induced HCC in rats.
25934513	1	78	with	herbs	296:300	arg1	history					314:320	a long history	307:320	a long history of extensive ethnobotanical usage in the treatment of liver-related diseases over many centuries	307:417	ETHNOPHARMACOLOGICAL RELEVANCE Astragalus membranaceus Bunge (Leguminosae) and Salvia miltiorrhiza Bunge (Lamiaceae) are two important Chinese herbs with a long history of extensive ethnobotanical usage in the treatment of liver-related diseases over many centuries.
25934513	13	79	theme	Imp7	2932:2935	arg1	expression					2937:2946	Imp7 expression	2932:2946	Imp7 expression	2932:2946	CONCLUSIONS CASE blocked MAPK activation, MAPK-dependent linker phosphorylation of Smad2/3, Smad4 expression, Imp7 expression and their nuclear import leading to significant down-regulation of PAI-1 gene expression; further highlighting the multi-target anti-HCC effect of CASE and its potential drug candidature.
25934513	10	80	theme	HepG2	2093:2097	arg1	cells					2099:2103	HepG2 cells	2093:2103	HepG2 cells	2093:2103	The effect of CASE on domain-specific phosphorylation of Smad2/3 and their subcellular distribution, and the expression of Smad4 and its subcellular distribution in TGF-β1-stimulated HSCs and HepG2 cells were evaluated by using immunofluorescence technique.
25934513	10	81	from	distribution	2050:2061	arg1	HSCs					2084:2087	TGF-β1-stimulated HSCs	2066:2087	TGF-β1-stimulated HSCs	2066:2087	The effect of CASE on domain-specific phosphorylation of Smad2/3 and their subcellular distribution, and the expression of Smad4 and its subcellular distribution in TGF-β1-stimulated HSCs and HepG2 cells were evaluated by using immunofluorescence technique.
25934513	10	81	from	distribution	2050:2061	arg1	cells					2099:2103	HepG2 cells	2093:2103	HepG2 cells	2093:2103	The effect of CASE on domain-specific phosphorylation of Smad2/3 and their subcellular distribution, and the expression of Smad4 and its subcellular distribution in TGF-β1-stimulated HSCs and HepG2 cells were evaluated by using immunofluorescence technique.
25934513	5	82	theme	MAPK-dependent	1050:1063	arg1	phosphorylation					1072:1086	MAPK-dependent linker phosphorylation	1050:1086	MAPK-dependent linker phosphorylation of Smad2/3	1050:1097	Meanwhile, MAPK activation and MAPK-dependent linker phosphorylation of Smad2/3 and their preferential nuclear import are crucial for overall oncogenic role of TGF-β/Smad signaling in HCC.
25934513	13	83	theme	potential	3108:3116	arg1	candidature					3123:3133	its potential drug candidature	3104:3133	its potential drug candidature	3104:3133	CONCLUSIONS CASE blocked MAPK activation, MAPK-dependent linker phosphorylation of Smad2/3, Smad4 expression, Imp7 expression and their nuclear import leading to significant down-regulation of PAI-1 gene expression; further highlighting the multi-target anti-HCC effect of CASE and its potential drug candidature.
25934513	11	84	theme	PAI-1	2299:2303	arg1	expression					2310:2319	PAI-1 gene expression	2299:2319	PAI-1 gene expression	2299:2319	And the expression of Imp7/8 and their subcellular distribution were assessed by both immunofluorescence and western blot techniques, while PAI-1 gene expression was assessed by western blot RESULTS In vitro, CASE in a concentration-dependent manner increased the expression of pp38 but decreased the expression of pERK and pJNK; however, in vivo, CASE in a dose dependent manner decreased the expression of pERK, pJNK as well as pp38.
25934513	1	85	theme	important	278:286	arg1	membranaceus					195:206	ETHNOPHARMACOLOGICAL RELEVANCE Astragalus membranaceus	153:206	ETHNOPHARMACOLOGICAL RELEVANCE Astragalus membranaceus Bunge (Leguminosae) and Salvia miltiorrhiza Bunge (Lamiaceae)	153:268	ETHNOPHARMACOLOGICAL RELEVANCE Astragalus membranaceus Bunge (Leguminosae) and Salvia miltiorrhiza Bunge (Lamiaceae) are two important Chinese herbs with a long history of extensive ethnobotanical usage in the treatment of liver-related diseases over many centuries.
25934513	1	85	theme	important	278:286	arg1	herbs					296:300	two important Chinese herbs	274:300	two important Chinese herbs with a long history of extensive ethnobotanical usage in the treatment of liver-related diseases over many centuries	274:417	ETHNOPHARMACOLOGICAL RELEVANCE Astragalus membranaceus Bunge (Leguminosae) and Salvia miltiorrhiza Bunge (Lamiaceae) are two important Chinese herbs with a long history of extensive ethnobotanical usage in the treatment of liver-related diseases over many centuries.
25934513	10	86	from	effect	1905:1910	arg1	expression					2010:2019	the expression	2006:2019	the expression of Smad4	2006:2028	The effect of CASE on domain-specific phosphorylation of Smad2/3 and their subcellular distribution, and the expression of Smad4 and its subcellular distribution in TGF-β1-stimulated HSCs and HepG2 cells were evaluated by using immunofluorescence technique.
25934513	10	86	from	effect	1905:1910	arg1	phosphorylation					1939:1953	domain-specific phosphorylation	1923:1953	domain-specific phosphorylation of Smad2/3	1923:1964	The effect of CASE on domain-specific phosphorylation of Smad2/3 and their subcellular distribution, and the expression of Smad4 and its subcellular distribution in TGF-β1-stimulated HSCs and HepG2 cells were evaluated by using immunofluorescence technique.
25934513	10	86	from	effect	1905:1910	arg1	distribution					1988:1999	their subcellular distribution	1970:1999	their subcellular distribution	1970:1999	The effect of CASE on domain-specific phosphorylation of Smad2/3 and their subcellular distribution, and the expression of Smad4 and its subcellular distribution in TGF-β1-stimulated HSCs and HepG2 cells were evaluated by using immunofluorescence technique.
25934513	9	87	theme	western	1877:1883	arg1	technique					1890:1898	western blot technique	1877:1898	western blot technique	1877:1898	The expression of pERK, pJNK, pp38 and PAI-1 gene were monitored by using western blot technique.
25934513	8	88	theme	subcellular	1691:1701	arg1	distribution					1703:1714	subcellular distribution	1691:1714	subcellular distribution	1691:1714	Also phosphorylation and subcellular distribution of pSmad2/3, Smad4 and Imp7/8 in TGF-β1-stimulated HSC and HepG2 cells were monitored.
25934513	13	89	theme	linker	2879:2884	arg1	phosphorylation					2886:2900	MAPK-dependent linker phosphorylation	2864:2900	MAPK-dependent linker phosphorylation of Smad2/3	2864:2911	CONCLUSIONS CASE blocked MAPK activation, MAPK-dependent linker phosphorylation of Smad2/3, Smad4 expression, Imp7 expression and their nuclear import leading to significant down-regulation of PAI-1 gene expression; further highlighting the multi-target anti-HCC effect of CASE and its potential drug candidature.
25934513	1	90	from	history	314:320	arg1	treatment					363:371	the treatment	359:371	the treatment of liver-related diseases over many centuries	359:417	ETHNOPHARMACOLOGICAL RELEVANCE Astragalus membranaceus Bunge (Leguminosae) and Salvia miltiorrhiza Bunge (Lamiaceae) are two important Chinese herbs with a long history of extensive ethnobotanical usage in the treatment of liver-related diseases over many centuries.
25934513	1	91	from	usage	350:354	arg1	treatment					363:371	the treatment	359:371	the treatment of liver-related diseases over many centuries	359:417	ETHNOPHARMACOLOGICAL RELEVANCE Astragalus membranaceus Bunge (Leguminosae) and Salvia miltiorrhiza Bunge (Lamiaceae) are two important Chinese herbs with a long history of extensive ethnobotanical usage in the treatment of liver-related diseases over many centuries.
25934513	11	92	from	CASE	2368:2371	arg1	manner					2402:2407	a concentration-dependent manner	2376:2407	a concentration-dependent manner	2376:2407	And the expression of Imp7/8 and their subcellular distribution were assessed by both immunofluorescence and western blot techniques, while PAI-1 gene expression was assessed by western blot RESULTS In vitro, CASE in a concentration-dependent manner increased the expression of pp38 but decreased the expression of pERK and pJNK; however, in vivo, CASE in a dose dependent manner decreased the expression of pERK, pJNK as well as pp38.
25934513	4	93	theme	liver	931:935	arg1	fibrosis					937:944	liver fibrosis	931:944	liver fibrosis	931:944	Previously, we have reported that Compound Astragalus and Salvia miltiorrhiza extract (CASE), a synergized composite extract from Astragalus membranaceus and Salvia miltiorrhiza ameliorates liver fibrosis and hepatocellular carcinoma (HCC) by modulating the TGF-β/Smad pathway.
25934513	13	94	theme	MAPK	2847:2850	arg1	activation					2852:2861	MAPK activation	2847:2861	MAPK activation	2847:2861	CONCLUSIONS CASE blocked MAPK activation, MAPK-dependent linker phosphorylation of Smad2/3, Smad4 expression, Imp7 expression and their nuclear import leading to significant down-regulation of PAI-1 gene expression; further highlighting the multi-target anti-HCC effect of CASE and its potential drug candidature.
25934513	4	95	theme	Astragalus	871:880	arg1	membranaceus					882:893	Astragalus membranaceus	871:893	Astragalus membranaceus	871:893	Previously, we have reported that Compound Astragalus and Salvia miltiorrhiza extract (CASE), a synergized composite extract from Astragalus membranaceus and Salvia miltiorrhiza ameliorates liver fibrosis and hepatocellular carcinoma (HCC) by modulating the TGF-β/Smad pathway.
25934513	7	96	theme	CASE	1489:1492	arg1	effect					1479:1484	effect	1479:1484	effect of CASE on the activation of the MAPKs (pERK, pJNK and pp38) in TGF-β1-stimulated hepatic stellate cells (HSCs), HepG2 cells	1479:1609	MATERIALS AND METHODS We used immunofluorescence and western blot techniques to monitor effect of CASE on the activation of the MAPKs (pERK, pJNK and pp38) in TGF-β1-stimulated hepatic stellate cells (HSCs), HepG2 cells and also diethylnitrosamine (DEN)-induced HCC in rats.
25934513	10	97	theme	immunofluorescence	2129:2146	arg1	technique					2148:2156	immunofluorescence technique	2129:2156	immunofluorescence technique	2129:2156	The effect of CASE on domain-specific phosphorylation of Smad2/3 and their subcellular distribution, and the expression of Smad4 and its subcellular distribution in TGF-β1-stimulated HSCs and HepG2 cells were evaluated by using immunofluorescence technique.
25934513	6	98	theme	MAPK-dependent	1299:1312	arg1	regulation					1314:1323	MAPK-dependent regulation	1299:1323	MAPK-dependent regulation of TGF-β/Smad signaling using both cell and animal models of HCC	1299:1388	To elucidate further, we studied the effect of CASE on the MAPK pathway and how it affects MAPK-dependent regulation of TGF-β/Smad signaling using both cell and animal models of HCC.
25934513	11	99	theme	western	2337:2343	arg1	RESULTS					2350:2356	western blot RESULTS	2337:2356	western blot RESULTS	2337:2356	And the expression of Imp7/8 and their subcellular distribution were assessed by both immunofluorescence and western blot techniques, while PAI-1 gene expression was assessed by western blot RESULTS In vitro, CASE in a concentration-dependent manner increased the expression of pp38 but decreased the expression of pERK and pJNK; however, in vivo, CASE in a dose dependent manner decreased the expression of pERK, pJNK as well as pp38.
25934513	12	100	contain	had	2705:2707	arg1	it					2702:2703	it	2702:2703	it	2702:2703	Also, CASE concentration dependently inhibited pSmad2C/L, pSmad3L, Smad4, Imp7/8 and their nuclear import; it had no effect on pSmad3C in HepG2 cells; significantly decreased PAI-1 gene expression in both in vitro and in vivo.
25934513	12	100	contain	had	2705:2707	arg2	effect					2712:2717	no effect	2709:2717	no effect	2709:2717	Also, CASE concentration dependently inhibited pSmad2C/L, pSmad3L, Smad4, Imp7/8 and their nuclear import; it had no effect on pSmad3C in HepG2 cells; significantly decreased PAI-1 gene expression in both in vitro and in vivo.
25934513	3	101	theme	recent	583:588	arg1	studies					590:596	recent studies	583:596	recent studies on these two herbs	583:615	In response, recent studies on these two herbs have focused on elucidating their mechanisms of action, particularly with regards to their anti-hepatocarcinogenic effects.
25934513	7	102	theme	-induced	1644:1651	arg1	HCC					1653:1655	diethylnitrosamine (DEN)-induced HCC	1620:1655	diethylnitrosamine (DEN)-induced HCC in rats	1620:1663	MATERIALS AND METHODS We used immunofluorescence and western blot techniques to monitor effect of CASE on the activation of the MAPKs (pERK, pJNK and pp38) in TGF-β1-stimulated hepatic stellate cells (HSCs), HepG2 cells and also diethylnitrosamine (DEN)-induced HCC in rats.
25934513	6	103	dep	studied	1233:1239	arg1	affects					1291:1297	affects	1291:1297	affects MAPK-dependent regulation of TGF-β/Smad signaling using both cell and animal models of HCC	1291:1388	To elucidate further, we studied the effect of CASE on the MAPK pathway and how it affects MAPK-dependent regulation of TGF-β/Smad signaling using both cell and animal models of HCC.
25934513	10	104	theme	Smad2/3	1958:1964	arg1	distribution					2050:2061	its subcellular distribution	2034:2061	its subcellular distribution	2034:2061	The effect of CASE on domain-specific phosphorylation of Smad2/3 and their subcellular distribution, and the expression of Smad4 and its subcellular distribution in TGF-β1-stimulated HSCs and HepG2 cells were evaluated by using immunofluorescence technique.
25934513	10	104	theme	Smad2/3	1958:1964	arg1	expression					2010:2019	the expression	2006:2019	the expression of Smad4	2006:2028	The effect of CASE on domain-specific phosphorylation of Smad2/3 and their subcellular distribution, and the expression of Smad4 and its subcellular distribution in TGF-β1-stimulated HSCs and HepG2 cells were evaluated by using immunofluorescence technique.
25934513	10	104	theme	Smad2/3	1958:1964	arg1	phosphorylation					1939:1953	domain-specific phosphorylation	1923:1953	domain-specific phosphorylation of Smad2/3	1923:1964	The effect of CASE on domain-specific phosphorylation of Smad2/3 and their subcellular distribution, and the expression of Smad4 and its subcellular distribution in TGF-β1-stimulated HSCs and HepG2 cells were evaluated by using immunofluorescence technique.
25934513	10	104	theme	Smad2/3	1958:1964	arg1	distribution					1988:1999	their subcellular distribution	1970:1999	their subcellular distribution	1970:1999	The effect of CASE on domain-specific phosphorylation of Smad2/3 and their subcellular distribution, and the expression of Smad4 and its subcellular distribution in TGF-β1-stimulated HSCs and HepG2 cells were evaluated by using immunofluorescence technique.
25934513	6	105	theme	HCC	1386:1388	arg1	models					1376:1381	both cell and animal models	1355:1381	models	1376:1381	To elucidate further, we studied the effect of CASE on the MAPK pathway and how it affects MAPK-dependent regulation of TGF-β/Smad signaling using both cell and animal models of HCC.
25934513	0	106	from	signaling	88:96	arg1	carcinoma					116:124	hepatocellular carcinoma	101:124	hepatocellular carcinoma	101:124	Compound Astragalus and Salvia miltiorrhiza extracts modulate MAPK-regulated TGF-β/Smad signaling in hepatocellular carcinoma by multi-target mechanism.
25934513	7	107	theme	blot	1452:1455	arg1	techniques					1457:1466	immunofluorescence and western blot techniques	1421:1466	techniques	1457:1466	MATERIALS AND METHODS We used immunofluorescence and western blot techniques to monitor effect of CASE on the activation of the MAPKs (pERK, pJNK and pp38) in TGF-β1-stimulated hepatic stellate cells (HSCs), HepG2 cells and also diethylnitrosamine (DEN)-induced HCC in rats.
25934513	4	108	theme	TGF-β/Smad	999:1008	arg1	pathway					1010:1016	the TGF-β/Smad pathway	995:1016	the TGF-β/Smad pathway	995:1016	Previously, we have reported that Compound Astragalus and Salvia miltiorrhiza extract (CASE), a synergized composite extract from Astragalus membranaceus and Salvia miltiorrhiza ameliorates liver fibrosis and hepatocellular carcinoma (HCC) by modulating the TGF-β/Smad pathway.
25934513	5	109	theme	oncogenic	1161:1169	arg1	role					1171:1174	overall oncogenic role	1153:1174	overall oncogenic role of TGF-β/Smad signaling in HCC	1153:1205	Meanwhile, MAPK activation and MAPK-dependent linker phosphorylation of Smad2/3 and their preferential nuclear import are crucial for overall oncogenic role of TGF-β/Smad signaling in HCC.
25934513	8	110	theme	HepG2	1775:1779	arg1	cells					1781:1785	HepG2 cells	1775:1785	HepG2 cells	1775:1785	Also phosphorylation and subcellular distribution of pSmad2/3, Smad4 and Imp7/8 in TGF-β1-stimulated HSC and HepG2 cells were monitored.
25934513	10	111	from	phosphorylation	1939:1953	arg1	HSCs					2084:2087	TGF-β1-stimulated HSCs	2066:2087	TGF-β1-stimulated HSCs	2066:2087	The effect of CASE on domain-specific phosphorylation of Smad2/3 and their subcellular distribution, and the expression of Smad4 and its subcellular distribution in TGF-β1-stimulated HSCs and HepG2 cells were evaluated by using immunofluorescence technique.
25934513	10	111	from	phosphorylation	1939:1953	arg1	cells					2099:2103	HepG2 cells	2093:2103	HepG2 cells	2093:2103	The effect of CASE on domain-specific phosphorylation of Smad2/3 and their subcellular distribution, and the expression of Smad4 and its subcellular distribution in TGF-β1-stimulated HSCs and HepG2 cells were evaluated by using immunofluorescence technique.
25934513	13	112	theme	gene	3021:3024	arg1	expression					3026:3035	PAI-1 gene expression	3015:3035	PAI-1 gene expression	3015:3035	CONCLUSIONS CASE blocked MAPK activation, MAPK-dependent linker phosphorylation of Smad2/3, Smad4 expression, Imp7 expression and their nuclear import leading to significant down-regulation of PAI-1 gene expression; further highlighting the multi-target anti-HCC effect of CASE and its potential drug candidature.
25934513	11	113	theme	pERK	2567:2570	arg1	pp38					2589:2592	pp38	2589:2592	the expression of pERK, pJNK as well as pp38	2549:2592	And the expression of Imp7/8 and their subcellular distribution were assessed by both immunofluorescence and western blot techniques, while PAI-1 gene expression was assessed by western blot RESULTS In vitro, CASE in a concentration-dependent manner increased the expression of pp38 but decreased the expression of pERK and pJNK; however, in vivo, CASE in a dose dependent manner decreased the expression of pERK, pJNK as well as pp38.
25934513	11	113	theme	pERK	2567:2570	arg1	expression					2553:2562	the expression	2549:2562	the expression of pERK, pJNK as well as pp38	2549:2592	And the expression of Imp7/8 and their subcellular distribution were assessed by both immunofluorescence and western blot techniques, while PAI-1 gene expression was assessed by western blot RESULTS In vitro, CASE in a concentration-dependent manner increased the expression of pp38 but decreased the expression of pERK and pJNK; however, in vivo, CASE in a dose dependent manner decreased the expression of pERK, pJNK as well as pp38.
25934513	2	114	used	used	457:460	arg2	formula					515:521	a composite formula	503:521	a composite formula for the treatment of liver related conditions	503:567	Presently, these two herbs are being used either as a single herbal formulation or a composite formula for the treatment of liver related conditions.
25934513	2	114	used	used	457:460	arg2	herbs					441:445	these two herbs	431:445	these two herbs	431:445	Presently, these two herbs are being used either as a single herbal formulation or a composite formula for the treatment of liver related conditions.
25934513	2	114	used	used	457:460	arg2	formulation					488:498	a single herbal formulation	472:498	a single herbal formulation	472:498	Presently, these two herbs are being used either as a single herbal formulation or a composite formula for the treatment of liver related conditions.
25934513	5	115	theme	nuclear	1122:1128	arg1	import					1130:1135	their preferential nuclear import	1103:1135	their preferential nuclear import	1103:1135	Meanwhile, MAPK activation and MAPK-dependent linker phosphorylation of Smad2/3 and their preferential nuclear import are crucial for overall oncogenic role of TGF-β/Smad signaling in HCC.
25934513	13	116	theme	CONCLUSIONS	2822:2832	arg1	CASE					2834:2837	CONCLUSIONS CASE	2822:2837	CONCLUSIONS CASE	2822:2837	CONCLUSIONS CASE blocked MAPK activation, MAPK-dependent linker phosphorylation of Smad2/3, Smad4 expression, Imp7 expression and their nuclear import leading to significant down-regulation of PAI-1 gene expression; further highlighting the multi-target anti-HCC effect of CASE and its potential drug candidature.
25934513	1	117	theme	RELEVANCE	174:182	arg1	Lamiaceae					259:267	Lamiaceae	259:267	Lamiaceae	259:267	ETHNOPHARMACOLOGICAL RELEVANCE Astragalus membranaceus Bunge (Leguminosae) and Salvia miltiorrhiza Bunge (Lamiaceae) are two important Chinese herbs with a long history of extensive ethnobotanical usage in the treatment of liver-related diseases over many centuries.
25934513	1	117	theme	RELEVANCE	174:182	arg1	herbs					296:300	two important Chinese herbs	274:300	two important Chinese herbs with a long history of extensive ethnobotanical usage in the treatment of liver-related diseases over many centuries	274:417	ETHNOPHARMACOLOGICAL RELEVANCE Astragalus membranaceus Bunge (Leguminosae) and Salvia miltiorrhiza Bunge (Lamiaceae) are two important Chinese herbs with a long history of extensive ethnobotanical usage in the treatment of liver-related diseases over many centuries.
25934513	1	117	theme	RELEVANCE	174:182	arg1	membranaceus					195:206	ETHNOPHARMACOLOGICAL RELEVANCE Astragalus membranaceus	153:206	ETHNOPHARMACOLOGICAL RELEVANCE Astragalus membranaceus Bunge (Leguminosae) and Salvia miltiorrhiza Bunge (Lamiaceae)	153:268	ETHNOPHARMACOLOGICAL RELEVANCE Astragalus membranaceus Bunge (Leguminosae) and Salvia miltiorrhiza Bunge (Lamiaceae) are two important Chinese herbs with a long history of extensive ethnobotanical usage in the treatment of liver-related diseases over many centuries.
25934513	1	117	theme	RELEVANCE	174:182	arg1	Leguminosae					215:225	Leguminosae	215:225	Leguminosae	215:225	ETHNOPHARMACOLOGICAL RELEVANCE Astragalus membranaceus Bunge (Leguminosae) and Salvia miltiorrhiza Bunge (Lamiaceae) are two important Chinese herbs with a long history of extensive ethnobotanical usage in the treatment of liver-related diseases over many centuries.
25934513	10	118	theme	Smad4	2024:2028	arg1	distribution					2050:2061	its subcellular distribution	2034:2061	its subcellular distribution	2034:2061	The effect of CASE on domain-specific phosphorylation of Smad2/3 and their subcellular distribution, and the expression of Smad4 and its subcellular distribution in TGF-β1-stimulated HSCs and HepG2 cells were evaluated by using immunofluorescence technique.
25934513	10	118	theme	Smad4	2024:2028	arg1	expression					2010:2019	the expression	2006:2019	the expression of Smad4	2006:2028	The effect of CASE on domain-specific phosphorylation of Smad2/3 and their subcellular distribution, and the expression of Smad4 and its subcellular distribution in TGF-β1-stimulated HSCs and HepG2 cells were evaluated by using immunofluorescence technique.
25934513	10	118	theme	Smad4	2024:2028	arg1	phosphorylation					1939:1953	domain-specific phosphorylation	1923:1953	domain-specific phosphorylation of Smad2/3	1923:1964	The effect of CASE on domain-specific phosphorylation of Smad2/3 and their subcellular distribution, and the expression of Smad4 and its subcellular distribution in TGF-β1-stimulated HSCs and HepG2 cells were evaluated by using immunofluorescence technique.
25934513	10	118	theme	Smad4	2024:2028	arg1	distribution					1988:1999	their subcellular distribution	1970:1999	their subcellular distribution	1970:1999	The effect of CASE on domain-specific phosphorylation of Smad2/3 and their subcellular distribution, and the expression of Smad4 and its subcellular distribution in TGF-β1-stimulated HSCs and HepG2 cells were evaluated by using immunofluorescence technique.
25934513	7	119	from	effect	1479:1484	arg1	activation					1501:1510	the activation	1497:1510	the activation of the MAPKs (pERK, pJNK and pp38) in TGF-β1-stimulated hepatic stellate cells (HSCs), HepG2 cells	1497:1609	MATERIALS AND METHODS We used immunofluorescence and western blot techniques to monitor effect of CASE on the activation of the MAPKs (pERK, pJNK and pp38) in TGF-β1-stimulated hepatic stellate cells (HSCs), HepG2 cells and also diethylnitrosamine (DEN)-induced HCC in rats.
25934513	7	120	theme	HepG2	1599:1603	arg1	cells					1605:1609	HepG2 cells	1599:1609	HepG2 cells	1599:1609	MATERIALS AND METHODS We used immunofluorescence and western blot techniques to monitor effect of CASE on the activation of the MAPKs (pERK, pJNK and pp38) in TGF-β1-stimulated hepatic stellate cells (HSCs), HepG2 cells and also diethylnitrosamine (DEN)-induced HCC in rats.
25934513	7	120	theme	HepG2	1599:1603	arg1	cells					1585:1589	TGF-β1-stimulated hepatic stellate cells	1550:1589	TGF-β1-stimulated hepatic stellate cells (HSCs)	1550:1596	MATERIALS AND METHODS We used immunofluorescence and western blot techniques to monitor effect of CASE on the activation of the MAPKs (pERK, pJNK and pp38) in TGF-β1-stimulated hepatic stellate cells (HSCs), HepG2 cells and also diethylnitrosamine (DEN)-induced HCC in rats.
25934513	11	121	theme	western	2268:2274	arg1	techniques					2281:2290	western blot techniques	2268:2290	western blot techniques	2268:2290	And the expression of Imp7/8 and their subcellular distribution were assessed by both immunofluorescence and western blot techniques, while PAI-1 gene expression was assessed by western blot RESULTS In vitro, CASE in a concentration-dependent manner increased the expression of pp38 but decreased the expression of pERK and pJNK; however, in vivo, CASE in a dose dependent manner decreased the expression of pERK, pJNK as well as pp38.
25934513	6	122	theme	CASE	1255:1258	arg1	effect					1245:1250	the effect	1241:1250	the effect of CASE on the MAPK pathway	1241:1278	To elucidate further, we studied the effect of CASE on the MAPK pathway and how it affects MAPK-dependent regulation of TGF-β/Smad signaling using both cell and animal models of HCC.
25934513	0	123	theme	hepatocellular	101:114	arg1	carcinoma					116:124	hepatocellular carcinoma	101:124	hepatocellular carcinoma	101:124	Compound Astragalus and Salvia miltiorrhiza extracts modulate MAPK-regulated TGF-β/Smad signaling in hepatocellular carcinoma by multi-target mechanism.
25934513	1	124	theme	liver-related	376:388	arg1	diseases					390:397	liver-related diseases	376:397	liver-related diseases over many centuries	376:417	ETHNOPHARMACOLOGICAL RELEVANCE Astragalus membranaceus Bunge (Leguminosae) and Salvia miltiorrhiza Bunge (Lamiaceae) are two important Chinese herbs with a long history of extensive ethnobotanical usage in the treatment of liver-related diseases over many centuries.
25934513	13	125	theme	nuclear	2958:2964	arg1	import					2966:2971	their nuclear import	2952:2971	their nuclear import leading to significant down-regulation of PAI-1 gene expression	2952:3035	CONCLUSIONS CASE blocked MAPK activation, MAPK-dependent linker phosphorylation of Smad2/3, Smad4 expression, Imp7 expression and their nuclear import leading to significant down-regulation of PAI-1 gene expression; further highlighting the multi-target anti-HCC effect of CASE and its potential drug candidature.
25934513	2	126	theme	related	550:556	arg1	conditions					558:567	liver related conditions	544:567	liver related conditions	544:567	Presently, these two herbs are being used either as a single herbal formulation or a composite formula for the treatment of liver related conditions.
25934513	8	127	from	phosphorylation	1671:1685	arg1	HSC					1767:1769	TGF-β1-stimulated HSC	1749:1769	TGF-β1-stimulated HSC	1749:1769	Also phosphorylation and subcellular distribution of pSmad2/3, Smad4 and Imp7/8 in TGF-β1-stimulated HSC and HepG2 cells were monitored.
25934513	8	127	from	phosphorylation	1671:1685	arg1	cells					1781:1785	HepG2 cells	1775:1785	HepG2 cells	1775:1785	Also phosphorylation and subcellular distribution of pSmad2/3, Smad4 and Imp7/8 in TGF-β1-stimulated HSC and HepG2 cells were monitored.
25934513	4	128	theme	Salvia	799:804	arg1	extract					819:825	Salvia miltiorrhiza extract	799:825	Salvia miltiorrhiza extract	799:825	Previously, we have reported that Compound Astragalus and Salvia miltiorrhiza extract (CASE), a synergized composite extract from Astragalus membranaceus and Salvia miltiorrhiza ameliorates liver fibrosis and hepatocellular carcinoma (HCC) by modulating the TGF-β/Smad pathway.
25934513	5	129	theme	MAPK	1030:1033	arg1	activation					1035:1044	MAPK activation	1030:1044	MAPK activation	1030:1044	Meanwhile, MAPK activation and MAPK-dependent linker phosphorylation of Smad2/3 and their preferential nuclear import are crucial for overall oncogenic role of TGF-β/Smad signaling in HCC.
25934513	11	130	theme	blot	2276:2279	arg1	techniques					2281:2290	western blot techniques	2268:2290	western blot techniques	2268:2290	And the expression of Imp7/8 and their subcellular distribution were assessed by both immunofluorescence and western blot techniques, while PAI-1 gene expression was assessed by western blot RESULTS In vitro, CASE in a concentration-dependent manner increased the expression of pp38 but decreased the expression of pERK and pJNK; however, in vivo, CASE in a dose dependent manner decreased the expression of pERK, pJNK as well as pp38.
25934513	5	131	theme	signaling	1190:1198	arg1	role					1171:1174	overall oncogenic role	1153:1174	overall oncogenic role of TGF-β/Smad signaling in HCC	1153:1205	Meanwhile, MAPK activation and MAPK-dependent linker phosphorylation of Smad2/3 and their preferential nuclear import are crucial for overall oncogenic role of TGF-β/Smad signaling in HCC.
25934513	2	132	theme	single	474:479	arg1	herbs					441:445	these two herbs	431:445	these two herbs	431:445	Presently, these two herbs are being used either as a single herbal formulation or a composite formula for the treatment of liver related conditions.
25934513	2	132	theme	single	474:479	arg1	formulation					488:498	a single herbal formulation	472:498	a single herbal formulation	472:498	Presently, these two herbs are being used either as a single herbal formulation or a composite formula for the treatment of liver related conditions.
25934513	0	133	theme	Salvia	24:29	arg1	extracts					44:51	Salvia miltiorrhiza extracts	24:51	Salvia miltiorrhiza extracts	24:51	Compound Astragalus and Salvia miltiorrhiza extracts modulate MAPK-regulated TGF-β/Smad signaling in hepatocellular carcinoma by multi-target mechanism.
25934513	13	134	theme	drug	3118:3121	arg1	candidature					3123:3133	its potential drug candidature	3104:3133	its potential drug candidature	3104:3133	CONCLUSIONS CASE blocked MAPK activation, MAPK-dependent linker phosphorylation of Smad2/3, Smad4 expression, Imp7 expression and their nuclear import leading to significant down-regulation of PAI-1 gene expression; further highlighting the multi-target anti-HCC effect of CASE and its potential drug candidature.
25934513	8	135	from	distribution	1703:1714	arg1	HSC					1767:1769	TGF-β1-stimulated HSC	1749:1769	TGF-β1-stimulated HSC	1749:1769	Also phosphorylation and subcellular distribution of pSmad2/3, Smad4 and Imp7/8 in TGF-β1-stimulated HSC and HepG2 cells were monitored.
25934513	8	135	from	distribution	1703:1714	arg1	cells					1781:1785	HepG2 cells	1775:1785	HepG2 cells	1775:1785	Also phosphorylation and subcellular distribution of pSmad2/3, Smad4 and Imp7/8 in TGF-β1-stimulated HSC and HepG2 cells were monitored.
25934513	1	136	theme	ethnobotanical	335:348	arg1	usage					350:354	extensive ethnobotanical usage	325:354	extensive ethnobotanical usage in the treatment of liver-related diseases over many centuries	325:417	ETHNOPHARMACOLOGICAL RELEVANCE Astragalus membranaceus Bunge (Leguminosae) and Salvia miltiorrhiza Bunge (Lamiaceae) are two important Chinese herbs with a long history of extensive ethnobotanical usage in the treatment of liver-related diseases over many centuries.
25934513	12	137	from	pSmad3C	2722:2728	arg1	cells					2739:2743	HepG2 cells	2733:2743	HepG2 cells	2733:2743	Also, CASE concentration dependently inhibited pSmad2C/L, pSmad3L, Smad4, Imp7/8 and their nuclear import; it had no effect on pSmad3C in HepG2 cells; significantly decreased PAI-1 gene expression in both in vitro and in vivo.
25934513	4	138	theme	synergized	837:846	arg1	extract					858:864	a synergized composite extract	835:864	a synergized composite extract from Astragalus membranaceus	835:893	Previously, we have reported that Compound Astragalus and Salvia miltiorrhiza extract (CASE), a synergized composite extract from Astragalus membranaceus and Salvia miltiorrhiza ameliorates liver fibrosis and hepatocellular carcinoma (HCC) by modulating the TGF-β/Smad pathway.
25934513	0	139	theme	MAPK-regulated	62:75	arg1	signaling					88:96	MAPK-regulated TGF-β/Smad signaling	62:96	MAPK-regulated TGF-β/Smad signaling in hepatocellular carcinoma	62:124	Compound Astragalus and Salvia miltiorrhiza extracts modulate MAPK-regulated TGF-β/Smad signaling in hepatocellular carcinoma by multi-target mechanism.
25934513	7	140	theme	hepatic	1568:1574	arg1	cells					1605:1609	HepG2 cells	1599:1609	HepG2 cells	1599:1609	MATERIALS AND METHODS We used immunofluorescence and western blot techniques to monitor effect of CASE on the activation of the MAPKs (pERK, pJNK and pp38) in TGF-β1-stimulated hepatic stellate cells (HSCs), HepG2 cells and also diethylnitrosamine (DEN)-induced HCC in rats.
25934513	7	140	theme	hepatic	1568:1574	arg1	HSCs					1592:1595	HSCs	1592:1595	HSCs	1592:1595	MATERIALS AND METHODS We used immunofluorescence and western blot techniques to monitor effect of CASE on the activation of the MAPKs (pERK, pJNK and pp38) in TGF-β1-stimulated hepatic stellate cells (HSCs), HepG2 cells and also diethylnitrosamine (DEN)-induced HCC in rats.
25934513	7	140	theme	hepatic	1568:1574	arg1	cells					1585:1589	TGF-β1-stimulated hepatic stellate cells	1550:1589	TGF-β1-stimulated hepatic stellate cells (HSCs)	1550:1596	MATERIALS AND METHODS We used immunofluorescence and western blot techniques to monitor effect of CASE on the activation of the MAPKs (pERK, pJNK and pp38) in TGF-β1-stimulated hepatic stellate cells (HSCs), HepG2 cells and also diethylnitrosamine (DEN)-induced HCC in rats.
25934513	5	141	theme	TGF-β/Smad	1179:1188	arg1	signaling					1190:1198	TGF-β/Smad signaling	1179:1198	TGF-β/Smad signaling	1179:1198	Meanwhile, MAPK activation and MAPK-dependent linker phosphorylation of Smad2/3 and their preferential nuclear import are crucial for overall oncogenic role of TGF-β/Smad signaling in HCC.
25934513	13	142	theme	CASE	3095:3098	arg1	effect					3085:3090	the multi-target anti-HCC effect	3059:3090	the multi-target anti-HCC effect of CASE and its potential drug candidature	3059:3133	CONCLUSIONS CASE blocked MAPK activation, MAPK-dependent linker phosphorylation of Smad2/3, Smad4 expression, Imp7 expression and their nuclear import leading to significant down-regulation of PAI-1 gene expression; further highlighting the multi-target anti-HCC effect of CASE and its potential drug candidature.
25934513	9	143	theme	gene	1848:1851	arg1	expression					1807:1816	The expression	1803:1816	The expression of pERK, pJNK, pp38 and PAI-1 gene	1803:1851	The expression of pERK, pJNK, pp38 and PAI-1 gene were monitored by using western blot technique.
25934513	10	144	from	distribution	1988:1999	arg1	HSCs					2084:2087	TGF-β1-stimulated HSCs	2066:2087	TGF-β1-stimulated HSCs	2066:2087	The effect of CASE on domain-specific phosphorylation of Smad2/3 and their subcellular distribution, and the expression of Smad4 and its subcellular distribution in TGF-β1-stimulated HSCs and HepG2 cells were evaluated by using immunofluorescence technique.
25934513	10	144	from	distribution	1988:1999	arg1	cells					2099:2103	HepG2 cells	2093:2103	HepG2 cells	2093:2103	The effect of CASE on domain-specific phosphorylation of Smad2/3 and their subcellular distribution, and the expression of Smad4 and its subcellular distribution in TGF-β1-stimulated HSCs and HepG2 cells were evaluated by using immunofluorescence technique.
25934513	11	145	theme	dependent	2522:2530	arg1	manner					2532:2537	a dose dependent manner	2515:2537	a dose dependent manner	2515:2537	And the expression of Imp7/8 and their subcellular distribution were assessed by both immunofluorescence and western blot techniques, while PAI-1 gene expression was assessed by western blot RESULTS In vitro, CASE in a concentration-dependent manner increased the expression of pp38 but decreased the expression of pERK and pJNK; however, in vivo, CASE in a dose dependent manner decreased the expression of pERK, pJNK as well as pp38.
25934513	7	146	dep	MAPKs	1519:1523	arg1	pERK					1526:1529	pERK	1526:1529	pERK	1526:1529	MATERIALS AND METHODS We used immunofluorescence and western blot techniques to monitor effect of CASE on the activation of the MAPKs (pERK, pJNK and pp38) in TGF-β1-stimulated hepatic stellate cells (HSCs), HepG2 cells and also diethylnitrosamine (DEN)-induced HCC in rats.
25934513	7	146	dep	MAPKs	1519:1523	arg1	MAPKs					1519:1523	the MAPKs	1515:1523	the MAPKs (pERK, pJNK and pp38)	1515:1545	MATERIALS AND METHODS We used immunofluorescence and western blot techniques to monitor effect of CASE on the activation of the MAPKs (pERK, pJNK and pp38) in TGF-β1-stimulated hepatic stellate cells (HSCs), HepG2 cells and also diethylnitrosamine (DEN)-induced HCC in rats.
25934513	7	146	dep	MAPKs	1519:1523	arg1	pp38					1541:1544	pp38	1541:1544	pp38	1541:1544	MATERIALS AND METHODS We used immunofluorescence and western blot techniques to monitor effect of CASE on the activation of the MAPKs (pERK, pJNK and pp38) in TGF-β1-stimulated hepatic stellate cells (HSCs), HepG2 cells and also diethylnitrosamine (DEN)-induced HCC in rats.
25934513	7	146	dep	MAPKs	1519:1523	arg1	pJNK					1532:1535	pJNK	1532:1535	pJNK	1532:1535	MATERIALS AND METHODS We used immunofluorescence and western blot techniques to monitor effect of CASE on the activation of the MAPKs (pERK, pJNK and pp38) in TGF-β1-stimulated hepatic stellate cells (HSCs), HepG2 cells and also diethylnitrosamine (DEN)-induced HCC in rats.
25934513	13	147	theme	Smad2/3	2905:2911	arg1	expression					2937:2946	Imp7 expression	2932:2946	Imp7 expression	2932:2946	CONCLUSIONS CASE blocked MAPK activation, MAPK-dependent linker phosphorylation of Smad2/3, Smad4 expression, Imp7 expression and their nuclear import leading to significant down-regulation of PAI-1 gene expression; further highlighting the multi-target anti-HCC effect of CASE and its potential drug candidature.
25934513	13	147	theme	Smad2/3	2905:2911	arg1	activation					2852:2861	MAPK activation	2847:2861	MAPK activation	2847:2861	CONCLUSIONS CASE blocked MAPK activation, MAPK-dependent linker phosphorylation of Smad2/3, Smad4 expression, Imp7 expression and their nuclear import leading to significant down-regulation of PAI-1 gene expression; further highlighting the multi-target anti-HCC effect of CASE and its potential drug candidature.
25934513	13	147	theme	Smad2/3	2905:2911	arg1	import					2966:2971	their nuclear import	2952:2971	their nuclear import leading to significant down-regulation of PAI-1 gene expression	2952:3035	CONCLUSIONS CASE blocked MAPK activation, MAPK-dependent linker phosphorylation of Smad2/3, Smad4 expression, Imp7 expression and their nuclear import leading to significant down-regulation of PAI-1 gene expression; further highlighting the multi-target anti-HCC effect of CASE and its potential drug candidature.
25934513	13	147	theme	Smad2/3	2905:2911	arg1	expression					2920:2929	Smad4 expression	2914:2929	Smad4 expression	2914:2929	CONCLUSIONS CASE blocked MAPK activation, MAPK-dependent linker phosphorylation of Smad2/3, Smad4 expression, Imp7 expression and their nuclear import leading to significant down-regulation of PAI-1 gene expression; further highlighting the multi-target anti-HCC effect of CASE and its potential drug candidature.
25934513	13	147	theme	Smad2/3	2905:2911	arg1	phosphorylation					2886:2900	MAPK-dependent linker phosphorylation	2864:2900	MAPK-dependent linker phosphorylation of Smad2/3	2864:2911	CONCLUSIONS CASE blocked MAPK activation, MAPK-dependent linker phosphorylation of Smad2/3, Smad4 expression, Imp7 expression and their nuclear import leading to significant down-regulation of PAI-1 gene expression; further highlighting the multi-target anti-HCC effect of CASE and its potential drug candidature.
25934513	13	148	theme	anti-HCC	3076:3083	arg1	effect					3085:3090	the multi-target anti-HCC effect	3059:3090	the multi-target anti-HCC effect of CASE and its potential drug candidature	3059:3133	CONCLUSIONS CASE blocked MAPK activation, MAPK-dependent linker phosphorylation of Smad2/3, Smad4 expression, Imp7 expression and their nuclear import leading to significant down-regulation of PAI-1 gene expression; further highlighting the multi-target anti-HCC effect of CASE and its potential drug candidature.
25934513	9	149	theme	pp38	1833:1836	arg1	expression					1807:1816	The expression	1803:1816	The expression of pERK, pJNK, pp38 and PAI-1 gene	1803:1851	The expression of pERK, pJNK, pp38 and PAI-1 gene were monitored by using western blot technique.
25934513	13	150	theme	Smad4	2914:2918	arg1	expression					2920:2929	Smad4 expression	2914:2929	Smad4 expression	2914:2929	CONCLUSIONS CASE blocked MAPK activation, MAPK-dependent linker phosphorylation of Smad2/3, Smad4 expression, Imp7 expression and their nuclear import leading to significant down-regulation of PAI-1 gene expression; further highlighting the multi-target anti-HCC effect of CASE and its potential drug candidature.
25934513	5	151	theme	linker	1065:1070	arg1	phosphorylation					1072:1086	MAPK-dependent linker phosphorylation	1050:1086	MAPK-dependent linker phosphorylation of Smad2/3	1050:1097	Meanwhile, MAPK activation and MAPK-dependent linker phosphorylation of Smad2/3 and their preferential nuclear import are crucial for overall oncogenic role of TGF-β/Smad signaling in HCC.
25934513	13	152	theme	multi-target	3063:3074	arg1	effect					3085:3090	the multi-target anti-HCC effect	3059:3090	the multi-target anti-HCC effect of CASE and its potential drug candidature	3059:3133	CONCLUSIONS CASE blocked MAPK activation, MAPK-dependent linker phosphorylation of Smad2/3, Smad4 expression, Imp7 expression and their nuclear import leading to significant down-regulation of PAI-1 gene expression; further highlighting the multi-target anti-HCC effect of CASE and its potential drug candidature.
25934513	10	153	theme	TGF-β1-stimulated	2066:2082	arg1	HSCs					2084:2087	TGF-β1-stimulated HSCs	2066:2087	TGF-β1-stimulated HSCs	2066:2087	The effect of CASE on domain-specific phosphorylation of Smad2/3 and their subcellular distribution, and the expression of Smad4 and its subcellular distribution in TGF-β1-stimulated HSCs and HepG2 cells were evaluated by using immunofluorescence technique.
25934513	10	154	theme	CASE	1915:1918	arg1	effect					1905:1910	The effect	1901:1910	The effect of CASE on domain-specific phosphorylation of Smad2/3 and their subcellular distribution, and the expression of Smad4 and its subcellular distribution in TGF-β1-stimulated HSCs and HepG2 cells	1901:2103	The effect of CASE on domain-specific phosphorylation of Smad2/3 and their subcellular distribution, and the expression of Smad4 and its subcellular distribution in TGF-β1-stimulated HSCs and HepG2 cells were evaluated by using immunofluorescence technique.
25934513	9	155	theme	pERK	1821:1824	arg1	expression					1807:1816	The expression	1803:1816	The expression of pERK, pJNK, pp38 and PAI-1 gene	1803:1851	The expression of pERK, pJNK, pp38 and PAI-1 gene were monitored by using western blot technique.
25934513	1	156	theme	Chinese	288:294	arg1	membranaceus					195:206	ETHNOPHARMACOLOGICAL RELEVANCE Astragalus membranaceus	153:206	ETHNOPHARMACOLOGICAL RELEVANCE Astragalus membranaceus Bunge (Leguminosae) and Salvia miltiorrhiza Bunge (Lamiaceae)	153:268	ETHNOPHARMACOLOGICAL RELEVANCE Astragalus membranaceus Bunge (Leguminosae) and Salvia miltiorrhiza Bunge (Lamiaceae) are two important Chinese herbs with a long history of extensive ethnobotanical usage in the treatment of liver-related diseases over many centuries.
25934513	1	156	theme	Chinese	288:294	arg1	herbs					296:300	two important Chinese herbs	274:300	two important Chinese herbs with a long history of extensive ethnobotanical usage in the treatment of liver-related diseases over many centuries	274:417	ETHNOPHARMACOLOGICAL RELEVANCE Astragalus membranaceus Bunge (Leguminosae) and Salvia miltiorrhiza Bunge (Lamiaceae) are two important Chinese herbs with a long history of extensive ethnobotanical usage in the treatment of liver-related diseases over many centuries.
25416597	2	0	used	used	270:273	arg2	antibodies					216:225	Monoclonal antibodies	205:225	Monoclonal antibodies specific to cell wall polysaccharides	205:263	Monoclonal antibodies specific to cell wall polysaccharides were used to determine polysaccharide dynamics during the development of both wheat and rice grain.
25416597	4	1	theme	mixed-linkage	610:622	arg1	MLG					632:634	MLG	632:634	MLG	632:634	Arabinoxylan (AX) and mixed-linkage glucan (MLG) have analogous cellular locations in both species, with deposition of AX and MLG coinciding with the start of grain filling.
25416597	4	1	theme	mixed-linkage	610:622	arg1	glucan					624:629	mixed-linkage glucan	610:629	mixed-linkage glucan (MLG)	610:635	Arabinoxylan (AX) and mixed-linkage glucan (MLG) have analogous cellular locations in both species, with deposition of AX and MLG coinciding with the start of grain filling.
25416597	10	2	theme	wheat	1798:1802	arg1	grain					1804:1808	wheat grain	1798:1808	wheat grain	1798:1808	In contrast, the LM6 arabinan epitope was detected in both species around 8 DAA and was transient in wheat grain, but persisted in rice until maturity.
25416597	7	3	theme	Pectic	1088:1093	arg1	HG					1113:1114	HG	1113:1114	HG	1113:1114	Pectic homogalacturonan (HG) was abundant in cell walls of maternal tissues of wheat and rice grain, but only detected in endosperm cell walls of rice in an unesterified HG form.
25416597	7	3	theme	Pectic	1088:1093	arg1	homogalacturonan					1095:1110	Pectic homogalacturonan	1088:1110	Pectic homogalacturonan (HG)	1088:1115	Pectic homogalacturonan (HG) was abundant in cell walls of maternal tissues of wheat and rice grain, but only detected in endosperm cell walls of rice in an unesterified HG form.
25416597	3	4	theme	developmental	565:577	arg1	changes					579:585	developmental changes	565:585	developmental changes	565:585	Wheat and rice grain present near synchronous developmental processes and significantly different endosperm cell wall compositions, allowing the localisation of these polysaccharides to be related to developmental changes.
25416597	9	5	theme	earliest	1562:1569	arg1	stages					1571:1576	the earliest stages	1558:1576	the earliest stages of development in rice endosperm cell walls	1558:1620	Detection of the LM5 galactan epitope showed a clear distinction between wheat and rice, being detected at the earliest stages of development in rice endosperm cell walls, but not detected in wheat endosperm cell walls, only in maternal tissues.
25416597	6	6	theme	extensions	1037:1046	arg1	xyloglucan					974:983	xyloglucan	974:983	xyloglucan	974:983	Callose has been reported to be associated with the formation of cell wall outgrowths during endosperm cellularisation and xyloglucan is here shown to be a component of these anticlinal extensions, occurring transiently in both species.
25416597	6	6	theme	extensions	1037:1046	arg1	component					1007:1015	a component	1005:1015	a component of these anticlinal extensions	1005:1046	Callose has been reported to be associated with the formation of cell wall outgrowths during endosperm cellularisation and xyloglucan is here shown to be a component of these anticlinal extensions, occurring transiently in both species.
25416597	5	7	theme	cell	839:842	arg1	walls					844:848	wheat endosperm cell walls	823:848	wheat endosperm cell walls	823:848	A glucuronoxylan (GUX) epitope was detected in rice, but not wheat endosperm cell walls.
25416597	1	8	theme	important	170:178	arg1	polysaccharides					119:133	Cell wall polysaccharides	109:133	Cell wall polysaccharides of wheat and rice endosperm	109:161	Cell wall polysaccharides of wheat and rice endosperm are an important source of dietary fibre.
25416597	1	8	theme	important	170:178	arg1	source					180:185	an important source	167:185	an important source of dietary fibre	167:202	Cell wall polysaccharides of wheat and rice endosperm are an important source of dietary fibre.
25416597	9	9	from	development	1581:1591	arg1	walls					1616:1620	rice endosperm cell walls	1596:1620	rice endosperm cell walls	1596:1620	Detection of the LM5 galactan epitope showed a clear distinction between wheat and rice, being detected at the earliest stages of development in rice endosperm cell walls, but not detected in wheat endosperm cell walls, only in maternal tissues.
25416597	4	10	theme	analogous	642:650	arg1	locations					661:669	analogous cellular locations	642:669	analogous cellular locations	642:669	Arabinoxylan (AX) and mixed-linkage glucan (MLG) have analogous cellular locations in both species, with deposition of AX and MLG coinciding with the start of grain filling.
25416597	2	11	theme	polysaccharide	288:301	arg1	dynamics					303:310	polysaccharide dynamics	288:310	polysaccharide dynamics	288:310	Monoclonal antibodies specific to cell wall polysaccharides were used to determine polysaccharide dynamics during the development of both wheat and rice grain.
25416597	10	12	from	transient	1785:1793	arg1	grain					1804:1808	wheat grain	1798:1808	wheat grain	1798:1808	In contrast, the LM6 arabinan epitope was detected in both species around 8 DAA and was transient in wheat grain, but persisted in rice until maturity.
25416597	6	13	theme	wall	921:924	arg1	outgrowths					926:935	cell wall outgrowths	916:935	cell wall outgrowths	916:935	Callose has been reported to be associated with the formation of cell wall outgrowths during endosperm cellularisation and xyloglucan is here shown to be a component of these anticlinal extensions, occurring transiently in both species.
25416597	9	14	theme	endosperm	1601:1609	arg1	walls					1616:1620	rice endosperm cell walls	1596:1620	rice endosperm cell walls	1596:1620	Detection of the LM5 galactan epitope showed a clear distinction between wheat and rice, being detected at the earliest stages of development in rice endosperm cell walls, but not detected in wheat endosperm cell walls, only in maternal tissues.
25416597	5	15	theme	wheat	823:827	arg1	walls					844:848	wheat endosperm cell walls	823:848	wheat endosperm cell walls	823:848	A glucuronoxylan (GUX) epitope was detected in rice, but not wheat endosperm cell walls.
25416597	8	16	theme	endosperm	1383:1391	arg1	walls					1398:1402	endosperm cell walls	1383:1402	endosperm cell walls from 12 DAA in rice and 20 DAA in wheat grain	1383:1448	A rhamnogalacturonan-I (RG-I) backbone epitope was observed to be temporally regulated in both species, detected in endosperm cell walls from 12 DAA in rice and 20 DAA in wheat grain.
25416597	4	17	theme	MLG	714:716	arg1	deposition					693:702	deposition	693:702	deposition of AX and MLG coinciding with the start of grain filling	693:759	Arabinoxylan (AX) and mixed-linkage glucan (MLG) have analogous cellular locations in both species, with deposition of AX and MLG coinciding with the start of grain filling.
25416597	1	18	theme	Cell	109:112	arg1	polysaccharides					119:133	Cell wall polysaccharides	109:133	Cell wall polysaccharides of wheat and rice endosperm	109:161	Cell wall polysaccharides of wheat and rice endosperm are an important source of dietary fibre.
25416597	1	18	theme	Cell	109:112	arg1	source					180:185	an important source	167:185	an important source of dietary fibre	167:202	Cell wall polysaccharides of wheat and rice endosperm are an important source of dietary fibre.
25416597	8	19	located	detected	1371:1378	arg2	species					1362:1368	both species	1357:1368	both species	1357:1368	A rhamnogalacturonan-I (RG-I) backbone epitope was observed to be temporally regulated in both species, detected in endosperm cell walls from 12 DAA in rice and 20 DAA in wheat grain.
25416597	8	19	located	detected	1371:1378	arg1	walls					1398:1402	endosperm cell walls	1383:1402	endosperm cell walls from 12 DAA in rice and 20 DAA in wheat grain	1383:1448	A rhamnogalacturonan-I (RG-I) backbone epitope was observed to be temporally regulated in both species, detected in endosperm cell walls from 12 DAA in rice and 20 DAA in wheat grain.
25416597	4	20	theme	grain	747:751	arg1	filling					753:759	grain filling	747:759	grain filling	747:759	Arabinoxylan (AX) and mixed-linkage glucan (MLG) have analogous cellular locations in both species, with deposition of AX and MLG coinciding with the start of grain filling.
25416597	2	21	theme	wall	244:247	arg1	polysaccharides					249:263	cell wall polysaccharides	239:263	cell wall polysaccharides	239:263	Monoclonal antibodies specific to cell wall polysaccharides were used to determine polysaccharide dynamics during the development of both wheat and rice grain.
25416597	0	22	theme	polysaccharide	49:62	arg1	dynamics					64:71	cell wall matrix polysaccharide dynamics	32:71	cell wall matrix polysaccharide dynamics	32:71	Comparative in situ analyses of cell wall matrix polysaccharide dynamics in developing rice and wheat grain.
25416597	1	23	theme	wheat	138:142	arg1	polysaccharides					119:133	Cell wall polysaccharides	109:133	Cell wall polysaccharides of wheat and rice endosperm	109:161	Cell wall polysaccharides of wheat and rice endosperm are an important source of dietary fibre.
25416597	1	23	theme	wheat	138:142	arg1	source					180:185	an important source	167:185	an important source of dietary fibre	167:202	Cell wall polysaccharides of wheat and rice endosperm are an important source of dietary fibre.
25416597	9	24	theme	wheat	1643:1647	arg1	walls					1664:1668	wheat endosperm cell walls	1643:1668	wheat endosperm cell walls	1643:1668	Detection of the LM5 galactan epitope showed a clear distinction between wheat and rice, being detected at the earliest stages of development in rice endosperm cell walls, but not detected in wheat endosperm cell walls, only in maternal tissues.
25416597	3	25	dep	Wheat	365:369	arg1	grain					380:384	grain	380:384	grain	380:384	Wheat and rice grain present near synchronous developmental processes and significantly different endosperm cell wall compositions, allowing the localisation of these polysaccharides to be related to developmental changes.
25416597	4	26	theme	AX	707:708	arg1	deposition					693:702	deposition	693:702	deposition of AX and MLG coinciding with the start of grain filling	693:759	Arabinoxylan (AX) and mixed-linkage glucan (MLG) have analogous cellular locations in both species, with deposition of AX and MLG coinciding with the start of grain filling.
25416597	5	27	theme	glucuronoxylan	764:777	arg1	epitope					785:791	A glucuronoxylan (GUX) epitope	762:791	A glucuronoxylan (GUX) epitope	762:791	A glucuronoxylan (GUX) epitope was detected in rice, but not wheat endosperm cell walls.
25416597	10	28	from	grain	1804:1808	arg1	epitope					1727:1733	the LM6 arabinan epitope	1710:1733	the LM6 arabinan epitope	1710:1733	In contrast, the LM6 arabinan epitope was detected in both species around 8 DAA and was transient in wheat grain, but persisted in rice until maturity.
25416597	10	28	from	grain	1804:1808	arg1	transient					1785:1793	transient	1785:1793	transient	1785:1793	In contrast, the LM6 arabinan epitope was detected in both species around 8 DAA and was transient in wheat grain, but persisted in rice until maturity.
25416597	5	29	located	detected	797:804	arg2	epitope					785:791	A glucuronoxylan (GUX) epitope	762:791	A glucuronoxylan (GUX) epitope	762:791	A glucuronoxylan (GUX) epitope was detected in rice, but not wheat endosperm cell walls.
25416597	5	29	located	detected	797:804	arg1	walls					844:848	wheat endosperm cell walls	823:848	wheat endosperm cell walls	823:848	A glucuronoxylan (GUX) epitope was detected in rice, but not wheat endosperm cell walls.
25416597	5	29	located	detected	797:804	arg1	rice					809:812	rice	809:812	rice	809:812	A glucuronoxylan (GUX) epitope was detected in rice, but not wheat endosperm cell walls.
25416597	1	30	theme	rice	148:151	arg1	polysaccharides					119:133	Cell wall polysaccharides	109:133	Cell wall polysaccharides of wheat and rice endosperm	109:161	Cell wall polysaccharides of wheat and rice endosperm are an important source of dietary fibre.
25416597	1	30	theme	rice	148:151	arg1	source					180:185	an important source	167:185	an important source of dietary fibre	167:202	Cell wall polysaccharides of wheat and rice endosperm are an important source of dietary fibre.
25416597	9	31	theme	cell	1659:1662	arg1	walls					1664:1668	wheat endosperm cell walls	1643:1668	wheat endosperm cell walls	1643:1668	Detection of the LM5 galactan epitope showed a clear distinction between wheat and rice, being detected at the earliest stages of development in rice endosperm cell walls, but not detected in wheat endosperm cell walls, only in maternal tissues.
25416597	7	32	from	abundant	1121:1128	arg1	walls					1138:1142	cell walls	1133:1142	cell walls of maternal tissues of wheat and rice grain	1133:1186	Pectic homogalacturonan (HG) was abundant in cell walls of maternal tissues of wheat and rice grain, but only detected in endosperm cell walls of rice in an unesterified HG form.
25416597	7	33	theme	wheat	1167:1171	arg1	tissues					1156:1162	maternal tissues	1147:1162	maternal tissues of wheat and rice grain	1147:1186	Pectic homogalacturonan (HG) was abundant in cell walls of maternal tissues of wheat and rice grain, but only detected in endosperm cell walls of rice in an unesterified HG form.
25416597	7	34	theme	tissues	1156:1162	arg1	walls					1138:1142	cell walls	1133:1142	cell walls of maternal tissues of wheat and rice grain	1133:1186	Pectic homogalacturonan (HG) was abundant in cell walls of maternal tissues of wheat and rice grain, but only detected in endosperm cell walls of rice in an unesterified HG form.
25416597	0	35	dep	rice	87:90	arg1	grain					102:106	grain	102:106	grain	102:106	Comparative in situ analyses of cell wall matrix polysaccharide dynamics in developing rice and wheat grain.
25416597	8	36	theme	wheat	1438:1442	arg1	grain					1444:1448	wheat grain	1438:1448	wheat grain	1438:1448	A rhamnogalacturonan-I (RG-I) backbone epitope was observed to be temporally regulated in both species, detected in endosperm cell walls from 12 DAA in rice and 20 DAA in wheat grain.
25416597	0	37	theme	dynamics	64:71	arg1	analyses					20:27	Comparative in situ analyses	0:27	Comparative in situ analyses of cell wall matrix polysaccharide dynamics in developing rice and wheat grain.	0:107	Comparative in situ analyses of cell wall matrix polysaccharide dynamics in developing rice and wheat grain.
25416597	0	38	theme	Comparative	0:10	arg1	analyses					20:27	Comparative in situ analyses	0:27	Comparative in situ analyses of cell wall matrix polysaccharide dynamics in developing rice and wheat grain.	0:107	Comparative in situ analyses of cell wall matrix polysaccharide dynamics in developing rice and wheat grain.
25416597	9	39	theme	galactan	1472:1479	arg1	epitope					1481:1487	the LM5 galactan epitope	1464:1487	the LM5 galactan epitope	1464:1487	Detection of the LM5 galactan epitope showed a clear distinction between wheat and rice, being detected at the earliest stages of development in rice endosperm cell walls, but not detected in wheat endosperm cell walls, only in maternal tissues.
25416597	6	40	theme	anticlinal	1026:1035	arg1	extensions					1037:1046	these anticlinal extensions	1020:1046	these anticlinal extensions	1020:1046	Callose has been reported to be associated with the formation of cell wall outgrowths during endosperm cellularisation and xyloglucan is here shown to be a component of these anticlinal extensions, occurring transiently in both species.
25416597	2	41	dep	wheat	343:347	arg1	grain					358:362	grain	358:362	grain	358:362	Monoclonal antibodies specific to cell wall polysaccharides were used to determine polysaccharide dynamics during the development of both wheat and rice grain.
25416597	3	42	theme	wall	478:481	arg1	compositions					483:494	significantly different endosperm cell wall compositions	439:494	significantly different endosperm cell wall compositions	439:494	Wheat and rice grain present near synchronous developmental processes and significantly different endosperm cell wall compositions, allowing the localisation of these polysaccharides to be related to developmental changes.
25416597	7	43	theme	cell	1133:1136	arg1	walls					1138:1142	cell walls	1133:1142	cell walls of maternal tissues of wheat and rice grain	1133:1186	Pectic homogalacturonan (HG) was abundant in cell walls of maternal tissues of wheat and rice grain, but only detected in endosperm cell walls of rice in an unesterified HG form.
25416597	7	44	theme	unesterified	1245:1256	arg1	form					1261:1264	an unesterified HG form	1242:1264	an unesterified HG form	1242:1264	Pectic homogalacturonan (HG) was abundant in cell walls of maternal tissues of wheat and rice grain, but only detected in endosperm cell walls of rice in an unesterified HG form.
25416597	3	45	theme	synchronous	399:409	arg1	processes					425:433	synchronous developmental processes	399:433	synchronous developmental processes	399:433	Wheat and rice grain present near synchronous developmental processes and significantly different endosperm cell wall compositions, allowing the localisation of these polysaccharides to be related to developmental changes.
25416597	3	46	theme	endosperm	463:471	arg1	compositions					483:494	significantly different endosperm cell wall compositions	439:494	significantly different endosperm cell wall compositions	439:494	Wheat and rice grain present near synchronous developmental processes and significantly different endosperm cell wall compositions, allowing the localisation of these polysaccharides to be related to developmental changes.
25416597	5	47	theme	GUX	780:782	arg1	epitope					785:791	A glucuronoxylan (GUX) epitope	762:791	A glucuronoxylan (GUX) epitope	762:791	A glucuronoxylan (GUX) epitope was detected in rice, but not wheat endosperm cell walls.
25416597	9	48	theme	clear	1498:1502	arg1	distinction					1504:1514	a clear distinction	1496:1514	a clear distinction between wheat and rice	1496:1537	Detection of the LM5 galactan epitope showed a clear distinction between wheat and rice, being detected at the earliest stages of development in rice endosperm cell walls, but not detected in wheat endosperm cell walls, only in maternal tissues.
25416597	10	49	theme	LM6	1714:1716	arg1	epitope					1727:1733	the LM6 arabinan epitope	1710:1733	the LM6 arabinan epitope	1710:1733	In contrast, the LM6 arabinan epitope was detected in both species around 8 DAA and was transient in wheat grain, but persisted in rice until maturity.
25416597	10	49	theme	LM6	1714:1716	arg1	transient					1785:1793	transient	1785:1793	transient	1785:1793	In contrast, the LM6 arabinan epitope was detected in both species around 8 DAA and was transient in wheat grain, but persisted in rice until maturity.
25416597	2	50	theme	wheat	343:347	arg1	development					323:333	the development	319:333	the development of both wheat and rice grain	319:362	Monoclonal antibodies specific to cell wall polysaccharides were used to determine polysaccharide dynamics during the development of both wheat and rice grain.
25416597	0	51	theme	wall	37:40	arg1	dynamics					64:71	cell wall matrix polysaccharide dynamics	32:71	cell wall matrix polysaccharide dynamics	32:71	Comparative in situ analyses of cell wall matrix polysaccharide dynamics in developing rice and wheat grain.
25416597	10	52	theme	arabinan	1718:1725	arg1	epitope					1727:1733	the LM6 arabinan epitope	1710:1733	the LM6 arabinan epitope	1710:1733	In contrast, the LM6 arabinan epitope was detected in both species around 8 DAA and was transient in wheat grain, but persisted in rice until maturity.
25416597	10	52	theme	arabinan	1718:1725	arg1	transient					1785:1793	transient	1785:1793	transient	1785:1793	In contrast, the LM6 arabinan epitope was detected in both species around 8 DAA and was transient in wheat grain, but persisted in rice until maturity.
25416597	7	53	theme	cell	1220:1223	arg1	walls					1225:1229	endosperm cell walls	1210:1229	endosperm cell walls of rice	1210:1237	Pectic homogalacturonan (HG) was abundant in cell walls of maternal tissues of wheat and rice grain, but only detected in endosperm cell walls of rice in an unesterified HG form.
25416597	3	54	theme	polysaccharides	532:546	arg1	localisation					510:521	the localisation	506:521	the localisation of these polysaccharides	506:546	Wheat and rice grain present near synchronous developmental processes and significantly different endosperm cell wall compositions, allowing the localisation of these polysaccharides to be related to developmental changes.
25416597	3	54	theme	polysaccharides	532:546	arg1	related					554:560	related	554:560	related	554:560	Wheat and rice grain present near synchronous developmental processes and significantly different endosperm cell wall compositions, allowing the localisation of these polysaccharides to be related to developmental changes.
25416597	2	55	theme	rice	353:356	arg1	development					323:333	the development	319:333	the development of both wheat and rice grain	319:362	Monoclonal antibodies specific to cell wall polysaccharides were used to determine polysaccharide dynamics during the development of both wheat and rice grain.
25416597	5	56	theme	endosperm	829:837	arg1	walls					844:848	wheat endosperm cell walls	823:848	wheat endosperm cell walls	823:848	A glucuronoxylan (GUX) epitope was detected in rice, but not wheat endosperm cell walls.
25416597	8	57	from	DAA	1431:1433	arg1	grain					1444:1448	wheat grain	1438:1448	wheat grain	1438:1448	A rhamnogalacturonan-I (RG-I) backbone epitope was observed to be temporally regulated in both species, detected in endosperm cell walls from 12 DAA in rice and 20 DAA in wheat grain.
25416597	7	58	theme	rice	1177:1180	arg1	grain					1182:1186	rice grain	1177:1186	rice grain	1177:1186	Pectic homogalacturonan (HG) was abundant in cell walls of maternal tissues of wheat and rice grain, but only detected in endosperm cell walls of rice in an unesterified HG form.
25416597	6	59	theme	endosperm	944:952	arg1	cellularisation					954:968	endosperm cellularisation	944:968	endosperm cellularisation	944:968	Callose has been reported to be associated with the formation of cell wall outgrowths during endosperm cellularisation and xyloglucan is here shown to be a component of these anticlinal extensions, occurring transiently in both species.
25416597	9	60	theme	development	1581:1591	arg1	stages					1571:1576	the earliest stages	1558:1576	the earliest stages of development in rice endosperm cell walls	1558:1620	Detection of the LM5 galactan epitope showed a clear distinction between wheat and rice, being detected at the earliest stages of development in rice endosperm cell walls, but not detected in wheat endosperm cell walls, only in maternal tissues.
25416597	6	61	theme	outgrowths	926:935	arg1	formation					903:911	the formation	899:911	the formation of cell wall outgrowths during endosperm cellularisation	899:968	Callose has been reported to be associated with the formation of cell wall outgrowths during endosperm cellularisation and xyloglucan is here shown to be a component of these anticlinal extensions, occurring transiently in both species.
25416597	9	62	theme	rice	1596:1599	arg1	walls					1616:1620	rice endosperm cell walls	1596:1620	rice endosperm cell walls	1596:1620	Detection of the LM5 galactan epitope showed a clear distinction between wheat and rice, being detected at the earliest stages of development in rice endosperm cell walls, but not detected in wheat endosperm cell walls, only in maternal tissues.
25416597	2	63	theme	specific	227:234	arg1	antibodies					216:225	Monoclonal antibodies	205:225	Monoclonal antibodies specific to cell wall polysaccharides	205:263	Monoclonal antibodies specific to cell wall polysaccharides were used to determine polysaccharide dynamics during the development of both wheat and rice grain.
25416597	6	64	theme	cell	916:919	arg1	outgrowths					926:935	cell wall outgrowths	916:935	cell wall outgrowths	916:935	Callose has been reported to be associated with the formation of cell wall outgrowths during endosperm cellularisation and xyloglucan is here shown to be a component of these anticlinal extensions, occurring transiently in both species.
25416597	9	65	theme	cell	1611:1614	arg1	walls					1616:1620	rice endosperm cell walls	1596:1620	rice endosperm cell walls	1596:1620	Detection of the LM5 galactan epitope showed a clear distinction between wheat and rice, being detected at the earliest stages of development in rice endosperm cell walls, but not detected in wheat endosperm cell walls, only in maternal tissues.
25416597	8	66	theme	cell	1393:1396	arg1	walls					1398:1402	endosperm cell walls	1383:1402	endosperm cell walls from 12 DAA in rice and 20 DAA in wheat grain	1383:1448	A rhamnogalacturonan-I (RG-I) backbone epitope was observed to be temporally regulated in both species, detected in endosperm cell walls from 12 DAA in rice and 20 DAA in wheat grain.
25416597	4	67	theme	cellular	652:659	arg1	locations					661:669	analogous cellular locations	642:669	analogous cellular locations	642:669	Arabinoxylan (AX) and mixed-linkage glucan (MLG) have analogous cellular locations in both species, with deposition of AX and MLG coinciding with the start of grain filling.
25416597	2	68	theme	Monoclonal	205:214	arg1	antibodies					216:225	Monoclonal antibodies	205:225	Monoclonal antibodies specific to cell wall polysaccharides	205:263	Monoclonal antibodies specific to cell wall polysaccharides were used to determine polysaccharide dynamics during the development of both wheat and rice grain.
25416597	1	69	theme	wall	114:117	arg1	polysaccharides					119:133	Cell wall polysaccharides	109:133	Cell wall polysaccharides of wheat and rice endosperm	109:161	Cell wall polysaccharides of wheat and rice endosperm are an important source of dietary fibre.
25416597	1	69	theme	wall	114:117	arg1	source					180:185	an important source	167:185	an important source of dietary fibre	167:202	Cell wall polysaccharides of wheat and rice endosperm are an important source of dietary fibre.
25416597	7	70	theme	grain	1182:1186	arg1	tissues					1156:1162	maternal tissues	1147:1162	maternal tissues of wheat and rice grain	1147:1186	Pectic homogalacturonan (HG) was abundant in cell walls of maternal tissues of wheat and rice grain, but only detected in endosperm cell walls of rice in an unesterified HG form.
25416597	2	71	theme	cell	239:242	arg1	polysaccharides					249:263	cell wall polysaccharides	239:263	cell wall polysaccharides	239:263	Monoclonal antibodies specific to cell wall polysaccharides were used to determine polysaccharide dynamics during the development of both wheat and rice grain.
25416597	9	72	from	walls	1616:1620	arg1	stages					1571:1576	the earliest stages	1558:1576	the earliest stages of development in rice endosperm cell walls	1558:1620	Detection of the LM5 galactan epitope showed a clear distinction between wheat and rice, being detected at the earliest stages of development in rice endosperm cell walls, but not detected in wheat endosperm cell walls, only in maternal tissues.
25416597	9	73	dep	showed	1489:1494	arg1	detected					1631:1638	detected	1631:1638	detected	1631:1638	Detection of the LM5 galactan epitope showed a clear distinction between wheat and rice, being detected at the earliest stages of development in rice endosperm cell walls, but not detected in wheat endosperm cell walls, only in maternal tissues.
25416597	9	73	dep	showed	1489:1494	arg1	detected					1546:1553	detected	1546:1553	detected at the earliest stages of development in rice endosperm cell walls	1546:1620	Detection of the LM5 galactan epitope showed a clear distinction between wheat and rice, being detected at the earliest stages of development in rice endosperm cell walls, but not detected in wheat endosperm cell walls, only in maternal tissues.
25416597	9	74	theme	endosperm	1649:1657	arg1	walls					1664:1668	wheat endosperm cell walls	1643:1668	wheat endosperm cell walls	1643:1668	Detection of the LM5 galactan epitope showed a clear distinction between wheat and rice, being detected at the earliest stages of development in rice endosperm cell walls, but not detected in wheat endosperm cell walls, only in maternal tissues.
25416597	8	75	from	DAA	1412:1414	arg1	DAA					1431:1433	20 DAA	1428:1433	20 DAA	1428:1433	A rhamnogalacturonan-I (RG-I) backbone epitope was observed to be temporally regulated in both species, detected in endosperm cell walls from 12 DAA in rice and 20 DAA in wheat grain.
25416597	8	75	from	DAA	1412:1414	arg1	rice					1419:1422	rice	1419:1422	rice	1419:1422	A rhamnogalacturonan-I (RG-I) backbone epitope was observed to be temporally regulated in both species, detected in endosperm cell walls from 12 DAA in rice and 20 DAA in wheat grain.
25416597	8	75	from	DAA	1412:1414	arg1	walls					1398:1402	endosperm cell walls	1383:1402	endosperm cell walls from 12 DAA in rice and 20 DAA in wheat grain	1383:1448	A rhamnogalacturonan-I (RG-I) backbone epitope was observed to be temporally regulated in both species, detected in endosperm cell walls from 12 DAA in rice and 20 DAA in wheat grain.
25416597	8	76	theme	rhamnogalacturonan-I	1269:1288	arg1	epitope					1306:1312	A rhamnogalacturonan-I (RG-I) backbone epitope	1267:1312	A rhamnogalacturonan-I (RG-I) backbone epitope	1267:1312	A rhamnogalacturonan-I (RG-I) backbone epitope was observed to be temporally regulated in both species, detected in endosperm cell walls from 12 DAA in rice and 20 DAA in wheat grain.
25416597	4	77	theme	filling	753:759	arg1	start					738:742	the start	734:742	the start of grain filling	734:759	Arabinoxylan (AX) and mixed-linkage glucan (MLG) have analogous cellular locations in both species, with deposition of AX and MLG coinciding with the start of grain filling.
25416597	7	78	theme	maternal	1147:1154	arg1	tissues					1156:1162	maternal tissues	1147:1162	maternal tissues of wheat and rice grain	1147:1186	Pectic homogalacturonan (HG) was abundant in cell walls of maternal tissues of wheat and rice grain, but only detected in endosperm cell walls of rice in an unesterified HG form.
25416597	4	79	dep	Arabinoxylan	588:599	arg1	AX					602:603	AX	602:603	AX	602:603	Arabinoxylan (AX) and mixed-linkage glucan (MLG) have analogous cellular locations in both species, with deposition of AX and MLG coinciding with the start of grain filling.
25416597	7	80	from	walls	1138:1142	arg1	abundant					1121:1128	abundant	1121:1128	abundant	1121:1128	Pectic homogalacturonan (HG) was abundant in cell walls of maternal tissues of wheat and rice grain, but only detected in endosperm cell walls of rice in an unesterified HG form.
25416597	8	81	theme	RG-I	1291:1294	arg1	epitope					1306:1312	A rhamnogalacturonan-I (RG-I) backbone epitope	1267:1312	A rhamnogalacturonan-I (RG-I) backbone epitope	1267:1312	A rhamnogalacturonan-I (RG-I) backbone epitope was observed to be temporally regulated in both species, detected in endosperm cell walls from 12 DAA in rice and 20 DAA in wheat grain.
25416597	3	82	theme	different	453:461	arg1	compositions					483:494	significantly different endosperm cell wall compositions	439:494	significantly different endosperm cell wall compositions	439:494	Wheat and rice grain present near synchronous developmental processes and significantly different endosperm cell wall compositions, allowing the localisation of these polysaccharides to be related to developmental changes.
25416597	0	83	theme	in	12:13	arg1	analyses					20:27	Comparative in situ analyses	0:27	Comparative in situ analyses of cell wall matrix polysaccharide dynamics in developing rice and wheat grain.	0:107	Comparative in situ analyses of cell wall matrix polysaccharide dynamics in developing rice and wheat grain.
25416597	9	84	from	stages	1571:1576	arg1	walls					1616:1620	rice endosperm cell walls	1596:1620	rice endosperm cell walls	1596:1620	Detection of the LM5 galactan epitope showed a clear distinction between wheat and rice, being detected at the earliest stages of development in rice endosperm cell walls, but not detected in wheat endosperm cell walls, only in maternal tissues.
25416597	9	85	theme	LM5	1468:1470	arg1	epitope					1481:1487	the LM5 galactan epitope	1464:1487	the LM5 galactan epitope	1464:1487	Detection of the LM5 galactan epitope showed a clear distinction between wheat and rice, being detected at the earliest stages of development in rice endosperm cell walls, but not detected in wheat endosperm cell walls, only in maternal tissues.
25416597	7	86	theme	HG	1258:1259	arg1	form					1261:1264	an unesterified HG form	1242:1264	an unesterified HG form	1242:1264	Pectic homogalacturonan (HG) was abundant in cell walls of maternal tissues of wheat and rice grain, but only detected in endosperm cell walls of rice in an unesterified HG form.
25416597	9	87	theme	maternal	1679:1686	arg1	tissues					1688:1694	maternal tissues	1679:1694	maternal tissues	1679:1694	Detection of the LM5 galactan epitope showed a clear distinction between wheat and rice, being detected at the earliest stages of development in rice endosperm cell walls, but not detected in wheat endosperm cell walls, only in maternal tissues.
25416597	8	88	theme	backbone	1297:1304	arg1	epitope					1306:1312	A rhamnogalacturonan-I (RG-I) backbone epitope	1267:1312	A rhamnogalacturonan-I (RG-I) backbone epitope	1267:1312	A rhamnogalacturonan-I (RG-I) backbone epitope was observed to be temporally regulated in both species, detected in endosperm cell walls from 12 DAA in rice and 20 DAA in wheat grain.
25416597	8	89	from	rice	1419:1422	arg1	grain					1444:1448	wheat grain	1438:1448	wheat grain	1438:1448	A rhamnogalacturonan-I (RG-I) backbone epitope was observed to be temporally regulated in both species, detected in endosperm cell walls from 12 DAA in rice and 20 DAA in wheat grain.
25416597	9	90	theme	epitope	1481:1487	arg1	Detection					1451:1459	Detection	1451:1459	Detection of the LM5 galactan epitope	1451:1487	Detection of the LM5 galactan epitope showed a clear distinction between wheat and rice, being detected at the earliest stages of development in rice endosperm cell walls, but not detected in wheat endosperm cell walls, only in maternal tissues.
25416597	1	91	dep	wheat	138:142	arg1	endosperm					153:161	endosperm	153:161	endosperm	153:161	Cell wall polysaccharides of wheat and rice endosperm are an important source of dietary fibre.
25416597	0	92	theme	cell	32:35	arg1	dynamics					64:71	cell wall matrix polysaccharide dynamics	32:71	cell wall matrix polysaccharide dynamics	32:71	Comparative in situ analyses of cell wall matrix polysaccharide dynamics in developing rice and wheat grain.
25416597	7	93	theme	rice	1234:1237	arg1	walls					1225:1229	endosperm cell walls	1210:1229	endosperm cell walls of rice	1210:1237	Pectic homogalacturonan (HG) was abundant in cell walls of maternal tissues of wheat and rice grain, but only detected in endosperm cell walls of rice in an unesterified HG form.
25416597	3	94	theme	developmental	411:423	arg1	processes					425:433	synchronous developmental processes	399:433	synchronous developmental processes	399:433	Wheat and rice grain present near synchronous developmental processes and significantly different endosperm cell wall compositions, allowing the localisation of these polysaccharides to be related to developmental changes.
25416597	4	95	contain	have	637:640	arg2	locations					661:669	analogous cellular locations	642:669	analogous cellular locations	642:669	Arabinoxylan (AX) and mixed-linkage glucan (MLG) have analogous cellular locations in both species, with deposition of AX and MLG coinciding with the start of grain filling.
25416597	4	95	contain	have	637:640	arg1	Arabinoxylan					588:599	Arabinoxylan	588:599	Arabinoxylan (AX)	588:604	Arabinoxylan (AX) and mixed-linkage glucan (MLG) have analogous cellular locations in both species, with deposition of AX and MLG coinciding with the start of grain filling.
25416597	4	95	contain	have	637:640	arg1	glucan					624:629	mixed-linkage glucan	610:629	mixed-linkage glucan (MLG)	610:635	Arabinoxylan (AX) and mixed-linkage glucan (MLG) have analogous cellular locations in both species, with deposition of AX and MLG coinciding with the start of grain filling.
25416597	4	95	contain	have	637:640	arg1	MLG					632:634	MLG	632:634	MLG	632:634	Arabinoxylan (AX) and mixed-linkage glucan (MLG) have analogous cellular locations in both species, with deposition of AX and MLG coinciding with the start of grain filling.
25416597	3	96	theme	cell	473:476	arg1	compositions					483:494	significantly different endosperm cell wall compositions	439:494	significantly different endosperm cell wall compositions	439:494	Wheat and rice grain present near synchronous developmental processes and significantly different endosperm cell wall compositions, allowing the localisation of these polysaccharides to be related to developmental changes.
25416597	0	97	theme	matrix	42:47	arg1	dynamics					64:71	cell wall matrix polysaccharide dynamics	32:71	cell wall matrix polysaccharide dynamics	32:71	Comparative in situ analyses of cell wall matrix polysaccharide dynamics in developing rice and wheat grain.
25416597	7	98	theme	endosperm	1210:1218	arg1	walls					1225:1229	endosperm cell walls	1210:1229	endosperm cell walls of rice	1210:1237	Pectic homogalacturonan (HG) was abundant in cell walls of maternal tissues of wheat and rice grain, but only detected in endosperm cell walls of rice in an unesterified HG form.
25416597	10	99	located	detected	1739:1746	arg1	contrast					1700:1707	contrast	1700:1707	contrast	1700:1707	In contrast, the LM6 arabinan epitope was detected in both species around 8 DAA and was transient in wheat grain, but persisted in rice until maturity.
25416597	10	99	located	detected	1739:1746	arg2	transient					1785:1793	transient	1785:1793	transient	1785:1793	In contrast, the LM6 arabinan epitope was detected in both species around 8 DAA and was transient in wheat grain, but persisted in rice until maturity.
25416597	10	99	located	detected	1739:1746	arg1	species					1756:1762	both species	1751:1762	both species	1751:1762	In contrast, the LM6 arabinan epitope was detected in both species around 8 DAA and was transient in wheat grain, but persisted in rice until maturity.
25416597	10	99	located	detected	1739:1746	arg2	epitope					1727:1733	the LM6 arabinan epitope	1710:1733	the LM6 arabinan epitope	1710:1733	In contrast, the LM6 arabinan epitope was detected in both species around 8 DAA and was transient in wheat grain, but persisted in rice until maturity.
25416597	1	100	theme	fibre	198:202	arg1	polysaccharides					119:133	Cell wall polysaccharides	109:133	Cell wall polysaccharides of wheat and rice endosperm	109:161	Cell wall polysaccharides of wheat and rice endosperm are an important source of dietary fibre.
25416597	1	100	theme	fibre	198:202	arg1	source					180:185	an important source	167:185	an important source of dietary fibre	167:202	Cell wall polysaccharides of wheat and rice endosperm are an important source of dietary fibre.
25416597	0	101	dep	in	12:13	arg1	situ					15:18	situ	15:18	situ	15:18	Comparative in situ analyses of cell wall matrix polysaccharide dynamics in developing rice and wheat grain.
25162821	0	0	theme	electroconductive	87:103	arg1	properties					123:132	electroconductive and antimicrobial properties	87:132	electroconductive and antimicrobial properties	87:132	Biocomposites of nanofibrillated cellulose, polypyrrole, and silver nanoparticles with electroconductive and antimicrobial properties.
25162821	2	1	theme	good	543:546	arg1	properties					574:583	good mechanical and electrical properties	543:583	good mechanical and electrical properties	543:583	Incorporation of NFC into PPy significantly improved its film formation ability resulting in composite materials with good mechanical and electrical properties.
25162821	4	2	theme	strong	771:776	arg1	activity					792:799	strong antimicrobial activity	771:799	strong antimicrobial activity	771:799	The electrical conductivity and strong antimicrobial activity makes it possible to use the silver composites in various applications aimed at biomedical treatments and diagnostics.
25162821	1	3	theme	free-standing	162:174	arg1	films					186:190	flexible and free-standing composite films	149:190	flexible and free-standing composite films of nanofibrillated cellulose/polypyrrole (NFC/PPy) and NFC/PPy-silver nanoparticles (NFC/PPy-Ag)	149:287	In this work, flexible and free-standing composite films of nanofibrillated cellulose/polypyrrole (NFC/PPy) and NFC/PPy-silver nanoparticles (NFC/PPy-Ag) have been synthesized for the first time via in situ one-step chemical polymerization and applied in potential biomedical applications.
25162821	1	4	theme	composite	176:184	arg1	films					186:190	flexible and free-standing composite films	149:190	flexible and free-standing composite films of nanofibrillated cellulose/polypyrrole (NFC/PPy) and NFC/PPy-silver nanoparticles (NFC/PPy-Ag)	149:287	In this work, flexible and free-standing composite films of nanofibrillated cellulose/polypyrrole (NFC/PPy) and NFC/PPy-silver nanoparticles (NFC/PPy-Ag) have been synthesized for the first time via in situ one-step chemical polymerization and applied in potential biomedical applications.
25162821	3	5	dep	aureus	731:736	arg1	e.g.					710:713	e.g.	710:713	e.g.	710:713	It is shown that the NFC/PPy-Ag composite films have strong inhibition effect against the growth of Gram-positive bacteria, e.g., Staphylococcus aureus.
25162821	0	6	theme	antimicrobial	109:121	arg1	properties					123:132	electroconductive and antimicrobial properties	87:132	electroconductive and antimicrobial properties	87:132	Biocomposites of nanofibrillated cellulose, polypyrrole, and silver nanoparticles with electroconductive and antimicrobial properties.
25162821	5	7	theme	force	1079:1083	arg1	microscopy					1085:1094	atomic force microscopy	1072:1094	atomic force microscopy	1072:1094	Additionally, we report here the structural and morphological characterization of the composite materials with Fourier-transform infrared spectroscopy, atomic force microscopy, and scanning and transmission electron microscopy techniques.
25162821	5	8	theme	atomic	1072:1077	arg1	microscopy					1085:1094	atomic force microscopy	1072:1094	atomic force microscopy	1072:1094	Additionally, we report here the structural and morphological characterization of the composite materials with Fourier-transform infrared spectroscopy, atomic force microscopy, and scanning and transmission electron microscopy techniques.
25162821	5	9	theme	microscopy	1085:1094	arg1	techniques					1147:1156	Fourier-transform infrared spectroscopy, atomic force microscopy, and scanning and transmission electron microscopy techniques	1031:1156	Fourier-transform infrared spectroscopy, atomic force microscopy, and scanning and transmission electron microscopy techniques	1031:1156	Additionally, we report here the structural and morphological characterization of the composite materials with Fourier-transform infrared spectroscopy, atomic force microscopy, and scanning and transmission electron microscopy techniques.
25162821	4	10	from	composites	837:846	arg1	applications					859:870	various applications	851:870	various applications aimed at biomedical treatments and diagnostics	851:917	The electrical conductivity and strong antimicrobial activity makes it possible to use the silver composites in various applications aimed at biomedical treatments and diagnostics.
25162821	4	11	theme	silver	830:835	arg1	composites					837:846	the silver composites	826:846	the silver composites in various applications aimed at biomedical treatments and diagnostics	826:917	The electrical conductivity and strong antimicrobial activity makes it possible to use the silver composites in various applications aimed at biomedical treatments and diagnostics.
25162821	1	12	theme	potential	390:398	arg1	applications					411:422	potential biomedical applications	390:422	potential biomedical applications	390:422	In this work, flexible and free-standing composite films of nanofibrillated cellulose/polypyrrole (NFC/PPy) and NFC/PPy-silver nanoparticles (NFC/PPy-Ag) have been synthesized for the first time via in situ one-step chemical polymerization and applied in potential biomedical applications.
25162821	4	13	theme	various	851:857	arg1	applications					859:870	various applications	851:870	various applications aimed at biomedical treatments and diagnostics	851:917	The electrical conductivity and strong antimicrobial activity makes it possible to use the silver composites in various applications aimed at biomedical treatments and diagnostics.
25162821	5	14	theme	structural	953:962	arg1	characterization					982:997	the structural and morphological characterization	949:997	the structural and morphological characterization of the composite materials with Fourier-transform infrared spectroscopy, atomic force microscopy, and scanning and transmission electron microscopy techniques	949:1156	Additionally, we report here the structural and morphological characterization of the composite materials with Fourier-transform infrared spectroscopy, atomic force microscopy, and scanning and transmission electron microscopy techniques.
25162821	2	15	theme	NFC	442:444	arg1	Incorporation					425:437	Incorporation	425:437	Incorporation of NFC into PPy	425:453	Incorporation of NFC into PPy significantly improved its film formation ability resulting in composite materials with good mechanical and electrical properties.
25162821	2	16	theme	electrical	563:572	arg1	properties					574:583	good mechanical and electrical properties	543:583	good mechanical and electrical properties	543:583	Incorporation of NFC into PPy significantly improved its film formation ability resulting in composite materials with good mechanical and electrical properties.
25162821	1	17	theme	biomedical	400:409	arg1	applications					411:422	potential biomedical applications	390:422	potential biomedical applications	390:422	In this work, flexible and free-standing composite films of nanofibrillated cellulose/polypyrrole (NFC/PPy) and NFC/PPy-silver nanoparticles (NFC/PPy-Ag) have been synthesized for the first time via in situ one-step chemical polymerization and applied in potential biomedical applications.
25162821	1	18	dep	in	334:335	arg1	situ					337:340	situ	337:340	situ	337:340	In this work, flexible and free-standing composite films of nanofibrillated cellulose/polypyrrole (NFC/PPy) and NFC/PPy-silver nanoparticles (NFC/PPy-Ag) have been synthesized for the first time via in situ one-step chemical polymerization and applied in potential biomedical applications.
25162821	5	19	theme	transmission	1114:1125	arg1	microscopy					1136:1145	scanning and transmission electron microscopy	1101:1145	microscopy	1136:1145	Additionally, we report here the structural and morphological characterization of the composite materials with Fourier-transform infrared spectroscopy, atomic force microscopy, and scanning and transmission electron microscopy techniques.
25162821	5	20	theme	infrared	1049:1056	arg1	spectroscopy					1058:1069	Fourier-transform infrared spectroscopy	1031:1069	Fourier-transform infrared spectroscopy	1031:1069	Additionally, we report here the structural and morphological characterization of the composite materials with Fourier-transform infrared spectroscopy, atomic force microscopy, and scanning and transmission electron microscopy techniques.
25162821	1	21	theme	nanofibrillated	195:209	arg1	NFC/PPy					234:240	NFC/PPy	234:240	NFC/PPy	234:240	In this work, flexible and free-standing composite films of nanofibrillated cellulose/polypyrrole (NFC/PPy) and NFC/PPy-silver nanoparticles (NFC/PPy-Ag) have been synthesized for the first time via in situ one-step chemical polymerization and applied in potential biomedical applications.
25162821	1	21	theme	nanofibrillated	195:209	arg1	cellulose/polypyrrole					211:231	nanofibrillated cellulose/polypyrrole	195:231	nanofibrillated cellulose/polypyrrole (NFC/PPy)	195:241	In this work, flexible and free-standing composite films of nanofibrillated cellulose/polypyrrole (NFC/PPy) and NFC/PPy-silver nanoparticles (NFC/PPy-Ag) have been synthesized for the first time via in situ one-step chemical polymerization and applied in potential biomedical applications.
25162821	2	22	theme	formation	487:495	arg1	ability					497:503	its film formation ability	478:503	its film formation ability resulting in composite materials with good mechanical and electrical properties	478:583	Incorporation of NFC into PPy significantly improved its film formation ability resulting in composite materials with good mechanical and electrical properties.
25162821	5	23	with	materials	1016:1024	arg1	techniques					1147:1156	Fourier-transform infrared spectroscopy, atomic force microscopy, and scanning and transmission electron microscopy techniques	1031:1156	Fourier-transform infrared spectroscopy, atomic force microscopy, and scanning and transmission electron microscopy techniques	1031:1156	Additionally, we report here the structural and morphological characterization of the composite materials with Fourier-transform infrared spectroscopy, atomic force microscopy, and scanning and transmission electron microscopy techniques.
25162821	5	24	theme	spectroscopy	1058:1069	arg1	techniques					1147:1156	Fourier-transform infrared spectroscopy, atomic force microscopy, and scanning and transmission electron microscopy techniques	1031:1156	Fourier-transform infrared spectroscopy, atomic force microscopy, and scanning and transmission electron microscopy techniques	1031:1156	Additionally, we report here the structural and morphological characterization of the composite materials with Fourier-transform infrared spectroscopy, atomic force microscopy, and scanning and transmission electron microscopy techniques.
25162821	1	25	theme	cellulose/polypyrrole	211:231	arg1	films					186:190	flexible and free-standing composite films	149:190	flexible and free-standing composite films of nanofibrillated cellulose/polypyrrole (NFC/PPy) and NFC/PPy-silver nanoparticles (NFC/PPy-Ag)	149:287	In this work, flexible and free-standing composite films of nanofibrillated cellulose/polypyrrole (NFC/PPy) and NFC/PPy-silver nanoparticles (NFC/PPy-Ag) have been synthesized for the first time via in situ one-step chemical polymerization and applied in potential biomedical applications.
25162821	0	26	theme	cellulose	33:41	arg1	Biocomposites					0:12	Biocomposites	0:12	Biocomposites of nanofibrillated cellulose, polypyrrole, and silver	0:66	Biocomposites of nanofibrillated cellulose, polypyrrole, and silver nanoparticles with electroconductive and antimicrobial properties.
25162821	4	27	theme	biomedical	881:890	arg1	treatments					892:901	biomedical treatments	881:901	biomedical treatments	881:901	The electrical conductivity and strong antimicrobial activity makes it possible to use the silver composites in various applications aimed at biomedical treatments and diagnostics.
25162821	3	28	theme	NFC/PPy-Ag	607:616	arg1	films					628:632	the NFC/PPy-Ag composite films	603:632	the NFC/PPy-Ag composite films	603:632	It is shown that the NFC/PPy-Ag composite films have strong inhibition effect against the growth of Gram-positive bacteria, e.g., Staphylococcus aureus.
25162821	0	29	theme	nanofibrillated	17:31	arg1	cellulose					33:41	nanofibrillated cellulose	17:41	nanofibrillated cellulose	17:41	Biocomposites of nanofibrillated cellulose, polypyrrole, and silver nanoparticles with electroconductive and antimicrobial properties.
25162821	1	30	theme	first	319:323	arg1	time					325:328	the first time	315:328	the first time	315:328	In this work, flexible and free-standing composite films of nanofibrillated cellulose/polypyrrole (NFC/PPy) and NFC/PPy-silver nanoparticles (NFC/PPy-Ag) have been synthesized for the first time via in situ one-step chemical polymerization and applied in potential biomedical applications.
25162821	3	31	theme	composite	618:626	arg1	films					628:632	the NFC/PPy-Ag composite films	603:632	the NFC/PPy-Ag composite films	603:632	It is shown that the NFC/PPy-Ag composite films have strong inhibition effect against the growth of Gram-positive bacteria, e.g., Staphylococcus aureus.
25162821	5	32	theme	morphological	968:980	arg1	characterization					982:997	the structural and morphological characterization	949:997	the structural and morphological characterization of the composite materials with Fourier-transform infrared spectroscopy, atomic force microscopy, and scanning and transmission electron microscopy techniques	949:1156	Additionally, we report here the structural and morphological characterization of the composite materials with Fourier-transform infrared spectroscopy, atomic force microscopy, and scanning and transmission electron microscopy techniques.
25162821	0	33	theme	polypyrrole	44:54	arg1	Biocomposites					0:12	Biocomposites	0:12	Biocomposites of nanofibrillated cellulose, polypyrrole, and silver	0:66	Biocomposites of nanofibrillated cellulose, polypyrrole, and silver nanoparticles with electroconductive and antimicrobial properties.
25162821	5	34	theme	materials	1016:1024	arg1	characterization					982:997	the structural and morphological characterization	949:997	the structural and morphological characterization of the composite materials with Fourier-transform infrared spectroscopy, atomic force microscopy, and scanning and transmission electron microscopy techniques	949:1156	Additionally, we report here the structural and morphological characterization of the composite materials with Fourier-transform infrared spectroscopy, atomic force microscopy, and scanning and transmission electron microscopy techniques.
25162821	5	35	theme	microscopy	1136:1145	arg1	techniques					1147:1156	Fourier-transform infrared spectroscopy, atomic force microscopy, and scanning and transmission electron microscopy techniques	1031:1156	Fourier-transform infrared spectroscopy, atomic force microscopy, and scanning and transmission electron microscopy techniques	1031:1156	Additionally, we report here the structural and morphological characterization of the composite materials with Fourier-transform infrared spectroscopy, atomic force microscopy, and scanning and transmission electron microscopy techniques.
25162821	2	36	theme	composite	518:526	arg1	materials					528:536	composite materials	518:536	composite materials with good mechanical and electrical properties	518:583	Incorporation of NFC into PPy significantly improved its film formation ability resulting in composite materials with good mechanical and electrical properties.
25162821	3	37	theme	bacteria	700:707	arg1	growth					676:681	the growth	672:681	the growth of Gram-positive bacteria, e.g., Staphylococcus aureus	672:736	It is shown that the NFC/PPy-Ag composite films have strong inhibition effect against the growth of Gram-positive bacteria, e.g., Staphylococcus aureus.
25162821	2	38	theme	film	482:485	arg1	ability					497:503	its film formation ability	478:503	its film formation ability resulting in composite materials with good mechanical and electrical properties	478:583	Incorporation of NFC into PPy significantly improved its film formation ability resulting in composite materials with good mechanical and electrical properties.
25162821	1	39	theme	in	334:335	arg1	polymerization					360:373	in situ one-step chemical polymerization	334:373	in situ one-step chemical polymerization	334:373	In this work, flexible and free-standing composite films of nanofibrillated cellulose/polypyrrole (NFC/PPy) and NFC/PPy-silver nanoparticles (NFC/PPy-Ag) have been synthesized for the first time via in situ one-step chemical polymerization and applied in potential biomedical applications.
25162821	2	40	theme	mechanical	548:557	arg1	properties					574:583	good mechanical and electrical properties	543:583	good mechanical and electrical properties	543:583	Incorporation of NFC into PPy significantly improved its film formation ability resulting in composite materials with good mechanical and electrical properties.
25162821	3	41	theme	strong	639:644	arg1	effect					657:662	strong inhibition effect	639:662	strong inhibition effect	639:662	It is shown that the NFC/PPy-Ag composite films have strong inhibition effect against the growth of Gram-positive bacteria, e.g., Staphylococcus aureus.
25162821	5	42	theme	composite	1006:1014	arg1	materials					1016:1024	the composite materials	1002:1024	the composite materials with Fourier-transform infrared spectroscopy, atomic force microscopy, and scanning and transmission electron microscopy techniques	1002:1156	Additionally, we report here the structural and morphological characterization of the composite materials with Fourier-transform infrared spectroscopy, atomic force microscopy, and scanning and transmission electron microscopy techniques.
25162821	3	43	theme	inhibition	646:655	arg1	effect					657:662	strong inhibition effect	639:662	strong inhibition effect	639:662	It is shown that the NFC/PPy-Ag composite films have strong inhibition effect against the growth of Gram-positive bacteria, e.g., Staphylococcus aureus.
25162821	4	44	theme	antimicrobial	778:790	arg1	activity					792:799	strong antimicrobial activity	771:799	strong antimicrobial activity	771:799	The electrical conductivity and strong antimicrobial activity makes it possible to use the silver composites in various applications aimed at biomedical treatments and diagnostics.
25162821	1	45	theme	one-step	342:349	arg1	polymerization					360:373	in situ one-step chemical polymerization	334:373	in situ one-step chemical polymerization	334:373	In this work, flexible and free-standing composite films of nanofibrillated cellulose/polypyrrole (NFC/PPy) and NFC/PPy-silver nanoparticles (NFC/PPy-Ag) have been synthesized for the first time via in situ one-step chemical polymerization and applied in potential biomedical applications.
25162821	5	46	theme	scanning	1101:1108	arg1	microscopy					1136:1145	scanning and transmission electron microscopy	1101:1145	microscopy	1136:1145	Additionally, we report here the structural and morphological characterization of the composite materials with Fourier-transform infrared spectroscopy, atomic force microscopy, and scanning and transmission electron microscopy techniques.
25162821	1	47	theme	NFC/PPy-silver	247:260	arg1	NFC/PPy-Ag					277:286	NFC/PPy-Ag	277:286	NFC/PPy-Ag	277:286	In this work, flexible and free-standing composite films of nanofibrillated cellulose/polypyrrole (NFC/PPy) and NFC/PPy-silver nanoparticles (NFC/PPy-Ag) have been synthesized for the first time via in situ one-step chemical polymerization and applied in potential biomedical applications.
25162821	1	47	theme	NFC/PPy-silver	247:260	arg1	nanoparticles					262:274	NFC/PPy-silver nanoparticles	247:274	NFC/PPy-silver nanoparticles (NFC/PPy-Ag)	247:287	In this work, flexible and free-standing composite films of nanofibrillated cellulose/polypyrrole (NFC/PPy) and NFC/PPy-silver nanoparticles (NFC/PPy-Ag) have been synthesized for the first time via in situ one-step chemical polymerization and applied in potential biomedical applications.
25162821	3	48	theme	Gram-positive	686:698	arg1	bacteria					700:707	Gram-positive bacteria	686:707	Gram-positive bacteria	686:707	It is shown that the NFC/PPy-Ag composite films have strong inhibition effect against the growth of Gram-positive bacteria, e.g., Staphylococcus aureus.
25162821	5	49	theme	Fourier-transform	1031:1047	arg1	spectroscopy					1058:1069	Fourier-transform infrared spectroscopy	1031:1069	Fourier-transform infrared spectroscopy	1031:1069	Additionally, we report here the structural and morphological characterization of the composite materials with Fourier-transform infrared spectroscopy, atomic force microscopy, and scanning and transmission electron microscopy techniques.
25162821	1	50	theme	nanoparticles	262:274	arg1	films					186:190	flexible and free-standing composite films	149:190	flexible and free-standing composite films of nanofibrillated cellulose/polypyrrole (NFC/PPy) and NFC/PPy-silver nanoparticles (NFC/PPy-Ag)	149:287	In this work, flexible and free-standing composite films of nanofibrillated cellulose/polypyrrole (NFC/PPy) and NFC/PPy-silver nanoparticles (NFC/PPy-Ag) have been synthesized for the first time via in situ one-step chemical polymerization and applied in potential biomedical applications.
25162821	3	51	contain	have	634:637	arg1	films					628:632	the NFC/PPy-Ag composite films	603:632	the NFC/PPy-Ag composite films	603:632	It is shown that the NFC/PPy-Ag composite films have strong inhibition effect against the growth of Gram-positive bacteria, e.g., Staphylococcus aureus.
25162821	3	51	contain	have	634:637	arg2	effect					657:662	strong inhibition effect	639:662	strong inhibition effect	639:662	It is shown that the NFC/PPy-Ag composite films have strong inhibition effect against the growth of Gram-positive bacteria, e.g., Staphylococcus aureus.
25162821	1	52	theme	flexible	149:156	arg1	films					186:190	flexible and free-standing composite films	149:190	flexible and free-standing composite films of nanofibrillated cellulose/polypyrrole (NFC/PPy) and NFC/PPy-silver nanoparticles (NFC/PPy-Ag)	149:287	In this work, flexible and free-standing composite films of nanofibrillated cellulose/polypyrrole (NFC/PPy) and NFC/PPy-silver nanoparticles (NFC/PPy-Ag) have been synthesized for the first time via in situ one-step chemical polymerization and applied in potential biomedical applications.
25162821	0	53	theme	silver	61:66	arg1	Biocomposites					0:12	Biocomposites	0:12	Biocomposites of nanofibrillated cellulose, polypyrrole, and silver	0:66	Biocomposites of nanofibrillated cellulose, polypyrrole, and silver nanoparticles with electroconductive and antimicrobial properties.
25162821	1	54	theme	chemical	351:358	arg1	polymerization					360:373	in situ one-step chemical polymerization	334:373	in situ one-step chemical polymerization	334:373	In this work, flexible and free-standing composite films of nanofibrillated cellulose/polypyrrole (NFC/PPy) and NFC/PPy-silver nanoparticles (NFC/PPy-Ag) have been synthesized for the first time via in situ one-step chemical polymerization and applied in potential biomedical applications.
25162821	5	55	theme	electron	1127:1134	arg1	microscopy					1136:1145	scanning and transmission electron microscopy	1101:1145	microscopy	1136:1145	Additionally, we report here the structural and morphological characterization of the composite materials with Fourier-transform infrared spectroscopy, atomic force microscopy, and scanning and transmission electron microscopy techniques.
25162821	2	56	with	materials	528:536	arg1	properties					574:583	good mechanical and electrical properties	543:583	good mechanical and electrical properties	543:583	Incorporation of NFC into PPy significantly improved its film formation ability resulting in composite materials with good mechanical and electrical properties.
25162821	4	57	theme	electrical	743:752	arg1	conductivity					754:765	The electrical conductivity	739:765	The electrical conductivity	739:765	The electrical conductivity and strong antimicrobial activity makes it possible to use the silver composites in various applications aimed at biomedical treatments and diagnostics.
27987991	4	0	dep	respectively	739:750	arg1	p<0.05					753:758	p<0.05	753:758	p<0.05	753:758	AG incorporation greatly reduced the water vapor permeability (WVP) and solubility of gelatin films, as this decline for the blend film with a 50:50 ratio of biopolymers has been about 41% and 66%, respectively (p<0.05).
27987991	7	1	theme	films	1070:1074	arg1	properties					1040:1049	the properties	1036:1049	the properties of FG/AG bio-based films	1036:1074	Therefore, the blend composition influenced the properties of FG/AG bio-based films.
27987991	6	2	theme	microscopy	933:942	arg1	images					984:989	scanning electron microscopy (SEM) and atomic force microscopy (AFM) images	915:989	scanning electron microscopy (SEM) and atomic force microscopy (AFM) images	915:989	Both polymers showed good compatibility, as demonstrated by scanning electron microscopy (SEM) and atomic force microscopy (AFM) images.
27987991	4	3	theme	blend	666:670	arg1	film					672:675	the blend film	662:675	the blend film	662:675	AG incorporation greatly reduced the water vapor permeability (WVP) and solubility of gelatin films, as this decline for the blend film with a 50:50 ratio of biopolymers has been about 41% and 66%, respectively (p<0.05).
27987991	4	4	theme	biopolymers	699:709	arg1	ratio					690:694	a 50:50 ratio	682:694	a 50:50 ratio of biopolymers	682:709	AG incorporation greatly reduced the water vapor permeability (WVP) and solubility of gelatin films, as this decline for the blend film with a 50:50 ratio of biopolymers has been about 41% and 66%, respectively (p<0.05).
27987991	5	5	theme	FG	803:804	arg1	films					806:810	FG films	803:810	FG films	803:810	Additional advantages of AG inclusion to FG films are the reduction of the UV-transmittance.
27987991	2	6	dep	100:0	315:319	arg1	AG					354:355	AG	354:355	AG	354:355	For this purpose, FG was blended with agar (AG) in different compositions to acquire biodegradable films (100:0, 80:20, 60:40, 50:50 & 0:100, FG:AG).
27987991	7	7	theme	blend	1007:1011	arg1	composition					1013:1023	the blend composition	1003:1023	the blend composition	1003:1023	Therefore, the blend composition influenced the properties of FG/AG bio-based films.
27987991	6	8	theme	electron	924:931	arg1	SEM					945:947	SEM	945:947	SEM	945:947	Both polymers showed good compatibility, as demonstrated by scanning electron microscopy (SEM) and atomic force microscopy (AFM) images.
27987991	6	8	theme	electron	924:931	arg1	microscopy					933:942	scanning electron microscopy	915:942	scanning electron microscopy (SEM)	915:948	Both polymers showed good compatibility, as demonstrated by scanning electron microscopy (SEM) and atomic force microscopy (AFM) images.
27987991	1	9	theme	fish	178:181	arg1	gelatin					183:189	fish gelatin	178:189	fish gelatin	178:189	This study was conducted with the aim of improving the physico-chemical properties of fish gelatin (FG) based films.
27987991	6	10	theme	atomic	954:959	arg1	AFM					979:981	AFM	979:981	AFM	979:981	Both polymers showed good compatibility, as demonstrated by scanning electron microscopy (SEM) and atomic force microscopy (AFM) images.
27987991	6	10	theme	atomic	954:959	arg1	microscopy					967:976	atomic force microscopy	954:976	atomic force microscopy (AFM)	954:982	Both polymers showed good compatibility, as demonstrated by scanning electron microscopy (SEM) and atomic force microscopy (AFM) images.
27987991	2	11	theme	50:50	336:340	arg1	100:0					315:319	100:0	315:319	100:0	315:319	For this purpose, FG was blended with agar (AG) in different compositions to acquire biodegradable films (100:0, 80:20, 60:40, 50:50 & 0:100, FG:AG).
27987991	2	11	theme	50:50	336:340	arg1	0:100					344:348	50:50 & 0:100	336:348	50:50 & 0:100	336:348	For this purpose, FG was blended with agar (AG) in different compositions to acquire biodegradable films (100:0, 80:20, 60:40, 50:50 & 0:100, FG:AG).
27987991	4	12	theme	films	635:639	arg1	WVP					604:606	WVP	604:606	WVP	604:606	AG incorporation greatly reduced the water vapor permeability (WVP) and solubility of gelatin films, as this decline for the blend film with a 50:50 ratio of biopolymers has been about 41% and 66%, respectively (p<0.05).
27987991	4	12	theme	films	635:639	arg1	solubility					613:622	solubility	613:622	solubility of gelatin films	613:639	AG incorporation greatly reduced the water vapor permeability (WVP) and solubility of gelatin films, as this decline for the blend film with a 50:50 ratio of biopolymers has been about 41% and 66%, respectively (p<0.05).
27987991	4	12	theme	films	635:639	arg1	permeability					590:601	the water vapor permeability	574:601	the water vapor permeability (WVP)	574:607	AG incorporation greatly reduced the water vapor permeability (WVP) and solubility of gelatin films, as this decline for the blend film with a 50:50 ratio of biopolymers has been about 41% and 66%, respectively (p<0.05).
27987991	4	13	theme	water	578:582	arg1	WVP					604:606	WVP	604:606	WVP	604:606	AG incorporation greatly reduced the water vapor permeability (WVP) and solubility of gelatin films, as this decline for the blend film with a 50:50 ratio of biopolymers has been about 41% and 66%, respectively (p<0.05).
27987991	4	13	theme	water	578:582	arg1	permeability					590:601	the water vapor permeability	574:601	the water vapor permeability (WVP)	574:607	AG incorporation greatly reduced the water vapor permeability (WVP) and solubility of gelatin films, as this decline for the blend film with a 50:50 ratio of biopolymers has been about 41% and 66%, respectively (p<0.05).
27987991	0	14	theme	Physico-chemical	0:15	arg1	properties					37:46	Physico-chemical and microstructural properties	0:46	Physico-chemical and microstructural properties of fish	0:54	Physico-chemical and microstructural properties of fish gelatin/agar bio-based blend films.
27987991	4	15	theme	gelatin	627:633	arg1	films					635:639	gelatin films	627:639	gelatin films	627:639	AG incorporation greatly reduced the water vapor permeability (WVP) and solubility of gelatin films, as this decline for the blend film with a 50:50 ratio of biopolymers has been about 41% and 66%, respectively (p<0.05).
27987991	2	16	theme	different	260:268	arg1	compositions					270:281	different compositions	260:281	different compositions	260:281	For this purpose, FG was blended with agar (AG) in different compositions to acquire biodegradable films (100:0, 80:20, 60:40, 50:50 & 0:100, FG:AG).
27987991	0	17	theme	microstructural	21:35	arg1	properties					37:46	Physico-chemical and microstructural properties	0:46	Physico-chemical and microstructural properties of fish	0:54	Physico-chemical and microstructural properties of fish gelatin/agar bio-based blend films.
27987991	4	18	with	decline	650:656	arg1	ratio					690:694	a 50:50 ratio	682:694	a 50:50 ratio of biopolymers	682:709	AG incorporation greatly reduced the water vapor permeability (WVP) and solubility of gelatin films, as this decline for the blend film with a 50:50 ratio of biopolymers has been about 41% and 66%, respectively (p<0.05).
27987991	5	19	theme	AG	787:788	arg1	inclusion					790:798	AG inclusion	787:798	AG inclusion	787:798	Additional advantages of AG inclusion to FG films are the reduction of the UV-transmittance.
27987991	2	20	dep	films	308:312	arg1	FG					351:352	FG	351:352	FG	351:352	For this purpose, FG was blended with agar (AG) in different compositions to acquire biodegradable films (100:0, 80:20, 60:40, 50:50 & 0:100, FG:AG).
27987991	2	20	dep	films	308:312	arg1	60:40					329:333	60:40	329:333	60:40	329:333	For this purpose, FG was blended with agar (AG) in different compositions to acquire biodegradable films (100:0, 80:20, 60:40, 50:50 & 0:100, FG:AG).
27987991	2	20	dep	films	308:312	arg1	80:20					322:326	80:20	322:326	80:20	322:326	For this purpose, FG was blended with agar (AG) in different compositions to acquire biodegradable films (100:0, 80:20, 60:40, 50:50 & 0:100, FG:AG).
27987991	2	20	dep	films	308:312	arg1	0:100					344:348	50:50 & 0:100	336:348	50:50 & 0:100	336:348	For this purpose, FG was blended with agar (AG) in different compositions to acquire biodegradable films (100:0, 80:20, 60:40, 50:50 & 0:100, FG:AG).
27987991	2	20	dep	films	308:312	arg1	100:0					315:319	100:0	315:319	100:0	315:319	For this purpose, FG was blended with agar (AG) in different compositions to acquire biodegradable films (100:0, 80:20, 60:40, 50:50 & 0:100, FG:AG).
27987991	1	21	theme	based	196:200	arg1	films					202:206	fish gelatin (FG) based films	178:206	fish gelatin (FG) based films	178:206	This study was conducted with the aim of improving the physico-chemical properties of fish gelatin (FG) based films.
27987991	0	22	theme	fish	51:54	arg1	properties					37:46	Physico-chemical and microstructural properties	0:46	Physico-chemical and microstructural properties of fish	0:54	Physico-chemical and microstructural properties of fish gelatin/agar bio-based blend films.
27987991	5	23	theme	UV-transmittance	837:852	arg1	reduction					820:828	the reduction	816:828	the reduction of the UV-transmittance	816:852	Additional advantages of AG inclusion to FG films are the reduction of the UV-transmittance.
27987991	5	23	theme	UV-transmittance	837:852	arg1	advantages					773:782	Additional advantages	762:782	Additional advantages of AG inclusion to FG films	762:810	Additional advantages of AG inclusion to FG films are the reduction of the UV-transmittance.
27987991	1	24	theme	films	202:206	arg1	properties					164:173	the physico-chemical properties	143:173	the physico-chemical properties of fish gelatin (FG) based films	143:206	This study was conducted with the aim of improving the physico-chemical properties of fish gelatin (FG) based films.
27987991	3	25	theme	obtained	363:370	arg1	results					372:378	The obtained results	359:378	The obtained results	359:378	The obtained results showed that the AG addition strongly increased the film rigidity and resistance to fracture, while reducing the film stretchability, mainly at 50FG: 50AG ratio.
27987991	2	26	theme	&	342:342	arg1	100:0					315:319	100:0	315:319	100:0	315:319	For this purpose, FG was blended with agar (AG) in different compositions to acquire biodegradable films (100:0, 80:20, 60:40, 50:50 & 0:100, FG:AG).
27987991	2	26	theme	&	342:342	arg1	0:100					344:348	50:50 & 0:100	336:348	50:50 & 0:100	336:348	For this purpose, FG was blended with agar (AG) in different compositions to acquire biodegradable films (100:0, 80:20, 60:40, 50:50 & 0:100, FG:AG).
27987991	4	27	theme	50:50	684:688	arg1	ratio					690:694	a 50:50 ratio	682:694	a 50:50 ratio of biopolymers	682:709	AG incorporation greatly reduced the water vapor permeability (WVP) and solubility of gelatin films, as this decline for the blend film with a 50:50 ratio of biopolymers has been about 41% and 66%, respectively (p<0.05).
27987991	4	28	theme	AG	541:542	arg1	incorporation					544:556	AG incorporation	541:556	AG incorporation	541:556	AG incorporation greatly reduced the water vapor permeability (WVP) and solubility of gelatin films, as this decline for the blend film with a 50:50 ratio of biopolymers has been about 41% and 66%, respectively (p<0.05).
27987991	0	29	theme	bio-based	69:77	arg1	films					85:89	bio-based blend films	69:89	bio-based blend films	69:89	Physico-chemical and microstructural properties of fish gelatin/agar bio-based blend films.
27987991	7	30	theme	bio-based	1060:1068	arg1	films					1070:1074	FG/AG bio-based films	1054:1074	FG/AG bio-based films	1054:1074	Therefore, the blend composition influenced the properties of FG/AG bio-based films.
27987991	3	31	theme	film	492:495	arg1	stretchability					497:510	the film stretchability	488:510	the film stretchability	488:510	The obtained results showed that the AG addition strongly increased the film rigidity and resistance to fracture, while reducing the film stretchability, mainly at 50FG: 50AG ratio.
27987991	6	32	theme	microscopy	967:976	arg1	images					984:989	scanning electron microscopy (SEM) and atomic force microscopy (AFM) images	915:989	scanning electron microscopy (SEM) and atomic force microscopy (AFM) images	915:989	Both polymers showed good compatibility, as demonstrated by scanning electron microscopy (SEM) and atomic force microscopy (AFM) images.
27987991	6	33	theme	force	961:965	arg1	AFM					979:981	AFM	979:981	AFM	979:981	Both polymers showed good compatibility, as demonstrated by scanning electron microscopy (SEM) and atomic force microscopy (AFM) images.
27987991	6	33	theme	force	961:965	arg1	microscopy					967:976	atomic force microscopy	954:976	atomic force microscopy (AFM)	954:982	Both polymers showed good compatibility, as demonstrated by scanning electron microscopy (SEM) and atomic force microscopy (AFM) images.
27987991	1	34	dep	based	196:200	arg1	gelatin					183:189	fish gelatin	178:189	fish gelatin	178:189	This study was conducted with the aim of improving the physico-chemical properties of fish gelatin (FG) based films.
27987991	4	35	theme	vapor	584:588	arg1	WVP					604:606	WVP	604:606	WVP	604:606	AG incorporation greatly reduced the water vapor permeability (WVP) and solubility of gelatin films, as this decline for the blend film with a 50:50 ratio of biopolymers has been about 41% and 66%, respectively (p<0.05).
27987991	4	35	theme	vapor	584:588	arg1	permeability					590:601	the water vapor permeability	574:601	the water vapor permeability (WVP)	574:607	AG incorporation greatly reduced the water vapor permeability (WVP) and solubility of gelatin films, as this decline for the blend film with a 50:50 ratio of biopolymers has been about 41% and 66%, respectively (p<0.05).
27987991	2	36	theme	biodegradable	294:306	arg1	films					308:312	biodegradable films	294:312	biodegradable films (100:0, 80:20, 60:40, 50:50 & 0:100, FG:AG)	294:356	For this purpose, FG was blended with agar (AG) in different compositions to acquire biodegradable films (100:0, 80:20, 60:40, 50:50 & 0:100, FG:AG).
27987991	5	37	theme	inclusion	790:798	arg1	reduction					820:828	the reduction	816:828	the reduction of the UV-transmittance	816:852	Additional advantages of AG inclusion to FG films are the reduction of the UV-transmittance.
27987991	5	37	theme	inclusion	790:798	arg1	advantages					773:782	Additional advantages	762:782	Additional advantages of AG inclusion to FG films	762:810	Additional advantages of AG inclusion to FG films are the reduction of the UV-transmittance.
27987991	3	38	theme	50AG	529:532	arg1	ratio					534:538	50FG: 50AG ratio	523:538	50FG: 50AG ratio	523:538	The obtained results showed that the AG addition strongly increased the film rigidity and resistance to fracture, while reducing the film stretchability, mainly at 50FG: 50AG ratio.
27987991	3	39	theme	AG	396:397	arg1	addition					399:406	the AG addition	392:406	the AG addition	392:406	The obtained results showed that the AG addition strongly increased the film rigidity and resistance to fracture, while reducing the film stretchability, mainly at 50FG: 50AG ratio.
27987991	5	40	theme	Additional	762:771	arg1	reduction					820:828	the reduction	816:828	the reduction of the UV-transmittance	816:852	Additional advantages of AG inclusion to FG films are the reduction of the UV-transmittance.
27987991	5	40	theme	Additional	762:771	arg1	advantages					773:782	Additional advantages	762:782	Additional advantages of AG inclusion to FG films	762:810	Additional advantages of AG inclusion to FG films are the reduction of the UV-transmittance.
27987991	7	41	theme	FG/AG	1054:1058	arg1	films					1070:1074	FG/AG bio-based films	1054:1074	FG/AG bio-based films	1054:1074	Therefore, the blend composition influenced the properties of FG/AG bio-based films.
27987991	6	42	theme	good	876:879	arg1	compatibility					881:893	good compatibility	876:893	good compatibility	876:893	Both polymers showed good compatibility, as demonstrated by scanning electron microscopy (SEM) and atomic force microscopy (AFM) images.
27987991	3	43	theme	film	431:434	arg1	rigidity					436:443	the film rigidity and resistance	427:458	rigidity	436:443	The obtained results showed that the AG addition strongly increased the film rigidity and resistance to fracture, while reducing the film stretchability, mainly at 50FG: 50AG ratio.
27987991	6	44	theme	scanning	915:922	arg1	SEM					945:947	SEM	945:947	SEM	945:947	Both polymers showed good compatibility, as demonstrated by scanning electron microscopy (SEM) and atomic force microscopy (AFM) images.
27987991	6	44	theme	scanning	915:922	arg1	microscopy					933:942	scanning electron microscopy	915:942	scanning electron microscopy (SEM)	915:948	Both polymers showed good compatibility, as demonstrated by scanning electron microscopy (SEM) and atomic force microscopy (AFM) images.
27987991	0	45	theme	blend	79:83	arg1	films					85:89	bio-based blend films	69:89	bio-based blend films	69:89	Physico-chemical and microstructural properties of fish gelatin/agar bio-based blend films.
27987991	3	46	theme	50FG	523:526	arg1	ratio					534:538	50FG: 50AG ratio	523:538	50FG: 50AG ratio	523:538	The obtained results showed that the AG addition strongly increased the film rigidity and resistance to fracture, while reducing the film stretchability, mainly at 50FG: 50AG ratio.
27987991	1	47	theme	physico-chemical	147:162	arg1	properties					164:173	the physico-chemical properties	143:173	the physico-chemical properties of fish gelatin (FG) based films	143:206	This study was conducted with the aim of improving the physico-chemical properties of fish gelatin (FG) based films.
28741860	5	0	dep	increase	1075:1082	arg1	times					1069:1073	times	1069:1073	times	1069:1073	Upon exposure to 3OC12 HSL, the embedded bacteria exhibit an up to 12 ± 3 times increase in fluorescence intensity due to autoinducer-triggered GFP expression.
28741860	3	1	dep	four-fold	708:716	arg1	more					698:701	more	698:701	more	698:701	The different assembly mechanisms are shown not to interfere with each other and despite the more than four-fold increased moduli of the hydrogels, diffusion of autoinducers into the gels remains efficient and Escherichia coli pLuxR-green fluorescent protein (GFP) reporter bacteria are proliferating.
28741860	3	1	dep	four-fold	708:716	arg1	than					703:706	than	703:706	than	703:706	The different assembly mechanisms are shown not to interfere with each other and despite the more than four-fold increased moduli of the hydrogels, diffusion of autoinducers into the gels remains efficient and Escherichia coli pLuxR-green fluorescent protein (GFP) reporter bacteria are proliferating.
28741860	4	2	theme	bacteria-loaded	935:949	arg1	hydrogels					951:959	reporter bacteria-loaded hydrogels	926:959	reporter bacteria-loaded hydrogels	926:959	Templating affords reporter bacteria-loaded hydrogels with controllable shape and size.
28741860	2	3	with	combination	530:540	arg1	crosslinking					591:602	Ca2+ mediated alginate crosslinking	568:602	Ca2+ mediated alginate crosslinking	568:602	For reinforcing hydrogels of 1,4-bi(phenylalanine-diglycol)-benzene (PDB), a hybrid hydrogel is formed by the combination of PDB self-assembly with Ca2+ mediated alginate crosslinking.
28741860	5	4	theme	autoinducer-triggered	1117:1137	arg1	expression					1143:1152	autoinducer-triggered GFP expression	1117:1152	autoinducer-triggered GFP expression	1117:1152	Upon exposure to 3OC12 HSL, the embedded bacteria exhibit an up to 12 ± 3 times increase in fluorescence intensity due to autoinducer-triggered GFP expression.
28741860	2	5	theme	mediated	573:580	arg1	crosslinking					591:602	Ca2+ mediated alginate crosslinking	568:602	Ca2+ mediated alginate crosslinking	568:602	For reinforcing hydrogels of 1,4-bi(phenylalanine-diglycol)-benzene (PDB), a hybrid hydrogel is formed by the combination of PDB self-assembly with Ca2+ mediated alginate crosslinking.
28741860	3	6	theme	hydrogels	742:750	arg1	moduli					728:733	the more than four-fold increased moduli	694:733	the more than four-fold increased moduli of the hydrogels	694:750	The different assembly mechanisms are shown not to interfere with each other and despite the more than four-fold increased moduli of the hydrogels, diffusion of autoinducers into the gels remains efficient and Escherichia coli pLuxR-green fluorescent protein (GFP) reporter bacteria are proliferating.
28741860	4	7	theme	controllable	966:977	arg1	shape					979:983	controllable shape	966:983	controllable shape	966:983	Templating affords reporter bacteria-loaded hydrogels with controllable shape and size.
28741860	6	8	theme	secreted	1267:1274	arg1	autoinducers					1276:1287	their secreted autoinducers	1261:1287	their secreted autoinducers	1261:1287	This approach can serve as a potentially generally applicable strategy to sensitively detect bacteria via their secreted autoinducers.
28741860	5	9	theme	embedded	1027:1034	arg1	bacteria					1036:1043	the embedded bacteria	1023:1043	the embedded bacteria	1023:1043	Upon exposure to 3OC12 HSL, the embedded bacteria exhibit an up to 12 ± 3 times increase in fluorescence intensity due to autoinducer-triggered GFP expression.
28741860	3	10	theme	reporter	870:877	arg1	bacteria					879:886	efficient and Escherichia coli pLuxR-green fluorescent protein (GFP) reporter bacteria	801:886	efficient and Escherichia coli pLuxR-green fluorescent protein (GFP) reporter bacteria	801:886	The different assembly mechanisms are shown not to interfere with each other and despite the more than four-fold increased moduli of the hydrogels, diffusion of autoinducers into the gels remains efficient and Escherichia coli pLuxR-green fluorescent protein (GFP) reporter bacteria are proliferating.
28741860	2	11	theme	Ca2+	568:571	arg1	crosslinking					591:602	Ca2+ mediated alginate crosslinking	568:602	Ca2+ mediated alginate crosslinking	568:602	For reinforcing hydrogels of 1,4-bi(phenylalanine-diglycol)-benzene (PDB), a hybrid hydrogel is formed by the combination of PDB self-assembly with Ca2+ mediated alginate crosslinking.
28741860	3	12	dep	Escherichia	815:825	arg1	coli					827:830	coli	827:830	coli	827:830	The different assembly mechanisms are shown not to interfere with each other and despite the more than four-fold increased moduli of the hydrogels, diffusion of autoinducers into the gels remains efficient and Escherichia coli pLuxR-green fluorescent protein (GFP) reporter bacteria are proliferating.
28741860	1	13	theme	N-acyl	329:334	arg1	lactone					347:353	N-acyl homoserine lactone	329:353	N-acyl homoserine lactone autoinducers (3OC12 HSL)	329:378	A generally applicable strategy to obtain mechanically robust hydrogels for the incorporation and containment of functional reporter bacteria for the microarray and microparticle-based detection and signaling of N-acyl homoserine lactone autoinducers (3OC12 HSL) at relevant concentrations is reported.
28741860	5	14	dep	times	1069:1073	arg1	±					1065:1065	±	1065:1065	±	1065:1065	Upon exposure to 3OC12 HSL, the embedded bacteria exhibit an up to 12 ± 3 times increase in fluorescence intensity due to autoinducer-triggered GFP expression.
28741860	5	14	dep	times	1069:1073	arg1	12					1062:1063	12	1062:1063	12	1062:1063	Upon exposure to 3OC12 HSL, the embedded bacteria exhibit an up to 12 ± 3 times increase in fluorescence intensity due to autoinducer-triggered GFP expression.
28741860	2	15	theme	phenylalanine-diglycol	456:477	arg1	-benzene					479:486	1,4-bi(phenylalanine-diglycol)-benzene	449:486	1,4-bi(phenylalanine-diglycol)-benzene (PDB)	449:492	For reinforcing hydrogels of 1,4-bi(phenylalanine-diglycol)-benzene (PDB), a hybrid hydrogel is formed by the combination of PDB self-assembly with Ca2+ mediated alginate crosslinking.
28741860	2	15	theme	phenylalanine-diglycol	456:477	arg1	PDB					489:491	PDB	489:491	PDB	489:491	For reinforcing hydrogels of 1,4-bi(phenylalanine-diglycol)-benzene (PDB), a hybrid hydrogel is formed by the combination of PDB self-assembly with Ca2+ mediated alginate crosslinking.
28741860	2	16	theme	self-assembly	549:561	arg1	combination					530:540	the combination	526:540	the combination of PDB self-assembly with Ca2+ mediated alginate crosslinking	526:602	For reinforcing hydrogels of 1,4-bi(phenylalanine-diglycol)-benzene (PDB), a hybrid hydrogel is formed by the combination of PDB self-assembly with Ca2+ mediated alginate crosslinking.
28741860	2	17	theme	hybrid	497:502	arg1	hydrogel					504:511	a hybrid hydrogel	495:511	a hybrid hydrogel	495:511	For reinforcing hydrogels of 1,4-bi(phenylalanine-diglycol)-benzene (PDB), a hybrid hydrogel is formed by the combination of PDB self-assembly with Ca2+ mediated alginate crosslinking.
28741860	0	18	theme	Sensing	12:18	arg1	Microarrays					20:30	Autoinducer Sensing Microarrays	0:30	Autoinducer Sensing Microarrays by Reporter Bacteria	0:51	Autoinducer Sensing Microarrays by Reporter Bacteria Encapsulated in Hybrid Supramolecular-Polysaccharide Hydrogels.
28741860	3	19	theme	autoinducers	766:777	arg1	diffusion					753:761	diffusion	753:761	diffusion of autoinducers into the gels	753:791	The different assembly mechanisms are shown not to interfere with each other and despite the more than four-fold increased moduli of the hydrogels, diffusion of autoinducers into the gels remains efficient and Escherichia coli pLuxR-green fluorescent protein (GFP) reporter bacteria are proliferating.
28741860	4	20	theme	reporter	926:933	arg1	hydrogels					951:959	reporter bacteria-loaded hydrogels	926:959	reporter bacteria-loaded hydrogels	926:959	Templating affords reporter bacteria-loaded hydrogels with controllable shape and size.
28741860	2	21	theme	PDB	545:547	arg1	self-assembly					549:561	PDB self-assembly	545:561	PDB self-assembly	545:561	For reinforcing hydrogels of 1,4-bi(phenylalanine-diglycol)-benzene (PDB), a hybrid hydrogel is formed by the combination of PDB self-assembly with Ca2+ mediated alginate crosslinking.
28741860	2	22	theme	1,4-bi	449:454	arg1	-benzene					479:486	1,4-bi(phenylalanine-diglycol)-benzene	449:486	1,4-bi(phenylalanine-diglycol)-benzene (PDB)	449:492	For reinforcing hydrogels of 1,4-bi(phenylalanine-diglycol)-benzene (PDB), a hybrid hydrogel is formed by the combination of PDB self-assembly with Ca2+ mediated alginate crosslinking.
28741860	2	22	theme	1,4-bi	449:454	arg1	PDB					489:491	PDB	489:491	PDB	489:491	For reinforcing hydrogels of 1,4-bi(phenylalanine-diglycol)-benzene (PDB), a hybrid hydrogel is formed by the combination of PDB self-assembly with Ca2+ mediated alginate crosslinking.
28741860	0	23	theme	Autoinducer	0:10	arg1	Microarrays					20:30	Autoinducer Sensing Microarrays	0:30	Autoinducer Sensing Microarrays by Reporter Bacteria	0:51	Autoinducer Sensing Microarrays by Reporter Bacteria Encapsulated in Hybrid Supramolecular-Polysaccharide Hydrogels.
28741860	1	24	theme	homoserine	336:345	arg1	lactone					347:353	N-acyl homoserine lactone	329:353	N-acyl homoserine lactone autoinducers (3OC12 HSL)	329:378	A generally applicable strategy to obtain mechanically robust hydrogels for the incorporation and containment of functional reporter bacteria for the microarray and microparticle-based detection and signaling of N-acyl homoserine lactone autoinducers (3OC12 HSL) at relevant concentrations is reported.
28741860	3	25	theme	efficient	801:809	arg1	bacteria					879:886	efficient and Escherichia coli pLuxR-green fluorescent protein (GFP) reporter bacteria	801:886	efficient and Escherichia coli pLuxR-green fluorescent protein (GFP) reporter bacteria	801:886	The different assembly mechanisms are shown not to interfere with each other and despite the more than four-fold increased moduli of the hydrogels, diffusion of autoinducers into the gels remains efficient and Escherichia coli pLuxR-green fluorescent protein (GFP) reporter bacteria are proliferating.
28741860	1	26	dep	incorporation	197:209	arg1	the					193:195	the	193:195	the	193:195	A generally applicable strategy to obtain mechanically robust hydrogels for the incorporation and containment of functional reporter bacteria for the microarray and microparticle-based detection and signaling of N-acyl homoserine lactone autoinducers (3OC12 HSL) at relevant concentrations is reported.
28741860	1	27	theme	functional	230:239	arg1	bacteria					250:257	functional reporter bacteria	230:257	functional reporter bacteria	230:257	A generally applicable strategy to obtain mechanically robust hydrogels for the incorporation and containment of functional reporter bacteria for the microarray and microparticle-based detection and signaling of N-acyl homoserine lactone autoinducers (3OC12 HSL) at relevant concentrations is reported.
28741860	1	28	theme	lactone	347:353	arg1	autoinducers					355:366	N-acyl homoserine lactone autoinducers	329:366	N-acyl homoserine lactone autoinducers (3OC12 HSL)	329:378	A generally applicable strategy to obtain mechanically robust hydrogels for the incorporation and containment of functional reporter bacteria for the microarray and microparticle-based detection and signaling of N-acyl homoserine lactone autoinducers (3OC12 HSL) at relevant concentrations is reported.
28741860	1	28	theme	lactone	347:353	arg1	HSL					375:377	3OC12 HSL	369:377	3OC12 HSL	369:377	A generally applicable strategy to obtain mechanically robust hydrogels for the incorporation and containment of functional reporter bacteria for the microarray and microparticle-based detection and signaling of N-acyl homoserine lactone autoinducers (3OC12 HSL) at relevant concentrations is reported.
28741860	1	29	theme	applicable	129:138	arg1	strategy					140:147	A generally applicable strategy	117:147	A generally applicable strategy to obtain mechanically robust hydrogels for the incorporation and containment of functional reporter bacteria for the microarray and microparticle-based detection and signaling of N-acyl homoserine lactone autoinducers (3OC12 HSL) at relevant concentrations	117:405	A generally applicable strategy to obtain mechanically robust hydrogels for the incorporation and containment of functional reporter bacteria for the microarray and microparticle-based detection and signaling of N-acyl homoserine lactone autoinducers (3OC12 HSL) at relevant concentrations is reported.
28741860	1	30	theme	reporter	241:248	arg1	bacteria					250:257	functional reporter bacteria	230:257	functional reporter bacteria	230:257	A generally applicable strategy to obtain mechanically robust hydrogels for the incorporation and containment of functional reporter bacteria for the microarray and microparticle-based detection and signaling of N-acyl homoserine lactone autoinducers (3OC12 HSL) at relevant concentrations is reported.
28741860	1	31	theme	autoinducers	355:366	arg1	signaling					316:324	signaling	316:324	signaling	316:324	A generally applicable strategy to obtain mechanically robust hydrogels for the incorporation and containment of functional reporter bacteria for the microarray and microparticle-based detection and signaling of N-acyl homoserine lactone autoinducers (3OC12 HSL) at relevant concentrations is reported.
28741860	1	31	theme	autoinducers	355:366	arg1	detection					302:310	the microarray and microparticle-based detection	263:310	detection	302:310	A generally applicable strategy to obtain mechanically robust hydrogels for the incorporation and containment of functional reporter bacteria for the microarray and microparticle-based detection and signaling of N-acyl homoserine lactone autoinducers (3OC12 HSL) at relevant concentrations is reported.
28741860	5	32	theme	GFP	1139:1141	arg1	expression					1143:1152	autoinducer-triggered GFP expression	1117:1152	autoinducer-triggered GFP expression	1117:1152	Upon exposure to 3OC12 HSL, the embedded bacteria exhibit an up to 12 ± 3 times increase in fluorescence intensity due to autoinducer-triggered GFP expression.
28741860	3	33	theme	assembly	619:626	arg1	mechanisms					628:637	The different assembly mechanisms	605:637	The different assembly mechanisms	605:637	The different assembly mechanisms are shown not to interfere with each other and despite the more than four-fold increased moduli of the hydrogels, diffusion of autoinducers into the gels remains efficient and Escherichia coli pLuxR-green fluorescent protein (GFP) reporter bacteria are proliferating.
28741860	1	34	theme	bacteria	250:257	arg1	containment					215:225	containment	215:225	containment	215:225	A generally applicable strategy to obtain mechanically robust hydrogels for the incorporation and containment of functional reporter bacteria for the microarray and microparticle-based detection and signaling of N-acyl homoserine lactone autoinducers (3OC12 HSL) at relevant concentrations is reported.
28741860	1	34	theme	bacteria	250:257	arg1	incorporation					197:209	incorporation	197:209	incorporation	197:209	A generally applicable strategy to obtain mechanically robust hydrogels for the incorporation and containment of functional reporter bacteria for the microarray and microparticle-based detection and signaling of N-acyl homoserine lactone autoinducers (3OC12 HSL) at relevant concentrations is reported.
28741860	5	35	from	increase	1075:1082	arg1	intensity					1100:1108	fluorescence intensity	1087:1108	fluorescence intensity	1087:1108	Upon exposure to 3OC12 HSL, the embedded bacteria exhibit an up to 12 ± 3 times increase in fluorescence intensity due to autoinducer-triggered GFP expression.
28741860	2	36	theme	-benzene	479:486	arg1	hydrogels					436:444	reinforcing hydrogels	424:444	reinforcing hydrogels of 1,4-bi(phenylalanine-diglycol)-benzene (PDB)	424:492	For reinforcing hydrogels of 1,4-bi(phenylalanine-diglycol)-benzene (PDB), a hybrid hydrogel is formed by the combination of PDB self-assembly with Ca2+ mediated alginate crosslinking.
28741860	0	37	theme	Reporter	35:42	arg1	Bacteria					44:51	Reporter Bacteria	35:51	Reporter Bacteria	35:51	Autoinducer Sensing Microarrays by Reporter Bacteria Encapsulated in Hybrid Supramolecular-Polysaccharide Hydrogels.
28741860	3	38	theme	pLuxR-green	832:842	arg1	GFP					865:867	GFP	865:867	GFP	865:867	The different assembly mechanisms are shown not to interfere with each other and despite the more than four-fold increased moduli of the hydrogels, diffusion of autoinducers into the gels remains efficient and Escherichia coli pLuxR-green fluorescent protein (GFP) reporter bacteria are proliferating.
28741860	3	38	theme	pLuxR-green	832:842	arg1	protein					856:862	pLuxR-green fluorescent protein	832:862	efficient and Escherichia coli pLuxR-green fluorescent protein (GFP) reporter bacteria	801:886	The different assembly mechanisms are shown not to interfere with each other and despite the more than four-fold increased moduli of the hydrogels, diffusion of autoinducers into the gels remains efficient and Escherichia coli pLuxR-green fluorescent protein (GFP) reporter bacteria are proliferating.
28741860	2	39	theme	alginate	582:589	arg1	crosslinking					591:602	Ca2+ mediated alginate crosslinking	568:602	Ca2+ mediated alginate crosslinking	568:602	For reinforcing hydrogels of 1,4-bi(phenylalanine-diglycol)-benzene (PDB), a hybrid hydrogel is formed by the combination of PDB self-assembly with Ca2+ mediated alginate crosslinking.
28741860	3	40	theme	protein	856:862	arg1	bacteria					879:886	efficient and Escherichia coli pLuxR-green fluorescent protein (GFP) reporter bacteria	801:886	efficient and Escherichia coli pLuxR-green fluorescent protein (GFP) reporter bacteria	801:886	The different assembly mechanisms are shown not to interfere with each other and despite the more than four-fold increased moduli of the hydrogels, diffusion of autoinducers into the gels remains efficient and Escherichia coli pLuxR-green fluorescent protein (GFP) reporter bacteria are proliferating.
28741860	5	41	dep	12	1062:1063	arg1	to					1059:1060	to	1059:1060	to	1059:1060	Upon exposure to 3OC12 HSL, the embedded bacteria exhibit an up to 12 ± 3 times increase in fluorescence intensity due to autoinducer-triggered GFP expression.
28741860	3	42	theme	fluorescent	844:854	arg1	GFP					865:867	GFP	865:867	GFP	865:867	The different assembly mechanisms are shown not to interfere with each other and despite the more than four-fold increased moduli of the hydrogels, diffusion of autoinducers into the gels remains efficient and Escherichia coli pLuxR-green fluorescent protein (GFP) reporter bacteria are proliferating.
28741860	3	42	theme	fluorescent	844:854	arg1	protein					856:862	pLuxR-green fluorescent protein	832:862	efficient and Escherichia coli pLuxR-green fluorescent protein (GFP) reporter bacteria	801:886	The different assembly mechanisms are shown not to interfere with each other and despite the more than four-fold increased moduli of the hydrogels, diffusion of autoinducers into the gels remains efficient and Escherichia coli pLuxR-green fluorescent protein (GFP) reporter bacteria are proliferating.
28741860	6	43	theme	applicable	1206:1215	arg1	strategy					1217:1224	a potentially generally applicable strategy	1182:1224	a potentially generally applicable strategy to sensitively detect bacteria via their secreted autoinducers	1182:1287	This approach can serve as a potentially generally applicable strategy to sensitively detect bacteria via their secreted autoinducers.
28741860	6	43	theme	applicable	1206:1215	arg1	approach					1160:1167	This approach	1155:1167	This approach	1155:1167	This approach can serve as a potentially generally applicable strategy to sensitively detect bacteria via their secreted autoinducers.
28741860	2	44	theme	reinforcing	424:434	arg1	hydrogels					436:444	reinforcing hydrogels	424:444	reinforcing hydrogels of 1,4-bi(phenylalanine-diglycol)-benzene (PDB)	424:492	For reinforcing hydrogels of 1,4-bi(phenylalanine-diglycol)-benzene (PDB), a hybrid hydrogel is formed by the combination of PDB self-assembly with Ca2+ mediated alginate crosslinking.
28741860	3	45	theme	Escherichia	815:825	arg1	bacteria					879:886	efficient and Escherichia coli pLuxR-green fluorescent protein (GFP) reporter bacteria	801:886	efficient and Escherichia coli pLuxR-green fluorescent protein (GFP) reporter bacteria	801:886	The different assembly mechanisms are shown not to interfere with each other and despite the more than four-fold increased moduli of the hydrogels, diffusion of autoinducers into the gels remains efficient and Escherichia coli pLuxR-green fluorescent protein (GFP) reporter bacteria are proliferating.
28741860	5	46	theme	fluorescence	1087:1098	arg1	intensity					1100:1108	fluorescence intensity	1087:1108	fluorescence intensity	1087:1108	Upon exposure to 3OC12 HSL, the embedded bacteria exhibit an up to 12 ± 3 times increase in fluorescence intensity due to autoinducer-triggered GFP expression.
28741860	1	47	theme	mechanically	159:170	arg1	hydrogels					179:187	mechanically robust hydrogels	159:187	mechanically robust hydrogels for the incorporation and containment of functional reporter bacteria for the microarray and microparticle-based detection and signaling of N-acyl homoserine lactone autoinducers (3OC12 HSL)	159:378	A generally applicable strategy to obtain mechanically robust hydrogels for the incorporation and containment of functional reporter bacteria for the microarray and microparticle-based detection and signaling of N-acyl homoserine lactone autoinducers (3OC12 HSL) at relevant concentrations is reported.
28741860	3	48	theme	different	609:617	arg1	mechanisms					628:637	The different assembly mechanisms	605:637	The different assembly mechanisms	605:637	The different assembly mechanisms are shown not to interfere with each other and despite the more than four-fold increased moduli of the hydrogels, diffusion of autoinducers into the gels remains efficient and Escherichia coli pLuxR-green fluorescent protein (GFP) reporter bacteria are proliferating.
28741860	1	49	theme	microarray	267:276	arg1	detection					302:310	the microarray and microparticle-based detection	263:310	detection	302:310	A generally applicable strategy to obtain mechanically robust hydrogels for the incorporation and containment of functional reporter bacteria for the microarray and microparticle-based detection and signaling of N-acyl homoserine lactone autoinducers (3OC12 HSL) at relevant concentrations is reported.
28741860	3	50	theme	four-fold	708:716	arg1	moduli					728:733	the more than four-fold increased moduli	694:733	the more than four-fold increased moduli of the hydrogels	694:750	The different assembly mechanisms are shown not to interfere with each other and despite the more than four-fold increased moduli of the hydrogels, diffusion of autoinducers into the gels remains efficient and Escherichia coli pLuxR-green fluorescent protein (GFP) reporter bacteria are proliferating.
28741860	1	51	theme	robust	172:177	arg1	hydrogels					179:187	mechanically robust hydrogels	159:187	mechanically robust hydrogels for the incorporation and containment of functional reporter bacteria for the microarray and microparticle-based detection and signaling of N-acyl homoserine lactone autoinducers (3OC12 HSL)	159:378	A generally applicable strategy to obtain mechanically robust hydrogels for the incorporation and containment of functional reporter bacteria for the microarray and microparticle-based detection and signaling of N-acyl homoserine lactone autoinducers (3OC12 HSL) at relevant concentrations is reported.
28741860	0	52	theme	Supramolecular-Polysaccharide	76:104	arg1	Hydrogels					106:114	Hybrid Supramolecular-Polysaccharide Hydrogels	69:114	Hybrid Supramolecular-Polysaccharide Hydrogels	69:114	Autoinducer Sensing Microarrays by Reporter Bacteria Encapsulated in Hybrid Supramolecular-Polysaccharide Hydrogels.
28741860	3	53	theme	increased	718:726	arg1	moduli					728:733	the more than four-fold increased moduli	694:733	the more than four-fold increased moduli of the hydrogels	694:750	The different assembly mechanisms are shown not to interfere with each other and despite the more than four-fold increased moduli of the hydrogels, diffusion of autoinducers into the gels remains efficient and Escherichia coli pLuxR-green fluorescent protein (GFP) reporter bacteria are proliferating.
28741860	1	54	theme	microparticle-based	282:300	arg1	detection					302:310	the microarray and microparticle-based detection	263:310	detection	302:310	A generally applicable strategy to obtain mechanically robust hydrogels for the incorporation and containment of functional reporter bacteria for the microarray and microparticle-based detection and signaling of N-acyl homoserine lactone autoinducers (3OC12 HSL) at relevant concentrations is reported.
28741860	0	55	theme	Hybrid	69:74	arg1	Hydrogels					106:114	Hybrid Supramolecular-Polysaccharide Hydrogels	69:114	Hybrid Supramolecular-Polysaccharide Hydrogels	69:114	Autoinducer Sensing Microarrays by Reporter Bacteria Encapsulated in Hybrid Supramolecular-Polysaccharide Hydrogels.
28741860	1	56	theme	relevant	383:390	arg1	concentrations					392:405	relevant concentrations	383:405	relevant concentrations	383:405	A generally applicable strategy to obtain mechanically robust hydrogels for the incorporation and containment of functional reporter bacteria for the microarray and microparticle-based detection and signaling of N-acyl homoserine lactone autoinducers (3OC12 HSL) at relevant concentrations is reported.
28741860	5	57	theme	3	1067:1067	arg1	times					1069:1073	times	1069:1073	times	1069:1073	Upon exposure to 3OC12 HSL, the embedded bacteria exhibit an up to 12 ± 3 times increase in fluorescence intensity due to autoinducer-triggered GFP expression.
28741860	1	58	dep	strategy	140:147	arg1	obtain					152:157	obtain	152:157	to obtain mechanically robust hydrogels for the incorporation and containment of functional reporter bacteria for the microarray and microparticle-based detection and signaling of N-acyl homoserine lactone autoinducers (3OC12 HSL) at relevant concentrations	149:405	A generally applicable strategy to obtain mechanically robust hydrogels for the incorporation and containment of functional reporter bacteria for the microarray and microparticle-based detection and signaling of N-acyl homoserine lactone autoinducers (3OC12 HSL) at relevant concentrations is reported.
27118501	0	0	theme	cores	62:66	arg1	properties					48:57	the properties	44:57	the properties of cores intended for the preparation of pellets with controlled glucose release	44:138	[Influence of the mesh of extrusion dies on the properties of cores intended for the preparation of pellets with controlled glucose release].
27118501	5	1	theme	active	711:716	arg1	agents					718:723	the following osmotically or swellable active agents	672:723	the following osmotically or swellable active agents	672:723	The cores contain 75-80% of glucose combined with one of the following osmotically or swellable active agents: croscarmellose sodium (ADS), a mixture of microcrystalline cellulose and carmellose sodium Avicel RC 591 (RC), polyethylene glycol 6000 (PEG), and carboxymethyl starch (CMS).
27118501	5	2	dep	contain	625:631	arg1	sodium					741:746	croscarmellose sodium	726:746	croscarmellose sodium (ADS)	726:752	The cores contain 75-80% of glucose combined with one of the following osmotically or swellable active agents: croscarmellose sodium (ADS), a mixture of microcrystalline cellulose and carmellose sodium Avicel RC 591 (RC), polyethylene glycol 6000 (PEG), and carboxymethyl starch (CMS).
27118501	5	2	dep	contain	625:631	arg1	mixture					757:763	a mixture	755:763	a mixture of microcrystalline cellulose and carmellose sodium Avicel RC 591 (RC)	755:834	The cores contain 75-80% of glucose combined with one of the following osmotically or swellable active agents: croscarmellose sodium (ADS), a mixture of microcrystalline cellulose and carmellose sodium Avicel RC 591 (RC), polyethylene glycol 6000 (PEG), and carboxymethyl starch (CMS).
27118501	5	2	dep	contain	625:631	arg1	glycol					850:855	polyethylene glycol 6000	837:860	polyethylene glycol 6000 (PEG)	837:866	The cores contain 75-80% of glucose combined with one of the following osmotically or swellable active agents: croscarmellose sodium (ADS), a mixture of microcrystalline cellulose and carmellose sodium Avicel RC 591 (RC), polyethylene glycol 6000 (PEG), and carboxymethyl starch (CMS).
27118501	5	2	dep	contain	625:631	arg1	ADS					749:751	ADS	749:751	ADS	749:751	The cores contain 75-80% of glucose combined with one of the following osmotically or swellable active agents: croscarmellose sodium (ADS), a mixture of microcrystalline cellulose and carmellose sodium Avicel RC 591 (RC), polyethylene glycol 6000 (PEG), and carboxymethyl starch (CMS).
27118501	5	2	dep	contain	625:631	arg1	starch					887:892	carboxymethyl starch	873:892	carboxymethyl starch (CMS)	873:898	The cores contain 75-80% of glucose combined with one of the following osmotically or swellable active agents: croscarmellose sodium (ADS), a mixture of microcrystalline cellulose and carmellose sodium Avicel RC 591 (RC), polyethylene glycol 6000 (PEG), and carboxymethyl starch (CMS).
27118501	5	2	dep	contain	625:631	arg1	PEG					863:865	PEG	863:865	PEG	863:865	The cores contain 75-80% of glucose combined with one of the following osmotically or swellable active agents: croscarmellose sodium (ADS), a mixture of microcrystalline cellulose and carmellose sodium Avicel RC 591 (RC), polyethylene glycol 6000 (PEG), and carboxymethyl starch (CMS).
27118501	5	2	dep	contain	625:631	arg1	CMS					895:897	CMS	895:897	CMS	895:897	The cores contain 75-80% of glucose combined with one of the following osmotically or swellable active agents: croscarmellose sodium (ADS), a mixture of microcrystalline cellulose and carmellose sodium Avicel RC 591 (RC), polyethylene glycol 6000 (PEG), and carboxymethyl starch (CMS).
27118501	10	3	theme	1.0	1628:1630	arg1	mm					1632:1633	mm	1632:1633	mm	1632:1633	Although the same compositions and manufacturing methods were used for all the pellets sizes, the cores produced through a 0.6 and 0.8 mm mesh screens showed similar properties while the properties of cores produced through a 1.0 mm mesh screen were rather different.
27118501	9	4	theme	physical	1313:1320	arg1	characteristics					1322:1336	The physical characteristics	1309:1336	The physical characteristics of the individual pellet sizes	1309:1367	The physical characteristics of the individual pellet sizes were different among themselves.
27118501	9	4	theme	physical	1313:1320	arg1	different					1374:1382	different	1374:1382	different	1374:1382	The physical characteristics of the individual pellet sizes were different among themselves.
27118501	5	5	theme	agents	718:723	arg1	agents					718:723	the following osmotically or swellable active agents	672:723	the following osmotically or swellable active agents	672:723	The cores contain 75-80% of glucose combined with one of the following osmotically or swellable active agents: croscarmellose sodium (ADS), a mixture of microcrystalline cellulose and carmellose sodium Avicel RC 591 (RC), polyethylene glycol 6000 (PEG), and carboxymethyl starch (CMS).
27118501	5	5	theme	agents	718:723	arg1	one					665:667	one	665:667	one	665:667	The cores contain 75-80% of glucose combined with one of the following osmotically or swellable active agents: croscarmellose sodium (ADS), a mixture of microcrystalline cellulose and carmellose sodium Avicel RC 591 (RC), polyethylene glycol 6000 (PEG), and carboxymethyl starch (CMS).
27118501	7	6	theme	pellets	1142:1148	arg1	evaluation					1124:1133	the physical evaluation	1111:1133	the physical evaluation of the pellets which were prepared and evaluated in previously published papers	1111:1213	The aim of the work was to statistically evaluate the results of the physical evaluation of the pellets which were prepared and evaluated in previously published papers.
27118501	10	7	theme	pellets	1481:1487	arg1	sizes					1489:1493	all the pellets sizes	1473:1493	all the pellets sizes	1473:1493	Although the same compositions and manufacturing methods were used for all the pellets sizes, the cores produced through a 0.6 and 0.8 mm mesh screens showed similar properties while the properties of cores produced through a 1.0 mm mesh screen were rather different.
27118501	10	8	theme	cores	1603:1607	arg1	different					1659:1667	different	1659:1667	different	1659:1667	Although the same compositions and manufacturing methods were used for all the pellets sizes, the cores produced through a 0.6 and 0.8 mm mesh screens showed similar properties while the properties of cores produced through a 1.0 mm mesh screen were rather different.
27118501	10	8	theme	cores	1603:1607	arg1	properties					1589:1598	the properties	1585:1598	the properties of cores produced through a 1.0 mm mesh screen	1585:1645	Although the same compositions and manufacturing methods were used for all the pellets sizes, the cores produced through a 0.6 and 0.8 mm mesh screens showed similar properties while the properties of cores produced through a 1.0 mm mesh screen were rather different.
27118501	3	9	theme	Hypoglycaemic	348:360	arg1	episodes					362:369	Hypoglycaemic episodes	348:369	Hypoglycaemic episodes	348:369	Hypoglycaemic episodes can be prevented by a dosage form with controlled release of glucose.
27118501	4	10	theme	sizes	499:503	arg1	cores					452:456	The pellet cores	441:456	The pellet cores of four compositions and three different sizes corresponding to the diameter of extrusion screen mesh (0.6, 0.8, and 1.0 mm)	441:581	The pellet cores of four compositions and three different sizes corresponding to the diameter of extrusion screen mesh (0.6, 0.8, and 1.0 mm) were prepared for this purpose.
27118501	10	11	theme	0.6	1525:1527	arg1	mm					1537:1538	mm	1537:1538	mm	1537:1538	Although the same compositions and manufacturing methods were used for all the pellets sizes, the cores produced through a 0.6 and 0.8 mm mesh screens showed similar properties while the properties of cores produced through a 1.0 mm mesh screen were rather different.
27118501	10	12	theme	mm	1632:1633	arg1	screen					1640:1645	a 1.0 mm mesh screen	1626:1645	a 1.0 mm mesh screen	1626:1645	Although the same compositions and manufacturing methods were used for all the pellets sizes, the cores produced through a 0.6 and 0.8 mm mesh screens showed similar properties while the properties of cores produced through a 1.0 mm mesh screen were rather different.
27118501	10	13	theme	same	1415:1418	arg1	compositions					1420:1431	the same compositions	1411:1431	the same compositions	1411:1431	Although the same compositions and manufacturing methods were used for all the pellets sizes, the cores produced through a 0.6 and 0.8 mm mesh screens showed similar properties while the properties of cores produced through a 1.0 mm mesh screen were rather different.
27118501	11	14	theme	data	1767:1770	arg1	meta-analysis					1750:1762	release glucose principal component analysis PCA meta-analysis	1701:1762	release glucose principal component analysis PCA meta-analysis of data	1701:1770	KEY WORDS hypoglycemia delayed release glucose principal component analysis PCA meta-analysis of data.
27118501	5	15	theme	following	676:684	arg1	agents					718:723	the following osmotically or swellable active agents	672:723	the following osmotically or swellable active agents	672:723	The cores contain 75-80% of glucose combined with one of the following osmotically or swellable active agents: croscarmellose sodium (ADS), a mixture of microcrystalline cellulose and carmellose sodium Avicel RC 591 (RC), polyethylene glycol 6000 (PEG), and carboxymethyl starch (CMS).
27118501	11	16	theme	release	1701:1707	arg1	meta-analysis					1750:1762	release glucose principal component analysis PCA meta-analysis	1701:1762	release glucose principal component analysis PCA meta-analysis of data	1701:1770	KEY WORDS hypoglycemia delayed release glucose principal component analysis PCA meta-analysis of data.
27118501	10	17	used	used	1464:1467	arg2	methods					1451:1457	manufacturing methods	1437:1457	manufacturing methods	1437:1457	Although the same compositions and manufacturing methods were used for all the pellets sizes, the cores produced through a 0.6 and 0.8 mm mesh screens showed similar properties while the properties of cores produced through a 1.0 mm mesh screen were rather different.
27118501	10	17	used	used	1464:1467	arg2	compositions					1420:1431	the same compositions	1411:1431	the same compositions	1411:1431	Although the same compositions and manufacturing methods were used for all the pellets sizes, the cores produced through a 0.6 and 0.8 mm mesh screens showed similar properties while the properties of cores produced through a 1.0 mm mesh screen were rather different.
27118501	3	18	with	form	400:403	arg1	release					421:427	controlled release	410:427	controlled release of glucose	410:438	Hypoglycaemic episodes can be prevented by a dosage form with controlled release of glucose.
27118501	5	19	theme	sodium	810:815	arg1	RC					824:825	carmellose sodium Avicel RC 591	799:829	carmellose sodium Avicel RC 591 (RC)	799:834	The cores contain 75-80% of glucose combined with one of the following osmotically or swellable active agents: croscarmellose sodium (ADS), a mixture of microcrystalline cellulose and carmellose sodium Avicel RC 591 (RC), polyethylene glycol 6000 (PEG), and carboxymethyl starch (CMS).
27118501	5	19	theme	sodium	810:815	arg1	RC					832:833	RC	832:833	RC	832:833	The cores contain 75-80% of glucose combined with one of the following osmotically or swellable active agents: croscarmellose sodium (ADS), a mixture of microcrystalline cellulose and carmellose sodium Avicel RC 591 (RC), polyethylene glycol 6000 (PEG), and carboxymethyl starch (CMS).
27118501	1	20	theme	UNLABELLED	142:151	arg1	mellitus					162:169	UNLABELLED Diabetes mellitus	142:169	UNLABELLED Diabetes mellitus	142:169	UNLABELLED Diabetes mellitus and its compensation are accompanied by serious complications.
27118501	5	21	theme	osmotically	686:696	arg1	agents					718:723	the following osmotically or swellable active agents	672:723	the following osmotically or swellable active agents	672:723	The cores contain 75-80% of glucose combined with one of the following osmotically or swellable active agents: croscarmellose sodium (ADS), a mixture of microcrystalline cellulose and carmellose sodium Avicel RC 591 (RC), polyethylene glycol 6000 (PEG), and carboxymethyl starch (CMS).
27118501	10	22	theme	mm	1537:1538	arg1	screens					1545:1551	a 0.6 and 0.8 mm mesh screens	1523:1551	a 0.6 and 0.8 mm mesh screens	1523:1551	Although the same compositions and manufacturing methods were used for all the pellets sizes, the cores produced through a 0.6 and 0.8 mm mesh screens showed similar properties while the properties of cores produced through a 1.0 mm mesh screen were rather different.
27118501	1	23	theme	Diabetes	153:160	arg1	mellitus					162:169	UNLABELLED Diabetes mellitus	142:169	UNLABELLED Diabetes mellitus	142:169	UNLABELLED Diabetes mellitus and its compensation are accompanied by serious complications.
27118501	4	24	theme	compositions	466:477	arg1	cores					452:456	The pellet cores	441:456	The pellet cores of four compositions and three different sizes corresponding to the diameter of extrusion screen mesh (0.6, 0.8, and 1.0 mm)	441:581	The pellet cores of four compositions and three different sizes corresponding to the diameter of extrusion screen mesh (0.6, 0.8, and 1.0 mm) were prepared for this purpose.
27118501	7	25	theme	physical	1115:1122	arg1	evaluation					1124:1133	the physical evaluation	1111:1133	the physical evaluation of the pellets which were prepared and evaluated in previously published papers	1111:1213	The aim of the work was to statistically evaluate the results of the physical evaluation of the pellets which were prepared and evaluated in previously published papers.
27118501	11	26	theme	glucose	1709:1715	arg1	meta-analysis					1750:1762	release glucose principal component analysis PCA meta-analysis	1701:1762	release glucose principal component analysis PCA meta-analysis of data	1701:1770	KEY WORDS hypoglycemia delayed release glucose principal component analysis PCA meta-analysis of data.
27118501	8	27	theme	meta-analysis	1259:1271	arg1	form					1249:1252	the form	1245:1252	the form of a meta-analysis using principle component analysis	1245:1306	The results are processed in the form of a meta-analysis using principle component analysis.
27118501	11	28	theme	analysis	1737:1744	arg1	meta-analysis					1750:1762	release glucose principal component analysis PCA meta-analysis	1701:1762	release glucose principal component analysis PCA meta-analysis of data	1701:1770	KEY WORDS hypoglycemia delayed release glucose principal component analysis PCA meta-analysis of data.
27118501	3	29	theme	controlled	410:419	arg1	release					421:427	controlled release	410:427	controlled release of glucose	410:438	Hypoglycaemic episodes can be prevented by a dosage form with controlled release of glucose.
27118501	11	30	theme	WORDS	1674:1678	arg1	hypoglycemia					1680:1691	KEY WORDS hypoglycemia	1670:1691	KEY WORDS hypoglycemia	1670:1691	KEY WORDS hypoglycemia delayed release glucose principal component analysis PCA meta-analysis of data.
27118501	4	31	theme	screen	548:553	arg1	mm					579:580	0.6, 0.8, and 1.0 mm	561:580	0.6, 0.8, and 1.0 mm	561:580	The pellet cores of four compositions and three different sizes corresponding to the diameter of extrusion screen mesh (0.6, 0.8, and 1.0 mm) were prepared for this purpose.
27118501	4	31	theme	screen	548:553	arg1	mesh					555:558	extrusion screen mesh	538:558	extrusion screen mesh (0.6, 0.8, and 1.0 mm)	538:581	The pellet cores of four compositions and three different sizes corresponding to the diameter of extrusion screen mesh (0.6, 0.8, and 1.0 mm) were prepared for this purpose.
27118501	5	32	theme	carmellose	799:808	arg1	RC					824:825	carmellose sodium Avicel RC 591	799:829	carmellose sodium Avicel RC 591 (RC)	799:834	The cores contain 75-80% of glucose combined with one of the following osmotically or swellable active agents: croscarmellose sodium (ADS), a mixture of microcrystalline cellulose and carmellose sodium Avicel RC 591 (RC), polyethylene glycol 6000 (PEG), and carboxymethyl starch (CMS).
27118501	5	32	theme	carmellose	799:808	arg1	RC					832:833	RC	832:833	RC	832:833	The cores contain 75-80% of glucose combined with one of the following osmotically or swellable active agents: croscarmellose sodium (ADS), a mixture of microcrystalline cellulose and carmellose sodium Avicel RC 591 (RC), polyethylene glycol 6000 (PEG), and carboxymethyl starch (CMS).
27118501	5	33	theme	Avicel	817:822	arg1	RC					824:825	carmellose sodium Avicel RC 591	799:829	carmellose sodium Avicel RC 591 (RC)	799:834	The cores contain 75-80% of glucose combined with one of the following osmotically or swellable active agents: croscarmellose sodium (ADS), a mixture of microcrystalline cellulose and carmellose sodium Avicel RC 591 (RC), polyethylene glycol 6000 (PEG), and carboxymethyl starch (CMS).
27118501	5	33	theme	Avicel	817:822	arg1	RC					832:833	RC	832:833	RC	832:833	The cores contain 75-80% of glucose combined with one of the following osmotically or swellable active agents: croscarmellose sodium (ADS), a mixture of microcrystalline cellulose and carmellose sodium Avicel RC 591 (RC), polyethylene glycol 6000 (PEG), and carboxymethyl starch (CMS).
27118501	0	34	theme	mesh	18:21	arg1	[Influence					0:9	[Influence	0:9	[Influence of the mesh of extrusion	0:34	[Influence of the mesh of extrusion dies on the properties of cores intended for the preparation of pellets with controlled glucose release].
27118501	4	35	theme	extrusion	538:546	arg1	mm					579:580	0.6, 0.8, and 1.0 mm	561:580	0.6, 0.8, and 1.0 mm	561:580	The pellet cores of four compositions and three different sizes corresponding to the diameter of extrusion screen mesh (0.6, 0.8, and 1.0 mm) were prepared for this purpose.
27118501	4	35	theme	extrusion	538:546	arg1	mesh					555:558	extrusion screen mesh	538:558	extrusion screen mesh (0.6, 0.8, and 1.0 mm)	538:581	The pellet cores of four compositions and three different sizes corresponding to the diameter of extrusion screen mesh (0.6, 0.8, and 1.0 mm) were prepared for this purpose.
27118501	0	36	theme	pellets	100:106	arg1	preparation					85:95	the preparation	81:95	the preparation of pellets	81:106	[Influence of the mesh of extrusion dies on the properties of cores intended for the preparation of pellets with controlled glucose release].
27118501	9	37	theme	individual	1345:1354	arg1	sizes					1363:1367	the individual pellet sizes	1341:1367	the individual pellet sizes	1341:1367	The physical characteristics of the individual pellet sizes were different among themselves.
27118501	8	38	theme	principle	1279:1287	arg1	analysis					1299:1306	principle component analysis	1279:1306	principle component analysis	1279:1306	The results are processed in the form of a meta-analysis using principle component analysis.
27118501	6	39	theme	subsequent	974:983	arg1	coating					985:991	subsequent coating	974:991	subsequent coating	974:991	The cores were prepared by extrusion-spheronization and are intended for subsequent coating by a semipermeable membrane based on ethylcellulose.
27118501	5	40	theme	carboxymethyl	873:885	arg1	CMS					895:897	CMS	895:897	CMS	895:897	The cores contain 75-80% of glucose combined with one of the following osmotically or swellable active agents: croscarmellose sodium (ADS), a mixture of microcrystalline cellulose and carmellose sodium Avicel RC 591 (RC), polyethylene glycol 6000 (PEG), and carboxymethyl starch (CMS).
27118501	5	40	theme	carboxymethyl	873:885	arg1	starch					887:892	carboxymethyl starch	873:892	carboxymethyl starch (CMS)	873:898	The cores contain 75-80% of glucose combined with one of the following osmotically or swellable active agents: croscarmellose sodium (ADS), a mixture of microcrystalline cellulose and carmellose sodium Avicel RC 591 (RC), polyethylene glycol 6000 (PEG), and carboxymethyl starch (CMS).
27118501	10	41	theme	mesh	1635:1638	arg1	screen					1640:1645	a 1.0 mm mesh screen	1626:1645	a 1.0 mm mesh screen	1626:1645	Although the same compositions and manufacturing methods were used for all the pellets sizes, the cores produced through a 0.6 and 0.8 mm mesh screens showed similar properties while the properties of cores produced through a 1.0 mm mesh screen were rather different.
27118501	0	42	theme	extrusion	26:34	arg1	mesh					18:21	the mesh	14:21	the mesh of extrusion	14:34	[Influence of the mesh of extrusion dies on the properties of cores intended for the preparation of pellets with controlled glucose release].
27118501	4	43	theme	mesh	555:558	arg1	diameter					526:533	the diameter	522:533	the diameter of extrusion screen mesh (0.6, 0.8, and 1.0 mm)	522:581	The pellet cores of four compositions and three different sizes corresponding to the diameter of extrusion screen mesh (0.6, 0.8, and 1.0 mm) were prepared for this purpose.
27118501	0	44	theme	controlled	113:122	arg1	release					132:138	controlled glucose release	113:138	controlled glucose release	113:138	[Influence of the mesh of extrusion dies on the properties of cores intended for the preparation of pellets with controlled glucose release].
27118501	10	45	theme	mesh	1540:1543	arg1	screens					1545:1551	a 0.6 and 0.8 mm mesh screens	1523:1551	a 0.6 and 0.8 mm mesh screens	1523:1551	Although the same compositions and manufacturing methods were used for all the pellets sizes, the cores produced through a 0.6 and 0.8 mm mesh screens showed similar properties while the properties of cores produced through a 1.0 mm mesh screen were rather different.
27118501	5	46	theme	microcrystalline	768:783	arg1	cellulose					785:793	microcrystalline cellulose	768:793	microcrystalline cellulose	768:793	The cores contain 75-80% of glucose combined with one of the following osmotically or swellable active agents: croscarmellose sodium (ADS), a mixture of microcrystalline cellulose and carmellose sodium Avicel RC 591 (RC), polyethylene glycol 6000 (PEG), and carboxymethyl starch (CMS).
27118501	5	47	theme	croscarmellose	726:739	arg1	sodium					741:746	croscarmellose sodium	726:746	croscarmellose sodium (ADS)	726:752	The cores contain 75-80% of glucose combined with one of the following osmotically or swellable active agents: croscarmellose sodium (ADS), a mixture of microcrystalline cellulose and carmellose sodium Avicel RC 591 (RC), polyethylene glycol 6000 (PEG), and carboxymethyl starch (CMS).
27118501	5	47	theme	croscarmellose	726:739	arg1	ADS					749:751	ADS	749:751	ADS	749:751	The cores contain 75-80% of glucose combined with one of the following osmotically or swellable active agents: croscarmellose sodium (ADS), a mixture of microcrystalline cellulose and carmellose sodium Avicel RC 591 (RC), polyethylene glycol 6000 (PEG), and carboxymethyl starch (CMS).
27118501	11	48	theme	PCA	1746:1748	arg1	meta-analysis					1750:1762	release glucose principal component analysis PCA meta-analysis	1701:1762	release glucose principal component analysis PCA meta-analysis of data	1701:1770	KEY WORDS hypoglycemia delayed release glucose principal component analysis PCA meta-analysis of data.
27118501	10	49	theme	0.8	1533:1535	arg1	screens					1545:1551	a 0.6 and 0.8 mm mesh screens	1523:1551	a 0.6 and 0.8 mm mesh screens	1523:1551	Although the same compositions and manufacturing methods were used for all the pellets sizes, the cores produced through a 0.6 and 0.8 mm mesh screens showed similar properties while the properties of cores produced through a 1.0 mm mesh screen were rather different.
27118501	7	50	theme	work	1061:1064	arg1	aim					1050:1052	The aim	1046:1052	The aim of the work	1046:1064	The aim of the work was to statistically evaluate the results of the physical evaluation of the pellets which were prepared and evaluated in previously published papers.
27118501	5	51	theme	cellulose	785:793	arg1	sodium					741:746	croscarmellose sodium	726:746	croscarmellose sodium (ADS)	726:752	The cores contain 75-80% of glucose combined with one of the following osmotically or swellable active agents: croscarmellose sodium (ADS), a mixture of microcrystalline cellulose and carmellose sodium Avicel RC 591 (RC), polyethylene glycol 6000 (PEG), and carboxymethyl starch (CMS).
27118501	5	51	theme	cellulose	785:793	arg1	mixture					757:763	a mixture	755:763	a mixture of microcrystalline cellulose and carmellose sodium Avicel RC 591 (RC)	755:834	The cores contain 75-80% of glucose combined with one of the following osmotically or swellable active agents: croscarmellose sodium (ADS), a mixture of microcrystalline cellulose and carmellose sodium Avicel RC 591 (RC), polyethylene glycol 6000 (PEG), and carboxymethyl starch (CMS).
27118501	5	51	theme	cellulose	785:793	arg1	glycol					850:855	polyethylene glycol 6000	837:860	polyethylene glycol 6000 (PEG)	837:866	The cores contain 75-80% of glucose combined with one of the following osmotically or swellable active agents: croscarmellose sodium (ADS), a mixture of microcrystalline cellulose and carmellose sodium Avicel RC 591 (RC), polyethylene glycol 6000 (PEG), and carboxymethyl starch (CMS).
27118501	5	51	theme	cellulose	785:793	arg1	ADS					749:751	ADS	749:751	ADS	749:751	The cores contain 75-80% of glucose combined with one of the following osmotically or swellable active agents: croscarmellose sodium (ADS), a mixture of microcrystalline cellulose and carmellose sodium Avicel RC 591 (RC), polyethylene glycol 6000 (PEG), and carboxymethyl starch (CMS).
27118501	5	51	theme	cellulose	785:793	arg1	starch					887:892	carboxymethyl starch	873:892	carboxymethyl starch (CMS)	873:898	The cores contain 75-80% of glucose combined with one of the following osmotically or swellable active agents: croscarmellose sodium (ADS), a mixture of microcrystalline cellulose and carmellose sodium Avicel RC 591 (RC), polyethylene glycol 6000 (PEG), and carboxymethyl starch (CMS).
27118501	5	51	theme	cellulose	785:793	arg1	PEG					863:865	PEG	863:865	PEG	863:865	The cores contain 75-80% of glucose combined with one of the following osmotically or swellable active agents: croscarmellose sodium (ADS), a mixture of microcrystalline cellulose and carmellose sodium Avicel RC 591 (RC), polyethylene glycol 6000 (PEG), and carboxymethyl starch (CMS).
27118501	5	51	theme	cellulose	785:793	arg1	CMS					895:897	CMS	895:897	CMS	895:897	The cores contain 75-80% of glucose combined with one of the following osmotically or swellable active agents: croscarmellose sodium (ADS), a mixture of microcrystalline cellulose and carmellose sodium Avicel RC 591 (RC), polyethylene glycol 6000 (PEG), and carboxymethyl starch (CMS).
27118501	9	52	theme	pellet	1356:1361	arg1	sizes					1363:1367	the individual pellet sizes	1341:1367	the individual pellet sizes	1341:1367	The physical characteristics of the individual pellet sizes were different among themselves.
27118501	4	53	theme	pellet	445:450	arg1	cores					452:456	The pellet cores	441:456	The pellet cores of four compositions and three different sizes corresponding to the diameter of extrusion screen mesh (0.6, 0.8, and 1.0 mm)	441:581	The pellet cores of four compositions and three different sizes corresponding to the diameter of extrusion screen mesh (0.6, 0.8, and 1.0 mm) were prepared for this purpose.
27118501	2	54	theme	peroral	325:331	arg1	antidiabetics					333:345	certain peroral antidiabetics	317:345	certain peroral antidiabetics	317:345	One of them is hypoglycaemia, which occurs in patients treated with insulin and/or certain peroral antidiabetics.
27118501	3	55	theme	dosage	393:398	arg1	form					400:403	a dosage form	391:403	a dosage form with controlled release of glucose	391:438	Hypoglycaemic episodes can be prevented by a dosage form with controlled release of glucose.
27118501	11	56	theme	component	1727:1735	arg1	meta-analysis					1750:1762	release glucose principal component analysis PCA meta-analysis	1701:1762	release glucose principal component analysis PCA meta-analysis of data	1701:1770	KEY WORDS hypoglycemia delayed release glucose principal component analysis PCA meta-analysis of data.
27118501	0	57	theme	glucose	124:130	arg1	release					132:138	controlled glucose release	113:138	controlled glucose release	113:138	[Influence of the mesh of extrusion dies on the properties of cores intended for the preparation of pellets with controlled glucose release].
27118501	2	58	theme	certain	317:323	arg1	antidiabetics					333:345	certain peroral antidiabetics	317:345	certain peroral antidiabetics	317:345	One of them is hypoglycaemia, which occurs in patients treated with insulin and/or certain peroral antidiabetics.
27118501	5	59	contain	contain	625:631	arg2	%					638:638	75-80%	633:638	75-80% of glucose combined with one of the following osmotically or swellable active agents	633:723	The cores contain 75-80% of glucose combined with one of the following osmotically or swellable active agents: croscarmellose sodium (ADS), a mixture of microcrystalline cellulose and carmellose sodium Avicel RC 591 (RC), polyethylene glycol 6000 (PEG), and carboxymethyl starch (CMS).
27118501	5	59	contain	contain	625:631	arg2	glucose					643:649	glucose	643:649	glucose combined with one of the following osmotically or swellable active agents	643:723	The cores contain 75-80% of glucose combined with one of the following osmotically or swellable active agents: croscarmellose sodium (ADS), a mixture of microcrystalline cellulose and carmellose sodium Avicel RC 591 (RC), polyethylene glycol 6000 (PEG), and carboxymethyl starch (CMS).
27118501	5	59	contain	contain	625:631	arg1	cores					619:623	The cores	615:623	The cores	615:623	The cores contain 75-80% of glucose combined with one of the following osmotically or swellable active agents: croscarmellose sodium (ADS), a mixture of microcrystalline cellulose and carmellose sodium Avicel RC 591 (RC), polyethylene glycol 6000 (PEG), and carboxymethyl starch (CMS).
27118501	10	60	theme	manufacturing	1437:1449	arg1	methods					1451:1457	manufacturing methods	1437:1457	manufacturing methods	1437:1457	Although the same compositions and manufacturing methods were used for all the pellets sizes, the cores produced through a 0.6 and 0.8 mm mesh screens showed similar properties while the properties of cores produced through a 1.0 mm mesh screen were rather different.
27118501	8	61	theme	component	1289:1297	arg1	analysis					1299:1306	principle component analysis	1279:1306	principle component analysis	1279:1306	The results are processed in the form of a meta-analysis using principle component analysis.
27118501	5	62	theme	polyethylene	837:848	arg1	PEG					863:865	PEG	863:865	PEG	863:865	The cores contain 75-80% of glucose combined with one of the following osmotically or swellable active agents: croscarmellose sodium (ADS), a mixture of microcrystalline cellulose and carmellose sodium Avicel RC 591 (RC), polyethylene glycol 6000 (PEG), and carboxymethyl starch (CMS).
27118501	5	62	theme	polyethylene	837:848	arg1	glycol					850:855	polyethylene glycol 6000	837:860	polyethylene glycol 6000 (PEG)	837:866	The cores contain 75-80% of glucose combined with one of the following osmotically or swellable active agents: croscarmellose sodium (ADS), a mixture of microcrystalline cellulose and carmellose sodium Avicel RC 591 (RC), polyethylene glycol 6000 (PEG), and carboxymethyl starch (CMS).
27118501	11	63	theme	KEY	1670:1672	arg1	hypoglycemia					1680:1691	KEY WORDS hypoglycemia	1670:1691	KEY WORDS hypoglycemia	1670:1691	KEY WORDS hypoglycemia delayed release glucose principal component analysis PCA meta-analysis of data.
27118501	2	64	theme	them	241:244	arg1	them					241:244	them	241:244	them	241:244	One of them is hypoglycaemia, which occurs in patients treated with insulin and/or certain peroral antidiabetics.
27118501	2	64	theme	them	241:244	arg1	One					234:236	One	234:236	One	234:236	One of them is hypoglycaemia, which occurs in patients treated with insulin and/or certain peroral antidiabetics.
27118501	11	65	theme	principal	1717:1725	arg1	meta-analysis					1750:1762	release glucose principal component analysis PCA meta-analysis	1701:1762	release glucose principal component analysis PCA meta-analysis of data	1701:1770	KEY WORDS hypoglycemia delayed release glucose principal component analysis PCA meta-analysis of data.
27118501	9	66	theme	sizes	1363:1367	arg1	characteristics					1322:1336	The physical characteristics	1309:1336	The physical characteristics of the individual pellet sizes	1309:1367	The physical characteristics of the individual pellet sizes were different among themselves.
27118501	9	66	theme	sizes	1363:1367	arg1	different					1374:1382	different	1374:1382	different	1374:1382	The physical characteristics of the individual pellet sizes were different among themselves.
27118501	1	67	theme	serious	211:217	arg1	complications					219:231	serious complications	211:231	serious complications	211:231	UNLABELLED Diabetes mellitus and its compensation are accompanied by serious complications.
27118501	7	68	theme	evaluation	1124:1133	arg1	results					1100:1106	the results	1096:1106	the results of the physical evaluation of the pellets which were prepared and evaluated in previously published papers	1096:1213	The aim of the work was to statistically evaluate the results of the physical evaluation of the pellets which were prepared and evaluated in previously published papers.
27118501	7	69	theme	published	1198:1206	arg1	papers					1208:1213	previously published papers	1187:1213	previously published papers	1187:1213	The aim of the work was to statistically evaluate the results of the physical evaluation of the pellets which were prepared and evaluated in previously published papers.
27118501	5	70	theme	RC	824:825	arg1	sodium					741:746	croscarmellose sodium	726:746	croscarmellose sodium (ADS)	726:752	The cores contain 75-80% of glucose combined with one of the following osmotically or swellable active agents: croscarmellose sodium (ADS), a mixture of microcrystalline cellulose and carmellose sodium Avicel RC 591 (RC), polyethylene glycol 6000 (PEG), and carboxymethyl starch (CMS).
27118501	5	70	theme	RC	824:825	arg1	mixture					757:763	a mixture	755:763	a mixture of microcrystalline cellulose and carmellose sodium Avicel RC 591 (RC)	755:834	The cores contain 75-80% of glucose combined with one of the following osmotically or swellable active agents: croscarmellose sodium (ADS), a mixture of microcrystalline cellulose and carmellose sodium Avicel RC 591 (RC), polyethylene glycol 6000 (PEG), and carboxymethyl starch (CMS).
27118501	5	70	theme	RC	824:825	arg1	glycol					850:855	polyethylene glycol 6000	837:860	polyethylene glycol 6000 (PEG)	837:866	The cores contain 75-80% of glucose combined with one of the following osmotically or swellable active agents: croscarmellose sodium (ADS), a mixture of microcrystalline cellulose and carmellose sodium Avicel RC 591 (RC), polyethylene glycol 6000 (PEG), and carboxymethyl starch (CMS).
27118501	5	70	theme	RC	824:825	arg1	ADS					749:751	ADS	749:751	ADS	749:751	The cores contain 75-80% of glucose combined with one of the following osmotically or swellable active agents: croscarmellose sodium (ADS), a mixture of microcrystalline cellulose and carmellose sodium Avicel RC 591 (RC), polyethylene glycol 6000 (PEG), and carboxymethyl starch (CMS).
27118501	5	70	theme	RC	824:825	arg1	starch					887:892	carboxymethyl starch	873:892	carboxymethyl starch (CMS)	873:898	The cores contain 75-80% of glucose combined with one of the following osmotically or swellable active agents: croscarmellose sodium (ADS), a mixture of microcrystalline cellulose and carmellose sodium Avicel RC 591 (RC), polyethylene glycol 6000 (PEG), and carboxymethyl starch (CMS).
27118501	5	70	theme	RC	824:825	arg1	PEG					863:865	PEG	863:865	PEG	863:865	The cores contain 75-80% of glucose combined with one of the following osmotically or swellable active agents: croscarmellose sodium (ADS), a mixture of microcrystalline cellulose and carmellose sodium Avicel RC 591 (RC), polyethylene glycol 6000 (PEG), and carboxymethyl starch (CMS).
27118501	5	70	theme	RC	824:825	arg1	CMS					895:897	CMS	895:897	CMS	895:897	The cores contain 75-80% of glucose combined with one of the following osmotically or swellable active agents: croscarmellose sodium (ADS), a mixture of microcrystalline cellulose and carmellose sodium Avicel RC 591 (RC), polyethylene glycol 6000 (PEG), and carboxymethyl starch (CMS).
27118501	3	71	theme	glucose	432:438	arg1	release					421:427	controlled release	410:427	controlled release of glucose	410:438	Hypoglycaemic episodes can be prevented by a dosage form with controlled release of glucose.
27118501	10	72	theme	similar	1560:1566	arg1	properties					1568:1577	similar properties	1560:1577	similar properties	1560:1577	Although the same compositions and manufacturing methods were used for all the pellets sizes, the cores produced through a 0.6 and 0.8 mm mesh screens showed similar properties while the properties of cores produced through a 1.0 mm mesh screen were rather different.
27118501	5	73	theme	swellable	701:709	arg1	agents					718:723	the following osmotically or swellable active agents	672:723	the following osmotically or swellable active agents	672:723	The cores contain 75-80% of glucose combined with one of the following osmotically or swellable active agents: croscarmellose sodium (ADS), a mixture of microcrystalline cellulose and carmellose sodium Avicel RC 591 (RC), polyethylene glycol 6000 (PEG), and carboxymethyl starch (CMS).
27118501	4	74	theme	different	489:497	arg1	sizes					499:503	three different sizes	483:503	three different sizes	483:503	The pellet cores of four compositions and three different sizes corresponding to the diameter of extrusion screen mesh (0.6, 0.8, and 1.0 mm) were prepared for this purpose.
27118501	5	75	theme	glucose	643:649	arg1	%					638:638	75-80%	633:638	75-80% of glucose combined with one of the following osmotically or swellable active agents	633:723	The cores contain 75-80% of glucose combined with one of the following osmotically or swellable active agents: croscarmellose sodium (ADS), a mixture of microcrystalline cellulose and carmellose sodium Avicel RC 591 (RC), polyethylene glycol 6000 (PEG), and carboxymethyl starch (CMS).
27118501	5	75	theme	glucose	643:649	arg1	glucose					643:649	glucose	643:649	glucose combined with one of the following osmotically or swellable active agents	643:723	The cores contain 75-80% of glucose combined with one of the following osmotically or swellable active agents: croscarmellose sodium (ADS), a mixture of microcrystalline cellulose and carmellose sodium Avicel RC 591 (RC), polyethylene glycol 6000 (PEG), and carboxymethyl starch (CMS).
27118501	6	76	theme	semipermeable	998:1010	arg1	membrane					1012:1019	a semipermeable membrane	996:1019	a semipermeable membrane based on ethylcellulose	996:1043	The cores were prepared by extrusion-spheronization and are intended for subsequent coating by a semipermeable membrane based on ethylcellulose.
27071112	0	0	theme	polymerase	87:96	arg1	activity					98:105	cell wall polymerase activity	77:105	cell wall polymerase activity	77:105	Cofactor bypass variants reveal a conformational control mechanism governing cell wall polymerase activity.
27071112	3	1	theme	coli	481:484	arg1	synthases					440:448	the PG synthases PBP1b and PBP1a	433:464	the PG synthases PBP1b and PBP1a of Escherichia coli	433:484	We and others have shown that the PG synthases PBP1b and PBP1a of Escherichia coli require the outer membrane lipoproteins LpoA and LpoB, respectively, for their in vivo function.
27071112	3	1	theme	coli	481:484	arg1	PBP1a					460:464	PBP1a	460:464	PBP1a	460:464	We and others have shown that the PG synthases PBP1b and PBP1a of Escherichia coli require the outer membrane lipoproteins LpoA and LpoB, respectively, for their in vivo function.
27071112	3	1	theme	coli	481:484	arg1	PBP1b					450:454	PBP1b	450:454	PBP1b	450:454	We and others have shown that the PG synthases PBP1b and PBP1a of Escherichia coli require the outer membrane lipoproteins LpoA and LpoB, respectively, for their in vivo function.
27071112	8	2	theme	PBP1b-LpoB	1595:1604	arg1	complex					1606:1612	the PBP1b-LpoB complex	1591:1612	the PBP1b-LpoB complex	1591:1612	Finally, phenotypic analysis of strains carrying a PBP1b* variant revealed that the PBP1b-LpoB complex is most likely not providing an important physical link between the inner and outer membranes at the division site, as has been previously proposed.
27071112	7	3	theme	transferase	1486:1496	arg1	site					1505:1508	the glycosyl transferase active site	1473:1508	the glycosyl transferase active site	1473:1508	Moreover, the location of amino acid substitutions causing the bypass phenotype on the PBP1b structure support a model in which polymerization activation proceeds via the induction of a conformational change in PBP1b initiated by LpoB binding to its UB2H domain, followed by its transmission to the glycosyl transferase active site.
27071112	0	4	theme	wall	82:85	arg1	activity					98:105	cell wall polymerase activity	77:105	cell wall polymerase activity	77:105	Cofactor bypass variants reveal a conformational control mechanism governing cell wall polymerase activity.
27071112	7	5	theme	LpoB	1408:1411	arg1	binding					1413:1419	LpoB binding	1408:1419	LpoB binding to its UB2H domain	1408:1438	Moreover, the location of amino acid substitutions causing the bypass phenotype on the PBP1b structure support a model in which polymerization activation proceeds via the induction of a conformational change in PBP1b initiated by LpoB binding to its UB2H domain, followed by its transmission to the glycosyl transferase active site.
27071112	7	6	theme	UB2H	1428:1431	arg1	domain					1433:1438	its UB2H domain	1424:1438	its UB2H domain	1424:1438	Moreover, the location of amino acid substitutions causing the bypass phenotype on the PBP1b structure support a model in which polymerization activation proceeds via the induction of a conformational change in PBP1b initiated by LpoB binding to its UB2H domain, followed by its transmission to the glycosyl transferase active site.
27071112	1	7	theme	osmotic	166:172	arg1	rupture					174:180	osmotic rupture	166:180	osmotic rupture	166:180	To fortify their cytoplasmic membrane and protect it from osmotic rupture, most bacteria surround themselves with a peptidoglycan (PG) exoskeleton synthesized by the penicillin-binding proteins (PBPs).
27071112	7	8	theme	active	1498:1503	arg1	site					1505:1508	the glycosyl transferase active site	1473:1508	the glycosyl transferase active site	1473:1508	Moreover, the location of amino acid substitutions causing the bypass phenotype on the PBP1b structure support a model in which polymerization activation proceeds via the induction of a conformational change in PBP1b initiated by LpoB binding to its UB2H domain, followed by its transmission to the glycosyl transferase active site.
27071112	6	9	theme	relevant	1142:1149	arg1	functions					1151:1159	the relevant functions	1138:1159	the relevant functions of LpoB in vivo	1138:1175	Several of these PBP1b variants were isolated and displayed elevated polymerization activity in vitro, indicating that the activation of glycan polymer growth is indeed one of the relevant functions of LpoB in vivo.
27071112	6	10	theme	growth	1114:1119	arg1	activation					1085:1094	the activation	1081:1094	the activation of glycan polymer growth	1081:1119	Several of these PBP1b variants were isolated and displayed elevated polymerization activity in vitro, indicating that the activation of glycan polymer growth is indeed one of the relevant functions of LpoB in vivo.
27071112	6	10	theme	growth	1114:1119	arg1	functions					1151:1159	the relevant functions	1138:1159	the relevant functions of LpoB in vivo	1138:1175	Several of these PBP1b variants were isolated and displayed elevated polymerization activity in vitro, indicating that the activation of glycan polymer growth is indeed one of the relevant functions of LpoB in vivo.
27071112	6	10	theme	growth	1114:1119	arg1	one					1131:1133	one	1131:1133	one	1131:1133	Several of these PBP1b variants were isolated and displayed elevated polymerization activity in vitro, indicating that the activation of glycan polymer growth is indeed one of the relevant functions of LpoB in vivo.
27071112	4	11	theme	physiological	723:735	arg1	relevance					737:745	its physiological relevance	719:745	its physiological relevance	719:745	Although it has been demonstrated that LpoB activates the PG polymerization activity of PBP1b in vitro, the mechanism of activation and its physiological relevance have remained unclear.
27071112	7	12	from	change	1379:1384	arg1	PBP1b					1389:1393	PBP1b	1389:1393	PBP1b initiated by LpoB binding to its UB2H domain	1389:1438	Moreover, the location of amino acid substitutions causing the bypass phenotype on the PBP1b structure support a model in which polymerization activation proceeds via the induction of a conformational change in PBP1b initiated by LpoB binding to its UB2H domain, followed by its transmission to the glycosyl transferase active site.
27071112	8	13	theme	*	1567:1567	arg1	variant					1569:1575	a PBP1b* variant	1560:1575	a PBP1b* variant	1560:1575	Finally, phenotypic analysis of strains carrying a PBP1b* variant revealed that the PBP1b-LpoB complex is most likely not providing an important physical link between the inner and outer membranes at the division site, as has been previously proposed.
27071112	8	14	theme	important	1646:1654	arg1	link					1665:1668	an important physical link	1643:1668	an important physical link between the inner and outer membranes at the division site	1643:1727	Finally, phenotypic analysis of strains carrying a PBP1b* variant revealed that the PBP1b-LpoB complex is most likely not providing an important physical link between the inner and outer membranes at the division site, as has been previously proposed.
27071112	7	15	theme	change	1379:1384	arg1	induction					1349:1357	the induction	1345:1357	the induction of a conformational change in PBP1b initiated by LpoB binding to its UB2H domain	1345:1438	Moreover, the location of amino acid substitutions causing the bypass phenotype on the PBP1b structure support a model in which polymerization activation proceeds via the induction of a conformational change in PBP1b initiated by LpoB binding to its UB2H domain, followed by its transmission to the glycosyl transferase active site.
27071112	8	16	contain	carrying	1551:1558	arg2	variant					1569:1575	a PBP1b* variant	1560:1575	a PBP1b* variant	1560:1575	Finally, phenotypic analysis of strains carrying a PBP1b* variant revealed that the PBP1b-LpoB complex is most likely not providing an important physical link between the inner and outer membranes at the division site, as has been previously proposed.
27071112	8	16	contain	carrying	1551:1558	arg1	strains					1543:1549	strains	1543:1549	strains carrying a PBP1b* variant	1543:1575	Finally, phenotypic analysis of strains carrying a PBP1b* variant revealed that the PBP1b-LpoB complex is most likely not providing an important physical link between the inner and outer membranes at the division site, as has been previously proposed.
27071112	4	17	theme	PBP1b	671:675	arg1	activity					659:666	the PG polymerization activity	637:666	the PG polymerization activity of PBP1b	637:675	Although it has been demonstrated that LpoB activates the PG polymerization activity of PBP1b in vitro, the mechanism of activation and its physiological relevance have remained unclear.
27071112	7	18	theme	glycosyl	1477:1484	arg1	site					1505:1508	the glycosyl transferase active site	1473:1508	the glycosyl transferase active site	1473:1508	Moreover, the location of amino acid substitutions causing the bypass phenotype on the PBP1b structure support a model in which polymerization activation proceeds via the induction of a conformational change in PBP1b initiated by LpoB binding to its UB2H domain, followed by its transmission to the glycosyl transferase active site.
27071112	7	19	theme	amino	1204:1208	arg1	substitutions					1215:1227	amino acid substitutions	1204:1227	amino acid substitutions causing the bypass phenotype on the PBP1b structure	1204:1279	Moreover, the location of amino acid substitutions causing the bypass phenotype on the PBP1b structure support a model in which polymerization activation proceeds via the induction of a conformational change in PBP1b initiated by LpoB binding to its UB2H domain, followed by its transmission to the glycosyl transferase active site.
27071112	6	20	theme	variants	985:992	arg1	Several					962:968	Several	962:968	Several	962:968	Several of these PBP1b variants were isolated and displayed elevated polymerization activity in vitro, indicating that the activation of glycan polymer growth is indeed one of the relevant functions of LpoB in vivo.
27071112	3	21	theme	membrane	504:511	arg1	LpoA					526:529	LpoA	526:529	LpoA	526:529	We and others have shown that the PG synthases PBP1b and PBP1a of Escherichia coli require the outer membrane lipoproteins LpoA and LpoB, respectively, for their in vivo function.
27071112	3	21	theme	membrane	504:511	arg1	lipoproteins					513:524	the outer membrane lipoproteins	494:524	the outer membrane lipoproteins LpoA and LpoB	494:538	We and others have shown that the PG synthases PBP1b and PBP1a of Escherichia coli require the outer membrane lipoproteins LpoA and LpoB, respectively, for their in vivo function.
27071112	3	21	theme	membrane	504:511	arg1	LpoB					535:538	LpoB	535:538	LpoB	535:538	We and others have shown that the PG synthases PBP1b and PBP1a of Escherichia coli require the outer membrane lipoproteins LpoA and LpoB, respectively, for their in vivo function.
27071112	2	22	theme	penicillin	367:376	arg1	antibiotics					390:400	penicillin and related antibiotics	367:400	penicillin and related antibiotics	367:400	As their name implies, these proteins are the targets of penicillin and related antibiotics.
27071112	2	23	theme	related	382:388	arg1	antibiotics					390:400	penicillin and related antibiotics	367:400	penicillin and related antibiotics	367:400	As their name implies, these proteins are the targets of penicillin and related antibiotics.
27071112	7	24	from	PBP1b	1389:1393	arg1	induction					1349:1357	the induction	1345:1357	the induction of a conformational change in PBP1b initiated by LpoB binding to its UB2H domain	1345:1438	Moreover, the location of amino acid substitutions causing the bypass phenotype on the PBP1b structure support a model in which polymerization activation proceeds via the induction of a conformational change in PBP1b initiated by LpoB binding to its UB2H domain, followed by its transmission to the glycosyl transferase active site.
27071112	7	25	dep	proceeds	1332:1339	arg1	followed					1441:1448	followed	1441:1448	followed by its transmission to the glycosyl transferase active site	1441:1508	Moreover, the location of amino acid substitutions causing the bypass phenotype on the PBP1b structure support a model in which polymerization activation proceeds via the induction of a conformational change in PBP1b initiated by LpoB binding to its UB2H domain, followed by its transmission to the glycosyl transferase active site.
27071112	7	26	theme	bypass	1241:1246	arg1	phenotype					1248:1256	the bypass phenotype	1237:1256	the bypass phenotype on the PBP1b structure	1237:1279	Moreover, the location of amino acid substitutions causing the bypass phenotype on the PBP1b structure support a model in which polymerization activation proceeds via the induction of a conformational change in PBP1b initiated by LpoB binding to its UB2H domain, followed by its transmission to the glycosyl transferase active site.
27071112	4	27	theme	relevance	737:745	arg1	mechanism					691:699	the mechanism	687:699	the mechanism of activation and its physiological relevance	687:745	Although it has been demonstrated that LpoB activates the PG polymerization activity of PBP1b in vitro, the mechanism of activation and its physiological relevance have remained unclear.
27071112	6	28	theme	polymerization	1031:1044	arg1	activity					1046:1053	elevated polymerization activity	1022:1053	elevated polymerization activity	1022:1053	Several of these PBP1b variants were isolated and displayed elevated polymerization activity in vitro, indicating that the activation of glycan polymer growth is indeed one of the relevant functions of LpoB in vivo.
27071112	7	29	theme	PBP1b	1265:1269	arg1	structure					1271:1279	the PBP1b structure	1261:1279	the PBP1b structure	1261:1279	Moreover, the location of amino acid substitutions causing the bypass phenotype on the PBP1b structure support a model in which polymerization activation proceeds via the induction of a conformational change in PBP1b initiated by LpoB binding to its UB2H domain, followed by its transmission to the glycosyl transferase active site.
27071112	6	30	theme	polymer	1106:1112	arg1	growth					1114:1119	glycan polymer growth	1099:1119	glycan polymer growth	1099:1119	Several of these PBP1b variants were isolated and displayed elevated polymerization activity in vitro, indicating that the activation of glycan polymer growth is indeed one of the relevant functions of LpoB in vivo.
27071112	1	31	theme	most	183:186	arg1	bacteria					188:195	most bacteria	183:195	most bacteria	183:195	To fortify their cytoplasmic membrane and protect it from osmotic rupture, most bacteria surround themselves with a peptidoglycan (PG) exoskeleton synthesized by the penicillin-binding proteins (PBPs).
27071112	0	32	theme	Cofactor	0:7	arg1	variants					16:23	Cofactor bypass variants	0:23	Cofactor bypass variants	0:23	Cofactor bypass variants reveal a conformational control mechanism governing cell wall polymerase activity.
27071112	1	33	theme	penicillin-binding	274:291	arg1	PBPs					303:306	PBPs	303:306	PBPs	303:306	To fortify their cytoplasmic membrane and protect it from osmotic rupture, most bacteria surround themselves with a peptidoglycan (PG) exoskeleton synthesized by the penicillin-binding proteins (PBPs).
27071112	1	33	theme	penicillin-binding	274:291	arg1	proteins					293:300	the penicillin-binding proteins	270:300	the penicillin-binding proteins (PBPs)	270:307	To fortify their cytoplasmic membrane and protect it from osmotic rupture, most bacteria surround themselves with a peptidoglycan (PG) exoskeleton synthesized by the penicillin-binding proteins (PBPs).
27071112	2	34	dep	name	319:322	arg1	implies					324:330	implies	324:330	implies	324:330	As their name implies, these proteins are the targets of penicillin and related antibiotics.
27071112	8	35	theme	physical	1656:1663	arg1	link					1665:1668	an important physical link	1643:1668	an important physical link between the inner and outer membranes at the division site	1643:1727	Finally, phenotypic analysis of strains carrying a PBP1b* variant revealed that the PBP1b-LpoB complex is most likely not providing an important physical link between the inner and outer membranes at the division site, as has been previously proposed.
27071112	7	36	theme	polymerization	1306:1319	arg1	activation					1321:1330	polymerization activation	1306:1330	polymerization activation	1306:1330	Moreover, the location of amino acid substitutions causing the bypass phenotype on the PBP1b structure support a model in which polymerization activation proceeds via the induction of a conformational change in PBP1b initiated by LpoB binding to its UB2H domain, followed by its transmission to the glycosyl transferase active site.
27071112	6	37	theme	glycan	1099:1104	arg1	growth					1114:1119	glycan polymer growth	1099:1119	glycan polymer growth	1099:1119	Several of these PBP1b variants were isolated and displayed elevated polymerization activity in vitro, indicating that the activation of glycan polymer growth is indeed one of the relevant functions of LpoB in vivo.
27071112	8	38	from	site	1724:1727	arg1	membranes					1698:1706	the inner and outer membranes	1678:1706	the inner and outer membranes at the division site	1678:1727	Finally, phenotypic analysis of strains carrying a PBP1b* variant revealed that the PBP1b-LpoB complex is most likely not providing an important physical link between the inner and outer membranes at the division site, as has been previously proposed.
27071112	5	39	dep	in	860:861	arg1	vivo					863:866	vivo	863:866	vivo	863:866	We therefore selected for variants of PBP1b (PBP1b*) that bypass the LpoB requirement for in vivo function, reasoning that they would shed light on LpoB function and its activation mechanism.
27071112	4	40	theme	activation	704:713	arg1	mechanism					691:699	the mechanism	687:699	the mechanism of activation and its physiological relevance	687:745	Although it has been demonstrated that LpoB activates the PG polymerization activity of PBP1b in vitro, the mechanism of activation and its physiological relevance have remained unclear.
27071112	5	41	theme	PBP1b	808:812	arg1	variants					796:803	variants	796:803	variants of PBP1b (PBP1b*) that bypass the LpoB requirement for in vivo function	796:875	We therefore selected for variants of PBP1b (PBP1b*) that bypass the LpoB requirement for in vivo function, reasoning that they would shed light on LpoB function and its activation mechanism.
27071112	0	42	theme	conformational	34:47	arg1	mechanism					57:65	a conformational control mechanism	32:65	a conformational control mechanism governing cell wall polymerase activity	32:105	Cofactor bypass variants reveal a conformational control mechanism governing cell wall polymerase activity.
27071112	3	43	dep	lipoproteins	513:524	arg1	LpoA					526:529	LpoA	526:529	LpoA	526:529	We and others have shown that the PG synthases PBP1b and PBP1a of Escherichia coli require the outer membrane lipoproteins LpoA and LpoB, respectively, for their in vivo function.
27071112	3	43	dep	lipoproteins	513:524	arg1	lipoproteins					513:524	the outer membrane lipoproteins	494:524	the outer membrane lipoproteins LpoA and LpoB	494:538	We and others have shown that the PG synthases PBP1b and PBP1a of Escherichia coli require the outer membrane lipoproteins LpoA and LpoB, respectively, for their in vivo function.
27071112	3	43	dep	lipoproteins	513:524	arg1	LpoB					535:538	LpoB	535:538	LpoB	535:538	We and others have shown that the PG synthases PBP1b and PBP1a of Escherichia coli require the outer membrane lipoproteins LpoA and LpoB, respectively, for their in vivo function.
27071112	7	44	theme	substitutions	1215:1227	arg1	location					1192:1199	the location	1188:1199	the location of amino acid substitutions causing the bypass phenotype on the PBP1b structure	1188:1279	Moreover, the location of amino acid substitutions causing the bypass phenotype on the PBP1b structure support a model in which polymerization activation proceeds via the induction of a conformational change in PBP1b initiated by LpoB binding to its UB2H domain, followed by its transmission to the glycosyl transferase active site.
27071112	3	45	theme	in	565:566	arg1	function					573:580	their in vivo function	559:580	their in vivo function	559:580	We and others have shown that the PG synthases PBP1b and PBP1a of Escherichia coli require the outer membrane lipoproteins LpoA and LpoB, respectively, for their in vivo function.
27071112	6	46	theme	PBP1b	979:983	arg1	variants					985:992	these PBP1b variants	973:992	these PBP1b variants	973:992	Several of these PBP1b variants were isolated and displayed elevated polymerization activity in vitro, indicating that the activation of glycan polymer growth is indeed one of the relevant functions of LpoB in vivo.
27071112	6	47	theme	elevated	1022:1029	arg1	activity					1046:1053	elevated polymerization activity	1022:1053	elevated polymerization activity	1022:1053	Several of these PBP1b variants were isolated and displayed elevated polymerization activity in vitro, indicating that the activation of glycan polymer growth is indeed one of the relevant functions of LpoB in vivo.
27071112	8	48	theme	strains	1543:1549	arg1	analysis					1531:1538	phenotypic analysis	1520:1538	phenotypic analysis of strains carrying a PBP1b* variant	1520:1575	Finally, phenotypic analysis of strains carrying a PBP1b* variant revealed that the PBP1b-LpoB complex is most likely not providing an important physical link between the inner and outer membranes at the division site, as has been previously proposed.
27071112	5	49	theme	PBP1b	815:819	arg1	PBP1b					808:812	PBP1b	808:812	PBP1b (PBP1b*) that bypass the LpoB requirement for in vivo function	808:875	We therefore selected for variants of PBP1b (PBP1b*) that bypass the LpoB requirement for in vivo function, reasoning that they would shed light on LpoB function and its activation mechanism.
27071112	5	49	theme	PBP1b	815:819	arg1	*					820:820	PBP1b*	815:820	PBP1b*	815:820	We therefore selected for variants of PBP1b (PBP1b*) that bypass the LpoB requirement for in vivo function, reasoning that they would shed light on LpoB function and its activation mechanism.
27071112	3	50	theme	PG	437:438	arg1	synthases					440:448	the PG synthases PBP1b and PBP1a	433:464	the PG synthases PBP1b and PBP1a of Escherichia coli	433:484	We and others have shown that the PG synthases PBP1b and PBP1a of Escherichia coli require the outer membrane lipoproteins LpoA and LpoB, respectively, for their in vivo function.
27071112	3	50	theme	PG	437:438	arg1	PBP1a					460:464	PBP1a	460:464	PBP1a	460:464	We and others have shown that the PG synthases PBP1b and PBP1a of Escherichia coli require the outer membrane lipoproteins LpoA and LpoB, respectively, for their in vivo function.
27071112	3	50	theme	PG	437:438	arg1	PBP1b					450:454	PBP1b	450:454	PBP1b	450:454	We and others have shown that the PG synthases PBP1b and PBP1a of Escherichia coli require the outer membrane lipoproteins LpoA and LpoB, respectively, for their in vivo function.
27071112	4	51	theme	PG	641:642	arg1	activity					659:666	the PG polymerization activity	637:666	the PG polymerization activity of PBP1b	637:675	Although it has been demonstrated that LpoB activates the PG polymerization activity of PBP1b in vitro, the mechanism of activation and its physiological relevance have remained unclear.
27071112	6	52	theme	LpoB	1164:1167	arg1	functions					1151:1159	the relevant functions	1138:1159	the relevant functions of LpoB in vivo	1138:1175	Several of these PBP1b variants were isolated and displayed elevated polymerization activity in vitro, indicating that the activation of glycan polymer growth is indeed one of the relevant functions of LpoB in vivo.
27071112	5	53	theme	in	860:861	arg1	function					868:875	in vivo function	860:875	in vivo function	860:875	We therefore selected for variants of PBP1b (PBP1b*) that bypass the LpoB requirement for in vivo function, reasoning that they would shed light on LpoB function and its activation mechanism.
27071112	0	54	theme	control	49:55	arg1	mechanism					57:65	a conformational control mechanism	32:65	a conformational control mechanism governing cell wall polymerase activity	32:105	Cofactor bypass variants reveal a conformational control mechanism governing cell wall polymerase activity.
27071112	8	55	theme	outer	1692:1696	arg1	membranes					1698:1706	the inner and outer membranes	1678:1706	the inner and outer membranes at the division site	1678:1727	Finally, phenotypic analysis of strains carrying a PBP1b* variant revealed that the PBP1b-LpoB complex is most likely not providing an important physical link between the inner and outer membranes at the division site, as has been previously proposed.
27071112	1	56	theme	cytoplasmic	125:135	arg1	membrane					137:144	their cytoplasmic membrane	119:144	their cytoplasmic membrane	119:144	To fortify their cytoplasmic membrane and protect it from osmotic rupture, most bacteria surround themselves with a peptidoglycan (PG) exoskeleton synthesized by the penicillin-binding proteins (PBPs).
27071112	7	57	theme	conformational	1364:1377	arg1	change					1379:1384	a conformational change	1362:1384	a conformational change in PBP1b initiated by LpoB binding to its UB2H domain	1362:1438	Moreover, the location of amino acid substitutions causing the bypass phenotype on the PBP1b structure support a model in which polymerization activation proceeds via the induction of a conformational change in PBP1b initiated by LpoB binding to its UB2H domain, followed by its transmission to the glycosyl transferase active site.
27071112	8	58	theme	inner	1682:1686	arg1	membranes					1698:1706	the inner and outer membranes	1678:1706	the inner and outer membranes at the division site	1678:1727	Finally, phenotypic analysis of strains carrying a PBP1b* variant revealed that the PBP1b-LpoB complex is most likely not providing an important physical link between the inner and outer membranes at the division site, as has been previously proposed.
27071112	6	59	theme	functions	1151:1159	arg1	activation					1085:1094	the activation	1081:1094	the activation of glycan polymer growth	1081:1119	Several of these PBP1b variants were isolated and displayed elevated polymerization activity in vitro, indicating that the activation of glycan polymer growth is indeed one of the relevant functions of LpoB in vivo.
27071112	6	59	theme	functions	1151:1159	arg1	functions					1151:1159	the relevant functions	1138:1159	the relevant functions of LpoB in vivo	1138:1175	Several of these PBP1b variants were isolated and displayed elevated polymerization activity in vitro, indicating that the activation of glycan polymer growth is indeed one of the relevant functions of LpoB in vivo.
27071112	6	59	theme	functions	1151:1159	arg1	one					1131:1133	one	1131:1133	one	1131:1133	Several of these PBP1b variants were isolated and displayed elevated polymerization activity in vitro, indicating that the activation of glycan polymer growth is indeed one of the relevant functions of LpoB in vivo.
27071112	4	60	theme	polymerization	644:657	arg1	activity					659:666	the PG polymerization activity	637:666	the PG polymerization activity of PBP1b	637:675	Although it has been demonstrated that LpoB activates the PG polymerization activity of PBP1b in vitro, the mechanism of activation and its physiological relevance have remained unclear.
27071112	8	61	theme	PBP1b	1562:1566	arg1	variant					1569:1575	a PBP1b* variant	1560:1575	a PBP1b* variant	1560:1575	Finally, phenotypic analysis of strains carrying a PBP1b* variant revealed that the PBP1b-LpoB complex is most likely not providing an important physical link between the inner and outer membranes at the division site, as has been previously proposed.
27071112	1	62	theme	peptidoglycan	224:236	arg1	exoskeleton					243:253	a peptidoglycan (PG) exoskeleton	222:253	a peptidoglycan (PG) exoskeleton synthesized by the penicillin-binding proteins (PBPs)	222:307	To fortify their cytoplasmic membrane and protect it from osmotic rupture, most bacteria surround themselves with a peptidoglycan (PG) exoskeleton synthesized by the penicillin-binding proteins (PBPs).
27071112	5	63	theme	LpoB	918:921	arg1	function					923:930	LpoB function	918:930	LpoB function	918:930	We therefore selected for variants of PBP1b (PBP1b*) that bypass the LpoB requirement for in vivo function, reasoning that they would shed light on LpoB function and its activation mechanism.
27071112	7	64	theme	acid	1210:1213	arg1	substitutions					1215:1227	amino acid substitutions	1204:1227	amino acid substitutions causing the bypass phenotype on the PBP1b structure	1204:1279	Moreover, the location of amino acid substitutions causing the bypass phenotype on the PBP1b structure support a model in which polymerization activation proceeds via the induction of a conformational change in PBP1b initiated by LpoB binding to its UB2H domain, followed by its transmission to the glycosyl transferase active site.
27071112	0	65	dep	variants	16:23	arg1	bypass					9:14	bypass	9:14	bypass	9:14	Cofactor bypass variants reveal a conformational control mechanism governing cell wall polymerase activity.
27071112	7	66	from	phenotype	1248:1256	arg1	structure					1271:1279	the PBP1b structure	1261:1279	the PBP1b structure	1261:1279	Moreover, the location of amino acid substitutions causing the bypass phenotype on the PBP1b structure support a model in which polymerization activation proceeds via the induction of a conformational change in PBP1b initiated by LpoB binding to its UB2H domain, followed by its transmission to the glycosyl transferase active site.
27071112	3	67	dep	in	565:566	arg1	vivo					568:571	vivo	568:571	vivo	568:571	We and others have shown that the PG synthases PBP1b and PBP1a of Escherichia coli require the outer membrane lipoproteins LpoA and LpoB, respectively, for their in vivo function.
27071112	8	68	theme	phenotypic	1520:1529	arg1	analysis					1531:1538	phenotypic analysis	1520:1538	phenotypic analysis of strains carrying a PBP1b* variant	1520:1575	Finally, phenotypic analysis of strains carrying a PBP1b* variant revealed that the PBP1b-LpoB complex is most likely not providing an important physical link between the inner and outer membranes at the division site, as has been previously proposed.
27071112	0	69	theme	cell	77:80	arg1	activity					98:105	cell wall polymerase activity	77:105	cell wall polymerase activity	77:105	Cofactor bypass variants reveal a conformational control mechanism governing cell wall polymerase activity.
27071112	2	70	theme	antibiotics	390:400	arg1	proteins					339:346	these proteins	333:346	these proteins	333:346	As their name implies, these proteins are the targets of penicillin and related antibiotics.
27071112	2	70	theme	antibiotics	390:400	arg1	targets					356:362	the targets	352:362	the targets of penicillin and related antibiotics	352:400	As their name implies, these proteins are the targets of penicillin and related antibiotics.
27071112	3	71	dep	synthases	440:448	arg1	synthases					440:448	the PG synthases PBP1b and PBP1a	433:464	the PG synthases PBP1b and PBP1a of Escherichia coli	433:484	We and others have shown that the PG synthases PBP1b and PBP1a of Escherichia coli require the outer membrane lipoproteins LpoA and LpoB, respectively, for their in vivo function.
27071112	3	71	dep	synthases	440:448	arg1	PBP1a					460:464	PBP1a	460:464	PBP1a	460:464	We and others have shown that the PG synthases PBP1b and PBP1a of Escherichia coli require the outer membrane lipoproteins LpoA and LpoB, respectively, for their in vivo function.
27071112	3	71	dep	synthases	440:448	arg1	PBP1b					450:454	PBP1b	450:454	PBP1b	450:454	We and others have shown that the PG synthases PBP1b and PBP1a of Escherichia coli require the outer membrane lipoproteins LpoA and LpoB, respectively, for their in vivo function.
27071112	5	72	theme	LpoB	839:842	arg1	requirement					844:854	the LpoB requirement	835:854	the LpoB requirement for in vivo function	835:875	We therefore selected for variants of PBP1b (PBP1b*) that bypass the LpoB requirement for in vivo function, reasoning that they would shed light on LpoB function and its activation mechanism.
27071112	7	73	from	induction	1349:1357	arg1	PBP1b					1389:1393	PBP1b	1389:1393	PBP1b initiated by LpoB binding to its UB2H domain	1389:1438	Moreover, the location of amino acid substitutions causing the bypass phenotype on the PBP1b structure support a model in which polymerization activation proceeds via the induction of a conformational change in PBP1b initiated by LpoB binding to its UB2H domain, followed by its transmission to the glycosyl transferase active site.
27071112	1	74	theme	PG	239:240	arg1	exoskeleton					243:253	a peptidoglycan (PG) exoskeleton	222:253	a peptidoglycan (PG) exoskeleton synthesized by the penicillin-binding proteins (PBPs)	222:307	To fortify their cytoplasmic membrane and protect it from osmotic rupture, most bacteria surround themselves with a peptidoglycan (PG) exoskeleton synthesized by the penicillin-binding proteins (PBPs).
27071112	5	75	theme	activation	940:949	arg1	mechanism					951:959	its activation mechanism	936:959	its activation mechanism	936:959	We therefore selected for variants of PBP1b (PBP1b*) that bypass the LpoB requirement for in vivo function, reasoning that they would shed light on LpoB function and its activation mechanism.
27071112	8	76	theme	division	1715:1722	arg1	site					1724:1727	the division site	1711:1727	the division site	1711:1727	Finally, phenotypic analysis of strains carrying a PBP1b* variant revealed that the PBP1b-LpoB complex is most likely not providing an important physical link between the inner and outer membranes at the division site, as has been previously proposed.
27071112	3	77	theme	outer	498:502	arg1	LpoA					526:529	LpoA	526:529	LpoA	526:529	We and others have shown that the PG synthases PBP1b and PBP1a of Escherichia coli require the outer membrane lipoproteins LpoA and LpoB, respectively, for their in vivo function.
27071112	3	77	theme	outer	498:502	arg1	lipoproteins					513:524	the outer membrane lipoproteins	494:524	the outer membrane lipoproteins LpoA and LpoB	494:538	We and others have shown that the PG synthases PBP1b and PBP1a of Escherichia coli require the outer membrane lipoproteins LpoA and LpoB, respectively, for their in vivo function.
27071112	3	77	theme	outer	498:502	arg1	LpoB					535:538	LpoB	535:538	LpoB	535:538	We and others have shown that the PG synthases PBP1b and PBP1a of Escherichia coli require the outer membrane lipoproteins LpoA and LpoB, respectively, for their in vivo function.
25185503	4	0	theme	different	518:526	arg1	radioisotopes					528:540	different radioisotopes	518:540	different radioisotopes	518:540	Aptamers can be labeled with different radioisotopes and possess several properties that make them suitable for molecular imaging.
25185503	8	1	theme	high	1043:1046	arg1	affinity					1048:1055	high affinity	1043:1055	high affinity for Staphylococcus aureus cells	1043:1087	These aptamers labeled with (32)P showed high affinity for Staphylococcus aureus cells.
25185503	6	2	theme	Enrichment	866:875	arg1	methodology					885:895	Exponential Enrichment (SELEX) methodology	854:895	Exponential Enrichment (SELEX) methodology	854:895	Two aptamers (Antibac1 and Antibac2) against peptidoglycan were selected through the Systematic Evolution of Ligands by Exponential Enrichment (SELEX) methodology.
25185503	8	3	theme	Staphylococcus	1061:1074	arg1	cells					1083:1087	Staphylococcus aureus cells	1061:1087	Staphylococcus aureus cells	1061:1087	These aptamers labeled with (32)P showed high affinity for Staphylococcus aureus cells.
25185503	6	4	theme	Exponential	854:864	arg1	SELEX					878:882	SELEX	878:882	SELEX	878:882	Two aptamers (Antibac1 and Antibac2) against peptidoglycan were selected through the Systematic Evolution of Ligands by Exponential Enrichment (SELEX) methodology.
25185503	6	4	theme	Exponential	854:864	arg1	Enrichment					866:875	Exponential Enrichment	854:875	Exponential Enrichment (SELEX) methodology	854:895	Two aptamers (Antibac1 and Antibac2) against peptidoglycan were selected through the Systematic Evolution of Ligands by Exponential Enrichment (SELEX) methodology.
25185503	2	5	contain	has	268:270	arg1	Peptidoglycan					254:266	Peptidoglycan	254:266	Peptidoglycan	254:266	Peptidoglycan has a particular composition which makes it a possible target for specific bacterial recognition.
25185503	2	5	contain	has	268:270	arg2	composition					285:295	a particular composition	272:295	a particular composition which makes it a possible target for specific bacterial recognition	272:363	Peptidoglycan has a particular composition which makes it a possible target for specific bacterial recognition.
25185503	3	6	theme	single-stranded	379:393	arg1	Aptamers					366:373	Aptamers	366:373	Aptamers	366:373	Aptamers are single-stranded DNA or RNA oligonucleotides that bind to target molecules with high affinity and specificity.
25185503	3	6	theme	single-stranded	379:393	arg1	DNA					395:397	single-stranded DNA	379:397	single-stranded DNA	379:397	Aptamers are single-stranded DNA or RNA oligonucleotides that bind to target molecules with high affinity and specificity.
25185503	5	7	theme	study	640:644	arg1	purpose					624:630	The purpose	620:630	The purpose of this study	620:644	The purpose of this study was to obtain aptamers for use as radiopharmaceutical in bacterial infection diagnosis.
25185503	7	8	theme	dissociation	902:913	arg1	μM					960:961	0.415 + 0.047 μM	946:961	0.415 + 0.047 μM	946:961	The dissociation constant (Kd) for Antibac1 was 0.415 + 0.047 μM and for Antibac2 was 1.261 + 0.280 μM.
25185503	7	8	theme	dissociation	902:913	arg1	Kd					925:926	Kd	925:926	Kd	925:926	The dissociation constant (Kd) for Antibac1 was 0.415 + 0.047 μM and for Antibac2 was 1.261 + 0.280 μM.
25185503	7	8	theme	dissociation	902:913	arg1	constant					915:922	The dissociation constant	898:922	The dissociation constant (Kd) for Antibac1	898:940	The dissociation constant (Kd) for Antibac1 was 0.415 + 0.047 μM and for Antibac2 was 1.261 + 0.280 μM.
25185503	6	9	theme	Ligands	843:849	arg1	Evolution					830:838	the Systematic Evolution	815:838	the Systematic Evolution of Ligands by Exponential Enrichment (SELEX) methodology	815:895	Two aptamers (Antibac1 and Antibac2) against peptidoglycan were selected through the Systematic Evolution of Ligands by Exponential Enrichment (SELEX) methodology.
25185503	10	10	theme	promising	1318:1326	arg1	Antibac2					1306:1313	Antibac2	1306:1313	Antibac2	1306:1313	These results point Antibac1 and Antibac2 as promising tools for bacterial infections identification.
25185503	10	10	theme	promising	1318:1326	arg1	tools					1328:1332	promising tools	1318:1332	promising tools for bacterial infections identification	1318:1372	These results point Antibac1 and Antibac2 as promising tools for bacterial infections identification.
25185503	10	10	theme	promising	1318:1326	arg1	Antibac1					1293:1300	Antibac1	1293:1300	Antibac1	1293:1300	These results point Antibac1 and Antibac2 as promising tools for bacterial infections identification.
25185503	3	11	theme	high	458:461	arg1	affinity					463:470	high affinity	458:470	high affinity	458:470	Aptamers are single-stranded DNA or RNA oligonucleotides that bind to target molecules with high affinity and specificity.
25185503	8	12	theme	aureus	1076:1081	arg1	cells					1083:1087	Staphylococcus aureus cells	1061:1087	Staphylococcus aureus cells	1061:1087	These aptamers labeled with (32)P showed high affinity for Staphylococcus aureus cells.
25185503	6	13	dep	aptamers	738:745	arg1	aptamers					738:745	Two aptamers	734:745	Two aptamers (Antibac1 and Antibac2) against peptidoglycan	734:791	Two aptamers (Antibac1 and Antibac2) against peptidoglycan were selected through the Systematic Evolution of Ligands by Exponential Enrichment (SELEX) methodology.
25185503	6	13	dep	aptamers	738:745	arg1	Antibac2					761:768	Antibac2	761:768	Antibac2	761:768	Two aptamers (Antibac1 and Antibac2) against peptidoglycan were selected through the Systematic Evolution of Ligands by Exponential Enrichment (SELEX) methodology.
25185503	6	13	dep	aptamers	738:745	arg1	Antibac1					748:755	Antibac1	748:755	Antibac1	748:755	Two aptamers (Antibac1 and Antibac2) against peptidoglycan were selected through the Systematic Evolution of Ligands by Exponential Enrichment (SELEX) methodology.
25185503	2	14	theme	particular	274:283	arg1	composition					285:295	a particular composition	272:295	a particular composition which makes it a possible target for specific bacterial recognition	272:363	Peptidoglycan has a particular composition which makes it a possible target for specific bacterial recognition.
25185503	3	15	theme	RNA	402:404	arg1	oligonucleotides					406:421	RNA oligonucleotides	402:421	RNA oligonucleotides	402:421	Aptamers are single-stranded DNA or RNA oligonucleotides that bind to target molecules with high affinity and specificity.
25185503	6	16	theme	Systematic	819:828	arg1	Evolution					830:838	the Systematic Evolution	815:838	the Systematic Evolution of Ligands by Exponential Enrichment (SELEX) methodology	815:895	Two aptamers (Antibac1 and Antibac2) against peptidoglycan were selected through the Systematic Evolution of Ligands by Exponential Enrichment (SELEX) methodology.
25185503	2	17	theme	possible	314:321	arg1	target					323:328	a possible target	312:328	a possible target for specific bacterial recognition	312:363	Peptidoglycan has a particular composition which makes it a possible target for specific bacterial recognition.
25185503	0	18	theme	aptamers	36:43	arg1	Selection					0:8	Selection	0:8	Selection of peptidoglycan-specific aptamers for bacterial cells	0:63	Selection of peptidoglycan-specific aptamers for bacterial cells identification.
25185503	10	19	theme	infections	1348:1357	arg1	identification					1359:1372	bacterial infections identification	1338:1372	bacterial infections identification	1338:1372	These results point Antibac1 and Antibac2 as promising tools for bacterial infections identification.
25185503	1	20	theme	complex	107:113	arg1	macromolecule					129:141	a highly complex and essential macromolecule	98:141	a highly complex and essential macromolecule of bacterial outer cell wall	98:170	Peptidoglycan is a highly complex and essential macromolecule of bacterial outer cell wall; it is a heteropolymer made up of linear glycan strands cross-linked by peptides.
25185503	1	20	theme	complex	107:113	arg1	Peptidoglycan					81:93	Peptidoglycan	81:93	Peptidoglycan	81:93	Peptidoglycan is a highly complex and essential macromolecule of bacterial outer cell wall; it is a heteropolymer made up of linear glycan strands cross-linked by peptides.
25185503	4	21	theme	molecular	601:609	arg1	imaging					611:617	molecular imaging	601:617	molecular imaging	601:617	Aptamers can be labeled with different radioisotopes and possess several properties that make them suitable for molecular imaging.
25185503	9	22	theme	bacterial	1256:1264	arg1	cells					1266:1270	bacterial cells	1256:1270	bacterial cells	1256:1270	The binding to S. aureus and Escherichia coli in vitro were significantly higher than for Candida albicans and human fibroblasts, demonstrating their specificity for bacterial cells.
25185503	1	23	theme	essential	119:127	arg1	macromolecule					129:141	a highly complex and essential macromolecule	98:141	a highly complex and essential macromolecule of bacterial outer cell wall	98:170	Peptidoglycan is a highly complex and essential macromolecule of bacterial outer cell wall; it is a heteropolymer made up of linear glycan strands cross-linked by peptides.
25185503	1	23	theme	essential	119:127	arg1	Peptidoglycan					81:93	Peptidoglycan	81:93	Peptidoglycan	81:93	Peptidoglycan is a highly complex and essential macromolecule of bacterial outer cell wall; it is a heteropolymer made up of linear glycan strands cross-linked by peptides.
25185503	10	24	theme	bacterial	1338:1346	arg1	identification					1359:1372	bacterial infections identification	1338:1372	bacterial infections identification	1338:1372	These results point Antibac1 and Antibac2 as promising tools for bacterial infections identification.
25185503	0	25	theme	bacterial	49:57	arg1	cells					59:63	bacterial cells	49:63	bacterial cells	49:63	Selection of peptidoglycan-specific aptamers for bacterial cells identification.
25185503	0	26	dep	identification	65:78	arg1	Selection					0:8	Selection	0:8	Selection of peptidoglycan-specific aptamers for bacterial cells	0:63	Selection of peptidoglycan-specific aptamers for bacterial cells identification.
25185503	1	27	theme	linear	206:211	arg1	strands					220:226	linear glycan strands	206:226	linear glycan strands cross-linked by peptides	206:251	Peptidoglycan is a highly complex and essential macromolecule of bacterial outer cell wall; it is a heteropolymer made up of linear glycan strands cross-linked by peptides.
25185503	3	28	theme	target	436:441	arg1	molecules					443:451	target molecules	436:451	target molecules	436:451	Aptamers are single-stranded DNA or RNA oligonucleotides that bind to target molecules with high affinity and specificity.
25185503	5	29	from	radiopharmaceutical	680:698	arg1	diagnosis					723:731	bacterial infection diagnosis	703:731	bacterial infection diagnosis	703:731	The purpose of this study was to obtain aptamers for use as radiopharmaceutical in bacterial infection diagnosis.
25185503	1	30	theme	glycan	213:218	arg1	strands					220:226	linear glycan strands	206:226	linear glycan strands cross-linked by peptides	206:251	Peptidoglycan is a highly complex and essential macromolecule of bacterial outer cell wall; it is a heteropolymer made up of linear glycan strands cross-linked by peptides.
25185503	4	31	theme	several	554:560	arg1	properties					562:571	several properties	554:571	several properties that make them suitable for molecular imaging	554:617	Aptamers can be labeled with different radioisotopes and possess several properties that make them suitable for molecular imaging.
25185503	5	32	theme	bacterial	703:711	arg1	diagnosis					723:731	bacterial infection diagnosis	703:731	bacterial infection diagnosis	703:731	The purpose of this study was to obtain aptamers for use as radiopharmaceutical in bacterial infection diagnosis.
25185503	9	33	dep	aureus	1108:1113	arg1	vitro					1139:1143	in vitro	1136:1143	in vitro	1136:1143	The binding to S. aureus and Escherichia coli in vitro were significantly higher than for Candida albicans and human fibroblasts, demonstrating their specificity for bacterial cells.
25185503	5	34	theme	infection	713:721	arg1	diagnosis					723:731	bacterial infection diagnosis	703:731	bacterial infection diagnosis	703:731	The purpose of this study was to obtain aptamers for use as radiopharmaceutical in bacterial infection diagnosis.
25185503	1	35	theme	wall	167:170	arg1	macromolecule					129:141	a highly complex and essential macromolecule	98:141	a highly complex and essential macromolecule of bacterial outer cell wall	98:170	Peptidoglycan is a highly complex and essential macromolecule of bacterial outer cell wall; it is a heteropolymer made up of linear glycan strands cross-linked by peptides.
25185503	1	35	theme	wall	167:170	arg1	Peptidoglycan					81:93	Peptidoglycan	81:93	Peptidoglycan	81:93	Peptidoglycan is a highly complex and essential macromolecule of bacterial outer cell wall; it is a heteropolymer made up of linear glycan strands cross-linked by peptides.
25185503	2	36	theme	bacterial	343:351	arg1	recognition					353:363	specific bacterial recognition	334:363	specific bacterial recognition	334:363	Peptidoglycan has a particular composition which makes it a possible target for specific bacterial recognition.
25185503	9	37	theme	human	1201:1205	arg1	fibroblasts					1207:1217	human fibroblasts	1201:1217	human fibroblasts	1201:1217	The binding to S. aureus and Escherichia coli in vitro were significantly higher than for Candida albicans and human fibroblasts, demonstrating their specificity for bacterial cells.
25185503	1	38	theme	bacterial	146:154	arg1	wall					167:170	bacterial outer cell wall	146:170	bacterial outer cell wall	146:170	Peptidoglycan is a highly complex and essential macromolecule of bacterial outer cell wall; it is a heteropolymer made up of linear glycan strands cross-linked by peptides.
25185503	1	39	theme	outer	156:160	arg1	wall					167:170	bacterial outer cell wall	146:170	bacterial outer cell wall	146:170	Peptidoglycan is a highly complex and essential macromolecule of bacterial outer cell wall; it is a heteropolymer made up of linear glycan strands cross-linked by peptides.
25185503	2	40	theme	specific	334:341	arg1	recognition					353:363	specific bacterial recognition	334:363	specific bacterial recognition	334:363	Peptidoglycan has a particular composition which makes it a possible target for specific bacterial recognition.
25185503	1	41	theme	cell	162:165	arg1	wall					167:170	bacterial outer cell wall	146:170	bacterial outer cell wall	146:170	Peptidoglycan is a highly complex and essential macromolecule of bacterial outer cell wall; it is a heteropolymer made up of linear glycan strands cross-linked by peptides.
25185503	0	42	theme	peptidoglycan-specific	13:34	arg1	aptamers					36:43	peptidoglycan-specific aptamers	13:43	peptidoglycan-specific aptamers	13:43	Selection of peptidoglycan-specific aptamers for bacterial cells identification.
25185503	9	43	theme	Candida	1180:1186	arg1	albicans					1188:1195	Candida albicans	1180:1195	Candida albicans	1180:1195	The binding to S. aureus and Escherichia coli in vitro were significantly higher than for Candida albicans and human fibroblasts, demonstrating their specificity for bacterial cells.
25185503	4	44	contain	possess	546:552	arg2	properties					562:571	several properties	554:571	several properties that make them suitable for molecular imaging	554:617	Aptamers can be labeled with different radioisotopes and possess several properties that make them suitable for molecular imaging.
25185503	4	44	contain	possess	546:552	arg1	Aptamers					489:496	Aptamers	489:496	Aptamers	489:496	Aptamers can be labeled with different radioisotopes and possess several properties that make them suitable for molecular imaging.
25347206	7	0	theme	nanoparticle	962:973	arg1	core					981:984	the nanoparticle metal core	958:984	the nanoparticle metal core	958:984	While BSA and IgM interact with the gold nanoparticles by forming electrostatic interactions with the citrate ligands, IgG and hyaluronan adsorb to the nanoparticle metal core by displacing the citrate ligands.
25347206	9	1	theme	cluster	1146:1152	arg1	formation					1154:1162	nanoparticle cluster formation	1133:1162	nanoparticle cluster formation	1133:1162	Human IgG and IgM caused nanoparticle cluster formation upon interacting with the gold nanoparticles.
25347206	1	2	theme	citrate-capped	170:183	arg1	nanoparticles					190:202	citrate-capped gold nanoparticles	170:202	citrate-capped gold nanoparticles	170:202	In this study, we investigated the interaction between five biorelevant molecules and citrate-capped gold nanoparticles using dynamic light scattering, ζ-potential analysis, UV-vis absorption spectroscopy, and transmission electron microscopy.
25347206	7	3	theme	gold	846:849	arg1	nanoparticles					851:863	the gold nanoparticles	842:863	the gold nanoparticles	842:863	While BSA and IgM interact with the gold nanoparticles by forming electrostatic interactions with the citrate ligands, IgG and hyaluronan adsorb to the nanoparticle metal core by displacing the citrate ligands.
25347206	5	4	theme	conditions	683:692	arg1	number					665:670	a number	663:670	a number of medical conditions including rheumatoid arthritis and malignancy	663:738	An abnormal level of hyaluronan in blood is associated with a number of medical conditions including rheumatoid arthritis and malignancy.
25347206	5	4	theme	conditions	683:692	arg1	arthritis					715:723	rheumatoid arthritis	704:723	rheumatoid arthritis	704:723	An abnormal level of hyaluronan in blood is associated with a number of medical conditions including rheumatoid arthritis and malignancy.
25347206	5	4	theme	conditions	683:692	arg1	malignancy					729:738	malignancy	729:738	malignancy	729:738	An abnormal level of hyaluronan in blood is associated with a number of medical conditions including rheumatoid arthritis and malignancy.
25347206	5	5	theme	hyaluronan	624:633	arg1	level					615:619	An abnormal level	603:619	An abnormal level of hyaluronan in blood	603:642	An abnormal level of hyaluronan in blood is associated with a number of medical conditions including rheumatoid arthritis and malignancy.
25347206	3	6	theme	high	508:511	arg1	IgG					491:493	IgG	491:493	IgG	491:493	BSA, IgG, and IgM are high abundance proteins in blood.
25347206	3	6	theme	high	508:511	arg1	IgM					500:502	IgM	500:502	IgM	500:502	BSA, IgG, and IgM are high abundance proteins in blood.
25347206	3	6	theme	high	508:511	arg1	BSA					486:488	BSA	486:488	BSA	486:488	BSA, IgG, and IgM are high abundance proteins in blood.
25347206	3	6	theme	high	508:511	arg1	proteins					523:530	high abundance proteins	508:530	high abundance proteins in blood	508:539	BSA, IgG, and IgM are high abundance proteins in blood.
25347206	9	7	theme	nanoparticle	1133:1144	arg1	formation					1154:1162	nanoparticle cluster formation	1133:1162	nanoparticle cluster formation	1133:1162	Human IgG and IgM caused nanoparticle cluster formation upon interacting with the gold nanoparticles.
25347206	4	8	theme	major	558:562	arg1	Hyaluronan					542:551	Hyaluronan	542:551	Hyaluronan	542:551	Hyaluronan is a major component of the extracellular matrix.
25347206	4	8	theme	major	558:562	arg1	component					564:572	a major component	556:572	a major component of the extracellular matrix	556:600	Hyaluronan is a major component of the extracellular matrix.
25347206	7	9	theme	citrate	1004:1010	arg1	ligands					1012:1018	the citrate ligands	1000:1018	the citrate ligands	1000:1018	While BSA and IgM interact with the gold nanoparticles by forming electrostatic interactions with the citrate ligands, IgG and hyaluronan adsorb to the nanoparticle metal core by displacing the citrate ligands.
25347206	3	10	theme	abundance	513:521	arg1	IgG					491:493	IgG	491:493	IgG	491:493	BSA, IgG, and IgM are high abundance proteins in blood.
25347206	3	10	theme	abundance	513:521	arg1	IgM					500:502	IgM	500:502	IgM	500:502	BSA, IgG, and IgM are high abundance proteins in blood.
25347206	3	10	theme	abundance	513:521	arg1	BSA					486:488	BSA	486:488	BSA	486:488	BSA, IgG, and IgM are high abundance proteins in blood.
25347206	3	10	theme	abundance	513:521	arg1	proteins					523:530	high abundance proteins	508:530	high abundance proteins in blood	508:539	BSA, IgG, and IgM are high abundance proteins in blood.
25347206	12	11	theme	biological	1654:1663	arg1	fluids					1665:1670	other biological fluids	1648:1670	other biological fluids	1648:1670	This finding has exciting implications for future research: the molecular composition of a protein corona formed on a nanoparticle surface upon mixing the nanoparticle with blood or other biological fluids may vary according to the pathological conditions of individuals, and the analysis of these compositions could potentially lead to new biomarker discovery with diagnostic applications.
25347206	1	12	theme	transmission	294:305	arg1	microscopy					316:325	transmission electron microscopy	294:325	transmission electron microscopy	294:325	In this study, we investigated the interaction between five biorelevant molecules and citrate-capped gold nanoparticles using dynamic light scattering, ζ-potential analysis, UV-vis absorption spectroscopy, and transmission electron microscopy.
25347206	5	13	theme	rheumatoid	704:713	arg1	arthritis					715:723	rheumatoid arthritis	704:723	rheumatoid arthritis	704:723	An abnormal level of hyaluronan in blood is associated with a number of medical conditions including rheumatoid arthritis and malignancy.
25347206	4	14	theme	matrix	595:600	arg1	Hyaluronan					542:551	Hyaluronan	542:551	Hyaluronan	542:551	Hyaluronan is a major component of the extracellular matrix.
25347206	4	14	theme	matrix	595:600	arg1	component					564:572	a major component	556:572	a major component of the extracellular matrix	556:600	Hyaluronan is a major component of the extracellular matrix.
25347206	12	15	theme	individuals	1725:1735	arg1	conditions					1711:1720	the pathological conditions	1694:1720	the pathological conditions of individuals	1694:1735	This finding has exciting implications for future research: the molecular composition of a protein corona formed on a nanoparticle surface upon mixing the nanoparticle with blood or other biological fluids may vary according to the pathological conditions of individuals, and the analysis of these compositions could potentially lead to new biomarker discovery with diagnostic applications.
25347206	1	16	theme	gold	185:188	arg1	nanoparticles					190:202	citrate-capped gold nanoparticles	170:202	citrate-capped gold nanoparticles	170:202	In this study, we investigated the interaction between five biorelevant molecules and citrate-capped gold nanoparticles using dynamic light scattering, ζ-potential analysis, UV-vis absorption spectroscopy, and transmission electron microscopy.
25347206	12	17	theme	molecular	1530:1538	arg1	composition					1540:1550	the molecular composition	1526:1550	the molecular composition of a protein corona formed on a nanoparticle surface upon mixing the nanoparticle with blood or other biological fluids	1526:1670	This finding has exciting implications for future research: the molecular composition of a protein corona formed on a nanoparticle surface upon mixing the nanoparticle with blood or other biological fluids may vary according to the pathological conditions of individuals, and the analysis of these compositions could potentially lead to new biomarker discovery with diagnostic applications.
25347206	8	18	theme	rabbit	1026:1031	arg1	IgG					1033:1035	rabbit IgG	1026:1035	rabbit IgG	1026:1035	BSA, rabbit IgG, and hyaluronan formed a stable monolayer on the nanoparticle surface.
25347206	10	19	theme	strong	1338:1343	arg1	affinity					1345:1352	an exceptionally strong affinity	1321:1352	an exceptionally strong affinity toward the citrate-gold nanoparticles	1321:1390	For the first time, we discovered that hyaluronan, a highly negatively charged polyglycosaminoglycan, exhibits an exceptionally strong affinity toward the citrate-gold nanoparticles.
25347206	12	20	theme	nanoparticle	1584:1595	arg1	surface					1597:1603	a nanoparticle surface	1582:1603	a nanoparticle surface	1582:1603	This finding has exciting implications for future research: the molecular composition of a protein corona formed on a nanoparticle surface upon mixing the nanoparticle with blood or other biological fluids may vary according to the pathological conditions of individuals, and the analysis of these compositions could potentially lead to new biomarker discovery with diagnostic applications.
25347206	0	21	theme	interaction	10:20	arg1	modes					22:26	Different interaction modes	0:26	Different interaction modes of biomolecules with citrate-capped gold nanoparticles	0:81	Different interaction modes of biomolecules with citrate-capped gold nanoparticles.
25347206	12	22	theme	biomarker	1807:1815	arg1	discovery					1817:1825	new biomarker discovery	1803:1825	new biomarker discovery with diagnostic applications	1803:1854	This finding has exciting implications for future research: the molecular composition of a protein corona formed on a nanoparticle surface upon mixing the nanoparticle with blood or other biological fluids may vary according to the pathological conditions of individuals, and the analysis of these compositions could potentially lead to new biomarker discovery with diagnostic applications.
25347206	12	23	contain	has	1479:1481	arg1	finding					1471:1477	This finding	1466:1477	This finding	1466:1477	This finding has exciting implications for future research: the molecular composition of a protein corona formed on a nanoparticle surface upon mixing the nanoparticle with blood or other biological fluids may vary according to the pathological conditions of individuals, and the analysis of these compositions could potentially lead to new biomarker discovery with diagnostic applications.
25347206	12	23	contain	has	1479:1481	arg2	implications					1492:1503	exciting implications	1483:1503	exciting implications for future research	1483:1523	This finding has exciting implications for future research: the molecular composition of a protein corona formed on a nanoparticle surface upon mixing the nanoparticle with blood or other biological fluids may vary according to the pathological conditions of individuals, and the analysis of these compositions could potentially lead to new biomarker discovery with diagnostic applications.
25347206	2	24	theme	bovine	354:359	arg1	biomolecules					337:348	The five biomolecules	328:348	The five biomolecules	328:348	The five biomolecules are bovine serum albumin (BSA), two immunoglobulin G (IgG) proteins, immunoglobulin M (IgM), and a polysaccharide molecule, hyaluronan.
25347206	2	24	theme	bovine	354:359	arg1	BSA					376:378	BSA	376:378	BSA	376:378	The five biomolecules are bovine serum albumin (BSA), two immunoglobulin G (IgG) proteins, immunoglobulin M (IgM), and a polysaccharide molecule, hyaluronan.
25347206	2	24	theme	bovine	354:359	arg1	albumin					367:373	bovine serum albumin	354:373	bovine serum albumin (BSA)	354:379	The five biomolecules are bovine serum albumin (BSA), two immunoglobulin G (IgG) proteins, immunoglobulin M (IgM), and a polysaccharide molecule, hyaluronan.
25347206	12	25	with	discovery	1817:1825	arg1	applications					1843:1854	diagnostic applications	1832:1854	diagnostic applications	1832:1854	This finding has exciting implications for future research: the molecular composition of a protein corona formed on a nanoparticle surface upon mixing the nanoparticle with blood or other biological fluids may vary according to the pathological conditions of individuals, and the analysis of these compositions could potentially lead to new biomarker discovery with diagnostic applications.
25347206	0	26	theme	Different	0:8	arg1	modes					22:26	Different interaction modes	0:26	Different interaction modes of biomolecules with citrate-capped gold nanoparticles	0:81	Different interaction modes of biomolecules with citrate-capped gold nanoparticles.
25347206	1	27	theme	electron	307:314	arg1	microscopy					316:325	transmission electron microscopy	294:325	transmission electron microscopy	294:325	In this study, we investigated the interaction between five biorelevant molecules and citrate-capped gold nanoparticles using dynamic light scattering, ζ-potential analysis, UV-vis absorption spectroscopy, and transmission electron microscopy.
25347206	5	28	theme	abnormal	606:613	arg1	level					615:619	An abnormal level	603:619	An abnormal level of hyaluronan in blood	603:642	An abnormal level of hyaluronan in blood is associated with a number of medical conditions including rheumatoid arthritis and malignancy.
25347206	6	29	theme	interaction	756:766	arg1	modes					768:772	Five different interaction modes	741:772	Five different interaction modes	741:772	Five different interaction modes were observed from these molecules.
25347206	2	30	theme	G	401:401	arg1	proteins					409:416	two immunoglobulin G (IgG) proteins	382:416	two immunoglobulin G (IgG) proteins	382:416	The five biomolecules are bovine serum albumin (BSA), two immunoglobulin G (IgG) proteins, immunoglobulin M (IgM), and a polysaccharide molecule, hyaluronan.
25347206	2	30	theme	G	401:401	arg1	M					434:434	immunoglobulin M	419:434	immunoglobulin M (IgM)	419:440	The five biomolecules are bovine serum albumin (BSA), two immunoglobulin G (IgG) proteins, immunoglobulin M (IgM), and a polysaccharide molecule, hyaluronan.
25347206	7	31	theme	citrate	912:918	arg1	ligands					920:926	the citrate ligands	908:926	the citrate ligands	908:926	While BSA and IgM interact with the gold nanoparticles by forming electrostatic interactions with the citrate ligands, IgG and hyaluronan adsorb to the nanoparticle metal core by displacing the citrate ligands.
25347206	6	32	theme	different	746:754	arg1	modes					768:772	Five different interaction modes	741:772	Five different interaction modes	741:772	Five different interaction modes were observed from these molecules.
25347206	10	33	theme	charged	1281:1287	arg1	hyaluronan					1249:1258	hyaluronan	1249:1258	hyaluronan	1249:1258	For the first time, we discovered that hyaluronan, a highly negatively charged polyglycosaminoglycan, exhibits an exceptionally strong affinity toward the citrate-gold nanoparticles.
25347206	10	33	theme	charged	1281:1287	arg1	polyglycosaminoglycan					1289:1309	a highly negatively charged polyglycosaminoglycan	1261:1309	a highly negatively charged polyglycosaminoglycan	1261:1309	For the first time, we discovered that hyaluronan, a highly negatively charged polyglycosaminoglycan, exhibits an exceptionally strong affinity toward the citrate-gold nanoparticles.
25347206	1	34	theme	light	218:222	arg1	scattering					224:233	dynamic light scattering	210:233	dynamic light scattering	210:233	In this study, we investigated the interaction between five biorelevant molecules and citrate-capped gold nanoparticles using dynamic light scattering, ζ-potential analysis, UV-vis absorption spectroscopy, and transmission electron microscopy.
25347206	9	35	theme	gold	1190:1193	arg1	nanoparticles					1195:1207	the gold nanoparticles	1186:1207	the gold nanoparticles	1186:1207	Human IgG and IgM caused nanoparticle cluster formation upon interacting with the gold nanoparticles.
25347206	12	36	theme	diagnostic	1832:1841	arg1	applications					1843:1854	diagnostic applications	1832:1854	diagnostic applications	1832:1854	This finding has exciting implications for future research: the molecular composition of a protein corona formed on a nanoparticle surface upon mixing the nanoparticle with blood or other biological fluids may vary according to the pathological conditions of individuals, and the analysis of these compositions could potentially lead to new biomarker discovery with diagnostic applications.
25347206	10	37	theme	citrate-gold	1365:1376	arg1	nanoparticles					1378:1390	the citrate-gold nanoparticles	1361:1390	the citrate-gold nanoparticles	1361:1390	For the first time, we discovered that hyaluronan, a highly negatively charged polyglycosaminoglycan, exhibits an exceptionally strong affinity toward the citrate-gold nanoparticles.
25347206	2	38	theme	immunoglobulin	419:432	arg1	IgM					437:439	IgM	437:439	IgM	437:439	The five biomolecules are bovine serum albumin (BSA), two immunoglobulin G (IgG) proteins, immunoglobulin M (IgM), and a polysaccharide molecule, hyaluronan.
25347206	2	38	theme	immunoglobulin	419:432	arg1	proteins					409:416	two immunoglobulin G (IgG) proteins	382:416	two immunoglobulin G (IgG) proteins	382:416	The five biomolecules are bovine serum albumin (BSA), two immunoglobulin G (IgG) proteins, immunoglobulin M (IgM), and a polysaccharide molecule, hyaluronan.
25347206	2	38	theme	immunoglobulin	419:432	arg1	M					434:434	immunoglobulin M	419:434	immunoglobulin M (IgM)	419:440	The five biomolecules are bovine serum albumin (BSA), two immunoglobulin G (IgG) proteins, immunoglobulin M (IgM), and a polysaccharide molecule, hyaluronan.
25347206	11	39	theme	gold	1446:1449	arg1	nanoparticles					1451:1463	the gold nanoparticles	1442:1463	the gold nanoparticles	1442:1463	It can effectively compete with IgG to adsorb to the gold nanoparticles.
25347206	12	40	theme	other	1648:1652	arg1	fluids					1665:1670	other biological fluids	1648:1670	other biological fluids	1648:1670	This finding has exciting implications for future research: the molecular composition of a protein corona formed on a nanoparticle surface upon mixing the nanoparticle with blood or other biological fluids may vary according to the pathological conditions of individuals, and the analysis of these compositions could potentially lead to new biomarker discovery with diagnostic applications.
25347206	8	41	theme	stable	1062:1067	arg1	monolayer					1069:1077	a stable monolayer	1060:1077	a stable monolayer	1060:1077	BSA, rabbit IgG, and hyaluronan formed a stable monolayer on the nanoparticle surface.
25347206	5	42	theme	medical	675:681	arg1	conditions					683:692	medical conditions	675:692	medical conditions	675:692	An abnormal level of hyaluronan in blood is associated with a number of medical conditions including rheumatoid arthritis and malignancy.
25347206	1	43	theme	dynamic	210:216	arg1	scattering					224:233	dynamic light scattering	210:233	dynamic light scattering	210:233	In this study, we investigated the interaction between five biorelevant molecules and citrate-capped gold nanoparticles using dynamic light scattering, ζ-potential analysis, UV-vis absorption spectroscopy, and transmission electron microscopy.
25347206	2	44	theme	polysaccharide	449:462	arg1	hyaluronan					474:483	hyaluronan	474:483	hyaluronan	474:483	The five biomolecules are bovine serum albumin (BSA), two immunoglobulin G (IgG) proteins, immunoglobulin M (IgM), and a polysaccharide molecule, hyaluronan.
25347206	2	44	theme	polysaccharide	449:462	arg1	molecule					464:471	a polysaccharide molecule	447:471	a polysaccharide molecule	447:471	The five biomolecules are bovine serum albumin (BSA), two immunoglobulin G (IgG) proteins, immunoglobulin M (IgM), and a polysaccharide molecule, hyaluronan.
25347206	0	45	theme	biomolecules	31:42	arg1	modes					22:26	Different interaction modes	0:26	Different interaction modes of biomolecules with citrate-capped gold nanoparticles	0:81	Different interaction modes of biomolecules with citrate-capped gold nanoparticles.
25347206	4	46	theme	extracellular	581:593	arg1	matrix					595:600	the extracellular matrix	577:600	the extracellular matrix	577:600	Hyaluronan is a major component of the extracellular matrix.
25347206	12	47	theme	compositions	1764:1775	arg1	analysis					1746:1753	the analysis	1742:1753	the analysis of these compositions	1742:1775	This finding has exciting implications for future research: the molecular composition of a protein corona formed on a nanoparticle surface upon mixing the nanoparticle with blood or other biological fluids may vary according to the pathological conditions of individuals, and the analysis of these compositions could potentially lead to new biomarker discovery with diagnostic applications.
25347206	1	48	theme	ζ-potential	236:246	arg1	analysis					248:255	ζ-potential analysis	236:255	ζ-potential analysis	236:255	In this study, we investigated the interaction between five biorelevant molecules and citrate-capped gold nanoparticles using dynamic light scattering, ζ-potential analysis, UV-vis absorption spectroscopy, and transmission electron microscopy.
25347206	0	49	theme	gold	64:67	arg1	nanoparticles					69:81	citrate-capped gold nanoparticles	49:81	citrate-capped gold nanoparticles	49:81	Different interaction modes of biomolecules with citrate-capped gold nanoparticles.
25347206	12	50	theme	future	1509:1514	arg1	research					1516:1523	future research	1509:1523	future research	1509:1523	This finding has exciting implications for future research: the molecular composition of a protein corona formed on a nanoparticle surface upon mixing the nanoparticle with blood or other biological fluids may vary according to the pathological conditions of individuals, and the analysis of these compositions could potentially lead to new biomarker discovery with diagnostic applications.
25347206	0	51	with	modes	22:26	arg1	nanoparticles					69:81	citrate-capped gold nanoparticles	49:81	citrate-capped gold nanoparticles	49:81	Different interaction modes of biomolecules with citrate-capped gold nanoparticles.
25347206	0	52	theme	citrate-capped	49:62	arg1	nanoparticles					69:81	citrate-capped gold nanoparticles	49:81	citrate-capped gold nanoparticles	49:81	Different interaction modes of biomolecules with citrate-capped gold nanoparticles.
25347206	5	53	from	level	615:619	arg1	blood					638:642	blood	638:642	blood	638:642	An abnormal level of hyaluronan in blood is associated with a number of medical conditions including rheumatoid arthritis and malignancy.
25347206	9	54	theme	Human	1108:1112	arg1	IgG					1114:1116	IgG	1114:1116	IgG	1114:1116	Human IgG and IgM caused nanoparticle cluster formation upon interacting with the gold nanoparticles.
25347206	3	55	from	proteins	523:530	arg1	blood					535:539	blood	535:539	blood	535:539	BSA, IgG, and IgM are high abundance proteins in blood.
25347206	8	56	theme	nanoparticle	1086:1097	arg1	surface					1099:1105	the nanoparticle surface	1082:1105	the nanoparticle surface	1082:1105	BSA, rabbit IgG, and hyaluronan formed a stable monolayer on the nanoparticle surface.
25347206	1	57	theme	biorelevant	144:154	arg1	molecules					156:164	five biorelevant molecules	139:164	five biorelevant molecules	139:164	In this study, we investigated the interaction between five biorelevant molecules and citrate-capped gold nanoparticles using dynamic light scattering, ζ-potential analysis, UV-vis absorption spectroscopy, and transmission electron microscopy.
25347206	12	58	theme	pathological	1698:1709	arg1	conditions					1711:1720	the pathological conditions	1694:1720	the pathological conditions of individuals	1694:1735	This finding has exciting implications for future research: the molecular composition of a protein corona formed on a nanoparticle surface upon mixing the nanoparticle with blood or other biological fluids may vary according to the pathological conditions of individuals, and the analysis of these compositions could potentially lead to new biomarker discovery with diagnostic applications.
25347206	12	59	theme	exciting	1483:1490	arg1	implications					1492:1503	exciting implications	1483:1503	exciting implications for future research	1483:1523	This finding has exciting implications for future research: the molecular composition of a protein corona formed on a nanoparticle surface upon mixing the nanoparticle with blood or other biological fluids may vary according to the pathological conditions of individuals, and the analysis of these compositions could potentially lead to new biomarker discovery with diagnostic applications.
25347206	7	60	theme	metal	975:979	arg1	core					981:984	the nanoparticle metal core	958:984	the nanoparticle metal core	958:984	While BSA and IgM interact with the gold nanoparticles by forming electrostatic interactions with the citrate ligands, IgG and hyaluronan adsorb to the nanoparticle metal core by displacing the citrate ligands.
25347206	2	61	theme	serum	361:365	arg1	biomolecules					337:348	The five biomolecules	328:348	The five biomolecules	328:348	The five biomolecules are bovine serum albumin (BSA), two immunoglobulin G (IgG) proteins, immunoglobulin M (IgM), and a polysaccharide molecule, hyaluronan.
25347206	2	61	theme	serum	361:365	arg1	BSA					376:378	BSA	376:378	BSA	376:378	The five biomolecules are bovine serum albumin (BSA), two immunoglobulin G (IgG) proteins, immunoglobulin M (IgM), and a polysaccharide molecule, hyaluronan.
25347206	2	61	theme	serum	361:365	arg1	albumin					367:373	bovine serum albumin	354:373	bovine serum albumin (BSA)	354:379	The five biomolecules are bovine serum albumin (BSA), two immunoglobulin G (IgG) proteins, immunoglobulin M (IgM), and a polysaccharide molecule, hyaluronan.
25347206	7	62	theme	electrostatic	876:888	arg1	interactions					890:901	electrostatic interactions	876:901	electrostatic interactions	876:901	While BSA and IgM interact with the gold nanoparticles by forming electrostatic interactions with the citrate ligands, IgG and hyaluronan adsorb to the nanoparticle metal core by displacing the citrate ligands.
25347206	12	63	theme	corona	1565:1570	arg1	composition					1540:1550	the molecular composition	1526:1550	the molecular composition of a protein corona formed on a nanoparticle surface upon mixing the nanoparticle with blood or other biological fluids	1526:1670	This finding has exciting implications for future research: the molecular composition of a protein corona formed on a nanoparticle surface upon mixing the nanoparticle with blood or other biological fluids may vary according to the pathological conditions of individuals, and the analysis of these compositions could potentially lead to new biomarker discovery with diagnostic applications.
25347206	10	64	theme	first	1218:1222	arg1	time					1224:1227	the first time	1214:1227	the first time	1214:1227	For the first time, we discovered that hyaluronan, a highly negatively charged polyglycosaminoglycan, exhibits an exceptionally strong affinity toward the citrate-gold nanoparticles.
25347206	2	65	theme	immunoglobulin	386:399	arg1	IgG					404:406	IgG	404:406	IgG	404:406	The five biomolecules are bovine serum albumin (BSA), two immunoglobulin G (IgG) proteins, immunoglobulin M (IgM), and a polysaccharide molecule, hyaluronan.
25347206	2	65	theme	immunoglobulin	386:399	arg1	G					401:401	immunoglobulin G	386:401	two immunoglobulin G (IgG) proteins	382:416	The five biomolecules are bovine serum albumin (BSA), two immunoglobulin G (IgG) proteins, immunoglobulin M (IgM), and a polysaccharide molecule, hyaluronan.
25347206	1	66	theme	UV-vis	258:263	arg1	spectroscopy					276:287	UV-vis absorption spectroscopy	258:287	UV-vis absorption spectroscopy	258:287	In this study, we investigated the interaction between five biorelevant molecules and citrate-capped gold nanoparticles using dynamic light scattering, ζ-potential analysis, UV-vis absorption spectroscopy, and transmission electron microscopy.
25347206	12	67	theme	protein	1557:1563	arg1	corona					1565:1570	a protein corona	1555:1570	a protein corona formed on a nanoparticle surface upon mixing the nanoparticle with blood or other biological fluids	1555:1670	This finding has exciting implications for future research: the molecular composition of a protein corona formed on a nanoparticle surface upon mixing the nanoparticle with blood or other biological fluids may vary according to the pathological conditions of individuals, and the analysis of these compositions could potentially lead to new biomarker discovery with diagnostic applications.
25347206	12	68	theme	new	1803:1805	arg1	discovery					1817:1825	new biomarker discovery	1803:1825	new biomarker discovery with diagnostic applications	1803:1854	This finding has exciting implications for future research: the molecular composition of a protein corona formed on a nanoparticle surface upon mixing the nanoparticle with blood or other biological fluids may vary according to the pathological conditions of individuals, and the analysis of these compositions could potentially lead to new biomarker discovery with diagnostic applications.
25347206	1	69	theme	absorption	265:274	arg1	spectroscopy					276:287	UV-vis absorption spectroscopy	258:287	UV-vis absorption spectroscopy	258:287	In this study, we investigated the interaction between five biorelevant molecules and citrate-capped gold nanoparticles using dynamic light scattering, ζ-potential analysis, UV-vis absorption spectroscopy, and transmission electron microscopy.
24502924	0	0	theme	arid	86:89	arg1	applications					96:107	arid soil applications	86:107	arid soil applications	86:107	ZnO nanoparticles induced exopolysaccharide production by B. subtilis strain JCT1 for arid soil applications.
24502924	4	1	theme	elemental	604:612	arg1	proportion					614:623	elemental proportion	604:623	elemental proportion	604:623	Putative characterization techniques were employed for confirmation of size, shape, surface structure, crystalline nature and elemental proportion of ZnO nanoparticles.
24502924	3	2	theme	investigations	308:321	arg1	series					298:303	the series	294:303	the series of investigations	294:321	In the series of investigations, environmentally benign protocol was followed for the synthesis of ZnO nanoparticles using extracellular enzymes obtained from Aspergillus fumigatus TFR8.
24502924	4	3	theme	size	549:552	arg1	confirmation					533:544	confirmation	533:544	confirmation of size, shape, surface structure, crystalline nature and elemental proportion of ZnO nanoparticles	533:644	Putative characterization techniques were employed for confirmation of size, shape, surface structure, crystalline nature and elemental proportion of ZnO nanoparticles.
24502924	5	4	theme	ZnO	686:688	arg1	nanoparticles					690:702	ZnO nanoparticles	686:702	ZnO nanoparticles	686:702	Results established an average size of ZnO nanoparticles to be 2.9 nm at least at one dimension and oblate spherical in structure.
24502924	6	5	theme	Zn	843:844	arg1	percentage					859:868	97.5% Zn element atom percentage	837:868	97.5% Zn element atom percentage	837:868	The qualitative composition of the nanoparticles exhibited 97.5% Zn element atom percentage.
24502924	9	6	theme	mediated	1229:1236	arg1	mechanism					1249:1257	A possible ZnO nanoparticle mediated biological mechanism	1201:1257	A possible ZnO nanoparticle mediated biological mechanism for enhancing exopolysaccharide production	1201:1300	A possible ZnO nanoparticle mediated biological mechanism for enhancing exopolysaccharide production has been discussed.
24502924	7	7	theme	exopolysaccharide	913:929	arg1	production					931:940	exopolysaccharide production	913:940	exopolysaccharide production	913:940	Biosynthesized ZnO nanoparticles enhanced exopolysaccharide production by 596.1% as compared to control and further EPS amelioration led to enhanced soil aggregation (up to 82%), moisture retention (10.7-14.2%) and soil organic carbon.
24502924	5	8	from	dimension	733:741	arg1	nm					714:715	2.9 nm	710:715	2.9 nm	710:715	Results established an average size of ZnO nanoparticles to be 2.9 nm at least at one dimension and oblate spherical in structure.
24502924	5	8	from	dimension	733:741	arg1	structure					767:775	structure	767:775	structure	767:775	Results established an average size of ZnO nanoparticles to be 2.9 nm at least at one dimension and oblate spherical in structure.
24502924	8	9	theme	infra-red	1177:1185	arg1	spectroscopy					1187:1198	infra-red spectroscopy	1177:1198	infra-red spectroscopy	1177:1198	Soil aggregation stability was further confirmed by Fourier transform infra-red spectroscopy.
24502924	9	10	theme	biological	1238:1247	arg1	mechanism					1249:1257	A possible ZnO nanoparticle mediated biological mechanism	1201:1257	A possible ZnO nanoparticle mediated biological mechanism for enhancing exopolysaccharide production	1201:1300	A possible ZnO nanoparticle mediated biological mechanism for enhancing exopolysaccharide production has been discussed.
24502924	5	11	theme	nanoparticles	690:702	arg1	size					678:681	an average size	667:681	an average size of ZnO nanoparticles	667:702	Results established an average size of ZnO nanoparticles to be 2.9 nm at least at one dimension and oblate spherical in structure.
24502924	6	12	theme	%	841:841	arg1	percentage					859:868	97.5% Zn element atom percentage	837:868	97.5% Zn element atom percentage	837:868	The qualitative composition of the nanoparticles exhibited 97.5% Zn element atom percentage.
24502924	0	13	theme	soil	91:94	arg1	applications					96:107	arid soil applications	86:107	arid soil applications	86:107	ZnO nanoparticles induced exopolysaccharide production by B. subtilis strain JCT1 for arid soil applications.
24502924	6	14	theme	97.5	837:840	arg1	%					841:841	%	841:841	%	841:841	The qualitative composition of the nanoparticles exhibited 97.5% Zn element atom percentage.
24502924	2	15	theme	soil	272:275	arg1	applications					277:288	arid soil applications	267:288	arid soil applications	267:288	JN194187) is a novel approach for arid soil applications.
24502924	4	16	theme	proportion	614:623	arg1	confirmation					533:544	confirmation	533:544	confirmation of size, shape, surface structure, crystalline nature and elemental proportion of ZnO nanoparticles	533:644	Putative characterization techniques were employed for confirmation of size, shape, surface structure, crystalline nature and elemental proportion of ZnO nanoparticles.
24502924	3	17	theme	benign	340:345	arg1	protocol					347:354	environmentally benign protocol	324:354	environmentally benign protocol	324:354	In the series of investigations, environmentally benign protocol was followed for the synthesis of ZnO nanoparticles using extracellular enzymes obtained from Aspergillus fumigatus TFR8.
24502924	4	18	theme	characterization	487:502	arg1	techniques					504:513	Putative characterization techniques	478:513	Putative characterization techniques	478:513	Putative characterization techniques were employed for confirmation of size, shape, surface structure, crystalline nature and elemental proportion of ZnO nanoparticles.
24502924	8	19	dep	Fourier	1159:1165	arg1	transform					1167:1175	transform	1167:1175	transform infra-red spectroscopy	1167:1198	Soil aggregation stability was further confirmed by Fourier transform infra-red spectroscopy.
24502924	9	20	theme	nanoparticle	1216:1227	arg1	mechanism					1249:1257	A possible ZnO nanoparticle mediated biological mechanism	1201:1257	A possible ZnO nanoparticle mediated biological mechanism for enhancing exopolysaccharide production	1201:1300	A possible ZnO nanoparticle mediated biological mechanism for enhancing exopolysaccharide production has been discussed.
24502924	2	21	theme	arid	267:270	arg1	applications					277:288	arid soil applications	267:288	arid soil applications	267:288	JN194187) is a novel approach for arid soil applications.
24502924	7	22	dep	82	1044:1045	arg1	to					1041:1042	to	1041:1042	to	1041:1042	Biosynthesized ZnO nanoparticles enhanced exopolysaccharide production by 596.1% as compared to control and further EPS amelioration led to enhanced soil aggregation (up to 82%), moisture retention (10.7-14.2%) and soil organic carbon.
24502924	1	23	theme	Bacillus	175:182	arg1	JCT1					200:203	Bacillus subtilis strain JCT1	175:203	Bacillus subtilis strain JCT1	175:203	ZnO nanoparticle induced exopolysaccharide (EPS) production from Bacillus subtilis strain JCT1 (NCBI GenBank Accession No.
24502924	0	24	theme	B.	58:59	arg1	JCT1					77:80	B. subtilis strain JCT1	58:80	B. subtilis strain JCT1 for arid soil applications	58:107	ZnO nanoparticles induced exopolysaccharide production by B. subtilis strain JCT1 for arid soil applications.
24502924	9	25	theme	ZnO	1212:1214	arg1	mechanism					1249:1257	A possible ZnO nanoparticle mediated biological mechanism	1201:1257	A possible ZnO nanoparticle mediated biological mechanism for enhancing exopolysaccharide production	1201:1300	A possible ZnO nanoparticle mediated biological mechanism for enhancing exopolysaccharide production has been discussed.
24502924	6	26	theme	nanoparticles	813:825	arg1	composition					794:804	The qualitative composition	778:804	The qualitative composition of the nanoparticles	778:825	The qualitative composition of the nanoparticles exhibited 97.5% Zn element atom percentage.
24502924	1	27	dep	induced	127:133	arg1	GenBank					211:217	GenBank	211:217	GenBank	211:217	ZnO nanoparticle induced exopolysaccharide (EPS) production from Bacillus subtilis strain JCT1 (NCBI GenBank Accession No.
24502924	7	28	theme	Biosynthesized	871:884	arg1	nanoparticles					890:902	Biosynthesized ZnO nanoparticles	871:902	Biosynthesized ZnO nanoparticles	871:902	Biosynthesized ZnO nanoparticles enhanced exopolysaccharide production by 596.1% as compared to control and further EPS amelioration led to enhanced soil aggregation (up to 82%), moisture retention (10.7-14.2%) and soil organic carbon.
24502924	3	29	theme	Aspergillus	450:460	arg1	TFR8					472:475	Aspergillus fumigatus TFR8	450:475	Aspergillus fumigatus TFR8	450:475	In the series of investigations, environmentally benign protocol was followed for the synthesis of ZnO nanoparticles using extracellular enzymes obtained from Aspergillus fumigatus TFR8.
24502924	5	30	theme	average	670:676	arg1	size					678:681	an average size	667:681	an average size of ZnO nanoparticles	667:702	Results established an average size of ZnO nanoparticles to be 2.9 nm at least at one dimension and oblate spherical in structure.
24502924	7	31	theme	EPS	987:989	arg1	amelioration					991:1002	further EPS amelioration	979:1002	further EPS amelioration	979:1002	Biosynthesized ZnO nanoparticles enhanced exopolysaccharide production by 596.1% as compared to control and further EPS amelioration led to enhanced soil aggregation (up to 82%), moisture retention (10.7-14.2%) and soil organic carbon.
24502924	7	32	theme	ZnO	886:888	arg1	nanoparticles					890:902	Biosynthesized ZnO nanoparticles	871:902	Biosynthesized ZnO nanoparticles	871:902	Biosynthesized ZnO nanoparticles enhanced exopolysaccharide production by 596.1% as compared to control and further EPS amelioration led to enhanced soil aggregation (up to 82%), moisture retention (10.7-14.2%) and soil organic carbon.
24502924	1	33	theme	strain	193:198	arg1	JCT1					200:203	Bacillus subtilis strain JCT1	175:203	Bacillus subtilis strain JCT1	175:203	ZnO nanoparticle induced exopolysaccharide (EPS) production from Bacillus subtilis strain JCT1 (NCBI GenBank Accession No.
24502924	0	34	theme	ZnO	0:2	arg1	nanoparticles					4:16	ZnO nanoparticles	0:16	ZnO nanoparticles	0:16	ZnO nanoparticles induced exopolysaccharide production by B. subtilis strain JCT1 for arid soil applications.
24502924	7	35	theme	organic	1091:1097	arg1	carbon					1099:1104	soil organic carbon	1086:1104	soil organic carbon	1086:1104	Biosynthesized ZnO nanoparticles enhanced exopolysaccharide production by 596.1% as compared to control and further EPS amelioration led to enhanced soil aggregation (up to 82%), moisture retention (10.7-14.2%) and soil organic carbon.
24502924	0	36	theme	exopolysaccharide	26:42	arg1	production					44:53	exopolysaccharide production	26:53	exopolysaccharide production	26:53	ZnO nanoparticles induced exopolysaccharide production by B. subtilis strain JCT1 for arid soil applications.
24502924	4	37	theme	structure	570:578	arg1	confirmation					533:544	confirmation	533:544	confirmation of size, shape, surface structure, crystalline nature and elemental proportion of ZnO nanoparticles	533:644	Putative characterization techniques were employed for confirmation of size, shape, surface structure, crystalline nature and elemental proportion of ZnO nanoparticles.
24502924	9	38	theme	possible	1203:1210	arg1	mechanism					1249:1257	A possible ZnO nanoparticle mediated biological mechanism	1201:1257	A possible ZnO nanoparticle mediated biological mechanism for enhancing exopolysaccharide production	1201:1300	A possible ZnO nanoparticle mediated biological mechanism for enhancing exopolysaccharide production has been discussed.
24502924	4	39	theme	nature	593:598	arg1	confirmation					533:544	confirmation	533:544	confirmation of size, shape, surface structure, crystalline nature and elemental proportion of ZnO nanoparticles	533:644	Putative characterization techniques were employed for confirmation of size, shape, surface structure, crystalline nature and elemental proportion of ZnO nanoparticles.
24502924	5	40	from	spherical	754:762	arg1	nm					714:715	2.9 nm	710:715	2.9 nm	710:715	Results established an average size of ZnO nanoparticles to be 2.9 nm at least at one dimension and oblate spherical in structure.
24502924	5	40	from	spherical	754:762	arg1	structure					767:775	structure	767:775	structure	767:775	Results established an average size of ZnO nanoparticles to be 2.9 nm at least at one dimension and oblate spherical in structure.
24502924	1	41	theme	ZnO	110:112	arg1	nanoparticle					114:125	ZnO nanoparticle	110:125	ZnO nanoparticle	110:125	ZnO nanoparticle induced exopolysaccharide (EPS) production from Bacillus subtilis strain JCT1 (NCBI GenBank Accession No.
24502924	4	42	theme	crystalline	581:591	arg1	nature					593:598	crystalline nature	581:598	crystalline nature	581:598	Putative characterization techniques were employed for confirmation of size, shape, surface structure, crystalline nature and elemental proportion of ZnO nanoparticles.
24502924	9	43	theme	exopolysaccharide	1273:1289	arg1	production					1291:1300	exopolysaccharide production	1273:1300	exopolysaccharide production	1273:1300	A possible ZnO nanoparticle mediated biological mechanism for enhancing exopolysaccharide production has been discussed.
24502924	3	44	theme	extracellular	414:426	arg1	enzymes					428:434	extracellular enzymes	414:434	extracellular enzymes obtained from Aspergillus fumigatus TFR8	414:475	In the series of investigations, environmentally benign protocol was followed for the synthesis of ZnO nanoparticles using extracellular enzymes obtained from Aspergillus fumigatus TFR8.
24502924	4	45	theme	shape	555:559	arg1	confirmation					533:544	confirmation	533:544	confirmation of size, shape, surface structure, crystalline nature and elemental proportion of ZnO nanoparticles	533:644	Putative characterization techniques were employed for confirmation of size, shape, surface structure, crystalline nature and elemental proportion of ZnO nanoparticles.
24502924	4	46	theme	Putative	478:485	arg1	techniques					504:513	Putative characterization techniques	478:513	Putative characterization techniques	478:513	Putative characterization techniques were employed for confirmation of size, shape, surface structure, crystalline nature and elemental proportion of ZnO nanoparticles.
24502924	0	47	dep	B.	58:59	arg1	subtilis					61:68	subtilis	61:68	subtilis	61:68	ZnO nanoparticles induced exopolysaccharide production by B. subtilis strain JCT1 for arid soil applications.
24502924	6	48	theme	qualitative	782:792	arg1	composition					794:804	The qualitative composition	778:804	The qualitative composition of the nanoparticles	778:825	The qualitative composition of the nanoparticles exhibited 97.5% Zn element atom percentage.
24502924	1	49	theme	Accession	219:227	arg1	No					229:230	Accession No	219:230	NCBI GenBank Accession No	206:230	ZnO nanoparticle induced exopolysaccharide (EPS) production from Bacillus subtilis strain JCT1 (NCBI GenBank Accession No.
24502924	3	50	theme	ZnO	390:392	arg1	nanoparticles					394:406	ZnO nanoparticles	390:406	ZnO nanoparticles using extracellular enzymes obtained from Aspergillus fumigatus TFR8	390:475	In the series of investigations, environmentally benign protocol was followed for the synthesis of ZnO nanoparticles using extracellular enzymes obtained from Aspergillus fumigatus TFR8.
24502924	8	51	theme	aggregation	1112:1122	arg1	stability					1124:1132	Soil aggregation stability	1107:1132	Soil aggregation stability	1107:1132	Soil aggregation stability was further confirmed by Fourier transform infra-red spectroscopy.
24502924	7	52	theme	soil	1086:1089	arg1	carbon					1099:1104	soil organic carbon	1086:1104	soil organic carbon	1086:1104	Biosynthesized ZnO nanoparticles enhanced exopolysaccharide production by 596.1% as compared to control and further EPS amelioration led to enhanced soil aggregation (up to 82%), moisture retention (10.7-14.2%) and soil organic carbon.
24502924	1	53	dep	Bacillus	175:182	arg1	subtilis					184:191	subtilis	184:191	subtilis	184:191	ZnO nanoparticle induced exopolysaccharide (EPS) production from Bacillus subtilis strain JCT1 (NCBI GenBank Accession No.
24502924	3	54	theme	nanoparticles	394:406	arg1	synthesis					377:385	the synthesis	373:385	the synthesis of ZnO nanoparticles using extracellular enzymes obtained from Aspergillus fumigatus TFR8	373:475	In the series of investigations, environmentally benign protocol was followed for the synthesis of ZnO nanoparticles using extracellular enzymes obtained from Aspergillus fumigatus TFR8.
24502924	4	55	theme	surface	562:568	arg1	structure					570:578	surface structure	562:578	surface structure	562:578	Putative characterization techniques were employed for confirmation of size, shape, surface structure, crystalline nature and elemental proportion of ZnO nanoparticles.
24502924	8	56	theme	Soil	1107:1110	arg1	stability					1124:1132	Soil aggregation stability	1107:1132	Soil aggregation stability	1107:1132	Soil aggregation stability was further confirmed by Fourier transform infra-red spectroscopy.
24502924	3	57	dep	Aspergillus	450:460	arg1	fumigatus					462:470	fumigatus	462:470	fumigatus	462:470	In the series of investigations, environmentally benign protocol was followed for the synthesis of ZnO nanoparticles using extracellular enzymes obtained from Aspergillus fumigatus TFR8.
24502924	7	58	theme	further	979:985	arg1	amelioration					991:1002	further EPS amelioration	979:1002	further EPS amelioration	979:1002	Biosynthesized ZnO nanoparticles enhanced exopolysaccharide production by 596.1% as compared to control and further EPS amelioration led to enhanced soil aggregation (up to 82%), moisture retention (10.7-14.2%) and soil organic carbon.
24502924	1	59	dep	GenBank	211:217	arg1	No					229:230	Accession No	219:230	NCBI GenBank Accession No	206:230	ZnO nanoparticle induced exopolysaccharide (EPS) production from Bacillus subtilis strain JCT1 (NCBI GenBank Accession No.
24502924	7	60	theme	enhanced	1011:1018	arg1	%					1046:1046	up to 82%	1038:1046	up to 82%	1038:1046	Biosynthesized ZnO nanoparticles enhanced exopolysaccharide production by 596.1% as compared to control and further EPS amelioration led to enhanced soil aggregation (up to 82%), moisture retention (10.7-14.2%) and soil organic carbon.
24502924	7	60	theme	enhanced	1011:1018	arg1	aggregation					1025:1035	enhanced soil aggregation	1011:1035	enhanced soil aggregation (up to 82%)	1011:1047	Biosynthesized ZnO nanoparticles enhanced exopolysaccharide production by 596.1% as compared to control and further EPS amelioration led to enhanced soil aggregation (up to 82%), moisture retention (10.7-14.2%) and soil organic carbon.
24502924	1	61	theme	exopolysaccharide	135:151	arg1	production					159:168	exopolysaccharide (EPS) production	135:168	exopolysaccharide (EPS) production from Bacillus subtilis strain JCT1	135:203	ZnO nanoparticle induced exopolysaccharide (EPS) production from Bacillus subtilis strain JCT1 (NCBI GenBank Accession No.
24502924	4	62	theme	nanoparticles	632:644	arg1	proportion					614:623	elemental proportion	604:623	elemental proportion	604:623	Putative characterization techniques were employed for confirmation of size, shape, surface structure, crystalline nature and elemental proportion of ZnO nanoparticles.
24502924	4	62	theme	nanoparticles	632:644	arg1	nature					593:598	crystalline nature	581:598	crystalline nature	581:598	Putative characterization techniques were employed for confirmation of size, shape, surface structure, crystalline nature and elemental proportion of ZnO nanoparticles.
24502924	4	62	theme	nanoparticles	632:644	arg1	shape					555:559	shape	555:559	shape	555:559	Putative characterization techniques were employed for confirmation of size, shape, surface structure, crystalline nature and elemental proportion of ZnO nanoparticles.
24502924	4	62	theme	nanoparticles	632:644	arg1	structure					570:578	surface structure	562:578	surface structure	562:578	Putative characterization techniques were employed for confirmation of size, shape, surface structure, crystalline nature and elemental proportion of ZnO nanoparticles.
24502924	4	62	theme	nanoparticles	632:644	arg1	size					549:552	size	549:552	size	549:552	Putative characterization techniques were employed for confirmation of size, shape, surface structure, crystalline nature and elemental proportion of ZnO nanoparticles.
24502924	7	63	theme	moisture	1050:1057	arg1	retention					1059:1067	moisture retention	1050:1067	moisture retention (10.7-14.2%)	1050:1080	Biosynthesized ZnO nanoparticles enhanced exopolysaccharide production by 596.1% as compared to control and further EPS amelioration led to enhanced soil aggregation (up to 82%), moisture retention (10.7-14.2%) and soil organic carbon.
24502924	7	63	theme	moisture	1050:1057	arg1	%					1079:1079	10.7-14.2%	1070:1079	10.7-14.2%	1070:1079	Biosynthesized ZnO nanoparticles enhanced exopolysaccharide production by 596.1% as compared to control and further EPS amelioration led to enhanced soil aggregation (up to 82%), moisture retention (10.7-14.2%) and soil organic carbon.
24502924	6	64	theme	atom	854:857	arg1	percentage					859:868	97.5% Zn element atom percentage	837:868	97.5% Zn element atom percentage	837:868	The qualitative composition of the nanoparticles exhibited 97.5% Zn element atom percentage.
24502924	2	65	theme	novel	248:252	arg1	JN194187					233:240	JN194187	233:240	JN194187	233:240	JN194187) is a novel approach for arid soil applications.
24502924	2	65	theme	novel	248:252	arg1	approach					254:261	a novel approach	246:261	a novel approach for arid soil applications	246:288	JN194187) is a novel approach for arid soil applications.
24502924	4	66	theme	ZnO	628:630	arg1	nanoparticles					632:644	ZnO nanoparticles	628:644	ZnO nanoparticles	628:644	Putative characterization techniques were employed for confirmation of size, shape, surface structure, crystalline nature and elemental proportion of ZnO nanoparticles.
24502924	6	67	theme	element	846:852	arg1	percentage					859:868	97.5% Zn element atom percentage	837:868	97.5% Zn element atom percentage	837:868	The qualitative composition of the nanoparticles exhibited 97.5% Zn element atom percentage.
24502924	1	68	from	JCT1	200:203	arg1	production					159:168	exopolysaccharide (EPS) production	135:168	exopolysaccharide (EPS) production from Bacillus subtilis strain JCT1	135:203	ZnO nanoparticle induced exopolysaccharide (EPS) production from Bacillus subtilis strain JCT1 (NCBI GenBank Accession No.
24502924	0	69	theme	strain	70:75	arg1	JCT1					77:80	B. subtilis strain JCT1	58:80	B. subtilis strain JCT1 for arid soil applications	58:107	ZnO nanoparticles induced exopolysaccharide production by B. subtilis strain JCT1 for arid soil applications.
24502924	7	70	theme	soil	1020:1023	arg1	%					1046:1046	up to 82%	1038:1046	up to 82%	1038:1046	Biosynthesized ZnO nanoparticles enhanced exopolysaccharide production by 596.1% as compared to control and further EPS amelioration led to enhanced soil aggregation (up to 82%), moisture retention (10.7-14.2%) and soil organic carbon.
24502924	7	70	theme	soil	1020:1023	arg1	aggregation					1025:1035	enhanced soil aggregation	1011:1035	enhanced soil aggregation (up to 82%)	1011:1047	Biosynthesized ZnO nanoparticles enhanced exopolysaccharide production by 596.1% as compared to control and further EPS amelioration led to enhanced soil aggregation (up to 82%), moisture retention (10.7-14.2%) and soil organic carbon.
24502924	5	71	theme	oblate	747:752	arg1	spherical					754:762	oblate spherical	747:762	oblate spherical	747:762	Results established an average size of ZnO nanoparticles to be 2.9 nm at least at one dimension and oblate spherical in structure.
27798741	8	0	theme	leaf	1599:1602	arg1	composition					1619:1629	leaf cellulose δ18O composition	1599:1629	leaf cellulose δ18O composition	1599:1629	Our finding supports a wider applicability of leaf cellulose δ18O composition as a climate proxy for atmospheric humidity conditions during the leaf growing season than previously documented.
27798741	8	1	theme	growing	1702:1708	arg1	season					1710:1715	the leaf growing season than previously documented	1693:1742	the leaf growing season than previously documented	1693:1742	Our finding supports a wider applicability of leaf cellulose δ18O composition as a climate proxy for atmospheric humidity conditions during the leaf growing season than previously documented.
27798741	3	2	theme	north-eastern	668:680	arg1	gradient					701:708	a north-eastern Australian aridity gradient	666:708	a north-eastern Australian aridity gradient	666:708	To progress this aim we measured δ18O and δ2H values in eucalypt leaf and stem water and δ18O values in leaf cellulose, along with the isotopic compositions of water vapour, across a north-eastern Australian aridity gradient.
27798741	2	3	theme	environmental	362:374	arg1	drivers					376:382	both physiological and environmental drivers	339:382	both physiological and environmental drivers of the variations in isotopic enrichments that occur in leaf water and associated organic compounds	339:482	However, accurate interpretation relies on a detailed understanding of both physiological and environmental drivers of the variations in isotopic enrichments that occur in leaf water and associated organic compounds.
27798741	1	4	theme	palaeoclimate	253:265	arg1	indicators					239:248	indicators	239:248	indicators of palaeoclimate	239:265	Several previous studies have investigated the use of the stable hydrogen and oxygen isotope compositions in plant materials as indicators of palaeoclimate.
27798741	1	4	theme	palaeoclimate	253:265	arg1	use					158:160	the use	154:160	the use of the stable hydrogen and oxygen isotope compositions in plant materials	154:234	Several previous studies have investigated the use of the stable hydrogen and oxygen isotope compositions in plant materials as indicators of palaeoclimate.
27798741	4	5	theme	water	894:898	arg1	enrichment					909:918	leaf water isotopic enrichment	889:918	leaf water isotopic enrichment	889:918	Here we compare observed leaf water enrichment, along with previously published enrichment data from a similar north Australian transect, to Craig-Gordon-modelled predictions of leaf water isotopic enrichment.
27798741	7	6	theme	leaf	1497:1500	arg1	values					1517:1522	the observed leaf cellulose δ18O values	1484:1522	the observed leaf cellulose δ18O values	1484:1522	The atmospheric-leaf vapour pressure relationship is also a strong predictor of the observed leaf cellulose δ18O values across one aridity gradient.
27798741	6	7	theme	strong	1302:1307	arg1	deviations					1309:1318	strong deviations	1302:1318	strong deviations from Craig-Gordon predicted isotope enrichments caused by partial stomatal closure	1302:1401	During exceptionally dry and hot conditions (RH < 21%, T > 37 °C) we observed strong deviations from Craig-Gordon predicted isotope enrichments caused by partial stomatal closure.
27798741	3	8	theme	δ18O	518:521	arg1	values					531:536	δ18O and δ2H values	518:536	δ18O and δ2H values in eucalypt leaf and stem water	518:568	To progress this aim we measured δ18O and δ2H values in eucalypt leaf and stem water and δ18O values in leaf cellulose, along with the isotopic compositions of water vapour, across a north-eastern Australian aridity gradient.
27798741	3	9	theme	eucalypt	541:548	arg1	leaf					550:553	eucalypt leaf	541:553	eucalypt leaf	541:553	To progress this aim we measured δ18O and δ2H values in eucalypt leaf and stem water and δ18O values in leaf cellulose, along with the isotopic compositions of water vapour, across a north-eastern Australian aridity gradient.
27798741	1	10	theme	stable	169:174	arg1	hydrogen					176:183	the stable hydrogen and oxygen isotope compositions	165:215	hydrogen	176:183	Several previous studies have investigated the use of the stable hydrogen and oxygen isotope compositions in plant materials as indicators of palaeoclimate.
27798741	7	11	theme	values	1517:1522	arg1	predictor					1471:1479	a strong predictor	1462:1479	a strong predictor of the observed leaf cellulose δ18O values across one aridity gradient	1462:1550	The atmospheric-leaf vapour pressure relationship is also a strong predictor of the observed leaf cellulose δ18O values across one aridity gradient.
27798741	7	11	theme	values	1517:1522	arg1	relationship					1441:1452	The atmospheric-leaf vapour pressure relationship	1404:1452	The atmospheric-leaf vapour pressure relationship	1404:1452	The atmospheric-leaf vapour pressure relationship is also a strong predictor of the observed leaf cellulose δ18O values across one aridity gradient.
27798741	4	12	theme	Craig-Gordon-modelled	852:872	arg1	predictions					874:884	Craig-Gordon-modelled predictions	852:884	Craig-Gordon-modelled predictions of leaf water isotopic enrichment	852:918	Here we compare observed leaf water enrichment, along with previously published enrichment data from a similar north Australian transect, to Craig-Gordon-modelled predictions of leaf water isotopic enrichment.
27798741	2	13	theme	detailed	313:320	arg1	understanding					322:334	a detailed understanding	311:334	a detailed understanding of both physiological and environmental drivers of the variations in isotopic enrichments that occur in leaf water and associated organic compounds	311:482	However, accurate interpretation relies on a detailed understanding of both physiological and environmental drivers of the variations in isotopic enrichments that occur in leaf water and associated organic compounds.
27798741	6	14	theme	stomatal	1386:1393	arg1	closure					1395:1401	partial stomatal closure	1378:1401	partial stomatal closure	1378:1401	During exceptionally dry and hot conditions (RH < 21%, T > 37 °C) we observed strong deviations from Craig-Gordon predicted isotope enrichments caused by partial stomatal closure.
27798741	5	15	theme	vapour	1100:1105	arg1	pressure					1107:1114	atmospheric and internal leaf water vapour pressure	1064:1114	pressure	1107:1114	Our investigation of model parameters shows that observed 18O enrichment across the aridity gradients is dominated by the relationship between atmospheric and internal leaf water vapour pressure while 2H enrichment is driven mainly by variation in the water vapour-source water isotopic disequilibrium.
27798741	1	16	theme	isotope	196:202	arg1	compositions					204:215	the stable hydrogen and oxygen isotope compositions	165:215	compositions	204:215	Several previous studies have investigated the use of the stable hydrogen and oxygen isotope compositions in plant materials as indicators of palaeoclimate.
27798741	4	17	from	Australian	828:837	arg1	data					802:805	previously published enrichment data	770:805	previously published enrichment data from a similar north Australian transect	770:846	Here we compare observed leaf water enrichment, along with previously published enrichment data from a similar north Australian transect, to Craig-Gordon-modelled predictions of leaf water isotopic enrichment.
27798741	4	18	theme	enrichment	909:918	arg1	predictions					874:884	Craig-Gordon-modelled predictions	852:884	Craig-Gordon-modelled predictions of leaf water isotopic enrichment	852:918	Here we compare observed leaf water enrichment, along with previously published enrichment data from a similar north Australian transect, to Craig-Gordon-modelled predictions of leaf water isotopic enrichment.
27798741	7	19	theme	pressure	1432:1439	arg1	predictor					1471:1479	a strong predictor	1462:1479	a strong predictor of the observed leaf cellulose δ18O values across one aridity gradient	1462:1550	The atmospheric-leaf vapour pressure relationship is also a strong predictor of the observed leaf cellulose δ18O values across one aridity gradient.
27798741	7	19	theme	pressure	1432:1439	arg1	relationship					1441:1452	The atmospheric-leaf vapour pressure relationship	1404:1452	The atmospheric-leaf vapour pressure relationship	1404:1452	The atmospheric-leaf vapour pressure relationship is also a strong predictor of the observed leaf cellulose δ18O values across one aridity gradient.
27798741	1	20	theme	previous	119:126	arg1	studies					128:134	Several previous studies	111:134	Several previous studies	111:134	Several previous studies have investigated the use of the stable hydrogen and oxygen isotope compositions in plant materials as indicators of palaeoclimate.
27798741	0	21	from	Australia	100:108	arg1	drivers					12:18	drivers	12:18	drivers of leaf water and cellulose stable isotope enrichment in Eucalyptus in northern Australia	12:108	Identifying drivers of leaf water and cellulose stable isotope enrichment in Eucalyptus in northern Australia.
27798741	7	22	theme	atmospheric-leaf	1408:1423	arg1	predictor					1471:1479	a strong predictor	1462:1479	a strong predictor of the observed leaf cellulose δ18O values across one aridity gradient	1462:1550	The atmospheric-leaf vapour pressure relationship is also a strong predictor of the observed leaf cellulose δ18O values across one aridity gradient.
27798741	7	22	theme	atmospheric-leaf	1408:1423	arg1	relationship					1441:1452	The atmospheric-leaf vapour pressure relationship	1404:1452	The atmospheric-leaf vapour pressure relationship	1404:1452	The atmospheric-leaf vapour pressure relationship is also a strong predictor of the observed leaf cellulose δ18O values across one aridity gradient.
27798741	6	23	dep	conditions	1257:1266	arg1	T > 37 °C					1279:1287	T > 37 °C	1279:1287	T > 37 °C	1279:1287	During exceptionally dry and hot conditions (RH < 21%, T > 37 °C) we observed strong deviations from Craig-Gordon predicted isotope enrichments caused by partial stomatal closure.
27798741	6	23	dep	conditions	1257:1266	arg1	%					1276:1276	RH < 21%	1269:1276	RH < 21%	1269:1276	During exceptionally dry and hot conditions (RH < 21%, T > 37 °C) we observed strong deviations from Craig-Gordon predicted isotope enrichments caused by partial stomatal closure.
27798741	6	24	theme	predicted	1338:1346	arg1	enrichments					1356:1366	Craig-Gordon predicted isotope enrichments	1325:1366	Craig-Gordon predicted isotope enrichments caused by partial stomatal closure	1325:1401	During exceptionally dry and hot conditions (RH < 21%, T > 37 °C) we observed strong deviations from Craig-Gordon predicted isotope enrichments caused by partial stomatal closure.
27798741	5	25	theme	leaf	1089:1092	arg1	pressure					1107:1114	atmospheric and internal leaf water vapour pressure	1064:1114	pressure	1107:1114	Our investigation of model parameters shows that observed 18O enrichment across the aridity gradients is dominated by the relationship between atmospheric and internal leaf water vapour pressure while 2H enrichment is driven mainly by variation in the water vapour-source water isotopic disequilibrium.
27798741	5	26	theme	parameters	948:957	arg1	investigation					925:937	Our investigation	921:937	Our investigation of model parameters	921:957	Our investigation of model parameters shows that observed 18O enrichment across the aridity gradients is dominated by the relationship between atmospheric and internal leaf water vapour pressure while 2H enrichment is driven mainly by variation in the water vapour-source water isotopic disequilibrium.
27798741	0	27	theme	isotope	55:61	arg1	enrichment					63:72	leaf water and cellulose stable isotope enrichment	23:72	enrichment	63:72	Identifying drivers of leaf water and cellulose stable isotope enrichment in Eucalyptus in northern Australia.
27798741	1	28	from	use	158:160	arg1	materials					226:234	plant materials	220:234	plant materials	220:234	Several previous studies have investigated the use of the stable hydrogen and oxygen isotope compositions in plant materials as indicators of palaeoclimate.
27798741	7	29	theme	vapour	1425:1430	arg1	predictor					1471:1479	a strong predictor	1462:1479	a strong predictor of the observed leaf cellulose δ18O values across one aridity gradient	1462:1550	The atmospheric-leaf vapour pressure relationship is also a strong predictor of the observed leaf cellulose δ18O values across one aridity gradient.
27798741	7	29	theme	vapour	1425:1430	arg1	relationship					1441:1452	The atmospheric-leaf vapour pressure relationship	1404:1452	The atmospheric-leaf vapour pressure relationship	1404:1452	The atmospheric-leaf vapour pressure relationship is also a strong predictor of the observed leaf cellulose δ18O values across one aridity gradient.
27798741	3	30	dep	measured	509:516	arg1	progress					488:495	progress	488:495	To progress this aim	485:504	To progress this aim we measured δ18O and δ2H values in eucalypt leaf and stem water and δ18O values in leaf cellulose, along with the isotopic compositions of water vapour, across a north-eastern Australian aridity gradient.
27798741	2	31	theme	organic	466:472	arg1	compounds					474:482	associated organic compounds	455:482	associated organic compounds	455:482	However, accurate interpretation relies on a detailed understanding of both physiological and environmental drivers of the variations in isotopic enrichments that occur in leaf water and associated organic compounds.
27798741	0	32	from	water	28:32	arg1	Eucalyptus					77:86	Eucalyptus	77:86	Eucalyptus	77:86	Identifying drivers of leaf water and cellulose stable isotope enrichment in Eucalyptus in northern Australia.
27798741	0	32	from	water	28:32	arg1	Australia					100:108	northern Australia	91:108	northern Australia	91:108	Identifying drivers of leaf water and cellulose stable isotope enrichment in Eucalyptus in northern Australia.
27798741	4	33	theme	leaf	736:739	arg1	enrichment					747:756	observed leaf water enrichment	727:756	observed leaf water enrichment	727:756	Here we compare observed leaf water enrichment, along with previously published enrichment data from a similar north Australian transect, to Craig-Gordon-modelled predictions of leaf water isotopic enrichment.
27798741	8	34	theme	δ18O	1614:1617	arg1	composition					1619:1629	leaf cellulose δ18O composition	1599:1629	leaf cellulose δ18O composition	1599:1629	Our finding supports a wider applicability of leaf cellulose δ18O composition as a climate proxy for atmospheric humidity conditions during the leaf growing season than previously documented.
27798741	2	35	theme	leaf	440:443	arg1	water					445:449	leaf water	440:449	leaf water	440:449	However, accurate interpretation relies on a detailed understanding of both physiological and environmental drivers of the variations in isotopic enrichments that occur in leaf water and associated organic compounds.
27798741	5	36	theme	18O	979:981	arg1	enrichment					983:992	observed 18O enrichment	970:992	observed 18O enrichment across the aridity gradients	970:1021	Our investigation of model parameters shows that observed 18O enrichment across the aridity gradients is dominated by the relationship between atmospheric and internal leaf water vapour pressure while 2H enrichment is driven mainly by variation in the water vapour-source water isotopic disequilibrium.
27798741	3	37	theme	water	645:649	arg1	vapour					651:656	water vapour	645:656	water vapour	645:656	To progress this aim we measured δ18O and δ2H values in eucalypt leaf and stem water and δ18O values in leaf cellulose, along with the isotopic compositions of water vapour, across a north-eastern Australian aridity gradient.
27798741	4	38	theme	similar	814:820	arg1	Australian					828:837	a similar north Australian	812:837	a similar north Australian transect	812:846	Here we compare observed leaf water enrichment, along with previously published enrichment data from a similar north Australian transect, to Craig-Gordon-modelled predictions of leaf water isotopic enrichment.
27798741	0	39	theme	water	28:32	arg1	drivers					12:18	drivers	12:18	drivers of leaf water and cellulose stable isotope enrichment in Eucalyptus in northern Australia	12:108	Identifying drivers of leaf water and cellulose stable isotope enrichment in Eucalyptus in northern Australia.
27798741	5	40	theme	water	1173:1177	arg1	disequilibrium					1208:1221	the water vapour-source water isotopic disequilibrium	1169:1221	the water vapour-source water isotopic disequilibrium	1169:1221	Our investigation of model parameters shows that observed 18O enrichment across the aridity gradients is dominated by the relationship between atmospheric and internal leaf water vapour pressure while 2H enrichment is driven mainly by variation in the water vapour-source water isotopic disequilibrium.
27798741	8	41	theme	climate	1636:1642	arg1	proxy					1644:1648	a climate proxy	1634:1648	a climate proxy for atmospheric humidity conditions	1634:1684	Our finding supports a wider applicability of leaf cellulose δ18O composition as a climate proxy for atmospheric humidity conditions during the leaf growing season than previously documented.
27798741	8	41	theme	climate	1636:1642	arg1	applicability					1582:1594	a wider applicability	1574:1594	a wider applicability of leaf cellulose δ18O composition	1574:1629	Our finding supports a wider applicability of leaf cellulose δ18O composition as a climate proxy for atmospheric humidity conditions during the leaf growing season than previously documented.
27798741	5	42	from	variation	1156:1164	arg1	disequilibrium					1208:1221	the water vapour-source water isotopic disequilibrium	1169:1221	the water vapour-source water isotopic disequilibrium	1169:1221	Our investigation of model parameters shows that observed 18O enrichment across the aridity gradients is dominated by the relationship between atmospheric and internal leaf water vapour pressure while 2H enrichment is driven mainly by variation in the water vapour-source water isotopic disequilibrium.
27798741	5	43	theme	water	1193:1197	arg1	disequilibrium					1208:1221	the water vapour-source water isotopic disequilibrium	1169:1221	the water vapour-source water isotopic disequilibrium	1169:1221	Our investigation of model parameters shows that observed 18O enrichment across the aridity gradients is dominated by the relationship between atmospheric and internal leaf water vapour pressure while 2H enrichment is driven mainly by variation in the water vapour-source water isotopic disequilibrium.
27798741	5	44	theme	atmospheric	1064:1074	arg1	pressure					1107:1114	atmospheric and internal leaf water vapour pressure	1064:1114	pressure	1107:1114	Our investigation of model parameters shows that observed 18O enrichment across the aridity gradients is dominated by the relationship between atmospheric and internal leaf water vapour pressure while 2H enrichment is driven mainly by variation in the water vapour-source water isotopic disequilibrium.
27798741	2	45	from	enrichments	414:424	arg1	drivers					376:382	both physiological and environmental drivers	339:382	both physiological and environmental drivers of the variations in isotopic enrichments that occur in leaf water and associated organic compounds	339:482	However, accurate interpretation relies on a detailed understanding of both physiological and environmental drivers of the variations in isotopic enrichments that occur in leaf water and associated organic compounds.
27798741	4	46	theme	enrichment	791:800	arg1	data					802:805	previously published enrichment data	770:805	previously published enrichment data from a similar north Australian transect	770:846	Here we compare observed leaf water enrichment, along with previously published enrichment data from a similar north Australian transect, to Craig-Gordon-modelled predictions of leaf water isotopic enrichment.
27798741	1	47	theme	plant	220:224	arg1	materials					226:234	plant materials	220:234	plant materials	220:234	Several previous studies have investigated the use of the stable hydrogen and oxygen isotope compositions in plant materials as indicators of palaeoclimate.
27798741	8	48	theme	humidity	1666:1673	arg1	conditions					1675:1684	atmospheric humidity conditions	1654:1684	atmospheric humidity conditions	1654:1684	Our finding supports a wider applicability of leaf cellulose δ18O composition as a climate proxy for atmospheric humidity conditions during the leaf growing season than previously documented.
27798741	3	49	theme	δ2H	527:529	arg1	values					531:536	δ18O and δ2H values	518:536	δ18O and δ2H values in eucalypt leaf and stem water	518:568	To progress this aim we measured δ18O and δ2H values in eucalypt leaf and stem water and δ18O values in leaf cellulose, along with the isotopic compositions of water vapour, across a north-eastern Australian aridity gradient.
27798741	5	50	dep	pressure	1107:1114	arg1	driven					1139:1144	driven	1139:1144	is driven mainly by variation in the water vapour-source water isotopic disequilibrium	1136:1221	Our investigation of model parameters shows that observed 18O enrichment across the aridity gradients is dominated by the relationship between atmospheric and internal leaf water vapour pressure while 2H enrichment is driven mainly by variation in the water vapour-source water isotopic disequilibrium.
27798741	6	51	theme	Craig-Gordon	1325:1336	arg1	enrichments					1356:1366	Craig-Gordon predicted isotope enrichments	1325:1366	Craig-Gordon predicted isotope enrichments caused by partial stomatal closure	1325:1401	During exceptionally dry and hot conditions (RH < 21%, T > 37 °C) we observed strong deviations from Craig-Gordon predicted isotope enrichments caused by partial stomatal closure.
27798741	2	52	theme	drivers	376:382	arg1	understanding					322:334	a detailed understanding	311:334	a detailed understanding of both physiological and environmental drivers of the variations in isotopic enrichments that occur in leaf water and associated organic compounds	311:482	However, accurate interpretation relies on a detailed understanding of both physiological and environmental drivers of the variations in isotopic enrichments that occur in leaf water and associated organic compounds.
27798741	7	53	theme	aridity	1535:1541	arg1	gradient					1543:1550	one aridity gradient	1531:1550	one aridity gradient	1531:1550	The atmospheric-leaf vapour pressure relationship is also a strong predictor of the observed leaf cellulose δ18O values across one aridity gradient.
27798741	8	54	theme	leaf	1697:1700	arg1	season					1710:1715	the leaf growing season than previously documented	1693:1742	the leaf growing season than previously documented	1693:1742	Our finding supports a wider applicability of leaf cellulose δ18O composition as a climate proxy for atmospheric humidity conditions during the leaf growing season than previously documented.
27798741	3	55	theme	Australian	682:691	arg1	gradient					701:708	a north-eastern Australian aridity gradient	666:708	a north-eastern Australian aridity gradient	666:708	To progress this aim we measured δ18O and δ2H values in eucalypt leaf and stem water and δ18O values in leaf cellulose, along with the isotopic compositions of water vapour, across a north-eastern Australian aridity gradient.
27798741	4	56	theme	leaf	889:892	arg1	enrichment					909:918	leaf water isotopic enrichment	889:918	leaf water isotopic enrichment	889:918	Here we compare observed leaf water enrichment, along with previously published enrichment data from a similar north Australian transect, to Craig-Gordon-modelled predictions of leaf water isotopic enrichment.
27798741	7	57	theme	cellulose	1502:1510	arg1	values					1517:1522	the observed leaf cellulose δ18O values	1484:1522	the observed leaf cellulose δ18O values	1484:1522	The atmospheric-leaf vapour pressure relationship is also a strong predictor of the observed leaf cellulose δ18O values across one aridity gradient.
27798741	3	58	from	values	531:536	arg1	leaf					550:553	eucalypt leaf	541:553	eucalypt leaf	541:553	To progress this aim we measured δ18O and δ2H values in eucalypt leaf and stem water and δ18O values in leaf cellulose, along with the isotopic compositions of water vapour, across a north-eastern Australian aridity gradient.
27798741	3	58	from	values	531:536	arg1	water					564:568	stem water	559:568	stem water	559:568	To progress this aim we measured δ18O and δ2H values in eucalypt leaf and stem water and δ18O values in leaf cellulose, along with the isotopic compositions of water vapour, across a north-eastern Australian aridity gradient.
27798741	3	58	from	values	531:536	arg1	cellulose					594:602	leaf cellulose	589:602	leaf cellulose	589:602	To progress this aim we measured δ18O and δ2H values in eucalypt leaf and stem water and δ18O values in leaf cellulose, along with the isotopic compositions of water vapour, across a north-eastern Australian aridity gradient.
27798741	2	59	theme	accurate	277:284	arg1	interpretation					286:299	accurate interpretation	277:299	accurate interpretation	277:299	However, accurate interpretation relies on a detailed understanding of both physiological and environmental drivers of the variations in isotopic enrichments that occur in leaf water and associated organic compounds.
27798741	0	60	theme	cellulose	38:46	arg1	enrichment					63:72	leaf water and cellulose stable isotope enrichment	23:72	enrichment	63:72	Identifying drivers of leaf water and cellulose stable isotope enrichment in Eucalyptus in northern Australia.
27798741	7	61	theme	δ18O	1512:1515	arg1	values					1517:1522	the observed leaf cellulose δ18O values	1484:1522	the observed leaf cellulose δ18O values	1484:1522	The atmospheric-leaf vapour pressure relationship is also a strong predictor of the observed leaf cellulose δ18O values across one aridity gradient.
27798741	2	62	from	drivers	376:382	arg1	enrichments					414:424	isotopic enrichments	405:424	isotopic enrichments that occur in leaf water and associated organic compounds	405:482	However, accurate interpretation relies on a detailed understanding of both physiological and environmental drivers of the variations in isotopic enrichments that occur in leaf water and associated organic compounds.
27798741	7	63	theme	observed	1488:1495	arg1	values					1517:1522	the observed leaf cellulose δ18O values	1484:1522	the observed leaf cellulose δ18O values	1484:1522	The atmospheric-leaf vapour pressure relationship is also a strong predictor of the observed leaf cellulose δ18O values across one aridity gradient.
27798741	1	64	theme	hydrogen	176:183	arg1	indicators					239:248	indicators	239:248	indicators of palaeoclimate	239:265	Several previous studies have investigated the use of the stable hydrogen and oxygen isotope compositions in plant materials as indicators of palaeoclimate.
27798741	1	64	theme	hydrogen	176:183	arg1	use					158:160	the use	154:160	the use of the stable hydrogen and oxygen isotope compositions in plant materials	154:234	Several previous studies have investigated the use of the stable hydrogen and oxygen isotope compositions in plant materials as indicators of palaeoclimate.
27798741	8	65	theme	wider	1576:1580	arg1	proxy					1644:1648	a climate proxy	1634:1648	a climate proxy for atmospheric humidity conditions	1634:1684	Our finding supports a wider applicability of leaf cellulose δ18O composition as a climate proxy for atmospheric humidity conditions during the leaf growing season than previously documented.
27798741	8	65	theme	wider	1576:1580	arg1	applicability					1582:1594	a wider applicability	1574:1594	a wider applicability of leaf cellulose δ18O composition	1574:1629	Our finding supports a wider applicability of leaf cellulose δ18O composition as a climate proxy for atmospheric humidity conditions during the leaf growing season than previously documented.
27798741	3	66	theme	stem	559:562	arg1	water					564:568	stem water	559:568	stem water	559:568	To progress this aim we measured δ18O and δ2H values in eucalypt leaf and stem water and δ18O values in leaf cellulose, along with the isotopic compositions of water vapour, across a north-eastern Australian aridity gradient.
27798741	4	67	dep	Australian	828:837	arg1	transect					839:846	transect	839:846	transect	839:846	Here we compare observed leaf water enrichment, along with previously published enrichment data from a similar north Australian transect, to Craig-Gordon-modelled predictions of leaf water isotopic enrichment.
27798741	3	68	theme	δ18O	574:577	arg1	values					579:584	δ18O values	574:584	δ18O values in leaf cellulose	574:602	To progress this aim we measured δ18O and δ2H values in eucalypt leaf and stem water and δ18O values in leaf cellulose, along with the isotopic compositions of water vapour, across a north-eastern Australian aridity gradient.
27798741	0	69	from	Eucalyptus	77:86	arg1	drivers					12:18	drivers	12:18	drivers of leaf water and cellulose stable isotope enrichment in Eucalyptus in northern Australia	12:108	Identifying drivers of leaf water and cellulose stable isotope enrichment in Eucalyptus in northern Australia.
27798741	7	70	theme	strong	1464:1469	arg1	predictor					1471:1479	a strong predictor	1462:1479	a strong predictor of the observed leaf cellulose δ18O values across one aridity gradient	1462:1550	The atmospheric-leaf vapour pressure relationship is also a strong predictor of the observed leaf cellulose δ18O values across one aridity gradient.
27798741	7	70	theme	strong	1464:1469	arg1	relationship					1441:1452	The atmospheric-leaf vapour pressure relationship	1404:1452	The atmospheric-leaf vapour pressure relationship	1404:1452	The atmospheric-leaf vapour pressure relationship is also a strong predictor of the observed leaf cellulose δ18O values across one aridity gradient.
27798741	6	71	theme	partial	1378:1384	arg1	closure					1395:1401	partial stomatal closure	1378:1401	partial stomatal closure	1378:1401	During exceptionally dry and hot conditions (RH < 21%, T > 37 °C) we observed strong deviations from Craig-Gordon predicted isotope enrichments caused by partial stomatal closure.
27798741	3	72	theme	leaf	589:592	arg1	cellulose					594:602	leaf cellulose	589:602	leaf cellulose	589:602	To progress this aim we measured δ18O and δ2H values in eucalypt leaf and stem water and δ18O values in leaf cellulose, along with the isotopic compositions of water vapour, across a north-eastern Australian aridity gradient.
27798741	1	73	theme	compositions	204:215	arg1	indicators					239:248	indicators	239:248	indicators of palaeoclimate	239:265	Several previous studies have investigated the use of the stable hydrogen and oxygen isotope compositions in plant materials as indicators of palaeoclimate.
27798741	1	73	theme	compositions	204:215	arg1	use					158:160	the use	154:160	the use of the stable hydrogen and oxygen isotope compositions in plant materials	154:234	Several previous studies have investigated the use of the stable hydrogen and oxygen isotope compositions in plant materials as indicators of palaeoclimate.
27798741	0	74	from	enrichment	63:72	arg1	Eucalyptus					77:86	Eucalyptus	77:86	Eucalyptus	77:86	Identifying drivers of leaf water and cellulose stable isotope enrichment in Eucalyptus in northern Australia.
27798741	0	74	from	enrichment	63:72	arg1	Australia					100:108	northern Australia	91:108	northern Australia	91:108	Identifying drivers of leaf water and cellulose stable isotope enrichment in Eucalyptus in northern Australia.
27798741	6	75	from	enrichments	1356:1366	arg1	deviations					1309:1318	strong deviations	1302:1318	strong deviations from Craig-Gordon predicted isotope enrichments caused by partial stomatal closure	1302:1401	During exceptionally dry and hot conditions (RH < 21%, T > 37 °C) we observed strong deviations from Craig-Gordon predicted isotope enrichments caused by partial stomatal closure.
27798741	5	76	theme	2H	1122:1123	arg1	enrichment					1125:1134	2H enrichment	1122:1134	2H enrichment	1122:1134	Our investigation of model parameters shows that observed 18O enrichment across the aridity gradients is dominated by the relationship between atmospheric and internal leaf water vapour pressure while 2H enrichment is driven mainly by variation in the water vapour-source water isotopic disequilibrium.
27798741	1	77	theme	Several	111:117	arg1	studies					128:134	Several previous studies	111:134	Several previous studies	111:134	Several previous studies have investigated the use of the stable hydrogen and oxygen isotope compositions in plant materials as indicators of palaeoclimate.
27798741	4	78	theme	isotopic	900:907	arg1	enrichment					909:918	leaf water isotopic enrichment	889:918	leaf water isotopic enrichment	889:918	Here we compare observed leaf water enrichment, along with previously published enrichment data from a similar north Australian transect, to Craig-Gordon-modelled predictions of leaf water isotopic enrichment.
27798741	6	79	theme	isotope	1348:1354	arg1	enrichments					1356:1366	Craig-Gordon predicted isotope enrichments	1325:1366	Craig-Gordon predicted isotope enrichments caused by partial stomatal closure	1325:1401	During exceptionally dry and hot conditions (RH < 21%, T > 37 °C) we observed strong deviations from Craig-Gordon predicted isotope enrichments caused by partial stomatal closure.
27798741	5	80	theme	internal	1080:1087	arg1	pressure					1107:1114	atmospheric and internal leaf water vapour pressure	1064:1114	pressure	1107:1114	Our investigation of model parameters shows that observed 18O enrichment across the aridity gradients is dominated by the relationship between atmospheric and internal leaf water vapour pressure while 2H enrichment is driven mainly by variation in the water vapour-source water isotopic disequilibrium.
27798741	5	81	theme	model	942:946	arg1	parameters					948:957	model parameters	942:957	model parameters	942:957	Our investigation of model parameters shows that observed 18O enrichment across the aridity gradients is dominated by the relationship between atmospheric and internal leaf water vapour pressure while 2H enrichment is driven mainly by variation in the water vapour-source water isotopic disequilibrium.
27798741	6	82	theme	hot	1253:1255	arg1	conditions					1257:1266	exceptionally dry and hot conditions	1231:1266	exceptionally dry and hot conditions (RH < 21%, T > 37 °C)	1231:1288	During exceptionally dry and hot conditions (RH < 21%, T > 37 °C) we observed strong deviations from Craig-Gordon predicted isotope enrichments caused by partial stomatal closure.
27798741	0	83	theme	enrichment	63:72	arg1	drivers					12:18	drivers	12:18	drivers of leaf water and cellulose stable isotope enrichment in Eucalyptus in northern Australia	12:108	Identifying drivers of leaf water and cellulose stable isotope enrichment in Eucalyptus in northern Australia.
27798741	5	84	theme	water	1094:1098	arg1	pressure					1107:1114	atmospheric and internal leaf water vapour pressure	1064:1114	pressure	1107:1114	Our investigation of model parameters shows that observed 18O enrichment across the aridity gradients is dominated by the relationship between atmospheric and internal leaf water vapour pressure while 2H enrichment is driven mainly by variation in the water vapour-source water isotopic disequilibrium.
27798741	1	85	theme	oxygen	189:194	arg1	compositions					204:215	the stable hydrogen and oxygen isotope compositions	165:215	compositions	204:215	Several previous studies have investigated the use of the stable hydrogen and oxygen isotope compositions in plant materials as indicators of palaeoclimate.
27798741	6	86	theme	dry	1245:1247	arg1	conditions					1257:1266	exceptionally dry and hot conditions	1231:1266	exceptionally dry and hot conditions (RH < 21%, T > 37 °C)	1231:1288	During exceptionally dry and hot conditions (RH < 21%, T > 37 °C) we observed strong deviations from Craig-Gordon predicted isotope enrichments caused by partial stomatal closure.
27798741	0	87	from	drivers	12:18	arg1	Eucalyptus					77:86	Eucalyptus	77:86	Eucalyptus	77:86	Identifying drivers of leaf water and cellulose stable isotope enrichment in Eucalyptus in northern Australia.
27798741	0	87	from	drivers	12:18	arg1	Australia					100:108	northern Australia	91:108	northern Australia	91:108	Identifying drivers of leaf water and cellulose stable isotope enrichment in Eucalyptus in northern Australia.
27798741	0	88	theme	northern	91:98	arg1	Australia					100:108	northern Australia	91:108	northern Australia	91:108	Identifying drivers of leaf water and cellulose stable isotope enrichment in Eucalyptus in northern Australia.
27798741	8	89	theme	cellulose	1604:1612	arg1	composition					1619:1629	leaf cellulose δ18O composition	1599:1629	leaf cellulose δ18O composition	1599:1629	Our finding supports a wider applicability of leaf cellulose δ18O composition as a climate proxy for atmospheric humidity conditions during the leaf growing season than previously documented.
27798741	2	90	theme	isotopic	405:412	arg1	enrichments					414:424	isotopic enrichments	405:424	isotopic enrichments that occur in leaf water and associated organic compounds	405:482	However, accurate interpretation relies on a detailed understanding of both physiological and environmental drivers of the variations in isotopic enrichments that occur in leaf water and associated organic compounds.
27798741	2	91	theme	associated	455:464	arg1	compounds					474:482	associated organic compounds	455:482	associated organic compounds	455:482	However, accurate interpretation relies on a detailed understanding of both physiological and environmental drivers of the variations in isotopic enrichments that occur in leaf water and associated organic compounds.
27798741	8	92	theme	composition	1619:1629	arg1	proxy					1644:1648	a climate proxy	1634:1648	a climate proxy for atmospheric humidity conditions	1634:1684	Our finding supports a wider applicability of leaf cellulose δ18O composition as a climate proxy for atmospheric humidity conditions during the leaf growing season than previously documented.
27798741	8	92	theme	composition	1619:1629	arg1	applicability					1582:1594	a wider applicability	1574:1594	a wider applicability of leaf cellulose δ18O composition	1574:1629	Our finding supports a wider applicability of leaf cellulose δ18O composition as a climate proxy for atmospheric humidity conditions during the leaf growing season than previously documented.
27798741	4	93	theme	observed	727:734	arg1	enrichment					747:756	observed leaf water enrichment	727:756	observed leaf water enrichment	727:756	Here we compare observed leaf water enrichment, along with previously published enrichment data from a similar north Australian transect, to Craig-Gordon-modelled predictions of leaf water isotopic enrichment.
27798741	3	94	from	values	579:584	arg1	leaf					550:553	eucalypt leaf	541:553	eucalypt leaf	541:553	To progress this aim we measured δ18O and δ2H values in eucalypt leaf and stem water and δ18O values in leaf cellulose, along with the isotopic compositions of water vapour, across a north-eastern Australian aridity gradient.
27798741	3	94	from	values	579:584	arg1	water					564:568	stem water	559:568	stem water	559:568	To progress this aim we measured δ18O and δ2H values in eucalypt leaf and stem water and δ18O values in leaf cellulose, along with the isotopic compositions of water vapour, across a north-eastern Australian aridity gradient.
27798741	3	94	from	values	579:584	arg1	cellulose					594:602	leaf cellulose	589:602	leaf cellulose	589:602	To progress this aim we measured δ18O and δ2H values in eucalypt leaf and stem water and δ18O values in leaf cellulose, along with the isotopic compositions of water vapour, across a north-eastern Australian aridity gradient.
27798741	2	95	theme	variations	391:400	arg1	drivers					376:382	both physiological and environmental drivers	339:382	both physiological and environmental drivers of the variations in isotopic enrichments that occur in leaf water and associated organic compounds	339:482	However, accurate interpretation relies on a detailed understanding of both physiological and environmental drivers of the variations in isotopic enrichments that occur in leaf water and associated organic compounds.
27798741	0	96	theme	leaf	23:26	arg1	water					28:32	leaf water and cellulose stable isotope enrichment	23:72	water	28:32	Identifying drivers of leaf water and cellulose stable isotope enrichment in Eucalyptus in northern Australia.
27798741	5	97	theme	observed	970:977	arg1	enrichment					983:992	observed 18O enrichment	970:992	observed 18O enrichment across the aridity gradients	970:1021	Our investigation of model parameters shows that observed 18O enrichment across the aridity gradients is dominated by the relationship between atmospheric and internal leaf water vapour pressure while 2H enrichment is driven mainly by variation in the water vapour-source water isotopic disequilibrium.
27798741	4	98	theme	water	741:745	arg1	enrichment					747:756	observed leaf water enrichment	727:756	observed leaf water enrichment	727:756	Here we compare observed leaf water enrichment, along with previously published enrichment data from a similar north Australian transect, to Craig-Gordon-modelled predictions of leaf water isotopic enrichment.
27798741	3	99	theme	isotopic	620:627	arg1	compositions					629:640	the isotopic compositions	616:640	the isotopic compositions of water vapour	616:656	To progress this aim we measured δ18O and δ2H values in eucalypt leaf and stem water and δ18O values in leaf cellulose, along with the isotopic compositions of water vapour, across a north-eastern Australian aridity gradient.
27798741	0	100	theme	stable	48:53	arg1	enrichment					63:72	leaf water and cellulose stable isotope enrichment	23:72	enrichment	63:72	Identifying drivers of leaf water and cellulose stable isotope enrichment in Eucalyptus in northern Australia.
27798741	5	101	theme	vapour-source	1179:1191	arg1	disequilibrium					1208:1221	the water vapour-source water isotopic disequilibrium	1169:1221	the water vapour-source water isotopic disequilibrium	1169:1221	Our investigation of model parameters shows that observed 18O enrichment across the aridity gradients is dominated by the relationship between atmospheric and internal leaf water vapour pressure while 2H enrichment is driven mainly by variation in the water vapour-source water isotopic disequilibrium.
27798741	2	102	from	variations	391:400	arg1	enrichments					414:424	isotopic enrichments	405:424	isotopic enrichments that occur in leaf water and associated organic compounds	405:482	However, accurate interpretation relies on a detailed understanding of both physiological and environmental drivers of the variations in isotopic enrichments that occur in leaf water and associated organic compounds.
27798741	8	103	theme	atmospheric	1654:1664	arg1	conditions					1675:1684	atmospheric humidity conditions	1654:1684	atmospheric humidity conditions	1654:1684	Our finding supports a wider applicability of leaf cellulose δ18O composition as a climate proxy for atmospheric humidity conditions during the leaf growing season than previously documented.
27798741	4	104	theme	north	822:826	arg1	Australian					828:837	a similar north Australian	812:837	a similar north Australian transect	812:846	Here we compare observed leaf water enrichment, along with previously published enrichment data from a similar north Australian transect, to Craig-Gordon-modelled predictions of leaf water isotopic enrichment.
27798741	2	105	theme	physiological	344:356	arg1	drivers					376:382	both physiological and environmental drivers	339:382	both physiological and environmental drivers of the variations in isotopic enrichments that occur in leaf water and associated organic compounds	339:482	However, accurate interpretation relies on a detailed understanding of both physiological and environmental drivers of the variations in isotopic enrichments that occur in leaf water and associated organic compounds.
27798741	5	106	theme	isotopic	1199:1206	arg1	disequilibrium					1208:1221	the water vapour-source water isotopic disequilibrium	1169:1221	the water vapour-source water isotopic disequilibrium	1169:1221	Our investigation of model parameters shows that observed 18O enrichment across the aridity gradients is dominated by the relationship between atmospheric and internal leaf water vapour pressure while 2H enrichment is driven mainly by variation in the water vapour-source water isotopic disequilibrium.
27798741	5	107	theme	aridity	1005:1011	arg1	gradients					1013:1021	the aridity gradients	1001:1021	the aridity gradients	1001:1021	Our investigation of model parameters shows that observed 18O enrichment across the aridity gradients is dominated by the relationship between atmospheric and internal leaf water vapour pressure while 2H enrichment is driven mainly by variation in the water vapour-source water isotopic disequilibrium.
27798741	3	108	theme	vapour	651:656	arg1	compositions					629:640	the isotopic compositions	616:640	the isotopic compositions of water vapour	616:656	To progress this aim we measured δ18O and δ2H values in eucalypt leaf and stem water and δ18O values in leaf cellulose, along with the isotopic compositions of water vapour, across a north-eastern Australian aridity gradient.
27798741	4	109	theme	published	781:789	arg1	data					802:805	previously published enrichment data	770:805	previously published enrichment data from a similar north Australian transect	770:846	Here we compare observed leaf water enrichment, along with previously published enrichment data from a similar north Australian transect, to Craig-Gordon-modelled predictions of leaf water isotopic enrichment.
27798741	3	110	theme	aridity	693:699	arg1	gradient					701:708	a north-eastern Australian aridity gradient	666:708	a north-eastern Australian aridity gradient	666:708	To progress this aim we measured δ18O and δ2H values in eucalypt leaf and stem water and δ18O values in leaf cellulose, along with the isotopic compositions of water vapour, across a north-eastern Australian aridity gradient.
29032210	0	0	theme	RAW264.7	117:124	arg1	macrophage					126:135	lipopolysaccharide-stimulated RAW264.7 macrophage	87:135	lipopolysaccharide-stimulated RAW264.7 macrophage	87:135	Chemical composition of Cyclocarya paliurus polysaccharide and inflammatory effects in lipopolysaccharide-stimulated RAW264.7 macrophage.
29032210	8	1	theme	PGE2	1629:1632	arg1	release					1608:1614	the release	1604:1614	the release of IL-1β and PGE2	1604:1632	CPP-3 and LPS accounted for synergistic effect on the release of NO and TNF-α, CPP-3 and LPS accounted for antagonistic effect on the release of IL-1β and PGE2.
29032210	1	2	theme	RAW264.7	298:305	arg1	macrophage					307:316	lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage	262:316	lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage	262:316	This study was designed to study the chemical composition of Cyclocarya paliurus polysaccharide and inflammatory effects in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage.
29032210	0	3	theme	lipopolysaccharide-stimulated	87:115	arg1	macrophage					126:135	lipopolysaccharide-stimulated RAW264.7 macrophage	87:135	lipopolysaccharide-stimulated RAW264.7 macrophage	87:135	Chemical composition of Cyclocarya paliurus polysaccharide and inflammatory effects in lipopolysaccharide-stimulated RAW264.7 macrophage.
29032210	0	4	from	composition	9:19	arg1	macrophage					126:135	lipopolysaccharide-stimulated RAW264.7 macrophage	87:135	lipopolysaccharide-stimulated RAW264.7 macrophage	87:135	Chemical composition of Cyclocarya paliurus polysaccharide and inflammatory effects in lipopolysaccharide-stimulated RAW264.7 macrophage.
29032210	5	5	theme	monosaccharides	1025:1039	arg1	Rha					1065:1067	Rha	1065:1067	Rha	1065:1067	CPP-3 contains six monosaccharides, of which are rhamnose (Rha), arabinose (Ara), xylose (Xyl), mannose (Man), glucose (Glu), galactose (Gal), the molar ratio of six monosaccharides is 0.060:0.109:0.053:0.128:0.293:0.357.
29032210	5	5	theme	monosaccharides	1025:1039	arg1	mannose					1102:1108	mannose	1102:1108	mannose (Man)	1102:1114	CPP-3 contains six monosaccharides, of which are rhamnose (Rha), arabinose (Ara), xylose (Xyl), mannose (Man), glucose (Glu), galactose (Gal), the molar ratio of six monosaccharides is 0.060:0.109:0.053:0.128:0.293:0.357.
29032210	5	5	theme	monosaccharides	1025:1039	arg1	xylose					1088:1093	xylose	1088:1093	xylose (Xyl)	1088:1099	CPP-3 contains six monosaccharides, of which are rhamnose (Rha), arabinose (Ara), xylose (Xyl), mannose (Man), glucose (Glu), galactose (Gal), the molar ratio of six monosaccharides is 0.060:0.109:0.053:0.128:0.293:0.357.
29032210	5	5	theme	monosaccharides	1025:1039	arg1	arabinose					1071:1079	arabinose	1071:1079	arabinose (Ara)	1071:1085	CPP-3 contains six monosaccharides, of which are rhamnose (Rha), arabinose (Ara), xylose (Xyl), mannose (Man), glucose (Glu), galactose (Gal), the molar ratio of six monosaccharides is 0.060:0.109:0.053:0.128:0.293:0.357.
29032210	5	5	theme	monosaccharides	1025:1039	arg1	rhamnose					1055:1062	rhamnose	1055:1062	rhamnose (Rha)	1055:1068	CPP-3 contains six monosaccharides, of which are rhamnose (Rha), arabinose (Ara), xylose (Xyl), mannose (Man), glucose (Glu), galactose (Gal), the molar ratio of six monosaccharides is 0.060:0.109:0.053:0.128:0.293:0.357.
29032210	1	6	from	composition	184:194	arg1	macrophage					307:316	lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage	262:316	lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage	262:316	This study was designed to study the chemical composition of Cyclocarya paliurus polysaccharide and inflammatory effects in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage.
29032210	2	7	theme	polysaccharide	385:398	arg1	elution					325:331	A new elution	319:331	A new elution (0.3% NaCl aqueous solution) of Cyclocarya paliurus polysaccharide (CPP-3)	319:406	A new elution (0.3% NaCl aqueous solution) of Cyclocarya paliurus polysaccharide (CPP-3) was characterized by different methods such as fourier transform infrared spectra (FT-IR), UV-vis, gas chromatography-mass spectrometry (GC-MS), high performance gel chromatography (HPGLC) and scanning electron microscopy (SEM).
29032210	2	7	theme	polysaccharide	385:398	arg1	solution					352:359	0.3% NaCl aqueous solution	334:359	0.3% NaCl aqueous solution	334:359	A new elution (0.3% NaCl aqueous solution) of Cyclocarya paliurus polysaccharide (CPP-3) was characterized by different methods such as fourier transform infrared spectra (FT-IR), UV-vis, gas chromatography-mass spectrometry (GC-MS), high performance gel chromatography (HPGLC) and scanning electron microscopy (SEM).
29032210	6	8	theme	IL-1β	1350:1354	arg1	levels					1333:1338	the levels	1329:1338	the levels of TNF-α, IL-1β and PGE2 (P<0.01)	1329:1372	CPP-3 increased the amount of NO released from mouse macrophage RAW264.7 and significantly increased the levels of TNF-α, IL-1β and PGE2 (P<0.01).
29032210	6	9	theme	NO	1258:1259	arg1	NO					1258:1259	NO	1258:1259	NO	1258:1259	CPP-3 increased the amount of NO released from mouse macrophage RAW264.7 and significantly increased the levels of TNF-α, IL-1β and PGE2 (P<0.01).
29032210	6	9	theme	NO	1258:1259	arg1	amount					1248:1253	the amount	1244:1253	the amount of NO released from mouse macrophage RAW264.7	1244:1299	CPP-3 increased the amount of NO released from mouse macrophage RAW264.7 and significantly increased the levels of TNF-α, IL-1β and PGE2 (P<0.01).
29032210	2	10	theme	electron	610:617	arg1	microscopy					619:628	scanning electron microscopy	601:628	scanning electron microscopy (SEM)	601:634	A new elution (0.3% NaCl aqueous solution) of Cyclocarya paliurus polysaccharide (CPP-3) was characterized by different methods such as fourier transform infrared spectra (FT-IR), UV-vis, gas chromatography-mass spectrometry (GC-MS), high performance gel chromatography (HPGLC) and scanning electron microscopy (SEM).
29032210	2	10	theme	electron	610:617	arg1	SEM					631:633	SEM	631:633	SEM	631:633	A new elution (0.3% NaCl aqueous solution) of Cyclocarya paliurus polysaccharide (CPP-3) was characterized by different methods such as fourier transform infrared spectra (FT-IR), UV-vis, gas chromatography-mass spectrometry (GC-MS), high performance gel chromatography (HPGLC) and scanning electron microscopy (SEM).
29032210	3	11	theme	enzyme	847:852	arg1	system					866:871	enzyme immunoassay system	847:871	enzyme immunoassay system	847:871	Cell viability was measured by MTT test, phagocytosis assay was measured by Neutral red uptake assay, nitrite was measured by Griess assay, TNF-α and IL-1β analysis were measured by ELISA, PGE2 was measured by enzyme immunoassay system.
29032210	8	12	theme	NO	1539:1540	arg1	release					1528:1534	the release	1524:1534	the release of NO	1524:1540	CPP-3 and LPS accounted for synergistic effect on the release of NO and TNF-α, CPP-3 and LPS accounted for antagonistic effect on the release of IL-1β and PGE2.
29032210	3	13	theme	uptake	725:730	arg1	assay					732:736	Neutral red uptake assay	713:736	Neutral red uptake assay	713:736	Cell viability was measured by MTT test, phagocytosis assay was measured by Neutral red uptake assay, nitrite was measured by Griess assay, TNF-α and IL-1β analysis were measured by ELISA, PGE2 was measured by enzyme immunoassay system.
29032210	2	14	theme	scanning	601:608	arg1	microscopy					619:628	scanning electron microscopy	601:628	scanning electron microscopy (SEM)	601:634	A new elution (0.3% NaCl aqueous solution) of Cyclocarya paliurus polysaccharide (CPP-3) was characterized by different methods such as fourier transform infrared spectra (FT-IR), UV-vis, gas chromatography-mass spectrometry (GC-MS), high performance gel chromatography (HPGLC) and scanning electron microscopy (SEM).
29032210	2	14	theme	scanning	601:608	arg1	SEM					631:633	SEM	631:633	SEM	631:633	A new elution (0.3% NaCl aqueous solution) of Cyclocarya paliurus polysaccharide (CPP-3) was characterized by different methods such as fourier transform infrared spectra (FT-IR), UV-vis, gas chromatography-mass spectrometry (GC-MS), high performance gel chromatography (HPGLC) and scanning electron microscopy (SEM).
29032210	1	15	theme	Cyclocarya	199:208	arg1	polysaccharide					219:232	Cyclocarya paliurus polysaccharide	199:232	Cyclocarya paliurus polysaccharide	199:232	This study was designed to study the chemical composition of Cyclocarya paliurus polysaccharide and inflammatory effects in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage.
29032210	3	16	theme	Neutral	713:719	arg1	assay					732:736	Neutral red uptake assay	713:736	Neutral red uptake assay	713:736	Cell viability was measured by MTT test, phagocytosis assay was measured by Neutral red uptake assay, nitrite was measured by Griess assay, TNF-α and IL-1β analysis were measured by ELISA, PGE2 was measured by enzyme immunoassay system.
29032210	2	17	theme	UV-vis	499:504	arg1	GC-MS					545:549	GC-MS	545:549	GC-MS	545:549	A new elution (0.3% NaCl aqueous solution) of Cyclocarya paliurus polysaccharide (CPP-3) was characterized by different methods such as fourier transform infrared spectra (FT-IR), UV-vis, gas chromatography-mass spectrometry (GC-MS), high performance gel chromatography (HPGLC) and scanning electron microscopy (SEM).
29032210	2	17	theme	UV-vis	499:504	arg1	spectrometry					531:542	UV-vis, gas chromatography-mass spectrometry	499:542	spectrometry	531:542	A new elution (0.3% NaCl aqueous solution) of Cyclocarya paliurus polysaccharide (CPP-3) was characterized by different methods such as fourier transform infrared spectra (FT-IR), UV-vis, gas chromatography-mass spectrometry (GC-MS), high performance gel chromatography (HPGLC) and scanning electron microscopy (SEM).
29032210	2	18	theme	new	321:323	arg1	elution					325:331	A new elution	319:331	A new elution (0.3% NaCl aqueous solution) of Cyclocarya paliurus polysaccharide (CPP-3)	319:406	A new elution (0.3% NaCl aqueous solution) of Cyclocarya paliurus polysaccharide (CPP-3) was characterized by different methods such as fourier transform infrared spectra (FT-IR), UV-vis, gas chromatography-mass spectrometry (GC-MS), high performance gel chromatography (HPGLC) and scanning electron microscopy (SEM).
29032210	2	18	theme	new	321:323	arg1	solution					352:359	0.3% NaCl aqueous solution	334:359	0.3% NaCl aqueous solution	334:359	A new elution (0.3% NaCl aqueous solution) of Cyclocarya paliurus polysaccharide (CPP-3) was characterized by different methods such as fourier transform infrared spectra (FT-IR), UV-vis, gas chromatography-mass spectrometry (GC-MS), high performance gel chromatography (HPGLC) and scanning electron microscopy (SEM).
29032210	1	19	theme	paliurus	210:217	arg1	polysaccharide					219:232	Cyclocarya paliurus polysaccharide	199:232	Cyclocarya paliurus polysaccharide	199:232	This study was designed to study the chemical composition of Cyclocarya paliurus polysaccharide and inflammatory effects in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage.
29032210	4	20	theme	4.94×103Da	994:1003	arg1	Mw					972:973	Mw	972:973	Mw	972:973	The results showed that CPP-3 was comprised of two polysaccharides with average molecular weight (Mw) of 5.69×104Da and 4.94×103Da.
29032210	4	20	theme	4.94×103Da	994:1003	arg1	weight					964:969	average molecular weight	946:969	average molecular weight (Mw) of 5.69×104Da and 4.94×103Da	946:1003	The results showed that CPP-3 was comprised of two polysaccharides with average molecular weight (Mw) of 5.69×104Da and 4.94×103Da.
29032210	3	21	theme	red	721:723	arg1	assay					732:736	Neutral red uptake assay	713:736	Neutral red uptake assay	713:736	Cell viability was measured by MTT test, phagocytosis assay was measured by Neutral red uptake assay, nitrite was measured by Griess assay, TNF-α and IL-1β analysis were measured by ELISA, PGE2 was measured by enzyme immunoassay system.
29032210	6	22	theme	macrophage	1281:1290	arg1	RAW264.7					1292:1299	mouse macrophage RAW264.7	1275:1299	mouse macrophage RAW264.7	1275:1299	CPP-3 increased the amount of NO released from mouse macrophage RAW264.7 and significantly increased the levels of TNF-α, IL-1β and PGE2 (P<0.01).
29032210	1	23	theme	polysaccharide	219:232	arg1	composition					184:194	the chemical composition	171:194	the chemical composition of Cyclocarya paliurus polysaccharide and inflammatory effects in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage	171:316	This study was designed to study the chemical composition of Cyclocarya paliurus polysaccharide and inflammatory effects in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage.
29032210	0	24	theme	Chemical	0:7	arg1	composition					9:19	Chemical composition	0:19	Chemical composition of Cyclocarya paliurus polysaccharide and inflammatory effects in lipopolysaccharide-stimulated RAW264.7 macrophage.	0:136	Chemical composition of Cyclocarya paliurus polysaccharide and inflammatory effects in lipopolysaccharide-stimulated RAW264.7 macrophage.
29032210	4	25	theme	5.69×104Da	979:988	arg1	Mw					972:973	Mw	972:973	Mw	972:973	The results showed that CPP-3 was comprised of two polysaccharides with average molecular weight (Mw) of 5.69×104Da and 4.94×103Da.
29032210	4	25	theme	5.69×104Da	979:988	arg1	weight					964:969	average molecular weight	946:969	average molecular weight (Mw) of 5.69×104Da and 4.94×103Da	946:1003	The results showed that CPP-3 was comprised of two polysaccharides with average molecular weight (Mw) of 5.69×104Da and 4.94×103Da.
29032210	2	26	theme	aqueous	344:350	arg1	elution					325:331	A new elution	319:331	A new elution (0.3% NaCl aqueous solution) of Cyclocarya paliurus polysaccharide (CPP-3)	319:406	A new elution (0.3% NaCl aqueous solution) of Cyclocarya paliurus polysaccharide (CPP-3) was characterized by different methods such as fourier transform infrared spectra (FT-IR), UV-vis, gas chromatography-mass spectrometry (GC-MS), high performance gel chromatography (HPGLC) and scanning electron microscopy (SEM).
29032210	2	26	theme	aqueous	344:350	arg1	solution					352:359	0.3% NaCl aqueous solution	334:359	0.3% NaCl aqueous solution	334:359	A new elution (0.3% NaCl aqueous solution) of Cyclocarya paliurus polysaccharide (CPP-3) was characterized by different methods such as fourier transform infrared spectra (FT-IR), UV-vis, gas chromatography-mass spectrometry (GC-MS), high performance gel chromatography (HPGLC) and scanning electron microscopy (SEM).
29032210	0	27	theme	Cyclocarya	24:33	arg1	polysaccharide					44:57	Cyclocarya paliurus polysaccharide	24:57	Cyclocarya paliurus polysaccharide	24:57	Chemical composition of Cyclocarya paliurus polysaccharide and inflammatory effects in lipopolysaccharide-stimulated RAW264.7 macrophage.
29032210	6	28	attach	released	1261:1268	arg1	RAW264.7					1292:1299	mouse macrophage RAW264.7	1275:1299	mouse macrophage RAW264.7	1275:1299	CPP-3 increased the amount of NO released from mouse macrophage RAW264.7 and significantly increased the levels of TNF-α, IL-1β and PGE2 (P<0.01).
29032210	6	28	attach	released	1261:1268	arg2	NO					1258:1259	NO	1258:1259	NO	1258:1259	CPP-3 increased the amount of NO released from mouse macrophage RAW264.7 and significantly increased the levels of TNF-α, IL-1β and PGE2 (P<0.01).
29032210	6	28	attach	released	1261:1268	arg2	amount					1248:1253	the amount	1244:1253	the amount of NO released from mouse macrophage RAW264.7	1244:1299	CPP-3 increased the amount of NO released from mouse macrophage RAW264.7 and significantly increased the levels of TNF-α, IL-1β and PGE2 (P<0.01).
29032210	2	29	theme	NaCl	339:342	arg1	elution					325:331	A new elution	319:331	A new elution (0.3% NaCl aqueous solution) of Cyclocarya paliurus polysaccharide (CPP-3)	319:406	A new elution (0.3% NaCl aqueous solution) of Cyclocarya paliurus polysaccharide (CPP-3) was characterized by different methods such as fourier transform infrared spectra (FT-IR), UV-vis, gas chromatography-mass spectrometry (GC-MS), high performance gel chromatography (HPGLC) and scanning electron microscopy (SEM).
29032210	2	29	theme	NaCl	339:342	arg1	solution					352:359	0.3% NaCl aqueous solution	334:359	0.3% NaCl aqueous solution	334:359	A new elution (0.3% NaCl aqueous solution) of Cyclocarya paliurus polysaccharide (CPP-3) was characterized by different methods such as fourier transform infrared spectra (FT-IR), UV-vis, gas chromatography-mass spectrometry (GC-MS), high performance gel chromatography (HPGLC) and scanning electron microscopy (SEM).
29032210	6	30	theme	PGE2	1360:1363	arg1	levels					1333:1338	the levels	1329:1338	the levels of TNF-α, IL-1β and PGE2 (P<0.01)	1329:1372	CPP-3 increased the amount of NO released from mouse macrophage RAW264.7 and significantly increased the levels of TNF-α, IL-1β and PGE2 (P<0.01).
29032210	1	31	theme	inflammatory	238:249	arg1	effects					251:257	inflammatory effects	238:257	inflammatory effects	238:257	This study was designed to study the chemical composition of Cyclocarya paliurus polysaccharide and inflammatory effects in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage.
29032210	6	32	theme	TNF-α	1343:1347	arg1	levels					1333:1338	the levels	1329:1338	the levels of TNF-α, IL-1β and PGE2 (P<0.01)	1329:1372	CPP-3 increased the amount of NO released from mouse macrophage RAW264.7 and significantly increased the levels of TNF-α, IL-1β and PGE2 (P<0.01).
29032210	2	33	theme	gel	570:572	arg1	HPGLC					590:594	HPGLC	590:594	HPGLC	590:594	A new elution (0.3% NaCl aqueous solution) of Cyclocarya paliurus polysaccharide (CPP-3) was characterized by different methods such as fourier transform infrared spectra (FT-IR), UV-vis, gas chromatography-mass spectrometry (GC-MS), high performance gel chromatography (HPGLC) and scanning electron microscopy (SEM).
29032210	2	33	theme	gel	570:572	arg1	chromatography					574:587	high performance gel chromatography	553:587	high performance gel chromatography (HPGLC)	553:595	A new elution (0.3% NaCl aqueous solution) of Cyclocarya paliurus polysaccharide (CPP-3) was characterized by different methods such as fourier transform infrared spectra (FT-IR), UV-vis, gas chromatography-mass spectrometry (GC-MS), high performance gel chromatography (HPGLC) and scanning electron microscopy (SEM).
29032210	2	34	theme	%	337:337	arg1	elution					325:331	A new elution	319:331	A new elution (0.3% NaCl aqueous solution) of Cyclocarya paliurus polysaccharide (CPP-3)	319:406	A new elution (0.3% NaCl aqueous solution) of Cyclocarya paliurus polysaccharide (CPP-3) was characterized by different methods such as fourier transform infrared spectra (FT-IR), UV-vis, gas chromatography-mass spectrometry (GC-MS), high performance gel chromatography (HPGLC) and scanning electron microscopy (SEM).
29032210	2	34	theme	%	337:337	arg1	solution					352:359	0.3% NaCl aqueous solution	334:359	0.3% NaCl aqueous solution	334:359	A new elution (0.3% NaCl aqueous solution) of Cyclocarya paliurus polysaccharide (CPP-3) was characterized by different methods such as fourier transform infrared spectra (FT-IR), UV-vis, gas chromatography-mass spectrometry (GC-MS), high performance gel chromatography (HPGLC) and scanning electron microscopy (SEM).
29032210	5	35	theme	monosaccharides	1172:1186	arg1	0.060:0.109:0.053:0.128:0.293:0.357					1191:1225	0.060:0.109:0.053:0.128:0.293:0.357	1191:1225	0.060:0.109:0.053:0.128:0.293:0.357	1191:1225	CPP-3 contains six monosaccharides, of which are rhamnose (Rha), arabinose (Ara), xylose (Xyl), mannose (Man), glucose (Glu), galactose (Gal), the molar ratio of six monosaccharides is 0.060:0.109:0.053:0.128:0.293:0.357.
29032210	5	35	theme	monosaccharides	1172:1186	arg1	ratio					1159:1163	the molar ratio	1149:1163	the molar ratio of six monosaccharides	1149:1186	CPP-3 contains six monosaccharides, of which are rhamnose (Rha), arabinose (Ara), xylose (Xyl), mannose (Man), glucose (Glu), galactose (Gal), the molar ratio of six monosaccharides is 0.060:0.109:0.053:0.128:0.293:0.357.
29032210	1	36	theme	effects	251:257	arg1	composition					184:194	the chemical composition	171:194	the chemical composition of Cyclocarya paliurus polysaccharide and inflammatory effects in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage	171:316	This study was designed to study the chemical composition of Cyclocarya paliurus polysaccharide and inflammatory effects in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage.
29032210	0	37	theme	polysaccharide	44:57	arg1	composition					9:19	Chemical composition	0:19	Chemical composition of Cyclocarya paliurus polysaccharide and inflammatory effects in lipopolysaccharide-stimulated RAW264.7 macrophage.	0:136	Chemical composition of Cyclocarya paliurus polysaccharide and inflammatory effects in lipopolysaccharide-stimulated RAW264.7 macrophage.
29032210	8	38	theme	synergistic	1502:1512	arg1	effect					1514:1519	synergistic effect	1502:1519	synergistic effect on the release of NO	1502:1540	CPP-3 and LPS accounted for synergistic effect on the release of NO and TNF-α, CPP-3 and LPS accounted for antagonistic effect on the release of IL-1β and PGE2.
29032210	2	39	theme	performance	558:568	arg1	HPGLC					590:594	HPGLC	590:594	HPGLC	590:594	A new elution (0.3% NaCl aqueous solution) of Cyclocarya paliurus polysaccharide (CPP-3) was characterized by different methods such as fourier transform infrared spectra (FT-IR), UV-vis, gas chromatography-mass spectrometry (GC-MS), high performance gel chromatography (HPGLC) and scanning electron microscopy (SEM).
29032210	2	39	theme	performance	558:568	arg1	chromatography					574:587	high performance gel chromatography	553:587	high performance gel chromatography (HPGLC)	553:595	A new elution (0.3% NaCl aqueous solution) of Cyclocarya paliurus polysaccharide (CPP-3) was characterized by different methods such as fourier transform infrared spectra (FT-IR), UV-vis, gas chromatography-mass spectrometry (GC-MS), high performance gel chromatography (HPGLC) and scanning electron microscopy (SEM).
29032210	8	40	theme	antagonistic	1581:1592	arg1	effect					1594:1599	antagonistic effect	1581:1599	antagonistic effect on the release of IL-1β and PGE2	1581:1632	CPP-3 and LPS accounted for synergistic effect on the release of NO and TNF-α, CPP-3 and LPS accounted for antagonistic effect on the release of IL-1β and PGE2.
29032210	7	41	theme	RAW264.7	1407:1414	arg1	macrophage					1416:1425	LPS-stimulated RAW264.7 macrophage	1392:1425	LPS-stimulated RAW264.7 macrophage	1392:1425	CPP-3 suppressed LPS-stimulated RAW264.7 macrophage to release NO, TNF-α, IL-1β and PGE2 (P<0.01).
29032210	0	42	theme	paliurus	35:42	arg1	polysaccharide					44:57	Cyclocarya paliurus polysaccharide	24:57	Cyclocarya paliurus polysaccharide	24:57	Chemical composition of Cyclocarya paliurus polysaccharide and inflammatory effects in lipopolysaccharide-stimulated RAW264.7 macrophage.
29032210	6	43	theme	mouse	1275:1279	arg1	RAW264.7					1292:1299	mouse macrophage RAW264.7	1275:1299	mouse macrophage RAW264.7	1275:1299	CPP-3 increased the amount of NO released from mouse macrophage RAW264.7 and significantly increased the levels of TNF-α, IL-1β and PGE2 (P<0.01).
29032210	2	44	theme	high	553:556	arg1	HPGLC					590:594	HPGLC	590:594	HPGLC	590:594	A new elution (0.3% NaCl aqueous solution) of Cyclocarya paliurus polysaccharide (CPP-3) was characterized by different methods such as fourier transform infrared spectra (FT-IR), UV-vis, gas chromatography-mass spectrometry (GC-MS), high performance gel chromatography (HPGLC) and scanning electron microscopy (SEM).
29032210	2	44	theme	high	553:556	arg1	chromatography					574:587	high performance gel chromatography	553:587	high performance gel chromatography (HPGLC)	553:595	A new elution (0.3% NaCl aqueous solution) of Cyclocarya paliurus polysaccharide (CPP-3) was characterized by different methods such as fourier transform infrared spectra (FT-IR), UV-vis, gas chromatography-mass spectrometry (GC-MS), high performance gel chromatography (HPGLC) and scanning electron microscopy (SEM).
29032210	2	45	theme	infrared	473:480	arg1	spectra					482:488	infrared spectra	473:488	infrared spectra (FT-IR)	473:496	A new elution (0.3% NaCl aqueous solution) of Cyclocarya paliurus polysaccharide (CPP-3) was characterized by different methods such as fourier transform infrared spectra (FT-IR), UV-vis, gas chromatography-mass spectrometry (GC-MS), high performance gel chromatography (HPGLC) and scanning electron microscopy (SEM).
29032210	2	45	theme	infrared	473:480	arg1	FT-IR					491:495	FT-IR	491:495	FT-IR	491:495	A new elution (0.3% NaCl aqueous solution) of Cyclocarya paliurus polysaccharide (CPP-3) was characterized by different methods such as fourier transform infrared spectra (FT-IR), UV-vis, gas chromatography-mass spectrometry (GC-MS), high performance gel chromatography (HPGLC) and scanning electron microscopy (SEM).
29032210	0	46	theme	inflammatory	63:74	arg1	effects					76:82	inflammatory effects	63:82	inflammatory effects	63:82	Chemical composition of Cyclocarya paliurus polysaccharide and inflammatory effects in lipopolysaccharide-stimulated RAW264.7 macrophage.
29032210	0	47	theme	effects	76:82	arg1	composition					9:19	Chemical composition	0:19	Chemical composition of Cyclocarya paliurus polysaccharide and inflammatory effects in lipopolysaccharide-stimulated RAW264.7 macrophage.	0:136	Chemical composition of Cyclocarya paliurus polysaccharide and inflammatory effects in lipopolysaccharide-stimulated RAW264.7 macrophage.
29032210	2	48	dep	fourier	455:461	arg1	transform					463:471	transform	463:471	transform infrared spectra (FT-IR), UV-vis, gas chromatography-mass spectrometry (GC-MS), high performance gel chromatography (HPGLC) and scanning electron microscopy (SEM)	463:634	A new elution (0.3% NaCl aqueous solution) of Cyclocarya paliurus polysaccharide (CPP-3) was characterized by different methods such as fourier transform infrared spectra (FT-IR), UV-vis, gas chromatography-mass spectrometry (GC-MS), high performance gel chromatography (HPGLC) and scanning electron microscopy (SEM).
29032210	2	49	theme	paliurus	376:383	arg1	CPP-3					401:405	CPP-3	401:405	CPP-3	401:405	A new elution (0.3% NaCl aqueous solution) of Cyclocarya paliurus polysaccharide (CPP-3) was characterized by different methods such as fourier transform infrared spectra (FT-IR), UV-vis, gas chromatography-mass spectrometry (GC-MS), high performance gel chromatography (HPGLC) and scanning electron microscopy (SEM).
29032210	2	49	theme	paliurus	376:383	arg1	polysaccharide					385:398	Cyclocarya paliurus polysaccharide	365:398	Cyclocarya paliurus polysaccharide (CPP-3)	365:406	A new elution (0.3% NaCl aqueous solution) of Cyclocarya paliurus polysaccharide (CPP-3) was characterized by different methods such as fourier transform infrared spectra (FT-IR), UV-vis, gas chromatography-mass spectrometry (GC-MS), high performance gel chromatography (HPGLC) and scanning electron microscopy (SEM).
29032210	5	50	contain	contains	1012:1019	arg2	monosaccharides					1025:1039	six monosaccharides	1021:1039	six monosaccharides	1021:1039	CPP-3 contains six monosaccharides, of which are rhamnose (Rha), arabinose (Ara), xylose (Xyl), mannose (Man), glucose (Glu), galactose (Gal), the molar ratio of six monosaccharides is 0.060:0.109:0.053:0.128:0.293:0.357.
29032210	5	50	contain	contains	1012:1019	arg1	CPP-3					1006:1010	CPP-3	1006:1010	CPP-3	1006:1010	CPP-3 contains six monosaccharides, of which are rhamnose (Rha), arabinose (Ara), xylose (Xyl), mannose (Man), glucose (Glu), galactose (Gal), the molar ratio of six monosaccharides is 0.060:0.109:0.053:0.128:0.293:0.357.
29032210	2	51	theme	different	429:437	arg1	methods					439:445	different methods	429:445	different methods such as fourier transform infrared spectra (FT-IR), UV-vis, gas chromatography-mass spectrometry (GC-MS), high performance gel chromatography (HPGLC) and scanning electron microscopy (SEM)	429:634	A new elution (0.3% NaCl aqueous solution) of Cyclocarya paliurus polysaccharide (CPP-3) was characterized by different methods such as fourier transform infrared spectra (FT-IR), UV-vis, gas chromatography-mass spectrometry (GC-MS), high performance gel chromatography (HPGLC) and scanning electron microscopy (SEM).
29032210	2	51	theme	different	429:437	arg1	fourier					455:461	fourier	455:461	fourier transform infrared spectra (FT-IR), UV-vis, gas chromatography-mass spectrometry (GC-MS), high performance gel chromatography (HPGLC) and scanning electron microscopy (SEM)	455:634	A new elution (0.3% NaCl aqueous solution) of Cyclocarya paliurus polysaccharide (CPP-3) was characterized by different methods such as fourier transform infrared spectra (FT-IR), UV-vis, gas chromatography-mass spectrometry (GC-MS), high performance gel chromatography (HPGLC) and scanning electron microscopy (SEM).
29032210	7	52	theme	LPS-stimulated	1392:1405	arg1	macrophage					1416:1425	LPS-stimulated RAW264.7 macrophage	1392:1425	LPS-stimulated RAW264.7 macrophage	1392:1425	CPP-3 suppressed LPS-stimulated RAW264.7 macrophage to release NO, TNF-α, IL-1β and PGE2 (P<0.01).
29032210	4	53	theme	average	946:952	arg1	Mw					972:973	Mw	972:973	Mw	972:973	The results showed that CPP-3 was comprised of two polysaccharides with average molecular weight (Mw) of 5.69×104Da and 4.94×103Da.
29032210	4	53	theme	average	946:952	arg1	weight					964:969	average molecular weight	946:969	average molecular weight (Mw) of 5.69×104Da and 4.94×103Da	946:1003	The results showed that CPP-3 was comprised of two polysaccharides with average molecular weight (Mw) of 5.69×104Da and 4.94×103Da.
29032210	2	54	theme	Cyclocarya	365:374	arg1	CPP-3					401:405	CPP-3	401:405	CPP-3	401:405	A new elution (0.3% NaCl aqueous solution) of Cyclocarya paliurus polysaccharide (CPP-3) was characterized by different methods such as fourier transform infrared spectra (FT-IR), UV-vis, gas chromatography-mass spectrometry (GC-MS), high performance gel chromatography (HPGLC) and scanning electron microscopy (SEM).
29032210	2	54	theme	Cyclocarya	365:374	arg1	polysaccharide					385:398	Cyclocarya paliurus polysaccharide	365:398	Cyclocarya paliurus polysaccharide (CPP-3)	365:406	A new elution (0.3% NaCl aqueous solution) of Cyclocarya paliurus polysaccharide (CPP-3) was characterized by different methods such as fourier transform infrared spectra (FT-IR), UV-vis, gas chromatography-mass spectrometry (GC-MS), high performance gel chromatography (HPGLC) and scanning electron microscopy (SEM).
29032210	3	55	theme	Griess	763:768	arg1	assay					770:774	Griess assay	763:774	Griess assay	763:774	Cell viability was measured by MTT test, phagocytosis assay was measured by Neutral red uptake assay, nitrite was measured by Griess assay, TNF-α and IL-1β analysis were measured by ELISA, PGE2 was measured by enzyme immunoassay system.
29032210	2	56	dep	spectrometry	531:542	arg1	gas					507:509	UV-vis, gas chromatography-mass spectrometry	499:542	gas	507:509	A new elution (0.3% NaCl aqueous solution) of Cyclocarya paliurus polysaccharide (CPP-3) was characterized by different methods such as fourier transform infrared spectra (FT-IR), UV-vis, gas chromatography-mass spectrometry (GC-MS), high performance gel chromatography (HPGLC) and scanning electron microscopy (SEM).
29032210	3	57	theme	phagocytosis	678:689	arg1	assay					691:695	phagocytosis assay	678:695	phagocytosis assay	678:695	Cell viability was measured by MTT test, phagocytosis assay was measured by Neutral red uptake assay, nitrite was measured by Griess assay, TNF-α and IL-1β analysis were measured by ELISA, PGE2 was measured by enzyme immunoassay system.
29032210	8	58	from	effect	1594:1599	arg1	release					1608:1614	the release	1604:1614	the release of IL-1β and PGE2	1604:1632	CPP-3 and LPS accounted for synergistic effect on the release of NO and TNF-α, CPP-3 and LPS accounted for antagonistic effect on the release of IL-1β and PGE2.
29032210	8	59	theme	IL-1β	1619:1623	arg1	release					1608:1614	the release	1604:1614	the release of IL-1β and PGE2	1604:1632	CPP-3 and LPS accounted for synergistic effect on the release of NO and TNF-α, CPP-3 and LPS accounted for antagonistic effect on the release of IL-1β and PGE2.
29032210	3	60	theme	IL-1β	787:791	arg1	analysis					793:800	IL-1β analysis	787:800	IL-1β analysis	787:800	Cell viability was measured by MTT test, phagocytosis assay was measured by Neutral red uptake assay, nitrite was measured by Griess assay, TNF-α and IL-1β analysis were measured by ELISA, PGE2 was measured by enzyme immunoassay system.
29032210	8	61	from	effect	1514:1519	arg1	release					1528:1534	the release	1524:1534	the release of NO	1524:1540	CPP-3 and LPS accounted for synergistic effect on the release of NO and TNF-α, CPP-3 and LPS accounted for antagonistic effect on the release of IL-1β and PGE2.
29032210	3	62	theme	MTT	668:670	arg1	test					672:675	MTT test	668:675	MTT test	668:675	Cell viability was measured by MTT test, phagocytosis assay was measured by Neutral red uptake assay, nitrite was measured by Griess assay, TNF-α and IL-1β analysis were measured by ELISA, PGE2 was measured by enzyme immunoassay system.
29032210	3	63	theme	Cell	637:640	arg1	viability					642:650	Cell viability	637:650	Cell viability	637:650	Cell viability was measured by MTT test, phagocytosis assay was measured by Neutral red uptake assay, nitrite was measured by Griess assay, TNF-α and IL-1β analysis were measured by ELISA, PGE2 was measured by enzyme immunoassay system.
29032210	5	64	theme	molar	1153:1157	arg1	0.060:0.109:0.053:0.128:0.293:0.357					1191:1225	0.060:0.109:0.053:0.128:0.293:0.357	1191:1225	0.060:0.109:0.053:0.128:0.293:0.357	1191:1225	CPP-3 contains six monosaccharides, of which are rhamnose (Rha), arabinose (Ara), xylose (Xyl), mannose (Man), glucose (Glu), galactose (Gal), the molar ratio of six monosaccharides is 0.060:0.109:0.053:0.128:0.293:0.357.
29032210	5	64	theme	molar	1153:1157	arg1	ratio					1159:1163	the molar ratio	1149:1163	the molar ratio of six monosaccharides	1149:1186	CPP-3 contains six monosaccharides, of which are rhamnose (Rha), arabinose (Ara), xylose (Xyl), mannose (Man), glucose (Glu), galactose (Gal), the molar ratio of six monosaccharides is 0.060:0.109:0.053:0.128:0.293:0.357.
29032210	4	65	theme	molecular	954:962	arg1	Mw					972:973	Mw	972:973	Mw	972:973	The results showed that CPP-3 was comprised of two polysaccharides with average molecular weight (Mw) of 5.69×104Da and 4.94×103Da.
29032210	4	65	theme	molecular	954:962	arg1	weight					964:969	average molecular weight	946:969	average molecular weight (Mw) of 5.69×104Da and 4.94×103Da	946:1003	The results showed that CPP-3 was comprised of two polysaccharides with average molecular weight (Mw) of 5.69×104Da and 4.94×103Da.
29032210	4	66	with	polysaccharides	925:939	arg1	Mw					972:973	Mw	972:973	Mw	972:973	The results showed that CPP-3 was comprised of two polysaccharides with average molecular weight (Mw) of 5.69×104Da and 4.94×103Da.
29032210	4	66	with	polysaccharides	925:939	arg1	weight					964:969	average molecular weight	946:969	average molecular weight (Mw) of 5.69×104Da and 4.94×103Da	946:1003	The results showed that CPP-3 was comprised of two polysaccharides with average molecular weight (Mw) of 5.69×104Da and 4.94×103Da.
29032210	2	67	theme	chromatography-mass	511:529	arg1	GC-MS					545:549	GC-MS	545:549	GC-MS	545:549	A new elution (0.3% NaCl aqueous solution) of Cyclocarya paliurus polysaccharide (CPP-3) was characterized by different methods such as fourier transform infrared spectra (FT-IR), UV-vis, gas chromatography-mass spectrometry (GC-MS), high performance gel chromatography (HPGLC) and scanning electron microscopy (SEM).
29032210	2	67	theme	chromatography-mass	511:529	arg1	spectrometry					531:542	UV-vis, gas chromatography-mass spectrometry	499:542	spectrometry	531:542	A new elution (0.3% NaCl aqueous solution) of Cyclocarya paliurus polysaccharide (CPP-3) was characterized by different methods such as fourier transform infrared spectra (FT-IR), UV-vis, gas chromatography-mass spectrometry (GC-MS), high performance gel chromatography (HPGLC) and scanning electron microscopy (SEM).
29032210	1	68	theme	chemical	175:182	arg1	composition					184:194	the chemical composition	171:194	the chemical composition of Cyclocarya paliurus polysaccharide and inflammatory effects in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage	171:316	This study was designed to study the chemical composition of Cyclocarya paliurus polysaccharide and inflammatory effects in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage.
29032210	3	69	theme	immunoassay	854:864	arg1	system					866:871	enzyme immunoassay system	847:871	enzyme immunoassay system	847:871	Cell viability was measured by MTT test, phagocytosis assay was measured by Neutral red uptake assay, nitrite was measured by Griess assay, TNF-α and IL-1β analysis were measured by ELISA, PGE2 was measured by enzyme immunoassay system.
29032210	1	70	theme	-stimulated	286:296	arg1	macrophage					307:316	lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage	262:316	lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage	262:316	This study was designed to study the chemical composition of Cyclocarya paliurus polysaccharide and inflammatory effects in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage.
28689315	4	0	theme	homochiral	976:985	arg1	D-Trp					990:994	D-Trp	990:994	D-Trp	990:994	In contrast, a photo-induced chemical reaction forming the product ion with m/z 282 occurs in homochiral H+(D-Trp)(D-maltose).
28689315	4	0	theme	homochiral	976:985	arg1	D-maltose					997:1005	D-maltose	997:1005	D-maltose	997:1005	In contrast, a photo-induced chemical reaction forming the product ion with m/z 282 occurs in homochiral H+(D-Trp)(D-maltose).
28689315	4	0	theme	homochiral	976:985	arg1	H+					987:988	homochiral H+	976:988	homochiral H+(D-Trp)(D-maltose)	976:1006	In contrast, a photo-induced chemical reaction forming the product ion with m/z 282 occurs in homochiral H+(D-Trp)(D-maltose).
28689315	0	1	from	Reaction	35:42	arg1	Phase					99:103	the Gas Phase	91:103	the Gas Phase	91:103	Enantiomer-Selective Photo-Induced Reaction of Protonated Tryptophan with Disaccharides in the Gas Phase.
28689315	6	2	gly	C-glycosylation	1202:1216	arg2	complexes					1334:1342	cold gas-phase noncovalent complexes	1307:1342	cold gas-phase noncovalent complexes	1307:1342	The m/z 282 product ion indicates that the photo-induced C-glycosylation, which links D-glucose residues to the indole moiety of Trp via a C-C bond, can occur in cold gas-phase noncovalent complexes, and its enantiomer-selectivity depends on the structure of the disaccharide.
28689315	6	3	theme	gas-phase	1312:1320	arg1	complexes					1334:1342	cold gas-phase noncovalent complexes	1307:1342	cold gas-phase noncovalent complexes	1307:1342	The m/z 282 product ion indicates that the photo-induced C-glycosylation, which links D-glucose residues to the indole moiety of Trp via a C-C bond, can occur in cold gas-phase noncovalent complexes, and its enantiomer-selectivity depends on the structure of the disaccharide.
28689315	3	4	theme	atom	755:758	arg1	transfer					760:767	hydrogen atom transfer	746:767	hydrogen atom transfer from the NH3+ group of a protonated Trp	746:807	NH2CHCOOH loss via cleavage of the Cα-Cβ bond in Trp induced by hydrogen atom transfer from the NH3+ group of a protonated Trp was observed in a noncovalent heterochiral H+(L-Trp)(D-maltose) complex.
28689315	1	5	theme	gas	284:286	arg1	phase					288:292	the gas phase	280:292	the gas phase	280:292	In order to investigate chemical evolution in interstellar molecular clouds, enantiomer-selective photo-induced chemical reactions between an amino acid and disaccharides in the gas phase were examined using a tandem mass spectrometer containing an electrospray ionization source and a cold ion trap.
28689315	0	6	theme	Gas	95:97	arg1	Phase					99:103	the Gas Phase	91:103	the Gas Phase	91:103	Enantiomer-Selective Photo-Induced Reaction of Protonated Tryptophan with Disaccharides in the Gas Phase.
28689315	3	7	theme	bond	723:726	arg1	cleavage					701:708	cleavage	701:708	cleavage of the Cα-Cβ bond in Trp induced by hydrogen atom transfer from the NH3+ group of a protonated Trp	701:807	NH2CHCOOH loss via cleavage of the Cα-Cβ bond in Trp induced by hydrogen atom transfer from the NH3+ group of a protonated Trp was observed in a noncovalent heterochiral H+(L-Trp)(D-maltose) complex.
28689315	3	8	located	observed	813:820	arg1	complex					873:879	a noncovalent heterochiral H+(L-Trp)(D-maltose) complex	825:879	a noncovalent heterochiral H+(L-Trp)(D-maltose) complex	825:879	NH2CHCOOH loss via cleavage of the Cα-Cβ bond in Trp induced by hydrogen atom transfer from the NH3+ group of a protonated Trp was observed in a noncovalent heterochiral H+(L-Trp)(D-maltose) complex.
28689315	3	8	located	observed	813:820	arg2	loss					692:695	NH2CHCOOH loss	682:695	NH2CHCOOH loss via cleavage of the Cα-Cβ bond in Trp induced by hydrogen atom transfer from the NH3+ group of a protonated Trp	682:807	NH2CHCOOH loss via cleavage of the Cα-Cβ bond in Trp induced by hydrogen atom transfer from the NH3+ group of a protonated Trp was observed in a noncovalent heterochiral H+(L-Trp)(D-maltose) complex.
28689315	3	9	from	group	783:787	arg1	transfer					760:767	hydrogen atom transfer	746:767	hydrogen atom transfer from the NH3+ group of a protonated Trp	746:807	NH2CHCOOH loss via cleavage of the Cα-Cβ bond in Trp induced by hydrogen atom transfer from the NH3+ group of a protonated Trp was observed in a noncovalent heterochiral H+(L-Trp)(D-maltose) complex.
28689315	4	10	theme	chemical	911:918	arg1	reaction					920:927	a photo-induced chemical reaction	895:927	a photo-induced chemical reaction forming the product ion with m/z 282	895:964	In contrast, a photo-induced chemical reaction forming the product ion with m/z 282 occurs in homochiral H+(D-Trp)(D-maltose).
28689315	6	11	theme	282	1153:1155	arg1	ion					1165:1167	The m/z 282 product ion	1145:1167	The m/z 282 product ion	1145:1167	The m/z 282 product ion indicates that the photo-induced C-glycosylation, which links D-glucose residues to the indole moiety of Trp via a C-C bond, can occur in cold gas-phase noncovalent complexes, and its enantiomer-selectivity depends on the structure of the disaccharide.
28689315	6	12	theme	disaccharide	1408:1419	arg1	structure					1391:1399	the structure	1387:1399	the structure of the disaccharide	1387:1419	The m/z 282 product ion indicates that the photo-induced C-glycosylation, which links D-glucose residues to the indole moiety of Trp via a C-C bond, can occur in cold gas-phase noncovalent complexes, and its enantiomer-selectivity depends on the structure of the disaccharide.
28689315	3	13	theme	noncovalent	827:837	arg1	complex					873:879	a noncovalent heterochiral H+(L-Trp)(D-maltose) complex	825:879	a noncovalent heterochiral H+(L-Trp)(D-maltose) complex	825:879	NH2CHCOOH loss via cleavage of the Cα-Cβ bond in Trp induced by hydrogen atom transfer from the NH3+ group of a protonated Trp was observed in a noncovalent heterochiral H+(L-Trp)(D-maltose) complex.
28689315	4	14	theme	product	941:947	arg1	ion					949:951	the product ion	937:951	the product ion	937:951	In contrast, a photo-induced chemical reaction forming the product ion with m/z 282 occurs in homochiral H+(D-Trp)(D-maltose).
28689315	1	15	theme	cold	392:395	arg1	trap					401:404	a cold ion trap	390:404	a cold ion trap	390:404	In order to investigate chemical evolution in interstellar molecular clouds, enantiomer-selective photo-induced chemical reactions between an amino acid and disaccharides in the gas phase were examined using a tandem mass spectrometer containing an electrospray ionization source and a cold ion trap.
28689315	1	16	from	acid	254:257	arg1	phase					288:292	the gas phase	280:292	the gas phase	280:292	In order to investigate chemical evolution in interstellar molecular clouds, enantiomer-selective photo-induced chemical reactions between an amino acid and disaccharides in the gas phase were examined using a tandem mass spectrometer containing an electrospray ionization source and a cold ion trap.
28689315	6	17	theme	m/z	1149:1151	arg1	ion					1165:1167	The m/z 282 product ion	1145:1167	The m/z 282 product ion	1145:1167	The m/z 282 product ion indicates that the photo-induced C-glycosylation, which links D-glucose residues to the indole moiety of Trp via a C-C bond, can occur in cold gas-phase noncovalent complexes, and its enantiomer-selectivity depends on the structure of the disaccharide.
28689315	3	18	theme	heterochiral	839:850	arg1	complex					873:879	a noncovalent heterochiral H+(L-Trp)(D-maltose) complex	825:879	a noncovalent heterochiral H+(L-Trp)(D-maltose) complex	825:879	NH2CHCOOH loss via cleavage of the Cα-Cβ bond in Trp induced by hydrogen atom transfer from the NH3+ group of a protonated Trp was observed in a noncovalent heterochiral H+(L-Trp)(D-maltose) complex.
28689315	2	19	theme	D-glucose	570:578	arg1	D-maltose					595:603	D-maltose	595:603	D-maltose	595:603	Ultraviolet photodissociation mass spectra of cold gas-phase noncovalent complexes of protonated tryptophan (Trp) enantiomers with disaccharides consisting of two D-glucose units, such as D-maltose or D-cellobiose, were obtained by photoexcitation of the indole ring of Trp.
28689315	2	19	theme	D-glucose	570:578	arg1	units					580:584	two D-glucose units	566:584	two D-glucose units	566:584	Ultraviolet photodissociation mass spectra of cold gas-phase noncovalent complexes of protonated tryptophan (Trp) enantiomers with disaccharides consisting of two D-glucose units, such as D-maltose or D-cellobiose, were obtained by photoexcitation of the indole ring of Trp.
28689315	2	19	theme	D-glucose	570:578	arg1	D-cellobiose					608:619	D-cellobiose	608:619	D-cellobiose	608:619	Ultraviolet photodissociation mass spectra of cold gas-phase noncovalent complexes of protonated tryptophan (Trp) enantiomers with disaccharides consisting of two D-glucose units, such as D-maltose or D-cellobiose, were obtained by photoexcitation of the indole ring of Trp.
28689315	1	20	theme	ion	397:399	arg1	trap					401:404	a cold ion trap	390:404	a cold ion trap	390:404	In order to investigate chemical evolution in interstellar molecular clouds, enantiomer-selective photo-induced chemical reactions between an amino acid and disaccharides in the gas phase were examined using a tandem mass spectrometer containing an electrospray ionization source and a cold ion trap.
28689315	2	21	theme	Trp	677:679	arg1	ring					669:672	the indole ring	658:672	the indole ring of Trp	658:679	Ultraviolet photodissociation mass spectra of cold gas-phase noncovalent complexes of protonated tryptophan (Trp) enantiomers with disaccharides consisting of two D-glucose units, such as D-maltose or D-cellobiose, were obtained by photoexcitation of the indole ring of Trp.
28689315	2	22	theme	photodissociation	419:435	arg1	spectra					442:448	Ultraviolet photodissociation mass spectra	407:448	Ultraviolet photodissociation mass spectra of cold gas-phase noncovalent complexes of protonated tryptophan (Trp) enantiomers with disaccharides consisting of two D-glucose units, such as D-maltose or D-cellobiose,	407:620	Ultraviolet photodissociation mass spectra of cold gas-phase noncovalent complexes of protonated tryptophan (Trp) enantiomers with disaccharides consisting of two D-glucose units, such as D-maltose or D-cellobiose, were obtained by photoexcitation of the indole ring of Trp.
28689315	1	23	theme	enantiomer-selective	183:202	arg1	reactions					227:235	enantiomer-selective photo-induced chemical reactions	183:235	enantiomer-selective photo-induced chemical reactions between an amino acid and disaccharides in the gas phase	183:292	In order to investigate chemical evolution in interstellar molecular clouds, enantiomer-selective photo-induced chemical reactions between an amino acid and disaccharides in the gas phase were examined using a tandem mass spectrometer containing an electrospray ionization source and a cold ion trap.
28689315	5	24	theme	product	1070:1076	arg1	ion					1078:1080	the m/z 282 product ion	1058:1080	the m/z 282 product ion	1058:1080	For D-cellobiose, both NH2CHCOOH elimination and the m/z 282 product ion were observed, and no enantiomer-selective phenomena occurred.
28689315	2	25	theme	enantiomers	521:531	arg1	complexes					480:488	cold gas-phase noncovalent complexes	453:488	cold gas-phase noncovalent complexes of protonated tryptophan (Trp) enantiomers with disaccharides consisting of two D-glucose units, such as D-maltose or D-cellobiose,	453:620	Ultraviolet photodissociation mass spectra of cold gas-phase noncovalent complexes of protonated tryptophan (Trp) enantiomers with disaccharides consisting of two D-glucose units, such as D-maltose or D-cellobiose, were obtained by photoexcitation of the indole ring of Trp.
28689315	1	26	from	evolution	139:147	arg1	clouds					175:180	interstellar molecular clouds	152:180	interstellar molecular clouds	152:180	In order to investigate chemical evolution in interstellar molecular clouds, enantiomer-selective photo-induced chemical reactions between an amino acid and disaccharides in the gas phase were examined using a tandem mass spectrometer containing an electrospray ionization source and a cold ion trap.
28689315	2	27	theme	tryptophan	504:513	arg1	enantiomers					521:531	protonated tryptophan (Trp) enantiomers	493:531	protonated tryptophan (Trp) enantiomers with disaccharides consisting of two D-glucose units, such as D-maltose or D-cellobiose,	493:620	Ultraviolet photodissociation mass spectra of cold gas-phase noncovalent complexes of protonated tryptophan (Trp) enantiomers with disaccharides consisting of two D-glucose units, such as D-maltose or D-cellobiose, were obtained by photoexcitation of the indole ring of Trp.
28689315	1	28	theme	photo-induced	204:216	arg1	reactions					227:235	enantiomer-selective photo-induced chemical reactions	183:235	enantiomer-selective photo-induced chemical reactions between an amino acid and disaccharides in the gas phase	183:292	In order to investigate chemical evolution in interstellar molecular clouds, enantiomer-selective photo-induced chemical reactions between an amino acid and disaccharides in the gas phase were examined using a tandem mass spectrometer containing an electrospray ionization source and a cold ion trap.
28689315	0	29	theme	Photo-Induced	21:33	arg1	Reaction					35:42	Enantiomer-Selective Photo-Induced Reaction	0:42	Enantiomer-Selective Photo-Induced Reaction of Protonated Tryptophan with Disaccharides in the Gas Phase	0:103	Enantiomer-Selective Photo-Induced Reaction of Protonated Tryptophan with Disaccharides in the Gas Phase.
28689315	3	30	theme	hydrogen	746:753	arg1	transfer					760:767	hydrogen atom transfer	746:767	hydrogen atom transfer from the NH3+ group of a protonated Trp	746:807	NH2CHCOOH loss via cleavage of the Cα-Cβ bond in Trp induced by hydrogen atom transfer from the NH3+ group of a protonated Trp was observed in a noncovalent heterochiral H+(L-Trp)(D-maltose) complex.
28689315	2	31	theme	ring	669:672	arg1	photoexcitation					639:653	photoexcitation	639:653	photoexcitation of the indole ring of Trp	639:679	Ultraviolet photodissociation mass spectra of cold gas-phase noncovalent complexes of protonated tryptophan (Trp) enantiomers with disaccharides consisting of two D-glucose units, such as D-maltose or D-cellobiose, were obtained by photoexcitation of the indole ring of Trp.
28689315	6	32	attach	links	1225:1229	arg2	C-glycosylation					1202:1216	the photo-induced C-glycosylation	1184:1216	the photo-induced C-glycosylation	1184:1216	The m/z 282 product ion indicates that the photo-induced C-glycosylation, which links D-glucose residues to the indole moiety of Trp via a C-C bond, can occur in cold gas-phase noncovalent complexes, and its enantiomer-selectivity depends on the structure of the disaccharide.
28689315	6	32	attach	links	1225:1229	arg1	moiety					1264:1269	the indole moiety	1253:1269	the indole moiety of Trp	1253:1276	The m/z 282 product ion indicates that the photo-induced C-glycosylation, which links D-glucose residues to the indole moiety of Trp via a C-C bond, can occur in cold gas-phase noncovalent complexes, and its enantiomer-selectivity depends on the structure of the disaccharide.
28689315	1	33	theme	chemical	218:225	arg1	reactions					227:235	enantiomer-selective photo-induced chemical reactions	183:235	enantiomer-selective photo-induced chemical reactions between an amino acid and disaccharides in the gas phase	183:292	In order to investigate chemical evolution in interstellar molecular clouds, enantiomer-selective photo-induced chemical reactions between an amino acid and disaccharides in the gas phase were examined using a tandem mass spectrometer containing an electrospray ionization source and a cold ion trap.
28689315	0	34	theme	Enantiomer-Selective	0:19	arg1	Reaction					35:42	Enantiomer-Selective Photo-Induced Reaction	0:42	Enantiomer-Selective Photo-Induced Reaction of Protonated Tryptophan with Disaccharides in the Gas Phase	0:103	Enantiomer-Selective Photo-Induced Reaction of Protonated Tryptophan with Disaccharides in the Gas Phase.
28689315	3	35	theme	NH3+	778:781	arg1	group					783:787	the NH3+ group	774:787	the NH3+ group	774:787	NH2CHCOOH loss via cleavage of the Cα-Cβ bond in Trp induced by hydrogen atom transfer from the NH3+ group of a protonated Trp was observed in a noncovalent heterochiral H+(L-Trp)(D-maltose) complex.
28689315	2	36	theme	indole	662:667	arg1	ring					669:672	the indole ring	658:672	the indole ring of Trp	658:679	Ultraviolet photodissociation mass spectra of cold gas-phase noncovalent complexes of protonated tryptophan (Trp) enantiomers with disaccharides consisting of two D-glucose units, such as D-maltose or D-cellobiose, were obtained by photoexcitation of the indole ring of Trp.
28689315	3	37	from	bond	723:726	arg1	Trp					731:733	Trp	731:733	Trp induced by hydrogen atom transfer from the NH3+ group of a protonated Trp	731:807	NH2CHCOOH loss via cleavage of the Cα-Cβ bond in Trp induced by hydrogen atom transfer from the NH3+ group of a protonated Trp was observed in a noncovalent heterochiral H+(L-Trp)(D-maltose) complex.
28689315	2	38	theme	Ultraviolet	407:417	arg1	spectra					442:448	Ultraviolet photodissociation mass spectra	407:448	Ultraviolet photodissociation mass spectra of cold gas-phase noncovalent complexes of protonated tryptophan (Trp) enantiomers with disaccharides consisting of two D-glucose units, such as D-maltose or D-cellobiose,	407:620	Ultraviolet photodissociation mass spectra of cold gas-phase noncovalent complexes of protonated tryptophan (Trp) enantiomers with disaccharides consisting of two D-glucose units, such as D-maltose or D-cellobiose, were obtained by photoexcitation of the indole ring of Trp.
28689315	6	39	theme	Trp	1274:1276	arg1	moiety					1264:1269	the indole moiety	1253:1269	the indole moiety of Trp	1253:1276	The m/z 282 product ion indicates that the photo-induced C-glycosylation, which links D-glucose residues to the indole moiety of Trp via a C-C bond, can occur in cold gas-phase noncovalent complexes, and its enantiomer-selectivity depends on the structure of the disaccharide.
28689315	6	40	theme	indole	1257:1262	arg1	moiety					1264:1269	the indole moiety	1253:1269	the indole moiety of Trp	1253:1276	The m/z 282 product ion indicates that the photo-induced C-glycosylation, which links D-glucose residues to the indole moiety of Trp via a C-C bond, can occur in cold gas-phase noncovalent complexes, and its enantiomer-selectivity depends on the structure of the disaccharide.
28689315	1	41	contain	containing	341:350	arg2	trap					401:404	a cold ion trap	390:404	a cold ion trap	390:404	In order to investigate chemical evolution in interstellar molecular clouds, enantiomer-selective photo-induced chemical reactions between an amino acid and disaccharides in the gas phase were examined using a tandem mass spectrometer containing an electrospray ionization source and a cold ion trap.
28689315	1	41	contain	containing	341:350	arg2	source					379:384	an electrospray ionization source	352:384	an electrospray ionization source	352:384	In order to investigate chemical evolution in interstellar molecular clouds, enantiomer-selective photo-induced chemical reactions between an amino acid and disaccharides in the gas phase were examined using a tandem mass spectrometer containing an electrospray ionization source and a cold ion trap.
28689315	1	41	contain	containing	341:350	arg1	spectrometer					328:339	a tandem mass spectrometer	314:339	a tandem mass spectrometer containing an electrospray ionization source and a cold ion trap	314:404	In order to investigate chemical evolution in interstellar molecular clouds, enantiomer-selective photo-induced chemical reactions between an amino acid and disaccharides in the gas phase were examined using a tandem mass spectrometer containing an electrospray ionization source and a cold ion trap.
28689315	2	42	theme	noncovalent	468:478	arg1	complexes					480:488	cold gas-phase noncovalent complexes	453:488	cold gas-phase noncovalent complexes of protonated tryptophan (Trp) enantiomers with disaccharides consisting of two D-glucose units, such as D-maltose or D-cellobiose,	453:620	Ultraviolet photodissociation mass spectra of cold gas-phase noncovalent complexes of protonated tryptophan (Trp) enantiomers with disaccharides consisting of two D-glucose units, such as D-maltose or D-cellobiose, were obtained by photoexcitation of the indole ring of Trp.
28689315	2	43	theme	complexes	480:488	arg1	spectra					442:448	Ultraviolet photodissociation mass spectra	407:448	Ultraviolet photodissociation mass spectra of cold gas-phase noncovalent complexes of protonated tryptophan (Trp) enantiomers with disaccharides consisting of two D-glucose units, such as D-maltose or D-cellobiose,	407:620	Ultraviolet photodissociation mass spectra of cold gas-phase noncovalent complexes of protonated tryptophan (Trp) enantiomers with disaccharides consisting of two D-glucose units, such as D-maltose or D-cellobiose, were obtained by photoexcitation of the indole ring of Trp.
28689315	1	44	theme	tandem	316:321	arg1	spectrometer					328:339	a tandem mass spectrometer	314:339	a tandem mass spectrometer containing an electrospray ionization source and a cold ion trap	314:404	In order to investigate chemical evolution in interstellar molecular clouds, enantiomer-selective photo-induced chemical reactions between an amino acid and disaccharides in the gas phase were examined using a tandem mass spectrometer containing an electrospray ionization source and a cold ion trap.
28689315	2	45	theme	protonated	493:502	arg1	enantiomers					521:531	protonated tryptophan (Trp) enantiomers	493:531	protonated tryptophan (Trp) enantiomers with disaccharides consisting of two D-glucose units, such as D-maltose or D-cellobiose,	493:620	Ultraviolet photodissociation mass spectra of cold gas-phase noncovalent complexes of protonated tryptophan (Trp) enantiomers with disaccharides consisting of two D-glucose units, such as D-maltose or D-cellobiose, were obtained by photoexcitation of the indole ring of Trp.
28689315	5	46	theme	NH2CHCOOH	1032:1040	arg1	elimination					1042:1052	NH2CHCOOH elimination	1032:1052	NH2CHCOOH elimination	1032:1052	For D-cellobiose, both NH2CHCOOH elimination and the m/z 282 product ion were observed, and no enantiomer-selective phenomena occurred.
28689315	6	47	theme	cold	1307:1310	arg1	complexes					1334:1342	cold gas-phase noncovalent complexes	1307:1342	cold gas-phase noncovalent complexes	1307:1342	The m/z 282 product ion indicates that the photo-induced C-glycosylation, which links D-glucose residues to the indole moiety of Trp via a C-C bond, can occur in cold gas-phase noncovalent complexes, and its enantiomer-selectivity depends on the structure of the disaccharide.
28689315	2	48	theme	gas-phase	458:466	arg1	complexes					480:488	cold gas-phase noncovalent complexes	453:488	cold gas-phase noncovalent complexes of protonated tryptophan (Trp) enantiomers with disaccharides consisting of two D-glucose units, such as D-maltose or D-cellobiose,	453:620	Ultraviolet photodissociation mass spectra of cold gas-phase noncovalent complexes of protonated tryptophan (Trp) enantiomers with disaccharides consisting of two D-glucose units, such as D-maltose or D-cellobiose, were obtained by photoexcitation of the indole ring of Trp.
28689315	0	49	theme	Tryptophan	58:67	arg1	Reaction					35:42	Enantiomer-Selective Photo-Induced Reaction	0:42	Enantiomer-Selective Photo-Induced Reaction of Protonated Tryptophan with Disaccharides in the Gas Phase	0:103	Enantiomer-Selective Photo-Induced Reaction of Protonated Tryptophan with Disaccharides in the Gas Phase.
28689315	1	50	theme	mass	323:326	arg1	spectrometer					328:339	a tandem mass spectrometer	314:339	a tandem mass spectrometer containing an electrospray ionization source and a cold ion trap	314:404	In order to investigate chemical evolution in interstellar molecular clouds, enantiomer-selective photo-induced chemical reactions between an amino acid and disaccharides in the gas phase were examined using a tandem mass spectrometer containing an electrospray ionization source and a cold ion trap.
28689315	2	51	theme	cold	453:456	arg1	complexes					480:488	cold gas-phase noncovalent complexes	453:488	cold gas-phase noncovalent complexes of protonated tryptophan (Trp) enantiomers with disaccharides consisting of two D-glucose units, such as D-maltose or D-cellobiose,	453:620	Ultraviolet photodissociation mass spectra of cold gas-phase noncovalent complexes of protonated tryptophan (Trp) enantiomers with disaccharides consisting of two D-glucose units, such as D-maltose or D-cellobiose, were obtained by photoexcitation of the indole ring of Trp.
28689315	1	52	theme	amino	248:252	arg1	acid					254:257	an amino acid	245:257	an amino acid	245:257	In order to investigate chemical evolution in interstellar molecular clouds, enantiomer-selective photo-induced chemical reactions between an amino acid and disaccharides in the gas phase were examined using a tandem mass spectrometer containing an electrospray ionization source and a cold ion trap.
28689315	0	53	theme	Protonated	47:56	arg1	Tryptophan					58:67	Protonated Tryptophan	47:67	Protonated Tryptophan	47:67	Enantiomer-Selective Photo-Induced Reaction of Protonated Tryptophan with Disaccharides in the Gas Phase.
28689315	3	54	theme	protonated	794:803	arg1	Trp					805:807	a protonated Trp	792:807	a protonated Trp	792:807	NH2CHCOOH loss via cleavage of the Cα-Cβ bond in Trp induced by hydrogen atom transfer from the NH3+ group of a protonated Trp was observed in a noncovalent heterochiral H+(L-Trp)(D-maltose) complex.
28689315	6	55	theme	C-C	1284:1286	arg1	bond					1288:1291	a C-C bond	1282:1291	a C-C bond	1282:1291	The m/z 282 product ion indicates that the photo-induced C-glycosylation, which links D-glucose residues to the indole moiety of Trp via a C-C bond, can occur in cold gas-phase noncovalent complexes, and its enantiomer-selectivity depends on the structure of the disaccharide.
28689315	3	56	from	Trp	731:733	arg1	cleavage					701:708	cleavage	701:708	cleavage of the Cα-Cβ bond in Trp induced by hydrogen atom transfer from the NH3+ group of a protonated Trp	701:807	NH2CHCOOH loss via cleavage of the Cα-Cβ bond in Trp induced by hydrogen atom transfer from the NH3+ group of a protonated Trp was observed in a noncovalent heterochiral H+(L-Trp)(D-maltose) complex.
28689315	3	57	theme	Trp	805:807	arg1	transfer					760:767	hydrogen atom transfer	746:767	hydrogen atom transfer from the NH3+ group of a protonated Trp	746:807	NH2CHCOOH loss via cleavage of the Cα-Cβ bond in Trp induced by hydrogen atom transfer from the NH3+ group of a protonated Trp was observed in a noncovalent heterochiral H+(L-Trp)(D-maltose) complex.
28689315	5	58	theme	m/z	1062:1064	arg1	ion					1078:1080	the m/z 282 product ion	1058:1080	the m/z 282 product ion	1058:1080	For D-cellobiose, both NH2CHCOOH elimination and the m/z 282 product ion were observed, and no enantiomer-selective phenomena occurred.
28689315	3	59	theme	NH2CHCOOH	682:690	arg1	loss					692:695	NH2CHCOOH loss	682:695	NH2CHCOOH loss via cleavage of the Cα-Cβ bond in Trp induced by hydrogen atom transfer from the NH3+ group of a protonated Trp	682:807	NH2CHCOOH loss via cleavage of the Cα-Cβ bond in Trp induced by hydrogen atom transfer from the NH3+ group of a protonated Trp was observed in a noncovalent heterochiral H+(L-Trp)(D-maltose) complex.
28689315	6	60	theme	photo-induced	1188:1200	arg1	C-glycosylation					1202:1216	the photo-induced C-glycosylation	1184:1216	the photo-induced C-glycosylation	1184:1216	The m/z 282 product ion indicates that the photo-induced C-glycosylation, which links D-glucose residues to the indole moiety of Trp via a C-C bond, can occur in cold gas-phase noncovalent complexes, and its enantiomer-selectivity depends on the structure of the disaccharide.
28689315	0	61	with	Reaction	35:42	arg1	Disaccharides					74:86	Disaccharides	74:86	Disaccharides	74:86	Enantiomer-Selective Photo-Induced Reaction of Protonated Tryptophan with Disaccharides in the Gas Phase.
28689315	6	62	theme	D-glucose	1231:1239	arg1	residues					1241:1248	D-glucose residues	1231:1248	D-glucose residues	1231:1248	The m/z 282 product ion indicates that the photo-induced C-glycosylation, which links D-glucose residues to the indole moiety of Trp via a C-C bond, can occur in cold gas-phase noncovalent complexes, and its enantiomer-selectivity depends on the structure of the disaccharide.
28689315	1	63	theme	chemical	130:137	arg1	evolution					139:147	chemical evolution	130:147	chemical evolution in interstellar molecular clouds	130:180	In order to investigate chemical evolution in interstellar molecular clouds, enantiomer-selective photo-induced chemical reactions between an amino acid and disaccharides in the gas phase were examined using a tandem mass spectrometer containing an electrospray ionization source and a cold ion trap.
28689315	1	64	theme	electrospray	355:366	arg1	source					379:384	an electrospray ionization source	352:384	an electrospray ionization source	352:384	In order to investigate chemical evolution in interstellar molecular clouds, enantiomer-selective photo-induced chemical reactions between an amino acid and disaccharides in the gas phase were examined using a tandem mass spectrometer containing an electrospray ionization source and a cold ion trap.
28689315	4	65	theme	photo-induced	897:909	arg1	reaction					920:927	a photo-induced chemical reaction	895:927	a photo-induced chemical reaction forming the product ion with m/z 282	895:964	In contrast, a photo-induced chemical reaction forming the product ion with m/z 282 occurs in homochiral H+(D-Trp)(D-maltose).
28689315	3	66	theme	Cα-Cβ	717:721	arg1	bond					723:726	the Cα-Cβ bond	713:726	the Cα-Cβ bond in Trp induced by hydrogen atom transfer from the NH3+ group of a protonated Trp	713:807	NH2CHCOOH loss via cleavage of the Cα-Cβ bond in Trp induced by hydrogen atom transfer from the NH3+ group of a protonated Trp was observed in a noncovalent heterochiral H+(L-Trp)(D-maltose) complex.
28689315	3	67	theme	D-maltose	862:870	arg1	complex					873:879	a noncovalent heterochiral H+(L-Trp)(D-maltose) complex	825:879	a noncovalent heterochiral H+(L-Trp)(D-maltose) complex	825:879	NH2CHCOOH loss via cleavage of the Cα-Cβ bond in Trp induced by hydrogen atom transfer from the NH3+ group of a protonated Trp was observed in a noncovalent heterochiral H+(L-Trp)(D-maltose) complex.
28689315	1	68	from	disaccharides	263:275	arg1	phase					288:292	the gas phase	280:292	the gas phase	280:292	In order to investigate chemical evolution in interstellar molecular clouds, enantiomer-selective photo-induced chemical reactions between an amino acid and disaccharides in the gas phase were examined using a tandem mass spectrometer containing an electrospray ionization source and a cold ion trap.
28689315	3	69	theme	H+	852:853	arg1	complex					873:879	a noncovalent heterochiral H+(L-Trp)(D-maltose) complex	825:879	a noncovalent heterochiral H+(L-Trp)(D-maltose) complex	825:879	NH2CHCOOH loss via cleavage of the Cα-Cβ bond in Trp induced by hydrogen atom transfer from the NH3+ group of a protonated Trp was observed in a noncovalent heterochiral H+(L-Trp)(D-maltose) complex.
28689315	3	70	theme	L-Trp	855:859	arg1	complex					873:879	a noncovalent heterochiral H+(L-Trp)(D-maltose) complex	825:879	a noncovalent heterochiral H+(L-Trp)(D-maltose) complex	825:879	NH2CHCOOH loss via cleavage of the Cα-Cβ bond in Trp induced by hydrogen atom transfer from the NH3+ group of a protonated Trp was observed in a noncovalent heterochiral H+(L-Trp)(D-maltose) complex.
28689315	3	71	from	cleavage	701:708	arg1	Trp					731:733	Trp	731:733	Trp induced by hydrogen atom transfer from the NH3+ group of a protonated Trp	731:807	NH2CHCOOH loss via cleavage of the Cα-Cβ bond in Trp induced by hydrogen atom transfer from the NH3+ group of a protonated Trp was observed in a noncovalent heterochiral H+(L-Trp)(D-maltose) complex.
28689315	6	72	theme	noncovalent	1322:1332	arg1	complexes					1334:1342	cold gas-phase noncovalent complexes	1307:1342	cold gas-phase noncovalent complexes	1307:1342	The m/z 282 product ion indicates that the photo-induced C-glycosylation, which links D-glucose residues to the indole moiety of Trp via a C-C bond, can occur in cold gas-phase noncovalent complexes, and its enantiomer-selectivity depends on the structure of the disaccharide.
28689315	1	73	theme	interstellar	152:163	arg1	clouds					175:180	interstellar molecular clouds	152:180	interstellar molecular clouds	152:180	In order to investigate chemical evolution in interstellar molecular clouds, enantiomer-selective photo-induced chemical reactions between an amino acid and disaccharides in the gas phase were examined using a tandem mass spectrometer containing an electrospray ionization source and a cold ion trap.
28689315	2	74	theme	mass	437:440	arg1	spectra					442:448	Ultraviolet photodissociation mass spectra	407:448	Ultraviolet photodissociation mass spectra of cold gas-phase noncovalent complexes of protonated tryptophan (Trp) enantiomers with disaccharides consisting of two D-glucose units, such as D-maltose or D-cellobiose,	407:620	Ultraviolet photodissociation mass spectra of cold gas-phase noncovalent complexes of protonated tryptophan (Trp) enantiomers with disaccharides consisting of two D-glucose units, such as D-maltose or D-cellobiose, were obtained by photoexcitation of the indole ring of Trp.
28689315	1	75	theme	ionization	368:377	arg1	source					379:384	an electrospray ionization source	352:384	an electrospray ionization source	352:384	In order to investigate chemical evolution in interstellar molecular clouds, enantiomer-selective photo-induced chemical reactions between an amino acid and disaccharides in the gas phase were examined using a tandem mass spectrometer containing an electrospray ionization source and a cold ion trap.
28689315	6	76	theme	product	1157:1163	arg1	ion					1165:1167	The m/z 282 product ion	1145:1167	The m/z 282 product ion	1145:1167	The m/z 282 product ion indicates that the photo-induced C-glycosylation, which links D-glucose residues to the indole moiety of Trp via a C-C bond, can occur in cold gas-phase noncovalent complexes, and its enantiomer-selectivity depends on the structure of the disaccharide.
28689315	2	77	with	enantiomers	521:531	arg1	disaccharides					538:550	disaccharides	538:550	disaccharides consisting of two D-glucose units, such as D-maltose or D-cellobiose,	538:620	Ultraviolet photodissociation mass spectra of cold gas-phase noncovalent complexes of protonated tryptophan (Trp) enantiomers with disaccharides consisting of two D-glucose units, such as D-maltose or D-cellobiose, were obtained by photoexcitation of the indole ring of Trp.
28689315	1	78	theme	molecular	165:173	arg1	clouds					175:180	interstellar molecular clouds	152:180	interstellar molecular clouds	152:180	In order to investigate chemical evolution in interstellar molecular clouds, enantiomer-selective photo-induced chemical reactions between an amino acid and disaccharides in the gas phase were examined using a tandem mass spectrometer containing an electrospray ionization source and a cold ion trap.
28689315	5	79	theme	enantiomer-selective	1104:1123	arg1	phenomena					1125:1133	no enantiomer-selective phenomena	1101:1133	no enantiomer-selective phenomena	1101:1133	For D-cellobiose, both NH2CHCOOH elimination and the m/z 282 product ion were observed, and no enantiomer-selective phenomena occurred.
27497089	0	0	theme	supercritical	91:103	arg1	solvents					105:112	different supercritical solvents	81:112	different supercritical solvents	81:112	Hydro-liquefaction of microcrystalline cellulose, xylan and industrial lignin in different supercritical solvents.
27497089	6	1	theme	heating	995:1001	arg1	value					1003:1007	the heating value	991:1007	the heating value increased	991:1017	EA results showed that methanol favored the hydrogenation and deoxygenation, resulting in the heating value increased.
27497089	0	2	theme	different	81:89	arg1	solvents					105:112	different supercritical solvents	81:112	different supercritical solvents	81:112	Hydro-liquefaction of microcrystalline cellulose, xylan and industrial lignin in different supercritical solvents.
27497089	1	3	theme	xylan	177:181	arg1	hydro-liquefaction					144:161	hydro-liquefaction	144:161	hydro-liquefaction of cellulose, xylan, and lignin	144:193	The influences of solvent on hydro-liquefaction of cellulose, xylan, and lignin were investigated using micro-autoclave.
27497089	0	4	from	Hydro-liquefaction	0:17	arg1	solvents					105:112	different supercritical solvents	81:112	different supercritical solvents	81:112	Hydro-liquefaction of microcrystalline cellulose, xylan and industrial lignin in different supercritical solvents.
27497089	3	5	with	miscible	569:576	arg1	materials					587:595	raw materials	583:595	raw materials	583:595	The molecular simulation of interactions between solvents and subcomponents indicated that methanol and ethanol were highly miscible with raw materials.
27497089	4	6	theme	chemical	653:660	arg1	compositions					662:673	the chemical compositions	649:673	the chemical compositions of liquid products	649:692	GC-MS and FT-ICR MS characterization revealed that the chemical compositions of liquid products highly depended on the utilized feedstocks.
27497089	5	7	theme	aromatic	835:842	arg1	compounds					844:852	aromatic compounds	835:852	aromatic compounds	835:852	Esters, ketones, and aldehydes were mainly produced from cellulose and xylan conversion, whereas aromatic compounds were primarily derived from lignin conversion.
27497089	2	8	theme	liquefaction	367:378	arg1	characteristics					380:394	similar liquefaction characteristics	359:394	similar liquefaction characteristics of lignin	359:404	The maximum conversion and bio-oil yield obtained from cellulose and xylan liquefaction were achieved in methanol, whereas similar liquefaction characteristics of lignin were observed in methanol and ethanol.
27497089	3	9	theme	raw	583:585	arg1	materials					587:595	raw materials	583:595	raw materials	583:595	The molecular simulation of interactions between solvents and subcomponents indicated that methanol and ethanol were highly miscible with raw materials.
27497089	3	10	theme	interactions	473:484	arg1	simulation					459:468	The molecular simulation	445:468	The molecular simulation of interactions between solvents and subcomponents	445:519	The molecular simulation of interactions between solvents and subcomponents indicated that methanol and ethanol were highly miscible with raw materials.
27497089	4	11	theme	utilized	717:724	arg1	feedstocks					726:735	the utilized feedstocks	713:735	the utilized feedstocks	713:735	GC-MS and FT-ICR MS characterization revealed that the chemical compositions of liquid products highly depended on the utilized feedstocks.
27497089	4	12	theme	MS	615:616	arg1	characterization					618:633	GC-MS and FT-ICR MS characterization	598:633	GC-MS and FT-ICR MS characterization	598:633	GC-MS and FT-ICR MS characterization revealed that the chemical compositions of liquid products highly depended on the utilized feedstocks.
27497089	1	13	theme	lignin	188:193	arg1	hydro-liquefaction					144:161	hydro-liquefaction	144:161	hydro-liquefaction of cellulose, xylan, and lignin	144:193	The influences of solvent on hydro-liquefaction of cellulose, xylan, and lignin were investigated using micro-autoclave.
27497089	5	14	theme	lignin	882:887	arg1	conversion					889:898	lignin conversion	882:898	lignin conversion	882:898	Esters, ketones, and aldehydes were mainly produced from cellulose and xylan conversion, whereas aromatic compounds were primarily derived from lignin conversion.
27497089	4	15	theme	FT-ICR	608:613	arg1	MS					615:616	FT-ICR MS	608:616	FT-ICR MS	608:616	GC-MS and FT-ICR MS characterization revealed that the chemical compositions of liquid products highly depended on the utilized feedstocks.
27497089	5	16	attach	derived	869:875	arg2	compounds					844:852	aromatic compounds	835:852	aromatic compounds	835:852	Esters, ketones, and aldehydes were mainly produced from cellulose and xylan conversion, whereas aromatic compounds were primarily derived from lignin conversion.
27497089	5	16	attach	derived	869:875	arg1	conversion					889:898	lignin conversion	882:898	lignin conversion	882:898	Esters, ketones, and aldehydes were mainly produced from cellulose and xylan conversion, whereas aromatic compounds were primarily derived from lignin conversion.
27497089	0	17	theme	cellulose	39:47	arg1	Hydro-liquefaction					0:17	Hydro-liquefaction	0:17	Hydro-liquefaction of microcrystalline cellulose, xylan and industrial lignin in different supercritical solvents.	0:113	Hydro-liquefaction of microcrystalline cellulose, xylan and industrial lignin in different supercritical solvents.
27497089	0	18	theme	microcrystalline	22:37	arg1	cellulose					39:47	microcrystalline cellulose	22:47	microcrystalline cellulose	22:47	Hydro-liquefaction of microcrystalline cellulose, xylan and industrial lignin in different supercritical solvents.
27497089	0	19	theme	xylan	50:54	arg1	Hydro-liquefaction					0:17	Hydro-liquefaction	0:17	Hydro-liquefaction of microcrystalline cellulose, xylan and industrial lignin in different supercritical solvents.	0:113	Hydro-liquefaction of microcrystalline cellulose, xylan and industrial lignin in different supercritical solvents.
27497089	2	20	theme	similar	359:365	arg1	characteristics					380:394	similar liquefaction characteristics	359:394	similar liquefaction characteristics of lignin	359:404	The maximum conversion and bio-oil yield obtained from cellulose and xylan liquefaction were achieved in methanol, whereas similar liquefaction characteristics of lignin were observed in methanol and ethanol.
27497089	3	21	theme	molecular	449:457	arg1	simulation					459:468	The molecular simulation	445:468	The molecular simulation of interactions between solvents and subcomponents	445:519	The molecular simulation of interactions between solvents and subcomponents indicated that methanol and ethanol were highly miscible with raw materials.
27497089	7	22	theme	supercritical	1086:1098	arg1	methanol					1100:1107	supercritical methanol	1086:1107	supercritical methanol	1086:1107	It could be concluded that the oil quality was highly improved in supercritical methanol.
27497089	0	23	theme	industrial	60:69	arg1	lignin					71:76	industrial lignin	60:76	industrial lignin	60:76	Hydro-liquefaction of microcrystalline cellulose, xylan and industrial lignin in different supercritical solvents.
27497089	2	24	theme	maximum	240:246	arg1	conversion					248:257	The maximum conversion and bio-oil yield	236:275	conversion	248:257	The maximum conversion and bio-oil yield obtained from cellulose and xylan liquefaction were achieved in methanol, whereas similar liquefaction characteristics of lignin were observed in methanol and ethanol.
27497089	2	25	dep	achieved	329:336	arg1	whereas					351:357	whereas	351:357	whereas	351:357	The maximum conversion and bio-oil yield obtained from cellulose and xylan liquefaction were achieved in methanol, whereas similar liquefaction characteristics of lignin were observed in methanol and ethanol.
27497089	4	26	theme	GC-MS	598:602	arg1	characterization					618:633	GC-MS and FT-ICR MS characterization	598:633	GC-MS and FT-ICR MS characterization	598:633	GC-MS and FT-ICR MS characterization revealed that the chemical compositions of liquid products highly depended on the utilized feedstocks.
27497089	1	27	theme	cellulose	166:174	arg1	hydro-liquefaction					144:161	hydro-liquefaction	144:161	hydro-liquefaction of cellulose, xylan, and lignin	144:193	The influences of solvent on hydro-liquefaction of cellulose, xylan, and lignin were investigated using micro-autoclave.
27497089	1	28	theme	solvent	133:139	arg1	influences					119:128	The influences	115:128	The influences of solvent on hydro-liquefaction of cellulose, xylan, and lignin	115:193	The influences of solvent on hydro-liquefaction of cellulose, xylan, and lignin were investigated using micro-autoclave.
27497089	1	29	from	influences	119:128	arg1	hydro-liquefaction					144:161	hydro-liquefaction	144:161	hydro-liquefaction of cellulose, xylan, and lignin	144:193	The influences of solvent on hydro-liquefaction of cellulose, xylan, and lignin were investigated using micro-autoclave.
27497089	5	30	dep	cellulose	795:803	arg1	conversion					815:824	conversion	815:824	conversion	815:824	Esters, ketones, and aldehydes were mainly produced from cellulose and xylan conversion, whereas aromatic compounds were primarily derived from lignin conversion.
27497089	2	31	located	observed	411:418	arg1	methanol					423:430	methanol	423:430	methanol	423:430	The maximum conversion and bio-oil yield obtained from cellulose and xylan liquefaction were achieved in methanol, whereas similar liquefaction characteristics of lignin were observed in methanol and ethanol.
27497089	2	31	located	observed	411:418	arg2	characteristics					380:394	similar liquefaction characteristics	359:394	similar liquefaction characteristics of lignin	359:404	The maximum conversion and bio-oil yield obtained from cellulose and xylan liquefaction were achieved in methanol, whereas similar liquefaction characteristics of lignin were observed in methanol and ethanol.
27497089	2	31	located	observed	411:418	arg1	ethanol					436:442	ethanol	436:442	ethanol	436:442	The maximum conversion and bio-oil yield obtained from cellulose and xylan liquefaction were achieved in methanol, whereas similar liquefaction characteristics of lignin were observed in methanol and ethanol.
27497089	2	32	dep	cellulose	291:299	arg1	liquefaction					311:322	liquefaction	311:322	liquefaction	311:322	The maximum conversion and bio-oil yield obtained from cellulose and xylan liquefaction were achieved in methanol, whereas similar liquefaction characteristics of lignin were observed in methanol and ethanol.
27497089	6	33	theme	EA	901:902	arg1	results					904:910	EA results	901:910	EA results	901:910	EA results showed that methanol favored the hydrogenation and deoxygenation, resulting in the heating value increased.
27497089	4	34	theme	products	685:692	arg1	compositions					662:673	the chemical compositions	649:673	the chemical compositions of liquid products	649:692	GC-MS and FT-ICR MS characterization revealed that the chemical compositions of liquid products highly depended on the utilized feedstocks.
27497089	0	35	theme	lignin	71:76	arg1	Hydro-liquefaction					0:17	Hydro-liquefaction	0:17	Hydro-liquefaction of microcrystalline cellulose, xylan and industrial lignin in different supercritical solvents.	0:113	Hydro-liquefaction of microcrystalline cellulose, xylan and industrial lignin in different supercritical solvents.
27497089	2	36	theme	bio-oil	263:269	arg1	yield					271:275	The maximum conversion and bio-oil yield	236:275	yield	271:275	The maximum conversion and bio-oil yield obtained from cellulose and xylan liquefaction were achieved in methanol, whereas similar liquefaction characteristics of lignin were observed in methanol and ethanol.
27497089	7	37	theme	oil	1051:1053	arg1	quality					1055:1061	the oil quality	1047:1061	the oil quality	1047:1061	It could be concluded that the oil quality was highly improved in supercritical methanol.
27497089	2	38	theme	lignin	399:404	arg1	characteristics					380:394	similar liquefaction characteristics	359:394	similar liquefaction characteristics of lignin	359:404	The maximum conversion and bio-oil yield obtained from cellulose and xylan liquefaction were achieved in methanol, whereas similar liquefaction characteristics of lignin were observed in methanol and ethanol.
27497089	4	39	theme	liquid	678:683	arg1	products					685:692	liquid products	678:692	liquid products	678:692	GC-MS and FT-ICR MS characterization revealed that the chemical compositions of liquid products highly depended on the utilized feedstocks.
29055533	6	0	from	thermophilus	1013:1024	arg1	mass					993:996	the molecular mass	979:996	the molecular mass of EPS from S. thermophilus KLDS SM cultivated under different carbon sources	979:1074	Furthermore, differences in the molecular mass of EPS from S. thermophilus KLDS SM cultivated under different carbon sources were correlated with the transcription levels of the genes encoding chain length determination protein and glycosyltransferase.
29055533	6	0	from	thermophilus	1013:1024	arg1	EPS					1001:1003	EPS	1001:1003	EPS from S. thermophilus KLDS SM cultivated under different carbon sources	1001:1074	Furthermore, differences in the molecular mass of EPS from S. thermophilus KLDS SM cultivated under different carbon sources were correlated with the transcription levels of the genes encoding chain length determination protein and glycosyltransferase.
29055533	3	1	theme	nucleotide	509:518	arg1	sugars					520:525	nucleotide sugars	509:525	nucleotide sugars synthesis	509:535	To understand the possible link between the genotype and the phenotype regarding EPS, the complete genome of S. thermophilus KLDS SM was sequenced and investigated in silico for genes related to carbohydrate fermentation, nucleotide sugars synthesis, and EPS gene cluster.
29055533	4	2	theme	thermophilus	577:588	arg1	SM					595:596	S. thermophilus KLDS SM	574:596	S. thermophilus KLDS SM	574:596	We found that S. thermophilus KLDS SM is able to ferment sucrose, mannose, glucose, galactose, and lactose from the genomic research, which was confirmed by API 50 CH (bioMérieux, Marcy l'Etoile, France).
29055533	6	3	from	mass	993:996	arg1	thermophilus					1013:1024	S. thermophilus	1010:1024	S. thermophilus KLDS SM cultivated under different carbon sources	1010:1074	Furthermore, differences in the molecular mass of EPS from S. thermophilus KLDS SM cultivated under different carbon sources were correlated with the transcription levels of the genes encoding chain length determination protein and glycosyltransferase.
29055533	3	4	theme	sugars	520:525	arg1	synthesis					527:535	nucleotide sugars synthesis	509:535	nucleotide sugars synthesis	509:535	To understand the possible link between the genotype and the phenotype regarding EPS, the complete genome of S. thermophilus KLDS SM was sequenced and investigated in silico for genes related to carbohydrate fermentation, nucleotide sugars synthesis, and EPS gene cluster.
29055533	4	5	theme	S.	574:575	arg1	SM					595:596	S. thermophilus KLDS SM	574:596	S. thermophilus KLDS SM	574:596	We found that S. thermophilus KLDS SM is able to ferment sucrose, mannose, glucose, galactose, and lactose from the genomic research, which was confirmed by API 50 CH (bioMérieux, Marcy l'Etoile, France).
29055533	7	6	theme	conformation	1306:1317	arg1	understanding					1234:1246	a better understanding	1225:1246	a better understanding of the link between the genetic elements and the chemical conformation of EPS	1225:1324	Our findings provide a better understanding of the link between the genetic elements and the chemical conformation of EPS and a theoretical basis for producing tailor-made EPS through genetic and metabolic engineering approaches.
29055533	7	6	theme	conformation	1306:1317	arg1	basis					1344:1348	a theoretical basis	1330:1348	a theoretical basis for producing tailor-made EPS through genetic and metabolic engineering approaches	1330:1431	Our findings provide a better understanding of the link between the genetic elements and the chemical conformation of EPS and a theoretical basis for producing tailor-made EPS through genetic and metabolic engineering approaches.
29055533	6	7	theme	length	1150:1155	arg1	determination					1157:1169	chain length determination	1144:1169	chain length determination protein	1144:1177	Furthermore, differences in the molecular mass of EPS from S. thermophilus KLDS SM cultivated under different carbon sources were correlated with the transcription levels of the genes encoding chain length determination protein and glycosyltransferase.
29055533	3	8	theme	gene	546:549	arg1	cluster					551:557	EPS gene cluster	542:557	EPS gene cluster	542:557	To understand the possible link between the genotype and the phenotype regarding EPS, the complete genome of S. thermophilus KLDS SM was sequenced and investigated in silico for genes related to carbohydrate fermentation, nucleotide sugars synthesis, and EPS gene cluster.
29055533	6	9	theme	carbon	1061:1066	arg1	sources					1068:1074	different carbon sources	1051:1074	different carbon sources	1051:1074	Furthermore, differences in the molecular mass of EPS from S. thermophilus KLDS SM cultivated under different carbon sources were correlated with the transcription levels of the genes encoding chain length determination protein and glycosyltransferase.
29055533	7	10	theme	engineering	1410:1420	arg1	approaches					1422:1431	genetic and metabolic engineering approaches	1388:1431	genetic and metabolic engineering approaches	1388:1431	Our findings provide a better understanding of the link between the genetic elements and the chemical conformation of EPS and a theoretical basis for producing tailor-made EPS through genetic and metabolic engineering approaches.
29055533	6	11	theme	transcription	1101:1113	arg1	levels					1115:1120	the transcription levels	1097:1120	the transcription levels of the genes encoding chain length determination protein and glycosyltransferase	1097:1201	Furthermore, differences in the molecular mass of EPS from S. thermophilus KLDS SM cultivated under different carbon sources were correlated with the transcription levels of the genes encoding chain length determination protein and glycosyltransferase.
29055533	4	12	dep	CH	724:725	arg1	France					756:761	France	756:761	France	756:761	We found that S. thermophilus KLDS SM is able to ferment sucrose, mannose, glucose, galactose, and lactose from the genomic research, which was confirmed by API 50 CH (bioMérieux, Marcy l'Etoile, France).
29055533	4	12	dep	CH	724:725	arg1	Etoile					748:753	Marcy l'Etoile	740:753	Marcy l'Etoile	740:753	We found that S. thermophilus KLDS SM is able to ferment sucrose, mannose, glucose, galactose, and lactose from the genomic research, which was confirmed by API 50 CH (bioMérieux, Marcy l'Etoile, France).
29055533	4	12	dep	CH	724:725	arg1	bioMérieux					728:737	bioMérieux	728:737	bioMérieux	728:737	We found that S. thermophilus KLDS SM is able to ferment sucrose, mannose, glucose, galactose, and lactose from the genomic research, which was confirmed by API 50 CH (bioMérieux, Marcy l'Etoile, France).
29055533	3	13	theme	carbohydrate	482:493	arg1	fermentation					495:506	carbohydrate fermentation	482:506	carbohydrate fermentation	482:506	To understand the possible link between the genotype and the phenotype regarding EPS, the complete genome of S. thermophilus KLDS SM was sequenced and investigated in silico for genes related to carbohydrate fermentation, nucleotide sugars synthesis, and EPS gene cluster.
29055533	6	14	theme	different	1051:1059	arg1	sources					1068:1074	different carbon sources	1051:1074	different carbon sources	1051:1074	Furthermore, differences in the molecular mass of EPS from S. thermophilus KLDS SM cultivated under different carbon sources were correlated with the transcription levels of the genes encoding chain length determination protein and glycosyltransferase.
29055533	5	15	theme	genetic	769:775	arg1	analysis					777:784	The genetic analysis	765:784	The genetic analysis of nucleotide sugars and EPS cluster	765:821	The genetic analysis of nucleotide sugars and EPS cluster revealed that the EPS produced by this strain are composed of galactose and glucose, in accordance with the biochemical result.
29055533	7	16	theme	tailor-made	1364:1374	arg1	EPS					1376:1378	tailor-made EPS	1364:1378	tailor-made EPS	1364:1378	Our findings provide a better understanding of the link between the genetic elements and the chemical conformation of EPS and a theoretical basis for producing tailor-made EPS through genetic and metabolic engineering approaches.
29055533	5	17	theme	nucleotide	789:798	arg1	sugars					800:805	nucleotide sugars	789:805	nucleotide sugars	789:805	The genetic analysis of nucleotide sugars and EPS cluster revealed that the EPS produced by this strain are composed of galactose and glucose, in accordance with the biochemical result.
29055533	4	18	theme	KLDS	590:593	arg1	SM					595:596	S. thermophilus KLDS SM	574:596	S. thermophilus KLDS SM	574:596	We found that S. thermophilus KLDS SM is able to ferment sucrose, mannose, glucose, galactose, and lactose from the genomic research, which was confirmed by API 50 CH (bioMérieux, Marcy l'Etoile, France).
29055533	0	19	theme	phenotypic	33:42	arg1	analyses					44:51	Genomic and phenotypic analyses	21:51	Short communication: Genomic and phenotypic analyses of exopolysaccharides produced by Streptococcus thermophilus	0:112	Short communication: Genomic and phenotypic analyses of exopolysaccharides produced by Streptococcus thermophilus KLDS SM.
29055533	6	20	from	differences	964:974	arg1	mass					993:996	the molecular mass	979:996	the molecular mass of EPS from S. thermophilus KLDS SM cultivated under different carbon sources	979:1074	Furthermore, differences in the molecular mass of EPS from S. thermophilus KLDS SM cultivated under different carbon sources were correlated with the transcription levels of the genes encoding chain length determination protein and glycosyltransferase.
29055533	0	21	theme	Short	0:4	arg1	communication					6:18	Short communication	0:18	Short communication: Genomic and phenotypic analyses of exopolysaccharides produced by Streptococcus thermophilus	0:112	Short communication: Genomic and phenotypic analyses of exopolysaccharides produced by Streptococcus thermophilus KLDS SM.
29055533	5	22	theme	sugars	800:805	arg1	analysis					777:784	The genetic analysis	765:784	The genetic analysis of nucleotide sugars and EPS cluster	765:821	The genetic analysis of nucleotide sugars and EPS cluster revealed that the EPS produced by this strain are composed of galactose and glucose, in accordance with the biochemical result.
29055533	7	23	theme	metabolic	1400:1408	arg1	approaches					1422:1431	genetic and metabolic engineering approaches	1388:1431	genetic and metabolic engineering approaches	1388:1431	Our findings provide a better understanding of the link between the genetic elements and the chemical conformation of EPS and a theoretical basis for producing tailor-made EPS through genetic and metabolic engineering approaches.
29055533	7	24	theme	link	1255:1258	arg1	understanding					1234:1246	a better understanding	1225:1246	a better understanding of the link between the genetic elements and the chemical conformation of EPS	1225:1324	Our findings provide a better understanding of the link between the genetic elements and the chemical conformation of EPS and a theoretical basis for producing tailor-made EPS through genetic and metabolic engineering approaches.
29055533	7	24	theme	link	1255:1258	arg1	basis					1344:1348	a theoretical basis	1330:1348	a theoretical basis for producing tailor-made EPS through genetic and metabolic engineering approaches	1330:1431	Our findings provide a better understanding of the link between the genetic elements and the chemical conformation of EPS and a theoretical basis for producing tailor-made EPS through genetic and metabolic engineering approaches.
29055533	3	25	theme	S.	396:397	arg1	SM					417:418	S. thermophilus KLDS SM	396:418	S. thermophilus KLDS SM	396:418	To understand the possible link between the genotype and the phenotype regarding EPS, the complete genome of S. thermophilus KLDS SM was sequenced and investigated in silico for genes related to carbohydrate fermentation, nucleotide sugars synthesis, and EPS gene cluster.
29055533	5	26	theme	EPS	811:813	arg1	cluster					815:821	EPS cluster	811:821	EPS cluster	811:821	The genetic analysis of nucleotide sugars and EPS cluster revealed that the EPS produced by this strain are composed of galactose and glucose, in accordance with the biochemical result.
29055533	0	27	theme	Genomic	21:27	arg1	analyses					44:51	Genomic and phenotypic analyses	21:51	Short communication: Genomic and phenotypic analyses of exopolysaccharides produced by Streptococcus thermophilus	0:112	Short communication: Genomic and phenotypic analyses of exopolysaccharides produced by Streptococcus thermophilus KLDS SM.
29055533	3	28	dep	genotype	331:338	arg1	the					327:329	the	327:329	the	327:329	To understand the possible link between the genotype and the phenotype regarding EPS, the complete genome of S. thermophilus KLDS SM was sequenced and investigated in silico for genes related to carbohydrate fermentation, nucleotide sugars synthesis, and EPS gene cluster.
29055533	6	29	dep	thermophilus	1013:1024	arg1	SM					1031:1032	KLDS SM	1026:1032	S. thermophilus KLDS SM cultivated under different carbon sources	1010:1074	Furthermore, differences in the molecular mass of EPS from S. thermophilus KLDS SM cultivated under different carbon sources were correlated with the transcription levels of the genes encoding chain length determination protein and glycosyltransferase.
29055533	4	30	theme	API	717:719	arg1	CH					724:725	API 50 CH	717:725	API 50 CH (bioMérieux, Marcy l'Etoile, France)	717:762	We found that S. thermophilus KLDS SM is able to ferment sucrose, mannose, glucose, galactose, and lactose from the genomic research, which was confirmed by API 50 CH (bioMérieux, Marcy l'Etoile, France).
29055533	2	31	theme	thermophilus	209:220	arg1	SM					227:228	Streptococcus thermophilus KLDS SM	195:228	Streptococcus thermophilus KLDS SM	195:228	Streptococcus thermophilus KLDS SM could produce a high amount of exopolysaccharides (EPS).
29055533	1	32	theme	dairy	179:183	arg1	industry					185:192	the dairy industry	175:192	the dairy industry	175:192	Streptococcus thermophilus plays important roles in the dairy industry.
29055533	3	33	theme	related	471:477	arg1	genes					465:469	genes	465:469	genes related to carbohydrate fermentation, nucleotide sugars synthesis, and EPS gene cluster	465:557	To understand the possible link between the genotype and the phenotype regarding EPS, the complete genome of S. thermophilus KLDS SM was sequenced and investigated in silico for genes related to carbohydrate fermentation, nucleotide sugars synthesis, and EPS gene cluster.
29055533	3	34	theme	SM	417:418	arg1	genome					386:391	the complete genome	373:391	the complete genome of S. thermophilus KLDS SM	373:418	To understand the possible link between the genotype and the phenotype regarding EPS, the complete genome of S. thermophilus KLDS SM was sequenced and investigated in silico for genes related to carbohydrate fermentation, nucleotide sugars synthesis, and EPS gene cluster.
29055533	7	35	theme	better	1227:1232	arg1	understanding					1234:1246	a better understanding	1225:1246	a better understanding of the link between the genetic elements and the chemical conformation of EPS	1225:1324	Our findings provide a better understanding of the link between the genetic elements and the chemical conformation of EPS and a theoretical basis for producing tailor-made EPS through genetic and metabolic engineering approaches.
29055533	2	36	theme	Streptococcus	195:207	arg1	SM					227:228	Streptococcus thermophilus KLDS SM	195:228	Streptococcus thermophilus KLDS SM	195:228	Streptococcus thermophilus KLDS SM could produce a high amount of exopolysaccharides (EPS).
29055533	5	37	theme	biochemical	931:941	arg1	result					943:948	the biochemical result	927:948	the biochemical result	927:948	The genetic analysis of nucleotide sugars and EPS cluster revealed that the EPS produced by this strain are composed of galactose and glucose, in accordance with the biochemical result.
29055533	6	38	theme	molecular	983:991	arg1	mass					993:996	the molecular mass	979:996	the molecular mass of EPS from S. thermophilus KLDS SM cultivated under different carbon sources	979:1074	Furthermore, differences in the molecular mass of EPS from S. thermophilus KLDS SM cultivated under different carbon sources were correlated with the transcription levels of the genes encoding chain length determination protein and glycosyltransferase.
29055533	7	39	theme	genetic	1388:1394	arg1	approaches					1422:1431	genetic and metabolic engineering approaches	1388:1431	genetic and metabolic engineering approaches	1388:1431	Our findings provide a better understanding of the link between the genetic elements and the chemical conformation of EPS and a theoretical basis for producing tailor-made EPS through genetic and metabolic engineering approaches.
29055533	6	40	theme	EPS	1001:1003	arg1	mass					993:996	the molecular mass	979:996	the molecular mass of EPS from S. thermophilus KLDS SM cultivated under different carbon sources	979:1074	Furthermore, differences in the molecular mass of EPS from S. thermophilus KLDS SM cultivated under different carbon sources were correlated with the transcription levels of the genes encoding chain length determination protein and glycosyltransferase.
29055533	7	41	theme	genetic	1272:1278	arg1	elements					1280:1287	the genetic elements	1268:1287	the genetic elements	1268:1287	Our findings provide a better understanding of the link between the genetic elements and the chemical conformation of EPS and a theoretical basis for producing tailor-made EPS through genetic and metabolic engineering approaches.
29055533	3	42	theme	complete	377:384	arg1	genome					386:391	the complete genome	373:391	the complete genome of S. thermophilus KLDS SM	373:418	To understand the possible link between the genotype and the phenotype regarding EPS, the complete genome of S. thermophilus KLDS SM was sequenced and investigated in silico for genes related to carbohydrate fermentation, nucleotide sugars synthesis, and EPS gene cluster.
29055533	7	43	theme	EPS	1322:1324	arg1	link					1255:1258	the link	1251:1258	the link between the genetic elements	1251:1287	Our findings provide a better understanding of the link between the genetic elements and the chemical conformation of EPS and a theoretical basis for producing tailor-made EPS through genetic and metabolic engineering approaches.
29055533	7	43	theme	EPS	1322:1324	arg1	conformation					1306:1317	the chemical conformation	1293:1317	the chemical conformation of EPS	1293:1324	Our findings provide a better understanding of the link between the genetic elements and the chemical conformation of EPS and a theoretical basis for producing tailor-made EPS through genetic and metabolic engineering approaches.
29055533	3	44	theme	thermophilus	399:410	arg1	SM					417:418	S. thermophilus KLDS SM	396:418	S. thermophilus KLDS SM	396:418	To understand the possible link between the genotype and the phenotype regarding EPS, the complete genome of S. thermophilus KLDS SM was sequenced and investigated in silico for genes related to carbohydrate fermentation, nucleotide sugars synthesis, and EPS gene cluster.
29055533	2	45	theme	high	246:249	arg1	amount					251:256	a high amount	244:256	a high amount of exopolysaccharides (EPS)	244:284	Streptococcus thermophilus KLDS SM could produce a high amount of exopolysaccharides (EPS).
29055533	2	45	theme	high	246:249	arg1	EPS					281:283	EPS	281:283	EPS	281:283	Streptococcus thermophilus KLDS SM could produce a high amount of exopolysaccharides (EPS).
29055533	2	45	theme	high	246:249	arg1	exopolysaccharides					261:278	exopolysaccharides	261:278	exopolysaccharides (EPS)	261:284	Streptococcus thermophilus KLDS SM could produce a high amount of exopolysaccharides (EPS).
29055533	6	46	theme	KLDS	1026:1029	arg1	SM					1031:1032	KLDS SM	1026:1032	S. thermophilus KLDS SM cultivated under different carbon sources	1010:1074	Furthermore, differences in the molecular mass of EPS from S. thermophilus KLDS SM cultivated under different carbon sources were correlated with the transcription levels of the genes encoding chain length determination protein and glycosyltransferase.
29055533	1	47	theme	Streptococcus	123:135	arg1	thermophilus					137:148	Streptococcus thermophilus	123:148	Streptococcus thermophilus	123:148	Streptococcus thermophilus plays important roles in the dairy industry.
29055533	3	48	theme	KLDS	412:415	arg1	SM					417:418	S. thermophilus KLDS SM	396:418	S. thermophilus KLDS SM	396:418	To understand the possible link between the genotype and the phenotype regarding EPS, the complete genome of S. thermophilus KLDS SM was sequenced and investigated in silico for genes related to carbohydrate fermentation, nucleotide sugars synthesis, and EPS gene cluster.
29055533	6	49	theme	chain	1144:1148	arg1	determination					1157:1169	chain length determination	1144:1169	chain length determination protein	1144:1177	Furthermore, differences in the molecular mass of EPS from S. thermophilus KLDS SM cultivated under different carbon sources were correlated with the transcription levels of the genes encoding chain length determination protein and glycosyltransferase.
29055533	4	50	theme	genomic	676:682	arg1	research					684:691	the genomic research	672:691	the genomic research	672:691	We found that S. thermophilus KLDS SM is able to ferment sucrose, mannose, glucose, galactose, and lactose from the genomic research, which was confirmed by API 50 CH (bioMérieux, Marcy l'Etoile, France).
29055533	4	51	theme	l'	746:747	arg1	Etoile					748:753	Marcy l'Etoile	740:753	Marcy l'Etoile	740:753	We found that S. thermophilus KLDS SM is able to ferment sucrose, mannose, glucose, galactose, and lactose from the genomic research, which was confirmed by API 50 CH (bioMérieux, Marcy l'Etoile, France).
29055533	4	51	theme	l'	746:747	arg1	bioMérieux					728:737	bioMérieux	728:737	bioMérieux	728:737	We found that S. thermophilus KLDS SM is able to ferment sucrose, mannose, glucose, galactose, and lactose from the genomic research, which was confirmed by API 50 CH (bioMérieux, Marcy l'Etoile, France).
29055533	3	52	theme	possible	305:312	arg1	link					314:317	the possible link	301:317	the possible link between the genotype and the phenotype regarding EPS	301:370	To understand the possible link between the genotype and the phenotype regarding EPS, the complete genome of S. thermophilus KLDS SM was sequenced and investigated in silico for genes related to carbohydrate fermentation, nucleotide sugars synthesis, and EPS gene cluster.
29055533	7	53	theme	chemical	1297:1304	arg1	conformation					1306:1317	the chemical conformation	1293:1317	the chemical conformation of EPS	1293:1324	Our findings provide a better understanding of the link between the genetic elements and the chemical conformation of EPS and a theoretical basis for producing tailor-made EPS through genetic and metabolic engineering approaches.
29055533	4	54	theme	Marcy	740:744	arg1	Etoile					748:753	Marcy l'Etoile	740:753	Marcy l'Etoile	740:753	We found that S. thermophilus KLDS SM is able to ferment sucrose, mannose, glucose, galactose, and lactose from the genomic research, which was confirmed by API 50 CH (bioMérieux, Marcy l'Etoile, France).
29055533	4	54	theme	Marcy	740:744	arg1	bioMérieux					728:737	bioMérieux	728:737	bioMérieux	728:737	We found that S. thermophilus KLDS SM is able to ferment sucrose, mannose, glucose, galactose, and lactose from the genomic research, which was confirmed by API 50 CH (bioMérieux, Marcy l'Etoile, France).
29055533	6	55	theme	determination	1157:1169	arg1	protein					1171:1177	chain length determination protein	1144:1177	chain length determination protein	1144:1177	Furthermore, differences in the molecular mass of EPS from S. thermophilus KLDS SM cultivated under different carbon sources were correlated with the transcription levels of the genes encoding chain length determination protein and glycosyltransferase.
29055533	7	56	theme	theoretical	1332:1342	arg1	basis					1344:1348	a theoretical basis	1330:1348	a theoretical basis for producing tailor-made EPS through genetic and metabolic engineering approaches	1330:1431	Our findings provide a better understanding of the link between the genetic elements and the chemical conformation of EPS and a theoretical basis for producing tailor-made EPS through genetic and metabolic engineering approaches.
29055533	3	57	theme	EPS	542:544	arg1	cluster					551:557	EPS gene cluster	542:557	EPS gene cluster	542:557	To understand the possible link between the genotype and the phenotype regarding EPS, the complete genome of S. thermophilus KLDS SM was sequenced and investigated in silico for genes related to carbohydrate fermentation, nucleotide sugars synthesis, and EPS gene cluster.
29055533	4	58	dep	able	601:604	arg1	mannose					626:632	mannose	626:632	mannose	626:632	We found that S. thermophilus KLDS SM is able to ferment sucrose, mannose, glucose, galactose, and lactose from the genomic research, which was confirmed by API 50 CH (bioMérieux, Marcy l'Etoile, France).
29055533	4	58	dep	able	601:604	arg1	galactose					644:652	galactose	644:652	galactose	644:652	We found that S. thermophilus KLDS SM is able to ferment sucrose, mannose, glucose, galactose, and lactose from the genomic research, which was confirmed by API 50 CH (bioMérieux, Marcy l'Etoile, France).
29055533	4	58	dep	able	601:604	arg1	lactose					659:665	lactose	659:665	lactose	659:665	We found that S. thermophilus KLDS SM is able to ferment sucrose, mannose, glucose, galactose, and lactose from the genomic research, which was confirmed by API 50 CH (bioMérieux, Marcy l'Etoile, France).
29055533	4	58	dep	able	601:604	arg1	glucose					635:641	glucose	635:641	glucose	635:641	We found that S. thermophilus KLDS SM is able to ferment sucrose, mannose, glucose, galactose, and lactose from the genomic research, which was confirmed by API 50 CH (bioMérieux, Marcy l'Etoile, France).
29055533	6	59	theme	genes	1129:1133	arg1	levels					1115:1120	the transcription levels	1097:1120	the transcription levels of the genes encoding chain length determination protein and glycosyltransferase	1097:1201	Furthermore, differences in the molecular mass of EPS from S. thermophilus KLDS SM cultivated under different carbon sources were correlated with the transcription levels of the genes encoding chain length determination protein and glycosyltransferase.
29055533	1	60	theme	important	156:164	arg1	roles					166:170	important roles	156:170	important roles	156:170	Streptococcus thermophilus plays important roles in the dairy industry.
29055533	4	61	from	research	684:691	arg1	mannose					626:632	mannose	626:632	mannose	626:632	We found that S. thermophilus KLDS SM is able to ferment sucrose, mannose, glucose, galactose, and lactose from the genomic research, which was confirmed by API 50 CH (bioMérieux, Marcy l'Etoile, France).
29055533	4	61	from	research	684:691	arg1	galactose					644:652	galactose	644:652	galactose	644:652	We found that S. thermophilus KLDS SM is able to ferment sucrose, mannose, glucose, galactose, and lactose from the genomic research, which was confirmed by API 50 CH (bioMérieux, Marcy l'Etoile, France).
29055533	4	61	from	research	684:691	arg1	lactose					659:665	lactose	659:665	lactose	659:665	We found that S. thermophilus KLDS SM is able to ferment sucrose, mannose, glucose, galactose, and lactose from the genomic research, which was confirmed by API 50 CH (bioMérieux, Marcy l'Etoile, France).
29055533	4	61	from	research	684:691	arg1	glucose					635:641	glucose	635:641	glucose	635:641	We found that S. thermophilus KLDS SM is able to ferment sucrose, mannose, glucose, galactose, and lactose from the genomic research, which was confirmed by API 50 CH (bioMérieux, Marcy l'Etoile, France).
29055533	2	62	theme	KLDS	222:225	arg1	SM					227:228	Streptococcus thermophilus KLDS SM	195:228	Streptococcus thermophilus KLDS SM	195:228	Streptococcus thermophilus KLDS SM could produce a high amount of exopolysaccharides (EPS).
29055533	0	63	dep	communication	6:18	arg1	analyses					44:51	Genomic and phenotypic analyses	21:51	Short communication: Genomic and phenotypic analyses of exopolysaccharides produced by Streptococcus thermophilus	0:112	Short communication: Genomic and phenotypic analyses of exopolysaccharides produced by Streptococcus thermophilus KLDS SM.
29055533	5	64	theme	cluster	815:821	arg1	analysis					777:784	The genetic analysis	765:784	The genetic analysis of nucleotide sugars and EPS cluster	765:821	The genetic analysis of nucleotide sugars and EPS cluster revealed that the EPS produced by this strain are composed of galactose and glucose, in accordance with the biochemical result.
29055533	0	65	theme	exopolysaccharides	56:73	arg1	analyses					44:51	Genomic and phenotypic analyses	21:51	Short communication: Genomic and phenotypic analyses of exopolysaccharides produced by Streptococcus thermophilus	0:112	Short communication: Genomic and phenotypic analyses of exopolysaccharides produced by Streptococcus thermophilus KLDS SM.
29055533	2	66	theme	exopolysaccharides	261:278	arg1	amount					251:256	a high amount	244:256	a high amount of exopolysaccharides (EPS)	244:284	Streptococcus thermophilus KLDS SM could produce a high amount of exopolysaccharides (EPS).
29055533	2	66	theme	exopolysaccharides	261:278	arg1	EPS					281:283	EPS	281:283	EPS	281:283	Streptococcus thermophilus KLDS SM could produce a high amount of exopolysaccharides (EPS).
29055533	2	66	theme	exopolysaccharides	261:278	arg1	exopolysaccharides					261:278	exopolysaccharides	261:278	exopolysaccharides (EPS)	261:284	Streptococcus thermophilus KLDS SM could produce a high amount of exopolysaccharides (EPS).
25498656	3	0	theme	stable	625:630	arg1	Se/s-LNT					647:654	a stable nano-composite Se/s-LNT	623:654	a stable nano-composite Se/s-LNT	623:654	Transmission electron microscopy (TEM), energy dispersive X-ray (EDX) spectra, UV/vis, X-ray diffraction (XRD) and dynamic light scattering (DLS) showed that s-LNT coated SeNPs to form a stable nano-composite Se/s-LNT, leading to good dispersion of SeNPs.
25498656	1	1	theme	selenium	158:165	arg1	SeNPs					182:186	SeNPs	182:186	SeNPs	182:186	We report on a green procedure for the stabilization of selenium nanoparticles (SeNPs) by a naturally occurring β-glucan with triple helical conformation known as Lentinan (t-LNT) in water after denaturing into single chains (s-LNT) at 140 °C.
25498656	1	1	theme	selenium	158:165	arg1	nanoparticles					167:179	selenium nanoparticles	158:179	selenium nanoparticles (SeNPs)	158:187	We report on a green procedure for the stabilization of selenium nanoparticles (SeNPs) by a naturally occurring β-glucan with triple helical conformation known as Lentinan (t-LNT) in water after denaturing into single chains (s-LNT) at 140 °C.
25498656	0	2	theme	activity	92:99	arg1	enhancement					63:73	enhancement	63:73	enhancement of the antitumor activity	63:99	Construction of selenium nanoparticles/β-glucan composites for enhancement of the antitumor activity.
25498656	3	3	theme	energy	478:483	arg1	spectra					508:514	energy dispersive X-ray (EDX) spectra	478:514	energy dispersive X-ray (EDX) spectra	478:514	Transmission electron microscopy (TEM), energy dispersive X-ray (EDX) spectra, UV/vis, X-ray diffraction (XRD) and dynamic light scattering (DLS) showed that s-LNT coated SeNPs to form a stable nano-composite Se/s-LNT, leading to good dispersion of SeNPs.
25498656	6	4	with	SeNPs	1068:1072	arg1	distribution					1105:1116	particle size distribution	1091:1116	more evenly particle size distribution	1079:1116	The size effect of SeNPs on anti-tumor activity was revealed that the SeNPs with more evenly particle size distribution show the higher anticancer activity.
25498656	6	5	from	effect	1007:1012	arg1	activity					1037:1044	anti-tumor activity	1026:1044	anti-tumor activity	1026:1044	The size effect of SeNPs on anti-tumor activity was revealed that the SeNPs with more evenly particle size distribution show the higher anticancer activity.
25498656	6	6	theme	higher	1127:1132	arg1	activity					1145:1152	the higher anticancer activity	1123:1152	the higher anticancer activity	1123:1152	The size effect of SeNPs on anti-tumor activity was revealed that the SeNPs with more evenly particle size distribution show the higher anticancer activity.
25498656	6	7	theme	particle	1091:1098	arg1	distribution					1105:1116	particle size distribution	1091:1116	more evenly particle size distribution	1079:1116	The size effect of SeNPs on anti-tumor activity was revealed that the SeNPs with more evenly particle size distribution show the higher anticancer activity.
25498656	5	8	theme	size	846:849	arg1	distribution					851:862	Different size distribution	836:862	Different size distribution of SeNPs	836:871	Different size distribution of SeNPs was prepared by simply controlling the concentrations of selenite sodium and the corresponding reducing agent ascorbic acid.
25498656	3	9	theme	nano-composite	632:645	arg1	Se/s-LNT					647:654	a stable nano-composite Se/s-LNT	623:654	a stable nano-composite Se/s-LNT	623:654	Transmission electron microscopy (TEM), energy dispersive X-ray (EDX) spectra, UV/vis, X-ray diffraction (XRD) and dynamic light scattering (DLS) showed that s-LNT coated SeNPs to form a stable nano-composite Se/s-LNT, leading to good dispersion of SeNPs.
25498656	3	10	theme	EDX	503:505	arg1	spectra					508:514	energy dispersive X-ray (EDX) spectra	478:514	energy dispersive X-ray (EDX) spectra	478:514	Transmission electron microscopy (TEM), energy dispersive X-ray (EDX) spectra, UV/vis, X-ray diffraction (XRD) and dynamic light scattering (DLS) showed that s-LNT coated SeNPs to form a stable nano-composite Se/s-LNT, leading to good dispersion of SeNPs.
25498656	6	11	theme	anti-tumor	1026:1035	arg1	activity					1037:1044	anti-tumor activity	1026:1044	anti-tumor activity	1026:1044	The size effect of SeNPs on anti-tumor activity was revealed that the SeNPs with more evenly particle size distribution show the higher anticancer activity.
25498656	6	12	theme	SeNPs	1017:1021	arg1	effect					1007:1012	The size effect	998:1012	The size effect of SeNPs on anti-tumor activity	998:1044	The size effect of SeNPs on anti-tumor activity was revealed that the SeNPs with more evenly particle size distribution show the higher anticancer activity.
25498656	2	13	theme	Se-O-H	418:423	arg1	interaction					425:435	Se-O-H interaction	418:435	Se-O-H interaction	418:435	The results demonstrated that the s-LNT can interact with SeNPs through Se-O-H interaction.
25498656	6	14	theme	size	1100:1103	arg1	distribution					1105:1116	particle size distribution	1091:1116	more evenly particle size distribution	1079:1116	The size effect of SeNPs on anti-tumor activity was revealed that the SeNPs with more evenly particle size distribution show the higher anticancer activity.
25498656	3	15	theme	X-ray	525:529	arg1	XRD					544:546	XRD	544:546	XRD	544:546	Transmission electron microscopy (TEM), energy dispersive X-ray (EDX) spectra, UV/vis, X-ray diffraction (XRD) and dynamic light scattering (DLS) showed that s-LNT coated SeNPs to form a stable nano-composite Se/s-LNT, leading to good dispersion of SeNPs.
25498656	3	15	theme	X-ray	525:529	arg1	diffraction					531:541	X-ray diffraction	525:541	X-ray diffraction (XRD)	525:547	Transmission electron microscopy (TEM), energy dispersive X-ray (EDX) spectra, UV/vis, X-ray diffraction (XRD) and dynamic light scattering (DLS) showed that s-LNT coated SeNPs to form a stable nano-composite Se/s-LNT, leading to good dispersion of SeNPs.
25498656	5	16	theme	Different	836:844	arg1	distribution					851:862	Different size distribution	836:862	Different size distribution of SeNPs	836:871	Different size distribution of SeNPs was prepared by simply controlling the concentrations of selenite sodium and the corresponding reducing agent ascorbic acid.
25498656	1	17	from	Lentinan	265:272	arg1	water					285:289	water	285:289	water	285:289	We report on a green procedure for the stabilization of selenium nanoparticles (SeNPs) by a naturally occurring β-glucan with triple helical conformation known as Lentinan (t-LNT) in water after denaturing into single chains (s-LNT) at 140 °C.
25498656	5	18	theme	acid	992:995	arg1	concentrations					912:925	the concentrations	908:925	the concentrations of selenite sodium and the corresponding reducing agent ascorbic acid	908:995	Different size distribution of SeNPs was prepared by simply controlling the concentrations of selenite sodium and the corresponding reducing agent ascorbic acid.
25498656	5	19	theme	corresponding	954:966	arg1	acid					992:995	the corresponding reducing agent ascorbic acid	950:995	the corresponding reducing agent ascorbic acid	950:995	Different size distribution of SeNPs was prepared by simply controlling the concentrations of selenite sodium and the corresponding reducing agent ascorbic acid.
25498656	3	20	theme	Transmission	438:449	arg1	TEM					472:474	TEM	472:474	TEM	472:474	Transmission electron microscopy (TEM), energy dispersive X-ray (EDX) spectra, UV/vis, X-ray diffraction (XRD) and dynamic light scattering (DLS) showed that s-LNT coated SeNPs to form a stable nano-composite Se/s-LNT, leading to good dispersion of SeNPs.
25498656	3	20	theme	Transmission	438:449	arg1	microscopy					460:469	Transmission electron microscopy	438:469	Transmission electron microscopy (TEM)	438:475	Transmission electron microscopy (TEM), energy dispersive X-ray (EDX) spectra, UV/vis, X-ray diffraction (XRD) and dynamic light scattering (DLS) showed that s-LNT coated SeNPs to form a stable nano-composite Se/s-LNT, leading to good dispersion of SeNPs.
25498656	0	21	theme	selenium	16:23	arg1	Construction					0:11	Construction	0:11	Construction of selenium	0:23	Construction of selenium nanoparticles/β-glucan composites for enhancement of the antitumor activity.
25498656	1	22	with	β-glucan	214:221	arg1	conformation					243:254	triple helical conformation	228:254	triple helical conformation known as Lentinan (t-LNT) in water after denaturing into single chains (s-LNT) at 140 °C	228:343	We report on a green procedure for the stabilization of selenium nanoparticles (SeNPs) by a naturally occurring β-glucan with triple helical conformation known as Lentinan (t-LNT) in water after denaturing into single chains (s-LNT) at 140 °C.
25498656	1	23	theme	nanoparticles	167:179	arg1	stabilization					141:153	the stabilization	137:153	the stabilization of selenium nanoparticles (SeNPs) by a naturally occurring β-glucan with triple helical conformation known as Lentinan (t-LNT) in water after denaturing into single chains (s-LNT) at 140 °C	137:343	We report on a green procedure for the stabilization of selenium nanoparticles (SeNPs) by a naturally occurring β-glucan with triple helical conformation known as Lentinan (t-LNT) in water after denaturing into single chains (s-LNT) at 140 °C.
25498656	3	24	theme	electron	451:458	arg1	TEM					472:474	TEM	472:474	TEM	472:474	Transmission electron microscopy (TEM), energy dispersive X-ray (EDX) spectra, UV/vis, X-ray diffraction (XRD) and dynamic light scattering (DLS) showed that s-LNT coated SeNPs to form a stable nano-composite Se/s-LNT, leading to good dispersion of SeNPs.
25498656	3	24	theme	electron	451:458	arg1	microscopy					460:469	Transmission electron microscopy	438:469	Transmission electron microscopy (TEM)	438:475	Transmission electron microscopy (TEM), energy dispersive X-ray (EDX) spectra, UV/vis, X-ray diffraction (XRD) and dynamic light scattering (DLS) showed that s-LNT coated SeNPs to form a stable nano-composite Se/s-LNT, leading to good dispersion of SeNPs.
25498656	3	25	theme	good	668:671	arg1	dispersion					673:682	good dispersion	668:682	good dispersion of SeNPs	668:691	Transmission electron microscopy (TEM), energy dispersive X-ray (EDX) spectra, UV/vis, X-ray diffraction (XRD) and dynamic light scattering (DLS) showed that s-LNT coated SeNPs to form a stable nano-composite Se/s-LNT, leading to good dispersion of SeNPs.
25498656	4	26	theme	Se/s-LNT	722:729	arg1	composite					731:739	the as-prepared Se/s-LNT composite	706:739	the as-prepared Se/s-LNT composite in the solution	706:755	Especially, the as-prepared Se/s-LNT composite in the solution could remain homogeneous and translucent for 30 days without any precipitates.
25498656	6	27	theme	anticancer	1134:1143	arg1	activity					1145:1152	the higher anticancer activity	1123:1152	the higher anticancer activity	1123:1152	The size effect of SeNPs on anti-tumor activity was revealed that the SeNPs with more evenly particle size distribution show the higher anticancer activity.
25498656	1	28	theme	occurring	204:212	arg1	β-glucan					214:221	a naturally occurring β-glucan	192:221	a naturally occurring β-glucan with triple helical conformation known as Lentinan (t-LNT) in water after denaturing into single chains (s-LNT) at 140 °C	192:343	We report on a green procedure for the stabilization of selenium nanoparticles (SeNPs) by a naturally occurring β-glucan with triple helical conformation known as Lentinan (t-LNT) in water after denaturing into single chains (s-LNT) at 140 °C.
25498656	5	29	theme	reducing	968:975	arg1	acid					992:995	the corresponding reducing agent ascorbic acid	950:995	the corresponding reducing agent ascorbic acid	950:995	Different size distribution of SeNPs was prepared by simply controlling the concentrations of selenite sodium and the corresponding reducing agent ascorbic acid.
25498656	5	30	theme	agent	977:981	arg1	acid					992:995	the corresponding reducing agent ascorbic acid	950:995	the corresponding reducing agent ascorbic acid	950:995	Different size distribution of SeNPs was prepared by simply controlling the concentrations of selenite sodium and the corresponding reducing agent ascorbic acid.
25498656	1	31	theme	green	117:121	arg1	procedure					123:131	a green procedure	115:131	a green procedure for the stabilization of selenium nanoparticles (SeNPs) by a naturally occurring β-glucan with triple helical conformation known as Lentinan (t-LNT) in water after denaturing into single chains (s-LNT) at 140 °C	115:343	We report on a green procedure for the stabilization of selenium nanoparticles (SeNPs) by a naturally occurring β-glucan with triple helical conformation known as Lentinan (t-LNT) in water after denaturing into single chains (s-LNT) at 140 °C.
25498656	3	32	theme	SeNPs	687:691	arg1	dispersion					673:682	good dispersion	668:682	good dispersion of SeNPs	668:691	Transmission electron microscopy (TEM), energy dispersive X-ray (EDX) spectra, UV/vis, X-ray diffraction (XRD) and dynamic light scattering (DLS) showed that s-LNT coated SeNPs to form a stable nano-composite Se/s-LNT, leading to good dispersion of SeNPs.
25498656	3	33	theme	coated	602:607	arg1	SeNPs					609:613	s-LNT coated SeNPs	596:613	s-LNT coated SeNPs	596:613	Transmission electron microscopy (TEM), energy dispersive X-ray (EDX) spectra, UV/vis, X-ray diffraction (XRD) and dynamic light scattering (DLS) showed that s-LNT coated SeNPs to form a stable nano-composite Se/s-LNT, leading to good dispersion of SeNPs.
25498656	5	34	theme	ascorbic	983:990	arg1	acid					992:995	the corresponding reducing agent ascorbic acid	950:995	the corresponding reducing agent ascorbic acid	950:995	Different size distribution of SeNPs was prepared by simply controlling the concentrations of selenite sodium and the corresponding reducing agent ascorbic acid.
25498656	1	35	theme	single	313:318	arg1	s-LNT					328:332	s-LNT	328:332	s-LNT	328:332	We report on a green procedure for the stabilization of selenium nanoparticles (SeNPs) by a naturally occurring β-glucan with triple helical conformation known as Lentinan (t-LNT) in water after denaturing into single chains (s-LNT) at 140 °C.
25498656	1	35	theme	single	313:318	arg1	chains					320:325	single chains	313:325	single chains (s-LNT)	313:333	We report on a green procedure for the stabilization of selenium nanoparticles (SeNPs) by a naturally occurring β-glucan with triple helical conformation known as Lentinan (t-LNT) in water after denaturing into single chains (s-LNT) at 140 °C.
25498656	4	36	theme	as-prepared	710:720	arg1	composite					731:739	the as-prepared Se/s-LNT composite	706:739	the as-prepared Se/s-LNT composite in the solution	706:755	Especially, the as-prepared Se/s-LNT composite in the solution could remain homogeneous and translucent for 30 days without any precipitates.
25498656	3	37	theme	light	561:565	arg1	DLS					579:581	DLS	579:581	DLS	579:581	Transmission electron microscopy (TEM), energy dispersive X-ray (EDX) spectra, UV/vis, X-ray diffraction (XRD) and dynamic light scattering (DLS) showed that s-LNT coated SeNPs to form a stable nano-composite Se/s-LNT, leading to good dispersion of SeNPs.
25498656	3	37	theme	light	561:565	arg1	scattering					567:576	dynamic light scattering	553:576	dynamic light scattering (DLS)	553:582	Transmission electron microscopy (TEM), energy dispersive X-ray (EDX) spectra, UV/vis, X-ray diffraction (XRD) and dynamic light scattering (DLS) showed that s-LNT coated SeNPs to form a stable nano-composite Se/s-LNT, leading to good dispersion of SeNPs.
25498656	5	38	theme	sodium	939:944	arg1	concentrations					912:925	the concentrations	908:925	the concentrations of selenite sodium and the corresponding reducing agent ascorbic acid	908:995	Different size distribution of SeNPs was prepared by simply controlling the concentrations of selenite sodium and the corresponding reducing agent ascorbic acid.
25498656	4	39	from	composite	731:739	arg1	solution					748:755	the solution	744:755	the solution	744:755	Especially, the as-prepared Se/s-LNT composite in the solution could remain homogeneous and translucent for 30 days without any precipitates.
25498656	5	40	theme	SeNPs	867:871	arg1	distribution					851:862	Different size distribution	836:862	Different size distribution of SeNPs	836:871	Different size distribution of SeNPs was prepared by simply controlling the concentrations of selenite sodium and the corresponding reducing agent ascorbic acid.
25498656	1	41	theme	triple	228:233	arg1	conformation					243:254	triple helical conformation	228:254	triple helical conformation known as Lentinan (t-LNT) in water after denaturing into single chains (s-LNT) at 140 °C	228:343	We report on a green procedure for the stabilization of selenium nanoparticles (SeNPs) by a naturally occurring β-glucan with triple helical conformation known as Lentinan (t-LNT) in water after denaturing into single chains (s-LNT) at 140 °C.
25498656	0	42	theme	antitumor	82:90	arg1	activity					92:99	the antitumor activity	78:99	the antitumor activity	78:99	Construction of selenium nanoparticles/β-glucan composites for enhancement of the antitumor activity.
25498656	3	43	theme	s-LNT	596:600	arg1	SeNPs					609:613	s-LNT coated SeNPs	596:613	s-LNT coated SeNPs	596:613	Transmission electron microscopy (TEM), energy dispersive X-ray (EDX) spectra, UV/vis, X-ray diffraction (XRD) and dynamic light scattering (DLS) showed that s-LNT coated SeNPs to form a stable nano-composite Se/s-LNT, leading to good dispersion of SeNPs.
25498656	6	44	theme	size	1002:1005	arg1	effect					1007:1012	The size effect	998:1012	The size effect of SeNPs on anti-tumor activity	998:1044	The size effect of SeNPs on anti-tumor activity was revealed that the SeNPs with more evenly particle size distribution show the higher anticancer activity.
25498656	3	45	theme	dispersive	485:494	arg1	spectra					508:514	energy dispersive X-ray (EDX) spectra	478:514	energy dispersive X-ray (EDX) spectra	478:514	Transmission electron microscopy (TEM), energy dispersive X-ray (EDX) spectra, UV/vis, X-ray diffraction (XRD) and dynamic light scattering (DLS) showed that s-LNT coated SeNPs to form a stable nano-composite Se/s-LNT, leading to good dispersion of SeNPs.
25498656	1	46	theme	helical	235:241	arg1	conformation					243:254	triple helical conformation	228:254	triple helical conformation known as Lentinan (t-LNT) in water after denaturing into single chains (s-LNT) at 140 °C	228:343	We report on a green procedure for the stabilization of selenium nanoparticles (SeNPs) by a naturally occurring β-glucan with triple helical conformation known as Lentinan (t-LNT) in water after denaturing into single chains (s-LNT) at 140 °C.
25498656	5	47	theme	selenite	930:937	arg1	sodium					939:944	selenite sodium	930:944	selenite sodium	930:944	Different size distribution of SeNPs was prepared by simply controlling the concentrations of selenite sodium and the corresponding reducing agent ascorbic acid.
25498656	3	48	theme	dynamic	553:559	arg1	DLS					579:581	DLS	579:581	DLS	579:581	Transmission electron microscopy (TEM), energy dispersive X-ray (EDX) spectra, UV/vis, X-ray diffraction (XRD) and dynamic light scattering (DLS) showed that s-LNT coated SeNPs to form a stable nano-composite Se/s-LNT, leading to good dispersion of SeNPs.
25498656	3	48	theme	dynamic	553:559	arg1	scattering					567:576	dynamic light scattering	553:576	dynamic light scattering (DLS)	553:582	Transmission electron microscopy (TEM), energy dispersive X-ray (EDX) spectra, UV/vis, X-ray diffraction (XRD) and dynamic light scattering (DLS) showed that s-LNT coated SeNPs to form a stable nano-composite Se/s-LNT, leading to good dispersion of SeNPs.
25498656	3	49	theme	X-ray	496:500	arg1	spectra					508:514	energy dispersive X-ray (EDX) spectra	478:514	energy dispersive X-ray (EDX) spectra	478:514	Transmission electron microscopy (TEM), energy dispersive X-ray (EDX) spectra, UV/vis, X-ray diffraction (XRD) and dynamic light scattering (DLS) showed that s-LNT coated SeNPs to form a stable nano-composite Se/s-LNT, leading to good dispersion of SeNPs.
26788957	3	0	from	germination	498:508	arg1	events					450:455	gene transcription and alternative splicing events	406:455	gene transcription and alternative splicing events in four barley varieties at 24h and 48 h germination	406:508	We have investigated cell wall polysaccharide composition change, gene transcription and alternative splicing events in four barley varieties at 24h and 48 h germination.
26788957	3	0	from	germination	498:508	arg1	change					398:403	cell wall polysaccharide composition change	361:403	cell wall polysaccharide composition change	361:403	We have investigated cell wall polysaccharide composition change, gene transcription and alternative splicing events in four barley varieties at 24h and 48 h germination.
26788957	3	0	from	germination	498:508	arg1	varieties					472:480	four barley varieties	460:480	four barley varieties at 24h and 48 h germination	460:508	We have investigated cell wall polysaccharide composition change, gene transcription and alternative splicing events in four barley varieties at 24h and 48 h germination.
26788957	8	1	theme	cellulose	1110:1118	arg1	degradation					1095:1105	degradation	1095:1105	degradation	1095:1105	These genes coded enzymes catalyzing synthesis and degradation of cellulose, heteroxylan, (1,3)(1,4)-β-D-glucan and other cell wall polymers.
26788957	8	1	theme	cellulose	1110:1118	arg1	synthesis					1081:1089	synthesis	1081:1089	synthesis	1081:1089	These genes coded enzymes catalyzing synthesis and degradation of cellulose, heteroxylan, (1,3)(1,4)-β-D-glucan and other cell wall polymers.
26788957	6	2	theme	cell	854:857	arg1	modifications					864:876	cell wall modifications	854:876	cell wall modifications	854:876	Alternative splicing played very important roles in cell wall modifications.
26788957	10	3	theme	cellulase	1321:1329	arg1	synthase					1331:1338	primary wall cellulase synthase	1308:1338	primary wall cellulase synthase	1308:1338	Transcript levels of primary wall cellulase synthase, heteroxylan synthesizing and nucleotide sugar inter-conversion genes were very high in germinating embryos.
26788957	8	4	theme	heteroxylan	1121:1131	arg1	degradation					1095:1105	degradation	1095:1105	degradation	1095:1105	These genes coded enzymes catalyzing synthesis and degradation of cellulose, heteroxylan, (1,3)(1,4)-β-D-glucan and other cell wall polymers.
26788957	8	4	theme	heteroxylan	1121:1131	arg1	synthesis					1081:1089	synthesis	1081:1089	synthesis	1081:1089	These genes coded enzymes catalyzing synthesis and degradation of cellulose, heteroxylan, (1,3)(1,4)-β-D-glucan and other cell wall polymers.
26788957	8	5	theme	other	1160:1164	arg1	polymers					1176:1183	other cell wall polymers	1160:1183	other cell wall polymers	1160:1183	These genes coded enzymes catalyzing synthesis and degradation of cellulose, heteroxylan, (1,3)(1,4)-β-D-glucan and other cell wall polymers.
26788957	3	6	from	24h	485:487	arg1	events					450:455	gene transcription and alternative splicing events	406:455	gene transcription and alternative splicing events in four barley varieties at 24h and 48 h germination	406:508	We have investigated cell wall polysaccharide composition change, gene transcription and alternative splicing events in four barley varieties at 24h and 48 h germination.
26788957	3	6	from	24h	485:487	arg1	change					398:403	cell wall polysaccharide composition change	361:403	cell wall polysaccharide composition change	361:403	We have investigated cell wall polysaccharide composition change, gene transcription and alternative splicing events in four barley varieties at 24h and 48 h germination.
26788957	3	6	from	24h	485:487	arg1	varieties					472:480	four barley varieties	460:480	four barley varieties at 24h and 48 h germination	460:508	We have investigated cell wall polysaccharide composition change, gene transcription and alternative splicing events in four barley varieties at 24h and 48 h germination.
26788957	8	7	theme	wall	1171:1174	arg1	polymers					1176:1183	other cell wall polymers	1160:1183	other cell wall polymers	1160:1183	These genes coded enzymes catalyzing synthesis and degradation of cellulose, heteroxylan, (1,3)(1,4)-β-D-glucan and other cell wall polymers.
26788957	7	8	theme	alternative	1022:1032	arg1	splicing					1034:1041	alternative splicing	1022:1041	alternative splicing	1022:1041	At least 22 cell wall transcripts were detected to undergo either alternative 3' splicing, alternative 5' splicing or intron retention type of alternative splicing.
26788957	10	9	theme	synthesizing	1353:1364	arg1	heteroxylan					1341:1351	heteroxylan synthesizing	1341:1364	heteroxylan synthesizing	1341:1364	Transcript levels of primary wall cellulase synthase, heteroxylan synthesizing and nucleotide sugar inter-conversion genes were very high in germinating embryos.
26788957	2	10	theme	wall	297:300	arg1	modifications					302:314	cell wall modifications	292:314	cell wall modifications in germinating embryos	292:337	However, little is known on cell wall modifications in germinating embryos.
26788957	1	11	theme	important	184:192	arg1	membrane					228:235	membrane	228:235	membrane	228:235	Barley (Hordeum vulgare L.) seed germination initiates many important biological processes such as DNA, membrane and mitochondrial repairs.
26788957	1	11	theme	important	184:192	arg1	processes					205:213	many important biological processes	179:213	many important biological processes such as DNA, membrane and mitochondrial repairs	179:261	Barley (Hordeum vulgare L.) seed germination initiates many important biological processes such as DNA, membrane and mitochondrial repairs.
26788957	1	11	theme	important	184:192	arg1	DNA					223:225	DNA	223:225	DNA	223:225	Barley (Hordeum vulgare L.) seed germination initiates many important biological processes such as DNA, membrane and mitochondrial repairs.
26788957	1	11	theme	important	184:192	arg1	mitochondrial					241:253	mitochondrial	241:253	mitochondrial	241:253	Barley (Hordeum vulgare L.) seed germination initiates many important biological processes such as DNA, membrane and mitochondrial repairs.
26788957	7	12	theme	intron	997:1002	arg1	retention					1004:1012	intron retention	997:1012	intron retention	997:1012	At least 22 cell wall transcripts were detected to undergo either alternative 3' splicing, alternative 5' splicing or intron retention type of alternative splicing.
26788957	8	13	theme	-β-D-glucan	1144:1154	arg1	degradation					1095:1105	degradation	1095:1105	degradation	1095:1105	These genes coded enzymes catalyzing synthesis and degradation of cellulose, heteroxylan, (1,3)(1,4)-β-D-glucan and other cell wall polymers.
26788957	8	13	theme	-β-D-glucan	1144:1154	arg1	synthesis					1081:1089	synthesis	1081:1089	synthesis	1081:1089	These genes coded enzymes catalyzing synthesis and degradation of cellulose, heteroxylan, (1,3)(1,4)-β-D-glucan and other cell wall polymers.
26788957	12	14	theme	many	1541:1544	arg1	genes					1556:1560	many cell wall genes	1541:1560	many cell wall genes	1541:1560	Expression patterns of many cell wall genes coincided with changes in polysaccharide composition.
26788957	0	15	from	transcription	54:66	arg1	embryos					115:121	germinating barley embryos	96:121	germinating barley embryos	96:121	Changes in cell wall polysaccharide composition, gene transcription and alternative splicing in germinating barley embryos.
26788957	1	16	theme	Barley	124:129	arg1	germination					157:167	Barley (Hordeum vulgare L.) seed germination	124:167	Barley (Hordeum vulgare L.) seed germination	124:167	Barley (Hordeum vulgare L.) seed germination initiates many important biological processes such as DNA, membrane and mitochondrial repairs.
26788957	12	17	theme	wall	1551:1554	arg1	genes					1556:1560	many cell wall genes	1541:1560	many cell wall genes	1541:1560	Expression patterns of many cell wall genes coincided with changes in polysaccharide composition.
26788957	1	18	theme	Hordeum	132:138	arg1	vulgare					140:146	Hordeum vulgare	132:146	Hordeum vulgare	132:146	Barley (Hordeum vulgare L.) seed germination initiates many important biological processes such as DNA, membrane and mitochondrial repairs.
26788957	10	19	from	embryos	1440:1446	arg1	high					1420:1423	high	1420:1423	high	1420:1423	Transcript levels of primary wall cellulase synthase, heteroxylan synthesizing and nucleotide sugar inter-conversion genes were very high in germinating embryos.
26788957	9	20	theme	transcriptional	1199:1213	arg1	regulation					1215:1224	transcriptional regulation	1199:1224	transcriptional regulation	1199:1224	Furthermore, transcriptional regulation also played very important roles in cell wall modifications.
26788957	5	21	theme	type	759:762	arg1	levels					749:754	the levels	745:754	the levels of type I arabinogalactans and heteromannans	745:799	There were also significant changes in the levels of type I arabinogalactans and heteromannans.
26788957	0	22	theme	alternative	72:82	arg1	composition					36:46	cell wall polysaccharide composition	11:46	cell wall polysaccharide composition	11:46	Changes in cell wall polysaccharide composition, gene transcription and alternative splicing in germinating barley embryos.
26788957	0	22	theme	alternative	72:82	arg1	splicing					84:91	alternative splicing	72:91	alternative splicing	72:91	Changes in cell wall polysaccharide composition, gene transcription and alternative splicing in germinating barley embryos.
26788957	1	23	theme	seed	152:155	arg1	germination					157:167	Barley (Hordeum vulgare L.) seed germination	124:167	Barley (Hordeum vulgare L.) seed germination	124:167	Barley (Hordeum vulgare L.) seed germination initiates many important biological processes such as DNA, membrane and mitochondrial repairs.
26788957	2	24	from	modifications	302:314	arg1	embryos					331:337	germinating embryos	319:337	germinating embryos	319:337	However, little is known on cell wall modifications in germinating embryos.
26788957	0	25	from	Changes	0:6	arg1	composition					36:46	cell wall polysaccharide composition	11:46	cell wall polysaccharide composition	11:46	Changes in cell wall polysaccharide composition, gene transcription and alternative splicing in germinating barley embryos.
26788957	0	25	from	Changes	0:6	arg1	transcription					54:66	gene transcription	49:66	gene transcription	49:66	Changes in cell wall polysaccharide composition, gene transcription and alternative splicing in germinating barley embryos.
26788957	0	25	from	Changes	0:6	arg1	splicing					84:91	alternative splicing	72:91	alternative splicing	72:91	Changes in cell wall polysaccharide composition, gene transcription and alternative splicing in germinating barley embryos.
26788957	10	26	theme	inter-conversion	1387:1402	arg1	genes					1404:1408	primary wall cellulase synthase, heteroxylan synthesizing and nucleotide sugar inter-conversion genes	1308:1408	primary wall cellulase synthase, heteroxylan synthesizing and nucleotide sugar inter-conversion genes	1308:1408	Transcript levels of primary wall cellulase synthase, heteroxylan synthesizing and nucleotide sugar inter-conversion genes were very high in germinating embryos.
26788957	11	27	theme	cell	1461:1464	arg1	genes					1471:1475	At least 50 cell wall genes	1449:1475	At least 50 cell wall genes	1449:1475	At least 50 cell wall genes changed transcript levels significantly.
26788957	7	28	theme	wall	896:899	arg1	transcripts					901:911	At least 22 cell wall transcripts	879:911	At least 22 cell wall transcripts	879:911	At least 22 cell wall transcripts were detected to undergo either alternative 3' splicing, alternative 5' splicing or intron retention type of alternative splicing.
26788957	10	29	theme	germinating	1428:1438	arg1	embryos					1440:1446	germinating embryos	1428:1446	germinating embryos	1428:1446	Transcript levels of primary wall cellulase synthase, heteroxylan synthesizing and nucleotide sugar inter-conversion genes were very high in germinating embryos.
26788957	0	30	theme	barley	108:113	arg1	embryos					115:121	germinating barley embryos	96:121	germinating barley embryos	96:121	Changes in cell wall polysaccharide composition, gene transcription and alternative splicing in germinating barley embryos.
26788957	3	31	theme	composition	386:396	arg1	events					450:455	gene transcription and alternative splicing events	406:455	gene transcription and alternative splicing events in four barley varieties at 24h and 48 h germination	406:508	We have investigated cell wall polysaccharide composition change, gene transcription and alternative splicing events in four barley varieties at 24h and 48 h germination.
26788957	3	31	theme	composition	386:396	arg1	change					398:403	cell wall polysaccharide composition change	361:403	cell wall polysaccharide composition change	361:403	We have investigated cell wall polysaccharide composition change, gene transcription and alternative splicing events in four barley varieties at 24h and 48 h germination.
26788957	3	32	theme	alternative	429:439	arg1	splicing					441:448	alternative splicing	429:448	alternative splicing	429:448	We have investigated cell wall polysaccharide composition change, gene transcription and alternative splicing events in four barley varieties at 24h and 48 h germination.
26788957	9	33	theme	cell	1262:1265	arg1	modifications					1272:1284	cell wall modifications	1262:1284	cell wall modifications	1262:1284	Furthermore, transcriptional regulation also played very important roles in cell wall modifications.
26788957	3	34	theme	wall	366:369	arg1	events					450:455	gene transcription and alternative splicing events	406:455	gene transcription and alternative splicing events in four barley varieties at 24h and 48 h germination	406:508	We have investigated cell wall polysaccharide composition change, gene transcription and alternative splicing events in four barley varieties at 24h and 48 h germination.
26788957	3	34	theme	wall	366:369	arg1	change					398:403	cell wall polysaccharide composition change	361:403	cell wall polysaccharide composition change	361:403	We have investigated cell wall polysaccharide composition change, gene transcription and alternative splicing events in four barley varieties at 24h and 48 h germination.
26788957	8	35	dep	-β-D-glucan	1144:1154	arg1	1,4					1140:1142	1,4	1140:1142	1,4	1140:1142	These genes coded enzymes catalyzing synthesis and degradation of cellulose, heteroxylan, (1,3)(1,4)-β-D-glucan and other cell wall polymers.
26788957	11	36	theme	transcript	1485:1494	arg1	levels					1496:1501	transcript levels	1485:1501	transcript levels	1485:1501	At least 50 cell wall genes changed transcript levels significantly.
26788957	1	37	dep	vulgare	140:146	arg1	L.					148:149	L.	148:149	L.	148:149	Barley (Hordeum vulgare L.) seed germination initiates many important biological processes such as DNA, membrane and mitochondrial repairs.
26788957	0	38	theme	cell	11:14	arg1	composition					36:46	cell wall polysaccharide composition	11:46	cell wall polysaccharide composition	11:46	Changes in cell wall polysaccharide composition, gene transcription and alternative splicing in germinating barley embryos.
26788957	0	38	theme	cell	11:14	arg1	transcription					54:66	gene transcription	49:66	gene transcription	49:66	Changes in cell wall polysaccharide composition, gene transcription and alternative splicing in germinating barley embryos.
26788957	0	38	theme	cell	11:14	arg1	splicing					84:91	alternative splicing	72:91	alternative splicing	72:91	Changes in cell wall polysaccharide composition, gene transcription and alternative splicing in germinating barley embryos.
26788957	12	39	from	changes	1577:1583	arg1	composition					1603:1613	polysaccharide composition	1588:1613	polysaccharide composition	1588:1613	Expression patterns of many cell wall genes coincided with changes in polysaccharide composition.
26788957	3	40	theme	transcription	411:423	arg1	events					450:455	gene transcription and alternative splicing events	406:455	gene transcription and alternative splicing events in four barley varieties at 24h and 48 h germination	406:508	We have investigated cell wall polysaccharide composition change, gene transcription and alternative splicing events in four barley varieties at 24h and 48 h germination.
26788957	3	40	theme	transcription	411:423	arg1	change					398:403	cell wall polysaccharide composition change	361:403	cell wall polysaccharide composition change	361:403	We have investigated cell wall polysaccharide composition change, gene transcription and alternative splicing events in four barley varieties at 24h and 48 h germination.
26788957	0	41	theme	polysaccharide	21:34	arg1	composition					36:46	cell wall polysaccharide composition	11:46	cell wall polysaccharide composition	11:46	Changes in cell wall polysaccharide composition, gene transcription and alternative splicing in germinating barley embryos.
26788957	0	41	theme	polysaccharide	21:34	arg1	transcription					54:66	gene transcription	49:66	gene transcription	49:66	Changes in cell wall polysaccharide composition, gene transcription and alternative splicing in germinating barley embryos.
26788957	0	41	theme	polysaccharide	21:34	arg1	splicing					84:91	alternative splicing	72:91	alternative splicing	72:91	Changes in cell wall polysaccharide composition, gene transcription and alternative splicing in germinating barley embryos.
26788957	5	42	theme	significant	722:732	arg1	changes					734:740	significant changes	722:740	significant changes in the levels of type I arabinogalactans and heteromannans	722:799	There were also significant changes in the levels of type I arabinogalactans and heteromannans.
26788957	6	43	theme	Alternative	802:812	arg1	splicing					814:821	Alternative splicing	802:821	Alternative splicing	802:821	Alternative splicing played very important roles in cell wall modifications.
26788957	1	44	dep	germination	157:167	arg1	vulgare					140:146	Hordeum vulgare	132:146	Hordeum vulgare	132:146	Barley (Hordeum vulgare L.) seed germination initiates many important biological processes such as DNA, membrane and mitochondrial repairs.
26788957	3	45	theme	48	493:494	arg1	h					496:496	h	496:496	h	496:496	We have investigated cell wall polysaccharide composition change, gene transcription and alternative splicing events in four barley varieties at 24h and 48 h germination.
26788957	4	46	from	components	521:530	arg1	embryos					554:560	germinating barley embryos	535:560	germinating barley embryos	535:560	Cell wall components in germinating barley embryos changed rapidly, with increases in cellulose and (1,3)(1,4)-β-D-glucan (20-100%) within 24h, but decreases in heteroxylan and arabinan (3-50%).
26788957	5	47	from	changes	734:740	arg1	levels					749:754	the levels	745:754	the levels of type I arabinogalactans and heteromannans	745:799	There were also significant changes in the levels of type I arabinogalactans and heteromannans.
26788957	4	48	theme	germinating	535:545	arg1	embryos					554:560	germinating barley embryos	535:560	germinating barley embryos	535:560	Cell wall components in germinating barley embryos changed rapidly, with increases in cellulose and (1,3)(1,4)-β-D-glucan (20-100%) within 24h, but decreases in heteroxylan and arabinan (3-50%).
26788957	10	49	theme	nucleotide	1370:1379	arg1	inter-conversion					1387:1402	nucleotide sugar inter-conversion	1370:1402	nucleotide sugar inter-conversion	1370:1402	Transcript levels of primary wall cellulase synthase, heteroxylan synthesizing and nucleotide sugar inter-conversion genes were very high in germinating embryos.
26788957	2	50	theme	germinating	319:329	arg1	embryos					331:337	germinating embryos	319:337	germinating embryos	319:337	However, little is known on cell wall modifications in germinating embryos.
26788957	4	51	from	decreases	659:667	arg1	arabinan					688:695	arabinan	688:695	arabinan	688:695	Cell wall components in germinating barley embryos changed rapidly, with increases in cellulose and (1,3)(1,4)-β-D-glucan (20-100%) within 24h, but decreases in heteroxylan and arabinan (3-50%).
26788957	4	51	from	decreases	659:667	arg1	heteroxylan					672:682	heteroxylan	672:682	heteroxylan	672:682	Cell wall components in germinating barley embryos changed rapidly, with increases in cellulose and (1,3)(1,4)-β-D-glucan (20-100%) within 24h, but decreases in heteroxylan and arabinan (3-50%).
26788957	10	52	theme	Transcript	1287:1296	arg1	levels					1298:1303	Transcript levels	1287:1303	Transcript levels of primary wall cellulase synthase, heteroxylan synthesizing and nucleotide sugar inter-conversion genes	1287:1408	Transcript levels of primary wall cellulase synthase, heteroxylan synthesizing and nucleotide sugar inter-conversion genes were very high in germinating embryos.
26788957	6	53	theme	wall	859:862	arg1	modifications					864:876	cell wall modifications	854:876	cell wall modifications	854:876	Alternative splicing played very important roles in cell wall modifications.
26788957	5	54	dep	type	759:762	arg1	heteromannans					787:799	heteromannans	787:799	heteromannans	787:799	There were also significant changes in the levels of type I arabinogalactans and heteromannans.
26788957	5	54	dep	type	759:762	arg1	arabinogalactans					766:781	arabinogalactans	766:781	arabinogalactans	766:781	There were also significant changes in the levels of type I arabinogalactans and heteromannans.
26788957	13	55	theme	transcriptional	1748:1762	arg1	levels					1789:1794	both transcriptional and post-transcriptional levels	1743:1794	both transcriptional and post-transcriptional levels	1743:1794	Our data showed that cell wall polysaccharide metabolism was very active in germinating barley embryos, which was regulated at both transcriptional and post-transcriptional levels.
26788957	10	56	theme	synthase	1331:1338	arg1	genes					1404:1408	primary wall cellulase synthase, heteroxylan synthesizing and nucleotide sugar inter-conversion genes	1308:1408	primary wall cellulase synthase, heteroxylan synthesizing and nucleotide sugar inter-conversion genes	1308:1408	Transcript levels of primary wall cellulase synthase, heteroxylan synthesizing and nucleotide sugar inter-conversion genes were very high in germinating embryos.
26788957	13	57	theme	post-transcriptional	1768:1787	arg1	levels					1789:1794	both transcriptional and post-transcriptional levels	1743:1794	both transcriptional and post-transcriptional levels	1743:1794	Our data showed that cell wall polysaccharide metabolism was very active in germinating barley embryos, which was regulated at both transcriptional and post-transcriptional levels.
26788957	7	58	theme	splicing	1034:1041	arg1	retention					1004:1012	intron retention	997:1012	intron retention	997:1012	At least 22 cell wall transcripts were detected to undergo either alternative 3' splicing, alternative 5' splicing or intron retention type of alternative splicing.
26788957	7	58	theme	splicing	1034:1041	arg1	splicing					985:992	alternative 5' splicing	970:992	alternative 5' splicing	970:992	At least 22 cell wall transcripts were detected to undergo either alternative 3' splicing, alternative 5' splicing or intron retention type of alternative splicing.
26788957	7	58	theme	splicing	1034:1041	arg1	splicing					960:967	either alternative 3' splicing	938:967	either alternative 3' splicing	938:967	At least 22 cell wall transcripts were detected to undergo either alternative 3' splicing, alternative 5' splicing or intron retention type of alternative splicing.
26788957	10	59	theme	wall	1316:1319	arg1	synthase					1331:1338	primary wall cellulase synthase	1308:1338	primary wall cellulase synthase	1308:1338	Transcript levels of primary wall cellulase synthase, heteroxylan synthesizing and nucleotide sugar inter-conversion genes were very high in germinating embryos.
26788957	6	60	theme	important	835:843	arg1	roles					845:849	very important roles	830:849	very important roles	830:849	Alternative splicing played very important roles in cell wall modifications.
26788957	1	61	theme	many	179:182	arg1	membrane					228:235	membrane	228:235	membrane	228:235	Barley (Hordeum vulgare L.) seed germination initiates many important biological processes such as DNA, membrane and mitochondrial repairs.
26788957	1	61	theme	many	179:182	arg1	processes					205:213	many important biological processes	179:213	many important biological processes such as DNA, membrane and mitochondrial repairs	179:261	Barley (Hordeum vulgare L.) seed germination initiates many important biological processes such as DNA, membrane and mitochondrial repairs.
26788957	1	61	theme	many	179:182	arg1	DNA					223:225	DNA	223:225	DNA	223:225	Barley (Hordeum vulgare L.) seed germination initiates many important biological processes such as DNA, membrane and mitochondrial repairs.
26788957	1	61	theme	many	179:182	arg1	mitochondrial					241:253	mitochondrial	241:253	mitochondrial	241:253	Barley (Hordeum vulgare L.) seed germination initiates many important biological processes such as DNA, membrane and mitochondrial repairs.
26788957	8	62	theme	cell	1166:1169	arg1	polymers					1176:1183	other cell wall polymers	1160:1183	other cell wall polymers	1160:1183	These genes coded enzymes catalyzing synthesis and degradation of cellulose, heteroxylan, (1,3)(1,4)-β-D-glucan and other cell wall polymers.
26788957	10	63	theme	heteroxylan	1341:1351	arg1	genes					1404:1408	primary wall cellulase synthase, heteroxylan synthesizing and nucleotide sugar inter-conversion genes	1308:1408	primary wall cellulase synthase, heteroxylan synthesizing and nucleotide sugar inter-conversion genes	1308:1408	Transcript levels of primary wall cellulase synthase, heteroxylan synthesizing and nucleotide sugar inter-conversion genes were very high in germinating embryos.
26788957	2	64	theme	cell	292:295	arg1	modifications					302:314	cell wall modifications	292:314	cell wall modifications in germinating embryos	292:337	However, little is known on cell wall modifications in germinating embryos.
26788957	1	65	theme	biological	194:203	arg1	membrane					228:235	membrane	228:235	membrane	228:235	Barley (Hordeum vulgare L.) seed germination initiates many important biological processes such as DNA, membrane and mitochondrial repairs.
26788957	1	65	theme	biological	194:203	arg1	processes					205:213	many important biological processes	179:213	many important biological processes such as DNA, membrane and mitochondrial repairs	179:261	Barley (Hordeum vulgare L.) seed germination initiates many important biological processes such as DNA, membrane and mitochondrial repairs.
26788957	1	65	theme	biological	194:203	arg1	DNA					223:225	DNA	223:225	DNA	223:225	Barley (Hordeum vulgare L.) seed germination initiates many important biological processes such as DNA, membrane and mitochondrial repairs.
26788957	1	65	theme	biological	194:203	arg1	mitochondrial					241:253	mitochondrial	241:253	mitochondrial	241:253	Barley (Hordeum vulgare L.) seed germination initiates many important biological processes such as DNA, membrane and mitochondrial repairs.
26788957	0	66	from	splicing	84:91	arg1	embryos					115:121	germinating barley embryos	96:121	germinating barley embryos	96:121	Changes in cell wall polysaccharide composition, gene transcription and alternative splicing in germinating barley embryos.
26788957	8	67	theme	polymers	1176:1183	arg1	degradation					1095:1105	degradation	1095:1105	degradation	1095:1105	These genes coded enzymes catalyzing synthesis and degradation of cellulose, heteroxylan, (1,3)(1,4)-β-D-glucan and other cell wall polymers.
26788957	8	67	theme	polymers	1176:1183	arg1	synthesis					1081:1089	synthesis	1081:1089	synthesis	1081:1089	These genes coded enzymes catalyzing synthesis and degradation of cellulose, heteroxylan, (1,3)(1,4)-β-D-glucan and other cell wall polymers.
26788957	13	68	theme	barley	1704:1709	arg1	embryos					1711:1717	barley embryos	1704:1717	barley embryos	1704:1717	Our data showed that cell wall polysaccharide metabolism was very active in germinating barley embryos, which was regulated at both transcriptional and post-transcriptional levels.
26788957	10	69	from	high	1420:1423	arg1	embryos					1440:1446	germinating embryos	1428:1446	germinating embryos	1428:1446	Transcript levels of primary wall cellulase synthase, heteroxylan synthesizing and nucleotide sugar inter-conversion genes were very high in germinating embryos.
26788957	4	70	theme	Cell	511:514	arg1	components					521:530	Cell wall components	511:530	Cell wall components in germinating barley embryos	511:560	Cell wall components in germinating barley embryos changed rapidly, with increases in cellulose and (1,3)(1,4)-β-D-glucan (20-100%) within 24h, but decreases in heteroxylan and arabinan (3-50%).
26788957	12	71	theme	genes	1556:1560	arg1	patterns					1529:1536	Expression patterns	1518:1536	Expression patterns of many cell wall genes	1518:1560	Expression patterns of many cell wall genes coincided with changes in polysaccharide composition.
26788957	7	72	theme	alternative	970:980	arg1	splicing					985:992	alternative 5' splicing	970:992	alternative 5' splicing	970:992	At least 22 cell wall transcripts were detected to undergo either alternative 3' splicing, alternative 5' splicing or intron retention type of alternative splicing.
26788957	12	73	theme	Expression	1518:1527	arg1	patterns					1529:1536	Expression patterns	1518:1536	Expression patterns of many cell wall genes	1518:1560	Expression patterns of many cell wall genes coincided with changes in polysaccharide composition.
26788957	1	74	dep	mitochondrial	241:253	arg1	repairs					255:261	repairs	255:261	repairs	255:261	Barley (Hordeum vulgare L.) seed germination initiates many important biological processes such as DNA, membrane and mitochondrial repairs.
26788957	7	75	theme	alternative	945:955	arg1	splicing					960:967	either alternative 3' splicing	938:967	either alternative 3' splicing	938:967	At least 22 cell wall transcripts were detected to undergo either alternative 3' splicing, alternative 5' splicing or intron retention type of alternative splicing.
26788957	11	76	theme	wall	1466:1469	arg1	genes					1471:1475	At least 50 cell wall genes	1449:1475	At least 50 cell wall genes	1449:1475	At least 50 cell wall genes changed transcript levels significantly.
26788957	4	77	theme	wall	516:519	arg1	components					521:530	Cell wall components	511:530	Cell wall components in germinating barley embryos	511:560	Cell wall components in germinating barley embryos changed rapidly, with increases in cellulose and (1,3)(1,4)-β-D-glucan (20-100%) within 24h, but decreases in heteroxylan and arabinan (3-50%).
26788957	10	78	theme	genes	1404:1408	arg1	levels					1298:1303	Transcript levels	1287:1303	Transcript levels of primary wall cellulase synthase, heteroxylan synthesizing and nucleotide sugar inter-conversion genes	1287:1408	Transcript levels of primary wall cellulase synthase, heteroxylan synthesizing and nucleotide sugar inter-conversion genes were very high in germinating embryos.
26788957	0	79	theme	germinating	96:106	arg1	embryos					115:121	germinating barley embryos	96:121	germinating barley embryos	96:121	Changes in cell wall polysaccharide composition, gene transcription and alternative splicing in germinating barley embryos.
26788957	3	80	theme	cell	361:364	arg1	events					450:455	gene transcription and alternative splicing events	406:455	gene transcription and alternative splicing events in four barley varieties at 24h and 48 h germination	406:508	We have investigated cell wall polysaccharide composition change, gene transcription and alternative splicing events in four barley varieties at 24h and 48 h germination.
26788957	3	80	theme	cell	361:364	arg1	change					398:403	cell wall polysaccharide composition change	361:403	cell wall polysaccharide composition change	361:403	We have investigated cell wall polysaccharide composition change, gene transcription and alternative splicing events in four barley varieties at 24h and 48 h germination.
26788957	10	81	theme	sugar	1381:1385	arg1	inter-conversion					1387:1402	nucleotide sugar inter-conversion	1370:1402	nucleotide sugar inter-conversion	1370:1402	Transcript levels of primary wall cellulase synthase, heteroxylan synthesizing and nucleotide sugar inter-conversion genes were very high in germinating embryos.
26788957	4	82	from	increases	584:592	arg1	cellulose					597:605	cellulose	597:605	cellulose	597:605	Cell wall components in germinating barley embryos changed rapidly, with increases in cellulose and (1,3)(1,4)-β-D-glucan (20-100%) within 24h, but decreases in heteroxylan and arabinan (3-50%).
26788957	4	82	from	increases	584:592	arg1	-β-D-glucan					621:631	-β-D-glucan	621:631	(1,3)(1,4)-β-D-glucan (20-100%) within 24h	611:652	Cell wall components in germinating barley embryos changed rapidly, with increases in cellulose and (1,3)(1,4)-β-D-glucan (20-100%) within 24h, but decreases in heteroxylan and arabinan (3-50%).
26788957	4	82	from	increases	584:592	arg1	1,3					612:614	1,3	612:614	1,3	612:614	Cell wall components in germinating barley embryos changed rapidly, with increases in cellulose and (1,3)(1,4)-β-D-glucan (20-100%) within 24h, but decreases in heteroxylan and arabinan (3-50%).
26788957	4	82	from	increases	584:592	arg1	%					640:640	20-100%	634:640	20-100%	634:640	Cell wall components in germinating barley embryos changed rapidly, with increases in cellulose and (1,3)(1,4)-β-D-glucan (20-100%) within 24h, but decreases in heteroxylan and arabinan (3-50%).
26788957	9	83	theme	important	1243:1251	arg1	roles					1253:1257	very important roles	1238:1257	very important roles	1238:1257	Furthermore, transcriptional regulation also played very important roles in cell wall modifications.
26788957	13	84	theme	polysaccharide	1647:1660	arg1	metabolism					1662:1671	cell wall polysaccharide metabolism	1637:1671	cell wall polysaccharide metabolism	1637:1671	Our data showed that cell wall polysaccharide metabolism was very active in germinating barley embryos, which was regulated at both transcriptional and post-transcriptional levels.
26788957	7	85	dep	splicing	960:967	arg1	type					1014:1017	type	1014:1017	type	1014:1017	At least 22 cell wall transcripts were detected to undergo either alternative 3' splicing, alternative 5' splicing or intron retention type of alternative splicing.
26788957	7	86	theme	cell	891:894	arg1	transcripts					901:911	At least 22 cell wall transcripts	879:911	At least 22 cell wall transcripts	879:911	At least 22 cell wall transcripts were detected to undergo either alternative 3' splicing, alternative 5' splicing or intron retention type of alternative splicing.
26788957	13	87	theme	wall	1642:1645	arg1	metabolism					1662:1671	cell wall polysaccharide metabolism	1637:1671	cell wall polysaccharide metabolism	1637:1671	Our data showed that cell wall polysaccharide metabolism was very active in germinating barley embryos, which was regulated at both transcriptional and post-transcriptional levels.
26788957	3	88	theme	splicing	441:448	arg1	events					450:455	gene transcription and alternative splicing events	406:455	gene transcription and alternative splicing events in four barley varieties at 24h and 48 h germination	406:508	We have investigated cell wall polysaccharide composition change, gene transcription and alternative splicing events in four barley varieties at 24h and 48 h germination.
26788957	3	88	theme	splicing	441:448	arg1	change					398:403	cell wall polysaccharide composition change	361:403	cell wall polysaccharide composition change	361:403	We have investigated cell wall polysaccharide composition change, gene transcription and alternative splicing events in four barley varieties at 24h and 48 h germination.
26788957	0	89	theme	wall	16:19	arg1	composition					36:46	cell wall polysaccharide composition	11:46	cell wall polysaccharide composition	11:46	Changes in cell wall polysaccharide composition, gene transcription and alternative splicing in germinating barley embryos.
26788957	0	89	theme	wall	16:19	arg1	transcription					54:66	gene transcription	49:66	gene transcription	49:66	Changes in cell wall polysaccharide composition, gene transcription and alternative splicing in germinating barley embryos.
26788957	0	89	theme	wall	16:19	arg1	splicing					84:91	alternative splicing	72:91	alternative splicing	72:91	Changes in cell wall polysaccharide composition, gene transcription and alternative splicing in germinating barley embryos.
26788957	9	90	theme	wall	1267:1270	arg1	modifications					1272:1284	cell wall modifications	1262:1284	cell wall modifications	1262:1284	Furthermore, transcriptional regulation also played very important roles in cell wall modifications.
26788957	12	91	theme	polysaccharide	1588:1601	arg1	composition					1603:1613	polysaccharide composition	1588:1613	polysaccharide composition	1588:1613	Expression patterns of many cell wall genes coincided with changes in polysaccharide composition.
26788957	3	92	theme	polysaccharide	371:384	arg1	events					450:455	gene transcription and alternative splicing events	406:455	gene transcription and alternative splicing events in four barley varieties at 24h and 48 h germination	406:508	We have investigated cell wall polysaccharide composition change, gene transcription and alternative splicing events in four barley varieties at 24h and 48 h germination.
26788957	3	92	theme	polysaccharide	371:384	arg1	change					398:403	cell wall polysaccharide composition change	361:403	cell wall polysaccharide composition change	361:403	We have investigated cell wall polysaccharide composition change, gene transcription and alternative splicing events in four barley varieties at 24h and 48 h germination.
26788957	4	93	dep	-β-D-glucan	621:631	arg1	1,4					617:619	1,4	617:619	1,4	617:619	Cell wall components in germinating barley embryos changed rapidly, with increases in cellulose and (1,3)(1,4)-β-D-glucan (20-100%) within 24h, but decreases in heteroxylan and arabinan (3-50%).
26788957	13	94	theme	cell	1637:1640	arg1	metabolism					1662:1671	cell wall polysaccharide metabolism	1637:1671	cell wall polysaccharide metabolism	1637:1671	Our data showed that cell wall polysaccharide metabolism was very active in germinating barley embryos, which was regulated at both transcriptional and post-transcriptional levels.
26788957	3	95	theme	barley	465:470	arg1	varieties					472:480	four barley varieties	460:480	four barley varieties at 24h and 48 h germination	460:508	We have investigated cell wall polysaccharide composition change, gene transcription and alternative splicing events in four barley varieties at 24h and 48 h germination.
26788957	0	96	theme	gene	49:52	arg1	composition					36:46	cell wall polysaccharide composition	11:46	cell wall polysaccharide composition	11:46	Changes in cell wall polysaccharide composition, gene transcription and alternative splicing in germinating barley embryos.
26788957	0	96	theme	gene	49:52	arg1	transcription					54:66	gene transcription	49:66	gene transcription	49:66	Changes in cell wall polysaccharide composition, gene transcription and alternative splicing in germinating barley embryos.
26788957	12	97	theme	cell	1546:1549	arg1	genes					1556:1560	many cell wall genes	1541:1560	many cell wall genes	1541:1560	Expression patterns of many cell wall genes coincided with changes in polysaccharide composition.
26788957	3	98	theme	gene	406:409	arg1	transcription					411:423	gene transcription	406:423	gene transcription	406:423	We have investigated cell wall polysaccharide composition change, gene transcription and alternative splicing events in four barley varieties at 24h and 48 h germination.
26788957	4	99	theme	barley	547:552	arg1	embryos					554:560	germinating barley embryos	535:560	germinating barley embryos	535:560	Cell wall components in germinating barley embryos changed rapidly, with increases in cellulose and (1,3)(1,4)-β-D-glucan (20-100%) within 24h, but decreases in heteroxylan and arabinan (3-50%).
26788957	3	100	theme	h	496:496	arg1	germination					498:508	48 h germination	493:508	48 h germination	493:508	We have investigated cell wall polysaccharide composition change, gene transcription and alternative splicing events in four barley varieties at 24h and 48 h germination.
26788957	10	101	theme	primary	1308:1314	arg1	synthase					1331:1338	primary wall cellulase synthase	1308:1338	primary wall cellulase synthase	1308:1338	Transcript levels of primary wall cellulase synthase, heteroxylan synthesizing and nucleotide sugar inter-conversion genes were very high in germinating embryos.
26788957	3	102	from	events	450:455	arg1	24h					485:487	24h	485:487	24h	485:487	We have investigated cell wall polysaccharide composition change, gene transcription and alternative splicing events in four barley varieties at 24h and 48 h germination.
26788957	3	102	from	events	450:455	arg1	varieties					472:480	four barley varieties	460:480	four barley varieties at 24h and 48 h germination	460:508	We have investigated cell wall polysaccharide composition change, gene transcription and alternative splicing events in four barley varieties at 24h and 48 h germination.
26788957	3	102	from	events	450:455	arg1	germination					498:508	48 h germination	493:508	48 h germination	493:508	We have investigated cell wall polysaccharide composition change, gene transcription and alternative splicing events in four barley varieties at 24h and 48 h germination.
27084870	0	0	theme	Intestinal	75:84	arg1	Heparins					86:93	Porcine Intestinal Heparins	67:93	Porcine Intestinal Heparins	67:93	Analysis of Heparins Derived From Bovine Tissues and Comparison to Porcine Intestinal Heparins.
27084870	9	1	theme	functional	1202:1211	arg1	activities					1213:1222	The in vitro functional activities	1189:1222	The in vitro functional activities of these heparins	1189:1240	The in vitro functional activities of these heparins have also been determined.
27084870	7	2	theme	bovine	889:894	arg1	heparins					896:903	bovine heparins	889:903	bovine heparins prepared from both intestines and lung	889:942	The current study examines bovine heparins prepared from both intestines and lung and compares these to porcine intestinal heparin.
27084870	0	3	theme	Porcine	67:73	arg1	Heparins					86:93	Porcine Intestinal Heparins	67:93	Porcine Intestinal Heparins	67:93	Analysis of Heparins Derived From Bovine Tissues and Comparison to Porcine Intestinal Heparins.
27084870	4	4	theme	Pharmaceutical	490:503	arg1	heparin					505:511	Pharmaceutical heparin	490:511	Pharmaceutical heparin currently used in the United States	490:547	Pharmaceutical heparin currently used in the United States is primarily extracted from porcine intestines.
27084870	8	5	theme	heparins	1029:1036	arg1	properties					1009:1018	The structural properties	994:1018	The structural properties of these heparins	994:1036	The structural properties of these heparins are examined using nuclear magnetic resonance, gel permeation chromatography, and disaccharide analysis of heparinase-catalyzed depolymerized heparin.
27084870	7	6	theme	porcine	966:972	arg1	heparin					985:991	porcine intestinal heparin	966:991	porcine intestinal heparin	966:991	The current study examines bovine heparins prepared from both intestines and lung and compares these to porcine intestinal heparin.
27084870	5	7	theme	intestinal	721:730	arg1	heparin					732:738	porcine intestinal heparin	713:738	porcine intestinal heparin	713:738	Other sources of heparin including bovine intestine and bovine lung are being examined as potential substitutes for porcine intestinal heparin.
27084870	5	8	theme	bovine	653:658	arg1	lung					660:663	bovine lung	653:663	bovine lung	653:663	Other sources of heparin including bovine intestine and bovine lung are being examined as potential substitutes for porcine intestinal heparin.
27084870	5	9	theme	Other	597:601	arg1	lung					660:663	bovine lung	653:663	bovine lung	653:663	Other sources of heparin including bovine intestine and bovine lung are being examined as potential substitutes for porcine intestinal heparin.
27084870	5	9	theme	Other	597:601	arg1	intestine					639:647	bovine intestine	632:647	bovine intestine	632:647	Other sources of heparin including bovine intestine and bovine lung are being examined as potential substitutes for porcine intestinal heparin.
27084870	5	9	theme	Other	597:601	arg1	sources					603:609	Other sources	597:609	Other sources of heparin including bovine intestine and bovine lung	597:663	Other sources of heparin including bovine intestine and bovine lung are being examined as potential substitutes for porcine intestinal heparin.
27084870	2	10	theme	trisulfated	254:264	arg1	disaccharides					266:278	trisulfated disaccharides	254:278	trisulfated disaccharides comprised of 1,4-linked iduronic acid and glucosamine residues containing some glucuronic acid residues	254:382	It is also a linear glycosaminoglycan with an average mass between 10 and 20 kDa and is primarily made up of trisulfated disaccharides comprised of 1,4-linked iduronic acid and glucosamine residues containing some glucuronic acid residues.
27084870	10	11	theme	porcine	1396:1402	arg1	heparins					1404:1411	bovine and porcine heparins	1385:1411	bovine and porcine heparins	1385:1411	The goal of this study is to establish the structural and functional similarities and potential differences between bovine and porcine heparins.
27084870	4	12	theme	porcine	577:583	arg1	intestines					585:594	porcine intestines	577:594	porcine intestines	577:594	Pharmaceutical heparin currently used in the United States is primarily extracted from porcine intestines.
27084870	3	13	located	found	447:451	arg1	intestines					467:476	intestines	467:476	intestines	467:476	Heparin is biosynthesized in the Golgi of mast cells commonly found in the liver, intestines, and lungs.
27084870	3	13	located	found	447:451	arg1	lungs					483:487	lungs	483:487	lungs	483:487	Heparin is biosynthesized in the Golgi of mast cells commonly found in the liver, intestines, and lungs.
27084870	3	13	located	found	447:451	arg1	liver					460:464	liver	460:464	liver	460:464	Heparin is biosynthesized in the Golgi of mast cells commonly found in the liver, intestines, and lungs.
27084870	3	13	located	found	447:451	arg2	cells					432:436	mast cells	427:436	mast cells commonly found in the liver, intestines, and lungs	427:487	Heparin is biosynthesized in the Golgi of mast cells commonly found in the liver, intestines, and lungs.
27084870	2	14	theme	iduronic	304:311	arg1	acid					313:316	1,4-linked iduronic acid and glucosamine residues	293:341	acid	313:316	It is also a linear glycosaminoglycan with an average mass between 10 and 20 kDa and is primarily made up of trisulfated disaccharides comprised of 1,4-linked iduronic acid and glucosamine residues containing some glucuronic acid residues.
27084870	11	15	theme	structural	1447:1456	arg1	similarities					1476:1487	similarities	1476:1487	similarities	1476:1487	Porcine and bovine heparins have structural and compositional similarities and differences.
27084870	2	16	contain	containing	343:352	arg1	acid					313:316	1,4-linked iduronic acid and glucosamine residues	293:341	acid	313:316	It is also a linear glycosaminoglycan with an average mass between 10 and 20 kDa and is primarily made up of trisulfated disaccharides comprised of 1,4-linked iduronic acid and glucosamine residues containing some glucuronic acid residues.
27084870	2	16	contain	containing	343:352	arg2	residues					375:382	some glucuronic acid residues	354:382	some glucuronic acid residues	354:382	It is also a linear glycosaminoglycan with an average mass between 10 and 20 kDa and is primarily made up of trisulfated disaccharides comprised of 1,4-linked iduronic acid and glucosamine residues containing some glucuronic acid residues.
27084870	7	17	theme	current	866:872	arg1	study					874:878	The current study	862:878	The current study	862:878	The current study examines bovine heparins prepared from both intestines and lung and compares these to porcine intestinal heparin.
27084870	0	18	attach	Derived	21:27	arg1	Tissues					41:47	Bovine Tissues	34:47	Bovine Tissues	34:47	Analysis of Heparins Derived From Bovine Tissues and Comparison to Porcine Intestinal Heparins.
27084870	0	18	attach	Derived	21:27	arg2	Heparins					12:19	Heparins	12:19	Heparins Derived From Bovine Tissues	12:47	Analysis of Heparins Derived From Bovine Tissues and Comparison to Porcine Intestinal Heparins.
27084870	10	19	theme	structural	1312:1321	arg1	similarities					1338:1349	similarities	1338:1349	similarities	1338:1349	The goal of this study is to establish the structural and functional similarities and potential differences between bovine and porcine heparins.
27084870	3	20	theme	mast	427:430	arg1	cells					432:436	mast cells	427:436	mast cells commonly found in the liver, intestines, and lungs	427:487	Heparin is biosynthesized in the Golgi of mast cells commonly found in the liver, intestines, and lungs.
27084870	5	21	theme	heparin	614:620	arg1	lung					660:663	bovine lung	653:663	bovine lung	653:663	Other sources of heparin including bovine intestine and bovine lung are being examined as potential substitutes for porcine intestinal heparin.
27084870	5	21	theme	heparin	614:620	arg1	intestine					639:647	bovine intestine	632:647	bovine intestine	632:647	Other sources of heparin including bovine intestine and bovine lung are being examined as potential substitutes for porcine intestinal heparin.
27084870	5	21	theme	heparin	614:620	arg1	sources					603:609	Other sources	597:609	Other sources of heparin including bovine intestine and bovine lung	597:663	Other sources of heparin including bovine intestine and bovine lung are being examined as potential substitutes for porcine intestinal heparin.
27084870	3	22	theme	cells	432:436	arg1	Golgi					418:422	the Golgi	414:422	the Golgi of mast cells commonly found in the liver, intestines, and lungs	414:487	Heparin is biosynthesized in the Golgi of mast cells commonly found in the liver, intestines, and lungs.
27084870	8	23	theme	heparin	1180:1186	arg1	chromatography					1100:1113	gel permeation chromatography	1085:1113	gel permeation chromatography	1085:1113	The structural properties of these heparins are examined using nuclear magnetic resonance, gel permeation chromatography, and disaccharide analysis of heparinase-catalyzed depolymerized heparin.
27084870	8	23	theme	heparin	1180:1186	arg1	analysis					1133:1140	disaccharide analysis	1120:1140	disaccharide analysis of heparinase-catalyzed depolymerized heparin	1120:1186	The structural properties of these heparins are examined using nuclear magnetic resonance, gel permeation chromatography, and disaccharide analysis of heparinase-catalyzed depolymerized heparin.
27084870	8	23	theme	heparin	1180:1186	arg1	resonance					1074:1082	nuclear magnetic resonance	1057:1082	nuclear magnetic resonance	1057:1082	The structural properties of these heparins are examined using nuclear magnetic resonance, gel permeation chromatography, and disaccharide analysis of heparinase-catalyzed depolymerized heparin.
27084870	8	24	theme	structural	998:1007	arg1	properties					1009:1018	The structural properties	994:1018	The structural properties of these heparins	994:1036	The structural properties of these heparins are examined using nuclear magnetic resonance, gel permeation chromatography, and disaccharide analysis of heparinase-catalyzed depolymerized heparin.
27084870	2	25	theme	1,4-linked	293:302	arg1	acid					313:316	1,4-linked iduronic acid and glucosamine residues	293:341	acid	313:316	It is also a linear glycosaminoglycan with an average mass between 10 and 20 kDa and is primarily made up of trisulfated disaccharides comprised of 1,4-linked iduronic acid and glucosamine residues containing some glucuronic acid residues.
27084870	4	26	theme	United	535:540	arg1	States					542:547	the United States	531:547	the United States	531:547	Pharmaceutical heparin currently used in the United States is primarily extracted from porcine intestines.
27084870	5	27	theme	bovine	632:637	arg1	intestine					639:647	bovine intestine	632:647	bovine intestine	632:647	Other sources of heparin including bovine intestine and bovine lung are being examined as potential substitutes for porcine intestinal heparin.
27084870	8	28	theme	magnetic	1065:1072	arg1	resonance					1074:1082	nuclear magnetic resonance	1057:1082	nuclear magnetic resonance	1057:1082	The structural properties of these heparins are examined using nuclear magnetic resonance, gel permeation chromatography, and disaccharide analysis of heparinase-catalyzed depolymerized heparin.
27084870	10	29	theme	potential	1355:1363	arg1	differences					1365:1375	potential differences	1355:1375	potential differences	1355:1375	The goal of this study is to establish the structural and functional similarities and potential differences between bovine and porcine heparins.
27084870	2	30	theme	acid	370:373	arg1	residues					375:382	some glucuronic acid residues	354:382	some glucuronic acid residues	354:382	It is also a linear glycosaminoglycan with an average mass between 10 and 20 kDa and is primarily made up of trisulfated disaccharides comprised of 1,4-linked iduronic acid and glucosamine residues containing some glucuronic acid residues.
27084870	8	31	theme	permeation	1089:1098	arg1	chromatography					1100:1113	gel permeation chromatography	1085:1113	gel permeation chromatography	1085:1113	The structural properties of these heparins are examined using nuclear magnetic resonance, gel permeation chromatography, and disaccharide analysis of heparinase-catalyzed depolymerized heparin.
27084870	9	32	theme	heparins	1233:1240	arg1	activities					1213:1222	The in vitro functional activities	1189:1222	The in vitro functional activities of these heparins	1189:1240	The in vitro functional activities of these heparins have also been determined.
27084870	11	33	contain	have	1442:1445	arg2	similarities					1476:1487	similarities	1476:1487	similarities	1476:1487	Porcine and bovine heparins have structural and compositional similarities and differences.
27084870	11	33	contain	have	1442:1445	arg1	heparins					1433:1440	bovine heparins	1426:1440	bovine heparins	1426:1440	Porcine and bovine heparins have structural and compositional similarities and differences.
27084870	11	33	contain	have	1442:1445	arg2	differences					1493:1503	differences	1493:1503	differences	1493:1503	Porcine and bovine heparins have structural and compositional similarities and differences.
27084870	11	33	contain	have	1442:1445	arg1	Porcine					1414:1420	Porcine	1414:1420	Porcine	1414:1420	Porcine and bovine heparins have structural and compositional similarities and differences.
27084870	0	34	theme	Heparins	12:19	arg1	Analysis					0:7	Analysis	0:7	Analysis of Heparins Derived From Bovine Tissues	0:47	Analysis of Heparins Derived From Bovine Tissues and Comparison to Porcine Intestinal Heparins.
27084870	0	34	theme	Heparins	12:19	arg1	Comparison					53:62	Comparison	53:62	Comparison to Porcine Intestinal Heparins	53:93	Analysis of Heparins Derived From Bovine Tissues and Comparison to Porcine Intestinal Heparins.
27084870	8	35	theme	gel	1085:1087	arg1	chromatography					1100:1113	gel permeation chromatography	1085:1113	gel permeation chromatography	1085:1113	The structural properties of these heparins are examined using nuclear magnetic resonance, gel permeation chromatography, and disaccharide analysis of heparinase-catalyzed depolymerized heparin.
27084870	10	36	theme	functional	1327:1336	arg1	similarities					1338:1349	similarities	1338:1349	similarities	1338:1349	The goal of this study is to establish the structural and functional similarities and potential differences between bovine and porcine heparins.
27084870	0	37	theme	Bovine	34:39	arg1	Tissues					41:47	Bovine Tissues	34:47	Bovine Tissues	34:47	Analysis of Heparins Derived From Bovine Tissues and Comparison to Porcine Intestinal Heparins.
27084870	8	38	theme	disaccharide	1120:1131	arg1	analysis					1133:1140	disaccharide analysis	1120:1140	disaccharide analysis of heparinase-catalyzed depolymerized heparin	1120:1186	The structural properties of these heparins are examined using nuclear magnetic resonance, gel permeation chromatography, and disaccharide analysis of heparinase-catalyzed depolymerized heparin.
27084870	2	39	theme	linear	158:163	arg1	glycosaminoglycan					165:181	a linear glycosaminoglycan	156:181	a linear glycosaminoglycan with an average mass between 10 and 20 kDa	156:224	It is also a linear glycosaminoglycan with an average mass between 10 and 20 kDa and is primarily made up of trisulfated disaccharides comprised of 1,4-linked iduronic acid and glucosamine residues containing some glucuronic acid residues.
27084870	2	39	theme	linear	158:163	arg1	It					145:146	It	145:146	It	145:146	It is also a linear glycosaminoglycan with an average mass between 10 and 20 kDa and is primarily made up of trisulfated disaccharides comprised of 1,4-linked iduronic acid and glucosamine residues containing some glucuronic acid residues.
27084870	9	40	dep	in	1193:1194	arg1	vitro					1196:1200	vitro	1196:1200	vitro	1196:1200	The in vitro functional activities of these heparins have also been determined.
27084870	11	41	theme	bovine	1426:1431	arg1	heparins					1433:1440	bovine heparins	1426:1440	bovine heparins	1426:1440	Porcine and bovine heparins have structural and compositional similarities and differences.
27084870	5	42	theme	porcine	713:719	arg1	heparin					732:738	porcine intestinal heparin	713:738	porcine intestinal heparin	713:738	Other sources of heparin including bovine intestine and bovine lung are being examined as potential substitutes for porcine intestinal heparin.
27084870	11	43	theme	compositional	1462:1474	arg1	similarities					1476:1487	similarities	1476:1487	similarities	1476:1487	Porcine and bovine heparins have structural and compositional similarities and differences.
27084870	6	44	theme	heparin	805:811	arg1	supply					813:818	the heparin supply	801:818	the heparin supply	801:818	These additional sources are intended to serve to diversify the heparin supply, making this lifesaving drug more secure.
27084870	10	45	theme	bovine	1385:1390	arg1	heparins					1404:1411	bovine and porcine heparins	1385:1411	bovine and porcine heparins	1385:1411	The goal of this study is to establish the structural and functional similarities and potential differences between bovine and porcine heparins.
27084870	2	46	theme	glucosamine	322:332	arg1	residues					334:341	1,4-linked iduronic acid and glucosamine residues	293:341	residues	334:341	It is also a linear glycosaminoglycan with an average mass between 10 and 20 kDa and is primarily made up of trisulfated disaccharides comprised of 1,4-linked iduronic acid and glucosamine residues containing some glucuronic acid residues.
27084870	2	47	link	1,4-linked	293:302	arg1	acid					313:316	1,4-linked iduronic acid and glucosamine residues	293:341	acid	313:316	It is also a linear glycosaminoglycan with an average mass between 10 and 20 kDa and is primarily made up of trisulfated disaccharides comprised of 1,4-linked iduronic acid and glucosamine residues containing some glucuronic acid residues.
27084870	6	48	theme	lifesaving	833:842	arg1	drug					844:847	this lifesaving drug	828:847	this lifesaving drug	828:847	These additional sources are intended to serve to diversify the heparin supply, making this lifesaving drug more secure.
27084870	8	49	theme	nuclear	1057:1063	arg1	resonance					1074:1082	nuclear magnetic resonance	1057:1082	nuclear magnetic resonance	1057:1082	The structural properties of these heparins are examined using nuclear magnetic resonance, gel permeation chromatography, and disaccharide analysis of heparinase-catalyzed depolymerized heparin.
27084870	2	50	theme	average	191:197	arg1	mass					199:202	an average mass	188:202	an average mass between 10 and 20 kDa	188:224	It is also a linear glycosaminoglycan with an average mass between 10 and 20 kDa and is primarily made up of trisulfated disaccharides comprised of 1,4-linked iduronic acid and glucosamine residues containing some glucuronic acid residues.
27084870	6	51	theme	additional	747:756	arg1	sources					758:764	These additional sources	741:764	These additional sources	741:764	These additional sources are intended to serve to diversify the heparin supply, making this lifesaving drug more secure.
27084870	1	52	theme	used	116:119	arg1	Heparin					96:102	Heparin	96:102	Heparin	96:102	Heparin is a widely used clinical anticoagulant.
27084870	1	52	theme	used	116:119	arg1	anticoagulant					130:142	a widely used clinical anticoagulant	107:142	a widely used clinical anticoagulant	107:142	Heparin is a widely used clinical anticoagulant.
27084870	10	53	theme	study	1286:1290	arg1	goal					1273:1276	The goal	1269:1276	The goal of this study	1269:1290	The goal of this study is to establish the structural and functional similarities and potential differences between bovine and porcine heparins.
27084870	3	54	dep	liver	460:464	arg1	the					456:458	the	456:458	the	456:458	Heparin is biosynthesized in the Golgi of mast cells commonly found in the liver, intestines, and lungs.
27084870	1	55	theme	clinical	121:128	arg1	Heparin					96:102	Heparin	96:102	Heparin	96:102	Heparin is a widely used clinical anticoagulant.
27084870	1	55	theme	clinical	121:128	arg1	anticoagulant					130:142	a widely used clinical anticoagulant	107:142	a widely used clinical anticoagulant	107:142	Heparin is a widely used clinical anticoagulant.
27084870	2	56	with	glycosaminoglycan	165:181	arg1	mass					199:202	an average mass	188:202	an average mass between 10 and 20 kDa	188:224	It is also a linear glycosaminoglycan with an average mass between 10 and 20 kDa and is primarily made up of trisulfated disaccharides comprised of 1,4-linked iduronic acid and glucosamine residues containing some glucuronic acid residues.
27084870	8	57	theme	depolymerized	1166:1178	arg1	heparin					1180:1186	heparinase-catalyzed depolymerized heparin	1145:1186	heparinase-catalyzed depolymerized heparin	1145:1186	The structural properties of these heparins are examined using nuclear magnetic resonance, gel permeation chromatography, and disaccharide analysis of heparinase-catalyzed depolymerized heparin.
27084870	9	58	theme	in	1193:1194	arg1	activities					1213:1222	The in vitro functional activities	1189:1222	The in vitro functional activities of these heparins	1189:1240	The in vitro functional activities of these heparins have also been determined.
27084870	2	59	theme	glucuronic	359:368	arg1	residues					375:382	some glucuronic acid residues	354:382	some glucuronic acid residues	354:382	It is also a linear glycosaminoglycan with an average mass between 10 and 20 kDa and is primarily made up of trisulfated disaccharides comprised of 1,4-linked iduronic acid and glucosamine residues containing some glucuronic acid residues.
27084870	8	60	theme	heparinase-catalyzed	1145:1164	arg1	heparin					1180:1186	heparinase-catalyzed depolymerized heparin	1145:1186	heparinase-catalyzed depolymerized heparin	1145:1186	The structural properties of these heparins are examined using nuclear magnetic resonance, gel permeation chromatography, and disaccharide analysis of heparinase-catalyzed depolymerized heparin.
27084870	7	61	theme	intestinal	974:983	arg1	heparin					985:991	porcine intestinal heparin	966:991	porcine intestinal heparin	966:991	The current study examines bovine heparins prepared from both intestines and lung and compares these to porcine intestinal heparin.
28686432	0	0	from	Mechanisms	24:33	arg1	Dynamics					93:100	Interfacial Dynamics	81:100	Interfacial Dynamics	81:100	Synergistic Reinforcing Mechanisms in Cellulose Nanofibrils Composite Hydrogels: Interfacial Dynamics, Energy Dissipation, and Damage Resistance.
28686432	0	0	from	Mechanisms	24:33	arg1	Nanofibrils					48:58	Cellulose Nanofibrils	38:58	Cellulose Nanofibrils	38:58	Synergistic Reinforcing Mechanisms in Cellulose Nanofibrils Composite Hydrogels: Interfacial Dynamics, Energy Dissipation, and Damage Resistance.
28686432	0	0	from	Mechanisms	24:33	arg1	Resistance					134:143	Damage Resistance	127:143	Damage Resistance	127:143	Synergistic Reinforcing Mechanisms in Cellulose Nanofibrils Composite Hydrogels: Interfacial Dynamics, Energy Dissipation, and Damage Resistance.
28686432	0	0	from	Mechanisms	24:33	arg1	Dissipation					110:120	Energy Dissipation	103:120	Energy Dissipation	103:120	Synergistic Reinforcing Mechanisms in Cellulose Nanofibrils Composite Hydrogels: Interfacial Dynamics, Energy Dissipation, and Damage Resistance.
28686432	7	1	theme	polymer	1146:1152	arg1	chains					1154:1159	the polymer chains	1142:1159	the polymer chains desorbed from the CNF surface under oscillatory shear	1142:1213	Moreover, the deformation-resting experiments show a unique interface stiffening mechanism where the polymer chains desorbed from the CNF surface under oscillatory shear become entangled during resting time.
28686432	10	2	theme	reinforcement	1602:1614	arg1	mechanism					1616:1624	microscopic reinforcement mechanism	1590:1624	microscopic reinforcement mechanism	1590:1624	These findings advance our understanding the role of interfacial layer in microscopic reinforcement mechanism and provide a constitutive foundation for exploring the deformation behaviors of the cellulosic hydrogels.
28686432	6	3	theme	groups	1018:1023	arg1	number					999:1004	the number	995:1004	the number of free -OH groups on the CNF surface	995:1042	The results indicate a decrease of the number of free -OH groups on the CNF surface.
28686432	2	4	theme	hydrogel	382:389	arg1	system					362:367	a model system	354:367	a model system of composite hydrogel reinforced with cellulose nanofibrils (CNFs), where the integration of reversible hydrogen bonds into a lightly covalently cross-linked polyacrylamide (PAAm) matrix	354:554	In this work, we study a model system of composite hydrogel reinforced with cellulose nanofibrils (CNFs), where the integration of reversible hydrogen bonds into a lightly covalently cross-linked polyacrylamide (PAAm) matrix.
28686432	5	5	from	interaction	809:819	arg1	surface					861:867	the surface	857:867	the surface of CNF	857:874	The CNF-PAAm interaction among physically adsorbed chains on the surface of CNF is examined as a function of CNF fraction by sum frequency generation spectroscopy.
28686432	5	6	theme	sum	921:923	arg1	spectroscopy					946:957	sum frequency generation spectroscopy	921:957	sum frequency generation spectroscopy	921:957	The CNF-PAAm interaction among physically adsorbed chains on the surface of CNF is examined as a function of CNF fraction by sum frequency generation spectroscopy.
28686432	10	7	theme	constitutive	1640:1651	arg1	foundation					1653:1662	a constitutive foundation	1638:1662	a constitutive foundation for exploring the deformation behaviors of the cellulosic hydrogels	1638:1730	These findings advance our understanding the role of interfacial layer in microscopic reinforcement mechanism and provide a constitutive foundation for exploring the deformation behaviors of the cellulosic hydrogels.
28686432	1	8	theme	elastomers	286:295	arg1	properties					272:281	the mechanical properties	257:281	the mechanical properties of elastomers and in particular soft hydrogels	257:328	Engineering reversible cross-links between nanoparticles and polymer matrix is a promising avenue to reinforce the mechanical properties of elastomers and in particular soft hydrogels.
28686432	3	9	with	networks	600:607	arg1	modulus					649:655	modulus	649:655	modulus	649:655	This approach yields the dual cross-linked networks with synergistically improved strength, modulus, and toughness.
28686432	3	9	with	networks	600:607	arg1	toughness					662:670	toughness	662:670	toughness	662:670	This approach yields the dual cross-linked networks with synergistically improved strength, modulus, and toughness.
28686432	3	9	with	networks	600:607	arg1	strength					639:646	synergistically improved strength	614:646	synergistically improved strength	614:646	This approach yields the dual cross-linked networks with synergistically improved strength, modulus, and toughness.
28686432	6	10	from	surface	1036:1042	arg1	decrease					983:990	a decrease	981:990	a decrease of the number of free -OH groups on the CNF surface	981:1042	The results indicate a decrease of the number of free -OH groups on the CNF surface.
28686432	5	11	theme	fraction	909:916	arg1	interaction					809:819	The CNF-PAAm interaction	796:819	The CNF-PAAm interaction among physically adsorbed chains on the surface of CNF	796:874	The CNF-PAAm interaction among physically adsorbed chains on the surface of CNF is examined as a function of CNF fraction by sum frequency generation spectroscopy.
28686432	5	11	theme	fraction	909:916	arg1	function					893:900	a function	891:900	a function of CNF fraction	891:916	The CNF-PAAm interaction among physically adsorbed chains on the surface of CNF is examined as a function of CNF fraction by sum frequency generation spectroscopy.
28686432	6	12	theme	free	1009:1012	arg1	groups					1018:1023	free -OH groups	1009:1023	free -OH groups	1009:1023	The results indicate a decrease of the number of free -OH groups on the CNF surface.
28686432	0	13	theme	Energy	103:108	arg1	Dissipation					110:120	Energy Dissipation	103:120	Energy Dissipation	103:120	Synergistic Reinforcing Mechanisms in Cellulose Nanofibrils Composite Hydrogels: Interfacial Dynamics, Energy Dissipation, and Damage Resistance.
28686432	4	14	theme	cross-links	718:728	arg1	nature					688:693	The reversible nature	673:693	The reversible nature of the hydrogen-bonded cross-links	673:728	The reversible nature of the hydrogen-bonded cross-links manifests a strong strain rate (έ) dependent dynamics properties.
28686432	10	15	theme	cellulosic	1711:1720	arg1	hydrogels					1722:1730	the cellulosic hydrogels	1707:1730	the cellulosic hydrogels	1707:1730	These findings advance our understanding the role of interfacial layer in microscopic reinforcement mechanism and provide a constitutive foundation for exploring the deformation behaviors of the cellulosic hydrogels.
28686432	9	16	theme	crack	1486:1490	arg1	resistance					1504:1513	the crack propagation resistance	1482:1513	the crack propagation resistance	1482:1513	The fibril pull-out process activates a series of dissipation mechanisms that increase the crack propagation resistance.
28686432	5	17	theme	CNF-PAAm	800:807	arg1	interaction					809:819	The CNF-PAAm interaction	796:819	The CNF-PAAm interaction among physically adsorbed chains on the surface of CNF	796:874	The CNF-PAAm interaction among physically adsorbed chains on the surface of CNF is examined as a function of CNF fraction by sum frequency generation spectroscopy.
28686432	5	17	theme	CNF-PAAm	800:807	arg1	function					893:900	a function	891:900	a function of CNF fraction	891:916	The CNF-PAAm interaction among physically adsorbed chains on the surface of CNF is examined as a function of CNF fraction by sum frequency generation spectroscopy.
28686432	4	18	theme	strain	749:754	arg1	έ					762:762	έ	762:762	έ	762:762	The reversible nature of the hydrogen-bonded cross-links manifests a strong strain rate (έ) dependent dynamics properties.
28686432	4	18	theme	strain	749:754	arg1	rate					756:759	a strong strain rate	740:759	a strong strain rate (έ)	740:763	The reversible nature of the hydrogen-bonded cross-links manifests a strong strain rate (έ) dependent dynamics properties.
28686432	7	19	theme	stiffening	1115:1124	arg1	mechanism					1126:1134	a unique interface stiffening mechanism	1096:1134	a unique interface stiffening mechanism where the polymer chains desorbed from the CNF surface under oscillatory shear become entangled during resting time	1096:1250	Moreover, the deformation-resting experiments show a unique interface stiffening mechanism where the polymer chains desorbed from the CNF surface under oscillatory shear become entangled during resting time.
28686432	5	20	theme	CNF	872:874	arg1	surface					861:867	the surface	857:867	the surface of CNF	857:874	The CNF-PAAm interaction among physically adsorbed chains on the surface of CNF is examined as a function of CNF fraction by sum frequency generation spectroscopy.
28686432	9	21	theme	propagation	1492:1502	arg1	resistance					1504:1513	the crack propagation resistance	1482:1513	the crack propagation resistance	1482:1513	The fibril pull-out process activates a series of dissipation mechanisms that increase the crack propagation resistance.
28686432	2	22	theme	polyacrylamide	527:540	arg1	matrix					549:554	a lightly covalently cross-linked polyacrylamide (PAAm) matrix	493:554	a lightly covalently cross-linked polyacrylamide (PAAm) matrix	493:554	In this work, we study a model system of composite hydrogel reinforced with cellulose nanofibrils (CNFs), where the integration of reversible hydrogen bonds into a lightly covalently cross-linked polyacrylamide (PAAm) matrix.
28686432	1	23	theme	polymer	207:213	arg1	matrix					215:220	polymer matrix	207:220	polymer matrix	207:220	Engineering reversible cross-links between nanoparticles and polymer matrix is a promising avenue to reinforce the mechanical properties of elastomers and in particular soft hydrogels.
28686432	0	24	theme	Reinforcing	12:22	arg1	Mechanisms					24:33	Synergistic Reinforcing Mechanisms	0:33	Synergistic Reinforcing Mechanisms in Cellulose Nanofibrils Composite Hydrogels: Interfacial Dynamics, Energy Dissipation, and Damage Resistance.	0:144	Synergistic Reinforcing Mechanisms in Cellulose Nanofibrils Composite Hydrogels: Interfacial Dynamics, Energy Dissipation, and Damage Resistance.
28686432	3	25	link	cross-linked	587:598	arg1	networks					600:607	the dual cross-linked networks	578:607	the dual cross-linked networks with synergistically improved strength, modulus, and toughness	578:670	This approach yields the dual cross-linked networks with synergistically improved strength, modulus, and toughness.
28686432	7	26	theme	unique	1098:1103	arg1	mechanism					1126:1134	a unique interface stiffening mechanism	1096:1134	a unique interface stiffening mechanism where the polymer chains desorbed from the CNF surface under oscillatory shear become entangled during resting time	1096:1250	Moreover, the deformation-resting experiments show a unique interface stiffening mechanism where the polymer chains desorbed from the CNF surface under oscillatory shear become entangled during resting time.
28686432	2	27	theme	cross-linked	514:525	arg1	matrix					549:554	a lightly covalently cross-linked polyacrylamide (PAAm) matrix	493:554	a lightly covalently cross-linked polyacrylamide (PAAm) matrix	493:554	In this work, we study a model system of composite hydrogel reinforced with cellulose nanofibrils (CNFs), where the integration of reversible hydrogen bonds into a lightly covalently cross-linked polyacrylamide (PAAm) matrix.
28686432	3	28	theme	dual	582:585	arg1	networks					600:607	the dual cross-linked networks	578:607	the dual cross-linked networks with synergistically improved strength, modulus, and toughness	578:670	This approach yields the dual cross-linked networks with synergistically improved strength, modulus, and toughness.
28686432	7	29	theme	oscillatory	1197:1207	arg1	shear					1209:1213	oscillatory shear	1197:1213	oscillatory shear	1197:1213	Moreover, the deformation-resting experiments show a unique interface stiffening mechanism where the polymer chains desorbed from the CNF surface under oscillatory shear become entangled during resting time.
28686432	0	30	theme	Synergistic	0:10	arg1	Mechanisms					24:33	Synergistic Reinforcing Mechanisms	0:33	Synergistic Reinforcing Mechanisms in Cellulose Nanofibrils Composite Hydrogels: Interfacial Dynamics, Energy Dissipation, and Damage Resistance.	0:144	Synergistic Reinforcing Mechanisms in Cellulose Nanofibrils Composite Hydrogels: Interfacial Dynamics, Energy Dissipation, and Damage Resistance.
28686432	1	31	theme	particular	304:313	arg1	hydrogels					320:328	particular soft hydrogels	304:328	particular soft hydrogels	304:328	Engineering reversible cross-links between nanoparticles and polymer matrix is a promising avenue to reinforce the mechanical properties of elastomers and in particular soft hydrogels.
28686432	7	32	theme	interface	1105:1113	arg1	mechanism					1126:1134	a unique interface stiffening mechanism	1096:1134	a unique interface stiffening mechanism where the polymer chains desorbed from the CNF surface under oscillatory shear become entangled during resting time	1096:1250	Moreover, the deformation-resting experiments show a unique interface stiffening mechanism where the polymer chains desorbed from the CNF surface under oscillatory shear become entangled during resting time.
28686432	10	33	theme	microscopic	1590:1600	arg1	mechanism					1616:1624	microscopic reinforcement mechanism	1590:1624	microscopic reinforcement mechanism	1590:1624	These findings advance our understanding the role of interfacial layer in microscopic reinforcement mechanism and provide a constitutive foundation for exploring the deformation behaviors of the cellulosic hydrogels.
28686432	3	34	theme	cross-linked	587:598	arg1	networks					600:607	the dual cross-linked networks	578:607	the dual cross-linked networks with synergistically improved strength, modulus, and toughness	578:670	This approach yields the dual cross-linked networks with synergistically improved strength, modulus, and toughness.
28686432	9	35	theme	mechanisms	1457:1466	arg1	series					1435:1440	a series	1433:1440	a series of dissipation mechanisms that increase the crack propagation resistance	1433:1513	The fibril pull-out process activates a series of dissipation mechanisms that increase the crack propagation resistance.
28686432	6	36	from	decrease	983:990	arg1	surface					1036:1042	the CNF surface	1028:1042	the CNF surface	1028:1042	The results indicate a decrease of the number of free -OH groups on the CNF surface.
28686432	1	37	theme	soft	315:318	arg1	hydrogels					320:328	particular soft hydrogels	304:328	particular soft hydrogels	304:328	Engineering reversible cross-links between nanoparticles and polymer matrix is a promising avenue to reinforce the mechanical properties of elastomers and in particular soft hydrogels.
28686432	10	38	theme	interfacial	1569:1579	arg1	layer					1581:1585	interfacial layer	1569:1585	interfacial layer	1569:1585	These findings advance our understanding the role of interfacial layer in microscopic reinforcement mechanism and provide a constitutive foundation for exploring the deformation behaviors of the cellulosic hydrogels.
28686432	4	39	theme	dynamics	775:782	arg1	properties					784:793	a strong strain rate (έ) dependent dynamics properties	740:793	a strong strain rate (έ) dependent dynamics properties	740:793	The reversible nature of the hydrogen-bonded cross-links manifests a strong strain rate (έ) dependent dynamics properties.
28686432	8	40	theme	CNF	1302:1304	arg1	slip					1318:1321	the CNF interfacial slip	1298:1321	the CNF interfacial slip	1298:1321	The bending micromechanics test reveals that the CNF interfacial slip imparts the capability to strengthen the composites during deformation.
28686432	7	41	theme	deformation-resting	1059:1077	arg1	experiments					1079:1089	the deformation-resting experiments	1055:1089	the deformation-resting experiments	1055:1089	Moreover, the deformation-resting experiments show a unique interface stiffening mechanism where the polymer chains desorbed from the CNF surface under oscillatory shear become entangled during resting time.
28686432	0	42	theme	Damage	127:132	arg1	Resistance					134:143	Damage Resistance	127:143	Damage Resistance	127:143	Synergistic Reinforcing Mechanisms in Cellulose Nanofibrils Composite Hydrogels: Interfacial Dynamics, Energy Dissipation, and Damage Resistance.
28686432	4	43	theme	dependent	765:773	arg1	properties					784:793	a strong strain rate (έ) dependent dynamics properties	740:793	a strong strain rate (έ) dependent dynamics properties	740:793	The reversible nature of the hydrogen-bonded cross-links manifests a strong strain rate (έ) dependent dynamics properties.
28686432	4	44	theme	reversible	677:686	arg1	nature					688:693	The reversible nature	673:693	The reversible nature of the hydrogen-bonded cross-links	673:728	The reversible nature of the hydrogen-bonded cross-links manifests a strong strain rate (έ) dependent dynamics properties.
28686432	1	45	theme	promising	227:235	arg1	avenue					237:242	a promising avenue	225:242	a promising avenue to reinforce the mechanical properties of elastomers and in particular soft hydrogels	225:328	Engineering reversible cross-links between nanoparticles and polymer matrix is a promising avenue to reinforce the mechanical properties of elastomers and in particular soft hydrogels.
28686432	5	46	theme	generation	935:944	arg1	spectroscopy					946:957	sum frequency generation spectroscopy	921:957	sum frequency generation spectroscopy	921:957	The CNF-PAAm interaction among physically adsorbed chains on the surface of CNF is examined as a function of CNF fraction by sum frequency generation spectroscopy.
28686432	6	47	from	number	999:1004	arg1	surface					1036:1042	the CNF surface	1028:1042	the CNF surface	1028:1042	The results indicate a decrease of the number of free -OH groups on the CNF surface.
28686432	10	48	theme	deformation	1682:1692	arg1	behaviors					1694:1702	the deformation behaviors	1678:1702	the deformation behaviors of the cellulosic hydrogels	1678:1730	These findings advance our understanding the role of interfacial layer in microscopic reinforcement mechanism and provide a constitutive foundation for exploring the deformation behaviors of the cellulosic hydrogels.
28686432	0	49	theme	Cellulose	38:46	arg1	Nanofibrils					48:58	Cellulose Nanofibrils	38:58	Cellulose Nanofibrils	38:58	Synergistic Reinforcing Mechanisms in Cellulose Nanofibrils Composite Hydrogels: Interfacial Dynamics, Energy Dissipation, and Damage Resistance.
28686432	9	50	theme	pull-out	1406:1413	arg1	process					1415:1421	The fibril pull-out process	1395:1421	The fibril pull-out process	1395:1421	The fibril pull-out process activates a series of dissipation mechanisms that increase the crack propagation resistance.
28686432	2	51	theme	bonds	482:486	arg1	integration					447:457	the integration	443:457	the integration of reversible hydrogen bonds into a lightly covalently cross-linked polyacrylamide (PAAm) matrix	443:554	In this work, we study a model system of composite hydrogel reinforced with cellulose nanofibrils (CNFs), where the integration of reversible hydrogen bonds into a lightly covalently cross-linked polyacrylamide (PAAm) matrix.
28686432	6	52	theme	-OH	1014:1016	arg1	groups					1018:1023	free -OH groups	1009:1023	free -OH groups	1009:1023	The results indicate a decrease of the number of free -OH groups on the CNF surface.
28686432	3	53	theme	improved	630:637	arg1	strength					639:646	synergistically improved strength	614:646	synergistically improved strength	614:646	This approach yields the dual cross-linked networks with synergistically improved strength, modulus, and toughness.
28686432	8	54	theme	interfacial	1306:1316	arg1	slip					1318:1321	the CNF interfacial slip	1298:1321	the CNF interfacial slip	1298:1321	The bending micromechanics test reveals that the CNF interfacial slip imparts the capability to strengthen the composites during deformation.
28686432	2	55	theme	hydrogen	473:480	arg1	bonds					482:486	reversible hydrogen bonds	462:486	reversible hydrogen bonds	462:486	In this work, we study a model system of composite hydrogel reinforced with cellulose nanofibrils (CNFs), where the integration of reversible hydrogen bonds into a lightly covalently cross-linked polyacrylamide (PAAm) matrix.
28686432	5	56	theme	CNF	905:907	arg1	fraction					909:916	CNF fraction	905:916	CNF fraction	905:916	The CNF-PAAm interaction among physically adsorbed chains on the surface of CNF is examined as a function of CNF fraction by sum frequency generation spectroscopy.
28686432	5	57	theme	adsorbed	838:845	arg1	chains					847:852	physically adsorbed chains	827:852	physically adsorbed chains	827:852	The CNF-PAAm interaction among physically adsorbed chains on the surface of CNF is examined as a function of CNF fraction by sum frequency generation spectroscopy.
28686432	7	58	theme	resting	1239:1245	arg1	time					1247:1250	resting time	1239:1250	resting time	1239:1250	Moreover, the deformation-resting experiments show a unique interface stiffening mechanism where the polymer chains desorbed from the CNF surface under oscillatory shear become entangled during resting time.
28686432	0	59	dep	Nanofibrils	48:58	arg1	Hydrogels					70:78	Composite Hydrogels	60:78	Composite Hydrogels	60:78	Synergistic Reinforcing Mechanisms in Cellulose Nanofibrils Composite Hydrogels: Interfacial Dynamics, Energy Dissipation, and Damage Resistance.
28686432	2	60	theme	model	356:360	arg1	system					362:367	a model system	354:367	a model system of composite hydrogel reinforced with cellulose nanofibrils (CNFs), where the integration of reversible hydrogen bonds into a lightly covalently cross-linked polyacrylamide (PAAm) matrix	354:554	In this work, we study a model system of composite hydrogel reinforced with cellulose nanofibrils (CNFs), where the integration of reversible hydrogen bonds into a lightly covalently cross-linked polyacrylamide (PAAm) matrix.
28686432	0	61	theme	Composite	60:68	arg1	Hydrogels					70:78	Composite Hydrogels	60:78	Composite Hydrogels	60:78	Synergistic Reinforcing Mechanisms in Cellulose Nanofibrils Composite Hydrogels: Interfacial Dynamics, Energy Dissipation, and Damage Resistance.
28686432	1	62	from	properties	272:281	arg1	hydrogels					320:328	particular soft hydrogels	304:328	particular soft hydrogels	304:328	Engineering reversible cross-links between nanoparticles and polymer matrix is a promising avenue to reinforce the mechanical properties of elastomers and in particular soft hydrogels.
28686432	2	63	theme	reversible	462:471	arg1	bonds					482:486	reversible hydrogen bonds	462:486	reversible hydrogen bonds	462:486	In this work, we study a model system of composite hydrogel reinforced with cellulose nanofibrils (CNFs), where the integration of reversible hydrogen bonds into a lightly covalently cross-linked polyacrylamide (PAAm) matrix.
28686432	2	64	link	cross-linked	514:525	arg1	matrix					549:554	a lightly covalently cross-linked polyacrylamide (PAAm) matrix	493:554	a lightly covalently cross-linked polyacrylamide (PAAm) matrix	493:554	In this work, we study a model system of composite hydrogel reinforced with cellulose nanofibrils (CNFs), where the integration of reversible hydrogen bonds into a lightly covalently cross-linked polyacrylamide (PAAm) matrix.
28686432	4	65	theme	strong	742:747	arg1	έ					762:762	έ	762:762	έ	762:762	The reversible nature of the hydrogen-bonded cross-links manifests a strong strain rate (έ) dependent dynamics properties.
28686432	4	65	theme	strong	742:747	arg1	rate					756:759	a strong strain rate	740:759	a strong strain rate (έ)	740:763	The reversible nature of the hydrogen-bonded cross-links manifests a strong strain rate (έ) dependent dynamics properties.
28686432	7	66	theme	CNF	1179:1181	arg1	surface					1183:1189	the CNF surface	1175:1189	the CNF surface	1175:1189	Moreover, the deformation-resting experiments show a unique interface stiffening mechanism where the polymer chains desorbed from the CNF surface under oscillatory shear become entangled during resting time.
28686432	9	67	theme	fibril	1399:1404	arg1	process					1415:1421	The fibril pull-out process	1395:1421	The fibril pull-out process	1395:1421	The fibril pull-out process activates a series of dissipation mechanisms that increase the crack propagation resistance.
28686432	10	68	theme	layer	1581:1585	arg1	understanding					1543:1555	our understanding the role	1539:1564	our understanding the role of interfacial layer	1539:1585	These findings advance our understanding the role of interfacial layer in microscopic reinforcement mechanism and provide a constitutive foundation for exploring the deformation behaviors of the cellulosic hydrogels.
28686432	5	69	theme	frequency	925:933	arg1	spectroscopy					946:957	sum frequency generation spectroscopy	921:957	sum frequency generation spectroscopy	921:957	The CNF-PAAm interaction among physically adsorbed chains on the surface of CNF is examined as a function of CNF fraction by sum frequency generation spectroscopy.
28686432	10	70	dep	understanding	1543:1555	arg1	role					1561:1564	the role	1557:1564	our understanding the role of interfacial layer	1539:1585	These findings advance our understanding the role of interfacial layer in microscopic reinforcement mechanism and provide a constitutive foundation for exploring the deformation behaviors of the cellulosic hydrogels.
28686432	9	71	theme	dissipation	1445:1455	arg1	mechanisms					1457:1466	dissipation mechanisms	1445:1466	dissipation mechanisms that increase the crack propagation resistance	1445:1513	The fibril pull-out process activates a series of dissipation mechanisms that increase the crack propagation resistance.
28686432	4	72	theme	hydrogen-bonded	702:716	arg1	cross-links					718:728	the hydrogen-bonded cross-links	698:728	the hydrogen-bonded cross-links	698:728	The reversible nature of the hydrogen-bonded cross-links manifests a strong strain rate (έ) dependent dynamics properties.
28686432	6	73	theme	CNF	1032:1034	arg1	surface					1036:1042	the CNF surface	1028:1042	the CNF surface	1028:1042	The results indicate a decrease of the number of free -OH groups on the CNF surface.
28686432	0	74	theme	Interfacial	81:91	arg1	Dynamics					93:100	Interfacial Dynamics	81:100	Interfacial Dynamics	81:100	Synergistic Reinforcing Mechanisms in Cellulose Nanofibrils Composite Hydrogels: Interfacial Dynamics, Energy Dissipation, and Damage Resistance.
28686432	1	75	theme	reversible	158:167	arg1	cross-links					169:179	reversible cross-links	158:179	reversible cross-links between nanoparticles and polymer matrix	158:220	Engineering reversible cross-links between nanoparticles and polymer matrix is a promising avenue to reinforce the mechanical properties of elastomers and in particular soft hydrogels.
28686432	2	76	theme	cellulose	407:415	arg1	CNFs					430:433	CNFs	430:433	CNFs	430:433	In this work, we study a model system of composite hydrogel reinforced with cellulose nanofibrils (CNFs), where the integration of reversible hydrogen bonds into a lightly covalently cross-linked polyacrylamide (PAAm) matrix.
28686432	2	76	theme	cellulose	407:415	arg1	nanofibrils					417:427	cellulose nanofibrils	407:427	cellulose nanofibrils (CNFs)	407:434	In this work, we study a model system of composite hydrogel reinforced with cellulose nanofibrils (CNFs), where the integration of reversible hydrogen bonds into a lightly covalently cross-linked polyacrylamide (PAAm) matrix.
28686432	1	77	theme	mechanical	261:270	arg1	properties					272:281	the mechanical properties	257:281	the mechanical properties of elastomers and in particular soft hydrogels	257:328	Engineering reversible cross-links between nanoparticles and polymer matrix is a promising avenue to reinforce the mechanical properties of elastomers and in particular soft hydrogels.
28686432	8	78	theme	bending	1257:1263	arg1	test					1280:1283	The bending micromechanics test	1253:1283	The bending micromechanics test	1253:1283	The bending micromechanics test reveals that the CNF interfacial slip imparts the capability to strengthen the composites during deformation.
28686432	8	79	theme	micromechanics	1265:1278	arg1	test					1280:1283	The bending micromechanics test	1253:1283	The bending micromechanics test	1253:1283	The bending micromechanics test reveals that the CNF interfacial slip imparts the capability to strengthen the composites during deformation.
28686432	6	80	theme	number	999:1004	arg1	decrease					983:990	a decrease	981:990	a decrease of the number of free -OH groups on the CNF surface	981:1042	The results indicate a decrease of the number of free -OH groups on the CNF surface.
28686432	2	81	theme	composite	372:380	arg1	hydrogel					382:389	composite hydrogel	372:389	composite hydrogel reinforced with cellulose nanofibrils (CNFs), where the integration of reversible hydrogen bonds into a lightly covalently cross-linked polyacrylamide (PAAm) matrix	372:554	In this work, we study a model system of composite hydrogel reinforced with cellulose nanofibrils (CNFs), where the integration of reversible hydrogen bonds into a lightly covalently cross-linked polyacrylamide (PAAm) matrix.
28686432	10	82	theme	hydrogels	1722:1730	arg1	behaviors					1694:1702	the deformation behaviors	1678:1702	the deformation behaviors of the cellulosic hydrogels	1678:1730	These findings advance our understanding the role of interfacial layer in microscopic reinforcement mechanism and provide a constitutive foundation for exploring the deformation behaviors of the cellulosic hydrogels.
28686432	2	83	theme	PAAm	543:546	arg1	matrix					549:554	a lightly covalently cross-linked polyacrylamide (PAAm) matrix	493:554	a lightly covalently cross-linked polyacrylamide (PAAm) matrix	493:554	In this work, we study a model system of composite hydrogel reinforced with cellulose nanofibrils (CNFs), where the integration of reversible hydrogen bonds into a lightly covalently cross-linked polyacrylamide (PAAm) matrix.
26428187	0	0	theme	tissue	90:95	arg1	engineering					97:107	bone tissue engineering	85:107	bone tissue engineering	85:107	Fabrication and in vitro biological activity of βTCP-Chitosan-Fucoidan composite for bone tissue engineering.
26428187	0	1	theme	bone	85:88	arg1	engineering					97:107	bone tissue engineering	85:107	bone tissue engineering	85:107	Fabrication and in vitro biological activity of βTCP-Chitosan-Fucoidan composite for bone tissue engineering.
26428187	4	2	theme	simulated	599:607	arg1	fluid					614:618	simulated body fluid	599:618	simulated body fluid	599:618	Biomineralization of scaffolds was evaluated by soaking them in simulated body fluid and the results revealed that the addition of Fucoidan into the scaffolds enhanced the formation of apatite layer on the surface of biocomposite after 7 days of immersion.
26428187	7	3	theme	potential	1139:1147	arg1	TCP-Ch-Fu					1118:1126	TCP-Ch-Fu	1118:1126	TCP-Ch-Fu	1118:1126	Thus, TCP-Ch-Fu could be a potential candidate for bone-tissue engineering applications.
26428187	7	3	theme	potential	1139:1147	arg1	candidate					1149:1157	a potential candidate	1137:1157	a potential candidate for bone-tissue engineering applications	1137:1198	Thus, TCP-Ch-Fu could be a potential candidate for bone-tissue engineering applications.
26428187	6	4	theme	cells	1085:1089	arg1	differentiation					1040:1054	osteogenic differentiation	1029:1054	osteogenic differentiation of human mesenchymal stromal cells in in vitro culture	1029:1109	The addition of Fucoidan to TCP-Ch increased the release of osteocalcin, confirming that it can support osteogenic differentiation of human mesenchymal stromal cells in in vitro culture.
26428187	5	5	theme	Blue	799:802	arg1	assay					804:808	Alamar Blue assay	792:808	Alamar Blue assay	792:808	Alamar Blue assay confirmed that the cell viability of human-derived bone marrow stromal cell was superior in the TCP-Ch-Fuscaffold.
26428187	6	6	dep	in	1094:1095	arg1	vitro					1097:1101	vitro	1097:1101	vitro	1097:1101	The addition of Fucoidan to TCP-Ch increased the release of osteocalcin, confirming that it can support osteogenic differentiation of human mesenchymal stromal cells in in vitro culture.
26428187	6	7	theme	stromal	1077:1083	arg1	cells					1085:1089	human mesenchymal stromal cells	1059:1089	human mesenchymal stromal cells	1059:1089	The addition of Fucoidan to TCP-Ch increased the release of osteocalcin, confirming that it can support osteogenic differentiation of human mesenchymal stromal cells in in vitro culture.
26428187	5	8	from	superior	890:897	arg1	TCP-Ch-Fuscaffold					906:922	the TCP-Ch-Fuscaffold	902:922	the TCP-Ch-Fuscaffold	902:922	Alamar Blue assay confirmed that the cell viability of human-derived bone marrow stromal cell was superior in the TCP-Ch-Fuscaffold.
26428187	4	9	theme	Fucoidan	666:673	arg1	addition					654:661	the addition	650:661	the addition of Fucoidan into the scaffolds	650:692	Biomineralization of scaffolds was evaluated by soaking them in simulated body fluid and the results revealed that the addition of Fucoidan into the scaffolds enhanced the formation of apatite layer on the surface of biocomposite after 7 days of immersion.
26428187	4	10	theme	immersion	781:789	arg1	days					773:776	7 days	771:776	7 days of immersion	771:789	Biomineralization of scaffolds was evaluated by soaking them in simulated body fluid and the results revealed that the addition of Fucoidan into the scaffolds enhanced the formation of apatite layer on the surface of biocomposite after 7 days of immersion.
26428187	7	11	theme	engineering	1175:1185	arg1	applications					1187:1198	bone-tissue engineering applications	1163:1198	bone-tissue engineering applications	1163:1198	Thus, TCP-Ch-Fu could be a potential candidate for bone-tissue engineering applications.
26428187	6	12	theme	mesenchymal	1065:1075	arg1	cells					1085:1089	human mesenchymal stromal cells	1059:1089	human mesenchymal stromal cells	1059:1089	The addition of Fucoidan to TCP-Ch increased the release of osteocalcin, confirming that it can support osteogenic differentiation of human mesenchymal stromal cells in in vitro culture.
26428187	2	13	dep	spectroscopy	289:300	arg1	measurement					315:325	measurement	315:325	measurement	315:325	The fabricated biocomposite scaffolds were analyzed by spectroscopy and porosity measurement.
26428187	5	14	theme	cell	881:884	arg1	superior					890:897	superior	890:897	superior	890:897	Alamar Blue assay confirmed that the cell viability of human-derived bone marrow stromal cell was superior in the TCP-Ch-Fuscaffold.
26428187	5	14	theme	cell	881:884	arg1	viability					834:842	the cell viability	825:842	the cell viability of human-derived bone marrow stromal cell	825:884	Alamar Blue assay confirmed that the cell viability of human-derived bone marrow stromal cell was superior in the TCP-Ch-Fuscaffold.
26428187	3	15	theme	compression	387:397	arg1	test					399:402	compression test	387:402	compression test	387:402	The biomechanical properties of scaffolds were assessed by compression test and the results suggested that the incorporation of Fucoidan into the biocomposite improves the compression strength of scaffolds.
26428187	6	16	theme	osteocalcin	985:995	arg1	release					974:980	the release	970:980	the release of osteocalcin	970:995	The addition of Fucoidan to TCP-Ch increased the release of osteocalcin, confirming that it can support osteogenic differentiation of human mesenchymal stromal cells in in vitro culture.
26428187	4	17	theme	scaffolds	556:564	arg1	Biomineralization					535:551	Biomineralization	535:551	Biomineralization of scaffolds	535:564	Biomineralization of scaffolds was evaluated by soaking them in simulated body fluid and the results revealed that the addition of Fucoidan into the scaffolds enhanced the formation of apatite layer on the surface of biocomposite after 7 days of immersion.
26428187	1	18	theme	freeze-drying	209:221	arg1	technique					223:231	the freeze-drying technique	205:231	the freeze-drying technique	205:231	We developed tricalcium phosphate-chitosan-fucoidan biocomposite scaffold (TCP-Ch-Fu) by using the freeze-drying technique.
26428187	3	19	theme	Fucoidan	456:463	arg1	incorporation					439:451	the incorporation	435:451	the incorporation of Fucoidan into the biocomposite	435:485	The biomechanical properties of scaffolds were assessed by compression test and the results suggested that the incorporation of Fucoidan into the biocomposite improves the compression strength of scaffolds.
26428187	6	20	theme	human	1059:1063	arg1	cells					1085:1089	human mesenchymal stromal cells	1059:1089	human mesenchymal stromal cells	1059:1089	The addition of Fucoidan to TCP-Ch increased the release of osteocalcin, confirming that it can support osteogenic differentiation of human mesenchymal stromal cells in in vitro culture.
26428187	7	21	theme	bone-tissue	1163:1173	arg1	applications					1187:1198	bone-tissue engineering applications	1163:1198	bone-tissue engineering applications	1163:1198	Thus, TCP-Ch-Fu could be a potential candidate for bone-tissue engineering applications.
26428187	3	22	theme	compression	500:510	arg1	strength					512:519	the compression strength	496:519	the compression strength of scaffolds	496:532	The biomechanical properties of scaffolds were assessed by compression test and the results suggested that the incorporation of Fucoidan into the biocomposite improves the compression strength of scaffolds.
26428187	3	23	theme	biomechanical	332:344	arg1	properties					346:355	The biomechanical properties	328:355	The biomechanical properties of scaffolds	328:368	The biomechanical properties of scaffolds were assessed by compression test and the results suggested that the incorporation of Fucoidan into the biocomposite improves the compression strength of scaffolds.
26428187	5	24	theme	marrow	866:871	arg1	cell					881:884	human-derived bone marrow stromal cell	847:884	human-derived bone marrow stromal cell	847:884	Alamar Blue assay confirmed that the cell viability of human-derived bone marrow stromal cell was superior in the TCP-Ch-Fuscaffold.
26428187	0	25	theme	in	16:17	arg1	activity					36:43	in vitro biological activity	16:43	in vitro biological activity	16:43	Fabrication and in vitro biological activity of βTCP-Chitosan-Fucoidan composite for bone tissue engineering.
26428187	5	26	theme	human-derived	847:859	arg1	marrow					866:871	human-derived bone marrow	847:871	human-derived bone marrow stromal cell	847:884	Alamar Blue assay confirmed that the cell viability of human-derived bone marrow stromal cell was superior in the TCP-Ch-Fuscaffold.
26428187	6	27	theme	osteogenic	1029:1038	arg1	differentiation					1040:1054	osteogenic differentiation	1029:1054	osteogenic differentiation of human mesenchymal stromal cells in in vitro culture	1029:1109	The addition of Fucoidan to TCP-Ch increased the release of osteocalcin, confirming that it can support osteogenic differentiation of human mesenchymal stromal cells in in vitro culture.
26428187	4	28	theme	layer	728:732	arg1	formation					707:715	the formation	703:715	the formation of apatite layer on the surface of biocomposite	703:763	Biomineralization of scaffolds was evaluated by soaking them in simulated body fluid and the results revealed that the addition of Fucoidan into the scaffolds enhanced the formation of apatite layer on the surface of biocomposite after 7 days of immersion.
26428187	5	29	theme	Alamar	792:797	arg1	assay					804:808	Alamar Blue assay	792:808	Alamar Blue assay	792:808	Alamar Blue assay confirmed that the cell viability of human-derived bone marrow stromal cell was superior in the TCP-Ch-Fuscaffold.
26428187	4	30	from	formation	707:715	arg1	surface					741:747	the surface	737:747	the surface of biocomposite	737:763	Biomineralization of scaffolds was evaluated by soaking them in simulated body fluid and the results revealed that the addition of Fucoidan into the scaffolds enhanced the formation of apatite layer on the surface of biocomposite after 7 days of immersion.
26428187	3	31	theme	scaffolds	524:532	arg1	strength					512:519	the compression strength	496:519	the compression strength of scaffolds	496:532	The biomechanical properties of scaffolds were assessed by compression test and the results suggested that the incorporation of Fucoidan into the biocomposite improves the compression strength of scaffolds.
26428187	0	32	theme	biological	25:34	arg1	activity					36:43	in vitro biological activity	16:43	in vitro biological activity	16:43	Fabrication and in vitro biological activity of βTCP-Chitosan-Fucoidan composite for bone tissue engineering.
26428187	5	33	theme	bone	861:864	arg1	marrow					866:871	human-derived bone marrow	847:871	human-derived bone marrow stromal cell	847:884	Alamar Blue assay confirmed that the cell viability of human-derived bone marrow stromal cell was superior in the TCP-Ch-Fuscaffold.
26428187	4	34	theme	apatite	720:726	arg1	layer					728:732	apatite layer	720:732	apatite layer	720:732	Biomineralization of scaffolds was evaluated by soaking them in simulated body fluid and the results revealed that the addition of Fucoidan into the scaffolds enhanced the formation of apatite layer on the surface of biocomposite after 7 days of immersion.
26428187	0	35	theme	βTCP-Chitosan-Fucoidan	48:69	arg1	composite					71:79	βTCP-Chitosan-Fucoidan composite	48:79	βTCP-Chitosan-Fucoidan composite	48:79	Fabrication and in vitro biological activity of βTCP-Chitosan-Fucoidan composite for bone tissue engineering.
26428187	4	36	theme	body	609:612	arg1	fluid					614:618	simulated body fluid	599:618	simulated body fluid	599:618	Biomineralization of scaffolds was evaluated by soaking them in simulated body fluid and the results revealed that the addition of Fucoidan into the scaffolds enhanced the formation of apatite layer on the surface of biocomposite after 7 days of immersion.
26428187	5	37	theme	cell	829:832	arg1	superior					890:897	superior	890:897	superior	890:897	Alamar Blue assay confirmed that the cell viability of human-derived bone marrow stromal cell was superior in the TCP-Ch-Fuscaffold.
26428187	5	37	theme	cell	829:832	arg1	viability					834:842	the cell viability	825:842	the cell viability of human-derived bone marrow stromal cell	825:884	Alamar Blue assay confirmed that the cell viability of human-derived bone marrow stromal cell was superior in the TCP-Ch-Fuscaffold.
26428187	5	38	link	human-derived	847:859	arg1	marrow					866:871	human-derived bone marrow	847:871	human-derived bone marrow stromal cell	847:884	Alamar Blue assay confirmed that the cell viability of human-derived bone marrow stromal cell was superior in the TCP-Ch-Fuscaffold.
26428187	1	39	theme	tricalcium	123:132	arg1	TCP-Ch-Fu					185:193	TCP-Ch-Fu	185:193	TCP-Ch-Fu	185:193	We developed tricalcium phosphate-chitosan-fucoidan biocomposite scaffold (TCP-Ch-Fu) by using the freeze-drying technique.
26428187	1	39	theme	tricalcium	123:132	arg1	scaffold					175:182	tricalcium phosphate-chitosan-fucoidan biocomposite scaffold	123:182	tricalcium phosphate-chitosan-fucoidan biocomposite scaffold (TCP-Ch-Fu)	123:194	We developed tricalcium phosphate-chitosan-fucoidan biocomposite scaffold (TCP-Ch-Fu) by using the freeze-drying technique.
26428187	5	40	theme	stromal	873:879	arg1	cell					881:884	human-derived bone marrow stromal cell	847:884	human-derived bone marrow stromal cell	847:884	Alamar Blue assay confirmed that the cell viability of human-derived bone marrow stromal cell was superior in the TCP-Ch-Fuscaffold.
26428187	6	41	from	differentiation	1040:1054	arg1	culture					1103:1109	in vitro culture	1094:1109	in vitro culture	1094:1109	The addition of Fucoidan to TCP-Ch increased the release of osteocalcin, confirming that it can support osteogenic differentiation of human mesenchymal stromal cells in in vitro culture.
26428187	2	42	theme	biocomposite	249:260	arg1	scaffolds					262:270	The fabricated biocomposite scaffolds	234:270	The fabricated biocomposite scaffolds	234:270	The fabricated biocomposite scaffolds were analyzed by spectroscopy and porosity measurement.
26428187	1	43	theme	phosphate-chitosan-fucoidan	134:160	arg1	TCP-Ch-Fu					185:193	TCP-Ch-Fu	185:193	TCP-Ch-Fu	185:193	We developed tricalcium phosphate-chitosan-fucoidan biocomposite scaffold (TCP-Ch-Fu) by using the freeze-drying technique.
26428187	1	43	theme	phosphate-chitosan-fucoidan	134:160	arg1	scaffold					175:182	tricalcium phosphate-chitosan-fucoidan biocomposite scaffold	123:182	tricalcium phosphate-chitosan-fucoidan biocomposite scaffold (TCP-Ch-Fu)	123:194	We developed tricalcium phosphate-chitosan-fucoidan biocomposite scaffold (TCP-Ch-Fu) by using the freeze-drying technique.
26428187	2	44	theme	fabricated	238:247	arg1	scaffolds					262:270	The fabricated biocomposite scaffolds	234:270	The fabricated biocomposite scaffolds	234:270	The fabricated biocomposite scaffolds were analyzed by spectroscopy and porosity measurement.
26428187	5	45	from	TCP-Ch-Fuscaffold	906:922	arg1	viability					834:842	the cell viability	825:842	the cell viability of human-derived bone marrow stromal cell	825:884	Alamar Blue assay confirmed that the cell viability of human-derived bone marrow stromal cell was superior in the TCP-Ch-Fuscaffold.
26428187	5	45	from	TCP-Ch-Fuscaffold	906:922	arg1	superior					890:897	superior	890:897	superior	890:897	Alamar Blue assay confirmed that the cell viability of human-derived bone marrow stromal cell was superior in the TCP-Ch-Fuscaffold.
26428187	1	46	theme	biocomposite	162:173	arg1	TCP-Ch-Fu					185:193	TCP-Ch-Fu	185:193	TCP-Ch-Fu	185:193	We developed tricalcium phosphate-chitosan-fucoidan biocomposite scaffold (TCP-Ch-Fu) by using the freeze-drying technique.
26428187	1	46	theme	biocomposite	162:173	arg1	scaffold					175:182	tricalcium phosphate-chitosan-fucoidan biocomposite scaffold	123:182	tricalcium phosphate-chitosan-fucoidan biocomposite scaffold (TCP-Ch-Fu)	123:194	We developed tricalcium phosphate-chitosan-fucoidan biocomposite scaffold (TCP-Ch-Fu) by using the freeze-drying technique.
26428187	3	47	theme	scaffolds	360:368	arg1	properties					346:355	The biomechanical properties	328:355	The biomechanical properties of scaffolds	328:368	The biomechanical properties of scaffolds were assessed by compression test and the results suggested that the incorporation of Fucoidan into the biocomposite improves the compression strength of scaffolds.
26428187	0	48	dep	in	16:17	arg1	vitro					19:23	vitro	19:23	vitro	19:23	Fabrication and in vitro biological activity of βTCP-Chitosan-Fucoidan composite for bone tissue engineering.
26428187	6	49	theme	Fucoidan	941:948	arg1	addition					929:936	The addition	925:936	The addition of Fucoidan to TCP-Ch	925:958	The addition of Fucoidan to TCP-Ch increased the release of osteocalcin, confirming that it can support osteogenic differentiation of human mesenchymal stromal cells in in vitro culture.
26428187	0	50	theme	composite	71:79	arg1	activity					36:43	in vitro biological activity	16:43	in vitro biological activity	16:43	Fabrication and in vitro biological activity of βTCP-Chitosan-Fucoidan composite for bone tissue engineering.
26428187	0	50	theme	composite	71:79	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication	0:10	Fabrication and in vitro biological activity of βTCP-Chitosan-Fucoidan composite for bone tissue engineering.
26428187	4	51	theme	biocomposite	752:763	arg1	surface					741:747	the surface	737:747	the surface of biocomposite	737:763	Biomineralization of scaffolds was evaluated by soaking them in simulated body fluid and the results revealed that the addition of Fucoidan into the scaffolds enhanced the formation of apatite layer on the surface of biocomposite after 7 days of immersion.
26428187	6	52	theme	in	1094:1095	arg1	culture					1103:1109	in vitro culture	1094:1109	in vitro culture	1094:1109	The addition of Fucoidan to TCP-Ch increased the release of osteocalcin, confirming that it can support osteogenic differentiation of human mesenchymal stromal cells in in vitro culture.
25913882	0	0	theme	metabolites	198:208	arg1	production					210:219	biomass composition and metabolites production	174:219	biomass composition and metabolites production	174:219	Evaluation of batch and semi-continuous culture of Porphyridium purpureum in a photobioreactor in high latitudes using Fourier Transform Infrared spectroscopy for monitoring biomass composition and metabolites production.
25913882	4	1	from	exopolysaccharides	1192:1209	arg1	cultures					1227:1234	P. purpureum cultures	1214:1234	P. purpureum cultures	1214:1234	FTIR technology applied to microalgal production is a valuable and reliable tool to determine on a daily basis not just the evolution of macromolecules composition (lipids, carbohydrates and proteins) but also for the characterisation of the metabolites produced such as phycoerythrin or exopolysaccharides in P. purpureum cultures.
25913882	0	2	dep	Fourier	119:125	arg1	Transform					127:135	Transform	127:135	Transform Infrared spectroscopy for monitoring biomass composition and metabolites production	127:219	Evaluation of batch and semi-continuous culture of Porphyridium purpureum in a photobioreactor in high latitudes using Fourier Transform Infrared spectroscopy for monitoring biomass composition and metabolites production.
25913882	3	3	dep	achieved	767:774	arg1	L					796:796	1.43*10(7) cells m L	777:796	1.43*10(7) cells m L(-1)	777:800	Semi-continuous culture was found to be the most feasible strategy to develop microalgal biomass production facilities in higher latitudes, due to their average results in terms of growth rate (0.27 day(-1)), duplication time (2.5-4 days), maximum cell density achieved (1.43*10(7) cells m L(-1)), biomass productivity of 47.04 mg L(-1) day(-1) and an exopolysaccharides production of 2.1 g L(-1).
25913882	3	3	dep	achieved	767:774	arg1	-1					798:799	-1	798:799	-1	798:799	Semi-continuous culture was found to be the most feasible strategy to develop microalgal biomass production facilities in higher latitudes, due to their average results in terms of growth rate (0.27 day(-1)), duplication time (2.5-4 days), maximum cell density achieved (1.43*10(7) cells m L(-1)), biomass productivity of 47.04 mg L(-1) day(-1) and an exopolysaccharides production of 2.1 g L(-1).
25913882	3	3	dep	achieved	767:774	arg1	density					759:765	maximum cell density	746:765	maximum cell density achieved (1.43*10(7) cells m L(-1))	746:801	Semi-continuous culture was found to be the most feasible strategy to develop microalgal biomass production facilities in higher latitudes, due to their average results in terms of growth rate (0.27 day(-1)), duplication time (2.5-4 days), maximum cell density achieved (1.43*10(7) cells m L(-1)), biomass productivity of 47.04 mg L(-1) day(-1) and an exopolysaccharides production of 2.1 g L(-1).
25913882	0	4	theme	high	98:101	arg1	latitudes					103:111	high latitudes	98:111	high latitudes	98:111	Evaluation of batch and semi-continuous culture of Porphyridium purpureum in a photobioreactor in high latitudes using Fourier Transform Infrared spectroscopy for monitoring biomass composition and metabolites production.
25913882	0	5	from	Evaluation	0:9	arg1	photobioreactor					79:93	a photobioreactor	77:93	a photobioreactor in high latitudes	77:111	Evaluation of batch and semi-continuous culture of Porphyridium purpureum in a photobioreactor in high latitudes using Fourier Transform Infrared spectroscopy for monitoring biomass composition and metabolites production.
25913882	3	6	theme	g	895:895	arg1	-1					899:900	-1	899:900	-1	899:900	Semi-continuous culture was found to be the most feasible strategy to develop microalgal biomass production facilities in higher latitudes, due to their average results in terms of growth rate (0.27 day(-1)), duplication time (2.5-4 days), maximum cell density achieved (1.43*10(7) cells m L(-1)), biomass productivity of 47.04 mg L(-1) day(-1) and an exopolysaccharides production of 2.1 g L(-1).
25913882	3	6	theme	g	895:895	arg1	L					897:897	2.1 g L	891:897	2.1 g L(-1)	891:901	Semi-continuous culture was found to be the most feasible strategy to develop microalgal biomass production facilities in higher latitudes, due to their average results in terms of growth rate (0.27 day(-1)), duplication time (2.5-4 days), maximum cell density achieved (1.43*10(7) cells m L(-1)), biomass productivity of 47.04 mg L(-1) day(-1) and an exopolysaccharides production of 2.1 g L(-1).
25913882	3	7	theme	duplication	715:725	arg1	days					739:742	2.5-4 days	733:742	2.5-4 days	733:742	Semi-continuous culture was found to be the most feasible strategy to develop microalgal biomass production facilities in higher latitudes, due to their average results in terms of growth rate (0.27 day(-1)), duplication time (2.5-4 days), maximum cell density achieved (1.43*10(7) cells m L(-1)), biomass productivity of 47.04 mg L(-1) day(-1) and an exopolysaccharides production of 2.1 g L(-1).
25913882	3	7	theme	duplication	715:725	arg1	time					727:730	duplication time	715:730	duplication time (2.5-4 days)	715:743	Semi-continuous culture was found to be the most feasible strategy to develop microalgal biomass production facilities in higher latitudes, due to their average results in terms of growth rate (0.27 day(-1)), duplication time (2.5-4 days), maximum cell density achieved (1.43*10(7) cells m L(-1)), biomass productivity of 47.04 mg L(-1) day(-1) and an exopolysaccharides production of 2.1 g L(-1).
25913882	4	8	from	phycoerythrin	1175:1187	arg1	cultures					1227:1234	P. purpureum cultures	1214:1234	P. purpureum cultures	1214:1234	FTIR technology applied to microalgal production is a valuable and reliable tool to determine on a daily basis not just the evolution of macromolecules composition (lipids, carbohydrates and proteins) but also for the characterisation of the metabolites produced such as phycoerythrin or exopolysaccharides in P. purpureum cultures.
25913882	4	9	theme	reliable	971:978	arg1	technology					909:918	FTIR technology	904:918	FTIR technology applied to microalgal production	904:951	FTIR technology applied to microalgal production is a valuable and reliable tool to determine on a daily basis not just the evolution of macromolecules composition (lipids, carbohydrates and proteins) but also for the characterisation of the metabolites produced such as phycoerythrin or exopolysaccharides in P. purpureum cultures.
25913882	4	9	theme	reliable	971:978	arg1	tool					980:983	a valuable and reliable tool	956:983	a valuable and reliable tool to determine on a daily basis not just the evolution of macromolecules composition (lipids, carbohydrates and proteins) but also for the characterisation of the metabolites produced such as phycoerythrin or exopolysaccharides in P. purpureum cultures	956:1234	FTIR technology applied to microalgal production is a valuable and reliable tool to determine on a daily basis not just the evolution of macromolecules composition (lipids, carbohydrates and proteins) but also for the characterisation of the metabolites produced such as phycoerythrin or exopolysaccharides in P. purpureum cultures.
25913882	1	10	theme	biomass	288:294	arg1	formation					311:319	biomass and metabolite formation	288:319	formation	311:319	The culture strategy (batch or semi-continuous) was evaluated for biomass and metabolite formation in Porphyridium purpureum cultures in higher latitudes (>50° N).
25913882	4	11	theme	metabolites	1146:1156	arg1	characterisation					1122:1137	the characterisation	1118:1137	the characterisation of the metabolites produced such as phycoerythrin or exopolysaccharides in P. purpureum cultures	1118:1234	FTIR technology applied to microalgal production is a valuable and reliable tool to determine on a daily basis not just the evolution of macromolecules composition (lipids, carbohydrates and proteins) but also for the characterisation of the metabolites produced such as phycoerythrin or exopolysaccharides in P. purpureum cultures.
25913882	1	12	from	formation	311:319	arg1	cultures					347:354	Porphyridium purpureum cultures	324:354	Porphyridium purpureum cultures	324:354	The culture strategy (batch or semi-continuous) was evaluated for biomass and metabolite formation in Porphyridium purpureum cultures in higher latitudes (>50° N).
25913882	2	13	theme	biomass	441:447	arg1	composition					449:459	macromolecule biomass composition	427:459	macromolecule biomass composition	427:459	FTIR was used technology to characterise macromolecule biomass composition and the quality of the metabolites produced.
25913882	3	14	theme	feasible	555:562	arg1	strategy					564:571	the most feasible strategy to develop microalgal biomass production facilities in higher latitudes, due to their average results in terms of growth rate (0.27 day(-1)), duplication time (2.5-4 days), maximum cell density achieved (1.43*10(7) cells m L(-1)), biomass productivity of 47.04 mg L(-1) day(-1) and an exopolysaccharides production of 2.1 g L(-1)	546:901	the most feasible strategy to develop microalgal biomass production facilities in higher latitudes, due to their average results in terms of growth rate (0.27 day(-1)), duplication time (2.5-4 days), maximum cell density achieved (1.43*10(7) cells m L(-1)), biomass productivity of 47.04 mg L(-1) day(-1) and an exopolysaccharides production of 2.1 g L(-1)	546:901	Semi-continuous culture was found to be the most feasible strategy to develop microalgal biomass production facilities in higher latitudes, due to their average results in terms of growth rate (0.27 day(-1)), duplication time (2.5-4 days), maximum cell density achieved (1.43*10(7) cells m L(-1)), biomass productivity of 47.04 mg L(-1) day(-1) and an exopolysaccharides production of 2.1 g L(-1).
25913882	3	14	theme	feasible	555:562	arg1	culture					522:528	Semi-continuous culture	506:528	Semi-continuous culture	506:528	Semi-continuous culture was found to be the most feasible strategy to develop microalgal biomass production facilities in higher latitudes, due to their average results in terms of growth rate (0.27 day(-1)), duplication time (2.5-4 days), maximum cell density achieved (1.43*10(7) cells m L(-1)), biomass productivity of 47.04 mg L(-1) day(-1) and an exopolysaccharides production of 2.1 g L(-1).
25913882	4	15	dep	basis	1009:1013	arg1	just					1019:1022	just	1019:1022	just	1019:1022	FTIR technology applied to microalgal production is a valuable and reliable tool to determine on a daily basis not just the evolution of macromolecules composition (lipids, carbohydrates and proteins) but also for the characterisation of the metabolites produced such as phycoerythrin or exopolysaccharides in P. purpureum cultures.
25913882	3	16	theme	exopolysaccharides	858:875	arg1	production					877:886	an exopolysaccharides production	855:886	an exopolysaccharides production of 2.1 g L(-1)	855:901	Semi-continuous culture was found to be the most feasible strategy to develop microalgal biomass production facilities in higher latitudes, due to their average results in terms of growth rate (0.27 day(-1)), duplication time (2.5-4 days), maximum cell density achieved (1.43*10(7) cells m L(-1)), biomass productivity of 47.04 mg L(-1) day(-1) and an exopolysaccharides production of 2.1 g L(-1).
25913882	1	17	dep	Porphyridium	324:335	arg1	purpureum					337:345	purpureum	337:345	purpureum	337:345	The culture strategy (batch or semi-continuous) was evaluated for biomass and metabolite formation in Porphyridium purpureum cultures in higher latitudes (>50° N).
25913882	4	18	theme	composition	1056:1066	arg1	evolution					1028:1036	the evolution	1024:1036	a daily basis not just the evolution of macromolecules composition (lipids, carbohydrates and proteins)	1001:1103	FTIR technology applied to microalgal production is a valuable and reliable tool to determine on a daily basis not just the evolution of macromolecules composition (lipids, carbohydrates and proteins) but also for the characterisation of the metabolites produced such as phycoerythrin or exopolysaccharides in P. purpureum cultures.
25913882	3	19	theme	biomass	804:810	arg1	productivity					812:823	biomass productivity	804:823	biomass productivity of 47.04 mg L(-1) day(-1)	804:849	Semi-continuous culture was found to be the most feasible strategy to develop microalgal biomass production facilities in higher latitudes, due to their average results in terms of growth rate (0.27 day(-1)), duplication time (2.5-4 days), maximum cell density achieved (1.43*10(7) cells m L(-1)), biomass productivity of 47.04 mg L(-1) day(-1) and an exopolysaccharides production of 2.1 g L(-1).
25913882	4	20	theme	macromolecules	1041:1054	arg1	composition					1056:1066	macromolecules composition	1041:1066	macromolecules composition (lipids, carbohydrates and proteins)	1041:1103	FTIR technology applied to microalgal production is a valuable and reliable tool to determine on a daily basis not just the evolution of macromolecules composition (lipids, carbohydrates and proteins) but also for the characterisation of the metabolites produced such as phycoerythrin or exopolysaccharides in P. purpureum cultures.
25913882	3	21	theme	production	603:612	arg1	facilities					614:623	microalgal biomass production facilities	584:623	microalgal biomass production facilities in higher latitudes	584:643	Semi-continuous culture was found to be the most feasible strategy to develop microalgal biomass production facilities in higher latitudes, due to their average results in terms of growth rate (0.27 day(-1)), duplication time (2.5-4 days), maximum cell density achieved (1.43*10(7) cells m L(-1)), biomass productivity of 47.04 mg L(-1) day(-1) and an exopolysaccharides production of 2.1 g L(-1).
25913882	3	22	from	facilities	614:623	arg1	latitudes					635:643	higher latitudes	628:643	higher latitudes	628:643	Semi-continuous culture was found to be the most feasible strategy to develop microalgal biomass production facilities in higher latitudes, due to their average results in terms of growth rate (0.27 day(-1)), duplication time (2.5-4 days), maximum cell density achieved (1.43*10(7) cells m L(-1)), biomass productivity of 47.04 mg L(-1) day(-1) and an exopolysaccharides production of 2.1 g L(-1).
25913882	3	23	theme	L	897:897	arg1	time					727:730	duplication time	715:730	duplication time (2.5-4 days)	715:743	Semi-continuous culture was found to be the most feasible strategy to develop microalgal biomass production facilities in higher latitudes, due to their average results in terms of growth rate (0.27 day(-1)), duplication time (2.5-4 days), maximum cell density achieved (1.43*10(7) cells m L(-1)), biomass productivity of 47.04 mg L(-1) day(-1) and an exopolysaccharides production of 2.1 g L(-1).
25913882	3	23	theme	L	897:897	arg1	L					796:796	1.43*10(7) cells m L	777:796	1.43*10(7) cells m L(-1)	777:800	Semi-continuous culture was found to be the most feasible strategy to develop microalgal biomass production facilities in higher latitudes, due to their average results in terms of growth rate (0.27 day(-1)), duplication time (2.5-4 days), maximum cell density achieved (1.43*10(7) cells m L(-1)), biomass productivity of 47.04 mg L(-1) day(-1) and an exopolysaccharides production of 2.1 g L(-1).
25913882	3	23	theme	L	897:897	arg1	productivity					812:823	biomass productivity	804:823	biomass productivity of 47.04 mg L(-1) day(-1)	804:849	Semi-continuous culture was found to be the most feasible strategy to develop microalgal biomass production facilities in higher latitudes, due to their average results in terms of growth rate (0.27 day(-1)), duplication time (2.5-4 days), maximum cell density achieved (1.43*10(7) cells m L(-1)), biomass productivity of 47.04 mg L(-1) day(-1) and an exopolysaccharides production of 2.1 g L(-1).
25913882	3	23	theme	L	897:897	arg1	density					759:765	maximum cell density	746:765	maximum cell density achieved (1.43*10(7) cells m L(-1))	746:801	Semi-continuous culture was found to be the most feasible strategy to develop microalgal biomass production facilities in higher latitudes, due to their average results in terms of growth rate (0.27 day(-1)), duplication time (2.5-4 days), maximum cell density achieved (1.43*10(7) cells m L(-1)), biomass productivity of 47.04 mg L(-1) day(-1) and an exopolysaccharides production of 2.1 g L(-1).
25913882	3	23	theme	L	897:897	arg1	days					739:742	2.5-4 days	733:742	2.5-4 days	733:742	Semi-continuous culture was found to be the most feasible strategy to develop microalgal biomass production facilities in higher latitudes, due to their average results in terms of growth rate (0.27 day(-1)), duplication time (2.5-4 days), maximum cell density achieved (1.43*10(7) cells m L(-1)), biomass productivity of 47.04 mg L(-1) day(-1) and an exopolysaccharides production of 2.1 g L(-1).
25913882	3	23	theme	L	897:897	arg1	production					877:886	an exopolysaccharides production	855:886	an exopolysaccharides production of 2.1 g L(-1)	855:901	Semi-continuous culture was found to be the most feasible strategy to develop microalgal biomass production facilities in higher latitudes, due to their average results in terms of growth rate (0.27 day(-1)), duplication time (2.5-4 days), maximum cell density achieved (1.43*10(7) cells m L(-1)), biomass productivity of 47.04 mg L(-1) day(-1) and an exopolysaccharides production of 2.1 g L(-1).
25913882	4	24	theme	purpureum	1217:1225	arg1	cultures					1227:1234	P. purpureum cultures	1214:1234	P. purpureum cultures	1214:1234	FTIR technology applied to microalgal production is a valuable and reliable tool to determine on a daily basis not just the evolution of macromolecules composition (lipids, carbohydrates and proteins) but also for the characterisation of the metabolites produced such as phycoerythrin or exopolysaccharides in P. purpureum cultures.
25913882	4	25	theme	valuable	958:965	arg1	technology					909:918	FTIR technology	904:918	FTIR technology applied to microalgal production	904:951	FTIR technology applied to microalgal production is a valuable and reliable tool to determine on a daily basis not just the evolution of macromolecules composition (lipids, carbohydrates and proteins) but also for the characterisation of the metabolites produced such as phycoerythrin or exopolysaccharides in P. purpureum cultures.
25913882	4	25	theme	valuable	958:965	arg1	tool					980:983	a valuable and reliable tool	956:983	a valuable and reliable tool to determine on a daily basis not just the evolution of macromolecules composition (lipids, carbohydrates and proteins) but also for the characterisation of the metabolites produced such as phycoerythrin or exopolysaccharides in P. purpureum cultures	956:1234	FTIR technology applied to microalgal production is a valuable and reliable tool to determine on a daily basis not just the evolution of macromolecules composition (lipids, carbohydrates and proteins) but also for the characterisation of the metabolites produced such as phycoerythrin or exopolysaccharides in P. purpureum cultures.
25913882	1	26	theme	metabolite	300:309	arg1	formation					311:319	biomass and metabolite formation	288:319	formation	311:319	The culture strategy (batch or semi-continuous) was evaluated for biomass and metabolite formation in Porphyridium purpureum cultures in higher latitudes (>50° N).
25913882	3	27	theme	microalgal	584:593	arg1	facilities					614:623	microalgal biomass production facilities	584:623	microalgal biomass production facilities in higher latitudes	584:643	Semi-continuous culture was found to be the most feasible strategy to develop microalgal biomass production facilities in higher latitudes, due to their average results in terms of growth rate (0.27 day(-1)), duplication time (2.5-4 days), maximum cell density achieved (1.43*10(7) cells m L(-1)), biomass productivity of 47.04 mg L(-1) day(-1) and an exopolysaccharides production of 2.1 g L(-1).
25913882	0	28	theme	batch	14:18	arg1	culture					40:46	batch and semi-continuous culture	14:46	batch and semi-continuous culture of Porphyridium purpureum in a photobioreactor in high latitudes	14:111	Evaluation of batch and semi-continuous culture of Porphyridium purpureum in a photobioreactor in high latitudes using Fourier Transform Infrared spectroscopy for monitoring biomass composition and metabolites production.
25913882	0	29	from	photobioreactor	79:93	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of batch and semi-continuous culture of Porphyridium purpureum in a photobioreactor in high latitudes	0:111	Evaluation of batch and semi-continuous culture of Porphyridium purpureum in a photobioreactor in high latitudes using Fourier Transform Infrared spectroscopy for monitoring biomass composition and metabolites production.
25913882	0	29	from	photobioreactor	79:93	arg1	latitudes					103:111	high latitudes	98:111	high latitudes	98:111	Evaluation of batch and semi-continuous culture of Porphyridium purpureum in a photobioreactor in high latitudes using Fourier Transform Infrared spectroscopy for monitoring biomass composition and metabolites production.
25913882	3	30	theme	biomass	595:601	arg1	facilities					614:623	microalgal biomass production facilities	584:623	microalgal biomass production facilities in higher latitudes	584:643	Semi-continuous culture was found to be the most feasible strategy to develop microalgal biomass production facilities in higher latitudes, due to their average results in terms of growth rate (0.27 day(-1)), duplication time (2.5-4 days), maximum cell density achieved (1.43*10(7) cells m L(-1)), biomass productivity of 47.04 mg L(-1) day(-1) and an exopolysaccharides production of 2.1 g L(-1).
25913882	3	31	theme	mg	834:835	arg1	-1					847:848	-1	847:848	-1	847:848	Semi-continuous culture was found to be the most feasible strategy to develop microalgal biomass production facilities in higher latitudes, due to their average results in terms of growth rate (0.27 day(-1)), duplication time (2.5-4 days), maximum cell density achieved (1.43*10(7) cells m L(-1)), biomass productivity of 47.04 mg L(-1) day(-1) and an exopolysaccharides production of 2.1 g L(-1).
25913882	3	31	theme	mg	834:835	arg1	day					843:845	47.04 mg L(-1) day	828:845	47.04 mg L(-1) day(-1)	828:849	Semi-continuous culture was found to be the most feasible strategy to develop microalgal biomass production facilities in higher latitudes, due to their average results in terms of growth rate (0.27 day(-1)), duplication time (2.5-4 days), maximum cell density achieved (1.43*10(7) cells m L(-1)), biomass productivity of 47.04 mg L(-1) day(-1) and an exopolysaccharides production of 2.1 g L(-1).
25913882	1	32	theme	culture	226:232	arg1	strategy					234:241	The culture strategy	222:241	The culture strategy (batch or semi-continuous)	222:268	The culture strategy (batch or semi-continuous) was evaluated for biomass and metabolite formation in Porphyridium purpureum cultures in higher latitudes (>50° N).
25913882	0	33	theme	culture	40:46	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of batch and semi-continuous culture of Porphyridium purpureum in a photobioreactor in high latitudes	0:111	Evaluation of batch and semi-continuous culture of Porphyridium purpureum in a photobioreactor in high latitudes using Fourier Transform Infrared spectroscopy for monitoring biomass composition and metabolites production.
25913882	3	34	theme	L	837:837	arg1	-1					847:848	-1	847:848	-1	847:848	Semi-continuous culture was found to be the most feasible strategy to develop microalgal biomass production facilities in higher latitudes, due to their average results in terms of growth rate (0.27 day(-1)), duplication time (2.5-4 days), maximum cell density achieved (1.43*10(7) cells m L(-1)), biomass productivity of 47.04 mg L(-1) day(-1) and an exopolysaccharides production of 2.1 g L(-1).
25913882	3	34	theme	L	837:837	arg1	day					843:845	47.04 mg L(-1) day	828:845	47.04 mg L(-1) day(-1)	828:849	Semi-continuous culture was found to be the most feasible strategy to develop microalgal biomass production facilities in higher latitudes, due to their average results in terms of growth rate (0.27 day(-1)), duplication time (2.5-4 days), maximum cell density achieved (1.43*10(7) cells m L(-1)), biomass productivity of 47.04 mg L(-1) day(-1) and an exopolysaccharides production of 2.1 g L(-1).
25913882	2	35	theme	macromolecule	427:439	arg1	composition					449:459	macromolecule biomass composition	427:459	macromolecule biomass composition	427:459	FTIR was used technology to characterise macromolecule biomass composition and the quality of the metabolites produced.
25913882	0	36	theme	semi-continuous	24:38	arg1	culture					40:46	batch and semi-continuous culture	14:46	batch and semi-continuous culture of Porphyridium purpureum in a photobioreactor in high latitudes	14:111	Evaluation of batch and semi-continuous culture of Porphyridium purpureum in a photobioreactor in high latitudes using Fourier Transform Infrared spectroscopy for monitoring biomass composition and metabolites production.
25913882	1	37	theme	Porphyridium	324:335	arg1	cultures					347:354	Porphyridium purpureum cultures	324:354	Porphyridium purpureum cultures	324:354	The culture strategy (batch or semi-continuous) was evaluated for biomass and metabolite formation in Porphyridium purpureum cultures in higher latitudes (>50° N).
25913882	0	38	theme	Infrared	137:144	arg1	spectroscopy					146:157	Infrared spectroscopy	137:157	Infrared spectroscopy	137:157	Evaluation of batch and semi-continuous culture of Porphyridium purpureum in a photobioreactor in high latitudes using Fourier Transform Infrared spectroscopy for monitoring biomass composition and metabolites production.
25913882	2	39	theme	metabolites	484:494	arg1	quality					469:475	the quality	465:475	the quality of the metabolites produced	465:503	FTIR was used technology to characterise macromolecule biomass composition and the quality of the metabolites produced.
25913882	2	39	theme	metabolites	484:494	arg1	composition					449:459	macromolecule biomass composition	427:459	macromolecule biomass composition	427:459	FTIR was used technology to characterise macromolecule biomass composition and the quality of the metabolites produced.
25913882	3	40	theme	cell	754:757	arg1	L					796:796	1.43*10(7) cells m L	777:796	1.43*10(7) cells m L(-1)	777:800	Semi-continuous culture was found to be the most feasible strategy to develop microalgal biomass production facilities in higher latitudes, due to their average results in terms of growth rate (0.27 day(-1)), duplication time (2.5-4 days), maximum cell density achieved (1.43*10(7) cells m L(-1)), biomass productivity of 47.04 mg L(-1) day(-1) and an exopolysaccharides production of 2.1 g L(-1).
25913882	3	40	theme	cell	754:757	arg1	density					759:765	maximum cell density	746:765	maximum cell density achieved (1.43*10(7) cells m L(-1))	746:801	Semi-continuous culture was found to be the most feasible strategy to develop microalgal biomass production facilities in higher latitudes, due to their average results in terms of growth rate (0.27 day(-1)), duplication time (2.5-4 days), maximum cell density achieved (1.43*10(7) cells m L(-1)), biomass productivity of 47.04 mg L(-1) day(-1) and an exopolysaccharides production of 2.1 g L(-1).
25913882	0	41	from	culture	40:46	arg1	photobioreactor					79:93	a photobioreactor	77:93	a photobioreactor in high latitudes	77:111	Evaluation of batch and semi-continuous culture of Porphyridium purpureum in a photobioreactor in high latitudes using Fourier Transform Infrared spectroscopy for monitoring biomass composition and metabolites production.
25913882	3	42	theme	higher	628:633	arg1	latitudes					635:643	higher latitudes	628:643	higher latitudes	628:643	Semi-continuous culture was found to be the most feasible strategy to develop microalgal biomass production facilities in higher latitudes, due to their average results in terms of growth rate (0.27 day(-1)), duplication time (2.5-4 days), maximum cell density achieved (1.43*10(7) cells m L(-1)), biomass productivity of 47.04 mg L(-1) day(-1) and an exopolysaccharides production of 2.1 g L(-1).
25913882	3	43	from	results	667:673	arg1	terms					678:682	terms	678:682	terms of growth rate (0.27 day(-1))	678:712	Semi-continuous culture was found to be the most feasible strategy to develop microalgal biomass production facilities in higher latitudes, due to their average results in terms of growth rate (0.27 day(-1)), duplication time (2.5-4 days), maximum cell density achieved (1.43*10(7) cells m L(-1)), biomass productivity of 47.04 mg L(-1) day(-1) and an exopolysaccharides production of 2.1 g L(-1).
25913882	3	44	theme	maximum	746:752	arg1	L					796:796	1.43*10(7) cells m L	777:796	1.43*10(7) cells m L(-1)	777:800	Semi-continuous culture was found to be the most feasible strategy to develop microalgal biomass production facilities in higher latitudes, due to their average results in terms of growth rate (0.27 day(-1)), duplication time (2.5-4 days), maximum cell density achieved (1.43*10(7) cells m L(-1)), biomass productivity of 47.04 mg L(-1) day(-1) and an exopolysaccharides production of 2.1 g L(-1).
25913882	3	44	theme	maximum	746:752	arg1	density					759:765	maximum cell density	746:765	maximum cell density achieved (1.43*10(7) cells m L(-1))	746:801	Semi-continuous culture was found to be the most feasible strategy to develop microalgal biomass production facilities in higher latitudes, due to their average results in terms of growth rate (0.27 day(-1)), duplication time (2.5-4 days), maximum cell density achieved (1.43*10(7) cells m L(-1)), biomass productivity of 47.04 mg L(-1) day(-1) and an exopolysaccharides production of 2.1 g L(-1).
25913882	0	45	theme	composition	182:192	arg1	production					210:219	biomass composition and metabolites production	174:219	biomass composition and metabolites production	174:219	Evaluation of batch and semi-continuous culture of Porphyridium purpureum in a photobioreactor in high latitudes using Fourier Transform Infrared spectroscopy for monitoring biomass composition and metabolites production.
25913882	4	46	dep	composition	1056:1066	arg1	carbohydrates					1077:1089	carbohydrates	1077:1089	carbohydrates	1077:1089	FTIR technology applied to microalgal production is a valuable and reliable tool to determine on a daily basis not just the evolution of macromolecules composition (lipids, carbohydrates and proteins) but also for the characterisation of the metabolites produced such as phycoerythrin or exopolysaccharides in P. purpureum cultures.
25913882	4	46	dep	composition	1056:1066	arg1	lipids					1069:1074	lipids	1069:1074	lipids	1069:1074	FTIR technology applied to microalgal production is a valuable and reliable tool to determine on a daily basis not just the evolution of macromolecules composition (lipids, carbohydrates and proteins) but also for the characterisation of the metabolites produced such as phycoerythrin or exopolysaccharides in P. purpureum cultures.
25913882	4	46	dep	composition	1056:1066	arg1	proteins					1095:1102	proteins	1095:1102	proteins	1095:1102	FTIR technology applied to microalgal production is a valuable and reliable tool to determine on a daily basis not just the evolution of macromolecules composition (lipids, carbohydrates and proteins) but also for the characterisation of the metabolites produced such as phycoerythrin or exopolysaccharides in P. purpureum cultures.
25913882	1	47	dep	strategy	234:241	arg1	semi-continuous					253:267	semi-continuous	253:267	semi-continuous	253:267	The culture strategy (batch or semi-continuous) was evaluated for biomass and metabolite formation in Porphyridium purpureum cultures in higher latitudes (>50° N).
25913882	1	47	dep	strategy	234:241	arg1	batch					244:248	batch	244:248	batch	244:248	The culture strategy (batch or semi-continuous) was evaluated for biomass and metabolite formation in Porphyridium purpureum cultures in higher latitudes (>50° N).
25913882	3	48	theme	day	843:845	arg1	time					727:730	duplication time	715:730	duplication time (2.5-4 days)	715:743	Semi-continuous culture was found to be the most feasible strategy to develop microalgal biomass production facilities in higher latitudes, due to their average results in terms of growth rate (0.27 day(-1)), duplication time (2.5-4 days), maximum cell density achieved (1.43*10(7) cells m L(-1)), biomass productivity of 47.04 mg L(-1) day(-1) and an exopolysaccharides production of 2.1 g L(-1).
25913882	3	48	theme	day	843:845	arg1	L					796:796	1.43*10(7) cells m L	777:796	1.43*10(7) cells m L(-1)	777:800	Semi-continuous culture was found to be the most feasible strategy to develop microalgal biomass production facilities in higher latitudes, due to their average results in terms of growth rate (0.27 day(-1)), duplication time (2.5-4 days), maximum cell density achieved (1.43*10(7) cells m L(-1)), biomass productivity of 47.04 mg L(-1) day(-1) and an exopolysaccharides production of 2.1 g L(-1).
25913882	3	48	theme	day	843:845	arg1	productivity					812:823	biomass productivity	804:823	biomass productivity of 47.04 mg L(-1) day(-1)	804:849	Semi-continuous culture was found to be the most feasible strategy to develop microalgal biomass production facilities in higher latitudes, due to their average results in terms of growth rate (0.27 day(-1)), duplication time (2.5-4 days), maximum cell density achieved (1.43*10(7) cells m L(-1)), biomass productivity of 47.04 mg L(-1) day(-1) and an exopolysaccharides production of 2.1 g L(-1).
25913882	3	48	theme	day	843:845	arg1	density					759:765	maximum cell density	746:765	maximum cell density achieved (1.43*10(7) cells m L(-1))	746:801	Semi-continuous culture was found to be the most feasible strategy to develop microalgal biomass production facilities in higher latitudes, due to their average results in terms of growth rate (0.27 day(-1)), duplication time (2.5-4 days), maximum cell density achieved (1.43*10(7) cells m L(-1)), biomass productivity of 47.04 mg L(-1) day(-1) and an exopolysaccharides production of 2.1 g L(-1).
25913882	3	48	theme	day	843:845	arg1	days					739:742	2.5-4 days	733:742	2.5-4 days	733:742	Semi-continuous culture was found to be the most feasible strategy to develop microalgal biomass production facilities in higher latitudes, due to their average results in terms of growth rate (0.27 day(-1)), duplication time (2.5-4 days), maximum cell density achieved (1.43*10(7) cells m L(-1)), biomass productivity of 47.04 mg L(-1) day(-1) and an exopolysaccharides production of 2.1 g L(-1).
25913882	3	48	theme	day	843:845	arg1	production					877:886	an exopolysaccharides production	855:886	an exopolysaccharides production of 2.1 g L(-1)	855:901	Semi-continuous culture was found to be the most feasible strategy to develop microalgal biomass production facilities in higher latitudes, due to their average results in terms of growth rate (0.27 day(-1)), duplication time (2.5-4 days), maximum cell density achieved (1.43*10(7) cells m L(-1)), biomass productivity of 47.04 mg L(-1) day(-1) and an exopolysaccharides production of 2.1 g L(-1).
25913882	3	49	theme	Semi-continuous	506:520	arg1	culture					522:528	Semi-continuous culture	506:528	Semi-continuous culture	506:528	Semi-continuous culture was found to be the most feasible strategy to develop microalgal biomass production facilities in higher latitudes, due to their average results in terms of growth rate (0.27 day(-1)), duplication time (2.5-4 days), maximum cell density achieved (1.43*10(7) cells m L(-1)), biomass productivity of 47.04 mg L(-1) day(-1) and an exopolysaccharides production of 2.1 g L(-1).
25913882	3	49	theme	Semi-continuous	506:520	arg1	strategy					564:571	the most feasible strategy to develop microalgal biomass production facilities in higher latitudes, due to their average results in terms of growth rate (0.27 day(-1)), duplication time (2.5-4 days), maximum cell density achieved (1.43*10(7) cells m L(-1)), biomass productivity of 47.04 mg L(-1) day(-1) and an exopolysaccharides production of 2.1 g L(-1)	546:901	the most feasible strategy to develop microalgal biomass production facilities in higher latitudes, due to their average results in terms of growth rate (0.27 day(-1)), duplication time (2.5-4 days), maximum cell density achieved (1.43*10(7) cells m L(-1)), biomass productivity of 47.04 mg L(-1) day(-1) and an exopolysaccharides production of 2.1 g L(-1)	546:901	Semi-continuous culture was found to be the most feasible strategy to develop microalgal biomass production facilities in higher latitudes, due to their average results in terms of growth rate (0.27 day(-1)), duplication time (2.5-4 days), maximum cell density achieved (1.43*10(7) cells m L(-1)), biomass productivity of 47.04 mg L(-1) day(-1) and an exopolysaccharides production of 2.1 g L(-1).
25913882	4	50	theme	microalgal	931:940	arg1	production					942:951	microalgal production	931:951	microalgal production	931:951	FTIR technology applied to microalgal production is a valuable and reliable tool to determine on a daily basis not just the evolution of macromolecules composition (lipids, carbohydrates and proteins) but also for the characterisation of the metabolites produced such as phycoerythrin or exopolysaccharides in P. purpureum cultures.
25913882	0	51	theme	biomass	174:180	arg1	composition					182:192	biomass composition	174:192	biomass composition	174:192	Evaluation of batch and semi-continuous culture of Porphyridium purpureum in a photobioreactor in high latitudes using Fourier Transform Infrared spectroscopy for monitoring biomass composition and metabolites production.
25913882	3	52	theme	growth	687:692	arg1	rate					694:697	growth rate	687:697	growth rate (0.27 day(-1))	687:712	Semi-continuous culture was found to be the most feasible strategy to develop microalgal biomass production facilities in higher latitudes, due to their average results in terms of growth rate (0.27 day(-1)), duplication time (2.5-4 days), maximum cell density achieved (1.43*10(7) cells m L(-1)), biomass productivity of 47.04 mg L(-1) day(-1) and an exopolysaccharides production of 2.1 g L(-1).
25913882	3	52	theme	growth	687:692	arg1	day					705:707	0.27 day(-1)	700:711	0.27 day(-1)	700:711	Semi-continuous culture was found to be the most feasible strategy to develop microalgal biomass production facilities in higher latitudes, due to their average results in terms of growth rate (0.27 day(-1)), duplication time (2.5-4 days), maximum cell density achieved (1.43*10(7) cells m L(-1)), biomass productivity of 47.04 mg L(-1) day(-1) and an exopolysaccharides production of 2.1 g L(-1).
25913882	3	53	theme	average	659:665	arg1	results					667:673	their average results	653:673	their average results in terms of growth rate (0.27 day(-1))	653:712	Semi-continuous culture was found to be the most feasible strategy to develop microalgal biomass production facilities in higher latitudes, due to their average results in terms of growth rate (0.27 day(-1)), duplication time (2.5-4 days), maximum cell density achieved (1.43*10(7) cells m L(-1)), biomass productivity of 47.04 mg L(-1) day(-1) and an exopolysaccharides production of 2.1 g L(-1).
25913882	4	54	theme	daily	1003:1007	arg1	basis					1009:1013	a daily basis	1001:1013	a daily basis not just the evolution of macromolecules composition (lipids, carbohydrates and proteins)	1001:1103	FTIR technology applied to microalgal production is a valuable and reliable tool to determine on a daily basis not just the evolution of macromolecules composition (lipids, carbohydrates and proteins) but also for the characterisation of the metabolites produced such as phycoerythrin or exopolysaccharides in P. purpureum cultures.
25913882	3	55	theme	cells	788:792	arg1	L					796:796	1.43*10(7) cells m L	777:796	1.43*10(7) cells m L(-1)	777:800	Semi-continuous culture was found to be the most feasible strategy to develop microalgal biomass production facilities in higher latitudes, due to their average results in terms of growth rate (0.27 day(-1)), duplication time (2.5-4 days), maximum cell density achieved (1.43*10(7) cells m L(-1)), biomass productivity of 47.04 mg L(-1) day(-1) and an exopolysaccharides production of 2.1 g L(-1).
25913882	3	55	theme	cells	788:792	arg1	-1					798:799	-1	798:799	-1	798:799	Semi-continuous culture was found to be the most feasible strategy to develop microalgal biomass production facilities in higher latitudes, due to their average results in terms of growth rate (0.27 day(-1)), duplication time (2.5-4 days), maximum cell density achieved (1.43*10(7) cells m L(-1)), biomass productivity of 47.04 mg L(-1) day(-1) and an exopolysaccharides production of 2.1 g L(-1).
25913882	3	55	theme	cells	788:792	arg1	density					759:765	maximum cell density	746:765	maximum cell density achieved (1.43*10(7) cells m L(-1))	746:801	Semi-continuous culture was found to be the most feasible strategy to develop microalgal biomass production facilities in higher latitudes, due to their average results in terms of growth rate (0.27 day(-1)), duplication time (2.5-4 days), maximum cell density achieved (1.43*10(7) cells m L(-1)), biomass productivity of 47.04 mg L(-1) day(-1) and an exopolysaccharides production of 2.1 g L(-1).
25913882	3	56	theme	*	781:781	arg1	cells					788:792	1.43*10(7) cells	777:792	1.43*10(7) cells m L(-1)	777:800	Semi-continuous culture was found to be the most feasible strategy to develop microalgal biomass production facilities in higher latitudes, due to their average results in terms of growth rate (0.27 day(-1)), duplication time (2.5-4 days), maximum cell density achieved (1.43*10(7) cells m L(-1)), biomass productivity of 47.04 mg L(-1) day(-1) and an exopolysaccharides production of 2.1 g L(-1).
25913882	3	57	theme	rate	694:697	arg1	terms					678:682	terms	678:682	terms of growth rate (0.27 day(-1))	678:712	Semi-continuous culture was found to be the most feasible strategy to develop microalgal biomass production facilities in higher latitudes, due to their average results in terms of growth rate (0.27 day(-1)), duplication time (2.5-4 days), maximum cell density achieved (1.43*10(7) cells m L(-1)), biomass productivity of 47.04 mg L(-1) day(-1) and an exopolysaccharides production of 2.1 g L(-1).
25913882	2	58	used	used	395:398	arg2	FTIR					386:389	FTIR	386:389	FTIR	386:389	FTIR was used technology to characterise macromolecule biomass composition and the quality of the metabolites produced.
25913882	3	59	theme	m	794:794	arg1	L					796:796	1.43*10(7) cells m L	777:796	1.43*10(7) cells m L(-1)	777:800	Semi-continuous culture was found to be the most feasible strategy to develop microalgal biomass production facilities in higher latitudes, due to their average results in terms of growth rate (0.27 day(-1)), duplication time (2.5-4 days), maximum cell density achieved (1.43*10(7) cells m L(-1)), biomass productivity of 47.04 mg L(-1) day(-1) and an exopolysaccharides production of 2.1 g L(-1).
25913882	3	59	theme	m	794:794	arg1	-1					798:799	-1	798:799	-1	798:799	Semi-continuous culture was found to be the most feasible strategy to develop microalgal biomass production facilities in higher latitudes, due to their average results in terms of growth rate (0.27 day(-1)), duplication time (2.5-4 days), maximum cell density achieved (1.43*10(7) cells m L(-1)), biomass productivity of 47.04 mg L(-1) day(-1) and an exopolysaccharides production of 2.1 g L(-1).
25913882	3	59	theme	m	794:794	arg1	density					759:765	maximum cell density	746:765	maximum cell density achieved (1.43*10(7) cells m L(-1))	746:801	Semi-continuous culture was found to be the most feasible strategy to develop microalgal biomass production facilities in higher latitudes, due to their average results in terms of growth rate (0.27 day(-1)), duplication time (2.5-4 days), maximum cell density achieved (1.43*10(7) cells m L(-1)), biomass productivity of 47.04 mg L(-1) day(-1) and an exopolysaccharides production of 2.1 g L(-1).
25913882	0	60	theme	purpureum	64:72	arg1	culture					40:46	batch and semi-continuous culture	14:46	batch and semi-continuous culture of Porphyridium purpureum in a photobioreactor in high latitudes	14:111	Evaluation of batch and semi-continuous culture of Porphyridium purpureum in a photobioreactor in high latitudes using Fourier Transform Infrared spectroscopy for monitoring biomass composition and metabolites production.
25913882	1	61	theme	higher	359:364	arg1	latitudes					366:374	higher latitudes	359:374	higher latitudes (>50° N)	359:383	The culture strategy (batch or semi-continuous) was evaluated for biomass and metabolite formation in Porphyridium purpureum cultures in higher latitudes (>50° N).
25913882	1	61	theme	higher	359:364	arg1	N					382:382	>50° N	377:382	>50° N	377:382	The culture strategy (batch or semi-continuous) was evaluated for biomass and metabolite formation in Porphyridium purpureum cultures in higher latitudes (>50° N).
25913882	4	62	theme	FTIR	904:907	arg1	technology					909:918	FTIR technology	904:918	FTIR technology applied to microalgal production	904:951	FTIR technology applied to microalgal production is a valuable and reliable tool to determine on a daily basis not just the evolution of macromolecules composition (lipids, carbohydrates and proteins) but also for the characterisation of the metabolites produced such as phycoerythrin or exopolysaccharides in P. purpureum cultures.
25913882	4	62	theme	FTIR	904:907	arg1	tool					980:983	a valuable and reliable tool	956:983	a valuable and reliable tool to determine on a daily basis not just the evolution of macromolecules composition (lipids, carbohydrates and proteins) but also for the characterisation of the metabolites produced such as phycoerythrin or exopolysaccharides in P. purpureum cultures	956:1234	FTIR technology applied to microalgal production is a valuable and reliable tool to determine on a daily basis not just the evolution of macromolecules composition (lipids, carbohydrates and proteins) but also for the characterisation of the metabolites produced such as phycoerythrin or exopolysaccharides in P. purpureum cultures.
25913882	4	63	theme	P.	1214:1215	arg1	cultures					1227:1234	P. purpureum cultures	1214:1234	P. purpureum cultures	1214:1234	FTIR technology applied to microalgal production is a valuable and reliable tool to determine on a daily basis not just the evolution of macromolecules composition (lipids, carbohydrates and proteins) but also for the characterisation of the metabolites produced such as phycoerythrin or exopolysaccharides in P. purpureum cultures.
25896518	4	0	theme	gut	865:867	arg1	species					879:885	gut bacterial species	865:885	gut bacterial species	865:885	We analyzed publicly available metagenomic data from fecal samples to characterize inter-individual variation in gut bacterial species.
25896518	3	1	from	gut	649:651	arg1	case					680:683	this case	675:683	this case	675:683	Unlike pan-genome studies of cultivable strains, the use of metagenomic data can provide an unbiased view of structural variation of gut bacterial strains by measuring them in their natural habitats, the gut of each individual in this case, representing native boundaries between gut bacterial populations.
25896518	6	2	theme	cultivable	1232:1241	arg1	species					1243:1249	cultivable species	1232:1249	cultivable species	1232:1249	This number is based on gene deletions only and represents a lower limit, yet the variation is already in a similar range as observed between completely sequenced strains of cultivable species.
25896518	2	3	theme	strains	353:359	arg1	presence					331:338	the presence	327:338	the presence of different strains of the same species	327:379	It is unclear whether gene content variation is caused by differences in microbial species composition or by the presence of different strains of the same species; the extent of gene content variation in the latter is unknown.
25896518	9	4	theme	variability	1642:1652	arg1	degree					1615:1620	The large degree	1605:1620	The large degree of inter-individual variability in gene content	1605:1668	The large degree of inter-individual variability in gene content implies that strain resolution must be considered in order to fully assess the functional potential of an individual's human gut microbiome.
25896518	9	5	theme	gene	1657:1660	arg1	content					1662:1668	gene content	1657:1668	gene content	1657:1668	The large degree of inter-individual variability in gene content implies that strain resolution must be considered in order to fully assess the functional potential of an individual's human gut microbiome.
25896518	1	6	theme	BACKGROUND	81:90	arg1	differences					105:115	BACKGROUND Gene content differences	81:115	BACKGROUND Gene content differences in human gut microbes	81:137	BACKGROUND Gene content differences in human gut microbes can lead to inter-individual phenotypic variations such as digestive capacity.
25896518	3	7	theme	data	517:520	arg1	use					498:500	the use	494:500	the use of metagenomic data	494:520	Unlike pan-genome studies of cultivable strains, the use of metagenomic data can provide an unbiased view of structural variation of gut bacterial strains by measuring them in their natural habitats, the gut of each individual in this case, representing native boundaries between gut bacterial populations.
25896518	9	8	theme	strain	1683:1688	arg1	resolution					1690:1699	strain resolution	1683:1699	strain resolution	1683:1699	The large degree of inter-individual variability in gene content implies that strain resolution must be considered in order to fully assess the functional potential of an individual's human gut microbiome.
25896518	6	9	theme	lower	1119:1123	arg1	limit					1125:1129	a lower limit	1117:1129	a lower limit	1117:1129	This number is based on gene deletions only and represents a lower limit, yet the variation is already in a similar range as observed between completely sequenced strains of cultivable species.
25896518	5	10	theme	species	938:944	arg1	comparison					898:907	A comparison	896:907	RESULTS A comparison of 11 abundant gut bacterial species	888:944	RESULTS A comparison of 11 abundant gut bacterial species showed that the gene content of strains from the same species differed, on average, by 13% between individuals.
25896518	3	11	theme	native	699:704	arg1	boundaries					706:715	native boundaries	699:715	native boundaries between gut bacterial populations	699:749	Unlike pan-genome studies of cultivable strains, the use of metagenomic data can provide an unbiased view of structural variation of gut bacterial strains by measuring them in their natural habitats, the gut of each individual in this case, representing native boundaries between gut bacterial populations.
25896518	3	12	theme	unbiased	537:544	arg1	view					546:549	an unbiased view	534:549	an unbiased view of structural variation of gut bacterial strains	534:598	Unlike pan-genome studies of cultivable strains, the use of metagenomic data can provide an unbiased view of structural variation of gut bacterial strains by measuring them in their natural habitats, the gut of each individual in this case, representing native boundaries between gut bacterial populations.
25896518	5	13	from	species	1000:1006	arg1	strains					978:984	strains	978:984	strains from the same species	978:1006	RESULTS A comparison of 11 abundant gut bacterial species showed that the gene content of strains from the same species differed, on average, by 13% between individuals.
25896518	5	13	from	species	1000:1006	arg1	content					967:973	the gene content	958:973	the gene content of strains from the same species	958:1006	RESULTS A comparison of 11 abundant gut bacterial species showed that the gene content of strains from the same species differed, on average, by 13% between individuals.
25896518	3	14	theme	gut	578:580	arg1	strains					592:598	gut bacterial strains	578:598	gut bacterial strains	578:598	Unlike pan-genome studies of cultivable strains, the use of metagenomic data can provide an unbiased view of structural variation of gut bacterial strains by measuring them in their natural habitats, the gut of each individual in this case, representing native boundaries between gut bacterial populations.
25896518	4	15	from	samples	811:817	arg1	data					795:798	publicly available metagenomic data	764:798	publicly available metagenomic data from fecal samples	764:817	We analyzed publicly available metagenomic data from fecal samples to characterize inter-individual variation in gut bacterial species.
25896518	8	16	theme	CONCLUSION	1437:1446	arg1	Metagenomics					1448:1459	CONCLUSION Metagenomics	1437:1459	CONCLUSION Metagenomics	1437:1459	CONCLUSION Metagenomics can yield insights into gene content variation of strains in complex communities, which cannot be predicted by phylogenetic marker genes alone.
25896518	6	17	theme	similar	1166:1172	arg1	range					1174:1178	a similar range	1164:1178	a similar range as observed between completely sequenced strains of cultivable species	1164:1249	This number is based on gene deletions only and represents a lower limit, yet the variation is already in a similar range as observed between completely sequenced strains of cultivable species.
25896518	3	18	theme	bacterial	729:737	arg1	populations					739:749	gut bacterial populations	725:749	gut bacterial populations	725:749	Unlike pan-genome studies of cultivable strains, the use of metagenomic data can provide an unbiased view of structural variation of gut bacterial strains by measuring them in their natural habitats, the gut of each individual in this case, representing native boundaries between gut bacterial populations.
25896518	3	19	theme	variation	565:573	arg1	view					546:549	an unbiased view	534:549	an unbiased view of structural variation of gut bacterial strains	534:598	Unlike pan-genome studies of cultivable strains, the use of metagenomic data can provide an unbiased view of structural variation of gut bacterial strains by measuring them in their natural habitats, the gut of each individual in this case, representing native boundaries between gut bacterial populations.
25896518	1	20	theme	human	120:124	arg1	microbes					130:137	human gut microbes	120:137	human gut microbes	120:137	BACKGROUND Gene content differences in human gut microbes can lead to inter-individual phenotypic variations such as digestive capacity.
25896518	2	21	from	differences	276:286	arg1	composition					309:319	microbial species composition	291:319	microbial species composition	291:319	It is unclear whether gene content variation is caused by differences in microbial species composition or by the presence of different strains of the same species; the extent of gene content variation in the latter is unknown.
25896518	5	22	theme	gut	924:926	arg1	species					938:944	11 abundant gut bacterial species	912:944	11 abundant gut bacterial species	912:944	RESULTS A comparison of 11 abundant gut bacterial species showed that the gene content of strains from the same species differed, on average, by 13% between individuals.
25896518	5	23	theme	abundant	915:922	arg1	species					938:944	11 abundant gut bacterial species	912:944	11 abundant gut bacterial species	912:944	RESULTS A comparison of 11 abundant gut bacterial species showed that the gene content of strains from the same species differed, on average, by 13% between individuals.
25896518	0	24	theme	gut	58:60	arg1	species					72:78	human gut bacterial species	52:78	human gut bacterial species	52:78	Inter-individual differences in the gene content of human gut bacterial species.
25896518	3	25	theme	strains	592:598	arg1	variation					565:573	structural variation	554:573	structural variation of gut bacterial strains	554:598	Unlike pan-genome studies of cultivable strains, the use of metagenomic data can provide an unbiased view of structural variation of gut bacterial strains by measuring them in their natural habitats, the gut of each individual in this case, representing native boundaries between gut bacterial populations.
25896518	8	26	theme	gene	1485:1488	arg1	variation					1498:1506	gene content variation	1485:1506	gene content variation of strains in complex communities, which cannot be predicted by phylogenetic marker genes alone	1485:1602	CONCLUSION Metagenomics can yield insights into gene content variation of strains in complex communities, which cannot be predicted by phylogenetic marker genes alone.
25896518	0	27	theme	species	72:78	arg1	content					41:47	the gene content	32:47	the gene content of human gut bacterial species	32:78	Inter-individual differences in the gene content of human gut bacterial species.
25896518	1	28	theme	inter-individual	151:166	arg1	capacity					208:215	digestive capacity	198:215	digestive capacity	198:215	BACKGROUND Gene content differences in human gut microbes can lead to inter-individual phenotypic variations such as digestive capacity.
25896518	1	28	theme	inter-individual	151:166	arg1	variations					179:188	inter-individual phenotypic variations	151:188	inter-individual phenotypic variations such as digestive capacity	151:215	BACKGROUND Gene content differences in human gut microbes can lead to inter-individual phenotypic variations such as digestive capacity.
25896518	2	29	from	latter	426:431	arg1	extent					386:391	the extent	382:391	the extent of gene content variation in the latter	382:431	It is unclear whether gene content variation is caused by differences in microbial species composition or by the presence of different strains of the same species; the extent of gene content variation in the latter is unknown.
25896518	2	29	from	latter	426:431	arg1	unknown					436:442	unknown	436:442	unknown	436:442	It is unclear whether gene content variation is caused by differences in microbial species composition or by the presence of different strains of the same species; the extent of gene content variation in the latter is unknown.
25896518	4	30	from	variation	852:860	arg1	species					879:885	gut bacterial species	865:885	gut bacterial species	865:885	We analyzed publicly available metagenomic data from fecal samples to characterize inter-individual variation in gut bacterial species.
25896518	5	31	theme	gene	962:965	arg1	content					967:973	the gene content	958:973	the gene content of strains from the same species	958:1006	RESULTS A comparison of 11 abundant gut bacterial species showed that the gene content of strains from the same species differed, on average, by 13% between individuals.
25896518	8	32	theme	strains	1511:1517	arg1	variation					1498:1506	gene content variation	1485:1506	gene content variation of strains in complex communities, which cannot be predicted by phylogenetic marker genes alone	1485:1602	CONCLUSION Metagenomics can yield insights into gene content variation of strains in complex communities, which cannot be predicted by phylogenetic marker genes alone.
25896518	2	33	theme	content	401:407	arg1	variation					409:417	gene content variation	396:417	gene content variation in the latter	396:431	It is unclear whether gene content variation is caused by differences in microbial species composition or by the presence of different strains of the same species; the extent of gene content variation in the latter is unknown.
25896518	9	34	theme	gut	1795:1797	arg1	microbiome					1799:1808	an individual's human gut microbiome	1773:1808	an individual's human gut microbiome	1773:1808	The large degree of inter-individual variability in gene content implies that strain resolution must be considered in order to fully assess the functional potential of an individual's human gut microbiome.
25896518	8	35	theme	complex	1522:1528	arg1	communities					1530:1540	complex communities	1522:1540	complex communities	1522:1540	CONCLUSION Metagenomics can yield insights into gene content variation of strains in complex communities, which cannot be predicted by phylogenetic marker genes alone.
25896518	0	36	theme	Inter-individual	0:15	arg1	differences					17:27	Inter-individual differences	0:27	Inter-individual differences in the gene content of human gut bacterial species	0:78	Inter-individual differences in the gene content of human gut bacterial species.
25896518	2	37	theme	content	245:251	arg1	variation					253:261	gene content variation	240:261	gene content variation	240:261	It is unclear whether gene content variation is caused by differences in microbial species composition or by the presence of different strains of the same species; the extent of gene content variation in the latter is unknown.
25896518	1	38	theme	Gene	92:95	arg1	differences					105:115	BACKGROUND Gene content differences	81:115	BACKGROUND Gene content differences in human gut microbes	81:137	BACKGROUND Gene content differences in human gut microbes can lead to inter-individual phenotypic variations such as digestive capacity.
25896518	2	39	theme	variation	409:417	arg1	extent					386:391	the extent	382:391	the extent of gene content variation in the latter	382:431	It is unclear whether gene content variation is caused by differences in microbial species composition or by the presence of different strains of the same species; the extent of gene content variation in the latter is unknown.
25896518	2	39	theme	variation	409:417	arg1	unknown					436:442	unknown	436:442	unknown	436:442	It is unclear whether gene content variation is caused by differences in microbial species composition or by the presence of different strains of the same species; the extent of gene content variation in the latter is unknown.
25896518	3	40	theme	natural	627:633	arg1	habitats					635:642	their natural habitats	621:642	their natural habitats	621:642	Unlike pan-genome studies of cultivable strains, the use of metagenomic data can provide an unbiased view of structural variation of gut bacterial strains by measuring them in their natural habitats, the gut of each individual in this case, representing native boundaries between gut bacterial populations.
25896518	3	40	theme	natural	627:633	arg1	gut					649:651	the gut	645:651	the gut of each individual in this case	645:683	Unlike pan-genome studies of cultivable strains, the use of metagenomic data can provide an unbiased view of structural variation of gut bacterial strains by measuring them in their natural habitats, the gut of each individual in this case, representing native boundaries between gut bacterial populations.
25896518	2	41	theme	gene	396:399	arg1	variation					409:417	gene content variation	396:417	gene content variation in the latter	396:431	It is unclear whether gene content variation is caused by differences in microbial species composition or by the presence of different strains of the same species; the extent of gene content variation in the latter is unknown.
25896518	0	42	theme	gene	36:39	arg1	content					41:47	the gene content	32:47	the gene content of human gut bacterial species	32:78	Inter-individual differences in the gene content of human gut bacterial species.
25896518	8	43	from	variation	1498:1506	arg1	communities					1530:1540	complex communities	1522:1540	complex communities	1522:1540	CONCLUSION Metagenomics can yield insights into gene content variation of strains in complex communities, which cannot be predicted by phylogenetic marker genes alone.
25896518	3	44	theme	cultivable	474:483	arg1	strains					485:491	cultivable strains	474:491	cultivable strains	474:491	Unlike pan-genome studies of cultivable strains, the use of metagenomic data can provide an unbiased view of structural variation of gut bacterial strains by measuring them in their natural habitats, the gut of each individual in this case, representing native boundaries between gut bacterial populations.
25896518	8	45	from	strains	1511:1517	arg1	communities					1530:1540	complex communities	1522:1540	complex communities	1522:1540	CONCLUSION Metagenomics can yield insights into gene content variation of strains in complex communities, which cannot be predicted by phylogenetic marker genes alone.
25896518	2	46	from	extent	386:391	arg1	latter					426:431	latter	426:431	latter	426:431	It is unclear whether gene content variation is caused by differences in microbial species composition or by the presence of different strains of the same species; the extent of gene content variation in the latter is unknown.
25896518	8	47	theme	phylogenetic	1572:1583	arg1	genes					1592:1596	phylogenetic marker genes	1572:1596	phylogenetic marker genes alone	1572:1602	CONCLUSION Metagenomics can yield insights into gene content variation of strains in complex communities, which cannot be predicted by phylogenetic marker genes alone.
25896518	1	48	from	differences	105:115	arg1	microbes					130:137	human gut microbes	120:137	human gut microbes	120:137	BACKGROUND Gene content differences in human gut microbes can lead to inter-individual phenotypic variations such as digestive capacity.
25896518	4	49	theme	metagenomic	783:793	arg1	data					795:798	publicly available metagenomic data	764:798	publicly available metagenomic data from fecal samples	764:817	We analyzed publicly available metagenomic data from fecal samples to characterize inter-individual variation in gut bacterial species.
25896518	2	50	theme	same	368:371	arg1	species					373:379	the same species	364:379	the same species	364:379	It is unclear whether gene content variation is caused by differences in microbial species composition or by the presence of different strains of the same species; the extent of gene content variation in the latter is unknown.
25896518	5	51	theme	same	995:998	arg1	species					1000:1006	the same species	991:1006	the same species	991:1006	RESULTS A comparison of 11 abundant gut bacterial species showed that the gene content of strains from the same species differed, on average, by 13% between individuals.
25896518	2	52	from	variation	409:417	arg1	latter					426:431	latter	426:431	latter	426:431	It is unclear whether gene content variation is caused by differences in microbial species composition or by the presence of different strains of the same species; the extent of gene content variation in the latter is unknown.
25896518	6	53	theme	species	1243:1249	arg1	strains					1221:1227	completely sequenced strains	1200:1227	completely sequenced strains of cultivable species	1200:1249	This number is based on gene deletions only and represents a lower limit, yet the variation is already in a similar range as observed between completely sequenced strains of cultivable species.
25896518	2	54	theme	microbial	291:299	arg1	composition					309:319	microbial species composition	291:319	microbial species composition	291:319	It is unclear whether gene content variation is caused by differences in microbial species composition or by the presence of different strains of the same species; the extent of gene content variation in the latter is unknown.
25896518	4	55	theme	bacterial	869:877	arg1	species					879:885	gut bacterial species	865:885	gut bacterial species	865:885	We analyzed publicly available metagenomic data from fecal samples to characterize inter-individual variation in gut bacterial species.
25896518	7	56	theme	synthesis	1421:1429	arg1	loci					1431:1434	synthesis loci	1421:1434	synthesis loci	1421:1434	We show that accessory genes that differ considerably between individuals can encode important functions, such as polysaccharide utilization and capsular polysaccharide synthesis loci.
25896518	2	57	theme	different	343:351	arg1	strains					353:359	different strains	343:359	different strains of the same species	343:379	It is unclear whether gene content variation is caused by differences in microbial species composition or by the presence of different strains of the same species; the extent of gene content variation in the latter is unknown.
25896518	6	58	theme	sequenced	1211:1219	arg1	strains					1221:1227	completely sequenced strains	1200:1227	completely sequenced strains of cultivable species	1200:1249	This number is based on gene deletions only and represents a lower limit, yet the variation is already in a similar range as observed between completely sequenced strains of cultivable species.
25896518	3	59	theme	metagenomic	505:515	arg1	data					517:520	metagenomic data	505:520	metagenomic data	505:520	Unlike pan-genome studies of cultivable strains, the use of metagenomic data can provide an unbiased view of structural variation of gut bacterial strains by measuring them in their natural habitats, the gut of each individual in this case, representing native boundaries between gut bacterial populations.
25896518	1	60	theme	phenotypic	168:177	arg1	capacity					208:215	digestive capacity	198:215	digestive capacity	198:215	BACKGROUND Gene content differences in human gut microbes can lead to inter-individual phenotypic variations such as digestive capacity.
25896518	1	60	theme	phenotypic	168:177	arg1	variations					179:188	inter-individual phenotypic variations	151:188	inter-individual phenotypic variations such as digestive capacity	151:215	BACKGROUND Gene content differences in human gut microbes can lead to inter-individual phenotypic variations such as digestive capacity.
25896518	7	61	theme	capsular	1397:1404	arg1	polysaccharide					1406:1419	capsular polysaccharide	1397:1419	capsular polysaccharide	1397:1419	We show that accessory genes that differ considerably between individuals can encode important functions, such as polysaccharide utilization and capsular polysaccharide synthesis loci.
25896518	4	62	theme	inter-individual	835:850	arg1	variation					852:860	inter-individual variation	835:860	inter-individual variation in gut bacterial species	835:885	We analyzed publicly available metagenomic data from fecal samples to characterize inter-individual variation in gut bacterial species.
25896518	3	63	theme	gut	725:727	arg1	populations					739:749	gut bacterial populations	725:749	gut bacterial populations	725:749	Unlike pan-genome studies of cultivable strains, the use of metagenomic data can provide an unbiased view of structural variation of gut bacterial strains by measuring them in their natural habitats, the gut of each individual in this case, representing native boundaries between gut bacterial populations.
25896518	1	64	theme	digestive	198:206	arg1	capacity					208:215	digestive capacity	198:215	digestive capacity	198:215	BACKGROUND Gene content differences in human gut microbes can lead to inter-individual phenotypic variations such as digestive capacity.
25896518	2	65	theme	gene	240:243	arg1	variation					253:261	gene content variation	240:261	gene content variation	240:261	It is unclear whether gene content variation is caused by differences in microbial species composition or by the presence of different strains of the same species; the extent of gene content variation in the latter is unknown.
25896518	3	66	theme	structural	554:563	arg1	variation					565:573	structural variation	554:573	structural variation of gut bacterial strains	554:598	Unlike pan-genome studies of cultivable strains, the use of metagenomic data can provide an unbiased view of structural variation of gut bacterial strains by measuring them in their natural habitats, the gut of each individual in this case, representing native boundaries between gut bacterial populations.
25896518	7	67	theme	important	1337:1345	arg1	polysaccharide					1366:1379	polysaccharide utilization and capsular polysaccharide synthesis loci	1366:1434	polysaccharide	1366:1379	We show that accessory genes that differ considerably between individuals can encode important functions, such as polysaccharide utilization and capsular polysaccharide synthesis loci.
25896518	7	67	theme	important	1337:1345	arg1	genes					1275:1279	accessory genes	1265:1279	accessory genes that differ considerably between individuals	1265:1324	We show that accessory genes that differ considerably between individuals can encode important functions, such as polysaccharide utilization and capsular polysaccharide synthesis loci.
25896518	7	67	theme	important	1337:1345	arg1	polysaccharide					1406:1419	capsular polysaccharide	1397:1419	capsular polysaccharide	1397:1419	We show that accessory genes that differ considerably between individuals can encode important functions, such as polysaccharide utilization and capsular polysaccharide synthesis loci.
25896518	7	67	theme	important	1337:1345	arg1	functions					1347:1355	important functions	1337:1355	important functions	1337:1355	We show that accessory genes that differ considerably between individuals can encode important functions, such as polysaccharide utilization and capsular polysaccharide synthesis loci.
25896518	9	68	theme	large	1609:1613	arg1	degree					1615:1620	The large degree	1605:1620	The large degree of inter-individual variability in gene content	1605:1668	The large degree of inter-individual variability in gene content implies that strain resolution must be considered in order to fully assess the functional potential of an individual's human gut microbiome.
25896518	1	69	theme	gut	126:128	arg1	microbes					130:137	human gut microbes	120:137	human gut microbes	120:137	BACKGROUND Gene content differences in human gut microbes can lead to inter-individual phenotypic variations such as digestive capacity.
25896518	0	70	theme	bacterial	62:70	arg1	species					72:78	human gut bacterial species	52:78	human gut bacterial species	52:78	Inter-individual differences in the gene content of human gut bacterial species.
25896518	3	71	theme	bacterial	582:590	arg1	strains					592:598	gut bacterial strains	578:598	gut bacterial strains	578:598	Unlike pan-genome studies of cultivable strains, the use of metagenomic data can provide an unbiased view of structural variation of gut bacterial strains by measuring them in their natural habitats, the gut of each individual in this case, representing native boundaries between gut bacterial populations.
25896518	9	72	theme	functional	1749:1758	arg1	potential					1760:1768	the functional potential	1745:1768	the functional potential of an individual's human gut microbiome	1745:1808	The large degree of inter-individual variability in gene content implies that strain resolution must be considered in order to fully assess the functional potential of an individual's human gut microbiome.
25896518	8	73	theme	content	1490:1496	arg1	variation					1498:1506	gene content variation	1485:1506	gene content variation of strains in complex communities, which cannot be predicted by phylogenetic marker genes alone	1485:1602	CONCLUSION Metagenomics can yield insights into gene content variation of strains in complex communities, which cannot be predicted by phylogenetic marker genes alone.
25896518	7	74	dep	polysaccharide	1366:1379	arg1	loci					1431:1434	synthesis loci	1421:1434	synthesis loci	1421:1434	We show that accessory genes that differ considerably between individuals can encode important functions, such as polysaccharide utilization and capsular polysaccharide synthesis loci.
25896518	7	74	dep	polysaccharide	1366:1379	arg1	utilization					1381:1391	utilization	1381:1391	utilization	1381:1391	We show that accessory genes that differ considerably between individuals can encode important functions, such as polysaccharide utilization and capsular polysaccharide synthesis loci.
25896518	0	75	from	differences	17:27	arg1	content					41:47	the gene content	32:47	the gene content of human gut bacterial species	32:78	Inter-individual differences in the gene content of human gut bacterial species.
25896518	2	76	theme	species	301:307	arg1	composition					309:319	microbial species composition	291:319	microbial species composition	291:319	It is unclear whether gene content variation is caused by differences in microbial species composition or by the presence of different strains of the same species; the extent of gene content variation in the latter is unknown.
25896518	9	77	theme	human	1789:1793	arg1	microbiome					1799:1808	an individual's human gut microbiome	1773:1808	an individual's human gut microbiome	1773:1808	The large degree of inter-individual variability in gene content implies that strain resolution must be considered in order to fully assess the functional potential of an individual's human gut microbiome.
25896518	5	78	theme	bacterial	928:936	arg1	species					938:944	11 abundant gut bacterial species	912:944	11 abundant gut bacterial species	912:944	RESULTS A comparison of 11 abundant gut bacterial species showed that the gene content of strains from the same species differed, on average, by 13% between individuals.
25896518	9	79	from	degree	1615:1620	arg1	content					1662:1668	gene content	1657:1668	gene content	1657:1668	The large degree of inter-individual variability in gene content implies that strain resolution must be considered in order to fully assess the functional potential of an individual's human gut microbiome.
25896518	7	80	theme	accessory	1265:1273	arg1	polysaccharide					1366:1379	polysaccharide utilization and capsular polysaccharide synthesis loci	1366:1434	polysaccharide	1366:1379	We show that accessory genes that differ considerably between individuals can encode important functions, such as polysaccharide utilization and capsular polysaccharide synthesis loci.
25896518	7	80	theme	accessory	1265:1273	arg1	genes					1275:1279	accessory genes	1265:1279	accessory genes that differ considerably between individuals	1265:1324	We show that accessory genes that differ considerably between individuals can encode important functions, such as polysaccharide utilization and capsular polysaccharide synthesis loci.
25896518	7	80	theme	accessory	1265:1273	arg1	polysaccharide					1406:1419	capsular polysaccharide	1397:1419	capsular polysaccharide	1397:1419	We show that accessory genes that differ considerably between individuals can encode important functions, such as polysaccharide utilization and capsular polysaccharide synthesis loci.
25896518	7	80	theme	accessory	1265:1273	arg1	functions					1347:1355	important functions	1337:1355	important functions	1337:1355	We show that accessory genes that differ considerably between individuals can encode important functions, such as polysaccharide utilization and capsular polysaccharide synthesis loci.
25896518	9	81	theme	microbiome	1799:1808	arg1	potential					1760:1768	the functional potential	1745:1768	the functional potential of an individual's human gut microbiome	1745:1808	The large degree of inter-individual variability in gene content implies that strain resolution must be considered in order to fully assess the functional potential of an individual's human gut microbiome.
25896518	2	82	theme	species	373:379	arg1	strains					353:359	different strains	343:359	different strains of the same species	343:379	It is unclear whether gene content variation is caused by differences in microbial species composition or by the presence of different strains of the same species; the extent of gene content variation in the latter is unknown.
25896518	1	83	theme	content	97:103	arg1	differences					105:115	BACKGROUND Gene content differences	81:115	BACKGROUND Gene content differences in human gut microbes	81:137	BACKGROUND Gene content differences in human gut microbes can lead to inter-individual phenotypic variations such as digestive capacity.
25896518	5	84	dep	RESULTS	888:894	arg1	comparison					898:907	A comparison	896:907	RESULTS A comparison of 11 abundant gut bacterial species	888:944	RESULTS A comparison of 11 abundant gut bacterial species showed that the gene content of strains from the same species differed, on average, by 13% between individuals.
25896518	3	85	theme	pan-genome	452:461	arg1	studies					463:469	pan-genome studies	452:469	pan-genome studies of cultivable strains	452:491	Unlike pan-genome studies of cultivable strains, the use of metagenomic data can provide an unbiased view of structural variation of gut bacterial strains by measuring them in their natural habitats, the gut of each individual in this case, representing native boundaries between gut bacterial populations.
25896518	8	86	from	communities	1530:1540	arg1	variation					1498:1506	gene content variation	1485:1506	gene content variation of strains in complex communities, which cannot be predicted by phylogenetic marker genes alone	1485:1602	CONCLUSION Metagenomics can yield insights into gene content variation of strains in complex communities, which cannot be predicted by phylogenetic marker genes alone.
25896518	0	87	theme	human	52:56	arg1	species					72:78	human gut bacterial species	52:78	human gut bacterial species	52:78	Inter-individual differences in the gene content of human gut bacterial species.
25896518	8	88	theme	marker	1585:1590	arg1	genes					1592:1596	phylogenetic marker genes	1572:1596	phylogenetic marker genes alone	1572:1602	CONCLUSION Metagenomics can yield insights into gene content variation of strains in complex communities, which cannot be predicted by phylogenetic marker genes alone.
25896518	4	89	theme	fecal	805:809	arg1	samples					811:817	fecal samples	805:817	fecal samples	805:817	We analyzed publicly available metagenomic data from fecal samples to characterize inter-individual variation in gut bacterial species.
25896518	3	90	theme	strains	485:491	arg1	studies					463:469	pan-genome studies	452:469	pan-genome studies of cultivable strains	452:491	Unlike pan-genome studies of cultivable strains, the use of metagenomic data can provide an unbiased view of structural variation of gut bacterial strains by measuring them in their natural habitats, the gut of each individual in this case, representing native boundaries between gut bacterial populations.
25896518	9	91	theme	inter-individual	1625:1640	arg1	variability					1642:1652	inter-individual variability	1625:1652	inter-individual variability	1625:1652	The large degree of inter-individual variability in gene content implies that strain resolution must be considered in order to fully assess the functional potential of an individual's human gut microbiome.
25896518	5	92	theme	strains	978:984	arg1	content					967:973	the gene content	958:973	the gene content of strains from the same species	958:1006	RESULTS A comparison of 11 abundant gut bacterial species showed that the gene content of strains from the same species differed, on average, by 13% between individuals.
25896518	6	93	theme	gene	1082:1085	arg1	deletions					1087:1095	gene deletions	1082:1095	gene deletions	1082:1095	This number is based on gene deletions only and represents a lower limit, yet the variation is already in a similar range as observed between completely sequenced strains of cultivable species.
25896518	3	94	theme	individual	661:670	arg1	habitats					635:642	their natural habitats	621:642	their natural habitats	621:642	Unlike pan-genome studies of cultivable strains, the use of metagenomic data can provide an unbiased view of structural variation of gut bacterial strains by measuring them in their natural habitats, the gut of each individual in this case, representing native boundaries between gut bacterial populations.
25896518	3	94	theme	individual	661:670	arg1	gut					649:651	the gut	645:651	the gut of each individual in this case	645:683	Unlike pan-genome studies of cultivable strains, the use of metagenomic data can provide an unbiased view of structural variation of gut bacterial strains by measuring them in their natural habitats, the gut of each individual in this case, representing native boundaries between gut bacterial populations.
25896518	4	95	theme	available	773:781	arg1	data					795:798	publicly available metagenomic data	764:798	publicly available metagenomic data from fecal samples	764:817	We analyzed publicly available metagenomic data from fecal samples to characterize inter-individual variation in gut bacterial species.
25896518	5	96	from	content	967:973	arg1	species					1000:1006	the same species	991:1006	the same species	991:1006	RESULTS A comparison of 11 abundant gut bacterial species showed that the gene content of strains from the same species differed, on average, by 13% between individuals.
28569438	10	0	theme	mineralization	1594:1607	arg1	media					1609:1613	the respective mineralization media	1579:1613	the respective mineralization media	1579:1613	The calcium:magnesium elemental ratio in the mineral formed was higher than in the respective mineralization media.
28569438	11	1	theme	osteoblast-like	1710:1724	arg1	cells					1726:1730	osteoblast-like cells	1710:1730	osteoblast-like cells	1710:1730	Mineralization of hydrogels with calcite or magnesian calcite promoted adhesion and growth of osteoblast-like cells.
28569438	2	2	theme	regeneration	382:393	arg1	material					395:402	a bone regeneration material	375:402	a bone regeneration material	375:402	Calcium carbonate (CaCO3 ) has been successfully applied as a bone regeneration material, but hydrogel-CaCO3 composites have received less attention.
28569438	2	2	theme	regeneration	382:393	arg1	carbonate					323:331	Calcium carbonate	315:331	Calcium carbonate (CaCO3 )	315:340	Calcium carbonate (CaCO3 ) has been successfully applied as a bone regeneration material, but hydrogel-CaCO3 composites have received less attention.
28569438	4	3	from	study	733:737	arg1	mineralized					771:781	mineralized	771:781	mineralized	771:781	In the present study, gellan gum (GG) hydrogels were mineralized enzymatically with CaCO3 , Mg-enriched CaCO3 and magnesium carbonate to generate composite biomaterials for bone regeneration.
28569438	1	4	theme	bone	296:299	arg1	regeneration					301:312	bone regeneration	296:312	bone regeneration	296:312	Mineralization of hydrogel biomaterials is considered desirable to improve their suitability as materials for bone regeneration.
28569438	13	5	theme	calcite	1888:1894	arg1	form					1880:1883	the form	1876:1883	the form of calcite	1876:1894	In conclusion, enzymatic mineralization of GG hydrogels with CaCO3 in the form of calcite successfully reinforced hydrogels and promoted osteoblast-like cell adhesion and growth, but magnesium enrichment had no definitive positive effect.
28569438	8	6	theme	Mg5	1274:1276	arg1	OH					1287:1288	OH	1287:1288	OH	1287:1288	Hydromagnesite (Mg5 (CO3 )4 (OH)2 .4H2 O) formed at high magnesium concentration in the absence of calcium.
28569438	8	6	theme	Mg5	1274:1276	arg1	CO3					1279:1281	Mg5 (CO3 )4	1274:1284	Mg5 (CO3 )4 (OH)2 .4H2 O	1274:1297	Hydromagnesite (Mg5 (CO3 )4 (OH)2 .4H2 O) formed at high magnesium concentration in the absence of calcium.
28569438	4	7	theme	bone	891:894	arg1	regeneration					896:907	bone regeneration	891:907	bone regeneration	891:907	In the present study, gellan gum (GG) hydrogels were mineralized enzymatically with CaCO3 , Mg-enriched CaCO3 and magnesium carbonate to generate composite biomaterials for bone regeneration.
28569438	2	8	theme	Calcium	315:321	arg1	material					395:402	a bone regeneration material	375:402	a bone regeneration material	375:402	Calcium carbonate (CaCO3 ) has been successfully applied as a bone regeneration material, but hydrogel-CaCO3 composites have received less attention.
28569438	2	8	theme	Calcium	315:321	arg1	CaCO3					334:338	CaCO3	334:338	CaCO3	334:338	Calcium carbonate (CaCO3 ) has been successfully applied as a bone regeneration material, but hydrogel-CaCO3 composites have received less attention.
28569438	2	8	theme	Calcium	315:321	arg1	carbonate					323:331	Calcium carbonate	315:331	Calcium carbonate (CaCO3 )	315:340	Calcium carbonate (CaCO3 ) has been successfully applied as a bone regeneration material, but hydrogel-CaCO3 composites have received less attention.
28569438	1	9	theme	hydrogel	204:211	arg1	biomaterials					213:224	hydrogel biomaterials	204:224	hydrogel biomaterials	204:224	Mineralization of hydrogel biomaterials is considered desirable to improve their suitability as materials for bone regeneration.
28569438	13	10	theme	osteoblast-like	1943:1957	arg1	adhesion					1964:1971	osteoblast-like cell adhesion	1943:1971	osteoblast-like cell adhesion	1943:1971	In conclusion, enzymatic mineralization of GG hydrogels with CaCO3 in the form of calcite successfully reinforced hydrogels and promoted osteoblast-like cell adhesion and growth, but magnesium enrichment had no definitive positive effect.
28569438	8	11	theme	high	1310:1313	arg1	concentration					1325:1337	high magnesium concentration	1310:1337	high magnesium concentration	1310:1337	Hydromagnesite (Mg5 (CO3 )4 (OH)2 .4H2 O) formed at high magnesium concentration in the absence of calcium.
28569438	0	12	theme	magnesium	130:138	arg1	carbonate					140:148	magnesium carbonate	130:148	magnesium carbonate for bone regeneration applications	130:183	Enzymatic, urease-mediated mineralization of gellan gum hydrogel with calcium carbonate, magnesium-enriched calcium carbonate and magnesium carbonate for bone regeneration applications.
28569438	3	13	theme	bone	605:608	arg1	regeneration					610:621	bone regeneration	605:621	bone regeneration	605:621	Magnesium (Mg) has been used as a component of calcium phosphate biomaterials to stimulate bone-forming cell adhesion and proliferation and bone regeneration in vivo, but its effect as a component of carbonate-based biomaterials remains uninvestigated.
28569438	4	14	theme	gum	747:749	arg1	hydrogels					756:764	gellan gum (GG) hydrogels	740:764	gellan gum (GG) hydrogels	740:764	In the present study, gellan gum (GG) hydrogels were mineralized enzymatically with CaCO3 , Mg-enriched CaCO3 and magnesium carbonate to generate composite biomaterials for bone regeneration.
28569438	13	15	theme	GG	1849:1850	arg1	hydrogels					1852:1860	GG hydrogels	1849:1860	GG hydrogels with CaCO3	1849:1871	In conclusion, enzymatic mineralization of GG hydrogels with CaCO3 in the form of calcite successfully reinforced hydrogels and promoted osteoblast-like cell adhesion and growth, but magnesium enrichment had no definitive positive effect.
28569438	7	16	theme	low	1148:1150	arg1	concentrations					1162:1175	low magnesium concentrations	1148:1175	low magnesium concentrations	1148:1175	At low magnesium concentrations calcite was formed, while at higher concentrations magnesian calcite was formed.
28569438	0	17	theme	regeneration	159:170	arg1	applications					172:183	bone regeneration applications	154:183	bone regeneration applications	154:183	Enzymatic, urease-mediated mineralization of gellan gum hydrogel with calcium carbonate, magnesium-enriched calcium carbonate and magnesium carbonate for bone regeneration applications.
28569438	9	18	theme	mineralization	1477:1490	arg1	medium					1492:1497	the mineralization medium	1473:1497	the mineralization medium	1473:1497	The amount of mineral formed and compressive strength decreased with increasing magnesium concentration in the mineralization medium.
28569438	5	19	theme	mineralization	984:997	arg1	media					999:1003	mineralization media	984:1003	mineralization media containing urea	984:1019	Hydrogels loaded with the enzyme urease were mineralized by incubation in mineralization media containing urea and different ratios of calcium and magnesium ions.
28569438	6	20	theme	magnesium	1088:1096	arg1	concentration					1098:1110	the magnesium concentration	1084:1110	the magnesium concentration	1084:1110	Increasing the magnesium concentration decreased mineral crystallinity.
28569438	10	21	theme	magnesium	1512:1520	arg1	ratio					1532:1536	The calcium:magnesium elemental ratio	1500:1536	The calcium:magnesium elemental ratio in the mineral formed	1500:1558	The calcium:magnesium elemental ratio in the mineral formed was higher than in the respective mineralization media.
28569438	10	21	theme	magnesium	1512:1520	arg1	higher					1564:1569	higher	1564:1569	higher	1564:1569	The calcium:magnesium elemental ratio in the mineral formed was higher than in the respective mineralization media.
28569438	4	22	theme	gellan	740:745	arg1	GG					752:753	GG	752:753	GG	752:753	In the present study, gellan gum (GG) hydrogels were mineralized enzymatically with CaCO3 , Mg-enriched CaCO3 and magnesium carbonate to generate composite biomaterials for bone regeneration.
28569438	4	22	theme	gellan	740:745	arg1	gum					747:749	gellan gum	740:749	gellan gum (GG) hydrogels	740:764	In the present study, gellan gum (GG) hydrogels were mineralized enzymatically with CaCO3 , Mg-enriched CaCO3 and magnesium carbonate to generate composite biomaterials for bone regeneration.
28569438	13	23	theme	enzymatic	1821:1829	arg1	mineralization					1831:1844	enzymatic mineralization	1821:1844	enzymatic mineralization of GG hydrogels with CaCO3 in the form of calcite	1821:1894	In conclusion, enzymatic mineralization of GG hydrogels with CaCO3 in the form of calcite successfully reinforced hydrogels and promoted osteoblast-like cell adhesion and growth, but magnesium enrichment had no definitive positive effect.
28569438	10	24	theme	calcium	1504:1510	arg1	ratio					1532:1536	The calcium:magnesium elemental ratio	1500:1536	The calcium:magnesium elemental ratio in the mineral formed	1500:1558	The calcium:magnesium elemental ratio in the mineral formed was higher than in the respective mineralization media.
28569438	10	24	theme	calcium	1504:1510	arg1	higher					1564:1569	higher	1564:1569	higher	1564:1569	The calcium:magnesium elemental ratio in the mineral formed was higher than in the respective mineralization media.
28569438	13	25	with	hydrogels	1852:1860	arg1	CaCO3					1867:1871	CaCO3	1867:1871	CaCO3	1867:1871	In conclusion, enzymatic mineralization of GG hydrogels with CaCO3 in the form of calcite successfully reinforced hydrogels and promoted osteoblast-like cell adhesion and growth, but magnesium enrichment had no definitive positive effect.
28569438	13	26	theme	positive	2028:2035	arg1	effect					2037:2042	no definitive positive effect	2014:2042	no definitive positive effect	2014:2042	In conclusion, enzymatic mineralization of GG hydrogels with CaCO3 in the form of calcite successfully reinforced hydrogels and promoted osteoblast-like cell adhesion and growth, but magnesium enrichment had no definitive positive effect.
28569438	0	27	theme	magnesium-enriched	89:106	arg1	carbonate					78:86	calcium carbonate	70:86	calcium carbonate	70:86	Enzymatic, urease-mediated mineralization of gellan gum hydrogel with calcium carbonate, magnesium-enriched calcium carbonate and magnesium carbonate for bone regeneration applications.
28569438	0	27	theme	magnesium-enriched	89:106	arg1	carbonate					116:124	magnesium-enriched calcium carbonate	89:124	magnesium-enriched calcium carbonate	89:124	Enzymatic, urease-mediated mineralization of gellan gum hydrogel with calcium carbonate, magnesium-enriched calcium carbonate and magnesium carbonate for bone regeneration applications.
28569438	4	28	theme	Mg-enriched	810:820	arg1	CaCO3					822:826	Mg-enriched CaCO3	810:826	Mg-enriched CaCO3	810:826	In the present study, gellan gum (GG) hydrogels were mineralized enzymatically with CaCO3 , Mg-enriched CaCO3 and magnesium carbonate to generate composite biomaterials for bone regeneration.
28569438	5	29	theme	calcium	1045:1051	arg1	ions					1067:1070	calcium and magnesium ions	1045:1070	calcium and magnesium ions	1045:1070	Hydrogels loaded with the enzyme urease were mineralized by incubation in mineralization media containing urea and different ratios of calcium and magnesium ions.
28569438	13	30	contain	had	2010:2012	arg2	effect					2037:2042	no definitive positive effect	2014:2042	no definitive positive effect	2014:2042	In conclusion, enzymatic mineralization of GG hydrogels with CaCO3 in the form of calcite successfully reinforced hydrogels and promoted osteoblast-like cell adhesion and growth, but magnesium enrichment had no definitive positive effect.
28569438	13	30	contain	had	2010:2012	arg1	enrichment					1999:2008	magnesium enrichment	1989:2008	magnesium enrichment	1989:2008	In conclusion, enzymatic mineralization of GG hydrogels with CaCO3 in the form of calcite successfully reinforced hydrogels and promoted osteoblast-like cell adhesion and growth, but magnesium enrichment had no definitive positive effect.
28569438	3	31	used	used	489:492	arg2	Magnesium					465:473	Magnesium	465:473	Magnesium (Mg)	465:478	Magnesium (Mg) has been used as a component of calcium phosphate biomaterials to stimulate bone-forming cell adhesion and proliferation and bone regeneration in vivo, but its effect as a component of carbonate-based biomaterials remains uninvestigated.
28569438	3	31	used	used	489:492	arg2	component					499:507	a component	497:507	a component of calcium phosphate biomaterials	497:541	Magnesium (Mg) has been used as a component of calcium phosphate biomaterials to stimulate bone-forming cell adhesion and proliferation and bone regeneration in vivo, but its effect as a component of carbonate-based biomaterials remains uninvestigated.
28569438	3	31	used	used	489:492	arg2	Mg					476:477	Mg	476:477	Mg	476:477	Magnesium (Mg) has been used as a component of calcium phosphate biomaterials to stimulate bone-forming cell adhesion and proliferation and bone regeneration in vivo, but its effect as a component of carbonate-based biomaterials remains uninvestigated.
28569438	0	32	theme	Enzymatic	0:8	arg1	mineralization					27:40	Enzymatic, urease-mediated mineralization	0:40	Enzymatic, urease-mediated mineralization of gellan gum hydrogel with calcium carbonate, magnesium-enriched calcium carbonate and magnesium carbonate for bone regeneration applications.	0:184	Enzymatic, urease-mediated mineralization of gellan gum hydrogel with calcium carbonate, magnesium-enriched calcium carbonate and magnesium carbonate for bone regeneration applications.
28569438	11	33	with	hydrogels	1634:1642	arg1	calcite					1670:1676	magnesian calcite	1660:1676	magnesian calcite	1660:1676	Mineralization of hydrogels with calcite or magnesian calcite promoted adhesion and growth of osteoblast-like cells.
28569438	11	33	with	hydrogels	1634:1642	arg1	calcite					1649:1655	calcite	1649:1655	calcite	1649:1655	Mineralization of hydrogels with calcite or magnesian calcite promoted adhesion and growth of osteoblast-like cells.
28569438	9	34	theme	formed	1388:1393	arg1	strength					1411:1418	mineral formed and compressive strength	1380:1418	mineral formed and compressive strength	1380:1418	The amount of mineral formed and compressive strength decreased with increasing magnesium concentration in the mineralization medium.
28569438	9	35	theme	compressive	1399:1409	arg1	strength					1411:1418	mineral formed and compressive strength	1380:1418	mineral formed and compressive strength	1380:1418	The amount of mineral formed and compressive strength decreased with increasing magnesium concentration in the mineralization medium.
28569438	10	36	theme	respective	1583:1592	arg1	media					1609:1613	the respective mineralization media	1579:1613	the respective mineralization media	1579:1613	The calcium:magnesium elemental ratio in the mineral formed was higher than in the respective mineralization media.
28569438	3	37	theme	carbonate-based	665:679	arg1	biomaterials					681:692	carbonate-based biomaterials	665:692	carbonate-based biomaterials	665:692	Magnesium (Mg) has been used as a component of calcium phosphate biomaterials to stimulate bone-forming cell adhesion and proliferation and bone regeneration in vivo, but its effect as a component of carbonate-based biomaterials remains uninvestigated.
28569438	2	38	theme	bone	377:380	arg1	material					395:402	a bone regeneration material	375:402	a bone regeneration material	375:402	Calcium carbonate (CaCO3 ) has been successfully applied as a bone regeneration material, but hydrogel-CaCO3 composites have received less attention.
28569438	2	38	theme	bone	377:380	arg1	carbonate					323:331	Calcium carbonate	315:331	Calcium carbonate (CaCO3 )	315:340	Calcium carbonate (CaCO3 ) has been successfully applied as a bone regeneration material, but hydrogel-CaCO3 composites have received less attention.
28569438	0	39	theme	gum	52:54	arg1	hydrogel					56:63	gellan gum hydrogel	45:63	gellan gum hydrogel	45:63	Enzymatic, urease-mediated mineralization of gellan gum hydrogel with calcium carbonate, magnesium-enriched calcium carbonate and magnesium carbonate for bone regeneration applications.
28569438	12	40	with	mineralized	1743:1753	arg1	hydromagnesite					1760:1773	hydromagnesite	1760:1773	hydromagnesite	1760:1773	Hydrogels mineralized with hydromagnesite displayed higher cytotoxicity.
28569438	8	41	dep	CO3	1279:1281	arg1	O					1297:1297	2 .4H2 O	1290:1297	Mg5 (CO3 )4 (OH)2 .4H2 O	1274:1297	Hydromagnesite (Mg5 (CO3 )4 (OH)2 .4H2 O) formed at high magnesium concentration in the absence of calcium.
28569438	11	42	theme	magnesian	1660:1668	arg1	calcite					1670:1676	magnesian calcite	1660:1676	magnesian calcite	1660:1676	Mineralization of hydrogels with calcite or magnesian calcite promoted adhesion and growth of osteoblast-like cells.
28569438	5	43	theme	magnesium	1057:1065	arg1	ions					1067:1070	calcium and magnesium ions	1045:1070	calcium and magnesium ions	1045:1070	Hydrogels loaded with the enzyme urease were mineralized by incubation in mineralization media containing urea and different ratios of calcium and magnesium ions.
28569438	4	44	theme	present	725:731	arg1	study					733:737	the present study	721:737	the present study	721:737	In the present study, gellan gum (GG) hydrogels were mineralized enzymatically with CaCO3 , Mg-enriched CaCO3 and magnesium carbonate to generate composite biomaterials for bone regeneration.
28569438	7	45	theme	magnesian	1228:1236	arg1	calcite					1238:1244	magnesian calcite	1228:1244	magnesian calcite	1228:1244	At low magnesium concentrations calcite was formed, while at higher concentrations magnesian calcite was formed.
28569438	0	46	dep	Enzymatic	0:8	arg1	urease-mediated					11:25	urease-mediated	11:25	urease-mediated	11:25	Enzymatic, urease-mediated mineralization of gellan gum hydrogel with calcium carbonate, magnesium-enriched calcium carbonate and magnesium carbonate for bone regeneration applications.
28569438	13	47	from	mineralization	1831:1844	arg1	form					1880:1883	the form	1876:1883	the form of calcite	1876:1894	In conclusion, enzymatic mineralization of GG hydrogels with CaCO3 in the form of calcite successfully reinforced hydrogels and promoted osteoblast-like cell adhesion and growth, but magnesium enrichment had no definitive positive effect.
28569438	7	48	theme	higher	1206:1211	arg1	concentrations					1213:1226	higher concentrations	1206:1226	higher concentrations	1206:1226	At low magnesium concentrations calcite was formed, while at higher concentrations magnesian calcite was formed.
28569438	3	49	theme	biomaterials	530:541	arg1	component					499:507	a component	497:507	a component of calcium phosphate biomaterials	497:541	Magnesium (Mg) has been used as a component of calcium phosphate biomaterials to stimulate bone-forming cell adhesion and proliferation and bone regeneration in vivo, but its effect as a component of carbonate-based biomaterials remains uninvestigated.
28569438	3	49	theme	biomaterials	530:541	arg1	Magnesium					465:473	Magnesium	465:473	Magnesium (Mg)	465:478	Magnesium (Mg) has been used as a component of calcium phosphate biomaterials to stimulate bone-forming cell adhesion and proliferation and bone regeneration in vivo, but its effect as a component of carbonate-based biomaterials remains uninvestigated.
28569438	11	50	theme	cells	1726:1730	arg1	growth					1700:1705	growth	1700:1705	growth	1700:1705	Mineralization of hydrogels with calcite or magnesian calcite promoted adhesion and growth of osteoblast-like cells.
28569438	11	50	theme	cells	1726:1730	arg1	adhesion					1687:1694	adhesion	1687:1694	adhesion	1687:1694	Mineralization of hydrogels with calcite or magnesian calcite promoted adhesion and growth of osteoblast-like cells.
28569438	5	51	from	ratios	1035:1040	arg1	media					999:1003	mineralization media	984:1003	mineralization media containing urea	984:1019	Hydrogels loaded with the enzyme urease were mineralized by incubation in mineralization media containing urea and different ratios of calcium and magnesium ions.
28569438	3	52	theme	calcium	512:518	arg1	biomaterials					530:541	calcium phosphate biomaterials	512:541	calcium phosphate biomaterials	512:541	Magnesium (Mg) has been used as a component of calcium phosphate biomaterials to stimulate bone-forming cell adhesion and proliferation and bone regeneration in vivo, but its effect as a component of carbonate-based biomaterials remains uninvestigated.
28569438	3	53	theme	cell	569:572	arg1	adhesion					574:581	bone-forming cell adhesion	556:581	bone-forming cell adhesion	556:581	Magnesium (Mg) has been used as a component of calcium phosphate biomaterials to stimulate bone-forming cell adhesion and proliferation and bone regeneration in vivo, but its effect as a component of carbonate-based biomaterials remains uninvestigated.
28569438	8	54	theme	.4H2	1292:1295	arg1	O					1297:1297	2 .4H2 O	1290:1297	Mg5 (CO3 )4 (OH)2 .4H2 O	1274:1297	Hydromagnesite (Mg5 (CO3 )4 (OH)2 .4H2 O) formed at high magnesium concentration in the absence of calcium.
28569438	1	55	theme	biomaterials	213:224	arg1	Mineralization					186:199	Mineralization	186:199	Mineralization of hydrogel biomaterials	186:224	Mineralization of hydrogel biomaterials is considered desirable to improve their suitability as materials for bone regeneration.
28569438	7	56	theme	magnesium	1152:1160	arg1	concentrations					1162:1175	low magnesium concentrations	1148:1175	low magnesium concentrations	1148:1175	At low magnesium concentrations calcite was formed, while at higher concentrations magnesian calcite was formed.
28569438	11	57	theme	hydrogels	1634:1642	arg1	Mineralization					1616:1629	Mineralization	1616:1629	Mineralization of hydrogels with calcite or magnesian calcite	1616:1676	Mineralization of hydrogels with calcite or magnesian calcite promoted adhesion and growth of osteoblast-like cells.
28569438	5	58	contain	containing	1005:1014	arg1	media					999:1003	mineralization media	984:1003	mineralization media containing urea	984:1019	Hydrogels loaded with the enzyme urease were mineralized by incubation in mineralization media containing urea and different ratios of calcium and magnesium ions.
28569438	5	58	contain	containing	1005:1014	arg2	urea					1016:1019	urea	1016:1019	urea	1016:1019	Hydrogels loaded with the enzyme urease were mineralized by incubation in mineralization media containing urea and different ratios of calcium and magnesium ions.
28569438	0	59	theme	bone	154:157	arg1	applications					172:183	bone regeneration applications	154:183	bone regeneration applications	154:183	Enzymatic, urease-mediated mineralization of gellan gum hydrogel with calcium carbonate, magnesium-enriched calcium carbonate and magnesium carbonate for bone regeneration applications.
28569438	10	60	from	ratio	1532:1536	arg1	mineral					1545:1551	mineral	1545:1551	mineral	1545:1551	The calcium:magnesium elemental ratio in the mineral formed was higher than in the respective mineralization media.
28569438	8	61	theme	magnesium	1315:1323	arg1	concentration					1325:1337	high magnesium concentration	1310:1337	high magnesium concentration	1310:1337	Hydromagnesite (Mg5 (CO3 )4 (OH)2 .4H2 O) formed at high magnesium concentration in the absence of calcium.
28569438	13	62	theme	hydrogels	1852:1860	arg1	mineralization					1831:1844	enzymatic mineralization	1821:1844	enzymatic mineralization of GG hydrogels with CaCO3 in the form of calcite	1821:1894	In conclusion, enzymatic mineralization of GG hydrogels with CaCO3 in the form of calcite successfully reinforced hydrogels and promoted osteoblast-like cell adhesion and growth, but magnesium enrichment had no definitive positive effect.
28569438	4	63	with	mineralized	771:781	arg1	CaCO3					802:806	CaCO3	802:806	CaCO3	802:806	In the present study, gellan gum (GG) hydrogels were mineralized enzymatically with CaCO3 , Mg-enriched CaCO3 and magnesium carbonate to generate composite biomaterials for bone regeneration.
28569438	4	63	with	mineralized	771:781	arg1	CaCO3					822:826	Mg-enriched CaCO3	810:826	Mg-enriched CaCO3	810:826	In the present study, gellan gum (GG) hydrogels were mineralized enzymatically with CaCO3 , Mg-enriched CaCO3 and magnesium carbonate to generate composite biomaterials for bone regeneration.
28569438	4	63	with	mineralized	771:781	arg1	carbonate					842:850	magnesium carbonate	832:850	magnesium carbonate	832:850	In the present study, gellan gum (GG) hydrogels were mineralized enzymatically with CaCO3 , Mg-enriched CaCO3 and magnesium carbonate to generate composite biomaterials for bone regeneration.
28569438	14	64	dep	&	2073:2073	arg1	Sons					2075:2078	Sons	2075:2078	Sons	2075:2078	Copyright © 2017 John Wiley & Sons, Ltd.
28569438	8	65	dep	Hydromagnesite	1258:1271	arg1	OH					1287:1288	OH	1287:1288	OH	1287:1288	Hydromagnesite (Mg5 (CO3 )4 (OH)2 .4H2 O) formed at high magnesium concentration in the absence of calcium.
28569438	8	65	dep	Hydromagnesite	1258:1271	arg1	CO3					1279:1281	Mg5 (CO3 )4	1274:1284	Mg5 (CO3 )4 (OH)2 .4H2 O	1274:1297	Hydromagnesite (Mg5 (CO3 )4 (OH)2 .4H2 O) formed at high magnesium concentration in the absence of calcium.
28569438	0	66	theme	calcium	70:76	arg1	carbonate					78:86	calcium carbonate	70:86	calcium carbonate	70:86	Enzymatic, urease-mediated mineralization of gellan gum hydrogel with calcium carbonate, magnesium-enriched calcium carbonate and magnesium carbonate for bone regeneration applications.
28569438	0	66	theme	calcium	70:76	arg1	carbonate					116:124	magnesium-enriched calcium carbonate	89:124	magnesium-enriched calcium carbonate	89:124	Enzymatic, urease-mediated mineralization of gellan gum hydrogel with calcium carbonate, magnesium-enriched calcium carbonate and magnesium carbonate for bone regeneration applications.
28569438	5	67	theme	enzyme	936:941	arg1	urease					943:948	the enzyme urease	932:948	the enzyme urease	932:948	Hydrogels loaded with the enzyme urease were mineralized by incubation in mineralization media containing urea and different ratios of calcium and magnesium ions.
28569438	9	68	dep	formed	1388:1393	arg1	mineral					1380:1386	mineral	1380:1386	mineral	1380:1386	The amount of mineral formed and compressive strength decreased with increasing magnesium concentration in the mineralization medium.
28569438	8	69	theme	calcium	1357:1363	arg1	absence					1346:1352	the absence	1342:1352	the absence of calcium	1342:1363	Hydromagnesite (Mg5 (CO3 )4 (OH)2 .4H2 O) formed at high magnesium concentration in the absence of calcium.
28569438	12	70	theme	higher	1785:1790	arg1	cytotoxicity					1792:1803	higher cytotoxicity	1785:1803	higher cytotoxicity	1785:1803	Hydrogels mineralized with hydromagnesite displayed higher cytotoxicity.
28569438	13	71	theme	definitive	2017:2026	arg1	effect					2037:2042	no definitive positive effect	2014:2042	no definitive positive effect	2014:2042	In conclusion, enzymatic mineralization of GG hydrogels with CaCO3 in the form of calcite successfully reinforced hydrogels and promoted osteoblast-like cell adhesion and growth, but magnesium enrichment had no definitive positive effect.
28569438	0	72	theme	calcium	108:114	arg1	carbonate					78:86	calcium carbonate	70:86	calcium carbonate	70:86	Enzymatic, urease-mediated mineralization of gellan gum hydrogel with calcium carbonate, magnesium-enriched calcium carbonate and magnesium carbonate for bone regeneration applications.
28569438	0	72	theme	calcium	108:114	arg1	carbonate					116:124	magnesium-enriched calcium carbonate	89:124	magnesium-enriched calcium carbonate	89:124	Enzymatic, urease-mediated mineralization of gellan gum hydrogel with calcium carbonate, magnesium-enriched calcium carbonate and magnesium carbonate for bone regeneration applications.
28569438	5	73	theme	different	1025:1033	arg1	ratios					1035:1040	different ratios	1025:1040	different ratios of calcium and magnesium ions	1025:1070	Hydrogels loaded with the enzyme urease were mineralized by incubation in mineralization media containing urea and different ratios of calcium and magnesium ions.
28569438	12	74	theme	mineralized	1743:1753	arg1	Hydrogels					1733:1741	Hydrogels	1733:1741	Hydrogels mineralized with hydromagnesite	1733:1773	Hydrogels mineralized with hydromagnesite displayed higher cytotoxicity.
28569438	13	75	theme	magnesium	1989:1997	arg1	enrichment					1999:2008	magnesium enrichment	1989:2008	magnesium enrichment	1989:2008	In conclusion, enzymatic mineralization of GG hydrogels with CaCO3 in the form of calcite successfully reinforced hydrogels and promoted osteoblast-like cell adhesion and growth, but magnesium enrichment had no definitive positive effect.
28569438	4	76	from	mineralized	771:781	arg1	study					733:737	the present study	721:737	the present study	721:737	In the present study, gellan gum (GG) hydrogels were mineralized enzymatically with CaCO3 , Mg-enriched CaCO3 and magnesium carbonate to generate composite biomaterials for bone regeneration.
28569438	10	77	theme	elemental	1522:1530	arg1	ratio					1532:1536	The calcium:magnesium elemental ratio	1500:1536	The calcium:magnesium elemental ratio in the mineral formed	1500:1558	The calcium:magnesium elemental ratio in the mineral formed was higher than in the respective mineralization media.
28569438	10	77	theme	elemental	1522:1530	arg1	higher					1564:1569	higher	1564:1569	higher	1564:1569	The calcium:magnesium elemental ratio in the mineral formed was higher than in the respective mineralization media.
28569438	13	78	theme	cell	1959:1962	arg1	adhesion					1964:1971	osteoblast-like cell adhesion	1943:1971	osteoblast-like cell adhesion	1943:1971	In conclusion, enzymatic mineralization of GG hydrogels with CaCO3 in the form of calcite successfully reinforced hydrogels and promoted osteoblast-like cell adhesion and growth, but magnesium enrichment had no definitive positive effect.
28569438	2	79	theme	hydrogel-CaCO3	409:422	arg1	composites					424:433	hydrogel-CaCO3 composites	409:433	hydrogel-CaCO3 composites	409:433	Calcium carbonate (CaCO3 ) has been successfully applied as a bone regeneration material, but hydrogel-CaCO3 composites have received less attention.
28569438	5	80	from	incubation	970:979	arg1	media					999:1003	mineralization media	984:1003	mineralization media containing urea	984:1019	Hydrogels loaded with the enzyme urease were mineralized by incubation in mineralization media containing urea and different ratios of calcium and magnesium ions.
28569438	3	81	theme	biomaterials	681:692	arg1	component					652:660	a component	650:660	a component of carbonate-based biomaterials	650:692	Magnesium (Mg) has been used as a component of calcium phosphate biomaterials to stimulate bone-forming cell adhesion and proliferation and bone regeneration in vivo, but its effect as a component of carbonate-based biomaterials remains uninvestigated.
28569438	0	82	theme	gellan	45:50	arg1	hydrogel					56:63	gellan gum hydrogel	45:63	gellan gum hydrogel	45:63	Enzymatic, urease-mediated mineralization of gellan gum hydrogel with calcium carbonate, magnesium-enriched calcium carbonate and magnesium carbonate for bone regeneration applications.
28569438	6	83	theme	mineral	1122:1128	arg1	crystallinity					1130:1142	mineral crystallinity	1122:1142	mineral crystallinity	1122:1142	Increasing the magnesium concentration decreased mineral crystallinity.
28569438	0	84	with	mineralization	27:40	arg1	carbonate					78:86	calcium carbonate	70:86	calcium carbonate	70:86	Enzymatic, urease-mediated mineralization of gellan gum hydrogel with calcium carbonate, magnesium-enriched calcium carbonate and magnesium carbonate for bone regeneration applications.
28569438	0	84	with	mineralization	27:40	arg1	carbonate					116:124	magnesium-enriched calcium carbonate	89:124	magnesium-enriched calcium carbonate	89:124	Enzymatic, urease-mediated mineralization of gellan gum hydrogel with calcium carbonate, magnesium-enriched calcium carbonate and magnesium carbonate for bone regeneration applications.
28569438	0	84	with	mineralization	27:40	arg1	carbonate					140:148	magnesium carbonate	130:148	magnesium carbonate for bone regeneration applications	130:183	Enzymatic, urease-mediated mineralization of gellan gum hydrogel with calcium carbonate, magnesium-enriched calcium carbonate and magnesium carbonate for bone regeneration applications.
28569438	0	85	theme	hydrogel	56:63	arg1	mineralization					27:40	Enzymatic, urease-mediated mineralization	0:40	Enzymatic, urease-mediated mineralization of gellan gum hydrogel with calcium carbonate, magnesium-enriched calcium carbonate and magnesium carbonate for bone regeneration applications.	0:184	Enzymatic, urease-mediated mineralization of gellan gum hydrogel with calcium carbonate, magnesium-enriched calcium carbonate and magnesium carbonate for bone regeneration applications.
28569438	9	86	theme	strength	1411:1418	arg1	strength					1411:1418	mineral formed and compressive strength	1380:1418	mineral formed and compressive strength	1380:1418	The amount of mineral formed and compressive strength decreased with increasing magnesium concentration in the mineralization medium.
28569438	9	86	theme	strength	1411:1418	arg1	amount					1370:1375	The amount	1366:1375	The amount of mineral formed and compressive strength	1366:1418	The amount of mineral formed and compressive strength decreased with increasing magnesium concentration in the mineralization medium.
28569438	4	87	theme	composite	864:872	arg1	biomaterials					874:885	composite biomaterials	864:885	composite biomaterials for bone regeneration	864:907	In the present study, gellan gum (GG) hydrogels were mineralized enzymatically with CaCO3 , Mg-enriched CaCO3 and magnesium carbonate to generate composite biomaterials for bone regeneration.
28569438	3	88	theme	phosphate	520:528	arg1	biomaterials					530:541	calcium phosphate biomaterials	512:541	calcium phosphate biomaterials	512:541	Magnesium (Mg) has been used as a component of calcium phosphate biomaterials to stimulate bone-forming cell adhesion and proliferation and bone regeneration in vivo, but its effect as a component of carbonate-based biomaterials remains uninvestigated.
28569438	9	89	theme	magnesium	1446:1454	arg1	concentration					1456:1468	magnesium concentration	1446:1468	magnesium concentration	1446:1468	The amount of mineral formed and compressive strength decreased with increasing magnesium concentration in the mineralization medium.
28569438	5	90	theme	ions	1067:1070	arg1	incubation					970:979	incubation	970:979	incubation in mineralization media containing urea	970:1019	Hydrogels loaded with the enzyme urease were mineralized by incubation in mineralization media containing urea and different ratios of calcium and magnesium ions.
28569438	5	90	theme	ions	1067:1070	arg1	ratios					1035:1040	different ratios	1025:1040	different ratios of calcium and magnesium ions	1025:1070	Hydrogels loaded with the enzyme urease were mineralized by incubation in mineralization media containing urea and different ratios of calcium and magnesium ions.
28569438	4	91	theme	magnesium	832:840	arg1	carbonate					842:850	magnesium carbonate	832:850	magnesium carbonate	832:850	In the present study, gellan gum (GG) hydrogels were mineralized enzymatically with CaCO3 , Mg-enriched CaCO3 and magnesium carbonate to generate composite biomaterials for bone regeneration.
28569438	3	92	theme	bone-forming	556:567	arg1	adhesion					574:581	bone-forming cell adhesion	556:581	bone-forming cell adhesion	556:581	Magnesium (Mg) has been used as a component of calcium phosphate biomaterials to stimulate bone-forming cell adhesion and proliferation and bone regeneration in vivo, but its effect as a component of carbonate-based biomaterials remains uninvestigated.
26835987	2	0	theme	exact	349:353	arg1	unclear					410:416	unclear	410:416	unclear	410:416	At present, the exact molecular mechanism of ESP-B4 on treating arthritis is unclear.
26835987	2	0	theme	exact	349:353	arg1	mechanism					365:373	the exact molecular mechanism	345:373	the exact molecular mechanism of ESP-B4 on treating arthritis	345:405	At present, the exact molecular mechanism of ESP-B4 on treating arthritis is unclear.
26835987	0	1	theme	inhibiting	68:77	arg1	expression					89:98	arthritis and inhibiting cytokines expression	54:98	arthritis and inhibiting cytokines expression	54:98	A pure polysaccharide from Ephedra sinica treating on arthritis and inhibiting cytokines expression.
26835987	3	2	from	cells	502:506	arg1	rats					549:552	Wistar rats	542:552	Wistar rats	542:552	We are thus evaluating the properties of ESP-B4 on LPS-induced THP-1 pro-monocytic cells and adjuvant-induced arthritis in Wistar rats via TLR4.
26835987	3	2	from	cells	502:506	arg1	properties					446:455	the properties	442:455	the properties of ESP-B4 on LPS-induced THP-1 pro-monocytic cells and adjuvant-induced arthritis in Wistar rats	442:552	We are thus evaluating the properties of ESP-B4 on LPS-induced THP-1 pro-monocytic cells and adjuvant-induced arthritis in Wistar rats via TLR4.
26835987	1	3	theme	acidic	142:147	arg1	polysaccharides					149:163	the acidic polysaccharides	138:163	the acidic polysaccharides of Ephedra sinica	138:181	In our previous study, we found that the acidic polysaccharides of Ephedra sinica had immunosuppressive effect to treat rheumatoid arthritis and the pure polysaccharide ESP-B4 was the main composition of the acidic polysaccharides.
26835987	7	4	theme	disease	867:873	arg1	days					859:862	12 days	856:862	12 days of disease induced by adjuvant	856:893	Furthermore, in vivo, after 12 days of disease induced by adjuvant, rats were treated with ESP-B4 for 16 days.
26835987	1	5	theme	pure	250:253	arg1	composition					290:300	the main composition	281:300	the main composition of the acidic polysaccharides	281:330	In our previous study, we found that the acidic polysaccharides of Ephedra sinica had immunosuppressive effect to treat rheumatoid arthritis and the pure polysaccharide ESP-B4 was the main composition of the acidic polysaccharides.
26835987	1	5	theme	pure	250:253	arg1	ESP-B4					270:275	the pure polysaccharide ESP-B4	246:275	the pure polysaccharide ESP-B4	246:275	In our previous study, we found that the acidic polysaccharides of Ephedra sinica had immunosuppressive effect to treat rheumatoid arthritis and the pure polysaccharide ESP-B4 was the main composition of the acidic polysaccharides.
26835987	5	6	theme	NF-κB	722:726	arg1	subunit					711:717	p65 subunit	707:717	p65 subunit of NF-κB	707:726	In addition, ESP-B4 reduced the LPS-stimulated nuclear translocation of p65 subunit of NF-κB.
26835987	8	7	theme	inflammation	987:998	arg1	parameters					973:982	all parameters	969:982	all parameters of inflammation	969:998	ESP-B4 significantly improved all parameters of inflammation.
26835987	1	8	theme	polysaccharide	255:268	arg1	composition					290:300	the main composition	281:300	the main composition of the acidic polysaccharides	281:330	In our previous study, we found that the acidic polysaccharides of Ephedra sinica had immunosuppressive effect to treat rheumatoid arthritis and the pure polysaccharide ESP-B4 was the main composition of the acidic polysaccharides.
26835987	1	8	theme	polysaccharide	255:268	arg1	ESP-B4					270:275	the pure polysaccharide ESP-B4	246:275	the pure polysaccharide ESP-B4	246:275	In our previous study, we found that the acidic polysaccharides of Ephedra sinica had immunosuppressive effect to treat rheumatoid arthritis and the pure polysaccharide ESP-B4 was the main composition of the acidic polysaccharides.
26835987	3	9	theme	THP-1	482:486	arg1	cells					502:506	LPS-induced THP-1 pro-monocytic cells	470:506	LPS-induced THP-1 pro-monocytic cells	470:506	We are thus evaluating the properties of ESP-B4 on LPS-induced THP-1 pro-monocytic cells and adjuvant-induced arthritis in Wistar rats via TLR4.
26835987	2	10	from	present	336:342	arg1	unclear					410:416	unclear	410:416	unclear	410:416	At present, the exact molecular mechanism of ESP-B4 on treating arthritis is unclear.
26835987	2	10	from	present	336:342	arg1	mechanism					365:373	the exact molecular mechanism	345:373	the exact molecular mechanism of ESP-B4 on treating arthritis	345:405	At present, the exact molecular mechanism of ESP-B4 on treating arthritis is unclear.
26835987	1	11	theme	Ephedra	168:174	arg1	sinica					176:181	Ephedra sinica	168:181	Ephedra sinica	168:181	In our previous study, we found that the acidic polysaccharides of Ephedra sinica had immunosuppressive effect to treat rheumatoid arthritis and the pure polysaccharide ESP-B4 was the main composition of the acidic polysaccharides.
26835987	6	12	with	Pretreatment	729:740	arg1	ESP-B4					747:752	ESP-B4	747:752	ESP-B4	747:752	Pretreatment with ESP-B4 significantly down-regulated the phosphorylation of MAPKs induced by LPS.
26835987	0	13	from	sinica	35:40	arg1	polysaccharide					7:20	A pure polysaccharide	0:20	A pure polysaccharide from Ephedra sinica	0:40	A pure polysaccharide from Ephedra sinica treating on arthritis and inhibiting cytokines expression.
26835987	3	14	theme	Wistar	542:547	arg1	rats					549:552	Wistar rats	542:552	Wistar rats	542:552	We are thus evaluating the properties of ESP-B4 on LPS-induced THP-1 pro-monocytic cells and adjuvant-induced arthritis in Wistar rats via TLR4.
26835987	9	15	theme	TLR4	1084:1087	arg1	pathway					1099:1105	the TLR4 signaling pathway	1080:1105	the TLR4 signaling pathway to treat rheumatoid arthritis	1080:1135	ESP-B4 reduced the release of inflammatory factors and cytokines by inhibiting the TLR4 signaling pathway to treat rheumatoid arthritis.
26835987	0	16	theme	pure	2:5	arg1	polysaccharide					7:20	A pure polysaccharide	0:20	A pure polysaccharide from Ephedra sinica	0:40	A pure polysaccharide from Ephedra sinica treating on arthritis and inhibiting cytokines expression.
26835987	6	17	theme	MAPKs	806:810	arg1	phosphorylation					787:801	the phosphorylation	783:801	the phosphorylation of MAPKs induced by LPS	783:825	Pretreatment with ESP-B4 significantly down-regulated the phosphorylation of MAPKs induced by LPS.
26835987	9	18	theme	signaling	1089:1097	arg1	pathway					1099:1105	the TLR4 signaling pathway	1080:1105	the TLR4 signaling pathway to treat rheumatoid arthritis	1080:1135	ESP-B4 reduced the release of inflammatory factors and cytokines by inhibiting the TLR4 signaling pathway to treat rheumatoid arthritis.
26835987	2	19	theme	ESP-B4	378:383	arg1	unclear					410:416	unclear	410:416	unclear	410:416	At present, the exact molecular mechanism of ESP-B4 on treating arthritis is unclear.
26835987	2	19	theme	ESP-B4	378:383	arg1	mechanism					365:373	the exact molecular mechanism	345:373	the exact molecular mechanism of ESP-B4 on treating arthritis	345:405	At present, the exact molecular mechanism of ESP-B4 on treating arthritis is unclear.
26835987	1	20	theme	sinica	176:181	arg1	polysaccharides					149:163	the acidic polysaccharides	138:163	the acidic polysaccharides of Ephedra sinica	138:181	In our previous study, we found that the acidic polysaccharides of Ephedra sinica had immunosuppressive effect to treat rheumatoid arthritis and the pure polysaccharide ESP-B4 was the main composition of the acidic polysaccharides.
26835987	3	21	theme	ESP-B4	460:465	arg1	properties					446:455	the properties	442:455	the properties of ESP-B4 on LPS-induced THP-1 pro-monocytic cells and adjuvant-induced arthritis in Wistar rats	442:552	We are thus evaluating the properties of ESP-B4 on LPS-induced THP-1 pro-monocytic cells and adjuvant-induced arthritis in Wistar rats via TLR4.
26835987	3	22	from	arthritis	529:537	arg1	rats					549:552	Wistar rats	542:552	Wistar rats	542:552	We are thus evaluating the properties of ESP-B4 on LPS-induced THP-1 pro-monocytic cells and adjuvant-induced arthritis in Wistar rats via TLR4.
26835987	3	22	from	arthritis	529:537	arg1	properties					446:455	the properties	442:455	the properties of ESP-B4 on LPS-induced THP-1 pro-monocytic cells and adjuvant-induced arthritis in Wistar rats	442:552	We are thus evaluating the properties of ESP-B4 on LPS-induced THP-1 pro-monocytic cells and adjuvant-induced arthritis in Wistar rats via TLR4.
26835987	1	23	theme	main	285:288	arg1	composition					290:300	the main composition	281:300	the main composition of the acidic polysaccharides	281:330	In our previous study, we found that the acidic polysaccharides of Ephedra sinica had immunosuppressive effect to treat rheumatoid arthritis and the pure polysaccharide ESP-B4 was the main composition of the acidic polysaccharides.
26835987	1	23	theme	main	285:288	arg1	ESP-B4					270:275	the pure polysaccharide ESP-B4	246:275	the pure polysaccharide ESP-B4	246:275	In our previous study, we found that the acidic polysaccharides of Ephedra sinica had immunosuppressive effect to treat rheumatoid arthritis and the pure polysaccharide ESP-B4 was the main composition of the acidic polysaccharides.
26835987	5	24	theme	p65	707:709	arg1	subunit					711:717	p65 subunit	707:717	p65 subunit of NF-κB	707:726	In addition, ESP-B4 reduced the LPS-stimulated nuclear translocation of p65 subunit of NF-κB.
26835987	0	25	dep	arthritis	54:62	arg1	cytokines					79:87	cytokines	79:87	cytokines	79:87	A pure polysaccharide from Ephedra sinica treating on arthritis and inhibiting cytokines expression.
26835987	1	26	theme	immunosuppressive	187:203	arg1	effect					205:210	immunosuppressive effect	187:210	immunosuppressive effect	187:210	In our previous study, we found that the acidic polysaccharides of Ephedra sinica had immunosuppressive effect to treat rheumatoid arthritis and the pure polysaccharide ESP-B4 was the main composition of the acidic polysaccharides.
26835987	5	27	theme	subunit	711:717	arg1	translocation					690:702	the LPS-stimulated nuclear translocation	663:702	the LPS-stimulated nuclear translocation of p65 subunit of NF-κB	663:726	In addition, ESP-B4 reduced the LPS-stimulated nuclear translocation of p65 subunit of NF-κB.
26835987	9	28	theme	cytokines	1056:1064	arg1	release					1020:1026	the release	1016:1026	the release of inflammatory factors and cytokines	1016:1064	ESP-B4 reduced the release of inflammatory factors and cytokines by inhibiting the TLR4 signaling pathway to treat rheumatoid arthritis.
26835987	9	29	theme	rheumatoid	1116:1125	arg1	arthritis					1127:1135	rheumatoid arthritis	1116:1135	rheumatoid arthritis	1116:1135	ESP-B4 reduced the release of inflammatory factors and cytokines by inhibiting the TLR4 signaling pathway to treat rheumatoid arthritis.
26835987	0	30	theme	Ephedra	27:33	arg1	sinica					35:40	Ephedra sinica	27:40	Ephedra sinica	27:40	A pure polysaccharide from Ephedra sinica treating on arthritis and inhibiting cytokines expression.
26835987	3	31	from	properties	446:455	arg1	cells					502:506	LPS-induced THP-1 pro-monocytic cells	470:506	LPS-induced THP-1 pro-monocytic cells	470:506	We are thus evaluating the properties of ESP-B4 on LPS-induced THP-1 pro-monocytic cells and adjuvant-induced arthritis in Wistar rats via TLR4.
26835987	3	31	from	properties	446:455	arg1	arthritis					529:537	adjuvant-induced arthritis	512:537	adjuvant-induced arthritis in Wistar rats	512:552	We are thus evaluating the properties of ESP-B4 on LPS-induced THP-1 pro-monocytic cells and adjuvant-induced arthritis in Wistar rats via TLR4.
26835987	1	32	theme	previous	108:115	arg1	study					117:121	our previous study	104:121	our previous study	104:121	In our previous study, we found that the acidic polysaccharides of Ephedra sinica had immunosuppressive effect to treat rheumatoid arthritis and the pure polysaccharide ESP-B4 was the main composition of the acidic polysaccharides.
26835987	1	33	theme	acidic	309:314	arg1	polysaccharides					316:330	the acidic polysaccharides	305:330	the acidic polysaccharides	305:330	In our previous study, we found that the acidic polysaccharides of Ephedra sinica had immunosuppressive effect to treat rheumatoid arthritis and the pure polysaccharide ESP-B4 was the main composition of the acidic polysaccharides.
26835987	5	34	theme	LPS-stimulated	667:680	arg1	translocation					690:702	the LPS-stimulated nuclear translocation	663:702	the LPS-stimulated nuclear translocation of p65 subunit of NF-κB	663:726	In addition, ESP-B4 reduced the LPS-stimulated nuclear translocation of p65 subunit of NF-κB.
26835987	3	35	theme	pro-monocytic	488:500	arg1	cells					502:506	LPS-induced THP-1 pro-monocytic cells	470:506	LPS-induced THP-1 pro-monocytic cells	470:506	We are thus evaluating the properties of ESP-B4 on LPS-induced THP-1 pro-monocytic cells and adjuvant-induced arthritis in Wistar rats via TLR4.
26835987	1	36	theme	polysaccharides	316:330	arg1	composition					290:300	the main composition	281:300	the main composition of the acidic polysaccharides	281:330	In our previous study, we found that the acidic polysaccharides of Ephedra sinica had immunosuppressive effect to treat rheumatoid arthritis and the pure polysaccharide ESP-B4 was the main composition of the acidic polysaccharides.
26835987	1	36	theme	polysaccharides	316:330	arg1	ESP-B4					270:275	the pure polysaccharide ESP-B4	246:275	the pure polysaccharide ESP-B4	246:275	In our previous study, we found that the acidic polysaccharides of Ephedra sinica had immunosuppressive effect to treat rheumatoid arthritis and the pure polysaccharide ESP-B4 was the main composition of the acidic polysaccharides.
26835987	5	37	theme	nuclear	682:688	arg1	translocation					690:702	the LPS-stimulated nuclear translocation	663:702	the LPS-stimulated nuclear translocation of p65 subunit of NF-κB	663:726	In addition, ESP-B4 reduced the LPS-stimulated nuclear translocation of p65 subunit of NF-κB.
26835987	3	38	from	ESP-B4	460:465	arg1	cells					502:506	LPS-induced THP-1 pro-monocytic cells	470:506	LPS-induced THP-1 pro-monocytic cells	470:506	We are thus evaluating the properties of ESP-B4 on LPS-induced THP-1 pro-monocytic cells and adjuvant-induced arthritis in Wistar rats via TLR4.
26835987	3	38	from	ESP-B4	460:465	arg1	arthritis					529:537	adjuvant-induced arthritis	512:537	adjuvant-induced arthritis in Wistar rats	512:552	We are thus evaluating the properties of ESP-B4 on LPS-induced THP-1 pro-monocytic cells and adjuvant-induced arthritis in Wistar rats via TLR4.
26835987	3	39	theme	LPS-induced	470:480	arg1	cells					502:506	LPS-induced THP-1 pro-monocytic cells	470:506	LPS-induced THP-1 pro-monocytic cells	470:506	We are thus evaluating the properties of ESP-B4 on LPS-induced THP-1 pro-monocytic cells and adjuvant-induced arthritis in Wistar rats via TLR4.
26835987	1	40	theme	rheumatoid	221:230	arg1	arthritis					232:240	rheumatoid arthritis	221:240	rheumatoid arthritis	221:240	In our previous study, we found that the acidic polysaccharides of Ephedra sinica had immunosuppressive effect to treat rheumatoid arthritis and the pure polysaccharide ESP-B4 was the main composition of the acidic polysaccharides.
26835987	1	41	contain	had	183:185	arg1	polysaccharides					149:163	the acidic polysaccharides	138:163	the acidic polysaccharides of Ephedra sinica	138:181	In our previous study, we found that the acidic polysaccharides of Ephedra sinica had immunosuppressive effect to treat rheumatoid arthritis and the pure polysaccharide ESP-B4 was the main composition of the acidic polysaccharides.
26835987	1	41	contain	had	183:185	arg2	effect					205:210	immunosuppressive effect	187:210	immunosuppressive effect	187:210	In our previous study, we found that the acidic polysaccharides of Ephedra sinica had immunosuppressive effect to treat rheumatoid arthritis and the pure polysaccharide ESP-B4 was the main composition of the acidic polysaccharides.
26835987	3	42	theme	adjuvant-induced	512:527	arg1	arthritis					529:537	adjuvant-induced arthritis	512:537	adjuvant-induced arthritis in Wistar rats	512:552	We are thus evaluating the properties of ESP-B4 on LPS-induced THP-1 pro-monocytic cells and adjuvant-induced arthritis in Wistar rats via TLR4.
26835987	9	43	theme	inflammatory	1031:1042	arg1	factors					1044:1050	inflammatory factors	1031:1050	inflammatory factors	1031:1050	ESP-B4 reduced the release of inflammatory factors and cytokines by inhibiting the TLR4 signaling pathway to treat rheumatoid arthritis.
26835987	0	44	theme	arthritis	54:62	arg1	expression					89:98	arthritis and inhibiting cytokines expression	54:98	arthritis and inhibiting cytokines expression	54:98	A pure polysaccharide from Ephedra sinica treating on arthritis and inhibiting cytokines expression.
26835987	2	45	theme	molecular	355:363	arg1	unclear					410:416	unclear	410:416	unclear	410:416	At present, the exact molecular mechanism of ESP-B4 on treating arthritis is unclear.
26835987	2	45	theme	molecular	355:363	arg1	mechanism					365:373	the exact molecular mechanism	345:373	the exact molecular mechanism of ESP-B4 on treating arthritis	345:405	At present, the exact molecular mechanism of ESP-B4 on treating arthritis is unclear.
26835987	9	46	theme	factors	1044:1050	arg1	release					1020:1026	the release	1016:1026	the release of inflammatory factors and cytokines	1016:1064	ESP-B4 reduced the release of inflammatory factors and cytokines by inhibiting the TLR4 signaling pathway to treat rheumatoid arthritis.
26835987	4	47	theme	cytokines	609:617	arg1	production					595:604	the production	591:604	the production of cytokines induced by LPS	591:632	In vitro, ESP-B4 decreased the production of cytokines induced by LPS.
29146537	5	0	theme	uniform	821:827	arg1	distribution					834:845	no uniform heat distribution	818:845	no uniform heat distribution	818:845	The qualification of the continuously operating coating machine reveals no uniform heat distribution during drying.
29146537	6	1	theme	film	968:971	arg1	thickness					973:981	wet film thickness	964:981	wet film thickness of 500 μm, velocity of 15.9 cm/min	964:1016	Coating solutions for continuous manufacturing should provide at least a dynamic viscosity of 1 Pa*s (wet film thickness of 500 μm, velocity of 15.9 cm/min).
29146537	8	2	theme	Continuous	1128:1137	arg1	production					1139:1148	Continuous production	1128:1148	Continuous production	1128:1148	Continuous production lead to lower drug content of the films.
29146537	3	3	from	effects	458:464	arg1	films					480:484	the formed films	469:484	the formed films	469:484	In this study, a continuous coating machine was qualified in regards of the process conditions for film compositions and their effects on the formed films.
29146537	1	4	theme	special	159:165	arg1	populations					175:185	special patient populations	159:185	special patient populations	159:185	Orodispersible films have been described as new beneficial dosage forms for special patient populations.
29146537	10	5	theme	process	1316:1322	arg1	effects					1290:1296	observed significant effects	1269:1296	observed significant effects of the production process on the film characteristics	1269:1350	There are observed significant effects of the production process on the film characteristics.
29146537	5	6	theme	heat	829:832	arg1	distribution					834:845	no uniform heat distribution	818:845	no uniform heat distribution	818:845	The qualification of the continuously operating coating machine reveals no uniform heat distribution during drying.
29146537	6	7	theme	wet	964:966	arg1	thickness					973:981	wet film thickness	964:981	wet film thickness of 500 μm, velocity of 15.9 cm/min	964:1016	Coating solutions for continuous manufacturing should provide at least a dynamic viscosity of 1 Pa*s (wet film thickness of 500 μm, velocity of 15.9 cm/min).
29146537	6	8	theme	continuous	884:893	arg1	manufacturing					895:907	continuous manufacturing	884:907	continuous manufacturing	884:907	Coating solutions for continuous manufacturing should provide at least a dynamic viscosity of 1 Pa*s (wet film thickness of 500 μm, velocity of 15.9 cm/min).
29146537	1	9	theme	patient	167:173	arg1	populations					175:185	special patient populations	159:185	special patient populations	159:185	Orodispersible films have been described as new beneficial dosage forms for special patient populations.
29146537	10	10	theme	production	1305:1314	arg1	process					1316:1322	the production process	1301:1322	the production process	1301:1322	There are observed significant effects of the production process on the film characteristics.
29146537	4	11	theme	manufacturing	527:539	arg1	processes					541:549	both manufacturing processes	522:549	both manufacturing processes	522:549	To investigate differences between both manufacturing processes, various film formulations of hydrochlorothiazide and hydroxypropylcellulose (HPC) or hydroxypropylmethycellulose (HPMC) as film formers were produced and the resulting films were characterized.
29146537	2	12	theme	continuous	305:314	arg1	manufacturing					316:328	non- continuous and continuous manufacturing	285:328	non- continuous and continuous manufacturing	285:328	Due to various production settings, different requirements on film formulations are required for non- continuous and continuous manufacturing.
29146537	9	13	theme	films	1217:1221	arg1	disintegrate					1223:1234	films disintegrate	1217:1234	films disintegrate	1217:1234	All continuously produced films disintegrate within less than 30 s.
29146537	5	14	theme	operating	784:792	arg1	machine					802:808	the continuously operating coating machine	767:808	the continuously operating coating machine	767:808	The qualification of the continuously operating coating machine reveals no uniform heat distribution during drying.
29146537	6	15	theme	s	961:961	arg1	viscosity					943:951	at least a dynamic viscosity	924:951	at least a dynamic viscosity of 1 Pa*s (wet film thickness of 500 μm, velocity of 15.9 cm/min)	924:1017	Coating solutions for continuous manufacturing should provide at least a dynamic viscosity of 1 Pa*s (wet film thickness of 500 μm, velocity of 15.9 cm/min).
29146537	2	16	from	requirements	234:245	arg1	formulations					255:266	film formulations	250:266	film formulations	250:266	Due to various production settings, different requirements on film formulations are required for non- continuous and continuous manufacturing.
29146537	3	17	theme	coating	359:365	arg1	machine					367:373	a continuous coating machine	346:373	a continuous coating machine	346:373	In this study, a continuous coating machine was qualified in regards of the process conditions for film compositions and their effects on the formed films.
29146537	0	18	from	lab-scale	44:52	arg1	transfer					30:37	Product transfer	22:37	Product transfer from lab-scale to continuous manufacturing	22:80	Orodispersible films: Product transfer from lab-scale to continuous manufacturing.
29146537	11	19	theme	film	1371:1374	arg1	manufacturing					1376:1388	film manufacturing	1371:1388	film manufacturing	1371:1388	When transferring film manufacturing from lab-scale to continuous mode, film compositions, processing conditions and suitable characterization methods have to be carefully selected and adopted.
29146537	6	20	theme	Coating	862:868	arg1	solutions					870:878	Coating solutions	862:878	Coating solutions for continuous manufacturing	862:907	Coating solutions for continuous manufacturing should provide at least a dynamic viscosity of 1 Pa*s (wet film thickness of 500 μm, velocity of 15.9 cm/min).
29146537	2	21	theme	continuous	290:299	arg1	manufacturing					316:328	non- continuous and continuous manufacturing	285:328	non- continuous and continuous manufacturing	285:328	Due to various production settings, different requirements on film formulations are required for non- continuous and continuous manufacturing.
29146537	10	22	from	effects	1290:1296	arg1	characteristics					1336:1350	the film characteristics	1327:1350	the film characteristics	1327:1350	There are observed significant effects of the production process on the film characteristics.
29146537	5	23	theme	machine	802:808	arg1	qualification					750:762	The qualification	746:762	The qualification of the continuously operating coating machine	746:808	The qualification of the continuously operating coating machine reveals no uniform heat distribution during drying.
29146537	6	24	theme	1 Pa	956:959	arg1	s					961:961	1 Pa*s	956:961	1 Pa*s (wet film thickness of 500 μm, velocity of 15.9 cm/min)	956:1017	Coating solutions for continuous manufacturing should provide at least a dynamic viscosity of 1 Pa*s (wet film thickness of 500 μm, velocity of 15.9 cm/min).
29146537	2	25	theme	non-	285:288	arg1	manufacturing					316:328	non- continuous and continuous manufacturing	285:328	non- continuous and continuous manufacturing	285:328	Due to various production settings, different requirements on film formulations are required for non- continuous and continuous manufacturing.
29146537	10	26	theme	film	1331:1334	arg1	characteristics					1336:1350	the film characteristics	1327:1350	the film characteristics	1327:1350	There are observed significant effects of the production process on the film characteristics.
29146537	5	27	theme	coating	794:800	arg1	machine					802:808	the continuously operating coating machine	767:808	the continuously operating coating machine	767:808	The qualification of the continuously operating coating machine reveals no uniform heat distribution during drying.
29146537	7	28	contain	contain	1030:1036	arg1	mode					1122:1125	continuous production mode	1100:1125	continuous production mode	1100:1125	HPC films contain higher residuals of ethanol or acetone in bench-scale than in continuous production mode.
29146537	7	28	contain	contain	1030:1036	arg1	films					1024:1028	HPC films	1020:1028	HPC films	1020:1028	HPC films contain higher residuals of ethanol or acetone in bench-scale than in continuous production mode.
29146537	7	28	contain	contain	1030:1036	arg2	films					1024:1028	HPC films	1020:1028	HPC films	1020:1028	HPC films contain higher residuals of ethanol or acetone in bench-scale than in continuous production mode.
29146537	7	28	contain	contain	1030:1036	arg2	residuals					1045:1053	higher residuals	1038:1053	higher residuals of ethanol or acetone in bench-scale	1038:1090	HPC films contain higher residuals of ethanol or acetone in bench-scale than in continuous production mode.
29146537	6	29	theme	*	960:960	arg1	s					961:961	1 Pa*s	956:961	1 Pa*s (wet film thickness of 500 μm, velocity of 15.9 cm/min)	956:1017	Coating solutions for continuous manufacturing should provide at least a dynamic viscosity of 1 Pa*s (wet film thickness of 500 μm, velocity of 15.9 cm/min).
29146537	4	30	theme	resulting	710:718	arg1	films					720:724	the resulting films	706:724	the resulting films	706:724	To investigate differences between both manufacturing processes, various film formulations of hydrochlorothiazide and hydroxypropylcellulose (HPC) or hydroxypropylmethycellulose (HPMC) as film formers were produced and the resulting films were characterized.
29146537	0	31	theme	Orodispersible	0:13	arg1	films					15:19	Orodispersible films	0:19	Orodispersible films	0:19	Orodispersible films: Product transfer from lab-scale to continuous manufacturing.
29146537	4	32	theme	hydrochlorothiazide	581:599	arg1	formulations					565:576	various film formulations	552:576	various film formulations of hydrochlorothiazide and hydroxypropylcellulose (HPC) or hydroxypropylmethycellulose (HPMC) as film formers	552:686	To investigate differences between both manufacturing processes, various film formulations of hydrochlorothiazide and hydroxypropylcellulose (HPC) or hydroxypropylmethycellulose (HPMC) as film formers were produced and the resulting films were characterized.
29146537	11	33	theme	continuous	1408:1417	arg1	mode					1419:1422	continuous mode	1408:1422	continuous mode	1408:1422	When transferring film manufacturing from lab-scale to continuous mode, film compositions, processing conditions and suitable characterization methods have to be carefully selected and adopted.
29146537	11	34	theme	suitable	1470:1477	arg1	methods					1496:1502	suitable characterization methods	1470:1502	suitable characterization methods	1470:1502	When transferring film manufacturing from lab-scale to continuous mode, film compositions, processing conditions and suitable characterization methods have to be carefully selected and adopted.
29146537	7	35	from	residuals	1045:1053	arg1	bench-scale					1080:1090	bench-scale	1080:1090	bench-scale	1080:1090	HPC films contain higher residuals of ethanol or acetone in bench-scale than in continuous production mode.
29146537	0	36	theme	Product	22:28	arg1	transfer					30:37	Product transfer	22:37	Product transfer from lab-scale to continuous manufacturing	22:80	Orodispersible films: Product transfer from lab-scale to continuous manufacturing.
29146537	6	37	theme	15.9 cm/min	1006:1016	arg1	velocity					994:1001	velocity	994:1001	velocity of 15.9 cm/min	994:1016	Coating solutions for continuous manufacturing should provide at least a dynamic viscosity of 1 Pa*s (wet film thickness of 500 μm, velocity of 15.9 cm/min).
29146537	6	37	theme	15.9 cm/min	1006:1016	arg1	500 μm					986:991	500 μm	986:991	500 μm	986:991	Coating solutions for continuous manufacturing should provide at least a dynamic viscosity of 1 Pa*s (wet film thickness of 500 μm, velocity of 15.9 cm/min).
29146537	0	38	dep	transfer	30:37	arg1	films					15:19	Orodispersible films	0:19	Orodispersible films	0:19	Orodispersible films: Product transfer from lab-scale to continuous manufacturing.
29146537	3	39	theme	formed	473:478	arg1	films					480:484	the formed films	469:484	the formed films	469:484	In this study, a continuous coating machine was qualified in regards of the process conditions for film compositions and their effects on the formed films.
29146537	7	40	theme	higher	1038:1043	arg1	residuals					1045:1053	higher residuals	1038:1053	higher residuals of ethanol or acetone in bench-scale	1038:1090	HPC films contain higher residuals of ethanol or acetone in bench-scale than in continuous production mode.
29146537	4	41	theme	hydroxypropylcellulose	605:626	arg1	formulations					565:576	various film formulations	552:576	various film formulations of hydrochlorothiazide and hydroxypropylcellulose (HPC) or hydroxypropylmethycellulose (HPMC) as film formers	552:686	To investigate differences between both manufacturing processes, various film formulations of hydrochlorothiazide and hydroxypropylcellulose (HPC) or hydroxypropylmethycellulose (HPMC) as film formers were produced and the resulting films were characterized.
29146537	11	42	theme	film	1425:1428	arg1	compositions					1430:1441	film compositions	1425:1441	film compositions	1425:1441	When transferring film manufacturing from lab-scale to continuous mode, film compositions, processing conditions and suitable characterization methods have to be carefully selected and adopted.
29146537	6	43	theme	dynamic	935:941	arg1	viscosity					943:951	at least a dynamic viscosity	924:951	at least a dynamic viscosity of 1 Pa*s (wet film thickness of 500 μm, velocity of 15.9 cm/min)	924:1017	Coating solutions for continuous manufacturing should provide at least a dynamic viscosity of 1 Pa*s (wet film thickness of 500 μm, velocity of 15.9 cm/min).
29146537	2	44	theme	film	250:253	arg1	formulations					255:266	film formulations	250:266	film formulations	250:266	Due to various production settings, different requirements on film formulations are required for non- continuous and continuous manufacturing.
29146537	3	45	theme	film	430:433	arg1	compositions					435:446	film compositions	430:446	film compositions	430:446	In this study, a continuous coating machine was qualified in regards of the process conditions for film compositions and their effects on the formed films.
29146537	4	46	theme	various	552:558	arg1	formulations					565:576	various film formulations	552:576	various film formulations of hydrochlorothiazide and hydroxypropylcellulose (HPC) or hydroxypropylmethycellulose (HPMC) as film formers	552:686	To investigate differences between both manufacturing processes, various film formulations of hydrochlorothiazide and hydroxypropylcellulose (HPC) or hydroxypropylmethycellulose (HPMC) as film formers were produced and the resulting films were characterized.
29146537	7	47	theme	continuous	1100:1109	arg1	mode					1122:1125	continuous production mode	1100:1125	continuous production mode	1100:1125	HPC films contain higher residuals of ethanol or acetone in bench-scale than in continuous production mode.
29146537	8	48	theme	films	1184:1188	arg1	content					1169:1175	lower drug content	1158:1175	lower drug content of the films	1158:1188	Continuous production lead to lower drug content of the films.
29146537	7	49	theme	ethanol	1058:1064	arg1	residuals					1045:1053	higher residuals	1038:1053	higher residuals of ethanol or acetone in bench-scale	1038:1090	HPC films contain higher residuals of ethanol or acetone in bench-scale than in continuous production mode.
29146537	11	50	theme	characterization	1479:1494	arg1	methods					1496:1502	suitable characterization methods	1470:1502	suitable characterization methods	1470:1502	When transferring film manufacturing from lab-scale to continuous mode, film compositions, processing conditions and suitable characterization methods have to be carefully selected and adopted.
29146537	3	51	theme	process	407:413	arg1	conditions					415:424	the process conditions	403:424	the process conditions	403:424	In this study, a continuous coating machine was qualified in regards of the process conditions for film compositions and their effects on the formed films.
29146537	8	52	theme	lower	1158:1162	arg1	content					1169:1175	lower drug content	1158:1175	lower drug content of the films	1158:1188	Continuous production lead to lower drug content of the films.
29146537	3	53	theme	continuous	348:357	arg1	machine					367:373	a continuous coating machine	346:373	a continuous coating machine	346:373	In this study, a continuous coating machine was qualified in regards of the process conditions for film compositions and their effects on the formed films.
29146537	3	54	theme	conditions	415:424	arg1	regards					392:398	regards	392:398	regards of the process conditions	392:424	In this study, a continuous coating machine was qualified in regards of the process conditions for film compositions and their effects on the formed films.
29146537	6	55	theme	500 μm	986:991	arg1	thickness					973:981	wet film thickness	964:981	wet film thickness of 500 μm, velocity of 15.9 cm/min	964:1016	Coating solutions for continuous manufacturing should provide at least a dynamic viscosity of 1 Pa*s (wet film thickness of 500 μm, velocity of 15.9 cm/min).
29146537	7	56	theme	production	1111:1120	arg1	mode					1122:1125	continuous production mode	1100:1125	continuous production mode	1100:1125	HPC films contain higher residuals of ethanol or acetone in bench-scale than in continuous production mode.
29146537	2	57	theme	different	224:232	arg1	requirements					234:245	different requirements	224:245	different requirements on film formulations	224:266	Due to various production settings, different requirements on film formulations are required for non- continuous and continuous manufacturing.
29146537	1	58	theme	new	127:129	arg1	films					98:102	Orodispersible films	83:102	Orodispersible films	83:102	Orodispersible films have been described as new beneficial dosage forms for special patient populations.
29146537	1	58	theme	new	127:129	arg1	forms					149:153	new beneficial dosage forms	127:153	new beneficial dosage forms for special patient populations	127:185	Orodispersible films have been described as new beneficial dosage forms for special patient populations.
29146537	4	59	theme	film	560:563	arg1	formulations					565:576	various film formulations	552:576	various film formulations of hydrochlorothiazide and hydroxypropylcellulose (HPC) or hydroxypropylmethycellulose (HPMC) as film formers	552:686	To investigate differences between both manufacturing processes, various film formulations of hydrochlorothiazide and hydroxypropylcellulose (HPC) or hydroxypropylmethycellulose (HPMC) as film formers were produced and the resulting films were characterized.
29146537	7	60	theme	acetone	1069:1075	arg1	residuals					1045:1053	higher residuals	1038:1053	higher residuals of ethanol or acetone in bench-scale	1038:1090	HPC films contain higher residuals of ethanol or acetone in bench-scale than in continuous production mode.
29146537	8	61	theme	drug	1164:1167	arg1	content					1169:1175	lower drug content	1158:1175	lower drug content of the films	1158:1188	Continuous production lead to lower drug content of the films.
29146537	1	62	theme	beneficial	131:140	arg1	films					98:102	Orodispersible films	83:102	Orodispersible films	83:102	Orodispersible films have been described as new beneficial dosage forms for special patient populations.
29146537	1	62	theme	beneficial	131:140	arg1	forms					149:153	new beneficial dosage forms	127:153	new beneficial dosage forms for special patient populations	127:185	Orodispersible films have been described as new beneficial dosage forms for special patient populations.
29146537	1	63	theme	Orodispersible	83:96	arg1	forms					149:153	new beneficial dosage forms	127:153	new beneficial dosage forms for special patient populations	127:185	Orodispersible films have been described as new beneficial dosage forms for special patient populations.
29146537	1	63	theme	Orodispersible	83:96	arg1	films					98:102	Orodispersible films	83:102	Orodispersible films	83:102	Orodispersible films have been described as new beneficial dosage forms for special patient populations.
29146537	2	64	theme	production	203:212	arg1	settings					214:221	various production settings	195:221	various production settings	195:221	Due to various production settings, different requirements on film formulations are required for non- continuous and continuous manufacturing.
29146537	3	65	from	compositions	435:446	arg1	films					480:484	the formed films	469:484	the formed films	469:484	In this study, a continuous coating machine was qualified in regards of the process conditions for film compositions and their effects on the formed films.
29146537	1	66	theme	dosage	142:147	arg1	films					98:102	Orodispersible films	83:102	Orodispersible films	83:102	Orodispersible films have been described as new beneficial dosage forms for special patient populations.
29146537	1	66	theme	dosage	142:147	arg1	forms					149:153	new beneficial dosage forms	127:153	new beneficial dosage forms for special patient populations	127:185	Orodispersible films have been described as new beneficial dosage forms for special patient populations.
29146537	10	67	theme	significant	1278:1288	arg1	effects					1290:1296	observed significant effects	1269:1296	observed significant effects of the production process on the film characteristics	1269:1350	There are observed significant effects of the production process on the film characteristics.
29146537	4	68	theme	hydroxypropylmethycellulose	637:663	arg1	formulations					565:576	various film formulations	552:576	various film formulations of hydrochlorothiazide and hydroxypropylcellulose (HPC) or hydroxypropylmethycellulose (HPMC) as film formers	552:686	To investigate differences between both manufacturing processes, various film formulations of hydrochlorothiazide and hydroxypropylcellulose (HPC) or hydroxypropylmethycellulose (HPMC) as film formers were produced and the resulting films were characterized.
29146537	2	69	theme	various	195:201	arg1	settings					214:221	various production settings	195:221	various production settings	195:221	Due to various production settings, different requirements on film formulations are required for non- continuous and continuous manufacturing.
29146537	4	70	theme	film	675:678	arg1	formers					680:686	film formers	675:686	film formers	675:686	To investigate differences between both manufacturing processes, various film formulations of hydrochlorothiazide and hydroxypropylcellulose (HPC) or hydroxypropylmethycellulose (HPMC) as film formers were produced and the resulting films were characterized.
29146537	10	71	theme	observed	1269:1276	arg1	effects					1290:1296	observed significant effects	1269:1296	observed significant effects of the production process on the film characteristics	1269:1350	There are observed significant effects of the production process on the film characteristics.
29146537	6	72	dep	s	961:961	arg1	thickness					973:981	wet film thickness	964:981	wet film thickness of 500 μm, velocity of 15.9 cm/min	964:1016	Coating solutions for continuous manufacturing should provide at least a dynamic viscosity of 1 Pa*s (wet film thickness of 500 μm, velocity of 15.9 cm/min).
29146537	0	73	theme	continuous	57:66	arg1	manufacturing					68:80	continuous manufacturing	57:80	continuous manufacturing	57:80	Orodispersible films: Product transfer from lab-scale to continuous manufacturing.
29146537	7	74	theme	HPC	1020:1022	arg1	films					1024:1028	HPC films	1020:1028	HPC films	1020:1028	HPC films contain higher residuals of ethanol or acetone in bench-scale than in continuous production mode.
29146537	11	75	theme	processing	1444:1453	arg1	conditions					1455:1464	processing conditions	1444:1464	processing conditions	1444:1464	When transferring film manufacturing from lab-scale to continuous mode, film compositions, processing conditions and suitable characterization methods have to be carefully selected and adopted.
25188309	2	0	theme	composites	412:421	arg1	crystallinity					391:403	crystallinity	391:403	crystallinity	391:403	It was shown for the first time that simple control of the onset of biomineralization could enable fine tuning of the composition and crystallinity of the composites to determine their photocatalytic performance.
25188309	2	0	theme	composites	412:421	arg1	composition					375:385	composition	375:385	composition	375:385	It was shown for the first time that simple control of the onset of biomineralization could enable fine tuning of the composition and crystallinity of the composites to determine their photocatalytic performance.
25188309	2	1	theme	crystallinity	391:403	arg1	tuning					361:366	fine tuning	356:366	fine tuning of the composition and crystallinity of the composites	356:421	It was shown for the first time that simple control of the onset of biomineralization could enable fine tuning of the composition and crystallinity of the composites to determine their photocatalytic performance.
25188309	2	2	theme	fine	356:359	arg1	tuning					361:366	fine tuning	356:366	fine tuning of the composition and crystallinity of the composites	356:421	It was shown for the first time that simple control of the onset of biomineralization could enable fine tuning of the composition and crystallinity of the composites to determine their photocatalytic performance.
25188309	2	3	theme	composition	375:385	arg1	tuning					361:366	fine tuning	356:366	fine tuning of the composition and crystallinity of the composites	356:421	It was shown for the first time that simple control of the onset of biomineralization could enable fine tuning of the composition and crystallinity of the composites to determine their photocatalytic performance.
25188309	2	4	theme	biomineralization	325:341	arg1	onset					316:320	the onset	312:320	the onset of biomineralization	312:341	It was shown for the first time that simple control of the onset of biomineralization could enable fine tuning of the composition and crystallinity of the composites to determine their photocatalytic performance.
25188309	1	5	with	composites	143:152	arg1	activity					191:198	greatly enhanced photocatalytic activity	159:198	greatly enhanced photocatalytic activity	159:198	Titanium-tungsten oxide composites with greatly enhanced photocatalytic activity were synthesized by lysozyme-mediated biomineralization.
25188309	1	6	theme	Titanium-tungsten	119:135	arg1	composites					143:152	Titanium-tungsten oxide composites	119:152	Titanium-tungsten oxide composites with greatly enhanced photocatalytic activity	119:198	Titanium-tungsten oxide composites with greatly enhanced photocatalytic activity were synthesized by lysozyme-mediated biomineralization.
25188309	0	7	theme	Lysozyme-mediated	0:16	arg1	biomineralization					18:34	Lysozyme-mediated biomineralization	0:34	Lysozyme-mediated biomineralization of titanium-tungsten oxide hybrid	0:68	Lysozyme-mediated biomineralization of titanium-tungsten oxide hybrid nanoparticles with high photocatalytic activity.
25188309	1	8	theme	oxide	137:141	arg1	composites					143:152	Titanium-tungsten oxide composites	119:152	Titanium-tungsten oxide composites with greatly enhanced photocatalytic activity	119:198	Titanium-tungsten oxide composites with greatly enhanced photocatalytic activity were synthesized by lysozyme-mediated biomineralization.
25188309	2	9	theme	photocatalytic	442:455	arg1	performance					457:467	their photocatalytic performance	436:467	their photocatalytic performance	436:467	It was shown for the first time that simple control of the onset of biomineralization could enable fine tuning of the composition and crystallinity of the composites to determine their photocatalytic performance.
25188309	0	10	theme	titanium-tungsten	39:55	arg1	hybrid					63:68	titanium-tungsten oxide hybrid	39:68	titanium-tungsten oxide hybrid	39:68	Lysozyme-mediated biomineralization of titanium-tungsten oxide hybrid nanoparticles with high photocatalytic activity.
25188309	1	11	theme	lysozyme-mediated	220:236	arg1	biomineralization					238:254	lysozyme-mediated biomineralization	220:254	lysozyme-mediated biomineralization	220:254	Titanium-tungsten oxide composites with greatly enhanced photocatalytic activity were synthesized by lysozyme-mediated biomineralization.
25188309	0	12	theme	hybrid	63:68	arg1	biomineralization					18:34	Lysozyme-mediated biomineralization	0:34	Lysozyme-mediated biomineralization of titanium-tungsten oxide hybrid	0:68	Lysozyme-mediated biomineralization of titanium-tungsten oxide hybrid nanoparticles with high photocatalytic activity.
25188309	0	13	theme	oxide	57:61	arg1	hybrid					63:68	titanium-tungsten oxide hybrid	39:68	titanium-tungsten oxide hybrid	39:68	Lysozyme-mediated biomineralization of titanium-tungsten oxide hybrid nanoparticles with high photocatalytic activity.
25188309	2	14	theme	simple	294:299	arg1	control					301:307	simple control	294:307	simple control of the onset of biomineralization	294:341	It was shown for the first time that simple control of the onset of biomineralization could enable fine tuning of the composition and crystallinity of the composites to determine their photocatalytic performance.
25188309	2	15	theme	first	278:282	arg1	time					284:287	the first time	274:287	the first time	274:287	It was shown for the first time that simple control of the onset of biomineralization could enable fine tuning of the composition and crystallinity of the composites to determine their photocatalytic performance.
25188309	1	16	theme	enhanced	167:174	arg1	activity					191:198	greatly enhanced photocatalytic activity	159:198	greatly enhanced photocatalytic activity	159:198	Titanium-tungsten oxide composites with greatly enhanced photocatalytic activity were synthesized by lysozyme-mediated biomineralization.
25188309	1	17	theme	photocatalytic	176:189	arg1	activity					191:198	greatly enhanced photocatalytic activity	159:198	greatly enhanced photocatalytic activity	159:198	Titanium-tungsten oxide composites with greatly enhanced photocatalytic activity were synthesized by lysozyme-mediated biomineralization.
25188309	2	18	theme	onset	316:320	arg1	control					301:307	simple control	294:307	simple control of the onset of biomineralization	294:341	It was shown for the first time that simple control of the onset of biomineralization could enable fine tuning of the composition and crystallinity of the composites to determine their photocatalytic performance.
25188309	2	19	dep	composition	375:385	arg1	the					371:373	the	371:373	the	371:373	It was shown for the first time that simple control of the onset of biomineralization could enable fine tuning of the composition and crystallinity of the composites to determine their photocatalytic performance.
25188309	0	20	theme	photocatalytic	94:107	arg1	activity					109:116	high photocatalytic activity	89:116	high photocatalytic activity	89:116	Lysozyme-mediated biomineralization of titanium-tungsten oxide hybrid nanoparticles with high photocatalytic activity.
25188309	0	21	theme	high	89:92	arg1	activity					109:116	high photocatalytic activity	89:116	high photocatalytic activity	89:116	Lysozyme-mediated biomineralization of titanium-tungsten oxide hybrid nanoparticles with high photocatalytic activity.
26724686	4	0	theme	polysaccharide	463:476	arg1	weight					444:449	The molecular weight	430:449	The molecular weight of sulfated polysaccharide determined through PAGE	430:500	The molecular weight of sulfated polysaccharide determined through PAGE was found to be as 66 kDa.
26724686	0	1	from	characterization	11:26	arg1	gladius					90:96	the gladius	86:96	the gladius of Sepioteuthis lessoniana (Lesson, 1831)	86:138	Structural characterization and biomedical properties of sulfated polysaccharide from the gladius of Sepioteuthis lessoniana (Lesson, 1831).
26724686	2	2	theme	ash	328:330	arg1	%					305:305	0.8%	302:305	0.8% of protein	302:316	The sulfated polysaccharide contained 61.3% of carbohydrate, 0.8% of protein, 28.2% of ash and 1.33% of moisture respectively.
26724686	2	2	theme	ash	328:330	arg1	ash					328:330	ash	328:330	ash	328:330	The sulfated polysaccharide contained 61.3% of carbohydrate, 0.8% of protein, 28.2% of ash and 1.33% of moisture respectively.
26724686	2	2	theme	ash	328:330	arg1	protein					310:316	protein	310:316	protein	310:316	The sulfated polysaccharide contained 61.3% of carbohydrate, 0.8% of protein, 28.2% of ash and 1.33% of moisture respectively.
26724686	2	2	theme	ash	328:330	arg1	%					283:283	61.3%	279:283	61.3% of carbohydrate	279:299	The sulfated polysaccharide contained 61.3% of carbohydrate, 0.8% of protein, 28.2% of ash and 1.33% of moisture respectively.
26724686	2	2	theme	ash	328:330	arg1	%					340:340	1.33%	336:340	1.33% of moisture	336:352	The sulfated polysaccharide contained 61.3% of carbohydrate, 0.8% of protein, 28.2% of ash and 1.33% of moisture respectively.
26724686	2	2	theme	ash	328:330	arg1	carbohydrate					288:299	carbohydrate	288:299	carbohydrate	288:299	The sulfated polysaccharide contained 61.3% of carbohydrate, 0.8% of protein, 28.2% of ash and 1.33% of moisture respectively.
26724686	2	2	theme	ash	328:330	arg1	%					323:323	28.2%	319:323	28.2% of ash	319:330	The sulfated polysaccharide contained 61.3% of carbohydrate, 0.8% of protein, 28.2% of ash and 1.33% of moisture respectively.
26724686	2	2	theme	ash	328:330	arg1	moisture					345:352	moisture	345:352	moisture	345:352	The sulfated polysaccharide contained 61.3% of carbohydrate, 0.8% of protein, 28.2% of ash and 1.33% of moisture respectively.
26724686	9	3	theme	cell	1269:1272	arg1	line					1274:1277	HeLa cell line	1264:1277	HeLa cell line	1264:1277	The anticancer activity was tested against HeLa cell line by MTT assay.
26724686	0	4	theme	lessoniana	114:123	arg1	gladius					90:96	the gladius	86:96	the gladius of Sepioteuthis lessoniana (Lesson, 1831)	86:138	Structural characterization and biomedical properties of sulfated polysaccharide from the gladius of Sepioteuthis lessoniana (Lesson, 1831).
26724686	11	5	theme	anticancer	1617:1626	arg1	acitivity					1628:1636	good antibacterial and anticancer acitivity	1594:1636	good antibacterial and anticancer acitivity	1594:1636	In conclusion, the sulfated polysaccharide is an alternate, non-toxic and cheap source of substance that showed good antibacterial and anticancer acitivity.
26724686	10	6	theme	%	1398:1398	arg1	activity					1381:1388	the maximum anticancer activity	1358:1388	the maximum anticancer activity of 62.89%	1358:1398	The Cytotoxic Concentration (CC50) was observed as 700 μg/ml and the maximum anticancer activity of 62.89% was recorded at 200 μg/ml; whereas, the lowest of 9.87% was observed at 25 μg/ml.
26724686	11	7	theme	sulfated	1501:1508	arg1	polysaccharide					1510:1523	the sulfated polysaccharide	1497:1523	the sulfated polysaccharide	1497:1523	In conclusion, the sulfated polysaccharide is an alternate, non-toxic and cheap source of substance that showed good antibacterial and anticancer acitivity.
26724686	11	7	theme	sulfated	1501:1508	arg1	source					1562:1567	an alternate, non-toxic and cheap source	1528:1567	an alternate, non-toxic and cheap source of substance that showed good antibacterial and anticancer acitivity	1528:1636	In conclusion, the sulfated polysaccharide is an alternate, non-toxic and cheap source of substance that showed good antibacterial and anticancer acitivity.
26724686	6	8	theme	FT-IR	717:721	arg1	spectroscopy					731:742	FT-IR and NMR spectroscopy	717:742	spectroscopy	731:742	The structural features of sulfated polysaccharide were analyzed by FT-IR and NMR spectroscopy.
26724686	2	9	contain	contained	269:277	arg2	%					283:283	61.3%	279:283	61.3% of carbohydrate	279:299	The sulfated polysaccharide contained 61.3% of carbohydrate, 0.8% of protein, 28.2% of ash and 1.33% of moisture respectively.
26724686	2	9	contain	contained	269:277	arg2	carbohydrate					288:299	carbohydrate	288:299	carbohydrate	288:299	The sulfated polysaccharide contained 61.3% of carbohydrate, 0.8% of protein, 28.2% of ash and 1.33% of moisture respectively.
26724686	2	9	contain	contained	269:277	arg2	%					305:305	0.8%	302:305	0.8% of protein	302:316	The sulfated polysaccharide contained 61.3% of carbohydrate, 0.8% of protein, 28.2% of ash and 1.33% of moisture respectively.
26724686	2	9	contain	contained	269:277	arg2	moisture					345:352	moisture	345:352	moisture	345:352	The sulfated polysaccharide contained 61.3% of carbohydrate, 0.8% of protein, 28.2% of ash and 1.33% of moisture respectively.
26724686	2	9	contain	contained	269:277	arg2	%					323:323	28.2%	319:323	28.2% of ash	319:330	The sulfated polysaccharide contained 61.3% of carbohydrate, 0.8% of protein, 28.2% of ash and 1.33% of moisture respectively.
26724686	2	9	contain	contained	269:277	arg2	protein					310:316	protein	310:316	protein	310:316	The sulfated polysaccharide contained 61.3% of carbohydrate, 0.8% of protein, 28.2% of ash and 1.33% of moisture respectively.
26724686	2	9	contain	contained	269:277	arg2	ash					328:330	ash	328:330	ash	328:330	The sulfated polysaccharide contained 61.3% of carbohydrate, 0.8% of protein, 28.2% of ash and 1.33% of moisture respectively.
26724686	2	9	contain	contained	269:277	arg2	%					340:340	1.33%	336:340	1.33% of moisture	336:352	The sulfated polysaccharide contained 61.3% of carbohydrate, 0.8% of protein, 28.2% of ash and 1.33% of moisture respectively.
26724686	2	9	contain	contained	269:277	arg1	polysaccharide					254:267	The sulfated polysaccharide	241:267	The sulfated polysaccharide	241:267	The sulfated polysaccharide contained 61.3% of carbohydrate, 0.8% of protein, 28.2% of ash and 1.33% of moisture respectively.
26724686	4	10	theme	sulfated	454:461	arg1	polysaccharide					463:476	sulfated polysaccharide	454:476	sulfated polysaccharide	454:476	The molecular weight of sulfated polysaccharide determined through PAGE was found to be as 66 kDa.
26724686	9	11	theme	anticancer	1225:1234	arg1	activity					1236:1243	The anticancer activity	1221:1243	The anticancer activity	1221:1243	The anticancer activity was tested against HeLa cell line by MTT assay.
26724686	2	12	theme	carbohydrate	288:299	arg1	%					305:305	0.8%	302:305	0.8% of protein	302:316	The sulfated polysaccharide contained 61.3% of carbohydrate, 0.8% of protein, 28.2% of ash and 1.33% of moisture respectively.
26724686	2	12	theme	carbohydrate	288:299	arg1	ash					328:330	ash	328:330	ash	328:330	The sulfated polysaccharide contained 61.3% of carbohydrate, 0.8% of protein, 28.2% of ash and 1.33% of moisture respectively.
26724686	2	12	theme	carbohydrate	288:299	arg1	protein					310:316	protein	310:316	protein	310:316	The sulfated polysaccharide contained 61.3% of carbohydrate, 0.8% of protein, 28.2% of ash and 1.33% of moisture respectively.
26724686	2	12	theme	carbohydrate	288:299	arg1	%					283:283	61.3%	279:283	61.3% of carbohydrate	279:299	The sulfated polysaccharide contained 61.3% of carbohydrate, 0.8% of protein, 28.2% of ash and 1.33% of moisture respectively.
26724686	2	12	theme	carbohydrate	288:299	arg1	%					340:340	1.33%	336:340	1.33% of moisture	336:352	The sulfated polysaccharide contained 61.3% of carbohydrate, 0.8% of protein, 28.2% of ash and 1.33% of moisture respectively.
26724686	2	12	theme	carbohydrate	288:299	arg1	carbohydrate					288:299	carbohydrate	288:299	carbohydrate	288:299	The sulfated polysaccharide contained 61.3% of carbohydrate, 0.8% of protein, 28.2% of ash and 1.33% of moisture respectively.
26724686	2	12	theme	carbohydrate	288:299	arg1	%					323:323	28.2%	319:323	28.2% of ash	319:330	The sulfated polysaccharide contained 61.3% of carbohydrate, 0.8% of protein, 28.2% of ash and 1.33% of moisture respectively.
26724686	2	12	theme	carbohydrate	288:299	arg1	moisture					345:352	moisture	345:352	moisture	345:352	The sulfated polysaccharide contained 61.3% of carbohydrate, 0.8% of protein, 28.2% of ash and 1.33% of moisture respectively.
26724686	7	13	dep	Vibrio	940:945	arg1	cholerae					947:954	Vibrio cholerae	940:954	Vibrio cholerae	940:954	Further the sulfated polysaccharide was evaluated for its antibacterial activity against selected human clinical pathogens, namely Staphylococcus aureus, Klebsiella pneumoniae, Salmonella typhi, Vibrio cholerae, Klebsiella oxytoca, Escherichia coli, Salmonella paratyphi, Proteus mirabilis, Vibrio parahaemolyticus and Streptococcus pyogenes using agar well diffusion method.
26724686	0	14	dep	lessoniana	114:123	arg1	1831					134:137	1831	134:137	1831	134:137	Structural characterization and biomedical properties of sulfated polysaccharide from the gladius of Sepioteuthis lessoniana (Lesson, 1831).
26724686	0	14	dep	lessoniana	114:123	arg1	Lesson					126:131	Lesson	126:131	Lesson	126:131	Structural characterization and biomedical properties of sulfated polysaccharide from the gladius of Sepioteuthis lessoniana (Lesson, 1831).
26724686	1	15	theme	internal	188:195	arg1	gladius					204:210	gladius	204:210	gladius	204:210	Sulfated polysaccharide was extracted from the internal shell (gladius) of Sepioteuthis lessoniana.
26724686	1	15	theme	internal	188:195	arg1	shell					197:201	the internal shell	184:201	the internal shell (gladius) of Sepioteuthis lessoniana	184:238	Sulfated polysaccharide was extracted from the internal shell (gladius) of Sepioteuthis lessoniana.
26724686	3	16	theme	CHNS/O	413:418	arg1	analyzer					420:427	CHNS/O analyzer	413:427	CHNS/O analyzer	413:427	The elemental composition was analyzed using CHNS/O analyzer.
26724686	3	17	theme	elemental	372:380	arg1	composition					382:392	The elemental composition	368:392	The elemental composition	368:392	The elemental composition was analyzed using CHNS/O analyzer.
26724686	11	18	theme	non-toxic	1542:1550	arg1	source					1562:1567	an alternate, non-toxic and cheap source	1528:1567	an alternate, non-toxic and cheap source of substance that showed good antibacterial and anticancer acitivity	1528:1636	In conclusion, the sulfated polysaccharide is an alternate, non-toxic and cheap source of substance that showed good antibacterial and anticancer acitivity.
26724686	11	18	theme	non-toxic	1542:1550	arg1	polysaccharide					1510:1523	the sulfated polysaccharide	1497:1523	the sulfated polysaccharide	1497:1523	In conclusion, the sulfated polysaccharide is an alternate, non-toxic and cheap source of substance that showed good antibacterial and anticancer acitivity.
26724686	7	19	theme	sulfated	757:764	arg1	polysaccharide					766:779	Further the sulfated polysaccharide	745:779	Further the sulfated polysaccharide	745:779	Further the sulfated polysaccharide was evaluated for its antibacterial activity against selected human clinical pathogens, namely Staphylococcus aureus, Klebsiella pneumoniae, Salmonella typhi, Vibrio cholerae, Klebsiella oxytoca, Escherichia coli, Salmonella paratyphi, Proteus mirabilis, Vibrio parahaemolyticus and Streptococcus pyogenes using agar well diffusion method.
26724686	10	20	theme	anticancer	1370:1379	arg1	activity					1381:1388	the maximum anticancer activity	1358:1388	the maximum anticancer activity of 62.89%	1358:1398	The Cytotoxic Concentration (CC50) was observed as 700 μg/ml and the maximum anticancer activity of 62.89% was recorded at 200 μg/ml; whereas, the lowest of 9.87% was observed at 25 μg/ml.
26724686	10	21	located	observed	1460:1467	arg1	μg/ml					1475:1479	25 μg/ml	1472:1479	25 μg/ml	1472:1479	The Cytotoxic Concentration (CC50) was observed as 700 μg/ml and the maximum anticancer activity of 62.89% was recorded at 200 μg/ml; whereas, the lowest of 9.87% was observed at 25 μg/ml.
26724686	10	21	located	observed	1460:1467	arg2	lowest					1440:1445	lowest	1440:1445	lowest	1440:1445	The Cytotoxic Concentration (CC50) was observed as 700 μg/ml and the maximum anticancer activity of 62.89% was recorded at 200 μg/ml; whereas, the lowest of 9.87% was observed at 25 μg/ml.
26724686	0	22	from	properties	43:52	arg1	gladius					90:96	the gladius	86:96	the gladius of Sepioteuthis lessoniana (Lesson, 1831)	86:138	Structural characterization and biomedical properties of sulfated polysaccharide from the gladius of Sepioteuthis lessoniana (Lesson, 1831).
26724686	7	23	dep	Klebsiella	899:908	arg1	pneumoniae					910:919	Klebsiella pneumoniae	899:919	Klebsiella pneumoniae	899:919	Further the sulfated polysaccharide was evaluated for its antibacterial activity against selected human clinical pathogens, namely Staphylococcus aureus, Klebsiella pneumoniae, Salmonella typhi, Vibrio cholerae, Klebsiella oxytoca, Escherichia coli, Salmonella paratyphi, Proteus mirabilis, Vibrio parahaemolyticus and Streptococcus pyogenes using agar well diffusion method.
26724686	2	24	theme	protein	310:316	arg1	%					305:305	0.8%	302:305	0.8% of protein	302:316	The sulfated polysaccharide contained 61.3% of carbohydrate, 0.8% of protein, 28.2% of ash and 1.33% of moisture respectively.
26724686	2	24	theme	protein	310:316	arg1	ash					328:330	ash	328:330	ash	328:330	The sulfated polysaccharide contained 61.3% of carbohydrate, 0.8% of protein, 28.2% of ash and 1.33% of moisture respectively.
26724686	2	24	theme	protein	310:316	arg1	protein					310:316	protein	310:316	protein	310:316	The sulfated polysaccharide contained 61.3% of carbohydrate, 0.8% of protein, 28.2% of ash and 1.33% of moisture respectively.
26724686	2	24	theme	protein	310:316	arg1	%					283:283	61.3%	279:283	61.3% of carbohydrate	279:299	The sulfated polysaccharide contained 61.3% of carbohydrate, 0.8% of protein, 28.2% of ash and 1.33% of moisture respectively.
26724686	2	24	theme	protein	310:316	arg1	%					340:340	1.33%	336:340	1.33% of moisture	336:352	The sulfated polysaccharide contained 61.3% of carbohydrate, 0.8% of protein, 28.2% of ash and 1.33% of moisture respectively.
26724686	2	24	theme	protein	310:316	arg1	carbohydrate					288:299	carbohydrate	288:299	carbohydrate	288:299	The sulfated polysaccharide contained 61.3% of carbohydrate, 0.8% of protein, 28.2% of ash and 1.33% of moisture respectively.
26724686	2	24	theme	protein	310:316	arg1	%					323:323	28.2%	319:323	28.2% of ash	319:330	The sulfated polysaccharide contained 61.3% of carbohydrate, 0.8% of protein, 28.2% of ash and 1.33% of moisture respectively.
26724686	2	24	theme	protein	310:316	arg1	moisture					345:352	moisture	345:352	moisture	345:352	The sulfated polysaccharide contained 61.3% of carbohydrate, 0.8% of protein, 28.2% of ash and 1.33% of moisture respectively.
26724686	6	25	theme	polysaccharide	685:698	arg1	features					664:671	The structural features	649:671	The structural features of sulfated polysaccharide	649:698	The structural features of sulfated polysaccharide were analyzed by FT-IR and NMR spectroscopy.
26724686	2	26	theme	moisture	345:352	arg1	%					305:305	0.8%	302:305	0.8% of protein	302:316	The sulfated polysaccharide contained 61.3% of carbohydrate, 0.8% of protein, 28.2% of ash and 1.33% of moisture respectively.
26724686	2	26	theme	moisture	345:352	arg1	ash					328:330	ash	328:330	ash	328:330	The sulfated polysaccharide contained 61.3% of carbohydrate, 0.8% of protein, 28.2% of ash and 1.33% of moisture respectively.
26724686	2	26	theme	moisture	345:352	arg1	protein					310:316	protein	310:316	protein	310:316	The sulfated polysaccharide contained 61.3% of carbohydrate, 0.8% of protein, 28.2% of ash and 1.33% of moisture respectively.
26724686	2	26	theme	moisture	345:352	arg1	%					283:283	61.3%	279:283	61.3% of carbohydrate	279:299	The sulfated polysaccharide contained 61.3% of carbohydrate, 0.8% of protein, 28.2% of ash and 1.33% of moisture respectively.
26724686	2	26	theme	moisture	345:352	arg1	%					340:340	1.33%	336:340	1.33% of moisture	336:352	The sulfated polysaccharide contained 61.3% of carbohydrate, 0.8% of protein, 28.2% of ash and 1.33% of moisture respectively.
26724686	2	26	theme	moisture	345:352	arg1	carbohydrate					288:299	carbohydrate	288:299	carbohydrate	288:299	The sulfated polysaccharide contained 61.3% of carbohydrate, 0.8% of protein, 28.2% of ash and 1.33% of moisture respectively.
26724686	2	26	theme	moisture	345:352	arg1	%					323:323	28.2%	319:323	28.2% of ash	319:330	The sulfated polysaccharide contained 61.3% of carbohydrate, 0.8% of protein, 28.2% of ash and 1.33% of moisture respectively.
26724686	2	26	theme	moisture	345:352	arg1	moisture					345:352	moisture	345:352	moisture	345:352	The sulfated polysaccharide contained 61.3% of carbohydrate, 0.8% of protein, 28.2% of ash and 1.33% of moisture respectively.
26724686	0	27	theme	Structural	0:9	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and biomedical properties of sulfated polysaccharide from the gladius of Sepioteuthis lessoniana (Lesson, 1831).
26724686	5	28	theme	sulfated	568:575	arg1	polysaccharide					577:590	sulfated polysaccharide	568:590	sulfated polysaccharide	568:590	Monosaccharides analysis revealed that sulfated polysaccharide was composed of rhamnose, galactose, xylose and glucose.
26724686	6	29	theme	sulfated	676:683	arg1	polysaccharide					685:698	sulfated polysaccharide	676:698	sulfated polysaccharide	676:698	The structural features of sulfated polysaccharide were analyzed by FT-IR and NMR spectroscopy.
26724686	0	30	theme	biomedical	32:41	arg1	properties					43:52	biomedical properties	32:52	biomedical properties	32:52	Structural characterization and biomedical properties of sulfated polysaccharide from the gladius of Sepioteuthis lessoniana (Lesson, 1831).
26724686	11	31	theme	alternate	1531:1539	arg1	source					1562:1567	an alternate, non-toxic and cheap source	1528:1567	an alternate, non-toxic and cheap source of substance that showed good antibacterial and anticancer acitivity	1528:1636	In conclusion, the sulfated polysaccharide is an alternate, non-toxic and cheap source of substance that showed good antibacterial and anticancer acitivity.
26724686	11	31	theme	alternate	1531:1539	arg1	polysaccharide					1510:1523	the sulfated polysaccharide	1497:1523	the sulfated polysaccharide	1497:1523	In conclusion, the sulfated polysaccharide is an alternate, non-toxic and cheap source of substance that showed good antibacterial and anticancer acitivity.
26724686	7	32	theme	diffusion	1103:1111	arg1	method					1113:1118	diffusion method	1103:1118	diffusion method	1103:1118	Further the sulfated polysaccharide was evaluated for its antibacterial activity against selected human clinical pathogens, namely Staphylococcus aureus, Klebsiella pneumoniae, Salmonella typhi, Vibrio cholerae, Klebsiella oxytoca, Escherichia coli, Salmonella paratyphi, Proteus mirabilis, Vibrio parahaemolyticus and Streptococcus pyogenes using agar well diffusion method.
26724686	9	33	theme	HeLa	1264:1267	arg1	line					1274:1277	HeLa cell line	1264:1277	HeLa cell line	1264:1277	The anticancer activity was tested against HeLa cell line by MTT assay.
26724686	11	34	theme	antibacterial	1599:1611	arg1	acitivity					1628:1636	good antibacterial and anticancer acitivity	1594:1636	good antibacterial and anticancer acitivity	1594:1636	In conclusion, the sulfated polysaccharide is an alternate, non-toxic and cheap source of substance that showed good antibacterial and anticancer acitivity.
26724686	11	35	theme	cheap	1556:1560	arg1	source					1562:1567	an alternate, non-toxic and cheap source	1528:1567	an alternate, non-toxic and cheap source of substance that showed good antibacterial and anticancer acitivity	1528:1636	In conclusion, the sulfated polysaccharide is an alternate, non-toxic and cheap source of substance that showed good antibacterial and anticancer acitivity.
26724686	11	35	theme	cheap	1556:1560	arg1	polysaccharide					1510:1523	the sulfated polysaccharide	1497:1523	the sulfated polysaccharide	1497:1523	In conclusion, the sulfated polysaccharide is an alternate, non-toxic and cheap source of substance that showed good antibacterial and anticancer acitivity.
26724686	9	36	theme	MTT	1282:1284	arg1	assay					1286:1290	MTT assay	1282:1290	MTT assay	1282:1290	The anticancer activity was tested against HeLa cell line by MTT assay.
26724686	7	37	theme	human	843:847	arg1	typhi					933:937	Salmonella typhi	922:937	Salmonella typhi	922:937	Further the sulfated polysaccharide was evaluated for its antibacterial activity against selected human clinical pathogens, namely Staphylococcus aureus, Klebsiella pneumoniae, Salmonella typhi, Vibrio cholerae, Klebsiella oxytoca, Escherichia coli, Salmonella paratyphi, Proteus mirabilis, Vibrio parahaemolyticus and Streptococcus pyogenes using agar well diffusion method.
26724686	7	37	theme	human	843:847	arg1	Proteus					1017:1023	Proteus	1017:1023	Proteus	1017:1023	Further the sulfated polysaccharide was evaluated for its antibacterial activity against selected human clinical pathogens, namely Staphylococcus aureus, Klebsiella pneumoniae, Salmonella typhi, Vibrio cholerae, Klebsiella oxytoca, Escherichia coli, Salmonella paratyphi, Proteus mirabilis, Vibrio parahaemolyticus and Streptococcus pyogenes using agar well diffusion method.
26724686	7	37	theme	human	843:847	arg1	Vibrio					940:945	Vibrio	940:945	Vibrio	940:945	Further the sulfated polysaccharide was evaluated for its antibacterial activity against selected human clinical pathogens, namely Staphylococcus aureus, Klebsiella pneumoniae, Salmonella typhi, Vibrio cholerae, Klebsiella oxytoca, Escherichia coli, Salmonella paratyphi, Proteus mirabilis, Vibrio parahaemolyticus and Streptococcus pyogenes using agar well diffusion method.
26724686	7	37	theme	human	843:847	arg1	pyogenes					1078:1085	Streptococcus pyogenes	1064:1085	Streptococcus pyogenes using agar well diffusion method	1064:1118	Further the sulfated polysaccharide was evaluated for its antibacterial activity against selected human clinical pathogens, namely Staphylococcus aureus, Klebsiella pneumoniae, Salmonella typhi, Vibrio cholerae, Klebsiella oxytoca, Escherichia coli, Salmonella paratyphi, Proteus mirabilis, Vibrio parahaemolyticus and Streptococcus pyogenes using agar well diffusion method.
26724686	7	37	theme	human	843:847	arg1	pathogens					858:866	selected human clinical pathogens	834:866	selected human clinical pathogens	834:866	Further the sulfated polysaccharide was evaluated for its antibacterial activity against selected human clinical pathogens, namely Staphylococcus aureus, Klebsiella pneumoniae, Salmonella typhi, Vibrio cholerae, Klebsiella oxytoca, Escherichia coli, Salmonella paratyphi, Proteus mirabilis, Vibrio parahaemolyticus and Streptococcus pyogenes using agar well diffusion method.
26724686	7	37	theme	human	843:847	arg1	coli					989:992	Escherichia coli	977:992	Escherichia coli	977:992	Further the sulfated polysaccharide was evaluated for its antibacterial activity against selected human clinical pathogens, namely Staphylococcus aureus, Klebsiella pneumoniae, Salmonella typhi, Vibrio cholerae, Klebsiella oxytoca, Escherichia coli, Salmonella paratyphi, Proteus mirabilis, Vibrio parahaemolyticus and Streptococcus pyogenes using agar well diffusion method.
26724686	7	37	theme	human	843:847	arg1	Klebsiella					899:908	Klebsiella	899:908	Klebsiella	899:908	Further the sulfated polysaccharide was evaluated for its antibacterial activity against selected human clinical pathogens, namely Staphylococcus aureus, Klebsiella pneumoniae, Salmonella typhi, Vibrio cholerae, Klebsiella oxytoca, Escherichia coli, Salmonella paratyphi, Proteus mirabilis, Vibrio parahaemolyticus and Streptococcus pyogenes using agar well diffusion method.
26724686	7	37	theme	human	843:847	arg1	parahaemolyticus					1043:1058	Vibrio parahaemolyticus	1036:1058	Vibrio parahaemolyticus	1036:1058	Further the sulfated polysaccharide was evaluated for its antibacterial activity against selected human clinical pathogens, namely Staphylococcus aureus, Klebsiella pneumoniae, Salmonella typhi, Vibrio cholerae, Klebsiella oxytoca, Escherichia coli, Salmonella paratyphi, Proteus mirabilis, Vibrio parahaemolyticus and Streptococcus pyogenes using agar well diffusion method.
26724686	7	37	theme	human	843:847	arg1	oxytoca					968:974	Klebsiella oxytoca	957:974	Klebsiella oxytoca	957:974	Further the sulfated polysaccharide was evaluated for its antibacterial activity against selected human clinical pathogens, namely Staphylococcus aureus, Klebsiella pneumoniae, Salmonella typhi, Vibrio cholerae, Klebsiella oxytoca, Escherichia coli, Salmonella paratyphi, Proteus mirabilis, Vibrio parahaemolyticus and Streptococcus pyogenes using agar well diffusion method.
26724686	7	37	theme	human	843:847	arg1	paratyphi					1006:1014	Salmonella paratyphi	995:1014	Salmonella paratyphi	995:1014	Further the sulfated polysaccharide was evaluated for its antibacterial activity against selected human clinical pathogens, namely Staphylococcus aureus, Klebsiella pneumoniae, Salmonella typhi, Vibrio cholerae, Klebsiella oxytoca, Escherichia coli, Salmonella paratyphi, Proteus mirabilis, Vibrio parahaemolyticus and Streptococcus pyogenes using agar well diffusion method.
26724686	7	37	theme	human	843:847	arg1	aureus					891:896	Staphylococcus aureus	876:896	Staphylococcus aureus	876:896	Further the sulfated polysaccharide was evaluated for its antibacterial activity against selected human clinical pathogens, namely Staphylococcus aureus, Klebsiella pneumoniae, Salmonella typhi, Vibrio cholerae, Klebsiella oxytoca, Escherichia coli, Salmonella paratyphi, Proteus mirabilis, Vibrio parahaemolyticus and Streptococcus pyogenes using agar well diffusion method.
26724686	7	38	theme	selected	834:841	arg1	typhi					933:937	Salmonella typhi	922:937	Salmonella typhi	922:937	Further the sulfated polysaccharide was evaluated for its antibacterial activity against selected human clinical pathogens, namely Staphylococcus aureus, Klebsiella pneumoniae, Salmonella typhi, Vibrio cholerae, Klebsiella oxytoca, Escherichia coli, Salmonella paratyphi, Proteus mirabilis, Vibrio parahaemolyticus and Streptococcus pyogenes using agar well diffusion method.
26724686	7	38	theme	selected	834:841	arg1	Proteus					1017:1023	Proteus	1017:1023	Proteus	1017:1023	Further the sulfated polysaccharide was evaluated for its antibacterial activity against selected human clinical pathogens, namely Staphylococcus aureus, Klebsiella pneumoniae, Salmonella typhi, Vibrio cholerae, Klebsiella oxytoca, Escherichia coli, Salmonella paratyphi, Proteus mirabilis, Vibrio parahaemolyticus and Streptococcus pyogenes using agar well diffusion method.
26724686	7	38	theme	selected	834:841	arg1	Vibrio					940:945	Vibrio	940:945	Vibrio	940:945	Further the sulfated polysaccharide was evaluated for its antibacterial activity against selected human clinical pathogens, namely Staphylococcus aureus, Klebsiella pneumoniae, Salmonella typhi, Vibrio cholerae, Klebsiella oxytoca, Escherichia coli, Salmonella paratyphi, Proteus mirabilis, Vibrio parahaemolyticus and Streptococcus pyogenes using agar well diffusion method.
26724686	7	38	theme	selected	834:841	arg1	pyogenes					1078:1085	Streptococcus pyogenes	1064:1085	Streptococcus pyogenes using agar well diffusion method	1064:1118	Further the sulfated polysaccharide was evaluated for its antibacterial activity against selected human clinical pathogens, namely Staphylococcus aureus, Klebsiella pneumoniae, Salmonella typhi, Vibrio cholerae, Klebsiella oxytoca, Escherichia coli, Salmonella paratyphi, Proteus mirabilis, Vibrio parahaemolyticus and Streptococcus pyogenes using agar well diffusion method.
26724686	7	38	theme	selected	834:841	arg1	pathogens					858:866	selected human clinical pathogens	834:866	selected human clinical pathogens	834:866	Further the sulfated polysaccharide was evaluated for its antibacterial activity against selected human clinical pathogens, namely Staphylococcus aureus, Klebsiella pneumoniae, Salmonella typhi, Vibrio cholerae, Klebsiella oxytoca, Escherichia coli, Salmonella paratyphi, Proteus mirabilis, Vibrio parahaemolyticus and Streptococcus pyogenes using agar well diffusion method.
26724686	7	38	theme	selected	834:841	arg1	coli					989:992	Escherichia coli	977:992	Escherichia coli	977:992	Further the sulfated polysaccharide was evaluated for its antibacterial activity against selected human clinical pathogens, namely Staphylococcus aureus, Klebsiella pneumoniae, Salmonella typhi, Vibrio cholerae, Klebsiella oxytoca, Escherichia coli, Salmonella paratyphi, Proteus mirabilis, Vibrio parahaemolyticus and Streptococcus pyogenes using agar well diffusion method.
26724686	7	38	theme	selected	834:841	arg1	Klebsiella					899:908	Klebsiella	899:908	Klebsiella	899:908	Further the sulfated polysaccharide was evaluated for its antibacterial activity against selected human clinical pathogens, namely Staphylococcus aureus, Klebsiella pneumoniae, Salmonella typhi, Vibrio cholerae, Klebsiella oxytoca, Escherichia coli, Salmonella paratyphi, Proteus mirabilis, Vibrio parahaemolyticus and Streptococcus pyogenes using agar well diffusion method.
26724686	7	38	theme	selected	834:841	arg1	parahaemolyticus					1043:1058	Vibrio parahaemolyticus	1036:1058	Vibrio parahaemolyticus	1036:1058	Further the sulfated polysaccharide was evaluated for its antibacterial activity against selected human clinical pathogens, namely Staphylococcus aureus, Klebsiella pneumoniae, Salmonella typhi, Vibrio cholerae, Klebsiella oxytoca, Escherichia coli, Salmonella paratyphi, Proteus mirabilis, Vibrio parahaemolyticus and Streptococcus pyogenes using agar well diffusion method.
26724686	7	38	theme	selected	834:841	arg1	oxytoca					968:974	Klebsiella oxytoca	957:974	Klebsiella oxytoca	957:974	Further the sulfated polysaccharide was evaluated for its antibacterial activity against selected human clinical pathogens, namely Staphylococcus aureus, Klebsiella pneumoniae, Salmonella typhi, Vibrio cholerae, Klebsiella oxytoca, Escherichia coli, Salmonella paratyphi, Proteus mirabilis, Vibrio parahaemolyticus and Streptococcus pyogenes using agar well diffusion method.
26724686	7	38	theme	selected	834:841	arg1	paratyphi					1006:1014	Salmonella paratyphi	995:1014	Salmonella paratyphi	995:1014	Further the sulfated polysaccharide was evaluated for its antibacterial activity against selected human clinical pathogens, namely Staphylococcus aureus, Klebsiella pneumoniae, Salmonella typhi, Vibrio cholerae, Klebsiella oxytoca, Escherichia coli, Salmonella paratyphi, Proteus mirabilis, Vibrio parahaemolyticus and Streptococcus pyogenes using agar well diffusion method.
26724686	7	38	theme	selected	834:841	arg1	aureus					891:896	Staphylococcus aureus	876:896	Staphylococcus aureus	876:896	Further the sulfated polysaccharide was evaluated for its antibacterial activity against selected human clinical pathogens, namely Staphylococcus aureus, Klebsiella pneumoniae, Salmonella typhi, Vibrio cholerae, Klebsiella oxytoca, Escherichia coli, Salmonella paratyphi, Proteus mirabilis, Vibrio parahaemolyticus and Streptococcus pyogenes using agar well diffusion method.
26724686	7	39	theme	clinical	849:856	arg1	typhi					933:937	Salmonella typhi	922:937	Salmonella typhi	922:937	Further the sulfated polysaccharide was evaluated for its antibacterial activity against selected human clinical pathogens, namely Staphylococcus aureus, Klebsiella pneumoniae, Salmonella typhi, Vibrio cholerae, Klebsiella oxytoca, Escherichia coli, Salmonella paratyphi, Proteus mirabilis, Vibrio parahaemolyticus and Streptococcus pyogenes using agar well diffusion method.
26724686	7	39	theme	clinical	849:856	arg1	Proteus					1017:1023	Proteus	1017:1023	Proteus	1017:1023	Further the sulfated polysaccharide was evaluated for its antibacterial activity against selected human clinical pathogens, namely Staphylococcus aureus, Klebsiella pneumoniae, Salmonella typhi, Vibrio cholerae, Klebsiella oxytoca, Escherichia coli, Salmonella paratyphi, Proteus mirabilis, Vibrio parahaemolyticus and Streptococcus pyogenes using agar well diffusion method.
26724686	7	39	theme	clinical	849:856	arg1	Vibrio					940:945	Vibrio	940:945	Vibrio	940:945	Further the sulfated polysaccharide was evaluated for its antibacterial activity against selected human clinical pathogens, namely Staphylococcus aureus, Klebsiella pneumoniae, Salmonella typhi, Vibrio cholerae, Klebsiella oxytoca, Escherichia coli, Salmonella paratyphi, Proteus mirabilis, Vibrio parahaemolyticus and Streptococcus pyogenes using agar well diffusion method.
26724686	7	39	theme	clinical	849:856	arg1	pyogenes					1078:1085	Streptococcus pyogenes	1064:1085	Streptococcus pyogenes using agar well diffusion method	1064:1118	Further the sulfated polysaccharide was evaluated for its antibacterial activity against selected human clinical pathogens, namely Staphylococcus aureus, Klebsiella pneumoniae, Salmonella typhi, Vibrio cholerae, Klebsiella oxytoca, Escherichia coli, Salmonella paratyphi, Proteus mirabilis, Vibrio parahaemolyticus and Streptococcus pyogenes using agar well diffusion method.
26724686	7	39	theme	clinical	849:856	arg1	pathogens					858:866	selected human clinical pathogens	834:866	selected human clinical pathogens	834:866	Further the sulfated polysaccharide was evaluated for its antibacterial activity against selected human clinical pathogens, namely Staphylococcus aureus, Klebsiella pneumoniae, Salmonella typhi, Vibrio cholerae, Klebsiella oxytoca, Escherichia coli, Salmonella paratyphi, Proteus mirabilis, Vibrio parahaemolyticus and Streptococcus pyogenes using agar well diffusion method.
26724686	7	39	theme	clinical	849:856	arg1	coli					989:992	Escherichia coli	977:992	Escherichia coli	977:992	Further the sulfated polysaccharide was evaluated for its antibacterial activity against selected human clinical pathogens, namely Staphylococcus aureus, Klebsiella pneumoniae, Salmonella typhi, Vibrio cholerae, Klebsiella oxytoca, Escherichia coli, Salmonella paratyphi, Proteus mirabilis, Vibrio parahaemolyticus and Streptococcus pyogenes using agar well diffusion method.
26724686	7	39	theme	clinical	849:856	arg1	Klebsiella					899:908	Klebsiella	899:908	Klebsiella	899:908	Further the sulfated polysaccharide was evaluated for its antibacterial activity against selected human clinical pathogens, namely Staphylococcus aureus, Klebsiella pneumoniae, Salmonella typhi, Vibrio cholerae, Klebsiella oxytoca, Escherichia coli, Salmonella paratyphi, Proteus mirabilis, Vibrio parahaemolyticus and Streptococcus pyogenes using agar well diffusion method.
26724686	7	39	theme	clinical	849:856	arg1	parahaemolyticus					1043:1058	Vibrio parahaemolyticus	1036:1058	Vibrio parahaemolyticus	1036:1058	Further the sulfated polysaccharide was evaluated for its antibacterial activity against selected human clinical pathogens, namely Staphylococcus aureus, Klebsiella pneumoniae, Salmonella typhi, Vibrio cholerae, Klebsiella oxytoca, Escherichia coli, Salmonella paratyphi, Proteus mirabilis, Vibrio parahaemolyticus and Streptococcus pyogenes using agar well diffusion method.
26724686	7	39	theme	clinical	849:856	arg1	oxytoca					968:974	Klebsiella oxytoca	957:974	Klebsiella oxytoca	957:974	Further the sulfated polysaccharide was evaluated for its antibacterial activity against selected human clinical pathogens, namely Staphylococcus aureus, Klebsiella pneumoniae, Salmonella typhi, Vibrio cholerae, Klebsiella oxytoca, Escherichia coli, Salmonella paratyphi, Proteus mirabilis, Vibrio parahaemolyticus and Streptococcus pyogenes using agar well diffusion method.
26724686	7	39	theme	clinical	849:856	arg1	paratyphi					1006:1014	Salmonella paratyphi	995:1014	Salmonella paratyphi	995:1014	Further the sulfated polysaccharide was evaluated for its antibacterial activity against selected human clinical pathogens, namely Staphylococcus aureus, Klebsiella pneumoniae, Salmonella typhi, Vibrio cholerae, Klebsiella oxytoca, Escherichia coli, Salmonella paratyphi, Proteus mirabilis, Vibrio parahaemolyticus and Streptococcus pyogenes using agar well diffusion method.
26724686	7	39	theme	clinical	849:856	arg1	aureus					891:896	Staphylococcus aureus	876:896	Staphylococcus aureus	876:896	Further the sulfated polysaccharide was evaluated for its antibacterial activity against selected human clinical pathogens, namely Staphylococcus aureus, Klebsiella pneumoniae, Salmonella typhi, Vibrio cholerae, Klebsiella oxytoca, Escherichia coli, Salmonella paratyphi, Proteus mirabilis, Vibrio parahaemolyticus and Streptococcus pyogenes using agar well diffusion method.
26724686	7	40	theme	antibacterial	803:815	arg1	activity					817:824	its antibacterial activity	799:824	its antibacterial activity against selected human clinical pathogens, namely Staphylococcus aureus, Klebsiella pneumoniae, Salmonella typhi, Vibrio cholerae, Klebsiella oxytoca, Escherichia coli, Salmonella paratyphi, Proteus mirabilis, Vibrio parahaemolyticus and Streptococcus pyogenes using agar well diffusion method	799:1118	Further the sulfated polysaccharide was evaluated for its antibacterial activity against selected human clinical pathogens, namely Staphylococcus aureus, Klebsiella pneumoniae, Salmonella typhi, Vibrio cholerae, Klebsiella oxytoca, Escherichia coli, Salmonella paratyphi, Proteus mirabilis, Vibrio parahaemolyticus and Streptococcus pyogenes using agar well diffusion method.
26724686	0	41	from	gladius	90:96	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and biomedical properties of sulfated polysaccharide from the gladius of Sepioteuthis lessoniana (Lesson, 1831).
26724686	0	41	from	gladius	90:96	arg1	properties					43:52	biomedical properties	32:52	biomedical properties	32:52	Structural characterization and biomedical properties of sulfated polysaccharide from the gladius of Sepioteuthis lessoniana (Lesson, 1831).
26724686	0	41	from	gladius	90:96	arg1	polysaccharide					66:79	sulfated polysaccharide	57:79	sulfated polysaccharide from the gladius of Sepioteuthis lessoniana (Lesson, 1831)	57:138	Structural characterization and biomedical properties of sulfated polysaccharide from the gladius of Sepioteuthis lessoniana (Lesson, 1831).
26724686	6	42	theme	structural	653:662	arg1	features					664:671	The structural features	649:671	The structural features of sulfated polysaccharide	649:698	The structural features of sulfated polysaccharide were analyzed by FT-IR and NMR spectroscopy.
26724686	7	43	theme	Vibrio	1036:1041	arg1	pathogens					858:866	selected human clinical pathogens	834:866	selected human clinical pathogens	834:866	Further the sulfated polysaccharide was evaluated for its antibacterial activity against selected human clinical pathogens, namely Staphylococcus aureus, Klebsiella pneumoniae, Salmonella typhi, Vibrio cholerae, Klebsiella oxytoca, Escherichia coli, Salmonella paratyphi, Proteus mirabilis, Vibrio parahaemolyticus and Streptococcus pyogenes using agar well diffusion method.
26724686	7	43	theme	Vibrio	1036:1041	arg1	parahaemolyticus					1043:1058	Vibrio parahaemolyticus	1036:1058	Vibrio parahaemolyticus	1036:1058	Further the sulfated polysaccharide was evaluated for its antibacterial activity against selected human clinical pathogens, namely Staphylococcus aureus, Klebsiella pneumoniae, Salmonella typhi, Vibrio cholerae, Klebsiella oxytoca, Escherichia coli, Salmonella paratyphi, Proteus mirabilis, Vibrio parahaemolyticus and Streptococcus pyogenes using agar well diffusion method.
26724686	0	44	theme	polysaccharide	66:79	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and biomedical properties of sulfated polysaccharide from the gladius of Sepioteuthis lessoniana (Lesson, 1831).
26724686	0	44	theme	polysaccharide	66:79	arg1	properties					43:52	biomedical properties	32:52	biomedical properties	32:52	Structural characterization and biomedical properties of sulfated polysaccharide from the gladius of Sepioteuthis lessoniana (Lesson, 1831).
26724686	8	45	theme	good	1151:1154	arg1	activity					1170:1177	good antibacterial activity	1151:1177	good antibacterial activity	1151:1177	The polysaccharide has showed good antibacterial activity and MIC and MBC have also been evaluated.
26724686	4	46	theme	molecular	434:442	arg1	weight					444:449	The molecular weight	430:449	The molecular weight of sulfated polysaccharide determined through PAGE	430:500	The molecular weight of sulfated polysaccharide determined through PAGE was found to be as 66 kDa.
26724686	11	47	theme	substance	1572:1580	arg1	source					1562:1567	an alternate, non-toxic and cheap source	1528:1567	an alternate, non-toxic and cheap source of substance that showed good antibacterial and anticancer acitivity	1528:1636	In conclusion, the sulfated polysaccharide is an alternate, non-toxic and cheap source of substance that showed good antibacterial and anticancer acitivity.
26724686	11	47	theme	substance	1572:1580	arg1	polysaccharide					1510:1523	the sulfated polysaccharide	1497:1523	the sulfated polysaccharide	1497:1523	In conclusion, the sulfated polysaccharide is an alternate, non-toxic and cheap source of substance that showed good antibacterial and anticancer acitivity.
26724686	0	48	theme	sulfated	57:64	arg1	polysaccharide					66:79	sulfated polysaccharide	57:79	sulfated polysaccharide from the gladius of Sepioteuthis lessoniana (Lesson, 1831)	57:138	Structural characterization and biomedical properties of sulfated polysaccharide from the gladius of Sepioteuthis lessoniana (Lesson, 1831).
26724686	11	49	from	source	1562:1567	arg1	conclusion					1485:1494	conclusion	1485:1494	conclusion	1485:1494	In conclusion, the sulfated polysaccharide is an alternate, non-toxic and cheap source of substance that showed good antibacterial and anticancer acitivity.
26724686	5	50	theme	Monosaccharides	529:543	arg1	analysis					545:552	Monosaccharides analysis	529:552	Monosaccharides analysis	529:552	Monosaccharides analysis revealed that sulfated polysaccharide was composed of rhamnose, galactose, xylose and glucose.
26724686	7	51	dep	Proteus	1017:1023	arg1	mirabilis					1025:1033	mirabilis	1025:1033	mirabilis	1025:1033	Further the sulfated polysaccharide was evaluated for its antibacterial activity against selected human clinical pathogens, namely Staphylococcus aureus, Klebsiella pneumoniae, Salmonella typhi, Vibrio cholerae, Klebsiella oxytoca, Escherichia coli, Salmonella paratyphi, Proteus mirabilis, Vibrio parahaemolyticus and Streptococcus pyogenes using agar well diffusion method.
26724686	1	52	theme	Sepioteuthis	216:227	arg1	lessoniana					229:238	Sepioteuthis lessoniana	216:238	Sepioteuthis lessoniana	216:238	Sulfated polysaccharide was extracted from the internal shell (gladius) of Sepioteuthis lessoniana.
26724686	8	53	theme	antibacterial	1156:1168	arg1	activity					1170:1177	good antibacterial activity	1151:1177	good antibacterial activity	1151:1177	The polysaccharide has showed good antibacterial activity and MIC and MBC have also been evaluated.
26724686	10	54	theme	maximum	1362:1368	arg1	activity					1381:1388	the maximum anticancer activity	1358:1388	the maximum anticancer activity of 62.89%	1358:1398	The Cytotoxic Concentration (CC50) was observed as 700 μg/ml and the maximum anticancer activity of 62.89% was recorded at 200 μg/ml; whereas, the lowest of 9.87% was observed at 25 μg/ml.
26724686	11	55	theme	good	1594:1597	arg1	acitivity					1628:1636	good antibacterial and anticancer acitivity	1594:1636	good antibacterial and anticancer acitivity	1594:1636	In conclusion, the sulfated polysaccharide is an alternate, non-toxic and cheap source of substance that showed good antibacterial and anticancer acitivity.
26724686	7	56	theme	Salmonella	922:931	arg1	typhi					933:937	Salmonella typhi	922:937	Salmonella typhi	922:937	Further the sulfated polysaccharide was evaluated for its antibacterial activity against selected human clinical pathogens, namely Staphylococcus aureus, Klebsiella pneumoniae, Salmonella typhi, Vibrio cholerae, Klebsiella oxytoca, Escherichia coli, Salmonella paratyphi, Proteus mirabilis, Vibrio parahaemolyticus and Streptococcus pyogenes using agar well diffusion method.
26724686	7	56	theme	Salmonella	922:931	arg1	pathogens					858:866	selected human clinical pathogens	834:866	selected human clinical pathogens	834:866	Further the sulfated polysaccharide was evaluated for its antibacterial activity against selected human clinical pathogens, namely Staphylococcus aureus, Klebsiella pneumoniae, Salmonella typhi, Vibrio cholerae, Klebsiella oxytoca, Escherichia coli, Salmonella paratyphi, Proteus mirabilis, Vibrio parahaemolyticus and Streptococcus pyogenes using agar well diffusion method.
26724686	1	57	theme	lessoniana	229:238	arg1	gladius					204:210	gladius	204:210	gladius	204:210	Sulfated polysaccharide was extracted from the internal shell (gladius) of Sepioteuthis lessoniana.
26724686	1	57	theme	lessoniana	229:238	arg1	shell					197:201	the internal shell	184:201	the internal shell (gladius) of Sepioteuthis lessoniana	184:238	Sulfated polysaccharide was extracted from the internal shell (gladius) of Sepioteuthis lessoniana.
26724686	7	58	dep	agar	1093:1096	arg1	method					1113:1118	diffusion method	1103:1118	diffusion method	1103:1118	Further the sulfated polysaccharide was evaluated for its antibacterial activity against selected human clinical pathogens, namely Staphylococcus aureus, Klebsiella pneumoniae, Salmonella typhi, Vibrio cholerae, Klebsiella oxytoca, Escherichia coli, Salmonella paratyphi, Proteus mirabilis, Vibrio parahaemolyticus and Streptococcus pyogenes using agar well diffusion method.
26724686	6	59	theme	NMR	727:729	arg1	spectroscopy					731:742	FT-IR and NMR spectroscopy	717:742	spectroscopy	731:742	The structural features of sulfated polysaccharide were analyzed by FT-IR and NMR spectroscopy.
26724686	1	60	theme	Sulfated	141:148	arg1	polysaccharide					150:163	Sulfated polysaccharide	141:163	Sulfated polysaccharide	141:163	Sulfated polysaccharide was extracted from the internal shell (gladius) of Sepioteuthis lessoniana.
26724686	2	61	theme	sulfated	245:252	arg1	polysaccharide					254:267	The sulfated polysaccharide	241:267	The sulfated polysaccharide	241:267	The sulfated polysaccharide contained 61.3% of carbohydrate, 0.8% of protein, 28.2% of ash and 1.33% of moisture respectively.
26724686	10	62	theme	Cytotoxic	1297:1305	arg1	CC50					1322:1325	CC50	1322:1325	CC50	1322:1325	The Cytotoxic Concentration (CC50) was observed as 700 μg/ml and the maximum anticancer activity of 62.89% was recorded at 200 μg/ml; whereas, the lowest of 9.87% was observed at 25 μg/ml.
26724686	10	62	theme	Cytotoxic	1297:1305	arg1	Concentration					1307:1319	The Cytotoxic Concentration	1293:1319	The Cytotoxic Concentration (CC50)	1293:1326	The Cytotoxic Concentration (CC50) was observed as 700 μg/ml and the maximum anticancer activity of 62.89% was recorded at 200 μg/ml; whereas, the lowest of 9.87% was observed at 25 μg/ml.
26724686	10	62	theme	Cytotoxic	1297:1305	arg1	μg/ml					1348:1352	700 μg/ml	1344:1352	700 μg/ml	1344:1352	The Cytotoxic Concentration (CC50) was observed as 700 μg/ml and the maximum anticancer activity of 62.89% was recorded at 200 μg/ml; whereas, the lowest of 9.87% was observed at 25 μg/ml.
25157382	2	0	from	stress	431:436	arg1	mice					441:444	mice	441:444	mice fed a high-fructose diet	441:469	The present study investigates the preventive effects of Pleurotus eryngii polysaccharides (PEP), which showed powerful antioxidant activity in vitro, on insulin resistance and oxidative stress in mice fed a high-fructose diet.
25157382	3	1	with	heteropolysaccharides	506:526	arg1	d-galactose					558:568	d-galactose	558:568	d-galactose (24.4%)	558:576	PEP was identified by HPLC as the heteropolysaccharides with d-glucose (62.8%, mol%), d-galactose (24.4%) and d-mannose (9.8%) being the main component monosaccharides.
25157382	3	1	with	heteropolysaccharides	506:526	arg1	d-mannose					582:590	d-mannose	582:590	d-mannose	582:590	PEP was identified by HPLC as the heteropolysaccharides with d-glucose (62.8%, mol%), d-galactose (24.4%) and d-mannose (9.8%) being the main component monosaccharides.
25157382	3	1	with	heteropolysaccharides	506:526	arg1	d-glucose					533:541	d-glucose	533:541	d-glucose (62.8%, mol%)	533:555	PEP was identified by HPLC as the heteropolysaccharides with d-glucose (62.8%, mol%), d-galactose (24.4%) and d-mannose (9.8%) being the main component monosaccharides.
25157382	2	2	theme	antioxidant	364:374	arg1	activity					376:383	powerful antioxidant activity	355:383	powerful antioxidant activity	355:383	The present study investigates the preventive effects of Pleurotus eryngii polysaccharides (PEP), which showed powerful antioxidant activity in vitro, on insulin resistance and oxidative stress in mice fed a high-fructose diet.
25157382	2	3	theme	Pleurotus	301:309	arg1	PEP					336:338	PEP	336:338	PEP	336:338	The present study investigates the preventive effects of Pleurotus eryngii polysaccharides (PEP), which showed powerful antioxidant activity in vitro, on insulin resistance and oxidative stress in mice fed a high-fructose diet.
25157382	2	3	theme	Pleurotus	301:309	arg1	polysaccharides					319:333	Pleurotus eryngii polysaccharides	301:333	Pleurotus eryngii polysaccharides (PEP)	301:339	The present study investigates the preventive effects of Pleurotus eryngii polysaccharides (PEP), which showed powerful antioxidant activity in vitro, on insulin resistance and oxidative stress in mice fed a high-fructose diet.
25157382	4	4	theme	insulin	804:810	arg1	sensitivity					812:822	impaired insulin sensitivity	795:822	impaired insulin sensitivity (p < 0.05)	795:833	Mice fed 20% fructose in drinking water for 6 weeks significantly displayed hyperglycemia, hyperinsulinemia, dyslipidemia and liver oxidative stress with impaired insulin sensitivity (p < 0.05).
25157382	4	4	theme	insulin	804:810	arg1	<					827:827	p < 0.05	825:832	p < 0.05	825:832	Mice fed 20% fructose in drinking water for 6 weeks significantly displayed hyperglycemia, hyperinsulinemia, dyslipidemia and liver oxidative stress with impaired insulin sensitivity (p < 0.05).
25157382	6	5	theme	red	1168:1170	arg1	staining					1174:1181	conventional H&E and oil red O staining	1143:1181	staining	1174:1181	The histopathology of the liver by conventional H&E and oil red O staining confirmed the liver steatosis induced by a HF diet and the hepatoprotective effect of PEP.
25157382	1	6	theme	fructose	189:196	arg1	intake					171:176	High intake	166:176	High intake of dietary fructose	166:196	High intake of dietary fructose exerts a number of adverse metabolic effects.
25157382	0	7	theme	oxidative	140:148	arg1	stress					150:155	oxidative stress	140:155	oxidative stress	140:155	Chemical composition of Pleurotus eryngii polysaccharides and their inhibitory effects on high-fructose diet-induced insulin resistance and oxidative stress in mice.
25157382	5	8	theme	HF-fed	989:994	arg1	mice					996:999	HF-fed mice	989:999	HF-fed mice	989:999	The administration of PEP at 400 and 800 mg kg(-1) bw significantly reduced the fasting serum glucose and insulin concentrations and lipid deposition in HF-fed mice, and caused the reduction of liver lipid peroxidation and the elevation of the hepatic antioxidant system.
25157382	2	9	theme	polysaccharides	319:333	arg1	effects					290:296	the preventive effects	275:296	the preventive effects of Pleurotus eryngii polysaccharides (PEP), which showed powerful antioxidant activity in vitro, on insulin resistance and oxidative stress in mice fed a high-fructose diet	275:469	The present study investigates the preventive effects of Pleurotus eryngii polysaccharides (PEP), which showed powerful antioxidant activity in vitro, on insulin resistance and oxidative stress in mice fed a high-fructose diet.
25157382	6	10	theme	HF	1226:1227	arg1	diet					1229:1232	a HF diet	1224:1232	a HF diet	1224:1232	The histopathology of the liver by conventional H&E and oil red O staining confirmed the liver steatosis induced by a HF diet and the hepatoprotective effect of PEP.
25157382	1	11	theme	metabolic	225:233	arg1	effects					235:241	adverse metabolic effects	217:241	adverse metabolic effects	217:241	High intake of dietary fructose exerts a number of adverse metabolic effects.
25157382	0	12	from	resistance	125:134	arg1	composition					9:19	Chemical composition	0:19	Chemical composition of Pleurotus eryngii polysaccharides and their inhibitory effects on high-fructose diet-induced insulin resistance and oxidative stress in mice.	0:164	Chemical composition of Pleurotus eryngii polysaccharides and their inhibitory effects on high-fructose diet-induced insulin resistance and oxidative stress in mice.
25157382	4	13	theme	drinking	666:673	arg1	water					675:679	drinking water	666:679	drinking water	666:679	Mice fed 20% fructose in drinking water for 6 weeks significantly displayed hyperglycemia, hyperinsulinemia, dyslipidemia and liver oxidative stress with impaired insulin sensitivity (p < 0.05).
25157382	7	14	theme	liver	1492:1496	arg1	dysfunction					1498:1508	liver dysfunction	1492:1508	liver dysfunction	1492:1508	These results suggest that Pleurotus eryngii is a potential source of polysaccharides, and might be regarded as a novel preventive and therapeutic product for the mitigation of insulin resistance, oxidative stress and liver dysfunction.
25157382	7	14	theme	liver	1492:1496	arg1	product					1421:1427	a novel preventive and therapeutic product	1386:1427	a novel preventive and therapeutic product for the mitigation of insulin resistance	1386:1468	These results suggest that Pleurotus eryngii is a potential source of polysaccharides, and might be regarded as a novel preventive and therapeutic product for the mitigation of insulin resistance, oxidative stress and liver dysfunction.
25157382	0	15	theme	inhibitory	68:77	arg1	effects					79:85	their inhibitory effects	62:85	their inhibitory effects on high-fructose diet-induced insulin resistance and oxidative stress in mice	62:163	Chemical composition of Pleurotus eryngii polysaccharides and their inhibitory effects on high-fructose diet-induced insulin resistance and oxidative stress in mice.
25157382	5	16	theme	liver	1030:1034	arg1	peroxidation					1042:1053	liver lipid peroxidation	1030:1053	liver lipid peroxidation	1030:1053	The administration of PEP at 400 and 800 mg kg(-1) bw significantly reduced the fasting serum glucose and insulin concentrations and lipid deposition in HF-fed mice, and caused the reduction of liver lipid peroxidation and the elevation of the hepatic antioxidant system.
25157382	4	17	dep	hyperglycemia	717:729	arg1	stress					783:788	oxidative stress	773:788	oxidative stress	773:788	Mice fed 20% fructose in drinking water for 6 weeks significantly displayed hyperglycemia, hyperinsulinemia, dyslipidemia and liver oxidative stress with impaired insulin sensitivity (p < 0.05).
25157382	4	18	theme	20	650:651	arg1	%					652:652	%	652:652	%	652:652	Mice fed 20% fructose in drinking water for 6 weeks significantly displayed hyperglycemia, hyperinsulinemia, dyslipidemia and liver oxidative stress with impaired insulin sensitivity (p < 0.05).
25157382	5	19	theme	peroxidation	1042:1053	arg1	reduction					1017:1025	the reduction	1013:1025	the reduction of liver lipid peroxidation	1013:1053	The administration of PEP at 400 and 800 mg kg(-1) bw significantly reduced the fasting serum glucose and insulin concentrations and lipid deposition in HF-fed mice, and caused the reduction of liver lipid peroxidation and the elevation of the hepatic antioxidant system.
25157382	5	19	theme	peroxidation	1042:1053	arg1	elevation					1063:1071	the elevation	1059:1071	the elevation of the hepatic antioxidant system	1059:1105	The administration of PEP at 400 and 800 mg kg(-1) bw significantly reduced the fasting serum glucose and insulin concentrations and lipid deposition in HF-fed mice, and caused the reduction of liver lipid peroxidation and the elevation of the hepatic antioxidant system.
25157382	0	20	theme	diet-induced	104:115	arg1	resistance					125:134	high-fructose diet-induced insulin resistance	90:134	high-fructose diet-induced insulin resistance	90:134	Chemical composition of Pleurotus eryngii polysaccharides and their inhibitory effects on high-fructose diet-induced insulin resistance and oxidative stress in mice.
25157382	7	21	theme	insulin	1451:1457	arg1	resistance					1459:1468	insulin resistance	1451:1468	insulin resistance	1451:1468	These results suggest that Pleurotus eryngii is a potential source of polysaccharides, and might be regarded as a novel preventive and therapeutic product for the mitigation of insulin resistance, oxidative stress and liver dysfunction.
25157382	6	22	theme	hepatoprotective	1242:1257	arg1	effect					1259:1264	the hepatoprotective effect	1238:1264	the hepatoprotective effect of PEP	1238:1271	The histopathology of the liver by conventional H&E and oil red O staining confirmed the liver steatosis induced by a HF diet and the hepatoprotective effect of PEP.
25157382	2	23	theme	high-fructose	452:464	arg1	diet					466:469	a high-fructose diet	450:469	a high-fructose diet	450:469	The present study investigates the preventive effects of Pleurotus eryngii polysaccharides (PEP), which showed powerful antioxidant activity in vitro, on insulin resistance and oxidative stress in mice fed a high-fructose diet.
25157382	0	24	theme	effects	79:85	arg1	composition					9:19	Chemical composition	0:19	Chemical composition of Pleurotus eryngii polysaccharides and their inhibitory effects on high-fructose diet-induced insulin resistance and oxidative stress in mice.	0:164	Chemical composition of Pleurotus eryngii polysaccharides and their inhibitory effects on high-fructose diet-induced insulin resistance and oxidative stress in mice.
25157382	0	25	theme	Chemical	0:7	arg1	composition					9:19	Chemical composition	0:19	Chemical composition of Pleurotus eryngii polysaccharides and their inhibitory effects on high-fructose diet-induced insulin resistance and oxidative stress in mice.	0:164	Chemical composition of Pleurotus eryngii polysaccharides and their inhibitory effects on high-fructose diet-induced insulin resistance and oxidative stress in mice.
25157382	5	26	theme	hepatic	1080:1086	arg1	system					1100:1105	the hepatic antioxidant system	1076:1105	the hepatic antioxidant system	1076:1105	The administration of PEP at 400 and 800 mg kg(-1) bw significantly reduced the fasting serum glucose and insulin concentrations and lipid deposition in HF-fed mice, and caused the reduction of liver lipid peroxidation and the elevation of the hepatic antioxidant system.
25157382	5	27	theme	mg	877:878	arg1	kg					880:881	400 and 800 mg kg(-1)	865:885	400 and 800 mg kg(-1)	865:885	The administration of PEP at 400 and 800 mg kg(-1) bw significantly reduced the fasting serum glucose and insulin concentrations and lipid deposition in HF-fed mice, and caused the reduction of liver lipid peroxidation and the elevation of the hepatic antioxidant system.
25157382	5	28	theme	system	1100:1105	arg1	reduction					1017:1025	the reduction	1013:1025	the reduction of liver lipid peroxidation	1013:1053	The administration of PEP at 400 and 800 mg kg(-1) bw significantly reduced the fasting serum glucose and insulin concentrations and lipid deposition in HF-fed mice, and caused the reduction of liver lipid peroxidation and the elevation of the hepatic antioxidant system.
25157382	5	28	theme	system	1100:1105	arg1	elevation					1063:1071	the elevation	1059:1071	the elevation of the hepatic antioxidant system	1059:1105	The administration of PEP at 400 and 800 mg kg(-1) bw significantly reduced the fasting serum glucose and insulin concentrations and lipid deposition in HF-fed mice, and caused the reduction of liver lipid peroxidation and the elevation of the hepatic antioxidant system.
25157382	3	29	dep	d-glucose	533:541	arg1	%					548:548	62.8%	544:548	62.8%	544:548	PEP was identified by HPLC as the heteropolysaccharides with d-glucose (62.8%, mol%), d-galactose (24.4%) and d-mannose (9.8%) being the main component monosaccharides.
25157382	3	29	dep	d-glucose	533:541	arg1	%					554:554	mol%	551:554	mol%	551:554	PEP was identified by HPLC as the heteropolysaccharides with d-glucose (62.8%, mol%), d-galactose (24.4%) and d-mannose (9.8%) being the main component monosaccharides.
25157382	4	30	theme	oxidative	773:781	arg1	stress					783:788	oxidative stress	773:788	oxidative stress	773:788	Mice fed 20% fructose in drinking water for 6 weeks significantly displayed hyperglycemia, hyperinsulinemia, dyslipidemia and liver oxidative stress with impaired insulin sensitivity (p < 0.05).
25157382	2	31	theme	oxidative	421:429	arg1	stress					431:436	oxidative stress	421:436	oxidative stress	421:436	The present study investigates the preventive effects of Pleurotus eryngii polysaccharides (PEP), which showed powerful antioxidant activity in vitro, on insulin resistance and oxidative stress in mice fed a high-fructose diet.
25157382	2	32	theme	powerful	355:362	arg1	activity					376:383	powerful antioxidant activity	355:383	powerful antioxidant activity	355:383	The present study investigates the preventive effects of Pleurotus eryngii polysaccharides (PEP), which showed powerful antioxidant activity in vitro, on insulin resistance and oxidative stress in mice fed a high-fructose diet.
25157382	2	33	from	resistance	406:415	arg1	mice					441:444	mice	441:444	mice fed a high-fructose diet	441:469	The present study investigates the preventive effects of Pleurotus eryngii polysaccharides (PEP), which showed powerful antioxidant activity in vitro, on insulin resistance and oxidative stress in mice fed a high-fructose diet.
25157382	1	34	theme	effects	235:241	arg1	number					207:212	a number	205:212	a number of adverse metabolic effects	205:241	High intake of dietary fructose exerts a number of adverse metabolic effects.
25157382	7	35	theme	novel	1388:1392	arg1	eryngii					1311:1317	Pleurotus eryngii	1301:1317	Pleurotus eryngii	1301:1317	These results suggest that Pleurotus eryngii is a potential source of polysaccharides, and might be regarded as a novel preventive and therapeutic product for the mitigation of insulin resistance, oxidative stress and liver dysfunction.
25157382	7	35	theme	novel	1388:1392	arg1	stress					1481:1486	oxidative stress	1471:1486	oxidative stress	1471:1486	These results suggest that Pleurotus eryngii is a potential source of polysaccharides, and might be regarded as a novel preventive and therapeutic product for the mitigation of insulin resistance, oxidative stress and liver dysfunction.
25157382	7	35	theme	novel	1388:1392	arg1	dysfunction					1498:1508	liver dysfunction	1492:1508	liver dysfunction	1492:1508	These results suggest that Pleurotus eryngii is a potential source of polysaccharides, and might be regarded as a novel preventive and therapeutic product for the mitigation of insulin resistance, oxidative stress and liver dysfunction.
25157382	7	35	theme	novel	1388:1392	arg1	product					1421:1427	a novel preventive and therapeutic product	1386:1427	a novel preventive and therapeutic product for the mitigation of insulin resistance	1386:1468	These results suggest that Pleurotus eryngii is a potential source of polysaccharides, and might be regarded as a novel preventive and therapeutic product for the mitigation of insulin resistance, oxidative stress and liver dysfunction.
25157382	1	36	theme	High	166:169	arg1	intake					171:176	High intake	166:176	High intake of dietary fructose	166:196	High intake of dietary fructose exerts a number of adverse metabolic effects.
25157382	3	37	theme	main	609:612	arg1	monosaccharides					624:638	the main component monosaccharides	605:638	the main component monosaccharides	605:638	PEP was identified by HPLC as the heteropolysaccharides with d-glucose (62.8%, mol%), d-galactose (24.4%) and d-mannose (9.8%) being the main component monosaccharides.
25157382	5	38	theme	serum	924:928	arg1	glucose					930:936	the fasting serum glucose	912:936	the fasting serum glucose	912:936	The administration of PEP at 400 and 800 mg kg(-1) bw significantly reduced the fasting serum glucose and insulin concentrations and lipid deposition in HF-fed mice, and caused the reduction of liver lipid peroxidation and the elevation of the hepatic antioxidant system.
25157382	6	39	theme	liver	1197:1201	arg1	steatosis					1203:1211	the liver steatosis	1193:1211	the liver steatosis induced by a HF diet	1193:1232	The histopathology of the liver by conventional H&E and oil red O staining confirmed the liver steatosis induced by a HF diet and the hepatoprotective effect of PEP.
25157382	4	40	theme	p	825:825	arg1	sensitivity					812:822	impaired insulin sensitivity	795:822	impaired insulin sensitivity (p < 0.05)	795:833	Mice fed 20% fructose in drinking water for 6 weeks significantly displayed hyperglycemia, hyperinsulinemia, dyslipidemia and liver oxidative stress with impaired insulin sensitivity (p < 0.05).
25157382	4	40	theme	p	825:825	arg1	<					827:827	p < 0.05	825:832	p < 0.05	825:832	Mice fed 20% fructose in drinking water for 6 weeks significantly displayed hyperglycemia, hyperinsulinemia, dyslipidemia and liver oxidative stress with impaired insulin sensitivity (p < 0.05).
25157382	2	41	theme	eryngii	311:317	arg1	PEP					336:338	PEP	336:338	PEP	336:338	The present study investigates the preventive effects of Pleurotus eryngii polysaccharides (PEP), which showed powerful antioxidant activity in vitro, on insulin resistance and oxidative stress in mice fed a high-fructose diet.
25157382	2	41	theme	eryngii	311:317	arg1	polysaccharides					319:333	Pleurotus eryngii polysaccharides	301:333	Pleurotus eryngii polysaccharides (PEP)	301:339	The present study investigates the preventive effects of Pleurotus eryngii polysaccharides (PEP), which showed powerful antioxidant activity in vitro, on insulin resistance and oxidative stress in mice fed a high-fructose diet.
25157382	6	42	theme	O	1172:1172	arg1	staining					1174:1181	conventional H&E and oil red O staining	1143:1181	staining	1174:1181	The histopathology of the liver by conventional H&E and oil red O staining confirmed the liver steatosis induced by a HF diet and the hepatoprotective effect of PEP.
25157382	1	43	theme	dietary	181:187	arg1	fructose					189:196	dietary fructose	181:196	dietary fructose	181:196	High intake of dietary fructose exerts a number of adverse metabolic effects.
25157382	7	44	theme	polysaccharides	1344:1358	arg1	eryngii					1311:1317	Pleurotus eryngii	1301:1317	Pleurotus eryngii	1301:1317	These results suggest that Pleurotus eryngii is a potential source of polysaccharides, and might be regarded as a novel preventive and therapeutic product for the mitigation of insulin resistance, oxidative stress and liver dysfunction.
25157382	7	44	theme	polysaccharides	1344:1358	arg1	source					1334:1339	a potential source	1322:1339	a potential source of polysaccharides	1322:1358	These results suggest that Pleurotus eryngii is a potential source of polysaccharides, and might be regarded as a novel preventive and therapeutic product for the mitigation of insulin resistance, oxidative stress and liver dysfunction.
25157382	4	45	theme	impaired	795:802	arg1	sensitivity					812:822	impaired insulin sensitivity	795:822	impaired insulin sensitivity (p < 0.05)	795:833	Mice fed 20% fructose in drinking water for 6 weeks significantly displayed hyperglycemia, hyperinsulinemia, dyslipidemia and liver oxidative stress with impaired insulin sensitivity (p < 0.05).
25157382	4	45	theme	impaired	795:802	arg1	<					827:827	p < 0.05	825:832	p < 0.05	825:832	Mice fed 20% fructose in drinking water for 6 weeks significantly displayed hyperglycemia, hyperinsulinemia, dyslipidemia and liver oxidative stress with impaired insulin sensitivity (p < 0.05).
25157382	7	46	theme	potential	1324:1332	arg1	eryngii					1311:1317	Pleurotus eryngii	1301:1317	Pleurotus eryngii	1301:1317	These results suggest that Pleurotus eryngii is a potential source of polysaccharides, and might be regarded as a novel preventive and therapeutic product for the mitigation of insulin resistance, oxidative stress and liver dysfunction.
25157382	7	46	theme	potential	1324:1332	arg1	source					1334:1339	a potential source	1322:1339	a potential source of polysaccharides	1322:1358	These results suggest that Pleurotus eryngii is a potential source of polysaccharides, and might be regarded as a novel preventive and therapeutic product for the mitigation of insulin resistance, oxidative stress and liver dysfunction.
25157382	6	47	theme	oil	1164:1166	arg1	staining					1174:1181	conventional H&E and oil red O staining	1143:1181	staining	1174:1181	The histopathology of the liver by conventional H&E and oil red O staining confirmed the liver steatosis induced by a HF diet and the hepatoprotective effect of PEP.
25157382	4	48	with	hyperglycemia	717:729	arg1	sensitivity					812:822	impaired insulin sensitivity	795:822	impaired insulin sensitivity (p < 0.05)	795:833	Mice fed 20% fructose in drinking water for 6 weeks significantly displayed hyperglycemia, hyperinsulinemia, dyslipidemia and liver oxidative stress with impaired insulin sensitivity (p < 0.05).
25157382	4	48	with	hyperglycemia	717:729	arg1	<					827:827	p < 0.05	825:832	p < 0.05	825:832	Mice fed 20% fructose in drinking water for 6 weeks significantly displayed hyperglycemia, hyperinsulinemia, dyslipidemia and liver oxidative stress with impaired insulin sensitivity (p < 0.05).
25157382	3	49	theme	mol	551:553	arg1	%					548:548	62.8%	544:548	62.8%	544:548	PEP was identified by HPLC as the heteropolysaccharides with d-glucose (62.8%, mol%), d-galactose (24.4%) and d-mannose (9.8%) being the main component monosaccharides.
25157382	3	49	theme	mol	551:553	arg1	%					554:554	mol%	551:554	mol%	551:554	PEP was identified by HPLC as the heteropolysaccharides with d-glucose (62.8%, mol%), d-galactose (24.4%) and d-mannose (9.8%) being the main component monosaccharides.
25157382	5	50	from	concentrations	950:963	arg1	mice					996:999	HF-fed mice	989:999	HF-fed mice	989:999	The administration of PEP at 400 and 800 mg kg(-1) bw significantly reduced the fasting serum glucose and insulin concentrations and lipid deposition in HF-fed mice, and caused the reduction of liver lipid peroxidation and the elevation of the hepatic antioxidant system.
25157382	2	51	theme	present	248:254	arg1	study					256:260	The present study	244:260	The present study	244:260	The present study investigates the preventive effects of Pleurotus eryngii polysaccharides (PEP), which showed powerful antioxidant activity in vitro, on insulin resistance and oxidative stress in mice fed a high-fructose diet.
25157382	1	52	theme	adverse	217:223	arg1	effects					235:241	adverse metabolic effects	217:241	adverse metabolic effects	217:241	High intake of dietary fructose exerts a number of adverse metabolic effects.
25157382	0	53	from	effects	79:85	arg1	mice					160:163	mice	160:163	mice	160:163	Chemical composition of Pleurotus eryngii polysaccharides and their inhibitory effects on high-fructose diet-induced insulin resistance and oxidative stress in mice.
25157382	0	53	from	effects	79:85	arg1	resistance					125:134	high-fructose diet-induced insulin resistance	90:134	high-fructose diet-induced insulin resistance	90:134	Chemical composition of Pleurotus eryngii polysaccharides and their inhibitory effects on high-fructose diet-induced insulin resistance and oxidative stress in mice.
25157382	0	53	from	effects	79:85	arg1	stress					150:155	oxidative stress	140:155	oxidative stress	140:155	Chemical composition of Pleurotus eryngii polysaccharides and their inhibitory effects on high-fructose diet-induced insulin resistance and oxidative stress in mice.
25157382	6	54	theme	conventional	1143:1154	arg1	H&E					1156:1158	conventional H&E and oil red O staining	1143:1181	H&E	1156:1158	The histopathology of the liver by conventional H&E and oil red O staining confirmed the liver steatosis induced by a HF diet and the hepatoprotective effect of PEP.
25157382	6	55	theme	PEP	1269:1271	arg1	steatosis					1203:1211	the liver steatosis	1193:1211	the liver steatosis induced by a HF diet	1193:1232	The histopathology of the liver by conventional H&E and oil red O staining confirmed the liver steatosis induced by a HF diet and the hepatoprotective effect of PEP.
25157382	6	55	theme	PEP	1269:1271	arg1	effect					1259:1264	the hepatoprotective effect	1238:1264	the hepatoprotective effect of PEP	1238:1271	The histopathology of the liver by conventional H&E and oil red O staining confirmed the liver steatosis induced by a HF diet and the hepatoprotective effect of PEP.
25157382	5	56	theme	PEP	858:860	arg1	bw					887:888	PEP at 400 and 800 mg kg(-1) bw	858:888	PEP at 400 and 800 mg kg(-1) bw	858:888	The administration of PEP at 400 and 800 mg kg(-1) bw significantly reduced the fasting serum glucose and insulin concentrations and lipid deposition in HF-fed mice, and caused the reduction of liver lipid peroxidation and the elevation of the hepatic antioxidant system.
25157382	6	57	theme	liver	1134:1138	arg1	histopathology					1112:1125	The histopathology	1108:1125	The histopathology of the liver by conventional H&E and oil red O staining	1108:1181	The histopathology of the liver by conventional H&E and oil red O staining confirmed the liver steatosis induced by a HF diet and the hepatoprotective effect of PEP.
25157382	4	58	theme	%	652:652	arg1	fructose					654:661	20% fructose	650:661	20% fructose in drinking water for 6 weeks	650:691	Mice fed 20% fructose in drinking water for 6 weeks significantly displayed hyperglycemia, hyperinsulinemia, dyslipidemia and liver oxidative stress with impaired insulin sensitivity (p < 0.05).
25157382	5	59	theme	lipid	969:973	arg1	deposition					975:984	lipid deposition	969:984	lipid deposition in HF-fed mice	969:999	The administration of PEP at 400 and 800 mg kg(-1) bw significantly reduced the fasting serum glucose and insulin concentrations and lipid deposition in HF-fed mice, and caused the reduction of liver lipid peroxidation and the elevation of the hepatic antioxidant system.
25157382	2	60	theme	preventive	279:288	arg1	effects					290:296	the preventive effects	275:296	the preventive effects of Pleurotus eryngii polysaccharides (PEP), which showed powerful antioxidant activity in vitro, on insulin resistance and oxidative stress in mice fed a high-fructose diet	275:469	The present study investigates the preventive effects of Pleurotus eryngii polysaccharides (PEP), which showed powerful antioxidant activity in vitro, on insulin resistance and oxidative stress in mice fed a high-fructose diet.
25157382	2	61	from	effects	290:296	arg1	resistance					406:415	insulin resistance	398:415	insulin resistance	398:415	The present study investigates the preventive effects of Pleurotus eryngii polysaccharides (PEP), which showed powerful antioxidant activity in vitro, on insulin resistance and oxidative stress in mice fed a high-fructose diet.
25157382	2	61	from	effects	290:296	arg1	stress					431:436	oxidative stress	421:436	oxidative stress	421:436	The present study investigates the preventive effects of Pleurotus eryngii polysaccharides (PEP), which showed powerful antioxidant activity in vitro, on insulin resistance and oxidative stress in mice fed a high-fructose diet.
25157382	5	62	from	deposition	975:984	arg1	mice					996:999	HF-fed mice	989:999	HF-fed mice	989:999	The administration of PEP at 400 and 800 mg kg(-1) bw significantly reduced the fasting serum glucose and insulin concentrations and lipid deposition in HF-fed mice, and caused the reduction of liver lipid peroxidation and the elevation of the hepatic antioxidant system.
25157382	7	63	theme	oxidative	1471:1479	arg1	stress					1481:1486	oxidative stress	1471:1486	oxidative stress	1471:1486	These results suggest that Pleurotus eryngii is a potential source of polysaccharides, and might be regarded as a novel preventive and therapeutic product for the mitigation of insulin resistance, oxidative stress and liver dysfunction.
25157382	7	63	theme	oxidative	1471:1479	arg1	product					1421:1427	a novel preventive and therapeutic product	1386:1427	a novel preventive and therapeutic product for the mitigation of insulin resistance	1386:1468	These results suggest that Pleurotus eryngii is a potential source of polysaccharides, and might be regarded as a novel preventive and therapeutic product for the mitigation of insulin resistance, oxidative stress and liver dysfunction.
25157382	4	64	with	liver	767:771	arg1	sensitivity					812:822	impaired insulin sensitivity	795:822	impaired insulin sensitivity (p < 0.05)	795:833	Mice fed 20% fructose in drinking water for 6 weeks significantly displayed hyperglycemia, hyperinsulinemia, dyslipidemia and liver oxidative stress with impaired insulin sensitivity (p < 0.05).
25157382	4	64	with	liver	767:771	arg1	<					827:827	p < 0.05	825:832	p < 0.05	825:832	Mice fed 20% fructose in drinking water for 6 weeks significantly displayed hyperglycemia, hyperinsulinemia, dyslipidemia and liver oxidative stress with impaired insulin sensitivity (p < 0.05).
25157382	0	65	theme	high-fructose	90:102	arg1	resistance					125:134	high-fructose diet-induced insulin resistance	90:134	high-fructose diet-induced insulin resistance	90:134	Chemical composition of Pleurotus eryngii polysaccharides and their inhibitory effects on high-fructose diet-induced insulin resistance and oxidative stress in mice.
25157382	5	66	theme	lipid	1036:1040	arg1	peroxidation					1042:1053	liver lipid peroxidation	1030:1053	liver lipid peroxidation	1030:1053	The administration of PEP at 400 and 800 mg kg(-1) bw significantly reduced the fasting serum glucose and insulin concentrations and lipid deposition in HF-fed mice, and caused the reduction of liver lipid peroxidation and the elevation of the hepatic antioxidant system.
25157382	7	67	theme	resistance	1459:1468	arg1	mitigation					1437:1446	the mitigation	1433:1446	the mitigation of insulin resistance	1433:1468	These results suggest that Pleurotus eryngii is a potential source of polysaccharides, and might be regarded as a novel preventive and therapeutic product for the mitigation of insulin resistance, oxidative stress and liver dysfunction.
25157382	0	68	from	composition	9:19	arg1	mice					160:163	mice	160:163	mice	160:163	Chemical composition of Pleurotus eryngii polysaccharides and their inhibitory effects on high-fructose diet-induced insulin resistance and oxidative stress in mice.
25157382	0	68	from	composition	9:19	arg1	resistance					125:134	high-fructose diet-induced insulin resistance	90:134	high-fructose diet-induced insulin resistance	90:134	Chemical composition of Pleurotus eryngii polysaccharides and their inhibitory effects on high-fructose diet-induced insulin resistance and oxidative stress in mice.
25157382	0	68	from	composition	9:19	arg1	stress					150:155	oxidative stress	140:155	oxidative stress	140:155	Chemical composition of Pleurotus eryngii polysaccharides and their inhibitory effects on high-fructose diet-induced insulin resistance and oxidative stress in mice.
25157382	4	69	with	dyslipidemia	750:761	arg1	sensitivity					812:822	impaired insulin sensitivity	795:822	impaired insulin sensitivity (p < 0.05)	795:833	Mice fed 20% fructose in drinking water for 6 weeks significantly displayed hyperglycemia, hyperinsulinemia, dyslipidemia and liver oxidative stress with impaired insulin sensitivity (p < 0.05).
25157382	4	69	with	dyslipidemia	750:761	arg1	<					827:827	p < 0.05	825:832	p < 0.05	825:832	Mice fed 20% fructose in drinking water for 6 weeks significantly displayed hyperglycemia, hyperinsulinemia, dyslipidemia and liver oxidative stress with impaired insulin sensitivity (p < 0.05).
25157382	0	70	theme	insulin	117:123	arg1	resistance					125:134	high-fructose diet-induced insulin resistance	90:134	high-fructose diet-induced insulin resistance	90:134	Chemical composition of Pleurotus eryngii polysaccharides and their inhibitory effects on high-fructose diet-induced insulin resistance and oxidative stress in mice.
25157382	4	71	with	hyperinsulinemia	732:747	arg1	sensitivity					812:822	impaired insulin sensitivity	795:822	impaired insulin sensitivity (p < 0.05)	795:833	Mice fed 20% fructose in drinking water for 6 weeks significantly displayed hyperglycemia, hyperinsulinemia, dyslipidemia and liver oxidative stress with impaired insulin sensitivity (p < 0.05).
25157382	4	71	with	hyperinsulinemia	732:747	arg1	<					827:827	p < 0.05	825:832	p < 0.05	825:832	Mice fed 20% fructose in drinking water for 6 weeks significantly displayed hyperglycemia, hyperinsulinemia, dyslipidemia and liver oxidative stress with impaired insulin sensitivity (p < 0.05).
25157382	5	72	theme	bw	887:888	arg1	administration					840:853	The administration	836:853	The administration of PEP at 400 and 800 mg kg(-1) bw	836:888	The administration of PEP at 400 and 800 mg kg(-1) bw significantly reduced the fasting serum glucose and insulin concentrations and lipid deposition in HF-fed mice, and caused the reduction of liver lipid peroxidation and the elevation of the hepatic antioxidant system.
25157382	0	73	from	polysaccharides	42:56	arg1	mice					160:163	mice	160:163	mice	160:163	Chemical composition of Pleurotus eryngii polysaccharides and their inhibitory effects on high-fructose diet-induced insulin resistance and oxidative stress in mice.
25157382	0	73	from	polysaccharides	42:56	arg1	resistance					125:134	high-fructose diet-induced insulin resistance	90:134	high-fructose diet-induced insulin resistance	90:134	Chemical composition of Pleurotus eryngii polysaccharides and their inhibitory effects on high-fructose diet-induced insulin resistance and oxidative stress in mice.
25157382	0	73	from	polysaccharides	42:56	arg1	stress					150:155	oxidative stress	140:155	oxidative stress	140:155	Chemical composition of Pleurotus eryngii polysaccharides and their inhibitory effects on high-fructose diet-induced insulin resistance and oxidative stress in mice.
25157382	2	74	theme	insulin	398:404	arg1	resistance					406:415	insulin resistance	398:415	insulin resistance	398:415	The present study investigates the preventive effects of Pleurotus eryngii polysaccharides (PEP), which showed powerful antioxidant activity in vitro, on insulin resistance and oxidative stress in mice fed a high-fructose diet.
25157382	0	75	theme	Pleurotus	24:32	arg1	polysaccharides					42:56	Pleurotus eryngii polysaccharides	24:56	Pleurotus eryngii polysaccharides	24:56	Chemical composition of Pleurotus eryngii polysaccharides and their inhibitory effects on high-fructose diet-induced insulin resistance and oxidative stress in mice.
25157382	5	76	theme	antioxidant	1088:1098	arg1	system					1100:1105	the hepatic antioxidant system	1076:1105	the hepatic antioxidant system	1076:1105	The administration of PEP at 400 and 800 mg kg(-1) bw significantly reduced the fasting serum glucose and insulin concentrations and lipid deposition in HF-fed mice, and caused the reduction of liver lipid peroxidation and the elevation of the hepatic antioxidant system.
25157382	0	77	theme	polysaccharides	42:56	arg1	composition					9:19	Chemical composition	0:19	Chemical composition of Pleurotus eryngii polysaccharides and their inhibitory effects on high-fructose diet-induced insulin resistance and oxidative stress in mice.	0:164	Chemical composition of Pleurotus eryngii polysaccharides and their inhibitory effects on high-fructose diet-induced insulin resistance and oxidative stress in mice.
25157382	5	78	theme	glucose	930:936	arg1	concentrations					950:963	the fasting serum glucose and insulin concentrations	912:963	the fasting serum glucose and insulin concentrations	912:963	The administration of PEP at 400 and 800 mg kg(-1) bw significantly reduced the fasting serum glucose and insulin concentrations and lipid deposition in HF-fed mice, and caused the reduction of liver lipid peroxidation and the elevation of the hepatic antioxidant system.
25157382	5	79	theme	insulin	942:948	arg1	concentrations					950:963	the fasting serum glucose and insulin concentrations	912:963	the fasting serum glucose and insulin concentrations	912:963	The administration of PEP at 400 and 800 mg kg(-1) bw significantly reduced the fasting serum glucose and insulin concentrations and lipid deposition in HF-fed mice, and caused the reduction of liver lipid peroxidation and the elevation of the hepatic antioxidant system.
25157382	3	80	theme	component	614:622	arg1	monosaccharides					624:638	the main component monosaccharides	605:638	the main component monosaccharides	605:638	PEP was identified by HPLC as the heteropolysaccharides with d-glucose (62.8%, mol%), d-galactose (24.4%) and d-mannose (9.8%) being the main component monosaccharides.
25157382	5	81	from	kg	880:881	arg1	PEP					858:860	PEP	858:860	PEP	858:860	The administration of PEP at 400 and 800 mg kg(-1) bw significantly reduced the fasting serum glucose and insulin concentrations and lipid deposition in HF-fed mice, and caused the reduction of liver lipid peroxidation and the elevation of the hepatic antioxidant system.
25157382	7	82	theme	therapeutic	1409:1419	arg1	eryngii					1311:1317	Pleurotus eryngii	1301:1317	Pleurotus eryngii	1301:1317	These results suggest that Pleurotus eryngii is a potential source of polysaccharides, and might be regarded as a novel preventive and therapeutic product for the mitigation of insulin resistance, oxidative stress and liver dysfunction.
25157382	7	82	theme	therapeutic	1409:1419	arg1	stress					1481:1486	oxidative stress	1471:1486	oxidative stress	1471:1486	These results suggest that Pleurotus eryngii is a potential source of polysaccharides, and might be regarded as a novel preventive and therapeutic product for the mitigation of insulin resistance, oxidative stress and liver dysfunction.
25157382	7	82	theme	therapeutic	1409:1419	arg1	dysfunction					1498:1508	liver dysfunction	1492:1508	liver dysfunction	1492:1508	These results suggest that Pleurotus eryngii is a potential source of polysaccharides, and might be regarded as a novel preventive and therapeutic product for the mitigation of insulin resistance, oxidative stress and liver dysfunction.
25157382	7	82	theme	therapeutic	1409:1419	arg1	product					1421:1427	a novel preventive and therapeutic product	1386:1427	a novel preventive and therapeutic product for the mitigation of insulin resistance	1386:1468	These results suggest that Pleurotus eryngii is a potential source of polysaccharides, and might be regarded as a novel preventive and therapeutic product for the mitigation of insulin resistance, oxidative stress and liver dysfunction.
25157382	0	83	dep	Pleurotus	24:32	arg1	eryngii					34:40	eryngii	34:40	eryngii	34:40	Chemical composition of Pleurotus eryngii polysaccharides and their inhibitory effects on high-fructose diet-induced insulin resistance and oxidative stress in mice.
25157382	0	84	from	mice	160:163	arg1	composition					9:19	Chemical composition	0:19	Chemical composition of Pleurotus eryngii polysaccharides and their inhibitory effects on high-fructose diet-induced insulin resistance and oxidative stress in mice.	0:164	Chemical composition of Pleurotus eryngii polysaccharides and their inhibitory effects on high-fructose diet-induced insulin resistance and oxidative stress in mice.
25157382	4	85	from	fructose	654:661	arg1	water					675:679	drinking water	666:679	drinking water	666:679	Mice fed 20% fructose in drinking water for 6 weeks significantly displayed hyperglycemia, hyperinsulinemia, dyslipidemia and liver oxidative stress with impaired insulin sensitivity (p < 0.05).
25157382	7	86	theme	preventive	1394:1403	arg1	eryngii					1311:1317	Pleurotus eryngii	1301:1317	Pleurotus eryngii	1301:1317	These results suggest that Pleurotus eryngii is a potential source of polysaccharides, and might be regarded as a novel preventive and therapeutic product for the mitigation of insulin resistance, oxidative stress and liver dysfunction.
25157382	7	86	theme	preventive	1394:1403	arg1	stress					1481:1486	oxidative stress	1471:1486	oxidative stress	1471:1486	These results suggest that Pleurotus eryngii is a potential source of polysaccharides, and might be regarded as a novel preventive and therapeutic product for the mitigation of insulin resistance, oxidative stress and liver dysfunction.
25157382	7	86	theme	preventive	1394:1403	arg1	dysfunction					1498:1508	liver dysfunction	1492:1508	liver dysfunction	1492:1508	These results suggest that Pleurotus eryngii is a potential source of polysaccharides, and might be regarded as a novel preventive and therapeutic product for the mitigation of insulin resistance, oxidative stress and liver dysfunction.
25157382	7	86	theme	preventive	1394:1403	arg1	product					1421:1427	a novel preventive and therapeutic product	1386:1427	a novel preventive and therapeutic product for the mitigation of insulin resistance	1386:1468	These results suggest that Pleurotus eryngii is a potential source of polysaccharides, and might be regarded as a novel preventive and therapeutic product for the mitigation of insulin resistance, oxidative stress and liver dysfunction.
25157382	5	87	theme	fasting	916:922	arg1	glucose					930:936	the fasting serum glucose	912:936	the fasting serum glucose	912:936	The administration of PEP at 400 and 800 mg kg(-1) bw significantly reduced the fasting serum glucose and insulin concentrations and lipid deposition in HF-fed mice, and caused the reduction of liver lipid peroxidation and the elevation of the hepatic antioxidant system.
25157382	0	88	from	stress	150:155	arg1	composition					9:19	Chemical composition	0:19	Chemical composition of Pleurotus eryngii polysaccharides and their inhibitory effects on high-fructose diet-induced insulin resistance and oxidative stress in mice.	0:164	Chemical composition of Pleurotus eryngii polysaccharides and their inhibitory effects on high-fructose diet-induced insulin resistance and oxidative stress in mice.
28958543	9	0	theme	frequency	886:894	arg1	dependence					896:905	frequency dependence	886:905	frequency dependence	886:905	The effects of HHP on some rheological properties such as frequency dependence were different between these two types of starches.
28958543	10	1	theme	starch	1061:1066	arg1	structures					1047:1056	granular and chemical structures	1025:1056	granular and chemical structures of starch	1025:1066	The differences could be attributed to the different composition, granular and chemical structures of starch, and the presence of granule remnants.
28958543	10	1	theme	starch	1061:1066	arg1	composition					1012:1022	the different composition	998:1022	the different composition	998:1022	The differences could be attributed to the different composition, granular and chemical structures of starch, and the presence of granule remnants.
28958543	10	1	theme	starch	1061:1066	arg1	presence					1077:1084	the presence	1073:1084	the presence of granule remnants	1073:1104	The differences could be attributed to the different composition, granular and chemical structures of starch, and the presence of granule remnants.
28958543	1	2	theme	low	141:143	arg1	temperatures					160:171	relatively low gelatinization temperatures	130:171	relatively low gelatinization temperatures	130:171	Quinoa starch has small granules with relatively low gelatinization temperatures and amylose content.
28958543	7	3	theme	amylopectin	689:699	arg1	recrystallization					701:717	amylopectin recrystallization and gel textural properties	689:745	recrystallization	701:717	HHP had little effects on amylopectin recrystallization and gel textural properties of starch.
28958543	0	4	theme	quinoa	65:70	arg1	starches					82:89	quinoa and maize starches	65:89	quinoa and maize starches	65:89	Effect of high pressure on rheological and thermal properties of quinoa and maize starches.
28958543	1	5	contain	has	106:108	arg1	starch					99:104	Quinoa starch	92:104	Quinoa starch	92:104	Quinoa starch has small granules with relatively low gelatinization temperatures and amylose content.
28958543	1	5	contain	has	106:108	arg2	granules					116:123	small granules	110:123	small granules	110:123	Quinoa starch has small granules with relatively low gelatinization temperatures and amylose content.
28958543	1	6	theme	gelatinization	145:158	arg1	temperatures					160:171	relatively low gelatinization temperatures	130:171	relatively low gelatinization temperatures	130:171	Quinoa starch has small granules with relatively low gelatinization temperatures and amylose content.
28958543	0	7	theme	starches	82:89	arg1	properties					51:60	rheological and thermal properties	27:60	rheological and thermal properties of quinoa and maize starches	27:89	Effect of high pressure on rheological and thermal properties of quinoa and maize starches.
28958543	0	8	from	Effect	0:5	arg1	properties					51:60	rheological and thermal properties	27:60	rheological and thermal properties of quinoa and maize starches	27:89	Effect of high pressure on rheological and thermal properties of quinoa and maize starches.
28958543	0	9	theme	maize	76:80	arg1	starches					82:89	quinoa and maize starches	65:89	quinoa and maize starches	65:89	Effect of high pressure on rheological and thermal properties of quinoa and maize starches.
28958543	10	10	theme	chemical	1038:1045	arg1	structures					1047:1056	granular and chemical structures	1025:1056	granular and chemical structures of starch	1025:1066	The differences could be attributed to the different composition, granular and chemical structures of starch, and the presence of granule remnants.
28958543	9	11	from	effects	832:838	arg1	dependence					896:905	frequency dependence	886:905	frequency dependence	886:905	The effects of HHP on some rheological properties such as frequency dependence were different between these two types of starches.
28958543	9	11	from	effects	832:838	arg1	properties					867:876	some rheological properties	850:876	some rheological properties such as frequency dependence	850:905	The effects of HHP on some rheological properties such as frequency dependence were different between these two types of starches.
28958543	7	12	theme	gel	723:725	arg1	properties					736:745	amylopectin recrystallization and gel textural properties	689:745	properties	736:745	HHP had little effects on amylopectin recrystallization and gel textural properties of starch.
28958543	8	13	theme	quinoa	767:772	arg1	starch					774:779	quinoa starch	767:779	quinoa starch	767:779	Overall, quinoa starch was more susceptible to HHP than maize starch.
28958543	2	14	theme	food	257:260	arg1	processing					262:271	food processing	257:271	food processing	257:271	High hydrostatic pressure (HHP) is a non-thermal technique for food processing.
28958543	9	15	theme	starches	949:956	arg1	types					940:944	these two types	930:944	these two types of starches	930:956	The effects of HHP on some rheological properties such as frequency dependence were different between these two types of starches.
28958543	3	16	theme	normal	399:404	arg1	starch					412:417	a normal maize starch	397:417	a normal maize starch	397:417	In this study, effects of HHP up to 600MPa on physical properties of quinoa starch were studied and compared with those of a normal maize starch.
28958543	3	17	theme	starch	350:355	arg1	properties					329:338	physical properties	320:338	physical properties of quinoa starch	320:355	In this study, effects of HHP up to 600MPa on physical properties of quinoa starch were studied and compared with those of a normal maize starch.
28958543	3	18	theme	HHP	300:302	arg1	up					304:305	HHP up	300:305	HHP up to 600MPa	300:315	In this study, effects of HHP up to 600MPa on physical properties of quinoa starch were studied and compared with those of a normal maize starch.
28958543	1	19	theme	amylose	177:183	arg1	content					185:191	amylose content	177:191	amylose content	177:191	Quinoa starch has small granules with relatively low gelatinization temperatures and amylose content.
28958543	6	20	theme	rheological	566:576	arg1	analysis					578:585	Dynamic rheological analysis	558:585	Dynamic rheological analysis	558:585	Dynamic rheological analysis showed that HHP improved the gel stability of both starches during cooling.
28958543	10	21	theme	remnants	1097:1104	arg1	structures					1047:1056	granular and chemical structures	1025:1056	granular and chemical structures of starch	1025:1066	The differences could be attributed to the different composition, granular and chemical structures of starch, and the presence of granule remnants.
28958543	10	21	theme	remnants	1097:1104	arg1	composition					1012:1022	the different composition	998:1022	the different composition	998:1022	The differences could be attributed to the different composition, granular and chemical structures of starch, and the presence of granule remnants.
28958543	10	21	theme	remnants	1097:1104	arg1	presence					1077:1084	the presence	1073:1084	the presence of granule remnants	1073:1104	The differences could be attributed to the different composition, granular and chemical structures of starch, and the presence of granule remnants.
28958543	5	22	theme	thermal	540:546	arg1	analysis					548:555	thermal analysis	540:555	thermal analysis	540:555	The pressure of 600MPa completely gelatinized quinoa starch as revealed by thermal analysis.
28958543	0	23	theme	pressure	15:22	arg1	Effect					0:5	Effect	0:5	Effect of high pressure on rheological and thermal properties of quinoa and maize starches.	0:90	Effect of high pressure on rheological and thermal properties of quinoa and maize starches.
28958543	10	24	theme	granule	1089:1095	arg1	remnants					1097:1104	granule remnants	1089:1104	granule remnants	1089:1104	The differences could be attributed to the different composition, granular and chemical structures of starch, and the presence of granule remnants.
28958543	3	25	theme	quinoa	343:348	arg1	starch					350:355	quinoa starch	343:355	quinoa starch	343:355	In this study, effects of HHP up to 600MPa on physical properties of quinoa starch were studied and compared with those of a normal maize starch.
28958543	1	26	theme	Quinoa	92:97	arg1	starch					99:104	Quinoa starch	92:104	Quinoa starch	92:104	Quinoa starch has small granules with relatively low gelatinization temperatures and amylose content.
28958543	6	27	theme	starches	638:645	arg1	stability					620:628	the gel stability	612:628	the gel stability of both starches	612:645	Dynamic rheological analysis showed that HHP improved the gel stability of both starches during cooling.
28958543	0	28	theme	rheological	27:37	arg1	properties					51:60	rheological and thermal properties	27:60	rheological and thermal properties of quinoa and maize starches	27:89	Effect of high pressure on rheological and thermal properties of quinoa and maize starches.
28958543	7	29	theme	little	671:676	arg1	effects					678:684	little effects	671:684	little effects	671:684	HHP had little effects on amylopectin recrystallization and gel textural properties of starch.
28958543	3	30	theme	maize	406:410	arg1	starch					412:417	a normal maize starch	397:417	a normal maize starch	397:417	In this study, effects of HHP up to 600MPa on physical properties of quinoa starch were studied and compared with those of a normal maize starch.
28958543	9	31	theme	HHP	843:845	arg1	different					912:920	different	912:920	different	912:920	The effects of HHP on some rheological properties such as frequency dependence were different between these two types of starches.
28958543	9	31	theme	HHP	843:845	arg1	effects					832:838	The effects	828:838	The effects of HHP on some rheological properties such as frequency dependence	828:905	The effects of HHP on some rheological properties such as frequency dependence were different between these two types of starches.
28958543	6	32	theme	Dynamic	558:564	arg1	analysis					578:585	Dynamic rheological analysis	558:585	Dynamic rheological analysis	558:585	Dynamic rheological analysis showed that HHP improved the gel stability of both starches during cooling.
28958543	7	33	contain	had	667:669	arg1	HHP					663:665	HHP	663:665	HHP	663:665	HHP had little effects on amylopectin recrystallization and gel textural properties of starch.
28958543	7	33	contain	had	667:669	arg2	effects					678:684	little effects	671:684	little effects	671:684	HHP had little effects on amylopectin recrystallization and gel textural properties of starch.
28958543	0	34	theme	thermal	43:49	arg1	properties					51:60	rheological and thermal properties	27:60	rheological and thermal properties of quinoa and maize starches	27:89	Effect of high pressure on rheological and thermal properties of quinoa and maize starches.
28958543	3	35	theme	physical	320:327	arg1	properties					329:338	physical properties	320:338	physical properties of quinoa starch	320:355	In this study, effects of HHP up to 600MPa on physical properties of quinoa starch were studied and compared with those of a normal maize starch.
28958543	5	36	theme	quinoa	511:516	arg1	starch					518:523	quinoa starch	511:523	quinoa starch	511:523	The pressure of 600MPa completely gelatinized quinoa starch as revealed by thermal analysis.
28958543	10	37	theme	granular	1025:1032	arg1	structures					1047:1056	granular and chemical structures	1025:1056	granular and chemical structures of starch	1025:1066	The differences could be attributed to the different composition, granular and chemical structures of starch, and the presence of granule remnants.
28958543	7	38	theme	starch	750:755	arg1	recrystallization					701:717	amylopectin recrystallization and gel textural properties	689:745	recrystallization	701:717	HHP had little effects on amylopectin recrystallization and gel textural properties of starch.
28958543	7	38	theme	starch	750:755	arg1	properties					736:745	amylopectin recrystallization and gel textural properties	689:745	properties	736:745	HHP had little effects on amylopectin recrystallization and gel textural properties of starch.
28958543	9	39	theme	rheological	855:865	arg1	dependence					896:905	frequency dependence	886:905	frequency dependence	886:905	The effects of HHP on some rheological properties such as frequency dependence were different between these two types of starches.
28958543	9	39	theme	rheological	855:865	arg1	properties					867:876	some rheological properties	850:876	some rheological properties such as frequency dependence	850:905	The effects of HHP on some rheological properties such as frequency dependence were different between these two types of starches.
28958543	2	40	theme	hydrostatic	199:209	arg1	technique					243:251	a non-thermal technique	229:251	a non-thermal technique for food processing	229:271	High hydrostatic pressure (HHP) is a non-thermal technique for food processing.
28958543	2	40	theme	hydrostatic	199:209	arg1	HHP					221:223	HHP	221:223	HHP	221:223	High hydrostatic pressure (HHP) is a non-thermal technique for food processing.
28958543	2	40	theme	hydrostatic	199:209	arg1	pressure					211:218	High hydrostatic pressure	194:218	High hydrostatic pressure (HHP)	194:224	High hydrostatic pressure (HHP) is a non-thermal technique for food processing.
28958543	1	41	theme	small	110:114	arg1	granules					116:123	small granules	110:123	small granules	110:123	Quinoa starch has small granules with relatively low gelatinization temperatures and amylose content.
28958543	7	42	theme	textural	727:734	arg1	properties					736:745	amylopectin recrystallization and gel textural properties	689:745	properties	736:745	HHP had little effects on amylopectin recrystallization and gel textural properties of starch.
28958543	2	43	theme	High	194:197	arg1	technique					243:251	a non-thermal technique	229:251	a non-thermal technique for food processing	229:271	High hydrostatic pressure (HHP) is a non-thermal technique for food processing.
28958543	2	43	theme	High	194:197	arg1	HHP					221:223	HHP	221:223	HHP	221:223	High hydrostatic pressure (HHP) is a non-thermal technique for food processing.
28958543	2	43	theme	High	194:197	arg1	pressure					211:218	High hydrostatic pressure	194:218	High hydrostatic pressure (HHP)	194:224	High hydrostatic pressure (HHP) is a non-thermal technique for food processing.
28958543	6	44	theme	gel	616:618	arg1	stability					620:628	the gel stability	612:628	the gel stability of both starches	612:645	Dynamic rheological analysis showed that HHP improved the gel stability of both starches during cooling.
28958543	3	45	theme	up	304:305	arg1	effects					289:295	effects	289:295	effects of HHP up to 600MPa on physical properties of quinoa starch	289:355	In this study, effects of HHP up to 600MPa on physical properties of quinoa starch were studied and compared with those of a normal maize starch.
28958543	10	46	theme	different	1002:1010	arg1	composition					1012:1022	the different composition	998:1022	the different composition	998:1022	The differences could be attributed to the different composition, granular and chemical structures of starch, and the presence of granule remnants.
28958543	2	47	theme	non-thermal	231:241	arg1	technique					243:251	a non-thermal technique	229:251	a non-thermal technique for food processing	229:271	High hydrostatic pressure (HHP) is a non-thermal technique for food processing.
28958543	2	47	theme	non-thermal	231:241	arg1	pressure					211:218	High hydrostatic pressure	194:218	High hydrostatic pressure (HHP)	194:224	High hydrostatic pressure (HHP) is a non-thermal technique for food processing.
28958543	0	48	theme	high	10:13	arg1	pressure					15:22	high pressure	10:22	high pressure	10:22	Effect of high pressure on rheological and thermal properties of quinoa and maize starches.
28958543	5	49	theme	600MPa	481:486	arg1	pressure					469:476	The pressure	465:476	The pressure of 600MPa	465:486	The pressure of 600MPa completely gelatinized quinoa starch as revealed by thermal analysis.
28958543	8	50	theme	maize	814:818	arg1	starch					820:825	maize starch	814:825	maize starch	814:825	Overall, quinoa starch was more susceptible to HHP than maize starch.
28958543	3	51	from	effects	289:295	arg1	properties					329:338	physical properties	320:338	physical properties of quinoa starch	320:355	In this study, effects of HHP up to 600MPa on physical properties of quinoa starch were studied and compared with those of a normal maize starch.
27845025	4	0	theme	whey	799:802	arg1	proteins					789:796	proteins	789:796	proteins (whey protein and skim milk powder)	789:832	The addition of the carbohydrates (inulin and maltodextrin) and proteins (whey protein and skim milk powder) also influenced the colour of the low-fat cupuassu goat milk yogurt (P < 0·05).
27845025	4	0	theme	whey	799:802	arg1	protein					804:810	whey protein	799:810	whey protein	799:810	The addition of the carbohydrates (inulin and maltodextrin) and proteins (whey protein and skim milk powder) also influenced the colour of the low-fat cupuassu goat milk yogurt (P < 0·05).
27845025	4	1	theme	low-fat	868:874	arg1	yogurt					895:900	the low-fat cupuassu goat milk yogurt	864:900	the low-fat cupuassu goat milk yogurt (P < 0·05)	864:911	The addition of the carbohydrates (inulin and maltodextrin) and proteins (whey protein and skim milk powder) also influenced the colour of the low-fat cupuassu goat milk yogurt (P < 0·05).
27845025	4	1	theme	low-fat	868:874	arg1	0·05					907:910	P < 0·05	903:910	P < 0·05	903:910	The addition of the carbohydrates (inulin and maltodextrin) and proteins (whey protein and skim milk powder) also influenced the colour of the low-fat cupuassu goat milk yogurt (P < 0·05).
27845025	1	2	theme	Research	138:145	arg1	Communication					147:159	this Research Communication	133:159	this Research Communication	133:159	The aim of this Research Communication was to investigate the changes in physicochemical, colour, apparent viscosity and texture properties in low-fat goat milk yogurts prepared with cupuassu pulp by the addition of inulin (SI), maltodextrin (SM), whey protein (SW) and skim milk powder (SP).
27845025	5	3	theme	<	984:984	arg1	0·05					986:989	P < 0·05	982:989	P < 0·05	982:989	All fat replacer treatments (SI, SM, SW and SP) presented a higher (P < 0·05) apparent viscosity than W and S yogurts.
27845025	3	4	theme	fat	659:661	arg1	replacers					663:671	the fat replacers	655:671	the fat replacers	655:671	In comparison to yogurts from whole (W) or skimmed milk (S), all of the fat replacers improved the physicochemical properties (P < 0·05).
27845025	6	5	theme	powder	1073:1078	arg1	addition					1051:1058	only the addition	1042:1058	only the addition of skim milk powder	1042:1078	However, only the addition of skim milk powder increased the texture parameters (firmness and consistency) (P < 0·05).
27845025	7	6	theme	skim	1179:1182	arg1	milk					1184:1187	skim milk	1179:1187	skim milk powder	1179:1194	These results suggest that skim milk powder can be used to improve the texture properties of low-fat cupuassu goat milk.
27845025	1	7	theme	Communication	147:159	arg1	aim					126:128	The aim	122:128	The aim of this Research Communication	122:159	The aim of this Research Communication was to investigate the changes in physicochemical, colour, apparent viscosity and texture properties in low-fat goat milk yogurts prepared with cupuassu pulp by the addition of inulin (SI), maltodextrin (SM), whey protein (SW) and skim milk powder (SP).
27845025	1	8	dep	protein	375:381	arg1	powder					402:407	powder	402:407	powder	402:407	The aim of this Research Communication was to investigate the changes in physicochemical, colour, apparent viscosity and texture properties in low-fat goat milk yogurts prepared with cupuassu pulp by the addition of inulin (SI), maltodextrin (SM), whey protein (SW) and skim milk powder (SP).
27845025	0	9	theme	cupuassu	94:101	arg1	milk					108:111	low-fat cupuassu goat milk	86:111	low-fat cupuassu goat milk	86:111	Effect of different fat replacers on the physicochemical and instrumental analysis of low-fat cupuassu goat milk yogurts.
27845025	0	10	from	Effect	0:5	arg1	analysis					74:81	the physicochemical and instrumental analysis	37:81	the physicochemical and instrumental analysis of low-fat cupuassu goat milk	37:111	Effect of different fat replacers on the physicochemical and instrumental analysis of low-fat cupuassu goat milk yogurts.
27845025	2	11	theme	1st	497:499	arg1	day					501:503	the 1st day	493:503	the 1st day of storage	493:514	Three batches of each cupuassu goat milk yogurt were prepared and analysed on the 1st day of storage by pH, proximate composition, colour, apparent viscosity, and texture.
27845025	3	12	theme	P	714:714	arg1	properties					702:711	the physicochemical properties	682:711	the physicochemical properties (P < 0·05)	682:722	In comparison to yogurts from whole (W) or skimmed milk (S), all of the fat replacers improved the physicochemical properties (P < 0·05).
27845025	3	12	theme	P	714:714	arg1	0·05					718:721	P < 0·05	714:721	P < 0·05	714:721	In comparison to yogurts from whole (W) or skimmed milk (S), all of the fat replacers improved the physicochemical properties (P < 0·05).
27845025	6	13	theme	skim	1063:1066	arg1	milk					1068:1071	skim milk	1063:1071	skim milk powder	1063:1078	However, only the addition of skim milk powder increased the texture parameters (firmness and consistency) (P < 0·05).
27845025	0	14	theme	low-fat	86:92	arg1	milk					108:111	low-fat cupuassu goat milk	86:111	low-fat cupuassu goat milk	86:111	Effect of different fat replacers on the physicochemical and instrumental analysis of low-fat cupuassu goat milk yogurts.
27845025	1	15	theme	inulin	338:343	arg1	addition					326:333	the addition	322:333	the addition of inulin (SI), maltodextrin (SM), whey protein (SW) and skim milk powder (SP)	322:412	The aim of this Research Communication was to investigate the changes in physicochemical, colour, apparent viscosity and texture properties in low-fat goat milk yogurts prepared with cupuassu pulp by the addition of inulin (SI), maltodextrin (SM), whey protein (SW) and skim milk powder (SP).
27845025	4	16	dep	protein	804:810	arg1	powder					826:831	powder	826:831	powder	826:831	The addition of the carbohydrates (inulin and maltodextrin) and proteins (whey protein and skim milk powder) also influenced the colour of the low-fat cupuassu goat milk yogurt (P < 0·05).
27845025	6	17	dep	parameters	1102:1111	arg1	firmness					1114:1121	firmness	1114:1121	firmness	1114:1121	However, only the addition of skim milk powder increased the texture parameters (firmness and consistency) (P < 0·05).
27845025	6	17	dep	parameters	1102:1111	arg1	parameters					1102:1111	the texture parameters	1090:1111	the texture parameters (firmness and consistency) (P < 0·05)	1090:1149	However, only the addition of skim milk powder increased the texture parameters (firmness and consistency) (P < 0·05).
27845025	6	17	dep	parameters	1102:1111	arg1	consistency					1127:1137	consistency	1127:1137	consistency	1127:1137	However, only the addition of skim milk powder increased the texture parameters (firmness and consistency) (P < 0·05).
27845025	7	18	theme	texture	1223:1229	arg1	properties					1231:1240	the texture properties	1219:1240	the texture properties of low-fat cupuassu goat milk	1219:1270	These results suggest that skim milk powder can be used to improve the texture properties of low-fat cupuassu goat milk.
27845025	0	19	theme	milk	108:111	arg1	analysis					74:81	the physicochemical and instrumental analysis	37:81	the physicochemical and instrumental analysis of low-fat cupuassu goat milk	37:111	Effect of different fat replacers on the physicochemical and instrumental analysis of low-fat cupuassu goat milk yogurts.
27845025	8	20	theme	dairy	1364:1368	arg1	industry					1370:1377	the goat dairy industry	1355:1377	the goat dairy industry	1355:1377	Furthermore, inulin, maltodextrin, and whey protein can potentially be applied in the goat dairy industry to increase the viscosity of yogurts.
27845025	3	21	from	whole	617:621	arg1	yogurts					604:610	yogurts	604:610	yogurts from whole (W) or skimmed milk (S)	604:645	In comparison to yogurts from whole (W) or skimmed milk (S), all of the fat replacers improved the physicochemical properties (P < 0·05).
27845025	2	22	theme	proximate	523:531	arg1	composition					533:543	proximate composition	523:543	proximate composition	523:543	Three batches of each cupuassu goat milk yogurt were prepared and analysed on the 1st day of storage by pH, proximate composition, colour, apparent viscosity, and texture.
27845025	8	23	theme	goat	1359:1362	arg1	industry					1370:1377	the goat dairy industry	1355:1377	the goat dairy industry	1355:1377	Furthermore, inulin, maltodextrin, and whey protein can potentially be applied in the goat dairy industry to increase the viscosity of yogurts.
27845025	0	24	theme	goat	103:106	arg1	milk					108:111	low-fat cupuassu goat milk	86:111	low-fat cupuassu goat milk	86:111	Effect of different fat replacers on the physicochemical and instrumental analysis of low-fat cupuassu goat milk yogurts.
27845025	7	25	theme	cupuassu	1253:1260	arg1	milk					1267:1270	low-fat cupuassu goat milk	1245:1270	low-fat cupuassu goat milk	1245:1270	These results suggest that skim milk powder can be used to improve the texture properties of low-fat cupuassu goat milk.
27845025	1	26	theme	skim	392:395	arg1	milk					397:400	skim milk	392:400	skim milk	392:400	The aim of this Research Communication was to investigate the changes in physicochemical, colour, apparent viscosity and texture properties in low-fat goat milk yogurts prepared with cupuassu pulp by the addition of inulin (SI), maltodextrin (SM), whey protein (SW) and skim milk powder (SP).
27845025	1	26	theme	skim	392:395	arg1	inulin					338:343	inulin	338:343	inulin (SI)	338:348	The aim of this Research Communication was to investigate the changes in physicochemical, colour, apparent viscosity and texture properties in low-fat goat milk yogurts prepared with cupuassu pulp by the addition of inulin (SI), maltodextrin (SM), whey protein (SW) and skim milk powder (SP).
27845025	5	27	theme	apparent	992:999	arg1	viscosity					1001:1009	a higher (P < 0·05) apparent viscosity	972:1009	a higher (P < 0·05) apparent viscosity than W and S yogurts	972:1030	All fat replacer treatments (SI, SM, SW and SP) presented a higher (P < 0·05) apparent viscosity than W and S yogurts.
27845025	4	28	theme	proteins	789:796	arg1	addition					729:736	The addition	725:736	The addition of the carbohydrates (inulin and maltodextrin) and proteins (whey protein and skim milk powder)	725:832	The addition of the carbohydrates (inulin and maltodextrin) and proteins (whey protein and skim milk powder) also influenced the colour of the low-fat cupuassu goat milk yogurt (P < 0·05).
27845025	5	29	theme	W	1016:1016	arg1	yogurts					1024:1030	W and S yogurts	1016:1030	W and S yogurts	1016:1030	All fat replacer treatments (SI, SM, SW and SP) presented a higher (P < 0·05) apparent viscosity than W and S yogurts.
27845025	7	30	theme	low-fat	1245:1251	arg1	milk					1267:1270	low-fat cupuassu goat milk	1245:1270	low-fat cupuassu goat milk	1245:1270	These results suggest that skim milk powder can be used to improve the texture properties of low-fat cupuassu goat milk.
27845025	1	31	theme	low-fat	265:271	arg1	yogurts					283:289	low-fat goat milk yogurts	265:289	low-fat goat milk yogurts prepared with cupuassu pulp by the addition of inulin (SI), maltodextrin (SM), whey protein (SW) and skim milk powder (SP)	265:412	The aim of this Research Communication was to investigate the changes in physicochemical, colour, apparent viscosity and texture properties in low-fat goat milk yogurts prepared with cupuassu pulp by the addition of inulin (SI), maltodextrin (SM), whey protein (SW) and skim milk powder (SP).
27845025	2	32	theme	storage	508:514	arg1	day					501:503	the 1st day	493:503	the 1st day of storage	493:514	Three batches of each cupuassu goat milk yogurt were prepared and analysed on the 1st day of storage by pH, proximate composition, colour, apparent viscosity, and texture.
27845025	2	33	theme	apparent	554:561	arg1	viscosity					563:571	apparent viscosity	554:571	apparent viscosity	554:571	Three batches of each cupuassu goat milk yogurt were prepared and analysed on the 1st day of storage by pH, proximate composition, colour, apparent viscosity, and texture.
27845025	0	34	theme	fat	20:22	arg1	replacers					24:32	different fat replacers	10:32	different fat replacers	10:32	Effect of different fat replacers on the physicochemical and instrumental analysis of low-fat cupuassu goat milk yogurts.
27845025	1	35	theme	goat	273:276	arg1	yogurts					283:289	low-fat goat milk yogurts	265:289	low-fat goat milk yogurts prepared with cupuassu pulp by the addition of inulin (SI), maltodextrin (SM), whey protein (SW) and skim milk powder (SP)	265:412	The aim of this Research Communication was to investigate the changes in physicochemical, colour, apparent viscosity and texture properties in low-fat goat milk yogurts prepared with cupuassu pulp by the addition of inulin (SI), maltodextrin (SM), whey protein (SW) and skim milk powder (SP).
27845025	5	36	theme	fat	918:920	arg1	treatments					931:940	All fat replacer treatments	914:940	All fat replacer treatments (SI, SM, SW and SP)	914:960	All fat replacer treatments (SI, SM, SW and SP) presented a higher (P < 0·05) apparent viscosity than W and S yogurts.
27845025	5	36	theme	fat	918:920	arg1	SP					958:959	SP	958:959	SP	958:959	All fat replacer treatments (SI, SM, SW and SP) presented a higher (P < 0·05) apparent viscosity than W and S yogurts.
27845025	5	36	theme	fat	918:920	arg1	SW					951:952	SW	951:952	SW	951:952	All fat replacer treatments (SI, SM, SW and SP) presented a higher (P < 0·05) apparent viscosity than W and S yogurts.
27845025	5	36	theme	fat	918:920	arg1	SM					947:948	SM	947:948	SM	947:948	All fat replacer treatments (SI, SM, SW and SP) presented a higher (P < 0·05) apparent viscosity than W and S yogurts.
27845025	5	36	theme	fat	918:920	arg1	SI					943:944	SI	943:944	SI	943:944	All fat replacer treatments (SI, SM, SW and SP) presented a higher (P < 0·05) apparent viscosity than W and S yogurts.
27845025	4	37	theme	yogurt	895:900	arg1	colour					854:859	the colour	850:859	the colour of the low-fat cupuassu goat milk yogurt (P < 0·05)	850:911	The addition of the carbohydrates (inulin and maltodextrin) and proteins (whey protein and skim milk powder) also influenced the colour of the low-fat cupuassu goat milk yogurt (P < 0·05).
27845025	0	38	theme	different	10:18	arg1	replacers					24:32	different fat replacers	10:32	different fat replacers	10:32	Effect of different fat replacers on the physicochemical and instrumental analysis of low-fat cupuassu goat milk yogurts.
27845025	1	39	theme	milk	278:281	arg1	yogurts					283:289	low-fat goat milk yogurts	265:289	low-fat goat milk yogurts prepared with cupuassu pulp by the addition of inulin (SI), maltodextrin (SM), whey protein (SW) and skim milk powder (SP)	265:412	The aim of this Research Communication was to investigate the changes in physicochemical, colour, apparent viscosity and texture properties in low-fat goat milk yogurts prepared with cupuassu pulp by the addition of inulin (SI), maltodextrin (SM), whey protein (SW) and skim milk powder (SP).
27845025	2	40	theme	yogurt	456:461	arg1	batches					421:427	Three batches	415:427	Three batches of each cupuassu goat milk yogurt	415:461	Three batches of each cupuassu goat milk yogurt were prepared and analysed on the 1st day of storage by pH, proximate composition, colour, apparent viscosity, and texture.
27845025	4	41	theme	carbohydrates	745:757	arg1	addition					729:736	The addition	725:736	The addition of the carbohydrates (inulin and maltodextrin) and proteins (whey protein and skim milk powder)	725:832	The addition of the carbohydrates (inulin and maltodextrin) and proteins (whey protein and skim milk powder) also influenced the colour of the low-fat cupuassu goat milk yogurt (P < 0·05).
27845025	4	42	theme	<	905:905	arg1	yogurt					895:900	the low-fat cupuassu goat milk yogurt	864:900	the low-fat cupuassu goat milk yogurt (P < 0·05)	864:911	The addition of the carbohydrates (inulin and maltodextrin) and proteins (whey protein and skim milk powder) also influenced the colour of the low-fat cupuassu goat milk yogurt (P < 0·05).
27845025	4	42	theme	<	905:905	arg1	0·05					907:910	P < 0·05	903:910	P < 0·05	903:910	The addition of the carbohydrates (inulin and maltodextrin) and proteins (whey protein and skim milk powder) also influenced the colour of the low-fat cupuassu goat milk yogurt (P < 0·05).
27845025	2	43	theme	milk	451:454	arg1	yogurt					456:461	each cupuassu goat milk yogurt	432:461	each cupuassu goat milk yogurt	432:461	Three batches of each cupuassu goat milk yogurt were prepared and analysed on the 1st day of storage by pH, proximate composition, colour, apparent viscosity, and texture.
27845025	8	44	theme	whey	1312:1315	arg1	protein					1317:1323	whey protein	1312:1323	whey protein	1312:1323	Furthermore, inulin, maltodextrin, and whey protein can potentially be applied in the goat dairy industry to increase the viscosity of yogurts.
27845025	4	45	theme	P	903:903	arg1	yogurt					895:900	the low-fat cupuassu goat milk yogurt	864:900	the low-fat cupuassu goat milk yogurt (P < 0·05)	864:911	The addition of the carbohydrates (inulin and maltodextrin) and proteins (whey protein and skim milk powder) also influenced the colour of the low-fat cupuassu goat milk yogurt (P < 0·05).
27845025	4	45	theme	P	903:903	arg1	0·05					907:910	P < 0·05	903:910	P < 0·05	903:910	The addition of the carbohydrates (inulin and maltodextrin) and proteins (whey protein and skim milk powder) also influenced the colour of the low-fat cupuassu goat milk yogurt (P < 0·05).
27845025	1	46	theme	physicochemical	195:209	arg1	texture					243:249	texture	243:249	texture	243:249	The aim of this Research Communication was to investigate the changes in physicochemical, colour, apparent viscosity and texture properties in low-fat goat milk yogurts prepared with cupuassu pulp by the addition of inulin (SI), maltodextrin (SM), whey protein (SW) and skim milk powder (SP).
27845025	1	46	theme	physicochemical	195:209	arg1	colour					212:217	colour	212:217	colour	212:217	The aim of this Research Communication was to investigate the changes in physicochemical, colour, apparent viscosity and texture properties in low-fat goat milk yogurts prepared with cupuassu pulp by the addition of inulin (SI), maltodextrin (SM), whey protein (SW) and skim milk powder (SP).
27845025	1	46	theme	physicochemical	195:209	arg1	viscosity					229:237	apparent viscosity	220:237	apparent viscosity	220:237	The aim of this Research Communication was to investigate the changes in physicochemical, colour, apparent viscosity and texture properties in low-fat goat milk yogurts prepared with cupuassu pulp by the addition of inulin (SI), maltodextrin (SM), whey protein (SW) and skim milk powder (SP).
27845025	1	46	theme	physicochemical	195:209	arg1	properties					251:260	physicochemical, colour, apparent viscosity and texture properties	195:260	properties	251:260	The aim of this Research Communication was to investigate the changes in physicochemical, colour, apparent viscosity and texture properties in low-fat goat milk yogurts prepared with cupuassu pulp by the addition of inulin (SI), maltodextrin (SM), whey protein (SW) and skim milk powder (SP).
27845025	0	47	theme	replacers	24:32	arg1	Effect					0:5	Effect	0:5	Effect of different fat replacers on the physicochemical and instrumental analysis of low-fat cupuassu goat milk	0:111	Effect of different fat replacers on the physicochemical and instrumental analysis of low-fat cupuassu goat milk yogurts.
27845025	3	48	theme	milk	638:641	arg1	S					644:644	skimmed milk (S)	630:645	skimmed milk (S)	630:645	In comparison to yogurts from whole (W) or skimmed milk (S), all of the fat replacers improved the physicochemical properties (P < 0·05).
27845025	2	49	theme	goat	446:449	arg1	yogurt					456:461	each cupuassu goat milk yogurt	432:461	each cupuassu goat milk yogurt	432:461	Three batches of each cupuassu goat milk yogurt were prepared and analysed on the 1st day of storage by pH, proximate composition, colour, apparent viscosity, and texture.
27845025	5	50	theme	higher	974:979	arg1	viscosity					1001:1009	a higher (P < 0·05) apparent viscosity	972:1009	a higher (P < 0·05) apparent viscosity than W and S yogurts	972:1030	All fat replacer treatments (SI, SM, SW and SP) presented a higher (P < 0·05) apparent viscosity than W and S yogurts.
27845025	7	51	used	used	1203:1206	arg2	powder					1189:1194	skim milk powder	1179:1194	skim milk powder	1179:1194	These results suggest that skim milk powder can be used to improve the texture properties of low-fat cupuassu goat milk.
27845025	7	52	theme	milk	1184:1187	arg1	powder					1189:1194	skim milk powder	1179:1194	skim milk powder	1179:1194	These results suggest that skim milk powder can be used to improve the texture properties of low-fat cupuassu goat milk.
27845025	0	53	theme	physicochemical	41:55	arg1	analysis					74:81	the physicochemical and instrumental analysis	37:81	the physicochemical and instrumental analysis of low-fat cupuassu goat milk	37:111	Effect of different fat replacers on the physicochemical and instrumental analysis of low-fat cupuassu goat milk yogurts.
27845025	2	54	theme	cupuassu	437:444	arg1	yogurt					456:461	each cupuassu goat milk yogurt	432:461	each cupuassu goat milk yogurt	432:461	Three batches of each cupuassu goat milk yogurt were prepared and analysed on the 1st day of storage by pH, proximate composition, colour, apparent viscosity, and texture.
27845025	3	55	from	S	644:644	arg1	yogurts					604:610	yogurts	604:610	yogurts from whole (W) or skimmed milk (S)	604:645	In comparison to yogurts from whole (W) or skimmed milk (S), all of the fat replacers improved the physicochemical properties (P < 0·05).
27845025	6	56	theme	milk	1068:1071	arg1	powder					1073:1078	skim milk powder	1063:1078	skim milk powder	1063:1078	However, only the addition of skim milk powder increased the texture parameters (firmness and consistency) (P < 0·05).
27845025	8	57	theme	yogurts	1408:1414	arg1	viscosity					1395:1403	the viscosity	1391:1403	the viscosity of yogurts	1391:1414	Furthermore, inulin, maltodextrin, and whey protein can potentially be applied in the goat dairy industry to increase the viscosity of yogurts.
27845025	4	58	theme	skim	816:819	arg1	proteins					789:796	proteins	789:796	proteins (whey protein and skim milk powder)	789:832	The addition of the carbohydrates (inulin and maltodextrin) and proteins (whey protein and skim milk powder) also influenced the colour of the low-fat cupuassu goat milk yogurt (P < 0·05).
27845025	4	58	theme	skim	816:819	arg1	milk					821:824	skim milk	816:824	skim milk	816:824	The addition of the carbohydrates (inulin and maltodextrin) and proteins (whey protein and skim milk powder) also influenced the colour of the low-fat cupuassu goat milk yogurt (P < 0·05).
27845025	1	59	theme	cupuassu	305:312	arg1	pulp					314:317	cupuassu pulp	305:317	cupuassu pulp	305:317	The aim of this Research Communication was to investigate the changes in physicochemical, colour, apparent viscosity and texture properties in low-fat goat milk yogurts prepared with cupuassu pulp by the addition of inulin (SI), maltodextrin (SM), whey protein (SW) and skim milk powder (SP).
27845025	1	60	dep	properties	251:260	arg1	texture					243:249	texture	243:249	texture	243:249	The aim of this Research Communication was to investigate the changes in physicochemical, colour, apparent viscosity and texture properties in low-fat goat milk yogurts prepared with cupuassu pulp by the addition of inulin (SI), maltodextrin (SM), whey protein (SW) and skim milk powder (SP).
27845025	1	60	dep	properties	251:260	arg1	colour					212:217	colour	212:217	colour	212:217	The aim of this Research Communication was to investigate the changes in physicochemical, colour, apparent viscosity and texture properties in low-fat goat milk yogurts prepared with cupuassu pulp by the addition of inulin (SI), maltodextrin (SM), whey protein (SW) and skim milk powder (SP).
27845025	1	60	dep	properties	251:260	arg1	viscosity					229:237	apparent viscosity	220:237	apparent viscosity	220:237	The aim of this Research Communication was to investigate the changes in physicochemical, colour, apparent viscosity and texture properties in low-fat goat milk yogurts prepared with cupuassu pulp by the addition of inulin (SI), maltodextrin (SM), whey protein (SW) and skim milk powder (SP).
27845025	1	60	dep	properties	251:260	arg1	properties					251:260	physicochemical, colour, apparent viscosity and texture properties	195:260	properties	251:260	The aim of this Research Communication was to investigate the changes in physicochemical, colour, apparent viscosity and texture properties in low-fat goat milk yogurts prepared with cupuassu pulp by the addition of inulin (SI), maltodextrin (SM), whey protein (SW) and skim milk powder (SP).
27845025	1	61	from	changes	184:190	arg1	texture					243:249	texture	243:249	texture	243:249	The aim of this Research Communication was to investigate the changes in physicochemical, colour, apparent viscosity and texture properties in low-fat goat milk yogurts prepared with cupuassu pulp by the addition of inulin (SI), maltodextrin (SM), whey protein (SW) and skim milk powder (SP).
27845025	1	61	from	changes	184:190	arg1	colour					212:217	colour	212:217	colour	212:217	The aim of this Research Communication was to investigate the changes in physicochemical, colour, apparent viscosity and texture properties in low-fat goat milk yogurts prepared with cupuassu pulp by the addition of inulin (SI), maltodextrin (SM), whey protein (SW) and skim milk powder (SP).
27845025	1	61	from	changes	184:190	arg1	yogurts					283:289	low-fat goat milk yogurts	265:289	low-fat goat milk yogurts prepared with cupuassu pulp by the addition of inulin (SI), maltodextrin (SM), whey protein (SW) and skim milk powder (SP)	265:412	The aim of this Research Communication was to investigate the changes in physicochemical, colour, apparent viscosity and texture properties in low-fat goat milk yogurts prepared with cupuassu pulp by the addition of inulin (SI), maltodextrin (SM), whey protein (SW) and skim milk powder (SP).
27845025	1	61	from	changes	184:190	arg1	properties					251:260	physicochemical, colour, apparent viscosity and texture properties	195:260	properties	251:260	The aim of this Research Communication was to investigate the changes in physicochemical, colour, apparent viscosity and texture properties in low-fat goat milk yogurts prepared with cupuassu pulp by the addition of inulin (SI), maltodextrin (SM), whey protein (SW) and skim milk powder (SP).
27845025	1	61	from	changes	184:190	arg1	viscosity					229:237	apparent viscosity	220:237	apparent viscosity	220:237	The aim of this Research Communication was to investigate the changes in physicochemical, colour, apparent viscosity and texture properties in low-fat goat milk yogurts prepared with cupuassu pulp by the addition of inulin (SI), maltodextrin (SM), whey protein (SW) and skim milk powder (SP).
27845025	3	62	theme	physicochemical	686:700	arg1	properties					702:711	the physicochemical properties	682:711	the physicochemical properties (P < 0·05)	682:722	In comparison to yogurts from whole (W) or skimmed milk (S), all of the fat replacers improved the physicochemical properties (P < 0·05).
27845025	3	62	theme	physicochemical	686:700	arg1	0·05					718:721	P < 0·05	714:721	P < 0·05	714:721	In comparison to yogurts from whole (W) or skimmed milk (S), all of the fat replacers improved the physicochemical properties (P < 0·05).
27845025	3	63	theme	skimmed	630:636	arg1	S					644:644	skimmed milk (S)	630:645	skimmed milk (S)	630:645	In comparison to yogurts from whole (W) or skimmed milk (S), all of the fat replacers improved the physicochemical properties (P < 0·05).
27845025	4	64	dep	proteins	789:796	arg1	proteins					789:796	proteins	789:796	proteins (whey protein and skim milk powder)	789:832	The addition of the carbohydrates (inulin and maltodextrin) and proteins (whey protein and skim milk powder) also influenced the colour of the low-fat cupuassu goat milk yogurt (P < 0·05).
27845025	4	64	dep	proteins	789:796	arg1	protein					804:810	whey protein	799:810	whey protein	799:810	The addition of the carbohydrates (inulin and maltodextrin) and proteins (whey protein and skim milk powder) also influenced the colour of the low-fat cupuassu goat milk yogurt (P < 0·05).
27845025	4	64	dep	proteins	789:796	arg1	milk					821:824	skim milk	816:824	skim milk	816:824	The addition of the carbohydrates (inulin and maltodextrin) and proteins (whey protein and skim milk powder) also influenced the colour of the low-fat cupuassu goat milk yogurt (P < 0·05).
27845025	5	65	dep	higher	974:979	arg1	0·05					986:989	P < 0·05	982:989	P < 0·05	982:989	All fat replacer treatments (SI, SM, SW and SP) presented a higher (P < 0·05) apparent viscosity than W and S yogurts.
27845025	4	66	theme	cupuassu	876:883	arg1	yogurt					895:900	the low-fat cupuassu goat milk yogurt	864:900	the low-fat cupuassu goat milk yogurt (P < 0·05)	864:911	The addition of the carbohydrates (inulin and maltodextrin) and proteins (whey protein and skim milk powder) also influenced the colour of the low-fat cupuassu goat milk yogurt (P < 0·05).
27845025	4	66	theme	cupuassu	876:883	arg1	0·05					907:910	P < 0·05	903:910	P < 0·05	903:910	The addition of the carbohydrates (inulin and maltodextrin) and proteins (whey protein and skim milk powder) also influenced the colour of the low-fat cupuassu goat milk yogurt (P < 0·05).
27845025	7	67	theme	goat	1262:1265	arg1	milk					1267:1270	low-fat cupuassu goat milk	1245:1270	low-fat cupuassu goat milk	1245:1270	These results suggest that skim milk powder can be used to improve the texture properties of low-fat cupuassu goat milk.
27845025	1	68	theme	whey	370:373	arg1	SP					410:411	SP	410:411	SP	410:411	The aim of this Research Communication was to investigate the changes in physicochemical, colour, apparent viscosity and texture properties in low-fat goat milk yogurts prepared with cupuassu pulp by the addition of inulin (SI), maltodextrin (SM), whey protein (SW) and skim milk powder (SP).
27845025	1	68	theme	whey	370:373	arg1	SW					384:385	SW	384:385	SW	384:385	The aim of this Research Communication was to investigate the changes in physicochemical, colour, apparent viscosity and texture properties in low-fat goat milk yogurts prepared with cupuassu pulp by the addition of inulin (SI), maltodextrin (SM), whey protein (SW) and skim milk powder (SP).
27845025	1	68	theme	whey	370:373	arg1	inulin					338:343	inulin	338:343	inulin (SI)	338:348	The aim of this Research Communication was to investigate the changes in physicochemical, colour, apparent viscosity and texture properties in low-fat goat milk yogurts prepared with cupuassu pulp by the addition of inulin (SI), maltodextrin (SM), whey protein (SW) and skim milk powder (SP).
27845025	1	68	theme	whey	370:373	arg1	protein					375:381	whey protein	370:381	whey protein (SW)	370:386	The aim of this Research Communication was to investigate the changes in physicochemical, colour, apparent viscosity and texture properties in low-fat goat milk yogurts prepared with cupuassu pulp by the addition of inulin (SI), maltodextrin (SM), whey protein (SW) and skim milk powder (SP).
27845025	6	69	theme	texture	1094:1100	arg1	firmness					1114:1121	firmness	1114:1121	firmness	1114:1121	However, only the addition of skim milk powder increased the texture parameters (firmness and consistency) (P < 0·05).
27845025	6	69	theme	texture	1094:1100	arg1	parameters					1102:1111	the texture parameters	1090:1111	the texture parameters (firmness and consistency) (P < 0·05)	1090:1149	However, only the addition of skim milk powder increased the texture parameters (firmness and consistency) (P < 0·05).
27845025	6	69	theme	texture	1094:1100	arg1	0·05					1145:1148	P < 0·05	1141:1148	P < 0·05	1141:1148	However, only the addition of skim milk powder increased the texture parameters (firmness and consistency) (P < 0·05).
27845025	6	69	theme	texture	1094:1100	arg1	consistency					1127:1137	consistency	1127:1137	consistency	1127:1137	However, only the addition of skim milk powder increased the texture parameters (firmness and consistency) (P < 0·05).
27845025	5	70	theme	S	1022:1022	arg1	yogurts					1024:1030	W and S yogurts	1016:1030	W and S yogurts	1016:1030	All fat replacer treatments (SI, SM, SW and SP) presented a higher (P < 0·05) apparent viscosity than W and S yogurts.
27845025	4	71	theme	milk	890:893	arg1	yogurt					895:900	the low-fat cupuassu goat milk yogurt	864:900	the low-fat cupuassu goat milk yogurt (P < 0·05)	864:911	The addition of the carbohydrates (inulin and maltodextrin) and proteins (whey protein and skim milk powder) also influenced the colour of the low-fat cupuassu goat milk yogurt (P < 0·05).
27845025	4	71	theme	milk	890:893	arg1	0·05					907:910	P < 0·05	903:910	P < 0·05	903:910	The addition of the carbohydrates (inulin and maltodextrin) and proteins (whey protein and skim milk powder) also influenced the colour of the low-fat cupuassu goat milk yogurt (P < 0·05).
27845025	7	72	theme	milk	1267:1270	arg1	properties					1231:1240	the texture properties	1219:1240	the texture properties of low-fat cupuassu goat milk	1219:1270	These results suggest that skim milk powder can be used to improve the texture properties of low-fat cupuassu goat milk.
27845025	0	73	theme	instrumental	61:72	arg1	analysis					74:81	the physicochemical and instrumental analysis	37:81	the physicochemical and instrumental analysis of low-fat cupuassu goat milk	37:111	Effect of different fat replacers on the physicochemical and instrumental analysis of low-fat cupuassu goat milk yogurts.
27845025	6	74	theme	<	1143:1143	arg1	parameters					1102:1111	the texture parameters	1090:1111	the texture parameters (firmness and consistency) (P < 0·05)	1090:1149	However, only the addition of skim milk powder increased the texture parameters (firmness and consistency) (P < 0·05).
27845025	6	74	theme	<	1143:1143	arg1	0·05					1145:1148	P < 0·05	1141:1148	P < 0·05	1141:1148	However, only the addition of skim milk powder increased the texture parameters (firmness and consistency) (P < 0·05).
27845025	5	75	theme	P	982:982	arg1	0·05					986:989	P < 0·05	982:989	P < 0·05	982:989	All fat replacer treatments (SI, SM, SW and SP) presented a higher (P < 0·05) apparent viscosity than W and S yogurts.
27845025	4	76	theme	goat	885:888	arg1	yogurt					895:900	the low-fat cupuassu goat milk yogurt	864:900	the low-fat cupuassu goat milk yogurt (P < 0·05)	864:911	The addition of the carbohydrates (inulin and maltodextrin) and proteins (whey protein and skim milk powder) also influenced the colour of the low-fat cupuassu goat milk yogurt (P < 0·05).
27845025	4	76	theme	goat	885:888	arg1	0·05					907:910	P < 0·05	903:910	P < 0·05	903:910	The addition of the carbohydrates (inulin and maltodextrin) and proteins (whey protein and skim milk powder) also influenced the colour of the low-fat cupuassu goat milk yogurt (P < 0·05).
27845025	1	77	theme	apparent	220:227	arg1	viscosity					229:237	apparent viscosity	220:237	apparent viscosity	220:237	The aim of this Research Communication was to investigate the changes in physicochemical, colour, apparent viscosity and texture properties in low-fat goat milk yogurts prepared with cupuassu pulp by the addition of inulin (SI), maltodextrin (SM), whey protein (SW) and skim milk powder (SP).
27845025	1	77	theme	apparent	220:227	arg1	properties					251:260	physicochemical, colour, apparent viscosity and texture properties	195:260	properties	251:260	The aim of this Research Communication was to investigate the changes in physicochemical, colour, apparent viscosity and texture properties in low-fat goat milk yogurts prepared with cupuassu pulp by the addition of inulin (SI), maltodextrin (SM), whey protein (SW) and skim milk powder (SP).
27845025	5	78	theme	replacer	922:929	arg1	treatments					931:940	All fat replacer treatments	914:940	All fat replacer treatments (SI, SM, SW and SP)	914:960	All fat replacer treatments (SI, SM, SW and SP) presented a higher (P < 0·05) apparent viscosity than W and S yogurts.
27845025	5	78	theme	replacer	922:929	arg1	SP					958:959	SP	958:959	SP	958:959	All fat replacer treatments (SI, SM, SW and SP) presented a higher (P < 0·05) apparent viscosity than W and S yogurts.
27845025	5	78	theme	replacer	922:929	arg1	SW					951:952	SW	951:952	SW	951:952	All fat replacer treatments (SI, SM, SW and SP) presented a higher (P < 0·05) apparent viscosity than W and S yogurts.
27845025	5	78	theme	replacer	922:929	arg1	SM					947:948	SM	947:948	SM	947:948	All fat replacer treatments (SI, SM, SW and SP) presented a higher (P < 0·05) apparent viscosity than W and S yogurts.
27845025	5	78	theme	replacer	922:929	arg1	SI					943:944	SI	943:944	SI	943:944	All fat replacer treatments (SI, SM, SW and SP) presented a higher (P < 0·05) apparent viscosity than W and S yogurts.
27845025	5	79	dep	treatments	931:940	arg1	SP					958:959	SP	958:959	SP	958:959	All fat replacer treatments (SI, SM, SW and SP) presented a higher (P < 0·05) apparent viscosity than W and S yogurts.
27845025	5	79	dep	treatments	931:940	arg1	treatments					931:940	All fat replacer treatments	914:940	All fat replacer treatments (SI, SM, SW and SP)	914:960	All fat replacer treatments (SI, SM, SW and SP) presented a higher (P < 0·05) apparent viscosity than W and S yogurts.
27845025	5	79	dep	treatments	931:940	arg1	SW					951:952	SW	951:952	SW	951:952	All fat replacer treatments (SI, SM, SW and SP) presented a higher (P < 0·05) apparent viscosity than W and S yogurts.
27845025	5	79	dep	treatments	931:940	arg1	SM					947:948	SM	947:948	SM	947:948	All fat replacer treatments (SI, SM, SW and SP) presented a higher (P < 0·05) apparent viscosity than W and S yogurts.
27845025	5	79	dep	treatments	931:940	arg1	SI					943:944	SI	943:944	SI	943:944	All fat replacer treatments (SI, SM, SW and SP) presented a higher (P < 0·05) apparent viscosity than W and S yogurts.
27845025	6	80	theme	P	1141:1141	arg1	parameters					1102:1111	the texture parameters	1090:1111	the texture parameters (firmness and consistency) (P < 0·05)	1090:1149	However, only the addition of skim milk powder increased the texture parameters (firmness and consistency) (P < 0·05).
27845025	6	80	theme	P	1141:1141	arg1	0·05					1145:1148	P < 0·05	1141:1148	P < 0·05	1141:1148	However, only the addition of skim milk powder increased the texture parameters (firmness and consistency) (P < 0·05).
27845025	3	81	theme	<	716:716	arg1	properties					702:711	the physicochemical properties	682:711	the physicochemical properties (P < 0·05)	682:722	In comparison to yogurts from whole (W) or skimmed milk (S), all of the fat replacers improved the physicochemical properties (P < 0·05).
27845025	3	81	theme	<	716:716	arg1	0·05					718:721	P < 0·05	714:721	P < 0·05	714:721	In comparison to yogurts from whole (W) or skimmed milk (S), all of the fat replacers improved the physicochemical properties (P < 0·05).
27845025	4	82	dep	carbohydrates	745:757	arg1	maltodextrin					771:782	maltodextrin	771:782	maltodextrin	771:782	The addition of the carbohydrates (inulin and maltodextrin) and proteins (whey protein and skim milk powder) also influenced the colour of the low-fat cupuassu goat milk yogurt (P < 0·05).
27845025	4	82	dep	carbohydrates	745:757	arg1	inulin					760:765	inulin	760:765	inulin	760:765	The addition of the carbohydrates (inulin and maltodextrin) and proteins (whey protein and skim milk powder) also influenced the colour of the low-fat cupuassu goat milk yogurt (P < 0·05).
27845025	4	82	dep	carbohydrates	745:757	arg1	carbohydrates					745:757	the carbohydrates	741:757	the carbohydrates (inulin and maltodextrin)	741:783	The addition of the carbohydrates (inulin and maltodextrin) and proteins (whey protein and skim milk powder) also influenced the colour of the low-fat cupuassu goat milk yogurt (P < 0·05).
27388010	10	0	theme	uronic	1567:1572	arg1	residue					1579:1585	a 3-O-sulfated uronic acid residue	1552:1585	a 3-O-sulfated uronic acid residue	1552:1585	Two novel structures, including a trisaccharide containing two glucosamine residues and a tetrasaccharide containing a 3-O-sulfated uronic acid residue, were discovered.
27388010	8	1	theme	triple	1213:1218	arg1	spectrometer					1236:1247	a triple quadrupole mass spectrometer	1211:1247	a triple quadrupole mass spectrometer operating under the multiple reaction monitoring mode	1211:1301	Satisfactory separation, identification, and quantitation were achieved by coupling hydrophilic interaction chromatography with a triple quadrupole mass spectrometer operating under the multiple reaction monitoring mode.
27388010	5	2	theme	powerful	638:645	arg1	approach					647:654	a powerful approach	636:654	a powerful approach to reveal the building blocks that make up the LMWHs, which are the mutual consequence of the heparin starting materials and the manufacturing process	636:805	Compositional analysis provides a powerful approach to reveal the building blocks that make up the LMWHs, which are the mutual consequence of the heparin starting materials and the manufacturing process.
27388010	8	3	with	chromatography	1191:1204	arg1	spectrometer					1236:1247	a triple quadrupole mass spectrometer	1211:1247	a triple quadrupole mass spectrometer operating under the multiple reaction monitoring mode	1211:1301	Satisfactory separation, identification, and quantitation were achieved by coupling hydrophilic interaction chromatography with a triple quadrupole mass spectrometer operating under the multiple reaction monitoring mode.
27388010	1	4	theme	anticoagulant	159:171	arg1	heparins					126:133	Low-molecular weight heparins	105:133	Low-molecular weight heparins (LMWHs)	105:141	Low-molecular weight heparins (LMWHs) are widely used anticoagulant drugs.
27388010	1	4	theme	anticoagulant	159:171	arg1	drugs					173:177	widely used anticoagulant drugs	147:177	widely used anticoagulant drugs	147:177	Low-molecular weight heparins (LMWHs) are widely used anticoagulant drugs.
27388010	9	5	theme	enzymatic	1310:1318	arg1	digestion					1320:1328	enzymatic digestion	1310:1328	enzymatic digestion	1310:1328	After enzymatic digestion, over 30 species were detected, with both natural and chemically modified heparin basic building blocks.
27388010	9	6	theme	basic	1412:1416	arg1	blocks					1427:1432	chemically modified heparin basic building blocks	1384:1432	chemically modified heparin basic building blocks	1384:1432	After enzymatic digestion, over 30 species were detected, with both natural and chemically modified heparin basic building blocks.
27388010	0	7	theme	Weight	89:94	arg1	Heparin					96:102	Low-Molecular Weight Heparin	75:102	Low-Molecular Weight Heparin	75:102	Comprehensive Identification and Quantitation of Basic Building Blocks for Low-Molecular Weight Heparin.
27388010	6	8	theme	building	887:894	arg1	blocks					896:901	the most basic building blocks	872:901	the most basic building blocks of LMWHs	872:910	Here, we introduce a comprehensive analytical method to recover the most basic building blocks of LMWHs.
27388010	5	9	theme	materials	767:775	arg1	consequence					731:741	the mutual consequence	720:741	the mutual consequence of the heparin starting materials	720:775	Compositional analysis provides a powerful approach to reveal the building blocks that make up the LMWHs, which are the mutual consequence of the heparin starting materials and the manufacturing process.
27388010	5	9	theme	materials	767:775	arg1	process					799:805	the manufacturing process	781:805	the manufacturing process	781:805	Compositional analysis provides a powerful approach to reveal the building blocks that make up the LMWHs, which are the mutual consequence of the heparin starting materials and the manufacturing process.
27388010	5	9	theme	materials	767:775	arg1	LMWHs					703:707	the LMWHs	699:707	the LMWHs	699:707	Compositional analysis provides a powerful approach to reveal the building blocks that make up the LMWHs, which are the mutual consequence of the heparin starting materials and the manufacturing process.
27388010	9	10	theme	building	1418:1425	arg1	blocks					1427:1432	chemically modified heparin basic building blocks	1384:1432	chemically modified heparin basic building blocks	1384:1432	After enzymatic digestion, over 30 species were detected, with both natural and chemically modified heparin basic building blocks.
27388010	6	11	theme	basic	881:885	arg1	blocks					896:901	the most basic building blocks	872:901	the most basic building blocks of LMWHs	872:910	Here, we introduce a comprehensive analytical method to recover the most basic building blocks of LMWHs.
27388010	10	12	theme	novel	1439:1443	arg1	structures					1445:1454	Two novel structures	1435:1454	Two novel structures	1435:1454	Two novel structures, including a trisaccharide containing two glucosamine residues and a tetrasaccharide containing a 3-O-sulfated uronic acid residue, were discovered.
27388010	10	12	theme	novel	1439:1443	arg1	tetrasaccharide					1525:1539	a tetrasaccharide	1523:1539	a tetrasaccharide containing a 3-O-sulfated uronic acid residue	1523:1585	Two novel structures, including a trisaccharide containing two glucosamine residues and a tetrasaccharide containing a 3-O-sulfated uronic acid residue, were discovered.
27388010	10	12	theme	novel	1439:1443	arg1	trisaccharide					1469:1481	a trisaccharide	1467:1481	a trisaccharide containing two glucosamine residues	1467:1517	Two novel structures, including a trisaccharide containing two glucosamine residues and a tetrasaccharide containing a 3-O-sulfated uronic acid residue, were discovered.
27388010	7	13	theme	enzymatic	942:950	arg1	digestion					952:960	enzymatic digestion	942:960	enzymatic digestion	942:960	A strategy of combining both enzymatic digestion and oxidative degradation of LMWH was used to make the NRE, RE, and backbone structures differentiable from one another.
27388010	2	14	theme	nonreducing	310:320	arg1	NRE					327:329	NRE	327:329	NRE	327:329	They inherit the heterogeneous backbone sequences of the parent heparin, while the chemical depolymerization process modifies the nonreducing end (NRE) and reducing end (RE) of their sugar chains.
27388010	2	14	theme	nonreducing	310:320	arg1	end					322:324	the nonreducing end	306:324	the nonreducing end (NRE)	306:330	They inherit the heterogeneous backbone sequences of the parent heparin, while the chemical depolymerization process modifies the nonreducing end (NRE) and reducing end (RE) of their sugar chains.
27388010	4	15	theme	drug	579:582	arg1	quality					584:590	drug quality	579:590	drug quality	579:590	It is important to precisely characterize the structures of LMWHs, especially their chemical modifications, to ensure drug quality and safety.
27388010	6	16	theme	most	876:879	arg1	blocks					896:901	the most basic building blocks	872:901	the most basic building blocks of LMWHs	872:910	Here, we introduce a comprehensive analytical method to recover the most basic building blocks of LMWHs.
27388010	11	17	theme	degraded	1629:1636	arg1	samples					1638:1644	Reduced and oxidatively degraded samples	1605:1644	Reduced and oxidatively degraded samples	1605:1644	Reduced and oxidatively degraded samples were analyzed to provide the complementary information on both termini of LMWHs.
27388010	10	18	contain	containing	1541:1550	arg1	tetrasaccharide					1525:1539	a tetrasaccharide	1523:1539	a tetrasaccharide containing a 3-O-sulfated uronic acid residue	1523:1585	Two novel structures, including a trisaccharide containing two glucosamine residues and a tetrasaccharide containing a 3-O-sulfated uronic acid residue, were discovered.
27388010	10	18	contain	containing	1541:1550	arg2	residue					1579:1585	a 3-O-sulfated uronic acid residue	1552:1585	a 3-O-sulfated uronic acid residue	1552:1585	Two novel structures, including a trisaccharide containing two glucosamine residues and a tetrasaccharide containing a 3-O-sulfated uronic acid residue, were discovered.
27388010	8	19	theme	coupling	1158:1165	arg1	chromatography					1191:1204	coupling hydrophilic interaction chromatography	1158:1204	coupling hydrophilic interaction chromatography with a triple quadrupole mass spectrometer operating under the multiple reaction monitoring mode	1158:1301	Satisfactory separation, identification, and quantitation were achieved by coupling hydrophilic interaction chromatography with a triple quadrupole mass spectrometer operating under the multiple reaction monitoring mode.
27388010	5	20	theme	Compositional	604:616	arg1	analysis					618:625	Compositional analysis	604:625	Compositional analysis	604:625	Compositional analysis provides a powerful approach to reveal the building blocks that make up the LMWHs, which are the mutual consequence of the heparin starting materials and the manufacturing process.
27388010	7	21	theme	differentiable	1050:1063	arg1	structures					1039:1048	the NRE, RE, and backbone structures	1013:1048	the NRE, RE, and backbone structures differentiable from one another	1013:1080	A strategy of combining both enzymatic digestion and oxidative degradation of LMWH was used to make the NRE, RE, and backbone structures differentiable from one another.
27388010	11	22	from	information	1689:1699	arg1	termini					1709:1715	both termini	1704:1715	both termini of LMWHs	1704:1724	Reduced and oxidatively degraded samples were analyzed to provide the complementary information on both termini of LMWHs.
27388010	5	23	theme	heparin	750:756	arg1	materials					767:775	the heparin starting materials	746:775	the heparin starting materials	746:775	Compositional analysis provides a powerful approach to reveal the building blocks that make up the LMWHs, which are the mutual consequence of the heparin starting materials and the manufacturing process.
27388010	5	24	theme	manufacturing	785:797	arg1	process					799:805	the manufacturing process	781:805	the manufacturing process	781:805	Compositional analysis provides a powerful approach to reveal the building blocks that make up the LMWHs, which are the mutual consequence of the heparin starting materials and the manufacturing process.
27388010	8	25	theme	quadrupole	1220:1229	arg1	spectrometer					1236:1247	a triple quadrupole mass spectrometer	1211:1247	a triple quadrupole mass spectrometer operating under the multiple reaction monitoring mode	1211:1301	Satisfactory separation, identification, and quantitation were achieved by coupling hydrophilic interaction chromatography with a triple quadrupole mass spectrometer operating under the multiple reaction monitoring mode.
27388010	7	26	dep	one	1070:1072	arg1	another					1074:1080	another	1074:1080	another	1074:1080	A strategy of combining both enzymatic digestion and oxidative degradation of LMWH was used to make the NRE, RE, and backbone structures differentiable from one another.
27388010	10	27	theme	3-O-sulfated	1554:1565	arg1	residue					1579:1585	a 3-O-sulfated uronic acid residue	1552:1585	a 3-O-sulfated uronic acid residue	1552:1585	Two novel structures, including a trisaccharide containing two glucosamine residues and a tetrasaccharide containing a 3-O-sulfated uronic acid residue, were discovered.
27388010	7	28	theme	backbone	1030:1037	arg1	structures					1039:1048	the NRE, RE, and backbone structures	1013:1048	the NRE, RE, and backbone structures differentiable from one another	1013:1080	A strategy of combining both enzymatic digestion and oxidative degradation of LMWH was used to make the NRE, RE, and backbone structures differentiable from one another.
27388010	5	29	theme	mutual	724:729	arg1	consequence					731:741	the mutual consequence	720:741	the mutual consequence of the heparin starting materials	720:775	Compositional analysis provides a powerful approach to reveal the building blocks that make up the LMWHs, which are the mutual consequence of the heparin starting materials and the manufacturing process.
27388010	5	29	theme	mutual	724:729	arg1	LMWHs					703:707	the LMWHs	699:707	the LMWHs	699:707	Compositional analysis provides a powerful approach to reveal the building blocks that make up the LMWHs, which are the mutual consequence of the heparin starting materials and the manufacturing process.
27388010	7	30	theme	NRE	1017:1019	arg1	structures					1039:1048	the NRE, RE, and backbone structures	1013:1048	the NRE, RE, and backbone structures differentiable from one another	1013:1080	A strategy of combining both enzymatic digestion and oxidative degradation of LMWH was used to make the NRE, RE, and backbone structures differentiable from one another.
27388010	2	31	theme	chains	369:374	arg1	end					345:347	reducing end	336:347	reducing end (RE)	336:352	They inherit the heterogeneous backbone sequences of the parent heparin, while the chemical depolymerization process modifies the nonreducing end (NRE) and reducing end (RE) of their sugar chains.
27388010	2	31	theme	chains	369:374	arg1	NRE					327:329	NRE	327:329	NRE	327:329	They inherit the heterogeneous backbone sequences of the parent heparin, while the chemical depolymerization process modifies the nonreducing end (NRE) and reducing end (RE) of their sugar chains.
27388010	2	31	theme	chains	369:374	arg1	RE					350:351	RE	350:351	RE	350:351	They inherit the heterogeneous backbone sequences of the parent heparin, while the chemical depolymerization process modifies the nonreducing end (NRE) and reducing end (RE) of their sugar chains.
27388010	2	31	theme	chains	369:374	arg1	end					322:324	the nonreducing end	306:324	the nonreducing end (NRE)	306:330	They inherit the heterogeneous backbone sequences of the parent heparin, while the chemical depolymerization process modifies the nonreducing end (NRE) and reducing end (RE) of their sugar chains.
27388010	0	32	theme	Comprehensive	0:12	arg1	Identification					14:27	Identification	14:27	Identification	14:27	Comprehensive Identification and Quantitation of Basic Building Blocks for Low-Molecular Weight Heparin.
27388010	8	33	theme	mass	1231:1234	arg1	spectrometer					1236:1247	a triple quadrupole mass spectrometer	1211:1247	a triple quadrupole mass spectrometer operating under the multiple reaction monitoring mode	1211:1301	Satisfactory separation, identification, and quantitation were achieved by coupling hydrophilic interaction chromatography with a triple quadrupole mass spectrometer operating under the multiple reaction monitoring mode.
27388010	12	34	theme	enoxaparin	1781:1790	arg1	injections					1792:1801	enoxaparin injections	1781:1801	enoxaparin injections	1781:1801	The reproducibility of this method was evaluated, and enoxaparin injections were analyzed to demonstrate the application of this method for evaluating the sameness of LMWH products.
27388010	1	35	theme	Low-molecular	105:117	arg1	drugs					173:177	widely used anticoagulant drugs	147:177	widely used anticoagulant drugs	147:177	Low-molecular weight heparins (LMWHs) are widely used anticoagulant drugs.
27388010	1	35	theme	Low-molecular	105:117	arg1	LMWHs					136:140	LMWHs	136:140	LMWHs	136:140	Low-molecular weight heparins (LMWHs) are widely used anticoagulant drugs.
27388010	1	35	theme	Low-molecular	105:117	arg1	heparins					126:133	Low-molecular weight heparins	105:133	Low-molecular weight heparins (LMWHs)	105:141	Low-molecular weight heparins (LMWHs) are widely used anticoagulant drugs.
27388010	2	36	theme	sugar	363:367	arg1	chains					369:374	their sugar chains	357:374	their sugar chains	357:374	They inherit the heterogeneous backbone sequences of the parent heparin, while the chemical depolymerization process modifies the nonreducing end (NRE) and reducing end (RE) of their sugar chains.
27388010	8	37	theme	interaction	1179:1189	arg1	chromatography					1191:1204	coupling hydrophilic interaction chromatography	1158:1204	coupling hydrophilic interaction chromatography with a triple quadrupole mass spectrometer operating under the multiple reaction monitoring mode	1158:1301	Satisfactory separation, identification, and quantitation were achieved by coupling hydrophilic interaction chromatography with a triple quadrupole mass spectrometer operating under the multiple reaction monitoring mode.
27388010	1	38	theme	weight	119:124	arg1	drugs					173:177	widely used anticoagulant drugs	147:177	widely used anticoagulant drugs	147:177	Low-molecular weight heparins (LMWHs) are widely used anticoagulant drugs.
27388010	1	38	theme	weight	119:124	arg1	LMWHs					136:140	LMWHs	136:140	LMWHs	136:140	Low-molecular weight heparins (LMWHs) are widely used anticoagulant drugs.
27388010	1	38	theme	weight	119:124	arg1	heparins					126:133	Low-molecular weight heparins	105:133	Low-molecular weight heparins (LMWHs)	105:141	Low-molecular weight heparins (LMWHs) are widely used anticoagulant drugs.
27388010	8	39	theme	hydrophilic	1167:1177	arg1	chromatography					1191:1204	coupling hydrophilic interaction chromatography	1158:1204	coupling hydrophilic interaction chromatography with a triple quadrupole mass spectrometer operating under the multiple reaction monitoring mode	1158:1301	Satisfactory separation, identification, and quantitation were achieved by coupling hydrophilic interaction chromatography with a triple quadrupole mass spectrometer operating under the multiple reaction monitoring mode.
27388010	4	40	theme	modifications	554:566	arg1	structures					507:516	the structures	503:516	the structures of LMWHs, especially their chemical modifications	503:566	It is important to precisely characterize the structures of LMWHs, especially their chemical modifications, to ensure drug quality and safety.
27388010	12	41	theme	products	1899:1906	arg1	sameness					1882:1889	the sameness	1878:1889	the sameness of LMWH products	1878:1906	The reproducibility of this method was evaluated, and enoxaparin injections were analyzed to demonstrate the application of this method for evaluating the sameness of LMWH products.
27388010	3	42	theme	LMWHs	454:458	arg1	complexity					440:449	the structural complexity	425:449	the structural complexity of LMWHs	425:458	Some side reactions may also occur and increase the structural complexity of LMWHs.
27388010	0	43	theme	Basic	49:53	arg1	Blocks					64:69	Basic Building Blocks	49:69	Basic Building Blocks for Low-Molecular Weight Heparin	49:102	Comprehensive Identification and Quantitation of Basic Building Blocks for Low-Molecular Weight Heparin.
27388010	4	44	theme	chemical	545:552	arg1	modifications					554:566	LMWHs, especially their chemical modifications	521:566	modifications	554:566	It is important to precisely characterize the structures of LMWHs, especially their chemical modifications, to ensure drug quality and safety.
27388010	10	45	theme	glucosamine	1498:1508	arg1	residues					1510:1517	two glucosamine residues	1494:1517	two glucosamine residues	1494:1517	Two novel structures, including a trisaccharide containing two glucosamine residues and a tetrasaccharide containing a 3-O-sulfated uronic acid residue, were discovered.
27388010	6	46	theme	analytical	843:852	arg1	method					854:859	a comprehensive analytical method	827:859	a comprehensive analytical method to recover the most basic building blocks of LMWHs	827:910	Here, we introduce a comprehensive analytical method to recover the most basic building blocks of LMWHs.
27388010	3	47	theme	structural	429:438	arg1	complexity					440:449	the structural complexity	425:449	the structural complexity of LMWHs	425:458	Some side reactions may also occur and increase the structural complexity of LMWHs.
27388010	5	48	theme	starting	758:765	arg1	materials					767:775	the heparin starting materials	746:775	the heparin starting materials	746:775	Compositional analysis provides a powerful approach to reveal the building blocks that make up the LMWHs, which are the mutual consequence of the heparin starting materials and the manufacturing process.
27388010	7	49	from	one	1070:1072	arg1	differentiable					1050:1063	differentiable	1050:1063	differentiable	1050:1063	A strategy of combining both enzymatic digestion and oxidative degradation of LMWH was used to make the NRE, RE, and backbone structures differentiable from one another.
27388010	7	50	theme	RE	1022:1023	arg1	structures					1039:1048	the NRE, RE, and backbone structures	1013:1048	the NRE, RE, and backbone structures differentiable from one another	1013:1080	A strategy of combining both enzymatic digestion and oxidative degradation of LMWH was used to make the NRE, RE, and backbone structures differentiable from one another.
27388010	6	51	theme	comprehensive	829:841	arg1	method					854:859	a comprehensive analytical method	827:859	a comprehensive analytical method to recover the most basic building blocks of LMWHs	827:910	Here, we introduce a comprehensive analytical method to recover the most basic building blocks of LMWHs.
27388010	2	52	theme	heparin	244:250	arg1	sequences					220:228	the heterogeneous backbone sequences	193:228	the heterogeneous backbone sequences of the parent heparin	193:250	They inherit the heterogeneous backbone sequences of the parent heparin, while the chemical depolymerization process modifies the nonreducing end (NRE) and reducing end (RE) of their sugar chains.
27388010	12	53	theme	method	1856:1861	arg1	application					1836:1846	the application	1832:1846	the application of this method for evaluating the sameness of LMWH products	1832:1906	The reproducibility of this method was evaluated, and enoxaparin injections were analyzed to demonstrate the application of this method for evaluating the sameness of LMWH products.
27388010	6	54	theme	LMWHs	906:910	arg1	blocks					896:901	the most basic building blocks	872:901	the most basic building blocks of LMWHs	872:910	Here, we introduce a comprehensive analytical method to recover the most basic building blocks of LMWHs.
27388010	0	55	theme	Blocks	64:69	arg1	Quantitation					33:44	Quantitation	33:44	Quantitation	33:44	Comprehensive Identification and Quantitation of Basic Building Blocks for Low-Molecular Weight Heparin.
27388010	0	55	theme	Blocks	64:69	arg1	Identification					14:27	Identification	14:27	Identification	14:27	Comprehensive Identification and Quantitation of Basic Building Blocks for Low-Molecular Weight Heparin.
27388010	10	56	theme	acid	1574:1577	arg1	residue					1579:1585	a 3-O-sulfated uronic acid residue	1552:1585	a 3-O-sulfated uronic acid residue	1552:1585	Two novel structures, including a trisaccharide containing two glucosamine residues and a tetrasaccharide containing a 3-O-sulfated uronic acid residue, were discovered.
27388010	2	57	theme	parent	237:242	arg1	heparin					244:250	the parent heparin	233:250	the parent heparin	233:250	They inherit the heterogeneous backbone sequences of the parent heparin, while the chemical depolymerization process modifies the nonreducing end (NRE) and reducing end (RE) of their sugar chains.
27388010	8	58	theme	Satisfactory	1083:1094	arg1	separation					1096:1105	Satisfactory separation	1083:1105	Satisfactory separation	1083:1105	Satisfactory separation, identification, and quantitation were achieved by coupling hydrophilic interaction chromatography with a triple quadrupole mass spectrometer operating under the multiple reaction monitoring mode.
27388010	0	59	theme	Building	55:62	arg1	Blocks					64:69	Basic Building Blocks	49:69	Basic Building Blocks for Low-Molecular Weight Heparin	49:102	Comprehensive Identification and Quantitation of Basic Building Blocks for Low-Molecular Weight Heparin.
27388010	5	60	theme	building	670:677	arg1	blocks					679:684	the building blocks	666:684	the building blocks that make up the LMWHs, which are the mutual consequence of the heparin starting materials and the manufacturing process	666:805	Compositional analysis provides a powerful approach to reveal the building blocks that make up the LMWHs, which are the mutual consequence of the heparin starting materials and the manufacturing process.
27388010	11	61	theme	Reduced	1605:1611	arg1	samples					1638:1644	Reduced and oxidatively degraded samples	1605:1644	Reduced and oxidatively degraded samples	1605:1644	Reduced and oxidatively degraded samples were analyzed to provide the complementary information on both termini of LMWHs.
27388010	8	62	theme	monitoring	1287:1296	arg1	mode					1298:1301	the multiple reaction monitoring mode	1265:1301	the multiple reaction monitoring mode	1265:1301	Satisfactory separation, identification, and quantitation were achieved by coupling hydrophilic interaction chromatography with a triple quadrupole mass spectrometer operating under the multiple reaction monitoring mode.
27388010	4	63	theme	LMWHs	521:525	arg1	modifications					554:566	LMWHs, especially their chemical modifications	521:566	modifications	554:566	It is important to precisely characterize the structures of LMWHs, especially their chemical modifications, to ensure drug quality and safety.
27388010	8	64	theme	reaction	1278:1285	arg1	mode					1298:1301	the multiple reaction monitoring mode	1265:1301	the multiple reaction monitoring mode	1265:1301	Satisfactory separation, identification, and quantitation were achieved by coupling hydrophilic interaction chromatography with a triple quadrupole mass spectrometer operating under the multiple reaction monitoring mode.
27388010	12	65	theme	LMWH	1894:1897	arg1	products					1899:1906	LMWH products	1894:1906	LMWH products	1894:1906	The reproducibility of this method was evaluated, and enoxaparin injections were analyzed to demonstrate the application of this method for evaluating the sameness of LMWH products.
27388010	2	66	theme	depolymerization	272:287	arg1	process					289:295	the chemical depolymerization process	259:295	the chemical depolymerization process	259:295	They inherit the heterogeneous backbone sequences of the parent heparin, while the chemical depolymerization process modifies the nonreducing end (NRE) and reducing end (RE) of their sugar chains.
27388010	7	67	theme	LMWH	991:994	arg1	digestion					952:960	enzymatic digestion	942:960	enzymatic digestion	942:960	A strategy of combining both enzymatic digestion and oxidative degradation of LMWH was used to make the NRE, RE, and backbone structures differentiable from one another.
27388010	7	67	theme	LMWH	991:994	arg1	degradation					976:986	oxidative degradation	966:986	oxidative degradation of LMWH	966:994	A strategy of combining both enzymatic digestion and oxidative degradation of LMWH was used to make the NRE, RE, and backbone structures differentiable from one another.
27388010	2	68	theme	reducing	336:343	arg1	end					345:347	reducing end	336:347	reducing end (RE)	336:352	They inherit the heterogeneous backbone sequences of the parent heparin, while the chemical depolymerization process modifies the nonreducing end (NRE) and reducing end (RE) of their sugar chains.
27388010	2	68	theme	reducing	336:343	arg1	RE					350:351	RE	350:351	RE	350:351	They inherit the heterogeneous backbone sequences of the parent heparin, while the chemical depolymerization process modifies the nonreducing end (NRE) and reducing end (RE) of their sugar chains.
27388010	8	69	theme	multiple	1269:1276	arg1	mode					1298:1301	the multiple reaction monitoring mode	1265:1301	the multiple reaction monitoring mode	1265:1301	Satisfactory separation, identification, and quantitation were achieved by coupling hydrophilic interaction chromatography with a triple quadrupole mass spectrometer operating under the multiple reaction monitoring mode.
27388010	2	70	theme	backbone	211:218	arg1	sequences					220:228	the heterogeneous backbone sequences	193:228	the heterogeneous backbone sequences of the parent heparin	193:250	They inherit the heterogeneous backbone sequences of the parent heparin, while the chemical depolymerization process modifies the nonreducing end (NRE) and reducing end (RE) of their sugar chains.
27388010	2	71	theme	chemical	263:270	arg1	process					289:295	the chemical depolymerization process	259:295	the chemical depolymerization process	259:295	They inherit the heterogeneous backbone sequences of the parent heparin, while the chemical depolymerization process modifies the nonreducing end (NRE) and reducing end (RE) of their sugar chains.
27388010	7	72	theme	oxidative	966:974	arg1	degradation					976:986	oxidative degradation	966:986	oxidative degradation of LMWH	966:994	A strategy of combining both enzymatic digestion and oxidative degradation of LMWH was used to make the NRE, RE, and backbone structures differentiable from one another.
27388010	7	73	used	used	1000:1003	arg2	strategy					915:922	A strategy	913:922	A strategy of combining both enzymatic digestion and oxidative degradation of LMWH	913:994	A strategy of combining both enzymatic digestion and oxidative degradation of LMWH was used to make the NRE, RE, and backbone structures differentiable from one another.
27388010	0	74	theme	Low-Molecular	75:87	arg1	Heparin					96:102	Low-Molecular Weight Heparin	75:102	Low-Molecular Weight Heparin	75:102	Comprehensive Identification and Quantitation of Basic Building Blocks for Low-Molecular Weight Heparin.
27388010	2	75	theme	heterogeneous	197:209	arg1	sequences					220:228	the heterogeneous backbone sequences	193:228	the heterogeneous backbone sequences of the parent heparin	193:250	They inherit the heterogeneous backbone sequences of the parent heparin, while the chemical depolymerization process modifies the nonreducing end (NRE) and reducing end (RE) of their sugar chains.
27388010	11	76	theme	LMWHs	1720:1724	arg1	termini					1709:1715	both termini	1704:1715	both termini of LMWHs	1704:1724	Reduced and oxidatively degraded samples were analyzed to provide the complementary information on both termini of LMWHs.
27388010	12	77	theme	method	1755:1760	arg1	reproducibility					1731:1745	The reproducibility	1727:1745	The reproducibility of this method	1727:1760	The reproducibility of this method was evaluated, and enoxaparin injections were analyzed to demonstrate the application of this method for evaluating the sameness of LMWH products.
27388010	9	78	theme	modified	1395:1402	arg1	blocks					1427:1432	chemically modified heparin basic building blocks	1384:1432	chemically modified heparin basic building blocks	1384:1432	After enzymatic digestion, over 30 species were detected, with both natural and chemically modified heparin basic building blocks.
27388010	3	79	theme	side	382:385	arg1	reactions					387:395	Some side reactions	377:395	Some side reactions	377:395	Some side reactions may also occur and increase the structural complexity of LMWHs.
27388010	11	80	theme	complementary	1675:1687	arg1	information					1689:1699	the complementary information	1671:1699	the complementary information on both termini of LMWHs	1671:1724	Reduced and oxidatively degraded samples were analyzed to provide the complementary information on both termini of LMWHs.
27388010	10	81	contain	containing	1483:1492	arg1	trisaccharide					1469:1481	a trisaccharide	1467:1481	a trisaccharide containing two glucosamine residues	1467:1517	Two novel structures, including a trisaccharide containing two glucosamine residues and a tetrasaccharide containing a 3-O-sulfated uronic acid residue, were discovered.
27388010	10	81	contain	containing	1483:1492	arg2	residues					1510:1517	two glucosamine residues	1494:1517	two glucosamine residues	1494:1517	Two novel structures, including a trisaccharide containing two glucosamine residues and a tetrasaccharide containing a 3-O-sulfated uronic acid residue, were discovered.
27388010	1	82	theme	used	154:157	arg1	heparins					126:133	Low-molecular weight heparins	105:133	Low-molecular weight heparins (LMWHs)	105:141	Low-molecular weight heparins (LMWHs) are widely used anticoagulant drugs.
27388010	1	82	theme	used	154:157	arg1	drugs					173:177	widely used anticoagulant drugs	147:177	widely used anticoagulant drugs	147:177	Low-molecular weight heparins (LMWHs) are widely used anticoagulant drugs.
27388010	9	83	theme	heparin	1404:1410	arg1	blocks					1427:1432	chemically modified heparin basic building blocks	1384:1432	chemically modified heparin basic building blocks	1384:1432	After enzymatic digestion, over 30 species were detected, with both natural and chemically modified heparin basic building blocks.
25769823	7	0	theme	days	1208:1211	arg1	supplementation					1213:1227	35 days supplementation	1205:1227	35 days supplementation	1205:1227	MOS supplementation altered the bacterial community composition from 7 days supplementation through 35 days supplementation.
25769823	10	1	theme	supplementation	1552:1566	arg1	differences					1458:1468	differences	1458:1468	differences between the functional potentials of the bacterial communities	1458:1531	PICRUSt was used to identify differences between the functional potentials of the bacterial communities as a result of MOS supplementation.
25769823	10	1	theme	supplementation	1552:1566	arg1	result					1538:1543	a result	1536:1543	a result of MOS supplementation	1536:1566	PICRUSt was used to identify differences between the functional potentials of the bacterial communities as a result of MOS supplementation.
25769823	1	2	from	effects	194:200	arg1	structure					306:314	broiler cecal bacterial community structure	272:314	broiler cecal bacterial community structure	272:314	This study focused on identifying reproducible effects of dietary supplementation with a mannan oligosaccharide (MOS) on the broiler cecal bacterial community structure and function in a commercial production setting.
25769823	1	2	from	effects	194:200	arg1	function					320:327	function	320:327	function	320:327	This study focused on identifying reproducible effects of dietary supplementation with a mannan oligosaccharide (MOS) on the broiler cecal bacterial community structure and function in a commercial production setting.
25769823	10	3	theme	MOS	1548:1550	arg1	supplementation					1552:1566	MOS supplementation	1548:1566	MOS supplementation	1548:1566	PICRUSt was used to identify differences between the functional potentials of the bacterial communities as a result of MOS supplementation.
25769823	7	4	theme	MOS	1105:1107	arg1	supplementation					1109:1123	MOS supplementation	1105:1123	MOS supplementation	1105:1123	MOS supplementation altered the bacterial community composition from 7 days supplementation through 35 days supplementation.
25769823	2	5	theme	separate	369:376	arg1	trials					378:383	Two separate trials	365:383	Two separate trials	365:383	Two separate trials, each with a control and a supplemented group, were carried out in the same commercial location and run concurrently.
25769823	2	5	theme	separate	369:376	arg1	each					386:389	each	386:389	each	386:389	Two separate trials, each with a control and a supplemented group, were carried out in the same commercial location and run concurrently.
25769823	1	6	dep	structure	306:314	arg1	the					268:270	the	268:270	the	268:270	This study focused on identifying reproducible effects of dietary supplementation with a mannan oligosaccharide (MOS) on the broiler cecal bacterial community structure and function in a commercial production setting.
25769823	10	7	used	used	1441:1444	arg2	PICRUSt					1429:1435	PICRUSt	1429:1435	PICRUSt	1429:1435	PICRUSt was used to identify differences between the functional potentials of the bacterial communities as a result of MOS supplementation.
25769823	11	8	theme	ortholog	1588:1595	arg1	predictions					1606:1616	level 3 KEGG ortholog function predictions	1575:1616	level 3 KEGG ortholog function predictions	1575:1616	Using level 3 KEGG ortholog function predictions, differences between control and supplemented groups were observed, with very strong segregation noted on day 35 posthatch in both trials.
25769823	1	9	from	function	320:327	arg1	setting					356:362	a commercial production setting	332:362	a commercial production setting	332:362	This study focused on identifying reproducible effects of dietary supplementation with a mannan oligosaccharide (MOS) on the broiler cecal bacterial community structure and function in a commercial production setting.
25769823	3	10	theme	commercial	544:553	arg1	hatchery					555:562	the same commercial hatchery	535:562	the same commercial hatchery	535:562	Approximately 10,000 birds from the same commercial hatchery were mirror imaged into each of four commercial broiler sheds and fed either a control or supplemented diet.
25769823	3	11	from	hatchery	555:562	arg1	birds					524:528	Approximately 10,000 birds	503:528	Approximately 10,000 birds from the same commercial hatchery	503:562	Approximately 10,000 birds from the same commercial hatchery were mirror imaged into each of four commercial broiler sheds and fed either a control or supplemented diet.
25769823	1	12	theme	broiler	272:278	arg1	structure					306:314	broiler cecal bacterial community structure	272:314	broiler cecal bacterial community structure	272:314	This study focused on identifying reproducible effects of dietary supplementation with a mannan oligosaccharide (MOS) on the broiler cecal bacterial community structure and function in a commercial production setting.
25769823	11	13	theme	strong	1696:1701	arg1	segregation					1703:1713	very strong segregation	1691:1713	very strong segregation noted on day 35 posthatch in both trials	1691:1754	Using level 3 KEGG ortholog function predictions, differences between control and supplemented groups were observed, with very strong segregation noted on day 35 posthatch in both trials.
25769823	12	14	theme	MOS	1829:1831	arg1	result					1819:1824	a result	1817:1824	a result of MOS	1817:1831	This indicated that alterations of bacterial communities as a result of MOS are likely to alter the functional capability of the cecum.
25769823	12	15	theme	communities	1802:1812	arg1	alterations					1777:1787	alterations	1777:1787	alterations of bacterial communities as a result of MOS	1777:1831	This indicated that alterations of bacterial communities as a result of MOS are likely to alter the functional capability of the cecum.
25769823	10	16	theme	bacterial	1511:1519	arg1	communities					1521:1531	the bacterial communities	1507:1531	the bacterial communities	1507:1531	PICRUSt was used to identify differences between the functional potentials of the bacterial communities as a result of MOS supplementation.
25769823	12	17	theme	cecum	1886:1890	arg1	capability					1868:1877	the functional capability	1853:1877	the functional capability of the cecum	1853:1890	This indicated that alterations of bacterial communities as a result of MOS are likely to alter the functional capability of the cecum.
25769823	1	18	theme	cecal	280:284	arg1	structure					306:314	broiler cecal bacterial community structure	272:314	broiler cecal bacterial community structure	272:314	This study focused on identifying reproducible effects of dietary supplementation with a mannan oligosaccharide (MOS) on the broiler cecal bacterial community structure and function in a commercial production setting.
25769823	0	19	theme	mannan	107:112	arg1	supplementation					130:144	mannan oligosaccharide supplementation	107:144	mannan oligosaccharide supplementation	107:144	Phylogenetic and functional alterations in bacterial community compositions in broiler ceca as a result of mannan oligosaccharide supplementation.
25769823	1	20	from	structure	306:314	arg1	setting					356:362	a commercial production setting	332:362	a commercial production setting	332:362	This study focused on identifying reproducible effects of dietary supplementation with a mannan oligosaccharide (MOS) on the broiler cecal bacterial community structure and function in a commercial production setting.
25769823	12	21	theme	bacterial	1792:1800	arg1	communities					1802:1812	bacterial communities	1792:1812	bacterial communities as a result of MOS	1792:1831	This indicated that alterations of bacterial communities as a result of MOS are likely to alter the functional capability of the cecum.
25769823	4	22	theme	caught	751:756	arg1	broilers					758:765	12 randomly caught broilers	739:765	12 randomly caught broilers from each group	739:781	Cecal contents were obtained on days 7, 21, and 35 posthatch from 12 randomly caught broilers from each group.
25769823	1	23	theme	reproducible	181:192	arg1	effects					194:200	reproducible effects	181:200	reproducible effects of dietary supplementation with a mannan oligosaccharide (MOS) on the broiler cecal bacterial community structure and function in a commercial production setting	181:362	This study focused on identifying reproducible effects of dietary supplementation with a mannan oligosaccharide (MOS) on the broiler cecal bacterial community structure and function in a commercial production setting.
25769823	6	24	from	points	966:971	arg1	trials					981:986	both trials	976:986	both trials	976:986	The predominant phyla identified at all three time points in both trials were Firmicutes, Bacteroidetes, Proteobacteria, Actinobacteria, and Tenericutes, representing >99% of all sequences.
25769823	11	25	theme	KEGG	1583:1586	arg1	predictions					1606:1616	level 3 KEGG ortholog function predictions	1575:1616	level 3 KEGG ortholog function predictions	1575:1616	Using level 3 KEGG ortholog function predictions, differences between control and supplemented groups were observed, with very strong segregation noted on day 35 posthatch in both trials.
25769823	3	26	theme	same	539:542	arg1	hatchery					555:562	the same commercial hatchery	535:562	the same commercial hatchery	535:562	Approximately 10,000 birds from the same commercial hatchery were mirror imaged into each of four commercial broiler sheds and fed either a control or supplemented diet.
25769823	7	27	theme	days	1176:1179	arg1	supplementation					1181:1195	7 days supplementation	1174:1195	7 days supplementation	1174:1195	MOS supplementation altered the bacterial community composition from 7 days supplementation through 35 days supplementation.
25769823	11	28	from	trials	1749:1754	arg1	posthatch					1731:1739	posthatch	1731:1739	posthatch	1731:1739	Using level 3 KEGG ortholog function predictions, differences between control and supplemented groups were observed, with very strong segregation noted on day 35 posthatch in both trials.
25769823	6	29	theme	time	961:964	arg1	points					966:971	all three time points	951:971	all three time points in both trials	951:986	The predominant phyla identified at all three time points in both trials were Firmicutes, Bacteroidetes, Proteobacteria, Actinobacteria, and Tenericutes, representing >99% of all sequences.
25769823	11	30	theme	supplemented	1651:1662	arg1	groups					1664:1669	supplemented groups	1651:1669	supplemented groups	1651:1669	Using level 3 KEGG ortholog function predictions, differences between control and supplemented groups were observed, with very strong segregation noted on day 35 posthatch in both trials.
25769823	4	31	theme	Cecal	673:677	arg1	contents					679:686	Cecal contents	673:686	Cecal contents	673:686	Cecal contents were obtained on days 7, 21, and 35 posthatch from 12 randomly caught broilers from each group.
25769823	0	32	theme	Phylogenetic	0:11	arg1	alterations					28:38	Phylogenetic and functional alterations	0:38	Phylogenetic and functional alterations in bacterial community compositions in broiler ceca	0:90	Phylogenetic and functional alterations in bacterial community compositions in broiler ceca as a result of mannan oligosaccharide supplementation.
25769823	1	33	theme	bacterial	286:294	arg1	structure					306:314	broiler cecal bacterial community structure	272:314	broiler cecal bacterial community structure	272:314	This study focused on identifying reproducible effects of dietary supplementation with a mannan oligosaccharide (MOS) on the broiler cecal bacterial community structure and function in a commercial production setting.
25769823	11	34	theme	level	1575:1579	arg1	predictions					1606:1616	level 3 KEGG ortholog function predictions	1575:1616	level 3 KEGG ortholog function predictions	1575:1616	Using level 3 KEGG ortholog function predictions, differences between control and supplemented groups were observed, with very strong segregation noted on day 35 posthatch in both trials.
25769823	5	35	theme	Bacterial	784:792	arg1	pyrosequencing					794:807	Bacterial pyrosequencing	784:807	Bacterial pyrosequencing	784:807	Bacterial pyrosequencing was performed on all samples, with approximately 250,000 sequences obtained per treatment per time point.
25769823	1	36	theme	dietary	205:211	arg1	supplementation					213:227	dietary supplementation	205:227	dietary supplementation with a mannan oligosaccharide (MOS)	205:263	This study focused on identifying reproducible effects of dietary supplementation with a mannan oligosaccharide (MOS) on the broiler cecal bacterial community structure and function in a commercial production setting.
25769823	7	37	theme	community	1147:1155	arg1	composition					1157:1167	the bacterial community composition	1133:1167	the bacterial community composition from 7 days supplementation	1133:1195	MOS supplementation altered the bacterial community composition from 7 days supplementation through 35 days supplementation.
25769823	1	38	theme	community	296:304	arg1	structure					306:314	broiler cecal bacterial community structure	272:314	broiler cecal bacterial community structure	272:314	This study focused on identifying reproducible effects of dietary supplementation with a mannan oligosaccharide (MOS) on the broiler cecal bacterial community structure and function in a commercial production setting.
25769823	0	39	theme	supplementation	130:144	arg1	result					97:102	a result	95:102	a result of mannan oligosaccharide supplementation	95:144	Phylogenetic and functional alterations in bacterial community compositions in broiler ceca as a result of mannan oligosaccharide supplementation.
25769823	4	40	theme	posthatch	724:732	arg1	days					705:708	days 7, 21, and 35	705:722	days 7, 21, and 35 posthatch from 12 randomly caught broilers from each group	705:781	Cecal contents were obtained on days 7, 21, and 35 posthatch from 12 randomly caught broilers from each group.
25769823	9	41	theme	supplementation	1375:1389	arg1	effects					1364:1370	The effects	1360:1370	The effects of supplementation	1360:1389	The effects of supplementation were reproducible across both trials.
25769823	9	41	theme	supplementation	1375:1389	arg1	reproducible					1396:1407	reproducible	1396:1407	reproducible	1396:1407	The effects of supplementation were reproducible across both trials.
25769823	1	42	theme	supplementation	213:227	arg1	effects					194:200	reproducible effects	181:200	reproducible effects of dietary supplementation with a mannan oligosaccharide (MOS) on the broiler cecal bacterial community structure and function in a commercial production setting	181:362	This study focused on identifying reproducible effects of dietary supplementation with a mannan oligosaccharide (MOS) on the broiler cecal bacterial community structure and function in a commercial production setting.
25769823	0	43	theme	functional	17:26	arg1	alterations					28:38	Phylogenetic and functional alterations	0:38	Phylogenetic and functional alterations in bacterial community compositions in broiler ceca	0:90	Phylogenetic and functional alterations in bacterial community compositions in broiler ceca as a result of mannan oligosaccharide supplementation.
25769823	0	44	theme	oligosaccharide	114:128	arg1	supplementation					130:144	mannan oligosaccharide supplementation	107:144	mannan oligosaccharide supplementation	107:144	Phylogenetic and functional alterations in bacterial community compositions in broiler ceca as a result of mannan oligosaccharide supplementation.
25769823	8	45	theme	noticeable	1326:1335	arg1	effects					1337:1343	the most noticeable effects	1317:1343	the most noticeable effects after 35 days	1317:1357	Bacteroidetes appeared to be replacing Firmicutes as a result of supplementation, with the most noticeable effects after 35 days.
25769823	11	46	theme	posthatch	1731:1739	arg1	day					1724:1726	day 35 posthatch in both trials	1724:1754	day 35 posthatch in both trials	1724:1754	Using level 3 KEGG ortholog function predictions, differences between control and supplemented groups were observed, with very strong segregation noted on day 35 posthatch in both trials.
25769823	0	47	theme	bacterial	43:51	arg1	compositions					63:74	bacterial community compositions	43:74	bacterial community compositions	43:74	Phylogenetic and functional alterations in bacterial community compositions in broiler ceca as a result of mannan oligosaccharide supplementation.
25769823	1	48	with	supplementation	213:227	arg1	MOS					260:262	MOS	260:262	MOS	260:262	This study focused on identifying reproducible effects of dietary supplementation with a mannan oligosaccharide (MOS) on the broiler cecal bacterial community structure and function in a commercial production setting.
25769823	1	48	with	supplementation	213:227	arg1	oligosaccharide					243:257	a mannan oligosaccharide	234:257	a mannan oligosaccharide (MOS)	234:263	This study focused on identifying reproducible effects of dietary supplementation with a mannan oligosaccharide (MOS) on the broiler cecal bacterial community structure and function in a commercial production setting.
25769823	7	49	from	supplementation	1181:1195	arg1	composition					1157:1167	the bacterial community composition	1133:1167	the bacterial community composition from 7 days supplementation	1133:1195	MOS supplementation altered the bacterial community composition from 7 days supplementation through 35 days supplementation.
25769823	2	50	theme	supplemented	412:423	arg1	group					425:429	a supplemented group	410:429	a supplemented group	410:429	Two separate trials, each with a control and a supplemented group, were carried out in the same commercial location and run concurrently.
25769823	2	51	theme	commercial	461:470	arg1	location					472:479	the same commercial location	452:479	the same commercial location	452:479	Two separate trials, each with a control and a supplemented group, were carried out in the same commercial location and run concurrently.
25769823	7	52	theme	bacterial	1137:1145	arg1	composition					1157:1167	the bacterial community composition	1133:1167	the bacterial community composition from 7 days supplementation	1133:1195	MOS supplementation altered the bacterial community composition from 7 days supplementation through 35 days supplementation.
25769823	1	53	theme	mannan	236:241	arg1	MOS					260:262	MOS	260:262	MOS	260:262	This study focused on identifying reproducible effects of dietary supplementation with a mannan oligosaccharide (MOS) on the broiler cecal bacterial community structure and function in a commercial production setting.
25769823	1	53	theme	mannan	236:241	arg1	oligosaccharide					243:257	a mannan oligosaccharide	234:257	a mannan oligosaccharide (MOS)	234:263	This study focused on identifying reproducible effects of dietary supplementation with a mannan oligosaccharide (MOS) on the broiler cecal bacterial community structure and function in a commercial production setting.
25769823	2	54	theme	same	456:459	arg1	location					472:479	the same commercial location	452:479	the same commercial location	452:479	Two separate trials, each with a control and a supplemented group, were carried out in the same commercial location and run concurrently.
25769823	10	55	theme	communities	1521:1531	arg1	potentials					1493:1502	the functional potentials	1478:1502	the functional potentials of the bacterial communities	1478:1531	PICRUSt was used to identify differences between the functional potentials of the bacterial communities as a result of MOS supplementation.
25769823	11	56	from	posthatch	1731:1739	arg1	trials					1749:1754	both trials	1744:1754	both trials	1744:1754	Using level 3 KEGG ortholog function predictions, differences between control and supplemented groups were observed, with very strong segregation noted on day 35 posthatch in both trials.
25769823	8	57	theme	supplementation	1295:1309	arg1	Firmicutes					1269:1278	Firmicutes	1269:1278	Firmicutes	1269:1278	Bacteroidetes appeared to be replacing Firmicutes as a result of supplementation, with the most noticeable effects after 35 days.
25769823	8	57	theme	supplementation	1295:1309	arg1	result					1285:1290	a result	1283:1290	a result of supplementation	1283:1309	Bacteroidetes appeared to be replacing Firmicutes as a result of supplementation, with the most noticeable effects after 35 days.
25769823	0	58	theme	community	53:61	arg1	compositions					63:74	bacterial community compositions	43:74	bacterial community compositions	43:74	Phylogenetic and functional alterations in bacterial community compositions in broiler ceca as a result of mannan oligosaccharide supplementation.
25769823	6	59	theme	predominant	919:929	arg1	phyla					931:935	The predominant phyla	915:935	The predominant phyla identified at all three time points in both trials	915:986	The predominant phyla identified at all three time points in both trials were Firmicutes, Bacteroidetes, Proteobacteria, Actinobacteria, and Tenericutes, representing >99% of all sequences.
25769823	6	59	theme	predominant	919:929	arg1	Firmicutes					993:1002	Firmicutes	993:1002	Firmicutes	993:1002	The predominant phyla identified at all three time points in both trials were Firmicutes, Bacteroidetes, Proteobacteria, Actinobacteria, and Tenericutes, representing >99% of all sequences.
25769823	0	60	from	alterations	28:38	arg1	ceca					87:90	broiler ceca	79:90	broiler ceca	79:90	Phylogenetic and functional alterations in bacterial community compositions in broiler ceca as a result of mannan oligosaccharide supplementation.
25769823	0	60	from	alterations	28:38	arg1	compositions					63:74	bacterial community compositions	43:74	bacterial community compositions	43:74	Phylogenetic and functional alterations in bacterial community compositions in broiler ceca as a result of mannan oligosaccharide supplementation.
25769823	10	61	theme	functional	1482:1491	arg1	potentials					1493:1502	the functional potentials	1478:1502	the functional potentials of the bacterial communities	1478:1531	PICRUSt was used to identify differences between the functional potentials of the bacterial communities as a result of MOS supplementation.
25769823	11	62	theme	function	1597:1604	arg1	predictions					1606:1616	level 3 KEGG ortholog function predictions	1575:1616	level 3 KEGG ortholog function predictions	1575:1616	Using level 3 KEGG ortholog function predictions, differences between control and supplemented groups were observed, with very strong segregation noted on day 35 posthatch in both trials.
25769823	5	63	theme	time	903:906	arg1	point					908:912	time point	903:912	time point	903:912	Bacterial pyrosequencing was performed on all samples, with approximately 250,000 sequences obtained per treatment per time point.
25769823	1	64	theme	commercial	334:343	arg1	setting					356:362	a commercial production setting	332:362	a commercial production setting	332:362	This study focused on identifying reproducible effects of dietary supplementation with a mannan oligosaccharide (MOS) on the broiler cecal bacterial community structure and function in a commercial production setting.
25769823	0	65	dep	alterations	28:38	arg1	result					97:102	a result	95:102	a result of mannan oligosaccharide supplementation	95:144	Phylogenetic and functional alterations in bacterial community compositions in broiler ceca as a result of mannan oligosaccharide supplementation.
25769823	6	66	theme	sequences	1094:1102	arg1	%					1085:1085	>99%	1082:1085	>99% of all sequences	1082:1102	The predominant phyla identified at all three time points in both trials were Firmicutes, Bacteroidetes, Proteobacteria, Actinobacteria, and Tenericutes, representing >99% of all sequences.
25769823	6	66	theme	sequences	1094:1102	arg1	sequences					1094:1102	all sequences	1090:1102	all sequences	1090:1102	The predominant phyla identified at all three time points in both trials were Firmicutes, Bacteroidetes, Proteobacteria, Actinobacteria, and Tenericutes, representing >99% of all sequences.
25769823	12	67	theme	functional	1857:1866	arg1	capability					1868:1877	the functional capability	1853:1877	the functional capability of the cecum	1853:1890	This indicated that alterations of bacterial communities as a result of MOS are likely to alter the functional capability of the cecum.
25769823	3	68	theme	supplemented	654:665	arg1	diet					667:670	supplemented diet	654:670	supplemented diet	654:670	Approximately 10,000 birds from the same commercial hatchery were mirror imaged into each of four commercial broiler sheds and fed either a control or supplemented diet.
25769823	4	69	from	group	777:781	arg1	broilers					758:765	12 randomly caught broilers	739:765	12 randomly caught broilers from each group	739:781	Cecal contents were obtained on days 7, 21, and 35 posthatch from 12 randomly caught broilers from each group.
25769823	0	70	theme	broiler	79:85	arg1	ceca					87:90	broiler ceca	79:90	broiler ceca	79:90	Phylogenetic and functional alterations in bacterial community compositions in broiler ceca as a result of mannan oligosaccharide supplementation.
25769823	3	71	dep	broiler	612:618	arg1	sheds					620:624	sheds	620:624	sheds	620:624	Approximately 10,000 birds from the same commercial hatchery were mirror imaged into each of four commercial broiler sheds and fed either a control or supplemented diet.
25769823	8	72	theme	most	1321:1324	arg1	effects					1337:1343	the most noticeable effects	1317:1343	the most noticeable effects after 35 days	1317:1357	Bacteroidetes appeared to be replacing Firmicutes as a result of supplementation, with the most noticeable effects after 35 days.
25769823	4	73	from	broilers	758:765	arg1	posthatch					724:732	posthatch	724:732	posthatch	724:732	Cecal contents were obtained on days 7, 21, and 35 posthatch from 12 randomly caught broilers from each group.
25769823	1	74	theme	production	345:354	arg1	setting					356:362	a commercial production setting	332:362	a commercial production setting	332:362	This study focused on identifying reproducible effects of dietary supplementation with a mannan oligosaccharide (MOS) on the broiler cecal bacterial community structure and function in a commercial production setting.
25769823	3	75	dep	mirror	569:574	arg1	imaged					576:581	imaged	576:581	mirror imaged into each of four commercial broiler sheds	569:624	Approximately 10,000 birds from the same commercial hatchery were mirror imaged into each of four commercial broiler sheds and fed either a control or supplemented diet.
27083833	4	0	theme	magnetic	550:557	arg1	resonance					559:567	nuclear magnetic resonance	542:567	nuclear magnetic resonance	542:567	Based on FT-IR, periodate oxidation, Smith degradation, composition analysis and nuclear magnetic resonance experiments, the structure of sanxan polysaccharide was elucidated as follows: The solution of sanxan polysaccharide showed properties of high viscosity and shear-thinning.
27083833	1	1	theme	extracellular	174:186	arg1	biopolymer					188:197	extracellular biopolymer	174:197	extracellular biopolymer termed sanxan	174:211	Sphingomonas sanxanigenens, a new species of the genus Sphingomonas, synthesizes extracellular biopolymer termed sanxan.
27083833	3	2	theme	size-exclusion	393:406	arg1	chromatography					408:421	size-exclusion chromatography	393:421	size-exclusion chromatography combined with laser light scattering	393:458	The molecular weight of sanxan polysaccharide was 408kDa by the method of size-exclusion chromatography combined with laser light scattering.
27083833	3	3	theme	light	443:447	arg1	scattering					449:458	laser light scattering	437:458	laser light scattering	437:458	The molecular weight of sanxan polysaccharide was 408kDa by the method of size-exclusion chromatography combined with laser light scattering.
27083833	3	4	theme	chromatography	408:421	arg1	method					383:388	the method	379:388	the method of size-exclusion chromatography combined with laser light scattering	379:458	The molecular weight of sanxan polysaccharide was 408kDa by the method of size-exclusion chromatography combined with laser light scattering.
27083833	4	5	theme	analysis	529:536	arg1	experiments					569:579	FT-IR, periodate oxidation, Smith degradation, composition analysis and nuclear magnetic resonance experiments	470:579	FT-IR, periodate oxidation, Smith degradation, composition analysis and nuclear magnetic resonance experiments	470:579	Based on FT-IR, periodate oxidation, Smith degradation, composition analysis and nuclear magnetic resonance experiments, the structure of sanxan polysaccharide was elucidated as follows: The solution of sanxan polysaccharide showed properties of high viscosity and shear-thinning.
27083833	4	6	theme	resonance	559:567	arg1	experiments					569:579	FT-IR, periodate oxidation, Smith degradation, composition analysis and nuclear magnetic resonance experiments	470:579	FT-IR, periodate oxidation, Smith degradation, composition analysis and nuclear magnetic resonance experiments	470:579	Based on FT-IR, periodate oxidation, Smith degradation, composition analysis and nuclear magnetic resonance experiments, the structure of sanxan polysaccharide was elucidated as follows: The solution of sanxan polysaccharide showed properties of high viscosity and shear-thinning.
27083833	4	7	theme	shear-thinning	726:739	arg1	properties					693:702	properties	693:702	properties of high viscosity and shear-thinning	693:739	Based on FT-IR, periodate oxidation, Smith degradation, composition analysis and nuclear magnetic resonance experiments, the structure of sanxan polysaccharide was elucidated as follows: The solution of sanxan polysaccharide showed properties of high viscosity and shear-thinning.
27083833	4	8	theme	nuclear	542:548	arg1	resonance					559:567	nuclear magnetic resonance	542:567	nuclear magnetic resonance	542:567	Based on FT-IR, periodate oxidation, Smith degradation, composition analysis and nuclear magnetic resonance experiments, the structure of sanxan polysaccharide was elucidated as follows: The solution of sanxan polysaccharide showed properties of high viscosity and shear-thinning.
27083833	1	9	theme	Sphingomonas	93:104	arg1	species					127:133	a new species	121:133	a new species of the genus Sphingomonas	121:159	Sphingomonas sanxanigenens, a new species of the genus Sphingomonas, synthesizes extracellular biopolymer termed sanxan.
27083833	1	9	theme	Sphingomonas	93:104	arg1	sanxanigenens					106:118	Sphingomonas sanxanigenens	93:118	Sphingomonas sanxanigenens	93:118	Sphingomonas sanxanigenens, a new species of the genus Sphingomonas, synthesizes extracellular biopolymer termed sanxan.
27083833	3	10	theme	laser	437:441	arg1	scattering					449:458	laser light scattering	437:458	laser light scattering	437:458	The molecular weight of sanxan polysaccharide was 408kDa by the method of size-exclusion chromatography combined with laser light scattering.
27083833	4	11	theme	composition	517:527	arg1	analysis					529:536	composition analysis	517:536	composition analysis	517:536	Based on FT-IR, periodate oxidation, Smith degradation, composition analysis and nuclear magnetic resonance experiments, the structure of sanxan polysaccharide was elucidated as follows: The solution of sanxan polysaccharide showed properties of high viscosity and shear-thinning.
27083833	0	12	theme	Structural	0:9	arg1	properties					24:33	Structural and physical properties	0:33	Structural and physical properties of sanxan polysaccharide from Sphingomonas sanxanigenens	0:90	Structural and physical properties of sanxan polysaccharide from Sphingomonas sanxanigenens.
27083833	4	13	theme	periodate	477:485	arg1	oxidation					487:495	periodate oxidation	477:495	periodate oxidation	477:495	Based on FT-IR, periodate oxidation, Smith degradation, composition analysis and nuclear magnetic resonance experiments, the structure of sanxan polysaccharide was elucidated as follows: The solution of sanxan polysaccharide showed properties of high viscosity and shear-thinning.
27083833	4	14	theme	polysaccharide	606:619	arg1	follows					639:645	follows	639:645	follows	639:645	Based on FT-IR, periodate oxidation, Smith degradation, composition analysis and nuclear magnetic resonance experiments, the structure of sanxan polysaccharide was elucidated as follows: The solution of sanxan polysaccharide showed properties of high viscosity and shear-thinning.
27083833	4	14	theme	polysaccharide	606:619	arg1	structure					586:594	the structure	582:594	the structure of sanxan polysaccharide	582:619	Based on FT-IR, periodate oxidation, Smith degradation, composition analysis and nuclear magnetic resonance experiments, the structure of sanxan polysaccharide was elucidated as follows: The solution of sanxan polysaccharide showed properties of high viscosity and shear-thinning.
27083833	5	15	theme	sanxan	768:773	arg1	polysaccharide					775:788	sanxan polysaccharide	768:788	sanxan polysaccharide	768:788	By cooling hot solutions, sanxan polysaccharide could form elastic thermoreversible gel.
27083833	5	16	theme	thermoreversible	809:824	arg1	gel					826:828	elastic thermoreversible gel	801:828	elastic thermoreversible gel	801:828	By cooling hot solutions, sanxan polysaccharide could form elastic thermoreversible gel.
27083833	0	17	theme	physical	15:22	arg1	properties					24:33	Structural and physical properties	0:33	Structural and physical properties of sanxan polysaccharide from Sphingomonas sanxanigenens	0:90	Structural and physical properties of sanxan polysaccharide from Sphingomonas sanxanigenens.
27083833	3	18	theme	molecular	323:331	arg1	weight					333:338	The molecular weight	319:338	The molecular weight of sanxan polysaccharide	319:363	The molecular weight of sanxan polysaccharide was 408kDa by the method of size-exclusion chromatography combined with laser light scattering.
27083833	4	19	theme	Smith	498:502	arg1	degradation					504:514	Smith degradation	498:514	Smith degradation	498:514	Based on FT-IR, periodate oxidation, Smith degradation, composition analysis and nuclear magnetic resonance experiments, the structure of sanxan polysaccharide was elucidated as follows: The solution of sanxan polysaccharide showed properties of high viscosity and shear-thinning.
27083833	1	20	theme	new	123:125	arg1	species					127:133	a new species	121:133	a new species of the genus Sphingomonas	121:159	Sphingomonas sanxanigenens, a new species of the genus Sphingomonas, synthesizes extracellular biopolymer termed sanxan.
27083833	1	20	theme	new	123:125	arg1	sanxanigenens					106:118	Sphingomonas sanxanigenens	93:118	Sphingomonas sanxanigenens	93:118	Sphingomonas sanxanigenens, a new species of the genus Sphingomonas, synthesizes extracellular biopolymer termed sanxan.
27083833	0	21	theme	sanxan	38:43	arg1	polysaccharide					45:58	sanxan polysaccharide	38:58	sanxan polysaccharide from Sphingomonas sanxanigenens	38:90	Structural and physical properties of sanxan polysaccharide from Sphingomonas sanxanigenens.
27083833	4	22	theme	viscosity	712:720	arg1	properties					693:702	properties	693:702	properties of high viscosity and shear-thinning	693:739	Based on FT-IR, periodate oxidation, Smith degradation, composition analysis and nuclear magnetic resonance experiments, the structure of sanxan polysaccharide was elucidated as follows: The solution of sanxan polysaccharide showed properties of high viscosity and shear-thinning.
27083833	0	23	from	sanxanigenens	78:90	arg1	properties					24:33	Structural and physical properties	0:33	Structural and physical properties of sanxan polysaccharide from Sphingomonas sanxanigenens	0:90	Structural and physical properties of sanxan polysaccharide from Sphingomonas sanxanigenens.
27083833	0	23	from	sanxanigenens	78:90	arg1	polysaccharide					45:58	sanxan polysaccharide	38:58	sanxan polysaccharide from Sphingomonas sanxanigenens	38:90	Structural and physical properties of sanxan polysaccharide from Sphingomonas sanxanigenens.
27083833	2	24	theme	fermentation	258:269	arg1	broth					271:275	the fermentation broth	254:275	the fermentation broth	254:275	Sanxan polysaccharide was purified from the fermentation broth by Sephacryl S-400 column chromatography.
27083833	4	25	theme	high	707:710	arg1	viscosity					712:720	high viscosity	707:720	high viscosity	707:720	Based on FT-IR, periodate oxidation, Smith degradation, composition analysis and nuclear magnetic resonance experiments, the structure of sanxan polysaccharide was elucidated as follows: The solution of sanxan polysaccharide showed properties of high viscosity and shear-thinning.
27083833	5	26	theme	elastic	801:807	arg1	gel					826:828	elastic thermoreversible gel	801:828	elastic thermoreversible gel	801:828	By cooling hot solutions, sanxan polysaccharide could form elastic thermoreversible gel.
27083833	3	27	theme	sanxan	343:348	arg1	polysaccharide					350:363	sanxan polysaccharide	343:363	sanxan polysaccharide	343:363	The molecular weight of sanxan polysaccharide was 408kDa by the method of size-exclusion chromatography combined with laser light scattering.
27083833	4	28	theme	degradation	504:514	arg1	experiments					569:579	FT-IR, periodate oxidation, Smith degradation, composition analysis and nuclear magnetic resonance experiments	470:579	FT-IR, periodate oxidation, Smith degradation, composition analysis and nuclear magnetic resonance experiments	470:579	Based on FT-IR, periodate oxidation, Smith degradation, composition analysis and nuclear magnetic resonance experiments, the structure of sanxan polysaccharide was elucidated as follows: The solution of sanxan polysaccharide showed properties of high viscosity and shear-thinning.
27083833	0	29	theme	polysaccharide	45:58	arg1	properties					24:33	Structural and physical properties	0:33	Structural and physical properties of sanxan polysaccharide from Sphingomonas sanxanigenens	0:90	Structural and physical properties of sanxan polysaccharide from Sphingomonas sanxanigenens.
27083833	4	30	theme	sanxan	599:604	arg1	polysaccharide					606:619	sanxan polysaccharide	599:619	sanxan polysaccharide	599:619	Based on FT-IR, periodate oxidation, Smith degradation, composition analysis and nuclear magnetic resonance experiments, the structure of sanxan polysaccharide was elucidated as follows: The solution of sanxan polysaccharide showed properties of high viscosity and shear-thinning.
27083833	3	31	theme	polysaccharide	350:363	arg1	weight					333:338	The molecular weight	319:338	The molecular weight of sanxan polysaccharide	319:363	The molecular weight of sanxan polysaccharide was 408kDa by the method of size-exclusion chromatography combined with laser light scattering.
27083833	4	32	theme	FT-IR	470:474	arg1	experiments					569:579	FT-IR, periodate oxidation, Smith degradation, composition analysis and nuclear magnetic resonance experiments	470:579	FT-IR, periodate oxidation, Smith degradation, composition analysis and nuclear magnetic resonance experiments	470:579	Based on FT-IR, periodate oxidation, Smith degradation, composition analysis and nuclear magnetic resonance experiments, the structure of sanxan polysaccharide was elucidated as follows: The solution of sanxan polysaccharide showed properties of high viscosity and shear-thinning.
27083833	2	33	theme	column	296:301	arg1	chromatography					303:316	Sephacryl S-400 column chromatography	280:316	Sephacryl S-400 column chromatography	280:316	Sanxan polysaccharide was purified from the fermentation broth by Sephacryl S-400 column chromatography.
27083833	4	34	theme	sanxan	664:669	arg1	polysaccharide					671:684	sanxan polysaccharide	664:684	sanxan polysaccharide	664:684	Based on FT-IR, periodate oxidation, Smith degradation, composition analysis and nuclear magnetic resonance experiments, the structure of sanxan polysaccharide was elucidated as follows: The solution of sanxan polysaccharide showed properties of high viscosity and shear-thinning.
27083833	4	35	theme	oxidation	487:495	arg1	experiments					569:579	FT-IR, periodate oxidation, Smith degradation, composition analysis and nuclear magnetic resonance experiments	470:579	FT-IR, periodate oxidation, Smith degradation, composition analysis and nuclear magnetic resonance experiments	470:579	Based on FT-IR, periodate oxidation, Smith degradation, composition analysis and nuclear magnetic resonance experiments, the structure of sanxan polysaccharide was elucidated as follows: The solution of sanxan polysaccharide showed properties of high viscosity and shear-thinning.
27083833	2	36	theme	S-400	290:294	arg1	chromatography					303:316	Sephacryl S-400 column chromatography	280:316	Sephacryl S-400 column chromatography	280:316	Sanxan polysaccharide was purified from the fermentation broth by Sephacryl S-400 column chromatography.
27083833	1	37	theme	genus	142:146	arg1	Sphingomonas					148:159	the genus Sphingomonas	138:159	the genus Sphingomonas	138:159	Sphingomonas sanxanigenens, a new species of the genus Sphingomonas, synthesizes extracellular biopolymer termed sanxan.
27083833	2	38	theme	Sanxan	214:219	arg1	polysaccharide					221:234	Sanxan polysaccharide	214:234	Sanxan polysaccharide	214:234	Sanxan polysaccharide was purified from the fermentation broth by Sephacryl S-400 column chromatography.
27083833	2	39	theme	Sephacryl	280:288	arg1	chromatography					303:316	Sephacryl S-400 column chromatography	280:316	Sephacryl S-400 column chromatography	280:316	Sanxan polysaccharide was purified from the fermentation broth by Sephacryl S-400 column chromatography.
27083833	1	40	theme	Sphingomonas	148:159	arg1	species					127:133	a new species	121:133	a new species of the genus Sphingomonas	121:159	Sphingomonas sanxanigenens, a new species of the genus Sphingomonas, synthesizes extracellular biopolymer termed sanxan.
27083833	1	40	theme	Sphingomonas	148:159	arg1	sanxanigenens					106:118	Sphingomonas sanxanigenens	93:118	Sphingomonas sanxanigenens	93:118	Sphingomonas sanxanigenens, a new species of the genus Sphingomonas, synthesizes extracellular biopolymer termed sanxan.
27083833	0	41	theme	Sphingomonas	65:76	arg1	sanxanigenens					78:90	Sphingomonas sanxanigenens	65:90	Sphingomonas sanxanigenens	65:90	Structural and physical properties of sanxan polysaccharide from Sphingomonas sanxanigenens.
27083833	0	42	from	properties	24:33	arg1	sanxanigenens					78:90	Sphingomonas sanxanigenens	65:90	Sphingomonas sanxanigenens	65:90	Structural and physical properties of sanxan polysaccharide from Sphingomonas sanxanigenens.
27083833	5	43	theme	hot	753:755	arg1	solutions					757:765	hot solutions	753:765	hot solutions	753:765	By cooling hot solutions, sanxan polysaccharide could form elastic thermoreversible gel.
27083833	4	44	theme	polysaccharide	671:684	arg1	solution					652:659	The solution	648:659	The solution of sanxan polysaccharide	648:684	Based on FT-IR, periodate oxidation, Smith degradation, composition analysis and nuclear magnetic resonance experiments, the structure of sanxan polysaccharide was elucidated as follows: The solution of sanxan polysaccharide showed properties of high viscosity and shear-thinning.
29579900	9	0	theme	systems	1263:1269	arg1	size					1216:1219	Fat droplet size	1204:1219	Fat droplet size of the emulsion and gelled emulsion model systems	1204:1269	Fat droplet size of the emulsion and gelled emulsion model systems was evenly distributed around 1μm.
29579900	10	1	theme	aspects	1405:1411	arg1	influence					1382:1390	the influence	1378:1390	the influence of important aspects of food microstructure on microbial dynamics	1378:1456	In general, the set of model systems was proven to be suitable to study the influence of important aspects of food microstructure on microbial dynamics.
29579900	5	2	theme	mild	724:727	arg1	experiments					750:760	mild thermal inactivation experiments	724:760	mild thermal inactivation experiments	724:760	The systems were suitable for common growth and mild thermal inactivation experiments involving both homogeneous and surface inoculation.
29579900	8	3	theme	1.25±0.05N	1178:1187	arg1	hardness					1151:1158	a hardness	1149:1158	a hardness of 1.35±0.07N and 1.25±0.05N	1149:1187	Both the aqueous gel and gelled emulsion were classified as strong gels, with a hardness of 1.35±0.07N and 1.25±0.05N, respectively.
29579900	10	4	theme	important	1395:1403	arg1	aspects					1405:1411	important aspects	1395:1411	important aspects of food microstructure	1395:1434	In general, the set of model systems was proven to be suitable to study the influence of important aspects of food microstructure on microbial dynamics.
29579900	8	5	with	gels	1138:1141	arg1	hardness					1151:1158	a hardness	1149:1158	a hardness of 1.35±0.07N and 1.25±0.05N	1149:1187	Both the aqueous gel and gelled emulsion were classified as strong gels, with a hardness of 1.35±0.07N and 1.25±0.05N, respectively.
29579900	9	6	theme	droplet	1208:1214	arg1	size					1216:1219	Fat droplet size	1204:1219	Fat droplet size of the emulsion and gelled emulsion model systems	1204:1269	Fat droplet size of the emulsion and gelled emulsion model systems was evenly distributed around 1μm.
29579900	5	7	theme	common	706:711	arg1	growth					713:718	common growth	706:718	common growth	706:718	The systems were suitable for common growth and mild thermal inactivation experiments involving both homogeneous and surface inoculation.
29579900	1	8	from	influence	166:174	arg1	dynamics					212:219	microbial dynamics	202:219	microbial dynamics	202:219	The effectiveness of predictive microbiology is limited by the lack of knowledge concerning the influence of food microstructure on microbial dynamics.
29579900	2	9	theme	food	304:307	arg1	systems					315:321	structured food model systems	293:321	structured food model systems as well as liquid systems	293:347	Therefore, future modelling attempts should be based on experiments in structured food model systems as well as liquid systems.
29579900	4	10	theme	microstructural	548:562	arg1	effect					564:569	The microstructural effect	544:569	The microstructural effect	544:569	The microstructural effect was isolated by minimising compositional and physico-chemical changes among the different model systems.
29579900	7	11	dep	exhibited	1019:1027	arg1	non-Newtonian					1030:1042	non-Newtonian	1030:1042	non-Newtonian	1030:1042	The liquid system without xanthan gum behaved like a Newtonian fluid, while the emulsion and the liquid containing xanthan gum exhibited (non-Newtonian) pseudo-plastic behaviour.
29579900	2	12	theme	structured	293:302	arg1	systems					315:321	structured food model systems	293:321	structured food model systems as well as liquid systems	293:347	Therefore, future modelling attempts should be based on experiments in structured food model systems as well as liquid systems.
29579900	7	13	theme	Newtonian	945:953	arg1	fluid					955:959	a Newtonian fluid	943:959	a Newtonian fluid	943:959	The liquid system without xanthan gum behaved like a Newtonian fluid, while the emulsion and the liquid containing xanthan gum exhibited (non-Newtonian) pseudo-plastic behaviour.
29579900	4	14	theme	different	651:659	arg1	systems					667:673	the different model systems	647:673	the different model systems	647:673	The microstructural effect was isolated by minimising compositional and physico-chemical changes among the different model systems.
29579900	5	15	theme	surface	793:799	arg1	inoculation					801:811	surface inoculation	793:811	surface inoculation	793:811	The systems were suitable for common growth and mild thermal inactivation experiments involving both homogeneous and surface inoculation.
29579900	10	16	theme	microstructure	1421:1434	arg1	aspects					1405:1411	important aspects	1395:1411	important aspects of food microstructure	1395:1434	In general, the set of model systems was proven to be suitable to study the influence of important aspects of food microstructure on microbial dynamics.
29579900	10	17	theme	microbial	1439:1447	arg1	dynamics					1449:1456	microbial dynamics	1439:1456	microbial dynamics	1439:1456	In general, the set of model systems was proven to be suitable to study the influence of important aspects of food microstructure on microbial dynamics.
29579900	1	18	theme	food	179:182	arg1	microstructure					184:197	food microstructure	179:197	food microstructure	179:197	The effectiveness of predictive microbiology is limited by the lack of knowledge concerning the influence of food microstructure on microbial dynamics.
29579900	8	19	theme	1.35±0.07N	1163:1172	arg1	hardness					1151:1158	a hardness	1149:1158	a hardness of 1.35±0.07N and 1.25±0.05N	1149:1187	Both the aqueous gel and gelled emulsion were classified as strong gels, with a hardness of 1.35±0.07N and 1.25±0.05N, respectively.
29579900	8	20	theme	strong	1131:1136	arg1	gels					1138:1141	strong gels	1131:1141	strong gels	1131:1141	Both the aqueous gel and gelled emulsion were classified as strong gels, with a hardness of 1.35±0.07N and 1.25±0.05N, respectively.
29579900	8	20	theme	strong	1131:1136	arg1	emulsion					1103:1110	gelled emulsion	1096:1110	gelled emulsion	1096:1110	Both the aqueous gel and gelled emulsion were classified as strong gels, with a hardness of 1.35±0.07N and 1.25±0.05N, respectively.
29579900	8	20	theme	strong	1131:1136	arg1	gel					1088:1090	the aqueous gel	1076:1090	the aqueous gel	1076:1090	Both the aqueous gel and gelled emulsion were classified as strong gels, with a hardness of 1.35±0.07N and 1.25±0.05N, respectively.
29579900	10	21	theme	food	1416:1419	arg1	microstructure					1421:1434	food microstructure	1416:1434	food microstructure	1416:1434	In general, the set of model systems was proven to be suitable to study the influence of important aspects of food microstructure on microbial dynamics.
29579900	1	22	theme	predictive	91:100	arg1	microbiology					102:113	predictive microbiology	91:113	predictive microbiology	91:113	The effectiveness of predictive microbiology is limited by the lack of knowledge concerning the influence of food microstructure on microbial dynamics.
29579900	1	23	theme	microstructure	184:197	arg1	influence					166:174	the influence	162:174	the influence of food microstructure on microbial dynamics	162:219	The effectiveness of predictive microbiology is limited by the lack of knowledge concerning the influence of food microstructure on microbial dynamics.
29579900	6	24	theme	Average	814:820	arg1	pH					822:823	Average pH	814:823	Average pH of the model systems	814:844	Average pH of the model systems was 6.36±0.03 and average aw was 0.988±0.002.
29579900	7	25	theme	xanthan	918:924	arg1	gum					926:928	xanthan gum	918:928	xanthan gum	918:928	The liquid system without xanthan gum behaved like a Newtonian fluid, while the emulsion and the liquid containing xanthan gum exhibited (non-Newtonian) pseudo-plastic behaviour.
29579900	1	26	theme	microbiology	102:113	arg1	effectiveness					74:86	The effectiveness	70:86	The effectiveness of predictive microbiology	70:113	The effectiveness of predictive microbiology is limited by the lack of knowledge concerning the influence of food microstructure on microbial dynamics.
29579900	0	27	theme	model	26:30	arg1	systems					32:38	fish-based model systems	15:38	fish-based model systems	15:38	Development of fish-based model systems with various microstructures.
29579900	10	28	from	influence	1382:1390	arg1	dynamics					1449:1456	microbial dynamics	1439:1456	microbial dynamics	1439:1456	In general, the set of model systems was proven to be suitable to study the influence of important aspects of food microstructure on microbial dynamics.
29579900	9	29	theme	Fat	1204:1206	arg1	size					1216:1219	Fat droplet size	1204:1219	Fat droplet size of the emulsion and gelled emulsion model systems	1204:1269	Fat droplet size of the emulsion and gelled emulsion model systems was evenly distributed around 1μm.
29579900	3	30	with	systems	382:388	arg1	microstructures					403:417	various microstructures	395:417	various microstructures	395:417	In this study, fish-based model systems with various microstructures were developed, i.e., two liquid systems (with and without xanthan gum), an emulsion, an aqueous gel, and a gelled emulsion.
29579900	1	31	theme	microbial	202:210	arg1	dynamics					212:219	microbial dynamics	202:219	microbial dynamics	202:219	The effectiveness of predictive microbiology is limited by the lack of knowledge concerning the influence of food microstructure on microbial dynamics.
29579900	3	32	theme	model	376:380	arg1	systems					382:388	fish-based model systems	365:388	fish-based model systems with various microstructures	365:417	In this study, fish-based model systems with various microstructures were developed, i.e., two liquid systems (with and without xanthan gum), an emulsion, an aqueous gel, and a gelled emulsion.
29579900	6	33	theme	average	864:870	arg1	aw					872:873	average aw	864:873	average aw	864:873	Average pH of the model systems was 6.36±0.03 and average aw was 0.988±0.002.
29579900	2	34	theme	model	309:313	arg1	systems					315:321	structured food model systems	293:321	structured food model systems as well as liquid systems	293:347	Therefore, future modelling attempts should be based on experiments in structured food model systems as well as liquid systems.
29579900	7	35	dep	emulsion	972:979	arg1	the					968:970	the	968:970	the	968:970	The liquid system without xanthan gum behaved like a Newtonian fluid, while the emulsion and the liquid containing xanthan gum exhibited (non-Newtonian) pseudo-plastic behaviour.
29579900	4	36	theme	physico-chemical	616:631	arg1	changes					633:639	compositional and physico-chemical changes	598:639	compositional and physico-chemical changes	598:639	The microstructural effect was isolated by minimising compositional and physico-chemical changes among the different model systems.
29579900	3	37	theme	liquid	445:450	arg1	systems					452:458	two liquid systems	441:458	two liquid systems (with and without xanthan gum)	441:489	In this study, fish-based model systems with various microstructures were developed, i.e., two liquid systems (with and without xanthan gum), an emulsion, an aqueous gel, and a gelled emulsion.
29579900	0	38	theme	systems	32:38	arg1	Development					0:10	Development	0:10	Development of fish-based model systems with various microstructures.	0:68	Development of fish-based model systems with various microstructures.
29579900	7	39	theme	pseudo-plastic	1045:1058	arg1	behaviour					1060:1068	pseudo-plastic behaviour	1045:1068	pseudo-plastic behaviour	1045:1068	The liquid system without xanthan gum behaved like a Newtonian fluid, while the emulsion and the liquid containing xanthan gum exhibited (non-Newtonian) pseudo-plastic behaviour.
29579900	3	40	theme	fish-based	365:374	arg1	systems					382:388	fish-based model systems	365:388	fish-based model systems with various microstructures	365:417	In this study, fish-based model systems with various microstructures were developed, i.e., two liquid systems (with and without xanthan gum), an emulsion, an aqueous gel, and a gelled emulsion.
29579900	2	41	dep	modelling	240:248	arg1	attempts					250:257	attempts	250:257	attempts	250:257	Therefore, future modelling attempts should be based on experiments in structured food model systems as well as liquid systems.
29579900	3	42	theme	various	395:401	arg1	microstructures					403:417	various microstructures	395:417	various microstructures	395:417	In this study, fish-based model systems with various microstructures were developed, i.e., two liquid systems (with and without xanthan gum), an emulsion, an aqueous gel, and a gelled emulsion.
29579900	8	43	theme	gelled	1096:1101	arg1	gels					1138:1141	strong gels	1131:1141	strong gels	1131:1141	Both the aqueous gel and gelled emulsion were classified as strong gels, with a hardness of 1.35±0.07N and 1.25±0.05N, respectively.
29579900	8	43	theme	gelled	1096:1101	arg1	emulsion					1103:1110	gelled emulsion	1096:1110	gelled emulsion	1096:1110	Both the aqueous gel and gelled emulsion were classified as strong gels, with a hardness of 1.35±0.07N and 1.25±0.05N, respectively.
29579900	8	43	theme	gelled	1096:1101	arg1	gel					1088:1090	the aqueous gel	1076:1090	the aqueous gel	1076:1090	Both the aqueous gel and gelled emulsion were classified as strong gels, with a hardness of 1.35±0.07N and 1.25±0.05N, respectively.
29579900	2	44	theme	future	233:238	arg1	modelling					240:248	future modelling attempts	233:257	future modelling attempts	233:257	Therefore, future modelling attempts should be based on experiments in structured food model systems as well as liquid systems.
29579900	7	45	theme	xanthan	1007:1013	arg1	gum					1015:1017	xanthan gum	1007:1017	xanthan gum	1007:1017	The liquid system without xanthan gum behaved like a Newtonian fluid, while the emulsion and the liquid containing xanthan gum exhibited (non-Newtonian) pseudo-plastic behaviour.
29579900	3	46	theme	xanthan	478:484	arg1	gum					486:488	xanthan gum	478:488	xanthan gum	478:488	In this study, fish-based model systems with various microstructures were developed, i.e., two liquid systems (with and without xanthan gum), an emulsion, an aqueous gel, and a gelled emulsion.
29579900	0	47	theme	various	45:51	arg1	microstructures					53:67	various microstructures	45:67	various microstructures	45:67	Development of fish-based model systems with various microstructures.
29579900	9	48	theme	emulsion	1228:1235	arg1	size					1216:1219	Fat droplet size	1204:1219	Fat droplet size of the emulsion and gelled emulsion model systems	1204:1269	Fat droplet size of the emulsion and gelled emulsion model systems was evenly distributed around 1μm.
29579900	6	49	theme	systems	838:844	arg1	pH					822:823	Average pH	814:823	Average pH of the model systems	814:844	Average pH of the model systems was 6.36±0.03 and average aw was 0.988±0.002.
29579900	10	50	theme	model	1329:1333	arg1	systems					1335:1341	model systems	1329:1341	model systems	1329:1341	In general, the set of model systems was proven to be suitable to study the influence of important aspects of food microstructure on microbial dynamics.
29579900	6	51	theme	model	832:836	arg1	systems					838:844	the model systems	828:844	the model systems	828:844	Average pH of the model systems was 6.36±0.03 and average aw was 0.988±0.002.
29579900	0	52	with	Development	0:10	arg1	microstructures					53:67	various microstructures	45:67	various microstructures	45:67	Development of fish-based model systems with various microstructures.
29579900	2	53	theme	liquid	334:339	arg1	systems					341:347	liquid systems	334:347	structured food model systems as well as liquid systems	293:347	Therefore, future modelling attempts should be based on experiments in structured food model systems as well as liquid systems.
29579900	2	54	from	experiments	278:288	arg1	systems					341:347	liquid systems	334:347	structured food model systems as well as liquid systems	293:347	Therefore, future modelling attempts should be based on experiments in structured food model systems as well as liquid systems.
29579900	2	54	from	experiments	278:288	arg1	systems					315:321	structured food model systems	293:321	structured food model systems as well as liquid systems	293:347	Therefore, future modelling attempts should be based on experiments in structured food model systems as well as liquid systems.
29579900	9	55	theme	gelled	1241:1246	arg1	systems					1263:1269	the emulsion and gelled emulsion model systems	1224:1269	systems	1263:1269	Fat droplet size of the emulsion and gelled emulsion model systems was evenly distributed around 1μm.
29579900	3	56	theme	aqueous	508:514	arg1	gel					516:518	an aqueous gel	505:518	an aqueous gel	505:518	In this study, fish-based model systems with various microstructures were developed, i.e., two liquid systems (with and without xanthan gum), an emulsion, an aqueous gel, and a gelled emulsion.
29579900	10	57	theme	systems	1335:1341	arg1	suitable					1360:1367	suitable	1360:1367	suitable	1360:1367	In general, the set of model systems was proven to be suitable to study the influence of important aspects of food microstructure on microbial dynamics.
29579900	10	57	theme	systems	1335:1341	arg1	set					1322:1324	the set	1318:1324	the set of model systems	1318:1341	In general, the set of model systems was proven to be suitable to study the influence of important aspects of food microstructure on microbial dynamics.
29579900	10	57	theme	systems	1335:1341	arg1	systems					1335:1341	model systems	1329:1341	model systems	1329:1341	In general, the set of model systems was proven to be suitable to study the influence of important aspects of food microstructure on microbial dynamics.
29579900	4	58	theme	model	661:665	arg1	systems					667:673	the different model systems	647:673	the different model systems	647:673	The microstructural effect was isolated by minimising compositional and physico-chemical changes among the different model systems.
29579900	7	59	contain	containing	996:1005	arg1	emulsion					972:979	emulsion	972:979	emulsion	972:979	The liquid system without xanthan gum behaved like a Newtonian fluid, while the emulsion and the liquid containing xanthan gum exhibited (non-Newtonian) pseudo-plastic behaviour.
29579900	7	59	contain	containing	996:1005	arg2	gum					1015:1017	xanthan gum	1007:1017	xanthan gum	1007:1017	The liquid system without xanthan gum behaved like a Newtonian fluid, while the emulsion and the liquid containing xanthan gum exhibited (non-Newtonian) pseudo-plastic behaviour.
29579900	5	60	theme	thermal	729:735	arg1	experiments					750:760	mild thermal inactivation experiments	724:760	mild thermal inactivation experiments	724:760	The systems were suitable for common growth and mild thermal inactivation experiments involving both homogeneous and surface inoculation.
29579900	9	61	theme	emulsion	1248:1255	arg1	systems					1263:1269	the emulsion and gelled emulsion model systems	1224:1269	systems	1263:1269	Fat droplet size of the emulsion and gelled emulsion model systems was evenly distributed around 1μm.
29579900	4	62	theme	compositional	598:610	arg1	changes					633:639	compositional and physico-chemical changes	598:639	compositional and physico-chemical changes	598:639	The microstructural effect was isolated by minimising compositional and physico-chemical changes among the different model systems.
29579900	1	63	theme	knowledge	141:149	arg1	lack					133:136	the lack	129:136	the lack of knowledge concerning the influence of food microstructure on microbial dynamics	129:219	The effectiveness of predictive microbiology is limited by the lack of knowledge concerning the influence of food microstructure on microbial dynamics.
29579900	7	64	theme	liquid	896:901	arg1	system					903:908	The liquid system	892:908	The liquid system without xanthan gum	892:928	The liquid system without xanthan gum behaved like a Newtonian fluid, while the emulsion and the liquid containing xanthan gum exhibited (non-Newtonian) pseudo-plastic behaviour.
29579900	5	65	theme	inactivation	737:748	arg1	experiments					750:760	mild thermal inactivation experiments	724:760	mild thermal inactivation experiments	724:760	The systems were suitable for common growth and mild thermal inactivation experiments involving both homogeneous and surface inoculation.
29579900	9	66	theme	model	1257:1261	arg1	systems					1263:1269	the emulsion and gelled emulsion model systems	1224:1269	systems	1263:1269	Fat droplet size of the emulsion and gelled emulsion model systems was evenly distributed around 1μm.
29579900	0	67	theme	fish-based	15:24	arg1	systems					32:38	fish-based model systems	15:38	fish-based model systems	15:38	Development of fish-based model systems with various microstructures.
29579900	3	68	theme	gelled	527:532	arg1	emulsion					534:541	a gelled emulsion	525:541	a gelled emulsion	525:541	In this study, fish-based model systems with various microstructures were developed, i.e., two liquid systems (with and without xanthan gum), an emulsion, an aqueous gel, and a gelled emulsion.
29579900	8	69	theme	aqueous	1080:1086	arg1	gels					1138:1141	strong gels	1131:1141	strong gels	1131:1141	Both the aqueous gel and gelled emulsion were classified as strong gels, with a hardness of 1.35±0.07N and 1.25±0.05N, respectively.
29579900	8	69	theme	aqueous	1080:1086	arg1	gel					1088:1090	the aqueous gel	1076:1090	the aqueous gel	1076:1090	Both the aqueous gel and gelled emulsion were classified as strong gels, with a hardness of 1.35±0.07N and 1.25±0.05N, respectively.
29579900	8	69	theme	aqueous	1080:1086	arg1	emulsion					1103:1110	gelled emulsion	1096:1110	gelled emulsion	1096:1110	Both the aqueous gel and gelled emulsion were classified as strong gels, with a hardness of 1.35±0.07N and 1.25±0.05N, respectively.
25486437	2	0	theme	functional	324:333	arg1	studies					335:341	functional studies	324:341	functional studies other than the two cases mentioned above	324:382	However, the low natural abundance of HS 3-O-sulfation poses a serious challenge for functional studies other than the two cases mentioned above.
25486437	7	1	theme	novel	1102:1106	arg1	reaction					1116:1123	this novel peeling reaction	1097:1123	this novel peeling reaction	1097:1123	Our discovery calls for the re-evaluation of the natural abundance and functions of HS 3-O-sulfation by taking into consideration the negative impact of this novel peeling reaction.
25486437	7	2	theme	natural	993:999	arg1	abundance					1001:1009	natural abundance	993:1009	natural abundance	993:1009	Our discovery calls for the re-evaluation of the natural abundance and functions of HS 3-O-sulfation by taking into consideration the negative impact of this novel peeling reaction.
25486437	2	3	theme	natural	256:262	arg1	abundance					264:272	the low natural abundance	248:272	the low natural abundance of HS 3-O-sulfation	248:292	However, the low natural abundance of HS 3-O-sulfation poses a serious challenge for functional studies other than the two cases mentioned above.
25486437	4	4	theme	HS	561:562	arg1	chains					564:569	HS chains	561:569	HS chains with 3-O-sulfated glucosamine	561:599	Here we describe a novel peeling reaction that specifically degrades HS chains with 3-O-sulfated glucosamine at the reducing-end.
25486437	5	5	theme	reducing	729:736	arg1	ends					738:741	the reducing ends	725:741	the reducing ends	725:741	When HS/heparin is enzymatically depolymerized for compositional analysis, 3-O-sulfated glucosamine at the reducing ends appears to be susceptible to degradation under mildly basic conditions.
25486437	6	6	theme	side-reaction	911:923	arg1	products					925:932	the intermediate and side-reaction products	890:932	the intermediate and side-reaction products observed	890:941	We propose a 3-O-desulfation initiated peeling reaction mechanism based on the intermediate and side-reaction products observed.
25486437	2	7	theme	low	252:254	arg1	abundance					264:272	the low natural abundance	248:272	the low natural abundance of HS 3-O-sulfation	248:292	However, the low natural abundance of HS 3-O-sulfation poses a serious challenge for functional studies other than the two cases mentioned above.
25486437	1	8	theme	Heparan	72:78	arg1	HS					89:90	HS	89:90	HS	89:90	Heparan sulfate (HS) 3-O-sulfation determines the binding specificity of HS/heparin for antithrombin III and plays a key role in herpes simplex virus (HSV) infection.
25486437	1	8	theme	Heparan	72:78	arg1	sulfate					80:86	Heparan sulfate	72:86	Heparan sulfate (HS) 3-O-sulfation	72:105	Heparan sulfate (HS) 3-O-sulfation determines the binding specificity of HS/heparin for antithrombin III and plays a key role in herpes simplex virus (HSV) infection.
25486437	3	9	theme	multiple	398:405	arg1	isoforms					416:423	multiple distinct isoforms	398:423	multiple distinct isoforms of 3-O-sulfotranserases	398:447	By contrast, multiple distinct isoforms of 3-O-sulfotranserases exist in mammals (up to seven isoenzymes).
25486437	5	10	theme	basic	797:801	arg1	conditions					803:812	mildly basic conditions	790:812	mildly basic conditions	790:812	When HS/heparin is enzymatically depolymerized for compositional analysis, 3-O-sulfated glucosamine at the reducing ends appears to be susceptible to degradation under mildly basic conditions.
25486437	6	11	theme	intermediate	894:905	arg1	products					925:932	the intermediate and side-reaction products	890:932	the intermediate and side-reaction products observed	890:941	We propose a 3-O-desulfation initiated peeling reaction mechanism based on the intermediate and side-reaction products observed.
25486437	1	12	theme	sulfate	80:86	arg1	3-O-sulfation					93:105	Heparan sulfate (HS) 3-O-sulfation	72:105	Heparan sulfate (HS) 3-O-sulfation	72:105	Heparan sulfate (HS) 3-O-sulfation determines the binding specificity of HS/heparin for antithrombin III and plays a key role in herpes simplex virus (HSV) infection.
25486437	3	13	theme	distinct	407:414	arg1	isoforms					416:423	multiple distinct isoforms	398:423	multiple distinct isoforms of 3-O-sulfotranserases	398:447	By contrast, multiple distinct isoforms of 3-O-sulfotranserases exist in mammals (up to seven isoenzymes).
25486437	7	14	theme	peeling	1108:1114	arg1	reaction					1116:1123	this novel peeling reaction	1097:1123	this novel peeling reaction	1097:1123	Our discovery calls for the re-evaluation of the natural abundance and functions of HS 3-O-sulfation by taking into consideration the negative impact of this novel peeling reaction.
25486437	2	15	theme	3-O-sulfation	280:292	arg1	abundance					264:272	the low natural abundance	248:272	the low natural abundance of HS 3-O-sulfation	248:292	However, the low natural abundance of HS 3-O-sulfation poses a serious challenge for functional studies other than the two cases mentioned above.
25486437	4	16	theme	novel	511:515	arg1	reaction					525:532	a novel peeling reaction	509:532	a novel peeling reaction that specifically degrades HS chains with 3-O-sulfated glucosamine at the reducing-end	509:619	Here we describe a novel peeling reaction that specifically degrades HS chains with 3-O-sulfated glucosamine at the reducing-end.
25486437	7	17	theme	reaction	1116:1123	arg1	impact					1087:1092	the negative impact	1074:1092	the negative impact of this novel peeling reaction	1074:1123	Our discovery calls for the re-evaluation of the natural abundance and functions of HS 3-O-sulfation by taking into consideration the negative impact of this novel peeling reaction.
25486437	2	18	theme	HS	277:278	arg1	3-O-sulfation					280:292	HS 3-O-sulfation	277:292	HS 3-O-sulfation	277:292	However, the low natural abundance of HS 3-O-sulfation poses a serious challenge for functional studies other than the two cases mentioned above.
25486437	7	19	dep	abundance	1001:1009	arg1	the					989:991	the	989:991	the	989:991	Our discovery calls for the re-evaluation of the natural abundance and functions of HS 3-O-sulfation by taking into consideration the negative impact of this novel peeling reaction.
25486437	0	20	theme	heparan	15:21	arg1	sulfate					23:29	a heparan sulfate	13:29	a heparan sulfate	13:29	Discovery of a heparan sulfate 3-O-sulfation specific peeling reaction.
25486437	1	21	theme	key	189:191	arg1	role					193:196	a key role	187:196	a key role	187:196	Heparan sulfate (HS) 3-O-sulfation determines the binding specificity of HS/heparin for antithrombin III and plays a key role in herpes simplex virus (HSV) infection.
25486437	3	22	dep	seven	473:477	arg1	to					470:471	to	470:471	to	470:471	By contrast, multiple distinct isoforms of 3-O-sulfotranserases exist in mammals (up to seven isoenzymes).
25486437	5	23	theme	3-O-sulfated	697:708	arg1	glucosamine					710:720	3-O-sulfated glucosamine	697:720	3-O-sulfated glucosamine at the reducing ends	697:741	When HS/heparin is enzymatically depolymerized for compositional analysis, 3-O-sulfated glucosamine at the reducing ends appears to be susceptible to degradation under mildly basic conditions.
25486437	5	24	from	ends	738:741	arg1	glucosamine					710:720	3-O-sulfated glucosamine	697:720	3-O-sulfated glucosamine at the reducing ends	697:741	When HS/heparin is enzymatically depolymerized for compositional analysis, 3-O-sulfated glucosamine at the reducing ends appears to be susceptible to degradation under mildly basic conditions.
25486437	4	25	theme	peeling	517:523	arg1	reaction					525:532	a novel peeling reaction	509:532	a novel peeling reaction that specifically degrades HS chains with 3-O-sulfated glucosamine at the reducing-end	509:619	Here we describe a novel peeling reaction that specifically degrades HS chains with 3-O-sulfated glucosamine at the reducing-end.
25486437	0	26	theme	sulfate	23:29	arg1	Discovery					0:8	Discovery	0:8	Discovery of a heparan sulfate	0:29	Discovery of a heparan sulfate 3-O-sulfation specific peeling reaction.
25486437	1	27	theme	herpes	201:206	arg1	HSV					223:225	HSV	223:225	HSV	223:225	Heparan sulfate (HS) 3-O-sulfation determines the binding specificity of HS/heparin for antithrombin III and plays a key role in herpes simplex virus (HSV) infection.
25486437	1	27	theme	herpes	201:206	arg1	virus					216:220	herpes simplex virus	201:220	herpes simplex virus (HSV) infection	201:236	Heparan sulfate (HS) 3-O-sulfation determines the binding specificity of HS/heparin for antithrombin III and plays a key role in herpes simplex virus (HSV) infection.
25486437	0	28	theme	peeling	54:60	arg1	reaction					62:69	specific peeling reaction	45:69	specific peeling reaction	45:69	Discovery of a heparan sulfate 3-O-sulfation specific peeling reaction.
25486437	6	29	theme	reaction	862:869	arg1	mechanism					871:879	a 3-O-desulfation initiated peeling reaction mechanism	826:879	a 3-O-desulfation initiated peeling reaction mechanism based on the intermediate and side-reaction products observed	826:941	We propose a 3-O-desulfation initiated peeling reaction mechanism based on the intermediate and side-reaction products observed.
25486437	0	30	theme	specific	45:52	arg1	reaction					62:69	specific peeling reaction	45:69	specific peeling reaction	45:69	Discovery of a heparan sulfate 3-O-sulfation specific peeling reaction.
25486437	7	31	theme	negative	1078:1085	arg1	impact					1087:1092	the negative impact	1074:1092	the negative impact of this novel peeling reaction	1074:1123	Our discovery calls for the re-evaluation of the natural abundance and functions of HS 3-O-sulfation by taking into consideration the negative impact of this novel peeling reaction.
25486437	6	32	theme	peeling	854:860	arg1	mechanism					871:879	a 3-O-desulfation initiated peeling reaction mechanism	826:879	a 3-O-desulfation initiated peeling reaction mechanism based on the intermediate and side-reaction products observed	826:941	We propose a 3-O-desulfation initiated peeling reaction mechanism based on the intermediate and side-reaction products observed.
25486437	4	33	with	chains	564:569	arg1	glucosamine					589:599	3-O-sulfated glucosamine	576:599	3-O-sulfated glucosamine	576:599	Here we describe a novel peeling reaction that specifically degrades HS chains with 3-O-sulfated glucosamine at the reducing-end.
25486437	7	34	theme	HS	1028:1029	arg1	3-O-sulfation					1031:1043	HS 3-O-sulfation	1028:1043	HS 3-O-sulfation	1028:1043	Our discovery calls for the re-evaluation of the natural abundance and functions of HS 3-O-sulfation by taking into consideration the negative impact of this novel peeling reaction.
25486437	2	35	theme	serious	302:308	arg1	challenge					310:318	a serious challenge	300:318	a serious challenge for functional studies other than the two cases mentioned above	300:382	However, the low natural abundance of HS 3-O-sulfation poses a serious challenge for functional studies other than the two cases mentioned above.
25486437	5	36	theme	compositional	673:685	arg1	analysis					687:694	compositional analysis	673:694	compositional analysis	673:694	When HS/heparin is enzymatically depolymerized for compositional analysis, 3-O-sulfated glucosamine at the reducing ends appears to be susceptible to degradation under mildly basic conditions.
25486437	6	37	theme	initiated	844:852	arg1	mechanism					871:879	a 3-O-desulfation initiated peeling reaction mechanism	826:879	a 3-O-desulfation initiated peeling reaction mechanism based on the intermediate and side-reaction products observed	826:941	We propose a 3-O-desulfation initiated peeling reaction mechanism based on the intermediate and side-reaction products observed.
25486437	7	38	theme	3-O-sulfation	1031:1043	arg1	functions					1015:1023	functions	1015:1023	functions	1015:1023	Our discovery calls for the re-evaluation of the natural abundance and functions of HS 3-O-sulfation by taking into consideration the negative impact of this novel peeling reaction.
25486437	7	38	theme	3-O-sulfation	1031:1043	arg1	abundance					1001:1009	natural abundance	993:1009	natural abundance	993:1009	Our discovery calls for the re-evaluation of the natural abundance and functions of HS 3-O-sulfation by taking into consideration the negative impact of this novel peeling reaction.
25486437	1	39	theme	binding	122:128	arg1	specificity					130:140	the binding specificity	118:140	the binding specificity of HS/heparin for antithrombin III	118:175	Heparan sulfate (HS) 3-O-sulfation determines the binding specificity of HS/heparin for antithrombin III and plays a key role in herpes simplex virus (HSV) infection.
25486437	1	40	theme	simplex	208:214	arg1	HSV					223:225	HSV	223:225	HSV	223:225	Heparan sulfate (HS) 3-O-sulfation determines the binding specificity of HS/heparin for antithrombin III and plays a key role in herpes simplex virus (HSV) infection.
25486437	1	40	theme	simplex	208:214	arg1	virus					216:220	herpes simplex virus	201:220	herpes simplex virus (HSV) infection	201:236	Heparan sulfate (HS) 3-O-sulfation determines the binding specificity of HS/heparin for antithrombin III and plays a key role in herpes simplex virus (HSV) infection.
25486437	6	41	theme	3-O-desulfation	828:842	arg1	mechanism					871:879	a 3-O-desulfation initiated peeling reaction mechanism	826:879	a 3-O-desulfation initiated peeling reaction mechanism based on the intermediate and side-reaction products observed	826:941	We propose a 3-O-desulfation initiated peeling reaction mechanism based on the intermediate and side-reaction products observed.
25486437	7	42	theme	functions	1015:1023	arg1	re-evaluation					972:984	the re-evaluation	968:984	the re-evaluation of the natural abundance and functions of HS 3-O-sulfation	968:1043	Our discovery calls for the re-evaluation of the natural abundance and functions of HS 3-O-sulfation by taking into consideration the negative impact of this novel peeling reaction.
25486437	1	43	theme	virus	216:220	arg1	infection					228:236	herpes simplex virus (HSV) infection	201:236	herpes simplex virus (HSV) infection	201:236	Heparan sulfate (HS) 3-O-sulfation determines the binding specificity of HS/heparin for antithrombin III and plays a key role in herpes simplex virus (HSV) infection.
25486437	3	44	theme	3-O-sulfotranserases	428:447	arg1	isoforms					416:423	multiple distinct isoforms	398:423	multiple distinct isoforms of 3-O-sulfotranserases	398:447	By contrast, multiple distinct isoforms of 3-O-sulfotranserases exist in mammals (up to seven isoenzymes).
25486437	2	45	theme	other	343:347	arg1	studies					335:341	functional studies	324:341	functional studies other than the two cases mentioned above	324:382	However, the low natural abundance of HS 3-O-sulfation poses a serious challenge for functional studies other than the two cases mentioned above.
25486437	7	46	theme	abundance	1001:1009	arg1	re-evaluation					972:984	the re-evaluation	968:984	the re-evaluation of the natural abundance and functions of HS 3-O-sulfation	968:1043	Our discovery calls for the re-evaluation of the natural abundance and functions of HS 3-O-sulfation by taking into consideration the negative impact of this novel peeling reaction.
25486437	1	47	theme	HS/heparin	145:154	arg1	specificity					130:140	the binding specificity	118:140	the binding specificity of HS/heparin for antithrombin III	118:175	Heparan sulfate (HS) 3-O-sulfation determines the binding specificity of HS/heparin for antithrombin III and plays a key role in herpes simplex virus (HSV) infection.
25486437	4	48	theme	3-O-sulfated	576:587	arg1	glucosamine					589:599	3-O-sulfated glucosamine	576:599	3-O-sulfated glucosamine	576:599	Here we describe a novel peeling reaction that specifically degrades HS chains with 3-O-sulfated glucosamine at the reducing-end.
26483702	14	0	theme	transport	2348:2356	arg1	activity					2358:2365	kidney urea transport activity	2336:2365	kidney urea transport activity	2336:2365	The alteration of these genes may contribute to changes in the UT-A1 glycan structure and therefore modulate kidney urea transport activity and alleviate osmotic diuresis caused by glucosuria in diabetes.
26483702	11	1	theme	protein	1965:1971	arg1	abundance					1973:1981	UT-A1 protein abundance	1959:1981	UT-A1 protein abundance	1959:1981	CONCLUSIONS In diabetes, not only is UT-A1 protein abundance increased but the protein's glycan structure is also significantly changed.
26483702	10	2	dep	expressed	1799:1807	arg1	although					1783:1790	although	1783:1790	although	1783:1790	In contrast, although highly expressed in kidney IM, the glycosyltransferase genes Mgat1, Mgat2, and fucosyltransferase Fut8, did not show any changes.
26483702	14	3	theme	kidney	2336:2341	arg1	activity					2358:2365	kidney urea transport activity	2336:2365	kidney urea transport activity	2336:2365	The alteration of these genes may contribute to changes in the UT-A1 glycan structure and therefore modulate kidney urea transport activity and alleviate osmotic diuresis caused by glucosuria in diabetes.
26483702	7	4	theme	molecular	1151:1159	arg1	basis					1161:1165	the molecular basis	1147:1165	the molecular basis of the alterations of glycan structures	1147:1205	To explore the molecular basis of the alterations of glycan structures, the highly sensitive next generation sequencing (NGS) technology, Illumina RNA-seq, was employed to analyze genes involved in the process of UT-A1 glycosylation using streptozotocin (STZ)-induced diabetic rat kidney.
26483702	7	5	theme	rat	1413:1415	arg1	kidney					1417:1422	streptozotocin (STZ)-induced diabetic rat kidney	1375:1422	streptozotocin (STZ)-induced diabetic rat kidney	1375:1422	To explore the molecular basis of the alterations of glycan structures, the highly sensitive next generation sequencing (NGS) technology, Illumina RNA-seq, was employed to analyze genes involved in the process of UT-A1 glycosylation using streptozotocin (STZ)-induced diabetic rat kidney.
26483702	9	6	theme	sialylation	1675:1685	arg1	St3gal4					1707:1713	St3gal4	1707:1713	St3gal4	1707:1713	These genes include the glycosyltransferase genes Mgat4a, the sialylation enzymes St3gal1 and St3gal4 and glycan binding protein galectin-3, -5, -8, and -9.
26483702	9	6	theme	sialylation	1675:1685	arg1	St3gal1					1695:1701	St3gal1	1695:1701	St3gal1	1695:1701	These genes include the glycosyltransferase genes Mgat4a, the sialylation enzymes St3gal1 and St3gal4 and glycan binding protein galectin-3, -5, -8, and -9.
26483702	9	6	theme	sialylation	1675:1685	arg1	enzymes					1687:1693	the sialylation enzymes St3gal1 and St3gal4	1671:1713	the sialylation enzymes St3gal1 and St3gal4	1671:1713	These genes include the glycosyltransferase genes Mgat4a, the sialylation enzymes St3gal1 and St3gal4 and glycan binding protein galectin-3, -5, -8, and -9.
26483702	4	7	theme	carbohydrate	629:640	arg1	change					652:657	the glycan carbohydrate structure change	618:657	the glycan carbohydrate structure change	618:657	However, how the glycan carbohydrate structure change and the glycan related enzymes regulate kidney urea transport activity, particularly under diabetic condition, is largely unknown.
26483702	3	8	theme	important	533:541	arg1	role					543:546	an important role	530:546	an important role	530:546	In diabetes, UT-A1 protein abundance, particularly the 117 kD isoform, is significantly increased corresponding to an increased urea permeability in perfused IM collecting ducts, which plays an important role in preventing the osmotic diuresis caused by glucosuria.
26483702	11	9	theme	glycan	2011:2016	arg1	structure					2018:2026	the protein's glycan structure	1997:2026	the protein's glycan structure	1997:2026	CONCLUSIONS In diabetes, not only is UT-A1 protein abundance increased but the protein's glycan structure is also significantly changed.
26483702	7	10	theme	UT-A1	1349:1353	arg1	glycosylation					1355:1367	UT-A1 glycosylation	1349:1367	UT-A1 glycosylation	1349:1367	To explore the molecular basis of the alterations of glycan structures, the highly sensitive next generation sequencing (NGS) technology, Illumina RNA-seq, was employed to analyze genes involved in the process of UT-A1 glycosylation using streptozotocin (STZ)-induced diabetic rat kidney.
26483702	12	11	theme	UT-A1	2059:2063	arg1	protein					2065:2071	UT-A1 protein	2059:2071	UT-A1 protein	2059:2071	UT-A1 protein becomes highly sialylated, fucosylated and branched.
26483702	2	12	theme	glycosylated	270:281	arg1	protein					283:289	a heavily glycosylated protein	260:289	a heavily glycosylated protein with two glycosylation forms of 97 and 117 kDa	260:336	Native UT-A1 from kidney inner medulla (IM) is a heavily glycosylated protein with two glycosylation forms of 97 and 117 kDa.
26483702	2	12	theme	glycosylated	270:281	arg1	UT-A1					220:224	Native UT-A1	213:224	Native UT-A1 from kidney inner medulla (IM)	213:255	Native UT-A1 from kidney inner medulla (IM) is a heavily glycosylated protein with two glycosylation forms of 97 and 117 kDa.
26483702	1	13	theme	concentrated	193:204	arg1	urine					206:210	concentrated urine	193:210	concentrated urine	193:210	UNLABELLED The UT-A1 urea transporter is crucial to the kidney's ability to generate concentrated urine.
26483702	14	14	from	glucosuria	2408:2417	arg1	diabetes					2422:2429	diabetes	2422:2429	diabetes	2422:2429	The alteration of these genes may contribute to changes in the UT-A1 glycan structure and therefore modulate kidney urea transport activity and alleviate osmotic diuresis caused by glucosuria in diabetes.
26483702	11	15	from	CONCLUSIONS	1922:1932	arg1	diabetes					1937:1944	diabetes	1937:1944	diabetes	1937:1944	CONCLUSIONS In diabetes, not only is UT-A1 protein abundance increased but the protein's glycan structure is also significantly changed.
26483702	5	16	theme	sugar-specific	811:824	arg1	lectins					834:840	sugar-specific binding lectins	811:840	sugar-specific binding lectins	811:840	In this study, using sugar-specific binding lectins, we found that the carbohydrate structure of UT-A1 is changed with increased amounts of sialic acid, fucose, and increased glycan branching under diabetic conditions.
26483702	9	17	theme	glycan	1719:1724	arg1	genes					1657:1661	the glycosyltransferase genes Mgat4a, the sialylation enzymes St3gal1 and St3gal4 and glycan binding protein galectin-3	1633:1751	the glycosyltransferase genes Mgat4a, the sialylation enzymes St3gal1 and St3gal4 and glycan binding protein galectin-3	1633:1751	These genes include the glycosyltransferase genes Mgat4a, the sialylation enzymes St3gal1 and St3gal4 and glycan binding protein galectin-3, -5, -8, and -9.
26483702	9	17	theme	glycan	1719:1724	arg1	galectin-3					1742:1751	glycan binding protein galectin-3	1719:1751	glycan binding protein galectin-3	1719:1751	These genes include the glycosyltransferase genes Mgat4a, the sialylation enzymes St3gal1 and St3gal4 and glycan binding protein galectin-3, -5, -8, and -9.
26483702	4	18	theme	related	674:680	arg1	enzymes					682:688	the glycan related enzymes	663:688	the glycan related enzymes	663:688	However, how the glycan carbohydrate structure change and the glycan related enzymes regulate kidney urea transport activity, particularly under diabetic condition, is largely unknown.
26483702	2	19	theme	Native	213:218	arg1	protein					283:289	a heavily glycosylated protein	260:289	a heavily glycosylated protein with two glycosylation forms of 97 and 117 kDa	260:336	Native UT-A1 from kidney inner medulla (IM) is a heavily glycosylated protein with two glycosylation forms of 97 and 117 kDa.
26483702	2	19	theme	Native	213:218	arg1	UT-A1					220:224	Native UT-A1	213:224	Native UT-A1 from kidney inner medulla (IM)	213:255	Native UT-A1 from kidney inner medulla (IM) is a heavily glycosylated protein with two glycosylation forms of 97 and 117 kDa.
26483702	1	20	theme	UT-A1	123:127	arg1	transporter					134:144	UNLABELLED The UT-A1 urea transporter	108:144	UNLABELLED The UT-A1 urea transporter	108:144	UNLABELLED The UT-A1 urea transporter is crucial to the kidney's ability to generate concentrated urine.
26483702	9	21	theme	protein	1734:1740	arg1	genes					1657:1661	the glycosyltransferase genes Mgat4a, the sialylation enzymes St3gal1 and St3gal4 and glycan binding protein galectin-3	1633:1751	the glycosyltransferase genes Mgat4a, the sialylation enzymes St3gal1 and St3gal4 and glycan binding protein galectin-3	1633:1751	These genes include the glycosyltransferase genes Mgat4a, the sialylation enzymes St3gal1 and St3gal4 and glycan binding protein galectin-3, -5, -8, and -9.
26483702	9	21	theme	protein	1734:1740	arg1	galectin-3					1742:1751	glycan binding protein galectin-3	1719:1751	glycan binding protein galectin-3	1719:1751	These genes include the glycosyltransferase genes Mgat4a, the sialylation enzymes St3gal1 and St3gal4 and glycan binding protein galectin-3, -5, -8, and -9.
26483702	8	22	theme	Differential	1425:1436	arg1	analysis					1454:1461	Differential gene expression analysis	1425:1461	Differential gene expression analysis combining with quantitative PCR	1425:1493	Differential gene expression analysis combining with quantitative PCR revealed that expression of a number of important glycosylation related genes were changed under diabetic conditions.
26483702	14	23	theme	glycan	2296:2301	arg1	structure					2303:2311	the UT-A1 glycan structure	2286:2311	the UT-A1 glycan structure	2286:2311	The alteration of these genes may contribute to changes in the UT-A1 glycan structure and therefore modulate kidney urea transport activity and alleviate osmotic diuresis caused by glucosuria in diabetes.
26483702	5	24	theme	UT-A1	887:891	arg1	structure					874:882	the carbohydrate structure	857:882	the carbohydrate structure of UT-A1	857:891	In this study, using sugar-specific binding lectins, we found that the carbohydrate structure of UT-A1 is changed with increased amounts of sialic acid, fucose, and increased glycan branching under diabetic conditions.
26483702	8	25	theme	expression	1443:1452	arg1	analysis					1454:1461	Differential gene expression analysis	1425:1461	Differential gene expression analysis combining with quantitative PCR	1425:1493	Differential gene expression analysis combining with quantitative PCR revealed that expression of a number of important glycosylation related genes were changed under diabetic conditions.
26483702	0	26	theme	STZ-induced	61:71	arg1	medulla					99:105	STZ-induced diabetic rat kidney inner medulla	61:105	STZ-induced diabetic rat kidney inner medulla	61:105	RNA-seq analysis of glycosylation related gene expression in STZ-induced diabetic rat kidney inner medulla.
26483702	2	27	theme	inner	238:242	arg1	medulla					244:250	kidney inner medulla	231:250	kidney inner medulla (IM)	231:255	Native UT-A1 from kidney inner medulla (IM) is a heavily glycosylated protein with two glycosylation forms of 97 and 117 kDa.
26483702	2	27	theme	inner	238:242	arg1	IM					253:254	IM	253:254	IM	253:254	Native UT-A1 from kidney inner medulla (IM) is a heavily glycosylated protein with two glycosylation forms of 97 and 117 kDa.
26483702	0	28	theme	rat	82:84	arg1	medulla					99:105	STZ-induced diabetic rat kidney inner medulla	61:105	STZ-induced diabetic rat kidney inner medulla	61:105	RNA-seq analysis of glycosylation related gene expression in STZ-induced diabetic rat kidney inner medulla.
26483702	5	29	theme	carbohydrate	861:872	arg1	structure					874:882	the carbohydrate structure	857:882	the carbohydrate structure of UT-A1	857:891	In this study, using sugar-specific binding lectins, we found that the carbohydrate structure of UT-A1 is changed with increased amounts of sialic acid, fucose, and increased glycan branching under diabetic conditions.
26483702	8	30	theme	quantitative	1478:1489	arg1	PCR					1491:1493	quantitative PCR	1478:1493	quantitative PCR	1478:1493	Differential gene expression analysis combining with quantitative PCR revealed that expression of a number of important glycosylation related genes were changed under diabetic conditions.
26483702	0	31	theme	inner	93:97	arg1	medulla					99:105	STZ-induced diabetic rat kidney inner medulla	61:105	STZ-induced diabetic rat kidney inner medulla	61:105	RNA-seq analysis of glycosylation related gene expression in STZ-induced diabetic rat kidney inner medulla.
26483702	5	32	theme	sialic	930:935	arg1	acid					937:940	sialic acid	930:940	sialic acid	930:940	In this study, using sugar-specific binding lectins, we found that the carbohydrate structure of UT-A1 is changed with increased amounts of sialic acid, fucose, and increased glycan branching under diabetic conditions.
26483702	0	33	from	expression	47:56	arg1	medulla					99:105	STZ-induced diabetic rat kidney inner medulla	61:105	STZ-induced diabetic rat kidney inner medulla	61:105	RNA-seq analysis of glycosylation related gene expression in STZ-induced diabetic rat kidney inner medulla.
26483702	4	34	theme	diabetic	750:757	arg1	condition					759:767	diabetic condition	750:767	diabetic condition	750:767	However, how the glycan carbohydrate structure change and the glycan related enzymes regulate kidney urea transport activity, particularly under diabetic condition, is largely unknown.
26483702	14	35	theme	genes	2251:2255	arg1	alteration					2231:2240	The alteration	2227:2240	The alteration of these genes	2227:2255	The alteration of these genes may contribute to changes in the UT-A1 glycan structure and therefore modulate kidney urea transport activity and alleviate osmotic diuresis caused by glucosuria in diabetes.
26483702	3	36	theme	kD	398:399	arg1	abundance					366:374	UT-A1 protein abundance	352:374	UT-A1 protein abundance	352:374	In diabetes, UT-A1 protein abundance, particularly the 117 kD isoform, is significantly increased corresponding to an increased urea permeability in perfused IM collecting ducts, which plays an important role in preventing the osmotic diuresis caused by glucosuria.
26483702	3	36	theme	kD	398:399	arg1	isoform					401:407	the 117 kD isoform	390:407	particularly the 117 kD isoform	377:407	In diabetes, UT-A1 protein abundance, particularly the 117 kD isoform, is significantly increased corresponding to an increased urea permeability in perfused IM collecting ducts, which plays an important role in preventing the osmotic diuresis caused by glucosuria.
26483702	0	37	theme	diabetic	73:80	arg1	medulla					99:105	STZ-induced diabetic rat kidney inner medulla	61:105	STZ-induced diabetic rat kidney inner medulla	61:105	RNA-seq analysis of glycosylation related gene expression in STZ-induced diabetic rat kidney inner medulla.
26483702	4	38	theme	urea	706:709	arg1	transport					711:719	kidney urea transport	699:719	kidney urea transport activity	699:728	However, how the glycan carbohydrate structure change and the glycan related enzymes regulate kidney urea transport activity, particularly under diabetic condition, is largely unknown.
26483702	0	39	theme	RNA-seq	0:6	arg1	analysis					8:15	RNA-seq analysis	0:15	RNA-seq analysis of glycosylation	0:32	RNA-seq analysis of glycosylation related gene expression in STZ-induced diabetic rat kidney inner medulla.
26483702	2	40	from	medulla	244:250	arg1	protein					283:289	a heavily glycosylated protein	260:289	a heavily glycosylated protein with two glycosylation forms of 97 and 117 kDa	260:336	Native UT-A1 from kidney inner medulla (IM) is a heavily glycosylated protein with two glycosylation forms of 97 and 117 kDa.
26483702	2	40	from	medulla	244:250	arg1	UT-A1					220:224	Native UT-A1	213:224	Native UT-A1 from kidney inner medulla (IM)	213:255	Native UT-A1 from kidney inner medulla (IM) is a heavily glycosylated protein with two glycosylation forms of 97 and 117 kDa.
26483702	1	41	theme	UNLABELLED	108:117	arg1	transporter					134:144	UNLABELLED The UT-A1 urea transporter	108:144	UNLABELLED The UT-A1 urea transporter	108:144	UNLABELLED The UT-A1 urea transporter is crucial to the kidney's ability to generate concentrated urine.
26483702	7	42	theme	Illumina	1274:1281	arg1	generation					1234:1243	the highly sensitive next generation	1208:1243	the highly sensitive next generation sequencing (NGS) technology	1208:1271	To explore the molecular basis of the alterations of glycan structures, the highly sensitive next generation sequencing (NGS) technology, Illumina RNA-seq, was employed to analyze genes involved in the process of UT-A1 glycosylation using streptozotocin (STZ)-induced diabetic rat kidney.
26483702	7	42	theme	Illumina	1274:1281	arg1	RNA-seq					1283:1289	Illumina RNA-seq	1274:1289	Illumina RNA-seq	1274:1289	To explore the molecular basis of the alterations of glycan structures, the highly sensitive next generation sequencing (NGS) technology, Illumina RNA-seq, was employed to analyze genes involved in the process of UT-A1 glycosylation using streptozotocin (STZ)-induced diabetic rat kidney.
26483702	9	43	dep	enzymes	1687:1693	arg1	St3gal4					1707:1713	St3gal4	1707:1713	St3gal4	1707:1713	These genes include the glycosyltransferase genes Mgat4a, the sialylation enzymes St3gal1 and St3gal4 and glycan binding protein galectin-3, -5, -8, and -9.
26483702	9	43	dep	enzymes	1687:1693	arg1	St3gal1					1695:1701	St3gal1	1695:1701	St3gal1	1695:1701	These genes include the glycosyltransferase genes Mgat4a, the sialylation enzymes St3gal1 and St3gal4 and glycan binding protein galectin-3, -5, -8, and -9.
26483702	9	43	dep	enzymes	1687:1693	arg1	enzymes					1687:1693	the sialylation enzymes St3gal1 and St3gal4	1671:1713	the sialylation enzymes St3gal1 and St3gal4	1671:1713	These genes include the glycosyltransferase genes Mgat4a, the sialylation enzymes St3gal1 and St3gal4 and glycan binding protein galectin-3, -5, -8, and -9.
26483702	13	44	theme	crucial	2152:2158	arg1	genes					2182:2186	crucial glycosylation related genes	2152:2186	crucial glycosylation related genes	2152:2186	Consistently, a number of crucial glycosylation related genes are changed under diabetic conditions.
26483702	5	45	theme	branching	972:980	arg1	branching					972:980	increased glycan branching	955:980	increased glycan branching	955:980	In this study, using sugar-specific binding lectins, we found that the carbohydrate structure of UT-A1 is changed with increased amounts of sialic acid, fucose, and increased glycan branching under diabetic conditions.
26483702	5	45	theme	branching	972:980	arg1	fucose					943:948	fucose	943:948	fucose	943:948	In this study, using sugar-specific binding lectins, we found that the carbohydrate structure of UT-A1 is changed with increased amounts of sialic acid, fucose, and increased glycan branching under diabetic conditions.
26483702	5	45	theme	branching	972:980	arg1	amounts					919:925	increased amounts	909:925	increased amounts of sialic acid, fucose, and increased glycan branching	909:980	In this study, using sugar-specific binding lectins, we found that the carbohydrate structure of UT-A1 is changed with increased amounts of sialic acid, fucose, and increased glycan branching under diabetic conditions.
26483702	5	45	theme	branching	972:980	arg1	acid					937:940	sialic acid	930:940	sialic acid	930:940	In this study, using sugar-specific binding lectins, we found that the carbohydrate structure of UT-A1 is changed with increased amounts of sialic acid, fucose, and increased glycan branching under diabetic conditions.
26483702	3	46	theme	protein	358:364	arg1	abundance					366:374	UT-A1 protein abundance	352:374	UT-A1 protein abundance	352:374	In diabetes, UT-A1 protein abundance, particularly the 117 kD isoform, is significantly increased corresponding to an increased urea permeability in perfused IM collecting ducts, which plays an important role in preventing the osmotic diuresis caused by glucosuria.
26483702	3	46	theme	protein	358:364	arg1	isoform					401:407	the 117 kD isoform	390:407	particularly the 117 kD isoform	377:407	In diabetes, UT-A1 protein abundance, particularly the 117 kD isoform, is significantly increased corresponding to an increased urea permeability in perfused IM collecting ducts, which plays an important role in preventing the osmotic diuresis caused by glucosuria.
26483702	8	47	theme	glycosylation	1545:1557	arg1	genes					1567:1571	important glycosylation related genes	1535:1571	important glycosylation related genes	1535:1571	Differential gene expression analysis combining with quantitative PCR revealed that expression of a number of important glycosylation related genes were changed under diabetic conditions.
26483702	13	48	theme	related	2174:2180	arg1	genes					2182:2186	crucial glycosylation related genes	2152:2186	crucial glycosylation related genes	2152:2186	Consistently, a number of crucial glycosylation related genes are changed under diabetic conditions.
26483702	5	49	theme	diabetic	988:995	arg1	conditions					997:1006	diabetic conditions	988:1006	diabetic conditions	988:1006	In this study, using sugar-specific binding lectins, we found that the carbohydrate structure of UT-A1 is changed with increased amounts of sialic acid, fucose, and increased glycan branching under diabetic conditions.
26483702	7	50	theme	NGS	1257:1259	arg1	technology					1262:1271	sequencing (NGS) technology	1245:1271	the highly sensitive next generation sequencing (NGS) technology	1208:1271	To explore the molecular basis of the alterations of glycan structures, the highly sensitive next generation sequencing (NGS) technology, Illumina RNA-seq, was employed to analyze genes involved in the process of UT-A1 glycosylation using streptozotocin (STZ)-induced diabetic rat kidney.
26483702	8	51	theme	genes	1567:1571	arg1	number					1525:1530	a number	1523:1530	a number of important glycosylation related genes	1523:1571	Differential gene expression analysis combining with quantitative PCR revealed that expression of a number of important glycosylation related genes were changed under diabetic conditions.
26483702	3	52	theme	collecting	500:509	arg1	ducts					511:515	perfused IM collecting ducts	488:515	perfused IM collecting ducts	488:515	In diabetes, UT-A1 protein abundance, particularly the 117 kD isoform, is significantly increased corresponding to an increased urea permeability in perfused IM collecting ducts, which plays an important role in preventing the osmotic diuresis caused by glucosuria.
26483702	6	53	theme	galectin	1078:1085	arg1	proteins					1126:1133	β-galactoside glycan binding proteins	1097:1133	β-galactoside glycan binding proteins	1097:1133	These changes were accompanied by altered UT-A1 association with the galectin proteins, β-galactoside glycan binding proteins.
26483702	6	53	theme	galectin	1078:1085	arg1	proteins					1087:1094	the galectin proteins	1074:1094	the galectin proteins	1074:1094	These changes were accompanied by altered UT-A1 association with the galectin proteins, β-galactoside glycan binding proteins.
26483702	10	54	dep	genes	1847:1851	arg1	Mgat1					1853:1857	Mgat1	1853:1857	Mgat1	1853:1857	In contrast, although highly expressed in kidney IM, the glycosyltransferase genes Mgat1, Mgat2, and fucosyltransferase Fut8, did not show any changes.
26483702	10	54	dep	genes	1847:1851	arg1	Fut8					1890:1893	fucosyltransferase Fut8	1871:1893	fucosyltransferase Fut8	1871:1893	In contrast, although highly expressed in kidney IM, the glycosyltransferase genes Mgat1, Mgat2, and fucosyltransferase Fut8, did not show any changes.
26483702	10	54	dep	genes	1847:1851	arg1	genes					1847:1851	the glycosyltransferase genes Mgat1, Mgat2, and fucosyltransferase Fut8	1823:1893	the glycosyltransferase genes Mgat1, Mgat2, and fucosyltransferase Fut8	1823:1893	In contrast, although highly expressed in kidney IM, the glycosyltransferase genes Mgat1, Mgat2, and fucosyltransferase Fut8, did not show any changes.
26483702	10	54	dep	genes	1847:1851	arg1	Mgat2					1860:1864	Mgat2	1860:1864	Mgat2	1860:1864	In contrast, although highly expressed in kidney IM, the glycosyltransferase genes Mgat1, Mgat2, and fucosyltransferase Fut8, did not show any changes.
26483702	5	55	theme	increased	955:963	arg1	branching					972:980	increased glycan branching	955:980	increased glycan branching	955:980	In this study, using sugar-specific binding lectins, we found that the carbohydrate structure of UT-A1 is changed with increased amounts of sialic acid, fucose, and increased glycan branching under diabetic conditions.
26483702	7	56	theme	next	1229:1232	arg1	RNA-seq					1283:1289	Illumina RNA-seq	1274:1289	Illumina RNA-seq	1274:1289	To explore the molecular basis of the alterations of glycan structures, the highly sensitive next generation sequencing (NGS) technology, Illumina RNA-seq, was employed to analyze genes involved in the process of UT-A1 glycosylation using streptozotocin (STZ)-induced diabetic rat kidney.
26483702	7	56	theme	next	1229:1232	arg1	generation					1234:1243	the highly sensitive next generation	1208:1243	the highly sensitive next generation sequencing (NGS) technology	1208:1271	To explore the molecular basis of the alterations of glycan structures, the highly sensitive next generation sequencing (NGS) technology, Illumina RNA-seq, was employed to analyze genes involved in the process of UT-A1 glycosylation using streptozotocin (STZ)-induced diabetic rat kidney.
26483702	8	57	theme	diabetic	1592:1599	arg1	conditions					1601:1610	diabetic conditions	1592:1610	diabetic conditions	1592:1610	Differential gene expression analysis combining with quantitative PCR revealed that expression of a number of important glycosylation related genes were changed under diabetic conditions.
26483702	2	58	gly	glycosylated	270:281	arg1	protein					283:289	a heavily glycosylated protein	260:289	a heavily glycosylated protein with two glycosylation forms of 97 and 117 kDa	260:336	Native UT-A1 from kidney inner medulla (IM) is a heavily glycosylated protein with two glycosylation forms of 97 and 117 kDa.
26483702	2	58	gly	glycosylated	270:281	arg1	UT-A1					220:224	Native UT-A1	213:224	Native UT-A1 from kidney inner medulla (IM)	213:255	Native UT-A1 from kidney inner medulla (IM) is a heavily glycosylated protein with two glycosylation forms of 97 and 117 kDa.
26483702	10	59	theme	fucosyltransferase	1871:1888	arg1	Fut8					1890:1893	fucosyltransferase Fut8	1871:1893	fucosyltransferase Fut8	1871:1893	In contrast, although highly expressed in kidney IM, the glycosyltransferase genes Mgat1, Mgat2, and fucosyltransferase Fut8, did not show any changes.
26483702	10	59	theme	fucosyltransferase	1871:1888	arg1	genes					1847:1851	the glycosyltransferase genes Mgat1, Mgat2, and fucosyltransferase Fut8	1823:1893	the glycosyltransferase genes Mgat1, Mgat2, and fucosyltransferase Fut8	1823:1893	In contrast, although highly expressed in kidney IM, the glycosyltransferase genes Mgat1, Mgat2, and fucosyltransferase Fut8, did not show any changes.
26483702	3	60	theme	urea	467:470	arg1	permeability					472:483	an increased urea permeability	454:483	an increased urea permeability in perfused IM collecting ducts, which plays an important role in preventing the osmotic diuresis caused by glucosuria	454:602	In diabetes, UT-A1 protein abundance, particularly the 117 kD isoform, is significantly increased corresponding to an increased urea permeability in perfused IM collecting ducts, which plays an important role in preventing the osmotic diuresis caused by glucosuria.
26483702	9	61	theme	glycosyltransferase	1637:1655	arg1	galectin-3					1742:1751	glycan binding protein galectin-3	1719:1751	glycan binding protein galectin-3	1719:1751	These genes include the glycosyltransferase genes Mgat4a, the sialylation enzymes St3gal1 and St3gal4 and glycan binding protein galectin-3, -5, -8, and -9.
26483702	9	61	theme	glycosyltransferase	1637:1655	arg1	genes					1657:1661	the glycosyltransferase genes Mgat4a, the sialylation enzymes St3gal1 and St3gal4 and glycan binding protein galectin-3	1633:1751	the glycosyltransferase genes Mgat4a, the sialylation enzymes St3gal1 and St3gal4 and glycan binding protein galectin-3	1633:1751	These genes include the glycosyltransferase genes Mgat4a, the sialylation enzymes St3gal1 and St3gal4 and glycan binding protein galectin-3, -5, -8, and -9.
26483702	9	61	theme	glycosyltransferase	1637:1655	arg1	Mgat4a					1663:1668	Mgat4a	1663:1668	Mgat4a	1663:1668	These genes include the glycosyltransferase genes Mgat4a, the sialylation enzymes St3gal1 and St3gal4 and glycan binding protein galectin-3, -5, -8, and -9.
26483702	9	61	theme	glycosyltransferase	1637:1655	arg1	-9					1766:1767	-9	1766:1767	-9	1766:1767	These genes include the glycosyltransferase genes Mgat4a, the sialylation enzymes St3gal1 and St3gal4 and glycan binding protein galectin-3, -5, -8, and -9.
26483702	9	61	theme	glycosyltransferase	1637:1655	arg1	-5					1754:1755	-5	1754:1755	-5	1754:1755	These genes include the glycosyltransferase genes Mgat4a, the sialylation enzymes St3gal1 and St3gal4 and glycan binding protein galectin-3, -5, -8, and -9.
26483702	9	61	theme	glycosyltransferase	1637:1655	arg1	-8					1758:1759	-8	1758:1759	-8	1758:1759	These genes include the glycosyltransferase genes Mgat4a, the sialylation enzymes St3gal1 and St3gal4 and glycan binding protein galectin-3, -5, -8, and -9.
26483702	6	62	theme	UT-A1	1051:1055	arg1	association					1057:1067	altered UT-A1 association	1043:1067	altered UT-A1 association with the galectin proteins, β-galactoside glycan binding proteins	1043:1133	These changes were accompanied by altered UT-A1 association with the galectin proteins, β-galactoside glycan binding proteins.
26483702	3	63	from	permeability	472:483	arg1	ducts					511:515	perfused IM collecting ducts	488:515	perfused IM collecting ducts	488:515	In diabetes, UT-A1 protein abundance, particularly the 117 kD isoform, is significantly increased corresponding to an increased urea permeability in perfused IM collecting ducts, which plays an important role in preventing the osmotic diuresis caused by glucosuria.
26483702	11	64	theme	UT-A1	1959:1963	arg1	abundance					1973:1981	UT-A1 protein abundance	1959:1981	UT-A1 protein abundance	1959:1981	CONCLUSIONS In diabetes, not only is UT-A1 protein abundance increased but the protein's glycan structure is also significantly changed.
26483702	6	65	with	association	1057:1067	arg1	proteins					1126:1133	β-galactoside glycan binding proteins	1097:1133	β-galactoside glycan binding proteins	1097:1133	These changes were accompanied by altered UT-A1 association with the galectin proteins, β-galactoside glycan binding proteins.
26483702	6	65	with	association	1057:1067	arg1	proteins					1087:1094	the galectin proteins	1074:1094	the galectin proteins	1074:1094	These changes were accompanied by altered UT-A1 association with the galectin proteins, β-galactoside glycan binding proteins.
26483702	7	66	theme	-induced	1395:1402	arg1	kidney					1417:1422	streptozotocin (STZ)-induced diabetic rat kidney	1375:1422	streptozotocin (STZ)-induced diabetic rat kidney	1375:1422	To explore the molecular basis of the alterations of glycan structures, the highly sensitive next generation sequencing (NGS) technology, Illumina RNA-seq, was employed to analyze genes involved in the process of UT-A1 glycosylation using streptozotocin (STZ)-induced diabetic rat kidney.
26483702	14	67	theme	urea	2343:2346	arg1	activity					2358:2365	kidney urea transport activity	2336:2365	kidney urea transport activity	2336:2365	The alteration of these genes may contribute to changes in the UT-A1 glycan structure and therefore modulate kidney urea transport activity and alleviate osmotic diuresis caused by glucosuria in diabetes.
26483702	3	68	theme	IM	497:498	arg1	ducts					511:515	perfused IM collecting ducts	488:515	perfused IM collecting ducts	488:515	In diabetes, UT-A1 protein abundance, particularly the 117 kD isoform, is significantly increased corresponding to an increased urea permeability in perfused IM collecting ducts, which plays an important role in preventing the osmotic diuresis caused by glucosuria.
26483702	5	69	theme	binding	826:832	arg1	lectins					834:840	sugar-specific binding lectins	811:840	sugar-specific binding lectins	811:840	In this study, using sugar-specific binding lectins, we found that the carbohydrate structure of UT-A1 is changed with increased amounts of sialic acid, fucose, and increased glycan branching under diabetic conditions.
26483702	7	70	theme	diabetic	1404:1411	arg1	kidney					1417:1422	streptozotocin (STZ)-induced diabetic rat kidney	1375:1422	streptozotocin (STZ)-induced diabetic rat kidney	1375:1422	To explore the molecular basis of the alterations of glycan structures, the highly sensitive next generation sequencing (NGS) technology, Illumina RNA-seq, was employed to analyze genes involved in the process of UT-A1 glycosylation using streptozotocin (STZ)-induced diabetic rat kidney.
26483702	7	71	theme	glycan	1189:1194	arg1	structures					1196:1205	glycan structures	1189:1205	glycan structures	1189:1205	To explore the molecular basis of the alterations of glycan structures, the highly sensitive next generation sequencing (NGS) technology, Illumina RNA-seq, was employed to analyze genes involved in the process of UT-A1 glycosylation using streptozotocin (STZ)-induced diabetic rat kidney.
26483702	4	72	theme	glycan	622:627	arg1	change					652:657	the glycan carbohydrate structure change	618:657	the glycan carbohydrate structure change	618:657	However, how the glycan carbohydrate structure change and the glycan related enzymes regulate kidney urea transport activity, particularly under diabetic condition, is largely unknown.
26483702	7	73	theme	glycosylation	1355:1367	arg1	process					1338:1344	the process	1334:1344	the process of UT-A1 glycosylation	1334:1367	To explore the molecular basis of the alterations of glycan structures, the highly sensitive next generation sequencing (NGS) technology, Illumina RNA-seq, was employed to analyze genes involved in the process of UT-A1 glycosylation using streptozotocin (STZ)-induced diabetic rat kidney.
26483702	2	74	theme	kidney	231:236	arg1	medulla					244:250	kidney inner medulla	231:250	kidney inner medulla (IM)	231:255	Native UT-A1 from kidney inner medulla (IM) is a heavily glycosylated protein with two glycosylation forms of 97 and 117 kDa.
26483702	2	74	theme	kidney	231:236	arg1	IM					253:254	IM	253:254	IM	253:254	Native UT-A1 from kidney inner medulla (IM) is a heavily glycosylated protein with two glycosylation forms of 97 and 117 kDa.
26483702	1	75	theme	The	119:121	arg1	transporter					134:144	UNLABELLED The UT-A1 urea transporter	108:144	UNLABELLED The UT-A1 urea transporter	108:144	UNLABELLED The UT-A1 urea transporter is crucial to the kidney's ability to generate concentrated urine.
26483702	9	76	theme	binding	1726:1732	arg1	genes					1657:1661	the glycosyltransferase genes Mgat4a, the sialylation enzymes St3gal1 and St3gal4 and glycan binding protein galectin-3	1633:1751	the glycosyltransferase genes Mgat4a, the sialylation enzymes St3gal1 and St3gal4 and glycan binding protein galectin-3	1633:1751	These genes include the glycosyltransferase genes Mgat4a, the sialylation enzymes St3gal1 and St3gal4 and glycan binding protein galectin-3, -5, -8, and -9.
26483702	9	76	theme	binding	1726:1732	arg1	galectin-3					1742:1751	glycan binding protein galectin-3	1719:1751	glycan binding protein galectin-3	1719:1751	These genes include the glycosyltransferase genes Mgat4a, the sialylation enzymes St3gal1 and St3gal4 and glycan binding protein galectin-3, -5, -8, and -9.
26483702	8	77	theme	gene	1438:1441	arg1	analysis					1454:1461	Differential gene expression analysis	1425:1461	Differential gene expression analysis combining with quantitative PCR	1425:1493	Differential gene expression analysis combining with quantitative PCR revealed that expression of a number of important glycosylation related genes were changed under diabetic conditions.
26483702	4	78	theme	glycan	667:672	arg1	enzymes					682:688	the glycan related enzymes	663:688	the glycan related enzymes	663:688	However, how the glycan carbohydrate structure change and the glycan related enzymes regulate kidney urea transport activity, particularly under diabetic condition, is largely unknown.
26483702	10	79	theme	kidney	1812:1817	arg1	IM					1819:1820	kidney IM	1812:1820	kidney IM	1812:1820	In contrast, although highly expressed in kidney IM, the glycosyltransferase genes Mgat1, Mgat2, and fucosyltransferase Fut8, did not show any changes.
26483702	10	79	theme	kidney	1812:1817	arg1	genes					1847:1851	the glycosyltransferase genes Mgat1, Mgat2, and fucosyltransferase Fut8	1823:1893	the glycosyltransferase genes Mgat1, Mgat2, and fucosyltransferase Fut8	1823:1893	In contrast, although highly expressed in kidney IM, the glycosyltransferase genes Mgat1, Mgat2, and fucosyltransferase Fut8, did not show any changes.
26483702	1	80	theme	urea	129:132	arg1	transporter					134:144	UNLABELLED The UT-A1 urea transporter	108:144	UNLABELLED The UT-A1 urea transporter	108:144	UNLABELLED The UT-A1 urea transporter is crucial to the kidney's ability to generate concentrated urine.
26483702	6	81	theme	binding	1118:1124	arg1	proteins					1126:1133	β-galactoside glycan binding proteins	1097:1133	β-galactoside glycan binding proteins	1097:1133	These changes were accompanied by altered UT-A1 association with the galectin proteins, β-galactoside glycan binding proteins.
26483702	6	81	theme	binding	1118:1124	arg1	proteins					1087:1094	the galectin proteins	1074:1094	the galectin proteins	1074:1094	These changes were accompanied by altered UT-A1 association with the galectin proteins, β-galactoside glycan binding proteins.
26483702	14	82	theme	UT-A1	2290:2294	arg1	structure					2303:2311	the UT-A1 glycan structure	2286:2311	the UT-A1 glycan structure	2286:2311	The alteration of these genes may contribute to changes in the UT-A1 glycan structure and therefore modulate kidney urea transport activity and alleviate osmotic diuresis caused by glucosuria in diabetes.
26483702	7	83	theme	structures	1196:1205	arg1	alterations					1174:1184	the alterations	1170:1184	the alterations of glycan structures	1170:1205	To explore the molecular basis of the alterations of glycan structures, the highly sensitive next generation sequencing (NGS) technology, Illumina RNA-seq, was employed to analyze genes involved in the process of UT-A1 glycosylation using streptozotocin (STZ)-induced diabetic rat kidney.
26483702	3	84	theme	osmotic	566:572	arg1	diuresis					574:581	the osmotic diuresis	562:581	the osmotic diuresis caused by glucosuria	562:602	In diabetes, UT-A1 protein abundance, particularly the 117 kD isoform, is significantly increased corresponding to an increased urea permeability in perfused IM collecting ducts, which plays an important role in preventing the osmotic diuresis caused by glucosuria.
26483702	14	85	from	changes	2275:2281	arg1	structure					2303:2311	the UT-A1 glycan structure	2286:2311	the UT-A1 glycan structure	2286:2311	The alteration of these genes may contribute to changes in the UT-A1 glycan structure and therefore modulate kidney urea transport activity and alleviate osmotic diuresis caused by glucosuria in diabetes.
26483702	4	86	theme	structure	642:650	arg1	change					652:657	the glycan carbohydrate structure change	618:657	the glycan carbohydrate structure change	618:657	However, how the glycan carbohydrate structure change and the glycan related enzymes regulate kidney urea transport activity, particularly under diabetic condition, is largely unknown.
26483702	7	87	theme	alterations	1174:1184	arg1	basis					1161:1165	the molecular basis	1147:1165	the molecular basis of the alterations of glycan structures	1147:1205	To explore the molecular basis of the alterations of glycan structures, the highly sensitive next generation sequencing (NGS) technology, Illumina RNA-seq, was employed to analyze genes involved in the process of UT-A1 glycosylation using streptozotocin (STZ)-induced diabetic rat kidney.
26483702	0	88	theme	kidney	86:91	arg1	medulla					99:105	STZ-induced diabetic rat kidney inner medulla	61:105	STZ-induced diabetic rat kidney inner medulla	61:105	RNA-seq analysis of glycosylation related gene expression in STZ-induced diabetic rat kidney inner medulla.
26483702	2	89	with	protein	283:289	arg1	forms					314:318	two glycosylation forms	296:318	two glycosylation forms of 97 and 117 kDa	296:336	Native UT-A1 from kidney inner medulla (IM) is a heavily glycosylated protein with two glycosylation forms of 97 and 117 kDa.
26483702	7	90	dep	generation	1234:1243	arg1	technology					1262:1271	sequencing (NGS) technology	1245:1271	the highly sensitive next generation sequencing (NGS) technology	1208:1271	To explore the molecular basis of the alterations of glycan structures, the highly sensitive next generation sequencing (NGS) technology, Illumina RNA-seq, was employed to analyze genes involved in the process of UT-A1 glycosylation using streptozotocin (STZ)-induced diabetic rat kidney.
26483702	6	91	theme	glycan	1111:1116	arg1	proteins					1126:1133	β-galactoside glycan binding proteins	1097:1133	β-galactoside glycan binding proteins	1097:1133	These changes were accompanied by altered UT-A1 association with the galectin proteins, β-galactoside glycan binding proteins.
26483702	6	91	theme	glycan	1111:1116	arg1	proteins					1087:1094	the galectin proteins	1074:1094	the galectin proteins	1074:1094	These changes were accompanied by altered UT-A1 association with the galectin proteins, β-galactoside glycan binding proteins.
26483702	7	92	theme	sequencing	1245:1254	arg1	technology					1262:1271	sequencing (NGS) technology	1245:1271	the highly sensitive next generation sequencing (NGS) technology	1208:1271	To explore the molecular basis of the alterations of glycan structures, the highly sensitive next generation sequencing (NGS) technology, Illumina RNA-seq, was employed to analyze genes involved in the process of UT-A1 glycosylation using streptozotocin (STZ)-induced diabetic rat kidney.
26483702	11	93	dep	diabetes	1937:1944	arg1	only					1951:1954	only	1951:1954	only	1951:1954	CONCLUSIONS In diabetes, not only is UT-A1 protein abundance increased but the protein's glycan structure is also significantly changed.
26483702	5	94	theme	acid	937:940	arg1	branching					972:980	increased glycan branching	955:980	increased glycan branching	955:980	In this study, using sugar-specific binding lectins, we found that the carbohydrate structure of UT-A1 is changed with increased amounts of sialic acid, fucose, and increased glycan branching under diabetic conditions.
26483702	5	94	theme	acid	937:940	arg1	fucose					943:948	fucose	943:948	fucose	943:948	In this study, using sugar-specific binding lectins, we found that the carbohydrate structure of UT-A1 is changed with increased amounts of sialic acid, fucose, and increased glycan branching under diabetic conditions.
26483702	5	94	theme	acid	937:940	arg1	amounts					919:925	increased amounts	909:925	increased amounts of sialic acid, fucose, and increased glycan branching	909:980	In this study, using sugar-specific binding lectins, we found that the carbohydrate structure of UT-A1 is changed with increased amounts of sialic acid, fucose, and increased glycan branching under diabetic conditions.
26483702	5	94	theme	acid	937:940	arg1	acid					937:940	sialic acid	930:940	sialic acid	930:940	In this study, using sugar-specific binding lectins, we found that the carbohydrate structure of UT-A1 is changed with increased amounts of sialic acid, fucose, and increased glycan branching under diabetic conditions.
26483702	6	95	theme	altered	1043:1049	arg1	association					1057:1067	altered UT-A1 association	1043:1067	altered UT-A1 association with the galectin proteins, β-galactoside glycan binding proteins	1043:1133	These changes were accompanied by altered UT-A1 association with the galectin proteins, β-galactoside glycan binding proteins.
26483702	9	96	dep	genes	1657:1661	arg1	galectin-3					1742:1751	glycan binding protein galectin-3	1719:1751	glycan binding protein galectin-3	1719:1751	These genes include the glycosyltransferase genes Mgat4a, the sialylation enzymes St3gal1 and St3gal4 and glycan binding protein galectin-3, -5, -8, and -9.
26483702	9	96	dep	genes	1657:1661	arg1	genes					1657:1661	the glycosyltransferase genes Mgat4a, the sialylation enzymes St3gal1 and St3gal4 and glycan binding protein galectin-3	1633:1751	the glycosyltransferase genes Mgat4a, the sialylation enzymes St3gal1 and St3gal4 and glycan binding protein galectin-3	1633:1751	These genes include the glycosyltransferase genes Mgat4a, the sialylation enzymes St3gal1 and St3gal4 and glycan binding protein galectin-3, -5, -8, and -9.
26483702	9	96	dep	genes	1657:1661	arg1	St3gal1					1695:1701	St3gal1	1695:1701	St3gal1	1695:1701	These genes include the glycosyltransferase genes Mgat4a, the sialylation enzymes St3gal1 and St3gal4 and glycan binding protein galectin-3, -5, -8, and -9.
26483702	9	96	dep	genes	1657:1661	arg1	Mgat4a					1663:1668	Mgat4a	1663:1668	Mgat4a	1663:1668	These genes include the glycosyltransferase genes Mgat4a, the sialylation enzymes St3gal1 and St3gal4 and glycan binding protein galectin-3, -5, -8, and -9.
26483702	9	96	dep	genes	1657:1661	arg1	St3gal4					1707:1713	St3gal4	1707:1713	St3gal4	1707:1713	These genes include the glycosyltransferase genes Mgat4a, the sialylation enzymes St3gal1 and St3gal4 and glycan binding protein galectin-3, -5, -8, and -9.
26483702	9	96	dep	genes	1657:1661	arg1	enzymes					1687:1693	the sialylation enzymes St3gal1 and St3gal4	1671:1713	the sialylation enzymes St3gal1 and St3gal4	1671:1713	These genes include the glycosyltransferase genes Mgat4a, the sialylation enzymes St3gal1 and St3gal4 and glycan binding protein galectin-3, -5, -8, and -9.
26483702	3	97	theme	UT-A1	352:356	arg1	abundance					366:374	UT-A1 protein abundance	352:374	UT-A1 protein abundance	352:374	In diabetes, UT-A1 protein abundance, particularly the 117 kD isoform, is significantly increased corresponding to an increased urea permeability in perfused IM collecting ducts, which plays an important role in preventing the osmotic diuresis caused by glucosuria.
26483702	3	97	theme	UT-A1	352:356	arg1	isoform					401:407	the 117 kD isoform	390:407	particularly the 117 kD isoform	377:407	In diabetes, UT-A1 protein abundance, particularly the 117 kD isoform, is significantly increased corresponding to an increased urea permeability in perfused IM collecting ducts, which plays an important role in preventing the osmotic diuresis caused by glucosuria.
26483702	8	98	theme	number	1525:1530	arg1	expression					1509:1518	expression	1509:1518	expression of a number of important glycosylation related genes	1509:1571	Differential gene expression analysis combining with quantitative PCR revealed that expression of a number of important glycosylation related genes were changed under diabetic conditions.
26483702	4	99	theme	kidney	699:704	arg1	transport					711:719	kidney urea transport	699:719	kidney urea transport activity	699:728	However, how the glycan carbohydrate structure change and the glycan related enzymes regulate kidney urea transport activity, particularly under diabetic condition, is largely unknown.
26483702	3	100	theme	117	394:396	arg1	kD					398:399	kD	398:399	kD	398:399	In diabetes, UT-A1 protein abundance, particularly the 117 kD isoform, is significantly increased corresponding to an increased urea permeability in perfused IM collecting ducts, which plays an important role in preventing the osmotic diuresis caused by glucosuria.
26483702	0	101	theme	glycosylation	20:32	arg1	analysis					8:15	RNA-seq analysis	0:15	RNA-seq analysis of glycosylation	0:32	RNA-seq analysis of glycosylation related gene expression in STZ-induced diabetic rat kidney inner medulla.
26483702	5	102	theme	increased	909:917	arg1	branching					972:980	increased glycan branching	955:980	increased glycan branching	955:980	In this study, using sugar-specific binding lectins, we found that the carbohydrate structure of UT-A1 is changed with increased amounts of sialic acid, fucose, and increased glycan branching under diabetic conditions.
26483702	5	102	theme	increased	909:917	arg1	fucose					943:948	fucose	943:948	fucose	943:948	In this study, using sugar-specific binding lectins, we found that the carbohydrate structure of UT-A1 is changed with increased amounts of sialic acid, fucose, and increased glycan branching under diabetic conditions.
26483702	5	102	theme	increased	909:917	arg1	amounts					919:925	increased amounts	909:925	increased amounts of sialic acid, fucose, and increased glycan branching	909:980	In this study, using sugar-specific binding lectins, we found that the carbohydrate structure of UT-A1 is changed with increased amounts of sialic acid, fucose, and increased glycan branching under diabetic conditions.
26483702	5	102	theme	increased	909:917	arg1	acid					937:940	sialic acid	930:940	sialic acid	930:940	In this study, using sugar-specific binding lectins, we found that the carbohydrate structure of UT-A1 is changed with increased amounts of sialic acid, fucose, and increased glycan branching under diabetic conditions.
26483702	8	103	theme	important	1535:1543	arg1	genes					1567:1571	important glycosylation related genes	1535:1571	important glycosylation related genes	1535:1571	Differential gene expression analysis combining with quantitative PCR revealed that expression of a number of important glycosylation related genes were changed under diabetic conditions.
26483702	10	104	theme	glycosyltransferase	1827:1845	arg1	Mgat1					1853:1857	Mgat1	1853:1857	Mgat1	1853:1857	In contrast, although highly expressed in kidney IM, the glycosyltransferase genes Mgat1, Mgat2, and fucosyltransferase Fut8, did not show any changes.
26483702	10	104	theme	glycosyltransferase	1827:1845	arg1	IM					1819:1820	kidney IM	1812:1820	kidney IM	1812:1820	In contrast, although highly expressed in kidney IM, the glycosyltransferase genes Mgat1, Mgat2, and fucosyltransferase Fut8, did not show any changes.
26483702	10	104	theme	glycosyltransferase	1827:1845	arg1	Fut8					1890:1893	fucosyltransferase Fut8	1871:1893	fucosyltransferase Fut8	1871:1893	In contrast, although highly expressed in kidney IM, the glycosyltransferase genes Mgat1, Mgat2, and fucosyltransferase Fut8, did not show any changes.
26483702	10	104	theme	glycosyltransferase	1827:1845	arg1	genes					1847:1851	the glycosyltransferase genes Mgat1, Mgat2, and fucosyltransferase Fut8	1823:1893	the glycosyltransferase genes Mgat1, Mgat2, and fucosyltransferase Fut8	1823:1893	In contrast, although highly expressed in kidney IM, the glycosyltransferase genes Mgat1, Mgat2, and fucosyltransferase Fut8, did not show any changes.
26483702	10	104	theme	glycosyltransferase	1827:1845	arg1	Mgat2					1860:1864	Mgat2	1860:1864	Mgat2	1860:1864	In contrast, although highly expressed in kidney IM, the glycosyltransferase genes Mgat1, Mgat2, and fucosyltransferase Fut8, did not show any changes.
26483702	4	105	theme	transport	711:719	arg1	activity					721:728	kidney urea transport activity	699:728	kidney urea transport activity	699:728	However, how the glycan carbohydrate structure change and the glycan related enzymes regulate kidney urea transport activity, particularly under diabetic condition, is largely unknown.
26483702	0	106	theme	gene	42:45	arg1	expression					47:56	gene expression	42:56	gene expression in STZ-induced diabetic rat kidney inner medulla	42:105	RNA-seq analysis of glycosylation related gene expression in STZ-induced diabetic rat kidney inner medulla.
26483702	13	107	theme	diabetic	2206:2213	arg1	conditions					2215:2224	diabetic conditions	2206:2224	diabetic conditions	2206:2224	Consistently, a number of crucial glycosylation related genes are changed under diabetic conditions.
26483702	5	108	theme	glycan	965:970	arg1	branching					972:980	increased glycan branching	955:980	increased glycan branching	955:980	In this study, using sugar-specific binding lectins, we found that the carbohydrate structure of UT-A1 is changed with increased amounts of sialic acid, fucose, and increased glycan branching under diabetic conditions.
26483702	8	109	theme	related	1559:1565	arg1	genes					1567:1571	important glycosylation related genes	1535:1571	important glycosylation related genes	1535:1571	Differential gene expression analysis combining with quantitative PCR revealed that expression of a number of important glycosylation related genes were changed under diabetic conditions.
26483702	13	110	theme	glycosylation	2160:2172	arg1	genes					2182:2186	crucial glycosylation related genes	2152:2186	crucial glycosylation related genes	2152:2186	Consistently, a number of crucial glycosylation related genes are changed under diabetic conditions.
26483702	6	111	theme	β-galactoside	1097:1109	arg1	proteins					1126:1133	β-galactoside glycan binding proteins	1097:1133	β-galactoside glycan binding proteins	1097:1133	These changes were accompanied by altered UT-A1 association with the galectin proteins, β-galactoside glycan binding proteins.
26483702	6	111	theme	β-galactoside	1097:1109	arg1	proteins					1087:1094	the galectin proteins	1074:1094	the galectin proteins	1074:1094	These changes were accompanied by altered UT-A1 association with the galectin proteins, β-galactoside glycan binding proteins.
26483702	2	112	theme	kDa	334:336	arg1	forms					314:318	two glycosylation forms	296:318	two glycosylation forms of 97 and 117 kDa	296:336	Native UT-A1 from kidney inner medulla (IM) is a heavily glycosylated protein with two glycosylation forms of 97 and 117 kDa.
26483702	3	113	theme	increased	457:465	arg1	permeability					472:483	an increased urea permeability	454:483	an increased urea permeability in perfused IM collecting ducts, which plays an important role in preventing the osmotic diuresis caused by glucosuria	454:602	In diabetes, UT-A1 protein abundance, particularly the 117 kD isoform, is significantly increased corresponding to an increased urea permeability in perfused IM collecting ducts, which plays an important role in preventing the osmotic diuresis caused by glucosuria.
26483702	13	114	theme	genes	2182:2186	arg1	number					2142:2147	a number	2140:2147	a number of crucial glycosylation related genes	2140:2186	Consistently, a number of crucial glycosylation related genes are changed under diabetic conditions.
26483702	5	115	theme	fucose	943:948	arg1	branching					972:980	increased glycan branching	955:980	increased glycan branching	955:980	In this study, using sugar-specific binding lectins, we found that the carbohydrate structure of UT-A1 is changed with increased amounts of sialic acid, fucose, and increased glycan branching under diabetic conditions.
26483702	5	115	theme	fucose	943:948	arg1	fucose					943:948	fucose	943:948	fucose	943:948	In this study, using sugar-specific binding lectins, we found that the carbohydrate structure of UT-A1 is changed with increased amounts of sialic acid, fucose, and increased glycan branching under diabetic conditions.
26483702	5	115	theme	fucose	943:948	arg1	amounts					919:925	increased amounts	909:925	increased amounts of sialic acid, fucose, and increased glycan branching	909:980	In this study, using sugar-specific binding lectins, we found that the carbohydrate structure of UT-A1 is changed with increased amounts of sialic acid, fucose, and increased glycan branching under diabetic conditions.
26483702	5	115	theme	fucose	943:948	arg1	acid					937:940	sialic acid	930:940	sialic acid	930:940	In this study, using sugar-specific binding lectins, we found that the carbohydrate structure of UT-A1 is changed with increased amounts of sialic acid, fucose, and increased glycan branching under diabetic conditions.
26483702	2	116	theme	glycosylation	300:312	arg1	forms					314:318	two glycosylation forms	296:318	two glycosylation forms of 97 and 117 kDa	296:336	Native UT-A1 from kidney inner medulla (IM) is a heavily glycosylated protein with two glycosylation forms of 97 and 117 kDa.
26483702	4	117	dep	unknown	781:787	arg1	regulate					690:697	regulate	690:697	regulate kidney urea transport activity	690:728	However, how the glycan carbohydrate structure change and the glycan related enzymes regulate kidney urea transport activity, particularly under diabetic condition, is largely unknown.
26483702	7	118	theme	sensitive	1219:1227	arg1	RNA-seq					1283:1289	Illumina RNA-seq	1274:1289	Illumina RNA-seq	1274:1289	To explore the molecular basis of the alterations of glycan structures, the highly sensitive next generation sequencing (NGS) technology, Illumina RNA-seq, was employed to analyze genes involved in the process of UT-A1 glycosylation using streptozotocin (STZ)-induced diabetic rat kidney.
26483702	7	118	theme	sensitive	1219:1227	arg1	generation					1234:1243	the highly sensitive next generation	1208:1243	the highly sensitive next generation sequencing (NGS) technology	1208:1271	To explore the molecular basis of the alterations of glycan structures, the highly sensitive next generation sequencing (NGS) technology, Illumina RNA-seq, was employed to analyze genes involved in the process of UT-A1 glycosylation using streptozotocin (STZ)-induced diabetic rat kidney.
26483702	14	119	theme	osmotic	2381:2387	arg1	diuresis					2389:2396	osmotic diuresis	2381:2396	osmotic diuresis caused by glucosuria in diabetes	2381:2429	The alteration of these genes may contribute to changes in the UT-A1 glycan structure and therefore modulate kidney urea transport activity and alleviate osmotic diuresis caused by glucosuria in diabetes.
26483702	3	120	theme	perfused	488:495	arg1	ducts					511:515	perfused IM collecting ducts	488:515	perfused IM collecting ducts	488:515	In diabetes, UT-A1 protein abundance, particularly the 117 kD isoform, is significantly increased corresponding to an increased urea permeability in perfused IM collecting ducts, which plays an important role in preventing the osmotic diuresis caused by glucosuria.
27028263	0	0	theme	inside	109:114	arg1	Cells					123:127	inside Immune Cells	109:127	inside Immune Cells	109:127	Broad-Spectrum Antimicrobial Star Polycarbonates Functionalized with Mannose for Targeting Bacteria Residing inside Immune Cells.
27028263	2	1	theme	precise	645:651	arg1	control					653:659	precise control	645:659	precise control over the molecular composition	645:690	The distributions and compositions of pendent benzyl chloride and protected mannose group (ipman) units are facilely modulated by varying the polymerization sequence and feed ratio of the monomers, allowing precise control over the molecular composition, and the resulting polymers have narrow molecular weight distribution.
27028263	3	2	with	polymers	866:873	arg1	compositions					890:901	optimized compositions	880:901	optimized compositions of cationic and mannose groups in block	880:941	After deprotection of ipman groups and quaternization with various N,N-dimethylalkylamines, these star polymers with optimized compositions of cationic and mannose groups in block and random forms exhibit strong bactericidal activity and low hemolysis.
27028263	1	3	theme	star-shaped	157:167	arg1	polycarbonates					169:182	star-shaped polycarbonates	157:182	star-shaped polycarbonates	157:182	In this study, a series of star-shaped polycarbonates are synthesized by metal-free organocatalytic ring-opening polymerization of benzyl chloride (BnCl) and mannose-functionalized cyclic carbonate monomers (MTC-BnCl and MTC-ipman) with heptakis-(2,3-di-O-acetyl)-β-cyclodextrin (DA-β-CD) as macroinitiator.
27028263	3	4	theme	random	947:952	arg1	forms					954:958	random forms	947:958	random forms	947:958	After deprotection of ipman groups and quaternization with various N,N-dimethylalkylamines, these star polymers with optimized compositions of cationic and mannose groups in block and random forms exhibit strong bactericidal activity and low hemolysis.
27028263	5	5	theme	antimicrobial	1319:1331	arg1	agents					1333:1338	antimicrobial agents	1319:1338	antimicrobial agents against diseases such as tuberculosis, which is caused by intracellular bacteria	1319:1419	Taken together, the materials designed in this study have potential use as antimicrobial agents against diseases such as tuberculosis, which is caused by intracellular bacteria.
27028263	2	6	theme	ipman	529:533	arg1	units					536:540	protected mannose group (ipman) units	504:540	protected mannose group (ipman) units	504:540	The distributions and compositions of pendent benzyl chloride and protected mannose group (ipman) units are facilely modulated by varying the polymerization sequence and feed ratio of the monomers, allowing precise control over the molecular composition, and the resulting polymers have narrow molecular weight distribution.
27028263	1	7	theme	2,3-di-O-acetyl	377:391	arg1	DA-β-CD					410:416	DA-β-CD	410:416	DA-β-CD	410:416	In this study, a series of star-shaped polycarbonates are synthesized by metal-free organocatalytic ring-opening polymerization of benzyl chloride (BnCl) and mannose-functionalized cyclic carbonate monomers (MTC-BnCl and MTC-ipman) with heptakis-(2,3-di-O-acetyl)-β-cyclodextrin (DA-β-CD) as macroinitiator.
27028263	1	7	theme	2,3-di-O-acetyl	377:391	arg1	-β-cyclodextrin					393:407	heptakis-(2,3-di-O-acetyl)-β-cyclodextrin	367:407	heptakis-(2,3-di-O-acetyl)-β-cyclodextrin (DA-β-CD)	367:417	In this study, a series of star-shaped polycarbonates are synthesized by metal-free organocatalytic ring-opening polymerization of benzyl chloride (BnCl) and mannose-functionalized cyclic carbonate monomers (MTC-BnCl and MTC-ipman) with heptakis-(2,3-di-O-acetyl)-β-cyclodextrin (DA-β-CD) as macroinitiator.
27028263	1	8	theme	polycarbonates	169:182	arg1	macroinitiator					422:435	macroinitiator	422:435	macroinitiator	422:435	In this study, a series of star-shaped polycarbonates are synthesized by metal-free organocatalytic ring-opening polymerization of benzyl chloride (BnCl) and mannose-functionalized cyclic carbonate monomers (MTC-BnCl and MTC-ipman) with heptakis-(2,3-di-O-acetyl)-β-cyclodextrin (DA-β-CD) as macroinitiator.
27028263	1	8	theme	polycarbonates	169:182	arg1	series					147:152	a series	145:152	a series of star-shaped polycarbonates	145:182	In this study, a series of star-shaped polycarbonates are synthesized by metal-free organocatalytic ring-opening polymerization of benzyl chloride (BnCl) and mannose-functionalized cyclic carbonate monomers (MTC-BnCl and MTC-ipman) with heptakis-(2,3-di-O-acetyl)-β-cyclodextrin (DA-β-CD) as macroinitiator.
27028263	5	9	theme	intracellular	1398:1410	arg1	bacteria					1412:1419	intracellular bacteria	1398:1419	intracellular bacteria	1398:1419	Taken together, the materials designed in this study have potential use as antimicrobial agents against diseases such as tuberculosis, which is caused by intracellular bacteria.
27028263	1	10	dep	monomers	328:335	arg1	MTC-ipman					351:359	MTC-ipman	351:359	MTC-ipman	351:359	In this study, a series of star-shaped polycarbonates are synthesized by metal-free organocatalytic ring-opening polymerization of benzyl chloride (BnCl) and mannose-functionalized cyclic carbonate monomers (MTC-BnCl and MTC-ipman) with heptakis-(2,3-di-O-acetyl)-β-cyclodextrin (DA-β-CD) as macroinitiator.
27028263	1	10	dep	monomers	328:335	arg1	MTC-BnCl					338:345	MTC-BnCl	338:345	MTC-BnCl	338:345	In this study, a series of star-shaped polycarbonates are synthesized by metal-free organocatalytic ring-opening polymerization of benzyl chloride (BnCl) and mannose-functionalized cyclic carbonate monomers (MTC-BnCl and MTC-ipman) with heptakis-(2,3-di-O-acetyl)-β-cyclodextrin (DA-β-CD) as macroinitiator.
27028263	1	10	dep	monomers	328:335	arg1	monomers					328:335	benzyl chloride (BnCl) and mannose-functionalized cyclic carbonate monomers	261:335	benzyl chloride (BnCl) and mannose-functionalized cyclic carbonate monomers (MTC-BnCl and MTC-ipman)	261:360	In this study, a series of star-shaped polycarbonates are synthesized by metal-free organocatalytic ring-opening polymerization of benzyl chloride (BnCl) and mannose-functionalized cyclic carbonate monomers (MTC-BnCl and MTC-ipman) with heptakis-(2,3-di-O-acetyl)-β-cyclodextrin (DA-β-CD) as macroinitiator.
27028263	5	11	theme	potential	1302:1310	arg1	use					1312:1314	potential use	1302:1314	potential use as antimicrobial agents against diseases such as tuberculosis, which is caused by intracellular bacteria	1302:1419	Taken together, the materials designed in this study have potential use as antimicrobial agents against diseases such as tuberculosis, which is caused by intracellular bacteria.
27028263	1	12	theme	mannose-functionalized	288:309	arg1	carbonate					318:326	mannose-functionalized cyclic carbonate	288:326	mannose-functionalized cyclic carbonate	288:326	In this study, a series of star-shaped polycarbonates are synthesized by metal-free organocatalytic ring-opening polymerization of benzyl chloride (BnCl) and mannose-functionalized cyclic carbonate monomers (MTC-BnCl and MTC-ipman) with heptakis-(2,3-di-O-acetyl)-β-cyclodextrin (DA-β-CD) as macroinitiator.
27028263	0	13	theme	Immune	116:121	arg1	Cells					123:127	inside Immune Cells	109:127	inside Immune Cells	109:127	Broad-Spectrum Antimicrobial Star Polycarbonates Functionalized with Mannose for Targeting Bacteria Residing inside Immune Cells.
27028263	2	14	theme	monomers	626:633	arg1	sequence					595:602	polymerization sequence	580:602	polymerization sequence	580:602	The distributions and compositions of pendent benzyl chloride and protected mannose group (ipman) units are facilely modulated by varying the polymerization sequence and feed ratio of the monomers, allowing precise control over the molecular composition, and the resulting polymers have narrow molecular weight distribution.
27028263	2	14	theme	monomers	626:633	arg1	ratio					613:617	feed ratio	608:617	feed ratio	608:617	The distributions and compositions of pendent benzyl chloride and protected mannose group (ipman) units are facilely modulated by varying the polymerization sequence and feed ratio of the monomers, allowing precise control over the molecular composition, and the resulting polymers have narrow molecular weight distribution.
27028263	3	15	from	block	937:941	arg1	compositions					890:901	optimized compositions	880:901	optimized compositions of cationic and mannose groups in block	880:941	After deprotection of ipman groups and quaternization with various N,N-dimethylalkylamines, these star polymers with optimized compositions of cationic and mannose groups in block and random forms exhibit strong bactericidal activity and low hemolysis.
27028263	3	16	theme	strong	968:973	arg1	activity					988:995	strong bactericidal activity	968:995	strong bactericidal activity	968:995	After deprotection of ipman groups and quaternization with various N,N-dimethylalkylamines, these star polymers with optimized compositions of cationic and mannose groups in block and random forms exhibit strong bactericidal activity and low hemolysis.
27028263	1	17	theme	cyclic	311:316	arg1	carbonate					318:326	mannose-functionalized cyclic carbonate	288:326	mannose-functionalized cyclic carbonate	288:326	In this study, a series of star-shaped polycarbonates are synthesized by metal-free organocatalytic ring-opening polymerization of benzyl chloride (BnCl) and mannose-functionalized cyclic carbonate monomers (MTC-BnCl and MTC-ipman) with heptakis-(2,3-di-O-acetyl)-β-cyclodextrin (DA-β-CD) as macroinitiator.
27028263	3	18	theme	ipman	785:789	arg1	groups					791:796	ipman groups	785:796	ipman groups	785:796	After deprotection of ipman groups and quaternization with various N,N-dimethylalkylamines, these star polymers with optimized compositions of cationic and mannose groups in block and random forms exhibit strong bactericidal activity and low hemolysis.
27028263	2	19	theme	molecular	670:678	arg1	composition					680:690	the molecular composition	666:690	the molecular composition	666:690	The distributions and compositions of pendent benzyl chloride and protected mannose group (ipman) units are facilely modulated by varying the polymerization sequence and feed ratio of the monomers, allowing precise control over the molecular composition, and the resulting polymers have narrow molecular weight distribution.
27028263	3	20	with	N-dimethylalkylamines	832:852	arg1	compositions					890:901	optimized compositions	880:901	optimized compositions of cationic and mannose groups in block	880:941	After deprotection of ipman groups and quaternization with various N,N-dimethylalkylamines, these star polymers with optimized compositions of cationic and mannose groups in block and random forms exhibit strong bactericidal activity and low hemolysis.
27028263	3	21	theme	bactericidal	975:986	arg1	activity					988:995	strong bactericidal activity	968:995	strong bactericidal activity	968:995	After deprotection of ipman groups and quaternization with various N,N-dimethylalkylamines, these star polymers with optimized compositions of cationic and mannose groups in block and random forms exhibit strong bactericidal activity and low hemolysis.
27028263	2	22	theme	protected	504:512	arg1	units					536:540	protected mannose group (ipman) units	504:540	protected mannose group (ipman) units	504:540	The distributions and compositions of pendent benzyl chloride and protected mannose group (ipman) units are facilely modulated by varying the polymerization sequence and feed ratio of the monomers, allowing precise control over the molecular composition, and the resulting polymers have narrow molecular weight distribution.
27028263	2	23	theme	narrow	725:730	arg1	distribution					749:760	narrow molecular weight distribution	725:760	narrow molecular weight distribution	725:760	The distributions and compositions of pendent benzyl chloride and protected mannose group (ipman) units are facilely modulated by varying the polymerization sequence and feed ratio of the monomers, allowing precise control over the molecular composition, and the resulting polymers have narrow molecular weight distribution.
27028263	2	24	theme	chloride	491:498	arg1	compositions					460:471	compositions	460:471	compositions	460:471	The distributions and compositions of pendent benzyl chloride and protected mannose group (ipman) units are facilely modulated by varying the polymerization sequence and feed ratio of the monomers, allowing precise control over the molecular composition, and the resulting polymers have narrow molecular weight distribution.
27028263	2	24	theme	chloride	491:498	arg1	distributions					442:454	distributions	442:454	distributions	442:454	The distributions and compositions of pendent benzyl chloride and protected mannose group (ipman) units are facilely modulated by varying the polymerization sequence and feed ratio of the monomers, allowing precise control over the molecular composition, and the resulting polymers have narrow molecular weight distribution.
27028263	0	25	theme	Antimicrobial	15:27	arg1	Polycarbonates					34:47	Broad-Spectrum Antimicrobial Star Polycarbonates	0:47	Broad-Spectrum Antimicrobial Star Polycarbonates	0:47	Broad-Spectrum Antimicrobial Star Polycarbonates Functionalized with Mannose for Targeting Bacteria Residing inside Immune Cells.
27028263	0	26	theme	Broad-Spectrum	0:13	arg1	Polycarbonates					34:47	Broad-Spectrum Antimicrobial Star Polycarbonates	0:47	Broad-Spectrum Antimicrobial Star Polycarbonates	0:47	Broad-Spectrum Antimicrobial Star Polycarbonates Functionalized with Mannose for Targeting Bacteria Residing inside Immune Cells.
27028263	1	27	theme	carbonate	318:326	arg1	MTC-ipman					351:359	MTC-ipman	351:359	MTC-ipman	351:359	In this study, a series of star-shaped polycarbonates are synthesized by metal-free organocatalytic ring-opening polymerization of benzyl chloride (BnCl) and mannose-functionalized cyclic carbonate monomers (MTC-BnCl and MTC-ipman) with heptakis-(2,3-di-O-acetyl)-β-cyclodextrin (DA-β-CD) as macroinitiator.
27028263	1	27	theme	carbonate	318:326	arg1	MTC-BnCl					338:345	MTC-BnCl	338:345	MTC-BnCl	338:345	In this study, a series of star-shaped polycarbonates are synthesized by metal-free organocatalytic ring-opening polymerization of benzyl chloride (BnCl) and mannose-functionalized cyclic carbonate monomers (MTC-BnCl and MTC-ipman) with heptakis-(2,3-di-O-acetyl)-β-cyclodextrin (DA-β-CD) as macroinitiator.
27028263	1	27	theme	carbonate	318:326	arg1	monomers					328:335	benzyl chloride (BnCl) and mannose-functionalized cyclic carbonate monomers	261:335	benzyl chloride (BnCl) and mannose-functionalized cyclic carbonate monomers (MTC-BnCl and MTC-ipman)	261:360	In this study, a series of star-shaped polycarbonates are synthesized by metal-free organocatalytic ring-opening polymerization of benzyl chloride (BnCl) and mannose-functionalized cyclic carbonate monomers (MTC-BnCl and MTC-ipman) with heptakis-(2,3-di-O-acetyl)-β-cyclodextrin (DA-β-CD) as macroinitiator.
27028263	3	28	with	quaternization	802:815	arg1	N					830:830	various N	822:830	various N	822:830	After deprotection of ipman groups and quaternization with various N,N-dimethylalkylamines, these star polymers with optimized compositions of cationic and mannose groups in block and random forms exhibit strong bactericidal activity and low hemolysis.
27028263	3	29	theme	low	1001:1003	arg1	hemolysis					1005:1013	low hemolysis	1001:1013	low hemolysis	1001:1013	After deprotection of ipman groups and quaternization with various N,N-dimethylalkylamines, these star polymers with optimized compositions of cationic and mannose groups in block and random forms exhibit strong bactericidal activity and low hemolysis.
27028263	1	30	theme	metal-free	203:212	arg1	polymerization					243:256	metal-free organocatalytic ring-opening polymerization	203:256	metal-free organocatalytic ring-opening polymerization of benzyl chloride (BnCl) and mannose-functionalized cyclic carbonate monomers (MTC-BnCl and MTC-ipman) with heptakis-(2,3-di-O-acetyl)-β-cyclodextrin (DA-β-CD)	203:417	In this study, a series of star-shaped polycarbonates are synthesized by metal-free organocatalytic ring-opening polymerization of benzyl chloride (BnCl) and mannose-functionalized cyclic carbonate monomers (MTC-BnCl and MTC-ipman) with heptakis-(2,3-di-O-acetyl)-β-cyclodextrin (DA-β-CD) as macroinitiator.
27028263	2	31	theme	benzyl	484:489	arg1	chloride					491:498	pendent benzyl chloride	476:498	pendent benzyl chloride	476:498	The distributions and compositions of pendent benzyl chloride and protected mannose group (ipman) units are facilely modulated by varying the polymerization sequence and feed ratio of the monomers, allowing precise control over the molecular composition, and the resulting polymers have narrow molecular weight distribution.
27028263	1	32	theme	monomers	328:335	arg1	polymerization					243:256	metal-free organocatalytic ring-opening polymerization	203:256	metal-free organocatalytic ring-opening polymerization of benzyl chloride (BnCl) and mannose-functionalized cyclic carbonate monomers (MTC-BnCl and MTC-ipman) with heptakis-(2,3-di-O-acetyl)-β-cyclodextrin (DA-β-CD)	203:417	In this study, a series of star-shaped polycarbonates are synthesized by metal-free organocatalytic ring-opening polymerization of benzyl chloride (BnCl) and mannose-functionalized cyclic carbonate monomers (MTC-BnCl and MTC-ipman) with heptakis-(2,3-di-O-acetyl)-β-cyclodextrin (DA-β-CD) as macroinitiator.
27028263	1	33	with	polymerization	243:256	arg1	DA-β-CD					410:416	DA-β-CD	410:416	DA-β-CD	410:416	In this study, a series of star-shaped polycarbonates are synthesized by metal-free organocatalytic ring-opening polymerization of benzyl chloride (BnCl) and mannose-functionalized cyclic carbonate monomers (MTC-BnCl and MTC-ipman) with heptakis-(2,3-di-O-acetyl)-β-cyclodextrin (DA-β-CD) as macroinitiator.
27028263	1	33	with	polymerization	243:256	arg1	-β-cyclodextrin					393:407	heptakis-(2,3-di-O-acetyl)-β-cyclodextrin	367:407	heptakis-(2,3-di-O-acetyl)-β-cyclodextrin (DA-β-CD)	367:417	In this study, a series of star-shaped polycarbonates are synthesized by metal-free organocatalytic ring-opening polymerization of benzyl chloride (BnCl) and mannose-functionalized cyclic carbonate monomers (MTC-BnCl and MTC-ipman) with heptakis-(2,3-di-O-acetyl)-β-cyclodextrin (DA-β-CD) as macroinitiator.
27028263	2	34	theme	resulting	701:709	arg1	polymers					711:718	the resulting polymers	697:718	the resulting polymers	697:718	The distributions and compositions of pendent benzyl chloride and protected mannose group (ipman) units are facilely modulated by varying the polymerization sequence and feed ratio of the monomers, allowing precise control over the molecular composition, and the resulting polymers have narrow molecular weight distribution.
27028263	3	35	theme	optimized	880:888	arg1	compositions					890:901	optimized compositions	880:901	optimized compositions of cationic and mannose groups in block	880:941	After deprotection of ipman groups and quaternization with various N,N-dimethylalkylamines, these star polymers with optimized compositions of cationic and mannose groups in block and random forms exhibit strong bactericidal activity and low hemolysis.
27028263	4	36	theme	effective	1228:1236	arg1	dose					1238:1241	the effective dose	1224:1241	the effective dose	1224:1241	Furthermore, the optimal mannose-functionalized polymer demonstrates mannose receptor-mediated intracellular bactericidal activity against BCG mycobacteria without inducing cytotoxicity on mammalian cells at the effective dose.
27028263	1	37	theme	organocatalytic	214:228	arg1	polymerization					243:256	metal-free organocatalytic ring-opening polymerization	203:256	metal-free organocatalytic ring-opening polymerization of benzyl chloride (BnCl) and mannose-functionalized cyclic carbonate monomers (MTC-BnCl and MTC-ipman) with heptakis-(2,3-di-O-acetyl)-β-cyclodextrin (DA-β-CD)	203:417	In this study, a series of star-shaped polycarbonates are synthesized by metal-free organocatalytic ring-opening polymerization of benzyl chloride (BnCl) and mannose-functionalized cyclic carbonate monomers (MTC-BnCl and MTC-ipman) with heptakis-(2,3-di-O-acetyl)-β-cyclodextrin (DA-β-CD) as macroinitiator.
27028263	0	38	theme	Star	29:32	arg1	Polycarbonates					34:47	Broad-Spectrum Antimicrobial Star Polycarbonates	0:47	Broad-Spectrum Antimicrobial Star Polycarbonates	0:47	Broad-Spectrum Antimicrobial Star Polycarbonates Functionalized with Mannose for Targeting Bacteria Residing inside Immune Cells.
27028263	3	39	theme	groups	791:796	arg1	quaternization					802:815	quaternization	802:815	quaternization with various N	802:830	After deprotection of ipman groups and quaternization with various N,N-dimethylalkylamines, these star polymers with optimized compositions of cationic and mannose groups in block and random forms exhibit strong bactericidal activity and low hemolysis.
27028263	3	39	theme	groups	791:796	arg1	deprotection					769:780	deprotection	769:780	deprotection of ipman groups	769:796	After deprotection of ipman groups and quaternization with various N,N-dimethylalkylamines, these star polymers with optimized compositions of cationic and mannose groups in block and random forms exhibit strong bactericidal activity and low hemolysis.
27028263	4	40	theme	mammalian	1205:1213	arg1	cells					1215:1219	mammalian cells	1205:1219	mammalian cells at the effective dose	1205:1241	Furthermore, the optimal mannose-functionalized polymer demonstrates mannose receptor-mediated intracellular bactericidal activity against BCG mycobacteria without inducing cytotoxicity on mammalian cells at the effective dose.
27028263	3	41	from	groups	927:932	arg1	block					937:941	block	937:941	block	937:941	After deprotection of ipman groups and quaternization with various N,N-dimethylalkylamines, these star polymers with optimized compositions of cationic and mannose groups in block and random forms exhibit strong bactericidal activity and low hemolysis.
27028263	1	42	theme	ring-opening	230:241	arg1	polymerization					243:256	metal-free organocatalytic ring-opening polymerization	203:256	metal-free organocatalytic ring-opening polymerization of benzyl chloride (BnCl) and mannose-functionalized cyclic carbonate monomers (MTC-BnCl and MTC-ipman) with heptakis-(2,3-di-O-acetyl)-β-cyclodextrin (DA-β-CD)	203:417	In this study, a series of star-shaped polycarbonates are synthesized by metal-free organocatalytic ring-opening polymerization of benzyl chloride (BnCl) and mannose-functionalized cyclic carbonate monomers (MTC-BnCl and MTC-ipman) with heptakis-(2,3-di-O-acetyl)-β-cyclodextrin (DA-β-CD) as macroinitiator.
27028263	4	43	theme	receptor-mediated	1093:1109	arg1	activity					1138:1145	mannose receptor-mediated intracellular bactericidal activity	1085:1145	mannose receptor-mediated intracellular bactericidal activity against BCG mycobacteria	1085:1170	Furthermore, the optimal mannose-functionalized polymer demonstrates mannose receptor-mediated intracellular bactericidal activity against BCG mycobacteria without inducing cytotoxicity on mammalian cells at the effective dose.
27028263	2	44	theme	polymerization	580:593	arg1	sequence					595:602	polymerization sequence	580:602	polymerization sequence	580:602	The distributions and compositions of pendent benzyl chloride and protected mannose group (ipman) units are facilely modulated by varying the polymerization sequence and feed ratio of the monomers, allowing precise control over the molecular composition, and the resulting polymers have narrow molecular weight distribution.
27028263	4	45	theme	bactericidal	1125:1136	arg1	activity					1138:1145	mannose receptor-mediated intracellular bactericidal activity	1085:1145	mannose receptor-mediated intracellular bactericidal activity against BCG mycobacteria	1085:1170	Furthermore, the optimal mannose-functionalized polymer demonstrates mannose receptor-mediated intracellular bactericidal activity against BCG mycobacteria without inducing cytotoxicity on mammalian cells at the effective dose.
27028263	4	46	from	dose	1238:1241	arg1	cells					1215:1219	mammalian cells	1205:1219	mammalian cells at the effective dose	1205:1241	Furthermore, the optimal mannose-functionalized polymer demonstrates mannose receptor-mediated intracellular bactericidal activity against BCG mycobacteria without inducing cytotoxicity on mammalian cells at the effective dose.
27028263	2	47	theme	group	522:526	arg1	units					536:540	protected mannose group (ipman) units	504:540	protected mannose group (ipman) units	504:540	The distributions and compositions of pendent benzyl chloride and protected mannose group (ipman) units are facilely modulated by varying the polymerization sequence and feed ratio of the monomers, allowing precise control over the molecular composition, and the resulting polymers have narrow molecular weight distribution.
27028263	4	48	theme	intracellular	1111:1123	arg1	activity					1138:1145	mannose receptor-mediated intracellular bactericidal activity	1085:1145	mannose receptor-mediated intracellular bactericidal activity against BCG mycobacteria	1085:1170	Furthermore, the optimal mannose-functionalized polymer demonstrates mannose receptor-mediated intracellular bactericidal activity against BCG mycobacteria without inducing cytotoxicity on mammalian cells at the effective dose.
27028263	5	49	contain	have	1297:1300	arg2	use					1312:1314	potential use	1302:1314	potential use as antimicrobial agents against diseases such as tuberculosis, which is caused by intracellular bacteria	1302:1419	Taken together, the materials designed in this study have potential use as antimicrobial agents against diseases such as tuberculosis, which is caused by intracellular bacteria.
27028263	5	49	contain	have	1297:1300	arg1	materials					1264:1272	the materials	1260:1272	the materials designed in this study	1260:1295	Taken together, the materials designed in this study have potential use as antimicrobial agents against diseases such as tuberculosis, which is caused by intracellular bacteria.
27028263	2	50	dep	distributions	442:454	arg1	The					438:440	The	438:440	The	438:440	The distributions and compositions of pendent benzyl chloride and protected mannose group (ipman) units are facilely modulated by varying the polymerization sequence and feed ratio of the monomers, allowing precise control over the molecular composition, and the resulting polymers have narrow molecular weight distribution.
27028263	2	51	dep	sequence	595:602	arg1	the					576:578	the	576:578	the	576:578	The distributions and compositions of pendent benzyl chloride and protected mannose group (ipman) units are facilely modulated by varying the polymerization sequence and feed ratio of the monomers, allowing precise control over the molecular composition, and the resulting polymers have narrow molecular weight distribution.
27028263	2	52	contain	have	720:723	arg1	polymers					711:718	the resulting polymers	697:718	the resulting polymers	697:718	The distributions and compositions of pendent benzyl chloride and protected mannose group (ipman) units are facilely modulated by varying the polymerization sequence and feed ratio of the monomers, allowing precise control over the molecular composition, and the resulting polymers have narrow molecular weight distribution.
27028263	2	52	contain	have	720:723	arg2	distribution					749:760	narrow molecular weight distribution	725:760	narrow molecular weight distribution	725:760	The distributions and compositions of pendent benzyl chloride and protected mannose group (ipman) units are facilely modulated by varying the polymerization sequence and feed ratio of the monomers, allowing precise control over the molecular composition, and the resulting polymers have narrow molecular weight distribution.
27028263	2	53	theme	units	536:540	arg1	compositions					460:471	compositions	460:471	compositions	460:471	The distributions and compositions of pendent benzyl chloride and protected mannose group (ipman) units are facilely modulated by varying the polymerization sequence and feed ratio of the monomers, allowing precise control over the molecular composition, and the resulting polymers have narrow molecular weight distribution.
27028263	2	53	theme	units	536:540	arg1	distributions					442:454	distributions	442:454	distributions	442:454	The distributions and compositions of pendent benzyl chloride and protected mannose group (ipman) units are facilely modulated by varying the polymerization sequence and feed ratio of the monomers, allowing precise control over the molecular composition, and the resulting polymers have narrow molecular weight distribution.
27028263	0	54	theme	Mannose	69:75	arg1	Bacteria					91:98	Mannose for Targeting Bacteria	69:98	Mannose for Targeting Bacteria	69:98	Broad-Spectrum Antimicrobial Star Polycarbonates Functionalized with Mannose for Targeting Bacteria Residing inside Immune Cells.
27028263	3	55	theme	various	822:828	arg1	N					830:830	various N	822:830	various N	822:830	After deprotection of ipman groups and quaternization with various N,N-dimethylalkylamines, these star polymers with optimized compositions of cationic and mannose groups in block and random forms exhibit strong bactericidal activity and low hemolysis.
27028263	3	56	with	forms	954:958	arg1	compositions					890:901	optimized compositions	880:901	optimized compositions of cationic and mannose groups in block	880:941	After deprotection of ipman groups and quaternization with various N,N-dimethylalkylamines, these star polymers with optimized compositions of cationic and mannose groups in block and random forms exhibit strong bactericidal activity and low hemolysis.
27028263	4	57	theme	optimal	1033:1039	arg1	polymer					1064:1070	the optimal mannose-functionalized polymer	1029:1070	the optimal mannose-functionalized polymer	1029:1070	Furthermore, the optimal mannose-functionalized polymer demonstrates mannose receptor-mediated intracellular bactericidal activity against BCG mycobacteria without inducing cytotoxicity on mammalian cells at the effective dose.
27028263	2	58	theme	weight	742:747	arg1	distribution					749:760	narrow molecular weight distribution	725:760	narrow molecular weight distribution	725:760	The distributions and compositions of pendent benzyl chloride and protected mannose group (ipman) units are facilely modulated by varying the polymerization sequence and feed ratio of the monomers, allowing precise control over the molecular composition, and the resulting polymers have narrow molecular weight distribution.
27028263	3	59	theme	mannose	919:925	arg1	groups					927:932	cationic and mannose groups	906:932	cationic and mannose groups in block	906:941	After deprotection of ipman groups and quaternization with various N,N-dimethylalkylamines, these star polymers with optimized compositions of cationic and mannose groups in block and random forms exhibit strong bactericidal activity and low hemolysis.
27028263	4	60	theme	BCG	1155:1157	arg1	mycobacteria					1159:1170	BCG mycobacteria	1155:1170	BCG mycobacteria	1155:1170	Furthermore, the optimal mannose-functionalized polymer demonstrates mannose receptor-mediated intracellular bactericidal activity against BCG mycobacteria without inducing cytotoxicity on mammalian cells at the effective dose.
27028263	2	61	theme	molecular	732:740	arg1	distribution					749:760	narrow molecular weight distribution	725:760	narrow molecular weight distribution	725:760	The distributions and compositions of pendent benzyl chloride and protected mannose group (ipman) units are facilely modulated by varying the polymerization sequence and feed ratio of the monomers, allowing precise control over the molecular composition, and the resulting polymers have narrow molecular weight distribution.
27028263	3	62	theme	groups	927:932	arg1	compositions					890:901	optimized compositions	880:901	optimized compositions of cationic and mannose groups in block	880:941	After deprotection of ipman groups and quaternization with various N,N-dimethylalkylamines, these star polymers with optimized compositions of cationic and mannose groups in block and random forms exhibit strong bactericidal activity and low hemolysis.
27028263	1	63	theme	benzyl	261:266	arg1	BnCl					278:281	BnCl	278:281	BnCl	278:281	In this study, a series of star-shaped polycarbonates are synthesized by metal-free organocatalytic ring-opening polymerization of benzyl chloride (BnCl) and mannose-functionalized cyclic carbonate monomers (MTC-BnCl and MTC-ipman) with heptakis-(2,3-di-O-acetyl)-β-cyclodextrin (DA-β-CD) as macroinitiator.
27028263	1	63	theme	benzyl	261:266	arg1	chloride					268:275	benzyl chloride	261:275	benzyl chloride (BnCl)	261:282	In this study, a series of star-shaped polycarbonates are synthesized by metal-free organocatalytic ring-opening polymerization of benzyl chloride (BnCl) and mannose-functionalized cyclic carbonate monomers (MTC-BnCl and MTC-ipman) with heptakis-(2,3-di-O-acetyl)-β-cyclodextrin (DA-β-CD) as macroinitiator.
27028263	2	64	theme	feed	608:611	arg1	ratio					613:617	feed ratio	608:617	feed ratio	608:617	The distributions and compositions of pendent benzyl chloride and protected mannose group (ipman) units are facilely modulated by varying the polymerization sequence and feed ratio of the monomers, allowing precise control over the molecular composition, and the resulting polymers have narrow molecular weight distribution.
27028263	3	65	theme	cationic	906:913	arg1	groups					927:932	cationic and mannose groups	906:932	cationic and mannose groups in block	906:941	After deprotection of ipman groups and quaternization with various N,N-dimethylalkylamines, these star polymers with optimized compositions of cationic and mannose groups in block and random forms exhibit strong bactericidal activity and low hemolysis.
27028263	1	66	theme	chloride	268:275	arg1	MTC-ipman					351:359	MTC-ipman	351:359	MTC-ipman	351:359	In this study, a series of star-shaped polycarbonates are synthesized by metal-free organocatalytic ring-opening polymerization of benzyl chloride (BnCl) and mannose-functionalized cyclic carbonate monomers (MTC-BnCl and MTC-ipman) with heptakis-(2,3-di-O-acetyl)-β-cyclodextrin (DA-β-CD) as macroinitiator.
27028263	1	66	theme	chloride	268:275	arg1	MTC-BnCl					338:345	MTC-BnCl	338:345	MTC-BnCl	338:345	In this study, a series of star-shaped polycarbonates are synthesized by metal-free organocatalytic ring-opening polymerization of benzyl chloride (BnCl) and mannose-functionalized cyclic carbonate monomers (MTC-BnCl and MTC-ipman) with heptakis-(2,3-di-O-acetyl)-β-cyclodextrin (DA-β-CD) as macroinitiator.
27028263	1	66	theme	chloride	268:275	arg1	monomers					328:335	benzyl chloride (BnCl) and mannose-functionalized cyclic carbonate monomers	261:335	benzyl chloride (BnCl) and mannose-functionalized cyclic carbonate monomers (MTC-BnCl and MTC-ipman)	261:360	In this study, a series of star-shaped polycarbonates are synthesized by metal-free organocatalytic ring-opening polymerization of benzyl chloride (BnCl) and mannose-functionalized cyclic carbonate monomers (MTC-BnCl and MTC-ipman) with heptakis-(2,3-di-O-acetyl)-β-cyclodextrin (DA-β-CD) as macroinitiator.
27028263	4	67	theme	mannose-functionalized	1041:1062	arg1	polymer					1064:1070	the optimal mannose-functionalized polymer	1029:1070	the optimal mannose-functionalized polymer	1029:1070	Furthermore, the optimal mannose-functionalized polymer demonstrates mannose receptor-mediated intracellular bactericidal activity against BCG mycobacteria without inducing cytotoxicity on mammalian cells at the effective dose.
27028263	3	68	from	compositions	890:901	arg1	block					937:941	block	937:941	block	937:941	After deprotection of ipman groups and quaternization with various N,N-dimethylalkylamines, these star polymers with optimized compositions of cationic and mannose groups in block and random forms exhibit strong bactericidal activity and low hemolysis.
27028263	3	69	with	deprotection	769:780	arg1	N					830:830	various N	822:830	various N	822:830	After deprotection of ipman groups and quaternization with various N,N-dimethylalkylamines, these star polymers with optimized compositions of cationic and mannose groups in block and random forms exhibit strong bactericidal activity and low hemolysis.
27028263	2	70	theme	mannose	514:520	arg1	units					536:540	protected mannose group (ipman) units	504:540	protected mannose group (ipman) units	504:540	The distributions and compositions of pendent benzyl chloride and protected mannose group (ipman) units are facilely modulated by varying the polymerization sequence and feed ratio of the monomers, allowing precise control over the molecular composition, and the resulting polymers have narrow molecular weight distribution.
27028263	1	71	theme	heptakis-	367:375	arg1	DA-β-CD					410:416	DA-β-CD	410:416	DA-β-CD	410:416	In this study, a series of star-shaped polycarbonates are synthesized by metal-free organocatalytic ring-opening polymerization of benzyl chloride (BnCl) and mannose-functionalized cyclic carbonate monomers (MTC-BnCl and MTC-ipman) with heptakis-(2,3-di-O-acetyl)-β-cyclodextrin (DA-β-CD) as macroinitiator.
27028263	1	71	theme	heptakis-	367:375	arg1	-β-cyclodextrin					393:407	heptakis-(2,3-di-O-acetyl)-β-cyclodextrin	367:407	heptakis-(2,3-di-O-acetyl)-β-cyclodextrin (DA-β-CD)	367:417	In this study, a series of star-shaped polycarbonates are synthesized by metal-free organocatalytic ring-opening polymerization of benzyl chloride (BnCl) and mannose-functionalized cyclic carbonate monomers (MTC-BnCl and MTC-ipman) with heptakis-(2,3-di-O-acetyl)-β-cyclodextrin (DA-β-CD) as macroinitiator.
27028263	2	72	theme	pendent	476:482	arg1	chloride					491:498	pendent benzyl chloride	476:498	pendent benzyl chloride	476:498	The distributions and compositions of pendent benzyl chloride and protected mannose group (ipman) units are facilely modulated by varying the polymerization sequence and feed ratio of the monomers, allowing precise control over the molecular composition, and the resulting polymers have narrow molecular weight distribution.
27028263	3	73	theme	star	861:864	arg1	polymers					866:873	these star polymers	855:873	these star polymers with optimized compositions of cationic and mannose groups in block	855:941	After deprotection of ipman groups and quaternization with various N,N-dimethylalkylamines, these star polymers with optimized compositions of cationic and mannose groups in block and random forms exhibit strong bactericidal activity and low hemolysis.
28523632	5	0	theme	various	993:999	arg1	properties					1001:1010	various properties	993:1010	various properties of composite excipients	993:1034	The results showed that the impacts of the two factors on various properties of composite excipients showed great similarity, despite of significantly different primary properties of the parent fillers, and the HPMC level was the main contributor to the majority of the impacts.
28523632	4	1	from	effects	768:774	arg1	properties					861:870	various powder, tableting, and tablet properties	823:870	various powder, tableting, and tablet properties of composite excipients	823:894	The five-level central composite design-response surface methodology was used (i) to investigate the effects of HPMC level and solid content of the feed on various powder, tableting, and tablet properties of composite excipients, and (ii) to optimize the composition.
28523632	3	2	dep	fillers	532:538	arg1	macromolecules					554:567	organic macromolecules	546:567	organic macromolecules	546:567	Starch, calcium hydrogen phosphate dihydrate (DCPD), and mannitol were chosen as a representative of three types of commonly used fillers (i.e. organic macromolecules, water-insoluble inorganic salts, and water-soluble small molecular carbohydrates), respectively.
28523632	3	2	dep	fillers	532:538	arg1	salts					596:600	water-insoluble inorganic salts	570:600	water-insoluble inorganic salts	570:600	Starch, calcium hydrogen phosphate dihydrate (DCPD), and mannitol were chosen as a representative of three types of commonly used fillers (i.e. organic macromolecules, water-insoluble inorganic salts, and water-soluble small molecular carbohydrates), respectively.
28523632	3	2	dep	fillers	532:538	arg1	carbohydrates					637:649	water-soluble small molecular carbohydrates	607:649	water-soluble small molecular carbohydrates	607:649	Starch, calcium hydrogen phosphate dihydrate (DCPD), and mannitol were chosen as a representative of three types of commonly used fillers (i.e. organic macromolecules, water-insoluble inorganic salts, and water-soluble small molecular carbohydrates), respectively.
28523632	5	3	theme	factors	982:988	arg1	impacts					963:969	the impacts	959:969	the impacts of the two factors on various properties of composite excipients	959:1034	The results showed that the impacts of the two factors on various properties of composite excipients showed great similarity, despite of significantly different primary properties of the parent fillers, and the HPMC level was the main contributor to the majority of the impacts.
28523632	1	4	theme	flowability	230:240	arg1	properties					208:217	insufficient properties	195:217	insufficient properties	195:217	Many commonly used tablet fillers are not suitable for direct compaction process due to insufficient properties, mainly of flowability and compactability.
28523632	0	5	theme	Compaction	58:67	arg1	Properties					69:78	Direct Compaction Properties	51:78	Direct Compaction Properties of Various Tablet Fillers	51:104	Co-spray Drying with HPMC as a Platform to Improve Direct Compaction Properties of Various Tablet Fillers.
28523632	4	6	theme	powder	831:836	arg1	properties					861:870	various powder, tableting, and tablet properties	823:870	various powder, tableting, and tablet properties of composite excipients	823:894	The five-level central composite design-response surface methodology was used (i) to investigate the effects of HPMC level and solid content of the feed on various powder, tableting, and tablet properties of composite excipients, and (ii) to optimize the composition.
28523632	6	7	theme	various	1291:1297	arg1	parameters					1309:1318	various tableting parameters	1291:1318	various tableting parameters	1291:1318	An increase in HPMC level significantly improved tablet tensile strength and various tableting parameters.
28523632	4	8	theme	five-level	671:680	arg1	methodology					724:734	The five-level central composite design-response surface methodology	667:734	The five-level central composite design-response surface methodology	667:734	The five-level central composite design-response surface methodology was used (i) to investigate the effects of HPMC level and solid content of the feed on various powder, tableting, and tablet properties of composite excipients, and (ii) to optimize the composition.
28523632	5	9	theme	HPMC	1146:1149	arg1	level					1151:1155	the HPMC level	1142:1155	the HPMC level	1142:1155	The results showed that the impacts of the two factors on various properties of composite excipients showed great similarity, despite of significantly different primary properties of the parent fillers, and the HPMC level was the main contributor to the majority of the impacts.
28523632	5	9	theme	HPMC	1146:1149	arg1	contributor					1170:1180	the main contributor	1161:1180	the main contributor to the majority of the impacts	1161:1211	The results showed that the impacts of the two factors on various properties of composite excipients showed great similarity, despite of significantly different primary properties of the parent fillers, and the HPMC level was the main contributor to the majority of the impacts.
28523632	0	10	theme	Various	83:89	arg1	Fillers					98:104	Various Tablet Fillers	83:104	Various Tablet Fillers	83:104	Co-spray Drying with HPMC as a Platform to Improve Direct Compaction Properties of Various Tablet Fillers.
28523632	5	11	theme	different	1086:1094	arg1	properties					1104:1113	significantly different primary properties	1072:1113	significantly different primary properties of the parent fillers	1072:1135	The results showed that the impacts of the two factors on various properties of composite excipients showed great similarity, despite of significantly different primary properties of the parent fillers, and the HPMC level was the main contributor to the majority of the impacts.
28523632	5	12	theme	fillers	1129:1135	arg1	properties					1104:1113	significantly different primary properties	1072:1113	significantly different primary properties of the parent fillers	1072:1135	The results showed that the impacts of the two factors on various properties of composite excipients showed great similarity, despite of significantly different primary properties of the parent fillers, and the HPMC level was the main contributor to the majority of the impacts.
28523632	3	13	theme	types	509:513	arg1	mannitol					459:466	mannitol	459:466	mannitol	459:466	Starch, calcium hydrogen phosphate dihydrate (DCPD), and mannitol were chosen as a representative of three types of commonly used fillers (i.e. organic macromolecules, water-insoluble inorganic salts, and water-soluble small molecular carbohydrates), respectively.
28523632	3	13	theme	types	509:513	arg1	Starch					402:407	Starch	402:407	Starch	402:407	Starch, calcium hydrogen phosphate dihydrate (DCPD), and mannitol were chosen as a representative of three types of commonly used fillers (i.e. organic macromolecules, water-insoluble inorganic salts, and water-soluble small molecular carbohydrates), respectively.
28523632	3	13	theme	types	509:513	arg1	dihydrate					437:445	calcium hydrogen phosphate dihydrate	410:445	calcium hydrogen phosphate dihydrate (DCPD)	410:452	Starch, calcium hydrogen phosphate dihydrate (DCPD), and mannitol were chosen as a representative of three types of commonly used fillers (i.e. organic macromolecules, water-insoluble inorganic salts, and water-soluble small molecular carbohydrates), respectively.
28523632	3	13	theme	types	509:513	arg1	representative					485:498	a representative	483:498	a representative of three types of commonly used fillers (i.e. organic macromolecules, water-insoluble inorganic salts, and water-soluble small molecular carbohydrates)	483:650	Starch, calcium hydrogen phosphate dihydrate (DCPD), and mannitol were chosen as a representative of three types of commonly used fillers (i.e. organic macromolecules, water-insoluble inorganic salts, and water-soluble small molecular carbohydrates), respectively.
28523632	4	14	used	used	740:743	arg2	methodology					724:734	The five-level central composite design-response surface methodology	667:734	The five-level central composite design-response surface methodology	667:734	The five-level central composite design-response surface methodology was used (i) to investigate the effects of HPMC level and solid content of the feed on various powder, tableting, and tablet properties of composite excipients, and (ii) to optimize the composition.
28523632	1	15	theme	compactability	246:259	arg1	properties					208:217	insufficient properties	195:217	insufficient properties	195:217	Many commonly used tablet fillers are not suitable for direct compaction process due to insufficient properties, mainly of flowability and compactability.
28523632	6	16	theme	tableting	1299:1307	arg1	parameters					1309:1318	various tableting parameters	1291:1318	various tableting parameters	1291:1318	An increase in HPMC level significantly improved tablet tensile strength and various tableting parameters.
28523632	7	17	theme	established	1401:1411	arg1	models					1413:1418	the established models	1397:1418	the established models	1397:1418	For all the three fillers, their optimized composite excipients provided by the established models showed excellent performances as predicted.
28523632	4	18	theme	tableting	839:847	arg1	properties					861:870	various powder, tableting, and tablet properties	823:870	various powder, tableting, and tablet properties of composite excipients	823:894	The five-level central composite design-response surface methodology was used (i) to investigate the effects of HPMC level and solid content of the feed on various powder, tableting, and tablet properties of composite excipients, and (ii) to optimize the composition.
28523632	4	19	theme	central	682:688	arg1	methodology					724:734	The five-level central composite design-response surface methodology	667:734	The five-level central composite design-response surface methodology	667:734	The five-level central composite design-response surface methodology was used (i) to investigate the effects of HPMC level and solid content of the feed on various powder, tableting, and tablet properties of composite excipients, and (ii) to optimize the composition.
28523632	5	20	from	impacts	963:969	arg1	properties					1001:1010	various properties	993:1010	various properties of composite excipients	993:1034	The results showed that the impacts of the two factors on various properties of composite excipients showed great similarity, despite of significantly different primary properties of the parent fillers, and the HPMC level was the main contributor to the majority of the impacts.
28523632	4	21	theme	surface	716:722	arg1	methodology					724:734	The five-level central composite design-response surface methodology	667:734	The five-level central composite design-response surface methodology	667:734	The five-level central composite design-response surface methodology was used (i) to investigate the effects of HPMC level and solid content of the feed on various powder, tableting, and tablet properties of composite excipients, and (ii) to optimize the composition.
28523632	0	22	theme	Fillers	98:104	arg1	Properties					69:78	Direct Compaction Properties	51:78	Direct Compaction Properties of Various Tablet Fillers	51:104	Co-spray Drying with HPMC as a Platform to Improve Direct Compaction Properties of Various Tablet Fillers.
28523632	5	23	theme	great	1043:1047	arg1	similarity					1049:1058	great similarity	1043:1058	great similarity	1043:1058	The results showed that the impacts of the two factors on various properties of composite excipients showed great similarity, despite of significantly different primary properties of the parent fillers, and the HPMC level was the main contributor to the majority of the impacts.
28523632	4	24	theme	feed	815:818	arg1	level					784:788	HPMC level	779:788	HPMC level	779:788	The five-level central composite design-response surface methodology was used (i) to investigate the effects of HPMC level and solid content of the feed on various powder, tableting, and tablet properties of composite excipients, and (ii) to optimize the composition.
28523632	4	24	theme	feed	815:818	arg1	content					800:806	solid content	794:806	solid content of the feed	794:818	The five-level central composite design-response surface methodology was used (i) to investigate the effects of HPMC level and solid content of the feed on various powder, tableting, and tablet properties of composite excipients, and (ii) to optimize the composition.
28523632	7	25	theme	optimized	1354:1362	arg1	excipients					1374:1383	their optimized composite excipients	1348:1383	their optimized composite excipients provided by the established models	1348:1418	For all the three fillers, their optimized composite excipients provided by the established models showed excellent performances as predicted.
28523632	3	26	dep	macromolecules	554:567	arg1	i.e.					541:544	i.e.	541:544	i.e.	541:544	Starch, calcium hydrogen phosphate dihydrate (DCPD), and mannitol were chosen as a representative of three types of commonly used fillers (i.e. organic macromolecules, water-insoluble inorganic salts, and water-soluble small molecular carbohydrates), respectively.
28523632	2	27	theme	various	378:384	arg1	fillers					393:399	various tablet fillers	378:399	various tablet fillers	378:399	This work therefore aimed to use co-spray drying with HPMC as a platform to improve direct compaction properties of various tablet fillers.
28523632	0	28	theme	Tablet	91:96	arg1	Fillers					98:104	Various Tablet Fillers	83:104	Various Tablet Fillers	83:104	Co-spray Drying with HPMC as a Platform to Improve Direct Compaction Properties of Various Tablet Fillers.
28523632	3	29	theme	calcium	410:416	arg1	mannitol					459:466	mannitol	459:466	mannitol	459:466	Starch, calcium hydrogen phosphate dihydrate (DCPD), and mannitol were chosen as a representative of three types of commonly used fillers (i.e. organic macromolecules, water-insoluble inorganic salts, and water-soluble small molecular carbohydrates), respectively.
28523632	3	29	theme	calcium	410:416	arg1	Starch					402:407	Starch	402:407	Starch	402:407	Starch, calcium hydrogen phosphate dihydrate (DCPD), and mannitol were chosen as a representative of three types of commonly used fillers (i.e. organic macromolecules, water-insoluble inorganic salts, and water-soluble small molecular carbohydrates), respectively.
28523632	3	29	theme	calcium	410:416	arg1	DCPD					448:451	DCPD	448:451	DCPD	448:451	Starch, calcium hydrogen phosphate dihydrate (DCPD), and mannitol were chosen as a representative of three types of commonly used fillers (i.e. organic macromolecules, water-insoluble inorganic salts, and water-soluble small molecular carbohydrates), respectively.
28523632	3	29	theme	calcium	410:416	arg1	dihydrate					437:445	calcium hydrogen phosphate dihydrate	410:445	calcium hydrogen phosphate dihydrate (DCPD)	410:452	Starch, calcium hydrogen phosphate dihydrate (DCPD), and mannitol were chosen as a representative of three types of commonly used fillers (i.e. organic macromolecules, water-insoluble inorganic salts, and water-soluble small molecular carbohydrates), respectively.
28523632	3	29	theme	calcium	410:416	arg1	representative					485:498	a representative	483:498	a representative of three types of commonly used fillers (i.e. organic macromolecules, water-insoluble inorganic salts, and water-soluble small molecular carbohydrates)	483:650	Starch, calcium hydrogen phosphate dihydrate (DCPD), and mannitol were chosen as a representative of three types of commonly used fillers (i.e. organic macromolecules, water-insoluble inorganic salts, and water-soluble small molecular carbohydrates), respectively.
28523632	7	30	theme	composite	1364:1372	arg1	excipients					1374:1383	their optimized composite excipients	1348:1383	their optimized composite excipients provided by the established models	1348:1418	For all the three fillers, their optimized composite excipients provided by the established models showed excellent performances as predicted.
28523632	3	31	theme	phosphate	427:435	arg1	mannitol					459:466	mannitol	459:466	mannitol	459:466	Starch, calcium hydrogen phosphate dihydrate (DCPD), and mannitol were chosen as a representative of three types of commonly used fillers (i.e. organic macromolecules, water-insoluble inorganic salts, and water-soluble small molecular carbohydrates), respectively.
28523632	3	31	theme	phosphate	427:435	arg1	Starch					402:407	Starch	402:407	Starch	402:407	Starch, calcium hydrogen phosphate dihydrate (DCPD), and mannitol were chosen as a representative of three types of commonly used fillers (i.e. organic macromolecules, water-insoluble inorganic salts, and water-soluble small molecular carbohydrates), respectively.
28523632	3	31	theme	phosphate	427:435	arg1	DCPD					448:451	DCPD	448:451	DCPD	448:451	Starch, calcium hydrogen phosphate dihydrate (DCPD), and mannitol were chosen as a representative of three types of commonly used fillers (i.e. organic macromolecules, water-insoluble inorganic salts, and water-soluble small molecular carbohydrates), respectively.
28523632	3	31	theme	phosphate	427:435	arg1	dihydrate					437:445	calcium hydrogen phosphate dihydrate	410:445	calcium hydrogen phosphate dihydrate (DCPD)	410:452	Starch, calcium hydrogen phosphate dihydrate (DCPD), and mannitol were chosen as a representative of three types of commonly used fillers (i.e. organic macromolecules, water-insoluble inorganic salts, and water-soluble small molecular carbohydrates), respectively.
28523632	3	31	theme	phosphate	427:435	arg1	representative					485:498	a representative	483:498	a representative of three types of commonly used fillers (i.e. organic macromolecules, water-insoluble inorganic salts, and water-soluble small molecular carbohydrates)	483:650	Starch, calcium hydrogen phosphate dihydrate (DCPD), and mannitol were chosen as a representative of three types of commonly used fillers (i.e. organic macromolecules, water-insoluble inorganic salts, and water-soluble small molecular carbohydrates), respectively.
28523632	4	32	theme	various	823:829	arg1	properties					861:870	various powder, tableting, and tablet properties	823:870	various powder, tableting, and tablet properties of composite excipients	823:894	The five-level central composite design-response surface methodology was used (i) to investigate the effects of HPMC level and solid content of the feed on various powder, tableting, and tablet properties of composite excipients, and (ii) to optimize the composition.
28523632	3	33	theme	fillers	532:538	arg1	types					509:513	three types	503:513	three types of commonly used fillers (i.e. organic macromolecules, water-insoluble inorganic salts, and water-soluble small molecular carbohydrates)	503:650	Starch, calcium hydrogen phosphate dihydrate (DCPD), and mannitol were chosen as a representative of three types of commonly used fillers (i.e. organic macromolecules, water-insoluble inorganic salts, and water-soluble small molecular carbohydrates), respectively.
28523632	1	34	theme	direct	162:167	arg1	process					180:186	direct compaction process	162:186	direct compaction process due to insufficient properties, mainly of flowability and compactability	162:259	Many commonly used tablet fillers are not suitable for direct compaction process due to insufficient properties, mainly of flowability and compactability.
28523632	0	35	theme	Co-spray	0:7	arg1	Drying					9:14	Co-spray Drying	0:14	Co-spray Drying with HPMC as a Platform to Improve Direct Compaction Properties of Various Tablet Fillers.	0:105	Co-spray Drying with HPMC as a Platform to Improve Direct Compaction Properties of Various Tablet Fillers.
28523632	5	36	theme	parent	1122:1127	arg1	fillers					1129:1135	the parent fillers	1118:1135	the parent fillers	1118:1135	The results showed that the impacts of the two factors on various properties of composite excipients showed great similarity, despite of significantly different primary properties of the parent fillers, and the HPMC level was the main contributor to the majority of the impacts.
28523632	5	37	theme	excipients	1025:1034	arg1	properties					1001:1010	various properties	993:1010	various properties of composite excipients	993:1034	The results showed that the impacts of the two factors on various properties of composite excipients showed great similarity, despite of significantly different primary properties of the parent fillers, and the HPMC level was the main contributor to the majority of the impacts.
28523632	2	38	theme	co-spray	295:302	arg1	platform					326:333	a platform	324:333	a platform to improve direct compaction properties of various tablet fillers	324:399	This work therefore aimed to use co-spray drying with HPMC as a platform to improve direct compaction properties of various tablet fillers.
28523632	2	38	theme	co-spray	295:302	arg1	drying					304:309	co-spray drying	295:309	co-spray drying with HPMC	295:319	This work therefore aimed to use co-spray drying with HPMC as a platform to improve direct compaction properties of various tablet fillers.
28523632	6	39	theme	tensile	1270:1276	arg1	strength					1278:1285	tablet tensile strength	1263:1285	tablet tensile strength	1263:1285	An increase in HPMC level significantly improved tablet tensile strength and various tableting parameters.
28523632	2	40	theme	compaction	353:362	arg1	properties					364:373	direct compaction properties	346:373	direct compaction properties of various tablet fillers	346:399	This work therefore aimed to use co-spray drying with HPMC as a platform to improve direct compaction properties of various tablet fillers.
28523632	4	41	theme	level	784:788	arg1	effects					768:774	the effects	764:774	the effects of HPMC level and solid content of the feed on various powder, tableting, and tablet properties of composite excipients	764:894	The five-level central composite design-response surface methodology was used (i) to investigate the effects of HPMC level and solid content of the feed on various powder, tableting, and tablet properties of composite excipients, and (ii) to optimize the composition.
28523632	4	42	theme	excipients	885:894	arg1	properties					861:870	various powder, tableting, and tablet properties	823:870	various powder, tableting, and tablet properties of composite excipients	823:894	The five-level central composite design-response surface methodology was used (i) to investigate the effects of HPMC level and solid content of the feed on various powder, tableting, and tablet properties of composite excipients, and (ii) to optimize the composition.
28523632	4	43	theme	content	800:806	arg1	effects					768:774	the effects	764:774	the effects of HPMC level and solid content of the feed on various powder, tableting, and tablet properties of composite excipients	764:894	The five-level central composite design-response surface methodology was used (i) to investigate the effects of HPMC level and solid content of the feed on various powder, tableting, and tablet properties of composite excipients, and (ii) to optimize the composition.
28523632	4	44	theme	composite	875:883	arg1	excipients					885:894	composite excipients	875:894	composite excipients	875:894	The five-level central composite design-response surface methodology was used (i) to investigate the effects of HPMC level and solid content of the feed on various powder, tableting, and tablet properties of composite excipients, and (ii) to optimize the composition.
28523632	3	45	theme	small	621:625	arg1	carbohydrates					637:649	water-soluble small molecular carbohydrates	607:649	water-soluble small molecular carbohydrates	607:649	Starch, calcium hydrogen phosphate dihydrate (DCPD), and mannitol were chosen as a representative of three types of commonly used fillers (i.e. organic macromolecules, water-insoluble inorganic salts, and water-soluble small molecular carbohydrates), respectively.
28523632	3	46	theme	used	527:530	arg1	fillers					532:538	commonly used fillers	518:538	commonly used fillers (i.e. organic macromolecules, water-insoluble inorganic salts, and water-soluble small molecular carbohydrates)	518:650	Starch, calcium hydrogen phosphate dihydrate (DCPD), and mannitol were chosen as a representative of three types of commonly used fillers (i.e. organic macromolecules, water-insoluble inorganic salts, and water-soluble small molecular carbohydrates), respectively.
28523632	1	47	theme	due	188:190	arg1	process					180:186	direct compaction process	162:186	direct compaction process due to insufficient properties, mainly of flowability and compactability	162:259	Many commonly used tablet fillers are not suitable for direct compaction process due to insufficient properties, mainly of flowability and compactability.
28523632	4	48	theme	composite	690:698	arg1	methodology					724:734	The five-level central composite design-response surface methodology	667:734	The five-level central composite design-response surface methodology	667:734	The five-level central composite design-response surface methodology was used (i) to investigate the effects of HPMC level and solid content of the feed on various powder, tableting, and tablet properties of composite excipients, and (ii) to optimize the composition.
28523632	6	49	theme	tablet	1263:1268	arg1	strength					1278:1285	tablet tensile strength	1263:1285	tablet tensile strength	1263:1285	An increase in HPMC level significantly improved tablet tensile strength and various tableting parameters.
28523632	3	50	theme	molecular	627:635	arg1	carbohydrates					637:649	water-soluble small molecular carbohydrates	607:649	water-soluble small molecular carbohydrates	607:649	Starch, calcium hydrogen phosphate dihydrate (DCPD), and mannitol were chosen as a representative of three types of commonly used fillers (i.e. organic macromolecules, water-insoluble inorganic salts, and water-soluble small molecular carbohydrates), respectively.
28523632	4	51	theme	solid	794:798	arg1	content					800:806	solid content	794:806	solid content of the feed	794:818	The five-level central composite design-response surface methodology was used (i) to investigate the effects of HPMC level and solid content of the feed on various powder, tableting, and tablet properties of composite excipients, and (ii) to optimize the composition.
28523632	1	52	theme	compaction	169:178	arg1	process					180:186	direct compaction process	162:186	direct compaction process due to insufficient properties, mainly of flowability and compactability	162:259	Many commonly used tablet fillers are not suitable for direct compaction process due to insufficient properties, mainly of flowability and compactability.
28523632	2	53	theme	fillers	393:399	arg1	properties					364:373	direct compaction properties	346:373	direct compaction properties of various tablet fillers	346:399	This work therefore aimed to use co-spray drying with HPMC as a platform to improve direct compaction properties of various tablet fillers.
28523632	3	54	theme	hydrogen	418:425	arg1	mannitol					459:466	mannitol	459:466	mannitol	459:466	Starch, calcium hydrogen phosphate dihydrate (DCPD), and mannitol were chosen as a representative of three types of commonly used fillers (i.e. organic macromolecules, water-insoluble inorganic salts, and water-soluble small molecular carbohydrates), respectively.
28523632	3	54	theme	hydrogen	418:425	arg1	Starch					402:407	Starch	402:407	Starch	402:407	Starch, calcium hydrogen phosphate dihydrate (DCPD), and mannitol were chosen as a representative of three types of commonly used fillers (i.e. organic macromolecules, water-insoluble inorganic salts, and water-soluble small molecular carbohydrates), respectively.
28523632	3	54	theme	hydrogen	418:425	arg1	DCPD					448:451	DCPD	448:451	DCPD	448:451	Starch, calcium hydrogen phosphate dihydrate (DCPD), and mannitol were chosen as a representative of three types of commonly used fillers (i.e. organic macromolecules, water-insoluble inorganic salts, and water-soluble small molecular carbohydrates), respectively.
28523632	3	54	theme	hydrogen	418:425	arg1	dihydrate					437:445	calcium hydrogen phosphate dihydrate	410:445	calcium hydrogen phosphate dihydrate (DCPD)	410:452	Starch, calcium hydrogen phosphate dihydrate (DCPD), and mannitol were chosen as a representative of three types of commonly used fillers (i.e. organic macromolecules, water-insoluble inorganic salts, and water-soluble small molecular carbohydrates), respectively.
28523632	3	54	theme	hydrogen	418:425	arg1	representative					485:498	a representative	483:498	a representative of three types of commonly used fillers (i.e. organic macromolecules, water-insoluble inorganic salts, and water-soluble small molecular carbohydrates)	483:650	Starch, calcium hydrogen phosphate dihydrate (DCPD), and mannitol were chosen as a representative of three types of commonly used fillers (i.e. organic macromolecules, water-insoluble inorganic salts, and water-soluble small molecular carbohydrates), respectively.
28523632	5	55	theme	primary	1096:1102	arg1	properties					1104:1113	significantly different primary properties	1072:1113	significantly different primary properties of the parent fillers	1072:1135	The results showed that the impacts of the two factors on various properties of composite excipients showed great similarity, despite of significantly different primary properties of the parent fillers, and the HPMC level was the main contributor to the majority of the impacts.
28523632	5	56	theme	composite	1015:1023	arg1	excipients					1025:1034	composite excipients	1015:1034	composite excipients	1015:1034	The results showed that the impacts of the two factors on various properties of composite excipients showed great similarity, despite of significantly different primary properties of the parent fillers, and the HPMC level was the main contributor to the majority of the impacts.
28523632	0	57	dep	Platform	31:38	arg1	Improve					43:49	Improve	43:49	to Improve Direct Compaction Properties of Various Tablet Fillers	40:104	Co-spray Drying with HPMC as a Platform to Improve Direct Compaction Properties of Various Tablet Fillers.
28523632	1	58	theme	insufficient	195:206	arg1	properties					208:217	insufficient properties	195:217	insufficient properties	195:217	Many commonly used tablet fillers are not suitable for direct compaction process due to insufficient properties, mainly of flowability and compactability.
28523632	4	59	theme	tablet	854:859	arg1	properties					861:870	various powder, tableting, and tablet properties	823:870	various powder, tableting, and tablet properties of composite excipients	823:894	The five-level central composite design-response surface methodology was used (i) to investigate the effects of HPMC level and solid content of the feed on various powder, tableting, and tablet properties of composite excipients, and (ii) to optimize the composition.
28523632	3	60	theme	water-soluble	607:619	arg1	carbohydrates					637:649	water-soluble small molecular carbohydrates	607:649	water-soluble small molecular carbohydrates	607:649	Starch, calcium hydrogen phosphate dihydrate (DCPD), and mannitol were chosen as a representative of three types of commonly used fillers (i.e. organic macromolecules, water-insoluble inorganic salts, and water-soluble small molecular carbohydrates), respectively.
28523632	4	61	theme	design-response	700:714	arg1	methodology					724:734	The five-level central composite design-response surface methodology	667:734	The five-level central composite design-response surface methodology	667:734	The five-level central composite design-response surface methodology was used (i) to investigate the effects of HPMC level and solid content of the feed on various powder, tableting, and tablet properties of composite excipients, and (ii) to optimize the composition.
28523632	7	62	theme	excellent	1427:1435	arg1	performances					1437:1448	excellent performances	1427:1448	excellent performances	1427:1448	For all the three fillers, their optimized composite excipients provided by the established models showed excellent performances as predicted.
28523632	3	63	theme	organic	546:552	arg1	macromolecules					554:567	organic macromolecules	546:567	organic macromolecules	546:567	Starch, calcium hydrogen phosphate dihydrate (DCPD), and mannitol were chosen as a representative of three types of commonly used fillers (i.e. organic macromolecules, water-insoluble inorganic salts, and water-soluble small molecular carbohydrates), respectively.
28523632	0	64	with	Drying	9:14	arg1	HPMC					21:24	HPMC	21:24	HPMC	21:24	Co-spray Drying with HPMC as a Platform to Improve Direct Compaction Properties of Various Tablet Fillers.
28523632	1	65	theme	Many	107:110	arg1	fillers					133:139	Many commonly used tablet fillers	107:139	Many commonly used tablet fillers	107:139	Many commonly used tablet fillers are not suitable for direct compaction process due to insufficient properties, mainly of flowability and compactability.
28523632	5	66	theme	impacts	1205:1211	arg1	majority					1189:1196	the majority	1185:1196	the majority of the impacts	1185:1211	The results showed that the impacts of the two factors on various properties of composite excipients showed great similarity, despite of significantly different primary properties of the parent fillers, and the HPMC level was the main contributor to the majority of the impacts.
28523632	4	67	theme	HPMC	779:782	arg1	level					784:788	HPMC level	779:788	HPMC level	779:788	The five-level central composite design-response surface methodology was used (i) to investigate the effects of HPMC level and solid content of the feed on various powder, tableting, and tablet properties of composite excipients, and (ii) to optimize the composition.
28523632	6	68	theme	HPMC	1229:1232	arg1	level					1234:1238	HPMC level	1229:1238	HPMC level	1229:1238	An increase in HPMC level significantly improved tablet tensile strength and various tableting parameters.
28523632	1	69	theme	tablet	126:131	arg1	fillers					133:139	Many commonly used tablet fillers	107:139	Many commonly used tablet fillers	107:139	Many commonly used tablet fillers are not suitable for direct compaction process due to insufficient properties, mainly of flowability and compactability.
28523632	4	70	dep	optimize	909:916	arg1	ii					902:903	ii	902:903	ii	902:903	The five-level central composite design-response surface methodology was used (i) to investigate the effects of HPMC level and solid content of the feed on various powder, tableting, and tablet properties of composite excipients, and (ii) to optimize the composition.
28523632	1	71	theme	used	121:124	arg1	fillers					133:139	Many commonly used tablet fillers	107:139	Many commonly used tablet fillers	107:139	Many commonly used tablet fillers are not suitable for direct compaction process due to insufficient properties, mainly of flowability and compactability.
28523632	5	72	theme	main	1165:1168	arg1	level					1151:1155	the HPMC level	1142:1155	the HPMC level	1142:1155	The results showed that the impacts of the two factors on various properties of composite excipients showed great similarity, despite of significantly different primary properties of the parent fillers, and the HPMC level was the main contributor to the majority of the impacts.
28523632	5	72	theme	main	1165:1168	arg1	contributor					1170:1180	the main contributor	1161:1180	the main contributor to the majority of the impacts	1161:1211	The results showed that the impacts of the two factors on various properties of composite excipients showed great similarity, despite of significantly different primary properties of the parent fillers, and the HPMC level was the main contributor to the majority of the impacts.
28523632	0	73	theme	Direct	51:56	arg1	Properties					69:78	Direct Compaction Properties	51:78	Direct Compaction Properties of Various Tablet Fillers	51:104	Co-spray Drying with HPMC as a Platform to Improve Direct Compaction Properties of Various Tablet Fillers.
28523632	2	74	theme	tablet	386:391	arg1	fillers					393:399	various tablet fillers	378:399	various tablet fillers	378:399	This work therefore aimed to use co-spray drying with HPMC as a platform to improve direct compaction properties of various tablet fillers.
28523632	3	75	theme	water-insoluble	570:584	arg1	salts					596:600	water-insoluble inorganic salts	570:600	water-insoluble inorganic salts	570:600	Starch, calcium hydrogen phosphate dihydrate (DCPD), and mannitol were chosen as a representative of three types of commonly used fillers (i.e. organic macromolecules, water-insoluble inorganic salts, and water-soluble small molecular carbohydrates), respectively.
28523632	2	76	theme	direct	346:351	arg1	properties					364:373	direct compaction properties	346:373	direct compaction properties of various tablet fillers	346:399	This work therefore aimed to use co-spray drying with HPMC as a platform to improve direct compaction properties of various tablet fillers.
28523632	6	77	from	increase	1217:1224	arg1	level					1234:1238	HPMC level	1229:1238	HPMC level	1229:1238	An increase in HPMC level significantly improved tablet tensile strength and various tableting parameters.
28523632	2	78	with	drying	304:309	arg1	HPMC					316:319	HPMC	316:319	HPMC	316:319	This work therefore aimed to use co-spray drying with HPMC as a platform to improve direct compaction properties of various tablet fillers.
28523632	3	79	theme	inorganic	586:594	arg1	salts					596:600	water-insoluble inorganic salts	570:600	water-insoluble inorganic salts	570:600	Starch, calcium hydrogen phosphate dihydrate (DCPD), and mannitol were chosen as a representative of three types of commonly used fillers (i.e. organic macromolecules, water-insoluble inorganic salts, and water-soluble small molecular carbohydrates), respectively.
28523632	4	80	dep	investigate	752:762	arg1	i					746:746	i	746:746	i	746:746	The five-level central composite design-response surface methodology was used (i) to investigate the effects of HPMC level and solid content of the feed on various powder, tableting, and tablet properties of composite excipients, and (ii) to optimize the composition.
28092912	9	0	theme	mannose	1081:1087	arg1	ratio					1072:1076	the molar ratio	1062:1076	the molar ratio of mannose	1062:1087	With a reduced NaNO3 concentration, the molecular weight of welan gum and the molar ratio of mannose decreased, but the molar ratio of glucuronic acid increased.
28092912	9	0	theme	mannose	1081:1087	arg1	weight					1038:1043	the molecular weight	1024:1043	the molecular weight of welan gum	1024:1056	With a reduced NaNO3 concentration, the molecular weight of welan gum and the molar ratio of mannose decreased, but the molar ratio of glucuronic acid increased.
28092912	10	1	theme	different	1155:1163	arg1	sources					1174:1180	different nitrogen sources	1155:1180	different nitrogen sources	1155:1180	With different nitrogen sources, the acetyl content of welan gum differed but its structure was similar.
28092912	8	2	theme	solution	950:957	arg1	concentration					959:971	a welan solution concentration	942:971	a welan solution concentration of 6-10 g·L-1	942:985	In the media containing NaNO3 (3 g·L-1), welan viscosity was higher at 30-70°C and a welan solution concentration of 6-10 g·L-1.
28092912	9	3	theme	reduced	995:1001	arg1	concentration					1009:1021	a reduced NaNO3 concentration	993:1021	a reduced NaNO3 concentration	993:1021	With a reduced NaNO3 concentration, the molecular weight of welan gum and the molar ratio of mannose decreased, but the molar ratio of glucuronic acid increased.
28092912	10	4	theme	nitrogen	1165:1172	arg1	sources					1174:1180	different nitrogen sources	1155:1180	different nitrogen sources	1155:1180	With different nitrogen sources, the acetyl content of welan gum differed but its structure was similar.
28092912	9	5	theme	acid	1134:1137	arg1	ratio					1114:1118	the molar ratio	1104:1118	the molar ratio of glucuronic acid	1104:1137	With a reduced NaNO3 concentration, the molecular weight of welan gum and the molar ratio of mannose decreased, but the molar ratio of glucuronic acid increased.
28092912	4	6	theme	these	365:369	arg1	2					360:360	2	360:360	2	360:360	Six different nitrogen sources were used for ATCC 31555 fermentation, and 2 of these were further analyzed due to their more positive influence on welan gum production and bacterial biomass.
28092912	4	6	theme	these	365:369	arg1	these					365:369	these	365:369	these	365:369	Six different nitrogen sources were used for ATCC 31555 fermentation, and 2 of these were further analyzed due to their more positive influence on welan gum production and bacterial biomass.
28092912	7	7	theme	higher	831:836	arg1	concentration					844:856	a higher welan concentration	829:856	a higher welan concentration	829:856	Welan viscosity decreased at higher temperatures of 30-90°C, and it increased with a higher welan concentration.
28092912	9	8	theme	gum	1054:1056	arg1	ratio					1072:1076	the molar ratio	1062:1076	the molar ratio of mannose	1062:1087	With a reduced NaNO3 concentration, the molecular weight of welan gum and the molar ratio of mannose decreased, but the molar ratio of glucuronic acid increased.
28092912	9	8	theme	gum	1054:1056	arg1	weight					1038:1043	the molecular weight	1024:1043	the molecular weight of welan gum	1024:1056	With a reduced NaNO3 concentration, the molecular weight of welan gum and the molar ratio of mannose decreased, but the molar ratio of glucuronic acid increased.
28092912	5	9	theme	acyl	576:579	arg1	content					581:587	acyl content	576:587	acyl content	576:587	Bacterial biomass, welan gum yield, welan viscosity, molecular weight, monosaccharide composition, acyl content, and welan structure were analyzed.
28092912	9	10	theme	glucuronic	1123:1132	arg1	acid					1134:1137	glucuronic acid	1123:1137	glucuronic acid	1123:1137	With a reduced NaNO3 concentration, the molecular weight of welan gum and the molar ratio of mannose decreased, but the molar ratio of glucuronic acid increased.
28092912	6	11	theme	ATCC	679:682	arg1	higher					695:700	higher	695:700	higher	695:700	Welan gum production and the biomass concentration of ATCC 31555 were higher in media containing NaNO3 and beef extract.
28092912	6	11	theme	ATCC	679:682	arg1	concentration					662:674	the biomass concentration	650:674	the biomass concentration of ATCC 31555	650:688	Welan gum production and the biomass concentration of ATCC 31555 were higher in media containing NaNO3 and beef extract.
28092912	6	11	theme	ATCC	679:682	arg1	production					635:644	Welan gum production	625:644	Welan gum production	625:644	Welan gum production and the biomass concentration of ATCC 31555 were higher in media containing NaNO3 and beef extract.
28092912	6	12	theme	biomass	654:660	arg1	higher					695:700	higher	695:700	higher	695:700	Welan gum production and the biomass concentration of ATCC 31555 were higher in media containing NaNO3 and beef extract.
28092912	6	12	theme	biomass	654:660	arg1	concentration					662:674	the biomass concentration	650:674	the biomass concentration of ATCC 31555	650:688	Welan gum production and the biomass concentration of ATCC 31555 were higher in media containing NaNO3 and beef extract.
28092912	4	13	theme	gum	439:441	arg1	production					443:452	welan gum production	433:452	welan gum production	433:452	Six different nitrogen sources were used for ATCC 31555 fermentation, and 2 of these were further analyzed due to their more positive influence on welan gum production and bacterial biomass.
28092912	12	14	theme	NaNO3	1318:1322	arg1	concentration					1324:1336	A reduced NaNO3 concentration	1308:1336	A reduced NaNO3 concentration	1308:1336	A reduced NaNO3 concentration promoted welan viscosity.
28092912	9	15	theme	NaNO3	1003:1007	arg1	concentration					1009:1021	a reduced NaNO3 concentration	993:1021	a reduced NaNO3 concentration	993:1021	With a reduced NaNO3 concentration, the molecular weight of welan gum and the molar ratio of mannose decreased, but the molar ratio of glucuronic acid increased.
28092912	4	16	theme	bacterial	458:466	arg1	biomass					468:474	bacterial biomass	458:474	bacterial biomass	458:474	Six different nitrogen sources were used for ATCC 31555 fermentation, and 2 of these were further analyzed due to their more positive influence on welan gum production and bacterial biomass.
28092912	8	17	theme	g·L-1	981:985	arg1	concentration					959:971	a welan solution concentration	942:971	a welan solution concentration of 6-10 g·L-1	942:985	In the media containing NaNO3 (3 g·L-1), welan viscosity was higher at 30-70°C and a welan solution concentration of 6-10 g·L-1.
28092912	8	17	theme	g·L-1	981:985	arg1	30-70°C					930:936	30-70°C	930:936	30-70°C	930:936	In the media containing NaNO3 (3 g·L-1), welan viscosity was higher at 30-70°C and a welan solution concentration of 6-10 g·L-1.
28092912	5	18	theme	molecular	530:538	arg1	weight					540:545	molecular weight	530:545	molecular weight	530:545	Bacterial biomass, welan gum yield, welan viscosity, molecular weight, monosaccharide composition, acyl content, and welan structure were analyzed.
28092912	2	19	theme	Sphingomonas	257:268	arg1	sp					270:271	Sphingomonas sp	257:271	Sphingomonas sp	257:271	This study aimed to investigate the effect of nitrogen sources on the production and rheological properties of welan gum produced by Sphingomonas sp.
28092912	4	20	theme	different	290:298	arg1	sources					309:315	Six different nitrogen sources	286:315	Six different nitrogen sources	286:315	Six different nitrogen sources were used for ATCC 31555 fermentation, and 2 of these were further analyzed due to their more positive influence on welan gum production and bacterial biomass.
28092912	12	21	theme	welan	1347:1351	arg1	viscosity					1353:1361	welan viscosity	1347:1361	welan viscosity	1347:1361	A reduced NaNO3 concentration promoted welan viscosity.
28092912	5	22	theme	welan	594:598	arg1	structure					600:608	welan structure	594:608	welan structure	594:608	Bacterial biomass, welan gum yield, welan viscosity, molecular weight, monosaccharide composition, acyl content, and welan structure were analyzed.
28092912	0	23	theme	Sphingomonas	63:74	arg1	sp					76:77	Sphingomonas sp	63:77	Sphingomonas sp	63:77	Production and Rheological Properties of Welan Gum Produced by Sphingomonas sp.
28092912	5	24	theme	biomass	487:493	arg1	yield					506:510	Bacterial biomass, welan gum yield	477:510	yield	506:510	Bacterial biomass, welan gum yield, welan viscosity, molecular weight, monosaccharide composition, acyl content, and welan structure were analyzed.
28092912	6	25	contain	containing	711:720	arg2	NaNO3					722:726	NaNO3	722:726	NaNO3	722:726	Welan gum production and the biomass concentration of ATCC 31555 were higher in media containing NaNO3 and beef extract.
28092912	6	25	contain	containing	711:720	arg2	extract					737:743	beef extract	732:743	beef extract	732:743	Welan gum production and the biomass concentration of ATCC 31555 were higher in media containing NaNO3 and beef extract.
28092912	6	25	contain	containing	711:720	arg1	media					705:709	media	705:709	media containing NaNO3 and beef extract	705:743	Welan gum production and the biomass concentration of ATCC 31555 were higher in media containing NaNO3 and beef extract.
28092912	8	26	theme	welan	944:948	arg1	concentration					959:971	a welan solution concentration	942:971	a welan solution concentration of 6-10 g·L-1	942:985	In the media containing NaNO3 (3 g·L-1), welan viscosity was higher at 30-70°C and a welan solution concentration of 6-10 g·L-1.
28092912	7	27	theme	30-90°C	798:804	arg1	temperatures					782:793	higher temperatures	775:793	higher temperatures of 30-90°C	775:804	Welan viscosity decreased at higher temperatures of 30-90°C, and it increased with a higher welan concentration.
28092912	7	28	theme	higher	775:780	arg1	temperatures					782:793	higher temperatures	775:793	higher temperatures of 30-90°C	775:804	Welan viscosity decreased at higher temperatures of 30-90°C, and it increased with a higher welan concentration.
28092912	5	29	theme	monosaccharide	548:561	arg1	composition					563:573	monosaccharide composition	548:573	monosaccharide composition	548:573	Bacterial biomass, welan gum yield, welan viscosity, molecular weight, monosaccharide composition, acyl content, and welan structure were analyzed.
28092912	5	30	dep	yield	506:510	arg1	welan					496:500	Bacterial biomass, welan gum yield	477:510	welan	496:500	Bacterial biomass, welan gum yield, welan viscosity, molecular weight, monosaccharide composition, acyl content, and welan structure were analyzed.
28092912	0	31	theme	Rheological	15:25	arg1	Properties					27:36	Rheological Properties	15:36	Rheological Properties	15:36	Production and Rheological Properties of Welan Gum Produced by Sphingomonas sp.
28092912	11	32	theme	welan	1290:1294	arg1	production					1296:1305	welan production	1290:1305	welan production	1290:1305	NaNO3 and beef extract facilitated welan production.
28092912	2	33	theme	nitrogen	170:177	arg1	sources					179:185	nitrogen sources	170:185	nitrogen sources	170:185	This study aimed to investigate the effect of nitrogen sources on the production and rheological properties of welan gum produced by Sphingomonas sp.
28092912	1	34	theme	Different	96:104	arg1	Sources					115:121	Different Nitrogen Sources	96:121	Different Nitrogen Sources	96:121	ATCC 31555 with Different Nitrogen Sources.
28092912	10	35	theme	gum	1211:1213	arg1	content					1194:1200	the acetyl content	1183:1200	the acetyl content of welan gum	1183:1213	With different nitrogen sources, the acetyl content of welan gum differed but its structure was similar.
28092912	4	36	used	used	322:325	arg2	sources					309:315	Six different nitrogen sources	286:315	Six different nitrogen sources	286:315	Six different nitrogen sources were used for ATCC 31555 fermentation, and 2 of these were further analyzed due to their more positive influence on welan gum production and bacterial biomass.
28092912	0	37	theme	Welan	41:45	arg1	Gum					47:49	Welan Gum	41:49	Welan Gum	41:49	Production and Rheological Properties of Welan Gum Produced by Sphingomonas sp.
28092912	4	38	from	influence	420:428	arg1	production					443:452	welan gum production	433:452	welan gum production	433:452	Six different nitrogen sources were used for ATCC 31555 fermentation, and 2 of these were further analyzed due to their more positive influence on welan gum production and bacterial biomass.
28092912	4	38	from	influence	420:428	arg1	biomass					468:474	bacterial biomass	458:474	bacterial biomass	458:474	Six different nitrogen sources were used for ATCC 31555 fermentation, and 2 of these were further analyzed due to their more positive influence on welan gum production and bacterial biomass.
28092912	4	39	theme	positive	411:418	arg1	influence					420:428	their more positive influence	400:428	their more positive influence on welan gum production and bacterial biomass	400:474	Six different nitrogen sources were used for ATCC 31555 fermentation, and 2 of these were further analyzed due to their more positive influence on welan gum production and bacterial biomass.
28092912	6	40	theme	gum	631:633	arg1	production					635:644	Welan gum production	625:644	Welan gum production	625:644	Welan gum production and the biomass concentration of ATCC 31555 were higher in media containing NaNO3 and beef extract.
28092912	11	41	theme	beef	1265:1268	arg1	extract					1270:1276	beef extract	1265:1276	beef extract	1265:1276	NaNO3 and beef extract facilitated welan production.
28092912	4	42	theme	welan	433:437	arg1	production					443:452	welan gum production	433:452	welan gum production	433:452	Six different nitrogen sources were used for ATCC 31555 fermentation, and 2 of these were further analyzed due to their more positive influence on welan gum production and bacterial biomass.
28092912	4	43	theme	ATCC	331:334	arg1	fermentation					342:353	ATCC 31555 fermentation	331:353	ATCC 31555 fermentation	331:353	Six different nitrogen sources were used for ATCC 31555 fermentation, and 2 of these were further analyzed due to their more positive influence on welan gum production and bacterial biomass.
28092912	10	44	theme	acetyl	1187:1192	arg1	content					1194:1200	the acetyl content	1183:1200	the acetyl content of welan gum	1183:1213	With different nitrogen sources, the acetyl content of welan gum differed but its structure was similar.
28092912	7	45	theme	Welan	746:750	arg1	viscosity					752:760	Welan viscosity	746:760	Welan viscosity	746:760	Welan viscosity decreased at higher temperatures of 30-90°C, and it increased with a higher welan concentration.
28092912	10	46	theme	welan	1205:1209	arg1	gum					1211:1213	welan gum	1205:1213	welan gum	1205:1213	With different nitrogen sources, the acetyl content of welan gum differed but its structure was similar.
28092912	2	47	theme	rheological	209:219	arg1	properties					221:230	rheological properties	209:230	rheological properties	209:230	This study aimed to investigate the effect of nitrogen sources on the production and rheological properties of welan gum produced by Sphingomonas sp.
28092912	5	48	theme	welan	513:517	arg1	viscosity					519:527	welan viscosity	513:527	welan viscosity	513:527	Bacterial biomass, welan gum yield, welan viscosity, molecular weight, monosaccharide composition, acyl content, and welan structure were analyzed.
28092912	1	49	theme	Nitrogen	106:113	arg1	Sources					115:121	Different Nitrogen Sources	96:121	Different Nitrogen Sources	96:121	ATCC 31555 with Different Nitrogen Sources.
28092912	0	50	theme	Gum	47:49	arg1	Production					0:9	Production	0:9	Production	0:9	Production and Rheological Properties of Welan Gum Produced by Sphingomonas sp.
28092912	0	50	theme	Gum	47:49	arg1	Properties					27:36	Rheological Properties	15:36	Rheological Properties	15:36	Production and Rheological Properties of Welan Gum Produced by Sphingomonas sp.
28092912	8	51	contain	containing	872:881	arg2	g·L-1					892:896	3 g·L-1	890:896	3 g·L-1	890:896	In the media containing NaNO3 (3 g·L-1), welan viscosity was higher at 30-70°C and a welan solution concentration of 6-10 g·L-1.
28092912	8	51	contain	containing	872:881	arg2	NaNO3					883:887	NaNO3	883:887	NaNO3 (3 g·L-1)	883:897	In the media containing NaNO3 (3 g·L-1), welan viscosity was higher at 30-70°C and a welan solution concentration of 6-10 g·L-1.
28092912	8	51	contain	containing	872:881	arg1	media					866:870	the media	862:870	the media containing NaNO3 (3 g·L-1)	862:897	In the media containing NaNO3 (3 g·L-1), welan viscosity was higher at 30-70°C and a welan solution concentration of 6-10 g·L-1.
28092912	6	52	theme	Welan	625:629	arg1	production					635:644	Welan gum production	625:644	Welan gum production	625:644	Welan gum production and the biomass concentration of ATCC 31555 were higher in media containing NaNO3 and beef extract.
28092912	6	53	theme	beef	732:735	arg1	extract					737:743	beef extract	732:743	beef extract	732:743	Welan gum production and the biomass concentration of ATCC 31555 were higher in media containing NaNO3 and beef extract.
28092912	9	54	theme	molar	1108:1112	arg1	ratio					1114:1118	the molar ratio	1104:1118	the molar ratio of glucuronic acid	1104:1137	With a reduced NaNO3 concentration, the molecular weight of welan gum and the molar ratio of mannose decreased, but the molar ratio of glucuronic acid increased.
28092912	8	55	theme	welan	900:904	arg1	viscosity					906:914	welan viscosity	900:914	welan viscosity	900:914	In the media containing NaNO3 (3 g·L-1), welan viscosity was higher at 30-70°C and a welan solution concentration of 6-10 g·L-1.
28092912	12	56	theme	reduced	1310:1316	arg1	concentration					1324:1336	A reduced NaNO3 concentration	1308:1336	A reduced NaNO3 concentration	1308:1336	A reduced NaNO3 concentration promoted welan viscosity.
28092912	9	57	theme	welan	1048:1052	arg1	gum					1054:1056	welan gum	1048:1056	welan gum	1048:1056	With a reduced NaNO3 concentration, the molecular weight of welan gum and the molar ratio of mannose decreased, but the molar ratio of glucuronic acid increased.
28092912	9	58	theme	molar	1066:1070	arg1	ratio					1072:1076	the molar ratio	1062:1076	the molar ratio of mannose	1062:1087	With a reduced NaNO3 concentration, the molecular weight of welan gum and the molar ratio of mannose decreased, but the molar ratio of glucuronic acid increased.
28092912	9	59	theme	molecular	1028:1036	arg1	weight					1038:1043	the molecular weight	1024:1043	the molecular weight of welan gum	1024:1056	With a reduced NaNO3 concentration, the molecular weight of welan gum and the molar ratio of mannose decreased, but the molar ratio of glucuronic acid increased.
28092912	5	60	theme	Bacterial	477:485	arg1	yield					506:510	Bacterial biomass, welan gum yield	477:510	yield	506:510	Bacterial biomass, welan gum yield, welan viscosity, molecular weight, monosaccharide composition, acyl content, and welan structure were analyzed.
28092912	2	61	from	effect	160:165	arg1	properties					221:230	rheological properties	209:230	rheological properties	209:230	This study aimed to investigate the effect of nitrogen sources on the production and rheological properties of welan gum produced by Sphingomonas sp.
28092912	2	61	from	effect	160:165	arg1	production					194:203	production	194:203	production	194:203	This study aimed to investigate the effect of nitrogen sources on the production and rheological properties of welan gum produced by Sphingomonas sp.
28092912	2	62	theme	sources	179:185	arg1	effect					160:165	the effect	156:165	the effect of nitrogen sources on the production and rheological properties of welan gum produced by Sphingomonas sp	156:271	This study aimed to investigate the effect of nitrogen sources on the production and rheological properties of welan gum produced by Sphingomonas sp.
28092912	2	63	theme	gum	241:243	arg1	properties					221:230	rheological properties	209:230	rheological properties	209:230	This study aimed to investigate the effect of nitrogen sources on the production and rheological properties of welan gum produced by Sphingomonas sp.
28092912	2	63	theme	gum	241:243	arg1	production					194:203	production	194:203	production	194:203	This study aimed to investigate the effect of nitrogen sources on the production and rheological properties of welan gum produced by Sphingomonas sp.
28092912	4	64	theme	nitrogen	300:307	arg1	sources					309:315	Six different nitrogen sources	286:315	Six different nitrogen sources	286:315	Six different nitrogen sources were used for ATCC 31555 fermentation, and 2 of these were further analyzed due to their more positive influence on welan gum production and bacterial biomass.
28092912	2	65	dep	production	194:203	arg1	the					190:192	the	190:192	the	190:192	This study aimed to investigate the effect of nitrogen sources on the production and rheological properties of welan gum produced by Sphingomonas sp.
28092912	2	66	theme	welan	235:239	arg1	gum					241:243	welan gum	235:243	welan gum	235:243	This study aimed to investigate the effect of nitrogen sources on the production and rheological properties of welan gum produced by Sphingomonas sp.
28092912	5	67	theme	gum	502:504	arg1	yield					506:510	Bacterial biomass, welan gum yield	477:510	yield	506:510	Bacterial biomass, welan gum yield, welan viscosity, molecular weight, monosaccharide composition, acyl content, and welan structure were analyzed.
28092912	7	68	theme	welan	838:842	arg1	concentration					844:856	a higher welan concentration	829:856	a higher welan concentration	829:856	Welan viscosity decreased at higher temperatures of 30-90°C, and it increased with a higher welan concentration.
29247412	4	0	theme	common	826:831	arg1	minerals					833:840	the PM2.5 common minerals	816:840	the PM2.5 common minerals	816:840	Meanwhile, as one of the PM2.5 common minerals, calcite fine particles were taken as the research object to explore the influence of microbial cells and extracellular polymers on its surface properties.
29247412	5	1	theme	crystal	1342:1348	arg1	form					1350:1353	crystal form	1342:1353	crystal form	1342:1353	High performance liquid chromatography (HPLC), inductively coupled plasma spectrometry (ICP), Zeta potential analysis, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), and scanning electron microscopy (SEM) were used to characterize the composition of EPS, the soluble ions, surface charge, surface groups, crystal form, and surface morphology of calcite residual solid after being treated by the bacterial cells and EPS.
29247412	10	2	theme	further	2180:2186	arg1	study					2188:2192	further study	2180:2192	further study	2180:2192	Experimental results also showed that the existence of the bacteria cells and EPS significantly affected the surface properties of calcite and provide a theoretical basis for the mechanism of PM fine particulate matter on human health impact for further study.
29247412	10	3	theme	bacteria	1993:2000	arg1	cells					2002:2006	the bacteria cells	1989:2006	the bacteria cells	1989:2006	Experimental results also showed that the existence of the bacteria cells and EPS significantly affected the surface properties of calcite and provide a theoretical basis for the mechanism of PM fine particulate matter on human health impact for further study.
29247412	3	4	theme	native	680:685	arg1	bacterium					692:700	a common native soil bacterium	671:700	a common native soil bacterium Bacillus mucitaginosus	671:723	To explore the process and the results of microbial cells and their extracellular polymeric substances (EPS) acting on the surface properties of minerals of PM2.5 through the metabolism, a common native soil bacterium Bacillus mucitaginosus with abundant extracellular polymers was chosen as the tested strain.
29247412	3	4	theme	native	680:685	arg1	PM2.5					641:645	PM2.5	641:645	PM2.5	641:645	To explore the process and the results of microbial cells and their extracellular polymeric substances (EPS) acting on the surface properties of minerals of PM2.5 through the metabolism, a common native soil bacterium Bacillus mucitaginosus with abundant extracellular polymers was chosen as the tested strain.
29247412	10	5	theme	theoretical	2087:2097	arg1	basis					2099:2103	a theoretical basis	2085:2103	a theoretical basis for the mechanism of PM fine particulate matter on human health impact for further study	2085:2192	Experimental results also showed that the existence of the bacteria cells and EPS significantly affected the surface properties of calcite and provide a theoretical basis for the mechanism of PM fine particulate matter on human health impact for further study.
29247412	1	6	theme	particulate	245:255	arg1	matter					257:262	fine particulate matter	240:262	fine particulate matter	240:262	Microorganism cells and spores are the main components of PM2.5 (fine particulate matter) as well as fine mineral particles.
29247412	1	6	theme	particulate	245:255	arg1	PM2.5					233:237	PM2.5	233:237	PM2.5 (fine particulate matter) as well as fine mineral particles	233:297	Microorganism cells and spores are the main components of PM2.5 (fine particulate matter) as well as fine mineral particles.
29247412	8	7	theme	negative	1770:1777	arg1	direction					1779:1787	the negative direction	1766:1787	the negative direction	1766:1787	Due to the adhesion of organic groups on the calcite surface, the surface potential shifted significantly in the negative direction and the solution pH was clearly increased.
29247412	5	8	theme	groups	1334:1339	arg1	composition					1272:1282	the composition	1268:1282	the composition of EPS, the soluble ions, surface charge, surface groups, crystal form, and surface morphology of calcite residual solid	1268:1403	High performance liquid chromatography (HPLC), inductively coupled plasma spectrometry (ICP), Zeta potential analysis, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), and scanning electron microscopy (SEM) were used to characterize the composition of EPS, the soluble ions, surface charge, surface groups, crystal form, and surface morphology of calcite residual solid after being treated by the bacterial cells and EPS.
29247412	1	9	theme	Microorganism	175:187	arg1	components					219:228	the main components	210:228	the main components of PM2.5 (fine particulate matter) as well as fine mineral particles	210:297	Microorganism cells and spores are the main components of PM2.5 (fine particulate matter) as well as fine mineral particles.
29247412	1	9	theme	Microorganism	175:187	arg1	spores					199:204	spores	199:204	spores	199:204	Microorganism cells and spores are the main components of PM2.5 (fine particulate matter) as well as fine mineral particles.
29247412	1	9	theme	Microorganism	175:187	arg1	cells					189:193	Microorganism cells	175:193	Microorganism cells	175:193	Microorganism cells and spores are the main components of PM2.5 (fine particulate matter) as well as fine mineral particles.
29247412	10	10	theme	calcite	2065:2071	arg1	properties					2051:2060	the surface properties	2039:2060	the surface properties of calcite	2039:2071	Experimental results also showed that the existence of the bacteria cells and EPS significantly affected the surface properties of calcite and provide a theoretical basis for the mechanism of PM fine particulate matter on human health impact for further study.
29247412	8	11	theme	solution	1797:1804	arg1	pH					1806:1807	the solution pH	1793:1807	the solution pH	1793:1807	Due to the adhesion of organic groups on the calcite surface, the surface potential shifted significantly in the negative direction and the solution pH was clearly increased.
29247412	3	12	theme	microbial	526:534	arg1	cells					536:540	microbial cells	526:540	microbial cells	526:540	To explore the process and the results of microbial cells and their extracellular polymeric substances (EPS) acting on the surface properties of minerals of PM2.5 through the metabolism, a common native soil bacterium Bacillus mucitaginosus with abundant extracellular polymers was chosen as the tested strain.
29247412	4	13	theme	research	884:891	arg1	particles					856:864	calcite fine particles	843:864	calcite fine particles	843:864	Meanwhile, as one of the PM2.5 common minerals, calcite fine particles were taken as the research object to explore the influence of microbial cells and extracellular polymers on its surface properties.
29247412	4	13	theme	research	884:891	arg1	object					893:898	the research object	880:898	the research object to explore the influence of microbial cells and extracellular polymers on its surface properties	880:995	Meanwhile, as one of the PM2.5 common minerals, calcite fine particles were taken as the research object to explore the influence of microbial cells and extracellular polymers on its surface properties.
29247412	4	13	theme	research	884:891	arg1	Meanwhile					795:803	Meanwhile	795:803	Meanwhile	795:803	Meanwhile, as one of the PM2.5 common minerals, calcite fine particles were taken as the research object to explore the influence of microbial cells and extracellular polymers on its surface properties.
29247412	0	14	theme	polymeric	121:129	arg1	EPS					143:145	EPS	143:145	EPS	143:145	Surface properties of PM2.5 calcite fine particulate matter in the presence of same size bacterial cells and exocellular polymeric substances (EPS) of Bacillus mucitaginosus.
29247412	0	14	theme	polymeric	121:129	arg1	substances					131:140	exocellular polymeric substances	109:140	exocellular polymeric substances (EPS)	109:146	Surface properties of PM2.5 calcite fine particulate matter in the presence of same size bacterial cells and exocellular polymeric substances (EPS) of Bacillus mucitaginosus.
29247412	3	15	theme	substances	576:585	arg1	results					515:521	the results	511:521	the results of microbial cells and their extracellular polymeric substances (EPS) acting on the surface properties of minerals of PM2.5 through the metabolism, a common native soil bacterium Bacillus mucitaginosus with abundant extracellular polymers	511:760	To explore the process and the results of microbial cells and their extracellular polymeric substances (EPS) acting on the surface properties of minerals of PM2.5 through the metabolism, a common native soil bacterium Bacillus mucitaginosus with abundant extracellular polymers was chosen as the tested strain.
29247412	3	15	theme	substances	576:585	arg1	strain					787:792	the tested strain	776:792	the tested strain	776:792	To explore the process and the results of microbial cells and their extracellular polymeric substances (EPS) acting on the surface properties of minerals of PM2.5 through the metabolism, a common native soil bacterium Bacillus mucitaginosus with abundant extracellular polymers was chosen as the tested strain.
29247412	6	16	theme	mucitaginosus	1492:1504	arg1	EPS					1482:1484	the EPS	1478:1484	the EPS of B. mucitaginosus mainly consisted of protein and polysaccharides	1478:1552	The results revealed the EPS of B. mucitaginosus mainly consisted of protein and polysaccharides.
29247412	4	17	theme	fine	851:854	arg1	particles					856:864	calcite fine particles	843:864	calcite fine particles	843:864	Meanwhile, as one of the PM2.5 common minerals, calcite fine particles were taken as the research object to explore the influence of microbial cells and extracellular polymers on its surface properties.
29247412	4	17	theme	fine	851:854	arg1	minerals					833:840	the PM2.5 common minerals	816:840	the PM2.5 common minerals	816:840	Meanwhile, as one of the PM2.5 common minerals, calcite fine particles were taken as the research object to explore the influence of microbial cells and extracellular polymers on its surface properties.
29247412	4	17	theme	fine	851:854	arg1	object					893:898	the research object	880:898	the research object to explore the influence of microbial cells and extracellular polymers on its surface properties	880:995	Meanwhile, as one of the PM2.5 common minerals, calcite fine particles were taken as the research object to explore the influence of microbial cells and extracellular polymers on its surface properties.
29247412	4	17	theme	fine	851:854	arg1	Meanwhile					795:803	Meanwhile	795:803	Meanwhile	795:803	Meanwhile, as one of the PM2.5 common minerals, calcite fine particles were taken as the research object to explore the influence of microbial cells and extracellular polymers on its surface properties.
29247412	4	17	theme	fine	851:854	arg1	one					809:811	one	809:811	one	809:811	Meanwhile, as one of the PM2.5 common minerals, calcite fine particles were taken as the research object to explore the influence of microbial cells and extracellular polymers on its surface properties.
29247412	9	18	theme	calcite	1850:1856	arg1	surface					1858:1864	calcite surface	1850:1864	calcite surface	1850:1864	The morphology of calcite surface was significantly changed after dissolution and re-crystallization.
29247412	3	19	theme	abundant	730:737	arg1	polymers					753:760	abundant extracellular polymers	730:760	abundant extracellular polymers	730:760	To explore the process and the results of microbial cells and their extracellular polymeric substances (EPS) acting on the surface properties of minerals of PM2.5 through the metabolism, a common native soil bacterium Bacillus mucitaginosus with abundant extracellular polymers was chosen as the tested strain.
29247412	5	20	theme	High	998:1001	arg1	HPLC					1038:1041	HPLC	1038:1041	HPLC	1038:1041	High performance liquid chromatography (HPLC), inductively coupled plasma spectrometry (ICP), Zeta potential analysis, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), and scanning electron microscopy (SEM) were used to characterize the composition of EPS, the soluble ions, surface charge, surface groups, crystal form, and surface morphology of calcite residual solid after being treated by the bacterial cells and EPS.
29247412	5	20	theme	High	998:1001	arg1	chromatography					1022:1035	High performance liquid chromatography	998:1035	High performance liquid chromatography (HPLC)	998:1042	High performance liquid chromatography (HPLC), inductively coupled plasma spectrometry (ICP), Zeta potential analysis, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), and scanning electron microscopy (SEM) were used to characterize the composition of EPS, the soluble ions, surface charge, surface groups, crystal form, and surface morphology of calcite residual solid after being treated by the bacterial cells and EPS.
29247412	3	21	theme	extracellular	552:564	arg1	EPS					588:590	EPS	588:590	EPS	588:590	To explore the process and the results of microbial cells and their extracellular polymeric substances (EPS) acting on the surface properties of minerals of PM2.5 through the metabolism, a common native soil bacterium Bacillus mucitaginosus with abundant extracellular polymers was chosen as the tested strain.
29247412	3	21	theme	extracellular	552:564	arg1	substances					576:585	their extracellular polymeric substances	546:585	their extracellular polymeric substances (EPS) acting on the surface properties of minerals of PM2.5 through the metabolism, a common native soil bacterium Bacillus mucitaginosus with abundant extracellular polymers	546:760	To explore the process and the results of microbial cells and their extracellular polymeric substances (EPS) acting on the surface properties of minerals of PM2.5 through the metabolism, a common native soil bacterium Bacillus mucitaginosus with abundant extracellular polymers was chosen as the tested strain.
29247412	7	22	theme	calcium	1643:1649	arg1	ions					1651:1654	calcium ions	1643:1654	calcium ions	1643:1654	Both the whole cell and its EPS could promote the dissolution of calcite particles into calcium ions.
29247412	10	23	from	matter	2146:2151	arg1	impact					2169:2174	human health impact	2156:2174	human health impact	2156:2174	Experimental results also showed that the existence of the bacteria cells and EPS significantly affected the surface properties of calcite and provide a theoretical basis for the mechanism of PM fine particulate matter on human health impact for further study.
29247412	10	24	theme	Experimental	1934:1945	arg1	results					1947:1953	Experimental results	1934:1953	Experimental results	1934:1953	Experimental results also showed that the existence of the bacteria cells and EPS significantly affected the surface properties of calcite and provide a theoretical basis for the mechanism of PM fine particulate matter on human health impact for further study.
29247412	5	25	theme	liquid	1015:1020	arg1	HPLC					1038:1041	HPLC	1038:1041	HPLC	1038:1041	High performance liquid chromatography (HPLC), inductively coupled plasma spectrometry (ICP), Zeta potential analysis, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), and scanning electron microscopy (SEM) were used to characterize the composition of EPS, the soluble ions, surface charge, surface groups, crystal form, and surface morphology of calcite residual solid after being treated by the bacterial cells and EPS.
29247412	5	25	theme	liquid	1015:1020	arg1	chromatography					1022:1035	High performance liquid chromatography	998:1035	High performance liquid chromatography (HPLC)	998:1042	High performance liquid chromatography (HPLC), inductively coupled plasma spectrometry (ICP), Zeta potential analysis, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), and scanning electron microscopy (SEM) were used to characterize the composition of EPS, the soluble ions, surface charge, surface groups, crystal form, and surface morphology of calcite residual solid after being treated by the bacterial cells and EPS.
29247412	7	26	theme	particles	1628:1636	arg1	dissolution					1605:1615	the dissolution	1601:1615	the dissolution of calcite particles into calcium ions	1601:1654	Both the whole cell and its EPS could promote the dissolution of calcite particles into calcium ions.
29247412	8	27	theme	groups	1688:1693	arg1	adhesion					1668:1675	the adhesion	1664:1675	the adhesion of organic groups on the calcite surface	1664:1716	Due to the adhesion of organic groups on the calcite surface, the surface potential shifted significantly in the negative direction and the solution pH was clearly increased.
29247412	4	28	theme	cells	938:942	arg1	influence					915:923	the influence	911:923	the influence of microbial cells and extracellular polymers on its surface properties	911:995	Meanwhile, as one of the PM2.5 common minerals, calcite fine particles were taken as the research object to explore the influence of microbial cells and extracellular polymers on its surface properties.
29247412	10	29	from	impact	2169:2174	arg1	mechanism					2113:2121	the mechanism	2109:2121	the mechanism of PM fine particulate matter on human health impact	2109:2174	Experimental results also showed that the existence of the bacteria cells and EPS significantly affected the surface properties of calcite and provide a theoretical basis for the mechanism of PM fine particulate matter on human health impact for further study.
29247412	5	30	used	used	1247:1250	arg2	XRD					1197:1199	XRD	1197:1199	XRD	1197:1199	High performance liquid chromatography (HPLC), inductively coupled plasma spectrometry (ICP), Zeta potential analysis, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), and scanning electron microscopy (SEM) were used to characterize the composition of EPS, the soluble ions, surface charge, surface groups, crystal form, and surface morphology of calcite residual solid after being treated by the bacterial cells and EPS.
29247412	5	30	used	used	1247:1250	arg2	microscopy					1225:1234	scanning electron microscopy	1207:1234	scanning electron microscopy (SEM)	1207:1240	High performance liquid chromatography (HPLC), inductively coupled plasma spectrometry (ICP), Zeta potential analysis, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), and scanning electron microscopy (SEM) were used to characterize the composition of EPS, the soluble ions, surface charge, surface groups, crystal form, and surface morphology of calcite residual solid after being treated by the bacterial cells and EPS.
29247412	5	30	used	used	1247:1250	arg2	spectrometry					1072:1083	inductively coupled plasma spectrometry	1045:1083	inductively coupled plasma spectrometry (ICP)	1045:1089	High performance liquid chromatography (HPLC), inductively coupled plasma spectrometry (ICP), Zeta potential analysis, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), and scanning electron microscopy (SEM) were used to characterize the composition of EPS, the soluble ions, surface charge, surface groups, crystal form, and surface morphology of calcite residual solid after being treated by the bacterial cells and EPS.
29247412	5	30	used	used	1247:1250	arg2	SEM					1237:1239	SEM	1237:1239	SEM	1237:1239	High performance liquid chromatography (HPLC), inductively coupled plasma spectrometry (ICP), Zeta potential analysis, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), and scanning electron microscopy (SEM) were used to characterize the composition of EPS, the soluble ions, surface charge, surface groups, crystal form, and surface morphology of calcite residual solid after being treated by the bacterial cells and EPS.
29247412	5	30	used	used	1247:1250	arg2	ICP					1086:1088	ICP	1086:1088	ICP	1086:1088	High performance liquid chromatography (HPLC), inductively coupled plasma spectrometry (ICP), Zeta potential analysis, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), and scanning electron microscopy (SEM) were used to characterize the composition of EPS, the soluble ions, surface charge, surface groups, crystal form, and surface morphology of calcite residual solid after being treated by the bacterial cells and EPS.
29247412	5	30	used	used	1247:1250	arg2	HPLC					1038:1041	HPLC	1038:1041	HPLC	1038:1041	High performance liquid chromatography (HPLC), inductively coupled plasma spectrometry (ICP), Zeta potential analysis, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), and scanning electron microscopy (SEM) were used to characterize the composition of EPS, the soluble ions, surface charge, surface groups, crystal form, and surface morphology of calcite residual solid after being treated by the bacterial cells and EPS.
29247412	5	30	used	used	1247:1250	arg2	analysis					1107:1114	Zeta potential analysis	1092:1114	Zeta potential analysis	1092:1114	High performance liquid chromatography (HPLC), inductively coupled plasma spectrometry (ICP), Zeta potential analysis, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), and scanning electron microscopy (SEM) were used to characterize the composition of EPS, the soluble ions, surface charge, surface groups, crystal form, and surface morphology of calcite residual solid after being treated by the bacterial cells and EPS.
29247412	5	30	used	used	1247:1250	arg2	FTIR					1158:1161	FTIR	1158:1161	FTIR	1158:1161	High performance liquid chromatography (HPLC), inductively coupled plasma spectrometry (ICP), Zeta potential analysis, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), and scanning electron microscopy (SEM) were used to characterize the composition of EPS, the soluble ions, surface charge, surface groups, crystal form, and surface morphology of calcite residual solid after being treated by the bacterial cells and EPS.
29247412	5	30	used	used	1247:1250	arg2	spectroscopy					1183:1194	X-ray diffraction spectroscopy	1165:1194	X-ray diffraction spectroscopy (XRD)	1165:1200	High performance liquid chromatography (HPLC), inductively coupled plasma spectrometry (ICP), Zeta potential analysis, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), and scanning electron microscopy (SEM) were used to characterize the composition of EPS, the soluble ions, surface charge, surface groups, crystal form, and surface morphology of calcite residual solid after being treated by the bacterial cells and EPS.
29247412	5	30	used	used	1247:1250	arg2	spectroscopy					1144:1155	Fourier-transform infrared spectroscopy	1117:1155	Fourier-transform infrared spectroscopy (FTIR)	1117:1162	High performance liquid chromatography (HPLC), inductively coupled plasma spectrometry (ICP), Zeta potential analysis, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), and scanning electron microscopy (SEM) were used to characterize the composition of EPS, the soluble ions, surface charge, surface groups, crystal form, and surface morphology of calcite residual solid after being treated by the bacterial cells and EPS.
29247412	5	30	used	used	1247:1250	arg2	chromatography					1022:1035	High performance liquid chromatography	998:1035	High performance liquid chromatography (HPLC)	998:1042	High performance liquid chromatography (HPLC), inductively coupled plasma spectrometry (ICP), Zeta potential analysis, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), and scanning electron microscopy (SEM) were used to characterize the composition of EPS, the soluble ions, surface charge, surface groups, crystal form, and surface morphology of calcite residual solid after being treated by the bacterial cells and EPS.
29247412	5	31	theme	scanning	1207:1214	arg1	microscopy					1225:1234	scanning electron microscopy	1207:1234	scanning electron microscopy (SEM)	1207:1240	High performance liquid chromatography (HPLC), inductively coupled plasma spectrometry (ICP), Zeta potential analysis, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), and scanning electron microscopy (SEM) were used to characterize the composition of EPS, the soluble ions, surface charge, surface groups, crystal form, and surface morphology of calcite residual solid after being treated by the bacterial cells and EPS.
29247412	5	31	theme	scanning	1207:1214	arg1	SEM					1237:1239	SEM	1237:1239	SEM	1237:1239	High performance liquid chromatography (HPLC), inductively coupled plasma spectrometry (ICP), Zeta potential analysis, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), and scanning electron microscopy (SEM) were used to characterize the composition of EPS, the soluble ions, surface charge, surface groups, crystal form, and surface morphology of calcite residual solid after being treated by the bacterial cells and EPS.
29247412	0	32	theme	same	79:82	arg1	cells					99:103	same size bacterial cells	79:103	same size bacterial cells	79:103	Surface properties of PM2.5 calcite fine particulate matter in the presence of same size bacterial cells and exocellular polymeric substances (EPS) of Bacillus mucitaginosus.
29247412	5	33	theme	surface	1360:1366	arg1	morphology					1368:1377	surface morphology	1360:1377	surface morphology	1360:1377	High performance liquid chromatography (HPLC), inductively coupled plasma spectrometry (ICP), Zeta potential analysis, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), and scanning electron microscopy (SEM) were used to characterize the composition of EPS, the soluble ions, surface charge, surface groups, crystal form, and surface morphology of calcite residual solid after being treated by the bacterial cells and EPS.
29247412	10	34	theme	PM	2126:2127	arg1	matter					2146:2151	PM fine particulate matter	2126:2151	PM fine particulate matter on human health impact	2126:2174	Experimental results also showed that the existence of the bacteria cells and EPS significantly affected the surface properties of calcite and provide a theoretical basis for the mechanism of PM fine particulate matter on human health impact for further study.
29247412	5	35	theme	X-ray	1165:1169	arg1	spectroscopy					1183:1194	X-ray diffraction spectroscopy	1165:1194	X-ray diffraction spectroscopy (XRD)	1165:1200	High performance liquid chromatography (HPLC), inductively coupled plasma spectrometry (ICP), Zeta potential analysis, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), and scanning electron microscopy (SEM) were used to characterize the composition of EPS, the soluble ions, surface charge, surface groups, crystal form, and surface morphology of calcite residual solid after being treated by the bacterial cells and EPS.
29247412	5	35	theme	X-ray	1165:1169	arg1	XRD					1197:1199	XRD	1197:1199	XRD	1197:1199	High performance liquid chromatography (HPLC), inductively coupled plasma spectrometry (ICP), Zeta potential analysis, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), and scanning electron microscopy (SEM) were used to characterize the composition of EPS, the soluble ions, surface charge, surface groups, crystal form, and surface morphology of calcite residual solid after being treated by the bacterial cells and EPS.
29247412	5	36	theme	plasma	1065:1070	arg1	ICP					1086:1088	ICP	1086:1088	ICP	1086:1088	High performance liquid chromatography (HPLC), inductively coupled plasma spectrometry (ICP), Zeta potential analysis, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), and scanning electron microscopy (SEM) were used to characterize the composition of EPS, the soluble ions, surface charge, surface groups, crystal form, and surface morphology of calcite residual solid after being treated by the bacterial cells and EPS.
29247412	5	36	theme	plasma	1065:1070	arg1	spectrometry					1072:1083	inductively coupled plasma spectrometry	1045:1083	inductively coupled plasma spectrometry (ICP)	1045:1089	High performance liquid chromatography (HPLC), inductively coupled plasma spectrometry (ICP), Zeta potential analysis, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), and scanning electron microscopy (SEM) were used to characterize the composition of EPS, the soluble ions, surface charge, surface groups, crystal form, and surface morphology of calcite residual solid after being treated by the bacterial cells and EPS.
29247412	4	37	theme	extracellular	948:960	arg1	polymers					962:969	extracellular polymers	948:969	extracellular polymers	948:969	Meanwhile, as one of the PM2.5 common minerals, calcite fine particles were taken as the research object to explore the influence of microbial cells and extracellular polymers on its surface properties.
29247412	4	38	theme	surface	978:984	arg1	properties					986:995	its surface properties	974:995	its surface properties	974:995	Meanwhile, as one of the PM2.5 common minerals, calcite fine particles were taken as the research object to explore the influence of microbial cells and extracellular polymers on its surface properties.
29247412	10	39	theme	health	2162:2167	arg1	impact					2169:2174	human health impact	2156:2174	human health impact	2156:2174	Experimental results also showed that the existence of the bacteria cells and EPS significantly affected the surface properties of calcite and provide a theoretical basis for the mechanism of PM fine particulate matter on human health impact for further study.
29247412	0	40	theme	Surface	0:6	arg1	properties					8:17	Surface properties	0:17	Surface properties of PM2.5	0:26	Surface properties of PM2.5 calcite fine particulate matter in the presence of same size bacterial cells and exocellular polymeric substances (EPS) of Bacillus mucitaginosus.
29247412	5	41	theme	Fourier-transform	1117:1133	arg1	FTIR					1158:1161	FTIR	1158:1161	FTIR	1158:1161	High performance liquid chromatography (HPLC), inductively coupled plasma spectrometry (ICP), Zeta potential analysis, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), and scanning electron microscopy (SEM) were used to characterize the composition of EPS, the soluble ions, surface charge, surface groups, crystal form, and surface morphology of calcite residual solid after being treated by the bacterial cells and EPS.
29247412	5	41	theme	Fourier-transform	1117:1133	arg1	spectroscopy					1144:1155	Fourier-transform infrared spectroscopy	1117:1155	Fourier-transform infrared spectroscopy (FTIR)	1117:1162	High performance liquid chromatography (HPLC), inductively coupled plasma spectrometry (ICP), Zeta potential analysis, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), and scanning electron microscopy (SEM) were used to characterize the composition of EPS, the soluble ions, surface charge, surface groups, crystal form, and surface morphology of calcite residual solid after being treated by the bacterial cells and EPS.
29247412	2	42	theme	charge	391:396	arg1	neutralization					398:411	charge neutralization	391:411	charge neutralization	391:411	In the microscopic system, the microorganisms will affect the minerals through attachment, charge neutralization, and dissolution related to the cell surface structure and metabolite.
29247412	2	43	theme	cell	445:448	arg1	surface					450:456	the cell surface structure and metabolite	441:481	the cell surface structure and metabolite	441:481	In the microscopic system, the microorganisms will affect the minerals through attachment, charge neutralization, and dissolution related to the cell surface structure and metabolite.
29247412	5	44	theme	Zeta	1092:1095	arg1	analysis					1107:1114	Zeta potential analysis	1092:1114	Zeta potential analysis	1092:1114	High performance liquid chromatography (HPLC), inductively coupled plasma spectrometry (ICP), Zeta potential analysis, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), and scanning electron microscopy (SEM) were used to characterize the composition of EPS, the soluble ions, surface charge, surface groups, crystal form, and surface morphology of calcite residual solid after being treated by the bacterial cells and EPS.
29247412	2	45	theme	microscopic	307:317	arg1	system					319:324	the microscopic system	303:324	the microscopic system	303:324	In the microscopic system, the microorganisms will affect the minerals through attachment, charge neutralization, and dissolution related to the cell surface structure and metabolite.
29247412	0	46	theme	particulate	41:51	arg1	matter					53:58	fine particulate matter	36:58	fine particulate matter in the presence of same size bacterial cells and exocellular polymeric substances (EPS) of Bacillus mucitaginosus	36:172	Surface properties of PM2.5 calcite fine particulate matter in the presence of same size bacterial cells and exocellular polymeric substances (EPS) of Bacillus mucitaginosus.
29247412	1	47	theme	mineral	281:287	arg1	particles					289:297	fine mineral particles	276:297	PM2.5 (fine particulate matter) as well as fine mineral particles	233:297	Microorganism cells and spores are the main components of PM2.5 (fine particulate matter) as well as fine mineral particles.
29247412	2	48	dep	surface	450:456	arg1	metabolite					472:481	metabolite	472:481	metabolite	472:481	In the microscopic system, the microorganisms will affect the minerals through attachment, charge neutralization, and dissolution related to the cell surface structure and metabolite.
29247412	2	48	dep	surface	450:456	arg1	structure					458:466	structure	458:466	structure	458:466	In the microscopic system, the microorganisms will affect the minerals through attachment, charge neutralization, and dissolution related to the cell surface structure and metabolite.
29247412	3	49	theme	PM2.5	641:645	arg1	minerals					629:636	minerals	629:636	minerals of PM2.5 through the metabolism, a common native soil bacterium Bacillus mucitaginosus with abundant extracellular polymers	629:760	To explore the process and the results of microbial cells and their extracellular polymeric substances (EPS) acting on the surface properties of minerals of PM2.5 through the metabolism, a common native soil bacterium Bacillus mucitaginosus with abundant extracellular polymers was chosen as the tested strain.
29247412	5	50	theme	charge	1318:1323	arg1	composition					1272:1282	the composition	1268:1282	the composition of EPS, the soluble ions, surface charge, surface groups, crystal form, and surface morphology of calcite residual solid	1268:1403	High performance liquid chromatography (HPLC), inductively coupled plasma spectrometry (ICP), Zeta potential analysis, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), and scanning electron microscopy (SEM) were used to characterize the composition of EPS, the soluble ions, surface charge, surface groups, crystal form, and surface morphology of calcite residual solid after being treated by the bacterial cells and EPS.
29247412	5	51	theme	diffraction	1171:1181	arg1	spectroscopy					1183:1194	X-ray diffraction spectroscopy	1165:1194	X-ray diffraction spectroscopy (XRD)	1165:1200	High performance liquid chromatography (HPLC), inductively coupled plasma spectrometry (ICP), Zeta potential analysis, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), and scanning electron microscopy (SEM) were used to characterize the composition of EPS, the soluble ions, surface charge, surface groups, crystal form, and surface morphology of calcite residual solid after being treated by the bacterial cells and EPS.
29247412	5	51	theme	diffraction	1171:1181	arg1	XRD					1197:1199	XRD	1197:1199	XRD	1197:1199	High performance liquid chromatography (HPLC), inductively coupled plasma spectrometry (ICP), Zeta potential analysis, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), and scanning electron microscopy (SEM) were used to characterize the composition of EPS, the soluble ions, surface charge, surface groups, crystal form, and surface morphology of calcite residual solid after being treated by the bacterial cells and EPS.
29247412	3	52	theme	minerals	629:636	arg1	properties					615:624	the surface properties	603:624	the surface properties of minerals of PM2.5 through the metabolism, a common native soil bacterium Bacillus mucitaginosus with abundant extracellular polymers	603:760	To explore the process and the results of microbial cells and their extracellular polymeric substances (EPS) acting on the surface properties of minerals of PM2.5 through the metabolism, a common native soil bacterium Bacillus mucitaginosus with abundant extracellular polymers was chosen as the tested strain.
29247412	10	53	theme	EPS	2012:2014	arg1	existence					1976:1984	the existence	1972:1984	the existence of the bacteria cells and EPS	1972:2014	Experimental results also showed that the existence of the bacteria cells and EPS significantly affected the surface properties of calcite and provide a theoretical basis for the mechanism of PM fine particulate matter on human health impact for further study.
29247412	5	54	theme	potential	1097:1105	arg1	analysis					1107:1114	Zeta potential analysis	1092:1114	Zeta potential analysis	1092:1114	High performance liquid chromatography (HPLC), inductively coupled plasma spectrometry (ICP), Zeta potential analysis, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), and scanning electron microscopy (SEM) were used to characterize the composition of EPS, the soluble ions, surface charge, surface groups, crystal form, and surface morphology of calcite residual solid after being treated by the bacterial cells and EPS.
29247412	0	55	theme	mucitaginosus	160:172	arg1	presence					67:74	the presence	63:74	the presence of same size bacterial cells and exocellular polymeric substances (EPS) of Bacillus mucitaginosus	63:172	Surface properties of PM2.5 calcite fine particulate matter in the presence of same size bacterial cells and exocellular polymeric substances (EPS) of Bacillus mucitaginosus.
29247412	5	56	theme	soluble	1296:1302	arg1	ions					1304:1307	the soluble ions	1292:1307	the soluble ions	1292:1307	High performance liquid chromatography (HPLC), inductively coupled plasma spectrometry (ICP), Zeta potential analysis, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), and scanning electron microscopy (SEM) were used to characterize the composition of EPS, the soluble ions, surface charge, surface groups, crystal form, and surface morphology of calcite residual solid after being treated by the bacterial cells and EPS.
29247412	1	57	theme	PM2.5	233:237	arg1	components					219:228	the main components	210:228	the main components of PM2.5 (fine particulate matter) as well as fine mineral particles	210:297	Microorganism cells and spores are the main components of PM2.5 (fine particulate matter) as well as fine mineral particles.
29247412	1	57	theme	PM2.5	233:237	arg1	spores					199:204	spores	199:204	spores	199:204	Microorganism cells and spores are the main components of PM2.5 (fine particulate matter) as well as fine mineral particles.
29247412	1	57	theme	PM2.5	233:237	arg1	cells					189:193	Microorganism cells	175:193	Microorganism cells	175:193	Microorganism cells and spores are the main components of PM2.5 (fine particulate matter) as well as fine mineral particles.
29247412	10	58	theme	cells	2002:2006	arg1	existence					1976:1984	the existence	1972:1984	the existence of the bacteria cells and EPS	1972:2014	Experimental results also showed that the existence of the bacteria cells and EPS significantly affected the surface properties of calcite and provide a theoretical basis for the mechanism of PM fine particulate matter on human health impact for further study.
29247412	5	59	theme	form	1350:1353	arg1	composition					1272:1282	the composition	1268:1282	the composition of EPS, the soluble ions, surface charge, surface groups, crystal form, and surface morphology of calcite residual solid	1268:1403	High performance liquid chromatography (HPLC), inductively coupled plasma spectrometry (ICP), Zeta potential analysis, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), and scanning electron microscopy (SEM) were used to characterize the composition of EPS, the soluble ions, surface charge, surface groups, crystal form, and surface morphology of calcite residual solid after being treated by the bacterial cells and EPS.
29247412	1	60	theme	fine	240:243	arg1	matter					257:262	fine particulate matter	240:262	fine particulate matter	240:262	Microorganism cells and spores are the main components of PM2.5 (fine particulate matter) as well as fine mineral particles.
29247412	1	60	theme	fine	240:243	arg1	PM2.5					233:237	PM2.5	233:237	PM2.5 (fine particulate matter) as well as fine mineral particles	233:297	Microorganism cells and spores are the main components of PM2.5 (fine particulate matter) as well as fine mineral particles.
29247412	4	61	theme	minerals	833:840	arg1	particles					856:864	calcite fine particles	843:864	calcite fine particles	843:864	Meanwhile, as one of the PM2.5 common minerals, calcite fine particles were taken as the research object to explore the influence of microbial cells and extracellular polymers on its surface properties.
29247412	4	61	theme	minerals	833:840	arg1	Meanwhile					795:803	Meanwhile	795:803	Meanwhile	795:803	Meanwhile, as one of the PM2.5 common minerals, calcite fine particles were taken as the research object to explore the influence of microbial cells and extracellular polymers on its surface properties.
29247412	4	61	theme	minerals	833:840	arg1	minerals					833:840	the PM2.5 common minerals	816:840	the PM2.5 common minerals	816:840	Meanwhile, as one of the PM2.5 common minerals, calcite fine particles were taken as the research object to explore the influence of microbial cells and extracellular polymers on its surface properties.
29247412	4	61	theme	minerals	833:840	arg1	one					809:811	one	809:811	one	809:811	Meanwhile, as one of the PM2.5 common minerals, calcite fine particles were taken as the research object to explore the influence of microbial cells and extracellular polymers on its surface properties.
29247412	10	62	theme	surface	2043:2049	arg1	properties					2051:2060	the surface properties	2039:2060	the surface properties of calcite	2039:2071	Experimental results also showed that the existence of the bacteria cells and EPS significantly affected the surface properties of calcite and provide a theoretical basis for the mechanism of PM fine particulate matter on human health impact for further study.
29247412	3	63	theme	soil	687:690	arg1	bacterium					692:700	a common native soil bacterium	671:700	a common native soil bacterium Bacillus mucitaginosus	671:723	To explore the process and the results of microbial cells and their extracellular polymeric substances (EPS) acting on the surface properties of minerals of PM2.5 through the metabolism, a common native soil bacterium Bacillus mucitaginosus with abundant extracellular polymers was chosen as the tested strain.
29247412	3	63	theme	soil	687:690	arg1	PM2.5					641:645	PM2.5	641:645	PM2.5	641:645	To explore the process and the results of microbial cells and their extracellular polymeric substances (EPS) acting on the surface properties of minerals of PM2.5 through the metabolism, a common native soil bacterium Bacillus mucitaginosus with abundant extracellular polymers was chosen as the tested strain.
29247412	3	64	with	minerals	629:636	arg1	polymers					753:760	abundant extracellular polymers	730:760	abundant extracellular polymers	730:760	To explore the process and the results of microbial cells and their extracellular polymeric substances (EPS) acting on the surface properties of minerals of PM2.5 through the metabolism, a common native soil bacterium Bacillus mucitaginosus with abundant extracellular polymers was chosen as the tested strain.
29247412	2	65	theme	related	430:436	arg1	dissolution					418:428	dissolution	418:428	dissolution related to the cell surface structure and metabolite	418:481	In the microscopic system, the microorganisms will affect the minerals through attachment, charge neutralization, and dissolution related to the cell surface structure and metabolite.
29247412	3	66	theme	common	673:678	arg1	bacterium					692:700	a common native soil bacterium	671:700	a common native soil bacterium Bacillus mucitaginosus	671:723	To explore the process and the results of microbial cells and their extracellular polymeric substances (EPS) acting on the surface properties of minerals of PM2.5 through the metabolism, a common native soil bacterium Bacillus mucitaginosus with abundant extracellular polymers was chosen as the tested strain.
29247412	3	66	theme	common	673:678	arg1	PM2.5					641:645	PM2.5	641:645	PM2.5	641:645	To explore the process and the results of microbial cells and their extracellular polymeric substances (EPS) acting on the surface properties of minerals of PM2.5 through the metabolism, a common native soil bacterium Bacillus mucitaginosus with abundant extracellular polymers was chosen as the tested strain.
29247412	5	67	theme	surface	1326:1332	arg1	groups					1334:1339	surface groups	1326:1339	surface groups	1326:1339	High performance liquid chromatography (HPLC), inductively coupled plasma spectrometry (ICP), Zeta potential analysis, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), and scanning electron microscopy (SEM) were used to characterize the composition of EPS, the soluble ions, surface charge, surface groups, crystal form, and surface morphology of calcite residual solid after being treated by the bacterial cells and EPS.
29247412	3	68	theme	cells	536:540	arg1	results					515:521	the results	511:521	the results of microbial cells and their extracellular polymeric substances (EPS) acting on the surface properties of minerals of PM2.5 through the metabolism, a common native soil bacterium Bacillus mucitaginosus with abundant extracellular polymers	511:760	To explore the process and the results of microbial cells and their extracellular polymeric substances (EPS) acting on the surface properties of minerals of PM2.5 through the metabolism, a common native soil bacterium Bacillus mucitaginosus with abundant extracellular polymers was chosen as the tested strain.
29247412	3	68	theme	cells	536:540	arg1	strain					787:792	the tested strain	776:792	the tested strain	776:792	To explore the process and the results of microbial cells and their extracellular polymeric substances (EPS) acting on the surface properties of minerals of PM2.5 through the metabolism, a common native soil bacterium Bacillus mucitaginosus with abundant extracellular polymers was chosen as the tested strain.
29247412	0	69	theme	exocellular	109:119	arg1	EPS					143:145	EPS	143:145	EPS	143:145	Surface properties of PM2.5 calcite fine particulate matter in the presence of same size bacterial cells and exocellular polymeric substances (EPS) of Bacillus mucitaginosus.
29247412	0	69	theme	exocellular	109:119	arg1	substances					131:140	exocellular polymeric substances	109:140	exocellular polymeric substances (EPS)	109:146	Surface properties of PM2.5 calcite fine particulate matter in the presence of same size bacterial cells and exocellular polymeric substances (EPS) of Bacillus mucitaginosus.
29247412	3	70	theme	Bacillus	702:709	arg1	bacterium					692:700	a common native soil bacterium	671:700	a common native soil bacterium Bacillus mucitaginosus	671:723	To explore the process and the results of microbial cells and their extracellular polymeric substances (EPS) acting on the surface properties of minerals of PM2.5 through the metabolism, a common native soil bacterium Bacillus mucitaginosus with abundant extracellular polymers was chosen as the tested strain.
29247412	3	70	theme	Bacillus	702:709	arg1	PM2.5					641:645	PM2.5	641:645	PM2.5	641:645	To explore the process and the results of microbial cells and their extracellular polymeric substances (EPS) acting on the surface properties of minerals of PM2.5 through the metabolism, a common native soil bacterium Bacillus mucitaginosus with abundant extracellular polymers was chosen as the tested strain.
29247412	0	71	theme	substances	131:140	arg1	presence					67:74	the presence	63:74	the presence of same size bacterial cells and exocellular polymeric substances (EPS) of Bacillus mucitaginosus	63:172	Surface properties of PM2.5 calcite fine particulate matter in the presence of same size bacterial cells and exocellular polymeric substances (EPS) of Bacillus mucitaginosus.
29247412	3	72	theme	polymeric	566:574	arg1	EPS					588:590	EPS	588:590	EPS	588:590	To explore the process and the results of microbial cells and their extracellular polymeric substances (EPS) acting on the surface properties of minerals of PM2.5 through the metabolism, a common native soil bacterium Bacillus mucitaginosus with abundant extracellular polymers was chosen as the tested strain.
29247412	3	72	theme	polymeric	566:574	arg1	substances					576:585	their extracellular polymeric substances	546:585	their extracellular polymeric substances (EPS) acting on the surface properties of minerals of PM2.5 through the metabolism, a common native soil bacterium Bacillus mucitaginosus with abundant extracellular polymers	546:760	To explore the process and the results of microbial cells and their extracellular polymeric substances (EPS) acting on the surface properties of minerals of PM2.5 through the metabolism, a common native soil bacterium Bacillus mucitaginosus with abundant extracellular polymers was chosen as the tested strain.
29247412	8	73	from	adhesion	1668:1675	arg1	surface					1710:1716	the calcite surface	1698:1716	the calcite surface	1698:1716	Due to the adhesion of organic groups on the calcite surface, the surface potential shifted significantly in the negative direction and the solution pH was clearly increased.
29247412	4	74	theme	calcite	843:849	arg1	particles					856:864	calcite fine particles	843:864	calcite fine particles	843:864	Meanwhile, as one of the PM2.5 common minerals, calcite fine particles were taken as the research object to explore the influence of microbial cells and extracellular polymers on its surface properties.
29247412	4	74	theme	calcite	843:849	arg1	minerals					833:840	the PM2.5 common minerals	816:840	the PM2.5 common minerals	816:840	Meanwhile, as one of the PM2.5 common minerals, calcite fine particles were taken as the research object to explore the influence of microbial cells and extracellular polymers on its surface properties.
29247412	4	74	theme	calcite	843:849	arg1	object					893:898	the research object	880:898	the research object to explore the influence of microbial cells and extracellular polymers on its surface properties	880:995	Meanwhile, as one of the PM2.5 common minerals, calcite fine particles were taken as the research object to explore the influence of microbial cells and extracellular polymers on its surface properties.
29247412	4	74	theme	calcite	843:849	arg1	Meanwhile					795:803	Meanwhile	795:803	Meanwhile	795:803	Meanwhile, as one of the PM2.5 common minerals, calcite fine particles were taken as the research object to explore the influence of microbial cells and extracellular polymers on its surface properties.
29247412	4	74	theme	calcite	843:849	arg1	one					809:811	one	809:811	one	809:811	Meanwhile, as one of the PM2.5 common minerals, calcite fine particles were taken as the research object to explore the influence of microbial cells and extracellular polymers on its surface properties.
29247412	9	75	theme	surface	1858:1864	arg1	morphology					1836:1845	The morphology	1832:1845	The morphology of calcite surface	1832:1864	The morphology of calcite surface was significantly changed after dissolution and re-crystallization.
29247412	5	76	theme	electron	1216:1223	arg1	microscopy					1225:1234	scanning electron microscopy	1207:1234	scanning electron microscopy (SEM)	1207:1240	High performance liquid chromatography (HPLC), inductively coupled plasma spectrometry (ICP), Zeta potential analysis, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), and scanning electron microscopy (SEM) were used to characterize the composition of EPS, the soluble ions, surface charge, surface groups, crystal form, and surface morphology of calcite residual solid after being treated by the bacterial cells and EPS.
29247412	5	76	theme	electron	1216:1223	arg1	SEM					1237:1239	SEM	1237:1239	SEM	1237:1239	High performance liquid chromatography (HPLC), inductively coupled plasma spectrometry (ICP), Zeta potential analysis, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), and scanning electron microscopy (SEM) were used to characterize the composition of EPS, the soluble ions, surface charge, surface groups, crystal form, and surface morphology of calcite residual solid after being treated by the bacterial cells and EPS.
29247412	3	77	theme	extracellular	739:751	arg1	polymers					753:760	abundant extracellular polymers	730:760	abundant extracellular polymers	730:760	To explore the process and the results of microbial cells and their extracellular polymeric substances (EPS) acting on the surface properties of minerals of PM2.5 through the metabolism, a common native soil bacterium Bacillus mucitaginosus with abundant extracellular polymers was chosen as the tested strain.
29247412	5	78	theme	performance	1003:1013	arg1	HPLC					1038:1041	HPLC	1038:1041	HPLC	1038:1041	High performance liquid chromatography (HPLC), inductively coupled plasma spectrometry (ICP), Zeta potential analysis, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), and scanning electron microscopy (SEM) were used to characterize the composition of EPS, the soluble ions, surface charge, surface groups, crystal form, and surface morphology of calcite residual solid after being treated by the bacterial cells and EPS.
29247412	5	78	theme	performance	1003:1013	arg1	chromatography					1022:1035	High performance liquid chromatography	998:1035	High performance liquid chromatography (HPLC)	998:1042	High performance liquid chromatography (HPLC), inductively coupled plasma spectrometry (ICP), Zeta potential analysis, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), and scanning electron microscopy (SEM) were used to characterize the composition of EPS, the soluble ions, surface charge, surface groups, crystal form, and surface morphology of calcite residual solid after being treated by the bacterial cells and EPS.
29247412	10	79	theme	particulate	2134:2144	arg1	matter					2146:2151	PM fine particulate matter	2126:2151	PM fine particulate matter on human health impact	2126:2174	Experimental results also showed that the existence of the bacteria cells and EPS significantly affected the surface properties of calcite and provide a theoretical basis for the mechanism of PM fine particulate matter on human health impact for further study.
29247412	3	80	theme	tested	780:785	arg1	results					515:521	the results	511:521	the results of microbial cells and their extracellular polymeric substances (EPS) acting on the surface properties of minerals of PM2.5 through the metabolism, a common native soil bacterium Bacillus mucitaginosus with abundant extracellular polymers	511:760	To explore the process and the results of microbial cells and their extracellular polymeric substances (EPS) acting on the surface properties of minerals of PM2.5 through the metabolism, a common native soil bacterium Bacillus mucitaginosus with abundant extracellular polymers was chosen as the tested strain.
29247412	3	80	theme	tested	780:785	arg1	strain					787:792	the tested strain	776:792	the tested strain	776:792	To explore the process and the results of microbial cells and their extracellular polymeric substances (EPS) acting on the surface properties of minerals of PM2.5 through the metabolism, a common native soil bacterium Bacillus mucitaginosus with abundant extracellular polymers was chosen as the tested strain.
29247412	3	81	dep	Bacillus	702:709	arg1	mucitaginosus					711:723	mucitaginosus	711:723	mucitaginosus	711:723	To explore the process and the results of microbial cells and their extracellular polymeric substances (EPS) acting on the surface properties of minerals of PM2.5 through the metabolism, a common native soil bacterium Bacillus mucitaginosus with abundant extracellular polymers was chosen as the tested strain.
29247412	8	82	theme	organic	1680:1686	arg1	groups					1688:1693	organic groups	1680:1693	organic groups	1680:1693	Due to the adhesion of organic groups on the calcite surface, the surface potential shifted significantly in the negative direction and the solution pH was clearly increased.
29247412	7	83	theme	calcite	1620:1626	arg1	particles					1628:1636	calcite particles	1620:1636	calcite particles	1620:1636	Both the whole cell and its EPS could promote the dissolution of calcite particles into calcium ions.
29247412	4	84	theme	microbial	928:936	arg1	cells					938:942	microbial cells	928:942	microbial cells	928:942	Meanwhile, as one of the PM2.5 common minerals, calcite fine particles were taken as the research object to explore the influence of microbial cells and extracellular polymers on its surface properties.
29247412	5	85	theme	solid	1399:1403	arg1	composition					1272:1282	the composition	1268:1282	the composition of EPS, the soluble ions, surface charge, surface groups, crystal form, and surface morphology of calcite residual solid	1268:1403	High performance liquid chromatography (HPLC), inductively coupled plasma spectrometry (ICP), Zeta potential analysis, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), and scanning electron microscopy (SEM) were used to characterize the composition of EPS, the soluble ions, surface charge, surface groups, crystal form, and surface morphology of calcite residual solid after being treated by the bacterial cells and EPS.
29247412	10	86	theme	matter	2146:2151	arg1	mechanism					2113:2121	the mechanism	2109:2121	the mechanism of PM fine particulate matter on human health impact	2109:2174	Experimental results also showed that the existence of the bacteria cells and EPS significantly affected the surface properties of calcite and provide a theoretical basis for the mechanism of PM fine particulate matter on human health impact for further study.
29247412	0	87	theme	size	84:87	arg1	cells					99:103	same size bacterial cells	79:103	same size bacterial cells	79:103	Surface properties of PM2.5 calcite fine particulate matter in the presence of same size bacterial cells and exocellular polymeric substances (EPS) of Bacillus mucitaginosus.
29247412	0	88	from	matter	53:58	arg1	presence					67:74	the presence	63:74	the presence of same size bacterial cells and exocellular polymeric substances (EPS) of Bacillus mucitaginosus	63:172	Surface properties of PM2.5 calcite fine particulate matter in the presence of same size bacterial cells and exocellular polymeric substances (EPS) of Bacillus mucitaginosus.
29247412	10	89	theme	fine	2129:2132	arg1	matter					2146:2151	PM fine particulate matter	2126:2151	PM fine particulate matter on human health impact	2126:2174	Experimental results also showed that the existence of the bacteria cells and EPS significantly affected the surface properties of calcite and provide a theoretical basis for the mechanism of PM fine particulate matter on human health impact for further study.
29247412	0	90	theme	cells	99:103	arg1	presence					67:74	the presence	63:74	the presence of same size bacterial cells and exocellular polymeric substances (EPS) of Bacillus mucitaginosus	63:172	Surface properties of PM2.5 calcite fine particulate matter in the presence of same size bacterial cells and exocellular polymeric substances (EPS) of Bacillus mucitaginosus.
29247412	5	91	theme	coupled	1057:1063	arg1	ICP					1086:1088	ICP	1086:1088	ICP	1086:1088	High performance liquid chromatography (HPLC), inductively coupled plasma spectrometry (ICP), Zeta potential analysis, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), and scanning electron microscopy (SEM) were used to characterize the composition of EPS, the soluble ions, surface charge, surface groups, crystal form, and surface morphology of calcite residual solid after being treated by the bacterial cells and EPS.
29247412	5	91	theme	coupled	1057:1063	arg1	spectrometry					1072:1083	inductively coupled plasma spectrometry	1045:1083	inductively coupled plasma spectrometry (ICP)	1045:1089	High performance liquid chromatography (HPLC), inductively coupled plasma spectrometry (ICP), Zeta potential analysis, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), and scanning electron microscopy (SEM) were used to characterize the composition of EPS, the soluble ions, surface charge, surface groups, crystal form, and surface morphology of calcite residual solid after being treated by the bacterial cells and EPS.
29247412	8	92	theme	calcite	1702:1708	arg1	surface					1710:1716	the calcite surface	1698:1716	the calcite surface	1698:1716	Due to the adhesion of organic groups on the calcite surface, the surface potential shifted significantly in the negative direction and the solution pH was clearly increased.
29247412	5	93	theme	morphology	1368:1377	arg1	composition					1272:1282	the composition	1268:1282	the composition of EPS, the soluble ions, surface charge, surface groups, crystal form, and surface morphology of calcite residual solid	1268:1403	High performance liquid chromatography (HPLC), inductively coupled plasma spectrometry (ICP), Zeta potential analysis, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), and scanning electron microscopy (SEM) were used to characterize the composition of EPS, the soluble ions, surface charge, surface groups, crystal form, and surface morphology of calcite residual solid after being treated by the bacterial cells and EPS.
29247412	10	94	theme	human	2156:2160	arg1	impact					2169:2174	human health impact	2156:2174	human health impact	2156:2174	Experimental results also showed that the existence of the bacteria cells and EPS significantly affected the surface properties of calcite and provide a theoretical basis for the mechanism of PM fine particulate matter on human health impact for further study.
29247412	0	95	theme	PM2.5	22:26	arg1	properties					8:17	Surface properties	0:17	Surface properties of PM2.5	0:26	Surface properties of PM2.5 calcite fine particulate matter in the presence of same size bacterial cells and exocellular polymeric substances (EPS) of Bacillus mucitaginosus.
29247412	5	96	theme	infrared	1135:1142	arg1	FTIR					1158:1161	FTIR	1158:1161	FTIR	1158:1161	High performance liquid chromatography (HPLC), inductively coupled plasma spectrometry (ICP), Zeta potential analysis, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), and scanning electron microscopy (SEM) were used to characterize the composition of EPS, the soluble ions, surface charge, surface groups, crystal form, and surface morphology of calcite residual solid after being treated by the bacterial cells and EPS.
29247412	5	96	theme	infrared	1135:1142	arg1	spectroscopy					1144:1155	Fourier-transform infrared spectroscopy	1117:1155	Fourier-transform infrared spectroscopy (FTIR)	1117:1162	High performance liquid chromatography (HPLC), inductively coupled plasma spectrometry (ICP), Zeta potential analysis, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), and scanning electron microscopy (SEM) were used to characterize the composition of EPS, the soluble ions, surface charge, surface groups, crystal form, and surface morphology of calcite residual solid after being treated by the bacterial cells and EPS.
29247412	8	97	theme	surface	1723:1729	arg1	potential					1731:1739	the surface potential	1719:1739	the surface potential shifted significantly in the negative direction	1719:1787	Due to the adhesion of organic groups on the calcite surface, the surface potential shifted significantly in the negative direction and the solution pH was clearly increased.
29247412	3	98	theme	surface	607:613	arg1	properties					615:624	the surface properties	603:624	the surface properties of minerals of PM2.5 through the metabolism, a common native soil bacterium Bacillus mucitaginosus with abundant extracellular polymers	603:760	To explore the process and the results of microbial cells and their extracellular polymeric substances (EPS) acting on the surface properties of minerals of PM2.5 through the metabolism, a common native soil bacterium Bacillus mucitaginosus with abundant extracellular polymers was chosen as the tested strain.
29247412	0	99	theme	fine	36:39	arg1	matter					53:58	fine particulate matter	36:58	fine particulate matter in the presence of same size bacterial cells and exocellular polymeric substances (EPS) of Bacillus mucitaginosus	36:172	Surface properties of PM2.5 calcite fine particulate matter in the presence of same size bacterial cells and exocellular polymeric substances (EPS) of Bacillus mucitaginosus.
29247412	7	100	theme	whole	1564:1568	arg1	cell					1570:1573	the whole cell	1560:1573	the whole cell	1560:1573	Both the whole cell and its EPS could promote the dissolution of calcite particles into calcium ions.
29247412	5	101	theme	ions	1304:1307	arg1	composition					1272:1282	the composition	1268:1282	the composition of EPS, the soluble ions, surface charge, surface groups, crystal form, and surface morphology of calcite residual solid	1268:1403	High performance liquid chromatography (HPLC), inductively coupled plasma spectrometry (ICP), Zeta potential analysis, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), and scanning electron microscopy (SEM) were used to characterize the composition of EPS, the soluble ions, surface charge, surface groups, crystal form, and surface morphology of calcite residual solid after being treated by the bacterial cells and EPS.
29247412	0	102	theme	bacterial	89:97	arg1	cells					99:103	same size bacterial cells	79:103	same size bacterial cells	79:103	Surface properties of PM2.5 calcite fine particulate matter in the presence of same size bacterial cells and exocellular polymeric substances (EPS) of Bacillus mucitaginosus.
29247412	1	103	theme	fine	276:279	arg1	particles					289:297	fine mineral particles	276:297	PM2.5 (fine particulate matter) as well as fine mineral particles	233:297	Microorganism cells and spores are the main components of PM2.5 (fine particulate matter) as well as fine mineral particles.
29247412	4	104	theme	polymers	962:969	arg1	influence					915:923	the influence	911:923	the influence of microbial cells and extracellular polymers on its surface properties	911:995	Meanwhile, as one of the PM2.5 common minerals, calcite fine particles were taken as the research object to explore the influence of microbial cells and extracellular polymers on its surface properties.
29247412	5	105	theme	surface	1310:1316	arg1	charge					1318:1323	surface charge	1310:1323	surface charge	1310:1323	High performance liquid chromatography (HPLC), inductively coupled plasma spectrometry (ICP), Zeta potential analysis, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), and scanning electron microscopy (SEM) were used to characterize the composition of EPS, the soluble ions, surface charge, surface groups, crystal form, and surface morphology of calcite residual solid after being treated by the bacterial cells and EPS.
29247412	1	106	theme	particles	289:297	arg1	components					219:228	the main components	210:228	the main components of PM2.5 (fine particulate matter) as well as fine mineral particles	210:297	Microorganism cells and spores are the main components of PM2.5 (fine particulate matter) as well as fine mineral particles.
29247412	1	106	theme	particles	289:297	arg1	spores					199:204	spores	199:204	spores	199:204	Microorganism cells and spores are the main components of PM2.5 (fine particulate matter) as well as fine mineral particles.
29247412	1	106	theme	particles	289:297	arg1	cells					189:193	Microorganism cells	175:193	Microorganism cells	175:193	Microorganism cells and spores are the main components of PM2.5 (fine particulate matter) as well as fine mineral particles.
29247412	5	107	theme	bacterial	1432:1440	arg1	cells					1442:1446	the bacterial cells	1428:1446	the bacterial cells	1428:1446	High performance liquid chromatography (HPLC), inductively coupled plasma spectrometry (ICP), Zeta potential analysis, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), and scanning electron microscopy (SEM) were used to characterize the composition of EPS, the soluble ions, surface charge, surface groups, crystal form, and surface morphology of calcite residual solid after being treated by the bacterial cells and EPS.
29247412	5	108	theme	EPS	1287:1289	arg1	composition					1272:1282	the composition	1268:1282	the composition of EPS, the soluble ions, surface charge, surface groups, crystal form, and surface morphology of calcite residual solid	1268:1403	High performance liquid chromatography (HPLC), inductively coupled plasma spectrometry (ICP), Zeta potential analysis, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), and scanning electron microscopy (SEM) were used to characterize the composition of EPS, the soluble ions, surface charge, surface groups, crystal form, and surface morphology of calcite residual solid after being treated by the bacterial cells and EPS.
29247412	1	109	theme	main	214:217	arg1	components					219:228	the main components	210:228	the main components of PM2.5 (fine particulate matter) as well as fine mineral particles	210:297	Microorganism cells and spores are the main components of PM2.5 (fine particulate matter) as well as fine mineral particles.
29247412	1	109	theme	main	214:217	arg1	spores					199:204	spores	199:204	spores	199:204	Microorganism cells and spores are the main components of PM2.5 (fine particulate matter) as well as fine mineral particles.
29247412	1	109	theme	main	214:217	arg1	cells					189:193	Microorganism cells	175:193	Microorganism cells	175:193	Microorganism cells and spores are the main components of PM2.5 (fine particulate matter) as well as fine mineral particles.
29247412	10	110	from	mechanism	2113:2121	arg1	impact					2169:2174	human health impact	2156:2174	human health impact	2156:2174	Experimental results also showed that the existence of the bacteria cells and EPS significantly affected the surface properties of calcite and provide a theoretical basis for the mechanism of PM fine particulate matter on human health impact for further study.
29247412	4	111	from	influence	915:923	arg1	properties					986:995	its surface properties	974:995	its surface properties	974:995	Meanwhile, as one of the PM2.5 common minerals, calcite fine particles were taken as the research object to explore the influence of microbial cells and extracellular polymers on its surface properties.
25820340	6	0	theme	optimum	1486:1492	arg1	value					1494:1498	the optimum value	1482:1498	the optimum value	1482:1498	The simultaneous analysis in 20 min total runtime of the 15 carbohydrates plus internal reference (naphthalene sulfonate) was carried out at 25°C with a BGE composed of 77.4 mM NaOH and 183 mM NaCl to adjust the conductivity at the optimum value.
25820340	1	1	theme	CE	177:178	arg1	method					180:185	a CE method	175:185	a CE method allowing, for the first time, the simultaneous separation of the underivatized first seven cellodextrin oligomers (glucose, cellobiose, cellotriose, cellotetraose, cellopentaose, cellohexaose, and celloheptaose), with a view to analyze the hydrolysates obtained after partial acid depolymerization of nitrocellulose, and eight carbohydrates (ribose, xylose, fructose, mannose, galactose, maltose, lactose, and sucrose), which might be potential interfering compounds in explosives samples	175:674	This work focuses on the development of a CE method allowing, for the first time, the simultaneous separation of the underivatized first seven cellodextrin oligomers (glucose, cellobiose, cellotriose, cellotetraose, cellopentaose, cellohexaose, and celloheptaose), with a view to analyze the hydrolysates obtained after partial acid depolymerization of nitrocellulose, and eight carbohydrates (ribose, xylose, fructose, mannose, galactose, maltose, lactose, and sucrose), which might be potential interfering compounds in explosives samples.
25820340	2	2	theme	detection	837:845	arg1	window					847:852	the detection window	833:852	the detection window	833:852	Separation was achieved with a highly alkaline BGE containing sodium chloride and direct mid-UV-absorbance detection was performed after photo-oxidation in the detection window.
25820340	1	3	theme	underivatized	252:264	arg1	oligomers					291:299	the underivatized first seven cellodextrin oligomers	248:299	the underivatized first seven cellodextrin oligomers (glucose, cellobiose, cellotriose, cellotetraose, cellopentaose, cellohexaose, and celloheptaose)	248:397	This work focuses on the development of a CE method allowing, for the first time, the simultaneous separation of the underivatized first seven cellodextrin oligomers (glucose, cellobiose, cellotriose, cellotetraose, cellopentaose, cellohexaose, and celloheptaose), with a view to analyze the hydrolysates obtained after partial acid depolymerization of nitrocellulose, and eight carbohydrates (ribose, xylose, fructose, mannose, galactose, maltose, lactose, and sucrose), which might be potential interfering compounds in explosives samples.
25820340	1	4	from	compounds	644:652	arg1	samples					668:674	explosives samples	657:674	explosives samples	657:674	This work focuses on the development of a CE method allowing, for the first time, the simultaneous separation of the underivatized first seven cellodextrin oligomers (glucose, cellobiose, cellotriose, cellotetraose, cellopentaose, cellohexaose, and celloheptaose), with a view to analyze the hydrolysates obtained after partial acid depolymerization of nitrocellulose, and eight carbohydrates (ribose, xylose, fructose, mannose, galactose, maltose, lactose, and sucrose), which might be potential interfering compounds in explosives samples.
25820340	1	5	theme	method	180:185	arg1	development					160:170	the development	156:170	the development of a CE method allowing, for the first time, the simultaneous separation of the underivatized first seven cellodextrin oligomers (glucose, cellobiose, cellotriose, cellotetraose, cellopentaose, cellohexaose, and celloheptaose), with a view to analyze the hydrolysates obtained after partial acid depolymerization of nitrocellulose, and eight carbohydrates (ribose, xylose, fructose, mannose, galactose, maltose, lactose, and sucrose), which might be potential interfering compounds in explosives samples	156:674	This work focuses on the development of a CE method allowing, for the first time, the simultaneous separation of the underivatized first seven cellodextrin oligomers (glucose, cellobiose, cellotriose, cellotetraose, cellopentaose, cellohexaose, and celloheptaose), with a view to analyze the hydrolysates obtained after partial acid depolymerization of nitrocellulose, and eight carbohydrates (ribose, xylose, fructose, mannose, galactose, maltose, lactose, and sucrose), which might be potential interfering compounds in explosives samples.
25820340	4	6	theme	concentration	1067:1079	arg1	effects					1018:1024	the effects	1014:1024	the effects of BGE conductivity and sodium hydroxide concentration on resolutions between neighboring peaks, and analysis time	1014:1139	A central composite design was carried out to determine the effects of BGE conductivity and sodium hydroxide concentration on resolutions between neighboring peaks, and analysis time.
25820340	1	7	theme	first	266:270	arg1	oligomers					291:299	the underivatized first seven cellodextrin oligomers	248:299	the underivatized first seven cellodextrin oligomers (glucose, cellobiose, cellotriose, cellotetraose, cellopentaose, cellohexaose, and celloheptaose)	248:397	This work focuses on the development of a CE method allowing, for the first time, the simultaneous separation of the underivatized first seven cellodextrin oligomers (glucose, cellobiose, cellotriose, cellotetraose, cellopentaose, cellohexaose, and celloheptaose), with a view to analyze the hydrolysates obtained after partial acid depolymerization of nitrocellulose, and eight carbohydrates (ribose, xylose, fructose, mannose, galactose, maltose, lactose, and sucrose), which might be potential interfering compounds in explosives samples.
25820340	5	8	theme	desirability	1144:1155	arg1	analysis					1157:1164	A desirability analysis	1142:1164	A desirability analysis on modeled responses	1142:1185	A desirability analysis on modeled responses was applied to maximize resolutions and to minimize analysis time.
25820340	5	9	from	analysis	1157:1164	arg1	responses					1177:1185	modeled responses	1169:1185	modeled responses	1169:1185	A desirability analysis on modeled responses was applied to maximize resolutions and to minimize analysis time.
25820340	6	10	from	analysis	1271:1278	arg1	reference					1342:1350	internal reference	1333:1350	internal reference (naphthalene sulfonate)	1333:1374	The simultaneous analysis in 20 min total runtime of the 15 carbohydrates plus internal reference (naphthalene sulfonate) was carried out at 25°C with a BGE composed of 77.4 mM NaOH and 183 mM NaCl to adjust the conductivity at the optimum value.
25820340	6	10	from	analysis	1271:1278	arg1	sulfonate					1365:1373	naphthalene sulfonate	1353:1373	naphthalene sulfonate	1353:1373	The simultaneous analysis in 20 min total runtime of the 15 carbohydrates plus internal reference (naphthalene sulfonate) was carried out at 25°C with a BGE composed of 77.4 mM NaOH and 183 mM NaCl to adjust the conductivity at the optimum value.
25820340	6	10	from	analysis	1271:1278	arg1	runtime					1296:1302	20 min total runtime	1283:1302	20 min total runtime of the 15 carbohydrates	1283:1326	The simultaneous analysis in 20 min total runtime of the 15 carbohydrates plus internal reference (naphthalene sulfonate) was carried out at 25°C with a BGE composed of 77.4 mM NaOH and 183 mM NaCl to adjust the conductivity at the optimum value.
25820340	4	11	theme	hydroxide	1057:1065	arg1	concentration					1067:1079	sodium hydroxide concentration	1050:1079	sodium hydroxide concentration	1050:1079	A central composite design was carried out to determine the effects of BGE conductivity and sodium hydroxide concentration on resolutions between neighboring peaks, and analysis time.
25820340	2	12	theme	alkaline	715:722	arg1	BGE					724:726	a highly alkaline BGE	706:726	a highly alkaline BGE containing sodium chloride	706:753	Separation was achieved with a highly alkaline BGE containing sodium chloride and direct mid-UV-absorbance detection was performed after photo-oxidation in the detection window.
25820340	6	13	theme	min	1286:1288	arg1	runtime					1296:1302	20 min total runtime	1283:1302	20 min total runtime of the 15 carbohydrates	1283:1326	The simultaneous analysis in 20 min total runtime of the 15 carbohydrates plus internal reference (naphthalene sulfonate) was carried out at 25°C with a BGE composed of 77.4 mM NaOH and 183 mM NaCl to adjust the conductivity at the optimum value.
25820340	3	14	theme	capillary	913:921	arg1	coating					923:929	a dynamic capillary coating	903:929	a dynamic capillary coating by hexadimethrine bromide	903:955	EOF was reversed to speed up the analysis using a dynamic capillary coating by hexadimethrine bromide.
25820340	1	15	theme	cellodextrin	278:289	arg1	oligomers					291:299	the underivatized first seven cellodextrin oligomers	248:299	the underivatized first seven cellodextrin oligomers (glucose, cellobiose, cellotriose, cellotetraose, cellopentaose, cellohexaose, and celloheptaose)	248:397	This work focuses on the development of a CE method allowing, for the first time, the simultaneous separation of the underivatized first seven cellodextrin oligomers (glucose, cellobiose, cellotriose, cellotetraose, cellopentaose, cellohexaose, and celloheptaose), with a view to analyze the hydrolysates obtained after partial acid depolymerization of nitrocellulose, and eight carbohydrates (ribose, xylose, fructose, mannose, galactose, maltose, lactose, and sucrose), which might be potential interfering compounds in explosives samples.
25820340	4	16	theme	central	960:966	arg1	design					978:983	A central composite design	958:983	A central composite design	958:983	A central composite design was carried out to determine the effects of BGE conductivity and sodium hydroxide concentration on resolutions between neighboring peaks, and analysis time.
25820340	6	17	theme	20	1283:1284	arg1	min					1286:1288	min	1286:1288	min	1286:1288	The simultaneous analysis in 20 min total runtime of the 15 carbohydrates plus internal reference (naphthalene sulfonate) was carried out at 25°C with a BGE composed of 77.4 mM NaOH and 183 mM NaCl to adjust the conductivity at the optimum value.
25820340	1	18	theme	partial	455:461	arg1	depolymerization					468:483	partial acid depolymerization	455:483	partial acid depolymerization	455:483	This work focuses on the development of a CE method allowing, for the first time, the simultaneous separation of the underivatized first seven cellodextrin oligomers (glucose, cellobiose, cellotriose, cellotetraose, cellopentaose, cellohexaose, and celloheptaose), with a view to analyze the hydrolysates obtained after partial acid depolymerization of nitrocellulose, and eight carbohydrates (ribose, xylose, fructose, mannose, galactose, maltose, lactose, and sucrose), which might be potential interfering compounds in explosives samples.
25820340	1	19	dep	carbohydrates	514:526	arg1	ribose					529:534	ribose	529:534	ribose	529:534	This work focuses on the development of a CE method allowing, for the first time, the simultaneous separation of the underivatized first seven cellodextrin oligomers (glucose, cellobiose, cellotriose, cellotetraose, cellopentaose, cellohexaose, and celloheptaose), with a view to analyze the hydrolysates obtained after partial acid depolymerization of nitrocellulose, and eight carbohydrates (ribose, xylose, fructose, mannose, galactose, maltose, lactose, and sucrose), which might be potential interfering compounds in explosives samples.
25820340	1	19	dep	carbohydrates	514:526	arg1	xylose					537:542	xylose	537:542	xylose	537:542	This work focuses on the development of a CE method allowing, for the first time, the simultaneous separation of the underivatized first seven cellodextrin oligomers (glucose, cellobiose, cellotriose, cellotetraose, cellopentaose, cellohexaose, and celloheptaose), with a view to analyze the hydrolysates obtained after partial acid depolymerization of nitrocellulose, and eight carbohydrates (ribose, xylose, fructose, mannose, galactose, maltose, lactose, and sucrose), which might be potential interfering compounds in explosives samples.
25820340	1	19	dep	carbohydrates	514:526	arg1	galactose					564:572	galactose	564:572	galactose	564:572	This work focuses on the development of a CE method allowing, for the first time, the simultaneous separation of the underivatized first seven cellodextrin oligomers (glucose, cellobiose, cellotriose, cellotetraose, cellopentaose, cellohexaose, and celloheptaose), with a view to analyze the hydrolysates obtained after partial acid depolymerization of nitrocellulose, and eight carbohydrates (ribose, xylose, fructose, mannose, galactose, maltose, lactose, and sucrose), which might be potential interfering compounds in explosives samples.
25820340	1	19	dep	carbohydrates	514:526	arg1	sucrose					597:603	sucrose	597:603	sucrose	597:603	This work focuses on the development of a CE method allowing, for the first time, the simultaneous separation of the underivatized first seven cellodextrin oligomers (glucose, cellobiose, cellotriose, cellotetraose, cellopentaose, cellohexaose, and celloheptaose), with a view to analyze the hydrolysates obtained after partial acid depolymerization of nitrocellulose, and eight carbohydrates (ribose, xylose, fructose, mannose, galactose, maltose, lactose, and sucrose), which might be potential interfering compounds in explosives samples.
25820340	1	19	dep	carbohydrates	514:526	arg1	maltose					575:581	maltose	575:581	maltose	575:581	This work focuses on the development of a CE method allowing, for the first time, the simultaneous separation of the underivatized first seven cellodextrin oligomers (glucose, cellobiose, cellotriose, cellotetraose, cellopentaose, cellohexaose, and celloheptaose), with a view to analyze the hydrolysates obtained after partial acid depolymerization of nitrocellulose, and eight carbohydrates (ribose, xylose, fructose, mannose, galactose, maltose, lactose, and sucrose), which might be potential interfering compounds in explosives samples.
25820340	1	19	dep	carbohydrates	514:526	arg1	carbohydrates					514:526	eight carbohydrates	508:526	eight carbohydrates (ribose, xylose, fructose, mannose, galactose, maltose, lactose, and sucrose)	508:604	This work focuses on the development of a CE method allowing, for the first time, the simultaneous separation of the underivatized first seven cellodextrin oligomers (glucose, cellobiose, cellotriose, cellotetraose, cellopentaose, cellohexaose, and celloheptaose), with a view to analyze the hydrolysates obtained after partial acid depolymerization of nitrocellulose, and eight carbohydrates (ribose, xylose, fructose, mannose, galactose, maltose, lactose, and sucrose), which might be potential interfering compounds in explosives samples.
25820340	1	19	dep	carbohydrates	514:526	arg1	fructose					545:552	fructose	545:552	fructose	545:552	This work focuses on the development of a CE method allowing, for the first time, the simultaneous separation of the underivatized first seven cellodextrin oligomers (glucose, cellobiose, cellotriose, cellotetraose, cellopentaose, cellohexaose, and celloheptaose), with a view to analyze the hydrolysates obtained after partial acid depolymerization of nitrocellulose, and eight carbohydrates (ribose, xylose, fructose, mannose, galactose, maltose, lactose, and sucrose), which might be potential interfering compounds in explosives samples.
25820340	1	19	dep	carbohydrates	514:526	arg1	mannose					555:561	mannose	555:561	mannose	555:561	This work focuses on the development of a CE method allowing, for the first time, the simultaneous separation of the underivatized first seven cellodextrin oligomers (glucose, cellobiose, cellotriose, cellotetraose, cellopentaose, cellohexaose, and celloheptaose), with a view to analyze the hydrolysates obtained after partial acid depolymerization of nitrocellulose, and eight carbohydrates (ribose, xylose, fructose, mannose, galactose, maltose, lactose, and sucrose), which might be potential interfering compounds in explosives samples.
25820340	1	19	dep	carbohydrates	514:526	arg1	lactose					584:590	lactose	584:590	lactose	584:590	This work focuses on the development of a CE method allowing, for the first time, the simultaneous separation of the underivatized first seven cellodextrin oligomers (glucose, cellobiose, cellotriose, cellotetraose, cellopentaose, cellohexaose, and celloheptaose), with a view to analyze the hydrolysates obtained after partial acid depolymerization of nitrocellulose, and eight carbohydrates (ribose, xylose, fructose, mannose, galactose, maltose, lactose, and sucrose), which might be potential interfering compounds in explosives samples.
25820340	1	20	dep	analyze	415:421	arg1	obtained					440:447	obtained	440:447	obtained after partial acid depolymerization of nitrocellulose, and eight carbohydrates (ribose, xylose, fructose, mannose, galactose, maltose, lactose, and sucrose), which might be potential interfering compounds in explosives samples	440:674	This work focuses on the development of a CE method allowing, for the first time, the simultaneous separation of the underivatized first seven cellodextrin oligomers (glucose, cellobiose, cellotriose, cellotetraose, cellopentaose, cellohexaose, and celloheptaose), with a view to analyze the hydrolysates obtained after partial acid depolymerization of nitrocellulose, and eight carbohydrates (ribose, xylose, fructose, mannose, galactose, maltose, lactose, and sucrose), which might be potential interfering compounds in explosives samples.
25820340	1	21	theme	acid	463:466	arg1	depolymerization					468:483	partial acid depolymerization	455:483	partial acid depolymerization	455:483	This work focuses on the development of a CE method allowing, for the first time, the simultaneous separation of the underivatized first seven cellodextrin oligomers (glucose, cellobiose, cellotriose, cellotetraose, cellopentaose, cellohexaose, and celloheptaose), with a view to analyze the hydrolysates obtained after partial acid depolymerization of nitrocellulose, and eight carbohydrates (ribose, xylose, fructose, mannose, galactose, maltose, lactose, and sucrose), which might be potential interfering compounds in explosives samples.
25820340	4	22	from	effects	1018:1024	arg1	resolutions					1084:1094	resolutions	1084:1094	resolutions between neighboring peaks, and analysis time	1084:1139	A central composite design was carried out to determine the effects of BGE conductivity and sodium hydroxide concentration on resolutions between neighboring peaks, and analysis time.
25820340	1	23	dep	oligomers	291:299	arg1	glucose					302:308	glucose	302:308	glucose	302:308	This work focuses on the development of a CE method allowing, for the first time, the simultaneous separation of the underivatized first seven cellodextrin oligomers (glucose, cellobiose, cellotriose, cellotetraose, cellopentaose, cellohexaose, and celloheptaose), with a view to analyze the hydrolysates obtained after partial acid depolymerization of nitrocellulose, and eight carbohydrates (ribose, xylose, fructose, mannose, galactose, maltose, lactose, and sucrose), which might be potential interfering compounds in explosives samples.
25820340	1	24	theme	oligomers	291:299	arg1	separation					234:243	the simultaneous separation	217:243	the simultaneous separation of the underivatized first seven cellodextrin oligomers (glucose, cellobiose, cellotriose, cellotetraose, cellopentaose, cellohexaose, and celloheptaose), with a view to analyze the hydrolysates obtained after partial acid depolymerization of nitrocellulose, and eight carbohydrates (ribose, xylose, fructose, mannose, galactose, maltose, lactose, and sucrose), which might be potential interfering compounds in explosives samples	217:674	This work focuses on the development of a CE method allowing, for the first time, the simultaneous separation of the underivatized first seven cellodextrin oligomers (glucose, cellobiose, cellotriose, cellotetraose, cellopentaose, cellohexaose, and celloheptaose), with a view to analyze the hydrolysates obtained after partial acid depolymerization of nitrocellulose, and eight carbohydrates (ribose, xylose, fructose, mannose, galactose, maltose, lactose, and sucrose), which might be potential interfering compounds in explosives samples.
25820340	6	25	theme	simultaneous	1258:1269	arg1	analysis					1271:1278	The simultaneous analysis	1254:1278	The simultaneous analysis in 20 min total runtime of the 15 carbohydrates plus internal reference (naphthalene sulfonate)	1254:1374	The simultaneous analysis in 20 min total runtime of the 15 carbohydrates plus internal reference (naphthalene sulfonate) was carried out at 25°C with a BGE composed of 77.4 mM NaOH and 183 mM NaCl to adjust the conductivity at the optimum value.
25820340	1	26	with	oligomers	291:299	arg1	view					407:410	a view to analyze the hydrolysates obtained after partial acid depolymerization of nitrocellulose, and eight carbohydrates (ribose, xylose, fructose, mannose, galactose, maltose, lactose, and sucrose), which might be potential interfering compounds in explosives samples	405:674	a view to analyze the hydrolysates obtained after partial acid depolymerization of nitrocellulose, and eight carbohydrates (ribose, xylose, fructose, mannose, galactose, maltose, lactose, and sucrose), which might be potential interfering compounds in explosives samples	405:674	This work focuses on the development of a CE method allowing, for the first time, the simultaneous separation of the underivatized first seven cellodextrin oligomers (glucose, cellobiose, cellotriose, cellotetraose, cellopentaose, cellohexaose, and celloheptaose), with a view to analyze the hydrolysates obtained after partial acid depolymerization of nitrocellulose, and eight carbohydrates (ribose, xylose, fructose, mannose, galactose, maltose, lactose, and sucrose), which might be potential interfering compounds in explosives samples.
25820340	7	27	theme	resolution	1514:1523	arg1	robustness					1525:1534	the resolution robustness	1510:1534	the resolution robustness	1510:1534	Finally, the resolution robustness was checked.
25820340	1	28	theme	potential	622:630	arg1	compounds					644:652	potential interfering compounds	622:652	potential interfering compounds	622:652	This work focuses on the development of a CE method allowing, for the first time, the simultaneous separation of the underivatized first seven cellodextrin oligomers (glucose, cellobiose, cellotriose, cellotetraose, cellopentaose, cellohexaose, and celloheptaose), with a view to analyze the hydrolysates obtained after partial acid depolymerization of nitrocellulose, and eight carbohydrates (ribose, xylose, fructose, mannose, galactose, maltose, lactose, and sucrose), which might be potential interfering compounds in explosives samples.
25820340	6	29	theme	naphthalene	1353:1363	arg1	reference					1342:1350	internal reference	1333:1350	internal reference (naphthalene sulfonate)	1333:1374	The simultaneous analysis in 20 min total runtime of the 15 carbohydrates plus internal reference (naphthalene sulfonate) was carried out at 25°C with a BGE composed of 77.4 mM NaOH and 183 mM NaCl to adjust the conductivity at the optimum value.
25820340	6	29	theme	naphthalene	1353:1363	arg1	sulfonate					1365:1373	naphthalene sulfonate	1353:1373	naphthalene sulfonate	1353:1373	The simultaneous analysis in 20 min total runtime of the 15 carbohydrates plus internal reference (naphthalene sulfonate) was carried out at 25°C with a BGE composed of 77.4 mM NaOH and 183 mM NaCl to adjust the conductivity at the optimum value.
25820340	1	30	theme	nitrocellulose	488:501	arg1	depolymerization					468:483	partial acid depolymerization	455:483	partial acid depolymerization	455:483	This work focuses on the development of a CE method allowing, for the first time, the simultaneous separation of the underivatized first seven cellodextrin oligomers (glucose, cellobiose, cellotriose, cellotetraose, cellopentaose, cellohexaose, and celloheptaose), with a view to analyze the hydrolysates obtained after partial acid depolymerization of nitrocellulose, and eight carbohydrates (ribose, xylose, fructose, mannose, galactose, maltose, lactose, and sucrose), which might be potential interfering compounds in explosives samples.
25820340	2	31	theme	mid-UV-absorbance	766:782	arg1	detection					784:792	direct mid-UV-absorbance detection	759:792	direct mid-UV-absorbance detection	759:792	Separation was achieved with a highly alkaline BGE containing sodium chloride and direct mid-UV-absorbance detection was performed after photo-oxidation in the detection window.
25820340	1	32	theme	first	205:209	arg1	time					211:214	the first time	201:214	the first time	201:214	This work focuses on the development of a CE method allowing, for the first time, the simultaneous separation of the underivatized first seven cellodextrin oligomers (glucose, cellobiose, cellotriose, cellotetraose, cellopentaose, cellohexaose, and celloheptaose), with a view to analyze the hydrolysates obtained after partial acid depolymerization of nitrocellulose, and eight carbohydrates (ribose, xylose, fructose, mannose, galactose, maltose, lactose, and sucrose), which might be potential interfering compounds in explosives samples.
25820340	1	33	theme	interfering	632:642	arg1	compounds					644:652	potential interfering compounds	622:652	potential interfering compounds	622:652	This work focuses on the development of a CE method allowing, for the first time, the simultaneous separation of the underivatized first seven cellodextrin oligomers (glucose, cellobiose, cellotriose, cellotetraose, cellopentaose, cellohexaose, and celloheptaose), with a view to analyze the hydrolysates obtained after partial acid depolymerization of nitrocellulose, and eight carbohydrates (ribose, xylose, fructose, mannose, galactose, maltose, lactose, and sucrose), which might be potential interfering compounds in explosives samples.
25820340	6	34	theme	mM	1428:1429	arg1	NaOH					1431:1434	77.4 mM NaOH	1423:1434	77.4 mM NaOH	1423:1434	The simultaneous analysis in 20 min total runtime of the 15 carbohydrates plus internal reference (naphthalene sulfonate) was carried out at 25°C with a BGE composed of 77.4 mM NaOH and 183 mM NaCl to adjust the conductivity at the optimum value.
25820340	2	35	contain	containing	728:737	arg1	BGE					724:726	a highly alkaline BGE	706:726	a highly alkaline BGE containing sodium chloride	706:753	Separation was achieved with a highly alkaline BGE containing sodium chloride and direct mid-UV-absorbance detection was performed after photo-oxidation in the detection window.
25820340	2	35	contain	containing	728:737	arg2	chloride					746:753	sodium chloride	739:753	sodium chloride	739:753	Separation was achieved with a highly alkaline BGE containing sodium chloride and direct mid-UV-absorbance detection was performed after photo-oxidation in the detection window.
25820340	2	36	theme	direct	759:764	arg1	detection					784:792	direct mid-UV-absorbance detection	759:792	direct mid-UV-absorbance detection	759:792	Separation was achieved with a highly alkaline BGE containing sodium chloride and direct mid-UV-absorbance detection was performed after photo-oxidation in the detection window.
25820340	2	37	theme	sodium	739:744	arg1	chloride					746:753	sodium chloride	739:753	sodium chloride	739:753	Separation was achieved with a highly alkaline BGE containing sodium chloride and direct mid-UV-absorbance detection was performed after photo-oxidation in the detection window.
25820340	6	38	theme	mM	1444:1445	arg1	NaCl					1447:1450	183 mM NaCl	1440:1450	183 mM NaCl	1440:1450	The simultaneous analysis in 20 min total runtime of the 15 carbohydrates plus internal reference (naphthalene sulfonate) was carried out at 25°C with a BGE composed of 77.4 mM NaOH and 183 mM NaCl to adjust the conductivity at the optimum value.
25820340	4	39	theme	conductivity	1033:1044	arg1	effects					1018:1024	the effects	1014:1024	the effects of BGE conductivity and sodium hydroxide concentration on resolutions between neighboring peaks, and analysis time	1014:1139	A central composite design was carried out to determine the effects of BGE conductivity and sodium hydroxide concentration on resolutions between neighboring peaks, and analysis time.
25820340	0	40	theme	oligosaccharides	39:54	arg1	Analysis					0:7	Analysis	0:7	Analysis of underivatized cellodextrin oligosaccharides by capillary electrophoresis with direct photochemically	0:111	Analysis of underivatized cellodextrin oligosaccharides by capillary electrophoresis with direct photochemically induced UV-detection.
25820340	8	41	theme	new	1554:1556	arg1	method					1558:1563	This new method	1549:1563	This new method	1549:1563	This new method should also be of interest to monitor food and nonfood crop products.
25820340	6	42	theme	internal	1333:1340	arg1	reference					1342:1350	internal reference	1333:1350	internal reference (naphthalene sulfonate)	1333:1374	The simultaneous analysis in 20 min total runtime of the 15 carbohydrates plus internal reference (naphthalene sulfonate) was carried out at 25°C with a BGE composed of 77.4 mM NaOH and 183 mM NaCl to adjust the conductivity at the optimum value.
25820340	6	42	theme	internal	1333:1340	arg1	sulfonate					1365:1373	naphthalene sulfonate	1353:1373	naphthalene sulfonate	1353:1373	The simultaneous analysis in 20 min total runtime of the 15 carbohydrates plus internal reference (naphthalene sulfonate) was carried out at 25°C with a BGE composed of 77.4 mM NaOH and 183 mM NaCl to adjust the conductivity at the optimum value.
25820340	0	43	theme	cellodextrin	26:37	arg1	oligosaccharides					39:54	underivatized cellodextrin oligosaccharides	12:54	underivatized cellodextrin oligosaccharides	12:54	Analysis of underivatized cellodextrin oligosaccharides by capillary electrophoresis with direct photochemically induced UV-detection.
25820340	1	44	theme	explosives	657:666	arg1	samples					668:674	explosives samples	657:674	explosives samples	657:674	This work focuses on the development of a CE method allowing, for the first time, the simultaneous separation of the underivatized first seven cellodextrin oligomers (glucose, cellobiose, cellotriose, cellotetraose, cellopentaose, cellohexaose, and celloheptaose), with a view to analyze the hydrolysates obtained after partial acid depolymerization of nitrocellulose, and eight carbohydrates (ribose, xylose, fructose, mannose, galactose, maltose, lactose, and sucrose), which might be potential interfering compounds in explosives samples.
25820340	3	45	theme	dynamic	905:911	arg1	coating					923:929	a dynamic capillary coating	903:929	a dynamic capillary coating by hexadimethrine bromide	903:955	EOF was reversed to speed up the analysis using a dynamic capillary coating by hexadimethrine bromide.
25820340	4	46	theme	analysis	1127:1134	arg1	time					1136:1139	analysis time	1127:1139	analysis time	1127:1139	A central composite design was carried out to determine the effects of BGE conductivity and sodium hydroxide concentration on resolutions between neighboring peaks, and analysis time.
25820340	0	47	theme	capillary	59:67	arg1	electrophoresis					69:83	capillary electrophoresis	59:83	capillary electrophoresis with direct photochemically	59:111	Analysis of underivatized cellodextrin oligosaccharides by capillary electrophoresis with direct photochemically induced UV-detection.
25820340	5	48	theme	modeled	1169:1175	arg1	responses					1177:1185	modeled responses	1169:1185	modeled responses	1169:1185	A desirability analysis on modeled responses was applied to maximize resolutions and to minimize analysis time.
25820340	6	49	theme	carbohydrates	1314:1326	arg1	runtime					1296:1302	20 min total runtime	1283:1302	20 min total runtime of the 15 carbohydrates	1283:1326	The simultaneous analysis in 20 min total runtime of the 15 carbohydrates plus internal reference (naphthalene sulfonate) was carried out at 25°C with a BGE composed of 77.4 mM NaOH and 183 mM NaCl to adjust the conductivity at the optimum value.
25820340	6	50	theme	total	1290:1294	arg1	runtime					1296:1302	20 min total runtime	1283:1302	20 min total runtime of the 15 carbohydrates	1283:1326	The simultaneous analysis in 20 min total runtime of the 15 carbohydrates plus internal reference (naphthalene sulfonate) was carried out at 25°C with a BGE composed of 77.4 mM NaOH and 183 mM NaCl to adjust the conductivity at the optimum value.
25820340	8	51	theme	crop	1620:1623	arg1	products					1625:1632	nonfood crop products	1612:1632	nonfood crop products	1612:1632	This new method should also be of interest to monitor food and nonfood crop products.
25820340	4	52	theme	BGE	1029:1031	arg1	conductivity					1033:1044	BGE conductivity	1029:1044	BGE conductivity	1029:1044	A central composite design was carried out to determine the effects of BGE conductivity and sodium hydroxide concentration on resolutions between neighboring peaks, and analysis time.
25820340	1	53	theme	carbohydrates	514:526	arg1	depolymerization					468:483	partial acid depolymerization	455:483	partial acid depolymerization	455:483	This work focuses on the development of a CE method allowing, for the first time, the simultaneous separation of the underivatized first seven cellodextrin oligomers (glucose, cellobiose, cellotriose, cellotetraose, cellopentaose, cellohexaose, and celloheptaose), with a view to analyze the hydrolysates obtained after partial acid depolymerization of nitrocellulose, and eight carbohydrates (ribose, xylose, fructose, mannose, galactose, maltose, lactose, and sucrose), which might be potential interfering compounds in explosives samples.
25820340	8	54	theme	nonfood	1612:1618	arg1	products					1625:1632	nonfood crop products	1612:1632	nonfood crop products	1612:1632	This new method should also be of interest to monitor food and nonfood crop products.
25820340	1	55	theme	simultaneous	221:232	arg1	separation					234:243	the simultaneous separation	217:243	the simultaneous separation of the underivatized first seven cellodextrin oligomers (glucose, cellobiose, cellotriose, cellotetraose, cellopentaose, cellohexaose, and celloheptaose), with a view to analyze the hydrolysates obtained after partial acid depolymerization of nitrocellulose, and eight carbohydrates (ribose, xylose, fructose, mannose, galactose, maltose, lactose, and sucrose), which might be potential interfering compounds in explosives samples	217:674	This work focuses on the development of a CE method allowing, for the first time, the simultaneous separation of the underivatized first seven cellodextrin oligomers (glucose, cellobiose, cellotriose, cellotetraose, cellopentaose, cellohexaose, and celloheptaose), with a view to analyze the hydrolysates obtained after partial acid depolymerization of nitrocellulose, and eight carbohydrates (ribose, xylose, fructose, mannose, galactose, maltose, lactose, and sucrose), which might be potential interfering compounds in explosives samples.
25820340	6	56	with	25°C	1395:1398	arg1	BGE					1407:1409	a BGE	1405:1409	a BGE composed of 77.4 mM NaOH and 183 mM NaCl	1405:1450	The simultaneous analysis in 20 min total runtime of the 15 carbohydrates plus internal reference (naphthalene sulfonate) was carried out at 25°C with a BGE composed of 77.4 mM NaOH and 183 mM NaCl to adjust the conductivity at the optimum value.
25820340	0	57	with	electrophoresis	69:83	arg1	photochemically					97:111	direct photochemically	90:111	direct photochemically	90:111	Analysis of underivatized cellodextrin oligosaccharides by capillary electrophoresis with direct photochemically induced UV-detection.
25820340	3	58	theme	hexadimethrine	934:947	arg1	bromide					949:955	hexadimethrine bromide	934:955	hexadimethrine bromide	934:955	EOF was reversed to speed up the analysis using a dynamic capillary coating by hexadimethrine bromide.
25820340	4	59	theme	neighboring	1104:1114	arg1	peaks					1116:1120	neighboring peaks	1104:1120	neighboring peaks	1104:1120	A central composite design was carried out to determine the effects of BGE conductivity and sodium hydroxide concentration on resolutions between neighboring peaks, and analysis time.
25820340	6	60	theme	77.4	1423:1426	arg1	mM					1428:1429	mM	1428:1429	mM	1428:1429	The simultaneous analysis in 20 min total runtime of the 15 carbohydrates plus internal reference (naphthalene sulfonate) was carried out at 25°C with a BGE composed of 77.4 mM NaOH and 183 mM NaCl to adjust the conductivity at the optimum value.
25820340	0	61	theme	direct	90:95	arg1	photochemically					97:111	direct photochemically	90:111	direct photochemically	90:111	Analysis of underivatized cellodextrin oligosaccharides by capillary electrophoresis with direct photochemically induced UV-detection.
25820340	6	62	theme	183	1440:1442	arg1	mM					1444:1445	mM	1444:1445	mM	1444:1445	The simultaneous analysis in 20 min total runtime of the 15 carbohydrates plus internal reference (naphthalene sulfonate) was carried out at 25°C with a BGE composed of 77.4 mM NaOH and 183 mM NaCl to adjust the conductivity at the optimum value.
25820340	4	63	theme	composite	968:976	arg1	design					978:983	A central composite design	958:983	A central composite design	958:983	A central composite design was carried out to determine the effects of BGE conductivity and sodium hydroxide concentration on resolutions between neighboring peaks, and analysis time.
25820340	1	64	dep	glucose	302:308	arg1	cellotriose					323:333	cellotriose	323:333	cellotriose	323:333	This work focuses on the development of a CE method allowing, for the first time, the simultaneous separation of the underivatized first seven cellodextrin oligomers (glucose, cellobiose, cellotriose, cellotetraose, cellopentaose, cellohexaose, and celloheptaose), with a view to analyze the hydrolysates obtained after partial acid depolymerization of nitrocellulose, and eight carbohydrates (ribose, xylose, fructose, mannose, galactose, maltose, lactose, and sucrose), which might be potential interfering compounds in explosives samples.
25820340	1	64	dep	glucose	302:308	arg1	cellohexaose					366:377	cellohexaose	366:377	cellohexaose	366:377	This work focuses on the development of a CE method allowing, for the first time, the simultaneous separation of the underivatized first seven cellodextrin oligomers (glucose, cellobiose, cellotriose, cellotetraose, cellopentaose, cellohexaose, and celloheptaose), with a view to analyze the hydrolysates obtained after partial acid depolymerization of nitrocellulose, and eight carbohydrates (ribose, xylose, fructose, mannose, galactose, maltose, lactose, and sucrose), which might be potential interfering compounds in explosives samples.
25820340	1	64	dep	glucose	302:308	arg1	celloheptaose					384:396	celloheptaose	384:396	celloheptaose	384:396	This work focuses on the development of a CE method allowing, for the first time, the simultaneous separation of the underivatized first seven cellodextrin oligomers (glucose, cellobiose, cellotriose, cellotetraose, cellopentaose, cellohexaose, and celloheptaose), with a view to analyze the hydrolysates obtained after partial acid depolymerization of nitrocellulose, and eight carbohydrates (ribose, xylose, fructose, mannose, galactose, maltose, lactose, and sucrose), which might be potential interfering compounds in explosives samples.
25820340	1	64	dep	glucose	302:308	arg1	cellotetraose					336:348	cellotetraose	336:348	cellotetraose	336:348	This work focuses on the development of a CE method allowing, for the first time, the simultaneous separation of the underivatized first seven cellodextrin oligomers (glucose, cellobiose, cellotriose, cellotetraose, cellopentaose, cellohexaose, and celloheptaose), with a view to analyze the hydrolysates obtained after partial acid depolymerization of nitrocellulose, and eight carbohydrates (ribose, xylose, fructose, mannose, galactose, maltose, lactose, and sucrose), which might be potential interfering compounds in explosives samples.
25820340	1	64	dep	glucose	302:308	arg1	cellopentaose					351:363	cellopentaose	351:363	cellopentaose	351:363	This work focuses on the development of a CE method allowing, for the first time, the simultaneous separation of the underivatized first seven cellodextrin oligomers (glucose, cellobiose, cellotriose, cellotetraose, cellopentaose, cellohexaose, and celloheptaose), with a view to analyze the hydrolysates obtained after partial acid depolymerization of nitrocellulose, and eight carbohydrates (ribose, xylose, fructose, mannose, galactose, maltose, lactose, and sucrose), which might be potential interfering compounds in explosives samples.
25820340	1	64	dep	glucose	302:308	arg1	cellobiose					311:320	cellobiose	311:320	cellobiose	311:320	This work focuses on the development of a CE method allowing, for the first time, the simultaneous separation of the underivatized first seven cellodextrin oligomers (glucose, cellobiose, cellotriose, cellotetraose, cellopentaose, cellohexaose, and celloheptaose), with a view to analyze the hydrolysates obtained after partial acid depolymerization of nitrocellulose, and eight carbohydrates (ribose, xylose, fructose, mannose, galactose, maltose, lactose, and sucrose), which might be potential interfering compounds in explosives samples.
25820340	0	65	theme	underivatized	12:24	arg1	oligosaccharides					39:54	underivatized cellodextrin oligosaccharides	12:54	underivatized cellodextrin oligosaccharides	12:54	Analysis of underivatized cellodextrin oligosaccharides by capillary electrophoresis with direct photochemically induced UV-detection.
25820340	5	66	theme	analysis	1239:1246	arg1	time					1248:1251	analysis time	1239:1251	analysis time	1239:1251	A desirability analysis on modeled responses was applied to maximize resolutions and to minimize analysis time.
25820340	4	67	theme	sodium	1050:1055	arg1	concentration					1067:1079	sodium hydroxide concentration	1050:1079	sodium hydroxide concentration	1050:1079	A central composite design was carried out to determine the effects of BGE conductivity and sodium hydroxide concentration on resolutions between neighboring peaks, and analysis time.
26526174	8	0	theme	electrical	1125:1134	arg1	conductivity					1136:1147	appreciable electrical conductivity	1113:1147	appreciable electrical conductivity	1113:1147	GG-g-PANI exhibited appreciable electrical conductivity, which increased with the extent of grafting.
26526174	6	1	theme	mmol	838:841	arg1	acid					878:881	1M hydrochloric acid	862:881	1M hydrochloric acid	862:881	The optimum grafting was observed when the reaction mixture containing 0.066 mmol APS, 0.1M aniline, 1M hydrochloric acid and 0.1g/dL GG was exposed to 80 W microwave power for 40s.
26526174	6	1	theme	mmol	838:841	arg1	GG					895:896	0.1g/dL GG	887:896	0.1g/dL GG	887:896	The optimum grafting was observed when the reaction mixture containing 0.066 mmol APS, 0.1M aniline, 1M hydrochloric acid and 0.1g/dL GG was exposed to 80 W microwave power for 40s.
26526174	6	1	theme	mmol	838:841	arg1	APS					843:845	0.066 mmol APS	832:845	0.066 mmol APS	832:845	The optimum grafting was observed when the reaction mixture containing 0.066 mmol APS, 0.1M aniline, 1M hydrochloric acid and 0.1g/dL GG was exposed to 80 W microwave power for 40s.
26526174	6	1	theme	mmol	838:841	arg1	aniline					853:859	0.1M aniline	848:859	0.1M aniline	848:859	The optimum grafting was observed when the reaction mixture containing 0.066 mmol APS, 0.1M aniline, 1M hydrochloric acid and 0.1g/dL GG was exposed to 80 W microwave power for 40s.
26526174	2	2	theme	microwave	344:352	arg1	time					370:373	the microwave power, exposure time	340:373	time	370:373	The grafting condition was optimized by varying the microwave power, exposure time and the composition of the reaction mixture.
26526174	4	3	theme	characteristic	523:536	arg1	peaks					538:542	The characteristic peaks	519:542	The characteristic peaks at 1506, 1462, 1070 and 830 cm(-1) in the IR spectrum	519:596	The characteristic peaks at 1506, 1462, 1070 and 830 cm(-1) in the IR spectrum and signals at 7.3, 7.2, 7.1 and 4.0 δ in the (1)H NMR spectrum confirms the grafting process.
26526174	4	4	from	1506	547:550	arg1	peaks					538:542	The characteristic peaks	519:542	The characteristic peaks at 1506, 1462, 1070 and 830 cm(-1) in the IR spectrum	519:596	The characteristic peaks at 1506, 1462, 1070 and 830 cm(-1) in the IR spectrum and signals at 7.3, 7.2, 7.1 and 4.0 δ in the (1)H NMR spectrum confirms the grafting process.
26526174	4	4	from	1506	547:550	arg1	signals					602:608	signals	602:608	signals	602:608	The characteristic peaks at 1506, 1462, 1070 and 830 cm(-1) in the IR spectrum and signals at 7.3, 7.2, 7.1 and 4.0 δ in the (1)H NMR spectrum confirms the grafting process.
26526174	9	5	theme	copolymer	1263:1271	arg1	conductivity					1241:1252	DC conductivity	1238:1252	DC conductivity of graft copolymer	1238:1271	The results indicate threefold increase in DC conductivity of graft copolymer as compared to GG.
26526174	3	6	theme	UV/vis	482:487	arg1	techniques					507:516	FTIR, TGA, UV/vis, (1)H NMR and SEM techniques	471:516	FTIR, TGA, UV/vis, (1)H NMR and SEM techniques	471:516	The graft copolymer GG-g-PANI was characterized by FTIR, TGA, UV/vis, (1)H NMR and SEM techniques.
26526174	1	7	theme	mild	233:236	arg1	conditions					245:254	mild acidic conditions	233:254	mild acidic conditions	233:254	Grafting of polyaniline (PANI) on to gellan gum (GG) was carried out in the presence of catalytic amount of ammonium peroxydisulfate (APS) as oxidant/initiator under mild acidic conditions by microwave irradiation technique.
26526174	1	8	theme	polyaniline	79:89	arg1	Grafting					67:74	Grafting	67:74	Grafting of polyaniline (PANI) on to gellan gum (GG)	67:118	Grafting of polyaniline (PANI) on to gellan gum (GG) was carried out in the presence of catalytic amount of ammonium peroxydisulfate (APS) as oxidant/initiator under mild acidic conditions by microwave irradiation technique.
26526174	6	9	theme	1M	862:863	arg1	acid					878:881	1M hydrochloric acid	862:881	1M hydrochloric acid	862:881	The optimum grafting was observed when the reaction mixture containing 0.066 mmol APS, 0.1M aniline, 1M hydrochloric acid and 0.1g/dL GG was exposed to 80 W microwave power for 40s.
26526174	6	9	theme	1M	862:863	arg1	APS					843:845	0.066 mmol APS	832:845	0.066 mmol APS	832:845	The optimum grafting was observed when the reaction mixture containing 0.066 mmol APS, 0.1M aniline, 1M hydrochloric acid and 0.1g/dL GG was exposed to 80 W microwave power for 40s.
26526174	1	10	theme	acidic	238:243	arg1	conditions					245:254	mild acidic conditions	233:254	mild acidic conditions	233:254	Grafting of polyaniline (PANI) on to gellan gum (GG) was carried out in the presence of catalytic amount of ammonium peroxydisulfate (APS) as oxidant/initiator under mild acidic conditions by microwave irradiation technique.
26526174	4	11	from	peaks	538:542	arg1	spectrum					589:596	the IR spectrum	582:596	the IR spectrum	582:596	The characteristic peaks at 1506, 1462, 1070 and 830 cm(-1) in the IR spectrum and signals at 7.3, 7.2, 7.1 and 4.0 δ in the (1)H NMR spectrum confirms the grafting process.
26526174	4	11	from	peaks	538:542	arg1	spectrum					653:660	the (1)H NMR spectrum	640:660	the (1)H NMR spectrum	640:660	The characteristic peaks at 1506, 1462, 1070 and 830 cm(-1) in the IR spectrum and signals at 7.3, 7.2, 7.1 and 4.0 δ in the (1)H NMR spectrum confirms the grafting process.
26526174	4	12	from	δ	635:635	arg1	peaks					538:542	The characteristic peaks	519:542	The characteristic peaks at 1506, 1462, 1070 and 830 cm(-1) in the IR spectrum	519:596	The characteristic peaks at 1506, 1462, 1070 and 830 cm(-1) in the IR spectrum and signals at 7.3, 7.2, 7.1 and 4.0 δ in the (1)H NMR spectrum confirms the grafting process.
26526174	4	12	from	δ	635:635	arg1	signals					602:608	signals	602:608	signals	602:608	The characteristic peaks at 1506, 1462, 1070 and 830 cm(-1) in the IR spectrum and signals at 7.3, 7.2, 7.1 and 4.0 δ in the (1)H NMR spectrum confirms the grafting process.
26526174	3	13	theme	NMR	495:497	arg1	techniques					507:516	FTIR, TGA, UV/vis, (1)H NMR and SEM techniques	471:516	FTIR, TGA, UV/vis, (1)H NMR and SEM techniques	471:516	The graft copolymer GG-g-PANI was characterized by FTIR, TGA, UV/vis, (1)H NMR and SEM techniques.
26526174	6	14	theme	microwave	918:926	arg1	power					928:932	80 W microwave power	913:932	80 W microwave power	913:932	The optimum grafting was observed when the reaction mixture containing 0.066 mmol APS, 0.1M aniline, 1M hydrochloric acid and 0.1g/dL GG was exposed to 80 W microwave power for 40s.
26526174	7	15	theme	GG-g-PANI	977:985	arg1	DC					947:948	DC	947:948	DC	947:948	The DC and AC conductivity of the GG-g-PANI were measured using the 'Two-point probe' method based on which the dielectric properties were evaluated.
26526174	7	15	theme	GG-g-PANI	977:985	arg1	conductivity					957:968	AC conductivity	954:968	AC conductivity	954:968	The DC and AC conductivity of the GG-g-PANI were measured using the 'Two-point probe' method based on which the dielectric properties were evaluated.
26526174	6	16	theme	hydrochloric	865:876	arg1	acid					878:881	1M hydrochloric acid	862:881	1M hydrochloric acid	862:881	The optimum grafting was observed when the reaction mixture containing 0.066 mmol APS, 0.1M aniline, 1M hydrochloric acid and 0.1g/dL GG was exposed to 80 W microwave power for 40s.
26526174	6	16	theme	hydrochloric	865:876	arg1	APS					843:845	0.066 mmol APS	832:845	0.066 mmol APS	832:845	The optimum grafting was observed when the reaction mixture containing 0.066 mmol APS, 0.1M aniline, 1M hydrochloric acid and 0.1g/dL GG was exposed to 80 W microwave power for 40s.
26526174	6	17	theme	0.1g/dL	887:893	arg1	GG					895:896	0.1g/dL GG	887:896	0.1g/dL GG	887:896	The optimum grafting was observed when the reaction mixture containing 0.066 mmol APS, 0.1M aniline, 1M hydrochloric acid and 0.1g/dL GG was exposed to 80 W microwave power for 40s.
26526174	6	17	theme	0.1g/dL	887:893	arg1	APS					843:845	0.066 mmol APS	832:845	0.066 mmol APS	832:845	The optimum grafting was observed when the reaction mixture containing 0.066 mmol APS, 0.1M aniline, 1M hydrochloric acid and 0.1g/dL GG was exposed to 80 W microwave power for 40s.
26526174	5	18	theme	TGA	697:699	arg1	data					701:704	The TGA data	693:704	The TGA data	693:704	The TGA data reveals GG-g-PANI to be thermally less stable than GG.
26526174	9	19	theme	DC	1238:1239	arg1	conductivity					1241:1252	DC conductivity	1238:1252	DC conductivity of graft copolymer	1238:1271	The results indicate threefold increase in DC conductivity of graft copolymer as compared to GG.
26526174	4	20	theme	grafting	675:682	arg1	process					684:690	the grafting process	671:690	the grafting process	671:690	The characteristic peaks at 1506, 1462, 1070 and 830 cm(-1) in the IR spectrum and signals at 7.3, 7.2, 7.1 and 4.0 δ in the (1)H NMR spectrum confirms the grafting process.
26526174	6	21	theme	W	916:916	arg1	power					928:932	80 W microwave power	913:932	80 W microwave power	913:932	The optimum grafting was observed when the reaction mixture containing 0.066 mmol APS, 0.1M aniline, 1M hydrochloric acid and 0.1g/dL GG was exposed to 80 W microwave power for 40s.
26526174	3	22	theme	graft	424:428	arg1	GG-g-PANI					440:448	The graft copolymer GG-g-PANI	420:448	The graft copolymer GG-g-PANI	420:448	The graft copolymer GG-g-PANI was characterized by FTIR, TGA, UV/vis, (1)H NMR and SEM techniques.
26526174	7	23	dep	DC	947:948	arg1	The					943:945	The	943:945	The	943:945	The DC and AC conductivity of the GG-g-PANI were measured using the 'Two-point probe' method based on which the dielectric properties were evaluated.
26526174	1	24	theme	catalytic	155:163	arg1	APS					201:203	APS	201:203	APS	201:203	Grafting of polyaniline (PANI) on to gellan gum (GG) was carried out in the presence of catalytic amount of ammonium peroxydisulfate (APS) as oxidant/initiator under mild acidic conditions by microwave irradiation technique.
26526174	1	24	theme	catalytic	155:163	arg1	amount					165:170	catalytic amount	155:170	catalytic amount of ammonium peroxydisulfate (APS) as oxidant/initiator under mild acidic conditions	155:254	Grafting of polyaniline (PANI) on to gellan gum (GG) was carried out in the presence of catalytic amount of ammonium peroxydisulfate (APS) as oxidant/initiator under mild acidic conditions by microwave irradiation technique.
26526174	1	24	theme	catalytic	155:163	arg1	peroxydisulfate					184:198	ammonium peroxydisulfate	175:198	ammonium peroxydisulfate (APS) as oxidant/initiator under mild acidic conditions	175:254	Grafting of polyaniline (PANI) on to gellan gum (GG) was carried out in the presence of catalytic amount of ammonium peroxydisulfate (APS) as oxidant/initiator under mild acidic conditions by microwave irradiation technique.
26526174	6	25	theme	reaction	804:811	arg1	mixture					813:819	the reaction mixture	800:819	the reaction mixture containing 0.066 mmol APS, 0.1M aniline, 1M hydrochloric acid and 0.1g/dL GG	800:896	The optimum grafting was observed when the reaction mixture containing 0.066 mmol APS, 0.1M aniline, 1M hydrochloric acid and 0.1g/dL GG was exposed to 80 W microwave power for 40s.
26526174	9	26	dep	increase	1226:1233	arg1	compared					1276:1283	compared	1276:1283	compared to GG	1276:1289	The results indicate threefold increase in DC conductivity of graft copolymer as compared to GG.
26526174	2	27	theme	grafting	296:303	arg1	condition					305:313	The grafting condition	292:313	The grafting condition	292:313	The grafting condition was optimized by varying the microwave power, exposure time and the composition of the reaction mixture.
26526174	4	28	from	cm	572:573	arg1	peaks					538:542	The characteristic peaks	519:542	The characteristic peaks at 1506, 1462, 1070 and 830 cm(-1) in the IR spectrum	519:596	The characteristic peaks at 1506, 1462, 1070 and 830 cm(-1) in the IR spectrum and signals at 7.3, 7.2, 7.1 and 4.0 δ in the (1)H NMR spectrum confirms the grafting process.
26526174	4	28	from	cm	572:573	arg1	signals					602:608	signals	602:608	signals	602:608	The characteristic peaks at 1506, 1462, 1070 and 830 cm(-1) in the IR spectrum and signals at 7.3, 7.2, 7.1 and 4.0 δ in the (1)H NMR spectrum confirms the grafting process.
26526174	1	29	theme	amount	165:170	arg1	presence					143:150	the presence	139:150	the presence of catalytic amount of ammonium peroxydisulfate (APS) as oxidant/initiator under mild acidic conditions	139:254	Grafting of polyaniline (PANI) on to gellan gum (GG) was carried out in the presence of catalytic amount of ammonium peroxydisulfate (APS) as oxidant/initiator under mild acidic conditions by microwave irradiation technique.
26526174	0	30	theme	conducting	44:53	arg1	biopolymer					55:64	electrical conducting biopolymer	33:64	electrical conducting biopolymer	33:64	Gellan gum-graft-polyaniline--An electrical conducting biopolymer.
26526174	1	31	theme	microwave	259:267	arg1	irradiation					269:279	microwave irradiation	259:279	microwave irradiation technique	259:289	Grafting of polyaniline (PANI) on to gellan gum (GG) was carried out in the presence of catalytic amount of ammonium peroxydisulfate (APS) as oxidant/initiator under mild acidic conditions by microwave irradiation technique.
26526174	1	32	theme	gellan	104:109	arg1	GG					116:117	GG	116:117	GG	116:117	Grafting of polyaniline (PANI) on to gellan gum (GG) was carried out in the presence of catalytic amount of ammonium peroxydisulfate (APS) as oxidant/initiator under mild acidic conditions by microwave irradiation technique.
26526174	1	32	theme	gellan	104:109	arg1	gum					111:113	gellan gum	104:113	gellan gum (GG)	104:118	Grafting of polyaniline (PANI) on to gellan gum (GG) was carried out in the presence of catalytic amount of ammonium peroxydisulfate (APS) as oxidant/initiator under mild acidic conditions by microwave irradiation technique.
26526174	2	33	theme	reaction	402:409	arg1	mixture					411:417	the reaction mixture	398:417	the reaction mixture	398:417	The grafting condition was optimized by varying the microwave power, exposure time and the composition of the reaction mixture.
26526174	2	34	theme	mixture	411:417	arg1	time					370:373	the microwave power, exposure time	340:373	time	370:373	The grafting condition was optimized by varying the microwave power, exposure time and the composition of the reaction mixture.
26526174	2	34	theme	mixture	411:417	arg1	composition					383:393	the composition	379:393	the composition of the reaction mixture	379:417	The grafting condition was optimized by varying the microwave power, exposure time and the composition of the reaction mixture.
26526174	1	35	theme	irradiation	269:279	arg1	technique					281:289	microwave irradiation technique	259:289	microwave irradiation technique	259:289	Grafting of polyaniline (PANI) on to gellan gum (GG) was carried out in the presence of catalytic amount of ammonium peroxydisulfate (APS) as oxidant/initiator under mild acidic conditions by microwave irradiation technique.
26526174	3	36	theme	SEM	503:505	arg1	techniques					507:516	FTIR, TGA, UV/vis, (1)H NMR and SEM techniques	471:516	FTIR, TGA, UV/vis, (1)H NMR and SEM techniques	471:516	The graft copolymer GG-g-PANI was characterized by FTIR, TGA, UV/vis, (1)H NMR and SEM techniques.
26526174	1	37	theme	ammonium	175:182	arg1	APS					201:203	APS	201:203	APS	201:203	Grafting of polyaniline (PANI) on to gellan gum (GG) was carried out in the presence of catalytic amount of ammonium peroxydisulfate (APS) as oxidant/initiator under mild acidic conditions by microwave irradiation technique.
26526174	1	37	theme	ammonium	175:182	arg1	peroxydisulfate					184:198	ammonium peroxydisulfate	175:198	ammonium peroxydisulfate (APS) as oxidant/initiator under mild acidic conditions	175:254	Grafting of polyaniline (PANI) on to gellan gum (GG) was carried out in the presence of catalytic amount of ammonium peroxydisulfate (APS) as oxidant/initiator under mild acidic conditions by microwave irradiation technique.
26526174	3	38	theme	copolymer	430:438	arg1	GG-g-PANI					440:448	The graft copolymer GG-g-PANI	420:448	The graft copolymer GG-g-PANI	420:448	The graft copolymer GG-g-PANI was characterized by FTIR, TGA, UV/vis, (1)H NMR and SEM techniques.
26526174	8	39	theme	appreciable	1113:1123	arg1	conductivity					1136:1147	appreciable electrical conductivity	1113:1147	appreciable electrical conductivity	1113:1147	GG-g-PANI exhibited appreciable electrical conductivity, which increased with the extent of grafting.
26526174	4	40	from	1070	559:562	arg1	peaks					538:542	The characteristic peaks	519:542	The characteristic peaks at 1506, 1462, 1070 and 830 cm(-1) in the IR spectrum	519:596	The characteristic peaks at 1506, 1462, 1070 and 830 cm(-1) in the IR spectrum and signals at 7.3, 7.2, 7.1 and 4.0 δ in the (1)H NMR spectrum confirms the grafting process.
26526174	4	40	from	1070	559:562	arg1	signals					602:608	signals	602:608	signals	602:608	The characteristic peaks at 1506, 1462, 1070 and 830 cm(-1) in the IR spectrum and signals at 7.3, 7.2, 7.1 and 4.0 δ in the (1)H NMR spectrum confirms the grafting process.
26526174	1	41	theme	peroxydisulfate	184:198	arg1	APS					201:203	APS	201:203	APS	201:203	Grafting of polyaniline (PANI) on to gellan gum (GG) was carried out in the presence of catalytic amount of ammonium peroxydisulfate (APS) as oxidant/initiator under mild acidic conditions by microwave irradiation technique.
26526174	1	41	theme	peroxydisulfate	184:198	arg1	amount					165:170	catalytic amount	155:170	catalytic amount of ammonium peroxydisulfate (APS) as oxidant/initiator under mild acidic conditions	155:254	Grafting of polyaniline (PANI) on to gellan gum (GG) was carried out in the presence of catalytic amount of ammonium peroxydisulfate (APS) as oxidant/initiator under mild acidic conditions by microwave irradiation technique.
26526174	1	41	theme	peroxydisulfate	184:198	arg1	peroxydisulfate					184:198	ammonium peroxydisulfate	175:198	ammonium peroxydisulfate (APS) as oxidant/initiator under mild acidic conditions	175:254	Grafting of polyaniline (PANI) on to gellan gum (GG) was carried out in the presence of catalytic amount of ammonium peroxydisulfate (APS) as oxidant/initiator under mild acidic conditions by microwave irradiation technique.
26526174	9	42	from	increase	1226:1233	arg1	conductivity					1241:1252	DC conductivity	1238:1252	DC conductivity of graft copolymer	1238:1271	The results indicate threefold increase in DC conductivity of graft copolymer as compared to GG.
26526174	4	43	theme	NMR	649:651	arg1	spectrum					653:660	the (1)H NMR spectrum	640:660	the (1)H NMR spectrum	640:660	The characteristic peaks at 1506, 1462, 1070 and 830 cm(-1) in the IR spectrum and signals at 7.3, 7.2, 7.1 and 4.0 δ in the (1)H NMR spectrum confirms the grafting process.
26526174	9	44	theme	threefold	1216:1224	arg1	increase					1226:1233	threefold increase	1216:1233	threefold increase in DC conductivity of graft copolymer as compared to GG	1216:1289	The results indicate threefold increase in DC conductivity of graft copolymer as compared to GG.
26526174	6	45	contain	containing	821:830	arg2	aniline					853:859	0.1M aniline	848:859	0.1M aniline	848:859	The optimum grafting was observed when the reaction mixture containing 0.066 mmol APS, 0.1M aniline, 1M hydrochloric acid and 0.1g/dL GG was exposed to 80 W microwave power for 40s.
26526174	6	45	contain	containing	821:830	arg1	mixture					813:819	the reaction mixture	800:819	the reaction mixture containing 0.066 mmol APS, 0.1M aniline, 1M hydrochloric acid and 0.1g/dL GG	800:896	The optimum grafting was observed when the reaction mixture containing 0.066 mmol APS, 0.1M aniline, 1M hydrochloric acid and 0.1g/dL GG was exposed to 80 W microwave power for 40s.
26526174	6	45	contain	containing	821:830	arg2	GG					895:896	0.1g/dL GG	887:896	0.1g/dL GG	887:896	The optimum grafting was observed when the reaction mixture containing 0.066 mmol APS, 0.1M aniline, 1M hydrochloric acid and 0.1g/dL GG was exposed to 80 W microwave power for 40s.
26526174	6	45	contain	containing	821:830	arg2	acid					878:881	1M hydrochloric acid	862:881	1M hydrochloric acid	862:881	The optimum grafting was observed when the reaction mixture containing 0.066 mmol APS, 0.1M aniline, 1M hydrochloric acid and 0.1g/dL GG was exposed to 80 W microwave power for 40s.
26526174	6	45	contain	containing	821:830	arg2	APS					843:845	0.066 mmol APS	832:845	0.066 mmol APS	832:845	The optimum grafting was observed when the reaction mixture containing 0.066 mmol APS, 0.1M aniline, 1M hydrochloric acid and 0.1g/dL GG was exposed to 80 W microwave power for 40s.
26526174	7	46	theme	dielectric	1055:1064	arg1	properties					1066:1075	the dielectric properties	1051:1075	the dielectric properties	1051:1075	The DC and AC conductivity of the GG-g-PANI were measured using the 'Two-point probe' method based on which the dielectric properties were evaluated.
26526174	6	47	theme	optimum	765:771	arg1	grafting					773:780	The optimum grafting	761:780	The optimum grafting	761:780	The optimum grafting was observed when the reaction mixture containing 0.066 mmol APS, 0.1M aniline, 1M hydrochloric acid and 0.1g/dL GG was exposed to 80 W microwave power for 40s.
26526174	3	48	theme	TGA	477:479	arg1	techniques					507:516	FTIR, TGA, UV/vis, (1)H NMR and SEM techniques	471:516	FTIR, TGA, UV/vis, (1)H NMR and SEM techniques	471:516	The graft copolymer GG-g-PANI was characterized by FTIR, TGA, UV/vis, (1)H NMR and SEM techniques.
26526174	7	49	theme	AC	954:955	arg1	conductivity					957:968	AC conductivity	954:968	AC conductivity	954:968	The DC and AC conductivity of the GG-g-PANI were measured using the 'Two-point probe' method based on which the dielectric properties were evaluated.
26526174	2	50	dep	time	370:373	arg1	exposure					361:368	the microwave power, exposure time	340:373	exposure	361:368	The grafting condition was optimized by varying the microwave power, exposure time and the composition of the reaction mixture.
26526174	8	51	theme	grafting	1185:1192	arg1	extent					1175:1180	the extent	1171:1180	the extent of grafting	1171:1192	GG-g-PANI exhibited appreciable electrical conductivity, which increased with the extent of grafting.
26526174	3	52	theme	H	493:493	arg1	NMR					495:497	(1)H NMR	490:497	(1)H NMR	490:497	The graft copolymer GG-g-PANI was characterized by FTIR, TGA, UV/vis, (1)H NMR and SEM techniques.
26526174	3	53	theme	FTIR	471:474	arg1	techniques					507:516	FTIR, TGA, UV/vis, (1)H NMR and SEM techniques	471:516	FTIR, TGA, UV/vis, (1)H NMR and SEM techniques	471:516	The graft copolymer GG-g-PANI was characterized by FTIR, TGA, UV/vis, (1)H NMR and SEM techniques.
26526174	6	54	theme	0.1M	848:851	arg1	APS					843:845	0.066 mmol APS	832:845	0.066 mmol APS	832:845	The optimum grafting was observed when the reaction mixture containing 0.066 mmol APS, 0.1M aniline, 1M hydrochloric acid and 0.1g/dL GG was exposed to 80 W microwave power for 40s.
26526174	6	54	theme	0.1M	848:851	arg1	aniline					853:859	0.1M aniline	848:859	0.1M aniline	848:859	The optimum grafting was observed when the reaction mixture containing 0.066 mmol APS, 0.1M aniline, 1M hydrochloric acid and 0.1g/dL GG was exposed to 80 W microwave power for 40s.
26526174	7	55	theme	Two-point	1012:1020	arg1	method					1029:1034	the 'Two-point probe' method	1007:1034	the 'Two-point probe' method based on which the dielectric properties were evaluated	1007:1090	The DC and AC conductivity of the GG-g-PANI were measured using the 'Two-point probe' method based on which the dielectric properties were evaluated.
26526174	6	56	theme	80	913:914	arg1	W					916:916	W	916:916	W	916:916	The optimum grafting was observed when the reaction mixture containing 0.066 mmol APS, 0.1M aniline, 1M hydrochloric acid and 0.1g/dL GG was exposed to 80 W microwave power for 40s.
26526174	4	57	theme	H	647:647	arg1	spectrum					653:660	the (1)H NMR spectrum	640:660	the (1)H NMR spectrum	640:660	The characteristic peaks at 1506, 1462, 1070 and 830 cm(-1) in the IR spectrum and signals at 7.3, 7.2, 7.1 and 4.0 δ in the (1)H NMR spectrum confirms the grafting process.
26526174	4	58	theme	IR	586:587	arg1	spectrum					589:596	the IR spectrum	582:596	the IR spectrum	582:596	The characteristic peaks at 1506, 1462, 1070 and 830 cm(-1) in the IR spectrum and signals at 7.3, 7.2, 7.1 and 4.0 δ in the (1)H NMR spectrum confirms the grafting process.
26526174	4	59	from	signals	602:608	arg1	spectrum					589:596	the IR spectrum	582:596	the IR spectrum	582:596	The characteristic peaks at 1506, 1462, 1070 and 830 cm(-1) in the IR spectrum and signals at 7.3, 7.2, 7.1 and 4.0 δ in the (1)H NMR spectrum confirms the grafting process.
26526174	4	59	from	signals	602:608	arg1	spectrum					653:660	the (1)H NMR spectrum	640:660	the (1)H NMR spectrum	640:660	The characteristic peaks at 1506, 1462, 1070 and 830 cm(-1) in the IR spectrum and signals at 7.3, 7.2, 7.1 and 4.0 δ in the (1)H NMR spectrum confirms the grafting process.
26526174	4	60	from	1462	553:556	arg1	peaks					538:542	The characteristic peaks	519:542	The characteristic peaks at 1506, 1462, 1070 and 830 cm(-1) in the IR spectrum	519:596	The characteristic peaks at 1506, 1462, 1070 and 830 cm(-1) in the IR spectrum and signals at 7.3, 7.2, 7.1 and 4.0 δ in the (1)H NMR spectrum confirms the grafting process.
26526174	4	60	from	1462	553:556	arg1	signals					602:608	signals	602:608	signals	602:608	The characteristic peaks at 1506, 1462, 1070 and 830 cm(-1) in the IR spectrum and signals at 7.3, 7.2, 7.1 and 4.0 δ in the (1)H NMR spectrum confirms the grafting process.
26526174	9	61	theme	graft	1257:1261	arg1	copolymer					1263:1271	graft copolymer	1257:1271	graft copolymer	1257:1271	The results indicate threefold increase in DC conductivity of graft copolymer as compared to GG.
26526174	0	62	theme	electrical	33:42	arg1	biopolymer					55:64	electrical conducting biopolymer	33:64	electrical conducting biopolymer	33:64	Gellan gum-graft-polyaniline--An electrical conducting biopolymer.
26526174	2	63	theme	power	354:358	arg1	time					370:373	the microwave power, exposure time	340:373	time	370:373	The grafting condition was optimized by varying the microwave power, exposure time and the composition of the reaction mixture.
28522391	0	0	theme	cell	83:86	arg1	activation					88:97	macrophage and NK cell activation	65:97	activation	88:97	Polysaccharides from ginseng leaves inhibit tumor metastasis via macrophage and NK cell activation.
28522391	0	1	from	ginseng	21:27	arg1	Polysaccharides					0:14	Polysaccharides	0:14	Polysaccharides from ginseng leaves	0:34	Polysaccharides from ginseng leaves inhibit tumor metastasis via macrophage and NK cell activation.
28522391	2	2	theme	10.2kDa	324:330	arg1	GS-P					314:317	GS-P	314:317	GS-P	314:317	GS-P is a 10.2kDa pectic polysaccharide consisting of 15 different monosaccharides.
28522391	2	2	theme	10.2kDa	324:330	arg1	polysaccharide					339:352	a 10.2kDa pectic polysaccharide	322:352	a 10.2kDa pectic polysaccharide consisting of 15 different monosaccharides	322:395	GS-P is a 10.2kDa pectic polysaccharide consisting of 15 different monosaccharides.
28522391	0	3	theme	NK	80:81	arg1	activation					88:97	macrophage and NK cell activation	65:97	activation	88:97	Polysaccharides from ginseng leaves inhibit tumor metastasis via macrophage and NK cell activation.
28522391	5	4	theme	IL	637:638	arg1	Secretion					577:585	Secretion	577:585	Secretion of tumor necrosis factor (TNF)-α and interleukin (IL)-12	577:642	Secretion of tumor necrosis factor (TNF)-α and interleukin (IL)-12 was enhanced in the peritoneal exudate macrophages (PEMs) of GS-P-treated mice.
28522391	5	5	theme	necrosis	596:603	arg1	factor					605:610	tumor necrosis factor	590:610	tumor necrosis factor (TNF)-α	590:618	Secretion of tumor necrosis factor (TNF)-α and interleukin (IL)-12 was enhanced in the peritoneal exudate macrophages (PEMs) of GS-P-treated mice.
28522391	5	5	theme	necrosis	596:603	arg1	TNF					613:615	TNF	613:615	TNF	613:615	Secretion of tumor necrosis factor (TNF)-α and interleukin (IL)-12 was enhanced in the peritoneal exudate macrophages (PEMs) of GS-P-treated mice.
28522391	1	6	theme	monosaccharide	223:236	arg1	composition					238:248	its monosaccharide composition	219:248	its monosaccharide composition	219:248	In this study, we purified the polysaccharide fraction (GS-P) from the leaves of Panax ginseng C.A. Meyer and analyzed its monosaccharide composition and antitumor and antimetastatic activity in vitro and in vivo.
28522391	5	7	theme	factor	605:610	arg1	-α					617:618	tumor necrosis factor (TNF)-α	590:618	tumor necrosis factor (TNF)-α	590:618	Secretion of tumor necrosis factor (TNF)-α and interleukin (IL)-12 was enhanced in the peritoneal exudate macrophages (PEMs) of GS-P-treated mice.
28522391	7	8	from	effects	1062:1068	arg1	metastasis					1086:1095	lung metastasis	1081:1095	lung metastasis	1081:1095	Pretreatment with anti-asialo GM1 (an antibody for NK cell depletion) partly suppressed the inhibitory effects of GS-P on lung metastasis.
28522391	6	9	from	mice	883:886	arg1	splenocytes					853:863	splenocytes	853:863	splenocytes from GS-P-treated mice	853:886	Moreover, PEMs obtained from GS-P-treated mice showed significantly higher tumoricidal activity against colon 26-M3.1 cells, and splenocytes from GS-P-treated mice significantly enhanced NK cell cytotoxicity against YAC-1 tumor cells.
28522391	6	10	theme	GS-P-treated	870:881	arg1	mice					883:886	GS-P-treated mice	870:886	GS-P-treated mice	870:886	Moreover, PEMs obtained from GS-P-treated mice showed significantly higher tumoricidal activity against colon 26-M3.1 cells, and splenocytes from GS-P-treated mice significantly enhanced NK cell cytotoxicity against YAC-1 tumor cells.
28522391	7	11	theme	lung	1081:1084	arg1	metastasis					1086:1095	lung metastasis	1081:1095	lung metastasis	1081:1095	Pretreatment with anti-asialo GM1 (an antibody for NK cell depletion) partly suppressed the inhibitory effects of GS-P on lung metastasis.
28522391	2	12	theme	different	371:379	arg1	monosaccharides					381:395	15 different monosaccharides	368:395	15 different monosaccharides	368:395	GS-P is a 10.2kDa pectic polysaccharide consisting of 15 different monosaccharides.
28522391	5	13	theme	GS-P-treated	705:716	arg1	mice					718:721	GS-P-treated mice	705:721	GS-P-treated mice	705:721	Secretion of tumor necrosis factor (TNF)-α and interleukin (IL)-12 was enhanced in the peritoneal exudate macrophages (PEMs) of GS-P-treated mice.
28522391	0	14	theme	tumor	44:48	arg1	metastasis					50:59	tumor metastasis	44:59	tumor metastasis	44:59	Polysaccharides from ginseng leaves inhibit tumor metastasis via macrophage and NK cell activation.
28522391	6	15	theme	GS-P-treated	753:764	arg1	mice					766:769	GS-P-treated mice	753:769	GS-P-treated mice	753:769	Moreover, PEMs obtained from GS-P-treated mice showed significantly higher tumoricidal activity against colon 26-M3.1 cells, and splenocytes from GS-P-treated mice significantly enhanced NK cell cytotoxicity against YAC-1 tumor cells.
28522391	7	16	theme	GS-P	1073:1076	arg1	effects					1062:1068	the inhibitory effects	1047:1068	the inhibitory effects of GS-P on lung metastasis	1047:1095	Pretreatment with anti-asialo GM1 (an antibody for NK cell depletion) partly suppressed the inhibitory effects of GS-P on lung metastasis.
28522391	1	17	theme	antitumor	254:262	arg1	activity					283:290	antitumor and antimetastatic activity	254:290	antitumor and antimetastatic activity	254:290	In this study, we purified the polysaccharide fraction (GS-P) from the leaves of Panax ginseng C.A. Meyer and analyzed its monosaccharide composition and antitumor and antimetastatic activity in vitro and in vivo.
28522391	7	18	dep	GM1	989:991	arg1	antibody					997:1004	an antibody	994:1004	an antibody for NK cell depletion	994:1026	Pretreatment with anti-asialo GM1 (an antibody for NK cell depletion) partly suppressed the inhibitory effects of GS-P on lung metastasis.
28522391	4	19	theme	mouse	545:549	arg1	splenocyte					551:560	mouse splenocyte	545:560	mouse splenocyte proliferation	545:574	GS-P was not cytotoxic to colon 26-M3.1 cells and increased mouse splenocyte proliferation.
28522391	5	20	theme	interleukin	624:634	arg1	IL					637:638	interleukin (IL)-12	624:642	interleukin (IL)-12	624:642	Secretion of tumor necrosis factor (TNF)-α and interleukin (IL)-12 was enhanced in the peritoneal exudate macrophages (PEMs) of GS-P-treated mice.
28522391	8	21	theme	antimetastatic	1136:1149	arg1	activity					1151:1158	antimetastatic activity	1136:1158	antimetastatic activity	1136:1158	These data suggest that GS-P exhibits antimetastatic activity by promoting the activation of macrophages and NK cells.
28522391	5	22	theme	peritoneal	664:673	arg1	PEMs					696:699	PEMs	696:699	PEMs	696:699	Secretion of tumor necrosis factor (TNF)-α and interleukin (IL)-12 was enhanced in the peritoneal exudate macrophages (PEMs) of GS-P-treated mice.
28522391	5	22	theme	peritoneal	664:673	arg1	macrophages					683:693	the peritoneal exudate macrophages	660:693	the peritoneal exudate macrophages (PEMs) of GS-P-treated mice	660:721	Secretion of tumor necrosis factor (TNF)-α and interleukin (IL)-12 was enhanced in the peritoneal exudate macrophages (PEMs) of GS-P-treated mice.
28522391	5	23	theme	tumor	590:594	arg1	factor					605:610	tumor necrosis factor	590:610	tumor necrosis factor (TNF)-α	590:618	Secretion of tumor necrosis factor (TNF)-α and interleukin (IL)-12 was enhanced in the peritoneal exudate macrophages (PEMs) of GS-P-treated mice.
28522391	5	23	theme	tumor	590:594	arg1	TNF					613:615	TNF	613:615	TNF	613:615	Secretion of tumor necrosis factor (TNF)-α and interleukin (IL)-12 was enhanced in the peritoneal exudate macrophages (PEMs) of GS-P-treated mice.
28522391	8	24	theme	cells	1210:1214	arg1	activation					1177:1186	the activation	1173:1186	the activation of macrophages and NK cells	1173:1214	These data suggest that GS-P exhibits antimetastatic activity by promoting the activation of macrophages and NK cells.
28522391	6	25	theme	higher	792:797	arg1	activity					811:818	significantly higher tumoricidal activity	778:818	significantly higher tumoricidal activity against colon 26-M3.1 cells	778:846	Moreover, PEMs obtained from GS-P-treated mice showed significantly higher tumoricidal activity against colon 26-M3.1 cells, and splenocytes from GS-P-treated mice significantly enhanced NK cell cytotoxicity against YAC-1 tumor cells.
28522391	8	26	theme	macrophages	1191:1201	arg1	activation					1177:1186	the activation	1173:1186	the activation of macrophages and NK cells	1173:1214	These data suggest that GS-P exhibits antimetastatic activity by promoting the activation of macrophages and NK cells.
28522391	1	27	theme	antimetastatic	268:281	arg1	activity					283:290	antitumor and antimetastatic activity	254:290	antitumor and antimetastatic activity	254:290	In this study, we purified the polysaccharide fraction (GS-P) from the leaves of Panax ginseng C.A. Meyer and analyzed its monosaccharide composition and antitumor and antimetastatic activity in vitro and in vivo.
28522391	5	28	theme	exudate	675:681	arg1	PEMs					696:699	PEMs	696:699	PEMs	696:699	Secretion of tumor necrosis factor (TNF)-α and interleukin (IL)-12 was enhanced in the peritoneal exudate macrophages (PEMs) of GS-P-treated mice.
28522391	5	28	theme	exudate	675:681	arg1	macrophages					683:693	the peritoneal exudate macrophages	660:693	the peritoneal exudate macrophages (PEMs) of GS-P-treated mice	660:721	Secretion of tumor necrosis factor (TNF)-α and interleukin (IL)-12 was enhanced in the peritoneal exudate macrophages (PEMs) of GS-P-treated mice.
28522391	6	29	theme	NK	911:912	arg1	cytotoxicity					919:930	NK cell cytotoxicity	911:930	NK cell cytotoxicity	911:930	Moreover, PEMs obtained from GS-P-treated mice showed significantly higher tumoricidal activity against colon 26-M3.1 cells, and splenocytes from GS-P-treated mice significantly enhanced NK cell cytotoxicity against YAC-1 tumor cells.
28522391	3	30	theme	GS-P	398:401	arg1	treatment					403:411	GS-P treatment	398:411	GS-P treatment	398:411	GS-P treatment significantly inhibited metastasis in mice, in a dose-dependent manner.
28522391	4	31	theme	splenocyte	551:560	arg1	proliferation					562:574	mouse splenocyte proliferation	545:574	mouse splenocyte proliferation	545:574	GS-P was not cytotoxic to colon 26-M3.1 cells and increased mouse splenocyte proliferation.
28522391	8	32	theme	NK	1207:1208	arg1	cells					1210:1214	NK cells	1207:1214	NK cells	1207:1214	These data suggest that GS-P exhibits antimetastatic activity by promoting the activation of macrophages and NK cells.
28522391	4	33	theme	26-M3.1	517:523	arg1	cells					525:529	colon 26-M3.1 cells	511:529	colon 26-M3.1 cells	511:529	GS-P was not cytotoxic to colon 26-M3.1 cells and increased mouse splenocyte proliferation.
28522391	7	34	theme	inhibitory	1051:1060	arg1	effects					1062:1068	the inhibitory effects	1047:1068	the inhibitory effects of GS-P on lung metastasis	1047:1095	Pretreatment with anti-asialo GM1 (an antibody for NK cell depletion) partly suppressed the inhibitory effects of GS-P on lung metastasis.
28522391	1	35	dep	the	167:169	arg1	leaves					171:176	leaves	171:176	leaves	171:176	In this study, we purified the polysaccharide fraction (GS-P) from the leaves of Panax ginseng C.A. Meyer and analyzed its monosaccharide composition and antitumor and antimetastatic activity in vitro and in vivo.
28522391	7	36	theme	NK	1010:1011	arg1	depletion					1018:1026	NK cell depletion	1010:1026	NK cell depletion	1010:1026	Pretreatment with anti-asialo GM1 (an antibody for NK cell depletion) partly suppressed the inhibitory effects of GS-P on lung metastasis.
28522391	4	37	theme	colon	511:515	arg1	cells					525:529	colon 26-M3.1 cells	511:529	colon 26-M3.1 cells	511:529	GS-P was not cytotoxic to colon 26-M3.1 cells and increased mouse splenocyte proliferation.
28522391	6	38	theme	26-M3.1	834:840	arg1	cells					842:846	colon 26-M3.1 cells	828:846	colon 26-M3.1 cells	828:846	Moreover, PEMs obtained from GS-P-treated mice showed significantly higher tumoricidal activity against colon 26-M3.1 cells, and splenocytes from GS-P-treated mice significantly enhanced NK cell cytotoxicity against YAC-1 tumor cells.
28522391	7	39	theme	cell	1013:1016	arg1	depletion					1018:1026	NK cell depletion	1010:1026	NK cell depletion	1010:1026	Pretreatment with anti-asialo GM1 (an antibody for NK cell depletion) partly suppressed the inhibitory effects of GS-P on lung metastasis.
28522391	7	40	with	Pretreatment	959:970	arg1	GM1					989:991	anti-asialo GM1	977:991	anti-asialo GM1 (an antibody for NK cell depletion)	977:1027	Pretreatment with anti-asialo GM1 (an antibody for NK cell depletion) partly suppressed the inhibitory effects of GS-P on lung metastasis.
28522391	6	41	theme	colon	828:832	arg1	cells					842:846	colon 26-M3.1 cells	828:846	colon 26-M3.1 cells	828:846	Moreover, PEMs obtained from GS-P-treated mice showed significantly higher tumoricidal activity against colon 26-M3.1 cells, and splenocytes from GS-P-treated mice significantly enhanced NK cell cytotoxicity against YAC-1 tumor cells.
28522391	5	42	theme	-α	617:618	arg1	Secretion					577:585	Secretion	577:585	Secretion of tumor necrosis factor (TNF)-α and interleukin (IL)-12	577:642	Secretion of tumor necrosis factor (TNF)-α and interleukin (IL)-12 was enhanced in the peritoneal exudate macrophages (PEMs) of GS-P-treated mice.
28522391	6	43	theme	tumor	946:950	arg1	cells					952:956	YAC-1 tumor cells	940:956	YAC-1 tumor cells	940:956	Moreover, PEMs obtained from GS-P-treated mice showed significantly higher tumoricidal activity against colon 26-M3.1 cells, and splenocytes from GS-P-treated mice significantly enhanced NK cell cytotoxicity against YAC-1 tumor cells.
28522391	3	44	theme	dose-dependent	462:475	arg1	manner					477:482	a dose-dependent manner	460:482	a dose-dependent manner	460:482	GS-P treatment significantly inhibited metastasis in mice, in a dose-dependent manner.
28522391	0	45	dep	ginseng	21:27	arg1	leaves					29:34	leaves	29:34	leaves	29:34	Polysaccharides from ginseng leaves inhibit tumor metastasis via macrophage and NK cell activation.
28522391	1	46	theme	polysaccharide	131:144	arg1	GS-P					156:159	GS-P	156:159	GS-P	156:159	In this study, we purified the polysaccharide fraction (GS-P) from the leaves of Panax ginseng C.A. Meyer and analyzed its monosaccharide composition and antitumor and antimetastatic activity in vitro and in vivo.
28522391	1	46	theme	polysaccharide	131:144	arg1	fraction					146:153	the polysaccharide fraction	127:153	the polysaccharide fraction (GS-P)	127:160	In this study, we purified the polysaccharide fraction (GS-P) from the leaves of Panax ginseng C.A. Meyer and analyzed its monosaccharide composition and antitumor and antimetastatic activity in vitro and in vivo.
28522391	5	47	theme	mice	718:721	arg1	PEMs					696:699	PEMs	696:699	PEMs	696:699	Secretion of tumor necrosis factor (TNF)-α and interleukin (IL)-12 was enhanced in the peritoneal exudate macrophages (PEMs) of GS-P-treated mice.
28522391	5	47	theme	mice	718:721	arg1	macrophages					683:693	the peritoneal exudate macrophages	660:693	the peritoneal exudate macrophages (PEMs) of GS-P-treated mice	660:721	Secretion of tumor necrosis factor (TNF)-α and interleukin (IL)-12 was enhanced in the peritoneal exudate macrophages (PEMs) of GS-P-treated mice.
28522391	6	48	theme	tumoricidal	799:809	arg1	activity					811:818	significantly higher tumoricidal activity	778:818	significantly higher tumoricidal activity against colon 26-M3.1 cells	778:846	Moreover, PEMs obtained from GS-P-treated mice showed significantly higher tumoricidal activity against colon 26-M3.1 cells, and splenocytes from GS-P-treated mice significantly enhanced NK cell cytotoxicity against YAC-1 tumor cells.
28522391	0	49	theme	macrophage	65:74	arg1	activation					88:97	macrophage and NK cell activation	65:97	activation	88:97	Polysaccharides from ginseng leaves inhibit tumor metastasis via macrophage and NK cell activation.
28522391	6	50	theme	cell	914:917	arg1	cytotoxicity					919:930	NK cell cytotoxicity	911:930	NK cell cytotoxicity	911:930	Moreover, PEMs obtained from GS-P-treated mice showed significantly higher tumoricidal activity against colon 26-M3.1 cells, and splenocytes from GS-P-treated mice significantly enhanced NK cell cytotoxicity against YAC-1 tumor cells.
28522391	7	51	theme	anti-asialo	977:987	arg1	GM1					989:991	anti-asialo GM1	977:991	anti-asialo GM1 (an antibody for NK cell depletion)	977:1027	Pretreatment with anti-asialo GM1 (an antibody for NK cell depletion) partly suppressed the inhibitory effects of GS-P on lung metastasis.
28522391	2	52	theme	pectic	332:337	arg1	GS-P					314:317	GS-P	314:317	GS-P	314:317	GS-P is a 10.2kDa pectic polysaccharide consisting of 15 different monosaccharides.
28522391	2	52	theme	pectic	332:337	arg1	polysaccharide					339:352	a 10.2kDa pectic polysaccharide	322:352	a 10.2kDa pectic polysaccharide consisting of 15 different monosaccharides	322:395	GS-P is a 10.2kDa pectic polysaccharide consisting of 15 different monosaccharides.
28522391	6	53	theme	YAC-1	940:944	arg1	cells					952:956	YAC-1 tumor cells	940:956	YAC-1 tumor cells	940:956	Moreover, PEMs obtained from GS-P-treated mice showed significantly higher tumoricidal activity against colon 26-M3.1 cells, and splenocytes from GS-P-treated mice significantly enhanced NK cell cytotoxicity against YAC-1 tumor cells.
25955786	2	0	theme	mixtures	460:467	arg1	dynamics					418:425	the fast dynamics	409:425	the fast dynamics of dehydrated trehalose/glycerol mixtures	409:467	This finding inspired great interest in the fast dynamics of dehydrated trehalose/glycerol mixtures, leading to the observation that suppression of fast dynamics was optimal in the presence of ∼5 wt % of glycerol.
25955786	1	1	theme	glass	313:317	arg1	transition					319:328	the final glass transition	303:328	the final glass transition temperature (Tg)	303:345	Approximately a decade ago it was observed that adding a small amount (5 wt %) of glycerol to trehalose could substantially improve the stability of enzymes stored in these glasses even though the final glass transition temperature (Tg) was reduced by ∼20 K.
25955786	2	2	theme	wt	565:566	arg1	glycerol					573:580	glycerol	573:580	glycerol	573:580	This finding inspired great interest in the fast dynamics of dehydrated trehalose/glycerol mixtures, leading to the observation that suppression of fast dynamics was optimal in the presence of ∼5 wt % of glycerol.
25955786	2	2	theme	wt	565:566	arg1	%					568:568	∼5 wt %	562:568	∼5 wt % of glycerol	562:580	This finding inspired great interest in the fast dynamics of dehydrated trehalose/glycerol mixtures, leading to the observation that suppression of fast dynamics was optimal in the presence of ∼5 wt % of glycerol.
25955786	4	3	theme	trehalose	1043:1051	arg1	trehalose					1043:1051	trehalose	1043:1051	trehalose	1043:1051	In the present study a dynamic mechanical analyzer (DMA) was used to determine both the Tg and the kinetic fragility index (m) of trehalose/glycerol mixtures within the mass fraction range of 80-100 wt % of trehalose.
25955786	4	3	theme	trehalose	1043:1051	arg1	%					1038:1038	80-100 wt %	1028:1038	80-100 wt % of trehalose	1028:1051	In the present study a dynamic mechanical analyzer (DMA) was used to determine both the Tg and the kinetic fragility index (m) of trehalose/glycerol mixtures within the mass fraction range of 80-100 wt % of trehalose.
25955786	8	4	theme	DMA-based	1675:1683	arg1	Tg					1685:1686	The DMA-based Tg and fragility determination method	1671:1721	Tg	1685:1686	The DMA-based Tg and fragility determination method developed in this study represents a new approach for identifying optimal compositions for preservation of biologics.
25955786	4	5	theme	fragility	943:951	arg1	m					960:960	m	960:960	m	960:960	In the present study a dynamic mechanical analyzer (DMA) was used to determine both the Tg and the kinetic fragility index (m) of trehalose/glycerol mixtures within the mass fraction range of 80-100 wt % of trehalose.
25955786	4	5	theme	fragility	943:951	arg1	index					953:957	the kinetic fragility index	931:957	the kinetic fragility index (m)	931:961	In the present study a dynamic mechanical analyzer (DMA) was used to determine both the Tg and the kinetic fragility index (m) of trehalose/glycerol mixtures within the mass fraction range of 80-100 wt % of trehalose.
25955786	2	6	theme	trehalose/glycerol	441:458	arg1	mixtures					460:467	dehydrated trehalose/glycerol mixtures	430:467	dehydrated trehalose/glycerol mixtures	430:467	This finding inspired great interest in the fast dynamics of dehydrated trehalose/glycerol mixtures, leading to the observation that suppression of fast dynamics was optimal in the presence of ∼5 wt % of glycerol.
25955786	0	7	from	minimum	6:12	arg1	fragility					17:25	fragility	17:25	fragility	17:25	Local minimum in fragility for trehalose/glycerol mixtures: implications for biopharmaceutical stabilization.
25955786	6	8	theme	lowering	1509:1516	arg1	effect					1518:1523	its Tg lowering effect	1502:1523	its Tg lowering effect	1502:1523	The composition of 5-12.5 wt % glycerol in trehalose thus yielded a matrix that maximized the strong glass-forming contribution of glycerol, while minimizing its Tg lowering effect.
25955786	7	9	theme	past	1658:1661	arg1	decade					1663:1668	the past decade	1654:1668	the past decade	1654:1668	This quantitative evidence supports speculation about the fragility characteristics of these mixtures that has been ongoing for the past decade.
25955786	1	10	theme	small	167:171	arg1	amount					173:178	a small amount	165:178	a small amount (5 wt %) of glycerol	165:199	Approximately a decade ago it was observed that adding a small amount (5 wt %) of glycerol to trehalose could substantially improve the stability of enzymes stored in these glasses even though the final glass transition temperature (Tg) was reduced by ∼20 K.
25955786	1	10	theme	small	167:171	arg1	%					186:186	5 wt %	181:186	5 wt %	181:186	Approximately a decade ago it was observed that adding a small amount (5 wt %) of glycerol to trehalose could substantially improve the stability of enzymes stored in these glasses even though the final glass transition temperature (Tg) was reduced by ∼20 K.
25955786	1	10	theme	small	167:171	arg1	glycerol					192:199	glycerol	192:199	glycerol	192:199	Approximately a decade ago it was observed that adding a small amount (5 wt %) of glycerol to trehalose could substantially improve the stability of enzymes stored in these glasses even though the final glass transition temperature (Tg) was reduced by ∼20 K.
25955786	5	11	theme	fragility	1076:1084	arg1	index					1086:1090	the fragility index	1072:1090	the fragility index	1072:1090	It was found that the fragility index correlated with the mass fraction of trehalose in a nonmonotonic manner, with a local minimum between 87.5 and 95 wt % of trehalose, whereas the composition dependence of Tg was found to follow a Gordon-Taylor-like relationship, with no local minimum.
25955786	4	12	theme	mechanical	867:876	arg1	analyzer					878:885	a dynamic mechanical analyzer	857:885	a dynamic mechanical analyzer (DMA)	857:891	In the present study a dynamic mechanical analyzer (DMA) was used to determine both the Tg and the kinetic fragility index (m) of trehalose/glycerol mixtures within the mass fraction range of 80-100 wt % of trehalose.
25955786	4	12	theme	mechanical	867:876	arg1	DMA					888:890	DMA	888:890	DMA	888:890	In the present study a dynamic mechanical analyzer (DMA) was used to determine both the Tg and the kinetic fragility index (m) of trehalose/glycerol mixtures within the mass fraction range of 80-100 wt % of trehalose.
25955786	2	13	theme	dehydrated	430:439	arg1	mixtures					460:467	dehydrated trehalose/glycerol mixtures	430:467	dehydrated trehalose/glycerol mixtures	430:467	This finding inspired great interest in the fast dynamics of dehydrated trehalose/glycerol mixtures, leading to the observation that suppression of fast dynamics was optimal in the presence of ∼5 wt % of glycerol.
25955786	3	14	theme	other	805:809	arg1	mixtures					811:818	any other mixtures	801:818	any other mixtures of this nature	801:833	It was also recognized that the fast dynamics should, in theory, be related to the fragility of these glass formers, but experimental confirmation of this hypothesis has been lacking for trehalose/glycerol mixtures or any other mixtures of this nature.
25955786	3	15	theme	hypothesis	738:747	arg1	confirmation					717:728	experimental confirmation	704:728	experimental confirmation of this hypothesis	704:747	It was also recognized that the fast dynamics should, in theory, be related to the fragility of these glass formers, but experimental confirmation of this hypothesis has been lacking for trehalose/glycerol mixtures or any other mixtures of this nature.
25955786	4	16	theme	dynamic	859:865	arg1	analyzer					878:885	a dynamic mechanical analyzer	857:885	a dynamic mechanical analyzer (DMA)	857:891	In the present study a dynamic mechanical analyzer (DMA) was used to determine both the Tg and the kinetic fragility index (m) of trehalose/glycerol mixtures within the mass fraction range of 80-100 wt % of trehalose.
25955786	4	16	theme	dynamic	859:865	arg1	DMA					888:890	DMA	888:890	DMA	888:890	In the present study a dynamic mechanical analyzer (DMA) was used to determine both the Tg and the kinetic fragility index (m) of trehalose/glycerol mixtures within the mass fraction range of 80-100 wt % of trehalose.
25955786	6	17	theme	glycerol	1375:1382	arg1	composition					1348:1358	The composition	1344:1358	The composition of 5-12.5 wt % glycerol in trehalose	1344:1395	The composition of 5-12.5 wt % glycerol in trehalose thus yielded a matrix that maximized the strong glass-forming contribution of glycerol, while minimizing its Tg lowering effect.
25955786	5	18	theme	87.5	1194:1197	arg1	trehalose					1214:1222	trehalose	1214:1222	trehalose	1214:1222	It was found that the fragility index correlated with the mass fraction of trehalose in a nonmonotonic manner, with a local minimum between 87.5 and 95 wt % of trehalose, whereas the composition dependence of Tg was found to follow a Gordon-Taylor-like relationship, with no local minimum.
25955786	5	18	theme	87.5	1194:1197	arg1	%					1209:1209	87.5 and 95 wt %	1194:1209	%	1209:1209	It was found that the fragility index correlated with the mass fraction of trehalose in a nonmonotonic manner, with a local minimum between 87.5 and 95 wt % of trehalose, whereas the composition dependence of Tg was found to follow a Gordon-Taylor-like relationship, with no local minimum.
25955786	5	19	theme	wt	1206:1207	arg1	trehalose					1214:1222	trehalose	1214:1222	trehalose	1214:1222	It was found that the fragility index correlated with the mass fraction of trehalose in a nonmonotonic manner, with a local minimum between 87.5 and 95 wt % of trehalose, whereas the composition dependence of Tg was found to follow a Gordon-Taylor-like relationship, with no local minimum.
25955786	5	19	theme	wt	1206:1207	arg1	%					1209:1209	87.5 and 95 wt %	1194:1209	%	1209:1209	It was found that the fragility index correlated with the mass fraction of trehalose in a nonmonotonic manner, with a local minimum between 87.5 and 95 wt % of trehalose, whereas the composition dependence of Tg was found to follow a Gordon-Taylor-like relationship, with no local minimum.
25955786	5	20	from	manner	1157:1162	arg1	fraction					1117:1124	the mass fraction	1108:1124	the mass fraction of trehalose in a nonmonotonic manner	1108:1162	It was found that the fragility index correlated with the mass fraction of trehalose in a nonmonotonic manner, with a local minimum between 87.5 and 95 wt % of trehalose, whereas the composition dependence of Tg was found to follow a Gordon-Taylor-like relationship, with no local minimum.
25955786	5	21	from	fraction	1117:1124	arg1	manner					1157:1162	a nonmonotonic manner	1142:1162	a nonmonotonic manner	1142:1162	It was found that the fragility index correlated with the mass fraction of trehalose in a nonmonotonic manner, with a local minimum between 87.5 and 95 wt % of trehalose, whereas the composition dependence of Tg was found to follow a Gordon-Taylor-like relationship, with no local minimum.
25955786	5	22	theme	trehalose	1129:1137	arg1	fraction					1117:1124	the mass fraction	1108:1124	the mass fraction of trehalose in a nonmonotonic manner	1108:1162	It was found that the fragility index correlated with the mass fraction of trehalose in a nonmonotonic manner, with a local minimum between 87.5 and 95 wt % of trehalose, whereas the composition dependence of Tg was found to follow a Gordon-Taylor-like relationship, with no local minimum.
25955786	8	23	theme	new	1760:1762	arg1	approach					1764:1771	a new approach	1758:1771	a new approach for identifying optimal compositions for preservation of biologics	1758:1838	The DMA-based Tg and fragility determination method developed in this study represents a new approach for identifying optimal compositions for preservation of biologics.
25955786	4	24	dep	Tg	924:925	arg1	the					920:922	the	920:922	the	920:922	In the present study a dynamic mechanical analyzer (DMA) was used to determine both the Tg and the kinetic fragility index (m) of trehalose/glycerol mixtures within the mass fraction range of 80-100 wt % of trehalose.
25955786	3	25	from	theory	640:645	arg1	related					651:657	related	651:657	related	651:657	It was also recognized that the fast dynamics should, in theory, be related to the fragility of these glass formers, but experimental confirmation of this hypothesis has been lacking for trehalose/glycerol mixtures or any other mixtures of this nature.
25955786	3	25	from	theory	640:645	arg1	dynamics					620:627	the fast dynamics	611:627	the fast dynamics	611:627	It was also recognized that the fast dynamics should, in theory, be related to the fragility of these glass formers, but experimental confirmation of this hypothesis has been lacking for trehalose/glycerol mixtures or any other mixtures of this nature.
25955786	2	26	theme	glycerol	573:580	arg1	glycerol					573:580	glycerol	573:580	glycerol	573:580	This finding inspired great interest in the fast dynamics of dehydrated trehalose/glycerol mixtures, leading to the observation that suppression of fast dynamics was optimal in the presence of ∼5 wt % of glycerol.
25955786	2	26	theme	glycerol	573:580	arg1	%					568:568	∼5 wt %	562:568	∼5 wt % of glycerol	562:580	This finding inspired great interest in the fast dynamics of dehydrated trehalose/glycerol mixtures, leading to the observation that suppression of fast dynamics was optimal in the presence of ∼5 wt % of glycerol.
25955786	6	27	theme	glycerol	1475:1482	arg1	contribution					1459:1470	the strong glass-forming contribution	1434:1470	the strong glass-forming contribution of glycerol	1434:1482	The composition of 5-12.5 wt % glycerol in trehalose thus yielded a matrix that maximized the strong glass-forming contribution of glycerol, while minimizing its Tg lowering effect.
25955786	0	28	theme	Local	0:4	arg1	minimum					6:12	Local minimum	0:12	Local minimum in fragility for trehalose/glycerol mixtures: implications for biopharmaceutical stabilization.	0:108	Local minimum in fragility for trehalose/glycerol mixtures: implications for biopharmaceutical stabilization.
25955786	4	29	theme	wt	1035:1036	arg1	trehalose					1043:1051	trehalose	1043:1051	trehalose	1043:1051	In the present study a dynamic mechanical analyzer (DMA) was used to determine both the Tg and the kinetic fragility index (m) of trehalose/glycerol mixtures within the mass fraction range of 80-100 wt % of trehalose.
25955786	4	29	theme	wt	1035:1036	arg1	%					1038:1038	80-100 wt %	1028:1038	80-100 wt % of trehalose	1028:1051	In the present study a dynamic mechanical analyzer (DMA) was used to determine both the Tg and the kinetic fragility index (m) of trehalose/glycerol mixtures within the mass fraction range of 80-100 wt % of trehalose.
25955786	0	30	theme	biopharmaceutical	77:93	arg1	stabilization					95:107	biopharmaceutical stabilization	77:107	biopharmaceutical stabilization	77:107	Local minimum in fragility for trehalose/glycerol mixtures: implications for biopharmaceutical stabilization.
25955786	6	31	theme	Tg	1506:1507	arg1	effect					1518:1523	its Tg lowering effect	1502:1523	its Tg lowering effect	1502:1523	The composition of 5-12.5 wt % glycerol in trehalose thus yielded a matrix that maximized the strong glass-forming contribution of glycerol, while minimizing its Tg lowering effect.
25955786	5	32	theme	Tg	1263:1264	arg1	dependence					1249:1258	the composition dependence	1233:1258	the composition dependence of Tg	1233:1264	It was found that the fragility index correlated with the mass fraction of trehalose in a nonmonotonic manner, with a local minimum between 87.5 and 95 wt % of trehalose, whereas the composition dependence of Tg was found to follow a Gordon-Taylor-like relationship, with no local minimum.
25955786	3	33	theme	nature	828:833	arg1	mixtures					789:796	trehalose/glycerol mixtures	770:796	trehalose/glycerol mixtures	770:796	It was also recognized that the fast dynamics should, in theory, be related to the fragility of these glass formers, but experimental confirmation of this hypothesis has been lacking for trehalose/glycerol mixtures or any other mixtures of this nature.
25955786	3	33	theme	nature	828:833	arg1	mixtures					811:818	any other mixtures	801:818	any other mixtures of this nature	801:833	It was also recognized that the fast dynamics should, in theory, be related to the fragility of these glass formers, but experimental confirmation of this hypothesis has been lacking for trehalose/glycerol mixtures or any other mixtures of this nature.
25955786	6	34	theme	%	1373:1373	arg1	glycerol					1375:1382	5-12.5 wt % glycerol	1363:1382	5-12.5 wt % glycerol	1363:1382	The composition of 5-12.5 wt % glycerol in trehalose thus yielded a matrix that maximized the strong glass-forming contribution of glycerol, while minimizing its Tg lowering effect.
25955786	1	35	theme	transition	319:328	arg1	Tg					343:344	Tg	343:344	Tg	343:344	Approximately a decade ago it was observed that adding a small amount (5 wt %) of glycerol to trehalose could substantially improve the stability of enzymes stored in these glasses even though the final glass transition temperature (Tg) was reduced by ∼20 K.
25955786	1	35	theme	transition	319:328	arg1	temperature					330:340	the final glass transition temperature	303:340	the final glass transition temperature (Tg)	303:345	Approximately a decade ago it was observed that adding a small amount (5 wt %) of glycerol to trehalose could substantially improve the stability of enzymes stored in these glasses even though the final glass transition temperature (Tg) was reduced by ∼20 K.
25955786	1	36	theme	enzymes	259:265	arg1	stability					246:254	the stability	242:254	the stability of enzymes stored in these glasses	242:289	Approximately a decade ago it was observed that adding a small amount (5 wt %) of glycerol to trehalose could substantially improve the stability of enzymes stored in these glasses even though the final glass transition temperature (Tg) was reduced by ∼20 K.
25955786	2	37	theme	%	568:568	arg1	optimal					535:541	optimal	535:541	optimal	535:541	This finding inspired great interest in the fast dynamics of dehydrated trehalose/glycerol mixtures, leading to the observation that suppression of fast dynamics was optimal in the presence of ∼5 wt % of glycerol.
25955786	6	38	from	composition	1348:1358	arg1	trehalose					1387:1395	trehalose	1387:1395	trehalose	1387:1395	The composition of 5-12.5 wt % glycerol in trehalose thus yielded a matrix that maximized the strong glass-forming contribution of glycerol, while minimizing its Tg lowering effect.
25955786	6	39	theme	wt	1370:1371	arg1	glycerol					1375:1382	5-12.5 wt % glycerol	1363:1382	5-12.5 wt % glycerol	1363:1382	The composition of 5-12.5 wt % glycerol in trehalose thus yielded a matrix that maximized the strong glass-forming contribution of glycerol, while minimizing its Tg lowering effect.
25955786	1	40	theme	wt	183:184	arg1	amount					173:178	a small amount	165:178	a small amount (5 wt %) of glycerol	165:199	Approximately a decade ago it was observed that adding a small amount (5 wt %) of glycerol to trehalose could substantially improve the stability of enzymes stored in these glasses even though the final glass transition temperature (Tg) was reduced by ∼20 K.
25955786	1	40	theme	wt	183:184	arg1	%					186:186	5 wt %	181:186	5 wt %	181:186	Approximately a decade ago it was observed that adding a small amount (5 wt %) of glycerol to trehalose could substantially improve the stability of enzymes stored in these glasses even though the final glass transition temperature (Tg) was reduced by ∼20 K.
25955786	1	40	theme	wt	183:184	arg1	glycerol					192:199	glycerol	192:199	glycerol	192:199	Approximately a decade ago it was observed that adding a small amount (5 wt %) of glycerol to trehalose could substantially improve the stability of enzymes stored in these glasses even though the final glass transition temperature (Tg) was reduced by ∼20 K.
25955786	3	41	from	related	651:657	arg1	theory					640:645	theory	640:645	theory	640:645	It was also recognized that the fast dynamics should, in theory, be related to the fragility of these glass formers, but experimental confirmation of this hypothesis has been lacking for trehalose/glycerol mixtures or any other mixtures of this nature.
25955786	2	42	theme	dynamics	522:529	arg1	suppression					502:512	suppression	502:512	suppression of fast dynamics	502:529	This finding inspired great interest in the fast dynamics of dehydrated trehalose/glycerol mixtures, leading to the observation that suppression of fast dynamics was optimal in the presence of ∼5 wt % of glycerol.
25955786	5	43	theme	Gordon-Taylor-like	1288:1305	arg1	relationship					1307:1318	a Gordon-Taylor-like relationship	1286:1318	a Gordon-Taylor-like relationship	1286:1318	It was found that the fragility index correlated with the mass fraction of trehalose in a nonmonotonic manner, with a local minimum between 87.5 and 95 wt % of trehalose, whereas the composition dependence of Tg was found to follow a Gordon-Taylor-like relationship, with no local minimum.
25955786	5	44	theme	local	1172:1176	arg1	minimum					1178:1184	a local minimum	1170:1184	a local minimum between 87.5 and 95 wt % of trehalose	1170:1222	It was found that the fragility index correlated with the mass fraction of trehalose in a nonmonotonic manner, with a local minimum between 87.5 and 95 wt % of trehalose, whereas the composition dependence of Tg was found to follow a Gordon-Taylor-like relationship, with no local minimum.
25955786	2	45	dep	%	568:568	arg1	presence					550:557	presence	550:557	presence	550:557	This finding inspired great interest in the fast dynamics of dehydrated trehalose/glycerol mixtures, leading to the observation that suppression of fast dynamics was optimal in the presence of ∼5 wt % of glycerol.
25955786	2	45	dep	%	568:568	arg1	the					546:548	the	546:548	the	546:548	This finding inspired great interest in the fast dynamics of dehydrated trehalose/glycerol mixtures, leading to the observation that suppression of fast dynamics was optimal in the presence of ∼5 wt % of glycerol.
25955786	2	46	theme	great	391:395	arg1	interest					397:404	great interest	391:404	great interest in the fast dynamics of dehydrated trehalose/glycerol mixtures	391:467	This finding inspired great interest in the fast dynamics of dehydrated trehalose/glycerol mixtures, leading to the observation that suppression of fast dynamics was optimal in the presence of ∼5 wt % of glycerol.
25955786	2	47	theme	fast	517:520	arg1	dynamics					522:529	fast dynamics	517:529	fast dynamics	517:529	This finding inspired great interest in the fast dynamics of dehydrated trehalose/glycerol mixtures, leading to the observation that suppression of fast dynamics was optimal in the presence of ∼5 wt % of glycerol.
25955786	4	48	theme	mass	1005:1008	arg1	range					1019:1023	the mass fraction range	1001:1023	the mass fraction range of 80-100 wt % of trehalose	1001:1051	In the present study a dynamic mechanical analyzer (DMA) was used to determine both the Tg and the kinetic fragility index (m) of trehalose/glycerol mixtures within the mass fraction range of 80-100 wt % of trehalose.
25955786	4	49	theme	fraction	1010:1017	arg1	range					1019:1023	the mass fraction range	1001:1023	the mass fraction range of 80-100 wt % of trehalose	1001:1051	In the present study a dynamic mechanical analyzer (DMA) was used to determine both the Tg and the kinetic fragility index (m) of trehalose/glycerol mixtures within the mass fraction range of 80-100 wt % of trehalose.
25955786	0	50	theme	trehalose/glycerol	31:48	arg1	mixtures					50:57	trehalose/glycerol mixtures	31:57	trehalose/glycerol mixtures	31:57	Local minimum in fragility for trehalose/glycerol mixtures: implications for biopharmaceutical stabilization.
25955786	4	51	used	used	897:900	arg2	DMA					888:890	DMA	888:890	DMA	888:890	In the present study a dynamic mechanical analyzer (DMA) was used to determine both the Tg and the kinetic fragility index (m) of trehalose/glycerol mixtures within the mass fraction range of 80-100 wt % of trehalose.
25955786	4	51	used	used	897:900	arg2	analyzer					878:885	a dynamic mechanical analyzer	857:885	a dynamic mechanical analyzer (DMA)	857:891	In the present study a dynamic mechanical analyzer (DMA) was used to determine both the Tg and the kinetic fragility index (m) of trehalose/glycerol mixtures within the mass fraction range of 80-100 wt % of trehalose.
25955786	4	52	theme	trehalose/glycerol	966:983	arg1	mixtures					985:992	trehalose/glycerol mixtures	966:992	trehalose/glycerol mixtures within the mass fraction range of 80-100 wt % of trehalose	966:1051	In the present study a dynamic mechanical analyzer (DMA) was used to determine both the Tg and the kinetic fragility index (m) of trehalose/glycerol mixtures within the mass fraction range of 80-100 wt % of trehalose.
25955786	8	53	theme	optimal	1789:1795	arg1	compositions					1797:1808	optimal compositions	1789:1808	optimal compositions for preservation of biologics	1789:1838	The DMA-based Tg and fragility determination method developed in this study represents a new approach for identifying optimal compositions for preservation of biologics.
25955786	4	54	theme	kinetic	935:941	arg1	m					960:960	m	960:960	m	960:960	In the present study a dynamic mechanical analyzer (DMA) was used to determine both the Tg and the kinetic fragility index (m) of trehalose/glycerol mixtures within the mass fraction range of 80-100 wt % of trehalose.
25955786	4	54	theme	kinetic	935:941	arg1	index					953:957	the kinetic fragility index	931:957	the kinetic fragility index (m)	931:961	In the present study a dynamic mechanical analyzer (DMA) was used to determine both the Tg and the kinetic fragility index (m) of trehalose/glycerol mixtures within the mass fraction range of 80-100 wt % of trehalose.
25955786	6	55	theme	strong	1438:1443	arg1	contribution					1459:1470	the strong glass-forming contribution	1434:1470	the strong glass-forming contribution of glycerol	1434:1482	The composition of 5-12.5 wt % glycerol in trehalose thus yielded a matrix that maximized the strong glass-forming contribution of glycerol, while minimizing its Tg lowering effect.
25955786	1	56	theme	glycerol	192:199	arg1	amount					173:178	a small amount	165:178	a small amount (5 wt %) of glycerol	165:199	Approximately a decade ago it was observed that adding a small amount (5 wt %) of glycerol to trehalose could substantially improve the stability of enzymes stored in these glasses even though the final glass transition temperature (Tg) was reduced by ∼20 K.
25955786	1	56	theme	glycerol	192:199	arg1	%					186:186	5 wt %	181:186	5 wt %	181:186	Approximately a decade ago it was observed that adding a small amount (5 wt %) of glycerol to trehalose could substantially improve the stability of enzymes stored in these glasses even though the final glass transition temperature (Tg) was reduced by ∼20 K.
25955786	1	56	theme	glycerol	192:199	arg1	glycerol					192:199	glycerol	192:199	glycerol	192:199	Approximately a decade ago it was observed that adding a small amount (5 wt %) of glycerol to trehalose could substantially improve the stability of enzymes stored in these glasses even though the final glass transition temperature (Tg) was reduced by ∼20 K.
25955786	5	57	theme	local	1329:1333	arg1	minimum					1335:1341	no local minimum	1326:1341	no local minimum	1326:1341	It was found that the fragility index correlated with the mass fraction of trehalose in a nonmonotonic manner, with a local minimum between 87.5 and 95 wt % of trehalose, whereas the composition dependence of Tg was found to follow a Gordon-Taylor-like relationship, with no local minimum.
25955786	2	58	theme	fast	413:416	arg1	dynamics					418:425	the fast dynamics	409:425	the fast dynamics of dehydrated trehalose/glycerol mixtures	409:467	This finding inspired great interest in the fast dynamics of dehydrated trehalose/glycerol mixtures, leading to the observation that suppression of fast dynamics was optimal in the presence of ∼5 wt % of glycerol.
25955786	3	59	theme	glass	685:689	arg1	formers					691:697	these glass formers	679:697	these glass formers	679:697	It was also recognized that the fast dynamics should, in theory, be related to the fragility of these glass formers, but experimental confirmation of this hypothesis has been lacking for trehalose/glycerol mixtures or any other mixtures of this nature.
25955786	2	60	from	interest	397:404	arg1	dynamics					418:425	the fast dynamics	409:425	the fast dynamics of dehydrated trehalose/glycerol mixtures	409:467	This finding inspired great interest in the fast dynamics of dehydrated trehalose/glycerol mixtures, leading to the observation that suppression of fast dynamics was optimal in the presence of ∼5 wt % of glycerol.
25955786	3	61	theme	fast	615:618	arg1	related					651:657	related	651:657	related	651:657	It was also recognized that the fast dynamics should, in theory, be related to the fragility of these glass formers, but experimental confirmation of this hypothesis has been lacking for trehalose/glycerol mixtures or any other mixtures of this nature.
25955786	3	61	theme	fast	615:618	arg1	dynamics					620:627	the fast dynamics	611:627	the fast dynamics	611:627	It was also recognized that the fast dynamics should, in theory, be related to the fragility of these glass formers, but experimental confirmation of this hypothesis has been lacking for trehalose/glycerol mixtures or any other mixtures of this nature.
25955786	7	62	theme	quantitative	1531:1542	arg1	evidence					1544:1551	This quantitative evidence	1526:1551	This quantitative evidence	1526:1551	This quantitative evidence supports speculation about the fragility characteristics of these mixtures that has been ongoing for the past decade.
25955786	5	63	theme	composition	1237:1247	arg1	dependence					1249:1258	the composition dependence	1233:1258	the composition dependence of Tg	1233:1264	It was found that the fragility index correlated with the mass fraction of trehalose in a nonmonotonic manner, with a local minimum between 87.5 and 95 wt % of trehalose, whereas the composition dependence of Tg was found to follow a Gordon-Taylor-like relationship, with no local minimum.
25955786	5	64	theme	nonmonotonic	1144:1155	arg1	manner					1157:1162	a nonmonotonic manner	1142:1162	a nonmonotonic manner	1142:1162	It was found that the fragility index correlated with the mass fraction of trehalose in a nonmonotonic manner, with a local minimum between 87.5 and 95 wt % of trehalose, whereas the composition dependence of Tg was found to follow a Gordon-Taylor-like relationship, with no local minimum.
25955786	3	65	theme	formers	691:697	arg1	fragility					666:674	the fragility	662:674	the fragility of these glass formers	662:697	It was also recognized that the fast dynamics should, in theory, be related to the fragility of these glass formers, but experimental confirmation of this hypothesis has been lacking for trehalose/glycerol mixtures or any other mixtures of this nature.
25955786	8	66	theme	biologics	1830:1838	arg1	preservation					1814:1825	preservation	1814:1825	preservation of biologics	1814:1838	The DMA-based Tg and fragility determination method developed in this study represents a new approach for identifying optimal compositions for preservation of biologics.
25955786	8	67	theme	fragility	1692:1700	arg1	method					1716:1721	The DMA-based Tg and fragility determination method	1671:1721	method	1716:1721	The DMA-based Tg and fragility determination method developed in this study represents a new approach for identifying optimal compositions for preservation of biologics.
25955786	7	68	theme	fragility	1584:1592	arg1	characteristics					1594:1608	the fragility characteristics	1580:1608	the fragility characteristics of these mixtures that has been ongoing for the past decade	1580:1668	This quantitative evidence supports speculation about the fragility characteristics of these mixtures that has been ongoing for the past decade.
25955786	7	69	theme	mixtures	1619:1626	arg1	characteristics					1594:1608	the fragility characteristics	1580:1608	the fragility characteristics of these mixtures that has been ongoing for the past decade	1580:1668	This quantitative evidence supports speculation about the fragility characteristics of these mixtures that has been ongoing for the past decade.
25955786	5	70	from	trehalose	1129:1137	arg1	manner					1157:1162	a nonmonotonic manner	1142:1162	a nonmonotonic manner	1142:1162	It was found that the fragility index correlated with the mass fraction of trehalose in a nonmonotonic manner, with a local minimum between 87.5 and 95 wt % of trehalose, whereas the composition dependence of Tg was found to follow a Gordon-Taylor-like relationship, with no local minimum.
25955786	6	71	theme	glass-forming	1445:1457	arg1	contribution					1459:1470	the strong glass-forming contribution	1434:1470	the strong glass-forming contribution of glycerol	1434:1482	The composition of 5-12.5 wt % glycerol in trehalose thus yielded a matrix that maximized the strong glass-forming contribution of glycerol, while minimizing its Tg lowering effect.
25955786	8	72	theme	determination	1702:1714	arg1	method					1716:1721	The DMA-based Tg and fragility determination method	1671:1721	method	1716:1721	The DMA-based Tg and fragility determination method developed in this study represents a new approach for identifying optimal compositions for preservation of biologics.
25955786	4	73	theme	mixtures	985:992	arg1	m					960:960	m	960:960	m	960:960	In the present study a dynamic mechanical analyzer (DMA) was used to determine both the Tg and the kinetic fragility index (m) of trehalose/glycerol mixtures within the mass fraction range of 80-100 wt % of trehalose.
25955786	4	73	theme	mixtures	985:992	arg1	index					953:957	the kinetic fragility index	931:957	the kinetic fragility index (m)	931:961	In the present study a dynamic mechanical analyzer (DMA) was used to determine both the Tg and the kinetic fragility index (m) of trehalose/glycerol mixtures within the mass fraction range of 80-100 wt % of trehalose.
25955786	4	73	theme	mixtures	985:992	arg1	Tg					924:925	Tg	924:925	Tg	924:925	In the present study a dynamic mechanical analyzer (DMA) was used to determine both the Tg and the kinetic fragility index (m) of trehalose/glycerol mixtures within the mass fraction range of 80-100 wt % of trehalose.
25955786	5	74	theme	95	1203:1204	arg1	trehalose					1214:1222	trehalose	1214:1222	trehalose	1214:1222	It was found that the fragility index correlated with the mass fraction of trehalose in a nonmonotonic manner, with a local minimum between 87.5 and 95 wt % of trehalose, whereas the composition dependence of Tg was found to follow a Gordon-Taylor-like relationship, with no local minimum.
25955786	5	74	theme	95	1203:1204	arg1	%					1209:1209	87.5 and 95 wt %	1194:1209	%	1209:1209	It was found that the fragility index correlated with the mass fraction of trehalose in a nonmonotonic manner, with a local minimum between 87.5 and 95 wt % of trehalose, whereas the composition dependence of Tg was found to follow a Gordon-Taylor-like relationship, with no local minimum.
25955786	4	75	theme	present	843:849	arg1	study					851:855	the present study	839:855	the present study	839:855	In the present study a dynamic mechanical analyzer (DMA) was used to determine both the Tg and the kinetic fragility index (m) of trehalose/glycerol mixtures within the mass fraction range of 80-100 wt % of trehalose.
25955786	0	76	dep	minimum	6:12	arg1	implications					60:71	implications	60:71	Local minimum in fragility for trehalose/glycerol mixtures: implications for biopharmaceutical stabilization.	0:108	Local minimum in fragility for trehalose/glycerol mixtures: implications for biopharmaceutical stabilization.
25955786	4	77	theme	%	1038:1038	arg1	range					1019:1023	the mass fraction range	1001:1023	the mass fraction range of 80-100 wt % of trehalose	1001:1051	In the present study a dynamic mechanical analyzer (DMA) was used to determine both the Tg and the kinetic fragility index (m) of trehalose/glycerol mixtures within the mass fraction range of 80-100 wt % of trehalose.
25955786	3	78	theme	trehalose/glycerol	770:787	arg1	mixtures					789:796	trehalose/glycerol mixtures	770:796	trehalose/glycerol mixtures	770:796	It was also recognized that the fast dynamics should, in theory, be related to the fragility of these glass formers, but experimental confirmation of this hypothesis has been lacking for trehalose/glycerol mixtures or any other mixtures of this nature.
25955786	3	79	theme	experimental	704:715	arg1	confirmation					717:728	experimental confirmation	704:728	experimental confirmation of this hypothesis	704:747	It was also recognized that the fast dynamics should, in theory, be related to the fragility of these glass formers, but experimental confirmation of this hypothesis has been lacking for trehalose/glycerol mixtures or any other mixtures of this nature.
25955786	1	80	theme	final	307:311	arg1	transition					319:328	the final glass transition	303:328	the final glass transition temperature (Tg)	303:345	Approximately a decade ago it was observed that adding a small amount (5 wt %) of glycerol to trehalose could substantially improve the stability of enzymes stored in these glasses even though the final glass transition temperature (Tg) was reduced by ∼20 K.
25955786	5	81	theme	trehalose	1214:1222	arg1	trehalose					1214:1222	trehalose	1214:1222	trehalose	1214:1222	It was found that the fragility index correlated with the mass fraction of trehalose in a nonmonotonic manner, with a local minimum between 87.5 and 95 wt % of trehalose, whereas the composition dependence of Tg was found to follow a Gordon-Taylor-like relationship, with no local minimum.
25955786	5	81	theme	trehalose	1214:1222	arg1	%					1209:1209	87.5 and 95 wt %	1194:1209	%	1209:1209	It was found that the fragility index correlated with the mass fraction of trehalose in a nonmonotonic manner, with a local minimum between 87.5 and 95 wt % of trehalose, whereas the composition dependence of Tg was found to follow a Gordon-Taylor-like relationship, with no local minimum.
25955786	5	82	theme	mass	1112:1115	arg1	fraction					1117:1124	the mass fraction	1108:1124	the mass fraction of trehalose in a nonmonotonic manner	1108:1162	It was found that the fragility index correlated with the mass fraction of trehalose in a nonmonotonic manner, with a local minimum between 87.5 and 95 wt % of trehalose, whereas the composition dependence of Tg was found to follow a Gordon-Taylor-like relationship, with no local minimum.
27543035	5	0	theme	high	738:741	arg1	temperature					743:753	high temperature	738:753	high temperature	738:753	Significant deacetylation of chitosan over the period needed for dissolution at high temperature was observed by NMR spectroscopy in DCl by about 20% of the initial DA value.
27543035	6	1	from	possible	891:898	arg1	state					918:922	the solid state	908:922	the solid state (with a precision within 1% on the DA % scale above a DA of 10%)	908:987	Accurate DA determination by NMR spectroscopy may thus be possible only in the solid state (with a precision within 1% on the DA % scale above a DA of 10%).
27543035	1	2	theme	true	139:142	arg1	solution					145:152	a "true" solution	136:152	a "true" solution	136:152	The dissolution of polysaccharides is notoriously challenging, especially when one needs a "true" solution.
27543035	4	3	theme	used	550:553	arg1	AcOH					576:579	AcOH	576:579	AcOH	576:579	Aqueous HCl dissolves around 15% more chitosan than the commonly used aqueous acetic acid (AcOH), however aggregates were detected in SEC suggesting incomplete dissolution.
27543035	4	3	theme	used	550:553	arg1	acid					570:573	the commonly used aqueous acetic acid	537:573	the commonly used aqueous acetic acid (AcOH)	537:580	Aqueous HCl dissolves around 15% more chitosan than the commonly used aqueous acetic acid (AcOH), however aggregates were detected in SEC suggesting incomplete dissolution.
27543035	7	4	theme	true	1105:1108	arg1	solution					1111:1118	a "true" solution	1102:1118	a "true" solution of chitosan	1102:1130	Overall a compromise between maximum solubilization and minimum degradation is required in attempting to obtain a "true" solution of chitosan.
27543035	4	5	theme	HCl	493:495	arg1	dissolves					497:505	Aqueous HCl dissolves	485:505	Aqueous HCl dissolves around 15% more chitosan than the commonly used aqueous acetic acid (AcOH), however aggregates	485:600	Aqueous HCl dissolves around 15% more chitosan than the commonly used aqueous acetic acid (AcOH), however aggregates were detected in SEC suggesting incomplete dissolution.
27543035	1	6	theme	"	143:143	arg1	solution					145:152	a "true" solution	136:152	a "true" solution	136:152	The dissolution of polysaccharides is notoriously challenging, especially when one needs a "true" solution.
27543035	3	7	theme	size-exclusion	328:341	arg1	SEC					359:361	SEC	359:361	SEC	359:361	The dissolution of chitosan was investigated by visual observation, size-exclusion chromatography (SEC), pressure mobilization (PM), free-solution capillary electrophoresis (CE) and real-time solution-state NMR spectroscopy.
27543035	3	7	theme	size-exclusion	328:341	arg1	chromatography					343:356	size-exclusion chromatography	328:356	size-exclusion chromatography (SEC)	328:362	The dissolution of chitosan was investigated by visual observation, size-exclusion chromatography (SEC), pressure mobilization (PM), free-solution capillary electrophoresis (CE) and real-time solution-state NMR spectroscopy.
27543035	4	8	theme	Aqueous	485:491	arg1	dissolves					497:505	Aqueous HCl dissolves	485:505	Aqueous HCl dissolves around 15% more chitosan than the commonly used aqueous acetic acid (AcOH), however aggregates	485:600	Aqueous HCl dissolves around 15% more chitosan than the commonly used aqueous acetic acid (AcOH), however aggregates were detected in SEC suggesting incomplete dissolution.
27543035	7	9	theme	maximum	1019:1025	arg1	solubilization					1027:1040	maximum solubilization	1019:1040	maximum solubilization	1019:1040	Overall a compromise between maximum solubilization and minimum degradation is required in attempting to obtain a "true" solution of chitosan.
27543035	4	10	theme	acetic	563:568	arg1	AcOH					576:579	AcOH	576:579	AcOH	576:579	Aqueous HCl dissolves around 15% more chitosan than the commonly used aqueous acetic acid (AcOH), however aggregates were detected in SEC suggesting incomplete dissolution.
27543035	4	10	theme	acetic	563:568	arg1	acid					570:573	the commonly used aqueous acetic acid	537:573	the commonly used aqueous acetic acid (AcOH)	537:580	Aqueous HCl dissolves around 15% more chitosan than the commonly used aqueous acetic acid (AcOH), however aggregates were detected in SEC suggesting incomplete dissolution.
27543035	6	11	from	%	950:950	arg1	scale					964:968	the DA % scale	955:968	the DA % scale above a DA of 10%	955:986	Accurate DA determination by NMR spectroscopy may thus be possible only in the solid state (with a precision within 1% on the DA % scale above a DA of 10%).
27543035	6	12	theme	%	986:986	arg1	DA					978:979	a DA	976:979	a DA of 10%	976:986	Accurate DA determination by NMR spectroscopy may thus be possible only in the solid state (with a precision within 1% on the DA % scale above a DA of 10%).
27543035	4	13	theme	aqueous	555:561	arg1	AcOH					576:579	AcOH	576:579	AcOH	576:579	Aqueous HCl dissolves around 15% more chitosan than the commonly used aqueous acetic acid (AcOH), however aggregates were detected in SEC suggesting incomplete dissolution.
27543035	4	13	theme	aqueous	555:561	arg1	acid					570:573	the commonly used aqueous acetic acid	537:573	the commonly used aqueous acetic acid (AcOH)	537:580	Aqueous HCl dissolves around 15% more chitosan than the commonly used aqueous acetic acid (AcOH), however aggregates were detected in SEC suggesting incomplete dissolution.
27543035	6	14	theme	DA	842:843	arg1	determination					845:857	Accurate DA determination	833:857	Accurate DA determination by NMR spectroscopy	833:877	Accurate DA determination by NMR spectroscopy may thus be possible only in the solid state (with a precision within 1% on the DA % scale above a DA of 10%).
27543035	6	15	with	state	918:922	arg1	precision					932:940	a precision	930:940	a precision within 1% on the DA % scale above a DA of 10%	930:986	Accurate DA determination by NMR spectroscopy may thus be possible only in the solid state (with a precision within 1% on the DA % scale above a DA of 10%).
27543035	3	16	theme	free-solution	393:405	arg1	CE					434:435	CE	434:435	CE	434:435	The dissolution of chitosan was investigated by visual observation, size-exclusion chromatography (SEC), pressure mobilization (PM), free-solution capillary electrophoresis (CE) and real-time solution-state NMR spectroscopy.
27543035	3	16	theme	free-solution	393:405	arg1	electrophoresis					417:431	free-solution capillary electrophoresis	393:431	free-solution capillary electrophoresis (CE)	393:436	The dissolution of chitosan was investigated by visual observation, size-exclusion chromatography (SEC), pressure mobilization (PM), free-solution capillary electrophoresis (CE) and real-time solution-state NMR spectroscopy.
27543035	5	17	theme	NMR	771:773	arg1	spectroscopy					775:786	NMR spectroscopy	771:786	NMR spectroscopy	771:786	Significant deacetylation of chitosan over the period needed for dissolution at high temperature was observed by NMR spectroscopy in DCl by about 20% of the initial DA value.
27543035	4	18	dep	chitosan	523:530	arg1	%					516:516	15%	514:516	15%	514:516	Aqueous HCl dissolves around 15% more chitosan than the commonly used aqueous acetic acid (AcOH), however aggregates were detected in SEC suggesting incomplete dissolution.
27543035	7	19	dep	a	998:998	arg1	compromise					1000:1009	compromise	1000:1009	compromise	1000:1009	Overall a compromise between maximum solubilization and minimum degradation is required in attempting to obtain a "true" solution of chitosan.
27543035	5	20	located	observed	759:766	arg1	DCl					791:793	DCl	791:793	DCl	791:793	Significant deacetylation of chitosan over the period needed for dissolution at high temperature was observed by NMR spectroscopy in DCl by about 20% of the initial DA value.
27543035	5	20	located	observed	759:766	arg2	deacetylation					670:682	Significant deacetylation	658:682	Significant deacetylation of chitosan over the period needed for dissolution at high temperature	658:753	Significant deacetylation of chitosan over the period needed for dissolution at high temperature was observed by NMR spectroscopy in DCl by about 20% of the initial DA value.
27543035	1	21	theme	polysaccharides	66:80	arg1	dissolution					51:61	The dissolution	47:61	The dissolution of polysaccharides	47:80	The dissolution of polysaccharides is notoriously challenging, especially when one needs a "true" solution.
27543035	3	22	theme	capillary	407:415	arg1	CE					434:435	CE	434:435	CE	434:435	The dissolution of chitosan was investigated by visual observation, size-exclusion chromatography (SEC), pressure mobilization (PM), free-solution capillary electrophoresis (CE) and real-time solution-state NMR spectroscopy.
27543035	3	22	theme	capillary	407:415	arg1	electrophoresis					417:431	free-solution capillary electrophoresis	393:431	free-solution capillary electrophoresis (CE)	393:436	The dissolution of chitosan was investigated by visual observation, size-exclusion chromatography (SEC), pressure mobilization (PM), free-solution capillary electrophoresis (CE) and real-time solution-state NMR spectroscopy.
27543035	6	23	from	state	918:922	arg1	possible					891:898	possible	891:898	possible	891:898	Accurate DA determination by NMR spectroscopy may thus be possible only in the solid state (with a precision within 1% on the DA % scale above a DA of 10%).
27543035	5	24	theme	DA	823:824	arg1	value					826:830	the initial DA value	811:830	the initial DA value	811:830	Significant deacetylation of chitosan over the period needed for dissolution at high temperature was observed by NMR spectroscopy in DCl by about 20% of the initial DA value.
27543035	6	25	theme	NMR	862:864	arg1	spectroscopy					866:877	NMR spectroscopy	862:877	NMR spectroscopy	862:877	Accurate DA determination by NMR spectroscopy may thus be possible only in the solid state (with a precision within 1% on the DA % scale above a DA of 10%).
27543035	4	26	theme	chitosan	523:530	arg1	aggregates					591:600	15% more chitosan than the commonly used aqueous acetic acid (AcOH), however aggregates	514:600	15% more chitosan than the commonly used aqueous acetic acid (AcOH), however aggregates	514:600	Aqueous HCl dissolves around 15% more chitosan than the commonly used aqueous acetic acid (AcOH), however aggregates were detected in SEC suggesting incomplete dissolution.
27543035	5	27	theme	value	826:830	arg1	%					806:806	about 20%	798:806	about 20% of the initial DA value	798:830	Significant deacetylation of chitosan over the period needed for dissolution at high temperature was observed by NMR spectroscopy in DCl by about 20% of the initial DA value.
27543035	5	27	theme	value	826:830	arg1	value					826:830	the initial DA value	811:830	the initial DA value	811:830	Significant deacetylation of chitosan over the period needed for dissolution at high temperature was observed by NMR spectroscopy in DCl by about 20% of the initial DA value.
27543035	0	28	theme	biased	15:20	arg1	dissolution					22:32	a less biased dissolution	8:32	a less biased dissolution of chitosan	8:44	Towards a less biased dissolution of chitosan.
27543035	6	29	theme	solid	912:916	arg1	state					918:922	the solid state	908:922	the solid state (with a precision within 1% on the DA % scale above a DA of 10%)	908:987	Accurate DA determination by NMR spectroscopy may thus be possible only in the solid state (with a precision within 1% on the DA % scale above a DA of 10%).
27543035	8	30	theme	average	1199:1205	arg1	DA					1207:1208	the average DA	1195:1208	the average DA	1195:1208	The completeness of the dissolution may be more influenced by the average DA than by molar mass.
27543035	4	31	located	detected	607:614	arg2	dissolves					497:505	Aqueous HCl dissolves	485:505	Aqueous HCl dissolves around 15% more chitosan than the commonly used aqueous acetic acid (AcOH), however aggregates	485:600	Aqueous HCl dissolves around 15% more chitosan than the commonly used aqueous acetic acid (AcOH), however aggregates were detected in SEC suggesting incomplete dissolution.
27543035	4	31	located	detected	607:614	arg1	SEC					619:621	SEC	619:621	SEC	619:621	Aqueous HCl dissolves around 15% more chitosan than the commonly used aqueous acetic acid (AcOH), however aggregates were detected in SEC suggesting incomplete dissolution.
27543035	7	32	theme	chitosan	1123:1130	arg1	solution					1111:1118	a "true" solution	1102:1118	a "true" solution of chitosan	1102:1130	Overall a compromise between maximum solubilization and minimum degradation is required in attempting to obtain a "true" solution of chitosan.
27543035	6	33	theme	%	962:962	arg1	scale					964:968	the DA % scale	955:968	the DA % scale above a DA of 10%	955:986	Accurate DA determination by NMR spectroscopy may thus be possible only in the solid state (with a precision within 1% on the DA % scale above a DA of 10%).
27543035	8	34	theme	molar	1218:1222	arg1	mass					1224:1227	molar mass	1218:1227	molar mass	1218:1227	The completeness of the dissolution may be more influenced by the average DA than by molar mass.
27543035	5	35	theme	chitosan	687:694	arg1	deacetylation					670:682	Significant deacetylation	658:682	Significant deacetylation of chitosan over the period needed for dissolution at high temperature	658:753	Significant deacetylation of chitosan over the period needed for dissolution at high temperature was observed by NMR spectroscopy in DCl by about 20% of the initial DA value.
27543035	8	36	theme	dissolution	1157:1167	arg1	completeness					1137:1148	The completeness	1133:1148	The completeness of the dissolution	1133:1167	The completeness of the dissolution may be more influenced by the average DA than by molar mass.
27543035	6	37	theme	DA	959:960	arg1	scale					964:968	the DA % scale	955:968	the DA % scale above a DA of 10%	955:986	Accurate DA determination by NMR spectroscopy may thus be possible only in the solid state (with a precision within 1% on the DA % scale above a DA of 10%).
27543035	7	38	theme	minimum	1046:1052	arg1	degradation					1054:1064	minimum degradation	1046:1064	minimum degradation	1046:1064	Overall a compromise between maximum solubilization and minimum degradation is required in attempting to obtain a "true" solution of chitosan.
27543035	3	39	theme	pressure	365:372	arg1	mobilization					374:385	pressure mobilization	365:385	pressure mobilization (PM)	365:390	The dissolution of chitosan was investigated by visual observation, size-exclusion chromatography (SEC), pressure mobilization (PM), free-solution capillary electrophoresis (CE) and real-time solution-state NMR spectroscopy.
27543035	3	39	theme	pressure	365:372	arg1	PM					388:389	PM	388:389	PM	388:389	The dissolution of chitosan was investigated by visual observation, size-exclusion chromatography (SEC), pressure mobilization (PM), free-solution capillary electrophoresis (CE) and real-time solution-state NMR spectroscopy.
27543035	6	40	theme	Accurate	833:840	arg1	determination					845:857	Accurate DA determination	833:857	Accurate DA determination by NMR spectroscopy	833:877	Accurate DA determination by NMR spectroscopy may thus be possible only in the solid state (with a precision within 1% on the DA % scale above a DA of 10%).
27543035	3	41	theme	solution-state	452:465	arg1	spectroscopy					471:482	real-time solution-state NMR spectroscopy	442:482	real-time solution-state NMR spectroscopy	442:482	The dissolution of chitosan was investigated by visual observation, size-exclusion chromatography (SEC), pressure mobilization (PM), free-solution capillary electrophoresis (CE) and real-time solution-state NMR spectroscopy.
27543035	0	42	theme	chitosan	37:44	arg1	dissolution					22:32	a less biased dissolution	8:32	a less biased dissolution of chitosan	8:44	Towards a less biased dissolution of chitosan.
27543035	3	43	theme	chitosan	279:286	arg1	dissolution					264:274	The dissolution	260:274	The dissolution of chitosan	260:286	The dissolution of chitosan was investigated by visual observation, size-exclusion chromatography (SEC), pressure mobilization (PM), free-solution capillary electrophoresis (CE) and real-time solution-state NMR spectroscopy.
27543035	3	44	theme	NMR	467:469	arg1	spectroscopy					471:482	real-time solution-state NMR spectroscopy	442:482	real-time solution-state NMR spectroscopy	442:482	The dissolution of chitosan was investigated by visual observation, size-exclusion chromatography (SEC), pressure mobilization (PM), free-solution capillary electrophoresis (CE) and real-time solution-state NMR spectroscopy.
27543035	5	45	from	temperature	743:753	arg1	dissolution					723:733	dissolution	723:733	dissolution at high temperature	723:753	Significant deacetylation of chitosan over the period needed for dissolution at high temperature was observed by NMR spectroscopy in DCl by about 20% of the initial DA value.
27543035	2	46	theme	acetylation	242:252	arg1	degree					232:237	degree	232:237	degree of acetylation (DA)	232:257	Factors influencing chitosan's solubility include composition, also known as degree of acetylation (DA).
27543035	5	47	theme	Significant	658:668	arg1	deacetylation					670:682	Significant deacetylation	658:682	Significant deacetylation of chitosan over the period needed for dissolution at high temperature	658:753	Significant deacetylation of chitosan over the period needed for dissolution at high temperature was observed by NMR spectroscopy in DCl by about 20% of the initial DA value.
27543035	3	48	theme	real-time	442:450	arg1	spectroscopy					471:482	real-time solution-state NMR spectroscopy	442:482	real-time solution-state NMR spectroscopy	442:482	The dissolution of chitosan was investigated by visual observation, size-exclusion chromatography (SEC), pressure mobilization (PM), free-solution capillary electrophoresis (CE) and real-time solution-state NMR spectroscopy.
27543035	7	49	theme	"	1109:1109	arg1	solution					1111:1118	a "true" solution	1102:1118	a "true" solution of chitosan	1102:1130	Overall a compromise between maximum solubilization and minimum degradation is required in attempting to obtain a "true" solution of chitosan.
27543035	5	50	theme	initial	815:821	arg1	value					826:830	the initial DA value	811:830	the initial DA value	811:830	Significant deacetylation of chitosan over the period needed for dissolution at high temperature was observed by NMR spectroscopy in DCl by about 20% of the initial DA value.
27543035	3	51	theme	visual	308:313	arg1	observation					315:325	visual observation	308:325	visual observation	308:325	The dissolution of chitosan was investigated by visual observation, size-exclusion chromatography (SEC), pressure mobilization (PM), free-solution capillary electrophoresis (CE) and real-time solution-state NMR spectroscopy.
27543035	4	52	theme	incomplete	634:643	arg1	dissolution					645:655	incomplete dissolution	634:655	incomplete dissolution	634:655	Aqueous HCl dissolves around 15% more chitosan than the commonly used aqueous acetic acid (AcOH), however aggregates were detected in SEC suggesting incomplete dissolution.
27497655	8	0	theme	affinity	1204:1211	arg1	chromatography					1213:1226	AT affinity chromatography	1201:1226	AT affinity chromatography	1201:1226	Heparinase digestion, AT affinity chromatography, gel permeation chromatography, anion exchange chromatography, and nuclear magnetic resonance methodologies were used.
27497655	10	1	theme	oligosaccharides	1646:1661	arg1	potency					1724:1730	the inhibitory potency	1709:1730	the inhibitory potency	1709:1730	These differences influence the strength of the AT-binding affinity of the individual oligosaccharides, their ability to activate AT and, therefore, the inhibitory potency on the proteases of the coagulation cascade.
27497655	10	1	theme	oligosaccharides	1646:1661	arg1	ability					1670:1676	their ability to activate AT and, therefore	1664:1706	their ability to activate AT and, therefore	1664:1706	These differences influence the strength of the AT-binding affinity of the individual oligosaccharides, their ability to activate AT and, therefore, the inhibitory potency on the proteases of the coagulation cascade.
27497655	10	1	theme	oligosaccharides	1646:1661	arg1	affinity					1619:1626	the AT-binding affinity	1604:1626	the AT-binding affinity of the individual oligosaccharides	1604:1661	These differences influence the strength of the AT-binding affinity of the individual oligosaccharides, their ability to activate AT and, therefore, the inhibitory potency on the proteases of the coagulation cascade.
27497655	2	2	theme	heparin	355:361	arg1	activity					343:350	The antithrombotic activity	324:350	The antithrombotic activity of heparin	324:361	The antithrombotic activity of heparin is mediated mainly through its activation of antithrombin (AT) and subsequent inhibition of coagulation factors.
27497655	9	3	theme	USP	1518:1520	arg1	selectivity					1425:1435	the cleavage selectivity	1412:1435	the cleavage selectivity around the heparin AT-binding sequences of the Teva Enoxaparin Sodium Injection, USP and the originator Sanofi enoxaparin	1412:1557	The results indicated differences in oligosaccharides related to the cleavage selectivity around the heparin AT-binding sequences of the Teva Enoxaparin Sodium Injection, USP and the originator Sanofi enoxaparin.
27497655	3	4	theme	uronic	556:561	arg1	acid					563:566	its alternating uronic acid	540:566	its alternating uronic acid	540:566	Heparin is a complex heteropolymer and the sulfation pattern of its alternating uronic acid and glucosamine sugar units is a major factor influencing its biological activity.
27497655	8	5	theme	permeation	1233:1242	arg1	chromatography					1244:1257	gel permeation chromatography	1229:1257	gel permeation chromatography	1229:1257	Heparinase digestion, AT affinity chromatography, gel permeation chromatography, anion exchange chromatography, and nuclear magnetic resonance methodologies were used.
27497655	8	6	theme	resonance	1312:1320	arg1	methodologies					1322:1334	Heparinase digestion, AT affinity chromatography, gel permeation chromatography, anion exchange chromatography, and nuclear magnetic resonance methodologies	1179:1334	Heparinase digestion, AT affinity chromatography, gel permeation chromatography, anion exchange chromatography, and nuclear magnetic resonance methodologies	1179:1334	Heparinase digestion, AT affinity chromatography, gel permeation chromatography, anion exchange chromatography, and nuclear magnetic resonance methodologies were used.
27497655	9	7	theme	Sanofi	1541:1546	arg1	enoxaparin					1548:1557	the originator Sanofi enoxaparin	1526:1557	the originator Sanofi enoxaparin	1526:1557	The results indicated differences in oligosaccharides related to the cleavage selectivity around the heparin AT-binding sequences of the Teva Enoxaparin Sodium Injection, USP and the originator Sanofi enoxaparin.
27497655	7	8	theme	generic	1136:1142	arg1	enoxaparin					1144:1153	a new generic enoxaparin	1130:1153	a new generic enoxaparin commercialized by Teva	1130:1176	The current study was performed to assess potential differences between the originator enoxaparin and a new generic enoxaparin commercialized by Teva.
27497655	3	9	theme	sugar	584:588	arg1	units					590:594	glucosamine sugar units	572:594	glucosamine sugar units	572:594	Heparin is a complex heteropolymer and the sulfation pattern of its alternating uronic acid and glucosamine sugar units is a major factor influencing its biological activity.
27497655	5	10	theme	biological	911:920	arg1	activity					922:929	its biological activity	907:929	its biological activity	907:929	This is important since enoxaparin is prepared by depolymerizing the heparin with the aim of optimizing its biological activity and safety.
27497655	8	11	theme	exchange	1266:1273	arg1	chromatography					1275:1288	anion exchange chromatography	1260:1288	anion exchange chromatography	1260:1288	Heparinase digestion, AT affinity chromatography, gel permeation chromatography, anion exchange chromatography, and nuclear magnetic resonance methodologies were used.
27497655	6	12	theme	biological	996:1005	arg1	activity					1007:1014	its biological activity	992:1014	its biological activity	992:1014	Changes during its manufacture could thus affect its biological activity and safety.
27497655	4	13	theme	manufacturing	655:667	arg1	process					669:675	The manufacturing process	651:675	The manufacturing process itself	651:682	The manufacturing process itself is associated with the introduction of exogenous microheterogeneities that may further affect its biological efficacy.
27497655	0	14	theme	antithrombin-binding	117:136	arg1	components					138:147	antithrombin-binding components	117:147	antithrombin-binding components	117:147	Analytical comparison of a US generic enoxaparin with the originator product: The focus on comparative assessment of antithrombin-binding components.
27497655	3	15	theme	major	601:605	arg1	factor					607:612	a major factor	599:612	a major factor influencing its biological activity	599:648	Heparin is a complex heteropolymer and the sulfation pattern of its alternating uronic acid and glucosamine sugar units is a major factor influencing its biological activity.
27497655	3	15	theme	major	601:605	arg1	pattern					529:535	the sulfation pattern	515:535	the sulfation pattern of its alternating uronic acid and glucosamine sugar units	515:594	Heparin is a complex heteropolymer and the sulfation pattern of its alternating uronic acid and glucosamine sugar units is a major factor influencing its biological activity.
27497655	8	16	theme	nuclear	1295:1301	arg1	resonance					1312:1320	nuclear magnetic resonance	1295:1320	nuclear magnetic resonance	1295:1320	Heparinase digestion, AT affinity chromatography, gel permeation chromatography, anion exchange chromatography, and nuclear magnetic resonance methodologies were used.
27497655	4	17	theme	microheterogeneities	733:752	arg1	introduction					707:718	the introduction	703:718	the introduction of exogenous microheterogeneities that may further affect its biological efficacy	703:800	The manufacturing process itself is associated with the introduction of exogenous microheterogeneities that may further affect its biological efficacy.
27497655	10	18	theme	inhibitory	1713:1722	arg1	potency					1724:1730	the inhibitory potency	1709:1730	the inhibitory potency	1709:1730	These differences influence the strength of the AT-binding affinity of the individual oligosaccharides, their ability to activate AT and, therefore, the inhibitory potency on the proteases of the coagulation cascade.
27497655	10	18	theme	inhibitory	1713:1722	arg1	affinity					1619:1626	the AT-binding affinity	1604:1626	the AT-binding affinity of the individual oligosaccharides	1604:1661	These differences influence the strength of the AT-binding affinity of the individual oligosaccharides, their ability to activate AT and, therefore, the inhibitory potency on the proteases of the coagulation cascade.
27497655	11	19	theme	compositional	1858:1870	arg1	differences					1872:1882	specific compositional differences	1849:1882	specific compositional differences between generics and originator LWMHs	1849:1920	This study, together with other published analytical reports, describes specific compositional differences between generics and originator LWMHs.
27497655	3	20	theme	biological	630:639	arg1	activity					641:648	its biological activity	626:648	its biological activity	626:648	Heparin is a complex heteropolymer and the sulfation pattern of its alternating uronic acid and glucosamine sugar units is a major factor influencing its biological activity.
27497655	7	21	theme	current	1032:1038	arg1	study					1040:1044	The current study	1028:1044	The current study	1028:1044	The current study was performed to assess potential differences between the originator enoxaparin and a new generic enoxaparin commercialized by Teva.
27497655	10	22	from	strength	1592:1599	arg1	proteases					1739:1747	the proteases	1735:1747	the proteases of the coagulation cascade	1735:1774	These differences influence the strength of the AT-binding affinity of the individual oligosaccharides, their ability to activate AT and, therefore, the inhibitory potency on the proteases of the coagulation cascade.
27497655	1	23	dep	prevention	272:281	arg1	the					268:270	the	268:270	the	268:270	Enoxaparin sodium, a low-molecular-weight heparin (LMWH) prepared from porcine intestinal heparin, is widely used for the prevention and treatment of venous thromboembolism.
27497655	9	24	theme	related	1401:1407	arg1	oligosaccharides					1384:1399	oligosaccharides	1384:1399	oligosaccharides related to the cleavage selectivity around the heparin AT-binding sequences of the Teva Enoxaparin Sodium Injection, USP and the originator Sanofi enoxaparin	1384:1557	The results indicated differences in oligosaccharides related to the cleavage selectivity around the heparin AT-binding sequences of the Teva Enoxaparin Sodium Injection, USP and the originator Sanofi enoxaparin.
27497655	10	25	theme	coagulation	1756:1766	arg1	cascade					1768:1774	the coagulation cascade	1752:1774	the coagulation cascade	1752:1774	These differences influence the strength of the AT-binding affinity of the individual oligosaccharides, their ability to activate AT and, therefore, the inhibitory potency on the proteases of the coagulation cascade.
27497655	12	26	theme	clinical	1999:2006	arg1	relevance					2008:2016	any clinical relevance	1995:2016	any clinical relevance	1995:2016	However, it is yet to be established whether such variations might have any clinical relevance.
27497655	0	27	theme	Analytical	0:9	arg1	comparison					11:20	Analytical comparison	0:20	Analytical comparison of a US generic enoxaparin with the originator product: The focus on comparative assessment of antithrombin-binding components.	0:148	Analytical comparison of a US generic enoxaparin with the originator product: The focus on comparative assessment of antithrombin-binding components.
27497655	2	28	theme	subsequent	430:439	arg1	inhibition					441:450	subsequent inhibition	430:450	subsequent inhibition of coagulation factors	430:473	The antithrombotic activity of heparin is mediated mainly through its activation of antithrombin (AT) and subsequent inhibition of coagulation factors.
27497655	10	29	theme	AT-binding	1608:1617	arg1	potency					1724:1730	the inhibitory potency	1709:1730	the inhibitory potency	1709:1730	These differences influence the strength of the AT-binding affinity of the individual oligosaccharides, their ability to activate AT and, therefore, the inhibitory potency on the proteases of the coagulation cascade.
27497655	10	29	theme	AT-binding	1608:1617	arg1	ability					1670:1676	their ability to activate AT and, therefore	1664:1706	their ability to activate AT and, therefore	1664:1706	These differences influence the strength of the AT-binding affinity of the individual oligosaccharides, their ability to activate AT and, therefore, the inhibitory potency on the proteases of the coagulation cascade.
27497655	10	29	theme	AT-binding	1608:1617	arg1	affinity					1619:1626	the AT-binding affinity	1604:1626	the AT-binding affinity of the individual oligosaccharides	1604:1661	These differences influence the strength of the AT-binding affinity of the individual oligosaccharides, their ability to activate AT and, therefore, the inhibitory potency on the proteases of the coagulation cascade.
27497655	4	30	theme	biological	782:791	arg1	efficacy					793:800	its biological efficacy	778:800	its biological efficacy	778:800	The manufacturing process itself is associated with the introduction of exogenous microheterogeneities that may further affect its biological efficacy.
27497655	5	31	with	heparin	872:878	arg1	aim					889:891	the aim	885:891	the aim of optimizing its biological activity and safety	885:940	This is important since enoxaparin is prepared by depolymerizing the heparin with the aim of optimizing its biological activity and safety.
27497655	11	32	theme	other	1803:1807	arg1	reports					1830:1836	other published analytical reports	1803:1836	other published analytical reports	1803:1836	This study, together with other published analytical reports, describes specific compositional differences between generics and originator LWMHs.
27497655	9	33	theme	AT-binding	1456:1465	arg1	sequences					1467:1475	the heparin AT-binding sequences	1444:1475	the heparin AT-binding sequences	1444:1475	The results indicated differences in oligosaccharides related to the cleavage selectivity around the heparin AT-binding sequences of the Teva Enoxaparin Sodium Injection, USP and the originator Sanofi enoxaparin.
27497655	2	34	theme	factors	467:473	arg1	activation					394:403	its activation	390:403	its activation of antithrombin (AT)	390:424	The antithrombotic activity of heparin is mediated mainly through its activation of antithrombin (AT) and subsequent inhibition of coagulation factors.
27497655	2	34	theme	factors	467:473	arg1	inhibition					441:450	subsequent inhibition	430:450	subsequent inhibition of coagulation factors	430:473	The antithrombotic activity of heparin is mediated mainly through its activation of antithrombin (AT) and subsequent inhibition of coagulation factors.
27497655	10	35	theme	individual	1635:1644	arg1	oligosaccharides					1646:1661	the individual oligosaccharides	1631:1661	the individual oligosaccharides	1631:1661	These differences influence the strength of the AT-binding affinity of the individual oligosaccharides, their ability to activate AT and, therefore, the inhibitory potency on the proteases of the coagulation cascade.
27497655	3	36	theme	complex	489:495	arg1	heteropolymer					497:509	a complex heteropolymer	487:509	a complex heteropolymer	487:509	Heparin is a complex heteropolymer and the sulfation pattern of its alternating uronic acid and glucosamine sugar units is a major factor influencing its biological activity.
27497655	3	36	theme	complex	489:495	arg1	Heparin					476:482	Heparin	476:482	Heparin	476:482	Heparin is a complex heteropolymer and the sulfation pattern of its alternating uronic acid and glucosamine sugar units is a major factor influencing its biological activity.
27497655	0	37	theme	enoxaparin	38:47	arg1	comparison					11:20	Analytical comparison	0:20	Analytical comparison of a US generic enoxaparin with the originator product: The focus on comparative assessment of antithrombin-binding components.	0:148	Analytical comparison of a US generic enoxaparin with the originator product: The focus on comparative assessment of antithrombin-binding components.
27497655	1	38	theme	thromboembolism	307:321	arg1	treatment					287:295	treatment	287:295	treatment	287:295	Enoxaparin sodium, a low-molecular-weight heparin (LMWH) prepared from porcine intestinal heparin, is widely used for the prevention and treatment of venous thromboembolism.
27497655	1	38	theme	thromboembolism	307:321	arg1	prevention					272:281	prevention	272:281	prevention	272:281	Enoxaparin sodium, a low-molecular-weight heparin (LMWH) prepared from porcine intestinal heparin, is widely used for the prevention and treatment of venous thromboembolism.
27497655	2	39	theme	antithrombotic	328:341	arg1	activity					343:350	The antithrombotic activity	324:350	The antithrombotic activity of heparin	324:361	The antithrombotic activity of heparin is mediated mainly through its activation of antithrombin (AT) and subsequent inhibition of coagulation factors.
27497655	1	40	theme	porcine	221:227	arg1	heparin					240:246	porcine intestinal heparin	221:246	porcine intestinal heparin	221:246	Enoxaparin sodium, a low-molecular-weight heparin (LMWH) prepared from porcine intestinal heparin, is widely used for the prevention and treatment of venous thromboembolism.
27497655	11	41	theme	analytical	1819:1828	arg1	reports					1830:1836	other published analytical reports	1803:1836	other published analytical reports	1803:1836	This study, together with other published analytical reports, describes specific compositional differences between generics and originator LWMHs.
27497655	3	42	theme	sulfation	519:527	arg1	factor					607:612	a major factor	599:612	a major factor influencing its biological activity	599:648	Heparin is a complex heteropolymer and the sulfation pattern of its alternating uronic acid and glucosamine sugar units is a major factor influencing its biological activity.
27497655	3	42	theme	sulfation	519:527	arg1	pattern					529:535	the sulfation pattern	515:535	the sulfation pattern of its alternating uronic acid and glucosamine sugar units	515:594	Heparin is a complex heteropolymer and the sulfation pattern of its alternating uronic acid and glucosamine sugar units is a major factor influencing its biological activity.
27497655	1	43	theme	low-molecular-weight	171:190	arg1	heparin					192:198	a low-molecular-weight heparin	169:198	a low-molecular-weight heparin (LMWH) prepared from porcine intestinal heparin	169:246	Enoxaparin sodium, a low-molecular-weight heparin (LMWH) prepared from porcine intestinal heparin, is widely used for the prevention and treatment of venous thromboembolism.
27497655	1	43	theme	low-molecular-weight	171:190	arg1	sodium					161:166	sodium	161:166	sodium	161:166	Enoxaparin sodium, a low-molecular-weight heparin (LMWH) prepared from porcine intestinal heparin, is widely used for the prevention and treatment of venous thromboembolism.
27497655	1	43	theme	low-molecular-weight	171:190	arg1	LMWH					201:204	LMWH	201:204	LMWH	201:204	Enoxaparin sodium, a low-molecular-weight heparin (LMWH) prepared from porcine intestinal heparin, is widely used for the prevention and treatment of venous thromboembolism.
27497655	9	44	theme	Sodium	1500:1505	arg1	Injection					1507:1515	the Teva Enoxaparin Sodium Injection	1480:1515	the Teva Enoxaparin Sodium Injection	1480:1515	The results indicated differences in oligosaccharides related to the cleavage selectivity around the heparin AT-binding sequences of the Teva Enoxaparin Sodium Injection, USP and the originator Sanofi enoxaparin.
27497655	8	45	theme	digestion	1190:1198	arg1	methodologies					1322:1334	Heparinase digestion, AT affinity chromatography, gel permeation chromatography, anion exchange chromatography, and nuclear magnetic resonance methodologies	1179:1334	Heparinase digestion, AT affinity chromatography, gel permeation chromatography, anion exchange chromatography, and nuclear magnetic resonance methodologies	1179:1334	Heparinase digestion, AT affinity chromatography, gel permeation chromatography, anion exchange chromatography, and nuclear magnetic resonance methodologies were used.
27497655	8	46	theme	AT	1201:1202	arg1	chromatography					1213:1226	AT affinity chromatography	1201:1226	AT affinity chromatography	1201:1226	Heparinase digestion, AT affinity chromatography, gel permeation chromatography, anion exchange chromatography, and nuclear magnetic resonance methodologies were used.
27497655	3	47	theme	alternating	544:554	arg1	acid					563:566	its alternating uronic acid	540:566	its alternating uronic acid	540:566	Heparin is a complex heteropolymer and the sulfation pattern of its alternating uronic acid and glucosamine sugar units is a major factor influencing its biological activity.
27497655	8	48	theme	chromatography	1213:1226	arg1	methodologies					1322:1334	Heparinase digestion, AT affinity chromatography, gel permeation chromatography, anion exchange chromatography, and nuclear magnetic resonance methodologies	1179:1334	Heparinase digestion, AT affinity chromatography, gel permeation chromatography, anion exchange chromatography, and nuclear magnetic resonance methodologies	1179:1334	Heparinase digestion, AT affinity chromatography, gel permeation chromatography, anion exchange chromatography, and nuclear magnetic resonance methodologies were used.
27497655	9	49	theme	originator	1530:1539	arg1	enoxaparin					1548:1557	the originator Sanofi enoxaparin	1526:1557	the originator Sanofi enoxaparin	1526:1557	The results indicated differences in oligosaccharides related to the cleavage selectivity around the heparin AT-binding sequences of the Teva Enoxaparin Sodium Injection, USP and the originator Sanofi enoxaparin.
27497655	8	50	theme	gel	1229:1231	arg1	chromatography					1244:1257	gel permeation chromatography	1229:1257	gel permeation chromatography	1229:1257	Heparinase digestion, AT affinity chromatography, gel permeation chromatography, anion exchange chromatography, and nuclear magnetic resonance methodologies were used.
27497655	8	51	theme	magnetic	1303:1310	arg1	resonance					1312:1320	nuclear magnetic resonance	1295:1320	nuclear magnetic resonance	1295:1320	Heparinase digestion, AT affinity chromatography, gel permeation chromatography, anion exchange chromatography, and nuclear magnetic resonance methodologies were used.
27497655	7	52	theme	new	1132:1134	arg1	enoxaparin					1144:1153	a new generic enoxaparin	1130:1153	a new generic enoxaparin commercialized by Teva	1130:1176	The current study was performed to assess potential differences between the originator enoxaparin and a new generic enoxaparin commercialized by Teva.
27497655	3	53	theme	glucosamine	572:582	arg1	units					590:594	glucosamine sugar units	572:594	glucosamine sugar units	572:594	Heparin is a complex heteropolymer and the sulfation pattern of its alternating uronic acid and glucosamine sugar units is a major factor influencing its biological activity.
27497655	9	54	theme	enoxaparin	1548:1557	arg1	selectivity					1425:1435	the cleavage selectivity	1412:1435	the cleavage selectivity around the heparin AT-binding sequences of the Teva Enoxaparin Sodium Injection, USP and the originator Sanofi enoxaparin	1412:1557	The results indicated differences in oligosaccharides related to the cleavage selectivity around the heparin AT-binding sequences of the Teva Enoxaparin Sodium Injection, USP and the originator Sanofi enoxaparin.
27497655	8	55	theme	chromatography	1244:1257	arg1	methodologies					1322:1334	Heparinase digestion, AT affinity chromatography, gel permeation chromatography, anion exchange chromatography, and nuclear magnetic resonance methodologies	1179:1334	Heparinase digestion, AT affinity chromatography, gel permeation chromatography, anion exchange chromatography, and nuclear magnetic resonance methodologies	1179:1334	Heparinase digestion, AT affinity chromatography, gel permeation chromatography, anion exchange chromatography, and nuclear magnetic resonance methodologies were used.
27497655	3	56	theme	acid	563:566	arg1	factor					607:612	a major factor	599:612	a major factor influencing its biological activity	599:648	Heparin is a complex heteropolymer and the sulfation pattern of its alternating uronic acid and glucosamine sugar units is a major factor influencing its biological activity.
27497655	3	56	theme	acid	563:566	arg1	pattern					529:535	the sulfation pattern	515:535	the sulfation pattern of its alternating uronic acid and glucosamine sugar units	515:594	Heparin is a complex heteropolymer and the sulfation pattern of its alternating uronic acid and glucosamine sugar units is a major factor influencing its biological activity.
27497655	8	57	theme	anion	1260:1264	arg1	chromatography					1275:1288	anion exchange chromatography	1260:1288	anion exchange chromatography	1260:1288	Heparinase digestion, AT affinity chromatography, gel permeation chromatography, anion exchange chromatography, and nuclear magnetic resonance methodologies were used.
27497655	7	58	theme	originator	1104:1113	arg1	enoxaparin					1115:1124	the originator enoxaparin	1100:1124	the originator enoxaparin	1100:1124	The current study was performed to assess potential differences between the originator enoxaparin and a new generic enoxaparin commercialized by Teva.
27497655	8	59	theme	chromatography	1275:1288	arg1	methodologies					1322:1334	Heparinase digestion, AT affinity chromatography, gel permeation chromatography, anion exchange chromatography, and nuclear magnetic resonance methodologies	1179:1334	Heparinase digestion, AT affinity chromatography, gel permeation chromatography, anion exchange chromatography, and nuclear magnetic resonance methodologies	1179:1334	Heparinase digestion, AT affinity chromatography, gel permeation chromatography, anion exchange chromatography, and nuclear magnetic resonance methodologies were used.
27497655	3	60	theme	units	590:594	arg1	factor					607:612	a major factor	599:612	a major factor influencing its biological activity	599:648	Heparin is a complex heteropolymer and the sulfation pattern of its alternating uronic acid and glucosamine sugar units is a major factor influencing its biological activity.
27497655	3	60	theme	units	590:594	arg1	pattern					529:535	the sulfation pattern	515:535	the sulfation pattern of its alternating uronic acid and glucosamine sugar units	515:594	Heparin is a complex heteropolymer and the sulfation pattern of its alternating uronic acid and glucosamine sugar units is a major factor influencing its biological activity.
27497655	12	61	theme	such	1968:1971	arg1	variations					1973:1982	such variations	1968:1982	such variations	1968:1982	However, it is yet to be established whether such variations might have any clinical relevance.
27497655	9	62	from	differences	1369:1379	arg1	oligosaccharides					1384:1399	oligosaccharides	1384:1399	oligosaccharides related to the cleavage selectivity around the heparin AT-binding sequences of the Teva Enoxaparin Sodium Injection, USP and the originator Sanofi enoxaparin	1384:1557	The results indicated differences in oligosaccharides related to the cleavage selectivity around the heparin AT-binding sequences of the Teva Enoxaparin Sodium Injection, USP and the originator Sanofi enoxaparin.
27497655	4	63	theme	exogenous	723:731	arg1	microheterogeneities					733:752	exogenous microheterogeneities	723:752	exogenous microheterogeneities that may further affect its biological efficacy	723:800	The manufacturing process itself is associated with the introduction of exogenous microheterogeneities that may further affect its biological efficacy.
27497655	7	64	theme	potential	1070:1078	arg1	differences					1080:1090	potential differences	1070:1090	potential differences between the originator enoxaparin	1070:1124	The current study was performed to assess potential differences between the originator enoxaparin and a new generic enoxaparin commercialized by Teva.
27497655	8	65	used	used	1341:1344	arg2	methodologies					1322:1334	Heparinase digestion, AT affinity chromatography, gel permeation chromatography, anion exchange chromatography, and nuclear magnetic resonance methodologies	1179:1334	Heparinase digestion, AT affinity chromatography, gel permeation chromatography, anion exchange chromatography, and nuclear magnetic resonance methodologies	1179:1334	Heparinase digestion, AT affinity chromatography, gel permeation chromatography, anion exchange chromatography, and nuclear magnetic resonance methodologies were used.
27497655	0	66	from	focus	82:86	arg1	assessment					103:112	comparative assessment	91:112	comparative assessment of antithrombin-binding components	91:147	Analytical comparison of a US generic enoxaparin with the originator product: The focus on comparative assessment of antithrombin-binding components.
27497655	0	67	theme	originator	58:67	arg1	product					69:75	the originator product	54:75	the originator product	54:75	Analytical comparison of a US generic enoxaparin with the originator product: The focus on comparative assessment of antithrombin-binding components.
27497655	9	68	theme	Teva	1484:1487	arg1	Injection					1507:1515	the Teva Enoxaparin Sodium Injection	1480:1515	the Teva Enoxaparin Sodium Injection	1480:1515	The results indicated differences in oligosaccharides related to the cleavage selectivity around the heparin AT-binding sequences of the Teva Enoxaparin Sodium Injection, USP and the originator Sanofi enoxaparin.
27497655	11	69	theme	specific	1849:1856	arg1	differences					1872:1882	specific compositional differences	1849:1882	specific compositional differences between generics and originator LWMHs	1849:1920	This study, together with other published analytical reports, describes specific compositional differences between generics and originator LWMHs.
27497655	11	70	theme	originator	1905:1914	arg1	LWMHs					1916:1920	originator LWMHs	1905:1920	originator LWMHs	1905:1920	This study, together with other published analytical reports, describes specific compositional differences between generics and originator LWMHs.
27497655	0	71	theme	comparative	91:101	arg1	assessment					103:112	comparative assessment	91:112	comparative assessment of antithrombin-binding components	91:147	Analytical comparison of a US generic enoxaparin with the originator product: The focus on comparative assessment of antithrombin-binding components.
27497655	1	72	theme	intestinal	229:238	arg1	heparin					240:246	porcine intestinal heparin	221:246	porcine intestinal heparin	221:246	Enoxaparin sodium, a low-molecular-weight heparin (LMWH) prepared from porcine intestinal heparin, is widely used for the prevention and treatment of venous thromboembolism.
27497655	9	73	theme	cleavage	1416:1423	arg1	selectivity					1425:1435	the cleavage selectivity	1412:1435	the cleavage selectivity around the heparin AT-binding sequences of the Teva Enoxaparin Sodium Injection, USP and the originator Sanofi enoxaparin	1412:1557	The results indicated differences in oligosaccharides related to the cleavage selectivity around the heparin AT-binding sequences of the Teva Enoxaparin Sodium Injection, USP and the originator Sanofi enoxaparin.
27497655	0	74	theme	components	138:147	arg1	assessment					103:112	comparative assessment	91:112	comparative assessment of antithrombin-binding components	91:147	Analytical comparison of a US generic enoxaparin with the originator product: The focus on comparative assessment of antithrombin-binding components.
27497655	10	75	theme	cascade	1768:1774	arg1	proteases					1739:1747	the proteases	1735:1747	the proteases of the coagulation cascade	1735:1774	These differences influence the strength of the AT-binding affinity of the individual oligosaccharides, their ability to activate AT and, therefore, the inhibitory potency on the proteases of the coagulation cascade.
27497655	1	76	dep	Enoxaparin	150:159	arg1	heparin					192:198	a low-molecular-weight heparin	169:198	a low-molecular-weight heparin (LMWH) prepared from porcine intestinal heparin	169:246	Enoxaparin sodium, a low-molecular-weight heparin (LMWH) prepared from porcine intestinal heparin, is widely used for the prevention and treatment of venous thromboembolism.
27497655	1	76	dep	Enoxaparin	150:159	arg1	sodium					161:166	sodium	161:166	sodium	161:166	Enoxaparin sodium, a low-molecular-weight heparin (LMWH) prepared from porcine intestinal heparin, is widely used for the prevention and treatment of venous thromboembolism.
27497655	12	77	contain	have	1990:1993	arg2	relevance					2008:2016	any clinical relevance	1995:2016	any clinical relevance	1995:2016	However, it is yet to be established whether such variations might have any clinical relevance.
27497655	12	77	contain	have	1990:1993	arg1	variations					1973:1982	such variations	1968:1982	such variations	1968:1982	However, it is yet to be established whether such variations might have any clinical relevance.
27497655	0	78	theme	generic	30:36	arg1	enoxaparin					38:47	a US generic enoxaparin	25:47	a US generic enoxaparin	25:47	Analytical comparison of a US generic enoxaparin with the originator product: The focus on comparative assessment of antithrombin-binding components.
27497655	9	79	theme	heparin	1448:1454	arg1	sequences					1467:1475	the heparin AT-binding sequences	1444:1475	the heparin AT-binding sequences	1444:1475	The results indicated differences in oligosaccharides related to the cleavage selectivity around the heparin AT-binding sequences of the Teva Enoxaparin Sodium Injection, USP and the originator Sanofi enoxaparin.
27497655	1	80	used	used	259:262	arg2	Enoxaparin					150:159	Enoxaparin sodium, a low-molecular-weight heparin (LMWH) prepared from porcine intestinal heparin,	150:247	Enoxaparin sodium, a low-molecular-weight heparin (LMWH) prepared from porcine intestinal heparin,	150:247	Enoxaparin sodium, a low-molecular-weight heparin (LMWH) prepared from porcine intestinal heparin, is widely used for the prevention and treatment of venous thromboembolism.
27497655	2	81	theme	antithrombin	408:419	arg1	activation					394:403	its activation	390:403	its activation of antithrombin (AT)	390:424	The antithrombotic activity of heparin is mediated mainly through its activation of antithrombin (AT) and subsequent inhibition of coagulation factors.
27497655	2	81	theme	antithrombin	408:419	arg1	inhibition					441:450	subsequent inhibition	430:450	subsequent inhibition of coagulation factors	430:473	The antithrombotic activity of heparin is mediated mainly through its activation of antithrombin (AT) and subsequent inhibition of coagulation factors.
27497655	1	82	theme	venous	300:305	arg1	thromboembolism					307:321	venous thromboembolism	300:321	venous thromboembolism	300:321	Enoxaparin sodium, a low-molecular-weight heparin (LMWH) prepared from porcine intestinal heparin, is widely used for the prevention and treatment of venous thromboembolism.
27497655	2	83	theme	coagulation	455:465	arg1	factors					467:473	coagulation factors	455:473	coagulation factors	455:473	The antithrombotic activity of heparin is mediated mainly through its activation of antithrombin (AT) and subsequent inhibition of coagulation factors.
27497655	0	84	with	comparison	11:20	arg1	product					69:75	the originator product	54:75	the originator product	54:75	Analytical comparison of a US generic enoxaparin with the originator product: The focus on comparative assessment of antithrombin-binding components.
27497655	8	85	theme	Heparinase	1179:1188	arg1	digestion					1190:1198	Heparinase digestion	1179:1198	Heparinase digestion	1179:1198	Heparinase digestion, AT affinity chromatography, gel permeation chromatography, anion exchange chromatography, and nuclear magnetic resonance methodologies were used.
27497655	10	86	theme	affinity	1619:1626	arg1	strength					1592:1599	the strength	1588:1599	the strength of the AT-binding affinity of the individual oligosaccharides, their ability to activate AT and, therefore, the inhibitory potency on the proteases of the coagulation cascade	1588:1774	These differences influence the strength of the AT-binding affinity of the individual oligosaccharides, their ability to activate AT and, therefore, the inhibitory potency on the proteases of the coagulation cascade.
27497655	11	87	theme	published	1809:1817	arg1	reports					1830:1836	other published analytical reports	1803:1836	other published analytical reports	1803:1836	This study, together with other published analytical reports, describes specific compositional differences between generics and originator LWMHs.
27497655	9	88	theme	Enoxaparin	1489:1498	arg1	Injection					1507:1515	the Teva Enoxaparin Sodium Injection	1480:1515	the Teva Enoxaparin Sodium Injection	1480:1515	The results indicated differences in oligosaccharides related to the cleavage selectivity around the heparin AT-binding sequences of the Teva Enoxaparin Sodium Injection, USP and the originator Sanofi enoxaparin.
27497655	0	89	dep	comparison	11:20	arg1	focus					82:86	The focus	78:86	Analytical comparison of a US generic enoxaparin with the originator product: The focus on comparative assessment of antithrombin-binding components.	0:148	Analytical comparison of a US generic enoxaparin with the originator product: The focus on comparative assessment of antithrombin-binding components.
27497655	9	90	theme	Injection	1507:1515	arg1	selectivity					1425:1435	the cleavage selectivity	1412:1435	the cleavage selectivity around the heparin AT-binding sequences of the Teva Enoxaparin Sodium Injection, USP and the originator Sanofi enoxaparin	1412:1557	The results indicated differences in oligosaccharides related to the cleavage selectivity around the heparin AT-binding sequences of the Teva Enoxaparin Sodium Injection, USP and the originator Sanofi enoxaparin.
29556562	4	0	theme	superfamily	807:817	arg1	members					781:787	all previously described members	756:787	all previously described members of the plant PRXs superfamily	756:817	The mature enzyme consists of a single monomer of 308 residues exhibiting the same fold as all previously described members of the plant PRXs superfamily.
29556562	4	0	theme	superfamily	807:817	arg1	fold					748:751	the same fold	739:751	the same fold	739:751	The mature enzyme consists of a single monomer of 308 residues exhibiting the same fold as all previously described members of the plant PRXs superfamily.
29556562	6	1	theme	density	1100:1106	arg1	map					1108:1110	the electron density map	1087:1110	the electron density map	1087:1110	Moreover, seven N-glycosylation sites were found in the structure, and 49 glycans bound to the two ZoPrx molecules found in the asymmetric unit are clearly visible in the electron density map.
29556562	1	2	theme	enzymatic	177:185	arg1	diversity					187:195	Peroxidase (PRXs) enzymatic diversity	159:195	Peroxidase (PRXs) enzymatic diversity	159:195	Understanding Peroxidase (PRXs) enzymatic diversity and functional significance from a three-dimensional point of view is a key point for structural and mechanistic studies.
29556562	7	3	theme	H2O2	1360:1363	arg1	tolerance					1365:1373	the H2O2 tolerance	1356:1373	the H2O2 tolerance of ZoPrx	1356:1382	The comparison of ZoPrx coordinates with homologous enzymes revealed minor structural changes, in which the residue 177 appears to be responsible for enlarging the access to the heme cavity, the only structural finding which may be related to the H2O2 tolerance of ZoPrx and detected by X-ray crystallography.
29556562	8	4	from	range	1473:1477	arg1	synthesis					1519:1527	chemical synthesis	1510:1527	chemical synthesis to environmental biocatalysis	1510:1557	Because of its characteristics, ZoPrx has a broad range of potential applications from chemical synthesis to environmental biocatalysis, thus its aminoacidic sequence, partially completed using the electron density, and the three-dimensional structure itself, become a possible starting point to engineering heme-peroxidases to enhance oxidative stability.
29556562	8	5	theme	three-dimensional	1647:1663	arg1	structure					1665:1673	the three-dimensional structure	1643:1673	the three-dimensional structure itself	1643:1680	Because of its characteristics, ZoPrx has a broad range of potential applications from chemical synthesis to environmental biocatalysis, thus its aminoacidic sequence, partially completed using the electron density, and the three-dimensional structure itself, become a possible starting point to engineering heme-peroxidases to enhance oxidative stability.
29556562	2	6	theme	peroxide	537:544	arg1	presence					516:523	the presence	512:523	the presence of hydrogen peroxide	512:544	In this context, Zo-peroxidase (ZoPrx) a member of the class III peroxidases and secreted by plants, differs from all previously described PRXs because of its remarkable catalytic stability in the presence of hydrogen peroxide.
29556562	8	7	from	synthesis	1519:1527	arg1	range					1473:1477	a broad range	1465:1477	a broad range of potential applications from chemical synthesis to environmental biocatalysis	1465:1557	Because of its characteristics, ZoPrx has a broad range of potential applications from chemical synthesis to environmental biocatalysis, thus its aminoacidic sequence, partially completed using the electron density, and the three-dimensional structure itself, become a possible starting point to engineering heme-peroxidases to enhance oxidative stability.
29556562	8	7	from	synthesis	1519:1527	arg1	applications					1492:1503	potential applications	1482:1503	potential applications from chemical synthesis to environmental biocatalysis	1482:1557	Because of its characteristics, ZoPrx has a broad range of potential applications from chemical synthesis to environmental biocatalysis, thus its aminoacidic sequence, partially completed using the electron density, and the three-dimensional structure itself, become a possible starting point to engineering heme-peroxidases to enhance oxidative stability.
29556562	6	8	theme	ZoPrx	1019:1023	arg1	molecules					1025:1033	the two ZoPrx molecules	1011:1033	the two ZoPrx molecules found in the asymmetric unit	1011:1062	Moreover, seven N-glycosylation sites were found in the structure, and 49 glycans bound to the two ZoPrx molecules found in the asymmetric unit are clearly visible in the electron density map.
29556562	7	9	theme	heme	1291:1294	arg1	finding					1324:1330	the only structural finding	1304:1330	the only structural finding which may be related to the H2O2 tolerance of ZoPrx	1304:1382	The comparison of ZoPrx coordinates with homologous enzymes revealed minor structural changes, in which the residue 177 appears to be responsible for enlarging the access to the heme cavity, the only structural finding which may be related to the H2O2 tolerance of ZoPrx and detected by X-ray crystallography.
29556562	7	9	theme	heme	1291:1294	arg1	cavity					1296:1301	the heme cavity	1287:1301	the heme cavity	1287:1301	The comparison of ZoPrx coordinates with homologous enzymes revealed minor structural changes, in which the residue 177 appears to be responsible for enlarging the access to the heme cavity, the only structural finding which may be related to the H2O2 tolerance of ZoPrx and detected by X-ray crystallography.
29556562	7	9	theme	heme	1291:1294	arg1	related					1345:1351	related	1345:1351	related	1345:1351	The comparison of ZoPrx coordinates with homologous enzymes revealed minor structural changes, in which the residue 177 appears to be responsible for enlarging the access to the heme cavity, the only structural finding which may be related to the H2O2 tolerance of ZoPrx and detected by X-ray crystallography.
29556562	8	10	theme	possible	1692:1699	arg1	point					1710:1714	a possible starting point	1690:1714	a possible starting point to engineering heme-peroxidases to enhance oxidative stability	1690:1777	Because of its characteristics, ZoPrx has a broad range of potential applications from chemical synthesis to environmental biocatalysis, thus its aminoacidic sequence, partially completed using the electron density, and the three-dimensional structure itself, become a possible starting point to engineering heme-peroxidases to enhance oxidative stability.
29556562	4	11	theme	single	697:702	arg1	monomer					704:710	a single monomer	695:710	a single monomer of 308 residues exhibiting the same fold as all previously described members of the plant PRXs superfamily	695:817	The mature enzyme consists of a single monomer of 308 residues exhibiting the same fold as all previously described members of the plant PRXs superfamily.
29556562	4	11	theme	single	697:702	arg1	residues					719:726	308 residues	715:726	308 residues exhibiting the same fold as all previously described members of the plant PRXs superfamily	715:817	The mature enzyme consists of a single monomer of 308 residues exhibiting the same fold as all previously described members of the plant PRXs superfamily.
29556562	6	12	located	found	963:967	arg2	sites					952:956	seven N-glycosylation sites	930:956	seven N-glycosylation sites	930:956	Moreover, seven N-glycosylation sites were found in the structure, and 49 glycans bound to the two ZoPrx molecules found in the asymmetric unit are clearly visible in the electron density map.
29556562	6	12	located	found	963:967	arg1	structure					976:984	the structure	972:984	the structure	972:984	Moreover, seven N-glycosylation sites were found in the structure, and 49 glycans bound to the two ZoPrx molecules found in the asymmetric unit are clearly visible in the electron density map.
29556562	8	13	dep	has	1461:1463	arg1	become					1683:1688	become	1683:1688	become a possible starting point to engineering heme-peroxidases to enhance oxidative stability	1683:1777	Because of its characteristics, ZoPrx has a broad range of potential applications from chemical synthesis to environmental biocatalysis, thus its aminoacidic sequence, partially completed using the electron density, and the three-dimensional structure itself, become a possible starting point to engineering heme-peroxidases to enhance oxidative stability.
29556562	3	14	theme	ZoPrx	606:610	arg1	structure					593:601	the crystallographic structure	572:601	the crystallographic structure of ZoPrx isolated from Japanese radish	572:640	In this work, we present the crystallographic structure of ZoPrx isolated from Japanese radish, at 2.05 Å resolution.
29556562	2	15	theme	remarkable	478:487	arg1	stability					499:507	its remarkable catalytic stability	474:507	its remarkable catalytic stability in the presence of hydrogen peroxide	474:544	In this context, Zo-peroxidase (ZoPrx) a member of the class III peroxidases and secreted by plants, differs from all previously described PRXs because of its remarkable catalytic stability in the presence of hydrogen peroxide.
29556562	0	16	theme	highly-glycosylated	51:69	arg1	peroxidase					71:80	a highly-glycosylated peroxidase	49:80	a highly-glycosylated peroxidase resistant to high concentrations of H2O2 from Japanese radish	49:142	Zo-peroxidase: Crystal structure and sequence of a highly-glycosylated peroxidase resistant to high concentrations of H2O2 from Japanese radish.
29556562	8	17	theme	engineering	1719:1729	arg1	heme-peroxidases					1731:1746	engineering heme-peroxidases	1719:1746	engineering heme-peroxidases	1719:1746	Because of its characteristics, ZoPrx has a broad range of potential applications from chemical synthesis to environmental biocatalysis, thus its aminoacidic sequence, partially completed using the electron density, and the three-dimensional structure itself, become a possible starting point to engineering heme-peroxidases to enhance oxidative stability.
29556562	5	18	theme	heme	855:858	arg1	group					862:866	a heme b group	853:866	a heme b group as the prosthetic group and two Ca2+ binding sites	853:917	Furthermore, the enzyme contains a heme b group as the prosthetic group and two Ca2+ binding sites.
29556562	8	19	contain	has	1461:1463	arg1	ZoPrx					1455:1459	ZoPrx	1455:1459	ZoPrx	1455:1459	Because of its characteristics, ZoPrx has a broad range of potential applications from chemical synthesis to environmental biocatalysis, thus its aminoacidic sequence, partially completed using the electron density, and the three-dimensional structure itself, become a possible starting point to engineering heme-peroxidases to enhance oxidative stability.
29556562	8	19	contain	has	1461:1463	arg2	range					1473:1477	a broad range	1465:1477	a broad range of potential applications from chemical synthesis to environmental biocatalysis	1465:1557	Because of its characteristics, ZoPrx has a broad range of potential applications from chemical synthesis to environmental biocatalysis, thus its aminoacidic sequence, partially completed using the electron density, and the three-dimensional structure itself, become a possible starting point to engineering heme-peroxidases to enhance oxidative stability.
29556562	0	20	theme	resistant	82:90	arg1	peroxidase					71:80	a highly-glycosylated peroxidase	49:80	a highly-glycosylated peroxidase resistant to high concentrations of H2O2 from Japanese radish	49:142	Zo-peroxidase: Crystal structure and sequence of a highly-glycosylated peroxidase resistant to high concentrations of H2O2 from Japanese radish.
29556562	1	21	theme	PRXs	171:174	arg1	diversity					187:195	Peroxidase (PRXs) enzymatic diversity	159:195	Peroxidase (PRXs) enzymatic diversity	159:195	Understanding Peroxidase (PRXs) enzymatic diversity and functional significance from a three-dimensional point of view is a key point for structural and mechanistic studies.
29556562	0	22	theme	high	95:98	arg1	concentrations					100:113	high concentrations	95:113	high concentrations of H2O2 from Japanese radish	95:142	Zo-peroxidase: Crystal structure and sequence of a highly-glycosylated peroxidase resistant to high concentrations of H2O2 from Japanese radish.
29556562	8	23	theme	oxidative	1759:1767	arg1	stability					1769:1777	oxidative stability	1759:1777	oxidative stability	1759:1777	Because of its characteristics, ZoPrx has a broad range of potential applications from chemical synthesis to environmental biocatalysis, thus its aminoacidic sequence, partially completed using the electron density, and the three-dimensional structure itself, become a possible starting point to engineering heme-peroxidases to enhance oxidative stability.
29556562	0	24	theme	peroxidase	71:80	arg1	sequence					37:44	sequence	37:44	sequence	37:44	Zo-peroxidase: Crystal structure and sequence of a highly-glycosylated peroxidase resistant to high concentrations of H2O2 from Japanese radish.
29556562	0	24	theme	peroxidase	71:80	arg1	structure					23:31	Crystal structure	15:31	Crystal structure	15:31	Zo-peroxidase: Crystal structure and sequence of a highly-glycosylated peroxidase resistant to high concentrations of H2O2 from Japanese radish.
29556562	6	25	from	map	1108:1110	arg1	visible					1076:1082	visible	1076:1082	visible	1076:1082	Moreover, seven N-glycosylation sites were found in the structure, and 49 glycans bound to the two ZoPrx molecules found in the asymmetric unit are clearly visible in the electron density map.
29556562	4	26	theme	residues	719:726	arg1	monomer					704:710	a single monomer	695:710	a single monomer of 308 residues exhibiting the same fold as all previously described members of the plant PRXs superfamily	695:817	The mature enzyme consists of a single monomer of 308 residues exhibiting the same fold as all previously described members of the plant PRXs superfamily.
29556562	4	26	theme	residues	719:726	arg1	residues					719:726	308 residues	715:726	308 residues exhibiting the same fold as all previously described members of the plant PRXs superfamily	715:817	The mature enzyme consists of a single monomer of 308 residues exhibiting the same fold as all previously described members of the plant PRXs superfamily.
29556562	0	27	theme	Crystal	15:21	arg1	structure					23:31	Crystal structure	15:31	Crystal structure	15:31	Zo-peroxidase: Crystal structure and sequence of a highly-glycosylated peroxidase resistant to high concentrations of H2O2 from Japanese radish.
29556562	5	28	theme	binding	905:911	arg1	sites					913:917	two Ca2+ binding sites	896:917	two Ca2+ binding sites	896:917	Furthermore, the enzyme contains a heme b group as the prosthetic group and two Ca2+ binding sites.
29556562	4	29	theme	plant	796:800	arg1	superfamily					807:817	the plant PRXs superfamily	792:817	the plant PRXs superfamily	792:817	The mature enzyme consists of a single monomer of 308 residues exhibiting the same fold as all previously described members of the plant PRXs superfamily.
29556562	8	30	theme	potential	1482:1490	arg1	applications					1492:1503	potential applications	1482:1503	potential applications from chemical synthesis to environmental biocatalysis	1482:1557	Because of its characteristics, ZoPrx has a broad range of potential applications from chemical synthesis to environmental biocatalysis, thus its aminoacidic sequence, partially completed using the electron density, and the three-dimensional structure itself, become a possible starting point to engineering heme-peroxidases to enhance oxidative stability.
29556562	6	31	gly	N-glycosylation	936:950	arg2	seven					930:934	seven	930:934	seven	930:934	Moreover, seven N-glycosylation sites were found in the structure, and 49 glycans bound to the two ZoPrx molecules found in the asymmetric unit are clearly visible in the electron density map.
29556562	6	31	gly	N-glycosylation	936:950	arg2	sites					952:956	seven N-glycosylation sites	930:956	seven N-glycosylation sites	930:956	Moreover, seven N-glycosylation sites were found in the structure, and 49 glycans bound to the two ZoPrx molecules found in the asymmetric unit are clearly visible in the electron density map.
29556562	1	32	theme	three-dimensional	232:248	arg1	point					250:254	a three-dimensional point	230:254	a three-dimensional point of view	230:262	Understanding Peroxidase (PRXs) enzymatic diversity and functional significance from a three-dimensional point of view is a key point for structural and mechanistic studies.
29556562	7	33	theme	X-ray	1400:1404	arg1	crystallography					1406:1420	X-ray crystallography	1400:1420	X-ray crystallography	1400:1420	The comparison of ZoPrx coordinates with homologous enzymes revealed minor structural changes, in which the residue 177 appears to be responsible for enlarging the access to the heme cavity, the only structural finding which may be related to the H2O2 tolerance of ZoPrx and detected by X-ray crystallography.
29556562	1	34	theme	Peroxidase	159:168	arg1	diversity					187:195	Peroxidase (PRXs) enzymatic diversity	159:195	Peroxidase (PRXs) enzymatic diversity	159:195	Understanding Peroxidase (PRXs) enzymatic diversity and functional significance from a three-dimensional point of view is a key point for structural and mechanistic studies.
29556562	0	35	from	radish	137:142	arg1	concentrations					100:113	high concentrations	95:113	high concentrations of H2O2 from Japanese radish	95:142	Zo-peroxidase: Crystal structure and sequence of a highly-glycosylated peroxidase resistant to high concentrations of H2O2 from Japanese radish.
29556562	0	35	from	radish	137:142	arg1	H2O2					118:121	H2O2	118:121	H2O2 from Japanese radish	118:142	Zo-peroxidase: Crystal structure and sequence of a highly-glycosylated peroxidase resistant to high concentrations of H2O2 from Japanese radish.
29556562	4	36	theme	described	771:779	arg1	members					781:787	all previously described members	756:787	all previously described members of the plant PRXs superfamily	756:817	The mature enzyme consists of a single monomer of 308 residues exhibiting the same fold as all previously described members of the plant PRXs superfamily.
29556562	4	36	theme	described	771:779	arg1	fold					748:751	the same fold	739:751	the same fold	739:751	The mature enzyme consists of a single monomer of 308 residues exhibiting the same fold as all previously described members of the plant PRXs superfamily.
29556562	0	37	dep	Zo-peroxidase	0:12	arg1	sequence					37:44	sequence	37:44	sequence	37:44	Zo-peroxidase: Crystal structure and sequence of a highly-glycosylated peroxidase resistant to high concentrations of H2O2 from Japanese radish.
29556562	0	37	dep	Zo-peroxidase	0:12	arg1	structure					23:31	Crystal structure	15:31	Crystal structure	15:31	Zo-peroxidase: Crystal structure and sequence of a highly-glycosylated peroxidase resistant to high concentrations of H2O2 from Japanese radish.
29556562	7	38	theme	ZoPrx	1378:1382	arg1	tolerance					1365:1373	the H2O2 tolerance	1356:1373	the H2O2 tolerance of ZoPrx	1356:1382	The comparison of ZoPrx coordinates with homologous enzymes revealed minor structural changes, in which the residue 177 appears to be responsible for enlarging the access to the heme cavity, the only structural finding which may be related to the H2O2 tolerance of ZoPrx and detected by X-ray crystallography.
29556562	7	39	theme	minor	1182:1186	arg1	changes					1199:1205	minor structural changes	1182:1205	minor structural changes	1182:1205	The comparison of ZoPrx coordinates with homologous enzymes revealed minor structural changes, in which the residue 177 appears to be responsible for enlarging the access to the heme cavity, the only structural finding which may be related to the H2O2 tolerance of ZoPrx and detected by X-ray crystallography.
29556562	8	40	theme	environmental	1532:1544	arg1	biocatalysis					1546:1557	environmental biocatalysis	1532:1557	environmental biocatalysis	1532:1557	Because of its characteristics, ZoPrx has a broad range of potential applications from chemical synthesis to environmental biocatalysis, thus its aminoacidic sequence, partially completed using the electron density, and the three-dimensional structure itself, become a possible starting point to engineering heme-peroxidases to enhance oxidative stability.
29556562	1	41	theme	key	269:271	arg1	point					273:277	a key point	267:277	a key point for structural and mechanistic studies	267:316	Understanding Peroxidase (PRXs) enzymatic diversity and functional significance from a three-dimensional point of view is a key point for structural and mechanistic studies.
29556562	8	42	theme	electron	1621:1628	arg1	density					1630:1636	the electron density	1617:1636	the electron density	1617:1636	Because of its characteristics, ZoPrx has a broad range of potential applications from chemical synthesis to environmental biocatalysis, thus its aminoacidic sequence, partially completed using the electron density, and the three-dimensional structure itself, become a possible starting point to engineering heme-peroxidases to enhance oxidative stability.
29556562	4	43	theme	PRXs	802:805	arg1	superfamily					807:817	the plant PRXs superfamily	792:817	the plant PRXs superfamily	792:817	The mature enzyme consists of a single monomer of 308 residues exhibiting the same fold as all previously described members of the plant PRXs superfamily.
29556562	6	44	theme	electron	1091:1098	arg1	map					1108:1110	the electron density map	1087:1110	the electron density map	1087:1110	Moreover, seven N-glycosylation sites were found in the structure, and 49 glycans bound to the two ZoPrx molecules found in the asymmetric unit are clearly visible in the electron density map.
29556562	0	45	theme	Japanese	128:135	arg1	radish					137:142	Japanese radish	128:142	Japanese radish	128:142	Zo-peroxidase: Crystal structure and sequence of a highly-glycosylated peroxidase resistant to high concentrations of H2O2 from Japanese radish.
29556562	1	46	theme	functional	201:210	arg1	significance					212:223	functional significance	201:223	functional significance	201:223	Understanding Peroxidase (PRXs) enzymatic diversity and functional significance from a three-dimensional point of view is a key point for structural and mechanistic studies.
29556562	2	47	theme	hydrogen	528:535	arg1	peroxide					537:544	hydrogen peroxide	528:544	hydrogen peroxide	528:544	In this context, Zo-peroxidase (ZoPrx) a member of the class III peroxidases and secreted by plants, differs from all previously described PRXs because of its remarkable catalytic stability in the presence of hydrogen peroxide.
29556562	6	48	located	found	1035:1039	arg1	unit					1059:1062	the asymmetric unit	1044:1062	the asymmetric unit	1044:1062	Moreover, seven N-glycosylation sites were found in the structure, and 49 glycans bound to the two ZoPrx molecules found in the asymmetric unit are clearly visible in the electron density map.
29556562	6	48	located	found	1035:1039	arg2	molecules					1025:1033	the two ZoPrx molecules	1011:1033	the two ZoPrx molecules found in the asymmetric unit	1011:1062	Moreover, seven N-glycosylation sites were found in the structure, and 49 glycans bound to the two ZoPrx molecules found in the asymmetric unit are clearly visible in the electron density map.
29556562	7	49	theme	structural	1313:1322	arg1	finding					1324:1330	the only structural finding	1304:1330	the only structural finding which may be related to the H2O2 tolerance of ZoPrx	1304:1382	The comparison of ZoPrx coordinates with homologous enzymes revealed minor structural changes, in which the residue 177 appears to be responsible for enlarging the access to the heme cavity, the only structural finding which may be related to the H2O2 tolerance of ZoPrx and detected by X-ray crystallography.
29556562	7	49	theme	structural	1313:1322	arg1	cavity					1296:1301	the heme cavity	1287:1301	the heme cavity	1287:1301	The comparison of ZoPrx coordinates with homologous enzymes revealed minor structural changes, in which the residue 177 appears to be responsible for enlarging the access to the heme cavity, the only structural finding which may be related to the H2O2 tolerance of ZoPrx and detected by X-ray crystallography.
29556562	7	49	theme	structural	1313:1322	arg1	related					1345:1351	related	1345:1351	related	1345:1351	The comparison of ZoPrx coordinates with homologous enzymes revealed minor structural changes, in which the residue 177 appears to be responsible for enlarging the access to the heme cavity, the only structural finding which may be related to the H2O2 tolerance of ZoPrx and detected by X-ray crystallography.
29556562	7	50	with	comparison	1117:1126	arg1	enzymes					1165:1171	homologous enzymes	1154:1171	homologous enzymes	1154:1171	The comparison of ZoPrx coordinates with homologous enzymes revealed minor structural changes, in which the residue 177 appears to be responsible for enlarging the access to the heme cavity, the only structural finding which may be related to the H2O2 tolerance of ZoPrx and detected by X-ray crystallography.
29556562	3	51	attach	isolated	612:619	arg2	ZoPrx					606:610	ZoPrx	606:610	ZoPrx isolated from Japanese radish	606:640	In this work, we present the crystallographic structure of ZoPrx isolated from Japanese radish, at 2.05 Å resolution.
29556562	3	51	attach	isolated	612:619	arg1	radish					635:640	Japanese radish	626:640	Japanese radish	626:640	In this work, we present the crystallographic structure of ZoPrx isolated from Japanese radish, at 2.05 Å resolution.
29556562	2	52	from	stability	499:507	arg1	presence					516:523	the presence	512:523	the presence of hydrogen peroxide	512:544	In this context, Zo-peroxidase (ZoPrx) a member of the class III peroxidases and secreted by plants, differs from all previously described PRXs because of its remarkable catalytic stability in the presence of hydrogen peroxide.
29556562	5	53	theme	prosthetic	875:884	arg1	group					886:890	the prosthetic group	871:890	the prosthetic group	871:890	Furthermore, the enzyme contains a heme b group as the prosthetic group and two Ca2+ binding sites.
29556562	0	54	from	concentrations	100:113	arg1	radish					137:142	Japanese radish	128:142	Japanese radish	128:142	Zo-peroxidase: Crystal structure and sequence of a highly-glycosylated peroxidase resistant to high concentrations of H2O2 from Japanese radish.
29556562	7	55	theme	homologous	1154:1163	arg1	enzymes					1165:1171	homologous enzymes	1154:1171	homologous enzymes	1154:1171	The comparison of ZoPrx coordinates with homologous enzymes revealed minor structural changes, in which the residue 177 appears to be responsible for enlarging the access to the heme cavity, the only structural finding which may be related to the H2O2 tolerance of ZoPrx and detected by X-ray crystallography.
29556562	3	56	theme	crystallographic	576:591	arg1	structure					593:601	the crystallographic structure	572:601	the crystallographic structure of ZoPrx isolated from Japanese radish	572:640	In this work, we present the crystallographic structure of ZoPrx isolated from Japanese radish, at 2.05 Å resolution.
29556562	8	57	theme	starting	1701:1708	arg1	point					1710:1714	a possible starting point	1690:1714	a possible starting point to engineering heme-peroxidases to enhance oxidative stability	1690:1777	Because of its characteristics, ZoPrx has a broad range of potential applications from chemical synthesis to environmental biocatalysis, thus its aminoacidic sequence, partially completed using the electron density, and the three-dimensional structure itself, become a possible starting point to engineering heme-peroxidases to enhance oxidative stability.
29556562	7	58	theme	ZoPrx	1131:1135	arg1	coordinates					1137:1147	ZoPrx coordinates	1131:1147	ZoPrx coordinates	1131:1147	The comparison of ZoPrx coordinates with homologous enzymes revealed minor structural changes, in which the residue 177 appears to be responsible for enlarging the access to the heme cavity, the only structural finding which may be related to the H2O2 tolerance of ZoPrx and detected by X-ray crystallography.
29556562	7	59	theme	coordinates	1137:1147	arg1	comparison					1117:1126	The comparison	1113:1126	The comparison of ZoPrx coordinates with homologous enzymes	1113:1171	The comparison of ZoPrx coordinates with homologous enzymes revealed minor structural changes, in which the residue 177 appears to be responsible for enlarging the access to the heme cavity, the only structural finding which may be related to the H2O2 tolerance of ZoPrx and detected by X-ray crystallography.
29556562	5	60	theme	b	860:860	arg1	group					862:866	a heme b group	853:866	a heme b group as the prosthetic group and two Ca2+ binding sites	853:917	Furthermore, the enzyme contains a heme b group as the prosthetic group and two Ca2+ binding sites.
29556562	3	61	attach	present	564:570	arg1	resolution					653:662	2.05 Å resolution	646:662	2.05 Å resolution	646:662	In this work, we present the crystallographic structure of ZoPrx isolated from Japanese radish, at 2.05 Å resolution.
29556562	3	61	attach	present	564:570	arg2	we					561:562	we	561:562	we	561:562	In this work, we present the crystallographic structure of ZoPrx isolated from Japanese radish, at 2.05 Å resolution.
29556562	3	61	attach	present	564:570	arg1	work					555:558	this work	550:558	this work	550:558	In this work, we present the crystallographic structure of ZoPrx isolated from Japanese radish, at 2.05 Å resolution.
29556562	5	62	contain	contains	844:851	arg2	group					862:866	a heme b group	853:866	a heme b group as the prosthetic group and two Ca2+ binding sites	853:917	Furthermore, the enzyme contains a heme b group as the prosthetic group and two Ca2+ binding sites.
29556562	5	62	contain	contains	844:851	arg1	enzyme					837:842	the enzyme	833:842	the enzyme	833:842	Furthermore, the enzyme contains a heme b group as the prosthetic group and two Ca2+ binding sites.
29556562	4	63	theme	mature	669:674	arg1	enzyme					676:681	The mature enzyme	665:681	The mature enzyme	665:681	The mature enzyme consists of a single monomer of 308 residues exhibiting the same fold as all previously described members of the plant PRXs superfamily.
29556562	0	64	gly	highly-glycosylated	51:69	arg1	peroxidase					71:80	a highly-glycosylated peroxidase	49:80	a highly-glycosylated peroxidase resistant to high concentrations of H2O2 from Japanese radish	49:142	Zo-peroxidase: Crystal structure and sequence of a highly-glycosylated peroxidase resistant to high concentrations of H2O2 from Japanese radish.
29556562	6	65	theme	N-glycosylation	936:950	arg1	sites					952:956	seven N-glycosylation sites	930:956	seven N-glycosylation sites	930:956	Moreover, seven N-glycosylation sites were found in the structure, and 49 glycans bound to the two ZoPrx molecules found in the asymmetric unit are clearly visible in the electron density map.
29556562	0	66	theme	H2O2	118:121	arg1	concentrations					100:113	high concentrations	95:113	high concentrations of H2O2 from Japanese radish	95:142	Zo-peroxidase: Crystal structure and sequence of a highly-glycosylated peroxidase resistant to high concentrations of H2O2 from Japanese radish.
29556562	2	67	theme	catalytic	489:497	arg1	stability					499:507	its remarkable catalytic stability	474:507	its remarkable catalytic stability in the presence of hydrogen peroxide	474:544	In this context, Zo-peroxidase (ZoPrx) a member of the class III peroxidases and secreted by plants, differs from all previously described PRXs because of its remarkable catalytic stability in the presence of hydrogen peroxide.
29556562	4	68	theme	same	743:746	arg1	members					781:787	all previously described members	756:787	all previously described members of the plant PRXs superfamily	756:817	The mature enzyme consists of a single monomer of 308 residues exhibiting the same fold as all previously described members of the plant PRXs superfamily.
29556562	4	68	theme	same	743:746	arg1	fold					748:751	the same fold	739:751	the same fold	739:751	The mature enzyme consists of a single monomer of 308 residues exhibiting the same fold as all previously described members of the plant PRXs superfamily.
29556562	2	69	theme	class	374:378	arg1	peroxidases					384:394	the class III peroxidases	370:394	the class III peroxidases and secreted by plants	370:417	In this context, Zo-peroxidase (ZoPrx) a member of the class III peroxidases and secreted by plants, differs from all previously described PRXs because of its remarkable catalytic stability in the presence of hydrogen peroxide.
29556562	6	70	from	visible	1076:1082	arg1	map					1108:1110	the electron density map	1087:1110	the electron density map	1087:1110	Moreover, seven N-glycosylation sites were found in the structure, and 49 glycans bound to the two ZoPrx molecules found in the asymmetric unit are clearly visible in the electron density map.
29556562	1	71	theme	structural	283:292	arg1	studies					310:316	structural and mechanistic studies	283:316	structural and mechanistic studies	283:316	Understanding Peroxidase (PRXs) enzymatic diversity and functional significance from a three-dimensional point of view is a key point for structural and mechanistic studies.
29556562	5	72	theme	Ca2+	900:903	arg1	sites					913:917	two Ca2+ binding sites	896:917	two Ca2+ binding sites	896:917	Furthermore, the enzyme contains a heme b group as the prosthetic group and two Ca2+ binding sites.
29556562	8	73	theme	broad	1467:1471	arg1	range					1473:1477	a broad range	1465:1477	a broad range of potential applications from chemical synthesis to environmental biocatalysis	1465:1557	Because of its characteristics, ZoPrx has a broad range of potential applications from chemical synthesis to environmental biocatalysis, thus its aminoacidic sequence, partially completed using the electron density, and the three-dimensional structure itself, become a possible starting point to engineering heme-peroxidases to enhance oxidative stability.
29556562	3	74	theme	Japanese	626:633	arg1	radish					635:640	Japanese radish	626:640	Japanese radish	626:640	In this work, we present the crystallographic structure of ZoPrx isolated from Japanese radish, at 2.05 Å resolution.
29556562	2	75	dep	Zo-peroxidase	336:348	arg1	member					360:365	a member	358:365	a member of the class III peroxidases and secreted by plants	358:417	In this context, Zo-peroxidase (ZoPrx) a member of the class III peroxidases and secreted by plants, differs from all previously described PRXs because of its remarkable catalytic stability in the presence of hydrogen peroxide.
29556562	1	76	theme	mechanistic	298:308	arg1	studies					310:316	structural and mechanistic studies	283:316	structural and mechanistic studies	283:316	Understanding Peroxidase (PRXs) enzymatic diversity and functional significance from a three-dimensional point of view is a key point for structural and mechanistic studies.
29556562	7	77	theme	residue	1221:1227	arg1	responsible					1247:1257	responsible	1247:1257	responsible	1247:1257	The comparison of ZoPrx coordinates with homologous enzymes revealed minor structural changes, in which the residue 177 appears to be responsible for enlarging the access to the heme cavity, the only structural finding which may be related to the H2O2 tolerance of ZoPrx and detected by X-ray crystallography.
29556562	7	77	theme	residue	1221:1227	arg1	177					1229:1231	the residue 177	1217:1231	the residue 177	1217:1231	The comparison of ZoPrx coordinates with homologous enzymes revealed minor structural changes, in which the residue 177 appears to be responsible for enlarging the access to the heme cavity, the only structural finding which may be related to the H2O2 tolerance of ZoPrx and detected by X-ray crystallography.
29556562	2	78	theme	described	448:456	arg1	PRXs					458:461	all previously described PRXs	433:461	all previously described PRXs	433:461	In this context, Zo-peroxidase (ZoPrx) a member of the class III peroxidases and secreted by plants, differs from all previously described PRXs because of its remarkable catalytic stability in the presence of hydrogen peroxide.
29556562	2	79	dep	peroxidases	384:394	arg1	secreted					400:407	secreted	400:407	secreted by plants	400:417	In this context, Zo-peroxidase (ZoPrx) a member of the class III peroxidases and secreted by plants, differs from all previously described PRXs because of its remarkable catalytic stability in the presence of hydrogen peroxide.
29556562	2	80	theme	peroxidases	384:394	arg1	member					360:365	a member	358:365	a member of the class III peroxidases and secreted by plants	358:417	In this context, Zo-peroxidase (ZoPrx) a member of the class III peroxidases and secreted by plants, differs from all previously described PRXs because of its remarkable catalytic stability in the presence of hydrogen peroxide.
29556562	8	81	theme	applications	1492:1503	arg1	range					1473:1477	a broad range	1465:1477	a broad range of potential applications from chemical synthesis to environmental biocatalysis	1465:1557	Because of its characteristics, ZoPrx has a broad range of potential applications from chemical synthesis to environmental biocatalysis, thus its aminoacidic sequence, partially completed using the electron density, and the three-dimensional structure itself, become a possible starting point to engineering heme-peroxidases to enhance oxidative stability.
29556562	3	82	theme	2.05 Å	646:651	arg1	resolution					653:662	2.05 Å resolution	646:662	2.05 Å resolution	646:662	In this work, we present the crystallographic structure of ZoPrx isolated from Japanese radish, at 2.05 Å resolution.
29556562	8	83	theme	aminoacidic	1569:1579	arg1	sequence					1581:1588	its aminoacidic sequence	1565:1588	its aminoacidic sequence	1565:1588	Because of its characteristics, ZoPrx has a broad range of potential applications from chemical synthesis to environmental biocatalysis, thus its aminoacidic sequence, partially completed using the electron density, and the three-dimensional structure itself, become a possible starting point to engineering heme-peroxidases to enhance oxidative stability.
29556562	6	84	theme	asymmetric	1048:1057	arg1	unit					1059:1062	the asymmetric unit	1044:1062	the asymmetric unit	1044:1062	Moreover, seven N-glycosylation sites were found in the structure, and 49 glycans bound to the two ZoPrx molecules found in the asymmetric unit are clearly visible in the electron density map.
29556562	8	85	theme	chemical	1510:1517	arg1	synthesis					1519:1527	chemical synthesis	1510:1527	chemical synthesis to environmental biocatalysis	1510:1557	Because of its characteristics, ZoPrx has a broad range of potential applications from chemical synthesis to environmental biocatalysis, thus its aminoacidic sequence, partially completed using the electron density, and the three-dimensional structure itself, become a possible starting point to engineering heme-peroxidases to enhance oxidative stability.
29556562	7	86	theme	structural	1188:1197	arg1	changes					1199:1205	minor structural changes	1182:1205	minor structural changes	1182:1205	The comparison of ZoPrx coordinates with homologous enzymes revealed minor structural changes, in which the residue 177 appears to be responsible for enlarging the access to the heme cavity, the only structural finding which may be related to the H2O2 tolerance of ZoPrx and detected by X-ray crystallography.
29556562	1	87	theme	view	259:262	arg1	point					250:254	a three-dimensional point	230:254	a three-dimensional point of view	230:262	Understanding Peroxidase (PRXs) enzymatic diversity and functional significance from a three-dimensional point of view is a key point for structural and mechanistic studies.
27145129	8	0	theme	spray	951:955	arg1	drying					957:962	spray drying	951:962	spray drying	951:962	The use of inulin during spray drying resulted in greater efficiency of micro-encapsulation than the use of maltodextrin.
27145129	10	1	contain	have	1306:1309	arg2	activity					1331:1338	valuable biological activity	1311:1338	valuable biological activity	1311:1338	CONCLUSION The microcapsules with procyanidins from the bark of hawthorn, as well as the extract of procyanidins, have valuable biological activity, and strong antioxidant and anti-inflammatory properties.
27145129	10	1	contain	have	1306:1309	arg1	microcapsules					1207:1219	The microcapsules	1203:1219	The microcapsules with procyanidins from the bark of hawthorn, as well as the extract of procyanidins,	1203:1304	CONCLUSION The microcapsules with procyanidins from the bark of hawthorn, as well as the extract of procyanidins, have valuable biological activity, and strong antioxidant and anti-inflammatory properties.
27145129	10	1	contain	have	1306:1309	arg2	properties					1386:1395	strong antioxidant and anti-inflammatory properties	1345:1395	strong antioxidant and anti-inflammatory properties	1345:1395	CONCLUSION The microcapsules with procyanidins from the bark of hawthorn, as well as the extract of procyanidins, have valuable biological activity, and strong antioxidant and anti-inflammatory properties.
27145129	0	2	theme	hawthorn	81:88	arg1	Jacq					110:113	Jacq	110:113	Jacq	110:113	Physico-chemical, antioxidant, and anti-inflammatory properties and stability of hawthorn (Crataegus monogyna Jacq.)
27145129	0	2	theme	hawthorn	81:88	arg1	stability					68:76	stability	68:76	stability of hawthorn	68:88	Physico-chemical, antioxidant, and anti-inflammatory properties and stability of hawthorn (Crataegus monogyna Jacq.)
27145129	0	2	theme	hawthorn	81:88	arg1	properties					53:62	anti-inflammatory properties	35:62	anti-inflammatory properties	35:62	Physico-chemical, antioxidant, and anti-inflammatory properties and stability of hawthorn (Crataegus monogyna Jacq.)
27145129	10	3	theme	strong	1345:1350	arg1	properties					1386:1395	strong antioxidant and anti-inflammatory properties	1345:1395	strong antioxidant and anti-inflammatory properties	1345:1395	CONCLUSION The microcapsules with procyanidins from the bark of hawthorn, as well as the extract of procyanidins, have valuable biological activity, and strong antioxidant and anti-inflammatory properties.
27145129	10	4	theme	hawthorn	1256:1263	arg1	bark					1248:1251	the bark	1244:1251	the bark of hawthorn, as well as the extract of procyanidins,	1244:1304	CONCLUSION The microcapsules with procyanidins from the bark of hawthorn, as well as the extract of procyanidins, have valuable biological activity, and strong antioxidant and anti-inflammatory properties.
27145129	5	5	theme	anti-inflammatory	434:450	arg1	properties					452:461	antioxidant and anti-inflammatory properties	418:461	antioxidant and anti-inflammatory properties	418:461	Stability and antioxidant and anti-inflammatory properties of procyanidins before and after micro-encapsulation were estimated.
27145129	10	6	theme	antioxidant	1352:1362	arg1	properties					1386:1395	strong antioxidant and anti-inflammatory properties	1345:1395	strong antioxidant and anti-inflammatory properties	1345:1395	CONCLUSION The microcapsules with procyanidins from the bark of hawthorn, as well as the extract of procyanidins, have valuable biological activity, and strong antioxidant and anti-inflammatory properties.
27145129	7	7	from	mg	823:824	arg1	-epicatechin					877:888	1 g. Among the procyanidins, (-)-epicatechin	845:888	1 g. Among the procyanidins, (-)-epicatechin	845:888	RESULTS Samples before and after micro-encapsulation contained from 651 to 751 mg of procyanidins in 1 g. Among the procyanidins, (-)-epicatechin, dimer B2, and trimer C1 dominated.
27145129	7	7	from	mg	823:824	arg1	B2					897:898	dimer B2	891:898	dimer B2	891:898	RESULTS Samples before and after micro-encapsulation contained from 651 to 751 mg of procyanidins in 1 g. Among the procyanidins, (-)-epicatechin, dimer B2, and trimer C1 dominated.
27145129	7	7	from	mg	823:824	arg1	C1					912:913	trimer C1	905:913	trimer C1	905:913	RESULTS Samples before and after micro-encapsulation contained from 651 to 751 mg of procyanidins in 1 g. Among the procyanidins, (-)-epicatechin, dimer B2, and trimer C1 dominated.
27145129	10	8	with	microcapsules	1207:1219	arg1	procyanidins					1226:1237	procyanidins	1226:1237	procyanidins	1226:1237	CONCLUSION The microcapsules with procyanidins from the bark of hawthorn, as well as the extract of procyanidins, have valuable biological activity, and strong antioxidant and anti-inflammatory properties.
27145129	9	9	theme	procyanidins	1102:1113	arg1	degradation					1087:1097	degradation	1087:1097	degradation of procyanidins	1087:1113	During storage of the samples at 20 °C degradation of procyanidins was observed, whereas at -20 °C and -80 °C concentrations of them increased.
27145129	6	10	theme	type	559:562	arg1	effects					536:542	The effects	532:542	The effects of the carrier type (inulin and maltodextrin) and procyanidins:carrier ratio (1:1, 1:3)	532:630	The effects of the carrier type (inulin and maltodextrin) and procyanidins:carrier ratio (1:1, 1:3) and the influence of storage temperature (20 °C, -20 °C, -80 °C) on the content of procyanidins were evaluated.
27145129	6	10	theme	type	559:562	arg1	influence					640:648	the influence	636:648	the influence of storage temperature (20 °C, -20 °C, -80 °C) on the content of procyanidins	636:726	The effects of the carrier type (inulin and maltodextrin) and procyanidins:carrier ratio (1:1, 1:3) and the influence of storage temperature (20 °C, -20 °C, -80 °C) on the content of procyanidins were evaluated.
27145129	6	11	dep	type	559:562	arg1	maltodextrin					576:587	maltodextrin	576:587	maltodextrin	576:587	The effects of the carrier type (inulin and maltodextrin) and procyanidins:carrier ratio (1:1, 1:3) and the influence of storage temperature (20 °C, -20 °C, -80 °C) on the content of procyanidins were evaluated.
27145129	6	11	dep	type	559:562	arg1	inulin					565:570	inulin	565:570	inulin	565:570	The effects of the carrier type (inulin and maltodextrin) and procyanidins:carrier ratio (1:1, 1:3) and the influence of storage temperature (20 °C, -20 °C, -80 °C) on the content of procyanidins were evaluated.
27145129	7	12	theme	dimer	891:895	arg1	B2					897:898	dimer B2	891:898	dimer B2	891:898	RESULTS Samples before and after micro-encapsulation contained from 651 to 751 mg of procyanidins in 1 g. Among the procyanidins, (-)-epicatechin, dimer B2, and trimer C1 dominated.
27145129	6	13	theme	carrier	551:557	arg1	type					559:562	the carrier type	547:562	the carrier type (inulin and maltodextrin)	547:588	The effects of the carrier type (inulin and maltodextrin) and procyanidins:carrier ratio (1:1, 1:3) and the influence of storage temperature (20 °C, -20 °C, -80 °C) on the content of procyanidins were evaluated.
27145129	8	14	theme	greater	976:982	arg1	efficiency					984:993	greater efficiency	976:993	greater efficiency of micro-encapsulation than the use of maltodextrin	976:1045	The use of inulin during spray drying resulted in greater efficiency of micro-encapsulation than the use of maltodextrin.
27145129	10	15	theme	extract	1281:1287	arg1	bark					1248:1251	the bark	1244:1251	the bark of hawthorn, as well as the extract of procyanidins,	1244:1304	CONCLUSION The microcapsules with procyanidins from the bark of hawthorn, as well as the extract of procyanidins, have valuable biological activity, and strong antioxidant and anti-inflammatory properties.
27145129	4	16	theme	quantitative	295:306	arg1	composition					308:318	Qualitative and quantitative composition	279:318	Qualitative and quantitative composition	279:318	Qualitative and quantitative composition was compared with that of the extract of hawthorn fruit (Crataegus monogyna Jacq.)
27145129	9	17	from	°C	1084:1085	arg1	storage					1055:1061	storage	1055:1061	storage of the samples at 20 °C	1055:1085	During storage of the samples at 20 °C degradation of procyanidins was observed, whereas at -20 °C and -80 °C concentrations of them increased.
27145129	7	18	dep	RESULTS	744:750	arg1	contained					797:805	contained	797:805	contained from 651 to 751 mg of procyanidins in 1 g. Among the procyanidins, (-)-epicatechin, dimer B2, and trimer C1	797:913	RESULTS Samples before and after micro-encapsulation contained from 651 to 751 mg of procyanidins in 1 g. Among the procyanidins, (-)-epicatechin, dimer B2, and trimer C1 dominated.
27145129	0	19	theme	anti-inflammatory	35:51	arg1	Jacq					110:113	Jacq	110:113	Jacq	110:113	Physico-chemical, antioxidant, and anti-inflammatory properties and stability of hawthorn (Crataegus monogyna Jacq.)
27145129	0	19	theme	anti-inflammatory	35:51	arg1	properties					53:62	anti-inflammatory properties	35:62	anti-inflammatory properties	35:62	Physico-chemical, antioxidant, and anti-inflammatory properties and stability of hawthorn (Crataegus monogyna Jacq.)
27145129	7	20	from	C1	912:913	arg1	mg					823:824	651 to 751 mg	812:824	651 to 751 mg of procyanidins in 1 g. Among the procyanidins, (-)-epicatechin, dimer B2, and trimer C1	812:913	RESULTS Samples before and after micro-encapsulation contained from 651 to 751 mg of procyanidins in 1 g. Among the procyanidins, (-)-epicatechin, dimer B2, and trimer C1 dominated.
27145129	10	21	theme	anti-inflammatory	1368:1384	arg1	properties					1386:1395	strong antioxidant and anti-inflammatory properties	1345:1395	strong antioxidant and anti-inflammatory properties	1345:1395	CONCLUSION The microcapsules with procyanidins from the bark of hawthorn, as well as the extract of procyanidins, have valuable biological activity, and strong antioxidant and anti-inflammatory properties.
27145129	10	22	theme	biological	1320:1329	arg1	activity					1331:1338	valuable biological activity	1311:1338	valuable biological activity	1311:1338	CONCLUSION The microcapsules with procyanidins from the bark of hawthorn, as well as the extract of procyanidins, have valuable biological activity, and strong antioxidant and anti-inflammatory properties.
27145129	9	23	dep	observed	1119:1126	arg1	whereas					1129:1135	whereas	1129:1135	whereas	1129:1135	During storage of the samples at 20 °C degradation of procyanidins was observed, whereas at -20 °C and -80 °C concentrations of them increased.
27145129	10	24	dep	CONCLUSION	1192:1201	arg1	have					1306:1309	have	1306:1309	have valuable biological activity, and strong antioxidant and anti-inflammatory properties	1306:1395	CONCLUSION The microcapsules with procyanidins from the bark of hawthorn, as well as the extract of procyanidins, have valuable biological activity, and strong antioxidant and anti-inflammatory properties.
27145129	8	25	theme	inulin	937:942	arg1	use					930:932	The use	926:932	The use of inulin during spray drying	926:962	The use of inulin during spray drying resulted in greater efficiency of micro-encapsulation than the use of maltodextrin.
27145129	11	26	theme	procyanidin/carrier	1479:1497	arg1	ratio					1499:1503	the procyanidin/carrier ratio 1:3	1475:1507	the procyanidin/carrier ratio 1:3	1475:1507	It is better to prepare microcapsules with a greater amount of carrier, with the procyanidin/carrier ratio 1:3.
27145129	10	27	theme	valuable	1311:1318	arg1	activity					1331:1338	valuable biological activity	1311:1338	valuable biological activity	1311:1338	CONCLUSION The microcapsules with procyanidins from the bark of hawthorn, as well as the extract of procyanidins, have valuable biological activity, and strong antioxidant and anti-inflammatory properties.
27145129	6	28	theme	ratio	615:619	arg1	effects					536:542	The effects	532:542	The effects of the carrier type (inulin and maltodextrin) and procyanidins:carrier ratio (1:1, 1:3)	532:630	The effects of the carrier type (inulin and maltodextrin) and procyanidins:carrier ratio (1:1, 1:3) and the influence of storage temperature (20 °C, -20 °C, -80 °C) on the content of procyanidins were evaluated.
27145129	6	28	theme	ratio	615:619	arg1	influence					640:648	the influence	636:648	the influence of storage temperature (20 °C, -20 °C, -80 °C) on the content of procyanidins	636:726	The effects of the carrier type (inulin and maltodextrin) and procyanidins:carrier ratio (1:1, 1:3) and the influence of storage temperature (20 °C, -20 °C, -80 °C) on the content of procyanidins were evaluated.
27145129	12	29	theme	Chemical	1528:1535	arg1	Industry					1537:1544	Chemical Industry	1528:1544	Chemical Industry	1528:1544	© 2016 Society of Chemical Industry.
27145129	7	30	theme	trimer	905:910	arg1	C1					912:913	trimer C1	905:913	trimer C1	905:913	RESULTS Samples before and after micro-encapsulation contained from 651 to 751 mg of procyanidins in 1 g. Among the procyanidins, (-)-epicatechin, dimer B2, and trimer C1 dominated.
27145129	6	31	theme	carrier	607:613	arg1	ratio					615:619	procyanidins:carrier ratio	594:619	procyanidins:carrier ratio (1:1, 1:3)	594:630	The effects of the carrier type (inulin and maltodextrin) and procyanidins:carrier ratio (1:1, 1:3) and the influence of storage temperature (20 °C, -20 °C, -80 °C) on the content of procyanidins were evaluated.
27145129	6	32	from	effects	536:542	arg1	content					704:710	the content	700:710	the content of procyanidins	700:726	The effects of the carrier type (inulin and maltodextrin) and procyanidins:carrier ratio (1:1, 1:3) and the influence of storage temperature (20 °C, -20 °C, -80 °C) on the content of procyanidins were evaluated.
27145129	10	33	from	bark	1248:1251	arg1	microcapsules					1207:1219	The microcapsules	1203:1219	The microcapsules with procyanidins from the bark of hawthorn, as well as the extract of procyanidins,	1203:1304	CONCLUSION The microcapsules with procyanidins from the bark of hawthorn, as well as the extract of procyanidins, have valuable biological activity, and strong antioxidant and anti-inflammatory properties.
27145129	8	34	theme	micro-encapsulation	998:1016	arg1	efficiency					984:993	greater efficiency	976:993	greater efficiency of micro-encapsulation than the use of maltodextrin	976:1045	The use of inulin during spray drying resulted in greater efficiency of micro-encapsulation than the use of maltodextrin.
27145129	4	35	theme	Qualitative	279:289	arg1	composition					308:318	Qualitative and quantitative composition	279:318	Qualitative and quantitative composition	279:318	Qualitative and quantitative composition was compared with that of the extract of hawthorn fruit (Crataegus monogyna Jacq.)
27145129	11	36	theme	carrier	1461:1467	arg1	carrier					1461:1467	carrier	1461:1467	carrier	1461:1467	It is better to prepare microcapsules with a greater amount of carrier, with the procyanidin/carrier ratio 1:3.
27145129	11	36	theme	carrier	1461:1467	arg1	amount					1451:1456	a greater amount	1441:1456	a greater amount of carrier	1441:1467	It is better to prepare microcapsules with a greater amount of carrier, with the procyanidin/carrier ratio 1:3.
27145129	6	37	from	influence	640:648	arg1	content					704:710	the content	700:710	the content of procyanidins	700:726	The effects of the carrier type (inulin and maltodextrin) and procyanidins:carrier ratio (1:1, 1:3) and the influence of storage temperature (20 °C, -20 °C, -80 °C) on the content of procyanidins were evaluated.
27145129	7	38	dep	751	819:821	arg1	to					816:817	to	816:817	to	816:817	RESULTS Samples before and after micro-encapsulation contained from 651 to 751 mg of procyanidins in 1 g. Among the procyanidins, (-)-epicatechin, dimer B2, and trimer C1 dominated.
27145129	6	39	theme	procyanidins	594:605	arg1	ratio					615:619	procyanidins:carrier ratio	594:619	procyanidins:carrier ratio (1:1, 1:3)	594:630	The effects of the carrier type (inulin and maltodextrin) and procyanidins:carrier ratio (1:1, 1:3) and the influence of storage temperature (20 °C, -20 °C, -80 °C) on the content of procyanidins were evaluated.
27145129	8	40	theme	maltodextrin	1034:1045	arg1	use					1027:1029	the use	1023:1029	the use of maltodextrin	1023:1045	The use of inulin during spray drying resulted in greater efficiency of micro-encapsulation than the use of maltodextrin.
27145129	2	41	theme	BACKGROUND	174:183	arg1	Jacq					244:247	Jacq	244:247	Jacq	244:247	BACKGROUND Procyanidins from the bark of hawthorn (Crataegus monogyna Jacq.)
27145129	2	41	theme	BACKGROUND	174:183	arg1	Procyanidins					185:196	BACKGROUND Procyanidins	174:196	BACKGROUND Procyanidins from the bark of hawthorn (Crataegus monogyna Jacq.)	174:249	BACKGROUND Procyanidins from the bark of hawthorn (Crataegus monogyna Jacq.)
27145129	6	42	theme	procyanidins	715:726	arg1	content					704:710	the content	700:710	the content of procyanidins	700:726	The effects of the carrier type (inulin and maltodextrin) and procyanidins:carrier ratio (1:1, 1:3) and the influence of storage temperature (20 °C, -20 °C, -80 °C) on the content of procyanidins were evaluated.
27145129	10	43	theme	procyanidins	1292:1303	arg1	hawthorn					1256:1263	hawthorn	1256:1263	hawthorn	1256:1263	CONCLUSION The microcapsules with procyanidins from the bark of hawthorn, as well as the extract of procyanidins, have valuable biological activity, and strong antioxidant and anti-inflammatory properties.
27145129	10	43	theme	procyanidins	1292:1303	arg1	extract					1281:1287	the extract	1277:1287	the extract of procyanidins	1277:1303	CONCLUSION The microcapsules with procyanidins from the bark of hawthorn, as well as the extract of procyanidins, have valuable biological activity, and strong antioxidant and anti-inflammatory properties.
27145129	6	44	dep	ratio	615:619	arg1	1:3					627:629	1:3	627:629	1:3	627:629	The effects of the carrier type (inulin and maltodextrin) and procyanidins:carrier ratio (1:1, 1:3) and the influence of storage temperature (20 °C, -20 °C, -80 °C) on the content of procyanidins were evaluated.
27145129	6	44	dep	ratio	615:619	arg1	1:1					622:624	1:1	622:624	1:1	622:624	The effects of the carrier type (inulin and maltodextrin) and procyanidins:carrier ratio (1:1, 1:3) and the influence of storage temperature (20 °C, -20 °C, -80 °C) on the content of procyanidins were evaluated.
27145129	4	45	theme	fruit	370:374	arg1	extract					350:356	the extract	346:356	the extract of hawthorn fruit	346:374	Qualitative and quantitative composition was compared with that of the extract of hawthorn fruit (Crataegus monogyna Jacq.)
27145129	6	46	theme	temperature	661:671	arg1	effects					536:542	The effects	532:542	The effects of the carrier type (inulin and maltodextrin) and procyanidins:carrier ratio (1:1, 1:3)	532:630	The effects of the carrier type (inulin and maltodextrin) and procyanidins:carrier ratio (1:1, 1:3) and the influence of storage temperature (20 °C, -20 °C, -80 °C) on the content of procyanidins were evaluated.
27145129	6	46	theme	temperature	661:671	arg1	influence					640:648	the influence	636:648	the influence of storage temperature (20 °C, -20 °C, -80 °C) on the content of procyanidins	636:726	The effects of the carrier type (inulin and maltodextrin) and procyanidins:carrier ratio (1:1, 1:3) and the influence of storage temperature (20 °C, -20 °C, -80 °C) on the content of procyanidins were evaluated.
27145129	2	47	theme	hawthorn	215:222	arg1	bark					207:210	the bark	203:210	the bark of hawthorn	203:222	BACKGROUND Procyanidins from the bark of hawthorn (Crataegus monogyna Jacq.)
27145129	11	48	theme	greater	1443:1449	arg1	carrier					1461:1467	carrier	1461:1467	carrier	1461:1467	It is better to prepare microcapsules with a greater amount of carrier, with the procyanidin/carrier ratio 1:3.
27145129	11	48	theme	greater	1443:1449	arg1	amount					1451:1456	a greater amount	1441:1456	a greater amount of carrier	1441:1467	It is better to prepare microcapsules with a greater amount of carrier, with the procyanidin/carrier ratio 1:3.
27145129	4	49	theme	hawthorn	361:368	arg1	fruit					370:374	hawthorn fruit	361:374	hawthorn fruit	361:374	Qualitative and quantitative composition was compared with that of the extract of hawthorn fruit (Crataegus monogyna Jacq.)
27145129	7	50	from	-epicatechin	877:888	arg1	mg					823:824	651 to 751 mg	812:824	651 to 751 mg of procyanidins in 1 g. Among the procyanidins, (-)-epicatechin, dimer B2, and trimer C1	812:913	RESULTS Samples before and after micro-encapsulation contained from 651 to 751 mg of procyanidins in 1 g. Among the procyanidins, (-)-epicatechin, dimer B2, and trimer C1 dominated.
27145129	5	51	theme	procyanidins	466:477	arg1	properties					452:461	antioxidant and anti-inflammatory properties	418:461	antioxidant and anti-inflammatory properties	418:461	Stability and antioxidant and anti-inflammatory properties of procyanidins before and after micro-encapsulation were estimated.
27145129	5	51	theme	procyanidins	466:477	arg1	Stability					404:412	Stability	404:412	Stability	404:412	Stability and antioxidant and anti-inflammatory properties of procyanidins before and after micro-encapsulation were estimated.
27145129	9	52	theme	samples	1070:1076	arg1	storage					1055:1061	storage	1055:1061	storage of the samples at 20 °C	1055:1085	During storage of the samples at 20 °C degradation of procyanidins was observed, whereas at -20 °C and -80 °C concentrations of them increased.
27145129	9	53	theme	them	1176:1179	arg1	concentrations					1158:1171	concentrations	1158:1171	concentrations of them	1158:1179	During storage of the samples at 20 °C degradation of procyanidins was observed, whereas at -20 °C and -80 °C concentrations of them increased.
27145129	7	54	theme	procyanidins	829:840	arg1	mg					823:824	651 to 751 mg	812:824	651 to 751 mg of procyanidins in 1 g. Among the procyanidins, (-)-epicatechin, dimer B2, and trimer C1	812:913	RESULTS Samples before and after micro-encapsulation contained from 651 to 751 mg of procyanidins in 1 g. Among the procyanidins, (-)-epicatechin, dimer B2, and trimer C1 dominated.
27145129	4	55	dep	compared	324:331	arg1	Jacq					396:399	Jacq	396:399	Jacq	396:399	Qualitative and quantitative composition was compared with that of the extract of hawthorn fruit (Crataegus monogyna Jacq.)
27145129	7	56	theme	g.	847:848	arg1	-epicatechin					877:888	1 g. Among the procyanidins, (-)-epicatechin	845:888	1 g. Among the procyanidins, (-)-epicatechin	845:888	RESULTS Samples before and after micro-encapsulation contained from 651 to 751 mg of procyanidins in 1 g. Among the procyanidins, (-)-epicatechin, dimer B2, and trimer C1 dominated.
27145129	6	57	theme	storage	653:659	arg1	temperature					661:671	storage temperature	653:671	storage temperature (20 °C, -20 °C, -80 °C)	653:695	The effects of the carrier type (inulin and maltodextrin) and procyanidins:carrier ratio (1:1, 1:3) and the influence of storage temperature (20 °C, -20 °C, -80 °C) on the content of procyanidins were evaluated.
27145129	7	58	from	procyanidins	829:840	arg1	-epicatechin					877:888	1 g. Among the procyanidins, (-)-epicatechin	845:888	1 g. Among the procyanidins, (-)-epicatechin	845:888	RESULTS Samples before and after micro-encapsulation contained from 651 to 751 mg of procyanidins in 1 g. Among the procyanidins, (-)-epicatechin, dimer B2, and trimer C1 dominated.
27145129	7	58	from	procyanidins	829:840	arg1	B2					897:898	dimer B2	891:898	dimer B2	891:898	RESULTS Samples before and after micro-encapsulation contained from 651 to 751 mg of procyanidins in 1 g. Among the procyanidins, (-)-epicatechin, dimer B2, and trimer C1 dominated.
27145129	7	58	from	procyanidins	829:840	arg1	C1					912:913	trimer C1	905:913	trimer C1	905:913	RESULTS Samples before and after micro-encapsulation contained from 651 to 751 mg of procyanidins in 1 g. Among the procyanidins, (-)-epicatechin, dimer B2, and trimer C1 dominated.
27145129	6	59	dep	temperature	661:671	arg1	°C					677:678	20 °C	674:678	20 °C	674:678	The effects of the carrier type (inulin and maltodextrin) and procyanidins:carrier ratio (1:1, 1:3) and the influence of storage temperature (20 °C, -20 °C, -80 °C) on the content of procyanidins were evaluated.
27145129	6	59	dep	temperature	661:671	arg1	°C					685:686	-20 °C	681:686	-20 °C	681:686	The effects of the carrier type (inulin and maltodextrin) and procyanidins:carrier ratio (1:1, 1:3) and the influence of storage temperature (20 °C, -20 °C, -80 °C) on the content of procyanidins were evaluated.
27145129	6	59	dep	temperature	661:671	arg1	°C					693:694	-80 °C	689:694	-80 °C	689:694	The effects of the carrier type (inulin and maltodextrin) and procyanidins:carrier ratio (1:1, 1:3) and the influence of storage temperature (20 °C, -20 °C, -80 °C) on the content of procyanidins were evaluated.
27145129	7	60	from	B2	897:898	arg1	mg					823:824	651 to 751 mg	812:824	651 to 751 mg of procyanidins in 1 g. Among the procyanidins, (-)-epicatechin, dimer B2, and trimer C1	812:913	RESULTS Samples before and after micro-encapsulation contained from 651 to 751 mg of procyanidins in 1 g. Among the procyanidins, (-)-epicatechin, dimer B2, and trimer C1 dominated.
27145129	5	61	theme	antioxidant	418:428	arg1	properties					452:461	antioxidant and anti-inflammatory properties	418:461	antioxidant and anti-inflammatory properties	418:461	Stability and antioxidant and anti-inflammatory properties of procyanidins before and after micro-encapsulation were estimated.
27145129	2	62	from	bark	207:210	arg1	Jacq					244:247	Jacq	244:247	Jacq	244:247	BACKGROUND Procyanidins from the bark of hawthorn (Crataegus monogyna Jacq.)
27145129	2	62	from	bark	207:210	arg1	Procyanidins					185:196	BACKGROUND Procyanidins	174:196	BACKGROUND Procyanidins from the bark of hawthorn (Crataegus monogyna Jacq.)	174:249	BACKGROUND Procyanidins from the bark of hawthorn (Crataegus monogyna Jacq.)
26572382	7	0	dep	suggesting	1099:1108	arg1	may					1095:1097	may	1095:1097	may	1095:1097	The kinetic adsorption models followed pseudo-second order model and the dye intra-particle diffusion may suggesting a chemical reaction mechanism.
26572382	8	1	theme	dye	1271:1273	arg1	removal					1275:1281	anionic dye removal	1263:1281	anionic dye removal from aqueous solutions	1263:1304	Further, it was obvious from the investigation that this composite could be applied as a promising low cost adsorbent for anionic dye removal from aqueous solutions.
26572382	5	2	theme	Experiment	706:715	arg1	results					717:723	Experiment results	706:723	Experiment results	706:723	Experiment results showed that the EC/Cs composite have high ability to remove C.I. Reactive Black 5 from its dye-bath effluent.
26572382	0	3	theme	5	67:67	arg1	adsorption					38:47	the adsorption	34:47	the adsorption of Reactive Black 5 from aqueous solution using Eichhornia crassipes/chitosan composite	34:135	Equilibrium and kinetic models on the adsorption of Reactive Black 5 from aqueous solution using Eichhornia crassipes/chitosan composite.
26572382	6	4	theme	isotherm	927:934	arg1	models					936:941	Langmuir adsorption isotherm models	907:941	Langmuir adsorption isotherm models	907:941	The equilibrium sorption evaluation of RB5 conformed and fitted well to Langmuir adsorption isotherm models and the maximum sorption capacity was 0.606 mg/g.
26572382	6	5	theme	equilibrium	839:849	arg1	evaluation					860:869	The equilibrium sorption evaluation	835:869	The equilibrium sorption evaluation of RB5	835:876	The equilibrium sorption evaluation of RB5 conformed and fitted well to Langmuir adsorption isotherm models and the maximum sorption capacity was 0.606 mg/g.
26572382	3	6	theme	micropore	540:548	arg1	volume					550:555	micropore volume	540:555	micropore volume	540:555	Surface characterization of EC/Cs natural biopolymer composite was examined by spectrum analysis FT-IR, specific surface area, micropore volume, pore width and SEM.
26572382	8	7	theme	anionic	1263:1269	arg1	removal					1275:1281	anionic dye removal	1263:1281	anionic dye removal from aqueous solutions	1263:1304	Further, it was obvious from the investigation that this composite could be applied as a promising low cost adsorbent for anionic dye removal from aqueous solutions.
26572382	4	8	theme	sorption	595:602	arg1	experiments					604:614	the sorption experiments	591:614	the sorption experiments	591:614	Furthermore, the sorption experiments were carried out as a function of pH, various initial dye concentration and contact time.
26572382	4	8	theme	sorption	595:602	arg1	function					638:645	a function	636:645	a function of pH, various initial dye concentration and contact time	636:703	Furthermore, the sorption experiments were carried out as a function of pH, various initial dye concentration and contact time.
26572382	0	9	from	Equilibrium	0:10	arg1	adsorption					38:47	the adsorption	34:47	the adsorption of Reactive Black 5 from aqueous solution using Eichhornia crassipes/chitosan composite	34:135	Equilibrium and kinetic models on the adsorption of Reactive Black 5 from aqueous solution using Eichhornia crassipes/chitosan composite.
26572382	7	10	theme	kinetic	997:1003	arg1	models					1016:1021	The kinetic adsorption models	993:1021	The kinetic adsorption models	993:1021	The kinetic adsorption models followed pseudo-second order model and the dye intra-particle diffusion may suggesting a chemical reaction mechanism.
26572382	0	11	theme	aqueous	74:80	arg1	solution					82:89	aqueous solution	74:89	aqueous solution using Eichhornia crassipes/chitosan composite	74:135	Equilibrium and kinetic models on the adsorption of Reactive Black 5 from aqueous solution using Eichhornia crassipes/chitosan composite.
26572382	6	12	theme	Langmuir	907:914	arg1	adsorption					916:925	Langmuir adsorption	907:925	Langmuir adsorption isotherm models	907:941	The equilibrium sorption evaluation of RB5 conformed and fitted well to Langmuir adsorption isotherm models and the maximum sorption capacity was 0.606 mg/g.
26572382	1	13	theme	New	138:140	arg1	composite					161:169	New natural biopolymer composite	138:169	New natural biopolymer composite	138:169	New natural biopolymer composite was prepared using extracted cellulose from an environmentally problematic water hyacinth Eichhornia crassipes (EC).
26572382	0	14	theme	Eichhornia	97:106	arg1	composite					127:135	Eichhornia crassipes/chitosan composite	97:135	Eichhornia crassipes/chitosan composite	97:135	Equilibrium and kinetic models on the adsorption of Reactive Black 5 from aqueous solution using Eichhornia crassipes/chitosan composite.
26572382	0	15	from	adsorption	38:47	arg1	solution					82:89	aqueous solution	74:89	aqueous solution using Eichhornia crassipes/chitosan composite	74:135	Equilibrium and kinetic models on the adsorption of Reactive Black 5 from aqueous solution using Eichhornia crassipes/chitosan composite.
26572382	2	16	theme	extracted	292:300	arg1	cellulose					302:310	The extracted cellulose	288:310	The extracted cellulose	288:310	The extracted cellulose was functionalized by chitosan and TiO2 nanoparticles to form EC/Chitosan (EC/Cs) composite network.
26572382	3	17	theme	biopolymer	455:464	arg1	composite					466:474	EC/Cs natural biopolymer composite	441:474	EC/Cs natural biopolymer composite	441:474	Surface characterization of EC/Cs natural biopolymer composite was examined by spectrum analysis FT-IR, specific surface area, micropore volume, pore width and SEM.
26572382	4	18	theme	dye	670:672	arg1	concentration					674:686	various initial dye concentration	654:686	various initial dye concentration	654:686	Furthermore, the sorption experiments were carried out as a function of pH, various initial dye concentration and contact time.
26572382	5	19	theme	high	762:765	arg1	ability					767:773	high ability	762:773	high ability to remove C.I. Reactive Black 5 from its dye-bath effluent	762:832	Experiment results showed that the EC/Cs composite have high ability to remove C.I. Reactive Black 5 from its dye-bath effluent.
26572382	5	20	theme	Reactive	790:797	arg1	C.I.					785:788	C.I. Reactive	785:797	C.I. Reactive Black 5	785:805	Experiment results showed that the EC/Cs composite have high ability to remove C.I. Reactive Black 5 from its dye-bath effluent.
26572382	5	21	theme	dye-bath	816:823	arg1	effluent					825:832	its dye-bath effluent	812:832	its dye-bath effluent	812:832	Experiment results showed that the EC/Cs composite have high ability to remove C.I. Reactive Black 5 from its dye-bath effluent.
26572382	4	22	theme	initial	662:668	arg1	concentration					674:686	various initial dye concentration	654:686	various initial dye concentration	654:686	Furthermore, the sorption experiments were carried out as a function of pH, various initial dye concentration and contact time.
26572382	7	23	theme	chemical	1112:1119	arg1	mechanism					1130:1138	a chemical reaction mechanism	1110:1138	a chemical reaction mechanism	1110:1138	The kinetic adsorption models followed pseudo-second order model and the dye intra-particle diffusion may suggesting a chemical reaction mechanism.
26572382	3	24	theme	pore	558:561	arg1	width					563:567	pore width	558:567	pore width	558:567	Surface characterization of EC/Cs natural biopolymer composite was examined by spectrum analysis FT-IR, specific surface area, micropore volume, pore width and SEM.
26572382	1	25	theme	extracted	190:198	arg1	cellulose					200:208	extracted cellulose	190:208	extracted cellulose	190:208	New natural biopolymer composite was prepared using extracted cellulose from an environmentally problematic water hyacinth Eichhornia crassipes (EC).
26572382	3	26	theme	Surface	413:419	arg1	characterization					421:436	Surface characterization	413:436	Surface characterization of EC/Cs natural biopolymer composite	413:474	Surface characterization of EC/Cs natural biopolymer composite was examined by spectrum analysis FT-IR, specific surface area, micropore volume, pore width and SEM.
26572382	5	27	dep	Black	799:803	arg1	5					805:805	5	805:805	5	805:805	Experiment results showed that the EC/Cs composite have high ability to remove C.I. Reactive Black 5 from its dye-bath effluent.
26572382	2	28	theme	composite	394:402	arg1	network					404:410	EC/Chitosan (EC/Cs) composite network	374:410	EC/Chitosan (EC/Cs) composite network	374:410	The extracted cellulose was functionalized by chitosan and TiO2 nanoparticles to form EC/Chitosan (EC/Cs) composite network.
26572382	8	29	theme	low	1240:1242	arg1	composite					1198:1206	this composite	1193:1206	this composite	1193:1206	Further, it was obvious from the investigation that this composite could be applied as a promising low cost adsorbent for anionic dye removal from aqueous solutions.
26572382	8	29	theme	low	1240:1242	arg1	cost					1244:1247	a promising low cost	1228:1247	a promising low cost adsorbent for anionic dye removal from aqueous solutions	1228:1304	Further, it was obvious from the investigation that this composite could be applied as a promising low cost adsorbent for anionic dye removal from aqueous solutions.
26572382	4	30	theme	pH	650:651	arg1	experiments					604:614	the sorption experiments	591:614	the sorption experiments	591:614	Furthermore, the sorption experiments were carried out as a function of pH, various initial dye concentration and contact time.
26572382	4	30	theme	pH	650:651	arg1	function					638:645	a function	636:645	a function of pH, various initial dye concentration and contact time	636:703	Furthermore, the sorption experiments were carried out as a function of pH, various initial dye concentration and contact time.
26572382	0	31	theme	kinetic	16:22	arg1	models					24:29	kinetic models	16:29	kinetic models	16:29	Equilibrium and kinetic models on the adsorption of Reactive Black 5 from aqueous solution using Eichhornia crassipes/chitosan composite.
26572382	0	32	theme	crassipes/chitosan	108:125	arg1	composite					127:135	Eichhornia crassipes/chitosan composite	97:135	Eichhornia crassipes/chitosan composite	97:135	Equilibrium and kinetic models on the adsorption of Reactive Black 5 from aqueous solution using Eichhornia crassipes/chitosan composite.
26572382	7	33	theme	pseudo-second	1032:1044	arg1	model					1052:1056	pseudo-second order model	1032:1056	pseudo-second order model	1032:1056	The kinetic adsorption models followed pseudo-second order model and the dye intra-particle diffusion may suggesting a chemical reaction mechanism.
26572382	8	34	from	solutions	1296:1304	arg1	removal					1275:1281	anionic dye removal	1263:1281	anionic dye removal from aqueous solutions	1263:1304	Further, it was obvious from the investigation that this composite could be applied as a promising low cost adsorbent for anionic dye removal from aqueous solutions.
26572382	4	35	theme	time	700:703	arg1	experiments					604:614	the sorption experiments	591:614	the sorption experiments	591:614	Furthermore, the sorption experiments were carried out as a function of pH, various initial dye concentration and contact time.
26572382	4	35	theme	time	700:703	arg1	function					638:645	a function	636:645	a function of pH, various initial dye concentration and contact time	636:703	Furthermore, the sorption experiments were carried out as a function of pH, various initial dye concentration and contact time.
26572382	3	36	theme	EC/Cs	441:445	arg1	composite					466:474	EC/Cs natural biopolymer composite	441:474	EC/Cs natural biopolymer composite	441:474	Surface characterization of EC/Cs natural biopolymer composite was examined by spectrum analysis FT-IR, specific surface area, micropore volume, pore width and SEM.
26572382	3	37	theme	composite	466:474	arg1	characterization					421:436	Surface characterization	413:436	Surface characterization of EC/Cs natural biopolymer composite	413:474	Surface characterization of EC/Cs natural biopolymer composite was examined by spectrum analysis FT-IR, specific surface area, micropore volume, pore width and SEM.
26572382	4	38	theme	various	654:660	arg1	concentration					674:686	various initial dye concentration	654:686	various initial dye concentration	654:686	Furthermore, the sorption experiments were carried out as a function of pH, various initial dye concentration and contact time.
26572382	3	39	theme	natural	447:453	arg1	composite					466:474	EC/Cs natural biopolymer composite	441:474	EC/Cs natural biopolymer composite	441:474	Surface characterization of EC/Cs natural biopolymer composite was examined by spectrum analysis FT-IR, specific surface area, micropore volume, pore width and SEM.
26572382	5	40	theme	Black	799:803	arg1	C.I.					785:788	C.I. Reactive	785:797	C.I. Reactive Black 5	785:805	Experiment results showed that the EC/Cs composite have high ability to remove C.I. Reactive Black 5 from its dye-bath effluent.
26572382	6	41	theme	RB5	874:876	arg1	evaluation					860:869	The equilibrium sorption evaluation	835:869	The equilibrium sorption evaluation of RB5	835:876	The equilibrium sorption evaluation of RB5 conformed and fitted well to Langmuir adsorption isotherm models and the maximum sorption capacity was 0.606 mg/g.
26572382	7	42	theme	order	1046:1050	arg1	model					1052:1056	pseudo-second order model	1032:1056	pseudo-second order model	1032:1056	The kinetic adsorption models followed pseudo-second order model and the dye intra-particle diffusion may suggesting a chemical reaction mechanism.
26572382	3	43	theme	spectrum	492:499	arg1	analysis					501:508	spectrum analysis FT-IR, specific surface area, micropore volume, pore width and SEM	492:575	spectrum analysis FT-IR, specific surface area, micropore volume, pore width and SEM	492:575	Surface characterization of EC/Cs natural biopolymer composite was examined by spectrum analysis FT-IR, specific surface area, micropore volume, pore width and SEM.
26572382	8	44	theme	adsorbent	1249:1257	arg1	composite					1198:1206	this composite	1193:1206	this composite	1193:1206	Further, it was obvious from the investigation that this composite could be applied as a promising low cost adsorbent for anionic dye removal from aqueous solutions.
26572382	8	44	theme	adsorbent	1249:1257	arg1	cost					1244:1247	a promising low cost	1228:1247	a promising low cost adsorbent for anionic dye removal from aqueous solutions	1228:1304	Further, it was obvious from the investigation that this composite could be applied as a promising low cost adsorbent for anionic dye removal from aqueous solutions.
26572382	6	45	theme	sorption	959:966	arg1	capacity					968:975	the maximum sorption capacity	947:975	the maximum sorption capacity	947:975	The equilibrium sorption evaluation of RB5 conformed and fitted well to Langmuir adsorption isotherm models and the maximum sorption capacity was 0.606 mg/g.
26572382	6	45	theme	sorption	959:966	arg1	mg/g					987:990	0.606 mg/g	981:990	0.606 mg/g	981:990	The equilibrium sorption evaluation of RB5 conformed and fitted well to Langmuir adsorption isotherm models and the maximum sorption capacity was 0.606 mg/g.
26572382	7	46	theme	dye	1066:1068	arg1	diffusion					1085:1093	the dye intra-particle diffusion	1062:1093	the dye intra-particle diffusion	1062:1093	The kinetic adsorption models followed pseudo-second order model and the dye intra-particle diffusion may suggesting a chemical reaction mechanism.
26572382	8	47	from	investigation	1174:1186	arg1	obvious					1157:1163	obvious	1157:1163	obvious	1157:1163	Further, it was obvious from the investigation that this composite could be applied as a promising low cost adsorbent for anionic dye removal from aqueous solutions.
26572382	3	48	theme	surface	526:532	arg1	area					534:537	specific surface area	517:537	specific surface area	517:537	Surface characterization of EC/Cs natural biopolymer composite was examined by spectrum analysis FT-IR, specific surface area, micropore volume, pore width and SEM.
26572382	6	49	theme	adsorption	916:925	arg1	models					936:941	Langmuir adsorption isotherm models	907:941	Langmuir adsorption isotherm models	907:941	The equilibrium sorption evaluation of RB5 conformed and fitted well to Langmuir adsorption isotherm models and the maximum sorption capacity was 0.606 mg/g.
26572382	6	50	theme	maximum	951:957	arg1	capacity					968:975	the maximum sorption capacity	947:975	the maximum sorption capacity	947:975	The equilibrium sorption evaluation of RB5 conformed and fitted well to Langmuir adsorption isotherm models and the maximum sorption capacity was 0.606 mg/g.
26572382	6	50	theme	maximum	951:957	arg1	mg/g					987:990	0.606 mg/g	981:990	0.606 mg/g	981:990	The equilibrium sorption evaluation of RB5 conformed and fitted well to Langmuir adsorption isotherm models and the maximum sorption capacity was 0.606 mg/g.
26572382	7	51	theme	intra-particle	1070:1083	arg1	diffusion					1085:1093	the dye intra-particle diffusion	1062:1093	the dye intra-particle diffusion	1062:1093	The kinetic adsorption models followed pseudo-second order model and the dye intra-particle diffusion may suggesting a chemical reaction mechanism.
26572382	5	52	contain	have	757:760	arg1	EC/Cs					741:745	the EC/Cs	737:745	the EC/Cs composite	737:755	Experiment results showed that the EC/Cs composite have high ability to remove C.I. Reactive Black 5 from its dye-bath effluent.
26572382	5	52	contain	have	757:760	arg2	ability					767:773	high ability	762:773	high ability to remove C.I. Reactive Black 5 from its dye-bath effluent	762:832	Experiment results showed that the EC/Cs composite have high ability to remove C.I. Reactive Black 5 from its dye-bath effluent.
26572382	8	53	theme	promising	1230:1238	arg1	composite					1198:1206	this composite	1193:1206	this composite	1193:1206	Further, it was obvious from the investigation that this composite could be applied as a promising low cost adsorbent for anionic dye removal from aqueous solutions.
26572382	8	53	theme	promising	1230:1238	arg1	cost					1244:1247	a promising low cost	1228:1247	a promising low cost adsorbent for anionic dye removal from aqueous solutions	1228:1304	Further, it was obvious from the investigation that this composite could be applied as a promising low cost adsorbent for anionic dye removal from aqueous solutions.
26572382	4	54	theme	concentration	674:686	arg1	experiments					604:614	the sorption experiments	591:614	the sorption experiments	591:614	Furthermore, the sorption experiments were carried out as a function of pH, various initial dye concentration and contact time.
26572382	4	54	theme	concentration	674:686	arg1	function					638:645	a function	636:645	a function of pH, various initial dye concentration and contact time	636:703	Furthermore, the sorption experiments were carried out as a function of pH, various initial dye concentration and contact time.
26572382	6	55	theme	sorption	851:858	arg1	evaluation					860:869	The equilibrium sorption evaluation	835:869	The equilibrium sorption evaluation of RB5	835:876	The equilibrium sorption evaluation of RB5 conformed and fitted well to Langmuir adsorption isotherm models and the maximum sorption capacity was 0.606 mg/g.
26572382	4	56	theme	contact	692:698	arg1	time					700:703	contact time	692:703	contact time	692:703	Furthermore, the sorption experiments were carried out as a function of pH, various initial dye concentration and contact time.
26572382	1	57	theme	water	246:250	arg1	EC					283:284	EC	283:284	EC	283:284	New natural biopolymer composite was prepared using extracted cellulose from an environmentally problematic water hyacinth Eichhornia crassipes (EC).
26572382	1	57	theme	water	246:250	arg1	crassipes					272:280	an environmentally problematic water hyacinth Eichhornia crassipes	215:280	an environmentally problematic water hyacinth Eichhornia crassipes (EC)	215:285	New natural biopolymer composite was prepared using extracted cellulose from an environmentally problematic water hyacinth Eichhornia crassipes (EC).
26572382	5	58	attach	remove	778:783	arg1	effluent					825:832	its dye-bath effluent	812:832	its dye-bath effluent	812:832	Experiment results showed that the EC/Cs composite have high ability to remove C.I. Reactive Black 5 from its dye-bath effluent.
26572382	5	58	attach	remove	778:783	arg2	ability					767:773	high ability	762:773	high ability to remove C.I. Reactive Black 5 from its dye-bath effluent	762:832	Experiment results showed that the EC/Cs composite have high ability to remove C.I. Reactive Black 5 from its dye-bath effluent.
26572382	0	59	from	solution	82:89	arg1	adsorption					38:47	the adsorption	34:47	the adsorption of Reactive Black 5 from aqueous solution using Eichhornia crassipes/chitosan composite	34:135	Equilibrium and kinetic models on the adsorption of Reactive Black 5 from aqueous solution using Eichhornia crassipes/chitosan composite.
26572382	7	60	theme	adsorption	1005:1014	arg1	models					1016:1021	The kinetic adsorption models	993:1021	The kinetic adsorption models	993:1021	The kinetic adsorption models followed pseudo-second order model and the dye intra-particle diffusion may suggesting a chemical reaction mechanism.
26572382	2	61	dep	chitosan	334:341	arg1	nanoparticles					352:364	nanoparticles	352:364	nanoparticles	352:364	The extracted cellulose was functionalized by chitosan and TiO2 nanoparticles to form EC/Chitosan (EC/Cs) composite network.
26572382	7	62	theme	reaction	1121:1128	arg1	mechanism					1130:1138	a chemical reaction mechanism	1110:1138	a chemical reaction mechanism	1110:1138	The kinetic adsorption models followed pseudo-second order model and the dye intra-particle diffusion may suggesting a chemical reaction mechanism.
26572382	1	63	theme	problematic	234:244	arg1	EC					283:284	EC	283:284	EC	283:284	New natural biopolymer composite was prepared using extracted cellulose from an environmentally problematic water hyacinth Eichhornia crassipes (EC).
26572382	1	63	theme	problematic	234:244	arg1	crassipes					272:280	an environmentally problematic water hyacinth Eichhornia crassipes	215:280	an environmentally problematic water hyacinth Eichhornia crassipes (EC)	215:285	New natural biopolymer composite was prepared using extracted cellulose from an environmentally problematic water hyacinth Eichhornia crassipes (EC).
26572382	3	64	dep	analysis	501:508	arg1	SEM					573:575	SEM	573:575	SEM	573:575	Surface characterization of EC/Cs natural biopolymer composite was examined by spectrum analysis FT-IR, specific surface area, micropore volume, pore width and SEM.
26572382	3	64	dep	analysis	501:508	arg1	area					534:537	specific surface area	517:537	specific surface area	517:537	Surface characterization of EC/Cs natural biopolymer composite was examined by spectrum analysis FT-IR, specific surface area, micropore volume, pore width and SEM.
26572382	3	64	dep	analysis	501:508	arg1	volume					550:555	micropore volume	540:555	micropore volume	540:555	Surface characterization of EC/Cs natural biopolymer composite was examined by spectrum analysis FT-IR, specific surface area, micropore volume, pore width and SEM.
26572382	3	64	dep	analysis	501:508	arg1	width					563:567	pore width	558:567	pore width	558:567	Surface characterization of EC/Cs natural biopolymer composite was examined by spectrum analysis FT-IR, specific surface area, micropore volume, pore width and SEM.
26572382	3	64	dep	analysis	501:508	arg1	FT-IR					510:514	FT-IR	510:514	FT-IR	510:514	Surface characterization of EC/Cs natural biopolymer composite was examined by spectrum analysis FT-IR, specific surface area, micropore volume, pore width and SEM.
26572382	8	65	theme	aqueous	1288:1294	arg1	solutions					1296:1304	aqueous solutions	1288:1304	aqueous solutions	1288:1304	Further, it was obvious from the investigation that this composite could be applied as a promising low cost adsorbent for anionic dye removal from aqueous solutions.
26572382	1	66	theme	natural	142:148	arg1	composite					161:169	New natural biopolymer composite	138:169	New natural biopolymer composite	138:169	New natural biopolymer composite was prepared using extracted cellulose from an environmentally problematic water hyacinth Eichhornia crassipes (EC).
26572382	3	67	theme	specific	517:524	arg1	area					534:537	specific surface area	517:537	specific surface area	517:537	Surface characterization of EC/Cs natural biopolymer composite was examined by spectrum analysis FT-IR, specific surface area, micropore volume, pore width and SEM.
26572382	1	68	theme	Eichhornia	261:270	arg1	EC					283:284	EC	283:284	EC	283:284	New natural biopolymer composite was prepared using extracted cellulose from an environmentally problematic water hyacinth Eichhornia crassipes (EC).
26572382	1	68	theme	Eichhornia	261:270	arg1	crassipes					272:280	an environmentally problematic water hyacinth Eichhornia crassipes	215:280	an environmentally problematic water hyacinth Eichhornia crassipes (EC)	215:285	New natural biopolymer composite was prepared using extracted cellulose from an environmentally problematic water hyacinth Eichhornia crassipes (EC).
26572382	0	69	from	models	24:29	arg1	adsorption					38:47	the adsorption	34:47	the adsorption of Reactive Black 5 from aqueous solution using Eichhornia crassipes/chitosan composite	34:135	Equilibrium and kinetic models on the adsorption of Reactive Black 5 from aqueous solution using Eichhornia crassipes/chitosan composite.
26572382	1	70	theme	biopolymer	150:159	arg1	composite					161:169	New natural biopolymer composite	138:169	New natural biopolymer composite	138:169	New natural biopolymer composite was prepared using extracted cellulose from an environmentally problematic water hyacinth Eichhornia crassipes (EC).
26572382	5	71	theme	composite	747:755	arg1	EC/Cs					741:745	the EC/Cs	737:745	the EC/Cs composite	737:755	Experiment results showed that the EC/Cs composite have high ability to remove C.I. Reactive Black 5 from its dye-bath effluent.
26537466	5	0	theme	biomechanical	823:835	arg1	properties					837:846	the biochemical and biomechanical properties	803:846	the biochemical and biomechanical properties of proteoglycans	803:863	Proteoglycan mimics like the ones proposed here could be used to study the structure-property relationships of proteoglycans and to introduce the biochemical and biomechanical properties of proteoglycans into biomaterials and therapeutic formulations.
26537466	4	1	theme	reductive	560:568	arg1	amination					570:578	reductive amination	560:578	reductive amination to couple the glycosaminoglycan side chain to the backbone	560:637	The method presented here uses a three-step reaction mechanism to first functionalize a hyaluronic acid backbone, followed by reductive amination to couple the glycosaminoglycan side chain to the backbone, by the reducing end.
26537466	4	2	theme	reaction	478:485	arg1	mechanism					487:495	a three-step reaction mechanism	465:495	a three-step reaction mechanism	465:495	The method presented here uses a three-step reaction mechanism to first functionalize a hyaluronic acid backbone, followed by reductive amination to couple the glycosaminoglycan side chain to the backbone, by the reducing end.
26537466	2	3	with	preparation	178:188	arg1	chains					238:243	glycosaminoglycan side chains	215:243	glycosaminoglycan side chains	215:243	The method described here enables the preparation of graft copolymers with glycosaminoglycan side chains, which mimic the structure and composition of proteoglycans.
26537466	5	4	theme	proteoglycans	772:784	arg1	relationships					755:767	the structure-property relationships	732:767	the structure-property relationships of proteoglycans	732:784	Proteoglycan mimics like the ones proposed here could be used to study the structure-property relationships of proteoglycans and to introduce the biochemical and biomechanical properties of proteoglycans into biomaterials and therapeutic formulations.
26537466	4	5	dep	amination	570:578	arg1	couple					583:588	couple	583:588	to couple the glycosaminoglycan side chain to the backbone	580:637	The method presented here uses a three-step reaction mechanism to first functionalize a hyaluronic acid backbone, followed by reductive amination to couple the glycosaminoglycan side chain to the backbone, by the reducing end.
26537466	4	6	theme	hyaluronic	522:531	arg1	backbone					538:545	a hyaluronic acid backbone	520:545	a hyaluronic acid backbone	520:545	The method presented here uses a three-step reaction mechanism to first functionalize a hyaluronic acid backbone, followed by reductive amination to couple the glycosaminoglycan side chain to the backbone, by the reducing end.
26537466	5	7	theme	Proteoglycan	661:672	arg1	mimics					674:679	Proteoglycan mimics	661:679	Proteoglycan mimics like the ones proposed here	661:707	Proteoglycan mimics like the ones proposed here could be used to study the structure-property relationships of proteoglycans and to introduce the biochemical and biomechanical properties of proteoglycans into biomaterials and therapeutic formulations.
26537466	3	8	theme	wide	380:383	arg1	range					385:389	a wide range	378:389	a wide range of glycosaminoglycan side-chain densities	378:431	By controlling the stoichiometry, graft copolymers can be obtained with a wide range of glycosaminoglycan side-chain densities.
26537466	1	9	theme	pendant	86:92	arg1	chains					132:137	pendant glycosaminoglycan polysaccharide side chains	86:137	pendant glycosaminoglycan polysaccharide side chains	86:137	Proteoglycans are proteins with pendant glycosaminoglycan polysaccharide side chains.
26537466	3	10	theme	densities	423:431	arg1	range					385:389	a wide range	378:389	a wide range of glycosaminoglycan side-chain densities	378:431	By controlling the stoichiometry, graft copolymers can be obtained with a wide range of glycosaminoglycan side-chain densities.
26537466	4	11	theme	acid	533:536	arg1	backbone					538:545	a hyaluronic acid backbone	520:545	a hyaluronic acid backbone	520:545	The method presented here uses a three-step reaction mechanism to first functionalize a hyaluronic acid backbone, followed by reductive amination to couple the glycosaminoglycan side chain to the backbone, by the reducing end.
26537466	1	12	theme	glycosaminoglycan	94:110	arg1	chains					132:137	pendant glycosaminoglycan polysaccharide side chains	86:137	pendant glycosaminoglycan polysaccharide side chains	86:137	Proteoglycans are proteins with pendant glycosaminoglycan polysaccharide side chains.
26537466	4	13	theme	side	612:615	arg1	chain					617:621	the glycosaminoglycan side chain	590:621	the glycosaminoglycan side chain	590:621	The method presented here uses a three-step reaction mechanism to first functionalize a hyaluronic acid backbone, followed by reductive amination to couple the glycosaminoglycan side chain to the backbone, by the reducing end.
26537466	4	14	theme	three-step	467:476	arg1	mechanism					487:495	a three-step reaction mechanism	465:495	a three-step reaction mechanism	465:495	The method presented here uses a three-step reaction mechanism to first functionalize a hyaluronic acid backbone, followed by reductive amination to couple the glycosaminoglycan side chain to the backbone, by the reducing end.
26537466	1	15	theme	polysaccharide	112:125	arg1	chains					132:137	pendant glycosaminoglycan polysaccharide side chains	86:137	pendant glycosaminoglycan polysaccharide side chains	86:137	Proteoglycans are proteins with pendant glycosaminoglycan polysaccharide side chains.
26537466	5	16	used	used	718:721	arg2	mimics					674:679	Proteoglycan mimics	661:679	Proteoglycan mimics like the ones proposed here	661:707	Proteoglycan mimics like the ones proposed here could be used to study the structure-property relationships of proteoglycans and to introduce the biochemical and biomechanical properties of proteoglycans into biomaterials and therapeutic formulations.
26537466	3	17	theme	glycosaminoglycan	394:410	arg1	densities					423:431	glycosaminoglycan side-chain densities	394:431	glycosaminoglycan side-chain densities	394:431	By controlling the stoichiometry, graft copolymers can be obtained with a wide range of glycosaminoglycan side-chain densities.
26537466	4	18	theme	glycosaminoglycan	594:610	arg1	chain					617:621	the glycosaminoglycan side chain	590:621	the glycosaminoglycan side chain	590:621	The method presented here uses a three-step reaction mechanism to first functionalize a hyaluronic acid backbone, followed by reductive amination to couple the glycosaminoglycan side chain to the backbone, by the reducing end.
26537466	1	19	theme	side	127:130	arg1	chains					132:137	pendant glycosaminoglycan polysaccharide side chains	86:137	pendant glycosaminoglycan polysaccharide side chains	86:137	Proteoglycans are proteins with pendant glycosaminoglycan polysaccharide side chains.
26537466	3	20	theme	side-chain	412:421	arg1	densities					423:431	glycosaminoglycan side-chain densities	394:431	glycosaminoglycan side-chain densities	394:431	By controlling the stoichiometry, graft copolymers can be obtained with a wide range of glycosaminoglycan side-chain densities.
26537466	5	21	theme	structure-property	736:753	arg1	relationships					755:767	the structure-property relationships	732:767	the structure-property relationships of proteoglycans	732:784	Proteoglycan mimics like the ones proposed here could be used to study the structure-property relationships of proteoglycans and to introduce the biochemical and biomechanical properties of proteoglycans into biomaterials and therapeutic formulations.
26537466	0	22	theme	Copolymers	42:51	arg1	Preparation					0:10	Preparation	0:10	Preparation of Proteoglycan Mimetic Graft Copolymers.	0:52	Preparation of Proteoglycan Mimetic Graft Copolymers.
26537466	2	23	theme	copolymers	199:208	arg1	preparation					178:188	the preparation	174:188	the preparation of graft copolymers with glycosaminoglycan side chains, which mimic the structure and composition of proteoglycans	174:303	The method described here enables the preparation of graft copolymers with glycosaminoglycan side chains, which mimic the structure and composition of proteoglycans.
26537466	2	24	dep	structure	262:270	arg1	the					258:260	the	258:260	the	258:260	The method described here enables the preparation of graft copolymers with glycosaminoglycan side chains, which mimic the structure and composition of proteoglycans.
26537466	0	25	theme	Graft	36:40	arg1	Copolymers					42:51	Proteoglycan Mimetic Graft Copolymers	15:51	Proteoglycan Mimetic Graft Copolymers	15:51	Preparation of Proteoglycan Mimetic Graft Copolymers.
26537466	2	26	theme	graft	193:197	arg1	copolymers					199:208	graft copolymers	193:208	graft copolymers	193:208	The method described here enables the preparation of graft copolymers with glycosaminoglycan side chains, which mimic the structure and composition of proteoglycans.
26537466	0	27	theme	Mimetic	28:34	arg1	Copolymers					42:51	Proteoglycan Mimetic Graft Copolymers	15:51	Proteoglycan Mimetic Graft Copolymers	15:51	Preparation of Proteoglycan Mimetic Graft Copolymers.
26537466	5	28	theme	therapeutic	887:897	arg1	formulations					899:910	therapeutic formulations	887:910	therapeutic formulations	887:910	Proteoglycan mimics like the ones proposed here could be used to study the structure-property relationships of proteoglycans and to introduce the biochemical and biomechanical properties of proteoglycans into biomaterials and therapeutic formulations.
26537466	5	29	theme	proteoglycans	851:863	arg1	properties					837:846	the biochemical and biomechanical properties	803:846	the biochemical and biomechanical properties of proteoglycans	803:863	Proteoglycan mimics like the ones proposed here could be used to study the structure-property relationships of proteoglycans and to introduce the biochemical and biomechanical properties of proteoglycans into biomaterials and therapeutic formulations.
26537466	3	30	theme	graft	340:344	arg1	copolymers					346:355	graft copolymers	340:355	graft copolymers	340:355	By controlling the stoichiometry, graft copolymers can be obtained with a wide range of glycosaminoglycan side-chain densities.
26537466	1	31	with	proteins	72:79	arg1	chains					132:137	pendant glycosaminoglycan polysaccharide side chains	86:137	pendant glycosaminoglycan polysaccharide side chains	86:137	Proteoglycans are proteins with pendant glycosaminoglycan polysaccharide side chains.
26537466	2	32	theme	proteoglycans	291:303	arg1	composition					276:286	composition	276:286	composition	276:286	The method described here enables the preparation of graft copolymers with glycosaminoglycan side chains, which mimic the structure and composition of proteoglycans.
26537466	2	32	theme	proteoglycans	291:303	arg1	structure					262:270	structure	262:270	structure	262:270	The method described here enables the preparation of graft copolymers with glycosaminoglycan side chains, which mimic the structure and composition of proteoglycans.
26537466	4	33	theme	reducing	647:654	arg1	end					656:658	the reducing end	643:658	the reducing end	643:658	The method presented here uses a three-step reaction mechanism to first functionalize a hyaluronic acid backbone, followed by reductive amination to couple the glycosaminoglycan side chain to the backbone, by the reducing end.
26537466	2	34	theme	side	233:236	arg1	chains					238:243	glycosaminoglycan side chains	215:243	glycosaminoglycan side chains	215:243	The method described here enables the preparation of graft copolymers with glycosaminoglycan side chains, which mimic the structure and composition of proteoglycans.
26537466	2	35	theme	glycosaminoglycan	215:231	arg1	chains					238:243	glycosaminoglycan side chains	215:243	glycosaminoglycan side chains	215:243	The method described here enables the preparation of graft copolymers with glycosaminoglycan side chains, which mimic the structure and composition of proteoglycans.
26537466	5	36	theme	biochemical	807:817	arg1	properties					837:846	the biochemical and biomechanical properties	803:846	the biochemical and biomechanical properties of proteoglycans	803:863	Proteoglycan mimics like the ones proposed here could be used to study the structure-property relationships of proteoglycans and to introduce the biochemical and biomechanical properties of proteoglycans into biomaterials and therapeutic formulations.
26537466	0	37	theme	Proteoglycan	15:26	arg1	Copolymers					42:51	Proteoglycan Mimetic Graft Copolymers	15:51	Proteoglycan Mimetic Graft Copolymers	15:51	Preparation of Proteoglycan Mimetic Graft Copolymers.
27314146	0	0	theme	Confluent	49:57	arg1	Matrices					59:66	Carboxylated Agarose (CA)-Silk Fibroin (SF) Dual Confluent Matrices	0:66	Carboxylated Agarose (CA)-Silk Fibroin (SF) Dual Confluent Matrices	0:66	Carboxylated Agarose (CA)-Silk Fibroin (SF) Dual Confluent Matrices Containing Oriented Hydroxyapatite (HA) Crystals: Biomimetic Organic/Inorganic Composites for Tibia Repair.
27314146	4	1	theme	optimal	914:920	arg1	properties					933:942	optimal mechanical properties	914:942	optimal mechanical properties that could meet the standard of bone repair	914:986	In the presence of SF, the composites showed optimal mechanical properties that could meet the standard of bone repair.
27314146	0	2	theme	Tibia	162:166	arg1	Repair					168:173	Tibia Repair	162:173	Tibia Repair	162:173	Carboxylated Agarose (CA)-Silk Fibroin (SF) Dual Confluent Matrices Containing Oriented Hydroxyapatite (HA) Crystals: Biomimetic Organic/Inorganic Composites for Tibia Repair.
27314146	1	3	theme	load-bearing	471:482	arg1	repair					489:494	load-bearing bone repair	471:494	load-bearing bone repair	471:494	By in situ combining the dual cross-linking matrices of the carboxylated agarose (CA) and the silk fibroin (SF) with the hydroxyapatite (HA) crystals, the CA-SF/HA composites with optimal physicochemical and biological properties were obtained, which were designed to meet the clinical needs of load-bearing bone repair.
27314146	3	4	theme	higher	831:836	arg1	stability					846:854	higher thermal stability	831:854	higher thermal stability	831:854	The chemical reactivity of the native agarose (NA) was significantly enhanced via carboxylation, and the CA exhibited higher thermal stability than the NA.
27314146	7	5	theme	formed	1464:1469	arg1	bone					1471:1474	newly formed bone	1458:1474	newly formed bone	1458:1474	After 24 days of implantation, newly formed bone was observed at the tibia defect site and around the implant.
27314146	0	6	theme	Dual	44:47	arg1	Matrices					59:66	Carboxylated Agarose (CA)-Silk Fibroin (SF) Dual Confluent Matrices	0:66	Carboxylated Agarose (CA)-Silk Fibroin (SF) Dual Confluent Matrices	0:66	Carboxylated Agarose (CA)-Silk Fibroin (SF) Dual Confluent Matrices Containing Oriented Hydroxyapatite (HA) Crystals: Biomimetic Organic/Inorganic Composites for Tibia Repair.
27314146	5	7	theme	SF	1049:1050	arg1	presence					1030:1037	the presence	1026:1037	the presence of CA and SF	1026:1050	The degradation of the composites in the presence of CA and SF was significantly delayed such that the degradation rate of the implant could satisfy the growth rate of the newly formed bone tissue.
27314146	2	8	theme	organic	541:547	arg1	matrices					549:556	the dual organic matrices	532:556	the dual organic matrices	532:556	With the synergistic modulation of the dual organic matrices, the HA nanoparticles presented sheet and rod morphologies due to the preferred orientation, which successfully simulated the biomineralization in nature.
27314146	2	9	theme	rod	600:602	arg1	morphologies					604:615	sheet and rod morphologies	590:615	sheet and rod morphologies	590:615	With the synergistic modulation of the dual organic matrices, the HA nanoparticles presented sheet and rod morphologies due to the preferred orientation, which successfully simulated the biomineralization in nature.
27314146	5	10	theme	composites	1012:1021	arg1	degradation					993:1003	The degradation	989:1003	The degradation of the composites in the presence of CA and SF	989:1050	The degradation of the composites in the presence of CA and SF was significantly delayed such that the degradation rate of the implant could satisfy the growth rate of the newly formed bone tissue.
27314146	5	11	theme	formed	1167:1172	arg1	tissue					1179:1184	the newly formed bone tissue	1157:1184	the newly formed bone tissue	1157:1184	The degradation of the composites in the presence of CA and SF was significantly delayed such that the degradation rate of the implant could satisfy the growth rate of the newly formed bone tissue.
27314146	2	12	theme	matrices	549:556	arg1	modulation					518:527	the synergistic modulation	502:527	the synergistic modulation of the dual organic matrices	502:556	With the synergistic modulation of the dual organic matrices, the HA nanoparticles presented sheet and rod morphologies due to the preferred orientation, which successfully simulated the biomineralization in nature.
27314146	2	13	theme	dual	536:539	arg1	matrices					549:556	the dual organic matrices	532:556	the dual organic matrices	532:556	With the synergistic modulation of the dual organic matrices, the HA nanoparticles presented sheet and rod morphologies due to the preferred orientation, which successfully simulated the biomineralization in nature.
27314146	1	14	theme	biological	384:393	arg1	properties					395:404	optimal physicochemical and biological properties	356:404	optimal physicochemical and biological properties	356:404	By in situ combining the dual cross-linking matrices of the carboxylated agarose (CA) and the silk fibroin (SF) with the hydroxyapatite (HA) crystals, the CA-SF/HA composites with optimal physicochemical and biological properties were obtained, which were designed to meet the clinical needs of load-bearing bone repair.
27314146	5	15	theme	bone	1174:1177	arg1	tissue					1179:1184	the newly formed bone tissue	1157:1184	the newly formed bone tissue	1157:1184	The degradation of the composites in the presence of CA and SF was significantly delayed such that the degradation rate of the implant could satisfy the growth rate of the newly formed bone tissue.
27314146	1	16	theme	carboxylated	236:247	arg1	CA					258:259	CA	258:259	CA	258:259	By in situ combining the dual cross-linking matrices of the carboxylated agarose (CA) and the silk fibroin (SF) with the hydroxyapatite (HA) crystals, the CA-SF/HA composites with optimal physicochemical and biological properties were obtained, which were designed to meet the clinical needs of load-bearing bone repair.
27314146	1	16	theme	carboxylated	236:247	arg1	agarose					249:255	the carboxylated agarose	232:255	the carboxylated agarose (CA)	232:260	By in situ combining the dual cross-linking matrices of the carboxylated agarose (CA) and the silk fibroin (SF) with the hydroxyapatite (HA) crystals, the CA-SF/HA composites with optimal physicochemical and biological properties were obtained, which were designed to meet the clinical needs of load-bearing bone repair.
27314146	5	17	from	composites	1012:1021	arg1	presence					1030:1037	the presence	1026:1037	the presence of CA and SF	1026:1050	The degradation of the composites in the presence of CA and SF was significantly delayed such that the degradation rate of the implant could satisfy the growth rate of the newly formed bone tissue.
27314146	1	18	theme	hydroxyapatite	297:310	arg1	crystals					317:324	the hydroxyapatite (HA) crystals	293:324	the hydroxyapatite (HA) crystals	293:324	By in situ combining the dual cross-linking matrices of the carboxylated agarose (CA) and the silk fibroin (SF) with the hydroxyapatite (HA) crystals, the CA-SF/HA composites with optimal physicochemical and biological properties were obtained, which were designed to meet the clinical needs of load-bearing bone repair.
27314146	2	19	theme	sheet	590:594	arg1	morphologies					604:615	sheet and rod morphologies	590:615	sheet and rod morphologies	590:615	With the synergistic modulation of the dual organic matrices, the HA nanoparticles presented sheet and rod morphologies due to the preferred orientation, which successfully simulated the biomineralization in nature.
27314146	1	20	theme	bone	484:487	arg1	repair					489:494	load-bearing bone repair	471:494	load-bearing bone repair	471:494	By in situ combining the dual cross-linking matrices of the carboxylated agarose (CA) and the silk fibroin (SF) with the hydroxyapatite (HA) crystals, the CA-SF/HA composites with optimal physicochemical and biological properties were obtained, which were designed to meet the clinical needs of load-bearing bone repair.
27314146	1	21	theme	agarose	249:255	arg1	matrices					220:227	the dual cross-linking matrices	197:227	the dual cross-linking matrices of the carboxylated agarose (CA) and the silk fibroin (SF)	197:286	By in situ combining the dual cross-linking matrices of the carboxylated agarose (CA) and the silk fibroin (SF) with the hydroxyapatite (HA) crystals, the CA-SF/HA composites with optimal physicochemical and biological properties were obtained, which were designed to meet the clinical needs of load-bearing bone repair.
27314146	0	22	theme	Hydroxyapatite	88:101	arg1	Crystals					108:115	Oriented Hydroxyapatite (HA) Crystals	79:115	Oriented Hydroxyapatite (HA) Crystals: Biomimetic Organic/Inorganic Composites for Tibia Repair	79:173	Carboxylated Agarose (CA)-Silk Fibroin (SF) Dual Confluent Matrices Containing Oriented Hydroxyapatite (HA) Crystals: Biomimetic Organic/Inorganic Composites for Tibia Repair.
27314146	7	23	theme	tibia	1496:1500	arg1	site					1509:1512	the tibia defect site	1492:1512	the tibia defect site	1492:1512	After 24 days of implantation, newly formed bone was observed at the tibia defect site and around the implant.
27314146	4	24	theme	bone	976:979	arg1	repair					981:986	bone repair	976:986	bone repair	976:986	In the presence of SF, the composites showed optimal mechanical properties that could meet the standard of bone repair.
27314146	1	25	theme	repair	489:494	arg1	clinical					453:460	clinical	453:460	clinical	453:460	By in situ combining the dual cross-linking matrices of the carboxylated agarose (CA) and the silk fibroin (SF) with the hydroxyapatite (HA) crystals, the CA-SF/HA composites with optimal physicochemical and biological properties were obtained, which were designed to meet the clinical needs of load-bearing bone repair.
27314146	0	26	theme	Oriented	79:86	arg1	Crystals					108:115	Oriented Hydroxyapatite (HA) Crystals	79:115	Oriented Hydroxyapatite (HA) Crystals: Biomimetic Organic/Inorganic Composites for Tibia Repair	79:173	Carboxylated Agarose (CA)-Silk Fibroin (SF) Dual Confluent Matrices Containing Oriented Hydroxyapatite (HA) Crystals: Biomimetic Organic/Inorganic Composites for Tibia Repair.
27314146	2	27	theme	HA	563:564	arg1	nanoparticles					566:578	the HA nanoparticles	559:578	the HA nanoparticles	559:578	With the synergistic modulation of the dual organic matrices, the HA nanoparticles presented sheet and rod morphologies due to the preferred orientation, which successfully simulated the biomineralization in nature.
27314146	3	28	theme	NA	760:761	arg1	reactivity					726:735	The chemical reactivity	713:735	The chemical reactivity of the native agarose (NA)	713:762	The chemical reactivity of the native agarose (NA) was significantly enhanced via carboxylation, and the CA exhibited higher thermal stability than the NA.
27314146	1	29	with	composites	340:349	arg1	properties					395:404	optimal physicochemical and biological properties	356:404	optimal physicochemical and biological properties	356:404	By in situ combining the dual cross-linking matrices of the carboxylated agarose (CA) and the silk fibroin (SF) with the hydroxyapatite (HA) crystals, the CA-SF/HA composites with optimal physicochemical and biological properties were obtained, which were designed to meet the clinical needs of load-bearing bone repair.
27314146	3	30	theme	chemical	717:724	arg1	reactivity					726:735	The chemical reactivity	713:735	The chemical reactivity of the native agarose (NA)	713:762	The chemical reactivity of the native agarose (NA) was significantly enhanced via carboxylation, and the CA exhibited higher thermal stability than the NA.
27314146	0	31	theme	Agarose	13:19	arg1	Matrices					59:66	Carboxylated Agarose (CA)-Silk Fibroin (SF) Dual Confluent Matrices	0:66	Carboxylated Agarose (CA)-Silk Fibroin (SF) Dual Confluent Matrices	0:66	Carboxylated Agarose (CA)-Silk Fibroin (SF) Dual Confluent Matrices Containing Oriented Hydroxyapatite (HA) Crystals: Biomimetic Organic/Inorganic Composites for Tibia Repair.
27314146	6	32	theme	in	1191:1192	arg1	tests					1200:1204	The in vitro tests	1187:1204	The in vitro tests	1187:1204	The in vitro tests confirmed that the CA-SF/HA composite scaffolds enabled the MG63 cells to proliferate and differentiate well, and the CA/HA composite presented greater capability of promoting the cell behaviors than the NA/HA composite.
27314146	9	33	contain	had	1683:1685	arg1	composites					1650:1659	the CA-SF/HA composites	1637:1659	the CA-SF/HA composites prepared in this work	1637:1681	In general, the CA-SF/HA composites prepared in this work had the great potential to be applied for repairing large bone defects.
27314146	9	33	contain	had	1683:1685	arg2	potential					1697:1705	the great potential	1687:1705	the great potential to be applied for repairing large bone defects	1687:1752	In general, the CA-SF/HA composites prepared in this work had the great potential to be applied for repairing large bone defects.
27314146	8	34	theme	CA-SF/HA	1604:1611	arg1	composites					1613:1622	the CA-SF/HA composites	1600:1622	the CA-SF/HA composites	1600:1622	Extensive osteogenesis was presented in the rats treated with the CA-SF/HA composites.
27314146	0	35	theme	Carboxylated	0:11	arg1	Matrices					59:66	Carboxylated Agarose (CA)-Silk Fibroin (SF) Dual Confluent Matrices	0:66	Carboxylated Agarose (CA)-Silk Fibroin (SF) Dual Confluent Matrices	0:66	Carboxylated Agarose (CA)-Silk Fibroin (SF) Dual Confluent Matrices Containing Oriented Hydroxyapatite (HA) Crystals: Biomimetic Organic/Inorganic Composites for Tibia Repair.
27314146	9	36	theme	CA-SF/HA	1641:1648	arg1	composites					1650:1659	the CA-SF/HA composites	1637:1659	the CA-SF/HA composites prepared in this work	1637:1681	In general, the CA-SF/HA composites prepared in this work had the great potential to be applied for repairing large bone defects.
27314146	6	37	theme	composite	1234:1242	arg1	scaffolds					1244:1252	the CA-SF/HA composite scaffolds	1221:1252	the CA-SF/HA composite scaffolds	1221:1252	The in vitro tests confirmed that the CA-SF/HA composite scaffolds enabled the MG63 cells to proliferate and differentiate well, and the CA/HA composite presented greater capability of promoting the cell behaviors than the NA/HA composite.
27314146	0	38	theme	CA	22:23	arg1	Matrices					59:66	Carboxylated Agarose (CA)-Silk Fibroin (SF) Dual Confluent Matrices	0:66	Carboxylated Agarose (CA)-Silk Fibroin (SF) Dual Confluent Matrices	0:66	Carboxylated Agarose (CA)-Silk Fibroin (SF) Dual Confluent Matrices Containing Oriented Hydroxyapatite (HA) Crystals: Biomimetic Organic/Inorganic Composites for Tibia Repair.
27314146	5	39	theme	degradation	1092:1102	arg1	rate					1104:1107	the degradation rate	1088:1107	the degradation rate of the implant	1088:1122	The degradation of the composites in the presence of CA and SF was significantly delayed such that the degradation rate of the implant could satisfy the growth rate of the newly formed bone tissue.
27314146	5	40	from	degradation	993:1003	arg1	presence					1030:1037	the presence	1026:1037	the presence of CA and SF	1026:1050	The degradation of the composites in the presence of CA and SF was significantly delayed such that the degradation rate of the implant could satisfy the growth rate of the newly formed bone tissue.
27314146	6	41	theme	CA/HA	1324:1328	arg1	composite					1330:1338	the CA/HA composite	1320:1338	the CA/HA composite	1320:1338	The in vitro tests confirmed that the CA-SF/HA composite scaffolds enabled the MG63 cells to proliferate and differentiate well, and the CA/HA composite presented greater capability of promoting the cell behaviors than the NA/HA composite.
27314146	4	42	dep	SF	888:889	arg1	presence					876:883	presence	876:883	presence	876:883	In the presence of SF, the composites showed optimal mechanical properties that could meet the standard of bone repair.
27314146	4	42	dep	SF	888:889	arg1	the					872:874	the	872:874	the	872:874	In the presence of SF, the composites showed optimal mechanical properties that could meet the standard of bone repair.
27314146	1	43	dep	clinical	453:460	arg1	needs					462:466	needs	462:466	needs	462:466	By in situ combining the dual cross-linking matrices of the carboxylated agarose (CA) and the silk fibroin (SF) with the hydroxyapatite (HA) crystals, the CA-SF/HA composites with optimal physicochemical and biological properties were obtained, which were designed to meet the clinical needs of load-bearing bone repair.
27314146	0	44	theme	-Silk	25:29	arg1	Matrices					59:66	Carboxylated Agarose (CA)-Silk Fibroin (SF) Dual Confluent Matrices	0:66	Carboxylated Agarose (CA)-Silk Fibroin (SF) Dual Confluent Matrices	0:66	Carboxylated Agarose (CA)-Silk Fibroin (SF) Dual Confluent Matrices Containing Oriented Hydroxyapatite (HA) Crystals: Biomimetic Organic/Inorganic Composites for Tibia Repair.
27314146	6	45	theme	CA-SF/HA	1225:1232	arg1	scaffolds					1244:1252	the CA-SF/HA composite scaffolds	1221:1252	the CA-SF/HA composite scaffolds	1221:1252	The in vitro tests confirmed that the CA-SF/HA composite scaffolds enabled the MG63 cells to proliferate and differentiate well, and the CA/HA composite presented greater capability of promoting the cell behaviors than the NA/HA composite.
27314146	7	46	theme	implantation	1444:1455	arg1	days					1436:1439	24 days	1433:1439	24 days of implantation	1433:1455	After 24 days of implantation, newly formed bone was observed at the tibia defect site and around the implant.
27314146	6	47	theme	greater	1350:1356	arg1	capability					1358:1367	greater capability	1350:1367	greater capability of promoting the cell behaviors than the NA/HA composite	1350:1424	The in vitro tests confirmed that the CA-SF/HA composite scaffolds enabled the MG63 cells to proliferate and differentiate well, and the CA/HA composite presented greater capability of promoting the cell behaviors than the NA/HA composite.
27314146	6	48	theme	NA/HA	1410:1414	arg1	composite					1416:1424	the NA/HA composite	1406:1424	the NA/HA composite	1406:1424	The in vitro tests confirmed that the CA-SF/HA composite scaffolds enabled the MG63 cells to proliferate and differentiate well, and the CA/HA composite presented greater capability of promoting the cell behaviors than the NA/HA composite.
27314146	2	49	from	biomineralization	684:700	arg1	nature					705:710	nature	705:710	nature	705:710	With the synergistic modulation of the dual organic matrices, the HA nanoparticles presented sheet and rod morphologies due to the preferred orientation, which successfully simulated the biomineralization in nature.
27314146	4	50	theme	mechanical	922:931	arg1	properties					933:942	optimal mechanical properties	914:942	optimal mechanical properties that could meet the standard of bone repair	914:986	In the presence of SF, the composites showed optimal mechanical properties that could meet the standard of bone repair.
27314146	5	51	theme	implant	1116:1122	arg1	rate					1104:1107	the degradation rate	1088:1107	the degradation rate of the implant	1088:1122	The degradation of the composites in the presence of CA and SF was significantly delayed such that the degradation rate of the implant could satisfy the growth rate of the newly formed bone tissue.
27314146	3	52	theme	native	744:749	arg1	NA					760:761	the native agarose (NA)	740:762	the native agarose (NA)	740:762	The chemical reactivity of the native agarose (NA) was significantly enhanced via carboxylation, and the CA exhibited higher thermal stability than the NA.
27314146	7	53	theme	defect	1502:1507	arg1	site					1509:1512	the tibia defect site	1492:1512	the tibia defect site	1492:1512	After 24 days of implantation, newly formed bone was observed at the tibia defect site and around the implant.
27314146	9	54	theme	bone	1741:1744	arg1	defects					1746:1752	large bone defects	1735:1752	large bone defects	1735:1752	In general, the CA-SF/HA composites prepared in this work had the great potential to be applied for repairing large bone defects.
27314146	1	55	theme	silk	270:273	arg1	SF					284:285	SF	284:285	SF	284:285	By in situ combining the dual cross-linking matrices of the carboxylated agarose (CA) and the silk fibroin (SF) with the hydroxyapatite (HA) crystals, the CA-SF/HA composites with optimal physicochemical and biological properties were obtained, which were designed to meet the clinical needs of load-bearing bone repair.
27314146	1	55	theme	silk	270:273	arg1	fibroin					275:281	the silk fibroin	266:281	the silk fibroin (SF)	266:286	By in situ combining the dual cross-linking matrices of the carboxylated agarose (CA) and the silk fibroin (SF) with the hydroxyapatite (HA) crystals, the CA-SF/HA composites with optimal physicochemical and biological properties were obtained, which were designed to meet the clinical needs of load-bearing bone repair.
27314146	0	56	theme	Biomimetic	118:127	arg1	Composites					147:156	Biomimetic Organic/Inorganic Composites	118:156	Oriented Hydroxyapatite (HA) Crystals: Biomimetic Organic/Inorganic Composites for Tibia Repair	79:173	Carboxylated Agarose (CA)-Silk Fibroin (SF) Dual Confluent Matrices Containing Oriented Hydroxyapatite (HA) Crystals: Biomimetic Organic/Inorganic Composites for Tibia Repair.
27314146	1	57	theme	CA-SF/HA	331:338	arg1	composites					340:349	the CA-SF/HA composites	327:349	the CA-SF/HA composites with optimal physicochemical and biological properties	327:404	By in situ combining the dual cross-linking matrices of the carboxylated agarose (CA) and the silk fibroin (SF) with the hydroxyapatite (HA) crystals, the CA-SF/HA composites with optimal physicochemical and biological properties were obtained, which were designed to meet the clinical needs of load-bearing bone repair.
27314146	9	58	theme	large	1735:1739	arg1	defects					1746:1752	large bone defects	1735:1752	large bone defects	1735:1752	In general, the CA-SF/HA composites prepared in this work had the great potential to be applied for repairing large bone defects.
27314146	0	59	theme	Fibroin	31:37	arg1	Matrices					59:66	Carboxylated Agarose (CA)-Silk Fibroin (SF) Dual Confluent Matrices	0:66	Carboxylated Agarose (CA)-Silk Fibroin (SF) Dual Confluent Matrices	0:66	Carboxylated Agarose (CA)-Silk Fibroin (SF) Dual Confluent Matrices Containing Oriented Hydroxyapatite (HA) Crystals: Biomimetic Organic/Inorganic Composites for Tibia Repair.
27314146	1	60	theme	fibroin	275:281	arg1	matrices					220:227	the dual cross-linking matrices	197:227	the dual cross-linking matrices of the carboxylated agarose (CA) and the silk fibroin (SF)	197:286	By in situ combining the dual cross-linking matrices of the carboxylated agarose (CA) and the silk fibroin (SF) with the hydroxyapatite (HA) crystals, the CA-SF/HA composites with optimal physicochemical and biological properties were obtained, which were designed to meet the clinical needs of load-bearing bone repair.
27314146	5	61	dep	satisfy	1130:1136	arg1	such					1078:1081	such	1078:1081	such	1078:1081	The degradation of the composites in the presence of CA and SF was significantly delayed such that the degradation rate of the implant could satisfy the growth rate of the newly formed bone tissue.
27314146	9	62	theme	great	1691:1695	arg1	potential					1697:1705	the great potential	1687:1705	the great potential to be applied for repairing large bone defects	1687:1752	In general, the CA-SF/HA composites prepared in this work had the great potential to be applied for repairing large bone defects.
27314146	5	63	theme	tissue	1179:1184	arg1	rate					1149:1152	the growth rate	1138:1152	the growth rate of the newly formed bone tissue	1138:1184	The degradation of the composites in the presence of CA and SF was significantly delayed such that the degradation rate of the implant could satisfy the growth rate of the newly formed bone tissue.
27314146	5	64	theme	growth	1142:1147	arg1	rate					1149:1152	the growth rate	1138:1152	the growth rate of the newly formed bone tissue	1138:1184	The degradation of the composites in the presence of CA and SF was significantly delayed such that the degradation rate of the implant could satisfy the growth rate of the newly formed bone tissue.
27314146	2	65	theme	synergistic	506:516	arg1	modulation					518:527	the synergistic modulation	502:527	the synergistic modulation of the dual organic matrices	502:556	With the synergistic modulation of the dual organic matrices, the HA nanoparticles presented sheet and rod morphologies due to the preferred orientation, which successfully simulated the biomineralization in nature.
27314146	3	66	theme	thermal	838:844	arg1	stability					846:854	higher thermal stability	831:854	higher thermal stability	831:854	The chemical reactivity of the native agarose (NA) was significantly enhanced via carboxylation, and the CA exhibited higher thermal stability than the NA.
27314146	2	67	theme	preferred	628:636	arg1	orientation					638:648	the preferred orientation	624:648	the preferred orientation	624:648	With the synergistic modulation of the dual organic matrices, the HA nanoparticles presented sheet and rod morphologies due to the preferred orientation, which successfully simulated the biomineralization in nature.
27314146	8	68	theme	Extensive	1538:1546	arg1	osteogenesis					1548:1559	Extensive osteogenesis	1538:1559	Extensive osteogenesis	1538:1559	Extensive osteogenesis was presented in the rats treated with the CA-SF/HA composites.
27314146	0	69	theme	Organic/Inorganic	129:145	arg1	Composites					147:156	Biomimetic Organic/Inorganic Composites	118:156	Oriented Hydroxyapatite (HA) Crystals: Biomimetic Organic/Inorganic Composites for Tibia Repair	79:173	Carboxylated Agarose (CA)-Silk Fibroin (SF) Dual Confluent Matrices Containing Oriented Hydroxyapatite (HA) Crystals: Biomimetic Organic/Inorganic Composites for Tibia Repair.
27314146	1	70	theme	optimal	356:362	arg1	properties					395:404	optimal physicochemical and biological properties	356:404	optimal physicochemical and biological properties	356:404	By in situ combining the dual cross-linking matrices of the carboxylated agarose (CA) and the silk fibroin (SF) with the hydroxyapatite (HA) crystals, the CA-SF/HA composites with optimal physicochemical and biological properties were obtained, which were designed to meet the clinical needs of load-bearing bone repair.
27314146	6	71	dep	in	1191:1192	arg1	vitro					1194:1198	vitro	1194:1198	vitro	1194:1198	The in vitro tests confirmed that the CA-SF/HA composite scaffolds enabled the MG63 cells to proliferate and differentiate well, and the CA/HA composite presented greater capability of promoting the cell behaviors than the NA/HA composite.
27314146	6	72	theme	MG63	1266:1269	arg1	cells					1271:1275	the MG63 cells	1262:1275	the MG63 cells	1262:1275	The in vitro tests confirmed that the CA-SF/HA composite scaffolds enabled the MG63 cells to proliferate and differentiate well, and the CA/HA composite presented greater capability of promoting the cell behaviors than the NA/HA composite.
27314146	1	73	theme	dual	201:204	arg1	matrices					220:227	the dual cross-linking matrices	197:227	the dual cross-linking matrices of the carboxylated agarose (CA) and the silk fibroin (SF)	197:286	By in situ combining the dual cross-linking matrices of the carboxylated agarose (CA) and the silk fibroin (SF) with the hydroxyapatite (HA) crystals, the CA-SF/HA composites with optimal physicochemical and biological properties were obtained, which were designed to meet the clinical needs of load-bearing bone repair.
27314146	1	74	theme	physicochemical	364:378	arg1	properties					395:404	optimal physicochemical and biological properties	356:404	optimal physicochemical and biological properties	356:404	By in situ combining the dual cross-linking matrices of the carboxylated agarose (CA) and the silk fibroin (SF) with the hydroxyapatite (HA) crystals, the CA-SF/HA composites with optimal physicochemical and biological properties were obtained, which were designed to meet the clinical needs of load-bearing bone repair.
27314146	7	75	located	observed	1480:1487	arg1	site					1509:1512	the tibia defect site	1492:1512	the tibia defect site	1492:1512	After 24 days of implantation, newly formed bone was observed at the tibia defect site and around the implant.
27314146	7	75	located	observed	1480:1487	arg2	bone					1471:1474	newly formed bone	1458:1474	newly formed bone	1458:1474	After 24 days of implantation, newly formed bone was observed at the tibia defect site and around the implant.
27314146	0	76	dep	Crystals	108:115	arg1	Composites					147:156	Biomimetic Organic/Inorganic Composites	118:156	Oriented Hydroxyapatite (HA) Crystals: Biomimetic Organic/Inorganic Composites for Tibia Repair	79:173	Carboxylated Agarose (CA)-Silk Fibroin (SF) Dual Confluent Matrices Containing Oriented Hydroxyapatite (HA) Crystals: Biomimetic Organic/Inorganic Composites for Tibia Repair.
27314146	6	77	theme	cell	1386:1389	arg1	behaviors					1391:1399	the cell behaviors	1382:1399	the cell behaviors than the NA/HA composite	1382:1424	The in vitro tests confirmed that the CA-SF/HA composite scaffolds enabled the MG63 cells to proliferate and differentiate well, and the CA/HA composite presented greater capability of promoting the cell behaviors than the NA/HA composite.
27314146	1	78	theme	cross-linking	206:218	arg1	matrices					220:227	the dual cross-linking matrices	197:227	the dual cross-linking matrices of the carboxylated agarose (CA) and the silk fibroin (SF)	197:286	By in situ combining the dual cross-linking matrices of the carboxylated agarose (CA) and the silk fibroin (SF) with the hydroxyapatite (HA) crystals, the CA-SF/HA composites with optimal physicochemical and biological properties were obtained, which were designed to meet the clinical needs of load-bearing bone repair.
27314146	0	79	theme	SF	40:41	arg1	Matrices					59:66	Carboxylated Agarose (CA)-Silk Fibroin (SF) Dual Confluent Matrices	0:66	Carboxylated Agarose (CA)-Silk Fibroin (SF) Dual Confluent Matrices	0:66	Carboxylated Agarose (CA)-Silk Fibroin (SF) Dual Confluent Matrices Containing Oriented Hydroxyapatite (HA) Crystals: Biomimetic Organic/Inorganic Composites for Tibia Repair.
27314146	5	80	theme	CA	1042:1043	arg1	presence					1030:1037	the presence	1026:1037	the presence of CA and SF	1026:1050	The degradation of the composites in the presence of CA and SF was significantly delayed such that the degradation rate of the implant could satisfy the growth rate of the newly formed bone tissue.
27314146	5	81	from	presence	1030:1037	arg1	degradation					993:1003	The degradation	989:1003	The degradation of the composites in the presence of CA and SF	989:1050	The degradation of the composites in the presence of CA and SF was significantly delayed such that the degradation rate of the implant could satisfy the growth rate of the newly formed bone tissue.
27314146	4	82	theme	repair	981:986	arg1	standard					964:971	standard	964:971	standard	964:971	In the presence of SF, the composites showed optimal mechanical properties that could meet the standard of bone repair.
27314146	3	83	theme	agarose	751:757	arg1	NA					760:761	the native agarose (NA)	740:762	the native agarose (NA)	740:762	The chemical reactivity of the native agarose (NA) was significantly enhanced via carboxylation, and the CA exhibited higher thermal stability than the NA.
27987767	10	0	theme	release	1866:1872	arg1	profiles					1874:1881	The Proguanil drug release profiles	1847:1881	The Proguanil drug release profiles	1847:1881	The Proguanil drug release profiles confirmed for the controlled and sustained release from the EC-MgHPO4 matrix, as the material can release up to 87% of its total loaded drug over a 90min period.
27987767	8	1	theme	temperatures	1652:1663	arg1	range					1638:1642	a range	1636:1642	a range of pHs, temperatures, and electrolyte solutions	1636:1690	We found from the analysis that, with a decrease in the size of hydrated radii of ions, the conductivity of EC-MgHPO4 material also observed to be decreased in the order K+>Na+ and the material proved to be mechanically stable and can be operated over a range of pHs, temperatures, and electrolyte solutions.
27987767	4	2	theme	response	780:787	arg1	index					726:730	a Carr's index	717:730	a Carr's index of 11.11%	717:740	From the results, the material found to exhibit good flowing properties with a Carr's index of 11.11%, Hausner ratio of 1.125, and angle of response of 33°.
27987767	4	2	theme	response	780:787	arg1	angle					771:775	angle	771:775	angle of response of 33°	771:794	From the results, the material found to exhibit good flowing properties with a Carr's index of 11.11%, Hausner ratio of 1.125, and angle of response of 33°.
27987767	4	2	theme	response	780:787	arg1	ratio					751:755	Hausner ratio	743:755	Hausner ratio of 1.125	743:764	From the results, the material found to exhibit good flowing properties with a Carr's index of 11.11%, Hausner ratio of 1.125, and angle of response of 33°.
27987767	7	3	theme	Arrhenius	1282:1290	arg1	method					1292:1297	Arrhenius method	1282:1297	Arrhenius method	1282:1297	In addition, the material's conductivity was studied by taking uni-univalent electrolyte solution (KCl and NaCl) indicated that the conductivity follows the order of KCl>NaCl, while the activation energy obtained from Arrhenius method resembled that the conductivity is strongly influenced by the electrolyte type used.
27987767	10	4	theme	Proguanil	1851:1859	arg1	profiles					1874:1881	The Proguanil drug release profiles	1847:1881	The Proguanil drug release profiles	1847:1881	The Proguanil drug release profiles confirmed for the controlled and sustained release from the EC-MgHPO4 matrix, as the material can release up to 87% of its total loaded drug over a 90min period.
27987767	12	5	theme	biosensor	2374:2382	arg1	applications					2449:2460	biosensor, drug delivery, scaffolding, and bioanalytical (quality control) applications	2374:2460	biosensor, drug delivery, scaffolding, and bioanalytical (quality control) applications	2374:2460	Overall, the results of the study confirm for the safer applicability of the composite towards biosensor, drug delivery, scaffolding, and bioanalytical (quality control) applications.
27987767	3	6	theme	water	529:533	arg1	uptake					535:540	water uptake	529:540	water uptake	529:540	For the composite, the other parameters determined includes the water uptake, porosity, thickness, bulk and tapped densities, angle of repose, Carr's index and Hausner ratio.
27987767	3	6	theme	water	529:533	arg1	angle					591:595	angle	591:595	angle of repose, Carr's index and Hausner ratio	591:637	For the composite, the other parameters determined includes the water uptake, porosity, thickness, bulk and tapped densities, angle of repose, Carr's index and Hausner ratio.
27987767	9	7	theme	drug	1706:1709	arg1	loading					1711:1717	the drug loading	1702:1717	the drug loading	1702:1717	Further, the drug loading and efficiency studies indicated that the material can trap up to 80% of Proguanil (antimalarial drug) applied for its loading.
27987767	8	8	theme	solutions	1682:1690	arg1	range					1638:1642	a range	1636:1642	a range of pHs, temperatures, and electrolyte solutions	1636:1690	We found from the analysis that, with a decrease in the size of hydrated radii of ions, the conductivity of EC-MgHPO4 material also observed to be decreased in the order K+>Na+ and the material proved to be mechanically stable and can be operated over a range of pHs, temperatures, and electrolyte solutions.
27987767	6	9	used	used	1023:1026	arg2	analysis					999:1006	the SEM analysis	991:1006	the SEM analysis	991:1006	The surface porosity due to the EC matrices was confirmed by the SEM analysis, which further used for the loading of drug, Proguanil.
27987767	1	10	theme	hydrogen	189:196	arg1	composite					220:228	ethyl cellulose-magnesium hydrogen phosphate (EC-MgHPO4) composite	163:228	ethyl cellulose-magnesium hydrogen phosphate (EC-MgHPO4) composite that can have potential applications in the sensory, pharmaceutical, and biomedical sectors	163:320	In this, a sol-gel method was applied to prepare ethyl cellulose-magnesium hydrogen phosphate (EC-MgHPO4) composite that can have potential applications in the sensory, pharmaceutical, and biomedical sectors.
27987767	12	11	theme	safer	2329:2333	arg1	applicability					2335:2347	the safer applicability	2325:2347	the safer applicability of the composite towards biosensor, drug delivery, scaffolding, and bioanalytical (quality control) applications	2325:2460	Overall, the results of the study confirm for the safer applicability of the composite towards biosensor, drug delivery, scaffolding, and bioanalytical (quality control) applications.
27987767	7	12	theme	activation	1250:1259	arg1	energy					1261:1266	the activation energy	1246:1266	the activation energy obtained from Arrhenius method	1246:1297	In addition, the material's conductivity was studied by taking uni-univalent electrolyte solution (KCl and NaCl) indicated that the conductivity follows the order of KCl>NaCl, while the activation energy obtained from Arrhenius method resembled that the conductivity is strongly influenced by the electrolyte type used.
27987767	9	13	dep	80	1785:1786	arg1	to					1782:1783	to	1782:1783	to	1782:1783	Further, the drug loading and efficiency studies indicated that the material can trap up to 80% of Proguanil (antimalarial drug) applied for its loading.
27987767	12	14	theme	scaffolding	2400:2410	arg1	applications					2449:2460	biosensor, drug delivery, scaffolding, and bioanalytical (quality control) applications	2374:2460	biosensor, drug delivery, scaffolding, and bioanalytical (quality control) applications	2374:2460	Overall, the results of the study confirm for the safer applicability of the composite towards biosensor, drug delivery, scaffolding, and bioanalytical (quality control) applications.
27987767	11	15	theme	liver	2156:2160	arg1	cultures					2133:2140	two different cell cultures	2114:2140	two different cell cultures of BRL-3A rat liver and H9c2 cardiomyoblasts	2114:2185	Finally, the cell viability and proliferation studies tested against two different cell cultures of BRL-3A rat liver and H9c2 cardiomyoblasts indicated the non-toxic nature and safer applicability of the EC-MgHPO4 (25-500μg/mL, 24h).
27987767	1	16	theme	sol-gel	125:131	arg1	method					133:138	a sol-gel method	123:138	a sol-gel method	123:138	In this, a sol-gel method was applied to prepare ethyl cellulose-magnesium hydrogen phosphate (EC-MgHPO4) composite that can have potential applications in the sensory, pharmaceutical, and biomedical sectors.
27987767	7	17	theme	KCl>NaCl	1230:1237	arg1	order					1221:1225	the order	1217:1225	the order of KCl>NaCl	1217:1237	In addition, the material's conductivity was studied by taking uni-univalent electrolyte solution (KCl and NaCl) indicated that the conductivity follows the order of KCl>NaCl, while the activation energy obtained from Arrhenius method resembled that the conductivity is strongly influenced by the electrolyte type used.
27987767	11	18	dep	EC-MgHPO4	2249:2257	arg1	24h					2273:2275	24h	2273:2275	24h	2273:2275	Finally, the cell viability and proliferation studies tested against two different cell cultures of BRL-3A rat liver and H9c2 cardiomyoblasts indicated the non-toxic nature and safer applicability of the EC-MgHPO4 (25-500μg/mL, 24h).
27987767	11	18	dep	EC-MgHPO4	2249:2257	arg1	25-500μg/mL					2260:2270	25-500μg/mL	2260:2270	25-500μg/mL	2260:2270	Finally, the cell viability and proliferation studies tested against two different cell cultures of BRL-3A rat liver and H9c2 cardiomyoblasts indicated the non-toxic nature and safer applicability of the EC-MgHPO4 (25-500μg/mL, 24h).
27987767	4	19	theme	33°	792:794	arg1	response					780:787	response	780:787	response of 33°	780:794	From the results, the material found to exhibit good flowing properties with a Carr's index of 11.11%, Hausner ratio of 1.125, and angle of response of 33°.
27987767	11	20	theme	cardiomyoblasts	2171:2185	arg1	cultures					2133:2140	two different cell cultures	2114:2140	two different cell cultures of BRL-3A rat liver and H9c2 cardiomyoblasts	2114:2185	Finally, the cell viability and proliferation studies tested against two different cell cultures of BRL-3A rat liver and H9c2 cardiomyoblasts indicated the non-toxic nature and safer applicability of the EC-MgHPO4 (25-500μg/mL, 24h).
27987767	10	21	theme	drug	2019:2022	arg1	drug					2019:2022	its total loaded drug	2002:2022	its total loaded drug	2002:2022	The Proguanil drug release profiles confirmed for the controlled and sustained release from the EC-MgHPO4 matrix, as the material can release up to 87% of its total loaded drug over a 90min period.
27987767	10	21	theme	drug	2019:2022	arg1	%					1997:1997	up to 87%	1989:1997	up to 87% of its total loaded drug	1989:2022	The Proguanil drug release profiles confirmed for the controlled and sustained release from the EC-MgHPO4 matrix, as the material can release up to 87% of its total loaded drug over a 90min period.
27987767	12	22	theme	drug	2385:2388	arg1	delivery					2390:2397	drug delivery	2385:2397	drug delivery	2385:2397	Overall, the results of the study confirm for the safer applicability of the composite towards biosensor, drug delivery, scaffolding, and bioanalytical (quality control) applications.
27987767	10	23	theme	90min	2031:2035	arg1	period					2037:2042	a 90min period	2029:2042	a 90min period	2029:2042	The Proguanil drug release profiles confirmed for the controlled and sustained release from the EC-MgHPO4 matrix, as the material can release up to 87% of its total loaded drug over a 90min period.
27987767	5	24	theme	HRTEM	819:823	arg1	analysis					825:832	HRTEM analysis	819:832	HRTEM analysis	819:832	The EDAX spectrum and HRTEM analysis confirmed for the composite formation and the particles size is investigated to be around 52nm.
27987767	11	25	theme	proliferation	2077:2089	arg1	studies					2091:2097	the cell viability and proliferation studies	2054:2097	the cell viability and proliferation studies tested against two different cell cultures of BRL-3A rat liver and H9c2 cardiomyoblasts	2054:2185	Finally, the cell viability and proliferation studies tested against two different cell cultures of BRL-3A rat liver and H9c2 cardiomyoblasts indicated the non-toxic nature and safer applicability of the EC-MgHPO4 (25-500μg/mL, 24h).
27987767	0	26	theme	cellulose-magnesium	64:82	arg1	composite					103:111	ethyl cellulose-magnesium hydrogen phosphate composite	58:111	ethyl cellulose-magnesium hydrogen phosphate composite	58:111	Sustained drug release and electrochemical performance of ethyl cellulose-magnesium hydrogen phosphate composite.
27987767	12	27	theme	composite	2356:2364	arg1	applicability					2335:2347	the safer applicability	2325:2347	the safer applicability of the composite towards biosensor, drug delivery, scaffolding, and bioanalytical (quality control) applications	2325:2460	Overall, the results of the study confirm for the safer applicability of the composite towards biosensor, drug delivery, scaffolding, and bioanalytical (quality control) applications.
27987767	0	28	theme	phosphate	93:101	arg1	composite					103:111	ethyl cellulose-magnesium hydrogen phosphate composite	58:111	ethyl cellulose-magnesium hydrogen phosphate composite	58:111	Sustained drug release and electrochemical performance of ethyl cellulose-magnesium hydrogen phosphate composite.
27987767	1	29	theme	ethyl	163:167	arg1	composite					220:228	ethyl cellulose-magnesium hydrogen phosphate (EC-MgHPO4) composite	163:228	ethyl cellulose-magnesium hydrogen phosphate (EC-MgHPO4) composite that can have potential applications in the sensory, pharmaceutical, and biomedical sectors	163:320	In this, a sol-gel method was applied to prepare ethyl cellulose-magnesium hydrogen phosphate (EC-MgHPO4) composite that can have potential applications in the sensory, pharmaceutical, and biomedical sectors.
27987767	10	30	dep	87	1995:1996	arg1	to					1992:1993	to	1992:1993	to	1992:1993	The Proguanil drug release profiles confirmed for the controlled and sustained release from the EC-MgHPO4 matrix, as the material can release up to 87% of its total loaded drug over a 90min period.
27987767	11	31	theme	safer	2222:2226	arg1	applicability					2228:2240	safer applicability	2222:2240	safer applicability	2222:2240	Finally, the cell viability and proliferation studies tested against two different cell cultures of BRL-3A rat liver and H9c2 cardiomyoblasts indicated the non-toxic nature and safer applicability of the EC-MgHPO4 (25-500μg/mL, 24h).
27987767	9	32	theme	Proguanil	1792:1800	arg1	Proguanil					1792:1800	Proguanil	1792:1800	Proguanil (antimalarial drug) applied for its loading	1792:1844	Further, the drug loading and efficiency studies indicated that the material can trap up to 80% of Proguanil (antimalarial drug) applied for its loading.
27987767	9	32	theme	Proguanil	1792:1800	arg1	drug					1816:1819	antimalarial drug	1803:1819	antimalarial drug	1803:1819	Further, the drug loading and efficiency studies indicated that the material can trap up to 80% of Proguanil (antimalarial drug) applied for its loading.
27987767	9	32	theme	Proguanil	1792:1800	arg1	%					1787:1787	up to 80%	1779:1787	up to 80% of Proguanil (antimalarial drug) applied for its loading	1779:1844	Further, the drug loading and efficiency studies indicated that the material can trap up to 80% of Proguanil (antimalarial drug) applied for its loading.
27987767	9	33	theme	antimalarial	1803:1814	arg1	Proguanil					1792:1800	Proguanil	1792:1800	Proguanil (antimalarial drug) applied for its loading	1792:1844	Further, the drug loading and efficiency studies indicated that the material can trap up to 80% of Proguanil (antimalarial drug) applied for its loading.
27987767	9	33	theme	antimalarial	1803:1814	arg1	drug					1816:1819	antimalarial drug	1803:1819	antimalarial drug	1803:1819	Further, the drug loading and efficiency studies indicated that the material can trap up to 80% of Proguanil (antimalarial drug) applied for its loading.
27987767	11	34	theme	EC-MgHPO4	2249:2257	arg1	applicability					2228:2240	safer applicability	2222:2240	safer applicability	2222:2240	Finally, the cell viability and proliferation studies tested against two different cell cultures of BRL-3A rat liver and H9c2 cardiomyoblasts indicated the non-toxic nature and safer applicability of the EC-MgHPO4 (25-500μg/mL, 24h).
27987767	11	34	theme	EC-MgHPO4	2249:2257	arg1	nature					2211:2216	non-toxic nature	2201:2216	non-toxic nature	2201:2216	Finally, the cell viability and proliferation studies tested against two different cell cultures of BRL-3A rat liver and H9c2 cardiomyoblasts indicated the non-toxic nature and safer applicability of the EC-MgHPO4 (25-500μg/mL, 24h).
27987767	11	35	theme	rat	2152:2154	arg1	liver					2156:2160	BRL-3A rat liver	2145:2160	BRL-3A rat liver	2145:2160	Finally, the cell viability and proliferation studies tested against two different cell cultures of BRL-3A rat liver and H9c2 cardiomyoblasts indicated the non-toxic nature and safer applicability of the EC-MgHPO4 (25-500μg/mL, 24h).
27987767	2	36	theme	instrumental	394:405	arg1	analysis					407:414	the instrumental analysis	390:414	the instrumental analysis	390:414	The formed composite was thoroughly characterized by making use of the instrumental analysis such as UV-Vis, FT-IR, HRTEM, EDAX, SEM and XRD.
27987767	0	37	theme	Sustained	0:8	arg1	release					15:21	Sustained drug release	0:21	Sustained drug release	0:21	Sustained drug release and electrochemical performance of ethyl cellulose-magnesium hydrogen phosphate composite.
27987767	1	38	theme	pharmaceutical	283:296	arg1	sectors					314:320	the sensory, pharmaceutical, and biomedical sectors	270:320	the sensory, pharmaceutical, and biomedical sectors	270:320	In this, a sol-gel method was applied to prepare ethyl cellulose-magnesium hydrogen phosphate (EC-MgHPO4) composite that can have potential applications in the sensory, pharmaceutical, and biomedical sectors.
27987767	12	39	theme	study	2307:2311	arg1	results					2292:2298	the results	2288:2298	the results of the study	2288:2311	Overall, the results of the study confirm for the safer applicability of the composite towards biosensor, drug delivery, scaffolding, and bioanalytical (quality control) applications.
27987767	4	40	theme	good	688:691	arg1	properties					701:710	good flowing properties	688:710	good flowing properties	688:710	From the results, the material found to exhibit good flowing properties with a Carr's index of 11.11%, Hausner ratio of 1.125, and angle of response of 33°.
27987767	6	41	theme	drug	1047:1050	arg1	loading					1036:1042	the loading	1032:1042	the loading of drug, Proguanil	1032:1061	The surface porosity due to the EC matrices was confirmed by the SEM analysis, which further used for the loading of drug, Proguanil.
27987767	6	42	theme	surface	934:940	arg1	porosity					942:949	The surface porosity	930:949	The surface porosity due to the EC matrices	930:972	The surface porosity due to the EC matrices was confirmed by the SEM analysis, which further used for the loading of drug, Proguanil.
27987767	4	43	theme	%	740:740	arg1	index					726:730	a Carr's index	717:730	a Carr's index of 11.11%	717:740	From the results, the material found to exhibit good flowing properties with a Carr's index of 11.11%, Hausner ratio of 1.125, and angle of response of 33°.
27987767	4	43	theme	%	740:740	arg1	angle					771:775	angle	771:775	angle of response of 33°	771:794	From the results, the material found to exhibit good flowing properties with a Carr's index of 11.11%, Hausner ratio of 1.125, and angle of response of 33°.
27987767	4	43	theme	%	740:740	arg1	ratio					751:755	Hausner ratio	743:755	Hausner ratio of 1.125	743:764	From the results, the material found to exhibit good flowing properties with a Carr's index of 11.11%, Hausner ratio of 1.125, and angle of response of 33°.
27987767	11	44	dep	nature	2211:2216	arg1	the					2197:2199	the	2197:2199	the	2197:2199	Finally, the cell viability and proliferation studies tested against two different cell cultures of BRL-3A rat liver and H9c2 cardiomyoblasts indicated the non-toxic nature and safer applicability of the EC-MgHPO4 (25-500μg/mL, 24h).
27987767	0	45	theme	electrochemical	27:41	arg1	performance					43:53	electrochemical performance	27:53	electrochemical performance	27:53	Sustained drug release and electrochemical performance of ethyl cellulose-magnesium hydrogen phosphate composite.
27987767	3	46	theme	repose	600:605	arg1	uptake					535:540	water uptake	529:540	water uptake	529:540	For the composite, the other parameters determined includes the water uptake, porosity, thickness, bulk and tapped densities, angle of repose, Carr's index and Hausner ratio.
27987767	3	46	theme	repose	600:605	arg1	angle					591:595	angle	591:595	angle of repose, Carr's index and Hausner ratio	591:637	For the composite, the other parameters determined includes the water uptake, porosity, thickness, bulk and tapped densities, angle of repose, Carr's index and Hausner ratio.
27987767	4	47	theme	Hausner	743:749	arg1	ratio					751:755	Hausner ratio	743:755	Hausner ratio of 1.125	743:764	From the results, the material found to exhibit good flowing properties with a Carr's index of 11.11%, Hausner ratio of 1.125, and angle of response of 33°.
27987767	8	48	from	decrease	1424:1431	arg1	size					1440:1443	the size	1436:1443	the size of hydrated radii of ions	1436:1469	We found from the analysis that, with a decrease in the size of hydrated radii of ions, the conductivity of EC-MgHPO4 material also observed to be decreased in the order K+>Na+ and the material proved to be mechanically stable and can be operated over a range of pHs, temperatures, and electrolyte solutions.
27987767	7	49	theme	electrolyte	1141:1151	arg1	solution					1153:1160	uni-univalent electrolyte solution	1127:1160	uni-univalent electrolyte solution (KCl and NaCl) indicated that the conductivity follows the order of KCl>NaCl, while the activation energy obtained from Arrhenius method resembled that the conductivity is strongly influenced by the electrolyte type used	1127:1381	In addition, the material's conductivity was studied by taking uni-univalent electrolyte solution (KCl and NaCl) indicated that the conductivity follows the order of KCl>NaCl, while the activation energy obtained from Arrhenius method resembled that the conductivity is strongly influenced by the electrolyte type used.
27987767	11	50	theme	cell	2058:2061	arg1	viability					2063:2071	cell viability	2058:2071	cell viability	2058:2071	Finally, the cell viability and proliferation studies tested against two different cell cultures of BRL-3A rat liver and H9c2 cardiomyoblasts indicated the non-toxic nature and safer applicability of the EC-MgHPO4 (25-500μg/mL, 24h).
27987767	3	51	theme	Hausner	625:631	arg1	ratio					633:637	Hausner ratio	625:637	Hausner ratio	625:637	For the composite, the other parameters determined includes the water uptake, porosity, thickness, bulk and tapped densities, angle of repose, Carr's index and Hausner ratio.
27987767	3	51	theme	Hausner	625:631	arg1	repose					600:605	repose	600:605	repose	600:605	For the composite, the other parameters determined includes the water uptake, porosity, thickness, bulk and tapped densities, angle of repose, Carr's index and Hausner ratio.
27987767	3	52	theme	other	488:492	arg1	parameters					494:503	the other parameters	484:503	the other parameters determined	484:514	For the composite, the other parameters determined includes the water uptake, porosity, thickness, bulk and tapped densities, angle of repose, Carr's index and Hausner ratio.
27987767	1	53	theme	potential	244:252	arg1	applications					254:265	potential applications	244:265	potential applications	244:265	In this, a sol-gel method was applied to prepare ethyl cellulose-magnesium hydrogen phosphate (EC-MgHPO4) composite that can have potential applications in the sensory, pharmaceutical, and biomedical sectors.
27987767	8	54	theme	material	1502:1509	arg1	conductivity					1476:1487	the conductivity	1472:1487	the conductivity of EC-MgHPO4 material	1472:1509	We found from the analysis that, with a decrease in the size of hydrated radii of ions, the conductivity of EC-MgHPO4 material also observed to be decreased in the order K+>Na+ and the material proved to be mechanically stable and can be operated over a range of pHs, temperatures, and electrolyte solutions.
27987767	10	55	theme	drug	1861:1864	arg1	profiles					1874:1881	The Proguanil drug release profiles	1847:1881	The Proguanil drug release profiles	1847:1881	The Proguanil drug release profiles confirmed for the controlled and sustained release from the EC-MgHPO4 matrix, as the material can release up to 87% of its total loaded drug over a 90min period.
27987767	8	56	theme	radii	1457:1461	arg1	size					1440:1443	the size	1436:1443	the size of hydrated radii of ions	1436:1469	We found from the analysis that, with a decrease in the size of hydrated radii of ions, the conductivity of EC-MgHPO4 material also observed to be decreased in the order K+>Na+ and the material proved to be mechanically stable and can be operated over a range of pHs, temperatures, and electrolyte solutions.
27987767	8	57	theme	electrolyte	1670:1680	arg1	solutions					1682:1690	electrolyte solutions	1670:1690	electrolyte solutions	1670:1690	We found from the analysis that, with a decrease in the size of hydrated radii of ions, the conductivity of EC-MgHPO4 material also observed to be decreased in the order K+>Na+ and the material proved to be mechanically stable and can be operated over a range of pHs, temperatures, and electrolyte solutions.
27987767	1	58	theme	sensory	274:280	arg1	sectors					314:320	the sensory, pharmaceutical, and biomedical sectors	270:320	the sensory, pharmaceutical, and biomedical sectors	270:320	In this, a sol-gel method was applied to prepare ethyl cellulose-magnesium hydrogen phosphate (EC-MgHPO4) composite that can have potential applications in the sensory, pharmaceutical, and biomedical sectors.
27987767	10	59	theme	loaded	2012:2017	arg1	drug					2019:2022	its total loaded drug	2002:2022	its total loaded drug	2002:2022	The Proguanil drug release profiles confirmed for the controlled and sustained release from the EC-MgHPO4 matrix, as the material can release up to 87% of its total loaded drug over a 90min period.
27987767	10	60	theme	controlled	1901:1910	arg1	release					1926:1932	the controlled and sustained release	1897:1932	the controlled and sustained release from the EC-MgHPO4 matrix	1897:1958	The Proguanil drug release profiles confirmed for the controlled and sustained release from the EC-MgHPO4 matrix, as the material can release up to 87% of its total loaded drug over a 90min period.
27987767	8	61	theme	ions	1466:1469	arg1	size					1440:1443	the size	1436:1443	the size of hydrated radii of ions	1436:1469	We found from the analysis that, with a decrease in the size of hydrated radii of ions, the conductivity of EC-MgHPO4 material also observed to be decreased in the order K+>Na+ and the material proved to be mechanically stable and can be operated over a range of pHs, temperatures, and electrolyte solutions.
27987767	12	62	theme	delivery	2390:2397	arg1	applications					2449:2460	biosensor, drug delivery, scaffolding, and bioanalytical (quality control) applications	2374:2460	biosensor, drug delivery, scaffolding, and bioanalytical (quality control) applications	2374:2460	Overall, the results of the study confirm for the safer applicability of the composite towards biosensor, drug delivery, scaffolding, and bioanalytical (quality control) applications.
27987767	1	63	theme	cellulose-magnesium	169:187	arg1	composite					220:228	ethyl cellulose-magnesium hydrogen phosphate (EC-MgHPO4) composite	163:228	ethyl cellulose-magnesium hydrogen phosphate (EC-MgHPO4) composite that can have potential applications in the sensory, pharmaceutical, and biomedical sectors	163:320	In this, a sol-gel method was applied to prepare ethyl cellulose-magnesium hydrogen phosphate (EC-MgHPO4) composite that can have potential applications in the sensory, pharmaceutical, and biomedical sectors.
27987767	9	64	theme	loading	1711:1717	arg1	studies					1734:1740	the drug loading and efficiency studies	1702:1740	the drug loading and efficiency studies	1702:1740	Further, the drug loading and efficiency studies indicated that the material can trap up to 80% of Proguanil (antimalarial drug) applied for its loading.
27987767	2	65	theme	formed	327:332	arg1	composite					334:342	The formed composite	323:342	The formed composite	323:342	The formed composite was thoroughly characterized by making use of the instrumental analysis such as UV-Vis, FT-IR, HRTEM, EDAX, SEM and XRD.
27987767	1	66	theme	phosphate	198:206	arg1	composite					220:228	ethyl cellulose-magnesium hydrogen phosphate (EC-MgHPO4) composite	163:228	ethyl cellulose-magnesium hydrogen phosphate (EC-MgHPO4) composite that can have potential applications in the sensory, pharmaceutical, and biomedical sectors	163:320	In this, a sol-gel method was applied to prepare ethyl cellulose-magnesium hydrogen phosphate (EC-MgHPO4) composite that can have potential applications in the sensory, pharmaceutical, and biomedical sectors.
27987767	4	67	theme	1.125	760:764	arg1	index					726:730	a Carr's index	717:730	a Carr's index of 11.11%	717:740	From the results, the material found to exhibit good flowing properties with a Carr's index of 11.11%, Hausner ratio of 1.125, and angle of response of 33°.
27987767	4	67	theme	1.125	760:764	arg1	angle					771:775	angle	771:775	angle of response of 33°	771:794	From the results, the material found to exhibit good flowing properties with a Carr's index of 11.11%, Hausner ratio of 1.125, and angle of response of 33°.
27987767	4	67	theme	1.125	760:764	arg1	ratio					751:755	Hausner ratio	743:755	Hausner ratio of 1.125	743:764	From the results, the material found to exhibit good flowing properties with a Carr's index of 11.11%, Hausner ratio of 1.125, and angle of response of 33°.
27987767	9	68	theme	efficiency	1723:1732	arg1	studies					1734:1740	the drug loading and efficiency studies	1702:1740	the drug loading and efficiency studies	1702:1740	Further, the drug loading and efficiency studies indicated that the material can trap up to 80% of Proguanil (antimalarial drug) applied for its loading.
27987767	10	69	from	matrix	1953:1958	arg1	release					1926:1932	the controlled and sustained release	1897:1932	the controlled and sustained release from the EC-MgHPO4 matrix	1897:1958	The Proguanil drug release profiles confirmed for the controlled and sustained release from the EC-MgHPO4 matrix, as the material can release up to 87% of its total loaded drug over a 90min period.
27987767	8	70	theme	order	1548:1552	arg1	K+>Na+					1554:1559	the order K+>Na+	1544:1559	the order K+>Na+	1544:1559	We found from the analysis that, with a decrease in the size of hydrated radii of ions, the conductivity of EC-MgHPO4 material also observed to be decreased in the order K+>Na+ and the material proved to be mechanically stable and can be operated over a range of pHs, temperatures, and electrolyte solutions.
27987767	1	71	theme	EC-MgHPO4	209:217	arg1	composite					220:228	ethyl cellulose-magnesium hydrogen phosphate (EC-MgHPO4) composite	163:228	ethyl cellulose-magnesium hydrogen phosphate (EC-MgHPO4) composite that can have potential applications in the sensory, pharmaceutical, and biomedical sectors	163:320	In this, a sol-gel method was applied to prepare ethyl cellulose-magnesium hydrogen phosphate (EC-MgHPO4) composite that can have potential applications in the sensory, pharmaceutical, and biomedical sectors.
27987767	12	72	theme	bioanalytical	2417:2429	arg1	applications					2449:2460	biosensor, drug delivery, scaffolding, and bioanalytical (quality control) applications	2374:2460	biosensor, drug delivery, scaffolding, and bioanalytical (quality control) applications	2374:2460	Overall, the results of the study confirm for the safer applicability of the composite towards biosensor, drug delivery, scaffolding, and bioanalytical (quality control) applications.
27987767	10	73	theme	sustained	1916:1924	arg1	release					1926:1932	the controlled and sustained release	1897:1932	the controlled and sustained release from the EC-MgHPO4 matrix	1897:1958	The Proguanil drug release profiles confirmed for the controlled and sustained release from the EC-MgHPO4 matrix, as the material can release up to 87% of its total loaded drug over a 90min period.
27987767	12	74	theme	quality	2432:2438	arg1	biosensor					2374:2382	biosensor	2374:2382	biosensor	2374:2382	Overall, the results of the study confirm for the safer applicability of the composite towards biosensor, drug delivery, scaffolding, and bioanalytical (quality control) applications.
27987767	12	74	theme	quality	2432:2438	arg1	control					2440:2446	quality control	2432:2446	quality control	2432:2446	Overall, the results of the study confirm for the safer applicability of the composite towards biosensor, drug delivery, scaffolding, and bioanalytical (quality control) applications.
27987767	11	75	theme	H9c2	2166:2169	arg1	cardiomyoblasts					2171:2185	H9c2 cardiomyoblasts	2166:2185	H9c2 cardiomyoblasts	2166:2185	Finally, the cell viability and proliferation studies tested against two different cell cultures of BRL-3A rat liver and H9c2 cardiomyoblasts indicated the non-toxic nature and safer applicability of the EC-MgHPO4 (25-500μg/mL, 24h).
27987767	1	76	contain	have	239:242	arg2	applications					254:265	potential applications	244:265	potential applications	244:265	In this, a sol-gel method was applied to prepare ethyl cellulose-magnesium hydrogen phosphate (EC-MgHPO4) composite that can have potential applications in the sensory, pharmaceutical, and biomedical sectors.
27987767	1	76	contain	have	239:242	arg1	composite					220:228	ethyl cellulose-magnesium hydrogen phosphate (EC-MgHPO4) composite	163:228	ethyl cellulose-magnesium hydrogen phosphate (EC-MgHPO4) composite that can have potential applications in the sensory, pharmaceutical, and biomedical sectors	163:320	In this, a sol-gel method was applied to prepare ethyl cellulose-magnesium hydrogen phosphate (EC-MgHPO4) composite that can have potential applications in the sensory, pharmaceutical, and biomedical sectors.
27987767	5	77	theme	EDAX	801:804	arg1	spectrum					806:813	EDAX spectrum	801:813	EDAX spectrum	801:813	The EDAX spectrum and HRTEM analysis confirmed for the composite formation and the particles size is investigated to be around 52nm.
27987767	10	78	theme	EC-MgHPO4	1943:1951	arg1	matrix					1953:1958	the EC-MgHPO4 matrix	1939:1958	the EC-MgHPO4 matrix	1939:1958	The Proguanil drug release profiles confirmed for the controlled and sustained release from the EC-MgHPO4 matrix, as the material can release up to 87% of its total loaded drug over a 90min period.
27987767	5	79	theme	particles	880:888	arg1	size					890:893	the particles size	876:893	the particles size	876:893	The EDAX spectrum and HRTEM analysis confirmed for the composite formation and the particles size is investigated to be around 52nm.
27987767	11	80	theme	cell	2128:2131	arg1	cultures					2133:2140	two different cell cultures	2114:2140	two different cell cultures of BRL-3A rat liver and H9c2 cardiomyoblasts	2114:2185	Finally, the cell viability and proliferation studies tested against two different cell cultures of BRL-3A rat liver and H9c2 cardiomyoblasts indicated the non-toxic nature and safer applicability of the EC-MgHPO4 (25-500μg/mL, 24h).
27987767	11	81	theme	non-toxic	2201:2209	arg1	nature					2211:2216	non-toxic nature	2201:2216	non-toxic nature	2201:2216	Finally, the cell viability and proliferation studies tested against two different cell cultures of BRL-3A rat liver and H9c2 cardiomyoblasts indicated the non-toxic nature and safer applicability of the EC-MgHPO4 (25-500μg/mL, 24h).
27987767	0	82	theme	hydrogen	84:91	arg1	composite					103:111	ethyl cellulose-magnesium hydrogen phosphate composite	58:111	ethyl cellulose-magnesium hydrogen phosphate composite	58:111	Sustained drug release and electrochemical performance of ethyl cellulose-magnesium hydrogen phosphate composite.
27987767	3	83	theme	tapped	573:578	arg1	densities					580:588	tapped densities	573:588	tapped densities	573:588	For the composite, the other parameters determined includes the water uptake, porosity, thickness, bulk and tapped densities, angle of repose, Carr's index and Hausner ratio.
27987767	0	84	theme	composite	103:111	arg1	release					15:21	Sustained drug release	0:21	Sustained drug release	0:21	Sustained drug release and electrochemical performance of ethyl cellulose-magnesium hydrogen phosphate composite.
27987767	0	84	theme	composite	103:111	arg1	performance					43:53	electrochemical performance	27:53	electrochemical performance	27:53	Sustained drug release and electrochemical performance of ethyl cellulose-magnesium hydrogen phosphate composite.
27987767	11	85	theme	different	2118:2126	arg1	cultures					2133:2140	two different cell cultures	2114:2140	two different cell cultures of BRL-3A rat liver and H9c2 cardiomyoblasts	2114:2185	Finally, the cell viability and proliferation studies tested against two different cell cultures of BRL-3A rat liver and H9c2 cardiomyoblasts indicated the non-toxic nature and safer applicability of the EC-MgHPO4 (25-500μg/mL, 24h).
27987767	5	86	theme	composite	852:860	arg1	formation					862:870	the composite formation	848:870	the composite formation	848:870	The EDAX spectrum and HRTEM analysis confirmed for the composite formation and the particles size is investigated to be around 52nm.
27987767	4	87	theme	flowing	693:699	arg1	properties					701:710	good flowing properties	688:710	good flowing properties	688:710	From the results, the material found to exhibit good flowing properties with a Carr's index of 11.11%, Hausner ratio of 1.125, and angle of response of 33°.
27987767	6	88	theme	EC	962:963	arg1	matrices					965:972	the EC matrices	958:972	the EC matrices	958:972	The surface porosity due to the EC matrices was confirmed by the SEM analysis, which further used for the loading of drug, Proguanil.
27987767	11	89	theme	BRL-3A	2145:2150	arg1	liver					2156:2160	BRL-3A rat liver	2145:2160	BRL-3A rat liver	2145:2160	Finally, the cell viability and proliferation studies tested against two different cell cultures of BRL-3A rat liver and H9c2 cardiomyoblasts indicated the non-toxic nature and safer applicability of the EC-MgHPO4 (25-500μg/mL, 24h).
27987767	5	90	dep	spectrum	806:813	arg1	The					797:799	The	797:799	The	797:799	The EDAX spectrum and HRTEM analysis confirmed for the composite formation and the particles size is investigated to be around 52nm.
27987767	2	91	theme	analysis	407:414	arg1	use					383:385	use	383:385	use of the instrumental analysis such as UV-Vis, FT-IR, HRTEM, EDAX, SEM and XRD	383:462	The formed composite was thoroughly characterized by making use of the instrumental analysis such as UV-Vis, FT-IR, HRTEM, EDAX, SEM and XRD.
27987767	0	92	theme	drug	10:13	arg1	release					15:21	Sustained drug release	0:21	Sustained drug release	0:21	Sustained drug release and electrochemical performance of ethyl cellulose-magnesium hydrogen phosphate composite.
27987767	8	93	theme	pHs	1647:1649	arg1	range					1638:1642	a range	1636:1642	a range of pHs, temperatures, and electrolyte solutions	1636:1690	We found from the analysis that, with a decrease in the size of hydrated radii of ions, the conductivity of EC-MgHPO4 material also observed to be decreased in the order K+>Na+ and the material proved to be mechanically stable and can be operated over a range of pHs, temperatures, and electrolyte solutions.
27987767	1	94	theme	biomedical	303:312	arg1	sectors					314:320	the sensory, pharmaceutical, and biomedical sectors	270:320	the sensory, pharmaceutical, and biomedical sectors	270:320	In this, a sol-gel method was applied to prepare ethyl cellulose-magnesium hydrogen phosphate (EC-MgHPO4) composite that can have potential applications in the sensory, pharmaceutical, and biomedical sectors.
27987767	0	95	theme	ethyl	58:62	arg1	composite					103:111	ethyl cellulose-magnesium hydrogen phosphate composite	58:111	ethyl cellulose-magnesium hydrogen phosphate composite	58:111	Sustained drug release and electrochemical performance of ethyl cellulose-magnesium hydrogen phosphate composite.
27987767	7	96	theme	uni-univalent	1127:1139	arg1	solution					1153:1160	uni-univalent electrolyte solution	1127:1160	uni-univalent electrolyte solution (KCl and NaCl) indicated that the conductivity follows the order of KCl>NaCl, while the activation energy obtained from Arrhenius method resembled that the conductivity is strongly influenced by the electrolyte type used	1127:1381	In addition, the material's conductivity was studied by taking uni-univalent electrolyte solution (KCl and NaCl) indicated that the conductivity follows the order of KCl>NaCl, while the activation energy obtained from Arrhenius method resembled that the conductivity is strongly influenced by the electrolyte type used.
27987767	10	97	theme	total	2006:2010	arg1	drug					2019:2022	its total loaded drug	2002:2022	its total loaded drug	2002:2022	The Proguanil drug release profiles confirmed for the controlled and sustained release from the EC-MgHPO4 matrix, as the material can release up to 87% of its total loaded drug over a 90min period.
27987767	7	98	dep	solution	1153:1160	arg1	KCl					1163:1165	KCl	1163:1165	KCl	1163:1165	In addition, the material's conductivity was studied by taking uni-univalent electrolyte solution (KCl and NaCl) indicated that the conductivity follows the order of KCl>NaCl, while the activation energy obtained from Arrhenius method resembled that the conductivity is strongly influenced by the electrolyte type used.
27987767	7	98	dep	solution	1153:1160	arg1	NaCl					1171:1174	NaCl	1171:1174	NaCl	1171:1174	In addition, the material's conductivity was studied by taking uni-univalent electrolyte solution (KCl and NaCl) indicated that the conductivity follows the order of KCl>NaCl, while the activation energy obtained from Arrhenius method resembled that the conductivity is strongly influenced by the electrolyte type used.
27987767	6	99	theme	SEM	995:997	arg1	analysis					999:1006	the SEM analysis	991:1006	the SEM analysis	991:1006	The surface porosity due to the EC matrices was confirmed by the SEM analysis, which further used for the loading of drug, Proguanil.
27987767	11	100	theme	viability	2063:2071	arg1	studies					2091:2097	the cell viability and proliferation studies	2054:2097	the cell viability and proliferation studies tested against two different cell cultures of BRL-3A rat liver and H9c2 cardiomyoblasts	2054:2185	Finally, the cell viability and proliferation studies tested against two different cell cultures of BRL-3A rat liver and H9c2 cardiomyoblasts indicated the non-toxic nature and safer applicability of the EC-MgHPO4 (25-500μg/mL, 24h).
27987767	8	101	theme	EC-MgHPO4	1492:1500	arg1	material					1502:1509	EC-MgHPO4 material	1492:1509	EC-MgHPO4 material	1492:1509	We found from the analysis that, with a decrease in the size of hydrated radii of ions, the conductivity of EC-MgHPO4 material also observed to be decreased in the order K+>Na+ and the material proved to be mechanically stable and can be operated over a range of pHs, temperatures, and electrolyte solutions.
27987767	7	102	theme	electrolyte	1361:1371	arg1	type					1373:1376	the electrolyte type	1357:1376	the electrolyte type used	1357:1381	In addition, the material's conductivity was studied by taking uni-univalent electrolyte solution (KCl and NaCl) indicated that the conductivity follows the order of KCl>NaCl, while the activation energy obtained from Arrhenius method resembled that the conductivity is strongly influenced by the electrolyte type used.
27987767	8	103	theme	hydrated	1448:1455	arg1	radii					1457:1461	hydrated radii	1448:1461	hydrated radii	1448:1461	We found from the analysis that, with a decrease in the size of hydrated radii of ions, the conductivity of EC-MgHPO4 material also observed to be decreased in the order K+>Na+ and the material proved to be mechanically stable and can be operated over a range of pHs, temperatures, and electrolyte solutions.
24664323	0	0	theme	controlled	105:114	arg1	release					116:122	controlled release	105:122	controlled release	105:122	New type of chitosan/2-hydroxypropyl-β-cyclodextrin composite membrane for gallic acid encapsulation and controlled release.
24664323	5	1	theme	tissue	1045:1050	arg1	regeneration					1052:1063	the surrounding tissue regeneration	1029:1063	the surrounding tissue regeneration	1029:1063	The information obtained in this study will facilitate the design and preparation of composite membrane based on chitosan and could open a wide range of applications, particularly its use as an antioxidant in food, food packaging, biomedical (biodegradable soft porous scaffolds for enhance the surrounding tissue regeneration), pharmaceutical and cosmetics industries.
24664323	5	2	theme	soft	995:998	arg1	scaffolds					1007:1015	biodegradable soft porous scaffolds	981:1015	biodegradable soft porous scaffolds for enhance the surrounding tissue regeneration	981:1063	The information obtained in this study will facilitate the design and preparation of composite membrane based on chitosan and could open a wide range of applications, particularly its use as an antioxidant in food, food packaging, biomedical (biodegradable soft porous scaffolds for enhance the surrounding tissue regeneration), pharmaceutical and cosmetics industries.
24664323	5	3	theme	composite	823:831	arg1	membrane					833:840	composite membrane	823:840	composite membrane based on chitosan	823:858	The information obtained in this study will facilitate the design and preparation of composite membrane based on chitosan and could open a wide range of applications, particularly its use as an antioxidant in food, food packaging, biomedical (biodegradable soft porous scaffolds for enhance the surrounding tissue regeneration), pharmaceutical and cosmetics industries.
24664323	2	4	theme	infrared	340:347	arg1	FT-IR					363:367	FT-IR	363:367	FT-IR	363:367	The morphology of the composite membrane was investigated by infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM), whereas swelling gallic acid and release properties were investigated by UV-visible spectroscopy.
24664323	2	4	theme	infrared	340:347	arg1	spectroscopy					349:360	infrared spectroscopy	340:360	infrared spectroscopy (FT-IR)	340:368	The morphology of the composite membrane was investigated by infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM), whereas swelling gallic acid and release properties were investigated by UV-visible spectroscopy.
24664323	5	5	theme	surrounding	1033:1043	arg1	regeneration					1052:1063	the surrounding tissue regeneration	1029:1063	the surrounding tissue regeneration	1029:1063	The information obtained in this study will facilitate the design and preparation of composite membrane based on chitosan and could open a wide range of applications, particularly its use as an antioxidant in food, food packaging, biomedical (biodegradable soft porous scaffolds for enhance the surrounding tissue regeneration), pharmaceutical and cosmetics industries.
24664323	4	6	theme	antioxidant	678:688	arg1	capacities					690:699	improved antioxidant capacities	669:699	improved antioxidant capacities	669:699	The composite membrane based on chitosan/2-hydroxypropyl-β-cyclodextrin with gallic acid included showed improved antioxidant capacities compared to plain chitosan membrane.
24664323	5	7	theme	membrane	833:840	arg1	preparation					808:818	preparation	808:818	preparation	808:818	The information obtained in this study will facilitate the design and preparation of composite membrane based on chitosan and could open a wide range of applications, particularly its use as an antioxidant in food, food packaging, biomedical (biodegradable soft porous scaffolds for enhance the surrounding tissue regeneration), pharmaceutical and cosmetics industries.
24664323	5	7	theme	membrane	833:840	arg1	design					797:802	the design	793:802	the design	793:802	The information obtained in this study will facilitate the design and preparation of composite membrane based on chitosan and could open a wide range of applications, particularly its use as an antioxidant in food, food packaging, biomedical (biodegradable soft porous scaffolds for enhance the surrounding tissue regeneration), pharmaceutical and cosmetics industries.
24664323	5	8	dep	scaffolds	1007:1015	arg1	enhance					1021:1027	enhance	1021:1027	enhance the surrounding tissue regeneration	1021:1063	The information obtained in this study will facilitate the design and preparation of composite membrane based on chitosan and could open a wide range of applications, particularly its use as an antioxidant in food, food packaging, biomedical (biodegradable soft porous scaffolds for enhance the surrounding tissue regeneration), pharmaceutical and cosmetics industries.
24664323	3	9	theme	pH	534:535	arg1	changes					537:543	pH changes	534:543	pH changes	534:543	The release behavior with pH changes was also explored.
24664323	4	10	theme	chitosan	719:726	arg1	membrane					728:735	plain chitosan membrane	713:735	plain chitosan membrane	713:735	The composite membrane based on chitosan/2-hydroxypropyl-β-cyclodextrin with gallic acid included showed improved antioxidant capacities compared to plain chitosan membrane.
24664323	5	11	theme	porous	1000:1005	arg1	scaffolds					1007:1015	biodegradable soft porous scaffolds	981:1015	biodegradable soft porous scaffolds for enhance the surrounding tissue regeneration	981:1063	The information obtained in this study will facilitate the design and preparation of composite membrane based on chitosan and could open a wide range of applications, particularly its use as an antioxidant in food, food packaging, biomedical (biodegradable soft porous scaffolds for enhance the surrounding tissue regeneration), pharmaceutical and cosmetics industries.
24664323	2	12	theme	acid	434:437	arg1	properties					451:460	swelling gallic acid and release properties	418:460	swelling gallic acid and release properties	418:460	The morphology of the composite membrane was investigated by infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM), whereas swelling gallic acid and release properties were investigated by UV-visible spectroscopy.
24664323	4	13	theme	improved	669:676	arg1	capacities					690:699	improved antioxidant capacities	669:699	improved antioxidant capacities	669:699	The composite membrane based on chitosan/2-hydroxypropyl-β-cyclodextrin with gallic acid included showed improved antioxidant capacities compared to plain chitosan membrane.
24664323	2	14	theme	UV-visible	483:492	arg1	spectroscopy					494:505	UV-visible spectroscopy	483:505	UV-visible spectroscopy	483:505	The morphology of the composite membrane was investigated by infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM), whereas swelling gallic acid and release properties were investigated by UV-visible spectroscopy.
24664323	2	15	theme	gallic	427:432	arg1	acid					434:437	swelling gallic acid	418:437	swelling gallic acid	418:437	The morphology of the composite membrane was investigated by infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM), whereas swelling gallic acid and release properties were investigated by UV-visible spectroscopy.
24664323	5	16	theme	applications	891:902	arg1	range					882:886	a wide range	875:886	a wide range of applications, particularly its use as an antioxidant in food, food packaging, biomedical (biodegradable soft porous scaffolds for enhance the surrounding tissue regeneration), pharmaceutical and cosmetics industries	875:1105	The information obtained in this study will facilitate the design and preparation of composite membrane based on chitosan and could open a wide range of applications, particularly its use as an antioxidant in food, food packaging, biomedical (biodegradable soft porous scaffolds for enhance the surrounding tissue regeneration), pharmaceutical and cosmetics industries.
24664323	5	17	dep	biomedical	969:978	arg1	scaffolds					1007:1015	biodegradable soft porous scaffolds	981:1015	biodegradable soft porous scaffolds for enhance the surrounding tissue regeneration	981:1063	The information obtained in this study will facilitate the design and preparation of composite membrane based on chitosan and could open a wide range of applications, particularly its use as an antioxidant in food, food packaging, biomedical (biodegradable soft porous scaffolds for enhance the surrounding tissue regeneration), pharmaceutical and cosmetics industries.
24664323	2	18	theme	swelling	418:425	arg1	acid					434:437	swelling gallic acid	418:437	swelling gallic acid	418:437	The morphology of the composite membrane was investigated by infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM), whereas swelling gallic acid and release properties were investigated by UV-visible spectroscopy.
24664323	0	19	theme	New	0:2	arg1	type					4:7	New type	0:7	New type of chitosan/2-hydroxypropyl-β-cyclodextrin composite membrane for gallic acid encapsulation and controlled release.	0:123	New type of chitosan/2-hydroxypropyl-β-cyclodextrin composite membrane for gallic acid encapsulation and controlled release.
24664323	5	20	theme	food	953:956	arg1	packaging					958:966	food packaging	953:966	food packaging	953:966	The information obtained in this study will facilitate the design and preparation of composite membrane based on chitosan and could open a wide range of applications, particularly its use as an antioxidant in food, food packaging, biomedical (biodegradable soft porous scaffolds for enhance the surrounding tissue regeneration), pharmaceutical and cosmetics industries.
24664323	0	21	theme	chitosan/2-hydroxypropyl-β-cyclodextrin	12:50	arg1	membrane					62:69	chitosan/2-hydroxypropyl-β-cyclodextrin composite membrane	12:69	chitosan/2-hydroxypropyl-β-cyclodextrin composite membrane	12:69	New type of chitosan/2-hydroxypropyl-β-cyclodextrin composite membrane for gallic acid encapsulation and controlled release.
24664323	1	22	theme	controlled	244:253	arg1	release					255:261	controlled release	244:261	controlled release	244:261	A new type of chitosan/2-hydroxypropyl-β-cyclodextrin composite membrane have been developed for the encapsulation and controlled release of gallic acid.
24664323	1	23	theme	new	127:129	arg1	type					131:134	A new type	125:134	A new type of chitosan/2-hydroxypropyl-β-cyclodextrin composite membrane	125:196	A new type of chitosan/2-hydroxypropyl-β-cyclodextrin composite membrane have been developed for the encapsulation and controlled release of gallic acid.
24664323	2	24	dep	investigated	324:335	arg1	whereas					410:416	whereas	410:416	whereas	410:416	The morphology of the composite membrane was investigated by infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM), whereas swelling gallic acid and release properties were investigated by UV-visible spectroscopy.
24664323	0	25	theme	membrane	62:69	arg1	type					4:7	New type	0:7	New type of chitosan/2-hydroxypropyl-β-cyclodextrin composite membrane for gallic acid encapsulation and controlled release.	0:123	New type of chitosan/2-hydroxypropyl-β-cyclodextrin composite membrane for gallic acid encapsulation and controlled release.
24664323	4	26	theme	gallic	641:646	arg1	acid					648:651	gallic acid	641:651	gallic acid	641:651	The composite membrane based on chitosan/2-hydroxypropyl-β-cyclodextrin with gallic acid included showed improved antioxidant capacities compared to plain chitosan membrane.
24664323	1	27	dep	encapsulation	226:238	arg1	the					222:224	the	222:224	the	222:224	A new type of chitosan/2-hydroxypropyl-β-cyclodextrin composite membrane have been developed for the encapsulation and controlled release of gallic acid.
24664323	2	28	theme	release	443:449	arg1	properties					451:460	swelling gallic acid and release properties	418:460	swelling gallic acid and release properties	418:460	The morphology of the composite membrane was investigated by infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM), whereas swelling gallic acid and release properties were investigated by UV-visible spectroscopy.
24664323	0	29	theme	composite	52:60	arg1	membrane					62:69	chitosan/2-hydroxypropyl-β-cyclodextrin composite membrane	12:69	chitosan/2-hydroxypropyl-β-cyclodextrin composite membrane	12:69	New type of chitosan/2-hydroxypropyl-β-cyclodextrin composite membrane for gallic acid encapsulation and controlled release.
24664323	3	30	with	behavior	520:527	arg1	changes					537:543	pH changes	534:543	pH changes	534:543	The release behavior with pH changes was also explored.
24664323	1	31	theme	gallic	266:271	arg1	acid					273:276	gallic acid	266:276	gallic acid	266:276	A new type of chitosan/2-hydroxypropyl-β-cyclodextrin composite membrane have been developed for the encapsulation and controlled release of gallic acid.
24664323	0	32	theme	gallic	75:80	arg1	encapsulation					87:99	gallic acid encapsulation	75:99	gallic acid encapsulation	75:99	New type of chitosan/2-hydroxypropyl-β-cyclodextrin composite membrane for gallic acid encapsulation and controlled release.
24664323	4	33	theme	composite	568:576	arg1	membrane					578:585	The composite membrane	564:585	The composite membrane based on chitosan/2-hydroxypropyl-β-cyclodextrin with gallic acid	564:651	The composite membrane based on chitosan/2-hydroxypropyl-β-cyclodextrin with gallic acid included showed improved antioxidant capacities compared to plain chitosan membrane.
24664323	4	34	dep	included	653:660	arg1	showed					662:667	showed	662:667	included showed improved antioxidant capacities compared to plain chitosan membrane	653:735	The composite membrane based on chitosan/2-hydroxypropyl-β-cyclodextrin with gallic acid included showed improved antioxidant capacities compared to plain chitosan membrane.
24664323	5	35	theme	biodegradable	981:993	arg1	scaffolds					1007:1015	biodegradable soft porous scaffolds	981:1015	biodegradable soft porous scaffolds for enhance the surrounding tissue regeneration	981:1063	The information obtained in this study will facilitate the design and preparation of composite membrane based on chitosan and could open a wide range of applications, particularly its use as an antioxidant in food, food packaging, biomedical (biodegradable soft porous scaffolds for enhance the surrounding tissue regeneration), pharmaceutical and cosmetics industries.
24664323	5	36	dep	food	947:950	arg1	industries					1096:1105	industries	1096:1105	industries	1096:1105	The information obtained in this study will facilitate the design and preparation of composite membrane based on chitosan and could open a wide range of applications, particularly its use as an antioxidant in food, food packaging, biomedical (biodegradable soft porous scaffolds for enhance the surrounding tissue regeneration), pharmaceutical and cosmetics industries.
24664323	5	37	from	use	922:924	arg1	biomedical					969:978	biomedical	969:978	biomedical	969:978	The information obtained in this study will facilitate the design and preparation of composite membrane based on chitosan and could open a wide range of applications, particularly its use as an antioxidant in food, food packaging, biomedical (biodegradable soft porous scaffolds for enhance the surrounding tissue regeneration), pharmaceutical and cosmetics industries.
24664323	5	37	from	use	922:924	arg1	food					947:950	food	947:950	food	947:950	The information obtained in this study will facilitate the design and preparation of composite membrane based on chitosan and could open a wide range of applications, particularly its use as an antioxidant in food, food packaging, biomedical (biodegradable soft porous scaffolds for enhance the surrounding tissue regeneration), pharmaceutical and cosmetics industries.
24664323	5	37	from	use	922:924	arg1	packaging					958:966	food packaging	953:966	food packaging	953:966	The information obtained in this study will facilitate the design and preparation of composite membrane based on chitosan and could open a wide range of applications, particularly its use as an antioxidant in food, food packaging, biomedical (biodegradable soft porous scaffolds for enhance the surrounding tissue regeneration), pharmaceutical and cosmetics industries.
24664323	5	37	from	use	922:924	arg1	pharmaceutical					1067:1080	pharmaceutical	1067:1080	pharmaceutical	1067:1080	The information obtained in this study will facilitate the design and preparation of composite membrane based on chitosan and could open a wide range of applications, particularly its use as an antioxidant in food, food packaging, biomedical (biodegradable soft porous scaffolds for enhance the surrounding tissue regeneration), pharmaceutical and cosmetics industries.
24664323	5	37	from	use	922:924	arg1	cosmetics					1086:1094	cosmetics	1086:1094	cosmetics	1086:1094	The information obtained in this study will facilitate the design and preparation of composite membrane based on chitosan and could open a wide range of applications, particularly its use as an antioxidant in food, food packaging, biomedical (biodegradable soft porous scaffolds for enhance the surrounding tissue regeneration), pharmaceutical and cosmetics industries.
24664323	2	38	theme	electron	383:390	arg1	SEM					404:406	SEM	404:406	SEM	404:406	The morphology of the composite membrane was investigated by infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM), whereas swelling gallic acid and release properties were investigated by UV-visible spectroscopy.
24664323	2	38	theme	electron	383:390	arg1	microscopy					392:401	scanning electron microscopy	374:401	scanning electron microscopy (SEM)	374:407	The morphology of the composite membrane was investigated by infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM), whereas swelling gallic acid and release properties were investigated by UV-visible spectroscopy.
24664323	2	39	theme	membrane	311:318	arg1	morphology					283:292	The morphology	279:292	The morphology of the composite membrane	279:318	The morphology of the composite membrane was investigated by infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM), whereas swelling gallic acid and release properties were investigated by UV-visible spectroscopy.
24664323	1	40	theme	chitosan/2-hydroxypropyl-β-cyclodextrin	139:177	arg1	membrane					189:196	chitosan/2-hydroxypropyl-β-cyclodextrin composite membrane	139:196	chitosan/2-hydroxypropyl-β-cyclodextrin composite membrane	139:196	A new type of chitosan/2-hydroxypropyl-β-cyclodextrin composite membrane have been developed for the encapsulation and controlled release of gallic acid.
24664323	1	41	theme	acid	273:276	arg1	encapsulation					226:238	encapsulation	226:238	encapsulation	226:238	A new type of chitosan/2-hydroxypropyl-β-cyclodextrin composite membrane have been developed for the encapsulation and controlled release of gallic acid.
24664323	1	41	theme	acid	273:276	arg1	release					255:261	controlled release	244:261	controlled release	244:261	A new type of chitosan/2-hydroxypropyl-β-cyclodextrin composite membrane have been developed for the encapsulation and controlled release of gallic acid.
24664323	4	42	with	chitosan/2-hydroxypropyl-β-cyclodextrin	596:634	arg1	acid					648:651	gallic acid	641:651	gallic acid	641:651	The composite membrane based on chitosan/2-hydroxypropyl-β-cyclodextrin with gallic acid included showed improved antioxidant capacities compared to plain chitosan membrane.
24664323	2	43	theme	composite	301:309	arg1	membrane					311:318	the composite membrane	297:318	the composite membrane	297:318	The morphology of the composite membrane was investigated by infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM), whereas swelling gallic acid and release properties were investigated by UV-visible spectroscopy.
24664323	1	44	theme	composite	179:187	arg1	membrane					189:196	chitosan/2-hydroxypropyl-β-cyclodextrin composite membrane	139:196	chitosan/2-hydroxypropyl-β-cyclodextrin composite membrane	139:196	A new type of chitosan/2-hydroxypropyl-β-cyclodextrin composite membrane have been developed for the encapsulation and controlled release of gallic acid.
24664323	2	45	theme	scanning	374:381	arg1	SEM					404:406	SEM	404:406	SEM	404:406	The morphology of the composite membrane was investigated by infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM), whereas swelling gallic acid and release properties were investigated by UV-visible spectroscopy.
24664323	2	45	theme	scanning	374:381	arg1	microscopy					392:401	scanning electron microscopy	374:401	scanning electron microscopy (SEM)	374:407	The morphology of the composite membrane was investigated by infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM), whereas swelling gallic acid and release properties were investigated by UV-visible spectroscopy.
24664323	3	46	theme	release	512:518	arg1	behavior					520:527	The release behavior	508:527	The release behavior with pH changes	508:543	The release behavior with pH changes was also explored.
24664323	1	47	theme	membrane	189:196	arg1	type					131:134	A new type	125:134	A new type of chitosan/2-hydroxypropyl-β-cyclodextrin composite membrane	125:196	A new type of chitosan/2-hydroxypropyl-β-cyclodextrin composite membrane have been developed for the encapsulation and controlled release of gallic acid.
24664323	4	48	theme	plain	713:717	arg1	membrane					728:735	plain chitosan membrane	713:735	plain chitosan membrane	713:735	The composite membrane based on chitosan/2-hydroxypropyl-β-cyclodextrin with gallic acid included showed improved antioxidant capacities compared to plain chitosan membrane.
24664323	0	49	theme	acid	82:85	arg1	encapsulation					87:99	gallic acid encapsulation	75:99	gallic acid encapsulation	75:99	New type of chitosan/2-hydroxypropyl-β-cyclodextrin composite membrane for gallic acid encapsulation and controlled release.
24664323	5	50	theme	wide	877:880	arg1	range					882:886	a wide range	875:886	a wide range of applications, particularly its use as an antioxidant in food, food packaging, biomedical (biodegradable soft porous scaffolds for enhance the surrounding tissue regeneration), pharmaceutical and cosmetics industries	875:1105	The information obtained in this study will facilitate the design and preparation of composite membrane based on chitosan and could open a wide range of applications, particularly its use as an antioxidant in food, food packaging, biomedical (biodegradable soft porous scaffolds for enhance the surrounding tissue regeneration), pharmaceutical and cosmetics industries.
28065751	3	0	theme	cross-linking	690:702	arg1	extents					704:710	different cross-linking extents	680:710	different cross-linking extents	680:710	Thermal stability, anti-biodegradability, mechanical property and surface hydrophobicity of the composites with different cross-linking extents and drug loading were systematically investigated.
28065751	6	1	contain	had	1080:1082	arg1	system					1073:1078	The developed drug delivery system	1045:1078	The developed drug delivery system	1045:1078	The developed drug delivery system had profound significance in improving pesticide effect and bioavailability of drugs.
28065751	6	1	contain	had	1080:1082	arg2	significance					1093:1104	profound significance	1084:1104	profound significance	1084:1104	The developed drug delivery system had profound significance in improving pesticide effect and bioavailability of drugs.
28065751	0	2	theme	ester	83:87	arg1	network					39:45	Honeycomb structural composite polymer network	0:45	Honeycomb structural composite polymer network of gelatin and functional cellulose ester for controlled release of omeprazole.	0:125	Honeycomb structural composite polymer network of gelatin and functional cellulose ester for controlled release of omeprazole.
28065751	3	3	theme	composites	664:673	arg1	property					621:628	mechanical property	610:628	mechanical property	610:628	Thermal stability, anti-biodegradability, mechanical property and surface hydrophobicity of the composites with different cross-linking extents and drug loading were systematically investigated.
28065751	3	3	theme	composites	664:673	arg1	stability					576:584	Thermal stability	568:584	Thermal stability	568:584	Thermal stability, anti-biodegradability, mechanical property and surface hydrophobicity of the composites with different cross-linking extents and drug loading were systematically investigated.
28065751	3	3	theme	composites	664:673	arg1	anti-biodegradability					587:607	anti-biodegradability	587:607	anti-biodegradability	587:607	Thermal stability, anti-biodegradability, mechanical property and surface hydrophobicity of the composites with different cross-linking extents and drug loading were systematically investigated.
28065751	3	3	theme	composites	664:673	arg1	hydrophobicity					642:655	surface hydrophobicity	634:655	surface hydrophobicity	634:655	Thermal stability, anti-biodegradability, mechanical property and surface hydrophobicity of the composites with different cross-linking extents and drug loading were systematically investigated.
28065751	3	4	theme	surface	634:640	arg1	hydrophobicity					642:655	surface hydrophobicity	634:655	surface hydrophobicity	634:655	Thermal stability, anti-biodegradability, mechanical property and surface hydrophobicity of the composites with different cross-linking extents and drug loading were systematically investigated.
28065751	6	5	theme	pesticide	1119:1127	arg1	effect					1129:1134	pesticide effect	1119:1134	pesticide effect	1119:1134	The developed drug delivery system had profound significance in improving pesticide effect and bioavailability of drugs.
28065751	1	6	theme	active	262:267	arg1	groups					275:280	gelatin and active ester groups	250:280	gelatin and active ester groups in MCN	250:287	The functionalized cellulose ester MCN was firstly synthesized and used to cross-link gelatin by amidation between -NH2 in gelatin and active ester groups in MCN to form a composite polymer network Gel-MCN, which was confirmed by Van Slyke method, FTIR, XRD and TGA-DTG spectra.
28065751	2	7	theme	hydrogen	505:512	arg1	bonds					514:518	hydrogen bonds	505:518	hydrogen bonds	505:518	The model drug omeprazole was loaded in Gel-MCN composites mainly by electrostatic interaction and hydrogen bonds, which were certified by FTIR, XRD and TGA-DSC.
28065751	4	8	theme	honeycomb	791:799	arg1	cells					812:816	the honeycomb structural cells	787:816	the honeycomb structural cells of cross-linked gelatin networks	787:849	SEM images demonstrated the honeycomb structural cells of cross-linked gelatin networks and this ensured drug entrapment.
28065751	1	9	theme	ester	269:273	arg1	groups					275:280	gelatin and active ester groups	250:280	gelatin and active ester groups in MCN	250:287	The functionalized cellulose ester MCN was firstly synthesized and used to cross-link gelatin by amidation between -NH2 in gelatin and active ester groups in MCN to form a composite polymer network Gel-MCN, which was confirmed by Van Slyke method, FTIR, XRD and TGA-DTG spectra.
28065751	0	10	theme	controlled	93:102	arg1	release					104:110	controlled release	93:110	controlled release of omeprazole	93:124	Honeycomb structural composite polymer network of gelatin and functional cellulose ester for controlled release of omeprazole.
28065751	3	11	theme	drug	716:719	arg1	loading					721:727	drug loading	716:727	drug loading	716:727	Thermal stability, anti-biodegradability, mechanical property and surface hydrophobicity of the composites with different cross-linking extents and drug loading were systematically investigated.
28065751	4	12	theme	SEM	763:765	arg1	images					767:772	SEM images	763:772	SEM images	763:772	SEM images demonstrated the honeycomb structural cells of cross-linked gelatin networks and this ensured drug entrapment.
28065751	0	13	theme	omeprazole	115:124	arg1	release					104:110	controlled release	93:110	controlled release of omeprazole	93:124	Honeycomb structural composite polymer network of gelatin and functional cellulose ester for controlled release of omeprazole.
28065751	5	14	theme	release	985:991	arg1	rate					993:996	the release rate	981:996	the release rate decreased with cross-linking degree	981:1032	The drug release mechanism was dominated by a combined effect of diffusion and degradation, and the release rate decreased with cross-linking degree increased.
28065751	3	15	with	hydrophobicity	642:655	arg1	extents					704:710	different cross-linking extents	680:710	different cross-linking extents	680:710	Thermal stability, anti-biodegradability, mechanical property and surface hydrophobicity of the composites with different cross-linking extents and drug loading were systematically investigated.
28065751	3	15	with	hydrophobicity	642:655	arg1	loading					721:727	drug loading	716:727	drug loading	716:727	Thermal stability, anti-biodegradability, mechanical property and surface hydrophobicity of the composites with different cross-linking extents and drug loading were systematically investigated.
28065751	2	16	theme	electrostatic	475:487	arg1	interaction					489:499	electrostatic interaction	475:499	electrostatic interaction	475:499	The model drug omeprazole was loaded in Gel-MCN composites mainly by electrostatic interaction and hydrogen bonds, which were certified by FTIR, XRD and TGA-DSC.
28065751	2	17	theme	Gel-MCN	446:452	arg1	composites					454:463	Gel-MCN composites	446:463	Gel-MCN composites	446:463	The model drug omeprazole was loaded in Gel-MCN composites mainly by electrostatic interaction and hydrogen bonds, which were certified by FTIR, XRD and TGA-DSC.
28065751	2	18	theme	model	410:414	arg1	omeprazole					421:430	The model drug omeprazole	406:430	The model drug omeprazole	406:430	The model drug omeprazole was loaded in Gel-MCN composites mainly by electrostatic interaction and hydrogen bonds, which were certified by FTIR, XRD and TGA-DSC.
28065751	0	19	theme	structural	10:19	arg1	network					39:45	Honeycomb structural composite polymer network	0:45	Honeycomb structural composite polymer network of gelatin and functional cellulose ester for controlled release of omeprazole.	0:125	Honeycomb structural composite polymer network of gelatin and functional cellulose ester for controlled release of omeprazole.
28065751	1	20	theme	Slyke	361:365	arg1	method					367:372	Van Slyke method	357:372	Van Slyke method	357:372	The functionalized cellulose ester MCN was firstly synthesized and used to cross-link gelatin by amidation between -NH2 in gelatin and active ester groups in MCN to form a composite polymer network Gel-MCN, which was confirmed by Van Slyke method, FTIR, XRD and TGA-DTG spectra.
28065751	3	21	with	anti-biodegradability	587:607	arg1	extents					704:710	different cross-linking extents	680:710	different cross-linking extents	680:710	Thermal stability, anti-biodegradability, mechanical property and surface hydrophobicity of the composites with different cross-linking extents and drug loading were systematically investigated.
28065751	3	21	with	anti-biodegradability	587:607	arg1	loading					721:727	drug loading	716:727	drug loading	716:727	Thermal stability, anti-biodegradability, mechanical property and surface hydrophobicity of the composites with different cross-linking extents and drug loading were systematically investigated.
28065751	5	22	theme	diffusion	950:958	arg1	effect					940:945	a combined effect	929:945	a combined effect of diffusion and degradation	929:974	The drug release mechanism was dominated by a combined effect of diffusion and degradation, and the release rate decreased with cross-linking degree increased.
28065751	5	23	theme	drug	889:892	arg1	mechanism					902:910	The drug release mechanism	885:910	The drug release mechanism	885:910	The drug release mechanism was dominated by a combined effect of diffusion and degradation, and the release rate decreased with cross-linking degree increased.
28065751	0	24	theme	Honeycomb	0:8	arg1	network					39:45	Honeycomb structural composite polymer network	0:45	Honeycomb structural composite polymer network of gelatin and functional cellulose ester for controlled release of omeprazole.	0:125	Honeycomb structural composite polymer network of gelatin and functional cellulose ester for controlled release of omeprazole.
28065751	4	25	theme	drug	868:871	arg1	entrapment					873:882	drug entrapment	868:882	drug entrapment	868:882	SEM images demonstrated the honeycomb structural cells of cross-linked gelatin networks and this ensured drug entrapment.
28065751	4	26	link	cross-linked	821:832	arg1	networks					842:849	cross-linked gelatin networks	821:849	cross-linked gelatin networks	821:849	SEM images demonstrated the honeycomb structural cells of cross-linked gelatin networks and this ensured drug entrapment.
28065751	0	27	theme	polymer	31:37	arg1	network					39:45	Honeycomb structural composite polymer network	0:45	Honeycomb structural composite polymer network of gelatin and functional cellulose ester for controlled release of omeprazole.	0:125	Honeycomb structural composite polymer network of gelatin and functional cellulose ester for controlled release of omeprazole.
28065751	5	28	theme	cross-linking	1013:1025	arg1	degree					1027:1032	cross-linking degree	1013:1032	cross-linking degree	1013:1032	The drug release mechanism was dominated by a combined effect of diffusion and degradation, and the release rate decreased with cross-linking degree increased.
28065751	5	29	theme	degradation	964:974	arg1	effect					940:945	a combined effect	929:945	a combined effect of diffusion and degradation	929:974	The drug release mechanism was dominated by a combined effect of diffusion and degradation, and the release rate decreased with cross-linking degree increased.
28065751	0	30	theme	composite	21:29	arg1	network					39:45	Honeycomb structural composite polymer network	0:45	Honeycomb structural composite polymer network of gelatin and functional cellulose ester for controlled release of omeprazole.	0:125	Honeycomb structural composite polymer network of gelatin and functional cellulose ester for controlled release of omeprazole.
28065751	1	31	from	groups	275:280	arg1	MCN					285:287	MCN	285:287	MCN	285:287	The functionalized cellulose ester MCN was firstly synthesized and used to cross-link gelatin by amidation between -NH2 in gelatin and active ester groups in MCN to form a composite polymer network Gel-MCN, which was confirmed by Van Slyke method, FTIR, XRD and TGA-DTG spectra.
28065751	3	32	with	property	621:628	arg1	extents					704:710	different cross-linking extents	680:710	different cross-linking extents	680:710	Thermal stability, anti-biodegradability, mechanical property and surface hydrophobicity of the composites with different cross-linking extents and drug loading were systematically investigated.
28065751	3	32	with	property	621:628	arg1	loading					721:727	drug loading	716:727	drug loading	716:727	Thermal stability, anti-biodegradability, mechanical property and surface hydrophobicity of the composites with different cross-linking extents and drug loading were systematically investigated.
28065751	4	33	theme	gelatin	834:840	arg1	networks					842:849	cross-linked gelatin networks	821:849	cross-linked gelatin networks	821:849	SEM images demonstrated the honeycomb structural cells of cross-linked gelatin networks and this ensured drug entrapment.
28065751	5	34	theme	release	894:900	arg1	mechanism					902:910	The drug release mechanism	885:910	The drug release mechanism	885:910	The drug release mechanism was dominated by a combined effect of diffusion and degradation, and the release rate decreased with cross-linking degree increased.
28065751	6	35	theme	delivery	1064:1071	arg1	system					1073:1078	The developed drug delivery system	1045:1078	The developed drug delivery system	1045:1078	The developed drug delivery system had profound significance in improving pesticide effect and bioavailability of drugs.
28065751	4	36	theme	cross-linked	821:832	arg1	networks					842:849	cross-linked gelatin networks	821:849	cross-linked gelatin networks	821:849	SEM images demonstrated the honeycomb structural cells of cross-linked gelatin networks and this ensured drug entrapment.
28065751	6	37	theme	drug	1059:1062	arg1	system					1073:1078	The developed drug delivery system	1045:1078	The developed drug delivery system	1045:1078	The developed drug delivery system had profound significance in improving pesticide effect and bioavailability of drugs.
28065751	1	38	theme	composite	299:307	arg1	Gel-MCN					325:331	a composite polymer network Gel-MCN	297:331	a composite polymer network Gel-MCN	297:331	The functionalized cellulose ester MCN was firstly synthesized and used to cross-link gelatin by amidation between -NH2 in gelatin and active ester groups in MCN to form a composite polymer network Gel-MCN, which was confirmed by Van Slyke method, FTIR, XRD and TGA-DTG spectra.
28065751	3	39	theme	mechanical	610:619	arg1	property					621:628	mechanical property	610:628	mechanical property	610:628	Thermal stability, anti-biodegradability, mechanical property and surface hydrophobicity of the composites with different cross-linking extents and drug loading were systematically investigated.
28065751	0	40	theme	gelatin	50:56	arg1	ester					83:87	gelatin and functional cellulose ester	50:87	gelatin and functional cellulose ester	50:87	Honeycomb structural composite polymer network of gelatin and functional cellulose ester for controlled release of omeprazole.
28065751	1	41	theme	polymer	309:315	arg1	Gel-MCN					325:331	a composite polymer network Gel-MCN	297:331	a composite polymer network Gel-MCN	297:331	The functionalized cellulose ester MCN was firstly synthesized and used to cross-link gelatin by amidation between -NH2 in gelatin and active ester groups in MCN to form a composite polymer network Gel-MCN, which was confirmed by Van Slyke method, FTIR, XRD and TGA-DTG spectra.
28065751	6	42	theme	profound	1084:1091	arg1	significance					1093:1104	profound significance	1084:1104	profound significance	1084:1104	The developed drug delivery system had profound significance in improving pesticide effect and bioavailability of drugs.
28065751	4	43	theme	networks	842:849	arg1	cells					812:816	the honeycomb structural cells	787:816	the honeycomb structural cells of cross-linked gelatin networks	787:849	SEM images demonstrated the honeycomb structural cells of cross-linked gelatin networks and this ensured drug entrapment.
28065751	5	44	theme	combined	931:938	arg1	effect					940:945	a combined effect	929:945	a combined effect of diffusion and degradation	929:974	The drug release mechanism was dominated by a combined effect of diffusion and degradation, and the release rate decreased with cross-linking degree increased.
28065751	1	45	theme	network	317:323	arg1	Gel-MCN					325:331	a composite polymer network Gel-MCN	297:331	a composite polymer network Gel-MCN	297:331	The functionalized cellulose ester MCN was firstly synthesized and used to cross-link gelatin by amidation between -NH2 in gelatin and active ester groups in MCN to form a composite polymer network Gel-MCN, which was confirmed by Van Slyke method, FTIR, XRD and TGA-DTG spectra.
28065751	4	46	theme	structural	801:810	arg1	cells					812:816	the honeycomb structural cells	787:816	the honeycomb structural cells of cross-linked gelatin networks	787:849	SEM images demonstrated the honeycomb structural cells of cross-linked gelatin networks and this ensured drug entrapment.
28065751	1	47	theme	functionalized	131:144	arg1	ester					156:160	The functionalized cellulose ester MCN	127:164	The functionalized cellulose ester MCN	127:164	The functionalized cellulose ester MCN was firstly synthesized and used to cross-link gelatin by amidation between -NH2 in gelatin and active ester groups in MCN to form a composite polymer network Gel-MCN, which was confirmed by Van Slyke method, FTIR, XRD and TGA-DTG spectra.
28065751	6	48	theme	developed	1049:1057	arg1	system					1073:1078	The developed drug delivery system	1045:1078	The developed drug delivery system	1045:1078	The developed drug delivery system had profound significance in improving pesticide effect and bioavailability of drugs.
28065751	1	49	from	-NH2	242:245	arg1	groups					275:280	gelatin and active ester groups	250:280	gelatin and active ester groups in MCN	250:287	The functionalized cellulose ester MCN was firstly synthesized and used to cross-link gelatin by amidation between -NH2 in gelatin and active ester groups in MCN to form a composite polymer network Gel-MCN, which was confirmed by Van Slyke method, FTIR, XRD and TGA-DTG spectra.
28065751	1	50	theme	TGA-DTG	389:395	arg1	spectra					397:403	TGA-DTG spectra	389:403	TGA-DTG spectra	389:403	The functionalized cellulose ester MCN was firstly synthesized and used to cross-link gelatin by amidation between -NH2 in gelatin and active ester groups in MCN to form a composite polymer network Gel-MCN, which was confirmed by Van Slyke method, FTIR, XRD and TGA-DTG spectra.
28065751	1	51	used	used	194:197	arg2	ester					156:160	The functionalized cellulose ester MCN	127:164	The functionalized cellulose ester MCN	127:164	The functionalized cellulose ester MCN was firstly synthesized and used to cross-link gelatin by amidation between -NH2 in gelatin and active ester groups in MCN to form a composite polymer network Gel-MCN, which was confirmed by Van Slyke method, FTIR, XRD and TGA-DTG spectra.
28065751	1	52	theme	cellulose	146:154	arg1	ester					156:160	The functionalized cellulose ester MCN	127:164	The functionalized cellulose ester MCN	127:164	The functionalized cellulose ester MCN was firstly synthesized and used to cross-link gelatin by amidation between -NH2 in gelatin and active ester groups in MCN to form a composite polymer network Gel-MCN, which was confirmed by Van Slyke method, FTIR, XRD and TGA-DTG spectra.
28065751	6	53	theme	drugs	1159:1163	arg1	bioavailability					1140:1154	bioavailability	1140:1154	bioavailability of drugs	1140:1163	The developed drug delivery system had profound significance in improving pesticide effect and bioavailability of drugs.
28065751	6	53	theme	drugs	1159:1163	arg1	effect					1129:1134	pesticide effect	1119:1134	pesticide effect	1119:1134	The developed drug delivery system had profound significance in improving pesticide effect and bioavailability of drugs.
28065751	0	54	theme	cellulose	73:81	arg1	ester					83:87	gelatin and functional cellulose ester	50:87	gelatin and functional cellulose ester	50:87	Honeycomb structural composite polymer network of gelatin and functional cellulose ester for controlled release of omeprazole.
28065751	3	55	theme	Thermal	568:574	arg1	stability					576:584	Thermal stability	568:584	Thermal stability	568:584	Thermal stability, anti-biodegradability, mechanical property and surface hydrophobicity of the composites with different cross-linking extents and drug loading were systematically investigated.
28065751	3	56	with	stability	576:584	arg1	extents					704:710	different cross-linking extents	680:710	different cross-linking extents	680:710	Thermal stability, anti-biodegradability, mechanical property and surface hydrophobicity of the composites with different cross-linking extents and drug loading were systematically investigated.
28065751	3	56	with	stability	576:584	arg1	loading					721:727	drug loading	716:727	drug loading	716:727	Thermal stability, anti-biodegradability, mechanical property and surface hydrophobicity of the composites with different cross-linking extents and drug loading were systematically investigated.
28065751	0	57	theme	functional	62:71	arg1	ester					83:87	gelatin and functional cellulose ester	50:87	gelatin and functional cellulose ester	50:87	Honeycomb structural composite polymer network of gelatin and functional cellulose ester for controlled release of omeprazole.
28065751	3	58	theme	different	680:688	arg1	extents					704:710	different cross-linking extents	680:710	different cross-linking extents	680:710	Thermal stability, anti-biodegradability, mechanical property and surface hydrophobicity of the composites with different cross-linking extents and drug loading were systematically investigated.
28065751	2	59	theme	drug	416:419	arg1	omeprazole					421:430	The model drug omeprazole	406:430	The model drug omeprazole	406:430	The model drug omeprazole was loaded in Gel-MCN composites mainly by electrostatic interaction and hydrogen bonds, which were certified by FTIR, XRD and TGA-DSC.
28065751	1	60	theme	gelatin	250:256	arg1	groups					275:280	gelatin and active ester groups	250:280	gelatin and active ester groups in MCN	250:287	The functionalized cellulose ester MCN was firstly synthesized and used to cross-link gelatin by amidation between -NH2 in gelatin and active ester groups in MCN to form a composite polymer network Gel-MCN, which was confirmed by Van Slyke method, FTIR, XRD and TGA-DTG spectra.
27196311	3	0	theme	Compositional	333:345	arg1	analyses					347:354	Compositional analyses	333:354	Compositional analyses	333:354	Compositional analyses indicated the presence of glycerol (Gro), phosphate-Gro, alanine-Gro, glucose (Glc) and fatty acids.
27196311	2	1	theme	1D	306:307	arg1	experiments					320:330	1D and 2D NMR experiments	306:330	chemical analyses as well as 1D and 2D NMR experiments	277:330	LTA isolated from allergy-protective Staphylococcus sciuri W620 strain was characterized by chemical analyses as well as 1D and 2D NMR experiments.
27196311	3	2	theme	glycerol	382:389	arg1	presence					370:377	the presence	366:377	the presence of glycerol (Gro), phosphate-Gro, alanine-Gro, glucose (Glc) and fatty acids	366:454	Compositional analyses indicated the presence of glycerol (Gro), phosphate-Gro, alanine-Gro, glucose (Glc) and fatty acids.
27196311	4	3	theme	studied	461:467	arg1	strain					469:474	The studied strain	457:474	The studied strain	457:474	The studied strain produced LTA with backbone composed of glycerol-phosphate repeating units only substituted with d-alanine (Ala) and the lipid anchor, typically for genus Staphyloccocus, possessing the structure β-d-Glcp(1→6)- β-d-Glcp(1→3)-1,2-diacyl-sn-Gro.
27196311	4	4	with	LTA	485:487	arg1	backbone					494:501	backbone	494:501	backbone composed of glycerol-phosphate repeating units only substituted with d-alanine (Ala) and the lipid anchor, typically	494:618	The studied strain produced LTA with backbone composed of glycerol-phosphate repeating units only substituted with d-alanine (Ala) and the lipid anchor, typically for genus Staphyloccocus, possessing the structure β-d-Glcp(1→6)- β-d-Glcp(1→3)-1,2-diacyl-sn-Gro.
27196311	2	5	attach	isolated	189:196	arg1	strain					249:254	allergy-protective Staphylococcus sciuri W620 strain	203:254	allergy-protective Staphylococcus sciuri W620 strain	203:254	LTA isolated from allergy-protective Staphylococcus sciuri W620 strain was characterized by chemical analyses as well as 1D and 2D NMR experiments.
27196311	2	5	attach	isolated	189:196	arg2	LTA					185:187	LTA	185:187	LTA isolated from allergy-protective Staphylococcus sciuri W620 strain	185:254	LTA isolated from allergy-protective Staphylococcus sciuri W620 strain was characterized by chemical analyses as well as 1D and 2D NMR experiments.
27196311	1	6	theme	Gram-positive	161:173	arg1	bacteria					175:182	Gram-positive bacteria	161:182	Gram-positive bacteria	161:182	Lipoteichoic acid (LTA) is an important cell envelope compound of Gram-positive bacteria.
27196311	3	7	theme	alanine-Gro	413:423	arg1	presence					370:377	the presence	366:377	the presence of glycerol (Gro), phosphate-Gro, alanine-Gro, glucose (Glc) and fatty acids	366:454	Compositional analyses indicated the presence of glycerol (Gro), phosphate-Gro, alanine-Gro, glucose (Glc) and fatty acids.
27196311	1	8	theme	bacteria	175:182	arg1	acid					108:111	Lipoteichoic acid	95:111	Lipoteichoic acid (LTA)	95:117	Lipoteichoic acid (LTA) is an important cell envelope compound of Gram-positive bacteria.
27196311	1	8	theme	bacteria	175:182	arg1	compound					149:156	an important cell envelope compound	122:156	an important cell envelope compound of Gram-positive bacteria	122:182	Lipoteichoic acid (LTA) is an important cell envelope compound of Gram-positive bacteria.
27196311	1	9	theme	Lipoteichoic	95:106	arg1	acid					108:111	Lipoteichoic acid	95:111	Lipoteichoic acid (LTA)	95:117	Lipoteichoic acid (LTA) is an important cell envelope compound of Gram-positive bacteria.
27196311	1	9	theme	Lipoteichoic	95:106	arg1	compound					149:156	an important cell envelope compound	122:156	an important cell envelope compound of Gram-positive bacteria	122:182	Lipoteichoic acid (LTA) is an important cell envelope compound of Gram-positive bacteria.
27196311	1	9	theme	Lipoteichoic	95:106	arg1	LTA					114:116	LTA	114:116	LTA	114:116	Lipoteichoic acid (LTA) is an important cell envelope compound of Gram-positive bacteria.
27196311	3	10	theme	glucose	426:432	arg1	presence					370:377	the presence	366:377	the presence of glycerol (Gro), phosphate-Gro, alanine-Gro, glucose (Glc) and fatty acids	366:454	Compositional analyses indicated the presence of glycerol (Gro), phosphate-Gro, alanine-Gro, glucose (Glc) and fatty acids.
27196311	0	11	theme	Structural	0:9	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization of the lipoteichoic acid	0:51	Structural characterization of the lipoteichoic acid isolated from Staphylococcus sciuri W620.
27196311	4	12	dep	produced	476:483	arg1	possessing					646:655	possessing	646:655	possessing the structure β-d-Glcp(1→6)- β-d-Glcp(1→3)-1,2-diacyl-sn-Gro	646:716	The studied strain produced LTA with backbone composed of glycerol-phosphate repeating units only substituted with d-alanine (Ala) and the lipid anchor, typically for genus Staphyloccocus, possessing the structure β-d-Glcp(1→6)- β-d-Glcp(1→3)-1,2-diacyl-sn-Gro.
27196311	2	13	theme	NMR	316:318	arg1	experiments					320:330	1D and 2D NMR experiments	306:330	chemical analyses as well as 1D and 2D NMR experiments	277:330	LTA isolated from allergy-protective Staphylococcus sciuri W620 strain was characterized by chemical analyses as well as 1D and 2D NMR experiments.
27196311	2	14	theme	2D	313:314	arg1	experiments					320:330	1D and 2D NMR experiments	306:330	chemical analyses as well as 1D and 2D NMR experiments	277:330	LTA isolated from allergy-protective Staphylococcus sciuri W620 strain was characterized by chemical analyses as well as 1D and 2D NMR experiments.
27196311	3	15	theme	acids	450:454	arg1	presence					370:377	the presence	366:377	the presence of glycerol (Gro), phosphate-Gro, alanine-Gro, glucose (Glc) and fatty acids	366:454	Compositional analyses indicated the presence of glycerol (Gro), phosphate-Gro, alanine-Gro, glucose (Glc) and fatty acids.
27196311	0	16	theme	acid	48:51	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization of the lipoteichoic acid	0:51	Structural characterization of the lipoteichoic acid isolated from Staphylococcus sciuri W620.
27196311	4	17	theme	structure	661:669	arg1	1→6					680:682	1→6	680:682	1→6	680:682	The studied strain produced LTA with backbone composed of glycerol-phosphate repeating units only substituted with d-alanine (Ala) and the lipid anchor, typically for genus Staphyloccocus, possessing the structure β-d-Glcp(1→6)- β-d-Glcp(1→3)-1,2-diacyl-sn-Gro.
27196311	4	17	theme	structure	661:669	arg1	β-d-Glcp					671:678	the structure β-d-Glcp	657:678	the structure β-d-Glcp(1→6)	657:683	The studied strain produced LTA with backbone composed of glycerol-phosphate repeating units only substituted with d-alanine (Ala) and the lipid anchor, typically for genus Staphyloccocus, possessing the structure β-d-Glcp(1→6)- β-d-Glcp(1→3)-1,2-diacyl-sn-Gro.
27196311	0	18	theme	lipoteichoic	35:46	arg1	acid					48:51	the lipoteichoic acid	31:51	the lipoteichoic acid	31:51	Structural characterization of the lipoteichoic acid isolated from Staphylococcus sciuri W620.
27196311	4	19	theme	glycerol-phosphate	515:532	arg1	units					544:548	glycerol-phosphate repeating units	515:548	glycerol-phosphate repeating units only substituted with d-alanine (Ala) and the lipid anchor	515:607	The studied strain produced LTA with backbone composed of glycerol-phosphate repeating units only substituted with d-alanine (Ala) and the lipid anchor, typically for genus Staphyloccocus, possessing the structure β-d-Glcp(1→6)- β-d-Glcp(1→3)-1,2-diacyl-sn-Gro.
27196311	3	20	theme	fatty	444:448	arg1	acids					450:454	fatty acids	444:454	fatty acids	444:454	Compositional analyses indicated the presence of glycerol (Gro), phosphate-Gro, alanine-Gro, glucose (Glc) and fatty acids.
27196311	3	21	theme	phosphate-Gro	398:410	arg1	presence					370:377	the presence	366:377	the presence of glycerol (Gro), phosphate-Gro, alanine-Gro, glucose (Glc) and fatty acids	366:454	Compositional analyses indicated the presence of glycerol (Gro), phosphate-Gro, alanine-Gro, glucose (Glc) and fatty acids.
27196311	2	22	theme	W620	244:247	arg1	strain					249:254	allergy-protective Staphylococcus sciuri W620 strain	203:254	allergy-protective Staphylococcus sciuri W620 strain	203:254	LTA isolated from allergy-protective Staphylococcus sciuri W620 strain was characterized by chemical analyses as well as 1D and 2D NMR experiments.
27196311	4	23	theme	β-d-Glcp	686:693	arg1	-1,2-diacyl-sn-Gro					699:716	β-d-Glcp(1→3)-1,2-diacyl-sn-Gro	686:716	β-d-Glcp(1→3)-1,2-diacyl-sn-Gro	686:716	The studied strain produced LTA with backbone composed of glycerol-phosphate repeating units only substituted with d-alanine (Ala) and the lipid anchor, typically for genus Staphyloccocus, possessing the structure β-d-Glcp(1→6)- β-d-Glcp(1→3)-1,2-diacyl-sn-Gro.
27196311	2	24	theme	Staphylococcus	222:235	arg1	strain					249:254	allergy-protective Staphylococcus sciuri W620 strain	203:254	allergy-protective Staphylococcus sciuri W620 strain	203:254	LTA isolated from allergy-protective Staphylococcus sciuri W620 strain was characterized by chemical analyses as well as 1D and 2D NMR experiments.
27196311	2	25	theme	sciuri	237:242	arg1	strain					249:254	allergy-protective Staphylococcus sciuri W620 strain	203:254	allergy-protective Staphylococcus sciuri W620 strain	203:254	LTA isolated from allergy-protective Staphylococcus sciuri W620 strain was characterized by chemical analyses as well as 1D and 2D NMR experiments.
27196311	4	26	theme	repeating	534:542	arg1	units					544:548	glycerol-phosphate repeating units	515:548	glycerol-phosphate repeating units only substituted with d-alanine (Ala) and the lipid anchor	515:607	The studied strain produced LTA with backbone composed of glycerol-phosphate repeating units only substituted with d-alanine (Ala) and the lipid anchor, typically for genus Staphyloccocus, possessing the structure β-d-Glcp(1→6)- β-d-Glcp(1→3)-1,2-diacyl-sn-Gro.
27196311	2	27	theme	allergy-protective	203:220	arg1	strain					249:254	allergy-protective Staphylococcus sciuri W620 strain	203:254	allergy-protective Staphylococcus sciuri W620 strain	203:254	LTA isolated from allergy-protective Staphylococcus sciuri W620 strain was characterized by chemical analyses as well as 1D and 2D NMR experiments.
27196311	4	28	theme	1→3	695:697	arg1	-1,2-diacyl-sn-Gro					699:716	β-d-Glcp(1→3)-1,2-diacyl-sn-Gro	686:716	β-d-Glcp(1→3)-1,2-diacyl-sn-Gro	686:716	The studied strain produced LTA with backbone composed of glycerol-phosphate repeating units only substituted with d-alanine (Ala) and the lipid anchor, typically for genus Staphyloccocus, possessing the structure β-d-Glcp(1→6)- β-d-Glcp(1→3)-1,2-diacyl-sn-Gro.
27196311	4	29	theme	lipid	596:600	arg1	anchor					602:607	the lipid anchor	592:607	the lipid anchor	592:607	The studied strain produced LTA with backbone composed of glycerol-phosphate repeating units only substituted with d-alanine (Ala) and the lipid anchor, typically for genus Staphyloccocus, possessing the structure β-d-Glcp(1→6)- β-d-Glcp(1→3)-1,2-diacyl-sn-Gro.
27196311	2	30	theme	chemical	277:284	arg1	analyses					286:293	chemical analyses	277:293	chemical analyses as well as 1D and 2D NMR experiments	277:330	LTA isolated from allergy-protective Staphylococcus sciuri W620 strain was characterized by chemical analyses as well as 1D and 2D NMR experiments.
27196311	1	31	theme	important	125:133	arg1	acid					108:111	Lipoteichoic acid	95:111	Lipoteichoic acid (LTA)	95:117	Lipoteichoic acid (LTA) is an important cell envelope compound of Gram-positive bacteria.
27196311	1	31	theme	important	125:133	arg1	compound					149:156	an important cell envelope compound	122:156	an important cell envelope compound of Gram-positive bacteria	122:182	Lipoteichoic acid (LTA) is an important cell envelope compound of Gram-positive bacteria.
27196311	0	32	theme	sciuri	82:87	arg1	W620					89:92	Staphylococcus sciuri W620	67:92	Staphylococcus sciuri W620	67:92	Structural characterization of the lipoteichoic acid isolated from Staphylococcus sciuri W620.
27196311	1	33	theme	cell	135:138	arg1	acid					108:111	Lipoteichoic acid	95:111	Lipoteichoic acid (LTA)	95:117	Lipoteichoic acid (LTA) is an important cell envelope compound of Gram-positive bacteria.
27196311	1	33	theme	cell	135:138	arg1	compound					149:156	an important cell envelope compound	122:156	an important cell envelope compound of Gram-positive bacteria	122:182	Lipoteichoic acid (LTA) is an important cell envelope compound of Gram-positive bacteria.
27196311	0	34	theme	Staphylococcus	67:80	arg1	W620					89:92	Staphylococcus sciuri W620	67:92	Staphylococcus sciuri W620	67:92	Structural characterization of the lipoteichoic acid isolated from Staphylococcus sciuri W620.
27196311	1	35	theme	envelope	140:147	arg1	acid					108:111	Lipoteichoic acid	95:111	Lipoteichoic acid (LTA)	95:117	Lipoteichoic acid (LTA) is an important cell envelope compound of Gram-positive bacteria.
27196311	1	35	theme	envelope	140:147	arg1	compound					149:156	an important cell envelope compound	122:156	an important cell envelope compound of Gram-positive bacteria	122:182	Lipoteichoic acid (LTA) is an important cell envelope compound of Gram-positive bacteria.
27106158	5	0	dep	In	696:697	arg1	vitro					699:703	vitro	699:703	vitro	699:703	In vitro antibacterial assay demonstrate that the developed BC-TCH composites displayed excellent antibacterial activity solely associated with the loaded TCH drug.
27106158	1	1	theme	biomedical	167:176	arg1	applications					178:189	biomedical applications	167:189	biomedical applications	167:189	Bacterial cellulose (BC) is widely used in biomedical applications.
27106158	4	2	theme	release	595:601	arg1	results					603:609	The TCH release results	587:609	The TCH release results	587:609	The TCH release results show that the incorporation of BC matrix to load TCH is able to control the release.
27106158	0	3	theme	composite	103:111	arg1	membranes					113:121	bacterial cellulose composite membranes	83:121	bacterial cellulose composite membranes	83:121	Controlled release and antibacterial activity of tetracycline hydrochloride-loaded bacterial cellulose composite membranes.
27106158	4	4	theme	TCH	591:593	arg1	results					603:609	The TCH release results	587:609	The TCH release results	587:609	The TCH release results show that the incorporation of BC matrix to load TCH is able to control the release.
27106158	6	5	theme	BC-TCH	883:888	arg1	membranes					900:908	the BC-TCH composite membranes	879:908	the BC-TCH composite membranes	879:908	More importantly, the BC-TCH composite membranes display good biocompatibility.
27106158	2	6	theme	BC	299:300	arg1	membranes					313:321	an antibiotic drug tetracycline hydrochloride (TCH)-loaded bacterial cellulose (BC) composite membranes	219:321	an antibiotic drug tetracycline hydrochloride (TCH)-loaded bacterial cellulose (BC) composite membranes	219:321	In this study, we prepared an antibiotic drug tetracycline hydrochloride (TCH)-loaded bacterial cellulose (BC) composite membranes, and evaluated the drug release, antibacterial activity and biocompatibility.
27106158	3	7	dep	transform	547:555	arg1	infrared					557:564	infrared	557:564	transform infrared spectroscopy (FTIR)	547:584	The structure and morphology of the fabricated BC-TCH composite membranes were characterized using scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR).
27106158	3	8	dep	structure	405:413	arg1	The					401:403	The	401:403	The	401:403	The structure and morphology of the fabricated BC-TCH composite membranes were characterized using scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR).
27106158	2	9	theme	cellulose	288:296	arg1	membranes					313:321	an antibiotic drug tetracycline hydrochloride (TCH)-loaded bacterial cellulose (BC) composite membranes	219:321	an antibiotic drug tetracycline hydrochloride (TCH)-loaded bacterial cellulose (BC) composite membranes	219:321	In this study, we prepared an antibiotic drug tetracycline hydrochloride (TCH)-loaded bacterial cellulose (BC) composite membranes, and evaluated the drug release, antibacterial activity and biocompatibility.
27106158	3	10	theme	BC-TCH	448:453	arg1	membranes					465:473	the fabricated BC-TCH composite membranes	433:473	the fabricated BC-TCH composite membranes	433:473	The structure and morphology of the fabricated BC-TCH composite membranes were characterized using scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR).
27106158	5	11	theme	developed	746:754	arg1	composites					763:772	the developed BC-TCH composites	742:772	the developed BC-TCH composites	742:772	In vitro antibacterial assay demonstrate that the developed BC-TCH composites displayed excellent antibacterial activity solely associated with the loaded TCH drug.
27106158	7	12	theme	membranes	983:991	arg1	characteristics					947:961	These characteristics	941:961	These characteristics of BC-TCH composite membranes	941:991	These characteristics of BC-TCH composite membranes indicate that they may successfully serve as wound dressings and other medical biomaterials.
27106158	2	13	theme	bacterial	278:286	arg1	membranes					313:321	an antibiotic drug tetracycline hydrochloride (TCH)-loaded bacterial cellulose (BC) composite membranes	219:321	an antibiotic drug tetracycline hydrochloride (TCH)-loaded bacterial cellulose (BC) composite membranes	219:321	In this study, we prepared an antibiotic drug tetracycline hydrochloride (TCH)-loaded bacterial cellulose (BC) composite membranes, and evaluated the drug release, antibacterial activity and biocompatibility.
27106158	3	14	theme	composite	455:463	arg1	membranes					465:473	the fabricated BC-TCH composite membranes	433:473	the fabricated BC-TCH composite membranes	433:473	The structure and morphology of the fabricated BC-TCH composite membranes were characterized using scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR).
27106158	5	15	theme	excellent	784:792	arg1	activity					808:815	excellent antibacterial activity	784:815	excellent antibacterial activity solely associated with the loaded TCH drug	784:858	In vitro antibacterial assay demonstrate that the developed BC-TCH composites displayed excellent antibacterial activity solely associated with the loaded TCH drug.
27106158	5	16	theme	BC-TCH	756:761	arg1	composites					763:772	the developed BC-TCH composites	742:772	the developed BC-TCH composites	742:772	In vitro antibacterial assay demonstrate that the developed BC-TCH composites displayed excellent antibacterial activity solely associated with the loaded TCH drug.
27106158	6	17	theme	good	918:921	arg1	biocompatibility					923:938	good biocompatibility	918:938	good biocompatibility	918:938	More importantly, the BC-TCH composite membranes display good biocompatibility.
27106158	0	18	theme	Controlled	0:9	arg1	release					11:17	Controlled release	0:17	Controlled release	0:17	Controlled release and antibacterial activity of tetracycline hydrochloride-loaded bacterial cellulose composite membranes.
27106158	7	19	theme	BC-TCH	966:971	arg1	membranes					983:991	BC-TCH composite membranes	966:991	BC-TCH composite membranes	966:991	These characteristics of BC-TCH composite membranes indicate that they may successfully serve as wound dressings and other medical biomaterials.
27106158	1	20	theme	Bacterial	124:132	arg1	BC					145:146	BC	145:146	BC	145:146	Bacterial cellulose (BC) is widely used in biomedical applications.
27106158	1	20	theme	Bacterial	124:132	arg1	cellulose					134:142	Bacterial cellulose	124:142	Bacterial cellulose (BC)	124:147	Bacterial cellulose (BC) is widely used in biomedical applications.
27106158	3	21	theme	fabricated	437:446	arg1	membranes					465:473	the fabricated BC-TCH composite membranes	433:473	the fabricated BC-TCH composite membranes	433:473	The structure and morphology of the fabricated BC-TCH composite membranes were characterized using scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR).
27106158	0	22	theme	antibacterial	23:35	arg1	activity					37:44	antibacterial activity	23:44	antibacterial activity	23:44	Controlled release and antibacterial activity of tetracycline hydrochloride-loaded bacterial cellulose composite membranes.
27106158	5	23	theme	In	696:697	arg1	assay					719:723	In vitro antibacterial assay	696:723	In vitro antibacterial assay	696:723	In vitro antibacterial assay demonstrate that the developed BC-TCH composites displayed excellent antibacterial activity solely associated with the loaded TCH drug.
27106158	7	24	theme	composite	973:981	arg1	membranes					983:991	BC-TCH composite membranes	966:991	BC-TCH composite membranes	966:991	These characteristics of BC-TCH composite membranes indicate that they may successfully serve as wound dressings and other medical biomaterials.
27106158	2	25	theme	composite	303:311	arg1	membranes					313:321	an antibiotic drug tetracycline hydrochloride (TCH)-loaded bacterial cellulose (BC) composite membranes	219:321	an antibiotic drug tetracycline hydrochloride (TCH)-loaded bacterial cellulose (BC) composite membranes	219:321	In this study, we prepared an antibiotic drug tetracycline hydrochloride (TCH)-loaded bacterial cellulose (BC) composite membranes, and evaluated the drug release, antibacterial activity and biocompatibility.
27106158	4	26	theme	matrix	645:650	arg1	able					667:670	able	667:670	able	667:670	The TCH release results show that the incorporation of BC matrix to load TCH is able to control the release.
27106158	4	26	theme	matrix	645:650	arg1	incorporation					625:637	the incorporation	621:637	the incorporation of BC matrix to load TCH	621:662	The TCH release results show that the incorporation of BC matrix to load TCH is able to control the release.
27106158	5	27	theme	TCH	851:853	arg1	drug					855:858	the loaded TCH drug	840:858	the loaded TCH drug	840:858	In vitro antibacterial assay demonstrate that the developed BC-TCH composites displayed excellent antibacterial activity solely associated with the loaded TCH drug.
27106158	6	28	theme	composite	890:898	arg1	membranes					900:908	the BC-TCH composite membranes	879:908	the BC-TCH composite membranes	879:908	More importantly, the BC-TCH composite membranes display good biocompatibility.
27106158	3	29	dep	Fourier	539:545	arg1	transform					547:555	transform	547:555	transform infrared spectroscopy (FTIR)	547:584	The structure and morphology of the fabricated BC-TCH composite membranes were characterized using scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR).
27106158	4	30	theme	BC	642:643	arg1	matrix					645:650	BC matrix	642:650	BC matrix	642:650	The TCH release results show that the incorporation of BC matrix to load TCH is able to control the release.
27106158	5	31	theme	antibacterial	794:806	arg1	activity					808:815	excellent antibacterial activity	784:815	excellent antibacterial activity solely associated with the loaded TCH drug	784:858	In vitro antibacterial assay demonstrate that the developed BC-TCH composites displayed excellent antibacterial activity solely associated with the loaded TCH drug.
27106158	5	32	theme	antibacterial	705:717	arg1	assay					719:723	In vitro antibacterial assay	696:723	In vitro antibacterial assay	696:723	In vitro antibacterial assay demonstrate that the developed BC-TCH composites displayed excellent antibacterial activity solely associated with the loaded TCH drug.
27106158	4	33	theme	load	655:658	arg1	TCH					660:662	load TCH	655:662	load TCH	655:662	The TCH release results show that the incorporation of BC matrix to load TCH is able to control the release.
27106158	3	34	theme	membranes	465:473	arg1	morphology					419:428	morphology	419:428	morphology	419:428	The structure and morphology of the fabricated BC-TCH composite membranes were characterized using scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR).
27106158	3	34	theme	membranes	465:473	arg1	structure					405:413	structure	405:413	structure	405:413	The structure and morphology of the fabricated BC-TCH composite membranes were characterized using scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR).
27106158	5	35	theme	loaded	844:849	arg1	drug					855:858	the loaded TCH drug	840:858	the loaded TCH drug	840:858	In vitro antibacterial assay demonstrate that the developed BC-TCH composites displayed excellent antibacterial activity solely associated with the loaded TCH drug.
27106158	7	36	theme	medical	1064:1070	arg1	they					1007:1010	they	1007:1010	they	1007:1010	These characteristics of BC-TCH composite membranes indicate that they may successfully serve as wound dressings and other medical biomaterials.
27106158	7	36	theme	medical	1064:1070	arg1	biomaterials					1072:1083	other medical biomaterials	1058:1083	other medical biomaterials	1058:1083	These characteristics of BC-TCH composite membranes indicate that they may successfully serve as wound dressings and other medical biomaterials.
27106158	2	37	theme	drug	342:345	arg1	release					347:353	the drug release	338:353	the drug release	338:353	In this study, we prepared an antibiotic drug tetracycline hydrochloride (TCH)-loaded bacterial cellulose (BC) composite membranes, and evaluated the drug release, antibacterial activity and biocompatibility.
27106158	0	38	theme	tetracycline	49:60	arg1	release					11:17	Controlled release	0:17	Controlled release	0:17	Controlled release and antibacterial activity of tetracycline hydrochloride-loaded bacterial cellulose composite membranes.
27106158	0	38	theme	tetracycline	49:60	arg1	activity					37:44	antibacterial activity	23:44	antibacterial activity	23:44	Controlled release and antibacterial activity of tetracycline hydrochloride-loaded bacterial cellulose composite membranes.
27106158	2	39	theme	antibiotic	222:231	arg1	membranes					313:321	an antibiotic drug tetracycline hydrochloride (TCH)-loaded bacterial cellulose (BC) composite membranes	219:321	an antibiotic drug tetracycline hydrochloride (TCH)-loaded bacterial cellulose (BC) composite membranes	219:321	In this study, we prepared an antibiotic drug tetracycline hydrochloride (TCH)-loaded bacterial cellulose (BC) composite membranes, and evaluated the drug release, antibacterial activity and biocompatibility.
27106158	2	40	theme	-loaded	270:276	arg1	membranes					313:321	an antibiotic drug tetracycline hydrochloride (TCH)-loaded bacterial cellulose (BC) composite membranes	219:321	an antibiotic drug tetracycline hydrochloride (TCH)-loaded bacterial cellulose (BC) composite membranes	219:321	In this study, we prepared an antibiotic drug tetracycline hydrochloride (TCH)-loaded bacterial cellulose (BC) composite membranes, and evaluated the drug release, antibacterial activity and biocompatibility.
27106158	7	41	theme	other	1058:1062	arg1	they					1007:1010	they	1007:1010	they	1007:1010	These characteristics of BC-TCH composite membranes indicate that they may successfully serve as wound dressings and other medical biomaterials.
27106158	7	41	theme	other	1058:1062	arg1	biomaterials					1072:1083	other medical biomaterials	1058:1083	other medical biomaterials	1058:1083	These characteristics of BC-TCH composite membranes indicate that they may successfully serve as wound dressings and other medical biomaterials.
27106158	3	42	theme	scanning	500:507	arg1	microscopy					518:527	scanning electron microscopy	500:527	scanning electron microscopy (SEM)	500:533	The structure and morphology of the fabricated BC-TCH composite membranes were characterized using scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR).
27106158	3	42	theme	scanning	500:507	arg1	SEM					530:532	SEM	530:532	SEM	530:532	The structure and morphology of the fabricated BC-TCH composite membranes were characterized using scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR).
27106158	1	43	used	used	159:162	arg2	BC					145:146	BC	145:146	BC	145:146	Bacterial cellulose (BC) is widely used in biomedical applications.
27106158	1	43	used	used	159:162	arg2	cellulose					134:142	Bacterial cellulose	124:142	Bacterial cellulose (BC)	124:147	Bacterial cellulose (BC) is widely used in biomedical applications.
27106158	7	44	theme	wound	1038:1042	arg1	they					1007:1010	they	1007:1010	they	1007:1010	These characteristics of BC-TCH composite membranes indicate that they may successfully serve as wound dressings and other medical biomaterials.
27106158	7	44	theme	wound	1038:1042	arg1	dressings					1044:1052	wound dressings	1038:1052	wound dressings	1038:1052	These characteristics of BC-TCH composite membranes indicate that they may successfully serve as wound dressings and other medical biomaterials.
27106158	3	45	theme	electron	509:516	arg1	microscopy					518:527	scanning electron microscopy	500:527	scanning electron microscopy (SEM)	500:533	The structure and morphology of the fabricated BC-TCH composite membranes were characterized using scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR).
27106158	3	45	theme	electron	509:516	arg1	SEM					530:532	SEM	530:532	SEM	530:532	The structure and morphology of the fabricated BC-TCH composite membranes were characterized using scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR).
27106158	0	46	theme	cellulose	93:101	arg1	membranes					113:121	bacterial cellulose composite membranes	83:121	bacterial cellulose composite membranes	83:121	Controlled release and antibacterial activity of tetracycline hydrochloride-loaded bacterial cellulose composite membranes.
27106158	0	47	theme	bacterial	83:91	arg1	membranes					113:121	bacterial cellulose composite membranes	83:121	bacterial cellulose composite membranes	83:121	Controlled release and antibacterial activity of tetracycline hydrochloride-loaded bacterial cellulose composite membranes.
27106158	2	48	theme	antibacterial	356:368	arg1	activity					370:377	antibacterial activity	356:377	antibacterial activity	356:377	In this study, we prepared an antibiotic drug tetracycline hydrochloride (TCH)-loaded bacterial cellulose (BC) composite membranes, and evaluated the drug release, antibacterial activity and biocompatibility.
26942653	14	0	theme	healing	1720:1726	arg1	stages					1710:1715	advanced stages	1701:1715	advanced stages of healing	1701:1726	Histopathological examination revealed advanced stages of healing in treated femora compared to control ones.
26942653	8	1	theme	chitosan/anionic	980:995	arg1	combinations					997:1008	all chitosan/anionic combinations	976:1008	all chitosan/anionic combinations	976:1008	Polyelectrolyte complex formation was proved by DSC and FT-IR for all chitosan/anionic combinations except with xanthan gum where chitosan probably bound to the drug rather than xanthan gum.
26942653	1	2	theme	healing	166:172	arg1	properties					174:183	potential bone healing properties	151:183	potential bone healing properties	151:183	OBJECTIVE Statins proved potential bone healing properties.
26942653	14	3	theme	advanced	1701:1708	arg1	stages					1710:1715	advanced stages	1701:1715	advanced stages of healing	1701:1726	Histopathological examination revealed advanced stages of healing in treated femora compared to control ones.
26942653	12	4	theme	further	1571:1577	arg1	optimization					1587:1598	further release optimization	1571:1598	further release optimization	1571:1598	Imwitor® 900K and HPMC K100M were added as release retarardants for further release optimization.
26942653	4	5	theme	factorial	548:556	arg1	design					558:563	a 41.31 full factorial design	535:563	a 41.31 full factorial design	535:563	METHOD Twelve lyophilized sponge formulae were prepared adapting a 41.31 full factorial design.
26942653	15	6	theme	enhanced	1858:1865	arg1	properties					1898:1907	significantly enhanced wound healing and regeneration properties	1844:1907	significantly enhanced wound healing and regeneration properties to fractured bones	1844:1926	CONCLUSION Biodegradable sponges for local rosuvastatin delivery proved significantly enhanced wound healing and regeneration properties to fractured bones.
26942653	4	7	theme	lyophilized	484:494	arg1	formulae					503:510	METHOD Twelve lyophilized sponge formulae	470:510	METHOD Twelve lyophilized sponge formulae	470:510	METHOD Twelve lyophilized sponge formulae were prepared adapting a 41.31 full factorial design.
26942653	7	8	theme	bending	893:899	arg1	ability					901:907	considerable bending ability	880:907	considerable bending ability	880:907	RESULTS Visual and microscopic examination showed flexible homogenous porous structures with considerable bending ability.
26942653	4	9	theme	full	543:546	arg1	design					558:563	a 41.31 full factorial design	535:563	a 41.31 full factorial design	535:563	METHOD Twelve lyophilized sponge formulae were prepared adapting a 41.31 full factorial design.
26942653	5	10	theme	anionic	686:692	arg1	ratios					702:707	anionic polymer ratios	686:707	anionic polymer ratios (1:3, 1:1, 3:1)	686:723	Xanthan gum, polycarbophil, Carbopol® and sodium alginate were investigated as anionic polymers, each at three chitosan:anionic polymer ratios (1:3, 1:1, 3:1).
26942653	15	11	theme	CONCLUSION	1772:1781	arg1	sponges					1797:1803	CONCLUSION Biodegradable sponges	1772:1803	CONCLUSION Biodegradable sponges for local rosuvastatin delivery	1772:1835	CONCLUSION Biodegradable sponges for local rosuvastatin delivery proved significantly enhanced wound healing and regeneration properties to fractured bones.
26942653	13	12	theme	fractured	1640:1648	arg1	femora					1654:1659	fractured rat femora	1640:1659	fractured rat femora	1640:1659	The formula of choice was implanted in fractured rat femora.
26942653	9	13	dep	polymer	1142:1148	arg1	chitosan					1159:1166	chitosan	1159:1166	chitosan	1159:1166	Statistical analysis proved that anionic polymer type and chitosan: polymer ratio, as well as, their interactions, exhibited significant effects on the release parameters at p ≤ 0.05.
26942653	9	13	dep	polymer	1142:1148	arg1	ratio					1177:1181	polymer ratio	1169:1181	polymer ratio	1169:1181	Statistical analysis proved that anionic polymer type and chitosan: polymer ratio, as well as, their interactions, exhibited significant effects on the release parameters at p ≤ 0.05.
26942653	9	13	dep	polymer	1142:1148	arg1	type					1150:1153	type	1150:1153	type	1150:1153	Statistical analysis proved that anionic polymer type and chitosan: polymer ratio, as well as, their interactions, exhibited significant effects on the release parameters at p ≤ 0.05.
26942653	0	14	theme	bone	95:98	arg1	healing					100:106	bone healing	95:106	bone healing	95:106	Localized rosuvastatin via implantable bioerodible sponge and its potential role in augmenting bone healing and regeneration.
26942653	12	15	theme	release	1546:1552	arg1	K100M					1526:1530	HPMC K100M	1521:1530	HPMC K100M	1521:1530	Imwitor® 900K and HPMC K100M were added as release retarardants for further release optimization.
26942653	12	15	theme	release	1546:1552	arg1	900K					1512:1515	Imwitor® 900K	1503:1515	Imwitor® 900K	1503:1515	Imwitor® 900K and HPMC K100M were added as release retarardants for further release optimization.
26942653	12	15	theme	release	1546:1552	arg1	retarardants					1554:1565	release retarardants	1546:1565	release retarardants for further release optimization	1546:1598	Imwitor® 900K and HPMC K100M were added as release retarardants for further release optimization.
26942653	5	16	theme	polymer	694:700	arg1	ratios					702:707	anionic polymer ratios	686:707	anionic polymer ratios (1:3, 1:1, 3:1)	686:723	Xanthan gum, polycarbophil, Carbopol® and sodium alginate were investigated as anionic polymers, each at three chitosan:anionic polymer ratios (1:3, 1:1, 3:1).
26942653	5	17	theme	anionic	645:651	arg1	alginate					615:622	sodium alginate	608:622	sodium alginate	608:622	Xanthan gum, polycarbophil, Carbopol® and sodium alginate were investigated as anionic polymers, each at three chitosan:anionic polymer ratios (1:3, 1:1, 3:1).
26942653	5	17	theme	anionic	645:651	arg1	each					663:666	each	663:666	each	663:666	Xanthan gum, polycarbophil, Carbopol® and sodium alginate were investigated as anionic polymers, each at three chitosan:anionic polymer ratios (1:3, 1:1, 3:1).
26942653	5	17	theme	anionic	645:651	arg1	polycarbophil					579:591	polycarbophil	579:591	polycarbophil	579:591	Xanthan gum, polycarbophil, Carbopol® and sodium alginate were investigated as anionic polymers, each at three chitosan:anionic polymer ratios (1:3, 1:1, 3:1).
26942653	5	17	theme	anionic	645:651	arg1	Carbopol®					594:602	Carbopol®	594:602	Carbopol®	594:602	Xanthan gum, polycarbophil, Carbopol® and sodium alginate were investigated as anionic polymers, each at three chitosan:anionic polymer ratios (1:3, 1:1, 3:1).
26942653	5	17	theme	anionic	645:651	arg1	polymers					653:660	anionic polymers	645:660	anionic polymers	645:660	Xanthan gum, polycarbophil, Carbopol® and sodium alginate were investigated as anionic polymers, each at three chitosan:anionic polymer ratios (1:3, 1:1, 3:1).
26942653	5	17	theme	anionic	645:651	arg1	gum					574:576	Xanthan gum	566:576	Xanthan gum	566:576	Xanthan gum, polycarbophil, Carbopol® and sodium alginate were investigated as anionic polymers, each at three chitosan:anionic polymer ratios (1:3, 1:1, 3:1).
26942653	13	18	theme	rat	1650:1652	arg1	femora					1654:1659	fractured rat femora	1640:1659	fractured rat femora	1640:1659	The formula of choice was implanted in fractured rat femora.
26942653	9	19	theme	Statistical	1101:1111	arg1	analysis					1113:1120	Statistical analysis	1101:1120	Statistical analysis	1101:1120	Statistical analysis proved that anionic polymer type and chitosan: polymer ratio, as well as, their interactions, exhibited significant effects on the release parameters at p ≤ 0.05.
26942653	12	20	theme	release	1579:1585	arg1	optimization					1587:1598	further release optimization	1571:1598	further release optimization	1571:1598	Imwitor® 900K and HPMC K100M were added as release retarardants for further release optimization.
26942653	3	21	theme	bioerodible	344:354	arg1	sponges					366:372	various bioerodible composite sponges	336:372	various bioerodible composite sponges enclosing rosuvastatin	336:395	In the current work, an attempt was investigated to develop, evaluate various bioerodible composite sponges enclosing rosuvastatin and explore their potential in augmenting bone healing and regeneration.
26942653	8	22	theme	xanthan	1088:1094	arg1	gum					1096:1098	xanthan gum	1088:1098	the drug rather than xanthan gum	1067:1098	Polyelectrolyte complex formation was proved by DSC and FT-IR for all chitosan/anionic combinations except with xanthan gum where chitosan probably bound to the drug rather than xanthan gum.
26942653	8	23	theme	xanthan	1022:1028	arg1	gum					1030:1032	xanthan gum	1022:1032	xanthan gum	1022:1032	Polyelectrolyte complex formation was proved by DSC and FT-IR for all chitosan/anionic combinations except with xanthan gum where chitosan probably bound to the drug rather than xanthan gum.
26942653	7	24	theme	considerable	880:891	arg1	ability					901:907	considerable bending ability	880:907	considerable bending ability	880:907	RESULTS Visual and microscopic examination showed flexible homogenous porous structures with considerable bending ability.
26942653	3	25	theme	composite	356:364	arg1	sponges					366:372	various bioerodible composite sponges	336:372	various bioerodible composite sponges enclosing rosuvastatin	336:395	In the current work, an attempt was investigated to develop, evaluate various bioerodible composite sponges enclosing rosuvastatin and explore their potential in augmenting bone healing and regeneration.
26942653	9	26	theme	significant	1226:1236	arg1	effects					1238:1244	significant effects	1226:1244	significant effects on the release parameters	1226:1270	Statistical analysis proved that anionic polymer type and chitosan: polymer ratio, as well as, their interactions, exhibited significant effects on the release parameters at p ≤ 0.05.
26942653	15	27	theme	healing	1873:1879	arg1	properties					1898:1907	significantly enhanced wound healing and regeneration properties	1844:1907	significantly enhanced wound healing and regeneration properties to fractured bones	1844:1926	CONCLUSION Biodegradable sponges for local rosuvastatin delivery proved significantly enhanced wound healing and regeneration properties to fractured bones.
26942653	7	28	theme	porous	857:862	arg1	structures					864:873	flexible homogenous porous structures	837:873	flexible homogenous porous structures	837:873	RESULTS Visual and microscopic examination showed flexible homogenous porous structures with considerable bending ability.
26942653	5	29	dep	ratios	702:707	arg1	1:3					710:712	1:3	710:712	1:3	710:712	Xanthan gum, polycarbophil, Carbopol® and sodium alginate were investigated as anionic polymers, each at three chitosan:anionic polymer ratios (1:3, 1:1, 3:1).
26942653	5	29	dep	ratios	702:707	arg1	3:1					720:722	3:1	720:722	3:1	720:722	Xanthan gum, polycarbophil, Carbopol® and sodium alginate were investigated as anionic polymers, each at three chitosan:anionic polymer ratios (1:3, 1:1, 3:1).
26942653	5	29	dep	ratios	702:707	arg1	1:1					715:717	1:1	715:717	1:1	715:717	Xanthan gum, polycarbophil, Carbopol® and sodium alginate were investigated as anionic polymers, each at three chitosan:anionic polymer ratios (1:3, 1:1, 3:1).
26942653	15	30	theme	Biodegradable	1783:1795	arg1	sponges					1797:1803	CONCLUSION Biodegradable sponges	1772:1803	CONCLUSION Biodegradable sponges for local rosuvastatin delivery	1772:1835	CONCLUSION Biodegradable sponges for local rosuvastatin delivery proved significantly enhanced wound healing and regeneration properties to fractured bones.
26942653	11	31	contain	had	1478:1480	arg1	ratios					1471:1476	other ratios	1465:1476	other ratios	1465:1476	The release at these ratios followed Fiction diffusion while other ratios had anomalous diffusion.
26942653	11	31	contain	had	1478:1480	arg2	diffusion					1492:1500	anomalous diffusion	1482:1500	anomalous diffusion	1482:1500	The release at these ratios followed Fiction diffusion while other ratios had anomalous diffusion.
26942653	5	32	theme	sodium	608:613	arg1	alginate					615:622	sodium alginate	608:622	sodium alginate	608:622	Xanthan gum, polycarbophil, Carbopol® and sodium alginate were investigated as anionic polymers, each at three chitosan:anionic polymer ratios (1:3, 1:1, 3:1).
26942653	5	32	theme	sodium	608:613	arg1	polycarbophil					579:591	polycarbophil	579:591	polycarbophil	579:591	Xanthan gum, polycarbophil, Carbopol® and sodium alginate were investigated as anionic polymers, each at three chitosan:anionic polymer ratios (1:3, 1:1, 3:1).
26942653	5	32	theme	sodium	608:613	arg1	gum					574:576	Xanthan gum	566:576	Xanthan gum	566:576	Xanthan gum, polycarbophil, Carbopol® and sodium alginate were investigated as anionic polymers, each at three chitosan:anionic polymer ratios (1:3, 1:1, 3:1).
26942653	5	32	theme	sodium	608:613	arg1	Carbopol®					594:602	Carbopol®	594:602	Carbopol®	594:602	Xanthan gum, polycarbophil, Carbopol® and sodium alginate were investigated as anionic polymers, each at three chitosan:anionic polymer ratios (1:3, 1:1, 3:1).
26942653	5	32	theme	sodium	608:613	arg1	polymers					653:660	anionic polymers	645:660	anionic polymers	645:660	Xanthan gum, polycarbophil, Carbopol® and sodium alginate were investigated as anionic polymers, each at three chitosan:anionic polymer ratios (1:3, 1:1, 3:1).
26942653	13	33	theme	choice	1616:1621	arg1	formula					1605:1611	The formula	1601:1611	The formula of choice	1601:1621	The formula of choice was implanted in fractured rat femora.
26942653	10	34	theme	chitosan/anionic	1297:1312	arg1	3:1					1347:1349	3:1	1347:1349	3:1	1347:1349	The optimum chitosan/anionic polymer complexation ratios were 3:1 for polycarbophil and 1:1 for Carbopol and alginate.
26942653	10	34	theme	chitosan/anionic	1297:1312	arg1	ratios					1335:1340	The optimum chitosan/anionic polymer complexation ratios	1285:1340	The optimum chitosan/anionic polymer complexation ratios	1285:1340	The optimum chitosan/anionic polymer complexation ratios were 3:1 for polycarbophil and 1:1 for Carbopol and alginate.
26942653	15	35	theme	local	1809:1813	arg1	delivery					1828:1835	local rosuvastatin delivery	1809:1835	local rosuvastatin delivery	1809:1835	CONCLUSION Biodegradable sponges for local rosuvastatin delivery proved significantly enhanced wound healing and regeneration properties to fractured bones.
26942653	0	36	theme	Localized	0:8	arg1	rosuvastatin					10:21	Localized rosuvastatin	0:21	Localized rosuvastatin via implantable bioerodible sponge	0:56	Localized rosuvastatin via implantable bioerodible sponge and its potential role in augmenting bone healing and regeneration.
26942653	15	37	theme	fractured	1912:1920	arg1	bones					1922:1926	fractured bones	1912:1926	fractured bones	1912:1926	CONCLUSION Biodegradable sponges for local rosuvastatin delivery proved significantly enhanced wound healing and regeneration properties to fractured bones.
26942653	11	38	from	ratios	1425:1430	arg1	release					1408:1414	The release	1404:1414	The release at these ratios	1404:1430	The release at these ratios followed Fiction diffusion while other ratios had anomalous diffusion.
26942653	6	39	theme	rat	775:777	arg1	femora					779:784	fractured rat femora	765:784	fractured rat femora	765:784	The formula of choice was implanted in fractured rat femora.
26942653	7	40	theme	flexible	837:844	arg1	structures					864:873	flexible homogenous porous structures	837:873	flexible homogenous porous structures	837:873	RESULTS Visual and microscopic examination showed flexible homogenous porous structures with considerable bending ability.
26942653	9	41	theme	release	1253:1259	arg1	parameters					1261:1270	the release parameters	1249:1270	the release parameters	1249:1270	Statistical analysis proved that anionic polymer type and chitosan: polymer ratio, as well as, their interactions, exhibited significant effects on the release parameters at p ≤ 0.05.
26942653	0	42	theme	implantable	27:37	arg1	sponge					51:56	implantable bioerodible sponge	27:56	implantable bioerodible sponge	27:56	Localized rosuvastatin via implantable bioerodible sponge and its potential role in augmenting bone healing and regeneration.
26942653	7	43	theme	microscopic	806:816	arg1	examination					818:828	microscopic examination	806:828	microscopic examination	806:828	RESULTS Visual and microscopic examination showed flexible homogenous porous structures with considerable bending ability.
26942653	6	44	theme	fractured	765:773	arg1	femora					779:784	fractured rat femora	765:784	fractured rat femora	765:784	The formula of choice was implanted in fractured rat femora.
26942653	8	45	theme	complex	926:932	arg1	formation					934:942	Polyelectrolyte complex formation	910:942	Polyelectrolyte complex formation	910:942	Polyelectrolyte complex formation was proved by DSC and FT-IR for all chitosan/anionic combinations except with xanthan gum where chitosan probably bound to the drug rather than xanthan gum.
26942653	15	46	theme	rosuvastatin	1815:1826	arg1	delivery					1828:1835	local rosuvastatin delivery	1809:1835	local rosuvastatin delivery	1809:1835	CONCLUSION Biodegradable sponges for local rosuvastatin delivery proved significantly enhanced wound healing and regeneration properties to fractured bones.
26942653	11	47	theme	other	1465:1469	arg1	ratios					1471:1476	other ratios	1465:1476	other ratios	1465:1476	The release at these ratios followed Fiction diffusion while other ratios had anomalous diffusion.
26942653	10	48	theme	complexation	1322:1333	arg1	3:1					1347:1349	3:1	1347:1349	3:1	1347:1349	The optimum chitosan/anionic polymer complexation ratios were 3:1 for polycarbophil and 1:1 for Carbopol and alginate.
26942653	10	48	theme	complexation	1322:1333	arg1	ratios					1335:1340	The optimum chitosan/anionic polymer complexation ratios	1285:1340	The optimum chitosan/anionic polymer complexation ratios	1285:1340	The optimum chitosan/anionic polymer complexation ratios were 3:1 for polycarbophil and 1:1 for Carbopol and alginate.
26942653	9	49	theme	anionic	1134:1140	arg1	interactions					1202:1213	their interactions	1196:1213	their interactions	1196:1213	Statistical analysis proved that anionic polymer type and chitosan: polymer ratio, as well as, their interactions, exhibited significant effects on the release parameters at p ≤ 0.05.
26942653	9	49	theme	anionic	1134:1140	arg1	polymer					1142:1148	anionic polymer type and chitosan	1134:1166	anionic polymer type and chitosan	1134:1166	Statistical analysis proved that anionic polymer type and chitosan: polymer ratio, as well as, their interactions, exhibited significant effects on the release parameters at p ≤ 0.05.
26942653	7	50	theme	Visual	795:800	arg1	RESULTS					787:793	RESULTS	787:793	RESULTS Visual	787:800	RESULTS Visual and microscopic examination showed flexible homogenous porous structures with considerable bending ability.
26942653	3	51	theme	current	273:279	arg1	work					281:284	the current work	269:284	the current work	269:284	In the current work, an attempt was investigated to develop, evaluate various bioerodible composite sponges enclosing rosuvastatin and explore their potential in augmenting bone healing and regeneration.
26942653	4	52	theme	METHOD	470:475	arg1	formulae					503:510	METHOD Twelve lyophilized sponge formulae	470:510	METHOD Twelve lyophilized sponge formulae	470:510	METHOD Twelve lyophilized sponge formulae were prepared adapting a 41.31 full factorial design.
26942653	2	53	theme	efficacious	246:256	arg1	Rosuvastatin					186:197	Rosuvastatin	186:197	Rosuvastatin	186:197	Rosuvastatin is a synthetic, hydrophilic, potent and highly efficacious statin.
26942653	2	53	theme	efficacious	246:256	arg1	statin					258:263	a synthetic, hydrophilic, potent and highly efficacious statin	202:263	a synthetic, hydrophilic, potent and highly efficacious statin	202:263	Rosuvastatin is a synthetic, hydrophilic, potent and highly efficacious statin.
26942653	15	54	theme	wound	1867:1871	arg1	healing					1873:1879	wound healing	1867:1879	wound healing	1867:1879	CONCLUSION Biodegradable sponges for local rosuvastatin delivery proved significantly enhanced wound healing and regeneration properties to fractured bones.
26942653	11	55	theme	anomalous	1482:1490	arg1	diffusion					1492:1500	anomalous diffusion	1482:1500	anomalous diffusion	1482:1500	The release at these ratios followed Fiction diffusion while other ratios had anomalous diffusion.
26942653	0	56	theme	bioerodible	39:49	arg1	sponge					51:56	implantable bioerodible sponge	27:56	implantable bioerodible sponge	27:56	Localized rosuvastatin via implantable bioerodible sponge and its potential role in augmenting bone healing and regeneration.
26942653	3	57	theme	bone	439:442	arg1	healing					444:450	bone healing	439:450	bone healing	439:450	In the current work, an attempt was investigated to develop, evaluate various bioerodible composite sponges enclosing rosuvastatin and explore their potential in augmenting bone healing and regeneration.
26942653	4	58	theme	sponge	496:501	arg1	formulae					503:510	METHOD Twelve lyophilized sponge formulae	470:510	METHOD Twelve lyophilized sponge formulae	470:510	METHOD Twelve lyophilized sponge formulae were prepared adapting a 41.31 full factorial design.
26942653	11	59	theme	Fiction	1441:1447	arg1	diffusion					1449:1457	Fiction diffusion	1441:1457	Fiction diffusion	1441:1457	The release at these ratios followed Fiction diffusion while other ratios had anomalous diffusion.
26942653	10	60	theme	polymer	1314:1320	arg1	3:1					1347:1349	3:1	1347:1349	3:1	1347:1349	The optimum chitosan/anionic polymer complexation ratios were 3:1 for polycarbophil and 1:1 for Carbopol and alginate.
26942653	10	60	theme	polymer	1314:1320	arg1	ratios					1335:1340	The optimum chitosan/anionic polymer complexation ratios	1285:1340	The optimum chitosan/anionic polymer complexation ratios	1285:1340	The optimum chitosan/anionic polymer complexation ratios were 3:1 for polycarbophil and 1:1 for Carbopol and alginate.
26942653	0	61	theme	potential	66:74	arg1	role					76:79	its potential role	62:79	its potential role in augmenting bone healing and regeneration	62:123	Localized rosuvastatin via implantable bioerodible sponge and its potential role in augmenting bone healing and regeneration.
26942653	9	62	from	effects	1238:1244	arg1	parameters					1261:1270	the release parameters	1249:1270	the release parameters	1249:1270	Statistical analysis proved that anionic polymer type and chitosan: polymer ratio, as well as, their interactions, exhibited significant effects on the release parameters at p ≤ 0.05.
26942653	12	63	theme	Imwitor®	1503:1510	arg1	K100M					1526:1530	HPMC K100M	1521:1530	HPMC K100M	1521:1530	Imwitor® 900K and HPMC K100M were added as release retarardants for further release optimization.
26942653	12	63	theme	Imwitor®	1503:1510	arg1	retarardants					1554:1565	release retarardants	1546:1565	release retarardants for further release optimization	1546:1598	Imwitor® 900K and HPMC K100M were added as release retarardants for further release optimization.
26942653	12	63	theme	Imwitor®	1503:1510	arg1	900K					1512:1515	Imwitor® 900K	1503:1515	Imwitor® 900K	1503:1515	Imwitor® 900K and HPMC K100M were added as release retarardants for further release optimization.
26942653	12	64	theme	HPMC	1521:1524	arg1	K100M					1526:1530	HPMC K100M	1521:1530	HPMC K100M	1521:1530	Imwitor® 900K and HPMC K100M were added as release retarardants for further release optimization.
26942653	12	64	theme	HPMC	1521:1524	arg1	900K					1512:1515	Imwitor® 900K	1503:1515	Imwitor® 900K	1503:1515	Imwitor® 900K and HPMC K100M were added as release retarardants for further release optimization.
26942653	12	64	theme	HPMC	1521:1524	arg1	retarardants					1554:1565	release retarardants	1546:1565	release retarardants for further release optimization	1546:1598	Imwitor® 900K and HPMC K100M were added as release retarardants for further release optimization.
26942653	2	65	theme	potent	228:233	arg1	Rosuvastatin					186:197	Rosuvastatin	186:197	Rosuvastatin	186:197	Rosuvastatin is a synthetic, hydrophilic, potent and highly efficacious statin.
26942653	2	65	theme	potent	228:233	arg1	statin					258:263	a synthetic, hydrophilic, potent and highly efficacious statin	202:263	a synthetic, hydrophilic, potent and highly efficacious statin	202:263	Rosuvastatin is a synthetic, hydrophilic, potent and highly efficacious statin.
26942653	9	66	theme	polymer	1169:1175	arg1	ratio					1177:1181	polymer ratio	1169:1181	polymer ratio	1169:1181	Statistical analysis proved that anionic polymer type and chitosan: polymer ratio, as well as, their interactions, exhibited significant effects on the release parameters at p ≤ 0.05.
26942653	1	67	theme	OBJECTIVE	126:134	arg1	Statins					136:142	OBJECTIVE Statins	126:142	OBJECTIVE Statins	126:142	OBJECTIVE Statins proved potential bone healing properties.
26942653	2	68	theme	hydrophilic	215:225	arg1	Rosuvastatin					186:197	Rosuvastatin	186:197	Rosuvastatin	186:197	Rosuvastatin is a synthetic, hydrophilic, potent and highly efficacious statin.
26942653	2	68	theme	hydrophilic	215:225	arg1	statin					258:263	a synthetic, hydrophilic, potent and highly efficacious statin	202:263	a synthetic, hydrophilic, potent and highly efficacious statin	202:263	Rosuvastatin is a synthetic, hydrophilic, potent and highly efficacious statin.
26942653	14	69	theme	treated	1731:1737	arg1	femora					1739:1744	treated femora	1731:1744	treated femora	1731:1744	Histopathological examination revealed advanced stages of healing in treated femora compared to control ones.
26942653	6	70	theme	choice	741:746	arg1	formula					730:736	The formula	726:736	The formula of choice	726:746	The formula of choice was implanted in fractured rat femora.
26942653	7	71	dep	flexible	837:844	arg1	homogenous					846:855	homogenous	846:855	homogenous	846:855	RESULTS Visual and microscopic examination showed flexible homogenous porous structures with considerable bending ability.
26942653	15	72	theme	regeneration	1885:1896	arg1	properties					1898:1907	significantly enhanced wound healing and regeneration properties	1844:1907	significantly enhanced wound healing and regeneration properties to fractured bones	1844:1926	CONCLUSION Biodegradable sponges for local rosuvastatin delivery proved significantly enhanced wound healing and regeneration properties to fractured bones.
26942653	14	73	theme	Histopathological	1662:1678	arg1	examination					1680:1690	Histopathological examination	1662:1690	Histopathological examination	1662:1690	Histopathological examination revealed advanced stages of healing in treated femora compared to control ones.
26942653	5	74	dep	investigated	629:640	arg1	ratios					702:707	anionic polymer ratios	686:707	anionic polymer ratios (1:3, 1:1, 3:1)	686:723	Xanthan gum, polycarbophil, Carbopol® and sodium alginate were investigated as anionic polymers, each at three chitosan:anionic polymer ratios (1:3, 1:1, 3:1).
26942653	8	75	theme	Polyelectrolyte	910:924	arg1	formation					934:942	Polyelectrolyte complex formation	910:942	Polyelectrolyte complex formation	910:942	Polyelectrolyte complex formation was proved by DSC and FT-IR for all chitosan/anionic combinations except with xanthan gum where chitosan probably bound to the drug rather than xanthan gum.
26942653	14	76	theme	control	1758:1764	arg1	ones					1766:1769	control ones	1758:1769	control ones	1758:1769	Histopathological examination revealed advanced stages of healing in treated femora compared to control ones.
26942653	2	77	theme	synthetic	204:212	arg1	Rosuvastatin					186:197	Rosuvastatin	186:197	Rosuvastatin	186:197	Rosuvastatin is a synthetic, hydrophilic, potent and highly efficacious statin.
26942653	2	77	theme	synthetic	204:212	arg1	statin					258:263	a synthetic, hydrophilic, potent and highly efficacious statin	202:263	a synthetic, hydrophilic, potent and highly efficacious statin	202:263	Rosuvastatin is a synthetic, hydrophilic, potent and highly efficacious statin.
26942653	1	78	theme	potential	151:159	arg1	properties					174:183	potential bone healing properties	151:183	potential bone healing properties	151:183	OBJECTIVE Statins proved potential bone healing properties.
26942653	3	79	theme	various	336:342	arg1	sponges					366:372	various bioerodible composite sponges	336:372	various bioerodible composite sponges enclosing rosuvastatin	336:395	In the current work, an attempt was investigated to develop, evaluate various bioerodible composite sponges enclosing rosuvastatin and explore their potential in augmenting bone healing and regeneration.
26942653	4	80	theme	41.31	537:541	arg1	design					558:563	a 41.31 full factorial design	535:563	a 41.31 full factorial design	535:563	METHOD Twelve lyophilized sponge formulae were prepared adapting a 41.31 full factorial design.
26942653	5	81	theme	Xanthan	566:572	arg1	alginate					615:622	sodium alginate	608:622	sodium alginate	608:622	Xanthan gum, polycarbophil, Carbopol® and sodium alginate were investigated as anionic polymers, each at three chitosan:anionic polymer ratios (1:3, 1:1, 3:1).
26942653	5	81	theme	Xanthan	566:572	arg1	polycarbophil					579:591	polycarbophil	579:591	polycarbophil	579:591	Xanthan gum, polycarbophil, Carbopol® and sodium alginate were investigated as anionic polymers, each at three chitosan:anionic polymer ratios (1:3, 1:1, 3:1).
26942653	5	81	theme	Xanthan	566:572	arg1	gum					574:576	Xanthan gum	566:576	Xanthan gum	566:576	Xanthan gum, polycarbophil, Carbopol® and sodium alginate were investigated as anionic polymers, each at three chitosan:anionic polymer ratios (1:3, 1:1, 3:1).
26942653	5	81	theme	Xanthan	566:572	arg1	Carbopol®					594:602	Carbopol®	594:602	Carbopol®	594:602	Xanthan gum, polycarbophil, Carbopol® and sodium alginate were investigated as anionic polymers, each at three chitosan:anionic polymer ratios (1:3, 1:1, 3:1).
26942653	5	81	theme	Xanthan	566:572	arg1	polymers					653:660	anionic polymers	645:660	anionic polymers	645:660	Xanthan gum, polycarbophil, Carbopol® and sodium alginate were investigated as anionic polymers, each at three chitosan:anionic polymer ratios (1:3, 1:1, 3:1).
26942653	1	82	theme	bone	161:164	arg1	properties					174:183	potential bone healing properties	151:183	potential bone healing properties	151:183	OBJECTIVE Statins proved potential bone healing properties.
26942653	10	83	theme	optimum	1289:1295	arg1	3:1					1347:1349	3:1	1347:1349	3:1	1347:1349	The optimum chitosan/anionic polymer complexation ratios were 3:1 for polycarbophil and 1:1 for Carbopol and alginate.
26942653	10	83	theme	optimum	1289:1295	arg1	ratios					1335:1340	The optimum chitosan/anionic polymer complexation ratios	1285:1340	The optimum chitosan/anionic polymer complexation ratios	1285:1340	The optimum chitosan/anionic polymer complexation ratios were 3:1 for polycarbophil and 1:1 for Carbopol and alginate.
26225911	1	0	theme	cartilage	366:374	arg1	repair					376:381	articular cartilage repair	356:381	articular cartilage repair	356:381	Chitosan/nano-hydroxyapatite (HA) composites were first prepared and then used together with chitosan and silk fibroin (SF) to produce a type of four-layer porous scaffold that is potentially applicable for articular cartilage repair.
26225911	2	1	theme	reversed	616:623	arg1	trend					625:629	a mutually reversed trend	605:629	a mutually reversed trend	605:629	The bottom layer of the scaffold was built with the chitosan/HA composite and the other three layers of the scaffold were fabricated using chitosan/SF composites in which the content of the chitosan and SF was altered in a mutually reversed trend.
26225911	2	2	theme	bottom	388:393	arg1	layer					395:399	The bottom layer	384:399	The bottom layer of the scaffold	384:415	The bottom layer of the scaffold was built with the chitosan/HA composite and the other three layers of the scaffold were fabricated using chitosan/SF composites in which the content of the chitosan and SF was altered in a mutually reversed trend.
26225911	4	3	theme	gradient	864:871	arg1	technique					884:892	a temperature gradient processing technique	850:892	a temperature gradient processing technique	850:892	Interconnected porous microstructures throughout the scaffolds were constructed using a temperature gradient processing technique, and the resultant scaffolds were endowed with graded pore-sizes and porosities as well as porous interface zones between contiguous layers without visual clefts.
26225911	4	4	with	endowed	928:934	arg1	porosities					963:972	porosities	963:972	porosities	963:972	Interconnected porous microstructures throughout the scaffolds were constructed using a temperature gradient processing technique, and the resultant scaffolds were endowed with graded pore-sizes and porosities as well as porous interface zones between contiguous layers without visual clefts.
26225911	4	4	with	endowed	928:934	arg1	pore-sizes					948:957	pore-sizes	948:957	pore-sizes	948:957	Interconnected porous microstructures throughout the scaffolds were constructed using a temperature gradient processing technique, and the resultant scaffolds were endowed with graded pore-sizes and porosities as well as porous interface zones between contiguous layers without visual clefts.
26225911	4	4	with	endowed	928:934	arg1	zones					1002:1006	porous interface zones	985:1006	graded pore-sizes and porosities as well as porous interface zones between contiguous layers without visual clefts	941:1054	Interconnected porous microstructures throughout the scaffolds were constructed using a temperature gradient processing technique, and the resultant scaffolds were endowed with graded pore-sizes and porosities as well as porous interface zones between contiguous layers without visual clefts.
26225911	4	5	theme	visual	1042:1047	arg1	clefts					1049:1054	visual clefts	1042:1054	visual clefts	1042:1054	Interconnected porous microstructures throughout the scaffolds were constructed using a temperature gradient processing technique, and the resultant scaffolds were endowed with graded pore-sizes and porosities as well as porous interface zones between contiguous layers without visual clefts.
26225911	3	6	theme	enhanced	732:739	arg1	properties					752:761	enhanced mechanical properties	732:761	enhanced mechanical properties	732:761	The so-produced chitosan/SF/HA scaffolds were further crosslinked using tripolyphosphate to achieve enhanced mechanical properties.
26225911	5	7	theme	mechanical	1207:1216	arg1	properties					1218:1227	the compressive mechanical properties	1191:1227	the compressive mechanical properties of an articular cartilage matrix	1191:1260	The compressive modulus and stress at 10% strain of the scaffolds in wet state showed a gradient-changed trend which partially mimics the compressive mechanical properties of an articular cartilage matrix.
26225911	6	8	theme	Cell	1263:1266	arg1	culture					1268:1274	Cell culture	1263:1274	Cell culture on some chitosan/SF/HA scaffolds for a period of time of up to 14 d	1263:1342	Cell culture on some chitosan/SF/HA scaffolds for a period of time of up to 14 d showed that the scaffolds were able to well support the growth and infiltration of cells, suggesting that the presently developed chitosan/SF/HA scaffolds have promising potential for articular cartilage repair.
26225911	0	9	theme	mechanical	126:135	arg1	properties					137:146	mechanical properties	126:146	mechanical properties	126:146	Fabrication and characterization of layered chitosan/silk fibroin/nano-hydroxyapatite scaffolds with designed composition and mechanical properties.
26225911	5	10	theme	compressive	1061:1071	arg1	modulus					1073:1079	The compressive modulus	1057:1079	The compressive modulus	1057:1079	The compressive modulus and stress at 10% strain of the scaffolds in wet state showed a gradient-changed trend which partially mimics the compressive mechanical properties of an articular cartilage matrix.
26225911	6	11	from	culture	1268:1274	arg1	scaffolds					1299:1307	some chitosan/SF/HA scaffolds	1279:1307	some chitosan/SF/HA scaffolds	1279:1307	Cell culture on some chitosan/SF/HA scaffolds for a period of time of up to 14 d showed that the scaffolds were able to well support the growth and infiltration of cells, suggesting that the presently developed chitosan/SF/HA scaffolds have promising potential for articular cartilage repair.
26225911	2	12	dep	chitosan	574:581	arg1	the					570:572	the	570:572	the	570:572	The bottom layer of the scaffold was built with the chitosan/HA composite and the other three layers of the scaffold were fabricated using chitosan/SF composites in which the content of the chitosan and SF was altered in a mutually reversed trend.
26225911	4	13	theme	temperature	852:862	arg1	technique					884:892	a temperature gradient processing technique	850:892	a temperature gradient processing technique	850:892	Interconnected porous microstructures throughout the scaffolds were constructed using a temperature gradient processing technique, and the resultant scaffolds were endowed with graded pore-sizes and porosities as well as porous interface zones between contiguous layers without visual clefts.
26225911	6	14	theme	chitosan/SF/HA	1474:1487	arg1	scaffolds					1489:1497	the presently developed chitosan/SF/HA scaffolds	1450:1497	the presently developed chitosan/SF/HA scaffolds	1450:1497	Cell culture on some chitosan/SF/HA scaffolds for a period of time of up to 14 d showed that the scaffolds were able to well support the growth and infiltration of cells, suggesting that the presently developed chitosan/SF/HA scaffolds have promising potential for articular cartilage repair.
26225911	2	15	theme	scaffold	492:499	arg1	layers					478:483	the other three layers	462:483	the other three layers of the scaffold	462:499	The bottom layer of the scaffold was built with the chitosan/HA composite and the other three layers of the scaffold were fabricated using chitosan/SF composites in which the content of the chitosan and SF was altered in a mutually reversed trend.
26225911	6	16	theme	articular	1528:1536	arg1	repair					1548:1553	articular cartilage repair	1528:1553	articular cartilage repair	1528:1553	Cell culture on some chitosan/SF/HA scaffolds for a period of time of up to 14 d showed that the scaffolds were able to well support the growth and infiltration of cells, suggesting that the presently developed chitosan/SF/HA scaffolds have promising potential for articular cartilage repair.
26225911	6	17	theme	promising	1504:1512	arg1	potential					1514:1522	promising potential	1504:1522	promising potential for articular cartilage repair	1504:1553	Cell culture on some chitosan/SF/HA scaffolds for a period of time of up to 14 d showed that the scaffolds were able to well support the growth and infiltration of cells, suggesting that the presently developed chitosan/SF/HA scaffolds have promising potential for articular cartilage repair.
26225911	6	18	theme	cells	1427:1431	arg1	growth					1400:1405	growth	1400:1405	growth	1400:1405	Cell culture on some chitosan/SF/HA scaffolds for a period of time of up to 14 d showed that the scaffolds were able to well support the growth and infiltration of cells, suggesting that the presently developed chitosan/SF/HA scaffolds have promising potential for articular cartilage repair.
26225911	6	18	theme	cells	1427:1431	arg1	infiltration					1411:1422	infiltration	1411:1422	infiltration	1411:1422	Cell culture on some chitosan/SF/HA scaffolds for a period of time of up to 14 d showed that the scaffolds were able to well support the growth and infiltration of cells, suggesting that the presently developed chitosan/SF/HA scaffolds have promising potential for articular cartilage repair.
26225911	6	19	theme	cartilage	1538:1546	arg1	repair					1548:1553	articular cartilage repair	1528:1553	articular cartilage repair	1528:1553	Cell culture on some chitosan/SF/HA scaffolds for a period of time of up to 14 d showed that the scaffolds were able to well support the growth and infiltration of cells, suggesting that the presently developed chitosan/SF/HA scaffolds have promising potential for articular cartilage repair.
26225911	5	20	theme	compressive	1195:1205	arg1	properties					1218:1227	the compressive mechanical properties	1191:1227	the compressive mechanical properties of an articular cartilage matrix	1191:1260	The compressive modulus and stress at 10% strain of the scaffolds in wet state showed a gradient-changed trend which partially mimics the compressive mechanical properties of an articular cartilage matrix.
26225911	4	21	theme	graded	941:946	arg1	pore-sizes					948:957	pore-sizes	948:957	pore-sizes	948:957	Interconnected porous microstructures throughout the scaffolds were constructed using a temperature gradient processing technique, and the resultant scaffolds were endowed with graded pore-sizes and porosities as well as porous interface zones between contiguous layers without visual clefts.
26225911	0	22	theme	designed	101:108	arg1	composition					110:120	designed composition	101:120	designed composition	101:120	Fabrication and characterization of layered chitosan/silk fibroin/nano-hydroxyapatite scaffolds with designed composition and mechanical properties.
26225911	2	23	theme	scaffold	408:415	arg1	layer					395:399	The bottom layer	384:399	The bottom layer of the scaffold	384:415	The bottom layer of the scaffold was built with the chitosan/HA composite and the other three layers of the scaffold were fabricated using chitosan/SF composites in which the content of the chitosan and SF was altered in a mutually reversed trend.
26225911	2	24	theme	other	466:470	arg1	layers					478:483	the other three layers	462:483	the other three layers of the scaffold	462:499	The bottom layer of the scaffold was built with the chitosan/HA composite and the other three layers of the scaffold were fabricated using chitosan/SF composites in which the content of the chitosan and SF was altered in a mutually reversed trend.
26225911	1	25	theme	four-layer	294:303	arg1	scaffold					312:319	four-layer porous scaffold	294:319	four-layer porous scaffold	294:319	Chitosan/nano-hydroxyapatite (HA) composites were first prepared and then used together with chitosan and silk fibroin (SF) to produce a type of four-layer porous scaffold that is potentially applicable for articular cartilage repair.
26225911	6	26	theme	up	1333:1334	arg1	time					1325:1328	time	1325:1328	time of up to 14 d	1325:1342	Cell culture on some chitosan/SF/HA scaffolds for a period of time of up to 14 d showed that the scaffolds were able to well support the growth and infiltration of cells, suggesting that the presently developed chitosan/SF/HA scaffolds have promising potential for articular cartilage repair.
26225911	1	27	theme	porous	305:310	arg1	scaffold					312:319	four-layer porous scaffold	294:319	four-layer porous scaffold	294:319	Chitosan/nano-hydroxyapatite (HA) composites were first prepared and then used together with chitosan and silk fibroin (SF) to produce a type of four-layer porous scaffold that is potentially applicable for articular cartilage repair.
26225911	2	28	theme	chitosan	574:581	arg1	content					559:565	the content	555:565	the content of the chitosan and SF	555:588	The bottom layer of the scaffold was built with the chitosan/HA composite and the other three layers of the scaffold were fabricated using chitosan/SF composites in which the content of the chitosan and SF was altered in a mutually reversed trend.
26225911	5	29	theme	scaffolds	1113:1121	arg1	strain					1099:1104	10% strain	1095:1104	10% strain of the scaffolds in wet state	1095:1134	The compressive modulus and stress at 10% strain of the scaffolds in wet state showed a gradient-changed trend which partially mimics the compressive mechanical properties of an articular cartilage matrix.
26225911	5	30	from	scaffolds	1113:1121	arg1	state					1130:1134	wet state	1126:1134	wet state	1126:1134	The compressive modulus and stress at 10% strain of the scaffolds in wet state showed a gradient-changed trend which partially mimics the compressive mechanical properties of an articular cartilage matrix.
26225911	6	31	theme	developed	1464:1472	arg1	scaffolds					1489:1497	the presently developed chitosan/SF/HA scaffolds	1450:1497	the presently developed chitosan/SF/HA scaffolds	1450:1497	Cell culture on some chitosan/SF/HA scaffolds for a period of time of up to 14 d showed that the scaffolds were able to well support the growth and infiltration of cells, suggesting that the presently developed chitosan/SF/HA scaffolds have promising potential for articular cartilage repair.
26225911	1	32	theme	scaffold	312:319	arg1	applicable					341:350	applicable	341:350	applicable	341:350	Chitosan/nano-hydroxyapatite (HA) composites were first prepared and then used together with chitosan and silk fibroin (SF) to produce a type of four-layer porous scaffold that is potentially applicable for articular cartilage repair.
26225911	1	32	theme	scaffold	312:319	arg1	type					286:289	a type	284:289	a type of four-layer porous scaffold that is potentially applicable for articular cartilage repair	284:381	Chitosan/nano-hydroxyapatite (HA) composites were first prepared and then used together with chitosan and silk fibroin (SF) to produce a type of four-layer porous scaffold that is potentially applicable for articular cartilage repair.
26225911	1	33	theme	Chitosan/nano-hydroxyapatite	149:176	arg1	composites					183:192	Chitosan/nano-hydroxyapatite (HA) composites	149:192	Chitosan/nano-hydroxyapatite (HA) composites	149:192	Chitosan/nano-hydroxyapatite (HA) composites were first prepared and then used together with chitosan and silk fibroin (SF) to produce a type of four-layer porous scaffold that is potentially applicable for articular cartilage repair.
26225911	5	34	from	strain	1099:1104	arg1	state					1130:1134	wet state	1126:1134	wet state	1126:1134	The compressive modulus and stress at 10% strain of the scaffolds in wet state showed a gradient-changed trend which partially mimics the compressive mechanical properties of an articular cartilage matrix.
26225911	5	34	from	strain	1099:1104	arg1	stress					1085:1090	stress	1085:1090	stress	1085:1090	The compressive modulus and stress at 10% strain of the scaffolds in wet state showed a gradient-changed trend which partially mimics the compressive mechanical properties of an articular cartilage matrix.
26225911	5	34	from	strain	1099:1104	arg1	modulus					1073:1079	The compressive modulus	1057:1079	The compressive modulus	1057:1079	The compressive modulus and stress at 10% strain of the scaffolds in wet state showed a gradient-changed trend which partially mimics the compressive mechanical properties of an articular cartilage matrix.
26225911	2	35	theme	chitosan/HA	436:446	arg1	composite					448:456	the chitosan/HA composite	432:456	the chitosan/HA composite	432:456	The bottom layer of the scaffold was built with the chitosan/HA composite and the other three layers of the scaffold were fabricated using chitosan/SF composites in which the content of the chitosan and SF was altered in a mutually reversed trend.
26225911	0	36	theme	chitosan/silk	44:56	arg1	fibroin/nano-hydroxyapatite					58:84	layered chitosan/silk fibroin/nano-hydroxyapatite	36:84	layered chitosan/silk fibroin/nano-hydroxyapatite	36:84	Fabrication and characterization of layered chitosan/silk fibroin/nano-hydroxyapatite scaffolds with designed composition and mechanical properties.
26225911	5	37	theme	gradient-changed	1145:1160	arg1	trend					1162:1166	a gradient-changed trend	1143:1166	a gradient-changed trend which partially mimics the compressive mechanical properties of an articular cartilage matrix	1143:1260	The compressive modulus and stress at 10% strain of the scaffolds in wet state showed a gradient-changed trend which partially mimics the compressive mechanical properties of an articular cartilage matrix.
26225911	1	38	theme	silk	255:258	arg1	SF					269:270	SF	269:270	SF	269:270	Chitosan/nano-hydroxyapatite (HA) composites were first prepared and then used together with chitosan and silk fibroin (SF) to produce a type of four-layer porous scaffold that is potentially applicable for articular cartilage repair.
26225911	1	38	theme	silk	255:258	arg1	fibroin					260:266	silk fibroin	255:266	silk fibroin (SF)	255:271	Chitosan/nano-hydroxyapatite (HA) composites were first prepared and then used together with chitosan and silk fibroin (SF) to produce a type of four-layer porous scaffold that is potentially applicable for articular cartilage repair.
26225911	0	39	theme	layered	36:42	arg1	fibroin/nano-hydroxyapatite					58:84	layered chitosan/silk fibroin/nano-hydroxyapatite	36:84	layered chitosan/silk fibroin/nano-hydroxyapatite	36:84	Fabrication and characterization of layered chitosan/silk fibroin/nano-hydroxyapatite scaffolds with designed composition and mechanical properties.
26225911	5	40	theme	articular	1235:1243	arg1	matrix					1255:1260	an articular cartilage matrix	1232:1260	an articular cartilage matrix	1232:1260	The compressive modulus and stress at 10% strain of the scaffolds in wet state showed a gradient-changed trend which partially mimics the compressive mechanical properties of an articular cartilage matrix.
26225911	4	41	theme	contiguous	1016:1025	arg1	layers					1027:1032	contiguous layers	1016:1032	contiguous layers without visual clefts	1016:1054	Interconnected porous microstructures throughout the scaffolds were constructed using a temperature gradient processing technique, and the resultant scaffolds were endowed with graded pore-sizes and porosities as well as porous interface zones between contiguous layers without visual clefts.
26225911	3	42	theme	chitosan/SF/HA	648:661	arg1	scaffolds					663:671	The so-produced chitosan/SF/HA scaffolds	632:671	The so-produced chitosan/SF/HA scaffolds	632:671	The so-produced chitosan/SF/HA scaffolds were further crosslinked using tripolyphosphate to achieve enhanced mechanical properties.
26225911	6	43	theme	time	1325:1328	arg1	period					1315:1320	a period	1313:1320	a period of time of up to 14 d	1313:1342	Cell culture on some chitosan/SF/HA scaffolds for a period of time of up to 14 d showed that the scaffolds were able to well support the growth and infiltration of cells, suggesting that the presently developed chitosan/SF/HA scaffolds have promising potential for articular cartilage repair.
26225911	5	44	theme	cartilage	1245:1253	arg1	matrix					1255:1260	an articular cartilage matrix	1232:1260	an articular cartilage matrix	1232:1260	The compressive modulus and stress at 10% strain of the scaffolds in wet state showed a gradient-changed trend which partially mimics the compressive mechanical properties of an articular cartilage matrix.
26225911	5	45	from	state	1130:1134	arg1	strain					1099:1104	10% strain	1095:1104	10% strain of the scaffolds in wet state	1095:1134	The compressive modulus and stress at 10% strain of the scaffolds in wet state showed a gradient-changed trend which partially mimics the compressive mechanical properties of an articular cartilage matrix.
26225911	4	46	theme	interface	992:1000	arg1	zones					1002:1006	porous interface zones	985:1006	graded pore-sizes and porosities as well as porous interface zones between contiguous layers without visual clefts	941:1054	Interconnected porous microstructures throughout the scaffolds were constructed using a temperature gradient processing technique, and the resultant scaffolds were endowed with graded pore-sizes and porosities as well as porous interface zones between contiguous layers without visual clefts.
26225911	4	47	theme	Interconnected	764:777	arg1	microstructures					786:800	Interconnected porous microstructures	764:800	Interconnected porous microstructures throughout the scaffolds	764:825	Interconnected porous microstructures throughout the scaffolds were constructed using a temperature gradient processing technique, and the resultant scaffolds were endowed with graded pore-sizes and porosities as well as porous interface zones between contiguous layers without visual clefts.
26225911	0	48	theme	fibroin/nano-hydroxyapatite	58:84	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Fabrication and characterization of layered chitosan/silk fibroin/nano-hydroxyapatite scaffolds with designed composition and mechanical properties.
26225911	0	48	theme	fibroin/nano-hydroxyapatite	58:84	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication	0:10	Fabrication and characterization of layered chitosan/silk fibroin/nano-hydroxyapatite scaffolds with designed composition and mechanical properties.
26225911	5	49	theme	matrix	1255:1260	arg1	properties					1218:1227	the compressive mechanical properties	1191:1227	the compressive mechanical properties of an articular cartilage matrix	1191:1260	The compressive modulus and stress at 10% strain of the scaffolds in wet state showed a gradient-changed trend which partially mimics the compressive mechanical properties of an articular cartilage matrix.
26225911	3	50	theme	mechanical	741:750	arg1	properties					752:761	enhanced mechanical properties	732:761	enhanced mechanical properties	732:761	The so-produced chitosan/SF/HA scaffolds were further crosslinked using tripolyphosphate to achieve enhanced mechanical properties.
26225911	4	51	theme	resultant	903:911	arg1	scaffolds					913:921	the resultant scaffolds	899:921	the resultant scaffolds	899:921	Interconnected porous microstructures throughout the scaffolds were constructed using a temperature gradient processing technique, and the resultant scaffolds were endowed with graded pore-sizes and porosities as well as porous interface zones between contiguous layers without visual clefts.
26225911	5	52	theme	10	1095:1096	arg1	%					1097:1097	%	1097:1097	%	1097:1097	The compressive modulus and stress at 10% strain of the scaffolds in wet state showed a gradient-changed trend which partially mimics the compressive mechanical properties of an articular cartilage matrix.
26225911	6	53	theme	chitosan/SF/HA	1284:1297	arg1	scaffolds					1299:1307	some chitosan/SF/HA scaffolds	1279:1307	some chitosan/SF/HA scaffolds	1279:1307	Cell culture on some chitosan/SF/HA scaffolds for a period of time of up to 14 d showed that the scaffolds were able to well support the growth and infiltration of cells, suggesting that the presently developed chitosan/SF/HA scaffolds have promising potential for articular cartilage repair.
26225911	2	54	theme	SF	587:588	arg1	content					559:565	the content	555:565	the content of the chitosan and SF	555:588	The bottom layer of the scaffold was built with the chitosan/HA composite and the other three layers of the scaffold were fabricated using chitosan/SF composites in which the content of the chitosan and SF was altered in a mutually reversed trend.
26225911	5	55	theme	wet	1126:1128	arg1	state					1130:1134	wet state	1126:1134	wet state	1126:1134	The compressive modulus and stress at 10% strain of the scaffolds in wet state showed a gradient-changed trend which partially mimics the compressive mechanical properties of an articular cartilage matrix.
26225911	4	56	theme	processing	873:882	arg1	technique					884:892	a temperature gradient processing technique	850:892	a temperature gradient processing technique	850:892	Interconnected porous microstructures throughout the scaffolds were constructed using a temperature gradient processing technique, and the resultant scaffolds were endowed with graded pore-sizes and porosities as well as porous interface zones between contiguous layers without visual clefts.
26225911	4	57	theme	porous	985:990	arg1	zones					1002:1006	porous interface zones	985:1006	graded pore-sizes and porosities as well as porous interface zones between contiguous layers without visual clefts	941:1054	Interconnected porous microstructures throughout the scaffolds were constructed using a temperature gradient processing technique, and the resultant scaffolds were endowed with graded pore-sizes and porosities as well as porous interface zones between contiguous layers without visual clefts.
26225911	3	58	theme	so-produced	636:646	arg1	scaffolds					663:671	The so-produced chitosan/SF/HA scaffolds	632:671	The so-produced chitosan/SF/HA scaffolds	632:671	The so-produced chitosan/SF/HA scaffolds were further crosslinked using tripolyphosphate to achieve enhanced mechanical properties.
26225911	4	59	theme	porous	779:784	arg1	microstructures					786:800	Interconnected porous microstructures	764:800	Interconnected porous microstructures throughout the scaffolds	764:825	Interconnected porous microstructures throughout the scaffolds were constructed using a temperature gradient processing technique, and the resultant scaffolds were endowed with graded pore-sizes and porosities as well as porous interface zones between contiguous layers without visual clefts.
26225911	2	60	theme	chitosan/SF	523:533	arg1	composites					535:544	chitosan/SF composites	523:544	chitosan/SF composites in which the content of the chitosan and SF was altered in a mutually reversed trend	523:629	The bottom layer of the scaffold was built with the chitosan/HA composite and the other three layers of the scaffold were fabricated using chitosan/SF composites in which the content of the chitosan and SF was altered in a mutually reversed trend.
26225911	1	61	used	used	223:226	arg2	composites					183:192	Chitosan/nano-hydroxyapatite (HA) composites	149:192	Chitosan/nano-hydroxyapatite (HA) composites	149:192	Chitosan/nano-hydroxyapatite (HA) composites were first prepared and then used together with chitosan and silk fibroin (SF) to produce a type of four-layer porous scaffold that is potentially applicable for articular cartilage repair.
26225911	1	62	theme	articular	356:364	arg1	repair					376:381	articular cartilage repair	356:381	articular cartilage repair	356:381	Chitosan/nano-hydroxyapatite (HA) composites were first prepared and then used together with chitosan and silk fibroin (SF) to produce a type of four-layer porous scaffold that is potentially applicable for articular cartilage repair.
26225911	5	63	theme	%	1097:1097	arg1	strain					1099:1104	10% strain	1095:1104	10% strain of the scaffolds in wet state	1095:1134	The compressive modulus and stress at 10% strain of the scaffolds in wet state showed a gradient-changed trend which partially mimics the compressive mechanical properties of an articular cartilage matrix.
26225911	6	64	contain	have	1499:1502	arg1	scaffolds					1489:1497	the presently developed chitosan/SF/HA scaffolds	1450:1497	the presently developed chitosan/SF/HA scaffolds	1450:1497	Cell culture on some chitosan/SF/HA scaffolds for a period of time of up to 14 d showed that the scaffolds were able to well support the growth and infiltration of cells, suggesting that the presently developed chitosan/SF/HA scaffolds have promising potential for articular cartilage repair.
26225911	6	64	contain	have	1499:1502	arg2	potential					1514:1522	promising potential	1504:1522	promising potential for articular cartilage repair	1504:1553	Cell culture on some chitosan/SF/HA scaffolds for a period of time of up to 14 d showed that the scaffolds were able to well support the growth and infiltration of cells, suggesting that the presently developed chitosan/SF/HA scaffolds have promising potential for articular cartilage repair.
28115099	5	0	theme	films	744:748	arg1	content					719:725	moisture content	710:725	moisture content	710:725	The water vapor permeability and moisture content of the composite films were lower than those of gelatin.
28115099	5	0	theme	films	744:748	arg1	permeability					693:704	water vapor permeability	681:704	water vapor permeability	681:704	The water vapor permeability and moisture content of the composite films were lower than those of gelatin.
28115099	3	1	theme	barrier	470:476	arg1	properties					478:487	barrier properties	470:487	barrier properties	470:487	The drying kinetics, thermomechanical, physicochemical, and barrier properties of composite films were estimated and compared with bovine gelatin.
28115099	3	1	theme	barrier	470:476	arg1	kinetics					421:428	The drying kinetics	410:428	The drying kinetics	410:428	The drying kinetics, thermomechanical, physicochemical, and barrier properties of composite films were estimated and compared with bovine gelatin.
28115099	1	2	theme	gelatin	232:238	arg1	alternative					240:250	a gelatin alternative	230:250	a gelatin alternative in the pharmaceutical capsules processing	230:292	The aim of this study was development a composite film based on sago starch and κ-carrageenan to find a gelatin alternative in the pharmaceutical capsules processing.
28115099	6	3	theme	composite	863:871	arg1	films					873:877	the composite films	859:877	the composite films	859:877	The solubility of the composite films was higher than that of gelatin, and the composite films were more stable at higher relative humidity than were the gelatin films.
28115099	6	3	theme	composite	863:871	arg1	stable					889:894	stable	889:894	stable	889:894	The solubility of the composite films was higher than that of gelatin, and the composite films were more stable at higher relative humidity than were the gelatin films.
28115099	6	4	dep	films	946:950	arg1	than					924:927	than	924:927	than	924:927	The solubility of the composite films was higher than that of gelatin, and the composite films were more stable at higher relative humidity than were the gelatin films.
28115099	6	4	dep	films	946:950	arg1	were					929:932	were	929:932	were	929:932	The solubility of the composite films was higher than that of gelatin, and the composite films were more stable at higher relative humidity than were the gelatin films.
28115099	6	5	theme	gelatin	938:944	arg1	films					946:950	the gelatin films	934:950	the gelatin films	934:950	The solubility of the composite films was higher than that of gelatin, and the composite films were more stable at higher relative humidity than were the gelatin films.
28115099	5	6	theme	moisture	710:717	arg1	content					719:725	moisture content	710:725	moisture content	710:725	The water vapor permeability and moisture content of the composite films were lower than those of gelatin.
28115099	7	7	theme	capsules	1134:1141	arg1	processes					1143:1151	pharmaceutical capsules processes	1119:1151	pharmaceutical capsules processes	1119:1151	These results show that dually modified sago starch in combination with κ-carrageenan has properties similar to those of gelatin, thus proposed system can be used in pharmaceutical capsules processes.
28115099	7	8	theme	similar	1054:1060	arg1	properties					1043:1052	properties	1043:1052	properties similar to those of gelatin	1043:1080	These results show that dually modified sago starch in combination with κ-carrageenan has properties similar to those of gelatin, thus proposed system can be used in pharmaceutical capsules processes.
28115099	7	9	with	combination	1008:1018	arg1	κ-carrageenan					1025:1037	κ-carrageenan	1025:1037	κ-carrageenan	1025:1037	These results show that dually modified sago starch in combination with κ-carrageenan has properties similar to those of gelatin, thus proposed system can be used in pharmaceutical capsules processes.
28115099	3	10	theme	films	502:506	arg1	kinetics					421:428	The drying kinetics	410:428	The drying kinetics	410:428	The drying kinetics, thermomechanical, physicochemical, and barrier properties of composite films were estimated and compared with bovine gelatin.
28115099	3	10	theme	films	502:506	arg1	properties					478:487	barrier properties	470:487	barrier properties	470:487	The drying kinetics, thermomechanical, physicochemical, and barrier properties of composite films were estimated and compared with bovine gelatin.
28115099	3	10	theme	films	502:506	arg1	physicochemical					449:463	physicochemical	449:463	physicochemical	449:463	The drying kinetics, thermomechanical, physicochemical, and barrier properties of composite films were estimated and compared with bovine gelatin.
28115099	3	10	theme	films	502:506	arg1	thermomechanical					431:446	thermomechanical	431:446	thermomechanical	431:446	The drying kinetics, thermomechanical, physicochemical, and barrier properties of composite films were estimated and compared with bovine gelatin.
28115099	0	11	theme	pharmaceutical	103:116	arg1	capsules					118:125	pharmaceutical capsules	103:125	pharmaceutical capsules	103:125	Functional properties of dually modified sago starch/κ-carrageenan films: An alternative to gelatin in pharmaceutical capsules.
28115099	4	12	theme	drying	575:580	arg1	kinetics					582:589	drying kinetics	575:589	drying kinetics	575:589	Results show that drying kinetics and mechanical properties of the composite films were comparable to those of gelatin.
28115099	1	13	theme	composite	168:176	arg1	film					178:181	a composite film	166:181	a composite film based on sago starch and κ-carrageenan	166:220	The aim of this study was development a composite film based on sago starch and κ-carrageenan to find a gelatin alternative in the pharmaceutical capsules processing.
28115099	3	14	theme	bovine	541:546	arg1	gelatin					548:554	bovine gelatin	541:554	bovine gelatin	541:554	The drying kinetics, thermomechanical, physicochemical, and barrier properties of composite films were estimated and compared with bovine gelatin.
28115099	4	15	theme	composite	624:632	arg1	films					634:638	the composite films	620:638	the composite films	620:638	Results show that drying kinetics and mechanical properties of the composite films were comparable to those of gelatin.
28115099	0	16	theme	Functional	0:9	arg1	properties					11:20	Functional properties	0:20	Functional properties of dually modified sago starch/κ-carrageenan films: An alternative to gelatin in pharmaceutical capsules.	0:126	Functional properties of dually modified sago starch/κ-carrageenan films: An alternative to gelatin in pharmaceutical capsules.
28115099	1	17	theme	pharmaceutical	259:272	arg1	processing					283:292	the pharmaceutical capsules processing	255:292	the pharmaceutical capsules processing	255:292	The aim of this study was development a composite film based on sago starch and κ-carrageenan to find a gelatin alternative in the pharmaceutical capsules processing.
28115099	1	18	theme	capsules	274:281	arg1	processing					283:292	the pharmaceutical capsules processing	255:292	the pharmaceutical capsules processing	255:292	The aim of this study was development a composite film based on sago starch and κ-carrageenan to find a gelatin alternative in the pharmaceutical capsules processing.
28115099	6	19	from	humidity	915:922	arg1	films					873:877	the composite films	859:877	the composite films	859:877	The solubility of the composite films was higher than that of gelatin, and the composite films were more stable at higher relative humidity than were the gelatin films.
28115099	6	19	from	humidity	915:922	arg1	stable					889:894	stable	889:894	stable	889:894	The solubility of the composite films was higher than that of gelatin, and the composite films were more stable at higher relative humidity than were the gelatin films.
28115099	5	20	theme	water	681:685	arg1	permeability					693:704	water vapor permeability	681:704	water vapor permeability	681:704	The water vapor permeability and moisture content of the composite films were lower than those of gelatin.
28115099	2	21	theme	Hydrolyzed-Hydroxypropylated	295:322	arg1	starch					347:352	Hydrolyzed-Hydroxypropylated (dually modified) sago starch	295:352	Hydrolyzed-Hydroxypropylated (dually modified) sago starch	295:352	Hydrolyzed-Hydroxypropylated (dually modified) sago starch was mixed with κ-carrageenan (0.25, 0.5, 0.75, and 1%).
28115099	1	22	theme	sago	192:195	arg1	starch					197:202	sago starch	192:202	sago starch	192:202	The aim of this study was development a composite film based on sago starch and κ-carrageenan to find a gelatin alternative in the pharmaceutical capsules processing.
28115099	0	23	theme	sago	41:44	arg1	films					67:71	dually modified sago starch/κ-carrageenan films	25:71	dually modified sago starch/κ-carrageenan films	25:71	Functional properties of dually modified sago starch/κ-carrageenan films: An alternative to gelatin in pharmaceutical capsules.
28115099	6	24	theme	relative	906:913	arg1	humidity					915:922	higher relative humidity	899:922	higher relative humidity	899:922	The solubility of the composite films was higher than that of gelatin, and the composite films were more stable at higher relative humidity than were the gelatin films.
28115099	5	25	theme	vapor	687:691	arg1	permeability					693:704	water vapor permeability	681:704	water vapor permeability	681:704	The water vapor permeability and moisture content of the composite films were lower than those of gelatin.
28115099	0	26	from	alternative	77:87	arg1	capsules					118:125	pharmaceutical capsules	103:125	pharmaceutical capsules	103:125	Functional properties of dually modified sago starch/κ-carrageenan films: An alternative to gelatin in pharmaceutical capsules.
28115099	3	27	theme	drying	414:419	arg1	kinetics					421:428	The drying kinetics	410:428	The drying kinetics	410:428	The drying kinetics, thermomechanical, physicochemical, and barrier properties of composite films were estimated and compared with bovine gelatin.
28115099	3	27	theme	drying	414:419	arg1	properties					478:487	barrier properties	470:487	barrier properties	470:487	The drying kinetics, thermomechanical, physicochemical, and barrier properties of composite films were estimated and compared with bovine gelatin.
28115099	3	27	theme	drying	414:419	arg1	physicochemical					449:463	physicochemical	449:463	physicochemical	449:463	The drying kinetics, thermomechanical, physicochemical, and barrier properties of composite films were estimated and compared with bovine gelatin.
28115099	3	27	theme	drying	414:419	arg1	thermomechanical					431:446	thermomechanical	431:446	thermomechanical	431:446	The drying kinetics, thermomechanical, physicochemical, and barrier properties of composite films were estimated and compared with bovine gelatin.
28115099	0	28	theme	modified	32:39	arg1	films					67:71	dually modified sago starch/κ-carrageenan films	25:71	dually modified sago starch/κ-carrageenan films	25:71	Functional properties of dually modified sago starch/κ-carrageenan films: An alternative to gelatin in pharmaceutical capsules.
28115099	6	29	theme	higher	899:904	arg1	humidity					915:922	higher relative humidity	899:922	higher relative humidity	899:922	The solubility of the composite films was higher than that of gelatin, and the composite films were more stable at higher relative humidity than were the gelatin films.
28115099	1	30	from	alternative	240:250	arg1	processing					283:292	the pharmaceutical capsules processing	255:292	the pharmaceutical capsules processing	255:292	The aim of this study was development a composite film based on sago starch and κ-carrageenan to find a gelatin alternative in the pharmaceutical capsules processing.
28115099	0	31	theme	films	67:71	arg1	properties					11:20	Functional properties	0:20	Functional properties of dually modified sago starch/κ-carrageenan films: An alternative to gelatin in pharmaceutical capsules.	0:126	Functional properties of dually modified sago starch/κ-carrageenan films: An alternative to gelatin in pharmaceutical capsules.
28115099	7	32	theme	sago	993:996	arg1	starch					998:1003	dually modified sago starch	977:1003	dually modified sago starch in combination with κ-carrageenan	977:1037	These results show that dually modified sago starch in combination with κ-carrageenan has properties similar to those of gelatin, thus proposed system can be used in pharmaceutical capsules processes.
28115099	5	33	dep	permeability	693:704	arg1	The					677:679	The	677:679	The	677:679	The water vapor permeability and moisture content of the composite films were lower than those of gelatin.
28115099	0	34	theme	starch/κ-carrageenan	46:65	arg1	films					67:71	dually modified sago starch/κ-carrageenan films	25:71	dually modified sago starch/κ-carrageenan films	25:71	Functional properties of dually modified sago starch/κ-carrageenan films: An alternative to gelatin in pharmaceutical capsules.
28115099	7	35	used	used	1111:1114	arg2	system					1097:1102	proposed system	1088:1102	proposed system	1088:1102	These results show that dually modified sago starch in combination with κ-carrageenan has properties similar to those of gelatin, thus proposed system can be used in pharmaceutical capsules processes.
28115099	6	36	theme	films	816:820	arg1	higher					826:831	higher	826:831	higher	826:831	The solubility of the composite films was higher than that of gelatin, and the composite films were more stable at higher relative humidity than were the gelatin films.
28115099	6	36	theme	films	816:820	arg1	solubility					788:797	The solubility	784:797	The solubility of the composite films	784:820	The solubility of the composite films was higher than that of gelatin, and the composite films were more stable at higher relative humidity than were the gelatin films.
28115099	2	37	dep	Hydrolyzed-Hydroxypropylated	295:322	arg1	modified					332:339	modified	332:339	modified	332:339	Hydrolyzed-Hydroxypropylated (dually modified) sago starch was mixed with κ-carrageenan (0.25, 0.5, 0.75, and 1%).
28115099	6	38	theme	composite	806:814	arg1	films					816:820	the composite films	802:820	the composite films	802:820	The solubility of the composite films was higher than that of gelatin, and the composite films were more stable at higher relative humidity than were the gelatin films.
28115099	7	39	theme	proposed	1088:1095	arg1	system					1097:1102	proposed system	1088:1102	proposed system	1088:1102	These results show that dually modified sago starch in combination with κ-carrageenan has properties similar to those of gelatin, thus proposed system can be used in pharmaceutical capsules processes.
28115099	2	40	dep	modified	332:339	arg1	dually					325:330	dually	325:330	dually	325:330	Hydrolyzed-Hydroxypropylated (dually modified) sago starch was mixed with κ-carrageenan (0.25, 0.5, 0.75, and 1%).
28115099	7	41	contain	has	1039:1041	arg1	starch					998:1003	dually modified sago starch	977:1003	dually modified sago starch in combination with κ-carrageenan	977:1037	These results show that dually modified sago starch in combination with κ-carrageenan has properties similar to those of gelatin, thus proposed system can be used in pharmaceutical capsules processes.
28115099	7	41	contain	has	1039:1041	arg2	properties					1043:1052	properties	1043:1052	properties similar to those of gelatin	1043:1080	These results show that dually modified sago starch in combination with κ-carrageenan has properties similar to those of gelatin, thus proposed system can be used in pharmaceutical capsules processes.
28115099	7	42	theme	modified	984:991	arg1	starch					998:1003	dually modified sago starch	977:1003	dually modified sago starch in combination with κ-carrageenan	977:1037	These results show that dually modified sago starch in combination with κ-carrageenan has properties similar to those of gelatin, thus proposed system can be used in pharmaceutical capsules processes.
28115099	3	43	theme	composite	492:500	arg1	films					502:506	composite films	492:506	composite films	492:506	The drying kinetics, thermomechanical, physicochemical, and barrier properties of composite films were estimated and compared with bovine gelatin.
28115099	2	44	theme	sago	342:345	arg1	starch					347:352	Hydrolyzed-Hydroxypropylated (dually modified) sago starch	295:352	Hydrolyzed-Hydroxypropylated (dually modified) sago starch	295:352	Hydrolyzed-Hydroxypropylated (dually modified) sago starch was mixed with κ-carrageenan (0.25, 0.5, 0.75, and 1%).
28115099	0	45	dep	properties	11:20	arg1	alternative					77:87	An alternative	74:87	Functional properties of dually modified sago starch/κ-carrageenan films: An alternative to gelatin in pharmaceutical capsules.	0:126	Functional properties of dually modified sago starch/κ-carrageenan films: An alternative to gelatin in pharmaceutical capsules.
28115099	4	46	theme	films	634:638	arg1	kinetics					582:589	drying kinetics	575:589	drying kinetics	575:589	Results show that drying kinetics and mechanical properties of the composite films were comparable to those of gelatin.
28115099	4	46	theme	films	634:638	arg1	properties					606:615	mechanical properties	595:615	mechanical properties of the composite films	595:638	Results show that drying kinetics and mechanical properties of the composite films were comparable to those of gelatin.
28115099	4	47	theme	mechanical	595:604	arg1	properties					606:615	mechanical properties	595:615	mechanical properties of the composite films	595:638	Results show that drying kinetics and mechanical properties of the composite films were comparable to those of gelatin.
28115099	5	48	theme	composite	734:742	arg1	films					744:748	the composite films	730:748	the composite films	730:748	The water vapor permeability and moisture content of the composite films were lower than those of gelatin.
28115099	1	49	theme	study	144:148	arg1	aim					132:134	The aim	128:134	The aim of this study	128:148	The aim of this study was development a composite film based on sago starch and κ-carrageenan to find a gelatin alternative in the pharmaceutical capsules processing.
28115099	1	49	theme	study	144:148	arg1	development					154:164	development	154:164	development a composite film based on sago starch and κ-carrageenan to find a gelatin alternative in the pharmaceutical capsules processing	154:292	The aim of this study was development a composite film based on sago starch and κ-carrageenan to find a gelatin alternative in the pharmaceutical capsules processing.
28115099	7	50	theme	pharmaceutical	1119:1132	arg1	processes					1143:1151	pharmaceutical capsules processes	1119:1151	pharmaceutical capsules processes	1119:1151	These results show that dually modified sago starch in combination with κ-carrageenan has properties similar to those of gelatin, thus proposed system can be used in pharmaceutical capsules processes.
28115099	7	51	from	starch	998:1003	arg1	combination					1008:1018	combination	1008:1018	combination with κ-carrageenan	1008:1037	These results show that dually modified sago starch in combination with κ-carrageenan has properties similar to those of gelatin, thus proposed system can be used in pharmaceutical capsules processes.
24328148	10	0	theme	individuals	1918:1928	arg1	clusters					1906:1913	simpler clusters	1898:1913	simpler clusters of individuals whose N-linked glycans share structural features	1898:1977	Our results emphasize that individual variation in protein glycosylation must be considered when assessing the value of a glycoproteomic marker, but also indicate that glycosylation diversity across human subjects can be reduced to simpler clusters of individuals whose N-linked glycans share structural features.
24328148	7	1	from	changes	1241:1247	arg1	abundance					1252:1260	abundance	1252:1260	abundance	1252:1260	Validation of these changes in abundance by Western blotting revealed increased REG protein glycoform diversity in cancer.
24328148	4	2	theme	glycome	703:709	arg1	characterization					670:685	MS(n)-based characterization	658:685	MS(n)-based characterization of the N-linked glycome of a small set of pancreatic ductal fluid obtained from normal, pancreatitis, intraductal papillary mucinous neoplasm (IPMN), and pancreatic adenocarcinoma patients	658:874	In order to assess this possibility for pancreatic cancer, we have performed an in-depth LC-MS/MS analysis of the proteome and MS(n)-based characterization of the N-linked glycome of a small set of pancreatic ductal fluid obtained from normal, pancreatitis, intraductal papillary mucinous neoplasm (IPMN), and pancreatic adenocarcinoma patients.
24328148	4	2	theme	glycome	703:709	arg1	analysis					629:636	an in-depth LC-MS/MS analysis	608:636	an in-depth LC-MS/MS analysis of the proteome	608:652	In order to assess this possibility for pancreatic cancer, we have performed an in-depth LC-MS/MS analysis of the proteome and MS(n)-based characterization of the N-linked glycome of a small set of pancreatic ductal fluid obtained from normal, pancreatitis, intraductal papillary mucinous neoplasm (IPMN), and pancreatic adenocarcinoma patients.
24328148	2	3	theme	early	349:353	arg1	detection					355:363	early detection	349:363	early detection	349:363	The high mortality associated with adenocarcinoma of the pancreatic epithelium justifies the broadest possible search for new biomarkers that can facilitate early detection or monitor treatment efficacy.
24328148	9	4	theme	samples	1657:1663	arg1	samples					1657:1663	samples	1657:1663	samples	1657:1663	Within each group, the profiles of less prevalent glycans were able to distinguish normal from cancer on this small set of samples.
24328148	9	4	theme	samples	1657:1663	arg1	set					1650:1652	this small set	1639:1652	this small set of samples	1639:1663	Within each group, the profiles of less prevalent glycans were able to distinguish normal from cancer on this small set of samples.
24328148	7	5	from	abundance	1252:1260	arg1	Validation					1221:1230	Validation	1221:1230	Validation of these changes in abundance by Western blotting	1221:1280	Validation of these changes in abundance by Western blotting revealed increased REG protein glycoform diversity in cancer.
24328148	10	6	theme	N-linked	1936:1943	arg1	glycans					1945:1951	N-linked glycans	1936:1951	N-linked glycans	1936:1951	Our results emphasize that individual variation in protein glycosylation must be considered when assessing the value of a glycoproteomic marker, but also indicate that glycosylation diversity across human subjects can be reduced to simpler clusters of individuals whose N-linked glycans share structural features.
24328148	8	7	theme	adenocarcinoma	1412:1425	arg1	samples					1450:1456	adenocarcinoma ductal fluid clustered samples	1412:1456	adenocarcinoma ductal fluid clustered samples	1412:1456	Characterization of the total N-linked glycome of normal, IPMN, and adenocarcinoma ductal fluid clustered samples into three discrete groups based on the prevalence of six dominant glycans.
24328148	6	8	theme	pancreatic	1203:1212	arg1	cells					1214:1218	normal pancreatic cells	1196:1218	normal pancreatic cells	1196:1218	These proteins are all directly or indirectly associated with the secretory pathway in normal pancreatic cells.
24328148	2	9	theme	epithelium	260:269	arg1	adenocarcinoma					227:240	adenocarcinoma	227:240	adenocarcinoma of the pancreatic epithelium	227:269	The high mortality associated with adenocarcinoma of the pancreatic epithelium justifies the broadest possible search for new biomarkers that can facilitate early detection or monitor treatment efficacy.
24328148	8	10	theme	fluid	1434:1438	arg1	samples					1450:1456	adenocarcinoma ductal fluid clustered samples	1412:1456	adenocarcinoma ductal fluid clustered samples	1412:1456	Characterization of the total N-linked glycome of normal, IPMN, and adenocarcinoma ductal fluid clustered samples into three discrete groups based on the prevalence of six dominant glycans.
24328148	4	11	theme	pancreatic	729:738	arg1	fluid					747:751	pancreatic ductal fluid	729:751	pancreatic ductal fluid obtained from normal, pancreatitis, intraductal papillary mucinous neoplasm (IPMN), and pancreatic adenocarcinoma patients	729:874	In order to assess this possibility for pancreatic cancer, we have performed an in-depth LC-MS/MS analysis of the proteome and MS(n)-based characterization of the N-linked glycome of a small set of pancreatic ductal fluid obtained from normal, pancreatitis, intraductal papillary mucinous neoplasm (IPMN), and pancreatic adenocarcinoma patients.
24328148	10	12	theme	protein	1717:1723	arg1	glycosylation					1725:1737	protein glycosylation	1717:1737	protein glycosylation	1717:1737	Our results emphasize that individual variation in protein glycosylation must be considered when assessing the value of a glycoproteomic marker, but also indicate that glycosylation diversity across human subjects can be reduced to simpler clusters of individuals whose N-linked glycans share structural features.
24328148	8	13	theme	samples	1450:1456	arg1	glycome					1383:1389	the total N-linked glycome	1364:1389	the total N-linked glycome of normal, IPMN, and adenocarcinoma ductal fluid clustered samples into three discrete groups	1364:1483	Characterization of the total N-linked glycome of normal, IPMN, and adenocarcinoma ductal fluid clustered samples into three discrete groups based on the prevalence of six dominant glycans.
24328148	6	14	theme	secretory	1175:1183	arg1	pathway					1185:1191	the secretory pathway	1171:1191	the secretory pathway in normal pancreatic cells	1171:1218	These proteins are all directly or indirectly associated with the secretory pathway in normal pancreatic cells.
24328148	4	15	theme	fluid	747:751	arg1	set					722:724	a small set	714:724	a small set of pancreatic ductal fluid obtained from normal, pancreatitis, intraductal papillary mucinous neoplasm (IPMN), and pancreatic adenocarcinoma patients	714:874	In order to assess this possibility for pancreatic cancer, we have performed an in-depth LC-MS/MS analysis of the proteome and MS(n)-based characterization of the N-linked glycome of a small set of pancreatic ductal fluid obtained from normal, pancreatitis, intraductal papillary mucinous neoplasm (IPMN), and pancreatic adenocarcinoma patients.
24328148	4	15	theme	fluid	747:751	arg1	fluid					747:751	pancreatic ductal fluid	729:751	pancreatic ductal fluid obtained from normal, pancreatitis, intraductal papillary mucinous neoplasm (IPMN), and pancreatic adenocarcinoma patients	729:874	In order to assess this possibility for pancreatic cancer, we have performed an in-depth LC-MS/MS analysis of the proteome and MS(n)-based characterization of the N-linked glycome of a small set of pancreatic ductal fluid obtained from normal, pancreatitis, intraductal papillary mucinous neoplasm (IPMN), and pancreatic adenocarcinoma patients.
24328148	6	16	from	pathway	1185:1191	arg1	cells					1214:1218	normal pancreatic cells	1196:1218	normal pancreatic cells	1196:1218	These proteins are all directly or indirectly associated with the secretory pathway in normal pancreatic cells.
24328148	2	17	theme	possible	294:301	arg1	search					303:308	the broadest possible search	281:308	the broadest possible search for new biomarkers that can facilitate early detection or monitor treatment efficacy	281:393	The high mortality associated with adenocarcinoma of the pancreatic epithelium justifies the broadest possible search for new biomarkers that can facilitate early detection or monitor treatment efficacy.
24328148	1	18	theme	pancreatic	147:156	arg1	cancer					158:163	pancreatic cancer	147:163	pancreatic cancer	147:163	Sensitive and specific biomarkers for pancreatic cancer are currently unavailable.
24328148	10	19	theme	glycoproteomic	1788:1801	arg1	marker					1803:1808	a glycoproteomic marker	1786:1808	a glycoproteomic marker	1786:1808	Our results emphasize that individual variation in protein glycosylation must be considered when assessing the value of a glycoproteomic marker, but also indicate that glycosylation diversity across human subjects can be reduced to simpler clusters of individuals whose N-linked glycans share structural features.
24328148	8	20	link	N-linked	1374:1381	arg1	glycome					1383:1389	the total N-linked glycome	1364:1389	the total N-linked glycome of normal, IPMN, and adenocarcinoma ductal fluid clustered samples into three discrete groups	1364:1483	Characterization of the total N-linked glycome of normal, IPMN, and adenocarcinoma ductal fluid clustered samples into three discrete groups based on the prevalence of six dominant glycans.
24328148	4	21	theme	in-depth	611:618	arg1	analysis					629:636	an in-depth LC-MS/MS analysis	608:636	an in-depth LC-MS/MS analysis of the proteome	608:652	In order to assess this possibility for pancreatic cancer, we have performed an in-depth LC-MS/MS analysis of the proteome and MS(n)-based characterization of the N-linked glycome of a small set of pancreatic ductal fluid obtained from normal, pancreatitis, intraductal papillary mucinous neoplasm (IPMN), and pancreatic adenocarcinoma patients.
24328148	4	22	theme	set	722:724	arg1	glycome					703:709	the N-linked glycome	690:709	the N-linked glycome of a small set of pancreatic ductal fluid obtained from normal, pancreatitis, intraductal papillary mucinous neoplasm (IPMN), and pancreatic adenocarcinoma patients	690:874	In order to assess this possibility for pancreatic cancer, we have performed an in-depth LC-MS/MS analysis of the proteome and MS(n)-based characterization of the N-linked glycome of a small set of pancreatic ductal fluid obtained from normal, pancreatitis, intraductal papillary mucinous neoplasm (IPMN), and pancreatic adenocarcinoma patients.
24328148	5	23	dep	increased	945:953	arg1	compared					978:985	compared	978:985	compared to normal (AMYP, PRSS1, GP2-1, CCDC132, REG1A, REG1B, and REG3A) and one protein that was consistently decreased (LIPR2)	978:1106	Our results identify a set of seven proteins that were consistently increased in cancer ductal fluid compared to normal (AMYP, PRSS1, GP2-1, CCDC132, REG1A, REG1B, and REG3A) and one protein that was consistently decreased (LIPR2).
24328148	7	24	from	diversity	1323:1331	arg1	cancer					1336:1341	cancer	1336:1341	cancer	1336:1341	Validation of these changes in abundance by Western blotting revealed increased REG protein glycoform diversity in cancer.
24328148	4	25	theme	ductal	740:745	arg1	fluid					747:751	pancreatic ductal fluid	729:751	pancreatic ductal fluid obtained from normal, pancreatitis, intraductal papillary mucinous neoplasm (IPMN), and pancreatic adenocarcinoma patients	729:874	In order to assess this possibility for pancreatic cancer, we have performed an in-depth LC-MS/MS analysis of the proteome and MS(n)-based characterization of the N-linked glycome of a small set of pancreatic ductal fluid obtained from normal, pancreatitis, intraductal papillary mucinous neoplasm (IPMN), and pancreatic adenocarcinoma patients.
24328148	4	26	theme	papillary	801:809	arg1	IPMN					830:833	IPMN	830:833	IPMN	830:833	In order to assess this possibility for pancreatic cancer, we have performed an in-depth LC-MS/MS analysis of the proteome and MS(n)-based characterization of the N-linked glycome of a small set of pancreatic ductal fluid obtained from normal, pancreatitis, intraductal papillary mucinous neoplasm (IPMN), and pancreatic adenocarcinoma patients.
24328148	4	26	theme	papillary	801:809	arg1	neoplasm					820:827	intraductal papillary mucinous neoplasm	789:827	intraductal papillary mucinous neoplasm (IPMN)	789:834	In order to assess this possibility for pancreatic cancer, we have performed an in-depth LC-MS/MS analysis of the proteome and MS(n)-based characterization of the N-linked glycome of a small set of pancreatic ductal fluid obtained from normal, pancreatitis, intraductal papillary mucinous neoplasm (IPMN), and pancreatic adenocarcinoma patients.
24328148	3	27	theme	glycoproteomic	475:488	arg1	changes					490:496	glycoproteomic changes	475:496	glycoproteomic changes	475:496	Protein glycosylation is altered in many cancers, leading many to propose that glycoproteomic changes may provide suitable biomarkers.
24328148	7	28	theme	glycoform	1313:1321	arg1	diversity					1323:1331	increased REG protein glycoform diversity	1291:1331	increased REG protein glycoform diversity in cancer	1291:1341	Validation of these changes in abundance by Western blotting revealed increased REG protein glycoform diversity in cancer.
24328148	3	29	theme	many	432:435	arg1	cancers					437:443	many cancers	432:443	many cancers	432:443	Protein glycosylation is altered in many cancers, leading many to propose that glycoproteomic changes may provide suitable biomarkers.
24328148	5	30	theme	ductal	965:970	arg1	fluid					972:976	cancer ductal fluid	958:976	cancer ductal fluid	958:976	Our results identify a set of seven proteins that were consistently increased in cancer ductal fluid compared to normal (AMYP, PRSS1, GP2-1, CCDC132, REG1A, REG1B, and REG3A) and one protein that was consistently decreased (LIPR2).
24328148	7	31	theme	REG	1301:1303	arg1	diversity					1323:1331	increased REG protein glycoform diversity	1291:1331	increased REG protein glycoform diversity in cancer	1291:1341	Validation of these changes in abundance by Western blotting revealed increased REG protein glycoform diversity in cancer.
24328148	8	32	theme	glycans	1525:1531	arg1	prevalence					1498:1507	the prevalence	1494:1507	the prevalence of six dominant glycans	1494:1531	Characterization of the total N-linked glycome of normal, IPMN, and adenocarcinoma ductal fluid clustered samples into three discrete groups based on the prevalence of six dominant glycans.
24328148	8	33	theme	total	1368:1372	arg1	glycome					1383:1389	the total N-linked glycome	1364:1389	the total N-linked glycome of normal, IPMN, and adenocarcinoma ductal fluid clustered samples into three discrete groups	1364:1483	Characterization of the total N-linked glycome of normal, IPMN, and adenocarcinoma ductal fluid clustered samples into three discrete groups based on the prevalence of six dominant glycans.
24328148	3	34	theme	suitable	510:517	arg1	biomarkers					519:528	suitable biomarkers	510:528	suitable biomarkers	510:528	Protein glycosylation is altered in many cancers, leading many to propose that glycoproteomic changes may provide suitable biomarkers.
24328148	3	35	gly	glycosylation	404:416	arg1	cancers					437:443	many cancers	432:443	many cancers	432:443	Protein glycosylation is altered in many cancers, leading many to propose that glycoproteomic changes may provide suitable biomarkers.
24328148	2	36	theme	high	196:199	arg1	mortality					201:209	The high mortality	192:209	The high mortality associated with adenocarcinoma of the pancreatic epithelium	192:269	The high mortality associated with adenocarcinoma of the pancreatic epithelium justifies the broadest possible search for new biomarkers that can facilitate early detection or monitor treatment efficacy.
24328148	8	37	theme	glycome	1383:1389	arg1	Characterization					1344:1359	Characterization	1344:1359	Characterization of the total N-linked glycome of normal, IPMN, and adenocarcinoma ductal fluid clustered samples into three discrete groups	1344:1483	Characterization of the total N-linked glycome of normal, IPMN, and adenocarcinoma ductal fluid clustered samples into three discrete groups based on the prevalence of six dominant glycans.
24328148	0	38	theme	normal	42:47	arg1	fluid					67:71	normal pancreatic ductal fluid	42:71	normal pancreatic ductal fluid	42:71	Discrimination between adenocarcinoma and normal pancreatic ductal fluid by proteomic and glycomic analysis.
24328148	4	39	dep	-based	663:668	arg1	MS					658:659	MS	658:659	MS	658:659	In order to assess this possibility for pancreatic cancer, we have performed an in-depth LC-MS/MS analysis of the proteome and MS(n)-based characterization of the N-linked glycome of a small set of pancreatic ductal fluid obtained from normal, pancreatitis, intraductal papillary mucinous neoplasm (IPMN), and pancreatic adenocarcinoma patients.
24328148	7	40	theme	Western	1265:1271	arg1	blotting					1273:1280	Western blotting	1265:1280	Western blotting	1265:1280	Validation of these changes in abundance by Western blotting revealed increased REG protein glycoform diversity in cancer.
24328148	4	41	theme	pancreatic	571:580	arg1	cancer					582:587	pancreatic cancer	571:587	pancreatic cancer	571:587	In order to assess this possibility for pancreatic cancer, we have performed an in-depth LC-MS/MS analysis of the proteome and MS(n)-based characterization of the N-linked glycome of a small set of pancreatic ductal fluid obtained from normal, pancreatitis, intraductal papillary mucinous neoplasm (IPMN), and pancreatic adenocarcinoma patients.
24328148	4	42	theme	pancreatic	841:850	arg1	patients					867:874	pancreatic adenocarcinoma patients	841:874	pancreatic adenocarcinoma patients	841:874	In order to assess this possibility for pancreatic cancer, we have performed an in-depth LC-MS/MS analysis of the proteome and MS(n)-based characterization of the N-linked glycome of a small set of pancreatic ductal fluid obtained from normal, pancreatitis, intraductal papillary mucinous neoplasm (IPMN), and pancreatic adenocarcinoma patients.
24328148	0	43	theme	ductal	60:65	arg1	fluid					67:71	normal pancreatic ductal fluid	42:71	normal pancreatic ductal fluid	42:71	Discrimination between adenocarcinoma and normal pancreatic ductal fluid by proteomic and glycomic analysis.
24328148	4	44	theme	adenocarcinoma	852:865	arg1	patients					867:874	pancreatic adenocarcinoma patients	841:874	pancreatic adenocarcinoma patients	841:874	In order to assess this possibility for pancreatic cancer, we have performed an in-depth LC-MS/MS analysis of the proteome and MS(n)-based characterization of the N-linked glycome of a small set of pancreatic ductal fluid obtained from normal, pancreatitis, intraductal papillary mucinous neoplasm (IPMN), and pancreatic adenocarcinoma patients.
24328148	10	45	theme	human	1865:1869	arg1	subjects					1871:1878	human subjects	1865:1878	human subjects	1865:1878	Our results emphasize that individual variation in protein glycosylation must be considered when assessing the value of a glycoproteomic marker, but also indicate that glycosylation diversity across human subjects can be reduced to simpler clusters of individuals whose N-linked glycans share structural features.
24328148	8	46	theme	IPMN	1402:1405	arg1	glycome					1383:1389	the total N-linked glycome	1364:1389	the total N-linked glycome of normal, IPMN, and adenocarcinoma ductal fluid clustered samples into three discrete groups	1364:1483	Characterization of the total N-linked glycome of normal, IPMN, and adenocarcinoma ductal fluid clustered samples into three discrete groups based on the prevalence of six dominant glycans.
24328148	7	47	theme	changes	1241:1247	arg1	Validation					1221:1230	Validation	1221:1230	Validation of these changes in abundance by Western blotting	1221:1280	Validation of these changes in abundance by Western blotting revealed increased REG protein glycoform diversity in cancer.
24328148	4	48	theme	proteome	645:652	arg1	characterization					670:685	MS(n)-based characterization	658:685	MS(n)-based characterization of the N-linked glycome of a small set of pancreatic ductal fluid obtained from normal, pancreatitis, intraductal papillary mucinous neoplasm (IPMN), and pancreatic adenocarcinoma patients	658:874	In order to assess this possibility for pancreatic cancer, we have performed an in-depth LC-MS/MS analysis of the proteome and MS(n)-based characterization of the N-linked glycome of a small set of pancreatic ductal fluid obtained from normal, pancreatitis, intraductal papillary mucinous neoplasm (IPMN), and pancreatic adenocarcinoma patients.
24328148	4	48	theme	proteome	645:652	arg1	analysis					629:636	an in-depth LC-MS/MS analysis	608:636	an in-depth LC-MS/MS analysis of the proteome	608:652	In order to assess this possibility for pancreatic cancer, we have performed an in-depth LC-MS/MS analysis of the proteome and MS(n)-based characterization of the N-linked glycome of a small set of pancreatic ductal fluid obtained from normal, pancreatitis, intraductal papillary mucinous neoplasm (IPMN), and pancreatic adenocarcinoma patients.
24328148	4	49	theme	LC-MS/MS	620:627	arg1	analysis					629:636	an in-depth LC-MS/MS analysis	608:636	an in-depth LC-MS/MS analysis of the proteome	608:652	In order to assess this possibility for pancreatic cancer, we have performed an in-depth LC-MS/MS analysis of the proteome and MS(n)-based characterization of the N-linked glycome of a small set of pancreatic ductal fluid obtained from normal, pancreatitis, intraductal papillary mucinous neoplasm (IPMN), and pancreatic adenocarcinoma patients.
24328148	8	50	theme	normal	1394:1399	arg1	glycome					1383:1389	the total N-linked glycome	1364:1389	the total N-linked glycome of normal, IPMN, and adenocarcinoma ductal fluid clustered samples into three discrete groups	1364:1483	Characterization of the total N-linked glycome of normal, IPMN, and adenocarcinoma ductal fluid clustered samples into three discrete groups based on the prevalence of six dominant glycans.
24328148	10	51	link	N-linked	1936:1943	arg1	glycans					1945:1951	N-linked glycans	1936:1951	N-linked glycans	1936:1951	Our results emphasize that individual variation in protein glycosylation must be considered when assessing the value of a glycoproteomic marker, but also indicate that glycosylation diversity across human subjects can be reduced to simpler clusters of individuals whose N-linked glycans share structural features.
24328148	7	52	from	Validation	1221:1230	arg1	abundance					1252:1260	abundance	1252:1260	abundance	1252:1260	Validation of these changes in abundance by Western blotting revealed increased REG protein glycoform diversity in cancer.
24328148	2	53	theme	new	314:316	arg1	biomarkers					318:327	new biomarkers	314:327	new biomarkers that can facilitate early detection or monitor treatment efficacy	314:393	The high mortality associated with adenocarcinoma of the pancreatic epithelium justifies the broadest possible search for new biomarkers that can facilitate early detection or monitor treatment efficacy.
24328148	9	54	theme	small	1644:1648	arg1	samples					1657:1663	samples	1657:1663	samples	1657:1663	Within each group, the profiles of less prevalent glycans were able to distinguish normal from cancer on this small set of samples.
24328148	9	54	theme	small	1644:1648	arg1	set					1650:1652	this small set	1639:1652	this small set of samples	1639:1663	Within each group, the profiles of less prevalent glycans were able to distinguish normal from cancer on this small set of samples.
24328148	10	55	theme	simpler	1898:1904	arg1	clusters					1906:1913	simpler clusters	1898:1913	simpler clusters of individuals whose N-linked glycans share structural features	1898:1977	Our results emphasize that individual variation in protein glycosylation must be considered when assessing the value of a glycoproteomic marker, but also indicate that glycosylation diversity across human subjects can be reduced to simpler clusters of individuals whose N-linked glycans share structural features.
24328148	10	56	from	variation	1704:1712	arg1	glycosylation					1725:1737	protein glycosylation	1717:1737	protein glycosylation	1717:1737	Our results emphasize that individual variation in protein glycosylation must be considered when assessing the value of a glycoproteomic marker, but also indicate that glycosylation diversity across human subjects can be reduced to simpler clusters of individuals whose N-linked glycans share structural features.
24328148	4	57	theme	N-linked	694:701	arg1	glycome					703:709	the N-linked glycome	690:709	the N-linked glycome of a small set of pancreatic ductal fluid obtained from normal, pancreatitis, intraductal papillary mucinous neoplasm (IPMN), and pancreatic adenocarcinoma patients	690:874	In order to assess this possibility for pancreatic cancer, we have performed an in-depth LC-MS/MS analysis of the proteome and MS(n)-based characterization of the N-linked glycome of a small set of pancreatic ductal fluid obtained from normal, pancreatitis, intraductal papillary mucinous neoplasm (IPMN), and pancreatic adenocarcinoma patients.
24328148	9	58	theme	glycans	1584:1590	arg1	able					1597:1600	able	1597:1600	able	1597:1600	Within each group, the profiles of less prevalent glycans were able to distinguish normal from cancer on this small set of samples.
24328148	9	58	theme	glycans	1584:1590	arg1	profiles					1557:1564	the profiles	1553:1564	the profiles of less prevalent glycans	1553:1590	Within each group, the profiles of less prevalent glycans were able to distinguish normal from cancer on this small set of samples.
24328148	8	59	theme	ductal	1427:1432	arg1	samples					1450:1456	adenocarcinoma ductal fluid clustered samples	1412:1456	adenocarcinoma ductal fluid clustered samples	1412:1456	Characterization of the total N-linked glycome of normal, IPMN, and adenocarcinoma ductal fluid clustered samples into three discrete groups based on the prevalence of six dominant glycans.
24328148	8	60	theme	clustered	1440:1448	arg1	samples					1450:1456	adenocarcinoma ductal fluid clustered samples	1412:1456	adenocarcinoma ductal fluid clustered samples	1412:1456	Characterization of the total N-linked glycome of normal, IPMN, and adenocarcinoma ductal fluid clustered samples into three discrete groups based on the prevalence of six dominant glycans.
24328148	4	61	link	N-linked	694:701	arg1	glycome					703:709	the N-linked glycome	690:709	the N-linked glycome of a small set of pancreatic ductal fluid obtained from normal, pancreatitis, intraductal papillary mucinous neoplasm (IPMN), and pancreatic adenocarcinoma patients	690:874	In order to assess this possibility for pancreatic cancer, we have performed an in-depth LC-MS/MS analysis of the proteome and MS(n)-based characterization of the N-linked glycome of a small set of pancreatic ductal fluid obtained from normal, pancreatitis, intraductal papillary mucinous neoplasm (IPMN), and pancreatic adenocarcinoma patients.
24328148	4	62	theme	-based	663:668	arg1	characterization					670:685	MS(n)-based characterization	658:685	MS(n)-based characterization of the N-linked glycome of a small set of pancreatic ductal fluid obtained from normal, pancreatitis, intraductal papillary mucinous neoplasm (IPMN), and pancreatic adenocarcinoma patients	658:874	In order to assess this possibility for pancreatic cancer, we have performed an in-depth LC-MS/MS analysis of the proteome and MS(n)-based characterization of the N-linked glycome of a small set of pancreatic ductal fluid obtained from normal, pancreatitis, intraductal papillary mucinous neoplasm (IPMN), and pancreatic adenocarcinoma patients.
24328148	2	63	theme	pancreatic	249:258	arg1	epithelium					260:269	the pancreatic epithelium	245:269	the pancreatic epithelium	245:269	The high mortality associated with adenocarcinoma of the pancreatic epithelium justifies the broadest possible search for new biomarkers that can facilitate early detection or monitor treatment efficacy.
24328148	1	64	theme	specific	123:130	arg1	biomarkers					132:141	Sensitive and specific biomarkers	109:141	Sensitive and specific biomarkers for pancreatic cancer	109:163	Sensitive and specific biomarkers for pancreatic cancer are currently unavailable.
24328148	10	65	theme	structural	1959:1968	arg1	features					1970:1977	structural features	1959:1977	structural features	1959:1977	Our results emphasize that individual variation in protein glycosylation must be considered when assessing the value of a glycoproteomic marker, but also indicate that glycosylation diversity across human subjects can be reduced to simpler clusters of individuals whose N-linked glycans share structural features.
24328148	8	66	theme	discrete	1469:1476	arg1	groups					1478:1483	three discrete groups	1463:1483	three discrete groups	1463:1483	Characterization of the total N-linked glycome of normal, IPMN, and adenocarcinoma ductal fluid clustered samples into three discrete groups based on the prevalence of six dominant glycans.
24328148	5	67	dep	normal	990:995	arg1	GP2-1					1011:1015	GP2-1	1011:1015	GP2-1	1011:1015	Our results identify a set of seven proteins that were consistently increased in cancer ductal fluid compared to normal (AMYP, PRSS1, GP2-1, CCDC132, REG1A, REG1B, and REG3A) and one protein that was consistently decreased (LIPR2).
24328148	5	67	dep	normal	990:995	arg1	REG1B					1034:1038	REG1B	1034:1038	REG1B	1034:1038	Our results identify a set of seven proteins that were consistently increased in cancer ductal fluid compared to normal (AMYP, PRSS1, GP2-1, CCDC132, REG1A, REG1B, and REG3A) and one protein that was consistently decreased (LIPR2).
24328148	5	67	dep	normal	990:995	arg1	AMYP					998:1001	AMYP	998:1001	AMYP	998:1001	Our results identify a set of seven proteins that were consistently increased in cancer ductal fluid compared to normal (AMYP, PRSS1, GP2-1, CCDC132, REG1A, REG1B, and REG3A) and one protein that was consistently decreased (LIPR2).
24328148	5	67	dep	normal	990:995	arg1	CCDC132					1018:1024	CCDC132	1018:1024	CCDC132	1018:1024	Our results identify a set of seven proteins that were consistently increased in cancer ductal fluid compared to normal (AMYP, PRSS1, GP2-1, CCDC132, REG1A, REG1B, and REG3A) and one protein that was consistently decreased (LIPR2).
24328148	5	67	dep	normal	990:995	arg1	REG3A					1045:1049	REG3A	1045:1049	REG3A	1045:1049	Our results identify a set of seven proteins that were consistently increased in cancer ductal fluid compared to normal (AMYP, PRSS1, GP2-1, CCDC132, REG1A, REG1B, and REG3A) and one protein that was consistently decreased (LIPR2).
24328148	5	67	dep	normal	990:995	arg1	REG1A					1027:1031	REG1A	1027:1031	REG1A	1027:1031	Our results identify a set of seven proteins that were consistently increased in cancer ductal fluid compared to normal (AMYP, PRSS1, GP2-1, CCDC132, REG1A, REG1B, and REG3A) and one protein that was consistently decreased (LIPR2).
24328148	5	67	dep	normal	990:995	arg1	PRSS1					1004:1008	PRSS1	1004:1008	PRSS1	1004:1008	Our results identify a set of seven proteins that were consistently increased in cancer ductal fluid compared to normal (AMYP, PRSS1, GP2-1, CCDC132, REG1A, REG1B, and REG3A) and one protein that was consistently decreased (LIPR2).
24328148	0	68	theme	proteomic	76:84	arg1	analysis					99:106	proteomic and glycomic analysis	76:106	proteomic and glycomic analysis	76:106	Discrimination between adenocarcinoma and normal pancreatic ductal fluid by proteomic and glycomic analysis.
24328148	2	69	theme	broadest	285:292	arg1	search					303:308	the broadest possible search	281:308	the broadest possible search for new biomarkers that can facilitate early detection or monitor treatment efficacy	281:393	The high mortality associated with adenocarcinoma of the pancreatic epithelium justifies the broadest possible search for new biomarkers that can facilitate early detection or monitor treatment efficacy.
24328148	0	70	theme	glycomic	90:97	arg1	analysis					99:106	proteomic and glycomic analysis	76:106	proteomic and glycomic analysis	76:106	Discrimination between adenocarcinoma and normal pancreatic ductal fluid by proteomic and glycomic analysis.
24328148	5	71	theme	proteins	913:920	arg1	proteins					913:920	seven proteins	907:920	seven proteins that were consistently increased in cancer ductal fluid compared to normal (AMYP, PRSS1, GP2-1, CCDC132, REG1A, REG1B, and REG3A) and one protein that was consistently decreased (LIPR2)	907:1106	Our results identify a set of seven proteins that were consistently increased in cancer ductal fluid compared to normal (AMYP, PRSS1, GP2-1, CCDC132, REG1A, REG1B, and REG3A) and one protein that was consistently decreased (LIPR2).
24328148	5	71	theme	proteins	913:920	arg1	set					900:902	a set	898:902	a set of seven proteins that were consistently increased in cancer ductal fluid compared to normal (AMYP, PRSS1, GP2-1, CCDC132, REG1A, REG1B, and REG3A) and one protein that was consistently decreased (LIPR2)	898:1106	Our results identify a set of seven proteins that were consistently increased in cancer ductal fluid compared to normal (AMYP, PRSS1, GP2-1, CCDC132, REG1A, REG1B, and REG3A) and one protein that was consistently decreased (LIPR2).
24328148	4	72	theme	small	716:720	arg1	set					722:724	a small set	714:724	a small set of pancreatic ductal fluid obtained from normal, pancreatitis, intraductal papillary mucinous neoplasm (IPMN), and pancreatic adenocarcinoma patients	714:874	In order to assess this possibility for pancreatic cancer, we have performed an in-depth LC-MS/MS analysis of the proteome and MS(n)-based characterization of the N-linked glycome of a small set of pancreatic ductal fluid obtained from normal, pancreatitis, intraductal papillary mucinous neoplasm (IPMN), and pancreatic adenocarcinoma patients.
24328148	4	72	theme	small	716:720	arg1	fluid					747:751	pancreatic ductal fluid	729:751	pancreatic ductal fluid obtained from normal, pancreatitis, intraductal papillary mucinous neoplasm (IPMN), and pancreatic adenocarcinoma patients	729:874	In order to assess this possibility for pancreatic cancer, we have performed an in-depth LC-MS/MS analysis of the proteome and MS(n)-based characterization of the N-linked glycome of a small set of pancreatic ductal fluid obtained from normal, pancreatitis, intraductal papillary mucinous neoplasm (IPMN), and pancreatic adenocarcinoma patients.
24328148	10	73	theme	marker	1803:1808	arg1	value					1777:1781	the value	1773:1781	the value of a glycoproteomic marker	1773:1808	Our results emphasize that individual variation in protein glycosylation must be considered when assessing the value of a glycoproteomic marker, but also indicate that glycosylation diversity across human subjects can be reduced to simpler clusters of individuals whose N-linked glycans share structural features.
24328148	7	74	theme	protein	1305:1311	arg1	diversity					1323:1331	increased REG protein glycoform diversity	1291:1331	increased REG protein glycoform diversity in cancer	1291:1341	Validation of these changes in abundance by Western blotting revealed increased REG protein glycoform diversity in cancer.
24328148	3	75	theme	Protein	396:402	arg1	glycosylation					404:416	Protein glycosylation	396:416	Protein glycosylation	396:416	Protein glycosylation is altered in many cancers, leading many to propose that glycoproteomic changes may provide suitable biomarkers.
24328148	8	76	theme	dominant	1516:1523	arg1	glycans					1525:1531	six dominant glycans	1512:1531	six dominant glycans	1512:1531	Characterization of the total N-linked glycome of normal, IPMN, and adenocarcinoma ductal fluid clustered samples into three discrete groups based on the prevalence of six dominant glycans.
24328148	4	77	theme	intraductal	789:799	arg1	IPMN					830:833	IPMN	830:833	IPMN	830:833	In order to assess this possibility for pancreatic cancer, we have performed an in-depth LC-MS/MS analysis of the proteome and MS(n)-based characterization of the N-linked glycome of a small set of pancreatic ductal fluid obtained from normal, pancreatitis, intraductal papillary mucinous neoplasm (IPMN), and pancreatic adenocarcinoma patients.
24328148	4	77	theme	intraductal	789:799	arg1	neoplasm					820:827	intraductal papillary mucinous neoplasm	789:827	intraductal papillary mucinous neoplasm (IPMN)	789:834	In order to assess this possibility for pancreatic cancer, we have performed an in-depth LC-MS/MS analysis of the proteome and MS(n)-based characterization of the N-linked glycome of a small set of pancreatic ductal fluid obtained from normal, pancreatitis, intraductal papillary mucinous neoplasm (IPMN), and pancreatic adenocarcinoma patients.
24328148	5	78	theme	cancer	958:963	arg1	fluid					972:976	cancer ductal fluid	958:976	cancer ductal fluid	958:976	Our results identify a set of seven proteins that were consistently increased in cancer ductal fluid compared to normal (AMYP, PRSS1, GP2-1, CCDC132, REG1A, REG1B, and REG3A) and one protein that was consistently decreased (LIPR2).
24328148	7	79	theme	increased	1291:1299	arg1	diversity					1323:1331	increased REG protein glycoform diversity	1291:1331	increased REG protein glycoform diversity in cancer	1291:1341	Validation of these changes in abundance by Western blotting revealed increased REG protein glycoform diversity in cancer.
24328148	4	80	dep	order	534:538	arg1	assess					543:548	assess	543:548	to assess this possibility for pancreatic cancer	540:587	In order to assess this possibility for pancreatic cancer, we have performed an in-depth LC-MS/MS analysis of the proteome and MS(n)-based characterization of the N-linked glycome of a small set of pancreatic ductal fluid obtained from normal, pancreatitis, intraductal papillary mucinous neoplasm (IPMN), and pancreatic adenocarcinoma patients.
24328148	1	81	theme	Sensitive	109:117	arg1	biomarkers					132:141	Sensitive and specific biomarkers	109:141	Sensitive and specific biomarkers for pancreatic cancer	109:163	Sensitive and specific biomarkers for pancreatic cancer are currently unavailable.
24328148	5	82	dep	decreased	1090:1098	arg1	LIPR2					1101:1105	LIPR2	1101:1105	LIPR2	1101:1105	Our results identify a set of seven proteins that were consistently increased in cancer ductal fluid compared to normal (AMYP, PRSS1, GP2-1, CCDC132, REG1A, REG1B, and REG3A) and one protein that was consistently decreased (LIPR2).
24328148	10	83	theme	glycosylation	1834:1846	arg1	diversity					1848:1856	glycosylation diversity	1834:1856	glycosylation diversity across human subjects	1834:1878	Our results emphasize that individual variation in protein glycosylation must be considered when assessing the value of a glycoproteomic marker, but also indicate that glycosylation diversity across human subjects can be reduced to simpler clusters of individuals whose N-linked glycans share structural features.
24328148	0	84	theme	pancreatic	49:58	arg1	fluid					67:71	normal pancreatic ductal fluid	42:71	normal pancreatic ductal fluid	42:71	Discrimination between adenocarcinoma and normal pancreatic ductal fluid by proteomic and glycomic analysis.
24328148	8	85	theme	N-linked	1374:1381	arg1	glycome					1383:1389	the total N-linked glycome	1364:1389	the total N-linked glycome of normal, IPMN, and adenocarcinoma ductal fluid clustered samples into three discrete groups	1364:1483	Characterization of the total N-linked glycome of normal, IPMN, and adenocarcinoma ductal fluid clustered samples into three discrete groups based on the prevalence of six dominant glycans.
24328148	9	86	theme	prevalent	1574:1582	arg1	glycans					1584:1590	less prevalent glycans	1569:1590	less prevalent glycans	1569:1590	Within each group, the profiles of less prevalent glycans were able to distinguish normal from cancer on this small set of samples.
24328148	2	87	theme	treatment	376:384	arg1	efficacy					386:393	treatment efficacy	376:393	treatment efficacy	376:393	The high mortality associated with adenocarcinoma of the pancreatic epithelium justifies the broadest possible search for new biomarkers that can facilitate early detection or monitor treatment efficacy.
24328148	6	88	theme	normal	1196:1201	arg1	cells					1214:1218	normal pancreatic cells	1196:1218	normal pancreatic cells	1196:1218	These proteins are all directly or indirectly associated with the secretory pathway in normal pancreatic cells.
24328148	4	89	theme	mucinous	811:818	arg1	IPMN					830:833	IPMN	830:833	IPMN	830:833	In order to assess this possibility for pancreatic cancer, we have performed an in-depth LC-MS/MS analysis of the proteome and MS(n)-based characterization of the N-linked glycome of a small set of pancreatic ductal fluid obtained from normal, pancreatitis, intraductal papillary mucinous neoplasm (IPMN), and pancreatic adenocarcinoma patients.
24328148	4	89	theme	mucinous	811:818	arg1	neoplasm					820:827	intraductal papillary mucinous neoplasm	789:827	intraductal papillary mucinous neoplasm (IPMN)	789:834	In order to assess this possibility for pancreatic cancer, we have performed an in-depth LC-MS/MS analysis of the proteome and MS(n)-based characterization of the N-linked glycome of a small set of pancreatic ductal fluid obtained from normal, pancreatitis, intraductal papillary mucinous neoplasm (IPMN), and pancreatic adenocarcinoma patients.
24328148	10	90	theme	individual	1693:1702	arg1	variation					1704:1712	individual variation	1693:1712	individual variation in protein glycosylation	1693:1737	Our results emphasize that individual variation in protein glycosylation must be considered when assessing the value of a glycoproteomic marker, but also indicate that glycosylation diversity across human subjects can be reduced to simpler clusters of individuals whose N-linked glycans share structural features.
25104038	8	0	theme	domains	1273:1279	arg1	structures					1239:1248	The crystal structures	1227:1248	The crystal structures of the HbCLP1-isolated domains	1227:1279	The crystal structures of the HbCLP1-isolated domains were determined at high resolution.
25104038	11	1	theme	antifungal	1800:1809	arg1	activity					1811:1818	antifungal activity	1800:1818	antifungal activity	1800:1818	HbCLP1 and HbCLP2 were highly thermostable and exhibited antifungal activity against Alternaria alternata, suggesting their participation in plant defense mechanisms.
25104038	1	2	theme	chitinase-like	160:173	arg1	CLPs					185:188	CLPs	185:188	CLPs	185:188	Plants express chitinase and chitinase-like proteins (CLPs) belonging to the glycosyl hydrolases of the GH18 and GH19 families, which exhibit varied functions.
25104038	1	2	theme	chitinase-like	160:173	arg1	proteins					175:182	chitinase-like proteins	160:182	chitinase-like proteins (CLPs) belonging to the glycosyl hydrolases of the GH18 and GH19 families, which exhibit varied functions	160:288	Plants express chitinase and chitinase-like proteins (CLPs) belonging to the glycosyl hydrolases of the GH18 and GH19 families, which exhibit varied functions.
25104038	2	3	from	CLPs	291:294	arg1	family					308:313	the GH18 family	299:313	the GH18 family	299:313	CLPs in the GH18 family have been structurally and functionally characterized; however, there are no structures available for any member of the GH19 family.
25104038	1	4	theme	GH19	244:247	arg1	families					249:256	the GH18 and GH19 families	231:256	the GH18 and GH19 families	231:256	Plants express chitinase and chitinase-like proteins (CLPs) belonging to the glycosyl hydrolases of the GH18 and GH19 families, which exhibit varied functions.
25104038	0	5	from	study	12:16	arg1	brasiliensis					65:76	Hevea brasiliensis	59:76	Hevea brasiliensis	59:76	Comparative study of two GH19 chitinase-like proteins from Hevea brasiliensis, one exhibiting a novel carbohydrate-binding domain.
25104038	4	6	theme	b	634:634	arg1	11.0101					636:642	the allergen Hev b 11.0101	617:642	the allergen Hev b 11.0101 previously described by others	617:673	HbCLP1 was identical to the allergen Hev b 11.0101 previously described by others, while HbCLP2 was a novel isoform exhibiting an unusual half chitin-binding domain before the catalytic domain.
25104038	1	7	theme	families	249:256	arg1	hydrolases					217:226	the glycosyl hydrolases	204:226	the glycosyl hydrolases	204:226	Plants express chitinase and chitinase-like proteins (CLPs) belonging to the glycosyl hydrolases of the GH18 and GH19 families, which exhibit varied functions.
25104038	10	8	from	donor	1630:1634	arg1	residues					1691:1698	the in silico mutated residues	1669:1698	the in silico mutated residues	1669:1698	Endochitinase activity was restored in both proteins by mutating residues A117E (HbCLP1) and A147E (HbCLP2); the distance between the catalytic proton donor and the catalytic nucleophile in the in silico mutated residues was 9.5 Å, as occurs in inverting enzymes.
25104038	4	9	theme	novel	695:699	arg1	HbCLP2					682:687	HbCLP2	682:687	HbCLP2	682:687	HbCLP1 was identical to the allergen Hev b 11.0101 previously described by others, while HbCLP2 was a novel isoform exhibiting an unusual half chitin-binding domain before the catalytic domain.
25104038	4	9	theme	novel	695:699	arg1	isoform					701:707	a novel isoform	693:707	a novel isoform exhibiting an unusual half chitin-binding domain before the catalytic domain	693:784	HbCLP1 was identical to the allergen Hev b 11.0101 previously described by others, while HbCLP2 was a novel isoform exhibiting an unusual half chitin-binding domain before the catalytic domain.
25104038	6	10	theme	high	1041:1044	arg1	affinities					1046:1055	high affinities	1041:1055	high affinities	1041:1055	Nonetheless, both CLPs bound chitin and chitotriose (GlcNAc)3 with high affinities, as evaluated with chitin-affinity chromatography and tryptophan fluorescence experiments.
25104038	9	11	theme	sugar	1464:1468	arg1	binding					1470:1476	sugar binding	1464:1476	sugar binding	1464:1476	The analysis of the crystallographic models and docking experiments using (GlcNAc)6 oligosaccharides provides evidence of the residues involved in sugar binding.
25104038	6	12	theme	chitin-affinity	1076:1090	arg1	chromatography					1092:1105	chitin-affinity chromatography	1076:1105	chitin-affinity chromatography	1076:1105	Nonetheless, both CLPs bound chitin and chitotriose (GlcNAc)3 with high affinities, as evaluated with chitin-affinity chromatography and tryptophan fluorescence experiments.
25104038	3	13	theme	rubber	500:505	arg1	brasiliensis					518:529	the rubber tree Hevea brasiliensis	496:529	the rubber tree Hevea brasiliensis (HbCLP1 and HbCLP2)	496:549	In this study, two CLPs of the GH19 family from the rubber tree Hevea brasiliensis (HbCLP1 and HbCLP2) were cloned, expressed and characterized.
25104038	7	14	theme	higher	1208:1213	arg1	affinities					1215:1224	even higher affinities	1203:1224	even higher affinities	1203:1224	The chitin-binding domains also bound chitotriose with even higher affinities.
25104038	0	15	theme	carbohydrate-binding	102:121	arg1	domain					123:128	a novel carbohydrate-binding domain	94:128	a novel carbohydrate-binding domain	94:128	Comparative study of two GH19 chitinase-like proteins from Hevea brasiliensis, one exhibiting a novel carbohydrate-binding domain.
25104038	9	16	theme	residues	1443:1450	arg1	evidence					1427:1434	evidence	1427:1434	evidence of the residues involved in sugar binding	1427:1476	The analysis of the crystallographic models and docking experiments using (GlcNAc)6 oligosaccharides provides evidence of the residues involved in sugar binding.
25104038	10	17	theme	catalytic	1613:1621	arg1	donor					1630:1634	the catalytic proton donor	1609:1634	the catalytic proton donor	1609:1634	Endochitinase activity was restored in both proteins by mutating residues A117E (HbCLP1) and A147E (HbCLP2); the distance between the catalytic proton donor and the catalytic nucleophile in the in silico mutated residues was 9.5 Å, as occurs in inverting enzymes.
25104038	3	18	theme	family	484:489	arg1	CLPs					467:470	two CLPs	463:470	two CLPs of the GH19 family from the rubber tree Hevea brasiliensis (HbCLP1 and HbCLP2)	463:549	In this study, two CLPs of the GH19 family from the rubber tree Hevea brasiliensis (HbCLP1 and HbCLP2) were cloned, expressed and characterized.
25104038	9	19	theme	GlcNAc	1392:1397	arg1	oligosaccharides					1401:1416	(GlcNAc)6 oligosaccharides	1391:1416	(GlcNAc)6 oligosaccharides	1391:1416	The analysis of the crystallographic models and docking experiments using (GlcNAc)6 oligosaccharides provides evidence of the residues involved in sugar binding.
25104038	0	20	theme	novel	96:100	arg1	domain					123:128	a novel carbohydrate-binding domain	94:128	a novel carbohydrate-binding domain	94:128	Comparative study of two GH19 chitinase-like proteins from Hevea brasiliensis, one exhibiting a novel carbohydrate-binding domain.
25104038	5	21	theme	catalytic	843:851	arg1	residues					853:860	the catalytic residues Glu117 and Glu147	839:878	residues	853:860	Sequence alignments showed that in the two proteins the catalytic residues Glu117 and Glu147 in HbCLP1 and HbCLP2, respectively, were mutated to Ala, accounting for the lack of activity.
25104038	9	22	theme	docking	1365:1371	arg1	experiments					1373:1383	docking experiments	1365:1383	docking experiments	1365:1383	The analysis of the crystallographic models and docking experiments using (GlcNAc)6 oligosaccharides provides evidence of the residues involved in sugar binding.
25104038	8	23	theme	high	1300:1303	arg1	resolution					1305:1314	high resolution	1300:1314	high resolution	1300:1314	The crystal structures of the HbCLP1-isolated domains were determined at high resolution.
25104038	3	24	dep	brasiliensis	518:529	arg1	HbCLP2					543:548	HbCLP2	543:548	HbCLP2	543:548	In this study, two CLPs of the GH19 family from the rubber tree Hevea brasiliensis (HbCLP1 and HbCLP2) were cloned, expressed and characterized.
25104038	3	24	dep	brasiliensis	518:529	arg1	HbCLP1					532:537	HbCLP1	532:537	HbCLP1	532:537	In this study, two CLPs of the GH19 family from the rubber tree Hevea brasiliensis (HbCLP1 and HbCLP2) were cloned, expressed and characterized.
25104038	10	25	dep	in	1673:1674	arg1	silico					1676:1681	silico	1676:1681	silico	1676:1681	Endochitinase activity was restored in both proteins by mutating residues A117E (HbCLP1) and A147E (HbCLP2); the distance between the catalytic proton donor and the catalytic nucleophile in the in silico mutated residues was 9.5 Å, as occurs in inverting enzymes.
25104038	10	26	from	nucleophile	1654:1664	arg1	residues					1691:1698	the in silico mutated residues	1669:1698	the in silico mutated residues	1669:1698	Endochitinase activity was restored in both proteins by mutating residues A117E (HbCLP1) and A147E (HbCLP2); the distance between the catalytic proton donor and the catalytic nucleophile in the in silico mutated residues was 9.5 Å, as occurs in inverting enzymes.
25104038	2	27	theme	GH18	303:306	arg1	family					308:313	the GH18 family	299:313	the GH18 family	299:313	CLPs in the GH18 family have been structurally and functionally characterized; however, there are no structures available for any member of the GH19 family.
25104038	11	28	theme	Alternaria	1828:1837	arg1	alternata					1839:1847	Alternaria alternata	1828:1847	Alternaria alternata	1828:1847	HbCLP1 and HbCLP2 were highly thermostable and exhibited antifungal activity against Alternaria alternata, suggesting their participation in plant defense mechanisms.
25104038	0	29	theme	Comparative	0:10	arg1	one					79:81	one	79:81	one	79:81	Comparative study of two GH19 chitinase-like proteins from Hevea brasiliensis, one exhibiting a novel carbohydrate-binding domain.
25104038	0	29	theme	Comparative	0:10	arg1	study					12:16	Comparative study	0:16	Comparative study of two GH19 chitinase-like proteins from Hevea brasiliensis	0:76	Comparative study of two GH19 chitinase-like proteins from Hevea brasiliensis, one exhibiting a novel carbohydrate-binding domain.
25104038	6	30	theme	tryptophan	1111:1120	arg1	experiments					1135:1145	tryptophan fluorescence experiments	1111:1145	tryptophan fluorescence experiments	1111:1145	Nonetheless, both CLPs bound chitin and chitotriose (GlcNAc)3 with high affinities, as evaluated with chitin-affinity chromatography and tryptophan fluorescence experiments.
25104038	5	31	from	residues	853:860	arg1	HbCLP2					894:899	HbCLP2	894:899	HbCLP2	894:899	Sequence alignments showed that in the two proteins the catalytic residues Glu117 and Glu147 in HbCLP1 and HbCLP2, respectively, were mutated to Ala, accounting for the lack of activity.
25104038	5	31	from	residues	853:860	arg1	HbCLP1					883:888	HbCLP1	883:888	HbCLP1	883:888	Sequence alignments showed that in the two proteins the catalytic residues Glu117 and Glu147 in HbCLP1 and HbCLP2, respectively, were mutated to Ala, accounting for the lack of activity.
25104038	1	32	theme	varied	273:278	arg1	functions					280:288	varied functions	273:288	varied functions	273:288	Plants express chitinase and chitinase-like proteins (CLPs) belonging to the glycosyl hydrolases of the GH18 and GH19 families, which exhibit varied functions.
25104038	3	33	theme	tree	507:510	arg1	brasiliensis					518:529	the rubber tree Hevea brasiliensis	496:529	the rubber tree Hevea brasiliensis (HbCLP1 and HbCLP2)	496:549	In this study, two CLPs of the GH19 family from the rubber tree Hevea brasiliensis (HbCLP1 and HbCLP2) were cloned, expressed and characterized.
25104038	10	34	theme	proton	1623:1628	arg1	donor					1630:1634	the catalytic proton donor	1609:1634	the catalytic proton donor	1609:1634	Endochitinase activity was restored in both proteins by mutating residues A117E (HbCLP1) and A147E (HbCLP2); the distance between the catalytic proton donor and the catalytic nucleophile in the in silico mutated residues was 9.5 Å, as occurs in inverting enzymes.
25104038	11	35	theme	defense	1890:1896	arg1	mechanisms					1898:1907	plant defense mechanisms	1884:1907	plant defense mechanisms	1884:1907	HbCLP1 and HbCLP2 were highly thermostable and exhibited antifungal activity against Alternaria alternata, suggesting their participation in plant defense mechanisms.
25104038	5	36	dep	residues	853:860	arg1	Glu117					862:867	Glu117	862:867	Glu117	862:867	Sequence alignments showed that in the two proteins the catalytic residues Glu117 and Glu147 in HbCLP1 and HbCLP2, respectively, were mutated to Ala, accounting for the lack of activity.
25104038	9	37	dep	models	1354:1359	arg1	the					1333:1335	the	1333:1335	the	1333:1335	The analysis of the crystallographic models and docking experiments using (GlcNAc)6 oligosaccharides provides evidence of the residues involved in sugar binding.
25104038	3	38	theme	Hevea	512:516	arg1	brasiliensis					518:529	the rubber tree Hevea brasiliensis	496:529	the rubber tree Hevea brasiliensis (HbCLP1 and HbCLP2)	496:549	In this study, two CLPs of the GH19 family from the rubber tree Hevea brasiliensis (HbCLP1 and HbCLP2) were cloned, expressed and characterized.
25104038	2	39	theme	available	403:411	arg1	structures					392:401	no structures	389:401	no structures available for any member of the GH19 family	389:445	CLPs in the GH18 family have been structurally and functionally characterized; however, there are no structures available for any member of the GH19 family.
25104038	0	40	theme	chitinase-like	30:43	arg1	proteins					45:52	two GH19 chitinase-like proteins	21:52	two GH19 chitinase-like proteins from Hevea brasiliensis	21:76	Comparative study of two GH19 chitinase-like proteins from Hevea brasiliensis, one exhibiting a novel carbohydrate-binding domain.
25104038	10	41	dep	residues	1544:1551	arg1	HbCLP1					1560:1565	HbCLP1	1560:1565	HbCLP1	1560:1565	Endochitinase activity was restored in both proteins by mutating residues A117E (HbCLP1) and A147E (HbCLP2); the distance between the catalytic proton donor and the catalytic nucleophile in the in silico mutated residues was 9.5 Å, as occurs in inverting enzymes.
25104038	10	41	dep	residues	1544:1551	arg1	A147E					1572:1576	A147E	1572:1576	A147E (HbCLP2)	1572:1585	Endochitinase activity was restored in both proteins by mutating residues A117E (HbCLP1) and A147E (HbCLP2); the distance between the catalytic proton donor and the catalytic nucleophile in the in silico mutated residues was 9.5 Å, as occurs in inverting enzymes.
25104038	10	41	dep	residues	1544:1551	arg1	A117E					1553:1557	A117E	1553:1557	A117E (HbCLP1)	1553:1566	Endochitinase activity was restored in both proteins by mutating residues A117E (HbCLP1) and A147E (HbCLP2); the distance between the catalytic proton donor and the catalytic nucleophile in the in silico mutated residues was 9.5 Å, as occurs in inverting enzymes.
25104038	10	41	dep	residues	1544:1551	arg1	residues					1544:1551	residues A117E (HbCLP1) and A147E (HbCLP2)	1544:1585	residues A117E (HbCLP1) and A147E (HbCLP2)	1544:1585	Endochitinase activity was restored in both proteins by mutating residues A117E (HbCLP1) and A147E (HbCLP2); the distance between the catalytic proton donor and the catalytic nucleophile in the in silico mutated residues was 9.5 Å, as occurs in inverting enzymes.
25104038	10	41	dep	residues	1544:1551	arg1	HbCLP2					1579:1584	HbCLP2	1579:1584	HbCLP2	1579:1584	Endochitinase activity was restored in both proteins by mutating residues A117E (HbCLP1) and A147E (HbCLP2); the distance between the catalytic proton donor and the catalytic nucleophile in the in silico mutated residues was 9.5 Å, as occurs in inverting enzymes.
25104038	5	42	theme	Sequence	787:794	arg1	alignments					796:805	Sequence alignments	787:805	Sequence alignments	787:805	Sequence alignments showed that in the two proteins the catalytic residues Glu117 and Glu147 in HbCLP1 and HbCLP2, respectively, were mutated to Ala, accounting for the lack of activity.
25104038	8	43	theme	HbCLP1-isolated	1257:1271	arg1	domains					1273:1279	the HbCLP1-isolated domains	1253:1279	the HbCLP1-isolated domains	1253:1279	The crystal structures of the HbCLP1-isolated domains were determined at high resolution.
25104038	1	44	theme	glycosyl	208:215	arg1	hydrolases					217:226	the glycosyl hydrolases	204:226	the glycosyl hydrolases	204:226	Plants express chitinase and chitinase-like proteins (CLPs) belonging to the glycosyl hydrolases of the GH18 and GH19 families, which exhibit varied functions.
25104038	0	45	theme	GH19	25:28	arg1	proteins					45:52	two GH19 chitinase-like proteins	21:52	two GH19 chitinase-like proteins from Hevea brasiliensis	21:76	Comparative study of two GH19 chitinase-like proteins from Hevea brasiliensis, one exhibiting a novel carbohydrate-binding domain.
25104038	9	46	theme	experiments	1373:1383	arg1	analysis					1321:1328	The analysis	1317:1328	The analysis of the crystallographic models and docking experiments using (GlcNAc)6 oligosaccharides	1317:1416	The analysis of the crystallographic models and docking experiments using (GlcNAc)6 oligosaccharides provides evidence of the residues involved in sugar binding.
25104038	0	47	from	brasiliensis	65:76	arg1	one					79:81	one	79:81	one	79:81	Comparative study of two GH19 chitinase-like proteins from Hevea brasiliensis, one exhibiting a novel carbohydrate-binding domain.
25104038	0	47	from	brasiliensis	65:76	arg1	proteins					45:52	two GH19 chitinase-like proteins	21:52	two GH19 chitinase-like proteins from Hevea brasiliensis	21:76	Comparative study of two GH19 chitinase-like proteins from Hevea brasiliensis, one exhibiting a novel carbohydrate-binding domain.
25104038	0	47	from	brasiliensis	65:76	arg1	study					12:16	Comparative study	0:16	Comparative study of two GH19 chitinase-like proteins from Hevea brasiliensis	0:76	Comparative study of two GH19 chitinase-like proteins from Hevea brasiliensis, one exhibiting a novel carbohydrate-binding domain.
25104038	9	48	theme	models	1354:1359	arg1	analysis					1321:1328	The analysis	1317:1328	The analysis of the crystallographic models and docking experiments using (GlcNAc)6 oligosaccharides	1317:1416	The analysis of the crystallographic models and docking experiments using (GlcNAc)6 oligosaccharides provides evidence of the residues involved in sugar binding.
25104038	2	49	theme	family	440:445	arg1	member					421:426	any member	417:426	any member of the GH19 family	417:445	CLPs in the GH18 family have been structurally and functionally characterized; however, there are no structures available for any member of the GH19 family.
25104038	4	50	theme	catalytic	769:777	arg1	domain					779:784	the catalytic domain	765:784	the catalytic domain	765:784	HbCLP1 was identical to the allergen Hev b 11.0101 previously described by others, while HbCLP2 was a novel isoform exhibiting an unusual half chitin-binding domain before the catalytic domain.
25104038	11	51	from	participation	1867:1879	arg1	mechanisms					1898:1907	plant defense mechanisms	1884:1907	plant defense mechanisms	1884:1907	HbCLP1 and HbCLP2 were highly thermostable and exhibited antifungal activity against Alternaria alternata, suggesting their participation in plant defense mechanisms.
25104038	8	52	theme	crystal	1231:1237	arg1	structures					1239:1248	The crystal structures	1227:1248	The crystal structures of the HbCLP1-isolated domains	1227:1279	The crystal structures of the HbCLP1-isolated domains were determined at high resolution.
25104038	0	53	theme	proteins	45:52	arg1	one					79:81	one	79:81	one	79:81	Comparative study of two GH19 chitinase-like proteins from Hevea brasiliensis, one exhibiting a novel carbohydrate-binding domain.
25104038	0	53	theme	proteins	45:52	arg1	study					12:16	Comparative study	0:16	Comparative study of two GH19 chitinase-like proteins from Hevea brasiliensis	0:76	Comparative study of two GH19 chitinase-like proteins from Hevea brasiliensis, one exhibiting a novel carbohydrate-binding domain.
25104038	10	54	theme	Endochitinase	1479:1491	arg1	activity					1493:1500	Endochitinase activity	1479:1500	Endochitinase activity	1479:1500	Endochitinase activity was restored in both proteins by mutating residues A117E (HbCLP1) and A147E (HbCLP2); the distance between the catalytic proton donor and the catalytic nucleophile in the in silico mutated residues was 9.5 Å, as occurs in inverting enzymes.
25104038	2	55	theme	GH19	435:438	arg1	family					440:445	the GH19 family	431:445	the GH19 family	431:445	CLPs in the GH18 family have been structurally and functionally characterized; however, there are no structures available for any member of the GH19 family.
25104038	10	56	theme	mutated	1683:1689	arg1	residues					1691:1698	the in silico mutated residues	1669:1698	the in silico mutated residues	1669:1698	Endochitinase activity was restored in both proteins by mutating residues A117E (HbCLP1) and A147E (HbCLP2); the distance between the catalytic proton donor and the catalytic nucleophile in the in silico mutated residues was 9.5 Å, as occurs in inverting enzymes.
25104038	5	57	theme	activity	964:971	arg1	lack					956:959	the lack	952:959	the lack of activity	952:971	Sequence alignments showed that in the two proteins the catalytic residues Glu117 and Glu147 in HbCLP1 and HbCLP2, respectively, were mutated to Ala, accounting for the lack of activity.
25104038	4	58	theme	unusual	723:729	arg1	domain					751:756	an unusual half chitin-binding domain	720:756	an unusual half chitin-binding domain before the catalytic domain	720:784	HbCLP1 was identical to the allergen Hev b 11.0101 previously described by others, while HbCLP2 was a novel isoform exhibiting an unusual half chitin-binding domain before the catalytic domain.
25104038	10	59	theme	inverting	1724:1732	arg1	enzymes					1734:1740	inverting enzymes	1724:1740	inverting enzymes	1724:1740	Endochitinase activity was restored in both proteins by mutating residues A117E (HbCLP1) and A147E (HbCLP2); the distance between the catalytic proton donor and the catalytic nucleophile in the in silico mutated residues was 9.5 Å, as occurs in inverting enzymes.
25104038	10	60	theme	in	1673:1674	arg1	residues					1691:1698	the in silico mutated residues	1669:1698	the in silico mutated residues	1669:1698	Endochitinase activity was restored in both proteins by mutating residues A117E (HbCLP1) and A147E (HbCLP2); the distance between the catalytic proton donor and the catalytic nucleophile in the in silico mutated residues was 9.5 Å, as occurs in inverting enzymes.
25104038	6	61	theme	fluorescence	1122:1133	arg1	experiments					1135:1145	tryptophan fluorescence experiments	1111:1145	tryptophan fluorescence experiments	1111:1145	Nonetheless, both CLPs bound chitin and chitotriose (GlcNAc)3 with high affinities, as evaluated with chitin-affinity chromatography and tryptophan fluorescence experiments.
25104038	4	62	theme	allergen	621:628	arg1	11.0101					636:642	the allergen Hev b 11.0101	617:642	the allergen Hev b 11.0101 previously described by others	617:673	HbCLP1 was identical to the allergen Hev b 11.0101 previously described by others, while HbCLP2 was a novel isoform exhibiting an unusual half chitin-binding domain before the catalytic domain.
25104038	3	63	from	brasiliensis	518:529	arg1	CLPs					467:470	two CLPs	463:470	two CLPs of the GH19 family from the rubber tree Hevea brasiliensis (HbCLP1 and HbCLP2)	463:549	In this study, two CLPs of the GH19 family from the rubber tree Hevea brasiliensis (HbCLP1 and HbCLP2) were cloned, expressed and characterized.
25104038	9	64	theme	crystallographic	1337:1352	arg1	models					1354:1359	crystallographic models	1337:1359	crystallographic models	1337:1359	The analysis of the crystallographic models and docking experiments using (GlcNAc)6 oligosaccharides provides evidence of the residues involved in sugar binding.
25104038	7	65	theme	chitin-binding	1152:1165	arg1	domains					1167:1173	The chitin-binding domains	1148:1173	The chitin-binding domains	1148:1173	The chitin-binding domains also bound chitotriose with even higher affinities.
25104038	6	66	theme	chitotriose	1014:1024	arg1	GlcNAc					1027:1032	chitotriose (GlcNAc)3	1014:1034	chitotriose (GlcNAc)3	1014:1034	Nonetheless, both CLPs bound chitin and chitotriose (GlcNAc)3 with high affinities, as evaluated with chitin-affinity chromatography and tryptophan fluorescence experiments.
25104038	4	67	theme	chitin-binding	736:749	arg1	domain					751:756	an unusual half chitin-binding domain	720:756	an unusual half chitin-binding domain before the catalytic domain	720:784	HbCLP1 was identical to the allergen Hev b 11.0101 previously described by others, while HbCLP2 was a novel isoform exhibiting an unusual half chitin-binding domain before the catalytic domain.
25104038	4	68	theme	Hev	630:632	arg1	11.0101					636:642	the allergen Hev b 11.0101	617:642	the allergen Hev b 11.0101 previously described by others	617:673	HbCLP1 was identical to the allergen Hev b 11.0101 previously described by others, while HbCLP2 was a novel isoform exhibiting an unusual half chitin-binding domain before the catalytic domain.
25104038	1	69	theme	GH18	235:238	arg1	families					249:256	the GH18 and GH19 families	231:256	the GH18 and GH19 families	231:256	Plants express chitinase and chitinase-like proteins (CLPs) belonging to the glycosyl hydrolases of the GH18 and GH19 families, which exhibit varied functions.
25104038	0	70	theme	Hevea	59:63	arg1	brasiliensis					65:76	Hevea brasiliensis	59:76	Hevea brasiliensis	59:76	Comparative study of two GH19 chitinase-like proteins from Hevea brasiliensis, one exhibiting a novel carbohydrate-binding domain.
25104038	11	71	theme	plant	1884:1888	arg1	mechanisms					1898:1907	plant defense mechanisms	1884:1907	plant defense mechanisms	1884:1907	HbCLP1 and HbCLP2 were highly thermostable and exhibited antifungal activity against Alternaria alternata, suggesting their participation in plant defense mechanisms.
25104038	4	72	theme	half	731:734	arg1	domain					751:756	an unusual half chitin-binding domain	720:756	an unusual half chitin-binding domain before the catalytic domain	720:784	HbCLP1 was identical to the allergen Hev b 11.0101 previously described by others, while HbCLP2 was a novel isoform exhibiting an unusual half chitin-binding domain before the catalytic domain.
25104038	3	73	theme	GH19	479:482	arg1	family					484:489	the GH19 family	475:489	the GH19 family	475:489	In this study, two CLPs of the GH19 family from the rubber tree Hevea brasiliensis (HbCLP1 and HbCLP2) were cloned, expressed and characterized.
25104038	10	74	theme	catalytic	1644:1652	arg1	nucleophile					1654:1664	the catalytic nucleophile	1640:1664	the catalytic nucleophile	1640:1664	Endochitinase activity was restored in both proteins by mutating residues A117E (HbCLP1) and A147E (HbCLP2); the distance between the catalytic proton donor and the catalytic nucleophile in the in silico mutated residues was 9.5 Å, as occurs in inverting enzymes.
24901527	4	0	theme	1,4-linked	800:809	arg1	residues					840:847	1,4-linked α-D-galacturonic acid (GalA) residues	800:847	1,4-linked α-D-galacturonic acid (GalA) residues	800:847	A combination of composition, methylation and configuration analyses, as well as NMR (nuclear magnetic resonance) spectroscopy revealed that TPS1-2a and TPS1-2b were homogalacturonan (HG) pectins consisting of a backbone of 1,4-linked α-D-galacturonic acid (GalA) residues with 28.4% and 26.1% of carboxyl groups as methyl ester, respectively.
24901527	1	1	theme	20	146:147	arg1	kDa					149:151	kDa	149:151	kDa	149:151	Two natural homogalacturonan (HG) pectins (MW ca. 20 kDa) were isolated from green tea based on their immunomodulatory activity.
24901527	4	2	theme	methyl	892:897	arg1	ester					899:903	methyl ester	892:903	methyl ester	892:903	A combination of composition, methylation and configuration analyses, as well as NMR (nuclear magnetic resonance) spectroscopy revealed that TPS1-2a and TPS1-2b were homogalacturonan (HG) pectins consisting of a backbone of 1,4-linked α-D-galacturonic acid (GalA) residues with 28.4% and 26.1% of carboxyl groups as methyl ester, respectively.
24901527	5	3	theme	HL-60	1058:1062	arg1	cells					1064:1068	HL-60 cells	1058:1068	HL-60 cells	1058:1068	The immunological assay results demonstrated that TPS1-2, which consisted mainly of HG pectins, showed phagocytosis-enhancing activity in HL-60 cells.
24901527	2	4	theme	hot	310:312	arg1	extraction					320:329	hot water extraction	310:329	hot water extraction	310:329	The crude tea polysaccharides (TPS1 and TPS2) were obtained from green tea leaves by hot water extraction and followed by 40% and 70% ethanol precipitation, respectively.
24901527	4	5	theme	acid	828:831	arg1	residues					840:847	1,4-linked α-D-galacturonic acid (GalA) residues	800:847	1,4-linked α-D-galacturonic acid (GalA) residues	800:847	A combination of composition, methylation and configuration analyses, as well as NMR (nuclear magnetic resonance) spectroscopy revealed that TPS1-2a and TPS1-2b were homogalacturonan (HG) pectins consisting of a backbone of 1,4-linked α-D-galacturonic acid (GalA) residues with 28.4% and 26.1% of carboxyl groups as methyl ester, respectively.
24901527	1	6	dep	pectins	130:136	arg1	MW					139:140	MW	139:140	MW	139:140	Two natural homogalacturonan (HG) pectins (MW ca. 20 kDa) were isolated from green tea based on their immunomodulatory activity.
24901527	4	7	theme	magnetic	670:677	arg1	resonance					679:687	nuclear magnetic resonance	662:687	nuclear magnetic resonance	662:687	A combination of composition, methylation and configuration analyses, as well as NMR (nuclear magnetic resonance) spectroscopy revealed that TPS1-2a and TPS1-2b were homogalacturonan (HG) pectins consisting of a backbone of 1,4-linked α-D-galacturonic acid (GalA) residues with 28.4% and 26.1% of carboxyl groups as methyl ester, respectively.
24901527	4	7	theme	magnetic	670:677	arg1	NMR					657:659	NMR	657:659	NMR (nuclear magnetic resonance) spectroscopy	657:701	A combination of composition, methylation and configuration analyses, as well as NMR (nuclear magnetic resonance) spectroscopy revealed that TPS1-2a and TPS1-2b were homogalacturonan (HG) pectins consisting of a backbone of 1,4-linked α-D-galacturonic acid (GalA) residues with 28.4% and 26.1% of carboxyl groups as methyl ester, respectively.
24901527	4	8	theme	α-D-galacturonic	811:826	arg1	residues					840:847	1,4-linked α-D-galacturonic acid (GalA) residues	800:847	1,4-linked α-D-galacturonic acid (GalA) residues	800:847	A combination of composition, methylation and configuration analyses, as well as NMR (nuclear magnetic resonance) spectroscopy revealed that TPS1-2a and TPS1-2b were homogalacturonan (HG) pectins consisting of a backbone of 1,4-linked α-D-galacturonic acid (GalA) residues with 28.4% and 26.1% of carboxyl groups as methyl ester, respectively.
24901527	2	9	theme	water	314:318	arg1	extraction					320:329	hot water extraction	310:329	hot water extraction	310:329	The crude tea polysaccharides (TPS1 and TPS2) were obtained from green tea leaves by hot water extraction and followed by 40% and 70% ethanol precipitation, respectively.
24901527	4	10	theme	NMR	657:659	arg1	spectroscopy					690:701	NMR (nuclear magnetic resonance) spectroscopy	657:701	NMR (nuclear magnetic resonance) spectroscopy	657:701	A combination of composition, methylation and configuration analyses, as well as NMR (nuclear magnetic resonance) spectroscopy revealed that TPS1-2a and TPS1-2b were homogalacturonan (HG) pectins consisting of a backbone of 1,4-linked α-D-galacturonic acid (GalA) residues with 28.4% and 26.1% of carboxyl groups as methyl ester, respectively.
24901527	4	11	theme	carboxyl	873:880	arg1	groups					882:887	carboxyl groups	873:887	carboxyl groups as methyl ester	873:903	A combination of composition, methylation and configuration analyses, as well as NMR (nuclear magnetic resonance) spectroscopy revealed that TPS1-2a and TPS1-2b were homogalacturonan (HG) pectins consisting of a backbone of 1,4-linked α-D-galacturonic acid (GalA) residues with 28.4% and 26.1% of carboxyl groups as methyl ester, respectively.
24901527	4	12	theme	nuclear	662:668	arg1	resonance					679:687	nuclear magnetic resonance	662:687	nuclear magnetic resonance	662:687	A combination of composition, methylation and configuration analyses, as well as NMR (nuclear magnetic resonance) spectroscopy revealed that TPS1-2a and TPS1-2b were homogalacturonan (HG) pectins consisting of a backbone of 1,4-linked α-D-galacturonic acid (GalA) residues with 28.4% and 26.1% of carboxyl groups as methyl ester, respectively.
24901527	4	12	theme	nuclear	662:668	arg1	NMR					657:659	NMR	657:659	NMR (nuclear magnetic resonance) spectroscopy	657:701	A combination of composition, methylation and configuration analyses, as well as NMR (nuclear magnetic resonance) spectroscopy revealed that TPS1-2a and TPS1-2b were homogalacturonan (HG) pectins consisting of a backbone of 1,4-linked α-D-galacturonic acid (GalA) residues with 28.4% and 26.1% of carboxyl groups as methyl ester, respectively.
24901527	3	13	theme	soluble	417:423	arg1	TPS1-2a					442:448	TPS1-2a	442:448	TPS1-2a	442:448	Two homogenous water soluble polysaccharides (TPS1-2a and TPS1-2b) were obtained from TPS1 after purification with gel permeation, which gave a higher phagocytic effect than TPS2.
24901527	3	13	theme	soluble	417:423	arg1	polysaccharides					425:439	Two homogenous water soluble polysaccharides	396:439	Two homogenous water soluble polysaccharides (TPS1-2a and TPS1-2b)	396:461	Two homogenous water soluble polysaccharides (TPS1-2a and TPS1-2b) were obtained from TPS1 after purification with gel permeation, which gave a higher phagocytic effect than TPS2.
24901527	3	13	theme	soluble	417:423	arg1	TPS1-2b					454:460	TPS1-2b	454:460	TPS1-2b	454:460	Two homogenous water soluble polysaccharides (TPS1-2a and TPS1-2b) were obtained from TPS1 after purification with gel permeation, which gave a higher phagocytic effect than TPS2.
24901527	5	14	theme	HG	1004:1005	arg1	pectins					1007:1013	HG pectins	1004:1013	HG pectins	1004:1013	The immunological assay results demonstrated that TPS1-2, which consisted mainly of HG pectins, showed phagocytosis-enhancing activity in HL-60 cells.
24901527	3	15	with	purification	493:504	arg1	permeation					515:524	gel permeation	511:524	gel permeation	511:524	Two homogenous water soluble polysaccharides (TPS1-2a and TPS1-2b) were obtained from TPS1 after purification with gel permeation, which gave a higher phagocytic effect than TPS2.
24901527	4	16	theme	homogalacturonan	742:757	arg1	pectins					764:770	homogalacturonan (HG) pectins	742:770	homogalacturonan (HG) pectins consisting of a backbone of 1,4-linked α-D-galacturonic acid (GalA) residues	742:847	A combination of composition, methylation and configuration analyses, as well as NMR (nuclear magnetic resonance) spectroscopy revealed that TPS1-2a and TPS1-2b were homogalacturonan (HG) pectins consisting of a backbone of 1,4-linked α-D-galacturonic acid (GalA) residues with 28.4% and 26.1% of carboxyl groups as methyl ester, respectively.
24901527	4	17	theme	composition	593:603	arg1	combination					578:588	A combination	576:588	A combination of composition, methylation and configuration analyses, as well as NMR (nuclear magnetic resonance) spectroscopy	576:701	A combination of composition, methylation and configuration analyses, as well as NMR (nuclear magnetic resonance) spectroscopy revealed that TPS1-2a and TPS1-2b were homogalacturonan (HG) pectins consisting of a backbone of 1,4-linked α-D-galacturonic acid (GalA) residues with 28.4% and 26.1% of carboxyl groups as methyl ester, respectively.
24901527	5	18	theme	assay	938:942	arg1	results					944:950	The immunological assay results	920:950	The immunological assay results	920:950	The immunological assay results demonstrated that TPS1-2, which consisted mainly of HG pectins, showed phagocytosis-enhancing activity in HL-60 cells.
24901527	5	19	theme	phagocytosis-enhancing	1023:1044	arg1	activity					1046:1053	phagocytosis-enhancing activity	1023:1053	phagocytosis-enhancing activity in HL-60 cells	1023:1068	The immunological assay results demonstrated that TPS1-2, which consisted mainly of HG pectins, showed phagocytosis-enhancing activity in HL-60 cells.
24901527	2	20	dep	tea	296:298	arg1	leaves					300:305	leaves	300:305	leaves	300:305	The crude tea polysaccharides (TPS1 and TPS2) were obtained from green tea leaves by hot water extraction and followed by 40% and 70% ethanol precipitation, respectively.
24901527	4	21	link	1,4-linked	800:809	arg1	residues					840:847	1,4-linked α-D-galacturonic acid (GalA) residues	800:847	1,4-linked α-D-galacturonic acid (GalA) residues	800:847	A combination of composition, methylation and configuration analyses, as well as NMR (nuclear magnetic resonance) spectroscopy revealed that TPS1-2a and TPS1-2b were homogalacturonan (HG) pectins consisting of a backbone of 1,4-linked α-D-galacturonic acid (GalA) residues with 28.4% and 26.1% of carboxyl groups as methyl ester, respectively.
24901527	2	22	dep	polysaccharides	239:253	arg1	TPS2					265:268	TPS2	265:268	TPS2	265:268	The crude tea polysaccharides (TPS1 and TPS2) were obtained from green tea leaves by hot water extraction and followed by 40% and 70% ethanol precipitation, respectively.
24901527	2	22	dep	polysaccharides	239:253	arg1	TPS1					256:259	TPS1	256:259	TPS1	256:259	The crude tea polysaccharides (TPS1 and TPS2) were obtained from green tea leaves by hot water extraction and followed by 40% and 70% ethanol precipitation, respectively.
24901527	2	22	dep	polysaccharides	239:253	arg1	polysaccharides					239:253	The crude tea polysaccharides	225:253	The crude tea polysaccharides (TPS1 and TPS2)	225:269	The crude tea polysaccharides (TPS1 and TPS2) were obtained from green tea leaves by hot water extraction and followed by 40% and 70% ethanol precipitation, respectively.
24901527	1	23	theme	natural	100:106	arg1	pectins					130:136	Two natural homogalacturonan (HG) pectins	96:136	Two natural homogalacturonan (HG) pectins (MW ca. 20 kDa)	96:152	Two natural homogalacturonan (HG) pectins (MW ca. 20 kDa) were isolated from green tea based on their immunomodulatory activity.
24901527	4	24	theme	methylation	606:616	arg1	combination					578:588	A combination	576:588	A combination of composition, methylation and configuration analyses, as well as NMR (nuclear magnetic resonance) spectroscopy	576:701	A combination of composition, methylation and configuration analyses, as well as NMR (nuclear magnetic resonance) spectroscopy revealed that TPS1-2a and TPS1-2b were homogalacturonan (HG) pectins consisting of a backbone of 1,4-linked α-D-galacturonic acid (GalA) residues with 28.4% and 26.1% of carboxyl groups as methyl ester, respectively.
24901527	0	25	theme	homogalacturonan	24:39	arg1	pectins					41:47	two homogalacturonan pectins	20:47	two homogalacturonan pectins	20:47	Characterization of two homogalacturonan pectins with immunomodulatory activity from green tea.
24901527	3	26	theme	phagocytic	547:556	arg1	effect					558:563	a higher phagocytic effect	538:563	a higher phagocytic effect	538:563	Two homogenous water soluble polysaccharides (TPS1-2a and TPS1-2b) were obtained from TPS1 after purification with gel permeation, which gave a higher phagocytic effect than TPS2.
24901527	1	27	dep	MW	139:140	arg1	ca.					142:144	MW ca.	139:144	MW ca. 20 kDa	139:151	Two natural homogalacturonan (HG) pectins (MW ca. 20 kDa) were isolated from green tea based on their immunomodulatory activity.
24901527	2	28	theme	ethanol	359:365	arg1	precipitation					367:379	70% ethanol precipitation	355:379	70% ethanol precipitation	355:379	The crude tea polysaccharides (TPS1 and TPS2) were obtained from green tea leaves by hot water extraction and followed by 40% and 70% ethanol precipitation, respectively.
24901527	3	29	theme	water	411:415	arg1	TPS1-2a					442:448	TPS1-2a	442:448	TPS1-2a	442:448	Two homogenous water soluble polysaccharides (TPS1-2a and TPS1-2b) were obtained from TPS1 after purification with gel permeation, which gave a higher phagocytic effect than TPS2.
24901527	3	29	theme	water	411:415	arg1	polysaccharides					425:439	Two homogenous water soluble polysaccharides	396:439	Two homogenous water soluble polysaccharides (TPS1-2a and TPS1-2b)	396:461	Two homogenous water soluble polysaccharides (TPS1-2a and TPS1-2b) were obtained from TPS1 after purification with gel permeation, which gave a higher phagocytic effect than TPS2.
24901527	3	29	theme	water	411:415	arg1	TPS1-2b					454:460	TPS1-2b	454:460	TPS1-2b	454:460	Two homogenous water soluble polysaccharides (TPS1-2a and TPS1-2b) were obtained from TPS1 after purification with gel permeation, which gave a higher phagocytic effect than TPS2.
24901527	3	30	theme	gel	511:513	arg1	permeation					515:524	gel permeation	511:524	gel permeation	511:524	Two homogenous water soluble polysaccharides (TPS1-2a and TPS1-2b) were obtained from TPS1 after purification with gel permeation, which gave a higher phagocytic effect than TPS2.
24901527	2	31	theme	%	357:357	arg1	precipitation					367:379	70% ethanol precipitation	355:379	70% ethanol precipitation	355:379	The crude tea polysaccharides (TPS1 and TPS2) were obtained from green tea leaves by hot water extraction and followed by 40% and 70% ethanol precipitation, respectively.
24901527	0	32	from	tea	91:93	arg1	activity					71:78	immunomodulatory activity	54:78	immunomodulatory activity from green tea	54:93	Characterization of two homogalacturonan pectins with immunomodulatory activity from green tea.
24901527	5	33	theme	immunological	924:936	arg1	results					944:950	The immunological assay results	920:950	The immunological assay results	920:950	The immunological assay results demonstrated that TPS1-2, which consisted mainly of HG pectins, showed phagocytosis-enhancing activity in HL-60 cells.
24901527	2	34	theme	70	355:356	arg1	%					357:357	%	357:357	%	357:357	The crude tea polysaccharides (TPS1 and TPS2) were obtained from green tea leaves by hot water extraction and followed by 40% and 70% ethanol precipitation, respectively.
24901527	0	35	theme	pectins	41:47	arg1	Characterization					0:15	Characterization	0:15	Characterization of two homogalacturonan pectins with immunomodulatory activity from green tea.	0:94	Characterization of two homogalacturonan pectins with immunomodulatory activity from green tea.
24901527	3	36	theme	higher	540:545	arg1	effect					558:563	a higher phagocytic effect	538:563	a higher phagocytic effect	538:563	Two homogenous water soluble polysaccharides (TPS1-2a and TPS1-2b) were obtained from TPS1 after purification with gel permeation, which gave a higher phagocytic effect than TPS2.
24901527	3	37	theme	homogenous	400:409	arg1	TPS1-2a					442:448	TPS1-2a	442:448	TPS1-2a	442:448	Two homogenous water soluble polysaccharides (TPS1-2a and TPS1-2b) were obtained from TPS1 after purification with gel permeation, which gave a higher phagocytic effect than TPS2.
24901527	3	37	theme	homogenous	400:409	arg1	polysaccharides					425:439	Two homogenous water soluble polysaccharides	396:439	Two homogenous water soluble polysaccharides (TPS1-2a and TPS1-2b)	396:461	Two homogenous water soluble polysaccharides (TPS1-2a and TPS1-2b) were obtained from TPS1 after purification with gel permeation, which gave a higher phagocytic effect than TPS2.
24901527	3	37	theme	homogenous	400:409	arg1	TPS1-2b					454:460	TPS1-2b	454:460	TPS1-2b	454:460	Two homogenous water soluble polysaccharides (TPS1-2a and TPS1-2b) were obtained from TPS1 after purification with gel permeation, which gave a higher phagocytic effect than TPS2.
24901527	5	38	from	activity	1046:1053	arg1	cells					1064:1068	HL-60 cells	1058:1068	HL-60 cells	1058:1068	The immunological assay results demonstrated that TPS1-2, which consisted mainly of HG pectins, showed phagocytosis-enhancing activity in HL-60 cells.
24901527	4	39	theme	GalA	834:837	arg1	residues					840:847	1,4-linked α-D-galacturonic acid (GalA) residues	800:847	1,4-linked α-D-galacturonic acid (GalA) residues	800:847	A combination of composition, methylation and configuration analyses, as well as NMR (nuclear magnetic resonance) spectroscopy revealed that TPS1-2a and TPS1-2b were homogalacturonan (HG) pectins consisting of a backbone of 1,4-linked α-D-galacturonic acid (GalA) residues with 28.4% and 26.1% of carboxyl groups as methyl ester, respectively.
24901527	1	40	attach	isolated	159:166	arg1	tea					179:181	green tea	173:181	green tea based on their immunomodulatory activity	173:222	Two natural homogalacturonan (HG) pectins (MW ca. 20 kDa) were isolated from green tea based on their immunomodulatory activity.
24901527	1	40	attach	isolated	159:166	arg2	pectins					130:136	Two natural homogalacturonan (HG) pectins	96:136	Two natural homogalacturonan (HG) pectins (MW ca. 20 kDa)	96:152	Two natural homogalacturonan (HG) pectins (MW ca. 20 kDa) were isolated from green tea based on their immunomodulatory activity.
24901527	0	41	theme	immunomodulatory	54:69	arg1	activity					71:78	immunomodulatory activity	54:78	immunomodulatory activity from green tea	54:93	Characterization of two homogalacturonan pectins with immunomodulatory activity from green tea.
24901527	3	42	dep	polysaccharides	425:439	arg1	TPS1-2a					442:448	TPS1-2a	442:448	TPS1-2a	442:448	Two homogenous water soluble polysaccharides (TPS1-2a and TPS1-2b) were obtained from TPS1 after purification with gel permeation, which gave a higher phagocytic effect than TPS2.
24901527	3	42	dep	polysaccharides	425:439	arg1	polysaccharides					425:439	Two homogenous water soluble polysaccharides	396:439	Two homogenous water soluble polysaccharides (TPS1-2a and TPS1-2b)	396:461	Two homogenous water soluble polysaccharides (TPS1-2a and TPS1-2b) were obtained from TPS1 after purification with gel permeation, which gave a higher phagocytic effect than TPS2.
24901527	3	42	dep	polysaccharides	425:439	arg1	TPS1-2b					454:460	TPS1-2b	454:460	TPS1-2b	454:460	Two homogenous water soluble polysaccharides (TPS1-2a and TPS1-2b) were obtained from TPS1 after purification with gel permeation, which gave a higher phagocytic effect than TPS2.
24901527	4	43	theme	residues	840:847	arg1	backbone					788:795	a backbone	786:795	a backbone of 1,4-linked α-D-galacturonic acid (GalA) residues	786:847	A combination of composition, methylation and configuration analyses, as well as NMR (nuclear magnetic resonance) spectroscopy revealed that TPS1-2a and TPS1-2b were homogalacturonan (HG) pectins consisting of a backbone of 1,4-linked α-D-galacturonic acid (GalA) residues with 28.4% and 26.1% of carboxyl groups as methyl ester, respectively.
24901527	0	44	with	Characterization	0:15	arg1	activity					71:78	immunomodulatory activity	54:78	immunomodulatory activity from green tea	54:93	Characterization of two homogalacturonan pectins with immunomodulatory activity from green tea.
24901527	1	45	theme	homogalacturonan	108:123	arg1	pectins					130:136	Two natural homogalacturonan (HG) pectins	96:136	Two natural homogalacturonan (HG) pectins (MW ca. 20 kDa)	96:152	Two natural homogalacturonan (HG) pectins (MW ca. 20 kDa) were isolated from green tea based on their immunomodulatory activity.
24901527	4	46	with	pectins	764:770	arg1	%					858:858	28.4%	854:858	28.4%	854:858	A combination of composition, methylation and configuration analyses, as well as NMR (nuclear magnetic resonance) spectroscopy revealed that TPS1-2a and TPS1-2b were homogalacturonan (HG) pectins consisting of a backbone of 1,4-linked α-D-galacturonic acid (GalA) residues with 28.4% and 26.1% of carboxyl groups as methyl ester, respectively.
24901527	4	46	with	pectins	764:770	arg1	%					868:868	26.1%	864:868	26.1%	864:868	A combination of composition, methylation and configuration analyses, as well as NMR (nuclear magnetic resonance) spectroscopy revealed that TPS1-2a and TPS1-2b were homogalacturonan (HG) pectins consisting of a backbone of 1,4-linked α-D-galacturonic acid (GalA) residues with 28.4% and 26.1% of carboxyl groups as methyl ester, respectively.
24901527	4	46	with	pectins	764:770	arg1	groups					882:887	carboxyl groups	873:887	carboxyl groups as methyl ester	873:903	A combination of composition, methylation and configuration analyses, as well as NMR (nuclear magnetic resonance) spectroscopy revealed that TPS1-2a and TPS1-2b were homogalacturonan (HG) pectins consisting of a backbone of 1,4-linked α-D-galacturonic acid (GalA) residues with 28.4% and 26.1% of carboxyl groups as methyl ester, respectively.
24901527	2	47	theme	tea	235:237	arg1	TPS2					265:268	TPS2	265:268	TPS2	265:268	The crude tea polysaccharides (TPS1 and TPS2) were obtained from green tea leaves by hot water extraction and followed by 40% and 70% ethanol precipitation, respectively.
24901527	2	47	theme	tea	235:237	arg1	TPS1					256:259	TPS1	256:259	TPS1	256:259	The crude tea polysaccharides (TPS1 and TPS2) were obtained from green tea leaves by hot water extraction and followed by 40% and 70% ethanol precipitation, respectively.
24901527	2	47	theme	tea	235:237	arg1	polysaccharides					239:253	The crude tea polysaccharides	225:253	The crude tea polysaccharides (TPS1 and TPS2)	225:269	The crude tea polysaccharides (TPS1 and TPS2) were obtained from green tea leaves by hot water extraction and followed by 40% and 70% ethanol precipitation, respectively.
24901527	2	48	theme	green	290:294	arg1	tea					296:298	green tea leaves	290:305	green tea leaves	290:305	The crude tea polysaccharides (TPS1 and TPS2) were obtained from green tea leaves by hot water extraction and followed by 40% and 70% ethanol precipitation, respectively.
24901527	1	49	theme	immunomodulatory	198:213	arg1	activity					215:222	their immunomodulatory activity	192:222	their immunomodulatory activity	192:222	Two natural homogalacturonan (HG) pectins (MW ca. 20 kDa) were isolated from green tea based on their immunomodulatory activity.
24901527	2	50	theme	crude	229:233	arg1	TPS2					265:268	TPS2	265:268	TPS2	265:268	The crude tea polysaccharides (TPS1 and TPS2) were obtained from green tea leaves by hot water extraction and followed by 40% and 70% ethanol precipitation, respectively.
24901527	2	50	theme	crude	229:233	arg1	TPS1					256:259	TPS1	256:259	TPS1	256:259	The crude tea polysaccharides (TPS1 and TPS2) were obtained from green tea leaves by hot water extraction and followed by 40% and 70% ethanol precipitation, respectively.
24901527	2	50	theme	crude	229:233	arg1	polysaccharides					239:253	The crude tea polysaccharides	225:253	The crude tea polysaccharides (TPS1 and TPS2)	225:269	The crude tea polysaccharides (TPS1 and TPS2) were obtained from green tea leaves by hot water extraction and followed by 40% and 70% ethanol precipitation, respectively.
24901527	1	51	theme	green	173:177	arg1	tea					179:181	green tea	173:181	green tea based on their immunomodulatory activity	173:222	Two natural homogalacturonan (HG) pectins (MW ca. 20 kDa) were isolated from green tea based on their immunomodulatory activity.
24901527	4	52	theme	analyses	636:643	arg1	combination					578:588	A combination	576:588	A combination of composition, methylation and configuration analyses, as well as NMR (nuclear magnetic resonance) spectroscopy	576:701	A combination of composition, methylation and configuration analyses, as well as NMR (nuclear magnetic resonance) spectroscopy revealed that TPS1-2a and TPS1-2b were homogalacturonan (HG) pectins consisting of a backbone of 1,4-linked α-D-galacturonic acid (GalA) residues with 28.4% and 26.1% of carboxyl groups as methyl ester, respectively.
24901527	4	53	theme	groups	882:887	arg1	%					858:858	28.4%	854:858	28.4%	854:858	A combination of composition, methylation and configuration analyses, as well as NMR (nuclear magnetic resonance) spectroscopy revealed that TPS1-2a and TPS1-2b were homogalacturonan (HG) pectins consisting of a backbone of 1,4-linked α-D-galacturonic acid (GalA) residues with 28.4% and 26.1% of carboxyl groups as methyl ester, respectively.
24901527	4	53	theme	groups	882:887	arg1	%					868:868	26.1%	864:868	26.1%	864:868	A combination of composition, methylation and configuration analyses, as well as NMR (nuclear magnetic resonance) spectroscopy revealed that TPS1-2a and TPS1-2b were homogalacturonan (HG) pectins consisting of a backbone of 1,4-linked α-D-galacturonic acid (GalA) residues with 28.4% and 26.1% of carboxyl groups as methyl ester, respectively.
24901527	4	53	theme	groups	882:887	arg1	groups					882:887	carboxyl groups	873:887	carboxyl groups as methyl ester	873:903	A combination of composition, methylation and configuration analyses, as well as NMR (nuclear magnetic resonance) spectroscopy revealed that TPS1-2a and TPS1-2b were homogalacturonan (HG) pectins consisting of a backbone of 1,4-linked α-D-galacturonic acid (GalA) residues with 28.4% and 26.1% of carboxyl groups as methyl ester, respectively.
24901527	4	54	theme	configuration	622:634	arg1	analyses					636:643	configuration analyses	622:643	configuration analyses	622:643	A combination of composition, methylation and configuration analyses, as well as NMR (nuclear magnetic resonance) spectroscopy revealed that TPS1-2a and TPS1-2b were homogalacturonan (HG) pectins consisting of a backbone of 1,4-linked α-D-galacturonic acid (GalA) residues with 28.4% and 26.1% of carboxyl groups as methyl ester, respectively.
27283664	1	0	from	composition	159:169	arg1	varieties					235:243	four common bean varieties	218:243	four common bean varieties	218:243	The impact of extrusion cooking on the chemical composition and functional properties of bean powders from four common bean varieties was investigated.
27283664	5	1	theme	non-extruded	897:908	arg1	powders					935:941	the cooked non-extruded and cooked extruded bean powders	886:941	the cooked non-extruded and cooked extruded bean powders	886:941	The starch digestibilities of the cooked non-extruded and cooked extruded bean powders were comparable.
27283664	3	2	theme	stachyose	652:660	arg1	contents					662:669	the sucrose, raffinose and stachyose contents	625:669	the sucrose, raffinose and stachyose contents	625:669	Compared with corresponding non-extruded (raw) bean powders (particle size⩽0.5mm), the extrusion treatments did not substantially change the protein and starch contents of the bean powders and showed inconsistent effects on the sucrose, raffinose and stachyose contents.
27283664	4	3	theme	bean	767:770	arg1	powders					772:778	the bean powders	763:778	the bean powders	763:778	The extrusion cooking did cause complete starch gelatinization and protein denaturation of the bean powders and thus changed their pasting properties and solvent-retention capacities.
27283664	6	4	theme	functional	996:1005	arg1	properties					1007:1016	functional properties	996:1016	functional properties similar to those of two commercial bean powders	996:1064	The extruded bean powders displayed functional properties similar to those of two commercial bean powders.
27283664	6	5	theme	similar	1018:1024	arg1	properties					1007:1016	functional properties	996:1016	functional properties similar to those of two commercial bean powders	996:1064	The extruded bean powders displayed functional properties similar to those of two commercial bean powders.
27283664	0	6	theme	common	90:95	arg1	powders					102:108	dry common bean powders	86:108	dry common bean powders	86:108	Effects of extrusion cooking on the chemical composition and functional properties of dry common bean powders.
27283664	0	7	from	Effects	0:6	arg1	properties					72:81	functional properties	61:81	functional properties	61:81	Effects of extrusion cooking on the chemical composition and functional properties of dry common bean powders.
27283664	0	7	from	Effects	0:6	arg1	composition					45:55	chemical composition	36:55	chemical composition	36:55	Effects of extrusion cooking on the chemical composition and functional properties of dry common bean powders.
27283664	5	8	theme	cooked	890:895	arg1	powders					935:941	the cooked non-extruded and cooked extruded bean powders	886:941	the cooked non-extruded and cooked extruded bean powders	886:941	The starch digestibilities of the cooked non-extruded and cooked extruded bean powders were comparable.
27283664	0	9	theme	dry	86:88	arg1	powders					102:108	dry common bean powders	86:108	dry common bean powders	86:108	Effects of extrusion cooking on the chemical composition and functional properties of dry common bean powders.
27283664	4	10	theme	protein	739:745	arg1	denaturation					747:758	protein denaturation	739:758	protein denaturation	739:758	The extrusion cooking did cause complete starch gelatinization and protein denaturation of the bean powders and thus changed their pasting properties and solvent-retention capacities.
27283664	2	11	theme	bean	271:274	arg1	powders					276:282	The raw bean powders	263:282	The raw bean powders	263:282	The raw bean powders were extruded under eight different conditions, and the extrudates were then dried and ground (particle size⩽0.5mm).
27283664	5	12	theme	powders	935:941	arg1	digestibilities					867:881	The starch digestibilities	856:881	The starch digestibilities of the cooked non-extruded and cooked extruded bean powders	856:941	The starch digestibilities of the cooked non-extruded and cooked extruded bean powders were comparable.
27283664	5	12	theme	powders	935:941	arg1	comparable					948:957	comparable	948:957	comparable	948:957	The starch digestibilities of the cooked non-extruded and cooked extruded bean powders were comparable.
27283664	0	13	theme	powders	102:108	arg1	properties					72:81	functional properties	61:81	functional properties	61:81	Effects of extrusion cooking on the chemical composition and functional properties of dry common bean powders.
27283664	0	13	theme	powders	102:108	arg1	composition					45:55	chemical composition	36:55	chemical composition	36:55	Effects of extrusion cooking on the chemical composition and functional properties of dry common bean powders.
27283664	5	14	theme	cooked	914:919	arg1	powders					935:941	the cooked non-extruded and cooked extruded bean powders	886:941	the cooked non-extruded and cooked extruded bean powders	886:941	The starch digestibilities of the cooked non-extruded and cooked extruded bean powders were comparable.
27283664	4	15	theme	solvent-retention	826:842	arg1	capacities					844:853	solvent-retention capacities	826:853	solvent-retention capacities	826:853	The extrusion cooking did cause complete starch gelatinization and protein denaturation of the bean powders and thus changed their pasting properties and solvent-retention capacities.
27283664	4	16	theme	starch	713:718	arg1	gelatinization					720:733	complete starch gelatinization	704:733	complete starch gelatinization	704:733	The extrusion cooking did cause complete starch gelatinization and protein denaturation of the bean powders and thus changed their pasting properties and solvent-retention capacities.
27283664	2	17	theme	raw	267:269	arg1	powders					276:282	The raw bean powders	263:282	The raw bean powders	263:282	The raw bean powders were extruded under eight different conditions, and the extrudates were then dried and ground (particle size⩽0.5mm).
27283664	1	18	theme	chemical	150:157	arg1	composition					159:169	chemical composition	150:169	chemical composition	150:169	The impact of extrusion cooking on the chemical composition and functional properties of bean powders from four common bean varieties was investigated.
27283664	0	19	theme	bean	97:100	arg1	powders					102:108	dry common bean powders	86:108	dry common bean powders	86:108	Effects of extrusion cooking on the chemical composition and functional properties of dry common bean powders.
27283664	1	20	from	impact	115:120	arg1	composition					159:169	chemical composition	150:169	chemical composition	150:169	The impact of extrusion cooking on the chemical composition and functional properties of bean powders from four common bean varieties was investigated.
27283664	1	20	from	impact	115:120	arg1	properties					186:195	functional properties	175:195	functional properties	175:195	The impact of extrusion cooking on the chemical composition and functional properties of bean powders from four common bean varieties was investigated.
27283664	6	21	theme	extruded	964:971	arg1	powders					978:984	The extruded bean powders	960:984	The extruded bean powders	960:984	The extruded bean powders displayed functional properties similar to those of two commercial bean powders.
27283664	3	22	theme	non-extruded	429:440	arg1	powders					453:459	corresponding non-extruded (raw) bean powders	415:459	corresponding non-extruded (raw) bean powders (particle size⩽0.5mm)	415:481	Compared with corresponding non-extruded (raw) bean powders (particle size⩽0.5mm), the extrusion treatments did not substantially change the protein and starch contents of the bean powders and showed inconsistent effects on the sucrose, raffinose and stachyose contents.
27283664	3	22	theme	non-extruded	429:440	arg1	size⩽0.5mm					471:480	particle size⩽0.5mm	462:480	particle size⩽0.5mm	462:480	Compared with corresponding non-extruded (raw) bean powders (particle size⩽0.5mm), the extrusion treatments did not substantially change the protein and starch contents of the bean powders and showed inconsistent effects on the sucrose, raffinose and stachyose contents.
27283664	3	23	theme	extrusion	488:496	arg1	treatments					498:507	the extrusion treatments	484:507	the extrusion treatments	484:507	Compared with corresponding non-extruded (raw) bean powders (particle size⩽0.5mm), the extrusion treatments did not substantially change the protein and starch contents of the bean powders and showed inconsistent effects on the sucrose, raffinose and stachyose contents.
27283664	3	24	theme	protein	542:548	arg1	contents					561:568	the protein and starch contents	538:568	contents	561:568	Compared with corresponding non-extruded (raw) bean powders (particle size⩽0.5mm), the extrusion treatments did not substantially change the protein and starch contents of the bean powders and showed inconsistent effects on the sucrose, raffinose and stachyose contents.
27283664	4	25	theme	complete	704:711	arg1	gelatinization					720:733	complete starch gelatinization	704:733	complete starch gelatinization	704:733	The extrusion cooking did cause complete starch gelatinization and protein denaturation of the bean powders and thus changed their pasting properties and solvent-retention capacities.
27283664	1	26	theme	functional	175:184	arg1	properties					186:195	functional properties	175:195	functional properties	175:195	The impact of extrusion cooking on the chemical composition and functional properties of bean powders from four common bean varieties was investigated.
27283664	0	27	theme	cooking	21:27	arg1	Effects					0:6	Effects	0:6	Effects of extrusion cooking on the chemical composition and functional properties of dry common bean powders	0:108	Effects of extrusion cooking on the chemical composition and functional properties of dry common bean powders.
27283664	4	28	theme	pasting	803:809	arg1	properties					811:820	their pasting properties	797:820	their pasting properties	797:820	The extrusion cooking did cause complete starch gelatinization and protein denaturation of the bean powders and thus changed their pasting properties and solvent-retention capacities.
27283664	0	29	theme	extrusion	11:19	arg1	cooking					21:27	extrusion cooking	11:27	extrusion cooking	11:27	Effects of extrusion cooking on the chemical composition and functional properties of dry common bean powders.
27283664	6	30	theme	bean	973:976	arg1	powders					978:984	The extruded bean powders	960:984	The extruded bean powders	960:984	The extruded bean powders displayed functional properties similar to those of two commercial bean powders.
27283664	3	31	theme	bean	448:451	arg1	powders					453:459	corresponding non-extruded (raw) bean powders	415:459	corresponding non-extruded (raw) bean powders (particle size⩽0.5mm)	415:481	Compared with corresponding non-extruded (raw) bean powders (particle size⩽0.5mm), the extrusion treatments did not substantially change the protein and starch contents of the bean powders and showed inconsistent effects on the sucrose, raffinose and stachyose contents.
27283664	3	31	theme	bean	448:451	arg1	size⩽0.5mm					471:480	particle size⩽0.5mm	462:480	particle size⩽0.5mm	462:480	Compared with corresponding non-extruded (raw) bean powders (particle size⩽0.5mm), the extrusion treatments did not substantially change the protein and starch contents of the bean powders and showed inconsistent effects on the sucrose, raffinose and stachyose contents.
27283664	5	32	theme	extruded	921:928	arg1	powders					935:941	the cooked non-extruded and cooked extruded bean powders	886:941	the cooked non-extruded and cooked extruded bean powders	886:941	The starch digestibilities of the cooked non-extruded and cooked extruded bean powders were comparable.
27283664	1	33	from	varieties	235:243	arg1	composition					159:169	chemical composition	150:169	chemical composition	150:169	The impact of extrusion cooking on the chemical composition and functional properties of bean powders from four common bean varieties was investigated.
27283664	1	33	from	varieties	235:243	arg1	properties					186:195	functional properties	175:195	functional properties	175:195	The impact of extrusion cooking on the chemical composition and functional properties of bean powders from four common bean varieties was investigated.
27283664	1	33	from	varieties	235:243	arg1	powders					205:211	bean powders	200:211	bean powders from four common bean varieties	200:243	The impact of extrusion cooking on the chemical composition and functional properties of bean powders from four common bean varieties was investigated.
27283664	0	34	dep	composition	45:55	arg1	the					32:34	the	32:34	the	32:34	Effects of extrusion cooking on the chemical composition and functional properties of dry common bean powders.
27283664	1	35	theme	bean	200:203	arg1	powders					205:211	bean powders	200:211	bean powders from four common bean varieties	200:243	The impact of extrusion cooking on the chemical composition and functional properties of bean powders from four common bean varieties was investigated.
27283664	3	36	theme	corresponding	415:427	arg1	powders					453:459	corresponding non-extruded (raw) bean powders	415:459	corresponding non-extruded (raw) bean powders (particle size⩽0.5mm)	415:481	Compared with corresponding non-extruded (raw) bean powders (particle size⩽0.5mm), the extrusion treatments did not substantially change the protein and starch contents of the bean powders and showed inconsistent effects on the sucrose, raffinose and stachyose contents.
27283664	3	36	theme	corresponding	415:427	arg1	size⩽0.5mm					471:480	particle size⩽0.5mm	462:480	particle size⩽0.5mm	462:480	Compared with corresponding non-extruded (raw) bean powders (particle size⩽0.5mm), the extrusion treatments did not substantially change the protein and starch contents of the bean powders and showed inconsistent effects on the sucrose, raffinose and stachyose contents.
27283664	3	37	theme	inconsistent	601:612	arg1	effects					614:620	inconsistent effects	601:620	inconsistent effects on the sucrose, raffinose and stachyose contents	601:669	Compared with corresponding non-extruded (raw) bean powders (particle size⩽0.5mm), the extrusion treatments did not substantially change the protein and starch contents of the bean powders and showed inconsistent effects on the sucrose, raffinose and stachyose contents.
27283664	1	38	theme	powders	205:211	arg1	composition					159:169	chemical composition	150:169	chemical composition	150:169	The impact of extrusion cooking on the chemical composition and functional properties of bean powders from four common bean varieties was investigated.
27283664	1	38	theme	powders	205:211	arg1	properties					186:195	functional properties	175:195	functional properties	175:195	The impact of extrusion cooking on the chemical composition and functional properties of bean powders from four common bean varieties was investigated.
27283664	6	39	theme	bean	1053:1056	arg1	powders					1058:1064	two commercial bean powders	1038:1064	two commercial bean powders	1038:1064	The extruded bean powders displayed functional properties similar to those of two commercial bean powders.
27283664	3	40	theme	starch	554:559	arg1	contents					561:568	the protein and starch contents	538:568	contents	561:568	Compared with corresponding non-extruded (raw) bean powders (particle size⩽0.5mm), the extrusion treatments did not substantially change the protein and starch contents of the bean powders and showed inconsistent effects on the sucrose, raffinose and stachyose contents.
27283664	3	41	theme	raw	443:445	arg1	powders					453:459	corresponding non-extruded (raw) bean powders	415:459	corresponding non-extruded (raw) bean powders (particle size⩽0.5mm)	415:481	Compared with corresponding non-extruded (raw) bean powders (particle size⩽0.5mm), the extrusion treatments did not substantially change the protein and starch contents of the bean powders and showed inconsistent effects on the sucrose, raffinose and stachyose contents.
27283664	3	41	theme	raw	443:445	arg1	size⩽0.5mm					471:480	particle size⩽0.5mm	462:480	particle size⩽0.5mm	462:480	Compared with corresponding non-extruded (raw) bean powders (particle size⩽0.5mm), the extrusion treatments did not substantially change the protein and starch contents of the bean powders and showed inconsistent effects on the sucrose, raffinose and stachyose contents.
27283664	3	42	theme	particle	462:469	arg1	powders					453:459	corresponding non-extruded (raw) bean powders	415:459	corresponding non-extruded (raw) bean powders (particle size⩽0.5mm)	415:481	Compared with corresponding non-extruded (raw) bean powders (particle size⩽0.5mm), the extrusion treatments did not substantially change the protein and starch contents of the bean powders and showed inconsistent effects on the sucrose, raffinose and stachyose contents.
27283664	3	42	theme	particle	462:469	arg1	size⩽0.5mm					471:480	particle size⩽0.5mm	462:480	particle size⩽0.5mm	462:480	Compared with corresponding non-extruded (raw) bean powders (particle size⩽0.5mm), the extrusion treatments did not substantially change the protein and starch contents of the bean powders and showed inconsistent effects on the sucrose, raffinose and stachyose contents.
27283664	0	43	theme	chemical	36:43	arg1	composition					45:55	chemical composition	36:55	chemical composition	36:55	Effects of extrusion cooking on the chemical composition and functional properties of dry common bean powders.
27283664	3	44	theme	raffinose	638:646	arg1	contents					662:669	the sucrose, raffinose and stachyose contents	625:669	the sucrose, raffinose and stachyose contents	625:669	Compared with corresponding non-extruded (raw) bean powders (particle size⩽0.5mm), the extrusion treatments did not substantially change the protein and starch contents of the bean powders and showed inconsistent effects on the sucrose, raffinose and stachyose contents.
27283664	1	45	dep	composition	159:169	arg1	the					146:148	the	146:148	the	146:148	The impact of extrusion cooking on the chemical composition and functional properties of bean powders from four common bean varieties was investigated.
27283664	1	46	from	properties	186:195	arg1	varieties					235:243	four common bean varieties	218:243	four common bean varieties	218:243	The impact of extrusion cooking on the chemical composition and functional properties of bean powders from four common bean varieties was investigated.
27283664	4	47	theme	extrusion	676:684	arg1	cooking					686:692	The extrusion cooking	672:692	The extrusion cooking	672:692	The extrusion cooking did cause complete starch gelatinization and protein denaturation of the bean powders and thus changed their pasting properties and solvent-retention capacities.
27283664	4	48	theme	powders	772:778	arg1	gelatinization					720:733	complete starch gelatinization	704:733	complete starch gelatinization	704:733	The extrusion cooking did cause complete starch gelatinization and protein denaturation of the bean powders and thus changed their pasting properties and solvent-retention capacities.
27283664	4	48	theme	powders	772:778	arg1	denaturation					747:758	protein denaturation	739:758	protein denaturation	739:758	The extrusion cooking did cause complete starch gelatinization and protein denaturation of the bean powders and thus changed their pasting properties and solvent-retention capacities.
27283664	5	49	theme	bean	930:933	arg1	powders					935:941	the cooked non-extruded and cooked extruded bean powders	886:941	the cooked non-extruded and cooked extruded bean powders	886:941	The starch digestibilities of the cooked non-extruded and cooked extruded bean powders were comparable.
27283664	6	50	theme	commercial	1042:1051	arg1	powders					1058:1064	two commercial bean powders	1038:1064	two commercial bean powders	1038:1064	The extruded bean powders displayed functional properties similar to those of two commercial bean powders.
27283664	3	51	theme	sucrose	629:635	arg1	contents					662:669	the sucrose, raffinose and stachyose contents	625:669	the sucrose, raffinose and stachyose contents	625:669	Compared with corresponding non-extruded (raw) bean powders (particle size⩽0.5mm), the extrusion treatments did not substantially change the protein and starch contents of the bean powders and showed inconsistent effects on the sucrose, raffinose and stachyose contents.
27283664	2	52	dep	dried	361:365	arg1	size⩽0.5mm					388:397	particle size⩽0.5mm	379:397	particle size⩽0.5mm	379:397	The raw bean powders were extruded under eight different conditions, and the extrudates were then dried and ground (particle size⩽0.5mm).
27283664	2	52	dep	dried	361:365	arg1	dried					361:365	dried	361:365	dried	361:365	The raw bean powders were extruded under eight different conditions, and the extrudates were then dried and ground (particle size⩽0.5mm).
27283664	2	52	dep	dried	361:365	arg1	extrudates					340:349	the extrudates	336:349	the extrudates	336:349	The raw bean powders were extruded under eight different conditions, and the extrudates were then dried and ground (particle size⩽0.5mm).
27283664	2	53	theme	different	310:318	arg1	conditions					320:329	eight different conditions	304:329	eight different conditions	304:329	The raw bean powders were extruded under eight different conditions, and the extrudates were then dried and ground (particle size⩽0.5mm).
27283664	0	54	theme	functional	61:70	arg1	properties					72:81	functional properties	61:81	functional properties	61:81	Effects of extrusion cooking on the chemical composition and functional properties of dry common bean powders.
27283664	2	55	theme	particle	379:386	arg1	size⩽0.5mm					388:397	particle size⩽0.5mm	379:397	particle size⩽0.5mm	379:397	The raw bean powders were extruded under eight different conditions, and the extrudates were then dried and ground (particle size⩽0.5mm).
27283664	2	55	theme	particle	379:386	arg1	dried					361:365	dried	361:365	dried	361:365	The raw bean powders were extruded under eight different conditions, and the extrudates were then dried and ground (particle size⩽0.5mm).
27283664	2	55	theme	particle	379:386	arg1	extrudates					340:349	the extrudates	336:349	the extrudates	336:349	The raw bean powders were extruded under eight different conditions, and the extrudates were then dried and ground (particle size⩽0.5mm).
27283664	3	56	theme	bean	577:580	arg1	powders					582:588	the bean powders	573:588	the bean powders	573:588	Compared with corresponding non-extruded (raw) bean powders (particle size⩽0.5mm), the extrusion treatments did not substantially change the protein and starch contents of the bean powders and showed inconsistent effects on the sucrose, raffinose and stachyose contents.
27283664	1	57	theme	extrusion	125:133	arg1	cooking					135:141	extrusion cooking	125:141	extrusion cooking	125:141	The impact of extrusion cooking on the chemical composition and functional properties of bean powders from four common bean varieties was investigated.
27283664	5	58	theme	starch	860:865	arg1	digestibilities					867:881	The starch digestibilities	856:881	The starch digestibilities of the cooked non-extruded and cooked extruded bean powders	856:941	The starch digestibilities of the cooked non-extruded and cooked extruded bean powders were comparable.
27283664	5	58	theme	starch	860:865	arg1	comparable					948:957	comparable	948:957	comparable	948:957	The starch digestibilities of the cooked non-extruded and cooked extruded bean powders were comparable.
27283664	1	59	theme	common	223:228	arg1	varieties					235:243	four common bean varieties	218:243	four common bean varieties	218:243	The impact of extrusion cooking on the chemical composition and functional properties of bean powders from four common bean varieties was investigated.
27283664	3	60	theme	powders	582:588	arg1	contents					561:568	the protein and starch contents	538:568	contents	561:568	Compared with corresponding non-extruded (raw) bean powders (particle size⩽0.5mm), the extrusion treatments did not substantially change the protein and starch contents of the bean powders and showed inconsistent effects on the sucrose, raffinose and stachyose contents.
27283664	3	61	from	effects	614:620	arg1	contents					662:669	the sucrose, raffinose and stachyose contents	625:669	the sucrose, raffinose and stachyose contents	625:669	Compared with corresponding non-extruded (raw) bean powders (particle size⩽0.5mm), the extrusion treatments did not substantially change the protein and starch contents of the bean powders and showed inconsistent effects on the sucrose, raffinose and stachyose contents.
27283664	1	62	theme	cooking	135:141	arg1	impact					115:120	The impact	111:120	The impact of extrusion cooking on the chemical composition and functional properties of bean powders from four common bean varieties	111:243	The impact of extrusion cooking on the chemical composition and functional properties of bean powders from four common bean varieties was investigated.
27283664	1	63	theme	bean	230:233	arg1	varieties					235:243	four common bean varieties	218:243	four common bean varieties	218:243	The impact of extrusion cooking on the chemical composition and functional properties of bean powders from four common bean varieties was investigated.
24557922	5	0	theme	wall-related	1073:1084	arg1	machinery					1086:1094	cell wall-related machinery	1068:1094	cell wall-related machinery	1068:1094	The complex cell wall architecture is controlled and organized in part by the dynamic intracellular cytoskeleton and by diverse trafficking pathways of the cell wall polymers and cell wall-related machinery.
24557922	5	1	theme	polymers	1055:1062	arg1	pathways					1029:1036	diverse trafficking pathways	1009:1036	diverse trafficking pathways of the cell wall polymers and cell wall-related machinery	1009:1094	The complex cell wall architecture is controlled and organized in part by the dynamic intracellular cytoskeleton and by diverse trafficking pathways of the cell wall polymers and cell wall-related machinery.
24557922	7	2	theme	essential	1326:1334	arg1	structure					1336:1344	the intricate plant cell wall--an essential structure	1292:1344	the intricate plant cell wall--an essential structure	1292:1344	These many processes cooperate to construct, maintain, and manipulate the intricate plant cell wall--an essential structure for the sustaining of the plant stature, growth, and life.
24557922	0	3	from	wall	5:8	arg1	plants					54:59	higher plants	47:59	higher plants	47:59	Cell wall, cytoskeleton, and cell expansion in higher plants.
24557922	5	4	theme	trafficking	1017:1027	arg1	pathways					1029:1036	diverse trafficking pathways	1009:1036	diverse trafficking pathways of the cell wall polymers and cell wall-related machinery	1009:1094	The complex cell wall architecture is controlled and organized in part by the dynamic intracellular cytoskeleton and by diverse trafficking pathways of the cell wall polymers and cell wall-related machinery.
24557922	1	5	theme	protein	403:409	arg1	network					333:339	an intricate network	320:339	an intricate network of cellulosic, hemicellulosic, and pectic polysaccharides and protein	320:409	To accommodate two seemingly contradictory biological roles in plant physiology, providing both the rigid structural support of plant cells and the adjustable elasticity needed for cell expansion, the composition of the plant cell wall has evolved to become an intricate network of cellulosic, hemicellulosic, and pectic polysaccharides and protein.
24557922	5	6	theme	wall	1050:1053	arg1	polymers					1055:1062	the cell wall polymers	1041:1062	the cell wall polymers	1041:1062	The complex cell wall architecture is controlled and organized in part by the dynamic intracellular cytoskeleton and by diverse trafficking pathways of the cell wall polymers and cell wall-related machinery.
24557922	3	7	theme	polymers	602:609	arg1	roles					619:623	the roles	615:623	the roles of the variety of proteins involved in polysaccharide synthesis	615:687	The biosynthesis of cell wall polymers and the roles of the variety of proteins involved in polysaccharide synthesis continue to be characterized.
24557922	3	7	theme	polymers	602:609	arg1	biosynthesis					576:587	The biosynthesis	572:587	The biosynthesis of cell wall polymers	572:609	The biosynthesis of cell wall polymers and the roles of the variety of proteins involved in polysaccharide synthesis continue to be characterized.
24557922	2	8	theme	cell	481:484	arg1	expansion					486:494	plant cell expansion	475:494	plant cell expansion	475:494	Due to its complexity, many aspects of the cell wall influence plant cell expansion, and many new and insightful observations and technologies are forthcoming.
24557922	6	9	theme	sensing	1174:1180	arg1	stimuli					1182:1188	hormonal and integrity sensing stimuli	1151:1188	hormonal and integrity sensing stimuli that are perceived by the cell	1151:1219	Meanwhile, the cell wall is continually influenced by hormonal and integrity sensing stimuli that are perceived by the cell.
24557922	4	10	theme	plant	845:849	arg1	wall					856:859	the plant cell wall	841:859	the plant cell wall	841:859	The interactions within the cell wall polymer network and the modification of these interactions provide insight into how the plant cell wall provides its dual function.
24557922	6	11	theme	hormonal	1151:1158	arg1	stimuli					1182:1188	hormonal and integrity sensing stimuli	1151:1188	hormonal and integrity sensing stimuli that are perceived by the cell	1151:1219	Meanwhile, the cell wall is continually influenced by hormonal and integrity sensing stimuli that are perceived by the cell.
24557922	1	12	theme	cell	243:246	arg1	expansion					248:256	cell expansion	243:256	cell expansion	243:256	To accommodate two seemingly contradictory biological roles in plant physiology, providing both the rigid structural support of plant cells and the adjustable elasticity needed for cell expansion, the composition of the plant cell wall has evolved to become an intricate network of cellulosic, hemicellulosic, and pectic polysaccharides and protein.
24557922	2	13	theme	plant	475:479	arg1	expansion					486:494	plant cell expansion	475:494	plant cell expansion	475:494	Due to its complexity, many aspects of the cell wall influence plant cell expansion, and many new and insightful observations and technologies are forthcoming.
24557922	0	14	from	expansion	34:42	arg1	plants					54:59	higher plants	47:59	higher plants	47:59	Cell wall, cytoskeleton, and cell expansion in higher plants.
24557922	7	15	theme	plant	1372:1376	arg1	stature					1378:1384	the plant stature	1368:1384	the plant stature	1368:1384	These many processes cooperate to construct, maintain, and manipulate the intricate plant cell wall--an essential structure for the sustaining of the plant stature, growth, and life.
24557922	1	16	theme	intricate	323:331	arg1	network					333:339	an intricate network	320:339	an intricate network of cellulosic, hemicellulosic, and pectic polysaccharides and protein	320:409	To accommodate two seemingly contradictory biological roles in plant physiology, providing both the rigid structural support of plant cells and the adjustable elasticity needed for cell expansion, the composition of the plant cell wall has evolved to become an intricate network of cellulosic, hemicellulosic, and pectic polysaccharides and protein.
24557922	4	17	theme	interactions	803:814	arg1	network					765:771	the cell wall polymer network	743:771	the cell wall polymer network	743:771	The interactions within the cell wall polymer network and the modification of these interactions provide insight into how the plant cell wall provides its dual function.
24557922	4	17	theme	interactions	803:814	arg1	modification					781:792	the modification	777:792	the modification of these interactions	777:814	The interactions within the cell wall polymer network and the modification of these interactions provide insight into how the plant cell wall provides its dual function.
24557922	2	18	theme	wall	460:463	arg1	aspects					440:446	many aspects	435:446	many aspects of the cell wall	435:463	Due to its complexity, many aspects of the cell wall influence plant cell expansion, and many new and insightful observations and technologies are forthcoming.
24557922	5	19	theme	cell	1068:1071	arg1	machinery					1086:1094	cell wall-related machinery	1068:1094	cell wall-related machinery	1068:1094	The complex cell wall architecture is controlled and organized in part by the dynamic intracellular cytoskeleton and by diverse trafficking pathways of the cell wall polymers and cell wall-related machinery.
24557922	5	20	theme	machinery	1086:1094	arg1	pathways					1029:1036	diverse trafficking pathways	1009:1036	diverse trafficking pathways of the cell wall polymers and cell wall-related machinery	1009:1094	The complex cell wall architecture is controlled and organized in part by the dynamic intracellular cytoskeleton and by diverse trafficking pathways of the cell wall polymers and cell wall-related machinery.
24557922	7	21	theme	intricate	1296:1304	arg1	structure					1336:1344	the intricate plant cell wall--an essential structure	1292:1344	the intricate plant cell wall--an essential structure	1292:1344	These many processes cooperate to construct, maintain, and manipulate the intricate plant cell wall--an essential structure for the sustaining of the plant stature, growth, and life.
24557922	5	22	theme	cell	1045:1048	arg1	polymers					1055:1062	the cell wall polymers	1041:1062	the cell wall polymers	1041:1062	The complex cell wall architecture is controlled and organized in part by the dynamic intracellular cytoskeleton and by diverse trafficking pathways of the cell wall polymers and cell wall-related machinery.
24557922	3	23	theme	polysaccharide	664:677	arg1	synthesis					679:687	polysaccharide synthesis	664:687	polysaccharide synthesis	664:687	The biosynthesis of cell wall polymers and the roles of the variety of proteins involved in polysaccharide synthesis continue to be characterized.
24557922	2	24	theme	cell	455:458	arg1	wall					460:463	the cell wall	451:463	the cell wall	451:463	Due to its complexity, many aspects of the cell wall influence plant cell expansion, and many new and insightful observations and technologies are forthcoming.
24557922	5	25	theme	complex	893:899	arg1	architecture					911:922	The complex cell wall architecture	889:922	The complex cell wall architecture	889:922	The complex cell wall architecture is controlled and organized in part by the dynamic intracellular cytoskeleton and by diverse trafficking pathways of the cell wall polymers and cell wall-related machinery.
24557922	4	26	theme	cell	851:854	arg1	wall					856:859	the plant cell wall	841:859	the plant cell wall	841:859	The interactions within the cell wall polymer network and the modification of these interactions provide insight into how the plant cell wall provides its dual function.
24557922	1	27	theme	rigid	162:166	arg1	support					179:185	the rigid structural support	158:185	the rigid structural support of plant cells	158:200	To accommodate two seemingly contradictory biological roles in plant physiology, providing both the rigid structural support of plant cells and the adjustable elasticity needed for cell expansion, the composition of the plant cell wall has evolved to become an intricate network of cellulosic, hemicellulosic, and pectic polysaccharides and protein.
24557922	0	28	theme	Cell	0:3	arg1	wall					5:8	Cell wall	0:8	Cell wall	0:8	Cell wall, cytoskeleton, and cell expansion in higher plants.
24557922	5	29	theme	cell	901:904	arg1	architecture					911:922	The complex cell wall architecture	889:922	The complex cell wall architecture	889:922	The complex cell wall architecture is controlled and organized in part by the dynamic intracellular cytoskeleton and by diverse trafficking pathways of the cell wall polymers and cell wall-related machinery.
24557922	1	30	theme	structural	168:177	arg1	support					179:185	the rigid structural support	158:185	the rigid structural support of plant cells	158:200	To accommodate two seemingly contradictory biological roles in plant physiology, providing both the rigid structural support of plant cells and the adjustable elasticity needed for cell expansion, the composition of the plant cell wall has evolved to become an intricate network of cellulosic, hemicellulosic, and pectic polysaccharides and protein.
24557922	2	31	theme	many	501:504	arg1	observations					525:536	many new and insightful observations	501:536	many new and insightful observations	501:536	Due to its complexity, many aspects of the cell wall influence plant cell expansion, and many new and insightful observations and technologies are forthcoming.
24557922	7	32	theme	cell	1312:1315	arg1	structure					1336:1344	the intricate plant cell wall--an essential structure	1292:1344	the intricate plant cell wall--an essential structure	1292:1344	These many processes cooperate to construct, maintain, and manipulate the intricate plant cell wall--an essential structure for the sustaining of the plant stature, growth, and life.
24557922	5	33	theme	wall	906:909	arg1	architecture					911:922	The complex cell wall architecture	889:922	The complex cell wall architecture	889:922	The complex cell wall architecture is controlled and organized in part by the dynamic intracellular cytoskeleton and by diverse trafficking pathways of the cell wall polymers and cell wall-related machinery.
24557922	3	34	theme	proteins	643:650	arg1	proteins					643:650	proteins	643:650	proteins involved in polysaccharide synthesis	643:687	The biosynthesis of cell wall polymers and the roles of the variety of proteins involved in polysaccharide synthesis continue to be characterized.
24557922	3	34	theme	proteins	643:650	arg1	variety					632:638	the variety	628:638	the variety of proteins involved in polysaccharide synthesis	628:687	The biosynthesis of cell wall polymers and the roles of the variety of proteins involved in polysaccharide synthesis continue to be characterized.
24557922	4	35	theme	dual	874:877	arg1	function					879:886	its dual function	870:886	its dual function	870:886	The interactions within the cell wall polymer network and the modification of these interactions provide insight into how the plant cell wall provides its dual function.
24557922	1	36	theme	cellulosic	344:353	arg1	polysaccharides					383:397	cellulosic, hemicellulosic, and pectic polysaccharides	344:397	cellulosic, hemicellulosic, and pectic polysaccharides	344:397	To accommodate two seemingly contradictory biological roles in plant physiology, providing both the rigid structural support of plant cells and the adjustable elasticity needed for cell expansion, the composition of the plant cell wall has evolved to become an intricate network of cellulosic, hemicellulosic, and pectic polysaccharides and protein.
24557922	3	37	theme	cell	592:595	arg1	polymers					602:609	cell wall polymers	592:609	cell wall polymers	592:609	The biosynthesis of cell wall polymers and the roles of the variety of proteins involved in polysaccharide synthesis continue to be characterized.
24557922	2	38	theme	many	435:438	arg1	aspects					440:446	many aspects	435:446	many aspects of the cell wall	435:463	Due to its complexity, many aspects of the cell wall influence plant cell expansion, and many new and insightful observations and technologies are forthcoming.
24557922	5	39	theme	dynamic	967:973	arg1	cytoskeleton					989:1000	the dynamic intracellular cytoskeleton	963:1000	the dynamic intracellular cytoskeleton	963:1000	The complex cell wall architecture is controlled and organized in part by the dynamic intracellular cytoskeleton and by diverse trafficking pathways of the cell wall polymers and cell wall-related machinery.
24557922	1	40	theme	plant	190:194	arg1	cells					196:200	plant cells	190:200	plant cells	190:200	To accommodate two seemingly contradictory biological roles in plant physiology, providing both the rigid structural support of plant cells and the adjustable elasticity needed for cell expansion, the composition of the plant cell wall has evolved to become an intricate network of cellulosic, hemicellulosic, and pectic polysaccharides and protein.
24557922	1	41	theme	hemicellulosic	356:369	arg1	polysaccharides					383:397	cellulosic, hemicellulosic, and pectic polysaccharides	344:397	cellulosic, hemicellulosic, and pectic polysaccharides	344:397	To accommodate two seemingly contradictory biological roles in plant physiology, providing both the rigid structural support of plant cells and the adjustable elasticity needed for cell expansion, the composition of the plant cell wall has evolved to become an intricate network of cellulosic, hemicellulosic, and pectic polysaccharides and protein.
24557922	5	42	theme	intracellular	975:987	arg1	cytoskeleton					989:1000	the dynamic intracellular cytoskeleton	963:1000	the dynamic intracellular cytoskeleton	963:1000	The complex cell wall architecture is controlled and organized in part by the dynamic intracellular cytoskeleton and by diverse trafficking pathways of the cell wall polymers and cell wall-related machinery.
24557922	1	43	theme	cells	196:200	arg1	elasticity					221:230	the adjustable elasticity	206:230	the adjustable elasticity needed for cell expansion	206:256	To accommodate two seemingly contradictory biological roles in plant physiology, providing both the rigid structural support of plant cells and the adjustable elasticity needed for cell expansion, the composition of the plant cell wall has evolved to become an intricate network of cellulosic, hemicellulosic, and pectic polysaccharides and protein.
24557922	1	43	theme	cells	196:200	arg1	support					179:185	the rigid structural support	158:185	the rigid structural support of plant cells	158:200	To accommodate two seemingly contradictory biological roles in plant physiology, providing both the rigid structural support of plant cells and the adjustable elasticity needed for cell expansion, the composition of the plant cell wall has evolved to become an intricate network of cellulosic, hemicellulosic, and pectic polysaccharides and protein.
24557922	1	44	theme	plant	282:286	arg1	wall					293:296	the plant cell wall	278:296	the plant cell wall	278:296	To accommodate two seemingly contradictory biological roles in plant physiology, providing both the rigid structural support of plant cells and the adjustable elasticity needed for cell expansion, the composition of the plant cell wall has evolved to become an intricate network of cellulosic, hemicellulosic, and pectic polysaccharides and protein.
24557922	7	45	theme	wall--an	1317:1324	arg1	structure					1336:1344	the intricate plant cell wall--an essential structure	1292:1344	the intricate plant cell wall--an essential structure	1292:1344	These many processes cooperate to construct, maintain, and manipulate the intricate plant cell wall--an essential structure for the sustaining of the plant stature, growth, and life.
24557922	3	46	theme	wall	597:600	arg1	polymers					602:609	cell wall polymers	592:609	cell wall polymers	592:609	The biosynthesis of cell wall polymers and the roles of the variety of proteins involved in polysaccharide synthesis continue to be characterized.
24557922	1	47	theme	contradictory	91:103	arg1	roles					116:120	two seemingly contradictory biological roles	77:120	two seemingly contradictory biological roles	77:120	To accommodate two seemingly contradictory biological roles in plant physiology, providing both the rigid structural support of plant cells and the adjustable elasticity needed for cell expansion, the composition of the plant cell wall has evolved to become an intricate network of cellulosic, hemicellulosic, and pectic polysaccharides and protein.
24557922	0	48	theme	cell	29:32	arg1	expansion					34:42	cell expansion	29:42	cell expansion	29:42	Cell wall, cytoskeleton, and cell expansion in higher plants.
24557922	1	49	theme	cell	288:291	arg1	wall					293:296	the plant cell wall	278:296	the plant cell wall	278:296	To accommodate two seemingly contradictory biological roles in plant physiology, providing both the rigid structural support of plant cells and the adjustable elasticity needed for cell expansion, the composition of the plant cell wall has evolved to become an intricate network of cellulosic, hemicellulosic, and pectic polysaccharides and protein.
24557922	2	50	theme	insightful	514:523	arg1	observations					525:536	many new and insightful observations	501:536	many new and insightful observations	501:536	Due to its complexity, many aspects of the cell wall influence plant cell expansion, and many new and insightful observations and technologies are forthcoming.
24557922	5	51	theme	diverse	1009:1015	arg1	pathways					1029:1036	diverse trafficking pathways	1009:1036	diverse trafficking pathways of the cell wall polymers and cell wall-related machinery	1009:1094	The complex cell wall architecture is controlled and organized in part by the dynamic intracellular cytoskeleton and by diverse trafficking pathways of the cell wall polymers and cell wall-related machinery.
24557922	1	52	theme	biological	105:114	arg1	roles					116:120	two seemingly contradictory biological roles	77:120	two seemingly contradictory biological roles	77:120	To accommodate two seemingly contradictory biological roles in plant physiology, providing both the rigid structural support of plant cells and the adjustable elasticity needed for cell expansion, the composition of the plant cell wall has evolved to become an intricate network of cellulosic, hemicellulosic, and pectic polysaccharides and protein.
24557922	1	53	theme	wall	293:296	arg1	composition					263:273	the composition	259:273	the composition of the plant cell wall	259:296	To accommodate two seemingly contradictory biological roles in plant physiology, providing both the rigid structural support of plant cells and the adjustable elasticity needed for cell expansion, the composition of the plant cell wall has evolved to become an intricate network of cellulosic, hemicellulosic, and pectic polysaccharides and protein.
24557922	3	54	theme	variety	632:638	arg1	roles					619:623	the roles	615:623	the roles of the variety of proteins involved in polysaccharide synthesis	615:687	The biosynthesis of cell wall polymers and the roles of the variety of proteins involved in polysaccharide synthesis continue to be characterized.
24557922	3	54	theme	variety	632:638	arg1	biosynthesis					576:587	The biosynthesis	572:587	The biosynthesis of cell wall polymers	572:609	The biosynthesis of cell wall polymers and the roles of the variety of proteins involved in polysaccharide synthesis continue to be characterized.
24557922	1	55	theme	pectic	376:381	arg1	polysaccharides					383:397	cellulosic, hemicellulosic, and pectic polysaccharides	344:397	cellulosic, hemicellulosic, and pectic polysaccharides	344:397	To accommodate two seemingly contradictory biological roles in plant physiology, providing both the rigid structural support of plant cells and the adjustable elasticity needed for cell expansion, the composition of the plant cell wall has evolved to become an intricate network of cellulosic, hemicellulosic, and pectic polysaccharides and protein.
24557922	4	56	theme	wall	752:755	arg1	network					765:771	the cell wall polymer network	743:771	the cell wall polymer network	743:771	The interactions within the cell wall polymer network and the modification of these interactions provide insight into how the plant cell wall provides its dual function.
24557922	7	57	theme	many	1228:1231	arg1	processes					1233:1241	These many processes	1222:1241	These many processes	1222:1241	These many processes cooperate to construct, maintain, and manipulate the intricate plant cell wall--an essential structure for the sustaining of the plant stature, growth, and life.
24557922	6	58	theme	cell	1112:1115	arg1	wall					1117:1120	the cell wall	1108:1120	the cell wall	1108:1120	Meanwhile, the cell wall is continually influenced by hormonal and integrity sensing stimuli that are perceived by the cell.
24557922	6	58	theme	cell	1112:1115	arg1	Meanwhile					1097:1105	Meanwhile	1097:1105	Meanwhile	1097:1105	Meanwhile, the cell wall is continually influenced by hormonal and integrity sensing stimuli that are perceived by the cell.
24557922	2	59	theme	new	506:508	arg1	observations					525:536	many new and insightful observations	501:536	many new and insightful observations	501:536	Due to its complexity, many aspects of the cell wall influence plant cell expansion, and many new and insightful observations and technologies are forthcoming.
24557922	1	60	theme	polysaccharides	383:397	arg1	network					333:339	an intricate network	320:339	an intricate network of cellulosic, hemicellulosic, and pectic polysaccharides and protein	320:409	To accommodate two seemingly contradictory biological roles in plant physiology, providing both the rigid structural support of plant cells and the adjustable elasticity needed for cell expansion, the composition of the plant cell wall has evolved to become an intricate network of cellulosic, hemicellulosic, and pectic polysaccharides and protein.
24557922	4	61	theme	cell	747:750	arg1	network					765:771	the cell wall polymer network	743:771	the cell wall polymer network	743:771	The interactions within the cell wall polymer network and the modification of these interactions provide insight into how the plant cell wall provides its dual function.
24557922	0	62	theme	higher	47:52	arg1	plants					54:59	higher plants	47:59	higher plants	47:59	Cell wall, cytoskeleton, and cell expansion in higher plants.
24557922	7	63	theme	plant	1306:1310	arg1	structure					1336:1344	the intricate plant cell wall--an essential structure	1292:1344	the intricate plant cell wall--an essential structure	1292:1344	These many processes cooperate to construct, maintain, and manipulate the intricate plant cell wall--an essential structure for the sustaining of the plant stature, growth, and life.
24557922	1	64	theme	plant	125:129	arg1	physiology					131:140	plant physiology	125:140	plant physiology	125:140	To accommodate two seemingly contradictory biological roles in plant physiology, providing both the rigid structural support of plant cells and the adjustable elasticity needed for cell expansion, the composition of the plant cell wall has evolved to become an intricate network of cellulosic, hemicellulosic, and pectic polysaccharides and protein.
24557922	1	65	theme	adjustable	210:219	arg1	elasticity					221:230	the adjustable elasticity	206:230	the adjustable elasticity needed for cell expansion	206:256	To accommodate two seemingly contradictory biological roles in plant physiology, providing both the rigid structural support of plant cells and the adjustable elasticity needed for cell expansion, the composition of the plant cell wall has evolved to become an intricate network of cellulosic, hemicellulosic, and pectic polysaccharides and protein.
24557922	0	66	from	cytoskeleton	11:22	arg1	plants					54:59	higher plants	47:59	higher plants	47:59	Cell wall, cytoskeleton, and cell expansion in higher plants.
24557922	4	67	theme	polymer	757:763	arg1	network					765:771	the cell wall polymer network	743:771	the cell wall polymer network	743:771	The interactions within the cell wall polymer network and the modification of these interactions provide insight into how the plant cell wall provides its dual function.
28578968	2	0	theme	FTIR	329:332	arg1	spectroscopy					334:345	FTIR spectroscopy	329:345	FTIR spectroscopy	329:345	GC-MS, FTIR spectroscopy, methylation analysis and FE-SEM analysis were performed to elucidate the physicochemical characterization of these two fractions, and their in vitro antitumour activities were also evaluated.
28578968	4	1	theme	mol	795:797	arg1	%					782:782	29.1%	778:782	29.1%	778:782	Monosaccharide composition analysis revealed that LW1 and LW2 were mainly composed of galactose (52.4% and 57.4%, mol%) and glucose (29.1% and 22.2%, mol%), respectively.
28578968	4	1	theme	mol	795:797	arg1	%					798:798	mol%	795:798	mol%	795:798	Monosaccharide composition analysis revealed that LW1 and LW2 were mainly composed of galactose (52.4% and 57.4%, mol%) and glucose (29.1% and 22.2%, mol%), respectively.
28578968	5	2	theme	main	1204:1207	arg1	chain					1209:1213	the main chain	1200:1213	the main chain	1200:1213	Methylation results showed that the main chain of LW1 likely involves (1→4)-linked Galp and (1→4)-linked Glcp with its side chains being (1→4,6)-linked Galp through the O-6 position connected to the backbone, whereas the main chain of LW2 likely involves (1→4)-linked Galp and (1→4)-linked Glcp with its side chains being (1→3)-Galp through the O-6 position of (1→3,6)-Galp linked to the main chain.
28578968	3	3	theme	molecular	544:552	arg1	Mws					563:565	Mws	563:565	Mws	563:565	The molecular weights (Mws) of LW1 and LW2 as determined by HPGPC were 25.10 and 12.34kDa, respectively.
28578968	3	3	theme	molecular	544:552	arg1	25.10					611:615	25.10	611:615	25.10	611:615	The molecular weights (Mws) of LW1 and LW2 as determined by HPGPC were 25.10 and 12.34kDa, respectively.
28578968	3	3	theme	molecular	544:552	arg1	weights					554:560	The molecular weights	540:560	The molecular weights (Mws) of LW1 and LW2 as determined by HPGPC	540:604	The molecular weights (Mws) of LW1 and LW2 as determined by HPGPC were 25.10 and 12.34kDa, respectively.
28578968	6	4	theme	EPS	1300:1302	arg1	fractions					1304:1312	the two EPS fractions	1292:1312	the two EPS fractions	1292:1312	Evaluation of the microcosmic morphology, as revealed by FE-SEM analysis of the two EPS fractions, showed a sheet-like appearance with a folded surface and a compact structure.
28578968	1	5	theme	yak	266:268	arg1	milk					270:273	yak milk	266:273	yak milk obtained from the Gansu Tibetan area of China	266:319	Two high molecular weight fractions (LW1 and LW2) of exopolysaccharides (EPSs) produced by Lactobacillus casei SB27 were isolated from yak milk obtained from the Gansu Tibetan area of China.
28578968	7	6	theme	cells	1541:1545	arg1	proliferation					1500:1512	the proliferation	1496:1512	the proliferation of HT-29 colorectal cancer cells	1496:1545	The results from in vitro antitumour tests indicated that both LW1 and LW2 could significantly inhibit the proliferation of HT-29 colorectal cancer cells and up-regulated the expressions of Bad, Bax, Caspase-3 and -8 genes.
28578968	9	7	theme	antitumour	1855:1864	arg1	drugs					1866:1870	natural antitumour drugs	1847:1870	natural antitumour drugs	1847:1870	Our results suggested that LW1 and LW2 possess potential not only for use in functional food products but also as a source of natural antitumour drugs.
28578968	5	8	theme	1→3,6	1178:1182	arg1	-Galp					1184:1188	(1→3,6)-Galp	1177:1188	(1→3,6)-Galp linked to the main chain	1177:1213	Methylation results showed that the main chain of LW1 likely involves (1→4)-linked Galp and (1→4)-linked Glcp with its side chains being (1→4,6)-linked Galp through the O-6 position connected to the backbone, whereas the main chain of LW2 likely involves (1→4)-linked Galp and (1→4)-linked Glcp with its side chains being (1→3)-Galp through the O-6 position of (1→3,6)-Galp linked to the main chain.
28578968	7	9	theme	colorectal	1523:1532	arg1	cells					1541:1545	HT-29 colorectal cancer cells	1517:1545	HT-29 colorectal cancer cells	1517:1545	The results from in vitro antitumour tests indicated that both LW1 and LW2 could significantly inhibit the proliferation of HT-29 colorectal cancer cells and up-regulated the expressions of Bad, Bax, Caspase-3 and -8 genes.
28578968	4	10	theme	Monosaccharide	645:658	arg1	analysis					672:679	Monosaccharide composition analysis	645:679	Monosaccharide composition analysis	645:679	Monosaccharide composition analysis revealed that LW1 and LW2 were mainly composed of galactose (52.4% and 57.4%, mol%) and glucose (29.1% and 22.2%, mol%), respectively.
28578968	8	11	theme	TEM	1626:1628	arg1	images					1630:1635	TEM images	1626:1635	TEM images	1626:1635	Finally, TEM images revealed the apoptotic morphological changes of HT-29 cells induced by LW1 and LW2.
28578968	5	12	theme	-Galp	1184:1188	arg1	position					1165:1172	the O-6 position	1157:1172	the O-6 position of (1→3,6)-Galp linked to the main chain	1157:1213	Methylation results showed that the main chain of LW1 likely involves (1→4)-linked Galp and (1→4)-linked Glcp with its side chains being (1→4,6)-linked Galp through the O-6 position connected to the backbone, whereas the main chain of LW2 likely involves (1→4)-linked Galp and (1→4)-linked Glcp with its side chains being (1→3)-Galp through the O-6 position of (1→3,6)-Galp linked to the main chain.
28578968	1	13	theme	exopolysaccharides	184:201	arg1	LW2					176:178	LW2	176:178	LW2	176:178	Two high molecular weight fractions (LW1 and LW2) of exopolysaccharides (EPSs) produced by Lactobacillus casei SB27 were isolated from yak milk obtained from the Gansu Tibetan area of China.
28578968	1	13	theme	exopolysaccharides	184:201	arg1	LW1					168:170	LW1	168:170	LW1	168:170	Two high molecular weight fractions (LW1 and LW2) of exopolysaccharides (EPSs) produced by Lactobacillus casei SB27 were isolated from yak milk obtained from the Gansu Tibetan area of China.
28578968	1	13	theme	exopolysaccharides	184:201	arg1	fractions					157:165	Two high molecular weight fractions	131:165	Two high molecular weight fractions (LW1 and LW2) of exopolysaccharides (EPSs) produced by Lactobacillus casei SB27	131:245	Two high molecular weight fractions (LW1 and LW2) of exopolysaccharides (EPSs) produced by Lactobacillus casei SB27 were isolated from yak milk obtained from the Gansu Tibetan area of China.
28578968	1	14	theme	high	135:138	arg1	weight					150:155	high molecular weight	135:155	Two high molecular weight fractions (LW1 and LW2) of exopolysaccharides (EPSs) produced by Lactobacillus casei SB27	131:245	Two high molecular weight fractions (LW1 and LW2) of exopolysaccharides (EPSs) produced by Lactobacillus casei SB27 were isolated from yak milk obtained from the Gansu Tibetan area of China.
28578968	7	15	dep	Bad	1583:1585	arg1	genes					1610:1614	genes	1610:1614	genes	1610:1614	The results from in vitro antitumour tests indicated that both LW1 and LW2 could significantly inhibit the proliferation of HT-29 colorectal cancer cells and up-regulated the expressions of Bad, Bax, Caspase-3 and -8 genes.
28578968	8	16	theme	apoptotic	1650:1658	arg1	changes					1674:1680	the apoptotic morphological changes	1646:1680	the apoptotic morphological changes of HT-29 cells induced by LW1 and LW2	1646:1718	Finally, TEM images revealed the apoptotic morphological changes of HT-29 cells induced by LW1 and LW2.
28578968	3	17	theme	LW2	579:581	arg1	Mws					563:565	Mws	563:565	Mws	563:565	The molecular weights (Mws) of LW1 and LW2 as determined by HPGPC were 25.10 and 12.34kDa, respectively.
28578968	3	17	theme	LW2	579:581	arg1	25.10					611:615	25.10	611:615	25.10	611:615	The molecular weights (Mws) of LW1 and LW2 as determined by HPGPC were 25.10 and 12.34kDa, respectively.
28578968	3	17	theme	LW2	579:581	arg1	weights					554:560	The molecular weights	540:560	The molecular weights (Mws) of LW1 and LW2 as determined by HPGPC	540:604	The molecular weights (Mws) of LW1 and LW2 as determined by HPGPC were 25.10 and 12.34kDa, respectively.
28578968	5	18	link	-linked	1076:1082	arg1	Galp					1084:1087	(1→4)-linked Galp	1071:1087	(1→4)-linked Galp	1071:1087	Methylation results showed that the main chain of LW1 likely involves (1→4)-linked Galp and (1→4)-linked Glcp with its side chains being (1→4,6)-linked Galp through the O-6 position connected to the backbone, whereas the main chain of LW2 likely involves (1→4)-linked Galp and (1→4)-linked Glcp with its side chains being (1→3)-Galp through the O-6 position of (1→3,6)-Galp linked to the main chain.
28578968	3	19	theme	LW1	571:573	arg1	Mws					563:565	Mws	563:565	Mws	563:565	The molecular weights (Mws) of LW1 and LW2 as determined by HPGPC were 25.10 and 12.34kDa, respectively.
28578968	3	19	theme	LW1	571:573	arg1	25.10					611:615	25.10	611:615	25.10	611:615	The molecular weights (Mws) of LW1 and LW2 as determined by HPGPC were 25.10 and 12.34kDa, respectively.
28578968	3	19	theme	LW1	571:573	arg1	weights					554:560	The molecular weights	540:560	The molecular weights (Mws) of LW1 and LW2 as determined by HPGPC	540:604	The molecular weights (Mws) of LW1 and LW2 as determined by HPGPC were 25.10 and 12.34kDa, respectively.
28578968	5	20	theme	Methylation	816:826	arg1	results					828:834	Methylation results	816:834	Methylation results	816:834	Methylation results showed that the main chain of LW1 likely involves (1→4)-linked Galp and (1→4)-linked Glcp with its side chains being (1→4,6)-linked Galp through the O-6 position connected to the backbone, whereas the main chain of LW2 likely involves (1→4)-linked Galp and (1→4)-linked Glcp with its side chains being (1→3)-Galp through the O-6 position of (1→3,6)-Galp linked to the main chain.
28578968	5	21	link	-linked	1098:1104	arg1	Glcp					1106:1109	(1→4)-linked Glcp	1093:1109	(1→4)-linked Glcp	1093:1109	Methylation results showed that the main chain of LW1 likely involves (1→4)-linked Galp and (1→4)-linked Glcp with its side chains being (1→4,6)-linked Galp through the O-6 position connected to the backbone, whereas the main chain of LW2 likely involves (1→4)-linked Galp and (1→4)-linked Glcp with its side chains being (1→3)-Galp through the O-6 position of (1→3,6)-Galp linked to the main chain.
28578968	5	22	theme	1→4	1094:1096	arg1	Glcp					1106:1109	(1→4)-linked Glcp	1093:1109	(1→4)-linked Glcp	1093:1109	Methylation results showed that the main chain of LW1 likely involves (1→4)-linked Galp and (1→4)-linked Glcp with its side chains being (1→4,6)-linked Galp through the O-6 position connected to the backbone, whereas the main chain of LW2 likely involves (1→4)-linked Galp and (1→4)-linked Glcp with its side chains being (1→3)-Galp through the O-6 position of (1→3,6)-Galp linked to the main chain.
28578968	8	23	theme	HT-29	1685:1689	arg1	cells					1691:1695	HT-29 cells	1685:1695	HT-29 cells	1685:1695	Finally, TEM images revealed the apoptotic morphological changes of HT-29 cells induced by LW1 and LW2.
28578968	1	24	theme	weight	150:155	arg1	LW2					176:178	LW2	176:178	LW2	176:178	Two high molecular weight fractions (LW1 and LW2) of exopolysaccharides (EPSs) produced by Lactobacillus casei SB27 were isolated from yak milk obtained from the Gansu Tibetan area of China.
28578968	1	24	theme	weight	150:155	arg1	LW1					168:170	LW1	168:170	LW1	168:170	Two high molecular weight fractions (LW1 and LW2) of exopolysaccharides (EPSs) produced by Lactobacillus casei SB27 were isolated from yak milk obtained from the Gansu Tibetan area of China.
28578968	1	24	theme	weight	150:155	arg1	fractions					157:165	Two high molecular weight fractions	131:165	Two high molecular weight fractions (LW1 and LW2) of exopolysaccharides (EPSs) produced by Lactobacillus casei SB27	131:245	Two high molecular weight fractions (LW1 and LW2) of exopolysaccharides (EPSs) produced by Lactobacillus casei SB27 were isolated from yak milk obtained from the Gansu Tibetan area of China.
28578968	5	25	theme	-linked	1098:1104	arg1	Glcp					1106:1109	(1→4)-linked Glcp	1093:1109	(1→4)-linked Glcp	1093:1109	Methylation results showed that the main chain of LW1 likely involves (1→4)-linked Galp and (1→4)-linked Glcp with its side chains being (1→4,6)-linked Galp through the O-6 position connected to the backbone, whereas the main chain of LW2 likely involves (1→4)-linked Galp and (1→4)-linked Glcp with its side chains being (1→3)-Galp through the O-6 position of (1→3,6)-Galp linked to the main chain.
28578968	1	26	theme	China	315:319	arg1	area					307:310	the Gansu Tibetan area	289:310	the Gansu Tibetan area of China	289:319	Two high molecular weight fractions (LW1 and LW2) of exopolysaccharides (EPSs) produced by Lactobacillus casei SB27 were isolated from yak milk obtained from the Gansu Tibetan area of China.
28578968	5	27	theme	-linked	1076:1082	arg1	Galp					1084:1087	(1→4)-linked Galp	1071:1087	(1→4)-linked Galp	1071:1087	Methylation results showed that the main chain of LW1 likely involves (1→4)-linked Galp and (1→4)-linked Glcp with its side chains being (1→4,6)-linked Galp through the O-6 position connected to the backbone, whereas the main chain of LW2 likely involves (1→4)-linked Galp and (1→4)-linked Glcp with its side chains being (1→3)-Galp through the O-6 position of (1→3,6)-Galp linked to the main chain.
28578968	9	28	contain	possess	1760:1766	arg1	LW1					1748:1750	LW1	1748:1750	LW1	1748:1750	Our results suggested that LW1 and LW2 possess potential not only for use in functional food products but also as a source of natural antitumour drugs.
28578968	9	28	contain	possess	1760:1766	arg2	potential					1768:1776	potential	1768:1776	potential not only for use in functional food products but also as a source of natural antitumour drugs	1768:1870	Our results suggested that LW1 and LW2 possess potential not only for use in functional food products but also as a source of natural antitumour drugs.
28578968	9	28	contain	possess	1760:1766	arg1	LW2					1756:1758	LW2	1756:1758	LW2	1756:1758	Our results suggested that LW1 and LW2 possess potential not only for use in functional food products but also as a source of natural antitumour drugs.
28578968	5	29	theme	LW1	866:868	arg1	chain					857:861	the main chain	848:861	the main chain of LW1	848:868	Methylation results showed that the main chain of LW1 likely involves (1→4)-linked Galp and (1→4)-linked Glcp with its side chains being (1→4,6)-linked Galp through the O-6 position connected to the backbone, whereas the main chain of LW2 likely involves (1→4)-linked Galp and (1→4)-linked Glcp with its side chains being (1→3)-Galp through the O-6 position of (1→3,6)-Galp linked to the main chain.
28578968	5	30	theme	LW2	1051:1053	arg1	chain					1042:1046	the main chain	1033:1046	the main chain of LW2	1033:1053	Methylation results showed that the main chain of LW1 likely involves (1→4)-linked Galp and (1→4)-linked Glcp with its side chains being (1→4,6)-linked Galp through the O-6 position connected to the backbone, whereas the main chain of LW2 likely involves (1→4)-linked Galp and (1→4)-linked Glcp with its side chains being (1→3)-Galp through the O-6 position of (1→3,6)-Galp linked to the main chain.
28578968	7	31	theme	antitumour	1419:1428	arg1	tests					1430:1434	in vitro antitumour tests	1410:1434	in vitro antitumour tests	1410:1434	The results from in vitro antitumour tests indicated that both LW1 and LW2 could significantly inhibit the proliferation of HT-29 colorectal cancer cells and up-regulated the expressions of Bad, Bax, Caspase-3 and -8 genes.
28578968	0	32	theme	Physicochemical	0:14	arg1	characterization					16:31	Physicochemical characterization	0:31	Physicochemical characterization	0:31	Physicochemical characterization and antitumour activity of exopolysaccharides produced by Lactobacillus casei SB27 from yak milk.
28578968	7	33	theme	in	1410:1411	arg1	tests					1430:1434	in vitro antitumour tests	1410:1434	in vitro antitumour tests	1410:1434	The results from in vitro antitumour tests indicated that both LW1 and LW2 could significantly inhibit the proliferation of HT-29 colorectal cancer cells and up-regulated the expressions of Bad, Bax, Caspase-3 and -8 genes.
28578968	2	34	theme	physicochemical	421:435	arg1	characterization					437:452	the physicochemical characterization	417:452	the physicochemical characterization of these two fractions	417:475	GC-MS, FTIR spectroscopy, methylation analysis and FE-SEM analysis were performed to elucidate the physicochemical characterization of these two fractions, and their in vitro antitumour activities were also evaluated.
28578968	1	35	dep	fractions	157:165	arg1	LW2					176:178	LW2	176:178	LW2	176:178	Two high molecular weight fractions (LW1 and LW2) of exopolysaccharides (EPSs) produced by Lactobacillus casei SB27 were isolated from yak milk obtained from the Gansu Tibetan area of China.
28578968	1	35	dep	fractions	157:165	arg1	LW1					168:170	LW1	168:170	LW1	168:170	Two high molecular weight fractions (LW1 and LW2) of exopolysaccharides (EPSs) produced by Lactobacillus casei SB27 were isolated from yak milk obtained from the Gansu Tibetan area of China.
28578968	1	35	dep	fractions	157:165	arg1	fractions					157:165	Two high molecular weight fractions	131:165	Two high molecular weight fractions (LW1 and LW2) of exopolysaccharides (EPSs) produced by Lactobacillus casei SB27	131:245	Two high molecular weight fractions (LW1 and LW2) of exopolysaccharides (EPSs) produced by Lactobacillus casei SB27 were isolated from yak milk obtained from the Gansu Tibetan area of China.
28578968	5	36	theme	side	1120:1123	arg1	chains					1125:1130	its side chains	1116:1130	its side chains	1116:1130	Methylation results showed that the main chain of LW1 likely involves (1→4)-linked Galp and (1→4)-linked Glcp with its side chains being (1→4,6)-linked Galp through the O-6 position connected to the backbone, whereas the main chain of LW2 likely involves (1→4)-linked Galp and (1→4)-linked Glcp with its side chains being (1→3)-Galp through the O-6 position of (1→3,6)-Galp linked to the main chain.
28578968	1	37	theme	Gansu	293:297	arg1	area					307:310	the Gansu Tibetan area	289:310	the Gansu Tibetan area of China	289:319	Two high molecular weight fractions (LW1 and LW2) of exopolysaccharides (EPSs) produced by Lactobacillus casei SB27 were isolated from yak milk obtained from the Gansu Tibetan area of China.
28578968	0	38	theme	exopolysaccharides	60:77	arg1	characterization					16:31	Physicochemical characterization	0:31	Physicochemical characterization	0:31	Physicochemical characterization and antitumour activity of exopolysaccharides produced by Lactobacillus casei SB27 from yak milk.
28578968	0	38	theme	exopolysaccharides	60:77	arg1	activity					48:55	antitumour activity	37:55	antitumour activity	37:55	Physicochemical characterization and antitumour activity of exopolysaccharides produced by Lactobacillus casei SB27 from yak milk.
28578968	5	39	theme	1→4	909:911	arg1	Glcp					921:924	(1→4)-linked Glcp	908:924	(1→4)-linked Glcp	908:924	Methylation results showed that the main chain of LW1 likely involves (1→4)-linked Galp and (1→4)-linked Glcp with its side chains being (1→4,6)-linked Galp through the O-6 position connected to the backbone, whereas the main chain of LW2 likely involves (1→4)-linked Galp and (1→4)-linked Glcp with its side chains being (1→3)-Galp through the O-6 position of (1→3,6)-Galp linked to the main chain.
28578968	9	40	theme	food	1809:1812	arg1	products					1814:1821	functional food products	1798:1821	functional food products	1798:1821	Our results suggested that LW1 and LW2 possess potential not only for use in functional food products but also as a source of natural antitumour drugs.
28578968	7	41	theme	-8	1607:1608	arg1	expressions					1568:1578	the expressions	1564:1578	the expressions of Bad, Bax, Caspase-3 and -8 genes	1564:1614	The results from in vitro antitumour tests indicated that both LW1 and LW2 could significantly inhibit the proliferation of HT-29 colorectal cancer cells and up-regulated the expressions of Bad, Bax, Caspase-3 and -8 genes.
28578968	5	42	theme	1→4	887:889	arg1	Galp					899:902	(1→4)-linked Galp	886:902	(1→4)-linked Galp	886:902	Methylation results showed that the main chain of LW1 likely involves (1→4)-linked Galp and (1→4)-linked Glcp with its side chains being (1→4,6)-linked Galp through the O-6 position connected to the backbone, whereas the main chain of LW2 likely involves (1→4)-linked Galp and (1→4)-linked Glcp with its side chains being (1→3)-Galp through the O-6 position of (1→3,6)-Galp linked to the main chain.
28578968	6	43	dep	morphology	1246:1255	arg1	revealed					1261:1268	revealed	1261:1268	revealed by FE-SEM analysis of the two EPS fractions	1261:1312	Evaluation of the microcosmic morphology, as revealed by FE-SEM analysis of the two EPS fractions, showed a sheet-like appearance with a folded surface and a compact structure.
28578968	4	44	theme	mol	759:761	arg1	%					746:746	52.4%	742:746	52.4%	742:746	Monosaccharide composition analysis revealed that LW1 and LW2 were mainly composed of galactose (52.4% and 57.4%, mol%) and glucose (29.1% and 22.2%, mol%), respectively.
28578968	4	44	theme	mol	759:761	arg1	%					762:762	mol%	759:762	mol%	759:762	Monosaccharide composition analysis revealed that LW1 and LW2 were mainly composed of galactose (52.4% and 57.4%, mol%) and glucose (29.1% and 22.2%, mol%), respectively.
28578968	2	45	theme	methylation	348:358	arg1	analysis					360:367	methylation analysis	348:367	methylation analysis	348:367	GC-MS, FTIR spectroscopy, methylation analysis and FE-SEM analysis were performed to elucidate the physicochemical characterization of these two fractions, and their in vitro antitumour activities were also evaluated.
28578968	6	46	theme	microcosmic	1234:1244	arg1	morphology					1246:1255	the microcosmic morphology	1230:1255	the microcosmic morphology	1230:1255	Evaluation of the microcosmic morphology, as revealed by FE-SEM analysis of the two EPS fractions, showed a sheet-like appearance with a folded surface and a compact structure.
28578968	5	47	theme	-linked	891:897	arg1	Galp					899:902	(1→4)-linked Galp	886:902	(1→4)-linked Galp	886:902	Methylation results showed that the main chain of LW1 likely involves (1→4)-linked Galp and (1→4)-linked Glcp with its side chains being (1→4,6)-linked Galp through the O-6 position connected to the backbone, whereas the main chain of LW2 likely involves (1→4)-linked Galp and (1→4)-linked Glcp with its side chains being (1→3)-Galp through the O-6 position of (1→3,6)-Galp linked to the main chain.
28578968	5	48	theme	main	1037:1040	arg1	chain					1042:1046	the main chain	1033:1046	the main chain of LW2	1033:1053	Methylation results showed that the main chain of LW1 likely involves (1→4)-linked Galp and (1→4)-linked Glcp with its side chains being (1→4,6)-linked Galp through the O-6 position connected to the backbone, whereas the main chain of LW2 likely involves (1→4)-linked Galp and (1→4)-linked Glcp with its side chains being (1→3)-Galp through the O-6 position of (1→3,6)-Galp linked to the main chain.
28578968	7	49	theme	cancer	1534:1539	arg1	cells					1541:1545	HT-29 colorectal cancer cells	1517:1545	HT-29 colorectal cancer cells	1517:1545	The results from in vitro antitumour tests indicated that both LW1 and LW2 could significantly inhibit the proliferation of HT-29 colorectal cancer cells and up-regulated the expressions of Bad, Bax, Caspase-3 and -8 genes.
28578968	9	50	theme	natural	1847:1853	arg1	drugs					1866:1870	natural antitumour drugs	1847:1870	natural antitumour drugs	1847:1870	Our results suggested that LW1 and LW2 possess potential not only for use in functional food products but also as a source of natural antitumour drugs.
28578968	7	51	theme	HT-29	1517:1521	arg1	cells					1541:1545	HT-29 colorectal cancer cells	1517:1545	HT-29 colorectal cancer cells	1517:1545	The results from in vitro antitumour tests indicated that both LW1 and LW2 could significantly inhibit the proliferation of HT-29 colorectal cancer cells and up-regulated the expressions of Bad, Bax, Caspase-3 and -8 genes.
28578968	9	52	theme	drugs	1866:1870	arg1	source					1837:1842	a source	1835:1842	a source of natural antitumour drugs	1835:1870	Our results suggested that LW1 and LW2 possess potential not only for use in functional food products but also as a source of natural antitumour drugs.
28578968	5	53	link	-linked	891:897	arg1	Galp					899:902	(1→4)-linked Galp	886:902	(1→4)-linked Galp	886:902	Methylation results showed that the main chain of LW1 likely involves (1→4)-linked Galp and (1→4)-linked Glcp with its side chains being (1→4,6)-linked Galp through the O-6 position connected to the backbone, whereas the main chain of LW2 likely involves (1→4)-linked Galp and (1→4)-linked Glcp with its side chains being (1→3)-Galp through the O-6 position of (1→3,6)-Galp linked to the main chain.
28578968	6	54	theme	FE-SEM	1273:1278	arg1	analysis					1280:1287	FE-SEM analysis	1273:1287	FE-SEM analysis of the two EPS fractions	1273:1312	Evaluation of the microcosmic morphology, as revealed by FE-SEM analysis of the two EPS fractions, showed a sheet-like appearance with a folded surface and a compact structure.
28578968	4	55	dep	glucose	769:775	arg1	%					782:782	29.1%	778:782	29.1%	778:782	Monosaccharide composition analysis revealed that LW1 and LW2 were mainly composed of galactose (52.4% and 57.4%, mol%) and glucose (29.1% and 22.2%, mol%), respectively.
28578968	4	55	dep	glucose	769:775	arg1	%					792:792	22.2%	788:792	22.2%	788:792	Monosaccharide composition analysis revealed that LW1 and LW2 were mainly composed of galactose (52.4% and 57.4%, mol%) and glucose (29.1% and 22.2%, mol%), respectively.
28578968	4	55	dep	glucose	769:775	arg1	%					798:798	mol%	795:798	mol%	795:798	Monosaccharide composition analysis revealed that LW1 and LW2 were mainly composed of galactose (52.4% and 57.4%, mol%) and glucose (29.1% and 22.2%, mol%), respectively.
28578968	8	56	theme	morphological	1660:1672	arg1	changes					1674:1680	the apoptotic morphological changes	1646:1680	the apoptotic morphological changes of HT-29 cells induced by LW1 and LW2	1646:1718	Finally, TEM images revealed the apoptotic morphological changes of HT-29 cells induced by LW1 and LW2.
28578968	5	57	theme	side	935:938	arg1	chains					940:945	its side chains	931:945	its side chains	931:945	Methylation results showed that the main chain of LW1 likely involves (1→4)-linked Galp and (1→4)-linked Glcp with its side chains being (1→4,6)-linked Galp through the O-6 position connected to the backbone, whereas the main chain of LW2 likely involves (1→4)-linked Galp and (1→4)-linked Glcp with its side chains being (1→3)-Galp through the O-6 position of (1→3,6)-Galp linked to the main chain.
28578968	9	58	from	use	1791:1793	arg1	products					1814:1821	functional food products	1798:1821	functional food products	1798:1821	Our results suggested that LW1 and LW2 possess potential not only for use in functional food products but also as a source of natural antitumour drugs.
28578968	2	59	dep	in	488:489	arg1	vitro					491:495	vitro	491:495	vitro	491:495	GC-MS, FTIR spectroscopy, methylation analysis and FE-SEM analysis were performed to elucidate the physicochemical characterization of these two fractions, and their in vitro antitumour activities were also evaluated.
28578968	2	59	dep	in	488:489	arg1	antitumour					497:506	antitumour	497:506	antitumour	497:506	GC-MS, FTIR spectroscopy, methylation analysis and FE-SEM analysis were performed to elucidate the physicochemical characterization of these two fractions, and their in vitro antitumour activities were also evaluated.
28578968	4	60	dep	galactose	731:739	arg1	%					746:746	52.4%	742:746	52.4%	742:746	Monosaccharide composition analysis revealed that LW1 and LW2 were mainly composed of galactose (52.4% and 57.4%, mol%) and glucose (29.1% and 22.2%, mol%), respectively.
28578968	4	60	dep	galactose	731:739	arg1	%					756:756	57.4%	752:756	57.4%	752:756	Monosaccharide composition analysis revealed that LW1 and LW2 were mainly composed of galactose (52.4% and 57.4%, mol%) and glucose (29.1% and 22.2%, mol%), respectively.
28578968	4	60	dep	galactose	731:739	arg1	%					762:762	mol%	759:762	mol%	759:762	Monosaccharide composition analysis revealed that LW1 and LW2 were mainly composed of galactose (52.4% and 57.4%, mol%) and glucose (29.1% and 22.2%, mol%), respectively.
28578968	8	61	theme	cells	1691:1695	arg1	changes					1674:1680	the apoptotic morphological changes	1646:1680	the apoptotic morphological changes of HT-29 cells induced by LW1 and LW2	1646:1718	Finally, TEM images revealed the apoptotic morphological changes of HT-29 cells induced by LW1 and LW2.
28578968	5	62	theme	-linked	913:919	arg1	Glcp					921:924	(1→4)-linked Glcp	908:924	(1→4)-linked Glcp	908:924	Methylation results showed that the main chain of LW1 likely involves (1→4)-linked Galp and (1→4)-linked Glcp with its side chains being (1→4,6)-linked Galp through the O-6 position connected to the backbone, whereas the main chain of LW2 likely involves (1→4)-linked Galp and (1→4)-linked Glcp with its side chains being (1→3)-Galp through the O-6 position of (1→3,6)-Galp linked to the main chain.
28578968	1	63	attach	isolated	252:259	arg2	LW2					176:178	LW2	176:178	LW2	176:178	Two high molecular weight fractions (LW1 and LW2) of exopolysaccharides (EPSs) produced by Lactobacillus casei SB27 were isolated from yak milk obtained from the Gansu Tibetan area of China.
28578968	1	63	attach	isolated	252:259	arg1	milk					270:273	yak milk	266:273	yak milk obtained from the Gansu Tibetan area of China	266:319	Two high molecular weight fractions (LW1 and LW2) of exopolysaccharides (EPSs) produced by Lactobacillus casei SB27 were isolated from yak milk obtained from the Gansu Tibetan area of China.
28578968	1	63	attach	isolated	252:259	arg2	LW1					168:170	LW1	168:170	LW1	168:170	Two high molecular weight fractions (LW1 and LW2) of exopolysaccharides (EPSs) produced by Lactobacillus casei SB27 were isolated from yak milk obtained from the Gansu Tibetan area of China.
28578968	1	63	attach	isolated	252:259	arg2	fractions					157:165	Two high molecular weight fractions	131:165	Two high molecular weight fractions (LW1 and LW2) of exopolysaccharides (EPSs) produced by Lactobacillus casei SB27	131:245	Two high molecular weight fractions (LW1 and LW2) of exopolysaccharides (EPSs) produced by Lactobacillus casei SB27 were isolated from yak milk obtained from the Gansu Tibetan area of China.
28578968	6	64	theme	sheet-like	1324:1333	arg1	appearance					1335:1344	a sheet-like appearance	1322:1344	a sheet-like appearance	1322:1344	Evaluation of the microcosmic morphology, as revealed by FE-SEM analysis of the two EPS fractions, showed a sheet-like appearance with a folded surface and a compact structure.
28578968	1	65	theme	molecular	140:148	arg1	weight					150:155	high molecular weight	135:155	Two high molecular weight fractions (LW1 and LW2) of exopolysaccharides (EPSs) produced by Lactobacillus casei SB27	131:245	Two high molecular weight fractions (LW1 and LW2) of exopolysaccharides (EPSs) produced by Lactobacillus casei SB27 were isolated from yak milk obtained from the Gansu Tibetan area of China.
28578968	7	66	from	tests	1430:1434	arg1	results					1397:1403	The results	1393:1403	The results from in vitro antitumour tests	1393:1434	The results from in vitro antitumour tests indicated that both LW1 and LW2 could significantly inhibit the proliferation of HT-29 colorectal cancer cells and up-regulated the expressions of Bad, Bax, Caspase-3 and -8 genes.
28578968	0	67	theme	Lactobacillus	91:103	arg1	casei					105:109	Lactobacillus casei SB27	91:114	Lactobacillus casei SB27	91:114	Physicochemical characterization and antitumour activity of exopolysaccharides produced by Lactobacillus casei SB27 from yak milk.
28578968	3	68	dep	weights	554:560	arg1	determined					586:595	determined	586:595	determined by HPGPC	586:604	The molecular weights (Mws) of LW1 and LW2 as determined by HPGPC were 25.10 and 12.34kDa, respectively.
28578968	5	69	link	-linked	913:919	arg1	Glcp					921:924	(1→4)-linked Glcp	908:924	(1→4)-linked Glcp	908:924	Methylation results showed that the main chain of LW1 likely involves (1→4)-linked Galp and (1→4)-linked Glcp with its side chains being (1→4,6)-linked Galp through the O-6 position connected to the backbone, whereas the main chain of LW2 likely involves (1→4)-linked Galp and (1→4)-linked Glcp with its side chains being (1→3)-Galp through the O-6 position of (1→3,6)-Galp linked to the main chain.
28578968	2	70	theme	in	488:489	arg1	activities					508:517	their in vitro antitumour activities	482:517	their in vitro antitumour activities	482:517	GC-MS, FTIR spectroscopy, methylation analysis and FE-SEM analysis were performed to elucidate the physicochemical characterization of these two fractions, and their in vitro antitumour activities were also evaluated.
28578968	7	71	dep	in	1410:1411	arg1	vitro					1413:1417	vitro	1413:1417	vitro	1413:1417	The results from in vitro antitumour tests indicated that both LW1 and LW2 could significantly inhibit the proliferation of HT-29 colorectal cancer cells and up-regulated the expressions of Bad, Bax, Caspase-3 and -8 genes.
28578968	4	72	theme	composition	660:670	arg1	analysis					672:679	Monosaccharide composition analysis	645:679	Monosaccharide composition analysis	645:679	Monosaccharide composition analysis revealed that LW1 and LW2 were mainly composed of galactose (52.4% and 57.4%, mol%) and glucose (29.1% and 22.2%, mol%), respectively.
28578968	6	73	theme	fractions	1304:1312	arg1	analysis					1280:1287	FE-SEM analysis	1273:1287	FE-SEM analysis of the two EPS fractions	1273:1312	Evaluation of the microcosmic morphology, as revealed by FE-SEM analysis of the two EPS fractions, showed a sheet-like appearance with a folded surface and a compact structure.
28578968	0	74	theme	yak	121:123	arg1	milk					125:128	yak milk	121:128	yak milk	121:128	Physicochemical characterization and antitumour activity of exopolysaccharides produced by Lactobacillus casei SB27 from yak milk.
28578968	5	75	theme	O-6	985:987	arg1	position					989:996	the O-6 position	981:996	the O-6 position connected to the backbone	981:1022	Methylation results showed that the main chain of LW1 likely involves (1→4)-linked Galp and (1→4)-linked Glcp with its side chains being (1→4,6)-linked Galp through the O-6 position connected to the backbone, whereas the main chain of LW2 likely involves (1→4)-linked Galp and (1→4)-linked Glcp with its side chains being (1→3)-Galp through the O-6 position of (1→3,6)-Galp linked to the main chain.
28578968	6	76	theme	compact	1374:1380	arg1	structure					1382:1390	a compact structure	1372:1390	a compact structure	1372:1390	Evaluation of the microcosmic morphology, as revealed by FE-SEM analysis of the two EPS fractions, showed a sheet-like appearance with a folded surface and a compact structure.
28578968	5	77	link	-linked	960:966	arg1	Galp					968:971	(1→4,6)-linked Galp	953:971	(1→4,6)-linked Galp	953:971	Methylation results showed that the main chain of LW1 likely involves (1→4)-linked Galp and (1→4)-linked Glcp with its side chains being (1→4,6)-linked Galp through the O-6 position connected to the backbone, whereas the main chain of LW2 likely involves (1→4)-linked Galp and (1→4)-linked Glcp with its side chains being (1→3)-Galp through the O-6 position of (1→3,6)-Galp linked to the main chain.
28578968	2	78	theme	FE-SEM	373:378	arg1	analysis					380:387	FE-SEM analysis	373:387	FE-SEM analysis	373:387	GC-MS, FTIR spectroscopy, methylation analysis and FE-SEM analysis were performed to elucidate the physicochemical characterization of these two fractions, and their in vitro antitumour activities were also evaluated.
28578968	5	79	theme	-linked	960:966	arg1	Galp					968:971	(1→4,6)-linked Galp	953:971	(1→4,6)-linked Galp	953:971	Methylation results showed that the main chain of LW1 likely involves (1→4)-linked Galp and (1→4)-linked Glcp with its side chains being (1→4,6)-linked Galp through the O-6 position connected to the backbone, whereas the main chain of LW2 likely involves (1→4)-linked Galp and (1→4)-linked Glcp with its side chains being (1→3)-Galp through the O-6 position of (1→3,6)-Galp linked to the main chain.
28578968	0	80	theme	antitumour	37:46	arg1	activity					48:55	antitumour activity	37:55	antitumour activity	37:55	Physicochemical characterization and antitumour activity of exopolysaccharides produced by Lactobacillus casei SB27 from yak milk.
28578968	6	81	theme	folded	1353:1358	arg1	surface					1360:1366	a folded surface	1351:1366	a folded surface	1351:1366	Evaluation of the microcosmic morphology, as revealed by FE-SEM analysis of the two EPS fractions, showed a sheet-like appearance with a folded surface and a compact structure.
28578968	5	82	theme	main	852:855	arg1	chain					857:861	the main chain	848:861	the main chain of LW1	848:868	Methylation results showed that the main chain of LW1 likely involves (1→4)-linked Galp and (1→4)-linked Glcp with its side chains being (1→4,6)-linked Galp through the O-6 position connected to the backbone, whereas the main chain of LW2 likely involves (1→4)-linked Galp and (1→4)-linked Glcp with its side chains being (1→3)-Galp through the O-6 position of (1→3,6)-Galp linked to the main chain.
28578968	2	83	theme	fractions	467:475	arg1	characterization					437:452	the physicochemical characterization	417:452	the physicochemical characterization of these two fractions	417:475	GC-MS, FTIR spectroscopy, methylation analysis and FE-SEM analysis were performed to elucidate the physicochemical characterization of these two fractions, and their in vitro antitumour activities were also evaluated.
28578968	5	84	theme	O-6	1161:1163	arg1	position					1165:1172	the O-6 position	1157:1172	the O-6 position of (1→3,6)-Galp linked to the main chain	1157:1213	Methylation results showed that the main chain of LW1 likely involves (1→4)-linked Galp and (1→4)-linked Glcp with its side chains being (1→4,6)-linked Galp through the O-6 position connected to the backbone, whereas the main chain of LW2 likely involves (1→4)-linked Galp and (1→4)-linked Glcp with its side chains being (1→3)-Galp through the O-6 position of (1→3,6)-Galp linked to the main chain.
28578968	5	85	attach	linked	1190:1195	arg1	chain					1209:1213	the main chain	1200:1213	the main chain	1200:1213	Methylation results showed that the main chain of LW1 likely involves (1→4)-linked Galp and (1→4)-linked Glcp with its side chains being (1→4,6)-linked Galp through the O-6 position connected to the backbone, whereas the main chain of LW2 likely involves (1→4)-linked Galp and (1→4)-linked Glcp with its side chains being (1→3)-Galp through the O-6 position of (1→3,6)-Galp linked to the main chain.
28578968	5	85	attach	linked	1190:1195	arg2	-Galp					1184:1188	(1→3,6)-Galp	1177:1188	(1→3,6)-Galp linked to the main chain	1177:1213	Methylation results showed that the main chain of LW1 likely involves (1→4)-linked Galp and (1→4)-linked Glcp with its side chains being (1→4,6)-linked Galp through the O-6 position connected to the backbone, whereas the main chain of LW2 likely involves (1→4)-linked Galp and (1→4)-linked Glcp with its side chains being (1→3)-Galp through the O-6 position of (1→3,6)-Galp linked to the main chain.
28578968	1	86	theme	Tibetan	299:305	arg1	area					307:310	the Gansu Tibetan area	289:310	the Gansu Tibetan area of China	289:319	Two high molecular weight fractions (LW1 and LW2) of exopolysaccharides (EPSs) produced by Lactobacillus casei SB27 were isolated from yak milk obtained from the Gansu Tibetan area of China.
28578968	7	87	theme	Caspase-3	1593:1601	arg1	expressions					1568:1578	the expressions	1564:1578	the expressions of Bad, Bax, Caspase-3 and -8 genes	1564:1614	The results from in vitro antitumour tests indicated that both LW1 and LW2 could significantly inhibit the proliferation of HT-29 colorectal cancer cells and up-regulated the expressions of Bad, Bax, Caspase-3 and -8 genes.
28578968	9	88	theme	functional	1798:1807	arg1	products					1814:1821	functional food products	1798:1821	functional food products	1798:1821	Our results suggested that LW1 and LW2 possess potential not only for use in functional food products but also as a source of natural antitumour drugs.
28578968	7	89	theme	Bax	1588:1590	arg1	expressions					1568:1578	the expressions	1564:1578	the expressions of Bad, Bax, Caspase-3 and -8 genes	1564:1614	The results from in vitro antitumour tests indicated that both LW1 and LW2 could significantly inhibit the proliferation of HT-29 colorectal cancer cells and up-regulated the expressions of Bad, Bax, Caspase-3 and -8 genes.
28578968	6	90	theme	morphology	1246:1255	arg1	Evaluation					1216:1225	Evaluation	1216:1225	Evaluation of the microcosmic morphology, as revealed by FE-SEM analysis of the two EPS fractions,	1216:1313	Evaluation of the microcosmic morphology, as revealed by FE-SEM analysis of the two EPS fractions, showed a sheet-like appearance with a folded surface and a compact structure.
28578968	1	91	theme	Lactobacillus	222:234	arg1	casei					236:240	Lactobacillus casei SB27	222:245	Lactobacillus casei SB27	222:245	Two high molecular weight fractions (LW1 and LW2) of exopolysaccharides (EPSs) produced by Lactobacillus casei SB27 were isolated from yak milk obtained from the Gansu Tibetan area of China.
28578968	7	92	theme	Bad	1583:1585	arg1	expressions					1568:1578	the expressions	1564:1578	the expressions of Bad, Bax, Caspase-3 and -8 genes	1564:1614	The results from in vitro antitumour tests indicated that both LW1 and LW2 could significantly inhibit the proliferation of HT-29 colorectal cancer cells and up-regulated the expressions of Bad, Bax, Caspase-3 and -8 genes.
28578968	5	93	theme	1→4	1072:1074	arg1	Galp					1084:1087	(1→4)-linked Galp	1071:1087	(1→4)-linked Galp	1071:1087	Methylation results showed that the main chain of LW1 likely involves (1→4)-linked Galp and (1→4)-linked Glcp with its side chains being (1→4,6)-linked Galp through the O-6 position connected to the backbone, whereas the main chain of LW2 likely involves (1→4)-linked Galp and (1→4)-linked Glcp with its side chains being (1→3)-Galp through the O-6 position of (1→3,6)-Galp linked to the main chain.
27062721	4	0	theme	simple	1001:1006	arg1	assignment					1048:1057	a simple and accurate structural oligosaccharide assignment	999:1057	a simple and accurate structural oligosaccharide assignment	999:1057	The application of a polar solvent having a high pH with acetonitrile avoided desulfation enabling a simple and accurate structural oligosaccharide assignment.
27062721	1	1	theme	Oligosaccharide	215:229	arg1	mapping					231:237	Oligosaccharide mapping	215:237	Oligosaccharide mapping based on enzyme cleavage	215:262	Oligosaccharide mapping based on enzyme cleavage provides a useful molecular fingerprint of the heparin structure revealing detailed structural information regarding its sequence and the content of part of the ATIII-binding region.
27062721	3	2	theme	fluorescence	849:860	arg1	detector					862:869	a fluorescence detector	847:869	a fluorescence detector	847:869	We report a novel oligosaccharide mapping strategy based on the HILIC separation of the main heparin disaccharides/oligosaccharides released by heparinase I, fluorotagged with 2-aminoacridone and on-line detected by a fluorescence detector and characterized by ESI-MS.
27062721	1	3	theme	structure	319:327	arg1	fingerprint					292:302	a useful molecular fingerprint	273:302	a useful molecular fingerprint of the heparin structure revealing detailed structural information regarding its sequence and the content of part of the ATIII-binding region	273:444	Oligosaccharide mapping based on enzyme cleavage provides a useful molecular fingerprint of the heparin structure revealing detailed structural information regarding its sequence and the content of part of the ATIII-binding region.
27062721	0	4	theme	chromatography	91:104	arg1	separation					106:115	hydrophilic interaction liquid chromatography separation	60:115	hydrophilic interaction liquid chromatography separation	60:115	Oligosaccharide mapping of heparinase I-treated heparins by hydrophilic interaction liquid chromatography separation and online fluorescence detection and electrospray ionization-mass spectrometry characterization.
27062721	7	5	theme	HILIC	1404:1408	arg1	approach					1410:1417	this novel HILIC approach	1393:1417	this novel HILIC approach	1393:1417	Contrary to SAX-HPLC, this novel HILIC approach is able to separate and identify the saturated trisulfated disaccharide belonging to the non-reducing end of heparin chains.
27062721	0	6	theme	online	121:126	arg1	detection					141:149	online fluorescence detection	121:149	online fluorescence detection	121:149	Oligosaccharide mapping of heparinase I-treated heparins by hydrophilic interaction liquid chromatography separation and online fluorescence detection and electrospray ionization-mass spectrometry characterization.
27062721	1	7	theme	ATIII-binding	425:437	arg1	region					439:444	the ATIII-binding region	421:444	the ATIII-binding region	421:444	Oligosaccharide mapping based on enzyme cleavage provides a useful molecular fingerprint of the heparin structure revealing detailed structural information regarding its sequence and the content of part of the ATIII-binding region.
27062721	4	8	theme	accurate	1012:1019	arg1	assignment					1048:1057	a simple and accurate structural oligosaccharide assignment	999:1057	a simple and accurate structural oligosaccharide assignment	999:1057	The application of a polar solvent having a high pH with acetonitrile avoided desulfation enabling a simple and accurate structural oligosaccharide assignment.
27062721	8	9	theme	3-O-sulfo	1568:1576	arg1	region					1606:1611	the ATIII-binding region	1588:1611	the ATIII-binding region	1588:1611	Finally, the content of 3-O-sulfo groups of the ATIII-binding region is determined.
27062721	8	9	theme	3-O-sulfo	1568:1576	arg1	groups					1578:1583	3-O-sulfo groups	1568:1583	3-O-sulfo groups of the ATIII-binding region	1568:1611	Finally, the content of 3-O-sulfo groups of the ATIII-binding region is determined.
27062721	0	10	dep	detection	141:149	arg1	characterization					197:212	characterization	197:212	characterization	197:212	Oligosaccharide mapping of heparinase I-treated heparins by hydrophilic interaction liquid chromatography separation and online fluorescence detection and electrospray ionization-mass spectrometry characterization.
27062721	1	11	theme	detailed	339:346	arg1	information					359:369	detailed structural information	339:369	detailed structural information regarding its sequence and the content of part of the ATIII-binding region	339:444	Oligosaccharide mapping based on enzyme cleavage provides a useful molecular fingerprint of the heparin structure revealing detailed structural information regarding its sequence and the content of part of the ATIII-binding region.
27062721	1	12	theme	region	439:444	arg1	part					413:416	part	413:416	part of the ATIII-binding region	413:444	Oligosaccharide mapping based on enzyme cleavage provides a useful molecular fingerprint of the heparin structure revealing detailed structural information regarding its sequence and the content of part of the ATIII-binding region.
27062721	6	13	theme	heparinases	1347:1357	arg1	use					1330:1332	the use	1326:1332	the use of expensive heparinases II and III	1326:1368	Additionally, only widely commercially available heparin lyase I is necessary, without the use of expensive heparinases II and III.
27062721	3	14	theme	novel	643:647	arg1	strategy					673:680	a novel oligosaccharide mapping strategy	641:680	a novel oligosaccharide mapping strategy based on the HILIC separation of the main heparin disaccharides/oligosaccharides released by heparinase I, fluorotagged with 2-aminoacridone and on-line detected by a fluorescence detector and characterized by ESI-MS	641:897	We report a novel oligosaccharide mapping strategy based on the HILIC separation of the main heparin disaccharides/oligosaccharides released by heparinase I, fluorotagged with 2-aminoacridone and on-line detected by a fluorescence detector and characterized by ESI-MS.
27062721	1	15	theme	structural	348:357	arg1	information					359:369	detailed structural information	339:369	detailed structural information regarding its sequence and the content of part of the ATIII-binding region	339:444	Oligosaccharide mapping based on enzyme cleavage provides a useful molecular fingerprint of the heparin structure revealing detailed structural information regarding its sequence and the content of part of the ATIII-binding region.
27062721	2	16	theme	conclusive	604:613	arg1	identification					615:628	their conclusive identification	598:628	their conclusive identification	598:628	This approach is performed by strong-anion exchange (SAX)-HPLC separation which is incompatible with MS requiring purification of oligosaccharides for their conclusive identification.
27062721	7	17	theme	trisulfated	1466:1476	arg1	disaccharide					1478:1489	the saturated trisulfated disaccharide	1452:1489	the saturated trisulfated disaccharide belonging to the non-reducing end of heparin chains	1452:1541	Contrary to SAX-HPLC, this novel HILIC approach is able to separate and identify the saturated trisulfated disaccharide belonging to the non-reducing end of heparin chains.
27062721	6	18	theme	expensive	1337:1345	arg1	heparinases					1347:1357	expensive heparinases II and III	1337:1368	expensive heparinases II and III	1337:1368	Additionally, only widely commercially available heparin lyase I is necessary, without the use of expensive heparinases II and III.
27062721	6	18	theme	expensive	1337:1345	arg1	III					1366:1368	III	1366:1368	III	1366:1368	Additionally, only widely commercially available heparin lyase I is necessary, without the use of expensive heparinases II and III.
27062721	6	18	theme	expensive	1337:1345	arg1	II					1359:1360	II	1359:1360	II	1359:1360	Additionally, only widely commercially available heparin lyase I is necessary, without the use of expensive heparinases II and III.
27062721	2	19	theme	strong-anion	477:488	arg1	separation					510:519	strong-anion exchange (SAX)-HPLC separation	477:519	strong-anion exchange (SAX)-HPLC separation which is incompatible with MS requiring purification of oligosaccharides for their conclusive identification	477:628	This approach is performed by strong-anion exchange (SAX)-HPLC separation which is incompatible with MS requiring purification of oligosaccharides for their conclusive identification.
27062721	0	20	theme	fluorescence	128:139	arg1	detection					141:149	online fluorescence detection	121:149	online fluorescence detection	121:149	Oligosaccharide mapping of heparinase I-treated heparins by hydrophilic interaction liquid chromatography separation and online fluorescence detection and electrospray ionization-mass spectrometry characterization.
27062721	4	21	with	pH	949:950	arg1	acetonitrile					957:968	acetonitrile	957:968	acetonitrile	957:968	The application of a polar solvent having a high pH with acetonitrile avoided desulfation enabling a simple and accurate structural oligosaccharide assignment.
27062721	8	22	theme	groups	1578:1583	arg1	content					1557:1563	the content	1553:1563	the content of 3-O-sulfo groups of the ATIII-binding region	1553:1611	Finally, the content of 3-O-sulfo groups of the ATIII-binding region is determined.
27062721	1	23	theme	enzyme	248:253	arg1	cleavage					255:262	enzyme cleavage	248:262	enzyme cleavage	248:262	Oligosaccharide mapping based on enzyme cleavage provides a useful molecular fingerprint of the heparin structure revealing detailed structural information regarding its sequence and the content of part of the ATIII-binding region.
27062721	0	24	theme	Oligosaccharide	0:14	arg1	mapping					16:22	Oligosaccharide mapping	0:22	Oligosaccharide mapping of heparinase I-treated heparins by hydrophilic interaction liquid chromatography separation	0:115	Oligosaccharide mapping of heparinase I-treated heparins by hydrophilic interaction liquid chromatography separation and online fluorescence detection and electrospray ionization-mass spectrometry characterization.
27062721	5	25	theme	fluorescence	1172:1183	arg1	detector					1185:1192	the fluorescence detector	1168:1192	the fluorescence detector	1168:1192	Oligosaccharide mapping, or merely complete disaccharide composition, may be performed on nanogram-scale by the fluorescence detector vs micrograms useful for classical SAX-HPLC.
27062721	5	26	theme	complete	1095:1102	arg1	composition					1117:1127	complete disaccharide composition	1095:1127	complete disaccharide composition	1095:1127	Oligosaccharide mapping, or merely complete disaccharide composition, may be performed on nanogram-scale by the fluorescence detector vs micrograms useful for classical SAX-HPLC.
27062721	7	27	theme	heparin	1528:1534	arg1	chains					1536:1541	heparin chains	1528:1541	heparin chains	1528:1541	Contrary to SAX-HPLC, this novel HILIC approach is able to separate and identify the saturated trisulfated disaccharide belonging to the non-reducing end of heparin chains.
27062721	8	28	theme	region	1606:1611	arg1	region					1606:1611	the ATIII-binding region	1588:1611	the ATIII-binding region	1588:1611	Finally, the content of 3-O-sulfo groups of the ATIII-binding region is determined.
27062721	8	28	theme	region	1606:1611	arg1	groups					1578:1583	3-O-sulfo groups	1568:1583	3-O-sulfo groups of the ATIII-binding region	1568:1611	Finally, the content of 3-O-sulfo groups of the ATIII-binding region is determined.
27062721	0	29	theme	heparinase	27:36	arg1	heparins					48:55	heparinase I-treated heparins	27:55	heparinase I-treated heparins	27:55	Oligosaccharide mapping of heparinase I-treated heparins by hydrophilic interaction liquid chromatography separation and online fluorescence detection and electrospray ionization-mass spectrometry characterization.
27062721	8	30	theme	ATIII-binding	1592:1604	arg1	region					1606:1611	the ATIII-binding region	1588:1611	the ATIII-binding region	1588:1611	Finally, the content of 3-O-sulfo groups of the ATIII-binding region is determined.
27062721	0	31	theme	electrospray	155:166	arg1	spectrometry					184:195	electrospray ionization-mass spectrometry	155:195	electrospray ionization-mass spectrometry	155:195	Oligosaccharide mapping of heparinase I-treated heparins by hydrophilic interaction liquid chromatography separation and online fluorescence detection and electrospray ionization-mass spectrometry characterization.
27062721	3	32	theme	oligosaccharide	649:663	arg1	strategy					673:680	a novel oligosaccharide mapping strategy	641:680	a novel oligosaccharide mapping strategy based on the HILIC separation of the main heparin disaccharides/oligosaccharides released by heparinase I, fluorotagged with 2-aminoacridone and on-line detected by a fluorescence detector and characterized by ESI-MS	641:897	We report a novel oligosaccharide mapping strategy based on the HILIC separation of the main heparin disaccharides/oligosaccharides released by heparinase I, fluorotagged with 2-aminoacridone and on-line detected by a fluorescence detector and characterized by ESI-MS.
27062721	4	33	theme	high	944:947	arg1	pH					949:950	a high pH	942:950	a high pH with acetonitrile	942:968	The application of a polar solvent having a high pH with acetonitrile avoided desulfation enabling a simple and accurate structural oligosaccharide assignment.
27062721	7	34	theme	saturated	1456:1464	arg1	disaccharide					1478:1489	the saturated trisulfated disaccharide	1452:1489	the saturated trisulfated disaccharide belonging to the non-reducing end of heparin chains	1452:1541	Contrary to SAX-HPLC, this novel HILIC approach is able to separate and identify the saturated trisulfated disaccharide belonging to the non-reducing end of heparin chains.
27062721	2	35	theme	-HPLC	504:508	arg1	separation					510:519	strong-anion exchange (SAX)-HPLC separation	477:519	strong-anion exchange (SAX)-HPLC separation which is incompatible with MS requiring purification of oligosaccharides for their conclusive identification	477:628	This approach is performed by strong-anion exchange (SAX)-HPLC separation which is incompatible with MS requiring purification of oligosaccharides for their conclusive identification.
27062721	3	36	theme	mapping	665:671	arg1	strategy					673:680	a novel oligosaccharide mapping strategy	641:680	a novel oligosaccharide mapping strategy based on the HILIC separation of the main heparin disaccharides/oligosaccharides released by heparinase I, fluorotagged with 2-aminoacridone and on-line detected by a fluorescence detector and characterized by ESI-MS	641:897	We report a novel oligosaccharide mapping strategy based on the HILIC separation of the main heparin disaccharides/oligosaccharides released by heparinase I, fluorotagged with 2-aminoacridone and on-line detected by a fluorescence detector and characterized by ESI-MS.
27062721	2	37	theme	SAX	500:502	arg1	separation					510:519	strong-anion exchange (SAX)-HPLC separation	477:519	strong-anion exchange (SAX)-HPLC separation which is incompatible with MS requiring purification of oligosaccharides for their conclusive identification	477:628	This approach is performed by strong-anion exchange (SAX)-HPLC separation which is incompatible with MS requiring purification of oligosaccharides for their conclusive identification.
27062721	0	38	theme	heparins	48:55	arg1	spectrometry					184:195	electrospray ionization-mass spectrometry	155:195	electrospray ionization-mass spectrometry	155:195	Oligosaccharide mapping of heparinase I-treated heparins by hydrophilic interaction liquid chromatography separation and online fluorescence detection and electrospray ionization-mass spectrometry characterization.
27062721	0	38	theme	heparins	48:55	arg1	mapping					16:22	Oligosaccharide mapping	0:22	Oligosaccharide mapping of heparinase I-treated heparins by hydrophilic interaction liquid chromatography separation	0:115	Oligosaccharide mapping of heparinase I-treated heparins by hydrophilic interaction liquid chromatography separation and online fluorescence detection and electrospray ionization-mass spectrometry characterization.
27062721	0	38	theme	heparins	48:55	arg1	detection					141:149	online fluorescence detection	121:149	online fluorescence detection	121:149	Oligosaccharide mapping of heparinase I-treated heparins by hydrophilic interaction liquid chromatography separation and online fluorescence detection and electrospray ionization-mass spectrometry characterization.
27062721	3	39	theme	heparinase	775:784	arg1	I					786:786	heparinase I	775:786	heparinase I	775:786	We report a novel oligosaccharide mapping strategy based on the HILIC separation of the main heparin disaccharides/oligosaccharides released by heparinase I, fluorotagged with 2-aminoacridone and on-line detected by a fluorescence detector and characterized by ESI-MS.
27062721	3	40	theme	HILIC	695:699	arg1	separation					701:710	the HILIC separation	691:710	the HILIC separation of the main heparin disaccharides/oligosaccharides released by heparinase I, fluorotagged with 2-aminoacridone and on-line detected by a fluorescence detector and characterized by ESI-MS	691:897	We report a novel oligosaccharide mapping strategy based on the HILIC separation of the main heparin disaccharides/oligosaccharides released by heparinase I, fluorotagged with 2-aminoacridone and on-line detected by a fluorescence detector and characterized by ESI-MS.
27062721	1	41	theme	useful	275:280	arg1	fingerprint					292:302	a useful molecular fingerprint	273:302	a useful molecular fingerprint of the heparin structure revealing detailed structural information regarding its sequence and the content of part of the ATIII-binding region	273:444	Oligosaccharide mapping based on enzyme cleavage provides a useful molecular fingerprint of the heparin structure revealing detailed structural information regarding its sequence and the content of part of the ATIII-binding region.
27062721	0	42	theme	I-treated	38:46	arg1	heparins					48:55	heparinase I-treated heparins	27:55	heparinase I-treated heparins	27:55	Oligosaccharide mapping of heparinase I-treated heparins by hydrophilic interaction liquid chromatography separation and online fluorescence detection and electrospray ionization-mass spectrometry characterization.
27062721	0	43	theme	ionization-mass	168:182	arg1	spectrometry					184:195	electrospray ionization-mass spectrometry	155:195	electrospray ionization-mass spectrometry	155:195	Oligosaccharide mapping of heparinase I-treated heparins by hydrophilic interaction liquid chromatography separation and online fluorescence detection and electrospray ionization-mass spectrometry characterization.
27062721	3	44	theme	disaccharides/oligosaccharides	732:761	arg1	separation					701:710	the HILIC separation	691:710	the HILIC separation of the main heparin disaccharides/oligosaccharides released by heparinase I, fluorotagged with 2-aminoacridone and on-line detected by a fluorescence detector and characterized by ESI-MS	691:897	We report a novel oligosaccharide mapping strategy based on the HILIC separation of the main heparin disaccharides/oligosaccharides released by heparinase I, fluorotagged with 2-aminoacridone and on-line detected by a fluorescence detector and characterized by ESI-MS.
27062721	6	45	dep	heparinases	1347:1357	arg1	heparinases					1347:1357	expensive heparinases II and III	1337:1368	expensive heparinases II and III	1337:1368	Additionally, only widely commercially available heparin lyase I is necessary, without the use of expensive heparinases II and III.
27062721	6	45	dep	heparinases	1347:1357	arg1	III					1366:1368	III	1366:1368	III	1366:1368	Additionally, only widely commercially available heparin lyase I is necessary, without the use of expensive heparinases II and III.
27062721	6	45	dep	heparinases	1347:1357	arg1	II					1359:1360	II	1359:1360	II	1359:1360	Additionally, only widely commercially available heparin lyase I is necessary, without the use of expensive heparinases II and III.
27062721	1	46	theme	molecular	282:290	arg1	fingerprint					292:302	a useful molecular fingerprint	273:302	a useful molecular fingerprint of the heparin structure revealing detailed structural information regarding its sequence and the content of part of the ATIII-binding region	273:444	Oligosaccharide mapping based on enzyme cleavage provides a useful molecular fingerprint of the heparin structure revealing detailed structural information regarding its sequence and the content of part of the ATIII-binding region.
27062721	0	47	theme	hydrophilic	60:70	arg1	chromatography					91:104	hydrophilic interaction liquid chromatography	60:104	hydrophilic interaction liquid chromatography separation	60:115	Oligosaccharide mapping of heparinase I-treated heparins by hydrophilic interaction liquid chromatography separation and online fluorescence detection and electrospray ionization-mass spectrometry characterization.
27062721	4	48	theme	oligosaccharide	1032:1046	arg1	assignment					1048:1057	a simple and accurate structural oligosaccharide assignment	999:1057	a simple and accurate structural oligosaccharide assignment	999:1057	The application of a polar solvent having a high pH with acetonitrile avoided desulfation enabling a simple and accurate structural oligosaccharide assignment.
27062721	2	49	theme	oligosaccharides	577:592	arg1	purification					561:572	purification	561:572	purification of oligosaccharides	561:592	This approach is performed by strong-anion exchange (SAX)-HPLC separation which is incompatible with MS requiring purification of oligosaccharides for their conclusive identification.
27062721	7	50	theme	chains	1536:1541	arg1	end					1521:1523	the non-reducing end	1504:1523	the non-reducing end of heparin chains	1504:1541	Contrary to SAX-HPLC, this novel HILIC approach is able to separate and identify the saturated trisulfated disaccharide belonging to the non-reducing end of heparin chains.
27062721	5	51	theme	Oligosaccharide	1060:1074	arg1	mapping					1076:1082	Oligosaccharide mapping	1060:1082	Oligosaccharide mapping	1060:1082	Oligosaccharide mapping, or merely complete disaccharide composition, may be performed on nanogram-scale by the fluorescence detector vs micrograms useful for classical SAX-HPLC.
27062721	4	52	theme	structural	1021:1030	arg1	assignment					1048:1057	a simple and accurate structural oligosaccharide assignment	999:1057	a simple and accurate structural oligosaccharide assignment	999:1057	The application of a polar solvent having a high pH with acetonitrile avoided desulfation enabling a simple and accurate structural oligosaccharide assignment.
27062721	5	53	theme	useful	1208:1213	arg1	micrograms					1197:1206	micrograms	1197:1206	micrograms useful for classical SAX-HPLC	1197:1236	Oligosaccharide mapping, or merely complete disaccharide composition, may be performed on nanogram-scale by the fluorescence detector vs micrograms useful for classical SAX-HPLC.
27062721	4	54	theme	polar	921:925	arg1	solvent					927:933	a polar solvent	919:933	a polar solvent having a high pH with acetonitrile	919:968	The application of a polar solvent having a high pH with acetonitrile avoided desulfation enabling a simple and accurate structural oligosaccharide assignment.
27062721	3	55	theme	main	719:722	arg1	disaccharides/oligosaccharides					732:761	the main heparin disaccharides/oligosaccharides	715:761	the main heparin disaccharides/oligosaccharides released by heparinase I, fluorotagged with 2-aminoacridone and on-line detected by a fluorescence detector and characterized by ESI-MS	715:897	We report a novel oligosaccharide mapping strategy based on the HILIC separation of the main heparin disaccharides/oligosaccharides released by heparinase I, fluorotagged with 2-aminoacridone and on-line detected by a fluorescence detector and characterized by ESI-MS.
27062721	5	56	theme	disaccharide	1104:1115	arg1	composition					1117:1127	complete disaccharide composition	1095:1127	complete disaccharide composition	1095:1127	Oligosaccharide mapping, or merely complete disaccharide composition, may be performed on nanogram-scale by the fluorescence detector vs micrograms useful for classical SAX-HPLC.
27062721	3	57	dep	disaccharides/oligosaccharides	732:761	arg1	fluorotagged					789:800	fluorotagged	789:800	fluorotagged with 2-aminoacridone and on-line detected by a fluorescence detector	789:869	We report a novel oligosaccharide mapping strategy based on the HILIC separation of the main heparin disaccharides/oligosaccharides released by heparinase I, fluorotagged with 2-aminoacridone and on-line detected by a fluorescence detector and characterized by ESI-MS.
27062721	3	57	dep	disaccharides/oligosaccharides	732:761	arg1	released					763:770	released	763:770	released by heparinase I	763:786	We report a novel oligosaccharide mapping strategy based on the HILIC separation of the main heparin disaccharides/oligosaccharides released by heparinase I, fluorotagged with 2-aminoacridone and on-line detected by a fluorescence detector and characterized by ESI-MS.
27062721	3	57	dep	disaccharides/oligosaccharides	732:761	arg1	characterized					875:887	characterized	875:887	characterized by ESI-MS	875:897	We report a novel oligosaccharide mapping strategy based on the HILIC separation of the main heparin disaccharides/oligosaccharides released by heparinase I, fluorotagged with 2-aminoacridone and on-line detected by a fluorescence detector and characterized by ESI-MS.
27062721	5	58	theme	classical	1219:1227	arg1	SAX-HPLC					1229:1236	classical SAX-HPLC	1219:1236	classical SAX-HPLC	1219:1236	Oligosaccharide mapping, or merely complete disaccharide composition, may be performed on nanogram-scale by the fluorescence detector vs micrograms useful for classical SAX-HPLC.
27062721	3	59	theme	heparin	724:730	arg1	disaccharides/oligosaccharides					732:761	the main heparin disaccharides/oligosaccharides	715:761	the main heparin disaccharides/oligosaccharides released by heparinase I, fluorotagged with 2-aminoacridone and on-line detected by a fluorescence detector and characterized by ESI-MS	715:897	We report a novel oligosaccharide mapping strategy based on the HILIC separation of the main heparin disaccharides/oligosaccharides released by heparinase I, fluorotagged with 2-aminoacridone and on-line detected by a fluorescence detector and characterized by ESI-MS.
27062721	4	60	contain	having	935:940	arg1	solvent					927:933	a polar solvent	919:933	a polar solvent having a high pH with acetonitrile	919:968	The application of a polar solvent having a high pH with acetonitrile avoided desulfation enabling a simple and accurate structural oligosaccharide assignment.
27062721	4	60	contain	having	935:940	arg2	pH					949:950	a high pH	942:950	a high pH with acetonitrile	942:968	The application of a polar solvent having a high pH with acetonitrile avoided desulfation enabling a simple and accurate structural oligosaccharide assignment.
27062721	0	61	theme	liquid	84:89	arg1	chromatography					91:104	hydrophilic interaction liquid chromatography	60:104	hydrophilic interaction liquid chromatography separation	60:115	Oligosaccharide mapping of heparinase I-treated heparins by hydrophilic interaction liquid chromatography separation and online fluorescence detection and electrospray ionization-mass spectrometry characterization.
27062721	2	62	theme	exchange	490:497	arg1	separation					510:519	strong-anion exchange (SAX)-HPLC separation	477:519	strong-anion exchange (SAX)-HPLC separation which is incompatible with MS requiring purification of oligosaccharides for their conclusive identification	477:628	This approach is performed by strong-anion exchange (SAX)-HPLC separation which is incompatible with MS requiring purification of oligosaccharides for their conclusive identification.
27062721	7	63	theme	non-reducing	1508:1519	arg1	end					1521:1523	the non-reducing end	1504:1523	the non-reducing end of heparin chains	1504:1541	Contrary to SAX-HPLC, this novel HILIC approach is able to separate and identify the saturated trisulfated disaccharide belonging to the non-reducing end of heparin chains.
27062721	2	64	with	incompatible	530:541	arg1	MS					548:549	MS	548:549	MS requiring purification of oligosaccharides for their conclusive identification	548:628	This approach is performed by strong-anion exchange (SAX)-HPLC separation which is incompatible with MS requiring purification of oligosaccharides for their conclusive identification.
27062721	6	65	theme	heparin	1288:1294	arg1	lyase					1296:1300	only widely commercially available heparin lyase I	1253:1302	only widely commercially available heparin lyase I	1253:1302	Additionally, only widely commercially available heparin lyase I is necessary, without the use of expensive heparinases II and III.
27062721	0	66	theme	interaction	72:82	arg1	chromatography					91:104	hydrophilic interaction liquid chromatography	60:104	hydrophilic interaction liquid chromatography separation	60:115	Oligosaccharide mapping of heparinase I-treated heparins by hydrophilic interaction liquid chromatography separation and online fluorescence detection and electrospray ionization-mass spectrometry characterization.
27062721	1	67	theme	part	413:416	arg1	sequence					385:392	its sequence	381:392	its sequence	381:392	Oligosaccharide mapping based on enzyme cleavage provides a useful molecular fingerprint of the heparin structure revealing detailed structural information regarding its sequence and the content of part of the ATIII-binding region.
27062721	1	67	theme	part	413:416	arg1	content					402:408	the content	398:408	the content of part of the ATIII-binding region	398:444	Oligosaccharide mapping based on enzyme cleavage provides a useful molecular fingerprint of the heparin structure revealing detailed structural information regarding its sequence and the content of part of the ATIII-binding region.
27062721	4	68	theme	solvent	927:933	arg1	application					904:914	The application	900:914	The application of a polar solvent having a high pH with acetonitrile	900:968	The application of a polar solvent having a high pH with acetonitrile avoided desulfation enabling a simple and accurate structural oligosaccharide assignment.
27062721	6	69	theme	available	1278:1286	arg1	lyase					1296:1300	only widely commercially available heparin lyase I	1253:1302	only widely commercially available heparin lyase I	1253:1302	Additionally, only widely commercially available heparin lyase I is necessary, without the use of expensive heparinases II and III.
27062721	7	70	theme	novel	1398:1402	arg1	approach					1410:1417	this novel HILIC approach	1393:1417	this novel HILIC approach	1393:1417	Contrary to SAX-HPLC, this novel HILIC approach is able to separate and identify the saturated trisulfated disaccharide belonging to the non-reducing end of heparin chains.
27062721	1	71	theme	heparin	311:317	arg1	structure					319:327	the heparin structure	307:327	the heparin structure revealing detailed structural information regarding its sequence and the content of part of the ATIII-binding region	307:444	Oligosaccharide mapping based on enzyme cleavage provides a useful molecular fingerprint of the heparin structure revealing detailed structural information regarding its sequence and the content of part of the ATIII-binding region.
26686110	0	0	theme	composite	97:105	arg1	matrix					107:112	tamarind seed gum composite matrix	79:112	tamarind seed gum composite matrix	79:112	Gastroretentive extended release of metformin from methacrylamide-g-gellan and tamarind seed gum composite matrix.
26686110	4	1	theme	XRD	669:671	arg1	analysis					673:680	XRD analysis	669:680	XRD analysis	669:680	Compatibility between drug and excipients was checked by DSC, FTIR and XRD analysis.
26686110	1	2	theme	metformin	175:183	arg1	tablet					165:170	a gastroretentive extended release tablet	130:170	a gastroretentive extended release tablet of metformin based on polymethacrylamide-g-gellan (Pmaa-g-GG)-tamarind seed gum (TSG) composite matrix	130:273	Formulation of a gastroretentive extended release tablet of metformin based on polymethacrylamide-g-gellan (Pmaa-g-GG)-tamarind seed gum (TSG) composite matrix is the main purpose of this study.
26686110	0	3	theme	gum	93:95	arg1	matrix					107:112	tamarind seed gum composite matrix	79:112	tamarind seed gum composite matrix	79:112	Gastroretentive extended release of metformin from methacrylamide-g-gellan and tamarind seed gum composite matrix.
26686110	7	4	theme	anomalous	1142:1150	arg1	mechanism					1193:1201	anomalous non-Fickian transport based drug release mechanism	1142:1201	anomalous non-Fickian transport based drug release mechanism	1142:1201	Kinetic modeling unveiled anomalous non-Fickian transport based drug release mechanism.
26686110	3	5	theme	in	510:511	arg1	release					524:530	in vitro drug release	510:530	in vitro drug release	510:530	The tablets were then evaluated for in vitro drug release, buoyancy, ex vivo mucoadhesion, swelling and surface morphology.
26686110	6	6	theme	lag	1017:1019	arg1	time					1021:1024	buoyancy lag time	1008:1024	buoyancy lag time of 2.76min, significant mucoadhesion and drug release	1008:1078	The optimized formulation showed excellent buoyancy over a 10h period with buoyancy lag time of 2.76min, significant mucoadhesion and drug release over a period of 10h with f2=71.58.
26686110	7	7	theme	Kinetic	1116:1122	arg1	modeling					1124:1131	Kinetic modeling	1116:1131	Kinetic modeling	1116:1131	Kinetic modeling unveiled anomalous non-Fickian transport based drug release mechanism.
26686110	6	8	theme	buoyancy	1008:1015	arg1	time					1021:1024	buoyancy lag time	1008:1024	buoyancy lag time of 2.76min, significant mucoadhesion and drug release	1008:1078	The optimized formulation showed excellent buoyancy over a 10h period with buoyancy lag time of 2.76min, significant mucoadhesion and drug release over a period of 10h with f2=71.58.
26686110	5	9	theme	%	725:725	arg1	release					732:738	% drug release	725:738	% drug release	725:738	Buoyancy-lag-time, mucoadhesive strength, % drug release and release-rate constant were statistically analyzed using Design-Expert software (version 9.0.4.1) and the formulation was then numerically optimized to obtain USP-reference release profile.
26686110	5	10	theme	USP-reference	902:914	arg1	profile					924:930	USP-reference release profile	902:930	USP-reference release profile	902:930	Buoyancy-lag-time, mucoadhesive strength, % drug release and release-rate constant were statistically analyzed using Design-Expert software (version 9.0.4.1) and the formulation was then numerically optimized to obtain USP-reference release profile.
26686110	1	11	theme	main	282:285	arg1	purpose					287:293	the main purpose	278:293	the main purpose of this study	278:307	Formulation of a gastroretentive extended release tablet of metformin based on polymethacrylamide-g-gellan (Pmaa-g-GG)-tamarind seed gum (TSG) composite matrix is the main purpose of this study.
26686110	1	11	theme	main	282:285	arg1	Formulation					115:125	Formulation	115:125	Formulation of a gastroretentive extended release tablet of metformin based on polymethacrylamide-g-gellan (Pmaa-g-GG)-tamarind seed gum (TSG) composite matrix	115:273	Formulation of a gastroretentive extended release tablet of metformin based on polymethacrylamide-g-gellan (Pmaa-g-GG)-tamarind seed gum (TSG) composite matrix is the main purpose of this study.
26686110	5	12	theme	drug	727:730	arg1	release					732:738	% drug release	725:738	% drug release	725:738	Buoyancy-lag-time, mucoadhesive strength, % drug release and release-rate constant were statistically analyzed using Design-Expert software (version 9.0.4.1) and the formulation was then numerically optimized to obtain USP-reference release profile.
26686110	0	13	from	methacrylamide-g-gellan	51:73	arg1	release					25:31	Gastroretentive extended release	0:31	Gastroretentive extended release of metformin from methacrylamide-g-gellan and tamarind seed gum composite matrix.	0:113	Gastroretentive extended release of metformin from methacrylamide-g-gellan and tamarind seed gum composite matrix.
26686110	6	14	theme	drug	1067:1070	arg1	release					1072:1078	drug release	1067:1078	drug release	1067:1078	The optimized formulation showed excellent buoyancy over a 10h period with buoyancy lag time of 2.76min, significant mucoadhesion and drug release over a period of 10h with f2=71.58.
26686110	2	15	dep	Pmaa-g-GG	382:390	arg1	SBC					409:411	SBC	409:411	SBC	409:411	Tablets were prepared employing wet granulation method taking amount of Pmaa-g-GG, TSG and NaHCO3 (SBC, buoyancy contributor) as independent formulation variables.
26686110	2	15	dep	Pmaa-g-GG	382:390	arg1	contributor					423:433	buoyancy contributor	414:433	buoyancy contributor	414:433	Tablets were prepared employing wet granulation method taking amount of Pmaa-g-GG, TSG and NaHCO3 (SBC, buoyancy contributor) as independent formulation variables.
26686110	2	16	theme	NaHCO3	401:406	arg1	variables					463:471	independent formulation variables	439:471	independent formulation variables	439:471	Tablets were prepared employing wet granulation method taking amount of Pmaa-g-GG, TSG and NaHCO3 (SBC, buoyancy contributor) as independent formulation variables.
26686110	2	16	theme	NaHCO3	401:406	arg1	amount					372:377	amount	372:377	amount of Pmaa-g-GG, TSG and NaHCO3 (SBC, buoyancy contributor)	372:434	Tablets were prepared employing wet granulation method taking amount of Pmaa-g-GG, TSG and NaHCO3 (SBC, buoyancy contributor) as independent formulation variables.
26686110	2	16	theme	NaHCO3	401:406	arg1	NaHCO3					401:406	NaHCO3	401:406	NaHCO3	401:406	Tablets were prepared employing wet granulation method taking amount of Pmaa-g-GG, TSG and NaHCO3 (SBC, buoyancy contributor) as independent formulation variables.
26686110	2	16	theme	NaHCO3	401:406	arg1	TSG					393:395	TSG	393:395	TSG	393:395	Tablets were prepared employing wet granulation method taking amount of Pmaa-g-GG, TSG and NaHCO3 (SBC, buoyancy contributor) as independent formulation variables.
26686110	2	16	theme	NaHCO3	401:406	arg1	Pmaa-g-GG					382:390	Pmaa-g-GG	382:390	Pmaa-g-GG	382:390	Tablets were prepared employing wet granulation method taking amount of Pmaa-g-GG, TSG and NaHCO3 (SBC, buoyancy contributor) as independent formulation variables.
26686110	5	17	theme	Design-Expert	800:812	arg1	version					824:830	version 9.0.4.1	824:838	version 9.0.4.1	824:838	Buoyancy-lag-time, mucoadhesive strength, % drug release and release-rate constant were statistically analyzed using Design-Expert software (version 9.0.4.1) and the formulation was then numerically optimized to obtain USP-reference release profile.
26686110	5	17	theme	Design-Expert	800:812	arg1	software					814:821	Design-Expert software	800:821	Design-Expert software (version 9.0.4.1)	800:839	Buoyancy-lag-time, mucoadhesive strength, % drug release and release-rate constant were statistically analyzed using Design-Expert software (version 9.0.4.1) and the formulation was then numerically optimized to obtain USP-reference release profile.
26686110	6	18	theme	10h	1097:1099	arg1	period					1087:1092	a period	1085:1092	a period of 10h with f2=71.58	1085:1113	The optimized formulation showed excellent buoyancy over a 10h period with buoyancy lag time of 2.76min, significant mucoadhesion and drug release over a period of 10h with f2=71.58.
26686110	1	19	theme	polymethacrylamide-g-gellan	194:220	arg1	TSG					253:255	TSG	253:255	TSG	253:255	Formulation of a gastroretentive extended release tablet of metformin based on polymethacrylamide-g-gellan (Pmaa-g-GG)-tamarind seed gum (TSG) composite matrix is the main purpose of this study.
26686110	1	19	theme	polymethacrylamide-g-gellan	194:220	arg1	gum					248:250	polymethacrylamide-g-gellan (Pmaa-g-GG)-tamarind seed gum	194:250	polymethacrylamide-g-gellan (Pmaa-g-GG)-tamarind seed gum (TSG) composite matrix	194:273	Formulation of a gastroretentive extended release tablet of metformin based on polymethacrylamide-g-gellan (Pmaa-g-GG)-tamarind seed gum (TSG) composite matrix is the main purpose of this study.
26686110	0	20	theme	extended	16:23	arg1	release					25:31	Gastroretentive extended release	0:31	Gastroretentive extended release of metformin from methacrylamide-g-gellan and tamarind seed gum composite matrix.	0:113	Gastroretentive extended release of metformin from methacrylamide-g-gellan and tamarind seed gum composite matrix.
26686110	3	21	theme	drug	519:522	arg1	release					524:530	in vitro drug release	510:530	in vitro drug release	510:530	The tablets were then evaluated for in vitro drug release, buoyancy, ex vivo mucoadhesion, swelling and surface morphology.
26686110	6	22	with	period	996:1001	arg1	time					1021:1024	buoyancy lag time	1008:1024	buoyancy lag time of 2.76min, significant mucoadhesion and drug release	1008:1078	The optimized formulation showed excellent buoyancy over a 10h period with buoyancy lag time of 2.76min, significant mucoadhesion and drug release over a period of 10h with f2=71.58.
26686110	6	23	with	10h	1097:1099	arg1	f2=71.58					1106:1113	f2=71.58	1106:1113	f2=71.58	1106:1113	The optimized formulation showed excellent buoyancy over a 10h period with buoyancy lag time of 2.76min, significant mucoadhesion and drug release over a period of 10h with f2=71.58.
26686110	2	24	theme	TSG	393:395	arg1	variables					463:471	independent formulation variables	439:471	independent formulation variables	439:471	Tablets were prepared employing wet granulation method taking amount of Pmaa-g-GG, TSG and NaHCO3 (SBC, buoyancy contributor) as independent formulation variables.
26686110	2	24	theme	TSG	393:395	arg1	amount					372:377	amount	372:377	amount of Pmaa-g-GG, TSG and NaHCO3 (SBC, buoyancy contributor)	372:434	Tablets were prepared employing wet granulation method taking amount of Pmaa-g-GG, TSG and NaHCO3 (SBC, buoyancy contributor) as independent formulation variables.
26686110	2	24	theme	TSG	393:395	arg1	NaHCO3					401:406	NaHCO3	401:406	NaHCO3	401:406	Tablets were prepared employing wet granulation method taking amount of Pmaa-g-GG, TSG and NaHCO3 (SBC, buoyancy contributor) as independent formulation variables.
26686110	2	24	theme	TSG	393:395	arg1	TSG					393:395	TSG	393:395	TSG	393:395	Tablets were prepared employing wet granulation method taking amount of Pmaa-g-GG, TSG and NaHCO3 (SBC, buoyancy contributor) as independent formulation variables.
26686110	2	24	theme	TSG	393:395	arg1	Pmaa-g-GG					382:390	Pmaa-g-GG	382:390	Pmaa-g-GG	382:390	Tablets were prepared employing wet granulation method taking amount of Pmaa-g-GG, TSG and NaHCO3 (SBC, buoyancy contributor) as independent formulation variables.
26686110	0	25	theme	Gastroretentive	0:14	arg1	release					25:31	Gastroretentive extended release	0:31	Gastroretentive extended release of metformin from methacrylamide-g-gellan and tamarind seed gum composite matrix.	0:113	Gastroretentive extended release of metformin from methacrylamide-g-gellan and tamarind seed gum composite matrix.
26686110	3	26	dep	in	510:511	arg1	vitro					513:517	vitro	513:517	vitro	513:517	The tablets were then evaluated for in vitro drug release, buoyancy, ex vivo mucoadhesion, swelling and surface morphology.
26686110	1	27	theme	Pmaa-g-GG	223:231	arg1	TSG					253:255	TSG	253:255	TSG	253:255	Formulation of a gastroretentive extended release tablet of metformin based on polymethacrylamide-g-gellan (Pmaa-g-GG)-tamarind seed gum (TSG) composite matrix is the main purpose of this study.
26686110	1	27	theme	Pmaa-g-GG	223:231	arg1	gum					248:250	polymethacrylamide-g-gellan (Pmaa-g-GG)-tamarind seed gum	194:250	polymethacrylamide-g-gellan (Pmaa-g-GG)-tamarind seed gum (TSG) composite matrix	194:273	Formulation of a gastroretentive extended release tablet of metformin based on polymethacrylamide-g-gellan (Pmaa-g-GG)-tamarind seed gum (TSG) composite matrix is the main purpose of this study.
26686110	7	28	theme	release	1185:1191	arg1	mechanism					1193:1201	anomalous non-Fickian transport based drug release mechanism	1142:1201	anomalous non-Fickian transport based drug release mechanism	1142:1201	Kinetic modeling unveiled anomalous non-Fickian transport based drug release mechanism.
26686110	6	29	theme	10h	992:994	arg1	period					996:1001	a 10h period	990:1001	a 10h period with buoyancy lag time of 2.76min, significant mucoadhesion and drug release	990:1078	The optimized formulation showed excellent buoyancy over a 10h period with buoyancy lag time of 2.76min, significant mucoadhesion and drug release over a period of 10h with f2=71.58.
26686110	3	30	theme	ex	543:544	arg1	mucoadhesion					551:562	ex vivo mucoadhesion	543:562	ex vivo mucoadhesion	543:562	The tablets were then evaluated for in vitro drug release, buoyancy, ex vivo mucoadhesion, swelling and surface morphology.
26686110	2	31	theme	buoyancy	414:421	arg1	SBC					409:411	SBC	409:411	SBC	409:411	Tablets were prepared employing wet granulation method taking amount of Pmaa-g-GG, TSG and NaHCO3 (SBC, buoyancy contributor) as independent formulation variables.
26686110	2	31	theme	buoyancy	414:421	arg1	contributor					423:433	buoyancy contributor	414:433	buoyancy contributor	414:433	Tablets were prepared employing wet granulation method taking amount of Pmaa-g-GG, TSG and NaHCO3 (SBC, buoyancy contributor) as independent formulation variables.
26686110	2	32	theme	Pmaa-g-GG	382:390	arg1	variables					463:471	independent formulation variables	439:471	independent formulation variables	439:471	Tablets were prepared employing wet granulation method taking amount of Pmaa-g-GG, TSG and NaHCO3 (SBC, buoyancy contributor) as independent formulation variables.
26686110	2	32	theme	Pmaa-g-GG	382:390	arg1	amount					372:377	amount	372:377	amount of Pmaa-g-GG, TSG and NaHCO3 (SBC, buoyancy contributor)	372:434	Tablets were prepared employing wet granulation method taking amount of Pmaa-g-GG, TSG and NaHCO3 (SBC, buoyancy contributor) as independent formulation variables.
26686110	2	32	theme	Pmaa-g-GG	382:390	arg1	NaHCO3					401:406	NaHCO3	401:406	NaHCO3	401:406	Tablets were prepared employing wet granulation method taking amount of Pmaa-g-GG, TSG and NaHCO3 (SBC, buoyancy contributor) as independent formulation variables.
26686110	2	32	theme	Pmaa-g-GG	382:390	arg1	TSG					393:395	TSG	393:395	TSG	393:395	Tablets were prepared employing wet granulation method taking amount of Pmaa-g-GG, TSG and NaHCO3 (SBC, buoyancy contributor) as independent formulation variables.
26686110	2	32	theme	Pmaa-g-GG	382:390	arg1	Pmaa-g-GG					382:390	Pmaa-g-GG	382:390	Pmaa-g-GG	382:390	Tablets were prepared employing wet granulation method taking amount of Pmaa-g-GG, TSG and NaHCO3 (SBC, buoyancy contributor) as independent formulation variables.
26686110	5	33	theme	release	916:922	arg1	profile					924:930	USP-reference release profile	902:930	USP-reference release profile	902:930	Buoyancy-lag-time, mucoadhesive strength, % drug release and release-rate constant were statistically analyzed using Design-Expert software (version 9.0.4.1) and the formulation was then numerically optimized to obtain USP-reference release profile.
26686110	1	34	theme	-tamarind	233:241	arg1	TSG					253:255	TSG	253:255	TSG	253:255	Formulation of a gastroretentive extended release tablet of metformin based on polymethacrylamide-g-gellan (Pmaa-g-GG)-tamarind seed gum (TSG) composite matrix is the main purpose of this study.
26686110	1	34	theme	-tamarind	233:241	arg1	gum					248:250	polymethacrylamide-g-gellan (Pmaa-g-GG)-tamarind seed gum	194:250	polymethacrylamide-g-gellan (Pmaa-g-GG)-tamarind seed gum (TSG) composite matrix	194:273	Formulation of a gastroretentive extended release tablet of metformin based on polymethacrylamide-g-gellan (Pmaa-g-GG)-tamarind seed gum (TSG) composite matrix is the main purpose of this study.
26686110	7	35	theme	based	1174:1178	arg1	mechanism					1193:1201	anomalous non-Fickian transport based drug release mechanism	1142:1201	anomalous non-Fickian transport based drug release mechanism	1142:1201	Kinetic modeling unveiled anomalous non-Fickian transport based drug release mechanism.
26686110	1	36	theme	study	303:307	arg1	purpose					287:293	the main purpose	278:293	the main purpose of this study	278:307	Formulation of a gastroretentive extended release tablet of metformin based on polymethacrylamide-g-gellan (Pmaa-g-GG)-tamarind seed gum (TSG) composite matrix is the main purpose of this study.
26686110	1	36	theme	study	303:307	arg1	Formulation					115:125	Formulation	115:125	Formulation of a gastroretentive extended release tablet of metformin based on polymethacrylamide-g-gellan (Pmaa-g-GG)-tamarind seed gum (TSG) composite matrix	115:273	Formulation of a gastroretentive extended release tablet of metformin based on polymethacrylamide-g-gellan (Pmaa-g-GG)-tamarind seed gum (TSG) composite matrix is the main purpose of this study.
26686110	5	37	theme	mucoadhesive	702:713	arg1	strength					715:722	mucoadhesive strength	702:722	mucoadhesive strength	702:722	Buoyancy-lag-time, mucoadhesive strength, % drug release and release-rate constant were statistically analyzed using Design-Expert software (version 9.0.4.1) and the formulation was then numerically optimized to obtain USP-reference release profile.
26686110	3	38	theme	surface	578:584	arg1	morphology					586:595	surface morphology	578:595	surface morphology	578:595	The tablets were then evaluated for in vitro drug release, buoyancy, ex vivo mucoadhesion, swelling and surface morphology.
26686110	1	39	theme	seed	243:246	arg1	TSG					253:255	TSG	253:255	TSG	253:255	Formulation of a gastroretentive extended release tablet of metformin based on polymethacrylamide-g-gellan (Pmaa-g-GG)-tamarind seed gum (TSG) composite matrix is the main purpose of this study.
26686110	1	39	theme	seed	243:246	arg1	gum					248:250	polymethacrylamide-g-gellan (Pmaa-g-GG)-tamarind seed gum	194:250	polymethacrylamide-g-gellan (Pmaa-g-GG)-tamarind seed gum (TSG) composite matrix	194:273	Formulation of a gastroretentive extended release tablet of metformin based on polymethacrylamide-g-gellan (Pmaa-g-GG)-tamarind seed gum (TSG) composite matrix is the main purpose of this study.
26686110	0	40	theme	metformin	36:44	arg1	release					25:31	Gastroretentive extended release	0:31	Gastroretentive extended release of metformin from methacrylamide-g-gellan and tamarind seed gum composite matrix.	0:113	Gastroretentive extended release of metformin from methacrylamide-g-gellan and tamarind seed gum composite matrix.
26686110	7	41	theme	drug	1180:1183	arg1	mechanism					1193:1201	anomalous non-Fickian transport based drug release mechanism	1142:1201	anomalous non-Fickian transport based drug release mechanism	1142:1201	Kinetic modeling unveiled anomalous non-Fickian transport based drug release mechanism.
26686110	6	42	theme	2.76min	1029:1035	arg1	time					1021:1024	buoyancy lag time	1008:1024	buoyancy lag time of 2.76min, significant mucoadhesion and drug release	1008:1078	The optimized formulation showed excellent buoyancy over a 10h period with buoyancy lag time of 2.76min, significant mucoadhesion and drug release over a period of 10h with f2=71.58.
26686110	1	43	theme	gum	248:250	arg1	matrix					268:273	polymethacrylamide-g-gellan (Pmaa-g-GG)-tamarind seed gum (TSG) composite matrix	194:273	polymethacrylamide-g-gellan (Pmaa-g-GG)-tamarind seed gum (TSG) composite matrix	194:273	Formulation of a gastroretentive extended release tablet of metformin based on polymethacrylamide-g-gellan (Pmaa-g-GG)-tamarind seed gum (TSG) composite matrix is the main purpose of this study.
26686110	7	44	theme	non-Fickian	1152:1162	arg1	mechanism					1193:1201	anomalous non-Fickian transport based drug release mechanism	1142:1201	anomalous non-Fickian transport based drug release mechanism	1142:1201	Kinetic modeling unveiled anomalous non-Fickian transport based drug release mechanism.
26686110	6	45	theme	excellent	966:974	arg1	buoyancy					976:983	excellent buoyancy	966:983	excellent buoyancy	966:983	The optimized formulation showed excellent buoyancy over a 10h period with buoyancy lag time of 2.76min, significant mucoadhesion and drug release over a period of 10h with f2=71.58.
26686110	6	46	theme	mucoadhesion	1050:1061	arg1	time					1021:1024	buoyancy lag time	1008:1024	buoyancy lag time of 2.76min, significant mucoadhesion and drug release	1008:1078	The optimized formulation showed excellent buoyancy over a 10h period with buoyancy lag time of 2.76min, significant mucoadhesion and drug release over a period of 10h with f2=71.58.
26686110	5	47	theme	release-rate	744:755	arg1	constant					757:764	release-rate constant	744:764	release-rate constant	744:764	Buoyancy-lag-time, mucoadhesive strength, % drug release and release-rate constant were statistically analyzed using Design-Expert software (version 9.0.4.1) and the formulation was then numerically optimized to obtain USP-reference release profile.
26686110	2	48	theme	formulation	451:461	arg1	variables					463:471	independent formulation variables	439:471	independent formulation variables	439:471	Tablets were prepared employing wet granulation method taking amount of Pmaa-g-GG, TSG and NaHCO3 (SBC, buoyancy contributor) as independent formulation variables.
26686110	2	48	theme	formulation	451:461	arg1	Pmaa-g-GG					382:390	Pmaa-g-GG	382:390	Pmaa-g-GG	382:390	Tablets were prepared employing wet granulation method taking amount of Pmaa-g-GG, TSG and NaHCO3 (SBC, buoyancy contributor) as independent formulation variables.
26686110	2	48	theme	formulation	451:461	arg1	NaHCO3					401:406	NaHCO3	401:406	NaHCO3	401:406	Tablets were prepared employing wet granulation method taking amount of Pmaa-g-GG, TSG and NaHCO3 (SBC, buoyancy contributor) as independent formulation variables.
26686110	2	48	theme	formulation	451:461	arg1	TSG					393:395	TSG	393:395	TSG	393:395	Tablets were prepared employing wet granulation method taking amount of Pmaa-g-GG, TSG and NaHCO3 (SBC, buoyancy contributor) as independent formulation variables.
26686110	2	48	theme	formulation	451:461	arg1	amount					372:377	amount	372:377	amount of Pmaa-g-GG, TSG and NaHCO3 (SBC, buoyancy contributor)	372:434	Tablets were prepared employing wet granulation method taking amount of Pmaa-g-GG, TSG and NaHCO3 (SBC, buoyancy contributor) as independent formulation variables.
26686110	1	49	theme	gastroretentive	132:146	arg1	tablet					165:170	a gastroretentive extended release tablet	130:170	a gastroretentive extended release tablet of metformin based on polymethacrylamide-g-gellan (Pmaa-g-GG)-tamarind seed gum (TSG) composite matrix	130:273	Formulation of a gastroretentive extended release tablet of metformin based on polymethacrylamide-g-gellan (Pmaa-g-GG)-tamarind seed gum (TSG) composite matrix is the main purpose of this study.
26686110	7	50	theme	transport	1164:1172	arg1	mechanism					1193:1201	anomalous non-Fickian transport based drug release mechanism	1142:1201	anomalous non-Fickian transport based drug release mechanism	1142:1201	Kinetic modeling unveiled anomalous non-Fickian transport based drug release mechanism.
26686110	1	51	theme	extended	148:155	arg1	tablet					165:170	a gastroretentive extended release tablet	130:170	a gastroretentive extended release tablet of metformin based on polymethacrylamide-g-gellan (Pmaa-g-GG)-tamarind seed gum (TSG) composite matrix	130:273	Formulation of a gastroretentive extended release tablet of metformin based on polymethacrylamide-g-gellan (Pmaa-g-GG)-tamarind seed gum (TSG) composite matrix is the main purpose of this study.
26686110	6	52	theme	significant	1038:1048	arg1	mucoadhesion					1050:1061	significant mucoadhesion	1038:1061	significant mucoadhesion	1038:1061	The optimized formulation showed excellent buoyancy over a 10h period with buoyancy lag time of 2.76min, significant mucoadhesion and drug release over a period of 10h with f2=71.58.
26686110	6	53	theme	release	1072:1078	arg1	time					1021:1024	buoyancy lag time	1008:1024	buoyancy lag time of 2.76min, significant mucoadhesion and drug release	1008:1078	The optimized formulation showed excellent buoyancy over a 10h period with buoyancy lag time of 2.76min, significant mucoadhesion and drug release over a period of 10h with f2=71.58.
26686110	1	54	theme	release	157:163	arg1	tablet					165:170	a gastroretentive extended release tablet	130:170	a gastroretentive extended release tablet of metformin based on polymethacrylamide-g-gellan (Pmaa-g-GG)-tamarind seed gum (TSG) composite matrix	130:273	Formulation of a gastroretentive extended release tablet of metformin based on polymethacrylamide-g-gellan (Pmaa-g-GG)-tamarind seed gum (TSG) composite matrix is the main purpose of this study.
26686110	2	55	theme	wet	342:344	arg1	method					358:363	wet granulation method	342:363	wet granulation method taking amount of Pmaa-g-GG, TSG and NaHCO3 (SBC, buoyancy contributor) as independent formulation variables	342:471	Tablets were prepared employing wet granulation method taking amount of Pmaa-g-GG, TSG and NaHCO3 (SBC, buoyancy contributor) as independent formulation variables.
26686110	0	56	theme	seed	88:91	arg1	matrix					107:112	tamarind seed gum composite matrix	79:112	tamarind seed gum composite matrix	79:112	Gastroretentive extended release of metformin from methacrylamide-g-gellan and tamarind seed gum composite matrix.
26686110	0	57	from	matrix	107:112	arg1	release					25:31	Gastroretentive extended release	0:31	Gastroretentive extended release of metformin from methacrylamide-g-gellan and tamarind seed gum composite matrix.	0:113	Gastroretentive extended release of metformin from methacrylamide-g-gellan and tamarind seed gum composite matrix.
26686110	3	58	dep	ex	543:544	arg1	vivo					546:549	vivo	546:549	vivo	546:549	The tablets were then evaluated for in vitro drug release, buoyancy, ex vivo mucoadhesion, swelling and surface morphology.
26686110	1	59	theme	tablet	165:170	arg1	purpose					287:293	the main purpose	278:293	the main purpose of this study	278:307	Formulation of a gastroretentive extended release tablet of metformin based on polymethacrylamide-g-gellan (Pmaa-g-GG)-tamarind seed gum (TSG) composite matrix is the main purpose of this study.
26686110	1	59	theme	tablet	165:170	arg1	Formulation					115:125	Formulation	115:125	Formulation of a gastroretentive extended release tablet of metformin based on polymethacrylamide-g-gellan (Pmaa-g-GG)-tamarind seed gum (TSG) composite matrix	115:273	Formulation of a gastroretentive extended release tablet of metformin based on polymethacrylamide-g-gellan (Pmaa-g-GG)-tamarind seed gum (TSG) composite matrix is the main purpose of this study.
26686110	2	60	theme	granulation	346:356	arg1	method					358:363	wet granulation method	342:363	wet granulation method taking amount of Pmaa-g-GG, TSG and NaHCO3 (SBC, buoyancy contributor) as independent formulation variables	342:471	Tablets were prepared employing wet granulation method taking amount of Pmaa-g-GG, TSG and NaHCO3 (SBC, buoyancy contributor) as independent formulation variables.
26686110	0	61	theme	tamarind	79:86	arg1	matrix					107:112	tamarind seed gum composite matrix	79:112	tamarind seed gum composite matrix	79:112	Gastroretentive extended release of metformin from methacrylamide-g-gellan and tamarind seed gum composite matrix.
26686110	6	62	theme	optimized	937:945	arg1	formulation					947:957	The optimized formulation	933:957	The optimized formulation	933:957	The optimized formulation showed excellent buoyancy over a 10h period with buoyancy lag time of 2.76min, significant mucoadhesion and drug release over a period of 10h with f2=71.58.
26686110	2	63	theme	independent	439:449	arg1	variables					463:471	independent formulation variables	439:471	independent formulation variables	439:471	Tablets were prepared employing wet granulation method taking amount of Pmaa-g-GG, TSG and NaHCO3 (SBC, buoyancy contributor) as independent formulation variables.
26686110	2	63	theme	independent	439:449	arg1	Pmaa-g-GG					382:390	Pmaa-g-GG	382:390	Pmaa-g-GG	382:390	Tablets were prepared employing wet granulation method taking amount of Pmaa-g-GG, TSG and NaHCO3 (SBC, buoyancy contributor) as independent formulation variables.
26686110	2	63	theme	independent	439:449	arg1	NaHCO3					401:406	NaHCO3	401:406	NaHCO3	401:406	Tablets were prepared employing wet granulation method taking amount of Pmaa-g-GG, TSG and NaHCO3 (SBC, buoyancy contributor) as independent formulation variables.
26686110	2	63	theme	independent	439:449	arg1	TSG					393:395	TSG	393:395	TSG	393:395	Tablets were prepared employing wet granulation method taking amount of Pmaa-g-GG, TSG and NaHCO3 (SBC, buoyancy contributor) as independent formulation variables.
26686110	2	63	theme	independent	439:449	arg1	amount					372:377	amount	372:377	amount of Pmaa-g-GG, TSG and NaHCO3 (SBC, buoyancy contributor)	372:434	Tablets were prepared employing wet granulation method taking amount of Pmaa-g-GG, TSG and NaHCO3 (SBC, buoyancy contributor) as independent formulation variables.
26686110	1	64	theme	composite	258:266	arg1	matrix					268:273	polymethacrylamide-g-gellan (Pmaa-g-GG)-tamarind seed gum (TSG) composite matrix	194:273	polymethacrylamide-g-gellan (Pmaa-g-GG)-tamarind seed gum (TSG) composite matrix	194:273	Formulation of a gastroretentive extended release tablet of metformin based on polymethacrylamide-g-gellan (Pmaa-g-GG)-tamarind seed gum (TSG) composite matrix is the main purpose of this study.
28900693	5	0	theme	genomic	739:745	arg1	content					750:756	an average genomic GC content	728:756	an average genomic GC content of 48.7%	728:765	It has a total of 3.676 genes with an average genomic GC content of 48.7%.
28900693	8	1	theme	utilization	1004:1014	arg1	locus					1022:1026	The hemicellulose utilization (HUS) locus	986:1026	The hemicellulose utilization (HUS) locus	986:1026	The hemicellulose utilization (HUS) locus was identified based on sequence homology against G. stearothermophilus T-6.
28900693	2	2	theme	tractable	358:366	arg1	It					302:303	It	302:303	It	302:303	It was selected from over 500 isolates as a genetically tractable hemicellulolytic lactic acid producer, requiring little nutrients.
28900693	2	2	theme	tractable	358:366	arg1	producer					397:404	a genetically tractable hemicellulolytic lactic acid producer	344:404	a genetically tractable hemicellulolytic lactic acid producer	344:404	It was selected from over 500 isolates as a genetically tractable hemicellulolytic lactic acid producer, requiring little nutrients.
28900693	5	3	theme	GC	747:748	arg1	content					750:756	an average genomic GC content	728:756	an average genomic GC content of 48.7%	728:765	It has a total of 3.676 genes with an average genomic GC content of 48.7%.
28900693	8	4	theme	hemicellulose	990:1002	arg1	locus					1022:1026	The hemicellulose utilization (HUS) locus	986:1026	The hemicellulose utilization (HUS) locus	986:1026	The hemicellulose utilization (HUS) locus was identified based on sequence homology against G. stearothermophilus T-6.
28900693	13	5	theme	thermodenitrificans	1626:1644	arg1	candidate					1679:1687	a potentially interesting candidate	1653:1687	a potentially interesting candidate for industrial applications	1653:1715	It is concluded that G. thermodenitrificans T12 is a potentially interesting candidate for industrial applications.
28900693	13	5	theme	thermodenitrificans	1626:1644	arg1	T12					1646:1648	G. thermodenitrificans T12	1623:1648	G. thermodenitrificans T12	1623:1648	It is concluded that G. thermodenitrificans T12 is a potentially interesting candidate for industrial applications.
28900693	4	6	theme	56 kb	686:690	arg1	chromosome					648:657	a 3.64 Mb chromosome	638:657	a 3.64 Mb chromosome	638:657	The genome of strain T12 consists of a 3.64 Mb chromosome and two plasmids of 59 and 56 kb.
28900693	4	6	theme	56 kb	686:690	arg1	plasmids					667:674	two plasmids	663:674	two plasmids of 59 and 56 kb	663:690	The genome of strain T12 consists of a 3.64 Mb chromosome and two plasmids of 59 and 56 kb.
28900693	11	7	theme	several	1428:1434	arg1	vitamins					1436:1443	several vitamins	1428:1443	several vitamins	1428:1443	Strain T12 has complete pathways for the synthesis of purine and pyrimidine, all 20 amino acids and several vitamins except D-biotin.
28900693	11	7	theme	several	1428:1434	arg1	pathways					1352:1359	complete pathways	1343:1359	complete pathways for the synthesis of purine and pyrimidine	1343:1402	Strain T12 has complete pathways for the synthesis of purine and pyrimidine, all 20 amino acids and several vitamins except D-biotin.
28900693	8	8	theme	HUS	1017:1019	arg1	locus					1022:1026	The hemicellulose utilization (HUS) locus	986:1026	The hemicellulose utilization (HUS) locus	986:1026	The hemicellulose utilization (HUS) locus was identified based on sequence homology against G. stearothermophilus T-6.
28900693	6	9	theme	T12	772:774	arg1	pathway					809:815	a denitrification pathway	791:815	a denitrification pathway	791:815	The T12 genome encodes a denitrification pathway, allowing for anaerobic respiration.
28900693	6	9	theme	T12	772:774	arg1	genome					776:781	The T12 genome	768:781	The T12 genome	768:781	The T12 genome encodes a denitrification pathway, allowing for anaerobic respiration.
28900693	0	10	theme	Biotechnological	86:101	arg1	Applications					103:114	Biotechnological Applications	86:114	Biotechnological Applications	86:114	Complete Genome Sequence of Geobacillus thermodenitrificans T12, A Potential Host for Biotechnological Applications.
28900693	11	11	contain	has	1339:1341	arg2	pathways					1352:1359	complete pathways	1343:1359	complete pathways for the synthesis of purine and pyrimidine	1343:1402	Strain T12 has complete pathways for the synthesis of purine and pyrimidine, all 20 amino acids and several vitamins except D-biotin.
28900693	11	11	contain	has	1339:1341	arg1	T12					1335:1337	Strain T12	1328:1337	Strain T12	1328:1337	Strain T12 has complete pathways for the synthesis of purine and pyrimidine, all 20 amino acids and several vitamins except D-biotin.
28900693	11	11	contain	has	1339:1341	arg2	vitamins					1436:1443	several vitamins	1428:1443	several vitamins	1428:1443	Strain T12 has complete pathways for the synthesis of purine and pyrimidine, all 20 amino acids and several vitamins except D-biotin.
28900693	11	11	contain	has	1339:1341	arg2	acids					1418:1422	all 20 amino acids	1405:1422	all 20 amino acids	1405:1422	Strain T12 has complete pathways for the synthesis of purine and pyrimidine, all 20 amino acids and several vitamins except D-biotin.
28900693	3	12	theme	lactic	515:520	arg1	acid					522:525	lactic acid	515:525	lactic acid from xylan	515:536	The strain is able to ferment glucose and xylose simultaneously and can produce lactic acid from xylan, making it a potential host for biotechnological applications.
28900693	12	13	theme	Type	1586:1589	arg1	system					1594:1599	a CRISPR-Cas Type II system	1573:1599	a CRISPR-Cas Type II system	1573:1599	The host-defense systems present comprise a Type II and a Type III restriction-modification system, as well as a CRISPR-Cas Type II system.
28900693	9	14	from	strain	1164:1169	arg1	genes					1138:1142	all the genes	1130:1142	all the genes that are present in strain T-6 except for the arabinan degradation cluster	1130:1217	It appeared that T12 has all the genes that are present in strain T-6 except for the arabinan degradation cluster.
28900693	9	14	from	strain	1164:1169	arg1	present					1153:1159	present	1153:1159	present	1153:1159	It appeared that T12 has all the genes that are present in strain T-6 except for the arabinan degradation cluster.
28900693	5	15	theme	genes	717:721	arg1	total					702:706	a total	700:706	a total of 3.676 genes	700:721	It has a total of 3.676 genes with an average genomic GC content of 48.7%.
28900693	9	16	attach	present	1153:1159	arg2	genes					1138:1142	all the genes	1130:1142	all the genes that are present in strain T-6 except for the arabinan degradation cluster	1130:1217	It appeared that T12 has all the genes that are present in strain T-6 except for the arabinan degradation cluster.
28900693	9	16	attach	present	1153:1159	arg2	present					1153:1159	present	1153:1159	present	1153:1159	It appeared that T12 has all the genes that are present in strain T-6 except for the arabinan degradation cluster.
28900693	9	16	attach	present	1153:1159	arg1	strain					1164:1169	strain T-6	1164:1173	strain T-6	1164:1173	It appeared that T12 has all the genes that are present in strain T-6 except for the arabinan degradation cluster.
28900693	3	17	from	xylan	532:536	arg1	acid					522:525	lactic acid	515:525	lactic acid from xylan	515:536	The strain is able to ferment glucose and xylose simultaneously and can produce lactic acid from xylan, making it a potential host for biotechnological applications.
28900693	12	18	theme	Type	1506:1509	arg1	system					1554:1559	a Type II and a Type III restriction-modification system	1504:1559	a Type II and a Type III restriction-modification system	1504:1559	The host-defense systems present comprise a Type II and a Type III restriction-modification system, as well as a CRISPR-Cas Type II system.
28900693	11	19	theme	purine	1382:1387	arg1	synthesis					1369:1377	the synthesis	1365:1377	the synthesis of purine and pyrimidine	1365:1402	Strain T12 has complete pathways for the synthesis of purine and pyrimidine, all 20 amino acids and several vitamins except D-biotin.
28900693	11	20	theme	complete	1343:1350	arg1	acids					1418:1422	all 20 amino acids	1405:1422	all 20 amino acids	1405:1422	Strain T12 has complete pathways for the synthesis of purine and pyrimidine, all 20 amino acids and several vitamins except D-biotin.
28900693	11	20	theme	complete	1343:1350	arg1	vitamins					1436:1443	several vitamins	1428:1443	several vitamins	1428:1443	Strain T12 has complete pathways for the synthesis of purine and pyrimidine, all 20 amino acids and several vitamins except D-biotin.
28900693	11	20	theme	complete	1343:1350	arg1	pathways					1352:1359	complete pathways	1343:1359	complete pathways for the synthesis of purine and pyrimidine	1343:1402	Strain T12 has complete pathways for the synthesis of purine and pyrimidine, all 20 amino acids and several vitamins except D-biotin.
28900693	10	21	contain	contains	1257:1264	arg1	locus					1237:1241	the HUS locus	1229:1241	the HUS locus of strain T12	1229:1255	Instead, the HUS locus of strain T12 contains genes for both an inositol and a pectate degradation pathway.
28900693	10	21	contain	contains	1257:1264	arg2	genes					1266:1270	genes	1266:1270	genes for both an inositol and a pectate degradation pathway	1266:1325	Instead, the HUS locus of strain T12 contains genes for both an inositol and a pectate degradation pathway.
28900693	1	22	link	derived	195:201	arg1	substrates					203:212	lignocellulose derived substrates	180:212	lignocellulose derived substrates	180:212	In attempt to obtain a thermophilic host for the conversion of lignocellulose derived substrates into lactic acid, Geobacillus thermodenitrificans T12 was isolated from a compost heap.
28900693	12	23	theme	present	1487:1493	arg1	systems					1479:1485	The host-defense systems	1462:1485	The host-defense systems present	1462:1493	The host-defense systems present comprise a Type II and a Type III restriction-modification system, as well as a CRISPR-Cas Type II system.
28900693	10	24	theme	HUS	1233:1235	arg1	locus					1237:1241	the HUS locus	1229:1241	the HUS locus of strain T12	1229:1255	Instead, the HUS locus of strain T12 contains genes for both an inositol and a pectate degradation pathway.
28900693	12	25	theme	Type	1520:1523	arg1	system					1554:1559	a Type II and a Type III restriction-modification system	1504:1559	a Type II and a Type III restriction-modification system	1504:1559	The host-defense systems present comprise a Type II and a Type III restriction-modification system, as well as a CRISPR-Cas Type II system.
28900693	0	26	theme	Genome	9:14	arg1	Sequence					16:23	Complete Genome Sequence	0:23	Complete Genome Sequence of Geobacillus	0:38	Complete Genome Sequence of Geobacillus thermodenitrificans T12, A Potential Host for Biotechnological Applications.
28900693	11	27	theme	Strain	1328:1333	arg1	T12					1335:1337	Strain T12	1328:1337	Strain T12	1328:1337	Strain T12 has complete pathways for the synthesis of purine and pyrimidine, all 20 amino acids and several vitamins except D-biotin.
28900693	13	28	theme	interesting	1667:1677	arg1	candidate					1679:1687	a potentially interesting candidate	1653:1687	a potentially interesting candidate for industrial applications	1653:1715	It is concluded that G. thermodenitrificans T12 is a potentially interesting candidate for industrial applications.
28900693	13	28	theme	interesting	1667:1677	arg1	T12					1646:1648	G. thermodenitrificans T12	1623:1648	G. thermodenitrificans T12	1623:1648	It is concluded that G. thermodenitrificans T12 is a potentially interesting candidate for industrial applications.
28900693	0	29	theme	Complete	0:7	arg1	Sequence					16:23	Complete Genome Sequence	0:23	Complete Genome Sequence of Geobacillus	0:38	Complete Genome Sequence of Geobacillus thermodenitrificans T12, A Potential Host for Biotechnological Applications.
28900693	1	30	theme	compost	288:294	arg1	heap					296:299	a compost heap	286:299	a compost heap	286:299	In attempt to obtain a thermophilic host for the conversion of lignocellulose derived substrates into lactic acid, Geobacillus thermodenitrificans T12 was isolated from a compost heap.
28900693	7	31	theme	responsible	891:901	arg1	genes					903:907	the responsible genes	887:907	the responsible genes	887:907	The identity and localization of the responsible genes are similar to those of the denitrification pathways found in strain NG80-2.
28900693	3	32	theme	biotechnological	570:585	arg1	applications					587:598	biotechnological applications	570:598	biotechnological applications	570:598	The strain is able to ferment glucose and xylose simultaneously and can produce lactic acid from xylan, making it a potential host for biotechnological applications.
28900693	2	33	theme	acid	392:395	arg1	It					302:303	It	302:303	It	302:303	It was selected from over 500 isolates as a genetically tractable hemicellulolytic lactic acid producer, requiring little nutrients.
28900693	2	33	theme	acid	392:395	arg1	producer					397:404	a genetically tractable hemicellulolytic lactic acid producer	344:404	a genetically tractable hemicellulolytic lactic acid producer	344:404	It was selected from over 500 isolates as a genetically tractable hemicellulolytic lactic acid producer, requiring little nutrients.
28900693	13	34	theme	industrial	1693:1702	arg1	applications					1704:1715	industrial applications	1693:1715	industrial applications	1693:1715	It is concluded that G. thermodenitrificans T12 is a potentially interesting candidate for industrial applications.
28900693	7	35	theme	genes	903:907	arg1	localization					871:882	localization	871:882	localization	871:882	The identity and localization of the responsible genes are similar to those of the denitrification pathways found in strain NG80-2.
28900693	7	35	theme	genes	903:907	arg1	identity					858:865	identity	858:865	identity	858:865	The identity and localization of the responsible genes are similar to those of the denitrification pathways found in strain NG80-2.
28900693	3	36	theme	potential	551:559	arg1	host					561:564	a potential host	549:564	a potential host for biotechnological applications	549:598	The strain is able to ferment glucose and xylose simultaneously and can produce lactic acid from xylan, making it a potential host for biotechnological applications.
28900693	1	37	theme	substrates	203:212	arg1	conversion					166:175	the conversion	162:175	the conversion of lignocellulose derived substrates into lactic acid	162:229	In attempt to obtain a thermophilic host for the conversion of lignocellulose derived substrates into lactic acid, Geobacillus thermodenitrificans T12 was isolated from a compost heap.
28900693	5	38	contain	has	696:698	arg2	total					702:706	a total	700:706	a total of 3.676 genes	700:721	It has a total of 3.676 genes with an average genomic GC content of 48.7%.
28900693	5	38	contain	has	696:698	arg1	It					693:694	It	693:694	It	693:694	It has a total of 3.676 genes with an average genomic GC content of 48.7%.
28900693	6	39	theme	anaerobic	831:839	arg1	respiration					841:851	anaerobic respiration	831:851	anaerobic respiration	831:851	The T12 genome encodes a denitrification pathway, allowing for anaerobic respiration.
28900693	10	40	theme	strain	1246:1251	arg1	T12					1253:1255	strain T12	1246:1255	strain T12	1246:1255	Instead, the HUS locus of strain T12 contains genes for both an inositol and a pectate degradation pathway.
28900693	10	41	theme	T12	1253:1255	arg1	locus					1237:1241	the HUS locus	1229:1241	the HUS locus of strain T12	1229:1255	Instead, the HUS locus of strain T12 contains genes for both an inositol and a pectate degradation pathway.
28900693	1	42	theme	thermophilic	140:151	arg1	host					153:156	a thermophilic host	138:156	a thermophilic host for the conversion of lignocellulose derived substrates into lactic acid	138:229	In attempt to obtain a thermophilic host for the conversion of lignocellulose derived substrates into lactic acid, Geobacillus thermodenitrificans T12 was isolated from a compost heap.
28900693	11	43	theme	pyrimidine	1393:1402	arg1	synthesis					1369:1377	the synthesis	1365:1377	the synthesis of purine and pyrimidine	1365:1402	Strain T12 has complete pathways for the synthesis of purine and pyrimidine, all 20 amino acids and several vitamins except D-biotin.
28900693	12	44	theme	CRISPR-Cas	1575:1584	arg1	system					1594:1599	a CRISPR-Cas Type II system	1573:1599	a CRISPR-Cas Type II system	1573:1599	The host-defense systems present comprise a Type II and a Type III restriction-modification system, as well as a CRISPR-Cas Type II system.
28900693	1	45	theme	derived	195:201	arg1	substrates					203:212	lignocellulose derived substrates	180:212	lignocellulose derived substrates	180:212	In attempt to obtain a thermophilic host for the conversion of lignocellulose derived substrates into lactic acid, Geobacillus thermodenitrificans T12 was isolated from a compost heap.
28900693	1	46	theme	lactic	219:224	arg1	acid					226:229	lactic acid	219:229	lactic acid	219:229	In attempt to obtain a thermophilic host for the conversion of lignocellulose derived substrates into lactic acid, Geobacillus thermodenitrificans T12 was isolated from a compost heap.
28900693	0	47	theme	Geobacillus	28:38	arg1	Sequence					16:23	Complete Genome Sequence	0:23	Complete Genome Sequence of Geobacillus	0:38	Complete Genome Sequence of Geobacillus thermodenitrificans T12, A Potential Host for Biotechnological Applications.
28900693	8	48	theme	sequence	1052:1059	arg1	homology					1061:1068	sequence homology	1052:1068	sequence homology against G. stearothermophilus T-6	1052:1102	The hemicellulose utilization (HUS) locus was identified based on sequence homology against G. stearothermophilus T-6.
28900693	5	49	theme	3.676	711:715	arg1	genes					717:721	3.676 genes	711:721	3.676 genes	711:721	It has a total of 3.676 genes with an average genomic GC content of 48.7%.
28900693	1	50	dep	derived	195:201	arg1	lignocellulose					180:193	lignocellulose	180:193	lignocellulose	180:193	In attempt to obtain a thermophilic host for the conversion of lignocellulose derived substrates into lactic acid, Geobacillus thermodenitrificans T12 was isolated from a compost heap.
28900693	2	51	theme	little	417:422	arg1	nutrients					424:432	little nutrients	417:432	little nutrients	417:432	It was selected from over 500 isolates as a genetically tractable hemicellulolytic lactic acid producer, requiring little nutrients.
28900693	7	52	located	found	962:966	arg2	pathways					953:960	the denitrification pathways	933:960	the denitrification pathways found in strain NG80-2	933:983	The identity and localization of the responsible genes are similar to those of the denitrification pathways found in strain NG80-2.
28900693	7	52	located	found	962:966	arg1	strain					971:976	strain NG80-2	971:983	strain NG80-2	971:983	The identity and localization of the responsible genes are similar to those of the denitrification pathways found in strain NG80-2.
28900693	9	53	located	present	1153:1159	arg1	strain					1164:1169	strain T-6	1164:1173	strain T-6	1164:1173	It appeared that T12 has all the genes that are present in strain T-6 except for the arabinan degradation cluster.
28900693	9	53	located	present	1153:1159	arg2	present					1153:1159	present	1153:1159	present	1153:1159	It appeared that T12 has all the genes that are present in strain T-6 except for the arabinan degradation cluster.
28900693	9	53	located	present	1153:1159	arg2	genes					1138:1142	all the genes	1130:1142	all the genes that are present in strain T-6 except for the arabinan degradation cluster	1130:1217	It appeared that T12 has all the genes that are present in strain T-6 except for the arabinan degradation cluster.
28900693	5	54	theme	%	765:765	arg1	content					750:756	an average genomic GC content	728:756	an average genomic GC content of 48.7%	728:765	It has a total of 3.676 genes with an average genomic GC content of 48.7%.
28900693	9	55	contain	has	1126:1128	arg2	genes					1138:1142	all the genes	1130:1142	all the genes that are present in strain T-6 except for the arabinan degradation cluster	1130:1217	It appeared that T12 has all the genes that are present in strain T-6 except for the arabinan degradation cluster.
28900693	9	55	contain	has	1126:1128	arg2	present					1153:1159	present	1153:1159	present	1153:1159	It appeared that T12 has all the genes that are present in strain T-6 except for the arabinan degradation cluster.
28900693	9	55	contain	has	1126:1128	arg1	T12					1122:1124	T12	1122:1124	T12	1122:1124	It appeared that T12 has all the genes that are present in strain T-6 except for the arabinan degradation cluster.
28900693	12	56	dep	Type	1506:1509	arg1	a					1504:1504	a	1504:1504	a	1504:1504	The host-defense systems present comprise a Type II and a Type III restriction-modification system, as well as a CRISPR-Cas Type II system.
28900693	9	57	theme	degradation	1199:1209	arg1	cluster					1211:1217	the arabinan degradation cluster	1186:1217	the arabinan degradation cluster	1186:1217	It appeared that T12 has all the genes that are present in strain T-6 except for the arabinan degradation cluster.
28900693	9	58	theme	arabinan	1190:1197	arg1	cluster					1211:1217	the arabinan degradation cluster	1186:1217	the arabinan degradation cluster	1186:1217	It appeared that T12 has all the genes that are present in strain T-6 except for the arabinan degradation cluster.
28900693	7	59	dep	identity	858:865	arg1	The					854:856	The	854:856	The	854:856	The identity and localization of the responsible genes are similar to those of the denitrification pathways found in strain NG80-2.
28900693	6	60	theme	denitrification	793:807	arg1	pathway					809:815	a denitrification pathway	791:815	a denitrification pathway	791:815	The T12 genome encodes a denitrification pathway, allowing for anaerobic respiration.
28900693	6	60	theme	denitrification	793:807	arg1	genome					776:781	The T12 genome	768:781	The T12 genome	768:781	The T12 genome encodes a denitrification pathway, allowing for anaerobic respiration.
28900693	1	61	attach	isolated	272:279	arg2	T12					264:266	Geobacillus thermodenitrificans T12	232:266	Geobacillus thermodenitrificans T12	232:266	In attempt to obtain a thermophilic host for the conversion of lignocellulose derived substrates into lactic acid, Geobacillus thermodenitrificans T12 was isolated from a compost heap.
28900693	1	61	attach	isolated	272:279	arg1	heap					296:299	a compost heap	286:299	a compost heap	286:299	In attempt to obtain a thermophilic host for the conversion of lignocellulose derived substrates into lactic acid, Geobacillus thermodenitrificans T12 was isolated from a compost heap.
28900693	9	62	from	present	1153:1159	arg1	strain					1164:1169	strain T-6	1164:1173	strain T-6	1164:1173	It appeared that T12 has all the genes that are present in strain T-6 except for the arabinan degradation cluster.
28900693	4	63	theme	strain	615:620	arg1	T12					622:624	strain T12	615:624	strain T12	615:624	The genome of strain T12 consists of a 3.64 Mb chromosome and two plasmids of 59 and 56 kb.
28900693	12	64	theme	restriction-modification	1529:1552	arg1	system					1554:1559	a Type II and a Type III restriction-modification system	1504:1559	a Type II and a Type III restriction-modification system	1504:1559	The host-defense systems present comprise a Type II and a Type III restriction-modification system, as well as a CRISPR-Cas Type II system.
28900693	10	65	theme	degradation	1307:1317	arg1	pathway					1319:1325	both an inositol and a pectate degradation pathway	1276:1325	pathway	1319:1325	Instead, the HUS locus of strain T12 contains genes for both an inositol and a pectate degradation pathway.
28900693	4	66	theme	59	679:680	arg1	chromosome					648:657	a 3.64 Mb chromosome	638:657	a 3.64 Mb chromosome	638:657	The genome of strain T12 consists of a 3.64 Mb chromosome and two plasmids of 59 and 56 kb.
28900693	4	66	theme	59	679:680	arg1	plasmids					667:674	two plasmids	663:674	two plasmids of 59 and 56 kb	663:690	The genome of strain T12 consists of a 3.64 Mb chromosome and two plasmids of 59 and 56 kb.
28900693	13	67	theme	G.	1623:1624	arg1	candidate					1679:1687	a potentially interesting candidate	1653:1687	a potentially interesting candidate for industrial applications	1653:1715	It is concluded that G. thermodenitrificans T12 is a potentially interesting candidate for industrial applications.
28900693	13	67	theme	G.	1623:1624	arg1	T12					1646:1648	G. thermodenitrificans T12	1623:1648	G. thermodenitrificans T12	1623:1648	It is concluded that G. thermodenitrificans T12 is a potentially interesting candidate for industrial applications.
28900693	1	68	theme	Geobacillus	232:242	arg1	T12					264:266	Geobacillus thermodenitrificans T12	232:266	Geobacillus thermodenitrificans T12	232:266	In attempt to obtain a thermophilic host for the conversion of lignocellulose derived substrates into lactic acid, Geobacillus thermodenitrificans T12 was isolated from a compost heap.
28900693	0	69	theme	Potential	67:75	arg1	Host					77:80	A Potential Host	65:80	A Potential Host for Biotechnological Applications	65:114	Complete Genome Sequence of Geobacillus thermodenitrificans T12, A Potential Host for Biotechnological Applications.
28900693	0	69	theme	Potential	67:75	arg1	T12					60:62	T12	60:62	T12	60:62	Complete Genome Sequence of Geobacillus thermodenitrificans T12, A Potential Host for Biotechnological Applications.
28900693	4	70	theme	3.64 Mb	640:646	arg1	chromosome					648:657	a 3.64 Mb chromosome	638:657	a 3.64 Mb chromosome	638:657	The genome of strain T12 consists of a 3.64 Mb chromosome and two plasmids of 59 and 56 kb.
28900693	12	71	theme	host-defense	1466:1477	arg1	systems					1479:1485	The host-defense systems	1462:1485	The host-defense systems present	1462:1493	The host-defense systems present comprise a Type II and a Type III restriction-modification system, as well as a CRISPR-Cas Type II system.
28900693	10	72	theme	pectate	1299:1305	arg1	pathway					1319:1325	both an inositol and a pectate degradation pathway	1276:1325	pathway	1319:1325	Instead, the HUS locus of strain T12 contains genes for both an inositol and a pectate degradation pathway.
28900693	2	73	theme	lactic	385:390	arg1	It					302:303	It	302:303	It	302:303	It was selected from over 500 isolates as a genetically tractable hemicellulolytic lactic acid producer, requiring little nutrients.
28900693	2	73	theme	lactic	385:390	arg1	producer					397:404	a genetically tractable hemicellulolytic lactic acid producer	344:404	a genetically tractable hemicellulolytic lactic acid producer	344:404	It was selected from over 500 isolates as a genetically tractable hemicellulolytic lactic acid producer, requiring little nutrients.
28900693	11	74	theme	amino	1412:1416	arg1	acids					1418:1422	all 20 amino acids	1405:1422	all 20 amino acids	1405:1422	Strain T12 has complete pathways for the synthesis of purine and pyrimidine, all 20 amino acids and several vitamins except D-biotin.
28900693	11	74	theme	amino	1412:1416	arg1	pathways					1352:1359	complete pathways	1343:1359	complete pathways for the synthesis of purine and pyrimidine	1343:1402	Strain T12 has complete pathways for the synthesis of purine and pyrimidine, all 20 amino acids and several vitamins except D-biotin.
28900693	1	75	theme	thermodenitrificans	244:262	arg1	T12					264:266	Geobacillus thermodenitrificans T12	232:266	Geobacillus thermodenitrificans T12	232:266	In attempt to obtain a thermophilic host for the conversion of lignocellulose derived substrates into lactic acid, Geobacillus thermodenitrificans T12 was isolated from a compost heap.
28900693	5	76	theme	average	731:737	arg1	content					750:756	an average genomic GC content	728:756	an average genomic GC content of 48.7%	728:765	It has a total of 3.676 genes with an average genomic GC content of 48.7%.
28900693	7	77	theme	denitrification	937:951	arg1	pathways					953:960	the denitrification pathways	933:960	the denitrification pathways found in strain NG80-2	933:983	The identity and localization of the responsible genes are similar to those of the denitrification pathways found in strain NG80-2.
28900693	2	78	theme	hemicellulolytic	368:383	arg1	It					302:303	It	302:303	It	302:303	It was selected from over 500 isolates as a genetically tractable hemicellulolytic lactic acid producer, requiring little nutrients.
28900693	2	78	theme	hemicellulolytic	368:383	arg1	producer					397:404	a genetically tractable hemicellulolytic lactic acid producer	344:404	a genetically tractable hemicellulolytic lactic acid producer	344:404	It was selected from over 500 isolates as a genetically tractable hemicellulolytic lactic acid producer, requiring little nutrients.
28900693	4	79	theme	T12	622:624	arg1	genome					605:610	The genome	601:610	The genome of strain T12	601:624	The genome of strain T12 consists of a 3.64 Mb chromosome and two plasmids of 59 and 56 kb.
28024543	1	0	from	features	164:171	arg1	PSG-1					225:229	PSG-1	225:229	PSG-1	225:229	The fine structure in terms of backbone and branch chain features of a bioactive polysaccharide from Ganoderma atrum (PSG-1) was re-elucidated systematically using high performance anion-exchange chromatography (HPAEC), methylation and GLC-MS analysis, and 1D & 2D NMR spectroscopy.
28024543	1	0	from	features	164:171	arg1	atrum					218:222	Ganoderma atrum	208:222	Ganoderma atrum (PSG-1)	208:230	The fine structure in terms of backbone and branch chain features of a bioactive polysaccharide from Ganoderma atrum (PSG-1) was re-elucidated systematically using high performance anion-exchange chromatography (HPAEC), methylation and GLC-MS analysis, and 1D & 2D NMR spectroscopy.
28024543	1	1	theme	GLC-MS	343:348	arg1	analysis					350:357	methylation and GLC-MS analysis	327:357	methylation and GLC-MS analysis	327:357	The fine structure in terms of backbone and branch chain features of a bioactive polysaccharide from Ganoderma atrum (PSG-1) was re-elucidated systematically using high performance anion-exchange chromatography (HPAEC), methylation and GLC-MS analysis, and 1D & 2D NMR spectroscopy.
28024543	4	2	theme	galactose	753:761	arg1	mannose					741:747	mannose	741:747	mannose	741:747	The small amount of mannose and galactose residues were considered to be from the other fraction in PSG which was very difficult to be separated from PSG-1-F0.2.
28024543	4	2	theme	galactose	753:761	arg1	amount					731:736	The small amount	721:736	The small amount of mannose and galactose residues	721:770	The small amount of mannose and galactose residues were considered to be from the other fraction in PSG which was very difficult to be separated from PSG-1-F0.2.
28024543	4	2	theme	galactose	753:761	arg1	galactose					753:761	galactose	753:761	galactose	753:761	The small amount of mannose and galactose residues were considered to be from the other fraction in PSG which was very difficult to be separated from PSG-1-F0.2.
28024543	1	3	theme	branch	151:156	arg1	features					164:171	branch chain features	151:171	branch chain features of a bioactive polysaccharide from Ganoderma atrum (PSG-1)	151:230	The fine structure in terms of backbone and branch chain features of a bioactive polysaccharide from Ganoderma atrum (PSG-1) was re-elucidated systematically using high performance anion-exchange chromatography (HPAEC), methylation and GLC-MS analysis, and 1D & 2D NMR spectroscopy.
28024543	0	4	theme	structure	96:104	arg1	Re-elucidation					69:82	Re-elucidation	69:82	Characterization of a bioactive polysaccharide from Ganoderma atrum: Re-elucidation of the fine structure.	0:105	Characterization of a bioactive polysaccharide from Ganoderma atrum: Re-elucidation of the fine structure.
28024543	0	5	from	Characterization	0:15	arg1	atrum					62:66	Ganoderma atrum	52:66	Ganoderma atrum	52:66	Characterization of a bioactive polysaccharide from Ganoderma atrum: Re-elucidation of the fine structure.
28024543	3	6	theme	-glucan	712:718	arg1	position					693:700	the O-6 position	685:700	the O-6 position of β-(1→3)-glucan	685:718	Based on methylation, multistep partial acid hydrolysis and NMR study, were proposed to substitute at the O-6 position of β-(1→3)-glucan.
28024543	0	7	theme	fine	91:94	arg1	structure					96:104	the fine structure	87:104	the fine structure	87:104	Characterization of a bioactive polysaccharide from Ganoderma atrum: Re-elucidation of the fine structure.
28024543	0	8	from	atrum	62:66	arg1	Characterization					0:15	Characterization	0:15	Characterization of a bioactive polysaccharide from Ganoderma atrum: Re-elucidation of the fine structure.	0:105	Characterization of a bioactive polysaccharide from Ganoderma atrum: Re-elucidation of the fine structure.
28024543	0	8	from	atrum	62:66	arg1	polysaccharide					32:45	a bioactive polysaccharide	20:45	a bioactive polysaccharide from Ganoderma atrum	20:66	Characterization of a bioactive polysaccharide from Ganoderma atrum: Re-elucidation of the fine structure.
28024543	1	9	from	backbone	138:145	arg1	PSG-1					225:229	PSG-1	225:229	PSG-1	225:229	The fine structure in terms of backbone and branch chain features of a bioactive polysaccharide from Ganoderma atrum (PSG-1) was re-elucidated systematically using high performance anion-exchange chromatography (HPAEC), methylation and GLC-MS analysis, and 1D & 2D NMR spectroscopy.
28024543	1	9	from	backbone	138:145	arg1	atrum					218:222	Ganoderma atrum	208:222	Ganoderma atrum (PSG-1)	208:230	The fine structure in terms of backbone and branch chain features of a bioactive polysaccharide from Ganoderma atrum (PSG-1) was re-elucidated systematically using high performance anion-exchange chromatography (HPAEC), methylation and GLC-MS analysis, and 1D & 2D NMR spectroscopy.
28024543	1	10	theme	chain	158:162	arg1	features					164:171	branch chain features	151:171	branch chain features of a bioactive polysaccharide from Ganoderma atrum (PSG-1)	151:230	The fine structure in terms of backbone and branch chain features of a bioactive polysaccharide from Ganoderma atrum (PSG-1) was re-elucidated systematically using high performance anion-exchange chromatography (HPAEC), methylation and GLC-MS analysis, and 1D & 2D NMR spectroscopy.
28024543	2	11	theme	glucuronic	500:509	arg1	acid					511:514	glucuronic acid	500:514	glucuronic acid (15.3%)	500:522	Monosaccharide composition analysis revealed that PSG-1-F0.2 fraction mainly consisted of glucose (73.8%) and glucuronic acid (15.3%), with small amount of mannose (5.7%) and galactose (5.2%).
28024543	2	11	theme	glucuronic	500:509	arg1	%					521:521	15.3%	517:521	15.3%	517:521	Monosaccharide composition analysis revealed that PSG-1-F0.2 fraction mainly consisted of glucose (73.8%) and glucuronic acid (15.3%), with small amount of mannose (5.7%) and galactose (5.2%).
28024543	1	12	theme	1D	364:365	arg1	spectroscopy					376:387	1D & 2D NMR spectroscopy	364:387	1D & 2D NMR spectroscopy	364:387	The fine structure in terms of backbone and branch chain features of a bioactive polysaccharide from Ganoderma atrum (PSG-1) was re-elucidated systematically using high performance anion-exchange chromatography (HPAEC), methylation and GLC-MS analysis, and 1D & 2D NMR spectroscopy.
28024543	4	13	dep	mannose	741:747	arg1	residues					763:770	residues	763:770	residues	763:770	The small amount of mannose and galactose residues were considered to be from the other fraction in PSG which was very difficult to be separated from PSG-1-F0.2.
28024543	1	14	theme	features	164:171	arg1	terms					129:133	terms	129:133	terms of backbone and branch chain features of a bioactive polysaccharide from Ganoderma atrum (PSG-1)	129:230	The fine structure in terms of backbone and branch chain features of a bioactive polysaccharide from Ganoderma atrum (PSG-1) was re-elucidated systematically using high performance anion-exchange chromatography (HPAEC), methylation and GLC-MS analysis, and 1D & 2D NMR spectroscopy.
28024543	1	15	theme	&	367:367	arg1	spectroscopy					376:387	1D & 2D NMR spectroscopy	364:387	1D & 2D NMR spectroscopy	364:387	The fine structure in terms of backbone and branch chain features of a bioactive polysaccharide from Ganoderma atrum (PSG-1) was re-elucidated systematically using high performance anion-exchange chromatography (HPAEC), methylation and GLC-MS analysis, and 1D & 2D NMR spectroscopy.
28024543	2	16	theme	galactose	565:573	arg1	%					558:558	5.7%	555:558	5.7%	555:558	Monosaccharide composition analysis revealed that PSG-1-F0.2 fraction mainly consisted of glucose (73.8%) and glucuronic acid (15.3%), with small amount of mannose (5.7%) and galactose (5.2%).
28024543	2	16	theme	galactose	565:573	arg1	amount					536:541	small amount	530:541	small amount of mannose (5.7%) and galactose (5.2%)	530:580	Monosaccharide composition analysis revealed that PSG-1-F0.2 fraction mainly consisted of glucose (73.8%) and glucuronic acid (15.3%), with small amount of mannose (5.7%) and galactose (5.2%).
28024543	2	16	theme	galactose	565:573	arg1	%					579:579	5.2%	576:579	5.2%	576:579	Monosaccharide composition analysis revealed that PSG-1-F0.2 fraction mainly consisted of glucose (73.8%) and glucuronic acid (15.3%), with small amount of mannose (5.7%) and galactose (5.2%).
28024543	2	16	theme	galactose	565:573	arg1	mannose					546:552	mannose	546:552	mannose (5.7%)	546:559	Monosaccharide composition analysis revealed that PSG-1-F0.2 fraction mainly consisted of glucose (73.8%) and glucuronic acid (15.3%), with small amount of mannose (5.7%) and galactose (5.2%).
28024543	2	16	theme	galactose	565:573	arg1	galactose					565:573	galactose	565:573	galactose (5.2%)	565:580	Monosaccharide composition analysis revealed that PSG-1-F0.2 fraction mainly consisted of glucose (73.8%) and glucuronic acid (15.3%), with small amount of mannose (5.7%) and galactose (5.2%).
28024543	3	17	theme	acid	623:626	arg1	hydrolysis					628:637	multistep partial acid hydrolysis	605:637	multistep partial acid hydrolysis	605:637	Based on methylation, multistep partial acid hydrolysis and NMR study, were proposed to substitute at the O-6 position of β-(1→3)-glucan.
28024543	3	18	theme	partial	615:621	arg1	hydrolysis					628:637	multistep partial acid hydrolysis	605:637	multistep partial acid hydrolysis	605:637	Based on methylation, multistep partial acid hydrolysis and NMR study, were proposed to substitute at the O-6 position of β-(1→3)-glucan.
28024543	1	19	theme	high	271:274	arg1	HPAEC					319:323	HPAEC	319:323	HPAEC	319:323	The fine structure in terms of backbone and branch chain features of a bioactive polysaccharide from Ganoderma atrum (PSG-1) was re-elucidated systematically using high performance anion-exchange chromatography (HPAEC), methylation and GLC-MS analysis, and 1D & 2D NMR spectroscopy.
28024543	1	19	theme	high	271:274	arg1	chromatography					303:316	high performance anion-exchange chromatography	271:316	high performance anion-exchange chromatography (HPAEC)	271:324	The fine structure in terms of backbone and branch chain features of a bioactive polysaccharide from Ganoderma atrum (PSG-1) was re-elucidated systematically using high performance anion-exchange chromatography (HPAEC), methylation and GLC-MS analysis, and 1D & 2D NMR spectroscopy.
28024543	4	20	theme	mannose	741:747	arg1	mannose					741:747	mannose	741:747	mannose	741:747	The small amount of mannose and galactose residues were considered to be from the other fraction in PSG which was very difficult to be separated from PSG-1-F0.2.
28024543	4	20	theme	mannose	741:747	arg1	amount					731:736	The small amount	721:736	The small amount of mannose and galactose residues	721:770	The small amount of mannose and galactose residues were considered to be from the other fraction in PSG which was very difficult to be separated from PSG-1-F0.2.
28024543	4	20	theme	mannose	741:747	arg1	galactose					753:761	galactose	753:761	galactose	753:761	The small amount of mannose and galactose residues were considered to be from the other fraction in PSG which was very difficult to be separated from PSG-1-F0.2.
28024543	0	21	theme	bioactive	22:30	arg1	polysaccharide					32:45	a bioactive polysaccharide	20:45	a bioactive polysaccharide from Ganoderma atrum	20:66	Characterization of a bioactive polysaccharide from Ganoderma atrum: Re-elucidation of the fine structure.
28024543	1	22	theme	performance	276:286	arg1	HPAEC					319:323	HPAEC	319:323	HPAEC	319:323	The fine structure in terms of backbone and branch chain features of a bioactive polysaccharide from Ganoderma atrum (PSG-1) was re-elucidated systematically using high performance anion-exchange chromatography (HPAEC), methylation and GLC-MS analysis, and 1D & 2D NMR spectroscopy.
28024543	1	22	theme	performance	276:286	arg1	chromatography					303:316	high performance anion-exchange chromatography	271:316	high performance anion-exchange chromatography (HPAEC)	271:324	The fine structure in terms of backbone and branch chain features of a bioactive polysaccharide from Ganoderma atrum (PSG-1) was re-elucidated systematically using high performance anion-exchange chromatography (HPAEC), methylation and GLC-MS analysis, and 1D & 2D NMR spectroscopy.
28024543	1	23	from	structure	116:124	arg1	terms					129:133	terms	129:133	terms of backbone and branch chain features of a bioactive polysaccharide from Ganoderma atrum (PSG-1)	129:230	The fine structure in terms of backbone and branch chain features of a bioactive polysaccharide from Ganoderma atrum (PSG-1) was re-elucidated systematically using high performance anion-exchange chromatography (HPAEC), methylation and GLC-MS analysis, and 1D & 2D NMR spectroscopy.
28024543	1	24	theme	bioactive	178:186	arg1	polysaccharide					188:201	a bioactive polysaccharide	176:201	a bioactive polysaccharide from Ganoderma atrum (PSG-1)	176:230	The fine structure in terms of backbone and branch chain features of a bioactive polysaccharide from Ganoderma atrum (PSG-1) was re-elucidated systematically using high performance anion-exchange chromatography (HPAEC), methylation and GLC-MS analysis, and 1D & 2D NMR spectroscopy.
28024543	1	25	theme	anion-exchange	288:301	arg1	HPAEC					319:323	HPAEC	319:323	HPAEC	319:323	The fine structure in terms of backbone and branch chain features of a bioactive polysaccharide from Ganoderma atrum (PSG-1) was re-elucidated systematically using high performance anion-exchange chromatography (HPAEC), methylation and GLC-MS analysis, and 1D & 2D NMR spectroscopy.
28024543	1	25	theme	anion-exchange	288:301	arg1	chromatography					303:316	high performance anion-exchange chromatography	271:316	high performance anion-exchange chromatography (HPAEC)	271:324	The fine structure in terms of backbone and branch chain features of a bioactive polysaccharide from Ganoderma atrum (PSG-1) was re-elucidated systematically using high performance anion-exchange chromatography (HPAEC), methylation and GLC-MS analysis, and 1D & 2D NMR spectroscopy.
28024543	4	26	theme	other	803:807	arg1	fraction					809:816	the other fraction	799:816	the other fraction	799:816	The small amount of mannose and galactose residues were considered to be from the other fraction in PSG which was very difficult to be separated from PSG-1-F0.2.
28024543	1	27	theme	2D	369:370	arg1	spectroscopy					376:387	1D & 2D NMR spectroscopy	364:387	1D & 2D NMR spectroscopy	364:387	The fine structure in terms of backbone and branch chain features of a bioactive polysaccharide from Ganoderma atrum (PSG-1) was re-elucidated systematically using high performance anion-exchange chromatography (HPAEC), methylation and GLC-MS analysis, and 1D & 2D NMR spectroscopy.
28024543	1	28	theme	polysaccharide	188:201	arg1	backbone					138:145	backbone	138:145	backbone	138:145	The fine structure in terms of backbone and branch chain features of a bioactive polysaccharide from Ganoderma atrum (PSG-1) was re-elucidated systematically using high performance anion-exchange chromatography (HPAEC), methylation and GLC-MS analysis, and 1D & 2D NMR spectroscopy.
28024543	1	28	theme	polysaccharide	188:201	arg1	features					164:171	branch chain features	151:171	branch chain features of a bioactive polysaccharide from Ganoderma atrum (PSG-1)	151:230	The fine structure in terms of backbone and branch chain features of a bioactive polysaccharide from Ganoderma atrum (PSG-1) was re-elucidated systematically using high performance anion-exchange chromatography (HPAEC), methylation and GLC-MS analysis, and 1D & 2D NMR spectroscopy.
28024543	5	29	theme	revised	888:894	arg1	structure					896:904	This revised structure	883:904	This revised structure as an acidic β-(1→3, 1→6)-glucan	883:937	This revised structure as an acidic β-(1→3, 1→6)-glucan is considered to be more accurate than the previous proposal of PSG-1.
28024543	1	30	theme	NMR	372:374	arg1	spectroscopy					376:387	1D & 2D NMR spectroscopy	364:387	1D & 2D NMR spectroscopy	364:387	The fine structure in terms of backbone and branch chain features of a bioactive polysaccharide from Ganoderma atrum (PSG-1) was re-elucidated systematically using high performance anion-exchange chromatography (HPAEC), methylation and GLC-MS analysis, and 1D & 2D NMR spectroscopy.
28024543	2	31	theme	composition	405:415	arg1	analysis					417:424	Monosaccharide composition analysis	390:424	Monosaccharide composition analysis	390:424	Monosaccharide composition analysis revealed that PSG-1-F0.2 fraction mainly consisted of glucose (73.8%) and glucuronic acid (15.3%), with small amount of mannose (5.7%) and galactose (5.2%).
28024543	0	32	theme	polysaccharide	32:45	arg1	Characterization					0:15	Characterization	0:15	Characterization of a bioactive polysaccharide from Ganoderma atrum: Re-elucidation of the fine structure.	0:105	Characterization of a bioactive polysaccharide from Ganoderma atrum: Re-elucidation of the fine structure.
28024543	4	33	from	fraction	809:816	arg1	PSG					821:823	PSG	821:823	PSG which was very difficult to be separated from PSG-1-F0.2	821:880	The small amount of mannose and galactose residues were considered to be from the other fraction in PSG which was very difficult to be separated from PSG-1-F0.2.
28024543	1	34	theme	Ganoderma	208:216	arg1	PSG-1					225:229	PSG-1	225:229	PSG-1	225:229	The fine structure in terms of backbone and branch chain features of a bioactive polysaccharide from Ganoderma atrum (PSG-1) was re-elucidated systematically using high performance anion-exchange chromatography (HPAEC), methylation and GLC-MS analysis, and 1D & 2D NMR spectroscopy.
28024543	1	34	theme	Ganoderma	208:216	arg1	atrum					218:222	Ganoderma atrum	208:222	Ganoderma atrum (PSG-1)	208:230	The fine structure in terms of backbone and branch chain features of a bioactive polysaccharide from Ganoderma atrum (PSG-1) was re-elucidated systematically using high performance anion-exchange chromatography (HPAEC), methylation and GLC-MS analysis, and 1D & 2D NMR spectroscopy.
28024543	2	35	theme	mannose	546:552	arg1	%					558:558	5.7%	555:558	5.7%	555:558	Monosaccharide composition analysis revealed that PSG-1-F0.2 fraction mainly consisted of glucose (73.8%) and glucuronic acid (15.3%), with small amount of mannose (5.7%) and galactose (5.2%).
28024543	2	35	theme	mannose	546:552	arg1	amount					536:541	small amount	530:541	small amount of mannose (5.7%) and galactose (5.2%)	530:580	Monosaccharide composition analysis revealed that PSG-1-F0.2 fraction mainly consisted of glucose (73.8%) and glucuronic acid (15.3%), with small amount of mannose (5.7%) and galactose (5.2%).
28024543	2	35	theme	mannose	546:552	arg1	%					579:579	5.2%	576:579	5.2%	576:579	Monosaccharide composition analysis revealed that PSG-1-F0.2 fraction mainly consisted of glucose (73.8%) and glucuronic acid (15.3%), with small amount of mannose (5.7%) and galactose (5.2%).
28024543	2	35	theme	mannose	546:552	arg1	mannose					546:552	mannose	546:552	mannose (5.7%)	546:559	Monosaccharide composition analysis revealed that PSG-1-F0.2 fraction mainly consisted of glucose (73.8%) and glucuronic acid (15.3%), with small amount of mannose (5.7%) and galactose (5.2%).
28024543	2	35	theme	mannose	546:552	arg1	galactose					565:573	galactose	565:573	galactose (5.2%)	565:580	Monosaccharide composition analysis revealed that PSG-1-F0.2 fraction mainly consisted of glucose (73.8%) and glucuronic acid (15.3%), with small amount of mannose (5.7%) and galactose (5.2%).
28024543	4	36	theme	small	725:729	arg1	mannose					741:747	mannose	741:747	mannose	741:747	The small amount of mannose and galactose residues were considered to be from the other fraction in PSG which was very difficult to be separated from PSG-1-F0.2.
28024543	4	36	theme	small	725:729	arg1	amount					731:736	The small amount	721:736	The small amount of mannose and galactose residues	721:770	The small amount of mannose and galactose residues were considered to be from the other fraction in PSG which was very difficult to be separated from PSG-1-F0.2.
28024543	4	36	theme	small	725:729	arg1	galactose					753:761	galactose	753:761	galactose	753:761	The small amount of mannose and galactose residues were considered to be from the other fraction in PSG which was very difficult to be separated from PSG-1-F0.2.
28024543	3	37	theme	NMR	643:645	arg1	study					647:651	NMR study	643:651	NMR study	643:651	Based on methylation, multistep partial acid hydrolysis and NMR study, were proposed to substitute at the O-6 position of β-(1→3)-glucan.
28024543	1	38	theme	fine	111:114	arg1	structure					116:124	The fine structure	107:124	The fine structure in terms of backbone and branch chain features of a bioactive polysaccharide from Ganoderma atrum (PSG-1)	107:230	The fine structure in terms of backbone and branch chain features of a bioactive polysaccharide from Ganoderma atrum (PSG-1) was re-elucidated systematically using high performance anion-exchange chromatography (HPAEC), methylation and GLC-MS analysis, and 1D & 2D NMR spectroscopy.
28024543	0	39	theme	Ganoderma	52:60	arg1	atrum					62:66	Ganoderma atrum	52:66	Ganoderma atrum	52:66	Characterization of a bioactive polysaccharide from Ganoderma atrum: Re-elucidation of the fine structure.
28024543	5	40	theme	PSG-1	1003:1007	arg1	proposal					991:998	the previous proposal	978:998	the previous proposal of PSG-1	978:1007	This revised structure as an acidic β-(1→3, 1→6)-glucan is considered to be more accurate than the previous proposal of PSG-1.
28024543	5	41	theme	β-	919:920	arg1	-glucan					931:937	an acidic β-(1→3, 1→6)-glucan	909:937	an acidic β-(1→3, 1→6)-glucan	909:937	This revised structure as an acidic β-(1→3, 1→6)-glucan is considered to be more accurate than the previous proposal of PSG-1.
28024543	5	42	theme	acidic	912:917	arg1	β-					919:920	an acidic β-	909:920	an acidic β-(1→3, 1→6)-glucan	909:937	This revised structure as an acidic β-(1→3, 1→6)-glucan is considered to be more accurate than the previous proposal of PSG-1.
28024543	3	43	theme	O-6	689:691	arg1	position					693:700	the O-6 position	685:700	the O-6 position of β-(1→3)-glucan	685:718	Based on methylation, multistep partial acid hydrolysis and NMR study, were proposed to substitute at the O-6 position of β-(1→3)-glucan.
28024543	1	44	from	atrum	218:222	arg1	backbone					138:145	backbone	138:145	backbone	138:145	The fine structure in terms of backbone and branch chain features of a bioactive polysaccharide from Ganoderma atrum (PSG-1) was re-elucidated systematically using high performance anion-exchange chromatography (HPAEC), methylation and GLC-MS analysis, and 1D & 2D NMR spectroscopy.
28024543	1	44	from	atrum	218:222	arg1	features					164:171	branch chain features	151:171	branch chain features of a bioactive polysaccharide from Ganoderma atrum (PSG-1)	151:230	The fine structure in terms of backbone and branch chain features of a bioactive polysaccharide from Ganoderma atrum (PSG-1) was re-elucidated systematically using high performance anion-exchange chromatography (HPAEC), methylation and GLC-MS analysis, and 1D & 2D NMR spectroscopy.
28024543	1	44	from	atrum	218:222	arg1	polysaccharide					188:201	a bioactive polysaccharide	176:201	a bioactive polysaccharide from Ganoderma atrum (PSG-1)	176:230	The fine structure in terms of backbone and branch chain features of a bioactive polysaccharide from Ganoderma atrum (PSG-1) was re-elucidated systematically using high performance anion-exchange chromatography (HPAEC), methylation and GLC-MS analysis, and 1D & 2D NMR spectroscopy.
28024543	3	45	theme	multistep	605:613	arg1	hydrolysis					628:637	multistep partial acid hydrolysis	605:637	multistep partial acid hydrolysis	605:637	Based on methylation, multistep partial acid hydrolysis and NMR study, were proposed to substitute at the O-6 position of β-(1→3)-glucan.
28024543	3	46	theme	1→3	708:710	arg1	-glucan					712:718	β-(1→3)-glucan	705:718	β-(1→3)-glucan	705:718	Based on methylation, multistep partial acid hydrolysis and NMR study, were proposed to substitute at the O-6 position of β-(1→3)-glucan.
28024543	2	47	theme	small	530:534	arg1	%					558:558	5.7%	555:558	5.7%	555:558	Monosaccharide composition analysis revealed that PSG-1-F0.2 fraction mainly consisted of glucose (73.8%) and glucuronic acid (15.3%), with small amount of mannose (5.7%) and galactose (5.2%).
28024543	2	47	theme	small	530:534	arg1	amount					536:541	small amount	530:541	small amount of mannose (5.7%) and galactose (5.2%)	530:580	Monosaccharide composition analysis revealed that PSG-1-F0.2 fraction mainly consisted of glucose (73.8%) and glucuronic acid (15.3%), with small amount of mannose (5.7%) and galactose (5.2%).
28024543	2	47	theme	small	530:534	arg1	%					579:579	5.2%	576:579	5.2%	576:579	Monosaccharide composition analysis revealed that PSG-1-F0.2 fraction mainly consisted of glucose (73.8%) and glucuronic acid (15.3%), with small amount of mannose (5.7%) and galactose (5.2%).
28024543	2	47	theme	small	530:534	arg1	mannose					546:552	mannose	546:552	mannose (5.7%)	546:559	Monosaccharide composition analysis revealed that PSG-1-F0.2 fraction mainly consisted of glucose (73.8%) and glucuronic acid (15.3%), with small amount of mannose (5.7%) and galactose (5.2%).
28024543	2	47	theme	small	530:534	arg1	galactose					565:573	galactose	565:573	galactose (5.2%)	565:580	Monosaccharide composition analysis revealed that PSG-1-F0.2 fraction mainly consisted of glucose (73.8%) and glucuronic acid (15.3%), with small amount of mannose (5.7%) and galactose (5.2%).
28024543	2	48	theme	Monosaccharide	390:403	arg1	analysis					417:424	Monosaccharide composition analysis	390:424	Monosaccharide composition analysis	390:424	Monosaccharide composition analysis revealed that PSG-1-F0.2 fraction mainly consisted of glucose (73.8%) and glucuronic acid (15.3%), with small amount of mannose (5.7%) and galactose (5.2%).
28024543	5	49	theme	previous	982:989	arg1	proposal					991:998	the previous proposal	978:998	the previous proposal of PSG-1	978:1007	This revised structure as an acidic β-(1→3, 1→6)-glucan is considered to be more accurate than the previous proposal of PSG-1.
28024543	3	50	theme	β-	705:706	arg1	-glucan					712:718	β-(1→3)-glucan	705:718	β-(1→3)-glucan	705:718	Based on methylation, multistep partial acid hydrolysis and NMR study, were proposed to substitute at the O-6 position of β-(1→3)-glucan.
28024543	5	51	dep	β-	919:920	arg1	1→3					922:924	1→3	922:924	1→3	922:924	This revised structure as an acidic β-(1→3, 1→6)-glucan is considered to be more accurate than the previous proposal of PSG-1.
28024543	5	51	dep	β-	919:920	arg1	1→6					927:929	1→6	927:929	1→6	927:929	This revised structure as an acidic β-(1→3, 1→6)-glucan is considered to be more accurate than the previous proposal of PSG-1.
28024543	1	52	theme	methylation	327:337	arg1	analysis					350:357	methylation and GLC-MS analysis	327:357	methylation and GLC-MS analysis	327:357	The fine structure in terms of backbone and branch chain features of a bioactive polysaccharide from Ganoderma atrum (PSG-1) was re-elucidated systematically using high performance anion-exchange chromatography (HPAEC), methylation and GLC-MS analysis, and 1D & 2D NMR spectroscopy.
28024543	0	53	dep	Characterization	0:15	arg1	Re-elucidation					69:82	Re-elucidation	69:82	Characterization of a bioactive polysaccharide from Ganoderma atrum: Re-elucidation of the fine structure.	0:105	Characterization of a bioactive polysaccharide from Ganoderma atrum: Re-elucidation of the fine structure.
28024543	1	54	theme	backbone	138:145	arg1	terms					129:133	terms	129:133	terms of backbone and branch chain features of a bioactive polysaccharide from Ganoderma atrum (PSG-1)	129:230	The fine structure in terms of backbone and branch chain features of a bioactive polysaccharide from Ganoderma atrum (PSG-1) was re-elucidated systematically using high performance anion-exchange chromatography (HPAEC), methylation and GLC-MS analysis, and 1D & 2D NMR spectroscopy.
28235606	0	0	theme	lung	85:88	arg1	cancer					90:95	human non-small cell lung cancer	64:95	human non-small cell lung cancer	64:95	Anti-tumor effect of polysaccharide from Hirsutella sinensis on human non-small cell lung cancer and nude mice through intrinsic mitochondrial pathway.
28235606	6	1	dep	expressions	1013:1023	arg1	decreased					1084:1092	decreased	1084:1092	were also decreased	1074:1092	Blue native Polyacrylamide Gel-Electrophoresis (PAGE) showed the expressions of mitochondrial respiratory chain complexes I-V were also decreased.
28235606	7	2	theme	apoptosis	1175:1183	arg1	pathway					1195:1201	intrinsic mitochondrial apoptosis mechanism pathway	1151:1201	intrinsic mitochondrial apoptosis mechanism pathway and involving ROS increasing	1151:1230	Taken together, anti-tumor effect of HSP-III is through intrinsic mitochondrial apoptosis mechanism pathway and involving ROS increasing.
28235606	5	3	theme	apoptotic	860:868	arg1	effects					870:876	the apoptotic effects	856:876	the apoptotic effects of HSP-III	856:887	Furthermore, we found the apoptotic effects of HSP-III are triggered by Reactive oxygen species (ROS) generation.
28235606	1	4	theme	previous	156:163	arg1	works					165:169	Our previous works	152:169	Our previous works	152:169	Our previous works had proved the structural properties of Hirsutella sinensis polysaccharide-III(HSP-III).
28235606	10	5	theme	H1299	1490:1494	arg1	cells					1496:1500	H1299 cells	1490:1500	H1299 cells	1490:1500	These findings suggest that HSP-III induce apoptosis of H1299 cells and attenuated growth of nude mice tumor in vivo through the intrinsic mitochondrial pathway and stimulating ROS.
28235606	6	6	theme	respiratory	1042:1052	arg1	complexes					1060:1068	mitochondrial respiratory chain complexes	1028:1068	mitochondrial respiratory chain complexes	1028:1068	Blue native Polyacrylamide Gel-Electrophoresis (PAGE) showed the expressions of mitochondrial respiratory chain complexes I-V were also decreased.
28235606	0	7	theme	cell	80:83	arg1	cancer					90:95	human non-small cell lung cancer	64:95	human non-small cell lung cancer	64:95	Anti-tumor effect of polysaccharide from Hirsutella sinensis on human non-small cell lung cancer and nude mice through intrinsic mitochondrial pathway.
28235606	0	8	from	effect	11:16	arg1	sinensis					52:59	Hirsutella sinensis	41:59	Hirsutella sinensis	41:59	Anti-tumor effect of polysaccharide from Hirsutella sinensis on human non-small cell lung cancer and nude mice through intrinsic mitochondrial pathway.
28235606	0	8	from	effect	11:16	arg1	cancer					90:95	human non-small cell lung cancer	64:95	human non-small cell lung cancer	64:95	Anti-tumor effect of polysaccharide from Hirsutella sinensis on human non-small cell lung cancer and nude mice through intrinsic mitochondrial pathway.
28235606	0	8	from	effect	11:16	arg1	mice					106:109	nude mice	101:109	nude mice	101:109	Anti-tumor effect of polysaccharide from Hirsutella sinensis on human non-small cell lung cancer and nude mice through intrinsic mitochondrial pathway.
28235606	7	9	theme	mechanism	1185:1193	arg1	pathway					1195:1201	intrinsic mitochondrial apoptosis mechanism pathway	1151:1201	intrinsic mitochondrial apoptosis mechanism pathway and involving ROS increasing	1151:1230	Taken together, anti-tumor effect of HSP-III is through intrinsic mitochondrial apoptosis mechanism pathway and involving ROS increasing.
28235606	4	10	theme	caspase-3	712:720	arg1	analysis					681:688	western blot analysis	668:688	western blot analysis of cytochrome C, Bax, caspase-3 and caspase-9	668:734	HSP-III treatment collapses the H1299 cell mitochondrial membrane potential, and western blot analysis of cytochrome C, Bax, caspase-3 and caspase-9 further indicates that apoptotic effects induced by HSP-III is through the mitochondrial pathway.
28235606	5	11	theme	species	922:928	arg1	generation					936:945	Reactive oxygen species (ROS) generation	906:945	Reactive oxygen species (ROS) generation	906:945	Furthermore, we found the apoptotic effects of HSP-III are triggered by Reactive oxygen species (ROS) generation.
28235606	7	12	theme	intrinsic	1151:1159	arg1	pathway					1195:1201	intrinsic mitochondrial apoptosis mechanism pathway	1151:1201	intrinsic mitochondrial apoptosis mechanism pathway and involving ROS increasing	1151:1230	Taken together, anti-tumor effect of HSP-III is through intrinsic mitochondrial apoptosis mechanism pathway and involving ROS increasing.
28235606	2	13	theme	lung	293:296	arg1	cancer					298:303	lung cancer	293:303	lung cancer	293:303	Herein, its anti-tumor effect on lung cancer correlated with mitochondrial apoptosis pathway was investigated.
28235606	4	14	theme	cell	625:628	arg1	potential					653:661	the H1299 cell mitochondrial membrane potential	615:661	the H1299 cell mitochondrial membrane potential	615:661	HSP-III treatment collapses the H1299 cell mitochondrial membrane potential, and western blot analysis of cytochrome C, Bax, caspase-3 and caspase-9 further indicates that apoptotic effects induced by HSP-III is through the mitochondrial pathway.
28235606	7	15	theme	mitochondrial	1161:1173	arg1	pathway					1195:1201	intrinsic mitochondrial apoptosis mechanism pathway	1151:1201	intrinsic mitochondrial apoptosis mechanism pathway and involving ROS increasing	1151:1230	Taken together, anti-tumor effect of HSP-III is through intrinsic mitochondrial apoptosis mechanism pathway and involving ROS increasing.
28235606	4	16	theme	H1299	619:623	arg1	potential					653:661	the H1299 cell mitochondrial membrane potential	615:661	the H1299 cell mitochondrial membrane potential	615:661	HSP-III treatment collapses the H1299 cell mitochondrial membrane potential, and western blot analysis of cytochrome C, Bax, caspase-3 and caspase-9 further indicates that apoptotic effects induced by HSP-III is through the mitochondrial pathway.
28235606	0	17	from	sinensis	52:59	arg1	polysaccharide					21:34	polysaccharide	21:34	polysaccharide from Hirsutella sinensis	21:59	Anti-tumor effect of polysaccharide from Hirsutella sinensis on human non-small cell lung cancer and nude mice through intrinsic mitochondrial pathway.
28235606	0	17	from	sinensis	52:59	arg1	effect					11:16	Anti-tumor effect	0:16	Anti-tumor effect of polysaccharide from Hirsutella sinensis on human non-small cell lung cancer and nude mice through intrinsic mitochondrial pathway.	0:150	Anti-tumor effect of polysaccharide from Hirsutella sinensis on human non-small cell lung cancer and nude mice through intrinsic mitochondrial pathway.
28235606	7	18	dep	pathway	1195:1201	arg1	involving					1207:1215	involving	1207:1215	involving ROS increasing	1207:1230	Taken together, anti-tumor effect of HSP-III is through intrinsic mitochondrial apoptosis mechanism pathway and involving ROS increasing.
28235606	8	19	dep	in	1242:1243	arg1	vivo					1245:1248	vivo	1245:1248	vivo	1245:1248	Finally, in vivo nude mice experiment, HSP-III attenuated the growth of tumor compared with control.
28235606	3	20	theme	epithelial	553:562	arg1	cells					564:568	normal lung epithelial cells	541:568	normal lung epithelial cells	541:568	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay showed that HSP-III induces the apoptosis of H1299 cells; however the proliferation viability of normal lung epithelial cells is not affected.
28235606	8	21	theme	nude	1250:1253	arg1	mice					1255:1258	in vivo nude mice experiment, HSP-III	1242:1278	in vivo nude mice experiment, HSP-III	1242:1278	Finally, in vivo nude mice experiment, HSP-III attenuated the growth of tumor compared with control.
28235606	4	22	theme	cytochrome	693:702	arg1	C					704:704	cytochrome C	693:704	cytochrome C	693:704	HSP-III treatment collapses the H1299 cell mitochondrial membrane potential, and western blot analysis of cytochrome C, Bax, caspase-3 and caspase-9 further indicates that apoptotic effects induced by HSP-III is through the mitochondrial pathway.
28235606	0	23	theme	nude	101:104	arg1	mice					106:109	nude mice	101:109	nude mice	101:109	Anti-tumor effect of polysaccharide from Hirsutella sinensis on human non-small cell lung cancer and nude mice through intrinsic mitochondrial pathway.
28235606	2	24	theme	anti-tumor	272:281	arg1	effect					283:288	its anti-tumor effect	268:288	its anti-tumor effect on lung cancer	268:303	Herein, its anti-tumor effect on lung cancer correlated with mitochondrial apoptosis pathway was investigated.
28235606	3	25	theme	bromide	424:430	arg1	assay					438:442	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay	371:442	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay	371:442	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay showed that HSP-III induces the apoptosis of H1299 cells; however the proliferation viability of normal lung epithelial cells is not affected.
28235606	8	26	theme	in	1242:1243	arg1	mice					1255:1258	in vivo nude mice experiment, HSP-III	1242:1278	in vivo nude mice experiment, HSP-III	1242:1278	Finally, in vivo nude mice experiment, HSP-III attenuated the growth of tumor compared with control.
28235606	2	27	theme	mitochondrial	321:333	arg1	pathway					345:351	mitochondrial apoptosis pathway	321:351	mitochondrial apoptosis pathway	321:351	Herein, its anti-tumor effect on lung cancer correlated with mitochondrial apoptosis pathway was investigated.
28235606	10	28	theme	intrinsic	1563:1571	arg1	pathway					1587:1593	the intrinsic mitochondrial pathway	1559:1593	the intrinsic mitochondrial pathway	1559:1593	These findings suggest that HSP-III induce apoptosis of H1299 cells and attenuated growth of nude mice tumor in vivo through the intrinsic mitochondrial pathway and stimulating ROS.
28235606	8	29	theme	tumor	1305:1309	arg1	growth					1295:1300	the growth	1291:1300	the growth of tumor	1291:1309	Finally, in vivo nude mice experiment, HSP-III attenuated the growth of tumor compared with control.
28235606	0	30	theme	Anti-tumor	0:9	arg1	effect					11:16	Anti-tumor effect	0:16	Anti-tumor effect of polysaccharide from Hirsutella sinensis on human non-small cell lung cancer and nude mice through intrinsic mitochondrial pathway.	0:150	Anti-tumor effect of polysaccharide from Hirsutella sinensis on human non-small cell lung cancer and nude mice through intrinsic mitochondrial pathway.
28235606	7	31	theme	HSP-III	1132:1138	arg1	effect					1122:1127	anti-tumor effect	1111:1127	anti-tumor effect of HSP-III	1111:1138	Taken together, anti-tumor effect of HSP-III is through intrinsic mitochondrial apoptosis mechanism pathway and involving ROS increasing.
28235606	4	32	theme	apoptotic	759:767	arg1	effects					769:775	apoptotic effects	759:775	apoptotic effects induced by HSP-III	759:794	HSP-III treatment collapses the H1299 cell mitochondrial membrane potential, and western blot analysis of cytochrome C, Bax, caspase-3 and caspase-9 further indicates that apoptotic effects induced by HSP-III is through the mitochondrial pathway.
28235606	1	33	theme	structural	186:195	arg1	properties					197:206	the structural properties	182:206	the structural properties of Hirsutella sinensis polysaccharide-III(HSP-III)	182:257	Our previous works had proved the structural properties of Hirsutella sinensis polysaccharide-III(HSP-III).
28235606	0	34	theme	polysaccharide	21:34	arg1	effect					11:16	Anti-tumor effect	0:16	Anti-tumor effect of polysaccharide from Hirsutella sinensis on human non-small cell lung cancer and nude mice through intrinsic mitochondrial pathway.	0:150	Anti-tumor effect of polysaccharide from Hirsutella sinensis on human non-small cell lung cancer and nude mice through intrinsic mitochondrial pathway.
28235606	0	35	theme	intrinsic	119:127	arg1	pathway					143:149	intrinsic mitochondrial pathway	119:149	intrinsic mitochondrial pathway	119:149	Anti-tumor effect of polysaccharide from Hirsutella sinensis on human non-small cell lung cancer and nude mice through intrinsic mitochondrial pathway.
28235606	10	36	theme	attenuated	1506:1515	arg1	growth					1517:1522	attenuated growth	1506:1522	attenuated growth of nude mice tumor	1506:1541	These findings suggest that HSP-III induce apoptosis of H1299 cells and attenuated growth of nude mice tumor in vivo through the intrinsic mitochondrial pathway and stimulating ROS.
28235606	3	37	theme	H1299	489:493	arg1	cells					495:499	H1299 cells	489:499	H1299 cells	489:499	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay showed that HSP-III induces the apoptosis of H1299 cells; however the proliferation viability of normal lung epithelial cells is not affected.
28235606	3	38	theme	-2,5-diphenyltetrazolium	399:422	arg1	assay					438:442	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay	371:442	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay	371:442	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay showed that HSP-III induces the apoptosis of H1299 cells; however the proliferation viability of normal lung epithelial cells is not affected.
28235606	9	39	theme	cell	1391:1394	arg1	effects					1406:1412	the cell apoptosis effects	1387:1412	the cell apoptosis effects induced by HSP-III	1387:1431	In contrast, N-acetyl-l-cysteine (NAC) could restore the cell apoptosis effects induced by HSP-III.
28235606	4	40	theme	membrane	644:651	arg1	potential					653:661	the H1299 cell mitochondrial membrane potential	615:661	the H1299 cell mitochondrial membrane potential	615:661	HSP-III treatment collapses the H1299 cell mitochondrial membrane potential, and western blot analysis of cytochrome C, Bax, caspase-3 and caspase-9 further indicates that apoptotic effects induced by HSP-III is through the mitochondrial pathway.
28235606	3	41	theme	cells	495:499	arg1	apoptosis					476:484	the apoptosis	472:484	the apoptosis of H1299 cells	472:499	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay showed that HSP-III induces the apoptosis of H1299 cells; however the proliferation viability of normal lung epithelial cells is not affected.
28235606	4	42	theme	HSP-III	587:593	arg1	treatment					595:603	HSP-III treatment	587:603	HSP-III treatment	587:603	HSP-III treatment collapses the H1299 cell mitochondrial membrane potential, and western blot analysis of cytochrome C, Bax, caspase-3 and caspase-9 further indicates that apoptotic effects induced by HSP-III is through the mitochondrial pathway.
28235606	10	43	theme	tumor	1537:1541	arg1	growth					1517:1522	attenuated growth	1506:1522	attenuated growth of nude mice tumor	1506:1541	These findings suggest that HSP-III induce apoptosis of H1299 cells and attenuated growth of nude mice tumor in vivo through the intrinsic mitochondrial pathway and stimulating ROS.
28235606	10	43	theme	tumor	1537:1541	arg1	apoptosis					1477:1485	apoptosis	1477:1485	apoptosis of H1299 cells	1477:1500	These findings suggest that HSP-III induce apoptosis of H1299 cells and attenuated growth of nude mice tumor in vivo through the intrinsic mitochondrial pathway and stimulating ROS.
28235606	10	44	theme	mitochondrial	1573:1585	arg1	pathway					1587:1593	the intrinsic mitochondrial pathway	1559:1593	the intrinsic mitochondrial pathway	1559:1593	These findings suggest that HSP-III induce apoptosis of H1299 cells and attenuated growth of nude mice tumor in vivo through the intrinsic mitochondrial pathway and stimulating ROS.
28235606	4	45	theme	C	704:704	arg1	analysis					681:688	western blot analysis	668:688	western blot analysis of cytochrome C, Bax, caspase-3 and caspase-9	668:734	HSP-III treatment collapses the H1299 cell mitochondrial membrane potential, and western blot analysis of cytochrome C, Bax, caspase-3 and caspase-9 further indicates that apoptotic effects induced by HSP-III is through the mitochondrial pathway.
28235606	1	46	theme	Hirsutella	211:220	arg1	HSP-III					250:256	HSP-III	250:256	HSP-III	250:256	Our previous works had proved the structural properties of Hirsutella sinensis polysaccharide-III(HSP-III).
28235606	1	46	theme	Hirsutella	211:220	arg1	polysaccharide-III					231:248	Hirsutella sinensis polysaccharide-III	211:248	Hirsutella sinensis polysaccharide-III(HSP-III)	211:257	Our previous works had proved the structural properties of Hirsutella sinensis polysaccharide-III(HSP-III).
28235606	0	47	theme	mitochondrial	129:141	arg1	pathway					143:149	intrinsic mitochondrial pathway	119:149	intrinsic mitochondrial pathway	119:149	Anti-tumor effect of polysaccharide from Hirsutella sinensis on human non-small cell lung cancer and nude mice through intrinsic mitochondrial pathway.
28235606	6	48	theme	mitochondrial	1028:1040	arg1	complexes					1060:1068	mitochondrial respiratory chain complexes	1028:1068	mitochondrial respiratory chain complexes	1028:1068	Blue native Polyacrylamide Gel-Electrophoresis (PAGE) showed the expressions of mitochondrial respiratory chain complexes I-V were also decreased.
28235606	7	49	theme	increasing	1221:1230	arg1	ROS					1217:1219	ROS increasing	1217:1230	ROS increasing	1217:1230	Taken together, anti-tumor effect of HSP-III is through intrinsic mitochondrial apoptosis mechanism pathway and involving ROS increasing.
28235606	5	50	theme	HSP-III	881:887	arg1	effects					870:876	the apoptotic effects	856:876	the apoptotic effects of HSP-III	856:887	Furthermore, we found the apoptotic effects of HSP-III are triggered by Reactive oxygen species (ROS) generation.
28235606	1	51	theme	sinensis	222:229	arg1	HSP-III					250:256	HSP-III	250:256	HSP-III	250:256	Our previous works had proved the structural properties of Hirsutella sinensis polysaccharide-III(HSP-III).
28235606	1	51	theme	sinensis	222:229	arg1	polysaccharide-III					231:248	Hirsutella sinensis polysaccharide-III	211:248	Hirsutella sinensis polysaccharide-III(HSP-III)	211:257	Our previous works had proved the structural properties of Hirsutella sinensis polysaccharide-III(HSP-III).
28235606	5	52	theme	oxygen	915:920	arg1	ROS					931:933	ROS	931:933	ROS	931:933	Furthermore, we found the apoptotic effects of HSP-III are triggered by Reactive oxygen species (ROS) generation.
28235606	5	52	theme	oxygen	915:920	arg1	species					922:928	Reactive oxygen species	906:928	Reactive oxygen species (ROS) generation	906:945	Furthermore, we found the apoptotic effects of HSP-III are triggered by Reactive oxygen species (ROS) generation.
28235606	8	53	dep	mice	1255:1258	arg1	HSP-III					1272:1278	HSP-III	1272:1278	HSP-III	1272:1278	Finally, in vivo nude mice experiment, HSP-III attenuated the growth of tumor compared with control.
28235606	8	53	dep	mice	1255:1258	arg1	experiment					1260:1269	experiment	1260:1269	experiment	1260:1269	Finally, in vivo nude mice experiment, HSP-III attenuated the growth of tumor compared with control.
28235606	11	54	theme	lung	1651:1654	arg1	treatment					1663:1671	lung cancer treatment	1651:1671	lung cancer treatment	1651:1671	HSP-III could be a composition for lung cancer treatment.
28235606	10	55	theme	cells	1496:1500	arg1	growth					1517:1522	attenuated growth	1506:1522	attenuated growth of nude mice tumor	1506:1541	These findings suggest that HSP-III induce apoptosis of H1299 cells and attenuated growth of nude mice tumor in vivo through the intrinsic mitochondrial pathway and stimulating ROS.
28235606	10	55	theme	cells	1496:1500	arg1	apoptosis					1477:1485	apoptosis	1477:1485	apoptosis of H1299 cells	1477:1500	These findings suggest that HSP-III induce apoptosis of H1299 cells and attenuated growth of nude mice tumor in vivo through the intrinsic mitochondrial pathway and stimulating ROS.
28235606	10	56	theme	mice	1532:1535	arg1	tumor					1537:1541	nude mice tumor	1527:1541	nude mice tumor	1527:1541	These findings suggest that HSP-III induce apoptosis of H1299 cells and attenuated growth of nude mice tumor in vivo through the intrinsic mitochondrial pathway and stimulating ROS.
28235606	6	57	theme	Polyacrylamide	960:973	arg1	Gel-Electrophoresis					975:993	Blue native Polyacrylamide Gel-Electrophoresis	948:993	Blue native Polyacrylamide Gel-Electrophoresis (PAGE)	948:1000	Blue native Polyacrylamide Gel-Electrophoresis (PAGE) showed the expressions of mitochondrial respiratory chain complexes I-V were also decreased.
28235606	6	57	theme	Polyacrylamide	960:973	arg1	PAGE					996:999	PAGE	996:999	PAGE	996:999	Blue native Polyacrylamide Gel-Electrophoresis (PAGE) showed the expressions of mitochondrial respiratory chain complexes I-V were also decreased.
28235606	7	58	theme	anti-tumor	1111:1120	arg1	effect					1122:1127	anti-tumor effect	1111:1127	anti-tumor effect of HSP-III	1111:1138	Taken together, anti-tumor effect of HSP-III is through intrinsic mitochondrial apoptosis mechanism pathway and involving ROS increasing.
28235606	3	59	theme	proliferation	514:526	arg1	viability					528:536	the proliferation viability	510:536	the proliferation viability of normal lung epithelial cells	510:568	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay showed that HSP-III induces the apoptosis of H1299 cells; however the proliferation viability of normal lung epithelial cells is not affected.
28235606	2	60	from	effect	283:288	arg1	cancer					298:303	lung cancer	293:303	lung cancer	293:303	Herein, its anti-tumor effect on lung cancer correlated with mitochondrial apoptosis pathway was investigated.
28235606	6	61	theme	native	953:958	arg1	Gel-Electrophoresis					975:993	Blue native Polyacrylamide Gel-Electrophoresis	948:993	Blue native Polyacrylamide Gel-Electrophoresis (PAGE)	948:1000	Blue native Polyacrylamide Gel-Electrophoresis (PAGE) showed the expressions of mitochondrial respiratory chain complexes I-V were also decreased.
28235606	6	61	theme	native	953:958	arg1	PAGE					996:999	PAGE	996:999	PAGE	996:999	Blue native Polyacrylamide Gel-Electrophoresis (PAGE) showed the expressions of mitochondrial respiratory chain complexes I-V were also decreased.
28235606	9	62	theme	apoptosis	1396:1404	arg1	effects					1406:1412	the cell apoptosis effects	1387:1412	the cell apoptosis effects induced by HSP-III	1387:1431	In contrast, N-acetyl-l-cysteine (NAC) could restore the cell apoptosis effects induced by HSP-III.
28235606	4	63	theme	Bax	707:709	arg1	analysis					681:688	western blot analysis	668:688	western blot analysis of cytochrome C, Bax, caspase-3 and caspase-9	668:734	HSP-III treatment collapses the H1299 cell mitochondrial membrane potential, and western blot analysis of cytochrome C, Bax, caspase-3 and caspase-9 further indicates that apoptotic effects induced by HSP-III is through the mitochondrial pathway.
28235606	5	64	theme	Reactive	906:913	arg1	ROS					931:933	ROS	931:933	ROS	931:933	Furthermore, we found the apoptotic effects of HSP-III are triggered by Reactive oxygen species (ROS) generation.
28235606	5	64	theme	Reactive	906:913	arg1	species					922:928	Reactive oxygen species	906:928	Reactive oxygen species (ROS) generation	906:945	Furthermore, we found the apoptotic effects of HSP-III are triggered by Reactive oxygen species (ROS) generation.
28235606	1	65	theme	polysaccharide-III	231:248	arg1	properties					197:206	the structural properties	182:206	the structural properties of Hirsutella sinensis polysaccharide-III(HSP-III)	182:257	Our previous works had proved the structural properties of Hirsutella sinensis polysaccharide-III(HSP-III).
28235606	3	66	theme	cells	564:568	arg1	viability					528:536	the proliferation viability	510:536	the proliferation viability of normal lung epithelial cells	510:568	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay showed that HSP-III induces the apoptosis of H1299 cells; however the proliferation viability of normal lung epithelial cells is not affected.
28235606	4	67	theme	mitochondrial	811:823	arg1	pathway					825:831	the mitochondrial pathway	807:831	the mitochondrial pathway	807:831	HSP-III treatment collapses the H1299 cell mitochondrial membrane potential, and western blot analysis of cytochrome C, Bax, caspase-3 and caspase-9 further indicates that apoptotic effects induced by HSP-III is through the mitochondrial pathway.
28235606	10	68	theme	nude	1527:1530	arg1	mice					1532:1535	nude mice	1527:1535	nude mice tumor	1527:1541	These findings suggest that HSP-III induce apoptosis of H1299 cells and attenuated growth of nude mice tumor in vivo through the intrinsic mitochondrial pathway and stimulating ROS.
28235606	2	69	theme	apoptosis	335:343	arg1	pathway					345:351	mitochondrial apoptosis pathway	321:351	mitochondrial apoptosis pathway	321:351	Herein, its anti-tumor effect on lung cancer correlated with mitochondrial apoptosis pathway was investigated.
28235606	4	70	theme	mitochondrial	630:642	arg1	potential					653:661	the H1299 cell mitochondrial membrane potential	615:661	the H1299 cell mitochondrial membrane potential	615:661	HSP-III treatment collapses the H1299 cell mitochondrial membrane potential, and western blot analysis of cytochrome C, Bax, caspase-3 and caspase-9 further indicates that apoptotic effects induced by HSP-III is through the mitochondrial pathway.
28235606	0	71	theme	non-small	70:78	arg1	cancer					90:95	human non-small cell lung cancer	64:95	human non-small cell lung cancer	64:95	Anti-tumor effect of polysaccharide from Hirsutella sinensis on human non-small cell lung cancer and nude mice through intrinsic mitochondrial pathway.
28235606	4	72	theme	blot	676:679	arg1	analysis					681:688	western blot analysis	668:688	western blot analysis of cytochrome C, Bax, caspase-3 and caspase-9	668:734	HSP-III treatment collapses the H1299 cell mitochondrial membrane potential, and western blot analysis of cytochrome C, Bax, caspase-3 and caspase-9 further indicates that apoptotic effects induced by HSP-III is through the mitochondrial pathway.
28235606	4	73	theme	western	668:674	arg1	analysis					681:688	western blot analysis	668:688	western blot analysis of cytochrome C, Bax, caspase-3 and caspase-9	668:734	HSP-III treatment collapses the H1299 cell mitochondrial membrane potential, and western blot analysis of cytochrome C, Bax, caspase-3 and caspase-9 further indicates that apoptotic effects induced by HSP-III is through the mitochondrial pathway.
28235606	3	74	theme	normal	541:546	arg1	cells					564:568	normal lung epithelial cells	541:568	normal lung epithelial cells	541:568	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay showed that HSP-III induces the apoptosis of H1299 cells; however the proliferation viability of normal lung epithelial cells is not affected.
28235606	6	75	theme	complexes	1060:1068	arg1	expressions					1013:1023	the expressions	1009:1023	the expressions of mitochondrial respiratory chain complexes I-V were also decreased	1009:1092	Blue native Polyacrylamide Gel-Electrophoresis (PAGE) showed the expressions of mitochondrial respiratory chain complexes I-V were also decreased.
28235606	0	76	theme	human	64:68	arg1	cancer					90:95	human non-small cell lung cancer	64:95	human non-small cell lung cancer	64:95	Anti-tumor effect of polysaccharide from Hirsutella sinensis on human non-small cell lung cancer and nude mice through intrinsic mitochondrial pathway.
28235606	6	77	theme	Blue	948:951	arg1	Gel-Electrophoresis					975:993	Blue native Polyacrylamide Gel-Electrophoresis	948:993	Blue native Polyacrylamide Gel-Electrophoresis (PAGE)	948:1000	Blue native Polyacrylamide Gel-Electrophoresis (PAGE) showed the expressions of mitochondrial respiratory chain complexes I-V were also decreased.
28235606	6	77	theme	Blue	948:951	arg1	PAGE					996:999	PAGE	996:999	PAGE	996:999	Blue native Polyacrylamide Gel-Electrophoresis (PAGE) showed the expressions of mitochondrial respiratory chain complexes I-V were also decreased.
28235606	6	78	theme	chain	1054:1058	arg1	complexes					1060:1068	mitochondrial respiratory chain complexes	1028:1068	mitochondrial respiratory chain complexes	1028:1068	Blue native Polyacrylamide Gel-Electrophoresis (PAGE) showed the expressions of mitochondrial respiratory chain complexes I-V were also decreased.
28235606	11	79	theme	cancer	1656:1661	arg1	treatment					1663:1671	lung cancer treatment	1651:1671	lung cancer treatment	1651:1671	HSP-III could be a composition for lung cancer treatment.
28235606	3	80	theme	lung	548:551	arg1	cells					564:568	normal lung epithelial cells	541:568	normal lung epithelial cells	541:568	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay showed that HSP-III induces the apoptosis of H1299 cells; however the proliferation viability of normal lung epithelial cells is not affected.
28235606	4	81	theme	caspase-9	726:734	arg1	analysis					681:688	western blot analysis	668:688	western blot analysis of cytochrome C, Bax, caspase-3 and caspase-9	668:734	HSP-III treatment collapses the H1299 cell mitochondrial membrane potential, and western blot analysis of cytochrome C, Bax, caspase-3 and caspase-9 further indicates that apoptotic effects induced by HSP-III is through the mitochondrial pathway.
25970893	9	0	theme	aliphatic>	1617:1626	arg1	polysaccharide					1637:1650	aliphatic> protein> polysaccharide	1617:1650	aliphatic> protein> polysaccharide	1617:1650	The result from two-dimensional correlation analysis showed that, organic compounds in humic acids were degraded in the following sequence: aliphatic> protein> polysaccharide and lignin, while that in fulvic acids was as following: protein> polysaccharide and aliphatic.
25970893	8	1	theme	lignin	1353:1358	arg1	all					1360:1362	lignin all	1353:1362	lignin all	1353:1362	Aliphatic, polysaccharide, protein and lignin all were degraded during composting, however, the order of degradation was different between humic and fulvci acids.
25970893	9	2	theme	two-dimensional	1493:1507	arg1	analysis					1521:1528	two-dimensional correlation analysis	1493:1528	two-dimensional correlation analysis	1493:1528	The result from two-dimensional correlation analysis showed that, organic compounds in humic acids were degraded in the following sequence: aliphatic> protein> polysaccharide and lignin, while that in fulvic acids was as following: protein> polysaccharide and aliphatic.
25970893	9	3	theme	following	1597:1605	arg1	sequence					1607:1614	the following sequence	1593:1614	the following sequence: aliphatic> protein> polysaccharide and lignin	1593:1661	The result from two-dimensional correlation analysis showed that, organic compounds in humic acids were degraded in the following sequence: aliphatic> protein> polysaccharide and lignin, while that in fulvic acids was as following: protein> polysaccharide and aliphatic.
25970893	2	4	theme	municipal	347:355	arg1	wastes					363:368	municipal solid wastes	347:368	municipal solid wastes	347:368	In order to explore the composition and evolution mechanism, municipal solid wastes were collected to compost and humic and fulvic acids were obtained from these composted municipal solid wastes.
25970893	9	5	theme	protein>	1628:1635	arg1	polysaccharide					1637:1650	aliphatic> protein> polysaccharide	1617:1650	aliphatic> protein> polysaccharide	1617:1650	The result from two-dimensional correlation analysis showed that, organic compounds in humic acids were degraded in the following sequence: aliphatic> protein> polysaccharide and lignin, while that in fulvic acids was as following: protein> polysaccharide and aliphatic.
25970893	11	6	dep	formation	2172:2180	arg1	mechanism					2182:2190	mechanism	2182:2190	mechanism	2182:2190	It can be concluded that, fourier transform infrared spectra coupled with two-dimensional correlation analysis not only can analyze the function group composition of humic substances, but also can characterize effectively the degradation sequence of these groups and identified the formation mechanism and dynamics of humic substances during composting.
25970893	9	7	theme	correlation	1509:1519	arg1	analysis					1521:1528	two-dimensional correlation analysis	1493:1528	two-dimensional correlation analysis	1493:1528	The result from two-dimensional correlation analysis showed that, organic compounds in humic acids were degraded in the following sequence: aliphatic> protein> polysaccharide and lignin, while that in fulvic acids was as following: protein> polysaccharide and aliphatic.
25970893	4	8	theme	absorbance	794:803	arg1	peaks					805:809	several absorbance peaks	786:809	several absorbance peaks	786:809	The results from fourier transform infrared spectra showed that, the composition of humic acids was complex, and several absorbance peaks were observed at 2917-2924, 2844-2852, 2549, 1662, 1622, 1566, 1454, 1398, 1351, 990-1063, 839 and 711 cm(-1).
25970893	11	9	theme	humic	2208:2212	arg1	substances					2214:2223	humic substances	2208:2223	humic substances	2208:2223	It can be concluded that, fourier transform infrared spectra coupled with two-dimensional correlation analysis not only can analyze the function group composition of humic substances, but also can characterize effectively the degradation sequence of these groups and identified the formation mechanism and dynamics of humic substances during composting.
25970893	11	10	theme	two-dimensional	1964:1978	arg1	analysis					1992:1999	two-dimensional correlation analysis	1964:1999	two-dimensional correlation analysis	1964:1999	It can be concluded that, fourier transform infrared spectra coupled with two-dimensional correlation analysis not only can analyze the function group composition of humic substances, but also can characterize effectively the degradation sequence of these groups and identified the formation mechanism and dynamics of humic substances during composting.
25970893	11	11	dep	fourier	1916:1922	arg1	transform					1924:1932	transform	1924:1932	transform infrared spectra coupled with two-dimensional correlation analysis	1924:1999	It can be concluded that, fourier transform infrared spectra coupled with two-dimensional correlation analysis not only can analyze the function group composition of humic substances, but also can characterize effectively the degradation sequence of these groups and identified the formation mechanism and dynamics of humic substances during composting.
25970893	3	12	theme	fulvic	641:646	arg1	acids					648:652	humic and fulvic acids	631:652	acids	648:652	Furthermore, fourier transform infrared spectra and two-dimensional correlation analysis were applied to study the composition and transformation of humic and fulvic acids during composting.
25970893	11	13	theme	substances	2214:2223	arg1	dynamics					2196:2203	dynamics	2196:2203	dynamics	2196:2203	It can be concluded that, fourier transform infrared spectra coupled with two-dimensional correlation analysis not only can analyze the function group composition of humic substances, but also can characterize effectively the degradation sequence of these groups and identified the formation mechanism and dynamics of humic substances during composting.
25970893	11	13	theme	substances	2214:2223	arg1	formation					2172:2180	the formation mechanism and dynamics	2168:2203	formation	2172:2180	It can be concluded that, fourier transform infrared spectra coupled with two-dimensional correlation analysis not only can analyze the function group composition of humic substances, but also can characterize effectively the degradation sequence of these groups and identified the formation mechanism and dynamics of humic substances during composting.
25970893	3	14	theme	correlation	550:560	arg1	analysis					562:569	two-dimensional correlation analysis	534:569	two-dimensional correlation analysis	534:569	Furthermore, fourier transform infrared spectra and two-dimensional correlation analysis were applied to study the composition and transformation of humic and fulvic acids during composting.
25970893	7	15	theme	protein	1262:1268	arg1	number					1238:1243	a large number	1230:1243	a large number of aliphatic and protein which were hardly detected in fulvic acids	1230:1311	In addition, humic acids comprised a large number of aliphatic and protein which were hardly detected in fulvic acids.
25970893	4	16	theme	humic	757:761	arg1	acids					763:767	humic acids	757:767	humic acids	757:767	The results from fourier transform infrared spectra showed that, the composition of humic acids was complex, and several absorbance peaks were observed at 2917-2924, 2844-2852, 2549, 1662, 1622, 1566, 1454, 1398, 1351, 990-1063, 839 and 711 cm(-1).
25970893	7	17	theme	fulvic	1300:1305	arg1	acids					1307:1311	fulvic acids	1300:1311	fulvic acids	1300:1311	In addition, humic acids comprised a large number of aliphatic and protein which were hardly detected in fulvic acids.
25970893	5	18	located	detected	1017:1024	arg1	cm					1048:1049	1637 and 990 cm	1035:1049	1637 and 990 cm(-1)	1035:1053	Compared to humic acids, the composition of fulvci acids was simple, and only three peaks were detected at 1725, 1637 and 990 cm(-1).
25970893	5	18	located	detected	1017:1024	arg1	1725					1029:1032	1725	1029:1032	1725	1029:1032	Compared to humic acids, the composition of fulvci acids was simple, and only three peaks were detected at 1725, 1637 and 990 cm(-1).
25970893	5	18	located	detected	1017:1024	arg2	peaks					1006:1010	only three peaks	995:1010	only three peaks	995:1010	Compared to humic acids, the composition of fulvci acids was simple, and only three peaks were detected at 1725, 1637 and 990 cm(-1).
25970893	4	19	from	fourier	690:696	arg1	results					677:683	The results	673:683	The results from fourier	673:696	The results from fourier transform infrared spectra showed that, the composition of humic acids was complex, and several absorbance peaks were observed at 2917-2924, 2844-2852, 2549, 1662, 1622, 1566, 1454, 1398, 1351, 990-1063, 839 and 711 cm(-1).
25970893	0	20	theme	humic	118:122	arg1	acids					124:128	humic acids	118:128	humic acids	118:128	[Apply fourier transform infrared spectra coupled with two-dimensional correlation analysis to study the evolution of humic acids during composting].
25970893	4	21	theme	several	786:792	arg1	peaks					805:809	several absorbance peaks	786:809	several absorbance peaks	786:809	The results from fourier transform infrared spectra showed that, the composition of humic acids was complex, and several absorbance peaks were observed at 2917-2924, 2844-2852, 2549, 1662, 1622, 1566, 1454, 1398, 1351, 990-1063, 839 and 711 cm(-1).
25970893	7	22	theme	humic	1208:1212	arg1	acids					1214:1218	humic acids	1208:1218	humic acids	1208:1218	In addition, humic acids comprised a large number of aliphatic and protein which were hardly detected in fulvic acids.
25970893	9	23	from	compounds	1551:1559	arg1	acids					1570:1574	humic acids	1564:1574	humic acids	1564:1574	The result from two-dimensional correlation analysis showed that, organic compounds in humic acids were degraded in the following sequence: aliphatic> protein> polysaccharide and lignin, while that in fulvic acids was as following: protein> polysaccharide and aliphatic.
25970893	3	24	theme	two-dimensional	534:548	arg1	analysis					562:569	two-dimensional correlation analysis	534:569	two-dimensional correlation analysis	534:569	Furthermore, fourier transform infrared spectra and two-dimensional correlation analysis were applied to study the composition and transformation of humic and fulvic acids during composting.
25970893	3	25	dep	composition	597:607	arg1	the					593:595	the	593:595	the	593:595	Furthermore, fourier transform infrared spectra and two-dimensional correlation analysis were applied to study the composition and transformation of humic and fulvic acids during composting.
25970893	11	26	theme	function	2026:2033	arg1	composition					2041:2051	the function group composition	2022:2051	the function group composition of humic substances	2022:2071	It can be concluded that, fourier transform infrared spectra coupled with two-dimensional correlation analysis not only can analyze the function group composition of humic substances, but also can characterize effectively the degradation sequence of these groups and identified the formation mechanism and dynamics of humic substances during composting.
25970893	6	27	theme	fulvic	1113:1118	arg1	acids					1120:1124	both humic and fulvic acids	1098:1124	both humic and fulvic acids	1098:1124	The appearance of these peaks showed that both humic and fulvic acids comprised the benzene originated from lignin and the polysaccharide.
25970893	11	28	theme	group	2035:2039	arg1	composition					2041:2051	the function group composition	2022:2051	the function group composition of humic substances	2022:2071	It can be concluded that, fourier transform infrared spectra coupled with two-dimensional correlation analysis not only can analyze the function group composition of humic substances, but also can characterize effectively the degradation sequence of these groups and identified the formation mechanism and dynamics of humic substances during composting.
25970893	1	29	dep	composition	154:164	arg1	The					150:152	The	150:152	The	150:152	The composition and structure of humic acids formed during composting play an important influence on the quality and mature of compost.
25970893	9	30	theme	organic	1543:1549	arg1	compounds					1551:1559	organic compounds	1543:1559	organic compounds in humic acids	1543:1574	The result from two-dimensional correlation analysis showed that, organic compounds in humic acids were degraded in the following sequence: aliphatic> protein> polysaccharide and lignin, while that in fulvic acids was as following: protein> polysaccharide and aliphatic.
25970893	1	31	theme	humic	183:187	arg1	acids					189:193	humic acids	183:193	humic acids formed during composting	183:218	The composition and structure of humic acids formed during composting play an important influence on the quality and mature of compost.
25970893	0	32	theme	[Apply	0:5	arg1	fourier					7:13	[Apply fourier	0:13	[Apply fourier	0:13	[Apply fourier transform infrared spectra coupled with two-dimensional correlation analysis to study the evolution of humic acids during composting].
25970893	10	33	theme	degradation	1819:1829	arg1	process					1831:1837	the degradation process	1815:1837	the degradation process	1815:1837	A large number of carboxyl, alcohols and ethers were formed during the degradation process, and the carboxyl was transformed into carbonates.
25970893	11	34	theme	humic	2056:2060	arg1	substances					2062:2071	humic substances	2056:2071	humic substances	2056:2071	It can be concluded that, fourier transform infrared spectra coupled with two-dimensional correlation analysis not only can analyze the function group composition of humic substances, but also can characterize effectively the degradation sequence of these groups and identified the formation mechanism and dynamics of humic substances during composting.
25970893	2	35	dep	composition	310:320	arg1	the					306:308	the	306:308	the	306:308	In order to explore the composition and evolution mechanism, municipal solid wastes were collected to compost and humic and fulvic acids were obtained from these composted municipal solid wastes.
25970893	1	36	theme	acids	189:193	arg1	structure					170:178	structure	170:178	structure	170:178	The composition and structure of humic acids formed during composting play an important influence on the quality and mature of compost.
25970893	1	36	theme	acids	189:193	arg1	composition					154:164	composition	154:164	composition	154:164	The composition and structure of humic acids formed during composting play an important influence on the quality and mature of compost.
25970893	1	37	theme	compost	277:283	arg1	quality					255:261	quality	255:261	quality	255:261	The composition and structure of humic acids formed during composting play an important influence on the quality and mature of compost.
25970893	11	38	theme	correlation	1980:1990	arg1	analysis					1992:1999	two-dimensional correlation analysis	1964:1999	two-dimensional correlation analysis	1964:1999	It can be concluded that, fourier transform infrared spectra coupled with two-dimensional correlation analysis not only can analyze the function group composition of humic substances, but also can characterize effectively the degradation sequence of these groups and identified the formation mechanism and dynamics of humic substances during composting.
25970893	2	39	theme	fulvic	410:415	arg1	acids					417:421	humic and fulvic acids	400:421	acids	417:421	In order to explore the composition and evolution mechanism, municipal solid wastes were collected to compost and humic and fulvic acids were obtained from these composted municipal solid wastes.
25970893	9	40	theme	fulvic	1678:1683	arg1	acids					1685:1689	fulvic acids	1678:1689	fulvic acids	1678:1689	The result from two-dimensional correlation analysis showed that, organic compounds in humic acids were degraded in the following sequence: aliphatic> protein> polysaccharide and lignin, while that in fulvic acids was as following: protein> polysaccharide and aliphatic.
25970893	0	41	theme	acids	124:128	arg1	evolution					105:113	the evolution	101:113	the evolution of humic acids	101:128	[Apply fourier transform infrared spectra coupled with two-dimensional correlation analysis to study the evolution of humic acids during composting].
25970893	4	42	theme	acids	763:767	arg1	cm					914:915	observed at 2917-2924, 2844-2852, 2549, 1662, 1622, 1566, 1454, 1398, 1351, 990-1063, 839 and 711 cm(-1)	816:919	observed at 2917-2924, 2844-2852, 2549, 1662, 1622, 1566, 1454, 1398, 1351, 990-1063, 839 and 711 cm(-1)	816:919	The results from fourier transform infrared spectra showed that, the composition of humic acids was complex, and several absorbance peaks were observed at 2917-2924, 2844-2852, 2549, 1662, 1622, 1566, 1454, 1398, 1351, 990-1063, 839 and 711 cm(-1).
25970893	4	42	theme	acids	763:767	arg1	composition					742:752	the composition	738:752	the composition of humic acids	738:767	The results from fourier transform infrared spectra showed that, the composition of humic acids was complex, and several absorbance peaks were observed at 2917-2924, 2844-2852, 2549, 1662, 1622, 1566, 1454, 1398, 1351, 990-1063, 839 and 711 cm(-1).
25970893	4	43	theme	infrared	708:715	arg1	spectra					717:723	infrared spectra	708:723	infrared spectra	708:723	The results from fourier transform infrared spectra showed that, the composition of humic acids was complex, and several absorbance peaks were observed at 2917-2924, 2844-2852, 2549, 1662, 1622, 1566, 1454, 1398, 1351, 990-1063, 839 and 711 cm(-1).
25970893	2	44	theme	solid	468:472	arg1	wastes					474:479	these composted municipal solid wastes	442:479	these composted municipal solid wastes	442:479	In order to explore the composition and evolution mechanism, municipal solid wastes were collected to compost and humic and fulvic acids were obtained from these composted municipal solid wastes.
25970893	11	45	theme	groups	2146:2151	arg1	sequence					2128:2135	the degradation sequence	2112:2135	the degradation sequence of these groups	2112:2151	It can be concluded that, fourier transform infrared spectra coupled with two-dimensional correlation analysis not only can analyze the function group composition of humic substances, but also can characterize effectively the degradation sequence of these groups and identified the formation mechanism and dynamics of humic substances during composting.
25970893	9	46	dep	sequence	1607:1614	arg1	lignin					1656:1661	lignin	1656:1661	lignin	1656:1661	The result from two-dimensional correlation analysis showed that, organic compounds in humic acids were degraded in the following sequence: aliphatic> protein> polysaccharide and lignin, while that in fulvic acids was as following: protein> polysaccharide and aliphatic.
25970893	9	46	dep	sequence	1607:1614	arg1	polysaccharide					1637:1650	aliphatic> protein> polysaccharide	1617:1650	aliphatic> protein> polysaccharide	1617:1650	The result from two-dimensional correlation analysis showed that, organic compounds in humic acids were degraded in the following sequence: aliphatic> protein> polysaccharide and lignin, while that in fulvic acids was as following: protein> polysaccharide and aliphatic.
25970893	6	47	theme	humic	1103:1107	arg1	acids					1120:1124	both humic and fulvic acids	1098:1124	both humic and fulvic acids	1098:1124	The appearance of these peaks showed that both humic and fulvic acids comprised the benzene originated from lignin and the polysaccharide.
25970893	2	48	theme	humic	400:404	arg1	acids					417:421	humic and fulvic acids	400:421	acids	417:421	In order to explore the composition and evolution mechanism, municipal solid wastes were collected to compost and humic and fulvic acids were obtained from these composted municipal solid wastes.
25970893	2	49	theme	municipal	458:466	arg1	wastes					474:479	these composted municipal solid wastes	442:479	these composted municipal solid wastes	442:479	In order to explore the composition and evolution mechanism, municipal solid wastes were collected to compost and humic and fulvic acids were obtained from these composted municipal solid wastes.
25970893	9	50	from	analysis	1521:1528	arg1	result					1481:1486	The result	1477:1486	The result from two-dimensional correlation analysis	1477:1528	The result from two-dimensional correlation analysis showed that, organic compounds in humic acids were degraded in the following sequence: aliphatic> protein> polysaccharide and lignin, while that in fulvic acids was as following: protein> polysaccharide and aliphatic.
25970893	11	51	theme	substances	2062:2071	arg1	composition					2041:2051	the function group composition	2022:2051	the function group composition of humic substances	2022:2071	It can be concluded that, fourier transform infrared spectra coupled with two-dimensional correlation analysis not only can analyze the function group composition of humic substances, but also can characterize effectively the degradation sequence of these groups and identified the formation mechanism and dynamics of humic substances during composting.
25970893	3	52	theme	acids	648:652	arg1	transformation					613:626	transformation	613:626	transformation	613:626	Furthermore, fourier transform infrared spectra and two-dimensional correlation analysis were applied to study the composition and transformation of humic and fulvic acids during composting.
25970893	3	52	theme	acids	648:652	arg1	composition					597:607	composition	597:607	composition	597:607	Furthermore, fourier transform infrared spectra and two-dimensional correlation analysis were applied to study the composition and transformation of humic and fulvic acids during composting.
25970893	4	53	theme	at	825:826	arg1	cm					914:915	observed at 2917-2924, 2844-2852, 2549, 1662, 1622, 1566, 1454, 1398, 1351, 990-1063, 839 and 711 cm(-1)	816:919	observed at 2917-2924, 2844-2852, 2549, 1662, 1622, 1566, 1454, 1398, 1351, 990-1063, 839 and 711 cm(-1)	816:919	The results from fourier transform infrared spectra showed that, the composition of humic acids was complex, and several absorbance peaks were observed at 2917-2924, 2844-2852, 2549, 1662, 1622, 1566, 1454, 1398, 1351, 990-1063, 839 and 711 cm(-1).
25970893	4	53	theme	at	825:826	arg1	composition					742:752	the composition	738:752	the composition of humic acids	738:767	The results from fourier transform infrared spectra showed that, the composition of humic acids was complex, and several absorbance peaks were observed at 2917-2924, 2844-2852, 2549, 1662, 1622, 1566, 1454, 1398, 1351, 990-1063, 839 and 711 cm(-1).
25970893	2	54	theme	composted	448:456	arg1	wastes					474:479	these composted municipal solid wastes	442:479	these composted municipal solid wastes	442:479	In order to explore the composition and evolution mechanism, municipal solid wastes were collected to compost and humic and fulvic acids were obtained from these composted municipal solid wastes.
25970893	8	55	theme	degradation	1419:1429	arg1	order					1410:1414	the order	1406:1414	the order of degradation	1406:1429	Aliphatic, polysaccharide, protein and lignin all were degraded during composting, however, the order of degradation was different between humic and fulvci acids.
25970893	8	55	theme	degradation	1419:1429	arg1	different					1435:1443	different	1435:1443	different	1435:1443	Aliphatic, polysaccharide, protein and lignin all were degraded during composting, however, the order of degradation was different between humic and fulvci acids.
25970893	11	56	theme	infrared	1934:1941	arg1	spectra					1943:1949	infrared spectra	1934:1949	infrared spectra coupled with two-dimensional correlation analysis	1934:1999	It can be concluded that, fourier transform infrared spectra coupled with two-dimensional correlation analysis not only can analyze the function group composition of humic substances, but also can characterize effectively the degradation sequence of these groups and identified the formation mechanism and dynamics of humic substances during composting.
25970893	0	57	theme	correlation	71:81	arg1	analysis					83:90	two-dimensional correlation analysis	55:90	two-dimensional correlation analysis	55:90	[Apply fourier transform infrared spectra coupled with two-dimensional correlation analysis to study the evolution of humic acids during composting].
25970893	7	58	theme	large	1232:1236	arg1	number					1238:1243	a large number	1230:1243	a large number of aliphatic and protein which were hardly detected in fulvic acids	1230:1311	In addition, humic acids comprised a large number of aliphatic and protein which were hardly detected in fulvic acids.
25970893	8	59	theme	humic	1453:1457	arg1	acids					1470:1474	humic and fulvci acids	1453:1474	acids	1470:1474	Aliphatic, polysaccharide, protein and lignin all were degraded during composting, however, the order of degradation was different between humic and fulvci acids.
25970893	7	60	located	detected	1288:1295	arg1	acids					1307:1311	fulvic acids	1300:1311	fulvic acids	1300:1311	In addition, humic acids comprised a large number of aliphatic and protein which were hardly detected in fulvic acids.
25970893	7	60	located	detected	1288:1295	arg2	number					1238:1243	a large number	1230:1243	a large number of aliphatic and protein which were hardly detected in fulvic acids	1230:1311	In addition, humic acids comprised a large number of aliphatic and protein which were hardly detected in fulvic acids.
25970893	10	61	theme	large	1750:1754	arg1	number					1756:1761	A large number	1748:1761	A large number of carboxyl, alcohols and ethers	1748:1794	A large number of carboxyl, alcohols and ethers were formed during the degradation process, and the carboxyl was transformed into carbonates.
25970893	9	62	dep	following	1698:1706	arg1	polysaccharide					1718:1731	protein> polysaccharide	1709:1731	protein> polysaccharide	1709:1731	The result from two-dimensional correlation analysis showed that, organic compounds in humic acids were degraded in the following sequence: aliphatic> protein> polysaccharide and lignin, while that in fulvic acids was as following: protein> polysaccharide and aliphatic.
25970893	9	62	dep	following	1698:1706	arg1	aliphatic					1737:1745	aliphatic	1737:1745	aliphatic	1737:1745	The result from two-dimensional correlation analysis showed that, organic compounds in humic acids were degraded in the following sequence: aliphatic> protein> polysaccharide and lignin, while that in fulvic acids was as following: protein> polysaccharide and aliphatic.
25970893	2	63	theme	evolution	326:334	arg1	mechanism					336:344	the composition and evolution mechanism	306:344	the composition and evolution mechanism	306:344	In order to explore the composition and evolution mechanism, municipal solid wastes were collected to compost and humic and fulvic acids were obtained from these composted municipal solid wastes.
25970893	5	64	theme	humic	934:938	arg1	acids					940:944	humic acids	934:944	humic acids	934:944	Compared to humic acids, the composition of fulvci acids was simple, and only three peaks were detected at 1725, 1637 and 990 cm(-1).
25970893	5	65	theme	fulvci	966:971	arg1	acids					973:977	fulvci acids	966:977	fulvci acids	966:977	Compared to humic acids, the composition of fulvci acids was simple, and only three peaks were detected at 1725, 1637 and 990 cm(-1).
25970893	6	66	theme	peaks	1080:1084	arg1	appearance					1060:1069	The appearance	1056:1069	The appearance of these peaks	1056:1084	The appearance of these peaks showed that both humic and fulvic acids comprised the benzene originated from lignin and the polysaccharide.
25970893	7	67	theme	aliphatic	1248:1256	arg1	number					1238:1243	a large number	1230:1243	a large number of aliphatic and protein which were hardly detected in fulvic acids	1230:1311	In addition, humic acids comprised a large number of aliphatic and protein which were hardly detected in fulvic acids.
25970893	9	68	theme	protein>	1709:1716	arg1	polysaccharide					1718:1731	protein> polysaccharide	1709:1731	protein> polysaccharide	1709:1731	The result from two-dimensional correlation analysis showed that, organic compounds in humic acids were degraded in the following sequence: aliphatic> protein> polysaccharide and lignin, while that in fulvic acids was as following: protein> polysaccharide and aliphatic.
25970893	4	69	dep	cm	914:915	arg1	was					769:771	was	769:771	was	769:771	The results from fourier transform infrared spectra showed that, the composition of humic acids was complex, and several absorbance peaks were observed at 2917-2924, 2844-2852, 2549, 1662, 1622, 1566, 1454, 1398, 1351, 990-1063, 839 and 711 cm(-1).
25970893	4	69	dep	cm	914:915	arg1	complex					773:779	complex	773:779	complex	773:779	The results from fourier transform infrared spectra showed that, the composition of humic acids was complex, and several absorbance peaks were observed at 2917-2924, 2844-2852, 2549, 1662, 1622, 1566, 1454, 1398, 1351, 990-1063, 839 and 711 cm(-1).
25970893	5	70	theme	acids	973:977	arg1	simple					983:988	simple	983:988	simple	983:988	Compared to humic acids, the composition of fulvci acids was simple, and only three peaks were detected at 1725, 1637 and 990 cm(-1).
25970893	5	70	theme	acids	973:977	arg1	composition					951:961	the composition	947:961	the composition of fulvci acids	947:977	Compared to humic acids, the composition of fulvci acids was simple, and only three peaks were detected at 1725, 1637 and 990 cm(-1).
25970893	0	71	dep	transform	15:23	arg1	infrared					25:32	infrared	25:32	transform infrared spectra coupled with two-dimensional correlation analysis to study the evolution of humic acids during composting]	15:147	[Apply fourier transform infrared spectra coupled with two-dimensional correlation analysis to study the evolution of humic acids during composting].
25970893	2	72	theme	composition	310:320	arg1	mechanism					336:344	the composition and evolution mechanism	306:344	the composition and evolution mechanism	306:344	In order to explore the composition and evolution mechanism, municipal solid wastes were collected to compost and humic and fulvic acids were obtained from these composted municipal solid wastes.
25970893	1	73	theme	important	228:236	arg1	influence					238:246	an important influence	225:246	an important influence	225:246	The composition and structure of humic acids formed during composting play an important influence on the quality and mature of compost.
25970893	1	74	dep	quality	255:261	arg1	the					251:253	the	251:253	the	251:253	The composition and structure of humic acids formed during composting play an important influence on the quality and mature of compost.
25970893	4	75	theme	observed	816:823	arg1	cm					914:915	observed at 2917-2924, 2844-2852, 2549, 1662, 1622, 1566, 1454, 1398, 1351, 990-1063, 839 and 711 cm(-1)	816:919	observed at 2917-2924, 2844-2852, 2549, 1662, 1622, 1566, 1454, 1398, 1351, 990-1063, 839 and 711 cm(-1)	816:919	The results from fourier transform infrared spectra showed that, the composition of humic acids was complex, and several absorbance peaks were observed at 2917-2924, 2844-2852, 2549, 1662, 1622, 1566, 1454, 1398, 1351, 990-1063, 839 and 711 cm(-1).
25970893	4	75	theme	observed	816:823	arg1	composition					742:752	the composition	738:752	the composition of humic acids	738:767	The results from fourier transform infrared spectra showed that, the composition of humic acids was complex, and several absorbance peaks were observed at 2917-2924, 2844-2852, 2549, 1662, 1622, 1566, 1454, 1398, 1351, 990-1063, 839 and 711 cm(-1).
25970893	3	76	theme	infrared	513:520	arg1	spectra					522:528	infrared spectra	513:528	infrared spectra	513:528	Furthermore, fourier transform infrared spectra and two-dimensional correlation analysis were applied to study the composition and transformation of humic and fulvic acids during composting.
25970893	11	77	theme	degradation	2116:2126	arg1	sequence					2128:2135	the degradation sequence	2112:2135	the degradation sequence of these groups	2112:2151	It can be concluded that, fourier transform infrared spectra coupled with two-dimensional correlation analysis not only can analyze the function group composition of humic substances, but also can characterize effectively the degradation sequence of these groups and identified the formation mechanism and dynamics of humic substances during composting.
25970893	0	78	theme	two-dimensional	55:69	arg1	analysis					83:90	two-dimensional correlation analysis	55:90	two-dimensional correlation analysis	55:90	[Apply fourier transform infrared spectra coupled with two-dimensional correlation analysis to study the evolution of humic acids during composting].
25970893	10	79	theme	carboxyl	1766:1773	arg1	number					1756:1761	A large number	1748:1761	A large number of carboxyl, alcohols and ethers	1748:1794	A large number of carboxyl, alcohols and ethers were formed during the degradation process, and the carboxyl was transformed into carbonates.
25970893	3	80	theme	humic	631:635	arg1	acids					648:652	humic and fulvic acids	631:652	acids	648:652	Furthermore, fourier transform infrared spectra and two-dimensional correlation analysis were applied to study the composition and transformation of humic and fulvic acids during composting.
25970893	2	81	theme	solid	357:361	arg1	wastes					363:368	municipal solid wastes	347:368	municipal solid wastes	347:368	In order to explore the composition and evolution mechanism, municipal solid wastes were collected to compost and humic and fulvic acids were obtained from these composted municipal solid wastes.
25970893	9	82	theme	humic	1564:1568	arg1	acids					1570:1574	humic acids	1564:1574	humic acids	1564:1574	The result from two-dimensional correlation analysis showed that, organic compounds in humic acids were degraded in the following sequence: aliphatic> protein> polysaccharide and lignin, while that in fulvic acids was as following: protein> polysaccharide and aliphatic.
25970893	8	83	theme	fulvci	1463:1468	arg1	acids					1470:1474	humic and fulvci acids	1453:1474	acids	1470:1474	Aliphatic, polysaccharide, protein and lignin all were degraded during composting, however, the order of degradation was different between humic and fulvci acids.
25439892	0	0	theme	pasting	39:45	arg1	behaviors					59:67	the pasting and thermal behaviors	35:67	the pasting and thermal behaviors of maize starch	35:83	Effects of corn fiber gum (CFG) on the pasting and thermal behaviors of maize starch.
25439892	9	1	theme	granules	1366:1373	arg1	leaching					1346:1353	leaching	1346:1353	leaching out of the granules	1346:1373	Confocal laser scanning microscopy (CLSM) confirmed changes in gels microstructure as starch components tended to be inhibited from leaching out of the granules when CFG was added, and the morphology of starch granule was more compact when CFG was added.
25439892	1	2	theme	food	204:207	arg1	processing					209:218	food processing	204:218	food processing	204:218	Corn fiber gum (CFG) was a novel arabinoxylan hydrocolloid and recent researches showed its considerable potential in food processing.
25439892	3	3	theme	Maize	291:295	arg1	gels					314:317	Maize starch/CFG blend gels	291:317	Maize starch/CFG blend gels	291:317	Maize starch/CFG blend gels were prepared from maize starch suspension mixing with 0.1%, 0.25%, 0.5%, 1.0% (w/w) CFG.
25439892	0	4	from	Effects	0:6	arg1	behaviors					59:67	the pasting and thermal behaviors	35:67	the pasting and thermal behaviors of maize starch	35:83	Effects of corn fiber gum (CFG) on the pasting and thermal behaviors of maize starch.
25439892	7	5	theme	thermal	845:851	arg1	characteristics					853:867	The thermal characteristics	841:867	The thermal characteristics of maize starch/CFG mixtures	841:896	The thermal characteristics of maize starch/CFG mixtures varied insignificantly as determined in DSC heating process.
25439892	3	6	theme	starch/CFG	297:306	arg1	gels					314:317	Maize starch/CFG blend gels	291:317	Maize starch/CFG blend gels	291:317	Maize starch/CFG blend gels were prepared from maize starch suspension mixing with 0.1%, 0.25%, 0.5%, 1.0% (w/w) CFG.
25439892	6	7	theme	gels	775:778	arg1	amylose					751:757	leached amylose	743:757	leached amylose of maize starch gels	743:778	The swelling power and the amount of leached amylose of maize starch gels were reduced as the addition concentration of CFG increased.
25439892	0	8	theme	maize	72:76	arg1	starch					78:83	maize starch	72:83	maize starch	72:83	Effects of corn fiber gum (CFG) on the pasting and thermal behaviors of maize starch.
25439892	6	9	theme	starch	768:773	arg1	gels					775:778	maize starch gels	762:778	maize starch gels	762:778	The swelling power and the amount of leached amylose of maize starch gels were reduced as the addition concentration of CFG increased.
25439892	5	10	theme	final	670:674	arg1	viscosity					676:684	increased final viscosity	660:684	increased final viscosity	660:684	Compared with the reference, CFG addition lowered peak viscosity and breakdown of the composite system, but increased final viscosity in RVA measurement.
25439892	9	11	theme	starch	1300:1305	arg1	components					1307:1316	starch components	1300:1316	starch components	1300:1316	Confocal laser scanning microscopy (CLSM) confirmed changes in gels microstructure as starch components tended to be inhibited from leaching out of the granules when CFG was added, and the morphology of starch granule was more compact when CFG was added.
25439892	6	12	theme	maize	762:766	arg1	gels					775:778	maize starch gels	762:778	maize starch gels	762:778	The swelling power and the amount of leached amylose of maize starch gels were reduced as the addition concentration of CFG increased.
25439892	5	13	theme	increased	660:668	arg1	viscosity					676:684	increased final viscosity	660:684	increased final viscosity	660:684	Compared with the reference, CFG addition lowered peak viscosity and breakdown of the composite system, but increased final viscosity in RVA measurement.
25439892	9	14	theme	scanning	1229:1236	arg1	microscopy					1238:1247	Confocal laser scanning microscopy	1214:1247	Confocal laser scanning microscopy (CLSM)	1214:1254	Confocal laser scanning microscopy (CLSM) confirmed changes in gels microstructure as starch components tended to be inhibited from leaching out of the granules when CFG was added, and the morphology of starch granule was more compact when CFG was added.
25439892	9	14	theme	scanning	1229:1236	arg1	CLSM					1250:1253	CLSM	1250:1253	CLSM	1250:1253	Confocal laser scanning microscopy (CLSM) confirmed changes in gels microstructure as starch components tended to be inhibited from leaching out of the granules when CFG was added, and the morphology of starch granule was more compact when CFG was added.
25439892	9	15	dep	granules	1366:1373	arg1	out					1355:1357	out	1355:1357	out	1355:1357	Confocal laser scanning microscopy (CLSM) confirmed changes in gels microstructure as starch components tended to be inhibited from leaching out of the granules when CFG was added, and the morphology of starch granule was more compact when CFG was added.
25439892	5	16	theme	RVA	689:691	arg1	measurement					693:703	RVA measurement	689:703	RVA measurement	689:703	Compared with the reference, CFG addition lowered peak viscosity and breakdown of the composite system, but increased final viscosity in RVA measurement.
25439892	8	17	theme	loss	1016:1019	arg1	modulus					1021:1027	storage modulus (G') and loss modulus	991:1027	storage modulus (G') and loss modulus (G")	991:1032	Rheological parameters, such as storage modulus (G') and loss modulus (G"), of the maize starches were observed to increase when CFG was present, supporting the hypothesis that the interaction between CFG and amylose could happen in the composite system.
25439892	8	17	theme	loss	1016:1019	arg1	"					1031:1031	G"	1030:1031	G"	1030:1031	Rheological parameters, such as storage modulus (G') and loss modulus (G"), of the maize starches were observed to increase when CFG was present, supporting the hypothesis that the interaction between CFG and amylose could happen in the composite system.
25439892	2	18	theme	maize	256:260	arg1	interactions					240:251	the interactions	236:251	the interactions of maize starch and CFG	236:275	In this study, the interactions of maize starch and CFG were studied.
25439892	8	19	theme	modulus	999:1005	arg1	modulus					1021:1027	storage modulus (G') and loss modulus	991:1027	storage modulus (G') and loss modulus (G")	991:1032	Rheological parameters, such as storage modulus (G') and loss modulus (G"), of the maize starches were observed to increase when CFG was present, supporting the hypothesis that the interaction between CFG and amylose could happen in the composite system.
25439892	8	19	theme	modulus	999:1005	arg1	"					1031:1031	G"	1030:1031	G"	1030:1031	Rheological parameters, such as storage modulus (G') and loss modulus (G"), of the maize starches were observed to increase when CFG was present, supporting the hypothesis that the interaction between CFG and amylose could happen in the composite system.
25439892	1	20	theme	novel	113:117	arg1	gum					97:99	Corn fiber gum	86:99	Corn fiber gum (CFG)	86:105	Corn fiber gum (CFG) was a novel arabinoxylan hydrocolloid and recent researches showed its considerable potential in food processing.
25439892	1	20	theme	novel	113:117	arg1	hydrocolloid					132:143	a novel arabinoxylan hydrocolloid	111:143	a novel arabinoxylan hydrocolloid	111:143	Corn fiber gum (CFG) was a novel arabinoxylan hydrocolloid and recent researches showed its considerable potential in food processing.
25439892	8	21	theme	storage	991:997	arg1	G					1008:1008	G'	1008:1009	G'	1008:1009	Rheological parameters, such as storage modulus (G') and loss modulus (G"), of the maize starches were observed to increase when CFG was present, supporting the hypothesis that the interaction between CFG and amylose could happen in the composite system.
25439892	8	21	theme	storage	991:997	arg1	modulus					999:1005	storage modulus	991:1005	storage modulus (G')	991:1010	Rheological parameters, such as storage modulus (G') and loss modulus (G"), of the maize starches were observed to increase when CFG was present, supporting the hypothesis that the interaction between CFG and amylose could happen in the composite system.
25439892	4	22	theme	thermal	425:431	arg1	properties					433:442	The pasting and thermal properties	409:442	The pasting and thermal properties	409:442	The pasting and thermal properties, rheological properties, microstructure, leached amylose and swelling power characteristics were evaluated.
25439892	8	23	theme	Rheological	959:969	arg1	modulus					1021:1027	storage modulus (G') and loss modulus	991:1027	storage modulus (G') and loss modulus (G")	991:1032	Rheological parameters, such as storage modulus (G') and loss modulus (G"), of the maize starches were observed to increase when CFG was present, supporting the hypothesis that the interaction between CFG and amylose could happen in the composite system.
25439892	8	23	theme	Rheological	959:969	arg1	parameters					971:980	Rheological parameters	959:980	Rheological parameters	959:980	Rheological parameters, such as storage modulus (G') and loss modulus (G"), of the maize starches were observed to increase when CFG was present, supporting the hypothesis that the interaction between CFG and amylose could happen in the composite system.
25439892	8	24	theme	composite	1196:1204	arg1	system					1206:1211	the composite system	1192:1211	the composite system	1192:1211	Rheological parameters, such as storage modulus (G') and loss modulus (G"), of the maize starches were observed to increase when CFG was present, supporting the hypothesis that the interaction between CFG and amylose could happen in the composite system.
25439892	8	25	theme	starches	1048:1055	arg1	modulus					1021:1027	storage modulus (G') and loss modulus	991:1027	storage modulus (G') and loss modulus (G")	991:1032	Rheological parameters, such as storage modulus (G') and loss modulus (G"), of the maize starches were observed to increase when CFG was present, supporting the hypothesis that the interaction between CFG and amylose could happen in the composite system.
25439892	8	25	theme	starches	1048:1055	arg1	parameters					971:980	Rheological parameters	959:980	Rheological parameters	959:980	Rheological parameters, such as storage modulus (G') and loss modulus (G"), of the maize starches were observed to increase when CFG was present, supporting the hypothesis that the interaction between CFG and amylose could happen in the composite system.
25439892	1	26	theme	arabinoxylan	119:130	arg1	gum					97:99	Corn fiber gum	86:99	Corn fiber gum (CFG)	86:105	Corn fiber gum (CFG) was a novel arabinoxylan hydrocolloid and recent researches showed its considerable potential in food processing.
25439892	1	26	theme	arabinoxylan	119:130	arg1	hydrocolloid					132:143	a novel arabinoxylan hydrocolloid	111:143	a novel arabinoxylan hydrocolloid	111:143	Corn fiber gum (CFG) was a novel arabinoxylan hydrocolloid and recent researches showed its considerable potential in food processing.
25439892	3	27	theme	blend	308:312	arg1	gels					314:317	Maize starch/CFG blend gels	291:317	Maize starch/CFG blend gels	291:317	Maize starch/CFG blend gels were prepared from maize starch suspension mixing with 0.1%, 0.25%, 0.5%, 1.0% (w/w) CFG.
25439892	8	28	theme	maize	1042:1046	arg1	starches					1048:1055	the maize starches	1038:1055	the maize starches	1038:1055	Rheological parameters, such as storage modulus (G') and loss modulus (G"), of the maize starches were observed to increase when CFG was present, supporting the hypothesis that the interaction between CFG and amylose could happen in the composite system.
25439892	4	29	theme	power	514:518	arg1	characteristics					520:534	swelling power characteristics	505:534	swelling power characteristics	505:534	The pasting and thermal properties, rheological properties, microstructure, leached amylose and swelling power characteristics were evaluated.
25439892	0	30	theme	corn	11:14	arg1	CFG					27:29	CFG	27:29	CFG	27:29	Effects of corn fiber gum (CFG) on the pasting and thermal behaviors of maize starch.
25439892	0	30	theme	corn	11:14	arg1	gum					22:24	corn fiber gum	11:24	corn fiber gum (CFG)	11:30	Effects of corn fiber gum (CFG) on the pasting and thermal behaviors of maize starch.
25439892	0	31	theme	starch	78:83	arg1	behaviors					59:67	the pasting and thermal behaviors	35:67	the pasting and thermal behaviors of maize starch	35:83	Effects of corn fiber gum (CFG) on the pasting and thermal behaviors of maize starch.
25439892	6	32	theme	amylose	751:757	arg1	power					719:723	The swelling power	706:723	The swelling power	706:723	The swelling power and the amount of leached amylose of maize starch gels were reduced as the addition concentration of CFG increased.
25439892	6	32	theme	amylose	751:757	arg1	amount					733:738	the amount	729:738	the amount of leached amylose of maize starch gels	729:778	The swelling power and the amount of leached amylose of maize starch gels were reduced as the addition concentration of CFG increased.
25439892	6	32	theme	amylose	751:757	arg1	amylose					751:757	leached amylose	743:757	leached amylose of maize starch gels	743:778	The swelling power and the amount of leached amylose of maize starch gels were reduced as the addition concentration of CFG increased.
25439892	4	33	theme	swelling	505:512	arg1	characteristics					520:534	swelling power characteristics	505:534	swelling power characteristics	505:534	The pasting and thermal properties, rheological properties, microstructure, leached amylose and swelling power characteristics were evaluated.
25439892	3	34	theme	starch	344:349	arg1	suspension					351:360	maize starch suspension	338:360	maize starch suspension mixing with 0.1%, 0.25%, 0.5%, 1.0% (w/w) CFG	338:406	Maize starch/CFG blend gels were prepared from maize starch suspension mixing with 0.1%, 0.25%, 0.5%, 1.0% (w/w) CFG.
25439892	3	35	dep	%	396:396	arg1	CFG					404:406	CFG	404:406	1.0% (w/w) CFG	393:406	Maize starch/CFG blend gels were prepared from maize starch suspension mixing with 0.1%, 0.25%, 0.5%, 1.0% (w/w) CFG.
25439892	5	36	theme	peak	602:605	arg1	viscosity					607:615	peak viscosity	602:615	peak viscosity	602:615	Compared with the reference, CFG addition lowered peak viscosity and breakdown of the composite system, but increased final viscosity in RVA measurement.
25439892	6	37	theme	leached	743:749	arg1	amylose					751:757	leached amylose	743:757	leached amylose of maize starch gels	743:778	The swelling power and the amount of leached amylose of maize starch gels were reduced as the addition concentration of CFG increased.
25439892	1	38	theme	recent	149:154	arg1	researches					156:165	recent researches	149:165	recent researches	149:165	Corn fiber gum (CFG) was a novel arabinoxylan hydrocolloid and recent researches showed its considerable potential in food processing.
25439892	0	39	theme	gum	22:24	arg1	Effects					0:6	Effects	0:6	Effects of corn fiber gum (CFG) on the pasting and thermal behaviors of maize starch	0:83	Effects of corn fiber gum (CFG) on the pasting and thermal behaviors of maize starch.
25439892	6	40	theme	CFG	826:828	arg1	concentration					809:821	the addition concentration	796:821	the addition concentration of CFG	796:828	The swelling power and the amount of leached amylose of maize starch gels were reduced as the addition concentration of CFG increased.
25439892	4	41	theme	rheological	445:455	arg1	properties					457:466	rheological properties	445:466	rheological properties	445:466	The pasting and thermal properties, rheological properties, microstructure, leached amylose and swelling power characteristics were evaluated.
25439892	0	42	theme	fiber	16:20	arg1	CFG					27:29	CFG	27:29	CFG	27:29	Effects of corn fiber gum (CFG) on the pasting and thermal behaviors of maize starch.
25439892	0	42	theme	fiber	16:20	arg1	gum					22:24	corn fiber gum	11:24	corn fiber gum (CFG)	11:30	Effects of corn fiber gum (CFG) on the pasting and thermal behaviors of maize starch.
25439892	9	43	theme	Confocal	1214:1221	arg1	microscopy					1238:1247	Confocal laser scanning microscopy	1214:1247	Confocal laser scanning microscopy (CLSM)	1214:1254	Confocal laser scanning microscopy (CLSM) confirmed changes in gels microstructure as starch components tended to be inhibited from leaching out of the granules when CFG was added, and the morphology of starch granule was more compact when CFG was added.
25439892	9	43	theme	Confocal	1214:1221	arg1	CLSM					1250:1253	CLSM	1250:1253	CLSM	1250:1253	Confocal laser scanning microscopy (CLSM) confirmed changes in gels microstructure as starch components tended to be inhibited from leaching out of the granules when CFG was added, and the morphology of starch granule was more compact when CFG was added.
25439892	8	44	theme	G	1030:1030	arg1	modulus					1021:1027	storage modulus (G') and loss modulus	991:1027	storage modulus (G') and loss modulus (G")	991:1032	Rheological parameters, such as storage modulus (G') and loss modulus (G"), of the maize starches were observed to increase when CFG was present, supporting the hypothesis that the interaction between CFG and amylose could happen in the composite system.
25439892	8	44	theme	G	1030:1030	arg1	"					1031:1031	G"	1030:1031	G"	1030:1031	Rheological parameters, such as storage modulus (G') and loss modulus (G"), of the maize starches were observed to increase when CFG was present, supporting the hypothesis that the interaction between CFG and amylose could happen in the composite system.
25439892	7	45	theme	DSC	938:940	arg1	process					950:956	DSC heating process	938:956	DSC heating process	938:956	The thermal characteristics of maize starch/CFG mixtures varied insignificantly as determined in DSC heating process.
25439892	9	46	theme	granule	1424:1430	arg1	compact					1441:1447	compact	1441:1447	compact	1441:1447	Confocal laser scanning microscopy (CLSM) confirmed changes in gels microstructure as starch components tended to be inhibited from leaching out of the granules when CFG was added, and the morphology of starch granule was more compact when CFG was added.
25439892	9	46	theme	granule	1424:1430	arg1	morphology					1403:1412	the morphology	1399:1412	the morphology of starch granule	1399:1430	Confocal laser scanning microscopy (CLSM) confirmed changes in gels microstructure as starch components tended to be inhibited from leaching out of the granules when CFG was added, and the morphology of starch granule was more compact when CFG was added.
25439892	3	47	theme	maize	338:342	arg1	suspension					351:360	maize starch suspension	338:360	maize starch suspension mixing with 0.1%, 0.25%, 0.5%, 1.0% (w/w) CFG	338:406	Maize starch/CFG blend gels were prepared from maize starch suspension mixing with 0.1%, 0.25%, 0.5%, 1.0% (w/w) CFG.
25439892	4	48	theme	pasting	413:419	arg1	properties					433:442	The pasting and thermal properties	409:442	The pasting and thermal properties	409:442	The pasting and thermal properties, rheological properties, microstructure, leached amylose and swelling power characteristics were evaluated.
25439892	7	49	theme	heating	942:948	arg1	process					950:956	DSC heating process	938:956	DSC heating process	938:956	The thermal characteristics of maize starch/CFG mixtures varied insignificantly as determined in DSC heating process.
25439892	9	50	dep	gels	1277:1280	arg1	microstructure					1282:1295	microstructure	1282:1295	microstructure	1282:1295	Confocal laser scanning microscopy (CLSM) confirmed changes in gels microstructure as starch components tended to be inhibited from leaching out of the granules when CFG was added, and the morphology of starch granule was more compact when CFG was added.
25439892	7	51	theme	mixtures	889:896	arg1	characteristics					853:867	The thermal characteristics	841:867	The thermal characteristics of maize starch/CFG mixtures	841:896	The thermal characteristics of maize starch/CFG mixtures varied insignificantly as determined in DSC heating process.
25439892	6	52	theme	addition	800:807	arg1	concentration					809:821	the addition concentration	796:821	the addition concentration of CFG	796:828	The swelling power and the amount of leached amylose of maize starch gels were reduced as the addition concentration of CFG increased.
25439892	9	53	theme	starch	1417:1422	arg1	granule					1424:1430	starch granule	1417:1430	starch granule	1417:1430	Confocal laser scanning microscopy (CLSM) confirmed changes in gels microstructure as starch components tended to be inhibited from leaching out of the granules when CFG was added, and the morphology of starch granule was more compact when CFG was added.
25439892	1	54	theme	Corn	86:89	arg1	CFG					102:104	CFG	102:104	CFG	102:104	Corn fiber gum (CFG) was a novel arabinoxylan hydrocolloid and recent researches showed its considerable potential in food processing.
25439892	1	54	theme	Corn	86:89	arg1	hydrocolloid					132:143	a novel arabinoxylan hydrocolloid	111:143	a novel arabinoxylan hydrocolloid	111:143	Corn fiber gum (CFG) was a novel arabinoxylan hydrocolloid and recent researches showed its considerable potential in food processing.
25439892	1	54	theme	Corn	86:89	arg1	gum					97:99	Corn fiber gum	86:99	Corn fiber gum (CFG)	86:105	Corn fiber gum (CFG) was a novel arabinoxylan hydrocolloid and recent researches showed its considerable potential in food processing.
25439892	4	55	theme	leached	485:491	arg1	amylose					493:499	leached amylose	485:499	leached amylose	485:499	The pasting and thermal properties, rheological properties, microstructure, leached amylose and swelling power characteristics were evaluated.
25439892	5	56	theme	CFG	581:583	arg1	addition					585:592	CFG addition	581:592	CFG addition	581:592	Compared with the reference, CFG addition lowered peak viscosity and breakdown of the composite system, but increased final viscosity in RVA measurement.
25439892	7	57	theme	maize	872:876	arg1	mixtures					889:896	maize starch/CFG mixtures	872:896	maize starch/CFG mixtures	872:896	The thermal characteristics of maize starch/CFG mixtures varied insignificantly as determined in DSC heating process.
25439892	1	58	theme	fiber	91:95	arg1	CFG					102:104	CFG	102:104	CFG	102:104	Corn fiber gum (CFG) was a novel arabinoxylan hydrocolloid and recent researches showed its considerable potential in food processing.
25439892	1	58	theme	fiber	91:95	arg1	hydrocolloid					132:143	a novel arabinoxylan hydrocolloid	111:143	a novel arabinoxylan hydrocolloid	111:143	Corn fiber gum (CFG) was a novel arabinoxylan hydrocolloid and recent researches showed its considerable potential in food processing.
25439892	1	58	theme	fiber	91:95	arg1	gum					97:99	Corn fiber gum	86:99	Corn fiber gum (CFG)	86:105	Corn fiber gum (CFG) was a novel arabinoxylan hydrocolloid and recent researches showed its considerable potential in food processing.
25439892	2	59	dep	maize	256:260	arg1	CFG					273:275	CFG	273:275	CFG	273:275	In this study, the interactions of maize starch and CFG were studied.
25439892	2	59	dep	maize	256:260	arg1	starch					262:267	starch	262:267	starch	262:267	In this study, the interactions of maize starch and CFG were studied.
25439892	2	59	dep	maize	256:260	arg1	maize					256:260	maize starch and CFG	256:275	maize starch and CFG	256:275	In this study, the interactions of maize starch and CFG were studied.
25439892	5	60	theme	composite	638:646	arg1	system					648:653	the composite system	634:653	the composite system	634:653	Compared with the reference, CFG addition lowered peak viscosity and breakdown of the composite system, but increased final viscosity in RVA measurement.
25439892	6	61	theme	swelling	710:717	arg1	power					719:723	The swelling power	706:723	The swelling power	706:723	The swelling power and the amount of leached amylose of maize starch gels were reduced as the addition concentration of CFG increased.
25439892	7	62	theme	starch/CFG	878:887	arg1	mixtures					889:896	maize starch/CFG mixtures	872:896	maize starch/CFG mixtures	872:896	The thermal characteristics of maize starch/CFG mixtures varied insignificantly as determined in DSC heating process.
25439892	1	63	theme	considerable	178:189	arg1	potential					191:199	its considerable potential	174:199	its considerable potential	174:199	Corn fiber gum (CFG) was a novel arabinoxylan hydrocolloid and recent researches showed its considerable potential in food processing.
25439892	5	64	theme	system	648:653	arg1	viscosity					676:684	increased final viscosity	660:684	increased final viscosity	660:684	Compared with the reference, CFG addition lowered peak viscosity and breakdown of the composite system, but increased final viscosity in RVA measurement.
25439892	5	64	theme	system	648:653	arg1	breakdown					621:629	breakdown	621:629	breakdown of the composite system	621:653	Compared with the reference, CFG addition lowered peak viscosity and breakdown of the composite system, but increased final viscosity in RVA measurement.
25439892	5	64	theme	system	648:653	arg1	viscosity					607:615	peak viscosity	602:615	peak viscosity	602:615	Compared with the reference, CFG addition lowered peak viscosity and breakdown of the composite system, but increased final viscosity in RVA measurement.
25439892	9	65	from	changes	1266:1272	arg1	gels					1277:1280	gels	1277:1280	gels microstructure as starch components tended to be inhibited from leaching out of the granules when CFG was added, and the morphology of starch granule was more compact when CFG was added	1277:1466	Confocal laser scanning microscopy (CLSM) confirmed changes in gels microstructure as starch components tended to be inhibited from leaching out of the granules when CFG was added, and the morphology of starch granule was more compact when CFG was added.
25439892	9	66	theme	laser	1223:1227	arg1	microscopy					1238:1247	Confocal laser scanning microscopy	1214:1247	Confocal laser scanning microscopy (CLSM)	1214:1254	Confocal laser scanning microscopy (CLSM) confirmed changes in gels microstructure as starch components tended to be inhibited from leaching out of the granules when CFG was added, and the morphology of starch granule was more compact when CFG was added.
25439892	9	66	theme	laser	1223:1227	arg1	CLSM					1250:1253	CLSM	1250:1253	CLSM	1250:1253	Confocal laser scanning microscopy (CLSM) confirmed changes in gels microstructure as starch components tended to be inhibited from leaching out of the granules when CFG was added, and the morphology of starch granule was more compact when CFG was added.
25439892	0	67	theme	thermal	51:57	arg1	behaviors					59:67	the pasting and thermal behaviors	35:67	the pasting and thermal behaviors of maize starch	35:83	Effects of corn fiber gum (CFG) on the pasting and thermal behaviors of maize starch.
24423101	0	0	theme	inbred	90:95	arg1	lines					97:101	Brachypodium distachyon inbred lines	66:101	Brachypodium distachyon inbred lines	66:101	Selection and phenotypic characterization of a core collection of Brachypodium distachyon inbred lines.
24423101	6	1	from	differences	860:870	arg1	composition					885:895	cell wall composition	875:895	cell wall composition	875:895	We also surveyed differences in cell wall composition using near infrared spectroscopy (NIR) and comprehensive microarray polymer profiling (CoMPP).
24423101	5	2	theme	flowering	810:818	arg1	time					820:823	flowering time	810:823	flowering time	810:823	Phenotypic characters examined included plant height, growth habit, stem density, flowering time, and seed weight.
24423101	6	3	theme	microarray	954:963	arg1	profiling					973:981	comprehensive microarray polymer profiling	940:981	comprehensive microarray polymer profiling (CoMPP)	940:989	We also surveyed differences in cell wall composition using near infrared spectroscopy (NIR) and comprehensive microarray polymer profiling (CoMPP).
24423101	6	3	theme	microarray	954:963	arg1	CoMPP					984:988	CoMPP	984:988	CoMPP	984:988	We also surveyed differences in cell wall composition using near infrared spectroscopy (NIR) and comprehensive microarray polymer profiling (CoMPP).
24423101	0	4	theme	distachyon	79:88	arg1	lines					97:101	Brachypodium distachyon inbred lines	66:101	Brachypodium distachyon inbred lines	66:101	Selection and phenotypic characterization of a core collection of Brachypodium distachyon inbred lines.
24423101	6	5	theme	wall	880:883	arg1	composition					885:895	cell wall composition	875:895	cell wall composition	875:895	We also surveyed differences in cell wall composition using near infrared spectroscopy (NIR) and comprehensive microarray polymer profiling (CoMPP).
24423101	4	6	from	diversity	522:530	arg1	collection					543:552	a large collection	535:552	a large collection of inbred lines	535:568	RESULTS We surveyed the phenotypic diversity in a large collection of inbred lines and then selected a core collection of lines for more detailed analysis with an emphasis on traits relevant to the use of grasses as biofuel and grain crops.
24423101	7	7	from	variation	1167:1175	arg1	density					1085:1091	stem density	1080:1091	stem density	1080:1091	In all cases, we observed extensive natural variation including a two-fold variation in stem density, four-fold variation in ferulic acid bound to hemicellulose, and 1.7-fold variation in seed mass.
24423101	7	7	from	variation	1167:1175	arg1	mass					1185:1188	seed mass	1180:1188	seed mass	1180:1188	In all cases, we observed extensive natural variation including a two-fold variation in stem density, four-fold variation in ferulic acid bound to hemicellulose, and 1.7-fold variation in seed mass.
24423101	7	7	from	variation	1167:1175	arg1	acid					1125:1128	ferulic acid	1117:1128	ferulic acid bound to hemicellulose	1117:1151	In all cases, we observed extensive natural variation including a two-fold variation in stem density, four-fold variation in ferulic acid bound to hemicellulose, and 1.7-fold variation in seed mass.
24423101	7	8	theme	1.7-fold	1158:1165	arg1	variation					1167:1175	1.7-fold variation	1158:1175	1.7-fold variation in seed mass	1158:1188	In all cases, we observed extensive natural variation including a two-fold variation in stem density, four-fold variation in ferulic acid bound to hemicellulose, and 1.7-fold variation in seed mass.
24423101	6	9	theme	cell	875:878	arg1	composition					885:895	cell wall composition	875:895	cell wall composition	875:895	We also surveyed differences in cell wall composition using near infrared spectroscopy (NIR) and comprehensive microarray polymer profiling (CoMPP).
24423101	8	10	theme	basis	1320:1324	arg1	investigations					1290:1303	future investigations	1283:1303	future investigations of the genetic basis of the observed phenotypic variation	1283:1361	CONCLUSION These characterizations can provide the criteria for selecting diverse lines for future investigations of the genetic basis of the observed phenotypic variation.
24423101	8	11	dep	CONCLUSION	1191:1200	arg1	provide					1230:1236	provide	1230:1236	can provide the criteria for selecting diverse lines for future investigations of the genetic basis of the observed phenotypic variation	1226:1361	CONCLUSION These characterizations can provide the criteria for selecting diverse lines for future investigations of the genetic basis of the observed phenotypic variation.
24423101	6	12	theme	polymer	965:971	arg1	profiling					973:981	comprehensive microarray polymer profiling	940:981	comprehensive microarray polymer profiling (CoMPP)	940:989	We also surveyed differences in cell wall composition using near infrared spectroscopy (NIR) and comprehensive microarray polymer profiling (CoMPP).
24423101	6	12	theme	polymer	965:971	arg1	CoMPP					984:988	CoMPP	984:988	CoMPP	984:988	We also surveyed differences in cell wall composition using near infrared spectroscopy (NIR) and comprehensive microarray polymer profiling (CoMPP).
24423101	0	13	theme	lines	97:101	arg1	collection					52:61	a core collection	45:61	a core collection of Brachypodium distachyon inbred lines	45:101	Selection and phenotypic characterization of a core collection of Brachypodium distachyon inbred lines.
24423101	5	14	theme	stem	796:799	arg1	density					801:807	stem density	796:807	stem density	796:807	Phenotypic characters examined included plant height, growth habit, stem density, flowering time, and seed weight.
24423101	7	15	theme	stem	1080:1083	arg1	density					1085:1091	stem density	1080:1091	stem density	1080:1091	In all cases, we observed extensive natural variation including a two-fold variation in stem density, four-fold variation in ferulic acid bound to hemicellulose, and 1.7-fold variation in seed mass.
24423101	1	16	used	used	171:174	arg2	distachyon					144:153	The model grass Brachypodium distachyon	115:153	The model grass Brachypodium distachyon	115:153	BACKGROUND The model grass Brachypodium distachyon is increasingly used to study various aspects of grass biology.
24423101	4	17	theme	core	590:593	arg1	collection					595:604	a core collection	588:604	a core collection of lines	588:613	RESULTS We surveyed the phenotypic diversity in a large collection of inbred lines and then selected a core collection of lines for more detailed analysis with an emphasis on traits relevant to the use of grasses as biofuel and grain crops.
24423101	8	18	theme	diverse	1265:1271	arg1	lines					1273:1277	diverse lines	1265:1277	diverse lines	1265:1277	CONCLUSION These characterizations can provide the criteria for selecting diverse lines for future investigations of the genetic basis of the observed phenotypic variation.
24423101	7	19	theme	two-fold	1058:1065	arg1	variation					1067:1075	a two-fold variation	1056:1075	a two-fold variation in stem density	1056:1091	In all cases, we observed extensive natural variation including a two-fold variation in stem density, four-fold variation in ferulic acid bound to hemicellulose, and 1.7-fold variation in seed mass.
24423101	4	20	theme	detailed	624:631	arg1	analysis					633:640	more detailed analysis	619:640	more detailed analysis with an emphasis on traits relevant to the use of grasses as biofuel and grain crops	619:725	RESULTS We surveyed the phenotypic diversity in a large collection of inbred lines and then selected a core collection of lines for more detailed analysis with an emphasis on traits relevant to the use of grasses as biofuel and grain crops.
24423101	3	21	theme	inquiry	438:444	arg1	areas					429:433	many areas	424:433	many areas	424:433	The natural variation in this collection can serve as a powerful experimental tool for many areas of inquiry, including investigating biomass traits.
24423101	3	21	theme	inquiry	438:444	arg1	traits					479:484	investigating biomass traits	457:484	investigating biomass traits	457:484	The natural variation in this collection can serve as a powerful experimental tool for many areas of inquiry, including investigating biomass traits.
24423101	5	22	theme	Phenotypic	728:737	arg1	characters					739:748	Phenotypic characters	728:748	Phenotypic characters examined	728:757	Phenotypic characters examined included plant height, growth habit, stem density, flowering time, and seed weight.
24423101	2	23	theme	germplasm	281:289	arg1	collection					253:262	A large and genotypically diverse collection	219:262	A large and genotypically diverse collection of B. distachyon germplasm	219:289	A large and genotypically diverse collection of B. distachyon germplasm has been assembled by the research community.
24423101	1	24	theme	various	185:191	arg1	aspects					193:199	various aspects	185:199	various aspects of grass biology	185:216	BACKGROUND The model grass Brachypodium distachyon is increasingly used to study various aspects of grass biology.
24423101	5	25	theme	plant	768:772	arg1	height					774:779	plant height	768:779	plant height	768:779	Phenotypic characters examined included plant height, growth habit, stem density, flowering time, and seed weight.
24423101	7	26	theme	seed	1180:1183	arg1	mass					1185:1188	seed mass	1180:1188	seed mass	1180:1188	In all cases, we observed extensive natural variation including a two-fold variation in stem density, four-fold variation in ferulic acid bound to hemicellulose, and 1.7-fold variation in seed mass.
24423101	3	27	theme	natural	341:347	arg1	tool					415:418	a powerful experimental tool	391:418	a powerful experimental tool for many areas of inquiry, including investigating biomass traits	391:484	The natural variation in this collection can serve as a powerful experimental tool for many areas of inquiry, including investigating biomass traits.
24423101	3	27	theme	natural	341:347	arg1	variation					349:357	The natural variation	337:357	The natural variation in this collection	337:376	The natural variation in this collection can serve as a powerful experimental tool for many areas of inquiry, including investigating biomass traits.
24423101	8	28	theme	phenotypic	1342:1351	arg1	variation					1353:1361	the observed phenotypic variation	1329:1361	the observed phenotypic variation	1329:1361	CONCLUSION These characterizations can provide the criteria for selecting diverse lines for future investigations of the genetic basis of the observed phenotypic variation.
24423101	4	29	from	emphasis	650:657	arg1	traits					662:667	traits	662:667	traits relevant to the use of grasses as biofuel and grain crops	662:725	RESULTS We surveyed the phenotypic diversity in a large collection of inbred lines and then selected a core collection of lines for more detailed analysis with an emphasis on traits relevant to the use of grasses as biofuel and grain crops.
24423101	4	30	theme	relevant	669:676	arg1	traits					662:667	traits	662:667	traits relevant to the use of grasses as biofuel and grain crops	662:725	RESULTS We surveyed the phenotypic diversity in a large collection of inbred lines and then selected a core collection of lines for more detailed analysis with an emphasis on traits relevant to the use of grasses as biofuel and grain crops.
24423101	3	31	theme	powerful	393:400	arg1	tool					415:418	a powerful experimental tool	391:418	a powerful experimental tool for many areas of inquiry, including investigating biomass traits	391:484	The natural variation in this collection can serve as a powerful experimental tool for many areas of inquiry, including investigating biomass traits.
24423101	3	31	theme	powerful	393:400	arg1	variation					349:357	The natural variation	337:357	The natural variation in this collection	337:376	The natural variation in this collection can serve as a powerful experimental tool for many areas of inquiry, including investigating biomass traits.
24423101	4	32	theme	lines	564:568	arg1	collection					543:552	a large collection	535:552	a large collection of inbred lines	535:568	RESULTS We surveyed the phenotypic diversity in a large collection of inbred lines and then selected a core collection of lines for more detailed analysis with an emphasis on traits relevant to the use of grasses as biofuel and grain crops.
24423101	5	33	theme	seed	830:833	arg1	weight					835:840	seed weight	830:840	seed weight	830:840	Phenotypic characters examined included plant height, growth habit, stem density, flowering time, and seed weight.
24423101	2	34	theme	research	317:324	arg1	community					326:334	the research community	313:334	the research community	313:334	A large and genotypically diverse collection of B. distachyon germplasm has been assembled by the research community.
24423101	4	35	theme	grain	715:719	arg1	crops					721:725	grain crops	715:725	grain crops	715:725	RESULTS We surveyed the phenotypic diversity in a large collection of inbred lines and then selected a core collection of lines for more detailed analysis with an emphasis on traits relevant to the use of grasses as biofuel and grain crops.
24423101	7	36	theme	natural	1028:1034	arg1	variation					1036:1044	extensive natural variation	1018:1044	extensive natural variation including a two-fold variation in stem density, four-fold variation in ferulic acid bound to hemicellulose, and 1.7-fold variation in seed mass	1018:1188	In all cases, we observed extensive natural variation including a two-fold variation in stem density, four-fold variation in ferulic acid bound to hemicellulose, and 1.7-fold variation in seed mass.
24423101	5	37	theme	growth	782:787	arg1	habit					789:793	growth habit	782:793	growth habit	782:793	Phenotypic characters examined included plant height, growth habit, stem density, flowering time, and seed weight.
24423101	3	38	theme	biomass	471:477	arg1	traits					479:484	investigating biomass traits	457:484	investigating biomass traits	457:484	The natural variation in this collection can serve as a powerful experimental tool for many areas of inquiry, including investigating biomass traits.
24423101	0	39	theme	phenotypic	14:23	arg1	characterization					25:40	phenotypic characterization	14:40	phenotypic characterization	14:40	Selection and phenotypic characterization of a core collection of Brachypodium distachyon inbred lines.
24423101	4	40	dep	RESULTS	487:493	arg1	selected					579:586	selected	579:586	selected a core collection of lines for more detailed analysis with an emphasis on traits relevant to the use of grasses as biofuel and grain crops	579:725	RESULTS We surveyed the phenotypic diversity in a large collection of inbred lines and then selected a core collection of lines for more detailed analysis with an emphasis on traits relevant to the use of grasses as biofuel and grain crops.
24423101	4	40	dep	RESULTS	487:493	arg1	surveyed					498:505	surveyed	498:505	surveyed the phenotypic diversity in a large collection of inbred lines	498:568	RESULTS We surveyed the phenotypic diversity in a large collection of inbred lines and then selected a core collection of lines for more detailed analysis with an emphasis on traits relevant to the use of grasses as biofuel and grain crops.
24423101	8	41	theme	variation	1353:1361	arg1	basis					1320:1324	the genetic basis	1308:1324	the genetic basis of the observed phenotypic variation	1308:1361	CONCLUSION These characterizations can provide the criteria for selecting diverse lines for future investigations of the genetic basis of the observed phenotypic variation.
24423101	7	42	theme	ferulic	1117:1123	arg1	acid					1125:1128	ferulic acid	1117:1128	ferulic acid bound to hemicellulose	1117:1151	In all cases, we observed extensive natural variation including a two-fold variation in stem density, four-fold variation in ferulic acid bound to hemicellulose, and 1.7-fold variation in seed mass.
24423101	1	43	theme	biology	210:216	arg1	aspects					193:199	various aspects	185:199	various aspects of grass biology	185:216	BACKGROUND The model grass Brachypodium distachyon is increasingly used to study various aspects of grass biology.
24423101	4	44	theme	grasses	692:698	arg1	use					685:687	the use	681:687	the use of grasses as biofuel and grain crops	681:725	RESULTS We surveyed the phenotypic diversity in a large collection of inbred lines and then selected a core collection of lines for more detailed analysis with an emphasis on traits relevant to the use of grasses as biofuel and grain crops.
24423101	2	45	theme	diverse	245:251	arg1	collection					253:262	A large and genotypically diverse collection	219:262	A large and genotypically diverse collection of B. distachyon germplasm	219:289	A large and genotypically diverse collection of B. distachyon germplasm has been assembled by the research community.
24423101	7	46	theme	four-fold	1094:1102	arg1	variation					1104:1112	four-fold variation	1094:1112	four-fold variation in ferulic acid bound to hemicellulose	1094:1151	In all cases, we observed extensive natural variation including a two-fold variation in stem density, four-fold variation in ferulic acid bound to hemicellulose, and 1.7-fold variation in seed mass.
24423101	3	47	theme	many	424:427	arg1	areas					429:433	many areas	424:433	many areas	424:433	The natural variation in this collection can serve as a powerful experimental tool for many areas of inquiry, including investigating biomass traits.
24423101	3	47	theme	many	424:427	arg1	traits					479:484	investigating biomass traits	457:484	investigating biomass traits	457:484	The natural variation in this collection can serve as a powerful experimental tool for many areas of inquiry, including investigating biomass traits.
24423101	4	48	theme	large	537:541	arg1	collection					543:552	a large collection	535:552	a large collection of inbred lines	535:568	RESULTS We surveyed the phenotypic diversity in a large collection of inbred lines and then selected a core collection of lines for more detailed analysis with an emphasis on traits relevant to the use of grasses as biofuel and grain crops.
24423101	7	49	theme	extensive	1018:1026	arg1	variation					1036:1044	extensive natural variation	1018:1044	extensive natural variation including a two-fold variation in stem density, four-fold variation in ferulic acid bound to hemicellulose, and 1.7-fold variation in seed mass	1018:1188	In all cases, we observed extensive natural variation including a two-fold variation in stem density, four-fold variation in ferulic acid bound to hemicellulose, and 1.7-fold variation in seed mass.
24423101	3	50	theme	investigating	457:469	arg1	traits					479:484	investigating biomass traits	457:484	investigating biomass traits	457:484	The natural variation in this collection can serve as a powerful experimental tool for many areas of inquiry, including investigating biomass traits.
24423101	0	51	theme	collection	52:61	arg1	Selection					0:8	Selection	0:8	Selection	0:8	Selection and phenotypic characterization of a core collection of Brachypodium distachyon inbred lines.
24423101	0	51	theme	collection	52:61	arg1	characterization					25:40	phenotypic characterization	14:40	phenotypic characterization	14:40	Selection and phenotypic characterization of a core collection of Brachypodium distachyon inbred lines.
24423101	4	52	theme	inbred	557:562	arg1	lines					564:568	inbred lines	557:568	inbred lines	557:568	RESULTS We surveyed the phenotypic diversity in a large collection of inbred lines and then selected a core collection of lines for more detailed analysis with an emphasis on traits relevant to the use of grasses as biofuel and grain crops.
24423101	8	53	theme	observed	1333:1340	arg1	variation					1353:1361	the observed phenotypic variation	1329:1361	the observed phenotypic variation	1329:1361	CONCLUSION These characterizations can provide the criteria for selecting diverse lines for future investigations of the genetic basis of the observed phenotypic variation.
24423101	1	54	theme	model	119:123	arg1	distachyon					144:153	The model grass Brachypodium distachyon	115:153	The model grass Brachypodium distachyon	115:153	BACKGROUND The model grass Brachypodium distachyon is increasingly used to study various aspects of grass biology.
24423101	0	55	theme	core	47:50	arg1	collection					52:61	a core collection	45:61	a core collection of Brachypodium distachyon inbred lines	45:101	Selection and phenotypic characterization of a core collection of Brachypodium distachyon inbred lines.
24423101	6	56	theme	infrared	908:915	arg1	spectroscopy					917:928	near infrared spectroscopy	903:928	near infrared spectroscopy (NIR)	903:934	We also surveyed differences in cell wall composition using near infrared spectroscopy (NIR) and comprehensive microarray polymer profiling (CoMPP).
24423101	6	56	theme	infrared	908:915	arg1	NIR					931:933	NIR	931:933	NIR	931:933	We also surveyed differences in cell wall composition using near infrared spectroscopy (NIR) and comprehensive microarray polymer profiling (CoMPP).
24423101	2	57	theme	large	221:225	arg1	collection					253:262	A large and genotypically diverse collection	219:262	A large and genotypically diverse collection of B. distachyon germplasm	219:289	A large and genotypically diverse collection of B. distachyon germplasm has been assembled by the research community.
24423101	1	58	theme	grass	125:129	arg1	distachyon					144:153	The model grass Brachypodium distachyon	115:153	The model grass Brachypodium distachyon	115:153	BACKGROUND The model grass Brachypodium distachyon is increasingly used to study various aspects of grass biology.
24423101	6	59	theme	near	903:906	arg1	spectroscopy					917:928	near infrared spectroscopy	903:928	near infrared spectroscopy (NIR)	903:934	We also surveyed differences in cell wall composition using near infrared spectroscopy (NIR) and comprehensive microarray polymer profiling (CoMPP).
24423101	6	59	theme	near	903:906	arg1	NIR					931:933	NIR	931:933	NIR	931:933	We also surveyed differences in cell wall composition using near infrared spectroscopy (NIR) and comprehensive microarray polymer profiling (CoMPP).
24423101	2	60	theme	distachyon	270:279	arg1	germplasm					281:289	B. distachyon germplasm	267:289	B. distachyon germplasm	267:289	A large and genotypically diverse collection of B. distachyon germplasm has been assembled by the research community.
24423101	7	61	from	variation	1104:1112	arg1	mass					1185:1188	seed mass	1180:1188	seed mass	1180:1188	In all cases, we observed extensive natural variation including a two-fold variation in stem density, four-fold variation in ferulic acid bound to hemicellulose, and 1.7-fold variation in seed mass.
24423101	7	61	from	variation	1104:1112	arg1	density					1085:1091	stem density	1080:1091	stem density	1080:1091	In all cases, we observed extensive natural variation including a two-fold variation in stem density, four-fold variation in ferulic acid bound to hemicellulose, and 1.7-fold variation in seed mass.
24423101	7	61	from	variation	1104:1112	arg1	acid					1125:1128	ferulic acid	1117:1128	ferulic acid bound to hemicellulose	1117:1151	In all cases, we observed extensive natural variation including a two-fold variation in stem density, four-fold variation in ferulic acid bound to hemicellulose, and 1.7-fold variation in seed mass.
24423101	1	62	theme	Brachypodium	131:142	arg1	distachyon					144:153	The model grass Brachypodium distachyon	115:153	The model grass Brachypodium distachyon	115:153	BACKGROUND The model grass Brachypodium distachyon is increasingly used to study various aspects of grass biology.
24423101	4	63	theme	lines	609:613	arg1	collection					595:604	a core collection	588:604	a core collection of lines	588:613	RESULTS We surveyed the phenotypic diversity in a large collection of inbred lines and then selected a core collection of lines for more detailed analysis with an emphasis on traits relevant to the use of grasses as biofuel and grain crops.
24423101	4	64	with	analysis	633:640	arg1	emphasis					650:657	an emphasis	647:657	an emphasis on traits relevant to the use of grasses as biofuel and grain crops	647:725	RESULTS We surveyed the phenotypic diversity in a large collection of inbred lines and then selected a core collection of lines for more detailed analysis with an emphasis on traits relevant to the use of grasses as biofuel and grain crops.
24423101	2	65	theme	B.	267:268	arg1	germplasm					281:289	B. distachyon germplasm	267:289	B. distachyon germplasm	267:289	A large and genotypically diverse collection of B. distachyon germplasm has been assembled by the research community.
24423101	7	66	from	variation	1067:1075	arg1	mass					1185:1188	seed mass	1180:1188	seed mass	1180:1188	In all cases, we observed extensive natural variation including a two-fold variation in stem density, four-fold variation in ferulic acid bound to hemicellulose, and 1.7-fold variation in seed mass.
24423101	7	66	from	variation	1067:1075	arg1	density					1085:1091	stem density	1080:1091	stem density	1080:1091	In all cases, we observed extensive natural variation including a two-fold variation in stem density, four-fold variation in ferulic acid bound to hemicellulose, and 1.7-fold variation in seed mass.
24423101	7	66	from	variation	1067:1075	arg1	acid					1125:1128	ferulic acid	1117:1128	ferulic acid bound to hemicellulose	1117:1151	In all cases, we observed extensive natural variation including a two-fold variation in stem density, four-fold variation in ferulic acid bound to hemicellulose, and 1.7-fold variation in seed mass.
24423101	8	67	theme	genetic	1312:1318	arg1	basis					1320:1324	the genetic basis	1308:1324	the genetic basis of the observed phenotypic variation	1308:1361	CONCLUSION These characterizations can provide the criteria for selecting diverse lines for future investigations of the genetic basis of the observed phenotypic variation.
24423101	0	68	theme	Brachypodium	66:77	arg1	lines					97:101	Brachypodium distachyon inbred lines	66:101	Brachypodium distachyon inbred lines	66:101	Selection and phenotypic characterization of a core collection of Brachypodium distachyon inbred lines.
24423101	3	69	from	variation	349:357	arg1	collection					367:376	this collection	362:376	this collection	362:376	The natural variation in this collection can serve as a powerful experimental tool for many areas of inquiry, including investigating biomass traits.
24423101	1	70	theme	grass	204:208	arg1	biology					210:216	grass biology	204:216	grass biology	204:216	BACKGROUND The model grass Brachypodium distachyon is increasingly used to study various aspects of grass biology.
24423101	1	71	dep	BACKGROUND	104:113	arg1	used					171:174	used	171:174	is increasingly used to study various aspects of grass biology	155:216	BACKGROUND The model grass Brachypodium distachyon is increasingly used to study various aspects of grass biology.
24423101	3	72	theme	experimental	402:413	arg1	tool					415:418	a powerful experimental tool	391:418	a powerful experimental tool for many areas of inquiry, including investigating biomass traits	391:484	The natural variation in this collection can serve as a powerful experimental tool for many areas of inquiry, including investigating biomass traits.
24423101	3	72	theme	experimental	402:413	arg1	variation					349:357	The natural variation	337:357	The natural variation in this collection	337:376	The natural variation in this collection can serve as a powerful experimental tool for many areas of inquiry, including investigating biomass traits.
24423101	4	73	theme	phenotypic	511:520	arg1	diversity					522:530	the phenotypic diversity	507:530	the phenotypic diversity in a large collection of inbred lines	507:568	RESULTS We surveyed the phenotypic diversity in a large collection of inbred lines and then selected a core collection of lines for more detailed analysis with an emphasis on traits relevant to the use of grasses as biofuel and grain crops.
24423101	6	74	theme	comprehensive	940:952	arg1	profiling					973:981	comprehensive microarray polymer profiling	940:981	comprehensive microarray polymer profiling (CoMPP)	940:989	We also surveyed differences in cell wall composition using near infrared spectroscopy (NIR) and comprehensive microarray polymer profiling (CoMPP).
24423101	6	74	theme	comprehensive	940:952	arg1	CoMPP					984:988	CoMPP	984:988	CoMPP	984:988	We also surveyed differences in cell wall composition using near infrared spectroscopy (NIR) and comprehensive microarray polymer profiling (CoMPP).
24423101	8	75	theme	future	1283:1288	arg1	investigations					1290:1303	future investigations	1283:1303	future investigations of the genetic basis of the observed phenotypic variation	1283:1361	CONCLUSION These characterizations can provide the criteria for selecting diverse lines for future investigations of the genetic basis of the observed phenotypic variation.
27693726	3	0	theme	rhodophyta	674:683	arg1	growth					664:669	the growth	660:669	the growth of rhodophyta	660:683	The results demonstrate that green light plays a significant role in the growth of rhodophyta, due to phycobiliproteins being able to harvest green wavelengths where chlorophyll pigments absorb poorly.
27693726	2	1	theme	interest	581:588	arg1	compounds					557:565	multiple compounds	548:565	multiple compounds of commercial interest	548:588	Phycobiliprotein, fatty acids, exopolysaccharides, pigment content, and the main macromolecules composition were analysed to determine the effect of wavelength on multiple compounds of commercial interest.
27693726	1	2	dep	combination	256:266	arg1	red					288:290	red	288:290	red	288:290	The effect of specific narrow light-emitting diode (LED) wavelengths (red, green, blue) and a combination of LED wavelengths (red, green and blue - RGB) on biomass composition produced by Porphyridium purpureum is studied.
27693726	1	2	dep	combination	256:266	arg1	RGB					310:312	RGB	310:312	RGB	310:312	The effect of specific narrow light-emitting diode (LED) wavelengths (red, green, blue) and a combination of LED wavelengths (red, green and blue - RGB) on biomass composition produced by Porphyridium purpureum is studied.
27693726	1	2	dep	combination	256:266	arg1	blue					303:306	blue	303:306	blue	303:306	The effect of specific narrow light-emitting diode (LED) wavelengths (red, green, blue) and a combination of LED wavelengths (red, green and blue - RGB) on biomass composition produced by Porphyridium purpureum is studied.
27693726	1	2	dep	combination	256:266	arg1	green					293:297	green	293:297	green	293:297	The effect of specific narrow light-emitting diode (LED) wavelengths (red, green, blue) and a combination of LED wavelengths (red, green and blue - RGB) on biomass composition produced by Porphyridium purpureum is studied.
27693726	2	3	theme	commercial	570:579	arg1	interest					581:588	commercial interest	570:588	commercial interest	570:588	Phycobiliprotein, fatty acids, exopolysaccharides, pigment content, and the main macromolecules composition were analysed to determine the effect of wavelength on multiple compounds of commercial interest.
27693726	1	4	theme	specific	176:183	arg1	diode					207:211	specific narrow light-emitting diode	176:211	specific narrow light-emitting diode (LED) wavelengths (red, green, blue)	176:248	The effect of specific narrow light-emitting diode (LED) wavelengths (red, green, blue) and a combination of LED wavelengths (red, green and blue - RGB) on biomass composition produced by Porphyridium purpureum is studied.
27693726	5	5	theme	photo-adaption	1140:1153	arg1	accumulation					1087:1098	This increased accumulation	1072:1098	This increased accumulation	1072:1098	This increased accumulation is likely to be the combination of both photo-adaption and photo-protection, under the combined specific wavelengths employed.
27693726	5	5	theme	photo-adaption	1140:1153	arg1	combination					1120:1130	the combination	1116:1130	the combination of both photo-adaption and photo-protection	1116:1174	This increased accumulation is likely to be the combination of both photo-adaption and photo-protection, under the combined specific wavelengths employed.
27693726	0	6	theme	multiple	105:112	arg1	compounds					125:133	multiple high-value compounds	105:133	multiple high-value compounds	105:133	Utilising light-emitting diodes of specific narrow wavelengths for the optimization and co-production of multiple high-value compounds in Porphyridium purpureum.
27693726	5	7	theme	increased	1077:1085	arg1	accumulation					1087:1098	This increased accumulation	1072:1098	This increased accumulation	1072:1098	This increased accumulation is likely to be the combination of both photo-adaption and photo-protection, under the combined specific wavelengths employed.
27693726	5	7	theme	increased	1077:1085	arg1	combination					1120:1130	the combination	1116:1130	the combination of both photo-adaption and photo-protection	1116:1174	This increased accumulation is likely to be the combination of both photo-adaption and photo-protection, under the combined specific wavelengths employed.
27693726	5	8	theme	photo-protection	1159:1174	arg1	accumulation					1087:1098	This increased accumulation	1072:1098	This increased accumulation	1072:1098	This increased accumulation is likely to be the combination of both photo-adaption and photo-protection, under the combined specific wavelengths employed.
27693726	5	8	theme	photo-protection	1159:1174	arg1	combination					1120:1130	the combination	1116:1130	the combination of both photo-adaption and photo-protection	1116:1174	This increased accumulation is likely to be the combination of both photo-adaption and photo-protection, under the combined specific wavelengths employed.
27693726	0	9	theme	compounds	125:133	arg1	co-production					88:100	co-production	88:100	co-production	88:100	Utilising light-emitting diodes of specific narrow wavelengths for the optimization and co-production of multiple high-value compounds in Porphyridium purpureum.
27693726	0	9	theme	compounds	125:133	arg1	optimization					71:82	optimization	71:82	optimization	71:82	Utilising light-emitting diodes of specific narrow wavelengths for the optimization and co-production of multiple high-value compounds in Porphyridium purpureum.
27693726	1	10	from	effect	166:171	arg1	composition					326:336	biomass composition	318:336	biomass composition produced by Porphyridium purpureum	318:371	The effect of specific narrow light-emitting diode (LED) wavelengths (red, green, blue) and a combination of LED wavelengths (red, green and blue - RGB) on biomass composition produced by Porphyridium purpureum is studied.
27693726	1	11	theme	narrow	185:190	arg1	diode					207:211	specific narrow light-emitting diode	176:211	specific narrow light-emitting diode (LED) wavelengths (red, green, blue)	176:248	The effect of specific narrow light-emitting diode (LED) wavelengths (red, green, blue) and a combination of LED wavelengths (red, green and blue - RGB) on biomass composition produced by Porphyridium purpureum is studied.
27693726	0	12	theme	high-value	114:123	arg1	compounds					125:133	multiple high-value compounds	105:133	multiple high-value compounds	105:133	Utilising light-emitting diodes of specific narrow wavelengths for the optimization and co-production of multiple high-value compounds in Porphyridium purpureum.
27693726	1	13	theme	biomass	318:324	arg1	composition					326:336	biomass composition	318:336	biomass composition produced by Porphyridium purpureum	318:371	The effect of specific narrow light-emitting diode (LED) wavelengths (red, green, blue) and a combination of LED wavelengths (red, green and blue - RGB) on biomass composition produced by Porphyridium purpureum is studied.
27693726	4	14	theme	LED	824:826	arg1	wavelengths					828:838	multi-chromatic LED wavelengths	808:838	multi-chromatic LED wavelengths	808:838	However, under multi-chromatic LED wavelengths, P. purpureum biomass accumulated the highest yield of valuable products such as eicosapentaenoic acid (∼2.9% DW), zeaxanthin (∼586μgg-1DW), β-carotene (397μgg-1DW), exopolysaccharides (2.05g/L-1), and phycobiliproteins (∼4.8% DW).
27693726	1	15	theme	light-emitting	192:205	arg1	diode					207:211	specific narrow light-emitting diode	176:211	specific narrow light-emitting diode (LED) wavelengths (red, green, blue)	176:248	The effect of specific narrow light-emitting diode (LED) wavelengths (red, green, blue) and a combination of LED wavelengths (red, green and blue - RGB) on biomass composition produced by Porphyridium purpureum is studied.
27693726	0	16	theme	light-emitting	10:23	arg1	diodes					25:30	light-emitting diodes	10:30	light-emitting diodes of specific narrow wavelengths for the optimization and co-production of multiple high-value compounds in Porphyridium purpureum	10:159	Utilising light-emitting diodes of specific narrow wavelengths for the optimization and co-production of multiple high-value compounds in Porphyridium purpureum.
27693726	4	17	theme	P.	841:842	arg1	biomass					854:860	P. purpureum biomass	841:860	P. purpureum biomass	841:860	However, under multi-chromatic LED wavelengths, P. purpureum biomass accumulated the highest yield of valuable products such as eicosapentaenoic acid (∼2.9% DW), zeaxanthin (∼586μgg-1DW), β-carotene (397μgg-1DW), exopolysaccharides (2.05g/L-1), and phycobiliproteins (∼4.8% DW).
27693726	1	18	theme	diode	207:211	arg1	wavelengths					219:229	specific narrow light-emitting diode (LED) wavelengths	176:229	specific narrow light-emitting diode (LED) wavelengths (red, green, blue)	176:248	The effect of specific narrow light-emitting diode (LED) wavelengths (red, green, blue) and a combination of LED wavelengths (red, green and blue - RGB) on biomass composition produced by Porphyridium purpureum is studied.
27693726	1	19	theme	combination	256:266	arg1	effect					166:171	The effect	162:171	The effect of specific narrow light-emitting diode (LED) wavelengths (red, green, blue) and a combination of LED wavelengths (red, green and blue - RGB) on biomass composition produced by Porphyridium purpureum	162:371	The effect of specific narrow light-emitting diode (LED) wavelengths (red, green, blue) and a combination of LED wavelengths (red, green and blue - RGB) on biomass composition produced by Porphyridium purpureum is studied.
27693726	4	20	theme	purpureum	844:852	arg1	biomass					854:860	P. purpureum biomass	841:860	P. purpureum biomass	841:860	However, under multi-chromatic LED wavelengths, P. purpureum biomass accumulated the highest yield of valuable products such as eicosapentaenoic acid (∼2.9% DW), zeaxanthin (∼586μgg-1DW), β-carotene (397μgg-1DW), exopolysaccharides (2.05g/L-1), and phycobiliproteins (∼4.8% DW).
27693726	2	21	theme	fatty	403:407	arg1	acids					409:413	fatty acids	403:413	fatty acids	403:413	Phycobiliprotein, fatty acids, exopolysaccharides, pigment content, and the main macromolecules composition were analysed to determine the effect of wavelength on multiple compounds of commercial interest.
27693726	3	22	theme	harvest	725:731	arg1	wavelengths					739:749	harvest green wavelengths	725:749	harvest green wavelengths where chlorophyll pigments absorb poorly	725:790	The results demonstrate that green light plays a significant role in the growth of rhodophyta, due to phycobiliproteins being able to harvest green wavelengths where chlorophyll pigments absorb poorly.
27693726	0	23	dep	optimization	71:82	arg1	the					67:69	the	67:69	the	67:69	Utilising light-emitting diodes of specific narrow wavelengths for the optimization and co-production of multiple high-value compounds in Porphyridium purpureum.
27693726	1	24	theme	LED	271:273	arg1	wavelengths					275:285	LED wavelengths	271:285	LED wavelengths	271:285	The effect of specific narrow light-emitting diode (LED) wavelengths (red, green, blue) and a combination of LED wavelengths (red, green and blue - RGB) on biomass composition produced by Porphyridium purpureum is studied.
27693726	4	25	theme	products	904:911	arg1	yield					886:890	the highest yield	874:890	the highest yield of valuable products such as eicosapentaenoic acid (∼2.9% DW), zeaxanthin (∼586μgg-1DW), β-carotene (397μgg-1DW), exopolysaccharides (2.05g/L-1), and phycobiliproteins (∼4.8% DW)	874:1069	However, under multi-chromatic LED wavelengths, P. purpureum biomass accumulated the highest yield of valuable products such as eicosapentaenoic acid (∼2.9% DW), zeaxanthin (∼586μgg-1DW), β-carotene (397μgg-1DW), exopolysaccharides (2.05g/L-1), and phycobiliproteins (∼4.8% DW).
27693726	0	26	from	co-production	88:100	arg1	purpureum					151:159	Porphyridium purpureum	138:159	Porphyridium purpureum	138:159	Utilising light-emitting diodes of specific narrow wavelengths for the optimization and co-production of multiple high-value compounds in Porphyridium purpureum.
27693726	0	27	theme	narrow	44:49	arg1	wavelengths					51:61	specific narrow wavelengths	35:61	specific narrow wavelengths	35:61	Utilising light-emitting diodes of specific narrow wavelengths for the optimization and co-production of multiple high-value compounds in Porphyridium purpureum.
27693726	1	28	theme	wavelengths	275:285	arg1	combination					256:266	a combination	254:266	a combination of LED wavelengths (red, green and blue - RGB)	254:313	The effect of specific narrow light-emitting diode (LED) wavelengths (red, green, blue) and a combination of LED wavelengths (red, green and blue - RGB) on biomass composition produced by Porphyridium purpureum is studied.
27693726	1	28	theme	wavelengths	275:285	arg1	wavelengths					219:229	specific narrow light-emitting diode (LED) wavelengths	176:229	specific narrow light-emitting diode (LED) wavelengths (red, green, blue)	176:248	The effect of specific narrow light-emitting diode (LED) wavelengths (red, green, blue) and a combination of LED wavelengths (red, green and blue - RGB) on biomass composition produced by Porphyridium purpureum is studied.
27693726	4	29	theme	eicosapentaenoic	921:936	arg1	acid					938:941	eicosapentaenoic acid	921:941	eicosapentaenoic acid (∼2.9% DW)	921:952	However, under multi-chromatic LED wavelengths, P. purpureum biomass accumulated the highest yield of valuable products such as eicosapentaenoic acid (∼2.9% DW), zeaxanthin (∼586μgg-1DW), β-carotene (397μgg-1DW), exopolysaccharides (2.05g/L-1), and phycobiliproteins (∼4.8% DW).
27693726	4	29	theme	eicosapentaenoic	921:936	arg1	DW					950:951	∼2.9% DW	944:951	∼2.9% DW	944:951	However, under multi-chromatic LED wavelengths, P. purpureum biomass accumulated the highest yield of valuable products such as eicosapentaenoic acid (∼2.9% DW), zeaxanthin (∼586μgg-1DW), β-carotene (397μgg-1DW), exopolysaccharides (2.05g/L-1), and phycobiliproteins (∼4.8% DW).
27693726	1	30	dep	diode	207:211	arg1	LED					214:216	LED	214:216	LED	214:216	The effect of specific narrow light-emitting diode (LED) wavelengths (red, green, blue) and a combination of LED wavelengths (red, green and blue - RGB) on biomass composition produced by Porphyridium purpureum is studied.
27693726	4	31	theme	%	1065:1065	arg1	DW					1067:1068	∼4.8% DW	1061:1068	∼4.8% DW	1061:1068	However, under multi-chromatic LED wavelengths, P. purpureum biomass accumulated the highest yield of valuable products such as eicosapentaenoic acid (∼2.9% DW), zeaxanthin (∼586μgg-1DW), β-carotene (397μgg-1DW), exopolysaccharides (2.05g/L-1), and phycobiliproteins (∼4.8% DW).
27693726	4	31	theme	%	1065:1065	arg1	phycobiliproteins					1042:1058	phycobiliproteins	1042:1058	phycobiliproteins (∼4.8% DW)	1042:1069	However, under multi-chromatic LED wavelengths, P. purpureum biomass accumulated the highest yield of valuable products such as eicosapentaenoic acid (∼2.9% DW), zeaxanthin (∼586μgg-1DW), β-carotene (397μgg-1DW), exopolysaccharides (2.05g/L-1), and phycobiliproteins (∼4.8% DW).
27693726	1	32	theme	wavelengths	219:229	arg1	effect					166:171	The effect	162:171	The effect of specific narrow light-emitting diode (LED) wavelengths (red, green, blue) and a combination of LED wavelengths (red, green and blue - RGB) on biomass composition produced by Porphyridium purpureum	162:371	The effect of specific narrow light-emitting diode (LED) wavelengths (red, green, blue) and a combination of LED wavelengths (red, green and blue - RGB) on biomass composition produced by Porphyridium purpureum is studied.
27693726	0	33	theme	specific	35:42	arg1	wavelengths					51:61	specific narrow wavelengths	35:61	specific narrow wavelengths	35:61	Utilising light-emitting diodes of specific narrow wavelengths for the optimization and co-production of multiple high-value compounds in Porphyridium purpureum.
27693726	2	34	theme	pigment	436:442	arg1	content					444:450	pigment content	436:450	pigment content	436:450	Phycobiliprotein, fatty acids, exopolysaccharides, pigment content, and the main macromolecules composition were analysed to determine the effect of wavelength on multiple compounds of commercial interest.
27693726	0	35	from	optimization	71:82	arg1	purpureum					151:159	Porphyridium purpureum	138:159	Porphyridium purpureum	138:159	Utilising light-emitting diodes of specific narrow wavelengths for the optimization and co-production of multiple high-value compounds in Porphyridium purpureum.
27693726	2	36	theme	macromolecules	466:479	arg1	composition					481:491	the main macromolecules composition	457:491	the main macromolecules composition	457:491	Phycobiliprotein, fatty acids, exopolysaccharides, pigment content, and the main macromolecules composition were analysed to determine the effect of wavelength on multiple compounds of commercial interest.
27693726	3	37	theme	chlorophyll	757:767	arg1	pigments					769:776	chlorophyll pigments	757:776	chlorophyll pigments	757:776	The results demonstrate that green light plays a significant role in the growth of rhodophyta, due to phycobiliproteins being able to harvest green wavelengths where chlorophyll pigments absorb poorly.
27693726	2	38	theme	multiple	548:555	arg1	compounds					557:565	multiple compounds	548:565	multiple compounds of commercial interest	548:588	Phycobiliprotein, fatty acids, exopolysaccharides, pigment content, and the main macromolecules composition were analysed to determine the effect of wavelength on multiple compounds of commercial interest.
27693726	3	39	theme	green	620:624	arg1	light					626:630	green light	620:630	green light	620:630	The results demonstrate that green light plays a significant role in the growth of rhodophyta, due to phycobiliproteins being able to harvest green wavelengths where chlorophyll pigments absorb poorly.
27693726	4	40	theme	multi-chromatic	808:822	arg1	wavelengths					828:838	multi-chromatic LED wavelengths	808:838	multi-chromatic LED wavelengths	808:838	However, under multi-chromatic LED wavelengths, P. purpureum biomass accumulated the highest yield of valuable products such as eicosapentaenoic acid (∼2.9% DW), zeaxanthin (∼586μgg-1DW), β-carotene (397μgg-1DW), exopolysaccharides (2.05g/L-1), and phycobiliproteins (∼4.8% DW).
27693726	4	41	theme	valuable	895:902	arg1	exopolysaccharides					1006:1023	exopolysaccharides	1006:1023	exopolysaccharides (2.05g/L-1)	1006:1035	However, under multi-chromatic LED wavelengths, P. purpureum biomass accumulated the highest yield of valuable products such as eicosapentaenoic acid (∼2.9% DW), zeaxanthin (∼586μgg-1DW), β-carotene (397μgg-1DW), exopolysaccharides (2.05g/L-1), and phycobiliproteins (∼4.8% DW).
27693726	4	41	theme	valuable	895:902	arg1	β-carotene					981:990	β-carotene	981:990	β-carotene (397μgg-1DW)	981:1003	However, under multi-chromatic LED wavelengths, P. purpureum biomass accumulated the highest yield of valuable products such as eicosapentaenoic acid (∼2.9% DW), zeaxanthin (∼586μgg-1DW), β-carotene (397μgg-1DW), exopolysaccharides (2.05g/L-1), and phycobiliproteins (∼4.8% DW).
27693726	4	41	theme	valuable	895:902	arg1	zeaxanthin					955:964	zeaxanthin	955:964	zeaxanthin (∼586μgg-1DW)	955:978	However, under multi-chromatic LED wavelengths, P. purpureum biomass accumulated the highest yield of valuable products such as eicosapentaenoic acid (∼2.9% DW), zeaxanthin (∼586μgg-1DW), β-carotene (397μgg-1DW), exopolysaccharides (2.05g/L-1), and phycobiliproteins (∼4.8% DW).
27693726	4	41	theme	valuable	895:902	arg1	acid					938:941	eicosapentaenoic acid	921:941	eicosapentaenoic acid (∼2.9% DW)	921:952	However, under multi-chromatic LED wavelengths, P. purpureum biomass accumulated the highest yield of valuable products such as eicosapentaenoic acid (∼2.9% DW), zeaxanthin (∼586μgg-1DW), β-carotene (397μgg-1DW), exopolysaccharides (2.05g/L-1), and phycobiliproteins (∼4.8% DW).
27693726	4	41	theme	valuable	895:902	arg1	products					904:911	valuable products	895:911	valuable products such as eicosapentaenoic acid (∼2.9% DW), zeaxanthin (∼586μgg-1DW), β-carotene (397μgg-1DW), exopolysaccharides (2.05g/L-1), and phycobiliproteins (∼4.8% DW)	895:1069	However, under multi-chromatic LED wavelengths, P. purpureum biomass accumulated the highest yield of valuable products such as eicosapentaenoic acid (∼2.9% DW), zeaxanthin (∼586μgg-1DW), β-carotene (397μgg-1DW), exopolysaccharides (2.05g/L-1), and phycobiliproteins (∼4.8% DW).
27693726	4	41	theme	valuable	895:902	arg1	phycobiliproteins					1042:1058	phycobiliproteins	1042:1058	phycobiliproteins (∼4.8% DW)	1042:1069	However, under multi-chromatic LED wavelengths, P. purpureum biomass accumulated the highest yield of valuable products such as eicosapentaenoic acid (∼2.9% DW), zeaxanthin (∼586μgg-1DW), β-carotene (397μgg-1DW), exopolysaccharides (2.05g/L-1), and phycobiliproteins (∼4.8% DW).
27693726	0	42	theme	wavelengths	51:61	arg1	diodes					25:30	light-emitting diodes	10:30	light-emitting diodes of specific narrow wavelengths for the optimization and co-production of multiple high-value compounds in Porphyridium purpureum	10:159	Utilising light-emitting diodes of specific narrow wavelengths for the optimization and co-production of multiple high-value compounds in Porphyridium purpureum.
27693726	3	43	theme	green	733:737	arg1	wavelengths					739:749	harvest green wavelengths	725:749	harvest green wavelengths where chlorophyll pigments absorb poorly	725:790	The results demonstrate that green light plays a significant role in the growth of rhodophyta, due to phycobiliproteins being able to harvest green wavelengths where chlorophyll pigments absorb poorly.
27693726	4	44	theme	highest	878:884	arg1	yield					886:890	the highest yield	874:890	the highest yield of valuable products such as eicosapentaenoic acid (∼2.9% DW), zeaxanthin (∼586μgg-1DW), β-carotene (397μgg-1DW), exopolysaccharides (2.05g/L-1), and phycobiliproteins (∼4.8% DW)	874:1069	However, under multi-chromatic LED wavelengths, P. purpureum biomass accumulated the highest yield of valuable products such as eicosapentaenoic acid (∼2.9% DW), zeaxanthin (∼586μgg-1DW), β-carotene (397μgg-1DW), exopolysaccharides (2.05g/L-1), and phycobiliproteins (∼4.8% DW).
27693726	1	45	dep	red	232:234	arg1	green					237:241	green	237:241	green	237:241	The effect of specific narrow light-emitting diode (LED) wavelengths (red, green, blue) and a combination of LED wavelengths (red, green and blue - RGB) on biomass composition produced by Porphyridium purpureum is studied.
27693726	1	45	dep	red	232:234	arg1	blue					244:247	blue	244:247	blue	244:247	The effect of specific narrow light-emitting diode (LED) wavelengths (red, green, blue) and a combination of LED wavelengths (red, green and blue - RGB) on biomass composition produced by Porphyridium purpureum is studied.
27693726	4	46	theme	%	948:948	arg1	acid					938:941	eicosapentaenoic acid	921:941	eicosapentaenoic acid (∼2.9% DW)	921:952	However, under multi-chromatic LED wavelengths, P. purpureum biomass accumulated the highest yield of valuable products such as eicosapentaenoic acid (∼2.9% DW), zeaxanthin (∼586μgg-1DW), β-carotene (397μgg-1DW), exopolysaccharides (2.05g/L-1), and phycobiliproteins (∼4.8% DW).
27693726	4	46	theme	%	948:948	arg1	DW					950:951	∼2.9% DW	944:951	∼2.9% DW	944:951	However, under multi-chromatic LED wavelengths, P. purpureum biomass accumulated the highest yield of valuable products such as eicosapentaenoic acid (∼2.9% DW), zeaxanthin (∼586μgg-1DW), β-carotene (397μgg-1DW), exopolysaccharides (2.05g/L-1), and phycobiliproteins (∼4.8% DW).
27693726	2	47	theme	wavelength	534:543	arg1	effect					524:529	the effect	520:529	the effect of wavelength on multiple compounds of commercial interest	520:588	Phycobiliprotein, fatty acids, exopolysaccharides, pigment content, and the main macromolecules composition were analysed to determine the effect of wavelength on multiple compounds of commercial interest.
27693726	1	48	dep	wavelengths	219:229	arg1	red					232:234	red	232:234	red	232:234	The effect of specific narrow light-emitting diode (LED) wavelengths (red, green, blue) and a combination of LED wavelengths (red, green and blue - RGB) on biomass composition produced by Porphyridium purpureum is studied.
27693726	2	49	theme	main	461:464	arg1	composition					481:491	the main macromolecules composition	457:491	the main macromolecules composition	457:491	Phycobiliprotein, fatty acids, exopolysaccharides, pigment content, and the main macromolecules composition were analysed to determine the effect of wavelength on multiple compounds of commercial interest.
27693726	3	50	theme	significant	640:650	arg1	role					652:655	a significant role	638:655	a significant role	638:655	The results demonstrate that green light plays a significant role in the growth of rhodophyta, due to phycobiliproteins being able to harvest green wavelengths where chlorophyll pigments absorb poorly.
27693726	2	51	from	effect	524:529	arg1	compounds					557:565	multiple compounds	548:565	multiple compounds of commercial interest	548:588	Phycobiliprotein, fatty acids, exopolysaccharides, pigment content, and the main macromolecules composition were analysed to determine the effect of wavelength on multiple compounds of commercial interest.
27693726	5	52	theme	specific	1196:1203	arg1	wavelengths					1205:1215	the combined specific wavelengths	1183:1215	the combined specific wavelengths employed	1183:1224	This increased accumulation is likely to be the combination of both photo-adaption and photo-protection, under the combined specific wavelengths employed.
27693726	5	53	theme	combined	1187:1194	arg1	wavelengths					1205:1215	the combined specific wavelengths	1183:1215	the combined specific wavelengths employed	1183:1224	This increased accumulation is likely to be the combination of both photo-adaption and photo-protection, under the combined specific wavelengths employed.
26716240	5	0	theme	viscometric	766:776	arg1	function					812:819	a function	810:819	a function of the number of passages of refined/bleached suspensions through a defibrillator	810:901	Morphological, structural, thermal, optical and viscometric properties were investigated as a function of the number of passages of refined/bleached suspensions through a defibrillator.
26716240	5	0	theme	viscometric	766:776	arg1	properties					778:787	Morphological, structural, thermal, optical and viscometric properties	718:787	Morphological, structural, thermal, optical and viscometric properties	718:787	Morphological, structural, thermal, optical and viscometric properties were investigated as a function of the number of passages of refined/bleached suspensions through a defibrillator.
26716240	4	1	theme	industrial	522:531	arg1	processes					533:541	industrial processes	522:541	industrial processes	522:541	The influence of industrial processes, such as pulping and refining of unbleached pulps, as well as of alkali pretreatments and bleaching of refined pulps, on the chemical composition of the samples was analyzed.
26716240	4	1	theme	industrial	522:531	arg1	refining					564:571	refining	564:571	refining	564:571	The influence of industrial processes, such as pulping and refining of unbleached pulps, as well as of alkali pretreatments and bleaching of refined pulps, on the chemical composition of the samples was analyzed.
26716240	4	1	theme	industrial	522:531	arg1	pulping					552:558	pulping	552:558	pulping	552:558	The influence of industrial processes, such as pulping and refining of unbleached pulps, as well as of alkali pretreatments and bleaching of refined pulps, on the chemical composition of the samples was analyzed.
26716240	9	2	theme	present	1728:1734	arg1	study					1736:1740	The present study	1724:1740	The present study	1724:1740	The present study contributed to the different methods that are available for the production of bamboo cellulose nanofibrils, which can be used in the production of biodegradable composites for various applications.
26716240	9	3	used	used	1863:1866	arg2	production					1806:1815	the production	1802:1815	the production	1802:1815	The present study contributed to the different methods that are available for the production of bamboo cellulose nanofibrils, which can be used in the production of biodegradable composites for various applications.
26716240	8	4	from	increase	1392:1399	arg1	efficiency					1419:1428	the production efficiency	1404:1428	the production efficiency of nanofibrils	1404:1443	The study showed that, in addition to the effectiveness of the pre-treatments, there was an increase in the production efficiency of nanofibrils, as well as in the transparency of the bleached suspensions, while viscosity, thermal stability and crystallinity had reduced levels as the number of passages through the defibrillator increased, showing a gradual improvement in the transition from the micro- to the nano-scale.
26716240	8	4	from	increase	1392:1399	arg1	transparency					1464:1475	the transparency	1460:1475	the transparency of the bleached suspensions	1460:1503	The study showed that, in addition to the effectiveness of the pre-treatments, there was an increase in the production efficiency of nanofibrils, as well as in the transparency of the bleached suspensions, while viscosity, thermal stability and crystallinity had reduced levels as the number of passages through the defibrillator increased, showing a gradual improvement in the transition from the micro- to the nano-scale.
26716240	1	5	theme	cellulose	166:174	arg1	nanofibrils					176:186	cellulose nanofibrils	166:186	cellulose nanofibrils from renewable sources for various industrial applications	166:245	There is a growing interest in cellulose nanofibrils from renewable sources for various industrial applications.
26716240	8	6	from	micro-	1698:1703	arg1	improvement					1659:1669	a gradual improvement	1649:1669	a gradual improvement in the transition from the micro- to the nano-scale	1649:1721	The study showed that, in addition to the effectiveness of the pre-treatments, there was an increase in the production efficiency of nanofibrils, as well as in the transparency of the bleached suspensions, while viscosity, thermal stability and crystallinity had reduced levels as the number of passages through the defibrillator increased, showing a gradual improvement in the transition from the micro- to the nano-scale.
26716240	8	6	from	micro-	1698:1703	arg1	transition					1678:1687	the transition	1674:1687	the transition from the micro- to the nano-scale	1674:1721	The study showed that, in addition to the effectiveness of the pre-treatments, there was an increase in the production efficiency of nanofibrils, as well as in the transparency of the bleached suspensions, while viscosity, thermal stability and crystallinity had reduced levels as the number of passages through the defibrillator increased, showing a gradual improvement in the transition from the micro- to the nano-scale.
26716240	5	7	theme	structural	733:742	arg1	function					812:819	a function	810:819	a function of the number of passages of refined/bleached suspensions through a defibrillator	810:901	Morphological, structural, thermal, optical and viscometric properties were investigated as a function of the number of passages of refined/bleached suspensions through a defibrillator.
26716240	5	7	theme	structural	733:742	arg1	properties					778:787	Morphological, structural, thermal, optical and viscometric properties	718:787	Morphological, structural, thermal, optical and viscometric properties	718:787	Morphological, structural, thermal, optical and viscometric properties were investigated as a function of the number of passages of refined/bleached suspensions through a defibrillator.
26716240	8	8	theme	nanofibrils	1433:1443	arg1	efficiency					1419:1428	the production efficiency	1404:1428	the production efficiency of nanofibrils	1404:1443	The study showed that, in addition to the effectiveness of the pre-treatments, there was an increase in the production efficiency of nanofibrils, as well as in the transparency of the bleached suspensions, while viscosity, thermal stability and crystallinity had reduced levels as the number of passages through the defibrillator increased, showing a gradual improvement in the transition from the micro- to the nano-scale.
26716240	8	9	theme	pre-treatments	1363:1376	arg1	effectiveness					1342:1354	the effectiveness	1338:1354	the effectiveness of the pre-treatments	1338:1376	The study showed that, in addition to the effectiveness of the pre-treatments, there was an increase in the production efficiency of nanofibrils, as well as in the transparency of the bleached suspensions, while viscosity, thermal stability and crystallinity had reduced levels as the number of passages through the defibrillator increased, showing a gradual improvement in the transition from the micro- to the nano-scale.
26716240	9	10	theme	composites	1903:1912	arg1	production					1875:1884	the production	1871:1884	the production of biodegradable composites for various applications	1871:1937	The present study contributed to the different methods that are available for the production of bamboo cellulose nanofibrils, which can be used in the production of biodegradable composites for various applications.
26716240	8	11	theme	gradual	1651:1657	arg1	improvement					1659:1669	a gradual improvement	1649:1669	a gradual improvement in the transition from the micro- to the nano-scale	1649:1721	The study showed that, in addition to the effectiveness of the pre-treatments, there was an increase in the production efficiency of nanofibrils, as well as in the transparency of the bleached suspensions, while viscosity, thermal stability and crystallinity had reduced levels as the number of passages through the defibrillator increased, showing a gradual improvement in the transition from the micro- to the nano-scale.
26716240	6	12	theme	unbleached	912:921	arg1	suspensions					923:933	the unbleached suspensions	908:933	the unbleached suspensions	908:933	For the unbleached suspensions, the effects of refining and bleaching on the properties of nanofibrils were evaluated, fixing the number of passages through the defibrillator.
26716240	6	13	theme	nanofibrils	995:1005	arg1	properties					981:990	the properties	977:990	the properties of nanofibrils	977:1005	For the unbleached suspensions, the effects of refining and bleaching on the properties of nanofibrils were evaluated, fixing the number of passages through the defibrillator.
26716240	5	14	theme	thermal	745:751	arg1	function					812:819	a function	810:819	a function of the number of passages of refined/bleached suspensions through a defibrillator	810:901	Morphological, structural, thermal, optical and viscometric properties were investigated as a function of the number of passages of refined/bleached suspensions through a defibrillator.
26716240	5	14	theme	thermal	745:751	arg1	properties					778:787	Morphological, structural, thermal, optical and viscometric properties	718:787	Morphological, structural, thermal, optical and viscometric properties	718:787	Morphological, structural, thermal, optical and viscometric properties were investigated as a function of the number of passages of refined/bleached suspensions through a defibrillator.
26716240	0	15	from	Pulp	49:52	arg1	Preparation					0:10	Preparation	0:10	Preparation of Cellulose Nanofibrils from Bamboo Pulp by Mechanical Defibrillation for Their Applications in Biodegradable Composites.	0:133	Preparation of Cellulose Nanofibrils from Bamboo Pulp by Mechanical Defibrillation for Their Applications in Biodegradable Composites.
26716240	5	16	theme	passages	838:845	arg1	number					828:833	the number	824:833	the number of passages of refined/bleached suspensions through a defibrillator	824:901	Morphological, structural, thermal, optical and viscometric properties were investigated as a function of the number of passages of refined/bleached suspensions through a defibrillator.
26716240	1	17	from	interest	154:161	arg1	nanofibrils					176:186	cellulose nanofibrils	166:186	cellulose nanofibrils from renewable sources for various industrial applications	166:245	There is a growing interest in cellulose nanofibrils from renewable sources for various industrial applications.
26716240	1	17	from	interest	154:161	arg1	sources					203:209	renewable sources	193:209	renewable sources for various industrial applications	193:245	There is a growing interest in cellulose nanofibrils from renewable sources for various industrial applications.
26716240	9	18	theme	cellulose	1827:1835	arg1	nanofibrils					1837:1847	bamboo cellulose nanofibrils	1820:1847	bamboo cellulose nanofibrils	1820:1847	The present study contributed to the different methods that are available for the production of bamboo cellulose nanofibrils, which can be used in the production of biodegradable composites for various applications.
26716240	5	19	theme	suspensions	867:877	arg1	passages					838:845	passages	838:845	passages of refined/bleached suspensions through a defibrillator	838:901	Morphological, structural, thermal, optical and viscometric properties were investigated as a function of the number of passages of refined/bleached suspensions through a defibrillator.
26716240	0	20	theme	Biodegradable	109:121	arg1	Composites					123:132	Biodegradable Composites	109:132	Biodegradable Composites	109:132	Preparation of Cellulose Nanofibrils from Bamboo Pulp by Mechanical Defibrillation for Their Applications in Biodegradable Composites.
26716240	4	21	from	influence	509:517	arg1	composition					677:687	the chemical composition	664:687	the chemical composition of the samples	664:702	The influence of industrial processes, such as pulping and refining of unbleached pulps, as well as of alkali pretreatments and bleaching of refined pulps, on the chemical composition of the samples was analyzed.
26716240	2	22	theme	information	276:286	arg1	lack					268:271	a lack	266:271	a lack of information on cellulose arising from bamboo pulps	266:325	However, there is a lack of information on cellulose arising from bamboo pulps.
26716240	7	23	theme	refined/bleached	1204:1219	arg1	pulp					1244:1247	the refined/bleached and refined/unbleached pulp	1200:1247	the refined/bleached and refined/unbleached pulp	1200:1247	Microscopic studies demonstrated that nanoscale cellulose fibers were obtained from both pulps, with a higher yield for the refined/bleached and refined/unbleached pulp, at the expense of the unbleached/unrefined pulps.
26716240	5	24	theme	refined/bleached	850:865	arg1	suspensions					867:877	refined/bleached suspensions	850:877	refined/bleached suspensions through a defibrillator	850:901	Morphological, structural, thermal, optical and viscometric properties were investigated as a function of the number of passages of refined/bleached suspensions through a defibrillator.
26716240	8	25	theme	passages	1595:1602	arg1	number					1585:1590	the number	1581:1590	the number of passages through the defibrillator	1581:1628	The study showed that, in addition to the effectiveness of the pre-treatments, there was an increase in the production efficiency of nanofibrils, as well as in the transparency of the bleached suspensions, while viscosity, thermal stability and crystallinity had reduced levels as the number of passages through the defibrillator increased, showing a gradual improvement in the transition from the micro- to the nano-scale.
26716240	5	26	theme	optical	754:760	arg1	function					812:819	a function	810:819	a function of the number of passages of refined/bleached suspensions through a defibrillator	810:901	Morphological, structural, thermal, optical and viscometric properties were investigated as a function of the number of passages of refined/bleached suspensions through a defibrillator.
26716240	5	26	theme	optical	754:760	arg1	properties					778:787	Morphological, structural, thermal, optical and viscometric properties	718:787	Morphological, structural, thermal, optical and viscometric properties	718:787	Morphological, structural, thermal, optical and viscometric properties were investigated as a function of the number of passages of refined/bleached suspensions through a defibrillator.
26716240	8	27	theme	thermal	1523:1529	arg1	stability					1531:1539	thermal stability	1523:1539	thermal stability	1523:1539	The study showed that, in addition to the effectiveness of the pre-treatments, there was an increase in the production efficiency of nanofibrils, as well as in the transparency of the bleached suspensions, while viscosity, thermal stability and crystallinity had reduced levels as the number of passages through the defibrillator increased, showing a gradual improvement in the transition from the micro- to the nano-scale.
26716240	1	28	theme	renewable	193:201	arg1	sources					203:209	renewable sources	193:209	renewable sources for various industrial applications	193:245	There is a growing interest in cellulose nanofibrils from renewable sources for various industrial applications.
26716240	4	29	theme	unbleached	576:585	arg1	pulps					587:591	unbleached pulps	576:591	unbleached pulps	576:591	The influence of industrial processes, such as pulping and refining of unbleached pulps, as well as of alkali pretreatments and bleaching of refined pulps, on the chemical composition of the samples was analyzed.
26716240	7	30	theme	nanoscale	1118:1126	arg1	fibers					1138:1143	nanoscale cellulose fibers	1118:1143	nanoscale cellulose fibers	1118:1143	Microscopic studies demonstrated that nanoscale cellulose fibers were obtained from both pulps, with a higher yield for the refined/bleached and refined/unbleached pulp, at the expense of the unbleached/unrefined pulps.
26716240	7	31	theme	cellulose	1128:1136	arg1	fibers					1138:1143	nanoscale cellulose fibers	1118:1143	nanoscale cellulose fibers	1118:1143	Microscopic studies demonstrated that nanoscale cellulose fibers were obtained from both pulps, with a higher yield for the refined/bleached and refined/unbleached pulp, at the expense of the unbleached/unrefined pulps.
26716240	3	32	theme	mechanical	442:451	arg1	defibrillation					453:466	mechanical defibrillation	442:466	mechanical defibrillation	442:466	Nanofibrils from refined bamboo pulps, including bleached, unbleached, and unrefined/unbleached, were obtained by mechanical defibrillation for use in biodegradable composites.
26716240	0	33	theme	Nanofibrils	25:35	arg1	Preparation					0:10	Preparation	0:10	Preparation of Cellulose Nanofibrils from Bamboo Pulp by Mechanical Defibrillation for Their Applications in Biodegradable Composites.	0:133	Preparation of Cellulose Nanofibrils from Bamboo Pulp by Mechanical Defibrillation for Their Applications in Biodegradable Composites.
26716240	9	34	theme	bamboo	1820:1825	arg1	nanofibrils					1837:1847	bamboo cellulose nanofibrils	1820:1847	bamboo cellulose nanofibrils	1820:1847	The present study contributed to the different methods that are available for the production of bamboo cellulose nanofibrils, which can be used in the production of biodegradable composites for various applications.
26716240	2	35	theme	bamboo	314:319	arg1	pulps					321:325	bamboo pulps	314:325	bamboo pulps	314:325	However, there is a lack of information on cellulose arising from bamboo pulps.
26716240	3	36	theme	biodegradable	479:491	arg1	composites					493:502	biodegradable composites	479:502	biodegradable composites	479:502	Nanofibrils from refined bamboo pulps, including bleached, unbleached, and unrefined/unbleached, were obtained by mechanical defibrillation for use in biodegradable composites.
26716240	0	37	theme	Cellulose	15:23	arg1	Nanofibrils					25:35	Cellulose Nanofibrils	15:35	Cellulose Nanofibrils	15:35	Preparation of Cellulose Nanofibrils from Bamboo Pulp by Mechanical Defibrillation for Their Applications in Biodegradable Composites.
26716240	3	38	from	pulps	360:364	arg1	Nanofibrils					328:338	Nanofibrils	328:338	Nanofibrils from refined bamboo pulps, including bleached, unbleached, and unrefined/unbleached,	328:423	Nanofibrils from refined bamboo pulps, including bleached, unbleached, and unrefined/unbleached, were obtained by mechanical defibrillation for use in biodegradable composites.
26716240	4	39	theme	samples	696:702	arg1	composition					677:687	the chemical composition	664:687	the chemical composition of the samples	664:702	The influence of industrial processes, such as pulping and refining of unbleached pulps, as well as of alkali pretreatments and bleaching of refined pulps, on the chemical composition of the samples was analyzed.
26716240	1	40	theme	various	215:221	arg1	applications					234:245	various industrial applications	215:245	various industrial applications	215:245	There is a growing interest in cellulose nanofibrils from renewable sources for various industrial applications.
26716240	0	41	theme	Bamboo	42:47	arg1	Pulp					49:52	Bamboo Pulp	42:52	Bamboo Pulp	42:52	Preparation of Cellulose Nanofibrils from Bamboo Pulp by Mechanical Defibrillation for Their Applications in Biodegradable Composites.
26716240	9	42	theme	various	1918:1924	arg1	applications					1926:1937	various applications	1918:1937	various applications	1918:1937	The present study contributed to the different methods that are available for the production of bamboo cellulose nanofibrils, which can be used in the production of biodegradable composites for various applications.
26716240	5	43	theme	Morphological	718:730	arg1	function					812:819	a function	810:819	a function of the number of passages of refined/bleached suspensions through a defibrillator	810:901	Morphological, structural, thermal, optical and viscometric properties were investigated as a function of the number of passages of refined/bleached suspensions through a defibrillator.
26716240	5	43	theme	Morphological	718:730	arg1	properties					778:787	Morphological, structural, thermal, optical and viscometric properties	718:787	Morphological, structural, thermal, optical and viscometric properties	718:787	Morphological, structural, thermal, optical and viscometric properties were investigated as a function of the number of passages of refined/bleached suspensions through a defibrillator.
26716240	7	44	theme	unbleached/unrefined	1272:1291	arg1	pulps					1293:1297	the unbleached/unrefined pulps	1268:1297	the unbleached/unrefined pulps	1268:1297	Microscopic studies demonstrated that nanoscale cellulose fibers were obtained from both pulps, with a higher yield for the refined/bleached and refined/unbleached pulp, at the expense of the unbleached/unrefined pulps.
26716240	8	45	theme	production	1408:1417	arg1	efficiency					1419:1428	the production efficiency	1404:1428	the production efficiency of nanofibrils	1404:1443	The study showed that, in addition to the effectiveness of the pre-treatments, there was an increase in the production efficiency of nanofibrils, as well as in the transparency of the bleached suspensions, while viscosity, thermal stability and crystallinity had reduced levels as the number of passages through the defibrillator increased, showing a gradual improvement in the transition from the micro- to the nano-scale.
26716240	2	46	from	lack	268:271	arg1	cellulose					291:299	cellulose	291:299	cellulose arising from bamboo pulps	291:325	However, there is a lack of information on cellulose arising from bamboo pulps.
26716240	1	47	theme	industrial	223:232	arg1	applications					234:245	various industrial applications	215:245	various industrial applications	215:245	There is a growing interest in cellulose nanofibrils from renewable sources for various industrial applications.
26716240	6	48	theme	bleaching	964:972	arg1	effects					940:946	the effects	936:946	the effects of refining and bleaching on the properties of nanofibrils	936:1005	For the unbleached suspensions, the effects of refining and bleaching on the properties of nanofibrils were evaluated, fixing the number of passages through the defibrillator.
26716240	7	49	theme	Microscopic	1080:1090	arg1	studies					1092:1098	Microscopic studies	1080:1098	Microscopic studies	1080:1098	Microscopic studies demonstrated that nanoscale cellulose fibers were obtained from both pulps, with a higher yield for the refined/bleached and refined/unbleached pulp, at the expense of the unbleached/unrefined pulps.
26716240	7	50	theme	higher	1183:1188	arg1	yield					1190:1194	a higher yield	1181:1194	a higher yield for the refined/bleached and refined/unbleached pulp	1181:1247	Microscopic studies demonstrated that nanoscale cellulose fibers were obtained from both pulps, with a higher yield for the refined/bleached and refined/unbleached pulp, at the expense of the unbleached/unrefined pulps.
26716240	3	51	from	use	472:474	arg1	composites					493:502	biodegradable composites	479:502	biodegradable composites	479:502	Nanofibrils from refined bamboo pulps, including bleached, unbleached, and unrefined/unbleached, were obtained by mechanical defibrillation for use in biodegradable composites.
26716240	4	52	theme	pulps	654:658	arg1	pretreatments					615:627	alkali pretreatments	608:627	alkali pretreatments	608:627	The influence of industrial processes, such as pulping and refining of unbleached pulps, as well as of alkali pretreatments and bleaching of refined pulps, on the chemical composition of the samples was analyzed.
26716240	4	52	theme	pulps	654:658	arg1	bleaching					633:641	bleaching	633:641	bleaching of refined pulps	633:658	The influence of industrial processes, such as pulping and refining of unbleached pulps, as well as of alkali pretreatments and bleaching of refined pulps, on the chemical composition of the samples was analyzed.
26716240	7	53	theme	refined/unbleached	1225:1242	arg1	pulp					1244:1247	the refined/bleached and refined/unbleached pulp	1200:1247	the refined/bleached and refined/unbleached pulp	1200:1247	Microscopic studies demonstrated that nanoscale cellulose fibers were obtained from both pulps, with a higher yield for the refined/bleached and refined/unbleached pulp, at the expense of the unbleached/unrefined pulps.
26716240	6	54	theme	passages	1044:1051	arg1	number					1034:1039	the number	1030:1039	the number of passages	1030:1051	For the unbleached suspensions, the effects of refining and bleaching on the properties of nanofibrils were evaluated, fixing the number of passages through the defibrillator.
26716240	0	55	theme	Mechanical	57:66	arg1	Defibrillation					68:81	Mechanical Defibrillation	57:81	Mechanical Defibrillation	57:81	Preparation of Cellulose Nanofibrils from Bamboo Pulp by Mechanical Defibrillation for Their Applications in Biodegradable Composites.
26716240	8	56	theme	bleached	1484:1491	arg1	suspensions					1493:1503	the bleached suspensions	1480:1503	the bleached suspensions	1480:1503	The study showed that, in addition to the effectiveness of the pre-treatments, there was an increase in the production efficiency of nanofibrils, as well as in the transparency of the bleached suspensions, while viscosity, thermal stability and crystallinity had reduced levels as the number of passages through the defibrillator increased, showing a gradual improvement in the transition from the micro- to the nano-scale.
26716240	6	57	theme	refining	951:958	arg1	effects					940:946	the effects	936:946	the effects of refining and bleaching on the properties of nanofibrils	936:1005	For the unbleached suspensions, the effects of refining and bleaching on the properties of nanofibrils were evaluated, fixing the number of passages through the defibrillator.
26716240	4	58	theme	chemical	668:675	arg1	composition					677:687	the chemical composition	664:687	the chemical composition of the samples	664:702	The influence of industrial processes, such as pulping and refining of unbleached pulps, as well as of alkali pretreatments and bleaching of refined pulps, on the chemical composition of the samples was analyzed.
26716240	9	59	theme	biodegradable	1889:1901	arg1	composites					1903:1912	biodegradable composites	1889:1912	biodegradable composites for various applications	1889:1937	The present study contributed to the different methods that are available for the production of bamboo cellulose nanofibrils, which can be used in the production of biodegradable composites for various applications.
26716240	8	60	theme	reduced	1563:1569	arg1	levels					1571:1576	reduced levels	1563:1576	reduced levels	1563:1576	The study showed that, in addition to the effectiveness of the pre-treatments, there was an increase in the production efficiency of nanofibrils, as well as in the transparency of the bleached suspensions, while viscosity, thermal stability and crystallinity had reduced levels as the number of passages through the defibrillator increased, showing a gradual improvement in the transition from the micro- to the nano-scale.
26716240	8	61	dep	effectiveness	1342:1354	arg1	addition					1326:1333	addition	1326:1333	addition	1326:1333	The study showed that, in addition to the effectiveness of the pre-treatments, there was an increase in the production efficiency of nanofibrils, as well as in the transparency of the bleached suspensions, while viscosity, thermal stability and crystallinity had reduced levels as the number of passages through the defibrillator increased, showing a gradual improvement in the transition from the micro- to the nano-scale.
26716240	8	62	from	improvement	1659:1669	arg1	transition					1678:1687	the transition	1674:1687	the transition from the micro- to the nano-scale	1674:1721	The study showed that, in addition to the effectiveness of the pre-treatments, there was an increase in the production efficiency of nanofibrils, as well as in the transparency of the bleached suspensions, while viscosity, thermal stability and crystallinity had reduced levels as the number of passages through the defibrillator increased, showing a gradual improvement in the transition from the micro- to the nano-scale.
26716240	8	62	from	improvement	1659:1669	arg1	micro-					1698:1703	the micro-	1694:1703	the micro-	1694:1703	The study showed that, in addition to the effectiveness of the pre-treatments, there was an increase in the production efficiency of nanofibrils, as well as in the transparency of the bleached suspensions, while viscosity, thermal stability and crystallinity had reduced levels as the number of passages through the defibrillator increased, showing a gradual improvement in the transition from the micro- to the nano-scale.
26716240	8	63	theme	suspensions	1493:1503	arg1	transparency					1464:1475	the transparency	1460:1475	the transparency of the bleached suspensions	1460:1503	The study showed that, in addition to the effectiveness of the pre-treatments, there was an increase in the production efficiency of nanofibrils, as well as in the transparency of the bleached suspensions, while viscosity, thermal stability and crystallinity had reduced levels as the number of passages through the defibrillator increased, showing a gradual improvement in the transition from the micro- to the nano-scale.
26716240	5	64	theme	number	828:833	arg1	function					812:819	a function	810:819	a function of the number of passages of refined/bleached suspensions through a defibrillator	810:901	Morphological, structural, thermal, optical and viscometric properties were investigated as a function of the number of passages of refined/bleached suspensions through a defibrillator.
26716240	5	64	theme	number	828:833	arg1	properties					778:787	Morphological, structural, thermal, optical and viscometric properties	718:787	Morphological, structural, thermal, optical and viscometric properties	718:787	Morphological, structural, thermal, optical and viscometric properties were investigated as a function of the number of passages of refined/bleached suspensions through a defibrillator.
26716240	9	65	theme	nanofibrils	1837:1847	arg1	production					1806:1815	the production	1802:1815	the production	1802:1815	The present study contributed to the different methods that are available for the production of bamboo cellulose nanofibrils, which can be used in the production of biodegradable composites for various applications.
26716240	4	66	theme	alkali	608:613	arg1	pretreatments					615:627	alkali pretreatments	608:627	alkali pretreatments	608:627	The influence of industrial processes, such as pulping and refining of unbleached pulps, as well as of alkali pretreatments and bleaching of refined pulps, on the chemical composition of the samples was analyzed.
26716240	3	67	theme	refined	345:351	arg1	unrefined/unbleached					403:422	unrefined/unbleached	403:422	unrefined/unbleached	403:422	Nanofibrils from refined bamboo pulps, including bleached, unbleached, and unrefined/unbleached, were obtained by mechanical defibrillation for use in biodegradable composites.
26716240	3	67	theme	refined	345:351	arg1	unbleached					387:396	unbleached	387:396	unbleached	387:396	Nanofibrils from refined bamboo pulps, including bleached, unbleached, and unrefined/unbleached, were obtained by mechanical defibrillation for use in biodegradable composites.
26716240	3	67	theme	refined	345:351	arg1	pulps					360:364	refined bamboo pulps	345:364	refined bamboo pulps	345:364	Nanofibrils from refined bamboo pulps, including bleached, unbleached, and unrefined/unbleached, were obtained by mechanical defibrillation for use in biodegradable composites.
26716240	3	67	theme	refined	345:351	arg1	bleached					377:384	bleached	377:384	bleached	377:384	Nanofibrils from refined bamboo pulps, including bleached, unbleached, and unrefined/unbleached, were obtained by mechanical defibrillation for use in biodegradable composites.
26716240	0	68	from	Applications	93:104	arg1	Composites					123:132	Biodegradable Composites	109:132	Biodegradable Composites	109:132	Preparation of Cellulose Nanofibrils from Bamboo Pulp by Mechanical Defibrillation for Their Applications in Biodegradable Composites.
26716240	9	69	theme	different	1761:1769	arg1	methods					1771:1777	the different methods	1757:1777	the different methods that are available for the production of bamboo cellulose nanofibrils, which can be used in the production of biodegradable composites for various applications	1757:1937	The present study contributed to the different methods that are available for the production of bamboo cellulose nanofibrils, which can be used in the production of biodegradable composites for various applications.
26716240	9	69	theme	different	1761:1769	arg1	available					1788:1796	available	1788:1796	available	1788:1796	The present study contributed to the different methods that are available for the production of bamboo cellulose nanofibrils, which can be used in the production of biodegradable composites for various applications.
26716240	1	70	from	sources	203:209	arg1	interest					154:161	a growing interest	144:161	a growing interest in cellulose nanofibrils from renewable sources for various industrial applications	144:245	There is a growing interest in cellulose nanofibrils from renewable sources for various industrial applications.
26716240	1	70	from	sources	203:209	arg1	nanofibrils					176:186	cellulose nanofibrils	166:186	cellulose nanofibrils from renewable sources for various industrial applications	166:245	There is a growing interest in cellulose nanofibrils from renewable sources for various industrial applications.
26716240	1	71	theme	growing	146:152	arg1	interest					154:161	a growing interest	144:161	a growing interest in cellulose nanofibrils from renewable sources for various industrial applications	144:245	There is a growing interest in cellulose nanofibrils from renewable sources for various industrial applications.
26716240	3	72	theme	bamboo	353:358	arg1	unrefined/unbleached					403:422	unrefined/unbleached	403:422	unrefined/unbleached	403:422	Nanofibrils from refined bamboo pulps, including bleached, unbleached, and unrefined/unbleached, were obtained by mechanical defibrillation for use in biodegradable composites.
26716240	3	72	theme	bamboo	353:358	arg1	unbleached					387:396	unbleached	387:396	unbleached	387:396	Nanofibrils from refined bamboo pulps, including bleached, unbleached, and unrefined/unbleached, were obtained by mechanical defibrillation for use in biodegradable composites.
26716240	3	72	theme	bamboo	353:358	arg1	pulps					360:364	refined bamboo pulps	345:364	refined bamboo pulps	345:364	Nanofibrils from refined bamboo pulps, including bleached, unbleached, and unrefined/unbleached, were obtained by mechanical defibrillation for use in biodegradable composites.
26716240	3	72	theme	bamboo	353:358	arg1	bleached					377:384	bleached	377:384	bleached	377:384	Nanofibrils from refined bamboo pulps, including bleached, unbleached, and unrefined/unbleached, were obtained by mechanical defibrillation for use in biodegradable composites.
26716240	4	73	theme	processes	533:541	arg1	influence					509:517	The influence	505:517	The influence of industrial processes, such as pulping and refining of unbleached pulps, as well as of alkali pretreatments and bleaching of refined pulps, on the chemical composition of the samples	505:702	The influence of industrial processes, such as pulping and refining of unbleached pulps, as well as of alkali pretreatments and bleaching of refined pulps, on the chemical composition of the samples was analyzed.
26716240	4	74	theme	refined	646:652	arg1	pulps					654:658	refined pulps	646:658	refined pulps	646:658	The influence of industrial processes, such as pulping and refining of unbleached pulps, as well as of alkali pretreatments and bleaching of refined pulps, on the chemical composition of the samples was analyzed.
26716240	8	75	contain	had	1559:1561	arg1	viscosity					1512:1520	viscosity	1512:1520	viscosity	1512:1520	The study showed that, in addition to the effectiveness of the pre-treatments, there was an increase in the production efficiency of nanofibrils, as well as in the transparency of the bleached suspensions, while viscosity, thermal stability and crystallinity had reduced levels as the number of passages through the defibrillator increased, showing a gradual improvement in the transition from the micro- to the nano-scale.
26716240	8	75	contain	had	1559:1561	arg1	stability					1531:1539	thermal stability	1523:1539	thermal stability	1523:1539	The study showed that, in addition to the effectiveness of the pre-treatments, there was an increase in the production efficiency of nanofibrils, as well as in the transparency of the bleached suspensions, while viscosity, thermal stability and crystallinity had reduced levels as the number of passages through the defibrillator increased, showing a gradual improvement in the transition from the micro- to the nano-scale.
26716240	8	75	contain	had	1559:1561	arg1	crystallinity					1545:1557	crystallinity	1545:1557	crystallinity	1545:1557	The study showed that, in addition to the effectiveness of the pre-treatments, there was an increase in the production efficiency of nanofibrils, as well as in the transparency of the bleached suspensions, while viscosity, thermal stability and crystallinity had reduced levels as the number of passages through the defibrillator increased, showing a gradual improvement in the transition from the micro- to the nano-scale.
26716240	8	75	contain	had	1559:1561	arg2	levels					1571:1576	reduced levels	1563:1576	reduced levels	1563:1576	The study showed that, in addition to the effectiveness of the pre-treatments, there was an increase in the production efficiency of nanofibrils, as well as in the transparency of the bleached suspensions, while viscosity, thermal stability and crystallinity had reduced levels as the number of passages through the defibrillator increased, showing a gradual improvement in the transition from the micro- to the nano-scale.
26716240	7	76	theme	pulps	1293:1297	arg1	expense					1257:1263	the expense	1253:1263	the expense of the unbleached/unrefined pulps	1253:1297	Microscopic studies demonstrated that nanoscale cellulose fibers were obtained from both pulps, with a higher yield for the refined/bleached and refined/unbleached pulp, at the expense of the unbleached/unrefined pulps.
26716240	6	77	from	effects	940:946	arg1	properties					981:990	the properties	977:990	the properties of nanofibrils	977:1005	For the unbleached suspensions, the effects of refining and bleaching on the properties of nanofibrils were evaluated, fixing the number of passages through the defibrillator.
28490053	0	0	theme	fungal	82:87	arg1	xylanase					94:101	a novel fungal GH11 xylanase	74:101	a novel fungal GH11 xylanase	74:101	Prebiotic effect of xylooligosaccharides produced from birchwood xylan by a novel fungal GH11 xylanase.
28490053	3	1	contain	contained	454:462	arg2	amount					477:482	a negligible amount	464:482	a negligible amount of xylose	464:492	A notable yield of neutral XOS was obtained (28.8%) upon enzyme treatment and the mixture contained a negligible amount of xylose, having xylobiose, xylotriose and xylotetraose as its main components.
28490053	3	1	contain	contained	454:462	arg1	mixture					446:452	the mixture	442:452	the mixture	442:452	A notable yield of neutral XOS was obtained (28.8%) upon enzyme treatment and the mixture contained a negligible amount of xylose, having xylobiose, xylotriose and xylotetraose as its main components.
28490053	3	1	contain	contained	454:462	arg2	xylose					487:492	xylose	487:492	xylose	487:492	A notable yield of neutral XOS was obtained (28.8%) upon enzyme treatment and the mixture contained a negligible amount of xylose, having xylobiose, xylotriose and xylotetraose as its main components.
28490053	1	2	theme	hydrolase	153:161	arg1	family					168:173	the glycoside hydrolase gene family 11 (GH11)	139:183	the glycoside hydrolase gene family 11 (GH11)	139:183	A fungal endoxylanase belonging to the glycoside hydrolase gene family 11 (GH11) was obtained from the ascomycete Talaromyces amestolkiae.
28490053	0	3	theme	novel	76:80	arg1	xylanase					94:101	a novel fungal GH11 xylanase	74:101	a novel fungal GH11 xylanase	74:101	Prebiotic effect of xylooligosaccharides produced from birchwood xylan by a novel fungal GH11 xylanase.
28490053	1	4	theme	gene	163:166	arg1	family					168:173	the glycoside hydrolase gene family 11 (GH11)	139:183	the glycoside hydrolase gene family 11 (GH11)	139:183	A fungal endoxylanase belonging to the glycoside hydrolase gene family 11 (GH11) was obtained from the ascomycete Talaromyces amestolkiae.
28490053	4	5	from	variations	641:650	arg1	composition					671:681	microorganisms' composition	655:681	microorganisms' composition	655:681	The prebiotic potential of this mixture was demonstrated upon analyzing the variations in microorganisms' composition and organic acids profile in breast-fed child faeces fermentations.
28490053	4	5	from	variations	641:650	arg1	acids					695:699	organic acids	687:699	organic acids	687:699	The prebiotic potential of this mixture was demonstrated upon analyzing the variations in microorganisms' composition and organic acids profile in breast-fed child faeces fermentations.
28490053	4	5	from	variations	641:650	arg1	fermentations					736:748	breast-fed child faeces fermentations	712:748	breast-fed child faeces fermentations	712:748	The prebiotic potential of this mixture was demonstrated upon analyzing the variations in microorganisms' composition and organic acids profile in breast-fed child faeces fermentations.
28490053	3	6	theme	notable	366:372	arg1	yield					374:378	A notable yield	364:378	A notable yield of neutral XOS	364:393	A notable yield of neutral XOS was obtained (28.8%) upon enzyme treatment and the mixture contained a negligible amount of xylose, having xylobiose, xylotriose and xylotetraose as its main components.
28490053	2	7	from	mixture	304:310	arg1	xylan					357:361	birchwood xylan	347:361	birchwood xylan	347:361	The enzyme was purified, characterized and used to produce a mixture of xylooligosaccharides (XOS) from birchwood xylan.
28490053	4	8	theme	faeces	729:734	arg1	fermentations					736:748	breast-fed child faeces fermentations	712:748	breast-fed child faeces fermentations	712:748	The prebiotic potential of this mixture was demonstrated upon analyzing the variations in microorganisms' composition and organic acids profile in breast-fed child faeces fermentations.
28490053	0	9	theme	GH11	89:92	arg1	xylanase					94:101	a novel fungal GH11 xylanase	74:101	a novel fungal GH11 xylanase	74:101	Prebiotic effect of xylooligosaccharides produced from birchwood xylan by a novel fungal GH11 xylanase.
28490053	4	10	theme	child	723:727	arg1	fermentations					736:748	breast-fed child faeces fermentations	712:748	breast-fed child faeces fermentations	712:748	The prebiotic potential of this mixture was demonstrated upon analyzing the variations in microorganisms' composition and organic acids profile in breast-fed child faeces fermentations.
28490053	5	11	theme	possible	888:895	arg1	commensals					908:917	possible beneficial commensals	888:917	possible beneficial commensals	888:917	The strong production of acetic and lactic acid, the decrease of potentially pathogenic bacteria and the increase of bifidobacteria, and possible beneficial commensals, confirmed the prebiotic value of these xylooligosaccharides.
28490053	3	12	theme	xylose	487:492	arg1	xylose					487:492	xylose	487:492	xylose	487:492	A notable yield of neutral XOS was obtained (28.8%) upon enzyme treatment and the mixture contained a negligible amount of xylose, having xylobiose, xylotriose and xylotetraose as its main components.
28490053	3	12	theme	xylose	487:492	arg1	amount					477:482	a negligible amount	464:482	a negligible amount of xylose	464:492	A notable yield of neutral XOS was obtained (28.8%) upon enzyme treatment and the mixture contained a negligible amount of xylose, having xylobiose, xylotriose and xylotetraose as its main components.
28490053	5	13	theme	pathogenic	828:837	arg1	bacteria					839:846	potentially pathogenic bacteria	816:846	potentially pathogenic bacteria	816:846	The strong production of acetic and lactic acid, the decrease of potentially pathogenic bacteria and the increase of bifidobacteria, and possible beneficial commensals, confirmed the prebiotic value of these xylooligosaccharides.
28490053	5	14	theme	acetic	776:781	arg1	decrease					804:811	the decrease	800:811	the decrease of potentially pathogenic bacteria	800:846	The strong production of acetic and lactic acid, the decrease of potentially pathogenic bacteria and the increase of bifidobacteria, and possible beneficial commensals, confirmed the prebiotic value of these xylooligosaccharides.
28490053	5	14	theme	acetic	776:781	arg1	increase					856:863	the increase	852:863	the increase of bifidobacteria, and possible beneficial commensals	852:917	The strong production of acetic and lactic acid, the decrease of potentially pathogenic bacteria and the increase of bifidobacteria, and possible beneficial commensals, confirmed the prebiotic value of these xylooligosaccharides.
28490053	5	14	theme	acetic	776:781	arg1	acid					794:797	acetic and lactic acid	776:797	acetic and lactic acid	776:797	The strong production of acetic and lactic acid, the decrease of potentially pathogenic bacteria and the increase of bifidobacteria, and possible beneficial commensals, confirmed the prebiotic value of these xylooligosaccharides.
28490053	5	15	theme	commensals	908:917	arg1	decrease					804:811	the decrease	800:811	the decrease of potentially pathogenic bacteria	800:846	The strong production of acetic and lactic acid, the decrease of potentially pathogenic bacteria and the increase of bifidobacteria, and possible beneficial commensals, confirmed the prebiotic value of these xylooligosaccharides.
28490053	5	15	theme	commensals	908:917	arg1	increase					856:863	the increase	852:863	the increase of bifidobacteria, and possible beneficial commensals	852:917	The strong production of acetic and lactic acid, the decrease of potentially pathogenic bacteria and the increase of bifidobacteria, and possible beneficial commensals, confirmed the prebiotic value of these xylooligosaccharides.
28490053	5	15	theme	commensals	908:917	arg1	acid					794:797	acetic and lactic acid	776:797	acetic and lactic acid	776:797	The strong production of acetic and lactic acid, the decrease of potentially pathogenic bacteria and the increase of bifidobacteria, and possible beneficial commensals, confirmed the prebiotic value of these xylooligosaccharides.
28490053	3	16	theme	negligible	466:475	arg1	xylose					487:492	xylose	487:492	xylose	487:492	A notable yield of neutral XOS was obtained (28.8%) upon enzyme treatment and the mixture contained a negligible amount of xylose, having xylobiose, xylotriose and xylotetraose as its main components.
28490053	3	16	theme	negligible	466:475	arg1	amount					477:482	a negligible amount	464:482	a negligible amount of xylose	464:492	A notable yield of neutral XOS was obtained (28.8%) upon enzyme treatment and the mixture contained a negligible amount of xylose, having xylobiose, xylotriose and xylotetraose as its main components.
28490053	5	17	theme	bacteria	839:846	arg1	decrease					804:811	the decrease	800:811	the decrease of potentially pathogenic bacteria	800:846	The strong production of acetic and lactic acid, the decrease of potentially pathogenic bacteria and the increase of bifidobacteria, and possible beneficial commensals, confirmed the prebiotic value of these xylooligosaccharides.
28490053	5	17	theme	bacteria	839:846	arg1	increase					856:863	the increase	852:863	the increase of bifidobacteria, and possible beneficial commensals	852:917	The strong production of acetic and lactic acid, the decrease of potentially pathogenic bacteria and the increase of bifidobacteria, and possible beneficial commensals, confirmed the prebiotic value of these xylooligosaccharides.
28490053	5	17	theme	bacteria	839:846	arg1	acid					794:797	acetic and lactic acid	776:797	acetic and lactic acid	776:797	The strong production of acetic and lactic acid, the decrease of potentially pathogenic bacteria and the increase of bifidobacteria, and possible beneficial commensals, confirmed the prebiotic value of these xylooligosaccharides.
28490053	0	18	theme	Prebiotic	0:8	arg1	effect					10:15	Prebiotic effect	0:15	Prebiotic effect of xylooligosaccharides	0:39	Prebiotic effect of xylooligosaccharides produced from birchwood xylan by a novel fungal GH11 xylanase.
28490053	0	19	theme	xylooligosaccharides	20:39	arg1	effect					10:15	Prebiotic effect	0:15	Prebiotic effect of xylooligosaccharides	0:39	Prebiotic effect of xylooligosaccharides produced from birchwood xylan by a novel fungal GH11 xylanase.
28490053	4	20	theme	breast-fed	712:721	arg1	fermentations					736:748	breast-fed child faeces fermentations	712:748	breast-fed child faeces fermentations	712:748	The prebiotic potential of this mixture was demonstrated upon analyzing the variations in microorganisms' composition and organic acids profile in breast-fed child faeces fermentations.
28490053	5	21	theme	acid	794:797	arg1	production					762:771	The strong production	751:771	The strong production of acetic and lactic acid, the decrease of potentially pathogenic bacteria and the increase of bifidobacteria, and possible beneficial commensals,	751:918	The strong production of acetic and lactic acid, the decrease of potentially pathogenic bacteria and the increase of bifidobacteria, and possible beneficial commensals, confirmed the prebiotic value of these xylooligosaccharides.
28490053	5	22	theme	bifidobacteria	868:881	arg1	decrease					804:811	the decrease	800:811	the decrease of potentially pathogenic bacteria	800:846	The strong production of acetic and lactic acid, the decrease of potentially pathogenic bacteria and the increase of bifidobacteria, and possible beneficial commensals, confirmed the prebiotic value of these xylooligosaccharides.
28490053	5	22	theme	bifidobacteria	868:881	arg1	increase					856:863	the increase	852:863	the increase of bifidobacteria, and possible beneficial commensals	852:917	The strong production of acetic and lactic acid, the decrease of potentially pathogenic bacteria and the increase of bifidobacteria, and possible beneficial commensals, confirmed the prebiotic value of these xylooligosaccharides.
28490053	5	22	theme	bifidobacteria	868:881	arg1	acid					794:797	acetic and lactic acid	776:797	acetic and lactic acid	776:797	The strong production of acetic and lactic acid, the decrease of potentially pathogenic bacteria and the increase of bifidobacteria, and possible beneficial commensals, confirmed the prebiotic value of these xylooligosaccharides.
28490053	3	23	theme	enzyme	421:426	arg1	treatment					428:436	enzyme treatment	421:436	enzyme treatment	421:436	A notable yield of neutral XOS was obtained (28.8%) upon enzyme treatment and the mixture contained a negligible amount of xylose, having xylobiose, xylotriose and xylotetraose as its main components.
28490053	3	24	theme	neutral	383:389	arg1	XOS					391:393	neutral XOS	383:393	neutral XOS	383:393	A notable yield of neutral XOS was obtained (28.8%) upon enzyme treatment and the mixture contained a negligible amount of xylose, having xylobiose, xylotriose and xylotetraose as its main components.
28490053	4	25	theme	mixture	597:603	arg1	potential					579:587	The prebiotic potential	565:587	The prebiotic potential of this mixture	565:603	The prebiotic potential of this mixture was demonstrated upon analyzing the variations in microorganisms' composition and organic acids profile in breast-fed child faeces fermentations.
28490053	3	26	theme	XOS	391:393	arg1	yield					374:378	A notable yield	364:378	A notable yield of neutral XOS	364:393	A notable yield of neutral XOS was obtained (28.8%) upon enzyme treatment and the mixture contained a negligible amount of xylose, having xylobiose, xylotriose and xylotetraose as its main components.
28490053	5	27	theme	strong	755:760	arg1	production					762:771	The strong production	751:771	The strong production of acetic and lactic acid, the decrease of potentially pathogenic bacteria and the increase of bifidobacteria, and possible beneficial commensals,	751:918	The strong production of acetic and lactic acid, the decrease of potentially pathogenic bacteria and the increase of bifidobacteria, and possible beneficial commensals, confirmed the prebiotic value of these xylooligosaccharides.
28490053	3	28	theme	main	548:551	arg1	xylotetraose					528:539	xylotetraose	528:539	xylotetraose	528:539	A notable yield of neutral XOS was obtained (28.8%) upon enzyme treatment and the mixture contained a negligible amount of xylose, having xylobiose, xylotriose and xylotetraose as its main components.
28490053	3	28	theme	main	548:551	arg1	xylotriose					513:522	xylotriose	513:522	xylotriose	513:522	A notable yield of neutral XOS was obtained (28.8%) upon enzyme treatment and the mixture contained a negligible amount of xylose, having xylobiose, xylotriose and xylotetraose as its main components.
28490053	3	28	theme	main	548:551	arg1	xylobiose					502:510	xylobiose	502:510	xylobiose	502:510	A notable yield of neutral XOS was obtained (28.8%) upon enzyme treatment and the mixture contained a negligible amount of xylose, having xylobiose, xylotriose and xylotetraose as its main components.
28490053	3	28	theme	main	548:551	arg1	components					553:562	its main components	544:562	its main components	544:562	A notable yield of neutral XOS was obtained (28.8%) upon enzyme treatment and the mixture contained a negligible amount of xylose, having xylobiose, xylotriose and xylotetraose as its main components.
28490053	5	29	theme	lactic	787:792	arg1	decrease					804:811	the decrease	800:811	the decrease of potentially pathogenic bacteria	800:846	The strong production of acetic and lactic acid, the decrease of potentially pathogenic bacteria and the increase of bifidobacteria, and possible beneficial commensals, confirmed the prebiotic value of these xylooligosaccharides.
28490053	5	29	theme	lactic	787:792	arg1	increase					856:863	the increase	852:863	the increase of bifidobacteria, and possible beneficial commensals	852:917	The strong production of acetic and lactic acid, the decrease of potentially pathogenic bacteria and the increase of bifidobacteria, and possible beneficial commensals, confirmed the prebiotic value of these xylooligosaccharides.
28490053	5	29	theme	lactic	787:792	arg1	acid					794:797	acetic and lactic acid	776:797	acetic and lactic acid	776:797	The strong production of acetic and lactic acid, the decrease of potentially pathogenic bacteria and the increase of bifidobacteria, and possible beneficial commensals, confirmed the prebiotic value of these xylooligosaccharides.
28490053	1	30	dep	family	168:173	arg1	GH11					179:182	GH11	179:182	GH11	179:182	A fungal endoxylanase belonging to the glycoside hydrolase gene family 11 (GH11) was obtained from the ascomycete Talaromyces amestolkiae.
28490053	1	30	dep	family	168:173	arg1	11					175:176	11	175:176	11	175:176	A fungal endoxylanase belonging to the glycoside hydrolase gene family 11 (GH11) was obtained from the ascomycete Talaromyces amestolkiae.
28490053	2	31	from	xylan	357:361	arg1	xylooligosaccharides					315:334	xylooligosaccharides	315:334	xylooligosaccharides (XOS) from birchwood xylan	315:361	The enzyme was purified, characterized and used to produce a mixture of xylooligosaccharides (XOS) from birchwood xylan.
28490053	2	31	from	xylan	357:361	arg1	mixture					304:310	a mixture	302:310	a mixture of xylooligosaccharides (XOS) from birchwood xylan	302:361	The enzyme was purified, characterized and used to produce a mixture of xylooligosaccharides (XOS) from birchwood xylan.
28490053	2	31	from	xylan	357:361	arg1	XOS					337:339	XOS	337:339	XOS	337:339	The enzyme was purified, characterized and used to produce a mixture of xylooligosaccharides (XOS) from birchwood xylan.
28490053	1	32	theme	fungal	106:111	arg1	endoxylanase					113:124	A fungal endoxylanase	104:124	A fungal endoxylanase belonging to the glycoside hydrolase gene family 11 (GH11)	104:183	A fungal endoxylanase belonging to the glycoside hydrolase gene family 11 (GH11) was obtained from the ascomycete Talaromyces amestolkiae.
28490053	0	33	theme	birchwood	55:63	arg1	xylan					65:69	birchwood xylan	55:69	birchwood xylan	55:69	Prebiotic effect of xylooligosaccharides produced from birchwood xylan by a novel fungal GH11 xylanase.
28490053	2	34	theme	xylooligosaccharides	315:334	arg1	mixture					304:310	a mixture	302:310	a mixture of xylooligosaccharides (XOS) from birchwood xylan	302:361	The enzyme was purified, characterized and used to produce a mixture of xylooligosaccharides (XOS) from birchwood xylan.
28490053	2	35	used	used	286:289	arg2	enzyme					247:252	The enzyme	243:252	The enzyme	243:252	The enzyme was purified, characterized and used to produce a mixture of xylooligosaccharides (XOS) from birchwood xylan.
28490053	5	36	theme	xylooligosaccharides	959:978	arg1	value					944:948	the prebiotic value	930:948	the prebiotic value of these xylooligosaccharides	930:978	The strong production of acetic and lactic acid, the decrease of potentially pathogenic bacteria and the increase of bifidobacteria, and possible beneficial commensals, confirmed the prebiotic value of these xylooligosaccharides.
28490053	3	37	dep	obtained	399:406	arg1	%					413:413	28.8%	409:413	28.8%	409:413	A notable yield of neutral XOS was obtained (28.8%) upon enzyme treatment and the mixture contained a negligible amount of xylose, having xylobiose, xylotriose and xylotetraose as its main components.
28490053	4	38	theme	organic	687:693	arg1	acids					695:699	organic acids	687:699	organic acids	687:699	The prebiotic potential of this mixture was demonstrated upon analyzing the variations in microorganisms' composition and organic acids profile in breast-fed child faeces fermentations.
28490053	4	39	dep	composition	671:681	arg1	profile					701:707	profile	701:707	profile	701:707	The prebiotic potential of this mixture was demonstrated upon analyzing the variations in microorganisms' composition and organic acids profile in breast-fed child faeces fermentations.
28490053	5	40	theme	prebiotic	934:942	arg1	value					944:948	the prebiotic value	930:948	the prebiotic value of these xylooligosaccharides	930:978	The strong production of acetic and lactic acid, the decrease of potentially pathogenic bacteria and the increase of bifidobacteria, and possible beneficial commensals, confirmed the prebiotic value of these xylooligosaccharides.
28490053	5	41	theme	beneficial	897:906	arg1	commensals					908:917	possible beneficial commensals	888:917	possible beneficial commensals	888:917	The strong production of acetic and lactic acid, the decrease of potentially pathogenic bacteria and the increase of bifidobacteria, and possible beneficial commensals, confirmed the prebiotic value of these xylooligosaccharides.
28490053	1	42	theme	ascomycete	207:216	arg1	amestolkiae					230:240	the ascomycete Talaromyces amestolkiae	203:240	the ascomycete Talaromyces amestolkiae	203:240	A fungal endoxylanase belonging to the glycoside hydrolase gene family 11 (GH11) was obtained from the ascomycete Talaromyces amestolkiae.
28490053	4	43	theme	prebiotic	569:577	arg1	potential					579:587	The prebiotic potential	565:587	The prebiotic potential of this mixture	565:603	The prebiotic potential of this mixture was demonstrated upon analyzing the variations in microorganisms' composition and organic acids profile in breast-fed child faeces fermentations.
28490053	2	44	theme	birchwood	347:355	arg1	xylan					357:361	birchwood xylan	347:361	birchwood xylan	347:361	The enzyme was purified, characterized and used to produce a mixture of xylooligosaccharides (XOS) from birchwood xylan.
28490053	1	45	theme	Talaromyces	218:228	arg1	amestolkiae					230:240	the ascomycete Talaromyces amestolkiae	203:240	the ascomycete Talaromyces amestolkiae	203:240	A fungal endoxylanase belonging to the glycoside hydrolase gene family 11 (GH11) was obtained from the ascomycete Talaromyces amestolkiae.
28490053	1	46	theme	glycoside	143:151	arg1	family					168:173	the glycoside hydrolase gene family 11 (GH11)	139:183	the glycoside hydrolase gene family 11 (GH11)	139:183	A fungal endoxylanase belonging to the glycoside hydrolase gene family 11 (GH11) was obtained from the ascomycete Talaromyces amestolkiae.
27049295	0	0	theme	autophagy	94:102	arg1	signalling					104:113	autophagy signalling	94:113	autophagy signalling	94:113	Astragalus polysaccharides decrease muscle wasting through Akt/mTOR, ubiquitin proteasome and autophagy signalling in 5/6 nephrectomised rats.
27049295	7	1	theme	factors	1106:1112	arg1	levels					1051:1056	The levels	1047:1056	The levels of serum pro-inflammatory factors and oxidative factors	1047:1112	The levels of serum pro-inflammatory factors and oxidative factors were restored by APS treatment.
27049295	7	2	theme	APS	1131:1133	arg1	treatment					1135:1143	APS treatment	1131:1143	APS treatment	1131:1143	The levels of serum pro-inflammatory factors and oxidative factors were restored by APS treatment.
27049295	6	3	theme	weight	980:985	arg1	loss					987:990	weight loss	980:990	weight loss	980:990	RESULTS Data in vivo show 2% APS could alleviate weight loss and improve protein metabolism in nephrectomised rats.
27049295	3	4	theme	5/6	499:501	arg1	nephrectomy					503:513	5/6 nephrectomy	499:513	5/6 nephrectomy	499:513	MATERIALS AND METHODS We performed 5/6 nephrectomy or sham operation in 160 6-week-old Sprague-Dawley rats, and feed animals with or without 2% APS for 155 days.
27049295	2	5	from	level	430:434	arg1	rats					458:461	5/6 nephrectomised rats	439:461	5/6 nephrectomised rats	439:461	AIM OF THE STUDY In this study, we aimed to examine the effects of APS on muscle wasting at molecular level in 5/6 nephrectomised rats.
27049295	10	6	theme	potential	1572:1580	arg1	targets					1582:1588	potential targets	1572:1588	potential targets	1572:1588	CONCLUSIONS Our results suggested APS could improve muscle wasting through Akt/mTOR, ubiquitin proteasome and autophagy signalling, and SLC38A2 may be one of potential targets.
27049295	3	7	dep	MATERIALS	464:472	arg1	We					486:487	We	486:487	We	486:487	MATERIALS AND METHODS We performed 5/6 nephrectomy or sham operation in 160 6-week-old Sprague-Dawley rats, and feed animals with or without 2% APS for 155 days.
27049295	4	8	theme	protein	691:697	arg1	metabolism					699:708	protein metabolism	691:708	protein metabolism	691:708	After treatment, we compared the change of weight, muscle fibre, protein metabolism, pro-inflammatory factors (TNF-α, IL-15, CRP) and oxidative factors (MDA, SOD) among each group.
27049295	7	9	theme	oxidative	1096:1104	arg1	factors					1106:1112	oxidative factors	1096:1112	oxidative factors	1096:1112	The levels of serum pro-inflammatory factors and oxidative factors were restored by APS treatment.
27049295	6	10	dep	RESULTS	931:937	arg1	show					952:955	show	952:955	show 2% APS	952:962	RESULTS Data in vivo show 2% APS could alleviate weight loss and improve protein metabolism in nephrectomised rats.
27049295	5	11	theme	autophagy	868:876	arg1	signalling					878:887	autophagy signalling	868:887	autophagy signalling	868:887	In addition, we detected the Akt/mTOR, ubiquitin proteasome, autophagy signalling and AA transporters in vivo and in vitro.
27049295	2	12	theme	STUDY	339:343	arg1	AIM					328:330	AIM	328:330	AIM OF THE STUDY	328:343	AIM OF THE STUDY In this study, we aimed to examine the effects of APS on muscle wasting at molecular level in 5/6 nephrectomised rats.
27049295	0	13	theme	nephrectomised	122:135	arg1	rats					137:140	5/6 nephrectomised rats	118:140	5/6 nephrectomised rats	118:140	Astragalus polysaccharides decrease muscle wasting through Akt/mTOR, ubiquitin proteasome and autophagy signalling in 5/6 nephrectomised rats.
27049295	0	14	from	Akt/mTOR	59:66	arg1	rats					137:140	5/6 nephrectomised rats	118:140	5/6 nephrectomised rats	118:140	Astragalus polysaccharides decrease muscle wasting through Akt/mTOR, ubiquitin proteasome and autophagy signalling in 5/6 nephrectomised rats.
27049295	8	15	theme	molecular	1149:1157	arg1	levels					1159:1164	molecular levels	1149:1164	molecular levels	1149:1164	In molecular levels, APS restored Akt/mTOR, MAFbx, MuRF1, Atg7, LC3B-II/LC3B-I and SLC38A2 which changed in nephrectomised rats.
27049295	3	16	theme	sham	518:521	arg1	operation					523:531	sham operation	518:531	sham operation	518:531	MATERIALS AND METHODS We performed 5/6 nephrectomy or sham operation in 160 6-week-old Sprague-Dawley rats, and feed animals with or without 2% APS for 155 days.
27049295	9	17	theme	APS	1314:1316	arg1	dose					1306:1309	the optimal dose	1294:1309	the optimal dose of APS	1294:1316	Data in vitro show the optimal dose of APS is 0.2mg/mL, and SLC38A2 siRNA attenuated the effects of 0.2mg/mL APS on atrophy and autophagy.
27049295	9	17	theme	APS	1314:1316	arg1	0.2mg/mL					1321:1328	0.2mg/mL	1321:1328	0.2mg/mL	1321:1328	Data in vitro show the optimal dose of APS is 0.2mg/mL, and SLC38A2 siRNA attenuated the effects of 0.2mg/mL APS on atrophy and autophagy.
27049295	1	18	theme	Radix	206:210	arg1	Astragali					212:220	Radix Astragali	206:220	Radix Astragali	206:220	ETHNOPHARMACOLOGICAL RELEVANCE Existing evidences suggest that Radix Astragali and its polysaccharides composition (APS) can improve muscle mass, but the mechanisms need more research.
27049295	7	19	theme	factors	1084:1090	arg1	levels					1051:1056	The levels	1047:1056	The levels of serum pro-inflammatory factors and oxidative factors	1047:1112	The levels of serum pro-inflammatory factors and oxidative factors were restored by APS treatment.
27049295	4	20	theme	factors	770:776	arg1	change					659:664	the change	655:664	the change of weight, muscle fibre, protein metabolism, pro-inflammatory factors (TNF-α, IL-15, CRP) and oxidative factors (MDA, SOD)	655:787	After treatment, we compared the change of weight, muscle fibre, protein metabolism, pro-inflammatory factors (TNF-α, IL-15, CRP) and oxidative factors (MDA, SOD) among each group.
27049295	4	21	theme	factors	728:734	arg1	change					659:664	the change	655:664	the change of weight, muscle fibre, protein metabolism, pro-inflammatory factors (TNF-α, IL-15, CRP) and oxidative factors (MDA, SOD)	655:787	After treatment, we compared the change of weight, muscle fibre, protein metabolism, pro-inflammatory factors (TNF-α, IL-15, CRP) and oxidative factors (MDA, SOD) among each group.
27049295	6	22	theme	%	958:958	arg1	APS					960:962	2% APS	957:962	2% APS	957:962	RESULTS Data in vivo show 2% APS could alleviate weight loss and improve protein metabolism in nephrectomised rats.
27049295	9	23	theme	APS	1384:1386	arg1	effects					1364:1370	the effects	1360:1370	the effects of 0.2mg/mL APS on atrophy and autophagy	1360:1411	Data in vitro show the optimal dose of APS is 0.2mg/mL, and SLC38A2 siRNA attenuated the effects of 0.2mg/mL APS on atrophy and autophagy.
27049295	9	24	from	effects	1364:1370	arg1	autophagy					1403:1411	autophagy	1403:1411	autophagy	1403:1411	Data in vitro show the optimal dose of APS is 0.2mg/mL, and SLC38A2 siRNA attenuated the effects of 0.2mg/mL APS on atrophy and autophagy.
27049295	9	24	from	effects	1364:1370	arg1	atrophy					1391:1397	atrophy	1391:1397	atrophy	1391:1397	Data in vitro show the optimal dose of APS is 0.2mg/mL, and SLC38A2 siRNA attenuated the effects of 0.2mg/mL APS on atrophy and autophagy.
27049295	6	25	theme	2	957:957	arg1	%					958:958	%	958:958	%	958:958	RESULTS Data in vivo show 2% APS could alleviate weight loss and improve protein metabolism in nephrectomised rats.
27049295	0	26	theme	Astragalus	0:9	arg1	polysaccharides					11:25	Astragalus polysaccharides	0:25	Astragalus polysaccharides	0:25	Astragalus polysaccharides decrease muscle wasting through Akt/mTOR, ubiquitin proteasome and autophagy signalling in 5/6 nephrectomised rats.
27049295	4	27	theme	fibre	684:688	arg1	muscle					677:682	muscle fibre	677:688	muscle fibre	677:688	After treatment, we compared the change of weight, muscle fibre, protein metabolism, pro-inflammatory factors (TNF-α, IL-15, CRP) and oxidative factors (MDA, SOD) among each group.
27049295	4	28	theme	pro-inflammatory	711:726	arg1	factors					728:734	pro-inflammatory factors	711:734	pro-inflammatory factors (TNF-α, IL-15, CRP)	711:754	After treatment, we compared the change of weight, muscle fibre, protein metabolism, pro-inflammatory factors (TNF-α, IL-15, CRP) and oxidative factors (MDA, SOD) among each group.
27049295	9	29	theme	0.2mg/mL	1375:1382	arg1	APS					1384:1386	0.2mg/mL APS	1375:1386	0.2mg/mL APS	1375:1386	Data in vitro show the optimal dose of APS is 0.2mg/mL, and SLC38A2 siRNA attenuated the effects of 0.2mg/mL APS on atrophy and autophagy.
27049295	0	30	theme	muscle	36:41	arg1	wasting					43:49	muscle wasting	36:49	muscle wasting	36:49	Astragalus polysaccharides decrease muscle wasting through Akt/mTOR, ubiquitin proteasome and autophagy signalling in 5/6 nephrectomised rats.
27049295	10	31	theme	muscle	1466:1471	arg1	wasting					1473:1479	muscle wasting	1466:1479	muscle wasting	1466:1479	CONCLUSIONS Our results suggested APS could improve muscle wasting through Akt/mTOR, ubiquitin proteasome and autophagy signalling, and SLC38A2 may be one of potential targets.
27049295	3	32	theme	6-week-old	540:549	arg1	rats					566:569	160 6-week-old Sprague-Dawley rats	536:569	160 6-week-old Sprague-Dawley rats	536:569	MATERIALS AND METHODS We performed 5/6 nephrectomy or sham operation in 160 6-week-old Sprague-Dawley rats, and feed animals with or without 2% APS for 155 days.
27049295	1	33	theme	polysaccharides	230:244	arg1	composition					246:256	its polysaccharides composition	226:256	its polysaccharides composition (APS)	226:262	ETHNOPHARMACOLOGICAL RELEVANCE Existing evidences suggest that Radix Astragali and its polysaccharides composition (APS) can improve muscle mass, but the mechanisms need more research.
27049295	1	33	theme	polysaccharides	230:244	arg1	APS					259:261	APS	259:261	APS	259:261	ETHNOPHARMACOLOGICAL RELEVANCE Existing evidences suggest that Radix Astragali and its polysaccharides composition (APS) can improve muscle mass, but the mechanisms need more research.
27049295	4	34	dep	factors	770:776	arg1	MDA					779:781	MDA	779:781	MDA	779:781	After treatment, we compared the change of weight, muscle fibre, protein metabolism, pro-inflammatory factors (TNF-α, IL-15, CRP) and oxidative factors (MDA, SOD) among each group.
27049295	4	34	dep	factors	770:776	arg1	SOD					784:786	SOD	784:786	SOD	784:786	After treatment, we compared the change of weight, muscle fibre, protein metabolism, pro-inflammatory factors (TNF-α, IL-15, CRP) and oxidative factors (MDA, SOD) among each group.
27049295	1	35	theme	more	313:316	arg1	research					318:325	more research	313:325	more research	313:325	ETHNOPHARMACOLOGICAL RELEVANCE Existing evidences suggest that Radix Astragali and its polysaccharides composition (APS) can improve muscle mass, but the mechanisms need more research.
27049295	10	36	dep	CONCLUSIONS	1414:1424	arg1	one					1565:1567	one	1565:1567	one	1565:1567	CONCLUSIONS Our results suggested APS could improve muscle wasting through Akt/mTOR, ubiquitin proteasome and autophagy signalling, and SLC38A2 may be one of potential targets.
27049295	10	36	dep	CONCLUSIONS	1414:1424	arg1	targets					1582:1588	potential targets	1572:1588	potential targets	1572:1588	CONCLUSIONS Our results suggested APS could improve muscle wasting through Akt/mTOR, ubiquitin proteasome and autophagy signalling, and SLC38A2 may be one of potential targets.
27049295	10	36	dep	CONCLUSIONS	1414:1424	arg1	suggested					1438:1446	suggested	1438:1446	suggested APS could improve muscle wasting through Akt/mTOR, ubiquitin proteasome and autophagy signalling	1438:1543	CONCLUSIONS Our results suggested APS could improve muscle wasting through Akt/mTOR, ubiquitin proteasome and autophagy signalling, and SLC38A2 may be one of potential targets.
27049295	3	37	theme	Sprague-Dawley	551:564	arg1	rats					566:569	160 6-week-old Sprague-Dawley rats	536:569	160 6-week-old Sprague-Dawley rats	536:569	MATERIALS AND METHODS We performed 5/6 nephrectomy or sham operation in 160 6-week-old Sprague-Dawley rats, and feed animals with or without 2% APS for 155 days.
27049295	9	38	theme	optimal	1298:1304	arg1	dose					1306:1309	the optimal dose	1294:1309	the optimal dose of APS	1294:1316	Data in vitro show the optimal dose of APS is 0.2mg/mL, and SLC38A2 siRNA attenuated the effects of 0.2mg/mL APS on atrophy and autophagy.
27049295	9	38	theme	optimal	1298:1304	arg1	0.2mg/mL					1321:1328	0.2mg/mL	1321:1328	0.2mg/mL	1321:1328	Data in vitro show the optimal dose of APS is 0.2mg/mL, and SLC38A2 siRNA attenuated the effects of 0.2mg/mL APS on atrophy and autophagy.
27049295	6	39	theme	in	944:945	arg1	Data					939:942	Data	939:942	Data in vivo	939:950	RESULTS Data in vivo show 2% APS could alleviate weight loss and improve protein metabolism in nephrectomised rats.
27049295	10	40	dep	suggested	1438:1446	arg1	improve					1458:1464	improve	1458:1464	suggested APS could improve muscle wasting through Akt/mTOR, ubiquitin proteasome and autophagy signalling	1438:1543	CONCLUSIONS Our results suggested APS could improve muscle wasting through Akt/mTOR, ubiquitin proteasome and autophagy signalling, and SLC38A2 may be one of potential targets.
27049295	7	41	theme	serum	1061:1065	arg1	factors					1084:1090	serum pro-inflammatory factors	1061:1090	serum pro-inflammatory factors	1061:1090	The levels of serum pro-inflammatory factors and oxidative factors were restored by APS treatment.
27049295	10	42	theme	ubiquitin	1499:1507	arg1	proteasome					1509:1518	ubiquitin proteasome	1499:1518	ubiquitin proteasome	1499:1518	CONCLUSIONS Our results suggested APS could improve muscle wasting through Akt/mTOR, ubiquitin proteasome and autophagy signalling, and SLC38A2 may be one of potential targets.
27049295	9	43	dep	in	1280:1281	arg1	vitro					1283:1287	vitro	1283:1287	vitro	1283:1287	Data in vitro show the optimal dose of APS is 0.2mg/mL, and SLC38A2 siRNA attenuated the effects of 0.2mg/mL APS on atrophy and autophagy.
27049295	10	44	theme	autophagy	1524:1532	arg1	signalling					1534:1543	autophagy signalling	1524:1543	autophagy signalling	1524:1543	CONCLUSIONS Our results suggested APS could improve muscle wasting through Akt/mTOR, ubiquitin proteasome and autophagy signalling, and SLC38A2 may be one of potential targets.
27049295	0	45	from	proteasome	79:88	arg1	rats					137:140	5/6 nephrectomised rats	118:140	5/6 nephrectomised rats	118:140	Astragalus polysaccharides decrease muscle wasting through Akt/mTOR, ubiquitin proteasome and autophagy signalling in 5/6 nephrectomised rats.
27049295	2	46	theme	molecular	420:428	arg1	level					430:434	molecular level	420:434	molecular level in 5/6 nephrectomised rats	420:461	AIM OF THE STUDY In this study, we aimed to examine the effects of APS on muscle wasting at molecular level in 5/6 nephrectomised rats.
27049295	2	47	theme	APS	395:397	arg1	effects					384:390	the effects	380:390	the effects of APS on muscle wasting at molecular level in 5/6 nephrectomised rats	380:461	AIM OF THE STUDY In this study, we aimed to examine the effects of APS on muscle wasting at molecular level in 5/6 nephrectomised rats.
27049295	2	48	theme	nephrectomised	443:456	arg1	rats					458:461	5/6 nephrectomised rats	439:461	5/6 nephrectomised rats	439:461	AIM OF THE STUDY In this study, we aimed to examine the effects of APS on muscle wasting at molecular level in 5/6 nephrectomised rats.
27049295	4	49	theme	oxidative	760:768	arg1	factors					770:776	oxidative factors	760:776	oxidative factors (MDA, SOD)	760:787	After treatment, we compared the change of weight, muscle fibre, protein metabolism, pro-inflammatory factors (TNF-α, IL-15, CRP) and oxidative factors (MDA, SOD) among each group.
27049295	4	50	theme	weight	669:674	arg1	change					659:664	the change	655:664	the change of weight, muscle fibre, protein metabolism, pro-inflammatory factors (TNF-α, IL-15, CRP) and oxidative factors (MDA, SOD)	655:787	After treatment, we compared the change of weight, muscle fibre, protein metabolism, pro-inflammatory factors (TNF-α, IL-15, CRP) and oxidative factors (MDA, SOD) among each group.
27049295	5	51	theme	ubiquitin	846:854	arg1	proteasome					856:865	ubiquitin proteasome	846:865	ubiquitin proteasome	846:865	In addition, we detected the Akt/mTOR, ubiquitin proteasome, autophagy signalling and AA transporters in vivo and in vitro.
27049295	6	52	theme	nephrectomised	1026:1039	arg1	rats					1041:1044	nephrectomised rats	1026:1044	nephrectomised rats	1026:1044	RESULTS Data in vivo show 2% APS could alleviate weight loss and improve protein metabolism in nephrectomised rats.
27049295	6	53	from	metabolism	1012:1021	arg1	rats					1041:1044	nephrectomised rats	1026:1044	nephrectomised rats	1026:1044	RESULTS Data in vivo show 2% APS could alleviate weight loss and improve protein metabolism in nephrectomised rats.
27049295	6	54	theme	protein	1004:1010	arg1	metabolism					1012:1021	protein metabolism	1004:1021	protein metabolism in nephrectomised rats	1004:1044	RESULTS Data in vivo show 2% APS could alleviate weight loss and improve protein metabolism in nephrectomised rats.
27049295	5	55	theme	AA	893:894	arg1	transporters					896:907	AA transporters	893:907	AA transporters	893:907	In addition, we detected the Akt/mTOR, ubiquitin proteasome, autophagy signalling and AA transporters in vivo and in vitro.
27049295	3	56	theme	2	605:605	arg1	%					606:606	%	606:606	%	606:606	MATERIALS AND METHODS We performed 5/6 nephrectomy or sham operation in 160 6-week-old Sprague-Dawley rats, and feed animals with or without 2% APS for 155 days.
27049295	2	57	from	effects	384:390	arg1	muscle					402:407	muscle	402:407	muscle wasting at molecular level in 5/6 nephrectomised rats	402:461	AIM OF THE STUDY In this study, we aimed to examine the effects of APS on muscle wasting at molecular level in 5/6 nephrectomised rats.
27049295	1	58	theme	ETHNOPHARMACOLOGICAL	143:162	arg1	evidences					183:191	ETHNOPHARMACOLOGICAL RELEVANCE Existing evidences	143:191	ETHNOPHARMACOLOGICAL RELEVANCE Existing evidences	143:191	ETHNOPHARMACOLOGICAL RELEVANCE Existing evidences suggest that Radix Astragali and its polysaccharides composition (APS) can improve muscle mass, but the mechanisms need more research.
27049295	4	59	theme	metabolism	699:708	arg1	change					659:664	the change	655:664	the change of weight, muscle fibre, protein metabolism, pro-inflammatory factors (TNF-α, IL-15, CRP) and oxidative factors (MDA, SOD)	655:787	After treatment, we compared the change of weight, muscle fibre, protein metabolism, pro-inflammatory factors (TNF-α, IL-15, CRP) and oxidative factors (MDA, SOD) among each group.
27049295	10	60	theme	targets	1582:1588	arg1	one					1565:1567	one	1565:1567	one	1565:1567	CONCLUSIONS Our results suggested APS could improve muscle wasting through Akt/mTOR, ubiquitin proteasome and autophagy signalling, and SLC38A2 may be one of potential targets.
27049295	10	60	theme	targets	1582:1588	arg1	targets					1582:1588	potential targets	1572:1588	potential targets	1572:1588	CONCLUSIONS Our results suggested APS could improve muscle wasting through Akt/mTOR, ubiquitin proteasome and autophagy signalling, and SLC38A2 may be one of potential targets.
27049295	6	61	dep	in	944:945	arg1	vivo					947:950	vivo	947:950	vivo	947:950	RESULTS Data in vivo show 2% APS could alleviate weight loss and improve protein metabolism in nephrectomised rats.
27049295	3	62	theme	%	606:606	arg1	APS					608:610	2% APS	605:610	2% APS	605:610	MATERIALS AND METHODS We performed 5/6 nephrectomy or sham operation in 160 6-week-old Sprague-Dawley rats, and feed animals with or without 2% APS for 155 days.
27049295	1	63	theme	RELEVANCE	164:172	arg1	evidences					183:191	ETHNOPHARMACOLOGICAL RELEVANCE Existing evidences	143:191	ETHNOPHARMACOLOGICAL RELEVANCE Existing evidences	143:191	ETHNOPHARMACOLOGICAL RELEVANCE Existing evidences suggest that Radix Astragali and its polysaccharides composition (APS) can improve muscle mass, but the mechanisms need more research.
27049295	4	64	theme	muscle	677:682	arg1	change					659:664	the change	655:664	the change of weight, muscle fibre, protein metabolism, pro-inflammatory factors (TNF-α, IL-15, CRP) and oxidative factors (MDA, SOD)	655:787	After treatment, we compared the change of weight, muscle fibre, protein metabolism, pro-inflammatory factors (TNF-α, IL-15, CRP) and oxidative factors (MDA, SOD) among each group.
27049295	9	65	theme	SLC38A2	1335:1341	arg1	siRNA					1343:1347	SLC38A2 siRNA	1335:1347	SLC38A2 siRNA	1335:1347	Data in vitro show the optimal dose of APS is 0.2mg/mL, and SLC38A2 siRNA attenuated the effects of 0.2mg/mL APS on atrophy and autophagy.
27049295	0	66	from	signalling	104:113	arg1	rats					137:140	5/6 nephrectomised rats	118:140	5/6 nephrectomised rats	118:140	Astragalus polysaccharides decrease muscle wasting through Akt/mTOR, ubiquitin proteasome and autophagy signalling in 5/6 nephrectomised rats.
27049295	1	67	theme	Existing	174:181	arg1	evidences					183:191	ETHNOPHARMACOLOGICAL RELEVANCE Existing evidences	143:191	ETHNOPHARMACOLOGICAL RELEVANCE Existing evidences	143:191	ETHNOPHARMACOLOGICAL RELEVANCE Existing evidences suggest that Radix Astragali and its polysaccharides composition (APS) can improve muscle mass, but the mechanisms need more research.
27049295	8	68	theme	nephrectomised	1254:1267	arg1	rats					1269:1272	nephrectomised rats	1254:1272	nephrectomised rats	1254:1272	In molecular levels, APS restored Akt/mTOR, MAFbx, MuRF1, Atg7, LC3B-II/LC3B-I and SLC38A2 which changed in nephrectomised rats.
27049295	0	69	theme	ubiquitin	69:77	arg1	proteasome					79:88	ubiquitin proteasome	69:88	ubiquitin proteasome	69:88	Astragalus polysaccharides decrease muscle wasting through Akt/mTOR, ubiquitin proteasome and autophagy signalling in 5/6 nephrectomised rats.
27049295	7	70	theme	pro-inflammatory	1067:1082	arg1	factors					1084:1090	serum pro-inflammatory factors	1061:1090	serum pro-inflammatory factors	1061:1090	The levels of serum pro-inflammatory factors and oxidative factors were restored by APS treatment.
27049295	9	71	theme	in	1280:1281	arg1	Data					1275:1278	Data	1275:1278	Data in vitro	1275:1287	Data in vitro show the optimal dose of APS is 0.2mg/mL, and SLC38A2 siRNA attenuated the effects of 0.2mg/mL APS on atrophy and autophagy.
27049295	4	72	dep	factors	728:734	arg1	IL-15					744:748	IL-15	744:748	IL-15	744:748	After treatment, we compared the change of weight, muscle fibre, protein metabolism, pro-inflammatory factors (TNF-α, IL-15, CRP) and oxidative factors (MDA, SOD) among each group.
27049295	4	72	dep	factors	728:734	arg1	TNF-α					737:741	TNF-α	737:741	TNF-α	737:741	After treatment, we compared the change of weight, muscle fibre, protein metabolism, pro-inflammatory factors (TNF-α, IL-15, CRP) and oxidative factors (MDA, SOD) among each group.
27049295	4	72	dep	factors	728:734	arg1	CRP					751:753	CRP	751:753	CRP	751:753	After treatment, we compared the change of weight, muscle fibre, protein metabolism, pro-inflammatory factors (TNF-α, IL-15, CRP) and oxidative factors (MDA, SOD) among each group.
27049295	1	73	theme	muscle	276:281	arg1	mass					283:286	muscle mass	276:286	muscle mass	276:286	ETHNOPHARMACOLOGICAL RELEVANCE Existing evidences suggest that Radix Astragali and its polysaccharides composition (APS) can improve muscle mass, but the mechanisms need more research.
24554117	7	0	theme	molecular	813:821	arg1	dispersion					823:832	a molecular dispersion	811:832	a molecular dispersion of amorphous CZ in the nanofiber mats	811:870	The DSC and XRPD revealed a molecular dispersion of amorphous CZ in the nanofiber mats.
24554117	5	1	theme	antifungal	678:687	arg1	activity					689:696	antifungal activity	678:696	antifungal activity	678:696	The nanofiber mats was further investigated for drug release, antifungal activity and cytotoxicity.
24554117	3	2	theme	electrospinning	532:546	arg1	process					548:554	the electrospinning process	528:554	the electrospinning process	528:554	The solvent system ethanol: water: benzyl alcohol (EtOH:H2O:BzOH) with a 70:20:10 ratio was optimal for the electrospinning process.
24554117	10	3	theme	CZ	1196:1197	arg1	powder					1199:1204	the CZ powder	1192:1204	the CZ powder	1192:1204	The ability of the CZ-loaded nanofiber mats to kill the Candida depended on the amount of CZ in the mats; moreover, the CZ-loaded nanofibers killed the Candida significantly faster than the CZ powder and lozenges with low cytotoxicity.
24554117	9	4	theme	nanofibers	977:986	arg1	mat					988:990	the nanofibers mat	973:990	the nanofibers mat	973:990	A fast dissolved and released of CZ from the nanofibers mat was achieved.
24554117	4	5	theme	Various	557:563	arg1	CZ					576:577	CZ	576:577	CZ	576:577	Various amounts of CZ were loaded into the nanofiber mats.
24554117	4	5	theme	Various	557:563	arg1	amounts					565:571	Various amounts	557:571	Various amounts of CZ	557:577	Various amounts of CZ were loaded into the nanofiber mats.
24554117	6	6	dep	RESULTS	716:722	arg1	diameters					734:742	The fiber diameters	724:742	RESULTS The fiber diameters in the mats	716:754	RESULTS The fiber diameters in the mats were in the nanometer range.
24554117	11	7	theme	nanofiber	1264:1272	arg1	mats					1274:1277	CZ-loaded nanofiber mats	1254:1277	CZ-loaded nanofiber mats	1254:1277	CONCLUSIONS CZ-loaded nanofiber mats were successfully electrospun.
24554117	8	8	theme	loading	877:883	arg1	capacity					885:892	The loading capacity	873:892	The loading capacity	873:892	The loading capacity increased when CZ content was raised.
24554117	10	9	from	mats	1106:1109	arg1	CZ					1096:1097	CZ	1096:1097	CZ	1096:1097	The ability of the CZ-loaded nanofiber mats to kill the Candida depended on the amount of CZ in the mats; moreover, the CZ-loaded nanofibers killed the Candida significantly faster than the CZ powder and lozenges with low cytotoxicity.
24554117	10	9	from	mats	1106:1109	arg1	amount					1086:1091	the amount	1082:1091	the amount of CZ in the mats	1082:1109	The ability of the CZ-loaded nanofiber mats to kill the Candida depended on the amount of CZ in the mats; moreover, the CZ-loaded nanofibers killed the Candida significantly faster than the CZ powder and lozenges with low cytotoxicity.
24554117	3	10	theme	system	436:441	arg1	optimal					516:522	optimal	516:522	optimal	516:522	The solvent system ethanol: water: benzyl alcohol (EtOH:H2O:BzOH) with a 70:20:10 ratio was optimal for the electrospinning process.
24554117	3	10	theme	system	436:441	arg1	ethanol					443:449	The solvent system ethanol	424:449	The solvent system ethanol: water: benzyl alcohol (EtOH:H2O:BzOH) with a 70:20:10 ratio	424:510	The solvent system ethanol: water: benzyl alcohol (EtOH:H2O:BzOH) with a 70:20:10 ratio was optimal for the electrospinning process.
24554117	11	11	theme	CZ-loaded	1254:1262	arg1	mats					1274:1277	CZ-loaded nanofiber mats	1254:1277	CZ-loaded nanofiber mats	1254:1277	CONCLUSIONS CZ-loaded nanofiber mats were successfully electrospun.
24554117	10	12	theme	low	1224:1226	arg1	cytotoxicity					1228:1239	low cytotoxicity	1224:1239	low cytotoxicity	1224:1239	The ability of the CZ-loaded nanofiber mats to kill the Candida depended on the amount of CZ in the mats; moreover, the CZ-loaded nanofibers killed the Candida significantly faster than the CZ powder and lozenges with low cytotoxicity.
24554117	6	13	from	diameters	734:742	arg1	mats					751:754	the mats	747:754	the mats	747:754	RESULTS The fiber diameters in the mats were in the nanometer range.
24554117	1	14	theme	Polyvinylpyrrolidone/Hydroxypropyl-β-cyclodextrin	179:227	arg1	CZ					152:153	CZ	152:153	CZ	152:153	PURPOSE This study investigates fabrication of clotrimazole (CZ)-composited electrospun Polyvinylpyrrolidone/Hydroxypropyl-β-cyclodextrin (PVP/HPβCD) blended nanofiber mats for oral candidiasis applications.
24554117	1	14	theme	Polyvinylpyrrolidone/Hydroxypropyl-β-cyclodextrin	179:227	arg1	mats					259:262	clotrimazole (CZ)-composited electrospun Polyvinylpyrrolidone/Hydroxypropyl-β-cyclodextrin (PVP/HPβCD) blended nanofiber mats	138:262	clotrimazole (CZ)-composited electrospun Polyvinylpyrrolidone/Hydroxypropyl-β-cyclodextrin (PVP/HPβCD) blended nanofiber mats	138:262	PURPOSE This study investigates fabrication of clotrimazole (CZ)-composited electrospun Polyvinylpyrrolidone/Hydroxypropyl-β-cyclodextrin (PVP/HPβCD) blended nanofiber mats for oral candidiasis applications.
24554117	2	15	contain	containing	340:349	arg2	clotrimazole					351:362	clotrimazole	351:362	clotrimazole	351:362	METHODS PVP/HPβCD blended nanofiber mats containing clotrimazole were electrospun and characterized using SEM, DSC and XRPD.
24554117	2	15	contain	containing	340:349	arg1	mats					335:338	blended nanofiber mats	317:338	blended nanofiber mats containing clotrimazole	317:362	METHODS PVP/HPβCD blended nanofiber mats containing clotrimazole were electrospun and characterized using SEM, DSC and XRPD.
24554117	9	16	theme	CZ	965:966	arg1	fast					934:937	fast	934:937	fast	934:937	A fast dissolved and released of CZ from the nanofibers mat was achieved.
24554117	1	17	theme	clotrimazole	138:149	arg1	CZ					152:153	CZ	152:153	CZ	152:153	PURPOSE This study investigates fabrication of clotrimazole (CZ)-composited electrospun Polyvinylpyrrolidone/Hydroxypropyl-β-cyclodextrin (PVP/HPβCD) blended nanofiber mats for oral candidiasis applications.
24554117	1	17	theme	clotrimazole	138:149	arg1	mats					259:262	clotrimazole (CZ)-composited electrospun Polyvinylpyrrolidone/Hydroxypropyl-β-cyclodextrin (PVP/HPβCD) blended nanofiber mats	138:262	clotrimazole (CZ)-composited electrospun Polyvinylpyrrolidone/Hydroxypropyl-β-cyclodextrin (PVP/HPβCD) blended nanofiber mats	138:262	PURPOSE This study investigates fabrication of clotrimazole (CZ)-composited electrospun Polyvinylpyrrolidone/Hydroxypropyl-β-cyclodextrin (PVP/HPβCD) blended nanofiber mats for oral candidiasis applications.
24554117	1	18	theme	PVP/HPβCD	230:238	arg1	CZ					152:153	CZ	152:153	CZ	152:153	PURPOSE This study investigates fabrication of clotrimazole (CZ)-composited electrospun Polyvinylpyrrolidone/Hydroxypropyl-β-cyclodextrin (PVP/HPβCD) blended nanofiber mats for oral candidiasis applications.
24554117	1	18	theme	PVP/HPβCD	230:238	arg1	mats					259:262	clotrimazole (CZ)-composited electrospun Polyvinylpyrrolidone/Hydroxypropyl-β-cyclodextrin (PVP/HPβCD) blended nanofiber mats	138:262	clotrimazole (CZ)-composited electrospun Polyvinylpyrrolidone/Hydroxypropyl-β-cyclodextrin (PVP/HPβCD) blended nanofiber mats	138:262	PURPOSE This study investigates fabrication of clotrimazole (CZ)-composited electrospun Polyvinylpyrrolidone/Hydroxypropyl-β-cyclodextrin (PVP/HPβCD) blended nanofiber mats for oral candidiasis applications.
24554117	3	19	theme	solvent	428:434	arg1	optimal					516:522	optimal	516:522	optimal	516:522	The solvent system ethanol: water: benzyl alcohol (EtOH:H2O:BzOH) with a 70:20:10 ratio was optimal for the electrospinning process.
24554117	3	19	theme	solvent	428:434	arg1	ethanol					443:449	The solvent system ethanol	424:449	The solvent system ethanol: water: benzyl alcohol (EtOH:H2O:BzOH) with a 70:20:10 ratio	424:510	The solvent system ethanol: water: benzyl alcohol (EtOH:H2O:BzOH) with a 70:20:10 ratio was optimal for the electrospinning process.
24554117	5	20	theme	drug	664:667	arg1	release					669:675	drug release	664:675	drug release	664:675	The nanofiber mats was further investigated for drug release, antifungal activity and cytotoxicity.
24554117	0	21	theme	Fast-acting	0:10	arg1	clotrimazole					12:23	Fast-acting clotrimazole	0:23	Fast-acting clotrimazole	0:23	Fast-acting clotrimazole composited PVP/HPβCD nanofibers for oral candidiasis application.
24554117	8	22	theme	CZ	909:910	arg1	content					912:918	CZ content	909:918	CZ content	909:918	The loading capacity increased when CZ content was raised.
24554117	3	23	dep	EtOH	475:478	arg1	BzOH					484:487	BzOH	484:487	EtOH:H2O:BzOH	475:487	The solvent system ethanol: water: benzyl alcohol (EtOH:H2O:BzOH) with a 70:20:10 ratio was optimal for the electrospinning process.
24554117	3	23	dep	EtOH	475:478	arg1	H2O					480:482	H2O	480:482	EtOH:H2O:BzOH	475:487	The solvent system ethanol: water: benzyl alcohol (EtOH:H2O:BzOH) with a 70:20:10 ratio was optimal for the electrospinning process.
24554117	13	24	from	application	1456:1466	arg1	candidiasis					1476:1486	oral candidiasis	1471:1486	oral candidiasis	1471:1486	Further in vivo studies are needed to investigate for their application in oral candidiasis.
24554117	10	25	theme	CZ	1096:1097	arg1	CZ					1096:1097	CZ	1096:1097	CZ	1096:1097	The ability of the CZ-loaded nanofiber mats to kill the Candida depended on the amount of CZ in the mats; moreover, the CZ-loaded nanofibers killed the Candida significantly faster than the CZ powder and lozenges with low cytotoxicity.
24554117	10	25	theme	CZ	1096:1097	arg1	amount					1086:1091	the amount	1082:1091	the amount of CZ in the mats	1082:1109	The ability of the CZ-loaded nanofiber mats to kill the Candida depended on the amount of CZ in the mats; moreover, the CZ-loaded nanofibers killed the Candida significantly faster than the CZ powder and lozenges with low cytotoxicity.
24554117	1	26	theme	blended	241:247	arg1	CZ					152:153	CZ	152:153	CZ	152:153	PURPOSE This study investigates fabrication of clotrimazole (CZ)-composited electrospun Polyvinylpyrrolidone/Hydroxypropyl-β-cyclodextrin (PVP/HPβCD) blended nanofiber mats for oral candidiasis applications.
24554117	1	26	theme	blended	241:247	arg1	mats					259:262	clotrimazole (CZ)-composited electrospun Polyvinylpyrrolidone/Hydroxypropyl-β-cyclodextrin (PVP/HPβCD) blended nanofiber mats	138:262	clotrimazole (CZ)-composited electrospun Polyvinylpyrrolidone/Hydroxypropyl-β-cyclodextrin (PVP/HPβCD) blended nanofiber mats	138:262	PURPOSE This study investigates fabrication of clotrimazole (CZ)-composited electrospun Polyvinylpyrrolidone/Hydroxypropyl-β-cyclodextrin (PVP/HPβCD) blended nanofiber mats for oral candidiasis applications.
24554117	3	27	theme	benzyl	459:464	arg1	alcohol					466:472	benzyl alcohol	459:472	The solvent system ethanol: water: benzyl alcohol (EtOH:H2O:BzOH) with a 70:20:10 ratio	424:510	The solvent system ethanol: water: benzyl alcohol (EtOH:H2O:BzOH) with a 70:20:10 ratio was optimal for the electrospinning process.
24554117	12	28	theme	CZ	1372:1373	arg1	powder					1375:1380	CZ powder	1372:1380	CZ powder	1372:1380	They exhibited rapid antifungal activity in vitro relative to CZ powder and lozenges.
24554117	6	29	theme	fiber	728:732	arg1	diameters					734:742	The fiber diameters	724:742	RESULTS The fiber diameters in the mats	716:754	RESULTS The fiber diameters in the mats were in the nanometer range.
24554117	1	30	theme	nanofiber	249:257	arg1	CZ					152:153	CZ	152:153	CZ	152:153	PURPOSE This study investigates fabrication of clotrimazole (CZ)-composited electrospun Polyvinylpyrrolidone/Hydroxypropyl-β-cyclodextrin (PVP/HPβCD) blended nanofiber mats for oral candidiasis applications.
24554117	1	30	theme	nanofiber	249:257	arg1	mats					259:262	clotrimazole (CZ)-composited electrospun Polyvinylpyrrolidone/Hydroxypropyl-β-cyclodextrin (PVP/HPβCD) blended nanofiber mats	138:262	clotrimazole (CZ)-composited electrospun Polyvinylpyrrolidone/Hydroxypropyl-β-cyclodextrin (PVP/HPβCD) blended nanofiber mats	138:262	PURPOSE This study investigates fabrication of clotrimazole (CZ)-composited electrospun Polyvinylpyrrolidone/Hydroxypropyl-β-cyclodextrin (PVP/HPβCD) blended nanofiber mats for oral candidiasis applications.
24554117	0	31	theme	PVP/HPβCD	36:44	arg1	nanofibers					46:55	PVP/HPβCD nanofibers	36:55	PVP/HPβCD nanofibers	36:55	Fast-acting clotrimazole composited PVP/HPβCD nanofibers for oral candidiasis application.
24554117	13	32	theme	Further	1396:1402	arg1	studies					1412:1418	Further in vivo studies	1396:1418	Further in vivo studies	1396:1418	Further in vivo studies are needed to investigate for their application in oral candidiasis.
24554117	10	33	with	lozenges	1210:1217	arg1	cytotoxicity					1228:1239	low cytotoxicity	1224:1239	low cytotoxicity	1224:1239	The ability of the CZ-loaded nanofiber mats to kill the Candida depended on the amount of CZ in the mats; moreover, the CZ-loaded nanofibers killed the Candida significantly faster than the CZ powder and lozenges with low cytotoxicity.
24554117	5	34	theme	nanofiber	620:628	arg1	mats					630:633	The nanofiber mats	616:633	The nanofiber mats	616:633	The nanofiber mats was further investigated for drug release, antifungal activity and cytotoxicity.
24554117	7	35	theme	nanofiber	857:865	arg1	mats					867:870	the nanofiber mats	853:870	the nanofiber mats	853:870	The DSC and XRPD revealed a molecular dispersion of amorphous CZ in the nanofiber mats.
24554117	3	36	theme	70:20:10	497:504	arg1	ratio					506:510	a 70:20:10 ratio	495:510	a 70:20:10 ratio	495:510	The solvent system ethanol: water: benzyl alcohol (EtOH:H2O:BzOH) with a 70:20:10 ratio was optimal for the electrospinning process.
24554117	4	37	theme	nanofiber	600:608	arg1	mats					610:613	the nanofiber mats	596:613	the nanofiber mats	596:613	Various amounts of CZ were loaded into the nanofiber mats.
24554117	10	38	theme	nanofiber	1035:1043	arg1	mats					1045:1048	the CZ-loaded nanofiber mats	1021:1048	the CZ-loaded nanofiber mats	1021:1048	The ability of the CZ-loaded nanofiber mats to kill the Candida depended on the amount of CZ in the mats; moreover, the CZ-loaded nanofibers killed the Candida significantly faster than the CZ powder and lozenges with low cytotoxicity.
24554117	7	39	theme	CZ	847:848	arg1	dispersion					823:832	a molecular dispersion	811:832	a molecular dispersion of amorphous CZ in the nanofiber mats	811:870	The DSC and XRPD revealed a molecular dispersion of amorphous CZ in the nanofiber mats.
24554117	13	40	theme	oral	1471:1474	arg1	candidiasis					1476:1486	oral candidiasis	1471:1486	oral candidiasis	1471:1486	Further in vivo studies are needed to investigate for their application in oral candidiasis.
24554117	10	41	theme	CZ-loaded	1025:1033	arg1	mats					1045:1048	the CZ-loaded nanofiber mats	1021:1048	the CZ-loaded nanofiber mats	1021:1048	The ability of the CZ-loaded nanofiber mats to kill the Candida depended on the amount of CZ in the mats; moreover, the CZ-loaded nanofibers killed the Candida significantly faster than the CZ powder and lozenges with low cytotoxicity.
24554117	1	42	theme	oral	268:271	arg1	applications					285:296	oral candidiasis applications	268:296	oral candidiasis applications	268:296	PURPOSE This study investigates fabrication of clotrimazole (CZ)-composited electrospun Polyvinylpyrrolidone/Hydroxypropyl-β-cyclodextrin (PVP/HPβCD) blended nanofiber mats for oral candidiasis applications.
24554117	10	43	from	amount	1086:1091	arg1	mats					1106:1109	the mats	1102:1109	the mats	1102:1109	The ability of the CZ-loaded nanofiber mats to kill the Candida depended on the amount of CZ in the mats; moreover, the CZ-loaded nanofibers killed the Candida significantly faster than the CZ powder and lozenges with low cytotoxicity.
24554117	9	44	dep	fast	934:937	arg1	dissolved					939:947	dissolved	939:947	dissolved	939:947	A fast dissolved and released of CZ from the nanofibers mat was achieved.
24554117	9	44	dep	fast	934:937	arg1	released					953:960	released	953:960	released	953:960	A fast dissolved and released of CZ from the nanofibers mat was achieved.
24554117	1	45	theme	candidiasis	273:283	arg1	applications					285:296	oral candidiasis applications	268:296	oral candidiasis applications	268:296	PURPOSE This study investigates fabrication of clotrimazole (CZ)-composited electrospun Polyvinylpyrrolidone/Hydroxypropyl-β-cyclodextrin (PVP/HPβCD) blended nanofiber mats for oral candidiasis applications.
24554117	0	46	theme	candidiasis	66:76	arg1	application					78:88	oral candidiasis application	61:88	oral candidiasis application	61:88	Fast-acting clotrimazole composited PVP/HPβCD nanofibers for oral candidiasis application.
24554117	7	47	from	dispersion	823:832	arg1	mats					867:870	the nanofiber mats	853:870	the nanofiber mats	853:870	The DSC and XRPD revealed a molecular dispersion of amorphous CZ in the nanofiber mats.
24554117	1	48	dep	PURPOSE	91:97	arg1	investigates					110:121	investigates	110:121	investigates fabrication of clotrimazole (CZ)-composited electrospun Polyvinylpyrrolidone/Hydroxypropyl-β-cyclodextrin (PVP/HPβCD) blended nanofiber mats for oral candidiasis applications	110:296	PURPOSE This study investigates fabrication of clotrimazole (CZ)-composited electrospun Polyvinylpyrrolidone/Hydroxypropyl-β-cyclodextrin (PVP/HPβCD) blended nanofiber mats for oral candidiasis applications.
24554117	0	49	theme	oral	61:64	arg1	application					78:88	oral candidiasis application	61:88	oral candidiasis application	61:88	Fast-acting clotrimazole composited PVP/HPβCD nanofibers for oral candidiasis application.
24554117	3	50	with	ethanol	443:449	arg1	ratio					506:510	a 70:20:10 ratio	495:510	a 70:20:10 ratio	495:510	The solvent system ethanol: water: benzyl alcohol (EtOH:H2O:BzOH) with a 70:20:10 ratio was optimal for the electrospinning process.
24554117	4	51	theme	CZ	576:577	arg1	CZ					576:577	CZ	576:577	CZ	576:577	Various amounts of CZ were loaded into the nanofiber mats.
24554117	4	51	theme	CZ	576:577	arg1	amounts					565:571	Various amounts	557:571	Various amounts of CZ	557:577	Various amounts of CZ were loaded into the nanofiber mats.
24554117	10	52	with	Candida	1158:1164	arg1	cytotoxicity					1228:1239	low cytotoxicity	1224:1239	low cytotoxicity	1224:1239	The ability of the CZ-loaded nanofiber mats to kill the Candida depended on the amount of CZ in the mats; moreover, the CZ-loaded nanofibers killed the Candida significantly faster than the CZ powder and lozenges with low cytotoxicity.
24554117	12	53	theme	rapid	1325:1329	arg1	activity					1342:1349	rapid antifungal activity	1325:1349	rapid antifungal activity	1325:1349	They exhibited rapid antifungal activity in vitro relative to CZ powder and lozenges.
24554117	13	54	theme	in	1404:1405	arg1	studies					1412:1418	Further in vivo studies	1396:1418	Further in vivo studies	1396:1418	Further in vivo studies are needed to investigate for their application in oral candidiasis.
24554117	10	55	theme	faster	1180:1185	arg1	Candida					1158:1164	the Candida	1154:1164	the Candida significantly faster than the CZ powder	1154:1204	The ability of the CZ-loaded nanofiber mats to kill the Candida depended on the amount of CZ in the mats; moreover, the CZ-loaded nanofibers killed the Candida significantly faster than the CZ powder and lozenges with low cytotoxicity.
24554117	3	56	dep	ethanol	443:449	arg1	EtOH					475:478	EtOH	475:478	EtOH:H2O:BzOH	475:487	The solvent system ethanol: water: benzyl alcohol (EtOH:H2O:BzOH) with a 70:20:10 ratio was optimal for the electrospinning process.
24554117	3	56	dep	ethanol	443:449	arg1	water					452:456	water	452:456	The solvent system ethanol: water: benzyl alcohol (EtOH:H2O:BzOH) with a 70:20:10 ratio	424:510	The solvent system ethanol: water: benzyl alcohol (EtOH:H2O:BzOH) with a 70:20:10 ratio was optimal for the electrospinning process.
24554117	3	56	dep	ethanol	443:449	arg1	alcohol					466:472	benzyl alcohol	459:472	The solvent system ethanol: water: benzyl alcohol (EtOH:H2O:BzOH) with a 70:20:10 ratio	424:510	The solvent system ethanol: water: benzyl alcohol (EtOH:H2O:BzOH) with a 70:20:10 ratio was optimal for the electrospinning process.
24554117	11	57	dep	CONCLUSIONS	1242:1252	arg1	electrospun					1297:1307	electrospun	1297:1307	were successfully electrospun	1279:1307	CONCLUSIONS CZ-loaded nanofiber mats were successfully electrospun.
24554117	6	58	theme	nanometer	768:776	arg1	range					778:782	the nanometer range	764:782	the nanometer range	764:782	RESULTS The fiber diameters in the mats were in the nanometer range.
24554117	10	59	theme	mats	1045:1048	arg1	ability					1010:1016	The ability	1006:1016	The ability of the CZ-loaded nanofiber mats to kill the Candida	1006:1068	The ability of the CZ-loaded nanofiber mats to kill the Candida depended on the amount of CZ in the mats; moreover, the CZ-loaded nanofibers killed the Candida significantly faster than the CZ powder and lozenges with low cytotoxicity.
24554117	2	60	theme	nanofiber	325:333	arg1	mats					335:338	blended nanofiber mats	317:338	blended nanofiber mats containing clotrimazole	317:362	METHODS PVP/HPβCD blended nanofiber mats containing clotrimazole were electrospun and characterized using SEM, DSC and XRPD.
24554117	1	61	theme	mats	259:262	arg1	fabrication					123:133	fabrication	123:133	fabrication of clotrimazole (CZ)-composited electrospun Polyvinylpyrrolidone/Hydroxypropyl-β-cyclodextrin (PVP/HPβCD) blended nanofiber mats for oral candidiasis applications	123:296	PURPOSE This study investigates fabrication of clotrimazole (CZ)-composited electrospun Polyvinylpyrrolidone/Hydroxypropyl-β-cyclodextrin (PVP/HPβCD) blended nanofiber mats for oral candidiasis applications.
24554117	1	62	theme	-composited	155:165	arg1	CZ					152:153	CZ	152:153	CZ	152:153	PURPOSE This study investigates fabrication of clotrimazole (CZ)-composited electrospun Polyvinylpyrrolidone/Hydroxypropyl-β-cyclodextrin (PVP/HPβCD) blended nanofiber mats for oral candidiasis applications.
24554117	1	62	theme	-composited	155:165	arg1	mats					259:262	clotrimazole (CZ)-composited electrospun Polyvinylpyrrolidone/Hydroxypropyl-β-cyclodextrin (PVP/HPβCD) blended nanofiber mats	138:262	clotrimazole (CZ)-composited electrospun Polyvinylpyrrolidone/Hydroxypropyl-β-cyclodextrin (PVP/HPβCD) blended nanofiber mats	138:262	PURPOSE This study investigates fabrication of clotrimazole (CZ)-composited electrospun Polyvinylpyrrolidone/Hydroxypropyl-β-cyclodextrin (PVP/HPβCD) blended nanofiber mats for oral candidiasis applications.
24554117	12	63	theme	antifungal	1331:1340	arg1	activity					1342:1349	rapid antifungal activity	1325:1349	rapid antifungal activity	1325:1349	They exhibited rapid antifungal activity in vitro relative to CZ powder and lozenges.
24554117	13	64	dep	in	1404:1405	arg1	vivo					1407:1410	vivo	1407:1410	vivo	1407:1410	Further in vivo studies are needed to investigate for their application in oral candidiasis.
24554117	2	65	theme	blended	317:323	arg1	mats					335:338	blended nanofiber mats	317:338	blended nanofiber mats containing clotrimazole	317:362	METHODS PVP/HPβCD blended nanofiber mats containing clotrimazole were electrospun and characterized using SEM, DSC and XRPD.
24554117	1	66	theme	electrospun	167:177	arg1	CZ					152:153	CZ	152:153	CZ	152:153	PURPOSE This study investigates fabrication of clotrimazole (CZ)-composited electrospun Polyvinylpyrrolidone/Hydroxypropyl-β-cyclodextrin (PVP/HPβCD) blended nanofiber mats for oral candidiasis applications.
24554117	1	66	theme	electrospun	167:177	arg1	mats					259:262	clotrimazole (CZ)-composited electrospun Polyvinylpyrrolidone/Hydroxypropyl-β-cyclodextrin (PVP/HPβCD) blended nanofiber mats	138:262	clotrimazole (CZ)-composited electrospun Polyvinylpyrrolidone/Hydroxypropyl-β-cyclodextrin (PVP/HPβCD) blended nanofiber mats	138:262	PURPOSE This study investigates fabrication of clotrimazole (CZ)-composited electrospun Polyvinylpyrrolidone/Hydroxypropyl-β-cyclodextrin (PVP/HPβCD) blended nanofiber mats for oral candidiasis applications.
24554117	10	67	theme	CZ-loaded	1126:1134	arg1	nanofibers					1136:1145	the CZ-loaded nanofibers	1122:1145	the CZ-loaded nanofibers	1122:1145	The ability of the CZ-loaded nanofiber mats to kill the Candida depended on the amount of CZ in the mats; moreover, the CZ-loaded nanofibers killed the Candida significantly faster than the CZ powder and lozenges with low cytotoxicity.
25042545	0	0	theme	thermoplastic	85:97	arg1	NanoPaPreg					99:108	renewable thermoplastic NanoPaPreg	75:108	Bacterial cellulose nanopaper as reinforcement for polylactide composites: renewable thermoplastic NanoPaPreg.	0:109	Bacterial cellulose nanopaper as reinforcement for polylactide composites: renewable thermoplastic NanoPaPreg.
25042545	1	1	theme	prime	159:163	arg1	candidate					165:173	a prime candidate	157:173	a prime candidate nano-reinforcement for the production of renewable nanocomposites	157:239	Bacterial cellulose (BC) is often regarded as a prime candidate nano-reinforcement for the production of renewable nanocomposites.
25042545	1	1	theme	prime	159:163	arg1	cellulose					121:129	Bacterial cellulose	111:129	Bacterial cellulose (BC)	111:134	Bacterial cellulose (BC) is often regarded as a prime candidate nano-reinforcement for the production of renewable nanocomposites.
25042545	4	2	theme	MPa	687:689	arg1	nanoPaPreg					595:604	high-performance nanoPaPreg	578:604	high-performance nanoPaPreg (vf = 65 vol%) with a tensile modulus and strength of 6.9 ± 0.5 GPa and 125 ± 10 MPa	578:689	It is demon-strated that high-performance nanoPaPreg (vf = 65 vol%) with a tensile modulus and strength of 6.9 ± 0.5 GPa and 125 ± 10 MPa, respectively, can be fabricated.
25042545	4	2	theme	MPa	687:689	arg1	%					618:618	vf = 65 vol%	607:618	vf = 65 vol%	607:618	It is demon-strated that high-performance nanoPaPreg (vf = 65 vol%) with a tensile modulus and strength of 6.9 ± 0.5 GPa and 125 ± 10 MPa, respectively, can be fabricated.
25042545	5	3	theme	BC	883:884	arg1	nanofibers					886:895	the individual BC nanofibers	868:895	the individual BC nanofibers	868:895	It is also shown that the tensile properties of nanoPaPreg are predominantly governed by the mechanical performance of BC nanopaper instead of the individual BC nanofibers, due to difficulties impregnating the dense nanofibrous BC network.
25042545	3	4	theme	nanoPaPreg	525:534	arg1	"					535:535	"nanoPaPreg"	524:535	"nanoPaPreg"	524:535	Here, the manufacturing concept of paper-based laminates is used, i.e., "PaPreg," to produce BC nanopaper reinforced PLLA, which has been called "nanoPaPreg" by the authors.
25042545	3	5	theme	BC	472:473	arg1	PLLA					496:499	BC nanopaper reinforced PLLA	472:499	BC nanopaper reinforced PLLA	472:499	Here, the manufacturing concept of paper-based laminates is used, i.e., "PaPreg," to produce BC nanopaper reinforced PLLA, which has been called "nanoPaPreg" by the authors.
25042545	5	6	theme	mechanical	818:827	arg1	performance					829:839	the mechanical performance	814:839	the mechanical performance of BC nanopaper instead of the individual BC nanofibers, due to difficulties impregnating the dense nanofibrous BC network	814:962	It is also shown that the tensile properties of nanoPaPreg are predominantly governed by the mechanical performance of BC nanopaper instead of the individual BC nanofibers, due to difficulties impregnating the dense nanofibrous BC network.
25042545	2	7	theme	nanocomposites	289:302	arg1	performance					266:276	the mechanical performance	251:276	the mechanical performance of most BC nanocomposites	251:302	However, the mechanical performance of most BC nanocomposites is often inferior compared with commercially available polylactide (PLLA).
25042545	0	8	theme	polylactide	51:61	arg1	composites					63:72	polylactide composites	51:72	polylactide composites	51:72	Bacterial cellulose nanopaper as reinforcement for polylactide composites: renewable thermoplastic NanoPaPreg.
25042545	2	9	theme	BC	286:287	arg1	nanocomposites					289:302	most BC nanocomposites	281:302	most BC nanocomposites	281:302	However, the mechanical performance of most BC nanocomposites is often inferior compared with commercially available polylactide (PLLA).
25042545	1	10	theme	nano-reinforcement	175:192	arg1	candidate					165:173	a prime candidate	157:173	a prime candidate nano-reinforcement for the production of renewable nanocomposites	157:239	Bacterial cellulose (BC) is often regarded as a prime candidate nano-reinforcement for the production of renewable nanocomposites.
25042545	1	10	theme	nano-reinforcement	175:192	arg1	cellulose					121:129	Bacterial cellulose	111:129	Bacterial cellulose (BC)	111:134	Bacterial cellulose (BC) is often regarded as a prime candidate nano-reinforcement for the production of renewable nanocomposites.
25042545	0	11	theme	cellulose	10:18	arg1	nanopaper					20:28	Bacterial cellulose nanopaper	0:28	Bacterial cellulose nanopaper as reinforcement for polylactide composites: renewable thermoplastic NanoPaPreg.	0:109	Bacterial cellulose nanopaper as reinforcement for polylactide composites: renewable thermoplastic NanoPaPreg.
25042545	5	12	theme	nanopaper	847:855	arg1	performance					829:839	the mechanical performance	814:839	the mechanical performance of BC nanopaper instead of the individual BC nanofibers, due to difficulties impregnating the dense nanofibrous BC network	814:962	It is also shown that the tensile properties of nanoPaPreg are predominantly governed by the mechanical performance of BC nanopaper instead of the individual BC nanofibers, due to difficulties impregnating the dense nanofibrous BC network.
25042545	5	13	theme	nanoPaPreg	773:782	arg1	properties					759:768	the tensile properties	747:768	the tensile properties of nanoPaPreg	747:782	It is also shown that the tensile properties of nanoPaPreg are predominantly governed by the mechanical performance of BC nanopaper instead of the individual BC nanofibers, due to difficulties impregnating the dense nanofibrous BC network.
25042545	0	14	theme	Bacterial	0:8	arg1	nanopaper					20:28	Bacterial cellulose nanopaper	0:28	Bacterial cellulose nanopaper as reinforcement for polylactide composites: renewable thermoplastic NanoPaPreg.	0:109	Bacterial cellulose nanopaper as reinforcement for polylactide composites: renewable thermoplastic NanoPaPreg.
25042545	4	15	theme	±	664:664	arg1	GPa					670:672	6.9 ± 0.5 GPa	660:672	6.9 ± 0.5 GPa	660:672	It is demon-strated that high-performance nanoPaPreg (vf = 65 vol%) with a tensile modulus and strength of 6.9 ± 0.5 GPa and 125 ± 10 MPa, respectively, can be fabricated.
25042545	1	16	theme	Bacterial	111:119	arg1	BC					132:133	BC	132:133	BC	132:133	Bacterial cellulose (BC) is often regarded as a prime candidate nano-reinforcement for the production of renewable nanocomposites.
25042545	1	16	theme	Bacterial	111:119	arg1	cellulose					121:129	Bacterial cellulose	111:129	Bacterial cellulose (BC)	111:134	Bacterial cellulose (BC) is often regarded as a prime candidate nano-reinforcement for the production of renewable nanocomposites.
25042545	1	16	theme	Bacterial	111:119	arg1	candidate					165:173	a prime candidate	157:173	a prime candidate nano-reinforcement for the production of renewable nanocomposites	157:239	Bacterial cellulose (BC) is often regarded as a prime candidate nano-reinforcement for the production of renewable nanocomposites.
25042545	4	17	theme	GPa	670:672	arg1	nanoPaPreg					595:604	high-performance nanoPaPreg	578:604	high-performance nanoPaPreg (vf = 65 vol%) with a tensile modulus and strength of 6.9 ± 0.5 GPa and 125 ± 10 MPa	578:689	It is demon-strated that high-performance nanoPaPreg (vf = 65 vol%) with a tensile modulus and strength of 6.9 ± 0.5 GPa and 125 ± 10 MPa, respectively, can be fabricated.
25042545	4	17	theme	GPa	670:672	arg1	%					618:618	vf = 65 vol%	607:618	vf = 65 vol%	607:618	It is demon-strated that high-performance nanoPaPreg (vf = 65 vol%) with a tensile modulus and strength of 6.9 ± 0.5 GPa and 125 ± 10 MPa, respectively, can be fabricated.
25042545	5	18	theme	BC	844:845	arg1	nanopaper					847:855	BC nanopaper	844:855	BC nanopaper	844:855	It is also shown that the tensile properties of nanoPaPreg are predominantly governed by the mechanical performance of BC nanopaper instead of the individual BC nanofibers, due to difficulties impregnating the dense nanofibrous BC network.
25042545	4	19	theme	±	682:682	arg1	MPa					687:689	125 ± 10 MPa	678:689	125 ± 10 MPa	678:689	It is demon-strated that high-performance nanoPaPreg (vf = 65 vol%) with a tensile modulus and strength of 6.9 ± 0.5 GPa and 125 ± 10 MPa, respectively, can be fabricated.
25042545	4	20	theme	=	610:610	arg1	nanoPaPreg					595:604	high-performance nanoPaPreg	578:604	high-performance nanoPaPreg (vf = 65 vol%) with a tensile modulus and strength of 6.9 ± 0.5 GPa and 125 ± 10 MPa	578:689	It is demon-strated that high-performance nanoPaPreg (vf = 65 vol%) with a tensile modulus and strength of 6.9 ± 0.5 GPa and 125 ± 10 MPa, respectively, can be fabricated.
25042545	4	20	theme	=	610:610	arg1	%					618:618	vf = 65 vol%	607:618	vf = 65 vol%	607:618	It is demon-strated that high-performance nanoPaPreg (vf = 65 vol%) with a tensile modulus and strength of 6.9 ± 0.5 GPa and 125 ± 10 MPa, respectively, can be fabricated.
25042545	3	21	theme	manufacturing	389:401	arg1	concept					403:409	the manufacturing concept	385:409	the manufacturing concept of paper-based laminates	385:434	Here, the manufacturing concept of paper-based laminates is used, i.e., "PaPreg," to produce BC nanopaper reinforced PLLA, which has been called "nanoPaPreg" by the authors.
25042545	5	22	theme	nanofibers	886:895	arg1	performance					829:839	the mechanical performance	814:839	the mechanical performance of BC nanopaper instead of the individual BC nanofibers, due to difficulties impregnating the dense nanofibrous BC network	814:962	It is also shown that the tensile properties of nanoPaPreg are predominantly governed by the mechanical performance of BC nanopaper instead of the individual BC nanofibers, due to difficulties impregnating the dense nanofibrous BC network.
25042545	5	23	theme	individual	872:881	arg1	nanofibers					886:895	the individual BC nanofibers	868:895	the individual BC nanofibers	868:895	It is also shown that the tensile properties of nanoPaPreg are predominantly governed by the mechanical performance of BC nanopaper instead of the individual BC nanofibers, due to difficulties impregnating the dense nanofibrous BC network.
25042545	4	24	theme	vol	615:617	arg1	nanoPaPreg					595:604	high-performance nanoPaPreg	578:604	high-performance nanoPaPreg (vf = 65 vol%) with a tensile modulus and strength of 6.9 ± 0.5 GPa and 125 ± 10 MPa	578:689	It is demon-strated that high-performance nanoPaPreg (vf = 65 vol%) with a tensile modulus and strength of 6.9 ± 0.5 GPa and 125 ± 10 MPa, respectively, can be fabricated.
25042545	4	24	theme	vol	615:617	arg1	%					618:618	vf = 65 vol%	607:618	vf = 65 vol%	607:618	It is demon-strated that high-performance nanoPaPreg (vf = 65 vol%) with a tensile modulus and strength of 6.9 ± 0.5 GPa and 125 ± 10 MPa, respectively, can be fabricated.
25042545	1	25	theme	renewable	216:224	arg1	nanocomposites					226:239	renewable nanocomposites	216:239	renewable nanocomposites	216:239	Bacterial cellulose (BC) is often regarded as a prime candidate nano-reinforcement for the production of renewable nanocomposites.
25042545	4	26	with	nanoPaPreg	595:604	arg1	strength					648:655	strength	648:655	strength	648:655	It is demon-strated that high-performance nanoPaPreg (vf = 65 vol%) with a tensile modulus and strength of 6.9 ± 0.5 GPa and 125 ± 10 MPa, respectively, can be fabricated.
25042545	4	26	with	nanoPaPreg	595:604	arg1	modulus					636:642	a tensile modulus	626:642	a tensile modulus	626:642	It is demon-strated that high-performance nanoPaPreg (vf = 65 vol%) with a tensile modulus and strength of 6.9 ± 0.5 GPa and 125 ± 10 MPa, respectively, can be fabricated.
25042545	5	27	theme	tensile	751:757	arg1	properties					759:768	the tensile properties	747:768	the tensile properties of nanoPaPreg	747:782	It is also shown that the tensile properties of nanoPaPreg are predominantly governed by the mechanical performance of BC nanopaper instead of the individual BC nanofibers, due to difficulties impregnating the dense nanofibrous BC network.
25042545	1	28	theme	nanocomposites	226:239	arg1	production					202:211	the production	198:211	the production of renewable nanocomposites	198:239	Bacterial cellulose (BC) is often regarded as a prime candidate nano-reinforcement for the production of renewable nanocomposites.
25042545	3	29	theme	paper-based	414:424	arg1	laminates					426:434	paper-based laminates	414:434	paper-based laminates	414:434	Here, the manufacturing concept of paper-based laminates is used, i.e., "PaPreg," to produce BC nanopaper reinforced PLLA, which has been called "nanoPaPreg" by the authors.
25042545	5	30	theme	nanofibrous	941:951	arg1	network					956:962	the dense nanofibrous BC network	931:962	the dense nanofibrous BC network	931:962	It is also shown that the tensile properties of nanoPaPreg are predominantly governed by the mechanical performance of BC nanopaper instead of the individual BC nanofibers, due to difficulties impregnating the dense nanofibrous BC network.
25042545	3	31	theme	nanopaper	475:483	arg1	PLLA					496:499	BC nanopaper reinforced PLLA	472:499	BC nanopaper reinforced PLLA	472:499	Here, the manufacturing concept of paper-based laminates is used, i.e., "PaPreg," to produce BC nanopaper reinforced PLLA, which has been called "nanoPaPreg" by the authors.
25042545	3	32	theme	laminates	426:434	arg1	concept					403:409	the manufacturing concept	385:409	the manufacturing concept of paper-based laminates	385:434	Here, the manufacturing concept of paper-based laminates is used, i.e., "PaPreg," to produce BC nanopaper reinforced PLLA, which has been called "nanoPaPreg" by the authors.
25042545	5	33	theme	BC	953:954	arg1	network					956:962	the dense nanofibrous BC network	931:962	the dense nanofibrous BC network	931:962	It is also shown that the tensile properties of nanoPaPreg are predominantly governed by the mechanical performance of BC nanopaper instead of the individual BC nanofibers, due to difficulties impregnating the dense nanofibrous BC network.
25042545	3	34	theme	reinforced	485:494	arg1	PLLA					496:499	BC nanopaper reinforced PLLA	472:499	BC nanopaper reinforced PLLA	472:499	Here, the manufacturing concept of paper-based laminates is used, i.e., "PaPreg," to produce BC nanopaper reinforced PLLA, which has been called "nanoPaPreg" by the authors.
25042545	3	35	used	used	439:442	arg2	concept					403:409	the manufacturing concept	385:409	the manufacturing concept of paper-based laminates	385:434	Here, the manufacturing concept of paper-based laminates is used, i.e., "PaPreg," to produce BC nanopaper reinforced PLLA, which has been called "nanoPaPreg" by the authors.
25042545	5	36	theme	due	898:900	arg1	nanofibers					886:895	the individual BC nanofibers	868:895	the individual BC nanofibers	868:895	It is also shown that the tensile properties of nanoPaPreg are predominantly governed by the mechanical performance of BC nanopaper instead of the individual BC nanofibers, due to difficulties impregnating the dense nanofibrous BC network.
25042545	2	37	theme	most	281:284	arg1	nanocomposites					289:302	most BC nanocomposites	281:302	most BC nanocomposites	281:302	However, the mechanical performance of most BC nanocomposites is often inferior compared with commercially available polylactide (PLLA).
25042545	5	38	theme	dense	935:939	arg1	network					956:962	the dense nanofibrous BC network	931:962	the dense nanofibrous BC network	931:962	It is also shown that the tensile properties of nanoPaPreg are predominantly governed by the mechanical performance of BC nanopaper instead of the individual BC nanofibers, due to difficulties impregnating the dense nanofibrous BC network.
25042545	4	39	theme	high-performance	578:593	arg1	nanoPaPreg					595:604	high-performance nanoPaPreg	578:604	high-performance nanoPaPreg (vf = 65 vol%) with a tensile modulus and strength of 6.9 ± 0.5 GPa and 125 ± 10 MPa	578:689	It is demon-strated that high-performance nanoPaPreg (vf = 65 vol%) with a tensile modulus and strength of 6.9 ± 0.5 GPa and 125 ± 10 MPa, respectively, can be fabricated.
25042545	4	39	theme	high-performance	578:593	arg1	%					618:618	vf = 65 vol%	607:618	vf = 65 vol%	607:618	It is demon-strated that high-performance nanoPaPreg (vf = 65 vol%) with a tensile modulus and strength of 6.9 ± 0.5 GPa and 125 ± 10 MPa, respectively, can be fabricated.
25042545	0	40	dep	nanopaper	20:28	arg1	NanoPaPreg					99:108	renewable thermoplastic NanoPaPreg	75:108	Bacterial cellulose nanopaper as reinforcement for polylactide composites: renewable thermoplastic NanoPaPreg.	0:109	Bacterial cellulose nanopaper as reinforcement for polylactide composites: renewable thermoplastic NanoPaPreg.
25042545	2	41	theme	available	349:357	arg1	PLLA					372:375	PLLA	372:375	PLLA	372:375	However, the mechanical performance of most BC nanocomposites is often inferior compared with commercially available polylactide (PLLA).
25042545	2	41	theme	available	349:357	arg1	polylactide					359:369	commercially available polylactide	336:369	commercially available polylactide (PLLA)	336:376	However, the mechanical performance of most BC nanocomposites is often inferior compared with commercially available polylactide (PLLA).
25042545	0	42	theme	renewable	75:83	arg1	NanoPaPreg					99:108	renewable thermoplastic NanoPaPreg	75:108	Bacterial cellulose nanopaper as reinforcement for polylactide composites: renewable thermoplastic NanoPaPreg.	0:109	Bacterial cellulose nanopaper as reinforcement for polylactide composites: renewable thermoplastic NanoPaPreg.
25042545	4	43	theme	65	612:613	arg1	vol					615:617	vol	615:617	vol	615:617	It is demon-strated that high-performance nanoPaPreg (vf = 65 vol%) with a tensile modulus and strength of 6.9 ± 0.5 GPa and 125 ± 10 MPa, respectively, can be fabricated.
25042545	2	44	theme	mechanical	255:264	arg1	performance					266:276	the mechanical performance	251:276	the mechanical performance of most BC nanocomposites	251:302	However, the mechanical performance of most BC nanocomposites is often inferior compared with commercially available polylactide (PLLA).
25042545	4	45	theme	tensile	628:634	arg1	modulus					636:642	a tensile modulus	626:642	a tensile modulus	626:642	It is demon-strated that high-performance nanoPaPreg (vf = 65 vol%) with a tensile modulus and strength of 6.9 ± 0.5 GPa and 125 ± 10 MPa, respectively, can be fabricated.
27667544	0	0	from	domain	19:24	arg1	harveyi					59:65	Vibrio harveyi	52:65	Vibrio harveyi	52:65	The chitin-binding domain of a GH-18 chitinase from Vibrio harveyi is crucial for chitin-chitinase interactions.
27667544	8	1	theme	physiological	1395:1407	arg1	role					1409:1412	the physiological role	1391:1412	the physiological role of the chitin-binding domain of the marine bacterial GH-18 chitinase A in chitin-chitinase interactions	1391:1516	These data confirm the physiological role of the chitin-binding domain of the marine bacterial GH-18 chitinase A in chitin-chitinase interactions.
27667544	8	2	theme	chitin-chitinase	1488:1503	arg1	interactions					1505:1516	chitin-chitinase interactions	1488:1516	chitin-chitinase interactions	1488:1516	These data confirm the physiological role of the chitin-binding domain of the marine bacterial GH-18 chitinase A in chitin-chitinase interactions.
27667544	5	3	theme	β-colloidal	924:934	arg1	chitin					936:941	β-colloidal chitin	924:941	β-colloidal chitin	924:941	Chitin binding assays showed that ChBDVhChiA bound to both α- and β-chitins, with the greatest affinity for β-colloidal chitin, but barely bound to polymeric chitosan.
27667544	7	4	theme	polymeric	1318:1326	arg1	substrate					1328:1336	the polymeric substrate	1314:1336	the polymeric substrate into the substrate binding cleft	1314:1369	The binding affinity of the isolated domain was significantly lower than that of intact VhChiA, suggesting that the catalytic domain works synergistically with the chitin-binding domain to guide the polymeric substrate into the substrate binding cleft.
27667544	1	5	theme	TIM	287:289	arg1	domain					308:313	ii) the (α/β)8 TIM barrel catalytic domain	272:313	ii) the (α/β)8 TIM barrel catalytic domain	272:313	Vibrio harveyi chitinase A (VhChiA) is a GH-18 glycosyl hydrolase with a structure containing three distinct domains: i) the N-terminal chitin-binding domain; ii) the (α/β)8 TIM barrel catalytic domain; and iii) the α+β insertion domain.
27667544	4	6	theme	functional	787:796	arg1	characterization					798:813	functional characterization	787:813	functional characterization	787:813	CD measurements suggested that ChBDVhChiA contained β-sheets as major structural components and fluorescence spectroscopy showed that the protein domain was folded correctly, and suitable for functional characterization.
27667544	3	7	theme	pLacI	548:552	arg1	cells					559:563	E. coli BL21 strain Tuner(DE3)pLacI host cells	518:563	E. coli BL21 strain Tuner(DE3)pLacI host cells	518:563	The recombinant ChBDVhChiA was heterologously expressed in E. coli BL21 strain Tuner(DE3)pLacI host cells, and purified to homogeneity.
27667544	8	8	theme	chitinase	1473:1481	arg1	A					1483:1483	the marine bacterial GH-18 chitinase A	1446:1483	the marine bacterial GH-18 chitinase A	1446:1483	These data confirm the physiological role of the chitin-binding domain of the marine bacterial GH-18 chitinase A in chitin-chitinase interactions.
27667544	0	9	theme	chitin-chitinase	82:97	arg1	interactions					99:110	chitin-chitinase interactions	82:110	chitin-chitinase interactions	82:110	The chitin-binding domain of a GH-18 chitinase from Vibrio harveyi is crucial for chitin-chitinase interactions.
27667544	1	10	theme	barrel	291:296	arg1	domain					308:313	ii) the (α/β)8 TIM barrel catalytic domain	272:313	ii) the (α/β)8 TIM barrel catalytic domain	272:313	Vibrio harveyi chitinase A (VhChiA) is a GH-18 glycosyl hydrolase with a structure containing three distinct domains: i) the N-terminal chitin-binding domain; ii) the (α/β)8 TIM barrel catalytic domain; and iii) the α+β insertion domain.
27667544	4	11	theme	major	659:663	arg1	components					676:685	major structural components	659:685	major structural components	659:685	CD measurements suggested that ChBDVhChiA contained β-sheets as major structural components and fluorescence spectroscopy showed that the protein domain was folded correctly, and suitable for functional characterization.
27667544	7	12	theme	domain	1156:1161	arg1	lower					1181:1185	lower	1181:1185	lower	1181:1185	The binding affinity of the isolated domain was significantly lower than that of intact VhChiA, suggesting that the catalytic domain works synergistically with the chitin-binding domain to guide the polymeric substrate into the substrate binding cleft.
27667544	7	12	theme	domain	1156:1161	arg1	affinity					1131:1138	The binding affinity	1119:1138	The binding affinity of the isolated domain	1119:1161	The binding affinity of the isolated domain was significantly lower than that of intact VhChiA, suggesting that the catalytic domain works synergistically with the chitin-binding domain to guide the polymeric substrate into the substrate binding cleft.
27667544	1	13	theme	catalytic	298:306	arg1	domain					308:313	ii) the (α/β)8 TIM barrel catalytic domain	272:313	ii) the (α/β)8 TIM barrel catalytic domain	272:313	Vibrio harveyi chitinase A (VhChiA) is a GH-18 glycosyl hydrolase with a structure containing three distinct domains: i) the N-terminal chitin-binding domain; ii) the (α/β)8 TIM barrel catalytic domain; and iii) the α+β insertion domain.
27667544	3	14	theme	recombinant	463:473	arg1	ChBDVhChiA					475:484	The recombinant ChBDVhChiA	459:484	The recombinant ChBDVhChiA	459:484	The recombinant ChBDVhChiA was heterologously expressed in E. coli BL21 strain Tuner(DE3)pLacI host cells, and purified to homogeneity.
27667544	1	15	theme	N-terminal	238:247	arg1	domain					264:269	the N-terminal chitin-binding domain	234:269	i) the N-terminal chitin-binding domain	231:269	Vibrio harveyi chitinase A (VhChiA) is a GH-18 glycosyl hydrolase with a structure containing three distinct domains: i) the N-terminal chitin-binding domain; ii) the (α/β)8 TIM barrel catalytic domain; and iii) the α+β insertion domain.
27667544	2	16	theme	VhChiA	432:437	arg1	VhChiA					432:437	VhChiA	432:437	VhChiA	432:437	In this study, we cloned the gene fragment encoding the chitin-binding domain of VhChiA, termed ChBDVhChiA.
27667544	2	16	theme	VhChiA	432:437	arg1	domain					422:427	the chitin-binding domain	403:427	the chitin-binding domain	403:427	In this study, we cloned the gene fragment encoding the chitin-binding domain of VhChiA, termed ChBDVhChiA.
27667544	4	17	contain	contained	637:645	arg2	β-sheets					647:654	β-sheets	647:654	β-sheets as major structural components	647:685	CD measurements suggested that ChBDVhChiA contained β-sheets as major structural components and fluorescence spectroscopy showed that the protein domain was folded correctly, and suitable for functional characterization.
27667544	4	17	contain	contained	637:645	arg1	ChBDVhChiA					626:635	ChBDVhChiA	626:635	ChBDVhChiA	626:635	CD measurements suggested that ChBDVhChiA contained β-sheets as major structural components and fluorescence spectroscopy showed that the protein domain was folded correctly, and suitable for functional characterization.
27667544	5	18	theme	greatest	902:909	arg1	affinity					911:918	the greatest affinity	898:918	the greatest affinity for β-colloidal chitin	898:941	Chitin binding assays showed that ChBDVhChiA bound to both α- and β-chitins, with the greatest affinity for β-colloidal chitin, but barely bound to polymeric chitosan.
27667544	6	19	theme	tandem	1013:1018	arg1	sites					1079:1083	the specific sites	1066:1083	the specific sites of enzyme-substrate interactions	1066:1116	These results identified the tandem N-acetamido functionality on chitin chains as the specific sites of enzyme-substrate interactions.
27667544	6	19	theme	tandem	1013:1018	arg1	interactions					1105:1116	enzyme-substrate interactions	1088:1116	enzyme-substrate interactions	1088:1116	These results identified the tandem N-acetamido functionality on chitin chains as the specific sites of enzyme-substrate interactions.
27667544	6	19	theme	tandem	1013:1018	arg1	functionality					1032:1044	the tandem N-acetamido functionality	1009:1044	the tandem N-acetamido functionality on chitin chains	1009:1061	These results identified the tandem N-acetamido functionality on chitin chains as the specific sites of enzyme-substrate interactions.
27667544	1	20	dep	domain	343:348	arg1	iii					320:322	iii	320:322	iii	320:322	Vibrio harveyi chitinase A (VhChiA) is a GH-18 glycosyl hydrolase with a structure containing three distinct domains: i) the N-terminal chitin-binding domain; ii) the (α/β)8 TIM barrel catalytic domain; and iii) the α+β insertion domain.
27667544	4	21	theme	fluorescence	691:702	arg1	spectroscopy					704:715	fluorescence spectroscopy	691:715	fluorescence spectroscopy	691:715	CD measurements suggested that ChBDVhChiA contained β-sheets as major structural components and fluorescence spectroscopy showed that the protein domain was folded correctly, and suitable for functional characterization.
27667544	6	22	theme	N-acetamido	1020:1030	arg1	sites					1079:1083	the specific sites	1066:1083	the specific sites of enzyme-substrate interactions	1066:1116	These results identified the tandem N-acetamido functionality on chitin chains as the specific sites of enzyme-substrate interactions.
27667544	6	22	theme	N-acetamido	1020:1030	arg1	interactions					1105:1116	enzyme-substrate interactions	1088:1116	enzyme-substrate interactions	1088:1116	These results identified the tandem N-acetamido functionality on chitin chains as the specific sites of enzyme-substrate interactions.
27667544	6	22	theme	N-acetamido	1020:1030	arg1	functionality					1032:1044	the tandem N-acetamido functionality	1009:1044	the tandem N-acetamido functionality on chitin chains	1009:1061	These results identified the tandem N-acetamido functionality on chitin chains as the specific sites of enzyme-substrate interactions.
27667544	1	23	theme	GH-18	154:158	arg1	hydrolase					169:177	a GH-18 glycosyl hydrolase	152:177	a GH-18 glycosyl hydrolase with a structure containing three distinct domains: i) the N-terminal chitin-binding domain; ii) the (α/β)8 TIM barrel catalytic domain; and iii) the α+β insertion domain	152:348	Vibrio harveyi chitinase A (VhChiA) is a GH-18 glycosyl hydrolase with a structure containing three distinct domains: i) the N-terminal chitin-binding domain; ii) the (α/β)8 TIM barrel catalytic domain; and iii) the α+β insertion domain.
27667544	1	23	theme	GH-18	154:158	arg1	A					138:138	Vibrio harveyi chitinase A	113:138	Vibrio harveyi chitinase A (VhChiA)	113:147	Vibrio harveyi chitinase A (VhChiA) is a GH-18 glycosyl hydrolase with a structure containing three distinct domains: i) the N-terminal chitin-binding domain; ii) the (α/β)8 TIM barrel catalytic domain; and iii) the α+β insertion domain.
27667544	0	24	theme	chitin-binding	4:17	arg1	domain					19:24	The chitin-binding domain	0:24	The chitin-binding domain of a GH-18 chitinase from Vibrio harveyi	0:65	The chitin-binding domain of a GH-18 chitinase from Vibrio harveyi is crucial for chitin-chitinase interactions.
27667544	0	24	theme	chitin-binding	4:17	arg1	crucial					70:76	crucial	70:76	crucial	70:76	The chitin-binding domain of a GH-18 chitinase from Vibrio harveyi is crucial for chitin-chitinase interactions.
27667544	0	24	theme	chitin-binding	4:17	arg1	chitinase					37:45	a GH-18 chitinase	29:45	a GH-18 chitinase from Vibrio harveyi	29:65	The chitin-binding domain of a GH-18 chitinase from Vibrio harveyi is crucial for chitin-chitinase interactions.
27667544	8	25	from	role	1409:1412	arg1	interactions					1505:1516	chitin-chitinase interactions	1488:1516	chitin-chitinase interactions	1488:1516	These data confirm the physiological role of the chitin-binding domain of the marine bacterial GH-18 chitinase A in chitin-chitinase interactions.
27667544	6	26	theme	interactions	1105:1116	arg1	sites					1079:1083	the specific sites	1066:1083	the specific sites of enzyme-substrate interactions	1066:1116	These results identified the tandem N-acetamido functionality on chitin chains as the specific sites of enzyme-substrate interactions.
27667544	6	26	theme	interactions	1105:1116	arg1	interactions					1105:1116	enzyme-substrate interactions	1088:1116	enzyme-substrate interactions	1088:1116	These results identified the tandem N-acetamido functionality on chitin chains as the specific sites of enzyme-substrate interactions.
27667544	6	26	theme	interactions	1105:1116	arg1	functionality					1032:1044	the tandem N-acetamido functionality	1009:1044	the tandem N-acetamido functionality on chitin chains	1009:1061	These results identified the tandem N-acetamido functionality on chitin chains as the specific sites of enzyme-substrate interactions.
27667544	1	27	theme	glycosyl	160:167	arg1	hydrolase					169:177	a GH-18 glycosyl hydrolase	152:177	a GH-18 glycosyl hydrolase with a structure containing three distinct domains: i) the N-terminal chitin-binding domain; ii) the (α/β)8 TIM barrel catalytic domain; and iii) the α+β insertion domain	152:348	Vibrio harveyi chitinase A (VhChiA) is a GH-18 glycosyl hydrolase with a structure containing three distinct domains: i) the N-terminal chitin-binding domain; ii) the (α/β)8 TIM barrel catalytic domain; and iii) the α+β insertion domain.
27667544	1	27	theme	glycosyl	160:167	arg1	A					138:138	Vibrio harveyi chitinase A	113:138	Vibrio harveyi chitinase A (VhChiA)	113:147	Vibrio harveyi chitinase A (VhChiA) is a GH-18 glycosyl hydrolase with a structure containing three distinct domains: i) the N-terminal chitin-binding domain; ii) the (α/β)8 TIM barrel catalytic domain; and iii) the α+β insertion domain.
27667544	8	28	theme	A	1483:1483	arg1	A					1483:1483	the marine bacterial GH-18 chitinase A	1446:1483	the marine bacterial GH-18 chitinase A	1446:1483	These data confirm the physiological role of the chitin-binding domain of the marine bacterial GH-18 chitinase A in chitin-chitinase interactions.
27667544	8	28	theme	A	1483:1483	arg1	domain					1436:1441	the chitin-binding domain	1417:1441	the chitin-binding domain of the marine bacterial GH-18 chitinase A	1417:1483	These data confirm the physiological role of the chitin-binding domain of the marine bacterial GH-18 chitinase A in chitin-chitinase interactions.
27667544	1	29	theme	chitin-binding	249:262	arg1	domain					264:269	the N-terminal chitin-binding domain	234:269	i) the N-terminal chitin-binding domain	231:269	Vibrio harveyi chitinase A (VhChiA) is a GH-18 glycosyl hydrolase with a structure containing three distinct domains: i) the N-terminal chitin-binding domain; ii) the (α/β)8 TIM barrel catalytic domain; and iii) the α+β insertion domain.
27667544	1	30	contain	containing	196:205	arg2	domain					308:313	ii) the (α/β)8 TIM barrel catalytic domain	272:313	ii) the (α/β)8 TIM barrel catalytic domain	272:313	Vibrio harveyi chitinase A (VhChiA) is a GH-18 glycosyl hydrolase with a structure containing three distinct domains: i) the N-terminal chitin-binding domain; ii) the (α/β)8 TIM barrel catalytic domain; and iii) the α+β insertion domain.
27667544	1	30	contain	containing	196:205	arg1	structure					186:194	a structure	184:194	a structure containing three distinct domains: i) the N-terminal chitin-binding domain; ii) the (α/β)8 TIM barrel catalytic domain; and iii) the α+β insertion domain	184:348	Vibrio harveyi chitinase A (VhChiA) is a GH-18 glycosyl hydrolase with a structure containing three distinct domains: i) the N-terminal chitin-binding domain; ii) the (α/β)8 TIM barrel catalytic domain; and iii) the α+β insertion domain.
27667544	1	30	contain	containing	196:205	arg2	domain					343:348	the α+β insertion domain	325:348	iii) the α+β insertion domain	320:348	Vibrio harveyi chitinase A (VhChiA) is a GH-18 glycosyl hydrolase with a structure containing three distinct domains: i) the N-terminal chitin-binding domain; ii) the (α/β)8 TIM barrel catalytic domain; and iii) the α+β insertion domain.
27667544	1	30	contain	containing	196:205	arg2	domain					264:269	the N-terminal chitin-binding domain	234:269	i) the N-terminal chitin-binding domain	231:269	Vibrio harveyi chitinase A (VhChiA) is a GH-18 glycosyl hydrolase with a structure containing three distinct domains: i) the N-terminal chitin-binding domain; ii) the (α/β)8 TIM barrel catalytic domain; and iii) the α+β insertion domain.
27667544	1	30	contain	containing	196:205	arg2	domains					222:228	three distinct domains	207:228	three distinct domains	207:228	Vibrio harveyi chitinase A (VhChiA) is a GH-18 glycosyl hydrolase with a structure containing three distinct domains: i) the N-terminal chitin-binding domain; ii) the (α/β)8 TIM barrel catalytic domain; and iii) the α+β insertion domain.
27667544	5	31	theme	Chitin	816:821	arg1	assays					831:836	Chitin binding assays	816:836	Chitin binding assays	816:836	Chitin binding assays showed that ChBDVhChiA bound to both α- and β-chitins, with the greatest affinity for β-colloidal chitin, but barely bound to polymeric chitosan.
27667544	4	32	theme	protein	733:739	arg1	domain					741:746	the protein domain	729:746	the protein domain	729:746	CD measurements suggested that ChBDVhChiA contained β-sheets as major structural components and fluorescence spectroscopy showed that the protein domain was folded correctly, and suitable for functional characterization.
27667544	4	33	theme	structural	665:674	arg1	components					676:685	major structural components	659:685	major structural components	659:685	CD measurements suggested that ChBDVhChiA contained β-sheets as major structural components and fluorescence spectroscopy showed that the protein domain was folded correctly, and suitable for functional characterization.
27667544	6	34	theme	enzyme-substrate	1088:1103	arg1	interactions					1105:1116	enzyme-substrate interactions	1088:1116	enzyme-substrate interactions	1088:1116	These results identified the tandem N-acetamido functionality on chitin chains as the specific sites of enzyme-substrate interactions.
27667544	3	35	theme	host	554:557	arg1	cells					559:563	E. coli BL21 strain Tuner(DE3)pLacI host cells	518:563	E. coli BL21 strain Tuner(DE3)pLacI host cells	518:563	The recombinant ChBDVhChiA was heterologously expressed in E. coli BL21 strain Tuner(DE3)pLacI host cells, and purified to homogeneity.
27667544	4	36	theme	CD	595:596	arg1	measurements					598:609	CD measurements	595:609	CD measurements	595:609	CD measurements suggested that ChBDVhChiA contained β-sheets as major structural components and fluorescence spectroscopy showed that the protein domain was folded correctly, and suitable for functional characterization.
27667544	6	37	theme	chitin	1049:1054	arg1	chains					1056:1061	chitin chains	1049:1061	chitin chains	1049:1061	These results identified the tandem N-acetamido functionality on chitin chains as the specific sites of enzyme-substrate interactions.
27667544	0	38	theme	GH-18	31:35	arg1	chitinase					37:45	a GH-18 chitinase	29:45	a GH-18 chitinase from Vibrio harveyi	29:65	The chitin-binding domain of a GH-18 chitinase from Vibrio harveyi is crucial for chitin-chitinase interactions.
27667544	7	39	theme	intact	1200:1205	arg1	VhChiA					1207:1212	intact VhChiA	1200:1212	intact VhChiA	1200:1212	The binding affinity of the isolated domain was significantly lower than that of intact VhChiA, suggesting that the catalytic domain works synergistically with the chitin-binding domain to guide the polymeric substrate into the substrate binding cleft.
27667544	5	40	theme	polymeric	964:972	arg1	chitosan					974:981	polymeric chitosan	964:981	polymeric chitosan	964:981	Chitin binding assays showed that ChBDVhChiA bound to both α- and β-chitins, with the greatest affinity for β-colloidal chitin, but barely bound to polymeric chitosan.
27667544	7	41	theme	catalytic	1235:1243	arg1	domain					1245:1250	the catalytic domain	1231:1250	the catalytic domain	1231:1250	The binding affinity of the isolated domain was significantly lower than that of intact VhChiA, suggesting that the catalytic domain works synergistically with the chitin-binding domain to guide the polymeric substrate into the substrate binding cleft.
27667544	2	42	theme	gene	380:383	arg1	fragment					385:392	the gene fragment	376:392	the gene fragment encoding the chitin-binding domain of VhChiA, termed ChBDVhChiA	376:456	In this study, we cloned the gene fragment encoding the chitin-binding domain of VhChiA, termed ChBDVhChiA.
27667544	0	43	from	harveyi	59:65	arg1	domain					19:24	The chitin-binding domain	0:24	The chitin-binding domain of a GH-18 chitinase from Vibrio harveyi	0:65	The chitin-binding domain of a GH-18 chitinase from Vibrio harveyi is crucial for chitin-chitinase interactions.
27667544	0	43	from	harveyi	59:65	arg1	crucial					70:76	crucial	70:76	crucial	70:76	The chitin-binding domain of a GH-18 chitinase from Vibrio harveyi is crucial for chitin-chitinase interactions.
27667544	0	43	from	harveyi	59:65	arg1	chitinase					37:45	a GH-18 chitinase	29:45	a GH-18 chitinase from Vibrio harveyi	29:65	The chitin-binding domain of a GH-18 chitinase from Vibrio harveyi is crucial for chitin-chitinase interactions.
27667544	3	44	theme	BL21	526:529	arg1	cells					559:563	E. coli BL21 strain Tuner(DE3)pLacI host cells	518:563	E. coli BL21 strain Tuner(DE3)pLacI host cells	518:563	The recombinant ChBDVhChiA was heterologously expressed in E. coli BL21 strain Tuner(DE3)pLacI host cells, and purified to homogeneity.
27667544	1	45	dep	domain	308:313	arg1	ii					272:273	ii	272:273	ii	272:273	Vibrio harveyi chitinase A (VhChiA) is a GH-18 glycosyl hydrolase with a structure containing three distinct domains: i) the N-terminal chitin-binding domain; ii) the (α/β)8 TIM barrel catalytic domain; and iii) the α+β insertion domain.
27667544	7	46	theme	isolated	1147:1154	arg1	domain					1156:1161	the isolated domain	1143:1161	the isolated domain	1143:1161	The binding affinity of the isolated domain was significantly lower than that of intact VhChiA, suggesting that the catalytic domain works synergistically with the chitin-binding domain to guide the polymeric substrate into the substrate binding cleft.
27667544	1	47	theme	α+β	329:331	arg1	domain					343:348	the α+β insertion domain	325:348	iii) the α+β insertion domain	320:348	Vibrio harveyi chitinase A (VhChiA) is a GH-18 glycosyl hydrolase with a structure containing three distinct domains: i) the N-terminal chitin-binding domain; ii) the (α/β)8 TIM barrel catalytic domain; and iii) the α+β insertion domain.
27667544	8	48	theme	bacterial	1457:1465	arg1	A					1483:1483	the marine bacterial GH-18 chitinase A	1446:1483	the marine bacterial GH-18 chitinase A	1446:1483	These data confirm the physiological role of the chitin-binding domain of the marine bacterial GH-18 chitinase A in chitin-chitinase interactions.
27667544	1	49	theme	Vibrio	113:118	arg1	VhChiA					141:146	VhChiA	141:146	VhChiA	141:146	Vibrio harveyi chitinase A (VhChiA) is a GH-18 glycosyl hydrolase with a structure containing three distinct domains: i) the N-terminal chitin-binding domain; ii) the (α/β)8 TIM barrel catalytic domain; and iii) the α+β insertion domain.
27667544	1	49	theme	Vibrio	113:118	arg1	A					138:138	Vibrio harveyi chitinase A	113:138	Vibrio harveyi chitinase A (VhChiA)	113:147	Vibrio harveyi chitinase A (VhChiA) is a GH-18 glycosyl hydrolase with a structure containing three distinct domains: i) the N-terminal chitin-binding domain; ii) the (α/β)8 TIM barrel catalytic domain; and iii) the α+β insertion domain.
27667544	1	49	theme	Vibrio	113:118	arg1	hydrolase					169:177	a GH-18 glycosyl hydrolase	152:177	a GH-18 glycosyl hydrolase with a structure containing three distinct domains: i) the N-terminal chitin-binding domain; ii) the (α/β)8 TIM barrel catalytic domain; and iii) the α+β insertion domain	152:348	Vibrio harveyi chitinase A (VhChiA) is a GH-18 glycosyl hydrolase with a structure containing three distinct domains: i) the N-terminal chitin-binding domain; ii) the (α/β)8 TIM barrel catalytic domain; and iii) the α+β insertion domain.
27667544	0	50	theme	chitinase	37:45	arg1	domain					19:24	The chitin-binding domain	0:24	The chitin-binding domain of a GH-18 chitinase from Vibrio harveyi	0:65	The chitin-binding domain of a GH-18 chitinase from Vibrio harveyi is crucial for chitin-chitinase interactions.
27667544	0	50	theme	chitinase	37:45	arg1	crucial					70:76	crucial	70:76	crucial	70:76	The chitin-binding domain of a GH-18 chitinase from Vibrio harveyi is crucial for chitin-chitinase interactions.
27667544	0	50	theme	chitinase	37:45	arg1	chitinase					37:45	a GH-18 chitinase	29:45	a GH-18 chitinase from Vibrio harveyi	29:65	The chitin-binding domain of a GH-18 chitinase from Vibrio harveyi is crucial for chitin-chitinase interactions.
27667544	1	51	dep	domain	264:269	arg1	i					231:231	i	231:231	i	231:231	Vibrio harveyi chitinase A (VhChiA) is a GH-18 glycosyl hydrolase with a structure containing three distinct domains: i) the N-terminal chitin-binding domain; ii) the (α/β)8 TIM barrel catalytic domain; and iii) the α+β insertion domain.
27667544	1	52	with	hydrolase	169:177	arg1	structure					186:194	a structure	184:194	a structure containing three distinct domains: i) the N-terminal chitin-binding domain; ii) the (α/β)8 TIM barrel catalytic domain; and iii) the α+β insertion domain	184:348	Vibrio harveyi chitinase A (VhChiA) is a GH-18 glycosyl hydrolase with a structure containing three distinct domains: i) the N-terminal chitin-binding domain; ii) the (α/β)8 TIM barrel catalytic domain; and iii) the α+β insertion domain.
27667544	1	53	theme	insertion	333:341	arg1	domain					343:348	the α+β insertion domain	325:348	iii) the α+β insertion domain	320:348	Vibrio harveyi chitinase A (VhChiA) is a GH-18 glycosyl hydrolase with a structure containing three distinct domains: i) the N-terminal chitin-binding domain; ii) the (α/β)8 TIM barrel catalytic domain; and iii) the α+β insertion domain.
27667544	3	54	theme	E.	518:519	arg1	cells					559:563	E. coli BL21 strain Tuner(DE3)pLacI host cells	518:563	E. coli BL21 strain Tuner(DE3)pLacI host cells	518:563	The recombinant ChBDVhChiA was heterologously expressed in E. coli BL21 strain Tuner(DE3)pLacI host cells, and purified to homogeneity.
27667544	1	55	theme	harveyi	120:126	arg1	VhChiA					141:146	VhChiA	141:146	VhChiA	141:146	Vibrio harveyi chitinase A (VhChiA) is a GH-18 glycosyl hydrolase with a structure containing three distinct domains: i) the N-terminal chitin-binding domain; ii) the (α/β)8 TIM barrel catalytic domain; and iii) the α+β insertion domain.
27667544	1	55	theme	harveyi	120:126	arg1	A					138:138	Vibrio harveyi chitinase A	113:138	Vibrio harveyi chitinase A (VhChiA)	113:147	Vibrio harveyi chitinase A (VhChiA) is a GH-18 glycosyl hydrolase with a structure containing three distinct domains: i) the N-terminal chitin-binding domain; ii) the (α/β)8 TIM barrel catalytic domain; and iii) the α+β insertion domain.
27667544	1	55	theme	harveyi	120:126	arg1	hydrolase					169:177	a GH-18 glycosyl hydrolase	152:177	a GH-18 glycosyl hydrolase with a structure containing three distinct domains: i) the N-terminal chitin-binding domain; ii) the (α/β)8 TIM barrel catalytic domain; and iii) the α+β insertion domain	152:348	Vibrio harveyi chitinase A (VhChiA) is a GH-18 glycosyl hydrolase with a structure containing three distinct domains: i) the N-terminal chitin-binding domain; ii) the (α/β)8 TIM barrel catalytic domain; and iii) the α+β insertion domain.
27667544	1	56	theme	α/β	281:283	arg1	domain					308:313	ii) the (α/β)8 TIM barrel catalytic domain	272:313	ii) the (α/β)8 TIM barrel catalytic domain	272:313	Vibrio harveyi chitinase A (VhChiA) is a GH-18 glycosyl hydrolase with a structure containing three distinct domains: i) the N-terminal chitin-binding domain; ii) the (α/β)8 TIM barrel catalytic domain; and iii) the α+β insertion domain.
27667544	8	57	theme	domain	1436:1441	arg1	role					1409:1412	the physiological role	1391:1412	the physiological role of the chitin-binding domain of the marine bacterial GH-18 chitinase A in chitin-chitinase interactions	1391:1516	These data confirm the physiological role of the chitin-binding domain of the marine bacterial GH-18 chitinase A in chitin-chitinase interactions.
27667544	7	58	theme	chitin-binding	1283:1296	arg1	domain					1298:1303	the chitin-binding domain	1279:1303	the chitin-binding domain	1279:1303	The binding affinity of the isolated domain was significantly lower than that of intact VhChiA, suggesting that the catalytic domain works synergistically with the chitin-binding domain to guide the polymeric substrate into the substrate binding cleft.
27667544	7	59	theme	substrate	1347:1355	arg1	cleft					1365:1369	the substrate binding cleft	1343:1369	the substrate binding cleft	1343:1369	The binding affinity of the isolated domain was significantly lower than that of intact VhChiA, suggesting that the catalytic domain works synergistically with the chitin-binding domain to guide the polymeric substrate into the substrate binding cleft.
27667544	1	60	theme	chitinase	128:136	arg1	VhChiA					141:146	VhChiA	141:146	VhChiA	141:146	Vibrio harveyi chitinase A (VhChiA) is a GH-18 glycosyl hydrolase with a structure containing three distinct domains: i) the N-terminal chitin-binding domain; ii) the (α/β)8 TIM barrel catalytic domain; and iii) the α+β insertion domain.
27667544	1	60	theme	chitinase	128:136	arg1	A					138:138	Vibrio harveyi chitinase A	113:138	Vibrio harveyi chitinase A (VhChiA)	113:147	Vibrio harveyi chitinase A (VhChiA) is a GH-18 glycosyl hydrolase with a structure containing three distinct domains: i) the N-terminal chitin-binding domain; ii) the (α/β)8 TIM barrel catalytic domain; and iii) the α+β insertion domain.
27667544	1	60	theme	chitinase	128:136	arg1	hydrolase					169:177	a GH-18 glycosyl hydrolase	152:177	a GH-18 glycosyl hydrolase with a structure containing three distinct domains: i) the N-terminal chitin-binding domain; ii) the (α/β)8 TIM barrel catalytic domain; and iii) the α+β insertion domain	152:348	Vibrio harveyi chitinase A (VhChiA) is a GH-18 glycosyl hydrolase with a structure containing three distinct domains: i) the N-terminal chitin-binding domain; ii) the (α/β)8 TIM barrel catalytic domain; and iii) the α+β insertion domain.
27667544	7	61	theme	binding	1357:1363	arg1	cleft					1365:1369	the substrate binding cleft	1343:1369	the substrate binding cleft	1343:1369	The binding affinity of the isolated domain was significantly lower than that of intact VhChiA, suggesting that the catalytic domain works synergistically with the chitin-binding domain to guide the polymeric substrate into the substrate binding cleft.
27667544	8	62	theme	marine	1450:1455	arg1	A					1483:1483	the marine bacterial GH-18 chitinase A	1446:1483	the marine bacterial GH-18 chitinase A	1446:1483	These data confirm the physiological role of the chitin-binding domain of the marine bacterial GH-18 chitinase A in chitin-chitinase interactions.
27667544	3	63	theme	DE3	544:546	arg1	cells					559:563	E. coli BL21 strain Tuner(DE3)pLacI host cells	518:563	E. coli BL21 strain Tuner(DE3)pLacI host cells	518:563	The recombinant ChBDVhChiA was heterologously expressed in E. coli BL21 strain Tuner(DE3)pLacI host cells, and purified to homogeneity.
27667544	6	64	from	functionality	1032:1044	arg1	chains					1056:1061	chitin chains	1049:1061	chitin chains	1049:1061	These results identified the tandem N-acetamido functionality on chitin chains as the specific sites of enzyme-substrate interactions.
27667544	2	65	theme	chitin-binding	407:420	arg1	VhChiA					432:437	VhChiA	432:437	VhChiA	432:437	In this study, we cloned the gene fragment encoding the chitin-binding domain of VhChiA, termed ChBDVhChiA.
27667544	2	65	theme	chitin-binding	407:420	arg1	domain					422:427	the chitin-binding domain	403:427	the chitin-binding domain	403:427	In this study, we cloned the gene fragment encoding the chitin-binding domain of VhChiA, termed ChBDVhChiA.
27667544	6	66	theme	specific	1070:1077	arg1	sites					1079:1083	the specific sites	1066:1083	the specific sites of enzyme-substrate interactions	1066:1116	These results identified the tandem N-acetamido functionality on chitin chains as the specific sites of enzyme-substrate interactions.
27667544	6	66	theme	specific	1070:1077	arg1	interactions					1105:1116	enzyme-substrate interactions	1088:1116	enzyme-substrate interactions	1088:1116	These results identified the tandem N-acetamido functionality on chitin chains as the specific sites of enzyme-substrate interactions.
27667544	6	66	theme	specific	1070:1077	arg1	functionality					1032:1044	the tandem N-acetamido functionality	1009:1044	the tandem N-acetamido functionality on chitin chains	1009:1061	These results identified the tandem N-acetamido functionality on chitin chains as the specific sites of enzyme-substrate interactions.
27667544	1	67	theme	distinct	213:220	arg1	domains					222:228	three distinct domains	207:228	three distinct domains	207:228	Vibrio harveyi chitinase A (VhChiA) is a GH-18 glycosyl hydrolase with a structure containing three distinct domains: i) the N-terminal chitin-binding domain; ii) the (α/β)8 TIM barrel catalytic domain; and iii) the α+β insertion domain.
27667544	8	68	theme	chitin-binding	1421:1434	arg1	A					1483:1483	the marine bacterial GH-18 chitinase A	1446:1483	the marine bacterial GH-18 chitinase A	1446:1483	These data confirm the physiological role of the chitin-binding domain of the marine bacterial GH-18 chitinase A in chitin-chitinase interactions.
27667544	8	68	theme	chitin-binding	1421:1434	arg1	domain					1436:1441	the chitin-binding domain	1417:1441	the chitin-binding domain of the marine bacterial GH-18 chitinase A	1417:1483	These data confirm the physiological role of the chitin-binding domain of the marine bacterial GH-18 chitinase A in chitin-chitinase interactions.
27667544	3	69	theme	strain	531:536	arg1	cells					559:563	E. coli BL21 strain Tuner(DE3)pLacI host cells	518:563	E. coli BL21 strain Tuner(DE3)pLacI host cells	518:563	The recombinant ChBDVhChiA was heterologously expressed in E. coli BL21 strain Tuner(DE3)pLacI host cells, and purified to homogeneity.
27667544	5	70	theme	binding	823:829	arg1	assays					831:836	Chitin binding assays	816:836	Chitin binding assays	816:836	Chitin binding assays showed that ChBDVhChiA bound to both α- and β-chitins, with the greatest affinity for β-colloidal chitin, but barely bound to polymeric chitosan.
27667544	3	71	dep	E.	518:519	arg1	coli					521:524	coli	521:524	coli	521:524	The recombinant ChBDVhChiA was heterologously expressed in E. coli BL21 strain Tuner(DE3)pLacI host cells, and purified to homogeneity.
27667544	0	72	theme	Vibrio	52:57	arg1	harveyi					59:65	Vibrio harveyi	52:65	Vibrio harveyi	52:65	The chitin-binding domain of a GH-18 chitinase from Vibrio harveyi is crucial for chitin-chitinase interactions.
27667544	8	73	theme	GH-18	1467:1471	arg1	A					1483:1483	the marine bacterial GH-18 chitinase A	1446:1483	the marine bacterial GH-18 chitinase A	1446:1483	These data confirm the physiological role of the chitin-binding domain of the marine bacterial GH-18 chitinase A in chitin-chitinase interactions.
27667544	3	74	theme	Tuner	538:542	arg1	cells					559:563	E. coli BL21 strain Tuner(DE3)pLacI host cells	518:563	E. coli BL21 strain Tuner(DE3)pLacI host cells	518:563	The recombinant ChBDVhChiA was heterologously expressed in E. coli BL21 strain Tuner(DE3)pLacI host cells, and purified to homogeneity.
27667544	7	75	theme	binding	1123:1129	arg1	lower					1181:1185	lower	1181:1185	lower	1181:1185	The binding affinity of the isolated domain was significantly lower than that of intact VhChiA, suggesting that the catalytic domain works synergistically with the chitin-binding domain to guide the polymeric substrate into the substrate binding cleft.
27667544	7	75	theme	binding	1123:1129	arg1	affinity					1131:1138	The binding affinity	1119:1138	The binding affinity of the isolated domain	1119:1161	The binding affinity of the isolated domain was significantly lower than that of intact VhChiA, suggesting that the catalytic domain works synergistically with the chitin-binding domain to guide the polymeric substrate into the substrate binding cleft.
28360940	14	0	theme	major	2591:2595	arg1	polysaccharides					2597:2611	The major polysaccharides	2587:2611	The major polysaccharides	2587:2611	The major polysaccharides include 4-O-methyl-d-glucuronic acid-substituted xylan and pectic-arabinogalactan, suggesting that enzymes with glucuronidase and arabinofuranosidase activities are required to maximize monomeric sugar yields.
28360940	10	1	theme	glycan-specific	1765:1779	arg1	mAbs					1804:1807	mAbs	1804:1807	mAbs	1804:1807	Cell wall glycan-specific monoclonal antibodies (mAbs) were used to characterize the type and abundance of non-cellulosic polysaccharides present in UHS over the course of enzymatic hydrolysis.
28360940	10	1	theme	glycan-specific	1765:1779	arg1	antibodies					1792:1801	Cell wall glycan-specific monoclonal antibodies	1755:1801	Cell wall glycan-specific monoclonal antibodies (mAbs)	1755:1808	Cell wall glycan-specific monoclonal antibodies (mAbs) were used to characterize the type and abundance of non-cellulosic polysaccharides present in UHS over the course of enzymatic hydrolysis.
28360940	5	2	from	present	975:981	arg1	pectins					1005:1011	pectins	1005:1011	pectins	1005:1011	Current commercial enzyme cocktails are not effective to digest specific cross-linkages in plant cell wall glycans, especially some of those present in hemicelluloses and pectins.
28360940	5	2	from	present	975:981	arg1	hemicelluloses					986:999	hemicelluloses	986:999	hemicelluloses	986:999	Current commercial enzyme cocktails are not effective to digest specific cross-linkages in plant cell wall glycans, especially some of those present in hemicelluloses and pectins.
28360940	12	3	theme	first	2300:2304	arg1	report					2306:2311	the first report	2296:2311	the first report using glycome profiling as a tool to dynamically monitor recalcitrant cell wall carbohydrates during the course of enzymatic hydrolysis	2296:2447	CONCLUSION To our knowledge, this is the first report using glycome profiling as a tool to dynamically monitor recalcitrant cell wall carbohydrates during the course of enzymatic hydrolysis.
28360940	12	3	theme	first	2300:2304	arg1	this					2288:2291	this	2288:2291	this	2288:2291	CONCLUSION To our knowledge, this is the first report using glycome profiling as a tool to dynamically monitor recalcitrant cell wall carbohydrates during the course of enzymatic hydrolysis.
28360940	15	4	theme	enzyme	2966:2971	arg1	activities					2973:2982	the required enzyme activities	2953:2982	the required enzyme activities for achieving complete deconstruction of pretreated biomass in the future	2953:3056	This methodology provides a rapid tool to assist in developing new enzyme cocktails, by supplementing the existing cocktails with the required enzyme activities for achieving complete deconstruction of pretreated biomass in the future.
28360940	1	5	theme	ionic	315:319	arg1	pretreatments					328:340	ionic liquid pretreatments	315:340	ionic liquid pretreatments	315:340	BACKGROUND Inefficient carbohydrate conversion has been an unsolved problem for various lignocellulosic biomass pretreatment technologies, including AFEX, dilute acid, and ionic liquid pretreatments.
28360940	5	6	theme	cell	931:934	arg1	those					969:973	those	969:973	those	969:973	Current commercial enzyme cocktails are not effective to digest specific cross-linkages in plant cell wall glycans, especially some of those present in hemicelluloses and pectins.
28360940	5	6	theme	cell	931:934	arg1	glycans					941:947	plant cell wall glycans	925:947	plant cell wall glycans	925:947	Current commercial enzyme cocktails are not effective to digest specific cross-linkages in plant cell wall glycans, especially some of those present in hemicelluloses and pectins.
28360940	5	6	theme	cell	931:934	arg1	some					961:964	some	961:964	some	961:964	Current commercial enzyme cocktails are not effective to digest specific cross-linkages in plant cell wall glycans, especially some of those present in hemicelluloses and pectins.
28360940	11	7	theme	polysaccharide	2234:2247	arg1	residues					2249:2256	those substituted polysaccharide residues	2216:2256	those substituted polysaccharide residues	2216:2256	4-O-methyl-d-glucuronic acid-substituted xylan and pectic-arabinogalactan were found to be the most abundant epitopes recognized by mAbs in UHS and liquid hydrolysate, suggesting that the commercial enzyme cocktails used in this work are unable to effectively target those substituted polysaccharide residues.
28360940	5	8	from	pectins	1005:1011	arg1	present					975:981	present	975:981	present	975:981	Current commercial enzyme cocktails are not effective to digest specific cross-linkages in plant cell wall glycans, especially some of those present in hemicelluloses and pectins.
28360940	9	9	theme	168 h	1727:1731	arg1	hydrolysis					1743:1752	168 h enzymatic hydrolysis	1727:1752	168 h enzymatic hydrolysis	1727:1752	The carbohydrates present in UHS and liquid hydrolysate were evaluated over a time period of 168 h enzymatic hydrolysis.
28360940	6	10	theme	required	1199:1206	arg1	activities					1215:1224	the required enzyme activities	1195:1224	the required enzyme activities for hydrolyzing those unconverted glycans	1195:1266	Thus, obtaining information about the most recalcitrant non-cellulosic glycan cross-linkages becomes a key study to rationally improve commercial enzyme cocktails, by supplementing the required enzyme activities for hydrolyzing those unconverted glycans.
28360940	1	11	theme	lignocellulosic	231:245	arg1	technologies					268:279	various lignocellulosic biomass pretreatment technologies	223:279	various lignocellulosic biomass pretreatment technologies	223:279	BACKGROUND Inefficient carbohydrate conversion has been an unsolved problem for various lignocellulosic biomass pretreatment technologies, including AFEX, dilute acid, and ionic liquid pretreatments.
28360940	1	11	theme	lignocellulosic	231:245	arg1	AFEX					292:295	AFEX	292:295	AFEX	292:295	BACKGROUND Inefficient carbohydrate conversion has been an unsolved problem for various lignocellulosic biomass pretreatment technologies, including AFEX, dilute acid, and ionic liquid pretreatments.
28360940	1	11	theme	lignocellulosic	231:245	arg1	acid					305:308	dilute acid	298:308	dilute acid	298:308	BACKGROUND Inefficient carbohydrate conversion has been an unsolved problem for various lignocellulosic biomass pretreatment technologies, including AFEX, dilute acid, and ionic liquid pretreatments.
28360940	1	11	theme	lignocellulosic	231:245	arg1	pretreatments					328:340	ionic liquid pretreatments	315:340	ionic liquid pretreatments	315:340	BACKGROUND Inefficient carbohydrate conversion has been an unsolved problem for various lignocellulosic biomass pretreatment technologies, including AFEX, dilute acid, and ionic liquid pretreatments.
28360940	5	12	attach	present	975:981	arg1	hemicelluloses					986:999	hemicelluloses	986:999	hemicelluloses	986:999	Current commercial enzyme cocktails are not effective to digest specific cross-linkages in plant cell wall glycans, especially some of those present in hemicelluloses and pectins.
28360940	5	12	attach	present	975:981	arg1	pectins					1005:1011	pectins	1005:1011	pectins	1005:1011	Current commercial enzyme cocktails are not effective to digest specific cross-linkages in plant cell wall glycans, especially some of those present in hemicelluloses and pectins.
28360940	5	12	attach	present	975:981	arg2	those					969:973	those	969:973	those	969:973	Current commercial enzyme cocktails are not effective to digest specific cross-linkages in plant cell wall glycans, especially some of those present in hemicelluloses and pectins.
28360940	9	13	theme	hydrolysis	1743:1752	arg1	period					1717:1722	a time period	1710:1722	a time period of 168 h enzymatic hydrolysis	1710:1752	The carbohydrates present in UHS and liquid hydrolysate were evaluated over a time period of 168 h enzymatic hydrolysis.
28360940	10	14	attach	present	1893:1899	arg2	polysaccharides					1877:1891	non-cellulosic polysaccharides	1862:1891	non-cellulosic polysaccharides present in UHS	1862:1906	Cell wall glycan-specific monoclonal antibodies (mAbs) were used to characterize the type and abundance of non-cellulosic polysaccharides present in UHS over the course of enzymatic hydrolysis.
28360940	10	14	attach	present	1893:1899	arg1	UHS					1904:1906	UHS	1904:1906	UHS	1904:1906	Cell wall glycan-specific monoclonal antibodies (mAbs) were used to characterize the type and abundance of non-cellulosic polysaccharides present in UHS over the course of enzymatic hydrolysis.
28360940	5	15	theme	enzyme	853:858	arg1	cocktails					860:868	Current commercial enzyme cocktails	834:868	Current commercial enzyme cocktails	834:868	Current commercial enzyme cocktails are not effective to digest specific cross-linkages in plant cell wall glycans, especially some of those present in hemicelluloses and pectins.
28360940	1	16	theme	Inefficient	154:164	arg1	problem					211:217	an unsolved problem	199:217	an unsolved problem for various lignocellulosic biomass pretreatment technologies, including AFEX, dilute acid, and ionic liquid pretreatments	199:340	BACKGROUND Inefficient carbohydrate conversion has been an unsolved problem for various lignocellulosic biomass pretreatment technologies, including AFEX, dilute acid, and ionic liquid pretreatments.
28360940	1	16	theme	Inefficient	154:164	arg1	conversion					179:188	BACKGROUND Inefficient carbohydrate conversion	143:188	BACKGROUND Inefficient carbohydrate conversion	143:188	BACKGROUND Inefficient carbohydrate conversion has been an unsolved problem for various lignocellulosic biomass pretreatment technologies, including AFEX, dilute acid, and ionic liquid pretreatments.
28360940	11	17	theme	commercial	2137:2146	arg1	cocktails					2155:2163	the commercial enzyme cocktails	2133:2163	the commercial enzyme cocktails used in this work	2133:2181	4-O-methyl-d-glucuronic acid-substituted xylan and pectic-arabinogalactan were found to be the most abundant epitopes recognized by mAbs in UHS and liquid hydrolysate, suggesting that the commercial enzyme cocktails used in this work are unable to effectively target those substituted polysaccharide residues.
28360940	11	17	theme	commercial	2137:2146	arg1	unable					2187:2192	unable	2187:2192	unable	2187:2192	4-O-methyl-d-glucuronic acid-substituted xylan and pectic-arabinogalactan were found to be the most abundant epitopes recognized by mAbs in UHS and liquid hydrolysate, suggesting that the commercial enzyme cocktails used in this work are unable to effectively target those substituted polysaccharide residues.
28360940	0	18	from	solids	102:107	arg1	characterization					14:29	Comprehensive characterization	0:29	Comprehensive characterization of non-cellulosic recalcitrant cell wall carbohydrates in unhydrolyzed solids from AFEX-pretreated corn stover.	0:141	Comprehensive characterization of non-cellulosic recalcitrant cell wall carbohydrates in unhydrolyzed solids from AFEX-pretreated corn stover.
28360940	15	19	theme	biomass	3036:3042	arg1	deconstruction					3007:3020	complete deconstruction	2998:3020	complete deconstruction of pretreated biomass in the future	2998:3056	This methodology provides a rapid tool to assist in developing new enzyme cocktails, by supplementing the existing cocktails with the required enzyme activities for achieving complete deconstruction of pretreated biomass in the future.
28360940	3	20	theme	unconverted	601:611	arg1	carbohydrates					613:625	these total unconverted carbohydrates	589:625	these total unconverted carbohydrates	589:625	Nearly one third (7 out of 22%) of these total unconverted carbohydrates are present in unhydrolyzed solid (UHS) residues.
28360940	10	21	used	used	1815:1818	arg2	mAbs					1804:1807	mAbs	1804:1807	mAbs	1804:1807	Cell wall glycan-specific monoclonal antibodies (mAbs) were used to characterize the type and abundance of non-cellulosic polysaccharides present in UHS over the course of enzymatic hydrolysis.
28360940	10	21	used	used	1815:1818	arg2	antibodies					1792:1801	Cell wall glycan-specific monoclonal antibodies	1755:1801	Cell wall glycan-specific monoclonal antibodies (mAbs)	1755:1808	Cell wall glycan-specific monoclonal antibodies (mAbs) were used to characterize the type and abundance of non-cellulosic polysaccharides present in UHS over the course of enzymatic hydrolysis.
28360940	8	22	theme	commercial	1525:1534	arg1	mixtures					1543:1550	commercial enzyme mixtures	1525:1550	commercial enzyme mixtures comprising cellulase and hemicellulase at 7% glucan loading (~20% solid loading)	1525:1631	The pretreated CS was hydrolyzed using commercial enzyme mixtures comprising cellulase and hemicellulase at 7% glucan loading (~20% solid loading).
28360940	5	23	theme	wall	936:939	arg1	those					969:973	those	969:973	those	969:973	Current commercial enzyme cocktails are not effective to digest specific cross-linkages in plant cell wall glycans, especially some of those present in hemicelluloses and pectins.
28360940	5	23	theme	wall	936:939	arg1	glycans					941:947	plant cell wall glycans	925:947	plant cell wall glycans	925:947	Current commercial enzyme cocktails are not effective to digest specific cross-linkages in plant cell wall glycans, especially some of those present in hemicelluloses and pectins.
28360940	5	23	theme	wall	936:939	arg1	some					961:964	some	961:964	some	961:964	Current commercial enzyme cocktails are not effective to digest specific cross-linkages in plant cell wall glycans, especially some of those present in hemicelluloses and pectins.
28360940	0	24	from	stover	135:140	arg1	solids					102:107	unhydrolyzed solids	89:107	unhydrolyzed solids from AFEX-pretreated corn stover	89:140	Comprehensive characterization of non-cellulosic recalcitrant cell wall carbohydrates in unhydrolyzed solids from AFEX-pretreated corn stover.
28360940	0	24	from	stover	135:140	arg1	carbohydrates					72:84	non-cellulosic recalcitrant cell wall carbohydrates	34:84	non-cellulosic recalcitrant cell wall carbohydrates in unhydrolyzed solids from AFEX-pretreated corn stover	34:140	Comprehensive characterization of non-cellulosic recalcitrant cell wall carbohydrates in unhydrolyzed solids from AFEX-pretreated corn stover.
28360940	10	25	dep	type	1840:1843	arg1	the					1836:1838	the	1836:1838	the	1836:1838	Cell wall glycan-specific monoclonal antibodies (mAbs) were used to characterize the type and abundance of non-cellulosic polysaccharides present in UHS over the course of enzymatic hydrolysis.
28360940	15	26	theme	complete	2998:3005	arg1	deconstruction					3007:3020	complete deconstruction	2998:3020	complete deconstruction of pretreated biomass in the future	2998:3056	This methodology provides a rapid tool to assist in developing new enzyme cocktails, by supplementing the existing cocktails with the required enzyme activities for achieving complete deconstruction of pretreated biomass in the future.
28360940	11	27	theme	liquid	2097:2102	arg1	hydrolysate					2104:2114	liquid hydrolysate	2097:2114	liquid hydrolysate	2097:2114	4-O-methyl-d-glucuronic acid-substituted xylan and pectic-arabinogalactan were found to be the most abundant epitopes recognized by mAbs in UHS and liquid hydrolysate, suggesting that the commercial enzyme cocktails used in this work are unable to effectively target those substituted polysaccharide residues.
28360940	6	28	theme	unconverted	1248:1258	arg1	glycans					1260:1266	those unconverted glycans	1242:1266	those unconverted glycans	1242:1266	Thus, obtaining information about the most recalcitrant non-cellulosic glycan cross-linkages becomes a key study to rationally improve commercial enzyme cocktails, by supplementing the required enzyme activities for hydrolyzing those unconverted glycans.
28360940	10	29	theme	polysaccharides	1877:1891	arg1	abundance					1849:1857	abundance	1849:1857	abundance	1849:1857	Cell wall glycan-specific monoclonal antibodies (mAbs) were used to characterize the type and abundance of non-cellulosic polysaccharides present in UHS over the course of enzymatic hydrolysis.
28360940	10	29	theme	polysaccharides	1877:1891	arg1	type					1840:1843	type	1840:1843	type	1840:1843	Cell wall glycan-specific monoclonal antibodies (mAbs) were used to characterize the type and abundance of non-cellulosic polysaccharides present in UHS over the course of enzymatic hydrolysis.
28360940	2	30	theme	20 mg	528:532	arg1	biomass					544:550	20 mg enzymes/g biomass	528:550	20 mg enzymes/g biomass	528:550	Previous work has shown 22% of total carbohydrates are typically unconverted, remaining as soluble or insoluble oligomers after hydrolysis (72 h) with excess commercial enzyme loading (20 mg enzymes/g biomass).
28360940	2	30	theme	20 mg	528:532	arg1	loading					519:525	excess commercial enzyme loading	494:525	excess commercial enzyme loading (20 mg enzymes/g biomass)	494:551	Previous work has shown 22% of total carbohydrates are typically unconverted, remaining as soluble or insoluble oligomers after hydrolysis (72 h) with excess commercial enzyme loading (20 mg enzymes/g biomass).
28360940	12	31	theme	recalcitrant	2370:2381	arg1	carbohydrates					2393:2405	recalcitrant cell wall carbohydrates	2370:2405	recalcitrant cell wall carbohydrates	2370:2405	CONCLUSION To our knowledge, this is the first report using glycome profiling as a tool to dynamically monitor recalcitrant cell wall carbohydrates during the course of enzymatic hydrolysis.
28360940	4	32	theme	unconverted	699:709	arg1	carbohydrates					711:723	these unconverted carbohydrates	693:723	these unconverted carbohydrates	693:723	The presence of these unconverted carbohydrates leads to a considerable sugar yield loss, which negatively impacts the overall economics of the biorefinery.
28360940	1	33	theme	unsolved	202:209	arg1	problem					211:217	an unsolved problem	199:217	an unsolved problem for various lignocellulosic biomass pretreatment technologies, including AFEX, dilute acid, and ionic liquid pretreatments	199:340	BACKGROUND Inefficient carbohydrate conversion has been an unsolved problem for various lignocellulosic biomass pretreatment technologies, including AFEX, dilute acid, and ionic liquid pretreatments.
28360940	1	33	theme	unsolved	202:209	arg1	conversion					179:188	BACKGROUND Inefficient carbohydrate conversion	143:188	BACKGROUND Inefficient carbohydrate conversion	143:188	BACKGROUND Inefficient carbohydrate conversion has been an unsolved problem for various lignocellulosic biomass pretreatment technologies, including AFEX, dilute acid, and ionic liquid pretreatments.
28360940	12	34	theme	wall	2388:2391	arg1	carbohydrates					2393:2405	recalcitrant cell wall carbohydrates	2370:2405	recalcitrant cell wall carbohydrates	2370:2405	CONCLUSION To our knowledge, this is the first report using glycome profiling as a tool to dynamically monitor recalcitrant cell wall carbohydrates during the course of enzymatic hydrolysis.
28360940	0	35	theme	AFEX-pretreated	114:128	arg1	stover					135:140	AFEX-pretreated corn stover	114:140	AFEX-pretreated corn stover	114:140	Comprehensive characterization of non-cellulosic recalcitrant cell wall carbohydrates in unhydrolyzed solids from AFEX-pretreated corn stover.
28360940	7	36	from	stover	1397:1402	arg1	sugars					1364:1369	monomeric sugars	1354:1369	monomeric sugars from AFEX-pretreated corn stover (CS)	1354:1407	RESULTS In this work, cell wall glycans that could not be enzymatically converted to monomeric sugars from AFEX-pretreated corn stover (CS) were characterized using compositional analysis and glycome profiling tools.
28360940	8	37	theme	7	1594:1594	arg1	%					1595:1595	%	1595:1595	%	1595:1595	The pretreated CS was hydrolyzed using commercial enzyme mixtures comprising cellulase and hemicellulase at 7% glucan loading (~20% solid loading).
28360940	7	38	theme	monomeric	1354:1362	arg1	sugars					1364:1369	monomeric sugars	1354:1369	monomeric sugars from AFEX-pretreated corn stover (CS)	1354:1407	RESULTS In this work, cell wall glycans that could not be enzymatically converted to monomeric sugars from AFEX-pretreated corn stover (CS) were characterized using compositional analysis and glycome profiling tools.
28360940	15	39	theme	enzyme	2890:2895	arg1	cocktails					2897:2905	new enzyme cocktails	2886:2905	new enzyme cocktails	2886:2905	This methodology provides a rapid tool to assist in developing new enzyme cocktails, by supplementing the existing cocktails with the required enzyme activities for achieving complete deconstruction of pretreated biomass in the future.
28360940	7	40	theme	corn	1392:1395	arg1	CS					1405:1406	CS	1405:1406	CS	1405:1406	RESULTS In this work, cell wall glycans that could not be enzymatically converted to monomeric sugars from AFEX-pretreated corn stover (CS) were characterized using compositional analysis and glycome profiling tools.
28360940	7	40	theme	corn	1392:1395	arg1	stover					1397:1402	AFEX-pretreated corn stover	1376:1402	AFEX-pretreated corn stover (CS)	1376:1407	RESULTS In this work, cell wall glycans that could not be enzymatically converted to monomeric sugars from AFEX-pretreated corn stover (CS) were characterized using compositional analysis and glycome profiling tools.
28360940	8	41	theme	glucan	1597:1602	arg1	loading					1624:1630	~20% solid loading	1613:1630	~20% solid loading	1613:1630	The pretreated CS was hydrolyzed using commercial enzyme mixtures comprising cellulase and hemicellulase at 7% glucan loading (~20% solid loading).
28360940	8	41	theme	glucan	1597:1602	arg1	loading					1604:1610	7% glucan loading	1594:1610	7% glucan loading (~20% solid loading)	1594:1631	The pretreated CS was hydrolyzed using commercial enzyme mixtures comprising cellulase and hemicellulase at 7% glucan loading (~20% solid loading).
28360940	4	42	theme	sugar	749:753	arg1	loss					761:764	a considerable sugar yield loss	734:764	a considerable sugar yield loss	734:764	The presence of these unconverted carbohydrates leads to a considerable sugar yield loss, which negatively impacts the overall economics of the biorefinery.
28360940	11	43	theme	acid-substituted	1973:1988	arg1	pectic-arabinogalactan					2000:2021	pectic-arabinogalactan	2000:2021	pectic-arabinogalactan	2000:2021	4-O-methyl-d-glucuronic acid-substituted xylan and pectic-arabinogalactan were found to be the most abundant epitopes recognized by mAbs in UHS and liquid hydrolysate, suggesting that the commercial enzyme cocktails used in this work are unable to effectively target those substituted polysaccharide residues.
28360940	11	43	theme	acid-substituted	1973:1988	arg1	epitopes					2058:2065	the most abundant epitopes	2040:2065	the most abundant epitopes recognized by mAbs in UHS and liquid hydrolysate	2040:2114	4-O-methyl-d-glucuronic acid-substituted xylan and pectic-arabinogalactan were found to be the most abundant epitopes recognized by mAbs in UHS and liquid hydrolysate, suggesting that the commercial enzyme cocktails used in this work are unable to effectively target those substituted polysaccharide residues.
28360940	11	43	theme	acid-substituted	1973:1988	arg1	xylan					1990:1994	4-O-methyl-d-glucuronic acid-substituted xylan	1949:1994	4-O-methyl-d-glucuronic acid-substituted xylan	1949:1994	4-O-methyl-d-glucuronic acid-substituted xylan and pectic-arabinogalactan were found to be the most abundant epitopes recognized by mAbs in UHS and liquid hydrolysate, suggesting that the commercial enzyme cocktails used in this work are unable to effectively target those substituted polysaccharide residues.
28360940	0	44	theme	Comprehensive	0:12	arg1	characterization					14:29	Comprehensive characterization	0:29	Comprehensive characterization of non-cellulosic recalcitrant cell wall carbohydrates in unhydrolyzed solids from AFEX-pretreated corn stover.	0:141	Comprehensive characterization of non-cellulosic recalcitrant cell wall carbohydrates in unhydrolyzed solids from AFEX-pretreated corn stover.
28360940	1	45	theme	liquid	321:326	arg1	pretreatments					328:340	ionic liquid pretreatments	315:340	ionic liquid pretreatments	315:340	BACKGROUND Inefficient carbohydrate conversion has been an unsolved problem for various lignocellulosic biomass pretreatment technologies, including AFEX, dilute acid, and ionic liquid pretreatments.
28360940	10	46	from	present	1893:1899	arg1	UHS					1904:1906	UHS	1904:1906	UHS	1904:1906	Cell wall glycan-specific monoclonal antibodies (mAbs) were used to characterize the type and abundance of non-cellulosic polysaccharides present in UHS over the course of enzymatic hydrolysis.
28360940	10	47	theme	present	1893:1899	arg1	polysaccharides					1877:1891	non-cellulosic polysaccharides	1862:1891	non-cellulosic polysaccharides present in UHS	1862:1906	Cell wall glycan-specific monoclonal antibodies (mAbs) were used to characterize the type and abundance of non-cellulosic polysaccharides present in UHS over the course of enzymatic hydrolysis.
28360940	7	48	dep	RESULTS	1269:1275	arg1	characterized					1414:1426	characterized	1414:1426	were characterized using compositional analysis and glycome profiling tools	1409:1483	RESULTS In this work, cell wall glycans that could not be enzymatically converted to monomeric sugars from AFEX-pretreated corn stover (CS) were characterized using compositional analysis and glycome profiling tools.
28360940	5	49	theme	Current	834:840	arg1	cocktails					860:868	Current commercial enzyme cocktails	834:868	Current commercial enzyme cocktails	834:868	Current commercial enzyme cocktails are not effective to digest specific cross-linkages in plant cell wall glycans, especially some of those present in hemicelluloses and pectins.
28360940	10	50	from	UHS	1904:1906	arg1	present					1893:1899	present	1893:1899	present	1893:1899	Cell wall glycan-specific monoclonal antibodies (mAbs) were used to characterize the type and abundance of non-cellulosic polysaccharides present in UHS over the course of enzymatic hydrolysis.
28360940	6	51	theme	non-cellulosic	1070:1083	arg1	cross-linkages					1092:1105	the most recalcitrant non-cellulosic glycan cross-linkages	1048:1105	the most recalcitrant non-cellulosic glycan cross-linkages	1048:1105	Thus, obtaining information about the most recalcitrant non-cellulosic glycan cross-linkages becomes a key study to rationally improve commercial enzyme cocktails, by supplementing the required enzyme activities for hydrolyzing those unconverted glycans.
28360940	8	52	theme	%	1616:1616	arg1	loading					1624:1630	~20% solid loading	1613:1630	~20% solid loading	1613:1630	The pretreated CS was hydrolyzed using commercial enzyme mixtures comprising cellulase and hemicellulase at 7% glucan loading (~20% solid loading).
28360940	8	52	theme	%	1616:1616	arg1	loading					1604:1610	7% glucan loading	1594:1610	7% glucan loading (~20% solid loading)	1594:1631	The pretreated CS was hydrolyzed using commercial enzyme mixtures comprising cellulase and hemicellulase at 7% glucan loading (~20% solid loading).
28360940	3	53	theme	UHS	662:664	arg1	residues					667:674	unhydrolyzed solid (UHS) residues	642:674	unhydrolyzed solid (UHS) residues	642:674	Nearly one third (7 out of 22%) of these total unconverted carbohydrates are present in unhydrolyzed solid (UHS) residues.
28360940	4	54	theme	biorefinery	821:831	arg1	economics					804:812	the overall economics	792:812	the overall economics of the biorefinery	792:831	The presence of these unconverted carbohydrates leads to a considerable sugar yield loss, which negatively impacts the overall economics of the biorefinery.
28360940	0	55	theme	recalcitrant	49:60	arg1	carbohydrates					72:84	non-cellulosic recalcitrant cell wall carbohydrates	34:84	non-cellulosic recalcitrant cell wall carbohydrates in unhydrolyzed solids from AFEX-pretreated corn stover	34:140	Comprehensive characterization of non-cellulosic recalcitrant cell wall carbohydrates in unhydrolyzed solids from AFEX-pretreated corn stover.
28360940	2	56	theme	enzyme	512:517	arg1	biomass					544:550	20 mg enzymes/g biomass	528:550	20 mg enzymes/g biomass	528:550	Previous work has shown 22% of total carbohydrates are typically unconverted, remaining as soluble or insoluble oligomers after hydrolysis (72 h) with excess commercial enzyme loading (20 mg enzymes/g biomass).
28360940	2	56	theme	enzyme	512:517	arg1	loading					519:525	excess commercial enzyme loading	494:525	excess commercial enzyme loading (20 mg enzymes/g biomass)	494:551	Previous work has shown 22% of total carbohydrates are typically unconverted, remaining as soluble or insoluble oligomers after hydrolysis (72 h) with excess commercial enzyme loading (20 mg enzymes/g biomass).
28360940	9	57	theme	present	1652:1658	arg1	carbohydrates					1638:1650	The carbohydrates	1634:1650	The carbohydrates present in UHS and liquid hydrolysate	1634:1688	The carbohydrates present in UHS and liquid hydrolysate were evaluated over a time period of 168 h enzymatic hydrolysis.
28360940	2	58	theme	commercial	501:510	arg1	biomass					544:550	20 mg enzymes/g biomass	528:550	20 mg enzymes/g biomass	528:550	Previous work has shown 22% of total carbohydrates are typically unconverted, remaining as soluble or insoluble oligomers after hydrolysis (72 h) with excess commercial enzyme loading (20 mg enzymes/g biomass).
28360940	2	58	theme	commercial	501:510	arg1	loading					519:525	excess commercial enzyme loading	494:525	excess commercial enzyme loading (20 mg enzymes/g biomass)	494:551	Previous work has shown 22% of total carbohydrates are typically unconverted, remaining as soluble or insoluble oligomers after hydrolysis (72 h) with excess commercial enzyme loading (20 mg enzymes/g biomass).
28360940	3	59	theme	solid	655:659	arg1	residues					667:674	unhydrolyzed solid (UHS) residues	642:674	unhydrolyzed solid (UHS) residues	642:674	Nearly one third (7 out of 22%) of these total unconverted carbohydrates are present in unhydrolyzed solid (UHS) residues.
28360940	11	60	theme	substituted	2222:2232	arg1	residues					2249:2256	those substituted polysaccharide residues	2216:2256	those substituted polysaccharide residues	2216:2256	4-O-methyl-d-glucuronic acid-substituted xylan and pectic-arabinogalactan were found to be the most abundant epitopes recognized by mAbs in UHS and liquid hydrolysate, suggesting that the commercial enzyme cocktails used in this work are unable to effectively target those substituted polysaccharide residues.
28360940	2	61	theme	carbohydrates	380:392	arg1	%					369:369	22%	367:369	22% of total carbohydrates	367:392	Previous work has shown 22% of total carbohydrates are typically unconverted, remaining as soluble or insoluble oligomers after hydrolysis (72 h) with excess commercial enzyme loading (20 mg enzymes/g biomass).
28360940	2	61	theme	carbohydrates	380:392	arg1	carbohydrates					380:392	total carbohydrates	374:392	total carbohydrates	374:392	Previous work has shown 22% of total carbohydrates are typically unconverted, remaining as soluble or insoluble oligomers after hydrolysis (72 h) with excess commercial enzyme loading (20 mg enzymes/g biomass).
28360940	0	62	theme	wall	67:70	arg1	carbohydrates					72:84	non-cellulosic recalcitrant cell wall carbohydrates	34:84	non-cellulosic recalcitrant cell wall carbohydrates in unhydrolyzed solids from AFEX-pretreated corn stover	34:140	Comprehensive characterization of non-cellulosic recalcitrant cell wall carbohydrates in unhydrolyzed solids from AFEX-pretreated corn stover.
28360940	4	63	theme	overall	796:802	arg1	economics					804:812	the overall economics	792:812	the overall economics of the biorefinery	792:831	The presence of these unconverted carbohydrates leads to a considerable sugar yield loss, which negatively impacts the overall economics of the biorefinery.
28360940	13	64	theme	hydrolysates	2486:2497	arg1	profiling					2458:2466	Glycome profiling	2450:2466	Glycome profiling of UHS and liquid hydrolysates	2450:2497	Glycome profiling of UHS and liquid hydrolysates unveiled some of the glycans that are not cleaved and enriched after enzyme hydrolysis.
28360940	3	65	located	present	631:637	arg1	residues					667:674	unhydrolyzed solid (UHS) residues	642:674	unhydrolyzed solid (UHS) residues	642:674	Nearly one third (7 out of 22%) of these total unconverted carbohydrates are present in unhydrolyzed solid (UHS) residues.
28360940	3	65	located	present	631:637	arg2	%					583:583	7 out of 22%	572:583	7 out of 22%	572:583	Nearly one third (7 out of 22%) of these total unconverted carbohydrates are present in unhydrolyzed solid (UHS) residues.
28360940	3	65	located	present	631:637	arg2	third					565:569	third	565:569	third	565:569	Nearly one third (7 out of 22%) of these total unconverted carbohydrates are present in unhydrolyzed solid (UHS) residues.
28360940	11	66	theme	abundant	2049:2056	arg1	pectic-arabinogalactan					2000:2021	pectic-arabinogalactan	2000:2021	pectic-arabinogalactan	2000:2021	4-O-methyl-d-glucuronic acid-substituted xylan and pectic-arabinogalactan were found to be the most abundant epitopes recognized by mAbs in UHS and liquid hydrolysate, suggesting that the commercial enzyme cocktails used in this work are unable to effectively target those substituted polysaccharide residues.
28360940	11	66	theme	abundant	2049:2056	arg1	epitopes					2058:2065	the most abundant epitopes	2040:2065	the most abundant epitopes recognized by mAbs in UHS and liquid hydrolysate	2040:2114	4-O-methyl-d-glucuronic acid-substituted xylan and pectic-arabinogalactan were found to be the most abundant epitopes recognized by mAbs in UHS and liquid hydrolysate, suggesting that the commercial enzyme cocktails used in this work are unable to effectively target those substituted polysaccharide residues.
28360940	11	66	theme	abundant	2049:2056	arg1	xylan					1990:1994	4-O-methyl-d-glucuronic acid-substituted xylan	1949:1994	4-O-methyl-d-glucuronic acid-substituted xylan	1949:1994	4-O-methyl-d-glucuronic acid-substituted xylan and pectic-arabinogalactan were found to be the most abundant epitopes recognized by mAbs in UHS and liquid hydrolysate, suggesting that the commercial enzyme cocktails used in this work are unable to effectively target those substituted polysaccharide residues.
28360940	9	67	theme	liquid	1671:1676	arg1	hydrolysate					1678:1688	liquid hydrolysate	1671:1688	liquid hydrolysate	1671:1688	The carbohydrates present in UHS and liquid hydrolysate were evaluated over a time period of 168 h enzymatic hydrolysis.
28360940	6	68	theme	commercial	1149:1158	arg1	cocktails					1167:1175	commercial enzyme cocktails	1149:1175	commercial enzyme cocktails	1149:1175	Thus, obtaining information about the most recalcitrant non-cellulosic glycan cross-linkages becomes a key study to rationally improve commercial enzyme cocktails, by supplementing the required enzyme activities for hydrolyzing those unconverted glycans.
28360940	10	69	theme	enzymatic	1927:1935	arg1	hydrolysis					1937:1946	enzymatic hydrolysis	1927:1946	enzymatic hydrolysis	1927:1946	Cell wall glycan-specific monoclonal antibodies (mAbs) were used to characterize the type and abundance of non-cellulosic polysaccharides present in UHS over the course of enzymatic hydrolysis.
28360940	8	70	theme	pretreated	1490:1499	arg1	CS					1501:1502	The pretreated CS	1486:1502	The pretreated CS	1486:1502	The pretreated CS was hydrolyzed using commercial enzyme mixtures comprising cellulase and hemicellulase at 7% glucan loading (~20% solid loading).
28360940	10	71	theme	monoclonal	1781:1790	arg1	mAbs					1804:1807	mAbs	1804:1807	mAbs	1804:1807	Cell wall glycan-specific monoclonal antibodies (mAbs) were used to characterize the type and abundance of non-cellulosic polysaccharides present in UHS over the course of enzymatic hydrolysis.
28360940	10	71	theme	monoclonal	1781:1790	arg1	antibodies					1792:1801	Cell wall glycan-specific monoclonal antibodies	1755:1801	Cell wall glycan-specific monoclonal antibodies (mAbs)	1755:1808	Cell wall glycan-specific monoclonal antibodies (mAbs) were used to characterize the type and abundance of non-cellulosic polysaccharides present in UHS over the course of enzymatic hydrolysis.
28360940	7	72	theme	cell	1291:1294	arg1	glycans					1301:1307	cell wall glycans	1291:1307	cell wall glycans that could not be enzymatically converted to monomeric sugars from AFEX-pretreated corn stover (CS)	1291:1407	RESULTS In this work, cell wall glycans that could not be enzymatically converted to monomeric sugars from AFEX-pretreated corn stover (CS) were characterized using compositional analysis and glycome profiling tools.
28360940	1	73	theme	pretreatment	255:266	arg1	technologies					268:279	various lignocellulosic biomass pretreatment technologies	223:279	various lignocellulosic biomass pretreatment technologies	223:279	BACKGROUND Inefficient carbohydrate conversion has been an unsolved problem for various lignocellulosic biomass pretreatment technologies, including AFEX, dilute acid, and ionic liquid pretreatments.
28360940	1	73	theme	pretreatment	255:266	arg1	AFEX					292:295	AFEX	292:295	AFEX	292:295	BACKGROUND Inefficient carbohydrate conversion has been an unsolved problem for various lignocellulosic biomass pretreatment technologies, including AFEX, dilute acid, and ionic liquid pretreatments.
28360940	1	73	theme	pretreatment	255:266	arg1	acid					305:308	dilute acid	298:308	dilute acid	298:308	BACKGROUND Inefficient carbohydrate conversion has been an unsolved problem for various lignocellulosic biomass pretreatment technologies, including AFEX, dilute acid, and ionic liquid pretreatments.
28360940	1	73	theme	pretreatment	255:266	arg1	pretreatments					328:340	ionic liquid pretreatments	315:340	ionic liquid pretreatments	315:340	BACKGROUND Inefficient carbohydrate conversion has been an unsolved problem for various lignocellulosic biomass pretreatment technologies, including AFEX, dilute acid, and ionic liquid pretreatments.
28360940	15	74	theme	required	2957:2964	arg1	activities					2973:2982	the required enzyme activities	2953:2982	the required enzyme activities for achieving complete deconstruction of pretreated biomass in the future	2953:3056	This methodology provides a rapid tool to assist in developing new enzyme cocktails, by supplementing the existing cocktails with the required enzyme activities for achieving complete deconstruction of pretreated biomass in the future.
28360940	2	75	theme	Previous	343:350	arg1	work					352:355	Previous work	343:355	Previous work	343:355	Previous work has shown 22% of total carbohydrates are typically unconverted, remaining as soluble or insoluble oligomers after hydrolysis (72 h) with excess commercial enzyme loading (20 mg enzymes/g biomass).
28360940	7	76	theme	glycome	1461:1467	arg1	tools					1479:1483	glycome profiling tools	1461:1483	glycome profiling tools	1461:1483	RESULTS In this work, cell wall glycans that could not be enzymatically converted to monomeric sugars from AFEX-pretreated corn stover (CS) were characterized using compositional analysis and glycome profiling tools.
28360940	9	77	theme	time	1712:1715	arg1	period					1717:1722	a time period	1710:1722	a time period of 168 h enzymatic hydrolysis	1710:1752	The carbohydrates present in UHS and liquid hydrolysate were evaluated over a time period of 168 h enzymatic hydrolysis.
28360940	10	78	theme	wall	1760:1763	arg1	mAbs					1804:1807	mAbs	1804:1807	mAbs	1804:1807	Cell wall glycan-specific monoclonal antibodies (mAbs) were used to characterize the type and abundance of non-cellulosic polysaccharides present in UHS over the course of enzymatic hydrolysis.
28360940	10	78	theme	wall	1760:1763	arg1	antibodies					1792:1801	Cell wall glycan-specific monoclonal antibodies	1755:1801	Cell wall glycan-specific monoclonal antibodies (mAbs)	1755:1808	Cell wall glycan-specific monoclonal antibodies (mAbs) were used to characterize the type and abundance of non-cellulosic polysaccharides present in UHS over the course of enzymatic hydrolysis.
28360940	12	79	dep	CONCLUSION	2259:2268	arg1	knowledge					2277:2285	our knowledge	2273:2285	our knowledge	2273:2285	CONCLUSION To our knowledge, this is the first report using glycome profiling as a tool to dynamically monitor recalcitrant cell wall carbohydrates during the course of enzymatic hydrolysis.
28360940	15	80	theme	new	2886:2888	arg1	cocktails					2897:2905	new enzyme cocktails	2886:2905	new enzyme cocktails	2886:2905	This methodology provides a rapid tool to assist in developing new enzyme cocktails, by supplementing the existing cocktails with the required enzyme activities for achieving complete deconstruction of pretreated biomass in the future.
28360940	2	81	with	hydrolysis	471:480	arg1	biomass					544:550	20 mg enzymes/g biomass	528:550	20 mg enzymes/g biomass	528:550	Previous work has shown 22% of total carbohydrates are typically unconverted, remaining as soluble or insoluble oligomers after hydrolysis (72 h) with excess commercial enzyme loading (20 mg enzymes/g biomass).
28360940	2	81	with	hydrolysis	471:480	arg1	loading					519:525	excess commercial enzyme loading	494:525	excess commercial enzyme loading (20 mg enzymes/g biomass)	494:551	Previous work has shown 22% of total carbohydrates are typically unconverted, remaining as soluble or insoluble oligomers after hydrolysis (72 h) with excess commercial enzyme loading (20 mg enzymes/g biomass).
28360940	6	82	theme	enzyme	1208:1213	arg1	activities					1215:1224	the required enzyme activities	1195:1224	the required enzyme activities for hydrolyzing those unconverted glycans	1195:1266	Thus, obtaining information about the most recalcitrant non-cellulosic glycan cross-linkages becomes a key study to rationally improve commercial enzyme cocktails, by supplementing the required enzyme activities for hydrolyzing those unconverted glycans.
28360940	11	83	from	mAbs	2081:2084	arg1	UHS					2089:2091	UHS	2089:2091	UHS	2089:2091	4-O-methyl-d-glucuronic acid-substituted xylan and pectic-arabinogalactan were found to be the most abundant epitopes recognized by mAbs in UHS and liquid hydrolysate, suggesting that the commercial enzyme cocktails used in this work are unable to effectively target those substituted polysaccharide residues.
28360940	11	83	from	mAbs	2081:2084	arg1	hydrolysate					2104:2114	liquid hydrolysate	2097:2114	liquid hydrolysate	2097:2114	4-O-methyl-d-glucuronic acid-substituted xylan and pectic-arabinogalactan were found to be the most abundant epitopes recognized by mAbs in UHS and liquid hydrolysate, suggesting that the commercial enzyme cocktails used in this work are unable to effectively target those substituted polysaccharide residues.
28360940	9	84	attach	present	1652:1658	arg2	carbohydrates					1638:1650	The carbohydrates	1634:1650	The carbohydrates present in UHS and liquid hydrolysate	1634:1688	The carbohydrates present in UHS and liquid hydrolysate were evaluated over a time period of 168 h enzymatic hydrolysis.
28360940	9	84	attach	present	1652:1658	arg1	UHS					1663:1665	UHS	1663:1665	UHS	1663:1665	The carbohydrates present in UHS and liquid hydrolysate were evaluated over a time period of 168 h enzymatic hydrolysis.
28360940	9	84	attach	present	1652:1658	arg1	hydrolysate					1678:1688	liquid hydrolysate	1671:1688	liquid hydrolysate	1671:1688	The carbohydrates present in UHS and liquid hydrolysate were evaluated over a time period of 168 h enzymatic hydrolysis.
28360940	15	85	theme	existing	2929:2936	arg1	cocktails					2938:2946	the existing cocktails	2925:2946	the existing cocktails	2925:2946	This methodology provides a rapid tool to assist in developing new enzyme cocktails, by supplementing the existing cocktails with the required enzyme activities for achieving complete deconstruction of pretreated biomass in the future.
28360940	1	86	theme	various	223:229	arg1	technologies					268:279	various lignocellulosic biomass pretreatment technologies	223:279	various lignocellulosic biomass pretreatment technologies	223:279	BACKGROUND Inefficient carbohydrate conversion has been an unsolved problem for various lignocellulosic biomass pretreatment technologies, including AFEX, dilute acid, and ionic liquid pretreatments.
28360940	1	86	theme	various	223:229	arg1	AFEX					292:295	AFEX	292:295	AFEX	292:295	BACKGROUND Inefficient carbohydrate conversion has been an unsolved problem for various lignocellulosic biomass pretreatment technologies, including AFEX, dilute acid, and ionic liquid pretreatments.
28360940	1	86	theme	various	223:229	arg1	acid					305:308	dilute acid	298:308	dilute acid	298:308	BACKGROUND Inefficient carbohydrate conversion has been an unsolved problem for various lignocellulosic biomass pretreatment technologies, including AFEX, dilute acid, and ionic liquid pretreatments.
28360940	1	86	theme	various	223:229	arg1	pretreatments					328:340	ionic liquid pretreatments	315:340	ionic liquid pretreatments	315:340	BACKGROUND Inefficient carbohydrate conversion has been an unsolved problem for various lignocellulosic biomass pretreatment technologies, including AFEX, dilute acid, and ionic liquid pretreatments.
28360940	9	87	theme	enzymatic	1733:1741	arg1	hydrolysis					1743:1752	168 h enzymatic hydrolysis	1727:1752	168 h enzymatic hydrolysis	1727:1752	The carbohydrates present in UHS and liquid hydrolysate were evaluated over a time period of 168 h enzymatic hydrolysis.
28360940	7	88	theme	profiling	1469:1477	arg1	tools					1479:1483	glycome profiling tools	1461:1483	glycome profiling tools	1461:1483	RESULTS In this work, cell wall glycans that could not be enzymatically converted to monomeric sugars from AFEX-pretreated corn stover (CS) were characterized using compositional analysis and glycome profiling tools.
28360940	1	89	theme	biomass	247:253	arg1	technologies					268:279	various lignocellulosic biomass pretreatment technologies	223:279	various lignocellulosic biomass pretreatment technologies	223:279	BACKGROUND Inefficient carbohydrate conversion has been an unsolved problem for various lignocellulosic biomass pretreatment technologies, including AFEX, dilute acid, and ionic liquid pretreatments.
28360940	1	89	theme	biomass	247:253	arg1	AFEX					292:295	AFEX	292:295	AFEX	292:295	BACKGROUND Inefficient carbohydrate conversion has been an unsolved problem for various lignocellulosic biomass pretreatment technologies, including AFEX, dilute acid, and ionic liquid pretreatments.
28360940	1	89	theme	biomass	247:253	arg1	acid					305:308	dilute acid	298:308	dilute acid	298:308	BACKGROUND Inefficient carbohydrate conversion has been an unsolved problem for various lignocellulosic biomass pretreatment technologies, including AFEX, dilute acid, and ionic liquid pretreatments.
28360940	1	89	theme	biomass	247:253	arg1	pretreatments					328:340	ionic liquid pretreatments	315:340	ionic liquid pretreatments	315:340	BACKGROUND Inefficient carbohydrate conversion has been an unsolved problem for various lignocellulosic biomass pretreatment technologies, including AFEX, dilute acid, and ionic liquid pretreatments.
28360940	12	90	theme	hydrolysis	2438:2447	arg1	course					2418:2423	the course	2414:2423	the course of enzymatic hydrolysis	2414:2447	CONCLUSION To our knowledge, this is the first report using glycome profiling as a tool to dynamically monitor recalcitrant cell wall carbohydrates during the course of enzymatic hydrolysis.
28360940	5	91	theme	plant	925:929	arg1	those					969:973	those	969:973	those	969:973	Current commercial enzyme cocktails are not effective to digest specific cross-linkages in plant cell wall glycans, especially some of those present in hemicelluloses and pectins.
28360940	5	91	theme	plant	925:929	arg1	glycans					941:947	plant cell wall glycans	925:947	plant cell wall glycans	925:947	Current commercial enzyme cocktails are not effective to digest specific cross-linkages in plant cell wall glycans, especially some of those present in hemicelluloses and pectins.
28360940	5	91	theme	plant	925:929	arg1	some					961:964	some	961:964	some	961:964	Current commercial enzyme cocktails are not effective to digest specific cross-linkages in plant cell wall glycans, especially some of those present in hemicelluloses and pectins.
28360940	10	92	theme	Cell	1755:1758	arg1	mAbs					1804:1807	mAbs	1804:1807	mAbs	1804:1807	Cell wall glycan-specific monoclonal antibodies (mAbs) were used to characterize the type and abundance of non-cellulosic polysaccharides present in UHS over the course of enzymatic hydrolysis.
28360940	10	92	theme	Cell	1755:1758	arg1	antibodies					1792:1801	Cell wall glycan-specific monoclonal antibodies	1755:1801	Cell wall glycan-specific monoclonal antibodies (mAbs)	1755:1808	Cell wall glycan-specific monoclonal antibodies (mAbs) were used to characterize the type and abundance of non-cellulosic polysaccharides present in UHS over the course of enzymatic hydrolysis.
28360940	15	93	theme	pretreated	3025:3034	arg1	biomass					3036:3042	pretreated biomass	3025:3042	pretreated biomass	3025:3042	This methodology provides a rapid tool to assist in developing new enzyme cocktails, by supplementing the existing cocktails with the required enzyme activities for achieving complete deconstruction of pretreated biomass in the future.
28360940	3	94	theme	total	595:599	arg1	carbohydrates					613:625	these total unconverted carbohydrates	589:625	these total unconverted carbohydrates	589:625	Nearly one third (7 out of 22%) of these total unconverted carbohydrates are present in unhydrolyzed solid (UHS) residues.
28360940	13	95	theme	Glycome	2450:2456	arg1	profiling					2458:2466	Glycome profiling	2450:2466	Glycome profiling of UHS and liquid hydrolysates	2450:2497	Glycome profiling of UHS and liquid hydrolysates unveiled some of the glycans that are not cleaved and enriched after enzyme hydrolysis.
28360940	14	96	theme	monomeric	2799:2807	arg1	yields					2815:2820	monomeric sugar yields	2799:2820	monomeric sugar yields	2799:2820	The major polysaccharides include 4-O-methyl-d-glucuronic acid-substituted xylan and pectic-arabinogalactan, suggesting that enzymes with glucuronidase and arabinofuranosidase activities are required to maximize monomeric sugar yields.
28360940	11	97	theme	enzyme	2148:2153	arg1	cocktails					2155:2163	the commercial enzyme cocktails	2133:2163	the commercial enzyme cocktails used in this work	2133:2181	4-O-methyl-d-glucuronic acid-substituted xylan and pectic-arabinogalactan were found to be the most abundant epitopes recognized by mAbs in UHS and liquid hydrolysate, suggesting that the commercial enzyme cocktails used in this work are unable to effectively target those substituted polysaccharide residues.
28360940	11	97	theme	enzyme	2148:2153	arg1	unable					2187:2192	unable	2187:2192	unable	2187:2192	4-O-methyl-d-glucuronic acid-substituted xylan and pectic-arabinogalactan were found to be the most abundant epitopes recognized by mAbs in UHS and liquid hydrolysate, suggesting that the commercial enzyme cocktails used in this work are unable to effectively target those substituted polysaccharide residues.
28360940	7	98	theme	compositional	1434:1446	arg1	analysis					1448:1455	compositional analysis	1434:1455	compositional analysis	1434:1455	RESULTS In this work, cell wall glycans that could not be enzymatically converted to monomeric sugars from AFEX-pretreated corn stover (CS) were characterized using compositional analysis and glycome profiling tools.
28360940	1	99	theme	BACKGROUND	143:152	arg1	problem					211:217	an unsolved problem	199:217	an unsolved problem for various lignocellulosic biomass pretreatment technologies, including AFEX, dilute acid, and ionic liquid pretreatments	199:340	BACKGROUND Inefficient carbohydrate conversion has been an unsolved problem for various lignocellulosic biomass pretreatment technologies, including AFEX, dilute acid, and ionic liquid pretreatments.
28360940	1	99	theme	BACKGROUND	143:152	arg1	conversion					179:188	BACKGROUND Inefficient carbohydrate conversion	143:188	BACKGROUND Inefficient carbohydrate conversion	143:188	BACKGROUND Inefficient carbohydrate conversion has been an unsolved problem for various lignocellulosic biomass pretreatment technologies, including AFEX, dilute acid, and ionic liquid pretreatments.
28360940	8	100	theme	enzyme	1536:1541	arg1	mixtures					1543:1550	commercial enzyme mixtures	1525:1550	commercial enzyme mixtures comprising cellulase and hemicellulase at 7% glucan loading (~20% solid loading)	1525:1631	The pretreated CS was hydrolyzed using commercial enzyme mixtures comprising cellulase and hemicellulase at 7% glucan loading (~20% solid loading).
28360940	4	101	theme	carbohydrates	711:723	arg1	presence					681:688	The presence	677:688	The presence of these unconverted carbohydrates	677:723	The presence of these unconverted carbohydrates leads to a considerable sugar yield loss, which negatively impacts the overall economics of the biorefinery.
28360940	13	102	theme	enzyme	2568:2573	arg1	hydrolysis					2575:2584	enzyme hydrolysis	2568:2584	enzyme hydrolysis	2568:2584	Glycome profiling of UHS and liquid hydrolysates unveiled some of the glycans that are not cleaved and enriched after enzyme hydrolysis.
28360940	1	103	theme	carbohydrate	166:177	arg1	problem					211:217	an unsolved problem	199:217	an unsolved problem for various lignocellulosic biomass pretreatment technologies, including AFEX, dilute acid, and ionic liquid pretreatments	199:340	BACKGROUND Inefficient carbohydrate conversion has been an unsolved problem for various lignocellulosic biomass pretreatment technologies, including AFEX, dilute acid, and ionic liquid pretreatments.
28360940	1	103	theme	carbohydrate	166:177	arg1	conversion					179:188	BACKGROUND Inefficient carbohydrate conversion	143:188	BACKGROUND Inefficient carbohydrate conversion	143:188	BACKGROUND Inefficient carbohydrate conversion has been an unsolved problem for various lignocellulosic biomass pretreatment technologies, including AFEX, dilute acid, and ionic liquid pretreatments.
28360940	3	104	attach	present	631:637	arg2	third					565:569	third	565:569	third	565:569	Nearly one third (7 out of 22%) of these total unconverted carbohydrates are present in unhydrolyzed solid (UHS) residues.
28360940	3	104	attach	present	631:637	arg2	%					583:583	7 out of 22%	572:583	7 out of 22%	572:583	Nearly one third (7 out of 22%) of these total unconverted carbohydrates are present in unhydrolyzed solid (UHS) residues.
28360940	3	104	attach	present	631:637	arg1	residues					667:674	unhydrolyzed solid (UHS) residues	642:674	unhydrolyzed solid (UHS) residues	642:674	Nearly one third (7 out of 22%) of these total unconverted carbohydrates are present in unhydrolyzed solid (UHS) residues.
28360940	0	105	theme	unhydrolyzed	89:100	arg1	solids					102:107	unhydrolyzed solids	89:107	unhydrolyzed solids from AFEX-pretreated corn stover	89:140	Comprehensive characterization of non-cellulosic recalcitrant cell wall carbohydrates in unhydrolyzed solids from AFEX-pretreated corn stover.
28360940	3	106	theme	carbohydrates	613:625	arg1	third					565:569	third	565:569	third	565:569	Nearly one third (7 out of 22%) of these total unconverted carbohydrates are present in unhydrolyzed solid (UHS) residues.
28360940	3	106	theme	carbohydrates	613:625	arg1	%					583:583	7 out of 22%	572:583	7 out of 22%	572:583	Nearly one third (7 out of 22%) of these total unconverted carbohydrates are present in unhydrolyzed solid (UHS) residues.
28360940	5	107	theme	commercial	842:851	arg1	cocktails					860:868	Current commercial enzyme cocktails	834:868	Current commercial enzyme cocktails	834:868	Current commercial enzyme cocktails are not effective to digest specific cross-linkages in plant cell wall glycans, especially some of those present in hemicelluloses and pectins.
28360940	5	108	theme	specific	898:905	arg1	cross-linkages					907:920	specific cross-linkages	898:920	specific cross-linkages in plant cell wall glycans, especially some of those present in hemicelluloses and pectins	898:1011	Current commercial enzyme cocktails are not effective to digest specific cross-linkages in plant cell wall glycans, especially some of those present in hemicelluloses and pectins.
28360940	2	109	theme	enzymes/g	534:542	arg1	biomass					544:550	20 mg enzymes/g biomass	528:550	20 mg enzymes/g biomass	528:550	Previous work has shown 22% of total carbohydrates are typically unconverted, remaining as soluble or insoluble oligomers after hydrolysis (72 h) with excess commercial enzyme loading (20 mg enzymes/g biomass).
28360940	2	109	theme	enzymes/g	534:542	arg1	loading					519:525	excess commercial enzyme loading	494:525	excess commercial enzyme loading (20 mg enzymes/g biomass)	494:551	Previous work has shown 22% of total carbohydrates are typically unconverted, remaining as soluble or insoluble oligomers after hydrolysis (72 h) with excess commercial enzyme loading (20 mg enzymes/g biomass).
28360940	12	110	theme	cell	2383:2386	arg1	carbohydrates					2393:2405	recalcitrant cell wall carbohydrates	2370:2405	recalcitrant cell wall carbohydrates	2370:2405	CONCLUSION To our knowledge, this is the first report using glycome profiling as a tool to dynamically monitor recalcitrant cell wall carbohydrates during the course of enzymatic hydrolysis.
28360940	15	111	theme	rapid	2851:2855	arg1	tool					2857:2860	a rapid tool	2849:2860	a rapid tool to assist in developing new enzyme cocktails	2849:2905	This methodology provides a rapid tool to assist in developing new enzyme cocktails, by supplementing the existing cocktails with the required enzyme activities for achieving complete deconstruction of pretreated biomass in the future.
28360940	8	112	theme	%	1595:1595	arg1	loading					1624:1630	~20% solid loading	1613:1630	~20% solid loading	1613:1630	The pretreated CS was hydrolyzed using commercial enzyme mixtures comprising cellulase and hemicellulase at 7% glucan loading (~20% solid loading).
28360940	8	112	theme	%	1595:1595	arg1	loading					1604:1610	7% glucan loading	1594:1610	7% glucan loading (~20% solid loading)	1594:1631	The pretreated CS was hydrolyzed using commercial enzyme mixtures comprising cellulase and hemicellulase at 7% glucan loading (~20% solid loading).
28360940	0	113	from	characterization	14:29	arg1	solids					102:107	unhydrolyzed solids	89:107	unhydrolyzed solids from AFEX-pretreated corn stover	89:140	Comprehensive characterization of non-cellulosic recalcitrant cell wall carbohydrates in unhydrolyzed solids from AFEX-pretreated corn stover.
28360940	14	114	theme	4-O-methyl-d-glucuronic	2621:2643	arg1	xylan					2662:2666	4-O-methyl-d-glucuronic acid-substituted xylan	2621:2666	4-O-methyl-d-glucuronic acid-substituted xylan	2621:2666	The major polysaccharides include 4-O-methyl-d-glucuronic acid-substituted xylan and pectic-arabinogalactan, suggesting that enzymes with glucuronidase and arabinofuranosidase activities are required to maximize monomeric sugar yields.
28360940	0	115	theme	corn	130:133	arg1	stover					135:140	AFEX-pretreated corn stover	114:140	AFEX-pretreated corn stover	114:140	Comprehensive characterization of non-cellulosic recalcitrant cell wall carbohydrates in unhydrolyzed solids from AFEX-pretreated corn stover.
28360940	0	116	from	carbohydrates	72:84	arg1	solids					102:107	unhydrolyzed solids	89:107	unhydrolyzed solids from AFEX-pretreated corn stover	89:140	Comprehensive characterization of non-cellulosic recalcitrant cell wall carbohydrates in unhydrolyzed solids from AFEX-pretreated corn stover.
28360940	0	116	from	carbohydrates	72:84	arg1	stover					135:140	AFEX-pretreated corn stover	114:140	AFEX-pretreated corn stover	114:140	Comprehensive characterization of non-cellulosic recalcitrant cell wall carbohydrates in unhydrolyzed solids from AFEX-pretreated corn stover.
28360940	12	117	theme	glycome	2319:2325	arg1	profiling					2327:2335	glycome profiling	2319:2335	glycome profiling	2319:2335	CONCLUSION To our knowledge, this is the first report using glycome profiling as a tool to dynamically monitor recalcitrant cell wall carbohydrates during the course of enzymatic hydrolysis.
28360940	12	117	theme	glycome	2319:2325	arg1	tool					2342:2345	a tool	2340:2345	a tool to dynamically monitor recalcitrant cell wall carbohydrates during the course of enzymatic hydrolysis	2340:2447	CONCLUSION To our knowledge, this is the first report using glycome profiling as a tool to dynamically monitor recalcitrant cell wall carbohydrates during the course of enzymatic hydrolysis.
28360940	9	118	from	hydrolysate	1678:1688	arg1	present					1652:1658	present	1652:1658	present	1652:1658	The carbohydrates present in UHS and liquid hydrolysate were evaluated over a time period of 168 h enzymatic hydrolysis.
28360940	5	119	from	cross-linkages	907:920	arg1	those					969:973	those	969:973	those	969:973	Current commercial enzyme cocktails are not effective to digest specific cross-linkages in plant cell wall glycans, especially some of those present in hemicelluloses and pectins.
28360940	5	119	from	cross-linkages	907:920	arg1	glycans					941:947	plant cell wall glycans	925:947	plant cell wall glycans	925:947	Current commercial enzyme cocktails are not effective to digest specific cross-linkages in plant cell wall glycans, especially some of those present in hemicelluloses and pectins.
28360940	5	119	from	cross-linkages	907:920	arg1	some					961:964	some	961:964	some	961:964	Current commercial enzyme cocktails are not effective to digest specific cross-linkages in plant cell wall glycans, especially some of those present in hemicelluloses and pectins.
28360940	7	120	theme	AFEX-pretreated	1376:1390	arg1	CS					1405:1406	CS	1405:1406	CS	1405:1406	RESULTS In this work, cell wall glycans that could not be enzymatically converted to monomeric sugars from AFEX-pretreated corn stover (CS) were characterized using compositional analysis and glycome profiling tools.
28360940	7	120	theme	AFEX-pretreated	1376:1390	arg1	stover					1397:1402	AFEX-pretreated corn stover	1376:1402	AFEX-pretreated corn stover (CS)	1376:1407	RESULTS In this work, cell wall glycans that could not be enzymatically converted to monomeric sugars from AFEX-pretreated corn stover (CS) were characterized using compositional analysis and glycome profiling tools.
28360940	3	121	from	present	631:637	arg1	residues					667:674	unhydrolyzed solid (UHS) residues	642:674	unhydrolyzed solid (UHS) residues	642:674	Nearly one third (7 out of 22%) of these total unconverted carbohydrates are present in unhydrolyzed solid (UHS) residues.
28360940	2	122	theme	soluble	434:440	arg1	oligomers					455:463	soluble or insoluble oligomers	434:463	soluble or insoluble oligomers	434:463	Previous work has shown 22% of total carbohydrates are typically unconverted, remaining as soluble or insoluble oligomers after hydrolysis (72 h) with excess commercial enzyme loading (20 mg enzymes/g biomass).
28360940	14	123	theme	sugar	2809:2813	arg1	yields					2815:2820	monomeric sugar yields	2799:2820	monomeric sugar yields	2799:2820	The major polysaccharides include 4-O-methyl-d-glucuronic acid-substituted xylan and pectic-arabinogalactan, suggesting that enzymes with glucuronidase and arabinofuranosidase activities are required to maximize monomeric sugar yields.
28360940	8	124	theme	~20	1613:1615	arg1	%					1616:1616	%	1616:1616	%	1616:1616	The pretreated CS was hydrolyzed using commercial enzyme mixtures comprising cellulase and hemicellulase at 7% glucan loading (~20% solid loading).
28360940	4	125	theme	considerable	736:747	arg1	loss					761:764	a considerable sugar yield loss	734:764	a considerable sugar yield loss	734:764	The presence of these unconverted carbohydrates leads to a considerable sugar yield loss, which negatively impacts the overall economics of the biorefinery.
28360940	12	126	theme	enzymatic	2428:2436	arg1	hydrolysis					2438:2447	enzymatic hydrolysis	2428:2447	enzymatic hydrolysis	2428:2447	CONCLUSION To our knowledge, this is the first report using glycome profiling as a tool to dynamically monitor recalcitrant cell wall carbohydrates during the course of enzymatic hydrolysis.
28360940	0	127	theme	non-cellulosic	34:47	arg1	carbohydrates					72:84	non-cellulosic recalcitrant cell wall carbohydrates	34:84	non-cellulosic recalcitrant cell wall carbohydrates in unhydrolyzed solids from AFEX-pretreated corn stover	34:140	Comprehensive characterization of non-cellulosic recalcitrant cell wall carbohydrates in unhydrolyzed solids from AFEX-pretreated corn stover.
28360940	9	128	from	UHS	1663:1665	arg1	present					1652:1658	present	1652:1658	present	1652:1658	The carbohydrates present in UHS and liquid hydrolysate were evaluated over a time period of 168 h enzymatic hydrolysis.
28360940	6	129	theme	glycan	1085:1090	arg1	cross-linkages					1092:1105	the most recalcitrant non-cellulosic glycan cross-linkages	1048:1105	the most recalcitrant non-cellulosic glycan cross-linkages	1048:1105	Thus, obtaining information about the most recalcitrant non-cellulosic glycan cross-linkages becomes a key study to rationally improve commercial enzyme cocktails, by supplementing the required enzyme activities for hydrolyzing those unconverted glycans.
28360940	8	130	theme	solid	1618:1622	arg1	loading					1624:1630	~20% solid loading	1613:1630	~20% solid loading	1613:1630	The pretreated CS was hydrolyzed using commercial enzyme mixtures comprising cellulase and hemicellulase at 7% glucan loading (~20% solid loading).
28360940	8	130	theme	solid	1618:1622	arg1	loading					1604:1610	7% glucan loading	1594:1610	7% glucan loading (~20% solid loading)	1594:1631	The pretreated CS was hydrolyzed using commercial enzyme mixtures comprising cellulase and hemicellulase at 7% glucan loading (~20% solid loading).
28360940	4	131	theme	yield	755:759	arg1	loss					761:764	a considerable sugar yield loss	734:764	a considerable sugar yield loss	734:764	The presence of these unconverted carbohydrates leads to a considerable sugar yield loss, which negatively impacts the overall economics of the biorefinery.
28360940	14	132	with	enzymes	2712:2718	arg1	arabinofuranosidase					2743:2761	arabinofuranosidase	2743:2761	arabinofuranosidase	2743:2761	The major polysaccharides include 4-O-methyl-d-glucuronic acid-substituted xylan and pectic-arabinogalactan, suggesting that enzymes with glucuronidase and arabinofuranosidase activities are required to maximize monomeric sugar yields.
28360940	14	132	with	enzymes	2712:2718	arg1	glucuronidase					2725:2737	glucuronidase	2725:2737	glucuronidase	2725:2737	The major polysaccharides include 4-O-methyl-d-glucuronic acid-substituted xylan and pectic-arabinogalactan, suggesting that enzymes with glucuronidase and arabinofuranosidase activities are required to maximize monomeric sugar yields.
28360940	0	133	theme	cell	62:65	arg1	carbohydrates					72:84	non-cellulosic recalcitrant cell wall carbohydrates	34:84	non-cellulosic recalcitrant cell wall carbohydrates in unhydrolyzed solids from AFEX-pretreated corn stover	34:140	Comprehensive characterization of non-cellulosic recalcitrant cell wall carbohydrates in unhydrolyzed solids from AFEX-pretreated corn stover.
28360940	13	134	theme	liquid	2479:2484	arg1	hydrolysates					2486:2497	liquid hydrolysates	2479:2497	liquid hydrolysates	2479:2497	Glycome profiling of UHS and liquid hydrolysates unveiled some of the glycans that are not cleaved and enriched after enzyme hydrolysis.
28360940	9	135	from	present	1652:1658	arg1	UHS					1663:1665	UHS	1663:1665	UHS	1663:1665	The carbohydrates present in UHS and liquid hydrolysate were evaluated over a time period of 168 h enzymatic hydrolysis.
28360940	9	135	from	present	1652:1658	arg1	hydrolysate					1678:1688	liquid hydrolysate	1671:1688	liquid hydrolysate	1671:1688	The carbohydrates present in UHS and liquid hydrolysate were evaluated over a time period of 168 h enzymatic hydrolysis.
28360940	6	136	theme	recalcitrant	1057:1068	arg1	cross-linkages					1092:1105	the most recalcitrant non-cellulosic glycan cross-linkages	1048:1105	the most recalcitrant non-cellulosic glycan cross-linkages	1048:1105	Thus, obtaining information about the most recalcitrant non-cellulosic glycan cross-linkages becomes a key study to rationally improve commercial enzyme cocktails, by supplementing the required enzyme activities for hydrolyzing those unconverted glycans.
28360940	6	137	theme	key	1117:1119	arg1	study					1121:1125	a key study	1115:1125	a key study to rationally improve commercial enzyme cocktails	1115:1175	Thus, obtaining information about the most recalcitrant non-cellulosic glycan cross-linkages becomes a key study to rationally improve commercial enzyme cocktails, by supplementing the required enzyme activities for hydrolyzing those unconverted glycans.
28360940	3	138	theme	unhydrolyzed	642:653	arg1	residues					667:674	unhydrolyzed solid (UHS) residues	642:674	unhydrolyzed solid (UHS) residues	642:674	Nearly one third (7 out of 22%) of these total unconverted carbohydrates are present in unhydrolyzed solid (UHS) residues.
28360940	15	139	from	deconstruction	3007:3020	arg1	future					3051:3056	future	3051:3056	future	3051:3056	This methodology provides a rapid tool to assist in developing new enzyme cocktails, by supplementing the existing cocktails with the required enzyme activities for achieving complete deconstruction of pretreated biomass in the future.
28360940	2	140	theme	insoluble	445:453	arg1	oligomers					455:463	soluble or insoluble oligomers	434:463	soluble or insoluble oligomers	434:463	Previous work has shown 22% of total carbohydrates are typically unconverted, remaining as soluble or insoluble oligomers after hydrolysis (72 h) with excess commercial enzyme loading (20 mg enzymes/g biomass).
28360940	0	141	theme	carbohydrates	72:84	arg1	characterization					14:29	Comprehensive characterization	0:29	Comprehensive characterization of non-cellulosic recalcitrant cell wall carbohydrates in unhydrolyzed solids from AFEX-pretreated corn stover.	0:141	Comprehensive characterization of non-cellulosic recalcitrant cell wall carbohydrates in unhydrolyzed solids from AFEX-pretreated corn stover.
28360940	2	142	theme	excess	494:499	arg1	biomass					544:550	20 mg enzymes/g biomass	528:550	20 mg enzymes/g biomass	528:550	Previous work has shown 22% of total carbohydrates are typically unconverted, remaining as soluble or insoluble oligomers after hydrolysis (72 h) with excess commercial enzyme loading (20 mg enzymes/g biomass).
28360940	2	142	theme	excess	494:499	arg1	loading					519:525	excess commercial enzyme loading	494:525	excess commercial enzyme loading (20 mg enzymes/g biomass)	494:551	Previous work has shown 22% of total carbohydrates are typically unconverted, remaining as soluble or insoluble oligomers after hydrolysis (72 h) with excess commercial enzyme loading (20 mg enzymes/g biomass).
28360940	2	143	theme	total	374:378	arg1	carbohydrates					380:392	total carbohydrates	374:392	total carbohydrates	374:392	Previous work has shown 22% of total carbohydrates are typically unconverted, remaining as soluble or insoluble oligomers after hydrolysis (72 h) with excess commercial enzyme loading (20 mg enzymes/g biomass).
28360940	14	144	theme	acid-substituted	2645:2660	arg1	xylan					2662:2666	4-O-methyl-d-glucuronic acid-substituted xylan	2621:2666	4-O-methyl-d-glucuronic acid-substituted xylan	2621:2666	The major polysaccharides include 4-O-methyl-d-glucuronic acid-substituted xylan and pectic-arabinogalactan, suggesting that enzymes with glucuronidase and arabinofuranosidase activities are required to maximize monomeric sugar yields.
28360940	6	145	theme	enzyme	1160:1165	arg1	cocktails					1167:1175	commercial enzyme cocktails	1149:1175	commercial enzyme cocktails	1149:1175	Thus, obtaining information about the most recalcitrant non-cellulosic glycan cross-linkages becomes a key study to rationally improve commercial enzyme cocktails, by supplementing the required enzyme activities for hydrolyzing those unconverted glycans.
28360940	10	146	theme	hydrolysis	1937:1946	arg1	course					1917:1922	the course	1913:1922	the course of enzymatic hydrolysis	1913:1946	Cell wall glycan-specific monoclonal antibodies (mAbs) were used to characterize the type and abundance of non-cellulosic polysaccharides present in UHS over the course of enzymatic hydrolysis.
28360940	13	147	theme	UHS	2471:2473	arg1	profiling					2458:2466	Glycome profiling	2450:2466	Glycome profiling of UHS and liquid hydrolysates	2450:2497	Glycome profiling of UHS and liquid hydrolysates unveiled some of the glycans that are not cleaved and enriched after enzyme hydrolysis.
28360940	7	148	theme	wall	1296:1299	arg1	glycans					1301:1307	cell wall glycans	1291:1307	cell wall glycans that could not be enzymatically converted to monomeric sugars from AFEX-pretreated corn stover (CS)	1291:1407	RESULTS In this work, cell wall glycans that could not be enzymatically converted to monomeric sugars from AFEX-pretreated corn stover (CS) were characterized using compositional analysis and glycome profiling tools.
28360940	14	149	dep	glucuronidase	2725:2737	arg1	activities					2763:2772	activities	2763:2772	activities	2763:2772	The major polysaccharides include 4-O-methyl-d-glucuronic acid-substituted xylan and pectic-arabinogalactan, suggesting that enzymes with glucuronidase and arabinofuranosidase activities are required to maximize monomeric sugar yields.
28360940	3	150	from	residues	667:674	arg1	present					631:637	present	631:637	present	631:637	Nearly one third (7 out of 22%) of these total unconverted carbohydrates are present in unhydrolyzed solid (UHS) residues.
28360940	10	151	theme	non-cellulosic	1862:1875	arg1	polysaccharides					1877:1891	non-cellulosic polysaccharides	1862:1891	non-cellulosic polysaccharides present in UHS	1862:1906	Cell wall glycan-specific monoclonal antibodies (mAbs) were used to characterize the type and abundance of non-cellulosic polysaccharides present in UHS over the course of enzymatic hydrolysis.
28360940	11	152	theme	4-O-methyl-d-glucuronic	1949:1971	arg1	pectic-arabinogalactan					2000:2021	pectic-arabinogalactan	2000:2021	pectic-arabinogalactan	2000:2021	4-O-methyl-d-glucuronic acid-substituted xylan and pectic-arabinogalactan were found to be the most abundant epitopes recognized by mAbs in UHS and liquid hydrolysate, suggesting that the commercial enzyme cocktails used in this work are unable to effectively target those substituted polysaccharide residues.
28360940	11	152	theme	4-O-methyl-d-glucuronic	1949:1971	arg1	epitopes					2058:2065	the most abundant epitopes	2040:2065	the most abundant epitopes recognized by mAbs in UHS and liquid hydrolysate	2040:2114	4-O-methyl-d-glucuronic acid-substituted xylan and pectic-arabinogalactan were found to be the most abundant epitopes recognized by mAbs in UHS and liquid hydrolysate, suggesting that the commercial enzyme cocktails used in this work are unable to effectively target those substituted polysaccharide residues.
28360940	11	152	theme	4-O-methyl-d-glucuronic	1949:1971	arg1	xylan					1990:1994	4-O-methyl-d-glucuronic acid-substituted xylan	1949:1994	4-O-methyl-d-glucuronic acid-substituted xylan	1949:1994	4-O-methyl-d-glucuronic acid-substituted xylan and pectic-arabinogalactan were found to be the most abundant epitopes recognized by mAbs in UHS and liquid hydrolysate, suggesting that the commercial enzyme cocktails used in this work are unable to effectively target those substituted polysaccharide residues.
28360940	5	153	from	hemicelluloses	986:999	arg1	present					975:981	present	975:981	present	975:981	Current commercial enzyme cocktails are not effective to digest specific cross-linkages in plant cell wall glycans, especially some of those present in hemicelluloses and pectins.
28360940	1	154	theme	dilute	298:303	arg1	acid					305:308	dilute acid	298:308	dilute acid	298:308	BACKGROUND Inefficient carbohydrate conversion has been an unsolved problem for various lignocellulosic biomass pretreatment technologies, including AFEX, dilute acid, and ionic liquid pretreatments.
24945580	11	0	theme	pseudo-second-order	1421:1439	arg1	model					1441:1445	the pseudo-second-order model	1417:1445	the pseudo-second-order model	1417:1445	When the amount of composite beads was varied, the results of kinetic studies of malachite green adsorption by immobilized cells of P. sanguineus fitted well with the pseudo-second-order model.
24945580	12	1	theme	composite	1488:1496	arg1	beads					1498:1502	the magnetic composite beads	1475:1502	the magnetic composite beads effectively adsorbed the dye solution from wastewater	1475:1556	The results indicated that the magnetic composite beads effectively adsorbed the dye solution from wastewater and were environmentally friendly.
24945580	12	1	theme	composite	1488:1496	arg1	friendly					1583:1590	environmentally friendly	1567:1590	environmentally friendly	1567:1590	The results indicated that the magnetic composite beads effectively adsorbed the dye solution from wastewater and were environmentally friendly.
24945580	8	2	theme	alginate	867:874	arg1	composites					876:885	alginate composites	867:885	alginate composites	867:885	The magnetic guidability of alginate composites and biocompatibility of iron oxide nanoparticles facilitated the magnetic filtration and separation processes.
24945580	7	3	theme	oxide	747:751	arg1	nanoparticles					753:765	iron oxide nanoparticles	742:765	iron oxide nanoparticles	742:765	Superparamagnetic nanomaterials, iron oxide nanoparticles, were combined with alginate gels to form magnetic alginate composites.
24945580	7	3	theme	oxide	747:751	arg1	nanomaterials					727:739	Superparamagnetic nanomaterials	709:739	Superparamagnetic nanomaterials	709:739	Superparamagnetic nanomaterials, iron oxide nanoparticles, were combined with alginate gels to form magnetic alginate composites.
24945580	0	4	theme	aqueous	85:91	arg1	solutions					93:101	aqueous solutions	85:101	aqueous solutions	85:101	Magnetic Pycnoporus sanguineus-loaded alginate composite beads for removing dye from aqueous solutions.
24945580	11	5	theme	malachite	1335:1343	arg1	green					1345:1349	malachite green	1335:1349	malachite green adsorption by immobilized cells of P. sanguineus	1335:1398	When the amount of composite beads was varied, the results of kinetic studies of malachite green adsorption by immobilized cells of P. sanguineus fitted well with the pseudo-second-order model.
24945580	12	6	theme	dye	1529:1531	arg1	solution					1533:1540	the dye solution	1525:1540	the dye solution	1525:1540	The results indicated that the magnetic composite beads effectively adsorbed the dye solution from wastewater and were environmentally friendly.
24945580	11	7	theme	kinetic	1316:1322	arg1	studies					1324:1330	kinetic studies	1316:1330	kinetic studies of malachite green adsorption by immobilized cells of P. sanguineus	1316:1398	When the amount of composite beads was varied, the results of kinetic studies of malachite green adsorption by immobilized cells of P. sanguineus fitted well with the pseudo-second-order model.
24945580	2	8	used	used	310:313	arg2	process					299:305	A conventional process	284:305	A conventional process	284:305	A conventional process is used to adsorb dyes and filter wastewater.
24945580	3	9	theme	emerging	379:386	arg1	technology					388:397	an emerging technology	376:397	an emerging technology	376:397	Magnetic filtration is an emerging technology.
24945580	3	9	theme	emerging	379:386	arg1	filtration					362:371	Magnetic filtration	353:371	Magnetic filtration	353:371	Magnetic filtration is an emerging technology.
24945580	4	10	theme	dye	504:506	arg1	solution					508:515	a dye solution	502:515	a dye solution	502:515	In this study, magnetic Pycnoporus sanguineus-loaded alginate composite beads were employed to remove a dye solution.
24945580	11	11	theme	studies	1324:1330	arg1	results					1305:1311	the results	1301:1311	the results of kinetic studies of malachite green adsorption by immobilized cells of P. sanguineus	1301:1398	When the amount of composite beads was varied, the results of kinetic studies of malachite green adsorption by immobilized cells of P. sanguineus fitted well with the pseudo-second-order model.
24945580	4	12	theme	Pycnoporus	424:433	arg1	beads					472:476	magnetic Pycnoporus sanguineus-loaded alginate composite beads	415:476	magnetic Pycnoporus sanguineus-loaded alginate composite beads	415:476	In this study, magnetic Pycnoporus sanguineus-loaded alginate composite beads were employed to remove a dye solution.
24945580	8	13	theme	iron	911:914	arg1	nanoparticles					922:934	iron oxide nanoparticles	911:934	iron oxide nanoparticles	911:934	The magnetic guidability of alginate composites and biocompatibility of iron oxide nanoparticles facilitated the magnetic filtration and separation processes.
24945580	2	14	dep	dyes	325:328	arg1	wastewater					341:350	wastewater	341:350	wastewater	341:350	A conventional process is used to adsorb dyes and filter wastewater.
24945580	12	15	theme	magnetic	1479:1486	arg1	beads					1498:1502	the magnetic composite beads	1475:1502	the magnetic composite beads effectively adsorbed the dye solution from wastewater	1475:1556	The results indicated that the magnetic composite beads effectively adsorbed the dye solution from wastewater and were environmentally friendly.
24945580	12	15	theme	magnetic	1479:1486	arg1	friendly					1583:1590	environmentally friendly	1567:1590	environmentally friendly	1567:1590	The results indicated that the magnetic composite beads effectively adsorbed the dye solution from wastewater and were environmentally friendly.
24945580	10	16	theme	composite	1158:1166	arg1	beads					1168:1172	The composite beads	1154:1172	The composite beads	1154:1172	The composite beads could be removed easily post-adsorption by using a magnetic filtration process.
24945580	5	17	theme	dye	623:625	arg1	solution					627:634	the dye solution	619:634	the dye solution	619:634	A white rot fungus, P. sanguineus, immobilized in alginate beads were used as a biosorbent to remove the dye solution.
24945580	4	18	theme	magnetic	415:422	arg1	beads					472:476	magnetic Pycnoporus sanguineus-loaded alginate composite beads	415:476	magnetic Pycnoporus sanguineus-loaded alginate composite beads	415:476	In this study, magnetic Pycnoporus sanguineus-loaded alginate composite beads were employed to remove a dye solution.
24945580	5	19	theme	white	520:524	arg1	sanguineus					541:550	P. sanguineus	538:550	P. sanguineus	538:550	A white rot fungus, P. sanguineus, immobilized in alginate beads were used as a biosorbent to remove the dye solution.
24945580	5	19	theme	white	520:524	arg1	biosorbent					598:607	a biosorbent	596:607	a biosorbent	596:607	A white rot fungus, P. sanguineus, immobilized in alginate beads were used as a biosorbent to remove the dye solution.
24945580	5	19	theme	white	520:524	arg1	fungus					530:535	A white rot fungus	518:535	A white rot fungus	518:535	A white rot fungus, P. sanguineus, immobilized in alginate beads were used as a biosorbent to remove the dye solution.
24945580	7	20	theme	iron	742:745	arg1	nanoparticles					753:765	iron oxide nanoparticles	742:765	iron oxide nanoparticles	742:765	Superparamagnetic nanomaterials, iron oxide nanoparticles, were combined with alginate gels to form magnetic alginate composites.
24945580	7	20	theme	iron	742:745	arg1	nanomaterials					727:739	Superparamagnetic nanomaterials	709:739	Superparamagnetic nanomaterials	709:739	Superparamagnetic nanomaterials, iron oxide nanoparticles, were combined with alginate gels to form magnetic alginate composites.
24945580	8	21	theme	magnetic	843:850	arg1	guidability					852:862	The magnetic guidability	839:862	The magnetic guidability of alginate composites	839:885	The magnetic guidability of alginate composites and biocompatibility of iron oxide nanoparticles facilitated the magnetic filtration and separation processes.
24945580	2	22	theme	conventional	286:297	arg1	process					299:305	A conventional process	284:305	A conventional process	284:305	A conventional process is used to adsorb dyes and filter wastewater.
24945580	5	23	used	used	588:591	arg2	fungus					530:535	A white rot fungus	518:535	A white rot fungus	518:535	A white rot fungus, P. sanguineus, immobilized in alginate beads were used as a biosorbent to remove the dye solution.
24945580	5	23	used	used	588:591	arg2	biosorbent					598:607	a biosorbent	596:607	a biosorbent	596:607	A white rot fungus, P. sanguineus, immobilized in alginate beads were used as a biosorbent to remove the dye solution.
24945580	5	23	used	used	588:591	arg2	sanguineus					541:550	P. sanguineus	538:550	P. sanguineus	538:550	A white rot fungus, P. sanguineus, immobilized in alginate beads were used as a biosorbent to remove the dye solution.
24945580	8	24	theme	nanoparticles	922:934	arg1	guidability					852:862	The magnetic guidability	839:862	The magnetic guidability of alginate composites	839:885	The magnetic guidability of alginate composites and biocompatibility of iron oxide nanoparticles facilitated the magnetic filtration and separation processes.
24945580	8	24	theme	nanoparticles	922:934	arg1	biocompatibility					891:906	biocompatibility	891:906	biocompatibility of iron oxide nanoparticles	891:934	The magnetic guidability of alginate composites and biocompatibility of iron oxide nanoparticles facilitated the magnetic filtration and separation processes.
24945580	11	25	theme	green	1345:1349	arg1	adsorption					1351:1360	malachite green adsorption	1335:1360	malachite green adsorption by immobilized cells of P. sanguineus	1335:1398	When the amount of composite beads was varied, the results of kinetic studies of malachite green adsorption by immobilized cells of P. sanguineus fitted well with the pseudo-second-order model.
24945580	11	26	theme	adsorption	1351:1360	arg1	studies					1324:1330	kinetic studies	1316:1330	kinetic studies of malachite green adsorption by immobilized cells of P. sanguineus	1316:1398	When the amount of composite beads was varied, the results of kinetic studies of malachite green adsorption by immobilized cells of P. sanguineus fitted well with the pseudo-second-order model.
24945580	8	27	theme	oxide	916:920	arg1	nanoparticles					922:934	iron oxide nanoparticles	911:934	iron oxide nanoparticles	911:934	The magnetic guidability of alginate composites and biocompatibility of iron oxide nanoparticles facilitated the magnetic filtration and separation processes.
24945580	9	28	theme	concentration	1104:1116	arg1	influence					1075:1083	the influence	1071:1083	the influence of the initial dye concentration and pH on the biosorption capacity	1071:1151	The fungus cells were immobilized in loaded alginate composites to study the influence of the initial dye concentration and pH on the biosorption capacity.
24945580	0	29	theme	Magnetic	0:7	arg1	Pycnoporus					9:18	Magnetic Pycnoporus	0:18	Magnetic Pycnoporus	0:18	Magnetic Pycnoporus sanguineus-loaded alginate composite beads for removing dye from aqueous solutions.
24945580	9	30	theme	biosorption	1132:1142	arg1	capacity					1144:1151	the biosorption capacity	1128:1151	the biosorption capacity	1128:1151	The fungus cells were immobilized in loaded alginate composites to study the influence of the initial dye concentration and pH on the biosorption capacity.
24945580	11	31	theme	sanguineus	1389:1398	arg1	adsorption					1351:1360	malachite green adsorption	1335:1360	malachite green adsorption by immobilized cells of P. sanguineus	1335:1398	When the amount of composite beads was varied, the results of kinetic studies of malachite green adsorption by immobilized cells of P. sanguineus fitted well with the pseudo-second-order model.
24945580	1	32	theme	Dye	104:106	arg1	problem					158:164	a severe environmental problem	135:164	a severe environmental problem because treating water containing dyes using conventional physical, chemical, and biological treatments is difficult	135:281	Dye pollution in wastewater is a severe environmental problem because treating water containing dyes using conventional physical, chemical, and biological treatments is difficult.
24945580	1	32	theme	Dye	104:106	arg1	pollution					108:116	Dye pollution	104:116	Dye pollution in wastewater	104:130	Dye pollution in wastewater is a severe environmental problem because treating water containing dyes using conventional physical, chemical, and biological treatments is difficult.
24945580	6	33	theme	acidic	688:693	arg1	environments					695:706	acidic environments	688:706	acidic environments	688:706	An alginate polymer could protect P. sanguineus in acidic environments.
24945580	0	34	theme	alginate	38:45	arg1	beads					57:61	alginate composite beads	38:61	alginate composite beads	38:61	Magnetic Pycnoporus sanguineus-loaded alginate composite beads for removing dye from aqueous solutions.
24945580	9	35	from	influence	1075:1083	arg1	capacity					1144:1151	the biosorption capacity	1128:1151	the biosorption capacity	1128:1151	The fungus cells were immobilized in loaded alginate composites to study the influence of the initial dye concentration and pH on the biosorption capacity.
24945580	10	36	theme	filtration	1234:1243	arg1	process					1245:1251	a magnetic filtration process	1223:1251	a magnetic filtration process	1223:1251	The composite beads could be removed easily post-adsorption by using a magnetic filtration process.
24945580	1	37	theme	conventional	211:222	arg1	treatments					259:268	conventional physical, chemical, and biological treatments	211:268	conventional physical, chemical, and biological treatments	211:268	Dye pollution in wastewater is a severe environmental problem because treating water containing dyes using conventional physical, chemical, and biological treatments is difficult.
24945580	9	38	theme	alginate	1042:1049	arg1	composites					1051:1060	loaded alginate composites	1035:1060	loaded alginate composites	1035:1060	The fungus cells were immobilized in loaded alginate composites to study the influence of the initial dye concentration and pH on the biosorption capacity.
24945580	7	39	theme	magnetic	809:816	arg1	composites					827:836	magnetic alginate composites	809:836	magnetic alginate composites	809:836	Superparamagnetic nanomaterials, iron oxide nanoparticles, were combined with alginate gels to form magnetic alginate composites.
24945580	7	40	theme	Superparamagnetic	709:725	arg1	nanoparticles					753:765	iron oxide nanoparticles	742:765	iron oxide nanoparticles	742:765	Superparamagnetic nanomaterials, iron oxide nanoparticles, were combined with alginate gels to form magnetic alginate composites.
24945580	7	40	theme	Superparamagnetic	709:725	arg1	nanomaterials					727:739	Superparamagnetic nanomaterials	709:739	Superparamagnetic nanomaterials	709:739	Superparamagnetic nanomaterials, iron oxide nanoparticles, were combined with alginate gels to form magnetic alginate composites.
24945580	1	41	dep	problem	158:164	arg1	difficult					273:281	difficult	273:281	difficult	273:281	Dye pollution in wastewater is a severe environmental problem because treating water containing dyes using conventional physical, chemical, and biological treatments is difficult.
24945580	1	42	theme	physical	224:231	arg1	treatments					259:268	conventional physical, chemical, and biological treatments	211:268	conventional physical, chemical, and biological treatments	211:268	Dye pollution in wastewater is a severe environmental problem because treating water containing dyes using conventional physical, chemical, and biological treatments is difficult.
24945580	7	43	theme	alginate	818:825	arg1	composites					827:836	magnetic alginate composites	809:836	magnetic alginate composites	809:836	Superparamagnetic nanomaterials, iron oxide nanoparticles, were combined with alginate gels to form magnetic alginate composites.
24945580	11	44	theme	composite	1273:1281	arg1	beads					1283:1287	composite beads	1273:1287	composite beads	1273:1287	When the amount of composite beads was varied, the results of kinetic studies of malachite green adsorption by immobilized cells of P. sanguineus fitted well with the pseudo-second-order model.
24945580	12	45	theme	environmentally	1567:1581	arg1	beads					1498:1502	the magnetic composite beads	1475:1502	the magnetic composite beads effectively adsorbed the dye solution from wastewater	1475:1556	The results indicated that the magnetic composite beads effectively adsorbed the dye solution from wastewater and were environmentally friendly.
24945580	12	45	theme	environmentally	1567:1581	arg1	friendly					1583:1590	environmentally friendly	1567:1590	environmentally friendly	1567:1590	The results indicated that the magnetic composite beads effectively adsorbed the dye solution from wastewater and were environmentally friendly.
24945580	8	46	theme	magnetic	952:959	arg1	filtration					961:970	the magnetic filtration	948:970	the magnetic filtration	948:970	The magnetic guidability of alginate composites and biocompatibility of iron oxide nanoparticles facilitated the magnetic filtration and separation processes.
24945580	0	47	theme	composite	47:55	arg1	beads					57:61	alginate composite beads	38:61	alginate composite beads	38:61	Magnetic Pycnoporus sanguineus-loaded alginate composite beads for removing dye from aqueous solutions.
24945580	12	48	dep	solution	1533:1540	arg1	wastewater					1547:1556	wastewater	1547:1556	wastewater	1547:1556	The results indicated that the magnetic composite beads effectively adsorbed the dye solution from wastewater and were environmentally friendly.
24945580	11	49	theme	beads	1283:1287	arg1	varied					1293:1298	varied	1293:1298	varied	1293:1298	When the amount of composite beads was varied, the results of kinetic studies of malachite green adsorption by immobilized cells of P. sanguineus fitted well with the pseudo-second-order model.
24945580	11	49	theme	beads	1283:1287	arg1	amount					1263:1268	the amount	1259:1268	the amount of composite beads	1259:1287	When the amount of composite beads was varied, the results of kinetic studies of malachite green adsorption by immobilized cells of P. sanguineus fitted well with the pseudo-second-order model.
24945580	11	49	theme	beads	1283:1287	arg1	beads					1283:1287	composite beads	1273:1287	composite beads	1273:1287	When the amount of composite beads was varied, the results of kinetic studies of malachite green adsorption by immobilized cells of P. sanguineus fitted well with the pseudo-second-order model.
24945580	1	50	contain	containing	189:198	arg1	water					183:187	water	183:187	water containing dyes using conventional physical, chemical, and biological treatments	183:268	Dye pollution in wastewater is a severe environmental problem because treating water containing dyes using conventional physical, chemical, and biological treatments is difficult.
24945580	1	50	contain	containing	189:198	arg2	dyes					200:203	dyes	200:203	dyes using conventional physical, chemical, and biological treatments	200:268	Dye pollution in wastewater is a severe environmental problem because treating water containing dyes using conventional physical, chemical, and biological treatments is difficult.
24945580	9	51	theme	loaded	1035:1040	arg1	composites					1051:1060	loaded alginate composites	1035:1060	loaded alginate composites	1035:1060	The fungus cells were immobilized in loaded alginate composites to study the influence of the initial dye concentration and pH on the biosorption capacity.
24945580	8	52	theme	separation	976:985	arg1	processes					987:995	separation processes	976:995	separation processes	976:995	The magnetic guidability of alginate composites and biocompatibility of iron oxide nanoparticles facilitated the magnetic filtration and separation processes.
24945580	1	53	theme	chemical	234:241	arg1	treatments					259:268	conventional physical, chemical, and biological treatments	211:268	conventional physical, chemical, and biological treatments	211:268	Dye pollution in wastewater is a severe environmental problem because treating water containing dyes using conventional physical, chemical, and biological treatments is difficult.
24945580	10	54	theme	magnetic	1225:1232	arg1	process					1245:1251	a magnetic filtration process	1223:1251	a magnetic filtration process	1223:1251	The composite beads could be removed easily post-adsorption by using a magnetic filtration process.
24945580	7	55	theme	alginate	787:794	arg1	gels					796:799	alginate gels	787:799	alginate gels	787:799	Superparamagnetic nanomaterials, iron oxide nanoparticles, were combined with alginate gels to form magnetic alginate composites.
24945580	4	56	theme	alginate	453:460	arg1	beads					472:476	magnetic Pycnoporus sanguineus-loaded alginate composite beads	415:476	magnetic Pycnoporus sanguineus-loaded alginate composite beads	415:476	In this study, magnetic Pycnoporus sanguineus-loaded alginate composite beads were employed to remove a dye solution.
24945580	11	57	theme	immobilized	1365:1375	arg1	cells					1377:1381	immobilized cells	1365:1381	immobilized cells	1365:1381	When the amount of composite beads was varied, the results of kinetic studies of malachite green adsorption by immobilized cells of P. sanguineus fitted well with the pseudo-second-order model.
24945580	5	58	theme	alginate	568:575	arg1	beads					577:581	alginate beads	568:581	alginate beads	568:581	A white rot fungus, P. sanguineus, immobilized in alginate beads were used as a biosorbent to remove the dye solution.
24945580	1	59	from	pollution	108:116	arg1	wastewater					121:130	wastewater	121:130	wastewater	121:130	Dye pollution in wastewater is a severe environmental problem because treating water containing dyes using conventional physical, chemical, and biological treatments is difficult.
24945580	9	60	theme	pH	1122:1123	arg1	influence					1075:1083	the influence	1071:1083	the influence of the initial dye concentration and pH on the biosorption capacity	1071:1151	The fungus cells were immobilized in loaded alginate composites to study the influence of the initial dye concentration and pH on the biosorption capacity.
24945580	4	61	theme	sanguineus-loaded	435:451	arg1	beads					472:476	magnetic Pycnoporus sanguineus-loaded alginate composite beads	415:476	magnetic Pycnoporus sanguineus-loaded alginate composite beads	415:476	In this study, magnetic Pycnoporus sanguineus-loaded alginate composite beads were employed to remove a dye solution.
24945580	1	62	theme	severe	137:142	arg1	problem					158:164	a severe environmental problem	135:164	a severe environmental problem because treating water containing dyes using conventional physical, chemical, and biological treatments is difficult	135:281	Dye pollution in wastewater is a severe environmental problem because treating water containing dyes using conventional physical, chemical, and biological treatments is difficult.
24945580	1	62	theme	severe	137:142	arg1	pollution					108:116	Dye pollution	104:116	Dye pollution in wastewater	104:130	Dye pollution in wastewater is a severe environmental problem because treating water containing dyes using conventional physical, chemical, and biological treatments is difficult.
24945580	5	63	theme	rot	526:528	arg1	sanguineus					541:550	P. sanguineus	538:550	P. sanguineus	538:550	A white rot fungus, P. sanguineus, immobilized in alginate beads were used as a biosorbent to remove the dye solution.
24945580	5	63	theme	rot	526:528	arg1	biosorbent					598:607	a biosorbent	596:607	a biosorbent	596:607	A white rot fungus, P. sanguineus, immobilized in alginate beads were used as a biosorbent to remove the dye solution.
24945580	5	63	theme	rot	526:528	arg1	fungus					530:535	A white rot fungus	518:535	A white rot fungus	518:535	A white rot fungus, P. sanguineus, immobilized in alginate beads were used as a biosorbent to remove the dye solution.
24945580	4	64	theme	composite	462:470	arg1	beads					472:476	magnetic Pycnoporus sanguineus-loaded alginate composite beads	415:476	magnetic Pycnoporus sanguineus-loaded alginate composite beads	415:476	In this study, magnetic Pycnoporus sanguineus-loaded alginate composite beads were employed to remove a dye solution.
24945580	9	65	theme	dye	1100:1102	arg1	concentration					1104:1116	the initial dye concentration	1088:1116	the initial dye concentration	1088:1116	The fungus cells were immobilized in loaded alginate composites to study the influence of the initial dye concentration and pH on the biosorption capacity.
24945580	1	66	theme	environmental	144:156	arg1	problem					158:164	a severe environmental problem	135:164	a severe environmental problem because treating water containing dyes using conventional physical, chemical, and biological treatments is difficult	135:281	Dye pollution in wastewater is a severe environmental problem because treating water containing dyes using conventional physical, chemical, and biological treatments is difficult.
24945580	1	66	theme	environmental	144:156	arg1	pollution					108:116	Dye pollution	104:116	Dye pollution in wastewater	104:130	Dye pollution in wastewater is a severe environmental problem because treating water containing dyes using conventional physical, chemical, and biological treatments is difficult.
24945580	6	67	theme	alginate	640:647	arg1	polymer					649:655	An alginate polymer	637:655	An alginate polymer	637:655	An alginate polymer could protect P. sanguineus in acidic environments.
24945580	1	68	theme	biological	248:257	arg1	treatments					259:268	conventional physical, chemical, and biological treatments	211:268	conventional physical, chemical, and biological treatments	211:268	Dye pollution in wastewater is a severe environmental problem because treating water containing dyes using conventional physical, chemical, and biological treatments is difficult.
24945580	3	69	theme	Magnetic	353:360	arg1	technology					388:397	an emerging technology	376:397	an emerging technology	376:397	Magnetic filtration is an emerging technology.
24945580	3	69	theme	Magnetic	353:360	arg1	filtration					362:371	Magnetic filtration	353:371	Magnetic filtration	353:371	Magnetic filtration is an emerging technology.
24945580	9	70	theme	initial	1092:1098	arg1	concentration					1104:1116	the initial dye concentration	1088:1116	the initial dye concentration	1088:1116	The fungus cells were immobilized in loaded alginate composites to study the influence of the initial dye concentration and pH on the biosorption capacity.
24945580	8	71	theme	composites	876:885	arg1	guidability					852:862	The magnetic guidability	839:862	The magnetic guidability of alginate composites	839:885	The magnetic guidability of alginate composites and biocompatibility of iron oxide nanoparticles facilitated the magnetic filtration and separation processes.
24945580	8	71	theme	composites	876:885	arg1	biocompatibility					891:906	biocompatibility	891:906	biocompatibility of iron oxide nanoparticles	891:934	The magnetic guidability of alginate composites and biocompatibility of iron oxide nanoparticles facilitated the magnetic filtration and separation processes.
24945580	9	72	theme	fungus	1002:1007	arg1	cells					1009:1013	The fungus cells	998:1013	The fungus cells	998:1013	The fungus cells were immobilized in loaded alginate composites to study the influence of the initial dye concentration and pH on the biosorption capacity.
28890371	11	0	theme	tissue	1401:1406	arg1	engineering					1408:1418	tissue engineering	1401:1418	tissue engineering	1401:1418	The obtained materials can provide the support required in tissue engineering and regenerative medicine.
28890371	6	1	theme	starch	931:936	arg1	addition					938:945	dialdehyde starch addition	920:945	dialdehyde starch addition	920:945	The results showed that the properties of the scaffolds based on chitosan, collagen, and hyaluronic acid can be modified by dialdehyde starch addition.
28890371	5	2	dep	adhesion	693:700	arg1	the					689:691	the	689:691	the	689:691	In addition, the adhesion and proliferation of human osteosarcoma SaOS-2 cells were examined on the obtained scaffolds.
28890371	7	3	theme	maximum	1004:1010	arg1	force					1024:1028	maximum compressive force	1004:1028	maximum compressive force	1004:1028	The mechanical parameters (i.e. compressive modulus and maximum compressive force), porosity, and density of the material were improved.
28890371	0	4	dep	in	137:138	arg1	situ					140:143	situ	140:143	situ	140:143	The application of chitosan/collagen/hyaluronic acid sponge cross-linked by dialdehyde starch addition as a matrix for calcium phosphate in situ precipitation.
28890371	10	5	theme	compound	1271:1278	arg1	precipitation					1280:1292	an inorganic compound precipitation	1258:1292	an inorganic compound precipitation	1258:1292	The cross-linker addition and an inorganic compound precipitation improved the biocompatibility of the scaffolds.
28890371	5	6	theme	osteosarcoma	729:740	arg1	cells					749:753	human osteosarcoma SaOS-2 cells	723:753	human osteosarcoma SaOS-2 cells	723:753	In addition, the adhesion and proliferation of human osteosarcoma SaOS-2 cells were examined on the obtained scaffolds.
28890371	6	7	theme	dialdehyde	920:929	arg1	addition					938:945	dialdehyde starch addition	920:945	dialdehyde starch addition	920:945	The results showed that the properties of the scaffolds based on chitosan, collagen, and hyaluronic acid can be modified by dialdehyde starch addition.
28890371	6	8	theme	scaffolds	842:850	arg1	properties					824:833	the properties	820:833	the properties of the scaffolds based on chitosan, collagen, and hyaluronic acid	820:899	The results showed that the properties of the scaffolds based on chitosan, collagen, and hyaluronic acid can be modified by dialdehyde starch addition.
28890371	2	9	theme	in	367:368	arg1	precipitation					375:387	calcium phosphate in situ precipitation	349:387	calcium phosphate in situ precipitation	349:387	The porous structures were used as matrixes for calcium phosphate in situ precipitation.
28890371	10	10	theme	inorganic	1261:1269	arg1	precipitation					1280:1292	an inorganic compound precipitation	1258:1292	an inorganic compound precipitation	1258:1292	The cross-linker addition and an inorganic compound precipitation improved the biocompatibility of the scaffolds.
28890371	8	11	theme	Ca/P	1141:1144	arg1	ratio					1146:1150	the Ca/P ratio ∼2	1137:1153	the Ca/P ratio ∼2	1137:1153	Calcium phosphate was deposited in the scaffolds at the Ca/P ratio ∼2.
28890371	9	12	theme	interconnected	1206:1219	arg1	pores					1221:1225	interconnected pores	1206:1225	interconnected pores	1206:1225	SEM images showed the homogeneous structure, with interconnected pores.
28890371	9	13	theme	homogeneous	1178:1188	arg1	structure					1190:1198	the homogeneous structure	1174:1198	the homogeneous structure	1174:1198	SEM images showed the homogeneous structure, with interconnected pores.
28890371	2	14	theme	porous	305:310	arg1	matrixes					336:343	matrixes	336:343	matrixes for calcium phosphate in situ precipitation	336:387	The porous structures were used as matrixes for calcium phosphate in situ precipitation.
28890371	2	14	theme	porous	305:310	arg1	structures					312:321	The porous structures	301:321	The porous structures	301:321	The porous structures were used as matrixes for calcium phosphate in situ precipitation.
28890371	7	15	dep	parameters	963:972	arg1	force					1024:1028	maximum compressive force	1004:1028	maximum compressive force	1004:1028	The mechanical parameters (i.e. compressive modulus and maximum compressive force), porosity, and density of the material were improved.
28890371	7	15	dep	parameters	963:972	arg1	modulus					992:998	compressive modulus	980:998	compressive modulus	980:998	The mechanical parameters (i.e. compressive modulus and maximum compressive force), porosity, and density of the material were improved.
28890371	2	16	dep	in	367:368	arg1	situ					370:373	situ	370:373	situ	370:373	The porous structures were used as matrixes for calcium phosphate in situ precipitation.
28890371	1	17	theme	freeze-drying	279:291	arg1	method					293:298	the freeze-drying method	275:298	the freeze-drying method	275:298	Scaffolds based on chitosan, collagen and hyaluronic acid, cross-linked by dialdehyde starch were obtained through the freeze-drying method.
28890371	0	18	theme	calcium	119:125	arg1	precipitation					145:157	calcium phosphate in situ precipitation	119:157	calcium phosphate in situ precipitation	119:157	The application of chitosan/collagen/hyaluronic acid sponge cross-linked by dialdehyde starch addition as a matrix for calcium phosphate in situ precipitation.
28890371	10	19	theme	cross-linker	1232:1243	arg1	addition					1245:1252	The cross-linker addition	1228:1252	The cross-linker addition	1228:1252	The cross-linker addition and an inorganic compound precipitation improved the biocompatibility of the scaffolds.
28890371	7	20	theme	mechanical	952:961	arg1	parameters					963:972	The mechanical parameters	948:972	The mechanical parameters (i.e. compressive modulus and maximum compressive force)	948:1029	The mechanical parameters (i.e. compressive modulus and maximum compressive force), porosity, and density of the material were improved.
28890371	3	21	theme	SEM	471:473	arg1	images					475:480	SEM images	471:480	SEM images	471:480	Composites were characterized by different analyses, e.g. infrared spectroscopy, SEM images, porosity, density, and mechanical tests.
28890371	4	22	theme	X-ray	581:585	arg1	method					601:606	the energy dispersive X-ray spectroscopic method	559:606	the energy dispersive X-ray spectroscopic method	559:606	Moreover, an examination involving the energy dispersive X-ray spectroscopic method was carried out for the calcium and phosphorus ratio determination.
28890371	5	23	theme	obtained	776:783	arg1	scaffolds					785:793	the obtained scaffolds	772:793	the obtained scaffolds	772:793	In addition, the adhesion and proliferation of human osteosarcoma SaOS-2 cells were examined on the obtained scaffolds.
28890371	3	24	theme	infrared	448:455	arg1	spectroscopy					457:468	e.g. infrared spectroscopy	443:468	e.g. infrared spectroscopy	443:468	Composites were characterized by different analyses, e.g. infrared spectroscopy, SEM images, porosity, density, and mechanical tests.
28890371	7	25	theme	material	1061:1068	arg1	parameters					963:972	The mechanical parameters	948:972	The mechanical parameters (i.e. compressive modulus and maximum compressive force)	948:1029	The mechanical parameters (i.e. compressive modulus and maximum compressive force), porosity, and density of the material were improved.
28890371	7	25	theme	material	1061:1068	arg1	porosity					1032:1039	porosity	1032:1039	porosity	1032:1039	The mechanical parameters (i.e. compressive modulus and maximum compressive force), porosity, and density of the material were improved.
28890371	7	25	theme	material	1061:1068	arg1	density					1046:1052	density	1046:1052	density	1046:1052	The mechanical parameters (i.e. compressive modulus and maximum compressive force), porosity, and density of the material were improved.
28890371	4	26	theme	dispersive	570:579	arg1	method					601:606	the energy dispersive X-ray spectroscopic method	559:606	the energy dispersive X-ray spectroscopic method	559:606	Moreover, an examination involving the energy dispersive X-ray spectroscopic method was carried out for the calcium and phosphorus ratio determination.
28890371	0	27	theme	chitosan/collagen/hyaluronic	19:46	arg1	acid					48:51	chitosan/collagen/hyaluronic acid	19:51	chitosan/collagen/hyaluronic acid	19:51	The application of chitosan/collagen/hyaluronic acid sponge cross-linked by dialdehyde starch addition as a matrix for calcium phosphate in situ precipitation.
28890371	4	28	theme	energy	563:568	arg1	method					601:606	the energy dispersive X-ray spectroscopic method	559:606	the energy dispersive X-ray spectroscopic method	559:606	Moreover, an examination involving the energy dispersive X-ray spectroscopic method was carried out for the calcium and phosphorus ratio determination.
28890371	0	29	theme	in	137:138	arg1	precipitation					145:157	calcium phosphate in situ precipitation	119:157	calcium phosphate in situ precipitation	119:157	The application of chitosan/collagen/hyaluronic acid sponge cross-linked by dialdehyde starch addition as a matrix for calcium phosphate in situ precipitation.
28890371	7	30	theme	compressive	980:990	arg1	modulus					992:998	compressive modulus	980:998	compressive modulus	980:998	The mechanical parameters (i.e. compressive modulus and maximum compressive force), porosity, and density of the material were improved.
28890371	1	31	theme	hyaluronic	202:211	arg1	acid					213:216	hyaluronic acid	202:216	hyaluronic acid	202:216	Scaffolds based on chitosan, collagen and hyaluronic acid, cross-linked by dialdehyde starch were obtained through the freeze-drying method.
28890371	9	32	theme	SEM	1156:1158	arg1	images					1160:1165	SEM images	1156:1165	SEM images	1156:1165	SEM images showed the homogeneous structure, with interconnected pores.
28890371	4	33	theme	spectroscopic	587:599	arg1	method					601:606	the energy dispersive X-ray spectroscopic method	559:606	the energy dispersive X-ray spectroscopic method	559:606	Moreover, an examination involving the energy dispersive X-ray spectroscopic method was carried out for the calcium and phosphorus ratio determination.
28890371	0	34	theme	phosphate	127:135	arg1	precipitation					145:157	calcium phosphate in situ precipitation	119:157	calcium phosphate in situ precipitation	119:157	The application of chitosan/collagen/hyaluronic acid sponge cross-linked by dialdehyde starch addition as a matrix for calcium phosphate in situ precipitation.
28890371	6	35	theme	hyaluronic	885:894	arg1	acid					896:899	hyaluronic acid	885:899	hyaluronic acid	885:899	The results showed that the properties of the scaffolds based on chitosan, collagen, and hyaluronic acid can be modified by dialdehyde starch addition.
28890371	10	36	theme	scaffolds	1331:1339	arg1	biocompatibility					1307:1322	the biocompatibility	1303:1322	the biocompatibility of the scaffolds	1303:1339	The cross-linker addition and an inorganic compound precipitation improved the biocompatibility of the scaffolds.
28890371	4	37	theme	phosphorus	644:653	arg1	determination					661:673	the calcium and phosphorus ratio determination	628:673	the calcium and phosphorus ratio determination	628:673	Moreover, an examination involving the energy dispersive X-ray spectroscopic method was carried out for the calcium and phosphorus ratio determination.
28890371	3	38	theme	mechanical	506:515	arg1	tests					517:521	mechanical tests	506:521	mechanical tests	506:521	Composites were characterized by different analyses, e.g. infrared spectroscopy, SEM images, porosity, density, and mechanical tests.
28890371	5	39	theme	SaOS-2	742:747	arg1	cells					749:753	human osteosarcoma SaOS-2 cells	723:753	human osteosarcoma SaOS-2 cells	723:753	In addition, the adhesion and proliferation of human osteosarcoma SaOS-2 cells were examined on the obtained scaffolds.
28890371	8	40	from	ratio	1146:1150	arg1	scaffolds					1124:1132	the scaffolds	1120:1132	the scaffolds at the Ca/P ratio ∼2	1120:1153	Calcium phosphate was deposited in the scaffolds at the Ca/P ratio ∼2.
28890371	8	41	theme	Calcium	1085:1091	arg1	phosphate					1093:1101	Calcium phosphate	1085:1101	Calcium phosphate	1085:1101	Calcium phosphate was deposited in the scaffolds at the Ca/P ratio ∼2.
28890371	0	42	theme	acid	48:51	arg1	application					4:14	The application	0:14	The application of chitosan/collagen/hyaluronic acid	0:51	The application of chitosan/collagen/hyaluronic acid sponge cross-linked by dialdehyde starch addition as a matrix for calcium phosphate in situ precipitation.
28890371	3	43	theme	e.g.	443:446	arg1	spectroscopy					457:468	e.g. infrared spectroscopy	443:468	e.g. infrared spectroscopy	443:468	Composites were characterized by different analyses, e.g. infrared spectroscopy, SEM images, porosity, density, and mechanical tests.
28890371	11	44	theme	obtained	1346:1353	arg1	materials					1355:1363	The obtained materials	1342:1363	The obtained materials	1342:1363	The obtained materials can provide the support required in tissue engineering and regenerative medicine.
28890371	3	45	theme	different	423:431	arg1	analyses					433:440	different analyses	423:440	different analyses	423:440	Composites were characterized by different analyses, e.g. infrared spectroscopy, SEM images, porosity, density, and mechanical tests.
28890371	11	46	theme	regenerative	1424:1435	arg1	medicine					1437:1444	regenerative medicine	1424:1444	regenerative medicine	1424:1444	The obtained materials can provide the support required in tissue engineering and regenerative medicine.
28890371	0	47	dep	sponge	53:58	arg1	cross-linked					60:71	cross-linked	60:71	sponge cross-linked by dialdehyde starch addition as a matrix for calcium phosphate in situ precipitation	53:157	The application of chitosan/collagen/hyaluronic acid sponge cross-linked by dialdehyde starch addition as a matrix for calcium phosphate in situ precipitation.
28890371	2	48	theme	phosphate	357:365	arg1	precipitation					375:387	calcium phosphate in situ precipitation	349:387	calcium phosphate in situ precipitation	349:387	The porous structures were used as matrixes for calcium phosphate in situ precipitation.
28890371	5	49	theme	cells	749:753	arg1	proliferation					706:718	proliferation	706:718	proliferation	706:718	In addition, the adhesion and proliferation of human osteosarcoma SaOS-2 cells were examined on the obtained scaffolds.
28890371	5	49	theme	cells	749:753	arg1	adhesion					693:700	adhesion	693:700	adhesion	693:700	In addition, the adhesion and proliferation of human osteosarcoma SaOS-2 cells were examined on the obtained scaffolds.
28890371	5	50	theme	human	723:727	arg1	cells					749:753	human osteosarcoma SaOS-2 cells	723:753	human osteosarcoma SaOS-2 cells	723:753	In addition, the adhesion and proliferation of human osteosarcoma SaOS-2 cells were examined on the obtained scaffolds.
28890371	2	51	theme	calcium	349:355	arg1	precipitation					375:387	calcium phosphate in situ precipitation	349:387	calcium phosphate in situ precipitation	349:387	The porous structures were used as matrixes for calcium phosphate in situ precipitation.
28890371	4	52	dep	calcium	632:638	arg1	the					628:630	the	628:630	the	628:630	Moreover, an examination involving the energy dispersive X-ray spectroscopic method was carried out for the calcium and phosphorus ratio determination.
28890371	6	53	mod	modified	908:915	arg3	addition					938:945	dialdehyde starch addition	920:945	dialdehyde starch addition	920:945	The results showed that the properties of the scaffolds based on chitosan, collagen, and hyaluronic acid can be modified by dialdehyde starch addition.
28890371	6	53	mod	modified	908:915	arg1	properties					824:833	the properties	820:833	the properties of the scaffolds based on chitosan, collagen, and hyaluronic acid	820:899	The results showed that the properties of the scaffolds based on chitosan, collagen, and hyaluronic acid can be modified by dialdehyde starch addition.
28890371	7	54	theme	compressive	1012:1022	arg1	force					1024:1028	maximum compressive force	1004:1028	maximum compressive force	1004:1028	The mechanical parameters (i.e. compressive modulus and maximum compressive force), porosity, and density of the material were improved.
28890371	2	55	used	used	328:331	arg2	matrixes					336:343	matrixes	336:343	matrixes for calcium phosphate in situ precipitation	336:387	The porous structures were used as matrixes for calcium phosphate in situ precipitation.
28890371	2	55	used	used	328:331	arg2	structures					312:321	The porous structures	301:321	The porous structures	301:321	The porous structures were used as matrixes for calcium phosphate in situ precipitation.
28890371	1	56	theme	dialdehyde	235:244	arg1	starch					246:251	dialdehyde starch	235:251	dialdehyde starch	235:251	Scaffolds based on chitosan, collagen and hyaluronic acid, cross-linked by dialdehyde starch were obtained through the freeze-drying method.
28890371	0	57	theme	starch	87:92	arg1	addition					94:101	dialdehyde starch addition	76:101	dialdehyde starch addition	76:101	The application of chitosan/collagen/hyaluronic acid sponge cross-linked by dialdehyde starch addition as a matrix for calcium phosphate in situ precipitation.
28890371	4	58	theme	ratio	655:659	arg1	determination					661:673	the calcium and phosphorus ratio determination	628:673	the calcium and phosphorus ratio determination	628:673	Moreover, an examination involving the energy dispersive X-ray spectroscopic method was carried out for the calcium and phosphorus ratio determination.
28890371	7	59	dep	modulus	992:998	arg1	i.e.					975:978	i.e.	975:978	i.e.	975:978	The mechanical parameters (i.e. compressive modulus and maximum compressive force), porosity, and density of the material were improved.
28890371	0	60	theme	dialdehyde	76:85	arg1	addition					94:101	dialdehyde starch addition	76:101	dialdehyde starch addition	76:101	The application of chitosan/collagen/hyaluronic acid sponge cross-linked by dialdehyde starch addition as a matrix for calcium phosphate in situ precipitation.
28890371	4	61	theme	calcium	632:638	arg1	determination					661:673	the calcium and phosphorus ratio determination	628:673	the calcium and phosphorus ratio determination	628:673	Moreover, an examination involving the energy dispersive X-ray spectroscopic method was carried out for the calcium and phosphorus ratio determination.
24417502	3	0	theme	chitosan	358:365	arg1	matrix					367:372	the chitosan matrix	354:372	the chitosan matrix	354:372	Carbon nanofibers were homogeneously dispersed throughout the chitosan matrix, and the composite scaffold was highly porous with fully interconnected pores.
24417502	9	1	theme	carbon	1199:1204	arg1	nanofibers					1206:1215	carbon nanofibers	1199:1215	carbon nanofibers	1199:1215	This study demonstrates that the incorporation of carbon nanofibers into porous chitosan scaffolds improved the properties of cardiac tissue constructs, presumably through enhanced transmission of electrical signals between the cells.
24417502	7	2	from	cells	966:970	arg1	scaffolds					984:992	chitosan scaffolds	975:992	chitosan scaffolds	975:992	The metabolic activity of cells in chitosan/carbon constructs was significantly higher as compared to cells in chitosan scaffolds.
24417502	8	3	theme	electrical	1128:1137	arg1	coupling					1139:1146	electrical coupling	1128:1146	electrical coupling	1128:1146	The incorporation of carbon nanofibers also led to increased expression of cardiac-specific genes involved in muscle contraction and electrical coupling.
24417502	9	4	theme	electrical	1346:1355	arg1	signals					1357:1363	electrical signals	1346:1363	electrical signals	1346:1363	This study demonstrates that the incorporation of carbon nanofibers into porous chitosan scaffolds improved the properties of cardiac tissue constructs, presumably through enhanced transmission of electrical signals between the cells.
24417502	6	5	theme	construct	823:831	arg1	volume					833:838	the construct volume	819:838	the construct volume	819:838	After 14 days of culture, the scaffold pores throughout the construct volume were filled with cells.
24417502	9	6	theme	nanofibers	1206:1215	arg1	incorporation					1182:1194	the incorporation	1178:1194	the incorporation of carbon nanofibers into porous chitosan scaffolds	1178:1246	This study demonstrates that the incorporation of carbon nanofibers into porous chitosan scaffolds improved the properties of cardiac tissue constructs, presumably through enhanced transmission of electrical signals between the cells.
24417502	9	7	theme	constructs	1290:1299	arg1	properties					1261:1270	the properties	1257:1270	the properties of cardiac tissue constructs	1257:1299	This study demonstrates that the incorporation of carbon nanofibers into porous chitosan scaffolds improved the properties of cardiac tissue constructs, presumably through enhanced transmission of electrical signals between the cells.
24417502	9	8	theme	signals	1357:1363	arg1	transmission					1330:1341	enhanced transmission	1321:1341	enhanced transmission of electrical signals between the cells	1321:1381	This study demonstrates that the incorporation of carbon nanofibers into porous chitosan scaffolds improved the properties of cardiac tissue constructs, presumably through enhanced transmission of electrical signals between the cells.
24417502	5	9	theme	neonatal	674:681	arg1	cells					693:697	neonatal rat heart cells	674:697	neonatal rat heart cells	674:697	The scaffolds were seeded with neonatal rat heart cells and cultured for up to 14 days, without electrical stimulation.
24417502	6	10	theme	scaffold	793:800	arg1	pores					802:806	the scaffold pores	789:806	the scaffold pores throughout the construct volume	789:838	After 14 days of culture, the scaffold pores throughout the construct volume were filled with cells.
24417502	7	11	theme	metabolic	868:876	arg1	activity					878:885	The metabolic activity	864:885	The metabolic activity of cells in chitosan/carbon constructs	864:924	The metabolic activity of cells in chitosan/carbon constructs was significantly higher as compared to cells in chitosan scaffolds.
24417502	7	11	theme	metabolic	868:876	arg1	higher					944:949	higher	944:949	higher	944:949	The metabolic activity of cells in chitosan/carbon constructs was significantly higher as compared to cells in chitosan scaffolds.
24417502	4	12	theme	KPa	516:518	arg1	properties					591:600	excellent electrical properties	570:600	excellent electrical properties	570:600	Chitosan/carbon scaffolds had an elastic modulus of 28.1 ± 3.3 KPa, similar to that measured for rat myocardium, and excellent electrical properties, with a conductivity of 0.25 ± 0.09 S/m.
24417502	4	12	theme	KPa	516:518	arg1	modulus					494:500	an elastic modulus	483:500	an elastic modulus of 28.1 ± 3.3 KPa	483:518	Chitosan/carbon scaffolds had an elastic modulus of 28.1 ± 3.3 KPa, similar to that measured for rat myocardium, and excellent electrical properties, with a conductivity of 0.25 ± 0.09 S/m.
24417502	5	13	theme	rat	683:685	arg1	cells					693:697	neonatal rat heart cells	674:697	neonatal rat heart cells	674:697	The scaffolds were seeded with neonatal rat heart cells and cultured for up to 14 days, without electrical stimulation.
24417502	4	14	theme	Chitosan/carbon	453:467	arg1	scaffolds					469:477	Chitosan/carbon scaffolds	453:477	Chitosan/carbon scaffolds	453:477	Chitosan/carbon scaffolds had an elastic modulus of 28.1 ± 3.3 KPa, similar to that measured for rat myocardium, and excellent electrical properties, with a conductivity of 0.25 ± 0.09 S/m.
24417502	4	15	theme	3.3	512:514	arg1	±					510:510	±	510:510	±	510:510	Chitosan/carbon scaffolds had an elastic modulus of 28.1 ± 3.3 KPa, similar to that measured for rat myocardium, and excellent electrical properties, with a conductivity of 0.25 ± 0.09 S/m.
24417502	9	16	theme	chitosan	1229:1236	arg1	scaffolds					1238:1246	porous chitosan scaffolds	1222:1246	porous chitosan scaffolds	1222:1246	This study demonstrates that the incorporation of carbon nanofibers into porous chitosan scaffolds improved the properties of cardiac tissue constructs, presumably through enhanced transmission of electrical signals between the cells.
24417502	7	17	from	activity	878:885	arg1	constructs					915:924	chitosan/carbon constructs	899:924	chitosan/carbon constructs	899:924	The metabolic activity of cells in chitosan/carbon constructs was significantly higher as compared to cells in chitosan scaffolds.
24417502	1	18	theme	conductive	163:172	arg1	composite					189:197	a highly conductive chitosan-based composite	154:197	a highly conductive chitosan-based composite	154:197	In this work, carbon nanofibers were used as doping material to develop a highly conductive chitosan-based composite.
24417502	3	19	theme	composite	383:391	arg1	scaffold					393:400	the composite scaffold	379:400	the composite scaffold	379:400	Carbon nanofibers were homogeneously dispersed throughout the chitosan matrix, and the composite scaffold was highly porous with fully interconnected pores.
24417502	3	19	theme	composite	383:391	arg1	porous					413:418	porous	413:418	porous	413:418	Carbon nanofibers were homogeneously dispersed throughout the chitosan matrix, and the composite scaffold was highly porous with fully interconnected pores.
24417502	4	20	theme	similar	521:527	arg1	modulus					494:500	an elastic modulus	483:500	an elastic modulus of 28.1 ± 3.3 KPa	483:518	Chitosan/carbon scaffolds had an elastic modulus of 28.1 ± 3.3 KPa, similar to that measured for rat myocardium, and excellent electrical properties, with a conductivity of 0.25 ± 0.09 S/m.
24417502	1	21	theme	chitosan-based	174:187	arg1	composite					189:197	a highly conductive chitosan-based composite	154:197	a highly conductive chitosan-based composite	154:197	In this work, carbon nanofibers were used as doping material to develop a highly conductive chitosan-based composite.
24417502	0	22	theme	conductive	13:22	arg1	chitosan/carbon					24:38	Electrically conductive chitosan/carbon	0:38	Electrically conductive chitosan/carbon	0:38	Electrically conductive chitosan/carbon scaffolds for cardiac tissue engineering.
24417502	8	23	theme	genes	1087:1091	arg1	expression					1056:1065	increased expression	1046:1065	increased expression of cardiac-specific genes involved in muscle contraction and electrical coupling	1046:1146	The incorporation of carbon nanofibers also led to increased expression of cardiac-specific genes involved in muscle contraction and electrical coupling.
24417502	6	24	theme	culture	780:786	arg1	days					772:775	14 days	769:775	14 days of culture	769:786	After 14 days of culture, the scaffold pores throughout the construct volume were filled with cells.
24417502	0	25	theme	Electrically	0:11	arg1	chitosan/carbon					24:38	Electrically conductive chitosan/carbon	0:38	Electrically conductive chitosan/carbon	0:38	Electrically conductive chitosan/carbon scaffolds for cardiac tissue engineering.
24417502	4	26	theme	electrical	580:589	arg1	properties					591:600	excellent electrical properties	570:600	excellent electrical properties	570:600	Chitosan/carbon scaffolds had an elastic modulus of 28.1 ± 3.3 KPa, similar to that measured for rat myocardium, and excellent electrical properties, with a conductivity of 0.25 ± 0.09 S/m.
24417502	8	27	theme	cardiac-specific	1070:1085	arg1	genes					1087:1091	cardiac-specific genes	1070:1091	cardiac-specific genes involved in muscle contraction and electrical coupling	1070:1146	The incorporation of carbon nanofibers also led to increased expression of cardiac-specific genes involved in muscle contraction and electrical coupling.
24417502	8	28	theme	nanofibers	1023:1032	arg1	incorporation					999:1011	The incorporation	995:1011	The incorporation of carbon nanofibers	995:1032	The incorporation of carbon nanofibers also led to increased expression of cardiac-specific genes involved in muscle contraction and electrical coupling.
24417502	1	29	theme	carbon	96:101	arg1	material					134:141	doping material	127:141	doping material	127:141	In this work, carbon nanofibers were used as doping material to develop a highly conductive chitosan-based composite.
24417502	1	29	theme	carbon	96:101	arg1	nanofibers					103:112	carbon nanofibers	96:112	carbon nanofibers	96:112	In this work, carbon nanofibers were used as doping material to develop a highly conductive chitosan-based composite.
24417502	4	30	contain	had	479:481	arg1	scaffolds					469:477	Chitosan/carbon scaffolds	453:477	Chitosan/carbon scaffolds	453:477	Chitosan/carbon scaffolds had an elastic modulus of 28.1 ± 3.3 KPa, similar to that measured for rat myocardium, and excellent electrical properties, with a conductivity of 0.25 ± 0.09 S/m.
24417502	4	30	contain	had	479:481	arg2	properties					591:600	excellent electrical properties	570:600	excellent electrical properties	570:600	Chitosan/carbon scaffolds had an elastic modulus of 28.1 ± 3.3 KPa, similar to that measured for rat myocardium, and excellent electrical properties, with a conductivity of 0.25 ± 0.09 S/m.
24417502	4	30	contain	had	479:481	arg2	modulus					494:500	an elastic modulus	483:500	an elastic modulus of 28.1 ± 3.3 KPa	483:518	Chitosan/carbon scaffolds had an elastic modulus of 28.1 ± 3.3 KPa, similar to that measured for rat myocardium, and excellent electrical properties, with a conductivity of 0.25 ± 0.09 S/m.
24417502	9	31	theme	porous	1222:1227	arg1	scaffolds					1238:1246	porous chitosan scaffolds	1222:1246	porous chitosan scaffolds	1222:1246	This study demonstrates that the incorporation of carbon nanofibers into porous chitosan scaffolds improved the properties of cardiac tissue constructs, presumably through enhanced transmission of electrical signals between the cells.
24417502	4	32	theme	excellent	570:578	arg1	properties					591:600	excellent electrical properties	570:600	excellent electrical properties	570:600	Chitosan/carbon scaffolds had an elastic modulus of 28.1 ± 3.3 KPa, similar to that measured for rat myocardium, and excellent electrical properties, with a conductivity of 0.25 ± 0.09 S/m.
24417502	8	33	theme	carbon	1016:1021	arg1	nanofibers					1023:1032	carbon nanofibers	1016:1032	carbon nanofibers	1016:1032	The incorporation of carbon nanofibers also led to increased expression of cardiac-specific genes involved in muscle contraction and electrical coupling.
24417502	3	34	with	porous	413:418	arg1	pores					446:450	fully interconnected pores	425:450	fully interconnected pores	425:450	Carbon nanofibers were homogeneously dispersed throughout the chitosan matrix, and the composite scaffold was highly porous with fully interconnected pores.
24417502	1	35	used	used	119:122	arg2	nanofibers					103:112	carbon nanofibers	96:112	carbon nanofibers	96:112	In this work, carbon nanofibers were used as doping material to develop a highly conductive chitosan-based composite.
24417502	1	35	used	used	119:122	arg2	material					134:141	doping material	127:141	doping material	127:141	In this work, carbon nanofibers were used as doping material to develop a highly conductive chitosan-based composite.
24417502	0	36	theme	cardiac	54:60	arg1	engineering					69:79	cardiac tissue engineering	54:79	cardiac tissue engineering	54:79	Electrically conductive chitosan/carbon scaffolds for cardiac tissue engineering.
24417502	9	37	theme	cardiac	1275:1281	arg1	constructs					1290:1299	cardiac tissue constructs	1275:1299	cardiac tissue constructs	1275:1299	This study demonstrates that the incorporation of carbon nanofibers into porous chitosan scaffolds improved the properties of cardiac tissue constructs, presumably through enhanced transmission of electrical signals between the cells.
24417502	3	38	theme	Carbon	296:301	arg1	nanofibers					303:312	Carbon nanofibers	296:312	Carbon nanofibers	296:312	Carbon nanofibers were homogeneously dispersed throughout the chitosan matrix, and the composite scaffold was highly porous with fully interconnected pores.
24417502	9	39	theme	tissue	1283:1288	arg1	constructs					1290:1299	cardiac tissue constructs	1275:1299	cardiac tissue constructs	1275:1299	This study demonstrates that the incorporation of carbon nanofibers into porous chitosan scaffolds improved the properties of cardiac tissue constructs, presumably through enhanced transmission of electrical signals between the cells.
24417502	2	40	theme	chitosan/carbon	237:251	arg1	composites					253:262	chitosan/carbon composites	237:262	chitosan/carbon composites	237:262	Scaffolds based on chitosan only and chitosan/carbon composites were prepared by precipitation.
24417502	5	41	theme	electrical	739:748	arg1	stimulation					750:760	electrical stimulation	739:760	electrical stimulation	739:760	The scaffolds were seeded with neonatal rat heart cells and cultured for up to 14 days, without electrical stimulation.
24417502	3	42	theme	interconnected	431:444	arg1	pores					446:450	fully interconnected pores	425:450	fully interconnected pores	425:450	Carbon nanofibers were homogeneously dispersed throughout the chitosan matrix, and the composite scaffold was highly porous with fully interconnected pores.
24417502	4	43	theme	rat	550:552	arg1	myocardium					554:563	rat myocardium	550:563	rat myocardium	550:563	Chitosan/carbon scaffolds had an elastic modulus of 28.1 ± 3.3 KPa, similar to that measured for rat myocardium, and excellent electrical properties, with a conductivity of 0.25 ± 0.09 S/m.
24417502	5	44	theme	heart	687:691	arg1	cells					693:697	neonatal rat heart cells	674:697	neonatal rat heart cells	674:697	The scaffolds were seeded with neonatal rat heart cells and cultured for up to 14 days, without electrical stimulation.
24417502	4	45	theme	28.1	505:508	arg1	±					510:510	±	510:510	±	510:510	Chitosan/carbon scaffolds had an elastic modulus of 28.1 ± 3.3 KPa, similar to that measured for rat myocardium, and excellent electrical properties, with a conductivity of 0.25 ± 0.09 S/m.
24417502	0	46	theme	tissue	62:67	arg1	engineering					69:79	cardiac tissue engineering	54:79	cardiac tissue engineering	54:79	Electrically conductive chitosan/carbon scaffolds for cardiac tissue engineering.
24417502	8	47	theme	increased	1046:1054	arg1	expression					1056:1065	increased expression	1046:1065	increased expression of cardiac-specific genes involved in muscle contraction and electrical coupling	1046:1146	The incorporation of carbon nanofibers also led to increased expression of cardiac-specific genes involved in muscle contraction and electrical coupling.
24417502	7	48	theme	chitosan/carbon	899:913	arg1	constructs					915:924	chitosan/carbon constructs	899:924	chitosan/carbon constructs	899:924	The metabolic activity of cells in chitosan/carbon constructs was significantly higher as compared to cells in chitosan scaffolds.
24417502	1	49	theme	doping	127:132	arg1	material					134:141	doping material	127:141	doping material	127:141	In this work, carbon nanofibers were used as doping material to develop a highly conductive chitosan-based composite.
24417502	1	49	theme	doping	127:132	arg1	nanofibers					103:112	carbon nanofibers	96:112	carbon nanofibers	96:112	In this work, carbon nanofibers were used as doping material to develop a highly conductive chitosan-based composite.
24417502	4	50	theme	elastic	486:492	arg1	modulus					494:500	an elastic modulus	483:500	an elastic modulus of 28.1 ± 3.3 KPa	483:518	Chitosan/carbon scaffolds had an elastic modulus of 28.1 ± 3.3 KPa, similar to that measured for rat myocardium, and excellent electrical properties, with a conductivity of 0.25 ± 0.09 S/m.
24417502	7	51	theme	cells	890:894	arg1	activity					878:885	The metabolic activity	864:885	The metabolic activity of cells in chitosan/carbon constructs	864:924	The metabolic activity of cells in chitosan/carbon constructs was significantly higher as compared to cells in chitosan scaffolds.
24417502	7	51	theme	cells	890:894	arg1	higher					944:949	higher	944:949	higher	944:949	The metabolic activity of cells in chitosan/carbon constructs was significantly higher as compared to cells in chitosan scaffolds.
24417502	9	52	theme	enhanced	1321:1328	arg1	transmission					1330:1341	enhanced transmission	1321:1341	enhanced transmission of electrical signals between the cells	1321:1381	This study demonstrates that the incorporation of carbon nanofibers into porous chitosan scaffolds improved the properties of cardiac tissue constructs, presumably through enhanced transmission of electrical signals between the cells.
24417502	4	53	theme	S/m	638:640	arg1	conductivity					610:621	a conductivity	608:621	a conductivity of 0.25 ± 0.09 S/m	608:640	Chitosan/carbon scaffolds had an elastic modulus of 28.1 ± 3.3 KPa, similar to that measured for rat myocardium, and excellent electrical properties, with a conductivity of 0.25 ± 0.09 S/m.
24417502	5	54	dep	14	722:723	arg1	to					719:720	to	719:720	to	719:720	The scaffolds were seeded with neonatal rat heart cells and cultured for up to 14 days, without electrical stimulation.
24417502	7	55	theme	chitosan	975:982	arg1	scaffolds					984:992	chitosan scaffolds	975:992	chitosan scaffolds	975:992	The metabolic activity of cells in chitosan/carbon constructs was significantly higher as compared to cells in chitosan scaffolds.
24417502	8	56	theme	muscle	1105:1110	arg1	contraction					1112:1122	muscle contraction	1105:1122	muscle contraction	1105:1122	The incorporation of carbon nanofibers also led to increased expression of cardiac-specific genes involved in muscle contraction and electrical coupling.
28259765	0	0	theme	Coacervate	79:88	arg1	Compositions					90:101	Cellulosic Coacervate Compositions	68:101	Cellulosic Coacervate Compositions	68:101	Evaluating the Transport Kinetics of a Model Compound Released From Cellulosic Coacervate Compositions Into Artificial Sebum.
28259765	8	1	theme	in vitro	1346:1353	arg1	method					1371:1376	The in vitro release testing method	1342:1376	The in vitro release testing method	1342:1376	The in vitro release testing method proved capable of discriminating between clinically relevant differences in transport kinetics from different coacervate formulations using a practical sample size.
28259765	8	2	theme	release	1355:1361	arg1	method					1371:1376	The in vitro release testing method	1342:1376	The in vitro release testing method	1342:1376	The in vitro release testing method proved capable of discriminating between clinically relevant differences in transport kinetics from different coacervate formulations using a practical sample size.
28259765	0	3	theme	Cellulosic	68:77	arg1	Compositions					90:101	Cellulosic Coacervate Compositions	68:101	Cellulosic Coacervate Compositions	68:101	Evaluating the Transport Kinetics of a Model Compound Released From Cellulosic Coacervate Compositions Into Artificial Sebum.
28259765	8	4	theme	coacervate	1488:1497	arg1	formulations					1499:1510	different coacervate formulations	1478:1510	different coacervate formulations using a practical sample size	1478:1540	The in vitro release testing method proved capable of discriminating between clinically relevant differences in transport kinetics from different coacervate formulations using a practical sample size.
28259765	6	5	theme	Steady-state	1082:1093	arg1	flux					1095:1098	Steady-state flux	1082:1098	Steady-state flux of the model compound from each coacervate composition	1082:1153	Steady-state flux of the model compound from each coacervate composition was found to vary with water content of the composition.
28259765	1	6	theme	anionic	171:177	arg1	surfactants					179:189	anionic surfactants	171:189	anionic surfactants	171:189	Complex coacervates of cationic polymers and anionic surfactants, which are produced spontaneously during the use of rinse-off formulations, represent an important delivery vehicle for topical agents to the skin surface and appendages.
28259765	8	7	from	differences	1439:1449	arg1	kinetics					1464:1471	transport kinetics	1454:1471	transport kinetics from different coacervate formulations using a practical sample size	1454:1540	The in vitro release testing method proved capable of discriminating between clinically relevant differences in transport kinetics from different coacervate formulations using a practical sample size.
28259765	8	7	from	differences	1439:1449	arg1	formulations					1499:1510	different coacervate formulations	1478:1510	different coacervate formulations using a practical sample size	1478:1540	The in vitro release testing method proved capable of discriminating between clinically relevant differences in transport kinetics from different coacervate formulations using a practical sample size.
28259765	8	8	theme	testing	1363:1369	arg1	method					1371:1376	The in vitro release testing method	1342:1376	The in vitro release testing method	1342:1376	The in vitro release testing method proved capable of discriminating between clinically relevant differences in transport kinetics from different coacervate formulations using a practical sample size.
28259765	7	9	theme	fraction	1273:1280	arg1	 ×					1258:1259	[(cat-HECDS:NaDS) ×	1241:1259	[(cat-HECDS:NaDS) × (1 - weight fraction water)	1241:1287	When flux was plotted versus [(cat-HECDS:NaDS) × (1 - weight fraction water)]-1, a strong linear correlation (R2 = 0.89) emerged.
28259765	7	9	theme	fraction	1273:1280	arg1	water					1282:1286	1 - weight fraction water	1262:1286	1 - weight fraction water	1262:1286	When flux was plotted versus [(cat-HECDS:NaDS) × (1 - weight fraction water)]-1, a strong linear correlation (R2 = 0.89) emerged.
28259765	8	10	from	formulations	1499:1510	arg1	differences					1439:1449	clinically relevant differences	1419:1449	clinically relevant differences in transport kinetics from different coacervate formulations using a practical sample size	1419:1540	The in vitro release testing method proved capable of discriminating between clinically relevant differences in transport kinetics from different coacervate formulations using a practical sample size.
28259765	8	10	from	formulations	1499:1510	arg1	kinetics					1464:1471	transport kinetics	1454:1471	transport kinetics from different coacervate formulations using a practical sample size	1454:1540	The in vitro release testing method proved capable of discriminating between clinically relevant differences in transport kinetics from different coacervate formulations using a practical sample size.
28259765	4	11	theme	sodium	804:809	arg1	NaDS					828:831	NaDS	828:831	NaDS	828:831	Coacervate compositions were prepared with cationic-hydroxyethyl cellulose dodecyl sulfate (cat-HECDS), sodium dodecyl sulfate (NaDS), and water.
28259765	4	11	theme	sodium	804:809	arg1	sulfate					819:825	sodium dodecyl sulfate	804:825	sodium dodecyl sulfate (NaDS)	804:832	Coacervate compositions were prepared with cationic-hydroxyethyl cellulose dodecyl sulfate (cat-HECDS), sodium dodecyl sulfate (NaDS), and water.
28259765	1	12	theme	important	280:288	arg1	vehicle					299:305	an important delivery vehicle	277:305	an important delivery vehicle for topical agents to the skin surface and appendages	277:359	Complex coacervates of cationic polymers and anionic surfactants, which are produced spontaneously during the use of rinse-off formulations, represent an important delivery vehicle for topical agents to the skin surface and appendages.
28259765	6	13	theme	compound	1113:1120	arg1	flux					1095:1098	Steady-state flux	1082:1098	Steady-state flux of the model compound from each coacervate composition	1082:1153	Steady-state flux of the model compound from each coacervate composition was found to vary with water content of the composition.
28259765	3	14	attach	released	639:646	arg2	kinetin					630:636	kinetin	630:636	kinetin	630:636	This method was subsequently used to evaluate the transport kinetics of a model compound, kinetin, released from semi-solid coacervate formulations into sebum.
28259765	3	14	attach	released	639:646	arg2	compound					620:627	a model compound	612:627	a model compound	612:627	This method was subsequently used to evaluate the transport kinetics of a model compound, kinetin, released from semi-solid coacervate formulations into sebum.
28259765	3	14	attach	released	639:646	arg1	formulations					675:686	semi-solid coacervate formulations	653:686	semi-solid coacervate formulations	653:686	This method was subsequently used to evaluate the transport kinetics of a model compound, kinetin, released from semi-solid coacervate formulations into sebum.
28259765	7	15	theme	strong	1295:1300	arg1	R2 =					1322:1325	R2 = 0.89	1322:1330	R2 = 0.89	1322:1330	When flux was plotted versus [(cat-HECDS:NaDS) × (1 - weight fraction water)]-1, a strong linear correlation (R2 = 0.89) emerged.
28259765	7	15	theme	strong	1295:1300	arg1	correlation					1309:1319	a strong linear correlation	1293:1319	a strong linear correlation (R2 = 0.89)	1293:1331	When flux was plotted versus [(cat-HECDS:NaDS) × (1 - weight fraction water)]-1, a strong linear correlation (R2 = 0.89) emerged.
28259765	3	16	used	used	569:572	arg2	method					545:550	This method	540:550	This method	540:550	This method was subsequently used to evaluate the transport kinetics of a model compound, kinetin, released from semi-solid coacervate formulations into sebum.
28259765	1	17	theme	surfactants	179:189	arg1	coacervates					134:144	Complex coacervates	126:144	Complex coacervates	126:144	Complex coacervates of cationic polymers and anionic surfactants, which are produced spontaneously during the use of rinse-off formulations, represent an important delivery vehicle for topical agents to the skin surface and appendages.
28259765	0	18	theme	Artificial	108:117	arg1	Sebum					119:123	Artificial Sebum	108:123	Artificial Sebum	108:123	Evaluating the Transport Kinetics of a Model Compound Released From Cellulosic Coacervate Compositions Into Artificial Sebum.
28259765	8	19	theme	different	1478:1486	arg1	formulations					1499:1510	different coacervate formulations	1478:1510	different coacervate formulations using a practical sample size	1478:1540	The in vitro release testing method proved capable of discriminating between clinically relevant differences in transport kinetics from different coacervate formulations using a practical sample size.
28259765	4	20	theme	dodecyl	811:817	arg1	NaDS					828:831	NaDS	828:831	NaDS	828:831	Coacervate compositions were prepared with cationic-hydroxyethyl cellulose dodecyl sulfate (cat-HECDS), sodium dodecyl sulfate (NaDS), and water.
28259765	4	20	theme	dodecyl	811:817	arg1	sulfate					819:825	sodium dodecyl sulfate	804:825	sodium dodecyl sulfate (NaDS)	804:832	Coacervate compositions were prepared with cationic-hydroxyethyl cellulose dodecyl sulfate (cat-HECDS), sodium dodecyl sulfate (NaDS), and water.
28259765	2	21	theme	sebum-loaded	391:402	arg1	method					424:429	an artificial sebum-loaded cell culture insert method	377:429	an artificial sebum-loaded cell culture insert method for determining the sebum diffusion properties of topical agents	377:494	In this study, an artificial sebum-loaded cell culture insert method for determining the sebum diffusion properties of topical agents was optimized for in vitro release testing.
28259765	2	22	theme	diffusion	457:465	arg1	properties					467:476	the sebum diffusion properties	447:476	the sebum diffusion properties of topical agents	447:494	In this study, an artificial sebum-loaded cell culture insert method for determining the sebum diffusion properties of topical agents was optimized for in vitro release testing.
28259765	5	23	theme	%	927:927	arg1	ratio					929:933	wt% ratio	925:933	wt% ratio of 2:1	925:940	Tested compositions ranged from 90 to 50 wt% water and had a cat-HECDS to NaDS wt% ratio of 2:1, 1:1, or 1:2, mimicking the in vivo hydration range and relative excess surfactant content expected from commercial rinse-off formulations.
28259765	8	24	theme	transport	1454:1462	arg1	kinetics					1464:1471	transport kinetics	1454:1471	transport kinetics from different coacervate formulations using a practical sample size	1454:1540	The in vitro release testing method proved capable of discriminating between clinically relevant differences in transport kinetics from different coacervate formulations using a practical sample size.
28259765	2	25	theme	artificial	380:389	arg1	method					424:429	an artificial sebum-loaded cell culture insert method	377:429	an artificial sebum-loaded cell culture insert method for determining the sebum diffusion properties of topical agents	377:494	In this study, an artificial sebum-loaded cell culture insert method for determining the sebum diffusion properties of topical agents was optimized for in vitro release testing.
28259765	2	26	theme	sebum	451:455	arg1	properties					467:476	the sebum diffusion properties	447:476	the sebum diffusion properties of topical agents	447:494	In this study, an artificial sebum-loaded cell culture insert method for determining the sebum diffusion properties of topical agents was optimized for in vitro release testing.
28259765	1	27	theme	delivery	290:297	arg1	vehicle					299:305	an important delivery vehicle	277:305	an important delivery vehicle for topical agents to the skin surface and appendages	277:359	Complex coacervates of cationic polymers and anionic surfactants, which are produced spontaneously during the use of rinse-off formulations, represent an important delivery vehicle for topical agents to the skin surface and appendages.
28259765	5	28	dep	range	988:992	arg1	the					966:968	the	966:968	the	966:968	Tested compositions ranged from 90 to 50 wt% water and had a cat-HECDS to NaDS wt% ratio of 2:1, 1:1, or 1:2, mimicking the in vivo hydration range and relative excess surfactant content expected from commercial rinse-off formulations.
28259765	8	29	theme	practical	1520:1528	arg1	size					1537:1540	a practical sample size	1518:1540	a practical sample size	1518:1540	The in vitro release testing method proved capable of discriminating between clinically relevant differences in transport kinetics from different coacervate formulations using a practical sample size.
28259765	3	30	theme	transport	590:598	arg1	kinetics					600:607	the transport kinetics	586:607	the transport kinetics of a model compound, kinetin, released from semi-solid coacervate formulations into sebum	586:697	This method was subsequently used to evaluate the transport kinetics of a model compound, kinetin, released from semi-solid coacervate formulations into sebum.
28259765	5	31	theme	%	889:889	arg1	water					891:895	wt% water	887:895	90 to 50 wt% water	878:895	Tested compositions ranged from 90 to 50 wt% water and had a cat-HECDS to NaDS wt% ratio of 2:1, 1:1, or 1:2, mimicking the in vivo hydration range and relative excess surfactant content expected from commercial rinse-off formulations.
28259765	6	32	theme	water	1178:1182	arg1	content					1184:1190	water content	1178:1190	water content of the composition	1178:1209	Steady-state flux of the model compound from each coacervate composition was found to vary with water content of the composition.
28259765	5	33	theme	wt	925:926	arg1	ratio					929:933	wt% ratio	925:933	wt% ratio of 2:1	925:940	Tested compositions ranged from 90 to 50 wt% water and had a cat-HECDS to NaDS wt% ratio of 2:1, 1:1, or 1:2, mimicking the in vivo hydration range and relative excess surfactant content expected from commercial rinse-off formulations.
28259765	7	34	theme	linear	1302:1307	arg1	R2 =					1322:1325	R2 = 0.89	1322:1330	R2 = 0.89	1322:1330	When flux was plotted versus [(cat-HECDS:NaDS) × (1 - weight fraction water)]-1, a strong linear correlation (R2 = 0.89) emerged.
28259765	7	34	theme	linear	1302:1307	arg1	correlation					1309:1319	a strong linear correlation	1293:1319	a strong linear correlation (R2 = 0.89)	1293:1331	When flux was plotted versus [(cat-HECDS:NaDS) × (1 - weight fraction water)]-1, a strong linear correlation (R2 = 0.89) emerged.
28259765	0	35	theme	Transport	15:23	arg1	Kinetics					25:32	the Transport Kinetics	11:32	the Transport Kinetics of a Model Compound Released From Cellulosic Coacervate Compositions Into Artificial Sebum	11:123	Evaluating the Transport Kinetics of a Model Compound Released From Cellulosic Coacervate Compositions Into Artificial Sebum.
28259765	7	36	dep	cat-HECDS	1243:1251	arg1	NaDS					1253:1256	NaDS	1253:1256	cat-HECDS:NaDS	1243:1256	When flux was plotted versus [(cat-HECDS:NaDS) × (1 - weight fraction water)]-1, a strong linear correlation (R2 = 0.89) emerged.
28259765	3	37	theme	semi-solid	653:662	arg1	formulations					675:686	semi-solid coacervate formulations	653:686	semi-solid coacervate formulations	653:686	This method was subsequently used to evaluate the transport kinetics of a model compound, kinetin, released from semi-solid coacervate formulations into sebum.
28259765	5	38	theme	2:1	938:940	arg1	1:1					943:945	1:1	943:945	1:1	943:945	Tested compositions ranged from 90 to 50 wt% water and had a cat-HECDS to NaDS wt% ratio of 2:1, 1:1, or 1:2, mimicking the in vivo hydration range and relative excess surfactant content expected from commercial rinse-off formulations.
28259765	5	38	theme	2:1	938:940	arg1	1:2					951:953	1:2	951:953	1:2	951:953	Tested compositions ranged from 90 to 50 wt% water and had a cat-HECDS to NaDS wt% ratio of 2:1, 1:1, or 1:2, mimicking the in vivo hydration range and relative excess surfactant content expected from commercial rinse-off formulations.
28259765	5	38	theme	2:1	938:940	arg1	ratio					929:933	wt% ratio	925:933	wt% ratio of 2:1	925:940	Tested compositions ranged from 90 to 50 wt% water and had a cat-HECDS to NaDS wt% ratio of 2:1, 1:1, or 1:2, mimicking the in vivo hydration range and relative excess surfactant content expected from commercial rinse-off formulations.
28259765	6	39	from	composition	1143:1153	arg1	flux					1095:1098	Steady-state flux	1082:1098	Steady-state flux of the model compound from each coacervate composition	1082:1153	Steady-state flux of the model compound from each coacervate composition was found to vary with water content of the composition.
28259765	6	40	theme	coacervate	1132:1141	arg1	composition					1143:1153	each coacervate composition	1127:1153	each coacervate composition	1127:1153	Steady-state flux of the model compound from each coacervate composition was found to vary with water content of the composition.
28259765	2	41	theme	agents	489:494	arg1	properties					467:476	the sebum diffusion properties	447:476	the sebum diffusion properties of topical agents	447:494	In this study, an artificial sebum-loaded cell culture insert method for determining the sebum diffusion properties of topical agents was optimized for in vitro release testing.
28259765	1	42	theme	topical	311:317	arg1	agents					319:324	topical agents	311:324	topical agents	311:324	Complex coacervates of cationic polymers and anionic surfactants, which are produced spontaneously during the use of rinse-off formulations, represent an important delivery vehicle for topical agents to the skin surface and appendages.
28259765	7	43	dep	 ×	1258:1259	arg1	cat-HECDS					1243:1251	cat-HECDS	1243:1251	cat-HECDS:NaDS	1243:1256	When flux was plotted versus [(cat-HECDS:NaDS) × (1 - weight fraction water)]-1, a strong linear correlation (R2 = 0.89) emerged.
28259765	3	44	theme	coacervate	664:673	arg1	formulations					675:686	semi-solid coacervate formulations	653:686	semi-solid coacervate formulations	653:686	This method was subsequently used to evaluate the transport kinetics of a model compound, kinetin, released from semi-solid coacervate formulations into sebum.
28259765	5	45	theme	in vivo	970:976	arg1	range					988:992	in vivo hydration range	970:992	in vivo hydration range	970:992	Tested compositions ranged from 90 to 50 wt% water and had a cat-HECDS to NaDS wt% ratio of 2:1, 1:1, or 1:2, mimicking the in vivo hydration range and relative excess surfactant content expected from commercial rinse-off formulations.
28259765	7	46	theme	weight	1266:1271	arg1	 ×					1258:1259	[(cat-HECDS:NaDS) ×	1241:1259	[(cat-HECDS:NaDS) × (1 - weight fraction water)	1241:1287	When flux was plotted versus [(cat-HECDS:NaDS) × (1 - weight fraction water)]-1, a strong linear correlation (R2 = 0.89) emerged.
28259765	7	46	theme	weight	1266:1271	arg1	water					1282:1286	1 - weight fraction water	1262:1286	1 - weight fraction water	1262:1286	When flux was plotted versus [(cat-HECDS:NaDS) × (1 - weight fraction water)]-1, a strong linear correlation (R2 = 0.89) emerged.
28259765	2	47	theme	insert	417:422	arg1	method					424:429	an artificial sebum-loaded cell culture insert method	377:429	an artificial sebum-loaded cell culture insert method for determining the sebum diffusion properties of topical agents	377:494	In this study, an artificial sebum-loaded cell culture insert method for determining the sebum diffusion properties of topical agents was optimized for in vitro release testing.
28259765	2	48	theme	cell	404:407	arg1	method					424:429	an artificial sebum-loaded cell culture insert method	377:429	an artificial sebum-loaded cell culture insert method for determining the sebum diffusion properties of topical agents	377:494	In this study, an artificial sebum-loaded cell culture insert method for determining the sebum diffusion properties of topical agents was optimized for in vitro release testing.
28259765	8	49	theme	sample	1530:1535	arg1	size					1537:1540	a practical sample size	1518:1540	a practical sample size	1518:1540	The in vitro release testing method proved capable of discriminating between clinically relevant differences in transport kinetics from different coacervate formulations using a practical sample size.
28259765	2	50	theme	topical	481:487	arg1	agents					489:494	topical agents	481:494	topical agents	481:494	In this study, an artificial sebum-loaded cell culture insert method for determining the sebum diffusion properties of topical agents was optimized for in vitro release testing.
28259765	5	51	theme	commercial	1047:1056	arg1	formulations					1068:1079	commercial rinse-off formulations	1047:1079	commercial rinse-off formulations	1047:1079	Tested compositions ranged from 90 to 50 wt% water and had a cat-HECDS to NaDS wt% ratio of 2:1, 1:1, or 1:2, mimicking the in vivo hydration range and relative excess surfactant content expected from commercial rinse-off formulations.
28259765	3	52	theme	model	614:618	arg1	kinetin					630:636	kinetin	630:636	kinetin	630:636	This method was subsequently used to evaluate the transport kinetics of a model compound, kinetin, released from semi-solid coacervate formulations into sebum.
28259765	3	52	theme	model	614:618	arg1	compound					620:627	a model compound	612:627	a model compound	612:627	This method was subsequently used to evaluate the transport kinetics of a model compound, kinetin, released from semi-solid coacervate formulations into sebum.
28259765	0	53	attach	Released	54:61	arg2	Compound					45:52	a Model Compound	37:52	a Model Compound Released From Cellulosic Coacervate Compositions Into Artificial Sebum	37:123	Evaluating the Transport Kinetics of a Model Compound Released From Cellulosic Coacervate Compositions Into Artificial Sebum.
28259765	0	53	attach	Released	54:61	arg1	Compositions					90:101	Cellulosic Coacervate Compositions	68:101	Cellulosic Coacervate Compositions	68:101	Evaluating the Transport Kinetics of a Model Compound Released From Cellulosic Coacervate Compositions Into Artificial Sebum.
28259765	2	54	theme	release	523:529	arg1	testing					531:537	in vitro release testing	514:537	in vitro release testing	514:537	In this study, an artificial sebum-loaded cell culture insert method for determining the sebum diffusion properties of topical agents was optimized for in vitro release testing.
28259765	0	55	theme	Compound	45:52	arg1	Kinetics					25:32	the Transport Kinetics	11:32	the Transport Kinetics of a Model Compound Released From Cellulosic Coacervate Compositions Into Artificial Sebum	11:123	Evaluating the Transport Kinetics of a Model Compound Released From Cellulosic Coacervate Compositions Into Artificial Sebum.
28259765	5	56	theme	wt	887:888	arg1	water					891:895	wt% water	887:895	90 to 50 wt% water	878:895	Tested compositions ranged from 90 to 50 wt% water and had a cat-HECDS to NaDS wt% ratio of 2:1, 1:1, or 1:2, mimicking the in vivo hydration range and relative excess surfactant content expected from commercial rinse-off formulations.
28259765	5	57	theme	rinse-off	1058:1066	arg1	formulations					1068:1079	commercial rinse-off formulations	1047:1079	commercial rinse-off formulations	1047:1079	Tested compositions ranged from 90 to 50 wt% water and had a cat-HECDS to NaDS wt% ratio of 2:1, 1:1, or 1:2, mimicking the in vivo hydration range and relative excess surfactant content expected from commercial rinse-off formulations.
28259765	6	58	theme	composition	1199:1209	arg1	content					1184:1190	water content	1178:1190	water content of the composition	1178:1209	Steady-state flux of the model compound from each coacervate composition was found to vary with water content of the composition.
28259765	6	59	theme	model	1107:1111	arg1	compound					1113:1120	the model compound	1103:1120	the model compound	1103:1120	Steady-state flux of the model compound from each coacervate composition was found to vary with water content of the composition.
28259765	4	60	theme	cationic-hydroxyethyl	743:763	arg1	cat-HECDS					792:800	cat-HECDS	792:800	cat-HECDS	792:800	Coacervate compositions were prepared with cationic-hydroxyethyl cellulose dodecyl sulfate (cat-HECDS), sodium dodecyl sulfate (NaDS), and water.
28259765	4	60	theme	cationic-hydroxyethyl	743:763	arg1	sulfate					783:789	cationic-hydroxyethyl cellulose dodecyl sulfate	743:789	cationic-hydroxyethyl cellulose dodecyl sulfate (cat-HECDS)	743:801	Coacervate compositions were prepared with cationic-hydroxyethyl cellulose dodecyl sulfate (cat-HECDS), sodium dodecyl sulfate (NaDS), and water.
28259765	0	61	theme	Model	39:43	arg1	Compound					45:52	a Model Compound	37:52	a Model Compound Released From Cellulosic Coacervate Compositions Into Artificial Sebum	37:123	Evaluating the Transport Kinetics of a Model Compound Released From Cellulosic Coacervate Compositions Into Artificial Sebum.
28259765	5	62	theme	relative	998:1005	arg1	content					1025:1031	relative excess surfactant content	998:1031	relative excess surfactant content	998:1031	Tested compositions ranged from 90 to 50 wt% water and had a cat-HECDS to NaDS wt% ratio of 2:1, 1:1, or 1:2, mimicking the in vivo hydration range and relative excess surfactant content expected from commercial rinse-off formulations.
28259765	5	63	dep	50	884:885	arg1	to					881:882	to	881:882	to	881:882	Tested compositions ranged from 90 to 50 wt% water and had a cat-HECDS to NaDS wt% ratio of 2:1, 1:1, or 1:2, mimicking the in vivo hydration range and relative excess surfactant content expected from commercial rinse-off formulations.
28259765	1	64	theme	Complex	126:132	arg1	coacervates					134:144	Complex coacervates	126:144	Complex coacervates	126:144	Complex coacervates of cationic polymers and anionic surfactants, which are produced spontaneously during the use of rinse-off formulations, represent an important delivery vehicle for topical agents to the skin surface and appendages.
28259765	1	65	theme	skin	333:336	arg1	surface					338:344	the skin surface	329:344	the skin surface	329:344	Complex coacervates of cationic polymers and anionic surfactants, which are produced spontaneously during the use of rinse-off formulations, represent an important delivery vehicle for topical agents to the skin surface and appendages.
28259765	5	66	theme	hydration	978:986	arg1	range					988:992	in vivo hydration range	970:992	in vivo hydration range	970:992	Tested compositions ranged from 90 to 50 wt% water and had a cat-HECDS to NaDS wt% ratio of 2:1, 1:1, or 1:2, mimicking the in vivo hydration range and relative excess surfactant content expected from commercial rinse-off formulations.
28259765	5	67	theme	excess	1007:1012	arg1	content					1025:1031	relative excess surfactant content	998:1031	relative excess surfactant content	998:1031	Tested compositions ranged from 90 to 50 wt% water and had a cat-HECDS to NaDS wt% ratio of 2:1, 1:1, or 1:2, mimicking the in vivo hydration range and relative excess surfactant content expected from commercial rinse-off formulations.
28259765	4	68	theme	Coacervate	700:709	arg1	compositions					711:722	Coacervate compositions	700:722	Coacervate compositions	700:722	Coacervate compositions were prepared with cationic-hydroxyethyl cellulose dodecyl sulfate (cat-HECDS), sodium dodecyl sulfate (NaDS), and water.
28259765	4	69	theme	dodecyl	775:781	arg1	cat-HECDS					792:800	cat-HECDS	792:800	cat-HECDS	792:800	Coacervate compositions were prepared with cationic-hydroxyethyl cellulose dodecyl sulfate (cat-HECDS), sodium dodecyl sulfate (NaDS), and water.
28259765	4	69	theme	dodecyl	775:781	arg1	sulfate					783:789	cationic-hydroxyethyl cellulose dodecyl sulfate	743:789	cationic-hydroxyethyl cellulose dodecyl sulfate (cat-HECDS)	743:801	Coacervate compositions were prepared with cationic-hydroxyethyl cellulose dodecyl sulfate (cat-HECDS), sodium dodecyl sulfate (NaDS), and water.
28259765	5	70	theme	surfactant	1014:1023	arg1	content					1025:1031	relative excess surfactant content	998:1031	relative excess surfactant content	998:1031	Tested compositions ranged from 90 to 50 wt% water and had a cat-HECDS to NaDS wt% ratio of 2:1, 1:1, or 1:2, mimicking the in vivo hydration range and relative excess surfactant content expected from commercial rinse-off formulations.
28259765	5	71	dep	NaDS	920:923	arg1	1:1					943:945	1:1	943:945	1:1	943:945	Tested compositions ranged from 90 to 50 wt% water and had a cat-HECDS to NaDS wt% ratio of 2:1, 1:1, or 1:2, mimicking the in vivo hydration range and relative excess surfactant content expected from commercial rinse-off formulations.
28259765	5	71	dep	NaDS	920:923	arg1	1:2					951:953	1:2	951:953	1:2	951:953	Tested compositions ranged from 90 to 50 wt% water and had a cat-HECDS to NaDS wt% ratio of 2:1, 1:1, or 1:2, mimicking the in vivo hydration range and relative excess surfactant content expected from commercial rinse-off formulations.
28259765	5	71	dep	NaDS	920:923	arg1	ratio					929:933	wt% ratio	925:933	wt% ratio of 2:1	925:940	Tested compositions ranged from 90 to 50 wt% water and had a cat-HECDS to NaDS wt% ratio of 2:1, 1:1, or 1:2, mimicking the in vivo hydration range and relative excess surfactant content expected from commercial rinse-off formulations.
28259765	4	72	theme	cellulose	765:773	arg1	cat-HECDS					792:800	cat-HECDS	792:800	cat-HECDS	792:800	Coacervate compositions were prepared with cationic-hydroxyethyl cellulose dodecyl sulfate (cat-HECDS), sodium dodecyl sulfate (NaDS), and water.
28259765	4	72	theme	cellulose	765:773	arg1	sulfate					783:789	cationic-hydroxyethyl cellulose dodecyl sulfate	743:789	cationic-hydroxyethyl cellulose dodecyl sulfate (cat-HECDS)	743:801	Coacervate compositions were prepared with cationic-hydroxyethyl cellulose dodecyl sulfate (cat-HECDS), sodium dodecyl sulfate (NaDS), and water.
28259765	2	73	theme	in vitro	514:521	arg1	testing					531:537	in vitro release testing	514:537	in vitro release testing	514:537	In this study, an artificial sebum-loaded cell culture insert method for determining the sebum diffusion properties of topical agents was optimized for in vitro release testing.
28259765	2	74	theme	culture	409:415	arg1	method					424:429	an artificial sebum-loaded cell culture insert method	377:429	an artificial sebum-loaded cell culture insert method for determining the sebum diffusion properties of topical agents	377:494	In this study, an artificial sebum-loaded cell culture insert method for determining the sebum diffusion properties of topical agents was optimized for in vitro release testing.
28259765	3	75	theme	compound	620:627	arg1	kinetics					600:607	the transport kinetics	586:607	the transport kinetics of a model compound, kinetin, released from semi-solid coacervate formulations into sebum	586:697	This method was subsequently used to evaluate the transport kinetics of a model compound, kinetin, released from semi-solid coacervate formulations into sebum.
28259765	5	76	contain	had	901:903	arg1	compositions					853:864	Tested compositions	846:864	Tested compositions	846:864	Tested compositions ranged from 90 to 50 wt% water and had a cat-HECDS to NaDS wt% ratio of 2:1, 1:1, or 1:2, mimicking the in vivo hydration range and relative excess surfactant content expected from commercial rinse-off formulations.
28259765	5	76	contain	had	901:903	arg2	cat-HECDS					907:915	a cat-HECDS	905:915	a cat-HECDS	905:915	Tested compositions ranged from 90 to 50 wt% water and had a cat-HECDS to NaDS wt% ratio of 2:1, 1:1, or 1:2, mimicking the in vivo hydration range and relative excess surfactant content expected from commercial rinse-off formulations.
28259765	1	77	theme	cationic	149:156	arg1	polymers					158:165	cationic polymers	149:165	cationic polymers	149:165	Complex coacervates of cationic polymers and anionic surfactants, which are produced spontaneously during the use of rinse-off formulations, represent an important delivery vehicle for topical agents to the skin surface and appendages.
28259765	1	78	theme	rinse-off	243:251	arg1	formulations					253:264	rinse-off formulations	243:264	rinse-off formulations	243:264	Complex coacervates of cationic polymers and anionic surfactants, which are produced spontaneously during the use of rinse-off formulations, represent an important delivery vehicle for topical agents to the skin surface and appendages.
28259765	8	79	theme	relevant	1430:1437	arg1	differences					1439:1449	clinically relevant differences	1419:1449	clinically relevant differences in transport kinetics from different coacervate formulations using a practical sample size	1419:1540	The in vitro release testing method proved capable of discriminating between clinically relevant differences in transport kinetics from different coacervate formulations using a practical sample size.
28259765	5	80	theme	Tested	846:851	arg1	compositions					853:864	Tested compositions	846:864	Tested compositions	846:864	Tested compositions ranged from 90 to 50 wt% water and had a cat-HECDS to NaDS wt% ratio of 2:1, 1:1, or 1:2, mimicking the in vivo hydration range and relative excess surfactant content expected from commercial rinse-off formulations.
28259765	1	81	theme	polymers	158:165	arg1	coacervates					134:144	Complex coacervates	126:144	Complex coacervates	126:144	Complex coacervates of cationic polymers and anionic surfactants, which are produced spontaneously during the use of rinse-off formulations, represent an important delivery vehicle for topical agents to the skin surface and appendages.
28259765	1	82	theme	formulations	253:264	arg1	use					236:238	the use	232:238	the use of rinse-off formulations	232:264	Complex coacervates of cationic polymers and anionic surfactants, which are produced spontaneously during the use of rinse-off formulations, represent an important delivery vehicle for topical agents to the skin surface and appendages.
27702528	0	0	theme	bacterium	72:80	arg1	miurensis					100:108	a salt pan bacterium Halolactibacillus miurensis	61:108	a salt pan bacterium Halolactibacillus miurensis	61:108	In vitro antioxidant activities of an exopolysaccharide from a salt pan bacterium Halolactibacillus miurensis.
27702528	8	1	theme	total	1034:1038	arg1	assay					1052:1056	the total antioxidant assay	1030:1056	the total antioxidant assay	1030:1056	10mg/ml of HMEPS was found equivalent to 2.7units of ascorbic acid through the total antioxidant assay.
27702528	8	2	theme	HMEPS	966:970	arg1	10mg/ml					955:961	10mg/ml	955:961	10mg/ml of HMEPS	955:970	10mg/ml of HMEPS was found equivalent to 2.7units of ascorbic acid through the total antioxidant assay.
27702528	9	3	theme	Phylogenetic	1059:1070	arg1	relation					1072:1079	Phylogenetic relation	1059:1079	Phylogenetic relation of H. miurensis SEEN MKU3 (GenBank number KT803852)	1059:1131	Phylogenetic relation of H. miurensis SEEN MKU3 (GenBank number KT803852) was plotted with MEGA 5.0.
27702528	0	4	theme	pan	68:70	arg1	miurensis					100:108	a salt pan bacterium Halolactibacillus miurensis	61:108	a salt pan bacterium Halolactibacillus miurensis	61:108	In vitro antioxidant activities of an exopolysaccharide from a salt pan bacterium Halolactibacillus miurensis.
27702528	5	5	theme	fingerprint	643:653	arg1	region					655:660	the fingerprint region	639:660	the fingerprint region	639:660	FT-IR spectra of the HMEPS revealed its composition with hydroxyl, alkenes, amide and carboxyl as functional groups and aliphatic amine and alkynes at the fingerprint region.
27702528	5	6	theme	functional	586:595	arg1	composition					528:538	its composition	524:538	its composition	524:538	FT-IR spectra of the HMEPS revealed its composition with hydroxyl, alkenes, amide and carboxyl as functional groups and aliphatic amine and alkynes at the fingerprint region.
27702528	5	6	theme	functional	586:595	arg1	groups					597:602	functional groups	586:602	functional groups	586:602	FT-IR spectra of the HMEPS revealed its composition with hydroxyl, alkenes, amide and carboxyl as functional groups and aliphatic amine and alkynes at the fingerprint region.
27702528	6	7	dep	In	663:664	arg1	vitro					666:670	vitro	666:670	vitro	666:670	In vitro antioxidant activity was investigated against hydroxyl, DPPH, superoxide free radicals and the scavenging activity against all were found to be dose dependent proportionately.
27702528	2	8	theme	total	304:308	arg1	content					323:329	a 56.1% total carbohydrate content	296:329	a 56.1% total carbohydrate content	296:329	EPS extracted from the supernatant yielded a 56.1% total carbohydrate content.
27702528	0	9	theme	Halolactibacillus	82:98	arg1	miurensis					100:108	a salt pan bacterium Halolactibacillus miurensis	61:108	a salt pan bacterium Halolactibacillus miurensis	61:108	In vitro antioxidant activities of an exopolysaccharide from a salt pan bacterium Halolactibacillus miurensis.
27702528	8	10	theme	antioxidant	1040:1050	arg1	assay					1052:1056	the total antioxidant assay	1030:1056	the total antioxidant assay	1030:1056	10mg/ml of HMEPS was found equivalent to 2.7units of ascorbic acid through the total antioxidant assay.
27702528	2	11	theme	%	302:302	arg1	content					323:329	a 56.1% total carbohydrate content	296:329	a 56.1% total carbohydrate content	296:329	EPS extracted from the supernatant yielded a 56.1% total carbohydrate content.
27702528	6	12	theme	hydroxyl	718:725	arg1	radicals					750:757	hydroxyl, DPPH, superoxide free radicals	718:757	radicals	750:757	In vitro antioxidant activity was investigated against hydroxyl, DPPH, superoxide free radicals and the scavenging activity against all were found to be dose dependent proportionately.
27702528	9	13	theme	MKU3	1102:1105	arg1	relation					1072:1079	Phylogenetic relation	1059:1079	Phylogenetic relation of H. miurensis SEEN MKU3 (GenBank number KT803852)	1059:1131	Phylogenetic relation of H. miurensis SEEN MKU3 (GenBank number KT803852) was plotted with MEGA 5.0.
27702528	4	14	theme	HPLC	473:476	arg1	analysis					478:485	HPLC analysis	473:485	HPLC analysis	473:485	Galactose and glucose were found as main monosaccharides of the HMEPS through HPLC analysis.
27702528	5	15	theme	aliphatic	608:616	arg1	amine					618:622	aliphatic amine	608:622	aliphatic amine	608:622	FT-IR spectra of the HMEPS revealed its composition with hydroxyl, alkenes, amide and carboxyl as functional groups and aliphatic amine and alkynes at the fingerprint region.
27702528	2	16	theme	56.1	298:301	arg1	%					302:302	%	302:302	%	302:302	EPS extracted from the supernatant yielded a 56.1% total carbohydrate content.
27702528	7	17	theme	reducing	868:875	arg1	ability					877:883	higher reducing ability	861:883	higher reducing ability	861:883	HMEPS showed higher reducing ability against superoxide radical and potency in chelating the ferrous ions.
27702528	1	18	attach	isolated	159:166	arg2	Exopolysaccharide					111:127	Exopolysaccharide	111:127	Exopolysaccharide (EPS) designated as HMEPS	111:153	Exopolysaccharide (EPS) designated as HMEPS was isolated from Halolactibacillus miurensis and purified through gel filtration chromatography.
27702528	1	18	attach	isolated	159:166	arg1	miurensis					191:199	Halolactibacillus miurensis	173:199	Halolactibacillus miurensis	173:199	Exopolysaccharide (EPS) designated as HMEPS was isolated from Halolactibacillus miurensis and purified through gel filtration chromatography.
27702528	1	18	attach	isolated	159:166	arg2	EPS					130:132	EPS	130:132	EPS	130:132	Exopolysaccharide (EPS) designated as HMEPS was isolated from Halolactibacillus miurensis and purified through gel filtration chromatography.
27702528	8	19	theme	ascorbic	1008:1015	arg1	acid					1017:1020	ascorbic acid	1008:1020	ascorbic acid	1008:1020	10mg/ml of HMEPS was found equivalent to 2.7units of ascorbic acid through the total antioxidant assay.
27702528	9	20	theme	SEEN	1097:1100	arg1	MKU3					1102:1105	H. miurensis SEEN MKU3	1084:1105	H. miurensis SEEN MKU3 (GenBank number KT803852)	1084:1131	Phylogenetic relation of H. miurensis SEEN MKU3 (GenBank number KT803852) was plotted with MEGA 5.0.
27702528	9	20	theme	SEEN	1097:1100	arg1	number					1116:1121	GenBank number KT803852	1108:1130	GenBank number KT803852	1108:1130	Phylogenetic relation of H. miurensis SEEN MKU3 (GenBank number KT803852) was plotted with MEGA 5.0.
27702528	1	21	theme	Halolactibacillus	173:189	arg1	miurensis					191:199	Halolactibacillus miurensis	173:199	Halolactibacillus miurensis	173:199	Exopolysaccharide (EPS) designated as HMEPS was isolated from Halolactibacillus miurensis and purified through gel filtration chromatography.
27702528	0	22	theme	salt	63:66	arg1	miurensis					100:108	a salt pan bacterium Halolactibacillus miurensis	61:108	a salt pan bacterium Halolactibacillus miurensis	61:108	In vitro antioxidant activities of an exopolysaccharide from a salt pan bacterium Halolactibacillus miurensis.
27702528	5	23	theme	HMEPS	509:513	arg1	spectra					494:500	FT-IR spectra	488:500	FT-IR spectra of the HMEPS	488:513	FT-IR spectra of the HMEPS revealed its composition with hydroxyl, alkenes, amide and carboxyl as functional groups and aliphatic amine and alkynes at the fingerprint region.
27702528	0	24	theme	In	0:1	arg1	activities					21:30	In vitro antioxidant activities	0:30	In vitro antioxidant activities of an exopolysaccharide from a salt pan bacterium Halolactibacillus miurensis.	0:109	In vitro antioxidant activities of an exopolysaccharide from a salt pan bacterium Halolactibacillus miurensis.
27702528	6	25	theme	In	663:664	arg1	activity					684:691	In vitro antioxidant activity	663:691	In vitro antioxidant activity	663:691	In vitro antioxidant activity was investigated against hydroxyl, DPPH, superoxide free radicals and the scavenging activity against all were found to be dose dependent proportionately.
27702528	6	26	theme	scavenging	767:776	arg1	activity					778:785	the scavenging activity	763:785	the scavenging activity against all	763:797	In vitro antioxidant activity was investigated against hydroxyl, DPPH, superoxide free radicals and the scavenging activity against all were found to be dose dependent proportionately.
27702528	6	26	theme	scavenging	767:776	arg1	dependent					821:829	dependent	821:829	dependent	821:829	In vitro antioxidant activity was investigated against hydroxyl, DPPH, superoxide free radicals and the scavenging activity against all were found to be dose dependent proportionately.
27702528	6	27	theme	antioxidant	672:682	arg1	activity					684:691	In vitro antioxidant activity	663:691	In vitro antioxidant activity	663:691	In vitro antioxidant activity was investigated against hydroxyl, DPPH, superoxide free radicals and the scavenging activity against all were found to be dose dependent proportionately.
27702528	0	28	theme	antioxidant	9:19	arg1	activities					21:30	In vitro antioxidant activities	0:30	In vitro antioxidant activities of an exopolysaccharide from a salt pan bacterium Halolactibacillus miurensis.	0:109	In vitro antioxidant activities of an exopolysaccharide from a salt pan bacterium Halolactibacillus miurensis.
27702528	2	29	theme	carbohydrate	310:321	arg1	content					323:329	a 56.1% total carbohydrate content	296:329	a 56.1% total carbohydrate content	296:329	EPS extracted from the supernatant yielded a 56.1% total carbohydrate content.
27702528	5	30	theme	FT-IR	488:492	arg1	spectra					494:500	FT-IR spectra	488:500	FT-IR spectra of the HMEPS	488:513	FT-IR spectra of the HMEPS revealed its composition with hydroxyl, alkenes, amide and carboxyl as functional groups and aliphatic amine and alkynes at the fingerprint region.
27702528	1	31	theme	gel	222:224	arg1	chromatography					237:250	gel filtration chromatography	222:250	gel filtration chromatography	222:250	Exopolysaccharide (EPS) designated as HMEPS was isolated from Halolactibacillus miurensis and purified through gel filtration chromatography.
27702528	5	32	from	region	655:660	arg1	alkynes					628:634	alkynes	628:634	alkynes at the fingerprint region	628:660	FT-IR spectra of the HMEPS revealed its composition with hydroxyl, alkenes, amide and carboxyl as functional groups and aliphatic amine and alkynes at the fingerprint region.
27702528	5	32	from	region	655:660	arg1	groups					597:602	functional groups	586:602	functional groups	586:602	FT-IR spectra of the HMEPS revealed its composition with hydroxyl, alkenes, amide and carboxyl as functional groups and aliphatic amine and alkynes at the fingerprint region.
27702528	5	32	from	region	655:660	arg1	amine					618:622	aliphatic amine	608:622	aliphatic amine	608:622	FT-IR spectra of the HMEPS revealed its composition with hydroxyl, alkenes, amide and carboxyl as functional groups and aliphatic amine and alkynes at the fingerprint region.
27702528	5	32	from	region	655:660	arg1	composition					528:538	its composition	524:538	its composition	524:538	FT-IR spectra of the HMEPS revealed its composition with hydroxyl, alkenes, amide and carboxyl as functional groups and aliphatic amine and alkynes at the fingerprint region.
27702528	8	33	theme	acid	1017:1020	arg1	2.7units					996:1003	2.7units	996:1003	2.7units of ascorbic acid through the total antioxidant assay	996:1056	10mg/ml of HMEPS was found equivalent to 2.7units of ascorbic acid through the total antioxidant assay.
27702528	7	34	theme	higher	861:866	arg1	ability					877:883	higher reducing ability	861:883	higher reducing ability	861:883	HMEPS showed higher reducing ability against superoxide radical and potency in chelating the ferrous ions.
27702528	1	35	theme	filtration	226:235	arg1	chromatography					237:250	gel filtration chromatography	222:250	gel filtration chromatography	222:250	Exopolysaccharide (EPS) designated as HMEPS was isolated from Halolactibacillus miurensis and purified through gel filtration chromatography.
27702528	4	36	theme	HMEPS	459:463	arg1	monosaccharides					436:450	main monosaccharides	431:450	main monosaccharides of the HMEPS	431:463	Galactose and glucose were found as main monosaccharides of the HMEPS through HPLC analysis.
27702528	4	36	theme	HMEPS	459:463	arg1	glucose					409:415	glucose	409:415	glucose	409:415	Galactose and glucose were found as main monosaccharides of the HMEPS through HPLC analysis.
27702528	4	36	theme	HMEPS	459:463	arg1	Galactose					395:403	Galactose	395:403	Galactose	395:403	Galactose and glucose were found as main monosaccharides of the HMEPS through HPLC analysis.
27702528	0	37	theme	exopolysaccharide	38:54	arg1	activities					21:30	In vitro antioxidant activities	0:30	In vitro antioxidant activities of an exopolysaccharide from a salt pan bacterium Halolactibacillus miurensis.	0:109	In vitro antioxidant activities of an exopolysaccharide from a salt pan bacterium Halolactibacillus miurensis.
27702528	9	38	dep	H.	1084:1085	arg1	miurensis					1087:1095	H. miurensis	1084:1095	H. miurensis	1084:1095	Phylogenetic relation of H. miurensis SEEN MKU3 (GenBank number KT803852) was plotted with MEGA 5.0.
27702528	7	39	theme	ferrous	941:947	arg1	ions					949:952	the ferrous ions	937:952	the ferrous ions	937:952	HMEPS showed higher reducing ability against superoxide radical and potency in chelating the ferrous ions.
27702528	6	40	theme	free	745:748	arg1	radicals					750:757	hydroxyl, DPPH, superoxide free radicals	718:757	radicals	750:757	In vitro antioxidant activity was investigated against hydroxyl, DPPH, superoxide free radicals and the scavenging activity against all were found to be dose dependent proportionately.
27702528	0	41	from	miurensis	100:108	arg1	activities					21:30	In vitro antioxidant activities	0:30	In vitro antioxidant activities of an exopolysaccharide from a salt pan bacterium Halolactibacillus miurensis.	0:109	In vitro antioxidant activities of an exopolysaccharide from a salt pan bacterium Halolactibacillus miurensis.
27702528	0	41	from	miurensis	100:108	arg1	exopolysaccharide					38:54	an exopolysaccharide	35:54	an exopolysaccharide from a salt pan bacterium Halolactibacillus miurensis	35:108	In vitro antioxidant activities of an exopolysaccharide from a salt pan bacterium Halolactibacillus miurensis.
27702528	9	42	dep	SEEN	1097:1100	arg1	H.					1084:1085	H.	1084:1085	H.	1084:1085	Phylogenetic relation of H. miurensis SEEN MKU3 (GenBank number KT803852) was plotted with MEGA 5.0.
27702528	6	43	theme	superoxide	734:743	arg1	radicals					750:757	hydroxyl, DPPH, superoxide free radicals	718:757	radicals	750:757	In vitro antioxidant activity was investigated against hydroxyl, DPPH, superoxide free radicals and the scavenging activity against all were found to be dose dependent proportionately.
27702528	9	44	theme	GenBank	1108:1114	arg1	MKU3					1102:1105	H. miurensis SEEN MKU3	1084:1105	H. miurensis SEEN MKU3 (GenBank number KT803852)	1084:1131	Phylogenetic relation of H. miurensis SEEN MKU3 (GenBank number KT803852) was plotted with MEGA 5.0.
27702528	9	44	theme	GenBank	1108:1114	arg1	number					1116:1121	GenBank number KT803852	1108:1130	GenBank number KT803852	1108:1130	Phylogenetic relation of H. miurensis SEEN MKU3 (GenBank number KT803852) was plotted with MEGA 5.0.
27702528	3	45	theme	moisture	344:351	arg1	content					353:359	moisture content	344:359	moisture content	344:359	The ash and moisture content were 12.1% and 1.5% respectively.
27702528	4	46	theme	main	431:434	arg1	monosaccharides					436:450	main monosaccharides	431:450	main monosaccharides of the HMEPS	431:463	Galactose and glucose were found as main monosaccharides of the HMEPS through HPLC analysis.
27702528	4	46	theme	main	431:434	arg1	glucose					409:415	glucose	409:415	glucose	409:415	Galactose and glucose were found as main monosaccharides of the HMEPS through HPLC analysis.
27702528	4	46	theme	main	431:434	arg1	Galactose					395:403	Galactose	395:403	Galactose	395:403	Galactose and glucose were found as main monosaccharides of the HMEPS through HPLC analysis.
27702528	7	47	theme	superoxide	893:902	arg1	radical					904:910	superoxide radical	893:910	superoxide radical	893:910	HMEPS showed higher reducing ability against superoxide radical and potency in chelating the ferrous ions.
27702528	0	48	from	activities	21:30	arg1	miurensis					100:108	a salt pan bacterium Halolactibacillus miurensis	61:108	a salt pan bacterium Halolactibacillus miurensis	61:108	In vitro antioxidant activities of an exopolysaccharide from a salt pan bacterium Halolactibacillus miurensis.
27702528	0	49	dep	In	0:1	arg1	vitro					3:7	vitro	3:7	vitro	3:7	In vitro antioxidant activities of an exopolysaccharide from a salt pan bacterium Halolactibacillus miurensis.
27702528	6	50	dep	radicals	750:757	arg1	DPPH					728:731	hydroxyl, DPPH, superoxide free radicals	718:757	DPPH	728:731	In vitro antioxidant activity was investigated against hydroxyl, DPPH, superoxide free radicals and the scavenging activity against all were found to be dose dependent proportionately.
28431331	5	0	from	elements	653:660	arg1	OMC					669:671	the OMC	665:671	the OMC	665:671	These processes can be traced to structural elements in the OMC and are influenced by the melting point of coconut oil, an essential element in the formulation.
28431331	2	1	dep	self-orders	256:266	arg1	amenable					315:322	amenable	315:322	amenable	315:322	This composition self-orders to a multi hierarchal structure and as such is amenable to be studied by techniques such as DS.
28431331	5	2	theme	coconut	716:722	arg1	oil					724:726	coconut oil	716:726	coconut oil	716:726	These processes can be traced to structural elements in the OMC and are influenced by the melting point of coconut oil, an essential element in the formulation.
28431331	5	2	theme	coconut	716:722	arg1	element					742:748	an essential element	729:748	an essential element in the formulation	729:767	These processes can be traced to structural elements in the OMC and are influenced by the melting point of coconut oil, an essential element in the formulation.
28431331	1	3	theme	matrix	133:138	arg1	OMC					149:151	OMC	149:151	OMC	149:151	Dielectric Spectroscopy (DS) was employed to study a novel oral matrix carrier (OMC), composed from silica nano-particles, polysaccharides, biopolymers and natural oils.
28431331	1	3	theme	matrix	133:138	arg1	carrier					140:146	a novel oral matrix carrier	120:146	a novel oral matrix carrier (OMC)	120:152	Dielectric Spectroscopy (DS) was employed to study a novel oral matrix carrier (OMC), composed from silica nano-particles, polysaccharides, biopolymers and natural oils.
28431331	4	4	theme	CC	537:538	arg1	relaxation					540:549	CC relaxation	537:549	CC relaxation	537:549	The results were dominated by two relaxation processes following CC relaxation and having relaxation times that are Arrhenius in nature.
28431331	1	5	theme	natural	225:231	arg1	oils					233:236	natural oils	225:236	natural oils	225:236	Dielectric Spectroscopy (DS) was employed to study a novel oral matrix carrier (OMC), composed from silica nano-particles, polysaccharides, biopolymers and natural oils.
28431331	1	6	theme	oral	128:131	arg1	OMC					149:151	OMC	149:151	OMC	149:151	Dielectric Spectroscopy (DS) was employed to study a novel oral matrix carrier (OMC), composed from silica nano-particles, polysaccharides, biopolymers and natural oils.
28431331	1	6	theme	oral	128:131	arg1	carrier					140:146	a novel oral matrix carrier	120:146	a novel oral matrix carrier (OMC)	120:152	Dielectric Spectroscopy (DS) was employed to study a novel oral matrix carrier (OMC), composed from silica nano-particles, polysaccharides, biopolymers and natural oils.
28431331	4	7	dep	processes	517:525	arg1	having					555:560	having	555:560	having relaxation times that are Arrhenius in nature	555:606	The results were dominated by two relaxation processes following CC relaxation and having relaxation times that are Arrhenius in nature.
28431331	4	7	dep	processes	517:525	arg1	following					527:535	following	527:535	following CC relaxation	527:549	The results were dominated by two relaxation processes following CC relaxation and having relaxation times that are Arrhenius in nature.
28431331	5	8	theme	essential	732:740	arg1	oil					724:726	coconut oil	716:726	coconut oil	716:726	These processes can be traced to structural elements in the OMC and are influenced by the melting point of coconut oil, an essential element in the formulation.
28431331	5	8	theme	essential	732:740	arg1	element					742:748	an essential element	729:748	an essential element in the formulation	729:767	These processes can be traced to structural elements in the OMC and are influenced by the melting point of coconut oil, an essential element in the formulation.
28431331	5	9	from	element	742:748	arg1	formulation					757:767	the formulation	753:767	the formulation	753:767	These processes can be traced to structural elements in the OMC and are influenced by the melting point of coconut oil, an essential element in the formulation.
28431331	5	10	theme	oil	724:726	arg1	point					707:711	the melting point	695:711	the melting point of coconut oil, an essential element in the formulation	695:767	These processes can be traced to structural elements in the OMC and are influenced by the melting point of coconut oil, an essential element in the formulation.
28431331	1	11	theme	Dielectric	69:78	arg1	DS					94:95	DS	94:95	DS	94:95	Dielectric Spectroscopy (DS) was employed to study a novel oral matrix carrier (OMC), composed from silica nano-particles, polysaccharides, biopolymers and natural oils.
28431331	1	11	theme	Dielectric	69:78	arg1	Spectroscopy					80:91	Dielectric Spectroscopy	69:91	Dielectric Spectroscopy (DS)	69:96	Dielectric Spectroscopy (DS) was employed to study a novel oral matrix carrier (OMC), composed from silica nano-particles, polysaccharides, biopolymers and natural oils.
28431331	5	12	theme	melting	699:705	arg1	point					707:711	the melting point	695:711	the melting point of coconut oil, an essential element in the formulation	695:767	These processes can be traced to structural elements in the OMC and are influenced by the melting point of coconut oil, an essential element in the formulation.
28431331	2	13	theme	hierarchal	279:288	arg1	structure					290:298	a multi hierarchal structure	271:298	a multi hierarchal structure	271:298	This composition self-orders to a multi hierarchal structure and as such is amenable to be studied by techniques such as DS.
28431331	3	14	theme	dielectric	368:377	arg1	properties					379:388	The dielectric properties	364:388	The dielectric properties	364:388	The dielectric properties were measured in the frequency range 1MHz-1GHz and a temperature range 10°C-45°C.
28431331	0	15	theme	Dielectric	0:9	arg1	properties					11:20	Dielectric properties	0:20	Dielectric properties of a novel colloidal oral matrix drug carrier	0:66	Dielectric properties of a novel colloidal oral matrix drug carrier.
28431331	4	16	theme	relaxation	506:515	arg1	processes					517:525	two relaxation processes	502:525	two relaxation processes following CC relaxation and having relaxation times that are Arrhenius in nature	502:606	The results were dominated by two relaxation processes following CC relaxation and having relaxation times that are Arrhenius in nature.
28431331	2	17	theme	multi	273:277	arg1	structure					290:298	a multi hierarchal structure	271:298	a multi hierarchal structure	271:298	This composition self-orders to a multi hierarchal structure and as such is amenable to be studied by techniques such as DS.
28431331	6	18	from	entities	816:823	arg1	OMC					832:834	the OMC	828:834	the OMC	828:834	Furthermore, the correlations between dipolar entities in the OMC are investigated using Froelich's B function formulation.
28431331	4	19	from	nature	601:606	arg1	Arrhenius					588:596	Arrhenius	588:596	Arrhenius	588:596	The results were dominated by two relaxation processes following CC relaxation and having relaxation times that are Arrhenius in nature.
28431331	3	20	theme	frequency	411:419	arg1	range					421:425	the frequency range 1MHz-1GHz and a temperature range 10°C-45°C	407:469	the frequency range 1MHz-1GHz and a temperature range 10°C-45°C	407:469	The dielectric properties were measured in the frequency range 1MHz-1GHz and a temperature range 10°C-45°C.
28431331	0	21	theme	colloidal	33:41	arg1	carrier					60:66	a novel colloidal oral matrix drug carrier	25:66	a novel colloidal oral matrix drug carrier	25:66	Dielectric properties of a novel colloidal oral matrix drug carrier.
28431331	6	22	theme	dipolar	808:814	arg1	entities					816:823	dipolar entities	808:823	dipolar entities in the OMC	808:834	Furthermore, the correlations between dipolar entities in the OMC are investigated using Froelich's B function formulation.
28431331	4	23	from	Arrhenius	588:596	arg1	nature					601:606	nature	601:606	nature	601:606	The results were dominated by two relaxation processes following CC relaxation and having relaxation times that are Arrhenius in nature.
28431331	0	24	theme	novel	27:31	arg1	carrier					60:66	a novel colloidal oral matrix drug carrier	25:66	a novel colloidal oral matrix drug carrier	25:66	Dielectric properties of a novel colloidal oral matrix drug carrier.
28431331	1	25	theme	silica	169:174	arg1	nano-particles					176:189	silica nano-particles	169:189	silica nano-particles	169:189	Dielectric Spectroscopy (DS) was employed to study a novel oral matrix carrier (OMC), composed from silica nano-particles, polysaccharides, biopolymers and natural oils.
28431331	0	26	theme	matrix	48:53	arg1	carrier					60:66	a novel colloidal oral matrix drug carrier	25:66	a novel colloidal oral matrix drug carrier	25:66	Dielectric properties of a novel colloidal oral matrix drug carrier.
28431331	7	27	theme	systems	962:968	arg1	study					947:951	the study	943:951	the study of these systems	943:968	The results point to DS as an effective tool for the study of these systems.
28431331	0	28	theme	oral	43:46	arg1	carrier					60:66	a novel colloidal oral matrix drug carrier	25:66	a novel colloidal oral matrix drug carrier	25:66	Dielectric properties of a novel colloidal oral matrix drug carrier.
28431331	6	29	theme	function	872:879	arg1	formulation					881:891	Froelich's B function formulation	859:891	Froelich's B function formulation	859:891	Furthermore, the correlations between dipolar entities in the OMC are investigated using Froelich's B function formulation.
28431331	3	30	theme	temperature	443:453	arg1	range					455:459	a temperature range 10°C-45°C	441:469	a temperature range 10°C-45°C	441:469	The dielectric properties were measured in the frequency range 1MHz-1GHz and a temperature range 10°C-45°C.
28431331	6	31	theme	B	870:870	arg1	formulation					881:891	Froelich's B function formulation	859:891	Froelich's B function formulation	859:891	Furthermore, the correlations between dipolar entities in the OMC are investigated using Froelich's B function formulation.
28431331	5	32	theme	structural	642:651	arg1	elements					653:660	structural elements	642:660	structural elements in the OMC	642:671	These processes can be traced to structural elements in the OMC and are influenced by the melting point of coconut oil, an essential element in the formulation.
28431331	4	33	theme	relaxation	562:571	arg1	times					573:577	relaxation times	562:577	relaxation times that are Arrhenius in nature	562:606	The results were dominated by two relaxation processes following CC relaxation and having relaxation times that are Arrhenius in nature.
28431331	0	34	theme	carrier	60:66	arg1	properties					11:20	Dielectric properties	0:20	Dielectric properties of a novel colloidal oral matrix drug carrier	0:66	Dielectric properties of a novel colloidal oral matrix drug carrier.
28431331	7	35	theme	effective	924:932	arg1	tool					934:937	an effective tool	921:937	an effective tool for the study of these systems	921:968	The results point to DS as an effective tool for the study of these systems.
28431331	0	36	theme	drug	55:58	arg1	carrier					60:66	a novel colloidal oral matrix drug carrier	25:66	a novel colloidal oral matrix drug carrier	25:66	Dielectric properties of a novel colloidal oral matrix drug carrier.
28431331	3	37	dep	range	421:425	arg1	1MHz-1GHz					427:435	1MHz-1GHz	427:435	1MHz-1GHz	427:435	The dielectric properties were measured in the frequency range 1MHz-1GHz and a temperature range 10°C-45°C.
28431331	3	37	dep	range	421:425	arg1	range					455:459	a temperature range 10°C-45°C	441:469	a temperature range 10°C-45°C	441:469	The dielectric properties were measured in the frequency range 1MHz-1GHz and a temperature range 10°C-45°C.
28431331	1	38	theme	novel	122:126	arg1	OMC					149:151	OMC	149:151	OMC	149:151	Dielectric Spectroscopy (DS) was employed to study a novel oral matrix carrier (OMC), composed from silica nano-particles, polysaccharides, biopolymers and natural oils.
28431331	1	38	theme	novel	122:126	arg1	carrier					140:146	a novel oral matrix carrier	120:146	a novel oral matrix carrier (OMC)	120:152	Dielectric Spectroscopy (DS) was employed to study a novel oral matrix carrier (OMC), composed from silica nano-particles, polysaccharides, biopolymers and natural oils.
30549892	0	0	theme	Tea	69:71	arg1	Extracts					35:42	Water Extracts	29:42	Water Extracts of Black, Green and Dark Tea	29:71	Vasculoprotective Effects of Water Extracts of Black, Green and Dark Tea in Vitro.
30549892	2	1	theme	colorimetric	398:409	arg1	methods					411:417	colorimetric methods	398:417	colorimetric methods	398:417	High Performance Liquid Chromatography (HPLC) and colorimetric methods were conducted to analyze for tea catechins, caffeine, polyphenols, amino acids and polysaccharides of BTE, GTE and DTE.
30549892	6	2	theme	hyperglycemia	1081:1093	arg1	progression					1066:1076	the progression	1062:1076	the progression of hyperglycemia	1062:1093	The results suggest that regular consumption of tea can help prevent the progression of hyperglycemia and the vascular complications of diabetes.
30549892	6	2	theme	hyperglycemia	1081:1093	arg1	complications					1112:1124	the vascular complications	1099:1124	the vascular complications of diabetes	1099:1136	The results suggest that regular consumption of tea can help prevent the progression of hyperglycemia and the vascular complications of diabetes.
30549892	5	3	theme	uptake	953:958	arg1	effect					970:975	the glucose uptake promotion effect	941:975	the glucose uptake promotion effect in HepG2 cells	941:990	BTE, GTE, and DTE showed the inhibitory effects of a-glucosidase, AR, and AGEs, but only DTE exhibited the glucose uptake promotion effect in HepG2 cells.
30549892	3	4	theme	advanced	604:611	arg1	AGEs					637:640	AGEs	637:640	AGEs	637:640	The inhibitory effects of α-glucosidase, aldose reductase (AR), advanced glycation end-products (AGEs) and glucose uptake promotion effect in BTE, GTE and DTE were explored in vitro.
30549892	3	4	theme	advanced	604:611	arg1	end-products					623:634	advanced glycation end-products	604:634	advanced glycation end-products (AGEs)	604:641	The inhibitory effects of α-glucosidase, aldose reductase (AR), advanced glycation end-products (AGEs) and glucose uptake promotion effect in BTE, GTE and DTE were explored in vitro.
30549892	0	5	theme	Dark	64:67	arg1	Tea					69:71	Black, Green and Dark Tea	47:71	Tea	69:71	Vasculoprotective Effects of Water Extracts of Black, Green and Dark Tea in Vitro.
30549892	1	6	theme	green	295:299	arg1	GTE					314:316	GTE	314:316	GTE	314:316	To investigate the preventive effects of tea on hyperglycemia and vascular complications of diabetes, we report the extraction and composition as well as the vasculoprotective effects of black tea extract (BTE), green tea extract (GTE), and dark tea extract (DTE).
30549892	1	6	theme	green	295:299	arg1	extract					305:311	green tea extract	295:311	green tea extract (GTE)	295:317	To investigate the preventive effects of tea on hyperglycemia and vascular complications of diabetes, we report the extraction and composition as well as the vasculoprotective effects of black tea extract (BTE), green tea extract (GTE), and dark tea extract (DTE).
30549892	5	7	from	effect	970:975	arg1	cells					986:990	HepG2 cells	980:990	HepG2 cells	980:990	BTE, GTE, and DTE showed the inhibitory effects of a-glucosidase, AR, and AGEs, but only DTE exhibited the glucose uptake promotion effect in HepG2 cells.
30549892	1	8	theme	tea	329:331	arg1	DTE					342:344	DTE	342:344	DTE	342:344	To investigate the preventive effects of tea on hyperglycemia and vascular complications of diabetes, we report the extraction and composition as well as the vasculoprotective effects of black tea extract (BTE), green tea extract (GTE), and dark tea extract (DTE).
30549892	1	8	theme	tea	329:331	arg1	extract					333:339	dark tea extract	324:339	dark tea extract (DTE)	324:345	To investigate the preventive effects of tea on hyperglycemia and vascular complications of diabetes, we report the extraction and composition as well as the vasculoprotective effects of black tea extract (BTE), green tea extract (GTE), and dark tea extract (DTE).
30549892	1	9	theme	tea	301:303	arg1	GTE					314:316	GTE	314:316	GTE	314:316	To investigate the preventive effects of tea on hyperglycemia and vascular complications of diabetes, we report the extraction and composition as well as the vasculoprotective effects of black tea extract (BTE), green tea extract (GTE), and dark tea extract (DTE).
30549892	1	9	theme	tea	301:303	arg1	extract					305:311	green tea extract	295:311	green tea extract (GTE)	295:317	To investigate the preventive effects of tea on hyperglycemia and vascular complications of diabetes, we report the extraction and composition as well as the vasculoprotective effects of black tea extract (BTE), green tea extract (GTE), and dark tea extract (DTE).
30549892	4	10	theme	major	739:743	arg1	forms					754:758	six major catechin forms	735:758	six major catechin forms	735:758	Contents of six major catechin forms and total catechin as well as polyphenols are higher in GTE and DTE than BTE.
30549892	3	11	dep	α-glucosidase	566:578	arg1	effect					672:677	promotion effect	662:677	promotion effect	662:677	The inhibitory effects of α-glucosidase, aldose reductase (AR), advanced glycation end-products (AGEs) and glucose uptake promotion effect in BTE, GTE and DTE were explored in vitro.
30549892	4	12	theme	polyphenols	790:800	arg1	Contents					723:730	Contents	723:730	Contents of six major catechin forms and total catechin as well as polyphenols	723:800	Contents of six major catechin forms and total catechin as well as polyphenols are higher in GTE and DTE than BTE.
30549892	1	13	theme	extract	305:311	arg1	composition					214:224	composition	214:224	composition	214:224	To investigate the preventive effects of tea on hyperglycemia and vascular complications of diabetes, we report the extraction and composition as well as the vasculoprotective effects of black tea extract (BTE), green tea extract (GTE), and dark tea extract (DTE).
30549892	1	13	theme	extract	305:311	arg1	extraction					199:208	extraction	199:208	extraction	199:208	To investigate the preventive effects of tea on hyperglycemia and vascular complications of diabetes, we report the extraction and composition as well as the vasculoprotective effects of black tea extract (BTE), green tea extract (GTE), and dark tea extract (DTE).
30549892	1	13	theme	extract	305:311	arg1	effects					259:265	the vasculoprotective effects	237:265	the extraction and composition as well as the vasculoprotective effects of black tea extract (BTE), green tea extract (GTE), and dark tea extract (DTE)	195:345	To investigate the preventive effects of tea on hyperglycemia and vascular complications of diabetes, we report the extraction and composition as well as the vasculoprotective effects of black tea extract (BTE), green tea extract (GTE), and dark tea extract (DTE).
30549892	3	14	theme	uptake	655:660	arg1	effects					555:561	The inhibitory effects	540:561	The inhibitory effects of α-glucosidase, aldose reductase (AR), advanced glycation end-products (AGEs) and glucose uptake promotion effect in BTE, GTE and DTE	540:697	The inhibitory effects of α-glucosidase, aldose reductase (AR), advanced glycation end-products (AGEs) and glucose uptake promotion effect in BTE, GTE and DTE were explored in vitro.
30549892	3	15	theme	glycation	613:621	arg1	AGEs					637:640	AGEs	637:640	AGEs	637:640	The inhibitory effects of α-glucosidase, aldose reductase (AR), advanced glycation end-products (AGEs) and glucose uptake promotion effect in BTE, GTE and DTE were explored in vitro.
30549892	3	15	theme	glycation	613:621	arg1	end-products					623:634	advanced glycation end-products	604:634	advanced glycation end-products (AGEs)	604:641	The inhibitory effects of α-glucosidase, aldose reductase (AR), advanced glycation end-products (AGEs) and glucose uptake promotion effect in BTE, GTE and DTE were explored in vitro.
30549892	2	16	theme	DTE	535:537	arg1	polysaccharides					503:517	polysaccharides	503:517	polysaccharides	503:517	High Performance Liquid Chromatography (HPLC) and colorimetric methods were conducted to analyze for tea catechins, caffeine, polyphenols, amino acids and polysaccharides of BTE, GTE and DTE.
30549892	2	16	theme	DTE	535:537	arg1	acids					493:497	amino acids	487:497	amino acids	487:497	High Performance Liquid Chromatography (HPLC) and colorimetric methods were conducted to analyze for tea catechins, caffeine, polyphenols, amino acids and polysaccharides of BTE, GTE and DTE.
30549892	2	16	theme	DTE	535:537	arg1	catechins					453:461	tea catechins	449:461	tea catechins	449:461	High Performance Liquid Chromatography (HPLC) and colorimetric methods were conducted to analyze for tea catechins, caffeine, polyphenols, amino acids and polysaccharides of BTE, GTE and DTE.
30549892	5	17	theme	AR	904:905	arg1	effects					878:884	the inhibitory effects	863:884	the inhibitory effects of a-glucosidase, AR, and AGEs	863:915	BTE, GTE, and DTE showed the inhibitory effects of a-glucosidase, AR, and AGEs, but only DTE exhibited the glucose uptake promotion effect in HepG2 cells.
30549892	1	18	dep	tea	276:278	arg1	extract					280:286	extract	280:286	extract (BTE)	280:292	To investigate the preventive effects of tea on hyperglycemia and vascular complications of diabetes, we report the extraction and composition as well as the vasculoprotective effects of black tea extract (BTE), green tea extract (GTE), and dark tea extract (DTE).
30549892	1	18	dep	tea	276:278	arg1	BTE					289:291	BTE	289:291	BTE	289:291	To investigate the preventive effects of tea on hyperglycemia and vascular complications of diabetes, we report the extraction and composition as well as the vasculoprotective effects of black tea extract (BTE), green tea extract (GTE), and dark tea extract (DTE).
30549892	2	19	theme	GTE	527:529	arg1	polysaccharides					503:517	polysaccharides	503:517	polysaccharides	503:517	High Performance Liquid Chromatography (HPLC) and colorimetric methods were conducted to analyze for tea catechins, caffeine, polyphenols, amino acids and polysaccharides of BTE, GTE and DTE.
30549892	2	19	theme	GTE	527:529	arg1	acids					493:497	amino acids	487:497	amino acids	487:497	High Performance Liquid Chromatography (HPLC) and colorimetric methods were conducted to analyze for tea catechins, caffeine, polyphenols, amino acids and polysaccharides of BTE, GTE and DTE.
30549892	2	19	theme	GTE	527:529	arg1	catechins					453:461	tea catechins	449:461	tea catechins	449:461	High Performance Liquid Chromatography (HPLC) and colorimetric methods were conducted to analyze for tea catechins, caffeine, polyphenols, amino acids and polysaccharides of BTE, GTE and DTE.
30549892	2	20	theme	Performance	353:363	arg1	HPLC					388:391	HPLC	388:391	HPLC	388:391	High Performance Liquid Chromatography (HPLC) and colorimetric methods were conducted to analyze for tea catechins, caffeine, polyphenols, amino acids and polysaccharides of BTE, GTE and DTE.
30549892	2	20	theme	Performance	353:363	arg1	Chromatography					372:385	High Performance Liquid Chromatography	348:385	High Performance Liquid Chromatography (HPLC)	348:392	High Performance Liquid Chromatography (HPLC) and colorimetric methods were conducted to analyze for tea catechins, caffeine, polyphenols, amino acids and polysaccharides of BTE, GTE and DTE.
30549892	1	21	theme	vascular	149:156	arg1	complications					158:170	vascular complications	149:170	vascular complications of diabetes	149:182	To investigate the preventive effects of tea on hyperglycemia and vascular complications of diabetes, we report the extraction and composition as well as the vasculoprotective effects of black tea extract (BTE), green tea extract (GTE), and dark tea extract (DTE).
30549892	2	22	theme	Liquid	365:370	arg1	HPLC					388:391	HPLC	388:391	HPLC	388:391	High Performance Liquid Chromatography (HPLC) and colorimetric methods were conducted to analyze for tea catechins, caffeine, polyphenols, amino acids and polysaccharides of BTE, GTE and DTE.
30549892	2	22	theme	Liquid	365:370	arg1	Chromatography					372:385	High Performance Liquid Chromatography	348:385	High Performance Liquid Chromatography (HPLC)	348:392	High Performance Liquid Chromatography (HPLC) and colorimetric methods were conducted to analyze for tea catechins, caffeine, polyphenols, amino acids and polysaccharides of BTE, GTE and DTE.
30549892	5	23	theme	HepG2	980:984	arg1	cells					986:990	HepG2 cells	980:990	HepG2 cells	980:990	BTE, GTE, and DTE showed the inhibitory effects of a-glucosidase, AR, and AGEs, but only DTE exhibited the glucose uptake promotion effect in HepG2 cells.
30549892	1	24	from	effects	113:119	arg1	complications					158:170	vascular complications	149:170	vascular complications of diabetes	149:182	To investigate the preventive effects of tea on hyperglycemia and vascular complications of diabetes, we report the extraction and composition as well as the vasculoprotective effects of black tea extract (BTE), green tea extract (GTE), and dark tea extract (DTE).
30549892	1	24	from	effects	113:119	arg1	hyperglycemia					131:143	hyperglycemia	131:143	hyperglycemia	131:143	To investigate the preventive effects of tea on hyperglycemia and vascular complications of diabetes, we report the extraction and composition as well as the vasculoprotective effects of black tea extract (BTE), green tea extract (GTE), and dark tea extract (DTE).
30549892	6	25	theme	vascular	1103:1110	arg1	complications					1112:1124	the vascular complications	1099:1124	the vascular complications of diabetes	1099:1136	The results suggest that regular consumption of tea can help prevent the progression of hyperglycemia and the vascular complications of diabetes.
30549892	3	26	theme	reductase	588:596	arg1	effects					555:561	The inhibitory effects	540:561	The inhibitory effects of α-glucosidase, aldose reductase (AR), advanced glycation end-products (AGEs) and glucose uptake promotion effect in BTE, GTE and DTE	540:697	The inhibitory effects of α-glucosidase, aldose reductase (AR), advanced glycation end-products (AGEs) and glucose uptake promotion effect in BTE, GTE and DTE were explored in vitro.
30549892	0	27	theme	Vasculoprotective	0:16	arg1	Effects					18:24	Vasculoprotective Effects	0:24	Vasculoprotective Effects of Water Extracts of Black, Green and Dark Tea in Vitro	0:80	Vasculoprotective Effects of Water Extracts of Black, Green and Dark Tea in Vitro.
30549892	1	28	theme	vasculoprotective	241:257	arg1	effects					259:265	the vasculoprotective effects	237:265	the extraction and composition as well as the vasculoprotective effects of black tea extract (BTE), green tea extract (GTE), and dark tea extract (DTE)	195:345	To investigate the preventive effects of tea on hyperglycemia and vascular complications of diabetes, we report the extraction and composition as well as the vasculoprotective effects of black tea extract (BTE), green tea extract (GTE), and dark tea extract (DTE).
30549892	2	29	theme	BTE	522:524	arg1	polysaccharides					503:517	polysaccharides	503:517	polysaccharides	503:517	High Performance Liquid Chromatography (HPLC) and colorimetric methods were conducted to analyze for tea catechins, caffeine, polyphenols, amino acids and polysaccharides of BTE, GTE and DTE.
30549892	2	29	theme	BTE	522:524	arg1	acids					493:497	amino acids	487:497	amino acids	487:497	High Performance Liquid Chromatography (HPLC) and colorimetric methods were conducted to analyze for tea catechins, caffeine, polyphenols, amino acids and polysaccharides of BTE, GTE and DTE.
30549892	2	29	theme	BTE	522:524	arg1	catechins					453:461	tea catechins	449:461	tea catechins	449:461	High Performance Liquid Chromatography (HPLC) and colorimetric methods were conducted to analyze for tea catechins, caffeine, polyphenols, amino acids and polysaccharides of BTE, GTE and DTE.
30549892	3	30	theme	α-glucosidase	566:578	arg1	effects					555:561	The inhibitory effects	540:561	The inhibitory effects of α-glucosidase, aldose reductase (AR), advanced glycation end-products (AGEs) and glucose uptake promotion effect in BTE, GTE and DTE	540:697	The inhibitory effects of α-glucosidase, aldose reductase (AR), advanced glycation end-products (AGEs) and glucose uptake promotion effect in BTE, GTE and DTE were explored in vitro.
30549892	5	31	theme	a-glucosidase	889:901	arg1	effects					878:884	the inhibitory effects	863:884	the inhibitory effects of a-glucosidase, AR, and AGEs	863:915	BTE, GTE, and DTE showed the inhibitory effects of a-glucosidase, AR, and AGEs, but only DTE exhibited the glucose uptake promotion effect in HepG2 cells.
30549892	0	32	theme	Water	29:33	arg1	Extracts					35:42	Water Extracts	29:42	Water Extracts of Black, Green and Dark Tea	29:71	Vasculoprotective Effects of Water Extracts of Black, Green and Dark Tea in Vitro.
30549892	1	33	theme	diabetes	175:182	arg1	complications					158:170	vascular complications	149:170	vascular complications of diabetes	149:182	To investigate the preventive effects of tea on hyperglycemia and vascular complications of diabetes, we report the extraction and composition as well as the vasculoprotective effects of black tea extract (BTE), green tea extract (GTE), and dark tea extract (DTE).
30549892	1	33	theme	diabetes	175:182	arg1	hyperglycemia					131:143	hyperglycemia	131:143	hyperglycemia	131:143	To investigate the preventive effects of tea on hyperglycemia and vascular complications of diabetes, we report the extraction and composition as well as the vasculoprotective effects of black tea extract (BTE), green tea extract (GTE), and dark tea extract (DTE).
30549892	3	34	theme	end-products	623:634	arg1	effects					555:561	The inhibitory effects	540:561	The inhibitory effects of α-glucosidase, aldose reductase (AR), advanced glycation end-products (AGEs) and glucose uptake promotion effect in BTE, GTE and DTE	540:697	The inhibitory effects of α-glucosidase, aldose reductase (AR), advanced glycation end-products (AGEs) and glucose uptake promotion effect in BTE, GTE and DTE were explored in vitro.
30549892	4	35	theme	catechin	770:777	arg1	Contents					723:730	Contents	723:730	Contents of six major catechin forms and total catechin as well as polyphenols	723:800	Contents of six major catechin forms and total catechin as well as polyphenols are higher in GTE and DTE than BTE.
30549892	5	36	theme	AGEs	912:915	arg1	effects					878:884	the inhibitory effects	863:884	the inhibitory effects of a-glucosidase, AR, and AGEs	863:915	BTE, GTE, and DTE showed the inhibitory effects of a-glucosidase, AR, and AGEs, but only DTE exhibited the glucose uptake promotion effect in HepG2 cells.
30549892	1	37	theme	black	270:274	arg1	tea					276:278	black tea extract (BTE), green tea extract (GTE), and dark tea extract (DTE)	270:345	tea	276:278	To investigate the preventive effects of tea on hyperglycemia and vascular complications of diabetes, we report the extraction and composition as well as the vasculoprotective effects of black tea extract (BTE), green tea extract (GTE), and dark tea extract (DTE).
30549892	6	38	theme	diabetes	1129:1136	arg1	progression					1066:1076	the progression	1062:1076	the progression of hyperglycemia	1062:1093	The results suggest that regular consumption of tea can help prevent the progression of hyperglycemia and the vascular complications of diabetes.
30549892	6	38	theme	diabetes	1129:1136	arg1	complications					1112:1124	the vascular complications	1099:1124	the vascular complications of diabetes	1099:1136	The results suggest that regular consumption of tea can help prevent the progression of hyperglycemia and the vascular complications of diabetes.
30549892	3	39	theme	inhibitory	544:553	arg1	effects					555:561	The inhibitory effects	540:561	The inhibitory effects of α-glucosidase, aldose reductase (AR), advanced glycation end-products (AGEs) and glucose uptake promotion effect in BTE, GTE and DTE	540:697	The inhibitory effects of α-glucosidase, aldose reductase (AR), advanced glycation end-products (AGEs) and glucose uptake promotion effect in BTE, GTE and DTE were explored in vitro.
30549892	0	40	theme	Extracts	35:42	arg1	Effects					18:24	Vasculoprotective Effects	0:24	Vasculoprotective Effects of Water Extracts of Black, Green and Dark Tea in Vitro	0:80	Vasculoprotective Effects of Water Extracts of Black, Green and Dark Tea in Vitro.
30549892	1	41	theme	tea	276:278	arg1	composition					214:224	composition	214:224	composition	214:224	To investigate the preventive effects of tea on hyperglycemia and vascular complications of diabetes, we report the extraction and composition as well as the vasculoprotective effects of black tea extract (BTE), green tea extract (GTE), and dark tea extract (DTE).
30549892	1	41	theme	tea	276:278	arg1	extraction					199:208	extraction	199:208	extraction	199:208	To investigate the preventive effects of tea on hyperglycemia and vascular complications of diabetes, we report the extraction and composition as well as the vasculoprotective effects of black tea extract (BTE), green tea extract (GTE), and dark tea extract (DTE).
30549892	1	41	theme	tea	276:278	arg1	effects					259:265	the vasculoprotective effects	237:265	the extraction and composition as well as the vasculoprotective effects of black tea extract (BTE), green tea extract (GTE), and dark tea extract (DTE)	195:345	To investigate the preventive effects of tea on hyperglycemia and vascular complications of diabetes, we report the extraction and composition as well as the vasculoprotective effects of black tea extract (BTE), green tea extract (GTE), and dark tea extract (DTE).
30549892	2	42	theme	tea	449:451	arg1	polysaccharides					503:517	polysaccharides	503:517	polysaccharides	503:517	High Performance Liquid Chromatography (HPLC) and colorimetric methods were conducted to analyze for tea catechins, caffeine, polyphenols, amino acids and polysaccharides of BTE, GTE and DTE.
30549892	2	42	theme	tea	449:451	arg1	polyphenols					474:484	polyphenols	474:484	polyphenols	474:484	High Performance Liquid Chromatography (HPLC) and colorimetric methods were conducted to analyze for tea catechins, caffeine, polyphenols, amino acids and polysaccharides of BTE, GTE and DTE.
30549892	2	42	theme	tea	449:451	arg1	acids					493:497	amino acids	487:497	amino acids	487:497	High Performance Liquid Chromatography (HPLC) and colorimetric methods were conducted to analyze for tea catechins, caffeine, polyphenols, amino acids and polysaccharides of BTE, GTE and DTE.
30549892	2	42	theme	tea	449:451	arg1	caffeine					464:471	caffeine	464:471	caffeine	464:471	High Performance Liquid Chromatography (HPLC) and colorimetric methods were conducted to analyze for tea catechins, caffeine, polyphenols, amino acids and polysaccharides of BTE, GTE and DTE.
30549892	2	42	theme	tea	449:451	arg1	catechins					453:461	tea catechins	449:461	tea catechins	449:461	High Performance Liquid Chromatography (HPLC) and colorimetric methods were conducted to analyze for tea catechins, caffeine, polyphenols, amino acids and polysaccharides of BTE, GTE and DTE.
30549892	1	43	theme	dark	324:327	arg1	DTE					342:344	DTE	342:344	DTE	342:344	To investigate the preventive effects of tea on hyperglycemia and vascular complications of diabetes, we report the extraction and composition as well as the vasculoprotective effects of black tea extract (BTE), green tea extract (GTE), and dark tea extract (DTE).
30549892	1	43	theme	dark	324:327	arg1	extract					333:339	dark tea extract	324:339	dark tea extract (DTE)	324:345	To investigate the preventive effects of tea on hyperglycemia and vascular complications of diabetes, we report the extraction and composition as well as the vasculoprotective effects of black tea extract (BTE), green tea extract (GTE), and dark tea extract (DTE).
30549892	2	44	theme	High	348:351	arg1	HPLC					388:391	HPLC	388:391	HPLC	388:391	High Performance Liquid Chromatography (HPLC) and colorimetric methods were conducted to analyze for tea catechins, caffeine, polyphenols, amino acids and polysaccharides of BTE, GTE and DTE.
30549892	2	44	theme	High	348:351	arg1	Chromatography					372:385	High Performance Liquid Chromatography	348:385	High Performance Liquid Chromatography (HPLC)	348:392	High Performance Liquid Chromatography (HPLC) and colorimetric methods were conducted to analyze for tea catechins, caffeine, polyphenols, amino acids and polysaccharides of BTE, GTE and DTE.
30549892	5	45	theme	promotion	960:968	arg1	effect					970:975	the glucose uptake promotion effect	941:975	the glucose uptake promotion effect in HepG2 cells	941:990	BTE, GTE, and DTE showed the inhibitory effects of a-glucosidase, AR, and AGEs, but only DTE exhibited the glucose uptake promotion effect in HepG2 cells.
30549892	3	46	theme	promotion	662:670	arg1	effect					672:677	promotion effect	662:677	promotion effect	662:677	The inhibitory effects of α-glucosidase, aldose reductase (AR), advanced glycation end-products (AGEs) and glucose uptake promotion effect in BTE, GTE and DTE were explored in vitro.
30549892	2	47	theme	amino	487:491	arg1	acids					493:497	amino acids	487:497	amino acids	487:497	High Performance Liquid Chromatography (HPLC) and colorimetric methods were conducted to analyze for tea catechins, caffeine, polyphenols, amino acids and polysaccharides of BTE, GTE and DTE.
30549892	2	47	theme	amino	487:491	arg1	catechins					453:461	tea catechins	449:461	tea catechins	449:461	High Performance Liquid Chromatography (HPLC) and colorimetric methods were conducted to analyze for tea catechins, caffeine, polyphenols, amino acids and polysaccharides of BTE, GTE and DTE.
30549892	1	48	dep	extraction	199:208	arg1	the					195:197	the	195:197	the	195:197	To investigate the preventive effects of tea on hyperglycemia and vascular complications of diabetes, we report the extraction and composition as well as the vasculoprotective effects of black tea extract (BTE), green tea extract (GTE), and dark tea extract (DTE).
30549892	6	49	theme	regular	1018:1024	arg1	consumption					1026:1036	regular consumption	1018:1036	regular consumption of tea	1018:1043	The results suggest that regular consumption of tea can help prevent the progression of hyperglycemia and the vascular complications of diabetes.
30549892	4	50	theme	total	764:768	arg1	catechin					770:777	total catechin	764:777	total catechin	764:777	Contents of six major catechin forms and total catechin as well as polyphenols are higher in GTE and DTE than BTE.
30549892	0	51	theme	Black	47:51	arg1	Tea					69:71	Black, Green and Dark Tea	47:71	Tea	69:71	Vasculoprotective Effects of Water Extracts of Black, Green and Dark Tea in Vitro.
30549892	4	52	theme	forms	754:758	arg1	Contents					723:730	Contents	723:730	Contents of six major catechin forms and total catechin as well as polyphenols	723:800	Contents of six major catechin forms and total catechin as well as polyphenols are higher in GTE and DTE than BTE.
30549892	4	53	theme	catechin	745:752	arg1	forms					754:758	six major catechin forms	735:758	six major catechin forms	735:758	Contents of six major catechin forms and total catechin as well as polyphenols are higher in GTE and DTE than BTE.
30549892	1	54	theme	extract	333:339	arg1	composition					214:224	composition	214:224	composition	214:224	To investigate the preventive effects of tea on hyperglycemia and vascular complications of diabetes, we report the extraction and composition as well as the vasculoprotective effects of black tea extract (BTE), green tea extract (GTE), and dark tea extract (DTE).
30549892	1	54	theme	extract	333:339	arg1	extraction					199:208	extraction	199:208	extraction	199:208	To investigate the preventive effects of tea on hyperglycemia and vascular complications of diabetes, we report the extraction and composition as well as the vasculoprotective effects of black tea extract (BTE), green tea extract (GTE), and dark tea extract (DTE).
30549892	1	54	theme	extract	333:339	arg1	effects					259:265	the vasculoprotective effects	237:265	the extraction and composition as well as the vasculoprotective effects of black tea extract (BTE), green tea extract (GTE), and dark tea extract (DTE)	195:345	To investigate the preventive effects of tea on hyperglycemia and vascular complications of diabetes, we report the extraction and composition as well as the vasculoprotective effects of black tea extract (BTE), green tea extract (GTE), and dark tea extract (DTE).
30549892	3	55	theme	glucose	647:653	arg1	uptake					655:660	glucose uptake	647:660	glucose uptake	647:660	The inhibitory effects of α-glucosidase, aldose reductase (AR), advanced glycation end-products (AGEs) and glucose uptake promotion effect in BTE, GTE and DTE were explored in vitro.
30549892	3	56	theme	aldose	581:586	arg1	AR					599:600	AR	599:600	AR	599:600	The inhibitory effects of α-glucosidase, aldose reductase (AR), advanced glycation end-products (AGEs) and glucose uptake promotion effect in BTE, GTE and DTE were explored in vitro.
30549892	3	56	theme	aldose	581:586	arg1	reductase					588:596	aldose reductase	581:596	aldose reductase (AR)	581:601	The inhibitory effects of α-glucosidase, aldose reductase (AR), advanced glycation end-products (AGEs) and glucose uptake promotion effect in BTE, GTE and DTE were explored in vitro.
30549892	1	57	theme	preventive	102:111	arg1	effects					113:119	the preventive effects	98:119	the preventive effects of tea on hyperglycemia and vascular complications of diabetes	98:182	To investigate the preventive effects of tea on hyperglycemia and vascular complications of diabetes, we report the extraction and composition as well as the vasculoprotective effects of black tea extract (BTE), green tea extract (GTE), and dark tea extract (DTE).
30549892	5	58	theme	inhibitory	867:876	arg1	effects					878:884	the inhibitory effects	863:884	the inhibitory effects of a-glucosidase, AR, and AGEs	863:915	BTE, GTE, and DTE showed the inhibitory effects of a-glucosidase, AR, and AGEs, but only DTE exhibited the glucose uptake promotion effect in HepG2 cells.
30549892	3	59	from	effects	555:561	arg1	BTE					682:684	BTE	682:684	BTE	682:684	The inhibitory effects of α-glucosidase, aldose reductase (AR), advanced glycation end-products (AGEs) and glucose uptake promotion effect in BTE, GTE and DTE were explored in vitro.
30549892	3	59	from	effects	555:561	arg1	DTE					695:697	DTE	695:697	DTE	695:697	The inhibitory effects of α-glucosidase, aldose reductase (AR), advanced glycation end-products (AGEs) and glucose uptake promotion effect in BTE, GTE and DTE were explored in vitro.
30549892	3	59	from	effects	555:561	arg1	GTE					687:689	GTE	687:689	GTE	687:689	The inhibitory effects of α-glucosidase, aldose reductase (AR), advanced glycation end-products (AGEs) and glucose uptake promotion effect in BTE, GTE and DTE were explored in vitro.
30549892	5	60	theme	only	922:925	arg1	DTE					927:929	only DTE	922:929	only DTE	922:929	BTE, GTE, and DTE showed the inhibitory effects of a-glucosidase, AR, and AGEs, but only DTE exhibited the glucose uptake promotion effect in HepG2 cells.
30549892	0	61	theme	Green	54:58	arg1	Tea					69:71	Black, Green and Dark Tea	47:71	Tea	69:71	Vasculoprotective Effects of Water Extracts of Black, Green and Dark Tea in Vitro.
30549892	6	62	theme	tea	1041:1043	arg1	consumption					1026:1036	regular consumption	1018:1036	regular consumption of tea	1018:1043	The results suggest that regular consumption of tea can help prevent the progression of hyperglycemia and the vascular complications of diabetes.
30549892	1	63	theme	tea	124:126	arg1	effects					113:119	the preventive effects	98:119	the preventive effects of tea on hyperglycemia and vascular complications of diabetes	98:182	To investigate the preventive effects of tea on hyperglycemia and vascular complications of diabetes, we report the extraction and composition as well as the vasculoprotective effects of black tea extract (BTE), green tea extract (GTE), and dark tea extract (DTE).
30549892	5	64	theme	glucose	945:951	arg1	effect					970:975	the glucose uptake promotion effect	941:975	the glucose uptake promotion effect in HepG2 cells	941:990	BTE, GTE, and DTE showed the inhibitory effects of a-glucosidase, AR, and AGEs, but only DTE exhibited the glucose uptake promotion effect in HepG2 cells.
28117421	0	0	theme	Properties	76:85	arg1	Tuning					57:62	Tuning	57:62	Tuning	57:62	Advanced Materials From Fungal Mycelium: Fabrication and Tuning of Physical Properties.
28117421	0	0	theme	Properties	76:85	arg1	Fabrication					41:51	Fabrication	41:51	Fabrication	41:51	Advanced Materials From Fungal Mycelium: Fabrication and Tuning of Physical Properties.
28117421	0	0	theme	Properties	76:85	arg1	Materials					9:17	Advanced Materials	0:17	Advanced Materials From Fungal Mycelium: Fabrication and Tuning of Physical Properties.	0:86	Advanced Materials From Fungal Mycelium: Fabrication and Tuning of Physical Properties.
28117421	8	1	theme	scale	1466:1470	arg1	applications					1472:1483	various scale applications	1458:1483	various scale applications	1458:1483	The possibility of tailoring mycelium materials' properties by properly choosing their nutrient substrates paves the way for their use in various scale applications.
28117421	1	2	theme	self-growing	132:143	arg1	materials					173:181	self-growing, fibrous, natural composite materials	132:181	self-growing, fibrous, natural composite materials	132:181	In this work is presented a new category of self-growing, fibrous, natural composite materials with controlled physical properties that can be produced in large quantities and over wide areas, based on mycelium, the main body of fungi.
28117421	0	3	theme	Physical	67:74	arg1	Properties					76:85	Physical Properties	67:85	Physical Properties	67:85	Advanced Materials From Fungal Mycelium: Fabrication and Tuning of Physical Properties.
28117421	1	4	theme	main	304:307	arg1	body					309:312	the main body	300:312	the main body of fungi	300:321	In this work is presented a new category of self-growing, fibrous, natural composite materials with controlled physical properties that can be produced in large quantities and over wide areas, based on mycelium, the main body of fungi.
28117421	4	5	theme	relative	800:807	arg1	concentrations					809:822	different relative concentrations	790:822	different relative concentrations in polysaccharides, lipids, proteins and chitin	790:870	Depending on their feeding substrate, the final fibrous structures showed different relative concentrations in polysaccharides, lipids, proteins and chitin.
28117421	2	6	theme	sugars	596:601	arg1	presence					577:584	presence	577:584	presence of simple sugars in its composition	577:620	Mycelia from two types of edible, medicinal fungi, Ganoderma lucidum and Pleurotus ostreatus, have been carefully cultivated, being fed by two bio-substrates: cellulose and cellulose/potato-dextrose, the second being easier to digest by mycelium due to presence of simple sugars in its composition.
28117421	2	7	from	composition	610:620	arg1	presence					577:584	presence	577:584	presence of simple sugars in its composition	577:620	Mycelia from two types of edible, medicinal fungi, Ganoderma lucidum and Pleurotus ostreatus, have been carefully cultivated, being fed by two bio-substrates: cellulose and cellulose/potato-dextrose, the second being easier to digest by mycelium due to presence of simple sugars in its composition.
28117421	5	8	from	alterations	907:917	arg1	morphology					922:931	morphology	922:931	morphology	922:931	Such differences are reflected as alterations in morphology and mechanical properties.
28117421	5	8	from	alterations	907:917	arg1	properties					948:957	mechanical properties	937:957	mechanical properties	937:957	Such differences are reflected as alterations in morphology and mechanical properties.
28117421	3	9	theme	specific	629:636	arg1	times					646:650	specific growing times	629:650	specific growing times	629:650	After specific growing times the mycelia have been processed in order to cease their growth.
28117421	1	10	with	category	120:127	arg1	properties					208:217	controlled physical properties	188:217	controlled physical properties that can be produced in large quantities	188:258	In this work is presented a new category of self-growing, fibrous, natural composite materials with controlled physical properties that can be produced in large quantities and over wide areas, based on mycelium, the main body of fungi.
28117421	2	11	theme	simple	589:594	arg1	sugars					596:601	simple sugars	589:601	simple sugars in its composition	589:620	Mycelia from two types of edible, medicinal fungi, Ganoderma lucidum and Pleurotus ostreatus, have been carefully cultivated, being fed by two bio-substrates: cellulose and cellulose/potato-dextrose, the second being easier to digest by mycelium due to presence of simple sugars in its composition.
28117421	8	12	theme	mycelium	1349:1356	arg1	properties					1369:1378	mycelium materials' properties	1349:1378	mycelium materials' properties	1349:1378	The possibility of tailoring mycelium materials' properties by properly choosing their nutrient substrates paves the way for their use in various scale applications.
28117421	1	13	theme	large	243:247	arg1	quantities					249:258	large quantities	243:258	large quantities	243:258	In this work is presented a new category of self-growing, fibrous, natural composite materials with controlled physical properties that can be produced in large quantities and over wide areas, based on mycelium, the main body of fungi.
28117421	2	14	dep	bio-substrates	467:480	arg1	cellulose					483:491	cellulose	483:491	cellulose	483:491	Mycelia from two types of edible, medicinal fungi, Ganoderma lucidum and Pleurotus ostreatus, have been carefully cultivated, being fed by two bio-substrates: cellulose and cellulose/potato-dextrose, the second being easier to digest by mycelium due to presence of simple sugars in its composition.
28117421	2	14	dep	bio-substrates	467:480	arg1	bio-substrates					467:480	two bio-substrates	463:480	two bio-substrates: cellulose and cellulose/potato-dextrose	463:521	Mycelia from two types of edible, medicinal fungi, Ganoderma lucidum and Pleurotus ostreatus, have been carefully cultivated, being fed by two bio-substrates: cellulose and cellulose/potato-dextrose, the second being easier to digest by mycelium due to presence of simple sugars in its composition.
28117421	2	14	dep	bio-substrates	467:480	arg1	cellulose/potato-dextrose					497:521	cellulose/potato-dextrose	497:521	cellulose/potato-dextrose	497:521	Mycelia from two types of edible, medicinal fungi, Ganoderma lucidum and Pleurotus ostreatus, have been carefully cultivated, being fed by two bio-substrates: cellulose and cellulose/potato-dextrose, the second being easier to digest by mycelium due to presence of simple sugars in its composition.
28117421	6	15	contain	contained	993:1001	arg2	higher					1026:1031	higher	1026:1031	higher	1026:1031	The materials grown on cellulose contained more chitin and showed higher Young's modulus and lower elongation than those grown on dextrose-containing substrates, indicating that the mycelium materials get stiffer when their feeding substrate is harder to digest.
28117421	6	15	contain	contained	993:1001	arg1	materials					964:972	The materials	960:972	The materials grown on cellulose	960:991	The materials grown on cellulose contained more chitin and showed higher Young's modulus and lower elongation than those grown on dextrose-containing substrates, indicating that the mycelium materials get stiffer when their feeding substrate is harder to digest.
28117421	6	15	contain	contained	993:1001	arg2	lower					1053:1057	lower	1053:1057	lower	1053:1057	The materials grown on cellulose contained more chitin and showed higher Young's modulus and lower elongation than those grown on dextrose-containing substrates, indicating that the mycelium materials get stiffer when their feeding substrate is harder to digest.
28117421	6	16	theme	mycelium	1142:1149	arg1	materials					1151:1159	the mycelium materials	1138:1159	the mycelium materials	1138:1159	The materials grown on cellulose contained more chitin and showed higher Young's modulus and lower elongation than those grown on dextrose-containing substrates, indicating that the mycelium materials get stiffer when their feeding substrate is harder to digest.
28117421	5	17	theme	Such	873:876	arg1	differences					878:888	Such differences	873:888	Such differences	873:888	Such differences are reflected as alterations in morphology and mechanical properties.
28117421	5	17	theme	Such	873:876	arg1	alterations					907:917	alterations	907:917	alterations in morphology and mechanical properties	907:957	Such differences are reflected as alterations in morphology and mechanical properties.
28117421	8	18	from	use	1451:1453	arg1	applications					1472:1483	various scale applications	1458:1483	various scale applications	1458:1483	The possibility of tailoring mycelium materials' properties by properly choosing their nutrient substrates paves the way for their use in various scale applications.
28117421	1	19	theme	fungi	317:321	arg1	body					309:312	the main body	300:312	the main body of fungi	300:321	In this work is presented a new category of self-growing, fibrous, natural composite materials with controlled physical properties that can be produced in large quantities and over wide areas, based on mycelium, the main body of fungi.
28117421	7	20	theme	higher	1302:1307	arg1	angles					1295:1300	water contact angles	1281:1300	water contact angles higher than 120°	1281:1317	All the developed fibrous materials were hydrophobic with water contact angles higher than 120°.
28117421	4	21	theme	fibrous	764:770	arg1	structures					772:781	the final fibrous structures	754:781	the final fibrous structures	754:781	Depending on their feeding substrate, the final fibrous structures showed different relative concentrations in polysaccharides, lipids, proteins and chitin.
28117421	6	22	theme	Young	1033:1037	arg1	modulus					1041:1047	Young's modulus	1033:1047	Young's modulus	1033:1047	The materials grown on cellulose contained more chitin and showed higher Young's modulus and lower elongation than those grown on dextrose-containing substrates, indicating that the mycelium materials get stiffer when their feeding substrate is harder to digest.
28117421	0	23	from	Mycelium	31:38	arg1	Tuning					57:62	Tuning	57:62	Tuning	57:62	Advanced Materials From Fungal Mycelium: Fabrication and Tuning of Physical Properties.
28117421	0	23	from	Mycelium	31:38	arg1	Fabrication					41:51	Fabrication	41:51	Fabrication	41:51	Advanced Materials From Fungal Mycelium: Fabrication and Tuning of Physical Properties.
28117421	0	23	from	Mycelium	31:38	arg1	Materials					9:17	Advanced Materials	0:17	Advanced Materials From Fungal Mycelium: Fabrication and Tuning of Physical Properties.	0:86	Advanced Materials From Fungal Mycelium: Fabrication and Tuning of Physical Properties.
28117421	4	24	theme	different	790:798	arg1	concentrations					809:822	different relative concentrations	790:822	different relative concentrations in polysaccharides, lipids, proteins and chitin	790:870	Depending on their feeding substrate, the final fibrous structures showed different relative concentrations in polysaccharides, lipids, proteins and chitin.
28117421	1	25	dep	self-growing	132:143	arg1	natural					155:161	natural	155:161	natural	155:161	In this work is presented a new category of self-growing, fibrous, natural composite materials with controlled physical properties that can be produced in large quantities and over wide areas, based on mycelium, the main body of fungi.
28117421	1	25	dep	self-growing	132:143	arg1	fibrous					146:152	fibrous	146:152	fibrous	146:152	In this work is presented a new category of self-growing, fibrous, natural composite materials with controlled physical properties that can be produced in large quantities and over wide areas, based on mycelium, the main body of fungi.
28117421	7	26	theme	developed	1231:1239	arg1	hydrophobic					1264:1274	hydrophobic	1264:1274	hydrophobic	1264:1274	All the developed fibrous materials were hydrophobic with water contact angles higher than 120°.
28117421	7	26	theme	developed	1231:1239	arg1	materials					1249:1257	All the developed fibrous materials	1223:1257	All the developed fibrous materials	1223:1257	All the developed fibrous materials were hydrophobic with water contact angles higher than 120°.
28117421	7	27	theme	contact	1287:1293	arg1	angles					1295:1300	water contact angles	1281:1300	water contact angles higher than 120°	1281:1317	All the developed fibrous materials were hydrophobic with water contact angles higher than 120°.
28117421	0	28	theme	Advanced	0:7	arg1	Tuning					57:62	Tuning	57:62	Tuning	57:62	Advanced Materials From Fungal Mycelium: Fabrication and Tuning of Physical Properties.
28117421	0	28	theme	Advanced	0:7	arg1	Fabrication					41:51	Fabrication	41:51	Fabrication	41:51	Advanced Materials From Fungal Mycelium: Fabrication and Tuning of Physical Properties.
28117421	0	28	theme	Advanced	0:7	arg1	Materials					9:17	Advanced Materials	0:17	Advanced Materials From Fungal Mycelium: Fabrication and Tuning of Physical Properties.	0:86	Advanced Materials From Fungal Mycelium: Fabrication and Tuning of Physical Properties.
28117421	2	29	dep	edible	350:355	arg1	medicinal					358:366	medicinal	358:366	medicinal	358:366	Mycelia from two types of edible, medicinal fungi, Ganoderma lucidum and Pleurotus ostreatus, have been carefully cultivated, being fed by two bio-substrates: cellulose and cellulose/potato-dextrose, the second being easier to digest by mycelium due to presence of simple sugars in its composition.
28117421	6	30	dep	higher	1026:1031	arg1	modulus					1041:1047	Young's modulus	1033:1047	Young's modulus	1033:1047	The materials grown on cellulose contained more chitin and showed higher Young's modulus and lower elongation than those grown on dextrose-containing substrates, indicating that the mycelium materials get stiffer when their feeding substrate is harder to digest.
28117421	6	30	dep	higher	1026:1031	arg1	elongation					1059:1068	elongation	1059:1068	elongation	1059:1068	The materials grown on cellulose contained more chitin and showed higher Young's modulus and lower elongation than those grown on dextrose-containing substrates, indicating that the mycelium materials get stiffer when their feeding substrate is harder to digest.
28117421	1	31	theme	composite	163:171	arg1	materials					173:181	self-growing, fibrous, natural composite materials	132:181	self-growing, fibrous, natural composite materials	132:181	In this work is presented a new category of self-growing, fibrous, natural composite materials with controlled physical properties that can be produced in large quantities and over wide areas, based on mycelium, the main body of fungi.
28117421	0	32	theme	Fungal	24:29	arg1	Mycelium					31:38	Fungal Mycelium	24:38	Fungal Mycelium	24:38	Advanced Materials From Fungal Mycelium: Fabrication and Tuning of Physical Properties.
28117421	4	33	theme	feeding	735:741	arg1	substrate					743:751	their feeding substrate	729:751	their feeding substrate	729:751	Depending on their feeding substrate, the final fibrous structures showed different relative concentrations in polysaccharides, lipids, proteins and chitin.
28117421	2	34	theme	fungi	368:372	arg1	types					341:345	two types	337:345	two types of edible, medicinal fungi	337:372	Mycelia from two types of edible, medicinal fungi, Ganoderma lucidum and Pleurotus ostreatus, have been carefully cultivated, being fed by two bio-substrates: cellulose and cellulose/potato-dextrose, the second being easier to digest by mycelium due to presence of simple sugars in its composition.
28117421	2	34	theme	fungi	368:372	arg1	lucidum					385:391	Ganoderma lucidum	375:391	Ganoderma lucidum	375:391	Mycelia from two types of edible, medicinal fungi, Ganoderma lucidum and Pleurotus ostreatus, have been carefully cultivated, being fed by two bio-substrates: cellulose and cellulose/potato-dextrose, the second being easier to digest by mycelium due to presence of simple sugars in its composition.
28117421	2	34	theme	fungi	368:372	arg1	ostreatus					407:415	Pleurotus ostreatus	397:415	Pleurotus ostreatus	397:415	Mycelia from two types of edible, medicinal fungi, Ganoderma lucidum and Pleurotus ostreatus, have been carefully cultivated, being fed by two bio-substrates: cellulose and cellulose/potato-dextrose, the second being easier to digest by mycelium due to presence of simple sugars in its composition.
28117421	1	35	theme	wide	269:272	arg1	areas					274:278	wide areas	269:278	wide areas	269:278	In this work is presented a new category of self-growing, fibrous, natural composite materials with controlled physical properties that can be produced in large quantities and over wide areas, based on mycelium, the main body of fungi.
28117421	7	36	with	hydrophobic	1264:1274	arg1	angles					1295:1300	water contact angles	1281:1300	water contact angles higher than 120°	1281:1317	All the developed fibrous materials were hydrophobic with water contact angles higher than 120°.
28117421	6	37	theme	dextrose-containing	1090:1108	arg1	substrates					1110:1119	dextrose-containing substrates	1090:1119	dextrose-containing substrates	1090:1119	The materials grown on cellulose contained more chitin and showed higher Young's modulus and lower elongation than those grown on dextrose-containing substrates, indicating that the mycelium materials get stiffer when their feeding substrate is harder to digest.
28117421	7	38	theme	water	1281:1285	arg1	angles					1295:1300	water contact angles	1281:1300	water contact angles higher than 120°	1281:1317	All the developed fibrous materials were hydrophobic with water contact angles higher than 120°.
28117421	2	39	dep	easier	541:546	arg1	to					548:549	to	548:549	to	548:549	Mycelia from two types of edible, medicinal fungi, Ganoderma lucidum and Pleurotus ostreatus, have been carefully cultivated, being fed by two bio-substrates: cellulose and cellulose/potato-dextrose, the second being easier to digest by mycelium due to presence of simple sugars in its composition.
28117421	2	39	dep	easier	541:546	arg1	digest					551:556	digest	551:556	digest	551:556	Mycelia from two types of edible, medicinal fungi, Ganoderma lucidum and Pleurotus ostreatus, have been carefully cultivated, being fed by two bio-substrates: cellulose and cellulose/potato-dextrose, the second being easier to digest by mycelium due to presence of simple sugars in its composition.
28117421	1	40	theme	controlled	188:197	arg1	properties					208:217	controlled physical properties	188:217	controlled physical properties that can be produced in large quantities	188:258	In this work is presented a new category of self-growing, fibrous, natural composite materials with controlled physical properties that can be produced in large quantities and over wide areas, based on mycelium, the main body of fungi.
28117421	2	41	theme	Ganoderma	375:383	arg1	lucidum					385:391	Ganoderma lucidum	375:391	Ganoderma lucidum	375:391	Mycelia from two types of edible, medicinal fungi, Ganoderma lucidum and Pleurotus ostreatus, have been carefully cultivated, being fed by two bio-substrates: cellulose and cellulose/potato-dextrose, the second being easier to digest by mycelium due to presence of simple sugars in its composition.
28117421	2	41	theme	Ganoderma	375:383	arg1	types					341:345	two types	337:345	two types of edible, medicinal fungi	337:372	Mycelia from two types of edible, medicinal fungi, Ganoderma lucidum and Pleurotus ostreatus, have been carefully cultivated, being fed by two bio-substrates: cellulose and cellulose/potato-dextrose, the second being easier to digest by mycelium due to presence of simple sugars in its composition.
28117421	1	42	theme	physical	199:206	arg1	properties					208:217	controlled physical properties	188:217	controlled physical properties that can be produced in large quantities	188:258	In this work is presented a new category of self-growing, fibrous, natural composite materials with controlled physical properties that can be produced in large quantities and over wide areas, based on mycelium, the main body of fungi.
28117421	4	43	theme	final	758:762	arg1	structures					772:781	the final fibrous structures	754:781	the final fibrous structures	754:781	Depending on their feeding substrate, the final fibrous structures showed different relative concentrations in polysaccharides, lipids, proteins and chitin.
28117421	4	44	from	concentrations	809:822	arg1	chitin					865:870	chitin	865:870	chitin	865:870	Depending on their feeding substrate, the final fibrous structures showed different relative concentrations in polysaccharides, lipids, proteins and chitin.
28117421	4	44	from	concentrations	809:822	arg1	proteins					852:859	proteins	852:859	proteins	852:859	Depending on their feeding substrate, the final fibrous structures showed different relative concentrations in polysaccharides, lipids, proteins and chitin.
28117421	4	44	from	concentrations	809:822	arg1	lipids					844:849	lipids	844:849	lipids	844:849	Depending on their feeding substrate, the final fibrous structures showed different relative concentrations in polysaccharides, lipids, proteins and chitin.
28117421	4	44	from	concentrations	809:822	arg1	polysaccharides					827:841	polysaccharides	827:841	polysaccharides	827:841	Depending on their feeding substrate, the final fibrous structures showed different relative concentrations in polysaccharides, lipids, proteins and chitin.
28117421	2	45	theme	Pleurotus	397:405	arg1	types					341:345	two types	337:345	two types of edible, medicinal fungi	337:372	Mycelia from two types of edible, medicinal fungi, Ganoderma lucidum and Pleurotus ostreatus, have been carefully cultivated, being fed by two bio-substrates: cellulose and cellulose/potato-dextrose, the second being easier to digest by mycelium due to presence of simple sugars in its composition.
28117421	2	45	theme	Pleurotus	397:405	arg1	ostreatus					407:415	Pleurotus ostreatus	397:415	Pleurotus ostreatus	397:415	Mycelia from two types of edible, medicinal fungi, Ganoderma lucidum and Pleurotus ostreatus, have been carefully cultivated, being fed by two bio-substrates: cellulose and cellulose/potato-dextrose, the second being easier to digest by mycelium due to presence of simple sugars in its composition.
28117421	6	46	theme	feeding	1184:1190	arg1	substrate					1192:1200	their feeding substrate	1178:1200	their feeding substrate	1178:1200	The materials grown on cellulose contained more chitin and showed higher Young's modulus and lower elongation than those grown on dextrose-containing substrates, indicating that the mycelium materials get stiffer when their feeding substrate is harder to digest.
28117421	7	47	theme	fibrous	1241:1247	arg1	hydrophobic					1264:1274	hydrophobic	1264:1274	hydrophobic	1264:1274	All the developed fibrous materials were hydrophobic with water contact angles higher than 120°.
28117421	7	47	theme	fibrous	1241:1247	arg1	materials					1249:1257	All the developed fibrous materials	1223:1257	All the developed fibrous materials	1223:1257	All the developed fibrous materials were hydrophobic with water contact angles higher than 120°.
28117421	2	48	from	presence	577:584	arg1	composition					610:620	its composition	606:620	its composition	606:620	Mycelia from two types of edible, medicinal fungi, Ganoderma lucidum and Pleurotus ostreatus, have been carefully cultivated, being fed by two bio-substrates: cellulose and cellulose/potato-dextrose, the second being easier to digest by mycelium due to presence of simple sugars in its composition.
28117421	5	49	theme	mechanical	937:946	arg1	properties					948:957	mechanical properties	937:957	mechanical properties	937:957	Such differences are reflected as alterations in morphology and mechanical properties.
28117421	2	50	from	types	341:345	arg1	Mycelia					324:330	Mycelia	324:330	Mycelia from two types of edible, medicinal fungi, Ganoderma lucidum and Pleurotus ostreatus,	324:416	Mycelia from two types of edible, medicinal fungi, Ganoderma lucidum and Pleurotus ostreatus, have been carefully cultivated, being fed by two bio-substrates: cellulose and cellulose/potato-dextrose, the second being easier to digest by mycelium due to presence of simple sugars in its composition.
28117421	2	51	from	sugars	596:601	arg1	composition					610:620	its composition	606:620	its composition	606:620	Mycelia from two types of edible, medicinal fungi, Ganoderma lucidum and Pleurotus ostreatus, have been carefully cultivated, being fed by two bio-substrates: cellulose and cellulose/potato-dextrose, the second being easier to digest by mycelium due to presence of simple sugars in its composition.
28117421	8	52	theme	various	1458:1464	arg1	applications					1472:1483	various scale applications	1458:1483	various scale applications	1458:1483	The possibility of tailoring mycelium materials' properties by properly choosing their nutrient substrates paves the way for their use in various scale applications.
28117421	1	53	theme	new	116:118	arg1	category					120:127	a new category	114:127	a new category of self-growing, fibrous, natural composite materials with controlled physical properties that can be produced in large quantities and over wide areas	114:278	In this work is presented a new category of self-growing, fibrous, natural composite materials with controlled physical properties that can be produced in large quantities and over wide areas, based on mycelium, the main body of fungi.
28117421	2	54	theme	edible	350:355	arg1	fungi					368:372	edible, medicinal fungi	350:372	edible, medicinal fungi	350:372	Mycelia from two types of edible, medicinal fungi, Ganoderma lucidum and Pleurotus ostreatus, have been carefully cultivated, being fed by two bio-substrates: cellulose and cellulose/potato-dextrose, the second being easier to digest by mycelium due to presence of simple sugars in its composition.
28117421	0	55	dep	Materials	9:17	arg1	Tuning					57:62	Tuning	57:62	Tuning	57:62	Advanced Materials From Fungal Mycelium: Fabrication and Tuning of Physical Properties.
28117421	0	55	dep	Materials	9:17	arg1	Fabrication					41:51	Fabrication	41:51	Fabrication	41:51	Advanced Materials From Fungal Mycelium: Fabrication and Tuning of Physical Properties.
28117421	0	55	dep	Materials	9:17	arg1	Materials					9:17	Advanced Materials	0:17	Advanced Materials From Fungal Mycelium: Fabrication and Tuning of Physical Properties.	0:86	Advanced Materials From Fungal Mycelium: Fabrication and Tuning of Physical Properties.
28117421	1	56	theme	materials	173:181	arg1	category					120:127	a new category	114:127	a new category of self-growing, fibrous, natural composite materials with controlled physical properties that can be produced in large quantities and over wide areas	114:278	In this work is presented a new category of self-growing, fibrous, natural composite materials with controlled physical properties that can be produced in large quantities and over wide areas, based on mycelium, the main body of fungi.
28117421	3	57	theme	growing	638:644	arg1	times					646:650	specific growing times	629:650	specific growing times	629:650	After specific growing times the mycelia have been processed in order to cease their growth.
28117421	8	58	theme	nutrient	1407:1414	arg1	substrates					1416:1425	their nutrient substrates	1401:1425	their nutrient substrates	1401:1425	The possibility of tailoring mycelium materials' properties by properly choosing their nutrient substrates paves the way for their use in various scale applications.
27998108	2	0	theme	[BMIm+Cl-	506:514	arg1	solution					517:524	the [BMIm+Cl-] solution	502:524	the [BMIm+Cl-] solution of [CEL+KER]	502:537	In this method, butylmethylimmidazolium chloride ([BMIm+Cl-]), a simple ionic liquid, was used as the sole solvent and silver chloride was added to the [BMIm+Cl-] solution of [CEL+KER] during the dissolution process.
27998108	1	1	theme	nanoparticles	320:332	arg1	form					286:289	the form	282:289	the form of either ionic (Ag+) or Ag0 nanoparticles (Ag+NPs or Ag0NPs)	282:351	A novel, simple method was developed to synthesize biocompatible composites containing 50% cellulose (CEL) and 50% keratin (KER) and silver in the form of either ionic (Ag+) or Ag0 nanoparticles (Ag+NPs or Ag0NPs).
27998108	3	2	from	silver	575:580	arg1	composites					589:598	the composites	585:598	the composites	585:598	The silver in the composites can be maintained as ionic silver (Ag+) or completely converted to metallic silver (Ag0) by reducing it with NaBH4.
27998108	8	3	theme	[CEL+KER+AgClNPs	1654:1669	arg1	composite					1672:1680	the [CEL+KER+AgClNPs] composite	1650:1680	the [CEL+KER+AgClNPs] composite	1650:1680	More importantly, for the same bacteria and the same silver content, the [CEL+KER+AgClNPs] composite is relatively more toxic than [CEL+KER+Ag0NPs] composite.
27998108	8	3	theme	[CEL+KER+AgClNPs	1654:1669	arg1	toxic					1701:1705	toxic	1701:1705	toxic	1701:1705	More importantly, for the same bacteria and the same silver content, the [CEL+KER+AgClNPs] composite is relatively more toxic than [CEL+KER+Ag0NPs] composite.
27998108	1	4	theme	%	252:252	arg1	KER					263:265	KER	263:265	KER	263:265	A novel, simple method was developed to synthesize biocompatible composites containing 50% cellulose (CEL) and 50% keratin (KER) and silver in the form of either ionic (Ag+) or Ag0 nanoparticles (Ag+NPs or Ag0NPs).
27998108	1	4	theme	%	252:252	arg1	keratin					254:260	50% keratin	250:260	50% keratin (KER)	250:266	A novel, simple method was developed to synthesize biocompatible composites containing 50% cellulose (CEL) and 50% keratin (KER) and silver in the form of either ionic (Ag+) or Ag0 nanoparticles (Ag+NPs or Ag0NPs).
27998108	4	5	theme	[Fourier	744:751	arg1	results					720:726	The results	716:726	The results of spectroscopy [Fourier transform infrared and X-ray diffraction (XRD)] and imaging [scanning electron microscopy (SEM)] measurements	716:861	The results of spectroscopy [Fourier transform infrared and X-ray diffraction (XRD)] and imaging [scanning electron microscopy (SEM)] measurements confirm that CEL and KER remain chemically intact and homogeneously distributed in the composites.
27998108	2	6	theme	[CEL+KER	529:536	arg1	solution					517:524	the [BMIm+Cl-] solution	502:524	the [BMIm+Cl-] solution of [CEL+KER]	502:537	In this method, butylmethylimmidazolium chloride ([BMIm+Cl-]), a simple ionic liquid, was used as the sole solvent and silver chloride was added to the [BMIm+Cl-] solution of [CEL+KER] during the dissolution process.
27998108	5	7	from	composites	1104:1113	arg1	form					1164:1167	either Ag+ (in the form of AgClNPs) or Ag0NPs form	1118:1167	either Ag+ (in the form of AgClNPs) or Ag0NPs form with sizes of 27 ± 2 or 9 ± 1 nm, respectively	1118:1214	Powder XRD and SEM results show that the silver in the [CEL+KER+Ag+] and [CEL+KER+Ag0] composites is homogeneously distributed throughout the composites in either Ag+ (in the form of AgClNPs) or Ag0NPs form with sizes of 27 ± 2 or 9 ± 1 nm, respectively.
27998108	5	8	theme	[CEL+KER+Ag0	1035:1046	arg1	composites					1049:1058	the [CEL+KER+Ag+] and [CEL+KER+Ag0] composites	1013:1058	composites	1049:1058	Powder XRD and SEM results show that the silver in the [CEL+KER+Ag+] and [CEL+KER+Ag0] composites is homogeneously distributed throughout the composites in either Ag+ (in the form of AgClNPs) or Ag0NPs form with sizes of 27 ± 2 or 9 ± 1 nm, respectively.
27998108	5	9	theme	nm	1199:1200	arg1	sizes					1174:1178	sizes	1174:1178	sizes of 27 ± 2 or 9 ± 1 nm, respectively	1174:1214	Powder XRD and SEM results show that the silver in the [CEL+KER+Ag+] and [CEL+KER+Ag0] composites is homogeneously distributed throughout the composites in either Ag+ (in the form of AgClNPs) or Ag0NPs form with sizes of 27 ± 2 or 9 ± 1 nm, respectively.
27998108	12	10	theme	chronic	2799:2805	arg1	wounds					2825:2830	chronic ulcerous infected wounds	2799:2830	chronic ulcerous infected wounds	2799:2830	These results, together with our previous finding that [CEL+KER] composites can be used for the controlled delivery of drugs such as ciprofloxacin, clearly indicate that the [CEL+KER+Ag0NPs] composite possesses all of the required properties for it to be successfully used as a high-performance dressing to treat chronic ulcerous infected wounds.
27998108	6	11	dep	Staphylococcus	1338:1351	arg1	aureus					1353:1358	aureus	1353:1358	aureus	1353:1358	Both composites were found to exhibit excellent antibacterial activity against many bacteria including Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, methicillin-resistant S. aureus (MRSA), and vancomycin-resistant Enterococus faecalis (VRE).
27998108	9	12	theme	embedded	1973:1980	arg1	Ag0NPs					1966:1971	the Ag0NPs	1962:1971	the Ag0NPs embedded in the composite	1962:1997	Experimental results confirm that there was hardly any Ag0NPs release from the [CEL+KER+Ag0NPs] composite, and hence its antimicrobial activity and biocompatibility is due not to any released Ag0NPs but rather entirely to the Ag0NPs embedded in the composite.
27998108	12	13	theme	high-performance	2764:2779	arg1	dressing					2781:2788	a high-performance dressing	2762:2788	a high-performance dressing	2762:2788	These results, together with our previous finding that [CEL+KER] composites can be used for the controlled delivery of drugs such as ciprofloxacin, clearly indicate that the [CEL+KER+Ag0NPs] composite possesses all of the required properties for it to be successfully used as a high-performance dressing to treat chronic ulcerous infected wounds.
27998108	12	13	theme	high-performance	2764:2779	arg1	it					2732:2733	it	2732:2733	it	2732:2733	These results, together with our previous finding that [CEL+KER] composites can be used for the controlled delivery of drugs such as ciprofloxacin, clearly indicate that the [CEL+KER+Ag0NPs] composite possesses all of the required properties for it to be successfully used as a high-performance dressing to treat chronic ulcerous infected wounds.
27998108	1	14	theme	50	226:227	arg1	%					228:228	%	228:228	%	228:228	A novel, simple method was developed to synthesize biocompatible composites containing 50% cellulose (CEL) and 50% keratin (KER) and silver in the form of either ionic (Ag+) or Ag0 nanoparticles (Ag+NPs or Ag0NPs).
27998108	0	15	from	Keratin	85:91	arg1	Composites					55:64	Biocompatible Silver Nanoparticle Composites	21:64	Biocompatible Silver Nanoparticle Composites from Cellulose and Keratin	21:91	One-Pot Synthesis of Biocompatible Silver Nanoparticle Composites from Cellulose and Keratin: Characterization and Antimicrobial Activity.
27998108	0	15	from	Keratin	85:91	arg1	Synthesis					8:16	One-Pot Synthesis	0:16	One-Pot Synthesis of Biocompatible Silver Nanoparticle Composites from Cellulose and Keratin	0:91	One-Pot Synthesis of Biocompatible Silver Nanoparticle Composites from Cellulose and Keratin: Characterization and Antimicrobial Activity.
27998108	0	16	from	Cellulose	71:79	arg1	Composites					55:64	Biocompatible Silver Nanoparticle Composites	21:64	Biocompatible Silver Nanoparticle Composites from Cellulose and Keratin	21:91	One-Pot Synthesis of Biocompatible Silver Nanoparticle Composites from Cellulose and Keratin: Characterization and Antimicrobial Activity.
27998108	0	16	from	Cellulose	71:79	arg1	Synthesis					8:16	One-Pot Synthesis	0:16	One-Pot Synthesis of Biocompatible Silver Nanoparticle Composites from Cellulose and Keratin	0:91	One-Pot Synthesis of Biocompatible Silver Nanoparticle Composites from Cellulose and Keratin: Characterization and Antimicrobial Activity.
27998108	4	17	theme	X-ray	776:780	arg1	XRD					795:797	XRD	795:797	XRD	795:797	The results of spectroscopy [Fourier transform infrared and X-ray diffraction (XRD)] and imaging [scanning electron microscopy (SEM)] measurements confirm that CEL and KER remain chemically intact and homogeneously distributed in the composites.
27998108	4	17	theme	X-ray	776:780	arg1	diffraction					782:792	X-ray diffraction	776:792	X-ray diffraction (XRD)]	776:799	The results of spectroscopy [Fourier transform infrared and X-ray diffraction (XRD)] and imaging [scanning electron microscopy (SEM)] measurements confirm that CEL and KER remain chemically intact and homogeneously distributed in the composites.
27998108	12	18	used	used	2754:2757	arg2	dressing					2781:2788	a high-performance dressing	2762:2788	a high-performance dressing	2762:2788	These results, together with our previous finding that [CEL+KER] composites can be used for the controlled delivery of drugs such as ciprofloxacin, clearly indicate that the [CEL+KER+Ag0NPs] composite possesses all of the required properties for it to be successfully used as a high-performance dressing to treat chronic ulcerous infected wounds.
27998108	12	18	used	used	2754:2757	arg2	it					2732:2733	it	2732:2733	it	2732:2733	These results, together with our previous finding that [CEL+KER] composites can be used for the controlled delivery of drugs such as ciprofloxacin, clearly indicate that the [CEL+KER+Ag0NPs] composite possesses all of the required properties for it to be successfully used as a high-performance dressing to treat chronic ulcerous infected wounds.
27998108	10	19	theme	silver	2123:2128	arg1	content					2130:2136	the same silver content	2114:2136	the same silver content	2114:2136	Both AgClNPs and Ag0NPs were found to be toxic to human fibroblasts at higher concentration (>0.72 mmol), and for the same silver content, the [CEL+KER+AgClNPs] composite is relatively more toxic than the [CEL+KER+Ag0NPs] composite.
27998108	12	20	theme	required	2708:2715	arg1	properties					2717:2726	the required properties	2704:2726	the required properties for it to be successfully used as a high-performance dressing to treat chronic ulcerous infected wounds	2704:2830	These results, together with our previous finding that [CEL+KER] composites can be used for the controlled delivery of drugs such as ciprofloxacin, clearly indicate that the [CEL+KER+Ag0NPs] composite possesses all of the required properties for it to be successfully used as a high-performance dressing to treat chronic ulcerous infected wounds.
27998108	2	21	theme	simple	419:424	arg1	chloride					394:401	butylmethylimmidazolium chloride	370:401	butylmethylimmidazolium chloride ([BMIm+Cl-])	370:414	In this method, butylmethylimmidazolium chloride ([BMIm+Cl-]), a simple ionic liquid, was used as the sole solvent and silver chloride was added to the [BMIm+Cl-] solution of [CEL+KER] during the dissolution process.
27998108	2	21	theme	simple	419:424	arg1	ionic					426:430	a simple ionic liquid	417:437	a simple ionic liquid	417:437	In this method, butylmethylimmidazolium chloride ([BMIm+Cl-]), a simple ionic liquid, was used as the sole solvent and silver chloride was added to the [BMIm+Cl-] solution of [CEL+KER] during the dissolution process.
27998108	7	22	theme	Ag0	1550:1552	arg1	content					1554:1560	the Ag+ or Ag0 content	1539:1560	the Ag+ or Ag0 content in the composites	1539:1578	The antibacterial activity of both composites increases with the Ag+ or Ag0 content in the composites.
27998108	12	23	theme	controlled	2582:2591	arg1	delivery					2593:2600	the controlled delivery	2578:2600	the controlled delivery of drugs such as ciprofloxacin	2578:2631	These results, together with our previous finding that [CEL+KER] composites can be used for the controlled delivery of drugs such as ciprofloxacin, clearly indicate that the [CEL+KER+Ag0NPs] composite possesses all of the required properties for it to be successfully used as a high-performance dressing to treat chronic ulcerous infected wounds.
27998108	11	24	theme	[CEL+KER+Ag0NPs	2309:2323	arg1	composite					2326:2334	the [CEL+KER+Ag0NPs] composite	2305:2334	the [CEL+KER+Ag0NPs] composite	2305:2334	As expected, by lowering the Ag0NPs concentration to 0.48 mmol or less, the [CEL+KER+Ag0NPs] composite can be made biocompatible while still retaining its antimicrobial activity against bacteria such as E. coli, S. aureus, P. aeruginosa, MRSA, and VRE.
27998108	3	25	theme	metallic	667:674	arg1	Ag0					684:686	Ag0	684:686	Ag0	684:686	The silver in the composites can be maintained as ionic silver (Ag+) or completely converted to metallic silver (Ag0) by reducing it with NaBH4.
27998108	3	25	theme	metallic	667:674	arg1	silver					676:681	metallic silver	667:681	metallic silver (Ag0)	667:687	The silver in the composites can be maintained as ionic silver (Ag+) or completely converted to metallic silver (Ag0) by reducing it with NaBH4.
27998108	8	26	theme	same	1607:1610	arg1	bacteria					1612:1619	the same bacteria	1603:1619	the same bacteria	1603:1619	More importantly, for the same bacteria and the same silver content, the [CEL+KER+AgClNPs] composite is relatively more toxic than [CEL+KER+Ag0NPs] composite.
27998108	7	27	theme	Ag+	1543:1545	arg1	content					1554:1560	the Ag+ or Ag0 content	1539:1560	the Ag+ or Ag0 content in the composites	1539:1578	The antibacterial activity of both composites increases with the Ag+ or Ag0 content in the composites.
27998108	10	28	theme	[CEL+KER+AgClNPs	2143:2158	arg1	composite					2161:2169	the [CEL+KER+AgClNPs] composite	2139:2169	the [CEL+KER+AgClNPs] composite	2139:2169	Both AgClNPs and Ag0NPs were found to be toxic to human fibroblasts at higher concentration (>0.72 mmol), and for the same silver content, the [CEL+KER+AgClNPs] composite is relatively more toxic than the [CEL+KER+Ag0NPs] composite.
27998108	10	28	theme	[CEL+KER+AgClNPs	2143:2158	arg1	toxic					2190:2194	toxic	2190:2194	toxic	2190:2194	Both AgClNPs and Ag0NPs were found to be toxic to human fibroblasts at higher concentration (>0.72 mmol), and for the same silver content, the [CEL+KER+AgClNPs] composite is relatively more toxic than the [CEL+KER+Ag0NPs] composite.
27998108	10	29	from	concentration	2078:2090	arg1	toxic					2041:2045	toxic	2041:2045	toxic	2041:2045	Both AgClNPs and Ag0NPs were found to be toxic to human fibroblasts at higher concentration (>0.72 mmol), and for the same silver content, the [CEL+KER+AgClNPs] composite is relatively more toxic than the [CEL+KER+Ag0NPs] composite.
27998108	4	30	dep	transform	753:761	arg1	infrared					763:770	infrared	763:770	transform infrared and X-ray diffraction (XRD)] and imaging [scanning electron microscopy (SEM)] measurements	753:861	The results of spectroscopy [Fourier transform infrared and X-ray diffraction (XRD)] and imaging [scanning electron microscopy (SEM)] measurements confirm that CEL and KER remain chemically intact and homogeneously distributed in the composites.
27998108	1	31	dep	novel	141:145	arg1	simple					148:153	simple	148:153	simple	148:153	A novel, simple method was developed to synthesize biocompatible composites containing 50% cellulose (CEL) and 50% keratin (KER) and silver in the form of either ionic (Ag+) or Ag0 nanoparticles (Ag+NPs or Ag0NPs).
27998108	5	32	theme	Ag+	1125:1127	arg1	form					1164:1167	either Ag+ (in the form of AgClNPs) or Ag0NPs form	1118:1167	either Ag+ (in the form of AgClNPs) or Ag0NPs form with sizes of 27 ± 2 or 9 ± 1 nm, respectively	1118:1214	Powder XRD and SEM results show that the silver in the [CEL+KER+Ag+] and [CEL+KER+Ag0] composites is homogeneously distributed throughout the composites in either Ag+ (in the form of AgClNPs) or Ag0NPs form with sizes of 27 ± 2 or 9 ± 1 nm, respectively.
27998108	8	33	theme	same	1629:1632	arg1	content					1641:1647	the same silver content	1625:1647	the same silver content	1625:1647	More importantly, for the same bacteria and the same silver content, the [CEL+KER+AgClNPs] composite is relatively more toxic than [CEL+KER+Ag0NPs] composite.
27998108	7	34	theme	composites	1513:1522	arg1	activity					1496:1503	The antibacterial activity	1478:1503	The antibacterial activity of both composites	1478:1522	The antibacterial activity of both composites increases with the Ag+ or Ag0 content in the composites.
27998108	4	35	theme	[scanning	813:821	arg1	microscopy					832:841	imaging [scanning electron microscopy	805:841	imaging [scanning electron microscopy (SEM)]	805:848	The results of spectroscopy [Fourier transform infrared and X-ray diffraction (XRD)] and imaging [scanning electron microscopy (SEM)] measurements confirm that CEL and KER remain chemically intact and homogeneously distributed in the composites.
27998108	4	35	theme	[scanning	813:821	arg1	SEM					844:846	SEM	844:846	SEM	844:846	The results of spectroscopy [Fourier transform infrared and X-ray diffraction (XRD)] and imaging [scanning electron microscopy (SEM)] measurements confirm that CEL and KER remain chemically intact and homogeneously distributed in the composites.
27998108	12	36	used	used	2569:2572	arg2	composites					2551:2560	[CEL+KER] composites	2541:2560	[CEL+KER] composites	2541:2560	These results, together with our previous finding that [CEL+KER] composites can be used for the controlled delivery of drugs such as ciprofloxacin, clearly indicate that the [CEL+KER+Ag0NPs] composite possesses all of the required properties for it to be successfully used as a high-performance dressing to treat chronic ulcerous infected wounds.
27998108	2	37	theme	silver	473:478	arg1	chloride					480:487	silver chloride	473:487	silver chloride	473:487	In this method, butylmethylimmidazolium chloride ([BMIm+Cl-]), a simple ionic liquid, was used as the sole solvent and silver chloride was added to the [BMIm+Cl-] solution of [CEL+KER] during the dissolution process.
27998108	0	38	theme	Antimicrobial	115:127	arg1	Activity					129:136	Antimicrobial Activity	115:136	Antimicrobial Activity	115:136	One-Pot Synthesis of Biocompatible Silver Nanoparticle Composites from Cellulose and Keratin: Characterization and Antimicrobial Activity.
27998108	5	39	theme	Powder	962:967	arg1	XRD					969:971	Powder XRD	962:971	Powder XRD	962:971	Powder XRD and SEM results show that the silver in the [CEL+KER+Ag+] and [CEL+KER+Ag0] composites is homogeneously distributed throughout the composites in either Ag+ (in the form of AgClNPs) or Ag0NPs form with sizes of 27 ± 2 or 9 ± 1 nm, respectively.
27998108	0	40	theme	Nanoparticle	42:53	arg1	Composites					55:64	Biocompatible Silver Nanoparticle Composites	21:64	Biocompatible Silver Nanoparticle Composites from Cellulose and Keratin	21:91	One-Pot Synthesis of Biocompatible Silver Nanoparticle Composites from Cellulose and Keratin: Characterization and Antimicrobial Activity.
27998108	0	41	dep	Characterization	94:109	arg1	Synthesis					8:16	One-Pot Synthesis	0:16	One-Pot Synthesis of Biocompatible Silver Nanoparticle Composites from Cellulose and Keratin	0:91	One-Pot Synthesis of Biocompatible Silver Nanoparticle Composites from Cellulose and Keratin: Characterization and Antimicrobial Activity.
27998108	12	42	theme	[CEL+KER	2541:2548	arg1	composites					2551:2560	[CEL+KER] composites	2541:2560	[CEL+KER] composites	2541:2560	These results, together with our previous finding that [CEL+KER] composites can be used for the controlled delivery of drugs such as ciprofloxacin, clearly indicate that the [CEL+KER+Ag0NPs] composite possesses all of the required properties for it to be successfully used as a high-performance dressing to treat chronic ulcerous infected wounds.
27998108	12	43	theme	infected	2816:2823	arg1	wounds					2825:2830	chronic ulcerous infected wounds	2799:2830	chronic ulcerous infected wounds	2799:2830	These results, together with our previous finding that [CEL+KER] composites can be used for the controlled delivery of drugs such as ciprofloxacin, clearly indicate that the [CEL+KER+Ag0NPs] composite possesses all of the required properties for it to be successfully used as a high-performance dressing to treat chronic ulcerous infected wounds.
27998108	0	44	theme	One-Pot	0:6	arg1	Synthesis					8:16	One-Pot Synthesis	0:16	One-Pot Synthesis of Biocompatible Silver Nanoparticle Composites from Cellulose and Keratin	0:91	One-Pot Synthesis of Biocompatible Silver Nanoparticle Composites from Cellulose and Keratin: Characterization and Antimicrobial Activity.
27998108	7	45	theme	antibacterial	1482:1494	arg1	activity					1496:1503	The antibacterial activity	1478:1503	The antibacterial activity of both composites	1478:1522	The antibacterial activity of both composites increases with the Ag+ or Ag0 content in the composites.
27998108	9	46	theme	antimicrobial	1861:1873	arg1	activity					1875:1882	its antimicrobial activity	1857:1882	its antimicrobial activity	1857:1882	Experimental results confirm that there was hardly any Ag0NPs release from the [CEL+KER+Ag0NPs] composite, and hence its antimicrobial activity and biocompatibility is due not to any released Ag0NPs but rather entirely to the Ag0NPs embedded in the composite.
27998108	5	47	theme	AgClNPs	1145:1151	arg1	form					1137:1140	the form	1133:1140	the form of AgClNPs	1133:1151	Powder XRD and SEM results show that the silver in the [CEL+KER+Ag+] and [CEL+KER+Ag0] composites is homogeneously distributed throughout the composites in either Ag+ (in the form of AgClNPs) or Ag0NPs form with sizes of 27 ± 2 or 9 ± 1 nm, respectively.
27998108	12	48	theme	[CEL+KER+Ag0NPs	2660:2674	arg1	composite					2677:2685	the [CEL+KER+Ag0NPs] composite	2656:2685	the [CEL+KER+Ag0NPs] composite	2656:2685	These results, together with our previous finding that [CEL+KER] composites can be used for the controlled delivery of drugs such as ciprofloxacin, clearly indicate that the [CEL+KER+Ag0NPs] composite possesses all of the required properties for it to be successfully used as a high-performance dressing to treat chronic ulcerous infected wounds.
27998108	1	49	dep	ionic	301:305	arg1	Ag+					308:310	Ag+	308:310	Ag+	308:310	A novel, simple method was developed to synthesize biocompatible composites containing 50% cellulose (CEL) and 50% keratin (KER) and silver in the form of either ionic (Ag+) or Ag0 nanoparticles (Ag+NPs or Ag0NPs).
27998108	0	50	theme	Silver	35:40	arg1	Composites					55:64	Biocompatible Silver Nanoparticle Composites	21:64	Biocompatible Silver Nanoparticle Composites from Cellulose and Keratin	21:91	One-Pot Synthesis of Biocompatible Silver Nanoparticle Composites from Cellulose and Keratin: Characterization and Antimicrobial Activity.
27998108	1	51	dep	nanoparticles	320:332	arg1	either					294:299	either	294:299	either	294:299	A novel, simple method was developed to synthesize biocompatible composites containing 50% cellulose (CEL) and 50% keratin (KER) and silver in the form of either ionic (Ag+) or Ag0 nanoparticles (Ag+NPs or Ag0NPs).
27998108	1	51	dep	nanoparticles	320:332	arg1	Ag0NPs					345:350	Ag0NPs	345:350	Ag0NPs	345:350	A novel, simple method was developed to synthesize biocompatible composites containing 50% cellulose (CEL) and 50% keratin (KER) and silver in the form of either ionic (Ag+) or Ag0 nanoparticles (Ag+NPs or Ag0NPs).
27998108	1	51	dep	nanoparticles	320:332	arg1	Ag0					316:318	Ag0	316:318	Ag0	316:318	A novel, simple method was developed to synthesize biocompatible composites containing 50% cellulose (CEL) and 50% keratin (KER) and silver in the form of either ionic (Ag+) or Ag0 nanoparticles (Ag+NPs or Ag0NPs).
27998108	1	51	dep	nanoparticles	320:332	arg1	Ag+NPs					335:340	Ag+NPs	335:340	Ag+NPs	335:340	A novel, simple method was developed to synthesize biocompatible composites containing 50% cellulose (CEL) and 50% keratin (KER) and silver in the form of either ionic (Ag+) or Ag0 nanoparticles (Ag+NPs or Ag0NPs).
27998108	1	51	dep	nanoparticles	320:332	arg1	nanoparticles					320:332	either ionic (Ag+) or Ag0 nanoparticles	294:332	either ionic (Ag+) or Ag0 nanoparticles (Ag+NPs or Ag0NPs)	294:351	A novel, simple method was developed to synthesize biocompatible composites containing 50% cellulose (CEL) and 50% keratin (KER) and silver in the form of either ionic (Ag+) or Ag0 nanoparticles (Ag+NPs or Ag0NPs).
27998108	2	52	used	used	444:447	arg2	solvent					461:467	the sole solvent	452:467	the sole solvent	452:467	In this method, butylmethylimmidazolium chloride ([BMIm+Cl-]), a simple ionic liquid, was used as the sole solvent and silver chloride was added to the [BMIm+Cl-] solution of [CEL+KER] during the dissolution process.
27998108	2	52	used	used	444:447	arg2	[BMIm+Cl-					404:412	[BMIm+Cl-	404:412	[BMIm+Cl-	404:412	In this method, butylmethylimmidazolium chloride ([BMIm+Cl-]), a simple ionic liquid, was used as the sole solvent and silver chloride was added to the [BMIm+Cl-] solution of [CEL+KER] during the dissolution process.
27998108	2	52	used	used	444:447	arg2	chloride					394:401	butylmethylimmidazolium chloride	370:401	butylmethylimmidazolium chloride ([BMIm+Cl-])	370:414	In this method, butylmethylimmidazolium chloride ([BMIm+Cl-]), a simple ionic liquid, was used as the sole solvent and silver chloride was added to the [BMIm+Cl-] solution of [CEL+KER] during the dissolution process.
27998108	2	52	used	used	444:447	arg2	ionic					426:430	a simple ionic liquid	417:437	a simple ionic liquid	417:437	In this method, butylmethylimmidazolium chloride ([BMIm+Cl-]), a simple ionic liquid, was used as the sole solvent and silver chloride was added to the [BMIm+Cl-] solution of [CEL+KER] during the dissolution process.
27998108	0	53	theme	Composites	55:64	arg1	Synthesis					8:16	One-Pot Synthesis	0:16	One-Pot Synthesis of Biocompatible Silver Nanoparticle Composites from Cellulose and Keratin	0:91	One-Pot Synthesis of Biocompatible Silver Nanoparticle Composites from Cellulose and Keratin: Characterization and Antimicrobial Activity.
27998108	9	54	from	composite	1989:1997	arg1	embedded					1973:1980	embedded	1973:1980	embedded	1973:1980	Experimental results confirm that there was hardly any Ag0NPs release from the [CEL+KER+Ag0NPs] composite, and hence its antimicrobial activity and biocompatibility is due not to any released Ag0NPs but rather entirely to the Ag0NPs embedded in the composite.
27998108	6	55	theme	many	1296:1299	arg1	bacteria					1301:1308	many bacteria	1296:1308	many bacteria including Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, methicillin-resistant S. aureus (MRSA), and vancomycin-resistant Enterococus faecalis (VRE)	1296:1475	Both composites were found to exhibit excellent antibacterial activity against many bacteria including Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, methicillin-resistant S. aureus (MRSA), and vancomycin-resistant Enterococus faecalis (VRE).
27998108	1	56	contain	containing	215:224	arg2	cellulose					230:238	cellulose	230:238	cellulose (CEL)	230:244	A novel, simple method was developed to synthesize biocompatible composites containing 50% cellulose (CEL) and 50% keratin (KER) and silver in the form of either ionic (Ag+) or Ag0 nanoparticles (Ag+NPs or Ag0NPs).
27998108	1	56	contain	containing	215:224	arg2	KER					263:265	KER	263:265	KER	263:265	A novel, simple method was developed to synthesize biocompatible composites containing 50% cellulose (CEL) and 50% keratin (KER) and silver in the form of either ionic (Ag+) or Ag0 nanoparticles (Ag+NPs or Ag0NPs).
27998108	1	56	contain	containing	215:224	arg1	composites					204:213	biocompatible composites	190:213	biocompatible composites containing 50% cellulose (CEL) and 50% keratin (KER) and silver in the form of either ionic (Ag+) or Ag0 nanoparticles (Ag+NPs or Ag0NPs)	190:351	A novel, simple method was developed to synthesize biocompatible composites containing 50% cellulose (CEL) and 50% keratin (KER) and silver in the form of either ionic (Ag+) or Ag0 nanoparticles (Ag+NPs or Ag0NPs).
27998108	1	56	contain	containing	215:224	arg2	silver					272:277	silver	272:277	silver	272:277	A novel, simple method was developed to synthesize biocompatible composites containing 50% cellulose (CEL) and 50% keratin (KER) and silver in the form of either ionic (Ag+) or Ag0 nanoparticles (Ag+NPs or Ag0NPs).
27998108	1	56	contain	containing	215:224	arg2	keratin					254:260	50% keratin	250:260	50% keratin (KER)	250:266	A novel, simple method was developed to synthesize biocompatible composites containing 50% cellulose (CEL) and 50% keratin (KER) and silver in the form of either ionic (Ag+) or Ag0 nanoparticles (Ag+NPs or Ag0NPs).
27998108	1	56	contain	containing	215:224	arg2	CEL					241:243	CEL	241:243	CEL	241:243	A novel, simple method was developed to synthesize biocompatible composites containing 50% cellulose (CEL) and 50% keratin (KER) and silver in the form of either ionic (Ag+) or Ag0 nanoparticles (Ag+NPs or Ag0NPs).
27998108	5	57	from	silver	1003:1008	arg1	composites					1049:1058	the [CEL+KER+Ag+] and [CEL+KER+Ag0] composites	1013:1058	composites	1049:1058	Powder XRD and SEM results show that the silver in the [CEL+KER+Ag+] and [CEL+KER+Ag0] composites is homogeneously distributed throughout the composites in either Ag+ (in the form of AgClNPs) or Ag0NPs form with sizes of 27 ± 2 or 9 ± 1 nm, respectively.
27998108	5	57	from	silver	1003:1008	arg1	[CEL+KER+Ag+					1017:1028	[CEL+KER+Ag+	1017:1028	[CEL+KER+Ag+	1017:1028	Powder XRD and SEM results show that the silver in the [CEL+KER+Ag+] and [CEL+KER+Ag0] composites is homogeneously distributed throughout the composites in either Ag+ (in the form of AgClNPs) or Ag0NPs form with sizes of 27 ± 2 or 9 ± 1 nm, respectively.
27998108	6	58	theme	excellent	1255:1263	arg1	activity					1279:1286	excellent antibacterial activity	1255:1286	excellent antibacterial activity against many bacteria including Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, methicillin-resistant S. aureus (MRSA), and vancomycin-resistant Enterococus faecalis (VRE)	1255:1475	Both composites were found to exhibit excellent antibacterial activity against many bacteria including Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, methicillin-resistant S. aureus (MRSA), and vancomycin-resistant Enterococus faecalis (VRE).
27998108	1	59	theme	50	250:251	arg1	%					252:252	%	252:252	%	252:252	A novel, simple method was developed to synthesize biocompatible composites containing 50% cellulose (CEL) and 50% keratin (KER) and silver in the form of either ionic (Ag+) or Ag0 nanoparticles (Ag+NPs or Ag0NPs).
27998108	10	60	theme	higher	2071:2076	arg1	concentration					2078:2090	higher concentration	2071:2090	higher concentration (>0.72 mmol)	2071:2103	Both AgClNPs and Ag0NPs were found to be toxic to human fibroblasts at higher concentration (>0.72 mmol), and for the same silver content, the [CEL+KER+AgClNPs] composite is relatively more toxic than the [CEL+KER+Ag0NPs] composite.
27998108	10	60	theme	higher	2071:2076	arg1	mmol					2099:2102	>0.72 mmol	2093:2102	>0.72 mmol	2093:2102	Both AgClNPs and Ag0NPs were found to be toxic to human fibroblasts at higher concentration (>0.72 mmol), and for the same silver content, the [CEL+KER+AgClNPs] composite is relatively more toxic than the [CEL+KER+Ag0NPs] composite.
27998108	4	61	theme	spectroscopy	731:742	arg1	[Fourier					744:751	spectroscopy [Fourier	731:751	spectroscopy [Fourier transform infrared and X-ray diffraction (XRD)] and imaging [scanning electron microscopy (SEM)] measurements	731:861	The results of spectroscopy [Fourier transform infrared and X-ray diffraction (XRD)] and imaging [scanning electron microscopy (SEM)] measurements confirm that CEL and KER remain chemically intact and homogeneously distributed in the composites.
27998108	3	62	theme	ionic	621:625	arg1	Ag+					635:637	Ag+	635:637	Ag+	635:637	The silver in the composites can be maintained as ionic silver (Ag+) or completely converted to metallic silver (Ag0) by reducing it with NaBH4.
27998108	3	62	theme	ionic	621:625	arg1	silver					627:632	ionic silver	621:632	ionic silver (Ag+)	621:638	The silver in the composites can be maintained as ionic silver (Ag+) or completely converted to metallic silver (Ag0) by reducing it with NaBH4.
27998108	3	62	theme	ionic	621:625	arg1	silver					575:580	The silver	571:580	The silver in the composites	571:598	The silver in the composites can be maintained as ionic silver (Ag+) or completely converted to metallic silver (Ag0) by reducing it with NaBH4.
27998108	12	63	theme	ulcerous	2807:2814	arg1	wounds					2825:2830	chronic ulcerous infected wounds	2799:2830	chronic ulcerous infected wounds	2799:2830	These results, together with our previous finding that [CEL+KER] composites can be used for the controlled delivery of drugs such as ciprofloxacin, clearly indicate that the [CEL+KER+Ag0NPs] composite possesses all of the required properties for it to be successfully used as a high-performance dressing to treat chronic ulcerous infected wounds.
27998108	11	64	theme	antimicrobial	2388:2400	arg1	activity					2402:2409	its antimicrobial activity	2384:2409	its antimicrobial activity against bacteria such as E. coli, S. aureus, P. aeruginosa, MRSA, and VRE	2384:2483	As expected, by lowering the Ag0NPs concentration to 0.48 mmol or less, the [CEL+KER+Ag0NPs] composite can be made biocompatible while still retaining its antimicrobial activity against bacteria such as E. coli, S. aureus, P. aeruginosa, MRSA, and VRE.
27998108	9	65	from	embedded	1973:1980	arg1	composite					1989:1997	composite	1989:1997	composite	1989:1997	Experimental results confirm that there was hardly any Ag0NPs release from the [CEL+KER+Ag0NPs] composite, and hence its antimicrobial activity and biocompatibility is due not to any released Ag0NPs but rather entirely to the Ag0NPs embedded in the composite.
27998108	1	66	theme	biocompatible	190:202	arg1	composites					204:213	biocompatible composites	190:213	biocompatible composites containing 50% cellulose (CEL) and 50% keratin (KER) and silver in the form of either ionic (Ag+) or Ag0 nanoparticles (Ag+NPs or Ag0NPs)	190:351	A novel, simple method was developed to synthesize biocompatible composites containing 50% cellulose (CEL) and 50% keratin (KER) and silver in the form of either ionic (Ag+) or Ag0 nanoparticles (Ag+NPs or Ag0NPs).
27998108	6	67	dep	Pseudomonas	1361:1371	arg1	aeruginosa					1373:1382	aeruginosa	1373:1382	aeruginosa	1373:1382	Both composites were found to exhibit excellent antibacterial activity against many bacteria including Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, methicillin-resistant S. aureus (MRSA), and vancomycin-resistant Enterococus faecalis (VRE).
27998108	7	68	from	content	1554:1560	arg1	composites					1569:1578	the composites	1565:1578	the composites	1565:1578	The antibacterial activity of both composites increases with the Ag+ or Ag0 content in the composites.
27998108	9	69	theme	Experimental	1740:1751	arg1	results					1753:1759	Experimental results	1740:1759	Experimental results	1740:1759	Experimental results confirm that there was hardly any Ag0NPs release from the [CEL+KER+Ag0NPs] composite, and hence its antimicrobial activity and biocompatibility is due not to any released Ag0NPs but rather entirely to the Ag0NPs embedded in the composite.
27998108	9	70	theme	[CEL+KER+Ag0NPs	1819:1833	arg1	composite					1836:1844	the [CEL+KER+Ag0NPs] composite	1815:1844	the [CEL+KER+Ag0NPs] composite	1815:1844	Experimental results confirm that there was hardly any Ag0NPs release from the [CEL+KER+Ag0NPs] composite, and hence its antimicrobial activity and biocompatibility is due not to any released Ag0NPs but rather entirely to the Ag0NPs embedded in the composite.
27998108	2	71	theme	sole	456:459	arg1	chloride					394:401	butylmethylimmidazolium chloride	370:401	butylmethylimmidazolium chloride ([BMIm+Cl-])	370:414	In this method, butylmethylimmidazolium chloride ([BMIm+Cl-]), a simple ionic liquid, was used as the sole solvent and silver chloride was added to the [BMIm+Cl-] solution of [CEL+KER] during the dissolution process.
27998108	2	71	theme	sole	456:459	arg1	solvent					461:467	the sole solvent	452:467	the sole solvent	452:467	In this method, butylmethylimmidazolium chloride ([BMIm+Cl-]), a simple ionic liquid, was used as the sole solvent and silver chloride was added to the [BMIm+Cl-] solution of [CEL+KER] during the dissolution process.
27998108	1	72	theme	%	228:228	arg1	CEL					241:243	CEL	241:243	CEL	241:243	A novel, simple method was developed to synthesize biocompatible composites containing 50% cellulose (CEL) and 50% keratin (KER) and silver in the form of either ionic (Ag+) or Ag0 nanoparticles (Ag+NPs or Ag0NPs).
27998108	1	72	theme	%	228:228	arg1	cellulose					230:238	cellulose	230:238	cellulose (CEL)	230:244	A novel, simple method was developed to synthesize biocompatible composites containing 50% cellulose (CEL) and 50% keratin (KER) and silver in the form of either ionic (Ag+) or Ag0 nanoparticles (Ag+NPs or Ag0NPs).
27998108	1	73	theme	novel	141:145	arg1	method					155:160	A novel, simple method	139:160	A novel, simple method	139:160	A novel, simple method was developed to synthesize biocompatible composites containing 50% cellulose (CEL) and 50% keratin (KER) and silver in the form of either ionic (Ag+) or Ag0 nanoparticles (Ag+NPs or Ag0NPs).
27998108	10	74	theme	same	2118:2121	arg1	content					2130:2136	the same silver content	2114:2136	the same silver content	2114:2136	Both AgClNPs and Ag0NPs were found to be toxic to human fibroblasts at higher concentration (>0.72 mmol), and for the same silver content, the [CEL+KER+AgClNPs] composite is relatively more toxic than the [CEL+KER+Ag0NPs] composite.
27998108	9	75	theme	Ag0NPs	1795:1800	arg1	release					1802:1808	any Ag0NPs release	1791:1808	any Ag0NPs release from the [CEL+KER+Ag0NPs] composite	1791:1844	Experimental results confirm that there was hardly any Ag0NPs release from the [CEL+KER+Ag0NPs] composite, and hence its antimicrobial activity and biocompatibility is due not to any released Ag0NPs but rather entirely to the Ag0NPs embedded in the composite.
27998108	5	76	with	form	1164:1167	arg1	sizes					1174:1178	sizes	1174:1178	sizes of 27 ± 2 or 9 ± 1 nm, respectively	1174:1214	Powder XRD and SEM results show that the silver in the [CEL+KER+Ag+] and [CEL+KER+Ag0] composites is homogeneously distributed throughout the composites in either Ag+ (in the form of AgClNPs) or Ag0NPs form with sizes of 27 ± 2 or 9 ± 1 nm, respectively.
27998108	6	77	dep	methicillin-resistant	1385:1405	arg1	MRSA					1418:1421	MRSA	1418:1421	MRSA	1418:1421	Both composites were found to exhibit excellent antibacterial activity against many bacteria including Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, methicillin-resistant S. aureus (MRSA), and vancomycin-resistant Enterococus faecalis (VRE).
27998108	6	77	dep	methicillin-resistant	1385:1405	arg1	aureus					1410:1415	S. aureus	1407:1415	S. aureus (MRSA)	1407:1422	Both composites were found to exhibit excellent antibacterial activity against many bacteria including Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, methicillin-resistant S. aureus (MRSA), and vancomycin-resistant Enterococus faecalis (VRE).
27998108	4	78	theme	electron	823:830	arg1	microscopy					832:841	imaging [scanning electron microscopy	805:841	imaging [scanning electron microscopy (SEM)]	805:848	The results of spectroscopy [Fourier transform infrared and X-ray diffraction (XRD)] and imaging [scanning electron microscopy (SEM)] measurements confirm that CEL and KER remain chemically intact and homogeneously distributed in the composites.
27998108	4	78	theme	electron	823:830	arg1	SEM					844:846	SEM	844:846	SEM	844:846	The results of spectroscopy [Fourier transform infrared and X-ray diffraction (XRD)] and imaging [scanning electron microscopy (SEM)] measurements confirm that CEL and KER remain chemically intact and homogeneously distributed in the composites.
27998108	4	79	theme	imaging	805:811	arg1	microscopy					832:841	imaging [scanning electron microscopy	805:841	imaging [scanning electron microscopy (SEM)]	805:848	The results of spectroscopy [Fourier transform infrared and X-ray diffraction (XRD)] and imaging [scanning electron microscopy (SEM)] measurements confirm that CEL and KER remain chemically intact and homogeneously distributed in the composites.
27998108	4	79	theme	imaging	805:811	arg1	SEM					844:846	SEM	844:846	SEM	844:846	The results of spectroscopy [Fourier transform infrared and X-ray diffraction (XRD)] and imaging [scanning electron microscopy (SEM)] measurements confirm that CEL and KER remain chemically intact and homogeneously distributed in the composites.
27998108	5	80	theme	SEM	977:979	arg1	results					981:987	Powder XRD and SEM results	962:987	Powder XRD and SEM results	962:987	Powder XRD and SEM results show that the silver in the [CEL+KER+Ag+] and [CEL+KER+Ag0] composites is homogeneously distributed throughout the composites in either Ag+ (in the form of AgClNPs) or Ag0NPs form with sizes of 27 ± 2 or 9 ± 1 nm, respectively.
27998108	0	81	from	Synthesis	8:16	arg1	Keratin					85:91	Keratin	85:91	Keratin	85:91	One-Pot Synthesis of Biocompatible Silver Nanoparticle Composites from Cellulose and Keratin: Characterization and Antimicrobial Activity.
27998108	0	81	from	Synthesis	8:16	arg1	Cellulose					71:79	Cellulose	71:79	Cellulose	71:79	One-Pot Synthesis of Biocompatible Silver Nanoparticle Composites from Cellulose and Keratin: Characterization and Antimicrobial Activity.
27998108	2	82	theme	dissolution	550:560	arg1	process					562:568	the dissolution process	546:568	the dissolution process	546:568	In this method, butylmethylimmidazolium chloride ([BMIm+Cl-]), a simple ionic liquid, was used as the sole solvent and silver chloride was added to the [BMIm+Cl-] solution of [CEL+KER] during the dissolution process.
27998108	5	83	theme	Ag0NPs	1157:1162	arg1	form					1164:1167	either Ag+ (in the form of AgClNPs) or Ag0NPs form	1118:1167	either Ag+ (in the form of AgClNPs) or Ag0NPs form with sizes of 27 ± 2 or 9 ± 1 nm, respectively	1118:1214	Powder XRD and SEM results show that the silver in the [CEL+KER+Ag+] and [CEL+KER+Ag0] composites is homogeneously distributed throughout the composites in either Ag+ (in the form of AgClNPs) or Ag0NPs form with sizes of 27 ± 2 or 9 ± 1 nm, respectively.
27998108	2	84	theme	liquid	432:437	arg1	chloride					394:401	butylmethylimmidazolium chloride	370:401	butylmethylimmidazolium chloride ([BMIm+Cl-])	370:414	In this method, butylmethylimmidazolium chloride ([BMIm+Cl-]), a simple ionic liquid, was used as the sole solvent and silver chloride was added to the [BMIm+Cl-] solution of [CEL+KER] during the dissolution process.
27998108	2	84	theme	liquid	432:437	arg1	ionic					426:430	a simple ionic liquid	417:437	a simple ionic liquid	417:437	In this method, butylmethylimmidazolium chloride ([BMIm+Cl-]), a simple ionic liquid, was used as the sole solvent and silver chloride was added to the [BMIm+Cl-] solution of [CEL+KER] during the dissolution process.
27998108	11	85	theme	Ag0NPs	2262:2267	arg1	concentration					2269:2281	the Ag0NPs concentration	2258:2281	the Ag0NPs concentration to 0.48 mmol or less	2258:2302	As expected, by lowering the Ag0NPs concentration to 0.48 mmol or less, the [CEL+KER+Ag0NPs] composite can be made biocompatible while still retaining its antimicrobial activity against bacteria such as E. coli, S. aureus, P. aeruginosa, MRSA, and VRE.
27998108	10	86	theme	human	2050:2054	arg1	fibroblasts					2056:2066	human fibroblasts	2050:2066	human fibroblasts	2050:2066	Both AgClNPs and Ag0NPs were found to be toxic to human fibroblasts at higher concentration (>0.72 mmol), and for the same silver content, the [CEL+KER+AgClNPs] composite is relatively more toxic than the [CEL+KER+Ag0NPs] composite.
27998108	5	87	theme	XRD	969:971	arg1	results					981:987	Powder XRD and SEM results	962:987	Powder XRD and SEM results	962:987	Powder XRD and SEM results show that the silver in the [CEL+KER+Ag+] and [CEL+KER+Ag0] composites is homogeneously distributed throughout the composites in either Ag+ (in the form of AgClNPs) or Ag0NPs form with sizes of 27 ± 2 or 9 ± 1 nm, respectively.
27998108	12	88	contain	possesses	2687:2695	arg2	all					2697:2699	all	2697:2699	all	2697:2699	These results, together with our previous finding that [CEL+KER] composites can be used for the controlled delivery of drugs such as ciprofloxacin, clearly indicate that the [CEL+KER+Ag0NPs] composite possesses all of the required properties for it to be successfully used as a high-performance dressing to treat chronic ulcerous infected wounds.
27998108	12	88	contain	possesses	2687:2695	arg1	composite					2677:2685	the [CEL+KER+Ag0NPs] composite	2656:2685	the [CEL+KER+Ag0NPs] composite	2656:2685	These results, together with our previous finding that [CEL+KER] composites can be used for the controlled delivery of drugs such as ciprofloxacin, clearly indicate that the [CEL+KER+Ag0NPs] composite possesses all of the required properties for it to be successfully used as a high-performance dressing to treat chronic ulcerous infected wounds.
27998108	12	88	contain	possesses	2687:2695	arg2	properties					2717:2726	the required properties	2704:2726	the required properties for it to be successfully used as a high-performance dressing to treat chronic ulcerous infected wounds	2704:2830	These results, together with our previous finding that [CEL+KER] composites can be used for the controlled delivery of drugs such as ciprofloxacin, clearly indicate that the [CEL+KER+Ag0NPs] composite possesses all of the required properties for it to be successfully used as a high-performance dressing to treat chronic ulcerous infected wounds.
27998108	8	89	theme	silver	1634:1639	arg1	content					1641:1647	the same silver content	1625:1647	the same silver content	1625:1647	More importantly, for the same bacteria and the same silver content, the [CEL+KER+AgClNPs] composite is relatively more toxic than [CEL+KER+Ag0NPs] composite.
27998108	2	90	theme	butylmethylimmidazolium	370:392	arg1	ionic					426:430	a simple ionic liquid	417:437	a simple ionic liquid	417:437	In this method, butylmethylimmidazolium chloride ([BMIm+Cl-]), a simple ionic liquid, was used as the sole solvent and silver chloride was added to the [BMIm+Cl-] solution of [CEL+KER] during the dissolution process.
27998108	2	90	theme	butylmethylimmidazolium	370:392	arg1	solvent					461:467	the sole solvent	452:467	the sole solvent	452:467	In this method, butylmethylimmidazolium chloride ([BMIm+Cl-]), a simple ionic liquid, was used as the sole solvent and silver chloride was added to the [BMIm+Cl-] solution of [CEL+KER] during the dissolution process.
27998108	2	90	theme	butylmethylimmidazolium	370:392	arg1	chloride					394:401	butylmethylimmidazolium chloride	370:401	butylmethylimmidazolium chloride ([BMIm+Cl-])	370:414	In this method, butylmethylimmidazolium chloride ([BMIm+Cl-]), a simple ionic liquid, was used as the sole solvent and silver chloride was added to the [BMIm+Cl-] solution of [CEL+KER] during the dissolution process.
27998108	2	90	theme	butylmethylimmidazolium	370:392	arg1	[BMIm+Cl-					404:412	[BMIm+Cl-	404:412	[BMIm+Cl-	404:412	In this method, butylmethylimmidazolium chloride ([BMIm+Cl-]), a simple ionic liquid, was used as the sole solvent and silver chloride was added to the [BMIm+Cl-] solution of [CEL+KER] during the dissolution process.
27998108	0	91	theme	Biocompatible	21:33	arg1	Composites					55:64	Biocompatible Silver Nanoparticle Composites	21:64	Biocompatible Silver Nanoparticle Composites from Cellulose and Keratin	21:91	One-Pot Synthesis of Biocompatible Silver Nanoparticle Composites from Cellulose and Keratin: Characterization and Antimicrobial Activity.
27998108	1	92	dep	either	294:299	arg1	ionic					301:305	ionic	301:305	ionic	301:305	A novel, simple method was developed to synthesize biocompatible composites containing 50% cellulose (CEL) and 50% keratin (KER) and silver in the form of either ionic (Ag+) or Ag0 nanoparticles (Ag+NPs or Ag0NPs).
27998108	4	93	dep	[Fourier	744:751	arg1	transform					753:761	transform	753:761	transform infrared and X-ray diffraction (XRD)] and imaging [scanning electron microscopy (SEM)] measurements	753:861	The results of spectroscopy [Fourier transform infrared and X-ray diffraction (XRD)] and imaging [scanning electron microscopy (SEM)] measurements confirm that CEL and KER remain chemically intact and homogeneously distributed in the composites.
27998108	4	94	dep	diffraction	782:792	arg1	measurements					850:861	measurements	850:861	measurements	850:861	The results of spectroscopy [Fourier transform infrared and X-ray diffraction (XRD)] and imaging [scanning electron microscopy (SEM)] measurements confirm that CEL and KER remain chemically intact and homogeneously distributed in the composites.
27998108	12	95	theme	previous	2519:2526	arg1	finding					2528:2534	our previous finding that [CEL+KER] composites can be used for the controlled delivery of drugs such as ciprofloxacin	2515:2631	our previous finding that [CEL+KER] composites can be used for the controlled delivery of drugs such as ciprofloxacin	2515:2631	These results, together with our previous finding that [CEL+KER] composites can be used for the controlled delivery of drugs such as ciprofloxacin, clearly indicate that the [CEL+KER+Ag0NPs] composite possesses all of the required properties for it to be successfully used as a high-performance dressing to treat chronic ulcerous infected wounds.
27998108	5	96	theme	±	1195:1195	arg1	nm					1199:1200	27 ± 2 or 9 ± 1 nm	1183:1200	27 ± 2 or 9 ± 1 nm	1183:1200	Powder XRD and SEM results show that the silver in the [CEL+KER+Ag+] and [CEL+KER+Ag0] composites is homogeneously distributed throughout the composites in either Ag+ (in the form of AgClNPs) or Ag0NPs form with sizes of 27 ± 2 or 9 ± 1 nm, respectively.
27998108	9	97	from	composite	1836:1844	arg1	release					1802:1808	any Ag0NPs release	1791:1808	any Ag0NPs release from the [CEL+KER+Ag0NPs] composite	1791:1844	Experimental results confirm that there was hardly any Ag0NPs release from the [CEL+KER+Ag0NPs] composite, and hence its antimicrobial activity and biocompatibility is due not to any released Ag0NPs but rather entirely to the Ag0NPs embedded in the composite.
27998108	9	98	theme	released	1923:1930	arg1	Ag0NPs					1932:1937	any released Ag0NPs	1919:1937	any released Ag0NPs	1919:1937	Experimental results confirm that there was hardly any Ag0NPs release from the [CEL+KER+Ag0NPs] composite, and hence its antimicrobial activity and biocompatibility is due not to any released Ag0NPs but rather entirely to the Ag0NPs embedded in the composite.
27998108	5	99	theme	±	1186:1186	arg1	nm					1199:1200	27 ± 2 or 9 ± 1 nm	1183:1200	27 ± 2 or 9 ± 1 nm	1183:1200	Powder XRD and SEM results show that the silver in the [CEL+KER+Ag+] and [CEL+KER+Ag0] composites is homogeneously distributed throughout the composites in either Ag+ (in the form of AgClNPs) or Ag0NPs form with sizes of 27 ± 2 or 9 ± 1 nm, respectively.
27998108	12	100	theme	drugs	2605:2609	arg1	delivery					2593:2600	the controlled delivery	2578:2600	the controlled delivery of drugs such as ciprofloxacin	2578:2631	These results, together with our previous finding that [CEL+KER] composites can be used for the controlled delivery of drugs such as ciprofloxacin, clearly indicate that the [CEL+KER+Ag0NPs] composite possesses all of the required properties for it to be successfully used as a high-performance dressing to treat chronic ulcerous infected wounds.
27998108	6	101	theme	antibacterial	1265:1277	arg1	activity					1279:1286	excellent antibacterial activity	1255:1286	excellent antibacterial activity against many bacteria including Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, methicillin-resistant S. aureus (MRSA), and vancomycin-resistant Enterococus faecalis (VRE)	1255:1475	Both composites were found to exhibit excellent antibacterial activity against many bacteria including Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, methicillin-resistant S. aureus (MRSA), and vancomycin-resistant Enterococus faecalis (VRE).
25439889	2	0	contain	containing	265:274	arg2	components					293:302	three major wood components	276:302	three major wood components	276:302	The controllable properties of wood mimetic composites containing three major wood components are useful for enzyme immobilization.
25439889	2	0	contain	containing	265:274	arg1	composites					254:263	wood mimetic composites	241:263	wood mimetic composites containing three major wood components	241:302	The controllable properties of wood mimetic composites containing three major wood components are useful for enzyme immobilization.
25439889	5	1	theme	pH	763:764	arg1	stability					766:774	thermal and pH stability	751:774	stability	766:774	The lipase entrapped in various wood mimetic beads showed increased thermal and pH stability.
25439889	5	2	theme	wood	715:718	arg1	beads					728:732	various wood mimetic beads	707:732	various wood mimetic beads	707:732	The lipase entrapped in various wood mimetic beads showed increased thermal and pH stability.
25439889	6	3	theme	free	896:899	arg1	lipase					901:906	free lipase	896:906	free lipase during incubation	896:924	The half-life times of lipase entrapped in cellulose/alkali lignin hydrogel were 31- and 82-times higher than those of free lipase during incubation under denaturing conditions of high temperature and low pH, respectively.
25439889	6	4	theme	lipase	800:805	arg1	higher					875:880	higher	875:880	higher	875:880	The half-life times of lipase entrapped in cellulose/alkali lignin hydrogel were 31- and 82-times higher than those of free lipase during incubation under denaturing conditions of high temperature and low pH, respectively.
25439889	6	4	theme	lipase	800:805	arg1	times					791:795	The half-life times	777:795	The half-life times of lipase entrapped in cellulose/alkali lignin hydrogel	777:851	The half-life times of lipase entrapped in cellulose/alkali lignin hydrogel were 31- and 82-times higher than those of free lipase during incubation under denaturing conditions of high temperature and low pH, respectively.
25439889	1	5	theme	Wood	55:58	arg1	hydrogels					86:94	Wood component-based composite hydrogels	55:94	Wood component-based composite hydrogels	55:94	Wood component-based composite hydrogels have potential applications in biomedical fields owing to their low cost, biodegradability, and biocompatibility.
25439889	5	6	theme	mimetic	720:726	arg1	beads					728:732	various wood mimetic beads	707:732	various wood mimetic beads	707:732	The lipase entrapped in various wood mimetic beads showed increased thermal and pH stability.
25439889	3	7	dep	[Emim	496:500	arg1	[Ac					502:504	[Ac	502:504	[Ac	502:504	Here, lipase from Candida rugosa was entrapped in wood mimetic beads containing cellulose, xylan, and lignin by dissolving wood components with lipase in [Emim][Ac], followed by reconstitution.
25439889	1	8	theme	component-based	60:74	arg1	hydrogels					86:94	Wood component-based composite hydrogels	55:94	Wood component-based composite hydrogels	55:94	Wood component-based composite hydrogels have potential applications in biomedical fields owing to their low cost, biodegradability, and biocompatibility.
25439889	6	9	theme	pH	982:983	arg1	conditions					943:952	denaturing conditions	932:952	denaturing conditions of high temperature and low pH	932:983	The half-life times of lipase entrapped in cellulose/alkali lignin hydrogel were 31- and 82-times higher than those of free lipase during incubation under denaturing conditions of high temperature and low pH, respectively.
25439889	7	10	theme	bioelectronic	1186:1198	arg1	fields					1218:1223	the biomedical, bioelectronic, and biocatalytic fields	1170:1223	the biomedical, bioelectronic, and biocatalytic fields	1170:1223	Owing to their biocompatibility, biodegradability, and controllable properties, wood mimetic hydrogel beads can be used to immobilize various enzymes for applications in the biomedical, bioelectronic, and biocatalytic fields.
25439889	1	11	theme	composite	76:84	arg1	hydrogels					86:94	Wood component-based composite hydrogels	55:94	Wood component-based composite hydrogels	55:94	Wood component-based composite hydrogels have potential applications in biomedical fields owing to their low cost, biodegradability, and biocompatibility.
25439889	4	12	theme	5:3:2	590:594	arg1	ratio					596:600	a 5:3:2 ratio	588:600	a 5:3:2 ratio	588:600	Lipase entrapped in cellulose/xylan/lignin beads in a 5:3:2 ratio showed the highest activity; this ratio is very similar to that in natural wood.
25439889	6	13	theme	half-life	781:789	arg1	higher					875:880	higher	875:880	higher	875:880	The half-life times of lipase entrapped in cellulose/alkali lignin hydrogel were 31- and 82-times higher than those of free lipase during incubation under denaturing conditions of high temperature and low pH, respectively.
25439889	6	13	theme	half-life	781:789	arg1	times					791:795	The half-life times	777:795	The half-life times of lipase entrapped in cellulose/alkali lignin hydrogel	777:851	The half-life times of lipase entrapped in cellulose/alkali lignin hydrogel were 31- and 82-times higher than those of free lipase during incubation under denaturing conditions of high temperature and low pH, respectively.
25439889	4	14	from	beads	579:583	arg1	ratio					596:600	a 5:3:2 ratio	588:600	a 5:3:2 ratio	588:600	Lipase entrapped in cellulose/xylan/lignin beads in a 5:3:2 ratio showed the highest activity; this ratio is very similar to that in natural wood.
25439889	0	15	theme	mimetic	5:11	arg1	hydrogel					13:20	Wood mimetic hydrogel	0:20	Wood mimetic hydrogel	0:20	Wood mimetic hydrogel beads for enzyme immobilization.
25439889	7	16	theme	mimetic	1085:1091	arg1	beads					1102:1106	wood mimetic hydrogel beads	1080:1106	wood mimetic hydrogel beads	1080:1106	Owing to their biocompatibility, biodegradability, and controllable properties, wood mimetic hydrogel beads can be used to immobilize various enzymes for applications in the biomedical, bioelectronic, and biocatalytic fields.
25439889	6	17	theme	low	978:980	arg1	pH					982:983	low pH	978:983	low pH	978:983	The half-life times of lipase entrapped in cellulose/alkali lignin hydrogel were 31- and 82-times higher than those of free lipase during incubation under denaturing conditions of high temperature and low pH, respectively.
25439889	7	18	theme	biomedical	1174:1183	arg1	fields					1218:1223	the biomedical, bioelectronic, and biocatalytic fields	1170:1223	the biomedical, bioelectronic, and biocatalytic fields	1170:1223	Owing to their biocompatibility, biodegradability, and controllable properties, wood mimetic hydrogel beads can be used to immobilize various enzymes for applications in the biomedical, bioelectronic, and biocatalytic fields.
25439889	0	19	theme	Wood	0:3	arg1	hydrogel					13:20	Wood mimetic hydrogel	0:20	Wood mimetic hydrogel	0:20	Wood mimetic hydrogel beads for enzyme immobilization.
25439889	4	20	theme	cellulose/xylan/lignin	556:577	arg1	beads					579:583	cellulose/xylan/lignin beads	556:583	cellulose/xylan/lignin beads in a 5:3:2 ratio	556:600	Lipase entrapped in cellulose/xylan/lignin beads in a 5:3:2 ratio showed the highest activity; this ratio is very similar to that in natural wood.
25439889	7	21	theme	hydrogel	1093:1100	arg1	beads					1102:1106	wood mimetic hydrogel beads	1080:1106	wood mimetic hydrogel beads	1080:1106	Owing to their biocompatibility, biodegradability, and controllable properties, wood mimetic hydrogel beads can be used to immobilize various enzymes for applications in the biomedical, bioelectronic, and biocatalytic fields.
25439889	3	22	contain	containing	411:420	arg1	beads					405:409	wood mimetic beads	392:409	wood mimetic beads containing cellulose, xylan, and lignin	392:449	Here, lipase from Candida rugosa was entrapped in wood mimetic beads containing cellulose, xylan, and lignin by dissolving wood components with lipase in [Emim][Ac], followed by reconstitution.
25439889	3	22	contain	containing	411:420	arg2	xylan					433:437	xylan	433:437	xylan	433:437	Here, lipase from Candida rugosa was entrapped in wood mimetic beads containing cellulose, xylan, and lignin by dissolving wood components with lipase in [Emim][Ac], followed by reconstitution.
25439889	3	22	contain	containing	411:420	arg2	cellulose					422:430	cellulose	422:430	cellulose	422:430	Here, lipase from Candida rugosa was entrapped in wood mimetic beads containing cellulose, xylan, and lignin by dissolving wood components with lipase in [Emim][Ac], followed by reconstitution.
25439889	3	22	contain	containing	411:420	arg2	lignin					444:449	lignin	444:449	lignin	444:449	Here, lipase from Candida rugosa was entrapped in wood mimetic beads containing cellulose, xylan, and lignin by dissolving wood components with lipase in [Emim][Ac], followed by reconstitution.
25439889	1	23	theme	potential	101:109	arg1	applications					111:122	potential applications	101:122	potential applications	101:122	Wood component-based composite hydrogels have potential applications in biomedical fields owing to their low cost, biodegradability, and biocompatibility.
25439889	3	24	theme	wood	392:395	arg1	beads					405:409	wood mimetic beads	392:409	wood mimetic beads containing cellulose, xylan, and lignin	392:449	Here, lipase from Candida rugosa was entrapped in wood mimetic beads containing cellulose, xylan, and lignin by dissolving wood components with lipase in [Emim][Ac], followed by reconstitution.
25439889	3	25	theme	mimetic	397:403	arg1	beads					405:409	wood mimetic beads	392:409	wood mimetic beads containing cellulose, xylan, and lignin	392:449	Here, lipase from Candida rugosa was entrapped in wood mimetic beads containing cellulose, xylan, and lignin by dissolving wood components with lipase in [Emim][Ac], followed by reconstitution.
25439889	6	26	theme	temperature	962:972	arg1	conditions					943:952	denaturing conditions	932:952	denaturing conditions of high temperature and low pH	932:983	The half-life times of lipase entrapped in cellulose/alkali lignin hydrogel were 31- and 82-times higher than those of free lipase during incubation under denaturing conditions of high temperature and low pH, respectively.
25439889	7	27	theme	wood	1080:1083	arg1	beads					1102:1106	wood mimetic hydrogel beads	1080:1106	wood mimetic hydrogel beads	1080:1106	Owing to their biocompatibility, biodegradability, and controllable properties, wood mimetic hydrogel beads can be used to immobilize various enzymes for applications in the biomedical, bioelectronic, and biocatalytic fields.
25439889	3	28	from	rugosa	368:373	arg1	lipase					348:353	lipase	348:353	lipase from Candida rugosa	348:373	Here, lipase from Candida rugosa was entrapped in wood mimetic beads containing cellulose, xylan, and lignin by dissolving wood components with lipase in [Emim][Ac], followed by reconstitution.
25439889	2	29	theme	enzyme	319:324	arg1	immobilization					326:339	enzyme immobilization	319:339	enzyme immobilization	319:339	The controllable properties of wood mimetic composites containing three major wood components are useful for enzyme immobilization.
25439889	0	30	theme	enzyme	32:37	arg1	immobilization					39:52	enzyme immobilization	32:52	enzyme immobilization	32:52	Wood mimetic hydrogel beads for enzyme immobilization.
25439889	6	31	theme	high	957:960	arg1	temperature					962:972	high temperature	957:972	high temperature	957:972	The half-life times of lipase entrapped in cellulose/alkali lignin hydrogel were 31- and 82-times higher than those of free lipase during incubation under denaturing conditions of high temperature and low pH, respectively.
25439889	7	32	theme	controllable	1055:1066	arg1	properties					1068:1077	their biocompatibility, biodegradability, and controllable properties	1009:1077	their biocompatibility, biodegradability, and controllable properties	1009:1077	Owing to their biocompatibility, biodegradability, and controllable properties, wood mimetic hydrogel beads can be used to immobilize various enzymes for applications in the biomedical, bioelectronic, and biocatalytic fields.
25439889	2	33	theme	composites	254:263	arg1	useful					308:313	useful	308:313	useful	308:313	The controllable properties of wood mimetic composites containing three major wood components are useful for enzyme immobilization.
25439889	2	33	theme	composites	254:263	arg1	properties					227:236	The controllable properties	210:236	The controllable properties of wood mimetic composites containing three major wood components	210:302	The controllable properties of wood mimetic composites containing three major wood components are useful for enzyme immobilization.
25439889	3	34	theme	wood	465:468	arg1	components					470:479	wood components	465:479	wood components	465:479	Here, lipase from Candida rugosa was entrapped in wood mimetic beads containing cellulose, xylan, and lignin by dissolving wood components with lipase in [Emim][Ac], followed by reconstitution.
25439889	5	35	theme	various	707:713	arg1	beads					728:732	various wood mimetic beads	707:732	various wood mimetic beads	707:732	The lipase entrapped in various wood mimetic beads showed increased thermal and pH stability.
25439889	2	36	theme	mimetic	246:252	arg1	composites					254:263	wood mimetic composites	241:263	wood mimetic composites containing three major wood components	241:302	The controllable properties of wood mimetic composites containing three major wood components are useful for enzyme immobilization.
25439889	7	37	theme	various	1134:1140	arg1	enzymes					1142:1148	various enzymes	1134:1148	various enzymes for applications	1134:1165	Owing to their biocompatibility, biodegradability, and controllable properties, wood mimetic hydrogel beads can be used to immobilize various enzymes for applications in the biomedical, bioelectronic, and biocatalytic fields.
25439889	5	38	theme	thermal	751:757	arg1	stability					766:774	thermal and pH stability	751:774	stability	766:774	The lipase entrapped in various wood mimetic beads showed increased thermal and pH stability.
25439889	2	39	theme	wood	241:244	arg1	composites					254:263	wood mimetic composites	241:263	wood mimetic composites containing three major wood components	241:302	The controllable properties of wood mimetic composites containing three major wood components are useful for enzyme immobilization.
25439889	7	40	theme	biocompatibility	1015:1030	arg1	properties					1068:1077	their biocompatibility, biodegradability, and controllable properties	1009:1077	their biocompatibility, biodegradability, and controllable properties	1009:1077	Owing to their biocompatibility, biodegradability, and controllable properties, wood mimetic hydrogel beads can be used to immobilize various enzymes for applications in the biomedical, bioelectronic, and biocatalytic fields.
25439889	1	41	theme	biomedical	127:136	arg1	fields					138:143	biomedical fields	127:143	biomedical fields owing to their low cost, biodegradability, and biocompatibility	127:207	Wood component-based composite hydrogels have potential applications in biomedical fields owing to their low cost, biodegradability, and biocompatibility.
25439889	6	42	theme	denaturing	932:941	arg1	conditions					943:952	denaturing conditions	932:952	denaturing conditions of high temperature and low pH	932:983	The half-life times of lipase entrapped in cellulose/alkali lignin hydrogel were 31- and 82-times higher than those of free lipase during incubation under denaturing conditions of high temperature and low pH, respectively.
25439889	5	43	dep	showed	734:739	arg1	increased					741:749	increased	741:749	showed increased thermal and pH stability	734:774	The lipase entrapped in various wood mimetic beads showed increased thermal and pH stability.
25439889	6	44	theme	lignin	837:842	arg1	hydrogel					844:851	cellulose/alkali lignin hydrogel	820:851	cellulose/alkali lignin hydrogel	820:851	The half-life times of lipase entrapped in cellulose/alkali lignin hydrogel were 31- and 82-times higher than those of free lipase during incubation under denaturing conditions of high temperature and low pH, respectively.
25439889	1	45	contain	have	96:99	arg1	hydrogels					86:94	Wood component-based composite hydrogels	55:94	Wood component-based composite hydrogels	55:94	Wood component-based composite hydrogels have potential applications in biomedical fields owing to their low cost, biodegradability, and biocompatibility.
25439889	1	45	contain	have	96:99	arg2	applications					111:122	potential applications	101:122	potential applications	101:122	Wood component-based composite hydrogels have potential applications in biomedical fields owing to their low cost, biodegradability, and biocompatibility.
25439889	2	46	theme	controllable	214:225	arg1	useful					308:313	useful	308:313	useful	308:313	The controllable properties of wood mimetic composites containing three major wood components are useful for enzyme immobilization.
25439889	2	46	theme	controllable	214:225	arg1	properties					227:236	The controllable properties	210:236	The controllable properties of wood mimetic composites containing three major wood components	210:302	The controllable properties of wood mimetic composites containing three major wood components are useful for enzyme immobilization.
25439889	2	47	theme	wood	288:291	arg1	components					293:302	three major wood components	276:302	three major wood components	276:302	The controllable properties of wood mimetic composites containing three major wood components are useful for enzyme immobilization.
25439889	4	48	theme	highest	613:619	arg1	activity					621:628	the highest activity	609:628	the highest activity	609:628	Lipase entrapped in cellulose/xylan/lignin beads in a 5:3:2 ratio showed the highest activity; this ratio is very similar to that in natural wood.
25439889	7	49	theme	biodegradability	1033:1048	arg1	properties					1068:1077	their biocompatibility, biodegradability, and controllable properties	1009:1077	their biocompatibility, biodegradability, and controllable properties	1009:1077	Owing to their biocompatibility, biodegradability, and controllable properties, wood mimetic hydrogel beads can be used to immobilize various enzymes for applications in the biomedical, bioelectronic, and biocatalytic fields.
25439889	6	50	theme	cellulose/alkali	820:835	arg1	hydrogel					844:851	cellulose/alkali lignin hydrogel	820:851	cellulose/alkali lignin hydrogel	820:851	The half-life times of lipase entrapped in cellulose/alkali lignin hydrogel were 31- and 82-times higher than those of free lipase during incubation under denaturing conditions of high temperature and low pH, respectively.
25439889	2	51	theme	major	282:286	arg1	components					293:302	three major wood components	276:302	three major wood components	276:302	The controllable properties of wood mimetic composites containing three major wood components are useful for enzyme immobilization.
25439889	4	52	theme	natural	669:675	arg1	wood					677:680	natural wood	669:680	natural wood	669:680	Lipase entrapped in cellulose/xylan/lignin beads in a 5:3:2 ratio showed the highest activity; this ratio is very similar to that in natural wood.
25439889	7	53	used	used	1115:1118	arg2	beads					1102:1106	wood mimetic hydrogel beads	1080:1106	wood mimetic hydrogel beads	1080:1106	Owing to their biocompatibility, biodegradability, and controllable properties, wood mimetic hydrogel beads can be used to immobilize various enzymes for applications in the biomedical, bioelectronic, and biocatalytic fields.
25439889	3	54	theme	Candida	360:366	arg1	rugosa					368:373	Candida rugosa	360:373	Candida rugosa	360:373	Here, lipase from Candida rugosa was entrapped in wood mimetic beads containing cellulose, xylan, and lignin by dissolving wood components with lipase in [Emim][Ac], followed by reconstitution.
25439889	1	55	theme	low	160:162	arg1	cost					164:167	their low cost	154:167	their low cost	154:167	Wood component-based composite hydrogels have potential applications in biomedical fields owing to their low cost, biodegradability, and biocompatibility.
25439889	7	56	theme	biocatalytic	1205:1216	arg1	fields					1218:1223	the biomedical, bioelectronic, and biocatalytic fields	1170:1223	the biomedical, bioelectronic, and biocatalytic fields	1170:1223	Owing to their biocompatibility, biodegradability, and controllable properties, wood mimetic hydrogel beads can be used to immobilize various enzymes for applications in the biomedical, bioelectronic, and biocatalytic fields.
24528759	10	0	theme	loss	1021:1024	arg1	results					1003:1009	The results	999:1009	The results of weight loss, elongation at break and thermal stability	999:1067	The results of weight loss, elongation at break and thermal stability suggested that these novel BC/HA films could be applied potentially as wound dressing materials.
24528759	7	1	theme	films	700:704	arg1	area					670:673	The total surface area	652:673	The total surface area	652:673	The total surface area and pore volume of BC/HA films gradually decreased with the HA content, as followed by FE-SEM analysis.
24528759	7	1	theme	films	700:704	arg1	volume					684:689	pore volume	679:689	pore volume	679:689	The total surface area and pore volume of BC/HA films gradually decreased with the HA content, as followed by FE-SEM analysis.
24528759	10	2	theme	weight	1014:1019	arg1	loss					1021:1024	weight loss	1014:1024	weight loss	1014:1024	The results of weight loss, elongation at break and thermal stability suggested that these novel BC/HA films could be applied potentially as wound dressing materials.
24528759	6	3	theme	water	623:627	arg1	loss					629:632	a lower water loss	615:632	a lower water loss	615:632	Weight loss analysis showed that BC/HA have a lower water loss than BC at 37 °C.
24528759	7	4	theme	pore	679:682	arg1	volume					684:689	pore volume	679:689	pore volume	679:689	The total surface area and pore volume of BC/HA films gradually decreased with the HA content, as followed by FE-SEM analysis.
24528759	9	5	theme	weight	910:915	arg1	loss					917:920	the weight loss	906:920	the weight loss for the BC/HA composites	906:945	Thermogravimetric analysis showed that the weight loss for the BC/HA composites were lower than for pure BC between 250 and 350 °C.
24528759	9	5	theme	weight	910:915	arg1	lower					952:956	lower	952:956	lower	952:956	Thermogravimetric analysis showed that the weight loss for the BC/HA composites were lower than for pure BC between 250 and 350 °C.
24528759	8	6	from	break	797:801	arg1	elongation					783:792	The elongation	779:792	The elongation at break of BC/HA films	779:816	The elongation at break of BC/HA films gradually increased as the HA content increased.
24528759	6	7	theme	lower	617:621	arg1	loss					629:632	a lower water loss	615:632	a lower water loss	615:632	Weight loss analysis showed that BC/HA have a lower water loss than BC at 37 °C.
24528759	10	8	theme	wound	1140:1144	arg1	films					1102:1106	these novel BC/HA films	1084:1106	these novel BC/HA films	1084:1106	The results of weight loss, elongation at break and thermal stability suggested that these novel BC/HA films could be applied potentially as wound dressing materials.
24528759	10	8	theme	wound	1140:1144	arg1	materials					1155:1163	wound dressing materials	1140:1163	wound dressing materials	1140:1163	The results of weight loss, elongation at break and thermal stability suggested that these novel BC/HA films could be applied potentially as wound dressing materials.
24528759	3	9	theme	nanocomposites	365:378	arg1	characterization					320:335	characterization	320:335	characterization	320:335	Here we describe the preparation and characterization of novel BC/hyaluronan (HA) nanocomposites with a 3-D network structure.
24528759	3	9	theme	nanocomposites	365:378	arg1	preparation					304:314	preparation	304:314	preparation	304:314	Here we describe the preparation and characterization of novel BC/hyaluronan (HA) nanocomposites with a 3-D network structure.
24528759	7	10	theme	BC/HA	694:698	arg1	films					700:704	BC/HA films	694:704	BC/HA films	694:704	The total surface area and pore volume of BC/HA films gradually decreased with the HA content, as followed by FE-SEM analysis.
24528759	3	11	dep	preparation	304:314	arg1	the					300:302	the	300:302	the	300:302	Here we describe the preparation and characterization of novel BC/hyaluronan (HA) nanocomposites with a 3-D network structure.
24528759	10	12	theme	break	1041:1045	arg1	results					1003:1009	The results	999:1009	The results of weight loss, elongation at break and thermal stability	999:1067	The results of weight loss, elongation at break and thermal stability suggested that these novel BC/HA films could be applied potentially as wound dressing materials.
24528759	10	13	from	break	1041:1045	arg1	at					1038:1039	elongation at break	1027:1045	elongation at break	1027:1045	The results of weight loss, elongation at break and thermal stability suggested that these novel BC/HA films could be applied potentially as wound dressing materials.
24528759	10	13	from	break	1041:1045	arg1	elongation					1027:1036	elongation at break	1027:1045	elongation at break	1027:1045	The results of weight loss, elongation at break and thermal stability suggested that these novel BC/HA films could be applied potentially as wound dressing materials.
24528759	10	14	theme	at	1038:1039	arg1	break					1041:1045	elongation at break	1027:1045	elongation at break	1027:1045	The results of weight loss, elongation at break and thermal stability suggested that these novel BC/HA films could be applied potentially as wound dressing materials.
24528759	8	15	theme	HA	845:846	arg1	content					848:854	the HA content	841:854	the HA content	841:854	The elongation at break of BC/HA films gradually increased as the HA content increased.
24528759	3	16	theme	3-D	387:389	arg1	structure					399:407	a 3-D network structure	385:407	a 3-D network structure	385:407	Here we describe the preparation and characterization of novel BC/hyaluronan (HA) nanocomposites with a 3-D network structure.
24528759	10	17	theme	elongation	1027:1036	arg1	break					1041:1045	elongation at break	1027:1045	elongation at break	1027:1045	The results of weight loss, elongation at break and thermal stability suggested that these novel BC/HA films could be applied potentially as wound dressing materials.
24528759	3	18	theme	network	391:397	arg1	structure					399:407	a 3-D network structure	385:407	a 3-D network structure	385:407	Here we describe the preparation and characterization of novel BC/hyaluronan (HA) nanocomposites with a 3-D network structure.
24528759	10	19	theme	thermal	1051:1057	arg1	stability					1059:1067	thermal stability	1051:1067	thermal stability	1051:1067	The results of weight loss, elongation at break and thermal stability suggested that these novel BC/HA films could be applied potentially as wound dressing materials.
24528759	0	20	theme	cellulose/hyaluronan	24:43	arg1	biomaterials					59:70	bacterial cellulose/hyaluronan nanocomposite biomaterials	14:70	bacterial cellulose/hyaluronan nanocomposite biomaterials	14:70	Evaluation of bacterial cellulose/hyaluronan nanocomposite biomaterials.
24528759	1	21	from	field	126:130	arg1	useful					101:106	useful	101:106	useful	101:106	Bacterial cellulose (BC) is useful in the biomedical field because of its unique structure and properties.
24528759	5	22	theme	ATR-FTIR	481:488	arg1	analyses					490:497	Elemental and ATR-FTIR analyses	467:497	Elemental and ATR-FTIR analyses	467:497	Elemental and ATR-FTIR analyses showed that this method is highly effective to form composites with BC.
24528759	0	23	theme	bacterial	14:22	arg1	biomaterials					59:70	bacterial cellulose/hyaluronan nanocomposite biomaterials	14:70	bacterial cellulose/hyaluronan nanocomposite biomaterials	14:70	Evaluation of bacterial cellulose/hyaluronan nanocomposite biomaterials.
24528759	10	24	theme	stability	1059:1067	arg1	results					1003:1009	The results	999:1009	The results of weight loss, elongation at break and thermal stability	999:1067	The results of weight loss, elongation at break and thermal stability suggested that these novel BC/HA films could be applied potentially as wound dressing materials.
24528759	2	25	theme	high	184:187	arg1	nano-porosity					189:201	The high nano-porosity	180:201	The high nano-porosity of BC	180:207	The high nano-porosity of BC allows other materials to be incorporated and form reinforced composites.
24528759	0	26	theme	biomaterials	59:70	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of bacterial cellulose/hyaluronan nanocomposite biomaterials.	0:71	Evaluation of bacterial cellulose/hyaluronan nanocomposite biomaterials.
24528759	7	27	theme	FE-SEM	762:767	arg1	analysis					769:776	FE-SEM analysis	762:776	FE-SEM analysis	762:776	The total surface area and pore volume of BC/HA films gradually decreased with the HA content, as followed by FE-SEM analysis.
24528759	4	28	theme	impregnation	446:457	arg1	method					459:464	a solution impregnation method	435:464	a solution impregnation method	435:464	BC/HA was obtained using a solution impregnation method.
24528759	5	29	theme	Elemental	467:475	arg1	analyses					490:497	Elemental and ATR-FTIR analyses	467:497	Elemental and ATR-FTIR analyses	467:497	Elemental and ATR-FTIR analyses showed that this method is highly effective to form composites with BC.
24528759	0	30	theme	nanocomposite	45:57	arg1	biomaterials					59:70	bacterial cellulose/hyaluronan nanocomposite biomaterials	14:70	bacterial cellulose/hyaluronan nanocomposite biomaterials	14:70	Evaluation of bacterial cellulose/hyaluronan nanocomposite biomaterials.
24528759	10	31	theme	dressing	1146:1153	arg1	films					1102:1106	these novel BC/HA films	1084:1106	these novel BC/HA films	1084:1106	The results of weight loss, elongation at break and thermal stability suggested that these novel BC/HA films could be applied potentially as wound dressing materials.
24528759	10	31	theme	dressing	1146:1153	arg1	materials					1155:1163	wound dressing materials	1140:1163	wound dressing materials	1140:1163	The results of weight loss, elongation at break and thermal stability suggested that these novel BC/HA films could be applied potentially as wound dressing materials.
24528759	3	32	with	nanocomposites	365:378	arg1	structure					399:407	a 3-D network structure	385:407	a 3-D network structure	385:407	Here we describe the preparation and characterization of novel BC/hyaluronan (HA) nanocomposites with a 3-D network structure.
24528759	6	33	theme	loss	578:581	arg1	analysis					583:590	Weight loss analysis	571:590	Weight loss analysis	571:590	Weight loss analysis showed that BC/HA have a lower water loss than BC at 37 °C.
24528759	10	34	theme	BC/HA	1096:1100	arg1	materials					1155:1163	wound dressing materials	1140:1163	wound dressing materials	1140:1163	The results of weight loss, elongation at break and thermal stability suggested that these novel BC/HA films could be applied potentially as wound dressing materials.
24528759	10	34	theme	BC/HA	1096:1100	arg1	films					1102:1106	these novel BC/HA films	1084:1106	these novel BC/HA films	1084:1106	The results of weight loss, elongation at break and thermal stability suggested that these novel BC/HA films could be applied potentially as wound dressing materials.
24528759	1	35	theme	biomedical	115:124	arg1	field					126:130	the biomedical field	111:130	the biomedical field	111:130	Bacterial cellulose (BC) is useful in the biomedical field because of its unique structure and properties.
24528759	6	36	theme	Weight	571:576	arg1	loss					578:581	Weight loss	571:581	Weight loss analysis	571:590	Weight loss analysis showed that BC/HA have a lower water loss than BC at 37 °C.
24528759	10	37	theme	novel	1090:1094	arg1	materials					1155:1163	wound dressing materials	1140:1163	wound dressing materials	1140:1163	The results of weight loss, elongation at break and thermal stability suggested that these novel BC/HA films could be applied potentially as wound dressing materials.
24528759	10	37	theme	novel	1090:1094	arg1	films					1102:1106	these novel BC/HA films	1084:1106	these novel BC/HA films	1084:1106	The results of weight loss, elongation at break and thermal stability suggested that these novel BC/HA films could be applied potentially as wound dressing materials.
24528759	7	38	theme	total	656:660	arg1	area					670:673	The total surface area	652:673	The total surface area	652:673	The total surface area and pore volume of BC/HA films gradually decreased with the HA content, as followed by FE-SEM analysis.
24528759	2	39	theme	other	216:220	arg1	materials					222:230	other materials	216:230	other materials	216:230	The high nano-porosity of BC allows other materials to be incorporated and form reinforced composites.
24528759	6	40	contain	have	610:613	arg1	BC/HA					604:608	BC/HA	604:608	BC/HA	604:608	Weight loss analysis showed that BC/HA have a lower water loss than BC at 37 °C.
24528759	6	40	contain	have	610:613	arg2	loss					629:632	a lower water loss	615:632	a lower water loss	615:632	Weight loss analysis showed that BC/HA have a lower water loss than BC at 37 °C.
24528759	2	41	theme	BC	206:207	arg1	nano-porosity					189:201	The high nano-porosity	180:201	The high nano-porosity of BC	180:207	The high nano-porosity of BC allows other materials to be incorporated and form reinforced composites.
24528759	2	42	theme	reinforced	260:269	arg1	composites					271:280	reinforced composites	260:280	reinforced composites	260:280	The high nano-porosity of BC allows other materials to be incorporated and form reinforced composites.
24528759	7	43	theme	surface	662:668	arg1	area					670:673	The total surface area	652:673	The total surface area	652:673	The total surface area and pore volume of BC/HA films gradually decreased with the HA content, as followed by FE-SEM analysis.
24528759	9	44	theme	Thermogravimetric	867:883	arg1	analysis					885:892	Thermogravimetric analysis	867:892	Thermogravimetric analysis	867:892	Thermogravimetric analysis showed that the weight loss for the BC/HA composites were lower than for pure BC between 250 and 350 °C.
24528759	4	45	theme	solution	437:444	arg1	method					459:464	a solution impregnation method	435:464	a solution impregnation method	435:464	BC/HA was obtained using a solution impregnation method.
24528759	7	46	theme	HA	735:736	arg1	content					738:744	the HA content	731:744	the HA content	731:744	The total surface area and pore volume of BC/HA films gradually decreased with the HA content, as followed by FE-SEM analysis.
24528759	3	47	theme	novel	340:344	arg1	HA					361:362	HA	361:362	HA	361:362	Here we describe the preparation and characterization of novel BC/hyaluronan (HA) nanocomposites with a 3-D network structure.
24528759	3	47	theme	novel	340:344	arg1	BC/hyaluronan					346:358	novel BC/hyaluronan	340:358	novel BC/hyaluronan (HA) nanocomposites with a 3-D network structure	340:407	Here we describe the preparation and characterization of novel BC/hyaluronan (HA) nanocomposites with a 3-D network structure.
24528759	8	48	theme	films	812:816	arg1	elongation					783:792	The elongation	779:792	The elongation at break of BC/HA films	779:816	The elongation at break of BC/HA films gradually increased as the HA content increased.
24528759	9	49	theme	BC/HA	930:934	arg1	composites					936:945	the BC/HA composites	926:945	the BC/HA composites	926:945	Thermogravimetric analysis showed that the weight loss for the BC/HA composites were lower than for pure BC between 250 and 350 °C.
24528759	1	50	from	useful	101:106	arg1	field					126:130	the biomedical field	111:130	the biomedical field	111:130	Bacterial cellulose (BC) is useful in the biomedical field because of its unique structure and properties.
24528759	9	51	theme	pure	967:970	arg1	BC					972:973	pure BC	967:973	pure BC between 250 and 350 °C	967:996	Thermogravimetric analysis showed that the weight loss for the BC/HA composites were lower than for pure BC between 250 and 350 °C.
24528759	3	52	theme	BC/hyaluronan	346:358	arg1	nanocomposites					365:378	novel BC/hyaluronan (HA) nanocomposites	340:378	novel BC/hyaluronan (HA) nanocomposites with a 3-D network structure	340:407	Here we describe the preparation and characterization of novel BC/hyaluronan (HA) nanocomposites with a 3-D network structure.
24528759	1	53	theme	Bacterial	73:81	arg1	BC					94:95	BC	94:95	BC	94:95	Bacterial cellulose (BC) is useful in the biomedical field because of its unique structure and properties.
24528759	1	53	theme	Bacterial	73:81	arg1	cellulose					83:91	Bacterial cellulose	73:91	Bacterial cellulose (BC)	73:96	Bacterial cellulose (BC) is useful in the biomedical field because of its unique structure and properties.
24528759	1	54	theme	unique	147:152	arg1	structure					154:162	its unique structure	143:162	its unique structure	143:162	Bacterial cellulose (BC) is useful in the biomedical field because of its unique structure and properties.
24528759	8	55	theme	BC/HA	806:810	arg1	films					812:816	BC/HA films	806:816	BC/HA films	806:816	The elongation at break of BC/HA films gradually increased as the HA content increased.
25825076	9	0	theme	release	1177:1183	arg1	mechanism					1185:1193	The drug release mechanism	1168:1193	The drug release mechanism	1168:1193	The drug release mechanism deviated from non-Fickian to case-II type with increasing resin concentration in the composites.
25825076	6	1	theme	drug	891:894	arg1	faster					935:940	faster	935:940	faster	935:940	The drug release rate of polymer composites was faster in phosphate buffer solution (pH 6.8).
25825076	6	1	theme	drug	891:894	arg1	rate					904:907	The drug release rate	887:907	The drug release rate of polymer composites	887:929	The drug release rate of polymer composites was faster in phosphate buffer solution (pH 6.8).
25825076	9	2	dep	type	1232:1235	arg1	non-Fickian					1209:1219	non-Fickian	1209:1219	non-Fickian	1209:1219	The drug release mechanism deviated from non-Fickian to case-II type with increasing resin concentration in the composites.
25825076	8	3	theme	drug	1052:1055	arg1	rate					1065:1068	the drug release rate	1048:1068	the drug release rate	1048:1068	In same duration, the drug release rate suddenly boosted up to 92% as the concentration of gum resin in the composites was raised to 80%.
25825076	1	4	theme	gum	157:159	arg1	resin					161:165	Boswellia gum resin	147:165	Boswellia gum resin	147:165	This study was undertaken to evaluate the effect of Boswellia gum resin on the properties of glutaraldehyde (GA) crosslinked chitosan polymer composites and their potential as oral delivery vehicles for a non-steroidal anti-inflammatory drug, aceclofenac.
25825076	8	5	from	concentration	1104:1116	arg1	composites					1138:1147	the composites	1134:1147	the composites	1134:1147	In same duration, the drug release rate suddenly boosted up to 92% as the concentration of gum resin in the composites was raised to 80%.
25825076	5	6	dep	%	833:833	arg1	8-17					829:832	8-17	829:832	8-17	829:832	Only 8-17% drug was released into HCl solution (pH 1.2) in 2h.
25825076	10	7	theme	Boswellia	1310:1318	arg1	composites					1335:1344	GA-treated Boswellia resin-chitosan composites	1299:1344	GA-treated Boswellia resin-chitosan composites	1299:1344	Hence, GA-treated Boswellia resin-chitosan composites could be considered as alternative vehicles for oral delivery of aceclofenac.
25825076	10	7	theme	Boswellia	1310:1318	arg1	vehicles					1381:1388	alternative vehicles	1369:1388	alternative vehicles for oral delivery of aceclofenac	1369:1421	Hence, GA-treated Boswellia resin-chitosan composites could be considered as alternative vehicles for oral delivery of aceclofenac.
25825076	1	8	theme	resin	161:165	arg1	effect					137:142	the effect	133:142	the effect of Boswellia gum resin on the properties of glutaraldehyde (GA) crosslinked chitosan polymer composites and their potential as oral delivery vehicles for a non-steroidal anti-inflammatory drug, aceclofenac	133:348	This study was undertaken to evaluate the effect of Boswellia gum resin on the properties of glutaraldehyde (GA) crosslinked chitosan polymer composites and their potential as oral delivery vehicles for a non-steroidal anti-inflammatory drug, aceclofenac.
25825076	10	9	theme	alternative	1369:1379	arg1	composites					1335:1344	GA-treated Boswellia resin-chitosan composites	1299:1344	GA-treated Boswellia resin-chitosan composites	1299:1344	Hence, GA-treated Boswellia resin-chitosan composites could be considered as alternative vehicles for oral delivery of aceclofenac.
25825076	10	9	theme	alternative	1369:1379	arg1	vehicles					1381:1388	alternative vehicles	1369:1388	alternative vehicles for oral delivery of aceclofenac	1369:1421	Hence, GA-treated Boswellia resin-chitosan composites could be considered as alternative vehicles for oral delivery of aceclofenac.
25825076	4	10	theme	mass	805:808	arg1	ratio					810:814	gum resin mass ratio	795:814	particulate polymer composites up to chitosan:gum resin mass ratio of 1:3	749:821	Field emission scanning electron microscopy (FE-SEM) suggested the formation of particulate polymer composites up to chitosan:gum resin mass ratio of 1:3.
25825076	8	11	dep	92	1093:1094	arg1	to					1090:1091	to	1090:1091	to	1090:1091	In same duration, the drug release rate suddenly boosted up to 92% as the concentration of gum resin in the composites was raised to 80%.
25825076	10	12	theme	GA-treated	1299:1308	arg1	composites					1335:1344	GA-treated Boswellia resin-chitosan composites	1299:1344	GA-treated Boswellia resin-chitosan composites	1299:1344	Hence, GA-treated Boswellia resin-chitosan composites could be considered as alternative vehicles for oral delivery of aceclofenac.
25825076	10	12	theme	GA-treated	1299:1308	arg1	vehicles					1381:1388	alternative vehicles	1369:1388	alternative vehicles for oral delivery of aceclofenac	1369:1421	Hence, GA-treated Boswellia resin-chitosan composites could be considered as alternative vehicles for oral delivery of aceclofenac.
25825076	4	13	theme	composites	769:778	arg1	formation					736:744	the formation	732:744	the formation of particulate polymer composites up to chitosan:gum resin mass ratio of 1:3	732:821	Field emission scanning electron microscopy (FE-SEM) suggested the formation of particulate polymer composites up to chitosan:gum resin mass ratio of 1:3.
25825076	5	14	theme	HCl	858:860	arg1	solution					862:869	HCl solution	858:869	HCl solution (pH 1.2)	858:878	Only 8-17% drug was released into HCl solution (pH 1.2) in 2h.
25825076	5	14	theme	HCl	858:860	arg1	pH					872:873	pH 1.2	872:877	pH 1.2	872:877	Only 8-17% drug was released into HCl solution (pH 1.2) in 2h.
25825076	4	15	theme	polymer	761:767	arg1	composites					769:778	particulate polymer composites	749:778	particulate polymer composites up to chitosan:gum resin mass ratio of 1:3	749:821	Field emission scanning electron microscopy (FE-SEM) suggested the formation of particulate polymer composites up to chitosan:gum resin mass ratio of 1:3.
25825076	4	16	theme	resin	799:803	arg1	ratio					810:814	gum resin mass ratio	795:814	particulate polymer composites up to chitosan:gum resin mass ratio of 1:3	749:821	Field emission scanning electron microscopy (FE-SEM) suggested the formation of particulate polymer composites up to chitosan:gum resin mass ratio of 1:3.
25825076	4	17	theme	scanning	684:691	arg1	FE-SEM					714:719	FE-SEM	714:719	FE-SEM	714:719	Field emission scanning electron microscopy (FE-SEM) suggested the formation of particulate polymer composites up to chitosan:gum resin mass ratio of 1:3.
25825076	4	17	theme	scanning	684:691	arg1	microscopy					702:711	Field emission scanning electron microscopy	669:711	Field emission scanning electron microscopy (FE-SEM)	669:720	Field emission scanning electron microscopy (FE-SEM) suggested the formation of particulate polymer composites up to chitosan:gum resin mass ratio of 1:3.
25825076	6	18	theme	buffer	955:960	arg1	pH					972:973	pH 6.8	972:977	pH 6.8	972:977	The drug release rate of polymer composites was faster in phosphate buffer solution (pH 6.8).
25825076	6	18	theme	buffer	955:960	arg1	solution					962:969	phosphate buffer solution	945:969	phosphate buffer solution (pH 6.8)	945:978	The drug release rate of polymer composites was faster in phosphate buffer solution (pH 6.8).
25825076	9	19	theme	case-II	1224:1230	arg1	type					1232:1235	case-II type	1224:1235	case-II type	1224:1235	The drug release mechanism deviated from non-Fickian to case-II type with increasing resin concentration in the composites.
25825076	10	20	theme	aceclofenac	1411:1421	arg1	delivery					1399:1406	oral delivery	1394:1406	oral delivery of aceclofenac	1394:1421	Hence, GA-treated Boswellia resin-chitosan composites could be considered as alternative vehicles for oral delivery of aceclofenac.
25825076	3	21	dep	infrared	505:512	arg1	FTIR					515:518	FTIR	515:518	FTIR	515:518	Fourier transform infrared (FTIR) spectroscopic analysis confirmed the formation of chitosan-gum resin composites and did not show any evidence of drug-polymer chemical interaction.
25825076	2	22	theme	significant	399:409	arg1	improvement					411:421	a significant improvement	397:421	a significant improvement in drug entrapment efficiency (∼40%) of the polymer composites	397:484	The incorporation of resinous material caused a significant improvement in drug entrapment efficiency (∼40%) of the polymer composites.
25825076	0	23	theme	gum	10:12	arg1	composites					37:46	Boswellia gum resin/chitosan polymer composites	0:46	Boswellia gum resin/chitosan polymer composites: Controlled delivery vehicles for aceclofenac.	0:93	Boswellia gum resin/chitosan polymer composites: Controlled delivery vehicles for aceclofenac.
25825076	3	24	theme	spectroscopic	521:533	arg1	analysis					535:542	spectroscopic analysis	521:542	spectroscopic analysis	521:542	Fourier transform infrared (FTIR) spectroscopic analysis confirmed the formation of chitosan-gum resin composites and did not show any evidence of drug-polymer chemical interaction.
25825076	10	25	theme	oral	1394:1397	arg1	delivery					1399:1406	oral delivery	1394:1406	oral delivery of aceclofenac	1394:1421	Hence, GA-treated Boswellia resin-chitosan composites could be considered as alternative vehicles for oral delivery of aceclofenac.
25825076	1	26	from	effect	137:142	arg1	properties					174:183	the properties	170:183	the properties of glutaraldehyde (GA) crosslinked chitosan polymer composites	170:246	This study was undertaken to evaluate the effect of Boswellia gum resin on the properties of glutaraldehyde (GA) crosslinked chitosan polymer composites and their potential as oral delivery vehicles for a non-steroidal anti-inflammatory drug, aceclofenac.
25825076	1	26	from	effect	137:142	arg1	potential					258:266	their potential	252:266	their potential as oral delivery vehicles for a non-steroidal anti-inflammatory drug, aceclofenac	252:348	This study was undertaken to evaluate the effect of Boswellia gum resin on the properties of glutaraldehyde (GA) crosslinked chitosan polymer composites and their potential as oral delivery vehicles for a non-steroidal anti-inflammatory drug, aceclofenac.
25825076	3	27	theme	resin	584:588	arg1	composites					590:599	chitosan-gum resin composites	571:599	chitosan-gum resin composites	571:599	Fourier transform infrared (FTIR) spectroscopic analysis confirmed the formation of chitosan-gum resin composites and did not show any evidence of drug-polymer chemical interaction.
25825076	0	28	theme	Boswellia	0:8	arg1	composites					37:46	Boswellia gum resin/chitosan polymer composites	0:46	Boswellia gum resin/chitosan polymer composites: Controlled delivery vehicles for aceclofenac.	0:93	Boswellia gum resin/chitosan polymer composites: Controlled delivery vehicles for aceclofenac.
25825076	1	29	theme	oral	271:274	arg1	vehicles					285:292	oral delivery vehicles	271:292	oral delivery vehicles for a non-steroidal anti-inflammatory drug, aceclofenac	271:348	This study was undertaken to evaluate the effect of Boswellia gum resin on the properties of glutaraldehyde (GA) crosslinked chitosan polymer composites and their potential as oral delivery vehicles for a non-steroidal anti-inflammatory drug, aceclofenac.
25825076	4	30	dep	composites	769:778	arg1	ratio					810:814	gum resin mass ratio	795:814	particulate polymer composites up to chitosan:gum resin mass ratio of 1:3	749:821	Field emission scanning electron microscopy (FE-SEM) suggested the formation of particulate polymer composites up to chitosan:gum resin mass ratio of 1:3.
25825076	1	31	link	crosslinked	208:218	arg1	composites					237:246	glutaraldehyde (GA) crosslinked chitosan polymer composites	188:246	glutaraldehyde (GA) crosslinked chitosan polymer composites	188:246	This study was undertaken to evaluate the effect of Boswellia gum resin on the properties of glutaraldehyde (GA) crosslinked chitosan polymer composites and their potential as oral delivery vehicles for a non-steroidal anti-inflammatory drug, aceclofenac.
25825076	3	32	theme	composites	590:599	arg1	formation					558:566	the formation	554:566	the formation of chitosan-gum resin composites	554:599	Fourier transform infrared (FTIR) spectroscopic analysis confirmed the formation of chitosan-gum resin composites and did not show any evidence of drug-polymer chemical interaction.
25825076	8	33	theme	same	1033:1036	arg1	duration					1038:1045	same duration	1033:1045	same duration	1033:1045	In same duration, the drug release rate suddenly boosted up to 92% as the concentration of gum resin in the composites was raised to 80%.
25825076	4	34	theme	electron	693:700	arg1	FE-SEM					714:719	FE-SEM	714:719	FE-SEM	714:719	Field emission scanning electron microscopy (FE-SEM) suggested the formation of particulate polymer composites up to chitosan:gum resin mass ratio of 1:3.
25825076	4	34	theme	electron	693:700	arg1	microscopy					702:711	Field emission scanning electron microscopy	669:711	Field emission scanning electron microscopy (FE-SEM)	669:720	Field emission scanning electron microscopy (FE-SEM) suggested the formation of particulate polymer composites up to chitosan:gum resin mass ratio of 1:3.
25825076	0	35	theme	polymer	29:35	arg1	composites					37:46	Boswellia gum resin/chitosan polymer composites	0:46	Boswellia gum resin/chitosan polymer composites: Controlled delivery vehicles for aceclofenac.	0:93	Boswellia gum resin/chitosan polymer composites: Controlled delivery vehicles for aceclofenac.
25825076	1	36	theme	delivery	276:283	arg1	vehicles					285:292	oral delivery vehicles	271:292	oral delivery vehicles for a non-steroidal anti-inflammatory drug, aceclofenac	271:348	This study was undertaken to evaluate the effect of Boswellia gum resin on the properties of glutaraldehyde (GA) crosslinked chitosan polymer composites and their potential as oral delivery vehicles for a non-steroidal anti-inflammatory drug, aceclofenac.
25825076	10	37	theme	resin-chitosan	1320:1333	arg1	composites					1335:1344	GA-treated Boswellia resin-chitosan composites	1299:1344	GA-treated Boswellia resin-chitosan composites	1299:1344	Hence, GA-treated Boswellia resin-chitosan composites could be considered as alternative vehicles for oral delivery of aceclofenac.
25825076	10	37	theme	resin-chitosan	1320:1333	arg1	vehicles					1381:1388	alternative vehicles	1369:1388	alternative vehicles for oral delivery of aceclofenac	1369:1421	Hence, GA-treated Boswellia resin-chitosan composites could be considered as alternative vehicles for oral delivery of aceclofenac.
25825076	0	38	theme	resin/chitosan	14:27	arg1	composites					37:46	Boswellia gum resin/chitosan polymer composites	0:46	Boswellia gum resin/chitosan polymer composites: Controlled delivery vehicles for aceclofenac.	0:93	Boswellia gum resin/chitosan polymer composites: Controlled delivery vehicles for aceclofenac.
25825076	5	39	dep	drug	835:838	arg1	%					833:833	%	833:833	%	833:833	Only 8-17% drug was released into HCl solution (pH 1.2) in 2h.
25825076	3	40	theme	chitosan-gum	571:582	arg1	composites					590:599	chitosan-gum resin composites	571:599	chitosan-gum resin composites	571:599	Fourier transform infrared (FTIR) spectroscopic analysis confirmed the formation of chitosan-gum resin composites and did not show any evidence of drug-polymer chemical interaction.
25825076	2	41	theme	entrapment	431:440	arg1	%					457:457	∼40%	454:457	∼40%	454:457	The incorporation of resinous material caused a significant improvement in drug entrapment efficiency (∼40%) of the polymer composites.
25825076	2	41	theme	entrapment	431:440	arg1	efficiency					442:451	drug entrapment efficiency	426:451	drug entrapment efficiency (∼40%) of the polymer composites	426:484	The incorporation of resinous material caused a significant improvement in drug entrapment efficiency (∼40%) of the polymer composites.
25825076	2	42	theme	composites	475:484	arg1	%					457:457	∼40%	454:457	∼40%	454:457	The incorporation of resinous material caused a significant improvement in drug entrapment efficiency (∼40%) of the polymer composites.
25825076	2	42	theme	composites	475:484	arg1	efficiency					442:451	drug entrapment efficiency	426:451	drug entrapment efficiency (∼40%) of the polymer composites	426:484	The incorporation of resinous material caused a significant improvement in drug entrapment efficiency (∼40%) of the polymer composites.
25825076	7	43	theme	drug	1013:1016	arg1	load					1018:1021	∼60-68% drug load	1005:1021	∼60-68% drug load	1005:1021	The composites released ∼60-68% drug load in 7h.
25825076	4	44	theme	Field	669:673	arg1	FE-SEM					714:719	FE-SEM	714:719	FE-SEM	714:719	Field emission scanning electron microscopy (FE-SEM) suggested the formation of particulate polymer composites up to chitosan:gum resin mass ratio of 1:3.
25825076	4	44	theme	Field	669:673	arg1	microscopy					702:711	Field emission scanning electron microscopy	669:711	Field emission scanning electron microscopy (FE-SEM)	669:720	Field emission scanning electron microscopy (FE-SEM) suggested the formation of particulate polymer composites up to chitosan:gum resin mass ratio of 1:3.
25825076	2	45	theme	drug	426:429	arg1	%					457:457	∼40%	454:457	∼40%	454:457	The incorporation of resinous material caused a significant improvement in drug entrapment efficiency (∼40%) of the polymer composites.
25825076	2	45	theme	drug	426:429	arg1	efficiency					442:451	drug entrapment efficiency	426:451	drug entrapment efficiency (∼40%) of the polymer composites	426:484	The incorporation of resinous material caused a significant improvement in drug entrapment efficiency (∼40%) of the polymer composites.
25825076	2	46	theme	polymer	467:473	arg1	composites					475:484	the polymer composites	463:484	the polymer composites	463:484	The incorporation of resinous material caused a significant improvement in drug entrapment efficiency (∼40%) of the polymer composites.
25825076	6	47	theme	composites	920:929	arg1	faster					935:940	faster	935:940	faster	935:940	The drug release rate of polymer composites was faster in phosphate buffer solution (pH 6.8).
25825076	6	47	theme	composites	920:929	arg1	rate					904:907	The drug release rate	887:907	The drug release rate of polymer composites	887:929	The drug release rate of polymer composites was faster in phosphate buffer solution (pH 6.8).
25825076	8	48	theme	resin	1125:1129	arg1	concentration					1104:1116	the concentration	1100:1116	the concentration of gum resin in the composites	1100:1147	In same duration, the drug release rate suddenly boosted up to 92% as the concentration of gum resin in the composites was raised to 80%.
25825076	1	49	dep	crosslinked	208:218	arg1	GA					204:205	GA	204:205	GA	204:205	This study was undertaken to evaluate the effect of Boswellia gum resin on the properties of glutaraldehyde (GA) crosslinked chitosan polymer composites and their potential as oral delivery vehicles for a non-steroidal anti-inflammatory drug, aceclofenac.
25825076	1	49	dep	crosslinked	208:218	arg1	glutaraldehyde					188:201	glutaraldehyde	188:201	glutaraldehyde (GA)	188:206	This study was undertaken to evaluate the effect of Boswellia gum resin on the properties of glutaraldehyde (GA) crosslinked chitosan polymer composites and their potential as oral delivery vehicles for a non-steroidal anti-inflammatory drug, aceclofenac.
25825076	8	50	theme	gum	1121:1123	arg1	resin					1125:1129	gum resin	1121:1129	gum resin	1121:1129	In same duration, the drug release rate suddenly boosted up to 92% as the concentration of gum resin in the composites was raised to 80%.
25825076	0	51	theme	delivery	60:67	arg1	vehicles					69:76	Controlled delivery vehicles	49:76	Boswellia gum resin/chitosan polymer composites: Controlled delivery vehicles for aceclofenac.	0:93	Boswellia gum resin/chitosan polymer composites: Controlled delivery vehicles for aceclofenac.
25825076	1	52	theme	non-steroidal	300:312	arg1	aceclofenac					338:348	aceclofenac	338:348	aceclofenac	338:348	This study was undertaken to evaluate the effect of Boswellia gum resin on the properties of glutaraldehyde (GA) crosslinked chitosan polymer composites and their potential as oral delivery vehicles for a non-steroidal anti-inflammatory drug, aceclofenac.
25825076	1	52	theme	non-steroidal	300:312	arg1	drug					332:335	a non-steroidal anti-inflammatory drug	298:335	a non-steroidal anti-inflammatory drug	298:335	This study was undertaken to evaluate the effect of Boswellia gum resin on the properties of glutaraldehyde (GA) crosslinked chitosan polymer composites and their potential as oral delivery vehicles for a non-steroidal anti-inflammatory drug, aceclofenac.
25825076	7	53	theme	∼60-68	1005:1010	arg1	%					1011:1011	%	1011:1011	%	1011:1011	The composites released ∼60-68% drug load in 7h.
25825076	3	54	theme	interaction	656:666	arg1	evidence					622:629	any evidence	618:629	any evidence of drug-polymer chemical interaction	618:666	Fourier transform infrared (FTIR) spectroscopic analysis confirmed the formation of chitosan-gum resin composites and did not show any evidence of drug-polymer chemical interaction.
25825076	8	55	theme	release	1057:1063	arg1	rate					1065:1068	the drug release rate	1048:1068	the drug release rate	1048:1068	In same duration, the drug release rate suddenly boosted up to 92% as the concentration of gum resin in the composites was raised to 80%.
25825076	0	56	theme	Controlled	49:58	arg1	vehicles					69:76	Controlled delivery vehicles	49:76	Boswellia gum resin/chitosan polymer composites: Controlled delivery vehicles for aceclofenac.	0:93	Boswellia gum resin/chitosan polymer composites: Controlled delivery vehicles for aceclofenac.
25825076	1	57	theme	anti-inflammatory	314:330	arg1	aceclofenac					338:348	aceclofenac	338:348	aceclofenac	338:348	This study was undertaken to evaluate the effect of Boswellia gum resin on the properties of glutaraldehyde (GA) crosslinked chitosan polymer composites and their potential as oral delivery vehicles for a non-steroidal anti-inflammatory drug, aceclofenac.
25825076	1	57	theme	anti-inflammatory	314:330	arg1	drug					332:335	a non-steroidal anti-inflammatory drug	298:335	a non-steroidal anti-inflammatory drug	298:335	This study was undertaken to evaluate the effect of Boswellia gum resin on the properties of glutaraldehyde (GA) crosslinked chitosan polymer composites and their potential as oral delivery vehicles for a non-steroidal anti-inflammatory drug, aceclofenac.
25825076	7	58	theme	%	1011:1011	arg1	load					1018:1021	∼60-68% drug load	1005:1021	∼60-68% drug load	1005:1021	The composites released ∼60-68% drug load in 7h.
25825076	0	59	dep	composites	37:46	arg1	vehicles					69:76	Controlled delivery vehicles	49:76	Boswellia gum resin/chitosan polymer composites: Controlled delivery vehicles for aceclofenac.	0:93	Boswellia gum resin/chitosan polymer composites: Controlled delivery vehicles for aceclofenac.
25825076	2	60	from	improvement	411:421	arg1	%					457:457	∼40%	454:457	∼40%	454:457	The incorporation of resinous material caused a significant improvement in drug entrapment efficiency (∼40%) of the polymer composites.
25825076	2	60	from	improvement	411:421	arg1	efficiency					442:451	drug entrapment efficiency	426:451	drug entrapment efficiency (∼40%) of the polymer composites	426:484	The incorporation of resinous material caused a significant improvement in drug entrapment efficiency (∼40%) of the polymer composites.
25825076	3	61	theme	chemical	647:654	arg1	interaction					656:666	drug-polymer chemical interaction	634:666	drug-polymer chemical interaction	634:666	Fourier transform infrared (FTIR) spectroscopic analysis confirmed the formation of chitosan-gum resin composites and did not show any evidence of drug-polymer chemical interaction.
25825076	4	62	dep	chitosan	786:793	arg1	up					780:781	up	780:781	up	780:781	Field emission scanning electron microscopy (FE-SEM) suggested the formation of particulate polymer composites up to chitosan:gum resin mass ratio of 1:3.
25825076	9	63	theme	resin	1253:1257	arg1	concentration					1259:1271	resin concentration	1253:1271	resin concentration	1253:1271	The drug release mechanism deviated from non-Fickian to case-II type with increasing resin concentration in the composites.
25825076	4	64	theme	particulate	749:759	arg1	composites					769:778	particulate polymer composites	749:778	particulate polymer composites up to chitosan:gum resin mass ratio of 1:3	749:821	Field emission scanning electron microscopy (FE-SEM) suggested the formation of particulate polymer composites up to chitosan:gum resin mass ratio of 1:3.
25825076	1	65	theme	crosslinked	208:218	arg1	composites					237:246	glutaraldehyde (GA) crosslinked chitosan polymer composites	188:246	glutaraldehyde (GA) crosslinked chitosan polymer composites	188:246	This study was undertaken to evaluate the effect of Boswellia gum resin on the properties of glutaraldehyde (GA) crosslinked chitosan polymer composites and their potential as oral delivery vehicles for a non-steroidal anti-inflammatory drug, aceclofenac.
25825076	4	66	theme	gum	795:797	arg1	ratio					810:814	gum resin mass ratio	795:814	particulate polymer composites up to chitosan:gum resin mass ratio of 1:3	749:821	Field emission scanning electron microscopy (FE-SEM) suggested the formation of particulate polymer composites up to chitosan:gum resin mass ratio of 1:3.
25825076	3	67	theme	drug-polymer	634:645	arg1	interaction					656:666	drug-polymer chemical interaction	634:666	drug-polymer chemical interaction	634:666	Fourier transform infrared (FTIR) spectroscopic analysis confirmed the formation of chitosan-gum resin composites and did not show any evidence of drug-polymer chemical interaction.
25825076	2	68	theme	resinous	372:379	arg1	material					381:388	resinous material	372:388	resinous material	372:388	The incorporation of resinous material caused a significant improvement in drug entrapment efficiency (∼40%) of the polymer composites.
25825076	1	69	theme	chitosan	220:227	arg1	composites					237:246	glutaraldehyde (GA) crosslinked chitosan polymer composites	188:246	glutaraldehyde (GA) crosslinked chitosan polymer composites	188:246	This study was undertaken to evaluate the effect of Boswellia gum resin on the properties of glutaraldehyde (GA) crosslinked chitosan polymer composites and their potential as oral delivery vehicles for a non-steroidal anti-inflammatory drug, aceclofenac.
25825076	9	70	theme	drug	1172:1175	arg1	mechanism					1185:1193	The drug release mechanism	1168:1193	The drug release mechanism	1168:1193	The drug release mechanism deviated from non-Fickian to case-II type with increasing resin concentration in the composites.
25825076	4	71	theme	1:3	819:821	arg1	ratio					810:814	gum resin mass ratio	795:814	particulate polymer composites up to chitosan:gum resin mass ratio of 1:3	749:821	Field emission scanning electron microscopy (FE-SEM) suggested the formation of particulate polymer composites up to chitosan:gum resin mass ratio of 1:3.
25825076	2	72	theme	material	381:388	arg1	incorporation					355:367	The incorporation	351:367	The incorporation of resinous material	351:388	The incorporation of resinous material caused a significant improvement in drug entrapment efficiency (∼40%) of the polymer composites.
25825076	4	73	theme	emission	675:682	arg1	FE-SEM					714:719	FE-SEM	714:719	FE-SEM	714:719	Field emission scanning electron microscopy (FE-SEM) suggested the formation of particulate polymer composites up to chitosan:gum resin mass ratio of 1:3.
25825076	4	73	theme	emission	675:682	arg1	microscopy					702:711	Field emission scanning electron microscopy	669:711	Field emission scanning electron microscopy (FE-SEM)	669:720	Field emission scanning electron microscopy (FE-SEM) suggested the formation of particulate polymer composites up to chitosan:gum resin mass ratio of 1:3.
25825076	1	74	theme	polymer	229:235	arg1	composites					237:246	glutaraldehyde (GA) crosslinked chitosan polymer composites	188:246	glutaraldehyde (GA) crosslinked chitosan polymer composites	188:246	This study was undertaken to evaluate the effect of Boswellia gum resin on the properties of glutaraldehyde (GA) crosslinked chitosan polymer composites and their potential as oral delivery vehicles for a non-steroidal anti-inflammatory drug, aceclofenac.
25825076	6	75	theme	release	896:902	arg1	faster					935:940	faster	935:940	faster	935:940	The drug release rate of polymer composites was faster in phosphate buffer solution (pH 6.8).
25825076	6	75	theme	release	896:902	arg1	rate					904:907	The drug release rate	887:907	The drug release rate of polymer composites	887:929	The drug release rate of polymer composites was faster in phosphate buffer solution (pH 6.8).
25825076	6	76	theme	phosphate	945:953	arg1	pH					972:973	pH 6.8	972:977	pH 6.8	972:977	The drug release rate of polymer composites was faster in phosphate buffer solution (pH 6.8).
25825076	6	76	theme	phosphate	945:953	arg1	solution					962:969	phosphate buffer solution	945:969	phosphate buffer solution (pH 6.8)	945:978	The drug release rate of polymer composites was faster in phosphate buffer solution (pH 6.8).
25825076	1	77	theme	Boswellia	147:155	arg1	resin					161:165	Boswellia gum resin	147:165	Boswellia gum resin	147:165	This study was undertaken to evaluate the effect of Boswellia gum resin on the properties of glutaraldehyde (GA) crosslinked chitosan polymer composites and their potential as oral delivery vehicles for a non-steroidal anti-inflammatory drug, aceclofenac.
25825076	6	78	theme	polymer	912:918	arg1	composites					920:929	polymer composites	912:929	polymer composites	912:929	The drug release rate of polymer composites was faster in phosphate buffer solution (pH 6.8).
25825076	1	79	theme	composites	237:246	arg1	properties					174:183	the properties	170:183	the properties of glutaraldehyde (GA) crosslinked chitosan polymer composites	170:246	This study was undertaken to evaluate the effect of Boswellia gum resin on the properties of glutaraldehyde (GA) crosslinked chitosan polymer composites and their potential as oral delivery vehicles for a non-steroidal anti-inflammatory drug, aceclofenac.
25825076	1	79	theme	composites	237:246	arg1	potential					258:266	their potential	252:266	their potential as oral delivery vehicles for a non-steroidal anti-inflammatory drug, aceclofenac	252:348	This study was undertaken to evaluate the effect of Boswellia gum resin on the properties of glutaraldehyde (GA) crosslinked chitosan polymer composites and their potential as oral delivery vehicles for a non-steroidal anti-inflammatory drug, aceclofenac.
28625990	12	0	theme	natural	2642:2648	arg1	lines					2687:2691	natural and genetically modified switchgrass lines	2642:2691	natural and genetically modified switchgrass lines	2642:2691	Here, the extremely thermophilic bacterium Caldicellulosiruptor bescii was used to solubilize natural and genetically modified switchgrass lines, with and without the aid of hydrothermal treatment.
28625990	6	1	theme	microbial	1192:1200	arg1	steps					1202:1206	microbial steps	1192:1206	microbial steps	1192:1206	Hydrothermal treatment (180°C) prior to microbial steps improved solubilization 3.7-fold for the most recalcitrant line (MYB WT) and increased carbohydrate recovery to nearly 50% for the least recalcitrant lines [COMT3(+) and CR].
28625990	11	2	theme	particular	2496:2505	arg1	focus					2507:2511	particular focus	2496:2511	particular focus on lignin content and composition	2496:2545	Recently, transgenic versions of plants and trees have been developed with the intention of lowering the barrier to lignocellulose conversion, with particular focus on lignin content and composition.
28625990	6	3	theme	prior	1183:1187	arg1	180°C					1176:1180	180°C	1176:1180	180°C	1176:1180	Hydrothermal treatment (180°C) prior to microbial steps improved solubilization 3.7-fold for the most recalcitrant line (MYB WT) and increased carbohydrate recovery to nearly 50% for the least recalcitrant lines [COMT3(+) and CR].
28625990	6	3	theme	prior	1183:1187	arg1	treatment					1165:1173	Hydrothermal treatment	1152:1173	Hydrothermal treatment (180°C) prior to microbial steps	1152:1206	Hydrothermal treatment (180°C) prior to microbial steps improved solubilization 3.7-fold for the most recalcitrant line (MYB WT) and increased carbohydrate recovery to nearly 50% for the least recalcitrant lines [COMT3(+) and CR].
28625990	3	4	from	70°C	564:567	arg1	bioavailability					515:529	the bioavailability	511:529	the bioavailability of carbohydrates to C. bescii at 70°C	511:567	Here, the bioavailability of carbohydrates to C. bescii at 70°C was examined for reduced lignin transgenic switchgrass lines COMT3(+) and MYB Trans, their corresponding parental lines (cultivar Alamo) COMT3(-) and MYB wild type (WT), and the natural variant cultivar Cave-in-Rock (CR).
28625990	3	5	theme	lignin	594:599	arg1	lines					624:628	lignin transgenic switchgrass lines	594:628	reduced lignin transgenic switchgrass lines COMT3	586:634	Here, the bioavailability of carbohydrates to C. bescii at 70°C was examined for reduced lignin transgenic switchgrass lines COMT3(+) and MYB Trans, their corresponding parental lines (cultivar Alamo) COMT3(-) and MYB wild type (WT), and the natural variant cultivar Cave-in-Rock (CR).
28625990	3	6	theme	MYB	719:721	arg1	type					728:731	MYB wild type	719:731	MYB wild type	719:731	Here, the bioavailability of carbohydrates to C. bescii at 70°C was examined for reduced lignin transgenic switchgrass lines COMT3(+) and MYB Trans, their corresponding parental lines (cultivar Alamo) COMT3(-) and MYB wild type (WT), and the natural variant cultivar Cave-in-Rock (CR).
28625990	8	7	theme	inert	1754:1758	arg1	materials					1760:1768	inert materials	1754:1768	inert materials (lignin and ash)	1754:1785	Incomplete carbohydrate solubilization suggests that cellulose in the highly lignified residue was inaccessible; indeed, residue from the T→M→T→M treatment was primarily glucan and inert materials (lignin and ash).
28625990	8	7	theme	inert	1754:1758	arg1	ash					1782:1784	ash	1782:1784	ash	1782:1784	Incomplete carbohydrate solubilization suggests that cellulose in the highly lignified residue was inaccessible; indeed, residue from the T→M→T→M treatment was primarily glucan and inert materials (lignin and ash).
28625990	8	7	theme	inert	1754:1758	arg1	lignin					1771:1776	lignin	1771:1776	lignin	1771:1776	Incomplete carbohydrate solubilization suggests that cellulose in the highly lignified residue was inaccessible; indeed, residue from the T→M→T→M treatment was primarily glucan and inert materials (lignin and ash).
28625990	13	8	theme	lignocellulose	2750:2763	arg1	conversion					2765:2774	lignocellulose conversion	2750:2774	lignocellulose conversion	2750:2774	For lignocellulose conversion, it is clear that the microorganism, plant biomass substrate, and processing steps must all be considered simultaneously to achieve optimal results.
28625990	8	9	theme	Incomplete	1573:1582	arg1	solubilization					1597:1610	Incomplete carbohydrate solubilization	1573:1610	Incomplete carbohydrate solubilization	1573:1610	Incomplete carbohydrate solubilization suggests that cellulose in the highly lignified residue was inaccessible; indeed, residue from the T→M→T→M treatment was primarily glucan and inert materials (lignin and ash).
28625990	9	10	dep	solubilization	2066:2079	arg1	access					2116:2121	the organism's access	2101:2121	the organism's access to the carbohydrate content of lignocellulose	2101:2167	While C. bescii could significantly solubilize the transgenic switchgrass lines and natural variant tested here, additional or alternative strategies (physical, chemical, enzymatic, and/or genetic) are needed to eliminate recalcitrance.IMPORTANCE Key to a microbial process for solubilization of plant biomass is the organism's access to the carbohydrate content of lignocellulose.
28625990	12	11	theme	modified	2666:2673	arg1	lines					2687:2691	natural and genetically modified switchgrass lines	2642:2691	natural and genetically modified switchgrass lines	2642:2691	Here, the extremely thermophilic bacterium Caldicellulosiruptor bescii was used to solubilize natural and genetically modified switchgrass lines, with and without the aid of hydrothermal treatment.
28625990	12	12	dep	the	2711:2713	arg1	aid					2715:2717	aid	2715:2717	aid	2715:2717	Here, the extremely thermophilic bacterium Caldicellulosiruptor bescii was used to solubilize natural and genetically modified switchgrass lines, with and without the aid of hydrothermal treatment.
28625990	10	13	theme	physical	2230:2237	arg1	pretreatment					2265:2276	physical, chemical, and biological pretreatment such that microbial steps contribute to the greatest extent possible	2230:2345	physical, chemical, and biological pretreatment such that microbial steps contribute to the greatest extent possible	2230:2345	Economically viable routes will characteristically minimize physical, chemical, and biological pretreatment such that microbial steps contribute to the greatest extent possible.
28625990	12	14	used	used	2623:2626	arg2	bescii					2612:2617	the extremely thermophilic bacterium Caldicellulosiruptor bescii	2554:2617	the extremely thermophilic bacterium Caldicellulosiruptor bescii	2554:2617	Here, the extremely thermophilic bacterium Caldicellulosiruptor bescii was used to solubilize natural and genetically modified switchgrass lines, with and without the aid of hydrothermal treatment.
28625990	9	15	theme	microbial	2044:2052	arg1	process					2054:2060	a microbial process	2042:2060	a microbial process for solubilization of plant biomass is the organism's access to the carbohydrate content of lignocellulose	2042:2167	While C. bescii could significantly solubilize the transgenic switchgrass lines and natural variant tested here, additional or alternative strategies (physical, chemical, enzymatic, and/or genetic) are needed to eliminate recalcitrance.IMPORTANCE Key to a microbial process for solubilization of plant biomass is the organism's access to the carbohydrate content of lignocellulose.
28625990	6	16	theme	recalcitrant	1254:1265	arg1	WT					1277:1278	MYB WT	1273:1278	MYB WT	1273:1278	Hydrothermal treatment (180°C) prior to microbial steps improved solubilization 3.7-fold for the most recalcitrant line (MYB WT) and increased carbohydrate recovery to nearly 50% for the least recalcitrant lines [COMT3(+) and CR].
28625990	6	16	theme	recalcitrant	1254:1265	arg1	line					1267:1270	the most recalcitrant line	1245:1270	the most recalcitrant line (MYB WT)	1245:1279	Hydrothermal treatment (180°C) prior to microbial steps improved solubilization 3.7-fold for the most recalcitrant line (MYB WT) and increased carbohydrate recovery to nearly 50% for the least recalcitrant lines [COMT3(+) and CR].
28625990	3	17	theme	switchgrass	612:622	arg1	lines					624:628	lignin transgenic switchgrass lines	594:628	reduced lignin transgenic switchgrass lines COMT3	586:634	Here, the bioavailability of carbohydrates to C. bescii at 70°C was examined for reduced lignin transgenic switchgrass lines COMT3(+) and MYB Trans, their corresponding parental lines (cultivar Alamo) COMT3(-) and MYB wild type (WT), and the natural variant cultivar Cave-in-Rock (CR).
28625990	7	18	theme	MYB	1532:1534	arg1	WT					1536:1537	MYB WT	1532:1537	MYB WT	1532:1537	Alternating microbial and hydrothermal steps (T→M→T→M) further increased bioavailability, achieving carbohydrate solubilization ranging from 50% for MYB WT to above 70% for COMT3(+) and CR.
28625990	6	19	theme	Hydrothermal	1152:1163	arg1	180°C					1176:1180	180°C	1176:1180	180°C	1176:1180	Hydrothermal treatment (180°C) prior to microbial steps improved solubilization 3.7-fold for the most recalcitrant line (MYB WT) and increased carbohydrate recovery to nearly 50% for the least recalcitrant lines [COMT3(+) and CR].
28625990	6	19	theme	Hydrothermal	1152:1163	arg1	treatment					1165:1173	Hydrothermal treatment	1152:1173	Hydrothermal treatment (180°C) prior to microbial steps	1152:1206	Hydrothermal treatment (180°C) prior to microbial steps improved solubilization 3.7-fold for the most recalcitrant line (MYB WT) and increased carbohydrate recovery to nearly 50% for the least recalcitrant lines [COMT3(+) and CR].
28625990	3	20	theme	variant	755:761	arg1	CR					786:787	CR	786:787	CR	786:787	Here, the bioavailability of carbohydrates to C. bescii at 70°C was examined for reduced lignin transgenic switchgrass lines COMT3(+) and MYB Trans, their corresponding parental lines (cultivar Alamo) COMT3(-) and MYB wild type (WT), and the natural variant cultivar Cave-in-Rock (CR).
28625990	3	20	theme	variant	755:761	arg1	Cave-in-Rock					772:783	the natural variant cultivar Cave-in-Rock	743:783	the natural variant cultivar Cave-in-Rock (CR)	743:788	Here, the bioavailability of carbohydrates to C. bescii at 70°C was examined for reduced lignin transgenic switchgrass lines COMT3(+) and MYB Trans, their corresponding parental lines (cultivar Alamo) COMT3(-) and MYB wild type (WT), and the natural variant cultivar Cave-in-Rock (CR).
28625990	14	21	theme	switchgrass	2933:2943	arg1	lines					2945:2949	switchgrass lines	2933:2949	switchgrass lines engineered for low lignin or natural variants with desirable properties	2933:3021	Whether switchgrass lines engineered for low lignin or natural variants with desirable properties are used, conversion will depend on microbial access to crystalline cellulose in the plant cell wall.
28625990	3	22	theme	natural	747:753	arg1	CR					786:787	CR	786:787	CR	786:787	Here, the bioavailability of carbohydrates to C. bescii at 70°C was examined for reduced lignin transgenic switchgrass lines COMT3(+) and MYB Trans, their corresponding parental lines (cultivar Alamo) COMT3(-) and MYB wild type (WT), and the natural variant cultivar Cave-in-Rock (CR).
28625990	3	22	theme	natural	747:753	arg1	Cave-in-Rock					772:783	the natural variant cultivar Cave-in-Rock	743:783	the natural variant cultivar Cave-in-Rock (CR)	743:788	Here, the bioavailability of carbohydrates to C. bescii at 70°C was examined for reduced lignin transgenic switchgrass lines COMT3(+) and MYB Trans, their corresponding parental lines (cultivar Alamo) COMT3(-) and MYB wild type (WT), and the natural variant cultivar Cave-in-Rock (CR).
28625990	12	23	theme	bacterium	2581:2589	arg1	bescii					2612:2617	the extremely thermophilic bacterium Caldicellulosiruptor bescii	2554:2617	the extremely thermophilic bacterium Caldicellulosiruptor bescii	2554:2617	Here, the extremely thermophilic bacterium Caldicellulosiruptor bescii was used to solubilize natural and genetically modified switchgrass lines, with and without the aid of hydrothermal treatment.
28625990	14	24	from	cellulose	3091:3099	arg1	wall					3119:3122	the plant cell wall	3104:3122	the plant cell wall	3104:3122	Whether switchgrass lines engineered for low lignin or natural variants with desirable properties are used, conversion will depend on microbial access to crystalline cellulose in the plant cell wall.
28625990	14	25	theme	cell	3114:3117	arg1	wall					3119:3122	the plant cell wall	3104:3122	the plant cell wall	3104:3122	Whether switchgrass lines engineered for low lignin or natural variants with desirable properties are used, conversion will depend on microbial access to crystalline cellulose in the plant cell wall.
28625990	10	26	theme	chemical	2240:2247	arg1	pretreatment					2265:2276	physical, chemical, and biological pretreatment such that microbial steps contribute to the greatest extent possible	2230:2345	physical, chemical, and biological pretreatment such that microbial steps contribute to the greatest extent possible	2230:2345	Economically viable routes will characteristically minimize physical, chemical, and biological pretreatment such that microbial steps contribute to the greatest extent possible.
28625990	9	27	theme	transgenic	1839:1848	arg1	lines					1862:1866	transgenic switchgrass lines	1839:1866	transgenic switchgrass lines	1839:1866	While C. bescii could significantly solubilize the transgenic switchgrass lines and natural variant tested here, additional or alternative strategies (physical, chemical, enzymatic, and/or genetic) are needed to eliminate recalcitrance.IMPORTANCE Key to a microbial process for solubilization of plant biomass is the organism's access to the carbohydrate content of lignocellulose.
28625990	9	28	theme	biomass	2090:2096	arg1	solubilization					2066:2079	solubilization	2066:2079	solubilization of plant biomass is the organism's access to the carbohydrate content of lignocellulose	2066:2167	While C. bescii could significantly solubilize the transgenic switchgrass lines and natural variant tested here, additional or alternative strategies (physical, chemical, enzymatic, and/or genetic) are needed to eliminate recalcitrance.IMPORTANCE Key to a microbial process for solubilization of plant biomass is the organism's access to the carbohydrate content of lignocellulose.
28625990	3	29	theme	reduced	586:592	arg1	COMT3					630:634	reduced lignin transgenic switchgrass lines COMT3	586:634	reduced lignin transgenic switchgrass lines COMT3	586:634	Here, the bioavailability of carbohydrates to C. bescii at 70°C was examined for reduced lignin transgenic switchgrass lines COMT3(+) and MYB Trans, their corresponding parental lines (cultivar Alamo) COMT3(-) and MYB wild type (WT), and the natural variant cultivar Cave-in-Rock (CR).
28625990	14	30	with	lignin	2970:2975	arg1	properties					3012:3021	desirable properties	3002:3021	desirable properties	3002:3021	Whether switchgrass lines engineered for low lignin or natural variants with desirable properties are used, conversion will depend on microbial access to crystalline cellulose in the plant cell wall.
28625990	11	31	theme	transgenic	2358:2367	arg1	versions					2369:2376	transgenic versions	2358:2376	transgenic versions of plants and trees	2358:2396	Recently, transgenic versions of plants and trees have been developed with the intention of lowering the barrier to lignocellulose conversion, with particular focus on lignin content and composition.
28625990	8	32	from	treatment	1719:1727	arg1	residue					1694:1700	residue	1694:1700	residue from the T→M→T→M treatment	1694:1727	Incomplete carbohydrate solubilization suggests that cellulose in the highly lignified residue was inaccessible; indeed, residue from the T→M→T→M treatment was primarily glucan and inert materials (lignin and ash).
28625990	6	33	theme	carbohydrate	1295:1306	arg1	recovery					1308:1315	carbohydrate recovery	1295:1315	carbohydrate recovery	1295:1315	Hydrothermal treatment (180°C) prior to microbial steps improved solubilization 3.7-fold for the most recalcitrant line (MYB WT) and increased carbohydrate recovery to nearly 50% for the least recalcitrant lines [COMT3(+) and CR].
28625990	4	34	theme	natural	965:971	arg1	CR					982:983	CR	982:983	CR (36%)	982:989	Transgenic modification improved carbohydrate solubilization by C. bescii to 15% (2.3-fold) for MYB and to 36% (1.5-fold) for COMT, comparable to the levels achieved for the natural variant, CR (36%).
28625990	4	34	theme	natural	965:971	arg1	variant					973:979	the natural variant	961:979	the natural variant	961:979	Transgenic modification improved carbohydrate solubilization by C. bescii to 15% (2.3-fold) for MYB and to 36% (1.5-fold) for COMT, comparable to the levels achieved for the natural variant, CR (36%).
28625990	4	35	theme	comparable	923:932	arg1	COMT					917:920	COMT	917:920	COMT	917:920	Transgenic modification improved carbohydrate solubilization by C. bescii to 15% (2.3-fold) for MYB and to 36% (1.5-fold) for COMT, comparable to the levels achieved for the natural variant, CR (36%).
28625990	9	36	theme	natural	1872:1878	arg1	variant					1880:1886	natural variant	1872:1886	natural variant	1872:1886	While C. bescii could significantly solubilize the transgenic switchgrass lines and natural variant tested here, additional or alternative strategies (physical, chemical, enzymatic, and/or genetic) are needed to eliminate recalcitrance.IMPORTANCE Key to a microbial process for solubilization of plant biomass is the organism's access to the carbohydrate content of lignocellulose.
28625990	14	37	theme	natural	2980:2986	arg1	variants					2988:2995	natural variants	2980:2995	natural variants with desirable properties	2980:3021	Whether switchgrass lines engineered for low lignin or natural variants with desirable properties are used, conversion will depend on microbial access to crystalline cellulose in the plant cell wall.
28625990	5	38	theme	consecutive	1049:1059	arg1	fermentations					1071:1083	two consecutive microbial fermentations	1045:1083	two consecutive microbial fermentations	1045:1083	Carbohydrate solubilization was nearly doubled after two consecutive microbial fermentations compared to one microbial step, but it never exceeded 50% overall.
28625990	1	39	theme	lignocellulose	185:198	arg1	content					174:180	the carbohydrate content	157:180	the carbohydrate content of lignocellulose	157:198	Improving access to the carbohydrate content of lignocellulose is key to reducing recalcitrance for microbial deconstruction and conversion to fuels and chemicals.
28625990	13	40	theme	optimal	2908:2914	arg1	results					2916:2922	optimal results	2908:2922	optimal results	2908:2922	For lignocellulose conversion, it is clear that the microorganism, plant biomass substrate, and processing steps must all be considered simultaneously to achieve optimal results.
28625990	0	41	from	Bioavailability	0:14	arg1	Switchgrasses					66:78	Natural and Transgenic Switchgrasses	43:78	Natural and Transgenic Switchgrasses for the Extreme Thermophile Caldicellulosiruptor bescii	43:134	Bioavailability of Carbohydrate Content in Natural and Transgenic Switchgrasses for the Extreme Thermophile Caldicellulosiruptor bescii.
28625990	0	42	theme	Thermophile	96:106	arg1	bescii					129:134	the Extreme Thermophile Caldicellulosiruptor bescii	84:134	the Extreme Thermophile Caldicellulosiruptor bescii	84:134	Bioavailability of Carbohydrate Content in Natural and Transgenic Switchgrasses for the Extreme Thermophile Caldicellulosiruptor bescii.
28625990	2	43	theme	wall	458:461	arg1	context					432:438	the context	428:438	the context of the plant cell wall	428:461	Caldicellulosiruptor bescii completely solubilizes naked microcrystalline cellulose, yet this transformation is impeded within the context of the plant cell wall by a network of lignin and hemicellulose.
28625990	12	44	theme	hydrothermal	2722:2733	arg1	treatment					2735:2743	hydrothermal treatment	2722:2743	hydrothermal treatment	2722:2743	Here, the extremely thermophilic bacterium Caldicellulosiruptor bescii was used to solubilize natural and genetically modified switchgrass lines, with and without the aid of hydrothermal treatment.
28625990	2	45	theme	plant	447:451	arg1	wall					458:461	the plant cell wall	443:461	the plant cell wall	443:461	Caldicellulosiruptor bescii completely solubilizes naked microcrystalline cellulose, yet this transformation is impeded within the context of the plant cell wall by a network of lignin and hemicellulose.
28625990	5	46	theme	microbial	1101:1109	arg1	step					1111:1114	one microbial step	1097:1114	one microbial step	1097:1114	Carbohydrate solubilization was nearly doubled after two consecutive microbial fermentations compared to one microbial step, but it never exceeded 50% overall.
28625990	10	47	dep	extent	2331:2336	arg1	possible					2338:2345	possible	2338:2345	possible	2338:2345	Economically viable routes will characteristically minimize physical, chemical, and biological pretreatment such that microbial steps contribute to the greatest extent possible.
28625990	10	48	theme	greatest	2322:2329	arg1	extent					2331:2336	the greatest extent	2318:2336	the greatest extent	2318:2336	Economically viable routes will characteristically minimize physical, chemical, and biological pretreatment such that microbial steps contribute to the greatest extent possible.
28625990	14	49	theme	low	2966:2968	arg1	lignin					2970:2975	low lignin	2966:2975	low lignin	2966:2975	Whether switchgrass lines engineered for low lignin or natural variants with desirable properties are used, conversion will depend on microbial access to crystalline cellulose in the plant cell wall.
28625990	0	50	theme	Carbohydrate	19:30	arg1	Content					32:38	Carbohydrate Content	19:38	Carbohydrate Content	19:38	Bioavailability of Carbohydrate Content in Natural and Transgenic Switchgrasses for the Extreme Thermophile Caldicellulosiruptor bescii.
28625990	11	51	from	focus	2507:2511	arg1	composition					2535:2545	composition	2535:2545	composition	2535:2545	Recently, transgenic versions of plants and trees have been developed with the intention of lowering the barrier to lignocellulose conversion, with particular focus on lignin content and composition.
28625990	11	51	from	focus	2507:2511	arg1	content					2523:2529	lignin content	2516:2529	lignin content	2516:2529	Recently, transgenic versions of plants and trees have been developed with the intention of lowering the barrier to lignocellulose conversion, with particular focus on lignin content and composition.
28625990	4	52	theme	Transgenic	791:800	arg1	modification					802:813	Transgenic modification	791:813	Transgenic modification	791:813	Transgenic modification improved carbohydrate solubilization by C. bescii to 15% (2.3-fold) for MYB and to 36% (1.5-fold) for COMT, comparable to the levels achieved for the natural variant, CR (36%).
28625990	10	53	dep	contribute	2304:2313	arg1	such					2278:2281	such	2278:2281	such	2278:2281	Economically viable routes will characteristically minimize physical, chemical, and biological pretreatment such that microbial steps contribute to the greatest extent possible.
28625990	7	54	theme	hydrothermal	1409:1420	arg1	steps					1422:1426	Alternating microbial and hydrothermal steps	1383:1426	Alternating microbial and hydrothermal steps (T→M→T→M)	1383:1436	Alternating microbial and hydrothermal steps (T→M→T→M) further increased bioavailability, achieving carbohydrate solubilization ranging from 50% for MYB WT to above 70% for COMT3(+) and CR.
28625990	7	54	theme	hydrothermal	1409:1420	arg1	T→M→T→M					1429:1435	T→M→T→M	1429:1435	T→M→T→M	1429:1435	Alternating microbial and hydrothermal steps (T→M→T→M) further increased bioavailability, achieving carbohydrate solubilization ranging from 50% for MYB WT to above 70% for COMT3(+) and CR.
28625990	3	55	theme	parental	674:681	arg1	lines					683:687	their corresponding parental lines	654:687	their corresponding parental lines (cultivar Alamo) COMT3	654:710	Here, the bioavailability of carbohydrates to C. bescii at 70°C was examined for reduced lignin transgenic switchgrass lines COMT3(+) and MYB Trans, their corresponding parental lines (cultivar Alamo) COMT3(-) and MYB wild type (WT), and the natural variant cultivar Cave-in-Rock (CR).
28625990	3	55	theme	parental	674:681	arg1	Alamo					699:703	cultivar Alamo	690:703	cultivar Alamo	690:703	Here, the bioavailability of carbohydrates to C. bescii at 70°C was examined for reduced lignin transgenic switchgrass lines COMT3(+) and MYB Trans, their corresponding parental lines (cultivar Alamo) COMT3(-) and MYB wild type (WT), and the natural variant cultivar Cave-in-Rock (CR).
28625990	7	56	theme	microbial	1395:1403	arg1	steps					1422:1426	Alternating microbial and hydrothermal steps	1383:1426	Alternating microbial and hydrothermal steps (T→M→T→M)	1383:1436	Alternating microbial and hydrothermal steps (T→M→T→M) further increased bioavailability, achieving carbohydrate solubilization ranging from 50% for MYB WT to above 70% for COMT3(+) and CR.
28625990	7	56	theme	microbial	1395:1403	arg1	T→M→T→M					1429:1435	T→M→T→M	1429:1435	T→M→T→M	1429:1435	Alternating microbial and hydrothermal steps (T→M→T→M) further increased bioavailability, achieving carbohydrate solubilization ranging from 50% for MYB WT to above 70% for COMT3(+) and CR.
28625990	10	57	theme	viable	2183:2188	arg1	routes					2190:2195	Economically viable routes	2170:2195	Economically viable routes	2170:2195	Economically viable routes will characteristically minimize physical, chemical, and biological pretreatment such that microbial steps contribute to the greatest extent possible.
28625990	8	58	dep	inaccessible	1672:1683	arg1	glucan					1743:1748	glucan	1743:1748	glucan	1743:1748	Incomplete carbohydrate solubilization suggests that cellulose in the highly lignified residue was inaccessible; indeed, residue from the T→M→T→M treatment was primarily glucan and inert materials (lignin and ash).
28625990	8	58	dep	inaccessible	1672:1683	arg1	materials					1760:1768	inert materials	1754:1768	inert materials (lignin and ash)	1754:1785	Incomplete carbohydrate solubilization suggests that cellulose in the highly lignified residue was inaccessible; indeed, residue from the T→M→T→M treatment was primarily glucan and inert materials (lignin and ash).
28625990	8	58	dep	inaccessible	1672:1683	arg1	ash					1782:1784	ash	1782:1784	ash	1782:1784	Incomplete carbohydrate solubilization suggests that cellulose in the highly lignified residue was inaccessible; indeed, residue from the T→M→T→M treatment was primarily glucan and inert materials (lignin and ash).
28625990	8	58	dep	inaccessible	1672:1683	arg1	lignin					1771:1776	lignin	1771:1776	lignin	1771:1776	Incomplete carbohydrate solubilization suggests that cellulose in the highly lignified residue was inaccessible; indeed, residue from the T→M→T→M treatment was primarily glucan and inert materials (lignin and ash).
28625990	13	59	theme	biomass	2819:2825	arg1	substrate					2827:2835	plant biomass substrate	2813:2835	plant biomass substrate	2813:2835	For lignocellulose conversion, it is clear that the microorganism, plant biomass substrate, and processing steps must all be considered simultaneously to achieve optimal results.
28625990	8	60	dep	materials	1760:1768	arg1	materials					1760:1768	inert materials	1754:1768	inert materials (lignin and ash)	1754:1785	Incomplete carbohydrate solubilization suggests that cellulose in the highly lignified residue was inaccessible; indeed, residue from the T→M→T→M treatment was primarily glucan and inert materials (lignin and ash).
28625990	8	60	dep	materials	1760:1768	arg1	ash					1782:1784	ash	1782:1784	ash	1782:1784	Incomplete carbohydrate solubilization suggests that cellulose in the highly lignified residue was inaccessible; indeed, residue from the T→M→T→M treatment was primarily glucan and inert materials (lignin and ash).
28625990	8	60	dep	materials	1760:1768	arg1	lignin					1771:1776	lignin	1771:1776	lignin	1771:1776	Incomplete carbohydrate solubilization suggests that cellulose in the highly lignified residue was inaccessible; indeed, residue from the T→M→T→M treatment was primarily glucan and inert materials (lignin and ash).
28625990	3	61	theme	wild	723:726	arg1	type					728:731	MYB wild type	719:731	MYB wild type	719:731	Here, the bioavailability of carbohydrates to C. bescii at 70°C was examined for reduced lignin transgenic switchgrass lines COMT3(+) and MYB Trans, their corresponding parental lines (cultivar Alamo) COMT3(-) and MYB wild type (WT), and the natural variant cultivar Cave-in-Rock (CR).
28625990	9	62	dep	strategies	1927:1936	arg1	enzymatic					1959:1967	enzymatic	1959:1967	enzymatic	1959:1967	While C. bescii could significantly solubilize the transgenic switchgrass lines and natural variant tested here, additional or alternative strategies (physical, chemical, enzymatic, and/or genetic) are needed to eliminate recalcitrance.IMPORTANCE Key to a microbial process for solubilization of plant biomass is the organism's access to the carbohydrate content of lignocellulose.
28625990	9	62	dep	strategies	1927:1936	arg1	genetic					1977:1983	genetic	1977:1983	genetic	1977:1983	While C. bescii could significantly solubilize the transgenic switchgrass lines and natural variant tested here, additional or alternative strategies (physical, chemical, enzymatic, and/or genetic) are needed to eliminate recalcitrance.IMPORTANCE Key to a microbial process for solubilization of plant biomass is the organism's access to the carbohydrate content of lignocellulose.
28625990	9	62	dep	strategies	1927:1936	arg1	chemical					1949:1956	chemical	1949:1956	chemical	1949:1956	While C. bescii could significantly solubilize the transgenic switchgrass lines and natural variant tested here, additional or alternative strategies (physical, chemical, enzymatic, and/or genetic) are needed to eliminate recalcitrance.IMPORTANCE Key to a microbial process for solubilization of plant biomass is the organism's access to the carbohydrate content of lignocellulose.
28625990	9	62	dep	strategies	1927:1936	arg1	physical					1939:1946	physical	1939:1946	physical	1939:1946	While C. bescii could significantly solubilize the transgenic switchgrass lines and natural variant tested here, additional or alternative strategies (physical, chemical, enzymatic, and/or genetic) are needed to eliminate recalcitrance.IMPORTANCE Key to a microbial process for solubilization of plant biomass is the organism's access to the carbohydrate content of lignocellulose.
28625990	12	63	theme	switchgrass	2675:2685	arg1	lines					2687:2691	natural and genetically modified switchgrass lines	2642:2691	natural and genetically modified switchgrass lines	2642:2691	Here, the extremely thermophilic bacterium Caldicellulosiruptor bescii was used to solubilize natural and genetically modified switchgrass lines, with and without the aid of hydrothermal treatment.
28625990	11	64	theme	lignin	2516:2521	arg1	content					2523:2529	lignin content	2516:2529	lignin content	2516:2529	Recently, transgenic versions of plants and trees have been developed with the intention of lowering the barrier to lignocellulose conversion, with particular focus on lignin content and composition.
28625990	2	65	theme	naked	352:356	arg1	cellulose					375:383	naked microcrystalline cellulose	352:383	naked microcrystalline cellulose	352:383	Caldicellulosiruptor bescii completely solubilizes naked microcrystalline cellulose, yet this transformation is impeded within the context of the plant cell wall by a network of lignin and hemicellulose.
28625990	14	66	theme	microbial	3059:3067	arg1	access					3069:3074	microbial access	3059:3074	microbial access to crystalline cellulose in the plant cell wall	3059:3122	Whether switchgrass lines engineered for low lignin or natural variants with desirable properties are used, conversion will depend on microbial access to crystalline cellulose in the plant cell wall.
28625990	4	67	theme	carbohydrate	824:835	arg1	solubilization					837:850	carbohydrate solubilization	824:850	carbohydrate solubilization	824:850	Transgenic modification improved carbohydrate solubilization by C. bescii to 15% (2.3-fold) for MYB and to 36% (1.5-fold) for COMT, comparable to the levels achieved for the natural variant, CR (36%).
28625990	9	68	theme	Key	2035:2037	arg1	recalcitrance.IMPORTANCE					2010:2033	recalcitrance.IMPORTANCE	2010:2033	recalcitrance.IMPORTANCE Key to a microbial process for solubilization of plant biomass is the organism's access to the carbohydrate content of lignocellulose	2010:2167	While C. bescii could significantly solubilize the transgenic switchgrass lines and natural variant tested here, additional or alternative strategies (physical, chemical, enzymatic, and/or genetic) are needed to eliminate recalcitrance.IMPORTANCE Key to a microbial process for solubilization of plant biomass is the organism's access to the carbohydrate content of lignocellulose.
28625990	3	69	theme	lines	624:628	arg1	COMT3					630:634	reduced lignin transgenic switchgrass lines COMT3	586:634	reduced lignin transgenic switchgrass lines COMT3	586:634	Here, the bioavailability of carbohydrates to C. bescii at 70°C was examined for reduced lignin transgenic switchgrass lines COMT3(+) and MYB Trans, their corresponding parental lines (cultivar Alamo) COMT3(-) and MYB wild type (WT), and the natural variant cultivar Cave-in-Rock (CR).
28625990	13	70	theme	processing	2842:2851	arg1	steps					2853:2857	processing steps	2842:2857	processing steps	2842:2857	For lignocellulose conversion, it is clear that the microorganism, plant biomass substrate, and processing steps must all be considered simultaneously to achieve optimal results.
28625990	8	71	from	cellulose	1626:1634	arg1	residue					1660:1666	the highly lignified residue	1639:1666	the highly lignified residue	1639:1666	Incomplete carbohydrate solubilization suggests that cellulose in the highly lignified residue was inaccessible; indeed, residue from the T→M→T→M treatment was primarily glucan and inert materials (lignin and ash).
28625990	3	72	theme	transgenic	601:610	arg1	lines					624:628	lignin transgenic switchgrass lines	594:628	reduced lignin transgenic switchgrass lines COMT3	586:634	Here, the bioavailability of carbohydrates to C. bescii at 70°C was examined for reduced lignin transgenic switchgrass lines COMT3(+) and MYB Trans, their corresponding parental lines (cultivar Alamo) COMT3(-) and MYB wild type (WT), and the natural variant cultivar Cave-in-Rock (CR).
28625990	8	73	theme	carbohydrate	1584:1595	arg1	solubilization					1597:1610	Incomplete carbohydrate solubilization	1573:1610	Incomplete carbohydrate solubilization	1573:1610	Incomplete carbohydrate solubilization suggests that cellulose in the highly lignified residue was inaccessible; indeed, residue from the T→M→T→M treatment was primarily glucan and inert materials (lignin and ash).
28625990	9	74	dep	lines	1862:1866	arg1	the					1835:1837	the	1835:1837	the	1835:1837	While C. bescii could significantly solubilize the transgenic switchgrass lines and natural variant tested here, additional or alternative strategies (physical, chemical, enzymatic, and/or genetic) are needed to eliminate recalcitrance.IMPORTANCE Key to a microbial process for solubilization of plant biomass is the organism's access to the carbohydrate content of lignocellulose.
28625990	1	75	theme	microbial	237:245	arg1	deconstruction					247:260	microbial deconstruction	237:260	microbial deconstruction	237:260	Improving access to the carbohydrate content of lignocellulose is key to reducing recalcitrance for microbial deconstruction and conversion to fuels and chemicals.
28625990	14	76	with	variants	2988:2995	arg1	properties					3012:3021	desirable properties	3002:3021	desirable properties	3002:3021	Whether switchgrass lines engineered for low lignin or natural variants with desirable properties are used, conversion will depend on microbial access to crystalline cellulose in the plant cell wall.
28625990	3	77	theme	cultivar	763:770	arg1	CR					786:787	CR	786:787	CR	786:787	Here, the bioavailability of carbohydrates to C. bescii at 70°C was examined for reduced lignin transgenic switchgrass lines COMT3(+) and MYB Trans, their corresponding parental lines (cultivar Alamo) COMT3(-) and MYB wild type (WT), and the natural variant cultivar Cave-in-Rock (CR).
28625990	3	77	theme	cultivar	763:770	arg1	Cave-in-Rock					772:783	the natural variant cultivar Cave-in-Rock	743:783	the natural variant cultivar Cave-in-Rock (CR)	743:788	Here, the bioavailability of carbohydrates to C. bescii at 70°C was examined for reduced lignin transgenic switchgrass lines COMT3(+) and MYB Trans, their corresponding parental lines (cultivar Alamo) COMT3(-) and MYB wild type (WT), and the natural variant cultivar Cave-in-Rock (CR).
28625990	5	78	theme	Carbohydrate	992:1003	arg1	solubilization					1005:1018	Carbohydrate solubilization	992:1018	Carbohydrate solubilization	992:1018	Carbohydrate solubilization was nearly doubled after two consecutive microbial fermentations compared to one microbial step, but it never exceeded 50% overall.
28625990	12	79	theme	thermophilic	2568:2579	arg1	bescii					2612:2617	the extremely thermophilic bacterium Caldicellulosiruptor bescii	2554:2617	the extremely thermophilic bacterium Caldicellulosiruptor bescii	2554:2617	Here, the extremely thermophilic bacterium Caldicellulosiruptor bescii was used to solubilize natural and genetically modified switchgrass lines, with and without the aid of hydrothermal treatment.
28625990	9	80	theme	plant	2084:2088	arg1	biomass					2090:2096	plant biomass	2084:2096	plant biomass	2084:2096	While C. bescii could significantly solubilize the transgenic switchgrass lines and natural variant tested here, additional or alternative strategies (physical, chemical, enzymatic, and/or genetic) are needed to eliminate recalcitrance.IMPORTANCE Key to a microbial process for solubilization of plant biomass is the organism's access to the carbohydrate content of lignocellulose.
28625990	2	81	theme	Caldicellulosiruptor	301:320	arg1	bescii					322:327	Caldicellulosiruptor bescii	301:327	Caldicellulosiruptor bescii	301:327	Caldicellulosiruptor bescii completely solubilizes naked microcrystalline cellulose, yet this transformation is impeded within the context of the plant cell wall by a network of lignin and hemicellulose.
28625990	7	82	theme	carbohydrate	1483:1494	arg1	solubilization					1496:1509	carbohydrate solubilization	1483:1509	carbohydrate solubilization ranging from 50% for MYB WT to above 70% for COMT3(+) and CR	1483:1570	Alternating microbial and hydrothermal steps (T→M→T→M) further increased bioavailability, achieving carbohydrate solubilization ranging from 50% for MYB WT to above 70% for COMT3(+) and CR.
28625990	12	83	theme	Caldicellulosiruptor	2591:2610	arg1	bescii					2612:2617	the extremely thermophilic bacterium Caldicellulosiruptor bescii	2554:2617	the extremely thermophilic bacterium Caldicellulosiruptor bescii	2554:2617	Here, the extremely thermophilic bacterium Caldicellulosiruptor bescii was used to solubilize natural and genetically modified switchgrass lines, with and without the aid of hydrothermal treatment.
28625990	9	84	theme	switchgrass	1850:1860	arg1	lines					1862:1866	transgenic switchgrass lines	1839:1866	transgenic switchgrass lines	1839:1866	While C. bescii could significantly solubilize the transgenic switchgrass lines and natural variant tested here, additional or alternative strategies (physical, chemical, enzymatic, and/or genetic) are needed to eliminate recalcitrance.IMPORTANCE Key to a microbial process for solubilization of plant biomass is the organism's access to the carbohydrate content of lignocellulose.
28625990	14	85	theme	desirable	3002:3010	arg1	properties					3012:3021	desirable properties	3002:3021	desirable properties	3002:3021	Whether switchgrass lines engineered for low lignin or natural variants with desirable properties are used, conversion will depend on microbial access to crystalline cellulose in the plant cell wall.
28625990	3	86	theme	MYB	643:645	arg1	Trans					647:651	MYB Trans	643:651	MYB Trans	643:651	Here, the bioavailability of carbohydrates to C. bescii at 70°C was examined for reduced lignin transgenic switchgrass lines COMT3(+) and MYB Trans, their corresponding parental lines (cultivar Alamo) COMT3(-) and MYB wild type (WT), and the natural variant cultivar Cave-in-Rock (CR).
28625990	8	87	theme	lignified	1650:1658	arg1	residue					1660:1666	the highly lignified residue	1639:1666	the highly lignified residue	1639:1666	Incomplete carbohydrate solubilization suggests that cellulose in the highly lignified residue was inaccessible; indeed, residue from the T→M→T→M treatment was primarily glucan and inert materials (lignin and ash).
28625990	1	88	theme	carbohydrate	161:172	arg1	content					174:180	the carbohydrate content	157:180	the carbohydrate content of lignocellulose	157:198	Improving access to the carbohydrate content of lignocellulose is key to reducing recalcitrance for microbial deconstruction and conversion to fuels and chemicals.
28625990	11	89	theme	plants	2381:2386	arg1	versions					2369:2376	transgenic versions	2358:2376	transgenic versions of plants and trees	2358:2396	Recently, transgenic versions of plants and trees have been developed with the intention of lowering the barrier to lignocellulose conversion, with particular focus on lignin content and composition.
28625990	0	90	theme	Extreme	88:94	arg1	bescii					129:134	the Extreme Thermophile Caldicellulosiruptor bescii	84:134	the Extreme Thermophile Caldicellulosiruptor bescii	84:134	Bioavailability of Carbohydrate Content in Natural and Transgenic Switchgrasses for the Extreme Thermophile Caldicellulosiruptor bescii.
28625990	10	91	theme	biological	2254:2263	arg1	pretreatment					2265:2276	physical, chemical, and biological pretreatment such that microbial steps contribute to the greatest extent possible	2230:2345	physical, chemical, and biological pretreatment such that microbial steps contribute to the greatest extent possible	2230:2345	Economically viable routes will characteristically minimize physical, chemical, and biological pretreatment such that microbial steps contribute to the greatest extent possible.
28625990	0	92	theme	Caldicellulosiruptor	108:127	arg1	bescii					129:134	the Extreme Thermophile Caldicellulosiruptor bescii	84:134	the Extreme Thermophile Caldicellulosiruptor bescii	84:134	Bioavailability of Carbohydrate Content in Natural and Transgenic Switchgrasses for the Extreme Thermophile Caldicellulosiruptor bescii.
28625990	5	93	theme	microbial	1061:1069	arg1	fermentations					1071:1083	two consecutive microbial fermentations	1045:1083	two consecutive microbial fermentations	1045:1083	Carbohydrate solubilization was nearly doubled after two consecutive microbial fermentations compared to one microbial step, but it never exceeded 50% overall.
28625990	2	94	theme	microcrystalline	358:373	arg1	cellulose					375:383	naked microcrystalline cellulose	352:383	naked microcrystalline cellulose	352:383	Caldicellulosiruptor bescii completely solubilizes naked microcrystalline cellulose, yet this transformation is impeded within the context of the plant cell wall by a network of lignin and hemicellulose.
28625990	6	95	theme	MYB	1273:1275	arg1	WT					1277:1278	MYB WT	1273:1278	MYB WT	1273:1278	Hydrothermal treatment (180°C) prior to microbial steps improved solubilization 3.7-fold for the most recalcitrant line (MYB WT) and increased carbohydrate recovery to nearly 50% for the least recalcitrant lines [COMT3(+) and CR].
28625990	6	95	theme	MYB	1273:1275	arg1	line					1267:1270	the most recalcitrant line	1245:1270	the most recalcitrant line (MYB WT)	1245:1279	Hydrothermal treatment (180°C) prior to microbial steps improved solubilization 3.7-fold for the most recalcitrant line (MYB WT) and increased carbohydrate recovery to nearly 50% for the least recalcitrant lines [COMT3(+) and CR].
28625990	6	96	dep	increased	1285:1293	arg1	CR					1378:1379	CR	1378:1379	CR	1378:1379	Hydrothermal treatment (180°C) prior to microbial steps improved solubilization 3.7-fold for the most recalcitrant line (MYB WT) and increased carbohydrate recovery to nearly 50% for the least recalcitrant lines [COMT3(+) and CR].
28625990	6	96	dep	increased	1285:1293	arg1	[COMT3					1364:1369	[COMT3(+)	1364:1372	[COMT3	1364:1369	Hydrothermal treatment (180°C) prior to microbial steps improved solubilization 3.7-fold for the most recalcitrant line (MYB WT) and increased carbohydrate recovery to nearly 50% for the least recalcitrant lines [COMT3(+) and CR].
28625990	10	97	theme	microbial	2288:2296	arg1	steps					2298:2302	microbial steps	2288:2302	microbial steps	2288:2302	Economically viable routes will characteristically minimize physical, chemical, and biological pretreatment such that microbial steps contribute to the greatest extent possible.
28625990	11	98	theme	trees	2392:2396	arg1	versions					2369:2376	transgenic versions	2358:2376	transgenic versions of plants and trees	2358:2396	Recently, transgenic versions of plants and trees have been developed with the intention of lowering the barrier to lignocellulose conversion, with particular focus on lignin content and composition.
28625990	9	99	theme	additional	1901:1910	arg1	strategies					1927:1936	additional or alternative strategies	1901:1936	additional or alternative strategies (physical, chemical, enzymatic, and/or genetic)	1901:1984	While C. bescii could significantly solubilize the transgenic switchgrass lines and natural variant tested here, additional or alternative strategies (physical, chemical, enzymatic, and/or genetic) are needed to eliminate recalcitrance.IMPORTANCE Key to a microbial process for solubilization of plant biomass is the organism's access to the carbohydrate content of lignocellulose.
28625990	2	100	theme	cell	453:456	arg1	wall					458:461	the plant cell wall	443:461	the plant cell wall	443:461	Caldicellulosiruptor bescii completely solubilizes naked microcrystalline cellulose, yet this transformation is impeded within the context of the plant cell wall by a network of lignin and hemicellulose.
28625990	6	101	theme	recalcitrant	1345:1356	arg1	lines					1358:1362	the least recalcitrant lines	1335:1362	the least recalcitrant lines	1335:1362	Hydrothermal treatment (180°C) prior to microbial steps improved solubilization 3.7-fold for the most recalcitrant line (MYB WT) and increased carbohydrate recovery to nearly 50% for the least recalcitrant lines [COMT3(+) and CR].
28625990	14	102	used	used	3027:3030	arg2	lines					2945:2949	switchgrass lines	2933:2949	switchgrass lines engineered for low lignin or natural variants with desirable properties	2933:3021	Whether switchgrass lines engineered for low lignin or natural variants with desirable properties are used, conversion will depend on microbial access to crystalline cellulose in the plant cell wall.
28625990	2	103	theme	hemicellulose	490:502	arg1	network					468:474	a network	466:474	a network of lignin and hemicellulose	466:502	Caldicellulosiruptor bescii completely solubilizes naked microcrystalline cellulose, yet this transformation is impeded within the context of the plant cell wall by a network of lignin and hemicellulose.
28625990	9	104	theme	alternative	1915:1925	arg1	strategies					1927:1936	additional or alternative strategies	1901:1936	additional or alternative strategies (physical, chemical, enzymatic, and/or genetic)	1901:1984	While C. bescii could significantly solubilize the transgenic switchgrass lines and natural variant tested here, additional or alternative strategies (physical, chemical, enzymatic, and/or genetic) are needed to eliminate recalcitrance.IMPORTANCE Key to a microbial process for solubilization of plant biomass is the organism's access to the carbohydrate content of lignocellulose.
28625990	9	105	theme	lignocellulose	2154:2167	arg1	content					2143:2149	the carbohydrate content	2126:2149	the carbohydrate content of lignocellulose	2126:2167	While C. bescii could significantly solubilize the transgenic switchgrass lines and natural variant tested here, additional or alternative strategies (physical, chemical, enzymatic, and/or genetic) are needed to eliminate recalcitrance.IMPORTANCE Key to a microbial process for solubilization of plant biomass is the organism's access to the carbohydrate content of lignocellulose.
28625990	0	106	theme	Content	32:38	arg1	Bioavailability					0:14	Bioavailability	0:14	Bioavailability of Carbohydrate Content in Natural and Transgenic Switchgrasses for the Extreme Thermophile Caldicellulosiruptor bescii.	0:135	Bioavailability of Carbohydrate Content in Natural and Transgenic Switchgrasses for the Extreme Thermophile Caldicellulosiruptor bescii.
28625990	8	107	theme	T→M→T→M	1711:1717	arg1	treatment					1719:1727	the T→M→T→M treatment	1707:1727	the T→M→T→M treatment	1707:1727	Incomplete carbohydrate solubilization suggests that cellulose in the highly lignified residue was inaccessible; indeed, residue from the T→M→T→M treatment was primarily glucan and inert materials (lignin and ash).
28625990	2	108	theme	lignin	479:484	arg1	network					468:474	a network	466:474	a network of lignin and hemicellulose	466:502	Caldicellulosiruptor bescii completely solubilizes naked microcrystalline cellulose, yet this transformation is impeded within the context of the plant cell wall by a network of lignin and hemicellulose.
28625990	3	109	theme	lines	683:687	arg1	WT					734:735	WT	734:735	WT	734:735	Here, the bioavailability of carbohydrates to C. bescii at 70°C was examined for reduced lignin transgenic switchgrass lines COMT3(+) and MYB Trans, their corresponding parental lines (cultivar Alamo) COMT3(-) and MYB wild type (WT), and the natural variant cultivar Cave-in-Rock (CR).
28625990	3	109	theme	lines	683:687	arg1	COMT3					706:710	their corresponding parental lines (cultivar Alamo) COMT3	654:710	their corresponding parental lines (cultivar Alamo) COMT3	654:710	Here, the bioavailability of carbohydrates to C. bescii at 70°C was examined for reduced lignin transgenic switchgrass lines COMT3(+) and MYB Trans, their corresponding parental lines (cultivar Alamo) COMT3(-) and MYB wild type (WT), and the natural variant cultivar Cave-in-Rock (CR).
28625990	0	110	theme	Natural	43:49	arg1	Switchgrasses					66:78	Natural and Transgenic Switchgrasses	43:78	Natural and Transgenic Switchgrasses for the Extreme Thermophile Caldicellulosiruptor bescii	43:134	Bioavailability of Carbohydrate Content in Natural and Transgenic Switchgrasses for the Extreme Thermophile Caldicellulosiruptor bescii.
28625990	9	111	theme	carbohydrate	2130:2141	arg1	content					2143:2149	the carbohydrate content	2126:2149	the carbohydrate content of lignocellulose	2126:2167	While C. bescii could significantly solubilize the transgenic switchgrass lines and natural variant tested here, additional or alternative strategies (physical, chemical, enzymatic, and/or genetic) are needed to eliminate recalcitrance.IMPORTANCE Key to a microbial process for solubilization of plant biomass is the organism's access to the carbohydrate content of lignocellulose.
28625990	3	112	theme	corresponding	660:672	arg1	lines					683:687	their corresponding parental lines	654:687	their corresponding parental lines (cultivar Alamo) COMT3	654:710	Here, the bioavailability of carbohydrates to C. bescii at 70°C was examined for reduced lignin transgenic switchgrass lines COMT3(+) and MYB Trans, their corresponding parental lines (cultivar Alamo) COMT3(-) and MYB wild type (WT), and the natural variant cultivar Cave-in-Rock (CR).
28625990	3	112	theme	corresponding	660:672	arg1	Alamo					699:703	cultivar Alamo	690:703	cultivar Alamo	690:703	Here, the bioavailability of carbohydrates to C. bescii at 70°C was examined for reduced lignin transgenic switchgrass lines COMT3(+) and MYB Trans, their corresponding parental lines (cultivar Alamo) COMT3(-) and MYB wild type (WT), and the natural variant cultivar Cave-in-Rock (CR).
28625990	13	113	theme	plant	2813:2817	arg1	substrate					2827:2835	plant biomass substrate	2813:2835	plant biomass substrate	2813:2835	For lignocellulose conversion, it is clear that the microorganism, plant biomass substrate, and processing steps must all be considered simultaneously to achieve optimal results.
28625990	0	114	theme	Transgenic	55:64	arg1	Switchgrasses					66:78	Natural and Transgenic Switchgrasses	43:78	Natural and Transgenic Switchgrasses for the Extreme Thermophile Caldicellulosiruptor bescii	43:134	Bioavailability of Carbohydrate Content in Natural and Transgenic Switchgrasses for the Extreme Thermophile Caldicellulosiruptor bescii.
28625990	14	115	theme	plant	3108:3112	arg1	wall					3119:3122	the plant cell wall	3104:3122	the plant cell wall	3104:3122	Whether switchgrass lines engineered for low lignin or natural variants with desirable properties are used, conversion will depend on microbial access to crystalline cellulose in the plant cell wall.
28625990	3	116	theme	cultivar	690:697	arg1	lines					683:687	their corresponding parental lines	654:687	their corresponding parental lines (cultivar Alamo) COMT3	654:710	Here, the bioavailability of carbohydrates to C. bescii at 70°C was examined for reduced lignin transgenic switchgrass lines COMT3(+) and MYB Trans, their corresponding parental lines (cultivar Alamo) COMT3(-) and MYB wild type (WT), and the natural variant cultivar Cave-in-Rock (CR).
28625990	3	116	theme	cultivar	690:697	arg1	Alamo					699:703	cultivar Alamo	690:703	cultivar Alamo	690:703	Here, the bioavailability of carbohydrates to C. bescii at 70°C was examined for reduced lignin transgenic switchgrass lines COMT3(+) and MYB Trans, their corresponding parental lines (cultivar Alamo) COMT3(-) and MYB wild type (WT), and the natural variant cultivar Cave-in-Rock (CR).
28625990	3	117	theme	carbohydrates	534:546	arg1	bioavailability					515:529	the bioavailability	511:529	the bioavailability of carbohydrates to C. bescii at 70°C	511:567	Here, the bioavailability of carbohydrates to C. bescii at 70°C was examined for reduced lignin transgenic switchgrass lines COMT3(+) and MYB Trans, their corresponding parental lines (cultivar Alamo) COMT3(-) and MYB wild type (WT), and the natural variant cultivar Cave-in-Rock (CR).
28625990	5	118	theme	overall	1143:1149	arg1	%					1141:1141	50% overall	1139:1149	50% overall	1139:1149	Carbohydrate solubilization was nearly doubled after two consecutive microbial fermentations compared to one microbial step, but it never exceeded 50% overall.
28625990	7	119	theme	Alternating	1383:1393	arg1	steps					1422:1426	Alternating microbial and hydrothermal steps	1383:1426	Alternating microbial and hydrothermal steps (T→M→T→M)	1383:1436	Alternating microbial and hydrothermal steps (T→M→T→M) further increased bioavailability, achieving carbohydrate solubilization ranging from 50% for MYB WT to above 70% for COMT3(+) and CR.
28625990	7	119	theme	Alternating	1383:1393	arg1	T→M→T→M					1429:1435	T→M→T→M	1429:1435	T→M→T→M	1429:1435	Alternating microbial and hydrothermal steps (T→M→T→M) further increased bioavailability, achieving carbohydrate solubilization ranging from 50% for MYB WT to above 70% for COMT3(+) and CR.
28625990	11	120	theme	lignocellulose	2464:2477	arg1	conversion					2479:2488	lignocellulose conversion	2464:2488	lignocellulose conversion	2464:2488	Recently, transgenic versions of plants and trees have been developed with the intention of lowering the barrier to lignocellulose conversion, with particular focus on lignin content and composition.
28625990	14	121	theme	crystalline	3079:3089	arg1	cellulose					3091:3099	crystalline cellulose	3079:3099	crystalline cellulose in the plant cell wall	3079:3122	Whether switchgrass lines engineered for low lignin or natural variants with desirable properties are used, conversion will depend on microbial access to crystalline cellulose in the plant cell wall.
26686161	4	0	theme	peroxidase	1000:1009	arg1	levels					1069:1074	lower levels	1063:1074	lower levels of malondialdehyde in serums and livers compared to the d-galactose-treated group	1063:1156	Furthermore, it was demonstrated that PHMPs had a significant protective effect against oxidative stress induced by d-galactose in mice, as evident by higher activities of superoxide dismutase, catalase, glutathione peroxidase and level of total antioxidant capacity, as well as lower levels of malondialdehyde in serums and livers compared to the d-galactose-treated group.
26686161	4	0	theme	peroxidase	1000:1009	arg1	activities					942:951	higher activities	935:951	higher activities of superoxide dismutase, catalase, glutathione peroxidase and level of total antioxidant capacity	935:1049	Furthermore, it was demonstrated that PHMPs had a significant protective effect against oxidative stress induced by d-galactose in mice, as evident by higher activities of superoxide dismutase, catalase, glutathione peroxidase and level of total antioxidant capacity, as well as lower levels of malondialdehyde in serums and livers compared to the d-galactose-treated group.
26686161	4	1	from	malondialdehyde	1079:1093	arg1	livers					1109:1114	livers	1109:1114	livers	1109:1114	Furthermore, it was demonstrated that PHMPs had a significant protective effect against oxidative stress induced by d-galactose in mice, as evident by higher activities of superoxide dismutase, catalase, glutathione peroxidase and level of total antioxidant capacity, as well as lower levels of malondialdehyde in serums and livers compared to the d-galactose-treated group.
26686161	4	1	from	malondialdehyde	1079:1093	arg1	serums					1098:1103	serums	1098:1103	serums	1098:1103	Furthermore, it was demonstrated that PHMPs had a significant protective effect against oxidative stress induced by d-galactose in mice, as evident by higher activities of superoxide dismutase, catalase, glutathione peroxidase and level of total antioxidant capacity, as well as lower levels of malondialdehyde in serums and livers compared to the d-galactose-treated group.
26686161	3	2	theme	composition	502:512	arg1	analysis					475:482	The analysis	471:482	The analysis of monosaccharide composition by high performance liquid chromatography (HPLC)	471:561	The analysis of monosaccharide composition by high performance liquid chromatography (HPLC) revealed that PHMPs was composed of mannose, ribose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose and arabinose in molar ratio of 46.07:0.59:2.25:1.29:1.42:18.82:26.17:1.13:2.26, respectively.
26686161	4	3	from	serums	1098:1103	arg1	levels					1069:1074	lower levels	1063:1074	lower levels of malondialdehyde in serums and livers compared to the d-galactose-treated group	1063:1156	Furthermore, it was demonstrated that PHMPs had a significant protective effect against oxidative stress induced by d-galactose in mice, as evident by higher activities of superoxide dismutase, catalase, glutathione peroxidase and level of total antioxidant capacity, as well as lower levels of malondialdehyde in serums and livers compared to the d-galactose-treated group.
26686161	4	3	from	serums	1098:1103	arg1	activities					942:951	higher activities	935:951	higher activities of superoxide dismutase, catalase, glutathione peroxidase and level of total antioxidant capacity	935:1049	Furthermore, it was demonstrated that PHMPs had a significant protective effect against oxidative stress induced by d-galactose in mice, as evident by higher activities of superoxide dismutase, catalase, glutathione peroxidase and level of total antioxidant capacity, as well as lower levels of malondialdehyde in serums and livers compared to the d-galactose-treated group.
26686161	0	4	theme	Paecilomyces	87:98	arg1	hepiali					100:106	Paecilomyces hepiali	87:106	Paecilomyces hepiali	87:106	Extraction, characterization and antioxidant activity of mycelial polysaccharides from Paecilomyces hepiali HN1.
26686161	1	5	theme	mycelial	186:193	arg1	polysaccharides					195:209	mycelial polysaccharides	186:209	mycelial polysaccharides from Paecilomyces hepiali HN1 (PHMPs)	186:247	Optimization of extraction, characterization and antioxidant activity of mycelial polysaccharides from Paecilomyces hepiali HN1 (PHMPs) were investigated.
26686161	4	6	from	activities	942:951	arg1	livers					1109:1114	livers	1109:1114	livers	1109:1114	Furthermore, it was demonstrated that PHMPs had a significant protective effect against oxidative stress induced by d-galactose in mice, as evident by higher activities of superoxide dismutase, catalase, glutathione peroxidase and level of total antioxidant capacity, as well as lower levels of malondialdehyde in serums and livers compared to the d-galactose-treated group.
26686161	4	6	from	activities	942:951	arg1	serums					1098:1103	serums	1098:1103	serums	1098:1103	Furthermore, it was demonstrated that PHMPs had a significant protective effect against oxidative stress induced by d-galactose in mice, as evident by higher activities of superoxide dismutase, catalase, glutathione peroxidase and level of total antioxidant capacity, as well as lower levels of malondialdehyde in serums and livers compared to the d-galactose-treated group.
26686161	4	7	theme	superoxide	956:965	arg1	dismutase					967:975	superoxide dismutase	956:975	superoxide dismutase	956:975	Furthermore, it was demonstrated that PHMPs had a significant protective effect against oxidative stress induced by d-galactose in mice, as evident by higher activities of superoxide dismutase, catalase, glutathione peroxidase and level of total antioxidant capacity, as well as lower levels of malondialdehyde in serums and livers compared to the d-galactose-treated group.
26686161	5	8	theme	natural	1225:1231	arg1	antioxidant					1233:1243	promising natural antioxidant	1215:1243	promising natural antioxidant	1215:1243	These results suggested that PHMPs could be explored as promising natural antioxidant.
26686161	5	8	theme	natural	1225:1231	arg1	PHMPs					1188:1192	PHMPs	1188:1192	PHMPs	1188:1192	These results suggested that PHMPs could be explored as promising natural antioxidant.
26686161	3	9	theme	high	517:520	arg1	HPLC					557:560	HPLC	557:560	HPLC	557:560	The analysis of monosaccharide composition by high performance liquid chromatography (HPLC) revealed that PHMPs was composed of mannose, ribose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose and arabinose in molar ratio of 46.07:0.59:2.25:1.29:1.42:18.82:26.17:1.13:2.26, respectively.
26686161	3	9	theme	high	517:520	arg1	chromatography					541:554	high performance liquid chromatography	517:554	high performance liquid chromatography (HPLC)	517:561	The analysis of monosaccharide composition by high performance liquid chromatography (HPLC) revealed that PHMPs was composed of mannose, ribose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose and arabinose in molar ratio of 46.07:0.59:2.25:1.29:1.42:18.82:26.17:1.13:2.26, respectively.
26686161	3	10	from	acid	656:659	arg1	ratio					712:716	molar ratio	706:716	molar ratio of 46.07:0.59:2.25:1.29:1.42:18.82:26.17:1.13:2.26, respectively	706:781	The analysis of monosaccharide composition by high performance liquid chromatography (HPLC) revealed that PHMPs was composed of mannose, ribose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose and arabinose in molar ratio of 46.07:0.59:2.25:1.29:1.42:18.82:26.17:1.13:2.26, respectively.
26686161	4	11	theme	glutathione	988:998	arg1	peroxidase					1000:1009	glutathione peroxidase	988:1009	glutathione peroxidase	988:1009	Furthermore, it was demonstrated that PHMPs had a significant protective effect against oxidative stress induced by d-galactose in mice, as evident by higher activities of superoxide dismutase, catalase, glutathione peroxidase and level of total antioxidant capacity, as well as lower levels of malondialdehyde in serums and livers compared to the d-galactose-treated group.
26686161	2	12	dep	follows	368:374	arg1	ratio					435:439	ratio	435:439	ratio of water to material 43 mL/g	435:468	As results, the optimal parameters for extraction of PHMPs were obtained by a Box-Behnken design as follows: extraction temperature 92 °C, extracting time 190 min and ratio of water to material 43 mL/g.
26686161	2	12	dep	follows	368:374	arg1	min					427:429	extracting time 190 min	407:429	extracting time 190 min	407:429	As results, the optimal parameters for extraction of PHMPs were obtained by a Box-Behnken design as follows: extraction temperature 92 °C, extracting time 190 min and ratio of water to material 43 mL/g.
26686161	2	12	dep	follows	368:374	arg1	°C					403:404	extraction temperature 92 °C	377:404	extraction temperature 92 °C	377:404	As results, the optimal parameters for extraction of PHMPs were obtained by a Box-Behnken design as follows: extraction temperature 92 °C, extracting time 190 min and ratio of water to material 43 mL/g.
26686161	4	13	theme	d-galactose-treated	1132:1150	arg1	group					1152:1156	the d-galactose-treated group	1128:1156	the d-galactose-treated group	1128:1156	Furthermore, it was demonstrated that PHMPs had a significant protective effect against oxidative stress induced by d-galactose in mice, as evident by higher activities of superoxide dismutase, catalase, glutathione peroxidase and level of total antioxidant capacity, as well as lower levels of malondialdehyde in serums and livers compared to the d-galactose-treated group.
26686161	4	14	contain	had	828:830	arg2	effect					857:862	a significant protective effect	832:862	a significant protective effect	832:862	Furthermore, it was demonstrated that PHMPs had a significant protective effect against oxidative stress induced by d-galactose in mice, as evident by higher activities of superoxide dismutase, catalase, glutathione peroxidase and level of total antioxidant capacity, as well as lower levels of malondialdehyde in serums and livers compared to the d-galactose-treated group.
26686161	4	14	contain	had	828:830	arg1	PHMPs					822:826	PHMPs	822:826	PHMPs	822:826	Furthermore, it was demonstrated that PHMPs had a significant protective effect against oxidative stress induced by d-galactose in mice, as evident by higher activities of superoxide dismutase, catalase, glutathione peroxidase and level of total antioxidant capacity, as well as lower levels of malondialdehyde in serums and livers compared to the d-galactose-treated group.
26686161	3	15	from	acid	637:640	arg1	ratio					712:716	molar ratio	706:716	molar ratio of 46.07:0.59:2.25:1.29:1.42:18.82:26.17:1.13:2.26, respectively	706:781	The analysis of monosaccharide composition by high performance liquid chromatography (HPLC) revealed that PHMPs was composed of mannose, ribose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose and arabinose in molar ratio of 46.07:0.59:2.25:1.29:1.42:18.82:26.17:1.13:2.26, respectively.
26686161	3	16	theme	molar	706:710	arg1	ratio					712:716	molar ratio	706:716	molar ratio of 46.07:0.59:2.25:1.29:1.42:18.82:26.17:1.13:2.26, respectively	706:781	The analysis of monosaccharide composition by high performance liquid chromatography (HPLC) revealed that PHMPs was composed of mannose, ribose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose and arabinose in molar ratio of 46.07:0.59:2.25:1.29:1.42:18.82:26.17:1.13:2.26, respectively.
26686161	1	17	from	activity	174:181	arg1	PHMPs					242:246	PHMPs	242:246	PHMPs	242:246	Optimization of extraction, characterization and antioxidant activity of mycelial polysaccharides from Paecilomyces hepiali HN1 (PHMPs) were investigated.
26686161	1	17	from	activity	174:181	arg1	HN1					237:239	Paecilomyces hepiali HN1	216:239	Paecilomyces hepiali HN1 (PHMPs)	216:247	Optimization of extraction, characterization and antioxidant activity of mycelial polysaccharides from Paecilomyces hepiali HN1 (PHMPs) were investigated.
26686161	3	18	theme	liquid	534:539	arg1	HPLC					557:560	HPLC	557:560	HPLC	557:560	The analysis of monosaccharide composition by high performance liquid chromatography (HPLC) revealed that PHMPs was composed of mannose, ribose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose and arabinose in molar ratio of 46.07:0.59:2.25:1.29:1.42:18.82:26.17:1.13:2.26, respectively.
26686161	3	18	theme	liquid	534:539	arg1	chromatography					541:554	high performance liquid chromatography	517:554	high performance liquid chromatography (HPLC)	517:561	The analysis of monosaccharide composition by high performance liquid chromatography (HPLC) revealed that PHMPs was composed of mannose, ribose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose and arabinose in molar ratio of 46.07:0.59:2.25:1.29:1.42:18.82:26.17:1.13:2.26, respectively.
26686161	3	19	from	ribose	608:613	arg1	ratio					712:716	molar ratio	706:716	molar ratio of 46.07:0.59:2.25:1.29:1.42:18.82:26.17:1.13:2.26, respectively	706:781	The analysis of monosaccharide composition by high performance liquid chromatography (HPLC) revealed that PHMPs was composed of mannose, ribose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose and arabinose in molar ratio of 46.07:0.59:2.25:1.29:1.42:18.82:26.17:1.13:2.26, respectively.
26686161	1	20	theme	polysaccharides	195:209	arg1	characterization					141:156	characterization	141:156	characterization	141:156	Optimization of extraction, characterization and antioxidant activity of mycelial polysaccharides from Paecilomyces hepiali HN1 (PHMPs) were investigated.
26686161	1	20	theme	polysaccharides	195:209	arg1	activity					174:181	antioxidant activity	162:181	antioxidant activity	162:181	Optimization of extraction, characterization and antioxidant activity of mycelial polysaccharides from Paecilomyces hepiali HN1 (PHMPs) were investigated.
26686161	1	20	theme	polysaccharides	195:209	arg1	extraction					129:138	extraction	129:138	extraction	129:138	Optimization of extraction, characterization and antioxidant activity of mycelial polysaccharides from Paecilomyces hepiali HN1 (PHMPs) were investigated.
26686161	3	21	from	xylose	682:687	arg1	ratio					712:716	molar ratio	706:716	molar ratio of 46.07:0.59:2.25:1.29:1.42:18.82:26.17:1.13:2.26, respectively	706:781	The analysis of monosaccharide composition by high performance liquid chromatography (HPLC) revealed that PHMPs was composed of mannose, ribose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose and arabinose in molar ratio of 46.07:0.59:2.25:1.29:1.42:18.82:26.17:1.13:2.26, respectively.
26686161	4	22	theme	protective	846:855	arg1	effect					857:862	a significant protective effect	832:862	a significant protective effect	832:862	Furthermore, it was demonstrated that PHMPs had a significant protective effect against oxidative stress induced by d-galactose in mice, as evident by higher activities of superoxide dismutase, catalase, glutathione peroxidase and level of total antioxidant capacity, as well as lower levels of malondialdehyde in serums and livers compared to the d-galactose-treated group.
26686161	1	23	from	characterization	141:156	arg1	PHMPs					242:246	PHMPs	242:246	PHMPs	242:246	Optimization of extraction, characterization and antioxidant activity of mycelial polysaccharides from Paecilomyces hepiali HN1 (PHMPs) were investigated.
26686161	1	23	from	characterization	141:156	arg1	HN1					237:239	Paecilomyces hepiali HN1	216:239	Paecilomyces hepiali HN1 (PHMPs)	216:247	Optimization of extraction, characterization and antioxidant activity of mycelial polysaccharides from Paecilomyces hepiali HN1 (PHMPs) were investigated.
26686161	0	24	dep	HN1	108:110	arg1	characterization					12:27	characterization	12:27	characterization	12:27	Extraction, characterization and antioxidant activity of mycelial polysaccharides from Paecilomyces hepiali HN1.
26686161	0	24	dep	HN1	108:110	arg1	activity					45:52	antioxidant activity	33:52	antioxidant activity	33:52	Extraction, characterization and antioxidant activity of mycelial polysaccharides from Paecilomyces hepiali HN1.
26686161	0	24	dep	HN1	108:110	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction, characterization and antioxidant activity of mycelial polysaccharides from Paecilomyces hepiali HN1.
26686161	4	25	theme	capacity	1042:1049	arg1	dismutase					967:975	superoxide dismutase	956:975	superoxide dismutase	956:975	Furthermore, it was demonstrated that PHMPs had a significant protective effect against oxidative stress induced by d-galactose in mice, as evident by higher activities of superoxide dismutase, catalase, glutathione peroxidase and level of total antioxidant capacity, as well as lower levels of malondialdehyde in serums and livers compared to the d-galactose-treated group.
26686161	4	25	theme	capacity	1042:1049	arg1	level					1015:1019	level	1015:1019	level of total antioxidant capacity	1015:1049	Furthermore, it was demonstrated that PHMPs had a significant protective effect against oxidative stress induced by d-galactose in mice, as evident by higher activities of superoxide dismutase, catalase, glutathione peroxidase and level of total antioxidant capacity, as well as lower levels of malondialdehyde in serums and livers compared to the d-galactose-treated group.
26686161	4	25	theme	capacity	1042:1049	arg1	catalase					978:985	catalase	978:985	catalase	978:985	Furthermore, it was demonstrated that PHMPs had a significant protective effect against oxidative stress induced by d-galactose in mice, as evident by higher activities of superoxide dismutase, catalase, glutathione peroxidase and level of total antioxidant capacity, as well as lower levels of malondialdehyde in serums and livers compared to the d-galactose-treated group.
26686161	4	25	theme	capacity	1042:1049	arg1	peroxidase					1000:1009	glutathione peroxidase	988:1009	glutathione peroxidase	988:1009	Furthermore, it was demonstrated that PHMPs had a significant protective effect against oxidative stress induced by d-galactose in mice, as evident by higher activities of superoxide dismutase, catalase, glutathione peroxidase and level of total antioxidant capacity, as well as lower levels of malondialdehyde in serums and livers compared to the d-galactose-treated group.
26686161	3	26	theme	performance	522:532	arg1	HPLC					557:560	HPLC	557:560	HPLC	557:560	The analysis of monosaccharide composition by high performance liquid chromatography (HPLC) revealed that PHMPs was composed of mannose, ribose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose and arabinose in molar ratio of 46.07:0.59:2.25:1.29:1.42:18.82:26.17:1.13:2.26, respectively.
26686161	3	26	theme	performance	522:532	arg1	chromatography					541:554	high performance liquid chromatography	517:554	high performance liquid chromatography (HPLC)	517:561	The analysis of monosaccharide composition by high performance liquid chromatography (HPLC) revealed that PHMPs was composed of mannose, ribose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose and arabinose in molar ratio of 46.07:0.59:2.25:1.29:1.42:18.82:26.17:1.13:2.26, respectively.
26686161	4	27	theme	oxidative	872:880	arg1	stress					882:887	oxidative stress	872:887	oxidative stress induced by d-galactose in mice	872:918	Furthermore, it was demonstrated that PHMPs had a significant protective effect against oxidative stress induced by d-galactose in mice, as evident by higher activities of superoxide dismutase, catalase, glutathione peroxidase and level of total antioxidant capacity, as well as lower levels of malondialdehyde in serums and livers compared to the d-galactose-treated group.
26686161	3	28	from	rhamnose	616:623	arg1	ratio					712:716	molar ratio	706:716	molar ratio of 46.07:0.59:2.25:1.29:1.42:18.82:26.17:1.13:2.26, respectively	706:781	The analysis of monosaccharide composition by high performance liquid chromatography (HPLC) revealed that PHMPs was composed of mannose, ribose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose and arabinose in molar ratio of 46.07:0.59:2.25:1.29:1.42:18.82:26.17:1.13:2.26, respectively.
26686161	1	29	theme	Paecilomyces	216:227	arg1	PHMPs					242:246	PHMPs	242:246	PHMPs	242:246	Optimization of extraction, characterization and antioxidant activity of mycelial polysaccharides from Paecilomyces hepiali HN1 (PHMPs) were investigated.
26686161	1	29	theme	Paecilomyces	216:227	arg1	HN1					237:239	Paecilomyces hepiali HN1	216:239	Paecilomyces hepiali HN1 (PHMPs)	216:247	Optimization of extraction, characterization and antioxidant activity of mycelial polysaccharides from Paecilomyces hepiali HN1 (PHMPs) were investigated.
26686161	2	30	theme	water	444:448	arg1	ratio					435:439	ratio	435:439	ratio of water to material 43 mL/g	435:468	As results, the optimal parameters for extraction of PHMPs were obtained by a Box-Behnken design as follows: extraction temperature 92 °C, extracting time 190 min and ratio of water to material 43 mL/g.
26686161	2	30	theme	water	444:448	arg1	min					427:429	extracting time 190 min	407:429	extracting time 190 min	407:429	As results, the optimal parameters for extraction of PHMPs were obtained by a Box-Behnken design as follows: extraction temperature 92 °C, extracting time 190 min and ratio of water to material 43 mL/g.
26686161	2	30	theme	water	444:448	arg1	°C					403:404	extraction temperature 92 °C	377:404	extraction temperature 92 °C	377:404	As results, the optimal parameters for extraction of PHMPs were obtained by a Box-Behnken design as follows: extraction temperature 92 °C, extracting time 190 min and ratio of water to material 43 mL/g.
26686161	3	31	theme	46.07:0.59:2.25:1.29:1.42:18.82:26.17:1.13:2.26	721:767	arg1	ratio					712:716	molar ratio	706:716	molar ratio of 46.07:0.59:2.25:1.29:1.42:18.82:26.17:1.13:2.26, respectively	706:781	The analysis of monosaccharide composition by high performance liquid chromatography (HPLC) revealed that PHMPs was composed of mannose, ribose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose and arabinose in molar ratio of 46.07:0.59:2.25:1.29:1.42:18.82:26.17:1.13:2.26, respectively.
26686161	1	32	from	extraction	129:138	arg1	PHMPs					242:246	PHMPs	242:246	PHMPs	242:246	Optimization of extraction, characterization and antioxidant activity of mycelial polysaccharides from Paecilomyces hepiali HN1 (PHMPs) were investigated.
26686161	1	32	from	extraction	129:138	arg1	HN1					237:239	Paecilomyces hepiali HN1	216:239	Paecilomyces hepiali HN1 (PHMPs)	216:247	Optimization of extraction, characterization and antioxidant activity of mycelial polysaccharides from Paecilomyces hepiali HN1 (PHMPs) were investigated.
26686161	4	33	theme	antioxidant	1030:1040	arg1	capacity					1042:1049	total antioxidant capacity	1024:1049	total antioxidant capacity	1024:1049	Furthermore, it was demonstrated that PHMPs had a significant protective effect against oxidative stress induced by d-galactose in mice, as evident by higher activities of superoxide dismutase, catalase, glutathione peroxidase and level of total antioxidant capacity, as well as lower levels of malondialdehyde in serums and livers compared to the d-galactose-treated group.
26686161	1	34	theme	hepiali	229:235	arg1	PHMPs					242:246	PHMPs	242:246	PHMPs	242:246	Optimization of extraction, characterization and antioxidant activity of mycelial polysaccharides from Paecilomyces hepiali HN1 (PHMPs) were investigated.
26686161	1	34	theme	hepiali	229:235	arg1	HN1					237:239	Paecilomyces hepiali HN1	216:239	Paecilomyces hepiali HN1 (PHMPs)	216:247	Optimization of extraction, characterization and antioxidant activity of mycelial polysaccharides from Paecilomyces hepiali HN1 (PHMPs) were investigated.
26686161	4	35	theme	dismutase	967:975	arg1	levels					1069:1074	lower levels	1063:1074	lower levels of malondialdehyde in serums and livers compared to the d-galactose-treated group	1063:1156	Furthermore, it was demonstrated that PHMPs had a significant protective effect against oxidative stress induced by d-galactose in mice, as evident by higher activities of superoxide dismutase, catalase, glutathione peroxidase and level of total antioxidant capacity, as well as lower levels of malondialdehyde in serums and livers compared to the d-galactose-treated group.
26686161	4	35	theme	dismutase	967:975	arg1	activities					942:951	higher activities	935:951	higher activities of superoxide dismutase, catalase, glutathione peroxidase and level of total antioxidant capacity	935:1049	Furthermore, it was demonstrated that PHMPs had a significant protective effect against oxidative stress induced by d-galactose in mice, as evident by higher activities of superoxide dismutase, catalase, glutathione peroxidase and level of total antioxidant capacity, as well as lower levels of malondialdehyde in serums and livers compared to the d-galactose-treated group.
26686161	0	36	from	hepiali	100:106	arg1	characterization					12:27	characterization	12:27	characterization	12:27	Extraction, characterization and antioxidant activity of mycelial polysaccharides from Paecilomyces hepiali HN1.
26686161	0	36	from	hepiali	100:106	arg1	activity					45:52	antioxidant activity	33:52	antioxidant activity	33:52	Extraction, characterization and antioxidant activity of mycelial polysaccharides from Paecilomyces hepiali HN1.
26686161	0	36	from	hepiali	100:106	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction, characterization and antioxidant activity of mycelial polysaccharides from Paecilomyces hepiali HN1.
26686161	2	37	theme	PHMPs	321:325	arg1	extraction					307:316	extraction	307:316	extraction of PHMPs	307:325	As results, the optimal parameters for extraction of PHMPs were obtained by a Box-Behnken design as follows: extraction temperature 92 °C, extracting time 190 min and ratio of water to material 43 mL/g.
26686161	1	38	theme	antioxidant	162:172	arg1	activity					174:181	antioxidant activity	162:181	antioxidant activity	162:181	Optimization of extraction, characterization and antioxidant activity of mycelial polysaccharides from Paecilomyces hepiali HN1 (PHMPs) were investigated.
26686161	4	39	theme	level	1015:1019	arg1	levels					1069:1074	lower levels	1063:1074	lower levels of malondialdehyde in serums and livers compared to the d-galactose-treated group	1063:1156	Furthermore, it was demonstrated that PHMPs had a significant protective effect against oxidative stress induced by d-galactose in mice, as evident by higher activities of superoxide dismutase, catalase, glutathione peroxidase and level of total antioxidant capacity, as well as lower levels of malondialdehyde in serums and livers compared to the d-galactose-treated group.
26686161	4	39	theme	level	1015:1019	arg1	activities					942:951	higher activities	935:951	higher activities of superoxide dismutase, catalase, glutathione peroxidase and level of total antioxidant capacity	935:1049	Furthermore, it was demonstrated that PHMPs had a significant protective effect against oxidative stress induced by d-galactose in mice, as evident by higher activities of superoxide dismutase, catalase, glutathione peroxidase and level of total antioxidant capacity, as well as lower levels of malondialdehyde in serums and livers compared to the d-galactose-treated group.
26686161	3	40	from	glucose	662:668	arg1	ratio					712:716	molar ratio	706:716	molar ratio of 46.07:0.59:2.25:1.29:1.42:18.82:26.17:1.13:2.26, respectively	706:781	The analysis of monosaccharide composition by high performance liquid chromatography (HPLC) revealed that PHMPs was composed of mannose, ribose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose and arabinose in molar ratio of 46.07:0.59:2.25:1.29:1.42:18.82:26.17:1.13:2.26, respectively.
26686161	4	41	theme	total	1024:1028	arg1	capacity					1042:1049	total antioxidant capacity	1024:1049	total antioxidant capacity	1024:1049	Furthermore, it was demonstrated that PHMPs had a significant protective effect against oxidative stress induced by d-galactose in mice, as evident by higher activities of superoxide dismutase, catalase, glutathione peroxidase and level of total antioxidant capacity, as well as lower levels of malondialdehyde in serums and livers compared to the d-galactose-treated group.
26686161	4	42	theme	higher	935:940	arg1	activities					942:951	higher activities	935:951	higher activities of superoxide dismutase, catalase, glutathione peroxidase and level of total antioxidant capacity	935:1049	Furthermore, it was demonstrated that PHMPs had a significant protective effect against oxidative stress induced by d-galactose in mice, as evident by higher activities of superoxide dismutase, catalase, glutathione peroxidase and level of total antioxidant capacity, as well as lower levels of malondialdehyde in serums and livers compared to the d-galactose-treated group.
26686161	3	43	from	mannose	599:605	arg1	ratio					712:716	molar ratio	706:716	molar ratio of 46.07:0.59:2.25:1.29:1.42:18.82:26.17:1.13:2.26, respectively	706:781	The analysis of monosaccharide composition by high performance liquid chromatography (HPLC) revealed that PHMPs was composed of mannose, ribose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose and arabinose in molar ratio of 46.07:0.59:2.25:1.29:1.42:18.82:26.17:1.13:2.26, respectively.
26686161	4	44	from	livers	1109:1114	arg1	levels					1069:1074	lower levels	1063:1074	lower levels of malondialdehyde in serums and livers compared to the d-galactose-treated group	1063:1156	Furthermore, it was demonstrated that PHMPs had a significant protective effect against oxidative stress induced by d-galactose in mice, as evident by higher activities of superoxide dismutase, catalase, glutathione peroxidase and level of total antioxidant capacity, as well as lower levels of malondialdehyde in serums and livers compared to the d-galactose-treated group.
26686161	4	44	from	livers	1109:1114	arg1	activities					942:951	higher activities	935:951	higher activities of superoxide dismutase, catalase, glutathione peroxidase and level of total antioxidant capacity	935:1049	Furthermore, it was demonstrated that PHMPs had a significant protective effect against oxidative stress induced by d-galactose in mice, as evident by higher activities of superoxide dismutase, catalase, glutathione peroxidase and level of total antioxidant capacity, as well as lower levels of malondialdehyde in serums and livers compared to the d-galactose-treated group.
26686161	0	45	theme	antioxidant	33:43	arg1	activity					45:52	antioxidant activity	33:52	antioxidant activity	33:52	Extraction, characterization and antioxidant activity of mycelial polysaccharides from Paecilomyces hepiali HN1.
26686161	3	46	from	galactose	671:679	arg1	ratio					712:716	molar ratio	706:716	molar ratio of 46.07:0.59:2.25:1.29:1.42:18.82:26.17:1.13:2.26, respectively	706:781	The analysis of monosaccharide composition by high performance liquid chromatography (HPLC) revealed that PHMPs was composed of mannose, ribose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose and arabinose in molar ratio of 46.07:0.59:2.25:1.29:1.42:18.82:26.17:1.13:2.26, respectively.
26686161	2	47	theme	time	418:421	arg1	min					427:429	extracting time 190 min	407:429	extracting time 190 min	407:429	As results, the optimal parameters for extraction of PHMPs were obtained by a Box-Behnken design as follows: extraction temperature 92 °C, extracting time 190 min and ratio of water to material 43 mL/g.
26686161	0	48	theme	mycelial	57:64	arg1	polysaccharides					66:80	mycelial polysaccharides	57:80	mycelial polysaccharides	57:80	Extraction, characterization and antioxidant activity of mycelial polysaccharides from Paecilomyces hepiali HN1.
26686161	4	49	from	levels	1069:1074	arg1	livers					1109:1114	livers	1109:1114	livers	1109:1114	Furthermore, it was demonstrated that PHMPs had a significant protective effect against oxidative stress induced by d-galactose in mice, as evident by higher activities of superoxide dismutase, catalase, glutathione peroxidase and level of total antioxidant capacity, as well as lower levels of malondialdehyde in serums and livers compared to the d-galactose-treated group.
26686161	4	49	from	levels	1069:1074	arg1	serums					1098:1103	serums	1098:1103	serums	1098:1103	Furthermore, it was demonstrated that PHMPs had a significant protective effect against oxidative stress induced by d-galactose in mice, as evident by higher activities of superoxide dismutase, catalase, glutathione peroxidase and level of total antioxidant capacity, as well as lower levels of malondialdehyde in serums and livers compared to the d-galactose-treated group.
26686161	3	50	theme	glucuronic	626:635	arg1	acid					637:640	glucuronic acid	626:640	glucuronic acid	626:640	The analysis of monosaccharide composition by high performance liquid chromatography (HPLC) revealed that PHMPs was composed of mannose, ribose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose and arabinose in molar ratio of 46.07:0.59:2.25:1.29:1.42:18.82:26.17:1.13:2.26, respectively.
26686161	4	51	theme	significant	834:844	arg1	effect					857:862	a significant protective effect	832:862	a significant protective effect	832:862	Furthermore, it was demonstrated that PHMPs had a significant protective effect against oxidative stress induced by d-galactose in mice, as evident by higher activities of superoxide dismutase, catalase, glutathione peroxidase and level of total antioxidant capacity, as well as lower levels of malondialdehyde in serums and livers compared to the d-galactose-treated group.
26686161	1	52	theme	extraction	129:138	arg1	Optimization					113:124	Optimization	113:124	Optimization of extraction, characterization and antioxidant activity of mycelial polysaccharides from Paecilomyces hepiali HN1 (PHMPs)	113:247	Optimization of extraction, characterization and antioxidant activity of mycelial polysaccharides from Paecilomyces hepiali HN1 (PHMPs) were investigated.
26686161	2	53	theme	material	453:460	arg1	mL/g					465:468	material 43 mL/g	453:468	material 43 mL/g	453:468	As results, the optimal parameters for extraction of PHMPs were obtained by a Box-Behnken design as follows: extraction temperature 92 °C, extracting time 190 min and ratio of water to material 43 mL/g.
26686161	2	54	theme	extracting	407:416	arg1	min					427:429	extracting time 190 min	407:429	extracting time 190 min	407:429	As results, the optimal parameters for extraction of PHMPs were obtained by a Box-Behnken design as follows: extraction temperature 92 °C, extracting time 190 min and ratio of water to material 43 mL/g.
26686161	4	55	theme	catalase	978:985	arg1	levels					1069:1074	lower levels	1063:1074	lower levels of malondialdehyde in serums and livers compared to the d-galactose-treated group	1063:1156	Furthermore, it was demonstrated that PHMPs had a significant protective effect against oxidative stress induced by d-galactose in mice, as evident by higher activities of superoxide dismutase, catalase, glutathione peroxidase and level of total antioxidant capacity, as well as lower levels of malondialdehyde in serums and livers compared to the d-galactose-treated group.
26686161	4	55	theme	catalase	978:985	arg1	activities					942:951	higher activities	935:951	higher activities of superoxide dismutase, catalase, glutathione peroxidase and level of total antioxidant capacity	935:1049	Furthermore, it was demonstrated that PHMPs had a significant protective effect against oxidative stress induced by d-galactose in mice, as evident by higher activities of superoxide dismutase, catalase, glutathione peroxidase and level of total antioxidant capacity, as well as lower levels of malondialdehyde in serums and livers compared to the d-galactose-treated group.
26686161	1	56	from	HN1	237:239	arg1	characterization					141:156	characterization	141:156	characterization	141:156	Optimization of extraction, characterization and antioxidant activity of mycelial polysaccharides from Paecilomyces hepiali HN1 (PHMPs) were investigated.
26686161	1	56	from	HN1	237:239	arg1	activity					174:181	antioxidant activity	162:181	antioxidant activity	162:181	Optimization of extraction, characterization and antioxidant activity of mycelial polysaccharides from Paecilomyces hepiali HN1 (PHMPs) were investigated.
26686161	1	56	from	HN1	237:239	arg1	extraction					129:138	extraction	129:138	extraction	129:138	Optimization of extraction, characterization and antioxidant activity of mycelial polysaccharides from Paecilomyces hepiali HN1 (PHMPs) were investigated.
26686161	1	56	from	HN1	237:239	arg1	polysaccharides					195:209	mycelial polysaccharides	186:209	mycelial polysaccharides from Paecilomyces hepiali HN1 (PHMPs)	186:247	Optimization of extraction, characterization and antioxidant activity of mycelial polysaccharides from Paecilomyces hepiali HN1 (PHMPs) were investigated.
26686161	3	57	theme	monosaccharide	487:500	arg1	composition					502:512	monosaccharide composition	487:512	monosaccharide composition	487:512	The analysis of monosaccharide composition by high performance liquid chromatography (HPLC) revealed that PHMPs was composed of mannose, ribose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose and arabinose in molar ratio of 46.07:0.59:2.25:1.29:1.42:18.82:26.17:1.13:2.26, respectively.
26686161	4	58	theme	malondialdehyde	1079:1093	arg1	levels					1069:1074	lower levels	1063:1074	lower levels of malondialdehyde in serums and livers compared to the d-galactose-treated group	1063:1156	Furthermore, it was demonstrated that PHMPs had a significant protective effect against oxidative stress induced by d-galactose in mice, as evident by higher activities of superoxide dismutase, catalase, glutathione peroxidase and level of total antioxidant capacity, as well as lower levels of malondialdehyde in serums and livers compared to the d-galactose-treated group.
26686161	4	58	theme	malondialdehyde	1079:1093	arg1	activities					942:951	higher activities	935:951	higher activities of superoxide dismutase, catalase, glutathione peroxidase and level of total antioxidant capacity	935:1049	Furthermore, it was demonstrated that PHMPs had a significant protective effect against oxidative stress induced by d-galactose in mice, as evident by higher activities of superoxide dismutase, catalase, glutathione peroxidase and level of total antioxidant capacity, as well as lower levels of malondialdehyde in serums and livers compared to the d-galactose-treated group.
26686161	3	59	from	arabinose	693:701	arg1	ratio					712:716	molar ratio	706:716	molar ratio of 46.07:0.59:2.25:1.29:1.42:18.82:26.17:1.13:2.26, respectively	706:781	The analysis of monosaccharide composition by high performance liquid chromatography (HPLC) revealed that PHMPs was composed of mannose, ribose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose and arabinose in molar ratio of 46.07:0.59:2.25:1.29:1.42:18.82:26.17:1.13:2.26, respectively.
26686161	2	60	theme	optimal	284:290	arg1	parameters					292:301	the optimal parameters	280:301	the optimal parameters for extraction of PHMPs	280:325	As results, the optimal parameters for extraction of PHMPs were obtained by a Box-Behnken design as follows: extraction temperature 92 °C, extracting time 190 min and ratio of water to material 43 mL/g.
26686161	2	60	theme	optimal	284:290	arg1	follows					368:374	follows	368:374	follows	368:374	As results, the optimal parameters for extraction of PHMPs were obtained by a Box-Behnken design as follows: extraction temperature 92 °C, extracting time 190 min and ratio of water to material 43 mL/g.
26686161	2	60	theme	optimal	284:290	arg1	results					271:277	results	271:277	results	271:277	As results, the optimal parameters for extraction of PHMPs were obtained by a Box-Behnken design as follows: extraction temperature 92 °C, extracting time 190 min and ratio of water to material 43 mL/g.
26686161	1	61	theme	characterization	141:156	arg1	Optimization					113:124	Optimization	113:124	Optimization of extraction, characterization and antioxidant activity of mycelial polysaccharides from Paecilomyces hepiali HN1 (PHMPs)	113:247	Optimization of extraction, characterization and antioxidant activity of mycelial polysaccharides from Paecilomyces hepiali HN1 (PHMPs) were investigated.
26686161	3	62	theme	galacturonic	643:654	arg1	acid					656:659	galacturonic acid	643:659	galacturonic acid	643:659	The analysis of monosaccharide composition by high performance liquid chromatography (HPLC) revealed that PHMPs was composed of mannose, ribose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose and arabinose in molar ratio of 46.07:0.59:2.25:1.29:1.42:18.82:26.17:1.13:2.26, respectively.
26686161	1	63	theme	activity	174:181	arg1	Optimization					113:124	Optimization	113:124	Optimization of extraction, characterization and antioxidant activity of mycelial polysaccharides from Paecilomyces hepiali HN1 (PHMPs)	113:247	Optimization of extraction, characterization and antioxidant activity of mycelial polysaccharides from Paecilomyces hepiali HN1 (PHMPs) were investigated.
26686161	2	64	theme	Box-Behnken	346:356	arg1	design					358:363	a Box-Behnken design	344:363	a Box-Behnken design	344:363	As results, the optimal parameters for extraction of PHMPs were obtained by a Box-Behnken design as follows: extraction temperature 92 °C, extracting time 190 min and ratio of water to material 43 mL/g.
26686161	4	65	theme	lower	1063:1067	arg1	levels					1069:1074	lower levels	1063:1074	lower levels of malondialdehyde in serums and livers compared to the d-galactose-treated group	1063:1156	Furthermore, it was demonstrated that PHMPs had a significant protective effect against oxidative stress induced by d-galactose in mice, as evident by higher activities of superoxide dismutase, catalase, glutathione peroxidase and level of total antioxidant capacity, as well as lower levels of malondialdehyde in serums and livers compared to the d-galactose-treated group.
26686161	2	66	theme	temperature	388:398	arg1	°C					403:404	extraction temperature 92 °C	377:404	extraction temperature 92 °C	377:404	As results, the optimal parameters for extraction of PHMPs were obtained by a Box-Behnken design as follows: extraction temperature 92 °C, extracting time 190 min and ratio of water to material 43 mL/g.
26686161	0	67	theme	polysaccharides	66:80	arg1	characterization					12:27	characterization	12:27	characterization	12:27	Extraction, characterization and antioxidant activity of mycelial polysaccharides from Paecilomyces hepiali HN1.
26686161	0	67	theme	polysaccharides	66:80	arg1	activity					45:52	antioxidant activity	33:52	antioxidant activity	33:52	Extraction, characterization and antioxidant activity of mycelial polysaccharides from Paecilomyces hepiali HN1.
26686161	0	67	theme	polysaccharides	66:80	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction, characterization and antioxidant activity of mycelial polysaccharides from Paecilomyces hepiali HN1.
26686161	2	68	theme	extraction	377:386	arg1	°C					403:404	extraction temperature 92 °C	377:404	extraction temperature 92 °C	377:404	As results, the optimal parameters for extraction of PHMPs were obtained by a Box-Behnken design as follows: extraction temperature 92 °C, extracting time 190 min and ratio of water to material 43 mL/g.
26686161	5	69	theme	promising	1215:1223	arg1	antioxidant					1233:1243	promising natural antioxidant	1215:1243	promising natural antioxidant	1215:1243	These results suggested that PHMPs could be explored as promising natural antioxidant.
26686161	5	69	theme	promising	1215:1223	arg1	PHMPs					1188:1192	PHMPs	1188:1192	PHMPs	1188:1192	These results suggested that PHMPs could be explored as promising natural antioxidant.
26876826	8	0	theme	stable	1000:1005	arg1	conductivity					1018:1029	stable electrical conductivity	1000:1029	stable electrical conductivity	1000:1029	Flexural testing of conductive BC composites showed stable electrical conductivity even after repetitive bending over 100 times.
26876826	1	1	theme	silicon	164:170	arg1	SiNPs					187:191	SiNPs	187:191	SiNPs	187:191	Here we describe a unique conductive bacterial cellulose (BC) composite with silicon nanoparticles (SiNPs) and polyaniline.
26876826	1	1	theme	silicon	164:170	arg1	nanoparticles					172:184	silicon nanoparticles	164:184	silicon nanoparticles (SiNPs)	164:192	Here we describe a unique conductive bacterial cellulose (BC) composite with silicon nanoparticles (SiNPs) and polyaniline.
26876826	8	2	theme	conductive	968:977	arg1	composites					982:991	conductive BC composites	968:991	conductive BC composites	968:991	Flexural testing of conductive BC composites showed stable electrical conductivity even after repetitive bending over 100 times.
26876826	6	3	theme	acid-tagged	742:752	arg1	surface					754:760	the phosphoric acid-tagged surface	727:760	the phosphoric acid-tagged surface of the SiNPs	727:773	In addition, the phosphoric acid-tagged surface of the SiNPs enhanced the adhesion of SiNPs to the BC fibers.
26876826	2	4	theme	promising	275:283	arg1	material					291:298	a very promising anode material	268:298	a very promising anode material for Li-ion rechargeable batteries that showed a high specific capacity	268:369	BC was used as a template for binding SiNPs resulting in a very promising anode material for Li-ion rechargeable batteries that showed a high specific capacity.
26876826	4	5	theme	modified	547:554	arg1	SiNPs					556:560	the modified SiNPs	543:560	the modified SiNPs	543:560	A conformal coating of polyaniline (PANi) was formed on the modified SiNPs by in situ polymerization of aniline monomers.
26876826	1	6	with	composite	149:157	arg1	SiNPs					187:191	SiNPs	187:191	SiNPs	187:191	Here we describe a unique conductive bacterial cellulose (BC) composite with silicon nanoparticles (SiNPs) and polyaniline.
26876826	1	6	with	composite	149:157	arg1	polyaniline					198:208	polyaniline	198:208	polyaniline	198:208	Here we describe a unique conductive bacterial cellulose (BC) composite with silicon nanoparticles (SiNPs) and polyaniline.
26876826	1	6	with	composite	149:157	arg1	nanoparticles					172:184	silicon nanoparticles	164:184	silicon nanoparticles (SiNPs)	164:192	Here we describe a unique conductive bacterial cellulose (BC) composite with silicon nanoparticles (SiNPs) and polyaniline.
26876826	6	7	theme	phosphoric	731:740	arg1	surface					754:760	the phosphoric acid-tagged surface	727:760	the phosphoric acid-tagged surface of the SiNPs	727:773	In addition, the phosphoric acid-tagged surface of the SiNPs enhanced the adhesion of SiNPs to the BC fibers.
26876826	2	8	theme	Li-ion	304:309	arg1	batteries					324:332	Li-ion rechargeable batteries	304:332	Li-ion rechargeable batteries that showed a high specific capacity	304:369	BC was used as a template for binding SiNPs resulting in a very promising anode material for Li-ion rechargeable batteries that showed a high specific capacity.
26876826	1	9	theme	bacterial	124:132	arg1	composite					149:157	a unique conductive bacterial cellulose (BC) composite	104:157	a unique conductive bacterial cellulose (BC) composite with silicon nanoparticles (SiNPs) and polyaniline	104:208	Here we describe a unique conductive bacterial cellulose (BC) composite with silicon nanoparticles (SiNPs) and polyaniline.
26876826	8	10	theme	composites	982:991	arg1	testing					957:963	Flexural testing	948:963	Flexural testing of conductive BC composites	948:991	Flexural testing of conductive BC composites showed stable electrical conductivity even after repetitive bending over 100 times.
26876826	3	11	theme	phytic	417:422	arg1	acid					424:427	phytic acid	417:427	phytic acid	417:427	The surfaces of the SiNPs were modified with phytic acid to enhance the binding of aniline monomer to the surface.
26876826	0	12	theme	silicon	48:54	arg1	nanoparticles					56:68	silicon nanoparticles	48:68	silicon nanoparticles	48:68	Flexible conductive nanocellulose combined with silicon nanoparticles and polyaniline.
26876826	0	13	theme	conductive	9:18	arg1	nanocellulose					20:32	Flexible conductive nanocellulose	0:32	Flexible conductive nanocellulose	0:32	Flexible conductive nanocellulose combined with silicon nanoparticles and polyaniline.
26876826	6	14	theme	BC	813:814	arg1	fibers					816:821	the BC fibers	809:821	the BC fibers	809:821	In addition, the phosphoric acid-tagged surface of the SiNPs enhanced the adhesion of SiNPs to the BC fibers.
26876826	7	15	theme	BC	867:868	arg1	flexible					874:881	flexible	874:881	flexible	874:881	The resulting three dimensional network of BC was flexible and provided stress dissipation in the conductive BC composites.
26876826	7	15	theme	BC	867:868	arg1	network					856:862	The resulting three dimensional network	824:862	The resulting three dimensional network of BC	824:868	The resulting three dimensional network of BC was flexible and provided stress dissipation in the conductive BC composites.
26876826	3	16	mod	modified	403:410	arg1	surfaces					376:383	The surfaces	372:383	The surfaces of the SiNPs	372:396	The surfaces of the SiNPs were modified with phytic acid to enhance the binding of aniline monomer to the surface.
26876826	3	16	mod	modified	403:410	arg3	acid					424:427	phytic acid	417:427	phytic acid	417:427	The surfaces of the SiNPs were modified with phytic acid to enhance the binding of aniline monomer to the surface.
26876826	0	17	theme	Flexible	0:7	arg1	nanocellulose					20:32	Flexible conductive nanocellulose	0:32	Flexible conductive nanocellulose	0:32	Flexible conductive nanocellulose combined with silicon nanoparticles and polyaniline.
26876826	7	18	theme	dimensional	844:854	arg1	flexible					874:881	flexible	874:881	flexible	874:881	The resulting three dimensional network of BC was flexible and provided stress dissipation in the conductive BC composites.
26876826	7	18	theme	dimensional	844:854	arg1	network					856:862	The resulting three dimensional network	824:862	The resulting three dimensional network of BC	824:868	The resulting three dimensional network of BC was flexible and provided stress dissipation in the conductive BC composites.
26876826	8	19	theme	Flexural	948:955	arg1	testing					957:963	Flexural testing	948:963	Flexural testing of conductive BC composites	948:991	Flexural testing of conductive BC composites showed stable electrical conductivity even after repetitive bending over 100 times.
26876826	2	20	theme	anode	285:289	arg1	material					291:298	a very promising anode material	268:298	a very promising anode material for Li-ion rechargeable batteries that showed a high specific capacity	268:369	BC was used as a template for binding SiNPs resulting in a very promising anode material for Li-ion rechargeable batteries that showed a high specific capacity.
26876826	2	21	used	used	218:221	arg2	BC					211:212	BC	211:212	BC	211:212	BC was used as a template for binding SiNPs resulting in a very promising anode material for Li-ion rechargeable batteries that showed a high specific capacity.
26876826	2	21	used	used	218:221	arg2	template					228:235	a template	226:235	a template for binding SiNPs resulting in a very promising anode material for Li-ion rechargeable batteries that showed a high specific capacity	226:369	BC was used as a template for binding SiNPs resulting in a very promising anode material for Li-ion rechargeable batteries that showed a high specific capacity.
26876826	8	22	theme	electrical	1007:1016	arg1	conductivity					1018:1029	stable electrical conductivity	1000:1029	stable electrical conductivity	1000:1029	Flexural testing of conductive BC composites showed stable electrical conductivity even after repetitive bending over 100 times.
26876826	6	23	theme	SiNPs	800:804	arg1	adhesion					788:795	the adhesion	784:795	the adhesion of SiNPs to the BC fibers	784:821	In addition, the phosphoric acid-tagged surface of the SiNPs enhanced the adhesion of SiNPs to the BC fibers.
26876826	1	24	theme	conductive	113:122	arg1	composite					149:157	a unique conductive bacterial cellulose (BC) composite	104:157	a unique conductive bacterial cellulose (BC) composite with silicon nanoparticles (SiNPs) and polyaniline	104:208	Here we describe a unique conductive bacterial cellulose (BC) composite with silicon nanoparticles (SiNPs) and polyaniline.
26876826	3	25	theme	SiNPs	392:396	arg1	surfaces					376:383	The surfaces	372:383	The surfaces of the SiNPs	372:396	The surfaces of the SiNPs were modified with phytic acid to enhance the binding of aniline monomer to the surface.
26876826	4	26	theme	monomers	599:606	arg1	polymerization					573:586	in situ polymerization	565:586	in situ polymerization of aniline monomers	565:606	A conformal coating of polyaniline (PANi) was formed on the modified SiNPs by in situ polymerization of aniline monomers.
26876826	5	27	with	encapsulation	680:692	arg1	PANi					708:711	PANi	708:711	PANi	708:711	We also found that the phytic acid on the SiNPs was critical to ensure encapsulation of SiNPs with PANi.
26876826	8	28	theme	BC	979:980	arg1	composites					982:991	conductive BC composites	968:991	conductive BC composites	968:991	Flexural testing of conductive BC composites showed stable electrical conductivity even after repetitive bending over 100 times.
26876826	4	29	theme	aniline	591:597	arg1	monomers					599:606	aniline monomers	591:606	aniline monomers	591:606	A conformal coating of polyaniline (PANi) was formed on the modified SiNPs by in situ polymerization of aniline monomers.
26876826	4	30	theme	conformal	489:497	arg1	coating					499:505	A conformal coating	487:505	A conformal coating of polyaniline (PANi)	487:527	A conformal coating of polyaniline (PANi) was formed on the modified SiNPs by in situ polymerization of aniline monomers.
26876826	3	31	theme	monomer	463:469	arg1	binding					444:450	the binding	440:450	the binding of aniline monomer to the surface	440:484	The surfaces of the SiNPs were modified with phytic acid to enhance the binding of aniline monomer to the surface.
26876826	4	32	theme	polyaniline	510:520	arg1	coating					499:505	A conformal coating	487:505	A conformal coating of polyaniline (PANi)	487:527	A conformal coating of polyaniline (PANi) was formed on the modified SiNPs by in situ polymerization of aniline monomers.
26876826	8	33	theme	repetitive	1042:1051	arg1	bending					1053:1059	repetitive bending	1042:1059	repetitive bending	1042:1059	Flexural testing of conductive BC composites showed stable electrical conductivity even after repetitive bending over 100 times.
26876826	6	34	theme	SiNPs	769:773	arg1	surface					754:760	the phosphoric acid-tagged surface	727:760	the phosphoric acid-tagged surface of the SiNPs	727:773	In addition, the phosphoric acid-tagged surface of the SiNPs enhanced the adhesion of SiNPs to the BC fibers.
26876826	7	35	theme	conductive	922:931	arg1	composites					936:945	the conductive BC composites	918:945	the conductive BC composites	918:945	The resulting three dimensional network of BC was flexible and provided stress dissipation in the conductive BC composites.
26876826	1	36	theme	cellulose	134:142	arg1	composite					149:157	a unique conductive bacterial cellulose (BC) composite	104:157	a unique conductive bacterial cellulose (BC) composite with silicon nanoparticles (SiNPs) and polyaniline	104:208	Here we describe a unique conductive bacterial cellulose (BC) composite with silicon nanoparticles (SiNPs) and polyaniline.
26876826	7	37	theme	resulting	828:836	arg1	flexible					874:881	flexible	874:881	flexible	874:881	The resulting three dimensional network of BC was flexible and provided stress dissipation in the conductive BC composites.
26876826	7	37	theme	resulting	828:836	arg1	network					856:862	The resulting three dimensional network	824:862	The resulting three dimensional network of BC	824:868	The resulting three dimensional network of BC was flexible and provided stress dissipation in the conductive BC composites.
26876826	5	38	theme	phytic	632:637	arg1	critical					661:668	critical	661:668	critical	661:668	We also found that the phytic acid on the SiNPs was critical to ensure encapsulation of SiNPs with PANi.
26876826	5	38	theme	phytic	632:637	arg1	acid					639:642	the phytic acid	628:642	the phytic acid on the SiNPs	628:655	We also found that the phytic acid on the SiNPs was critical to ensure encapsulation of SiNPs with PANi.
26876826	4	39	dep	in	565:566	arg1	situ					568:571	situ	568:571	situ	568:571	A conformal coating of polyaniline (PANi) was formed on the modified SiNPs by in situ polymerization of aniline monomers.
26876826	7	40	theme	BC	933:934	arg1	composites					936:945	the conductive BC composites	918:945	the conductive BC composites	918:945	The resulting three dimensional network of BC was flexible and provided stress dissipation in the conductive BC composites.
26876826	2	41	theme	rechargeable	311:322	arg1	batteries					324:332	Li-ion rechargeable batteries	304:332	Li-ion rechargeable batteries that showed a high specific capacity	304:369	BC was used as a template for binding SiNPs resulting in a very promising anode material for Li-ion rechargeable batteries that showed a high specific capacity.
26876826	1	42	theme	unique	106:111	arg1	composite					149:157	a unique conductive bacterial cellulose (BC) composite	104:157	a unique conductive bacterial cellulose (BC) composite with silicon nanoparticles (SiNPs) and polyaniline	104:208	Here we describe a unique conductive bacterial cellulose (BC) composite with silicon nanoparticles (SiNPs) and polyaniline.
26876826	1	43	theme	BC	145:146	arg1	composite					149:157	a unique conductive bacterial cellulose (BC) composite	104:157	a unique conductive bacterial cellulose (BC) composite with silicon nanoparticles (SiNPs) and polyaniline	104:208	Here we describe a unique conductive bacterial cellulose (BC) composite with silicon nanoparticles (SiNPs) and polyaniline.
26876826	5	44	from	acid	639:642	arg1	SiNPs					651:655	the SiNPs	647:655	the SiNPs	647:655	We also found that the phytic acid on the SiNPs was critical to ensure encapsulation of SiNPs with PANi.
26876826	2	45	theme	specific	353:360	arg1	capacity					362:369	a high specific capacity	346:369	a high specific capacity	346:369	BC was used as a template for binding SiNPs resulting in a very promising anode material for Li-ion rechargeable batteries that showed a high specific capacity.
26876826	3	46	theme	aniline	455:461	arg1	monomer					463:469	aniline monomer	455:469	aniline monomer	455:469	The surfaces of the SiNPs were modified with phytic acid to enhance the binding of aniline monomer to the surface.
26876826	5	47	theme	SiNPs	697:701	arg1	encapsulation					680:692	encapsulation	680:692	encapsulation of SiNPs with PANi	680:711	We also found that the phytic acid on the SiNPs was critical to ensure encapsulation of SiNPs with PANi.
26876826	7	48	theme	stress	896:901	arg1	dissipation					903:913	stress dissipation	896:913	stress dissipation	896:913	The resulting three dimensional network of BC was flexible and provided stress dissipation in the conductive BC composites.
26876826	2	49	theme	high	348:351	arg1	capacity					362:369	a high specific capacity	346:369	a high specific capacity	346:369	BC was used as a template for binding SiNPs resulting in a very promising anode material for Li-ion rechargeable batteries that showed a high specific capacity.
26876826	4	50	theme	in	565:566	arg1	polymerization					573:586	in situ polymerization	565:586	in situ polymerization of aniline monomers	565:606	A conformal coating of polyaniline (PANi) was formed on the modified SiNPs by in situ polymerization of aniline monomers.
28784459	1	0	theme	racemase	312:319	arg1	alaphosphin					331:341	an alanine racemase inhibitor alaphosphin	301:341	an alanine racemase inhibitor alaphosphin	301:341	Solid-state NMR spectra of whole cells and isolated cell walls of Enterococcus faecalis grown in media containing combinations of 13C and 15N specific labels in d- and l-alanine and l-lysine (in the presence of an alanine racemase inhibitor alaphosphin) have been used to determine the composition and architecture of the cell-wall peptidoglycan.
28784459	4	1	theme	geometrical	1131:1141	arg1	architecture					1143:1154	the cell-wall chemical and geometrical architecture	1104:1154	the cell-wall chemical and geometrical architecture of whole cells of E. faecalis, a major source of nosocomial infections worldwide	1104:1235	This is the first determination of the cell-wall chemical and geometrical architecture of whole cells of E. faecalis, a major source of nosocomial infections worldwide.
28784459	0	2	theme	faecalis	80:87	arg1	peptidoglycan					50:62	the peptidoglycan	46:62	the peptidoglycan of Enterococcus faecalis	46:87	Characterization of the tertiary structure of the peptidoglycan of Enterococcus faecalis.
28784459	1	3	theme	inhibitor	321:329	arg1	alaphosphin					331:341	an alanine racemase inhibitor alaphosphin	301:341	an alanine racemase inhibitor alaphosphin	301:341	Solid-state NMR spectra of whole cells and isolated cell walls of Enterococcus faecalis grown in media containing combinations of 13C and 15N specific labels in d- and l-alanine and l-lysine (in the presence of an alanine racemase inhibitor alaphosphin) have been used to determine the composition and architecture of the cell-wall peptidoglycan.
28784459	1	4	theme	cell-wall	412:420	arg1	peptidoglycan					422:434	the cell-wall peptidoglycan	408:434	the cell-wall peptidoglycan	408:434	Solid-state NMR spectra of whole cells and isolated cell walls of Enterococcus faecalis grown in media containing combinations of 13C and 15N specific labels in d- and l-alanine and l-lysine (in the presence of an alanine racemase inhibitor alaphosphin) have been used to determine the composition and architecture of the cell-wall peptidoglycan.
28784459	3	5	theme	short-bridge	829:840	arg1	architecture					842:853	the same hybrid short-bridge architecture	813:853	the same hybrid short-bridge architecture	813:853	Connectivities of l-alanyl carbonyl‑carbon bridge labels to d-[3-13C]alanyl and l-[ε-15N]lysyl stem labels prove that the peptidoglycan of E. faecalis has the same hybrid short-bridge architecture (with a mix of parallel and perpendicular stems) as the FemA mutant of Staphylococcus aureus, in which the cross-linked stems are perpendicular to one another and the cross-linking is close to the ideal 50% value.
28784459	3	5	theme	short-bridge	829:840	arg1	mutant					916:921	the FemA mutant	907:921	the FemA mutant of Staphylococcus aureus, in which the cross-linked stems are perpendicular to one another and the cross-linking is close to the ideal 50% value	907:1066	Connectivities of l-alanyl carbonyl‑carbon bridge labels to d-[3-13C]alanyl and l-[ε-15N]lysyl stem labels prove that the peptidoglycan of E. faecalis has the same hybrid short-bridge architecture (with a mix of parallel and perpendicular stems) as the FemA mutant of Staphylococcus aureus, in which the cross-linked stems are perpendicular to one another and the cross-linking is close to the ideal 50% value.
28784459	3	6	theme	faecalis	800:807	arg1	peptidoglycan					780:792	the peptidoglycan	776:792	the peptidoglycan of E. faecalis	776:807	Connectivities of l-alanyl carbonyl‑carbon bridge labels to d-[3-13C]alanyl and l-[ε-15N]lysyl stem labels prove that the peptidoglycan of E. faecalis has the same hybrid short-bridge architecture (with a mix of parallel and perpendicular stems) as the FemA mutant of Staphylococcus aureus, in which the cross-linked stems are perpendicular to one another and the cross-linking is close to the ideal 50% value.
28784459	1	7	theme	alaphosphin	331:341	arg1	presence					289:296	the presence	285:296	the presence of an alanine racemase inhibitor alaphosphin	285:341	Solid-state NMR spectra of whole cells and isolated cell walls of Enterococcus faecalis grown in media containing combinations of 13C and 15N specific labels in d- and l-alanine and l-lysine (in the presence of an alanine racemase inhibitor alaphosphin) have been used to determine the composition and architecture of the cell-wall peptidoglycan.
28784459	1	8	theme	peptidoglycan	422:434	arg1	architecture					392:403	architecture	392:403	architecture	392:403	Solid-state NMR spectra of whole cells and isolated cell walls of Enterococcus faecalis grown in media containing combinations of 13C and 15N specific labels in d- and l-alanine and l-lysine (in the presence of an alanine racemase inhibitor alaphosphin) have been used to determine the composition and architecture of the cell-wall peptidoglycan.
28784459	1	8	theme	peptidoglycan	422:434	arg1	composition					376:386	the composition	372:386	the composition	372:386	Solid-state NMR spectra of whole cells and isolated cell walls of Enterococcus faecalis grown in media containing combinations of 13C and 15N specific labels in d- and l-alanine and l-lysine (in the presence of an alanine racemase inhibitor alaphosphin) have been used to determine the composition and architecture of the cell-wall peptidoglycan.
28784459	3	9	theme	aureus	941:946	arg1	architecture					842:853	the same hybrid short-bridge architecture	813:853	the same hybrid short-bridge architecture	813:853	Connectivities of l-alanyl carbonyl‑carbon bridge labels to d-[3-13C]alanyl and l-[ε-15N]lysyl stem labels prove that the peptidoglycan of E. faecalis has the same hybrid short-bridge architecture (with a mix of parallel and perpendicular stems) as the FemA mutant of Staphylococcus aureus, in which the cross-linked stems are perpendicular to one another and the cross-linking is close to the ideal 50% value.
28784459	3	9	theme	aureus	941:946	arg1	mutant					916:921	the FemA mutant	907:921	the FemA mutant of Staphylococcus aureus, in which the cross-linked stems are perpendicular to one another and the cross-linking is close to the ideal 50% value	907:1066	Connectivities of l-alanyl carbonyl‑carbon bridge labels to d-[3-13C]alanyl and l-[ε-15N]lysyl stem labels prove that the peptidoglycan of E. faecalis has the same hybrid short-bridge architecture (with a mix of parallel and perpendicular stems) as the FemA mutant of Staphylococcus aureus, in which the cross-linked stems are perpendicular to one another and the cross-linking is close to the ideal 50% value.
28784459	2	10	theme	stems	513:517	arg1	concentrations					477:490	the concentrations	473:490	the concentrations of (i) peptidoglycan stems without bridges, (ii) d-alanylated wall teichoic acid, (iii) cross-links, and (iv) uncross-linked tripeptide and tetra/pentapeptide stems	473:655	The compositional variables include the concentrations of (i) peptidoglycan stems without bridges, (ii) d-alanylated wall teichoic acid, (iii) cross-links, and (iv) uncross-linked tripeptide and tetra/pentapeptide stems.
28784459	4	11	theme	faecalis	1177:1184	arg1	cells					1165:1169	whole cells	1159:1169	whole cells of E. faecalis, a major source of nosocomial infections worldwide	1159:1235	This is the first determination of the cell-wall chemical and geometrical architecture of whole cells of E. faecalis, a major source of nosocomial infections worldwide.
28784459	3	12	link	cross-linked	962:973	arg1	perpendicular					985:997	perpendicular	985:997	perpendicular	985:997	Connectivities of l-alanyl carbonyl‑carbon bridge labels to d-[3-13C]alanyl and l-[ε-15N]lysyl stem labels prove that the peptidoglycan of E. faecalis has the same hybrid short-bridge architecture (with a mix of parallel and perpendicular stems) as the FemA mutant of Staphylococcus aureus, in which the cross-linked stems are perpendicular to one another and the cross-linking is close to the ideal 50% value.
28784459	3	12	link	cross-linked	962:973	arg1	stems					975:979	the cross-linked stems	958:979	the cross-linked stems	958:979	Connectivities of l-alanyl carbonyl‑carbon bridge labels to d-[3-13C]alanyl and l-[ε-15N]lysyl stem labels prove that the peptidoglycan of E. faecalis has the same hybrid short-bridge architecture (with a mix of parallel and perpendicular stems) as the FemA mutant of Staphylococcus aureus, in which the cross-linked stems are perpendicular to one another and the cross-linking is close to the ideal 50% value.
28784459	2	13	theme	peptidoglycan	499:511	arg1	stems					513:517	(i) peptidoglycan stems	495:517	(i) peptidoglycan stems without bridges, (ii) d-alanylated wall teichoic acid, (iii) cross-links, and (iv) uncross-linked tripeptide and tetra/pentapeptide stems	495:655	The compositional variables include the concentrations of (i) peptidoglycan stems without bridges, (ii) d-alanylated wall teichoic acid, (iii) cross-links, and (iv) uncross-linked tripeptide and tetra/pentapeptide stems.
28784459	3	14	theme	parallel	870:877	arg1	stems					897:901	parallel and perpendicular stems	870:901	parallel and perpendicular stems	870:901	Connectivities of l-alanyl carbonyl‑carbon bridge labels to d-[3-13C]alanyl and l-[ε-15N]lysyl stem labels prove that the peptidoglycan of E. faecalis has the same hybrid short-bridge architecture (with a mix of parallel and perpendicular stems) as the FemA mutant of Staphylococcus aureus, in which the cross-linked stems are perpendicular to one another and the cross-linking is close to the ideal 50% value.
28784459	1	15	theme	faecalis	169:176	arg1	walls					147:151	isolated cell walls	133:151	isolated cell walls of Enterococcus faecalis	133:176	Solid-state NMR spectra of whole cells and isolated cell walls of Enterococcus faecalis grown in media containing combinations of 13C and 15N specific labels in d- and l-alanine and l-lysine (in the presence of an alanine racemase inhibitor alaphosphin) have been used to determine the composition and architecture of the cell-wall peptidoglycan.
28784459	1	15	theme	faecalis	169:176	arg1	cells					123:127	whole cells	117:127	whole cells	117:127	Solid-state NMR spectra of whole cells and isolated cell walls of Enterococcus faecalis grown in media containing combinations of 13C and 15N specific labels in d- and l-alanine and l-lysine (in the presence of an alanine racemase inhibitor alaphosphin) have been used to determine the composition and architecture of the cell-wall peptidoglycan.
28784459	3	16	theme	carbonyl‑carbon	685:699	arg1	bridge					701:706	l-alanyl carbonyl‑carbon bridge	676:706	l-alanyl carbonyl‑carbon bridge	676:706	Connectivities of l-alanyl carbonyl‑carbon bridge labels to d-[3-13C]alanyl and l-[ε-15N]lysyl stem labels prove that the peptidoglycan of E. faecalis has the same hybrid short-bridge architecture (with a mix of parallel and perpendicular stems) as the FemA mutant of Staphylococcus aureus, in which the cross-linked stems are perpendicular to one another and the cross-linking is close to the ideal 50% value.
28784459	3	17	contain	has	809:811	arg2	architecture					842:853	the same hybrid short-bridge architecture	813:853	the same hybrid short-bridge architecture	813:853	Connectivities of l-alanyl carbonyl‑carbon bridge labels to d-[3-13C]alanyl and l-[ε-15N]lysyl stem labels prove that the peptidoglycan of E. faecalis has the same hybrid short-bridge architecture (with a mix of parallel and perpendicular stems) as the FemA mutant of Staphylococcus aureus, in which the cross-linked stems are perpendicular to one another and the cross-linking is close to the ideal 50% value.
28784459	3	17	contain	has	809:811	arg2	mutant					916:921	the FemA mutant	907:921	the FemA mutant of Staphylococcus aureus, in which the cross-linked stems are perpendicular to one another and the cross-linking is close to the ideal 50% value	907:1066	Connectivities of l-alanyl carbonyl‑carbon bridge labels to d-[3-13C]alanyl and l-[ε-15N]lysyl stem labels prove that the peptidoglycan of E. faecalis has the same hybrid short-bridge architecture (with a mix of parallel and perpendicular stems) as the FemA mutant of Staphylococcus aureus, in which the cross-linked stems are perpendicular to one another and the cross-linking is close to the ideal 50% value.
28784459	3	17	contain	has	809:811	arg1	peptidoglycan					780:792	the peptidoglycan	776:792	the peptidoglycan of E. faecalis	776:807	Connectivities of l-alanyl carbonyl‑carbon bridge labels to d-[3-13C]alanyl and l-[ε-15N]lysyl stem labels prove that the peptidoglycan of E. faecalis has the same hybrid short-bridge architecture (with a mix of parallel and perpendicular stems) as the FemA mutant of Staphylococcus aureus, in which the cross-linked stems are perpendicular to one another and the cross-linking is close to the ideal 50% value.
28784459	4	18	theme	architecture	1143:1154	arg1	determination					1087:1099	the first determination	1077:1099	the first determination of the cell-wall chemical and geometrical architecture of whole cells of E. faecalis, a major source of nosocomial infections worldwide	1077:1235	This is the first determination of the cell-wall chemical and geometrical architecture of whole cells of E. faecalis, a major source of nosocomial infections worldwide.
28784459	4	18	theme	architecture	1143:1154	arg1	This					1069:1072	This	1069:1072	This	1069:1072	This is the first determination of the cell-wall chemical and geometrical architecture of whole cells of E. faecalis, a major source of nosocomial infections worldwide.
28784459	2	19	dep	cross-links	580:590	arg1	iii					575:577	iii	575:577	iii	575:577	The compositional variables include the concentrations of (i) peptidoglycan stems without bridges, (ii) d-alanylated wall teichoic acid, (iii) cross-links, and (iv) uncross-linked tripeptide and tetra/pentapeptide stems.
28784459	3	20	theme	bridge	701:706	arg1	Connectivities					658:671	Connectivities	658:671	Connectivities of l-alanyl carbonyl‑carbon bridge	658:706	Connectivities of l-alanyl carbonyl‑carbon bridge labels to d-[3-13C]alanyl and l-[ε-15N]lysyl stem labels prove that the peptidoglycan of E. faecalis has the same hybrid short-bridge architecture (with a mix of parallel and perpendicular stems) as the FemA mutant of Staphylococcus aureus, in which the cross-linked stems are perpendicular to one another and the cross-linking is close to the ideal 50% value.
28784459	2	21	theme	tetra/pentapeptide	632:649	arg1	stems					651:655	tetra/pentapeptide stems	632:655	tetra/pentapeptide stems	632:655	The compositional variables include the concentrations of (i) peptidoglycan stems without bridges, (ii) d-alanylated wall teichoic acid, (iii) cross-links, and (iv) uncross-linked tripeptide and tetra/pentapeptide stems.
28784459	1	22	theme	Solid-state	90:100	arg1	spectra					106:112	Solid-state NMR spectra	90:112	Solid-state NMR spectra of whole cells and isolated cell walls of Enterococcus faecalis grown in media containing combinations of 13C and 15N specific labels in d- and l-alanine and l-lysine (in the presence of an alanine racemase inhibitor alaphosphin)	90:342	Solid-state NMR spectra of whole cells and isolated cell walls of Enterococcus faecalis grown in media containing combinations of 13C and 15N specific labels in d- and l-alanine and l-lysine (in the presence of an alanine racemase inhibitor alaphosphin) have been used to determine the composition and architecture of the cell-wall peptidoglycan.
28784459	2	23	theme	wall	554:557	arg1	acid					568:571	wall teichoic acid	554:571	wall teichoic acid	554:571	The compositional variables include the concentrations of (i) peptidoglycan stems without bridges, (ii) d-alanylated wall teichoic acid, (iii) cross-links, and (iv) uncross-linked tripeptide and tetra/pentapeptide stems.
28784459	3	24	from	aureus	941:946	arg1	perpendicular					985:997	perpendicular	985:997	perpendicular	985:997	Connectivities of l-alanyl carbonyl‑carbon bridge labels to d-[3-13C]alanyl and l-[ε-15N]lysyl stem labels prove that the peptidoglycan of E. faecalis has the same hybrid short-bridge architecture (with a mix of parallel and perpendicular stems) as the FemA mutant of Staphylococcus aureus, in which the cross-linked stems are perpendicular to one another and the cross-linking is close to the ideal 50% value.
28784459	3	24	from	aureus	941:946	arg1	stems					975:979	the cross-linked stems	958:979	the cross-linked stems	958:979	Connectivities of l-alanyl carbonyl‑carbon bridge labels to d-[3-13C]alanyl and l-[ε-15N]lysyl stem labels prove that the peptidoglycan of E. faecalis has the same hybrid short-bridge architecture (with a mix of parallel and perpendicular stems) as the FemA mutant of Staphylococcus aureus, in which the cross-linked stems are perpendicular to one another and the cross-linking is close to the ideal 50% value.
28784459	3	25	dep	alanyl	727:732	arg1	d-[3-13C					718:725	d-[3-13C	718:725	d-[3-13C	718:725	Connectivities of l-alanyl carbonyl‑carbon bridge labels to d-[3-13C]alanyl and l-[ε-15N]lysyl stem labels prove that the peptidoglycan of E. faecalis has the same hybrid short-bridge architecture (with a mix of parallel and perpendicular stems) as the FemA mutant of Staphylococcus aureus, in which the cross-linked stems are perpendicular to one another and the cross-linking is close to the ideal 50% value.
28784459	1	26	used	used	354:357	arg2	spectra					106:112	Solid-state NMR spectra	90:112	Solid-state NMR spectra of whole cells and isolated cell walls of Enterococcus faecalis grown in media containing combinations of 13C and 15N specific labels in d- and l-alanine and l-lysine (in the presence of an alanine racemase inhibitor alaphosphin)	90:342	Solid-state NMR spectra of whole cells and isolated cell walls of Enterococcus faecalis grown in media containing combinations of 13C and 15N specific labels in d- and l-alanine and l-lysine (in the presence of an alanine racemase inhibitor alaphosphin) have been used to determine the composition and architecture of the cell-wall peptidoglycan.
28784459	1	27	from	l-lysine	272:279	arg1	combinations					204:215	combinations	204:215	combinations of 13C and 15N specific labels in d- and l-alanine and l-lysine (in the presence of an alanine racemase inhibitor alaphosphin)	204:342	Solid-state NMR spectra of whole cells and isolated cell walls of Enterococcus faecalis grown in media containing combinations of 13C and 15N specific labels in d- and l-alanine and l-lysine (in the presence of an alanine racemase inhibitor alaphosphin) have been used to determine the composition and architecture of the cell-wall peptidoglycan.
28784459	1	27	from	l-lysine	272:279	arg1	presence					289:296	the presence	285:296	the presence of an alanine racemase inhibitor alaphosphin	285:341	Solid-state NMR spectra of whole cells and isolated cell walls of Enterococcus faecalis grown in media containing combinations of 13C and 15N specific labels in d- and l-alanine and l-lysine (in the presence of an alanine racemase inhibitor alaphosphin) have been used to determine the composition and architecture of the cell-wall peptidoglycan.
28784459	4	28	theme	whole	1159:1163	arg1	cells					1165:1169	whole cells	1159:1169	whole cells of E. faecalis, a major source of nosocomial infections worldwide	1159:1235	This is the first determination of the cell-wall chemical and geometrical architecture of whole cells of E. faecalis, a major source of nosocomial infections worldwide.
28784459	1	29	theme	NMR	102:104	arg1	spectra					106:112	Solid-state NMR spectra	90:112	Solid-state NMR spectra of whole cells and isolated cell walls of Enterococcus faecalis grown in media containing combinations of 13C and 15N specific labels in d- and l-alanine and l-lysine (in the presence of an alanine racemase inhibitor alaphosphin)	90:342	Solid-state NMR spectra of whole cells and isolated cell walls of Enterococcus faecalis grown in media containing combinations of 13C and 15N specific labels in d- and l-alanine and l-lysine (in the presence of an alanine racemase inhibitor alaphosphin) have been used to determine the composition and architecture of the cell-wall peptidoglycan.
28784459	3	30	theme	stems	897:901	arg1	mix					863:865	a mix	861:865	a mix of parallel and perpendicular stems	861:901	Connectivities of l-alanyl carbonyl‑carbon bridge labels to d-[3-13C]alanyl and l-[ε-15N]lysyl stem labels prove that the peptidoglycan of E. faecalis has the same hybrid short-bridge architecture (with a mix of parallel and perpendicular stems) as the FemA mutant of Staphylococcus aureus, in which the cross-linked stems are perpendicular to one another and the cross-linking is close to the ideal 50% value.
28784459	0	31	theme	tertiary	24:31	arg1	structure					33:41	the tertiary structure	20:41	the tertiary structure of the peptidoglycan of Enterococcus faecalis	20:87	Characterization of the tertiary structure of the peptidoglycan of Enterococcus faecalis.
28784459	3	32	theme	50	1058:1059	arg1	%					1060:1060	%	1060:1060	%	1060:1060	Connectivities of l-alanyl carbonyl‑carbon bridge labels to d-[3-13C]alanyl and l-[ε-15N]lysyl stem labels prove that the peptidoglycan of E. faecalis has the same hybrid short-bridge architecture (with a mix of parallel and perpendicular stems) as the FemA mutant of Staphylococcus aureus, in which the cross-linked stems are perpendicular to one another and the cross-linking is close to the ideal 50% value.
28784459	2	33	theme	uncross-linked	602:615	arg1	tripeptide					617:626	(iv) uncross-linked tripeptide	597:626	(iv) uncross-linked tripeptide	597:626	The compositional variables include the concentrations of (i) peptidoglycan stems without bridges, (ii) d-alanylated wall teichoic acid, (iii) cross-links, and (iv) uncross-linked tripeptide and tetra/pentapeptide stems.
28784459	3	34	theme	ideal	1052:1056	arg1	cross-linking					1022:1034	the cross-linking	1018:1034	the cross-linking	1018:1034	Connectivities of l-alanyl carbonyl‑carbon bridge labels to d-[3-13C]alanyl and l-[ε-15N]lysyl stem labels prove that the peptidoglycan of E. faecalis has the same hybrid short-bridge architecture (with a mix of parallel and perpendicular stems) as the FemA mutant of Staphylococcus aureus, in which the cross-linked stems are perpendicular to one another and the cross-linking is close to the ideal 50% value.
28784459	3	34	theme	ideal	1052:1056	arg1	value					1062:1066	the ideal 50% value	1048:1066	the ideal 50% value	1048:1066	Connectivities of l-alanyl carbonyl‑carbon bridge labels to d-[3-13C]alanyl and l-[ε-15N]lysyl stem labels prove that the peptidoglycan of E. faecalis has the same hybrid short-bridge architecture (with a mix of parallel and perpendicular stems) as the FemA mutant of Staphylococcus aureus, in which the cross-linked stems are perpendicular to one another and the cross-linking is close to the ideal 50% value.
28784459	3	35	dep	one	1002:1004	arg1	another					1006:1012	another	1006:1012	another	1006:1012	Connectivities of l-alanyl carbonyl‑carbon bridge labels to d-[3-13C]alanyl and l-[ε-15N]lysyl stem labels prove that the peptidoglycan of E. faecalis has the same hybrid short-bridge architecture (with a mix of parallel and perpendicular stems) as the FemA mutant of Staphylococcus aureus, in which the cross-linked stems are perpendicular to one another and the cross-linking is close to the ideal 50% value.
28784459	1	36	from	l-alanine	258:266	arg1	combinations					204:215	combinations	204:215	combinations of 13C and 15N specific labels in d- and l-alanine and l-lysine (in the presence of an alanine racemase inhibitor alaphosphin)	204:342	Solid-state NMR spectra of whole cells and isolated cell walls of Enterococcus faecalis grown in media containing combinations of 13C and 15N specific labels in d- and l-alanine and l-lysine (in the presence of an alanine racemase inhibitor alaphosphin) have been used to determine the composition and architecture of the cell-wall peptidoglycan.
28784459	1	36	from	l-alanine	258:266	arg1	presence					289:296	the presence	285:296	the presence of an alanine racemase inhibitor alaphosphin	285:341	Solid-state NMR spectra of whole cells and isolated cell walls of Enterococcus faecalis grown in media containing combinations of 13C and 15N specific labels in d- and l-alanine and l-lysine (in the presence of an alanine racemase inhibitor alaphosphin) have been used to determine the composition and architecture of the cell-wall peptidoglycan.
28784459	3	37	dep	labels	758:763	arg1	prove					765:769	prove	765:769	prove that the peptidoglycan of E. faecalis has the same hybrid short-bridge architecture (with a mix of parallel and perpendicular stems) as the FemA mutant of Staphylococcus aureus, in which the cross-linked stems are perpendicular to one another and the cross-linking is close to the ideal 50% value	765:1066	Connectivities of l-alanyl carbonyl‑carbon bridge labels to d-[3-13C]alanyl and l-[ε-15N]lysyl stem labels prove that the peptidoglycan of E. faecalis has the same hybrid short-bridge architecture (with a mix of parallel and perpendicular stems) as the FemA mutant of Staphylococcus aureus, in which the cross-linked stems are perpendicular to one another and the cross-linking is close to the ideal 50% value.
28784459	3	38	theme	%	1060:1060	arg1	cross-linking					1022:1034	the cross-linking	1018:1034	the cross-linking	1018:1034	Connectivities of l-alanyl carbonyl‑carbon bridge labels to d-[3-13C]alanyl and l-[ε-15N]lysyl stem labels prove that the peptidoglycan of E. faecalis has the same hybrid short-bridge architecture (with a mix of parallel and perpendicular stems) as the FemA mutant of Staphylococcus aureus, in which the cross-linked stems are perpendicular to one another and the cross-linking is close to the ideal 50% value.
28784459	3	38	theme	%	1060:1060	arg1	value					1062:1066	the ideal 50% value	1048:1066	the ideal 50% value	1048:1066	Connectivities of l-alanyl carbonyl‑carbon bridge labels to d-[3-13C]alanyl and l-[ε-15N]lysyl stem labels prove that the peptidoglycan of E. faecalis has the same hybrid short-bridge architecture (with a mix of parallel and perpendicular stems) as the FemA mutant of Staphylococcus aureus, in which the cross-linked stems are perpendicular to one another and the cross-linking is close to the ideal 50% value.
28784459	2	39	dep	acid	568:571	arg1	ii					537:538	ii	537:538	ii	537:538	The compositional variables include the concentrations of (i) peptidoglycan stems without bridges, (ii) d-alanylated wall teichoic acid, (iii) cross-links, and (iv) uncross-linked tripeptide and tetra/pentapeptide stems.
28784459	4	40	theme	major	1189:1193	arg1	source					1195:1200	a major source	1187:1200	a major source of nosocomial infections worldwide	1187:1235	This is the first determination of the cell-wall chemical and geometrical architecture of whole cells of E. faecalis, a major source of nosocomial infections worldwide.
28784459	4	40	theme	major	1189:1193	arg1	faecalis					1177:1184	E. faecalis	1174:1184	E. faecalis	1174:1184	This is the first determination of the cell-wall chemical and geometrical architecture of whole cells of E. faecalis, a major source of nosocomial infections worldwide.
28784459	3	41	theme	perpendicular	883:895	arg1	stems					897:901	parallel and perpendicular stems	870:901	parallel and perpendicular stems	870:901	Connectivities of l-alanyl carbonyl‑carbon bridge labels to d-[3-13C]alanyl and l-[ε-15N]lysyl stem labels prove that the peptidoglycan of E. faecalis has the same hybrid short-bridge architecture (with a mix of parallel and perpendicular stems) as the FemA mutant of Staphylococcus aureus, in which the cross-linked stems are perpendicular to one another and the cross-linking is close to the ideal 50% value.
28784459	0	42	theme	structure	33:41	arg1	Characterization					0:15	Characterization	0:15	Characterization of the tertiary structure of the peptidoglycan of Enterococcus faecalis.	0:88	Characterization of the tertiary structure of the peptidoglycan of Enterococcus faecalis.
28784459	3	43	theme	lysyl	747:751	arg1	labels					758:763	lysyl stem labels	747:763	lysyl stem labels prove that the peptidoglycan of E. faecalis has the same hybrid short-bridge architecture (with a mix of parallel and perpendicular stems) as the FemA mutant of Staphylococcus aureus, in which the cross-linked stems are perpendicular to one another and the cross-linking is close to the ideal 50% value	747:1066	Connectivities of l-alanyl carbonyl‑carbon bridge labels to d-[3-13C]alanyl and l-[ε-15N]lysyl stem labels prove that the peptidoglycan of E. faecalis has the same hybrid short-bridge architecture (with a mix of parallel and perpendicular stems) as the FemA mutant of Staphylococcus aureus, in which the cross-linked stems are perpendicular to one another and the cross-linking is close to the ideal 50% value.
28784459	1	44	from	d-	251:252	arg1	combinations					204:215	combinations	204:215	combinations of 13C and 15N specific labels in d- and l-alanine and l-lysine (in the presence of an alanine racemase inhibitor alaphosphin)	204:342	Solid-state NMR spectra of whole cells and isolated cell walls of Enterococcus faecalis grown in media containing combinations of 13C and 15N specific labels in d- and l-alanine and l-lysine (in the presence of an alanine racemase inhibitor alaphosphin) have been used to determine the composition and architecture of the cell-wall peptidoglycan.
28784459	1	44	from	d-	251:252	arg1	presence					289:296	the presence	285:296	the presence of an alanine racemase inhibitor alaphosphin	285:341	Solid-state NMR spectra of whole cells and isolated cell walls of Enterococcus faecalis grown in media containing combinations of 13C and 15N specific labels in d- and l-alanine and l-lysine (in the presence of an alanine racemase inhibitor alaphosphin) have been used to determine the composition and architecture of the cell-wall peptidoglycan.
28784459	4	45	theme	worldwide	1227:1235	arg1	source					1195:1200	a major source	1187:1200	a major source of nosocomial infections worldwide	1187:1235	This is the first determination of the cell-wall chemical and geometrical architecture of whole cells of E. faecalis, a major source of nosocomial infections worldwide.
28784459	4	45	theme	worldwide	1227:1235	arg1	faecalis					1177:1184	E. faecalis	1174:1184	E. faecalis	1174:1184	This is the first determination of the cell-wall chemical and geometrical architecture of whole cells of E. faecalis, a major source of nosocomial infections worldwide.
28784459	2	46	link	uncross-linked	602:615	arg1	tripeptide					617:626	(iv) uncross-linked tripeptide	597:626	(iv) uncross-linked tripeptide	597:626	The compositional variables include the concentrations of (i) peptidoglycan stems without bridges, (ii) d-alanylated wall teichoic acid, (iii) cross-links, and (iv) uncross-linked tripeptide and tetra/pentapeptide stems.
28784459	3	47	theme	FemA	911:914	arg1	architecture					842:853	the same hybrid short-bridge architecture	813:853	the same hybrid short-bridge architecture	813:853	Connectivities of l-alanyl carbonyl‑carbon bridge labels to d-[3-13C]alanyl and l-[ε-15N]lysyl stem labels prove that the peptidoglycan of E. faecalis has the same hybrid short-bridge architecture (with a mix of parallel and perpendicular stems) as the FemA mutant of Staphylococcus aureus, in which the cross-linked stems are perpendicular to one another and the cross-linking is close to the ideal 50% value.
28784459	3	47	theme	FemA	911:914	arg1	mutant					916:921	the FemA mutant	907:921	the FemA mutant of Staphylococcus aureus, in which the cross-linked stems are perpendicular to one another and the cross-linking is close to the ideal 50% value	907:1066	Connectivities of l-alanyl carbonyl‑carbon bridge labels to d-[3-13C]alanyl and l-[ε-15N]lysyl stem labels prove that the peptidoglycan of E. faecalis has the same hybrid short-bridge architecture (with a mix of parallel and perpendicular stems) as the FemA mutant of Staphylococcus aureus, in which the cross-linked stems are perpendicular to one another and the cross-linking is close to the ideal 50% value.
28784459	1	48	theme	13C	220:222	arg1	combinations					204:215	combinations	204:215	combinations of 13C and 15N specific labels in d- and l-alanine and l-lysine (in the presence of an alanine racemase inhibitor alaphosphin)	204:342	Solid-state NMR spectra of whole cells and isolated cell walls of Enterococcus faecalis grown in media containing combinations of 13C and 15N specific labels in d- and l-alanine and l-lysine (in the presence of an alanine racemase inhibitor alaphosphin) have been used to determine the composition and architecture of the cell-wall peptidoglycan.
28784459	0	49	theme	peptidoglycan	50:62	arg1	structure					33:41	the tertiary structure	20:41	the tertiary structure of the peptidoglycan of Enterococcus faecalis	20:87	Characterization of the tertiary structure of the peptidoglycan of Enterococcus faecalis.
28784459	3	50	theme	stem	753:756	arg1	labels					758:763	lysyl stem labels	747:763	lysyl stem labels prove that the peptidoglycan of E. faecalis has the same hybrid short-bridge architecture (with a mix of parallel and perpendicular stems) as the FemA mutant of Staphylococcus aureus, in which the cross-linked stems are perpendicular to one another and the cross-linking is close to the ideal 50% value	747:1066	Connectivities of l-alanyl carbonyl‑carbon bridge labels to d-[3-13C]alanyl and l-[ε-15N]lysyl stem labels prove that the peptidoglycan of E. faecalis has the same hybrid short-bridge architecture (with a mix of parallel and perpendicular stems) as the FemA mutant of Staphylococcus aureus, in which the cross-linked stems are perpendicular to one another and the cross-linking is close to the ideal 50% value.
28784459	2	51	theme	teichoic	559:566	arg1	acid					568:571	wall teichoic acid	554:571	wall teichoic acid	554:571	The compositional variables include the concentrations of (i) peptidoglycan stems without bridges, (ii) d-alanylated wall teichoic acid, (iii) cross-links, and (iv) uncross-linked tripeptide and tetra/pentapeptide stems.
28784459	4	52	theme	infections	1216:1225	arg1	worldwide					1227:1235	nosocomial infections worldwide	1205:1235	nosocomial infections worldwide	1205:1235	This is the first determination of the cell-wall chemical and geometrical architecture of whole cells of E. faecalis, a major source of nosocomial infections worldwide.
28784459	1	53	from	13C	220:222	arg1	d-					251:252	d-	251:252	d-	251:252	Solid-state NMR spectra of whole cells and isolated cell walls of Enterococcus faecalis grown in media containing combinations of 13C and 15N specific labels in d- and l-alanine and l-lysine (in the presence of an alanine racemase inhibitor alaphosphin) have been used to determine the composition and architecture of the cell-wall peptidoglycan.
28784459	1	53	from	13C	220:222	arg1	l-lysine					272:279	l-lysine	272:279	l-lysine (in the presence of an alanine racemase inhibitor alaphosphin)	272:342	Solid-state NMR spectra of whole cells and isolated cell walls of Enterococcus faecalis grown in media containing combinations of 13C and 15N specific labels in d- and l-alanine and l-lysine (in the presence of an alanine racemase inhibitor alaphosphin) have been used to determine the composition and architecture of the cell-wall peptidoglycan.
28784459	1	53	from	13C	220:222	arg1	l-alanine					258:266	l-alanine	258:266	l-alanine	258:266	Solid-state NMR spectra of whole cells and isolated cell walls of Enterococcus faecalis grown in media containing combinations of 13C and 15N specific labels in d- and l-alanine and l-lysine (in the presence of an alanine racemase inhibitor alaphosphin) have been used to determine the composition and architecture of the cell-wall peptidoglycan.
28784459	1	54	theme	whole	117:121	arg1	cells					123:127	whole cells	117:127	whole cells	117:127	Solid-state NMR spectra of whole cells and isolated cell walls of Enterococcus faecalis grown in media containing combinations of 13C and 15N specific labels in d- and l-alanine and l-lysine (in the presence of an alanine racemase inhibitor alaphosphin) have been used to determine the composition and architecture of the cell-wall peptidoglycan.
28784459	2	55	theme	compositional	441:453	arg1	variables					455:463	The compositional variables	437:463	The compositional variables	437:463	The compositional variables include the concentrations of (i) peptidoglycan stems without bridges, (ii) d-alanylated wall teichoic acid, (iii) cross-links, and (iv) uncross-linked tripeptide and tetra/pentapeptide stems.
28784459	4	56	theme	nosocomial	1205:1214	arg1	worldwide					1227:1235	nosocomial infections worldwide	1205:1235	nosocomial infections worldwide	1205:1235	This is the first determination of the cell-wall chemical and geometrical architecture of whole cells of E. faecalis, a major source of nosocomial infections worldwide.
28784459	1	57	from	labels	241:246	arg1	d-					251:252	d-	251:252	d-	251:252	Solid-state NMR spectra of whole cells and isolated cell walls of Enterococcus faecalis grown in media containing combinations of 13C and 15N specific labels in d- and l-alanine and l-lysine (in the presence of an alanine racemase inhibitor alaphosphin) have been used to determine the composition and architecture of the cell-wall peptidoglycan.
28784459	1	57	from	labels	241:246	arg1	l-lysine					272:279	l-lysine	272:279	l-lysine (in the presence of an alanine racemase inhibitor alaphosphin)	272:342	Solid-state NMR spectra of whole cells and isolated cell walls of Enterococcus faecalis grown in media containing combinations of 13C and 15N specific labels in d- and l-alanine and l-lysine (in the presence of an alanine racemase inhibitor alaphosphin) have been used to determine the composition and architecture of the cell-wall peptidoglycan.
28784459	1	57	from	labels	241:246	arg1	l-alanine					258:266	l-alanine	258:266	l-alanine	258:266	Solid-state NMR spectra of whole cells and isolated cell walls of Enterococcus faecalis grown in media containing combinations of 13C and 15N specific labels in d- and l-alanine and l-lysine (in the presence of an alanine racemase inhibitor alaphosphin) have been used to determine the composition and architecture of the cell-wall peptidoglycan.
28784459	4	58	theme	cells	1165:1169	arg1	architecture					1143:1154	the cell-wall chemical and geometrical architecture	1104:1154	the cell-wall chemical and geometrical architecture of whole cells of E. faecalis, a major source of nosocomial infections worldwide	1104:1235	This is the first determination of the cell-wall chemical and geometrical architecture of whole cells of E. faecalis, a major source of nosocomial infections worldwide.
28784459	1	59	theme	cells	123:127	arg1	spectra					106:112	Solid-state NMR spectra	90:112	Solid-state NMR spectra of whole cells and isolated cell walls of Enterococcus faecalis grown in media containing combinations of 13C and 15N specific labels in d- and l-alanine and l-lysine (in the presence of an alanine racemase inhibitor alaphosphin)	90:342	Solid-state NMR spectra of whole cells and isolated cell walls of Enterococcus faecalis grown in media containing combinations of 13C and 15N specific labels in d- and l-alanine and l-lysine (in the presence of an alanine racemase inhibitor alaphosphin) have been used to determine the composition and architecture of the cell-wall peptidoglycan.
28784459	3	60	theme	same	817:820	arg1	architecture					842:853	the same hybrid short-bridge architecture	813:853	the same hybrid short-bridge architecture	813:853	Connectivities of l-alanyl carbonyl‑carbon bridge labels to d-[3-13C]alanyl and l-[ε-15N]lysyl stem labels prove that the peptidoglycan of E. faecalis has the same hybrid short-bridge architecture (with a mix of parallel and perpendicular stems) as the FemA mutant of Staphylococcus aureus, in which the cross-linked stems are perpendicular to one another and the cross-linking is close to the ideal 50% value.
28784459	3	60	theme	same	817:820	arg1	mutant					916:921	the FemA mutant	907:921	the FemA mutant of Staphylococcus aureus, in which the cross-linked stems are perpendicular to one another and the cross-linking is close to the ideal 50% value	907:1066	Connectivities of l-alanyl carbonyl‑carbon bridge labels to d-[3-13C]alanyl and l-[ε-15N]lysyl stem labels prove that the peptidoglycan of E. faecalis has the same hybrid short-bridge architecture (with a mix of parallel and perpendicular stems) as the FemA mutant of Staphylococcus aureus, in which the cross-linked stems are perpendicular to one another and the cross-linking is close to the ideal 50% value.
28784459	2	61	theme	d-alanylated	541:552	arg1	acid					568:571	wall teichoic acid	554:571	wall teichoic acid	554:571	The compositional variables include the concentrations of (i) peptidoglycan stems without bridges, (ii) d-alanylated wall teichoic acid, (iii) cross-links, and (iv) uncross-linked tripeptide and tetra/pentapeptide stems.
28784459	1	62	from	combinations	204:215	arg1	d-					251:252	d-	251:252	d-	251:252	Solid-state NMR spectra of whole cells and isolated cell walls of Enterococcus faecalis grown in media containing combinations of 13C and 15N specific labels in d- and l-alanine and l-lysine (in the presence of an alanine racemase inhibitor alaphosphin) have been used to determine the composition and architecture of the cell-wall peptidoglycan.
28784459	1	62	from	combinations	204:215	arg1	l-lysine					272:279	l-lysine	272:279	l-lysine (in the presence of an alanine racemase inhibitor alaphosphin)	272:342	Solid-state NMR spectra of whole cells and isolated cell walls of Enterococcus faecalis grown in media containing combinations of 13C and 15N specific labels in d- and l-alanine and l-lysine (in the presence of an alanine racemase inhibitor alaphosphin) have been used to determine the composition and architecture of the cell-wall peptidoglycan.
28784459	1	62	from	combinations	204:215	arg1	l-alanine					258:266	l-alanine	258:266	l-alanine	258:266	Solid-state NMR spectra of whole cells and isolated cell walls of Enterococcus faecalis grown in media containing combinations of 13C and 15N specific labels in d- and l-alanine and l-lysine (in the presence of an alanine racemase inhibitor alaphosphin) have been used to determine the composition and architecture of the cell-wall peptidoglycan.
28784459	3	63	from	perpendicular	985:997	arg1	aureus					941:946	Staphylococcus aureus	926:946	Staphylococcus aureus	926:946	Connectivities of l-alanyl carbonyl‑carbon bridge labels to d-[3-13C]alanyl and l-[ε-15N]lysyl stem labels prove that the peptidoglycan of E. faecalis has the same hybrid short-bridge architecture (with a mix of parallel and perpendicular stems) as the FemA mutant of Staphylococcus aureus, in which the cross-linked stems are perpendicular to one another and the cross-linking is close to the ideal 50% value.
28784459	2	64	dep	tripeptide	617:626	arg1	iv					598:599	iv	598:599	iv	598:599	The compositional variables include the concentrations of (i) peptidoglycan stems without bridges, (ii) d-alanylated wall teichoic acid, (iii) cross-links, and (iv) uncross-linked tripeptide and tetra/pentapeptide stems.
28784459	4	65	theme	chemical	1118:1125	arg1	architecture					1143:1154	the cell-wall chemical and geometrical architecture	1104:1154	the cell-wall chemical and geometrical architecture of whole cells of E. faecalis, a major source of nosocomial infections worldwide	1104:1235	This is the first determination of the cell-wall chemical and geometrical architecture of whole cells of E. faecalis, a major source of nosocomial infections worldwide.
28784459	4	66	theme	cell-wall	1108:1116	arg1	architecture					1143:1154	the cell-wall chemical and geometrical architecture	1104:1154	the cell-wall chemical and geometrical architecture of whole cells of E. faecalis, a major source of nosocomial infections worldwide	1104:1235	This is the first determination of the cell-wall chemical and geometrical architecture of whole cells of E. faecalis, a major source of nosocomial infections worldwide.
28784459	2	67	dep	stems	513:517	arg1	i					496:496	i	496:496	i	496:496	The compositional variables include the concentrations of (i) peptidoglycan stems without bridges, (ii) d-alanylated wall teichoic acid, (iii) cross-links, and (iv) uncross-linked tripeptide and tetra/pentapeptide stems.
28784459	1	68	theme	isolated	133:140	arg1	walls					147:151	isolated cell walls	133:151	isolated cell walls of Enterococcus faecalis	133:176	Solid-state NMR spectra of whole cells and isolated cell walls of Enterococcus faecalis grown in media containing combinations of 13C and 15N specific labels in d- and l-alanine and l-lysine (in the presence of an alanine racemase inhibitor alaphosphin) have been used to determine the composition and architecture of the cell-wall peptidoglycan.
28784459	1	69	theme	15N	228:230	arg1	labels					241:246	15N specific labels	228:246	15N specific labels	228:246	Solid-state NMR spectra of whole cells and isolated cell walls of Enterococcus faecalis grown in media containing combinations of 13C and 15N specific labels in d- and l-alanine and l-lysine (in the presence of an alanine racemase inhibitor alaphosphin) have been used to determine the composition and architecture of the cell-wall peptidoglycan.
28784459	0	70	theme	Enterococcus	67:78	arg1	faecalis					80:87	Enterococcus faecalis	67:87	Enterococcus faecalis	67:87	Characterization of the tertiary structure of the peptidoglycan of Enterococcus faecalis.
28784459	1	71	contain	containing	193:202	arg2	combinations					204:215	combinations	204:215	combinations of 13C and 15N specific labels in d- and l-alanine and l-lysine (in the presence of an alanine racemase inhibitor alaphosphin)	204:342	Solid-state NMR spectra of whole cells and isolated cell walls of Enterococcus faecalis grown in media containing combinations of 13C and 15N specific labels in d- and l-alanine and l-lysine (in the presence of an alanine racemase inhibitor alaphosphin) have been used to determine the composition and architecture of the cell-wall peptidoglycan.
28784459	1	71	contain	containing	193:202	arg1	media					187:191	media	187:191	media containing combinations of 13C and 15N specific labels in d- and l-alanine and l-lysine (in the presence of an alanine racemase inhibitor alaphosphin)	187:342	Solid-state NMR spectra of whole cells and isolated cell walls of Enterococcus faecalis grown in media containing combinations of 13C and 15N specific labels in d- and l-alanine and l-lysine (in the presence of an alanine racemase inhibitor alaphosphin) have been used to determine the composition and architecture of the cell-wall peptidoglycan.
28784459	3	72	theme	cross-linked	962:973	arg1	perpendicular					985:997	perpendicular	985:997	perpendicular	985:997	Connectivities of l-alanyl carbonyl‑carbon bridge labels to d-[3-13C]alanyl and l-[ε-15N]lysyl stem labels prove that the peptidoglycan of E. faecalis has the same hybrid short-bridge architecture (with a mix of parallel and perpendicular stems) as the FemA mutant of Staphylococcus aureus, in which the cross-linked stems are perpendicular to one another and the cross-linking is close to the ideal 50% value.
28784459	3	72	theme	cross-linked	962:973	arg1	stems					975:979	the cross-linked stems	958:979	the cross-linked stems	958:979	Connectivities of l-alanyl carbonyl‑carbon bridge labels to d-[3-13C]alanyl and l-[ε-15N]lysyl stem labels prove that the peptidoglycan of E. faecalis has the same hybrid short-bridge architecture (with a mix of parallel and perpendicular stems) as the FemA mutant of Staphylococcus aureus, in which the cross-linked stems are perpendicular to one another and the cross-linking is close to the ideal 50% value.
28784459	4	73	theme	first	1081:1085	arg1	determination					1087:1099	the first determination	1077:1099	the first determination of the cell-wall chemical and geometrical architecture of whole cells of E. faecalis, a major source of nosocomial infections worldwide	1077:1235	This is the first determination of the cell-wall chemical and geometrical architecture of whole cells of E. faecalis, a major source of nosocomial infections worldwide.
28784459	4	73	theme	first	1081:1085	arg1	This					1069:1072	This	1069:1072	This	1069:1072	This is the first determination of the cell-wall chemical and geometrical architecture of whole cells of E. faecalis, a major source of nosocomial infections worldwide.
28784459	1	74	theme	cell	142:145	arg1	walls					147:151	isolated cell walls	133:151	isolated cell walls of Enterococcus faecalis	133:176	Solid-state NMR spectra of whole cells and isolated cell walls of Enterococcus faecalis grown in media containing combinations of 13C and 15N specific labels in d- and l-alanine and l-lysine (in the presence of an alanine racemase inhibitor alaphosphin) have been used to determine the composition and architecture of the cell-wall peptidoglycan.
28784459	3	75	theme	hybrid	822:827	arg1	architecture					842:853	the same hybrid short-bridge architecture	813:853	the same hybrid short-bridge architecture	813:853	Connectivities of l-alanyl carbonyl‑carbon bridge labels to d-[3-13C]alanyl and l-[ε-15N]lysyl stem labels prove that the peptidoglycan of E. faecalis has the same hybrid short-bridge architecture (with a mix of parallel and perpendicular stems) as the FemA mutant of Staphylococcus aureus, in which the cross-linked stems are perpendicular to one another and the cross-linking is close to the ideal 50% value.
28784459	3	75	theme	hybrid	822:827	arg1	mutant					916:921	the FemA mutant	907:921	the FemA mutant of Staphylococcus aureus, in which the cross-linked stems are perpendicular to one another and the cross-linking is close to the ideal 50% value	907:1066	Connectivities of l-alanyl carbonyl‑carbon bridge labels to d-[3-13C]alanyl and l-[ε-15N]lysyl stem labels prove that the peptidoglycan of E. faecalis has the same hybrid short-bridge architecture (with a mix of parallel and perpendicular stems) as the FemA mutant of Staphylococcus aureus, in which the cross-linked stems are perpendicular to one another and the cross-linking is close to the ideal 50% value.
28784459	1	76	theme	specific	232:239	arg1	labels					241:246	15N specific labels	228:246	15N specific labels	228:246	Solid-state NMR spectra of whole cells and isolated cell walls of Enterococcus faecalis grown in media containing combinations of 13C and 15N specific labels in d- and l-alanine and l-lysine (in the presence of an alanine racemase inhibitor alaphosphin) have been used to determine the composition and architecture of the cell-wall peptidoglycan.
28784459	1	77	theme	alanine	304:310	arg1	alaphosphin					331:341	an alanine racemase inhibitor alaphosphin	301:341	an alanine racemase inhibitor alaphosphin	301:341	Solid-state NMR spectra of whole cells and isolated cell walls of Enterococcus faecalis grown in media containing combinations of 13C and 15N specific labels in d- and l-alanine and l-lysine (in the presence of an alanine racemase inhibitor alaphosphin) have been used to determine the composition and architecture of the cell-wall peptidoglycan.
28784459	3	78	theme	l-alanyl	676:683	arg1	bridge					701:706	l-alanyl carbonyl‑carbon bridge	676:706	l-alanyl carbonyl‑carbon bridge	676:706	Connectivities of l-alanyl carbonyl‑carbon bridge labels to d-[3-13C]alanyl and l-[ε-15N]lysyl stem labels prove that the peptidoglycan of E. faecalis has the same hybrid short-bridge architecture (with a mix of parallel and perpendicular stems) as the FemA mutant of Staphylococcus aureus, in which the cross-linked stems are perpendicular to one another and the cross-linking is close to the ideal 50% value.
28784459	1	79	theme	walls	147:151	arg1	spectra					106:112	Solid-state NMR spectra	90:112	Solid-state NMR spectra of whole cells and isolated cell walls of Enterococcus faecalis grown in media containing combinations of 13C and 15N specific labels in d- and l-alanine and l-lysine (in the presence of an alanine racemase inhibitor alaphosphin)	90:342	Solid-state NMR spectra of whole cells and isolated cell walls of Enterococcus faecalis grown in media containing combinations of 13C and 15N specific labels in d- and l-alanine and l-lysine (in the presence of an alanine racemase inhibitor alaphosphin) have been used to determine the composition and architecture of the cell-wall peptidoglycan.
28784459	1	80	theme	labels	241:246	arg1	combinations					204:215	combinations	204:215	combinations of 13C and 15N specific labels in d- and l-alanine and l-lysine (in the presence of an alanine racemase inhibitor alaphosphin)	204:342	Solid-state NMR spectra of whole cells and isolated cell walls of Enterococcus faecalis grown in media containing combinations of 13C and 15N specific labels in d- and l-alanine and l-lysine (in the presence of an alanine racemase inhibitor alaphosphin) have been used to determine the composition and architecture of the cell-wall peptidoglycan.
26657585	0	0	theme	indica	76:81	arg1	mango					59:63	ready-to-eat mango	46:63	ready-to-eat mango (Mangifera indica L.) bars	46:90	Optimisation of gellan gum edible coating for ready-to-eat mango (Mangifera indica L.) bars.
26657585	0	0	theme	indica	76:81	arg1	L.					83:84	Mangifera indica L.	66:84	Mangifera indica L.	66:84	Optimisation of gellan gum edible coating for ready-to-eat mango (Mangifera indica L.) bars.
26657585	2	1	theme	coating	382:388	arg1	solution					390:397	the coating solution	378:397	the coating solution	378:397	The independent variables were the concentration of gellan (L/H90/10) and the concentration of Ca(2+) in the coating solution, as well as the storage time after coating application.
26657585	2	2	theme	independent	277:287	arg1	variables					289:297	The independent variables	273:297	The independent variables	273:297	The independent variables were the concentration of gellan (L/H90/10) and the concentration of Ca(2+) in the coating solution, as well as the storage time after coating application.
26657585	2	2	theme	independent	277:287	arg1	concentration					308:320	the concentration	304:320	the concentration of gellan (L/H90/10)	304:341	The independent variables were the concentration of gellan (L/H90/10) and the concentration of Ca(2+) in the coating solution, as well as the storage time after coating application.
26657585	9	3	theme	sensory	1243:1249	arg1	firmness					1283:1290	firmness	1283:1290	firmness	1283:1290	This work showed that the coating can improve mango bars sensory characteristics (appearance and firmness) and stability in terms of syneresis, colour and volatiles content during storage increasing the commercial value of the final product.
26657585	9	3	theme	sensory	1243:1249	arg1	appearance					1268:1277	appearance	1268:1277	appearance	1268:1277	This work showed that the coating can improve mango bars sensory characteristics (appearance and firmness) and stability in terms of syneresis, colour and volatiles content during storage increasing the commercial value of the final product.
26657585	9	3	theme	sensory	1243:1249	arg1	characteristics					1251:1265	mango bars sensory characteristics	1232:1265	mango bars sensory characteristics (appearance and firmness)	1232:1291	This work showed that the coating can improve mango bars sensory characteristics (appearance and firmness) and stability in terms of syneresis, colour and volatiles content during storage increasing the commercial value of the final product.
26657585	0	4	theme	Mangifera	66:74	arg1	mango					59:63	ready-to-eat mango	46:63	ready-to-eat mango (Mangifera indica L.) bars	46:90	Optimisation of gellan gum edible coating for ready-to-eat mango (Mangifera indica L.) bars.
26657585	0	4	theme	Mangifera	66:74	arg1	L.					83:84	Mangifera indica L.	66:84	Mangifera indica L.	66:84	Optimisation of gellan gum edible coating for ready-to-eat mango (Mangifera indica L.) bars.
26657585	7	5	theme	mango	949:953	arg1	bars					955:958	the mango bars	945:958	the mango bars	945:958	Considering the model predictions, 1.0%wt L/H90/10 with addition of 6 mM Ca(2+) could represent the optimal coating formulation for the mango bars.
26657585	8	6	with	release	965:971	arg1	formulation					1059:1069	the selected formulation	1046:1069	the selected formulation	1046:1069	The release of eight volatile compounds from the uncoated and coated mango bars with the selected formulation was analysed by Headspace - Solid Phase Micro Extraction-Gas Chromatography during 9 days of refrigerated storage.
26657585	1	7	theme	ready-to-eat	182:193	arg1	bars					201:204	ready-to-eat mango bars	182:204	ready-to-eat mango bars	182:204	The optimisation of an edible coating based on low acyl (L)/high acyl (H) gellan gum for ready-to-eat mango bars was performed through a central composite rotatable design (CCRD).
26657585	7	8	theme	model	829:833	arg1	predictions					835:845	the model predictions	825:845	the model predictions	825:845	Considering the model predictions, 1.0%wt L/H90/10 with addition of 6 mM Ca(2+) could represent the optimal coating formulation for the mango bars.
26657585	8	9	theme	refrigerated	1164:1175	arg1	storage					1177:1183	refrigerated storage	1164:1183	refrigerated storage	1164:1183	The release of eight volatile compounds from the uncoated and coated mango bars with the selected formulation was analysed by Headspace - Solid Phase Micro Extraction-Gas Chromatography during 9 days of refrigerated storage.
26657585	9	10	theme	mango	1232:1236	arg1	firmness					1283:1290	firmness	1283:1290	firmness	1283:1290	This work showed that the coating can improve mango bars sensory characteristics (appearance and firmness) and stability in terms of syneresis, colour and volatiles content during storage increasing the commercial value of the final product.
26657585	9	10	theme	mango	1232:1236	arg1	appearance					1268:1277	appearance	1268:1277	appearance	1268:1277	This work showed that the coating can improve mango bars sensory characteristics (appearance and firmness) and stability in terms of syneresis, colour and volatiles content during storage increasing the commercial value of the final product.
26657585	9	10	theme	mango	1232:1236	arg1	characteristics					1251:1265	mango bars sensory characteristics	1232:1265	mango bars sensory characteristics (appearance and firmness)	1232:1291	This work showed that the coating can improve mango bars sensory characteristics (appearance and firmness) and stability in terms of syneresis, colour and volatiles content during storage increasing the commercial value of the final product.
26657585	1	11	theme	mango	195:199	arg1	bars					201:204	ready-to-eat mango bars	182:204	ready-to-eat mango bars	182:204	The optimisation of an edible coating based on low acyl (L)/high acyl (H) gellan gum for ready-to-eat mango bars was performed through a central composite rotatable design (CCRD).
26657585	4	12	theme	Gellan	570:575	arg1	concentration					577:589	Gellan concentration	570:589	Gellan concentration	570:589	Gellan concentration was the independent variable that most influenced the thickness of the coating.
26657585	4	12	theme	Gellan	570:575	arg1	variable					611:618	the independent variable	595:618	the independent variable that most influenced the thickness of the coating	595:668	Gellan concentration was the independent variable that most influenced the thickness of the coating.
26657585	9	13	theme	volatiles	1341:1349	arg1	terms					1310:1314	terms	1310:1314	terms of syneresis, colour and volatiles content	1310:1357	This work showed that the coating can improve mango bars sensory characteristics (appearance and firmness) and stability in terms of syneresis, colour and volatiles content during storage increasing the commercial value of the final product.
26657585	8	14	theme	compounds	991:999	arg1	release					965:971	The release	961:971	The release of eight volatile compounds from the uncoated and coated mango bars with the selected formulation	961:1069	The release of eight volatile compounds from the uncoated and coated mango bars with the selected formulation was analysed by Headspace - Solid Phase Micro Extraction-Gas Chromatography during 9 days of refrigerated storage.
26657585	8	15	theme	Phase	1105:1109	arg1	Chromatography					1132:1145	Headspace - Solid Phase Micro Extraction-Gas Chromatography	1087:1145	Chromatography	1132:1145	The release of eight volatile compounds from the uncoated and coated mango bars with the selected formulation was analysed by Headspace - Solid Phase Micro Extraction-Gas Chromatography during 9 days of refrigerated storage.
26657585	7	16	theme	optimal	913:919	arg1	formulation					929:939	the optimal coating formulation	909:939	the optimal coating formulation for the mango bars	909:958	Considering the model predictions, 1.0%wt L/H90/10 with addition of 6 mM Ca(2+) could represent the optimal coating formulation for the mango bars.
26657585	1	17	theme	low	140:142	arg1	gum					174:176	low acyl (L)/high acyl (H) gellan gum	140:176	low acyl (L)/high acyl (H) gellan gum for ready-to-eat mango bars	140:204	The optimisation of an edible coating based on low acyl (L)/high acyl (H) gellan gum for ready-to-eat mango bars was performed through a central composite rotatable design (CCRD).
26657585	8	18	theme	volatile	982:989	arg1	compounds					991:999	eight volatile compounds	976:999	eight volatile compounds	976:999	The release of eight volatile compounds from the uncoated and coated mango bars with the selected formulation was analysed by Headspace - Solid Phase Micro Extraction-Gas Chromatography during 9 days of refrigerated storage.
26657585	9	19	dep	syneresis	1319:1327	arg1	content					1351:1357	content	1351:1357	content	1351:1357	This work showed that the coating can improve mango bars sensory characteristics (appearance and firmness) and stability in terms of syneresis, colour and volatiles content during storage increasing the commercial value of the final product.
26657585	5	20	dep	low	691:693	arg1	%					727:727	<1.64%	722:727	<1.64%	722:727	Syneresis was quite low for the conditions tested (<1.64%).
26657585	9	21	theme	colour	1330:1335	arg1	terms					1310:1314	terms	1310:1314	terms of syneresis, colour and volatiles content	1310:1357	This work showed that the coating can improve mango bars sensory characteristics (appearance and firmness) and stability in terms of syneresis, colour and volatiles content during storage increasing the commercial value of the final product.
26657585	4	22	theme	coating	662:668	arg1	thickness					645:653	the thickness	641:653	the thickness of the coating	641:668	Gellan concentration was the independent variable that most influenced the thickness of the coating.
26657585	1	23	theme	acyl	144:147	arg1	gum					174:176	low acyl (L)/high acyl (H) gellan gum	140:176	low acyl (L)/high acyl (H) gellan gum for ready-to-eat mango bars	140:204	The optimisation of an edible coating based on low acyl (L)/high acyl (H) gellan gum for ready-to-eat mango bars was performed through a central composite rotatable design (CCRD).
26657585	2	24	theme	Ca	368:369	arg1	L/H90/10					333:340	L/H90/10	333:340	L/H90/10	333:340	The independent variables were the concentration of gellan (L/H90/10) and the concentration of Ca(2+) in the coating solution, as well as the storage time after coating application.
26657585	2	24	theme	Ca	368:369	arg1	variables					289:297	The independent variables	273:297	The independent variables	273:297	The independent variables were the concentration of gellan (L/H90/10) and the concentration of Ca(2+) in the coating solution, as well as the storage time after coating application.
26657585	2	24	theme	Ca	368:369	arg1	concentration					308:320	the concentration	304:320	the concentration of gellan (L/H90/10)	304:341	The independent variables were the concentration of gellan (L/H90/10) and the concentration of Ca(2+) in the coating solution, as well as the storage time after coating application.
26657585	2	24	theme	Ca	368:369	arg1	concentration					351:363	the concentration	347:363	the concentration of Ca(2+) in the coating solution	347:397	The independent variables were the concentration of gellan (L/H90/10) and the concentration of Ca(2+) in the coating solution, as well as the storage time after coating application.
26657585	2	24	theme	Ca	368:369	arg1	time					423:426	the storage time	411:426	the storage time after coating application	411:452	The independent variables were the concentration of gellan (L/H90/10) and the concentration of Ca(2+) in the coating solution, as well as the storage time after coating application.
26657585	6	25	theme	colour	746:751	arg1	low					770:772	low	770:772	low	770:772	Similarly, the colour alterations were low during the entire storage time (ΔE<5).
26657585	6	25	theme	colour	746:751	arg1	alterations					753:763	the colour alterations	742:763	the colour alterations	742:763	Similarly, the colour alterations were low during the entire storage time (ΔE<5).
26657585	8	26	from	bars	1036:1039	arg1	release					965:971	The release	961:971	The release of eight volatile compounds from the uncoated and coated mango bars with the selected formulation	961:1069	The release of eight volatile compounds from the uncoated and coated mango bars with the selected formulation was analysed by Headspace - Solid Phase Micro Extraction-Gas Chromatography during 9 days of refrigerated storage.
26657585	9	27	theme	commercial	1389:1398	arg1	value					1400:1404	the commercial value	1385:1404	the commercial value of the final product	1385:1425	This work showed that the coating can improve mango bars sensory characteristics (appearance and firmness) and stability in terms of syneresis, colour and volatiles content during storage increasing the commercial value of the final product.
26657585	7	28	theme	Ca	886:887	arg1	addition					869:876	addition	869:876	addition of 6 mM Ca(2+)	869:891	Considering the model predictions, 1.0%wt L/H90/10 with addition of 6 mM Ca(2+) could represent the optimal coating formulation for the mango bars.
26657585	8	29	theme	selected	1050:1057	arg1	formulation					1059:1069	the selected formulation	1046:1069	the selected formulation	1046:1069	The release of eight volatile compounds from the uncoated and coated mango bars with the selected formulation was analysed by Headspace - Solid Phase Micro Extraction-Gas Chromatography during 9 days of refrigerated storage.
26657585	3	30	theme	response	459:466	arg1	thickness					503:511	the coating thickness	491:511	the coating thickness	491:511	The response variables studied were the coating thickness, mango bars firmness, syneresis, and colour alterations.
26657585	3	30	theme	response	459:466	arg1	variables					468:476	The response variables	455:476	The response variables studied	455:484	The response variables studied were the coating thickness, mango bars firmness, syneresis, and colour alterations.
26657585	2	31	theme	gellan	325:330	arg1	L/H90/10					333:340	L/H90/10	333:340	L/H90/10	333:340	The independent variables were the concentration of gellan (L/H90/10) and the concentration of Ca(2+) in the coating solution, as well as the storage time after coating application.
26657585	2	31	theme	gellan	325:330	arg1	variables					289:297	The independent variables	273:297	The independent variables	273:297	The independent variables were the concentration of gellan (L/H90/10) and the concentration of Ca(2+) in the coating solution, as well as the storage time after coating application.
26657585	2	31	theme	gellan	325:330	arg1	concentration					308:320	the concentration	304:320	the concentration of gellan (L/H90/10)	304:341	The independent variables were the concentration of gellan (L/H90/10) and the concentration of Ca(2+) in the coating solution, as well as the storage time after coating application.
26657585	2	31	theme	gellan	325:330	arg1	concentration					351:363	the concentration	347:363	the concentration of Ca(2+) in the coating solution	347:397	The independent variables were the concentration of gellan (L/H90/10) and the concentration of Ca(2+) in the coating solution, as well as the storage time after coating application.
26657585	2	31	theme	gellan	325:330	arg1	time					423:426	the storage time	411:426	the storage time after coating application	411:452	The independent variables were the concentration of gellan (L/H90/10) and the concentration of Ca(2+) in the coating solution, as well as the storage time after coating application.
26657585	1	32	theme	L	150:150	arg1	gum					174:176	low acyl (L)/high acyl (H) gellan gum	140:176	low acyl (L)/high acyl (H) gellan gum for ready-to-eat mango bars	140:204	The optimisation of an edible coating based on low acyl (L)/high acyl (H) gellan gum for ready-to-eat mango bars was performed through a central composite rotatable design (CCRD).
26657585	0	33	theme	gum	23:25	arg1	coating					34:40	gellan gum edible coating	16:40	gellan gum edible coating	16:40	Optimisation of gellan gum edible coating for ready-to-eat mango (Mangifera indica L.) bars.
26657585	2	34	theme	coating	434:440	arg1	application					442:452	coating application	434:452	coating application	434:452	The independent variables were the concentration of gellan (L/H90/10) and the concentration of Ca(2+) in the coating solution, as well as the storage time after coating application.
26657585	8	35	theme	mango	1030:1034	arg1	bars					1036:1039	the uncoated and coated mango bars	1006:1039	the uncoated and coated mango bars	1006:1039	The release of eight volatile compounds from the uncoated and coated mango bars with the selected formulation was analysed by Headspace - Solid Phase Micro Extraction-Gas Chromatography during 9 days of refrigerated storage.
26657585	9	36	theme	final	1413:1417	arg1	product					1419:1425	the final product	1409:1425	the final product	1409:1425	This work showed that the coating can improve mango bars sensory characteristics (appearance and firmness) and stability in terms of syneresis, colour and volatiles content during storage increasing the commercial value of the final product.
26657585	0	37	theme	gellan	16:21	arg1	coating					34:40	gellan gum edible coating	16:40	gellan gum edible coating	16:40	Optimisation of gellan gum edible coating for ready-to-eat mango (Mangifera indica L.) bars.
26657585	9	38	theme	syneresis	1319:1327	arg1	terms					1310:1314	terms	1310:1314	terms of syneresis, colour and volatiles content	1310:1357	This work showed that the coating can improve mango bars sensory characteristics (appearance and firmness) and stability in terms of syneresis, colour and volatiles content during storage increasing the commercial value of the final product.
26657585	6	39	theme	entire	785:790	arg1	ΔE<5					806:809	ΔE<5	806:809	ΔE<5	806:809	Similarly, the colour alterations were low during the entire storage time (ΔE<5).
26657585	6	39	theme	entire	785:790	arg1	time					800:803	the entire storage time	781:803	the entire storage time (ΔE<5)	781:810	Similarly, the colour alterations were low during the entire storage time (ΔE<5).
26657585	1	40	theme	/high	152:156	arg1	gum					174:176	low acyl (L)/high acyl (H) gellan gum	140:176	low acyl (L)/high acyl (H) gellan gum for ready-to-eat mango bars	140:204	The optimisation of an edible coating based on low acyl (L)/high acyl (H) gellan gum for ready-to-eat mango bars was performed through a central composite rotatable design (CCRD).
26657585	0	41	theme	coating	34:40	arg1	Optimisation					0:11	Optimisation	0:11	Optimisation of gellan gum edible coating for ready-to-eat mango (Mangifera indica L.) bars.	0:91	Optimisation of gellan gum edible coating for ready-to-eat mango (Mangifera indica L.) bars.
26657585	8	42	theme	Solid	1099:1103	arg1	Chromatography					1132:1145	Headspace - Solid Phase Micro Extraction-Gas Chromatography	1087:1145	Chromatography	1132:1145	The release of eight volatile compounds from the uncoated and coated mango bars with the selected formulation was analysed by Headspace - Solid Phase Micro Extraction-Gas Chromatography during 9 days of refrigerated storage.
26657585	7	43	with	L/H90/10	855:862	arg1	addition					869:876	addition	869:876	addition of 6 mM Ca(2+)	869:891	Considering the model predictions, 1.0%wt L/H90/10 with addition of 6 mM Ca(2+) could represent the optimal coating formulation for the mango bars.
26657585	1	44	theme	acyl	158:161	arg1	gum					174:176	low acyl (L)/high acyl (H) gellan gum	140:176	low acyl (L)/high acyl (H) gellan gum for ready-to-eat mango bars	140:204	The optimisation of an edible coating based on low acyl (L)/high acyl (H) gellan gum for ready-to-eat mango bars was performed through a central composite rotatable design (CCRD).
26657585	0	45	theme	edible	27:32	arg1	coating					34:40	gellan gum edible coating	16:40	gellan gum edible coating	16:40	Optimisation of gellan gum edible coating for ready-to-eat mango (Mangifera indica L.) bars.
26657585	1	46	theme	central	230:236	arg1	CCRD					266:269	CCRD	266:269	CCRD	266:269	The optimisation of an edible coating based on low acyl (L)/high acyl (H) gellan gum for ready-to-eat mango bars was performed through a central composite rotatable design (CCRD).
26657585	1	46	theme	central	230:236	arg1	design					258:263	a central composite rotatable design	228:263	a central composite rotatable design (CCRD)	228:270	The optimisation of an edible coating based on low acyl (L)/high acyl (H) gellan gum for ready-to-eat mango bars was performed through a central composite rotatable design (CCRD).
26657585	2	47	theme	storage	415:421	arg1	time					423:426	the storage time	411:426	the storage time after coating application	411:452	The independent variables were the concentration of gellan (L/H90/10) and the concentration of Ca(2+) in the coating solution, as well as the storage time after coating application.
26657585	3	48	theme	bars	520:523	arg1	firmness					525:532	mango bars firmness	514:532	mango bars firmness	514:532	The response variables studied were the coating thickness, mango bars firmness, syneresis, and colour alterations.
26657585	9	49	theme	product	1419:1425	arg1	value					1400:1404	the commercial value	1385:1404	the commercial value of the final product	1385:1425	This work showed that the coating can improve mango bars sensory characteristics (appearance and firmness) and stability in terms of syneresis, colour and volatiles content during storage increasing the commercial value of the final product.
26657585	2	50	from	concentration	308:320	arg1	solution					390:397	the coating solution	378:397	the coating solution	378:397	The independent variables were the concentration of gellan (L/H90/10) and the concentration of Ca(2+) in the coating solution, as well as the storage time after coating application.
26657585	8	51	theme	Headspace	1087:1095	arg1	Chromatography					1132:1145	Headspace - Solid Phase Micro Extraction-Gas Chromatography	1087:1145	Chromatography	1132:1145	The release of eight volatile compounds from the uncoated and coated mango bars with the selected formulation was analysed by Headspace - Solid Phase Micro Extraction-Gas Chromatography during 9 days of refrigerated storage.
26657585	0	52	theme	ready-to-eat	46:57	arg1	mango					59:63	ready-to-eat mango	46:63	ready-to-eat mango (Mangifera indica L.) bars	46:90	Optimisation of gellan gum edible coating for ready-to-eat mango (Mangifera indica L.) bars.
26657585	0	52	theme	ready-to-eat	46:57	arg1	L.					83:84	Mangifera indica L.	66:84	Mangifera indica L.	66:84	Optimisation of gellan gum edible coating for ready-to-eat mango (Mangifera indica L.) bars.
26657585	1	53	theme	composite	238:246	arg1	CCRD					266:269	CCRD	266:269	CCRD	266:269	The optimisation of an edible coating based on low acyl (L)/high acyl (H) gellan gum for ready-to-eat mango bars was performed through a central composite rotatable design (CCRD).
26657585	1	53	theme	composite	238:246	arg1	design					258:263	a central composite rotatable design	228:263	a central composite rotatable design (CCRD)	228:270	The optimisation of an edible coating based on low acyl (L)/high acyl (H) gellan gum for ready-to-eat mango bars was performed through a central composite rotatable design (CCRD).
26657585	7	54	theme	mM	883:884	arg1	2+					889:890	2+	889:890	2+	889:890	Considering the model predictions, 1.0%wt L/H90/10 with addition of 6 mM Ca(2+) could represent the optimal coating formulation for the mango bars.
26657585	7	54	theme	mM	883:884	arg1	Ca					886:887	6 mM Ca	881:887	6 mM Ca(2+)	881:891	Considering the model predictions, 1.0%wt L/H90/10 with addition of 6 mM Ca(2+) could represent the optimal coating formulation for the mango bars.
26657585	4	55	theme	independent	599:609	arg1	concentration					577:589	Gellan concentration	570:589	Gellan concentration	570:589	Gellan concentration was the independent variable that most influenced the thickness of the coating.
26657585	4	55	theme	independent	599:609	arg1	variable					611:618	the independent variable	595:618	the independent variable that most influenced the thickness of the coating	595:668	Gellan concentration was the independent variable that most influenced the thickness of the coating.
26657585	1	56	theme	rotatable	248:256	arg1	CCRD					266:269	CCRD	266:269	CCRD	266:269	The optimisation of an edible coating based on low acyl (L)/high acyl (H) gellan gum for ready-to-eat mango bars was performed through a central composite rotatable design (CCRD).
26657585	1	56	theme	rotatable	248:256	arg1	design					258:263	a central composite rotatable design	228:263	a central composite rotatable design (CCRD)	228:270	The optimisation of an edible coating based on low acyl (L)/high acyl (H) gellan gum for ready-to-eat mango bars was performed through a central composite rotatable design (CCRD).
26657585	6	57	theme	storage	792:798	arg1	ΔE<5					806:809	ΔE<5	806:809	ΔE<5	806:809	Similarly, the colour alterations were low during the entire storage time (ΔE<5).
26657585	6	57	theme	storage	792:798	arg1	time					800:803	the entire storage time	781:803	the entire storage time (ΔE<5)	781:810	Similarly, the colour alterations were low during the entire storage time (ΔE<5).
26657585	3	58	theme	colour	550:555	arg1	alterations					557:567	colour alterations	550:567	colour alterations	550:567	The response variables studied were the coating thickness, mango bars firmness, syneresis, and colour alterations.
26657585	7	59	theme	coating	921:927	arg1	formulation					929:939	the optimal coating formulation	909:939	the optimal coating formulation for the mango bars	909:958	Considering the model predictions, 1.0%wt L/H90/10 with addition of 6 mM Ca(2+) could represent the optimal coating formulation for the mango bars.
26657585	7	60	theme	wt	852:853	arg1	L/H90/10					855:862	1.0%wt L/H90/10	848:862	1.0%wt L/H90/10 with addition of 6 mM Ca(2+)	848:891	Considering the model predictions, 1.0%wt L/H90/10 with addition of 6 mM Ca(2+) could represent the optimal coating formulation for the mango bars.
26657585	2	61	from	time	423:426	arg1	solution					390:397	the coating solution	378:397	the coating solution	378:397	The independent variables were the concentration of gellan (L/H90/10) and the concentration of Ca(2+) in the coating solution, as well as the storage time after coating application.
26657585	9	62	dep	characteristics	1251:1265	arg1	firmness					1283:1290	firmness	1283:1290	firmness	1283:1290	This work showed that the coating can improve mango bars sensory characteristics (appearance and firmness) and stability in terms of syneresis, colour and volatiles content during storage increasing the commercial value of the final product.
26657585	9	62	dep	characteristics	1251:1265	arg1	appearance					1268:1277	appearance	1268:1277	appearance	1268:1277	This work showed that the coating can improve mango bars sensory characteristics (appearance and firmness) and stability in terms of syneresis, colour and volatiles content during storage increasing the commercial value of the final product.
26657585	9	62	dep	characteristics	1251:1265	arg1	characteristics					1251:1265	mango bars sensory characteristics	1232:1265	mango bars sensory characteristics (appearance and firmness)	1232:1291	This work showed that the coating can improve mango bars sensory characteristics (appearance and firmness) and stability in terms of syneresis, colour and volatiles content during storage increasing the commercial value of the final product.
26657585	8	63	theme	Extraction-Gas	1117:1130	arg1	Chromatography					1132:1145	Headspace - Solid Phase Micro Extraction-Gas Chromatography	1087:1145	Chromatography	1132:1145	The release of eight volatile compounds from the uncoated and coated mango bars with the selected formulation was analysed by Headspace - Solid Phase Micro Extraction-Gas Chromatography during 9 days of refrigerated storage.
26657585	8	64	theme	storage	1177:1183	arg1	days					1156:1159	9 days	1154:1159	9 days of refrigerated storage	1154:1183	The release of eight volatile compounds from the uncoated and coated mango bars with the selected formulation was analysed by Headspace - Solid Phase Micro Extraction-Gas Chromatography during 9 days of refrigerated storage.
26657585	1	65	theme	H	164:164	arg1	gum					174:176	low acyl (L)/high acyl (H) gellan gum	140:176	low acyl (L)/high acyl (H) gellan gum for ready-to-eat mango bars	140:204	The optimisation of an edible coating based on low acyl (L)/high acyl (H) gellan gum for ready-to-eat mango bars was performed through a central composite rotatable design (CCRD).
26657585	7	66	theme	6	881:881	arg1	mM					883:884	mM	883:884	mM	883:884	Considering the model predictions, 1.0%wt L/H90/10 with addition of 6 mM Ca(2+) could represent the optimal coating formulation for the mango bars.
26657585	9	67	theme	bars	1238:1241	arg1	firmness					1283:1290	firmness	1283:1290	firmness	1283:1290	This work showed that the coating can improve mango bars sensory characteristics (appearance and firmness) and stability in terms of syneresis, colour and volatiles content during storage increasing the commercial value of the final product.
26657585	9	67	theme	bars	1238:1241	arg1	appearance					1268:1277	appearance	1268:1277	appearance	1268:1277	This work showed that the coating can improve mango bars sensory characteristics (appearance and firmness) and stability in terms of syneresis, colour and volatiles content during storage increasing the commercial value of the final product.
26657585	9	67	theme	bars	1238:1241	arg1	characteristics					1251:1265	mango bars sensory characteristics	1232:1265	mango bars sensory characteristics (appearance and firmness)	1232:1291	This work showed that the coating can improve mango bars sensory characteristics (appearance and firmness) and stability in terms of syneresis, colour and volatiles content during storage increasing the commercial value of the final product.
26657585	3	68	theme	mango	514:518	arg1	firmness					525:532	mango bars firmness	514:532	mango bars firmness	514:532	The response variables studied were the coating thickness, mango bars firmness, syneresis, and colour alterations.
26657585	7	69	theme	1.0	848:850	arg1	%					851:851	%	851:851	%	851:851	Considering the model predictions, 1.0%wt L/H90/10 with addition of 6 mM Ca(2+) could represent the optimal coating formulation for the mango bars.
26657585	8	70	theme	Micro	1111:1115	arg1	Chromatography					1132:1145	Headspace - Solid Phase Micro Extraction-Gas Chromatography	1087:1145	Chromatography	1132:1145	The release of eight volatile compounds from the uncoated and coated mango bars with the selected formulation was analysed by Headspace - Solid Phase Micro Extraction-Gas Chromatography during 9 days of refrigerated storage.
26657585	8	71	theme	coated	1023:1028	arg1	bars					1036:1039	the uncoated and coated mango bars	1006:1039	the uncoated and coated mango bars	1006:1039	The release of eight volatile compounds from the uncoated and coated mango bars with the selected formulation was analysed by Headspace - Solid Phase Micro Extraction-Gas Chromatography during 9 days of refrigerated storage.
26657585	3	72	theme	coating	495:501	arg1	thickness					503:511	the coating thickness	491:511	the coating thickness	491:511	The response variables studied were the coating thickness, mango bars firmness, syneresis, and colour alterations.
26657585	3	72	theme	coating	495:501	arg1	variables					468:476	The response variables	455:476	The response variables studied	455:484	The response variables studied were the coating thickness, mango bars firmness, syneresis, and colour alterations.
26657585	7	73	theme	%	851:851	arg1	L/H90/10					855:862	1.0%wt L/H90/10	848:862	1.0%wt L/H90/10 with addition of 6 mM Ca(2+)	848:891	Considering the model predictions, 1.0%wt L/H90/10 with addition of 6 mM Ca(2+) could represent the optimal coating formulation for the mango bars.
26657585	1	74	theme	edible	116:121	arg1	coating					123:129	an edible coating	113:129	an edible coating based on low acyl (L)/high acyl (H) gellan gum for ready-to-eat mango bars	113:204	The optimisation of an edible coating based on low acyl (L)/high acyl (H) gellan gum for ready-to-eat mango bars was performed through a central composite rotatable design (CCRD).
26657585	1	75	theme	gellan	167:172	arg1	gum					174:176	low acyl (L)/high acyl (H) gellan gum	140:176	low acyl (L)/high acyl (H) gellan gum for ready-to-eat mango bars	140:204	The optimisation of an edible coating based on low acyl (L)/high acyl (H) gellan gum for ready-to-eat mango bars was performed through a central composite rotatable design (CCRD).
26657585	0	76	theme	mango	59:63	arg1	bars					87:90	ready-to-eat mango (Mangifera indica L.) bars	46:90	ready-to-eat mango (Mangifera indica L.) bars	46:90	Optimisation of gellan gum edible coating for ready-to-eat mango (Mangifera indica L.) bars.
26657585	2	77	from	concentration	351:363	arg1	solution					390:397	the coating solution	378:397	the coating solution	378:397	The independent variables were the concentration of gellan (L/H90/10) and the concentration of Ca(2+) in the coating solution, as well as the storage time after coating application.
26657585	8	78	theme	uncoated	1010:1017	arg1	bars					1036:1039	the uncoated and coated mango bars	1006:1039	the uncoated and coated mango bars	1006:1039	The release of eight volatile compounds from the uncoated and coated mango bars with the selected formulation was analysed by Headspace - Solid Phase Micro Extraction-Gas Chromatography during 9 days of refrigerated storage.
26657585	1	79	theme	coating	123:129	arg1	optimisation					97:108	The optimisation	93:108	The optimisation of an edible coating based on low acyl (L)/high acyl (H) gellan gum for ready-to-eat mango bars	93:204	The optimisation of an edible coating based on low acyl (L)/high acyl (H) gellan gum for ready-to-eat mango bars was performed through a central composite rotatable design (CCRD).
25037360	6	0	theme	subsequent	1278:1287	arg1	gelation					1289:1296	subsequent gelation	1278:1296	subsequent gelation of the precursor droplets	1278:1322	At pH=7.4, the cumulative release of 5-FU was ∼ 50% within 96 h and decreased to ∼ 33% at pH = 5.2, which was attributed to the different solubility of 5-FU as a function of pH. The preparation of composite microgels with controllable dimensions in the range from 42 to 18 μm and with narrow size distribution (polidispersity not exceeding 1.5%) was achieved by the microfluidic emulsification of an aqueous mixture of chitosan and agarose and subsequent gelation of the precursor droplets by cooling.
25037360	1	1	theme	biodegradable	182:194	arg1	materials					196:204	biocompatible and biodegradable materials	164:204	biocompatible and biodegradable materials with cooperative properties	164:232	The preparation of composite biopolymer hydrogels offers the capability to produce biocompatible and biodegradable materials with cooperative properties.
25037360	4	2	theme	composite	583:591	arg1	gels					593:596	composite gels	583:596	composite gels	583:596	In addition, composite gels exhibited a higher stability in acidic aqueous solutions, in comparison with agarose gels.
25037360	5	3	theme	model	769:773	arg1	5-Fluorouracil					792:805	5-Fluorouracil	792:805	5-Fluorouracil	792:805	The drug release properties of the composite hydrogels were tested by loading a model anticancer drug, 5-Fluorouracil, in the hydrogel interior.
25037360	5	3	theme	model	769:773	arg1	drug					786:789	a model anticancer drug	767:789	a model anticancer drug	767:789	The drug release properties of the composite hydrogels were tested by loading a model anticancer drug, 5-Fluorouracil, in the hydrogel interior.
25037360	1	4	with	materials	196:204	arg1	properties					223:232	cooperative properties	211:232	cooperative properties	211:232	The preparation of composite biopolymer hydrogels offers the capability to produce biocompatible and biodegradable materials with cooperative properties.
25037360	3	5	theme	2	535:535	arg1	%					536:536	%	536:536	%	536:536	The elastic modulus of the composite hydrogels increased with agarose concentration reaching the value of 1 kPa for the chitosan/agarose gel with a 2% (w/v) concentration of agarose.
25037360	6	6	dep	distribution	1131:1142	arg1	polidispersity					1145:1158	polidispersity	1145:1158	polidispersity not exceeding 1.5%	1145:1177	At pH=7.4, the cumulative release of 5-FU was ∼ 50% within 96 h and decreased to ∼ 33% at pH = 5.2, which was attributed to the different solubility of 5-FU as a function of pH. The preparation of composite microgels with controllable dimensions in the range from 42 to 18 μm and with narrow size distribution (polidispersity not exceeding 1.5%) was achieved by the microfluidic emulsification of an aqueous mixture of chitosan and agarose and subsequent gelation of the precursor droplets by cooling.
25037360	5	7	theme	composite	724:732	arg1	hydrogels					734:742	the composite hydrogels	720:742	the composite hydrogels	720:742	The drug release properties of the composite hydrogels were tested by loading a model anticancer drug, 5-Fluorouracil, in the hydrogel interior.
25037360	6	8	with	microgels	1041:1049	arg1	distribution					1131:1142	narrow size distribution	1119:1142	narrow size distribution (polidispersity not exceeding 1.5%)	1119:1178	At pH=7.4, the cumulative release of 5-FU was ∼ 50% within 96 h and decreased to ∼ 33% at pH = 5.2, which was attributed to the different solubility of 5-FU as a function of pH. The preparation of composite microgels with controllable dimensions in the range from 42 to 18 μm and with narrow size distribution (polidispersity not exceeding 1.5%) was achieved by the microfluidic emulsification of an aqueous mixture of chitosan and agarose and subsequent gelation of the precursor droplets by cooling.
25037360	6	8	with	microgels	1041:1049	arg1	dimensions					1069:1078	controllable dimensions	1056:1078	controllable dimensions in the range from 42 to 18 μm	1056:1108	At pH=7.4, the cumulative release of 5-FU was ∼ 50% within 96 h and decreased to ∼ 33% at pH = 5.2, which was attributed to the different solubility of 5-FU as a function of pH. The preparation of composite microgels with controllable dimensions in the range from 42 to 18 μm and with narrow size distribution (polidispersity not exceeding 1.5%) was achieved by the microfluidic emulsification of an aqueous mixture of chitosan and agarose and subsequent gelation of the precursor droplets by cooling.
25037360	6	9	theme	size	1126:1129	arg1	distribution					1131:1142	narrow size distribution	1119:1142	narrow size distribution (polidispersity not exceeding 1.5%)	1119:1178	At pH=7.4, the cumulative release of 5-FU was ∼ 50% within 96 h and decreased to ∼ 33% at pH = 5.2, which was attributed to the different solubility of 5-FU as a function of pH. The preparation of composite microgels with controllable dimensions in the range from 42 to 18 μm and with narrow size distribution (polidispersity not exceeding 1.5%) was achieved by the microfluidic emulsification of an aqueous mixture of chitosan and agarose and subsequent gelation of the precursor droplets by cooling.
25037360	3	10	theme	elastic	391:397	arg1	modulus					399:405	The elastic modulus	387:405	The elastic modulus of the composite hydrogels	387:432	The elastic modulus of the composite hydrogels increased with agarose concentration reaching the value of 1 kPa for the chitosan/agarose gel with a 2% (w/v) concentration of agarose.
25037360	3	11	theme	chitosan/agarose	507:522	arg1	gel					524:526	the chitosan/agarose gel	503:526	the chitosan/agarose gel	503:526	The elastic modulus of the composite hydrogels increased with agarose concentration reaching the value of 1 kPa for the chitosan/agarose gel with a 2% (w/v) concentration of agarose.
25037360	6	12	theme	chitosan	1253:1260	arg1	gelation					1289:1296	subsequent gelation	1278:1296	subsequent gelation of the precursor droplets	1278:1322	At pH=7.4, the cumulative release of 5-FU was ∼ 50% within 96 h and decreased to ∼ 33% at pH = 5.2, which was attributed to the different solubility of 5-FU as a function of pH. The preparation of composite microgels with controllable dimensions in the range from 42 to 18 μm and with narrow size distribution (polidispersity not exceeding 1.5%) was achieved by the microfluidic emulsification of an aqueous mixture of chitosan and agarose and subsequent gelation of the precursor droplets by cooling.
25037360	6	12	theme	chitosan	1253:1260	arg1	mixture					1242:1248	an aqueous mixture	1231:1248	an aqueous mixture of chitosan and agarose	1231:1272	At pH=7.4, the cumulative release of 5-FU was ∼ 50% within 96 h and decreased to ∼ 33% at pH = 5.2, which was attributed to the different solubility of 5-FU as a function of pH. The preparation of composite microgels with controllable dimensions in the range from 42 to 18 μm and with narrow size distribution (polidispersity not exceeding 1.5%) was achieved by the microfluidic emulsification of an aqueous mixture of chitosan and agarose and subsequent gelation of the precursor droplets by cooling.
25037360	4	13	theme	acidic	630:635	arg1	solutions					645:653	acidic aqueous solutions	630:653	acidic aqueous solutions	630:653	In addition, composite gels exhibited a higher stability in acidic aqueous solutions, in comparison with agarose gels.
25037360	6	14	theme	5-FU	871:874	arg1	%					884:884	∼ 50%	880:884	∼ 50%	880:884	At pH=7.4, the cumulative release of 5-FU was ∼ 50% within 96 h and decreased to ∼ 33% at pH = 5.2, which was attributed to the different solubility of 5-FU as a function of pH. The preparation of composite microgels with controllable dimensions in the range from 42 to 18 μm and with narrow size distribution (polidispersity not exceeding 1.5%) was achieved by the microfluidic emulsification of an aqueous mixture of chitosan and agarose and subsequent gelation of the precursor droplets by cooling.
25037360	6	14	theme	5-FU	871:874	arg1	release					860:866	the cumulative release	845:866	the cumulative release of 5-FU	845:874	At pH=7.4, the cumulative release of 5-FU was ∼ 50% within 96 h and decreased to ∼ 33% at pH = 5.2, which was attributed to the different solubility of 5-FU as a function of pH. The preparation of composite microgels with controllable dimensions in the range from 42 to 18 μm and with narrow size distribution (polidispersity not exceeding 1.5%) was achieved by the microfluidic emulsification of an aqueous mixture of chitosan and agarose and subsequent gelation of the precursor droplets by cooling.
25037360	3	15	theme	composite	414:422	arg1	hydrogels					424:432	the composite hydrogels	410:432	the composite hydrogels	410:432	The elastic modulus of the composite hydrogels increased with agarose concentration reaching the value of 1 kPa for the chitosan/agarose gel with a 2% (w/v) concentration of agarose.
25037360	1	16	theme	cooperative	211:221	arg1	properties					223:232	cooperative properties	211:232	cooperative properties	211:232	The preparation of composite biopolymer hydrogels offers the capability to produce biocompatible and biodegradable materials with cooperative properties.
25037360	6	17	dep	preparation	1016:1026	arg1	pH.					1008:1010	pH. The preparation of composite microgels with controllable dimensions in the range from 42 to 18 μm and with narrow size distribution (polidispersity not exceeding 1.5%)	1008:1178	pH. The preparation of composite microgels with controllable dimensions in the range from 42 to 18 μm and with narrow size distribution (polidispersity not exceeding 1.5%)	1008:1178	At pH=7.4, the cumulative release of 5-FU was ∼ 50% within 96 h and decreased to ∼ 33% at pH = 5.2, which was attributed to the different solubility of 5-FU as a function of pH. The preparation of composite microgels with controllable dimensions in the range from 42 to 18 μm and with narrow size distribution (polidispersity not exceeding 1.5%) was achieved by the microfluidic emulsification of an aqueous mixture of chitosan and agarose and subsequent gelation of the precursor droplets by cooling.
25037360	1	18	theme	composite	100:108	arg1	hydrogels					121:129	composite biopolymer hydrogels	100:129	composite biopolymer hydrogels	100:129	The preparation of composite biopolymer hydrogels offers the capability to produce biocompatible and biodegradable materials with cooperative properties.
25037360	6	19	theme	microfluidic	1200:1211	arg1	emulsification					1213:1226	the microfluidic emulsification	1196:1226	the microfluidic emulsification of an aqueous mixture of chitosan and agarose and subsequent gelation of the precursor droplets	1196:1322	At pH=7.4, the cumulative release of 5-FU was ∼ 50% within 96 h and decreased to ∼ 33% at pH = 5.2, which was attributed to the different solubility of 5-FU as a function of pH. The preparation of composite microgels with controllable dimensions in the range from 42 to 18 μm and with narrow size distribution (polidispersity not exceeding 1.5%) was achieved by the microfluidic emulsification of an aqueous mixture of chitosan and agarose and subsequent gelation of the precursor droplets by cooling.
25037360	4	20	theme	agarose	675:681	arg1	gels					683:686	agarose gels	675:686	agarose gels	675:686	In addition, composite gels exhibited a higher stability in acidic aqueous solutions, in comparison with agarose gels.
25037360	0	21	theme	Chitosan/agarose	0:15	arg1	hydrogels					17:25	Chitosan/agarose hydrogels	0:25	Chitosan/agarose hydrogels: cooperative properties and microfluidic preparation.	0:79	Chitosan/agarose hydrogels: cooperative properties and microfluidic preparation.
25037360	1	22	theme	biopolymer	110:119	arg1	hydrogels					121:129	composite biopolymer hydrogels	100:129	composite biopolymer hydrogels	100:129	The preparation of composite biopolymer hydrogels offers the capability to produce biocompatible and biodegradable materials with cooperative properties.
25037360	6	23	from	dimensions	1069:1078	arg1	μm					1107:1108	42 to 18 μm	1098:1108	42 to 18 μm	1098:1108	At pH=7.4, the cumulative release of 5-FU was ∼ 50% within 96 h and decreased to ∼ 33% at pH = 5.2, which was attributed to the different solubility of 5-FU as a function of pH. The preparation of composite microgels with controllable dimensions in the range from 42 to 18 μm and with narrow size distribution (polidispersity not exceeding 1.5%) was achieved by the microfluidic emulsification of an aqueous mixture of chitosan and agarose and subsequent gelation of the precursor droplets by cooling.
25037360	6	23	from	dimensions	1069:1078	arg1	range					1087:1091	the range	1083:1091	the range from 42 to 18 μm	1083:1108	At pH=7.4, the cumulative release of 5-FU was ∼ 50% within 96 h and decreased to ∼ 33% at pH = 5.2, which was attributed to the different solubility of 5-FU as a function of pH. The preparation of composite microgels with controllable dimensions in the range from 42 to 18 μm and with narrow size distribution (polidispersity not exceeding 1.5%) was achieved by the microfluidic emulsification of an aqueous mixture of chitosan and agarose and subsequent gelation of the precursor droplets by cooling.
25037360	6	24	theme	5-FU	986:989	arg1	solubility					972:981	the different solubility	958:981	the different solubility of 5-FU	958:989	At pH=7.4, the cumulative release of 5-FU was ∼ 50% within 96 h and decreased to ∼ 33% at pH = 5.2, which was attributed to the different solubility of 5-FU as a function of pH. The preparation of composite microgels with controllable dimensions in the range from 42 to 18 μm and with narrow size distribution (polidispersity not exceeding 1.5%) was achieved by the microfluidic emulsification of an aqueous mixture of chitosan and agarose and subsequent gelation of the precursor droplets by cooling.
25037360	0	25	theme	cooperative	28:38	arg1	properties					40:49	cooperative properties	28:49	cooperative properties	28:49	Chitosan/agarose hydrogels: cooperative properties and microfluidic preparation.
25037360	5	26	theme	release	698:704	arg1	properties					706:715	The drug release properties	689:715	The drug release properties of the composite hydrogels	689:742	The drug release properties of the composite hydrogels were tested by loading a model anticancer drug, 5-Fluorouracil, in the hydrogel interior.
25037360	2	27	theme	composite	326:334	arg1	hydrogels					336:344	composite hydrogels	326:344	composite hydrogels	326:344	In this paper, two natural polymers, namely, chitosan and agarose were employed to prepare composite hydrogels with dual pH and temperature properties.
25037360	3	28	theme	kPa	495:497	arg1	value					484:488	the value	480:488	the value of 1 kPa for the chitosan/agarose gel	480:526	The elastic modulus of the composite hydrogels increased with agarose concentration reaching the value of 1 kPa for the chitosan/agarose gel with a 2% (w/v) concentration of agarose.
25037360	5	29	theme	hydrogel	815:822	arg1	interior					824:831	the hydrogel interior	811:831	the hydrogel interior	811:831	The drug release properties of the composite hydrogels were tested by loading a model anticancer drug, 5-Fluorouracil, in the hydrogel interior.
25037360	6	30	theme	aqueous	1234:1240	arg1	mixture					1242:1248	an aqueous mixture	1231:1248	an aqueous mixture of chitosan and agarose	1231:1272	At pH=7.4, the cumulative release of 5-FU was ∼ 50% within 96 h and decreased to ∼ 33% at pH = 5.2, which was attributed to the different solubility of 5-FU as a function of pH. The preparation of composite microgels with controllable dimensions in the range from 42 to 18 μm and with narrow size distribution (polidispersity not exceeding 1.5%) was achieved by the microfluidic emulsification of an aqueous mixture of chitosan and agarose and subsequent gelation of the precursor droplets by cooling.
25037360	6	31	theme	controllable	1056:1067	arg1	dimensions					1069:1078	controllable dimensions	1056:1078	controllable dimensions in the range from 42 to 18 μm	1056:1108	At pH=7.4, the cumulative release of 5-FU was ∼ 50% within 96 h and decreased to ∼ 33% at pH = 5.2, which was attributed to the different solubility of 5-FU as a function of pH. The preparation of composite microgels with controllable dimensions in the range from 42 to 18 μm and with narrow size distribution (polidispersity not exceeding 1.5%) was achieved by the microfluidic emulsification of an aqueous mixture of chitosan and agarose and subsequent gelation of the precursor droplets by cooling.
25037360	1	32	theme	biocompatible	164:176	arg1	materials					196:204	biocompatible and biodegradable materials	164:204	biocompatible and biodegradable materials with cooperative properties	164:232	The preparation of composite biopolymer hydrogels offers the capability to produce biocompatible and biodegradable materials with cooperative properties.
25037360	5	33	theme	anticancer	775:784	arg1	5-Fluorouracil					792:805	5-Fluorouracil	792:805	5-Fluorouracil	792:805	The drug release properties of the composite hydrogels were tested by loading a model anticancer drug, 5-Fluorouracil, in the hydrogel interior.
25037360	5	33	theme	anticancer	775:784	arg1	drug					786:789	a model anticancer drug	767:789	a model anticancer drug	767:789	The drug release properties of the composite hydrogels were tested by loading a model anticancer drug, 5-Fluorouracil, in the hydrogel interior.
25037360	6	34	theme	∼	915:915	arg1	%					919:919	∼ 33%	915:919	∼ 33%	915:919	At pH=7.4, the cumulative release of 5-FU was ∼ 50% within 96 h and decreased to ∼ 33% at pH = 5.2, which was attributed to the different solubility of 5-FU as a function of pH. The preparation of composite microgels with controllable dimensions in the range from 42 to 18 μm and with narrow size distribution (polidispersity not exceeding 1.5%) was achieved by the microfluidic emulsification of an aqueous mixture of chitosan and agarose and subsequent gelation of the precursor droplets by cooling.
25037360	6	35	theme	cumulative	849:858	arg1	%					884:884	∼ 50%	880:884	∼ 50%	880:884	At pH=7.4, the cumulative release of 5-FU was ∼ 50% within 96 h and decreased to ∼ 33% at pH = 5.2, which was attributed to the different solubility of 5-FU as a function of pH. The preparation of composite microgels with controllable dimensions in the range from 42 to 18 μm and with narrow size distribution (polidispersity not exceeding 1.5%) was achieved by the microfluidic emulsification of an aqueous mixture of chitosan and agarose and subsequent gelation of the precursor droplets by cooling.
25037360	6	35	theme	cumulative	849:858	arg1	release					860:866	the cumulative release	845:866	the cumulative release of 5-FU	845:874	At pH=7.4, the cumulative release of 5-FU was ∼ 50% within 96 h and decreased to ∼ 33% at pH = 5.2, which was attributed to the different solubility of 5-FU as a function of pH. The preparation of composite microgels with controllable dimensions in the range from 42 to 18 μm and with narrow size distribution (polidispersity not exceeding 1.5%) was achieved by the microfluidic emulsification of an aqueous mixture of chitosan and agarose and subsequent gelation of the precursor droplets by cooling.
25037360	1	36	theme	hydrogels	121:129	arg1	preparation					85:95	The preparation	81:95	The preparation of composite biopolymer hydrogels	81:129	The preparation of composite biopolymer hydrogels offers the capability to produce biocompatible and biodegradable materials with cooperative properties.
25037360	6	37	theme	different	962:970	arg1	solubility					972:981	the different solubility	958:981	the different solubility of 5-FU	958:989	At pH=7.4, the cumulative release of 5-FU was ∼ 50% within 96 h and decreased to ∼ 33% at pH = 5.2, which was attributed to the different solubility of 5-FU as a function of pH. The preparation of composite microgels with controllable dimensions in the range from 42 to 18 μm and with narrow size distribution (polidispersity not exceeding 1.5%) was achieved by the microfluidic emulsification of an aqueous mixture of chitosan and agarose and subsequent gelation of the precursor droplets by cooling.
25037360	3	38	theme	agarose	449:455	arg1	concentration					457:469	agarose concentration	449:469	agarose concentration	449:469	The elastic modulus of the composite hydrogels increased with agarose concentration reaching the value of 1 kPa for the chitosan/agarose gel with a 2% (w/v) concentration of agarose.
25037360	6	39	theme	droplets	1315:1322	arg1	gelation					1289:1296	subsequent gelation	1278:1296	subsequent gelation of the precursor droplets	1278:1322	At pH=7.4, the cumulative release of 5-FU was ∼ 50% within 96 h and decreased to ∼ 33% at pH = 5.2, which was attributed to the different solubility of 5-FU as a function of pH. The preparation of composite microgels with controllable dimensions in the range from 42 to 18 μm and with narrow size distribution (polidispersity not exceeding 1.5%) was achieved by the microfluidic emulsification of an aqueous mixture of chitosan and agarose and subsequent gelation of the precursor droplets by cooling.
25037360	6	39	theme	droplets	1315:1322	arg1	mixture					1242:1248	an aqueous mixture	1231:1248	an aqueous mixture of chitosan and agarose	1231:1272	At pH=7.4, the cumulative release of 5-FU was ∼ 50% within 96 h and decreased to ∼ 33% at pH = 5.2, which was attributed to the different solubility of 5-FU as a function of pH. The preparation of composite microgels with controllable dimensions in the range from 42 to 18 μm and with narrow size distribution (polidispersity not exceeding 1.5%) was achieved by the microfluidic emulsification of an aqueous mixture of chitosan and agarose and subsequent gelation of the precursor droplets by cooling.
25037360	4	40	with	comparison	659:668	arg1	gels					683:686	agarose gels	675:686	agarose gels	675:686	In addition, composite gels exhibited a higher stability in acidic aqueous solutions, in comparison with agarose gels.
25037360	3	41	theme	hydrogels	424:432	arg1	modulus					399:405	The elastic modulus	387:405	The elastic modulus of the composite hydrogels	387:432	The elastic modulus of the composite hydrogels increased with agarose concentration reaching the value of 1 kPa for the chitosan/agarose gel with a 2% (w/v) concentration of agarose.
25037360	6	42	theme	agarose	1266:1272	arg1	gelation					1289:1296	subsequent gelation	1278:1296	subsequent gelation of the precursor droplets	1278:1322	At pH=7.4, the cumulative release of 5-FU was ∼ 50% within 96 h and decreased to ∼ 33% at pH = 5.2, which was attributed to the different solubility of 5-FU as a function of pH. The preparation of composite microgels with controllable dimensions in the range from 42 to 18 μm and with narrow size distribution (polidispersity not exceeding 1.5%) was achieved by the microfluidic emulsification of an aqueous mixture of chitosan and agarose and subsequent gelation of the precursor droplets by cooling.
25037360	6	42	theme	agarose	1266:1272	arg1	mixture					1242:1248	an aqueous mixture	1231:1248	an aqueous mixture of chitosan and agarose	1231:1272	At pH=7.4, the cumulative release of 5-FU was ∼ 50% within 96 h and decreased to ∼ 33% at pH = 5.2, which was attributed to the different solubility of 5-FU as a function of pH. The preparation of composite microgels with controllable dimensions in the range from 42 to 18 μm and with narrow size distribution (polidispersity not exceeding 1.5%) was achieved by the microfluidic emulsification of an aqueous mixture of chitosan and agarose and subsequent gelation of the precursor droplets by cooling.
25037360	6	43	theme	gelation	1289:1296	arg1	emulsification					1213:1226	the microfluidic emulsification	1196:1226	the microfluidic emulsification of an aqueous mixture of chitosan and agarose and subsequent gelation of the precursor droplets	1196:1322	At pH=7.4, the cumulative release of 5-FU was ∼ 50% within 96 h and decreased to ∼ 33% at pH = 5.2, which was attributed to the different solubility of 5-FU as a function of pH. The preparation of composite microgels with controllable dimensions in the range from 42 to 18 μm and with narrow size distribution (polidispersity not exceeding 1.5%) was achieved by the microfluidic emulsification of an aqueous mixture of chitosan and agarose and subsequent gelation of the precursor droplets by cooling.
25037360	2	44	theme	natural	254:260	arg1	polymers					262:269	two natural polymers	250:269	two natural polymers	250:269	In this paper, two natural polymers, namely, chitosan and agarose were employed to prepare composite hydrogels with dual pH and temperature properties.
25037360	2	44	theme	natural	254:260	arg1	agarose					293:299	agarose	293:299	agarose	293:299	In this paper, two natural polymers, namely, chitosan and agarose were employed to prepare composite hydrogels with dual pH and temperature properties.
25037360	2	44	theme	natural	254:260	arg1	chitosan					280:287	chitosan	280:287	chitosan	280:287	In this paper, two natural polymers, namely, chitosan and agarose were employed to prepare composite hydrogels with dual pH and temperature properties.
25037360	6	45	theme	preparation	1016:1026	arg1	function					996:1003	a function	994:1003	a function of pH. The preparation of composite microgels with controllable dimensions in the range from 42 to 18 μm and with narrow size distribution (polidispersity not exceeding 1.5%)	994:1178	At pH=7.4, the cumulative release of 5-FU was ∼ 50% within 96 h and decreased to ∼ 33% at pH = 5.2, which was attributed to the different solubility of 5-FU as a function of pH. The preparation of composite microgels with controllable dimensions in the range from 42 to 18 μm and with narrow size distribution (polidispersity not exceeding 1.5%) was achieved by the microfluidic emulsification of an aqueous mixture of chitosan and agarose and subsequent gelation of the precursor droplets by cooling.
25037360	2	46	theme	temperature	363:373	arg1	properties					375:384	temperature properties	363:384	temperature properties	363:384	In this paper, two natural polymers, namely, chitosan and agarose were employed to prepare composite hydrogels with dual pH and temperature properties.
25037360	0	47	theme	microfluidic	55:66	arg1	preparation					68:78	microfluidic preparation	55:78	microfluidic preparation	55:78	Chitosan/agarose hydrogels: cooperative properties and microfluidic preparation.
25037360	6	48	theme	precursor	1305:1313	arg1	droplets					1315:1322	the precursor droplets	1301:1322	the precursor droplets	1301:1322	At pH=7.4, the cumulative release of 5-FU was ∼ 50% within 96 h and decreased to ∼ 33% at pH = 5.2, which was attributed to the different solubility of 5-FU as a function of pH. The preparation of composite microgels with controllable dimensions in the range from 42 to 18 μm and with narrow size distribution (polidispersity not exceeding 1.5%) was achieved by the microfluidic emulsification of an aqueous mixture of chitosan and agarose and subsequent gelation of the precursor droplets by cooling.
25037360	6	49	theme	mixture	1242:1248	arg1	emulsification					1213:1226	the microfluidic emulsification	1196:1226	the microfluidic emulsification of an aqueous mixture of chitosan and agarose and subsequent gelation of the precursor droplets	1196:1322	At pH=7.4, the cumulative release of 5-FU was ∼ 50% within 96 h and decreased to ∼ 33% at pH = 5.2, which was attributed to the different solubility of 5-FU as a function of pH. The preparation of composite microgels with controllable dimensions in the range from 42 to 18 μm and with narrow size distribution (polidispersity not exceeding 1.5%) was achieved by the microfluidic emulsification of an aqueous mixture of chitosan and agarose and subsequent gelation of the precursor droplets by cooling.
25037360	6	50	theme	narrow	1119:1124	arg1	distribution					1131:1142	narrow size distribution	1119:1142	narrow size distribution (polidispersity not exceeding 1.5%)	1119:1178	At pH=7.4, the cumulative release of 5-FU was ∼ 50% within 96 h and decreased to ∼ 33% at pH = 5.2, which was attributed to the different solubility of 5-FU as a function of pH. The preparation of composite microgels with controllable dimensions in the range from 42 to 18 μm and with narrow size distribution (polidispersity not exceeding 1.5%) was achieved by the microfluidic emulsification of an aqueous mixture of chitosan and agarose and subsequent gelation of the precursor droplets by cooling.
25037360	6	51	theme	microgels	1041:1049	arg1	preparation					1016:1026	The preparation	1012:1026	pH. The preparation of composite microgels with controllable dimensions in the range from 42 to 18 μm and with narrow size distribution (polidispersity not exceeding 1.5%)	1008:1178	At pH=7.4, the cumulative release of 5-FU was ∼ 50% within 96 h and decreased to ∼ 33% at pH = 5.2, which was attributed to the different solubility of 5-FU as a function of pH. The preparation of composite microgels with controllable dimensions in the range from 42 to 18 μm and with narrow size distribution (polidispersity not exceeding 1.5%) was achieved by the microfluidic emulsification of an aqueous mixture of chitosan and agarose and subsequent gelation of the precursor droplets by cooling.
25037360	6	52	from	pH=7.4	837:842	arg1	%					884:884	∼ 50%	880:884	∼ 50%	880:884	At pH=7.4, the cumulative release of 5-FU was ∼ 50% within 96 h and decreased to ∼ 33% at pH = 5.2, which was attributed to the different solubility of 5-FU as a function of pH. The preparation of composite microgels with controllable dimensions in the range from 42 to 18 μm and with narrow size distribution (polidispersity not exceeding 1.5%) was achieved by the microfluidic emulsification of an aqueous mixture of chitosan and agarose and subsequent gelation of the precursor droplets by cooling.
25037360	6	52	from	pH=7.4	837:842	arg1	release					860:866	the cumulative release	845:866	the cumulative release of 5-FU	845:874	At pH=7.4, the cumulative release of 5-FU was ∼ 50% within 96 h and decreased to ∼ 33% at pH = 5.2, which was attributed to the different solubility of 5-FU as a function of pH. The preparation of composite microgels with controllable dimensions in the range from 42 to 18 μm and with narrow size distribution (polidispersity not exceeding 1.5%) was achieved by the microfluidic emulsification of an aqueous mixture of chitosan and agarose and subsequent gelation of the precursor droplets by cooling.
25037360	6	53	theme	composite	1031:1039	arg1	microgels					1041:1049	composite microgels	1031:1049	composite microgels with controllable dimensions in the range from 42 to 18 μm and with narrow size distribution (polidispersity not exceeding 1.5%)	1031:1178	At pH=7.4, the cumulative release of 5-FU was ∼ 50% within 96 h and decreased to ∼ 33% at pH = 5.2, which was attributed to the different solubility of 5-FU as a function of pH. The preparation of composite microgels with controllable dimensions in the range from 42 to 18 μm and with narrow size distribution (polidispersity not exceeding 1.5%) was achieved by the microfluidic emulsification of an aqueous mixture of chitosan and agarose and subsequent gelation of the precursor droplets by cooling.
25037360	3	54	theme	agarose	561:567	arg1	concentration					544:556	a 2% (w/v) concentration	533:556	a 2% (w/v) concentration of agarose	533:567	The elastic modulus of the composite hydrogels increased with agarose concentration reaching the value of 1 kPa for the chitosan/agarose gel with a 2% (w/v) concentration of agarose.
25037360	4	55	theme	higher	610:615	arg1	stability					617:625	a higher stability	608:625	a higher stability in acidic aqueous solutions	608:653	In addition, composite gels exhibited a higher stability in acidic aqueous solutions, in comparison with agarose gels.
25037360	5	56	theme	drug	693:696	arg1	properties					706:715	The drug release properties	689:715	The drug release properties of the composite hydrogels	689:742	The drug release properties of the composite hydrogels were tested by loading a model anticancer drug, 5-Fluorouracil, in the hydrogel interior.
25037360	2	57	theme	dual	351:354	arg1	pH					356:357	dual pH	351:357	dual pH	351:357	In this paper, two natural polymers, namely, chitosan and agarose were employed to prepare composite hydrogels with dual pH and temperature properties.
25037360	5	58	from	loading	759:765	arg1	interior					824:831	the hydrogel interior	811:831	the hydrogel interior	811:831	The drug release properties of the composite hydrogels were tested by loading a model anticancer drug, 5-Fluorouracil, in the hydrogel interior.
25037360	0	59	dep	hydrogels	17:25	arg1	properties					40:49	cooperative properties	28:49	cooperative properties	28:49	Chitosan/agarose hydrogels: cooperative properties and microfluidic preparation.
25037360	0	59	dep	hydrogels	17:25	arg1	preparation					68:78	microfluidic preparation	55:78	microfluidic preparation	55:78	Chitosan/agarose hydrogels: cooperative properties and microfluidic preparation.
25037360	4	60	theme	aqueous	637:643	arg1	solutions					645:653	acidic aqueous solutions	630:653	acidic aqueous solutions	630:653	In addition, composite gels exhibited a higher stability in acidic aqueous solutions, in comparison with agarose gels.
25037360	3	61	theme	%	536:536	arg1	concentration					544:556	a 2% (w/v) concentration	533:556	a 2% (w/v) concentration of agarose	533:567	The elastic modulus of the composite hydrogels increased with agarose concentration reaching the value of 1 kPa for the chitosan/agarose gel with a 2% (w/v) concentration of agarose.
25037360	6	62	dep	18	1104:1105	arg1	to					1101:1102	to	1101:1102	to	1101:1102	At pH=7.4, the cumulative release of 5-FU was ∼ 50% within 96 h and decreased to ∼ 33% at pH = 5.2, which was attributed to the different solubility of 5-FU as a function of pH. The preparation of composite microgels with controllable dimensions in the range from 42 to 18 μm and with narrow size distribution (polidispersity not exceeding 1.5%) was achieved by the microfluidic emulsification of an aqueous mixture of chitosan and agarose and subsequent gelation of the precursor droplets by cooling.
25037360	4	63	from	stability	617:625	arg1	solutions					645:653	acidic aqueous solutions	630:653	acidic aqueous solutions	630:653	In addition, composite gels exhibited a higher stability in acidic aqueous solutions, in comparison with agarose gels.
25037360	3	64	dep	%	536:536	arg1	w/v					539:541	w/v	539:541	w/v	539:541	The elastic modulus of the composite hydrogels increased with agarose concentration reaching the value of 1 kPa for the chitosan/agarose gel with a 2% (w/v) concentration of agarose.
25037360	6	65	from	μm	1107:1108	arg1	dimensions					1069:1078	controllable dimensions	1056:1078	controllable dimensions in the range from 42 to 18 μm	1056:1108	At pH=7.4, the cumulative release of 5-FU was ∼ 50% within 96 h and decreased to ∼ 33% at pH = 5.2, which was attributed to the different solubility of 5-FU as a function of pH. The preparation of composite microgels with controllable dimensions in the range from 42 to 18 μm and with narrow size distribution (polidispersity not exceeding 1.5%) was achieved by the microfluidic emulsification of an aqueous mixture of chitosan and agarose and subsequent gelation of the precursor droplets by cooling.
25037360	6	65	from	μm	1107:1108	arg1	range					1087:1091	the range	1083:1091	the range from 42 to 18 μm	1083:1108	At pH=7.4, the cumulative release of 5-FU was ∼ 50% within 96 h and decreased to ∼ 33% at pH = 5.2, which was attributed to the different solubility of 5-FU as a function of pH. The preparation of composite microgels with controllable dimensions in the range from 42 to 18 μm and with narrow size distribution (polidispersity not exceeding 1.5%) was achieved by the microfluidic emulsification of an aqueous mixture of chitosan and agarose and subsequent gelation of the precursor droplets by cooling.
25037360	5	66	theme	hydrogels	734:742	arg1	properties					706:715	The drug release properties	689:715	The drug release properties of the composite hydrogels	689:742	The drug release properties of the composite hydrogels were tested by loading a model anticancer drug, 5-Fluorouracil, in the hydrogel interior.
26794749	5	0	theme	4wt	800:802	arg1	graphene					805:812	4wt% graphene	800:812	4wt% graphene added	800:818	Water contact angles result showed that the addition of graphene decreased hydrophilic property since water contact angle of BC increased from 51.2° to 84.3° with 4wt% graphene added.
26794749	1	1	theme	building	181:188	arg1	nanocomposites					200:213	building polymeric nanocomposites	181:213	building polymeric nanocomposites	181:213	Graphene has been considered to be a promising nanofiller material for building polymeric nanocomposites because it has large specific surface area and unique mechanical property.
26794749	5	2	theme	BC	762:763	arg1	angle					753:757	water contact angle	739:757	water contact angle of BC	739:763	Water contact angles result showed that the addition of graphene decreased hydrophilic property since water contact angle of BC increased from 51.2° to 84.3° with 4wt% graphene added.
26794749	6	3	theme	mechanical	825:834	arg1	performances					836:847	The mechanical performances	821:847	The mechanical performances of BC/graphene composites	821:873	The mechanical performances of BC/graphene composites were highly evaluated.
26794749	7	4	theme	pristine	915:922	arg1	BC					924:925	pristine BC	915:925	pristine BC	915:925	When compared to pristine BC, the incorporation of 4wt% graphene improved the tensile strength from 96MPa to 155MPa and Young's modulus from 369MPa to 530MPa, respectively.
26794749	4	5	theme	BC	626:627	arg1	matrix					629:634	the BC matrix	622:634	the BC matrix	622:634	These results indicate graphene nanosheets were successfully impregnated and uniformly dispersed in the BC matrix.
26794749	5	6	theme	graphene	693:700	arg1	addition					681:688	the addition	677:688	the addition of graphene	677:700	Water contact angles result showed that the addition of graphene decreased hydrophilic property since water contact angle of BC increased from 51.2° to 84.3° with 4wt% graphene added.
26794749	5	7	theme	Water	637:641	arg1	angles					651:656	Water contact angles	637:656	Water contact angles result	637:663	Water contact angles result showed that the addition of graphene decreased hydrophilic property since water contact angle of BC increased from 51.2° to 84.3° with 4wt% graphene added.
26794749	2	8	theme	BC/graphene	304:314	arg1	composites					316:325	BC/graphene composites	304:325	BC/graphene composites	304:325	In the study, BC/graphene composites were prepared by a simple blending method.
26794749	1	9	theme	promising	147:155	arg1	material					168:175	a promising nanofiller material	145:175	a promising nanofiller material for building polymeric nanocomposites	145:213	Graphene has been considered to be a promising nanofiller material for building polymeric nanocomposites because it has large specific surface area and unique mechanical property.
26794749	1	9	theme	promising	147:155	arg1	Graphene					110:117	Graphene	110:117	Graphene	110:117	Graphene has been considered to be a promising nanofiller material for building polymeric nanocomposites because it has large specific surface area and unique mechanical property.
26794749	5	10	theme	angles	651:656	arg1	result					658:663	Water contact angles result	637:663	Water contact angles result	637:663	Water contact angles result showed that the addition of graphene decreased hydrophilic property since water contact angle of BC increased from 51.2° to 84.3° with 4wt% graphene added.
26794749	5	11	theme	contact	643:649	arg1	angles					651:656	Water contact angles	637:656	Water contact angles result	637:663	Water contact angles result showed that the addition of graphene decreased hydrophilic property since water contact angle of BC increased from 51.2° to 84.3° with 4wt% graphene added.
26794749	1	12	theme	polymeric	190:198	arg1	nanocomposites					200:213	building polymeric nanocomposites	181:213	building polymeric nanocomposites	181:213	Graphene has been considered to be a promising nanofiller material for building polymeric nanocomposites because it has large specific surface area and unique mechanical property.
26794749	3	13	theme	resulting	374:382	arg1	structure					384:392	structure	384:392	structure	384:392	The resulting structure and thermal stability of the composites were investigated by several techniques including TEM, SEM, XRD, TG and Raman spectrum.
26794749	5	14	theme	%	803:803	arg1	graphene					805:812	4wt% graphene	800:812	4wt% graphene added	800:818	Water contact angles result showed that the addition of graphene decreased hydrophilic property since water contact angle of BC increased from 51.2° to 84.3° with 4wt% graphene added.
26794749	7	15	theme	Young	1018:1022	arg1	modulus					1026:1032	Young's modulus	1018:1032	Young's modulus	1018:1032	When compared to pristine BC, the incorporation of 4wt% graphene improved the tensile strength from 96MPa to 155MPa and Young's modulus from 369MPa to 530MPa, respectively.
26794749	7	16	theme	tensile	976:982	arg1	strength					984:991	the tensile strength	972:991	the tensile strength from 96MPa to 155MPa and Young's modulus	972:1032	When compared to pristine BC, the incorporation of 4wt% graphene improved the tensile strength from 96MPa to 155MPa and Young's modulus from 369MPa to 530MPa, respectively.
26794749	3	17	theme	several	455:461	arg1	techniques					463:472	several techniques	455:472	several techniques including TEM, SEM, XRD, TG and Raman spectrum	455:519	The resulting structure and thermal stability of the composites were investigated by several techniques including TEM, SEM, XRD, TG and Raman spectrum.
26794749	3	17	theme	several	455:461	arg1	TEM					484:486	TEM	484:486	TEM	484:486	The resulting structure and thermal stability of the composites were investigated by several techniques including TEM, SEM, XRD, TG and Raman spectrum.
26794749	3	17	theme	several	455:461	arg1	TG					499:500	TG	499:500	TG	499:500	The resulting structure and thermal stability of the composites were investigated by several techniques including TEM, SEM, XRD, TG and Raman spectrum.
26794749	3	17	theme	several	455:461	arg1	SEM					489:491	SEM	489:491	SEM	489:491	The resulting structure and thermal stability of the composites were investigated by several techniques including TEM, SEM, XRD, TG and Raman spectrum.
26794749	3	17	theme	several	455:461	arg1	spectrum					512:519	Raman spectrum	506:519	Raman spectrum	506:519	The resulting structure and thermal stability of the composites were investigated by several techniques including TEM, SEM, XRD, TG and Raman spectrum.
26794749	3	17	theme	several	455:461	arg1	XRD					494:496	XRD	494:496	XRD	494:496	The resulting structure and thermal stability of the composites were investigated by several techniques including TEM, SEM, XRD, TG and Raman spectrum.
26794749	6	18	theme	BC/graphene	852:862	arg1	composites					864:873	BC/graphene composites	852:873	BC/graphene composites	852:873	The mechanical performances of BC/graphene composites were highly evaluated.
26794749	7	19	theme	%	952:952	arg1	graphene					954:961	4wt% graphene	949:961	4wt% graphene	949:961	When compared to pristine BC, the incorporation of 4wt% graphene improved the tensile strength from 96MPa to 155MPa and Young's modulus from 369MPa to 530MPa, respectively.
26794749	0	20	theme	cellulose/graphene	25:42	arg1	Preparation					0:10	Preparation	0:10	Preparation of bacterial cellulose/graphene	0:42	Preparation of bacterial cellulose/graphene nanosheets composite films with enhanced mechanical performances.
26794749	5	21	theme	water	739:743	arg1	angle					753:757	water contact angle	739:757	water contact angle of BC	739:763	Water contact angles result showed that the addition of graphene decreased hydrophilic property since water contact angle of BC increased from 51.2° to 84.3° with 4wt% graphene added.
26794749	7	22	theme	graphene	954:961	arg1	incorporation					932:944	the incorporation	928:944	the incorporation of 4wt% graphene	928:961	When compared to pristine BC, the incorporation of 4wt% graphene improved the tensile strength from 96MPa to 155MPa and Young's modulus from 369MPa to 530MPa, respectively.
26794749	0	23	theme	bacterial	15:23	arg1	cellulose/graphene					25:42	bacterial cellulose/graphene	15:42	bacterial cellulose/graphene	15:42	Preparation of bacterial cellulose/graphene nanosheets composite films with enhanced mechanical performances.
26794749	5	24	theme	contact	745:751	arg1	angle					753:757	water contact angle	739:757	water contact angle of BC	739:763	Water contact angles result showed that the addition of graphene decreased hydrophilic property since water contact angle of BC increased from 51.2° to 84.3° with 4wt% graphene added.
26794749	7	25	from	96MPa	998:1002	arg1	strength					984:991	the tensile strength	972:991	the tensile strength from 96MPa to 155MPa and Young's modulus	972:1032	When compared to pristine BC, the incorporation of 4wt% graphene improved the tensile strength from 96MPa to 155MPa and Young's modulus from 369MPa to 530MPa, respectively.
26794749	1	26	theme	large	230:234	arg1	area					253:256	large specific surface area	230:256	large specific surface area	230:256	Graphene has been considered to be a promising nanofiller material for building polymeric nanocomposites because it has large specific surface area and unique mechanical property.
26794749	0	27	theme	composite	55:63	arg1	films					65:69	composite films	55:69	composite films with enhanced mechanical performances	55:107	Preparation of bacterial cellulose/graphene nanosheets composite films with enhanced mechanical performances.
26794749	1	28	theme	specific	236:243	arg1	area					253:256	large specific surface area	230:256	large specific surface area	230:256	Graphene has been considered to be a promising nanofiller material for building polymeric nanocomposites because it has large specific surface area and unique mechanical property.
26794749	4	29	theme	graphene	545:552	arg1	nanosheets					554:563	graphene nanosheets	545:563	graphene nanosheets	545:563	These results indicate graphene nanosheets were successfully impregnated and uniformly dispersed in the BC matrix.
26794749	5	30	dep	84.3°	789:793	arg1	to					786:787	to	786:787	to	786:787	Water contact angles result showed that the addition of graphene decreased hydrophilic property since water contact angle of BC increased from 51.2° to 84.3° with 4wt% graphene added.
26794749	3	31	theme	thermal	398:404	arg1	stability					406:414	thermal stability	398:414	thermal stability	398:414	The resulting structure and thermal stability of the composites were investigated by several techniques including TEM, SEM, XRD, TG and Raman spectrum.
26794749	1	32	theme	surface	245:251	arg1	area					253:256	large specific surface area	230:256	large specific surface area	230:256	Graphene has been considered to be a promising nanofiller material for building polymeric nanocomposites because it has large specific surface area and unique mechanical property.
26794749	3	33	theme	composites	423:432	arg1	structure					384:392	structure	384:392	structure	384:392	The resulting structure and thermal stability of the composites were investigated by several techniques including TEM, SEM, XRD, TG and Raman spectrum.
26794749	3	33	theme	composites	423:432	arg1	stability					406:414	thermal stability	398:414	thermal stability	398:414	The resulting structure and thermal stability of the composites were investigated by several techniques including TEM, SEM, XRD, TG and Raman spectrum.
26794749	5	34	theme	hydrophilic	712:722	arg1	property					724:731	hydrophilic property	712:731	hydrophilic property	712:731	Water contact angles result showed that the addition of graphene decreased hydrophilic property since water contact angle of BC increased from 51.2° to 84.3° with 4wt% graphene added.
26794749	3	35	theme	Raman	506:510	arg1	spectrum					512:519	Raman spectrum	506:519	Raman spectrum	506:519	The resulting structure and thermal stability of the composites were investigated by several techniques including TEM, SEM, XRD, TG and Raman spectrum.
26794749	2	36	theme	blending	353:360	arg1	method					362:367	a simple blending method	344:367	a simple blending method	344:367	In the study, BC/graphene composites were prepared by a simple blending method.
26794749	7	37	theme	4wt	949:951	arg1	graphene					954:961	4wt% graphene	949:961	4wt% graphene	949:961	When compared to pristine BC, the incorporation of 4wt% graphene improved the tensile strength from 96MPa to 155MPa and Young's modulus from 369MPa to 530MPa, respectively.
26794749	6	38	theme	composites	864:873	arg1	performances					836:847	The mechanical performances	821:847	The mechanical performances of BC/graphene composites	821:873	The mechanical performances of BC/graphene composites were highly evaluated.
26794749	2	39	theme	simple	346:351	arg1	method					362:367	a simple blending method	344:367	a simple blending method	344:367	In the study, BC/graphene composites were prepared by a simple blending method.
26794749	0	40	theme	mechanical	85:94	arg1	performances					96:107	enhanced mechanical performances	76:107	enhanced mechanical performances	76:107	Preparation of bacterial cellulose/graphene nanosheets composite films with enhanced mechanical performances.
26794749	1	41	contain	has	226:228	arg2	area					253:256	large specific surface area	230:256	large specific surface area	230:256	Graphene has been considered to be a promising nanofiller material for building polymeric nanocomposites because it has large specific surface area and unique mechanical property.
26794749	1	41	contain	has	226:228	arg1	it					223:224	it	223:224	it	223:224	Graphene has been considered to be a promising nanofiller material for building polymeric nanocomposites because it has large specific surface area and unique mechanical property.
26794749	1	41	contain	has	226:228	arg2	property					280:287	unique mechanical property	262:287	unique mechanical property	262:287	Graphene has been considered to be a promising nanofiller material for building polymeric nanocomposites because it has large specific surface area and unique mechanical property.
26794749	0	42	with	films	65:69	arg1	performances					96:107	enhanced mechanical performances	76:107	enhanced mechanical performances	76:107	Preparation of bacterial cellulose/graphene nanosheets composite films with enhanced mechanical performances.
26794749	1	43	theme	nanofiller	157:166	arg1	material					168:175	a promising nanofiller material	145:175	a promising nanofiller material for building polymeric nanocomposites	145:213	Graphene has been considered to be a promising nanofiller material for building polymeric nanocomposites because it has large specific surface area and unique mechanical property.
26794749	1	43	theme	nanofiller	157:166	arg1	Graphene					110:117	Graphene	110:117	Graphene	110:117	Graphene has been considered to be a promising nanofiller material for building polymeric nanocomposites because it has large specific surface area and unique mechanical property.
26794749	1	44	theme	unique	262:267	arg1	property					280:287	unique mechanical property	262:287	unique mechanical property	262:287	Graphene has been considered to be a promising nanofiller material for building polymeric nanocomposites because it has large specific surface area and unique mechanical property.
26794749	0	45	theme	enhanced	76:83	arg1	performances					96:107	enhanced mechanical performances	76:107	enhanced mechanical performances	76:107	Preparation of bacterial cellulose/graphene nanosheets composite films with enhanced mechanical performances.
26794749	1	46	theme	mechanical	269:278	arg1	property					280:287	unique mechanical property	262:287	unique mechanical property	262:287	Graphene has been considered to be a promising nanofiller material for building polymeric nanocomposites because it has large specific surface area and unique mechanical property.
24616374	4	0	theme	mass	778:781	arg1	fraction					783:790	the mass fraction	774:790	the the mass fraction of the hydrogel not consisting of water	770:830	Mineral formation was confirmed by IR and Raman, SEM, ICP-OES, XRD, TEM, SAED, TGA and increases in the the mass fraction of the hydrogel not consisting of water.
24616374	10	1	theme	order	1388:1392	arg1	A > B > C > D					1394:1406	the order A > B > C > D	1384:1406	the order A > B > C > D	1384:1406	Young's moduli and storage moduli decreased in dependence of mineralization medium in the order A > B > C > D, but were significantly higher for samples mineralized in medium E.
24616374	9	2	theme	crystalline	1267:1277	arg1	combination					1252:1262	a combination	1250:1262	a combination of crystalline and amorphous MgP	1250:1295	Mineral formed in medium E was a combination of crystalline and amorphous MgP.
24616374	9	2	theme	crystalline	1267:1277	arg1	Mineral					1219:1225	Mineral	1219:1225	Mineral	1219:1225	Mineral formed in medium E was a combination of crystalline and amorphous MgP.
24616374	9	3	theme	amorphous	1283:1291	arg1	combination					1252:1262	a combination	1250:1262	a combination of crystalline and amorphous MgP	1250:1295	Mineral formed in medium E was a combination of crystalline and amorphous MgP.
24616374	9	3	theme	amorphous	1283:1291	arg1	Mineral					1219:1225	Mineral	1219:1225	Mineral	1219:1225	Mineral formed in medium E was a combination of crystalline and amorphous MgP.
24616374	10	4	from	E	1473:1473	arg1	mineralized					1451:1461	mineralized	1451:1461	mineralized	1451:1461	Young's moduli and storage moduli decreased in dependence of mineralization medium in the order A > B > C > D, but were significantly higher for samples mineralized in medium E.
24616374	11	5	from	A	1635:1635	arg1	mineralized					1613:1623	mineralized	1613:1623	mineralized	1613:1623	The attachment and vitality of osteoblastic MC3T3-E1 cells were higher on samples mineralized in media B-E (containing Mg) than in those mineralized in medium A (not containing Mg).
24616374	6	6	theme	calcium-deficient	1050:1066	arg1	CDHA					1084:1087	CDHA	1084:1087	CDHA	1084:1087	Mg content and amorphicity of mineral formed increased in the order A < B < C < D. Mineral formed in media A and B was calcium-deficient hydroxyapatite (CDHA).
24616374	6	6	theme	calcium-deficient	1050:1066	arg1	hydroxyapatite					1068:1081	calcium-deficient hydroxyapatite	1050:1081	calcium-deficient hydroxyapatite (CDHA)	1050:1088	Mg content and amorphicity of mineral formed increased in the order A < B < C < D. Mineral formed in media A and B was calcium-deficient hydroxyapatite (CDHA).
24616374	5	7	dep	media	920:924	arg1	A-D					926:928	A-D	926:928	media A-D	920:928	Ca was incorporated into mineral to a greater extent than Mg in samples mineralized in media A-D.
24616374	11	8	theme	MC3T3-E1	1520:1527	arg1	cells					1529:1533	osteoblastic MC3T3-E1 cells	1507:1533	osteoblastic MC3T3-E1 cells	1507:1533	The attachment and vitality of osteoblastic MC3T3-E1 cells were higher on samples mineralized in media B-E (containing Mg) than in those mineralized in medium A (not containing Mg).
24616374	3	9	dep	values	598:603	arg1	mol/dm3					605:611	mol/dm3	605:611	mol/dm3	605:611	Mineralization media with CaGP:MgGP concentrations 0.1:0, 0.075:0.025, 0.05:0.05, 0.025:0.075 and 0:0.1 (all values mol/dm3 , denoted A, B, C, D and E, respectively) were compared.
24616374	2	10	theme	ALP-loaded	330:339	arg1	GG					353:354	GG	353:354	GG	353:354	ALP-loaded gellan gum (GG) hydrogels were mineralized by incubation in mineralization media containing calcium and/or magnesium glycerophosphate (CaGP, MgGP).
24616374	2	10	theme	ALP-loaded	330:339	arg1	gum					348:350	ALP-loaded gellan gum	330:350	ALP-loaded gellan gum (GG) hydrogels	330:365	ALP-loaded gellan gum (GG) hydrogels were mineralized by incubation in mineralization media containing calcium and/or magnesium glycerophosphate (CaGP, MgGP).
24616374	5	11	from	media	920:924	arg1	mineralized					905:915	mineralized	905:915	mineralized	905:915	Ca was incorporated into mineral to a greater extent than Mg in samples mineralized in media A-D.
24616374	1	12	theme	desirable	206:214	arg1	hydrogels					195:203	hydrogels	195:203	hydrogels	195:203	Mineralization of hydrogels, desirable for bone regeneration applications, may be achieved enzymatically by incorporation of alkaline phosphatase (ALP).
24616374	12	13	theme	cell	1816:1819	arg1	formation					1821:1829	osteoclast-like cell formation	1800:1829	osteoclast-like cell formation	1800:1829	All samples underwent degradation and supported the adhesion of RAW 264.7 monocytic cells, and samples mineralized in media A and B supported osteoclast-like cell formation.
24616374	0	14	theme	magnesium	130:138	arg1	phases					150:155	calcium and magnesium phosphate phases	118:155	calcium and magnesium phosphate phases	118:155	Generation of composites for bone tissue-engineering applications consisting of gellan gum hydrogels mineralized with calcium and magnesium phosphate phases by enzymatic means.
24616374	5	15	from	mineralized	905:915	arg1	media					920:924	media A-D	920:928	media A-D	920:928	Ca was incorporated into mineral to a greater extent than Mg in samples mineralized in media A-D.
24616374	1	16	theme	bone	220:223	arg1	applications					238:249	bone regeneration applications	220:249	bone regeneration applications	220:249	Mineralization of hydrogels, desirable for bone regeneration applications, may be achieved enzymatically by incorporation of alkaline phosphatase (ALP).
24616374	11	17	dep	attachment	1480:1489	arg1	The					1476:1478	The	1476:1478	The	1476:1478	The attachment and vitality of osteoblastic MC3T3-E1 cells were higher on samples mineralized in media B-E (containing Mg) than in those mineralized in medium A (not containing Mg).
24616374	4	18	from	TGA	749:751	arg1	fraction					783:790	the mass fraction	774:790	the the mass fraction of the hydrogel not consisting of water	770:830	Mineral formation was confirmed by IR and Raman, SEM, ICP-OES, XRD, TEM, SAED, TGA and increases in the the mass fraction of the hydrogel not consisting of water.
24616374	4	19	theme	hydrogel	799:806	arg1	fraction					783:790	the mass fraction	774:790	the the mass fraction of the hydrogel not consisting of water	770:830	Mineral formation was confirmed by IR and Raman, SEM, ICP-OES, XRD, TEM, SAED, TGA and increases in the the mass fraction of the hydrogel not consisting of water.
24616374	0	20	theme	enzymatic	160:168	arg1	means					170:174	enzymatic means	160:174	enzymatic means	160:174	Generation of composites for bone tissue-engineering applications consisting of gellan gum hydrogels mineralized with calcium and magnesium phosphate phases by enzymatic means.
24616374	2	21	contain	containing	422:431	arg2	magnesium					448:456	magnesium glycerophosphate	448:473	magnesium glycerophosphate	448:473	ALP-loaded gellan gum (GG) hydrogels were mineralized by incubation in mineralization media containing calcium and/or magnesium glycerophosphate (CaGP, MgGP).
24616374	2	21	contain	containing	422:431	arg2	calcium					433:439	calcium	433:439	calcium	433:439	ALP-loaded gellan gum (GG) hydrogels were mineralized by incubation in mineralization media containing calcium and/or magnesium glycerophosphate (CaGP, MgGP).
24616374	2	21	contain	containing	422:431	arg1	media					416:420	mineralization media	401:420	mineralization media containing calcium and/or magnesium glycerophosphate (CaGP, MgGP)	401:486	ALP-loaded gellan gum (GG) hydrogels were mineralized by incubation in mineralization media containing calcium and/or magnesium glycerophosphate (CaGP, MgGP).
24616374	4	22	from	SAED	743:746	arg1	fraction					783:790	the mass fraction	774:790	the the mass fraction of the hydrogel not consisting of water	770:830	Mineral formation was confirmed by IR and Raman, SEM, ICP-OES, XRD, TEM, SAED, TGA and increases in the the mass fraction of the hydrogel not consisting of water.
24616374	4	23	from	TEM	738:740	arg1	fraction					783:790	the mass fraction	774:790	the the mass fraction of the hydrogel not consisting of water	770:830	Mineral formation was confirmed by IR and Raman, SEM, ICP-OES, XRD, TEM, SAED, TGA and increases in the the mass fraction of the hydrogel not consisting of water.
24616374	11	24	contain	containing	1584:1593	arg1	B-E					1579:1581	media B-E	1573:1581	media B-E (containing Mg)	1573:1597	The attachment and vitality of osteoblastic MC3T3-E1 cells were higher on samples mineralized in media B-E (containing Mg) than in those mineralized in medium A (not containing Mg).
24616374	11	24	contain	containing	1584:1593	arg2	Mg					1595:1596	Mg	1595:1596	Mg	1595:1596	The attachment and vitality of osteoblastic MC3T3-E1 cells were higher on samples mineralized in media B-E (containing Mg) than in those mineralized in medium A (not containing Mg).
24616374	9	25	dep	crystalline	1267:1277	arg1	MgP					1293:1295	MgP	1293:1295	MgP	1293:1295	Mineral formed in medium E was a combination of crystalline and amorphous MgP.
24616374	8	26	theme	medium	1186:1191	arg1	D					1193:1193	medium D	1186:1193	medium D	1186:1193	Mineral formed in medium D was an amorphous phase.
24616374	11	27	dep	A	1635:1635	arg1	containing					1642:1651	containing	1642:1651	containing Mg	1642:1654	The attachment and vitality of osteoblastic MC3T3-E1 cells were higher on samples mineralized in media B-E (containing Mg) than in those mineralized in medium A (not containing Mg).
24616374	4	28	from	XRD	733:735	arg1	fraction					783:790	the mass fraction	774:790	the the mass fraction of the hydrogel not consisting of water	770:830	Mineral formation was confirmed by IR and Raman, SEM, ICP-OES, XRD, TEM, SAED, TGA and increases in the the mass fraction of the hydrogel not consisting of water.
24616374	4	29	from	ICP-OES	724:730	arg1	fraction					783:790	the mass fraction	774:790	the the mass fraction of the hydrogel not consisting of water	770:830	Mineral formation was confirmed by IR and Raman, SEM, ICP-OES, XRD, TEM, SAED, TGA and increases in the the mass fraction of the hydrogel not consisting of water.
24616374	12	30	dep	media	1776:1780	arg1	A					1782:1782	A	1782:1782	A	1782:1782	All samples underwent degradation and supported the adhesion of RAW 264.7 monocytic cells, and samples mineralized in media A and B supported osteoclast-like cell formation.
24616374	12	30	dep	media	1776:1780	arg1	media					1776:1780	media A and B	1776:1788	media A and B	1776:1788	All samples underwent degradation and supported the adhesion of RAW 264.7 monocytic cells, and samples mineralized in media A and B supported osteoclast-like cell formation.
24616374	12	30	dep	media	1776:1780	arg1	B					1788:1788	B	1788:1788	B	1788:1788	All samples underwent degradation and supported the adhesion of RAW 264.7 monocytic cells, and samples mineralized in media A and B supported osteoclast-like cell formation.
24616374	8	31	theme	amorphous	1202:1210	arg1	phase					1212:1216	an amorphous phase	1199:1216	an amorphous phase	1199:1216	Mineral formed in medium D was an amorphous phase.
24616374	8	31	theme	amorphous	1202:1210	arg1	Mineral					1168:1174	Mineral	1168:1174	Mineral	1168:1174	Mineral formed in medium D was an amorphous phase.
24616374	4	32	from	SEM	719:721	arg1	fraction					783:790	the mass fraction	774:790	the the mass fraction of the hydrogel not consisting of water	770:830	Mineral formation was confirmed by IR and Raman, SEM, ICP-OES, XRD, TEM, SAED, TGA and increases in the the mass fraction of the hydrogel not consisting of water.
24616374	11	33	from	mineralized	1558:1568	arg1	B-E					1579:1581	media B-E	1573:1581	media B-E (containing Mg)	1573:1597	The attachment and vitality of osteoblastic MC3T3-E1 cells were higher on samples mineralized in media B-E (containing Mg) than in those mineralized in medium A (not containing Mg).
24616374	0	34	theme	calcium	118:124	arg1	phases					150:155	calcium and magnesium phosphate phases	118:155	calcium and magnesium phosphate phases	118:155	Generation of composites for bone tissue-engineering applications consisting of gellan gum hydrogels mineralized with calcium and magnesium phosphate phases by enzymatic means.
24616374	4	35	from	increases	757:765	arg1	fraction					783:790	the mass fraction	774:790	the the mass fraction of the hydrogel not consisting of water	770:830	Mineral formation was confirmed by IR and Raman, SEM, ICP-OES, XRD, TEM, SAED, TGA and increases in the the mass fraction of the hydrogel not consisting of water.
24616374	12	36	theme	RAW	1722:1724	arg1	cells					1742:1746	RAW 264.7 monocytic cells	1722:1746	RAW 264.7 monocytic cells	1722:1746	All samples underwent degradation and supported the adhesion of RAW 264.7 monocytic cells, and samples mineralized in media A and B supported osteoclast-like cell formation.
24616374	6	37	theme	mineral	961:967	arg1	content					934:940	Mg content	931:940	Mg content	931:940	Mg content and amorphicity of mineral formed increased in the order A < B < C < D. Mineral formed in media A and B was calcium-deficient hydroxyapatite (CDHA).
24616374	6	37	theme	mineral	961:967	arg1	amorphicity					946:956	amorphicity	946:956	amorphicity	946:956	Mg content and amorphicity of mineral formed increased in the order A < B < C < D. Mineral formed in media A and B was calcium-deficient hydroxyapatite (CDHA).
24616374	0	38	theme	gellan	80:85	arg1	gum					87:89	gellan gum	80:89	gellan gum	80:89	Generation of composites for bone tissue-engineering applications consisting of gellan gum hydrogels mineralized with calcium and magnesium phosphate phases by enzymatic means.
24616374	1	39	theme	alkaline	302:309	arg1	ALP					324:326	ALP	324:326	ALP	324:326	Mineralization of hydrogels, desirable for bone regeneration applications, may be achieved enzymatically by incorporation of alkaline phosphatase (ALP).
24616374	1	39	theme	alkaline	302:309	arg1	phosphatase					311:321	alkaline phosphatase	302:321	alkaline phosphatase (ALP)	302:327	Mineralization of hydrogels, desirable for bone regeneration applications, may be achieved enzymatically by incorporation of alkaline phosphatase (ALP).
24616374	12	40	theme	monocytic	1732:1740	arg1	cells					1742:1746	RAW 264.7 monocytic cells	1722:1746	RAW 264.7 monocytic cells	1722:1746	All samples underwent degradation and supported the adhesion of RAW 264.7 monocytic cells, and samples mineralized in media A and B supported osteoclast-like cell formation.
24616374	3	41	dep	concentrations	525:538	arg1	A					623:623	A	623:623	A	623:623	Mineralization media with CaGP:MgGP concentrations 0.1:0, 0.075:0.025, 0.05:0.05, 0.025:0.075 and 0:0.1 (all values mol/dm3 , denoted A, B, C, D and E, respectively) were compared.
24616374	3	41	dep	concentrations	525:538	arg1	values					598:603	all values mol/dm3	594:611	all values mol/dm3	594:611	Mineralization media with CaGP:MgGP concentrations 0.1:0, 0.075:0.025, 0.05:0.05, 0.025:0.075 and 0:0.1 (all values mol/dm3 , denoted A, B, C, D and E, respectively) were compared.
24616374	6	42	dep	formed	969:974	arg1	increased					976:984	increased	976:984	formed increased in the order A < B < C < D. Mineral formed in media A and B was calcium-deficient hydroxyapatite (CDHA)	969:1088	Mg content and amorphicity of mineral formed increased in the order A < B < C < D. Mineral formed in media A and B was calcium-deficient hydroxyapatite (CDHA).
24616374	0	43	theme	composites	14:23	arg1	Generation					0:9	Generation	0:9	Generation of composites for bone tissue-engineering applications consisting of gellan gum	0:89	Generation of composites for bone tissue-engineering applications consisting of gellan gum hydrogels mineralized with calcium and magnesium phosphate phases by enzymatic means.
24616374	2	44	dep	calcium	433:439	arg1	CaGP					476:479	CaGP	476:479	CaGP	476:479	ALP-loaded gellan gum (GG) hydrogels were mineralized by incubation in mineralization media containing calcium and/or magnesium glycerophosphate (CaGP, MgGP).
24616374	2	44	dep	calcium	433:439	arg1	MgGP					482:485	MgGP	482:485	MgGP	482:485	ALP-loaded gellan gum (GG) hydrogels were mineralized by incubation in mineralization media containing calcium and/or magnesium glycerophosphate (CaGP, MgGP).
24616374	0	45	theme	bone	29:32	arg1	applications					53:64	bone tissue-engineering applications	29:64	bone tissue-engineering applications consisting of gellan gum	29:89	Generation of composites for bone tissue-engineering applications consisting of gellan gum hydrogels mineralized with calcium and magnesium phosphate phases by enzymatic means.
24616374	7	46	theme	amorphous	1151:1159	arg1	phase					1161:1165	an amorphous phase	1148:1165	an amorphous phase	1148:1165	Mineral formed in medium C was a combination of CDHA and an amorphous phase.
24616374	10	47	theme	medium	1374:1379	arg1	dependence					1345:1354	dependence	1345:1354	dependence of mineralization medium	1345:1379	Young's moduli and storage moduli decreased in dependence of mineralization medium in the order A > B > C > D, but were significantly higher for samples mineralized in medium E.
24616374	12	48	from	media	1776:1780	arg1	mineralized					1761:1771	mineralized	1761:1771	mineralized	1761:1771	All samples underwent degradation and supported the adhesion of RAW 264.7 monocytic cells, and samples mineralized in media A and B supported osteoclast-like cell formation.
24616374	1	49	theme	hydrogels	195:203	arg1	Mineralization					177:190	Mineralization	177:190	Mineralization of hydrogels, desirable for bone regeneration applications,	177:250	Mineralization of hydrogels, desirable for bone regeneration applications, may be achieved enzymatically by incorporation of alkaline phosphatase (ALP).
24616374	10	50	from	mineralized	1451:1461	arg1	E					1473:1473	medium E	1466:1473	medium E	1466:1473	Young's moduli and storage moduli decreased in dependence of mineralization medium in the order A > B > C > D, but were significantly higher for samples mineralized in medium E.
24616374	11	51	theme	osteoblastic	1507:1518	arg1	cells					1529:1533	osteoblastic MC3T3-E1 cells	1507:1533	osteoblastic MC3T3-E1 cells	1507:1533	The attachment and vitality of osteoblastic MC3T3-E1 cells were higher on samples mineralized in media B-E (containing Mg) than in those mineralized in medium A (not containing Mg).
24616374	3	52	theme	MgGP	520:523	arg1	concentrations					525:538	MgGP concentrations 0.1:0, 0.075:0.025, 0.05:0.05, 0.025:0.075 and 0:0.1	520:591	MgGP concentrations 0.1:0, 0.075:0.025, 0.05:0.05, 0.025:0.075 and 0:0.1 (all values mol/dm3 , denoted A, B, C, D and E, respectively)	520:653	Mineralization media with CaGP:MgGP concentrations 0.1:0, 0.075:0.025, 0.05:0.05, 0.025:0.075 and 0:0.1 (all values mol/dm3 , denoted A, B, C, D and E, respectively) were compared.
24616374	3	53	theme	0.05:0.05	560:568	arg1	0.1:0					540:544	0.1:0	540:544	0.1:0	540:544	Mineralization media with CaGP:MgGP concentrations 0.1:0, 0.075:0.025, 0.05:0.05, 0.025:0.075 and 0:0.1 (all values mol/dm3 , denoted A, B, C, D and E, respectively) were compared.
24616374	3	54	with	media	504:508	arg1	CaGP					515:518	CaGP	515:518	CaGP	515:518	Mineralization media with CaGP:MgGP concentrations 0.1:0, 0.075:0.025, 0.05:0.05, 0.025:0.075 and 0:0.1 (all values mol/dm3 , denoted A, B, C, D and E, respectively) were compared.
24616374	3	55	theme	0.075:0.025	547:557	arg1	0.1:0					540:544	0.1:0	540:544	0.1:0	540:544	Mineralization media with CaGP:MgGP concentrations 0.1:0, 0.075:0.025, 0.05:0.05, 0.025:0.075 and 0:0.1 (all values mol/dm3 , denoted A, B, C, D and E, respectively) were compared.
24616374	6	56	dep	media	1032:1036	arg1	media					1032:1036	media A and B	1032:1044	media A and B was calcium-deficient hydroxyapatite (CDHA)	1032:1088	Mg content and amorphicity of mineral formed increased in the order A < B < C < D. Mineral formed in media A and B was calcium-deficient hydroxyapatite (CDHA).
24616374	6	56	dep	media	1032:1036	arg1	B					1044:1044	B	1044:1044	B	1044:1044	Mg content and amorphicity of mineral formed increased in the order A < B < C < D. Mineral formed in media A and B was calcium-deficient hydroxyapatite (CDHA).
24616374	6	56	dep	media	1032:1036	arg1	A					1038:1038	A	1038:1038	A	1038:1038	Mg content and amorphicity of mineral formed increased in the order A < B < C < D. Mineral formed in media A and B was calcium-deficient hydroxyapatite (CDHA).
24616374	10	57	theme	medium	1466:1471	arg1	E					1473:1473	medium E	1466:1473	medium E	1466:1473	Young's moduli and storage moduli decreased in dependence of mineralization medium in the order A > B > C > D, but were significantly higher for samples mineralized in medium E.
24616374	11	58	theme	cells	1529:1533	arg1	vitality					1495:1502	vitality	1495:1502	vitality	1495:1502	The attachment and vitality of osteoblastic MC3T3-E1 cells were higher on samples mineralized in media B-E (containing Mg) than in those mineralized in medium A (not containing Mg).
24616374	11	58	theme	cells	1529:1533	arg1	attachment					1480:1489	attachment	1480:1489	attachment	1480:1489	The attachment and vitality of osteoblastic MC3T3-E1 cells were higher on samples mineralized in media B-E (containing Mg) than in those mineralized in medium A (not containing Mg).
24616374	2	59	theme	gellan	341:346	arg1	GG					353:354	GG	353:354	GG	353:354	ALP-loaded gellan gum (GG) hydrogels were mineralized by incubation in mineralization media containing calcium and/or magnesium glycerophosphate (CaGP, MgGP).
24616374	2	59	theme	gellan	341:346	arg1	gum					348:350	ALP-loaded gellan gum	330:350	ALP-loaded gellan gum (GG) hydrogels	330:365	ALP-loaded gellan gum (GG) hydrogels were mineralized by incubation in mineralization media containing calcium and/or magnesium glycerophosphate (CaGP, MgGP).
24616374	5	60	from	Mg	891:892	arg1	samples					897:903	samples	897:903	samples mineralized in media A-D	897:928	Ca was incorporated into mineral to a greater extent than Mg in samples mineralized in media A-D.
24616374	10	61	theme	mineralized	1451:1461	arg1	samples					1443:1449	samples	1443:1449	samples mineralized in medium E	1443:1473	Young's moduli and storage moduli decreased in dependence of mineralization medium in the order A > B > C > D, but were significantly higher for samples mineralized in medium E.
24616374	0	62	theme	phosphate	140:148	arg1	phases					150:155	calcium and magnesium phosphate phases	118:155	calcium and magnesium phosphate phases	118:155	Generation of composites for bone tissue-engineering applications consisting of gellan gum hydrogels mineralized with calcium and magnesium phosphate phases by enzymatic means.
24616374	0	63	with	mineralized	101:111	arg1	phases					150:155	calcium and magnesium phosphate phases	118:155	calcium and magnesium phosphate phases	118:155	Generation of composites for bone tissue-engineering applications consisting of gellan gum hydrogels mineralized with calcium and magnesium phosphate phases by enzymatic means.
24616374	3	64	theme	0.025:0.075	571:581	arg1	0.1:0					540:544	0.1:0	540:544	0.1:0	540:544	Mineralization media with CaGP:MgGP concentrations 0.1:0, 0.075:0.025, 0.05:0.05, 0.025:0.075 and 0:0.1 (all values mol/dm3 , denoted A, B, C, D and E, respectively) were compared.
24616374	12	65	theme	osteoclast-like	1800:1814	arg1	formation					1821:1829	osteoclast-like cell formation	1800:1829	osteoclast-like cell formation	1800:1829	All samples underwent degradation and supported the adhesion of RAW 264.7 monocytic cells, and samples mineralized in media A and B supported osteoclast-like cell formation.
24616374	11	66	from	mineralized	1613:1623	arg1	A					1635:1635	medium A	1628:1635	medium A (not containing Mg)	1628:1655	The attachment and vitality of osteoblastic MC3T3-E1 cells were higher on samples mineralized in media B-E (containing Mg) than in those mineralized in medium A (not containing Mg).
24616374	1	67	theme	regeneration	225:236	arg1	applications					238:249	bone regeneration applications	220:249	bone regeneration applications	220:249	Mineralization of hydrogels, desirable for bone regeneration applications, may be achieved enzymatically by incorporation of alkaline phosphatase (ALP).
24616374	4	68	theme	Mineral	670:676	arg1	formation					678:686	Mineral formation	670:686	Mineral formation	670:686	Mineral formation was confirmed by IR and Raman, SEM, ICP-OES, XRD, TEM, SAED, TGA and increases in the the mass fraction of the hydrogel not consisting of water.
24616374	12	69	from	mineralized	1761:1771	arg1	A					1782:1782	A	1782:1782	A	1782:1782	All samples underwent degradation and supported the adhesion of RAW 264.7 monocytic cells, and samples mineralized in media A and B supported osteoclast-like cell formation.
24616374	12	69	from	mineralized	1761:1771	arg1	media					1776:1780	media A and B	1776:1788	media A and B	1776:1788	All samples underwent degradation and supported the adhesion of RAW 264.7 monocytic cells, and samples mineralized in media A and B supported osteoclast-like cell formation.
24616374	12	69	from	mineralized	1761:1771	arg1	B					1788:1788	B	1788:1788	B	1788:1788	All samples underwent degradation and supported the adhesion of RAW 264.7 monocytic cells, and samples mineralized in media A and B supported osteoclast-like cell formation.
24616374	7	70	theme	CDHA	1139:1142	arg1	combination					1124:1134	a combination	1122:1134	a combination of CDHA	1122:1142	Mineral formed in medium C was a combination of CDHA and an amorphous phase.
24616374	7	70	theme	CDHA	1139:1142	arg1	phase					1161:1165	an amorphous phase	1148:1165	an amorphous phase	1148:1165	Mineral formed in medium C was a combination of CDHA and an amorphous phase.
24616374	7	70	theme	CDHA	1139:1142	arg1	Mineral					1091:1097	Mineral	1091:1097	Mineral	1091:1097	Mineral formed in medium C was a combination of CDHA and an amorphous phase.
24616374	7	71	theme	medium	1109:1114	arg1	C					1116:1116	medium C	1109:1116	medium C	1109:1116	Mineral formed in medium C was a combination of CDHA and an amorphous phase.
24616374	11	72	from	B-E	1579:1581	arg1	mineralized					1558:1568	mineralized	1558:1568	mineralized	1558:1568	The attachment and vitality of osteoblastic MC3T3-E1 cells were higher on samples mineralized in media B-E (containing Mg) than in those mineralized in medium A (not containing Mg).
24616374	10	73	theme	Young	1298:1302	arg1	moduli					1306:1311	Young's moduli	1298:1311	Young's moduli	1298:1311	Young's moduli and storage moduli decreased in dependence of mineralization medium in the order A > B > C > D, but were significantly higher for samples mineralized in medium E.
24616374	11	74	theme	medium	1628:1633	arg1	A					1635:1635	medium A	1628:1635	medium A (not containing Mg)	1628:1655	The attachment and vitality of osteoblastic MC3T3-E1 cells were higher on samples mineralized in media B-E (containing Mg) than in those mineralized in medium A (not containing Mg).
24616374	11	75	dep	containing	1642:1651	arg1	not					1638:1640	not	1638:1640	not	1638:1640	The attachment and vitality of osteoblastic MC3T3-E1 cells were higher on samples mineralized in media B-E (containing Mg) than in those mineralized in medium A (not containing Mg).
24616374	12	76	theme	mineralized	1761:1771	arg1	samples					1753:1759	samples	1753:1759	samples mineralized in media A and B	1753:1788	All samples underwent degradation and supported the adhesion of RAW 264.7 monocytic cells, and samples mineralized in media A and B supported osteoclast-like cell formation.
24616374	3	77	dep	media	504:508	arg1	compared					660:667	compared	660:667	were compared	655:667	Mineralization media with CaGP:MgGP concentrations 0.1:0, 0.075:0.025, 0.05:0.05, 0.025:0.075 and 0:0.1 (all values mol/dm3 , denoted A, B, C, D and E, respectively) were compared.
24616374	5	78	theme	greater	871:877	arg1	extent					879:884	a greater extent	869:884	a greater extent than Mg in samples mineralized in media A-D	869:928	Ca was incorporated into mineral to a greater extent than Mg in samples mineralized in media A-D.
24616374	13	79	dep	&	1860:1860	arg1	Sons					1862:1865	Sons	1862:1865	Sons	1862:1865	Copyright © 2014 John Wiley & Sons, Ltd.
24616374	10	80	theme	storage	1317:1323	arg1	moduli					1325:1330	storage moduli	1317:1330	storage moduli	1317:1330	Young's moduli and storage moduli decreased in dependence of mineralization medium in the order A > B > C > D, but were significantly higher for samples mineralized in medium E.
24616374	2	81	theme	mineralization	401:414	arg1	media					416:420	mineralization media	401:420	mineralization media containing calcium and/or magnesium glycerophosphate (CaGP, MgGP)	401:486	ALP-loaded gellan gum (GG) hydrogels were mineralized by incubation in mineralization media containing calcium and/or magnesium glycerophosphate (CaGP, MgGP).
24616374	12	82	theme	cells	1742:1746	arg1	adhesion					1710:1717	the adhesion	1706:1717	the adhesion of RAW 264.7 monocytic cells	1706:1746	All samples underwent degradation and supported the adhesion of RAW 264.7 monocytic cells, and samples mineralized in media A and B supported osteoclast-like cell formation.
24616374	1	83	theme	phosphatase	311:321	arg1	incorporation					285:297	incorporation	285:297	incorporation of alkaline phosphatase (ALP)	285:327	Mineralization of hydrogels, desirable for bone regeneration applications, may be achieved enzymatically by incorporation of alkaline phosphatase (ALP).
24616374	2	84	theme	glycerophosphate	458:473	arg1	magnesium					448:456	magnesium glycerophosphate	448:473	magnesium glycerophosphate	448:473	ALP-loaded gellan gum (GG) hydrogels were mineralized by incubation in mineralization media containing calcium and/or magnesium glycerophosphate (CaGP, MgGP).
24616374	11	85	theme	mineralized	1558:1568	arg1	samples					1550:1556	samples	1550:1556	samples mineralized in media B-E (containing Mg)	1550:1597	The attachment and vitality of osteoblastic MC3T3-E1 cells were higher on samples mineralized in media B-E (containing Mg) than in those mineralized in medium A (not containing Mg).
24616374	3	86	theme	Mineralization	489:502	arg1	media					504:508	Mineralization media	489:508	Mineralization media with CaGP:MgGP concentrations 0.1:0, 0.075:0.025, 0.05:0.05, 0.025:0.075 and 0:0.1 (all values mol/dm3 , denoted A, B, C, D and E, respectively) were compared.	489:668	Mineralization media with CaGP:MgGP concentrations 0.1:0, 0.075:0.025, 0.05:0.05, 0.025:0.075 and 0:0.1 (all values mol/dm3 , denoted A, B, C, D and E, respectively) were compared.
24616374	0	87	theme	tissue-engineering	34:51	arg1	applications					53:64	bone tissue-engineering applications	29:64	bone tissue-engineering applications consisting of gellan gum	29:89	Generation of composites for bone tissue-engineering applications consisting of gellan gum hydrogels mineralized with calcium and magnesium phosphate phases by enzymatic means.
24616374	5	88	theme	mineralized	905:915	arg1	samples					897:903	samples	897:903	samples mineralized in media A-D	897:928	Ca was incorporated into mineral to a greater extent than Mg in samples mineralized in media A-D.
24616374	6	89	theme	Mg	931:932	arg1	content					934:940	Mg content	931:940	Mg content	931:940	Mg content and amorphicity of mineral formed increased in the order A < B < C < D. Mineral formed in media A and B was calcium-deficient hydroxyapatite (CDHA).
24616374	2	90	from	incubation	387:396	arg1	media					416:420	mineralization media	401:420	mineralization media containing calcium and/or magnesium glycerophosphate (CaGP, MgGP)	401:486	ALP-loaded gellan gum (GG) hydrogels were mineralized by incubation in mineralization media containing calcium and/or magnesium glycerophosphate (CaGP, MgGP).
24616374	9	91	theme	medium	1237:1242	arg1	E					1244:1244	medium E	1237:1244	medium E	1237:1244	Mineral formed in medium E was a combination of crystalline and amorphous MgP.
24616374	6	92	theme	Mineral	1014:1020	arg1	A < B < C < D.					999:1012	the order A < B < C < D. Mineral	989:1020	the order A < B < C < D. Mineral formed in media A and B was calcium-deficient hydroxyapatite (CDHA)	989:1088	Mg content and amorphicity of mineral formed increased in the order A < B < C < D. Mineral formed in media A and B was calcium-deficient hydroxyapatite (CDHA).
24616374	10	93	theme	mineralization	1359:1372	arg1	medium					1374:1379	mineralization medium	1359:1379	mineralization medium	1359:1379	Young's moduli and storage moduli decreased in dependence of mineralization medium in the order A > B > C > D, but were significantly higher for samples mineralized in medium E.
24616374	11	94	theme	media	1573:1577	arg1	B-E					1579:1581	media B-E	1573:1581	media B-E (containing Mg)	1573:1597	The attachment and vitality of osteoblastic MC3T3-E1 cells were higher on samples mineralized in media B-E (containing Mg) than in those mineralized in medium A (not containing Mg).
24616374	2	95	theme	gum	348:350	arg1	hydrogels					357:365	ALP-loaded gellan gum (GG) hydrogels	330:365	ALP-loaded gellan gum (GG) hydrogels	330:365	ALP-loaded gellan gum (GG) hydrogels were mineralized by incubation in mineralization media containing calcium and/or magnesium glycerophosphate (CaGP, MgGP).
24616374	6	96	theme	order	993:997	arg1	A < B < C < D.					999:1012	the order A < B < C < D. Mineral	989:1020	the order A < B < C < D. Mineral formed in media A and B was calcium-deficient hydroxyapatite (CDHA)	989:1088	Mg content and amorphicity of mineral formed increased in the order A < B < C < D. Mineral formed in media A and B was calcium-deficient hydroxyapatite (CDHA).
28521987	8	0	theme	viscoelastic	1136:1147	arg1	features					1149:1156	their tensile and viscoelastic features	1118:1156	their tensile and viscoelastic features	1118:1156	An additional UV curing treatment was conducted on the pectin/coffee films with the aim to improve their tensile and viscoelastic features.
28521987	10	1	theme	coffee	1523:1528	arg1	clusters					1530:1537	coffee clusters	1523:1537	coffee clusters	1523:1537	In particular, the presence of single coffee particles into the pectin matrix renders the UV curing treatment effective in the enhancement of the elasticity as well as the traction resistance, whereas the cured composite films containing coffee clusters showed only more elastic characteristics.
28521987	8	2	theme	additional	1022:1031	arg1	treatment					1043:1051	An additional UV curing treatment	1019:1051	An additional UV curing treatment	1019:1051	An additional UV curing treatment was conducted on the pectin/coffee films with the aim to improve their tensile and viscoelastic features.
28521987	9	3	theme	cured	1163:1167	arg1	films					1169:1173	The cured films	1159:1173	The cured films	1159:1173	The cured films showed promising and tunable properties that are dependent on both the filler content and the UV irradiation.
28521987	3	4	theme	particles	492:500	arg1	structure					441:449	their mesoscopic structure	424:449	their mesoscopic structure as well as the sizes distribution of the particles dispersed into the matrix	424:526	The morphology of the pectin/coffee hybrid films was studied by microscopic techniques in order to investigate their mesoscopic structure as well as the sizes distribution of the particles dispersed into the matrix.
28521987	3	4	theme	particles	492:500	arg1	distribution					472:483	the sizes distribution	462:483	their mesoscopic structure as well as the sizes distribution of the particles dispersed into the matrix	424:526	The morphology of the pectin/coffee hybrid films was studied by microscopic techniques in order to investigate their mesoscopic structure as well as the sizes distribution of the particles dispersed into the matrix.
28521987	0	5	theme	competitive	59:69	arg1	performances					71:82	competitive performances	59:82	competitive performances dependent on the UV irradiation	59:114	Coffee grounds as filler for pectin: Green composites with competitive performances dependent on the UV irradiation.
28521987	10	6	theme	curing	1378:1383	arg1	treatment					1385:1393	the UV curing treatment	1371:1393	the UV curing treatment	1371:1393	In particular, the presence of single coffee particles into the pectin matrix renders the UV curing treatment effective in the enhancement of the elasticity as well as the traction resistance, whereas the cured composite films containing coffee clusters showed only more elastic characteristics.
28521987	8	7	theme	UV	1033:1034	arg1	treatment					1043:1051	An additional UV curing treatment	1019:1051	An additional UV curing treatment	1019:1051	An additional UV curing treatment was conducted on the pectin/coffee films with the aim to improve their tensile and viscoelastic features.
28521987	10	8	theme	elasticity	1431:1440	arg1	enhancement					1412:1422	the enhancement	1408:1422	the enhancement of the elasticity as well as the traction resistance	1408:1475	In particular, the presence of single coffee particles into the pectin matrix renders the UV curing treatment effective in the enhancement of the elasticity as well as the traction resistance, whereas the cured composite films containing coffee clusters showed only more elastic characteristics.
28521987	10	8	theme	elasticity	1431:1440	arg1	resistance					1466:1475	the traction resistance	1453:1475	the enhancement of the elasticity as well as the traction resistance	1408:1475	In particular, the presence of single coffee particles into the pectin matrix renders the UV curing treatment effective in the enhancement of the elasticity as well as the traction resistance, whereas the cured composite films containing coffee clusters showed only more elastic characteristics.
28521987	3	9	theme	hybrid	349:354	arg1	films					356:360	the pectin/coffee hybrid films	331:360	the pectin/coffee hybrid films	331:360	The morphology of the pectin/coffee hybrid films was studied by microscopic techniques in order to investigate their mesoscopic structure as well as the sizes distribution of the particles dispersed into the matrix.
28521987	4	10	theme	polymeric	611:619	arg1	matrix					621:626	the polymeric matrix	607:626	the polymeric matrix	607:626	The micrographs showed that the coffee grounds are uniformly dispersed within the polymeric matrix.
28521987	5	11	theme	biocomposite	670:681	arg1	films					683:687	the biocomposite films	666:687	the biocomposite films	666:687	The morphological characteristics of the biocomposite films were correlated to their properties, such as wettability, water uptake, thermal behavior and mechanical performances.
28521987	10	12	theme	UV	1375:1376	arg1	treatment					1385:1393	the UV curing treatment	1371:1393	the UV curing treatment	1371:1393	In particular, the presence of single coffee particles into the pectin matrix renders the UV curing treatment effective in the enhancement of the elasticity as well as the traction resistance, whereas the cured composite films containing coffee clusters showed only more elastic characteristics.
28521987	10	13	contain	containing	1512:1521	arg2	clusters					1530:1537	coffee clusters	1523:1537	coffee clusters	1523:1537	In particular, the presence of single coffee particles into the pectin matrix renders the UV curing treatment effective in the enhancement of the elasticity as well as the traction resistance, whereas the cured composite films containing coffee clusters showed only more elastic characteristics.
28521987	10	13	contain	containing	1512:1521	arg1	films					1506:1510	the cured composite films	1486:1510	the cured composite films containing coffee clusters	1486:1537	In particular, the presence of single coffee particles into the pectin matrix renders the UV curing treatment effective in the enhancement of the elasticity as well as the traction resistance, whereas the cured composite films containing coffee clusters showed only more elastic characteristics.
28521987	3	14	theme	films	356:360	arg1	morphology					317:326	The morphology	313:326	The morphology of the pectin/coffee hybrid films	313:360	The morphology of the pectin/coffee hybrid films was studied by microscopic techniques in order to investigate their mesoscopic structure as well as the sizes distribution of the particles dispersed into the matrix.
28521987	11	15	theme	specific	1677:1684	arg1	application					1686:1696	specific application	1677:1696	specific application within the food packaging	1677:1722	With this study, we fabricated pectin/coffee bioplastics with controlled behavior appealing for specific application within the food packaging.
28521987	5	16	theme	films	683:687	arg1	characteristics					647:661	The morphological characteristics	629:661	The morphological characteristics of the biocomposite films	629:687	The morphological characteristics of the biocomposite films were correlated to their properties, such as wettability, water uptake, thermal behavior and mechanical performances.
28521987	9	17	theme	promising	1182:1190	arg1	properties					1204:1213	promising and tunable properties	1182:1213	promising and tunable properties that are dependent on both the filler content and the UV irradiation	1182:1282	The cured films showed promising and tunable properties that are dependent on both the filler content and the UV irradiation.
28521987	11	18	theme	pectin/coffee	1612:1624	arg1	bioplastics					1626:1636	pectin/coffee bioplastics	1612:1636	pectin/coffee bioplastics	1612:1636	With this study, we fabricated pectin/coffee bioplastics with controlled behavior appealing for specific application within the food packaging.
28521987	0	19	theme	UV	101:102	arg1	irradiation					104:114	the UV irradiation	97:114	the UV irradiation	97:114	Coffee grounds as filler for pectin: Green composites with competitive performances dependent on the UV irradiation.
28521987	0	20	with	composites	43:52	arg1	performances					71:82	competitive performances	59:82	competitive performances dependent on the UV irradiation	59:114	Coffee grounds as filler for pectin: Green composites with competitive performances dependent on the UV irradiation.
28521987	3	21	theme	mesoscopic	430:439	arg1	structure					441:449	their mesoscopic structure	424:449	their mesoscopic structure as well as the sizes distribution of the particles dispersed into the matrix	424:526	The morphology of the pectin/coffee hybrid films was studied by microscopic techniques in order to investigate their mesoscopic structure as well as the sizes distribution of the particles dispersed into the matrix.
28521987	3	22	theme	pectin/coffee	335:347	arg1	films					356:360	the pectin/coffee hybrid films	331:360	the pectin/coffee hybrid films	331:360	The morphology of the pectin/coffee hybrid films was studied by microscopic techniques in order to investigate their mesoscopic structure as well as the sizes distribution of the particles dispersed into the matrix.
28521987	6	23	theme	Dynamic	807:813	arg1	function					851:858	a function	849:858	a function of the humidity conditions	849:885	Dynamic mechanical test were conducted as a function of the humidity conditions.
28521987	6	23	theme	Dynamic	807:813	arg1	test					826:829	Dynamic mechanical test	807:829	Dynamic mechanical test	807:829	Dynamic mechanical test were conducted as a function of the humidity conditions.
28521987	3	24	theme	microscopic	377:387	arg1	techniques					389:398	microscopic techniques	377:398	microscopic techniques	377:398	The morphology of the pectin/coffee hybrid films was studied by microscopic techniques in order to investigate their mesoscopic structure as well as the sizes distribution of the particles dispersed into the matrix.
28521987	5	25	theme	water	747:751	arg1	uptake					753:758	water uptake	747:758	water uptake	747:758	The morphological characteristics of the biocomposite films were correlated to their properties, such as wettability, water uptake, thermal behavior and mechanical performances.
28521987	9	26	theme	tunable	1196:1202	arg1	properties					1204:1213	promising and tunable properties	1182:1213	promising and tunable properties that are dependent on both the filler content and the UV irradiation	1182:1282	The cured films showed promising and tunable properties that are dependent on both the filler content and the UV irradiation.
28521987	5	27	theme	morphological	633:645	arg1	characteristics					647:661	The morphological characteristics	629:661	The morphological characteristics of the biocomposite films	629:687	The morphological characteristics of the biocomposite films were correlated to their properties, such as wettability, water uptake, thermal behavior and mechanical performances.
28521987	1	28	theme	pectin	183:188	arg1	matrix					190:195	pectin matrix	183:195	pectin matrix	183:195	Novel composite bioplastics were successfully prepared by filling pectin matrix with treated coffee grounds.
28521987	0	29	theme	Coffee	0:5	arg1	grounds					7:13	Coffee grounds	0:13	Coffee grounds as filler for pectin: Green composites with competitive performances dependent on the UV irradiation.	0:115	Coffee grounds as filler for pectin: Green composites with competitive performances dependent on the UV irradiation.
28521987	2	30	theme	filler	299:304	arg1	range					306:310	a wide filler range	292:310	a wide filler range	292:310	The amount of coffee dispersed into the pectin was changed within a wide filler range.
28521987	10	31	from	enhancement	1412:1422	arg1	effective					1395:1403	effective	1395:1403	effective	1395:1403	In particular, the presence of single coffee particles into the pectin matrix renders the UV curing treatment effective in the enhancement of the elasticity as well as the traction resistance, whereas the cured composite films containing coffee clusters showed only more elastic characteristics.
28521987	8	32	theme	pectin/coffee	1074:1086	arg1	films					1088:1092	the pectin/coffee films	1070:1092	the pectin/coffee films with the aim to improve their tensile and viscoelastic features	1070:1156	An additional UV curing treatment was conducted on the pectin/coffee films with the aim to improve their tensile and viscoelastic features.
28521987	6	33	theme	mechanical	815:824	arg1	function					851:858	a function	849:858	a function of the humidity conditions	849:885	Dynamic mechanical test were conducted as a function of the humidity conditions.
28521987	6	33	theme	mechanical	815:824	arg1	test					826:829	Dynamic mechanical test	807:829	Dynamic mechanical test	807:829	Dynamic mechanical test were conducted as a function of the humidity conditions.
28521987	2	34	theme	wide	294:297	arg1	range					306:310	a wide filler range	292:310	a wide filler range	292:310	The amount of coffee dispersed into the pectin was changed within a wide filler range.
28521987	10	35	theme	pectin	1349:1354	arg1	matrix					1356:1361	the pectin matrix	1345:1361	the pectin matrix	1345:1361	In particular, the presence of single coffee particles into the pectin matrix renders the UV curing treatment effective in the enhancement of the elasticity as well as the traction resistance, whereas the cured composite films containing coffee clusters showed only more elastic characteristics.
28521987	4	36	theme	coffee	561:566	arg1	grounds					568:574	the coffee grounds	557:574	the coffee grounds	557:574	The micrographs showed that the coffee grounds are uniformly dispersed within the polymeric matrix.
28521987	11	37	theme	food	1709:1712	arg1	packaging					1714:1722	the food packaging	1705:1722	the food packaging	1705:1722	With this study, we fabricated pectin/coffee bioplastics with controlled behavior appealing for specific application within the food packaging.
28521987	1	38	theme	treated	202:208	arg1	grounds					217:223	treated coffee grounds	202:223	treated coffee grounds	202:223	Novel composite bioplastics were successfully prepared by filling pectin matrix with treated coffee grounds.
28521987	5	39	theme	mechanical	782:791	arg1	performances					793:804	mechanical performances	782:804	mechanical performances	782:804	The morphological characteristics of the biocomposite films were correlated to their properties, such as wettability, water uptake, thermal behavior and mechanical performances.
28521987	6	40	theme	conditions	876:885	arg1	function					851:858	a function	849:858	a function of the humidity conditions	849:885	Dynamic mechanical test were conducted as a function of the humidity conditions.
28521987	6	40	theme	conditions	876:885	arg1	test					826:829	Dynamic mechanical test	807:829	Dynamic mechanical test	807:829	Dynamic mechanical test were conducted as a function of the humidity conditions.
28521987	1	41	theme	coffee	210:215	arg1	grounds					217:223	treated coffee grounds	202:223	treated coffee grounds	202:223	Novel composite bioplastics were successfully prepared by filling pectin matrix with treated coffee grounds.
28521987	10	42	theme	traction	1457:1464	arg1	resistance					1466:1475	the traction resistance	1453:1475	the enhancement of the elasticity as well as the traction resistance	1408:1475	In particular, the presence of single coffee particles into the pectin matrix renders the UV curing treatment effective in the enhancement of the elasticity as well as the traction resistance, whereas the cured composite films containing coffee clusters showed only more elastic characteristics.
28521987	6	43	theme	humidity	867:874	arg1	conditions					876:885	the humidity conditions	863:885	the humidity conditions	863:885	Dynamic mechanical test were conducted as a function of the humidity conditions.
28521987	10	44	theme	particles	1330:1338	arg1	presence					1304:1311	the presence	1300:1311	the presence of single coffee particles into the pectin matrix	1300:1361	In particular, the presence of single coffee particles into the pectin matrix renders the UV curing treatment effective in the enhancement of the elasticity as well as the traction resistance, whereas the cured composite films containing coffee clusters showed only more elastic characteristics.
28521987	0	45	theme	Green	37:41	arg1	composites					43:52	Green composites	37:52	Coffee grounds as filler for pectin: Green composites with competitive performances dependent on the UV irradiation.	0:115	Coffee grounds as filler for pectin: Green composites with competitive performances dependent on the UV irradiation.
28521987	10	46	theme	coffee	1323:1328	arg1	particles					1330:1338	single coffee particles	1316:1338	single coffee particles	1316:1338	In particular, the presence of single coffee particles into the pectin matrix renders the UV curing treatment effective in the enhancement of the elasticity as well as the traction resistance, whereas the cured composite films containing coffee clusters showed only more elastic characteristics.
28521987	11	47	theme	controlled	1643:1652	arg1	behavior					1654:1661	controlled behavior	1643:1661	controlled behavior appealing for specific application within the food packaging	1643:1722	With this study, we fabricated pectin/coffee bioplastics with controlled behavior appealing for specific application within the food packaging.
28521987	10	48	theme	composite	1496:1504	arg1	films					1506:1510	the cured composite films	1486:1510	the cured composite films containing coffee clusters	1486:1537	In particular, the presence of single coffee particles into the pectin matrix renders the UV curing treatment effective in the enhancement of the elasticity as well as the traction resistance, whereas the cured composite films containing coffee clusters showed only more elastic characteristics.
28521987	9	49	theme	filler	1246:1251	arg1	content					1253:1259	the filler content	1242:1259	the filler content	1242:1259	The cured films showed promising and tunable properties that are dependent on both the filler content and the UV irradiation.
28521987	10	50	theme	single	1316:1321	arg1	particles					1330:1338	single coffee particles	1316:1338	single coffee particles	1316:1338	In particular, the presence of single coffee particles into the pectin matrix renders the UV curing treatment effective in the enhancement of the elasticity as well as the traction resistance, whereas the cured composite films containing coffee clusters showed only more elastic characteristics.
28521987	8	51	theme	curing	1036:1041	arg1	treatment					1043:1051	An additional UV curing treatment	1019:1051	An additional UV curing treatment	1019:1051	An additional UV curing treatment was conducted on the pectin/coffee films with the aim to improve their tensile and viscoelastic features.
28521987	7	52	theme	mechanical	928:937	arg1	performances					939:950	the mechanical performances	924:950	the mechanical performances	924:950	As a general result, a worsening of the mechanical performances was induced by the addition of the coffee grounds into the pectin.
28521987	10	53	theme	cured	1490:1494	arg1	films					1506:1510	the cured composite films	1486:1510	the cured composite films containing coffee clusters	1486:1537	In particular, the presence of single coffee particles into the pectin matrix renders the UV curing treatment effective in the enhancement of the elasticity as well as the traction resistance, whereas the cured composite films containing coffee clusters showed only more elastic characteristics.
28521987	7	54	theme	performances	939:950	arg1	result					901:906	a general result	891:906	a general result	891:906	As a general result, a worsening of the mechanical performances was induced by the addition of the coffee grounds into the pectin.
28521987	7	54	theme	performances	939:950	arg1	worsening					911:919	a worsening	909:919	a worsening of the mechanical performances	909:950	As a general result, a worsening of the mechanical performances was induced by the addition of the coffee grounds into the pectin.
28521987	10	55	theme	elastic	1556:1562	arg1	characteristics					1564:1578	only more elastic characteristics	1546:1578	only more elastic characteristics	1546:1578	In particular, the presence of single coffee particles into the pectin matrix renders the UV curing treatment effective in the enhancement of the elasticity as well as the traction resistance, whereas the cured composite films containing coffee clusters showed only more elastic characteristics.
28521987	1	56	theme	Novel	117:121	arg1	bioplastics					133:143	Novel composite bioplastics	117:143	Novel composite bioplastics	117:143	Novel composite bioplastics were successfully prepared by filling pectin matrix with treated coffee grounds.
28521987	8	57	with	films	1088:1092	arg1	aim					1103:1105	the aim to improve their tensile and viscoelastic features	1099:1156	the aim to improve their tensile and viscoelastic features	1099:1156	An additional UV curing treatment was conducted on the pectin/coffee films with the aim to improve their tensile and viscoelastic features.
28521987	10	58	from	resistance	1466:1475	arg1	effective					1395:1403	effective	1395:1403	effective	1395:1403	In particular, the presence of single coffee particles into the pectin matrix renders the UV curing treatment effective in the enhancement of the elasticity as well as the traction resistance, whereas the cured composite films containing coffee clusters showed only more elastic characteristics.
28521987	7	59	theme	coffee	987:992	arg1	grounds					994:1000	the coffee grounds	983:1000	the coffee grounds	983:1000	As a general result, a worsening of the mechanical performances was induced by the addition of the coffee grounds into the pectin.
28521987	1	60	theme	composite	123:131	arg1	bioplastics					133:143	Novel composite bioplastics	117:143	Novel composite bioplastics	117:143	Novel composite bioplastics were successfully prepared by filling pectin matrix with treated coffee grounds.
28521987	3	61	theme	sizes	466:470	arg1	distribution					472:483	the sizes distribution	462:483	their mesoscopic structure as well as the sizes distribution of the particles dispersed into the matrix	424:526	The morphology of the pectin/coffee hybrid films was studied by microscopic techniques in order to investigate their mesoscopic structure as well as the sizes distribution of the particles dispersed into the matrix.
28521987	8	62	theme	tensile	1124:1130	arg1	features					1149:1156	their tensile and viscoelastic features	1118:1156	their tensile and viscoelastic features	1118:1156	An additional UV curing treatment was conducted on the pectin/coffee films with the aim to improve their tensile and viscoelastic features.
28521987	0	63	dep	grounds	7:13	arg1	composites					43:52	Green composites	37:52	Coffee grounds as filler for pectin: Green composites with competitive performances dependent on the UV irradiation.	0:115	Coffee grounds as filler for pectin: Green composites with competitive performances dependent on the UV irradiation.
28521987	7	64	theme	grounds	994:1000	arg1	addition					971:978	the addition	967:978	the addition of the coffee grounds into the pectin	967:1016	As a general result, a worsening of the mechanical performances was induced by the addition of the coffee grounds into the pectin.
28521987	10	65	from	effective	1395:1403	arg1	enhancement					1412:1422	the enhancement	1408:1422	the enhancement of the elasticity as well as the traction resistance	1408:1475	In particular, the presence of single coffee particles into the pectin matrix renders the UV curing treatment effective in the enhancement of the elasticity as well as the traction resistance, whereas the cured composite films containing coffee clusters showed only more elastic characteristics.
28521987	10	65	from	effective	1395:1403	arg1	resistance					1466:1475	the traction resistance	1453:1475	the enhancement of the elasticity as well as the traction resistance	1408:1475	In particular, the presence of single coffee particles into the pectin matrix renders the UV curing treatment effective in the enhancement of the elasticity as well as the traction resistance, whereas the cured composite films containing coffee clusters showed only more elastic characteristics.
28521987	5	66	theme	thermal	761:767	arg1	behavior					769:776	thermal behavior	761:776	thermal behavior	761:776	The morphological characteristics of the biocomposite films were correlated to their properties, such as wettability, water uptake, thermal behavior and mechanical performances.
28521987	9	67	theme	UV	1269:1270	arg1	irradiation					1272:1282	the UV irradiation	1265:1282	the UV irradiation	1265:1282	The cured films showed promising and tunable properties that are dependent on both the filler content and the UV irradiation.
28521987	2	68	theme	coffee	240:245	arg1	amount					230:235	The amount	226:235	The amount of coffee dispersed into the pectin	226:271	The amount of coffee dispersed into the pectin was changed within a wide filler range.
28521987	2	68	theme	coffee	240:245	arg1	coffee					240:245	coffee	240:245	coffee dispersed into the pectin	240:271	The amount of coffee dispersed into the pectin was changed within a wide filler range.
28521987	7	69	theme	general	893:899	arg1	result					901:906	a general result	891:906	a general result	891:906	As a general result, a worsening of the mechanical performances was induced by the addition of the coffee grounds into the pectin.
28521987	7	69	theme	general	893:899	arg1	worsening					911:919	a worsening	909:919	a worsening of the mechanical performances	909:950	As a general result, a worsening of the mechanical performances was induced by the addition of the coffee grounds into the pectin.
28521987	0	70	theme	dependent	84:92	arg1	performances					71:82	competitive performances	59:82	competitive performances dependent on the UV irradiation	59:114	Coffee grounds as filler for pectin: Green composites with competitive performances dependent on the UV irradiation.
26356251	2	0	from	step	480:483	arg1	process					508:514	the internalization process	488:514	the internalization process	488:514	We hypothesize that the electrostatic interaction between the positively charged amines of the cell-penetrating compounds and the negatively charged glycosaminoglycans (GAGs) extending from cell surfaces is the initiating step in the internalization process.
26356251	6	1	theme	model	1281:1285	arg1	membranes					1294:1302	model plasma membranes	1281:1302	model plasma membranes	1281:1302	These results were compared to the association of CPCs with lipid vesicles of varying composition as model plasma membranes to finally clarify the relative importance of each cell surface component in initial cell recognition.
26356251	6	2	theme	plasma	1287:1292	arg1	membranes					1294:1302	model plasma membranes	1281:1302	model plasma membranes	1281:1302	These results were compared to the association of CPCs with lipid vesicles of varying composition as model plasma membranes to finally clarify the relative importance of each cell surface component in initial cell recognition.
26356251	3	3	theme	dendrimer	556:564	arg1	interactions					521:532	The interactions	517:532	The interactions of generation 5 PAMAM dendrimer, Tat peptide and 25 kDa linear PEI with four different GAGs	517:624	The interactions of generation 5 PAMAM dendrimer, Tat peptide and 25 kDa linear PEI with four different GAGs have been studied using isothermal titration calorimetry to elucidate structure-function relationships that could lead to improved drug and gene delivery methods to a wide variety of cell types.
26356251	3	4	theme	gene	766:769	arg1	methods					780:786	gene delivery methods	766:786	gene delivery methods	766:786	The interactions of generation 5 PAMAM dendrimer, Tat peptide and 25 kDa linear PEI with four different GAGs have been studied using isothermal titration calorimetry to elucidate structure-function relationships that could lead to improved drug and gene delivery methods to a wide variety of cell types.
26356251	3	5	theme	improved	748:755	arg1	drug					757:760	improved drug	748:760	improved drug	748:760	The interactions of generation 5 PAMAM dendrimer, Tat peptide and 25 kDa linear PEI with four different GAGs have been studied using isothermal titration calorimetry to elucidate structure-function relationships that could lead to improved drug and gene delivery methods to a wide variety of cell types.
26356251	2	6	theme	charged	399:405	arg1	glycosaminoglycans					407:424	the negatively charged glycosaminoglycans	384:424	the negatively charged glycosaminoglycans (GAGs) extending from cell surfaces	384:460	We hypothesize that the electrostatic interaction between the positively charged amines of the cell-penetrating compounds and the negatively charged glycosaminoglycans (GAGs) extending from cell surfaces is the initiating step in the internalization process.
26356251	2	6	theme	charged	399:405	arg1	GAGs					427:430	GAGs	427:430	GAGs	427:430	We hypothesize that the electrostatic interaction between the positively charged amines of the cell-penetrating compounds and the negatively charged glycosaminoglycans (GAGs) extending from cell surfaces is the initiating step in the internalization process.
26356251	6	7	from	importance	1336:1345	arg1	recognition					1394:1404	initial cell recognition	1381:1404	initial cell recognition	1381:1404	These results were compared to the association of CPCs with lipid vesicles of varying composition as model plasma membranes to finally clarify the relative importance of each cell surface component in initial cell recognition.
26356251	6	8	theme	surface	1360:1366	arg1	component					1368:1376	each cell surface component	1350:1376	each cell surface component	1350:1376	These results were compared to the association of CPCs with lipid vesicles of varying composition as model plasma membranes to finally clarify the relative importance of each cell surface component in initial cell recognition.
26356251	2	9	theme	charged	331:337	arg1	amines					339:344	the positively charged amines	316:344	the positively charged amines of the cell-penetrating compounds	316:378	We hypothesize that the electrostatic interaction between the positively charged amines of the cell-penetrating compounds and the negatively charged glycosaminoglycans (GAGs) extending from cell surfaces is the initiating step in the internalization process.
26356251	6	10	theme	CPCs	1230:1233	arg1	association					1215:1225	the association	1211:1225	the association of CPCs with lipid vesicles of varying composition as model plasma membranes to finally clarify the relative importance of each cell surface component in initial cell recognition	1211:1404	These results were compared to the association of CPCs with lipid vesicles of varying composition as model plasma membranes to finally clarify the relative importance of each cell surface component in initial cell recognition.
26356251	0	11	theme	density-	106:113	arg1	manner					145:150	a charge density- and stereochemistry-dependent manner	97:150	a charge density- and stereochemistry-dependent manner	97:150	Cell-penetrating compounds preferentially bind glycosaminoglycans over plasma membrane lipids in a charge density- and stereochemistry-dependent manner.
26356251	7	12	theme	lipid	1449:1453	arg1	content					1455:1461	anionic lipid content	1441:1461	anionic lipid content	1441:1461	CPC-lipid affinity increases with anionic lipid content, but GAG affinity is higher for all cell-penetrating compounds, confirming the role these heterogeneous polysaccharides play in cellular association and clustering.
26356251	3	13	theme	linear	590:595	arg1	PEI					597:599	25 kDa linear PEI	583:599	25 kDa linear PEI	583:599	The interactions of generation 5 PAMAM dendrimer, Tat peptide and 25 kDa linear PEI with four different GAGs have been studied using isothermal titration calorimetry to elucidate structure-function relationships that could lead to improved drug and gene delivery methods to a wide variety of cell types.
26356251	3	14	theme	generation	537:546	arg1	dendrimer					556:564	generation 5 PAMAM dendrimer	537:564	generation 5 PAMAM dendrimer	537:564	The interactions of generation 5 PAMAM dendrimer, Tat peptide and 25 kDa linear PEI with four different GAGs have been studied using isothermal titration calorimetry to elucidate structure-function relationships that could lead to improved drug and gene delivery methods to a wide variety of cell types.
26356251	5	15	theme	hydrogen	1098:1105	arg1	bonding					1107:1113	hydrogen bonding	1098:1113	hydrogen bonding between CPC amines and amides and GAG hydroxyl and amine groups	1098:1177	The effect of GAG composition on affinity is likely due to hydrogen bonding between CPC amines and amides and GAG hydroxyl and amine groups.
26356251	4	16	theme	thermodynamic	830:842	arg1	analysis					844:851	Detailed thermodynamic analysis	821:851	Detailed thermodynamic analysis	821:851	Detailed thermodynamic analysis has determined that CPC-GAG binding constants range from 8.7×10(3) to 2.4×10(6)M(-1) and that affinity is dependent upon GAG charge density and stereochemistry and CPC molecular weight.
26356251	2	17	theme	cell	448:451	arg1	surfaces					453:460	cell surfaces	448:460	cell surfaces	448:460	We hypothesize that the electrostatic interaction between the positively charged amines of the cell-penetrating compounds and the negatively charged glycosaminoglycans (GAGs) extending from cell surfaces is the initiating step in the internalization process.
26356251	0	18	theme	stereochemistry-dependent	119:143	arg1	manner					145:150	a charge density- and stereochemistry-dependent manner	97:150	a charge density- and stereochemistry-dependent manner	97:150	Cell-penetrating compounds preferentially bind glycosaminoglycans over plasma membrane lipids in a charge density- and stereochemistry-dependent manner.
26356251	3	19	theme	delivery	771:778	arg1	methods					780:786	gene delivery methods	766:786	gene delivery methods	766:786	The interactions of generation 5 PAMAM dendrimer, Tat peptide and 25 kDa linear PEI with four different GAGs have been studied using isothermal titration calorimetry to elucidate structure-function relationships that could lead to improved drug and gene delivery methods to a wide variety of cell types.
26356251	3	20	theme	25	583:584	arg1	kDa					586:588	kDa	586:588	kDa	586:588	The interactions of generation 5 PAMAM dendrimer, Tat peptide and 25 kDa linear PEI with four different GAGs have been studied using isothermal titration calorimetry to elucidate structure-function relationships that could lead to improved drug and gene delivery methods to a wide variety of cell types.
26356251	7	21	theme	CPC-lipid	1407:1415	arg1	affinity					1417:1424	CPC-lipid affinity	1407:1424	CPC-lipid affinity	1407:1424	CPC-lipid affinity increases with anionic lipid content, but GAG affinity is higher for all cell-penetrating compounds, confirming the role these heterogeneous polysaccharides play in cellular association and clustering.
26356251	2	22	theme	internalization	492:506	arg1	process					508:514	the internalization process	488:514	the internalization process	488:514	We hypothesize that the electrostatic interaction between the positively charged amines of the cell-penetrating compounds and the negatively charged glycosaminoglycans (GAGs) extending from cell surfaces is the initiating step in the internalization process.
26356251	6	23	theme	component	1368:1376	arg1	importance					1336:1345	the relative importance	1323:1345	the relative importance of each cell surface component in initial cell recognition	1323:1404	These results were compared to the association of CPCs with lipid vesicles of varying composition as model plasma membranes to finally clarify the relative importance of each cell surface component in initial cell recognition.
26356251	6	24	theme	lipid	1240:1244	arg1	vesicles					1246:1253	lipid vesicles	1240:1253	lipid vesicles of varying composition as model plasma membranes to finally clarify the relative importance of each cell surface component in initial cell recognition	1240:1404	These results were compared to the association of CPCs with lipid vesicles of varying composition as model plasma membranes to finally clarify the relative importance of each cell surface component in initial cell recognition.
26356251	6	25	theme	cell	1389:1392	arg1	recognition					1394:1404	initial cell recognition	1381:1404	initial cell recognition	1381:1404	These results were compared to the association of CPCs with lipid vesicles of varying composition as model plasma membranes to finally clarify the relative importance of each cell surface component in initial cell recognition.
26356251	2	26	theme	cell-penetrating	353:368	arg1	compounds					370:378	the cell-penetrating compounds	349:378	the cell-penetrating compounds	349:378	We hypothesize that the electrostatic interaction between the positively charged amines of the cell-penetrating compounds and the negatively charged glycosaminoglycans (GAGs) extending from cell surfaces is the initiating step in the internalization process.
26356251	7	27	theme	GAG	1468:1470	arg1	affinity					1472:1479	GAG affinity	1468:1479	GAG affinity	1468:1479	CPC-lipid affinity increases with anionic lipid content, but GAG affinity is higher for all cell-penetrating compounds, confirming the role these heterogeneous polysaccharides play in cellular association and clustering.
26356251	0	28	theme	Cell-penetrating	0:15	arg1	compounds					17:25	Cell-penetrating compounds	0:25	Cell-penetrating compounds	0:25	Cell-penetrating compounds preferentially bind glycosaminoglycans over plasma membrane lipids in a charge density- and stereochemistry-dependent manner.
26356251	5	29	theme	amine	1166:1170	arg1	groups					1172:1177	GAG hydroxyl and amine groups	1149:1177	groups	1172:1177	The effect of GAG composition on affinity is likely due to hydrogen bonding between CPC amines and amides and GAG hydroxyl and amine groups.
26356251	4	30	theme	molecular	1021:1029	arg1	weight					1031:1036	CPC molecular weight	1017:1036	CPC molecular weight	1017:1036	Detailed thermodynamic analysis has determined that CPC-GAG binding constants range from 8.7×10(3) to 2.4×10(6)M(-1) and that affinity is dependent upon GAG charge density and stereochemistry and CPC molecular weight.
26356251	3	31	theme	different	611:619	arg1	GAGs					621:624	four different GAGs	606:624	four different GAGs	606:624	The interactions of generation 5 PAMAM dendrimer, Tat peptide and 25 kDa linear PEI with four different GAGs have been studied using isothermal titration calorimetry to elucidate structure-function relationships that could lead to improved drug and gene delivery methods to a wide variety of cell types.
26356251	4	32	dep	8.7×10	910:915	arg1	M					932:932	2.4×10(6)M	923:932	8.7×10(3) to 2.4×10(6)M(-1)	910:936	Detailed thermodynamic analysis has determined that CPC-GAG binding constants range from 8.7×10(3) to 2.4×10(6)M(-1) and that affinity is dependent upon GAG charge density and stereochemistry and CPC molecular weight.
26356251	4	32	dep	8.7×10	910:915	arg1	to					920:921	to	920:921	to	920:921	Detailed thermodynamic analysis has determined that CPC-GAG binding constants range from 8.7×10(3) to 2.4×10(6)M(-1) and that affinity is dependent upon GAG charge density and stereochemistry and CPC molecular weight.
26356251	4	32	dep	8.7×10	910:915	arg1	-1					934:935	-1	934:935	-1	934:935	Detailed thermodynamic analysis has determined that CPC-GAG binding constants range from 8.7×10(3) to 2.4×10(6)M(-1) and that affinity is dependent upon GAG charge density and stereochemistry and CPC molecular weight.
26356251	3	33	theme	cell	809:812	arg1	types					814:818	cell types	809:818	cell types	809:818	The interactions of generation 5 PAMAM dendrimer, Tat peptide and 25 kDa linear PEI with four different GAGs have been studied using isothermal titration calorimetry to elucidate structure-function relationships that could lead to improved drug and gene delivery methods to a wide variety of cell types.
26356251	3	34	theme	PEI	597:599	arg1	interactions					521:532	The interactions	517:532	The interactions of generation 5 PAMAM dendrimer, Tat peptide and 25 kDa linear PEI with four different GAGs	517:624	The interactions of generation 5 PAMAM dendrimer, Tat peptide and 25 kDa linear PEI with four different GAGs have been studied using isothermal titration calorimetry to elucidate structure-function relationships that could lead to improved drug and gene delivery methods to a wide variety of cell types.
26356251	5	35	from	effect	1043:1048	arg1	affinity					1072:1079	affinity	1072:1079	affinity	1072:1079	The effect of GAG composition on affinity is likely due to hydrogen bonding between CPC amines and amides and GAG hydroxyl and amine groups.
26356251	2	36	theme	initiating	469:478	arg1	step					480:483	the initiating step	465:483	the initiating step in the internalization process	465:514	We hypothesize that the electrostatic interaction between the positively charged amines of the cell-penetrating compounds and the negatively charged glycosaminoglycans (GAGs) extending from cell surfaces is the initiating step in the internalization process.
26356251	2	36	theme	initiating	469:478	arg1	interaction					296:306	the electrostatic interaction	278:306	the electrostatic interaction between the positively charged amines of the cell-penetrating compounds and the negatively charged glycosaminoglycans (GAGs) extending from cell surfaces	278:460	We hypothesize that the electrostatic interaction between the positively charged amines of the cell-penetrating compounds and the negatively charged glycosaminoglycans (GAGs) extending from cell surfaces is the initiating step in the internalization process.
26356251	3	37	theme	wide	793:796	arg1	variety					798:804	a wide variety	791:804	a wide variety of cell types	791:818	The interactions of generation 5 PAMAM dendrimer, Tat peptide and 25 kDa linear PEI with four different GAGs have been studied using isothermal titration calorimetry to elucidate structure-function relationships that could lead to improved drug and gene delivery methods to a wide variety of cell types.
26356251	3	37	theme	wide	793:796	arg1	types					814:818	cell types	809:818	cell types	809:818	The interactions of generation 5 PAMAM dendrimer, Tat peptide and 25 kDa linear PEI with four different GAGs have been studied using isothermal titration calorimetry to elucidate structure-function relationships that could lead to improved drug and gene delivery methods to a wide variety of cell types.
26356251	6	38	theme	relative	1327:1334	arg1	importance					1336:1345	the relative importance	1323:1345	the relative importance of each cell surface component in initial cell recognition	1323:1404	These results were compared to the association of CPCs with lipid vesicles of varying composition as model plasma membranes to finally clarify the relative importance of each cell surface component in initial cell recognition.
26356251	3	39	theme	structure-function	696:713	arg1	relationships					715:727	structure-function relationships	696:727	structure-function relationships that could lead to improved drug and gene delivery methods to a wide variety of cell types	696:818	The interactions of generation 5 PAMAM dendrimer, Tat peptide and 25 kDa linear PEI with four different GAGs have been studied using isothermal titration calorimetry to elucidate structure-function relationships that could lead to improved drug and gene delivery methods to a wide variety of cell types.
26356251	1	40	theme	Cell-penetrating	153:168	arg1	compounds					170:178	Cell-penetrating compounds	153:178	Cell-penetrating compounds (CPCs)	153:185	Cell-penetrating compounds (CPCs) are often conjugated to drugs and genes to facilitate cellular uptake.
26356251	1	40	theme	Cell-penetrating	153:168	arg1	CPCs					181:184	CPCs	181:184	CPCs	181:184	Cell-penetrating compounds (CPCs) are often conjugated to drugs and genes to facilitate cellular uptake.
26356251	4	41	theme	binding	881:887	arg1	constants					889:897	CPC-GAG binding constants	873:897	CPC-GAG binding constants	873:897	Detailed thermodynamic analysis has determined that CPC-GAG binding constants range from 8.7×10(3) to 2.4×10(6)M(-1) and that affinity is dependent upon GAG charge density and stereochemistry and CPC molecular weight.
26356251	7	42	dep	role	1542:1545	arg1	play					1583:1586	play	1583:1586	play in cellular association and clustering	1583:1625	CPC-lipid affinity increases with anionic lipid content, but GAG affinity is higher for all cell-penetrating compounds, confirming the role these heterogeneous polysaccharides play in cellular association and clustering.
26356251	4	43	theme	CPC	1017:1019	arg1	weight					1031:1036	CPC molecular weight	1017:1036	CPC molecular weight	1017:1036	Detailed thermodynamic analysis has determined that CPC-GAG binding constants range from 8.7×10(3) to 2.4×10(6)M(-1) and that affinity is dependent upon GAG charge density and stereochemistry and CPC molecular weight.
26356251	3	44	with	interactions	521:532	arg1	GAGs					621:624	four different GAGs	606:624	four different GAGs	606:624	The interactions of generation 5 PAMAM dendrimer, Tat peptide and 25 kDa linear PEI with four different GAGs have been studied using isothermal titration calorimetry to elucidate structure-function relationships that could lead to improved drug and gene delivery methods to a wide variety of cell types.
26356251	4	45	theme	CPC-GAG	873:879	arg1	constants					889:897	CPC-GAG binding constants	873:897	CPC-GAG binding constants	873:897	Detailed thermodynamic analysis has determined that CPC-GAG binding constants range from 8.7×10(3) to 2.4×10(6)M(-1) and that affinity is dependent upon GAG charge density and stereochemistry and CPC molecular weight.
26356251	1	46	theme	cellular	241:248	arg1	uptake					250:255	cellular uptake	241:255	cellular uptake	241:255	Cell-penetrating compounds (CPCs) are often conjugated to drugs and genes to facilitate cellular uptake.
26356251	0	47	theme	membrane	78:85	arg1	lipids					87:92	plasma membrane lipids	71:92	plasma membrane lipids	71:92	Cell-penetrating compounds preferentially bind glycosaminoglycans over plasma membrane lipids in a charge density- and stereochemistry-dependent manner.
26356251	5	48	theme	GAG	1149:1151	arg1	hydroxyl					1153:1160	GAG hydroxyl and amine groups	1149:1177	hydroxyl	1153:1160	The effect of GAG composition on affinity is likely due to hydrogen bonding between CPC amines and amides and GAG hydroxyl and amine groups.
26356251	6	49	theme	initial	1381:1387	arg1	recognition					1394:1404	initial cell recognition	1381:1404	initial cell recognition	1381:1404	These results were compared to the association of CPCs with lipid vesicles of varying composition as model plasma membranes to finally clarify the relative importance of each cell surface component in initial cell recognition.
26356251	4	50	from	8.7×10	910:915	arg1	range					899:903	range	899:903	range	899:903	Detailed thermodynamic analysis has determined that CPC-GAG binding constants range from 8.7×10(3) to 2.4×10(6)M(-1) and that affinity is dependent upon GAG charge density and stereochemistry and CPC molecular weight.
26356251	7	51	theme	heterogeneous	1553:1565	arg1	polysaccharides					1567:1581	these heterogeneous polysaccharides	1547:1581	these heterogeneous polysaccharides	1547:1581	CPC-lipid affinity increases with anionic lipid content, but GAG affinity is higher for all cell-penetrating compounds, confirming the role these heterogeneous polysaccharides play in cellular association and clustering.
26356251	0	52	theme	plasma	71:76	arg1	lipids					87:92	plasma membrane lipids	71:92	plasma membrane lipids	71:92	Cell-penetrating compounds preferentially bind glycosaminoglycans over plasma membrane lipids in a charge density- and stereochemistry-dependent manner.
26356251	3	53	theme	types	814:818	arg1	variety					798:804	a wide variety	791:804	a wide variety of cell types	791:818	The interactions of generation 5 PAMAM dendrimer, Tat peptide and 25 kDa linear PEI with four different GAGs have been studied using isothermal titration calorimetry to elucidate structure-function relationships that could lead to improved drug and gene delivery methods to a wide variety of cell types.
26356251	3	53	theme	types	814:818	arg1	types					814:818	cell types	809:818	cell types	809:818	The interactions of generation 5 PAMAM dendrimer, Tat peptide and 25 kDa linear PEI with four different GAGs have been studied using isothermal titration calorimetry to elucidate structure-function relationships that could lead to improved drug and gene delivery methods to a wide variety of cell types.
26356251	5	54	theme	CPC	1123:1125	arg1	amines					1127:1132	CPC amines	1123:1132	CPC amines	1123:1132	The effect of GAG composition on affinity is likely due to hydrogen bonding between CPC amines and amides and GAG hydroxyl and amine groups.
26356251	3	55	theme	PAMAM	550:554	arg1	dendrimer					556:564	generation 5 PAMAM dendrimer	537:564	generation 5 PAMAM dendrimer	537:564	The interactions of generation 5 PAMAM dendrimer, Tat peptide and 25 kDa linear PEI with four different GAGs have been studied using isothermal titration calorimetry to elucidate structure-function relationships that could lead to improved drug and gene delivery methods to a wide variety of cell types.
26356251	7	56	theme	anionic	1441:1447	arg1	content					1455:1461	anionic lipid content	1441:1461	anionic lipid content	1441:1461	CPC-lipid affinity increases with anionic lipid content, but GAG affinity is higher for all cell-penetrating compounds, confirming the role these heterogeneous polysaccharides play in cellular association and clustering.
26356251	5	57	theme	GAG	1053:1055	arg1	composition					1057:1067	GAG composition	1053:1067	GAG composition	1053:1067	The effect of GAG composition on affinity is likely due to hydrogen bonding between CPC amines and amides and GAG hydroxyl and amine groups.
26356251	3	58	theme	titration	661:669	arg1	calorimetry					671:681	isothermal titration calorimetry	650:681	isothermal titration calorimetry	650:681	The interactions of generation 5 PAMAM dendrimer, Tat peptide and 25 kDa linear PEI with four different GAGs have been studied using isothermal titration calorimetry to elucidate structure-function relationships that could lead to improved drug and gene delivery methods to a wide variety of cell types.
26356251	6	59	with	association	1215:1225	arg1	vesicles					1246:1253	lipid vesicles	1240:1253	lipid vesicles of varying composition as model plasma membranes to finally clarify the relative importance of each cell surface component in initial cell recognition	1240:1404	These results were compared to the association of CPCs with lipid vesicles of varying composition as model plasma membranes to finally clarify the relative importance of each cell surface component in initial cell recognition.
26356251	4	60	theme	Detailed	821:828	arg1	analysis					844:851	Detailed thermodynamic analysis	821:851	Detailed thermodynamic analysis	821:851	Detailed thermodynamic analysis has determined that CPC-GAG binding constants range from 8.7×10(3) to 2.4×10(6)M(-1) and that affinity is dependent upon GAG charge density and stereochemistry and CPC molecular weight.
26356251	5	61	theme	composition	1057:1067	arg1	due					1091:1093	due	1091:1093	due	1091:1093	The effect of GAG composition on affinity is likely due to hydrogen bonding between CPC amines and amides and GAG hydroxyl and amine groups.
26356251	5	61	theme	composition	1057:1067	arg1	effect					1043:1048	The effect	1039:1048	The effect of GAG composition on affinity	1039:1079	The effect of GAG composition on affinity is likely due to hydrogen bonding between CPC amines and amides and GAG hydroxyl and amine groups.
26356251	2	62	theme	electrostatic	282:294	arg1	step					480:483	the initiating step	465:483	the initiating step in the internalization process	465:514	We hypothesize that the electrostatic interaction between the positively charged amines of the cell-penetrating compounds and the negatively charged glycosaminoglycans (GAGs) extending from cell surfaces is the initiating step in the internalization process.
26356251	2	62	theme	electrostatic	282:294	arg1	interaction					296:306	the electrostatic interaction	278:306	the electrostatic interaction between the positively charged amines of the cell-penetrating compounds and the negatively charged glycosaminoglycans (GAGs) extending from cell surfaces	278:460	We hypothesize that the electrostatic interaction between the positively charged amines of the cell-penetrating compounds and the negatively charged glycosaminoglycans (GAGs) extending from cell surfaces is the initiating step in the internalization process.
26356251	2	63	theme	compounds	370:378	arg1	glycosaminoglycans					407:424	the negatively charged glycosaminoglycans	384:424	the negatively charged glycosaminoglycans (GAGs) extending from cell surfaces	384:460	We hypothesize that the electrostatic interaction between the positively charged amines of the cell-penetrating compounds and the negatively charged glycosaminoglycans (GAGs) extending from cell surfaces is the initiating step in the internalization process.
26356251	2	63	theme	compounds	370:378	arg1	amines					339:344	the positively charged amines	316:344	the positively charged amines of the cell-penetrating compounds	316:378	We hypothesize that the electrostatic interaction between the positively charged amines of the cell-penetrating compounds and the negatively charged glycosaminoglycans (GAGs) extending from cell surfaces is the initiating step in the internalization process.
26356251	2	63	theme	compounds	370:378	arg1	GAGs					427:430	GAGs	427:430	GAGs	427:430	We hypothesize that the electrostatic interaction between the positively charged amines of the cell-penetrating compounds and the negatively charged glycosaminoglycans (GAGs) extending from cell surfaces is the initiating step in the internalization process.
26356251	4	64	theme	charge	978:983	arg1	density					985:991	GAG charge density	974:991	GAG charge density	974:991	Detailed thermodynamic analysis has determined that CPC-GAG binding constants range from 8.7×10(3) to 2.4×10(6)M(-1) and that affinity is dependent upon GAG charge density and stereochemistry and CPC molecular weight.
26356251	7	65	theme	cell-penetrating	1499:1514	arg1	compounds					1516:1524	all cell-penetrating compounds	1495:1524	all cell-penetrating compounds	1495:1524	CPC-lipid affinity increases with anionic lipid content, but GAG affinity is higher for all cell-penetrating compounds, confirming the role these heterogeneous polysaccharides play in cellular association and clustering.
26356251	3	66	theme	Tat	567:569	arg1	peptide					571:577	Tat peptide	567:577	Tat peptide	567:577	The interactions of generation 5 PAMAM dendrimer, Tat peptide and 25 kDa linear PEI with four different GAGs have been studied using isothermal titration calorimetry to elucidate structure-function relationships that could lead to improved drug and gene delivery methods to a wide variety of cell types.
26356251	7	67	theme	cellular	1591:1598	arg1	association					1600:1610	cellular association	1591:1610	cellular association	1591:1610	CPC-lipid affinity increases with anionic lipid content, but GAG affinity is higher for all cell-penetrating compounds, confirming the role these heterogeneous polysaccharides play in cellular association and clustering.
26356251	4	68	theme	GAG	974:976	arg1	density					985:991	GAG charge density	974:991	GAG charge density	974:991	Detailed thermodynamic analysis has determined that CPC-GAG binding constants range from 8.7×10(3) to 2.4×10(6)M(-1) and that affinity is dependent upon GAG charge density and stereochemistry and CPC molecular weight.
26356251	3	69	theme	kDa	586:588	arg1	PEI					597:599	25 kDa linear PEI	583:599	25 kDa linear PEI	583:599	The interactions of generation 5 PAMAM dendrimer, Tat peptide and 25 kDa linear PEI with four different GAGs have been studied using isothermal titration calorimetry to elucidate structure-function relationships that could lead to improved drug and gene delivery methods to a wide variety of cell types.
26356251	3	70	theme	isothermal	650:659	arg1	calorimetry					671:681	isothermal titration calorimetry	650:681	isothermal titration calorimetry	650:681	The interactions of generation 5 PAMAM dendrimer, Tat peptide and 25 kDa linear PEI with four different GAGs have been studied using isothermal titration calorimetry to elucidate structure-function relationships that could lead to improved drug and gene delivery methods to a wide variety of cell types.
26356251	3	71	theme	peptide	571:577	arg1	interactions					521:532	The interactions	517:532	The interactions of generation 5 PAMAM dendrimer, Tat peptide and 25 kDa linear PEI with four different GAGs	517:624	The interactions of generation 5 PAMAM dendrimer, Tat peptide and 25 kDa linear PEI with four different GAGs have been studied using isothermal titration calorimetry to elucidate structure-function relationships that could lead to improved drug and gene delivery methods to a wide variety of cell types.
26356251	6	72	theme	cell	1355:1358	arg1	component					1368:1376	each cell surface component	1350:1376	each cell surface component	1350:1376	These results were compared to the association of CPCs with lipid vesicles of varying composition as model plasma membranes to finally clarify the relative importance of each cell surface component in initial cell recognition.
25934108	1	0	theme	virus	373:377	arg1	model					341:345	a mouse model	333:345	a mouse model of foot-and-mouth disease virus (FMDV)	333:384	Water-soluble polysaccharides from Chuanminshen violaceum (CVPS) were evaluated for their physicochemical properties, monosaccharide composition, and adjuvant potential to specific cellular and humoral immune responses in a mouse model of foot-and-mouth disease virus (FMDV) vaccination.
25934108	9	1	theme	cellular	1360:1367	arg1	responses					1388:1396	both cellular and humoral immune responses	1355:1396	both cellular and humoral immune responses	1355:1396	Taken together, these results indicate that administering CVPS as an adjuvant enhances both cellular and humoral immune responses via the TLR-2 and TLR-4 signalling pathways, thereby promoting DC maturation and suppressing TGF-β expression and Treg frequency.
25934108	9	2	theme	DC	1461:1462	arg1	maturation					1464:1473	DC maturation	1461:1473	DC maturation	1461:1473	Taken together, these results indicate that administering CVPS as an adjuvant enhances both cellular and humoral immune responses via the TLR-2 and TLR-4 signalling pathways, thereby promoting DC maturation and suppressing TGF-β expression and Treg frequency.
25934108	9	3	theme	TGF-β	1491:1495	arg1	expression					1497:1506	TGF-β expression	1491:1506	TGF-β expression	1491:1506	Taken together, these results indicate that administering CVPS as an adjuvant enhances both cellular and humoral immune responses via the TLR-2 and TLR-4 signalling pathways, thereby promoting DC maturation and suppressing TGF-β expression and Treg frequency.
25934108	1	4	theme	specific	283:290	arg1	responses					320:328	specific cellular and humoral immune responses	283:328	specific cellular and humoral immune responses in a mouse model of foot-and-mouth disease virus (FMDV)	283:384	Water-soluble polysaccharides from Chuanminshen violaceum (CVPS) were evaluated for their physicochemical properties, monosaccharide composition, and adjuvant potential to specific cellular and humoral immune responses in a mouse model of foot-and-mouth disease virus (FMDV) vaccination.
25934108	0	5	theme	vaccine	94:100	arg1	responses					58:66	the immune responses	47:66	the immune responses of foot-and-mouth disease vaccine in mice	47:108	Chuanminshen violaceum polysaccharides improve the immune responses of foot-and-mouth disease vaccine in mice.
25934108	8	6	theme	Foxp3	1247:1251	arg1	cells					1261:1265	CD4(+)CD25(+)Foxp3(+) Treg cells	1234:1265	CD4(+)CD25(+)Foxp3(+) Treg cells	1234:1265	In contrast, CVPS downregulated TGF-β mRNA expression and the frequency of CD4(+)CD25(+)Foxp3(+) Treg cells.
25934108	8	7	theme	CD25	1240:1243	arg1	cells					1261:1265	CD4(+)CD25(+)Foxp3(+) Treg cells	1234:1265	CD4(+)CD25(+)Foxp3(+) Treg cells	1234:1265	In contrast, CVPS downregulated TGF-β mRNA expression and the frequency of CD4(+)CD25(+)Foxp3(+) Treg cells.
25934108	1	8	theme	cellular	292:299	arg1	responses					320:328	specific cellular and humoral immune responses	283:328	specific cellular and humoral immune responses in a mouse model of foot-and-mouth disease virus (FMDV)	283:384	Water-soluble polysaccharides from Chuanminshen violaceum (CVPS) were evaluated for their physicochemical properties, monosaccharide composition, and adjuvant potential to specific cellular and humoral immune responses in a mouse model of foot-and-mouth disease virus (FMDV) vaccination.
25934108	4	9	theme	phagocytic	718:727	arg1	capacity					729:736	the phagocytic capacity	714:736	the phagocytic capacity of peritoneal macrophages	714:762	Administering CVPS as an adjuvant significantly enhanced the phagocytic capacity of peritoneal macrophages, splenocyte proliferation, and the activity of NK cells and CTL as well as increased FMDV-specific IgG and IgG subclass antibody titers.
25934108	4	10	theme	NK	811:812	arg1	cells					814:818	NK cells	811:818	NK cells	811:818	Administering CVPS as an adjuvant significantly enhanced the phagocytic capacity of peritoneal macrophages, splenocyte proliferation, and the activity of NK cells and CTL as well as increased FMDV-specific IgG and IgG subclass antibody titers.
25934108	7	11	theme	mRNA	1146:1149	arg1	levels					1151:1156	MHC-I/II, TLR-2/4 mRNA levels	1128:1156	levels	1151:1156	Moreover, CVPS upregulated MHC-I/II, TLR-2/4 mRNA levels.
25934108	6	12	theme	CD80	1061:1064	arg1	expression					1082:1091	CD40(+), CD80(+), and CD86(+) expression	1052:1091	CD40(+), CD80(+), and CD86(+) expression on DCs	1052:1098	Additionally, CVPS enhanced CD40(+), CD80(+), and CD86(+) expression on DCs.
25934108	4	13	theme	IgG	863:865	arg1	titers					893:898	CTL as well as increased FMDV-specific IgG and IgG subclass antibody titers	824:898	CTL as well as increased FMDV-specific IgG and IgG subclass antibody titers	824:898	Administering CVPS as an adjuvant significantly enhanced the phagocytic capacity of peritoneal macrophages, splenocyte proliferation, and the activity of NK cells and CTL as well as increased FMDV-specific IgG and IgG subclass antibody titers.
25934108	3	14	theme	%	640:640	arg1	mass					584:587	a relative mass	573:587	a relative mass of 6.29%, 21.87%, 16.59%, 12.54%, 13.07%, and 28.05%, respectively	573:654	The monosaccharide components of the CVPS was rhamnose, arabinose, fucose, mannose, glucose, and galactose with a relative mass of 6.29%, 21.87%, 16.59%, 12.54%, 13.07%, and 28.05%, respectively.
25934108	9	15	theme	immune	1381:1386	arg1	responses					1388:1396	both cellular and humoral immune responses	1355:1396	both cellular and humoral immune responses	1355:1396	Taken together, these results indicate that administering CVPS as an adjuvant enhances both cellular and humoral immune responses via the TLR-2 and TLR-4 signalling pathways, thereby promoting DC maturation and suppressing TGF-β expression and Treg frequency.
25934108	9	16	theme	signalling	1422:1431	arg1	pathways					1433:1440	signalling pathways	1422:1440	signalling pathways	1422:1440	Taken together, these results indicate that administering CVPS as an adjuvant enhances both cellular and humoral immune responses via the TLR-2 and TLR-4 signalling pathways, thereby promoting DC maturation and suppressing TGF-β expression and Treg frequency.
25934108	5	17	theme	IFN-γ	988:992	arg1	expression					994:1003	IFN-γ expression	988:1003	IFN-γ expression in CD8(+) T cells	988:1021	Moreover, CVPS increased the expression of IL-2, IFN-γ, and IL-4 in CD4(+) T cells and IFN-γ expression in CD8(+) T cells.
25934108	1	18	theme	humoral	305:311	arg1	responses					320:328	specific cellular and humoral immune responses	283:328	specific cellular and humoral immune responses in a mouse model of foot-and-mouth disease virus (FMDV)	283:384	Water-soluble polysaccharides from Chuanminshen violaceum (CVPS) were evaluated for their physicochemical properties, monosaccharide composition, and adjuvant potential to specific cellular and humoral immune responses in a mouse model of foot-and-mouth disease virus (FMDV) vaccination.
25934108	9	19	theme	Treg	1512:1515	arg1	frequency					1517:1525	Treg frequency	1512:1525	Treg frequency	1512:1525	Taken together, these results indicate that administering CVPS as an adjuvant enhances both cellular and humoral immune responses via the TLR-2 and TLR-4 signalling pathways, thereby promoting DC maturation and suppressing TGF-β expression and Treg frequency.
25934108	0	20	from	responses	58:66	arg1	mice					105:108	mice	105:108	mice	105:108	Chuanminshen violaceum polysaccharides improve the immune responses of foot-and-mouth disease vaccine in mice.
25934108	2	21	theme	molecular	411:419	arg1	weight					421:426	The average molecular weight	399:426	The average molecular weight (Mw) of the CVPS	399:443	The average molecular weight (Mw) of the CVPS was 968.31 kDa.
25934108	2	21	theme	molecular	411:419	arg1	kDa					456:458	968.31 kDa	449:458	968.31 kDa	449:458	The average molecular weight (Mw) of the CVPS was 968.31 kDa.
25934108	2	21	theme	molecular	411:419	arg1	Mw					429:430	Mw	429:430	Mw	429:430	The average molecular weight (Mw) of the CVPS was 968.31 kDa.
25934108	4	22	theme	IgG	871:873	arg1	subclass					875:882	IgG subclass	871:882	IgG subclass	871:882	Administering CVPS as an adjuvant significantly enhanced the phagocytic capacity of peritoneal macrophages, splenocyte proliferation, and the activity of NK cells and CTL as well as increased FMDV-specific IgG and IgG subclass antibody titers.
25934108	8	23	theme	Treg	1256:1259	arg1	cells					1261:1265	CD4(+)CD25(+)Foxp3(+) Treg cells	1234:1265	CD4(+)CD25(+)Foxp3(+) Treg cells	1234:1265	In contrast, CVPS downregulated TGF-β mRNA expression and the frequency of CD4(+)CD25(+)Foxp3(+) Treg cells.
25934108	4	24	theme	splenocyte	765:774	arg1	proliferation					776:788	splenocyte proliferation	765:788	splenocyte proliferation	765:788	Administering CVPS as an adjuvant significantly enhanced the phagocytic capacity of peritoneal macrophages, splenocyte proliferation, and the activity of NK cells and CTL as well as increased FMDV-specific IgG and IgG subclass antibody titers.
25934108	1	25	dep	Chuanminshen	146:157	arg1	violaceum					159:167	Chuanminshen violaceum	146:167	Chuanminshen violaceum (CVPS)	146:174	Water-soluble polysaccharides from Chuanminshen violaceum (CVPS) were evaluated for their physicochemical properties, monosaccharide composition, and adjuvant potential to specific cellular and humoral immune responses in a mouse model of foot-and-mouth disease virus (FMDV) vaccination.
25934108	5	26	theme	T	976:976	arg1	cells					978:982	CD4(+) T cells	969:982	CD4(+) T cells	969:982	Moreover, CVPS increased the expression of IL-2, IFN-γ, and IL-4 in CD4(+) T cells and IFN-γ expression in CD8(+) T cells.
25934108	0	27	theme	violaceum	13:21	arg1	polysaccharides					23:37	Chuanminshen violaceum polysaccharides	0:37	Chuanminshen violaceum polysaccharides	0:37	Chuanminshen violaceum polysaccharides improve the immune responses of foot-and-mouth disease vaccine in mice.
25934108	8	28	theme	TGF-β	1191:1195	arg1	expression					1202:1211	TGF-β mRNA expression	1191:1211	TGF-β mRNA expression	1191:1211	In contrast, CVPS downregulated TGF-β mRNA expression and the frequency of CD4(+)CD25(+)Foxp3(+) Treg cells.
25934108	4	29	theme	titers	893:898	arg1	activity					799:806	the activity	795:806	the activity of NK cells and CTL as well as increased FMDV-specific IgG and IgG subclass antibody titers	795:898	Administering CVPS as an adjuvant significantly enhanced the phagocytic capacity of peritoneal macrophages, splenocyte proliferation, and the activity of NK cells and CTL as well as increased FMDV-specific IgG and IgG subclass antibody titers.
25934108	4	29	theme	titers	893:898	arg1	capacity					729:736	the phagocytic capacity	714:736	the phagocytic capacity of peritoneal macrophages	714:762	Administering CVPS as an adjuvant significantly enhanced the phagocytic capacity of peritoneal macrophages, splenocyte proliferation, and the activity of NK cells and CTL as well as increased FMDV-specific IgG and IgG subclass antibody titers.
25934108	4	29	theme	titers	893:898	arg1	proliferation					776:788	splenocyte proliferation	765:788	splenocyte proliferation	765:788	Administering CVPS as an adjuvant significantly enhanced the phagocytic capacity of peritoneal macrophages, splenocyte proliferation, and the activity of NK cells and CTL as well as increased FMDV-specific IgG and IgG subclass antibody titers.
25934108	4	30	theme	cells	814:818	arg1	activity					799:806	the activity	795:806	the activity of NK cells and CTL as well as increased FMDV-specific IgG and IgG subclass antibody titers	795:898	Administering CVPS as an adjuvant significantly enhanced the phagocytic capacity of peritoneal macrophages, splenocyte proliferation, and the activity of NK cells and CTL as well as increased FMDV-specific IgG and IgG subclass antibody titers.
25934108	4	30	theme	cells	814:818	arg1	capacity					729:736	the phagocytic capacity	714:736	the phagocytic capacity of peritoneal macrophages	714:762	Administering CVPS as an adjuvant significantly enhanced the phagocytic capacity of peritoneal macrophages, splenocyte proliferation, and the activity of NK cells and CTL as well as increased FMDV-specific IgG and IgG subclass antibody titers.
25934108	4	30	theme	cells	814:818	arg1	proliferation					776:788	splenocyte proliferation	765:788	splenocyte proliferation	765:788	Administering CVPS as an adjuvant significantly enhanced the phagocytic capacity of peritoneal macrophages, splenocyte proliferation, and the activity of NK cells and CTL as well as increased FMDV-specific IgG and IgG subclass antibody titers.
25934108	1	31	theme	physicochemical	201:215	arg1	properties					217:226	their physicochemical properties	195:226	their physicochemical properties	195:226	Water-soluble polysaccharides from Chuanminshen violaceum (CVPS) were evaluated for their physicochemical properties, monosaccharide composition, and adjuvant potential to specific cellular and humoral immune responses in a mouse model of foot-and-mouth disease virus (FMDV) vaccination.
25934108	0	32	theme	Chuanminshen	0:11	arg1	polysaccharides					23:37	Chuanminshen violaceum polysaccharides	0:37	Chuanminshen violaceum polysaccharides	0:37	Chuanminshen violaceum polysaccharides improve the immune responses of foot-and-mouth disease vaccine in mice.
25934108	1	33	theme	immune	313:318	arg1	responses					320:328	specific cellular and humoral immune responses	283:328	specific cellular and humoral immune responses in a mouse model of foot-and-mouth disease virus (FMDV)	283:384	Water-soluble polysaccharides from Chuanminshen violaceum (CVPS) were evaluated for their physicochemical properties, monosaccharide composition, and adjuvant potential to specific cellular and humoral immune responses in a mouse model of foot-and-mouth disease virus (FMDV) vaccination.
25934108	1	34	from	responses	320:328	arg1	model					341:345	a mouse model	333:345	a mouse model of foot-and-mouth disease virus (FMDV)	333:384	Water-soluble polysaccharides from Chuanminshen violaceum (CVPS) were evaluated for their physicochemical properties, monosaccharide composition, and adjuvant potential to specific cellular and humoral immune responses in a mouse model of foot-and-mouth disease virus (FMDV) vaccination.
25934108	2	35	theme	average	403:409	arg1	weight					421:426	The average molecular weight	399:426	The average molecular weight (Mw) of the CVPS	399:443	The average molecular weight (Mw) of the CVPS was 968.31 kDa.
25934108	2	35	theme	average	403:409	arg1	kDa					456:458	968.31 kDa	449:458	968.31 kDa	449:458	The average molecular weight (Mw) of the CVPS was 968.31 kDa.
25934108	2	35	theme	average	403:409	arg1	Mw					429:430	Mw	429:430	Mw	429:430	The average molecular weight (Mw) of the CVPS was 968.31 kDa.
25934108	8	36	theme	cells	1261:1265	arg1	expression					1202:1211	TGF-β mRNA expression	1191:1211	TGF-β mRNA expression	1191:1211	In contrast, CVPS downregulated TGF-β mRNA expression and the frequency of CD4(+)CD25(+)Foxp3(+) Treg cells.
25934108	8	36	theme	cells	1261:1265	arg1	frequency					1221:1229	the frequency	1217:1229	the frequency of CD4(+)CD25(+)Foxp3(+) Treg cells	1217:1265	In contrast, CVPS downregulated TGF-β mRNA expression and the frequency of CD4(+)CD25(+)Foxp3(+) Treg cells.
25934108	1	37	from	Chuanminshen	146:157	arg1	polysaccharides					125:139	Water-soluble polysaccharides	111:139	Water-soluble polysaccharides from Chuanminshen violaceum (CVPS)	111:174	Water-soluble polysaccharides from Chuanminshen violaceum (CVPS) were evaluated for their physicochemical properties, monosaccharide composition, and adjuvant potential to specific cellular and humoral immune responses in a mouse model of foot-and-mouth disease virus (FMDV) vaccination.
25934108	1	38	theme	Water-soluble	111:123	arg1	polysaccharides					125:139	Water-soluble polysaccharides	111:139	Water-soluble polysaccharides from Chuanminshen violaceum (CVPS)	111:174	Water-soluble polysaccharides from Chuanminshen violaceum (CVPS) were evaluated for their physicochemical properties, monosaccharide composition, and adjuvant potential to specific cellular and humoral immune responses in a mouse model of foot-and-mouth disease virus (FMDV) vaccination.
25934108	4	39	theme	peritoneal	741:750	arg1	macrophages					752:762	peritoneal macrophages	741:762	peritoneal macrophages	741:762	Administering CVPS as an adjuvant significantly enhanced the phagocytic capacity of peritoneal macrophages, splenocyte proliferation, and the activity of NK cells and CTL as well as increased FMDV-specific IgG and IgG subclass antibody titers.
25934108	3	40	theme	monosaccharide	465:478	arg1	rhamnose					507:514	rhamnose	507:514	rhamnose	507:514	The monosaccharide components of the CVPS was rhamnose, arabinose, fucose, mannose, glucose, and galactose with a relative mass of 6.29%, 21.87%, 16.59%, 12.54%, 13.07%, and 28.05%, respectively.
25934108	3	40	theme	monosaccharide	465:478	arg1	components					480:489	The monosaccharide components	461:489	The monosaccharide components of the CVPS	461:501	The monosaccharide components of the CVPS was rhamnose, arabinose, fucose, mannose, glucose, and galactose with a relative mass of 6.29%, 21.87%, 16.59%, 12.54%, 13.07%, and 28.05%, respectively.
25934108	2	41	theme	CVPS	440:443	arg1	weight					421:426	The average molecular weight	399:426	The average molecular weight (Mw) of the CVPS	399:443	The average molecular weight (Mw) of the CVPS was 968.31 kDa.
25934108	2	41	theme	CVPS	440:443	arg1	kDa					456:458	968.31 kDa	449:458	968.31 kDa	449:458	The average molecular weight (Mw) of the CVPS was 968.31 kDa.
25934108	2	41	theme	CVPS	440:443	arg1	Mw					429:430	Mw	429:430	Mw	429:430	The average molecular weight (Mw) of the CVPS was 968.31 kDa.
25934108	4	42	theme	Administering	657:669	arg1	CVPS					671:674	Administering CVPS	657:674	Administering CVPS as an adjuvant	657:689	Administering CVPS as an adjuvant significantly enhanced the phagocytic capacity of peritoneal macrophages, splenocyte proliferation, and the activity of NK cells and CTL as well as increased FMDV-specific IgG and IgG subclass antibody titers.
25934108	5	43	from	expression	930:939	arg1	cells					978:982	CD4(+) T cells	969:982	CD4(+) T cells	969:982	Moreover, CVPS increased the expression of IL-2, IFN-γ, and IL-4 in CD4(+) T cells and IFN-γ expression in CD8(+) T cells.
25934108	5	43	from	expression	930:939	arg1	cells					1017:1021	CD8(+) T cells	1008:1021	CD8(+) T cells	1008:1021	Moreover, CVPS increased the expression of IL-2, IFN-γ, and IL-4 in CD4(+) T cells and IFN-γ expression in CD8(+) T cells.
25934108	1	44	theme	monosaccharide	229:242	arg1	composition					244:254	monosaccharide composition	229:254	monosaccharide composition	229:254	Water-soluble polysaccharides from Chuanminshen violaceum (CVPS) were evaluated for their physicochemical properties, monosaccharide composition, and adjuvant potential to specific cellular and humoral immune responses in a mouse model of foot-and-mouth disease virus (FMDV) vaccination.
25934108	0	45	theme	immune	51:56	arg1	responses					58:66	the immune responses	47:66	the immune responses of foot-and-mouth disease vaccine in mice	47:108	Chuanminshen violaceum polysaccharides improve the immune responses of foot-and-mouth disease vaccine in mice.
25934108	4	46	theme	FMDV-specific	849:861	arg1	IgG					863:865	increased FMDV-specific IgG	839:865	increased FMDV-specific IgG	839:865	Administering CVPS as an adjuvant significantly enhanced the phagocytic capacity of peritoneal macrophages, splenocyte proliferation, and the activity of NK cells and CTL as well as increased FMDV-specific IgG and IgG subclass antibody titers.
25934108	5	47	theme	CD4	969:971	arg1	cells					978:982	CD4(+) T cells	969:982	CD4(+) T cells	969:982	Moreover, CVPS increased the expression of IL-2, IFN-γ, and IL-4 in CD4(+) T cells and IFN-γ expression in CD8(+) T cells.
25934108	3	48	theme	relative	575:582	arg1	mass					584:587	a relative mass	573:587	a relative mass of 6.29%, 21.87%, 16.59%, 12.54%, 13.07%, and 28.05%, respectively	573:654	The monosaccharide components of the CVPS was rhamnose, arabinose, fucose, mannose, glucose, and galactose with a relative mass of 6.29%, 21.87%, 16.59%, 12.54%, 13.07%, and 28.05%, respectively.
25934108	9	49	dep	TLR-2	1406:1410	arg1	pathways					1433:1440	signalling pathways	1422:1440	signalling pathways	1422:1440	Taken together, these results indicate that administering CVPS as an adjuvant enhances both cellular and humoral immune responses via the TLR-2 and TLR-4 signalling pathways, thereby promoting DC maturation and suppressing TGF-β expression and Treg frequency.
25934108	9	49	dep	TLR-2	1406:1410	arg1	the					1402:1404	the	1402:1404	the	1402:1404	Taken together, these results indicate that administering CVPS as an adjuvant enhances both cellular and humoral immune responses via the TLR-2 and TLR-4 signalling pathways, thereby promoting DC maturation and suppressing TGF-β expression and Treg frequency.
25934108	1	50	theme	mouse	335:339	arg1	model					341:345	a mouse model	333:345	a mouse model of foot-and-mouth disease virus (FMDV)	333:384	Water-soluble polysaccharides from Chuanminshen violaceum (CVPS) were evaluated for their physicochemical properties, monosaccharide composition, and adjuvant potential to specific cellular and humoral immune responses in a mouse model of foot-and-mouth disease virus (FMDV) vaccination.
25934108	4	51	theme	increased	839:847	arg1	IgG					863:865	increased FMDV-specific IgG	839:865	increased FMDV-specific IgG	839:865	Administering CVPS as an adjuvant significantly enhanced the phagocytic capacity of peritoneal macrophages, splenocyte proliferation, and the activity of NK cells and CTL as well as increased FMDV-specific IgG and IgG subclass antibody titers.
25934108	8	52	theme	CD4	1234:1236	arg1	cells					1261:1265	CD4(+)CD25(+)Foxp3(+) Treg cells	1234:1265	CD4(+)CD25(+)Foxp3(+) Treg cells	1234:1265	In contrast, CVPS downregulated TGF-β mRNA expression and the frequency of CD4(+)CD25(+)Foxp3(+) Treg cells.
25934108	5	53	theme	T	1015:1015	arg1	cells					1017:1021	CD8(+) T cells	1008:1021	CD8(+) T cells	1008:1021	Moreover, CVPS increased the expression of IL-2, IFN-γ, and IL-4 in CD4(+) T cells and IFN-γ expression in CD8(+) T cells.
25934108	3	54	with	mannose	536:542	arg1	mass					584:587	a relative mass	573:587	a relative mass of 6.29%, 21.87%, 16.59%, 12.54%, 13.07%, and 28.05%, respectively	573:654	The monosaccharide components of the CVPS was rhamnose, arabinose, fucose, mannose, glucose, and galactose with a relative mass of 6.29%, 21.87%, 16.59%, 12.54%, 13.07%, and 28.05%, respectively.
25934108	6	55	from	expression	1082:1091	arg1	DCs					1096:1098	DCs	1096:1098	DCs	1096:1098	Additionally, CVPS enhanced CD40(+), CD80(+), and CD86(+) expression on DCs.
25934108	4	56	theme	macrophages	752:762	arg1	activity					799:806	the activity	795:806	the activity of NK cells and CTL as well as increased FMDV-specific IgG and IgG subclass antibody titers	795:898	Administering CVPS as an adjuvant significantly enhanced the phagocytic capacity of peritoneal macrophages, splenocyte proliferation, and the activity of NK cells and CTL as well as increased FMDV-specific IgG and IgG subclass antibody titers.
25934108	4	56	theme	macrophages	752:762	arg1	capacity					729:736	the phagocytic capacity	714:736	the phagocytic capacity of peritoneal macrophages	714:762	Administering CVPS as an adjuvant significantly enhanced the phagocytic capacity of peritoneal macrophages, splenocyte proliferation, and the activity of NK cells and CTL as well as increased FMDV-specific IgG and IgG subclass antibody titers.
25934108	4	56	theme	macrophages	752:762	arg1	proliferation					776:788	splenocyte proliferation	765:788	splenocyte proliferation	765:788	Administering CVPS as an adjuvant significantly enhanced the phagocytic capacity of peritoneal macrophages, splenocyte proliferation, and the activity of NK cells and CTL as well as increased FMDV-specific IgG and IgG subclass antibody titers.
25934108	3	57	theme	%	604:604	arg1	mass					584:587	a relative mass	573:587	a relative mass of 6.29%, 21.87%, 16.59%, 12.54%, 13.07%, and 28.05%, respectively	573:654	The monosaccharide components of the CVPS was rhamnose, arabinose, fucose, mannose, glucose, and galactose with a relative mass of 6.29%, 21.87%, 16.59%, 12.54%, 13.07%, and 28.05%, respectively.
25934108	5	58	theme	IL-2	944:947	arg1	expression					994:1003	IFN-γ expression	988:1003	IFN-γ expression in CD8(+) T cells	988:1021	Moreover, CVPS increased the expression of IL-2, IFN-γ, and IL-4 in CD4(+) T cells and IFN-γ expression in CD8(+) T cells.
25934108	5	58	theme	IL-2	944:947	arg1	expression					930:939	the expression	926:939	the expression of IL-2, IFN-γ, and IL-4 in CD4(+) T cells	926:982	Moreover, CVPS increased the expression of IL-2, IFN-γ, and IL-4 in CD4(+) T cells and IFN-γ expression in CD8(+) T cells.
25934108	3	59	theme	CVPS	498:501	arg1	rhamnose					507:514	rhamnose	507:514	rhamnose	507:514	The monosaccharide components of the CVPS was rhamnose, arabinose, fucose, mannose, glucose, and galactose with a relative mass of 6.29%, 21.87%, 16.59%, 12.54%, 13.07%, and 28.05%, respectively.
25934108	3	59	theme	CVPS	498:501	arg1	components					480:489	The monosaccharide components	461:489	The monosaccharide components of the CVPS	461:501	The monosaccharide components of the CVPS was rhamnose, arabinose, fucose, mannose, glucose, and galactose with a relative mass of 6.29%, 21.87%, 16.59%, 12.54%, 13.07%, and 28.05%, respectively.
25934108	5	60	theme	CD8	1008:1010	arg1	cells					1017:1021	CD8(+) T cells	1008:1021	CD8(+) T cells	1008:1021	Moreover, CVPS increased the expression of IL-2, IFN-γ, and IL-4 in CD4(+) T cells and IFN-γ expression in CD8(+) T cells.
25934108	3	61	theme	%	628:628	arg1	mass					584:587	a relative mass	573:587	a relative mass of 6.29%, 21.87%, 16.59%, 12.54%, 13.07%, and 28.05%, respectively	573:654	The monosaccharide components of the CVPS was rhamnose, arabinose, fucose, mannose, glucose, and galactose with a relative mass of 6.29%, 21.87%, 16.59%, 12.54%, 13.07%, and 28.05%, respectively.
25934108	5	62	theme	IFN-γ	950:954	arg1	expression					994:1003	IFN-γ expression	988:1003	IFN-γ expression in CD8(+) T cells	988:1021	Moreover, CVPS increased the expression of IL-2, IFN-γ, and IL-4 in CD4(+) T cells and IFN-γ expression in CD8(+) T cells.
25934108	5	62	theme	IFN-γ	950:954	arg1	expression					930:939	the expression	926:939	the expression of IL-2, IFN-γ, and IL-4 in CD4(+) T cells	926:982	Moreover, CVPS increased the expression of IL-2, IFN-γ, and IL-4 in CD4(+) T cells and IFN-γ expression in CD8(+) T cells.
25934108	4	63	theme	antibody	884:891	arg1	titers					893:898	CTL as well as increased FMDV-specific IgG and IgG subclass antibody titers	824:898	CTL as well as increased FMDV-specific IgG and IgG subclass antibody titers	824:898	Administering CVPS as an adjuvant significantly enhanced the phagocytic capacity of peritoneal macrophages, splenocyte proliferation, and the activity of NK cells and CTL as well as increased FMDV-specific IgG and IgG subclass antibody titers.
25934108	6	64	theme	CD86	1074:1077	arg1	expression					1082:1091	CD40(+), CD80(+), and CD86(+) expression	1052:1091	CD40(+), CD80(+), and CD86(+) expression on DCs	1052:1098	Additionally, CVPS enhanced CD40(+), CD80(+), and CD86(+) expression on DCs.
25934108	8	65	theme	mRNA	1197:1200	arg1	expression					1202:1211	TGF-β mRNA expression	1191:1211	TGF-β mRNA expression	1191:1211	In contrast, CVPS downregulated TGF-β mRNA expression and the frequency of CD4(+)CD25(+)Foxp3(+) Treg cells.
25934108	1	66	theme	foot-and-mouth	350:363	arg1	FMDV					380:383	FMDV	380:383	FMDV	380:383	Water-soluble polysaccharides from Chuanminshen violaceum (CVPS) were evaluated for their physicochemical properties, monosaccharide composition, and adjuvant potential to specific cellular and humoral immune responses in a mouse model of foot-and-mouth disease virus (FMDV) vaccination.
25934108	1	66	theme	foot-and-mouth	350:363	arg1	virus					373:377	foot-and-mouth disease virus	350:377	foot-and-mouth disease virus (FMDV)	350:384	Water-soluble polysaccharides from Chuanminshen violaceum (CVPS) were evaluated for their physicochemical properties, monosaccharide composition, and adjuvant potential to specific cellular and humoral immune responses in a mouse model of foot-and-mouth disease virus (FMDV) vaccination.
25934108	3	67	theme	%	620:620	arg1	mass					584:587	a relative mass	573:587	a relative mass of 6.29%, 21.87%, 16.59%, 12.54%, 13.07%, and 28.05%, respectively	573:654	The monosaccharide components of the CVPS was rhamnose, arabinose, fucose, mannose, glucose, and galactose with a relative mass of 6.29%, 21.87%, 16.59%, 12.54%, 13.07%, and 28.05%, respectively.
25934108	3	68	theme	%	596:596	arg1	mass					584:587	a relative mass	573:587	a relative mass of 6.29%, 21.87%, 16.59%, 12.54%, 13.07%, and 28.05%, respectively	573:654	The monosaccharide components of the CVPS was rhamnose, arabinose, fucose, mannose, glucose, and galactose with a relative mass of 6.29%, 21.87%, 16.59%, 12.54%, 13.07%, and 28.05%, respectively.
25934108	4	69	theme	CTL	824:826	arg1	titers					893:898	CTL as well as increased FMDV-specific IgG and IgG subclass antibody titers	824:898	CTL as well as increased FMDV-specific IgG and IgG subclass antibody titers	824:898	Administering CVPS as an adjuvant significantly enhanced the phagocytic capacity of peritoneal macrophages, splenocyte proliferation, and the activity of NK cells and CTL as well as increased FMDV-specific IgG and IgG subclass antibody titers.
25934108	3	70	theme	%	612:612	arg1	mass					584:587	a relative mass	573:587	a relative mass of 6.29%, 21.87%, 16.59%, 12.54%, 13.07%, and 28.05%, respectively	573:654	The monosaccharide components of the CVPS was rhamnose, arabinose, fucose, mannose, glucose, and galactose with a relative mass of 6.29%, 21.87%, 16.59%, 12.54%, 13.07%, and 28.05%, respectively.
25934108	6	71	theme	CD40	1052:1055	arg1	expression					1082:1091	CD40(+), CD80(+), and CD86(+) expression	1052:1091	CD40(+), CD80(+), and CD86(+) expression on DCs	1052:1098	Additionally, CVPS enhanced CD40(+), CD80(+), and CD86(+) expression on DCs.
25934108	9	72	theme	humoral	1373:1379	arg1	responses					1388:1396	both cellular and humoral immune responses	1355:1396	both cellular and humoral immune responses	1355:1396	Taken together, these results indicate that administering CVPS as an adjuvant enhances both cellular and humoral immune responses via the TLR-2 and TLR-4 signalling pathways, thereby promoting DC maturation and suppressing TGF-β expression and Treg frequency.
25934108	1	73	theme	adjuvant	261:268	arg1	vaccination					386:396	adjuvant potential to specific cellular and humoral immune responses in a mouse model of foot-and-mouth disease virus (FMDV) vaccination	261:396	adjuvant potential to specific cellular and humoral immune responses in a mouse model of foot-and-mouth disease virus (FMDV) vaccination	261:396	Water-soluble polysaccharides from Chuanminshen violaceum (CVPS) were evaluated for their physicochemical properties, monosaccharide composition, and adjuvant potential to specific cellular and humoral immune responses in a mouse model of foot-and-mouth disease virus (FMDV) vaccination.
25934108	0	74	theme	disease	86:92	arg1	vaccine					94:100	foot-and-mouth disease vaccine	71:100	foot-and-mouth disease vaccine	71:100	Chuanminshen violaceum polysaccharides improve the immune responses of foot-and-mouth disease vaccine in mice.
25934108	5	75	theme	IL-4	961:964	arg1	expression					994:1003	IFN-γ expression	988:1003	IFN-γ expression in CD8(+) T cells	988:1021	Moreover, CVPS increased the expression of IL-2, IFN-γ, and IL-4 in CD4(+) T cells and IFN-γ expression in CD8(+) T cells.
25934108	5	75	theme	IL-4	961:964	arg1	expression					930:939	the expression	926:939	the expression of IL-2, IFN-γ, and IL-4 in CD4(+) T cells	926:982	Moreover, CVPS increased the expression of IL-2, IFN-γ, and IL-4 in CD4(+) T cells and IFN-γ expression in CD8(+) T cells.
25934108	9	76	dep	enhances	1346:1353	arg1	suppressing					1479:1489	suppressing	1479:1489	suppressing TGF-β expression and Treg frequency	1479:1525	Taken together, these results indicate that administering CVPS as an adjuvant enhances both cellular and humoral immune responses via the TLR-2 and TLR-4 signalling pathways, thereby promoting DC maturation and suppressing TGF-β expression and Treg frequency.
25934108	9	76	dep	enhances	1346:1353	arg1	promoting					1451:1459	promoting	1451:1459	promoting DC maturation	1451:1473	Taken together, these results indicate that administering CVPS as an adjuvant enhances both cellular and humoral immune responses via the TLR-2 and TLR-4 signalling pathways, thereby promoting DC maturation and suppressing TGF-β expression and Treg frequency.
25934108	5	77	from	expression	994:1003	arg1	cells					978:982	CD4(+) T cells	969:982	CD4(+) T cells	969:982	Moreover, CVPS increased the expression of IL-2, IFN-γ, and IL-4 in CD4(+) T cells and IFN-γ expression in CD8(+) T cells.
25934108	5	77	from	expression	994:1003	arg1	cells					1017:1021	CD8(+) T cells	1008:1021	CD8(+) T cells	1008:1021	Moreover, CVPS increased the expression of IL-2, IFN-γ, and IL-4 in CD4(+) T cells and IFN-γ expression in CD8(+) T cells.
25934108	1	78	theme	potential	270:278	arg1	vaccination					386:396	adjuvant potential to specific cellular and humoral immune responses in a mouse model of foot-and-mouth disease virus (FMDV) vaccination	261:396	adjuvant potential to specific cellular and humoral immune responses in a mouse model of foot-and-mouth disease virus (FMDV) vaccination	261:396	Water-soluble polysaccharides from Chuanminshen violaceum (CVPS) were evaluated for their physicochemical properties, monosaccharide composition, and adjuvant potential to specific cellular and humoral immune responses in a mouse model of foot-and-mouth disease virus (FMDV) vaccination.
25934108	0	79	theme	foot-and-mouth	71:84	arg1	disease					86:92	foot-and-mouth disease	71:92	foot-and-mouth disease vaccine	71:100	Chuanminshen violaceum polysaccharides improve the immune responses of foot-and-mouth disease vaccine in mice.
25934108	1	80	theme	disease	365:371	arg1	FMDV					380:383	FMDV	380:383	FMDV	380:383	Water-soluble polysaccharides from Chuanminshen violaceum (CVPS) were evaluated for their physicochemical properties, monosaccharide composition, and adjuvant potential to specific cellular and humoral immune responses in a mouse model of foot-and-mouth disease virus (FMDV) vaccination.
25934108	1	80	theme	disease	365:371	arg1	virus					373:377	foot-and-mouth disease virus	350:377	foot-and-mouth disease virus (FMDV)	350:384	Water-soluble polysaccharides from Chuanminshen violaceum (CVPS) were evaluated for their physicochemical properties, monosaccharide composition, and adjuvant potential to specific cellular and humoral immune responses in a mouse model of foot-and-mouth disease virus (FMDV) vaccination.
25934108	3	81	with	galactose	558:566	arg1	mass					584:587	a relative mass	573:587	a relative mass of 6.29%, 21.87%, 16.59%, 12.54%, 13.07%, and 28.05%, respectively	573:654	The monosaccharide components of the CVPS was rhamnose, arabinose, fucose, mannose, glucose, and galactose with a relative mass of 6.29%, 21.87%, 16.59%, 12.54%, 13.07%, and 28.05%, respectively.
25934108	3	82	with	glucose	545:551	arg1	mass					584:587	a relative mass	573:587	a relative mass of 6.29%, 21.87%, 16.59%, 12.54%, 13.07%, and 28.05%, respectively	573:654	The monosaccharide components of the CVPS was rhamnose, arabinose, fucose, mannose, glucose, and galactose with a relative mass of 6.29%, 21.87%, 16.59%, 12.54%, 13.07%, and 28.05%, respectively.
25470365	2	0	theme	cellulose	572:580	arg1	sponge					582:587	cellulose sponge	572:587	cellulose sponge	572:587	Two composite-based collection approaches were evaluated over a surface area of 3716 cm2 (four separate 929 cm2 areas), larger than the 645 cm2 prescribed by the standard Centers for Disease Control (CDC) and Prevention cellulose sponge sampling protocol for use on nonporous surfaces.
25470365	6	1	theme	Statistical	1048:1058	arg1	tests					1060:1064	Statistical tests	1048:1064	Statistical tests of the results using ANOVA	1048:1091	Statistical tests of the results using ANOVA indicate that the collection of composite samples using the modified sampling protocol is comparable to the collection of composite samples using the standard CDC protocol (p = 0.261).
25470365	10	2	theme	sampling	1980:1987	arg1	times					1989:1993	reduced sampling times	1972:1993	reduced sampling times in the field	1972:2006	The results of this study suggest that composite surface sampling, by either method presented here, could successfully be used to increase the surface area sampled per sponge sampler, resulting in reduced sampling times in the field and decreased laboratory processing cost and turn-around times.
25470365	6	3	theme	standard	1243:1250	arg1	p					1266:1266	p = 0.261	1266:1274	p = 0.261	1266:1274	Statistical tests of the results using ANOVA indicate that the collection of composite samples using the modified sampling protocol is comparable to the collection of composite samples using the standard CDC protocol (p = 0.261).
25470365	6	3	theme	standard	1243:1250	arg1	protocol					1256:1263	the standard CDC protocol	1239:1263	the standard CDC protocol (p = 0.261)	1239:1275	Statistical tests of the results using ANOVA indicate that the collection of composite samples using the modified sampling protocol is comparable to the collection of composite samples using the standard CDC protocol (p = 0.261).
25470365	4	4	theme	positive	836:843	arg1	controls					845:852	positive controls	836:852	positive controls	836:852	Differences in collection efficiency compared to positive controls and the potential for contaminant transfer for each protocol were assessed.
25470365	7	5	theme	multiple	1369:1376	arg1	passes					1378:1383	multiple passes	1369:1383	multiple passes with the sponge sampler over the same surface	1369:1429	Most of the surface-bound spores are collected on the first sampling pass, suggesting that multiple passes with the sponge sampler over the same surface may be unnecessary.
25470365	9	6	theme	Contaminant	1578:1588	arg1	transfer					1590:1597	Contaminant transfer	1578:1597	Contaminant transfer	1578:1597	Contaminant transfer occurs with both sampling protocols, but the magnitude of transfer is significantly greater when using the standard protocol than when the modified protocol is used (p<0.001).
25470365	6	7	theme	composite	1125:1133	arg1	samples					1135:1141	composite samples	1125:1141	composite samples using the modified sampling protocol	1125:1178	Statistical tests of the results using ANOVA indicate that the collection of composite samples using the modified sampling protocol is comparable to the collection of composite samples using the standard CDC protocol (p = 0.261).
25470365	6	8	theme	composite	1215:1223	arg1	samples					1225:1231	composite samples	1215:1231	composite samples using the standard CDC protocol (p = 0.261)	1215:1275	Statistical tests of the results using ANOVA indicate that the collection of composite samples using the modified sampling protocol is comparable to the collection of composite samples using the standard CDC protocol (p = 0.261).
25470365	4	9	theme	collection	802:811	arg1	efficiency					813:822	collection efficiency	802:822	collection efficiency	802:822	Differences in collection efficiency compared to positive controls and the potential for contaminant transfer for each protocol were assessed.
25470365	8	10	from	sampler	1495:1501	arg1	significant					1536:1546	significant	1536:1546	significant	1536:1546	The effect of moisture loss from the sponge sampler on collection efficiency was not significant (p = 0.720) for both methods.
25470365	8	10	from	sampler	1495:1501	arg1	effect					1455:1460	The effect	1451:1460	The effect of moisture loss from the sponge sampler on collection efficiency	1451:1526	The effect of moisture loss from the sponge sampler on collection efficiency was not significant (p = 0.720) for both methods.
25470365	8	10	from	sampler	1495:1501	arg1	loss					1474:1477	moisture loss	1465:1477	moisture loss from the sponge sampler	1465:1501	The effect of moisture loss from the sponge sampler on collection efficiency was not significant (p = 0.720) for both methods.
25470365	8	10	from	sampler	1495:1501	arg1	=					1551:1551	=	1551:1551	=	1551:1551	The effect of moisture loss from the sponge sampler on collection efficiency was not significant (p = 0.720) for both methods.
25470365	4	11	theme	contaminant	876:886	arg1	transfer					888:895	contaminant transfer	876:895	contaminant transfer	876:895	Differences in collection efficiency compared to positive controls and the potential for contaminant transfer for each protocol were assessed.
25470365	7	12	theme	first	1332:1336	arg1	pass					1347:1350	the first sampling pass	1328:1350	the first sampling pass	1328:1350	Most of the surface-bound spores are collected on the first sampling pass, suggesting that multiple passes with the sponge sampler over the same surface may be unnecessary.
25470365	10	13	theme	processing	2033:2042	arg1	cost					2044:2047	decreased laboratory processing cost and turn-around times	2012:2069	cost	2044:2047	The results of this study suggest that composite surface sampling, by either method presented here, could successfully be used to increase the surface area sampled per sponge sampler, resulting in reduced sampling times in the field and decreased laboratory processing cost and turn-around times.
25470365	1	14	theme	collection	198:207	arg1	utility					171:177	the utility	167:177	the utility of composite-based collection of surface samples for the detection of a Bacillus anthracis surrogate using cellulose sponge samplers on a nonporous stainless steel surface	167:349	A series of experiments was conducted to explore the utility of composite-based collection of surface samples for the detection of a Bacillus anthracis surrogate using cellulose sponge samplers on a nonporous stainless steel surface.
25470365	2	15	theme	standard	514:521	arg1	Centers					523:529	the standard Centers	510:529	the standard Centers for Disease Control (CDC) and Prevention cellulose sponge sampling protocol for use on nonporous surfaces	510:635	Two composite-based collection approaches were evaluated over a surface area of 3716 cm2 (four separate 929 cm2 areas), larger than the 645 cm2 prescribed by the standard Centers for Disease Control (CDC) and Prevention cellulose sponge sampling protocol for use on nonporous surfaces.
25470365	10	16	theme	decreased	2012:2020	arg1	cost					2044:2047	decreased laboratory processing cost and turn-around times	2012:2069	cost	2044:2047	The results of this study suggest that composite surface sampling, by either method presented here, could successfully be used to increase the surface area sampled per sponge sampler, resulting in reduced sampling times in the field and decreased laboratory processing cost and turn-around times.
25470365	3	17	theme	sampler	731:737	arg1	surface					709:715	only one surface	700:715	only one surface of the sponge sampler	700:737	The CDC method was also compared to a modified protocol where only one surface of the sponge sampler was used for each of the four areas composited.
25470365	1	18	theme	surface	212:218	arg1	samples					220:226	surface samples	212:226	surface samples	212:226	A series of experiments was conducted to explore the utility of composite-based collection of surface samples for the detection of a Bacillus anthracis surrogate using cellulose sponge samplers on a nonporous stainless steel surface.
25470365	4	19	from	potential	862:870	arg1	efficiency					813:822	collection efficiency	802:822	collection efficiency	802:822	Differences in collection efficiency compared to positive controls and the potential for contaminant transfer for each protocol were assessed.
25470365	7	20	theme	surface-bound	1290:1302	arg1	spores					1304:1309	the surface-bound spores	1286:1309	the surface-bound spores	1286:1309	Most of the surface-bound spores are collected on the first sampling pass, suggesting that multiple passes with the sponge sampler over the same surface may be unnecessary.
25470365	5	21	theme	additional	1000:1009	arg1	areas					1019:1023	additional surface areas	1000:1023	additional surface areas sampled	1000:1031	The impact of the loss of wetting buffer from the sponge sampler onto additional surface areas sampled was evaluated.
25470365	9	22	theme	transfer	1657:1664	arg1	greater					1683:1689	greater	1683:1689	greater	1683:1689	Contaminant transfer occurs with both sampling protocols, but the magnitude of transfer is significantly greater when using the standard protocol than when the modified protocol is used (p<0.001).
25470365	9	22	theme	transfer	1657:1664	arg1	magnitude					1644:1652	the magnitude	1640:1652	the magnitude of transfer	1640:1664	Contaminant transfer occurs with both sampling protocols, but the magnitude of transfer is significantly greater when using the standard protocol than when the modified protocol is used (p<0.001).
25470365	6	23	theme	modified	1153:1160	arg1	protocol					1171:1178	the modified sampling protocol	1149:1178	the modified sampling protocol	1149:1178	Statistical tests of the results using ANOVA indicate that the collection of composite samples using the modified sampling protocol is comparable to the collection of composite samples using the standard CDC protocol (p = 0.261).
25470365	1	24	theme	stainless	327:335	arg1	surface					343:349	a nonporous stainless steel surface	315:349	a nonporous stainless steel surface	315:349	A series of experiments was conducted to explore the utility of composite-based collection of surface samples for the detection of a Bacillus anthracis surrogate using cellulose sponge samplers on a nonporous stainless steel surface.
25470365	2	25	theme	larger	472:477	arg1	cm2					437:439	3716 cm2	432:439	3716 cm2 (four separate 929 cm2 areas)	432:469	Two composite-based collection approaches were evaluated over a surface area of 3716 cm2 (four separate 929 cm2 areas), larger than the 645 cm2 prescribed by the standard Centers for Disease Control (CDC) and Prevention cellulose sponge sampling protocol for use on nonporous surfaces.
25470365	2	25	theme	larger	472:477	arg1	areas					464:468	four separate 929 cm2 areas	442:468	four separate 929 cm2 areas	442:468	Two composite-based collection approaches were evaluated over a surface area of 3716 cm2 (four separate 929 cm2 areas), larger than the 645 cm2 prescribed by the standard Centers for Disease Control (CDC) and Prevention cellulose sponge sampling protocol for use on nonporous surfaces.
25470365	3	26	used	used	743:746	arg2	surface					709:715	only one surface	700:715	only one surface of the sponge sampler	700:737	The CDC method was also compared to a modified protocol where only one surface of the sponge sampler was used for each of the four areas composited.
25470365	0	27	theme	surface	75:81	arg1	samplers					83:90	surface samplers	75:90	surface samplers	75:90	Composite sampling of a Bacillus anthracis surrogate with cellulose sponge surface samplers from a nonporous surface.
25470365	2	28	theme	nonporous	618:626	arg1	surfaces					628:635	nonporous surfaces	618:635	nonporous surfaces	618:635	Two composite-based collection approaches were evaluated over a surface area of 3716 cm2 (four separate 929 cm2 areas), larger than the 645 cm2 prescribed by the standard Centers for Disease Control (CDC) and Prevention cellulose sponge sampling protocol for use on nonporous surfaces.
25470365	10	29	used	used	1897:1900	arg2	sampling					1832:1839	composite surface sampling	1814:1839	composite surface sampling	1814:1839	The results of this study suggest that composite surface sampling, by either method presented here, could successfully be used to increase the surface area sampled per sponge sampler, resulting in reduced sampling times in the field and decreased laboratory processing cost and turn-around times.
25470365	0	30	theme	nonporous	99:107	arg1	surface					109:115	a nonporous surface	97:115	a nonporous surface	97:115	Composite sampling of a Bacillus anthracis surrogate with cellulose sponge surface samplers from a nonporous surface.
25470365	4	31	from	Differences	787:797	arg1	efficiency					813:822	collection efficiency	802:822	collection efficiency	802:822	Differences in collection efficiency compared to positive controls and the potential for contaminant transfer for each protocol were assessed.
25470365	2	32	theme	separate	447:454	arg1	cm2					437:439	3716 cm2	432:439	3716 cm2 (four separate 929 cm2 areas)	432:469	Two composite-based collection approaches were evaluated over a surface area of 3716 cm2 (four separate 929 cm2 areas), larger than the 645 cm2 prescribed by the standard Centers for Disease Control (CDC) and Prevention cellulose sponge sampling protocol for use on nonporous surfaces.
25470365	2	32	theme	separate	447:454	arg1	areas					464:468	four separate 929 cm2 areas	442:468	four separate 929 cm2 areas	442:468	Two composite-based collection approaches were evaluated over a surface area of 3716 cm2 (four separate 929 cm2 areas), larger than the 645 cm2 prescribed by the standard Centers for Disease Control (CDC) and Prevention cellulose sponge sampling protocol for use on nonporous surfaces.
25470365	0	33	theme	Composite	0:8	arg1	sampling					10:17	Composite sampling	0:17	Composite sampling of a Bacillus anthracis surrogate with cellulose	0:66	Composite sampling of a Bacillus anthracis surrogate with cellulose sponge surface samplers from a nonporous surface.
25470365	1	34	theme	surrogate	270:278	arg1	detection					236:244	the detection	232:244	the detection of a Bacillus anthracis surrogate using cellulose sponge samplers on a nonporous stainless steel surface	232:349	A series of experiments was conducted to explore the utility of composite-based collection of surface samples for the detection of a Bacillus anthracis surrogate using cellulose sponge samplers on a nonporous stainless steel surface.
25470365	8	35	theme	loss	1474:1477	arg1	significant					1536:1546	significant	1536:1546	significant	1536:1546	The effect of moisture loss from the sponge sampler on collection efficiency was not significant (p = 0.720) for both methods.
25470365	8	35	theme	loss	1474:1477	arg1	effect					1455:1460	The effect	1451:1460	The effect of moisture loss from the sponge sampler on collection efficiency	1451:1526	The effect of moisture loss from the sponge sampler on collection efficiency was not significant (p = 0.720) for both methods.
25470365	8	35	theme	loss	1474:1477	arg1	=					1551:1551	=	1551:1551	=	1551:1551	The effect of moisture loss from the sponge sampler on collection efficiency was not significant (p = 0.720) for both methods.
25470365	10	36	from	times	1989:1993	arg1	field					2002:2006	the field	1998:2006	the field	1998:2006	The results of this study suggest that composite surface sampling, by either method presented here, could successfully be used to increase the surface area sampled per sponge sampler, resulting in reduced sampling times in the field and decreased laboratory processing cost and turn-around times.
25470365	1	37	theme	cellulose	286:294	arg1	samplers					303:310	cellulose sponge samplers	286:310	cellulose sponge samplers	286:310	A series of experiments was conducted to explore the utility of composite-based collection of surface samples for the detection of a Bacillus anthracis surrogate using cellulose sponge samplers on a nonporous stainless steel surface.
25470365	9	38	theme	modified	1738:1745	arg1	protocol					1747:1754	the modified protocol	1734:1754	the modified protocol	1734:1754	Contaminant transfer occurs with both sampling protocols, but the magnitude of transfer is significantly greater when using the standard protocol than when the modified protocol is used (p<0.001).
25470365	0	39	theme	Bacillus	24:31	arg1	surrogate					43:51	a Bacillus anthracis surrogate	22:51	a Bacillus anthracis surrogate with cellulose	22:66	Composite sampling of a Bacillus anthracis surrogate with cellulose sponge surface samplers from a nonporous surface.
25470365	2	40	theme	cm2	460:462	arg1	cm2					437:439	3716 cm2	432:439	3716 cm2 (four separate 929 cm2 areas)	432:469	Two composite-based collection approaches were evaluated over a surface area of 3716 cm2 (four separate 929 cm2 areas), larger than the 645 cm2 prescribed by the standard Centers for Disease Control (CDC) and Prevention cellulose sponge sampling protocol for use on nonporous surfaces.
25470365	2	40	theme	cm2	460:462	arg1	areas					464:468	four separate 929 cm2 areas	442:468	four separate 929 cm2 areas	442:468	Two composite-based collection approaches were evaluated over a surface area of 3716 cm2 (four separate 929 cm2 areas), larger than the 645 cm2 prescribed by the standard Centers for Disease Control (CDC) and Prevention cellulose sponge sampling protocol for use on nonporous surfaces.
25470365	7	41	with	passes	1378:1383	arg1	sampler					1401:1407	the sponge sampler	1390:1407	the sponge sampler	1390:1407	Most of the surface-bound spores are collected on the first sampling pass, suggesting that multiple passes with the sponge sampler over the same surface may be unnecessary.
25470365	5	42	theme	sponge	980:985	arg1	sampler					987:993	the sponge sampler	976:993	the sponge sampler	976:993	The impact of the loss of wetting buffer from the sponge sampler onto additional surface areas sampled was evaluated.
25470365	8	43	theme	collection	1506:1515	arg1	efficiency					1517:1526	collection efficiency	1506:1526	collection efficiency	1506:1526	The effect of moisture loss from the sponge sampler on collection efficiency was not significant (p = 0.720) for both methods.
25470365	1	44	theme	samples	220:226	arg1	collection					198:207	composite-based collection	182:207	composite-based collection of surface samples	182:226	A series of experiments was conducted to explore the utility of composite-based collection of surface samples for the detection of a Bacillus anthracis surrogate using cellulose sponge samplers on a nonporous stainless steel surface.
25470365	9	45	dep	used	1759:1762	arg1	p<0.001					1765:1771	p<0.001	1765:1771	p<0.001	1765:1771	Contaminant transfer occurs with both sampling protocols, but the magnitude of transfer is significantly greater when using the standard protocol than when the modified protocol is used (p<0.001).
25470365	10	46	from	cost	2044:2047	arg1	field					2002:2006	the field	1998:2006	the field	1998:2006	The results of this study suggest that composite surface sampling, by either method presented here, could successfully be used to increase the surface area sampled per sponge sampler, resulting in reduced sampling times in the field and decreased laboratory processing cost and turn-around times.
25470365	0	47	with	surrogate	43:51	arg1	cellulose					58:66	cellulose	58:66	cellulose	58:66	Composite sampling of a Bacillus anthracis surrogate with cellulose sponge surface samplers from a nonporous surface.
25470365	7	48	theme	same	1418:1421	arg1	surface					1423:1429	the same surface	1414:1429	the same surface	1414:1429	Most of the surface-bound spores are collected on the first sampling pass, suggesting that multiple passes with the sponge sampler over the same surface may be unnecessary.
25470365	5	49	theme	wetting	956:962	arg1	buffer					964:969	wetting buffer	956:969	wetting buffer	956:969	The impact of the loss of wetting buffer from the sponge sampler onto additional surface areas sampled was evaluated.
25470365	6	50	theme	CDC	1252:1254	arg1	p					1266:1266	p = 0.261	1266:1274	p = 0.261	1266:1274	Statistical tests of the results using ANOVA indicate that the collection of composite samples using the modified sampling protocol is comparable to the collection of composite samples using the standard CDC protocol (p = 0.261).
25470365	6	50	theme	CDC	1252:1254	arg1	protocol					1256:1263	the standard CDC protocol	1239:1263	the standard CDC protocol (p = 0.261)	1239:1275	Statistical tests of the results using ANOVA indicate that the collection of composite samples using the modified sampling protocol is comparable to the collection of composite samples using the standard CDC protocol (p = 0.261).
25470365	10	51	theme	surface	1824:1830	arg1	sampling					1832:1839	composite surface sampling	1814:1839	composite surface sampling	1814:1839	The results of this study suggest that composite surface sampling, by either method presented here, could successfully be used to increase the surface area sampled per sponge sampler, resulting in reduced sampling times in the field and decreased laboratory processing cost and turn-around times.
25470365	2	52	theme	composite-based	356:370	arg1	approaches					383:392	Two composite-based collection approaches	352:392	Two composite-based collection approaches	352:392	Two composite-based collection approaches were evaluated over a surface area of 3716 cm2 (four separate 929 cm2 areas), larger than the 645 cm2 prescribed by the standard Centers for Disease Control (CDC) and Prevention cellulose sponge sampling protocol for use on nonporous surfaces.
25470365	10	53	theme	reduced	1972:1978	arg1	times					1989:1993	reduced sampling times	1972:1993	reduced sampling times in the field	1972:2006	The results of this study suggest that composite surface sampling, by either method presented here, could successfully be used to increase the surface area sampled per sponge sampler, resulting in reduced sampling times in the field and decreased laboratory processing cost and turn-around times.
25470365	10	54	theme	study	1795:1799	arg1	results					1779:1785	The results	1775:1785	The results of this study	1775:1799	The results of this study suggest that composite surface sampling, by either method presented here, could successfully be used to increase the surface area sampled per sponge sampler, resulting in reduced sampling times in the field and decreased laboratory processing cost and turn-around times.
25470365	1	55	theme	Bacillus	251:258	arg1	surrogate					270:278	a Bacillus anthracis surrogate	249:278	a Bacillus anthracis surrogate using cellulose sponge samplers on a nonporous stainless steel surface	249:349	A series of experiments was conducted to explore the utility of composite-based collection of surface samples for the detection of a Bacillus anthracis surrogate using cellulose sponge samplers on a nonporous stainless steel surface.
25470365	7	56	theme	sponge	1394:1399	arg1	sampler					1401:1407	the sponge sampler	1390:1407	the sponge sampler	1390:1407	Most of the surface-bound spores are collected on the first sampling pass, suggesting that multiple passes with the sponge sampler over the same surface may be unnecessary.
25470365	6	57	theme	samples	1135:1141	arg1	comparable					1183:1192	comparable	1183:1192	comparable	1183:1192	Statistical tests of the results using ANOVA indicate that the collection of composite samples using the modified sampling protocol is comparable to the collection of composite samples using the standard CDC protocol (p = 0.261).
25470365	6	57	theme	samples	1135:1141	arg1	collection					1111:1120	the collection	1107:1120	the collection of composite samples using the modified sampling protocol	1107:1178	Statistical tests of the results using ANOVA indicate that the collection of composite samples using the modified sampling protocol is comparable to the collection of composite samples using the standard CDC protocol (p = 0.261).
25470365	6	58	theme	samples	1225:1231	arg1	collection					1201:1210	the collection	1197:1210	the collection of composite samples using the standard CDC protocol (p = 0.261)	1197:1275	Statistical tests of the results using ANOVA indicate that the collection of composite samples using the modified sampling protocol is comparable to the collection of composite samples using the standard CDC protocol (p = 0.261).
25470365	0	59	theme	surrogate	43:51	arg1	sampling					10:17	Composite sampling	0:17	Composite sampling of a Bacillus anthracis surrogate with cellulose	0:66	Composite sampling of a Bacillus anthracis surrogate with cellulose sponge surface samplers from a nonporous surface.
25470365	3	60	theme	modified	676:683	arg1	protocol					685:692	a modified protocol	674:692	a modified protocol where only one surface of the sponge sampler was used for each of the four areas composited	674:784	The CDC method was also compared to a modified protocol where only one surface of the sponge sampler was used for each of the four areas composited.
25470365	9	61	used	used	1759:1762	arg2	protocol					1747:1754	the modified protocol	1734:1754	the modified protocol	1734:1754	Contaminant transfer occurs with both sampling protocols, but the magnitude of transfer is significantly greater when using the standard protocol than when the modified protocol is used (p<0.001).
25470365	10	62	from	times	2065:2069	arg1	field					2002:2006	the field	1998:2006	the field	1998:2006	The results of this study suggest that composite surface sampling, by either method presented here, could successfully be used to increase the surface area sampled per sponge sampler, resulting in reduced sampling times in the field and decreased laboratory processing cost and turn-around times.
25470365	0	63	dep	Bacillus	24:31	arg1	anthracis					33:41	anthracis	33:41	anthracis	33:41	Composite sampling of a Bacillus anthracis surrogate with cellulose sponge surface samplers from a nonporous surface.
25470365	1	64	theme	composite-based	182:196	arg1	collection					198:207	composite-based collection	182:207	composite-based collection of surface samples	182:226	A series of experiments was conducted to explore the utility of composite-based collection of surface samples for the detection of a Bacillus anthracis surrogate using cellulose sponge samplers on a nonporous stainless steel surface.
25470365	9	65	theme	sampling	1616:1623	arg1	protocols					1625:1633	both sampling protocols	1611:1633	both sampling protocols	1611:1633	Contaminant transfer occurs with both sampling protocols, but the magnitude of transfer is significantly greater when using the standard protocol than when the modified protocol is used (p<0.001).
25470365	7	66	theme	sampling	1338:1345	arg1	pass					1347:1350	the first sampling pass	1328:1350	the first sampling pass	1328:1350	Most of the surface-bound spores are collected on the first sampling pass, suggesting that multiple passes with the sponge sampler over the same surface may be unnecessary.
25470365	10	67	theme	laboratory	2022:2031	arg1	cost					2044:2047	decreased laboratory processing cost and turn-around times	2012:2069	cost	2044:2047	The results of this study suggest that composite surface sampling, by either method presented here, could successfully be used to increase the surface area sampled per sponge sampler, resulting in reduced sampling times in the field and decreased laboratory processing cost and turn-around times.
25470365	6	68	theme	=	1268:1268	arg1	p					1266:1266	p = 0.261	1266:1274	p = 0.261	1266:1274	Statistical tests of the results using ANOVA indicate that the collection of composite samples using the modified sampling protocol is comparable to the collection of composite samples using the standard CDC protocol (p = 0.261).
25470365	6	68	theme	=	1268:1268	arg1	protocol					1256:1263	the standard CDC protocol	1239:1263	the standard CDC protocol (p = 0.261)	1239:1275	Statistical tests of the results using ANOVA indicate that the collection of composite samples using the modified sampling protocol is comparable to the collection of composite samples using the standard CDC protocol (p = 0.261).
25470365	1	69	dep	Bacillus	251:258	arg1	anthracis					260:268	anthracis	260:268	anthracis	260:268	A series of experiments was conducted to explore the utility of composite-based collection of surface samples for the detection of a Bacillus anthracis surrogate using cellulose sponge samplers on a nonporous stainless steel surface.
25470365	10	70	theme	turn-around	2053:2063	arg1	times					2065:2069	decreased laboratory processing cost and turn-around times	2012:2069	times	2065:2069	The results of this study suggest that composite surface sampling, by either method presented here, could successfully be used to increase the surface area sampled per sponge sampler, resulting in reduced sampling times in the field and decreased laboratory processing cost and turn-around times.
25470365	2	71	from	use	611:613	arg1	surfaces					628:635	nonporous surfaces	618:635	nonporous surfaces	618:635	Two composite-based collection approaches were evaluated over a surface area of 3716 cm2 (four separate 929 cm2 areas), larger than the 645 cm2 prescribed by the standard Centers for Disease Control (CDC) and Prevention cellulose sponge sampling protocol for use on nonporous surfaces.
25470365	3	72	theme	sponge	724:729	arg1	sampler					731:737	the sponge sampler	720:737	the sponge sampler	720:737	The CDC method was also compared to a modified protocol where only one surface of the sponge sampler was used for each of the four areas composited.
25470365	2	73	theme	Disease	535:541	arg1	Control					543:549	Disease Control	535:549	Disease Control (CDC)	535:555	Two composite-based collection approaches were evaluated over a surface area of 3716 cm2 (four separate 929 cm2 areas), larger than the 645 cm2 prescribed by the standard Centers for Disease Control (CDC) and Prevention cellulose sponge sampling protocol for use on nonporous surfaces.
25470365	2	73	theme	Disease	535:541	arg1	CDC					552:554	CDC	552:554	CDC	552:554	Two composite-based collection approaches were evaluated over a surface area of 3716 cm2 (four separate 929 cm2 areas), larger than the 645 cm2 prescribed by the standard Centers for Disease Control (CDC) and Prevention cellulose sponge sampling protocol for use on nonporous surfaces.
25470365	6	74	theme	sampling	1162:1169	arg1	protocol					1171:1178	the modified sampling protocol	1149:1178	the modified sampling protocol	1149:1178	Statistical tests of the results using ANOVA indicate that the collection of composite samples using the modified sampling protocol is comparable to the collection of composite samples using the standard CDC protocol (p = 0.261).
25470365	5	75	theme	surface	1011:1017	arg1	areas					1019:1023	additional surface areas	1000:1023	additional surface areas sampled	1000:1031	The impact of the loss of wetting buffer from the sponge sampler onto additional surface areas sampled was evaluated.
25470365	1	76	theme	experiments	130:140	arg1	series					120:125	A series	118:125	A series of experiments	118:140	A series of experiments was conducted to explore the utility of composite-based collection of surface samples for the detection of a Bacillus anthracis surrogate using cellulose sponge samplers on a nonporous stainless steel surface.
25470365	5	77	from	sampler	987:993	arg1	impact					934:939	The impact	930:939	The impact of the loss of wetting buffer from the sponge sampler onto additional surface areas sampled	930:1031	The impact of the loss of wetting buffer from the sponge sampler onto additional surface areas sampled was evaluated.
25470365	5	77	from	sampler	987:993	arg1	loss					948:951	the loss	944:951	the loss of wetting buffer from the sponge sampler	944:993	The impact of the loss of wetting buffer from the sponge sampler onto additional surface areas sampled was evaluated.
25470365	1	78	theme	steel	337:341	arg1	surface					343:349	a nonporous stainless steel surface	315:349	a nonporous stainless steel surface	315:349	A series of experiments was conducted to explore the utility of composite-based collection of surface samples for the detection of a Bacillus anthracis surrogate using cellulose sponge samplers on a nonporous stainless steel surface.
25470365	5	79	theme	loss	948:951	arg1	impact					934:939	The impact	930:939	The impact of the loss of wetting buffer from the sponge sampler onto additional surface areas sampled	930:1031	The impact of the loss of wetting buffer from the sponge sampler onto additional surface areas sampled was evaluated.
25470365	10	80	theme	surface	1918:1924	arg1	area					1926:1929	the surface area	1914:1929	the surface area sampled per sponge sampler	1914:1956	The results of this study suggest that composite surface sampling, by either method presented here, could successfully be used to increase the surface area sampled per sponge sampler, resulting in reduced sampling times in the field and decreased laboratory processing cost and turn-around times.
25470365	8	81	dep	significant	1536:1546	arg1	significant					1536:1546	significant	1536:1546	significant	1536:1546	The effect of moisture loss from the sponge sampler on collection efficiency was not significant (p = 0.720) for both methods.
25470365	8	81	dep	significant	1536:1546	arg1	=					1551:1551	=	1551:1551	=	1551:1551	The effect of moisture loss from the sponge sampler on collection efficiency was not significant (p = 0.720) for both methods.
25470365	8	81	dep	significant	1536:1546	arg1	effect					1455:1460	The effect	1451:1460	The effect of moisture loss from the sponge sampler on collection efficiency	1451:1526	The effect of moisture loss from the sponge sampler on collection efficiency was not significant (p = 0.720) for both methods.
25470365	9	82	theme	standard	1706:1713	arg1	protocol					1715:1722	the standard protocol	1702:1722	the standard protocol	1702:1722	Contaminant transfer occurs with both sampling protocols, but the magnitude of transfer is significantly greater when using the standard protocol than when the modified protocol is used (p<0.001).
25470365	8	83	theme	moisture	1465:1472	arg1	loss					1474:1477	moisture loss	1465:1477	moisture loss from the sponge sampler	1465:1501	The effect of moisture loss from the sponge sampler on collection efficiency was not significant (p = 0.720) for both methods.
25470365	2	84	theme	cm2	437:439	arg1	area					424:427	a surface area	414:427	a surface area of 3716 cm2 (four separate 929 cm2 areas), larger than the 645 cm2 prescribed by the standard Centers for Disease Control (CDC) and Prevention cellulose sponge sampling protocol for use on nonporous surfaces	414:635	Two composite-based collection approaches were evaluated over a surface area of 3716 cm2 (four separate 929 cm2 areas), larger than the 645 cm2 prescribed by the standard Centers for Disease Control (CDC) and Prevention cellulose sponge sampling protocol for use on nonporous surfaces.
25470365	8	85	theme	sponge	1488:1493	arg1	sampler					1495:1501	the sponge sampler	1484:1501	the sponge sampler	1484:1501	The effect of moisture loss from the sponge sampler on collection efficiency was not significant (p = 0.720) for both methods.
25470365	1	86	theme	sponge	296:301	arg1	samplers					303:310	cellulose sponge samplers	286:310	cellulose sponge samplers	286:310	A series of experiments was conducted to explore the utility of composite-based collection of surface samples for the detection of a Bacillus anthracis surrogate using cellulose sponge samplers on a nonporous stainless steel surface.
25470365	5	87	theme	buffer	964:969	arg1	loss					948:951	the loss	944:951	the loss of wetting buffer from the sponge sampler	944:993	The impact of the loss of wetting buffer from the sponge sampler onto additional surface areas sampled was evaluated.
25470365	2	88	theme	surface	416:422	arg1	area					424:427	a surface area	414:427	a surface area of 3716 cm2 (four separate 929 cm2 areas), larger than the 645 cm2 prescribed by the standard Centers for Disease Control (CDC) and Prevention cellulose sponge sampling protocol for use on nonporous surfaces	414:635	Two composite-based collection approaches were evaluated over a surface area of 3716 cm2 (four separate 929 cm2 areas), larger than the 645 cm2 prescribed by the standard Centers for Disease Control (CDC) and Prevention cellulose sponge sampling protocol for use on nonporous surfaces.
25470365	8	89	from	effect	1455:1460	arg1	sampler					1495:1501	the sponge sampler	1484:1501	the sponge sampler	1484:1501	The effect of moisture loss from the sponge sampler on collection efficiency was not significant (p = 0.720) for both methods.
25470365	8	89	from	effect	1455:1460	arg1	efficiency					1517:1526	collection efficiency	1506:1526	collection efficiency	1506:1526	The effect of moisture loss from the sponge sampler on collection efficiency was not significant (p = 0.720) for both methods.
25470365	2	90	theme	929	456:458	arg1	cm2					437:439	3716 cm2	432:439	3716 cm2 (four separate 929 cm2 areas)	432:469	Two composite-based collection approaches were evaluated over a surface area of 3716 cm2 (four separate 929 cm2 areas), larger than the 645 cm2 prescribed by the standard Centers for Disease Control (CDC) and Prevention cellulose sponge sampling protocol for use on nonporous surfaces.
25470365	2	90	theme	929	456:458	arg1	areas					464:468	four separate 929 cm2 areas	442:468	four separate 929 cm2 areas	442:468	Two composite-based collection approaches were evaluated over a surface area of 3716 cm2 (four separate 929 cm2 areas), larger than the 645 cm2 prescribed by the standard Centers for Disease Control (CDC) and Prevention cellulose sponge sampling protocol for use on nonporous surfaces.
25470365	3	91	theme	CDC	642:644	arg1	method					646:651	The CDC method	638:651	The CDC method	638:651	The CDC method was also compared to a modified protocol where only one surface of the sponge sampler was used for each of the four areas composited.
25470365	1	92	theme	nonporous	317:325	arg1	surface					343:349	a nonporous stainless steel surface	315:349	a nonporous stainless steel surface	315:349	A series of experiments was conducted to explore the utility of composite-based collection of surface samples for the detection of a Bacillus anthracis surrogate using cellulose sponge samplers on a nonporous stainless steel surface.
25470365	6	93	theme	results	1073:1079	arg1	tests					1060:1064	Statistical tests	1048:1064	Statistical tests of the results using ANOVA	1048:1091	Statistical tests of the results using ANOVA indicate that the collection of composite samples using the modified sampling protocol is comparable to the collection of composite samples using the standard CDC protocol (p = 0.261).
25470365	2	94	dep	Control	543:549	arg1	sponge					582:587	cellulose sponge	572:587	cellulose sponge	572:587	Two composite-based collection approaches were evaluated over a surface area of 3716 cm2 (four separate 929 cm2 areas), larger than the 645 cm2 prescribed by the standard Centers for Disease Control (CDC) and Prevention cellulose sponge sampling protocol for use on nonporous surfaces.
25470365	10	95	theme	sponge	1943:1948	arg1	sampler					1950:1956	sponge sampler	1943:1956	sponge sampler	1943:1956	The results of this study suggest that composite surface sampling, by either method presented here, could successfully be used to increase the surface area sampled per sponge sampler, resulting in reduced sampling times in the field and decreased laboratory processing cost and turn-around times.
25470365	10	96	theme	composite	1814:1822	arg1	sampling					1832:1839	composite surface sampling	1814:1839	composite surface sampling	1814:1839	The results of this study suggest that composite surface sampling, by either method presented here, could successfully be used to increase the surface area sampled per sponge sampler, resulting in reduced sampling times in the field and decreased laboratory processing cost and turn-around times.
25470365	2	97	theme	collection	372:381	arg1	approaches					383:392	Two composite-based collection approaches	352:392	Two composite-based collection approaches	352:392	Two composite-based collection approaches were evaluated over a surface area of 3716 cm2 (four separate 929 cm2 areas), larger than the 645 cm2 prescribed by the standard Centers for Disease Control (CDC) and Prevention cellulose sponge sampling protocol for use on nonporous surfaces.
25470365	5	98	from	impact	934:939	arg1	sampler					987:993	the sponge sampler	976:993	the sponge sampler	976:993	The impact of the loss of wetting buffer from the sponge sampler onto additional surface areas sampled was evaluated.
24136655	5	0	contain	containing	940:949	arg2	HA					967:968	HA	967:968	HA	967:968	The results demonstrated that a resin composition played an important role in degradation, and the retarded degradation rate was observed for the highly crosslinked resin containing hydroxyapatite (HA).
24136655	5	0	contain	containing	940:949	arg2	hydroxyapatite					951:964	hydroxyapatite	951:964	hydroxyapatite (HA)	951:969	The results demonstrated that a resin composition played an important role in degradation, and the retarded degradation rate was observed for the highly crosslinked resin containing hydroxyapatite (HA).
24136655	5	0	contain	containing	940:949	arg1	resin					934:938	the highly crosslinked resin	911:938	the highly crosslinked resin containing hydroxyapatite (HA)	911:969	The results demonstrated that a resin composition played an important role in degradation, and the retarded degradation rate was observed for the highly crosslinked resin containing hydroxyapatite (HA).
24136655	3	1	from	application	443:453	arg1	field					480:484	the biomedical field	465:484	the biomedical field	465:484	To extend application of SLA in the biomedical field, photocurable oligolactide resins were developed and examined for biodegradation and biocompatibility.
24136655	3	2	theme	oligolactide	500:511	arg1	resins					513:518	photocurable oligolactide resins	487:518	photocurable oligolactide resins	487:518	To extend application of SLA in the biomedical field, photocurable oligolactide resins were developed and examined for biodegradation and biocompatibility.
24136655	0	3	theme	potential	79:87	arg1	resin					89:93	a potential resin	77:93	Preparation and degradation study of photocurable oligolactide-HA composite: a potential resin for stereolithography application.	0:128	Preparation and degradation study of photocurable oligolactide-HA composite: a potential resin for stereolithography application.
24136655	7	4	theme	photocurable	1115:1126	arg1	resins					1141:1146	the developed photocurable oligolactide resins	1101:1146	the developed photocurable oligolactide resins	1101:1146	These findings suggest the possible use of the developed photocurable oligolactide resins in SLA manufacturing of biodegradable implants, where their degradation behaviors can be designed by varying the resin composition.
24136655	1	5	theme	technology	169:178	arg1	merging					134:140	merging	134:140	merging	134:140	The merging of stereolithography (SLA) technology to the medical field certainly benefits the manufacturing of parts, especially those patient-specific for the clinical use.
24136655	7	6	theme	developed	1105:1113	arg1	resins					1141:1146	the developed photocurable oligolactide resins	1101:1146	the developed photocurable oligolactide resins	1101:1146	These findings suggest the possible use of the developed photocurable oligolactide resins in SLA manufacturing of biodegradable implants, where their degradation behaviors can be designed by varying the resin composition.
24136655	0	7	theme	stereolithography	99:115	arg1	application					117:127	stereolithography application	99:127	stereolithography application	99:127	Preparation and degradation study of photocurable oligolactide-HA composite: a potential resin for stereolithography application.
24136655	7	8	theme	degradation	1208:1218	arg1	behaviors					1220:1228	their degradation behaviors	1202:1228	their degradation behaviors	1202:1228	These findings suggest the possible use of the developed photocurable oligolactide resins in SLA manufacturing of biodegradable implants, where their degradation behaviors can be designed by varying the resin composition.
24136655	6	9	theme	cytotoxic	981:989	arg1	sample					991:996	The less cytotoxic sample	972:996	The less cytotoxic sample	972:996	The less cytotoxic sample was also obtained from the resin with higher content of HA.
24136655	4	10	theme	specimens	715:723	arg1	changes					635:641	the changes	631:641	the changes in weight loss	631:656	The degradation was studied by monitoring the changes in weight loss, and thermal and mechanical properties of the photocured specimens in phosphate buffered saline (PBS) at 37°C.
24136655	4	10	theme	specimens	715:723	arg1	properties					686:695	thermal and mechanical properties	663:695	thermal and mechanical properties of the photocured specimens in phosphate buffered saline (PBS) at 37°C	663:766	The degradation was studied by monitoring the changes in weight loss, and thermal and mechanical properties of the photocured specimens in phosphate buffered saline (PBS) at 37°C.
24136655	4	11	from	properties	686:695	arg1	PBS					755:757	PBS	755:757	PBS	755:757	The degradation was studied by monitoring the changes in weight loss, and thermal and mechanical properties of the photocured specimens in phosphate buffered saline (PBS) at 37°C.
24136655	4	11	from	properties	686:695	arg1	loss					653:656	weight loss	646:656	weight loss	646:656	The degradation was studied by monitoring the changes in weight loss, and thermal and mechanical properties of the photocured specimens in phosphate buffered saline (PBS) at 37°C.
24136655	4	11	from	properties	686:695	arg1	saline					747:752	phosphate buffered saline	728:752	phosphate buffered saline (PBS) at 37°C	728:766	The degradation was studied by monitoring the changes in weight loss, and thermal and mechanical properties of the photocured specimens in phosphate buffered saline (PBS) at 37°C.
24136655	4	12	theme	buffered	738:745	arg1	PBS					755:757	PBS	755:757	PBS	755:757	The degradation was studied by monitoring the changes in weight loss, and thermal and mechanical properties of the photocured specimens in phosphate buffered saline (PBS) at 37°C.
24136655	4	12	theme	buffered	738:745	arg1	saline					747:752	phosphate buffered saline	728:752	phosphate buffered saline (PBS) at 37°C	728:766	The degradation was studied by monitoring the changes in weight loss, and thermal and mechanical properties of the photocured specimens in phosphate buffered saline (PBS) at 37°C.
24136655	7	13	theme	possible	1085:1092	arg1	use					1094:1096	the possible use	1081:1096	the possible use of the developed photocurable oligolactide resins in SLA manufacturing of biodegradable implants, where their degradation behaviors can be designed by varying the resin composition	1081:1277	These findings suggest the possible use of the developed photocurable oligolactide resins in SLA manufacturing of biodegradable implants, where their degradation behaviors can be designed by varying the resin composition.
24136655	4	14	theme	weight	646:651	arg1	loss					653:656	weight loss	646:656	weight loss	646:656	The degradation was studied by monitoring the changes in weight loss, and thermal and mechanical properties of the photocured specimens in phosphate buffered saline (PBS) at 37°C.
24136655	5	15	theme	crosslinked	922:932	arg1	resin					934:938	the highly crosslinked resin	911:938	the highly crosslinked resin containing hydroxyapatite (HA)	911:969	The results demonstrated that a resin composition played an important role in degradation, and the retarded degradation rate was observed for the highly crosslinked resin containing hydroxyapatite (HA).
24136655	2	16	theme	biodegradable	392:404	arg1	resins					406:411	biodegradable resins	392:411	biodegradable resins for SLA processing	392:430	This technique, however, has hardly been exploited medically due to a limited number of biodegradable resins for SLA processing.
24136655	5	17	theme	resin	801:805	arg1	composition					807:817	a resin composition	799:817	a resin composition	799:817	The results demonstrated that a resin composition played an important role in degradation, and the retarded degradation rate was observed for the highly crosslinked resin containing hydroxyapatite (HA).
24136655	7	18	theme	oligolactide	1128:1139	arg1	resins					1141:1146	the developed photocurable oligolactide resins	1101:1146	the developed photocurable oligolactide resins	1101:1146	These findings suggest the possible use of the developed photocurable oligolactide resins in SLA manufacturing of biodegradable implants, where their degradation behaviors can be designed by varying the resin composition.
24136655	7	19	theme	implants	1186:1193	arg1	manufacturing					1155:1167	SLA manufacturing	1151:1167	SLA manufacturing of biodegradable implants, where their degradation behaviors can be designed by varying the resin composition	1151:1277	These findings suggest the possible use of the developed photocurable oligolactide resins in SLA manufacturing of biodegradable implants, where their degradation behaviors can be designed by varying the resin composition.
24136655	4	20	theme	thermal	663:669	arg1	properties					686:695	thermal and mechanical properties	663:695	thermal and mechanical properties of the photocured specimens in phosphate buffered saline (PBS) at 37°C	663:766	The degradation was studied by monitoring the changes in weight loss, and thermal and mechanical properties of the photocured specimens in phosphate buffered saline (PBS) at 37°C.
24136655	7	21	theme	biodegradable	1172:1184	arg1	implants					1186:1193	biodegradable implants	1172:1193	biodegradable implants	1172:1193	These findings suggest the possible use of the developed photocurable oligolactide resins in SLA manufacturing of biodegradable implants, where their degradation behaviors can be designed by varying the resin composition.
24136655	1	22	theme	medical	187:193	arg1	field					195:199	the medical field	183:199	the medical field	183:199	The merging of stereolithography (SLA) technology to the medical field certainly benefits the manufacturing of parts, especially those patient-specific for the clinical use.
24136655	0	23	theme	Preparation	0:10	arg1	study					28:32	Preparation and degradation study	0:32	Preparation and degradation study of photocurable oligolactide-HA composite: a potential resin for stereolithography application.	0:128	Preparation and degradation study of photocurable oligolactide-HA composite: a potential resin for stereolithography application.
24136655	1	24	theme	clinical	290:297	arg1	use					299:301	the clinical use	286:301	the clinical use	286:301	The merging of stereolithography (SLA) technology to the medical field certainly benefits the manufacturing of parts, especially those patient-specific for the clinical use.
24136655	2	25	theme	limited	374:380	arg1	number					382:387	a limited number	372:387	a limited number of biodegradable resins for SLA processing	372:430	This technique, however, has hardly been exploited medically due to a limited number of biodegradable resins for SLA processing.
24136655	4	26	from	37°C	763:766	arg1	specimens					715:723	the photocured specimens	700:723	the photocured specimens in phosphate buffered saline (PBS) at 37°C	700:766	The degradation was studied by monitoring the changes in weight loss, and thermal and mechanical properties of the photocured specimens in phosphate buffered saline (PBS) at 37°C.
24136655	4	26	from	37°C	763:766	arg1	PBS					755:757	PBS	755:757	PBS	755:757	The degradation was studied by monitoring the changes in weight loss, and thermal and mechanical properties of the photocured specimens in phosphate buffered saline (PBS) at 37°C.
24136655	4	26	from	37°C	763:766	arg1	saline					747:752	phosphate buffered saline	728:752	phosphate buffered saline (PBS) at 37°C	728:766	The degradation was studied by monitoring the changes in weight loss, and thermal and mechanical properties of the photocured specimens in phosphate buffered saline (PBS) at 37°C.
24136655	0	27	theme	degradation	16:26	arg1	study					28:32	Preparation and degradation study	0:32	Preparation and degradation study of photocurable oligolactide-HA composite: a potential resin for stereolithography application.	0:128	Preparation and degradation study of photocurable oligolactide-HA composite: a potential resin for stereolithography application.
24136655	4	28	theme	photocured	704:713	arg1	specimens					715:723	the photocured specimens	700:723	the photocured specimens in phosphate buffered saline (PBS) at 37°C	700:766	The degradation was studied by monitoring the changes in weight loss, and thermal and mechanical properties of the photocured specimens in phosphate buffered saline (PBS) at 37°C.
24136655	2	29	theme	SLA	417:419	arg1	processing					421:430	SLA processing	417:430	SLA processing	417:430	This technique, however, has hardly been exploited medically due to a limited number of biodegradable resins for SLA processing.
24136655	0	30	theme	photocurable	37:48	arg1	composite					66:74	photocurable oligolactide-HA composite	37:74	photocurable oligolactide-HA composite	37:74	Preparation and degradation study of photocurable oligolactide-HA composite: a potential resin for stereolithography application.
24136655	7	31	from	use	1094:1096	arg1	manufacturing					1155:1167	SLA manufacturing	1151:1167	SLA manufacturing of biodegradable implants, where their degradation behaviors can be designed by varying the resin composition	1151:1277	These findings suggest the possible use of the developed photocurable oligolactide resins in SLA manufacturing of biodegradable implants, where their degradation behaviors can be designed by varying the resin composition.
24136655	4	32	theme	mechanical	675:684	arg1	properties					686:695	thermal and mechanical properties	663:695	thermal and mechanical properties of the photocured specimens in phosphate buffered saline (PBS) at 37°C	663:766	The degradation was studied by monitoring the changes in weight loss, and thermal and mechanical properties of the photocured specimens in phosphate buffered saline (PBS) at 37°C.
24136655	6	33	theme	higher	1036:1041	arg1	content					1043:1049	higher content	1036:1049	higher content of HA	1036:1055	The less cytotoxic sample was also obtained from the resin with higher content of HA.
24136655	7	34	theme	SLA	1151:1153	arg1	manufacturing					1155:1167	SLA manufacturing	1151:1167	SLA manufacturing of biodegradable implants, where their degradation behaviors can be designed by varying the resin composition	1151:1277	These findings suggest the possible use of the developed photocurable oligolactide resins in SLA manufacturing of biodegradable implants, where their degradation behaviors can be designed by varying the resin composition.
24136655	5	35	theme	retarded	868:875	arg1	rate					889:892	the retarded degradation rate	864:892	the retarded degradation rate	864:892	The results demonstrated that a resin composition played an important role in degradation, and the retarded degradation rate was observed for the highly crosslinked resin containing hydroxyapatite (HA).
24136655	3	36	theme	SLA	458:460	arg1	application					443:453	application	443:453	application of SLA in the biomedical field	443:484	To extend application of SLA in the biomedical field, photocurable oligolactide resins were developed and examined for biodegradation and biocompatibility.
24136655	0	37	theme	composite	66:74	arg1	study					28:32	Preparation and degradation study	0:32	Preparation and degradation study of photocurable oligolactide-HA composite: a potential resin for stereolithography application.	0:128	Preparation and degradation study of photocurable oligolactide-HA composite: a potential resin for stereolithography application.
24136655	5	38	link	crosslinked	922:932	arg1	resin					934:938	the highly crosslinked resin	911:938	the highly crosslinked resin containing hydroxyapatite (HA)	911:969	The results demonstrated that a resin composition played an important role in degradation, and the retarded degradation rate was observed for the highly crosslinked resin containing hydroxyapatite (HA).
24136655	4	39	theme	phosphate	728:736	arg1	PBS					755:757	PBS	755:757	PBS	755:757	The degradation was studied by monitoring the changes in weight loss, and thermal and mechanical properties of the photocured specimens in phosphate buffered saline (PBS) at 37°C.
24136655	4	39	theme	phosphate	728:736	arg1	saline					747:752	phosphate buffered saline	728:752	phosphate buffered saline (PBS) at 37°C	728:766	The degradation was studied by monitoring the changes in weight loss, and thermal and mechanical properties of the photocured specimens in phosphate buffered saline (PBS) at 37°C.
24136655	0	40	theme	oligolactide-HA	50:64	arg1	composite					66:74	photocurable oligolactide-HA composite	37:74	photocurable oligolactide-HA composite	37:74	Preparation and degradation study of photocurable oligolactide-HA composite: a potential resin for stereolithography application.
24136655	3	41	theme	photocurable	487:498	arg1	resins					513:518	photocurable oligolactide resins	487:518	photocurable oligolactide resins	487:518	To extend application of SLA in the biomedical field, photocurable oligolactide resins were developed and examined for biodegradation and biocompatibility.
24136655	4	42	from	saline	747:752	arg1	changes					635:641	the changes	631:641	the changes in weight loss	631:656	The degradation was studied by monitoring the changes in weight loss, and thermal and mechanical properties of the photocured specimens in phosphate buffered saline (PBS) at 37°C.
24136655	4	42	from	saline	747:752	arg1	properties					686:695	thermal and mechanical properties	663:695	thermal and mechanical properties of the photocured specimens in phosphate buffered saline (PBS) at 37°C	663:766	The degradation was studied by monitoring the changes in weight loss, and thermal and mechanical properties of the photocured specimens in phosphate buffered saline (PBS) at 37°C.
24136655	7	43	theme	resin	1261:1265	arg1	composition					1267:1277	the resin composition	1257:1277	the resin composition	1257:1277	These findings suggest the possible use of the developed photocurable oligolactide resins in SLA manufacturing of biodegradable implants, where their degradation behaviors can be designed by varying the resin composition.
24136655	7	44	theme	resins	1141:1146	arg1	use					1094:1096	the possible use	1081:1096	the possible use of the developed photocurable oligolactide resins in SLA manufacturing of biodegradable implants, where their degradation behaviors can be designed by varying the resin composition	1081:1277	These findings suggest the possible use of the developed photocurable oligolactide resins in SLA manufacturing of biodegradable implants, where their degradation behaviors can be designed by varying the resin composition.
24136655	0	45	dep	study	28:32	arg1	resin					89:93	a potential resin	77:93	Preparation and degradation study of photocurable oligolactide-HA composite: a potential resin for stereolithography application.	0:128	Preparation and degradation study of photocurable oligolactide-HA composite: a potential resin for stereolithography application.
24136655	3	46	theme	biomedical	469:478	arg1	field					480:484	the biomedical field	465:484	the biomedical field	465:484	To extend application of SLA in the biomedical field, photocurable oligolactide resins were developed and examined for biodegradation and biocompatibility.
24136655	5	47	theme	degradation	877:887	arg1	rate					889:892	the retarded degradation rate	864:892	the retarded degradation rate	864:892	The results demonstrated that a resin composition played an important role in degradation, and the retarded degradation rate was observed for the highly crosslinked resin containing hydroxyapatite (HA).
24136655	6	48	theme	HA	1054:1055	arg1	content					1043:1049	higher content	1036:1049	higher content of HA	1036:1055	The less cytotoxic sample was also obtained from the resin with higher content of HA.
24136655	1	49	theme	stereolithography	145:161	arg1	technology					169:178	stereolithography (SLA) technology	145:178	stereolithography (SLA) technology to the medical field	145:199	The merging of stereolithography (SLA) technology to the medical field certainly benefits the manufacturing of parts, especially those patient-specific for the clinical use.
24136655	1	50	theme	parts	241:245	arg1	manufacturing					224:236	the manufacturing	220:236	the manufacturing of parts, especially those patient-specific for the clinical use	220:301	The merging of stereolithography (SLA) technology to the medical field certainly benefits the manufacturing of parts, especially those patient-specific for the clinical use.
24136655	2	51	theme	resins	406:411	arg1	number					382:387	a limited number	372:387	a limited number of biodegradable resins for SLA processing	372:430	This technique, however, has hardly been exploited medically due to a limited number of biodegradable resins for SLA processing.
24136655	4	52	from	changes	635:641	arg1	PBS					755:757	PBS	755:757	PBS	755:757	The degradation was studied by monitoring the changes in weight loss, and thermal and mechanical properties of the photocured specimens in phosphate buffered saline (PBS) at 37°C.
24136655	4	52	from	changes	635:641	arg1	loss					653:656	weight loss	646:656	weight loss	646:656	The degradation was studied by monitoring the changes in weight loss, and thermal and mechanical properties of the photocured specimens in phosphate buffered saline (PBS) at 37°C.
24136655	4	52	from	changes	635:641	arg1	saline					747:752	phosphate buffered saline	728:752	phosphate buffered saline (PBS) at 37°C	728:766	The degradation was studied by monitoring the changes in weight loss, and thermal and mechanical properties of the photocured specimens in phosphate buffered saline (PBS) at 37°C.
24136655	5	53	theme	important	829:837	arg1	role					839:842	an important role	826:842	an important role	826:842	The results demonstrated that a resin composition played an important role in degradation, and the retarded degradation rate was observed for the highly crosslinked resin containing hydroxyapatite (HA).
24136655	1	54	theme	SLA	164:166	arg1	technology					169:178	stereolithography (SLA) technology	145:178	stereolithography (SLA) technology to the medical field	145:199	The merging of stereolithography (SLA) technology to the medical field certainly benefits the manufacturing of parts, especially those patient-specific for the clinical use.
24136655	4	55	from	specimens	715:723	arg1	PBS					755:757	PBS	755:757	PBS	755:757	The degradation was studied by monitoring the changes in weight loss, and thermal and mechanical properties of the photocured specimens in phosphate buffered saline (PBS) at 37°C.
24136655	4	55	from	specimens	715:723	arg1	37°C					763:766	37°C	763:766	37°C	763:766	The degradation was studied by monitoring the changes in weight loss, and thermal and mechanical properties of the photocured specimens in phosphate buffered saline (PBS) at 37°C.
24136655	4	55	from	specimens	715:723	arg1	saline					747:752	phosphate buffered saline	728:752	phosphate buffered saline (PBS) at 37°C	728:766	The degradation was studied by monitoring the changes in weight loss, and thermal and mechanical properties of the photocured specimens in phosphate buffered saline (PBS) at 37°C.
28482582	6	0	theme	matrices	1051:1058	arg1	profile					999:1005	The drug release profile	982:1005	The drug release profile of FLU-loaded uncoated and coated optimized matrices	982:1058	The drug release profile of FLU-loaded uncoated and coated optimized matrices was best fitted in Korsmeyer-Peppas model with anomalous diffusion and case-II transport driven mechanism, respectively.
28482582	3	1	theme	concentrations	535:548	arg1	Effects					465:471	Effects	465:471	Effects of polymer-blend (alginate:GG) ratios and crosslinker (CaCl2) concentrations on drug entrapment efficiency (DEE, %) and cumulative drug release after 8h (Q8h, %)	465:633	Effects of polymer-blend (alginate:GG) ratios and crosslinker (CaCl2) concentrations on drug entrapment efficiency (DEE, %) and cumulative drug release after 8h (Q8h, %) were studied to optimize the core matrices by a 32 factorial design.
28482582	1	2	theme	flurbiprofen	277:288	arg1	delivery					296:303	intragastric flurbiprofen (FLU) delivery	264:303	intragastric flurbiprofen (FLU) delivery	264:303	Novel alginate-arabic gum (AG) gel membrane coated alginate-ghatti gum (GG) modified montmorillonite (MMT) composite matrices were developed for intragastric flurbiprofen (FLU) delivery by combining floating and mucoadhesion mechanisms.
28482582	1	3	theme	FLU	291:293	arg1	delivery					296:303	intragastric flurbiprofen (FLU) delivery	264:303	intragastric flurbiprofen (FLU) delivery	264:303	Novel alginate-arabic gum (AG) gel membrane coated alginate-ghatti gum (GG) modified montmorillonite (MMT) composite matrices were developed for intragastric flurbiprofen (FLU) delivery by combining floating and mucoadhesion mechanisms.
28482582	7	4	theme	thermal	1308:1314	arg1	behaviour					1316:1324	thermal behaviour	1308:1324	thermal behaviour	1308:1324	The uncoated and coated matrices containing FLU were also characterized for drug-excipients compatibility, drug crystallinity, thermal behaviour and surface morphology.
28482582	3	5	theme	factorial	686:694	arg1	design					696:701	a 32 factorial design	681:701	a 32 factorial design	681:701	Effects of polymer-blend (alginate:GG) ratios and crosslinker (CaCl2) concentrations on drug entrapment efficiency (DEE, %) and cumulative drug release after 8h (Q8h, %) were studied to optimize the core matrices by a 32 factorial design.
28482582	1	6	theme	montmorillonite	204:218	arg1	matrices					236:243	Novel alginate-arabic gum (AG) gel membrane coated alginate-ghatti gum (GG) modified montmorillonite (MMT) composite matrices	119:243	Novel alginate-arabic gum (AG) gel membrane coated alginate-ghatti gum (GG) modified montmorillonite (MMT) composite matrices	119:243	Novel alginate-arabic gum (AG) gel membrane coated alginate-ghatti gum (GG) modified montmorillonite (MMT) composite matrices were developed for intragastric flurbiprofen (FLU) delivery by combining floating and mucoadhesion mechanisms.
28482582	6	7	theme	anomalous	1107:1115	arg1	diffusion					1117:1125	anomalous diffusion	1107:1125	anomalous diffusion	1107:1125	The drug release profile of FLU-loaded uncoated and coated optimized matrices was best fitted in Korsmeyer-Peppas model with anomalous diffusion and case-II transport driven mechanism, respectively.
28482582	5	8	dep	matrices	872:879	arg1	F-O					882:884	F-O	882:884	F-O	882:884	The alginate-AG gel membrane enveloped optimized matrices (F-O, coated) exhibited superior buoyancy, better ex vivo mucoadhesion and slower drug release rate.
28482582	1	9	theme	MMT	221:223	arg1	matrices					236:243	Novel alginate-arabic gum (AG) gel membrane coated alginate-ghatti gum (GG) modified montmorillonite (MMT) composite matrices	119:243	Novel alginate-arabic gum (AG) gel membrane coated alginate-ghatti gum (GG) modified montmorillonite (MMT) composite matrices	119:243	Novel alginate-arabic gum (AG) gel membrane coated alginate-ghatti gum (GG) modified montmorillonite (MMT) composite matrices were developed for intragastric flurbiprofen (FLU) delivery by combining floating and mucoadhesion mechanisms.
28482582	1	10	theme	composite	226:234	arg1	matrices					236:243	Novel alginate-arabic gum (AG) gel membrane coated alginate-ghatti gum (GG) modified montmorillonite (MMT) composite matrices	119:243	Novel alginate-arabic gum (AG) gel membrane coated alginate-ghatti gum (GG) modified montmorillonite (MMT) composite matrices	119:243	Novel alginate-arabic gum (AG) gel membrane coated alginate-ghatti gum (GG) modified montmorillonite (MMT) composite matrices were developed for intragastric flurbiprofen (FLU) delivery by combining floating and mucoadhesion mechanisms.
28482582	5	11	dep	ex	931:932	arg1	vivo					934:937	vivo	934:937	vivo	934:937	The alginate-AG gel membrane enveloped optimized matrices (F-O, coated) exhibited superior buoyancy, better ex vivo mucoadhesion and slower drug release rate.
28482582	3	12	theme	drug	604:607	arg1	release					609:615	cumulative drug release	593:615	cumulative drug release after 8h (Q8h, %)	593:633	Effects of polymer-blend (alginate:GG) ratios and crosslinker (CaCl2) concentrations on drug entrapment efficiency (DEE, %) and cumulative drug release after 8h (Q8h, %) were studied to optimize the core matrices by a 32 factorial design.
28482582	3	13	dep	efficiency	569:578	arg1	%					586:586	%	586:586	%	586:586	Effects of polymer-blend (alginate:GG) ratios and crosslinker (CaCl2) concentrations on drug entrapment efficiency (DEE, %) and cumulative drug release after 8h (Q8h, %) were studied to optimize the core matrices by a 32 factorial design.
28482582	3	13	dep	efficiency	569:578	arg1	DEE					581:583	DEE	581:583	DEE	581:583	Effects of polymer-blend (alginate:GG) ratios and crosslinker (CaCl2) concentrations on drug entrapment efficiency (DEE, %) and cumulative drug release after 8h (Q8h, %) were studied to optimize the core matrices by a 32 factorial design.
28482582	5	14	theme	superior	905:912	arg1	better					924:929	better	924:929	better	924:929	The alginate-AG gel membrane enveloped optimized matrices (F-O, coated) exhibited superior buoyancy, better ex vivo mucoadhesion and slower drug release rate.
28482582	5	14	theme	superior	905:912	arg1	buoyancy					914:921	superior buoyancy	905:921	superior buoyancy	905:921	The alginate-AG gel membrane enveloped optimized matrices (F-O, coated) exhibited superior buoyancy, better ex vivo mucoadhesion and slower drug release rate.
28482582	1	15	theme	membrane	154:161	arg1	matrices					236:243	Novel alginate-arabic gum (AG) gel membrane coated alginate-ghatti gum (GG) modified montmorillonite (MMT) composite matrices	119:243	Novel alginate-arabic gum (AG) gel membrane coated alginate-ghatti gum (GG) modified montmorillonite (MMT) composite matrices	119:243	Novel alginate-arabic gum (AG) gel membrane coated alginate-ghatti gum (GG) modified montmorillonite (MMT) composite matrices were developed for intragastric flurbiprofen (FLU) delivery by combining floating and mucoadhesion mechanisms.
28482582	8	16	theme	FLU	1497:1499	arg1	delivery					1485:1492	intragastric delivery	1472:1492	intragastric delivery of FLU over an extended period of time with improved therapeutic benefits	1472:1566	Thus, the newly developed alginate-AG gel membrane coated alginate-GG modified MMT composite matrices are appropriate for intragastric delivery of FLU over an extended period of time with improved therapeutic benefits.
28482582	0	17	theme	stomach-specific	79:94	arg1	delivery					109:116	stomach-specific flurbiprofen delivery	79:116	stomach-specific flurbiprofen delivery	79:116	Core-shell alginate-ghatti gum modified montmorillonite composite matrices for stomach-specific flurbiprofen delivery.
28482582	5	18	theme	enveloped	852:860	arg1	matrices					872:879	The alginate-AG gel membrane enveloped optimized matrices	823:879	The alginate-AG gel membrane enveloped optimized matrices (F-O, coated)	823:893	The alginate-AG gel membrane enveloped optimized matrices (F-O, coated) exhibited superior buoyancy, better ex vivo mucoadhesion and slower drug release rate.
28482582	8	19	theme	time	1528:1531	arg1	period					1518:1523	an extended period	1506:1523	an extended period of time with improved therapeutic benefits	1506:1566	Thus, the newly developed alginate-AG gel membrane coated alginate-GG modified MMT composite matrices are appropriate for intragastric delivery of FLU over an extended period of time with improved therapeutic benefits.
28482582	4	20	from	errors	801:806	arg1	prediction					811:820	prediction	811:820	prediction	811:820	The optimized matrices (F-O) demonstrated DEE of 91.69±1.43% and Q8h of 74.96±1.56% with minimum errors in prediction.
28482582	7	21	theme	surface	1330:1336	arg1	morphology					1338:1347	surface morphology	1330:1347	surface morphology	1330:1347	The uncoated and coated matrices containing FLU were also characterized for drug-excipients compatibility, drug crystallinity, thermal behaviour and surface morphology.
28482582	8	22	theme	improved	1538:1545	arg1	benefits					1559:1566	improved therapeutic benefits	1538:1566	improved therapeutic benefits	1538:1566	Thus, the newly developed alginate-AG gel membrane coated alginate-GG modified MMT composite matrices are appropriate for intragastric delivery of FLU over an extended period of time with improved therapeutic benefits.
28482582	0	23	theme	flurbiprofen	96:107	arg1	delivery					109:116	stomach-specific flurbiprofen delivery	79:116	stomach-specific flurbiprofen delivery	79:116	Core-shell alginate-ghatti gum modified montmorillonite composite matrices for stomach-specific flurbiprofen delivery.
28482582	0	24	theme	Core-shell	0:9	arg1	gum					27:29	Core-shell alginate-ghatti gum	0:29	Core-shell alginate-ghatti gum	0:29	Core-shell alginate-ghatti gum modified montmorillonite composite matrices for stomach-specific flurbiprofen delivery.
28482582	1	25	theme	alginate-arabic	125:139	arg1	AG					146:147	AG	146:147	AG	146:147	Novel alginate-arabic gum (AG) gel membrane coated alginate-ghatti gum (GG) modified montmorillonite (MMT) composite matrices were developed for intragastric flurbiprofen (FLU) delivery by combining floating and mucoadhesion mechanisms.
28482582	1	25	theme	alginate-arabic	125:139	arg1	gum					141:143	Novel alginate-arabic gum	119:143	Novel alginate-arabic gum (AG) gel membrane coated alginate-ghatti gum (GG) modified montmorillonite (MMT) composite matrices	119:243	Novel alginate-arabic gum (AG) gel membrane coated alginate-ghatti gum (GG) modified montmorillonite (MMT) composite matrices were developed for intragastric flurbiprofen (FLU) delivery by combining floating and mucoadhesion mechanisms.
28482582	6	26	theme	FLU-loaded	1010:1019	arg1	matrices					1051:1058	FLU-loaded uncoated and coated optimized matrices	1010:1058	FLU-loaded uncoated and coated optimized matrices	1010:1058	The drug release profile of FLU-loaded uncoated and coated optimized matrices was best fitted in Korsmeyer-Peppas model with anomalous diffusion and case-II transport driven mechanism, respectively.
28482582	8	27	theme	alginate-AG	1376:1386	arg1	appropriate					1456:1466	appropriate	1456:1466	appropriate	1456:1466	Thus, the newly developed alginate-AG gel membrane coated alginate-GG modified MMT composite matrices are appropriate for intragastric delivery of FLU over an extended period of time with improved therapeutic benefits.
28482582	8	27	theme	alginate-AG	1376:1386	arg1	matrices					1443:1450	the newly developed alginate-AG gel membrane coated alginate-GG modified MMT composite matrices	1356:1450	the newly developed alginate-AG gel membrane coated alginate-GG modified MMT composite matrices	1356:1450	Thus, the newly developed alginate-AG gel membrane coated alginate-GG modified MMT composite matrices are appropriate for intragastric delivery of FLU over an extended period of time with improved therapeutic benefits.
28482582	1	28	theme	coated	163:168	arg1	matrices					236:243	Novel alginate-arabic gum (AG) gel membrane coated alginate-ghatti gum (GG) modified montmorillonite (MMT) composite matrices	119:243	Novel alginate-arabic gum (AG) gel membrane coated alginate-ghatti gum (GG) modified montmorillonite (MMT) composite matrices	119:243	Novel alginate-arabic gum (AG) gel membrane coated alginate-ghatti gum (GG) modified montmorillonite (MMT) composite matrices were developed for intragastric flurbiprofen (FLU) delivery by combining floating and mucoadhesion mechanisms.
28482582	8	29	theme	membrane	1392:1399	arg1	appropriate					1456:1466	appropriate	1456:1466	appropriate	1456:1466	Thus, the newly developed alginate-AG gel membrane coated alginate-GG modified MMT composite matrices are appropriate for intragastric delivery of FLU over an extended period of time with improved therapeutic benefits.
28482582	8	29	theme	membrane	1392:1399	arg1	matrices					1443:1450	the newly developed alginate-AG gel membrane coated alginate-GG modified MMT composite matrices	1356:1450	the newly developed alginate-AG gel membrane coated alginate-GG modified MMT composite matrices	1356:1450	Thus, the newly developed alginate-AG gel membrane coated alginate-GG modified MMT composite matrices are appropriate for intragastric delivery of FLU over an extended period of time with improved therapeutic benefits.
28482582	0	30	theme	montmorillonite	40:54	arg1	matrices					66:73	montmorillonite composite matrices	40:73	montmorillonite composite matrices for stomach-specific flurbiprofen delivery	40:116	Core-shell alginate-ghatti gum modified montmorillonite composite matrices for stomach-specific flurbiprofen delivery.
28482582	1	31	theme	floating	318:325	arg1	mechanisms					344:353	floating and mucoadhesion mechanisms	318:353	floating and mucoadhesion mechanisms	318:353	Novel alginate-arabic gum (AG) gel membrane coated alginate-ghatti gum (GG) modified montmorillonite (MMT) composite matrices were developed for intragastric flurbiprofen (FLU) delivery by combining floating and mucoadhesion mechanisms.
28482582	5	32	theme	alginate-AG	827:837	arg1	matrices					872:879	The alginate-AG gel membrane enveloped optimized matrices	823:879	The alginate-AG gel membrane enveloped optimized matrices (F-O, coated)	823:893	The alginate-AG gel membrane enveloped optimized matrices (F-O, coated) exhibited superior buoyancy, better ex vivo mucoadhesion and slower drug release rate.
28482582	7	33	theme	drug	1288:1291	arg1	crystallinity					1293:1305	drug crystallinity	1288:1305	drug crystallinity	1288:1305	The uncoated and coated matrices containing FLU were also characterized for drug-excipients compatibility, drug crystallinity, thermal behaviour and surface morphology.
28482582	8	34	theme	alginate-GG	1408:1418	arg1	appropriate					1456:1466	appropriate	1456:1466	appropriate	1456:1466	Thus, the newly developed alginate-AG gel membrane coated alginate-GG modified MMT composite matrices are appropriate for intragastric delivery of FLU over an extended period of time with improved therapeutic benefits.
28482582	8	34	theme	alginate-GG	1408:1418	arg1	matrices					1443:1450	the newly developed alginate-AG gel membrane coated alginate-GG modified MMT composite matrices	1356:1450	the newly developed alginate-AG gel membrane coated alginate-GG modified MMT composite matrices	1356:1450	Thus, the newly developed alginate-AG gel membrane coated alginate-GG modified MMT composite matrices are appropriate for intragastric delivery of FLU over an extended period of time with improved therapeutic benefits.
28482582	2	35	theme	composite	376:384	arg1	matrices					386:393	The clay-biopolymer composite matrices	356:393	The clay-biopolymer composite matrices containing FLU as core	356:416	The clay-biopolymer composite matrices containing FLU as core were accomplished by ionic-gelation technique.
28482582	1	36	theme	mucoadhesion	331:342	arg1	mechanisms					344:353	floating and mucoadhesion mechanisms	318:353	floating and mucoadhesion mechanisms	318:353	Novel alginate-arabic gum (AG) gel membrane coated alginate-ghatti gum (GG) modified montmorillonite (MMT) composite matrices were developed for intragastric flurbiprofen (FLU) delivery by combining floating and mucoadhesion mechanisms.
28482582	6	37	theme	Korsmeyer-Peppas	1079:1094	arg1	model					1096:1100	Korsmeyer-Peppas model	1079:1100	Korsmeyer-Peppas model	1079:1100	The drug release profile of FLU-loaded uncoated and coated optimized matrices was best fitted in Korsmeyer-Peppas model with anomalous diffusion and case-II transport driven mechanism, respectively.
28482582	3	38	theme	core	664:667	arg1	matrices					669:676	the core matrices	660:676	the core matrices	660:676	Effects of polymer-blend (alginate:GG) ratios and crosslinker (CaCl2) concentrations on drug entrapment efficiency (DEE, %) and cumulative drug release after 8h (Q8h, %) were studied to optimize the core matrices by a 32 factorial design.
28482582	8	39	theme	MMT	1429:1431	arg1	appropriate					1456:1466	appropriate	1456:1466	appropriate	1456:1466	Thus, the newly developed alginate-AG gel membrane coated alginate-GG modified MMT composite matrices are appropriate for intragastric delivery of FLU over an extended period of time with improved therapeutic benefits.
28482582	8	39	theme	MMT	1429:1431	arg1	matrices					1443:1450	the newly developed alginate-AG gel membrane coated alginate-GG modified MMT composite matrices	1356:1450	the newly developed alginate-AG gel membrane coated alginate-GG modified MMT composite matrices	1356:1450	Thus, the newly developed alginate-AG gel membrane coated alginate-GG modified MMT composite matrices are appropriate for intragastric delivery of FLU over an extended period of time with improved therapeutic benefits.
28482582	1	40	theme	alginate-ghatti	170:184	arg1	matrices					236:243	Novel alginate-arabic gum (AG) gel membrane coated alginate-ghatti gum (GG) modified montmorillonite (MMT) composite matrices	119:243	Novel alginate-arabic gum (AG) gel membrane coated alginate-ghatti gum (GG) modified montmorillonite (MMT) composite matrices	119:243	Novel alginate-arabic gum (AG) gel membrane coated alginate-ghatti gum (GG) modified montmorillonite (MMT) composite matrices were developed for intragastric flurbiprofen (FLU) delivery by combining floating and mucoadhesion mechanisms.
28482582	6	41	theme	drug	986:989	arg1	profile					999:1005	The drug release profile	982:1005	The drug release profile of FLU-loaded uncoated and coated optimized matrices	982:1058	The drug release profile of FLU-loaded uncoated and coated optimized matrices was best fitted in Korsmeyer-Peppas model with anomalous diffusion and case-II transport driven mechanism, respectively.
28482582	4	42	theme	Q8h	769:771	arg1	DEE					746:748	DEE	746:748	DEE of 91.69±1.43% and Q8h of 74.96±1.56%	746:786	The optimized matrices (F-O) demonstrated DEE of 91.69±1.43% and Q8h of 74.96±1.56% with minimum errors in prediction.
28482582	5	43	theme	drug	963:966	arg1	rate					976:979	slower drug release rate	956:979	slower drug release rate	956:979	The alginate-AG gel membrane enveloped optimized matrices (F-O, coated) exhibited superior buoyancy, better ex vivo mucoadhesion and slower drug release rate.
28482582	1	44	theme	intragastric	264:275	arg1	delivery					296:303	intragastric flurbiprofen (FLU) delivery	264:303	intragastric flurbiprofen (FLU) delivery	264:303	Novel alginate-arabic gum (AG) gel membrane coated alginate-ghatti gum (GG) modified montmorillonite (MMT) composite matrices were developed for intragastric flurbiprofen (FLU) delivery by combining floating and mucoadhesion mechanisms.
28482582	6	45	theme	optimized	1041:1049	arg1	matrices					1051:1058	FLU-loaded uncoated and coated optimized matrices	1010:1058	FLU-loaded uncoated and coated optimized matrices	1010:1058	The drug release profile of FLU-loaded uncoated and coated optimized matrices was best fitted in Korsmeyer-Peppas model with anomalous diffusion and case-II transport driven mechanism, respectively.
28482582	5	46	theme	optimized	862:870	arg1	matrices					872:879	The alginate-AG gel membrane enveloped optimized matrices	823:879	The alginate-AG gel membrane enveloped optimized matrices (F-O, coated)	823:893	The alginate-AG gel membrane enveloped optimized matrices (F-O, coated) exhibited superior buoyancy, better ex vivo mucoadhesion and slower drug release rate.
28482582	3	47	theme	entrapment	558:567	arg1	efficiency					569:578	drug entrapment efficiency	553:578	drug entrapment efficiency (DEE, %)	553:587	Effects of polymer-blend (alginate:GG) ratios and crosslinker (CaCl2) concentrations on drug entrapment efficiency (DEE, %) and cumulative drug release after 8h (Q8h, %) were studied to optimize the core matrices by a 32 factorial design.
28482582	1	48	theme	modified	195:202	arg1	matrices					236:243	Novel alginate-arabic gum (AG) gel membrane coated alginate-ghatti gum (GG) modified montmorillonite (MMT) composite matrices	119:243	Novel alginate-arabic gum (AG) gel membrane coated alginate-ghatti gum (GG) modified montmorillonite (MMT) composite matrices	119:243	Novel alginate-arabic gum (AG) gel membrane coated alginate-ghatti gum (GG) modified montmorillonite (MMT) composite matrices were developed for intragastric flurbiprofen (FLU) delivery by combining floating and mucoadhesion mechanisms.
28482582	1	49	theme	GG	191:192	arg1	matrices					236:243	Novel alginate-arabic gum (AG) gel membrane coated alginate-ghatti gum (GG) modified montmorillonite (MMT) composite matrices	119:243	Novel alginate-arabic gum (AG) gel membrane coated alginate-ghatti gum (GG) modified montmorillonite (MMT) composite matrices	119:243	Novel alginate-arabic gum (AG) gel membrane coated alginate-ghatti gum (GG) modified montmorillonite (MMT) composite matrices were developed for intragastric flurbiprofen (FLU) delivery by combining floating and mucoadhesion mechanisms.
28482582	4	50	theme	minimum	793:799	arg1	errors					801:806	minimum errors	793:806	minimum errors in prediction	793:820	The optimized matrices (F-O) demonstrated DEE of 91.69±1.43% and Q8h of 74.96±1.56% with minimum errors in prediction.
28482582	6	51	theme	case-II	1131:1137	arg1	transport					1139:1147	case-II transport	1131:1147	case-II transport driven mechanism	1131:1164	The drug release profile of FLU-loaded uncoated and coated optimized matrices was best fitted in Korsmeyer-Peppas model with anomalous diffusion and case-II transport driven mechanism, respectively.
28482582	3	52	dep	release	609:615	arg1	%					632:632	%	632:632	%	632:632	Effects of polymer-blend (alginate:GG) ratios and crosslinker (CaCl2) concentrations on drug entrapment efficiency (DEE, %) and cumulative drug release after 8h (Q8h, %) were studied to optimize the core matrices by a 32 factorial design.
28482582	3	52	dep	release	609:615	arg1	Q8h					627:629	Q8h	627:629	Q8h	627:629	Effects of polymer-blend (alginate:GG) ratios and crosslinker (CaCl2) concentrations on drug entrapment efficiency (DEE, %) and cumulative drug release after 8h (Q8h, %) were studied to optimize the core matrices by a 32 factorial design.
28482582	8	53	theme	coated	1401:1406	arg1	appropriate					1456:1466	appropriate	1456:1466	appropriate	1456:1466	Thus, the newly developed alginate-AG gel membrane coated alginate-GG modified MMT composite matrices are appropriate for intragastric delivery of FLU over an extended period of time with improved therapeutic benefits.
28482582	8	53	theme	coated	1401:1406	arg1	matrices					1443:1450	the newly developed alginate-AG gel membrane coated alginate-GG modified MMT composite matrices	1356:1450	the newly developed alginate-AG gel membrane coated alginate-GG modified MMT composite matrices	1356:1450	Thus, the newly developed alginate-AG gel membrane coated alginate-GG modified MMT composite matrices are appropriate for intragastric delivery of FLU over an extended period of time with improved therapeutic benefits.
28482582	5	54	theme	gel	839:841	arg1	matrices					872:879	The alginate-AG gel membrane enveloped optimized matrices	823:879	The alginate-AG gel membrane enveloped optimized matrices (F-O, coated)	823:893	The alginate-AG gel membrane enveloped optimized matrices (F-O, coated) exhibited superior buoyancy, better ex vivo mucoadhesion and slower drug release rate.
28482582	8	55	theme	intragastric	1472:1483	arg1	delivery					1485:1492	intragastric delivery	1472:1492	intragastric delivery of FLU over an extended period of time with improved therapeutic benefits	1472:1566	Thus, the newly developed alginate-AG gel membrane coated alginate-GG modified MMT composite matrices are appropriate for intragastric delivery of FLU over an extended period of time with improved therapeutic benefits.
28482582	3	56	theme	cumulative	593:602	arg1	release					609:615	cumulative drug release	593:615	cumulative drug release after 8h (Q8h, %)	593:633	Effects of polymer-blend (alginate:GG) ratios and crosslinker (CaCl2) concentrations on drug entrapment efficiency (DEE, %) and cumulative drug release after 8h (Q8h, %) were studied to optimize the core matrices by a 32 factorial design.
28482582	4	57	theme	%	763:763	arg1	DEE					746:748	DEE	746:748	DEE of 91.69±1.43% and Q8h of 74.96±1.56%	746:786	The optimized matrices (F-O) demonstrated DEE of 91.69±1.43% and Q8h of 74.96±1.56% with minimum errors in prediction.
28482582	1	58	theme	gel	150:152	arg1	matrices					236:243	Novel alginate-arabic gum (AG) gel membrane coated alginate-ghatti gum (GG) modified montmorillonite (MMT) composite matrices	119:243	Novel alginate-arabic gum (AG) gel membrane coated alginate-ghatti gum (GG) modified montmorillonite (MMT) composite matrices	119:243	Novel alginate-arabic gum (AG) gel membrane coated alginate-ghatti gum (GG) modified montmorillonite (MMT) composite matrices were developed for intragastric flurbiprofen (FLU) delivery by combining floating and mucoadhesion mechanisms.
28482582	5	59	dep	better	924:929	arg1	rate					976:979	slower drug release rate	956:979	slower drug release rate	956:979	The alginate-AG gel membrane enveloped optimized matrices (F-O, coated) exhibited superior buoyancy, better ex vivo mucoadhesion and slower drug release rate.
28482582	5	59	dep	better	924:929	arg1	mucoadhesion					939:950	ex vivo mucoadhesion	931:950	ex vivo mucoadhesion	931:950	The alginate-AG gel membrane enveloped optimized matrices (F-O, coated) exhibited superior buoyancy, better ex vivo mucoadhesion and slower drug release rate.
28482582	7	60	theme	coated	1198:1203	arg1	matrices					1205:1212	The uncoated and coated matrices	1181:1212	The uncoated and coated matrices containing FLU	1181:1227	The uncoated and coated matrices containing FLU were also characterized for drug-excipients compatibility, drug crystallinity, thermal behaviour and surface morphology.
28482582	5	61	theme	membrane	843:850	arg1	matrices					872:879	The alginate-AG gel membrane enveloped optimized matrices	823:879	The alginate-AG gel membrane enveloped optimized matrices (F-O, coated)	823:893	The alginate-AG gel membrane enveloped optimized matrices (F-O, coated) exhibited superior buoyancy, better ex vivo mucoadhesion and slower drug release rate.
28482582	8	62	theme	extended	1509:1516	arg1	period					1518:1523	an extended period	1506:1523	an extended period of time with improved therapeutic benefits	1506:1566	Thus, the newly developed alginate-AG gel membrane coated alginate-GG modified MMT composite matrices are appropriate for intragastric delivery of FLU over an extended period of time with improved therapeutic benefits.
28482582	7	63	theme	uncoated	1185:1192	arg1	matrices					1205:1212	The uncoated and coated matrices	1181:1212	The uncoated and coated matrices containing FLU	1181:1227	The uncoated and coated matrices containing FLU were also characterized for drug-excipients compatibility, drug crystallinity, thermal behaviour and surface morphology.
28482582	7	64	contain	containing	1214:1223	arg2	FLU					1225:1227	FLU	1225:1227	FLU	1225:1227	The uncoated and coated matrices containing FLU were also characterized for drug-excipients compatibility, drug crystallinity, thermal behaviour and surface morphology.
28482582	7	64	contain	containing	1214:1223	arg1	matrices					1205:1212	The uncoated and coated matrices	1181:1212	The uncoated and coated matrices containing FLU	1181:1227	The uncoated and coated matrices containing FLU were also characterized for drug-excipients compatibility, drug crystallinity, thermal behaviour and surface morphology.
28482582	1	65	theme	gum	186:188	arg1	matrices					236:243	Novel alginate-arabic gum (AG) gel membrane coated alginate-ghatti gum (GG) modified montmorillonite (MMT) composite matrices	119:243	Novel alginate-arabic gum (AG) gel membrane coated alginate-ghatti gum (GG) modified montmorillonite (MMT) composite matrices	119:243	Novel alginate-arabic gum (AG) gel membrane coated alginate-ghatti gum (GG) modified montmorillonite (MMT) composite matrices were developed for intragastric flurbiprofen (FLU) delivery by combining floating and mucoadhesion mechanisms.
28482582	3	66	from	Effects	465:471	arg1	release					609:615	cumulative drug release	593:615	cumulative drug release after 8h (Q8h, %)	593:633	Effects of polymer-blend (alginate:GG) ratios and crosslinker (CaCl2) concentrations on drug entrapment efficiency (DEE, %) and cumulative drug release after 8h (Q8h, %) were studied to optimize the core matrices by a 32 factorial design.
28482582	3	66	from	Effects	465:471	arg1	efficiency					569:578	drug entrapment efficiency	553:578	drug entrapment efficiency (DEE, %)	553:587	Effects of polymer-blend (alginate:GG) ratios and crosslinker (CaCl2) concentrations on drug entrapment efficiency (DEE, %) and cumulative drug release after 8h (Q8h, %) were studied to optimize the core matrices by a 32 factorial design.
28482582	2	67	contain	containing	395:404	arg2	FLU					406:408	FLU	406:408	FLU	406:408	The clay-biopolymer composite matrices containing FLU as core were accomplished by ionic-gelation technique.
28482582	2	67	contain	containing	395:404	arg1	matrices					386:393	The clay-biopolymer composite matrices	356:393	The clay-biopolymer composite matrices containing FLU as core	356:416	The clay-biopolymer composite matrices containing FLU as core were accomplished by ionic-gelation technique.
28482582	5	68	theme	ex	931:932	arg1	mucoadhesion					939:950	ex vivo mucoadhesion	931:950	ex vivo mucoadhesion	931:950	The alginate-AG gel membrane enveloped optimized matrices (F-O, coated) exhibited superior buoyancy, better ex vivo mucoadhesion and slower drug release rate.
28482582	6	69	theme	coated	1034:1039	arg1	matrices					1051:1058	FLU-loaded uncoated and coated optimized matrices	1010:1058	FLU-loaded uncoated and coated optimized matrices	1010:1058	The drug release profile of FLU-loaded uncoated and coated optimized matrices was best fitted in Korsmeyer-Peppas model with anomalous diffusion and case-II transport driven mechanism, respectively.
28482582	8	70	theme	therapeutic	1547:1557	arg1	benefits					1559:1566	improved therapeutic benefits	1538:1566	improved therapeutic benefits	1538:1566	Thus, the newly developed alginate-AG gel membrane coated alginate-GG modified MMT composite matrices are appropriate for intragastric delivery of FLU over an extended period of time with improved therapeutic benefits.
28482582	5	71	dep	F-O	882:884	arg1	coated					887:892	coated	887:892	coated	887:892	The alginate-AG gel membrane enveloped optimized matrices (F-O, coated) exhibited superior buoyancy, better ex vivo mucoadhesion and slower drug release rate.
28482582	3	72	theme	alginate	491:498	arg1	GG					500:501	alginate:GG	491:501	alginate:GG	491:501	Effects of polymer-blend (alginate:GG) ratios and crosslinker (CaCl2) concentrations on drug entrapment efficiency (DEE, %) and cumulative drug release after 8h (Q8h, %) were studied to optimize the core matrices by a 32 factorial design.
28482582	3	72	theme	alginate	491:498	arg1	polymer-blend					476:488	polymer-blend	476:488	polymer-blend (alginate:GG) ratios	476:509	Effects of polymer-blend (alginate:GG) ratios and crosslinker (CaCl2) concentrations on drug entrapment efficiency (DEE, %) and cumulative drug release after 8h (Q8h, %) were studied to optimize the core matrices by a 32 factorial design.
28482582	0	73	theme	alginate-ghatti	11:25	arg1	gum					27:29	Core-shell alginate-ghatti gum	0:29	Core-shell alginate-ghatti gum	0:29	Core-shell alginate-ghatti gum modified montmorillonite composite matrices for stomach-specific flurbiprofen delivery.
28482582	1	74	theme	Novel	119:123	arg1	AG					146:147	AG	146:147	AG	146:147	Novel alginate-arabic gum (AG) gel membrane coated alginate-ghatti gum (GG) modified montmorillonite (MMT) composite matrices were developed for intragastric flurbiprofen (FLU) delivery by combining floating and mucoadhesion mechanisms.
28482582	1	74	theme	Novel	119:123	arg1	gum					141:143	Novel alginate-arabic gum	119:143	Novel alginate-arabic gum (AG) gel membrane coated alginate-ghatti gum (GG) modified montmorillonite (MMT) composite matrices	119:243	Novel alginate-arabic gum (AG) gel membrane coated alginate-ghatti gum (GG) modified montmorillonite (MMT) composite matrices were developed for intragastric flurbiprofen (FLU) delivery by combining floating and mucoadhesion mechanisms.
28482582	6	75	theme	uncoated	1021:1028	arg1	matrices					1051:1058	FLU-loaded uncoated and coated optimized matrices	1010:1058	FLU-loaded uncoated and coated optimized matrices	1010:1058	The drug release profile of FLU-loaded uncoated and coated optimized matrices was best fitted in Korsmeyer-Peppas model with anomalous diffusion and case-II transport driven mechanism, respectively.
28482582	4	76	theme	optimized	708:716	arg1	F-O					728:730	F-O	728:730	F-O	728:730	The optimized matrices (F-O) demonstrated DEE of 91.69±1.43% and Q8h of 74.96±1.56% with minimum errors in prediction.
28482582	4	76	theme	optimized	708:716	arg1	matrices					718:725	The optimized matrices	704:725	The optimized matrices (F-O)	704:731	The optimized matrices (F-O) demonstrated DEE of 91.69±1.43% and Q8h of 74.96±1.56% with minimum errors in prediction.
28482582	3	77	theme	polymer-blend	476:488	arg1	ratios					504:509	polymer-blend (alginate:GG) ratios	476:509	polymer-blend (alginate:GG) ratios	476:509	Effects of polymer-blend (alginate:GG) ratios and crosslinker (CaCl2) concentrations on drug entrapment efficiency (DEE, %) and cumulative drug release after 8h (Q8h, %) were studied to optimize the core matrices by a 32 factorial design.
28482582	1	78	theme	gum	141:143	arg1	matrices					236:243	Novel alginate-arabic gum (AG) gel membrane coated alginate-ghatti gum (GG) modified montmorillonite (MMT) composite matrices	119:243	Novel alginate-arabic gum (AG) gel membrane coated alginate-ghatti gum (GG) modified montmorillonite (MMT) composite matrices	119:243	Novel alginate-arabic gum (AG) gel membrane coated alginate-ghatti gum (GG) modified montmorillonite (MMT) composite matrices were developed for intragastric flurbiprofen (FLU) delivery by combining floating and mucoadhesion mechanisms.
28482582	8	79	theme	developed	1366:1374	arg1	appropriate					1456:1466	appropriate	1456:1466	appropriate	1456:1466	Thus, the newly developed alginate-AG gel membrane coated alginate-GG modified MMT composite matrices are appropriate for intragastric delivery of FLU over an extended period of time with improved therapeutic benefits.
28482582	8	79	theme	developed	1366:1374	arg1	matrices					1443:1450	the newly developed alginate-AG gel membrane coated alginate-GG modified MMT composite matrices	1356:1450	the newly developed alginate-AG gel membrane coated alginate-GG modified MMT composite matrices	1356:1450	Thus, the newly developed alginate-AG gel membrane coated alginate-GG modified MMT composite matrices are appropriate for intragastric delivery of FLU over an extended period of time with improved therapeutic benefits.
28482582	3	80	theme	ratios	504:509	arg1	Effects					465:471	Effects	465:471	Effects of polymer-blend (alginate:GG) ratios and crosslinker (CaCl2) concentrations on drug entrapment efficiency (DEE, %) and cumulative drug release after 8h (Q8h, %)	465:633	Effects of polymer-blend (alginate:GG) ratios and crosslinker (CaCl2) concentrations on drug entrapment efficiency (DEE, %) and cumulative drug release after 8h (Q8h, %) were studied to optimize the core matrices by a 32 factorial design.
28482582	0	81	theme	composite	56:64	arg1	matrices					66:73	montmorillonite composite matrices	40:73	montmorillonite composite matrices for stomach-specific flurbiprofen delivery	40:116	Core-shell alginate-ghatti gum modified montmorillonite composite matrices for stomach-specific flurbiprofen delivery.
28482582	5	82	theme	release	968:974	arg1	rate					976:979	slower drug release rate	956:979	slower drug release rate	956:979	The alginate-AG gel membrane enveloped optimized matrices (F-O, coated) exhibited superior buoyancy, better ex vivo mucoadhesion and slower drug release rate.
28482582	6	83	theme	release	991:997	arg1	profile					999:1005	The drug release profile	982:1005	The drug release profile of FLU-loaded uncoated and coated optimized matrices	982:1058	The drug release profile of FLU-loaded uncoated and coated optimized matrices was best fitted in Korsmeyer-Peppas model with anomalous diffusion and case-II transport driven mechanism, respectively.
28482582	8	84	theme	gel	1388:1390	arg1	appropriate					1456:1466	appropriate	1456:1466	appropriate	1456:1466	Thus, the newly developed alginate-AG gel membrane coated alginate-GG modified MMT composite matrices are appropriate for intragastric delivery of FLU over an extended period of time with improved therapeutic benefits.
28482582	8	84	theme	gel	1388:1390	arg1	matrices					1443:1450	the newly developed alginate-AG gel membrane coated alginate-GG modified MMT composite matrices	1356:1450	the newly developed alginate-AG gel membrane coated alginate-GG modified MMT composite matrices	1356:1450	Thus, the newly developed alginate-AG gel membrane coated alginate-GG modified MMT composite matrices are appropriate for intragastric delivery of FLU over an extended period of time with improved therapeutic benefits.
28482582	2	85	theme	ionic-gelation	439:452	arg1	technique					454:462	ionic-gelation technique	439:462	ionic-gelation technique	439:462	The clay-biopolymer composite matrices containing FLU as core were accomplished by ionic-gelation technique.
28482582	7	86	theme	drug-excipients	1257:1271	arg1	compatibility					1273:1285	drug-excipients compatibility	1257:1285	drug-excipients compatibility	1257:1285	The uncoated and coated matrices containing FLU were also characterized for drug-excipients compatibility, drug crystallinity, thermal behaviour and surface morphology.
28482582	4	87	theme	%	786:786	arg1	Q8h					769:771	Q8h	769:771	Q8h	769:771	The optimized matrices (F-O) demonstrated DEE of 91.69±1.43% and Q8h of 74.96±1.56% with minimum errors in prediction.
28482582	4	87	theme	%	786:786	arg1	%					786:786	74.96±1.56%	776:786	74.96±1.56%	776:786	The optimized matrices (F-O) demonstrated DEE of 91.69±1.43% and Q8h of 74.96±1.56% with minimum errors in prediction.
28482582	4	87	theme	%	786:786	arg1	%					763:763	91.69±1.43%	753:763	91.69±1.43%	753:763	The optimized matrices (F-O) demonstrated DEE of 91.69±1.43% and Q8h of 74.96±1.56% with minimum errors in prediction.
28482582	2	88	theme	clay-biopolymer	360:374	arg1	matrices					386:393	The clay-biopolymer composite matrices	356:393	The clay-biopolymer composite matrices containing FLU as core	356:416	The clay-biopolymer composite matrices containing FLU as core were accomplished by ionic-gelation technique.
28482582	8	89	theme	modified	1420:1427	arg1	appropriate					1456:1466	appropriate	1456:1466	appropriate	1456:1466	Thus, the newly developed alginate-AG gel membrane coated alginate-GG modified MMT composite matrices are appropriate for intragastric delivery of FLU over an extended period of time with improved therapeutic benefits.
28482582	8	89	theme	modified	1420:1427	arg1	matrices					1443:1450	the newly developed alginate-AG gel membrane coated alginate-GG modified MMT composite matrices	1356:1450	the newly developed alginate-AG gel membrane coated alginate-GG modified MMT composite matrices	1356:1450	Thus, the newly developed alginate-AG gel membrane coated alginate-GG modified MMT composite matrices are appropriate for intragastric delivery of FLU over an extended period of time with improved therapeutic benefits.
28482582	3	90	theme	crosslinker	515:525	arg1	concentrations					535:548	crosslinker (CaCl2) concentrations	515:548	crosslinker (CaCl2) concentrations	515:548	Effects of polymer-blend (alginate:GG) ratios and crosslinker (CaCl2) concentrations on drug entrapment efficiency (DEE, %) and cumulative drug release after 8h (Q8h, %) were studied to optimize the core matrices by a 32 factorial design.
28482582	5	91	theme	slower	956:961	arg1	rate					976:979	slower drug release rate	956:979	slower drug release rate	956:979	The alginate-AG gel membrane enveloped optimized matrices (F-O, coated) exhibited superior buoyancy, better ex vivo mucoadhesion and slower drug release rate.
28482582	8	92	with	period	1518:1523	arg1	benefits					1559:1566	improved therapeutic benefits	1538:1566	improved therapeutic benefits	1538:1566	Thus, the newly developed alginate-AG gel membrane coated alginate-GG modified MMT composite matrices are appropriate for intragastric delivery of FLU over an extended period of time with improved therapeutic benefits.
28482582	8	93	theme	composite	1433:1441	arg1	appropriate					1456:1466	appropriate	1456:1466	appropriate	1456:1466	Thus, the newly developed alginate-AG gel membrane coated alginate-GG modified MMT composite matrices are appropriate for intragastric delivery of FLU over an extended period of time with improved therapeutic benefits.
28482582	8	93	theme	composite	1433:1441	arg1	matrices					1443:1450	the newly developed alginate-AG gel membrane coated alginate-GG modified MMT composite matrices	1356:1450	the newly developed alginate-AG gel membrane coated alginate-GG modified MMT composite matrices	1356:1450	Thus, the newly developed alginate-AG gel membrane coated alginate-GG modified MMT composite matrices are appropriate for intragastric delivery of FLU over an extended period of time with improved therapeutic benefits.
28482582	3	94	theme	drug	553:556	arg1	efficiency					569:578	drug entrapment efficiency	553:578	drug entrapment efficiency (DEE, %)	553:587	Effects of polymer-blend (alginate:GG) ratios and crosslinker (CaCl2) concentrations on drug entrapment efficiency (DEE, %) and cumulative drug release after 8h (Q8h, %) were studied to optimize the core matrices by a 32 factorial design.
24769089	3	0	theme	electron	473:480	arg1	microscopy					482:491	scanning electron microscopy	464:491	scanning electron microscopy (SEM)	464:497	Surface morphology was investigated by scanning electron microscopy (SEM).
24769089	3	0	theme	electron	473:480	arg1	SEM					494:496	SEM	494:496	SEM	494:496	Surface morphology was investigated by scanning electron microscopy (SEM).
24769089	1	1	theme	controlled	233:242	arg1	release					244:250	controlled release	233:250	controlled release of ibuprofen sodium salt (IbuNa)	233:283	Mono and multilayer composite films of poly(vinyl alcohol)-chitosan-bacterial cellulose (PVA/chitosan/BC) have been prepared to achieve controlled release of ibuprofen sodium salt (IbuNa) as model drug.
24769089	1	2	theme	vinyl	141:145	arg1	alcohol					147:153	vinyl alcohol	141:153	vinyl alcohol	141:153	Mono and multilayer composite films of poly(vinyl alcohol)-chitosan-bacterial cellulose (PVA/chitosan/BC) have been prepared to achieve controlled release of ibuprofen sodium salt (IbuNa) as model drug.
24769089	7	3	theme	BC	877:878	arg1	concentration					880:892	the BC concentration	873:892	the BC concentration	873:892	The IbuNa release rate was decreasing for all the films as the BC concentration was increased in the films composition, the decrease being higher for the multilayer films.
24769089	1	4	theme	Mono	97:100	arg1	films					127:131	Mono and multilayer composite films	97:131	films	127:131	Mono and multilayer composite films of poly(vinyl alcohol)-chitosan-bacterial cellulose (PVA/chitosan/BC) have been prepared to achieve controlled release of ibuprofen sodium salt (IbuNa) as model drug.
24769089	4	5	theme	pH	560:561	arg1	values					563:568	two different pH values	546:568	two different pH values	546:568	Equilibrium swelling was measured in water at two different pH values and in vitro release of IbuNa in pH 1.2 and pH 7.4 media was studied.
24769089	1	6	theme	ibuprofen	255:263	arg1	IbuNa					278:282	IbuNa	278:282	IbuNa	278:282	Mono and multilayer composite films of poly(vinyl alcohol)-chitosan-bacterial cellulose (PVA/chitosan/BC) have been prepared to achieve controlled release of ibuprofen sodium salt (IbuNa) as model drug.
24769089	1	6	theme	ibuprofen	255:263	arg1	salt					272:275	ibuprofen sodium salt	255:275	ibuprofen sodium salt (IbuNa)	255:283	Mono and multilayer composite films of poly(vinyl alcohol)-chitosan-bacterial cellulose (PVA/chitosan/BC) have been prepared to achieve controlled release of ibuprofen sodium salt (IbuNa) as model drug.
24769089	5	7	theme	drug	678:681	arg1	release					683:689	drug release	678:689	drug release	678:689	The release experiments revealed that drug release is pH sensitive.
24769089	6	8	theme	IbuNa	732:736	arg1	kinetics					720:727	The release kinetics	708:727	The release kinetics of IbuNa	708:736	The release kinetics of IbuNa could be described by the Fickian model of diffusion with a good agreement.
24769089	1	9	theme	-chitosan-bacterial	155:173	arg1	PVA/chitosan/BC					186:200	PVA/chitosan/BC	186:200	PVA/chitosan/BC	186:200	Mono and multilayer composite films of poly(vinyl alcohol)-chitosan-bacterial cellulose (PVA/chitosan/BC) have been prepared to achieve controlled release of ibuprofen sodium salt (IbuNa) as model drug.
24769089	1	9	theme	-chitosan-bacterial	155:173	arg1	cellulose					175:183	poly(vinyl alcohol)-chitosan-bacterial cellulose	136:183	poly(vinyl alcohol)-chitosan-bacterial cellulose (PVA/chitosan/BC)	136:201	Mono and multilayer composite films of poly(vinyl alcohol)-chitosan-bacterial cellulose (PVA/chitosan/BC) have been prepared to achieve controlled release of ibuprofen sodium salt (IbuNa) as model drug.
24769089	4	10	theme	different	550:558	arg1	values					563:568	two different pH values	546:568	two different pH values	546:568	Equilibrium swelling was measured in water at two different pH values and in vitro release of IbuNa in pH 1.2 and pH 7.4 media was studied.
24769089	2	11	theme	infrared	367:374	arg1	FTIR					390:393	FTIR	390:393	FTIR	390:393	The composite films have been characterized by Fourier transformed infrared spectroscopy (FTIR) and X-ray diffraction (XRD).
24769089	2	11	theme	infrared	367:374	arg1	spectroscopy					376:387	Fourier transformed infrared spectroscopy	347:387	Fourier transformed infrared spectroscopy (FTIR)	347:394	The composite films have been characterized by Fourier transformed infrared spectroscopy (FTIR) and X-ray diffraction (XRD).
24769089	6	12	theme	good	798:801	arg1	agreement					803:811	a good agreement	796:811	a good agreement	796:811	The release kinetics of IbuNa could be described by the Fickian model of diffusion with a good agreement.
24769089	2	13	theme	transformed	355:365	arg1	FTIR					390:393	FTIR	390:393	FTIR	390:393	The composite films have been characterized by Fourier transformed infrared spectroscopy (FTIR) and X-ray diffraction (XRD).
24769089	2	13	theme	transformed	355:365	arg1	spectroscopy					376:387	Fourier transformed infrared spectroscopy	347:387	Fourier transformed infrared spectroscopy (FTIR)	347:394	The composite films have been characterized by Fourier transformed infrared spectroscopy (FTIR) and X-ray diffraction (XRD).
24769089	0	14	theme	Composite	0:8	arg1	films					10:14	Composite films	0:14	Composite films of poly(vinyl alcohol)-chitosan-bacterial cellulose for drug	0:75	Composite films of poly(vinyl alcohol)-chitosan-bacterial cellulose for drug controlled release.
24769089	1	15	theme	sodium	265:270	arg1	IbuNa					278:282	IbuNa	278:282	IbuNa	278:282	Mono and multilayer composite films of poly(vinyl alcohol)-chitosan-bacterial cellulose (PVA/chitosan/BC) have been prepared to achieve controlled release of ibuprofen sodium salt (IbuNa) as model drug.
24769089	1	15	theme	sodium	265:270	arg1	salt					272:275	ibuprofen sodium salt	255:275	ibuprofen sodium salt (IbuNa)	255:283	Mono and multilayer composite films of poly(vinyl alcohol)-chitosan-bacterial cellulose (PVA/chitosan/BC) have been prepared to achieve controlled release of ibuprofen sodium salt (IbuNa) as model drug.
24769089	6	16	theme	release	712:718	arg1	kinetics					720:727	The release kinetics	708:727	The release kinetics of IbuNa	708:736	The release kinetics of IbuNa could be described by the Fickian model of diffusion with a good agreement.
24769089	1	17	theme	cellulose	175:183	arg1	films					127:131	Mono and multilayer composite films	97:131	films	127:131	Mono and multilayer composite films of poly(vinyl alcohol)-chitosan-bacterial cellulose (PVA/chitosan/BC) have been prepared to achieve controlled release of ibuprofen sodium salt (IbuNa) as model drug.
24769089	1	18	theme	salt	272:275	arg1	release					244:250	controlled release	233:250	controlled release of ibuprofen sodium salt (IbuNa)	233:283	Mono and multilayer composite films of poly(vinyl alcohol)-chitosan-bacterial cellulose (PVA/chitosan/BC) have been prepared to achieve controlled release of ibuprofen sodium salt (IbuNa) as model drug.
24769089	7	19	theme	multilayer	968:977	arg1	films					979:983	the multilayer films	964:983	the multilayer films	964:983	The IbuNa release rate was decreasing for all the films as the BC concentration was increased in the films composition, the decrease being higher for the multilayer films.
24769089	3	20	theme	Surface	425:431	arg1	morphology					433:442	Surface morphology	425:442	Surface morphology	425:442	Surface morphology was investigated by scanning electron microscopy (SEM).
24769089	2	21	theme	X-ray	400:404	arg1	XRD					419:421	XRD	419:421	XRD	419:421	The composite films have been characterized by Fourier transformed infrared spectroscopy (FTIR) and X-ray diffraction (XRD).
24769089	2	21	theme	X-ray	400:404	arg1	diffraction					406:416	X-ray diffraction	400:416	X-ray diffraction (XRD)	400:422	The composite films have been characterized by Fourier transformed infrared spectroscopy (FTIR) and X-ray diffraction (XRD).
24769089	0	22	dep	-chitosan-bacterial	38:56	arg1	alcohol					30:36	vinyl alcohol	24:36	vinyl alcohol	24:36	Composite films of poly(vinyl alcohol)-chitosan-bacterial cellulose for drug controlled release.
24769089	0	23	theme	vinyl	24:28	arg1	alcohol					30:36	vinyl alcohol	24:36	vinyl alcohol	24:36	Composite films of poly(vinyl alcohol)-chitosan-bacterial cellulose for drug controlled release.
24769089	2	24	theme	composite	304:312	arg1	films					314:318	The composite films	300:318	The composite films	300:318	The composite films have been characterized by Fourier transformed infrared spectroscopy (FTIR) and X-ray diffraction (XRD).
24769089	5	25	theme	release	644:650	arg1	experiments					652:662	The release experiments	640:662	The release experiments	640:662	The release experiments revealed that drug release is pH sensitive.
24769089	1	26	dep	-chitosan-bacterial	155:173	arg1	alcohol					147:153	vinyl alcohol	141:153	vinyl alcohol	141:153	Mono and multilayer composite films of poly(vinyl alcohol)-chitosan-bacterial cellulose (PVA/chitosan/BC) have been prepared to achieve controlled release of ibuprofen sodium salt (IbuNa) as model drug.
24769089	2	27	theme	Fourier	347:353	arg1	FTIR					390:393	FTIR	390:393	FTIR	390:393	The composite films have been characterized by Fourier transformed infrared spectroscopy (FTIR) and X-ray diffraction (XRD).
24769089	2	27	theme	Fourier	347:353	arg1	spectroscopy					376:387	Fourier transformed infrared spectroscopy	347:387	Fourier transformed infrared spectroscopy (FTIR)	347:394	The composite films have been characterized by Fourier transformed infrared spectroscopy (FTIR) and X-ray diffraction (XRD).
24769089	6	28	with	model	772:776	arg1	agreement					803:811	a good agreement	796:811	a good agreement	796:811	The release kinetics of IbuNa could be described by the Fickian model of diffusion with a good agreement.
24769089	7	29	theme	release	824:830	arg1	rate					832:835	The IbuNa release rate	814:835	The IbuNa release rate	814:835	The IbuNa release rate was decreasing for all the films as the BC concentration was increased in the films composition, the decrease being higher for the multilayer films.
24769089	6	30	theme	diffusion	781:789	arg1	model					772:776	the Fickian model	760:776	the Fickian model of diffusion with a good agreement	760:811	The release kinetics of IbuNa could be described by the Fickian model of diffusion with a good agreement.
24769089	4	31	theme	IbuNa	594:598	arg1	release					583:589	in vitro release	574:589	in vitro release of IbuNa in pH 1.2 and pH 7.4 media	574:625	Equilibrium swelling was measured in water at two different pH values and in vitro release of IbuNa in pH 1.2 and pH 7.4 media was studied.
24769089	4	32	theme	pH	614:615	arg1	media					621:625	pH 1.2 and pH 7.4 media	603:625	pH 1.2 and pH 7.4 media	603:625	Equilibrium swelling was measured in water at two different pH values and in vitro release of IbuNa in pH 1.2 and pH 7.4 media was studied.
24769089	1	33	theme	model	288:292	arg1	drug					294:297	model drug	288:297	model drug	288:297	Mono and multilayer composite films of poly(vinyl alcohol)-chitosan-bacterial cellulose (PVA/chitosan/BC) have been prepared to achieve controlled release of ibuprofen sodium salt (IbuNa) as model drug.
24769089	6	34	theme	Fickian	764:770	arg1	model					772:776	the Fickian model	760:776	the Fickian model of diffusion with a good agreement	760:811	The release kinetics of IbuNa could be described by the Fickian model of diffusion with a good agreement.
24769089	1	35	theme	multilayer	106:115	arg1	films					127:131	Mono and multilayer composite films	97:131	films	127:131	Mono and multilayer composite films of poly(vinyl alcohol)-chitosan-bacterial cellulose (PVA/chitosan/BC) have been prepared to achieve controlled release of ibuprofen sodium salt (IbuNa) as model drug.
24769089	4	36	theme	Equilibrium	500:510	arg1	swelling					512:519	Equilibrium swelling	500:519	Equilibrium swelling	500:519	Equilibrium swelling was measured in water at two different pH values and in vitro release of IbuNa in pH 1.2 and pH 7.4 media was studied.
24769089	4	37	dep	in	574:575	arg1	vitro					577:581	vitro	577:581	vitro	577:581	Equilibrium swelling was measured in water at two different pH values and in vitro release of IbuNa in pH 1.2 and pH 7.4 media was studied.
24769089	7	38	theme	films	915:919	arg1	composition					921:931	the films composition	911:931	the films composition	911:931	The IbuNa release rate was decreasing for all the films as the BC concentration was increased in the films composition, the decrease being higher for the multilayer films.
24769089	1	39	theme	composite	117:125	arg1	films					127:131	Mono and multilayer composite films	97:131	films	127:131	Mono and multilayer composite films of poly(vinyl alcohol)-chitosan-bacterial cellulose (PVA/chitosan/BC) have been prepared to achieve controlled release of ibuprofen sodium salt (IbuNa) as model drug.
24769089	7	40	theme	IbuNa	818:822	arg1	rate					832:835	The IbuNa release rate	814:835	The IbuNa release rate	814:835	The IbuNa release rate was decreasing for all the films as the BC concentration was increased in the films composition, the decrease being higher for the multilayer films.
24769089	0	41	theme	cellulose	58:66	arg1	films					10:14	Composite films	0:14	Composite films of poly(vinyl alcohol)-chitosan-bacterial cellulose for drug	0:75	Composite films of poly(vinyl alcohol)-chitosan-bacterial cellulose for drug controlled release.
24769089	7	42	dep	decreasing	841:850	arg1	higher					953:958	higher	953:958	higher	953:958	The IbuNa release rate was decreasing for all the films as the BC concentration was increased in the films composition, the decrease being higher for the multilayer films.
24769089	7	42	dep	decreasing	841:850	arg1	decrease					938:945	the decrease	934:945	the decrease	934:945	The IbuNa release rate was decreasing for all the films as the BC concentration was increased in the films composition, the decrease being higher for the multilayer films.
24769089	4	43	theme	pH	603:604	arg1	media					621:625	pH 1.2 and pH 7.4 media	603:625	pH 1.2 and pH 7.4 media	603:625	Equilibrium swelling was measured in water at two different pH values and in vitro release of IbuNa in pH 1.2 and pH 7.4 media was studied.
24769089	0	44	theme	-chitosan-bacterial	38:56	arg1	cellulose					58:66	poly(vinyl alcohol)-chitosan-bacterial cellulose	19:66	poly(vinyl alcohol)-chitosan-bacterial cellulose	19:66	Composite films of poly(vinyl alcohol)-chitosan-bacterial cellulose for drug controlled release.
24769089	4	45	from	release	583:589	arg1	media					621:625	pH 1.2 and pH 7.4 media	603:625	pH 1.2 and pH 7.4 media	603:625	Equilibrium swelling was measured in water at two different pH values and in vitro release of IbuNa in pH 1.2 and pH 7.4 media was studied.
24769089	3	46	theme	scanning	464:471	arg1	microscopy					482:491	scanning electron microscopy	464:491	scanning electron microscopy (SEM)	464:497	Surface morphology was investigated by scanning electron microscopy (SEM).
24769089	3	46	theme	scanning	464:471	arg1	SEM					494:496	SEM	494:496	SEM	494:496	Surface morphology was investigated by scanning electron microscopy (SEM).
24769089	4	47	theme	in	574:575	arg1	release					583:589	in vitro release	574:589	in vitro release of IbuNa in pH 1.2 and pH 7.4 media	574:625	Equilibrium swelling was measured in water at two different pH values and in vitro release of IbuNa in pH 1.2 and pH 7.4 media was studied.
29798657	12	0	theme	50	1759:1760	arg1	%					1761:1761	%	1761:1761	%	1761:1761	The proliferation test showed 25% and 50% extract of all 3 materials had significantly higher A value than control group ( P<0.05).
29798657	2	1	theme	CA+HAC	539:544	arg1	CA+HAC					539:544	CA+HAC	539:544	CA+HAC	539:544	Methods Calcium sulfate powder was mixed with HA solution, cross-linked HA solution, and phosphate buffer solution (PBS) in a ratio of 2∶1 ( W/ V) to get composites of CA+HA, CA+HAC, and CA.
29798657	2	1	theme	CA+HAC	539:544	arg1	CA					551:552	CA	551:552	CA	551:552	Methods Calcium sulfate powder was mixed with HA solution, cross-linked HA solution, and phosphate buffer solution (PBS) in a ratio of 2∶1 ( W/ V) to get composites of CA+HA, CA+HAC, and CA.
29798657	2	1	theme	CA+HAC	539:544	arg1	CA+HA					532:536	CA+HA	532:536	CA+HA	532:536	Methods Calcium sulfate powder was mixed with HA solution, cross-linked HA solution, and phosphate buffer solution (PBS) in a ratio of 2∶1 ( W/ V) to get composites of CA+HA, CA+HAC, and CA.
29798657	2	1	theme	CA+HAC	539:544	arg1	composites					518:527	composites	518:527	composites of CA+HA, CA+HAC, and CA	518:552	Methods Calcium sulfate powder was mixed with HA solution, cross-linked HA solution, and phosphate buffer solution (PBS) in a ratio of 2∶1 ( W/ V) to get composites of CA+HA, CA+HAC, and CA.
29798657	11	2	theme	biocompatibility	1437:1452	arg1	test					1454:1457	The biocompatibility test	1433:1457	The biocompatibility test	1433:1457	The biocompatibility test showed that absorbance ( A) value of CA group was significantly lower than that of control group ( P<0.05) at 6, 12, and 24 hours after culture, but no significant difference was found in A values between CA+HA group or CA+HAC group and control group ( P>0.05).
29798657	17	3	theme	CA+HA	2447:2451	arg1	group					2453:2457	CA+HA group	2447:2457	CA+HA group	2447:2457	HE staining revealed that a little bone tissue was seen in CA+HA group and CA+HAC group, but there was no bone formation in CA group at 6 weeks; more streak bone tissue in CA+HA group and CA+HAC group than CA group at 12 weeks.
29798657	12	4	theme	proliferation	1725:1737	arg1	test					1739:1742	The proliferation test	1721:1742	The proliferation test	1721:1742	The proliferation test showed 25% and 50% extract of all 3 materials had significantly higher A value than control group ( P<0.05).
29798657	11	5	from	hours	1583:1587	arg1	P<0.05					1558:1563	P<0.05	1558:1563	P<0.05	1558:1563	The biocompatibility test showed that absorbance ( A) value of CA group was significantly lower than that of control group ( P<0.05) at 6, 12, and 24 hours after culture, but no significant difference was found in A values between CA+HA group or CA+HAC group and control group ( P>0.05).
29798657	11	5	from	hours	1583:1587	arg1	group					1550:1554	control group	1542:1554	control group ( P<0.05) at 6, 12, and 24 hours after culture	1542:1601	The biocompatibility test showed that absorbance ( A) value of CA group was significantly lower than that of control group ( P<0.05) at 6, 12, and 24 hours after culture, but no significant difference was found in A values between CA+HA group or CA+HAC group and control group ( P>0.05).
29798657	3	6	used	used	648:651	arg2	extracts					568:575	The standard extracts	555:575	The standard extracts from above 3 materials	555:598	The standard extracts from above 3 materials were prepared according to ISO10993-5, and were used to culture mouse MC3T3-E1 cells.
29798657	11	7	theme	A	1484:1484	arg1	value					1487:1491	absorbance ( A) value	1471:1491	absorbance ( A) value of CA group	1471:1503	The biocompatibility test showed that absorbance ( A) value of CA group was significantly lower than that of control group ( P<0.05) at 6, 12, and 24 hours after culture, but no significant difference was found in A values between CA+HA group or CA+HAC group and control group ( P>0.05).
29798657	17	8	theme	CA+HAC	2463:2468	arg1	group					2470:2474	CA+HAC group	2463:2474	CA+HAC group	2463:2474	HE staining revealed that a little bone tissue was seen in CA+HA group and CA+HAC group, but there was no bone formation in CA group at 6 weeks; more streak bone tissue in CA+HA group and CA+HAC group than CA group at 12 weeks.
29798657	11	9	theme	absorbance	1471:1480	arg1	value					1487:1491	absorbance ( A) value	1471:1491	absorbance ( A) value of CA group	1471:1503	The biocompatibility test showed that absorbance ( A) value of CA group was significantly lower than that of control group ( P<0.05) at 6, 12, and 24 hours after culture, but no significant difference was found in A values between CA+HA group or CA+HAC group and control group ( P>0.05).
29798657	17	10	theme	streak	2538:2543	arg1	tissue					2550:2555	more streak bone tissue	2533:2555	more streak bone tissue in CA+HA group and CA+HAC group	2533:2587	HE staining revealed that a little bone tissue was seen in CA+HA group and CA+HAC group, but there was no bone formation in CA group at 6 weeks; more streak bone tissue in CA+HA group and CA+HAC group than CA group at 12 weeks.
29798657	1	11	from	biocompatibility	225:240	arg1	proliferation					278:290	cell proliferation	273:290	cell proliferation	273:290	Objective To fabricate an injectable composite bone substitute with hyaluronic acid (HA) and calcium sulfate and to evaluate the biocompatibility and effect of the composite on cell proliferation, osteogenic differentiation in vitro and osteogenic capability in vivo.
29798657	1	11	from	biocompatibility	225:240	arg1	capability					344:353	osteogenic capability	333:353	osteogenic capability	333:353	Objective To fabricate an injectable composite bone substitute with hyaluronic acid (HA) and calcium sulfate and to evaluate the biocompatibility and effect of the composite on cell proliferation, osteogenic differentiation in vitro and osteogenic capability in vivo.
29798657	1	11	from	biocompatibility	225:240	arg1	differentiation					304:318	osteogenic differentiation	293:318	osteogenic differentiation in vitro	293:327	Objective To fabricate an injectable composite bone substitute with hyaluronic acid (HA) and calcium sulfate and to evaluate the biocompatibility and effect of the composite on cell proliferation, osteogenic differentiation in vitro and osteogenic capability in vivo.
29798657	17	12	theme	CA+HAC	2576:2581	arg1	group					2583:2587	CA+HAC group	2576:2587	CA+HAC group	2576:2587	HE staining revealed that a little bone tissue was seen in CA+HA group and CA+HAC group, but there was no bone formation in CA group at 6 weeks; more streak bone tissue in CA+HA group and CA+HAC group than CA group at 12 weeks.
29798657	3	13	theme	mouse	664:668	arg1	cells					679:683	culture mouse MC3T3-E1 cells	656:683	culture mouse MC3T3-E1 cells	656:683	The standard extracts from above 3 materials were prepared according to ISO10993-5, and were used to culture mouse MC3T3-E1 cells.
29798657	14	14	theme	differentiation	2003:2017	arg1	test					2019:2022	osteogenic differentiation test	1992:2022	osteogenic differentiation test	1992:2022	And 50% extract was selected for osteogenic differentiation test.
29798657	4	15	dep	biocompatibility	700:715	arg1	The					686:688	The	686:688	The	686:688	The composite biocompatibility and cell proliferation in different concentrations of extract were tested with cell counting kit-8 (CCK-8).
29798657	16	16	theme	higher	2212:2217	arg1	BV/TV					2219:2223	higher BV/TV	2212:2223	higher BV/TV in CA+HA group and CA+HAC group	2212:2255	Micro-CT results showed higher BV/TV in CA+HA group and CA+HAC group than CA group at 6 and 12 weeks ( P<0.05), but no significant difference was found between CA+HA group and CA+HAC group ( P>0.05).
29798657	12	17	theme	A	1815:1815	arg1	value					1817:1821	significantly higher A value	1794:1821	significantly higher A value	1794:1821	The proliferation test showed 25% and 50% extract of all 3 materials had significantly higher A value than control group ( P<0.05).
29798657	17	18	theme	CA+HA	2560:2564	arg1	group					2566:2570	CA+HA group	2560:2570	CA+HA group	2560:2570	HE staining revealed that a little bone tissue was seen in CA+HA group and CA+HAC group, but there was no bone formation in CA group at 6 weeks; more streak bone tissue in CA+HA group and CA+HAC group than CA group at 12 weeks.
29798657	1	19	theme	calcium	189:195	arg1	sulfate					197:203	calcium sulfate	189:203	calcium sulfate	189:203	Objective To fabricate an injectable composite bone substitute with hyaluronic acid (HA) and calcium sulfate and to evaluate the biocompatibility and effect of the composite on cell proliferation, osteogenic differentiation in vitro and osteogenic capability in vivo.
29798657	1	20	from	effect	246:251	arg1	proliferation					278:290	cell proliferation	273:290	cell proliferation	273:290	Objective To fabricate an injectable composite bone substitute with hyaluronic acid (HA) and calcium sulfate and to evaluate the biocompatibility and effect of the composite on cell proliferation, osteogenic differentiation in vitro and osteogenic capability in vivo.
29798657	1	20	from	effect	246:251	arg1	capability					344:353	osteogenic capability	333:353	osteogenic capability	333:353	Objective To fabricate an injectable composite bone substitute with hyaluronic acid (HA) and calcium sulfate and to evaluate the biocompatibility and effect of the composite on cell proliferation, osteogenic differentiation in vitro and osteogenic capability in vivo.
29798657	1	20	from	effect	246:251	arg1	differentiation					304:318	osteogenic differentiation	293:318	osteogenic differentiation in vitro	293:327	Objective To fabricate an injectable composite bone substitute with hyaluronic acid (HA) and calcium sulfate and to evaluate the biocompatibility and effect of the composite on cell proliferation, osteogenic differentiation in vitro and osteogenic capability in vivo.
29798657	17	21	theme	bone	2494:2497	arg1	formation					2499:2507	no bone formation	2491:2507	no bone formation	2491:2507	HE staining revealed that a little bone tissue was seen in CA+HA group and CA+HAC group, but there was no bone formation in CA group at 6 weeks; more streak bone tissue in CA+HA group and CA+HAC group than CA group at 12 weeks.
29798657	9	22	theme	HE	1301:1302	arg1	staining					1304:1311	HE staining	1301:1311	HE staining	1301:1311	HE staining was used to observe bone formation.
29798657	16	23	theme	CA+HA	2348:2352	arg1	group					2354:2358	CA+HA group	2348:2358	CA+HA group	2348:2358	Micro-CT results showed higher BV/TV in CA+HA group and CA+HAC group than CA group at 6 and 12 weeks ( P<0.05), but no significant difference was found between CA+HA group and CA+HAC group ( P>0.05).
29798657	12	24	theme	materials	1780:1788	arg1	%					1753:1753	25%	1751:1753	25%	1751:1753	The proliferation test showed 25% and 50% extract of all 3 materials had significantly higher A value than control group ( P<0.05).
29798657	12	24	theme	materials	1780:1788	arg1	extract					1763:1769	50% extract	1759:1769	50% extract of all 3 materials	1759:1788	The proliferation test showed 25% and 50% extract of all 3 materials had significantly higher A value than control group ( P<0.05).
29798657	12	24	theme	materials	1780:1788	arg1	materials					1780:1788	all 3 materials	1774:1788	all 3 materials	1774:1788	The proliferation test showed 25% and 50% extract of all 3 materials had significantly higher A value than control group ( P<0.05).
29798657	10	25	dep	Results	1349:1355	arg1	Results					1349:1355	Results CA+HA and CA+HAC	1349:1372	Results CA+HA and CA+HAC	1349:1372	Results CA+HA and CA+HAC were better in injectability and stability in PBS than CA.
29798657	10	25	dep	Results	1349:1355	arg1	CA+HAC					1367:1372	CA+HAC	1367:1372	CA+HAC	1367:1372	Results CA+HA and CA+HAC were better in injectability and stability in PBS than CA.
29798657	10	25	dep	Results	1349:1355	arg1	CA+HA					1357:1361	CA+HA	1357:1361	CA+HA	1357:1361	Results CA+HA and CA+HAC were better in injectability and stability in PBS than CA.
29798657	15	26	theme	CA+HAC	2097:2102	arg1	group					2104:2108	CA+HAC group	2097:2108	CA+HAC group	2097:2108	At 14 and 21 days, ALP, COL-I and OCN concentrations of CA+HA group and CA+HAC group were significantly higher than those of CA group and control group ( P<0.05).
29798657	2	27	theme	CA+HA	532:536	arg1	CA+HAC					539:544	CA+HAC	539:544	CA+HAC	539:544	Methods Calcium sulfate powder was mixed with HA solution, cross-linked HA solution, and phosphate buffer solution (PBS) in a ratio of 2∶1 ( W/ V) to get composites of CA+HA, CA+HAC, and CA.
29798657	2	27	theme	CA+HA	532:536	arg1	CA					551:552	CA	551:552	CA	551:552	Methods Calcium sulfate powder was mixed with HA solution, cross-linked HA solution, and phosphate buffer solution (PBS) in a ratio of 2∶1 ( W/ V) to get composites of CA+HA, CA+HAC, and CA.
29798657	2	27	theme	CA+HA	532:536	arg1	CA+HA					532:536	CA+HA	532:536	CA+HA	532:536	Methods Calcium sulfate powder was mixed with HA solution, cross-linked HA solution, and phosphate buffer solution (PBS) in a ratio of 2∶1 ( W/ V) to get composites of CA+HA, CA+HAC, and CA.
29798657	2	27	theme	CA+HA	532:536	arg1	composites					518:527	composites	518:527	composites of CA+HA, CA+HAC, and CA	518:552	Methods Calcium sulfate powder was mixed with HA solution, cross-linked HA solution, and phosphate buffer solution (PBS) in a ratio of 2∶1 ( W/ V) to get composites of CA+HA, CA+HAC, and CA.
29798657	16	28	theme	CA+HAC	2244:2249	arg1	group					2251:2255	CA+HAC group	2244:2255	CA+HAC group	2244:2255	Micro-CT results showed higher BV/TV in CA+HA group and CA+HAC group than CA group at 6 and 12 weeks ( P<0.05), but no significant difference was found between CA+HA group and CA+HAC group ( P>0.05).
29798657	8	29	theme	bone	1222:1225	arg1	formation					1227:1235	new bone formation	1218:1235	new bone formation	1218:1235	Micro-CT was done to evaluate new bone formation with bone volume/tissue volume (BV/TV) ratio at 6 and 12 weeks.
29798657	11	30	theme	control	1542:1548	arg1	P<0.05					1558:1563	P<0.05	1558:1563	P<0.05	1558:1563	The biocompatibility test showed that absorbance ( A) value of CA group was significantly lower than that of control group ( P<0.05) at 6, 12, and 24 hours after culture, but no significant difference was found in A values between CA+HA group or CA+HAC group and control group ( P>0.05).
29798657	11	30	theme	control	1542:1548	arg1	group					1550:1554	control group	1542:1554	control group ( P<0.05) at 6, 12, and 24 hours after culture	1542:1601	The biocompatibility test showed that absorbance ( A) value of CA group was significantly lower than that of control group ( P<0.05) at 6, 12, and 24 hours after culture, but no significant difference was found in A values between CA+HA group or CA+HAC group and control group ( P>0.05).
29798657	4	31	from	proliferation	726:738	arg1	concentrations					753:766	different concentrations	743:766	different concentrations of extract	743:777	The composite biocompatibility and cell proliferation in different concentrations of extract were tested with cell counting kit-8 (CCK-8).
29798657	1	32	theme	composite	133:141	arg1	bone					143:146	an injectable composite bone substitute	119:157	an injectable composite bone substitute with hyaluronic acid (HA) and calcium sulfate	119:203	Objective To fabricate an injectable composite bone substitute with hyaluronic acid (HA) and calcium sulfate and to evaluate the biocompatibility and effect of the composite on cell proliferation, osteogenic differentiation in vitro and osteogenic capability in vivo.
29798657	0	33	theme	hyaluronate	56:66	arg1	hyaluronate					56:66	hyaluronate	56:66	hyaluronate	56:66	[Effect of injectable composites of calcium sulfate and hyaluronate in enhancing osteogenesis].
29798657	0	33	theme	hyaluronate	56:66	arg1	composites					22:31	injectable composites	11:31	injectable composites of calcium sulfate and hyaluronate	11:66	[Effect of injectable composites of calcium sulfate and hyaluronate in enhancing osteogenesis].
29798657	0	33	theme	hyaluronate	56:66	arg1	sulfate					44:50	calcium sulfate	36:50	calcium sulfate	36:50	[Effect of injectable composites of calcium sulfate and hyaluronate in enhancing osteogenesis].
29798657	15	34	theme	OCN	2059:2061	arg1	concentrations					2063:2076	ALP, COL-I and OCN concentrations	2044:2076	ALP, COL-I and OCN concentrations of CA+HA group and CA+HAC group	2044:2108	At 14 and 21 days, ALP, COL-I and OCN concentrations of CA+HA group and CA+HAC group were significantly higher than those of CA group and control group ( P<0.05).
29798657	9	35	theme	bone	1333:1336	arg1	formation					1338:1346	bone formation	1333:1346	bone formation	1333:1346	HE staining was used to observe bone formation.
29798657	20	36	theme	osteogenic	2860:2869	arg1	capability					2871:2880	good osteogenic capability	2855:2880	good osteogenic capability	2855:2880	They also show good osteogenic capability in vivo.
29798657	6	37	theme	osteogenic	927:936	arg1	test					954:957	osteogenic differentiation test	927:957	osteogenic differentiation test	927:957	The optimal concentration was selected for osteogenic differentiation test, and ELISA Kit was used to determine the alkaline phosphatase (ALP), collagen type I (COL-I), and osteocalcin (OCN).
29798657	11	38	located	found	1638:1642	arg1	values					1649:1654	A values	1647:1654	A values between CA+HA group or CA+HAC group and control group ( P>0.05)	1647:1718	The biocompatibility test showed that absorbance ( A) value of CA group was significantly lower than that of control group ( P<0.05) at 6, 12, and 24 hours after culture, but no significant difference was found in A values between CA+HA group or CA+HAC group and control group ( P>0.05).
29798657	11	38	located	found	1638:1642	arg2	difference					1623:1632	no significant difference	1608:1632	no significant difference	1608:1632	The biocompatibility test showed that absorbance ( A) value of CA group was significantly lower than that of control group ( P<0.05) at 6, 12, and 24 hours after culture, but no significant difference was found in A values between CA+HA group or CA+HAC group and control group ( P>0.05).
29798657	14	39	theme	50	1963:1964	arg1	%					1965:1965	%	1965:1965	%	1965:1965	And 50% extract was selected for osteogenic differentiation test.
29798657	7	40	theme	condylar	1088:1095	arg1	defect					1102:1107	The femoral condylar bone defect	1076:1107	The femoral condylar bone defect	1076:1107	The femoral condylar bone defect was made on New Zealand white rabbits and repaired with CA+HA, CA+HAC, and CA.
29798657	1	41	theme	osteogenic	333:342	arg1	capability					344:353	osteogenic capability	333:353	osteogenic capability	333:353	Objective To fabricate an injectable composite bone substitute with hyaluronic acid (HA) and calcium sulfate and to evaluate the biocompatibility and effect of the composite on cell proliferation, osteogenic differentiation in vitro and osteogenic capability in vivo.
29798657	1	42	theme	hyaluronic	164:173	arg1	HA					181:182	HA	181:182	HA	181:182	Objective To fabricate an injectable composite bone substitute with hyaluronic acid (HA) and calcium sulfate and to evaluate the biocompatibility and effect of the composite on cell proliferation, osteogenic differentiation in vitro and osteogenic capability in vivo.
29798657	1	42	theme	hyaluronic	164:173	arg1	acid					175:178	hyaluronic acid	164:178	hyaluronic acid (HA)	164:183	Objective To fabricate an injectable composite bone substitute with hyaluronic acid (HA) and calcium sulfate and to evaluate the biocompatibility and effect of the composite on cell proliferation, osteogenic differentiation in vitro and osteogenic capability in vivo.
29798657	17	43	theme	HE	2388:2389	arg1	staining					2391:2398	HE staining	2388:2398	HE staining	2388:2398	HE staining revealed that a little bone tissue was seen in CA+HA group and CA+HAC group, but there was no bone formation in CA group at 6 weeks; more streak bone tissue in CA+HA group and CA+HAC group than CA group at 12 weeks.
29798657	15	44	theme	group	2087:2091	arg1	concentrations					2063:2076	ALP, COL-I and OCN concentrations	2044:2076	ALP, COL-I and OCN concentrations of CA+HA group and CA+HAC group	2044:2108	At 14 and 21 days, ALP, COL-I and OCN concentrations of CA+HA group and CA+HAC group were significantly higher than those of CA group and control group ( P<0.05).
29798657	15	45	theme	ALP	2044:2046	arg1	concentrations					2063:2076	ALP, COL-I and OCN concentrations	2044:2076	ALP, COL-I and OCN concentrations of CA+HA group and CA+HAC group	2044:2108	At 14 and 21 days, ALP, COL-I and OCN concentrations of CA+HA group and CA+HAC group were significantly higher than those of CA group and control group ( P<0.05).
29798657	15	46	theme	CA	2150:2151	arg1	group					2153:2157	CA group	2150:2157	CA group	2150:2157	At 14 and 21 days, ALP, COL-I and OCN concentrations of CA+HA group and CA+HAC group were significantly higher than those of CA group and control group ( P<0.05).
29798657	11	47	theme	CA+HAC	1679:1684	arg1	group					1686:1690	CA+HAC group	1679:1690	CA+HAC group	1679:1690	The biocompatibility test showed that absorbance ( A) value of CA group was significantly lower than that of control group ( P<0.05) at 6, 12, and 24 hours after culture, but no significant difference was found in A values between CA+HA group or CA+HAC group and control group ( P>0.05).
29798657	4	48	theme	composite	690:698	arg1	biocompatibility					700:715	composite biocompatibility	690:715	composite biocompatibility	690:715	The composite biocompatibility and cell proliferation in different concentrations of extract were tested with cell counting kit-8 (CCK-8).
29798657	17	49	from	weeks	2609:2613	arg1	group					2597:2601	CA group	2594:2601	CA group at 12 weeks	2594:2613	HE staining revealed that a little bone tissue was seen in CA+HA group and CA+HAC group, but there was no bone formation in CA group at 6 weeks; more streak bone tissue in CA+HA group and CA+HAC group than CA group at 12 weeks.
29798657	6	50	used	used	978:981	arg2	Kit					970:972	ELISA Kit	964:972	ELISA Kit	964:972	The optimal concentration was selected for osteogenic differentiation test, and ELISA Kit was used to determine the alkaline phosphatase (ALP), collagen type I (COL-I), and osteocalcin (OCN).
29798657	2	51	theme	sulfate	380:386	arg1	powder					388:393	Methods Calcium sulfate powder	364:393	Methods Calcium sulfate powder	364:393	Methods Calcium sulfate powder was mixed with HA solution, cross-linked HA solution, and phosphate buffer solution (PBS) in a ratio of 2∶1 ( W/ V) to get composites of CA+HA, CA+HAC, and CA.
29798657	1	52	theme	composite	260:268	arg1	effect					246:251	the biocompatibility and effect	221:251	effect	246:251	Objective To fabricate an injectable composite bone substitute with hyaluronic acid (HA) and calcium sulfate and to evaluate the biocompatibility and effect of the composite on cell proliferation, osteogenic differentiation in vitro and osteogenic capability in vivo.
29798657	1	52	theme	composite	260:268	arg1	biocompatibility					225:240	the biocompatibility and effect	221:251	biocompatibility	225:240	Objective To fabricate an injectable composite bone substitute with hyaluronic acid (HA) and calcium sulfate and to evaluate the biocompatibility and effect of the composite on cell proliferation, osteogenic differentiation in vitro and osteogenic capability in vivo.
29798657	2	53	theme	cross-linked	423:434	arg1	solution					439:446	cross-linked HA solution	423:446	cross-linked HA solution	423:446	Methods Calcium sulfate powder was mixed with HA solution, cross-linked HA solution, and phosphate buffer solution (PBS) in a ratio of 2∶1 ( W/ V) to get composites of CA+HA, CA+HAC, and CA.
29798657	12	54	dep	showed	1744:1749	arg1	had					1790:1792	had	1790:1792	showed 25% and 50% extract of all 3 materials had significantly higher A value than control group ( P<0.05)	1744:1850	The proliferation test showed 25% and 50% extract of all 3 materials had significantly higher A value than control group ( P<0.05).
29798657	1	55	dep	bone	143:146	arg1	substitute					148:157	substitute	148:157	substitute	148:157	Objective To fabricate an injectable composite bone substitute with hyaluronic acid (HA) and calcium sulfate and to evaluate the biocompatibility and effect of the composite on cell proliferation, osteogenic differentiation in vitro and osteogenic capability in vivo.
29798657	0	56	theme	injectable	11:20	arg1	hyaluronate					56:66	hyaluronate	56:66	hyaluronate	56:66	[Effect of injectable composites of calcium sulfate and hyaluronate in enhancing osteogenesis].
29798657	0	56	theme	injectable	11:20	arg1	composites					22:31	injectable composites	11:31	injectable composites of calcium sulfate and hyaluronate	11:66	[Effect of injectable composites of calcium sulfate and hyaluronate in enhancing osteogenesis].
29798657	0	56	theme	injectable	11:20	arg1	sulfate					44:50	calcium sulfate	36:50	calcium sulfate	36:50	[Effect of injectable composites of calcium sulfate and hyaluronate in enhancing osteogenesis].
29798657	1	57	theme	cell	273:276	arg1	proliferation					278:290	cell proliferation	273:290	cell proliferation	273:290	Objective To fabricate an injectable composite bone substitute with hyaluronic acid (HA) and calcium sulfate and to evaluate the biocompatibility and effect of the composite on cell proliferation, osteogenic differentiation in vitro and osteogenic capability in vivo.
29798657	2	58	theme	buffer	463:468	arg1	PBS					480:482	PBS	480:482	PBS	480:482	Methods Calcium sulfate powder was mixed with HA solution, cross-linked HA solution, and phosphate buffer solution (PBS) in a ratio of 2∶1 ( W/ V) to get composites of CA+HA, CA+HAC, and CA.
29798657	2	58	theme	buffer	463:468	arg1	solution					470:477	phosphate buffer solution	453:477	phosphate buffer solution (PBS)	453:483	Methods Calcium sulfate powder was mixed with HA solution, cross-linked HA solution, and phosphate buffer solution (PBS) in a ratio of 2∶1 ( W/ V) to get composites of CA+HA, CA+HAC, and CA.
29798657	21	59	theme	bone	2933:2936	arg1	diseases					2948:2955	bone defective diseases	2933:2955	bone defective diseases	2933:2955	So it is a potential bone substitutes for bone defective diseases.
29798657	11	60	theme	group	1499:1503	arg1	value					1487:1491	absorbance ( A) value	1471:1491	absorbance ( A) value of CA group	1471:1503	The biocompatibility test showed that absorbance ( A) value of CA group was significantly lower than that of control group ( P<0.05) at 6, 12, and 24 hours after culture, but no significant difference was found in A values between CA+HA group or CA+HAC group and control group ( P>0.05).
29798657	4	61	theme	different	743:751	arg1	concentrations					753:766	different concentrations	743:766	different concentrations of extract	743:777	The composite biocompatibility and cell proliferation in different concentrations of extract were tested with cell counting kit-8 (CCK-8).
29798657	11	62	theme	CA+HA	1664:1668	arg1	group					1670:1674	CA+HA group	1664:1674	CA+HA group	1664:1674	The biocompatibility test showed that absorbance ( A) value of CA group was significantly lower than that of control group ( P<0.05) at 6, 12, and 24 hours after culture, but no significant difference was found in A values between CA+HA group or CA+HAC group and control group ( P>0.05).
29798657	16	63	theme	significant	2307:2317	arg1	difference					2319:2328	no significant difference	2304:2328	no significant difference	2304:2328	Micro-CT results showed higher BV/TV in CA+HA group and CA+HAC group than CA group at 6 and 12 weeks ( P<0.05), but no significant difference was found between CA+HA group and CA+HAC group ( P>0.05).
29798657	3	64	theme	standard	559:566	arg1	extracts					568:575	The standard extracts	555:575	The standard extracts from above 3 materials	555:598	The standard extracts from above 3 materials were prepared according to ISO10993-5, and were used to culture mouse MC3T3-E1 cells.
29798657	8	65	theme	volume/tissue	1247:1259	arg1	BV/TV					1269:1273	BV/TV	1269:1273	BV/TV	1269:1273	Micro-CT was done to evaluate new bone formation with bone volume/tissue volume (BV/TV) ratio at 6 and 12 weeks.
29798657	8	65	theme	volume/tissue	1247:1259	arg1	volume					1261:1266	bone volume/tissue volume	1242:1266	bone volume/tissue volume (BV/TV) ratio at 6 and 12 weeks	1242:1298	Micro-CT was done to evaluate new bone formation with bone volume/tissue volume (BV/TV) ratio at 6 and 12 weeks.
29798657	0	66	theme	sulfate	44:50	arg1	hyaluronate					56:66	hyaluronate	56:66	hyaluronate	56:66	[Effect of injectable composites of calcium sulfate and hyaluronate in enhancing osteogenesis].
29798657	0	66	theme	sulfate	44:50	arg1	composites					22:31	injectable composites	11:31	injectable composites of calcium sulfate and hyaluronate	11:66	[Effect of injectable composites of calcium sulfate and hyaluronate in enhancing osteogenesis].
29798657	0	66	theme	sulfate	44:50	arg1	sulfate					44:50	calcium sulfate	36:50	calcium sulfate	36:50	[Effect of injectable composites of calcium sulfate and hyaluronate in enhancing osteogenesis].
29798657	2	67	theme	CA	551:552	arg1	CA+HAC					539:544	CA+HAC	539:544	CA+HAC	539:544	Methods Calcium sulfate powder was mixed with HA solution, cross-linked HA solution, and phosphate buffer solution (PBS) in a ratio of 2∶1 ( W/ V) to get composites of CA+HA, CA+HAC, and CA.
29798657	2	67	theme	CA	551:552	arg1	CA					551:552	CA	551:552	CA	551:552	Methods Calcium sulfate powder was mixed with HA solution, cross-linked HA solution, and phosphate buffer solution (PBS) in a ratio of 2∶1 ( W/ V) to get composites of CA+HA, CA+HAC, and CA.
29798657	2	67	theme	CA	551:552	arg1	CA+HA					532:536	CA+HA	532:536	CA+HA	532:536	Methods Calcium sulfate powder was mixed with HA solution, cross-linked HA solution, and phosphate buffer solution (PBS) in a ratio of 2∶1 ( W/ V) to get composites of CA+HA, CA+HAC, and CA.
29798657	2	67	theme	CA	551:552	arg1	composites					518:527	composites	518:527	composites of CA+HA, CA+HAC, and CA	518:552	Methods Calcium sulfate powder was mixed with HA solution, cross-linked HA solution, and phosphate buffer solution (PBS) in a ratio of 2∶1 ( W/ V) to get composites of CA+HA, CA+HAC, and CA.
29798657	6	68	theme	collagen	1028:1035	arg1	COL-I					1045:1049	COL-I	1045:1049	COL-I	1045:1049	The optimal concentration was selected for osteogenic differentiation test, and ELISA Kit was used to determine the alkaline phosphatase (ALP), collagen type I (COL-I), and osteocalcin (OCN).
29798657	6	68	theme	collagen	1028:1035	arg1	type					1037:1040	collagen type I	1028:1042	collagen type I (COL-I)	1028:1050	The optimal concentration was selected for osteogenic differentiation test, and ELISA Kit was used to determine the alkaline phosphatase (ALP), collagen type I (COL-I), and osteocalcin (OCN).
29798657	21	69	theme	potential	2902:2910	arg1	bone					2912:2915	a potential bone	2900:2915	a potential bone	2900:2915	So it is a potential bone substitutes for bone defective diseases.
29798657	21	69	theme	potential	2902:2910	arg1	it					2894:2895	it	2894:2895	it	2894:2895	So it is a potential bone substitutes for bone defective diseases.
29798657	17	70	theme	bone	2423:2426	arg1	tissue					2428:2433	a little bone tissue	2414:2433	a little bone tissue	2414:2433	HE staining revealed that a little bone tissue was seen in CA+HA group and CA+HAC group, but there was no bone formation in CA group at 6 weeks; more streak bone tissue in CA+HA group and CA+HAC group than CA group at 12 weeks.
29798657	10	71	from	injectability	1389:1401	arg1	PBS					1420:1422	PBS	1420:1422	PBS	1420:1422	Results CA+HA and CA+HAC were better in injectability and stability in PBS than CA.
29798657	19	72	contain	have	2756:2759	arg1	materials					2746:2754	The materials	2742:2754	The materials	2742:2754	The materials have excellent effect on proliferation and differentiation of mouse MC3T3-E1 cells.
29798657	19	72	contain	have	2756:2759	arg2	effect					2771:2776	excellent effect	2761:2776	excellent effect	2761:2776	The materials have excellent effect on proliferation and differentiation of mouse MC3T3-E1 cells.
29798657	7	73	theme	white	1133:1137	arg1	rabbits					1139:1145	New Zealand white rabbits	1121:1145	New Zealand white rabbits	1121:1145	The femoral condylar bone defect was made on New Zealand white rabbits and repaired with CA+HA, CA+HAC, and CA.
29798657	2	74	theme	Methods	364:370	arg1	powder					388:393	Methods Calcium sulfate powder	364:393	Methods Calcium sulfate powder	364:393	Methods Calcium sulfate powder was mixed with HA solution, cross-linked HA solution, and phosphate buffer solution (PBS) in a ratio of 2∶1 ( W/ V) to get composites of CA+HA, CA+HAC, and CA.
29798657	17	75	from	tissue	2550:2555	arg1	group					2566:2570	CA+HA group	2560:2570	CA+HA group	2560:2570	HE staining revealed that a little bone tissue was seen in CA+HA group and CA+HAC group, but there was no bone formation in CA group at 6 weeks; more streak bone tissue in CA+HA group and CA+HAC group than CA group at 12 weeks.
29798657	17	75	from	tissue	2550:2555	arg1	group					2583:2587	CA+HAC group	2576:2587	CA+HAC group	2576:2587	HE staining revealed that a little bone tissue was seen in CA+HA group and CA+HAC group, but there was no bone formation in CA group at 6 weeks; more streak bone tissue in CA+HA group and CA+HAC group than CA group at 12 weeks.
29798657	13	76	theme	100	1865:1867	arg1	%					1868:1868	%	1868:1868	%	1868:1868	For 75% and 100% extract, only CA+HA group had significantly higher A value than control group ( P<0.05).
29798657	10	77	from	stability	1407:1415	arg1	PBS					1420:1422	PBS	1420:1422	PBS	1420:1422	Results CA+HA and CA+HAC were better in injectability and stability in PBS than CA.
29798657	7	78	theme	New	1121:1123	arg1	rabbits					1139:1145	New Zealand white rabbits	1121:1145	New Zealand white rabbits	1121:1145	The femoral condylar bone defect was made on New Zealand white rabbits and repaired with CA+HA, CA+HAC, and CA.
29798657	3	79	from	materials	590:598	arg1	extracts					568:575	The standard extracts	555:575	The standard extracts from above 3 materials	555:598	The standard extracts from above 3 materials were prepared according to ISO10993-5, and were used to culture mouse MC3T3-E1 cells.
29798657	11	80	theme	significant	1611:1621	arg1	difference					1623:1632	no significant difference	1608:1632	no significant difference	1608:1632	The biocompatibility test showed that absorbance ( A) value of CA group was significantly lower than that of control group ( P<0.05) at 6, 12, and 24 hours after culture, but no significant difference was found in A values between CA+HA group or CA+HAC group and control group ( P>0.05).
29798657	4	81	theme	cell	796:799	arg1	counting					801:808	cell counting kit-8	796:814	cell counting kit-8 (CCK-8)	796:822	The composite biocompatibility and cell proliferation in different concentrations of extract were tested with cell counting kit-8 (CCK-8).
29798657	4	81	theme	cell	796:799	arg1	CCK-8					817:821	CCK-8	817:821	CCK-8	817:821	The composite biocompatibility and cell proliferation in different concentrations of extract were tested with cell counting kit-8 (CCK-8).
29798657	6	82	theme	alkaline	1000:1007	arg1	ALP					1022:1024	ALP	1022:1024	ALP	1022:1024	The optimal concentration was selected for osteogenic differentiation test, and ELISA Kit was used to determine the alkaline phosphatase (ALP), collagen type I (COL-I), and osteocalcin (OCN).
29798657	6	82	theme	alkaline	1000:1007	arg1	phosphatase					1009:1019	the alkaline phosphatase	996:1019	the alkaline phosphatase (ALP)	996:1025	The optimal concentration was selected for osteogenic differentiation test, and ELISA Kit was used to determine the alkaline phosphatase (ALP), collagen type I (COL-I), and osteocalcin (OCN).
29798657	16	83	from	BV/TV	2219:2223	arg1	group					2234:2238	CA+HA group	2228:2238	CA+HA group	2228:2238	Micro-CT results showed higher BV/TV in CA+HA group and CA+HAC group than CA group at 6 and 12 weeks ( P<0.05), but no significant difference was found between CA+HA group and CA+HAC group ( P>0.05).
29798657	16	83	from	BV/TV	2219:2223	arg1	group					2251:2255	CA+HAC group	2244:2255	CA+HAC group	2244:2255	Micro-CT results showed higher BV/TV in CA+HA group and CA+HAC group than CA group at 6 and 12 weeks ( P<0.05), but no significant difference was found between CA+HA group and CA+HAC group ( P>0.05).
29798657	15	84	theme	CA+HA	2081:2085	arg1	group					2087:2091	CA+HA group	2081:2091	CA+HA group	2081:2091	At 14 and 21 days, ALP, COL-I and OCN concentrations of CA+HA group and CA+HAC group were significantly higher than those of CA group and control group ( P<0.05).
29798657	13	85	contain	had	1896:1898	arg2	value					1923:1927	significantly higher A value	1900:1927	significantly higher A value	1900:1927	For 75% and 100% extract, only CA+HA group had significantly higher A value than control group ( P<0.05).
29798657	13	85	contain	had	1896:1898	arg1	group					1890:1894	only CA+HA group	1879:1894	only CA+HA group	1879:1894	For 75% and 100% extract, only CA+HA group had significantly higher A value than control group ( P<0.05).
29798657	17	86	theme	CA	2594:2595	arg1	group					2597:2601	CA group	2594:2601	CA group at 12 weeks	2594:2613	HE staining revealed that a little bone tissue was seen in CA+HA group and CA+HAC group, but there was no bone formation in CA group at 6 weeks; more streak bone tissue in CA+HA group and CA+HAC group than CA group at 12 weeks.
29798657	20	87	theme	good	2855:2858	arg1	capability					2871:2880	good osteogenic capability	2855:2880	good osteogenic capability	2855:2880	They also show good osteogenic capability in vivo.
29798657	18	88	theme	Conclusion	2616:2625	arg1	Composites					2627:2636	Conclusion Composites	2616:2636	Conclusion Composites prepared with calcium sulfate and HA or with cross-linked HA	2616:2697	Conclusion Composites prepared with calcium sulfate and HA or with cross-linked HA are stable, injectable, and biocompatible.
29798657	4	89	theme	extract	771:777	arg1	concentrations					753:766	different concentrations	743:766	different concentrations of extract	743:777	The composite biocompatibility and cell proliferation in different concentrations of extract were tested with cell counting kit-8 (CCK-8).
29798657	12	90	theme	control	1828:1834	arg1	group					1836:1840	control group	1828:1840	control group ( P<0.05)	1828:1850	The proliferation test showed 25% and 50% extract of all 3 materials had significantly higher A value than control group ( P<0.05).
29798657	12	90	theme	control	1828:1834	arg1	P<0.05					1844:1849	P<0.05	1844:1849	P<0.05	1844:1849	The proliferation test showed 25% and 50% extract of all 3 materials had significantly higher A value than control group ( P<0.05).
29798657	19	91	theme	excellent	2761:2769	arg1	effect					2771:2776	excellent effect	2761:2776	excellent effect	2761:2776	The materials have excellent effect on proliferation and differentiation of mouse MC3T3-E1 cells.
29798657	17	92	theme	bone	2545:2548	arg1	tissue					2550:2555	more streak bone tissue	2533:2555	more streak bone tissue in CA+HA group and CA+HAC group	2533:2587	HE staining revealed that a little bone tissue was seen in CA+HA group and CA+HAC group, but there was no bone formation in CA group at 6 weeks; more streak bone tissue in CA+HA group and CA+HAC group than CA group at 12 weeks.
29798657	15	93	theme	COL-I	2049:2053	arg1	concentrations					2063:2076	ALP, COL-I and OCN concentrations	2044:2076	ALP, COL-I and OCN concentrations of CA+HA group and CA+HAC group	2044:2108	At 14 and 21 days, ALP, COL-I and OCN concentrations of CA+HA group and CA+HAC group were significantly higher than those of CA group and control group ( P<0.05).
29798657	6	94	theme	optimal	888:894	arg1	concentration					896:908	The optimal concentration	884:908	The optimal concentration	884:908	The optimal concentration was selected for osteogenic differentiation test, and ELISA Kit was used to determine the alkaline phosphatase (ALP), collagen type I (COL-I), and osteocalcin (OCN).
29798657	18	95	link	cross-linked	2683:2694	arg1	HA					2696:2697	cross-linked HA	2683:2697	cross-linked HA	2683:2697	Conclusion Composites prepared with calcium sulfate and HA or with cross-linked HA are stable, injectable, and biocompatible.
29798657	3	96	theme	MC3T3-E1	670:677	arg1	cells					679:683	culture mouse MC3T3-E1 cells	656:683	culture mouse MC3T3-E1 cells	656:683	The standard extracts from above 3 materials were prepared according to ISO10993-5, and were used to culture mouse MC3T3-E1 cells.
29798657	14	97	theme	osteogenic	1992:2001	arg1	test					2019:2022	osteogenic differentiation test	1992:2022	osteogenic differentiation test	1992:2022	And 50% extract was selected for osteogenic differentiation test.
29798657	18	98	theme	calcium	2652:2658	arg1	sulfate					2660:2666	calcium sulfate	2652:2666	calcium sulfate	2652:2666	Conclusion Composites prepared with calcium sulfate and HA or with cross-linked HA are stable, injectable, and biocompatible.
29798657	16	99	theme	CA+HA	2228:2232	arg1	group					2234:2238	CA+HA group	2228:2238	CA+HA group	2228:2238	Micro-CT results showed higher BV/TV in CA+HA group and CA+HAC group than CA group at 6 and 12 weeks ( P<0.05), but no significant difference was found between CA+HA group and CA+HAC group ( P>0.05).
29798657	13	100	theme	control	1934:1940	arg1	P<0.05					1950:1955	P<0.05	1950:1955	P<0.05	1950:1955	For 75% and 100% extract, only CA+HA group had significantly higher A value than control group ( P<0.05).
29798657	13	100	theme	control	1934:1940	arg1	group					1942:1946	control group	1934:1946	control group ( P<0.05)	1934:1956	For 75% and 100% extract, only CA+HA group had significantly higher A value than control group ( P<0.05).
29798657	12	101	theme	higher	1808:1813	arg1	value					1817:1821	significantly higher A value	1794:1821	significantly higher A value	1794:1821	The proliferation test showed 25% and 50% extract of all 3 materials had significantly higher A value than control group ( P<0.05).
29798657	3	102	theme	culture	656:662	arg1	cells					679:683	culture mouse MC3T3-E1 cells	656:683	culture mouse MC3T3-E1 cells	656:683	The standard extracts from above 3 materials were prepared according to ISO10993-5, and were used to culture mouse MC3T3-E1 cells.
29798657	11	103	theme	control	1696:1702	arg1	P>0.05					1712:1717	P>0.05	1712:1717	P>0.05	1712:1717	The biocompatibility test showed that absorbance ( A) value of CA group was significantly lower than that of control group ( P<0.05) at 6, 12, and 24 hours after culture, but no significant difference was found in A values between CA+HA group or CA+HAC group and control group ( P>0.05).
29798657	11	103	theme	control	1696:1702	arg1	group					1704:1708	control group	1696:1708	control group ( P>0.05)	1696:1718	The biocompatibility test showed that absorbance ( A) value of CA group was significantly lower than that of control group ( P<0.05) at 6, 12, and 24 hours after culture, but no significant difference was found in A values between CA+HA group or CA+HAC group and control group ( P>0.05).
29798657	2	104	theme	2∶1	499:501	arg1	ratio					490:494	a ratio	488:494	a ratio of 2∶1	488:501	Methods Calcium sulfate powder was mixed with HA solution, cross-linked HA solution, and phosphate buffer solution (PBS) in a ratio of 2∶1 ( W/ V) to get composites of CA+HA, CA+HAC, and CA.
29798657	17	105	theme	CA	2512:2513	arg1	group					2515:2519	CA group	2512:2519	CA group	2512:2519	HE staining revealed that a little bone tissue was seen in CA+HA group and CA+HAC group, but there was no bone formation in CA group at 6 weeks; more streak bone tissue in CA+HA group and CA+HAC group than CA group at 12 weeks.
29798657	15	106	theme	group	2104:2108	arg1	concentrations					2063:2076	ALP, COL-I and OCN concentrations	2044:2076	ALP, COL-I and OCN concentrations of CA+HA group and CA+HAC group	2044:2108	At 14 and 21 days, ALP, COL-I and OCN concentrations of CA+HA group and CA+HAC group were significantly higher than those of CA group and control group ( P<0.05).
29798657	1	107	theme	injectable	122:131	arg1	bone					143:146	an injectable composite bone substitute	119:157	an injectable composite bone substitute with hyaluronic acid (HA) and calcium sulfate	119:203	Objective To fabricate an injectable composite bone substitute with hyaluronic acid (HA) and calcium sulfate and to evaluate the biocompatibility and effect of the composite on cell proliferation, osteogenic differentiation in vitro and osteogenic capability in vivo.
29798657	19	108	theme	cells	2833:2837	arg1	proliferation					2781:2793	proliferation	2781:2793	proliferation	2781:2793	The materials have excellent effect on proliferation and differentiation of mouse MC3T3-E1 cells.
29798657	19	108	theme	cells	2833:2837	arg1	differentiation					2799:2813	differentiation	2799:2813	differentiation	2799:2813	The materials have excellent effect on proliferation and differentiation of mouse MC3T3-E1 cells.
29798657	7	109	theme	bone	1097:1100	arg1	defect					1102:1107	The femoral condylar bone defect	1076:1107	The femoral condylar bone defect	1076:1107	The femoral condylar bone defect was made on New Zealand white rabbits and repaired with CA+HA, CA+HAC, and CA.
29798657	13	110	theme	A	1921:1921	arg1	value					1923:1927	significantly higher A value	1900:1927	significantly higher A value	1900:1927	For 75% and 100% extract, only CA+HA group had significantly higher A value than control group ( P<0.05).
29798657	6	111	theme	differentiation	938:952	arg1	test					954:957	osteogenic differentiation test	927:957	osteogenic differentiation test	927:957	The optimal concentration was selected for osteogenic differentiation test, and ELISA Kit was used to determine the alkaline phosphatase (ALP), collagen type I (COL-I), and osteocalcin (OCN).
29798657	16	112	theme	CA+HAC	2364:2369	arg1	P>0.05					2379:2384	P>0.05	2379:2384	P>0.05	2379:2384	Micro-CT results showed higher BV/TV in CA+HA group and CA+HAC group than CA group at 6 and 12 weeks ( P<0.05), but no significant difference was found between CA+HA group and CA+HAC group ( P>0.05).
29798657	16	112	theme	CA+HAC	2364:2369	arg1	group					2371:2375	CA+HAC group	2364:2375	CA+HAC group ( P>0.05)	2364:2385	Micro-CT results showed higher BV/TV in CA+HA group and CA+HAC group than CA group at 6 and 12 weeks ( P<0.05), but no significant difference was found between CA+HA group and CA+HAC group ( P>0.05).
29798657	12	113	theme	%	1761:1761	arg1	extract					1763:1769	50% extract	1759:1769	50% extract of all 3 materials	1759:1788	The proliferation test showed 25% and 50% extract of all 3 materials had significantly higher A value than control group ( P<0.05).
29798657	14	114	theme	%	1965:1965	arg1	extract					1967:1973	50% extract	1963:1973	50% extract	1963:1973	And 50% extract was selected for osteogenic differentiation test.
29798657	19	115	theme	mouse	2818:2822	arg1	cells					2833:2837	mouse MC3T3-E1 cells	2818:2837	mouse MC3T3-E1 cells	2818:2837	The materials have excellent effect on proliferation and differentiation of mouse MC3T3-E1 cells.
29798657	7	116	theme	femoral	1080:1086	arg1	defect					1102:1107	The femoral condylar bone defect	1076:1107	The femoral condylar bone defect	1076:1107	The femoral condylar bone defect was made on New Zealand white rabbits and repaired with CA+HA, CA+HAC, and CA.
29798657	4	117	from	biocompatibility	700:715	arg1	concentrations					753:766	different concentrations	743:766	different concentrations of extract	743:777	The composite biocompatibility and cell proliferation in different concentrations of extract were tested with cell counting kit-8 (CCK-8).
29798657	9	118	used	used	1317:1320	arg2	staining					1304:1311	HE staining	1301:1311	HE staining	1301:1311	HE staining was used to observe bone formation.
29798657	16	119	theme	Micro-CT	2188:2195	arg1	results					2197:2203	Micro-CT results	2188:2203	Micro-CT results	2188:2203	Micro-CT results showed higher BV/TV in CA+HA group and CA+HAC group than CA group at 6 and 12 weeks ( P<0.05), but no significant difference was found between CA+HA group and CA+HAC group ( P>0.05).
29798657	2	120	link	cross-linked	423:434	arg1	solution					439:446	cross-linked HA solution	423:446	cross-linked HA solution	423:446	Methods Calcium sulfate powder was mixed with HA solution, cross-linked HA solution, and phosphate buffer solution (PBS) in a ratio of 2∶1 ( W/ V) to get composites of CA+HA, CA+HAC, and CA.
29798657	1	121	with	bone	143:146	arg1	HA					181:182	HA	181:182	HA	181:182	Objective To fabricate an injectable composite bone substitute with hyaluronic acid (HA) and calcium sulfate and to evaluate the biocompatibility and effect of the composite on cell proliferation, osteogenic differentiation in vitro and osteogenic capability in vivo.
29798657	1	121	with	bone	143:146	arg1	acid					175:178	hyaluronic acid	164:178	hyaluronic acid (HA)	164:183	Objective To fabricate an injectable composite bone substitute with hyaluronic acid (HA) and calcium sulfate and to evaluate the biocompatibility and effect of the composite on cell proliferation, osteogenic differentiation in vitro and osteogenic capability in vivo.
29798657	1	121	with	bone	143:146	arg1	sulfate					197:203	calcium sulfate	189:203	calcium sulfate	189:203	Objective To fabricate an injectable composite bone substitute with hyaluronic acid (HA) and calcium sulfate and to evaluate the biocompatibility and effect of the composite on cell proliferation, osteogenic differentiation in vitro and osteogenic capability in vivo.
29798657	15	122	theme	control	2163:2169	arg1	P<0.05					2179:2184	P<0.05	2179:2184	P<0.05	2179:2184	At 14 and 21 days, ALP, COL-I and OCN concentrations of CA+HA group and CA+HAC group were significantly higher than those of CA group and control group ( P<0.05).
29798657	15	122	theme	control	2163:2169	arg1	group					2171:2175	control group	2163:2175	control group ( P<0.05)	2163:2185	At 14 and 21 days, ALP, COL-I and OCN concentrations of CA+HA group and CA+HAC group were significantly higher than those of CA group and control group ( P<0.05).
29798657	8	123	from	weeks	1294:1298	arg1	ratio					1276:1280	bone volume/tissue volume (BV/TV) ratio	1242:1280	bone volume/tissue volume (BV/TV) ratio at 6 and 12 weeks	1242:1298	Micro-CT was done to evaluate new bone formation with bone volume/tissue volume (BV/TV) ratio at 6 and 12 weeks.
29798657	5	124	theme	standard	854:861	arg1	medium					863:868	standard medium	854:868	standard medium	854:868	The cells were cultured with standard medium as a control.
29798657	2	125	theme	HA	436:437	arg1	solution					439:446	cross-linked HA solution	423:446	cross-linked HA solution	423:446	Methods Calcium sulfate powder was mixed with HA solution, cross-linked HA solution, and phosphate buffer solution (PBS) in a ratio of 2∶1 ( W/ V) to get composites of CA+HA, CA+HAC, and CA.
29798657	2	126	theme	Calcium	372:378	arg1	powder					388:393	Methods Calcium sulfate powder	364:393	Methods Calcium sulfate powder	364:393	Methods Calcium sulfate powder was mixed with HA solution, cross-linked HA solution, and phosphate buffer solution (PBS) in a ratio of 2∶1 ( W/ V) to get composites of CA+HA, CA+HAC, and CA.
29798657	0	127	theme	composites	22:31	arg1	[Effect					0:6	[Effect	0:6	[Effect of injectable composites of calcium sulfate and hyaluronate in enhancing osteogenesis].	0:94	[Effect of injectable composites of calcium sulfate and hyaluronate in enhancing osteogenesis].
29798657	19	128	theme	MC3T3-E1	2824:2831	arg1	cells					2833:2837	mouse MC3T3-E1 cells	2818:2837	mouse MC3T3-E1 cells	2818:2837	The materials have excellent effect on proliferation and differentiation of mouse MC3T3-E1 cells.
29798657	11	129	theme	CA	1496:1497	arg1	group					1499:1503	CA group	1496:1503	CA group	1496:1503	The biocompatibility test showed that absorbance ( A) value of CA group was significantly lower than that of control group ( P<0.05) at 6, 12, and 24 hours after culture, but no significant difference was found in A values between CA+HA group or CA+HAC group and control group ( P>0.05).
29798657	8	130	theme	new	1218:1220	arg1	formation					1227:1235	new bone formation	1218:1235	new bone formation	1218:1235	Micro-CT was done to evaluate new bone formation with bone volume/tissue volume (BV/TV) ratio at 6 and 12 weeks.
29798657	2	131	theme	HA	410:411	arg1	solution					413:420	HA solution	410:420	HA solution	410:420	Methods Calcium sulfate powder was mixed with HA solution, cross-linked HA solution, and phosphate buffer solution (PBS) in a ratio of 2∶1 ( W/ V) to get composites of CA+HA, CA+HAC, and CA.
29798657	0	132	theme	calcium	36:42	arg1	sulfate					44:50	calcium sulfate	36:50	calcium sulfate	36:50	[Effect of injectable composites of calcium sulfate and hyaluronate in enhancing osteogenesis].
29798657	13	133	theme	higher	1914:1919	arg1	value					1923:1927	significantly higher A value	1900:1927	significantly higher A value	1900:1927	For 75% and 100% extract, only CA+HA group had significantly higher A value than control group ( P<0.05).
29798657	6	134	theme	ELISA	964:968	arg1	Kit					970:972	ELISA Kit	964:972	ELISA Kit	964:972	The optimal concentration was selected for osteogenic differentiation test, and ELISA Kit was used to determine the alkaline phosphatase (ALP), collagen type I (COL-I), and osteocalcin (OCN).
29798657	2	135	theme	phosphate	453:461	arg1	PBS					480:482	PBS	480:482	PBS	480:482	Methods Calcium sulfate powder was mixed with HA solution, cross-linked HA solution, and phosphate buffer solution (PBS) in a ratio of 2∶1 ( W/ V) to get composites of CA+HA, CA+HAC, and CA.
29798657	2	135	theme	phosphate	453:461	arg1	solution					470:477	phosphate buffer solution	453:477	phosphate buffer solution (PBS)	453:483	Methods Calcium sulfate powder was mixed with HA solution, cross-linked HA solution, and phosphate buffer solution (PBS) in a ratio of 2∶1 ( W/ V) to get composites of CA+HA, CA+HAC, and CA.
29798657	21	136	theme	defective	2938:2946	arg1	diseases					2948:2955	bone defective diseases	2933:2955	bone defective diseases	2933:2955	So it is a potential bone substitutes for bone defective diseases.
29798657	2	137	dep	mixed	399:403	arg1	W/					505:506	W/	505:506	W/	505:506	Methods Calcium sulfate powder was mixed with HA solution, cross-linked HA solution, and phosphate buffer solution (PBS) in a ratio of 2∶1 ( W/ V) to get composites of CA+HA, CA+HAC, and CA.
29798657	1	138	theme	osteogenic	293:302	arg1	differentiation					304:318	osteogenic differentiation	293:318	osteogenic differentiation in vitro	293:327	Objective To fabricate an injectable composite bone substitute with hyaluronic acid (HA) and calcium sulfate and to evaluate the biocompatibility and effect of the composite on cell proliferation, osteogenic differentiation in vitro and osteogenic capability in vivo.
29798657	16	139	theme	CA	2262:2263	arg1	group					2265:2269	CA group	2262:2269	CA group	2262:2269	Micro-CT results showed higher BV/TV in CA+HA group and CA+HAC group than CA group at 6 and 12 weeks ( P<0.05), but no significant difference was found between CA+HA group and CA+HAC group ( P>0.05).
29798657	8	140	theme	bone	1242:1245	arg1	BV/TV					1269:1273	BV/TV	1269:1273	BV/TV	1269:1273	Micro-CT was done to evaluate new bone formation with bone volume/tissue volume (BV/TV) ratio at 6 and 12 weeks.
29798657	8	140	theme	bone	1242:1245	arg1	volume					1261:1266	bone volume/tissue volume	1242:1266	bone volume/tissue volume (BV/TV) ratio at 6 and 12 weeks	1242:1298	Micro-CT was done to evaluate new bone formation with bone volume/tissue volume (BV/TV) ratio at 6 and 12 weeks.
29798657	17	141	theme	little	2416:2421	arg1	tissue					2428:2433	a little bone tissue	2414:2433	a little bone tissue	2414:2433	HE staining revealed that a little bone tissue was seen in CA+HA group and CA+HAC group, but there was no bone formation in CA group at 6 weeks; more streak bone tissue in CA+HA group and CA+HAC group than CA group at 12 weeks.
29798657	13	142	theme	CA+HA	1884:1888	arg1	group					1890:1894	only CA+HA group	1879:1894	only CA+HA group	1879:1894	For 75% and 100% extract, only CA+HA group had significantly higher A value than control group ( P<0.05).
29798657	7	143	theme	Zealand	1125:1131	arg1	rabbits					1139:1145	New Zealand white rabbits	1121:1145	New Zealand white rabbits	1121:1145	The femoral condylar bone defect was made on New Zealand white rabbits and repaired with CA+HA, CA+HAC, and CA.
29798657	8	144	theme	volume	1261:1266	arg1	ratio					1276:1280	bone volume/tissue volume (BV/TV) ratio	1242:1280	bone volume/tissue volume (BV/TV) ratio at 6 and 12 weeks	1242:1298	Micro-CT was done to evaluate new bone formation with bone volume/tissue volume (BV/TV) ratio at 6 and 12 weeks.
29798657	18	145	theme	cross-linked	2683:2694	arg1	HA					2696:2697	cross-linked HA	2683:2697	cross-linked HA	2683:2697	Conclusion Composites prepared with calcium sulfate and HA or with cross-linked HA are stable, injectable, and biocompatible.
29798657	4	146	theme	cell	721:724	arg1	proliferation					726:738	cell proliferation	721:738	cell proliferation	721:738	The composite biocompatibility and cell proliferation in different concentrations of extract were tested with cell counting kit-8 (CCK-8).
29798657	13	147	theme	%	1868:1868	arg1	extract					1870:1876	100% extract	1865:1876	100% extract	1865:1876	For 75% and 100% extract, only CA+HA group had significantly higher A value than control group ( P<0.05).
26296208	2	0	theme	newly-synthesised	492:508	arg1	proteins					510:517	newly-synthesised proteins	492:517	newly-synthesised proteins	492:517	Enzymes in this family are localised to the Golgi apparatus, and have been shown to be involved in both N- and O-linked glycosylation of newly-synthesised proteins, including cell wall glycoproteins.
26296208	2	0	theme	newly-synthesised	492:508	arg1	glycoproteins					540:552	cell wall glycoproteins	530:552	cell wall glycoproteins	530:552	Enzymes in this family are localised to the Golgi apparatus, and have been shown to be involved in both N- and O-linked glycosylation of newly-synthesised proteins, including cell wall glycoproteins.
26296208	7	1	theme	X-ray	1238:1242	arg1	crystallography					1244:1258	X-ray crystallography	1238:1258	X-ray crystallography to a resolution of 2.2 Å	1238:1283	We also present the structure of the lumenal catalytic domain of S. cerevisiae Ktr4p, determined by X-ray crystallography to a resolution of 2.2 Å, and the complex of the enzyme with GDP to 1.9 Å resolution.
26296208	5	2	theme	experimental	900:911	arg1	confirmation					913:924	experimental confirmation	900:924	experimental confirmation of the predicted glycosyltransferase activity of Ktr4p	900:979	Here we use in vitro enzyme activity assays to provide experimental confirmation of the predicted glycosyltransferase activity of Ktr4p.
26296208	3	3	from	knowledge	559:567	arg1	family					598:603	this family	593:603	this family	593:603	Our knowledge of the nine proteins in this family is however very incomplete at present.
26296208	1	4	theme	sugar	292:296	arg1	donor					298:302	the sugar donor	288:302	the sugar donor	288:302	In the yeast Saccharomyces cerevisiae, members of the Kre2/Mnt1 protein family have been shown to be α-1,2-mannosyltransferases or α-1,2-mannosylphosphate transferases, utilising an Mn2+-coordinated GDP-mannose as the sugar donor and a variety of mannose derivatives as acceptors.
26296208	2	5	from	Enzymes	355:361	arg1	family					371:376	this family	366:376	this family	366:376	Enzymes in this family are localised to the Golgi apparatus, and have been shown to be involved in both N- and O-linked glycosylation of newly-synthesised proteins, including cell wall glycoproteins.
26296208	6	6	dep	little	1079:1084	arg1	activity					1092:1099	activity	1092:1099	activity	1092:1099	Using GDP-mannose as the donor, we observe activity towards the acceptor methyl-α-mannoside, but little or no activity towards mannose or α-1,2-mannobiose.
26296208	2	7	theme	wall	535:538	arg1	glycoproteins					540:552	cell wall glycoproteins	530:552	cell wall glycoproteins	530:552	Enzymes in this family are localised to the Golgi apparatus, and have been shown to be involved in both N- and O-linked glycosylation of newly-synthesised proteins, including cell wall glycoproteins.
26296208	0	8	from	cerevisiae	62:71	arg1	Structure					0:8	Structure	0:8	Structure of the Glycosyltransferase Ktr4p from Saccharomyces cerevisiae.	0:72	Structure of the Glycosyltransferase Ktr4p from Saccharomyces cerevisiae.
26296208	5	9	theme	Ktr4p	975:979	arg1	activity					963:970	the predicted glycosyltransferase activity	929:970	the predicted glycosyltransferase activity of Ktr4p	929:979	Here we use in vitro enzyme activity assays to provide experimental confirmation of the predicted glycosyltransferase activity of Ktr4p.
26296208	2	10	gly	glycoproteins	540:552	arg1	glycoproteins					540:552	cell wall glycoproteins	530:552	cell wall glycoproteins	530:552	Enzymes in this family are localised to the Golgi apparatus, and have been shown to be involved in both N- and O-linked glycosylation of newly-synthesised proteins, including cell wall glycoproteins.
26296208	2	11	theme	cell	530:533	arg1	glycoproteins					540:552	cell wall glycoproteins	530:552	cell wall glycoproteins	530:552	Enzymes in this family are localised to the Golgi apparatus, and have been shown to be involved in both N- and O-linked glycosylation of newly-synthesised proteins, including cell wall glycoproteins.
26296208	6	12	theme	acceptor	1046:1053	arg1	methyl-α-mannoside					1055:1072	the acceptor methyl-α-mannoside	1042:1072	the acceptor methyl-α-mannoside	1042:1072	Using GDP-mannose as the donor, we observe activity towards the acceptor methyl-α-mannoside, but little or no activity towards mannose or α-1,2-mannobiose.
26296208	7	13	theme	enzyme	1309:1314	arg1	complex					1294:1300	the complex	1290:1300	the complex of the enzyme with GDP to 1.9 Å resolution	1290:1343	We also present the structure of the lumenal catalytic domain of S. cerevisiae Ktr4p, determined by X-ray crystallography to a resolution of 2.2 Å, and the complex of the enzyme with GDP to 1.9 Å resolution.
26296208	7	13	theme	enzyme	1309:1314	arg1	structure					1158:1166	the structure	1154:1166	the structure of the lumenal catalytic domain of S. cerevisiae Ktr4p, determined by X-ray crystallography to a resolution of 2.2 Å,	1154:1284	We also present the structure of the lumenal catalytic domain of S. cerevisiae Ktr4p, determined by X-ray crystallography to a resolution of 2.2 Å, and the complex of the enzyme with GDP to 1.9 Å resolution.
26296208	4	14	theme	putative	814:821	arg1	glycosyltransferases					823:842	putative glycosyltransferases	814:842	putative glycosyltransferases	814:842	Only one family member, Kre2p/Mnt1p, has been studied by structural methods, and three (Ktr4p, Ktr5p, Ktr7p) are completely uncharacterised and remain classified only as putative glycosyltransferases.
26296208	1	15	theme	yeast	81:85	arg1	cerevisiae					101:110	the yeast Saccharomyces cerevisiae	77:110	the yeast Saccharomyces cerevisiae	77:110	In the yeast Saccharomyces cerevisiae, members of the Kre2/Mnt1 protein family have been shown to be α-1,2-mannosyltransferases or α-1,2-mannosylphosphate transferases, utilising an Mn2+-coordinated GDP-mannose as the sugar donor and a variety of mannose derivatives as acceptors.
26296208	7	16	theme	domain	1193:1198	arg1	complex					1294:1300	the complex	1290:1300	the complex of the enzyme with GDP to 1.9 Å resolution	1290:1343	We also present the structure of the lumenal catalytic domain of S. cerevisiae Ktr4p, determined by X-ray crystallography to a resolution of 2.2 Å, and the complex of the enzyme with GDP to 1.9 Å resolution.
26296208	7	16	theme	domain	1193:1198	arg1	structure					1158:1166	the structure	1154:1166	the structure of the lumenal catalytic domain of S. cerevisiae Ktr4p, determined by X-ray crystallography to a resolution of 2.2 Å,	1154:1284	We also present the structure of the lumenal catalytic domain of S. cerevisiae Ktr4p, determined by X-ray crystallography to a resolution of 2.2 Å, and the complex of the enzyme with GDP to 1.9 Å resolution.
26296208	7	17	theme	lumenal	1175:1181	arg1	domain					1193:1198	the lumenal catalytic domain	1171:1198	the lumenal catalytic domain of S. cerevisiae Ktr4p, determined by X-ray crystallography to a resolution of 2.2 Å,	1171:1284	We also present the structure of the lumenal catalytic domain of S. cerevisiae Ktr4p, determined by X-ray crystallography to a resolution of 2.2 Å, and the complex of the enzyme with GDP to 1.9 Å resolution.
26296208	7	17	theme	lumenal	1175:1181	arg1	cerevisiae					1206:1215	S. cerevisiae	1203:1215	S. cerevisiae Ktr4p	1203:1221	We also present the structure of the lumenal catalytic domain of S. cerevisiae Ktr4p, determined by X-ray crystallography to a resolution of 2.2 Å, and the complex of the enzyme with GDP to 1.9 Å resolution.
26296208	1	18	theme	Saccharomyces	87:99	arg1	cerevisiae					101:110	the yeast Saccharomyces cerevisiae	77:110	the yeast Saccharomyces cerevisiae	77:110	In the yeast Saccharomyces cerevisiae, members of the Kre2/Mnt1 protein family have been shown to be α-1,2-mannosyltransferases or α-1,2-mannosylphosphate transferases, utilising an Mn2+-coordinated GDP-mannose as the sugar donor and a variety of mannose derivatives as acceptors.
26296208	3	19	from	present	635:641	arg1	incomplete					621:630	incomplete	621:630	incomplete	621:630	Our knowledge of the nine proteins in this family is however very incomplete at present.
26296208	5	20	theme	in	857:858	arg1	assays					882:887	in vitro enzyme activity assays	857:887	in vitro enzyme activity assays	857:887	Here we use in vitro enzyme activity assays to provide experimental confirmation of the predicted glycosyltransferase activity of Ktr4p.
26296208	7	21	theme	catalytic	1183:1191	arg1	domain					1193:1198	the lumenal catalytic domain	1171:1198	the lumenal catalytic domain of S. cerevisiae Ktr4p, determined by X-ray crystallography to a resolution of 2.2 Å,	1171:1284	We also present the structure of the lumenal catalytic domain of S. cerevisiae Ktr4p, determined by X-ray crystallography to a resolution of 2.2 Å, and the complex of the enzyme with GDP to 1.9 Å resolution.
26296208	7	21	theme	catalytic	1183:1191	arg1	cerevisiae					1206:1215	S. cerevisiae	1203:1215	S. cerevisiae Ktr4p	1203:1221	We also present the structure of the lumenal catalytic domain of S. cerevisiae Ktr4p, determined by X-ray crystallography to a resolution of 2.2 Å, and the complex of the enzyme with GDP to 1.9 Å resolution.
26296208	5	22	theme	predicted	933:941	arg1	activity					963:970	the predicted glycosyltransferase activity	929:970	the predicted glycosyltransferase activity of Ktr4p	929:979	Here we use in vitro enzyme activity assays to provide experimental confirmation of the predicted glycosyltransferase activity of Ktr4p.
26296208	2	23	theme	Golgi	399:403	arg1	apparatus					405:413	the Golgi apparatus	395:413	the Golgi apparatus	395:413	Enzymes in this family are localised to the Golgi apparatus, and have been shown to be involved in both N- and O-linked glycosylation of newly-synthesised proteins, including cell wall glycoproteins.
26296208	0	24	theme	Glycosyltransferase	17:35	arg1	Ktr4p					37:41	the Glycosyltransferase Ktr4p	13:41	the Glycosyltransferase Ktr4p	13:41	Structure of the Glycosyltransferase Ktr4p from Saccharomyces cerevisiae.
26296208	2	25	link	O-linked	466:473	arg1	glycosylation					475:487	O-linked glycosylation	466:487	O-linked glycosylation	466:487	Enzymes in this family are localised to the Golgi apparatus, and have been shown to be involved in both N- and O-linked glycosylation of newly-synthesised proteins, including cell wall glycoproteins.
26296208	5	26	theme	glycosyltransferase	943:961	arg1	activity					963:970	the predicted glycosyltransferase activity	929:970	the predicted glycosyltransferase activity of Ktr4p	929:979	Here we use in vitro enzyme activity assays to provide experimental confirmation of the predicted glycosyltransferase activity of Ktr4p.
26296208	1	27	theme	α-1,2-mannosylphosphate	205:227	arg1	transferases					229:240	α-1,2-mannosylphosphate transferases	205:240	α-1,2-mannosylphosphate transferases	205:240	In the yeast Saccharomyces cerevisiae, members of the Kre2/Mnt1 protein family have been shown to be α-1,2-mannosyltransferases or α-1,2-mannosylphosphate transferases, utilising an Mn2+-coordinated GDP-mannose as the sugar donor and a variety of mannose derivatives as acceptors.
26296208	5	28	theme	activity	963:970	arg1	confirmation					913:924	experimental confirmation	900:924	experimental confirmation of the predicted glycosyltransferase activity of Ktr4p	900:979	Here we use in vitro enzyme activity assays to provide experimental confirmation of the predicted glycosyltransferase activity of Ktr4p.
26296208	5	29	theme	enzyme	866:871	arg1	assays					882:887	in vitro enzyme activity assays	857:887	in vitro enzyme activity assays	857:887	Here we use in vitro enzyme activity assays to provide experimental confirmation of the predicted glycosyltransferase activity of Ktr4p.
26296208	1	30	theme	mannose	321:327	arg1	derivatives					329:339	mannose derivatives	321:339	mannose derivatives as acceptors	321:352	In the yeast Saccharomyces cerevisiae, members of the Kre2/Mnt1 protein family have been shown to be α-1,2-mannosyltransferases or α-1,2-mannosylphosphate transferases, utilising an Mn2+-coordinated GDP-mannose as the sugar donor and a variety of mannose derivatives as acceptors.
26296208	5	31	dep	in	857:858	arg1	vitro					860:864	vitro	860:864	vitro	860:864	Here we use in vitro enzyme activity assays to provide experimental confirmation of the predicted glycosyltransferase activity of Ktr4p.
26296208	4	32	theme	family	653:658	arg1	Kre2p/Mnt1p					668:678	Kre2p/Mnt1p	668:678	Kre2p/Mnt1p	668:678	Only one family member, Kre2p/Mnt1p, has been studied by structural methods, and three (Ktr4p, Ktr5p, Ktr7p) are completely uncharacterised and remain classified only as putative glycosyltransferases.
26296208	4	32	theme	family	653:658	arg1	member					660:665	Only one family member	644:665	Only one family member	644:665	Only one family member, Kre2p/Mnt1p, has been studied by structural methods, and three (Ktr4p, Ktr5p, Ktr7p) are completely uncharacterised and remain classified only as putative glycosyltransferases.
26296208	0	33	theme	Ktr4p	37:41	arg1	Structure					0:8	Structure	0:8	Structure of the Glycosyltransferase Ktr4p from Saccharomyces cerevisiae.	0:72	Structure of the Glycosyltransferase Ktr4p from Saccharomyces cerevisiae.
26296208	1	34	theme	derivatives	329:339	arg1	GDP-mannose					273:283	an Mn2+-coordinated GDP-mannose	253:283	an Mn2+-coordinated GDP-mannose as the sugar donor	253:302	In the yeast Saccharomyces cerevisiae, members of the Kre2/Mnt1 protein family have been shown to be α-1,2-mannosyltransferases or α-1,2-mannosylphosphate transferases, utilising an Mn2+-coordinated GDP-mannose as the sugar donor and a variety of mannose derivatives as acceptors.
26296208	1	34	theme	derivatives	329:339	arg1	variety					310:316	a variety	308:316	a variety of mannose derivatives as acceptors	308:352	In the yeast Saccharomyces cerevisiae, members of the Kre2/Mnt1 protein family have been shown to be α-1,2-mannosyltransferases or α-1,2-mannosylphosphate transferases, utilising an Mn2+-coordinated GDP-mannose as the sugar donor and a variety of mannose derivatives as acceptors.
26296208	1	34	theme	derivatives	329:339	arg1	derivatives					329:339	mannose derivatives	321:339	mannose derivatives as acceptors	321:352	In the yeast Saccharomyces cerevisiae, members of the Kre2/Mnt1 protein family have been shown to be α-1,2-mannosyltransferases or α-1,2-mannosylphosphate transferases, utilising an Mn2+-coordinated GDP-mannose as the sugar donor and a variety of mannose derivatives as acceptors.
26296208	2	35	theme	O-linked	466:473	arg1	glycosylation					475:487	O-linked glycosylation	466:487	O-linked glycosylation	466:487	Enzymes in this family are localised to the Golgi apparatus, and have been shown to be involved in both N- and O-linked glycosylation of newly-synthesised proteins, including cell wall glycoproteins.
26296208	2	36	gly	glycosylation	475:487	arg1	proteins					510:517	newly-synthesised proteins	492:517	newly-synthesised proteins	492:517	Enzymes in this family are localised to the Golgi apparatus, and have been shown to be involved in both N- and O-linked glycosylation of newly-synthesised proteins, including cell wall glycoproteins.
26296208	2	36	gly	glycosylation	475:487	arg1	glycoproteins					540:552	cell wall glycoproteins	530:552	cell wall glycoproteins	530:552	Enzymes in this family are localised to the Golgi apparatus, and have been shown to be involved in both N- and O-linked glycosylation of newly-synthesised proteins, including cell wall glycoproteins.
26296208	0	37	theme	Saccharomyces	48:60	arg1	cerevisiae					62:71	Saccharomyces cerevisiae	48:71	Saccharomyces cerevisiae	48:71	Structure of the Glycosyltransferase Ktr4p from Saccharomyces cerevisiae.
26296208	4	38	theme	structural	701:710	arg1	methods					712:718	structural methods	701:718	structural methods	701:718	Only one family member, Kre2p/Mnt1p, has been studied by structural methods, and three (Ktr4p, Ktr5p, Ktr7p) are completely uncharacterised and remain classified only as putative glycosyltransferases.
26296208	3	39	theme	proteins	581:588	arg1	knowledge					559:567	Our knowledge	555:567	Our knowledge of the nine proteins in this family	555:603	Our knowledge of the nine proteins in this family is however very incomplete at present.
26296208	7	40	with	enzyme	1309:1314	arg1	GDP					1321:1323	GDP	1321:1323	GDP	1321:1323	We also present the structure of the lumenal catalytic domain of S. cerevisiae Ktr4p, determined by X-ray crystallography to a resolution of 2.2 Å, and the complex of the enzyme with GDP to 1.9 Å resolution.
26296208	1	41	theme	Kre2/Mnt1	128:136	arg1	family					146:151	the Kre2/Mnt1 protein family	124:151	the Kre2/Mnt1 protein family	124:151	In the yeast Saccharomyces cerevisiae, members of the Kre2/Mnt1 protein family have been shown to be α-1,2-mannosyltransferases or α-1,2-mannosylphosphate transferases, utilising an Mn2+-coordinated GDP-mannose as the sugar donor and a variety of mannose derivatives as acceptors.
26296208	7	42	theme	Å	1332:1332	arg1	resolution					1334:1343	1.9 Å resolution	1328:1343	1.9 Å resolution	1328:1343	We also present the structure of the lumenal catalytic domain of S. cerevisiae Ktr4p, determined by X-ray crystallography to a resolution of 2.2 Å, and the complex of the enzyme with GDP to 1.9 Å resolution.
26296208	1	43	theme	protein	138:144	arg1	family					146:151	the Kre2/Mnt1 protein family	124:151	the Kre2/Mnt1 protein family	124:151	In the yeast Saccharomyces cerevisiae, members of the Kre2/Mnt1 protein family have been shown to be α-1,2-mannosyltransferases or α-1,2-mannosylphosphate transferases, utilising an Mn2+-coordinated GDP-mannose as the sugar donor and a variety of mannose derivatives as acceptors.
26296208	1	44	theme	Mn2+-coordinated	256:271	arg1	GDP-mannose					273:283	an Mn2+-coordinated GDP-mannose	253:283	an Mn2+-coordinated GDP-mannose as the sugar donor	253:302	In the yeast Saccharomyces cerevisiae, members of the Kre2/Mnt1 protein family have been shown to be α-1,2-mannosyltransferases or α-1,2-mannosylphosphate transferases, utilising an Mn2+-coordinated GDP-mannose as the sugar donor and a variety of mannose derivatives as acceptors.
26296208	1	45	theme	family	146:151	arg1	members					113:119	members	113:119	members of the Kre2/Mnt1 protein family	113:151	In the yeast Saccharomyces cerevisiae, members of the Kre2/Mnt1 protein family have been shown to be α-1,2-mannosyltransferases or α-1,2-mannosylphosphate transferases, utilising an Mn2+-coordinated GDP-mannose as the sugar donor and a variety of mannose derivatives as acceptors.
26296208	2	46	theme	proteins	510:517	arg1	N-					459:460	N-	459:460	N-	459:460	Enzymes in this family are localised to the Golgi apparatus, and have been shown to be involved in both N- and O-linked glycosylation of newly-synthesised proteins, including cell wall glycoproteins.
26296208	2	46	theme	proteins	510:517	arg1	glycosylation					475:487	O-linked glycosylation	466:487	O-linked glycosylation	466:487	Enzymes in this family are localised to the Golgi apparatus, and have been shown to be involved in both N- and O-linked glycosylation of newly-synthesised proteins, including cell wall glycoproteins.
26296208	7	47	theme	Å	1283:1283	arg1	resolution					1265:1274	a resolution	1263:1274	a resolution of 2.2 Å	1263:1283	We also present the structure of the lumenal catalytic domain of S. cerevisiae Ktr4p, determined by X-ray crystallography to a resolution of 2.2 Å, and the complex of the enzyme with GDP to 1.9 Å resolution.
26296208	5	48	theme	activity	873:880	arg1	assays					882:887	in vitro enzyme activity assays	857:887	in vitro enzyme activity assays	857:887	Here we use in vitro enzyme activity assays to provide experimental confirmation of the predicted glycosyltransferase activity of Ktr4p.
26296208	7	49	theme	cerevisiae	1206:1215	arg1	domain					1193:1198	the lumenal catalytic domain	1171:1198	the lumenal catalytic domain of S. cerevisiae Ktr4p, determined by X-ray crystallography to a resolution of 2.2 Å,	1171:1284	We also present the structure of the lumenal catalytic domain of S. cerevisiae Ktr4p, determined by X-ray crystallography to a resolution of 2.2 Å, and the complex of the enzyme with GDP to 1.9 Å resolution.
26296208	7	49	theme	cerevisiae	1206:1215	arg1	cerevisiae					1206:1215	S. cerevisiae	1203:1215	S. cerevisiae Ktr4p	1203:1221	We also present the structure of the lumenal catalytic domain of S. cerevisiae Ktr4p, determined by X-ray crystallography to a resolution of 2.2 Å, and the complex of the enzyme with GDP to 1.9 Å resolution.
25197028	0	0	theme	heparan	92:98	arg1	glycosaminoglycans					25:42	glycosaminoglycans	25:42	glycosaminoglycans	25:42	Quantitative analysis of glycosaminoglycans, chondroitin/dermatan sulfate, hyaluronic acid, heparan sulfate, and keratan sulfate by liquid chromatography-electrospray ionization-tandem mass spectrometry.
25197028	0	0	theme	heparan	92:98	arg1	sulfate					100:106	heparan sulfate	92:106	heparan sulfate	92:106	Quantitative analysis of glycosaminoglycans, chondroitin/dermatan sulfate, hyaluronic acid, heparan sulfate, and keratan sulfate by liquid chromatography-electrospray ionization-tandem mass spectrometry.
25197028	2	1	theme	heparan	655:661	arg1	sulfates					623:630	8 chondroitin/dermatan sulfates	600:630	8 chondroitin/dermatan sulfates	600:630	Using one-shot analysis with our MS/MS method, we demonstrated the simultaneous quantification of a total of 23 variously sulfated disaccharides of four GAG classes (8 chondroitin/dermatan sulfates, 1 hyaluronic acid, 12 heparan sulfates, and 2 keratan sulfates) with a sensitivity of less than 0.5 pmol within 20 min.
25197028	2	1	theme	heparan	655:661	arg1	sulfates					663:670	12 heparan sulfates	652:670	12 heparan sulfates	652:670	Using one-shot analysis with our MS/MS method, we demonstrated the simultaneous quantification of a total of 23 variously sulfated disaccharides of four GAG classes (8 chondroitin/dermatan sulfates, 1 hyaluronic acid, 12 heparan sulfates, and 2 keratan sulfates) with a sensitivity of less than 0.5 pmol within 20 min.
25197028	2	2	theme	hyaluronic	635:644	arg1	acid					646:649	1 hyaluronic acid	633:649	1 hyaluronic acid	633:649	Using one-shot analysis with our MS/MS method, we demonstrated the simultaneous quantification of a total of 23 variously sulfated disaccharides of four GAG classes (8 chondroitin/dermatan sulfates, 1 hyaluronic acid, 12 heparan sulfates, and 2 keratan sulfates) with a sensitivity of less than 0.5 pmol within 20 min.
25197028	2	2	theme	hyaluronic	635:644	arg1	sulfates					623:630	8 chondroitin/dermatan sulfates	600:630	8 chondroitin/dermatan sulfates	600:630	Using one-shot analysis with our MS/MS method, we demonstrated the simultaneous quantification of a total of 23 variously sulfated disaccharides of four GAG classes (8 chondroitin/dermatan sulfates, 1 hyaluronic acid, 12 heparan sulfates, and 2 keratan sulfates) with a sensitivity of less than 0.5 pmol within 20 min.
25197028	3	3	theme	porcine	848:854	arg1	cartilage					866:874	porcine articular cartilage	848:874	porcine articular cartilage	848:874	We showed the differences in the composition of GAG classes and the sulfation patterns between porcine articular cartilage and yellow ligament.
25197028	3	4	theme	articular	856:864	arg1	cartilage					866:874	porcine articular cartilage	848:874	porcine articular cartilage	848:874	We showed the differences in the composition of GAG classes and the sulfation patterns between porcine articular cartilage and yellow ligament.
25197028	2	5	theme	GAG	587:589	arg1	classes					591:597	four GAG classes	582:597	four GAG classes (8 chondroitin/dermatan sulfates, 1 hyaluronic acid, 12 heparan sulfates, and 2 keratan sulfates)	582:695	Using one-shot analysis with our MS/MS method, we demonstrated the simultaneous quantification of a total of 23 variously sulfated disaccharides of four GAG classes (8 chondroitin/dermatan sulfates, 1 hyaluronic acid, 12 heparan sulfates, and 2 keratan sulfates) with a sensitivity of less than 0.5 pmol within 20 min.
25197028	1	6	theme	glycosaminoglycans	407:424	arg1	analysis					395:402	simultaneous quantitative analysis	369:402	simultaneous quantitative analysis of glycosaminoglycans (GAGs)	369:431	We developed a method using liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) with a selected reaction monitoring (SRM) mode for simultaneous quantitative analysis of glycosaminoglycans (GAGs).
25197028	5	7	theme	GAGs	1175:1178	arg1	length					1165:1170	the chain length	1155:1170	the chain length of GAGs	1155:1178	The simultaneous quantification of both internal and nonreducing terminal saccharides could be useful to estimate the chain length of GAGs.
25197028	6	8	theme	"	1239:1239	arg1	analysis					1241:1248	comprehensive "GAGomic" analysis	1217:1248	comprehensive "GAGomic" analysis of biological tissues	1217:1270	This method would help to establish comprehensive "GAGomic" analysis of biological tissues.
25197028	2	9	dep	classes	591:597	arg1	acid					646:649	1 hyaluronic acid	633:649	1 hyaluronic acid	633:649	Using one-shot analysis with our MS/MS method, we demonstrated the simultaneous quantification of a total of 23 variously sulfated disaccharides of four GAG classes (8 chondroitin/dermatan sulfates, 1 hyaluronic acid, 12 heparan sulfates, and 2 keratan sulfates) with a sensitivity of less than 0.5 pmol within 20 min.
25197028	2	9	dep	classes	591:597	arg1	sulfates					663:670	12 heparan sulfates	652:670	12 heparan sulfates	652:670	Using one-shot analysis with our MS/MS method, we demonstrated the simultaneous quantification of a total of 23 variously sulfated disaccharides of four GAG classes (8 chondroitin/dermatan sulfates, 1 hyaluronic acid, 12 heparan sulfates, and 2 keratan sulfates) with a sensitivity of less than 0.5 pmol within 20 min.
25197028	2	9	dep	classes	591:597	arg1	sulfates					623:630	8 chondroitin/dermatan sulfates	600:630	8 chondroitin/dermatan sulfates	600:630	Using one-shot analysis with our MS/MS method, we demonstrated the simultaneous quantification of a total of 23 variously sulfated disaccharides of four GAG classes (8 chondroitin/dermatan sulfates, 1 hyaluronic acid, 12 heparan sulfates, and 2 keratan sulfates) with a sensitivity of less than 0.5 pmol within 20 min.
25197028	2	9	dep	classes	591:597	arg1	sulfates					687:694	2 keratan sulfates	677:694	2 keratan sulfates	677:694	Using one-shot analysis with our MS/MS method, we demonstrated the simultaneous quantification of a total of 23 variously sulfated disaccharides of four GAG classes (8 chondroitin/dermatan sulfates, 1 hyaluronic acid, 12 heparan sulfates, and 2 keratan sulfates) with a sensitivity of less than 0.5 pmol within 20 min.
25197028	6	10	theme	GAGomic	1232:1238	arg1	analysis					1241:1248	comprehensive "GAGomic" analysis	1217:1248	comprehensive "GAGomic" analysis of biological tissues	1217:1270	This method would help to establish comprehensive "GAGomic" analysis of biological tissues.
25197028	1	11	theme	selected	325:332	arg1	mode					360:363	a selected reaction monitoring (SRM) mode	323:363	a selected reaction monitoring (SRM) mode for simultaneous quantitative analysis of glycosaminoglycans (GAGs)	323:431	We developed a method using liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) with a selected reaction monitoring (SRM) mode for simultaneous quantitative analysis of glycosaminoglycans (GAGs).
25197028	0	12	theme	keratan	113:119	arg1	sulfate					121:127	keratan sulfate	113:127	keratan sulfate	113:127	Quantitative analysis of glycosaminoglycans, chondroitin/dermatan sulfate, hyaluronic acid, heparan sulfate, and keratan sulfate by liquid chromatography-electrospray ionization-tandem mass spectrometry.
25197028	0	12	theme	keratan	113:119	arg1	glycosaminoglycans					25:42	glycosaminoglycans	25:42	glycosaminoglycans	25:42	Quantitative analysis of glycosaminoglycans, chondroitin/dermatan sulfate, hyaluronic acid, heparan sulfate, and keratan sulfate by liquid chromatography-electrospray ionization-tandem mass spectrometry.
25197028	5	13	theme	chain	1159:1163	arg1	length					1165:1170	the chain length	1155:1170	the chain length of GAGs	1155:1178	The simultaneous quantification of both internal and nonreducing terminal saccharides could be useful to estimate the chain length of GAGs.
25197028	2	14	theme	keratan	679:685	arg1	sulfates					687:694	2 keratan sulfates	677:694	2 keratan sulfates	677:694	Using one-shot analysis with our MS/MS method, we demonstrated the simultaneous quantification of a total of 23 variously sulfated disaccharides of four GAG classes (8 chondroitin/dermatan sulfates, 1 hyaluronic acid, 12 heparan sulfates, and 2 keratan sulfates) with a sensitivity of less than 0.5 pmol within 20 min.
25197028	2	14	theme	keratan	679:685	arg1	sulfates					623:630	8 chondroitin/dermatan sulfates	600:630	8 chondroitin/dermatan sulfates	600:630	Using one-shot analysis with our MS/MS method, we demonstrated the simultaneous quantification of a total of 23 variously sulfated disaccharides of four GAG classes (8 chondroitin/dermatan sulfates, 1 hyaluronic acid, 12 heparan sulfates, and 2 keratan sulfates) with a sensitivity of less than 0.5 pmol within 20 min.
25197028	2	15	with	analysis	449:456	arg1	method					473:478	our MS/MS method	463:478	our MS/MS method	463:478	Using one-shot analysis with our MS/MS method, we demonstrated the simultaneous quantification of a total of 23 variously sulfated disaccharides of four GAG classes (8 chondroitin/dermatan sulfates, 1 hyaluronic acid, 12 heparan sulfates, and 2 keratan sulfates) with a sensitivity of less than 0.5 pmol within 20 min.
25197028	2	16	theme	disaccharides	565:577	arg1	total					534:538	a total	532:538	a total of 23 variously sulfated disaccharides of four GAG classes (8 chondroitin/dermatan sulfates, 1 hyaluronic acid, 12 heparan sulfates, and 2 keratan sulfates)	532:695	Using one-shot analysis with our MS/MS method, we demonstrated the simultaneous quantification of a total of 23 variously sulfated disaccharides of four GAG classes (8 chondroitin/dermatan sulfates, 1 hyaluronic acid, 12 heparan sulfates, and 2 keratan sulfates) with a sensitivity of less than 0.5 pmol within 20 min.
25197028	2	17	theme	total	534:538	arg1	quantification					514:527	the simultaneous quantification	497:527	the simultaneous quantification of a total of 23 variously sulfated disaccharides of four GAG classes (8 chondroitin/dermatan sulfates, 1 hyaluronic acid, 12 heparan sulfates, and 2 keratan sulfates)	497:695	Using one-shot analysis with our MS/MS method, we demonstrated the simultaneous quantification of a total of 23 variously sulfated disaccharides of four GAG classes (8 chondroitin/dermatan sulfates, 1 hyaluronic acid, 12 heparan sulfates, and 2 keratan sulfates) with a sensitivity of less than 0.5 pmol within 20 min.
25197028	6	18	theme	comprehensive	1217:1229	arg1	analysis					1241:1248	comprehensive "GAGomic" analysis	1217:1248	comprehensive "GAGomic" analysis of biological tissues	1217:1270	This method would help to establish comprehensive "GAGomic" analysis of biological tissues.
25197028	4	19	theme	nonreducing	990:1000	arg1	terminal					1002:1009	the nonreducing terminal	986:1009	the nonreducing terminal	986:1009	In addition to the internal disaccharides described above, some saccharides derived from the nonreducing terminal were detected simultaneously.
25197028	2	20	theme	simultaneous	501:512	arg1	quantification					514:527	the simultaneous quantification	497:527	the simultaneous quantification of a total of 23 variously sulfated disaccharides of four GAG classes (8 chondroitin/dermatan sulfates, 1 hyaluronic acid, 12 heparan sulfates, and 2 keratan sulfates)	497:695	Using one-shot analysis with our MS/MS method, we demonstrated the simultaneous quantification of a total of 23 variously sulfated disaccharides of four GAG classes (8 chondroitin/dermatan sulfates, 1 hyaluronic acid, 12 heparan sulfates, and 2 keratan sulfates) with a sensitivity of less than 0.5 pmol within 20 min.
25197028	3	21	from	differences	767:777	arg1	composition					786:796	the composition	782:796	the composition of GAG classes	782:811	We showed the differences in the composition of GAG classes and the sulfation patterns between porcine articular cartilage and yellow ligament.
25197028	3	21	from	differences	767:777	arg1	patterns					831:838	the sulfation patterns	817:838	the sulfation patterns	817:838	We showed the differences in the composition of GAG classes and the sulfation patterns between porcine articular cartilage and yellow ligament.
25197028	2	22	theme	one-shot	440:447	arg1	analysis					449:456	one-shot analysis	440:456	one-shot analysis with our MS/MS method	440:478	Using one-shot analysis with our MS/MS method, we demonstrated the simultaneous quantification of a total of 23 variously sulfated disaccharides of four GAG classes (8 chondroitin/dermatan sulfates, 1 hyaluronic acid, 12 heparan sulfates, and 2 keratan sulfates) with a sensitivity of less than 0.5 pmol within 20 min.
25197028	0	23	theme	Quantitative	0:11	arg1	analysis					13:20	Quantitative analysis	0:20	Quantitative analysis of glycosaminoglycans, chondroitin/dermatan sulfate, hyaluronic acid, heparan sulfate, and keratan sulfate by liquid chromatography-electrospray ionization-tandem mass spectrometry.	0:202	Quantitative analysis of glycosaminoglycans, chondroitin/dermatan sulfate, hyaluronic acid, heparan sulfate, and keratan sulfate by liquid chromatography-electrospray ionization-tandem mass spectrometry.
25197028	1	24	theme	reaction	334:341	arg1	mode					360:363	a selected reaction monitoring (SRM) mode	323:363	a selected reaction monitoring (SRM) mode for simultaneous quantitative analysis of glycosaminoglycans (GAGs)	323:431	We developed a method using liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) with a selected reaction monitoring (SRM) mode for simultaneous quantitative analysis of glycosaminoglycans (GAGs).
25197028	0	25	theme	glycosaminoglycans	25:42	arg1	analysis					13:20	Quantitative analysis	0:20	Quantitative analysis of glycosaminoglycans, chondroitin/dermatan sulfate, hyaluronic acid, heparan sulfate, and keratan sulfate by liquid chromatography-electrospray ionization-tandem mass spectrometry.	0:202	Quantitative analysis of glycosaminoglycans, chondroitin/dermatan sulfate, hyaluronic acid, heparan sulfate, and keratan sulfate by liquid chromatography-electrospray ionization-tandem mass spectrometry.
25197028	1	26	theme	monitoring	343:352	arg1	mode					360:363	a selected reaction monitoring (SRM) mode	323:363	a selected reaction monitoring (SRM) mode for simultaneous quantitative analysis of glycosaminoglycans (GAGs)	323:431	We developed a method using liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) with a selected reaction monitoring (SRM) mode for simultaneous quantitative analysis of glycosaminoglycans (GAGs).
25197028	2	27	theme	chondroitin/dermatan	602:621	arg1	acid					646:649	1 hyaluronic acid	633:649	1 hyaluronic acid	633:649	Using one-shot analysis with our MS/MS method, we demonstrated the simultaneous quantification of a total of 23 variously sulfated disaccharides of four GAG classes (8 chondroitin/dermatan sulfates, 1 hyaluronic acid, 12 heparan sulfates, and 2 keratan sulfates) with a sensitivity of less than 0.5 pmol within 20 min.
25197028	2	27	theme	chondroitin/dermatan	602:621	arg1	sulfates					663:670	12 heparan sulfates	652:670	12 heparan sulfates	652:670	Using one-shot analysis with our MS/MS method, we demonstrated the simultaneous quantification of a total of 23 variously sulfated disaccharides of four GAG classes (8 chondroitin/dermatan sulfates, 1 hyaluronic acid, 12 heparan sulfates, and 2 keratan sulfates) with a sensitivity of less than 0.5 pmol within 20 min.
25197028	2	27	theme	chondroitin/dermatan	602:621	arg1	sulfates					623:630	8 chondroitin/dermatan sulfates	600:630	8 chondroitin/dermatan sulfates	600:630	Using one-shot analysis with our MS/MS method, we demonstrated the simultaneous quantification of a total of 23 variously sulfated disaccharides of four GAG classes (8 chondroitin/dermatan sulfates, 1 hyaluronic acid, 12 heparan sulfates, and 2 keratan sulfates) with a sensitivity of less than 0.5 pmol within 20 min.
25197028	2	27	theme	chondroitin/dermatan	602:621	arg1	sulfates					687:694	2 keratan sulfates	677:694	2 keratan sulfates	677:694	Using one-shot analysis with our MS/MS method, we demonstrated the simultaneous quantification of a total of 23 variously sulfated disaccharides of four GAG classes (8 chondroitin/dermatan sulfates, 1 hyaluronic acid, 12 heparan sulfates, and 2 keratan sulfates) with a sensitivity of less than 0.5 pmol within 20 min.
25197028	1	28	theme	liquid	232:237	arg1	LC-ESI-MS/MS					304:315	LC-ESI-MS/MS	304:315	LC-ESI-MS/MS	304:315	We developed a method using liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) with a selected reaction monitoring (SRM) mode for simultaneous quantitative analysis of glycosaminoglycans (GAGs).
25197028	1	28	theme	liquid	232:237	arg1	spectrometry					290:301	liquid chromatography-electrospray ionization-tandem mass spectrometry	232:301	liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS)	232:316	We developed a method using liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) with a selected reaction monitoring (SRM) mode for simultaneous quantitative analysis of glycosaminoglycans (GAGs).
25197028	0	29	theme	chondroitin/dermatan	45:64	arg1	glycosaminoglycans					25:42	glycosaminoglycans	25:42	glycosaminoglycans	25:42	Quantitative analysis of glycosaminoglycans, chondroitin/dermatan sulfate, hyaluronic acid, heparan sulfate, and keratan sulfate by liquid chromatography-electrospray ionization-tandem mass spectrometry.
25197028	0	29	theme	chondroitin/dermatan	45:64	arg1	sulfate					66:72	chondroitin/dermatan sulfate	45:72	chondroitin/dermatan sulfate	45:72	Quantitative analysis of glycosaminoglycans, chondroitin/dermatan sulfate, hyaluronic acid, heparan sulfate, and keratan sulfate by liquid chromatography-electrospray ionization-tandem mass spectrometry.
25197028	1	30	theme	SRM	355:357	arg1	mode					360:363	a selected reaction monitoring (SRM) mode	323:363	a selected reaction monitoring (SRM) mode for simultaneous quantitative analysis of glycosaminoglycans (GAGs)	323:431	We developed a method using liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) with a selected reaction monitoring (SRM) mode for simultaneous quantitative analysis of glycosaminoglycans (GAGs).
25197028	0	31	theme	chromatography-electrospray	139:165	arg1	spectrometry					190:201	liquid chromatography-electrospray ionization-tandem mass spectrometry	132:201	liquid chromatography-electrospray ionization-tandem mass spectrometry	132:201	Quantitative analysis of glycosaminoglycans, chondroitin/dermatan sulfate, hyaluronic acid, heparan sulfate, and keratan sulfate by liquid chromatography-electrospray ionization-tandem mass spectrometry.
25197028	2	32	theme	MS/MS	467:471	arg1	method					473:478	our MS/MS method	463:478	our MS/MS method	463:478	Using one-shot analysis with our MS/MS method, we demonstrated the simultaneous quantification of a total of 23 variously sulfated disaccharides of four GAG classes (8 chondroitin/dermatan sulfates, 1 hyaluronic acid, 12 heparan sulfates, and 2 keratan sulfates) with a sensitivity of less than 0.5 pmol within 20 min.
25197028	1	33	theme	chromatography-electrospray	239:265	arg1	LC-ESI-MS/MS					304:315	LC-ESI-MS/MS	304:315	LC-ESI-MS/MS	304:315	We developed a method using liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) with a selected reaction monitoring (SRM) mode for simultaneous quantitative analysis of glycosaminoglycans (GAGs).
25197028	1	33	theme	chromatography-electrospray	239:265	arg1	spectrometry					290:301	liquid chromatography-electrospray ionization-tandem mass spectrometry	232:301	liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS)	232:316	We developed a method using liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) with a selected reaction monitoring (SRM) mode for simultaneous quantitative analysis of glycosaminoglycans (GAGs).
25197028	0	34	theme	liquid	132:137	arg1	spectrometry					190:201	liquid chromatography-electrospray ionization-tandem mass spectrometry	132:201	liquid chromatography-electrospray ionization-tandem mass spectrometry	132:201	Quantitative analysis of glycosaminoglycans, chondroitin/dermatan sulfate, hyaluronic acid, heparan sulfate, and keratan sulfate by liquid chromatography-electrospray ionization-tandem mass spectrometry.
25197028	1	35	theme	ionization-tandem	267:283	arg1	LC-ESI-MS/MS					304:315	LC-ESI-MS/MS	304:315	LC-ESI-MS/MS	304:315	We developed a method using liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) with a selected reaction monitoring (SRM) mode for simultaneous quantitative analysis of glycosaminoglycans (GAGs).
25197028	1	35	theme	ionization-tandem	267:283	arg1	spectrometry					290:301	liquid chromatography-electrospray ionization-tandem mass spectrometry	232:301	liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS)	232:316	We developed a method using liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) with a selected reaction monitoring (SRM) mode for simultaneous quantitative analysis of glycosaminoglycans (GAGs).
25197028	3	36	theme	GAG	801:803	arg1	classes					805:811	GAG classes	801:811	GAG classes	801:811	We showed the differences in the composition of GAG classes and the sulfation patterns between porcine articular cartilage and yellow ligament.
25197028	0	37	theme	mass	185:188	arg1	spectrometry					190:201	liquid chromatography-electrospray ionization-tandem mass spectrometry	132:201	liquid chromatography-electrospray ionization-tandem mass spectrometry	132:201	Quantitative analysis of glycosaminoglycans, chondroitin/dermatan sulfate, hyaluronic acid, heparan sulfate, and keratan sulfate by liquid chromatography-electrospray ionization-tandem mass spectrometry.
25197028	4	38	dep	disaccharides	925:937	arg1	addition					900:907	addition	900:907	addition	900:907	In addition to the internal disaccharides described above, some saccharides derived from the nonreducing terminal were detected simultaneously.
25197028	5	39	theme	internal	1081:1088	arg1	saccharides					1115:1125	both internal and nonreducing terminal saccharides	1076:1125	both internal and nonreducing terminal saccharides	1076:1125	The simultaneous quantification of both internal and nonreducing terminal saccharides could be useful to estimate the chain length of GAGs.
25197028	2	40	theme	pmol	733:736	arg1	sensitivity					704:714	a sensitivity	702:714	a sensitivity of less than 0.5 pmol	702:736	Using one-shot analysis with our MS/MS method, we demonstrated the simultaneous quantification of a total of 23 variously sulfated disaccharides of four GAG classes (8 chondroitin/dermatan sulfates, 1 hyaluronic acid, 12 heparan sulfates, and 2 keratan sulfates) with a sensitivity of less than 0.5 pmol within 20 min.
25197028	2	41	theme	classes	591:597	arg1	disaccharides					565:577	23 variously sulfated disaccharides	543:577	23 variously sulfated disaccharides of four GAG classes (8 chondroitin/dermatan sulfates, 1 hyaluronic acid, 12 heparan sulfates, and 2 keratan sulfates)	543:695	Using one-shot analysis with our MS/MS method, we demonstrated the simultaneous quantification of a total of 23 variously sulfated disaccharides of four GAG classes (8 chondroitin/dermatan sulfates, 1 hyaluronic acid, 12 heparan sulfates, and 2 keratan sulfates) with a sensitivity of less than 0.5 pmol within 20 min.
25197028	4	42	theme	internal	916:923	arg1	disaccharides					925:937	the internal disaccharides	912:937	the internal disaccharides described above	912:953	In addition to the internal disaccharides described above, some saccharides derived from the nonreducing terminal were detected simultaneously.
25197028	0	43	theme	ionization-tandem	167:183	arg1	spectrometry					190:201	liquid chromatography-electrospray ionization-tandem mass spectrometry	132:201	liquid chromatography-electrospray ionization-tandem mass spectrometry	132:201	Quantitative analysis of glycosaminoglycans, chondroitin/dermatan sulfate, hyaluronic acid, heparan sulfate, and keratan sulfate by liquid chromatography-electrospray ionization-tandem mass spectrometry.
25197028	5	44	theme	nonreducing	1094:1104	arg1	saccharides					1115:1125	both internal and nonreducing terminal saccharides	1076:1125	both internal and nonreducing terminal saccharides	1076:1125	The simultaneous quantification of both internal and nonreducing terminal saccharides could be useful to estimate the chain length of GAGs.
25197028	3	45	theme	yellow	880:885	arg1	ligament					887:894	yellow ligament	880:894	yellow ligament	880:894	We showed the differences in the composition of GAG classes and the sulfation patterns between porcine articular cartilage and yellow ligament.
25197028	1	46	theme	simultaneous	369:380	arg1	analysis					395:402	simultaneous quantitative analysis	369:402	simultaneous quantitative analysis of glycosaminoglycans (GAGs)	369:431	We developed a method using liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) with a selected reaction monitoring (SRM) mode for simultaneous quantitative analysis of glycosaminoglycans (GAGs).
25197028	5	47	theme	terminal	1106:1113	arg1	saccharides					1115:1125	both internal and nonreducing terminal saccharides	1076:1125	both internal and nonreducing terminal saccharides	1076:1125	The simultaneous quantification of both internal and nonreducing terminal saccharides could be useful to estimate the chain length of GAGs.
25197028	6	48	theme	tissues	1264:1270	arg1	analysis					1241:1248	comprehensive "GAGomic" analysis	1217:1248	comprehensive "GAGomic" analysis of biological tissues	1217:1270	This method would help to establish comprehensive "GAGomic" analysis of biological tissues.
25197028	1	49	theme	mass	285:288	arg1	LC-ESI-MS/MS					304:315	LC-ESI-MS/MS	304:315	LC-ESI-MS/MS	304:315	We developed a method using liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) with a selected reaction monitoring (SRM) mode for simultaneous quantitative analysis of glycosaminoglycans (GAGs).
25197028	1	49	theme	mass	285:288	arg1	spectrometry					290:301	liquid chromatography-electrospray ionization-tandem mass spectrometry	232:301	liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS)	232:316	We developed a method using liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) with a selected reaction monitoring (SRM) mode for simultaneous quantitative analysis of glycosaminoglycans (GAGs).
25197028	5	50	theme	saccharides	1115:1125	arg1	useful					1136:1141	useful	1136:1141	useful	1136:1141	The simultaneous quantification of both internal and nonreducing terminal saccharides could be useful to estimate the chain length of GAGs.
25197028	5	50	theme	saccharides	1115:1125	arg1	quantification					1058:1071	The simultaneous quantification	1041:1071	The simultaneous quantification of both internal and nonreducing terminal saccharides	1041:1125	The simultaneous quantification of both internal and nonreducing terminal saccharides could be useful to estimate the chain length of GAGs.
25197028	6	51	theme	biological	1253:1262	arg1	tissues					1264:1270	biological tissues	1253:1270	biological tissues	1253:1270	This method would help to establish comprehensive "GAGomic" analysis of biological tissues.
25197028	4	52	attach	derived	973:979	arg2	saccharides					961:971	some saccharides	956:971	some saccharides derived from the nonreducing terminal	956:1009	In addition to the internal disaccharides described above, some saccharides derived from the nonreducing terminal were detected simultaneously.
25197028	4	52	attach	derived	973:979	arg1	terminal					1002:1009	the nonreducing terminal	986:1009	the nonreducing terminal	986:1009	In addition to the internal disaccharides described above, some saccharides derived from the nonreducing terminal were detected simultaneously.
25197028	3	53	theme	sulfation	821:829	arg1	patterns					831:838	the sulfation patterns	817:838	the sulfation patterns	817:838	We showed the differences in the composition of GAG classes and the sulfation patterns between porcine articular cartilage and yellow ligament.
25197028	2	54	theme	sulfated	556:563	arg1	disaccharides					565:577	23 variously sulfated disaccharides	543:577	23 variously sulfated disaccharides of four GAG classes (8 chondroitin/dermatan sulfates, 1 hyaluronic acid, 12 heparan sulfates, and 2 keratan sulfates)	543:695	Using one-shot analysis with our MS/MS method, we demonstrated the simultaneous quantification of a total of 23 variously sulfated disaccharides of four GAG classes (8 chondroitin/dermatan sulfates, 1 hyaluronic acid, 12 heparan sulfates, and 2 keratan sulfates) with a sensitivity of less than 0.5 pmol within 20 min.
25197028	5	55	theme	simultaneous	1045:1056	arg1	useful					1136:1141	useful	1136:1141	useful	1136:1141	The simultaneous quantification of both internal and nonreducing terminal saccharides could be useful to estimate the chain length of GAGs.
25197028	5	55	theme	simultaneous	1045:1056	arg1	quantification					1058:1071	The simultaneous quantification	1041:1071	The simultaneous quantification of both internal and nonreducing terminal saccharides	1041:1125	The simultaneous quantification of both internal and nonreducing terminal saccharides could be useful to estimate the chain length of GAGs.
25197028	3	56	theme	classes	805:811	arg1	composition					786:796	the composition	782:796	the composition of GAG classes	782:811	We showed the differences in the composition of GAG classes and the sulfation patterns between porcine articular cartilage and yellow ligament.
25197028	3	56	theme	classes	805:811	arg1	patterns					831:838	the sulfation patterns	817:838	the sulfation patterns	817:838	We showed the differences in the composition of GAG classes and the sulfation patterns between porcine articular cartilage and yellow ligament.
25197028	0	57	theme	hyaluronic	75:84	arg1	glycosaminoglycans					25:42	glycosaminoglycans	25:42	glycosaminoglycans	25:42	Quantitative analysis of glycosaminoglycans, chondroitin/dermatan sulfate, hyaluronic acid, heparan sulfate, and keratan sulfate by liquid chromatography-electrospray ionization-tandem mass spectrometry.
25197028	0	57	theme	hyaluronic	75:84	arg1	acid					86:89	hyaluronic acid	75:89	hyaluronic acid	75:89	Quantitative analysis of glycosaminoglycans, chondroitin/dermatan sulfate, hyaluronic acid, heparan sulfate, and keratan sulfate by liquid chromatography-electrospray ionization-tandem mass spectrometry.
25197028	1	58	theme	quantitative	382:393	arg1	analysis					395:402	simultaneous quantitative analysis	369:402	simultaneous quantitative analysis of glycosaminoglycans (GAGs)	369:431	We developed a method using liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) with a selected reaction monitoring (SRM) mode for simultaneous quantitative analysis of glycosaminoglycans (GAGs).
25144681	7	0	theme	microbiota	1491:1500	arg1	composition					1502:1512	microbiota composition	1491:1512	microbiota composition in the lower GI tract	1491:1534	Each treatment group exhibited treatment-specific alterations in microbiota composition in the lower GI tract, though disease scores were only improved in VSL#3-treated animals.
25144681	3	1	theme	prior	697:701	arg1	days					692:695	10 days	689:695	10 days prior to administering one of three treatment plans	689:747	Colitis was induced in C57BL/6 mice by administering 3% DSS in drinking water for 10 days prior to administering one of three treatment plans: daily probiotic (VSL#3) supplementation for 3 days, a single tail vein injection of 1x10 (6) murine mesenchymal stem cells, or both.
25144681	1	2	theme	microbiota	287:296	arg1	dysbiosis					298:306	gastrointestinal microbiota dysbiosis	270:306	gastrointestinal microbiota dysbiosis	270:306	Inflammatory bowel diseases encompass gastrointestinal illnesses typified by chronic inflammation, loss of epithelial integrity and gastrointestinal microbiota dysbiosis.
25144681	3	3	from	DSS	663:665	arg1	water					679:683	drinking water	670:683	drinking water	670:683	Colitis was induced in C57BL/6 mice by administering 3% DSS in drinking water for 10 days prior to administering one of three treatment plans: daily probiotic (VSL#3) supplementation for 3 days, a single tail vein injection of 1x10 (6) murine mesenchymal stem cells, or both.
25144681	3	4	theme	treatment	733:741	arg1	plans					743:747	one of three treatment plans	720:747	one of three treatment plans	720:747	Colitis was induced in C57BL/6 mice by administering 3% DSS in drinking water for 10 days prior to administering one of three treatment plans: daily probiotic (VSL#3) supplementation for 3 days, a single tail vein injection of 1x10 (6) murine mesenchymal stem cells, or both.
25144681	6	5	with	composition	1311:1321	arg1	communities					1354:1364	ileal communities	1348:1364	ileal communities exhibiting the greatest change relative to control animals	1348:1423	DSS-treatment perturbed community composition in all three niches with ileal communities exhibiting the greatest change relative to control animals.
25144681	3	6	dep	induced	619:625	arg1	both					877:880	both	877:880	both	877:880	Colitis was induced in C57BL/6 mice by administering 3% DSS in drinking water for 10 days prior to administering one of three treatment plans: daily probiotic (VSL#3) supplementation for 3 days, a single tail vein injection of 1x10 (6) murine mesenchymal stem cells, or both.
25144681	3	6	dep	induced	619:625	arg1	supplementation					774:788	daily probiotic (VSL#3) supplementation	750:788	daily probiotic (VSL#3) supplementation for 3 days	750:799	Colitis was induced in C57BL/6 mice by administering 3% DSS in drinking water for 10 days prior to administering one of three treatment plans: daily probiotic (VSL#3) supplementation for 3 days, a single tail vein injection of 1x10 (6) murine mesenchymal stem cells, or both.
25144681	3	6	dep	induced	619:625	arg1	injection					821:829	a single tail vein injection	802:829	a single tail vein injection of 1x10 (6) murine mesenchymal stem cells	802:871	Colitis was induced in C57BL/6 mice by administering 3% DSS in drinking water for 10 days prior to administering one of three treatment plans: daily probiotic (VSL#3) supplementation for 3 days, a single tail vein injection of 1x10 (6) murine mesenchymal stem cells, or both.
25144681	7	7	theme	treatment	1431:1439	arg1	group					1441:1445	Each treatment group	1426:1445	Each treatment group	1426:1445	Each treatment group exhibited treatment-specific alterations in microbiota composition in the lower GI tract, though disease scores were only improved in VSL#3-treated animals.
25144681	3	8	theme	probiotic	756:764	arg1	supplementation					774:788	daily probiotic (VSL#3) supplementation	750:788	daily probiotic (VSL#3) supplementation for 3 days	750:799	Colitis was induced in C57BL/6 mice by administering 3% DSS in drinking water for 10 days prior to administering one of three treatment plans: daily probiotic (VSL#3) supplementation for 3 days, a single tail vein injection of 1x10 (6) murine mesenchymal stem cells, or both.
25144681	2	9	theme	characteristic	342:355	arg1	perturbations					357:369	these characteristic perturbations	336:369	these characteristic perturbations	336:369	In an effort to counteract these characteristic perturbations, we used stem cells and/or a probiotic therapy in a murine model of Dextran Sodium Sulfate induced colitis to examine both their efficacy in ameliorating disease and impact on niche-specific microbial communities of the lower GI tract.
25144681	2	10	theme	lower	591:595	arg1	tract					600:604	the lower GI tract	587:604	the lower GI tract	587:604	In an effort to counteract these characteristic perturbations, we used stem cells and/or a probiotic therapy in a murine model of Dextran Sodium Sulfate induced colitis to examine both their efficacy in ameliorating disease and impact on niche-specific microbial communities of the lower GI tract.
25144681	0	11	theme	ileal	108:112	arg1	composition					125:135	ileal microbiota composition	108:135	ileal microbiota composition	108:135	Amelioration of DSS-induced murine colitis by VSL#3 supplementation is primarily associated with changes in ileal microbiota composition.
25144681	4	12	theme	Ileal	883:887	arg1	sections					908:915	Ileal, cecal and colonic sections	883:915	Ileal, cecal and colonic sections	883:915	Ileal, cecal and colonic sections were collected for microbiota and histological analyses.
25144681	3	13	theme	C57BL/6	630:636	arg1	mice					638:641	C57BL/6 mice	630:641	C57BL/6 mice	630:641	Colitis was induced in C57BL/6 mice by administering 3% DSS in drinking water for 10 days prior to administering one of three treatment plans: daily probiotic (VSL#3) supplementation for 3 days, a single tail vein injection of 1x10 (6) murine mesenchymal stem cells, or both.
25144681	8	14	theme	colitic	1771:1777	arg1	mice					1779:1782	colitic mice	1771:1782	colitic mice (P<0.05)	1771:1791	The ileal microbiota were most profoundly altered in composition in this group of animals and characterized by significant Enterobacteriaceae enrichment compared with colitic mice (P<0.05).
25144681	8	14	theme	colitic	1771:1777	arg1	P<0.05					1785:1790	P<0.05	1785:1790	P<0.05	1785:1790	The ileal microbiota were most profoundly altered in composition in this group of animals and characterized by significant Enterobacteriaceae enrichment compared with colitic mice (P<0.05).
25144681	3	15	theme	VSL	767:769	arg1	#					770:770	VSL#3	767:771	VSL#3	767:771	Colitis was induced in C57BL/6 mice by administering 3% DSS in drinking water for 10 days prior to administering one of three treatment plans: daily probiotic (VSL#3) supplementation for 3 days, a single tail vein injection of 1x10 (6) murine mesenchymal stem cells, or both.
25144681	5	16	theme	distinct	1004:1011	arg1	compositions					1033:1044	distinct bacterial community compositions	1004:1044	distinct bacterial community compositions	1004:1044	Microbiota profiling revealed distinct bacterial community compositions in the ileum, cecum and colon of control untreated animals, all of which were predicted in silico to be enriched for a number of discrete KEGG pathways, indicating compositional and functional niche specificity in healthy animals.
25144681	0	17	from	changes	97:103	arg1	composition					125:135	ileal microbiota composition	108:135	ileal microbiota composition	108:135	Amelioration of DSS-induced murine colitis by VSL#3 supplementation is primarily associated with changes in ileal microbiota composition.
25144681	2	18	theme	tract	600:604	arg1	impact					537:542	impact	537:542	impact on niche-specific microbial communities of the lower GI tract	537:604	In an effort to counteract these characteristic perturbations, we used stem cells and/or a probiotic therapy in a murine model of Dextran Sodium Sulfate induced colitis to examine both their efficacy in ameliorating disease and impact on niche-specific microbial communities of the lower GI tract.
25144681	2	18	theme	tract	600:604	arg1	efficacy					500:507	their efficacy	494:507	their efficacy in ameliorating disease	494:531	In an effort to counteract these characteristic perturbations, we used stem cells and/or a probiotic therapy in a murine model of Dextran Sodium Sulfate induced colitis to examine both their efficacy in ameliorating disease and impact on niche-specific microbial communities of the lower GI tract.
25144681	2	19	from	impact	537:542	arg1	communities					572:582	niche-specific microbial communities	547:582	niche-specific microbial communities	547:582	In an effort to counteract these characteristic perturbations, we used stem cells and/or a probiotic therapy in a murine model of Dextran Sodium Sulfate induced colitis to examine both their efficacy in ameliorating disease and impact on niche-specific microbial communities of the lower GI tract.
25144681	5	20	theme	functional	1228:1237	arg1	specificity					1245:1255	compositional and functional niche specificity	1210:1255	compositional and functional niche specificity	1210:1255	Microbiota profiling revealed distinct bacterial community compositions in the ileum, cecum and colon of control untreated animals, all of which were predicted in silico to be enriched for a number of discrete KEGG pathways, indicating compositional and functional niche specificity in healthy animals.
25144681	5	21	theme	community	1023:1031	arg1	compositions					1033:1044	distinct bacterial community compositions	1004:1044	distinct bacterial community compositions	1004:1044	Microbiota profiling revealed distinct bacterial community compositions in the ileum, cecum and colon of control untreated animals, all of which were predicted in silico to be enriched for a number of discrete KEGG pathways, indicating compositional and functional niche specificity in healthy animals.
25144681	3	22	theme	vein	816:819	arg1	injection					821:829	a single tail vein injection	802:829	a single tail vein injection of 1x10 (6) murine mesenchymal stem cells	802:871	Colitis was induced in C57BL/6 mice by administering 3% DSS in drinking water for 10 days prior to administering one of three treatment plans: daily probiotic (VSL#3) supplementation for 3 days, a single tail vein injection of 1x10 (6) murine mesenchymal stem cells, or both.
25144681	7	23	theme	#	1584:1584	arg1	animals					1595:1601	VSL#3-treated animals	1581:1601	VSL#3-treated animals	1581:1601	Each treatment group exhibited treatment-specific alterations in microbiota composition in the lower GI tract, though disease scores were only improved in VSL#3-treated animals.
25144681	5	24	from	compositions	1033:1044	arg1	colon					1070:1074	colon	1070:1074	colon	1070:1074	Microbiota profiling revealed distinct bacterial community compositions in the ileum, cecum and colon of control untreated animals, all of which were predicted in silico to be enriched for a number of discrete KEGG pathways, indicating compositional and functional niche specificity in healthy animals.
25144681	5	24	from	compositions	1033:1044	arg1	cecum					1060:1064	cecum	1060:1064	cecum	1060:1064	Microbiota profiling revealed distinct bacterial community compositions in the ileum, cecum and colon of control untreated animals, all of which were predicted in silico to be enriched for a number of discrete KEGG pathways, indicating compositional and functional niche specificity in healthy animals.
25144681	5	24	from	compositions	1033:1044	arg1	ileum					1053:1057	ileum	1053:1057	ileum	1053:1057	Microbiota profiling revealed distinct bacterial community compositions in the ileum, cecum and colon of control untreated animals, all of which were predicted in silico to be enriched for a number of discrete KEGG pathways, indicating compositional and functional niche specificity in healthy animals.
25144681	5	25	theme	control	1079:1085	arg1	animals					1097:1103	control untreated animals	1079:1103	control untreated animals	1079:1103	Microbiota profiling revealed distinct bacterial community compositions in the ileum, cecum and colon of control untreated animals, all of which were predicted in silico to be enriched for a number of discrete KEGG pathways, indicating compositional and functional niche specificity in healthy animals.
25144681	1	26	theme	bowel	151:155	arg1	diseases					157:164	Inflammatory bowel diseases	138:164	Inflammatory bowel diseases	138:164	Inflammatory bowel diseases encompass gastrointestinal illnesses typified by chronic inflammation, loss of epithelial integrity and gastrointestinal microbiota dysbiosis.
25144681	3	27	theme	single	804:809	arg1	injection					821:829	a single tail vein injection	802:829	a single tail vein injection of 1x10 (6) murine mesenchymal stem cells	802:871	Colitis was induced in C57BL/6 mice by administering 3% DSS in drinking water for 10 days prior to administering one of three treatment plans: daily probiotic (VSL#3) supplementation for 3 days, a single tail vein injection of 1x10 (6) murine mesenchymal stem cells, or both.
25144681	3	28	dep	probiotic	756:764	arg1	#					770:770	VSL#3	767:771	VSL#3	767:771	Colitis was induced in C57BL/6 mice by administering 3% DSS in drinking water for 10 days prior to administering one of three treatment plans: daily probiotic (VSL#3) supplementation for 3 days, a single tail vein injection of 1x10 (6) murine mesenchymal stem cells, or both.
25144681	2	29	theme	microbial	562:570	arg1	communities					572:582	niche-specific microbial communities	547:582	niche-specific microbial communities	547:582	In an effort to counteract these characteristic perturbations, we used stem cells and/or a probiotic therapy in a murine model of Dextran Sodium Sulfate induced colitis to examine both their efficacy in ameliorating disease and impact on niche-specific microbial communities of the lower GI tract.
25144681	5	30	theme	compositional	1210:1222	arg1	specificity					1245:1255	compositional and functional niche specificity	1210:1255	compositional and functional niche specificity	1210:1255	Microbiota profiling revealed distinct bacterial community compositions in the ileum, cecum and colon of control untreated animals, all of which were predicted in silico to be enriched for a number of discrete KEGG pathways, indicating compositional and functional niche specificity in healthy animals.
25144681	3	31	theme	cells	867:871	arg1	supplementation					774:788	daily probiotic (VSL#3) supplementation	750:788	daily probiotic (VSL#3) supplementation for 3 days	750:799	Colitis was induced in C57BL/6 mice by administering 3% DSS in drinking water for 10 days prior to administering one of three treatment plans: daily probiotic (VSL#3) supplementation for 3 days, a single tail vein injection of 1x10 (6) murine mesenchymal stem cells, or both.
25144681	3	31	theme	cells	867:871	arg1	injection					821:829	a single tail vein injection	802:829	a single tail vein injection of 1x10 (6) murine mesenchymal stem cells	802:871	Colitis was induced in C57BL/6 mice by administering 3% DSS in drinking water for 10 days prior to administering one of three treatment plans: daily probiotic (VSL#3) supplementation for 3 days, a single tail vein injection of 1x10 (6) murine mesenchymal stem cells, or both.
25144681	4	32	theme	colonic	900:906	arg1	sections					908:915	Ileal, cecal and colonic sections	883:915	Ileal, cecal and colonic sections	883:915	Ileal, cecal and colonic sections were collected for microbiota and histological analyses.
25144681	2	33	theme	murine	423:428	arg1	model					430:434	a murine model	421:434	a murine model of Dextran Sodium Sulfate induced colitis	421:476	In an effort to counteract these characteristic perturbations, we used stem cells and/or a probiotic therapy in a murine model of Dextran Sodium Sulfate induced colitis to examine both their efficacy in ameliorating disease and impact on niche-specific microbial communities of the lower GI tract.
25144681	3	34	theme	mesenchymal	850:860	arg1	cells					867:871	1x10 (6) murine mesenchymal stem cells	834:871	1x10 (6) murine mesenchymal stem cells	834:871	Colitis was induced in C57BL/6 mice by administering 3% DSS in drinking water for 10 days prior to administering one of three treatment plans: daily probiotic (VSL#3) supplementation for 3 days, a single tail vein injection of 1x10 (6) murine mesenchymal stem cells, or both.
25144681	5	35	theme	healthy	1260:1266	arg1	animals					1268:1274	healthy animals	1260:1274	healthy animals	1260:1274	Microbiota profiling revealed distinct bacterial community compositions in the ileum, cecum and colon of control untreated animals, all of which were predicted in silico to be enriched for a number of discrete KEGG pathways, indicating compositional and functional niche specificity in healthy animals.
25144681	2	36	theme	Sodium	447:452	arg1	Sulfate					454:460	Dextran Sodium Sulfate	439:460	Dextran Sodium Sulfate induced colitis	439:476	In an effort to counteract these characteristic perturbations, we used stem cells and/or a probiotic therapy in a murine model of Dextran Sodium Sulfate induced colitis to examine both their efficacy in ameliorating disease and impact on niche-specific microbial communities of the lower GI tract.
25144681	0	37	theme	DSS-induced	16:26	arg1	colitis					35:41	DSS-induced murine colitis	16:41	DSS-induced murine colitis	16:41	Amelioration of DSS-induced murine colitis by VSL#3 supplementation is primarily associated with changes in ileal microbiota composition.
25144681	6	38	from	composition	1311:1321	arg1	niches					1336:1341	all three niches	1326:1341	all three niches	1326:1341	DSS-treatment perturbed community composition in all three niches with ileal communities exhibiting the greatest change relative to control animals.
25144681	3	39	theme	drinking	670:677	arg1	water					679:683	drinking water	670:683	drinking water	670:683	Colitis was induced in C57BL/6 mice by administering 3% DSS in drinking water for 10 days prior to administering one of three treatment plans: daily probiotic (VSL#3) supplementation for 3 days, a single tail vein injection of 1x10 (6) murine mesenchymal stem cells, or both.
25144681	0	40	theme	colitis	35:41	arg1	Amelioration					0:11	Amelioration	0:11	Amelioration of DSS-induced murine colitis by VSL#3 supplementation	0:66	Amelioration of DSS-induced murine colitis by VSL#3 supplementation is primarily associated with changes in ileal microbiota composition.
25144681	7	41	theme	GI	1527:1528	arg1	tract					1530:1534	the lower GI tract	1517:1534	the lower GI tract	1517:1534	Each treatment group exhibited treatment-specific alterations in microbiota composition in the lower GI tract, though disease scores were only improved in VSL#3-treated animals.
25144681	5	42	theme	Microbiota	974:983	arg1	profiling					985:993	Microbiota profiling	974:993	Microbiota profiling	974:993	Microbiota profiling revealed distinct bacterial community compositions in the ileum, cecum and colon of control untreated animals, all of which were predicted in silico to be enriched for a number of discrete KEGG pathways, indicating compositional and functional niche specificity in healthy animals.
25144681	2	43	theme	stem	380:383	arg1	cells					385:389	stem cells	380:389	stem cells	380:389	In an effort to counteract these characteristic perturbations, we used stem cells and/or a probiotic therapy in a murine model of Dextran Sodium Sulfate induced colitis to examine both their efficacy in ameliorating disease and impact on niche-specific microbial communities of the lower GI tract.
25144681	0	44	theme	VSL	46:48	arg1	supplementation					52:66	VSL#3 supplementation	46:66	VSL#3 supplementation	46:66	Amelioration of DSS-induced murine colitis by VSL#3 supplementation is primarily associated with changes in ileal microbiota composition.
25144681	6	45	theme	control	1409:1415	arg1	animals					1417:1423	control animals	1409:1423	control animals	1409:1423	DSS-treatment perturbed community composition in all three niches with ileal communities exhibiting the greatest change relative to control animals.
25144681	2	46	theme	probiotic	400:408	arg1	therapy					410:416	a probiotic therapy	398:416	a probiotic therapy	398:416	In an effort to counteract these characteristic perturbations, we used stem cells and/or a probiotic therapy in a murine model of Dextran Sodium Sulfate induced colitis to examine both their efficacy in ameliorating disease and impact on niche-specific microbial communities of the lower GI tract.
25144681	5	47	theme	discrete	1175:1182	arg1	pathways					1189:1196	discrete KEGG pathways	1175:1196	discrete KEGG pathways	1175:1196	Microbiota profiling revealed distinct bacterial community compositions in the ileum, cecum and colon of control untreated animals, all of which were predicted in silico to be enriched for a number of discrete KEGG pathways, indicating compositional and functional niche specificity in healthy animals.
25144681	0	48	theme	#	49:49	arg1	supplementation					52:66	VSL#3 supplementation	46:66	VSL#3 supplementation	46:66	Amelioration of DSS-induced murine colitis by VSL#3 supplementation is primarily associated with changes in ileal microbiota composition.
25144681	1	49	theme	gastrointestinal	270:285	arg1	dysbiosis					298:306	gastrointestinal microbiota dysbiosis	270:306	gastrointestinal microbiota dysbiosis	270:306	Inflammatory bowel diseases encompass gastrointestinal illnesses typified by chronic inflammation, loss of epithelial integrity and gastrointestinal microbiota dysbiosis.
25144681	7	50	theme	treatment-specific	1457:1474	arg1	alterations					1476:1486	treatment-specific alterations	1457:1486	treatment-specific alterations in microbiota composition in the lower GI tract	1457:1534	Each treatment group exhibited treatment-specific alterations in microbiota composition in the lower GI tract, though disease scores were only improved in VSL#3-treated animals.
25144681	8	51	theme	ileal	1608:1612	arg1	microbiota					1614:1623	The ileal microbiota	1604:1623	The ileal microbiota	1604:1623	The ileal microbiota were most profoundly altered in composition in this group of animals and characterized by significant Enterobacteriaceae enrichment compared with colitic mice (P<0.05).
25144681	8	52	theme	animals	1686:1692	arg1	animals					1686:1692	animals	1686:1692	animals	1686:1692	The ileal microbiota were most profoundly altered in composition in this group of animals and characterized by significant Enterobacteriaceae enrichment compared with colitic mice (P<0.05).
25144681	8	52	theme	animals	1686:1692	arg1	group					1677:1681	this group	1672:1681	this group of animals	1672:1692	The ileal microbiota were most profoundly altered in composition in this group of animals and characterized by significant Enterobacteriaceae enrichment compared with colitic mice (P<0.05).
25144681	1	53	theme	gastrointestinal	176:191	arg1	illnesses					193:201	gastrointestinal illnesses	176:201	gastrointestinal illnesses typified by chronic inflammation, loss of epithelial integrity and gastrointestinal microbiota dysbiosis	176:306	Inflammatory bowel diseases encompass gastrointestinal illnesses typified by chronic inflammation, loss of epithelial integrity and gastrointestinal microbiota dysbiosis.
25144681	5	54	theme	animals	1097:1103	arg1	colon					1070:1074	colon	1070:1074	colon	1070:1074	Microbiota profiling revealed distinct bacterial community compositions in the ileum, cecum and colon of control untreated animals, all of which were predicted in silico to be enriched for a number of discrete KEGG pathways, indicating compositional and functional niche specificity in healthy animals.
25144681	5	54	theme	animals	1097:1103	arg1	cecum					1060:1064	cecum	1060:1064	cecum	1060:1064	Microbiota profiling revealed distinct bacterial community compositions in the ileum, cecum and colon of control untreated animals, all of which were predicted in silico to be enriched for a number of discrete KEGG pathways, indicating compositional and functional niche specificity in healthy animals.
25144681	5	54	theme	animals	1097:1103	arg1	ileum					1053:1057	ileum	1053:1057	ileum	1053:1057	Microbiota profiling revealed distinct bacterial community compositions in the ileum, cecum and colon of control untreated animals, all of which were predicted in silico to be enriched for a number of discrete KEGG pathways, indicating compositional and functional niche specificity in healthy animals.
25144681	0	55	theme	microbiota	114:123	arg1	composition					125:135	ileal microbiota composition	108:135	ileal microbiota composition	108:135	Amelioration of DSS-induced murine colitis by VSL#3 supplementation is primarily associated with changes in ileal microbiota composition.
25144681	8	56	theme	significant	1715:1725	arg1	Enterobacteriaceae					1727:1744	significant Enterobacteriaceae	1715:1744	significant Enterobacteriaceae enrichment compared with colitic mice (P<0.05)	1715:1791	The ileal microbiota were most profoundly altered in composition in this group of animals and characterized by significant Enterobacteriaceae enrichment compared with colitic mice (P<0.05).
25144681	1	57	theme	chronic	215:221	arg1	inflammation					223:234	chronic inflammation	215:234	chronic inflammation	215:234	Inflammatory bowel diseases encompass gastrointestinal illnesses typified by chronic inflammation, loss of epithelial integrity and gastrointestinal microbiota dysbiosis.
25144681	3	58	theme	daily	750:754	arg1	supplementation					774:788	daily probiotic (VSL#3) supplementation	750:788	daily probiotic (VSL#3) supplementation for 3 days	750:799	Colitis was induced in C57BL/6 mice by administering 3% DSS in drinking water for 10 days prior to administering one of three treatment plans: daily probiotic (VSL#3) supplementation for 3 days, a single tail vein injection of 1x10 (6) murine mesenchymal stem cells, or both.
25144681	8	59	theme	enrichment	1746:1755	arg1	Enterobacteriaceae					1727:1744	significant Enterobacteriaceae	1715:1744	significant Enterobacteriaceae enrichment compared with colitic mice (P<0.05)	1715:1791	The ileal microbiota were most profoundly altered in composition in this group of animals and characterized by significant Enterobacteriaceae enrichment compared with colitic mice (P<0.05).
25144681	7	60	from	alterations	1476:1486	arg1	composition					1502:1512	microbiota composition	1491:1512	microbiota composition in the lower GI tract	1491:1534	Each treatment group exhibited treatment-specific alterations in microbiota composition in the lower GI tract, though disease scores were only improved in VSL#3-treated animals.
25144681	4	61	theme	cecal	890:894	arg1	sections					908:915	Ileal, cecal and colonic sections	883:915	Ileal, cecal and colonic sections	883:915	Ileal, cecal and colonic sections were collected for microbiota and histological analyses.
25144681	7	62	theme	3-treated	1585:1593	arg1	animals					1595:1601	VSL#3-treated animals	1581:1601	VSL#3-treated animals	1581:1601	Each treatment group exhibited treatment-specific alterations in microbiota composition in the lower GI tract, though disease scores were only improved in VSL#3-treated animals.
25144681	5	63	theme	bacterial	1013:1021	arg1	compositions					1033:1044	distinct bacterial community compositions	1004:1044	distinct bacterial community compositions	1004:1044	Microbiota profiling revealed distinct bacterial community compositions in the ileum, cecum and colon of control untreated animals, all of which were predicted in silico to be enriched for a number of discrete KEGG pathways, indicating compositional and functional niche specificity in healthy animals.
25144681	7	64	theme	VSL	1581:1583	arg1	animals					1595:1601	VSL#3-treated animals	1581:1601	VSL#3-treated animals	1581:1601	Each treatment group exhibited treatment-specific alterations in microbiota composition in the lower GI tract, though disease scores were only improved in VSL#3-treated animals.
25144681	2	65	theme	GI	597:598	arg1	tract					600:604	the lower GI tract	587:604	the lower GI tract	587:604	In an effort to counteract these characteristic perturbations, we used stem cells and/or a probiotic therapy in a murine model of Dextran Sodium Sulfate induced colitis to examine both their efficacy in ameliorating disease and impact on niche-specific microbial communities of the lower GI tract.
25144681	5	66	theme	niche	1239:1243	arg1	specificity					1245:1255	compositional and functional niche specificity	1210:1255	compositional and functional niche specificity	1210:1255	Microbiota profiling revealed distinct bacterial community compositions in the ileum, cecum and colon of control untreated animals, all of which were predicted in silico to be enriched for a number of discrete KEGG pathways, indicating compositional and functional niche specificity in healthy animals.
25144681	1	67	theme	Inflammatory	138:149	arg1	diseases					157:164	Inflammatory bowel diseases	138:164	Inflammatory bowel diseases	138:164	Inflammatory bowel diseases encompass gastrointestinal illnesses typified by chronic inflammation, loss of epithelial integrity and gastrointestinal microbiota dysbiosis.
25144681	5	68	theme	pathways	1189:1196	arg1	number					1165:1170	a number	1163:1170	a number of discrete KEGG pathways	1163:1196	Microbiota profiling revealed distinct bacterial community compositions in the ileum, cecum and colon of control untreated animals, all of which were predicted in silico to be enriched for a number of discrete KEGG pathways, indicating compositional and functional niche specificity in healthy animals.
25144681	6	69	theme	ileal	1348:1352	arg1	communities					1354:1364	ileal communities	1348:1364	ileal communities exhibiting the greatest change relative to control animals	1348:1423	DSS-treatment perturbed community composition in all three niches with ileal communities exhibiting the greatest change relative to control animals.
25144681	5	70	theme	untreated	1087:1095	arg1	animals					1097:1103	control untreated animals	1079:1103	control untreated animals	1079:1103	Microbiota profiling revealed distinct bacterial community compositions in the ileum, cecum and colon of control untreated animals, all of which were predicted in silico to be enriched for a number of discrete KEGG pathways, indicating compositional and functional niche specificity in healthy animals.
25144681	3	71	theme	murine	843:848	arg1	cells					867:871	1x10 (6) murine mesenchymal stem cells	834:871	1x10 (6) murine mesenchymal stem cells	834:871	Colitis was induced in C57BL/6 mice by administering 3% DSS in drinking water for 10 days prior to administering one of three treatment plans: daily probiotic (VSL#3) supplementation for 3 days, a single tail vein injection of 1x10 (6) murine mesenchymal stem cells, or both.
25144681	6	72	theme	community	1301:1309	arg1	composition					1311:1321	community composition	1301:1321	community composition in all three niches with ileal communities exhibiting the greatest change relative to control animals	1301:1423	DSS-treatment perturbed community composition in all three niches with ileal communities exhibiting the greatest change relative to control animals.
25144681	3	73	theme	tail	811:814	arg1	injection					821:829	a single tail vein injection	802:829	a single tail vein injection of 1x10 (6) murine mesenchymal stem cells	802:871	Colitis was induced in C57BL/6 mice by administering 3% DSS in drinking water for 10 days prior to administering one of three treatment plans: daily probiotic (VSL#3) supplementation for 3 days, a single tail vein injection of 1x10 (6) murine mesenchymal stem cells, or both.
25144681	7	74	theme	disease	1544:1550	arg1	scores					1552:1557	disease scores	1544:1557	disease scores	1544:1557	Each treatment group exhibited treatment-specific alterations in microbiota composition in the lower GI tract, though disease scores were only improved in VSL#3-treated animals.
25144681	2	75	theme	niche-specific	547:560	arg1	communities					572:582	niche-specific microbial communities	547:582	niche-specific microbial communities	547:582	In an effort to counteract these characteristic perturbations, we used stem cells and/or a probiotic therapy in a murine model of Dextran Sodium Sulfate induced colitis to examine both their efficacy in ameliorating disease and impact on niche-specific microbial communities of the lower GI tract.
25144681	2	76	from	efficacy	500:507	arg1	communities					572:582	niche-specific microbial communities	547:582	niche-specific microbial communities	547:582	In an effort to counteract these characteristic perturbations, we used stem cells and/or a probiotic therapy in a murine model of Dextran Sodium Sulfate induced colitis to examine both their efficacy in ameliorating disease and impact on niche-specific microbial communities of the lower GI tract.
25144681	3	77	theme	stem	862:865	arg1	cells					867:871	1x10 (6) murine mesenchymal stem cells	834:871	1x10 (6) murine mesenchymal stem cells	834:871	Colitis was induced in C57BL/6 mice by administering 3% DSS in drinking water for 10 days prior to administering one of three treatment plans: daily probiotic (VSL#3) supplementation for 3 days, a single tail vein injection of 1x10 (6) murine mesenchymal stem cells, or both.
25144681	2	78	used	used	375:378	arg2	we					372:373	we	372:373	we	372:373	In an effort to counteract these characteristic perturbations, we used stem cells and/or a probiotic therapy in a murine model of Dextran Sodium Sulfate induced colitis to examine both their efficacy in ameliorating disease and impact on niche-specific microbial communities of the lower GI tract.
25144681	0	79	theme	murine	28:33	arg1	colitis					35:41	DSS-induced murine colitis	16:41	DSS-induced murine colitis	16:41	Amelioration of DSS-induced murine colitis by VSL#3 supplementation is primarily associated with changes in ileal microbiota composition.
25144681	2	80	theme	Sulfate	454:460	arg1	model					430:434	a murine model	421:434	a murine model of Dextran Sodium Sulfate induced colitis	421:476	In an effort to counteract these characteristic perturbations, we used stem cells and/or a probiotic therapy in a murine model of Dextran Sodium Sulfate induced colitis to examine both their efficacy in ameliorating disease and impact on niche-specific microbial communities of the lower GI tract.
25144681	2	81	theme	Dextran	439:445	arg1	Sulfate					454:460	Dextran Sodium Sulfate	439:460	Dextran Sodium Sulfate induced colitis	439:476	In an effort to counteract these characteristic perturbations, we used stem cells and/or a probiotic therapy in a murine model of Dextran Sodium Sulfate induced colitis to examine both their efficacy in ameliorating disease and impact on niche-specific microbial communities of the lower GI tract.
25144681	6	82	theme	relative	1397:1404	arg1	change					1390:1395	the greatest change	1377:1395	the greatest change relative to control animals	1377:1423	DSS-treatment perturbed community composition in all three niches with ileal communities exhibiting the greatest change relative to control animals.
25144681	3	83	theme	%	661:661	arg1	DSS					663:665	3% DSS	660:665	3% DSS in drinking water for 10 days prior to administering one of three treatment plans	660:747	Colitis was induced in C57BL/6 mice by administering 3% DSS in drinking water for 10 days prior to administering one of three treatment plans: daily probiotic (VSL#3) supplementation for 3 days, a single tail vein injection of 1x10 (6) murine mesenchymal stem cells, or both.
25144681	1	84	theme	integrity	256:264	arg1	inflammation					223:234	chronic inflammation	215:234	chronic inflammation	215:234	Inflammatory bowel diseases encompass gastrointestinal illnesses typified by chronic inflammation, loss of epithelial integrity and gastrointestinal microbiota dysbiosis.
25144681	1	84	theme	integrity	256:264	arg1	dysbiosis					298:306	gastrointestinal microbiota dysbiosis	270:306	gastrointestinal microbiota dysbiosis	270:306	Inflammatory bowel diseases encompass gastrointestinal illnesses typified by chronic inflammation, loss of epithelial integrity and gastrointestinal microbiota dysbiosis.
25144681	1	84	theme	integrity	256:264	arg1	loss					237:240	loss	237:240	loss of epithelial integrity	237:264	Inflammatory bowel diseases encompass gastrointestinal illnesses typified by chronic inflammation, loss of epithelial integrity and gastrointestinal microbiota dysbiosis.
25144681	6	85	theme	greatest	1381:1388	arg1	change					1390:1395	the greatest change	1377:1395	the greatest change relative to control animals	1377:1423	DSS-treatment perturbed community composition in all three niches with ileal communities exhibiting the greatest change relative to control animals.
25144681	4	86	theme	histological	951:962	arg1	analyses					964:971	histological analyses	951:971	histological analyses	951:971	Ileal, cecal and colonic sections were collected for microbiota and histological analyses.
25144681	7	87	theme	lower	1521:1525	arg1	tract					1530:1534	the lower GI tract	1517:1534	the lower GI tract	1517:1534	Each treatment group exhibited treatment-specific alterations in microbiota composition in the lower GI tract, though disease scores were only improved in VSL#3-treated animals.
25144681	1	88	theme	epithelial	245:254	arg1	integrity					256:264	epithelial integrity	245:264	epithelial integrity	245:264	Inflammatory bowel diseases encompass gastrointestinal illnesses typified by chronic inflammation, loss of epithelial integrity and gastrointestinal microbiota dysbiosis.
25144681	7	89	from	composition	1502:1512	arg1	tract					1530:1534	the lower GI tract	1517:1534	the lower GI tract	1517:1534	Each treatment group exhibited treatment-specific alterations in microbiota composition in the lower GI tract, though disease scores were only improved in VSL#3-treated animals.
25144681	5	90	theme	KEGG	1184:1187	arg1	pathways					1189:1196	discrete KEGG pathways	1175:1196	discrete KEGG pathways	1175:1196	Microbiota profiling revealed distinct bacterial community compositions in the ileum, cecum and colon of control untreated animals, all of which were predicted in silico to be enriched for a number of discrete KEGG pathways, indicating compositional and functional niche specificity in healthy animals.
23765695	4	0	theme	PCSM-Nafion	562:572	arg1	composites					588:597	PCSM-Nafion and PCSM-PTFE composites	562:597	PCSM-Nafion and PCSM-PTFE composites	562:597	Then, PCSM-Nafion and PCSM-PTFE composites were assembled with hydrated PCSM and implanted subcutaneously in rats.
23765695	8	1	theme	collagen	1004:1011	arg1	deposition					1013:1022	higher collagen deposition	997:1022	higher collagen deposition	997:1022	PCSM-PTFE exerted higher collagen deposition by area ratio, both within and outside, compared with that of PCSM-Nafion.
23765695	1	2	theme	tissue	147:152	arg1	engineering					154:164	tissue engineering	147:164	tissue engineering	147:164	Chitosan (CS) is widely used as a scaffold material in tissue engineering.
23765695	2	3	theme	FC	318:319	arg1	density					322:328	fibrous capsule (FC) density	301:328	fibrous capsule (FC) density	301:328	The objective of this study was to test whether porous chitosan membrane (PCSM) coating for Nafion used in implantable sensor reduced fibrous capsule (FC) density and promoted superior vascularization compared with PCSM coating for polytetrafluoroethylene (PTFE).
23765695	8	4	theme	higher	997:1002	arg1	deposition					1013:1022	higher collagen deposition	997:1022	higher collagen deposition	997:1022	PCSM-PTFE exerted higher collagen deposition by area ratio, both within and outside, compared with that of PCSM-Nafion.
23765695	7	5	theme	presumed	881:888	arg1	effects					890:896	presumed effects	881:896	presumed effects of porous coatings	881:915	Histological assessments indicated that both composites achieved presumed effects of porous coatings on decreasing collagen deposition and promoting angiogenesis.
23765695	4	6	theme	PCSM-PTFE	578:586	arg1	composites					588:597	PCSM-Nafion and PCSM-PTFE composites	562:597	PCSM-Nafion and PCSM-PTFE composites	562:597	Then, PCSM-Nafion and PCSM-PTFE composites were assembled with hydrated PCSM and implanted subcutaneously in rats.
23765695	4	7	theme	hydrated	619:626	arg1	PCSM					628:631	hydrated PCSM	619:631	hydrated PCSM	619:631	Then, PCSM-Nafion and PCSM-PTFE composites were assembled with hydrated PCSM and implanted subcutaneously in rats.
23765695	1	8	from	material	135:142	arg1	engineering					154:164	tissue engineering	147:164	tissue engineering	147:164	Chitosan (CS) is widely used as a scaffold material in tissue engineering.
23765695	8	9	theme	area	1027:1030	arg1	ratio					1032:1036	area ratio	1027:1036	area ratio	1027:1036	PCSM-PTFE exerted higher collagen deposition by area ratio, both within and outside, compared with that of PCSM-Nafion.
23765695	2	10	theme	superior	343:350	arg1	vascularization					352:366	superior vascularization	343:366	superior vascularization	343:366	The objective of this study was to test whether porous chitosan membrane (PCSM) coating for Nafion used in implantable sensor reduced fibrous capsule (FC) density and promoted superior vascularization compared with PCSM coating for polytetrafluoroethylene (PTFE).
23765695	0	11	theme	Biocompatibility	0:15	arg1	assessment					17:26	Biocompatibility assessment	0:26	Biocompatibility assessment of porous chitosan-Nafion and chitosan-PTFE composites in vivo.	0:90	Biocompatibility assessment of porous chitosan-Nafion and chitosan-PTFE composites in vivo.
23765695	7	12	theme	collagen	931:938	arg1	deposition					940:949	collagen deposition	931:949	collagen deposition	931:949	Histological assessments indicated that both composites achieved presumed effects of porous coatings on decreasing collagen deposition and promoting angiogenesis.
23765695	2	13	theme	implantable	274:284	arg1	sensor					286:291	implantable sensor	274:291	implantable sensor	274:291	The objective of this study was to test whether porous chitosan membrane (PCSM) coating for Nafion used in implantable sensor reduced fibrous capsule (FC) density and promoted superior vascularization compared with PCSM coating for polytetrafluoroethylene (PTFE).
23765695	7	14	theme	Histological	816:827	arg1	assessments					829:839	Histological assessments	816:839	Histological assessments	816:839	Histological assessments indicated that both composites achieved presumed effects of porous coatings on decreasing collagen deposition and promoting angiogenesis.
23765695	0	15	theme	porous	31:36	arg1	composites					72:81	porous chitosan-Nafion and chitosan-PTFE composites	31:81	porous chitosan-Nafion and chitosan-PTFE composites in vivo	31:89	Biocompatibility assessment of porous chitosan-Nafion and chitosan-PTFE composites in vivo.
23765695	1	16	used	used	116:119	arg2	CS					102:103	CS	102:103	CS	102:103	Chitosan (CS) is widely used as a scaffold material in tissue engineering.
23765695	1	16	used	used	116:119	arg2	Chitosan					92:99	Chitosan	92:99	Chitosan (CS)	92:104	Chitosan (CS) is widely used as a scaffold material in tissue engineering.
23765695	1	16	used	used	116:119	arg2	material					135:142	a scaffold material	124:142	a scaffold material in tissue engineering	124:164	Chitosan (CS) is widely used as a scaffold material in tissue engineering.
23765695	5	17	with	comparison	714:723	arg1	PTFE					741:744	PTFE	741:744	PTFE	741:744	The histological analysis was performed in comparison with Nafion and PTFE.
23765695	5	17	with	comparison	714:723	arg1	Nafion					730:735	Nafion	730:735	Nafion	730:735	The histological analysis was performed in comparison with Nafion and PTFE.
23765695	0	18	dep	in	83:84	arg1	vivo					86:89	vivo	86:89	vivo	86:89	Biocompatibility assessment of porous chitosan-Nafion and chitosan-PTFE composites in vivo.
23765695	3	19	theme	silica	506:511	arg1	gel					513:515	silica gel	506:515	silica gel	506:515	PCSM was fabricated with solvent casting/particulate leaching method using silica gel as porogen and characterized in vitro.
23765695	3	20	theme	leaching	484:491	arg1	method					493:498	solvent casting/particulate leaching method	456:498	solvent casting/particulate leaching method using silica gel as porogen	456:526	PCSM was fabricated with solvent casting/particulate leaching method using silica gel as porogen and characterized in vitro.
23765695	2	21	theme	porous	215:220	arg1	coating					247:253	porous chitosan membrane (PCSM) coating	215:253	porous chitosan membrane (PCSM) coating for Nafion used in implantable sensor	215:291	The objective of this study was to test whether porous chitosan membrane (PCSM) coating for Nafion used in implantable sensor reduced fibrous capsule (FC) density and promoted superior vascularization compared with PCSM coating for polytetrafluoroethylene (PTFE).
23765695	0	22	theme	chitosan-Nafion	38:52	arg1	composites					72:81	porous chitosan-Nafion and chitosan-PTFE composites	31:81	porous chitosan-Nafion and chitosan-PTFE composites in vivo	31:89	Biocompatibility assessment of porous chitosan-Nafion and chitosan-PTFE composites in vivo.
23765695	0	23	theme	composites	72:81	arg1	assessment					17:26	Biocompatibility assessment	0:26	Biocompatibility assessment of porous chitosan-Nafion and chitosan-PTFE composites in vivo.	0:90	Biocompatibility assessment of porous chitosan-Nafion and chitosan-PTFE composites in vivo.
23765695	3	24	theme	casting/particulate	464:482	arg1	method					493:498	solvent casting/particulate leaching method	456:498	solvent casting/particulate leaching method using silica gel as porogen	456:526	PCSM was fabricated with solvent casting/particulate leaching method using silica gel as porogen and characterized in vitro.
23765695	0	25	theme	chitosan-PTFE	58:70	arg1	composites					72:81	porous chitosan-Nafion and chitosan-PTFE composites	31:81	porous chitosan-Nafion and chitosan-PTFE composites in vivo	31:89	Biocompatibility assessment of porous chitosan-Nafion and chitosan-PTFE composites in vivo.
23765695	5	26	theme	histological	675:686	arg1	analysis					688:695	The histological analysis	671:695	The histological analysis	671:695	The histological analysis was performed in comparison with Nafion and PTFE.
23765695	2	27	theme	capsule	309:315	arg1	density					322:328	fibrous capsule (FC) density	301:328	fibrous capsule (FC) density	301:328	The objective of this study was to test whether porous chitosan membrane (PCSM) coating for Nafion used in implantable sensor reduced fibrous capsule (FC) density and promoted superior vascularization compared with PCSM coating for polytetrafluoroethylene (PTFE).
23765695	8	28	dep	exerted	989:995	arg1	outside					1055:1061	outside	1055:1061	outside	1055:1061	PCSM-PTFE exerted higher collagen deposition by area ratio, both within and outside, compared with that of PCSM-Nafion.
23765695	8	28	dep	exerted	989:995	arg1	within					1044:1049	within	1044:1049	within	1044:1049	PCSM-PTFE exerted higher collagen deposition by area ratio, both within and outside, compared with that of PCSM-Nafion.
23765695	2	29	theme	study	189:193	arg1	objective					171:179	The objective	167:179	The objective of this study	167:193	The objective of this study was to test whether porous chitosan membrane (PCSM) coating for Nafion used in implantable sensor reduced fibrous capsule (FC) density and promoted superior vascularization compared with PCSM coating for polytetrafluoroethylene (PTFE).
23765695	2	30	theme	PCSM	241:244	arg1	coating					247:253	porous chitosan membrane (PCSM) coating	215:253	porous chitosan membrane (PCSM) coating for Nafion used in implantable sensor	215:291	The objective of this study was to test whether porous chitosan membrane (PCSM) coating for Nafion used in implantable sensor reduced fibrous capsule (FC) density and promoted superior vascularization compared with PCSM coating for polytetrafluoroethylene (PTFE).
23765695	2	31	theme	fibrous	301:307	arg1	density					322:328	fibrous capsule (FC) density	301:328	fibrous capsule (FC) density	301:328	The objective of this study was to test whether porous chitosan membrane (PCSM) coating for Nafion used in implantable sensor reduced fibrous capsule (FC) density and promoted superior vascularization compared with PCSM coating for polytetrafluoroethylene (PTFE).
23765695	3	32	theme	solvent	456:462	arg1	method					493:498	solvent casting/particulate leaching method	456:498	solvent casting/particulate leaching method using silica gel as porogen	456:526	PCSM was fabricated with solvent casting/particulate leaching method using silica gel as porogen and characterized in vitro.
23765695	7	33	theme	porous	901:906	arg1	coatings					908:915	porous coatings	901:915	porous coatings	901:915	Histological assessments indicated that both composites achieved presumed effects of porous coatings on decreasing collagen deposition and promoting angiogenesis.
23765695	2	34	theme	membrane	231:238	arg1	coating					247:253	porous chitosan membrane (PCSM) coating	215:253	porous chitosan membrane (PCSM) coating for Nafion used in implantable sensor	215:291	The objective of this study was to test whether porous chitosan membrane (PCSM) coating for Nafion used in implantable sensor reduced fibrous capsule (FC) density and promoted superior vascularization compared with PCSM coating for polytetrafluoroethylene (PTFE).
23765695	1	35	theme	scaffold	126:133	arg1	material					135:142	a scaffold material	124:142	a scaffold material in tissue engineering	124:164	Chitosan (CS) is widely used as a scaffold material in tissue engineering.
23765695	1	35	theme	scaffold	126:133	arg1	Chitosan					92:99	Chitosan	92:99	Chitosan (CS)	92:104	Chitosan (CS) is widely used as a scaffold material in tissue engineering.
23765695	7	36	theme	coatings	908:915	arg1	effects					890:896	presumed effects	881:896	presumed effects of porous coatings	881:915	Histological assessments indicated that both composites achieved presumed effects of porous coatings on decreasing collagen deposition and promoting angiogenesis.
23765695	0	37	theme	in	83:84	arg1	composites					72:81	porous chitosan-Nafion and chitosan-PTFE composites	31:81	porous chitosan-Nafion and chitosan-PTFE composites in vivo	31:89	Biocompatibility assessment of porous chitosan-Nafion and chitosan-PTFE composites in vivo.
23765695	2	38	theme	chitosan	222:229	arg1	coating					247:253	porous chitosan membrane (PCSM) coating	215:253	porous chitosan membrane (PCSM) coating for Nafion used in implantable sensor	215:291	The objective of this study was to test whether porous chitosan membrane (PCSM) coating for Nafion used in implantable sensor reduced fibrous capsule (FC) density and promoted superior vascularization compared with PCSM coating for polytetrafluoroethylene (PTFE).
28962775	1	0	dep	burst	268:272	arg1	releases					274:281	releases	274:281	releases associated with the pure form of the hydrogels	274:328	A Series of in-situ alginate-brushite (Alg-Bru) hydrogel composites were fabricated to optimize release profile of ibuprofen (Ibu) and to avoid burst releases associated with the pure form of the hydrogels.
28962775	1	1	theme	in-situ	136:142	arg1	composites					181:190	in-situ alginate-brushite (Alg-Bru) hydrogel composites	136:190	in-situ alginate-brushite (Alg-Bru) hydrogel composites	136:190	A Series of in-situ alginate-brushite (Alg-Bru) hydrogel composites were fabricated to optimize release profile of ibuprofen (Ibu) and to avoid burst releases associated with the pure form of the hydrogels.
28962775	1	2	theme	ibuprofen	239:247	arg1	profile					228:234	release profile	220:234	release profile of ibuprofen (Ibu)	220:253	A Series of in-situ alginate-brushite (Alg-Bru) hydrogel composites were fabricated to optimize release profile of ibuprofen (Ibu) and to avoid burst releases associated with the pure form of the hydrogels.
28962775	3	3	theme	mechanical	636:645	arg1	DMA					657:659	DMA	657:659	DMA	657:659	The beads with different formulations were subject to various characterization tests such as X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), dynamic mechanical analysis (DMA), and swelling.
28962775	3	3	theme	mechanical	636:645	arg1	analysis					647:654	dynamic mechanical analysis	628:654	dynamic mechanical analysis (DMA)	628:660	The beads with different formulations were subject to various characterization tests such as X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), dynamic mechanical analysis (DMA), and swelling.
28962775	1	4	theme	alginate-brushite	144:160	arg1	composites					181:190	in-situ alginate-brushite (Alg-Bru) hydrogel composites	136:190	in-situ alginate-brushite (Alg-Bru) hydrogel composites	136:190	A Series of in-situ alginate-brushite (Alg-Bru) hydrogel composites were fabricated to optimize release profile of ibuprofen (Ibu) and to avoid burst releases associated with the pure form of the hydrogels.
28962775	4	5	from	impacts	780:786	arg1	parameters					848:857	these parameters	842:857	these parameters	842:857	In addition, the entrapment Efficiency (%EE) and drug release profile were obtained to investigate the impacts of initial concentration of Alg and content of Bru on these parameters.
28962775	4	6	theme	concentration	799:811	arg1	impacts					780:786	the impacts	776:786	the impacts of initial concentration of Alg and content of Bru on these parameters	776:857	In addition, the entrapment Efficiency (%EE) and drug release profile were obtained to investigate the impacts of initial concentration of Alg and content of Bru on these parameters.
28962775	7	7	theme	gradual	1242:1248	arg1	manner					1250:1255	a more gradual manner	1235:1255	a more gradual manner	1235:1255	Although the presence of Bru crystals did not improve%EE, they optimized the release profile in a more gradual manner.
28962775	3	8	theme	X-ray	519:523	arg1	XRD					538:540	XRD	538:540	XRD	538:540	The beads with different formulations were subject to various characterization tests such as X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), dynamic mechanical analysis (DMA), and swelling.
28962775	3	8	theme	X-ray	519:523	arg1	diffraction					525:535	X-ray diffraction	519:535	X-ray diffraction (XRD)	519:541	The beads with different formulations were subject to various characterization tests such as X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), dynamic mechanical analysis (DMA), and swelling.
28962775	6	9	theme	Alg	1031:1033	arg1	movement					1067:1074	Alg matrix restricted polymer chain movement	1031:1074	Alg matrix restricted polymer chain movement	1031:1074	Besides, the results showed that the presence of Bru within Alg matrix restricted polymer chain movement, improved mechanical properties, and decreased swelling ratio.
28962775	0	10	theme	potential	79:87	arg1	system					116:121	a potential pH sensitive drug delivery system	77:121	a potential pH sensitive drug delivery system	77:121	New in-situ synthetized hydrogel composite based on alginate and brushite as a potential pH sensitive drug delivery system.
28962775	0	10	theme	potential	79:87	arg1	composite					33:41	hydrogel composite	24:41	hydrogel composite based on alginate and brushite	24:72	New in-situ synthetized hydrogel composite based on alginate and brushite as a potential pH sensitive drug delivery system.
28962775	1	11	theme	hydrogels	320:328	arg1	form					308:311	the pure form	299:311	the pure form of the hydrogels	299:328	A Series of in-situ alginate-brushite (Alg-Bru) hydrogel composites were fabricated to optimize release profile of ibuprofen (Ibu) and to avoid burst releases associated with the pure form of the hydrogels.
28962775	4	12	theme	initial	791:797	arg1	concentration					799:811	initial concentration	791:811	initial concentration of Alg	791:818	In addition, the entrapment Efficiency (%EE) and drug release profile were obtained to investigate the impacts of initial concentration of Alg and content of Bru on these parameters.
28962775	0	13	theme	drug	102:105	arg1	system					116:121	a potential pH sensitive drug delivery system	77:121	a potential pH sensitive drug delivery system	77:121	New in-situ synthetized hydrogel composite based on alginate and brushite as a potential pH sensitive drug delivery system.
28962775	0	13	theme	drug	102:105	arg1	composite					33:41	hydrogel composite	24:41	hydrogel composite based on alginate and brushite	24:72	New in-situ synthetized hydrogel composite based on alginate and brushite as a potential pH sensitive drug delivery system.
28962775	3	14	dep	Fourier	544:550	arg1	transform					552:560	transform	552:560	transform infrared spectroscopy (FTIR)	552:589	The beads with different formulations were subject to various characterization tests such as X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), dynamic mechanical analysis (DMA), and swelling.
28962775	0	15	theme	sensitive	92:100	arg1	system					116:121	a potential pH sensitive drug delivery system	77:121	a potential pH sensitive drug delivery system	77:121	New in-situ synthetized hydrogel composite based on alginate and brushite as a potential pH sensitive drug delivery system.
28962775	0	15	theme	sensitive	92:100	arg1	composite					33:41	hydrogel composite	24:41	hydrogel composite based on alginate and brushite	24:72	New in-situ synthetized hydrogel composite based on alginate and brushite as a potential pH sensitive drug delivery system.
28962775	1	16	dep	releases	274:281	arg1	associated					283:292	associated	283:292	releases associated with the pure form of the hydrogels	274:328	A Series of in-situ alginate-brushite (Alg-Bru) hydrogel composites were fabricated to optimize release profile of ibuprofen (Ibu) and to avoid burst releases associated with the pure form of the hydrogels.
28962775	5	17	dep	FTIR	860:863	arg1	outcomes					873:880	outcomes	873:880	outcomes	873:880	FTIR and XRD outcomes confirmed the successful fabricating of Alg-Bru composite as well as the loading of Ibu.
28962775	6	18	theme	chain	1061:1065	arg1	movement					1067:1074	Alg matrix restricted polymer chain movement	1031:1074	Alg matrix restricted polymer chain movement	1031:1074	Besides, the results showed that the presence of Bru within Alg matrix restricted polymer chain movement, improved mechanical properties, and decreased swelling ratio.
28962775	1	19	theme	Alg-Bru	163:169	arg1	composites					181:190	in-situ alginate-brushite (Alg-Bru) hydrogel composites	136:190	in-situ alginate-brushite (Alg-Bru) hydrogel composites	136:190	A Series of in-situ alginate-brushite (Alg-Bru) hydrogel composites were fabricated to optimize release profile of ibuprofen (Ibu) and to avoid burst releases associated with the pure form of the hydrogels.
28962775	2	20	theme	matrix	418:423	arg1	process					403:409	the crosslinking process	386:409	the crosslinking process of Alg matrix	386:423	The Bru crystals were synthetized and dispersed during the crosslinking process of Alg matrix.
28962775	6	21	theme	polymer	1053:1059	arg1	movement					1067:1074	Alg matrix restricted polymer chain movement	1031:1074	Alg matrix restricted polymer chain movement	1031:1074	Besides, the results showed that the presence of Bru within Alg matrix restricted polymer chain movement, improved mechanical properties, and decreased swelling ratio.
28962775	2	22	theme	Alg	414:416	arg1	matrix					418:423	Alg matrix	414:423	Alg matrix	414:423	The Bru crystals were synthetized and dispersed during the crosslinking process of Alg matrix.
28962775	6	23	theme	restricted	1042:1051	arg1	movement					1067:1074	Alg matrix restricted polymer chain movement	1031:1074	Alg matrix restricted polymer chain movement	1031:1074	Besides, the results showed that the presence of Bru within Alg matrix restricted polymer chain movement, improved mechanical properties, and decreased swelling ratio.
28962775	4	24	theme	entrapment	694:703	arg1	Efficiency					705:714	the entrapment Efficiency	690:714	the entrapment Efficiency (%EE)	690:720	In addition, the entrapment Efficiency (%EE) and drug release profile were obtained to investigate the impacts of initial concentration of Alg and content of Bru on these parameters.
28962775	4	24	theme	entrapment	694:703	arg1	EE					718:719	%EE	717:719	%EE	717:719	In addition, the entrapment Efficiency (%EE) and drug release profile were obtained to investigate the impacts of initial concentration of Alg and content of Bru on these parameters.
28962775	1	25	theme	hydrogel	172:179	arg1	composites					181:190	in-situ alginate-brushite (Alg-Bru) hydrogel composites	136:190	in-situ alginate-brushite (Alg-Bru) hydrogel composites	136:190	A Series of in-situ alginate-brushite (Alg-Bru) hydrogel composites were fabricated to optimize release profile of ibuprofen (Ibu) and to avoid burst releases associated with the pure form of the hydrogels.
28962775	0	26	theme	hydrogel	24:31	arg1	system					116:121	a potential pH sensitive drug delivery system	77:121	a potential pH sensitive drug delivery system	77:121	New in-situ synthetized hydrogel composite based on alginate and brushite as a potential pH sensitive drug delivery system.
28962775	0	26	theme	hydrogel	24:31	arg1	composite					33:41	hydrogel composite	24:41	hydrogel composite based on alginate and brushite	24:72	New in-situ synthetized hydrogel composite based on alginate and brushite as a potential pH sensitive drug delivery system.
28962775	4	27	theme	Bru	835:837	arg1	content					824:830	content	824:830	content of Bru	824:837	In addition, the entrapment Efficiency (%EE) and drug release profile were obtained to investigate the impacts of initial concentration of Alg and content of Bru on these parameters.
28962775	4	27	theme	Bru	835:837	arg1	concentration					799:811	initial concentration	791:811	initial concentration of Alg	791:818	In addition, the entrapment Efficiency (%EE) and drug release profile were obtained to investigate the impacts of initial concentration of Alg and content of Bru on these parameters.
28962775	6	28	theme	matrix	1035:1040	arg1	movement					1067:1074	Alg matrix restricted polymer chain movement	1031:1074	Alg matrix restricted polymer chain movement	1031:1074	Besides, the results showed that the presence of Bru within Alg matrix restricted polymer chain movement, improved mechanical properties, and decreased swelling ratio.
28962775	4	29	theme	release	731:737	arg1	profile					739:745	drug release profile	726:745	drug release profile	726:745	In addition, the entrapment Efficiency (%EE) and drug release profile were obtained to investigate the impacts of initial concentration of Alg and content of Bru on these parameters.
28962775	2	30	theme	crosslinking	390:401	arg1	process					403:409	the crosslinking process	386:409	the crosslinking process of Alg matrix	386:423	The Bru crystals were synthetized and dispersed during the crosslinking process of Alg matrix.
28962775	0	31	theme	delivery	107:114	arg1	system					116:121	a potential pH sensitive drug delivery system	77:121	a potential pH sensitive drug delivery system	77:121	New in-situ synthetized hydrogel composite based on alginate and brushite as a potential pH sensitive drug delivery system.
28962775	0	31	theme	delivery	107:114	arg1	composite					33:41	hydrogel composite	24:41	hydrogel composite based on alginate and brushite	24:72	New in-situ synthetized hydrogel composite based on alginate and brushite as a potential pH sensitive drug delivery system.
28962775	3	32	theme	various	480:486	arg1	swelling					667:674	swelling	667:674	swelling	667:674	The beads with different formulations were subject to various characterization tests such as X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), dynamic mechanical analysis (DMA), and swelling.
28962775	3	32	theme	various	480:486	arg1	microscopy					610:619	scanning electron microscopy	592:619	scanning electron microscopy (SEM)	592:625	The beads with different formulations were subject to various characterization tests such as X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), dynamic mechanical analysis (DMA), and swelling.
28962775	3	32	theme	various	480:486	arg1	tests					505:509	various characterization tests	480:509	various characterization tests such as X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), dynamic mechanical analysis (DMA), and swelling	480:674	The beads with different formulations were subject to various characterization tests such as X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), dynamic mechanical analysis (DMA), and swelling.
28962775	3	32	theme	various	480:486	arg1	analysis					647:654	dynamic mechanical analysis	628:654	dynamic mechanical analysis (DMA)	628:660	The beads with different formulations were subject to various characterization tests such as X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), dynamic mechanical analysis (DMA), and swelling.
28962775	3	32	theme	various	480:486	arg1	Fourier					544:550	Fourier	544:550	Fourier transform infrared spectroscopy (FTIR)	544:589	The beads with different formulations were subject to various characterization tests such as X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), dynamic mechanical analysis (DMA), and swelling.
28962775	3	32	theme	various	480:486	arg1	diffraction					525:535	X-ray diffraction	519:535	X-ray diffraction (XRD)	519:541	The beads with different formulations were subject to various characterization tests such as X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), dynamic mechanical analysis (DMA), and swelling.
28962775	3	33	theme	characterization	488:503	arg1	swelling					667:674	swelling	667:674	swelling	667:674	The beads with different formulations were subject to various characterization tests such as X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), dynamic mechanical analysis (DMA), and swelling.
28962775	3	33	theme	characterization	488:503	arg1	microscopy					610:619	scanning electron microscopy	592:619	scanning electron microscopy (SEM)	592:625	The beads with different formulations were subject to various characterization tests such as X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), dynamic mechanical analysis (DMA), and swelling.
28962775	3	33	theme	characterization	488:503	arg1	tests					505:509	various characterization tests	480:509	various characterization tests such as X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), dynamic mechanical analysis (DMA), and swelling	480:674	The beads with different formulations were subject to various characterization tests such as X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), dynamic mechanical analysis (DMA), and swelling.
28962775	3	33	theme	characterization	488:503	arg1	analysis					647:654	dynamic mechanical analysis	628:654	dynamic mechanical analysis (DMA)	628:660	The beads with different formulations were subject to various characterization tests such as X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), dynamic mechanical analysis (DMA), and swelling.
28962775	3	33	theme	characterization	488:503	arg1	Fourier					544:550	Fourier	544:550	Fourier transform infrared spectroscopy (FTIR)	544:589	The beads with different formulations were subject to various characterization tests such as X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), dynamic mechanical analysis (DMA), and swelling.
28962775	3	33	theme	characterization	488:503	arg1	diffraction					525:535	X-ray diffraction	519:535	X-ray diffraction (XRD)	519:541	The beads with different formulations were subject to various characterization tests such as X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), dynamic mechanical analysis (DMA), and swelling.
28962775	7	34	theme	%	1192:1192	arg1	EE					1193:1194	%EE	1192:1194	%EE	1192:1194	Although the presence of Bru crystals did not improve%EE, they optimized the release profile in a more gradual manner.
28962775	3	35	with	beads	430:434	arg1	formulations					451:462	different formulations	441:462	different formulations	441:462	The beads with different formulations were subject to various characterization tests such as X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), dynamic mechanical analysis (DMA), and swelling.
28962775	3	36	theme	infrared	562:569	arg1	FTIR					585:588	FTIR	585:588	FTIR	585:588	The beads with different formulations were subject to various characterization tests such as X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), dynamic mechanical analysis (DMA), and swelling.
28962775	3	36	theme	infrared	562:569	arg1	spectroscopy					571:582	infrared spectroscopy	562:582	infrared spectroscopy (FTIR)	562:589	The beads with different formulations were subject to various characterization tests such as X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), dynamic mechanical analysis (DMA), and swelling.
28962775	3	37	theme	scanning	592:599	arg1	microscopy					610:619	scanning electron microscopy	592:619	scanning electron microscopy (SEM)	592:625	The beads with different formulations were subject to various characterization tests such as X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), dynamic mechanical analysis (DMA), and swelling.
28962775	3	37	theme	scanning	592:599	arg1	SEM					622:624	SEM	622:624	SEM	622:624	The beads with different formulations were subject to various characterization tests such as X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), dynamic mechanical analysis (DMA), and swelling.
28962775	4	38	theme	drug	726:729	arg1	profile					739:745	drug release profile	726:745	drug release profile	726:745	In addition, the entrapment Efficiency (%EE) and drug release profile were obtained to investigate the impacts of initial concentration of Alg and content of Bru on these parameters.
28962775	5	39	theme	composite	930:938	arg1	fabricating					907:917	the successful fabricating	892:917	the successful fabricating of Alg-Bru composite as well as the loading of Ibu	892:968	FTIR and XRD outcomes confirmed the successful fabricating of Alg-Bru composite as well as the loading of Ibu.
28962775	5	39	theme	composite	930:938	arg1	loading					955:961	the loading	951:961	the successful fabricating of Alg-Bru composite as well as the loading of Ibu	892:968	FTIR and XRD outcomes confirmed the successful fabricating of Alg-Bru composite as well as the loading of Ibu.
28962775	4	40	theme	Alg	816:818	arg1	content					824:830	content	824:830	content of Bru	824:837	In addition, the entrapment Efficiency (%EE) and drug release profile were obtained to investigate the impacts of initial concentration of Alg and content of Bru on these parameters.
28962775	4	40	theme	Alg	816:818	arg1	concentration					799:811	initial concentration	791:811	initial concentration of Alg	791:818	In addition, the entrapment Efficiency (%EE) and drug release profile were obtained to investigate the impacts of initial concentration of Alg and content of Bru on these parameters.
28962775	3	41	theme	electron	601:608	arg1	microscopy					610:619	scanning electron microscopy	592:619	scanning electron microscopy (SEM)	592:625	The beads with different formulations were subject to various characterization tests such as X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), dynamic mechanical analysis (DMA), and swelling.
28962775	3	41	theme	electron	601:608	arg1	SEM					622:624	SEM	622:624	SEM	622:624	The beads with different formulations were subject to various characterization tests such as X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), dynamic mechanical analysis (DMA), and swelling.
28962775	6	42	theme	swelling	1123:1130	arg1	ratio					1132:1136	swelling ratio	1123:1136	swelling ratio	1123:1136	Besides, the results showed that the presence of Bru within Alg matrix restricted polymer chain movement, improved mechanical properties, and decreased swelling ratio.
28962775	6	43	theme	Bru	1020:1022	arg1	presence					1008:1015	the presence	1004:1015	the presence	1004:1015	Besides, the results showed that the presence of Bru within Alg matrix restricted polymer chain movement, improved mechanical properties, and decreased swelling ratio.
28962775	6	44	theme	mechanical	1086:1095	arg1	properties					1097:1106	mechanical properties	1086:1106	mechanical properties	1086:1106	Besides, the results showed that the presence of Bru within Alg matrix restricted polymer chain movement, improved mechanical properties, and decreased swelling ratio.
28962775	4	45	theme	content	824:830	arg1	impacts					780:786	the impacts	776:786	the impacts of initial concentration of Alg and content of Bru on these parameters	776:857	In addition, the entrapment Efficiency (%EE) and drug release profile were obtained to investigate the impacts of initial concentration of Alg and content of Bru on these parameters.
28962775	3	46	theme	dynamic	628:634	arg1	DMA					657:659	DMA	657:659	DMA	657:659	The beads with different formulations were subject to various characterization tests such as X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), dynamic mechanical analysis (DMA), and swelling.
28962775	3	46	theme	dynamic	628:634	arg1	analysis					647:654	dynamic mechanical analysis	628:654	dynamic mechanical analysis (DMA)	628:660	The beads with different formulations were subject to various characterization tests such as X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), dynamic mechanical analysis (DMA), and swelling.
28962775	5	47	theme	successful	896:905	arg1	fabricating					907:917	the successful fabricating	892:917	the successful fabricating of Alg-Bru composite as well as the loading of Ibu	892:968	FTIR and XRD outcomes confirmed the successful fabricating of Alg-Bru composite as well as the loading of Ibu.
28962775	3	48	theme	different	441:449	arg1	formulations					451:462	different formulations	441:462	different formulations	441:462	The beads with different formulations were subject to various characterization tests such as X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), dynamic mechanical analysis (DMA), and swelling.
28962775	4	49	theme	%	717:717	arg1	Efficiency					705:714	the entrapment Efficiency	690:714	the entrapment Efficiency (%EE)	690:720	In addition, the entrapment Efficiency (%EE) and drug release profile were obtained to investigate the impacts of initial concentration of Alg and content of Bru on these parameters.
28962775	4	49	theme	%	717:717	arg1	EE					718:719	%EE	717:719	%EE	717:719	In addition, the entrapment Efficiency (%EE) and drug release profile were obtained to investigate the impacts of initial concentration of Alg and content of Bru on these parameters.
28962775	7	50	theme	crystals	1168:1175	arg1	presence					1152:1159	the presence	1148:1159	the presence of Bru crystals	1148:1175	Although the presence of Bru crystals did not improve%EE, they optimized the release profile in a more gradual manner.
28962775	1	51	theme	release	220:226	arg1	profile					228:234	release profile	220:234	release profile of ibuprofen (Ibu)	220:253	A Series of in-situ alginate-brushite (Alg-Bru) hydrogel composites were fabricated to optimize release profile of ibuprofen (Ibu) and to avoid burst releases associated with the pure form of the hydrogels.
28962775	5	52	theme	Ibu	966:968	arg1	fabricating					907:917	the successful fabricating	892:917	the successful fabricating of Alg-Bru composite as well as the loading of Ibu	892:968	FTIR and XRD outcomes confirmed the successful fabricating of Alg-Bru composite as well as the loading of Ibu.
28962775	5	52	theme	Ibu	966:968	arg1	loading					955:961	the loading	951:961	the successful fabricating of Alg-Bru composite as well as the loading of Ibu	892:968	FTIR and XRD outcomes confirmed the successful fabricating of Alg-Bru composite as well as the loading of Ibu.
28962775	1	53	theme	composites	181:190	arg1	Series					126:131	A Series	124:131	A Series of in-situ alginate-brushite (Alg-Bru) hydrogel composites	124:190	A Series of in-situ alginate-brushite (Alg-Bru) hydrogel composites were fabricated to optimize release profile of ibuprofen (Ibu) and to avoid burst releases associated with the pure form of the hydrogels.
28962775	2	54	theme	Bru	335:337	arg1	crystals					339:346	The Bru crystals	331:346	The Bru crystals	331:346	The Bru crystals were synthetized and dispersed during the crosslinking process of Alg matrix.
28962775	7	55	theme	release	1216:1222	arg1	profile					1224:1230	the release profile	1212:1230	the release profile	1212:1230	Although the presence of Bru crystals did not improve%EE, they optimized the release profile in a more gradual manner.
28962775	1	56	theme	pure	303:306	arg1	form					308:311	the pure form	299:311	the pure form of the hydrogels	299:328	A Series of in-situ alginate-brushite (Alg-Bru) hydrogel composites were fabricated to optimize release profile of ibuprofen (Ibu) and to avoid burst releases associated with the pure form of the hydrogels.
28962775	5	57	theme	Alg-Bru	922:928	arg1	composite					930:938	Alg-Bru composite	922:938	Alg-Bru composite	922:938	FTIR and XRD outcomes confirmed the successful fabricating of Alg-Bru composite as well as the loading of Ibu.
28962775	7	58	theme	Bru	1164:1166	arg1	crystals					1168:1175	Bru crystals	1164:1175	Bru crystals	1164:1175	Although the presence of Bru crystals did not improve%EE, they optimized the release profile in a more gradual manner.
27548134	7	0	theme	injections	1470:1479	arg1	safety					1435:1440	safety	1435:1440	safety	1435:1440	The quantitative information contributes to accumulating scientific evidence to better understand the therapy efficacy and safety of complex Chinese medicine injections.
27548134	7	0	theme	injections	1470:1479	arg1	efficacy					1422:1429	therapy efficacy	1414:1429	therapy efficacy	1414:1429	The quantitative information contributes to accumulating scientific evidence to better understand the therapy efficacy and safety of complex Chinese medicine injections.
27548134	6	1	theme	%	1271:1271	arg1	%					1277:1277	0.18%-0.21%	1267:1277	0.18%-0.21%	1267:1277	The results demonstrate that up to 94.69% (w/w) of this injection product are quantitatively determined, in which small molecules and monosaccharide/sucrose account for 0.18%-0.21%, and 53.49%-58.2%, respectively.
27548134	4	2	theme	gel	903:905	arg1	column					933:938	a gel permeation chromatography column	901:938	a gel permeation chromatography column	901:938	The existence of polysaccharides was also examined on a gel permeation chromatography column.
27548134	3	3	theme	acid	830:833	arg1	hydrolysis					835:844	acid hydrolysis	830:844	acid hydrolysis	830:844	A total of 15 representative small molecular components of SQI were simultaneously determined using ultra-high performance liquid chromatography (UHPLC) coupled with quadrupole tandem time-of-flight mass spectrometry (Q-TOF-MS); saccharide composition of SQI was also quantitatively determined by high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) on an amino column before and after acid hydrolysis.
27548134	3	4	theme	high	698:701	arg1	HPLC					738:741	HPLC	738:741	HPLC	738:741	A total of 15 representative small molecular components of SQI were simultaneously determined using ultra-high performance liquid chromatography (UHPLC) coupled with quadrupole tandem time-of-flight mass spectrometry (Q-TOF-MS); saccharide composition of SQI was also quantitatively determined by high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) on an amino column before and after acid hydrolysis.
27548134	3	4	theme	high	698:701	arg1	chromatography					722:735	high performance liquid chromatography	698:735	high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) on an amino column before and after acid hydrolysis	698:844	A total of 15 representative small molecular components of SQI were simultaneously determined using ultra-high performance liquid chromatography (UHPLC) coupled with quadrupole tandem time-of-flight mass spectrometry (Q-TOF-MS); saccharide composition of SQI was also quantitatively determined by high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) on an amino column before and after acid hydrolysis.
27548134	4	5	theme	polysaccharides	864:878	arg1	existence					851:859	The existence	847:859	The existence of polysaccharides	847:878	The existence of polysaccharides was also examined on a gel permeation chromatography column.
27548134	1	6	theme	poor	175:178	arg1	knowledge					180:188	our poor knowledge	171:188	our poor knowledge of their complex chemical profile	171:222	Quality control of Chinese medicine injections remains a challenge due to our poor knowledge of their complex chemical profile.
27548134	5	7	theme	linearity	983:991	arg1	terms					974:978	terms	974:978	terms of linearity, sensitivity, precision, accuracy and stability	974:1039	The method was well validated in terms of linearity, sensitivity, precision, accuracy and stability, and was successfully applied to analyze 13 SQI samples.
27548134	3	8	theme	performance	703:713	arg1	HPLC					738:741	HPLC	738:741	HPLC	738:741	A total of 15 representative small molecular components of SQI were simultaneously determined using ultra-high performance liquid chromatography (UHPLC) coupled with quadrupole tandem time-of-flight mass spectrometry (Q-TOF-MS); saccharide composition of SQI was also quantitatively determined by high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) on an amino column before and after acid hydrolysis.
27548134	3	8	theme	performance	703:713	arg1	chromatography					722:735	high performance liquid chromatography	698:735	high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) on an amino column before and after acid hydrolysis	698:844	A total of 15 representative small molecular components of SQI were simultaneously determined using ultra-high performance liquid chromatography (UHPLC) coupled with quadrupole tandem time-of-flight mass spectrometry (Q-TOF-MS); saccharide composition of SQI was also quantitatively determined by high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) on an amino column before and after acid hydrolysis.
27548134	7	9	theme	quantitative	1316:1327	arg1	information					1329:1339	The quantitative information	1312:1339	The quantitative information	1312:1339	The quantitative information contributes to accumulating scientific evidence to better understand the therapy efficacy and safety of complex Chinese medicine injections.
27548134	3	10	theme	performance	512:522	arg1	UHPLC					547:551	UHPLC	547:551	UHPLC	547:551	A total of 15 representative small molecular components of SQI were simultaneously determined using ultra-high performance liquid chromatography (UHPLC) coupled with quadrupole tandem time-of-flight mass spectrometry (Q-TOF-MS); saccharide composition of SQI was also quantitatively determined by high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) on an amino column before and after acid hydrolysis.
27548134	3	10	theme	performance	512:522	arg1	chromatography					531:544	ultra-high performance liquid chromatography	501:544	ultra-high performance liquid chromatography (UHPLC) coupled with quadrupole tandem time-of-flight mass spectrometry (Q-TOF-MS)	501:627	A total of 15 representative small molecular components of SQI were simultaneously determined using ultra-high performance liquid chromatography (UHPLC) coupled with quadrupole tandem time-of-flight mass spectrometry (Q-TOF-MS); saccharide composition of SQI was also quantitatively determined by high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) on an amino column before and after acid hydrolysis.
27548134	2	11	theme	best-selling	295:306	arg1	injections					308:317	the best-selling injections	291:317	the best-selling injections	291:317	This study aims to investigate the chemical composition of one of the best-selling injections, Shenqi Fuzheng (SQ) injection (SQI), via a full component quantitative analysis.
27548134	2	12	theme	injections	308:317	arg1	one					284:286	one	284:286	one	284:286	This study aims to investigate the chemical composition of one of the best-selling injections, Shenqi Fuzheng (SQ) injection (SQI), via a full component quantitative analysis.
27548134	2	12	theme	injections	308:317	arg1	injection					340:348	Shenqi Fuzheng (SQ) injection	320:348	Shenqi Fuzheng (SQ) injection (SQI)	320:354	This study aims to investigate the chemical composition of one of the best-selling injections, Shenqi Fuzheng (SQ) injection (SQI), via a full component quantitative analysis.
27548134	2	12	theme	injections	308:317	arg1	injections					308:317	the best-selling injections	291:317	the best-selling injections	291:317	This study aims to investigate the chemical composition of one of the best-selling injections, Shenqi Fuzheng (SQ) injection (SQI), via a full component quantitative analysis.
27548134	3	13	theme	quadrupole	567:576	arg1	spectrometry					605:616	quadrupole tandem time-of-flight mass spectrometry	567:616	quadrupole tandem time-of-flight mass spectrometry (Q-TOF-MS)	567:627	A total of 15 representative small molecular components of SQI were simultaneously determined using ultra-high performance liquid chromatography (UHPLC) coupled with quadrupole tandem time-of-flight mass spectrometry (Q-TOF-MS); saccharide composition of SQI was also quantitatively determined by high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) on an amino column before and after acid hydrolysis.
27548134	3	13	theme	quadrupole	567:576	arg1	Q-TOF-MS					619:626	Q-TOF-MS	619:626	Q-TOF-MS	619:626	A total of 15 representative small molecular components of SQI were simultaneously determined using ultra-high performance liquid chromatography (UHPLC) coupled with quadrupole tandem time-of-flight mass spectrometry (Q-TOF-MS); saccharide composition of SQI was also quantitatively determined by high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) on an amino column before and after acid hydrolysis.
27548134	5	14	theme	SQI	1085:1087	arg1	samples					1089:1095	13 SQI samples	1082:1095	13 SQI samples	1082:1095	The method was well validated in terms of linearity, sensitivity, precision, accuracy and stability, and was successfully applied to analyze 13 SQI samples.
27548134	7	15	theme	therapy	1414:1420	arg1	efficacy					1422:1429	therapy efficacy	1414:1429	therapy efficacy	1414:1429	The quantitative information contributes to accumulating scientific evidence to better understand the therapy efficacy and safety of complex Chinese medicine injections.
27548134	5	16	theme	sensitivity	994:1004	arg1	terms					974:978	terms	974:978	terms of linearity, sensitivity, precision, accuracy and stability	974:1039	The method was well validated in terms of linearity, sensitivity, precision, accuracy and stability, and was successfully applied to analyze 13 SQI samples.
27548134	2	17	theme	component	368:376	arg1	analysis					391:398	a full component quantitative analysis	361:398	a full component quantitative analysis	361:398	This study aims to investigate the chemical composition of one of the best-selling injections, Shenqi Fuzheng (SQ) injection (SQI), via a full component quantitative analysis.
27548134	2	18	theme	one	284:286	arg1	composition					269:279	the chemical composition	256:279	the chemical composition of one of the best-selling injections, Shenqi Fuzheng (SQ) injection (SQI),	256:355	This study aims to investigate the chemical composition of one of the best-selling injections, Shenqi Fuzheng (SQ) injection (SQI), via a full component quantitative analysis.
27548134	2	19	theme	full	363:366	arg1	analysis					391:398	a full component quantitative analysis	361:398	a full component quantitative analysis	361:398	This study aims to investigate the chemical composition of one of the best-selling injections, Shenqi Fuzheng (SQ) injection (SQI), via a full component quantitative analysis.
27548134	0	20	theme	Quantitative	14:25	arg1	Analysis					27:34	Comprehensive Quantitative Analysis	0:34	Comprehensive Quantitative Analysis of SQ Injection	0:50	Comprehensive Quantitative Analysis of SQ Injection Using Multiple Chromatographic Technologies.
27548134	2	21	theme	Fuzheng	327:333	arg1	SQI					351:353	SQI	351:353	SQI	351:353	This study aims to investigate the chemical composition of one of the best-selling injections, Shenqi Fuzheng (SQ) injection (SQI), via a full component quantitative analysis.
27548134	2	21	theme	Fuzheng	327:333	arg1	one					284:286	one	284:286	one	284:286	This study aims to investigate the chemical composition of one of the best-selling injections, Shenqi Fuzheng (SQ) injection (SQI), via a full component quantitative analysis.
27548134	2	21	theme	Fuzheng	327:333	arg1	injection					340:348	Shenqi Fuzheng (SQ) injection	320:348	Shenqi Fuzheng (SQ) injection (SQI)	320:354	This study aims to investigate the chemical composition of one of the best-selling injections, Shenqi Fuzheng (SQ) injection (SQI), via a full component quantitative analysis.
27548134	2	21	theme	Fuzheng	327:333	arg1	injections					308:317	the best-selling injections	291:317	the best-selling injections	291:317	This study aims to investigate the chemical composition of one of the best-selling injections, Shenqi Fuzheng (SQ) injection (SQI), via a full component quantitative analysis.
27548134	0	22	theme	Comprehensive	0:12	arg1	Analysis					27:34	Comprehensive Quantitative Analysis	0:34	Comprehensive Quantitative Analysis of SQ Injection	0:50	Comprehensive Quantitative Analysis of SQ Injection Using Multiple Chromatographic Technologies.
27548134	3	23	theme	scattering	767:776	arg1	ELSD					788:791	ELSD	788:791	ELSD	788:791	A total of 15 representative small molecular components of SQI were simultaneously determined using ultra-high performance liquid chromatography (UHPLC) coupled with quadrupole tandem time-of-flight mass spectrometry (Q-TOF-MS); saccharide composition of SQI was also quantitatively determined by high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) on an amino column before and after acid hydrolysis.
27548134	3	23	theme	scattering	767:776	arg1	detector					778:785	evaporative light scattering detector	749:785	evaporative light scattering detector (ELSD)	749:792	A total of 15 representative small molecular components of SQI were simultaneously determined using ultra-high performance liquid chromatography (UHPLC) coupled with quadrupole tandem time-of-flight mass spectrometry (Q-TOF-MS); saccharide composition of SQI was also quantitatively determined by high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) on an amino column before and after acid hydrolysis.
27548134	6	24	theme	small	1212:1216	arg1	molecules					1218:1226	small molecules	1212:1226	small molecules	1212:1226	The results demonstrate that up to 94.69% (w/w) of this injection product are quantitatively determined, in which small molecules and monosaccharide/sucrose account for 0.18%-0.21%, and 53.49%-58.2%, respectively.
27548134	2	25	theme	Shenqi	320:325	arg1	SQI					351:353	SQI	351:353	SQI	351:353	This study aims to investigate the chemical composition of one of the best-selling injections, Shenqi Fuzheng (SQ) injection (SQI), via a full component quantitative analysis.
27548134	2	25	theme	Shenqi	320:325	arg1	one					284:286	one	284:286	one	284:286	This study aims to investigate the chemical composition of one of the best-selling injections, Shenqi Fuzheng (SQ) injection (SQI), via a full component quantitative analysis.
27548134	2	25	theme	Shenqi	320:325	arg1	injection					340:348	Shenqi Fuzheng (SQ) injection	320:348	Shenqi Fuzheng (SQ) injection (SQI)	320:354	This study aims to investigate the chemical composition of one of the best-selling injections, Shenqi Fuzheng (SQ) injection (SQI), via a full component quantitative analysis.
27548134	2	25	theme	Shenqi	320:325	arg1	injections					308:317	the best-selling injections	291:317	the best-selling injections	291:317	This study aims to investigate the chemical composition of one of the best-selling injections, Shenqi Fuzheng (SQ) injection (SQI), via a full component quantitative analysis.
27548134	1	26	theme	complex	199:205	arg1	profile					216:222	their complex chemical profile	193:222	their complex chemical profile	193:222	Quality control of Chinese medicine injections remains a challenge due to our poor knowledge of their complex chemical profile.
27548134	3	27	with	chromatography	722:735	arg1	ELSD					788:791	ELSD	788:791	ELSD	788:791	A total of 15 representative small molecular components of SQI were simultaneously determined using ultra-high performance liquid chromatography (UHPLC) coupled with quadrupole tandem time-of-flight mass spectrometry (Q-TOF-MS); saccharide composition of SQI was also quantitatively determined by high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) on an amino column before and after acid hydrolysis.
27548134	3	27	with	chromatography	722:735	arg1	detector					778:785	evaporative light scattering detector	749:785	evaporative light scattering detector (ELSD)	749:792	A total of 15 representative small molecular components of SQI were simultaneously determined using ultra-high performance liquid chromatography (UHPLC) coupled with quadrupole tandem time-of-flight mass spectrometry (Q-TOF-MS); saccharide composition of SQI was also quantitatively determined by high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) on an amino column before and after acid hydrolysis.
27548134	6	28	theme	product	1164:1170	arg1	%					1138:1138	up to 94.69%	1127:1138	up to 94.69% (w/w) of this injection product	1127:1170	The results demonstrate that up to 94.69% (w/w) of this injection product are quantitatively determined, in which small molecules and monosaccharide/sucrose account for 0.18%-0.21%, and 53.49%-58.2%, respectively.
27548134	6	28	theme	product	1164:1170	arg1	product					1164:1170	this injection product	1149:1170	this injection product	1149:1170	The results demonstrate that up to 94.69% (w/w) of this injection product are quantitatively determined, in which small molecules and monosaccharide/sucrose account for 0.18%-0.21%, and 53.49%-58.2%, respectively.
27548134	6	28	theme	product	1164:1170	arg1	w/w					1141:1143	w/w	1141:1143	w/w	1141:1143	The results demonstrate that up to 94.69% (w/w) of this injection product are quantitatively determined, in which small molecules and monosaccharide/sucrose account for 0.18%-0.21%, and 53.49%-58.2%, respectively.
27548134	5	29	theme	stability	1031:1039	arg1	terms					974:978	terms	974:978	terms of linearity, sensitivity, precision, accuracy and stability	974:1039	The method was well validated in terms of linearity, sensitivity, precision, accuracy and stability, and was successfully applied to analyze 13 SQI samples.
27548134	1	30	theme	Chinese	116:122	arg1	injections					133:142	Chinese medicine injections	116:142	Chinese medicine injections	116:142	Quality control of Chinese medicine injections remains a challenge due to our poor knowledge of their complex chemical profile.
27548134	1	31	theme	chemical	207:214	arg1	profile					216:222	their complex chemical profile	193:222	their complex chemical profile	193:222	Quality control of Chinese medicine injections remains a challenge due to our poor knowledge of their complex chemical profile.
27548134	3	32	theme	tandem	578:583	arg1	spectrometry					605:616	quadrupole tandem time-of-flight mass spectrometry	567:616	quadrupole tandem time-of-flight mass spectrometry (Q-TOF-MS)	567:627	A total of 15 representative small molecular components of SQI were simultaneously determined using ultra-high performance liquid chromatography (UHPLC) coupled with quadrupole tandem time-of-flight mass spectrometry (Q-TOF-MS); saccharide composition of SQI was also quantitatively determined by high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) on an amino column before and after acid hydrolysis.
27548134	3	32	theme	tandem	578:583	arg1	Q-TOF-MS					619:626	Q-TOF-MS	619:626	Q-TOF-MS	619:626	A total of 15 representative small molecular components of SQI were simultaneously determined using ultra-high performance liquid chromatography (UHPLC) coupled with quadrupole tandem time-of-flight mass spectrometry (Q-TOF-MS); saccharide composition of SQI was also quantitatively determined by high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) on an amino column before and after acid hydrolysis.
27548134	3	33	theme	evaporative	749:759	arg1	ELSD					788:791	ELSD	788:791	ELSD	788:791	A total of 15 representative small molecular components of SQI were simultaneously determined using ultra-high performance liquid chromatography (UHPLC) coupled with quadrupole tandem time-of-flight mass spectrometry (Q-TOF-MS); saccharide composition of SQI was also quantitatively determined by high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) on an amino column before and after acid hydrolysis.
27548134	3	33	theme	evaporative	749:759	arg1	detector					778:785	evaporative light scattering detector	749:785	evaporative light scattering detector (ELSD)	749:792	A total of 15 representative small molecular components of SQI were simultaneously determined using ultra-high performance liquid chromatography (UHPLC) coupled with quadrupole tandem time-of-flight mass spectrometry (Q-TOF-MS); saccharide composition of SQI was also quantitatively determined by high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) on an amino column before and after acid hydrolysis.
27548134	4	34	theme	chromatography	918:931	arg1	column					933:938	a gel permeation chromatography column	901:938	a gel permeation chromatography column	901:938	The existence of polysaccharides was also examined on a gel permeation chromatography column.
27548134	3	35	theme	SQI	656:658	arg1	composition					641:651	saccharide composition	630:651	saccharide composition of SQI	630:658	A total of 15 representative small molecular components of SQI were simultaneously determined using ultra-high performance liquid chromatography (UHPLC) coupled with quadrupole tandem time-of-flight mass spectrometry (Q-TOF-MS); saccharide composition of SQI was also quantitatively determined by high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) on an amino column before and after acid hydrolysis.
27548134	1	36	theme	medicine	124:131	arg1	injections					133:142	Chinese medicine injections	116:142	Chinese medicine injections	116:142	Quality control of Chinese medicine injections remains a challenge due to our poor knowledge of their complex chemical profile.
27548134	1	37	theme	profile	216:222	arg1	knowledge					180:188	our poor knowledge	171:188	our poor knowledge of their complex chemical profile	171:222	Quality control of Chinese medicine injections remains a challenge due to our poor knowledge of their complex chemical profile.
27548134	0	38	theme	Injection	42:50	arg1	Analysis					27:34	Comprehensive Quantitative Analysis	0:34	Comprehensive Quantitative Analysis of SQ Injection	0:50	Comprehensive Quantitative Analysis of SQ Injection Using Multiple Chromatographic Technologies.
27548134	3	39	theme	light	761:765	arg1	ELSD					788:791	ELSD	788:791	ELSD	788:791	A total of 15 representative small molecular components of SQI were simultaneously determined using ultra-high performance liquid chromatography (UHPLC) coupled with quadrupole tandem time-of-flight mass spectrometry (Q-TOF-MS); saccharide composition of SQI was also quantitatively determined by high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) on an amino column before and after acid hydrolysis.
27548134	3	39	theme	light	761:765	arg1	detector					778:785	evaporative light scattering detector	749:785	evaporative light scattering detector (ELSD)	749:792	A total of 15 representative small molecular components of SQI were simultaneously determined using ultra-high performance liquid chromatography (UHPLC) coupled with quadrupole tandem time-of-flight mass spectrometry (Q-TOF-MS); saccharide composition of SQI was also quantitatively determined by high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) on an amino column before and after acid hydrolysis.
27548134	6	40	theme	%	1289:1289	arg1	%					1295:1295	53.49%-58.2%	1284:1295	53.49%-58.2%	1284:1295	The results demonstrate that up to 94.69% (w/w) of this injection product are quantitatively determined, in which small molecules and monosaccharide/sucrose account for 0.18%-0.21%, and 53.49%-58.2%, respectively.
27548134	3	41	theme	small	430:434	arg1	components					446:455	15 representative small molecular components	412:455	15 representative small molecular components of SQI	412:462	A total of 15 representative small molecular components of SQI were simultaneously determined using ultra-high performance liquid chromatography (UHPLC) coupled with quadrupole tandem time-of-flight mass spectrometry (Q-TOF-MS); saccharide composition of SQI was also quantitatively determined by high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) on an amino column before and after acid hydrolysis.
27548134	0	42	theme	SQ	39:40	arg1	Injection					42:50	SQ Injection	39:50	SQ Injection	39:50	Comprehensive Quantitative Analysis of SQ Injection Using Multiple Chromatographic Technologies.
27548134	6	43	theme	0.18	1267:1270	arg1	%					1277:1277	0.18%-0.21%	1267:1277	0.18%-0.21%	1267:1277	The results demonstrate that up to 94.69% (w/w) of this injection product are quantitatively determined, in which small molecules and monosaccharide/sucrose account for 0.18%-0.21%, and 53.49%-58.2%, respectively.
27548134	6	44	theme	53.49	1284:1288	arg1	%					1295:1295	53.49%-58.2%	1284:1295	53.49%-58.2%	1284:1295	The results demonstrate that up to 94.69% (w/w) of this injection product are quantitatively determined, in which small molecules and monosaccharide/sucrose account for 0.18%-0.21%, and 53.49%-58.2%, respectively.
27548134	5	45	theme	accuracy	1018:1025	arg1	terms					974:978	terms	974:978	terms of linearity, sensitivity, precision, accuracy and stability	974:1039	The method was well validated in terms of linearity, sensitivity, precision, accuracy and stability, and was successfully applied to analyze 13 SQI samples.
27548134	2	46	theme	quantitative	378:389	arg1	analysis					391:398	a full component quantitative analysis	361:398	a full component quantitative analysis	361:398	This study aims to investigate the chemical composition of one of the best-selling injections, Shenqi Fuzheng (SQ) injection (SQI), via a full component quantitative analysis.
27548134	0	47	theme	Multiple	58:65	arg1	Technologies					83:94	Multiple Chromatographic Technologies	58:94	Multiple Chromatographic Technologies	58:94	Comprehensive Quantitative Analysis of SQ Injection Using Multiple Chromatographic Technologies.
27548134	7	48	dep	efficacy	1422:1429	arg1	the					1410:1412	the	1410:1412	the	1410:1412	The quantitative information contributes to accumulating scientific evidence to better understand the therapy efficacy and safety of complex Chinese medicine injections.
27548134	3	49	theme	time-of-flight	585:598	arg1	spectrometry					605:616	quadrupole tandem time-of-flight mass spectrometry	567:616	quadrupole tandem time-of-flight mass spectrometry (Q-TOF-MS)	567:627	A total of 15 representative small molecular components of SQI were simultaneously determined using ultra-high performance liquid chromatography (UHPLC) coupled with quadrupole tandem time-of-flight mass spectrometry (Q-TOF-MS); saccharide composition of SQI was also quantitatively determined by high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) on an amino column before and after acid hydrolysis.
27548134	3	49	theme	time-of-flight	585:598	arg1	Q-TOF-MS					619:626	Q-TOF-MS	619:626	Q-TOF-MS	619:626	A total of 15 representative small molecular components of SQI were simultaneously determined using ultra-high performance liquid chromatography (UHPLC) coupled with quadrupole tandem time-of-flight mass spectrometry (Q-TOF-MS); saccharide composition of SQI was also quantitatively determined by high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) on an amino column before and after acid hydrolysis.
27548134	1	50	theme	injections	133:142	arg1	control					105:111	Quality control	97:111	Quality control of Chinese medicine injections	97:142	Quality control of Chinese medicine injections remains a challenge due to our poor knowledge of their complex chemical profile.
27548134	3	51	from	chromatography	722:735	arg1	column					806:811	an amino column	797:811	an amino column before and after acid hydrolysis	797:844	A total of 15 representative small molecular components of SQI were simultaneously determined using ultra-high performance liquid chromatography (UHPLC) coupled with quadrupole tandem time-of-flight mass spectrometry (Q-TOF-MS); saccharide composition of SQI was also quantitatively determined by high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) on an amino column before and after acid hydrolysis.
27548134	6	52	dep	94.69	1133:1137	arg1	to					1130:1131	to	1130:1131	to	1130:1131	The results demonstrate that up to 94.69% (w/w) of this injection product are quantitatively determined, in which small molecules and monosaccharide/sucrose account for 0.18%-0.21%, and 53.49%-58.2%, respectively.
27548134	7	53	theme	medicine	1461:1468	arg1	injections					1470:1479	complex Chinese medicine injections	1445:1479	complex Chinese medicine injections	1445:1479	The quantitative information contributes to accumulating scientific evidence to better understand the therapy efficacy and safety of complex Chinese medicine injections.
27548134	3	54	theme	mass	600:603	arg1	spectrometry					605:616	quadrupole tandem time-of-flight mass spectrometry	567:616	quadrupole tandem time-of-flight mass spectrometry (Q-TOF-MS)	567:627	A total of 15 representative small molecular components of SQI were simultaneously determined using ultra-high performance liquid chromatography (UHPLC) coupled with quadrupole tandem time-of-flight mass spectrometry (Q-TOF-MS); saccharide composition of SQI was also quantitatively determined by high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) on an amino column before and after acid hydrolysis.
27548134	3	54	theme	mass	600:603	arg1	Q-TOF-MS					619:626	Q-TOF-MS	619:626	Q-TOF-MS	619:626	A total of 15 representative small molecular components of SQI were simultaneously determined using ultra-high performance liquid chromatography (UHPLC) coupled with quadrupole tandem time-of-flight mass spectrometry (Q-TOF-MS); saccharide composition of SQI was also quantitatively determined by high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) on an amino column before and after acid hydrolysis.
27548134	3	55	theme	ultra-high	501:510	arg1	UHPLC					547:551	UHPLC	547:551	UHPLC	547:551	A total of 15 representative small molecular components of SQI were simultaneously determined using ultra-high performance liquid chromatography (UHPLC) coupled with quadrupole tandem time-of-flight mass spectrometry (Q-TOF-MS); saccharide composition of SQI was also quantitatively determined by high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) on an amino column before and after acid hydrolysis.
27548134	3	55	theme	ultra-high	501:510	arg1	chromatography					531:544	ultra-high performance liquid chromatography	501:544	ultra-high performance liquid chromatography (UHPLC) coupled with quadrupole tandem time-of-flight mass spectrometry (Q-TOF-MS)	501:627	A total of 15 representative small molecular components of SQI were simultaneously determined using ultra-high performance liquid chromatography (UHPLC) coupled with quadrupole tandem time-of-flight mass spectrometry (Q-TOF-MS); saccharide composition of SQI was also quantitatively determined by high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) on an amino column before and after acid hydrolysis.
27548134	7	56	theme	Chinese	1453:1459	arg1	injections					1470:1479	complex Chinese medicine injections	1445:1479	complex Chinese medicine injections	1445:1479	The quantitative information contributes to accumulating scientific evidence to better understand the therapy efficacy and safety of complex Chinese medicine injections.
27548134	6	57	theme	injection	1154:1162	arg1	product					1164:1170	this injection product	1149:1170	this injection product	1149:1170	The results demonstrate that up to 94.69% (w/w) of this injection product are quantitatively determined, in which small molecules and monosaccharide/sucrose account for 0.18%-0.21%, and 53.49%-58.2%, respectively.
27548134	4	58	theme	permeation	907:916	arg1	column					933:938	a gel permeation chromatography column	901:938	a gel permeation chromatography column	901:938	The existence of polysaccharides was also examined on a gel permeation chromatography column.
27548134	2	59	theme	SQ	336:337	arg1	SQI					351:353	SQI	351:353	SQI	351:353	This study aims to investigate the chemical composition of one of the best-selling injections, Shenqi Fuzheng (SQ) injection (SQI), via a full component quantitative analysis.
27548134	2	59	theme	SQ	336:337	arg1	one					284:286	one	284:286	one	284:286	This study aims to investigate the chemical composition of one of the best-selling injections, Shenqi Fuzheng (SQ) injection (SQI), via a full component quantitative analysis.
27548134	2	59	theme	SQ	336:337	arg1	injection					340:348	Shenqi Fuzheng (SQ) injection	320:348	Shenqi Fuzheng (SQ) injection (SQI)	320:354	This study aims to investigate the chemical composition of one of the best-selling injections, Shenqi Fuzheng (SQ) injection (SQI), via a full component quantitative analysis.
27548134	2	59	theme	SQ	336:337	arg1	injections					308:317	the best-selling injections	291:317	the best-selling injections	291:317	This study aims to investigate the chemical composition of one of the best-selling injections, Shenqi Fuzheng (SQ) injection (SQI), via a full component quantitative analysis.
27548134	7	60	theme	scientific	1369:1378	arg1	evidence					1380:1387	scientific evidence to better understand the therapy efficacy and safety of complex Chinese medicine injections	1369:1479	scientific evidence to better understand the therapy efficacy and safety of complex Chinese medicine injections	1369:1479	The quantitative information contributes to accumulating scientific evidence to better understand the therapy efficacy and safety of complex Chinese medicine injections.
27548134	1	61	theme	Quality	97:103	arg1	control					105:111	Quality control	97:111	Quality control of Chinese medicine injections	97:142	Quality control of Chinese medicine injections remains a challenge due to our poor knowledge of their complex chemical profile.
27548134	3	62	theme	saccharide	630:639	arg1	composition					641:651	saccharide composition	630:651	saccharide composition of SQI	630:658	A total of 15 representative small molecular components of SQI were simultaneously determined using ultra-high performance liquid chromatography (UHPLC) coupled with quadrupole tandem time-of-flight mass spectrometry (Q-TOF-MS); saccharide composition of SQI was also quantitatively determined by high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) on an amino column before and after acid hydrolysis.
27548134	3	63	theme	SQI	460:462	arg1	components					446:455	15 representative small molecular components	412:455	15 representative small molecular components of SQI	412:462	A total of 15 representative small molecular components of SQI were simultaneously determined using ultra-high performance liquid chromatography (UHPLC) coupled with quadrupole tandem time-of-flight mass spectrometry (Q-TOF-MS); saccharide composition of SQI was also quantitatively determined by high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) on an amino column before and after acid hydrolysis.
27548134	3	64	theme	molecular	436:444	arg1	components					446:455	15 representative small molecular components	412:455	15 representative small molecular components of SQI	412:462	A total of 15 representative small molecular components of SQI were simultaneously determined using ultra-high performance liquid chromatography (UHPLC) coupled with quadrupole tandem time-of-flight mass spectrometry (Q-TOF-MS); saccharide composition of SQI was also quantitatively determined by high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) on an amino column before and after acid hydrolysis.
27548134	2	65	theme	chemical	260:267	arg1	composition					269:279	the chemical composition	256:279	the chemical composition of one of the best-selling injections, Shenqi Fuzheng (SQ) injection (SQI),	256:355	This study aims to investigate the chemical composition of one of the best-selling injections, Shenqi Fuzheng (SQ) injection (SQI), via a full component quantitative analysis.
27548134	3	66	theme	components	446:455	arg1	total					403:407	A total	401:407	A total of 15 representative small molecular components of SQI	401:462	A total of 15 representative small molecular components of SQI were simultaneously determined using ultra-high performance liquid chromatography (UHPLC) coupled with quadrupole tandem time-of-flight mass spectrometry (Q-TOF-MS); saccharide composition of SQI was also quantitatively determined by high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) on an amino column before and after acid hydrolysis.
27548134	1	67	theme	due	164:166	arg1	challenge					154:162	a challenge	152:162	a challenge due to our poor knowledge of their complex chemical profile	152:222	Quality control of Chinese medicine injections remains a challenge due to our poor knowledge of their complex chemical profile.
27548134	3	68	theme	representative	415:428	arg1	components					446:455	15 representative small molecular components	412:455	15 representative small molecular components of SQI	412:462	A total of 15 representative small molecular components of SQI were simultaneously determined using ultra-high performance liquid chromatography (UHPLC) coupled with quadrupole tandem time-of-flight mass spectrometry (Q-TOF-MS); saccharide composition of SQI was also quantitatively determined by high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) on an amino column before and after acid hydrolysis.
27548134	7	69	theme	complex	1445:1451	arg1	injections					1470:1479	complex Chinese medicine injections	1445:1479	complex Chinese medicine injections	1445:1479	The quantitative information contributes to accumulating scientific evidence to better understand the therapy efficacy and safety of complex Chinese medicine injections.
27548134	0	70	theme	Chromatographic	67:81	arg1	Technologies					83:94	Multiple Chromatographic Technologies	58:94	Multiple Chromatographic Technologies	58:94	Comprehensive Quantitative Analysis of SQ Injection Using Multiple Chromatographic Technologies.
27548134	3	71	theme	liquid	715:720	arg1	HPLC					738:741	HPLC	738:741	HPLC	738:741	A total of 15 representative small molecular components of SQI were simultaneously determined using ultra-high performance liquid chromatography (UHPLC) coupled with quadrupole tandem time-of-flight mass spectrometry (Q-TOF-MS); saccharide composition of SQI was also quantitatively determined by high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) on an amino column before and after acid hydrolysis.
27548134	3	71	theme	liquid	715:720	arg1	chromatography					722:735	high performance liquid chromatography	698:735	high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) on an amino column before and after acid hydrolysis	698:844	A total of 15 representative small molecular components of SQI were simultaneously determined using ultra-high performance liquid chromatography (UHPLC) coupled with quadrupole tandem time-of-flight mass spectrometry (Q-TOF-MS); saccharide composition of SQI was also quantitatively determined by high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) on an amino column before and after acid hydrolysis.
27548134	5	72	theme	precision	1007:1015	arg1	terms					974:978	terms	974:978	terms of linearity, sensitivity, precision, accuracy and stability	974:1039	The method was well validated in terms of linearity, sensitivity, precision, accuracy and stability, and was successfully applied to analyze 13 SQI samples.
27548134	3	73	theme	liquid	524:529	arg1	UHPLC					547:551	UHPLC	547:551	UHPLC	547:551	A total of 15 representative small molecular components of SQI were simultaneously determined using ultra-high performance liquid chromatography (UHPLC) coupled with quadrupole tandem time-of-flight mass spectrometry (Q-TOF-MS); saccharide composition of SQI was also quantitatively determined by high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) on an amino column before and after acid hydrolysis.
27548134	3	73	theme	liquid	524:529	arg1	chromatography					531:544	ultra-high performance liquid chromatography	501:544	ultra-high performance liquid chromatography (UHPLC) coupled with quadrupole tandem time-of-flight mass spectrometry (Q-TOF-MS)	501:627	A total of 15 representative small molecular components of SQI were simultaneously determined using ultra-high performance liquid chromatography (UHPLC) coupled with quadrupole tandem time-of-flight mass spectrometry (Q-TOF-MS); saccharide composition of SQI was also quantitatively determined by high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) on an amino column before and after acid hydrolysis.
27548134	3	74	theme	amino	800:804	arg1	column					806:811	an amino column	797:811	an amino column before and after acid hydrolysis	797:844	A total of 15 representative small molecular components of SQI were simultaneously determined using ultra-high performance liquid chromatography (UHPLC) coupled with quadrupole tandem time-of-flight mass spectrometry (Q-TOF-MS); saccharide composition of SQI was also quantitatively determined by high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) on an amino column before and after acid hydrolysis.
29168717	3	0	theme	mL	644:645	arg1	solution					695:702	50 mL Diamozol Blue BRF %150 (C.I. Reactive Blue 221) solution	641:702	50 mL Diamozol Blue BRF %150 (C.I. Reactive Blue 221) solution with fixed concentrations (60 mg/L)	641:738	To evaluate the adsorption behaviour of the chitosan/marble powder composites, 0.1 g adsorbent was added into 50 mL Diamozol Blue BRF %150 (C.I. Reactive Blue 221) solution with fixed concentrations (60 mg/L).
29168717	7	1	theme	isotherm	1352:1359	arg1	models					1361:1366	the pseudo-second-order and Freundlich isotherm models	1313:1366	the pseudo-second-order and Freundlich isotherm models	1313:1366	The studies revealed that Dimozol Blue dye adsorption was described well by the pseudo-second-order and Freundlich isotherm models.
29168717	2	2	theme	surface	321:327	arg1	area					329:332	the surface area	317:332	the surface area of the chitosan/marble powder composites	317:373	The morphology and the surface area of the chitosan/marble powder composites were also characterized with a scanning electron microscope (SEM) and Micromeritics (ASAP 2020) BET (Brunauer, Emmett and Teller) instrument, respectively.
29168717	5	3	theme	marble	970:975	arg1	content					984:990	the higher marble powder content	959:990	the higher marble powder content	959:990	C50M50 composite was more economical than C100M0 and C70M30 due to the higher marble powder content, and hence was selected as an adsorbent for the removal of Dimozol Blue from aqueous solution.
29168717	5	4	theme	Blue	1059:1062	arg1	Dimozol					1051:1057	Dimozol Blue	1051:1062	Dimozol Blue	1051:1062	C50M50 composite was more economical than C100M0 and C70M30 due to the higher marble powder content, and hence was selected as an adsorbent for the removal of Dimozol Blue from aqueous solution.
29168717	8	5	theme	study	1389:1393	arg1	results					1373:1379	The results	1369:1379	The results of this study	1369:1393	The results of this study indicated the applicability of the chitosan/marble powder composites for removing industrial dyes from aqueous solution.
29168717	4	6	theme	adsorption	761:770	arg1	mg/g					840:843	about 27 mg/g	831:843	about 27 mg/g	831:843	At equilibrium, the adsorption capacity of C100M0, C70M30 and C50M50 for Dimozol Blue was about 27 mg/g and significantly greater than that of C30M70.
29168717	4	6	theme	adsorption	761:770	arg1	capacity					772:779	the adsorption capacity	757:779	the adsorption capacity of C100M0, C70M30 and C50M50 for Dimozol Blue	757:825	At equilibrium, the adsorption capacity of C100M0, C70M30 and C50M50 for Dimozol Blue was about 27 mg/g and significantly greater than that of C30M70.
29168717	1	7	theme	different	142:150	arg1	percentage					165:174	different weight ratio percentage	142:174	different weight ratio percentage (C100M0, C70M30, C50M50, and C30M70)	142:211	Cross-linked chitosan(C)/marble powder (M) composites with different weight ratio percentage (C100M0, C70M30, C50M50, and C30M70) were prepared from marble powder and chitosan and cross-linked using glutaraldehyde.
29168717	4	8	theme	C100M0	784:789	arg1	mg/g					840:843	about 27 mg/g	831:843	about 27 mg/g	831:843	At equilibrium, the adsorption capacity of C100M0, C70M30 and C50M50 for Dimozol Blue was about 27 mg/g and significantly greater than that of C30M70.
29168717	4	8	theme	C100M0	784:789	arg1	capacity					772:779	the adsorption capacity	757:779	the adsorption capacity of C100M0, C70M30 and C50M50 for Dimozol Blue	757:825	At equilibrium, the adsorption capacity of C100M0, C70M30 and C50M50 for Dimozol Blue was about 27 mg/g and significantly greater than that of C30M70.
29168717	7	9	theme	pseudo-second-order	1317:1335	arg1	models					1361:1366	the pseudo-second-order and Freundlich isotherm models	1313:1366	the pseudo-second-order and Freundlich isotherm models	1313:1366	The studies revealed that Dimozol Blue dye adsorption was described well by the pseudo-second-order and Freundlich isotherm models.
29168717	5	10	theme	higher	963:968	arg1	content					984:990	the higher marble powder content	959:990	the higher marble powder content	959:990	C50M50 composite was more economical than C100M0 and C70M30 due to the higher marble powder content, and hence was selected as an adsorbent for the removal of Dimozol Blue from aqueous solution.
29168717	5	11	theme	aqueous	1069:1075	arg1	solution					1077:1084	aqueous solution	1069:1084	aqueous solution	1069:1084	C50M50 composite was more economical than C100M0 and C70M30 due to the higher marble powder content, and hence was selected as an adsorbent for the removal of Dimozol Blue from aqueous solution.
29168717	1	12	link	Cross-linked	83:94	arg1	M					123:123	M	123:123	M	123:123	Cross-linked chitosan(C)/marble powder (M) composites with different weight ratio percentage (C100M0, C70M30, C50M50, and C30M70) were prepared from marble powder and chitosan and cross-linked using glutaraldehyde.
29168717	1	12	link	Cross-linked	83:94	arg1	powder					115:120	Cross-linked chitosan(C)/marble powder	83:120	Cross-linked chitosan(C)/marble powder (M) composites with different weight ratio percentage (C100M0, C70M30, C50M50, and C30M70)	83:211	Cross-linked chitosan(C)/marble powder (M) composites with different weight ratio percentage (C100M0, C70M30, C50M50, and C30M70) were prepared from marble powder and chitosan and cross-linked using glutaraldehyde.
29168717	3	13	theme	BRF	661:663	arg1	221					690:692	C.I. Reactive Blue 221	671:692	C.I. Reactive Blue 221	671:692	To evaluate the adsorption behaviour of the chitosan/marble powder composites, 0.1 g adsorbent was added into 50 mL Diamozol Blue BRF %150 (C.I. Reactive Blue 221) solution with fixed concentrations (60 mg/L).
29168717	3	13	theme	BRF	661:663	arg1	%					665:665	Diamozol Blue BRF %150	647:668	50 mL Diamozol Blue BRF %150 (C.I. Reactive Blue 221) solution with fixed concentrations (60 mg/L)	641:738	To evaluate the adsorption behaviour of the chitosan/marble powder composites, 0.1 g adsorbent was added into 50 mL Diamozol Blue BRF %150 (C.I. Reactive Blue 221) solution with fixed concentrations (60 mg/L).
29168717	1	14	with	composites	126:135	arg1	percentage					165:174	different weight ratio percentage	142:174	different weight ratio percentage (C100M0, C70M30, C50M50, and C30M70)	142:211	Cross-linked chitosan(C)/marble powder (M) composites with different weight ratio percentage (C100M0, C70M30, C50M50, and C30M70) were prepared from marble powder and chitosan and cross-linked using glutaraldehyde.
29168717	6	15	theme	Dimozol	1140:1146	arg1	isotherms					1127:1135	equilibrium isotherms	1115:1135	equilibrium isotherms	1115:1135	The adsorption kinetics and equilibrium isotherms of Dimozol Blue onto the chitosan/marble powder composites from aqueous solution were investigated.
29168717	6	15	theme	Dimozol	1140:1146	arg1	kinetics					1102:1109	adsorption kinetics	1091:1109	adsorption kinetics	1091:1109	The adsorption kinetics and equilibrium isotherms of Dimozol Blue onto the chitosan/marble powder composites from aqueous solution were investigated.
29168717	6	16	dep	kinetics	1102:1109	arg1	The					1087:1089	The	1087:1089	The	1087:1089	The adsorption kinetics and equilibrium isotherms of Dimozol Blue onto the chitosan/marble powder composites from aqueous solution were investigated.
29168717	3	17	theme	%	665:665	arg1	solution					695:702	50 mL Diamozol Blue BRF %150 (C.I. Reactive Blue 221) solution	641:702	50 mL Diamozol Blue BRF %150 (C.I. Reactive Blue 221) solution with fixed concentrations (60 mg/L)	641:738	To evaluate the adsorption behaviour of the chitosan/marble powder composites, 0.1 g adsorbent was added into 50 mL Diamozol Blue BRF %150 (C.I. Reactive Blue 221) solution with fixed concentrations (60 mg/L).
29168717	7	18	theme	dye	1276:1278	arg1	adsorption					1280:1289	Dimozol Blue dye adsorption	1263:1289	Dimozol Blue dye adsorption	1263:1289	The studies revealed that Dimozol Blue dye adsorption was described well by the pseudo-second-order and Freundlich isotherm models.
29168717	3	19	with	solution	695:702	arg1	concentrations					715:728	fixed concentrations	709:728	fixed concentrations (60 mg/L)	709:738	To evaluate the adsorption behaviour of the chitosan/marble powder composites, 0.1 g adsorbent was added into 50 mL Diamozol Blue BRF %150 (C.I. Reactive Blue 221) solution with fixed concentrations (60 mg/L).
29168717	3	19	with	solution	695:702	arg1	mg/L					734:737	60 mg/L	731:737	60 mg/L	731:737	To evaluate the adsorption behaviour of the chitosan/marble powder composites, 0.1 g adsorbent was added into 50 mL Diamozol Blue BRF %150 (C.I. Reactive Blue 221) solution with fixed concentrations (60 mg/L).
29168717	1	20	theme	weight	152:157	arg1	percentage					165:174	different weight ratio percentage	142:174	different weight ratio percentage (C100M0, C70M30, C50M50, and C30M70)	142:211	Cross-linked chitosan(C)/marble powder (M) composites with different weight ratio percentage (C100M0, C70M30, C50M50, and C30M70) were prepared from marble powder and chitosan and cross-linked using glutaraldehyde.
29168717	3	21	theme	Diamozol	647:654	arg1	221					690:692	C.I. Reactive Blue 221	671:692	C.I. Reactive Blue 221	671:692	To evaluate the adsorption behaviour of the chitosan/marble powder composites, 0.1 g adsorbent was added into 50 mL Diamozol Blue BRF %150 (C.I. Reactive Blue 221) solution with fixed concentrations (60 mg/L).
29168717	3	21	theme	Diamozol	647:654	arg1	%					665:665	Diamozol Blue BRF %150	647:668	50 mL Diamozol Blue BRF %150 (C.I. Reactive Blue 221) solution with fixed concentrations (60 mg/L)	641:738	To evaluate the adsorption behaviour of the chitosan/marble powder composites, 0.1 g adsorbent was added into 50 mL Diamozol Blue BRF %150 (C.I. Reactive Blue 221) solution with fixed concentrations (60 mg/L).
29168717	1	22	theme	Cross-linked	83:94	arg1	M					123:123	M	123:123	M	123:123	Cross-linked chitosan(C)/marble powder (M) composites with different weight ratio percentage (C100M0, C70M30, C50M50, and C30M70) were prepared from marble powder and chitosan and cross-linked using glutaraldehyde.
29168717	1	22	theme	Cross-linked	83:94	arg1	powder					115:120	Cross-linked chitosan(C)/marble powder	83:120	Cross-linked chitosan(C)/marble powder (M) composites with different weight ratio percentage (C100M0, C70M30, C50M50, and C30M70)	83:211	Cross-linked chitosan(C)/marble powder (M) composites with different weight ratio percentage (C100M0, C70M30, C50M50, and C30M70) were prepared from marble powder and chitosan and cross-linked using glutaraldehyde.
29168717	1	23	theme	ratio	159:163	arg1	percentage					165:174	different weight ratio percentage	142:174	different weight ratio percentage (C100M0, C70M30, C50M50, and C30M70)	142:211	Cross-linked chitosan(C)/marble powder (M) composites with different weight ratio percentage (C100M0, C70M30, C50M50, and C30M70) were prepared from marble powder and chitosan and cross-linked using glutaraldehyde.
29168717	0	24	theme	chitosan/marble	13:27	arg1	composites					36:45	chitosan/marble powder composites	13:45	chitosan/marble powder composites	13:45	Cross-linked chitosan/marble powder composites for the adsorption of Dimozol Blue.
29168717	3	25	theme	Blue	656:659	arg1	221					690:692	C.I. Reactive Blue 221	671:692	C.I. Reactive Blue 221	671:692	To evaluate the adsorption behaviour of the chitosan/marble powder composites, 0.1 g adsorbent was added into 50 mL Diamozol Blue BRF %150 (C.I. Reactive Blue 221) solution with fixed concentrations (60 mg/L).
29168717	3	25	theme	Blue	656:659	arg1	%					665:665	Diamozol Blue BRF %150	647:668	50 mL Diamozol Blue BRF %150 (C.I. Reactive Blue 221) solution with fixed concentrations (60 mg/L)	641:738	To evaluate the adsorption behaviour of the chitosan/marble powder composites, 0.1 g adsorbent was added into 50 mL Diamozol Blue BRF %150 (C.I. Reactive Blue 221) solution with fixed concentrations (60 mg/L).
29168717	2	26	dep	microscope	424:433	arg1	BET					471:473	BET	471:473	BET	471:473	The morphology and the surface area of the chitosan/marble powder composites were also characterized with a scanning electron microscope (SEM) and Micromeritics (ASAP 2020) BET (Brunauer, Emmett and Teller) instrument, respectively.
29168717	3	27	theme	C.I.	671:674	arg1	221					690:692	C.I. Reactive Blue 221	671:692	C.I. Reactive Blue 221	671:692	To evaluate the adsorption behaviour of the chitosan/marble powder composites, 0.1 g adsorbent was added into 50 mL Diamozol Blue BRF %150 (C.I. Reactive Blue 221) solution with fixed concentrations (60 mg/L).
29168717	3	27	theme	C.I.	671:674	arg1	%					665:665	Diamozol Blue BRF %150	647:668	50 mL Diamozol Blue BRF %150 (C.I. Reactive Blue 221) solution with fixed concentrations (60 mg/L)	641:738	To evaluate the adsorption behaviour of the chitosan/marble powder composites, 0.1 g adsorbent was added into 50 mL Diamozol Blue BRF %150 (C.I. Reactive Blue 221) solution with fixed concentrations (60 mg/L).
29168717	1	28	theme	chitosan	96:103	arg1	M					123:123	M	123:123	M	123:123	Cross-linked chitosan(C)/marble powder (M) composites with different weight ratio percentage (C100M0, C70M30, C50M50, and C30M70) were prepared from marble powder and chitosan and cross-linked using glutaraldehyde.
29168717	1	28	theme	chitosan	96:103	arg1	powder					115:120	Cross-linked chitosan(C)/marble powder	83:120	Cross-linked chitosan(C)/marble powder (M) composites with different weight ratio percentage (C100M0, C70M30, C50M50, and C30M70)	83:211	Cross-linked chitosan(C)/marble powder (M) composites with different weight ratio percentage (C100M0, C70M30, C50M50, and C30M70) were prepared from marble powder and chitosan and cross-linked using glutaraldehyde.
29168717	5	29	theme	powder	977:982	arg1	content					984:990	the higher marble powder content	959:990	the higher marble powder content	959:990	C50M50 composite was more economical than C100M0 and C70M30 due to the higher marble powder content, and hence was selected as an adsorbent for the removal of Dimozol Blue from aqueous solution.
29168717	2	30	theme	chitosan/marble	341:355	arg1	composites					364:373	the chitosan/marble powder composites	337:373	the chitosan/marble powder composites	337:373	The morphology and the surface area of the chitosan/marble powder composites were also characterized with a scanning electron microscope (SEM) and Micromeritics (ASAP 2020) BET (Brunauer, Emmett and Teller) instrument, respectively.
29168717	2	31	theme	electron	415:422	arg1	SEM					436:438	SEM	436:438	SEM	436:438	The morphology and the surface area of the chitosan/marble powder composites were also characterized with a scanning electron microscope (SEM) and Micromeritics (ASAP 2020) BET (Brunauer, Emmett and Teller) instrument, respectively.
29168717	2	31	theme	electron	415:422	arg1	microscope					424:433	a scanning electron microscope	404:433	a scanning electron microscope (SEM)	404:439	The morphology and the surface area of the chitosan/marble powder composites were also characterized with a scanning electron microscope (SEM) and Micromeritics (ASAP 2020) BET (Brunauer, Emmett and Teller) instrument, respectively.
29168717	1	32	theme	marble	232:237	arg1	powder					239:244	marble powder	232:244	marble powder	232:244	Cross-linked chitosan(C)/marble powder (M) composites with different weight ratio percentage (C100M0, C70M30, C50M50, and C30M70) were prepared from marble powder and chitosan and cross-linked using glutaraldehyde.
29168717	3	33	theme	adsorbent	616:624	arg1	g					614:614	0.1 g adsorbent	610:624	0.1 g adsorbent	610:624	To evaluate the adsorption behaviour of the chitosan/marble powder composites, 0.1 g adsorbent was added into 50 mL Diamozol Blue BRF %150 (C.I. Reactive Blue 221) solution with fixed concentrations (60 mg/L).
29168717	7	34	theme	Freundlich	1341:1350	arg1	models					1361:1366	the pseudo-second-order and Freundlich isotherm models	1313:1366	the pseudo-second-order and Freundlich isotherm models	1313:1366	The studies revealed that Dimozol Blue dye adsorption was described well by the pseudo-second-order and Freundlich isotherm models.
29168717	2	35	theme	scanning	406:413	arg1	SEM					436:438	SEM	436:438	SEM	436:438	The morphology and the surface area of the chitosan/marble powder composites were also characterized with a scanning electron microscope (SEM) and Micromeritics (ASAP 2020) BET (Brunauer, Emmett and Teller) instrument, respectively.
29168717	2	35	theme	scanning	406:413	arg1	microscope					424:433	a scanning electron microscope	404:433	a scanning electron microscope (SEM)	404:439	The morphology and the surface area of the chitosan/marble powder composites were also characterized with a scanning electron microscope (SEM) and Micromeritics (ASAP 2020) BET (Brunauer, Emmett and Teller) instrument, respectively.
29168717	3	36	theme	Reactive	676:683	arg1	221					690:692	C.I. Reactive Blue 221	671:692	C.I. Reactive Blue 221	671:692	To evaluate the adsorption behaviour of the chitosan/marble powder composites, 0.1 g adsorbent was added into 50 mL Diamozol Blue BRF %150 (C.I. Reactive Blue 221) solution with fixed concentrations (60 mg/L).
29168717	3	36	theme	Reactive	676:683	arg1	%					665:665	Diamozol Blue BRF %150	647:668	50 mL Diamozol Blue BRF %150 (C.I. Reactive Blue 221) solution with fixed concentrations (60 mg/L)	641:738	To evaluate the adsorption behaviour of the chitosan/marble powder composites, 0.1 g adsorbent was added into 50 mL Diamozol Blue BRF %150 (C.I. Reactive Blue 221) solution with fixed concentrations (60 mg/L).
29168717	2	37	dep	BET	471:473	arg1	instrument					505:514	instrument	505:514	instrument	505:514	The morphology and the surface area of the chitosan/marble powder composites were also characterized with a scanning electron microscope (SEM) and Micromeritics (ASAP 2020) BET (Brunauer, Emmett and Teller) instrument, respectively.
29168717	2	37	dep	BET	471:473	arg1	Teller					497:502	Teller	497:502	Teller	497:502	The morphology and the surface area of the chitosan/marble powder composites were also characterized with a scanning electron microscope (SEM) and Micromeritics (ASAP 2020) BET (Brunauer, Emmett and Teller) instrument, respectively.
29168717	2	37	dep	BET	471:473	arg1	Emmett					486:491	Emmett	486:491	Emmett	486:491	The morphology and the surface area of the chitosan/marble powder composites were also characterized with a scanning electron microscope (SEM) and Micromeritics (ASAP 2020) BET (Brunauer, Emmett and Teller) instrument, respectively.
29168717	1	38	theme	C	105:105	arg1	M					123:123	M	123:123	M	123:123	Cross-linked chitosan(C)/marble powder (M) composites with different weight ratio percentage (C100M0, C70M30, C50M50, and C30M70) were prepared from marble powder and chitosan and cross-linked using glutaraldehyde.
29168717	1	38	theme	C	105:105	arg1	powder					115:120	Cross-linked chitosan(C)/marble powder	83:120	Cross-linked chitosan(C)/marble powder (M) composites with different weight ratio percentage (C100M0, C70M30, C50M50, and C30M70)	83:211	Cross-linked chitosan(C)/marble powder (M) composites with different weight ratio percentage (C100M0, C70M30, C50M50, and C30M70) were prepared from marble powder and chitosan and cross-linked using glutaraldehyde.
29168717	7	39	theme	Blue	1271:1274	arg1	adsorption					1280:1289	Dimozol Blue dye adsorption	1263:1289	Dimozol Blue dye adsorption	1263:1289	The studies revealed that Dimozol Blue dye adsorption was described well by the pseudo-second-order and Freundlich isotherm models.
29168717	0	40	theme	powder	29:34	arg1	composites					36:45	chitosan/marble powder composites	13:45	chitosan/marble powder composites	13:45	Cross-linked chitosan/marble powder composites for the adsorption of Dimozol Blue.
29168717	8	41	theme	industrial	1477:1486	arg1	dyes					1488:1491	industrial dyes	1477:1491	industrial dyes	1477:1491	The results of this study indicated the applicability of the chitosan/marble powder composites for removing industrial dyes from aqueous solution.
29168717	5	42	from	solution	1077:1084	arg1	removal					1040:1046	the removal	1036:1046	the removal of Dimozol Blue from aqueous solution	1036:1084	C50M50 composite was more economical than C100M0 and C70M30 due to the higher marble powder content, and hence was selected as an adsorbent for the removal of Dimozol Blue from aqueous solution.
29168717	3	43	theme	composites	598:607	arg1	behaviour					558:566	the adsorption behaviour	543:566	the adsorption behaviour of the chitosan/marble powder composites	543:607	To evaluate the adsorption behaviour of the chitosan/marble powder composites, 0.1 g adsorbent was added into 50 mL Diamozol Blue BRF %150 (C.I. Reactive Blue 221) solution with fixed concentrations (60 mg/L).
29168717	8	44	theme	powder	1446:1451	arg1	composites					1453:1462	the chitosan/marble powder composites	1426:1462	the chitosan/marble powder composites	1426:1462	The results of this study indicated the applicability of the chitosan/marble powder composites for removing industrial dyes from aqueous solution.
29168717	5	45	theme	Dimozol	1051:1057	arg1	removal					1040:1046	the removal	1036:1046	the removal of Dimozol Blue from aqueous solution	1036:1084	C50M50 composite was more economical than C100M0 and C70M30 due to the higher marble powder content, and hence was selected as an adsorbent for the removal of Dimozol Blue from aqueous solution.
29168717	1	46	dep	percentage	165:174	arg1	C50M50					193:198	C50M50	193:198	C50M50	193:198	Cross-linked chitosan(C)/marble powder (M) composites with different weight ratio percentage (C100M0, C70M30, C50M50, and C30M70) were prepared from marble powder and chitosan and cross-linked using glutaraldehyde.
29168717	1	46	dep	percentage	165:174	arg1	C30M70					205:210	C30M70	205:210	C30M70	205:210	Cross-linked chitosan(C)/marble powder (M) composites with different weight ratio percentage (C100M0, C70M30, C50M50, and C30M70) were prepared from marble powder and chitosan and cross-linked using glutaraldehyde.
29168717	1	46	dep	percentage	165:174	arg1	C70M30					185:190	C70M30	185:190	C70M30	185:190	Cross-linked chitosan(C)/marble powder (M) composites with different weight ratio percentage (C100M0, C70M30, C50M50, and C30M70) were prepared from marble powder and chitosan and cross-linked using glutaraldehyde.
29168717	2	47	theme	composites	364:373	arg1	morphology					302:311	The morphology	298:311	The morphology	298:311	The morphology and the surface area of the chitosan/marble powder composites were also characterized with a scanning electron microscope (SEM) and Micromeritics (ASAP 2020) BET (Brunauer, Emmett and Teller) instrument, respectively.
29168717	2	47	theme	composites	364:373	arg1	area					329:332	the surface area	317:332	the surface area of the chitosan/marble powder composites	317:373	The morphology and the surface area of the chitosan/marble powder composites were also characterized with a scanning electron microscope (SEM) and Micromeritics (ASAP 2020) BET (Brunauer, Emmett and Teller) instrument, respectively.
29168717	4	48	theme	Blue	822:825	arg1	Dimozol					814:820	Dimozol Blue	814:825	Dimozol Blue	814:825	At equilibrium, the adsorption capacity of C100M0, C70M30 and C50M50 for Dimozol Blue was about 27 mg/g and significantly greater than that of C30M70.
29168717	3	49	theme	chitosan/marble	575:589	arg1	composites					598:607	the chitosan/marble powder composites	571:607	the chitosan/marble powder composites	571:607	To evaluate the adsorption behaviour of the chitosan/marble powder composites, 0.1 g adsorbent was added into 50 mL Diamozol Blue BRF %150 (C.I. Reactive Blue 221) solution with fixed concentrations (60 mg/L).
29168717	6	50	theme	adsorption	1091:1100	arg1	kinetics					1102:1109	adsorption kinetics	1091:1109	adsorption kinetics	1091:1109	The adsorption kinetics and equilibrium isotherms of Dimozol Blue onto the chitosan/marble powder composites from aqueous solution were investigated.
29168717	8	51	theme	chitosan/marble	1430:1444	arg1	composites					1453:1462	the chitosan/marble powder composites	1426:1462	the chitosan/marble powder composites	1426:1462	The results of this study indicated the applicability of the chitosan/marble powder composites for removing industrial dyes from aqueous solution.
29168717	4	52	theme	C70M30	792:797	arg1	mg/g					840:843	about 27 mg/g	831:843	about 27 mg/g	831:843	At equilibrium, the adsorption capacity of C100M0, C70M30 and C50M50 for Dimozol Blue was about 27 mg/g and significantly greater than that of C30M70.
29168717	4	52	theme	C70M30	792:797	arg1	capacity					772:779	the adsorption capacity	757:779	the adsorption capacity of C100M0, C70M30 and C50M50 for Dimozol Blue	757:825	At equilibrium, the adsorption capacity of C100M0, C70M30 and C50M50 for Dimozol Blue was about 27 mg/g and significantly greater than that of C30M70.
29168717	0	53	theme	Dimozol	69:75	arg1	adsorption					55:64	the adsorption	51:64	the adsorption of Dimozol Blue	51:80	Cross-linked chitosan/marble powder composites for the adsorption of Dimozol Blue.
29168717	4	54	from	equilibrium	744:754	arg1	mg/g					840:843	about 27 mg/g	831:843	about 27 mg/g	831:843	At equilibrium, the adsorption capacity of C100M0, C70M30 and C50M50 for Dimozol Blue was about 27 mg/g and significantly greater than that of C30M70.
29168717	4	54	from	equilibrium	744:754	arg1	capacity					772:779	the adsorption capacity	757:779	the adsorption capacity of C100M0, C70M30 and C50M50 for Dimozol Blue	757:825	At equilibrium, the adsorption capacity of C100M0, C70M30 and C50M50 for Dimozol Blue was about 27 mg/g and significantly greater than that of C30M70.
29168717	1	55	theme	/marble	107:113	arg1	M					123:123	M	123:123	M	123:123	Cross-linked chitosan(C)/marble powder (M) composites with different weight ratio percentage (C100M0, C70M30, C50M50, and C30M70) were prepared from marble powder and chitosan and cross-linked using glutaraldehyde.
29168717	1	55	theme	/marble	107:113	arg1	powder					115:120	Cross-linked chitosan(C)/marble powder	83:120	Cross-linked chitosan(C)/marble powder (M) composites with different weight ratio percentage (C100M0, C70M30, C50M50, and C30M70)	83:211	Cross-linked chitosan(C)/marble powder (M) composites with different weight ratio percentage (C100M0, C70M30, C50M50, and C30M70) were prepared from marble powder and chitosan and cross-linked using glutaraldehyde.
29168717	6	56	theme	aqueous	1201:1207	arg1	solution					1209:1216	aqueous solution	1201:1216	aqueous solution	1201:1216	The adsorption kinetics and equilibrium isotherms of Dimozol Blue onto the chitosan/marble powder composites from aqueous solution were investigated.
29168717	3	57	theme	Blue	685:688	arg1	221					690:692	C.I. Reactive Blue 221	671:692	C.I. Reactive Blue 221	671:692	To evaluate the adsorption behaviour of the chitosan/marble powder composites, 0.1 g adsorbent was added into 50 mL Diamozol Blue BRF %150 (C.I. Reactive Blue 221) solution with fixed concentrations (60 mg/L).
29168717	3	57	theme	Blue	685:688	arg1	%					665:665	Diamozol Blue BRF %150	647:668	50 mL Diamozol Blue BRF %150 (C.I. Reactive Blue 221) solution with fixed concentrations (60 mg/L)	641:738	To evaluate the adsorption behaviour of the chitosan/marble powder composites, 0.1 g adsorbent was added into 50 mL Diamozol Blue BRF %150 (C.I. Reactive Blue 221) solution with fixed concentrations (60 mg/L).
29168717	3	58	theme	powder	591:596	arg1	composites					598:607	the chitosan/marble powder composites	571:607	the chitosan/marble powder composites	571:607	To evaluate the adsorption behaviour of the chitosan/marble powder composites, 0.1 g adsorbent was added into 50 mL Diamozol Blue BRF %150 (C.I. Reactive Blue 221) solution with fixed concentrations (60 mg/L).
29168717	7	59	theme	Dimozol	1263:1269	arg1	adsorption					1280:1289	Dimozol Blue dye adsorption	1263:1289	Dimozol Blue dye adsorption	1263:1289	The studies revealed that Dimozol Blue dye adsorption was described well by the pseudo-second-order and Freundlich isotherm models.
29168717	8	60	attach	removing	1468:1475	arg2	applicability					1409:1421	the applicability	1405:1421	the applicability of the chitosan/marble powder composites for removing industrial dyes from aqueous solution	1405:1513	The results of this study indicated the applicability of the chitosan/marble powder composites for removing industrial dyes from aqueous solution.
29168717	8	60	attach	removing	1468:1475	arg1	solution					1506:1513	aqueous solution	1498:1513	aqueous solution	1498:1513	The results of this study indicated the applicability of the chitosan/marble powder composites for removing industrial dyes from aqueous solution.
29168717	1	61	theme	powder	115:120	arg1	composites					126:135	Cross-linked chitosan(C)/marble powder (M) composites	83:135	Cross-linked chitosan(C)/marble powder (M) composites with different weight ratio percentage (C100M0, C70M30, C50M50, and C30M70)	83:211	Cross-linked chitosan(C)/marble powder (M) composites with different weight ratio percentage (C100M0, C70M30, C50M50, and C30M70) were prepared from marble powder and chitosan and cross-linked using glutaraldehyde.
29168717	8	62	theme	aqueous	1498:1504	arg1	solution					1506:1513	aqueous solution	1498:1513	aqueous solution	1498:1513	The results of this study indicated the applicability of the chitosan/marble powder composites for removing industrial dyes from aqueous solution.
29168717	4	63	theme	C50M50	803:808	arg1	mg/g					840:843	about 27 mg/g	831:843	about 27 mg/g	831:843	At equilibrium, the adsorption capacity of C100M0, C70M30 and C50M50 for Dimozol Blue was about 27 mg/g and significantly greater than that of C30M70.
29168717	4	63	theme	C50M50	803:808	arg1	capacity					772:779	the adsorption capacity	757:779	the adsorption capacity of C100M0, C70M30 and C50M50 for Dimozol Blue	757:825	At equilibrium, the adsorption capacity of C100M0, C70M30 and C50M50 for Dimozol Blue was about 27 mg/g and significantly greater than that of C30M70.
29168717	6	64	from	solution	1209:1216	arg1	composites					1185:1194	the chitosan/marble powder composites	1158:1194	the chitosan/marble powder composites from aqueous solution	1158:1216	The adsorption kinetics and equilibrium isotherms of Dimozol Blue onto the chitosan/marble powder composites from aqueous solution were investigated.
29168717	6	65	theme	equilibrium	1115:1125	arg1	isotherms					1127:1135	equilibrium isotherms	1115:1135	equilibrium isotherms	1115:1135	The adsorption kinetics and equilibrium isotherms of Dimozol Blue onto the chitosan/marble powder composites from aqueous solution were investigated.
29168717	6	66	theme	powder	1178:1183	arg1	composites					1185:1194	the chitosan/marble powder composites	1158:1194	the chitosan/marble powder composites from aqueous solution	1158:1216	The adsorption kinetics and equilibrium isotherms of Dimozol Blue onto the chitosan/marble powder composites from aqueous solution were investigated.
29168717	6	67	theme	chitosan/marble	1162:1176	arg1	composites					1185:1194	the chitosan/marble powder composites	1158:1194	the chitosan/marble powder composites from aqueous solution	1158:1216	The adsorption kinetics and equilibrium isotherms of Dimozol Blue onto the chitosan/marble powder composites from aqueous solution were investigated.
29168717	0	68	theme	Blue	77:80	arg1	Dimozol					69:75	Dimozol Blue	69:80	Dimozol Blue	69:80	Cross-linked chitosan/marble powder composites for the adsorption of Dimozol Blue.
29168717	3	69	theme	adsorption	547:556	arg1	behaviour					558:566	the adsorption behaviour	543:566	the adsorption behaviour of the chitosan/marble powder composites	543:607	To evaluate the adsorption behaviour of the chitosan/marble powder composites, 0.1 g adsorbent was added into 50 mL Diamozol Blue BRF %150 (C.I. Reactive Blue 221) solution with fixed concentrations (60 mg/L).
29168717	8	70	theme	composites	1453:1462	arg1	applicability					1409:1421	the applicability	1405:1421	the applicability of the chitosan/marble powder composites for removing industrial dyes from aqueous solution	1405:1513	The results of this study indicated the applicability of the chitosan/marble powder composites for removing industrial dyes from aqueous solution.
29168717	2	71	theme	powder	357:362	arg1	composites					364:373	the chitosan/marble powder composites	337:373	the chitosan/marble powder composites	337:373	The morphology and the surface area of the chitosan/marble powder composites were also characterized with a scanning electron microscope (SEM) and Micromeritics (ASAP 2020) BET (Brunauer, Emmett and Teller) instrument, respectively.
29168717	2	72	theme	ASAP	460:463	arg1	Micromeritics					445:457	Micromeritics	445:457	Micromeritics (ASAP 2020)	445:469	The morphology and the surface area of the chitosan/marble powder composites were also characterized with a scanning electron microscope (SEM) and Micromeritics (ASAP 2020) BET (Brunauer, Emmett and Teller) instrument, respectively.
29168717	2	72	theme	ASAP	460:463	arg1	2020					465:468	ASAP 2020	460:468	ASAP 2020	460:468	The morphology and the surface area of the chitosan/marble powder composites were also characterized with a scanning electron microscope (SEM) and Micromeritics (ASAP 2020) BET (Brunauer, Emmett and Teller) instrument, respectively.
29168717	3	73	theme	50	641:642	arg1	mL					644:645	mL	644:645	mL	644:645	To evaluate the adsorption behaviour of the chitosan/marble powder composites, 0.1 g adsorbent was added into 50 mL Diamozol Blue BRF %150 (C.I. Reactive Blue 221) solution with fixed concentrations (60 mg/L).
29168717	5	74	theme	C50M50	892:897	arg1	adsorbent					1022:1030	adsorbent	1022:1030	adsorbent	1022:1030	C50M50 composite was more economical than C100M0 and C70M30 due to the higher marble powder content, and hence was selected as an adsorbent for the removal of Dimozol Blue from aqueous solution.
29168717	5	74	theme	C50M50	892:897	arg1	composite					899:907	C50M50 composite	892:907	C50M50 composite	892:907	C50M50 composite was more economical than C100M0 and C70M30 due to the higher marble powder content, and hence was selected as an adsorbent for the removal of Dimozol Blue from aqueous solution.
29168717	6	75	theme	Blue	1148:1151	arg1	Dimozol					1140:1146	Dimozol Blue	1140:1151	Dimozol Blue onto the chitosan/marble powder composites from aqueous solution	1140:1216	The adsorption kinetics and equilibrium isotherms of Dimozol Blue onto the chitosan/marble powder composites from aqueous solution were investigated.
29168717	3	76	theme	fixed	709:713	arg1	concentrations					715:728	fixed concentrations	709:728	fixed concentrations (60 mg/L)	709:738	To evaluate the adsorption behaviour of the chitosan/marble powder composites, 0.1 g adsorbent was added into 50 mL Diamozol Blue BRF %150 (C.I. Reactive Blue 221) solution with fixed concentrations (60 mg/L).
29168717	3	76	theme	fixed	709:713	arg1	mg/L					734:737	60 mg/L	731:737	60 mg/L	731:737	To evaluate the adsorption behaviour of the chitosan/marble powder composites, 0.1 g adsorbent was added into 50 mL Diamozol Blue BRF %150 (C.I. Reactive Blue 221) solution with fixed concentrations (60 mg/L).
27999373	6	0	theme	polar	976:980	arg1	solvents					990:997	polar organic solvents	976:997	polar organic solvents	976:997	The precipitation behavior of galactomannans in polar organic solvents was dependent on the molecular structures and properties of the solvent.
27999373	2	1	theme	ethanol	342:348	arg1	concentrations					324:337	different concentrations	314:337	different concentrations of ethanol and isopropanol	314:364	G. sinensis gum (GSG) and G. microphylla gum (GMG) were purified and precipitated using different concentrations of ethanol and isopropanol.
27999373	4	2	theme	galactomannan	582:594	arg1	recovery					596:603	The galactomannan recovery	578:603	The galactomannan recovery of GSG and GMG in 33.3% ethanol	578:635	The galactomannan recovery of GSG and GMG in 33.3% ethanol was 81.7% and 82.5%, respectively, while that in 28.8% isopropanol was 81.3% and 82.9%, respectively.
27999373	4	2	theme	galactomannan	582:594	arg1	%					645:645	81.7%	641:645	81.7%	641:645	The galactomannan recovery of GSG and GMG in 33.3% ethanol was 81.7% and 82.5%, respectively, while that in 28.8% isopropanol was 81.3% and 82.9%, respectively.
27999373	5	3	theme	isopropanol	891:901	arg1	constant					879:886	the lower dielectric constant	858:886	the lower dielectric constant of isopropanol	858:901	To achieve similar precipitation efficiency, the amount of isopropanol should be lower than that of ethanol because of the lower dielectric constant of isopropanol (20 vs. 25 for ethanol).
27999373	7	4	theme	alcohols	1176:1183	arg1	concentration					1159:1171	a lower concentration	1151:1171	a lower concentration of alcohols	1151:1183	A higher mannose/galactose ratio and a higher molecular weight was obtained in a lower concentration of alcohols.
27999373	6	5	theme	molecular	1020:1028	arg1	structures					1030:1039	molecular structures	1020:1039	molecular structures	1020:1039	The precipitation behavior of galactomannans in polar organic solvents was dependent on the molecular structures and properties of the solvent.
27999373	0	6	from	Characterization	0:15	arg1	Gums					97:100	Gleditsia microphylla Gums	75:100	Gleditsia microphylla Gums	75:100	Characterization of Fractional Polysaccharides from Gleditsia sinensis and Gleditsia microphylla Gums.
27999373	0	6	from	Characterization	0:15	arg1	sinensis					62:69	Gleditsia sinensis	52:69	Gleditsia sinensis	52:69	Characterization of Fractional Polysaccharides from Gleditsia sinensis and Gleditsia microphylla Gums.
27999373	3	7	dep	GSG	371:373	arg1	The					367:369	The	367:369	The	367:369	The GSG and GMG, precipitated in different stages, presented different characteristics, including polymer recovery, mannose/galactose ratio, chemical composition, molecular weight, and morphological appearance.
27999373	1	8	from	China	176:180	arg1	widespread					162:171	widespread	162:171	widespread	162:171	The seeds of Gleditsia sinensis and Gleditsia microphylla, widespread in China, are an important source of galactomannans.
27999373	3	9	theme	morphological	552:564	arg1	appearance					566:575	morphological appearance	552:575	morphological appearance	552:575	The GSG and GMG, precipitated in different stages, presented different characteristics, including polymer recovery, mannose/galactose ratio, chemical composition, molecular weight, and morphological appearance.
27999373	4	10	from	recovery	596:603	arg1	ethanol					629:635	33.3% ethanol	623:635	33.3% ethanol	623:635	The galactomannan recovery of GSG and GMG in 33.3% ethanol was 81.7% and 82.5%, respectively, while that in 28.8% isopropanol was 81.3% and 82.9%, respectively.
27999373	4	11	theme	33.3	623:626	arg1	%					627:627	%	627:627	%	627:627	The galactomannan recovery of GSG and GMG in 33.3% ethanol was 81.7% and 82.5%, respectively, while that in 28.8% isopropanol was 81.3% and 82.9%, respectively.
27999373	6	12	from	behavior	946:953	arg1	solvents					990:997	polar organic solvents	976:997	polar organic solvents	976:997	The precipitation behavior of galactomannans in polar organic solvents was dependent on the molecular structures and properties of the solvent.
27999373	4	13	theme	28.8	686:689	arg1	%					690:690	%	690:690	%	690:690	The galactomannan recovery of GSG and GMG in 33.3% ethanol was 81.7% and 82.5%, respectively, while that in 28.8% isopropanol was 81.3% and 82.9%, respectively.
27999373	5	14	theme	lower	862:866	arg1	constant					879:886	the lower dielectric constant	858:886	the lower dielectric constant of isopropanol	858:901	To achieve similar precipitation efficiency, the amount of isopropanol should be lower than that of ethanol because of the lower dielectric constant of isopropanol (20 vs. 25 for ethanol).
27999373	7	15	theme	molecular	1118:1126	arg1	weight					1128:1133	a higher molecular weight	1109:1133	a higher molecular weight	1109:1133	A higher mannose/galactose ratio and a higher molecular weight was obtained in a lower concentration of alcohols.
27999373	0	16	from	sinensis	62:69	arg1	Characterization					0:15	Characterization	0:15	Characterization of Fractional Polysaccharides from Gleditsia sinensis and Gleditsia microphylla Gums.	0:101	Characterization of Fractional Polysaccharides from Gleditsia sinensis and Gleditsia microphylla Gums.
27999373	0	16	from	sinensis	62:69	arg1	Polysaccharides					31:45	Fractional Polysaccharides	20:45	Fractional Polysaccharides from Gleditsia sinensis and Gleditsia microphylla Gums	20:100	Characterization of Fractional Polysaccharides from Gleditsia sinensis and Gleditsia microphylla Gums.
27999373	1	17	from	widespread	162:171	arg1	China					176:180	China	176:180	China	176:180	The seeds of Gleditsia sinensis and Gleditsia microphylla, widespread in China, are an important source of galactomannans.
27999373	5	18	theme	isopropanol	798:808	arg1	amount					788:793	the amount	784:793	the amount of isopropanol	784:808	To achieve similar precipitation efficiency, the amount of isopropanol should be lower than that of ethanol because of the lower dielectric constant of isopropanol (20 vs. 25 for ethanol).
27999373	5	18	theme	isopropanol	798:808	arg1	isopropanol					798:808	isopropanol	798:808	isopropanol	798:808	To achieve similar precipitation efficiency, the amount of isopropanol should be lower than that of ethanol because of the lower dielectric constant of isopropanol (20 vs. 25 for ethanol).
27999373	5	18	theme	isopropanol	798:808	arg1	lower					820:824	lower	820:824	lower	820:824	To achieve similar precipitation efficiency, the amount of isopropanol should be lower than that of ethanol because of the lower dielectric constant of isopropanol (20 vs. 25 for ethanol).
27999373	7	19	theme	higher	1074:1079	arg1	ratio					1099:1103	A higher mannose/galactose ratio	1072:1103	A higher mannose/galactose ratio	1072:1103	A higher mannose/galactose ratio and a higher molecular weight was obtained in a lower concentration of alcohols.
27999373	2	20	theme	microphylla	255:265	arg1	GMG					272:274	GMG	272:274	GMG	272:274	G. sinensis gum (GSG) and G. microphylla gum (GMG) were purified and precipitated using different concentrations of ethanol and isopropanol.
27999373	2	20	theme	microphylla	255:265	arg1	gum					267:269	G. microphylla gum	252:269	G. microphylla gum (GMG)	252:275	G. sinensis gum (GSG) and G. microphylla gum (GMG) were purified and precipitated using different concentrations of ethanol and isopropanol.
27999373	7	21	theme	lower	1153:1157	arg1	concentration					1159:1171	a lower concentration	1151:1171	a lower concentration of alcohols	1151:1183	A higher mannose/galactose ratio and a higher molecular weight was obtained in a lower concentration of alcohols.
27999373	5	22	theme	dielectric	868:877	arg1	constant					879:886	the lower dielectric constant	858:886	the lower dielectric constant of isopropanol	858:901	To achieve similar precipitation efficiency, the amount of isopropanol should be lower than that of ethanol because of the lower dielectric constant of isopropanol (20 vs. 25 for ethanol).
27999373	3	23	theme	chemical	508:515	arg1	composition					517:527	chemical composition	508:527	chemical composition	508:527	The GSG and GMG, precipitated in different stages, presented different characteristics, including polymer recovery, mannose/galactose ratio, chemical composition, molecular weight, and morphological appearance.
27999373	3	24	theme	different	400:408	arg1	stages					410:415	different stages	400:415	different stages	400:415	The GSG and GMG, precipitated in different stages, presented different characteristics, including polymer recovery, mannose/galactose ratio, chemical composition, molecular weight, and morphological appearance.
27999373	5	25	theme	similar	750:756	arg1	efficiency					772:781	similar precipitation efficiency	750:781	similar precipitation efficiency	750:781	To achieve similar precipitation efficiency, the amount of isopropanol should be lower than that of ethanol because of the lower dielectric constant of isopropanol (20 vs. 25 for ethanol).
27999373	4	26	theme	%	690:690	arg1	isopropanol					692:702	28.8% isopropanol	686:702	28.8% isopropanol	686:702	The galactomannan recovery of GSG and GMG in 33.3% ethanol was 81.7% and 82.5%, respectively, while that in 28.8% isopropanol was 81.3% and 82.9%, respectively.
27999373	1	27	theme	important	190:198	arg1	seeds					107:111	The seeds	103:111	The seeds	103:111	The seeds of Gleditsia sinensis and Gleditsia microphylla, widespread in China, are an important source of galactomannans.
27999373	1	27	theme	important	190:198	arg1	source					200:205	an important source	187:205	an important source of galactomannans	187:223	The seeds of Gleditsia sinensis and Gleditsia microphylla, widespread in China, are an important source of galactomannans.
27999373	0	28	theme	Polysaccharides	31:45	arg1	Characterization					0:15	Characterization	0:15	Characterization of Fractional Polysaccharides from Gleditsia sinensis and Gleditsia microphylla Gums.	0:101	Characterization of Fractional Polysaccharides from Gleditsia sinensis and Gleditsia microphylla Gums.
27999373	6	29	dep	structures	1030:1039	arg1	the					1016:1018	the	1016:1018	the	1016:1018	The precipitation behavior of galactomannans in polar organic solvents was dependent on the molecular structures and properties of the solvent.
27999373	4	30	theme	%	627:627	arg1	ethanol					629:635	33.3% ethanol	623:635	33.3% ethanol	623:635	The galactomannan recovery of GSG and GMG in 33.3% ethanol was 81.7% and 82.5%, respectively, while that in 28.8% isopropanol was 81.3% and 82.9%, respectively.
27999373	0	31	theme	Fractional	20:29	arg1	Polysaccharides					31:45	Fractional Polysaccharides	20:45	Fractional Polysaccharides from Gleditsia sinensis and Gleditsia microphylla Gums	20:100	Characterization of Fractional Polysaccharides from Gleditsia sinensis and Gleditsia microphylla Gums.
27999373	1	32	theme	Gleditsia	116:124	arg1	sinensis					126:133	Gleditsia sinensis	116:133	Gleditsia sinensis	116:133	The seeds of Gleditsia sinensis and Gleditsia microphylla, widespread in China, are an important source of galactomannans.
27999373	2	33	dep	G.	226:227	arg1	sinensis					229:236	sinensis	229:236	sinensis	229:236	G. sinensis gum (GSG) and G. microphylla gum (GMG) were purified and precipitated using different concentrations of ethanol and isopropanol.
27999373	0	34	theme	Gleditsia	52:60	arg1	sinensis					62:69	Gleditsia sinensis	52:69	Gleditsia sinensis	52:69	Characterization of Fractional Polysaccharides from Gleditsia sinensis and Gleditsia microphylla Gums.
27999373	3	35	theme	mannose/galactose	483:499	arg1	ratio					501:505	mannose/galactose ratio	483:505	mannose/galactose ratio	483:505	The GSG and GMG, precipitated in different stages, presented different characteristics, including polymer recovery, mannose/galactose ratio, chemical composition, molecular weight, and morphological appearance.
27999373	0	36	from	Gums	97:100	arg1	Characterization					0:15	Characterization	0:15	Characterization of Fractional Polysaccharides from Gleditsia sinensis and Gleditsia microphylla Gums.	0:101	Characterization of Fractional Polysaccharides from Gleditsia sinensis and Gleditsia microphylla Gums.
27999373	0	36	from	Gums	97:100	arg1	Polysaccharides					31:45	Fractional Polysaccharides	20:45	Fractional Polysaccharides from Gleditsia sinensis and Gleditsia microphylla Gums	20:100	Characterization of Fractional Polysaccharides from Gleditsia sinensis and Gleditsia microphylla Gums.
27999373	1	37	theme	galactomannans	210:223	arg1	seeds					107:111	The seeds	103:111	The seeds	103:111	The seeds of Gleditsia sinensis and Gleditsia microphylla, widespread in China, are an important source of galactomannans.
27999373	1	37	theme	galactomannans	210:223	arg1	source					200:205	an important source	187:205	an important source of galactomannans	187:223	The seeds of Gleditsia sinensis and Gleditsia microphylla, widespread in China, are an important source of galactomannans.
27999373	7	38	theme	mannose/galactose	1081:1097	arg1	ratio					1099:1103	A higher mannose/galactose ratio	1072:1103	A higher mannose/galactose ratio	1072:1103	A higher mannose/galactose ratio and a higher molecular weight was obtained in a lower concentration of alcohols.
27999373	5	39	theme	precipitation	758:770	arg1	efficiency					772:781	similar precipitation efficiency	750:781	similar precipitation efficiency	750:781	To achieve similar precipitation efficiency, the amount of isopropanol should be lower than that of ethanol because of the lower dielectric constant of isopropanol (20 vs. 25 for ethanol).
27999373	5	40	dep	ethanol	918:924	arg1	20					904:905	20	904:905	20	904:905	To achieve similar precipitation efficiency, the amount of isopropanol should be lower than that of ethanol because of the lower dielectric constant of isopropanol (20 vs. 25 for ethanol).
27999373	5	40	dep	ethanol	918:924	arg1	25					911:912	25	911:912	25	911:912	To achieve similar precipitation efficiency, the amount of isopropanol should be lower than that of ethanol because of the lower dielectric constant of isopropanol (20 vs. 25 for ethanol).
27999373	1	41	theme	sinensis	126:133	arg1	seeds					107:111	The seeds	103:111	The seeds	103:111	The seeds of Gleditsia sinensis and Gleditsia microphylla, widespread in China, are an important source of galactomannans.
27999373	1	41	theme	sinensis	126:133	arg1	source					200:205	an important source	187:205	an important source of galactomannans	187:223	The seeds of Gleditsia sinensis and Gleditsia microphylla, widespread in China, are an important source of galactomannans.
27999373	4	42	theme	GSG	608:610	arg1	recovery					596:603	The galactomannan recovery	578:603	The galactomannan recovery of GSG and GMG in 33.3% ethanol	578:635	The galactomannan recovery of GSG and GMG in 33.3% ethanol was 81.7% and 82.5%, respectively, while that in 28.8% isopropanol was 81.3% and 82.9%, respectively.
27999373	4	42	theme	GSG	608:610	arg1	%					645:645	81.7%	641:645	81.7%	641:645	The galactomannan recovery of GSG and GMG in 33.3% ethanol was 81.7% and 82.5%, respectively, while that in 28.8% isopropanol was 81.3% and 82.9%, respectively.
27999373	2	43	theme	G.	226:227	arg1	gum					238:240	G. sinensis gum	226:240	G. sinensis gum (GSG)	226:246	G. sinensis gum (GSG) and G. microphylla gum (GMG) were purified and precipitated using different concentrations of ethanol and isopropanol.
27999373	2	43	theme	G.	226:227	arg1	GSG					243:245	GSG	243:245	GSG	243:245	G. sinensis gum (GSG) and G. microphylla gum (GMG) were purified and precipitated using different concentrations of ethanol and isopropanol.
27999373	2	44	theme	different	314:322	arg1	concentrations					324:337	different concentrations	314:337	different concentrations of ethanol and isopropanol	314:364	G. sinensis gum (GSG) and G. microphylla gum (GMG) were purified and precipitated using different concentrations of ethanol and isopropanol.
27999373	2	45	theme	G.	252:253	arg1	GMG					272:274	GMG	272:274	GMG	272:274	G. sinensis gum (GSG) and G. microphylla gum (GMG) were purified and precipitated using different concentrations of ethanol and isopropanol.
27999373	2	45	theme	G.	252:253	arg1	gum					267:269	G. microphylla gum	252:269	G. microphylla gum (GMG)	252:275	G. sinensis gum (GSG) and G. microphylla gum (GMG) were purified and precipitated using different concentrations of ethanol and isopropanol.
27999373	5	46	dep	lower	820:824	arg1	ethanol					918:924	ethanol	918:924	ethanol	918:924	To achieve similar precipitation efficiency, the amount of isopropanol should be lower than that of ethanol because of the lower dielectric constant of isopropanol (20 vs. 25 for ethanol).
27999373	1	47	theme	Gleditsia	139:147	arg1	microphylla					149:159	Gleditsia microphylla	139:159	Gleditsia microphylla	139:159	The seeds of Gleditsia sinensis and Gleditsia microphylla, widespread in China, are an important source of galactomannans.
27999373	3	48	theme	molecular	530:538	arg1	weight					540:545	molecular weight	530:545	molecular weight	530:545	The GSG and GMG, precipitated in different stages, presented different characteristics, including polymer recovery, mannose/galactose ratio, chemical composition, molecular weight, and morphological appearance.
27999373	1	49	theme	microphylla	149:159	arg1	seeds					107:111	The seeds	103:111	The seeds	103:111	The seeds of Gleditsia sinensis and Gleditsia microphylla, widespread in China, are an important source of galactomannans.
27999373	1	49	theme	microphylla	149:159	arg1	source					200:205	an important source	187:205	an important source of galactomannans	187:223	The seeds of Gleditsia sinensis and Gleditsia microphylla, widespread in China, are an important source of galactomannans.
27999373	6	50	theme	solvent	1063:1069	arg1	structures					1030:1039	molecular structures	1020:1039	molecular structures	1020:1039	The precipitation behavior of galactomannans in polar organic solvents was dependent on the molecular structures and properties of the solvent.
27999373	6	50	theme	solvent	1063:1069	arg1	properties					1045:1054	properties	1045:1054	properties	1045:1054	The precipitation behavior of galactomannans in polar organic solvents was dependent on the molecular structures and properties of the solvent.
27999373	3	51	theme	polymer	465:471	arg1	recovery					473:480	polymer recovery	465:480	polymer recovery	465:480	The GSG and GMG, precipitated in different stages, presented different characteristics, including polymer recovery, mannose/galactose ratio, chemical composition, molecular weight, and morphological appearance.
27999373	0	52	theme	microphylla	85:95	arg1	Gums					97:100	Gleditsia microphylla Gums	75:100	Gleditsia microphylla Gums	75:100	Characterization of Fractional Polysaccharides from Gleditsia sinensis and Gleditsia microphylla Gums.
27999373	6	53	theme	galactomannans	958:971	arg1	dependent					1003:1011	dependent	1003:1011	dependent	1003:1011	The precipitation behavior of galactomannans in polar organic solvents was dependent on the molecular structures and properties of the solvent.
27999373	6	53	theme	galactomannans	958:971	arg1	behavior					946:953	The precipitation behavior	928:953	The precipitation behavior of galactomannans in polar organic solvents	928:997	The precipitation behavior of galactomannans in polar organic solvents was dependent on the molecular structures and properties of the solvent.
27999373	3	54	theme	different	428:436	arg1	composition					517:527	chemical composition	508:527	chemical composition	508:527	The GSG and GMG, precipitated in different stages, presented different characteristics, including polymer recovery, mannose/galactose ratio, chemical composition, molecular weight, and morphological appearance.
27999373	3	54	theme	different	428:436	arg1	appearance					566:575	morphological appearance	552:575	morphological appearance	552:575	The GSG and GMG, precipitated in different stages, presented different characteristics, including polymer recovery, mannose/galactose ratio, chemical composition, molecular weight, and morphological appearance.
27999373	3	54	theme	different	428:436	arg1	recovery					473:480	polymer recovery	465:480	polymer recovery	465:480	The GSG and GMG, precipitated in different stages, presented different characteristics, including polymer recovery, mannose/galactose ratio, chemical composition, molecular weight, and morphological appearance.
27999373	3	54	theme	different	428:436	arg1	weight					540:545	molecular weight	530:545	molecular weight	530:545	The GSG and GMG, precipitated in different stages, presented different characteristics, including polymer recovery, mannose/galactose ratio, chemical composition, molecular weight, and morphological appearance.
27999373	3	54	theme	different	428:436	arg1	characteristics					438:452	different characteristics	428:452	different characteristics	428:452	The GSG and GMG, precipitated in different stages, presented different characteristics, including polymer recovery, mannose/galactose ratio, chemical composition, molecular weight, and morphological appearance.
27999373	3	54	theme	different	428:436	arg1	ratio					501:505	mannose/galactose ratio	483:505	mannose/galactose ratio	483:505	The GSG and GMG, precipitated in different stages, presented different characteristics, including polymer recovery, mannose/galactose ratio, chemical composition, molecular weight, and morphological appearance.
27999373	6	55	theme	organic	982:988	arg1	solvents					990:997	polar organic solvents	976:997	polar organic solvents	976:997	The precipitation behavior of galactomannans in polar organic solvents was dependent on the molecular structures and properties of the solvent.
27999373	2	56	theme	isopropanol	354:364	arg1	concentrations					324:337	different concentrations	314:337	different concentrations of ethanol and isopropanol	314:364	G. sinensis gum (GSG) and G. microphylla gum (GMG) were purified and precipitated using different concentrations of ethanol and isopropanol.
27999373	6	57	theme	precipitation	932:944	arg1	dependent					1003:1011	dependent	1003:1011	dependent	1003:1011	The precipitation behavior of galactomannans in polar organic solvents was dependent on the molecular structures and properties of the solvent.
27999373	6	57	theme	precipitation	932:944	arg1	behavior					946:953	The precipitation behavior	928:953	The precipitation behavior of galactomannans in polar organic solvents	928:997	The precipitation behavior of galactomannans in polar organic solvents was dependent on the molecular structures and properties of the solvent.
27999373	0	58	theme	Gleditsia	75:83	arg1	Gums					97:100	Gleditsia microphylla Gums	75:100	Gleditsia microphylla Gums	75:100	Characterization of Fractional Polysaccharides from Gleditsia sinensis and Gleditsia microphylla Gums.
27999373	1	59	theme	widespread	162:171	arg1	seeds					107:111	The seeds	103:111	The seeds	103:111	The seeds of Gleditsia sinensis and Gleditsia microphylla, widespread in China, are an important source of galactomannans.
27999373	1	59	theme	widespread	162:171	arg1	source					200:205	an important source	187:205	an important source of galactomannans	187:223	The seeds of Gleditsia sinensis and Gleditsia microphylla, widespread in China, are an important source of galactomannans.
27999373	7	60	theme	higher	1111:1116	arg1	weight					1128:1133	a higher molecular weight	1109:1133	a higher molecular weight	1109:1133	A higher mannose/galactose ratio and a higher molecular weight was obtained in a lower concentration of alcohols.
27999373	4	61	theme	GMG	616:618	arg1	recovery					596:603	The galactomannan recovery	578:603	The galactomannan recovery of GSG and GMG in 33.3% ethanol	578:635	The galactomannan recovery of GSG and GMG in 33.3% ethanol was 81.7% and 82.5%, respectively, while that in 28.8% isopropanol was 81.3% and 82.9%, respectively.
27999373	4	61	theme	GMG	616:618	arg1	%					645:645	81.7%	641:645	81.7%	641:645	The galactomannan recovery of GSG and GMG in 33.3% ethanol was 81.7% and 82.5%, respectively, while that in 28.8% isopropanol was 81.3% and 82.9%, respectively.
25662512	0	0	theme	c-di-GMP-dependent	88:105	arg1	regulation					107:116	c-di-GMP-dependent regulation	88:116	c-di-GMP-dependent regulation	88:116	Listeria monocytogenes exopolysaccharide: origin, structure, biosynthetic machinery and c-di-GMP-dependent regulation.
25662512	7	1	theme	EPS	1017:1019	arg1	chain					1073:1077	a β-1,4-linked N-acetylmannosamine chain	1038:1077	a β-1,4-linked N-acetylmannosamine chain decorated with terminal α-1,6-linked galactose	1038:1124	Based on carbohydrate composition, linkage and NMR analysis, the structure of the purified EPS is identified as a β-1,4-linked N-acetylmannosamine chain decorated with terminal α-1,6-linked galactose.
25662512	7	1	theme	EPS	1017:1019	arg1	structure					991:999	the structure	987:999	the structure of the purified EPS	987:1019	Based on carbohydrate composition, linkage and NMR analysis, the structure of the purified EPS is identified as a β-1,4-linked N-acetylmannosamine chain decorated with terminal α-1,6-linked galactose.
25662512	8	2	dep	and	1190:1192	arg1	so					1194:1195	so	1194:1195	so	1194:1195	All genes of the pssA-E operon are required for EPS production and so is a separately located pssZ gene.
25662512	10	3	theme	EPS-mediated	1330:1341	arg1	aggregation					1348:1358	EPS-mediated cell aggregation	1330:1358	EPS-mediated cell aggregation	1330:1358	Exogenously added PssZ prevents EPS-mediated cell aggregation and disperses preformed aggregates, whereas an E72Q mutant in the presumed catalytic residue is much less active.
25662512	7	4	link	β-1,4-linked	1040:1051	arg1	chain					1073:1077	a β-1,4-linked N-acetylmannosamine chain	1038:1077	a β-1,4-linked N-acetylmannosamine chain decorated with terminal α-1,6-linked galactose	1038:1124	Based on carbohydrate composition, linkage and NMR analysis, the structure of the purified EPS is identified as a β-1,4-linked N-acetylmannosamine chain decorated with terminal α-1,6-linked galactose.
25662512	7	4	link	β-1,4-linked	1040:1051	arg1	structure					991:999	the structure	987:999	the structure of the purified EPS	987:1019	Based on carbohydrate composition, linkage and NMR analysis, the structure of the purified EPS is identified as a β-1,4-linked N-acetylmannosamine chain decorated with terminal α-1,6-linked galactose.
25662512	2	5	theme	listerial	393:401	arg1	persistence					403:413	listerial persistence	393:413	listerial persistence in the environment	393:432	This EPS strongly protects cells against disinfectants and desiccation, indicating its potential significance for listerial persistence in the environment and for food safety.
25662512	10	6	theme	E72Q	1407:1410	arg1	mutant					1412:1417	an E72Q mutant	1404:1417	an E72Q mutant in the presumed catalytic residue	1404:1451	Exogenously added PssZ prevents EPS-mediated cell aggregation and disperses preformed aggregates, whereas an E72Q mutant in the presumed catalytic residue is much less active.
25662512	10	6	theme	E72Q	1407:1410	arg1	active					1466:1471	active	1466:1471	active	1466:1471	Exogenously added PssZ prevents EPS-mediated cell aggregation and disperses preformed aggregates, whereas an E72Q mutant in the presumed catalytic residue is much less active.
25662512	2	7	theme	potential	366:374	arg1	significance					376:387	its potential significance	362:387	its potential significance for listerial persistence in the environment and for food safety	362:452	This EPS strongly protects cells against disinfectants and desiccation, indicating its potential significance for listerial persistence in the environment and for food safety.
25662512	7	8	link	α-1,6-linked	1103:1114	arg1	galactose					1116:1124	terminal α-1,6-linked galactose	1094:1124	terminal α-1,6-linked galactose	1094:1124	Based on carbohydrate composition, linkage and NMR analysis, the structure of the purified EPS is identified as a β-1,4-linked N-acetylmannosamine chain decorated with terminal α-1,6-linked galactose.
25662512	8	9	theme	EPS	1175:1177	arg1	production					1179:1188	EPS production	1175:1188	EPS production	1175:1188	All genes of the pssA-E operon are required for EPS production and so is a separately located pssZ gene.
25662512	7	10	theme	α-1,6-linked	1103:1114	arg1	galactose					1116:1124	terminal α-1,6-linked galactose	1094:1124	terminal α-1,6-linked galactose	1094:1124	Based on carbohydrate composition, linkage and NMR analysis, the structure of the purified EPS is identified as a β-1,4-linked N-acetylmannosamine chain decorated with terminal α-1,6-linked galactose.
25662512	10	11	theme	cell	1343:1346	arg1	aggregation					1348:1358	EPS-mediated cell aggregation	1330:1358	EPS-mediated cell aggregation	1330:1358	Exogenously added PssZ prevents EPS-mediated cell aggregation and disperses preformed aggregates, whereas an E72Q mutant in the presumed catalytic residue is much less active.
25662512	5	12	theme	cell	843:846	arg1	surface-bound					848:860	cell surface-bound	843:860	cell surface-bound	843:860	Scanning electron microscopy revealed that L. monocytogenes EPS is cell surface-bound.
25662512	7	13	theme	terminal	1094:1101	arg1	galactose					1116:1124	terminal α-1,6-linked galactose	1094:1124	terminal α-1,6-linked galactose	1094:1124	Based on carbohydrate composition, linkage and NMR analysis, the structure of the purified EPS is identified as a β-1,4-linked N-acetylmannosamine chain decorated with terminal α-1,6-linked galactose.
25662512	10	14	theme	catalytic	1435:1443	arg1	residue					1445:1451	the presumed catalytic residue	1422:1451	the presumed catalytic residue	1422:1451	Exogenously added PssZ prevents EPS-mediated cell aggregation and disperses preformed aggregates, whereas an E72Q mutant in the presumed catalytic residue is much less active.
25662512	2	15	theme	food	442:445	arg1	safety					447:452	food safety	442:452	food safety	442:452	This EPS strongly protects cells against disinfectants and desiccation, indicating its potential significance for listerial persistence in the environment and for food safety.
25662512	2	16	from	persistence	403:413	arg1	environment					422:432	the environment	418:432	the environment	418:432	This EPS strongly protects cells against disinfectants and desiccation, indicating its potential significance for listerial persistence in the environment and for food safety.
25662512	11	17	dep	cyclases	1490:1497	arg1	cyclases					1490:1497	The diguanylate cyclases	1474:1497	The diguanylate cyclases DgcA and DgcB	1474:1511	The diguanylate cyclases DgcA and DgcB, whose genes are located next to pssZ, are primarily responsible for c-di-GMP-dependent EPS production.
25662512	11	17	dep	cyclases	1490:1497	arg1	DgcB					1508:1511	DgcB	1508:1511	DgcB	1508:1511	The diguanylate cyclases DgcA and DgcB, whose genes are located next to pssZ, are primarily responsible for c-di-GMP-dependent EPS production.
25662512	11	17	dep	cyclases	1490:1497	arg1	DgcA					1499:1502	DgcA	1499:1502	DgcA	1499:1502	The diguanylate cyclases DgcA and DgcB, whose genes are located next to pssZ, are primarily responsible for c-di-GMP-dependent EPS production.
25662512	10	18	theme	presumed	1426:1433	arg1	residue					1445:1451	the presumed catalytic residue	1422:1451	the presumed catalytic residue	1422:1451	Exogenously added PssZ prevents EPS-mediated cell aggregation and disperses preformed aggregates, whereas an E72Q mutant in the presumed catalytic residue is much less active.
25662512	1	19	theme	unknown	196:202	arg1	exopolysaccharide					204:220	an unknown exopolysaccharide	193:220	an unknown exopolysaccharide (EPS)	193:226	Elevated levels of the second messenger c-di-GMP activate biosynthesis of an unknown exopolysaccharide (EPS) in the food-borne pathogen Listeria monocytogenes.
25662512	1	19	theme	unknown	196:202	arg1	EPS					223:225	EPS	223:225	EPS	223:225	Elevated levels of the second messenger c-di-GMP activate biosynthesis of an unknown exopolysaccharide (EPS) in the food-borne pathogen Listeria monocytogenes.
25662512	11	20	theme	diguanylate	1478:1488	arg1	responsible					1566:1576	responsible	1566:1576	responsible	1566:1576	The diguanylate cyclases DgcA and DgcB, whose genes are located next to pssZ, are primarily responsible for c-di-GMP-dependent EPS production.
25662512	11	20	theme	diguanylate	1478:1488	arg1	cyclases					1490:1497	The diguanylate cyclases	1474:1497	The diguanylate cyclases DgcA and DgcB	1474:1511	The diguanylate cyclases DgcA and DgcB, whose genes are located next to pssZ, are primarily responsible for c-di-GMP-dependent EPS production.
25662512	11	20	theme	diguanylate	1478:1488	arg1	DgcB					1508:1511	DgcB	1508:1511	DgcB	1508:1511	The diguanylate cyclases DgcA and DgcB, whose genes are located next to pssZ, are primarily responsible for c-di-GMP-dependent EPS production.
25662512	11	20	theme	diguanylate	1478:1488	arg1	DgcA					1499:1502	DgcA	1499:1502	DgcA	1499:1502	The diguanylate cyclases DgcA and DgcB, whose genes are located next to pssZ, are primarily responsible for c-di-GMP-dependent EPS production.
25662512	1	21	theme	exopolysaccharide	204:220	arg1	biosynthesis					177:188	biosynthesis	177:188	biosynthesis of an unknown exopolysaccharide (EPS)	177:226	Elevated levels of the second messenger c-di-GMP activate biosynthesis of an unknown exopolysaccharide (EPS) in the food-borne pathogen Listeria monocytogenes.
25662512	0	22	theme	Listeria	0:7	arg1	monocytogenes					9:21	Listeria monocytogenes	0:21	Listeria monocytogenes	0:21	Listeria monocytogenes exopolysaccharide: origin, structure, biosynthetic machinery and c-di-GMP-dependent regulation.
25662512	7	23	theme	carbohydrate	935:946	arg1	composition					948:958	carbohydrate composition	935:958	carbohydrate composition	935:958	Based on carbohydrate composition, linkage and NMR analysis, the structure of the purified EPS is identified as a β-1,4-linked N-acetylmannosamine chain decorated with terminal α-1,6-linked galactose.
25662512	6	24	theme	cell-wall	908:916	arg1	debris					918:923	cell-wall debris	908:923	cell-wall debris	908:923	Secreted carbohydrates represent exclusively cell-wall debris.
25662512	3	25	theme	diguanylate	628:638	arg1	cyclases					640:647	diguanylate cyclases	628:647	diguanylate cyclases activating its synthesis	628:672	We analyzed the potential phylogenetic origin of this EPS, determined its complete structure, characterized genes involved in its biosynthesis and hydrolysis and identified diguanylate cyclases activating its synthesis.
25662512	3	26	theme	potential	471:479	arg1	origin					494:499	the potential phylogenetic origin	467:499	the potential phylogenetic origin of this EPS	467:511	We analyzed the potential phylogenetic origin of this EPS, determined its complete structure, characterized genes involved in its biosynthesis and hydrolysis and identified diguanylate cyclases activating its synthesis.
25662512	7	27	theme	β-1,4-linked	1040:1051	arg1	chain					1073:1077	a β-1,4-linked N-acetylmannosamine chain	1038:1077	a β-1,4-linked N-acetylmannosamine chain decorated with terminal α-1,6-linked galactose	1038:1124	Based on carbohydrate composition, linkage and NMR analysis, the structure of the purified EPS is identified as a β-1,4-linked N-acetylmannosamine chain decorated with terminal α-1,6-linked galactose.
25662512	7	27	theme	β-1,4-linked	1040:1051	arg1	structure					991:999	the structure	987:999	the structure of the purified EPS	987:1019	Based on carbohydrate composition, linkage and NMR analysis, the structure of the purified EPS is identified as a β-1,4-linked N-acetylmannosamine chain decorated with terminal α-1,6-linked galactose.
25662512	5	28	theme	electron	785:792	arg1	microscopy					794:803	Scanning electron microscopy	776:803	Scanning electron microscopy	776:803	Scanning electron microscopy revealed that L. monocytogenes EPS is cell surface-bound.
25662512	7	29	theme	N-acetylmannosamine	1053:1071	arg1	chain					1073:1077	a β-1,4-linked N-acetylmannosamine chain	1038:1077	a β-1,4-linked N-acetylmannosamine chain decorated with terminal α-1,6-linked galactose	1038:1124	Based on carbohydrate composition, linkage and NMR analysis, the structure of the purified EPS is identified as a β-1,4-linked N-acetylmannosamine chain decorated with terminal α-1,6-linked galactose.
25662512	7	29	theme	N-acetylmannosamine	1053:1071	arg1	structure					991:999	the structure	987:999	the structure of the purified EPS	987:1019	Based on carbohydrate composition, linkage and NMR analysis, the structure of the purified EPS is identified as a β-1,4-linked N-acetylmannosamine chain decorated with terminal α-1,6-linked galactose.
25662512	1	30	theme	Elevated	119:126	arg1	levels					128:133	Elevated levels	119:133	Elevated levels of the second messenger c-di-GMP	119:166	Elevated levels of the second messenger c-di-GMP activate biosynthesis of an unknown exopolysaccharide (EPS) in the food-borne pathogen Listeria monocytogenes.
25662512	7	31	theme	NMR	973:975	arg1	analysis					977:984	NMR analysis	973:984	NMR analysis	973:984	Based on carbohydrate composition, linkage and NMR analysis, the structure of the purified EPS is identified as a β-1,4-linked N-acetylmannosamine chain decorated with terminal α-1,6-linked galactose.
25662512	3	32	theme	EPS	509:511	arg1	origin					494:499	the potential phylogenetic origin	467:499	the potential phylogenetic origin of this EPS	467:511	We analyzed the potential phylogenetic origin of this EPS, determined its complete structure, characterized genes involved in its biosynthesis and hydrolysis and identified diguanylate cyclases activating its synthesis.
25662512	9	33	contain	has	1250:1252	arg1	PssZ					1245:1248	PssZ	1245:1248	PssZ	1245:1248	We show that PssZ has an EPS-specific glycosylhydrolase activity.
25662512	9	33	contain	has	1250:1252	arg2	activity					1288:1295	an EPS-specific glycosylhydrolase activity	1254:1295	an EPS-specific glycosylhydrolase activity	1254:1295	We show that PssZ has an EPS-specific glycosylhydrolase activity.
25662512	10	34	from	mutant	1412:1417	arg1	residue					1445:1451	the presumed catalytic residue	1422:1451	the presumed catalytic residue	1422:1451	Exogenously added PssZ prevents EPS-mediated cell aggregation and disperses preformed aggregates, whereas an E72Q mutant in the presumed catalytic residue is much less active.
25662512	11	35	theme	c-di-GMP-dependent	1582:1599	arg1	production					1605:1614	c-di-GMP-dependent EPS production	1582:1614	c-di-GMP-dependent EPS production	1582:1614	The diguanylate cyclases DgcA and DgcB, whose genes are located next to pssZ, are primarily responsible for c-di-GMP-dependent EPS production.
25662512	4	36	theme	EPS	700:702	arg1	proteins					717:724	EPS biosynthesis proteins	700:724	EPS biosynthesis proteins	700:724	Phylogenetic analysis of EPS biosynthesis proteins suggests that they have evolved within monoderms.
25662512	11	37	theme	EPS	1601:1603	arg1	production					1605:1614	c-di-GMP-dependent EPS production	1582:1614	c-di-GMP-dependent EPS production	1582:1614	The diguanylate cyclases DgcA and DgcB, whose genes are located next to pssZ, are primarily responsible for c-di-GMP-dependent EPS production.
25662512	3	38	theme	phylogenetic	481:492	arg1	origin					494:499	the potential phylogenetic origin	467:499	the potential phylogenetic origin of this EPS	467:511	We analyzed the potential phylogenetic origin of this EPS, determined its complete structure, characterized genes involved in its biosynthesis and hydrolysis and identified diguanylate cyclases activating its synthesis.
25662512	8	39	theme	pssZ	1221:1224	arg1	gene					1226:1229	a separately located pssZ gene	1200:1229	a separately located pssZ gene	1200:1229	All genes of the pssA-E operon are required for EPS production and so is a separately located pssZ gene.
25662512	4	40	theme	proteins	717:724	arg1	analysis					688:695	Phylogenetic analysis	675:695	Phylogenetic analysis of EPS biosynthesis proteins	675:724	Phylogenetic analysis of EPS biosynthesis proteins suggests that they have evolved within monoderms.
25662512	3	41	theme	complete	529:536	arg1	structure					538:546	its complete structure	525:546	its complete structure	525:546	We analyzed the potential phylogenetic origin of this EPS, determined its complete structure, characterized genes involved in its biosynthesis and hydrolysis and identified diguanylate cyclases activating its synthesis.
25662512	8	42	theme	located	1213:1219	arg1	gene					1226:1229	a separately located pssZ gene	1200:1229	a separately located pssZ gene	1200:1229	All genes of the pssA-E operon are required for EPS production and so is a separately located pssZ gene.
25662512	6	43	theme	Secreted	863:870	arg1	carbohydrates					872:884	Secreted carbohydrates	863:884	Secreted carbohydrates	863:884	Secreted carbohydrates represent exclusively cell-wall debris.
25662512	8	44	theme	operon	1151:1156	arg1	genes					1131:1135	All genes	1127:1135	All genes of the pssA-E operon	1127:1156	All genes of the pssA-E operon are required for EPS production and so is a separately located pssZ gene.
25662512	8	45	theme	pssA-E	1144:1149	arg1	operon					1151:1156	the pssA-E operon	1140:1156	the pssA-E operon	1140:1156	All genes of the pssA-E operon are required for EPS production and so is a separately located pssZ gene.
25662512	9	46	theme	EPS-specific	1257:1268	arg1	activity					1288:1295	an EPS-specific glycosylhydrolase activity	1254:1295	an EPS-specific glycosylhydrolase activity	1254:1295	We show that PssZ has an EPS-specific glycosylhydrolase activity.
25662512	1	47	theme	second	142:147	arg1	c-di-GMP					159:166	the second messenger c-di-GMP	138:166	the second messenger c-di-GMP	138:166	Elevated levels of the second messenger c-di-GMP activate biosynthesis of an unknown exopolysaccharide (EPS) in the food-borne pathogen Listeria monocytogenes.
25662512	1	48	theme	food-borne	235:244	arg1	monocytogenes					264:276	the food-borne pathogen Listeria monocytogenes	231:276	the food-borne pathogen Listeria monocytogenes	231:276	Elevated levels of the second messenger c-di-GMP activate biosynthesis of an unknown exopolysaccharide (EPS) in the food-borne pathogen Listeria monocytogenes.
25662512	5	49	theme	Scanning	776:783	arg1	microscopy					794:803	Scanning electron microscopy	776:803	Scanning electron microscopy	776:803	Scanning electron microscopy revealed that L. monocytogenes EPS is cell surface-bound.
25662512	0	50	dep	exopolysaccharide	23:39	arg1	monocytogenes					9:21	Listeria monocytogenes	0:21	Listeria monocytogenes	0:21	Listeria monocytogenes exopolysaccharide: origin, structure, biosynthetic machinery and c-di-GMP-dependent regulation.
25662512	9	51	theme	glycosylhydrolase	1270:1286	arg1	activity					1288:1295	an EPS-specific glycosylhydrolase activity	1254:1295	an EPS-specific glycosylhydrolase activity	1254:1295	We show that PssZ has an EPS-specific glycosylhydrolase activity.
25662512	1	52	theme	messenger	149:157	arg1	c-di-GMP					159:166	the second messenger c-di-GMP	138:166	the second messenger c-di-GMP	138:166	Elevated levels of the second messenger c-di-GMP activate biosynthesis of an unknown exopolysaccharide (EPS) in the food-borne pathogen Listeria monocytogenes.
25662512	1	53	theme	pathogen	246:253	arg1	monocytogenes					264:276	the food-borne pathogen Listeria monocytogenes	231:276	the food-borne pathogen Listeria monocytogenes	231:276	Elevated levels of the second messenger c-di-GMP activate biosynthesis of an unknown exopolysaccharide (EPS) in the food-borne pathogen Listeria monocytogenes.
25662512	10	54	dep	prevents	1321:1328	arg1	whereas					1396:1402	whereas	1396:1402	whereas	1396:1402	Exogenously added PssZ prevents EPS-mediated cell aggregation and disperses preformed aggregates, whereas an E72Q mutant in the presumed catalytic residue is much less active.
25662512	4	55	theme	biosynthesis	704:715	arg1	proteins					717:724	EPS biosynthesis proteins	700:724	EPS biosynthesis proteins	700:724	Phylogenetic analysis of EPS biosynthesis proteins suggests that they have evolved within monoderms.
25662512	1	56	theme	c-di-GMP	159:166	arg1	levels					128:133	Elevated levels	119:133	Elevated levels of the second messenger c-di-GMP	119:166	Elevated levels of the second messenger c-di-GMP activate biosynthesis of an unknown exopolysaccharide (EPS) in the food-borne pathogen Listeria monocytogenes.
25662512	10	57	theme	preformed	1374:1382	arg1	aggregates					1384:1393	preformed aggregates	1374:1393	preformed aggregates	1374:1393	Exogenously added PssZ prevents EPS-mediated cell aggregation and disperses preformed aggregates, whereas an E72Q mutant in the presumed catalytic residue is much less active.
25662512	1	58	theme	Listeria	255:262	arg1	monocytogenes					264:276	the food-borne pathogen Listeria monocytogenes	231:276	the food-borne pathogen Listeria monocytogenes	231:276	Elevated levels of the second messenger c-di-GMP activate biosynthesis of an unknown exopolysaccharide (EPS) in the food-borne pathogen Listeria monocytogenes.
25662512	0	59	theme	biosynthetic	61:72	arg1	machinery					74:82	biosynthetic machinery	61:82	biosynthetic machinery	61:82	Listeria monocytogenes exopolysaccharide: origin, structure, biosynthetic machinery and c-di-GMP-dependent regulation.
25662512	4	60	theme	Phylogenetic	675:686	arg1	analysis					688:695	Phylogenetic analysis	675:695	Phylogenetic analysis of EPS biosynthesis proteins	675:724	Phylogenetic analysis of EPS biosynthesis proteins suggests that they have evolved within monoderms.
25662512	7	61	theme	purified	1008:1015	arg1	EPS					1017:1019	the purified EPS	1004:1019	the purified EPS	1004:1019	Based on carbohydrate composition, linkage and NMR analysis, the structure of the purified EPS is identified as a β-1,4-linked N-acetylmannosamine chain decorated with terminal α-1,6-linked galactose.
25662512	10	62	theme	added	1310:1314	arg1	PssZ					1316:1319	Exogenously added PssZ	1298:1319	Exogenously added PssZ	1298:1319	Exogenously added PssZ prevents EPS-mediated cell aggregation and disperses preformed aggregates, whereas an E72Q mutant in the presumed catalytic residue is much less active.
28578953	0	0	theme	adsorption	73:82	arg1	properties					84:93	its dielectric and lead adsorption properties	49:93	properties	84:93	Calcinated tea and cellulose composite films and its dielectric and lead adsorption properties.
28578953	5	1	theme	adsorption	753:762	arg1	performance					764:774	its adsorption performance	749:774	its adsorption performance	749:774	In addition, the CTCC films adsorb Pb2+ ions and its adsorption performance depends on the calcinated tea content and pH level.
28578953	3	2	from	effect	453:458	arg1	properties					493:502	the properties	489:502	the properties of the prepared CTCC films	489:529	The effect of calcinated tea loading on the properties of the prepared CTCC films was studied.
28578953	1	3	theme	composite	140:148	arg1	films					157:161	calcinated tea and cellulose composite (CTCC) films	111:161	calcinated tea and cellulose composite (CTCC) films	111:161	In this paper, calcinated tea and cellulose composite (CTCC) films were fabricated via solution casting method.
28578953	4	4	theme	CTCC	582:585	arg1	films					587:591	the prepared CTCC films	569:591	the prepared CTCC films	569:591	The results suggest that the prepared CTCC films show higher mechanical properties, thermal stability and dielectric constant than the neat cellulose film.
28578953	0	5	theme	lead	68:71	arg1	properties					84:93	its dielectric and lead adsorption properties	49:93	properties	84:93	Calcinated tea and cellulose composite films and its dielectric and lead adsorption properties.
28578953	3	6	theme	prepared	511:518	arg1	films					525:529	the prepared CTCC films	507:529	the prepared CTCC films	507:529	The effect of calcinated tea loading on the properties of the prepared CTCC films was studied.
28578953	4	7	theme	prepared	573:580	arg1	films					587:591	the prepared CTCC films	569:591	the prepared CTCC films	569:591	The results suggest that the prepared CTCC films show higher mechanical properties, thermal stability and dielectric constant than the neat cellulose film.
28578953	3	8	theme	calcinated	463:472	arg1	loading					478:484	calcinated tea loading	463:484	calcinated tea loading	463:484	The effect of calcinated tea loading on the properties of the prepared CTCC films was studied.
28578953	2	9	dep	transform	336:344	arg1	infrared					346:353	infrared	346:353	transform infrared spectroscopy, scanning electron microscopy, X-ray diffraction and thermogravimetric analysis	336:446	Chemical structure, morphology, crystallinity and thermal stability of the fabricated films were characterized by using Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray diffraction and thermogravimetric analysis.
28578953	5	10	theme	Pb2+	735:738	arg1	ions					740:743	Pb2+ ions	735:743	Pb2+ ions	735:743	In addition, the CTCC films adsorb Pb2+ ions and its adsorption performance depends on the calcinated tea content and pH level.
28578953	6	11	theme	flexible	867:874	arg1	capacitor					876:884	flexible capacitor	867:884	flexible capacitor	867:884	The CTCC films are useful for sensors, flexible capacitor as well as lead adsorption applications.
28578953	4	12	theme	thermal	628:634	arg1	stability					636:644	thermal stability	628:644	thermal stability	628:644	The results suggest that the prepared CTCC films show higher mechanical properties, thermal stability and dielectric constant than the neat cellulose film.
28578953	2	13	theme	thermal	258:264	arg1	stability					266:274	thermal stability	258:274	thermal stability	258:274	Chemical structure, morphology, crystallinity and thermal stability of the fabricated films were characterized by using Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray diffraction and thermogravimetric analysis.
28578953	4	14	theme	neat	679:682	arg1	film					694:697	the neat cellulose film	675:697	the neat cellulose film	675:697	The results suggest that the prepared CTCC films show higher mechanical properties, thermal stability and dielectric constant than the neat cellulose film.
28578953	5	15	theme	tea	802:804	arg1	content					806:812	the calcinated tea content	787:812	the calcinated tea content	787:812	In addition, the CTCC films adsorb Pb2+ ions and its adsorption performance depends on the calcinated tea content and pH level.
28578953	0	16	theme	tea	11:13	arg1	films					39:43	Calcinated tea and cellulose composite films	0:43	Calcinated tea and cellulose composite films	0:43	Calcinated tea and cellulose composite films and its dielectric and lead adsorption properties.
28578953	3	17	theme	films	525:529	arg1	properties					493:502	the properties	489:502	the properties of the prepared CTCC films	489:529	The effect of calcinated tea loading on the properties of the prepared CTCC films was studied.
28578953	2	18	theme	electron	378:385	arg1	microscopy					387:396	scanning electron microscopy	369:396	scanning electron microscopy	369:396	Chemical structure, morphology, crystallinity and thermal stability of the fabricated films were characterized by using Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray diffraction and thermogravimetric analysis.
28578953	0	19	theme	Calcinated	0:9	arg1	films					39:43	Calcinated tea and cellulose composite films	0:43	Calcinated tea and cellulose composite films	0:43	Calcinated tea and cellulose composite films and its dielectric and lead adsorption properties.
28578953	2	20	theme	films	294:298	arg1	stability					266:274	thermal stability	258:274	thermal stability	258:274	Chemical structure, morphology, crystallinity and thermal stability of the fabricated films were characterized by using Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray diffraction and thermogravimetric analysis.
28578953	2	20	theme	films	294:298	arg1	morphology					228:237	morphology	228:237	morphology	228:237	Chemical structure, morphology, crystallinity and thermal stability of the fabricated films were characterized by using Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray diffraction and thermogravimetric analysis.
28578953	2	20	theme	films	294:298	arg1	structure					217:225	Chemical structure	208:225	Chemical structure	208:225	Chemical structure, morphology, crystallinity and thermal stability of the fabricated films were characterized by using Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray diffraction and thermogravimetric analysis.
28578953	2	20	theme	films	294:298	arg1	crystallinity					240:252	crystallinity	240:252	crystallinity	240:252	Chemical structure, morphology, crystallinity and thermal stability of the fabricated films were characterized by using Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray diffraction and thermogravimetric analysis.
28578953	2	21	theme	scanning	369:376	arg1	microscopy					387:396	scanning electron microscopy	369:396	scanning electron microscopy	369:396	Chemical structure, morphology, crystallinity and thermal stability of the fabricated films were characterized by using Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray diffraction and thermogravimetric analysis.
28578953	0	22	theme	cellulose	19:27	arg1	films					39:43	Calcinated tea and cellulose composite films	0:43	Calcinated tea and cellulose composite films	0:43	Calcinated tea and cellulose composite films and its dielectric and lead adsorption properties.
28578953	2	23	dep	Fourier	328:334	arg1	transform					336:344	transform	336:344	transform infrared spectroscopy, scanning electron microscopy, X-ray diffraction and thermogravimetric analysis	336:446	Chemical structure, morphology, crystallinity and thermal stability of the fabricated films were characterized by using Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray diffraction and thermogravimetric analysis.
28578953	2	24	theme	thermogravimetric	421:437	arg1	analysis					439:446	thermogravimetric analysis	421:446	thermogravimetric analysis	421:446	Chemical structure, morphology, crystallinity and thermal stability of the fabricated films were characterized by using Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray diffraction and thermogravimetric analysis.
28578953	5	25	theme	pH	818:819	arg1	level					821:825	pH level	818:825	pH level	818:825	In addition, the CTCC films adsorb Pb2+ ions and its adsorption performance depends on the calcinated tea content and pH level.
28578953	3	26	theme	loading	478:484	arg1	effect					453:458	The effect	449:458	The effect of calcinated tea loading on the properties of the prepared CTCC films	449:529	The effect of calcinated tea loading on the properties of the prepared CTCC films was studied.
28578953	4	27	theme	dielectric	650:659	arg1	constant					661:668	dielectric constant	650:668	dielectric constant	650:668	The results suggest that the prepared CTCC films show higher mechanical properties, thermal stability and dielectric constant than the neat cellulose film.
28578953	1	28	theme	solution	183:190	arg1	method					200:205	solution casting method	183:205	solution casting method	183:205	In this paper, calcinated tea and cellulose composite (CTCC) films were fabricated via solution casting method.
28578953	0	29	theme	composite	29:37	arg1	films					39:43	Calcinated tea and cellulose composite films	0:43	Calcinated tea and cellulose composite films	0:43	Calcinated tea and cellulose composite films and its dielectric and lead adsorption properties.
28578953	5	30	theme	CTCC	717:720	arg1	films					722:726	the CTCC films	713:726	the CTCC films	713:726	In addition, the CTCC films adsorb Pb2+ ions and its adsorption performance depends on the calcinated tea content and pH level.
28578953	2	31	theme	Chemical	208:215	arg1	structure					217:225	Chemical structure	208:225	Chemical structure	208:225	Chemical structure, morphology, crystallinity and thermal stability of the fabricated films were characterized by using Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray diffraction and thermogravimetric analysis.
28578953	2	32	theme	X-ray	399:403	arg1	diffraction					405:415	X-ray diffraction	399:415	X-ray diffraction	399:415	Chemical structure, morphology, crystallinity and thermal stability of the fabricated films were characterized by using Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray diffraction and thermogravimetric analysis.
28578953	5	33	theme	calcinated	791:800	arg1	content					806:812	the calcinated tea content	787:812	the calcinated tea content	787:812	In addition, the CTCC films adsorb Pb2+ ions and its adsorption performance depends on the calcinated tea content and pH level.
28578953	6	34	theme	CTCC	832:835	arg1	useful					847:852	useful	847:852	useful	847:852	The CTCC films are useful for sensors, flexible capacitor as well as lead adsorption applications.
28578953	6	34	theme	CTCC	832:835	arg1	films					837:841	The CTCC films	828:841	The CTCC films	828:841	The CTCC films are useful for sensors, flexible capacitor as well as lead adsorption applications.
28578953	6	35	theme	adsorption	902:911	arg1	applications					913:924	lead adsorption applications	897:924	lead adsorption applications	897:924	The CTCC films are useful for sensors, flexible capacitor as well as lead adsorption applications.
28578953	4	36	theme	cellulose	684:692	arg1	film					694:697	the neat cellulose film	675:697	the neat cellulose film	675:697	The results suggest that the prepared CTCC films show higher mechanical properties, thermal stability and dielectric constant than the neat cellulose film.
28578953	1	37	theme	calcinated	111:120	arg1	tea					122:124	calcinated tea	111:124	calcinated tea	111:124	In this paper, calcinated tea and cellulose composite (CTCC) films were fabricated via solution casting method.
28578953	2	38	theme	fabricated	283:292	arg1	films					294:298	the fabricated films	279:298	the fabricated films	279:298	Chemical structure, morphology, crystallinity and thermal stability of the fabricated films were characterized by using Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray diffraction and thermogravimetric analysis.
28578953	4	39	theme	mechanical	605:614	arg1	properties					616:625	higher mechanical properties	598:625	higher mechanical properties	598:625	The results suggest that the prepared CTCC films show higher mechanical properties, thermal stability and dielectric constant than the neat cellulose film.
28578953	1	40	theme	tea	122:124	arg1	films					157:161	calcinated tea and cellulose composite (CTCC) films	111:161	calcinated tea and cellulose composite (CTCC) films	111:161	In this paper, calcinated tea and cellulose composite (CTCC) films were fabricated via solution casting method.
28578953	4	41	theme	higher	598:603	arg1	properties					616:625	higher mechanical properties	598:625	higher mechanical properties	598:625	The results suggest that the prepared CTCC films show higher mechanical properties, thermal stability and dielectric constant than the neat cellulose film.
28578953	1	42	theme	casting	192:198	arg1	method					200:205	solution casting method	183:205	solution casting method	183:205	In this paper, calcinated tea and cellulose composite (CTCC) films were fabricated via solution casting method.
28578953	3	43	theme	tea	474:476	arg1	loading					478:484	calcinated tea loading	463:484	calcinated tea loading	463:484	The effect of calcinated tea loading on the properties of the prepared CTCC films was studied.
28578953	3	44	theme	CTCC	520:523	arg1	films					525:529	the prepared CTCC films	507:529	the prepared CTCC films	507:529	The effect of calcinated tea loading on the properties of the prepared CTCC films was studied.
28578953	6	45	theme	lead	897:900	arg1	applications					913:924	lead adsorption applications	897:924	lead adsorption applications	897:924	The CTCC films are useful for sensors, flexible capacitor as well as lead adsorption applications.
28578953	1	46	theme	cellulose	130:138	arg1	CTCC					151:154	CTCC	151:154	CTCC	151:154	In this paper, calcinated tea and cellulose composite (CTCC) films were fabricated via solution casting method.
28578953	1	46	theme	cellulose	130:138	arg1	composite					140:148	cellulose composite	130:148	cellulose composite (CTCC)	130:155	In this paper, calcinated tea and cellulose composite (CTCC) films were fabricated via solution casting method.
25966324	7	0	theme	composition	1321:1331	arg1	components					1333:1342	all composition components	1317:1342	all composition components assessed for both sets of hybrids	1317:1376	Overall, results highlighted extensive variation in all composition components assessed for both sets of hybrids.
25966324	4	1	theme	tocopherols	946:956	arg1	measurements					842:853	measurements	842:853	measurements of proximates (protein, fat, ash, and starch), fibers, minerals, amino acids, fatty acids, tocopherols (α-, γ-, and δ-), β-carotene, phytic acid, and raffinose	842:1013	The compositional analysis of B73 hybrids from the 25 NAM founders and 24 inbred lines derived from landraces included measurements of proximates (protein, fat, ash, and starch), fibers, minerals, amino acids, fatty acids, tocopherols (α-, γ-, and δ-), β-carotene, phytic acid, and raffinose.
25966324	7	2	theme	extensive	1294:1302	arg1	variation					1304:1312	extensive variation	1294:1312	extensive variation	1294:1312	Overall, results highlighted extensive variation in all composition components assessed for both sets of hybrids.
25966324	9	3	from	community	1724:1732	arg1	development					1741:1751	the development	1737:1751	the development of new improved lines	1737:1773	The study described here provided significant information on contributions of conventional breeding to crop compositional variation, as well as valuable information on key genetic resources for the maize community in the development of new improved lines.
25966324	4	4	theme	phytic	988:993	arg1	acid					995:998	phytic acid	988:998	phytic acid	988:998	The compositional analysis of B73 hybrids from the 25 NAM founders and 24 inbred lines derived from landraces included measurements of proximates (protein, fat, ash, and starch), fibers, minerals, amino acids, fatty acids, tocopherols (α-, γ-, and δ-), β-carotene, phytic acid, and raffinose.
25966324	9	5	theme	lines	1769:1773	arg1	development					1741:1751	the development	1737:1751	the development of new improved lines	1737:1773	The study described here provided significant information on contributions of conventional breeding to crop compositional variation, as well as valuable information on key genetic resources for the maize community in the development of new improved lines.
25966324	3	6	theme	maize	710:714	arg1	lines					716:720	modern maize lines	703:720	modern maize lines	703:720	Landraces are also of interest to the maize community as they offer the potential to discover new alleles that could be incorporated into modern maize lines.
25966324	6	7	theme	distinct	1169:1176	arg1	groups					1187:1192	distinct breeding groups	1169:1192	distinct breeding groups (tropical, temperate, flint, mixed/intermediate)	1169:1241	For each data set (NAM and landrace) canonical discriminant analysis allowed separation of distinct breeding groups (tropical, temperate, flint, mixed/intermediate) within each data set.
25966324	4	8	theme	β-carotene	976:985	arg1	measurements					842:853	measurements	842:853	measurements of proximates (protein, fat, ash, and starch), fibers, minerals, amino acids, fatty acids, tocopherols (α-, γ-, and δ-), β-carotene, phytic acid, and raffinose	842:1013	The compositional analysis of B73 hybrids from the 25 NAM founders and 24 inbred lines derived from landraces included measurements of proximates (protein, fat, ash, and starch), fibers, minerals, amino acids, fatty acids, tocopherols (α-, γ-, and δ-), β-carotene, phytic acid, and raffinose.
25966324	9	9	theme	significant	1554:1564	arg1	information					1566:1576	significant information	1554:1576	significant information on contributions of conventional breeding to crop compositional variation	1554:1650	The study described here provided significant information on contributions of conventional breeding to crop compositional variation, as well as valuable information on key genetic resources for the maize community in the development of new improved lines.
25966324	0	10	theme	landrace	124:131	arg1	lines					140:144	diverse landrace inbred lines	116:144	diverse landrace inbred lines	116:144	Compositional assessments of key maize populations: B73 hybrids of the Nested Association Mapping founder lines and diverse landrace inbred lines.
25966324	4	11	from	founders	781:788	arg1	hybrids					757:763	B73 hybrids	753:763	B73 hybrids from the 25 NAM founders and 24 inbred lines derived from landraces	753:831	The compositional analysis of B73 hybrids from the 25 NAM founders and 24 inbred lines derived from landraces included measurements of proximates (protein, fat, ash, and starch), fibers, minerals, amino acids, fatty acids, tocopherols (α-, γ-, and δ-), β-carotene, phytic acid, and raffinose.
25966324	4	11	from	founders	781:788	arg1	analysis					741:748	The compositional analysis	723:748	The compositional analysis of B73 hybrids from the 25 NAM founders and 24 inbred lines derived from landraces	723:831	The compositional analysis of B73 hybrids from the 25 NAM founders and 24 inbred lines derived from landraces included measurements of proximates (protein, fat, ash, and starch), fibers, minerals, amino acids, fatty acids, tocopherols (α-, γ-, and δ-), β-carotene, phytic acid, and raffinose.
25966324	1	12	theme	diversity	209:217	arg1	assessment					177:186	an assessment	174:186	an assessment of the compositional diversity in maize B73 hybrids derived both from the Nested Association Mapping (NAM) founder lines and from a diverse collection of landrace accessions from North and South America	174:389	The present study provides an assessment of the compositional diversity in maize B73 hybrids derived both from the Nested Association Mapping (NAM) founder lines and from a diverse collection of landrace accessions from North and South America.
25966324	2	13	theme	complex	549:555	arg1	traits					557:562	complex traits	549:562	complex traits	549:562	The NAM founders represent a key population of publicly available lines that are used extensively in the maize community to investigate the genetic basis of complex traits.
25966324	1	14	from	hybrids	232:238	arg1	assessment					177:186	an assessment	174:186	an assessment of the compositional diversity in maize B73 hybrids derived both from the Nested Association Mapping (NAM) founder lines and from a diverse collection of landrace accessions from North and South America	174:389	The present study provides an assessment of the compositional diversity in maize B73 hybrids derived both from the Nested Association Mapping (NAM) founder lines and from a diverse collection of landrace accessions from North and South America.
25966324	4	15	attach	derived	810:816	arg1	landraces					823:831	landraces	823:831	landraces	823:831	The compositional analysis of B73 hybrids from the 25 NAM founders and 24 inbred lines derived from landraces included measurements of proximates (protein, fat, ash, and starch), fibers, minerals, amino acids, fatty acids, tocopherols (α-, γ-, and δ-), β-carotene, phytic acid, and raffinose.
25966324	4	15	attach	derived	810:816	arg2	lines					804:808	24 inbred lines	794:808	24 inbred lines derived from landraces	794:831	The compositional analysis of B73 hybrids from the 25 NAM founders and 24 inbred lines derived from landraces included measurements of proximates (protein, fat, ash, and starch), fibers, minerals, amino acids, fatty acids, tocopherols (α-, γ-, and δ-), β-carotene, phytic acid, and raffinose.
25966324	6	16	theme	canonical	1115:1123	arg1	analysis					1138:1145	canonical discriminant analysis	1115:1145	canonical discriminant analysis	1115:1145	For each data set (NAM and landrace) canonical discriminant analysis allowed separation of distinct breeding groups (tropical, temperate, flint, mixed/intermediate) within each data set.
25966324	1	17	attach	derived	240:246	arg2	hybrids					232:238	maize B73 hybrids	222:238	maize B73 hybrids derived both from the Nested Association Mapping (NAM) founder lines and from a diverse collection of landrace accessions from North and South America	222:389	The present study provides an assessment of the compositional diversity in maize B73 hybrids derived both from the Nested Association Mapping (NAM) founder lines and from a diverse collection of landrace accessions from North and South America.
25966324	1	17	attach	derived	240:246	arg1	collection					328:337	a diverse collection	318:337	a diverse collection of landrace accessions	318:360	The present study provides an assessment of the compositional diversity in maize B73 hybrids derived both from the Nested Association Mapping (NAM) founder lines and from a diverse collection of landrace accessions from North and South America.
25966324	1	17	attach	derived	240:246	arg1	America					383:389	America	383:389	America	383:389	The present study provides an assessment of the compositional diversity in maize B73 hybrids derived both from the Nested Association Mapping (NAM) founder lines and from a diverse collection of landrace accessions from North and South America.
25966324	1	17	attach	derived	240:246	arg1	lines					303:307	the Nested Association Mapping (NAM) founder lines	258:307	the Nested Association Mapping (NAM) founder lines	258:307	The present study provides an assessment of the compositional diversity in maize B73 hybrids derived both from the Nested Association Mapping (NAM) founder lines and from a diverse collection of landrace accessions from North and South America.
25966324	4	18	theme	raffinose	1005:1013	arg1	measurements					842:853	measurements	842:853	measurements of proximates (protein, fat, ash, and starch), fibers, minerals, amino acids, fatty acids, tocopherols (α-, γ-, and δ-), β-carotene, phytic acid, and raffinose	842:1013	The compositional analysis of B73 hybrids from the 25 NAM founders and 24 inbred lines derived from landraces included measurements of proximates (protein, fat, ash, and starch), fibers, minerals, amino acids, fatty acids, tocopherols (α-, γ-, and δ-), β-carotene, phytic acid, and raffinose.
25966324	4	19	dep	proximates	858:867	arg1	protein					870:876	protein	870:876	protein	870:876	The compositional analysis of B73 hybrids from the 25 NAM founders and 24 inbred lines derived from landraces included measurements of proximates (protein, fat, ash, and starch), fibers, minerals, amino acids, fatty acids, tocopherols (α-, γ-, and δ-), β-carotene, phytic acid, and raffinose.
25966324	9	20	theme	valuable	1664:1671	arg1	information					1673:1683	valuable information	1664:1683	valuable information on key genetic resources for the maize community in the development of new improved lines	1664:1773	The study described here provided significant information on contributions of conventional breeding to crop compositional variation, as well as valuable information on key genetic resources for the maize community in the development of new improved lines.
25966324	9	21	from	information	1566:1576	arg1	resources					1700:1708	key genetic resources	1688:1708	key genetic resources for the maize community in the development of new improved lines	1688:1773	The study described here provided significant information on contributions of conventional breeding to crop compositional variation, as well as valuable information on key genetic resources for the maize community in the development of new improved lines.
25966324	9	21	from	information	1566:1576	arg1	contributions					1581:1593	contributions	1581:1593	contributions of conventional breeding to crop compositional variation	1581:1650	The study described here provided significant information on contributions of conventional breeding to crop compositional variation, as well as valuable information on key genetic resources for the maize community in the development of new improved lines.
25966324	9	22	theme	breeding	1611:1618	arg1	contributions					1581:1593	contributions	1581:1593	contributions of conventional breeding to crop compositional variation	1581:1650	The study described here provided significant information on contributions of conventional breeding to crop compositional variation, as well as valuable information on key genetic resources for the maize community in the development of new improved lines.
25966324	2	23	theme	genetic	532:538	arg1	basis					540:544	the genetic basis	528:544	the genetic basis of complex traits	528:562	The NAM founders represent a key population of publicly available lines that are used extensively in the maize community to investigate the genetic basis of complex traits.
25966324	6	24	dep	groups	1187:1192	arg1	mixed/intermediate					1223:1240	mixed/intermediate	1223:1240	mixed/intermediate	1223:1240	For each data set (NAM and landrace) canonical discriminant analysis allowed separation of distinct breeding groups (tropical, temperate, flint, mixed/intermediate) within each data set.
25966324	6	24	dep	groups	1187:1192	arg1	flint					1216:1220	flint	1216:1220	flint	1216:1220	For each data set (NAM and landrace) canonical discriminant analysis allowed separation of distinct breeding groups (tropical, temperate, flint, mixed/intermediate) within each data set.
25966324	4	25	from	analysis	741:748	arg1	lines					804:808	24 inbred lines	794:808	24 inbred lines derived from landraces	794:831	The compositional analysis of B73 hybrids from the 25 NAM founders and 24 inbred lines derived from landraces included measurements of proximates (protein, fat, ash, and starch), fibers, minerals, amino acids, fatty acids, tocopherols (α-, γ-, and δ-), β-carotene, phytic acid, and raffinose.
25966324	4	25	from	analysis	741:748	arg1	founders					781:788	the 25 NAM founders	770:788	the 25 NAM founders	770:788	The compositional analysis of B73 hybrids from the 25 NAM founders and 24 inbred lines derived from landraces included measurements of proximates (protein, fat, ash, and starch), fibers, minerals, amino acids, fatty acids, tocopherols (α-, γ-, and δ-), β-carotene, phytic acid, and raffinose.
25966324	1	26	theme	present	151:157	arg1	study					159:163	The present study	147:163	The present study	147:163	The present study provides an assessment of the compositional diversity in maize B73 hybrids derived both from the Nested Association Mapping (NAM) founder lines and from a diverse collection of landrace accessions from North and South America.
25966324	2	27	theme	maize	497:501	arg1	community					503:511	the maize community	493:511	the maize community	493:511	The NAM founders represent a key population of publicly available lines that are used extensively in the maize community to investigate the genetic basis of complex traits.
25966324	0	28	theme	Association	78:88	arg1	hybrids					56:62	B73 hybrids	52:62	Compositional assessments of key maize populations: B73 hybrids of the Nested Association Mapping founder lines and diverse landrace inbred lines.	0:145	Compositional assessments of key maize populations: B73 hybrids of the Nested Association Mapping founder lines and diverse landrace inbred lines.
25966324	4	29	from	lines	804:808	arg1	hybrids					757:763	B73 hybrids	753:763	B73 hybrids from the 25 NAM founders and 24 inbred lines derived from landraces	753:831	The compositional analysis of B73 hybrids from the 25 NAM founders and 24 inbred lines derived from landraces included measurements of proximates (protein, fat, ash, and starch), fibers, minerals, amino acids, fatty acids, tocopherols (α-, γ-, and δ-), β-carotene, phytic acid, and raffinose.
25966324	4	29	from	lines	804:808	arg1	analysis					741:748	The compositional analysis	723:748	The compositional analysis of B73 hybrids from the 25 NAM founders and 24 inbred lines derived from landraces	723:831	The compositional analysis of B73 hybrids from the 25 NAM founders and 24 inbred lines derived from landraces included measurements of proximates (protein, fat, ash, and starch), fibers, minerals, amino acids, fatty acids, tocopherols (α-, γ-, and δ-), β-carotene, phytic acid, and raffinose.
25966324	6	30	dep	tropical	1195:1202	arg1	temperate					1205:1213	temperate	1205:1213	temperate	1205:1213	For each data set (NAM and landrace) canonical discriminant analysis allowed separation of distinct breeding groups (tropical, temperate, flint, mixed/intermediate) within each data set.
25966324	8	31	from	levels	1490:1495	arg1	lines					1513:1517	modern maize lines	1500:1517	modern maize lines	1500:1517	The variation observed for some components within the landraces may therefore be of value for increasing their levels in modern maize lines.
25966324	0	32	theme	founder	98:104	arg1	lines					106:110	founder lines	98:110	founder lines	98:110	Compositional assessments of key maize populations: B73 hybrids of the Nested Association Mapping founder lines and diverse landrace inbred lines.
25966324	1	33	theme	landrace	342:349	arg1	accessions					351:360	landrace accessions	342:360	landrace accessions	342:360	The present study provides an assessment of the compositional diversity in maize B73 hybrids derived both from the Nested Association Mapping (NAM) founder lines and from a diverse collection of landrace accessions from North and South America.
25966324	2	34	theme	key	421:423	arg1	population					425:434	a key population	419:434	a key population of publicly available lines that are used extensively in the maize community to investigate the genetic basis of complex traits	419:562	The NAM founders represent a key population of publicly available lines that are used extensively in the maize community to investigate the genetic basis of complex traits.
25966324	0	35	theme	Compositional	0:12	arg1	assessments					14:24	Compositional assessments	0:24	Compositional assessments of key maize populations: B73 hybrids of the Nested Association Mapping founder lines and diverse landrace inbred lines.	0:145	Compositional assessments of key maize populations: B73 hybrids of the Nested Association Mapping founder lines and diverse landrace inbred lines.
25966324	1	36	theme	Nested	262:267	arg1	Mapping					281:287	the Nested Association Mapping	258:287	the Nested Association Mapping (NAM) founder lines	258:307	The present study provides an assessment of the compositional diversity in maize B73 hybrids derived both from the Nested Association Mapping (NAM) founder lines and from a diverse collection of landrace accessions from North and South America.
25966324	1	36	theme	Nested	262:267	arg1	NAM					290:292	NAM	290:292	NAM	290:292	The present study provides an assessment of the compositional diversity in maize B73 hybrids derived both from the Nested Association Mapping (NAM) founder lines and from a diverse collection of landrace accessions from North and South America.
25966324	2	37	theme	NAM	396:398	arg1	founders					400:407	The NAM founders	392:407	The NAM founders	392:407	The NAM founders represent a key population of publicly available lines that are used extensively in the maize community to investigate the genetic basis of complex traits.
25966324	6	38	theme	data	1255:1258	arg1	set					1260:1262	each data set	1250:1262	each data set	1250:1262	For each data set (NAM and landrace) canonical discriminant analysis allowed separation of distinct breeding groups (tropical, temperate, flint, mixed/intermediate) within each data set.
25966324	1	39	theme	Mapping	281:287	arg1	lines					303:307	the Nested Association Mapping (NAM) founder lines	258:307	the Nested Association Mapping (NAM) founder lines	258:307	The present study provides an assessment of the compositional diversity in maize B73 hybrids derived both from the Nested Association Mapping (NAM) founder lines and from a diverse collection of landrace accessions from North and South America.
25966324	7	40	theme	hybrids	1370:1376	arg1	hybrids					1370:1376	hybrids	1370:1376	hybrids	1370:1376	Overall, results highlighted extensive variation in all composition components assessed for both sets of hybrids.
25966324	7	40	theme	hybrids	1370:1376	arg1	sets					1362:1365	both sets	1357:1365	both sets of hybrids	1357:1376	Overall, results highlighted extensive variation in all composition components assessed for both sets of hybrids.
25966324	4	41	dep	protein	870:876	arg1	ash					884:886	ash	884:886	ash	884:886	The compositional analysis of B73 hybrids from the 25 NAM founders and 24 inbred lines derived from landraces included measurements of proximates (protein, fat, ash, and starch), fibers, minerals, amino acids, fatty acids, tocopherols (α-, γ-, and δ-), β-carotene, phytic acid, and raffinose.
25966324	4	41	dep	protein	870:876	arg1	starch					893:898	starch	893:898	starch	893:898	The compositional analysis of B73 hybrids from the 25 NAM founders and 24 inbred lines derived from landraces included measurements of proximates (protein, fat, ash, and starch), fibers, minerals, amino acids, fatty acids, tocopherols (α-, γ-, and δ-), β-carotene, phytic acid, and raffinose.
25966324	4	41	dep	protein	870:876	arg1	fat					879:881	fat	879:881	fat	879:881	The compositional analysis of B73 hybrids from the 25 NAM founders and 24 inbred lines derived from landraces included measurements of proximates (protein, fat, ash, and starch), fibers, minerals, amino acids, fatty acids, tocopherols (α-, γ-, and δ-), β-carotene, phytic acid, and raffinose.
25966324	2	42	theme	available	448:456	arg1	lines					458:462	publicly available lines	439:462	publicly available lines that are used extensively in the maize community to investigate the genetic basis of complex traits	439:562	The NAM founders represent a key population of publicly available lines that are used extensively in the maize community to investigate the genetic basis of complex traits.
25966324	9	43	theme	new	1756:1758	arg1	lines					1769:1773	new improved lines	1756:1773	new improved lines	1756:1773	The study described here provided significant information on contributions of conventional breeding to crop compositional variation, as well as valuable information on key genetic resources for the maize community in the development of new improved lines.
25966324	0	44	theme	maize	33:37	arg1	populations					39:49	key maize populations	29:49	key maize populations	29:49	Compositional assessments of key maize populations: B73 hybrids of the Nested Association Mapping founder lines and diverse landrace inbred lines.
25966324	4	45	theme	acids	926:930	arg1	measurements					842:853	measurements	842:853	measurements of proximates (protein, fat, ash, and starch), fibers, minerals, amino acids, fatty acids, tocopherols (α-, γ-, and δ-), β-carotene, phytic acid, and raffinose	842:1013	The compositional analysis of B73 hybrids from the 25 NAM founders and 24 inbred lines derived from landraces included measurements of proximates (protein, fat, ash, and starch), fibers, minerals, amino acids, fatty acids, tocopherols (α-, γ-, and δ-), β-carotene, phytic acid, and raffinose.
25966324	3	46	theme	maize	603:607	arg1	community					609:617	the maize community	599:617	the maize community as they offer the potential to discover new alleles that could be incorporated into modern maize lines	599:720	Landraces are also of interest to the maize community as they offer the potential to discover new alleles that could be incorporated into modern maize lines.
25966324	1	47	theme	founder	295:301	arg1	lines					303:307	the Nested Association Mapping (NAM) founder lines	258:307	the Nested Association Mapping (NAM) founder lines	258:307	The present study provides an assessment of the compositional diversity in maize B73 hybrids derived both from the Nested Association Mapping (NAM) founder lines and from a diverse collection of landrace accessions from North and South America.
25966324	4	48	theme	B73	753:755	arg1	hybrids					757:763	B73 hybrids	753:763	B73 hybrids from the 25 NAM founders and 24 inbred lines derived from landraces	753:831	The compositional analysis of B73 hybrids from the 25 NAM founders and 24 inbred lines derived from landraces included measurements of proximates (protein, fat, ash, and starch), fibers, minerals, amino acids, fatty acids, tocopherols (α-, γ-, and δ-), β-carotene, phytic acid, and raffinose.
25966324	2	49	used	used	473:476	arg2	lines					458:462	publicly available lines	439:462	publicly available lines that are used extensively in the maize community to investigate the genetic basis of complex traits	439:562	The NAM founders represent a key population of publicly available lines that are used extensively in the maize community to investigate the genetic basis of complex traits.
25966324	3	50	dep	community	609:617	arg1	offer					627:631	offer	627:631	offer the potential to discover new alleles that could be incorporated into modern maize lines	627:720	Landraces are also of interest to the maize community as they offer the potential to discover new alleles that could be incorporated into modern maize lines.
25966324	9	51	theme	key	1688:1690	arg1	resources					1700:1708	key genetic resources	1688:1708	key genetic resources for the maize community in the development of new improved lines	1688:1773	The study described here provided significant information on contributions of conventional breeding to crop compositional variation, as well as valuable information on key genetic resources for the maize community in the development of new improved lines.
25966324	8	52	theme	maize	1507:1511	arg1	lines					1513:1517	modern maize lines	1500:1517	modern maize lines	1500:1517	The variation observed for some components within the landraces may therefore be of value for increasing their levels in modern maize lines.
25966324	6	53	dep	set	1092:1094	arg1	landrace					1105:1112	landrace	1105:1112	landrace	1105:1112	For each data set (NAM and landrace) canonical discriminant analysis allowed separation of distinct breeding groups (tropical, temperate, flint, mixed/intermediate) within each data set.
25966324	6	53	dep	set	1092:1094	arg1	NAM					1097:1099	NAM	1097:1099	NAM	1097:1099	For each data set (NAM and landrace) canonical discriminant analysis allowed separation of distinct breeding groups (tropical, temperate, flint, mixed/intermediate) within each data set.
25966324	9	54	from	information	1673:1683	arg1	resources					1700:1708	key genetic resources	1688:1708	key genetic resources for the maize community in the development of new improved lines	1688:1773	The study described here provided significant information on contributions of conventional breeding to crop compositional variation, as well as valuable information on key genetic resources for the maize community in the development of new improved lines.
25966324	9	54	from	information	1673:1683	arg1	contributions					1581:1593	contributions	1581:1593	contributions of conventional breeding to crop compositional variation	1581:1650	The study described here provided significant information on contributions of conventional breeding to crop compositional variation, as well as valuable information on key genetic resources for the maize community in the development of new improved lines.
25966324	5	55	theme	New	1063:1065	arg1	USA					1073:1075	USA	1073:1075	USA	1073:1075	Grain was harvested from a replicated trial in New York, USA.
25966324	5	55	theme	New	1063:1065	arg1	York					1067:1070	New York	1063:1070	New York	1063:1070	Grain was harvested from a replicated trial in New York, USA.
25966324	2	56	theme	traits	557:562	arg1	basis					540:544	the genetic basis	528:544	the genetic basis of complex traits	528:562	The NAM founders represent a key population of publicly available lines that are used extensively in the maize community to investigate the genetic basis of complex traits.
25966324	4	57	theme	acid	995:998	arg1	measurements					842:853	measurements	842:853	measurements of proximates (protein, fat, ash, and starch), fibers, minerals, amino acids, fatty acids, tocopherols (α-, γ-, and δ-), β-carotene, phytic acid, and raffinose	842:1013	The compositional analysis of B73 hybrids from the 25 NAM founders and 24 inbred lines derived from landraces included measurements of proximates (protein, fat, ash, and starch), fibers, minerals, amino acids, fatty acids, tocopherols (α-, γ-, and δ-), β-carotene, phytic acid, and raffinose.
25966324	9	58	theme	genetic	1692:1698	arg1	resources					1700:1708	key genetic resources	1688:1708	key genetic resources for the maize community in the development of new improved lines	1688:1773	The study described here provided significant information on contributions of conventional breeding to crop compositional variation, as well as valuable information on key genetic resources for the maize community in the development of new improved lines.
25966324	9	59	theme	improved	1760:1767	arg1	lines					1769:1773	new improved lines	1756:1773	new improved lines	1756:1773	The study described here provided significant information on contributions of conventional breeding to crop compositional variation, as well as valuable information on key genetic resources for the maize community in the development of new improved lines.
25966324	4	60	theme	fibers	902:907	arg1	measurements					842:853	measurements	842:853	measurements of proximates (protein, fat, ash, and starch), fibers, minerals, amino acids, fatty acids, tocopherols (α-, γ-, and δ-), β-carotene, phytic acid, and raffinose	842:1013	The compositional analysis of B73 hybrids from the 25 NAM founders and 24 inbred lines derived from landraces included measurements of proximates (protein, fat, ash, and starch), fibers, minerals, amino acids, fatty acids, tocopherols (α-, γ-, and δ-), β-carotene, phytic acid, and raffinose.
25966324	6	61	theme	data	1087:1090	arg1	set					1092:1094	each data set	1082:1094	each data set (NAM and landrace)	1082:1113	For each data set (NAM and landrace) canonical discriminant analysis allowed separation of distinct breeding groups (tropical, temperate, flint, mixed/intermediate) within each data set.
25966324	4	62	theme	inbred	797:802	arg1	lines					804:808	24 inbred lines	794:808	24 inbred lines derived from landraces	794:831	The compositional analysis of B73 hybrids from the 25 NAM founders and 24 inbred lines derived from landraces included measurements of proximates (protein, fat, ash, and starch), fibers, minerals, amino acids, fatty acids, tocopherols (α-, γ-, and δ-), β-carotene, phytic acid, and raffinose.
25966324	6	63	theme	breeding	1178:1185	arg1	groups					1187:1192	distinct breeding groups	1169:1192	distinct breeding groups (tropical, temperate, flint, mixed/intermediate)	1169:1241	For each data set (NAM and landrace) canonical discriminant analysis allowed separation of distinct breeding groups (tropical, temperate, flint, mixed/intermediate) within each data set.
25966324	3	64	theme	modern	703:708	arg1	lines					716:720	modern maize lines	703:720	modern maize lines	703:720	Landraces are also of interest to the maize community as they offer the potential to discover new alleles that could be incorporated into modern maize lines.
25966324	0	65	theme	inbred	133:138	arg1	lines					140:144	diverse landrace inbred lines	116:144	diverse landrace inbred lines	116:144	Compositional assessments of key maize populations: B73 hybrids of the Nested Association Mapping founder lines and diverse landrace inbred lines.
25966324	1	66	theme	compositional	195:207	arg1	diversity					209:217	the compositional diversity	191:217	the compositional diversity in maize B73 hybrids derived both from the Nested Association Mapping (NAM) founder lines and from a diverse collection of landrace accessions from North and South America	191:389	The present study provides an assessment of the compositional diversity in maize B73 hybrids derived both from the Nested Association Mapping (NAM) founder lines and from a diverse collection of landrace accessions from North and South America.
25966324	9	67	theme	crop	1623:1626	arg1	variation					1642:1650	crop compositional variation	1623:1650	crop compositional variation	1623:1650	The study described here provided significant information on contributions of conventional breeding to crop compositional variation, as well as valuable information on key genetic resources for the maize community in the development of new improved lines.
25966324	1	68	from	diversity	209:217	arg1	hybrids					232:238	maize B73 hybrids	222:238	maize B73 hybrids derived both from the Nested Association Mapping (NAM) founder lines and from a diverse collection of landrace accessions from North and South America	222:389	The present study provides an assessment of the compositional diversity in maize B73 hybrids derived both from the Nested Association Mapping (NAM) founder lines and from a diverse collection of landrace accessions from North and South America.
25966324	0	69	theme	diverse	116:122	arg1	lines					140:144	diverse landrace inbred lines	116:144	diverse landrace inbred lines	116:144	Compositional assessments of key maize populations: B73 hybrids of the Nested Association Mapping founder lines and diverse landrace inbred lines.
25966324	6	70	theme	discriminant	1125:1136	arg1	analysis					1138:1145	canonical discriminant analysis	1115:1145	canonical discriminant analysis	1115:1145	For each data set (NAM and landrace) canonical discriminant analysis allowed separation of distinct breeding groups (tropical, temperate, flint, mixed/intermediate) within each data set.
25966324	4	71	theme	proximates	858:867	arg1	measurements					842:853	measurements	842:853	measurements of proximates (protein, fat, ash, and starch), fibers, minerals, amino acids, fatty acids, tocopherols (α-, γ-, and δ-), β-carotene, phytic acid, and raffinose	842:1013	The compositional analysis of B73 hybrids from the 25 NAM founders and 24 inbred lines derived from landraces included measurements of proximates (protein, fat, ash, and starch), fibers, minerals, amino acids, fatty acids, tocopherols (α-, γ-, and δ-), β-carotene, phytic acid, and raffinose.
25966324	1	72	from	assessment	177:186	arg1	hybrids					232:238	maize B73 hybrids	222:238	maize B73 hybrids derived both from the Nested Association Mapping (NAM) founder lines and from a diverse collection of landrace accessions from North and South America	222:389	The present study provides an assessment of the compositional diversity in maize B73 hybrids derived both from the Nested Association Mapping (NAM) founder lines and from a diverse collection of landrace accessions from North and South America.
25966324	9	73	theme	conventional	1598:1609	arg1	breeding					1611:1618	conventional breeding	1598:1618	conventional breeding	1598:1618	The study described here provided significant information on contributions of conventional breeding to crop compositional variation, as well as valuable information on key genetic resources for the maize community in the development of new improved lines.
25966324	6	74	theme	tropical	1195:1202	arg1	mixed/intermediate					1223:1240	mixed/intermediate	1223:1240	mixed/intermediate	1223:1240	For each data set (NAM and landrace) canonical discriminant analysis allowed separation of distinct breeding groups (tropical, temperate, flint, mixed/intermediate) within each data set.
25966324	6	74	theme	tropical	1195:1202	arg1	flint					1216:1220	flint	1216:1220	flint	1216:1220	For each data set (NAM and landrace) canonical discriminant analysis allowed separation of distinct breeding groups (tropical, temperate, flint, mixed/intermediate) within each data set.
25966324	0	75	theme	Nested	71:76	arg1	Association					78:88	the Nested Association	67:88	the Nested Association Mapping founder lines and diverse landrace inbred lines	67:144	Compositional assessments of key maize populations: B73 hybrids of the Nested Association Mapping founder lines and diverse landrace inbred lines.
25966324	2	76	theme	lines	458:462	arg1	population					425:434	a key population	419:434	a key population of publicly available lines that are used extensively in the maize community to investigate the genetic basis of complex traits	419:562	The NAM founders represent a key population of publicly available lines that are used extensively in the maize community to investigate the genetic basis of complex traits.
25966324	1	77	theme	diverse	320:326	arg1	collection					328:337	a diverse collection	318:337	a diverse collection of landrace accessions	318:360	The present study provides an assessment of the compositional diversity in maize B73 hybrids derived both from the Nested Association Mapping (NAM) founder lines and from a diverse collection of landrace accessions from North and South America.
25966324	9	78	theme	compositional	1628:1640	arg1	variation					1642:1650	crop compositional variation	1623:1650	crop compositional variation	1623:1650	The study described here provided significant information on contributions of conventional breeding to crop compositional variation, as well as valuable information on key genetic resources for the maize community in the development of new improved lines.
25966324	4	79	dep	tocopherols	946:956	arg1	γ-					963:964	γ-	963:964	γ-	963:964	The compositional analysis of B73 hybrids from the 25 NAM founders and 24 inbred lines derived from landraces included measurements of proximates (protein, fat, ash, and starch), fibers, minerals, amino acids, fatty acids, tocopherols (α-, γ-, and δ-), β-carotene, phytic acid, and raffinose.
25966324	4	79	dep	tocopherols	946:956	arg1	δ-					971:972	δ-	971:972	δ-	971:972	The compositional analysis of B73 hybrids from the 25 NAM founders and 24 inbred lines derived from landraces included measurements of proximates (protein, fat, ash, and starch), fibers, minerals, amino acids, fatty acids, tocopherols (α-, γ-, and δ-), β-carotene, phytic acid, and raffinose.
25966324	4	80	theme	compositional	727:739	arg1	analysis					741:748	The compositional analysis	723:748	The compositional analysis of B73 hybrids from the 25 NAM founders and 24 inbred lines derived from landraces	723:831	The compositional analysis of B73 hybrids from the 25 NAM founders and 24 inbred lines derived from landraces included measurements of proximates (protein, fat, ash, and starch), fibers, minerals, amino acids, fatty acids, tocopherols (α-, γ-, and δ-), β-carotene, phytic acid, and raffinose.
25966324	1	81	theme	accessions	351:360	arg1	collection					328:337	a diverse collection	318:337	a diverse collection of landrace accessions	318:360	The present study provides an assessment of the compositional diversity in maize B73 hybrids derived both from the Nested Association Mapping (NAM) founder lines and from a diverse collection of landrace accessions from North and South America.
25966324	5	82	from	trial	1054:1058	arg1	USA					1073:1075	USA	1073:1075	USA	1073:1075	Grain was harvested from a replicated trial in New York, USA.
25966324	5	82	from	trial	1054:1058	arg1	York					1067:1070	New York	1063:1070	New York	1063:1070	Grain was harvested from a replicated trial in New York, USA.
25966324	0	83	theme	key	29:31	arg1	populations					39:49	key maize populations	29:49	key maize populations	29:49	Compositional assessments of key maize populations: B73 hybrids of the Nested Association Mapping founder lines and diverse landrace inbred lines.
25966324	1	84	theme	Association	269:279	arg1	Mapping					281:287	the Nested Association Mapping	258:287	the Nested Association Mapping (NAM) founder lines	258:307	The present study provides an assessment of the compositional diversity in maize B73 hybrids derived both from the Nested Association Mapping (NAM) founder lines and from a diverse collection of landrace accessions from North and South America.
25966324	1	84	theme	Association	269:279	arg1	NAM					290:292	NAM	290:292	NAM	290:292	The present study provides an assessment of the compositional diversity in maize B73 hybrids derived both from the Nested Association Mapping (NAM) founder lines and from a diverse collection of landrace accessions from North and South America.
25966324	4	85	theme	acids	939:943	arg1	measurements					842:853	measurements	842:853	measurements of proximates (protein, fat, ash, and starch), fibers, minerals, amino acids, fatty acids, tocopherols (α-, γ-, and δ-), β-carotene, phytic acid, and raffinose	842:1013	The compositional analysis of B73 hybrids from the 25 NAM founders and 24 inbred lines derived from landraces included measurements of proximates (protein, fat, ash, and starch), fibers, minerals, amino acids, fatty acids, tocopherols (α-, γ-, and δ-), β-carotene, phytic acid, and raffinose.
25966324	0	86	theme	populations	39:49	arg1	assessments					14:24	Compositional assessments	0:24	Compositional assessments of key maize populations: B73 hybrids of the Nested Association Mapping founder lines and diverse landrace inbred lines.	0:145	Compositional assessments of key maize populations: B73 hybrids of the Nested Association Mapping founder lines and diverse landrace inbred lines.
25966324	5	87	theme	replicated	1043:1052	arg1	trial					1054:1058	a replicated trial	1041:1058	a replicated trial in New York, USA	1041:1075	Grain was harvested from a replicated trial in New York, USA.
25966324	4	88	theme	minerals	910:917	arg1	measurements					842:853	measurements	842:853	measurements of proximates (protein, fat, ash, and starch), fibers, minerals, amino acids, fatty acids, tocopherols (α-, γ-, and δ-), β-carotene, phytic acid, and raffinose	842:1013	The compositional analysis of B73 hybrids from the 25 NAM founders and 24 inbred lines derived from landraces included measurements of proximates (protein, fat, ash, and starch), fibers, minerals, amino acids, fatty acids, tocopherols (α-, γ-, and δ-), β-carotene, phytic acid, and raffinose.
25966324	1	89	theme	B73	228:230	arg1	hybrids					232:238	maize B73 hybrids	222:238	maize B73 hybrids derived both from the Nested Association Mapping (NAM) founder lines and from a diverse collection of landrace accessions from North and South America	222:389	The present study provides an assessment of the compositional diversity in maize B73 hybrids derived both from the Nested Association Mapping (NAM) founder lines and from a diverse collection of landrace accessions from North and South America.
25966324	6	90	theme	groups	1187:1192	arg1	separation					1155:1164	separation	1155:1164	separation of distinct breeding groups (tropical, temperate, flint, mixed/intermediate)	1155:1241	For each data set (NAM and landrace) canonical discriminant analysis allowed separation of distinct breeding groups (tropical, temperate, flint, mixed/intermediate) within each data set.
25966324	0	91	theme	B73	52:54	arg1	hybrids					56:62	B73 hybrids	52:62	Compositional assessments of key maize populations: B73 hybrids of the Nested Association Mapping founder lines and diverse landrace inbred lines.	0:145	Compositional assessments of key maize populations: B73 hybrids of the Nested Association Mapping founder lines and diverse landrace inbred lines.
25966324	4	92	theme	amino	920:924	arg1	acids					926:930	amino acids	920:930	amino acids	920:930	The compositional analysis of B73 hybrids from the 25 NAM founders and 24 inbred lines derived from landraces included measurements of proximates (protein, fat, ash, and starch), fibers, minerals, amino acids, fatty acids, tocopherols (α-, γ-, and δ-), β-carotene, phytic acid, and raffinose.
25966324	4	93	theme	NAM	777:779	arg1	founders					781:788	the 25 NAM founders	770:788	the 25 NAM founders	770:788	The compositional analysis of B73 hybrids from the 25 NAM founders and 24 inbred lines derived from landraces included measurements of proximates (protein, fat, ash, and starch), fibers, minerals, amino acids, fatty acids, tocopherols (α-, γ-, and δ-), β-carotene, phytic acid, and raffinose.
25966324	8	94	theme	modern	1500:1505	arg1	lines					1513:1517	modern maize lines	1500:1517	modern maize lines	1500:1517	The variation observed for some components within the landraces may therefore be of value for increasing their levels in modern maize lines.
25966324	1	95	theme	maize	222:226	arg1	hybrids					232:238	maize B73 hybrids	222:238	maize B73 hybrids derived both from the Nested Association Mapping (NAM) founder lines and from a diverse collection of landrace accessions from North and South America	222:389	The present study provides an assessment of the compositional diversity in maize B73 hybrids derived both from the Nested Association Mapping (NAM) founder lines and from a diverse collection of landrace accessions from North and South America.
25966324	0	96	dep	assessments	14:24	arg1	hybrids					56:62	B73 hybrids	52:62	Compositional assessments of key maize populations: B73 hybrids of the Nested Association Mapping founder lines and diverse landrace inbred lines.	0:145	Compositional assessments of key maize populations: B73 hybrids of the Nested Association Mapping founder lines and diverse landrace inbred lines.
25966324	9	97	theme	maize	1718:1722	arg1	community					1724:1732	the maize community	1714:1732	the maize community in the development of new improved lines	1714:1773	The study described here provided significant information on contributions of conventional breeding to crop compositional variation, as well as valuable information on key genetic resources for the maize community in the development of new improved lines.
25966324	3	98	theme	new	659:661	arg1	alleles					663:669	new alleles	659:669	new alleles that could be incorporated into modern maize lines	659:720	Landraces are also of interest to the maize community as they offer the potential to discover new alleles that could be incorporated into modern maize lines.
25966324	4	99	theme	hybrids	757:763	arg1	analysis					741:748	The compositional analysis	723:748	The compositional analysis of B73 hybrids from the 25 NAM founders and 24 inbred lines derived from landraces	723:831	The compositional analysis of B73 hybrids from the 25 NAM founders and 24 inbred lines derived from landraces included measurements of proximates (protein, fat, ash, and starch), fibers, minerals, amino acids, fatty acids, tocopherols (α-, γ-, and δ-), β-carotene, phytic acid, and raffinose.
25966324	4	100	theme	fatty	933:937	arg1	acids					939:943	fatty acids	933:943	fatty acids	933:943	The compositional analysis of B73 hybrids from the 25 NAM founders and 24 inbred lines derived from landraces included measurements of proximates (protein, fat, ash, and starch), fibers, minerals, amino acids, fatty acids, tocopherols (α-, γ-, and δ-), β-carotene, phytic acid, and raffinose.
26454167	1	0	theme	Various	96:102	arg1	thiosaccharides					104:118	Various thiosaccharides	96:118	Various thiosaccharides	96:118	Various thiosaccharides and 1-thiotrehaloses were synthesized exploiting salient features of gold-catalyzed glycosidation repertoire using alkynyl 1,2-orthoesters as glycosyl donors.
26454167	1	1	theme	salient	169:175	arg1	features					177:184	salient features	169:184	salient features of gold-catalyzed glycosidation repertoire	169:227	Various thiosaccharides and 1-thiotrehaloses were synthesized exploiting salient features of gold-catalyzed glycosidation repertoire using alkynyl 1,2-orthoesters as glycosyl donors.
26454167	1	2	theme	alkynyl	235:241	arg1	1,2-orthoesters					243:257	alkynyl 1,2-orthoesters	235:257	alkynyl 1,2-orthoesters	235:257	Various thiosaccharides and 1-thiotrehaloses were synthesized exploiting salient features of gold-catalyzed glycosidation repertoire using alkynyl 1,2-orthoesters as glycosyl donors.
26454167	1	2	theme	alkynyl	235:241	arg1	donors					271:276	glycosyl donors	262:276	glycosyl donors	262:276	Various thiosaccharides and 1-thiotrehaloses were synthesized exploiting salient features of gold-catalyzed glycosidation repertoire using alkynyl 1,2-orthoesters as glycosyl donors.
26454167	1	3	theme	repertoire	218:227	arg1	features					177:184	salient features	169:184	salient features of gold-catalyzed glycosidation repertoire	169:227	Various thiosaccharides and 1-thiotrehaloses were synthesized exploiting salient features of gold-catalyzed glycosidation repertoire using alkynyl 1,2-orthoesters as glycosyl donors.
26454167	2	4	dep	general	308:314	arg1	mild					358:361	mild	358:361	mild	358:361	The reaction was found to be general, high yielding, diastereoselective, fast, mild and catalytic.
26454167	2	4	dep	general	308:314	arg1	catalytic					367:375	catalytic	367:375	catalytic	367:375	The reaction was found to be general, high yielding, diastereoselective, fast, mild and catalytic.
26454167	2	4	dep	general	308:314	arg1	diastereoselective					332:349	diastereoselective	332:349	diastereoselective	332:349	The reaction was found to be general, high yielding, diastereoselective, fast, mild and catalytic.
26454167	2	4	dep	general	308:314	arg1	fast					352:355	fast	352:355	fast	352:355	The reaction was found to be general, high yielding, diastereoselective, fast, mild and catalytic.
26454167	2	4	dep	general	308:314	arg1	general					308:314	general	308:314	general	308:314	The reaction was found to be general, high yielding, diastereoselective, fast, mild and catalytic.
26454167	2	4	dep	general	308:314	arg1	reaction					283:290	The reaction	279:290	The reaction	279:290	The reaction was found to be general, high yielding, diastereoselective, fast, mild and catalytic.
26454167	2	4	dep	general	308:314	arg1	yielding					322:329	yielding	322:329	yielding	322:329	The reaction was found to be general, high yielding, diastereoselective, fast, mild and catalytic.
26454167	2	5	dep	yielding	322:329	arg1	high					317:320	high	317:320	high	317:320	The reaction was found to be general, high yielding, diastereoselective, fast, mild and catalytic.
26454167	1	6	theme	gold-catalyzed	189:202	arg1	repertoire					218:227	gold-catalyzed glycosidation repertoire	189:227	gold-catalyzed glycosidation repertoire	189:227	Various thiosaccharides and 1-thiotrehaloses were synthesized exploiting salient features of gold-catalyzed glycosidation repertoire using alkynyl 1,2-orthoesters as glycosyl donors.
26454167	1	7	theme	glycosyl	262:269	arg1	1,2-orthoesters					243:257	alkynyl 1,2-orthoesters	235:257	alkynyl 1,2-orthoesters	235:257	Various thiosaccharides and 1-thiotrehaloses were synthesized exploiting salient features of gold-catalyzed glycosidation repertoire using alkynyl 1,2-orthoesters as glycosyl donors.
26454167	1	7	theme	glycosyl	262:269	arg1	donors					271:276	glycosyl donors	262:276	glycosyl donors	262:276	Various thiosaccharides and 1-thiotrehaloses were synthesized exploiting salient features of gold-catalyzed glycosidation repertoire using alkynyl 1,2-orthoesters as glycosyl donors.
26454167	0	8	theme	stereoselective	30:44	arg1	synthesis					46:54	stereoselective synthesis	30:54	stereoselective synthesis of thioglycosides and 1-thiotrehaloses	30:93	Propargyl 1,2-orthoesters for stereoselective synthesis of thioglycosides and 1-thiotrehaloses.
26454167	0	9	theme	1-thiotrehaloses	78:93	arg1	synthesis					46:54	stereoselective synthesis	30:54	stereoselective synthesis of thioglycosides and 1-thiotrehaloses	30:93	Propargyl 1,2-orthoesters for stereoselective synthesis of thioglycosides and 1-thiotrehaloses.
26454167	0	10	theme	thioglycosides	59:72	arg1	synthesis					46:54	stereoselective synthesis	30:54	stereoselective synthesis of thioglycosides and 1-thiotrehaloses	30:93	Propargyl 1,2-orthoesters for stereoselective synthesis of thioglycosides and 1-thiotrehaloses.
26454167	1	11	theme	glycosidation	204:216	arg1	repertoire					218:227	gold-catalyzed glycosidation repertoire	189:227	gold-catalyzed glycosidation repertoire	189:227	Various thiosaccharides and 1-thiotrehaloses were synthesized exploiting salient features of gold-catalyzed glycosidation repertoire using alkynyl 1,2-orthoesters as glycosyl donors.
25171516	12	0	theme	sulfate-containing	1418:1435	arg1	copolymers					1443:1452	The chondroitin sulfate-containing graft copolymers	1402:1452	The chondroitin sulfate-containing graft copolymers	1402:1452	The chondroitin sulfate-containing graft copolymers successfully deliver FGF-2 to cells from surfaces.
25171516	14	1	theme	graft	1721:1725	arg1	density					1727:1733	graft density	1721:1733	graft density	1721:1733	This new method for preparation of GAG-based graft copolymers enables a wide range of graft density, and can be used to explore applications of PG mimics as new biomaterials with tunable biochemical and biomechanical functions.
25171516	8	2	theme	graft	947:951	arg1	copolymers					953:962	graft copolymers	947:962	graft copolymers with a wide range of sizes	947:989	PG mimics with heparin or chondroitin sulfate side chains and four different ratios of GAG side chain result in graft copolymers with a wide range of sizes.
25171516	14	3	with	biomaterials	1796:1807	arg1	functions					1852:1860	tunable biochemical and biomechanical functions	1814:1860	tunable biochemical and biomechanical functions	1814:1860	This new method for preparation of GAG-based graft copolymers enables a wide range of graft density, and can be used to explore applications of PG mimics as new biomaterials with tunable biochemical and biomechanical functions.
25171516	13	4	theme	growth	1596:1601	arg1	factor					1603:1608	growth factor	1596:1608	growth factor delivery from a surface	1596:1632	The lowest graft density of heparin-containing PG mimic also performs well with respect to growth factor delivery from a surface.
25171516	14	5	theme	density	1727:1733	arg1	range					1712:1716	a wide range	1705:1716	a wide range of graft density	1705:1733	This new method for preparation of GAG-based graft copolymers enables a wide range of graft density, and can be used to explore applications of PG mimics as new biomaterials with tunable biochemical and biomechanical functions.
25171516	2	6	theme	glycosaminoglycan	267:283	arg1	chains					296:301	glycosaminoglycan (GAG) side chains	267:301	glycosaminoglycan (GAG) side chains	267:301	They are made up of a core protein with glycosaminoglycan (GAG) side chains.
25171516	8	7	theme	wide	971:974	arg1	range					976:980	a wide range	969:980	a wide range of sizes	969:989	PG mimics with heparin or chondroitin sulfate side chains and four different ratios of GAG side chain result in graft copolymers with a wide range of sizes.
25171516	11	8	theme	basic	1329:1333	arg1	FGF-2					1361:1365	FGF-2	1361:1365	FGF-2	1361:1365	Graft copolymers were tested for their ability to bind and deliver basic fibroblast growth factor (FGF-2) to mesenchymal stem cells (MSCs).
25171516	11	8	theme	basic	1329:1333	arg1	factor					1353:1358	basic fibroblast growth factor	1329:1358	basic fibroblast growth factor (FGF-2)	1329:1366	Graft copolymers were tested for their ability to bind and deliver basic fibroblast growth factor (FGF-2) to mesenchymal stem cells (MSCs).
25171516	5	9	theme	chondroitin	608:618	arg1	sulfate					620:626	chondroitin sulfate	608:626	chondroitin sulfate	608:626	This is done using three different GAGs: hyaluronan, chondroitin sulfate, and heparin.
25171516	5	9	theme	chondroitin	608:618	arg1	GAGs					590:593	three different GAGs	574:593	three different GAGs: hyaluronan, chondroitin sulfate, and heparin	574:639	This is done using three different GAGs: hyaluronan, chondroitin sulfate, and heparin.
25171516	7	10	theme	reductive	814:822	arg1	amination					824:832	reductive amination	814:832	reductive amination	814:832	Either chondroitin sulfate or heparin is grafted by the reducing end on to the hyaluronan backbone through reductive amination.
25171516	12	11	from	surfaces	1495:1502	arg1	cells					1484:1488	cells	1484:1488	cells from surfaces	1484:1502	The chondroitin sulfate-containing graft copolymers successfully deliver FGF-2 to cells from surfaces.
25171516	14	12	theme	wide	1707:1710	arg1	range					1712:1716	a wide range	1705:1716	a wide range of graft density	1705:1733	This new method for preparation of GAG-based graft copolymers enables a wide range of graft density, and can be used to explore applications of PG mimics as new biomaterials with tunable biochemical and biomechanical functions.
25171516	2	13	theme	side	291:294	arg1	chains					296:301	glycosaminoglycan (GAG) side chains	267:301	glycosaminoglycan (GAG) side chains	267:301	They are made up of a core protein with glycosaminoglycan (GAG) side chains.
25171516	14	14	theme	biomechanical	1838:1850	arg1	functions					1852:1860	tunable biochemical and biomechanical functions	1814:1860	tunable biochemical and biomechanical functions	1814:1860	This new method for preparation of GAG-based graft copolymers enables a wide range of graft density, and can be used to explore applications of PG mimics as new biomaterials with tunable biochemical and biomechanical functions.
25171516	14	15	dep	PG	1779:1780	arg1	mimics					1782:1787	mimics	1782:1787	mimics	1782:1787	This new method for preparation of GAG-based graft copolymers enables a wide range of graft density, and can be used to explore applications of PG mimics as new biomaterials with tunable biochemical and biomechanical functions.
25171516	1	16	gly	glycosylated	146:157	arg1	proteins					159:166	important glycosylated proteins	136:166	important glycosylated proteins found on the cell surface and in the extracellular matrix	136:224	Proteoglycans (PGs) are important glycosylated proteins found on the cell surface and in the extracellular matrix.
25171516	1	16	gly	glycosylated	146:157	arg1	Proteoglycans					112:124	Proteoglycans	112:124	Proteoglycans (PGs)	112:130	Proteoglycans (PGs) are important glycosylated proteins found on the cell surface and in the extracellular matrix.
25171516	13	17	theme	PG	1552:1553	arg1	density					1522:1528	The lowest graft density	1505:1528	The lowest graft density of heparin-containing PG	1505:1553	The lowest graft density of heparin-containing PG mimic also performs well with respect to growth factor delivery from a surface.
25171516	11	18	theme	growth	1346:1351	arg1	FGF-2					1361:1365	FGF-2	1361:1365	FGF-2	1361:1365	Graft copolymers were tested for their ability to bind and deliver basic fibroblast growth factor (FGF-2) to mesenchymal stem cells (MSCs).
25171516	11	18	theme	growth	1346:1351	arg1	factor					1353:1358	basic fibroblast growth factor	1329:1358	basic fibroblast growth factor (FGF-2)	1329:1366	Graft copolymers were tested for their ability to bind and deliver basic fibroblast growth factor (FGF-2) to mesenchymal stem cells (MSCs).
25171516	14	19	theme	graft	1680:1684	arg1	copolymers					1686:1695	GAG-based graft copolymers	1670:1695	GAG-based graft copolymers	1670:1695	This new method for preparation of GAG-based graft copolymers enables a wide range of graft density, and can be used to explore applications of PG mimics as new biomaterials with tunable biochemical and biomechanical functions.
25171516	1	20	theme	important	136:144	arg1	proteins					159:166	important glycosylated proteins	136:166	important glycosylated proteins found on the cell surface and in the extracellular matrix	136:224	Proteoglycans (PGs) are important glycosylated proteins found on the cell surface and in the extracellular matrix.
25171516	1	20	theme	important	136:144	arg1	Proteoglycans					112:124	Proteoglycans	112:124	Proteoglycans (PGs)	112:130	Proteoglycans (PGs) are important glycosylated proteins found on the cell surface and in the extracellular matrix.
25171516	2	21	with	protein	254:260	arg1	chains					296:301	glycosaminoglycan (GAG) side chains	267:301	glycosaminoglycan (GAG) side chains	267:301	They are made up of a core protein with glycosaminoglycan (GAG) side chains.
25171516	10	22	theme	scattering	1208:1217	arg1	measurements					1248:1259	dynamic light scattering and electrophoretic mobility measurements	1194:1259	dynamic light scattering and electrophoretic mobility measurements	1194:1259	Effective hydrodynamic diameter and zeta potential are determined using dynamic light scattering and electrophoretic mobility measurements.
25171516	13	23	theme	graft	1516:1520	arg1	density					1522:1528	The lowest graft density	1505:1528	The lowest graft density of heparin-containing PG	1505:1553	The lowest graft density of heparin-containing PG mimic also performs well with respect to growth factor delivery from a surface.
25171516	14	24	theme	biochemical	1822:1832	arg1	functions					1852:1860	tunable biochemical and biomechanical functions	1814:1860	tunable biochemical and biomechanical functions	1814:1860	This new method for preparation of GAG-based graft copolymers enables a wide range of graft density, and can be used to explore applications of PG mimics as new biomaterials with tunable biochemical and biomechanical functions.
25171516	10	25	theme	dynamic	1194:1200	arg1	scattering					1208:1217	dynamic light scattering	1194:1217	dynamic light scattering	1194:1217	Effective hydrodynamic diameter and zeta potential are determined using dynamic light scattering and electrophoretic mobility measurements.
25171516	0	26	theme	tunable	77:83	arg1	density					103:109	tunable glycosaminoglycan density	77:109	tunable glycosaminoglycan density	77:109	Synthesis and characterization of proteoglycan-mimetic graft copolymers with tunable glycosaminoglycan density.
25171516	8	27	theme	PG	835:836	arg1	mimics					838:843	PG mimics	835:843	PG mimics with heparin or chondroitin sulfate side chains	835:891	PG mimics with heparin or chondroitin sulfate side chains and four different ratios of GAG side chain result in graft copolymers with a wide range of sizes.
25171516	8	28	with	copolymers	953:962	arg1	range					976:980	a wide range	969:980	a wide range of sizes	969:989	PG mimics with heparin or chondroitin sulfate side chains and four different ratios of GAG side chain result in graft copolymers with a wide range of sizes.
25171516	4	29	theme	proteoglycan-mimetic	454:473	arg1	copolymers					481:490	proteoglycan-mimetic graft copolymers	454:490	proteoglycan-mimetic graft copolymers (or neoproteoglycans)	454:512	Here we present a method for the synthesis of proteoglycan-mimetic graft copolymers (or neoproteoglycans) with tunable GAG side-chain composition.
25171516	4	29	theme	proteoglycan-mimetic	454:473	arg1	neoproteoglycans					496:511	neoproteoglycans	496:511	neoproteoglycans	496:511	Here we present a method for the synthesis of proteoglycan-mimetic graft copolymers (or neoproteoglycans) with tunable GAG side-chain composition.
25171516	14	30	theme	new	1640:1642	arg1	method					1644:1649	This new method	1635:1649	This new method for preparation of GAG-based graft copolymers	1635:1695	This new method for preparation of GAG-based graft copolymers enables a wide range of graft density, and can be used to explore applications of PG mimics as new biomaterials with tunable biochemical and biomechanical functions.
25171516	1	31	theme	cell	181:184	arg1	surface					186:192	the cell surface	177:192	the cell surface	177:192	Proteoglycans (PGs) are important glycosylated proteins found on the cell surface and in the extracellular matrix.
25171516	3	32	theme	side	348:351	arg1	chains					353:358	GAG side chains	344:358	GAG side chains	344:358	Variations in composition and number of GAG side chains lead to a vast array of PG sizes and functions.
25171516	4	33	theme	copolymers	481:490	arg1	synthesis					441:449	the synthesis	437:449	the synthesis of proteoglycan-mimetic graft copolymers (or neoproteoglycans) with tunable GAG side-chain composition	437:552	Here we present a method for the synthesis of proteoglycan-mimetic graft copolymers (or neoproteoglycans) with tunable GAG side-chain composition.
25171516	11	34	theme	Graft	1262:1266	arg1	copolymers					1268:1277	Graft copolymers	1262:1277	Graft copolymers	1262:1277	Graft copolymers were tested for their ability to bind and deliver basic fibroblast growth factor (FGF-2) to mesenchymal stem cells (MSCs).
25171516	9	35	theme	total	1039:1043	arg1	Fourier					1057:1063	attentuated total reflectance Fourier	1027:1063	attentuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) and (1)H NMR	1027:1119	The chemistry is confirmed through attentuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) and (1)H NMR.
25171516	5	36	theme	different	580:588	arg1	heparin					633:639	heparin	633:639	heparin	633:639	This is done using three different GAGs: hyaluronan, chondroitin sulfate, and heparin.
25171516	5	36	theme	different	580:588	arg1	hyaluronan					596:605	hyaluronan	596:605	hyaluronan	596:605	This is done using three different GAGs: hyaluronan, chondroitin sulfate, and heparin.
25171516	5	36	theme	different	580:588	arg1	sulfate					620:626	chondroitin sulfate	608:626	chondroitin sulfate	608:626	This is done using three different GAGs: hyaluronan, chondroitin sulfate, and heparin.
25171516	5	36	theme	different	580:588	arg1	GAGs					590:593	three different GAGs	574:593	three different GAGs: hyaluronan, chondroitin sulfate, and heparin	574:639	This is done using three different GAGs: hyaluronan, chondroitin sulfate, and heparin.
25171516	6	37	theme	hydrazide-presenting	678:697	arg1	linker					699:704	a hydrazide-presenting linker	676:704	a hydrazide-presenting linker	676:704	Hyaluronan is functionalized with a hydrazide-presenting linker.
25171516	8	38	with	ratios	912:917	arg1	heparin					850:856	heparin	850:856	heparin	850:856	PG mimics with heparin or chondroitin sulfate side chains and four different ratios of GAG side chain result in graft copolymers with a wide range of sizes.
25171516	8	38	with	ratios	912:917	arg1	sulfate					873:879	chondroitin sulfate	861:879	chondroitin sulfate	861:879	PG mimics with heparin or chondroitin sulfate side chains and four different ratios of GAG side chain result in graft copolymers with a wide range of sizes.
25171516	4	39	theme	tunable	519:525	arg1	composition					542:552	tunable GAG side-chain composition	519:552	tunable GAG side-chain composition	519:552	Here we present a method for the synthesis of proteoglycan-mimetic graft copolymers (or neoproteoglycans) with tunable GAG side-chain composition.
25171516	14	40	theme	PG	1779:1780	arg1	applications					1763:1774	applications	1763:1774	applications of PG mimics	1763:1787	This new method for preparation of GAG-based graft copolymers enables a wide range of graft density, and can be used to explore applications of PG mimics as new biomaterials with tunable biochemical and biomechanical functions.
25171516	14	40	theme	PG	1779:1780	arg1	biomaterials					1796:1807	new biomaterials	1792:1807	new biomaterials with tunable biochemical and biomechanical functions	1792:1860	This new method for preparation of GAG-based graft copolymers enables a wide range of graft density, and can be used to explore applications of PG mimics as new biomaterials with tunable biochemical and biomechanical functions.
25171516	3	41	theme	PG	384:385	arg1	sizes					387:391	PG sizes	384:391	PG sizes	384:391	Variations in composition and number of GAG side chains lead to a vast array of PG sizes and functions.
25171516	4	42	theme	side-chain	531:540	arg1	composition					542:552	tunable GAG side-chain composition	519:552	tunable GAG side-chain composition	519:552	Here we present a method for the synthesis of proteoglycan-mimetic graft copolymers (or neoproteoglycans) with tunable GAG side-chain composition.
25171516	0	43	theme	proteoglycan-mimetic	34:53	arg1	copolymers					61:70	proteoglycan-mimetic graft copolymers	34:70	proteoglycan-mimetic graft copolymers	34:70	Synthesis and characterization of proteoglycan-mimetic graft copolymers with tunable glycosaminoglycan density.
25171516	7	44	theme	reducing	763:770	arg1	end					772:774	the reducing end	759:774	the reducing end on to the hyaluronan backbone through reductive amination	759:832	Either chondroitin sulfate or heparin is grafted by the reducing end on to the hyaluronan backbone through reductive amination.
25171516	9	45	dep	transform	1065:1073	arg1	infrared					1075:1082	infrared	1075:1082	transform infrared spectroscopy (ATR-FTIR) and (1)H NMR	1065:1119	The chemistry is confirmed through attentuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) and (1)H NMR.
25171516	10	46	theme	Effective	1122:1130	arg1	diameter					1145:1152	Effective hydrodynamic diameter	1122:1152	Effective hydrodynamic diameter	1122:1152	Effective hydrodynamic diameter and zeta potential are determined using dynamic light scattering and electrophoretic mobility measurements.
25171516	0	47	with	characterization	14:29	arg1	density					103:109	tunable glycosaminoglycan density	77:109	tunable glycosaminoglycan density	77:109	Synthesis and characterization of proteoglycan-mimetic graft copolymers with tunable glycosaminoglycan density.
25171516	0	48	theme	copolymers	61:70	arg1	characterization					14:29	characterization	14:29	characterization	14:29	Synthesis and characterization of proteoglycan-mimetic graft copolymers with tunable glycosaminoglycan density.
25171516	0	48	theme	copolymers	61:70	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis and characterization of proteoglycan-mimetic graft copolymers with tunable glycosaminoglycan density.
25171516	8	49	theme	GAG	922:924	arg1	chain					931:935	GAG side chain	922:935	GAG side chain	922:935	PG mimics with heparin or chondroitin sulfate side chains and four different ratios of GAG side chain result in graft copolymers with a wide range of sizes.
25171516	0	50	with	Synthesis	0:8	arg1	density					103:109	tunable glycosaminoglycan density	77:109	tunable glycosaminoglycan density	77:109	Synthesis and characterization of proteoglycan-mimetic graft copolymers with tunable glycosaminoglycan density.
25171516	10	51	theme	zeta	1158:1161	arg1	potential					1163:1171	zeta potential	1158:1171	zeta potential	1158:1171	Effective hydrodynamic diameter and zeta potential are determined using dynamic light scattering and electrophoretic mobility measurements.
25171516	8	52	theme	chain	931:935	arg1	mimics					838:843	PG mimics	835:843	PG mimics with heparin or chondroitin sulfate side chains	835:891	PG mimics with heparin or chondroitin sulfate side chains and four different ratios of GAG side chain result in graft copolymers with a wide range of sizes.
25171516	8	52	theme	chain	931:935	arg1	ratios					912:917	four different ratios	897:917	four different ratios of GAG side chain	897:935	PG mimics with heparin or chondroitin sulfate side chains and four different ratios of GAG side chain result in graft copolymers with a wide range of sizes.
25171516	12	53	theme	graft	1437:1441	arg1	copolymers					1443:1452	The chondroitin sulfate-containing graft copolymers	1402:1452	The chondroitin sulfate-containing graft copolymers	1402:1452	The chondroitin sulfate-containing graft copolymers successfully deliver FGF-2 to cells from surfaces.
25171516	9	54	dep	spectroscopy	1084:1095	arg1	ATR-FTIR					1098:1105	ATR-FTIR	1098:1105	ATR-FTIR	1098:1105	The chemistry is confirmed through attentuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) and (1)H NMR.
25171516	7	55	theme	chondroitin	714:724	arg1	sulfate					726:732	chondroitin sulfate	714:732	chondroitin sulfate	714:732	Either chondroitin sulfate or heparin is grafted by the reducing end on to the hyaluronan backbone through reductive amination.
25171516	11	56	theme	stem	1383:1386	arg1	cells					1388:1392	mesenchymal stem cells	1371:1392	mesenchymal stem cells (MSCs)	1371:1399	Graft copolymers were tested for their ability to bind and deliver basic fibroblast growth factor (FGF-2) to mesenchymal stem cells (MSCs).
25171516	11	56	theme	stem	1383:1386	arg1	MSCs					1395:1398	MSCs	1395:1398	MSCs	1395:1398	Graft copolymers were tested for their ability to bind and deliver basic fibroblast growth factor (FGF-2) to mesenchymal stem cells (MSCs).
25171516	13	57	theme	factor	1603:1608	arg1	delivery					1610:1617	growth factor delivery	1596:1617	growth factor delivery from a surface	1596:1632	The lowest graft density of heparin-containing PG mimic also performs well with respect to growth factor delivery from a surface.
25171516	12	58	theme	chondroitin	1406:1416	arg1	copolymers					1443:1452	The chondroitin sulfate-containing graft copolymers	1402:1452	The chondroitin sulfate-containing graft copolymers	1402:1452	The chondroitin sulfate-containing graft copolymers successfully deliver FGF-2 to cells from surfaces.
25171516	14	59	used	used	1747:1750	arg2	method					1644:1649	This new method	1635:1649	This new method for preparation of GAG-based graft copolymers	1635:1695	This new method for preparation of GAG-based graft copolymers enables a wide range of graft density, and can be used to explore applications of PG mimics as new biomaterials with tunable biochemical and biomechanical functions.
25171516	8	60	theme	sizes	985:989	arg1	range					976:980	a wide range	969:980	a wide range of sizes	969:989	PG mimics with heparin or chondroitin sulfate side chains and four different ratios of GAG side chain result in graft copolymers with a wide range of sizes.
25171516	8	61	dep	heparin	850:856	arg1	chains					886:891	side chains	881:891	side chains	881:891	PG mimics with heparin or chondroitin sulfate side chains and four different ratios of GAG side chain result in graft copolymers with a wide range of sizes.
25171516	1	62	theme	extracellular	205:217	arg1	matrix					219:224	the extracellular matrix	201:224	the extracellular matrix	201:224	Proteoglycans (PGs) are important glycosylated proteins found on the cell surface and in the extracellular matrix.
25171516	14	63	theme	new	1792:1794	arg1	applications					1763:1774	applications	1763:1774	applications of PG mimics	1763:1787	This new method for preparation of GAG-based graft copolymers enables a wide range of graft density, and can be used to explore applications of PG mimics as new biomaterials with tunable biochemical and biomechanical functions.
25171516	14	63	theme	new	1792:1794	arg1	biomaterials					1796:1807	new biomaterials	1792:1807	new biomaterials with tunable biochemical and biomechanical functions	1792:1860	This new method for preparation of GAG-based graft copolymers enables a wide range of graft density, and can be used to explore applications of PG mimics as new biomaterials with tunable biochemical and biomechanical functions.
25171516	10	64	theme	mobility	1239:1246	arg1	measurements					1248:1259	dynamic light scattering and electrophoretic mobility measurements	1194:1259	dynamic light scattering and electrophoretic mobility measurements	1194:1259	Effective hydrodynamic diameter and zeta potential are determined using dynamic light scattering and electrophoretic mobility measurements.
25171516	13	65	theme	heparin-containing	1533:1550	arg1	PG					1552:1553	heparin-containing PG	1533:1553	heparin-containing PG	1533:1553	The lowest graft density of heparin-containing PG mimic also performs well with respect to growth factor delivery from a surface.
25171516	11	66	theme	fibroblast	1335:1344	arg1	FGF-2					1361:1365	FGF-2	1361:1365	FGF-2	1361:1365	Graft copolymers were tested for their ability to bind and deliver basic fibroblast growth factor (FGF-2) to mesenchymal stem cells (MSCs).
25171516	11	66	theme	fibroblast	1335:1344	arg1	factor					1353:1358	basic fibroblast growth factor	1329:1358	basic fibroblast growth factor (FGF-2)	1329:1366	Graft copolymers were tested for their ability to bind and deliver basic fibroblast growth factor (FGF-2) to mesenchymal stem cells (MSCs).
25171516	14	67	theme	copolymers	1686:1695	arg1	preparation					1655:1665	preparation	1655:1665	preparation of GAG-based graft copolymers	1655:1695	This new method for preparation of GAG-based graft copolymers enables a wide range of graft density, and can be used to explore applications of PG mimics as new biomaterials with tunable biochemical and biomechanical functions.
25171516	8	68	with	mimics	838:843	arg1	heparin					850:856	heparin	850:856	heparin	850:856	PG mimics with heparin or chondroitin sulfate side chains and four different ratios of GAG side chain result in graft copolymers with a wide range of sizes.
25171516	8	68	with	mimics	838:843	arg1	sulfate					873:879	chondroitin sulfate	861:879	chondroitin sulfate	861:879	PG mimics with heparin or chondroitin sulfate side chains and four different ratios of GAG side chain result in graft copolymers with a wide range of sizes.
25171516	14	69	theme	GAG-based	1670:1678	arg1	copolymers					1686:1695	GAG-based graft copolymers	1670:1695	GAG-based graft copolymers	1670:1695	This new method for preparation of GAG-based graft copolymers enables a wide range of graft density, and can be used to explore applications of PG mimics as new biomaterials with tunable biochemical and biomechanical functions.
25171516	13	70	theme	lowest	1509:1514	arg1	density					1522:1528	The lowest graft density	1505:1528	The lowest graft density of heparin-containing PG	1505:1553	The lowest graft density of heparin-containing PG mimic also performs well with respect to growth factor delivery from a surface.
25171516	1	71	theme	glycosylated	146:157	arg1	proteins					159:166	important glycosylated proteins	136:166	important glycosylated proteins found on the cell surface and in the extracellular matrix	136:224	Proteoglycans (PGs) are important glycosylated proteins found on the cell surface and in the extracellular matrix.
25171516	1	71	theme	glycosylated	146:157	arg1	Proteoglycans					112:124	Proteoglycans	112:124	Proteoglycans (PGs)	112:130	Proteoglycans (PGs) are important glycosylated proteins found on the cell surface and in the extracellular matrix.
25171516	10	72	theme	light	1202:1206	arg1	scattering					1208:1217	dynamic light scattering	1194:1217	dynamic light scattering	1194:1217	Effective hydrodynamic diameter and zeta potential are determined using dynamic light scattering and electrophoretic mobility measurements.
25171516	0	73	theme	glycosaminoglycan	85:101	arg1	density					103:109	tunable glycosaminoglycan density	77:109	tunable glycosaminoglycan density	77:109	Synthesis and characterization of proteoglycan-mimetic graft copolymers with tunable glycosaminoglycan density.
25171516	10	74	theme	electrophoretic	1223:1237	arg1	mobility					1239:1246	electrophoretic mobility	1223:1246	electrophoretic mobility	1223:1246	Effective hydrodynamic diameter and zeta potential are determined using dynamic light scattering and electrophoretic mobility measurements.
25171516	2	75	theme	core	249:252	arg1	protein					254:260	a core protein	247:260	a core protein with glycosaminoglycan (GAG) side chains	247:301	They are made up of a core protein with glycosaminoglycan (GAG) side chains.
25171516	14	76	theme	tunable	1814:1820	arg1	functions					1852:1860	tunable biochemical and biomechanical functions	1814:1860	tunable biochemical and biomechanical functions	1814:1860	This new method for preparation of GAG-based graft copolymers enables a wide range of graft density, and can be used to explore applications of PG mimics as new biomaterials with tunable biochemical and biomechanical functions.
25171516	4	77	theme	graft	475:479	arg1	copolymers					481:490	proteoglycan-mimetic graft copolymers	454:490	proteoglycan-mimetic graft copolymers (or neoproteoglycans)	454:512	Here we present a method for the synthesis of proteoglycan-mimetic graft copolymers (or neoproteoglycans) with tunable GAG side-chain composition.
25171516	4	77	theme	graft	475:479	arg1	neoproteoglycans					496:511	neoproteoglycans	496:511	neoproteoglycans	496:511	Here we present a method for the synthesis of proteoglycan-mimetic graft copolymers (or neoproteoglycans) with tunable GAG side-chain composition.
25171516	13	78	from	surface	1626:1632	arg1	delivery					1610:1617	growth factor delivery	1596:1617	growth factor delivery from a surface	1596:1632	The lowest graft density of heparin-containing PG mimic also performs well with respect to growth factor delivery from a surface.
25171516	9	79	theme	attentuated	1027:1037	arg1	Fourier					1057:1063	attentuated total reflectance Fourier	1027:1063	attentuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) and (1)H NMR	1027:1119	The chemistry is confirmed through attentuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) and (1)H NMR.
25171516	3	80	theme	chains	353:358	arg1	number					334:339	number	334:339	number	334:339	Variations in composition and number of GAG side chains lead to a vast array of PG sizes and functions.
25171516	3	80	theme	chains	353:358	arg1	composition					318:328	composition	318:328	composition	318:328	Variations in composition and number of GAG side chains lead to a vast array of PG sizes and functions.
25171516	9	81	theme	reflectance	1045:1055	arg1	Fourier					1057:1063	attentuated total reflectance Fourier	1027:1063	attentuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) and (1)H NMR	1027:1119	The chemistry is confirmed through attentuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) and (1)H NMR.
25171516	1	82	located	found	168:172	arg1	matrix					219:224	the extracellular matrix	201:224	the extracellular matrix	201:224	Proteoglycans (PGs) are important glycosylated proteins found on the cell surface and in the extracellular matrix.
25171516	1	82	located	found	168:172	arg1	surface					186:192	the cell surface	177:192	the cell surface	177:192	Proteoglycans (PGs) are important glycosylated proteins found on the cell surface and in the extracellular matrix.
25171516	1	82	located	found	168:172	arg2	proteins					159:166	important glycosylated proteins	136:166	important glycosylated proteins found on the cell surface and in the extracellular matrix	136:224	Proteoglycans (PGs) are important glycosylated proteins found on the cell surface and in the extracellular matrix.
25171516	1	82	located	found	168:172	arg2	Proteoglycans					112:124	Proteoglycans	112:124	Proteoglycans (PGs)	112:130	Proteoglycans (PGs) are important glycosylated proteins found on the cell surface and in the extracellular matrix.
25171516	3	83	theme	GAG	344:346	arg1	chains					353:358	GAG side chains	344:358	GAG side chains	344:358	Variations in composition and number of GAG side chains lead to a vast array of PG sizes and functions.
25171516	8	84	theme	chondroitin	861:871	arg1	sulfate					873:879	chondroitin sulfate	861:879	chondroitin sulfate	861:879	PG mimics with heparin or chondroitin sulfate side chains and four different ratios of GAG side chain result in graft copolymers with a wide range of sizes.
25171516	3	85	theme	vast	370:373	arg1	array					375:379	a vast array	368:379	a vast array of PG sizes and functions	368:405	Variations in composition and number of GAG side chains lead to a vast array of PG sizes and functions.
25171516	4	86	theme	GAG	527:529	arg1	composition					542:552	tunable GAG side-chain composition	519:552	tunable GAG side-chain composition	519:552	Here we present a method for the synthesis of proteoglycan-mimetic graft copolymers (or neoproteoglycans) with tunable GAG side-chain composition.
25171516	3	87	theme	functions	397:405	arg1	array					375:379	a vast array	368:379	a vast array of PG sizes and functions	368:405	Variations in composition and number of GAG side chains lead to a vast array of PG sizes and functions.
25171516	7	88	theme	hyaluronan	786:795	arg1	backbone					797:804	the hyaluronan backbone	782:804	the hyaluronan backbone through reductive amination	782:832	Either chondroitin sulfate or heparin is grafted by the reducing end on to the hyaluronan backbone through reductive amination.
25171516	8	89	theme	side	881:884	arg1	chains					886:891	side chains	881:891	side chains	881:891	PG mimics with heparin or chondroitin sulfate side chains and four different ratios of GAG side chain result in graft copolymers with a wide range of sizes.
25171516	0	90	theme	graft	55:59	arg1	copolymers					61:70	proteoglycan-mimetic graft copolymers	34:70	proteoglycan-mimetic graft copolymers	34:70	Synthesis and characterization of proteoglycan-mimetic graft copolymers with tunable glycosaminoglycan density.
25171516	9	91	dep	NMR	1117:1119	arg1	1					1113:1113	1	1113:1113	1	1113:1113	The chemistry is confirmed through attentuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) and (1)H NMR.
25171516	3	92	theme	sizes	387:391	arg1	array					375:379	a vast array	368:379	a vast array of PG sizes and functions	368:405	Variations in composition and number of GAG side chains lead to a vast array of PG sizes and functions.
25171516	8	93	theme	different	902:910	arg1	ratios					912:917	four different ratios	897:917	four different ratios of GAG side chain	897:935	PG mimics with heparin or chondroitin sulfate side chains and four different ratios of GAG side chain result in graft copolymers with a wide range of sizes.
25171516	9	94	dep	Fourier	1057:1063	arg1	transform					1065:1073	transform	1065:1073	transform infrared spectroscopy (ATR-FTIR) and (1)H NMR	1065:1119	The chemistry is confirmed through attentuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) and (1)H NMR.
25171516	5	95	dep	GAGs	590:593	arg1	GAGs					590:593	three different GAGs	574:593	three different GAGs: hyaluronan, chondroitin sulfate, and heparin	574:639	This is done using three different GAGs: hyaluronan, chondroitin sulfate, and heparin.
25171516	5	95	dep	GAGs	590:593	arg1	hyaluronan					596:605	hyaluronan	596:605	hyaluronan	596:605	This is done using three different GAGs: hyaluronan, chondroitin sulfate, and heparin.
25171516	5	95	dep	GAGs	590:593	arg1	sulfate					620:626	chondroitin sulfate	608:626	chondroitin sulfate	608:626	This is done using three different GAGs: hyaluronan, chondroitin sulfate, and heparin.
25171516	5	95	dep	GAGs	590:593	arg1	heparin					633:639	heparin	633:639	heparin	633:639	This is done using three different GAGs: hyaluronan, chondroitin sulfate, and heparin.
25171516	9	96	theme	H	1115:1115	arg1	NMR					1117:1119	(1)H NMR	1112:1119	(1)H NMR	1112:1119	The chemistry is confirmed through attentuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) and (1)H NMR.
25171516	8	97	theme	side	926:929	arg1	chain					931:935	GAG side chain	922:935	GAG side chain	922:935	PG mimics with heparin or chondroitin sulfate side chains and four different ratios of GAG side chain result in graft copolymers with a wide range of sizes.
25171516	11	98	theme	mesenchymal	1371:1381	arg1	cells					1388:1392	mesenchymal stem cells	1371:1392	mesenchymal stem cells (MSCs)	1371:1399	Graft copolymers were tested for their ability to bind and deliver basic fibroblast growth factor (FGF-2) to mesenchymal stem cells (MSCs).
25171516	11	98	theme	mesenchymal	1371:1381	arg1	MSCs					1395:1398	MSCs	1395:1398	MSCs	1395:1398	Graft copolymers were tested for their ability to bind and deliver basic fibroblast growth factor (FGF-2) to mesenchymal stem cells (MSCs).
25171516	3	99	from	Variations	304:313	arg1	number					334:339	number	334:339	number	334:339	Variations in composition and number of GAG side chains lead to a vast array of PG sizes and functions.
25171516	3	99	from	Variations	304:313	arg1	composition					318:328	composition	318:328	composition	318:328	Variations in composition and number of GAG side chains lead to a vast array of PG sizes and functions.
25171516	4	100	with	synthesis	441:449	arg1	composition					542:552	tunable GAG side-chain composition	519:552	tunable GAG side-chain composition	519:552	Here we present a method for the synthesis of proteoglycan-mimetic graft copolymers (or neoproteoglycans) with tunable GAG side-chain composition.
25171516	10	101	theme	hydrodynamic	1132:1143	arg1	diameter					1145:1152	Effective hydrodynamic diameter	1122:1152	Effective hydrodynamic diameter	1122:1152	Effective hydrodynamic diameter and zeta potential are determined using dynamic light scattering and electrophoretic mobility measurements.
28245197	2	0	from	gradients	411:419	arg1	strength					435:442	strength	435:442	strength	435:442	The pericarp showed excellent impact characteristics, and mechanical tests of its individual components revealed gradients in stiffness, strength and elongation at break from the outer to the inner layer of the pericarp.
28245197	2	0	from	gradients	411:419	arg1	stiffness					424:432	stiffness	424:432	stiffness	424:432	The pericarp showed excellent impact characteristics, and mechanical tests of its individual components revealed gradients in stiffness, strength and elongation at break from the outer to the inner layer of the pericarp.
28245197	2	0	from	gradients	411:419	arg1	break					462:466	break	462:466	break from the outer to the inner layer of the pericarp	462:516	The pericarp showed excellent impact characteristics, and mechanical tests of its individual components revealed gradients in stiffness, strength and elongation at break from the outer to the inner layer of the pericarp.
28245197	2	0	from	gradients	411:419	arg1	elongation					448:457	elongation	448:457	elongation	448:457	The pericarp showed excellent impact characteristics, and mechanical tests of its individual components revealed gradients in stiffness, strength and elongation at break from the outer to the inner layer of the pericarp.
28245197	8	1	theme	types	1612:1616	arg1	properties					1582:1591	desirable properties	1572:1591	desirable properties of different fibre types	1572:1616	In combination, our results show that an asymmetric, systematic variation of fibre properties can successfully combine desirable properties of different fibre types, suggesting new routes for the development of high-performance composites, and improving our understanding of the structure-function relationship of the coconut pericarp.
28245197	0	2	with	fibre-composites	77:92	arg1	properties					160:169	preserved flexural and tensile properties	129:169	preserved flexural and tensile properties	129:169	Functional gradients in the pericarp of the green coconut inspire asymmetric fibre-composites with improved impact strength, and preserved flexural and tensile properties.
28245197	0	2	with	fibre-composites	77:92	arg1	strength					115:122	improved impact strength	99:122	improved impact strength	99:122	Functional gradients in the pericarp of the green coconut inspire asymmetric fibre-composites with improved impact strength, and preserved flexural and tensile properties.
28245197	8	3	theme	composites	1681:1690	arg1	development					1649:1659	the development	1645:1659	the development of high-performance composites	1645:1690	In combination, our results show that an asymmetric, systematic variation of fibre properties can successfully combine desirable properties of different fibre types, suggesting new routes for the development of high-performance composites, and improving our understanding of the structure-function relationship of the coconut pericarp.
28245197	1	4	theme	coconut	269:275	arg1	pericarp					247:254	the pericarp	243:254	the pericarp of the green coconut (Cocos nucifera L.)	243:295	Here we investigate the mechanical properties and structural design of the pericarp of the green coconut (Cocos nucifera L.).
28245197	4	5	theme	graded	811:816	arg1	composites					818:827	the graded composites	807:827	the graded composites	807:827	Tensile properties of the graded composites were largely determined by the 'weakest' fibre, irrespective of the fibre distribution.
28245197	7	6	theme	fibre	1409:1413	arg1	fractions					1415:1423	identical fibre fractions	1399:1423	identical fibre fractions	1399:1423	The impact strength of the graded composites showed a similar orientation-dependence, and peak values exceeded the impact strength of a non-graded reference composite containing identical fibre fractions by up to a factor of three.
28245197	3	7	theme	fibre-reinforced	661:676	arg1	polylactide					678:688	fibre-reinforced polylactide	661:688	simple, graded, cellulose fibre-reinforced polylactide (PLA) composites	635:705	In order to understand more about the potential effect of such gradients on 'bulk' material properties, we designed simple, graded, cellulose fibre-reinforced polylactide (PLA) composites by stacking layers reinforced with fibres of different mechanical properties.
28245197	3	7	theme	fibre-reinforced	661:676	arg1	PLA					691:693	PLA	691:693	PLA	691:693	In order to understand more about the potential effect of such gradients on 'bulk' material properties, we designed simple, graded, cellulose fibre-reinforced polylactide (PLA) composites by stacking layers reinforced with fibres of different mechanical properties.
28245197	6	8	theme	strongest	1114:1122	arg1	samples					1134:1140	the strongest reference samples	1110:1140	the strongest reference samples	1110:1140	Bio-inspired, asymmetrically graded composites showed a flexural strength and modulus comparable to that of the strongest reference samples, but the elongation at maximum load was dependent on the specimen orientation.
28245197	8	9	theme	structure-function	1732:1749	arg1	relationship					1751:1762	the structure-function relationship	1728:1762	the structure-function relationship of the coconut pericarp	1728:1786	In combination, our results show that an asymmetric, systematic variation of fibre properties can successfully combine desirable properties of different fibre types, suggesting new routes for the development of high-performance composites, and improving our understanding of the structure-function relationship of the coconut pericarp.
28245197	6	10	theme	specimen	1199:1206	arg1	orientation					1208:1218	the specimen orientation	1195:1218	the specimen orientation	1195:1218	Bio-inspired, asymmetrically graded composites showed a flexural strength and modulus comparable to that of the strongest reference samples, but the elongation at maximum load was dependent on the specimen orientation.
28245197	0	11	theme	preserved	129:137	arg1	properties					160:169	preserved flexural and tensile properties	129:169	preserved flexural and tensile properties	129:169	Functional gradients in the pericarp of the green coconut inspire asymmetric fibre-composites with improved impact strength, and preserved flexural and tensile properties.
28245197	1	12	theme	structural	222:231	arg1	design					233:238	structural design	222:238	structural design	222:238	Here we investigate the mechanical properties and structural design of the pericarp of the green coconut (Cocos nucifera L.).
28245197	3	13	theme	potential	557:565	arg1	effect					567:572	the potential effect	553:572	the potential effect of such gradients on 'bulk' material properties	553:620	In order to understand more about the potential effect of such gradients on 'bulk' material properties, we designed simple, graded, cellulose fibre-reinforced polylactide (PLA) composites by stacking layers reinforced with fibres of different mechanical properties.
28245197	8	14	theme	coconut	1771:1777	arg1	pericarp					1779:1786	the coconut pericarp	1767:1786	the coconut pericarp	1767:1786	In combination, our results show that an asymmetric, systematic variation of fibre properties can successfully combine desirable properties of different fibre types, suggesting new routes for the development of high-performance composites, and improving our understanding of the structure-function relationship of the coconut pericarp.
28245197	3	15	theme	mechanical	762:771	arg1	properties					773:782	different mechanical properties	752:782	different mechanical properties	752:782	In order to understand more about the potential effect of such gradients on 'bulk' material properties, we designed simple, graded, cellulose fibre-reinforced polylactide (PLA) composites by stacking layers reinforced with fibres of different mechanical properties.
28245197	6	16	theme	maximum	1165:1171	arg1	load					1173:1176	maximum load	1165:1176	maximum load	1165:1176	Bio-inspired, asymmetrically graded composites showed a flexural strength and modulus comparable to that of the strongest reference samples, but the elongation at maximum load was dependent on the specimen orientation.
28245197	0	17	theme	coconut	50:56	arg1	pericarp					28:35	the pericarp	24:35	the pericarp of the green coconut	24:56	Functional gradients in the pericarp of the green coconut inspire asymmetric fibre-composites with improved impact strength, and preserved flexural and tensile properties.
28245197	3	18	theme	gradients	582:590	arg1	effect					567:572	the potential effect	553:572	the potential effect of such gradients on 'bulk' material properties	553:620	In order to understand more about the potential effect of such gradients on 'bulk' material properties, we designed simple, graded, cellulose fibre-reinforced polylactide (PLA) composites by stacking layers reinforced with fibres of different mechanical properties.
28245197	3	19	dep	understand	531:540	arg1	to					528:529	to	528:529	to	528:529	In order to understand more about the potential effect of such gradients on 'bulk' material properties, we designed simple, graded, cellulose fibre-reinforced polylactide (PLA) composites by stacking layers reinforced with fibres of different mechanical properties.
28245197	0	20	theme	asymmetric	66:75	arg1	fibre-composites					77:92	asymmetric fibre-composites	66:92	asymmetric fibre-composites with improved impact strength, and preserved flexural and tensile properties	66:169	Functional gradients in the pericarp of the green coconut inspire asymmetric fibre-composites with improved impact strength, and preserved flexural and tensile properties.
28245197	4	21	theme	distribution	903:914	arg1	irrespective					877:888	irrespective	877:888	irrespective	877:888	Tensile properties of the graded composites were largely determined by the 'weakest' fibre, irrespective of the fibre distribution.
28245197	6	22	dep	Bio-inspired	1002:1013	arg1	graded					1031:1036	graded	1031:1036	graded	1031:1036	Bio-inspired, asymmetrically graded composites showed a flexural strength and modulus comparable to that of the strongest reference samples, but the elongation at maximum load was dependent on the specimen orientation.
28245197	6	23	from	load	1173:1176	arg1	dependent					1182:1190	dependent	1182:1190	dependent	1182:1190	Bio-inspired, asymmetrically graded composites showed a flexural strength and modulus comparable to that of the strongest reference samples, but the elongation at maximum load was dependent on the specimen orientation.
28245197	6	23	from	load	1173:1176	arg1	elongation					1151:1160	the elongation	1147:1160	the elongation at maximum load	1147:1176	Bio-inspired, asymmetrically graded composites showed a flexural strength and modulus comparable to that of the strongest reference samples, but the elongation at maximum load was dependent on the specimen orientation.
28245197	2	24	theme	individual	380:389	arg1	components					391:400	its individual components	376:400	its individual components	376:400	The pericarp showed excellent impact characteristics, and mechanical tests of its individual components revealed gradients in stiffness, strength and elongation at break from the outer to the inner layer of the pericarp.
28245197	3	25	dep	simple	635:640	arg1	cellulose					651:659	cellulose	651:659	cellulose	651:659	In order to understand more about the potential effect of such gradients on 'bulk' material properties, we designed simple, graded, cellulose fibre-reinforced polylactide (PLA) composites by stacking layers reinforced with fibres of different mechanical properties.
28245197	3	25	dep	simple	635:640	arg1	graded					643:648	graded	643:648	graded	643:648	In order to understand more about the potential effect of such gradients on 'bulk' material properties, we designed simple, graded, cellulose fibre-reinforced polylactide (PLA) composites by stacking layers reinforced with fibres of different mechanical properties.
28245197	7	26	theme	peak	1311:1314	arg1	values					1316:1321	peak values	1311:1321	peak values	1311:1321	The impact strength of the graded composites showed a similar orientation-dependence, and peak values exceeded the impact strength of a non-graded reference composite containing identical fibre fractions by up to a factor of three.
28245197	2	27	from	break	462:466	arg1	strength					435:442	strength	435:442	strength	435:442	The pericarp showed excellent impact characteristics, and mechanical tests of its individual components revealed gradients in stiffness, strength and elongation at break from the outer to the inner layer of the pericarp.
28245197	2	27	from	break	462:466	arg1	stiffness					424:432	stiffness	424:432	stiffness	424:432	The pericarp showed excellent impact characteristics, and mechanical tests of its individual components revealed gradients in stiffness, strength and elongation at break from the outer to the inner layer of the pericarp.
28245197	2	27	from	break	462:466	arg1	gradients					411:419	gradients	411:419	gradients in stiffness, strength and elongation at break from the outer to the inner layer of the pericarp	411:516	The pericarp showed excellent impact characteristics, and mechanical tests of its individual components revealed gradients in stiffness, strength and elongation at break from the outer to the inner layer of the pericarp.
28245197	2	27	from	break	462:466	arg1	elongation					448:457	elongation	448:457	elongation	448:457	The pericarp showed excellent impact characteristics, and mechanical tests of its individual components revealed gradients in stiffness, strength and elongation at break from the outer to the inner layer of the pericarp.
28245197	0	28	theme	Functional	0:9	arg1	gradients					11:19	Functional gradients	0:19	Functional gradients in the pericarp of the green coconut	0:56	Functional gradients in the pericarp of the green coconut inspire asymmetric fibre-composites with improved impact strength, and preserved flexural and tensile properties.
28245197	8	29	theme	pericarp	1779:1786	arg1	relationship					1751:1762	the structure-function relationship	1728:1762	the structure-function relationship of the coconut pericarp	1728:1786	In combination, our results show that an asymmetric, systematic variation of fibre properties can successfully combine desirable properties of different fibre types, suggesting new routes for the development of high-performance composites, and improving our understanding of the structure-function relationship of the coconut pericarp.
28245197	1	30	theme	nucifera	284:291	arg1	L					293:293	Cocos nucifera L.	278:294	Cocos nucifera L.	278:294	Here we investigate the mechanical properties and structural design of the pericarp of the green coconut (Cocos nucifera L.).
28245197	1	30	theme	nucifera	284:291	arg1	coconut					269:275	the green coconut	259:275	the green coconut (Cocos nucifera L.)	259:295	Here we investigate the mechanical properties and structural design of the pericarp of the green coconut (Cocos nucifera L.).
28245197	7	31	theme	similar	1275:1281	arg1	orientation-dependence					1283:1304	a similar orientation-dependence	1273:1304	a similar orientation-dependence	1273:1304	The impact strength of the graded composites showed a similar orientation-dependence, and peak values exceeded the impact strength of a non-graded reference composite containing identical fibre fractions by up to a factor of three.
28245197	6	32	theme	Bio-inspired	1002:1013	arg1	composites					1038:1047	Bio-inspired, asymmetrically graded composites	1002:1047	Bio-inspired, asymmetrically graded composites	1002:1047	Bio-inspired, asymmetrically graded composites showed a flexural strength and modulus comparable to that of the strongest reference samples, but the elongation at maximum load was dependent on the specimen orientation.
28245197	4	33	theme	Tensile	785:791	arg1	properties					793:802	Tensile properties	785:802	Tensile properties of the graded composites	785:827	Tensile properties of the graded composites were largely determined by the 'weakest' fibre, irrespective of the fibre distribution.
28245197	8	34	theme	properties	1536:1545	arg1	variation					1517:1525	an asymmetric, systematic variation	1491:1525	an asymmetric, systematic variation of fibre properties	1491:1545	In combination, our results show that an asymmetric, systematic variation of fibre properties can successfully combine desirable properties of different fibre types, suggesting new routes for the development of high-performance composites, and improving our understanding of the structure-function relationship of the coconut pericarp.
28245197	2	35	theme	impact	328:333	arg1	characteristics					335:349	excellent impact characteristics	318:349	excellent impact characteristics	318:349	The pericarp showed excellent impact characteristics, and mechanical tests of its individual components revealed gradients in stiffness, strength and elongation at break from the outer to the inner layer of the pericarp.
28245197	7	36	theme	graded	1248:1253	arg1	composites					1255:1264	the graded composites	1244:1264	the graded composites	1244:1264	The impact strength of the graded composites showed a similar orientation-dependence, and peak values exceeded the impact strength of a non-graded reference composite containing identical fibre fractions by up to a factor of three.
28245197	3	37	theme	simple	635:640	arg1	composites					696:705	simple, graded, cellulose fibre-reinforced polylactide (PLA) composites	635:705	simple, graded, cellulose fibre-reinforced polylactide (PLA) composites	635:705	In order to understand more about the potential effect of such gradients on 'bulk' material properties, we designed simple, graded, cellulose fibre-reinforced polylactide (PLA) composites by stacking layers reinforced with fibres of different mechanical properties.
28245197	8	38	theme	new	1630:1632	arg1	routes					1634:1639	new routes	1630:1639	new routes for the development of high-performance composites	1630:1690	In combination, our results show that an asymmetric, systematic variation of fibre properties can successfully combine desirable properties of different fibre types, suggesting new routes for the development of high-performance composites, and improving our understanding of the structure-function relationship of the coconut pericarp.
28245197	6	39	theme	comparable	1088:1097	arg1	strength					1067:1074	a flexural strength	1056:1074	a flexural strength	1056:1074	Bio-inspired, asymmetrically graded composites showed a flexural strength and modulus comparable to that of the strongest reference samples, but the elongation at maximum load was dependent on the specimen orientation.
28245197	8	40	theme	desirable	1572:1580	arg1	properties					1582:1591	desirable properties	1572:1591	desirable properties of different fibre types	1572:1616	In combination, our results show that an asymmetric, systematic variation of fibre properties can successfully combine desirable properties of different fibre types, suggesting new routes for the development of high-performance composites, and improving our understanding of the structure-function relationship of the coconut pericarp.
28245197	2	41	theme	components	391:400	arg1	tests					367:371	mechanical tests	356:371	mechanical tests of its individual components	356:400	The pericarp showed excellent impact characteristics, and mechanical tests of its individual components revealed gradients in stiffness, strength and elongation at break from the outer to the inner layer of the pericarp.
28245197	2	42	theme	mechanical	356:365	arg1	tests					367:371	mechanical tests	356:371	mechanical tests of its individual components	356:400	The pericarp showed excellent impact characteristics, and mechanical tests of its individual components revealed gradients in stiffness, strength and elongation at break from the outer to the inner layer of the pericarp.
28245197	5	43	theme	asymmetric	960:969	arg1	bending					971:977	pronounced asymmetric bending	949:977	pronounced asymmetric bending	949:977	However, a graded design led to pronounced asymmetric bending and impact properties.
28245197	7	44	theme	impact	1225:1230	arg1	strength					1232:1239	The impact strength	1221:1239	The impact strength of the graded composites	1221:1264	The impact strength of the graded composites showed a similar orientation-dependence, and peak values exceeded the impact strength of a non-graded reference composite containing identical fibre fractions by up to a factor of three.
28245197	7	45	theme	three	1446:1450	arg1	factor					1436:1441	a factor	1434:1441	a factor of three	1434:1450	The impact strength of the graded composites showed a similar orientation-dependence, and peak values exceeded the impact strength of a non-graded reference composite containing identical fibre fractions by up to a factor of three.
28245197	6	46	theme	flexural	1058:1065	arg1	strength					1067:1074	a flexural strength	1056:1074	a flexural strength	1056:1074	Bio-inspired, asymmetrically graded composites showed a flexural strength and modulus comparable to that of the strongest reference samples, but the elongation at maximum load was dependent on the specimen orientation.
28245197	8	47	theme	fibre	1606:1610	arg1	types					1612:1616	different fibre types	1596:1616	different fibre types	1596:1616	In combination, our results show that an asymmetric, systematic variation of fibre properties can successfully combine desirable properties of different fibre types, suggesting new routes for the development of high-performance composites, and improving our understanding of the structure-function relationship of the coconut pericarp.
28245197	8	48	theme	high-performance	1664:1679	arg1	composites					1681:1690	high-performance composites	1664:1690	high-performance composites	1664:1690	In combination, our results show that an asymmetric, systematic variation of fibre properties can successfully combine desirable properties of different fibre types, suggesting new routes for the development of high-performance composites, and improving our understanding of the structure-function relationship of the coconut pericarp.
28245197	1	49	theme	green	263:267	arg1	L					293:293	Cocos nucifera L.	278:294	Cocos nucifera L.	278:294	Here we investigate the mechanical properties and structural design of the pericarp of the green coconut (Cocos nucifera L.).
28245197	1	49	theme	green	263:267	arg1	coconut					269:275	the green coconut	259:275	the green coconut (Cocos nucifera L.)	259:295	Here we investigate the mechanical properties and structural design of the pericarp of the green coconut (Cocos nucifera L.).
28245197	3	50	theme	polylactide	678:688	arg1	composites					696:705	simple, graded, cellulose fibre-reinforced polylactide (PLA) composites	635:705	simple, graded, cellulose fibre-reinforced polylactide (PLA) composites	635:705	In order to understand more about the potential effect of such gradients on 'bulk' material properties, we designed simple, graded, cellulose fibre-reinforced polylactide (PLA) composites by stacking layers reinforced with fibres of different mechanical properties.
28245197	4	51	theme	composites	818:827	arg1	properties					793:802	Tensile properties	785:802	Tensile properties of the graded composites	785:827	Tensile properties of the graded composites were largely determined by the 'weakest' fibre, irrespective of the fibre distribution.
28245197	6	52	theme	reference	1124:1132	arg1	samples					1134:1140	the strongest reference samples	1110:1140	the strongest reference samples	1110:1140	Bio-inspired, asymmetrically graded composites showed a flexural strength and modulus comparable to that of the strongest reference samples, but the elongation at maximum load was dependent on the specimen orientation.
28245197	3	53	theme	stacking	710:717	arg1	layers					719:724	stacking layers	710:724	stacking layers reinforced with fibres of different mechanical properties	710:782	In order to understand more about the potential effect of such gradients on 'bulk' material properties, we designed simple, graded, cellulose fibre-reinforced polylactide (PLA) composites by stacking layers reinforced with fibres of different mechanical properties.
28245197	1	54	theme	mechanical	196:205	arg1	properties					207:216	the mechanical properties	192:216	the mechanical properties	192:216	Here we investigate the mechanical properties and structural design of the pericarp of the green coconut (Cocos nucifera L.).
28245197	7	55	theme	identical	1399:1407	arg1	fractions					1415:1423	identical fibre fractions	1399:1423	identical fibre fractions	1399:1423	The impact strength of the graded composites showed a similar orientation-dependence, and peak values exceeded the impact strength of a non-graded reference composite containing identical fibre fractions by up to a factor of three.
28245197	2	56	from	outer	477:481	arg1	break					462:466	break	462:466	break from the outer to the inner layer of the pericarp	462:516	The pericarp showed excellent impact characteristics, and mechanical tests of its individual components revealed gradients in stiffness, strength and elongation at break from the outer to the inner layer of the pericarp.
28245197	7	57	theme	composite	1378:1386	arg1	strength					1343:1350	the impact strength	1332:1350	the impact strength of a non-graded reference composite containing identical fibre fractions	1332:1423	The impact strength of the graded composites showed a similar orientation-dependence, and peak values exceeded the impact strength of a non-graded reference composite containing identical fibre fractions by up to a factor of three.
28245197	0	58	theme	flexural	139:146	arg1	properties					160:169	preserved flexural and tensile properties	129:169	preserved flexural and tensile properties	129:169	Functional gradients in the pericarp of the green coconut inspire asymmetric fibre-composites with improved impact strength, and preserved flexural and tensile properties.
28245197	2	59	theme	pericarp	509:516	arg1	layer					496:500	the inner layer	486:500	the inner layer of the pericarp	486:516	The pericarp showed excellent impact characteristics, and mechanical tests of its individual components revealed gradients in stiffness, strength and elongation at break from the outer to the inner layer of the pericarp.
28245197	7	60	theme	non-graded	1357:1366	arg1	composite					1378:1386	a non-graded reference composite	1355:1386	a non-graded reference composite containing identical fibre fractions	1355:1423	The impact strength of the graded composites showed a similar orientation-dependence, and peak values exceeded the impact strength of a non-graded reference composite containing identical fibre fractions by up to a factor of three.
28245197	0	61	theme	tensile	152:158	arg1	properties					160:169	preserved flexural and tensile properties	129:169	preserved flexural and tensile properties	129:169	Functional gradients in the pericarp of the green coconut inspire asymmetric fibre-composites with improved impact strength, and preserved flexural and tensile properties.
28245197	8	62	theme	relationship	1751:1762	arg1	understanding					1711:1723	our understanding	1707:1723	our understanding of the structure-function relationship of the coconut pericarp	1707:1786	In combination, our results show that an asymmetric, systematic variation of fibre properties can successfully combine desirable properties of different fibre types, suggesting new routes for the development of high-performance composites, and improving our understanding of the structure-function relationship of the coconut pericarp.
28245197	7	63	theme	reference	1368:1376	arg1	composite					1378:1386	a non-graded reference composite	1355:1386	a non-graded reference composite containing identical fibre fractions	1355:1423	The impact strength of the graded composites showed a similar orientation-dependence, and peak values exceeded the impact strength of a non-graded reference composite containing identical fibre fractions by up to a factor of three.
28245197	7	64	theme	impact	1336:1341	arg1	strength					1343:1350	the impact strength	1332:1350	the impact strength of a non-graded reference composite containing identical fibre fractions	1332:1423	The impact strength of the graded composites showed a similar orientation-dependence, and peak values exceeded the impact strength of a non-graded reference composite containing identical fibre fractions by up to a factor of three.
28245197	3	65	theme	properties	773:782	arg1	fibres					742:747	fibres	742:747	fibres of different mechanical properties	742:782	In order to understand more about the potential effect of such gradients on 'bulk' material properties, we designed simple, graded, cellulose fibre-reinforced polylactide (PLA) composites by stacking layers reinforced with fibres of different mechanical properties.
28245197	4	66	dep	fibre	870:874	arg1	irrespective					877:888	irrespective	877:888	irrespective	877:888	Tensile properties of the graded composites were largely determined by the 'weakest' fibre, irrespective of the fibre distribution.
28245197	3	67	theme	such	577:580	arg1	gradients					582:590	such gradients	577:590	such gradients	577:590	In order to understand more about the potential effect of such gradients on 'bulk' material properties, we designed simple, graded, cellulose fibre-reinforced polylactide (PLA) composites by stacking layers reinforced with fibres of different mechanical properties.
28245197	8	68	theme	asymmetric	1494:1503	arg1	variation					1517:1525	an asymmetric, systematic variation	1491:1525	an asymmetric, systematic variation of fibre properties	1491:1545	In combination, our results show that an asymmetric, systematic variation of fibre properties can successfully combine desirable properties of different fibre types, suggesting new routes for the development of high-performance composites, and improving our understanding of the structure-function relationship of the coconut pericarp.
28245197	3	69	theme	different	752:760	arg1	properties					773:782	different mechanical properties	752:782	different mechanical properties	752:782	In order to understand more about the potential effect of such gradients on 'bulk' material properties, we designed simple, graded, cellulose fibre-reinforced polylactide (PLA) composites by stacking layers reinforced with fibres of different mechanical properties.
28245197	0	70	from	gradients	11:19	arg1	pericarp					28:35	the pericarp	24:35	the pericarp of the green coconut	24:56	Functional gradients in the pericarp of the green coconut inspire asymmetric fibre-composites with improved impact strength, and preserved flexural and tensile properties.
28245197	0	71	theme	improved	99:106	arg1	strength					115:122	improved impact strength	99:122	improved impact strength	99:122	Functional gradients in the pericarp of the green coconut inspire asymmetric fibre-composites with improved impact strength, and preserved flexural and tensile properties.
28245197	4	72	theme	fibre	897:901	arg1	distribution					903:914	the fibre distribution	893:914	the fibre distribution	893:914	Tensile properties of the graded composites were largely determined by the 'weakest' fibre, irrespective of the fibre distribution.
28245197	5	73	theme	graded	928:933	arg1	design					935:940	a graded design	926:940	a graded design	926:940	However, a graded design led to pronounced asymmetric bending and impact properties.
28245197	2	74	theme	inner	490:494	arg1	layer					496:500	the inner layer	486:500	the inner layer of the pericarp	486:516	The pericarp showed excellent impact characteristics, and mechanical tests of its individual components revealed gradients in stiffness, strength and elongation at break from the outer to the inner layer of the pericarp.
28245197	1	75	theme	Cocos	278:282	arg1	L					293:293	Cocos nucifera L.	278:294	Cocos nucifera L.	278:294	Here we investigate the mechanical properties and structural design of the pericarp of the green coconut (Cocos nucifera L.).
28245197	1	75	theme	Cocos	278:282	arg1	coconut					269:275	the green coconut	259:275	the green coconut (Cocos nucifera L.)	259:295	Here we investigate the mechanical properties and structural design of the pericarp of the green coconut (Cocos nucifera L.).
28245197	8	76	theme	fibre	1530:1534	arg1	properties					1536:1545	fibre properties	1530:1545	fibre properties	1530:1545	In combination, our results show that an asymmetric, systematic variation of fibre properties can successfully combine desirable properties of different fibre types, suggesting new routes for the development of high-performance composites, and improving our understanding of the structure-function relationship of the coconut pericarp.
28245197	3	77	theme	material	602:609	arg1	properties					611:620	'bulk' material properties	595:620	'bulk' material properties	595:620	In order to understand more about the potential effect of such gradients on 'bulk' material properties, we designed simple, graded, cellulose fibre-reinforced polylactide (PLA) composites by stacking layers reinforced with fibres of different mechanical properties.
28245197	3	78	from	effect	567:572	arg1	properties					611:620	'bulk' material properties	595:620	'bulk' material properties	595:620	In order to understand more about the potential effect of such gradients on 'bulk' material properties, we designed simple, graded, cellulose fibre-reinforced polylactide (PLA) composites by stacking layers reinforced with fibres of different mechanical properties.
28245197	7	79	contain	containing	1388:1397	arg2	fractions					1415:1423	identical fibre fractions	1399:1423	identical fibre fractions	1399:1423	The impact strength of the graded composites showed a similar orientation-dependence, and peak values exceeded the impact strength of a non-graded reference composite containing identical fibre fractions by up to a factor of three.
28245197	7	79	contain	containing	1388:1397	arg1	composite					1378:1386	a non-graded reference composite	1355:1386	a non-graded reference composite containing identical fibre fractions	1355:1423	The impact strength of the graded composites showed a similar orientation-dependence, and peak values exceeded the impact strength of a non-graded reference composite containing identical fibre fractions by up to a factor of three.
28245197	7	80	theme	composites	1255:1264	arg1	strength					1232:1239	The impact strength	1221:1239	The impact strength of the graded composites	1221:1264	The impact strength of the graded composites showed a similar orientation-dependence, and peak values exceeded the impact strength of a non-graded reference composite containing identical fibre fractions by up to a factor of three.
28245197	3	81	theme	bulk	596:599	arg1	properties					611:620	'bulk' material properties	595:620	'bulk' material properties	595:620	In order to understand more about the potential effect of such gradients on 'bulk' material properties, we designed simple, graded, cellulose fibre-reinforced polylactide (PLA) composites by stacking layers reinforced with fibres of different mechanical properties.
28245197	0	82	theme	impact	108:113	arg1	strength					115:122	improved impact strength	99:122	improved impact strength	99:122	Functional gradients in the pericarp of the green coconut inspire asymmetric fibre-composites with improved impact strength, and preserved flexural and tensile properties.
28245197	0	83	theme	green	44:48	arg1	coconut					50:56	the green coconut	40:56	the green coconut	40:56	Functional gradients in the pericarp of the green coconut inspire asymmetric fibre-composites with improved impact strength, and preserved flexural and tensile properties.
28245197	5	84	theme	impact	983:988	arg1	properties					990:999	impact properties	983:999	impact properties	983:999	However, a graded design led to pronounced asymmetric bending and impact properties.
28245197	2	85	theme	excellent	318:326	arg1	characteristics					335:349	excellent impact characteristics	318:349	excellent impact characteristics	318:349	The pericarp showed excellent impact characteristics, and mechanical tests of its individual components revealed gradients in stiffness, strength and elongation at break from the outer to the inner layer of the pericarp.
28245197	8	86	dep	asymmetric	1494:1503	arg1	systematic					1506:1515	systematic	1506:1515	systematic	1506:1515	In combination, our results show that an asymmetric, systematic variation of fibre properties can successfully combine desirable properties of different fibre types, suggesting new routes for the development of high-performance composites, and improving our understanding of the structure-function relationship of the coconut pericarp.
28245197	5	87	theme	pronounced	949:958	arg1	bending					971:977	pronounced asymmetric bending	949:977	pronounced asymmetric bending	949:977	However, a graded design led to pronounced asymmetric bending and impact properties.
28245197	8	88	theme	different	1596:1604	arg1	types					1612:1616	different fibre types	1596:1616	different fibre types	1596:1616	In combination, our results show that an asymmetric, systematic variation of fibre properties can successfully combine desirable properties of different fibre types, suggesting new routes for the development of high-performance composites, and improving our understanding of the structure-function relationship of the coconut pericarp.
28245197	1	89	theme	pericarp	247:254	arg1	properties					207:216	the mechanical properties	192:216	the mechanical properties	192:216	Here we investigate the mechanical properties and structural design of the pericarp of the green coconut (Cocos nucifera L.).
28245197	1	89	theme	pericarp	247:254	arg1	design					233:238	structural design	222:238	structural design	222:238	Here we investigate the mechanical properties and structural design of the pericarp of the green coconut (Cocos nucifera L.).
28117730	0	0	theme	Small	79:83	arg1	Scattering					97:106	A Small Angle X-ray Scattering	77:106	A Small Angle X-ray Scattering Study	77:112	Nano-Structural Investigation on Cellulose Highly Dissolved in Ionic Liquid: A Small Angle X-ray Scattering Study.
28117730	1	1	theme	mol	335:337	arg1	%					339:339	0-100 mol %	329:339	0-100 mol %	329:339	We investigated nano-structural changes of cellulose dissolved in 1-ethyl-3-methylimidazolium acetate-an ionic liquid (IL)-using a small angle X-ray scattering (SAXS) technique over the entire concentration range (0-100 mol %).
28117730	1	1	theme	mol	335:337	arg1	range					322:326	the entire concentration range	297:326	the entire concentration range (0-100 mol %)	297:340	We investigated nano-structural changes of cellulose dissolved in 1-ethyl-3-methylimidazolium acetate-an ionic liquid (IL)-using a small angle X-ray scattering (SAXS) technique over the entire concentration range (0-100 mol %).
28117730	4	2	theme	nano-structures	824:838	arg1	emergence					796:804	the emergence	792:804	the emergence of two aggregated nano-structures in the concentration range of 30-80 mol %	792:880	Furthermore, we observed the emergence of two aggregated nano-structures in the concentration range of 30-80 mol %.
28117730	1	3	theme	cellulose	158:166	arg1	changes					147:153	nano-structural changes	131:153	nano-structural changes of cellulose dissolved in 1-ethyl-3-methylimidazolium acetate-an ionic liquid (IL)-using a small angle X-ray scattering (SAXS) technique	131:290	We investigated nano-structural changes of cellulose dissolved in 1-ethyl-3-methylimidazolium acetate-an ionic liquid (IL)-using a small angle X-ray scattering (SAXS) technique over the entire concentration range (0-100 mol %).
28117730	1	4	theme	X-ray	258:262	arg1	scattering					264:273	a small angle X-ray scattering	244:273	1-ethyl-3-methylimidazolium acetate-an ionic liquid (IL)-using a small angle X-ray scattering (SAXS) technique	181:290	We investigated nano-structural changes of cellulose dissolved in 1-ethyl-3-methylimidazolium acetate-an ionic liquid (IL)-using a small angle X-ray scattering (SAXS) technique over the entire concentration range (0-100 mol %).
28117730	1	4	theme	X-ray	258:262	arg1	SAXS					276:279	SAXS	276:279	SAXS	276:279	We investigated nano-structural changes of cellulose dissolved in 1-ethyl-3-methylimidazolium acetate-an ionic liquid (IL)-using a small angle X-ray scattering (SAXS) technique over the entire concentration range (0-100 mol %).
28117730	3	5	theme	multiple	660:667	arg1	groups					672:677	multiple OH groups	660:677	multiple OH groups of different cellulose molecules	660:710	This behavior is explained by the presence of the anion bridging, whereby an anion prefers to interact with multiple OH groups of different cellulose molecules at high concentrations, discovered in our recent work.
28117730	3	5	theme	multiple	660:667	arg1	molecules					702:710	different cellulose molecules	682:710	different cellulose molecules	682:710	This behavior is explained by the presence of the anion bridging, whereby an anion prefers to interact with multiple OH groups of different cellulose molecules at high concentrations, discovered in our recent work.
28117730	0	6	from	Investigation	16:28	arg1	Cellulose					33:41	Cellulose	33:41	Cellulose	33:41	Nano-Structural Investigation on Cellulose Highly Dissolved in Ionic Liquid: A Small Angle X-ray Scattering Study.
28117730	4	7	theme	aggregated	813:822	arg1	nano-structures					824:838	two aggregated nano-structures	809:838	two aggregated nano-structures	809:838	Furthermore, we observed the emergence of two aggregated nano-structures in the concentration range of 30-80 mol %.
28117730	2	8	theme	maximum	467:473	arg1	concentration					475:487	the maximum concentration	463:487	the maximum concentration of dissolution (24-28 mol %)	463:516	Fibril structures of cellulose disappeared at 40 mol % of cellulose, which is a significantly higher concentration than the maximum concentration of dissolution (24-28 mol %) previously determined in this IL.
28117730	8	9	theme	novel	1312:1316	arg1	composite					1331:1339	a novel cellulose/IL composite	1310:1339	a novel cellulose/IL composite	1310:1339	Finally, we unexpectedly proposed a novel cellulose/IL composite: the cellulose/IL mixtures of 30-50 mol % that possess liquid crystallinity are sufficiently hard to be moldable.
28117730	1	10	theme	scattering	264:273	arg1	technique					282:290	1-ethyl-3-methylimidazolium acetate-an ionic liquid (IL)-using a small angle X-ray scattering (SAXS) technique	181:290	1-ethyl-3-methylimidazolium acetate-an ionic liquid (IL)-using a small angle X-ray scattering (SAXS) technique	181:290	We investigated nano-structural changes of cellulose dissolved in 1-ethyl-3-methylimidazolium acetate-an ionic liquid (IL)-using a small angle X-ray scattering (SAXS) technique over the entire concentration range (0-100 mol %).
28117730	0	11	theme	X-ray	91:95	arg1	Scattering					97:106	A Small Angle X-ray Scattering	77:106	A Small Angle X-ray Scattering Study	77:112	Nano-Structural Investigation on Cellulose Highly Dissolved in Ionic Liquid: A Small Angle X-ray Scattering Study.
28117730	0	12	dep	Study	108:112	arg1	Investigation					16:28	Nano-Structural Investigation	0:28	Nano-Structural Investigation on Cellulose Highly Dissolved in Ionic Liquid	0:74	Nano-Structural Investigation on Cellulose Highly Dissolved in Ionic Liquid: A Small Angle X-ray Scattering Study.
28117730	0	13	theme	Angle	85:89	arg1	Scattering					97:106	A Small Angle X-ray Scattering	77:106	A Small Angle X-ray Scattering Study	77:112	Nano-Structural Investigation on Cellulose Highly Dissolved in Ionic Liquid: A Small Angle X-ray Scattering Study.
28117730	6	14	theme	microfibril	1121:1131	arg1	reminiscent					1094:1104	reminiscent	1094:1104	reminiscent	1094:1104	In contrast, the other with 4.1 nm diameter exhibited concentration independence and is reminiscent of a cellulose microfibril, reflecting the occurrence of nanofibrillation.
28117730	6	14	theme	microfibril	1121:1131	arg1	other					1023:1027	other	1023:1027	other	1023:1027	In contrast, the other with 4.1 nm diameter exhibited concentration independence and is reminiscent of a cellulose microfibril, reflecting the occurrence of nanofibrillation.
28117730	2	15	theme	Fibril	343:348	arg1	structures					350:359	Fibril structures	343:359	Fibril structures of cellulose	343:372	Fibril structures of cellulose disappeared at 40 mol % of cellulose, which is a significantly higher concentration than the maximum concentration of dissolution (24-28 mol %) previously determined in this IL.
28117730	3	16	theme	different	682:690	arg1	molecules					702:710	different cellulose molecules	682:710	different cellulose molecules	682:710	This behavior is explained by the presence of the anion bridging, whereby an anion prefers to interact with multiple OH groups of different cellulose molecules at high concentrations, discovered in our recent work.
28117730	7	17	from	understanding	1212:1224	arg1	ILs					1271:1273	ILs	1271:1273	ILs	1271:1273	These results contribute to an understanding of the dissolution mechanism of cellulose in ILs.
28117730	6	18	theme	cellulose	1111:1119	arg1	microfibril					1121:1131	a cellulose microfibril	1109:1131	a cellulose microfibril	1109:1131	In contrast, the other with 4.1 nm diameter exhibited concentration independence and is reminiscent of a cellulose microfibril, reflecting the occurrence of nanofibrillation.
28117730	0	19	theme	Scattering	97:106	arg1	Study					108:112	A Small Angle X-ray Scattering Study	77:112	A Small Angle X-ray Scattering Study	77:112	Nano-Structural Investigation on Cellulose Highly Dissolved in Ionic Liquid: A Small Angle X-ray Scattering Study.
28117730	3	20	theme	OH	669:670	arg1	groups					672:677	multiple OH groups	660:677	multiple OH groups of different cellulose molecules	660:710	This behavior is explained by the presence of the anion bridging, whereby an anion prefers to interact with multiple OH groups of different cellulose molecules at high concentrations, discovered in our recent work.
28117730	3	20	theme	OH	669:670	arg1	molecules					702:710	different cellulose molecules	682:710	different cellulose molecules	682:710	This behavior is explained by the presence of the anion bridging, whereby an anion prefers to interact with multiple OH groups of different cellulose molecules at high concentrations, discovered in our recent work.
28117730	8	21	contain	possess	1388:1394	arg1	mixtures					1359:1366	the cellulose/IL mixtures	1342:1366	the cellulose/IL mixtures of 30-50 mol % that possess liquid crystallinity	1342:1415	Finally, we unexpectedly proposed a novel cellulose/IL composite: the cellulose/IL mixtures of 30-50 mol % that possess liquid crystallinity are sufficiently hard to be moldable.
28117730	8	21	contain	possess	1388:1394	arg2	crystallinity					1403:1415	liquid crystallinity	1396:1415	liquid crystallinity	1396:1415	Finally, we unexpectedly proposed a novel cellulose/IL composite: the cellulose/IL mixtures of 30-50 mol % that possess liquid crystallinity are sufficiently hard to be moldable.
28117730	8	21	contain	possess	1388:1394	arg1	moldable					1445:1452	moldable	1445:1452	moldable	1445:1452	Finally, we unexpectedly proposed a novel cellulose/IL composite: the cellulose/IL mixtures of 30-50 mol % that possess liquid crystallinity are sufficiently hard to be moldable.
28117730	1	22	theme	1-ethyl-3-methylimidazolium	181:207	arg1	technique					282:290	1-ethyl-3-methylimidazolium acetate-an ionic liquid (IL)-using a small angle X-ray scattering (SAXS) technique	181:290	1-ethyl-3-methylimidazolium acetate-an ionic liquid (IL)-using a small angle X-ray scattering (SAXS) technique	181:290	We investigated nano-structural changes of cellulose dissolved in 1-ethyl-3-methylimidazolium acetate-an ionic liquid (IL)-using a small angle X-ray scattering (SAXS) technique over the entire concentration range (0-100 mol %).
28117730	6	23	with	other	1023:1027	arg1	diameter					1041:1048	4.1 nm diameter	1034:1048	4.1 nm diameter	1034:1048	In contrast, the other with 4.1 nm diameter exhibited concentration independence and is reminiscent of a cellulose microfibril, reflecting the occurrence of nanofibrillation.
28117730	1	24	theme	acetate-an	209:218	arg1	technique					282:290	1-ethyl-3-methylimidazolium acetate-an ionic liquid (IL)-using a small angle X-ray scattering (SAXS) technique	181:290	1-ethyl-3-methylimidazolium acetate-an ionic liquid (IL)-using a small angle X-ray scattering (SAXS) technique	181:290	We investigated nano-structural changes of cellulose dissolved in 1-ethyl-3-methylimidazolium acetate-an ionic liquid (IL)-using a small angle X-ray scattering (SAXS) technique over the entire concentration range (0-100 mol %).
28117730	0	25	theme	Nano-Structural	0:14	arg1	Investigation					16:28	Nano-Structural Investigation	0:28	Nano-Structural Investigation on Cellulose Highly Dissolved in Ionic Liquid	0:74	Nano-Structural Investigation on Cellulose Highly Dissolved in Ionic Liquid: A Small Angle X-ray Scattering Study.
28117730	2	26	theme	mol	511:513	arg1	%					515:515	24-28 mol %	505:515	24-28 mol %	505:515	Fibril structures of cellulose disappeared at 40 mol % of cellulose, which is a significantly higher concentration than the maximum concentration of dissolution (24-28 mol %) previously determined in this IL.
28117730	2	26	theme	mol	511:513	arg1	dissolution					492:502	dissolution	492:502	dissolution (24-28 mol %)	492:516	Fibril structures of cellulose disappeared at 40 mol % of cellulose, which is a significantly higher concentration than the maximum concentration of dissolution (24-28 mol %) previously determined in this IL.
28117730	8	27	theme	cellulose/IL	1318:1329	arg1	composite					1331:1339	a novel cellulose/IL composite	1310:1339	a novel cellulose/IL composite	1310:1339	Finally, we unexpectedly proposed a novel cellulose/IL composite: the cellulose/IL mixtures of 30-50 mol % that possess liquid crystallinity are sufficiently hard to be moldable.
28117730	1	28	theme	ionic	220:224	arg1	technique					282:290	1-ethyl-3-methylimidazolium acetate-an ionic liquid (IL)-using a small angle X-ray scattering (SAXS) technique	181:290	1-ethyl-3-methylimidazolium acetate-an ionic liquid (IL)-using a small angle X-ray scattering (SAXS) technique	181:290	We investigated nano-structural changes of cellulose dissolved in 1-ethyl-3-methylimidazolium acetate-an ionic liquid (IL)-using a small angle X-ray scattering (SAXS) technique over the entire concentration range (0-100 mol %).
28117730	3	29	theme	high	715:718	arg1	concentrations					720:733	high concentrations	715:733	high concentrations	715:733	This behavior is explained by the presence of the anion bridging, whereby an anion prefers to interact with multiple OH groups of different cellulose molecules at high concentrations, discovered in our recent work.
28117730	4	30	theme	%	880:880	arg1	range					861:865	the concentration range	843:865	the concentration range of 30-80 mol %	843:880	Furthermore, we observed the emergence of two aggregated nano-structures in the concentration range of 30-80 mol %.
28117730	7	31	from	mechanism	1245:1253	arg1	ILs					1271:1273	ILs	1271:1273	ILs	1271:1273	These results contribute to an understanding of the dissolution mechanism of cellulose in ILs.
28117730	1	32	theme	liquid	226:231	arg1	technique					282:290	1-ethyl-3-methylimidazolium acetate-an ionic liquid (IL)-using a small angle X-ray scattering (SAXS) technique	181:290	1-ethyl-3-methylimidazolium acetate-an ionic liquid (IL)-using a small angle X-ray scattering (SAXS) technique	181:290	We investigated nano-structural changes of cellulose dissolved in 1-ethyl-3-methylimidazolium acetate-an ionic liquid (IL)-using a small angle X-ray scattering (SAXS) technique over the entire concentration range (0-100 mol %).
28117730	3	33	theme	cellulose	692:700	arg1	molecules					702:710	different cellulose molecules	682:710	different cellulose molecules	682:710	This behavior is explained by the presence of the anion bridging, whereby an anion prefers to interact with multiple OH groups of different cellulose molecules at high concentrations, discovered in our recent work.
28117730	4	34	theme	mol	876:878	arg1	%					880:880	30-80 mol %	870:880	30-80 mol %	870:880	Furthermore, we observed the emergence of two aggregated nano-structures in the concentration range of 30-80 mol %.
28117730	3	35	theme	bridging	608:615	arg1	anion					602:606	the anion bridging	598:615	the anion bridging	598:615	This behavior is explained by the presence of the anion bridging, whereby an anion prefers to interact with multiple OH groups of different cellulose molecules at high concentrations, discovered in our recent work.
28117730	5	36	theme	structure	903:911	arg1	diameter					887:894	The diameter	883:894	The diameter of one structure	883:911	The diameter of one structure was 12-20 nm, dependent on concentration, which is ascribed to cellulose chain entanglement.
28117730	5	36	theme	structure	903:911	arg1	nm					923:924	12-20 nm	917:924	12-20 nm	917:924	The diameter of one structure was 12-20 nm, dependent on concentration, which is ascribed to cellulose chain entanglement.
28117730	3	37	theme	molecules	702:710	arg1	groups					672:677	multiple OH groups	660:677	multiple OH groups of different cellulose molecules	660:710	This behavior is explained by the presence of the anion bridging, whereby an anion prefers to interact with multiple OH groups of different cellulose molecules at high concentrations, discovered in our recent work.
28117730	3	37	theme	molecules	702:710	arg1	molecules					702:710	different cellulose molecules	682:710	different cellulose molecules	682:710	This behavior is explained by the presence of the anion bridging, whereby an anion prefers to interact with multiple OH groups of different cellulose molecules at high concentrations, discovered in our recent work.
28117730	8	38	theme	liquid	1396:1401	arg1	crystallinity					1403:1415	liquid crystallinity	1396:1415	liquid crystallinity	1396:1415	Finally, we unexpectedly proposed a novel cellulose/IL composite: the cellulose/IL mixtures of 30-50 mol % that possess liquid crystallinity are sufficiently hard to be moldable.
28117730	2	39	theme	cellulose	364:372	arg1	structures					350:359	Fibril structures	343:359	Fibril structures of cellulose	343:372	Fibril structures of cellulose disappeared at 40 mol % of cellulose, which is a significantly higher concentration than the maximum concentration of dissolution (24-28 mol %) previously determined in this IL.
28117730	1	40	theme	IL	234:235	arg1	technique					282:290	1-ethyl-3-methylimidazolium acetate-an ionic liquid (IL)-using a small angle X-ray scattering (SAXS) technique	181:290	1-ethyl-3-methylimidazolium acetate-an ionic liquid (IL)-using a small angle X-ray scattering (SAXS) technique	181:290	We investigated nano-structural changes of cellulose dissolved in 1-ethyl-3-methylimidazolium acetate-an ionic liquid (IL)-using a small angle X-ray scattering (SAXS) technique over the entire concentration range (0-100 mol %).
28117730	1	41	theme	entire	301:306	arg1	%					339:339	0-100 mol %	329:339	0-100 mol %	329:339	We investigated nano-structural changes of cellulose dissolved in 1-ethyl-3-methylimidazolium acetate-an ionic liquid (IL)-using a small angle X-ray scattering (SAXS) technique over the entire concentration range (0-100 mol %).
28117730	1	41	theme	entire	301:306	arg1	range					322:326	the entire concentration range	297:326	the entire concentration range (0-100 mol %)	297:340	We investigated nano-structural changes of cellulose dissolved in 1-ethyl-3-methylimidazolium acetate-an ionic liquid (IL)-using a small angle X-ray scattering (SAXS) technique over the entire concentration range (0-100 mol %).
28117730	8	42	theme	cellulose/IL	1346:1357	arg1	mixtures					1359:1366	the cellulose/IL mixtures	1342:1366	the cellulose/IL mixtures of 30-50 mol % that possess liquid crystallinity	1342:1415	Finally, we unexpectedly proposed a novel cellulose/IL composite: the cellulose/IL mixtures of 30-50 mol % that possess liquid crystallinity are sufficiently hard to be moldable.
28117730	8	42	theme	cellulose/IL	1346:1357	arg1	moldable					1445:1452	moldable	1445:1452	moldable	1445:1452	Finally, we unexpectedly proposed a novel cellulose/IL composite: the cellulose/IL mixtures of 30-50 mol % that possess liquid crystallinity are sufficiently hard to be moldable.
28117730	2	43	theme	mol	392:394	arg1	%					396:396	40 mol %	389:396	40 mol %	389:396	Fibril structures of cellulose disappeared at 40 mol % of cellulose, which is a significantly higher concentration than the maximum concentration of dissolution (24-28 mol %) previously determined in this IL.
28117730	2	43	theme	mol	392:394	arg1	concentration					444:456	a significantly higher concentration	421:456	a significantly higher concentration than the maximum concentration of dissolution (24-28 mol %) previously determined in this IL	421:549	Fibril structures of cellulose disappeared at 40 mol % of cellulose, which is a significantly higher concentration than the maximum concentration of dissolution (24-28 mol %) previously determined in this IL.
28117730	2	43	theme	mol	392:394	arg1	cellulose					401:409	cellulose	401:409	cellulose	401:409	Fibril structures of cellulose disappeared at 40 mol % of cellulose, which is a significantly higher concentration than the maximum concentration of dissolution (24-28 mol %) previously determined in this IL.
28117730	2	44	theme	higher	437:442	arg1	%					396:396	40 mol %	389:396	40 mol %	389:396	Fibril structures of cellulose disappeared at 40 mol % of cellulose, which is a significantly higher concentration than the maximum concentration of dissolution (24-28 mol %) previously determined in this IL.
28117730	2	44	theme	higher	437:442	arg1	concentration					444:456	a significantly higher concentration	421:456	a significantly higher concentration than the maximum concentration of dissolution (24-28 mol %) previously determined in this IL	421:549	Fibril structures of cellulose disappeared at 40 mol % of cellulose, which is a significantly higher concentration than the maximum concentration of dissolution (24-28 mol %) previously determined in this IL.
28117730	2	44	theme	higher	437:442	arg1	cellulose					401:409	cellulose	401:409	cellulose	401:409	Fibril structures of cellulose disappeared at 40 mol % of cellulose, which is a significantly higher concentration than the maximum concentration of dissolution (24-28 mol %) previously determined in this IL.
28117730	1	45	theme	concentration	308:320	arg1	%					339:339	0-100 mol %	329:339	0-100 mol %	329:339	We investigated nano-structural changes of cellulose dissolved in 1-ethyl-3-methylimidazolium acetate-an ionic liquid (IL)-using a small angle X-ray scattering (SAXS) technique over the entire concentration range (0-100 mol %).
28117730	1	45	theme	concentration	308:320	arg1	range					322:326	the entire concentration range	297:326	the entire concentration range (0-100 mol %)	297:340	We investigated nano-structural changes of cellulose dissolved in 1-ethyl-3-methylimidazolium acetate-an ionic liquid (IL)-using a small angle X-ray scattering (SAXS) technique over the entire concentration range (0-100 mol %).
28117730	3	46	theme	anion	602:606	arg1	presence					586:593	the presence	582:593	the presence of the anion bridging, whereby an anion prefers to interact with multiple OH groups of different cellulose molecules at high concentrations, discovered in our recent work	582:764	This behavior is explained by the presence of the anion bridging, whereby an anion prefers to interact with multiple OH groups of different cellulose molecules at high concentrations, discovered in our recent work.
28117730	4	47	from	emergence	796:804	arg1	range					861:865	the concentration range	843:865	the concentration range of 30-80 mol %	843:880	Furthermore, we observed the emergence of two aggregated nano-structures in the concentration range of 30-80 mol %.
28117730	7	48	theme	dissolution	1233:1243	arg1	mechanism					1245:1253	the dissolution mechanism	1229:1253	the dissolution mechanism of cellulose in ILs	1229:1273	These results contribute to an understanding of the dissolution mechanism of cellulose in ILs.
28117730	4	49	theme	concentration	847:859	arg1	range					861:865	the concentration range	843:865	the concentration range of 30-80 mol %	843:880	Furthermore, we observed the emergence of two aggregated nano-structures in the concentration range of 30-80 mol %.
28117730	5	50	theme	cellulose	976:984	arg1	chain					986:990	cellulose chain entanglement	976:1003	cellulose chain entanglement	976:1003	The diameter of one structure was 12-20 nm, dependent on concentration, which is ascribed to cellulose chain entanglement.
28117730	6	51	theme	nanofibrillation	1163:1178	arg1	occurrence					1149:1158	the occurrence	1145:1158	the occurrence of nanofibrillation	1145:1178	In contrast, the other with 4.1 nm diameter exhibited concentration independence and is reminiscent of a cellulose microfibril, reflecting the occurrence of nanofibrillation.
28117730	6	52	theme	concentration	1060:1072	arg1	independence					1074:1085	concentration independence	1060:1085	concentration independence	1060:1085	In contrast, the other with 4.1 nm diameter exhibited concentration independence and is reminiscent of a cellulose microfibril, reflecting the occurrence of nanofibrillation.
28117730	8	53	theme	%	1381:1381	arg1	mixtures					1359:1366	the cellulose/IL mixtures	1342:1366	the cellulose/IL mixtures of 30-50 mol % that possess liquid crystallinity	1342:1415	Finally, we unexpectedly proposed a novel cellulose/IL composite: the cellulose/IL mixtures of 30-50 mol % that possess liquid crystallinity are sufficiently hard to be moldable.
28117730	8	53	theme	%	1381:1381	arg1	moldable					1445:1452	moldable	1445:1452	moldable	1445:1452	Finally, we unexpectedly proposed a novel cellulose/IL composite: the cellulose/IL mixtures of 30-50 mol % that possess liquid crystallinity are sufficiently hard to be moldable.
28117730	5	54	theme	dependent	927:935	arg1	diameter					887:894	The diameter	883:894	The diameter of one structure	883:911	The diameter of one structure was 12-20 nm, dependent on concentration, which is ascribed to cellulose chain entanglement.
28117730	5	54	theme	dependent	927:935	arg1	nm					923:924	12-20 nm	917:924	12-20 nm	917:924	The diameter of one structure was 12-20 nm, dependent on concentration, which is ascribed to cellulose chain entanglement.
28117730	7	55	from	ILs	1271:1273	arg1	understanding					1212:1224	an understanding	1209:1224	an understanding of the dissolution mechanism of cellulose in ILs	1209:1273	These results contribute to an understanding of the dissolution mechanism of cellulose in ILs.
28117730	2	56	theme	dissolution	492:502	arg1	concentration					475:487	the maximum concentration	463:487	the maximum concentration of dissolution (24-28 mol %)	463:516	Fibril structures of cellulose disappeared at 40 mol % of cellulose, which is a significantly higher concentration than the maximum concentration of dissolution (24-28 mol %) previously determined in this IL.
28117730	1	57	theme	-using	237:242	arg1	technique					282:290	1-ethyl-3-methylimidazolium acetate-an ionic liquid (IL)-using a small angle X-ray scattering (SAXS) technique	181:290	1-ethyl-3-methylimidazolium acetate-an ionic liquid (IL)-using a small angle X-ray scattering (SAXS) technique	181:290	We investigated nano-structural changes of cellulose dissolved in 1-ethyl-3-methylimidazolium acetate-an ionic liquid (IL)-using a small angle X-ray scattering (SAXS) technique over the entire concentration range (0-100 mol %).
28117730	5	58	theme	entanglement	992:1003	arg1	chain					986:990	cellulose chain entanglement	976:1003	cellulose chain entanglement	976:1003	The diameter of one structure was 12-20 nm, dependent on concentration, which is ascribed to cellulose chain entanglement.
28117730	8	59	theme	mol	1377:1379	arg1	%					1381:1381	30-50 mol %	1371:1381	30-50 mol %	1371:1381	Finally, we unexpectedly proposed a novel cellulose/IL composite: the cellulose/IL mixtures of 30-50 mol % that possess liquid crystallinity are sufficiently hard to be moldable.
28117730	1	60	theme	nano-structural	131:145	arg1	changes					147:153	nano-structural changes	131:153	nano-structural changes of cellulose dissolved in 1-ethyl-3-methylimidazolium acetate-an ionic liquid (IL)-using a small angle X-ray scattering (SAXS) technique	131:290	We investigated nano-structural changes of cellulose dissolved in 1-ethyl-3-methylimidazolium acetate-an ionic liquid (IL)-using a small angle X-ray scattering (SAXS) technique over the entire concentration range (0-100 mol %).
28117730	7	61	theme	cellulose	1258:1266	arg1	mechanism					1245:1253	the dissolution mechanism	1229:1253	the dissolution mechanism of cellulose in ILs	1229:1273	These results contribute to an understanding of the dissolution mechanism of cellulose in ILs.
28117730	2	62	theme	cellulose	401:409	arg1	%					396:396	40 mol %	389:396	40 mol %	389:396	Fibril structures of cellulose disappeared at 40 mol % of cellulose, which is a significantly higher concentration than the maximum concentration of dissolution (24-28 mol %) previously determined in this IL.
28117730	2	62	theme	cellulose	401:409	arg1	concentration					444:456	a significantly higher concentration	421:456	a significantly higher concentration than the maximum concentration of dissolution (24-28 mol %) previously determined in this IL	421:549	Fibril structures of cellulose disappeared at 40 mol % of cellulose, which is a significantly higher concentration than the maximum concentration of dissolution (24-28 mol %) previously determined in this IL.
28117730	2	62	theme	cellulose	401:409	arg1	cellulose					401:409	cellulose	401:409	cellulose	401:409	Fibril structures of cellulose disappeared at 40 mol % of cellulose, which is a significantly higher concentration than the maximum concentration of dissolution (24-28 mol %) previously determined in this IL.
28117730	6	63	theme	nm	1038:1039	arg1	diameter					1041:1048	4.1 nm diameter	1034:1048	4.1 nm diameter	1034:1048	In contrast, the other with 4.1 nm diameter exhibited concentration independence and is reminiscent of a cellulose microfibril, reflecting the occurrence of nanofibrillation.
28117730	1	64	theme	small	246:250	arg1	scattering					264:273	a small angle X-ray scattering	244:273	1-ethyl-3-methylimidazolium acetate-an ionic liquid (IL)-using a small angle X-ray scattering (SAXS) technique	181:290	We investigated nano-structural changes of cellulose dissolved in 1-ethyl-3-methylimidazolium acetate-an ionic liquid (IL)-using a small angle X-ray scattering (SAXS) technique over the entire concentration range (0-100 mol %).
28117730	1	64	theme	small	246:250	arg1	SAXS					276:279	SAXS	276:279	SAXS	276:279	We investigated nano-structural changes of cellulose dissolved in 1-ethyl-3-methylimidazolium acetate-an ionic liquid (IL)-using a small angle X-ray scattering (SAXS) technique over the entire concentration range (0-100 mol %).
28117730	3	65	theme	recent	754:759	arg1	work					761:764	our recent work	750:764	our recent work	750:764	This behavior is explained by the presence of the anion bridging, whereby an anion prefers to interact with multiple OH groups of different cellulose molecules at high concentrations, discovered in our recent work.
28117730	6	66	theme	4.1	1034:1036	arg1	nm					1038:1039	nm	1038:1039	nm	1038:1039	In contrast, the other with 4.1 nm diameter exhibited concentration independence and is reminiscent of a cellulose microfibril, reflecting the occurrence of nanofibrillation.
28117730	7	67	theme	mechanism	1245:1253	arg1	understanding					1212:1224	an understanding	1209:1224	an understanding of the dissolution mechanism of cellulose in ILs	1209:1273	These results contribute to an understanding of the dissolution mechanism of cellulose in ILs.
28117730	1	68	theme	angle	252:256	arg1	scattering					264:273	a small angle X-ray scattering	244:273	1-ethyl-3-methylimidazolium acetate-an ionic liquid (IL)-using a small angle X-ray scattering (SAXS) technique	181:290	We investigated nano-structural changes of cellulose dissolved in 1-ethyl-3-methylimidazolium acetate-an ionic liquid (IL)-using a small angle X-ray scattering (SAXS) technique over the entire concentration range (0-100 mol %).
28117730	1	68	theme	angle	252:256	arg1	SAXS					276:279	SAXS	276:279	SAXS	276:279	We investigated nano-structural changes of cellulose dissolved in 1-ethyl-3-methylimidazolium acetate-an ionic liquid (IL)-using a small angle X-ray scattering (SAXS) technique over the entire concentration range (0-100 mol %).
27653217	0	0	theme	structural	69:78	arg1	features					80:87	structural features	69:87	structural features of tea polysaccharides isolated from the crude polysaccharide	69:149	Hypolipidemic effects of crude green tea polysaccharide on rats, and structural features of tea polysaccharides isolated from the crude polysaccharide.
27653217	5	1	theme	arabinofuranosyl	1078:1093	arg1	residues					1095:1102	1,5-linked arabinofuranosyl residues	1067:1102	1,5-linked arabinofuranosyl residues	1067:1102	According to monosaccharide composition, methylation, and NMR analyses, NTPS-LP, NPTS-HH, and ATPS-MH were presumed to be starch, arabinogalactan with β-1,3-linked galactosyl backbone blanched at position 6 and with 1,5-linked arabinofuranosyl residues, and α-1,4-linked galacturonic acid backbone with arabinogalactan region, respectively.
27653217	2	2	theme	dietary	569:575	arg1	fat					577:579	dietary fat	569:579	dietary fat	569:579	Although crude TPS showed no effects on the serum lipid levels, it suppressed the liver lipid accumulation and increased the fecal excretion of dietary fat.
27653217	1	3	theme	Crude	152:156	arg1	polysaccharide					162:175	Crude tea polysaccharide	152:175	Crude tea polysaccharide (crude TPS)	152:187	Crude tea polysaccharide (crude TPS) was prepared from instant green tea by ethanol precipitation followed by ultrafiltration membrane treatment and its effects on blood lipid, liver lipid, and fecal lipid levels were examined with Sprague-Dawley rats fed a high-fat diet.
27653217	1	3	theme	Crude	152:156	arg1	TPS					184:186	crude TPS	178:186	crude TPS	178:186	Crude tea polysaccharide (crude TPS) was prepared from instant green tea by ethanol precipitation followed by ultrafiltration membrane treatment and its effects on blood lipid, liver lipid, and fecal lipid levels were examined with Sprague-Dawley rats fed a high-fat diet.
27653217	1	4	theme	ethanol	228:234	arg1	precipitation					236:248	ethanol precipitation	228:248	ethanol precipitation	228:248	Crude tea polysaccharide (crude TPS) was prepared from instant green tea by ethanol precipitation followed by ultrafiltration membrane treatment and its effects on blood lipid, liver lipid, and fecal lipid levels were examined with Sprague-Dawley rats fed a high-fat diet.
27653217	0	5	from	effects	14:20	arg1	rats					59:62	rats	59:62	rats	59:62	Hypolipidemic effects of crude green tea polysaccharide on rats, and structural features of tea polysaccharides isolated from the crude polysaccharide.
27653217	1	6	theme	high-fat	410:417	arg1	diet					419:422	a high-fat diet	408:422	a high-fat diet	408:422	Crude tea polysaccharide (crude TPS) was prepared from instant green tea by ethanol precipitation followed by ultrafiltration membrane treatment and its effects on blood lipid, liver lipid, and fecal lipid levels were examined with Sprague-Dawley rats fed a high-fat diet.
27653217	1	7	theme	tea	158:160	arg1	polysaccharide					162:175	Crude tea polysaccharide	152:175	Crude tea polysaccharide (crude TPS)	152:187	Crude tea polysaccharide (crude TPS) was prepared from instant green tea by ethanol precipitation followed by ultrafiltration membrane treatment and its effects on blood lipid, liver lipid, and fecal lipid levels were examined with Sprague-Dawley rats fed a high-fat diet.
27653217	1	7	theme	tea	158:160	arg1	TPS					184:186	crude TPS	178:186	crude TPS	178:186	Crude tea polysaccharide (crude TPS) was prepared from instant green tea by ethanol precipitation followed by ultrafiltration membrane treatment and its effects on blood lipid, liver lipid, and fecal lipid levels were examined with Sprague-Dawley rats fed a high-fat diet.
27653217	2	8	theme	fecal	550:554	arg1	excretion					556:564	the fecal excretion	546:564	the fecal excretion of dietary fat	546:579	Although crude TPS showed no effects on the serum lipid levels, it suppressed the liver lipid accumulation and increased the fecal excretion of dietary fat.
27653217	5	9	with	starch	973:978	arg1	backbone					1026:1033	β-1,3-linked galactosyl backbone	1002:1033	β-1,3-linked galactosyl backbone blanched at position 6	1002:1056	According to monosaccharide composition, methylation, and NMR analyses, NTPS-LP, NPTS-HH, and ATPS-MH were presumed to be starch, arabinogalactan with β-1,3-linked galactosyl backbone blanched at position 6 and with 1,5-linked arabinofuranosyl residues, and α-1,4-linked galacturonic acid backbone with arabinogalactan region, respectively.
27653217	5	9	with	starch	973:978	arg1	residues					1095:1102	1,5-linked arabinofuranosyl residues	1067:1102	1,5-linked arabinofuranosyl residues	1067:1102	According to monosaccharide composition, methylation, and NMR analyses, NTPS-LP, NPTS-HH, and ATPS-MH were presumed to be starch, arabinogalactan with β-1,3-linked galactosyl backbone blanched at position 6 and with 1,5-linked arabinofuranosyl residues, and α-1,4-linked galacturonic acid backbone with arabinogalactan region, respectively.
27653217	5	9	with	starch	973:978	arg1	region					1170:1175	arabinogalactan region	1154:1175	arabinogalactan region	1154:1175	According to monosaccharide composition, methylation, and NMR analyses, NTPS-LP, NPTS-HH, and ATPS-MH were presumed to be starch, arabinogalactan with β-1,3-linked galactosyl backbone blanched at position 6 and with 1,5-linked arabinofuranosyl residues, and α-1,4-linked galacturonic acid backbone with arabinogalactan region, respectively.
27653217	1	10	from	effects	305:311	arg1	levels					358:363	fecal lipid levels	346:363	fecal lipid levels	346:363	Crude tea polysaccharide (crude TPS) was prepared from instant green tea by ethanol precipitation followed by ultrafiltration membrane treatment and its effects on blood lipid, liver lipid, and fecal lipid levels were examined with Sprague-Dawley rats fed a high-fat diet.
27653217	1	10	from	effects	305:311	arg1	lipid					335:339	liver lipid	329:339	liver lipid	329:339	Crude tea polysaccharide (crude TPS) was prepared from instant green tea by ethanol precipitation followed by ultrafiltration membrane treatment and its effects on blood lipid, liver lipid, and fecal lipid levels were examined with Sprague-Dawley rats fed a high-fat diet.
27653217	1	10	from	effects	305:311	arg1	lipid					322:326	blood lipid	316:326	blood lipid	316:326	Crude tea polysaccharide (crude TPS) was prepared from instant green tea by ethanol precipitation followed by ultrafiltration membrane treatment and its effects on blood lipid, liver lipid, and fecal lipid levels were examined with Sprague-Dawley rats fed a high-fat diet.
27653217	0	11	attach	isolated	112:119	arg2	polysaccharides					96:110	tea polysaccharides	92:110	tea polysaccharides isolated from the crude polysaccharide	92:149	Hypolipidemic effects of crude green tea polysaccharide on rats, and structural features of tea polysaccharides isolated from the crude polysaccharide.
27653217	0	11	attach	isolated	112:119	arg1	polysaccharide					136:149	the crude polysaccharide	126:149	the crude polysaccharide	126:149	Hypolipidemic effects of crude green tea polysaccharide on rats, and structural features of tea polysaccharides isolated from the crude polysaccharide.
27653217	5	12	with	arabinogalactan	981:995	arg1	backbone					1026:1033	β-1,3-linked galactosyl backbone	1002:1033	β-1,3-linked galactosyl backbone blanched at position 6	1002:1056	According to monosaccharide composition, methylation, and NMR analyses, NTPS-LP, NPTS-HH, and ATPS-MH were presumed to be starch, arabinogalactan with β-1,3-linked galactosyl backbone blanched at position 6 and with 1,5-linked arabinofuranosyl residues, and α-1,4-linked galacturonic acid backbone with arabinogalactan region, respectively.
27653217	5	12	with	arabinogalactan	981:995	arg1	residues					1095:1102	1,5-linked arabinofuranosyl residues	1067:1102	1,5-linked arabinofuranosyl residues	1067:1102	According to monosaccharide composition, methylation, and NMR analyses, NTPS-LP, NPTS-HH, and ATPS-MH were presumed to be starch, arabinogalactan with β-1,3-linked galactosyl backbone blanched at position 6 and with 1,5-linked arabinofuranosyl residues, and α-1,4-linked galacturonic acid backbone with arabinogalactan region, respectively.
27653217	5	12	with	arabinogalactan	981:995	arg1	region					1170:1175	arabinogalactan region	1154:1175	arabinogalactan region	1154:1175	According to monosaccharide composition, methylation, and NMR analyses, NTPS-LP, NPTS-HH, and ATPS-MH were presumed to be starch, arabinogalactan with β-1,3-linked galactosyl backbone blanched at position 6 and with 1,5-linked arabinofuranosyl residues, and α-1,4-linked galacturonic acid backbone with arabinogalactan region, respectively.
27653217	0	13	theme	polysaccharides	96:110	arg1	effects					14:20	Hypolipidemic effects	0:20	Hypolipidemic effects of crude green tea polysaccharide on rats	0:62	Hypolipidemic effects of crude green tea polysaccharide on rats, and structural features of tea polysaccharides isolated from the crude polysaccharide.
27653217	0	13	theme	polysaccharides	96:110	arg1	features					80:87	structural features	69:87	structural features of tea polysaccharides isolated from the crude polysaccharide	69:149	Hypolipidemic effects of crude green tea polysaccharide on rats, and structural features of tea polysaccharides isolated from the crude polysaccharide.
27653217	5	14	theme	monosaccharide	864:877	arg1	composition					879:889	monosaccharide composition	864:889	monosaccharide composition	864:889	According to monosaccharide composition, methylation, and NMR analyses, NTPS-LP, NPTS-HH, and ATPS-MH were presumed to be starch, arabinogalactan with β-1,3-linked galactosyl backbone blanched at position 6 and with 1,5-linked arabinofuranosyl residues, and α-1,4-linked galacturonic acid backbone with arabinogalactan region, respectively.
27653217	0	15	theme	tea	92:94	arg1	polysaccharides					96:110	tea polysaccharides	92:110	tea polysaccharides isolated from the crude polysaccharide	92:149	Hypolipidemic effects of crude green tea polysaccharide on rats, and structural features of tea polysaccharides isolated from the crude polysaccharide.
27653217	1	16	theme	fecal	346:350	arg1	levels					358:363	fecal lipid levels	346:363	fecal lipid levels	346:363	Crude tea polysaccharide (crude TPS) was prepared from instant green tea by ethanol precipitation followed by ultrafiltration membrane treatment and its effects on blood lipid, liver lipid, and fecal lipid levels were examined with Sprague-Dawley rats fed a high-fat diet.
27653217	0	17	theme	Hypolipidemic	0:12	arg1	effects					14:20	Hypolipidemic effects	0:20	Hypolipidemic effects of crude green tea polysaccharide on rats	0:62	Hypolipidemic effects of crude green tea polysaccharide on rats, and structural features of tea polysaccharides isolated from the crude polysaccharide.
27653217	4	18	dep	polysaccharides	759:773	arg1	polysaccharides					759:773	neutral tea polysaccharides	747:773	neutral tea polysaccharides (NTPS-LP and NTPS-HH)	747:795	After separation of crude TPS by DEAE-cellulose and gel-filtration column chromatography, two kinds of neutral tea polysaccharides (NTPS-LP and NTPS-HH) and an acidic polysaccharide (ATPS-MH) were obtained.
27653217	4	18	dep	polysaccharides	759:773	arg1	NTPS-HH					788:794	NTPS-HH	788:794	NTPS-HH	788:794	After separation of crude TPS by DEAE-cellulose and gel-filtration column chromatography, two kinds of neutral tea polysaccharides (NTPS-LP and NTPS-HH) and an acidic polysaccharide (ATPS-MH) were obtained.
27653217	4	18	dep	polysaccharides	759:773	arg1	NTPS-LP					776:782	NTPS-LP	776:782	NTPS-LP	776:782	After separation of crude TPS by DEAE-cellulose and gel-filtration column chromatography, two kinds of neutral tea polysaccharides (NTPS-LP and NTPS-HH) and an acidic polysaccharide (ATPS-MH) were obtained.
27653217	1	19	theme	lipid	352:356	arg1	levels					358:363	fecal lipid levels	346:363	fecal lipid levels	346:363	Crude tea polysaccharide (crude TPS) was prepared from instant green tea by ethanol precipitation followed by ultrafiltration membrane treatment and its effects on blood lipid, liver lipid, and fecal lipid levels were examined with Sprague-Dawley rats fed a high-fat diet.
27653217	4	20	theme	polysaccharide	811:824	arg1	kinds					738:742	two kinds	734:742	two kinds of neutral tea polysaccharides (NTPS-LP and NTPS-HH) and an acidic polysaccharide (ATPS-MH)	734:834	After separation of crude TPS by DEAE-cellulose and gel-filtration column chromatography, two kinds of neutral tea polysaccharides (NTPS-LP and NTPS-HH) and an acidic polysaccharide (ATPS-MH) were obtained.
27653217	4	21	theme	gel-filtration	696:709	arg1	chromatography					718:731	DEAE-cellulose and gel-filtration column chromatography	677:731	chromatography	718:731	After separation of crude TPS by DEAE-cellulose and gel-filtration column chromatography, two kinds of neutral tea polysaccharides (NTPS-LP and NTPS-HH) and an acidic polysaccharide (ATPS-MH) were obtained.
27653217	5	22	theme	acid	1135:1138	arg1	backbone					1140:1147	α-1,4-linked galacturonic acid backbone	1109:1147	α-1,4-linked galacturonic acid backbone with arabinogalactan region	1109:1175	According to monosaccharide composition, methylation, and NMR analyses, NTPS-LP, NPTS-HH, and ATPS-MH were presumed to be starch, arabinogalactan with β-1,3-linked galactosyl backbone blanched at position 6 and with 1,5-linked arabinofuranosyl residues, and α-1,4-linked galacturonic acid backbone with arabinogalactan region, respectively.
27653217	1	23	theme	crude	178:182	arg1	polysaccharide					162:175	Crude tea polysaccharide	152:175	Crude tea polysaccharide (crude TPS)	152:187	Crude tea polysaccharide (crude TPS) was prepared from instant green tea by ethanol precipitation followed by ultrafiltration membrane treatment and its effects on blood lipid, liver lipid, and fecal lipid levels were examined with Sprague-Dawley rats fed a high-fat diet.
27653217	1	23	theme	crude	178:182	arg1	TPS					184:186	crude TPS	178:186	crude TPS	178:186	Crude tea polysaccharide (crude TPS) was prepared from instant green tea by ethanol precipitation followed by ultrafiltration membrane treatment and its effects on blood lipid, liver lipid, and fecal lipid levels were examined with Sprague-Dawley rats fed a high-fat diet.
27653217	1	24	theme	liver	329:333	arg1	lipid					335:339	liver lipid	329:339	liver lipid	329:339	Crude tea polysaccharide (crude TPS) was prepared from instant green tea by ethanol precipitation followed by ultrafiltration membrane treatment and its effects on blood lipid, liver lipid, and fecal lipid levels were examined with Sprague-Dawley rats fed a high-fat diet.
27653217	1	25	theme	ultrafiltration	262:276	arg1	treatment					287:295	ultrafiltration membrane treatment	262:295	ultrafiltration membrane treatment	262:295	Crude tea polysaccharide (crude TPS) was prepared from instant green tea by ethanol precipitation followed by ultrafiltration membrane treatment and its effects on blood lipid, liver lipid, and fecal lipid levels were examined with Sprague-Dawley rats fed a high-fat diet.
27653217	2	26	theme	fat	577:579	arg1	excretion					556:564	the fecal excretion	546:564	the fecal excretion of dietary fat	546:579	Although crude TPS showed no effects on the serum lipid levels, it suppressed the liver lipid accumulation and increased the fecal excretion of dietary fat.
27653217	5	27	with	backbone	1140:1147	arg1	backbone					1026:1033	β-1,3-linked galactosyl backbone	1002:1033	β-1,3-linked galactosyl backbone blanched at position 6	1002:1056	According to monosaccharide composition, methylation, and NMR analyses, NTPS-LP, NPTS-HH, and ATPS-MH were presumed to be starch, arabinogalactan with β-1,3-linked galactosyl backbone blanched at position 6 and with 1,5-linked arabinofuranosyl residues, and α-1,4-linked galacturonic acid backbone with arabinogalactan region, respectively.
27653217	5	27	with	backbone	1140:1147	arg1	residues					1095:1102	1,5-linked arabinofuranosyl residues	1067:1102	1,5-linked arabinofuranosyl residues	1067:1102	According to monosaccharide composition, methylation, and NMR analyses, NTPS-LP, NPTS-HH, and ATPS-MH were presumed to be starch, arabinogalactan with β-1,3-linked galactosyl backbone blanched at position 6 and with 1,5-linked arabinofuranosyl residues, and α-1,4-linked galacturonic acid backbone with arabinogalactan region, respectively.
27653217	5	27	with	backbone	1140:1147	arg1	region					1170:1175	arabinogalactan region	1154:1175	arabinogalactan region	1154:1175	According to monosaccharide composition, methylation, and NMR analyses, NTPS-LP, NPTS-HH, and ATPS-MH were presumed to be starch, arabinogalactan with β-1,3-linked galactosyl backbone blanched at position 6 and with 1,5-linked arabinofuranosyl residues, and α-1,4-linked galacturonic acid backbone with arabinogalactan region, respectively.
27653217	0	28	theme	crude	25:29	arg1	polysaccharide					41:54	crude green tea polysaccharide	25:54	crude green tea polysaccharide	25:54	Hypolipidemic effects of crude green tea polysaccharide on rats, and structural features of tea polysaccharides isolated from the crude polysaccharide.
27653217	1	29	theme	membrane	278:285	arg1	treatment					287:295	ultrafiltration membrane treatment	262:295	ultrafiltration membrane treatment	262:295	Crude tea polysaccharide (crude TPS) was prepared from instant green tea by ethanol precipitation followed by ultrafiltration membrane treatment and its effects on blood lipid, liver lipid, and fecal lipid levels were examined with Sprague-Dawley rats fed a high-fat diet.
27653217	2	30	theme	lipid	513:517	arg1	accumulation					519:530	the liver lipid accumulation	503:530	the liver lipid accumulation	503:530	Although crude TPS showed no effects on the serum lipid levels, it suppressed the liver lipid accumulation and increased the fecal excretion of dietary fat.
27653217	5	31	theme	NMR	909:911	arg1	analyses					913:920	NMR analyses	909:920	NMR analyses	909:920	According to monosaccharide composition, methylation, and NMR analyses, NTPS-LP, NPTS-HH, and ATPS-MH were presumed to be starch, arabinogalactan with β-1,3-linked galactosyl backbone blanched at position 6 and with 1,5-linked arabinofuranosyl residues, and α-1,4-linked galacturonic acid backbone with arabinogalactan region, respectively.
27653217	5	32	theme	β-1,3-linked	1002:1013	arg1	backbone					1026:1033	β-1,3-linked galactosyl backbone	1002:1033	β-1,3-linked galactosyl backbone blanched at position 6	1002:1056	According to monosaccharide composition, methylation, and NMR analyses, NTPS-LP, NPTS-HH, and ATPS-MH were presumed to be starch, arabinogalactan with β-1,3-linked galactosyl backbone blanched at position 6 and with 1,5-linked arabinofuranosyl residues, and α-1,4-linked galacturonic acid backbone with arabinogalactan region, respectively.
27653217	5	33	theme	arabinogalactan	1154:1168	arg1	region					1170:1175	arabinogalactan region	1154:1175	arabinogalactan region	1154:1175	According to monosaccharide composition, methylation, and NMR analyses, NTPS-LP, NPTS-HH, and ATPS-MH were presumed to be starch, arabinogalactan with β-1,3-linked galactosyl backbone blanched at position 6 and with 1,5-linked arabinofuranosyl residues, and α-1,4-linked galacturonic acid backbone with arabinogalactan region, respectively.
27653217	2	34	theme	liver	507:511	arg1	accumulation					519:530	the liver lipid accumulation	503:530	the liver lipid accumulation	503:530	Although crude TPS showed no effects on the serum lipid levels, it suppressed the liver lipid accumulation and increased the fecal excretion of dietary fat.
27653217	5	35	theme	galactosyl	1015:1024	arg1	backbone					1026:1033	β-1,3-linked galactosyl backbone	1002:1033	β-1,3-linked galactosyl backbone blanched at position 6	1002:1056	According to monosaccharide composition, methylation, and NMR analyses, NTPS-LP, NPTS-HH, and ATPS-MH were presumed to be starch, arabinogalactan with β-1,3-linked galactosyl backbone blanched at position 6 and with 1,5-linked arabinofuranosyl residues, and α-1,4-linked galacturonic acid backbone with arabinogalactan region, respectively.
27653217	4	36	theme	column	711:716	arg1	chromatography					718:731	DEAE-cellulose and gel-filtration column chromatography	677:731	chromatography	718:731	After separation of crude TPS by DEAE-cellulose and gel-filtration column chromatography, two kinds of neutral tea polysaccharides (NTPS-LP and NTPS-HH) and an acidic polysaccharide (ATPS-MH) were obtained.
27653217	5	37	link	1,5-linked	1067:1076	arg1	residues					1095:1102	1,5-linked arabinofuranosyl residues	1067:1102	1,5-linked arabinofuranosyl residues	1067:1102	According to monosaccharide composition, methylation, and NMR analyses, NTPS-LP, NPTS-HH, and ATPS-MH were presumed to be starch, arabinogalactan with β-1,3-linked galactosyl backbone blanched at position 6 and with 1,5-linked arabinofuranosyl residues, and α-1,4-linked galacturonic acid backbone with arabinogalactan region, respectively.
27653217	0	38	theme	tea	37:39	arg1	polysaccharide					41:54	crude green tea polysaccharide	25:54	crude green tea polysaccharide	25:54	Hypolipidemic effects of crude green tea polysaccharide on rats, and structural features of tea polysaccharides isolated from the crude polysaccharide.
27653217	3	39	theme	TPS	621:623	arg1	features					603:610	the structural features	588:610	the structural features of crude TPS	588:623	Then, the structural features of crude TPS were investigated.
27653217	0	40	theme	crude	130:134	arg1	polysaccharide					136:149	the crude polysaccharide	126:149	the crude polysaccharide	126:149	Hypolipidemic effects of crude green tea polysaccharide on rats, and structural features of tea polysaccharides isolated from the crude polysaccharide.
27653217	2	41	from	effects	454:460	arg1	levels					481:486	the serum lipid levels	465:486	the serum lipid levels	465:486	Although crude TPS showed no effects on the serum lipid levels, it suppressed the liver lipid accumulation and increased the fecal excretion of dietary fat.
27653217	0	42	theme	green	31:35	arg1	polysaccharide					41:54	crude green tea polysaccharide	25:54	crude green tea polysaccharide	25:54	Hypolipidemic effects of crude green tea polysaccharide on rats, and structural features of tea polysaccharides isolated from the crude polysaccharide.
27653217	2	43	theme	lipid	475:479	arg1	levels					481:486	the serum lipid levels	465:486	the serum lipid levels	465:486	Although crude TPS showed no effects on the serum lipid levels, it suppressed the liver lipid accumulation and increased the fecal excretion of dietary fat.
27653217	5	44	theme	α-1,4-linked	1109:1120	arg1	backbone					1140:1147	α-1,4-linked galacturonic acid backbone	1109:1147	α-1,4-linked galacturonic acid backbone with arabinogalactan region	1109:1175	According to monosaccharide composition, methylation, and NMR analyses, NTPS-LP, NPTS-HH, and ATPS-MH were presumed to be starch, arabinogalactan with β-1,3-linked galactosyl backbone blanched at position 6 and with 1,5-linked arabinofuranosyl residues, and α-1,4-linked galacturonic acid backbone with arabinogalactan region, respectively.
27653217	3	45	theme	structural	592:601	arg1	features					603:610	the structural features	588:610	the structural features of crude TPS	588:623	Then, the structural features of crude TPS were investigated.
27653217	4	46	theme	acidic	804:809	arg1	ATPS-MH					827:833	ATPS-MH	827:833	ATPS-MH	827:833	After separation of crude TPS by DEAE-cellulose and gel-filtration column chromatography, two kinds of neutral tea polysaccharides (NTPS-LP and NTPS-HH) and an acidic polysaccharide (ATPS-MH) were obtained.
27653217	4	46	theme	acidic	804:809	arg1	polysaccharide					811:824	an acidic polysaccharide	801:824	an acidic polysaccharide (ATPS-MH)	801:834	After separation of crude TPS by DEAE-cellulose and gel-filtration column chromatography, two kinds of neutral tea polysaccharides (NTPS-LP and NTPS-HH) and an acidic polysaccharide (ATPS-MH) were obtained.
27653217	4	47	theme	TPS	670:672	arg1	separation					650:659	separation	650:659	separation of crude TPS by DEAE-cellulose and gel-filtration column chromatography	650:731	After separation of crude TPS by DEAE-cellulose and gel-filtration column chromatography, two kinds of neutral tea polysaccharides (NTPS-LP and NTPS-HH) and an acidic polysaccharide (ATPS-MH) were obtained.
27653217	0	48	theme	polysaccharide	41:54	arg1	effects					14:20	Hypolipidemic effects	0:20	Hypolipidemic effects of crude green tea polysaccharide on rats	0:62	Hypolipidemic effects of crude green tea polysaccharide on rats, and structural features of tea polysaccharides isolated from the crude polysaccharide.
27653217	0	48	theme	polysaccharide	41:54	arg1	features					80:87	structural features	69:87	structural features of tea polysaccharides isolated from the crude polysaccharide	69:149	Hypolipidemic effects of crude green tea polysaccharide on rats, and structural features of tea polysaccharides isolated from the crude polysaccharide.
27653217	5	49	theme	galacturonic	1122:1133	arg1	backbone					1140:1147	α-1,4-linked galacturonic acid backbone	1109:1147	α-1,4-linked galacturonic acid backbone with arabinogalactan region	1109:1175	According to monosaccharide composition, methylation, and NMR analyses, NTPS-LP, NPTS-HH, and ATPS-MH were presumed to be starch, arabinogalactan with β-1,3-linked galactosyl backbone blanched at position 6 and with 1,5-linked arabinofuranosyl residues, and α-1,4-linked galacturonic acid backbone with arabinogalactan region, respectively.
27653217	1	50	theme	Sprague-Dawley	384:397	arg1	rats					399:402	Sprague-Dawley rats	384:402	Sprague-Dawley rats fed a high-fat diet	384:422	Crude tea polysaccharide (crude TPS) was prepared from instant green tea by ethanol precipitation followed by ultrafiltration membrane treatment and its effects on blood lipid, liver lipid, and fecal lipid levels were examined with Sprague-Dawley rats fed a high-fat diet.
27653217	4	51	theme	crude	664:668	arg1	TPS					670:672	crude TPS	664:672	crude TPS	664:672	After separation of crude TPS by DEAE-cellulose and gel-filtration column chromatography, two kinds of neutral tea polysaccharides (NTPS-LP and NTPS-HH) and an acidic polysaccharide (ATPS-MH) were obtained.
27653217	1	52	theme	blood	316:320	arg1	lipid					322:326	blood lipid	316:326	blood lipid	316:326	Crude tea polysaccharide (crude TPS) was prepared from instant green tea by ethanol precipitation followed by ultrafiltration membrane treatment and its effects on blood lipid, liver lipid, and fecal lipid levels were examined with Sprague-Dawley rats fed a high-fat diet.
27653217	4	53	theme	tea	755:757	arg1	polysaccharides					759:773	neutral tea polysaccharides	747:773	neutral tea polysaccharides (NTPS-LP and NTPS-HH)	747:795	After separation of crude TPS by DEAE-cellulose and gel-filtration column chromatography, two kinds of neutral tea polysaccharides (NTPS-LP and NTPS-HH) and an acidic polysaccharide (ATPS-MH) were obtained.
27653217	4	53	theme	tea	755:757	arg1	NTPS-HH					788:794	NTPS-HH	788:794	NTPS-HH	788:794	After separation of crude TPS by DEAE-cellulose and gel-filtration column chromatography, two kinds of neutral tea polysaccharides (NTPS-LP and NTPS-HH) and an acidic polysaccharide (ATPS-MH) were obtained.
27653217	4	53	theme	tea	755:757	arg1	NTPS-LP					776:782	NTPS-LP	776:782	NTPS-LP	776:782	After separation of crude TPS by DEAE-cellulose and gel-filtration column chromatography, two kinds of neutral tea polysaccharides (NTPS-LP and NTPS-HH) and an acidic polysaccharide (ATPS-MH) were obtained.
27653217	1	54	dep	prepared	193:200	arg1	followed					250:257	followed	250:257	followed by ultrafiltration membrane treatment	250:295	Crude tea polysaccharide (crude TPS) was prepared from instant green tea by ethanol precipitation followed by ultrafiltration membrane treatment and its effects on blood lipid, liver lipid, and fecal lipid levels were examined with Sprague-Dawley rats fed a high-fat diet.
27653217	5	55	link	α-1,4-linked	1109:1120	arg1	backbone					1140:1147	α-1,4-linked galacturonic acid backbone	1109:1147	α-1,4-linked galacturonic acid backbone with arabinogalactan region	1109:1175	According to monosaccharide composition, methylation, and NMR analyses, NTPS-LP, NPTS-HH, and ATPS-MH were presumed to be starch, arabinogalactan with β-1,3-linked galactosyl backbone blanched at position 6 and with 1,5-linked arabinofuranosyl residues, and α-1,4-linked galacturonic acid backbone with arabinogalactan region, respectively.
27653217	4	56	theme	DEAE-cellulose	677:690	arg1	chromatography					718:731	DEAE-cellulose and gel-filtration column chromatography	677:731	chromatography	718:731	After separation of crude TPS by DEAE-cellulose and gel-filtration column chromatography, two kinds of neutral tea polysaccharides (NTPS-LP and NTPS-HH) and an acidic polysaccharide (ATPS-MH) were obtained.
27653217	5	57	link	β-1,3-linked	1002:1013	arg1	backbone					1026:1033	β-1,3-linked galactosyl backbone	1002:1033	β-1,3-linked galactosyl backbone blanched at position 6	1002:1056	According to monosaccharide composition, methylation, and NMR analyses, NTPS-LP, NPTS-HH, and ATPS-MH were presumed to be starch, arabinogalactan with β-1,3-linked galactosyl backbone blanched at position 6 and with 1,5-linked arabinofuranosyl residues, and α-1,4-linked galacturonic acid backbone with arabinogalactan region, respectively.
27653217	1	58	theme	instant	207:213	arg1	tea					221:223	instant green tea	207:223	instant green tea by ethanol precipitation	207:248	Crude tea polysaccharide (crude TPS) was prepared from instant green tea by ethanol precipitation followed by ultrafiltration membrane treatment and its effects on blood lipid, liver lipid, and fecal lipid levels were examined with Sprague-Dawley rats fed a high-fat diet.
27653217	3	59	theme	crude	615:619	arg1	TPS					621:623	crude TPS	615:623	crude TPS	615:623	Then, the structural features of crude TPS were investigated.
27653217	4	60	theme	neutral	747:753	arg1	polysaccharides					759:773	neutral tea polysaccharides	747:773	neutral tea polysaccharides (NTPS-LP and NTPS-HH)	747:795	After separation of crude TPS by DEAE-cellulose and gel-filtration column chromatography, two kinds of neutral tea polysaccharides (NTPS-LP and NTPS-HH) and an acidic polysaccharide (ATPS-MH) were obtained.
27653217	4	60	theme	neutral	747:753	arg1	NTPS-HH					788:794	NTPS-HH	788:794	NTPS-HH	788:794	After separation of crude TPS by DEAE-cellulose and gel-filtration column chromatography, two kinds of neutral tea polysaccharides (NTPS-LP and NTPS-HH) and an acidic polysaccharide (ATPS-MH) were obtained.
27653217	4	60	theme	neutral	747:753	arg1	NTPS-LP					776:782	NTPS-LP	776:782	NTPS-LP	776:782	After separation of crude TPS by DEAE-cellulose and gel-filtration column chromatography, two kinds of neutral tea polysaccharides (NTPS-LP and NTPS-HH) and an acidic polysaccharide (ATPS-MH) were obtained.
27653217	2	61	theme	crude	434:438	arg1	TPS					440:442	crude TPS	434:442	crude TPS	434:442	Although crude TPS showed no effects on the serum lipid levels, it suppressed the liver lipid accumulation and increased the fecal excretion of dietary fat.
27653217	1	62	theme	green	215:219	arg1	tea					221:223	instant green tea	207:223	instant green tea by ethanol precipitation	207:248	Crude tea polysaccharide (crude TPS) was prepared from instant green tea by ethanol precipitation followed by ultrafiltration membrane treatment and its effects on blood lipid, liver lipid, and fecal lipid levels were examined with Sprague-Dawley rats fed a high-fat diet.
27653217	2	63	theme	serum	469:473	arg1	levels					481:486	the serum lipid levels	465:486	the serum lipid levels	465:486	Although crude TPS showed no effects on the serum lipid levels, it suppressed the liver lipid accumulation and increased the fecal excretion of dietary fat.
27653217	0	64	from	features	80:87	arg1	rats					59:62	rats	59:62	rats	59:62	Hypolipidemic effects of crude green tea polysaccharide on rats, and structural features of tea polysaccharides isolated from the crude polysaccharide.
27653217	5	65	theme	1,5-linked	1067:1076	arg1	residues					1095:1102	1,5-linked arabinofuranosyl residues	1067:1102	1,5-linked arabinofuranosyl residues	1067:1102	According to monosaccharide composition, methylation, and NMR analyses, NTPS-LP, NPTS-HH, and ATPS-MH were presumed to be starch, arabinogalactan with β-1,3-linked galactosyl backbone blanched at position 6 and with 1,5-linked arabinofuranosyl residues, and α-1,4-linked galacturonic acid backbone with arabinogalactan region, respectively.
27653217	4	66	theme	polysaccharides	759:773	arg1	kinds					738:742	two kinds	734:742	two kinds of neutral tea polysaccharides (NTPS-LP and NTPS-HH) and an acidic polysaccharide (ATPS-MH)	734:834	After separation of crude TPS by DEAE-cellulose and gel-filtration column chromatography, two kinds of neutral tea polysaccharides (NTPS-LP and NTPS-HH) and an acidic polysaccharide (ATPS-MH) were obtained.
26280087	3	0	theme	cell	686:689	arg1	membrane					691:698	the cell membrane	682:698	the cell membrane	682:698	A cytosolic O-GlcNAc modification is normally terminal and does not extend to produce a polysaccharide, but O-GlcNAc on an HLA peptide presents a special case because the loaded HLA class I complex traffics through the endoplasmic reticulum and Golgi apparatus on its way to the cell membrane and is hence exposed to glycosyltransferases.
26280087	1	1	theme	I-bound	154:160	arg1	peptides					162:169	glycosylated human leukocyte antigen (HLA) class I-bound peptides	105:169	glycosylated human leukocyte antigen (HLA) class I-bound peptides	105:169	We report unexpected mass spectrometric observations of glycosylated human leukocyte antigen (HLA) class I-bound peptides.
26280087	0	2	from	O-GlcNAc	9:16	arg1	Peptides					39:46	HLA Class-I-Bound Peptides	21:46	HLA Class-I-Bound Peptides	21:46	Extended O-GlcNAc on HLA Class-I-Bound Peptides.
26280087	4	3	theme	glycopeptides	832:844	arg1	presentation					800:811	the first time natural HLA class I presentation	765:811	the first time natural HLA class I presentation of O- and N-linked glycopeptides derived from membrane proteins	765:875	We also report for the first time natural HLA class I presentation of O- and N-linked glycopeptides derived from membrane proteins.
26280087	5	4	theme	HLA	878:880	arg1	class					882:886	HLA class I	878:888	HLA class I peptides with centrally located oligosaccharides	878:937	HLA class I peptides with centrally located oligosaccharides have been shown to be immunogenic and may thus be important targets for immune surveillance.
26280087	1	5	theme	peptides	162:169	arg1	observations					89:100	unexpected mass spectrometric observations	59:100	unexpected mass spectrometric observations of glycosylated human leukocyte antigen (HLA) class I-bound peptides	59:169	We report unexpected mass spectrometric observations of glycosylated human leukocyte antigen (HLA) class I-bound peptides.
26280087	4	6	theme	membrane	859:866	arg1	proteins					868:875	membrane proteins	859:875	membrane proteins	859:875	We also report for the first time natural HLA class I presentation of O- and N-linked glycopeptides derived from membrane proteins.
26280087	1	7	theme	human	118:122	arg1	HLA					143:145	HLA	143:145	HLA	143:145	We report unexpected mass spectrometric observations of glycosylated human leukocyte antigen (HLA) class I-bound peptides.
26280087	1	7	theme	human	118:122	arg1	antigen					134:140	human leukocyte antigen	118:140	glycosylated human leukocyte antigen (HLA) class I-bound peptides	105:169	We report unexpected mass spectrometric observations of glycosylated human leukocyte antigen (HLA) class I-bound peptides.
26280087	3	8	from	O-GlcNAc	515:522	arg1	peptide					534:540	an HLA peptide	527:540	an HLA peptide	527:540	A cytosolic O-GlcNAc modification is normally terminal and does not extend to produce a polysaccharide, but O-GlcNAc on an HLA peptide presents a special case because the loaded HLA class I complex traffics through the endoplasmic reticulum and Golgi apparatus on its way to the cell membrane and is hence exposed to glycosyltransferases.
26280087	1	9	theme	unexpected	59:68	arg1	observations					89:100	unexpected mass spectrometric observations	59:100	unexpected mass spectrometric observations of glycosylated human leukocyte antigen (HLA) class I-bound peptides	59:169	We report unexpected mass spectrometric observations of glycosylated human leukocyte antigen (HLA) class I-bound peptides.
26280087	4	10	theme	O-	816:817	arg1	presentation					800:811	the first time natural HLA class I presentation	765:811	the first time natural HLA class I presentation of O- and N-linked glycopeptides derived from membrane proteins	765:875	We also report for the first time natural HLA class I presentation of O- and N-linked glycopeptides derived from membrane proteins.
26280087	1	11	theme	mass	70:73	arg1	observations					89:100	unexpected mass spectrometric observations	59:100	unexpected mass spectrometric observations of glycosylated human leukocyte antigen (HLA) class I-bound peptides	59:169	We report unexpected mass spectrometric observations of glycosylated human leukocyte antigen (HLA) class I-bound peptides.
26280087	4	12	gly	glycopeptides	832:844	arg2	glycopeptides					832:844	N-linked glycopeptides	823:844	N-linked glycopeptides derived from membrane proteins	823:875	We also report for the first time natural HLA class I presentation of O- and N-linked glycopeptides derived from membrane proteins.
26280087	1	13	gly	glycosylated	105:116	arg1	peptides					162:169	glycosylated human leukocyte antigen (HLA) class I-bound peptides	105:169	glycosylated human leukocyte antigen (HLA) class I-bound peptides	105:169	We report unexpected mass spectrometric observations of glycosylated human leukocyte antigen (HLA) class I-bound peptides.
26280087	5	14	theme	class	882:886	arg1	peptides					890:897	HLA class I peptides	878:897	HLA class I peptides with centrally located oligosaccharides	878:937	HLA class I peptides with centrally located oligosaccharides have been shown to be immunogenic and may thus be important targets for immune surveillance.
26280087	4	15	link	N-linked	823:830	arg1	glycopeptides					832:844	N-linked glycopeptides	823:844	N-linked glycopeptides derived from membrane proteins	823:875	We also report for the first time natural HLA class I presentation of O- and N-linked glycopeptides derived from membrane proteins.
26280087	1	16	theme	spectrometric	75:87	arg1	observations					89:100	unexpected mass spectrometric observations	59:100	unexpected mass spectrometric observations of glycosylated human leukocyte antigen (HLA) class I-bound peptides	59:169	We report unexpected mass spectrometric observations of glycosylated human leukocyte antigen (HLA) class I-bound peptides.
26280087	2	17	theme	GalNAc-initiated	379:394	arg1	O-glycans					396:404	GalNAc-initiated O-glycans	379:404	O-GlcNAc's rather than GalNAc-initiated O-glycans	356:404	Complemented by molecular modeling, in vitro enzymatic assays, and oxonium ion patterns, we propose that the observed O-linked glycans carrying up to five monosaccharides are extended O-GlcNAc's rather than GalNAc-initiated O-glycans.
26280087	4	18	theme	N-linked	823:830	arg1	glycopeptides					832:844	N-linked glycopeptides	823:844	N-linked glycopeptides derived from membrane proteins	823:875	We also report for the first time natural HLA class I presentation of O- and N-linked glycopeptides derived from membrane proteins.
26280087	5	19	theme	located	914:920	arg1	oligosaccharides					922:937	centrally located oligosaccharides	904:937	centrally located oligosaccharides	904:937	HLA class I peptides with centrally located oligosaccharides have been shown to be immunogenic and may thus be important targets for immune surveillance.
26280087	0	20	theme	Extended	0:7	arg1	O-GlcNAc					9:16	Extended O-GlcNAc	0:16	Extended O-GlcNAc on HLA Class-I-Bound Peptides	0:46	Extended O-GlcNAc on HLA Class-I-Bound Peptides.
26280087	3	21	theme	special	553:559	arg1	case					561:564	a special case	551:564	a special case because the loaded HLA class I complex traffics through the endoplasmic reticulum and Golgi apparatus on its way to the cell membrane and is hence exposed to glycosyltransferases	551:743	A cytosolic O-GlcNAc modification is normally terminal and does not extend to produce a polysaccharide, but O-GlcNAc on an HLA peptide presents a special case because the loaded HLA class I complex traffics through the endoplasmic reticulum and Golgi apparatus on its way to the cell membrane and is hence exposed to glycosyltransferases.
26280087	5	22	with	peptides	890:897	arg1	oligosaccharides					922:937	centrally located oligosaccharides	904:937	centrally located oligosaccharides	904:937	HLA class I peptides with centrally located oligosaccharides have been shown to be immunogenic and may thus be important targets for immune surveillance.
26280087	0	23	theme	HLA	21:23	arg1	Peptides					39:46	HLA Class-I-Bound Peptides	21:46	HLA Class-I-Bound Peptides	21:46	Extended O-GlcNAc on HLA Class-I-Bound Peptides.
26280087	4	24	theme	natural	780:786	arg1	presentation					800:811	the first time natural HLA class I presentation	765:811	the first time natural HLA class I presentation of O- and N-linked glycopeptides derived from membrane proteins	765:875	We also report for the first time natural HLA class I presentation of O- and N-linked glycopeptides derived from membrane proteins.
26280087	3	25	theme	loaded	578:583	arg1	complex					597:603	the loaded HLA class I complex	574:603	the loaded HLA class I complex	574:603	A cytosolic O-GlcNAc modification is normally terminal and does not extend to produce a polysaccharide, but O-GlcNAc on an HLA peptide presents a special case because the loaded HLA class I complex traffics through the endoplasmic reticulum and Golgi apparatus on its way to the cell membrane and is hence exposed to glycosyltransferases.
26280087	2	26	theme	O-linked	290:297	arg1	glycans					299:305	the observed O-linked glycans	277:305	the observed O-linked glycans carrying up to five monosaccharides	277:341	Complemented by molecular modeling, in vitro enzymatic assays, and oxonium ion patterns, we propose that the observed O-linked glycans carrying up to five monosaccharides are extended O-GlcNAc's rather than GalNAc-initiated O-glycans.
26280087	4	27	theme	class	792:796	arg1	presentation					800:811	the first time natural HLA class I presentation	765:811	the first time natural HLA class I presentation of O- and N-linked glycopeptides derived from membrane proteins	765:875	We also report for the first time natural HLA class I presentation of O- and N-linked glycopeptides derived from membrane proteins.
26280087	4	28	theme	time	775:778	arg1	presentation					800:811	the first time natural HLA class I presentation	765:811	the first time natural HLA class I presentation of O- and N-linked glycopeptides derived from membrane proteins	765:875	We also report for the first time natural HLA class I presentation of O- and N-linked glycopeptides derived from membrane proteins.
26280087	2	29	theme	molecular	188:196	arg1	modeling					198:205	molecular modeling	188:205	molecular modeling	188:205	Complemented by molecular modeling, in vitro enzymatic assays, and oxonium ion patterns, we propose that the observed O-linked glycans carrying up to five monosaccharides are extended O-GlcNAc's rather than GalNAc-initiated O-glycans.
26280087	2	30	theme	observed	281:288	arg1	glycans					299:305	the observed O-linked glycans	277:305	the observed O-linked glycans carrying up to five monosaccharides	277:341	Complemented by molecular modeling, in vitro enzymatic assays, and oxonium ion patterns, we propose that the observed O-linked glycans carrying up to five monosaccharides are extended O-GlcNAc's rather than GalNAc-initiated O-glycans.
26280087	3	31	theme	cytosolic	409:417	arg1	modification					428:439	A cytosolic O-GlcNAc modification	407:439	A cytosolic O-GlcNAc modification	407:439	A cytosolic O-GlcNAc modification is normally terminal and does not extend to produce a polysaccharide, but O-GlcNAc on an HLA peptide presents a special case because the loaded HLA class I complex traffics through the endoplasmic reticulum and Golgi apparatus on its way to the cell membrane and is hence exposed to glycosyltransferases.
26280087	3	31	theme	cytosolic	409:417	arg1	terminal					453:460	terminal	453:460	terminal	453:460	A cytosolic O-GlcNAc modification is normally terminal and does not extend to produce a polysaccharide, but O-GlcNAc on an HLA peptide presents a special case because the loaded HLA class I complex traffics through the endoplasmic reticulum and Golgi apparatus on its way to the cell membrane and is hence exposed to glycosyltransferases.
26280087	3	32	theme	HLA	585:587	arg1	class					589:593	HLA class I	585:595	the loaded HLA class I complex	574:603	A cytosolic O-GlcNAc modification is normally terminal and does not extend to produce a polysaccharide, but O-GlcNAc on an HLA peptide presents a special case because the loaded HLA class I complex traffics through the endoplasmic reticulum and Golgi apparatus on its way to the cell membrane and is hence exposed to glycosyltransferases.
26280087	0	33	theme	Class-I-Bound	25:37	arg1	Peptides					39:46	HLA Class-I-Bound Peptides	21:46	HLA Class-I-Bound Peptides	21:46	Extended O-GlcNAc on HLA Class-I-Bound Peptides.
26280087	2	34	link	O-linked	290:297	arg1	glycans					299:305	the observed O-linked glycans	277:305	the observed O-linked glycans carrying up to five monosaccharides	277:341	Complemented by molecular modeling, in vitro enzymatic assays, and oxonium ion patterns, we propose that the observed O-linked glycans carrying up to five monosaccharides are extended O-GlcNAc's rather than GalNAc-initiated O-glycans.
26280087	3	35	theme	O-GlcNAc	419:426	arg1	modification					428:439	A cytosolic O-GlcNAc modification	407:439	A cytosolic O-GlcNAc modification	407:439	A cytosolic O-GlcNAc modification is normally terminal and does not extend to produce a polysaccharide, but O-GlcNAc on an HLA peptide presents a special case because the loaded HLA class I complex traffics through the endoplasmic reticulum and Golgi apparatus on its way to the cell membrane and is hence exposed to glycosyltransferases.
26280087	3	35	theme	O-GlcNAc	419:426	arg1	terminal					453:460	terminal	453:460	terminal	453:460	A cytosolic O-GlcNAc modification is normally terminal and does not extend to produce a polysaccharide, but O-GlcNAc on an HLA peptide presents a special case because the loaded HLA class I complex traffics through the endoplasmic reticulum and Golgi apparatus on its way to the cell membrane and is hence exposed to glycosyltransferases.
26280087	3	36	theme	class	589:593	arg1	complex					597:603	the loaded HLA class I complex	574:603	the loaded HLA class I complex	574:603	A cytosolic O-GlcNAc modification is normally terminal and does not extend to produce a polysaccharide, but O-GlcNAc on an HLA peptide presents a special case because the loaded HLA class I complex traffics through the endoplasmic reticulum and Golgi apparatus on its way to the cell membrane and is hence exposed to glycosyltransferases.
26280087	5	37	theme	important	989:997	arg1	targets					999:1005	important targets	989:1005	important targets for immune surveillance	989:1029	HLA class I peptides with centrally located oligosaccharides have been shown to be immunogenic and may thus be important targets for immune surveillance.
26280087	4	38	theme	I	798:798	arg1	presentation					800:811	the first time natural HLA class I presentation	765:811	the first time natural HLA class I presentation of O- and N-linked glycopeptides derived from membrane proteins	765:875	We also report for the first time natural HLA class I presentation of O- and N-linked glycopeptides derived from membrane proteins.
26280087	3	39	theme	endoplasmic	626:636	arg1	reticulum					638:646	the endoplasmic reticulum	622:646	the endoplasmic reticulum	622:646	A cytosolic O-GlcNAc modification is normally terminal and does not extend to produce a polysaccharide, but O-GlcNAc on an HLA peptide presents a special case because the loaded HLA class I complex traffics through the endoplasmic reticulum and Golgi apparatus on its way to the cell membrane and is hence exposed to glycosyltransferases.
26280087	1	40	theme	leukocyte	124:132	arg1	HLA					143:145	HLA	143:145	HLA	143:145	We report unexpected mass spectrometric observations of glycosylated human leukocyte antigen (HLA) class I-bound peptides.
26280087	1	40	theme	leukocyte	124:132	arg1	antigen					134:140	human leukocyte antigen	118:140	glycosylated human leukocyte antigen (HLA) class I-bound peptides	105:169	We report unexpected mass spectrometric observations of glycosylated human leukocyte antigen (HLA) class I-bound peptides.
26280087	5	41	theme	immune	1011:1016	arg1	surveillance					1018:1029	immune surveillance	1011:1029	immune surveillance	1011:1029	HLA class I peptides with centrally located oligosaccharides have been shown to be immunogenic and may thus be important targets for immune surveillance.
26280087	2	42	theme	enzymatic	217:225	arg1	assays					227:232	in vitro enzymatic assays	208:232	in vitro enzymatic assays	208:232	Complemented by molecular modeling, in vitro enzymatic assays, and oxonium ion patterns, we propose that the observed O-linked glycans carrying up to five monosaccharides are extended O-GlcNAc's rather than GalNAc-initiated O-glycans.
26280087	1	43	theme	antigen	134:140	arg1	peptides					162:169	glycosylated human leukocyte antigen (HLA) class I-bound peptides	105:169	glycosylated human leukocyte antigen (HLA) class I-bound peptides	105:169	We report unexpected mass spectrometric observations of glycosylated human leukocyte antigen (HLA) class I-bound peptides.
26280087	2	44	contain	carrying	307:314	arg2	monosaccharides					327:341	up to five monosaccharides	316:341	up to five monosaccharides	316:341	Complemented by molecular modeling, in vitro enzymatic assays, and oxonium ion patterns, we propose that the observed O-linked glycans carrying up to five monosaccharides are extended O-GlcNAc's rather than GalNAc-initiated O-glycans.
26280087	2	44	contain	carrying	307:314	arg1	glycans					299:305	the observed O-linked glycans	277:305	the observed O-linked glycans carrying up to five monosaccharides	277:341	Complemented by molecular modeling, in vitro enzymatic assays, and oxonium ion patterns, we propose that the observed O-linked glycans carrying up to five monosaccharides are extended O-GlcNAc's rather than GalNAc-initiated O-glycans.
26280087	2	45	theme	in	208:209	arg1	assays					227:232	in vitro enzymatic assays	208:232	in vitro enzymatic assays	208:232	Complemented by molecular modeling, in vitro enzymatic assays, and oxonium ion patterns, we propose that the observed O-linked glycans carrying up to five monosaccharides are extended O-GlcNAc's rather than GalNAc-initiated O-glycans.
26280087	4	46	theme	first	769:773	arg1	presentation					800:811	the first time natural HLA class I presentation	765:811	the first time natural HLA class I presentation of O- and N-linked glycopeptides derived from membrane proteins	765:875	We also report for the first time natural HLA class I presentation of O- and N-linked glycopeptides derived from membrane proteins.
26280087	4	47	theme	HLA	788:790	arg1	presentation					800:811	the first time natural HLA class I presentation	765:811	the first time natural HLA class I presentation of O- and N-linked glycopeptides derived from membrane proteins	765:875	We also report for the first time natural HLA class I presentation of O- and N-linked glycopeptides derived from membrane proteins.
26280087	1	48	theme	glycosylated	105:116	arg1	peptides					162:169	glycosylated human leukocyte antigen (HLA) class I-bound peptides	105:169	glycosylated human leukocyte antigen (HLA) class I-bound peptides	105:169	We report unexpected mass spectrometric observations of glycosylated human leukocyte antigen (HLA) class I-bound peptides.
26280087	3	49	theme	HLA	530:532	arg1	peptide					534:540	an HLA peptide	527:540	an HLA peptide	527:540	A cytosolic O-GlcNAc modification is normally terminal and does not extend to produce a polysaccharide, but O-GlcNAc on an HLA peptide presents a special case because the loaded HLA class I complex traffics through the endoplasmic reticulum and Golgi apparatus on its way to the cell membrane and is hence exposed to glycosyltransferases.
26280087	3	50	theme	Golgi	652:656	arg1	apparatus					658:666	Golgi apparatus	652:666	Golgi apparatus	652:666	A cytosolic O-GlcNAc modification is normally terminal and does not extend to produce a polysaccharide, but O-GlcNAc on an HLA peptide presents a special case because the loaded HLA class I complex traffics through the endoplasmic reticulum and Golgi apparatus on its way to the cell membrane and is hence exposed to glycosyltransferases.
26280087	2	51	theme	ion	247:249	arg1	patterns					251:258	oxonium ion patterns	239:258	oxonium ion patterns	239:258	Complemented by molecular modeling, in vitro enzymatic assays, and oxonium ion patterns, we propose that the observed O-linked glycans carrying up to five monosaccharides are extended O-GlcNAc's rather than GalNAc-initiated O-glycans.
26280087	4	52	attach	derived	846:852	arg1	proteins					868:875	membrane proteins	859:875	membrane proteins	859:875	We also report for the first time natural HLA class I presentation of O- and N-linked glycopeptides derived from membrane proteins.
26280087	4	52	attach	derived	846:852	arg2	glycopeptides					832:844	N-linked glycopeptides	823:844	N-linked glycopeptides derived from membrane proteins	823:875	We also report for the first time natural HLA class I presentation of O- and N-linked glycopeptides derived from membrane proteins.
26280087	2	53	theme	oxonium	239:245	arg1	patterns					251:258	oxonium ion patterns	239:258	oxonium ion patterns	239:258	Complemented by molecular modeling, in vitro enzymatic assays, and oxonium ion patterns, we propose that the observed O-linked glycans carrying up to five monosaccharides are extended O-GlcNAc's rather than GalNAc-initiated O-glycans.
26280087	1	54	theme	class	148:152	arg1	peptides					162:169	glycosylated human leukocyte antigen (HLA) class I-bound peptides	105:169	glycosylated human leukocyte antigen (HLA) class I-bound peptides	105:169	We report unexpected mass spectrometric observations of glycosylated human leukocyte antigen (HLA) class I-bound peptides.
26280087	2	55	dep	five	322:325	arg1	to					319:320	to	319:320	to	319:320	Complemented by molecular modeling, in vitro enzymatic assays, and oxonium ion patterns, we propose that the observed O-linked glycans carrying up to five monosaccharides are extended O-GlcNAc's rather than GalNAc-initiated O-glycans.
26280087	2	56	dep	in	208:209	arg1	vitro					211:215	vitro	211:215	vitro	211:215	Complemented by molecular modeling, in vitro enzymatic assays, and oxonium ion patterns, we propose that the observed O-linked glycans carrying up to five monosaccharides are extended O-GlcNAc's rather than GalNAc-initiated O-glycans.
24373136	0	0	theme	post-vaccination	73:88	arg1	serotypes					99:107	post-vaccination emerging serotypes	73:107	post-vaccination emerging serotypes colonizing the human nasopharynx	73:140	In vitro biofilm formation by Streptococcus pneumoniae as a predictor of post-vaccination emerging serotypes colonizing the human nasopharynx.
24373136	11	1	dep	in	1471:1472	arg1	vitro					1474:1478	vitro	1474:1478	vitro	1474:1478	Testing of the in vitro biofilm-forming ability of isogenic transformants expressing different capsular polysaccharides may help predict the emergence of colonizing, non-vaccine serotypes.
24373136	9	2	theme	biofilm	1273:1279	arg1	formation					1281:1289	impaired biofilm formation	1264:1289	impaired biofilm formation	1264:1289	However, the strains of serogroup 18 showed impaired biofilm formation.
24373136	11	3	theme	isogenic	1507:1514	arg1	transformants					1516:1528	isogenic transformants	1507:1528	isogenic transformants expressing different capsular polysaccharides	1507:1574	Testing of the in vitro biofilm-forming ability of isogenic transformants expressing different capsular polysaccharides may help predict the emergence of colonizing, non-vaccine serotypes.
24373136	1	4	theme	serotypes	257:265	arg1	rise					237:240	the rise	233:240	the rise of non-vaccine serotypes colonizing the human nasopharynx	233:298	The increasing use of the 7-valent pneumococcal conjugate vaccine has been accompanied by the rise of non-vaccine serotypes colonizing the human nasopharynx.
24373136	8	5	theme	capsular	1119:1126	arg1	disaccharides					1128:1140	very similar capsular disaccharides	1106:1140	very similar capsular disaccharides	1106:1140	Serotype 18A and serotypes 18B/18C have very similar capsular disaccharides: α-D-GlcpNAc-(1→3)-β-L-Rhap-(1→ and α-D-Glcp-(1→3)-β-L-Rhap-(1→ respectively.
24373136	10	6	theme	capsular	1362:1369	arg1	polysaccharide					1371:1384	the capsular polysaccharide	1358:1384	the capsular polysaccharide	1358:1384	These results indicate that the chemical composition/structure of the capsular polysaccharide is crucial to the biofilm-forming capacity of pneumococcal serotypes.
24373136	11	7	theme	biofilm-forming	1480:1494	arg1	ability					1496:1502	the in vitro biofilm-forming ability	1467:1502	the in vitro biofilm-forming ability of isogenic transformants expressing different capsular polysaccharides	1467:1574	Testing of the in vitro biofilm-forming ability of isogenic transformants expressing different capsular polysaccharides may help predict the emergence of colonizing, non-vaccine serotypes.
24373136	4	8	theme	isolates	599:606	arg1	capacity					565:572	The biofilm-forming capacity	545:572	The biofilm-forming capacity of pneumococcal clinical isolates with different types of capsular polysaccharide and various isogenic transformants	545:689	The biofilm-forming capacity of pneumococcal clinical isolates with different types of capsular polysaccharide and various isogenic transformants was examined.
24373136	0	9	theme	serotypes	99:107	arg1	predictor					60:68	a predictor	58:68	a predictor of post-vaccination emerging serotypes colonizing the human nasopharynx	58:140	In vitro biofilm formation by Streptococcus pneumoniae as a predictor of post-vaccination emerging serotypes colonizing the human nasopharynx.
24373136	4	10	theme	polysaccharide	641:654	arg1	types					623:627	different types	613:627	different types of capsular polysaccharide and various isogenic transformants	613:689	The biofilm-forming capacity of pneumococcal clinical isolates with different types of capsular polysaccharide and various isogenic transformants was examined.
24373136	10	11	theme	biofilm-forming	1404:1418	arg1	capacity					1420:1427	the biofilm-forming capacity	1400:1427	the biofilm-forming capacity of pneumococcal serotypes	1400:1453	These results indicate that the chemical composition/structure of the capsular polysaccharide is crucial to the biofilm-forming capacity of pneumococcal serotypes.
24373136	11	12	theme	capsular	1551:1558	arg1	polysaccharides					1560:1574	different capsular polysaccharides	1541:1574	different capsular polysaccharides	1541:1574	Testing of the in vitro biofilm-forming ability of isogenic transformants expressing different capsular polysaccharides may help predict the emergence of colonizing, non-vaccine serotypes.
24373136	2	13	theme	vast	305:308	arg1	majority					310:317	The vast majority	301:317	The vast majority of infections	301:331	The vast majority of infections are caused by microorganisms that grow in biofilms.
24373136	11	14	theme	ability	1496:1502	arg1	Testing					1456:1462	Testing	1456:1462	Testing of the in vitro biofilm-forming ability of isogenic transformants expressing different capsular polysaccharides	1456:1574	Testing of the in vitro biofilm-forming ability of isogenic transformants expressing different capsular polysaccharides may help predict the emergence of colonizing, non-vaccine serotypes.
24373136	0	15	theme	emerging	90:97	arg1	serotypes					99:107	post-vaccination emerging serotypes	73:107	post-vaccination emerging serotypes colonizing the human nasopharynx	73:140	In vitro biofilm formation by Streptococcus pneumoniae as a predictor of post-vaccination emerging serotypes colonizing the human nasopharynx.
24373136	11	16	theme	colonizing	1610:1619	arg1	serotypes					1634:1642	colonizing, non-vaccine serotypes	1610:1642	colonizing, non-vaccine serotypes	1610:1642	Testing of the in vitro biofilm-forming ability of isogenic transformants expressing different capsular polysaccharides may help predict the emergence of colonizing, non-vaccine serotypes.
24373136	11	17	theme	transformants	1516:1528	arg1	ability					1496:1502	the in vitro biofilm-forming ability	1467:1502	the in vitro biofilm-forming ability of isogenic transformants expressing different capsular polysaccharides	1467:1574	Testing of the in vitro biofilm-forming ability of isogenic transformants expressing different capsular polysaccharides may help predict the emergence of colonizing, non-vaccine serotypes.
24373136	1	18	theme	human	282:286	arg1	nasopharynx					288:298	the human nasopharynx	278:298	the human nasopharynx	278:298	The increasing use of the 7-valent pneumococcal conjugate vaccine has been accompanied by the rise of non-vaccine serotypes colonizing the human nasopharynx.
24373136	3	19	theme	polysaccharide	529:542	arg1	presence					508:515	the presence	504:515	the presence of capsular polysaccharide	504:542	It has recently been shown that the formation of Streptococcus pneumoniae biofilms in vivo and in vitro is hindered by the presence of capsular polysaccharide.
24373136	1	20	theme	7-valent	169:176	arg1	vaccine					201:207	the 7-valent pneumococcal conjugate vaccine	165:207	the 7-valent pneumococcal conjugate vaccine	165:207	The increasing use of the 7-valent pneumococcal conjugate vaccine has been accompanied by the rise of non-vaccine serotypes colonizing the human nasopharynx.
24373136	4	21	theme	different	613:621	arg1	types					623:627	different types	613:627	different types of capsular polysaccharide and various isogenic transformants	613:689	The biofilm-forming capacity of pneumococcal clinical isolates with different types of capsular polysaccharide and various isogenic transformants was examined.
24373136	6	22	theme	biofilm-forming	898:912	arg1	capacity					914:921	significant biofilm-forming capacity	886:921	significant biofilm-forming capacity	886:921	Strains of serogroup 6 also showed significant biofilm-forming capacity.
24373136	5	23	theme	quantity	780:787	arg1	%					771:771	≥ 80%	767:771	≥ 80% of the quantity of biofilm associated with a non-encapsulated control strain	767:848	Strains of serotypes 19A and 19F, but not 19B and 19C, formed ≥ 80% of the quantity of biofilm associated with a non-encapsulated control strain.
24373136	5	23	theme	quantity	780:787	arg1	quantity					780:787	the quantity	776:787	the quantity of biofilm associated with a non-encapsulated control strain	776:848	Strains of serotypes 19A and 19F, but not 19B and 19C, formed ≥ 80% of the quantity of biofilm associated with a non-encapsulated control strain.
24373136	1	24	theme	pneumococcal	178:189	arg1	vaccine					201:207	the 7-valent pneumococcal conjugate vaccine	165:207	the 7-valent pneumococcal conjugate vaccine	165:207	The increasing use of the 7-valent pneumococcal conjugate vaccine has been accompanied by the rise of non-vaccine serotypes colonizing the human nasopharynx.
24373136	8	25	dep	have	1101:1104	arg1	1→					1171:1172	1→	1171:1172	1→	1171:1172	Serotype 18A and serotypes 18B/18C have very similar capsular disaccharides: α-D-GlcpNAc-(1→3)-β-L-Rhap-(1→ and α-D-Glcp-(1→3)-β-L-Rhap-(1→ respectively.
24373136	8	25	dep	have	1101:1104	arg1	1→					1203:1204	1→	1203:1204	1→	1203:1204	Serotype 18A and serotypes 18B/18C have very similar capsular disaccharides: α-D-GlcpNAc-(1→3)-β-L-Rhap-(1→ and α-D-Glcp-(1→3)-β-L-Rhap-(1→ respectively.
24373136	6	26	theme	significant	886:896	arg1	capacity					914:921	significant biofilm-forming capacity	886:921	significant biofilm-forming capacity	886:921	Strains of serogroup 6 also showed significant biofilm-forming capacity.
24373136	10	27	theme	serotypes	1445:1453	arg1	capacity					1420:1427	the biofilm-forming capacity	1400:1427	the biofilm-forming capacity of pneumococcal serotypes	1400:1453	These results indicate that the chemical composition/structure of the capsular polysaccharide is crucial to the biofilm-forming capacity of pneumococcal serotypes.
24373136	11	28	dep	colonizing	1610:1619	arg1	non-vaccine					1622:1632	non-vaccine	1622:1632	non-vaccine	1622:1632	Testing of the in vitro biofilm-forming ability of isogenic transformants expressing different capsular polysaccharides may help predict the emergence of colonizing, non-vaccine serotypes.
24373136	1	29	theme	conjugate	191:199	arg1	vaccine					201:207	the 7-valent pneumococcal conjugate vaccine	165:207	the 7-valent pneumococcal conjugate vaccine	165:207	The increasing use of the 7-valent pneumococcal conjugate vaccine has been accompanied by the rise of non-vaccine serotypes colonizing the human nasopharynx.
24373136	0	30	theme	In	0:1	arg1	formation					17:25	In vitro biofilm formation	0:25	In vitro biofilm formation by Streptococcus pneumoniae as a predictor of post-vaccination emerging serotypes colonizing the human nasopharynx.	0:141	In vitro biofilm formation by Streptococcus pneumoniae as a predictor of post-vaccination emerging serotypes colonizing the human nasopharynx.
24373136	10	31	theme	pneumococcal	1432:1443	arg1	serotypes					1445:1453	pneumococcal serotypes	1432:1453	pneumococcal serotypes	1432:1453	These results indicate that the chemical composition/structure of the capsular polysaccharide is crucial to the biofilm-forming capacity of pneumococcal serotypes.
24373136	2	32	theme	infections	322:331	arg1	majority					310:317	The vast majority	301:317	The vast majority of infections	301:331	The vast majority of infections are caused by microorganisms that grow in biofilms.
24373136	1	33	theme	vaccine	201:207	arg1	use					158:160	The increasing use	143:160	The increasing use of the 7-valent pneumococcal conjugate vaccine	143:207	The increasing use of the 7-valent pneumococcal conjugate vaccine has been accompanied by the rise of non-vaccine serotypes colonizing the human nasopharynx.
24373136	5	34	theme	non-encapsulated	818:833	arg1	strain					843:848	a non-encapsulated control strain	816:848	a non-encapsulated control strain	816:848	Strains of serotypes 19A and 19F, but not 19B and 19C, formed ≥ 80% of the quantity of biofilm associated with a non-encapsulated control strain.
24373136	10	35	theme	polysaccharide	1371:1384	arg1	composition/structure					1333:1353	the chemical composition/structure	1320:1353	the chemical composition/structure of the capsular polysaccharide	1320:1384	These results indicate that the chemical composition/structure of the capsular polysaccharide is crucial to the biofilm-forming capacity of pneumococcal serotypes.
24373136	10	35	theme	polysaccharide	1371:1384	arg1	crucial					1389:1395	crucial	1389:1395	crucial	1389:1395	These results indicate that the chemical composition/structure of the capsular polysaccharide is crucial to the biofilm-forming capacity of pneumococcal serotypes.
24373136	11	36	theme	different	1541:1549	arg1	polysaccharides					1560:1574	different capsular polysaccharides	1541:1574	different capsular polysaccharides	1541:1574	Testing of the in vitro biofilm-forming ability of isogenic transformants expressing different capsular polysaccharides may help predict the emergence of colonizing, non-vaccine serotypes.
24373136	4	37	theme	isogenic	668:675	arg1	transformants					677:689	various isogenic transformants	660:689	various isogenic transformants	660:689	The biofilm-forming capacity of pneumococcal clinical isolates with different types of capsular polysaccharide and various isogenic transformants was examined.
24373136	0	38	theme	biofilm	9:15	arg1	formation					17:25	In vitro biofilm formation	0:25	In vitro biofilm formation by Streptococcus pneumoniae as a predictor of post-vaccination emerging serotypes colonizing the human nasopharynx.	0:141	In vitro biofilm formation by Streptococcus pneumoniae as a predictor of post-vaccination emerging serotypes colonizing the human nasopharynx.
24373136	4	39	theme	clinical	590:597	arg1	isolates					599:606	pneumococcal clinical isolates	577:606	pneumococcal clinical isolates with different types of capsular polysaccharide and various isogenic transformants	577:689	The biofilm-forming capacity of pneumococcal clinical isolates with different types of capsular polysaccharide and various isogenic transformants was examined.
24373136	0	40	theme	human	124:128	arg1	nasopharynx					130:140	the human nasopharynx	120:140	the human nasopharynx	120:140	In vitro biofilm formation by Streptococcus pneumoniae as a predictor of post-vaccination emerging serotypes colonizing the human nasopharynx.
24373136	4	41	theme	various	660:666	arg1	transformants					677:689	various isogenic transformants	660:689	various isogenic transformants	660:689	The biofilm-forming capacity of pneumococcal clinical isolates with different types of capsular polysaccharide and various isogenic transformants was examined.
24373136	0	42	theme	Streptococcus	30:42	arg1	pneumoniae					44:53	Streptococcus pneumoniae	30:53	Streptococcus pneumoniae	30:53	In vitro biofilm formation by Streptococcus pneumoniae as a predictor of post-vaccination emerging serotypes colonizing the human nasopharynx.
24373136	4	43	theme	pneumococcal	577:588	arg1	isolates					599:606	pneumococcal clinical isolates	577:606	pneumococcal clinical isolates with different types of capsular polysaccharide and various isogenic transformants	577:689	The biofilm-forming capacity of pneumococcal clinical isolates with different types of capsular polysaccharide and various isogenic transformants was examined.
24373136	11	44	theme	serotypes	1634:1642	arg1	emergence					1597:1605	the emergence	1593:1605	the emergence of colonizing, non-vaccine serotypes	1593:1642	Testing of the in vitro biofilm-forming ability of isogenic transformants expressing different capsular polysaccharides may help predict the emergence of colonizing, non-vaccine serotypes.
24373136	7	45	dep	contain	979:985	arg1	1→					1030:1031	1→	1030:1031	1→	1030:1031	The capsules of serotypes 19A and 19F, and serogroup 6 contain the disaccharides α-D-Glcp-(1→2)-α-L-Rhap-(1→ and α-D-Glcp-(1→3)-α-L-Rhap-(1→.
24373136	7	45	dep	contain	979:985	arg1	1→					1062:1063	1→	1062:1063	1→	1062:1063	The capsules of serotypes 19A and 19F, and serogroup 6 contain the disaccharides α-D-Glcp-(1→2)-α-L-Rhap-(1→ and α-D-Glcp-(1→3)-α-L-Rhap-(1→.
24373136	3	46	theme	pneumoniae	448:457	arg1	biofilms					459:466	Streptococcus pneumoniae biofilms	434:466	Streptococcus pneumoniae biofilms	434:466	It has recently been shown that the formation of Streptococcus pneumoniae biofilms in vivo and in vitro is hindered by the presence of capsular polysaccharide.
24373136	8	47	theme	Serotype	1066:1073	arg1	18A					1075:1077	Serotype 18A	1066:1077	Serotype 18A	1066:1077	Serotype 18A and serotypes 18B/18C have very similar capsular disaccharides: α-D-GlcpNAc-(1→3)-β-L-Rhap-(1→ and α-D-Glcp-(1→3)-β-L-Rhap-(1→ respectively.
24373136	7	48	theme	-α-L-Rhap-	1019:1028	arg1	disaccharides					991:1003	the disaccharides	987:1003	the disaccharides α-D-Glcp-(1→2)-α-L-Rhap-	987:1028	The capsules of serotypes 19A and 19F, and serogroup 6 contain the disaccharides α-D-Glcp-(1→2)-α-L-Rhap-(1→ and α-D-Glcp-(1→3)-α-L-Rhap-(1→.
24373136	4	49	theme	transformants	677:689	arg1	types					623:627	different types	613:627	different types of capsular polysaccharide and various isogenic transformants	613:689	The biofilm-forming capacity of pneumococcal clinical isolates with different types of capsular polysaccharide and various isogenic transformants was examined.
24373136	5	50	theme	biofilm	792:798	arg1	quantity					780:787	the quantity	776:787	the quantity of biofilm associated with a non-encapsulated control strain	776:848	Strains of serotypes 19A and 19F, but not 19B and 19C, formed ≥ 80% of the quantity of biofilm associated with a non-encapsulated control strain.
24373136	5	51	theme	serotypes	716:724	arg1	Strains					705:711	Strains	705:711	Strains of serotypes 19A and 19F	705:736	Strains of serotypes 19A and 19F, but not 19B and 19C, formed ≥ 80% of the quantity of biofilm associated with a non-encapsulated control strain.
24373136	6	52	theme	serogroup	862:870	arg1	Strains					851:857	Strains	851:857	Strains of serogroup 6	851:872	Strains of serogroup 6 also showed significant biofilm-forming capacity.
24373136	9	53	theme	impaired	1264:1271	arg1	formation					1281:1289	impaired biofilm formation	1264:1289	impaired biofilm formation	1264:1289	However, the strains of serogroup 18 showed impaired biofilm formation.
24373136	9	54	theme	serogroup	1244:1252	arg1	strains					1233:1239	the strains	1229:1239	the strains of serogroup 18	1229:1255	However, the strains of serogroup 18 showed impaired biofilm formation.
24373136	4	55	theme	biofilm-forming	549:563	arg1	capacity					565:572	The biofilm-forming capacity	545:572	The biofilm-forming capacity of pneumococcal clinical isolates with different types of capsular polysaccharide and various isogenic transformants	545:689	The biofilm-forming capacity of pneumococcal clinical isolates with different types of capsular polysaccharide and various isogenic transformants was examined.
24373136	8	56	dep	18A	1075:1077	arg1	18B/18C					1093:1099	18B/18C	1093:1099	18B/18C	1093:1099	Serotype 18A and serotypes 18B/18C have very similar capsular disaccharides: α-D-GlcpNAc-(1→3)-β-L-Rhap-(1→ and α-D-Glcp-(1→3)-β-L-Rhap-(1→ respectively.
24373136	8	57	contain	have	1101:1104	arg1	serotypes					1083:1091	serotypes	1083:1091	serotypes	1083:1091	Serotype 18A and serotypes 18B/18C have very similar capsular disaccharides: α-D-GlcpNAc-(1→3)-β-L-Rhap-(1→ and α-D-Glcp-(1→3)-β-L-Rhap-(1→ respectively.
24373136	8	57	contain	have	1101:1104	arg1	18A					1075:1077	Serotype 18A	1066:1077	Serotype 18A	1066:1077	Serotype 18A and serotypes 18B/18C have very similar capsular disaccharides: α-D-GlcpNAc-(1→3)-β-L-Rhap-(1→ and α-D-Glcp-(1→3)-β-L-Rhap-(1→ respectively.
24373136	8	57	contain	have	1101:1104	arg2	disaccharides					1128:1140	very similar capsular disaccharides	1106:1140	very similar capsular disaccharides	1106:1140	Serotype 18A and serotypes 18B/18C have very similar capsular disaccharides: α-D-GlcpNAc-(1→3)-β-L-Rhap-(1→ and α-D-Glcp-(1→3)-β-L-Rhap-(1→ respectively.
24373136	11	58	theme	in	1471:1472	arg1	ability					1496:1502	the in vitro biofilm-forming ability	1467:1502	the in vitro biofilm-forming ability of isogenic transformants expressing different capsular polysaccharides	1467:1574	Testing of the in vitro biofilm-forming ability of isogenic transformants expressing different capsular polysaccharides may help predict the emergence of colonizing, non-vaccine serotypes.
24373136	4	59	with	isolates	599:606	arg1	types					623:627	different types	613:627	different types of capsular polysaccharide and various isogenic transformants	613:689	The biofilm-forming capacity of pneumococcal clinical isolates with different types of capsular polysaccharide and various isogenic transformants was examined.
24373136	7	60	theme	serotypes	940:948	arg1	capsules					928:935	The capsules	924:935	The capsules of serotypes 19A and 19F, and serogroup 6	924:977	The capsules of serotypes 19A and 19F, and serogroup 6 contain the disaccharides α-D-Glcp-(1→2)-α-L-Rhap-(1→ and α-D-Glcp-(1→3)-α-L-Rhap-(1→.
24373136	4	61	theme	capsular	632:639	arg1	polysaccharide					641:654	capsular polysaccharide	632:654	capsular polysaccharide	632:654	The biofilm-forming capacity of pneumococcal clinical isolates with different types of capsular polysaccharide and various isogenic transformants was examined.
24373136	5	62	theme	19F	734:736	arg1	Strains					705:711	Strains	705:711	Strains of serotypes 19A and 19F	705:736	Strains of serotypes 19A and 19F, but not 19B and 19C, formed ≥ 80% of the quantity of biofilm associated with a non-encapsulated control strain.
24373136	5	63	theme	control	835:841	arg1	strain					843:848	a non-encapsulated control strain	816:848	a non-encapsulated control strain	816:848	Strains of serotypes 19A and 19F, but not 19B and 19C, formed ≥ 80% of the quantity of biofilm associated with a non-encapsulated control strain.
24373136	10	64	theme	chemical	1324:1331	arg1	composition/structure					1333:1353	the chemical composition/structure	1320:1353	the chemical composition/structure of the capsular polysaccharide	1320:1384	These results indicate that the chemical composition/structure of the capsular polysaccharide is crucial to the biofilm-forming capacity of pneumococcal serotypes.
24373136	10	64	theme	chemical	1324:1331	arg1	crucial					1389:1395	crucial	1389:1395	crucial	1389:1395	These results indicate that the chemical composition/structure of the capsular polysaccharide is crucial to the biofilm-forming capacity of pneumococcal serotypes.
24373136	8	65	theme	similar	1111:1117	arg1	disaccharides					1128:1140	very similar capsular disaccharides	1106:1140	very similar capsular disaccharides	1106:1140	Serotype 18A and serotypes 18B/18C have very similar capsular disaccharides: α-D-GlcpNAc-(1→3)-β-L-Rhap-(1→ and α-D-Glcp-(1→3)-β-L-Rhap-(1→ respectively.
24373136	1	66	theme	increasing	147:156	arg1	use					158:160	The increasing use	143:160	The increasing use of the 7-valent pneumococcal conjugate vaccine	143:207	The increasing use of the 7-valent pneumococcal conjugate vaccine has been accompanied by the rise of non-vaccine serotypes colonizing the human nasopharynx.
24373136	3	67	theme	biofilms	459:466	arg1	formation					421:429	the formation	417:429	the formation of Streptococcus pneumoniae biofilms in vivo and in vitro	417:487	It has recently been shown that the formation of Streptococcus pneumoniae biofilms in vivo and in vitro is hindered by the presence of capsular polysaccharide.
24373136	7	68	contain	contain	979:985	arg1	capsules					928:935	The capsules	924:935	The capsules of serotypes 19A and 19F, and serogroup 6	924:977	The capsules of serotypes 19A and 19F, and serogroup 6 contain the disaccharides α-D-Glcp-(1→2)-α-L-Rhap-(1→ and α-D-Glcp-(1→3)-α-L-Rhap-(1→.
24373136	7	68	contain	contain	979:985	arg2	disaccharides					991:1003	the disaccharides	987:1003	the disaccharides α-D-Glcp-(1→2)-α-L-Rhap-	987:1028	The capsules of serotypes 19A and 19F, and serogroup 6 contain the disaccharides α-D-Glcp-(1→2)-α-L-Rhap-(1→ and α-D-Glcp-(1→3)-α-L-Rhap-(1→.
24373136	0	69	dep	In	0:1	arg1	vitro					3:7	vitro	3:7	vitro	3:7	In vitro biofilm formation by Streptococcus pneumoniae as a predictor of post-vaccination emerging serotypes colonizing the human nasopharynx.
24373136	3	70	theme	capsular	520:527	arg1	polysaccharide					529:542	capsular polysaccharide	520:542	capsular polysaccharide	520:542	It has recently been shown that the formation of Streptococcus pneumoniae biofilms in vivo and in vitro is hindered by the presence of capsular polysaccharide.
24373136	1	71	theme	non-vaccine	245:255	arg1	serotypes					257:265	non-vaccine serotypes	245:265	non-vaccine serotypes colonizing the human nasopharynx	245:298	The increasing use of the 7-valent pneumococcal conjugate vaccine has been accompanied by the rise of non-vaccine serotypes colonizing the human nasopharynx.
27110762	4	0	from	influence	552:560	arg1	release					641:647	the release	637:647	the release of aroma compounds	637:666	The results showed varying degrees of influence of sucrose fatty acid ester, soybean lecithin, Tween 80 and xanthan gum on the release of aroma compounds.
27110762	5	1	theme	aroma	689:693	arg1	compounds					695:703	other aroma compounds	683:703	other aroma compounds	683:703	Compared with other aroma compounds, ethyl acetate was more likely to be conserved in the solution system, while the amount of limonene released was the highest among these seven aroma compounds.
27110762	4	2	theme	gum	630:632	arg1	influence					552:560	influence	552:560	influence of sucrose fatty acid ester, soybean lecithin, Tween 80 and xanthan gum on the release of aroma compounds	552:666	The results showed varying degrees of influence of sucrose fatty acid ester, soybean lecithin, Tween 80 and xanthan gum on the release of aroma compounds.
27110762	3	3	from	effects	398:404	arg1	release					471:477	aroma release	465:477	aroma release	465:477	The effects of the emulsifier systems supplemented with xanthan gum on aroma release were also studied in the same way.
27110762	2	4	theme	Tween	323:327	arg1	concentrations					266:279	varying concentrations	258:279	varying concentrations of xanthan gum, sucrose fatty acid ester, Tween 80 and soybean lecithin	258:351	Solid-phase microextraction (SPME) and GC-MS were used to study the effects of varying concentrations of xanthan gum, sucrose fatty acid ester, Tween 80 and soybean lecithin on the release of seven aroma compounds.
27110762	7	5	theme	significant	1154:1164	arg1	effects					1166:1172	significant effects	1154:1172	significant effects	1154:1172	The present studies showed that the composition and structure of emulsifiers and specific interactions between emulsifiers and aroma molecules have significant effects on aroma release.
27110762	5	6	theme	limonene	796:803	arg1	limonene					796:803	limonene	796:803	limonene	796:803	Compared with other aroma compounds, ethyl acetate was more likely to be conserved in the solution system, while the amount of limonene released was the highest among these seven aroma compounds.
27110762	5	6	theme	limonene	796:803	arg1	amount					786:791	the amount	782:791	the amount of limonene released	782:812	Compared with other aroma compounds, ethyl acetate was more likely to be conserved in the solution system, while the amount of limonene released was the highest among these seven aroma compounds.
27110762	5	6	theme	limonene	796:803	arg1	highest					822:828	highest	822:828	highest	822:828	Compared with other aroma compounds, ethyl acetate was more likely to be conserved in the solution system, while the amount of limonene released was the highest among these seven aroma compounds.
27110762	3	7	theme	aroma	465:469	arg1	release					471:477	aroma release	465:477	aroma release	465:477	The effects of the emulsifier systems supplemented with xanthan gum on aroma release were also studied in the same way.
27110762	6	8	theme	different	880:888	arg1	emulsifiers					890:900	different emulsifiers	880:900	different emulsifiers	880:900	In conclusion, different emulsifiers and complexes showed different surface properties that tend to interact with different aroma molecules.
27110762	4	9	from	degrees	541:547	arg1	release					641:647	the release	637:647	the release of aroma compounds	637:666	The results showed varying degrees of influence of sucrose fatty acid ester, soybean lecithin, Tween 80 and xanthan gum on the release of aroma compounds.
27110762	2	10	theme	concentrations	266:279	arg1	effects					247:253	the effects	243:253	the effects of varying concentrations of xanthan gum, sucrose fatty acid ester, Tween 80 and soybean lecithin on the release of seven aroma compounds	243:391	Solid-phase microextraction (SPME) and GC-MS were used to study the effects of varying concentrations of xanthan gum, sucrose fatty acid ester, Tween 80 and soybean lecithin on the release of seven aroma compounds.
27110762	3	11	theme	same	504:507	arg1	way					509:511	the same way	500:511	the same way	500:511	The effects of the emulsifier systems supplemented with xanthan gum on aroma release were also studied in the same way.
27110762	2	12	theme	varying	258:264	arg1	concentrations					266:279	varying concentrations	258:279	varying concentrations of xanthan gum, sucrose fatty acid ester, Tween 80 and soybean lecithin	258:351	Solid-phase microextraction (SPME) and GC-MS were used to study the effects of varying concentrations of xanthan gum, sucrose fatty acid ester, Tween 80 and soybean lecithin on the release of seven aroma compounds.
27110762	2	13	used	used	229:232	arg2	SPME					208:211	SPME	208:211	SPME	208:211	Solid-phase microextraction (SPME) and GC-MS were used to study the effects of varying concentrations of xanthan gum, sucrose fatty acid ester, Tween 80 and soybean lecithin on the release of seven aroma compounds.
27110762	2	13	used	used	229:232	arg2	microextraction					191:205	Solid-phase microextraction	179:205	Solid-phase microextraction (SPME)	179:212	Solid-phase microextraction (SPME) and GC-MS were used to study the effects of varying concentrations of xanthan gum, sucrose fatty acid ester, Tween 80 and soybean lecithin on the release of seven aroma compounds.
27110762	2	13	used	used	229:232	arg2	GC-MS					218:222	GC-MS	218:222	GC-MS	218:222	Solid-phase microextraction (SPME) and GC-MS were used to study the effects of varying concentrations of xanthan gum, sucrose fatty acid ester, Tween 80 and soybean lecithin on the release of seven aroma compounds.
27110762	2	14	theme	sucrose	297:303	arg1	ester					316:320	sucrose fatty acid ester	297:320	sucrose fatty acid ester	297:320	Solid-phase microextraction (SPME) and GC-MS were used to study the effects of varying concentrations of xanthan gum, sucrose fatty acid ester, Tween 80 and soybean lecithin on the release of seven aroma compounds.
27110762	1	15	theme	different	92:100	arg1	emulsifiers					102:112	different emulsifiers	92:112	different emulsifiers	92:112	This study aimed to determine the influence of different emulsifiers or xanthan-emulsifier systems on the release of aroma compounds.
27110762	7	16	theme	present	1010:1016	arg1	studies					1018:1024	The present studies	1006:1024	The present studies	1006:1024	The present studies showed that the composition and structure of emulsifiers and specific interactions between emulsifiers and aroma molecules have significant effects on aroma release.
27110762	4	17	theme	fatty	573:577	arg1	ester					584:588	sucrose fatty acid ester	565:588	sucrose fatty acid ester	565:588	The results showed varying degrees of influence of sucrose fatty acid ester, soybean lecithin, Tween 80 and xanthan gum on the release of aroma compounds.
27110762	2	18	theme	lecithin	344:351	arg1	concentrations					266:279	varying concentrations	258:279	varying concentrations of xanthan gum, sucrose fatty acid ester, Tween 80 and soybean lecithin	258:351	Solid-phase microextraction (SPME) and GC-MS were used to study the effects of varying concentrations of xanthan gum, sucrose fatty acid ester, Tween 80 and soybean lecithin on the release of seven aroma compounds.
27110762	4	19	theme	lecithin	599:606	arg1	influence					552:560	influence	552:560	influence of sucrose fatty acid ester, soybean lecithin, Tween 80 and xanthan gum on the release of aroma compounds	552:666	The results showed varying degrees of influence of sucrose fatty acid ester, soybean lecithin, Tween 80 and xanthan gum on the release of aroma compounds.
27110762	4	20	theme	varying	533:539	arg1	degrees					541:547	varying degrees	533:547	varying degrees of influence of sucrose fatty acid ester, soybean lecithin, Tween 80 and xanthan gum on the release of aroma compounds	533:666	The results showed varying degrees of influence of sucrose fatty acid ester, soybean lecithin, Tween 80 and xanthan gum on the release of aroma compounds.
27110762	4	21	theme	acid	579:582	arg1	ester					584:588	sucrose fatty acid ester	565:588	sucrose fatty acid ester	565:588	The results showed varying degrees of influence of sucrose fatty acid ester, soybean lecithin, Tween 80 and xanthan gum on the release of aroma compounds.
27110762	7	22	theme	aroma	1133:1137	arg1	molecules					1139:1147	aroma molecules	1133:1147	aroma molecules	1133:1147	The present studies showed that the composition and structure of emulsifiers and specific interactions between emulsifiers and aroma molecules have significant effects on aroma release.
27110762	3	23	theme	xanthan	450:456	arg1	gum					458:460	xanthan gum	450:460	xanthan gum	450:460	The effects of the emulsifier systems supplemented with xanthan gum on aroma release were also studied in the same way.
27110762	1	24	theme	aroma	162:166	arg1	compounds					168:176	aroma compounds	162:176	aroma compounds	162:176	This study aimed to determine the influence of different emulsifiers or xanthan-emulsifier systems on the release of aroma compounds.
27110762	4	25	theme	compounds	658:666	arg1	release					641:647	the release	637:647	the release of aroma compounds	637:666	The results showed varying degrees of influence of sucrose fatty acid ester, soybean lecithin, Tween 80 and xanthan gum on the release of aroma compounds.
27110762	6	26	theme	different	979:987	arg1	molecules					995:1003	different aroma molecules	979:1003	different aroma molecules	979:1003	In conclusion, different emulsifiers and complexes showed different surface properties that tend to interact with different aroma molecules.
27110762	1	27	theme	compounds	168:176	arg1	release					151:157	the release	147:157	the release of aroma compounds	147:176	This study aimed to determine the influence of different emulsifiers or xanthan-emulsifier systems on the release of aroma compounds.
27110762	2	28	theme	gum	292:294	arg1	concentrations					266:279	varying concentrations	258:279	varying concentrations of xanthan gum, sucrose fatty acid ester, Tween 80 and soybean lecithin	258:351	Solid-phase microextraction (SPME) and GC-MS were used to study the effects of varying concentrations of xanthan gum, sucrose fatty acid ester, Tween 80 and soybean lecithin on the release of seven aroma compounds.
27110762	2	29	theme	soybean	336:342	arg1	lecithin					344:351	soybean lecithin	336:351	soybean lecithin	336:351	Solid-phase microextraction (SPME) and GC-MS were used to study the effects of varying concentrations of xanthan gum, sucrose fatty acid ester, Tween 80 and soybean lecithin on the release of seven aroma compounds.
27110762	4	30	theme	aroma	652:656	arg1	compounds					658:666	aroma compounds	652:666	aroma compounds	652:666	The results showed varying degrees of influence of sucrose fatty acid ester, soybean lecithin, Tween 80 and xanthan gum on the release of aroma compounds.
27110762	2	31	theme	xanthan	284:290	arg1	gum					292:294	xanthan gum	284:294	xanthan gum	284:294	Solid-phase microextraction (SPME) and GC-MS were used to study the effects of varying concentrations of xanthan gum, sucrose fatty acid ester, Tween 80 and soybean lecithin on the release of seven aroma compounds.
27110762	6	32	theme	aroma	989:993	arg1	molecules					995:1003	different aroma molecules	979:1003	different aroma molecules	979:1003	In conclusion, different emulsifiers and complexes showed different surface properties that tend to interact with different aroma molecules.
27110762	2	33	theme	aroma	377:381	arg1	compounds					383:391	seven aroma compounds	371:391	seven aroma compounds	371:391	Solid-phase microextraction (SPME) and GC-MS were used to study the effects of varying concentrations of xanthan gum, sucrose fatty acid ester, Tween 80 and soybean lecithin on the release of seven aroma compounds.
27110762	2	34	theme	compounds	383:391	arg1	release					360:366	the release	356:366	the release of seven aroma compounds	356:391	Solid-phase microextraction (SPME) and GC-MS were used to study the effects of varying concentrations of xanthan gum, sucrose fatty acid ester, Tween 80 and soybean lecithin on the release of seven aroma compounds.
27110762	4	35	theme	xanthan	622:628	arg1	gum					630:632	xanthan gum	622:632	xanthan gum	622:632	The results showed varying degrees of influence of sucrose fatty acid ester, soybean lecithin, Tween 80 and xanthan gum on the release of aroma compounds.
27110762	3	36	theme	systems	424:430	arg1	effects					398:404	The effects	394:404	The effects of the emulsifier systems supplemented with xanthan gum on aroma release	394:477	The effects of the emulsifier systems supplemented with xanthan gum on aroma release were also studied in the same way.
27110762	0	37	theme	Aroma	30:34	arg1	Release					36:42	Aroma Release	30:42	Aroma Release	30:42	Effect of Food Emulsifiers on Aroma Release.
27110762	2	38	theme	acid	311:314	arg1	ester					316:320	sucrose fatty acid ester	297:320	sucrose fatty acid ester	297:320	Solid-phase microextraction (SPME) and GC-MS were used to study the effects of varying concentrations of xanthan gum, sucrose fatty acid ester, Tween 80 and soybean lecithin on the release of seven aroma compounds.
27110762	5	39	theme	ethyl	706:710	arg1	acetate					712:718	ethyl acetate	706:718	ethyl acetate	706:718	Compared with other aroma compounds, ethyl acetate was more likely to be conserved in the solution system, while the amount of limonene released was the highest among these seven aroma compounds.
27110762	7	40	theme	emulsifiers	1071:1081	arg1	interactions					1096:1107	specific interactions	1087:1107	specific interactions between emulsifiers and aroma molecules	1087:1147	The present studies showed that the composition and structure of emulsifiers and specific interactions between emulsifiers and aroma molecules have significant effects on aroma release.
27110762	7	40	theme	emulsifiers	1071:1081	arg1	structure					1058:1066	structure	1058:1066	structure	1058:1066	The present studies showed that the composition and structure of emulsifiers and specific interactions between emulsifiers and aroma molecules have significant effects on aroma release.
27110762	7	40	theme	emulsifiers	1071:1081	arg1	composition					1042:1052	composition	1042:1052	composition	1042:1052	The present studies showed that the composition and structure of emulsifiers and specific interactions between emulsifiers and aroma molecules have significant effects on aroma release.
27110762	6	41	theme	different	923:931	arg1	properties					941:950	different surface properties	923:950	different surface properties that tend to interact with different aroma molecules	923:1003	In conclusion, different emulsifiers and complexes showed different surface properties that tend to interact with different aroma molecules.
27110762	5	42	theme	solution	759:766	arg1	system					768:773	the solution system	755:773	the solution system	755:773	Compared with other aroma compounds, ethyl acetate was more likely to be conserved in the solution system, while the amount of limonene released was the highest among these seven aroma compounds.
27110762	4	43	theme	ester	584:588	arg1	influence					552:560	influence	552:560	influence of sucrose fatty acid ester, soybean lecithin, Tween 80 and xanthan gum on the release of aroma compounds	552:666	The results showed varying degrees of influence of sucrose fatty acid ester, soybean lecithin, Tween 80 and xanthan gum on the release of aroma compounds.
27110762	4	44	theme	Tween	609:613	arg1	influence					552:560	influence	552:560	influence of sucrose fatty acid ester, soybean lecithin, Tween 80 and xanthan gum on the release of aroma compounds	552:666	The results showed varying degrees of influence of sucrose fatty acid ester, soybean lecithin, Tween 80 and xanthan gum on the release of aroma compounds.
27110762	5	45	theme	other	683:687	arg1	compounds					695:703	other aroma compounds	683:703	other aroma compounds	683:703	Compared with other aroma compounds, ethyl acetate was more likely to be conserved in the solution system, while the amount of limonene released was the highest among these seven aroma compounds.
27110762	7	46	dep	composition	1042:1052	arg1	the					1038:1040	the	1038:1040	the	1038:1040	The present studies showed that the composition and structure of emulsifiers and specific interactions between emulsifiers and aroma molecules have significant effects on aroma release.
27110762	2	47	theme	ester	316:320	arg1	concentrations					266:279	varying concentrations	258:279	varying concentrations of xanthan gum, sucrose fatty acid ester, Tween 80 and soybean lecithin	258:351	Solid-phase microextraction (SPME) and GC-MS were used to study the effects of varying concentrations of xanthan gum, sucrose fatty acid ester, Tween 80 and soybean lecithin on the release of seven aroma compounds.
27110762	5	48	theme	aroma	848:852	arg1	compounds					854:862	these seven aroma compounds	836:862	these seven aroma compounds	836:862	Compared with other aroma compounds, ethyl acetate was more likely to be conserved in the solution system, while the amount of limonene released was the highest among these seven aroma compounds.
27110762	1	49	theme	emulsifiers	102:112	arg1	influence					79:87	the influence	75:87	the influence of different emulsifiers or xanthan-emulsifier systems on the release of aroma compounds	75:176	This study aimed to determine the influence of different emulsifiers or xanthan-emulsifier systems on the release of aroma compounds.
27110762	1	50	from	influence	79:87	arg1	release					151:157	the release	147:157	the release of aroma compounds	147:176	This study aimed to determine the influence of different emulsifiers or xanthan-emulsifier systems on the release of aroma compounds.
27110762	7	51	contain	have	1149:1152	arg2	effects					1166:1172	significant effects	1154:1172	significant effects	1154:1172	The present studies showed that the composition and structure of emulsifiers and specific interactions between emulsifiers and aroma molecules have significant effects on aroma release.
27110762	7	51	contain	have	1149:1152	arg1	interactions					1096:1107	specific interactions	1087:1107	specific interactions between emulsifiers and aroma molecules	1087:1147	The present studies showed that the composition and structure of emulsifiers and specific interactions between emulsifiers and aroma molecules have significant effects on aroma release.
27110762	7	51	contain	have	1149:1152	arg1	structure					1058:1066	structure	1058:1066	structure	1058:1066	The present studies showed that the composition and structure of emulsifiers and specific interactions between emulsifiers and aroma molecules have significant effects on aroma release.
27110762	7	51	contain	have	1149:1152	arg1	composition					1042:1052	composition	1042:1052	composition	1042:1052	The present studies showed that the composition and structure of emulsifiers and specific interactions between emulsifiers and aroma molecules have significant effects on aroma release.
27110762	2	52	theme	fatty	305:309	arg1	ester					316:320	sucrose fatty acid ester	297:320	sucrose fatty acid ester	297:320	Solid-phase microextraction (SPME) and GC-MS were used to study the effects of varying concentrations of xanthan gum, sucrose fatty acid ester, Tween 80 and soybean lecithin on the release of seven aroma compounds.
27110762	2	53	from	effects	247:253	arg1	release					360:366	the release	356:366	the release of seven aroma compounds	356:391	Solid-phase microextraction (SPME) and GC-MS were used to study the effects of varying concentrations of xanthan gum, sucrose fatty acid ester, Tween 80 and soybean lecithin on the release of seven aroma compounds.
27110762	4	54	theme	influence	552:560	arg1	degrees					541:547	varying degrees	533:547	varying degrees of influence of sucrose fatty acid ester, soybean lecithin, Tween 80 and xanthan gum on the release of aroma compounds	533:666	The results showed varying degrees of influence of sucrose fatty acid ester, soybean lecithin, Tween 80 and xanthan gum on the release of aroma compounds.
27110762	1	55	theme	xanthan-emulsifier	117:134	arg1	systems					136:142	xanthan-emulsifier systems	117:142	xanthan-emulsifier systems	117:142	This study aimed to determine the influence of different emulsifiers or xanthan-emulsifier systems on the release of aroma compounds.
27110762	6	56	theme	surface	933:939	arg1	properties					941:950	different surface properties	923:950	different surface properties that tend to interact with different aroma molecules	923:1003	In conclusion, different emulsifiers and complexes showed different surface properties that tend to interact with different aroma molecules.
27110762	2	57	theme	Solid-phase	179:189	arg1	microextraction					191:205	Solid-phase microextraction	179:205	Solid-phase microextraction (SPME)	179:212	Solid-phase microextraction (SPME) and GC-MS were used to study the effects of varying concentrations of xanthan gum, sucrose fatty acid ester, Tween 80 and soybean lecithin on the release of seven aroma compounds.
27110762	2	57	theme	Solid-phase	179:189	arg1	SPME					208:211	SPME	208:211	SPME	208:211	Solid-phase microextraction (SPME) and GC-MS were used to study the effects of varying concentrations of xanthan gum, sucrose fatty acid ester, Tween 80 and soybean lecithin on the release of seven aroma compounds.
27110762	3	58	theme	emulsifier	413:422	arg1	systems					424:430	the emulsifier systems	409:430	the emulsifier systems supplemented with xanthan gum	409:460	The effects of the emulsifier systems supplemented with xanthan gum on aroma release were also studied in the same way.
27110762	1	59	theme	systems	136:142	arg1	influence					79:87	the influence	75:87	the influence of different emulsifiers or xanthan-emulsifier systems on the release of aroma compounds	75:176	This study aimed to determine the influence of different emulsifiers or xanthan-emulsifier systems on the release of aroma compounds.
27110762	4	60	theme	soybean	591:597	arg1	lecithin					599:606	soybean lecithin	591:606	soybean lecithin	591:606	The results showed varying degrees of influence of sucrose fatty acid ester, soybean lecithin, Tween 80 and xanthan gum on the release of aroma compounds.
27110762	4	61	theme	sucrose	565:571	arg1	ester					584:588	sucrose fatty acid ester	565:588	sucrose fatty acid ester	565:588	The results showed varying degrees of influence of sucrose fatty acid ester, soybean lecithin, Tween 80 and xanthan gum on the release of aroma compounds.
27110762	4	62	from	release	641:647	arg1	degrees					541:547	varying degrees	533:547	varying degrees of influence of sucrose fatty acid ester, soybean lecithin, Tween 80 and xanthan gum on the release of aroma compounds	533:666	The results showed varying degrees of influence of sucrose fatty acid ester, soybean lecithin, Tween 80 and xanthan gum on the release of aroma compounds.
27110762	0	63	theme	Food	10:13	arg1	Effect					0:5	Effect	0:5	Effect of Food	0:13	Effect of Food Emulsifiers on Aroma Release.
27110762	7	64	theme	specific	1087:1094	arg1	interactions					1096:1107	specific interactions	1087:1107	specific interactions between emulsifiers and aroma molecules	1087:1147	The present studies showed that the composition and structure of emulsifiers and specific interactions between emulsifiers and aroma molecules have significant effects on aroma release.
27110762	7	65	theme	aroma	1177:1181	arg1	release					1183:1189	aroma release	1177:1189	aroma release	1177:1189	The present studies showed that the composition and structure of emulsifiers and specific interactions between emulsifiers and aroma molecules have significant effects on aroma release.
24408274	5	0	theme	tuneable	846:853	arg1	properties					879:888	tuneable tensile and compressive properties	846:888	tuneable tensile and compressive properties	846:888	The method presented here provides a way to create large-scale biomimetic scaffolds that more closely mimic the composite structure of natural tissue, with tuneable tensile and compressive properties via the fibre and matrix phases, respectively.
24408274	1	1	theme	intervertebral	172:185	arg1	prone					203:207	prone	203:207	prone	203:207	Mechanically robust and biomimicking scaffolds are needed for structural engineering of tissues such as the intervertebral disc, which are prone to failure and incapable of natural healing.
24408274	1	1	theme	intervertebral	172:185	arg1	disc					187:190	the intervertebral disc	168:190	the intervertebral disc	168:190	Mechanically robust and biomimicking scaffolds are needed for structural engineering of tissues such as the intervertebral disc, which are prone to failure and incapable of natural healing.
24408274	4	2	theme	other	656:660	arg1	systems					681:687	other composite material systems	656:687	other composite material systems	656:687	The composites are mechanically robust and exhibit large strains-to-failure, exhibiting toughening mechanisms observed in other composite material systems.
24408274	5	3	theme	tensile	855:861	arg1	properties					879:888	tuneable tensile and compressive properties	846:888	tuneable tensile and compressive properties	846:888	The method presented here provides a way to create large-scale biomimetic scaffolds that more closely mimic the composite structure of natural tissue, with tuneable tensile and compressive properties via the fibre and matrix phases, respectively.
24408274	1	4	theme	robust	77:82	arg1	scaffolds					101:109	Mechanically robust and biomimicking scaffolds	64:109	Mechanically robust and biomimicking scaffolds	64:109	Mechanically robust and biomimicking scaffolds are needed for structural engineering of tissues such as the intervertebral disc, which are prone to failure and incapable of natural healing.
24408274	4	5	theme	material	672:679	arg1	systems					681:687	other composite material systems	656:687	other composite material systems	656:687	The composites are mechanically robust and exhibit large strains-to-failure, exhibiting toughening mechanisms observed in other composite material systems.
24408274	5	6	theme	large-scale	741:751	arg1	scaffolds					764:772	large-scale biomimetic scaffolds	741:772	large-scale biomimetic scaffolds that more closely mimic the composite structure of natural tissue, with tuneable tensile and compressive properties via the fibre and matrix phases, respectively	741:934	The method presented here provides a way to create large-scale biomimetic scaffolds that more closely mimic the composite structure of natural tissue, with tuneable tensile and compressive properties via the fibre and matrix phases, respectively.
24408274	4	7	theme	composite	662:670	arg1	systems					681:687	other composite material systems	656:687	other composite material systems	656:687	The composites are mechanically robust and exhibit large strains-to-failure, exhibiting toughening mechanisms observed in other composite material systems.
24408274	5	8	theme	natural	825:831	arg1	tissue					833:838	natural tissue	825:838	natural tissue	825:838	The method presented here provides a way to create large-scale biomimetic scaffolds that more closely mimic the composite structure of natural tissue, with tuneable tensile and compressive properties via the fibre and matrix phases, respectively.
24408274	1	9	theme	biomimicking	88:99	arg1	scaffolds					101:109	Mechanically robust and biomimicking scaffolds	64:109	Mechanically robust and biomimicking scaffolds	64:109	Mechanically robust and biomimicking scaffolds are needed for structural engineering of tissues such as the intervertebral disc, which are prone to failure and incapable of natural healing.
24408274	5	10	theme	compressive	867:877	arg1	properties					879:888	tuneable tensile and compressive properties	846:888	tuneable tensile and compressive properties	846:888	The method presented here provides a way to create large-scale biomimetic scaffolds that more closely mimic the composite structure of natural tissue, with tuneable tensile and compressive properties via the fibre and matrix phases, respectively.
24408274	5	11	theme	tissue	833:838	arg1	structure					812:820	the composite structure	798:820	the composite structure of natural tissue	798:838	The method presented here provides a way to create large-scale biomimetic scaffolds that more closely mimic the composite structure of natural tissue, with tuneable tensile and compressive properties via the fibre and matrix phases, respectively.
24408274	4	12	located	observed	644:651	arg2	mechanisms					633:642	toughening mechanisms	622:642	toughening mechanisms observed in other composite material systems	622:687	The composites are mechanically robust and exhibit large strains-to-failure, exhibiting toughening mechanisms observed in other composite material systems.
24408274	4	12	located	observed	644:651	arg1	systems					681:687	other composite material systems	656:687	other composite material systems	656:687	The composites are mechanically robust and exhibit large strains-to-failure, exhibiting toughening mechanisms observed in other composite material systems.
24408274	2	13	theme	fibre	330:334	arg1	structures					336:345	thick, randomly aligned polycaprolactone electrospun fibre structures	277:345	thick, randomly aligned polycaprolactone electrospun fibre structures infiltrated with alginate	277:371	Here, the formation of thick, randomly aligned polycaprolactone electrospun fibre structures infiltrated with alginate is reported.
24408274	0	14	theme	Mechanical	0:9	arg1	behaviour					11:19	Mechanical behaviour	0:19	Mechanical behaviour of electrospun	0:34	Mechanical behaviour of electrospun fibre-reinforced hydrogels.
24408274	4	15	theme	toughening	622:631	arg1	mechanisms					633:642	toughening mechanisms	622:642	toughening mechanisms observed in other composite material systems	622:687	The composites are mechanically robust and exhibit large strains-to-failure, exhibiting toughening mechanisms observed in other composite material systems.
24408274	2	16	theme	electrospun	318:328	arg1	structures					336:345	thick, randomly aligned polycaprolactone electrospun fibre structures	277:345	thick, randomly aligned polycaprolactone electrospun fibre structures infiltrated with alginate	277:371	Here, the formation of thick, randomly aligned polycaprolactone electrospun fibre structures infiltrated with alginate is reported.
24408274	0	17	theme	electrospun	24:34	arg1	behaviour					11:19	Mechanical behaviour	0:19	Mechanical behaviour of electrospun	0:34	Mechanical behaviour of electrospun fibre-reinforced hydrogels.
24408274	5	18	theme	fibre	898:902	arg1	phases					915:920	the fibre and matrix phases	894:920	phases	915:920	The method presented here provides a way to create large-scale biomimetic scaffolds that more closely mimic the composite structure of natural tissue, with tuneable tensile and compressive properties via the fibre and matrix phases, respectively.
24408274	2	19	theme	polycaprolactone	301:316	arg1	structures					336:345	thick, randomly aligned polycaprolactone electrospun fibre structures	277:345	thick, randomly aligned polycaprolactone electrospun fibre structures infiltrated with alginate	277:371	Here, the formation of thick, randomly aligned polycaprolactone electrospun fibre structures infiltrated with alginate is reported.
24408274	5	20	theme	matrix	908:913	arg1	phases					915:920	the fibre and matrix phases	894:920	phases	915:920	The method presented here provides a way to create large-scale biomimetic scaffolds that more closely mimic the composite structure of natural tissue, with tuneable tensile and compressive properties via the fibre and matrix phases, respectively.
24408274	2	21	theme	thick	277:281	arg1	structures					336:345	thick, randomly aligned polycaprolactone electrospun fibre structures	277:345	thick, randomly aligned polycaprolactone electrospun fibre structures infiltrated with alginate	277:371	Here, the formation of thick, randomly aligned polycaprolactone electrospun fibre structures infiltrated with alginate is reported.
24408274	1	22	theme	structural	126:135	arg1	engineering					137:147	structural engineering	126:147	structural engineering of tissues such as the intervertebral disc, which are prone to failure and incapable of natural healing	126:251	Mechanically robust and biomimicking scaffolds are needed for structural engineering of tissues such as the intervertebral disc, which are prone to failure and incapable of natural healing.
24408274	3	23	theme	compressive	514:524	arg1	moduli					526:531	substantially different tensile and compressive moduli	478:531	substantially different tensile and compressive moduli	478:531	The composites are characterised using both indentation and tensile testing and demonstrate substantially different tensile and compressive moduli.
24408274	3	24	theme	different	492:500	arg1	moduli					526:531	substantially different tensile and compressive moduli	478:531	substantially different tensile and compressive moduli	478:531	The composites are characterised using both indentation and tensile testing and demonstrate substantially different tensile and compressive moduli.
24408274	3	25	theme	tensile	446:452	arg1	testing					454:460	tensile testing	446:460	tensile testing	446:460	The composites are characterised using both indentation and tensile testing and demonstrate substantially different tensile and compressive moduli.
24408274	4	26	theme	large	585:589	arg1	strains-to-failure					591:608	large strains-to-failure	585:608	large strains-to-failure	585:608	The composites are mechanically robust and exhibit large strains-to-failure, exhibiting toughening mechanisms observed in other composite material systems.
24408274	5	27	theme	composite	802:810	arg1	structure					812:820	the composite structure	798:820	the composite structure of natural tissue	798:838	The method presented here provides a way to create large-scale biomimetic scaffolds that more closely mimic the composite structure of natural tissue, with tuneable tensile and compressive properties via the fibre and matrix phases, respectively.
24408274	3	28	theme	tensile	502:508	arg1	moduli					526:531	substantially different tensile and compressive moduli	478:531	substantially different tensile and compressive moduli	478:531	The composites are characterised using both indentation and tensile testing and demonstrate substantially different tensile and compressive moduli.
24408274	5	29	theme	biomimetic	753:762	arg1	scaffolds					764:772	large-scale biomimetic scaffolds	741:772	large-scale biomimetic scaffolds that more closely mimic the composite structure of natural tissue, with tuneable tensile and compressive properties via the fibre and matrix phases, respectively	741:934	The method presented here provides a way to create large-scale biomimetic scaffolds that more closely mimic the composite structure of natural tissue, with tuneable tensile and compressive properties via the fibre and matrix phases, respectively.
24408274	1	30	theme	tissues	152:158	arg1	engineering					137:147	structural engineering	126:147	structural engineering of tissues such as the intervertebral disc, which are prone to failure and incapable of natural healing	126:251	Mechanically robust and biomimicking scaffolds are needed for structural engineering of tissues such as the intervertebral disc, which are prone to failure and incapable of natural healing.
24408274	2	31	theme	structures	336:345	arg1	formation					264:272	the formation	260:272	the formation of thick, randomly aligned polycaprolactone electrospun fibre structures infiltrated with alginate	260:371	Here, the formation of thick, randomly aligned polycaprolactone electrospun fibre structures infiltrated with alginate is reported.
24408274	1	32	theme	natural	237:243	arg1	healing					245:251	natural healing	237:251	natural healing	237:251	Mechanically robust and biomimicking scaffolds are needed for structural engineering of tissues such as the intervertebral disc, which are prone to failure and incapable of natural healing.
24408274	2	33	dep	thick	277:281	arg1	aligned					293:299	aligned	293:299	aligned	293:299	Here, the formation of thick, randomly aligned polycaprolactone electrospun fibre structures infiltrated with alginate is reported.
24408274	1	34	theme	healing	245:251	arg1	incapable					224:232	incapable	224:232	incapable	224:232	Mechanically robust and biomimicking scaffolds are needed for structural engineering of tissues such as the intervertebral disc, which are prone to failure and incapable of natural healing.
28013138	1	0	theme	extracellular	320:332	arg1	polysaccharide					334:347	a value-added fucose-rich extracellular polysaccharide	294:347	a value-added fucose-rich extracellular polysaccharide	294:347	Out-of-specification tomato paste, a by-product from the tomato processing industry, was used as the sole substrate for cultivation of the bacterium Enterobacter A47 and production of FucoPol, a value-added fucose-rich extracellular polysaccharide.
28013138	1	0	theme	extracellular	320:332	arg1	substrate					207:215	the sole substrate	198:215	the sole substrate for cultivation of the bacterium Enterobacter A47 and production of FucoPol	198:291	Out-of-specification tomato paste, a by-product from the tomato processing industry, was used as the sole substrate for cultivation of the bacterium Enterobacter A47 and production of FucoPol, a value-added fucose-rich extracellular polysaccharide.
28013138	4	1	theme	polydispersity	841:854	arg1	1.2					866:868	1.2	866:868	1.2	866:868	The average molecular weight was 4.4×106Da and the polydispersity index was 1.2.
28013138	4	1	theme	polydispersity	841:854	arg1	index					856:860	the polydispersity index	837:860	the polydispersity index	837:860	The average molecular weight was 4.4×106Da and the polydispersity index was 1.2.
28013138	3	2	theme	acyl	759:762	arg1	groups					764:769	acyl groups	759:769	an acyl groups content of 13wt%	756:786	The polymer produced had the typical FucoPol composition (37mol% fucose, 27mol% galactose, 23mol% glucose and 12mol% glucuronic acid, with an acyl groups content of 13wt%).
28013138	3	3	theme	27mol	690:694	arg1	galactose					697:705	27mol% galactose	690:705	27mol% galactose	690:705	The polymer produced had the typical FucoPol composition (37mol% fucose, 27mol% galactose, 23mol% glucose and 12mol% glucuronic acid, with an acyl groups content of 13wt%).
28013138	3	4	theme	%	680:680	arg1	fucose					682:687	37mol% fucose	675:687	37mol% fucose	675:687	The polymer produced had the typical FucoPol composition (37mol% fucose, 27mol% galactose, 23mol% glucose and 12mol% glucuronic acid, with an acyl groups content of 13wt%).
28013138	4	5	theme	molecular	802:810	arg1	weight					812:817	The average molecular weight	790:817	The average molecular weight	790:817	The average molecular weight was 4.4×106Da and the polydispersity index was 1.2.
28013138	4	5	theme	molecular	802:810	arg1	4.4×106Da					823:831	4.4×106Da	823:831	4.4×106Da	823:831	The average molecular weight was 4.4×106Da and the polydispersity index was 1.2.
28013138	3	6	with	%	732:732	arg1	content					771:777	an acyl groups content	756:777	an acyl groups content of 13wt%	756:786	The polymer produced had the typical FucoPol composition (37mol% fucose, 27mol% galactose, 23mol% glucose and 12mol% glucuronic acid, with an acyl groups content of 13wt%).
28013138	3	7	theme	%	786:786	arg1	content					771:777	an acyl groups content	756:777	an acyl groups content of 13wt%	756:786	The polymer produced had the typical FucoPol composition (37mol% fucose, 27mol% galactose, 23mol% glucose and 12mol% glucuronic acid, with an acyl groups content of 13wt%).
28013138	0	8	theme	carbon	86:91	arg1	source					93:98	sole carbon source	81:98	sole carbon source	81:98	Production of FucoPol by Enterobacter A47 using waste tomato paste by-product as sole carbon source.
28013138	3	9	with	glucose	715:721	arg1	content					771:777	an acyl groups content	756:777	an acyl groups content of 13wt%	756:786	The polymer produced had the typical FucoPol composition (37mol% fucose, 27mol% galactose, 23mol% glucose and 12mol% glucuronic acid, with an acyl groups content of 13wt%).
28013138	2	10	theme	flow	596:599	arg1	rate					601:604	a constant flow rate	585:604	a constant flow rate of 11gh-1	585:614	Among the different tested fed-batch strategies, pH-stat, DO-stat and continuous substrate feeding, the highest production (8.77gL-1) and overall volumetric productivity (2.92gL-1d-1) were obtained with continuous substrate feeding at a constant flow rate of 11gh-1.
28013138	5	11	theme	tomato	921:926	arg1	substrate					957:965	a suitable low-cost substrate	937:965	a suitable low-cost substrate for the production of FucoPol	937:995	This study demonstrated that out-of-specification tomato paste is a suitable low-cost substrate for the production of FucoPol, thus providing a route for the valorization of this by-product into a high-value microbial product.
28013138	5	11	theme	tomato	921:926	arg1	paste					928:932	out-of-specification tomato paste	900:932	out-of-specification tomato paste	900:932	This study demonstrated that out-of-specification tomato paste is a suitable low-cost substrate for the production of FucoPol, thus providing a route for the valorization of this by-product into a high-value microbial product.
28013138	1	12	theme	bacterium	240:248	arg1	A47					263:265	the bacterium Enterobacter A47	236:265	the bacterium Enterobacter A47	236:265	Out-of-specification tomato paste, a by-product from the tomato processing industry, was used as the sole substrate for cultivation of the bacterium Enterobacter A47 and production of FucoPol, a value-added fucose-rich extracellular polysaccharide.
28013138	0	13	theme	sole	81:84	arg1	source					93:98	sole carbon source	81:98	sole carbon source	81:98	Production of FucoPol by Enterobacter A47 using waste tomato paste by-product as sole carbon source.
28013138	2	14	theme	constant	587:594	arg1	rate					601:604	a constant flow rate	585:604	a constant flow rate of 11gh-1	585:614	Among the different tested fed-batch strategies, pH-stat, DO-stat and continuous substrate feeding, the highest production (8.77gL-1) and overall volumetric productivity (2.92gL-1d-1) were obtained with continuous substrate feeding at a constant flow rate of 11gh-1.
28013138	1	15	theme	Enterobacter	250:261	arg1	A47					263:265	the bacterium Enterobacter A47	236:265	the bacterium Enterobacter A47	236:265	Out-of-specification tomato paste, a by-product from the tomato processing industry, was used as the sole substrate for cultivation of the bacterium Enterobacter A47 and production of FucoPol, a value-added fucose-rich extracellular polysaccharide.
28013138	3	16	theme	23mol	708:712	arg1	glucose					715:721	23mol% glucose	708:721	23mol% glucose	708:721	The polymer produced had the typical FucoPol composition (37mol% fucose, 27mol% galactose, 23mol% glucose and 12mol% glucuronic acid, with an acyl groups content of 13wt%).
28013138	3	17	with	fucose	682:687	arg1	content					771:777	an acyl groups content	756:777	an acyl groups content of 13wt%	756:786	The polymer produced had the typical FucoPol composition (37mol% fucose, 27mol% galactose, 23mol% glucose and 12mol% glucuronic acid, with an acyl groups content of 13wt%).
28013138	1	18	theme	tomato	158:163	arg1	industry					176:183	the tomato processing industry	154:183	the tomato processing industry	154:183	Out-of-specification tomato paste, a by-product from the tomato processing industry, was used as the sole substrate for cultivation of the bacterium Enterobacter A47 and production of FucoPol, a value-added fucose-rich extracellular polysaccharide.
28013138	3	19	theme	%	695:695	arg1	galactose					697:705	27mol% galactose	690:705	27mol% galactose	690:705	The polymer produced had the typical FucoPol composition (37mol% fucose, 27mol% galactose, 23mol% glucose and 12mol% glucuronic acid, with an acyl groups content of 13wt%).
28013138	5	20	theme	high-value	1068:1077	arg1	product					1089:1095	a high-value microbial product	1066:1095	a high-value microbial product	1066:1095	This study demonstrated that out-of-specification tomato paste is a suitable low-cost substrate for the production of FucoPol, thus providing a route for the valorization of this by-product into a high-value microbial product.
28013138	1	21	theme	processing	165:174	arg1	industry					176:183	the tomato processing industry	154:183	the tomato processing industry	154:183	Out-of-specification tomato paste, a by-product from the tomato processing industry, was used as the sole substrate for cultivation of the bacterium Enterobacter A47 and production of FucoPol, a value-added fucose-rich extracellular polysaccharide.
28013138	4	22	theme	average	794:800	arg1	weight					812:817	The average molecular weight	790:817	The average molecular weight	790:817	The average molecular weight was 4.4×106Da and the polydispersity index was 1.2.
28013138	4	22	theme	average	794:800	arg1	4.4×106Da					823:831	4.4×106Da	823:831	4.4×106Da	823:831	The average molecular weight was 4.4×106Da and the polydispersity index was 1.2.
28013138	2	23	theme	volumetric	496:505	arg1	2.92gL-1d-1					521:531	2.92gL-1d-1	521:531	2.92gL-1d-1	521:531	Among the different tested fed-batch strategies, pH-stat, DO-stat and continuous substrate feeding, the highest production (8.77gL-1) and overall volumetric productivity (2.92gL-1d-1) were obtained with continuous substrate feeding at a constant flow rate of 11gh-1.
28013138	2	23	theme	volumetric	496:505	arg1	productivity					507:518	overall volumetric productivity	488:518	overall volumetric productivity (2.92gL-1d-1)	488:532	Among the different tested fed-batch strategies, pH-stat, DO-stat and continuous substrate feeding, the highest production (8.77gL-1) and overall volumetric productivity (2.92gL-1d-1) were obtained with continuous substrate feeding at a constant flow rate of 11gh-1.
28013138	3	24	dep	fucose	682:687	arg1	acid					745:748	glucuronic acid	734:748	glucuronic acid	734:748	The polymer produced had the typical FucoPol composition (37mol% fucose, 27mol% galactose, 23mol% glucose and 12mol% glucuronic acid, with an acyl groups content of 13wt%).
28013138	1	25	theme	A47	263:265	arg1	production					271:280	production	271:280	production of FucoPol	271:291	Out-of-specification tomato paste, a by-product from the tomato processing industry, was used as the sole substrate for cultivation of the bacterium Enterobacter A47 and production of FucoPol, a value-added fucose-rich extracellular polysaccharide.
28013138	1	25	theme	A47	263:265	arg1	cultivation					221:231	cultivation	221:231	cultivation of the bacterium Enterobacter A47	221:265	Out-of-specification tomato paste, a by-product from the tomato processing industry, was used as the sole substrate for cultivation of the bacterium Enterobacter A47 and production of FucoPol, a value-added fucose-rich extracellular polysaccharide.
28013138	2	26	theme	11gh-1	609:614	arg1	rate					601:604	a constant flow rate	585:604	a constant flow rate of 11gh-1	585:614	Among the different tested fed-batch strategies, pH-stat, DO-stat and continuous substrate feeding, the highest production (8.77gL-1) and overall volumetric productivity (2.92gL-1d-1) were obtained with continuous substrate feeding at a constant flow rate of 11gh-1.
28013138	5	27	theme	microbial	1079:1087	arg1	product					1089:1095	a high-value microbial product	1066:1095	a high-value microbial product	1066:1095	This study demonstrated that out-of-specification tomato paste is a suitable low-cost substrate for the production of FucoPol, thus providing a route for the valorization of this by-product into a high-value microbial product.
28013138	2	28	theme	fed-batch	377:385	arg1	strategies					387:396	the different tested fed-batch strategies	356:396	the different tested fed-batch strategies	356:396	Among the different tested fed-batch strategies, pH-stat, DO-stat and continuous substrate feeding, the highest production (8.77gL-1) and overall volumetric productivity (2.92gL-1d-1) were obtained with continuous substrate feeding at a constant flow rate of 11gh-1.
28013138	3	29	theme	12mol	727:731	arg1	%					732:732	12mol%	727:732	12mol%	727:732	The polymer produced had the typical FucoPol composition (37mol% fucose, 27mol% galactose, 23mol% glucose and 12mol% glucuronic acid, with an acyl groups content of 13wt%).
28013138	2	30	theme	continuous	553:562	arg1	feeding					574:580	continuous substrate feeding	553:580	continuous substrate feeding	553:580	Among the different tested fed-batch strategies, pH-stat, DO-stat and continuous substrate feeding, the highest production (8.77gL-1) and overall volumetric productivity (2.92gL-1d-1) were obtained with continuous substrate feeding at a constant flow rate of 11gh-1.
28013138	2	31	theme	continuous	420:429	arg1	feeding					441:447	continuous substrate feeding	420:447	continuous substrate feeding	420:447	Among the different tested fed-batch strategies, pH-stat, DO-stat and continuous substrate feeding, the highest production (8.77gL-1) and overall volumetric productivity (2.92gL-1d-1) were obtained with continuous substrate feeding at a constant flow rate of 11gh-1.
28013138	0	32	theme	FucoPol	14:20	arg1	Production					0:9	Production	0:9	Production of FucoPol by Enterobacter A47 using waste tomato paste by-product as sole carbon source.	0:99	Production of FucoPol by Enterobacter A47 using waste tomato paste by-product as sole carbon source.
28013138	3	33	theme	%	713:713	arg1	glucose					715:721	23mol% glucose	708:721	23mol% glucose	708:721	The polymer produced had the typical FucoPol composition (37mol% fucose, 27mol% galactose, 23mol% glucose and 12mol% glucuronic acid, with an acyl groups content of 13wt%).
28013138	5	34	theme	out-of-specification	900:919	arg1	substrate					957:965	a suitable low-cost substrate	937:965	a suitable low-cost substrate for the production of FucoPol	937:995	This study demonstrated that out-of-specification tomato paste is a suitable low-cost substrate for the production of FucoPol, thus providing a route for the valorization of this by-product into a high-value microbial product.
28013138	5	34	theme	out-of-specification	900:919	arg1	paste					928:932	out-of-specification tomato paste	900:932	out-of-specification tomato paste	900:932	This study demonstrated that out-of-specification tomato paste is a suitable low-cost substrate for the production of FucoPol, thus providing a route for the valorization of this by-product into a high-value microbial product.
28013138	3	35	theme	typical	646:652	arg1	composition					662:672	the typical FucoPol composition	642:672	the typical FucoPol composition (37mol% fucose, 27mol% galactose, 23mol% glucose and 12mol% glucuronic acid, with an acyl groups content of 13wt%)	642:787	The polymer produced had the typical FucoPol composition (37mol% fucose, 27mol% galactose, 23mol% glucose and 12mol% glucuronic acid, with an acyl groups content of 13wt%).
28013138	5	36	dep	suitable	939:946	arg1	low-cost					948:955	low-cost	948:955	low-cost	948:955	This study demonstrated that out-of-specification tomato paste is a suitable low-cost substrate for the production of FucoPol, thus providing a route for the valorization of this by-product into a high-value microbial product.
28013138	1	37	theme	FucoPol	285:291	arg1	production					271:280	production	271:280	production of FucoPol	271:291	Out-of-specification tomato paste, a by-product from the tomato processing industry, was used as the sole substrate for cultivation of the bacterium Enterobacter A47 and production of FucoPol, a value-added fucose-rich extracellular polysaccharide.
28013138	1	37	theme	FucoPol	285:291	arg1	cultivation					221:231	cultivation	221:231	cultivation of the bacterium Enterobacter A47	221:265	Out-of-specification tomato paste, a by-product from the tomato processing industry, was used as the sole substrate for cultivation of the bacterium Enterobacter A47 and production of FucoPol, a value-added fucose-rich extracellular polysaccharide.
28013138	3	38	dep	composition	662:672	arg1	galactose					697:705	27mol% galactose	690:705	27mol% galactose	690:705	The polymer produced had the typical FucoPol composition (37mol% fucose, 27mol% galactose, 23mol% glucose and 12mol% glucuronic acid, with an acyl groups content of 13wt%).
28013138	3	38	dep	composition	662:672	arg1	glucose					715:721	23mol% glucose	708:721	23mol% glucose	708:721	The polymer produced had the typical FucoPol composition (37mol% fucose, 27mol% galactose, 23mol% glucose and 12mol% glucuronic acid, with an acyl groups content of 13wt%).
28013138	3	38	dep	composition	662:672	arg1	%					732:732	12mol%	727:732	12mol%	727:732	The polymer produced had the typical FucoPol composition (37mol% fucose, 27mol% galactose, 23mol% glucose and 12mol% glucuronic acid, with an acyl groups content of 13wt%).
28013138	3	38	dep	composition	662:672	arg1	fucose					682:687	37mol% fucose	675:687	37mol% fucose	675:687	The polymer produced had the typical FucoPol composition (37mol% fucose, 27mol% galactose, 23mol% glucose and 12mol% glucuronic acid, with an acyl groups content of 13wt%).
28013138	3	39	theme	glucuronic	734:743	arg1	acid					745:748	glucuronic acid	734:748	glucuronic acid	734:748	The polymer produced had the typical FucoPol composition (37mol% fucose, 27mol% galactose, 23mol% glucose and 12mol% glucuronic acid, with an acyl groups content of 13wt%).
28013138	0	40	theme	waste	48:52	arg1	by-product					67:76	waste tomato paste by-product	48:76	waste tomato paste by-product	48:76	Production of FucoPol by Enterobacter A47 using waste tomato paste by-product as sole carbon source.
28013138	2	41	theme	overall	488:494	arg1	2.92gL-1d-1					521:531	2.92gL-1d-1	521:531	2.92gL-1d-1	521:531	Among the different tested fed-batch strategies, pH-stat, DO-stat and continuous substrate feeding, the highest production (8.77gL-1) and overall volumetric productivity (2.92gL-1d-1) were obtained with continuous substrate feeding at a constant flow rate of 11gh-1.
28013138	2	41	theme	overall	488:494	arg1	productivity					507:518	overall volumetric productivity	488:518	overall volumetric productivity (2.92gL-1d-1)	488:532	Among the different tested fed-batch strategies, pH-stat, DO-stat and continuous substrate feeding, the highest production (8.77gL-1) and overall volumetric productivity (2.92gL-1d-1) were obtained with continuous substrate feeding at a constant flow rate of 11gh-1.
28013138	1	42	used	used	190:193	arg2	by-product					138:147	a by-product	136:147	a by-product from the tomato processing industry	136:183	Out-of-specification tomato paste, a by-product from the tomato processing industry, was used as the sole substrate for cultivation of the bacterium Enterobacter A47 and production of FucoPol, a value-added fucose-rich extracellular polysaccharide.
28013138	1	42	used	used	190:193	arg2	substrate					207:215	the sole substrate	198:215	the sole substrate for cultivation of the bacterium Enterobacter A47 and production of FucoPol	198:291	Out-of-specification tomato paste, a by-product from the tomato processing industry, was used as the sole substrate for cultivation of the bacterium Enterobacter A47 and production of FucoPol, a value-added fucose-rich extracellular polysaccharide.
28013138	1	42	used	used	190:193	arg2	paste					129:133	Out-of-specification tomato paste	101:133	Out-of-specification tomato paste	101:133	Out-of-specification tomato paste, a by-product from the tomato processing industry, was used as the sole substrate for cultivation of the bacterium Enterobacter A47 and production of FucoPol, a value-added fucose-rich extracellular polysaccharide.
28013138	5	43	theme	by-product	1050:1059	arg1	valorization					1029:1040	the valorization	1025:1040	the valorization of this by-product	1025:1059	This study demonstrated that out-of-specification tomato paste is a suitable low-cost substrate for the production of FucoPol, thus providing a route for the valorization of this by-product into a high-value microbial product.
28013138	1	44	theme	Out-of-specification	101:120	arg1	paste					129:133	Out-of-specification tomato paste	101:133	Out-of-specification tomato paste	101:133	Out-of-specification tomato paste, a by-product from the tomato processing industry, was used as the sole substrate for cultivation of the bacterium Enterobacter A47 and production of FucoPol, a value-added fucose-rich extracellular polysaccharide.
28013138	1	44	theme	Out-of-specification	101:120	arg1	by-product					138:147	a by-product	136:147	a by-product from the tomato processing industry	136:183	Out-of-specification tomato paste, a by-product from the tomato processing industry, was used as the sole substrate for cultivation of the bacterium Enterobacter A47 and production of FucoPol, a value-added fucose-rich extracellular polysaccharide.
28013138	1	44	theme	Out-of-specification	101:120	arg1	substrate					207:215	the sole substrate	198:215	the sole substrate for cultivation of the bacterium Enterobacter A47 and production of FucoPol	198:291	Out-of-specification tomato paste, a by-product from the tomato processing industry, was used as the sole substrate for cultivation of the bacterium Enterobacter A47 and production of FucoPol, a value-added fucose-rich extracellular polysaccharide.
28013138	2	45	theme	highest	454:460	arg1	production					462:471	the highest production	450:471	the highest production (8.77gL-1)	450:482	Among the different tested fed-batch strategies, pH-stat, DO-stat and continuous substrate feeding, the highest production (8.77gL-1) and overall volumetric productivity (2.92gL-1d-1) were obtained with continuous substrate feeding at a constant flow rate of 11gh-1.
28013138	2	45	theme	highest	454:460	arg1	8.77gL-1					474:481	8.77gL-1	474:481	8.77gL-1	474:481	Among the different tested fed-batch strategies, pH-stat, DO-stat and continuous substrate feeding, the highest production (8.77gL-1) and overall volumetric productivity (2.92gL-1d-1) were obtained with continuous substrate feeding at a constant flow rate of 11gh-1.
28013138	5	46	theme	FucoPol	989:995	arg1	production					975:984	the production	971:984	the production of FucoPol	971:995	This study demonstrated that out-of-specification tomato paste is a suitable low-cost substrate for the production of FucoPol, thus providing a route for the valorization of this by-product into a high-value microbial product.
28013138	1	47	theme	tomato	122:127	arg1	paste					129:133	Out-of-specification tomato paste	101:133	Out-of-specification tomato paste	101:133	Out-of-specification tomato paste, a by-product from the tomato processing industry, was used as the sole substrate for cultivation of the bacterium Enterobacter A47 and production of FucoPol, a value-added fucose-rich extracellular polysaccharide.
28013138	1	47	theme	tomato	122:127	arg1	by-product					138:147	a by-product	136:147	a by-product from the tomato processing industry	136:183	Out-of-specification tomato paste, a by-product from the tomato processing industry, was used as the sole substrate for cultivation of the bacterium Enterobacter A47 and production of FucoPol, a value-added fucose-rich extracellular polysaccharide.
28013138	1	47	theme	tomato	122:127	arg1	substrate					207:215	the sole substrate	198:215	the sole substrate for cultivation of the bacterium Enterobacter A47 and production of FucoPol	198:291	Out-of-specification tomato paste, a by-product from the tomato processing industry, was used as the sole substrate for cultivation of the bacterium Enterobacter A47 and production of FucoPol, a value-added fucose-rich extracellular polysaccharide.
28013138	1	48	theme	value-added	296:306	arg1	polysaccharide					334:347	a value-added fucose-rich extracellular polysaccharide	294:347	a value-added fucose-rich extracellular polysaccharide	294:347	Out-of-specification tomato paste, a by-product from the tomato processing industry, was used as the sole substrate for cultivation of the bacterium Enterobacter A47 and production of FucoPol, a value-added fucose-rich extracellular polysaccharide.
28013138	1	48	theme	value-added	296:306	arg1	substrate					207:215	the sole substrate	198:215	the sole substrate for cultivation of the bacterium Enterobacter A47 and production of FucoPol	198:291	Out-of-specification tomato paste, a by-product from the tomato processing industry, was used as the sole substrate for cultivation of the bacterium Enterobacter A47 and production of FucoPol, a value-added fucose-rich extracellular polysaccharide.
28013138	3	49	theme	37mol	675:679	arg1	fucose					682:687	37mol% fucose	675:687	37mol% fucose	675:687	The polymer produced had the typical FucoPol composition (37mol% fucose, 27mol% galactose, 23mol% glucose and 12mol% glucuronic acid, with an acyl groups content of 13wt%).
28013138	5	50	theme	suitable	939:946	arg1	substrate					957:965	a suitable low-cost substrate	937:965	a suitable low-cost substrate for the production of FucoPol	937:995	This study demonstrated that out-of-specification tomato paste is a suitable low-cost substrate for the production of FucoPol, thus providing a route for the valorization of this by-product into a high-value microbial product.
28013138	5	50	theme	suitable	939:946	arg1	paste					928:932	out-of-specification tomato paste	900:932	out-of-specification tomato paste	900:932	This study demonstrated that out-of-specification tomato paste is a suitable low-cost substrate for the production of FucoPol, thus providing a route for the valorization of this by-product into a high-value microbial product.
28013138	3	51	with	galactose	697:705	arg1	content					771:777	an acyl groups content	756:777	an acyl groups content of 13wt%	756:786	The polymer produced had the typical FucoPol composition (37mol% fucose, 27mol% galactose, 23mol% glucose and 12mol% glucuronic acid, with an acyl groups content of 13wt%).
28013138	1	52	theme	sole	202:205	arg1	polysaccharide					334:347	a value-added fucose-rich extracellular polysaccharide	294:347	a value-added fucose-rich extracellular polysaccharide	294:347	Out-of-specification tomato paste, a by-product from the tomato processing industry, was used as the sole substrate for cultivation of the bacterium Enterobacter A47 and production of FucoPol, a value-added fucose-rich extracellular polysaccharide.
28013138	1	52	theme	sole	202:205	arg1	paste					129:133	Out-of-specification tomato paste	101:133	Out-of-specification tomato paste	101:133	Out-of-specification tomato paste, a by-product from the tomato processing industry, was used as the sole substrate for cultivation of the bacterium Enterobacter A47 and production of FucoPol, a value-added fucose-rich extracellular polysaccharide.
28013138	1	52	theme	sole	202:205	arg1	substrate					207:215	the sole substrate	198:215	the sole substrate for cultivation of the bacterium Enterobacter A47 and production of FucoPol	198:291	Out-of-specification tomato paste, a by-product from the tomato processing industry, was used as the sole substrate for cultivation of the bacterium Enterobacter A47 and production of FucoPol, a value-added fucose-rich extracellular polysaccharide.
28013138	2	53	theme	substrate	564:572	arg1	feeding					574:580	continuous substrate feeding	553:580	continuous substrate feeding	553:580	Among the different tested fed-batch strategies, pH-stat, DO-stat and continuous substrate feeding, the highest production (8.77gL-1) and overall volumetric productivity (2.92gL-1d-1) were obtained with continuous substrate feeding at a constant flow rate of 11gh-1.
28013138	3	54	theme	FucoPol	654:660	arg1	composition					662:672	the typical FucoPol composition	642:672	the typical FucoPol composition (37mol% fucose, 27mol% galactose, 23mol% glucose and 12mol% glucuronic acid, with an acyl groups content of 13wt%)	642:787	The polymer produced had the typical FucoPol composition (37mol% fucose, 27mol% galactose, 23mol% glucose and 12mol% glucuronic acid, with an acyl groups content of 13wt%).
28013138	1	55	from	industry	176:183	arg1	paste					129:133	Out-of-specification tomato paste	101:133	Out-of-specification tomato paste	101:133	Out-of-specification tomato paste, a by-product from the tomato processing industry, was used as the sole substrate for cultivation of the bacterium Enterobacter A47 and production of FucoPol, a value-added fucose-rich extracellular polysaccharide.
28013138	1	55	from	industry	176:183	arg1	by-product					138:147	a by-product	136:147	a by-product from the tomato processing industry	136:183	Out-of-specification tomato paste, a by-product from the tomato processing industry, was used as the sole substrate for cultivation of the bacterium Enterobacter A47 and production of FucoPol, a value-added fucose-rich extracellular polysaccharide.
28013138	3	56	theme	groups	764:769	arg1	content					771:777	an acyl groups content	756:777	an acyl groups content of 13wt%	756:786	The polymer produced had the typical FucoPol composition (37mol% fucose, 27mol% galactose, 23mol% glucose and 12mol% glucuronic acid, with an acyl groups content of 13wt%).
28013138	0	57	theme	paste	61:65	arg1	by-product					67:76	waste tomato paste by-product	48:76	waste tomato paste by-product	48:76	Production of FucoPol by Enterobacter A47 using waste tomato paste by-product as sole carbon source.
28013138	3	58	contain	had	638:640	arg2	composition					662:672	the typical FucoPol composition	642:672	the typical FucoPol composition (37mol% fucose, 27mol% galactose, 23mol% glucose and 12mol% glucuronic acid, with an acyl groups content of 13wt%)	642:787	The polymer produced had the typical FucoPol composition (37mol% fucose, 27mol% galactose, 23mol% glucose and 12mol% glucuronic acid, with an acyl groups content of 13wt%).
28013138	3	58	contain	had	638:640	arg1	polymer					621:627	The polymer	617:627	The polymer produced	617:636	The polymer produced had the typical FucoPol composition (37mol% fucose, 27mol% galactose, 23mol% glucose and 12mol% glucuronic acid, with an acyl groups content of 13wt%).
28013138	2	59	theme	tested	370:375	arg1	strategies					387:396	the different tested fed-batch strategies	356:396	the different tested fed-batch strategies	356:396	Among the different tested fed-batch strategies, pH-stat, DO-stat and continuous substrate feeding, the highest production (8.77gL-1) and overall volumetric productivity (2.92gL-1d-1) were obtained with continuous substrate feeding at a constant flow rate of 11gh-1.
28013138	0	60	theme	tomato	54:59	arg1	by-product					67:76	waste tomato paste by-product	48:76	waste tomato paste by-product	48:76	Production of FucoPol by Enterobacter A47 using waste tomato paste by-product as sole carbon source.
28013138	1	61	theme	fucose-rich	308:318	arg1	polysaccharide					334:347	a value-added fucose-rich extracellular polysaccharide	294:347	a value-added fucose-rich extracellular polysaccharide	294:347	Out-of-specification tomato paste, a by-product from the tomato processing industry, was used as the sole substrate for cultivation of the bacterium Enterobacter A47 and production of FucoPol, a value-added fucose-rich extracellular polysaccharide.
28013138	1	61	theme	fucose-rich	308:318	arg1	substrate					207:215	the sole substrate	198:215	the sole substrate for cultivation of the bacterium Enterobacter A47 and production of FucoPol	198:291	Out-of-specification tomato paste, a by-product from the tomato processing industry, was used as the sole substrate for cultivation of the bacterium Enterobacter A47 and production of FucoPol, a value-added fucose-rich extracellular polysaccharide.
28013138	2	62	theme	substrate	431:439	arg1	feeding					441:447	continuous substrate feeding	420:447	continuous substrate feeding	420:447	Among the different tested fed-batch strategies, pH-stat, DO-stat and continuous substrate feeding, the highest production (8.77gL-1) and overall volumetric productivity (2.92gL-1d-1) were obtained with continuous substrate feeding at a constant flow rate of 11gh-1.
28013138	2	63	theme	different	360:368	arg1	strategies					387:396	the different tested fed-batch strategies	356:396	the different tested fed-batch strategies	356:396	Among the different tested fed-batch strategies, pH-stat, DO-stat and continuous substrate feeding, the highest production (8.77gL-1) and overall volumetric productivity (2.92gL-1d-1) were obtained with continuous substrate feeding at a constant flow rate of 11gh-1.
26047900	1	0	theme	evaporation	318:328	arg1	method					330:335	simple solution mixing evaporation method	295:335	simple solution mixing evaporation method	295:335	The present work reports the successful preparation, thermal and mechanical characterization of high performance films of Na(+) montmorillonite (MMT)/graphene oxide (GO)/chitosan (CS) composite using simple solution mixing evaporation method.
26047900	1	1	theme	oxide	254:258	arg1	/chitosan					264:272	montmorillonite (MMT)/graphene oxide (GO)/chitosan	223:272	montmorillonite (MMT)/graphene oxide (GO)/chitosan	223:272	The present work reports the successful preparation, thermal and mechanical characterization of high performance films of Na(+) montmorillonite (MMT)/graphene oxide (GO)/chitosan (CS) composite using simple solution mixing evaporation method.
26047900	1	2	theme	high	191:194	arg1	films					208:212	high performance films	191:212	high performance films of Na(+) montmorillonite (MMT)/graphene oxide (GO)/chitosan (CS) composite using simple solution mixing evaporation method	191:335	The present work reports the successful preparation, thermal and mechanical characterization of high performance films of Na(+) montmorillonite (MMT)/graphene oxide (GO)/chitosan (CS) composite using simple solution mixing evaporation method.
26047900	3	3	theme	thermogravimetric	596:612	arg1	TGA					624:626	TGA	624:626	TGA	624:626	The thermal stability and mechanical properties of these films were investigated by thermogravimetric analysis (TGA) and mechanical testing (Instron 8871).
26047900	3	3	theme	thermogravimetric	596:612	arg1	analysis					614:621	thermogravimetric analysis	596:621	thermogravimetric analysis (TGA)	596:627	The thermal stability and mechanical properties of these films were investigated by thermogravimetric analysis (TGA) and mechanical testing (Instron 8871).
26047900	8	4	theme	tensile	942:948	arg1	strength					950:957	The tensile strength	938:957	The tensile strength of (5 wt.	938:967	The tensile strength of (5 wt.
26047900	4	5	theme	chitosan	740:747	arg1	composites					726:735	the composites	722:735	the composites of chitosan, MMT, and graphene oxide	722:772	The results obtained from these studies revealed that the composites of chitosan, MMT, and graphene oxide were homogeneous in nature.
26047900	4	5	theme	chitosan	740:747	arg1	oxide					768:772	graphene oxide	759:772	graphene oxide	759:772	The results obtained from these studies revealed that the composites of chitosan, MMT, and graphene oxide were homogeneous in nature.
26047900	4	5	theme	chitosan	740:747	arg1	chitosan					740:747	chitosan	740:747	chitosan	740:747	The results obtained from these studies revealed that the composites of chitosan, MMT, and graphene oxide were homogeneous in nature.
26047900	4	5	theme	chitosan	740:747	arg1	MMT					750:752	MMT	750:752	MMT	750:752	The results obtained from these studies revealed that the composites of chitosan, MMT, and graphene oxide were homogeneous in nature.
26047900	4	5	theme	chitosan	740:747	arg1	homogeneous					779:789	homogeneous	779:789	homogeneous	779:789	The results obtained from these studies revealed that the composites of chitosan, MMT, and graphene oxide were homogeneous in nature.
26047900	1	6	theme	performance	196:206	arg1	films					208:212	high performance films	191:212	high performance films of Na(+) montmorillonite (MMT)/graphene oxide (GO)/chitosan (CS) composite using simple solution mixing evaporation method	191:335	The present work reports the successful preparation, thermal and mechanical characterization of high performance films of Na(+) montmorillonite (MMT)/graphene oxide (GO)/chitosan (CS) composite using simple solution mixing evaporation method.
26047900	4	7	from	nature	794:799	arg1	composites					726:735	the composites	722:735	the composites of chitosan, MMT, and graphene oxide	722:772	The results obtained from these studies revealed that the composites of chitosan, MMT, and graphene oxide were homogeneous in nature.
26047900	4	7	from	nature	794:799	arg1	oxide					768:772	graphene oxide	759:772	graphene oxide	759:772	The results obtained from these studies revealed that the composites of chitosan, MMT, and graphene oxide were homogeneous in nature.
26047900	4	7	from	nature	794:799	arg1	chitosan					740:747	chitosan	740:747	chitosan	740:747	The results obtained from these studies revealed that the composites of chitosan, MMT, and graphene oxide were homogeneous in nature.
26047900	4	7	from	nature	794:799	arg1	MMT					750:752	MMT	750:752	MMT	750:752	The results obtained from these studies revealed that the composites of chitosan, MMT, and graphene oxide were homogeneous in nature.
26047900	4	7	from	nature	794:799	arg1	homogeneous					779:789	homogeneous	779:789	homogeneous	779:789	The results obtained from these studies revealed that the composites of chitosan, MMT, and graphene oxide were homogeneous in nature.
26047900	6	8	theme	%	917:917	arg1	MMT					919:921	% MMT	917:921	% MMT	917:921	% MMT and 1 wt.
26047900	1	9	theme	present	99:105	arg1	work					107:110	The present work	95:110	The present work	95:110	The present work reports the successful preparation, thermal and mechanical characterization of high performance films of Na(+) montmorillonite (MMT)/graphene oxide (GO)/chitosan (CS) composite using simple solution mixing evaporation method.
26047900	1	10	theme	films	208:212	arg1	characterization					171:186	thermal and mechanical characterization	148:186	thermal and mechanical characterization of high performance films of Na(+) montmorillonite (MMT)/graphene oxide (GO)/chitosan (CS) composite using simple solution mixing evaporation method	148:335	The present work reports the successful preparation, thermal and mechanical characterization of high performance films of Na(+) montmorillonite (MMT)/graphene oxide (GO)/chitosan (CS) composite using simple solution mixing evaporation method.
26047900	1	10	theme	films	208:212	arg1	preparation					135:145	the successful preparation	120:145	the successful preparation	120:145	The present work reports the successful preparation, thermal and mechanical characterization of high performance films of Na(+) montmorillonite (MMT)/graphene oxide (GO)/chitosan (CS) composite using simple solution mixing evaporation method.
26047900	2	11	theme	scanning	428:435	arg1	microscopy					446:455	scanning electron microscopy	428:455	scanning electron microscopy (SEM)	428:461	The formations of films were verified by Fourier transform infrared (FT-IR) spectroscopy, scanning electron microscopy (SEM), X-ray diffraction (XRD) and Raman spectroscopy.
26047900	2	11	theme	scanning	428:435	arg1	SEM					458:460	SEM	458:460	SEM	458:460	The formations of films were verified by Fourier transform infrared (FT-IR) spectroscopy, scanning electron microscopy (SEM), X-ray diffraction (XRD) and Raman spectroscopy.
26047900	1	12	theme	/chitosan	264:272	arg1	composite					279:287	Na(+) montmorillonite (MMT)/graphene oxide (GO)/chitosan (CS) composite	217:287	Na(+) montmorillonite (MMT)/graphene oxide (GO)/chitosan (CS) composite using simple solution mixing evaporation method	217:335	The present work reports the successful preparation, thermal and mechanical characterization of high performance films of Na(+) montmorillonite (MMT)/graphene oxide (GO)/chitosan (CS) composite using simple solution mixing evaporation method.
26047900	3	13	theme	mechanical	538:547	arg1	properties					549:558	mechanical properties	538:558	mechanical properties	538:558	The thermal stability and mechanical properties of these films were investigated by thermogravimetric analysis (TGA) and mechanical testing (Instron 8871).
26047900	1	14	theme	Na	217:218	arg1	composite					279:287	Na(+) montmorillonite (MMT)/graphene oxide (GO)/chitosan (CS) composite	217:287	Na(+) montmorillonite (MMT)/graphene oxide (GO)/chitosan (CS) composite using simple solution mixing evaporation method	217:335	The present work reports the successful preparation, thermal and mechanical characterization of high performance films of Na(+) montmorillonite (MMT)/graphene oxide (GO)/chitosan (CS) composite using simple solution mixing evaporation method.
26047900	3	15	theme	thermal	516:522	arg1	stability					524:532	thermal stability	516:532	thermal stability	516:532	The thermal stability and mechanical properties of these films were investigated by thermogravimetric analysis (TGA) and mechanical testing (Instron 8871).
26047900	2	16	theme	FT-IR	407:411	arg1	spectroscopy					414:425	(FT-IR) spectroscopy	406:425	(FT-IR) spectroscopy	406:425	The formations of films were verified by Fourier transform infrared (FT-IR) spectroscopy, scanning electron microscopy (SEM), X-ray diffraction (XRD) and Raman spectroscopy.
26047900	1	17	theme	successful	124:133	arg1	characterization					171:186	thermal and mechanical characterization	148:186	thermal and mechanical characterization of high performance films of Na(+) montmorillonite (MMT)/graphene oxide (GO)/chitosan (CS) composite using simple solution mixing evaporation method	148:335	The present work reports the successful preparation, thermal and mechanical characterization of high performance films of Na(+) montmorillonite (MMT)/graphene oxide (GO)/chitosan (CS) composite using simple solution mixing evaporation method.
26047900	1	17	theme	successful	124:133	arg1	preparation					135:145	the successful preparation	120:145	the successful preparation	120:145	The present work reports the successful preparation, thermal and mechanical characterization of high performance films of Na(+) montmorillonite (MMT)/graphene oxide (GO)/chitosan (CS) composite using simple solution mixing evaporation method.
26047900	2	18	theme	films	356:360	arg1	formations					342:351	The formations	338:351	The formations of films	338:360	The formations of films were verified by Fourier transform infrared (FT-IR) spectroscopy, scanning electron microscopy (SEM), X-ray diffraction (XRD) and Raman spectroscopy.
26047900	4	19	theme	oxide	768:772	arg1	composites					726:735	the composites	722:735	the composites of chitosan, MMT, and graphene oxide	722:772	The results obtained from these studies revealed that the composites of chitosan, MMT, and graphene oxide were homogeneous in nature.
26047900	4	19	theme	oxide	768:772	arg1	oxide					768:772	graphene oxide	759:772	graphene oxide	759:772	The results obtained from these studies revealed that the composites of chitosan, MMT, and graphene oxide were homogeneous in nature.
26047900	4	19	theme	oxide	768:772	arg1	chitosan					740:747	chitosan	740:747	chitosan	740:747	The results obtained from these studies revealed that the composites of chitosan, MMT, and graphene oxide were homogeneous in nature.
26047900	4	19	theme	oxide	768:772	arg1	MMT					750:752	MMT	750:752	MMT	750:752	The results obtained from these studies revealed that the composites of chitosan, MMT, and graphene oxide were homogeneous in nature.
26047900	4	19	theme	oxide	768:772	arg1	homogeneous					779:789	homogeneous	779:789	homogeneous	779:789	The results obtained from these studies revealed that the composites of chitosan, MMT, and graphene oxide were homogeneous in nature.
26047900	5	20	theme	MMT	826:828	arg1	effect					816:821	A synergistic effect	802:821	A synergistic effect of MMT	802:828	A synergistic effect of MMT and GO reinforcing on chitosan matrix was observed for the first time, in case of 5 wt.
26047900	5	21	theme	synergistic	804:814	arg1	effect					816:821	A synergistic effect	802:821	A synergistic effect of MMT	802:828	A synergistic effect of MMT and GO reinforcing on chitosan matrix was observed for the first time, in case of 5 wt.
26047900	1	22	theme	composite	279:287	arg1	films					208:212	high performance films	191:212	high performance films of Na(+) montmorillonite (MMT)/graphene oxide (GO)/chitosan (CS) composite using simple solution mixing evaporation method	191:335	The present work reports the successful preparation, thermal and mechanical characterization of high performance films of Na(+) montmorillonite (MMT)/graphene oxide (GO)/chitosan (CS) composite using simple solution mixing evaporation method.
26047900	2	23	theme	Raman	492:496	arg1	spectroscopy					498:509	Raman spectroscopy	492:509	Raman spectroscopy	492:509	The formations of films were verified by Fourier transform infrared (FT-IR) spectroscopy, scanning electron microscopy (SEM), X-ray diffraction (XRD) and Raman spectroscopy.
26047900	2	24	dep	transform	387:395	arg1	infrared					397:404	infrared	397:404	transform infrared (FT-IR) spectroscopy, scanning electron microscopy (SEM), X-ray diffraction (XRD) and Raman spectroscopy	387:509	The formations of films were verified by Fourier transform infrared (FT-IR) spectroscopy, scanning electron microscopy (SEM), X-ray diffraction (XRD) and Raman spectroscopy.
26047900	4	25	from	homogeneous	779:789	arg1	nature					794:799	nature	794:799	nature	794:799	The results obtained from these studies revealed that the composites of chitosan, MMT, and graphene oxide were homogeneous in nature.
26047900	1	26	theme	montmorillonite	223:237	arg1	oxide					254:258	montmorillonite (MMT)/graphene oxide	223:258	montmorillonite (MMT)/graphene oxide (GO)/chitosan	223:272	The present work reports the successful preparation, thermal and mechanical characterization of high performance films of Na(+) montmorillonite (MMT)/graphene oxide (GO)/chitosan (CS) composite using simple solution mixing evaporation method.
26047900	10	27	theme	GO/CS	984:988	arg1	composite					990:998	%) GO/CS composite	981:998	%) GO/CS composite	981:998	%) GO/CS composite was formed 9±0.23% and 27±0.25% higher than that of the (1 wt.
26047900	5	28	theme	chitosan	852:859	arg1	matrix					861:866	chitosan matrix	852:866	chitosan matrix	852:866	A synergistic effect of MMT and GO reinforcing on chitosan matrix was observed for the first time, in case of 5 wt.
26047900	2	29	theme	electron	437:444	arg1	microscopy					446:455	scanning electron microscopy	428:455	scanning electron microscopy (SEM)	428:461	The formations of films were verified by Fourier transform infrared (FT-IR) spectroscopy, scanning electron microscopy (SEM), X-ray diffraction (XRD) and Raman spectroscopy.
26047900	2	29	theme	electron	437:444	arg1	SEM					458:460	SEM	458:460	SEM	458:460	The formations of films were verified by Fourier transform infrared (FT-IR) spectroscopy, scanning electron microscopy (SEM), X-ray diffraction (XRD) and Raman spectroscopy.
26047900	1	30	theme	simple	295:300	arg1	method					330:335	simple solution mixing evaporation method	295:335	simple solution mixing evaporation method	295:335	The present work reports the successful preparation, thermal and mechanical characterization of high performance films of Na(+) montmorillonite (MMT)/graphene oxide (GO)/chitosan (CS) composite using simple solution mixing evaporation method.
26047900	10	31	dep	GO/CS	984:988	arg1	%					981:981	%	981:981	%	981:981	%) GO/CS composite was formed 9±0.23% and 27±0.25% higher than that of the (1 wt.
26047900	4	32	theme	graphene	759:766	arg1	oxide					768:772	graphene oxide	759:772	graphene oxide	759:772	The results obtained from these studies revealed that the composites of chitosan, MMT, and graphene oxide were homogeneous in nature.
26047900	1	33	theme	solution	302:309	arg1	method					330:335	simple solution mixing evaporation method	295:335	simple solution mixing evaporation method	295:335	The present work reports the successful preparation, thermal and mechanical characterization of high performance films of Na(+) montmorillonite (MMT)/graphene oxide (GO)/chitosan (CS) composite using simple solution mixing evaporation method.
26047900	4	34	theme	MMT	750:752	arg1	composites					726:735	the composites	722:735	the composites of chitosan, MMT, and graphene oxide	722:772	The results obtained from these studies revealed that the composites of chitosan, MMT, and graphene oxide were homogeneous in nature.
26047900	4	34	theme	MMT	750:752	arg1	oxide					768:772	graphene oxide	759:772	graphene oxide	759:772	The results obtained from these studies revealed that the composites of chitosan, MMT, and graphene oxide were homogeneous in nature.
26047900	4	34	theme	MMT	750:752	arg1	chitosan					740:747	chitosan	740:747	chitosan	740:747	The results obtained from these studies revealed that the composites of chitosan, MMT, and graphene oxide were homogeneous in nature.
26047900	4	34	theme	MMT	750:752	arg1	MMT					750:752	MMT	750:752	MMT	750:752	The results obtained from these studies revealed that the composites of chitosan, MMT, and graphene oxide were homogeneous in nature.
26047900	4	34	theme	MMT	750:752	arg1	homogeneous					779:789	homogeneous	779:789	homogeneous	779:789	The results obtained from these studies revealed that the composites of chitosan, MMT, and graphene oxide were homogeneous in nature.
26047900	3	35	theme	films	569:573	arg1	stability					524:532	thermal stability	516:532	thermal stability	516:532	The thermal stability and mechanical properties of these films were investigated by thermogravimetric analysis (TGA) and mechanical testing (Instron 8871).
26047900	3	35	theme	films	569:573	arg1	properties					549:558	mechanical properties	538:558	mechanical properties	538:558	The thermal stability and mechanical properties of these films were investigated by thermogravimetric analysis (TGA) and mechanical testing (Instron 8871).
26047900	1	36	theme	thermal	148:154	arg1	characterization					171:186	thermal and mechanical characterization	148:186	thermal and mechanical characterization of high performance films of Na(+) montmorillonite (MMT)/graphene oxide (GO)/chitosan (CS) composite using simple solution mixing evaporation method	148:335	The present work reports the successful preparation, thermal and mechanical characterization of high performance films of Na(+) montmorillonite (MMT)/graphene oxide (GO)/chitosan (CS) composite using simple solution mixing evaporation method.
26047900	1	36	theme	thermal	148:154	arg1	preparation					135:145	the successful preparation	120:145	the successful preparation	120:145	The present work reports the successful preparation, thermal and mechanical characterization of high performance films of Na(+) montmorillonite (MMT)/graphene oxide (GO)/chitosan (CS) composite using simple solution mixing evaporation method.
26047900	5	37	theme	first	889:893	arg1	time					895:898	the first time	885:898	the first time	885:898	A synergistic effect of MMT and GO reinforcing on chitosan matrix was observed for the first time, in case of 5 wt.
26047900	1	38	dep	oxide	254:258	arg1	GO					261:262	GO	261:262	GO	261:262	The present work reports the successful preparation, thermal and mechanical characterization of high performance films of Na(+) montmorillonite (MMT)/graphene oxide (GO)/chitosan (CS) composite using simple solution mixing evaporation method.
26047900	0	39	dep	composite	40:48	arg1	properties					83:92	properties	83:92	properties	83:92	Montmorillonite/graphene oxide/chitosan composite: Synthesis, characterization and properties.
26047900	0	39	dep	composite	40:48	arg1	characterization					62:77	characterization	62:77	characterization	62:77	Montmorillonite/graphene oxide/chitosan composite: Synthesis, characterization and properties.
26047900	0	39	dep	composite	40:48	arg1	Synthesis					51:59	Synthesis	51:59	Synthesis	51:59	Montmorillonite/graphene oxide/chitosan composite: Synthesis, characterization and properties.
26047900	8	40	dep	wt	965:966	arg1	5					963:963	5	963:963	5	963:963	The tensile strength of (5 wt.
26047900	1	41	theme	MMT	240:242	arg1	oxide					254:258	montmorillonite (MMT)/graphene oxide	223:258	montmorillonite (MMT)/graphene oxide (GO)/chitosan	223:272	The present work reports the successful preparation, thermal and mechanical characterization of high performance films of Na(+) montmorillonite (MMT)/graphene oxide (GO)/chitosan (CS) composite using simple solution mixing evaporation method.
26047900	3	42	theme	mechanical	633:642	arg1	Instron					653:659	Instron	653:659	Instron	653:659	The thermal stability and mechanical properties of these films were investigated by thermogravimetric analysis (TGA) and mechanical testing (Instron 8871).
26047900	3	42	theme	mechanical	633:642	arg1	testing					644:650	mechanical testing	633:650	mechanical testing (Instron 8871)	633:665	The thermal stability and mechanical properties of these films were investigated by thermogravimetric analysis (TGA) and mechanical testing (Instron 8871).
26047900	2	43	dep	Fourier	379:385	arg1	transform					387:395	transform	387:395	transform infrared (FT-IR) spectroscopy, scanning electron microscopy (SEM), X-ray diffraction (XRD) and Raman spectroscopy	387:509	The formations of films were verified by Fourier transform infrared (FT-IR) spectroscopy, scanning electron microscopy (SEM), X-ray diffraction (XRD) and Raman spectroscopy.
26047900	1	44	theme	mechanical	160:169	arg1	characterization					171:186	thermal and mechanical characterization	148:186	thermal and mechanical characterization of high performance films of Na(+) montmorillonite (MMT)/graphene oxide (GO)/chitosan (CS) composite using simple solution mixing evaporation method	148:335	The present work reports the successful preparation, thermal and mechanical characterization of high performance films of Na(+) montmorillonite (MMT)/graphene oxide (GO)/chitosan (CS) composite using simple solution mixing evaporation method.
26047900	1	44	theme	mechanical	160:169	arg1	preparation					135:145	the successful preparation	120:145	the successful preparation	120:145	The present work reports the successful preparation, thermal and mechanical characterization of high performance films of Na(+) montmorillonite (MMT)/graphene oxide (GO)/chitosan (CS) composite using simple solution mixing evaporation method.
26047900	2	45	theme	X-ray	464:468	arg1	XRD					483:485	XRD	483:485	XRD	483:485	The formations of films were verified by Fourier transform infrared (FT-IR) spectroscopy, scanning electron microscopy (SEM), X-ray diffraction (XRD) and Raman spectroscopy.
26047900	2	45	theme	X-ray	464:468	arg1	diffraction					470:480	X-ray diffraction	464:480	X-ray diffraction (XRD)	464:486	The formations of films were verified by Fourier transform infrared (FT-IR) spectroscopy, scanning electron microscopy (SEM), X-ray diffraction (XRD) and Raman spectroscopy.
26047900	1	46	theme	mixing	311:316	arg1	method					330:335	simple solution mixing evaporation method	295:335	simple solution mixing evaporation method	295:335	The present work reports the successful preparation, thermal and mechanical characterization of high performance films of Na(+) montmorillonite (MMT)/graphene oxide (GO)/chitosan (CS) composite using simple solution mixing evaporation method.
26047900	3	47	dep	stability	524:532	arg1	The					512:514	The	512:514	The	512:514	The thermal stability and mechanical properties of these films were investigated by thermogravimetric analysis (TGA) and mechanical testing (Instron 8871).
26047900	8	48	theme	wt	965:966	arg1	strength					950:957	The tensile strength	938:957	The tensile strength of (5 wt.	938:967	The tensile strength of (5 wt.
26047900	1	49	theme	/graphene	244:252	arg1	oxide					254:258	montmorillonite (MMT)/graphene oxide	223:258	montmorillonite (MMT)/graphene oxide (GO)/chitosan	223:272	The present work reports the successful preparation, thermal and mechanical characterization of high performance films of Na(+) montmorillonite (MMT)/graphene oxide (GO)/chitosan (CS) composite using simple solution mixing evaporation method.
24980061	2	0	theme	polymeric	410:418	arg1	networks					420:427	hydrophilic polymeric networks	398:427	hydrophilic polymeric networks due to their non-immunogenic and non-antigenic properties	398:485	Naturally derived polysaccharides, like pullulan and dextran, are attractive materials with which to form hydrophilic polymeric networks due to their non-immunogenic and non-antigenic properties.
24980061	7	1	theme	composite	1153:1161	arg1	scaffold					1163:1170	the composite scaffold	1149:1170	the composite scaffold	1149:1170	Lastly, we showed that the VEGF released from the composite scaffold retained its capacity to stimulate endothelial cell growth.
24980061	2	2	link	derived	302:308	arg1	polysaccharides					310:324	Naturally derived polysaccharides	292:324	Naturally derived polysaccharides	292:324	Naturally derived polysaccharides, like pullulan and dextran, are attractive materials with which to form hydrophilic polymeric networks due to their non-immunogenic and non-antigenic properties.
24980061	2	2	link	derived	302:308	arg1	materials					369:377	attractive materials	358:377	attractive materials with which to form hydrophilic polymeric networks due to their non-immunogenic and non-antigenic properties	358:485	Naturally derived polysaccharides, like pullulan and dextran, are attractive materials with which to form hydrophilic polymeric networks due to their non-immunogenic and non-antigenic properties.
24980061	4	3	theme	pullulan-dextran	585:600	arg1	scaffolds					602:610	pullulan-dextran scaffolds	585:610	pullulan-dextran scaffolds with interfacial polyelectrolyte complexation (IPC) fibers	585:669	In this study, we modified pullulan-dextran scaffolds with interfacial polyelectrolyte complexation (IPC) fibers to improve their ability to support adherent cell growth.
24980061	0	4	with	platform	107:114	arg1	distribution					159:170	spatial distribution	151:170	spatial distribution	151:170	Composite pullulan-dextran polysaccharide scaffold with interfacial polyelectrolyte complexation fibers: a platform with enhanced cell interaction and spatial distribution.
24980061	0	4	with	platform	107:114	arg1	interaction					135:145	enhanced cell interaction	121:145	enhanced cell interaction	121:145	Composite pullulan-dextran polysaccharide scaffold with interfacial polyelectrolyte complexation fibers: a platform with enhanced cell interaction and spatial distribution.
24980061	2	5	theme	hydrophilic	398:408	arg1	networks					420:427	hydrophilic polymeric networks	398:427	hydrophilic polymeric networks due to their non-immunogenic and non-antigenic properties	398:485	Naturally derived polysaccharides, like pullulan and dextran, are attractive materials with which to form hydrophilic polymeric networks due to their non-immunogenic and non-antigenic properties.
24980061	8	6	from	incorporation	1236:1248	arg1	scaffold					1297:1304	the pullulan-dextran hydrogel scaffold	1267:1304	the pullulan-dextran hydrogel scaffold	1267:1304	The incorporation of IPC fibers in the pullulan-dextran hydrogel scaffold improved its functionality and biological activity, thus enhancing its potential in tissue engineering applications.
24980061	0	7	theme	enhanced	121:128	arg1	interaction					135:145	enhanced cell interaction	121:145	enhanced cell interaction	121:145	Composite pullulan-dextran polysaccharide scaffold with interfacial polyelectrolyte complexation fibers: a platform with enhanced cell interaction and spatial distribution.
24980061	6	8	theme	endothelial	1030:1040	arg1	biologics					986:994	the biologics bovine serum albumin and vascular endothelial growth factor (VEGF)	982:1061	the biologics bovine serum albumin and vascular endothelial growth factor (VEGF) incorporated in the composite scaffold	982:1100	We also demonstrated the zero-order release kinetics of the biologics bovine serum albumin and vascular endothelial growth factor (VEGF) incorporated in the composite scaffold.
24980061	6	8	theme	endothelial	1030:1040	arg1	VEGF					1057:1060	VEGF	1057:1060	VEGF	1057:1060	We also demonstrated the zero-order release kinetics of the biologics bovine serum albumin and vascular endothelial growth factor (VEGF) incorporated in the composite scaffold.
24980061	6	8	theme	endothelial	1030:1040	arg1	factor					1049:1054	vascular endothelial growth factor	1021:1054	vascular endothelial growth factor (VEGF)	1021:1061	We also demonstrated the zero-order release kinetics of the biologics bovine serum albumin and vascular endothelial growth factor (VEGF) incorporated in the composite scaffold.
24980061	8	9	theme	hydrogel	1288:1295	arg1	scaffold					1297:1304	the pullulan-dextran hydrogel scaffold	1267:1304	the pullulan-dextran hydrogel scaffold	1267:1304	The incorporation of IPC fibers in the pullulan-dextran hydrogel scaffold improved its functionality and biological activity, thus enhancing its potential in tissue engineering applications.
24980061	8	10	theme	pullulan-dextran	1271:1286	arg1	scaffold					1297:1304	the pullulan-dextran hydrogel scaffold	1267:1304	the pullulan-dextran hydrogel scaffold	1267:1304	The incorporation of IPC fibers in the pullulan-dextran hydrogel scaffold improved its functionality and biological activity, thus enhancing its potential in tissue engineering applications.
24980061	6	11	theme	vascular	1021:1028	arg1	biologics					986:994	the biologics bovine serum albumin and vascular endothelial growth factor (VEGF)	982:1061	the biologics bovine serum albumin and vascular endothelial growth factor (VEGF) incorporated in the composite scaffold	982:1100	We also demonstrated the zero-order release kinetics of the biologics bovine serum albumin and vascular endothelial growth factor (VEGF) incorporated in the composite scaffold.
24980061	6	11	theme	vascular	1021:1028	arg1	VEGF					1057:1060	VEGF	1057:1060	VEGF	1057:1060	We also demonstrated the zero-order release kinetics of the biologics bovine serum albumin and vascular endothelial growth factor (VEGF) incorporated in the composite scaffold.
24980061	6	11	theme	vascular	1021:1028	arg1	factor					1049:1054	vascular endothelial growth factor	1021:1054	vascular endothelial growth factor (VEGF)	1021:1061	We also demonstrated the zero-order release kinetics of the biologics bovine serum albumin and vascular endothelial growth factor (VEGF) incorporated in the composite scaffold.
24980061	5	12	theme	pullulan-dextran-IPC	748:767	arg1	laden					794:798	the pullulan-dextran-IPC fiber composite scaffold laden	744:798	the pullulan-dextran-IPC fiber composite scaffold laden with extracellular matrix protein	744:832	We showed that the pullulan-dextran-IPC fiber composite scaffold laden with extracellular matrix protein has improved cell adhesion and proliferation compared to the plain polysaccharide scaffold.
24980061	5	13	theme	cell	847:850	arg1	adhesion					852:859	cell adhesion	847:859	cell adhesion	847:859	We showed that the pullulan-dextran-IPC fiber composite scaffold laden with extracellular matrix protein has improved cell adhesion and proliferation compared to the plain polysaccharide scaffold.
24980061	6	14	dep	biologics	986:994	arg1	biologics					986:994	the biologics bovine serum albumin and vascular endothelial growth factor (VEGF)	982:1061	the biologics bovine serum albumin and vascular endothelial growth factor (VEGF) incorporated in the composite scaffold	982:1100	We also demonstrated the zero-order release kinetics of the biologics bovine serum albumin and vascular endothelial growth factor (VEGF) incorporated in the composite scaffold.
24980061	6	14	dep	biologics	986:994	arg1	VEGF					1057:1060	VEGF	1057:1060	VEGF	1057:1060	We also demonstrated the zero-order release kinetics of the biologics bovine serum albumin and vascular endothelial growth factor (VEGF) incorporated in the composite scaffold.
24980061	6	14	dep	biologics	986:994	arg1	factor					1049:1054	vascular endothelial growth factor	1021:1054	vascular endothelial growth factor (VEGF)	1021:1061	We also demonstrated the zero-order release kinetics of the biologics bovine serum albumin and vascular endothelial growth factor (VEGF) incorporated in the composite scaffold.
24980061	6	14	dep	biologics	986:994	arg1	albumin					1009:1015	bovine serum albumin	996:1015	bovine serum albumin	996:1015	We also demonstrated the zero-order release kinetics of the biologics bovine serum albumin and vascular endothelial growth factor (VEGF) incorporated in the composite scaffold.
24980061	8	15	theme	engineering	1397:1407	arg1	applications					1409:1420	tissue engineering applications	1390:1420	tissue engineering applications	1390:1420	The incorporation of IPC fibers in the pullulan-dextran hydrogel scaffold improved its functionality and biological activity, thus enhancing its potential in tissue engineering applications.
24980061	8	16	theme	tissue	1390:1395	arg1	applications					1409:1420	tissue engineering applications	1390:1420	tissue engineering applications	1390:1420	The incorporation of IPC fibers in the pullulan-dextran hydrogel scaffold improved its functionality and biological activity, thus enhancing its potential in tissue engineering applications.
24980061	3	17	theme	inherent	503:510	arg1	hydrophilicity					512:525	their inherent hydrophilicity	497:525	their inherent hydrophilicity	497:525	However, their inherent hydrophilicity prevents adherent cell growth.
24980061	0	18	theme	cell	130:133	arg1	interaction					135:145	enhanced cell interaction	121:145	enhanced cell interaction	121:145	Composite pullulan-dextran polysaccharide scaffold with interfacial polyelectrolyte complexation fibers: a platform with enhanced cell interaction and spatial distribution.
24980061	2	19	theme	non-immunogenic	442:456	arg1	properties					476:485	their non-immunogenic and non-antigenic properties	436:485	their non-immunogenic and non-antigenic properties	436:485	Naturally derived polysaccharides, like pullulan and dextran, are attractive materials with which to form hydrophilic polymeric networks due to their non-immunogenic and non-antigenic properties.
24980061	0	20	theme	pullulan-dextran	10:25	arg1	scaffold					42:49	Composite pullulan-dextran polysaccharide scaffold	0:49	Composite pullulan-dextran polysaccharide scaffold with interfacial polyelectrolyte complexation fibers: a platform with enhanced cell interaction and spatial distribution.	0:171	Composite pullulan-dextran polysaccharide scaffold with interfacial polyelectrolyte complexation fibers: a platform with enhanced cell interaction and spatial distribution.
24980061	6	21	theme	serum	1003:1007	arg1	biologics					986:994	the biologics bovine serum albumin and vascular endothelial growth factor (VEGF)	982:1061	the biologics bovine serum albumin and vascular endothelial growth factor (VEGF) incorporated in the composite scaffold	982:1100	We also demonstrated the zero-order release kinetics of the biologics bovine serum albumin and vascular endothelial growth factor (VEGF) incorporated in the composite scaffold.
24980061	6	21	theme	serum	1003:1007	arg1	albumin					1009:1015	bovine serum albumin	996:1015	bovine serum albumin	996:1015	We also demonstrated the zero-order release kinetics of the biologics bovine serum albumin and vascular endothelial growth factor (VEGF) incorporated in the composite scaffold.
24980061	1	22	theme	tissue	212:217	arg1	engineering					219:229	soft tissue engineering	207:229	soft tissue engineering	207:229	Hydrogels are highly preferred in soft tissue engineering because they recapitulate the hydrated extracellular matrix.
24980061	0	23	theme	Composite	0:8	arg1	scaffold					42:49	Composite pullulan-dextran polysaccharide scaffold	0:49	Composite pullulan-dextran polysaccharide scaffold with interfacial polyelectrolyte complexation fibers: a platform with enhanced cell interaction and spatial distribution.	0:171	Composite pullulan-dextran polysaccharide scaffold with interfacial polyelectrolyte complexation fibers: a platform with enhanced cell interaction and spatial distribution.
24980061	7	24	theme	endothelial	1207:1217	arg1	growth					1224:1229	endothelial cell growth	1207:1229	endothelial cell growth	1207:1229	Lastly, we showed that the VEGF released from the composite scaffold retained its capacity to stimulate endothelial cell growth.
24980061	2	25	theme	attractive	358:367	arg1	polysaccharides					310:324	Naturally derived polysaccharides	292:324	Naturally derived polysaccharides	292:324	Naturally derived polysaccharides, like pullulan and dextran, are attractive materials with which to form hydrophilic polymeric networks due to their non-immunogenic and non-antigenic properties.
24980061	2	25	theme	attractive	358:367	arg1	materials					369:377	attractive materials	358:377	attractive materials with which to form hydrophilic polymeric networks due to their non-immunogenic and non-antigenic properties	358:485	Naturally derived polysaccharides, like pullulan and dextran, are attractive materials with which to form hydrophilic polymeric networks due to their non-immunogenic and non-antigenic properties.
24980061	8	26	theme	biological	1337:1346	arg1	activity					1348:1355	biological activity	1337:1355	biological activity	1337:1355	The incorporation of IPC fibers in the pullulan-dextran hydrogel scaffold improved its functionality and biological activity, thus enhancing its potential in tissue engineering applications.
24980061	4	27	theme	complexation	645:656	arg1	fibers					664:669	interfacial polyelectrolyte complexation (IPC) fibers	617:669	interfacial polyelectrolyte complexation (IPC) fibers	617:669	In this study, we modified pullulan-dextran scaffolds with interfacial polyelectrolyte complexation (IPC) fibers to improve their ability to support adherent cell growth.
24980061	0	28	theme	spatial	151:157	arg1	distribution					159:170	spatial distribution	151:170	spatial distribution	151:170	Composite pullulan-dextran polysaccharide scaffold with interfacial polyelectrolyte complexation fibers: a platform with enhanced cell interaction and spatial distribution.
24980061	3	29	theme	cell	545:548	arg1	growth					550:555	adherent cell growth	536:555	adherent cell growth	536:555	However, their inherent hydrophilicity prevents adherent cell growth.
24980061	0	30	theme	polysaccharide	27:40	arg1	scaffold					42:49	Composite pullulan-dextran polysaccharide scaffold	0:49	Composite pullulan-dextran polysaccharide scaffold with interfacial polyelectrolyte complexation fibers: a platform with enhanced cell interaction and spatial distribution.	0:171	Composite pullulan-dextran polysaccharide scaffold with interfacial polyelectrolyte complexation fibers: a platform with enhanced cell interaction and spatial distribution.
24980061	6	31	theme	composite	1083:1091	arg1	scaffold					1093:1100	the composite scaffold	1079:1100	the composite scaffold	1079:1100	We also demonstrated the zero-order release kinetics of the biologics bovine serum albumin and vascular endothelial growth factor (VEGF) incorporated in the composite scaffold.
24980061	5	32	theme	extracellular	805:817	arg1	protein					826:832	extracellular matrix protein	805:832	extracellular matrix protein	805:832	We showed that the pullulan-dextran-IPC fiber composite scaffold laden with extracellular matrix protein has improved cell adhesion and proliferation compared to the plain polysaccharide scaffold.
24980061	6	33	theme	bovine	996:1001	arg1	biologics					986:994	the biologics bovine serum albumin and vascular endothelial growth factor (VEGF)	982:1061	the biologics bovine serum albumin and vascular endothelial growth factor (VEGF) incorporated in the composite scaffold	982:1100	We also demonstrated the zero-order release kinetics of the biologics bovine serum albumin and vascular endothelial growth factor (VEGF) incorporated in the composite scaffold.
24980061	6	33	theme	bovine	996:1001	arg1	albumin					1009:1015	bovine serum albumin	996:1015	bovine serum albumin	996:1015	We also demonstrated the zero-order release kinetics of the biologics bovine serum albumin and vascular endothelial growth factor (VEGF) incorporated in the composite scaffold.
24980061	4	34	with	scaffolds	602:610	arg1	fibers					664:669	interfacial polyelectrolyte complexation (IPC) fibers	617:669	interfacial polyelectrolyte complexation (IPC) fibers	617:669	In this study, we modified pullulan-dextran scaffolds with interfacial polyelectrolyte complexation (IPC) fibers to improve their ability to support adherent cell growth.
24980061	4	35	theme	cell	716:719	arg1	growth					721:726	adherent cell growth	707:726	adherent cell growth	707:726	In this study, we modified pullulan-dextran scaffolds with interfacial polyelectrolyte complexation (IPC) fibers to improve their ability to support adherent cell growth.
24980061	0	36	theme	interfacial	56:66	arg1	fibers					97:102	interfacial polyelectrolyte complexation fibers	56:102	interfacial polyelectrolyte complexation fibers	56:102	Composite pullulan-dextran polysaccharide scaffold with interfacial polyelectrolyte complexation fibers: a platform with enhanced cell interaction and spatial distribution.
24980061	5	37	theme	matrix	819:824	arg1	protein					826:832	extracellular matrix protein	805:832	extracellular matrix protein	805:832	We showed that the pullulan-dextran-IPC fiber composite scaffold laden with extracellular matrix protein has improved cell adhesion and proliferation compared to the plain polysaccharide scaffold.
24980061	6	38	theme	biologics	986:994	arg1	kinetics					970:977	the zero-order release kinetics	947:977	the zero-order release kinetics of the biologics bovine serum albumin and vascular endothelial growth factor (VEGF) incorporated in the composite scaffold	947:1100	We also demonstrated the zero-order release kinetics of the biologics bovine serum albumin and vascular endothelial growth factor (VEGF) incorporated in the composite scaffold.
24980061	5	39	theme	plain	895:899	arg1	scaffold					916:923	the plain polysaccharide scaffold	891:923	the plain polysaccharide scaffold	891:923	We showed that the pullulan-dextran-IPC fiber composite scaffold laden with extracellular matrix protein has improved cell adhesion and proliferation compared to the plain polysaccharide scaffold.
24980061	5	40	with	laden	794:798	arg1	protein					826:832	extracellular matrix protein	805:832	extracellular matrix protein	805:832	We showed that the pullulan-dextran-IPC fiber composite scaffold laden with extracellular matrix protein has improved cell adhesion and proliferation compared to the plain polysaccharide scaffold.
24980061	0	41	with	scaffold	42:49	arg1	fibers					97:102	interfacial polyelectrolyte complexation fibers	56:102	interfacial polyelectrolyte complexation fibers	56:102	Composite pullulan-dextran polysaccharide scaffold with interfacial polyelectrolyte complexation fibers: a platform with enhanced cell interaction and spatial distribution.
24980061	2	42	theme	non-antigenic	462:474	arg1	properties					476:485	their non-immunogenic and non-antigenic properties	436:485	their non-immunogenic and non-antigenic properties	436:485	Naturally derived polysaccharides, like pullulan and dextran, are attractive materials with which to form hydrophilic polymeric networks due to their non-immunogenic and non-antigenic properties.
24980061	0	43	theme	complexation	84:95	arg1	fibers					97:102	interfacial polyelectrolyte complexation fibers	56:102	interfacial polyelectrolyte complexation fibers	56:102	Composite pullulan-dextran polysaccharide scaffold with interfacial polyelectrolyte complexation fibers: a platform with enhanced cell interaction and spatial distribution.
24980061	7	44	attach	released	1135:1142	arg2	VEGF					1130:1133	the VEGF	1126:1133	the VEGF released from the composite scaffold	1126:1170	Lastly, we showed that the VEGF released from the composite scaffold retained its capacity to stimulate endothelial cell growth.
24980061	7	44	attach	released	1135:1142	arg1	scaffold					1163:1170	the composite scaffold	1149:1170	the composite scaffold	1149:1170	Lastly, we showed that the VEGF released from the composite scaffold retained its capacity to stimulate endothelial cell growth.
24980061	5	45	theme	fiber	769:773	arg1	laden					794:798	the pullulan-dextran-IPC fiber composite scaffold laden	744:798	the pullulan-dextran-IPC fiber composite scaffold laden with extracellular matrix protein	744:832	We showed that the pullulan-dextran-IPC fiber composite scaffold laden with extracellular matrix protein has improved cell adhesion and proliferation compared to the plain polysaccharide scaffold.
24980061	8	46	theme	IPC	1253:1255	arg1	fibers					1257:1262	IPC fibers	1253:1262	IPC fibers	1253:1262	The incorporation of IPC fibers in the pullulan-dextran hydrogel scaffold improved its functionality and biological activity, thus enhancing its potential in tissue engineering applications.
24980061	0	47	theme	polyelectrolyte	68:82	arg1	fibers					97:102	interfacial polyelectrolyte complexation fibers	56:102	interfacial polyelectrolyte complexation fibers	56:102	Composite pullulan-dextran polysaccharide scaffold with interfacial polyelectrolyte complexation fibers: a platform with enhanced cell interaction and spatial distribution.
24980061	4	48	theme	polyelectrolyte	629:643	arg1	IPC					659:661	IPC	659:661	IPC	659:661	In this study, we modified pullulan-dextran scaffolds with interfacial polyelectrolyte complexation (IPC) fibers to improve their ability to support adherent cell growth.
24980061	4	48	theme	polyelectrolyte	629:643	arg1	complexation					645:656	interfacial polyelectrolyte complexation	617:656	interfacial polyelectrolyte complexation (IPC) fibers	617:669	In this study, we modified pullulan-dextran scaffolds with interfacial polyelectrolyte complexation (IPC) fibers to improve their ability to support adherent cell growth.
24980061	5	49	theme	composite	775:783	arg1	laden					794:798	the pullulan-dextran-IPC fiber composite scaffold laden	744:798	the pullulan-dextran-IPC fiber composite scaffold laden with extracellular matrix protein	744:832	We showed that the pullulan-dextran-IPC fiber composite scaffold laden with extracellular matrix protein has improved cell adhesion and proliferation compared to the plain polysaccharide scaffold.
24980061	6	50	theme	growth	1042:1047	arg1	biologics					986:994	the biologics bovine serum albumin and vascular endothelial growth factor (VEGF)	982:1061	the biologics bovine serum albumin and vascular endothelial growth factor (VEGF) incorporated in the composite scaffold	982:1100	We also demonstrated the zero-order release kinetics of the biologics bovine serum albumin and vascular endothelial growth factor (VEGF) incorporated in the composite scaffold.
24980061	6	50	theme	growth	1042:1047	arg1	VEGF					1057:1060	VEGF	1057:1060	VEGF	1057:1060	We also demonstrated the zero-order release kinetics of the biologics bovine serum albumin and vascular endothelial growth factor (VEGF) incorporated in the composite scaffold.
24980061	6	50	theme	growth	1042:1047	arg1	factor					1049:1054	vascular endothelial growth factor	1021:1054	vascular endothelial growth factor (VEGF)	1021:1061	We also demonstrated the zero-order release kinetics of the biologics bovine serum albumin and vascular endothelial growth factor (VEGF) incorporated in the composite scaffold.
24980061	8	51	from	potential	1377:1385	arg1	applications					1409:1420	tissue engineering applications	1390:1420	tissue engineering applications	1390:1420	The incorporation of IPC fibers in the pullulan-dextran hydrogel scaffold improved its functionality and biological activity, thus enhancing its potential in tissue engineering applications.
24980061	4	52	theme	interfacial	617:627	arg1	IPC					659:661	IPC	659:661	IPC	659:661	In this study, we modified pullulan-dextran scaffolds with interfacial polyelectrolyte complexation (IPC) fibers to improve their ability to support adherent cell growth.
24980061	4	52	theme	interfacial	617:627	arg1	complexation					645:656	interfacial polyelectrolyte complexation	617:656	interfacial polyelectrolyte complexation (IPC) fibers	617:669	In this study, we modified pullulan-dextran scaffolds with interfacial polyelectrolyte complexation (IPC) fibers to improve their ability to support adherent cell growth.
24980061	5	53	theme	scaffold	785:792	arg1	laden					794:798	the pullulan-dextran-IPC fiber composite scaffold laden	744:798	the pullulan-dextran-IPC fiber composite scaffold laden with extracellular matrix protein	744:832	We showed that the pullulan-dextran-IPC fiber composite scaffold laden with extracellular matrix protein has improved cell adhesion and proliferation compared to the plain polysaccharide scaffold.
24980061	6	54	theme	release	962:968	arg1	kinetics					970:977	the zero-order release kinetics	947:977	the zero-order release kinetics of the biologics bovine serum albumin and vascular endothelial growth factor (VEGF) incorporated in the composite scaffold	947:1100	We also demonstrated the zero-order release kinetics of the biologics bovine serum albumin and vascular endothelial growth factor (VEGF) incorporated in the composite scaffold.
24980061	0	55	dep	scaffold	42:49	arg1	platform					107:114	a platform	105:114	Composite pullulan-dextran polysaccharide scaffold with interfacial polyelectrolyte complexation fibers: a platform with enhanced cell interaction and spatial distribution.	0:171	Composite pullulan-dextran polysaccharide scaffold with interfacial polyelectrolyte complexation fibers: a platform with enhanced cell interaction and spatial distribution.
24980061	7	56	theme	cell	1219:1222	arg1	growth					1224:1229	endothelial cell growth	1207:1229	endothelial cell growth	1207:1229	Lastly, we showed that the VEGF released from the composite scaffold retained its capacity to stimulate endothelial cell growth.
24980061	1	57	theme	hydrated	261:268	arg1	matrix					284:289	the hydrated extracellular matrix	257:289	the hydrated extracellular matrix	257:289	Hydrogels are highly preferred in soft tissue engineering because they recapitulate the hydrated extracellular matrix.
24980061	6	58	theme	zero-order	951:960	arg1	kinetics					970:977	the zero-order release kinetics	947:977	the zero-order release kinetics of the biologics bovine serum albumin and vascular endothelial growth factor (VEGF) incorporated in the composite scaffold	947:1100	We also demonstrated the zero-order release kinetics of the biologics bovine serum albumin and vascular endothelial growth factor (VEGF) incorporated in the composite scaffold.
24980061	1	59	theme	extracellular	270:282	arg1	matrix					284:289	the hydrated extracellular matrix	257:289	the hydrated extracellular matrix	257:289	Hydrogels are highly preferred in soft tissue engineering because they recapitulate the hydrated extracellular matrix.
24980061	8	60	theme	fibers	1257:1262	arg1	incorporation					1236:1248	The incorporation	1232:1248	The incorporation of IPC fibers in the pullulan-dextran hydrogel scaffold	1232:1304	The incorporation of IPC fibers in the pullulan-dextran hydrogel scaffold improved its functionality and biological activity, thus enhancing its potential in tissue engineering applications.
24980061	3	61	theme	adherent	536:543	arg1	growth					550:555	adherent cell growth	536:555	adherent cell growth	536:555	However, their inherent hydrophilicity prevents adherent cell growth.
24980061	2	62	theme	derived	302:308	arg1	polysaccharides					310:324	Naturally derived polysaccharides	292:324	Naturally derived polysaccharides	292:324	Naturally derived polysaccharides, like pullulan and dextran, are attractive materials with which to form hydrophilic polymeric networks due to their non-immunogenic and non-antigenic properties.
24980061	2	62	theme	derived	302:308	arg1	materials					369:377	attractive materials	358:377	attractive materials with which to form hydrophilic polymeric networks due to their non-immunogenic and non-antigenic properties	358:485	Naturally derived polysaccharides, like pullulan and dextran, are attractive materials with which to form hydrophilic polymeric networks due to their non-immunogenic and non-antigenic properties.
24980061	2	63	theme	due	429:431	arg1	networks					420:427	hydrophilic polymeric networks	398:427	hydrophilic polymeric networks due to their non-immunogenic and non-antigenic properties	398:485	Naturally derived polysaccharides, like pullulan and dextran, are attractive materials with which to form hydrophilic polymeric networks due to their non-immunogenic and non-antigenic properties.
24980061	1	64	theme	soft	207:210	arg1	engineering					219:229	soft tissue engineering	207:229	soft tissue engineering	207:229	Hydrogels are highly preferred in soft tissue engineering because they recapitulate the hydrated extracellular matrix.
24980061	4	65	theme	adherent	707:714	arg1	growth					721:726	adherent cell growth	707:726	adherent cell growth	707:726	In this study, we modified pullulan-dextran scaffolds with interfacial polyelectrolyte complexation (IPC) fibers to improve their ability to support adherent cell growth.
24980061	5	66	theme	polysaccharide	901:914	arg1	scaffold					916:923	the plain polysaccharide scaffold	891:923	the plain polysaccharide scaffold	891:923	We showed that the pullulan-dextran-IPC fiber composite scaffold laden with extracellular matrix protein has improved cell adhesion and proliferation compared to the plain polysaccharide scaffold.
24203345	8	0	theme	tangshen	1398:1405	arg1	roots					1386:1390	the roots	1382:1390	the roots of C. tangshen	1382:1405	modesta, while 3 and 2 had relatively high contents in the roots of C. tangshen.
24203345	1	1	theme	cordifolioidyne	497:511	arg1	4					516:516	4	516:516	4	516:516	A comparative study of 56 specimens of three medicinally-used Codonopsis taxa collected from China and 54 commercial samples of Codonopsis Radix available in Chinese, Japanese and Korean markets was carried out by quantitative analysis of seven major components: codonopyrrolidium B (1), codonopyrrolidium A (2), tangshenoside I (3), cordifolioidyne B (4), lobetyolinin (5), lobetyolin (6) and lobetyol (7).
24203345	1	1	theme	cordifolioidyne	497:511	arg1	B					513:513	cordifolioidyne B	497:513	cordifolioidyne B (4)	497:517	A comparative study of 56 specimens of three medicinally-used Codonopsis taxa collected from China and 54 commercial samples of Codonopsis Radix available in Chinese, Japanese and Korean markets was carried out by quantitative analysis of seven major components: codonopyrrolidium B (1), codonopyrrolidium A (2), tangshenoside I (3), cordifolioidyne B (4), lobetyolinin (5), lobetyolin (6) and lobetyol (7).
24203345	1	1	theme	cordifolioidyne	497:511	arg1	components					414:423	seven major components	402:423	seven major components: codonopyrrolidium B (1)	402:448	A comparative study of 56 specimens of three medicinally-used Codonopsis taxa collected from China and 54 commercial samples of Codonopsis Radix available in Chinese, Japanese and Korean markets was carried out by quantitative analysis of seven major components: codonopyrrolidium B (1), codonopyrrolidium A (2), tangshenoside I (3), cordifolioidyne B (4), lobetyolinin (5), lobetyolin (6) and lobetyol (7).
24203345	1	2	theme	comparative	165:175	arg1	study					177:181	A comparative study	163:181	A comparative study of 56 specimens of three medicinally-used Codonopsis taxa collected from China and 54 commercial samples of Codonopsis Radix available in Chinese, Japanese and Korean markets	163:356	A comparative study of 56 specimens of three medicinally-used Codonopsis taxa collected from China and 54 commercial samples of Codonopsis Radix available in Chinese, Japanese and Korean markets was carried out by quantitative analysis of seven major components: codonopyrrolidium B (1), codonopyrrolidium A (2), tangshenoside I (3), cordifolioidyne B (4), lobetyolinin (5), lobetyolin (6) and lobetyol (7).
24203345	9	3	from	var	1518:1520	arg1	tangshen					1476:1483	C. tangshen	1473:1483	C. tangshen from C. pilosula and C. pilosula var	1473:1520	Therefore, 3, 2 and 1 could be chemical markers to differentiate C. tangshen from C. pilosula and C. pilosula var.
24203345	6	4	theme	other	1168:1172	arg1	group					1174:1178	the other group	1164:1178	the other group	1164:1178	modesta and the commercial samples derived from these two taxa, while the other group was composed of C. tangshen and its derived commercial samples.
24203345	1	5	theme	commercial	269:278	arg1	samples					280:286	54 commercial samples	266:286	54 commercial samples of Codonopsis Radix available in Chinese, Japanese and Korean markets	266:356	A comparative study of 56 specimens of three medicinally-used Codonopsis taxa collected from China and 54 commercial samples of Codonopsis Radix available in Chinese, Japanese and Korean markets was carried out by quantitative analysis of seven major components: codonopyrrolidium B (1), codonopyrrolidium A (2), tangshenoside I (3), cordifolioidyne B (4), lobetyolinin (5), lobetyolin (6) and lobetyol (7).
24203345	9	6	theme	C.	1473:1474	arg1	tangshen					1476:1483	C. tangshen	1473:1483	C. tangshen from C. pilosula and C. pilosula var	1473:1520	Therefore, 3, 2 and 1 could be chemical markers to differentiate C. tangshen from C. pilosula and C. pilosula var.
24203345	7	7	theme	pilosula	1297:1304	arg1	roots					1285:1289	the roots	1281:1289	the roots of C. pilosula and C. pilosula var	1281:1324	Compound 1 was the main component in the roots of C. pilosula and C. pilosula var.
24203345	2	8	theme	HPLC-DAD	625:632	arg1	method					634:639	a well-established HPLC-DAD method	606:639	a well-established HPLC-DAD method	606:639	The quantitative results, based on a well-established HPLC-DAD method, indicated that the contents of these seven compounds varied considerably among the samples, not only inter-species but also intra-species.
24203345	1	9	theme	samples	280:286	arg1	study					177:181	A comparative study	163:181	A comparative study of 56 specimens of three medicinally-used Codonopsis taxa collected from China and 54 commercial samples of Codonopsis Radix available in Chinese, Japanese and Korean markets	163:356	A comparative study of 56 specimens of three medicinally-used Codonopsis taxa collected from China and 54 commercial samples of Codonopsis Radix available in Chinese, Japanese and Korean markets was carried out by quantitative analysis of seven major components: codonopyrrolidium B (1), codonopyrrolidium A (2), tangshenoside I (3), cordifolioidyne B (4), lobetyolinin (5), lobetyolin (6) and lobetyol (7).
24203345	7	10	theme	var	1322:1324	arg1	roots					1285:1289	the roots	1281:1289	the roots of C. pilosula and C. pilosula var	1281:1324	Compound 1 was the main component in the roots of C. pilosula and C. pilosula var.
24203345	3	11	dep	var	809:811	arg1	pilosula					784:791	pilosula	784:791	pilosula	784:791	C. pilosula and C. pilosula var.
24203345	3	11	dep	var	809:811	arg1	pilosula					800:807	pilosula	800:807	pilosula	800:807	C. pilosula and C. pilosula var.
24203345	4	12	theme	chemical	918:925	arg1	composition					927:937	chemical composition	918:937	chemical composition	918:937	modesta showed similar chemical compositions, while C. tangshen differed considerably from these two in chemical composition.
24203345	0	13	theme	alkaloids	116:124	arg1	contents					92:99	the contents	88:99	the contents of pyrrolidine alkaloids, phenylpropanoid and polyacetylenes	88:160	Quality evaluation of medicinally-used Codonopsis species and Codonopsis Radix based on the contents of pyrrolidine alkaloids, phenylpropanoid and polyacetylenes.
24203345	5	14	theme	main	1009:1012	arg1	groups					1014:1019	two main groups	1005:1019	two main groups	1005:1019	The results of principal component analysis (PCA) indicated that two main groups were classified; one group mainly included C. pilosula, C. pilosula var.
24203345	1	15	from	available	308:316	arg1	markets					350:356	Chinese, Japanese and Korean markets	321:356	Chinese, Japanese and Korean markets	321:356	A comparative study of 56 specimens of three medicinally-used Codonopsis taxa collected from China and 54 commercial samples of Codonopsis Radix available in Chinese, Japanese and Korean markets was carried out by quantitative analysis of seven major components: codonopyrrolidium B (1), codonopyrrolidium A (2), tangshenoside I (3), cordifolioidyne B (4), lobetyolinin (5), lobetyolin (6) and lobetyol (7).
24203345	1	16	theme	codonopyrrolidium	451:467	arg1	components					414:423	seven major components	402:423	seven major components: codonopyrrolidium B (1)	402:448	A comparative study of 56 specimens of three medicinally-used Codonopsis taxa collected from China and 54 commercial samples of Codonopsis Radix available in Chinese, Japanese and Korean markets was carried out by quantitative analysis of seven major components: codonopyrrolidium B (1), codonopyrrolidium A (2), tangshenoside I (3), cordifolioidyne B (4), lobetyolinin (5), lobetyolin (6) and lobetyol (7).
24203345	1	16	theme	codonopyrrolidium	451:467	arg1	2					472:472	2	472:472	2	472:472	A comparative study of 56 specimens of three medicinally-used Codonopsis taxa collected from China and 54 commercial samples of Codonopsis Radix available in Chinese, Japanese and Korean markets was carried out by quantitative analysis of seven major components: codonopyrrolidium B (1), codonopyrrolidium A (2), tangshenoside I (3), cordifolioidyne B (4), lobetyolinin (5), lobetyolin (6) and lobetyol (7).
24203345	1	16	theme	codonopyrrolidium	451:467	arg1	A					469:469	codonopyrrolidium A	451:469	codonopyrrolidium A (2)	451:473	A comparative study of 56 specimens of three medicinally-used Codonopsis taxa collected from China and 54 commercial samples of Codonopsis Radix available in Chinese, Japanese and Korean markets was carried out by quantitative analysis of seven major components: codonopyrrolidium B (1), codonopyrrolidium A (2), tangshenoside I (3), cordifolioidyne B (4), lobetyolinin (5), lobetyolin (6) and lobetyol (7).
24203345	0	17	theme	pyrrolidine	104:114	arg1	polyacetylenes					147:160	polyacetylenes	147:160	polyacetylenes	147:160	Quality evaluation of medicinally-used Codonopsis species and Codonopsis Radix based on the contents of pyrrolidine alkaloids, phenylpropanoid and polyacetylenes.
24203345	0	17	theme	pyrrolidine	104:114	arg1	phenylpropanoid					127:141	phenylpropanoid	127:141	phenylpropanoid	127:141	Quality evaluation of medicinally-used Codonopsis species and Codonopsis Radix based on the contents of pyrrolidine alkaloids, phenylpropanoid and polyacetylenes.
24203345	0	17	theme	pyrrolidine	104:114	arg1	alkaloids					116:124	pyrrolidine alkaloids	104:124	pyrrolidine alkaloids	104:124	Quality evaluation of medicinally-used Codonopsis species and Codonopsis Radix based on the contents of pyrrolidine alkaloids, phenylpropanoid and polyacetylenes.
24203345	1	18	theme	quantitative	377:388	arg1	analysis					390:397	quantitative analysis	377:397	quantitative analysis of seven major components: codonopyrrolidium B (1), codonopyrrolidium A (2), tangshenoside I (3), cordifolioidyne B (4), lobetyolinin (5), lobetyolin (6) and lobetyol (7)	377:568	A comparative study of 56 specimens of three medicinally-used Codonopsis taxa collected from China and 54 commercial samples of Codonopsis Radix available in Chinese, Japanese and Korean markets was carried out by quantitative analysis of seven major components: codonopyrrolidium B (1), codonopyrrolidium A (2), tangshenoside I (3), cordifolioidyne B (4), lobetyolinin (5), lobetyolin (6) and lobetyol (7).
24203345	6	19	theme	commercial	1224:1233	arg1	samples					1235:1241	its derived commercial samples	1212:1241	its derived commercial samples	1212:1241	modesta and the commercial samples derived from these two taxa, while the other group was composed of C. tangshen and its derived commercial samples.
24203345	2	20	dep	indicated	642:650	arg1	only					738:741	only	738:741	only	738:741	The quantitative results, based on a well-established HPLC-DAD method, indicated that the contents of these seven compounds varied considerably among the samples, not only inter-species but also intra-species.
24203345	9	21	from	pilosula	1493:1500	arg1	tangshen					1476:1483	C. tangshen	1473:1483	C. tangshen from C. pilosula and C. pilosula var	1473:1520	Therefore, 3, 2 and 1 could be chemical markers to differentiate C. tangshen from C. pilosula and C. pilosula var.
24203345	8	22	theme	high	1365:1368	arg1	contents					1370:1377	relatively high contents	1354:1377	relatively high contents	1354:1377	modesta, while 3 and 2 had relatively high contents in the roots of C. tangshen.
24203345	1	23	theme	specimens	189:197	arg1	study					177:181	A comparative study	163:181	A comparative study of 56 specimens of three medicinally-used Codonopsis taxa collected from China and 54 commercial samples of Codonopsis Radix available in Chinese, Japanese and Korean markets	163:356	A comparative study of 56 specimens of three medicinally-used Codonopsis taxa collected from China and 54 commercial samples of Codonopsis Radix available in Chinese, Japanese and Korean markets was carried out by quantitative analysis of seven major components: codonopyrrolidium B (1), codonopyrrolidium A (2), tangshenoside I (3), cordifolioidyne B (4), lobetyolinin (5), lobetyolin (6) and lobetyol (7).
24203345	0	24	theme	Quality	0:6	arg1	evaluation					8:17	Quality evaluation	0:17	Quality evaluation of medicinally-used Codonopsis species and Codonopsis Radix	0:77	Quality evaluation of medicinally-used Codonopsis species and Codonopsis Radix based on the contents of pyrrolidine alkaloids, phenylpropanoid and polyacetylenes.
24203345	1	25	theme	Codonopsis	291:300	arg1	Radix					302:306	Codonopsis Radix	291:306	Codonopsis Radix available in Chinese, Japanese and Korean markets	291:356	A comparative study of 56 specimens of three medicinally-used Codonopsis taxa collected from China and 54 commercial samples of Codonopsis Radix available in Chinese, Japanese and Korean markets was carried out by quantitative analysis of seven major components: codonopyrrolidium B (1), codonopyrrolidium A (2), tangshenoside I (3), cordifolioidyne B (4), lobetyolinin (5), lobetyolin (6) and lobetyol (7).
24203345	2	26	theme	quantitative	575:586	arg1	results					588:594	The quantitative results	571:594	The quantitative results	571:594	The quantitative results, based on a well-established HPLC-DAD method, indicated that the contents of these seven compounds varied considerably among the samples, not only inter-species but also intra-species.
24203345	8	27	contain	had	1350:1352	arg2	contents					1370:1377	relatively high contents	1354:1377	relatively high contents	1354:1377	modesta, while 3 and 2 had relatively high contents in the roots of C. tangshen.
24203345	8	27	contain	had	1350:1352	arg1	2					1348:1348	2	1348:1348	2	1348:1348	modesta, while 3 and 2 had relatively high contents in the roots of C. tangshen.
24203345	8	27	contain	had	1350:1352	arg1	3					1342:1342	3	1342:1342	3	1342:1342	modesta, while 3 and 2 had relatively high contents in the roots of C. tangshen.
24203345	0	28	theme	medicinally-used	22:37	arg1	species					50:56	medicinally-used Codonopsis species	22:56	medicinally-used Codonopsis species	22:56	Quality evaluation of medicinally-used Codonopsis species and Codonopsis Radix based on the contents of pyrrolidine alkaloids, phenylpropanoid and polyacetylenes.
24203345	1	29	theme	Radix	302:306	arg1	specimens					189:197	56 specimens	186:197	56 specimens of three medicinally-used Codonopsis taxa collected from China	186:260	A comparative study of 56 specimens of three medicinally-used Codonopsis taxa collected from China and 54 commercial samples of Codonopsis Radix available in Chinese, Japanese and Korean markets was carried out by quantitative analysis of seven major components: codonopyrrolidium B (1), codonopyrrolidium A (2), tangshenoside I (3), cordifolioidyne B (4), lobetyolinin (5), lobetyolin (6) and lobetyol (7).
24203345	1	29	theme	Radix	302:306	arg1	samples					280:286	54 commercial samples	266:286	54 commercial samples of Codonopsis Radix available in Chinese, Japanese and Korean markets	266:356	A comparative study of 56 specimens of three medicinally-used Codonopsis taxa collected from China and 54 commercial samples of Codonopsis Radix available in Chinese, Japanese and Korean markets was carried out by quantitative analysis of seven major components: codonopyrrolidium B (1), codonopyrrolidium A (2), tangshenoside I (3), cordifolioidyne B (4), lobetyolinin (5), lobetyolin (6) and lobetyol (7).
24203345	4	30	theme	similar	829:835	arg1	compositions					846:857	similar chemical compositions	829:857	similar chemical compositions	829:857	modesta showed similar chemical compositions, while C. tangshen differed considerably from these two in chemical composition.
24203345	6	31	theme	commercial	1110:1119	arg1	samples					1121:1127	the commercial samples	1106:1127	the commercial samples derived from these two taxa, while the other group	1106:1178	modesta and the commercial samples derived from these two taxa, while the other group was composed of C. tangshen and its derived commercial samples.
24203345	2	32	theme	compounds	685:693	arg1	contents					661:668	the contents	657:668	the contents of these seven compounds	657:693	The quantitative results, based on a well-established HPLC-DAD method, indicated that the contents of these seven compounds varied considerably among the samples, not only inter-species but also intra-species.
24203345	1	33	theme	available	308:316	arg1	Radix					302:306	Codonopsis Radix	291:306	Codonopsis Radix available in Chinese, Japanese and Korean markets	291:356	A comparative study of 56 specimens of three medicinally-used Codonopsis taxa collected from China and 54 commercial samples of Codonopsis Radix available in Chinese, Japanese and Korean markets was carried out by quantitative analysis of seven major components: codonopyrrolidium B (1), codonopyrrolidium A (2), tangshenoside I (3), cordifolioidyne B (4), lobetyolinin (5), lobetyolin (6) and lobetyol (7).
24203345	1	34	theme	medicinally-used	208:223	arg1	taxa					236:239	three medicinally-used Codonopsis taxa	202:239	three medicinally-used Codonopsis taxa collected from China	202:260	A comparative study of 56 specimens of three medicinally-used Codonopsis taxa collected from China and 54 commercial samples of Codonopsis Radix available in Chinese, Japanese and Korean markets was carried out by quantitative analysis of seven major components: codonopyrrolidium B (1), codonopyrrolidium A (2), tangshenoside I (3), cordifolioidyne B (4), lobetyolinin (5), lobetyolin (6) and lobetyol (7).
24203345	0	35	theme	species	50:56	arg1	evaluation					8:17	Quality evaluation	0:17	Quality evaluation of medicinally-used Codonopsis species and Codonopsis Radix	0:77	Quality evaluation of medicinally-used Codonopsis species and Codonopsis Radix based on the contents of pyrrolidine alkaloids, phenylpropanoid and polyacetylenes.
24203345	8	36	dep	modesta	1327:1333	arg1	while					1336:1340	while	1336:1340	while	1336:1340	modesta, while 3 and 2 had relatively high contents in the roots of C. tangshen.
24203345	1	37	theme	Codonopsis	225:234	arg1	taxa					236:239	three medicinally-used Codonopsis taxa	202:239	three medicinally-used Codonopsis taxa collected from China	202:260	A comparative study of 56 specimens of three medicinally-used Codonopsis taxa collected from China and 54 commercial samples of Codonopsis Radix available in Chinese, Japanese and Korean markets was carried out by quantitative analysis of seven major components: codonopyrrolidium B (1), codonopyrrolidium A (2), tangshenoside I (3), cordifolioidyne B (4), lobetyolinin (5), lobetyolin (6) and lobetyol (7).
24203345	0	38	theme	Codonopsis	39:48	arg1	species					50:56	medicinally-used Codonopsis species	22:56	medicinally-used Codonopsis species	22:56	Quality evaluation of medicinally-used Codonopsis species and Codonopsis Radix based on the contents of pyrrolidine alkaloids, phenylpropanoid and polyacetylenes.
24203345	1	39	theme	Chinese	321:327	arg1	markets					350:356	Chinese, Japanese and Korean markets	321:356	Chinese, Japanese and Korean markets	321:356	A comparative study of 56 specimens of three medicinally-used Codonopsis taxa collected from China and 54 commercial samples of Codonopsis Radix available in Chinese, Japanese and Korean markets was carried out by quantitative analysis of seven major components: codonopyrrolidium B (1), codonopyrrolidium A (2), tangshenoside I (3), cordifolioidyne B (4), lobetyolinin (5), lobetyolin (6) and lobetyol (7).
24203345	4	40	theme	chemical	837:844	arg1	compositions					846:857	similar chemical compositions	829:857	similar chemical compositions	829:857	modesta showed similar chemical compositions, while C. tangshen differed considerably from these two in chemical composition.
24203345	1	41	theme	major	408:412	arg1	lobetyolin					538:547	lobetyolin (6)	538:551	lobetyolin (6)	538:551	A comparative study of 56 specimens of three medicinally-used Codonopsis taxa collected from China and 54 commercial samples of Codonopsis Radix available in Chinese, Japanese and Korean markets was carried out by quantitative analysis of seven major components: codonopyrrolidium B (1), codonopyrrolidium A (2), tangshenoside I (3), cordifolioidyne B (4), lobetyolinin (5), lobetyolin (6) and lobetyol (7).
24203345	1	41	theme	major	408:412	arg1	components					414:423	seven major components	402:423	seven major components: codonopyrrolidium B (1)	402:448	A comparative study of 56 specimens of three medicinally-used Codonopsis taxa collected from China and 54 commercial samples of Codonopsis Radix available in Chinese, Japanese and Korean markets was carried out by quantitative analysis of seven major components: codonopyrrolidium B (1), codonopyrrolidium A (2), tangshenoside I (3), cordifolioidyne B (4), lobetyolinin (5), lobetyolin (6) and lobetyol (7).
24203345	1	41	theme	major	408:412	arg1	lobetyolinin					520:531	lobetyolinin (5)	520:535	lobetyolinin (5)	520:535	A comparative study of 56 specimens of three medicinally-used Codonopsis taxa collected from China and 54 commercial samples of Codonopsis Radix available in Chinese, Japanese and Korean markets was carried out by quantitative analysis of seven major components: codonopyrrolidium B (1), codonopyrrolidium A (2), tangshenoside I (3), cordifolioidyne B (4), lobetyolinin (5), lobetyolin (6) and lobetyol (7).
24203345	1	41	theme	major	408:412	arg1	I					490:490	tangshenoside I	476:490	tangshenoside I (3)	476:494	A comparative study of 56 specimens of three medicinally-used Codonopsis taxa collected from China and 54 commercial samples of Codonopsis Radix available in Chinese, Japanese and Korean markets was carried out by quantitative analysis of seven major components: codonopyrrolidium B (1), codonopyrrolidium A (2), tangshenoside I (3), cordifolioidyne B (4), lobetyolinin (5), lobetyolin (6) and lobetyol (7).
24203345	1	41	theme	major	408:412	arg1	B					513:513	cordifolioidyne B	497:513	cordifolioidyne B (4)	497:517	A comparative study of 56 specimens of three medicinally-used Codonopsis taxa collected from China and 54 commercial samples of Codonopsis Radix available in Chinese, Japanese and Korean markets was carried out by quantitative analysis of seven major components: codonopyrrolidium B (1), codonopyrrolidium A (2), tangshenoside I (3), cordifolioidyne B (4), lobetyolinin (5), lobetyolin (6) and lobetyol (7).
24203345	1	41	theme	major	408:412	arg1	A					469:469	codonopyrrolidium A	451:469	codonopyrrolidium A (2)	451:473	A comparative study of 56 specimens of three medicinally-used Codonopsis taxa collected from China and 54 commercial samples of Codonopsis Radix available in Chinese, Japanese and Korean markets was carried out by quantitative analysis of seven major components: codonopyrrolidium B (1), codonopyrrolidium A (2), tangshenoside I (3), cordifolioidyne B (4), lobetyolinin (5), lobetyolin (6) and lobetyol (7).
24203345	1	41	theme	major	408:412	arg1	lobetyol					557:564	lobetyol (7)	557:568	lobetyol (7)	557:568	A comparative study of 56 specimens of three medicinally-used Codonopsis taxa collected from China and 54 commercial samples of Codonopsis Radix available in Chinese, Japanese and Korean markets was carried out by quantitative analysis of seven major components: codonopyrrolidium B (1), codonopyrrolidium A (2), tangshenoside I (3), cordifolioidyne B (4), lobetyolinin (5), lobetyolin (6) and lobetyol (7).
24203345	7	42	theme	main	1263:1266	arg1	component					1268:1276	the main component	1259:1276	the main component in the roots of C. pilosula and C. pilosula var	1259:1324	Compound 1 was the main component in the roots of C. pilosula and C. pilosula var.
24203345	7	42	theme	main	1263:1266	arg1	Compound					1244:1251	Compound 1	1244:1253	Compound 1	1244:1253	Compound 1 was the main component in the roots of C. pilosula and C. pilosula var.
24203345	2	43	theme	well-established	608:623	arg1	method					634:639	a well-established HPLC-DAD method	606:639	a well-established HPLC-DAD method	606:639	The quantitative results, based on a well-established HPLC-DAD method, indicated that the contents of these seven compounds varied considerably among the samples, not only inter-species but also intra-species.
24203345	1	44	theme	taxa	236:239	arg1	specimens					189:197	56 specimens	186:197	56 specimens of three medicinally-used Codonopsis taxa collected from China	186:260	A comparative study of 56 specimens of three medicinally-used Codonopsis taxa collected from China and 54 commercial samples of Codonopsis Radix available in Chinese, Japanese and Korean markets was carried out by quantitative analysis of seven major components: codonopyrrolidium B (1), codonopyrrolidium A (2), tangshenoside I (3), cordifolioidyne B (4), lobetyolinin (5), lobetyolin (6) and lobetyol (7).
24203345	1	44	theme	taxa	236:239	arg1	samples					280:286	54 commercial samples	266:286	54 commercial samples of Codonopsis Radix available in Chinese, Japanese and Korean markets	266:356	A comparative study of 56 specimens of three medicinally-used Codonopsis taxa collected from China and 54 commercial samples of Codonopsis Radix available in Chinese, Japanese and Korean markets was carried out by quantitative analysis of seven major components: codonopyrrolidium B (1), codonopyrrolidium A (2), tangshenoside I (3), cordifolioidyne B (4), lobetyolinin (5), lobetyolin (6) and lobetyol (7).
24203345	6	45	attach	derived	1129:1135	arg1	taxa					1152:1155	these two taxa	1142:1155	these two taxa	1142:1155	modesta and the commercial samples derived from these two taxa, while the other group was composed of C. tangshen and its derived commercial samples.
24203345	6	45	attach	derived	1129:1135	arg2	samples					1121:1127	the commercial samples	1106:1127	the commercial samples derived from these two taxa, while the other group	1106:1178	modesta and the commercial samples derived from these two taxa, while the other group was composed of C. tangshen and its derived commercial samples.
24203345	1	46	theme	components	414:423	arg1	analysis					390:397	quantitative analysis	377:397	quantitative analysis of seven major components: codonopyrrolidium B (1), codonopyrrolidium A (2), tangshenoside I (3), cordifolioidyne B (4), lobetyolinin (5), lobetyolin (6) and lobetyol (7)	377:568	A comparative study of 56 specimens of three medicinally-used Codonopsis taxa collected from China and 54 commercial samples of Codonopsis Radix available in Chinese, Japanese and Korean markets was carried out by quantitative analysis of seven major components: codonopyrrolidium B (1), codonopyrrolidium A (2), tangshenoside I (3), cordifolioidyne B (4), lobetyolinin (5), lobetyolin (6) and lobetyol (7).
24203345	1	47	theme	tangshenoside	476:488	arg1	I					490:490	tangshenoside I	476:490	tangshenoside I (3)	476:494	A comparative study of 56 specimens of three medicinally-used Codonopsis taxa collected from China and 54 commercial samples of Codonopsis Radix available in Chinese, Japanese and Korean markets was carried out by quantitative analysis of seven major components: codonopyrrolidium B (1), codonopyrrolidium A (2), tangshenoside I (3), cordifolioidyne B (4), lobetyolinin (5), lobetyolin (6) and lobetyol (7).
24203345	1	47	theme	tangshenoside	476:488	arg1	components					414:423	seven major components	402:423	seven major components: codonopyrrolidium B (1)	402:448	A comparative study of 56 specimens of three medicinally-used Codonopsis taxa collected from China and 54 commercial samples of Codonopsis Radix available in Chinese, Japanese and Korean markets was carried out by quantitative analysis of seven major components: codonopyrrolidium B (1), codonopyrrolidium A (2), tangshenoside I (3), cordifolioidyne B (4), lobetyolinin (5), lobetyolin (6) and lobetyol (7).
24203345	1	47	theme	tangshenoside	476:488	arg1	3					493:493	3	493:493	3	493:493	A comparative study of 56 specimens of three medicinally-used Codonopsis taxa collected from China and 54 commercial samples of Codonopsis Radix available in Chinese, Japanese and Korean markets was carried out by quantitative analysis of seven major components: codonopyrrolidium B (1), codonopyrrolidium A (2), tangshenoside I (3), cordifolioidyne B (4), lobetyolinin (5), lobetyolin (6) and lobetyol (7).
24203345	1	48	from	markets	350:356	arg1	available					308:316	available	308:316	available	308:316	A comparative study of 56 specimens of three medicinally-used Codonopsis taxa collected from China and 54 commercial samples of Codonopsis Radix available in Chinese, Japanese and Korean markets was carried out by quantitative analysis of seven major components: codonopyrrolidium B (1), codonopyrrolidium A (2), tangshenoside I (3), cordifolioidyne B (4), lobetyolinin (5), lobetyolin (6) and lobetyol (7).
24203345	1	49	theme	Japanese	330:337	arg1	markets					350:356	Chinese, Japanese and Korean markets	321:356	Chinese, Japanese and Korean markets	321:356	A comparative study of 56 specimens of three medicinally-used Codonopsis taxa collected from China and 54 commercial samples of Codonopsis Radix available in Chinese, Japanese and Korean markets was carried out by quantitative analysis of seven major components: codonopyrrolidium B (1), codonopyrrolidium A (2), tangshenoside I (3), cordifolioidyne B (4), lobetyolinin (5), lobetyolin (6) and lobetyol (7).
24203345	6	50	theme	derived	1216:1222	arg1	samples					1235:1241	its derived commercial samples	1212:1241	its derived commercial samples	1212:1241	modesta and the commercial samples derived from these two taxa, while the other group was composed of C. tangshen and its derived commercial samples.
24203345	5	51	theme	principal	955:963	arg1	analysis					975:982	principal component analysis	955:982	principal component analysis (PCA)	955:988	The results of principal component analysis (PCA) indicated that two main groups were classified; one group mainly included C. pilosula, C. pilosula var.
24203345	5	51	theme	principal	955:963	arg1	PCA					985:987	PCA	985:987	PCA	985:987	The results of principal component analysis (PCA) indicated that two main groups were classified; one group mainly included C. pilosula, C. pilosula var.
24203345	5	52	theme	component	965:973	arg1	analysis					975:982	principal component analysis	955:982	principal component analysis (PCA)	955:988	The results of principal component analysis (PCA) indicated that two main groups were classified; one group mainly included C. pilosula, C. pilosula var.
24203345	5	52	theme	component	965:973	arg1	PCA					985:987	PCA	985:987	PCA	985:987	The results of principal component analysis (PCA) indicated that two main groups were classified; one group mainly included C. pilosula, C. pilosula var.
24203345	7	53	from	component	1268:1276	arg1	roots					1285:1289	the roots	1281:1289	the roots of C. pilosula and C. pilosula var	1281:1324	Compound 1 was the main component in the roots of C. pilosula and C. pilosula var.
24203345	1	54	theme	codonopyrrolidium	426:442	arg1	1					447:447	1	447:447	1	447:447	A comparative study of 56 specimens of three medicinally-used Codonopsis taxa collected from China and 54 commercial samples of Codonopsis Radix available in Chinese, Japanese and Korean markets was carried out by quantitative analysis of seven major components: codonopyrrolidium B (1), codonopyrrolidium A (2), tangshenoside I (3), cordifolioidyne B (4), lobetyolinin (5), lobetyolin (6) and lobetyol (7).
24203345	1	54	theme	codonopyrrolidium	426:442	arg1	B					444:444	codonopyrrolidium B	426:444	seven major components: codonopyrrolidium B (1)	402:448	A comparative study of 56 specimens of three medicinally-used Codonopsis taxa collected from China and 54 commercial samples of Codonopsis Radix available in Chinese, Japanese and Korean markets was carried out by quantitative analysis of seven major components: codonopyrrolidium B (1), codonopyrrolidium A (2), tangshenoside I (3), cordifolioidyne B (4), lobetyolinin (5), lobetyolin (6) and lobetyol (7).
24203345	5	55	theme	analysis	975:982	arg1	results					944:950	The results	940:950	The results of principal component analysis (PCA)	940:988	The results of principal component analysis (PCA) indicated that two main groups were classified; one group mainly included C. pilosula, C. pilosula var.
24203345	6	56	link	derived	1216:1222	arg1	samples					1235:1241	its derived commercial samples	1212:1241	its derived commercial samples	1212:1241	modesta and the commercial samples derived from these two taxa, while the other group was composed of C. tangshen and its derived commercial samples.
24203345	1	57	dep	components	414:423	arg1	1					447:447	1	447:447	1	447:447	A comparative study of 56 specimens of three medicinally-used Codonopsis taxa collected from China and 54 commercial samples of Codonopsis Radix available in Chinese, Japanese and Korean markets was carried out by quantitative analysis of seven major components: codonopyrrolidium B (1), codonopyrrolidium A (2), tangshenoside I (3), cordifolioidyne B (4), lobetyolinin (5), lobetyolin (6) and lobetyol (7).
24203345	1	57	dep	components	414:423	arg1	B					444:444	codonopyrrolidium B	426:444	seven major components: codonopyrrolidium B (1)	402:448	A comparative study of 56 specimens of three medicinally-used Codonopsis taxa collected from China and 54 commercial samples of Codonopsis Radix available in Chinese, Japanese and Korean markets was carried out by quantitative analysis of seven major components: codonopyrrolidium B (1), codonopyrrolidium A (2), tangshenoside I (3), cordifolioidyne B (4), lobetyolinin (5), lobetyolin (6) and lobetyol (7).
24203345	9	58	theme	chemical	1439:1446	arg1	markers					1448:1454	chemical markers	1439:1454	chemical markers	1439:1454	Therefore, 3, 2 and 1 could be chemical markers to differentiate C. tangshen from C. pilosula and C. pilosula var.
24203345	0	59	theme	Radix	73:77	arg1	evaluation					8:17	Quality evaluation	0:17	Quality evaluation of medicinally-used Codonopsis species and Codonopsis Radix	0:77	Quality evaluation of medicinally-used Codonopsis species and Codonopsis Radix based on the contents of pyrrolidine alkaloids, phenylpropanoid and polyacetylenes.
24203345	1	60	theme	Korean	343:348	arg1	markets					350:356	Chinese, Japanese and Korean markets	321:356	Chinese, Japanese and Korean markets	321:356	A comparative study of 56 specimens of three medicinally-used Codonopsis taxa collected from China and 54 commercial samples of Codonopsis Radix available in Chinese, Japanese and Korean markets was carried out by quantitative analysis of seven major components: codonopyrrolidium B (1), codonopyrrolidium A (2), tangshenoside I (3), cordifolioidyne B (4), lobetyolinin (5), lobetyolin (6) and lobetyol (7).
24203345	4	61	theme	C.	866:867	arg1	tangshen					869:876	C. tangshen	866:876	C. tangshen	866:876	modesta showed similar chemical compositions, while C. tangshen differed considerably from these two in chemical composition.
27184456	6	0	theme	@	1088:1088	arg1	ChiCol					1089:1094	the HABG@ChiCol and TCPBG@ChiCol composites	1080:1122	ChiCol	1089:1094	Compressive modulus of 459.01 and 435.95 MPa with compressive strength of 22.73 and 17.89 MPa were observed for the HABG@ChiCol and TCPBG@ChiCol composites, respectively.
27184456	0	1	dep	in	21:22	arg1	vitro					24:28	vitro	24:28	vitro	24:28	Physico-chemical and in vitro cellular properties of different calcium phosphate-bioactive glass composite chitosan-collagen (CaP@ChiCol) for bone scaffolds.
27184456	1	2	theme	mixing	407:412	arg1	15Ca:80Si:5P					431:442	15Ca:80Si:5P	431:442	15Ca:80Si:5P	431:442	In the present study, scaffolds for bone tissue engineering applications were made by immersing the inorganic phases of three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL) as a binder in an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol).
27184456	1	2	theme	mixing	407:412	arg1	BG					446:447	BG	446:447	BG	446:447	In the present study, scaffolds for bone tissue engineering applications were made by immersing the inorganic phases of three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL) as a binder in an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol).
27184456	1	2	theme	mixing	407:412	arg1	glass					424:428	three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass	278:428	three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL)	278:476	In the present study, scaffolds for bone tissue engineering applications were made by immersing the inorganic phases of three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL) as a binder in an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol).
27184456	4	3	theme	compressive	768:778	arg1	strength					780:787	the compressive strength	764:787	the compressive strength	764:787	The investigation indicated that the compressive strength was controlled by the types of CaP.
27184456	1	4	theme	@	553:553	arg1	ChiCol					554:559	CaPBG@ChiCol	548:559	CaPBG@ChiCol	548:559	In the present study, scaffolds for bone tissue engineering applications were made by immersing the inorganic phases of three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL) as a binder in an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol).
27184456	1	4	theme	@	553:553	arg1	matrix					540:545	chitosan/collagen (ChiCol) matrix	513:545	chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol)	513:560	In the present study, scaffolds for bone tissue engineering applications were made by immersing the inorganic phases of three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL) as a binder in an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol).
27184456	1	5	theme	glass	424:428	arg1	binder					483:488	a binder	481:488	a binder in an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol)	481:560	In the present study, scaffolds for bone tissue engineering applications were made by immersing the inorganic phases of three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL) as a binder in an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol).
27184456	1	5	theme	glass	424:428	arg1	phases					268:273	the inorganic phases	254:273	the inorganic phases of three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL)	254:476	In the present study, scaffolds for bone tissue engineering applications were made by immersing the inorganic phases of three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL) as a binder in an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol).
27184456	6	6	theme	17.89 MPa	1052:1060	arg1	strength					1030:1037	compressive strength	1018:1037	compressive strength of 22.73 and 17.89 MPa	1018:1060	Compressive modulus of 459.01 and 435.95 MPa with compressive strength of 22.73 and 17.89 MPa were observed for the HABG@ChiCol and TCPBG@ChiCol composites, respectively.
27184456	3	7	theme	mechanical	633:642	arg1	properties					644:653	The mechanical properties	629:653	The mechanical properties	629:653	The mechanical properties and in vitro growth of rat osteoblast-like UMR-106 cells were investigated.
27184456	7	8	theme	In	1139:1140	arg1	tests					1184:1188	In vitro cell availability and proliferation tests	1139:1188	In vitro cell availability and proliferation tests	1139:1188	In vitro cell availability and proliferation tests confirmed the osteoblast attachment and growth on the CaPBG@ChiCol surface.
27184456	1	9	theme	bone	194:197	arg1	applications					218:229	bone tissue engineering applications	194:229	bone tissue engineering applications	194:229	In the present study, scaffolds for bone tissue engineering applications were made by immersing the inorganic phases of three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL) as a binder in an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol).
27184456	1	10	theme	engineering	206:216	arg1	applications					218:229	bone tissue engineering applications	194:229	bone tissue engineering applications	194:229	In the present study, scaffolds for bone tissue engineering applications were made by immersing the inorganic phases of three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL) as a binder in an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol).
27184456	8	11	theme	higher	1343:1348	arg1	density					1355:1361	the higher cell density	1339:1361	the higher cell density	1339:1361	Comparing the scaffolds, cells grown on the BCPBG based composite showed the higher cell density.
27184456	1	12	from	binder	483:488	arg1	phase					504:508	an organic phase	493:508	an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol)	493:560	In the present study, scaffolds for bone tissue engineering applications were made by immersing the inorganic phases of three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL) as a binder in an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol).
27184456	2	13	theme	freeze	597:602	arg1	drying					604:609	freeze drying the combinations	597:626	freeze drying the combinations	597:626	Porous scaffolds were obtained by freeze drying the combinations.
27184456	5	14	theme	BCPBG	944:948	arg1	composite					957:965	the BCPBG@ChiCol composite	940:965	the BCPBG@ChiCol composite	940:965	The highest compressive modulus of the composites was 479.77 MPa (23.84 MPa for compressive strength) which is for the BCPBG@ChiCol composite.
27184456	1	15	theme	biphasic	373:380	arg1	CaPs					313:316	three different calcium phosphate (CaPs)	278:317	three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL)	278:476	In the present study, scaffolds for bone tissue engineering applications were made by immersing the inorganic phases of three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL) as a binder in an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol).
27184456	1	15	theme	biphasic	373:380	arg1	phosphate					390:398	biphasic calcium phosphate	373:398	biphasic calcium phosphate	373:398	In the present study, scaffolds for bone tissue engineering applications were made by immersing the inorganic phases of three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL) as a binder in an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol).
27184456	4	16	theme	CaP	820:822	arg1	types					811:815	the types	807:815	the types of CaP	807:822	The investigation indicated that the compressive strength was controlled by the types of CaP.
27184456	6	17	theme	@	1105:1105	arg1	composites					1113:1122	the HABG@ChiCol and TCPBG@ChiCol composites	1080:1122	composites	1113:1122	Compressive modulus of 459.01 and 435.95 MPa with compressive strength of 22.73 and 17.89 MPa were observed for the HABG@ChiCol and TCPBG@ChiCol composites, respectively.
27184456	7	18	theme	ChiCol	1250:1255	arg1	surface					1257:1263	the CaPBG@ChiCol surface	1240:1263	the CaPBG@ChiCol surface	1240:1263	In vitro cell availability and proliferation tests confirmed the osteoblast attachment and growth on the CaPBG@ChiCol surface.
27184456	3	19	theme	cells	706:710	arg1	growth					668:673	in vitro growth	659:673	in vitro growth	659:673	The mechanical properties and in vitro growth of rat osteoblast-like UMR-106 cells were investigated.
27184456	3	19	theme	cells	706:710	arg1	properties					644:653	The mechanical properties	629:653	The mechanical properties	629:653	The mechanical properties and in vitro growth of rat osteoblast-like UMR-106 cells were investigated.
27184456	5	20	theme	ChiCol	950:955	arg1	composite					957:965	the BCPBG@ChiCol composite	940:965	the BCPBG@ChiCol composite	940:965	The highest compressive modulus of the composites was 479.77 MPa (23.84 MPa for compressive strength) which is for the BCPBG@ChiCol composite.
27184456	9	21	theme	BCPBG	1389:1393	arg1	ChiCol					1395:1400	BCPBG@ChiCol	1389:1400	BCPBG@ChiCol	1389:1400	To test its bioactivity, BCPBG@ChiCol was chosen for MTT and ALP assays on UMR-106 cells.
27184456	9	22	from	assays	1429:1434	arg1	cells					1447:1451	UMR-106 cells	1439:1451	UMR-106 cells	1439:1451	To test its bioactivity, BCPBG@ChiCol was chosen for MTT and ALP assays on UMR-106 cells.
27184456	7	23	theme	CaPBG	1244:1248	arg1	surface					1257:1263	the CaPBG@ChiCol surface	1240:1263	the CaPBG@ChiCol surface	1240:1263	In vitro cell availability and proliferation tests confirmed the osteoblast attachment and growth on the CaPBG@ChiCol surface.
27184456	3	24	theme	osteoblast-like	682:696	arg1	cells					706:710	rat osteoblast-like UMR-106 cells	678:710	rat osteoblast-like UMR-106 cells	678:710	The mechanical properties and in vitro growth of rat osteoblast-like UMR-106 cells were investigated.
27184456	0	25	theme	calcium	63:69	arg1	Physico-chemical					0:15	Physico-chemical	0:15	Physico-chemical	0:15	Physico-chemical and in vitro cellular properties of different calcium phosphate-bioactive glass composite chitosan-collagen (CaP@ChiCol) for bone scaffolds.
27184456	11	26	theme	bone	1640:1643	arg1	regeneration					1645:1656	bone regeneration	1640:1656	bone regeneration	1640:1656	Therefore, ChiCol-fabricated BCPBG scaffold shows promise for bone regeneration.
27184456	0	27	theme	glass	91:95	arg1	ChiCol					130:135	CaP@ChiCol	126:135	CaP@ChiCol	126:135	Physico-chemical and in vitro cellular properties of different calcium phosphate-bioactive glass composite chitosan-collagen (CaP@ChiCol) for bone scaffolds.
27184456	0	27	theme	glass	91:95	arg1	chitosan-collagen					107:123	glass composite chitosan-collagen	91:123	glass composite chitosan-collagen (CaP@ChiCol) for bone scaffolds	91:155	Physico-chemical and in vitro cellular properties of different calcium phosphate-bioactive glass composite chitosan-collagen (CaP@ChiCol) for bone scaffolds.
27184456	1	28	theme	calcium	382:388	arg1	CaPs					313:316	three different calcium phosphate (CaPs)	278:317	three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL)	278:476	In the present study, scaffolds for bone tissue engineering applications were made by immersing the inorganic phases of three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL) as a binder in an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol).
27184456	1	28	theme	calcium	382:388	arg1	phosphate					390:398	biphasic calcium phosphate	373:398	biphasic calcium phosphate	373:398	In the present study, scaffolds for bone tissue engineering applications were made by immersing the inorganic phases of three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL) as a binder in an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol).
27184456	7	29	dep	cell	1148:1151	arg1	availability					1153:1164	availability	1153:1164	availability	1153:1164	In vitro cell availability and proliferation tests confirmed the osteoblast attachment and growth on the CaPBG@ChiCol surface.
27184456	9	30	theme	MTT	1417:1419	arg1	assays					1429:1434	MTT and ALP assays	1417:1434	MTT and ALP assays on UMR-106 cells	1417:1451	To test its bioactivity, BCPBG@ChiCol was chosen for MTT and ALP assays on UMR-106 cells.
27184456	5	31	theme	compressive	837:847	arg1	479.77 MPa					879:888	479.77 MPa	879:888	479.77 MPa (23.84 MPa for compressive strength) which is for the BCPBG@ChiCol composite	879:965	The highest compressive modulus of the composites was 479.77 MPa (23.84 MPa for compressive strength) which is for the BCPBG@ChiCol composite.
27184456	5	31	theme	compressive	837:847	arg1	modulus					849:855	The highest compressive modulus	825:855	The highest compressive modulus of the composites	825:873	The highest compressive modulus of the composites was 479.77 MPa (23.84 MPa for compressive strength) which is for the BCPBG@ChiCol composite.
27184456	0	32	theme	CaP	126:128	arg1	ChiCol					130:135	CaP@ChiCol	126:135	CaP@ChiCol	126:135	Physico-chemical and in vitro cellular properties of different calcium phosphate-bioactive glass composite chitosan-collagen (CaP@ChiCol) for bone scaffolds.
27184456	0	32	theme	CaP	126:128	arg1	chitosan-collagen					107:123	glass composite chitosan-collagen	91:123	glass composite chitosan-collagen (CaP@ChiCol) for bone scaffolds	91:155	Physico-chemical and in vitro cellular properties of different calcium phosphate-bioactive glass composite chitosan-collagen (CaP@ChiCol) for bone scaffolds.
27184456	1	33	theme	tricalcium	341:350	arg1	CaPs					313:316	three different calcium phosphate (CaPs)	278:317	three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL)	278:476	In the present study, scaffolds for bone tissue engineering applications were made by immersing the inorganic phases of three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL) as a binder in an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol).
27184456	1	33	theme	tricalcium	341:350	arg1	TCP					363:365	TCP	363:365	TCP	363:365	In the present study, scaffolds for bone tissue engineering applications were made by immersing the inorganic phases of three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL) as a binder in an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol).
27184456	1	33	theme	tricalcium	341:350	arg1	phosphate					352:360	tricalcium phosphate	341:360	tricalcium phosphate (TCP)	341:366	In the present study, scaffolds for bone tissue engineering applications were made by immersing the inorganic phases of three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL) as a binder in an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol).
27184456	9	34	theme	ALP	1425:1427	arg1	assays					1429:1434	MTT and ALP assays	1417:1434	MTT and ALP assays on UMR-106 cells	1417:1451	To test its bioactivity, BCPBG@ChiCol was chosen for MTT and ALP assays on UMR-106 cells.
27184456	7	35	dep	In	1139:1140	arg1	vitro					1142:1146	vitro	1142:1146	vitro	1142:1146	In vitro cell availability and proliferation tests confirmed the osteoblast attachment and growth on the CaPBG@ChiCol surface.
27184456	1	36	theme	different	284:292	arg1	phosphate					390:398	biphasic calcium phosphate	373:398	biphasic calcium phosphate	373:398	In the present study, scaffolds for bone tissue engineering applications were made by immersing the inorganic phases of three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL) as a binder in an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol).
27184456	1	36	theme	different	284:292	arg1	CaPs					313:316	three different calcium phosphate (CaPs)	278:317	three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL)	278:476	In the present study, scaffolds for bone tissue engineering applications were made by immersing the inorganic phases of three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL) as a binder in an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol).
27184456	1	36	theme	different	284:292	arg1	hydroxyapatite					320:333	hydroxyapatite	320:333	hydroxyapatite (HA)	320:338	In the present study, scaffolds for bone tissue engineering applications were made by immersing the inorganic phases of three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL) as a binder in an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol).
27184456	1	36	theme	different	284:292	arg1	phosphate					352:360	tricalcium phosphate	341:360	tricalcium phosphate (TCP)	341:366	In the present study, scaffolds for bone tissue engineering applications were made by immersing the inorganic phases of three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL) as a binder in an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol).
27184456	5	37	theme	composites	864:873	arg1	479.77 MPa					879:888	479.77 MPa	879:888	479.77 MPa (23.84 MPa for compressive strength) which is for the BCPBG@ChiCol composite	879:965	The highest compressive modulus of the composites was 479.77 MPa (23.84 MPa for compressive strength) which is for the BCPBG@ChiCol composite.
27184456	5	37	theme	composites	864:873	arg1	modulus					849:855	The highest compressive modulus	825:855	The highest compressive modulus of the composites	825:873	The highest compressive modulus of the composites was 479.77 MPa (23.84 MPa for compressive strength) which is for the BCPBG@ChiCol composite.
27184456	2	38	dep	drying	604:609	arg1	combinations					615:626	the combinations	611:626	freeze drying the combinations	597:626	Porous scaffolds were obtained by freeze drying the combinations.
27184456	1	39	theme	organic	496:502	arg1	phase					504:508	an organic phase	493:508	an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol)	493:560	In the present study, scaffolds for bone tissue engineering applications were made by immersing the inorganic phases of three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL) as a binder in an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol).
27184456	10	40	theme	ALP	1526:1528	arg1	activity					1530:1537	higher ALP activity	1519:1537	higher ALP activity	1519:1537	The results indicated that the UMR-106 cells were viable and had higher ALP activity as the culturing times were increased.
27184456	0	41	theme	cellular	30:37	arg1	properties					39:48	cellular properties	30:48	cellular properties	30:48	Physico-chemical and in vitro cellular properties of different calcium phosphate-bioactive glass composite chitosan-collagen (CaP@ChiCol) for bone scaffolds.
27184456	1	42	theme	phosphate	302:310	arg1	phosphate					390:398	biphasic calcium phosphate	373:398	biphasic calcium phosphate	373:398	In the present study, scaffolds for bone tissue engineering applications were made by immersing the inorganic phases of three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL) as a binder in an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol).
27184456	1	42	theme	phosphate	302:310	arg1	CaPs					313:316	three different calcium phosphate (CaPs)	278:317	three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL)	278:476	In the present study, scaffolds for bone tissue engineering applications were made by immersing the inorganic phases of three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL) as a binder in an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol).
27184456	1	42	theme	phosphate	302:310	arg1	hydroxyapatite					320:333	hydroxyapatite	320:333	hydroxyapatite (HA)	320:338	In the present study, scaffolds for bone tissue engineering applications were made by immersing the inorganic phases of three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL) as a binder in an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol).
27184456	1	42	theme	phosphate	302:310	arg1	phosphate					352:360	tricalcium phosphate	341:360	tricalcium phosphate (TCP)	341:366	In the present study, scaffolds for bone tissue engineering applications were made by immersing the inorganic phases of three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL) as a binder in an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol).
27184456	11	43	theme	ChiCol-fabricated	1589:1605	arg1	scaffold					1613:1620	ChiCol-fabricated BCPBG scaffold	1589:1620	ChiCol-fabricated BCPBG scaffold	1589:1620	Therefore, ChiCol-fabricated BCPBG scaffold shows promise for bone regeneration.
27184456	1	44	theme	CaPs	313:316	arg1	15Ca:80Si:5P					431:442	15Ca:80Si:5P	431:442	15Ca:80Si:5P	431:442	In the present study, scaffolds for bone tissue engineering applications were made by immersing the inorganic phases of three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL) as a binder in an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol).
27184456	1	44	theme	CaPs	313:316	arg1	BG					446:447	BG	446:447	BG	446:447	In the present study, scaffolds for bone tissue engineering applications were made by immersing the inorganic phases of three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL) as a binder in an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol).
27184456	1	44	theme	CaPs	313:316	arg1	glass					424:428	three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass	278:428	three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL)	278:476	In the present study, scaffolds for bone tissue engineering applications were made by immersing the inorganic phases of three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL) as a binder in an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol).
27184456	8	45	theme	BCPBG	1310:1314	arg1	composite					1322:1330	the BCPBG based composite	1306:1330	the BCPBG based composite	1306:1330	Comparing the scaffolds, cells grown on the BCPBG based composite showed the higher cell density.
27184456	10	46	theme	culturing	1546:1554	arg1	times					1556:1560	the culturing times	1542:1560	the culturing times	1542:1560	The results indicated that the UMR-106 cells were viable and had higher ALP activity as the culturing times were increased.
27184456	5	47	theme	compressive	905:915	arg1	strength					917:924	compressive strength	905:924	compressive strength	905:924	The highest compressive modulus of the composites was 479.77 MPa (23.84 MPa for compressive strength) which is for the BCPBG@ChiCol composite.
27184456	13	48	dep	Res	1705:1707	arg1	1758-1766					1738:1746	1758-1766	1738:1746	1758-1766	1738:1746	J Biomed Mater Res Part B: Appl Biomater, 105B: 1758-1766, 2017.
27184456	13	48	dep	Res	1705:1707	arg1	105B					1732:1735	105B	1732:1735	105B	1732:1735	J Biomed Mater Res Part B: Appl Biomater, 105B: 1758-1766, 2017.
27184456	13	48	dep	Res	1705:1707	arg1	B					1714:1714	Part B	1709:1714	J Biomed Mater Res Part B: Appl Biomater, 105B: 1758-1766, 2017.	1690:1753	J Biomed Mater Res Part B: Appl Biomater, 105B: 1758-1766, 2017.
27184456	13	48	dep	Res	1705:1707	arg1	Biomater					1722:1729	Biomater	1722:1729	Biomater	1722:1729	J Biomed Mater Res Part B: Appl Biomater, 105B: 1758-1766, 2017.
27184456	6	49	theme	compressive	1018:1028	arg1	strength					1030:1037	compressive strength	1018:1037	compressive strength of 22.73 and 17.89 MPa	1018:1060	Compressive modulus of 459.01 and 435.95 MPa with compressive strength of 22.73 and 17.89 MPa were observed for the HABG@ChiCol and TCPBG@ChiCol composites, respectively.
27184456	7	50	dep	attachment	1215:1224	arg1	the					1200:1202	the	1200:1202	the	1200:1202	In vitro cell availability and proliferation tests confirmed the osteoblast attachment and growth on the CaPBG@ChiCol surface.
27184456	7	51	theme	cell	1148:1151	arg1	tests					1184:1188	In vitro cell availability and proliferation tests	1139:1188	In vitro cell availability and proliferation tests	1139:1188	In vitro cell availability and proliferation tests confirmed the osteoblast attachment and growth on the CaPBG@ChiCol surface.
27184456	6	52	theme	459.01	991:996	arg1	modulus					980:986	Compressive modulus	968:986	Compressive modulus of 459.01 and 435.95 MPa with compressive strength of 22.73 and 17.89 MPa	968:1060	Compressive modulus of 459.01 and 435.95 MPa with compressive strength of 22.73 and 17.89 MPa were observed for the HABG@ChiCol and TCPBG@ChiCol composites, respectively.
27184456	1	53	theme	inorganic	258:266	arg1	binder					483:488	a binder	481:488	a binder in an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol)	481:560	In the present study, scaffolds for bone tissue engineering applications were made by immersing the inorganic phases of three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL) as a binder in an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol).
27184456	1	53	theme	inorganic	258:266	arg1	phases					268:273	the inorganic phases	254:273	the inorganic phases of three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL)	254:476	In the present study, scaffolds for bone tissue engineering applications were made by immersing the inorganic phases of three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL) as a binder in an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol).
27184456	10	54	contain	had	1515:1517	arg2	activity					1530:1537	higher ALP activity	1519:1537	higher ALP activity	1519:1537	The results indicated that the UMR-106 cells were viable and had higher ALP activity as the culturing times were increased.
27184456	10	54	contain	had	1515:1517	arg1	viable					1504:1509	viable	1504:1509	viable	1504:1509	The results indicated that the UMR-106 cells were viable and had higher ALP activity as the culturing times were increased.
27184456	10	54	contain	had	1515:1517	arg1	cells					1493:1497	the UMR-106 cells	1481:1497	the UMR-106 cells	1481:1497	The results indicated that the UMR-106 cells were viable and had higher ALP activity as the culturing times were increased.
27184456	6	55	theme	HABG	1084:1087	arg1	ChiCol					1089:1094	the HABG@ChiCol and TCPBG@ChiCol composites	1080:1122	ChiCol	1089:1094	Compressive modulus of 459.01 and 435.95 MPa with compressive strength of 22.73 and 17.89 MPa were observed for the HABG@ChiCol and TCPBG@ChiCol composites, respectively.
27184456	6	56	theme	22.73	1042:1046	arg1	strength					1030:1037	compressive strength	1018:1037	compressive strength of 22.73 and 17.89 MPa	1018:1060	Compressive modulus of 459.01 and 435.95 MPa with compressive strength of 22.73 and 17.89 MPa were observed for the HABG@ChiCol and TCPBG@ChiCol composites, respectively.
27184456	1	57	theme	present	165:171	arg1	study					173:177	the present study	161:177	the present study	161:177	In the present study, scaffolds for bone tissue engineering applications were made by immersing the inorganic phases of three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL) as a binder in an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol).
27184456	1	58	theme	CaPBG	548:552	arg1	ChiCol					554:559	CaPBG@ChiCol	548:559	CaPBG@ChiCol	548:559	In the present study, scaffolds for bone tissue engineering applications were made by immersing the inorganic phases of three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL) as a binder in an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol).
27184456	1	58	theme	CaPBG	548:552	arg1	matrix					540:545	chitosan/collagen (ChiCol) matrix	513:545	chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol)	513:560	In the present study, scaffolds for bone tissue engineering applications were made by immersing the inorganic phases of three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL) as a binder in an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol).
27184456	1	59	theme	bioactive	414:422	arg1	15Ca:80Si:5P					431:442	15Ca:80Si:5P	431:442	15Ca:80Si:5P	431:442	In the present study, scaffolds for bone tissue engineering applications were made by immersing the inorganic phases of three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL) as a binder in an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol).
27184456	1	59	theme	bioactive	414:422	arg1	BG					446:447	BG	446:447	BG	446:447	In the present study, scaffolds for bone tissue engineering applications were made by immersing the inorganic phases of three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL) as a binder in an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol).
27184456	1	59	theme	bioactive	414:422	arg1	glass					424:428	three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass	278:428	three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL)	278:476	In the present study, scaffolds for bone tissue engineering applications were made by immersing the inorganic phases of three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL) as a binder in an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol).
27184456	3	60	theme	in	659:660	arg1	growth					668:673	in vitro growth	659:673	in vitro growth	659:673	The mechanical properties and in vitro growth of rat osteoblast-like UMR-106 cells were investigated.
27184456	0	61	theme	bone	142:145	arg1	scaffolds					147:155	bone scaffolds	142:155	bone scaffolds	142:155	Physico-chemical and in vitro cellular properties of different calcium phosphate-bioactive glass composite chitosan-collagen (CaP@ChiCol) for bone scaffolds.
27184456	1	62	theme	tissue	199:204	arg1	applications					218:229	bone tissue engineering applications	194:229	bone tissue engineering applications	194:229	In the present study, scaffolds for bone tissue engineering applications were made by immersing the inorganic phases of three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL) as a binder in an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol).
27184456	8	63	theme	cell	1350:1353	arg1	density					1355:1361	the higher cell density	1339:1361	the higher cell density	1339:1361	Comparing the scaffolds, cells grown on the BCPBG based composite showed the higher cell density.
27184456	3	64	theme	rat	678:680	arg1	cells					706:710	rat osteoblast-like UMR-106 cells	678:710	rat osteoblast-like UMR-106 cells	678:710	The mechanical properties and in vitro growth of rat osteoblast-like UMR-106 cells were investigated.
27184456	6	65	theme	ChiCol	1106:1111	arg1	composites					1113:1122	the HABG@ChiCol and TCPBG@ChiCol composites	1080:1122	composites	1113:1122	Compressive modulus of 459.01 and 435.95 MPa with compressive strength of 22.73 and 17.89 MPa were observed for the HABG@ChiCol and TCPBG@ChiCol composites, respectively.
27184456	5	66	theme	@	949:949	arg1	composite					957:965	the BCPBG@ChiCol composite	940:965	the BCPBG@ChiCol composite	940:965	The highest compressive modulus of the composites was 479.77 MPa (23.84 MPa for compressive strength) which is for the BCPBG@ChiCol composite.
27184456	5	67	theme	highest	829:835	arg1	479.77 MPa					879:888	479.77 MPa	879:888	479.77 MPa (23.84 MPa for compressive strength) which is for the BCPBG@ChiCol composite	879:965	The highest compressive modulus of the composites was 479.77 MPa (23.84 MPa for compressive strength) which is for the BCPBG@ChiCol composite.
27184456	5	67	theme	highest	829:835	arg1	modulus					849:855	The highest compressive modulus	825:855	The highest compressive modulus of the composites	825:873	The highest compressive modulus of the composites was 479.77 MPa (23.84 MPa for compressive strength) which is for the BCPBG@ChiCol composite.
27184456	6	68	theme	TCPBG	1100:1104	arg1	composites					1113:1122	the HABG@ChiCol and TCPBG@ChiCol composites	1080:1122	composites	1113:1122	Compressive modulus of 459.01 and 435.95 MPa with compressive strength of 22.73 and 17.89 MPa were observed for the HABG@ChiCol and TCPBG@ChiCol composites, respectively.
27184456	5	69	dep	479.77 MPa	879:888	arg1	23.84 MPa					891:899	23.84 MPa	891:899	23.84 MPa for compressive strength	891:924	The highest compressive modulus of the composites was 479.77 MPa (23.84 MPa for compressive strength) which is for the BCPBG@ChiCol composite.
27184456	1	70	theme	chitosan/collagen	513:529	arg1	ChiCol					554:559	CaPBG@ChiCol	548:559	CaPBG@ChiCol	548:559	In the present study, scaffolds for bone tissue engineering applications were made by immersing the inorganic phases of three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL) as a binder in an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol).
27184456	1	70	theme	chitosan/collagen	513:529	arg1	matrix					540:545	chitosan/collagen (ChiCol) matrix	513:545	chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol)	513:560	In the present study, scaffolds for bone tissue engineering applications were made by immersing the inorganic phases of three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL) as a binder in an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol).
27184456	11	71	theme	BCPBG	1607:1611	arg1	scaffold					1613:1620	ChiCol-fabricated BCPBG scaffold	1589:1620	ChiCol-fabricated BCPBG scaffold	1589:1620	Therefore, ChiCol-fabricated BCPBG scaffold shows promise for bone regeneration.
27184456	9	72	theme	@	1394:1394	arg1	ChiCol					1395:1400	BCPBG@ChiCol	1389:1400	BCPBG@ChiCol	1389:1400	To test its bioactivity, BCPBG@ChiCol was chosen for MTT and ALP assays on UMR-106 cells.
27184456	7	73	theme	@	1249:1249	arg1	surface					1257:1263	the CaPBG@ChiCol surface	1240:1263	the CaPBG@ChiCol surface	1240:1263	In vitro cell availability and proliferation tests confirmed the osteoblast attachment and growth on the CaPBG@ChiCol surface.
27184456	1	74	theme	ChiCol	532:537	arg1	ChiCol					554:559	CaPBG@ChiCol	548:559	CaPBG@ChiCol	548:559	In the present study, scaffolds for bone tissue engineering applications were made by immersing the inorganic phases of three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL) as a binder in an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol).
27184456	1	74	theme	ChiCol	532:537	arg1	matrix					540:545	chitosan/collagen (ChiCol) matrix	513:545	chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol)	513:560	In the present study, scaffolds for bone tissue engineering applications were made by immersing the inorganic phases of three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL) as a binder in an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol).
27184456	3	75	theme	UMR-106	698:704	arg1	cells					706:710	rat osteoblast-like UMR-106 cells	678:710	rat osteoblast-like UMR-106 cells	678:710	The mechanical properties and in vitro growth of rat osteoblast-like UMR-106 cells were investigated.
27184456	0	76	theme	composite	97:105	arg1	ChiCol					130:135	CaP@ChiCol	126:135	CaP@ChiCol	126:135	Physico-chemical and in vitro cellular properties of different calcium phosphate-bioactive glass composite chitosan-collagen (CaP@ChiCol) for bone scaffolds.
27184456	0	76	theme	composite	97:105	arg1	chitosan-collagen					107:123	glass composite chitosan-collagen	91:123	glass composite chitosan-collagen (CaP@ChiCol) for bone scaffolds	91:155	Physico-chemical and in vitro cellular properties of different calcium phosphate-bioactive glass composite chitosan-collagen (CaP@ChiCol) for bone scaffolds.
27184456	1	77	theme	matrix	540:545	arg1	phase					504:508	an organic phase	493:508	an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol)	493:560	In the present study, scaffolds for bone tissue engineering applications were made by immersing the inorganic phases of three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL) as a binder in an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol).
27184456	3	78	dep	in	659:660	arg1	vitro					662:666	vitro	662:666	vitro	662:666	The mechanical properties and in vitro growth of rat osteoblast-like UMR-106 cells were investigated.
27184456	1	79	dep	CaPs	313:316	arg1	CaPs					313:316	three different calcium phosphate (CaPs)	278:317	three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL)	278:476	In the present study, scaffolds for bone tissue engineering applications were made by immersing the inorganic phases of three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL) as a binder in an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol).
27184456	1	79	dep	CaPs	313:316	arg1	BCP					401:403	BCP	401:403	BCP	401:403	In the present study, scaffolds for bone tissue engineering applications were made by immersing the inorganic phases of three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL) as a binder in an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol).
27184456	1	79	dep	CaPs	313:316	arg1	phosphate					352:360	tricalcium phosphate	341:360	tricalcium phosphate (TCP)	341:366	In the present study, scaffolds for bone tissue engineering applications were made by immersing the inorganic phases of three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL) as a binder in an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol).
27184456	1	79	dep	CaPs	313:316	arg1	hydroxyapatite					320:333	hydroxyapatite	320:333	hydroxyapatite (HA)	320:338	In the present study, scaffolds for bone tissue engineering applications were made by immersing the inorganic phases of three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL) as a binder in an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol).
27184456	1	79	dep	CaPs	313:316	arg1	phosphate					390:398	biphasic calcium phosphate	373:398	biphasic calcium phosphate	373:398	In the present study, scaffolds for bone tissue engineering applications were made by immersing the inorganic phases of three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL) as a binder in an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol).
27184456	1	79	dep	CaPs	313:316	arg1	HA					336:337	HA	336:337	HA	336:337	In the present study, scaffolds for bone tissue engineering applications were made by immersing the inorganic phases of three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL) as a binder in an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol).
27184456	1	79	dep	CaPs	313:316	arg1	TCP					363:365	TCP	363:365	TCP	363:365	In the present study, scaffolds for bone tissue engineering applications were made by immersing the inorganic phases of three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL) as a binder in an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol).
27184456	0	80	theme	@	129:129	arg1	ChiCol					130:135	CaP@ChiCol	126:135	CaP@ChiCol	126:135	Physico-chemical and in vitro cellular properties of different calcium phosphate-bioactive glass composite chitosan-collagen (CaP@ChiCol) for bone scaffolds.
27184456	0	80	theme	@	129:129	arg1	chitosan-collagen					107:123	glass composite chitosan-collagen	91:123	glass composite chitosan-collagen (CaP@ChiCol) for bone scaffolds	91:155	Physico-chemical and in vitro cellular properties of different calcium phosphate-bioactive glass composite chitosan-collagen (CaP@ChiCol) for bone scaffolds.
27184456	13	81	theme	Part	1709:1712	arg1	B					1714:1714	Part B	1709:1714	J Biomed Mater Res Part B: Appl Biomater, 105B: 1758-1766, 2017.	1690:1753	J Biomed Mater Res Part B: Appl Biomater, 105B: 1758-1766, 2017.
27184456	12	82	dep	©	1659:1659	arg1	Inc.					1685:1688	Inc.	1685:1688	Inc.	1685:1688	© 2016 Wiley Periodicals, Inc.
27184456	6	83	theme	Compressive	968:978	arg1	modulus					980:986	Compressive modulus	968:986	Compressive modulus of 459.01 and 435.95 MPa with compressive strength of 22.73 and 17.89 MPa	968:1060	Compressive modulus of 459.01 and 435.95 MPa with compressive strength of 22.73 and 17.89 MPa were observed for the HABG@ChiCol and TCPBG@ChiCol composites, respectively.
27184456	1	84	with	glass	424:428	arg1	PCL					473:475	PCL	473:475	PCL	473:475	In the present study, scaffolds for bone tissue engineering applications were made by immersing the inorganic phases of three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL) as a binder in an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol).
27184456	1	84	with	glass	424:428	arg1	polycaprolactone					455:470	polycaprolactone	455:470	polycaprolactone (PCL)	455:476	In the present study, scaffolds for bone tissue engineering applications were made by immersing the inorganic phases of three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL) as a binder in an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol).
27184456	2	85	theme	Porous	563:568	arg1	scaffolds					570:578	Porous scaffolds	563:578	Porous scaffolds	563:578	Porous scaffolds were obtained by freeze drying the combinations.
27184456	9	86	theme	UMR-106	1439:1445	arg1	cells					1447:1451	UMR-106 cells	1439:1451	UMR-106 cells	1439:1451	To test its bioactivity, BCPBG@ChiCol was chosen for MTT and ALP assays on UMR-106 cells.
27184456	7	87	theme	osteoblast	1204:1213	arg1	attachment					1215:1224	osteoblast attachment	1204:1224	osteoblast attachment	1204:1224	In vitro cell availability and proliferation tests confirmed the osteoblast attachment and growth on the CaPBG@ChiCol surface.
27184456	1	88	theme	calcium	294:300	arg1	phosphate					390:398	biphasic calcium phosphate	373:398	biphasic calcium phosphate	373:398	In the present study, scaffolds for bone tissue engineering applications were made by immersing the inorganic phases of three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL) as a binder in an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol).
27184456	1	88	theme	calcium	294:300	arg1	CaPs					313:316	three different calcium phosphate (CaPs)	278:317	three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL)	278:476	In the present study, scaffolds for bone tissue engineering applications were made by immersing the inorganic phases of three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL) as a binder in an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol).
27184456	1	88	theme	calcium	294:300	arg1	hydroxyapatite					320:333	hydroxyapatite	320:333	hydroxyapatite (HA)	320:338	In the present study, scaffolds for bone tissue engineering applications were made by immersing the inorganic phases of three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL) as a binder in an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol).
27184456	1	88	theme	calcium	294:300	arg1	phosphate					352:360	tricalcium phosphate	341:360	tricalcium phosphate (TCP)	341:366	In the present study, scaffolds for bone tissue engineering applications were made by immersing the inorganic phases of three different calcium phosphate (CaPs) (hydroxyapatite (HA), tricalcium phosphate (TCP), and biphasic calcium phosphate (BCP)) mixing bioactive glass (15Ca:80Si:5P) (BG) with polycaprolactone (PCL) as a binder in an organic phase of chitosan/collagen (ChiCol) matrix (CaPBG@ChiCol).
27184456	10	89	theme	higher	1519:1524	arg1	activity					1530:1537	higher ALP activity	1519:1537	higher ALP activity	1519:1537	The results indicated that the UMR-106 cells were viable and had higher ALP activity as the culturing times were increased.
27184456	0	90	theme	different	53:61	arg1	calcium					63:69	different calcium	53:69	different calcium	53:69	Physico-chemical and in vitro cellular properties of different calcium phosphate-bioactive glass composite chitosan-collagen (CaP@ChiCol) for bone scaffolds.
27184456	6	91	with	modulus	980:986	arg1	strength					1030:1037	compressive strength	1018:1037	compressive strength of 22.73 and 17.89 MPa	1018:1060	Compressive modulus of 459.01 and 435.95 MPa with compressive strength of 22.73 and 17.89 MPa were observed for the HABG@ChiCol and TCPBG@ChiCol composites, respectively.
27184456	7	92	theme	proliferation	1170:1182	arg1	tests					1184:1188	In vitro cell availability and proliferation tests	1139:1188	In vitro cell availability and proliferation tests	1139:1188	In vitro cell availability and proliferation tests confirmed the osteoblast attachment and growth on the CaPBG@ChiCol surface.
27184456	0	93	dep	Physico-chemical	0:15	arg1	properties					39:48	cellular properties	30:48	cellular properties	30:48	Physico-chemical and in vitro cellular properties of different calcium phosphate-bioactive glass composite chitosan-collagen (CaP@ChiCol) for bone scaffolds.
27184456	7	94	from	attachment	1215:1224	arg1	surface					1257:1263	the CaPBG@ChiCol surface	1240:1263	the CaPBG@ChiCol surface	1240:1263	In vitro cell availability and proliferation tests confirmed the osteoblast attachment and growth on the CaPBG@ChiCol surface.
27184456	6	95	theme	435.95 MPa	1002:1011	arg1	modulus					980:986	Compressive modulus	968:986	Compressive modulus of 459.01 and 435.95 MPa with compressive strength of 22.73 and 17.89 MPa	968:1060	Compressive modulus of 459.01 and 435.95 MPa with compressive strength of 22.73 and 17.89 MPa were observed for the HABG@ChiCol and TCPBG@ChiCol composites, respectively.
27184456	7	96	from	growth	1230:1235	arg1	surface					1257:1263	the CaPBG@ChiCol surface	1240:1263	the CaPBG@ChiCol surface	1240:1263	In vitro cell availability and proliferation tests confirmed the osteoblast attachment and growth on the CaPBG@ChiCol surface.
27184456	8	97	theme	based	1316:1320	arg1	composite					1322:1330	the BCPBG based composite	1306:1330	the BCPBG based composite	1306:1330	Comparing the scaffolds, cells grown on the BCPBG based composite showed the higher cell density.
28384592	0	0	theme	Miscanthus	85:94	arg1	species					96:102	Miscanthus species	85:102	Miscanthus species	85:102	Changes in composition, cellulose degradability and biochemical methane potential of Miscanthus species during the growing season.
28384592	7	1	theme	harvest	936:942	arg1	time					944:947	the suitable harvest time	923:947	the suitable harvest time	923:947	This suggests that besides the biomass yield, it is justifiable to consider cellulose degradability when selecting the suitable harvest time for biofuels production from Miscanthus, especially in tropical and subtropical regions where Miscanthus can be harvested twice or more within a year.
28384592	7	2	theme	biomass	839:845	arg1	yield					847:851	the biomass yield	835:851	the biomass yield	835:851	This suggests that besides the biomass yield, it is justifiable to consider cellulose degradability when selecting the suitable harvest time for biofuels production from Miscanthus, especially in tropical and subtropical regions where Miscanthus can be harvested twice or more within a year.
28384592	1	3	theme	growing	366:372	arg1	season					374:379	their growing season	360:379	their growing season	360:379	The composition, cellulose degradability and biochemical methane potential (BMP) of M. sinensis, M. floridulus, Miscanthus×giganteus and M. lutarioriparius were investigated concomitantly at different growth/harvest times during their growing season.
28384592	4	4	theme	growth	529:534	arg1	time					536:539	the growth time	525:539	the growth time of 60days	525:549	At the growth time of 60days the BMPs ranged from 247.1 to 266.5mlg-1VS.
28384592	1	5	theme	Miscanthus×giganteus	243:262	arg1	potential					196:204	biochemical methane potential	176:204	biochemical methane potential (BMP)	176:210	The composition, cellulose degradability and biochemical methane potential (BMP) of M. sinensis, M. floridulus, Miscanthus×giganteus and M. lutarioriparius were investigated concomitantly at different growth/harvest times during their growing season.
28384592	1	5	theme	Miscanthus×giganteus	243:262	arg1	composition					135:145	The composition	131:145	The composition	131:145	The composition, cellulose degradability and biochemical methane potential (BMP) of M. sinensis, M. floridulus, Miscanthus×giganteus and M. lutarioriparius were investigated concomitantly at different growth/harvest times during their growing season.
28384592	1	5	theme	Miscanthus×giganteus	243:262	arg1	degradability					158:170	cellulose degradability	148:170	cellulose degradability	148:170	The composition, cellulose degradability and biochemical methane potential (BMP) of M. sinensis, M. floridulus, Miscanthus×giganteus and M. lutarioriparius were investigated concomitantly at different growth/harvest times during their growing season.
28384592	1	5	theme	Miscanthus×giganteus	243:262	arg1	BMP					207:209	BMP	207:209	BMP	207:209	The composition, cellulose degradability and biochemical methane potential (BMP) of M. sinensis, M. floridulus, Miscanthus×giganteus and M. lutarioriparius were investigated concomitantly at different growth/harvest times during their growing season.
28384592	0	6	from	Changes	0:6	arg1	composition					11:21	composition	11:21	composition	11:21	Changes in composition, cellulose degradability and biochemical methane potential of Miscanthus species during the growing season.
28384592	0	6	from	Changes	0:6	arg1	degradability					34:46	cellulose degradability	24:46	cellulose degradability	24:46	Changes in composition, cellulose degradability and biochemical methane potential of Miscanthus species during the growing season.
28384592	0	6	from	Changes	0:6	arg1	potential					72:80	biochemical methane potential	52:80	biochemical methane potential	52:80	Changes in composition, cellulose degradability and biochemical methane potential of Miscanthus species during the growing season.
28384592	7	7	from	regions	1029:1035	arg1	justifiable					860:870	justifiable	860:870	justifiable	860:870	This suggests that besides the biomass yield, it is justifiable to consider cellulose degradability when selecting the suitable harvest time for biofuels production from Miscanthus, especially in tropical and subtropical regions where Miscanthus can be harvested twice or more within a year.
28384592	0	8	theme	species	96:102	arg1	composition					11:21	composition	11:21	composition	11:21	Changes in composition, cellulose degradability and biochemical methane potential of Miscanthus species during the growing season.
28384592	0	8	theme	species	96:102	arg1	degradability					34:46	cellulose degradability	24:46	cellulose degradability	24:46	Changes in composition, cellulose degradability and biochemical methane potential of Miscanthus species during the growing season.
28384592	0	8	theme	species	96:102	arg1	potential					72:80	biochemical methane potential	52:80	biochemical methane potential	52:80	Changes in composition, cellulose degradability and biochemical methane potential of Miscanthus species during the growing season.
28384592	0	9	theme	growing	115:121	arg1	season					123:128	the growing season	111:128	the growing season	111:128	Changes in composition, cellulose degradability and biochemical methane potential of Miscanthus species during the growing season.
28384592	7	10	from	justifiable	860:870	arg1	regions					1029:1035	tropical and subtropical regions	1004:1035	tropical and subtropical regions where Miscanthus can be harvested twice or more within a year	1004:1097	This suggests that besides the biomass yield, it is justifiable to consider cellulose degradability when selecting the suitable harvest time for biofuels production from Miscanthus, especially in tropical and subtropical regions where Miscanthus can be harvested twice or more within a year.
28384592	5	11	theme	growth	598:603	arg1	time					605:608	growth time	598:608	growth time	598:608	As growth time was prolonged, the BMPs decreased by 11-35%.
28384592	1	12	theme	biochemical	176:186	arg1	potential					196:204	biochemical methane potential	176:204	biochemical methane potential (BMP)	176:210	The composition, cellulose degradability and biochemical methane potential (BMP) of M. sinensis, M. floridulus, Miscanthus×giganteus and M. lutarioriparius were investigated concomitantly at different growth/harvest times during their growing season.
28384592	1	12	theme	biochemical	176:186	arg1	BMP					207:209	BMP	207:209	BMP	207:209	The composition, cellulose degradability and biochemical methane potential (BMP) of M. sinensis, M. floridulus, Miscanthus×giganteus and M. lutarioriparius were investigated concomitantly at different growth/harvest times during their growing season.
28384592	6	13	theme	correlation	747:757	arg1	R2					773:774	R2	773:774	R2	773:774	For each species, the BMP was positively correlated to the cellulose degradability with the correlation coefficients (R2) ranging from 0.8055 to 0.9925.
28384592	6	13	theme	correlation	747:757	arg1	coefficients					759:770	the correlation coefficients	743:770	the correlation coefficients (R2) ranging from 0.8055 to 0.9925	743:805	For each species, the BMP was positively correlated to the cellulose degradability with the correlation coefficients (R2) ranging from 0.8055 to 0.9925.
28384592	1	14	theme	methane	188:194	arg1	potential					196:204	biochemical methane potential	176:204	biochemical methane potential (BMP)	176:210	The composition, cellulose degradability and biochemical methane potential (BMP) of M. sinensis, M. floridulus, Miscanthus×giganteus and M. lutarioriparius were investigated concomitantly at different growth/harvest times during their growing season.
28384592	1	14	theme	methane	188:194	arg1	BMP					207:209	BMP	207:209	BMP	207:209	The composition, cellulose degradability and biochemical methane potential (BMP) of M. sinensis, M. floridulus, Miscanthus×giganteus and M. lutarioriparius were investigated concomitantly at different growth/harvest times during their growing season.
28384592	2	15	theme	compositional	446:458	arg1	content					460:466	the compositional content	442:466	the compositional content	442:466	For all the four species, there was only a slight change in the compositional content.
28384592	7	16	theme	cellulose	884:892	arg1	degradability					894:906	cellulose degradability	884:906	cellulose degradability	884:906	This suggests that besides the biomass yield, it is justifiable to consider cellulose degradability when selecting the suitable harvest time for biofuels production from Miscanthus, especially in tropical and subtropical regions where Miscanthus can be harvested twice or more within a year.
28384592	1	17	theme	lutarioriparius	271:285	arg1	potential					196:204	biochemical methane potential	176:204	biochemical methane potential (BMP)	176:210	The composition, cellulose degradability and biochemical methane potential (BMP) of M. sinensis, M. floridulus, Miscanthus×giganteus and M. lutarioriparius were investigated concomitantly at different growth/harvest times during their growing season.
28384592	1	17	theme	lutarioriparius	271:285	arg1	composition					135:145	The composition	131:145	The composition	131:145	The composition, cellulose degradability and biochemical methane potential (BMP) of M. sinensis, M. floridulus, Miscanthus×giganteus and M. lutarioriparius were investigated concomitantly at different growth/harvest times during their growing season.
28384592	1	17	theme	lutarioriparius	271:285	arg1	degradability					158:170	cellulose degradability	148:170	cellulose degradability	148:170	The composition, cellulose degradability and biochemical methane potential (BMP) of M. sinensis, M. floridulus, Miscanthus×giganteus and M. lutarioriparius were investigated concomitantly at different growth/harvest times during their growing season.
28384592	1	17	theme	lutarioriparius	271:285	arg1	BMP					207:209	BMP	207:209	BMP	207:209	The composition, cellulose degradability and biochemical methane potential (BMP) of M. sinensis, M. floridulus, Miscanthus×giganteus and M. lutarioriparius were investigated concomitantly at different growth/harvest times during their growing season.
28384592	3	18	from	change	496:501	arg1	values					514:519	the BMP values	506:519	the BMP values	506:519	Meanwhile there was a huge change in the BMP values.
28384592	2	19	theme	slight	425:430	arg1	change					432:437	only a slight change	418:437	only a slight change	418:437	For all the four species, there was only a slight change in the compositional content.
28384592	4	20	theme	60days	544:549	arg1	time					536:539	the growth time	525:539	the growth time of 60days	525:549	At the growth time of 60days the BMPs ranged from 247.1 to 266.5mlg-1VS.
28384592	0	21	theme	cellulose	24:32	arg1	degradability					34:46	cellulose degradability	24:46	cellulose degradability	24:46	Changes in composition, cellulose degradability and biochemical methane potential of Miscanthus species during the growing season.
28384592	7	22	theme	subtropical	1017:1027	arg1	regions					1029:1035	tropical and subtropical regions	1004:1035	tropical and subtropical regions where Miscanthus can be harvested twice or more within a year	1004:1097	This suggests that besides the biomass yield, it is justifiable to consider cellulose degradability when selecting the suitable harvest time for biofuels production from Miscanthus, especially in tropical and subtropical regions where Miscanthus can be harvested twice or more within a year.
28384592	6	23	theme	cellulose	714:722	arg1	degradability					724:736	the cellulose degradability	710:736	the cellulose degradability	710:736	For each species, the BMP was positively correlated to the cellulose degradability with the correlation coefficients (R2) ranging from 0.8055 to 0.9925.
28384592	7	24	from	Miscanthus	978:987	arg1	production					962:971	biofuels production	953:971	biofuels production from Miscanthus	953:987	This suggests that besides the biomass yield, it is justifiable to consider cellulose degradability when selecting the suitable harvest time for biofuels production from Miscanthus, especially in tropical and subtropical regions where Miscanthus can be harvested twice or more within a year.
28384592	0	25	theme	biochemical	52:62	arg1	potential					72:80	biochemical methane potential	52:80	biochemical methane potential	52:80	Changes in composition, cellulose degradability and biochemical methane potential of Miscanthus species during the growing season.
28384592	1	26	theme	different	322:330	arg1	times					347:351	different growth/harvest times	322:351	different growth/harvest times	322:351	The composition, cellulose degradability and biochemical methane potential (BMP) of M. sinensis, M. floridulus, Miscanthus×giganteus and M. lutarioriparius were investigated concomitantly at different growth/harvest times during their growing season.
28384592	3	27	theme	huge	491:494	arg1	change					496:501	a huge change	489:501	a huge change in the BMP values	489:519	Meanwhile there was a huge change in the BMP values.
28384592	1	28	theme	growth/harvest	332:345	arg1	times					347:351	different growth/harvest times	322:351	different growth/harvest times	322:351	The composition, cellulose degradability and biochemical methane potential (BMP) of M. sinensis, M. floridulus, Miscanthus×giganteus and M. lutarioriparius were investigated concomitantly at different growth/harvest times during their growing season.
28384592	7	29	theme	tropical	1004:1011	arg1	regions					1029:1035	tropical and subtropical regions	1004:1035	tropical and subtropical regions where Miscanthus can be harvested twice or more within a year	1004:1097	This suggests that besides the biomass yield, it is justifiable to consider cellulose degradability when selecting the suitable harvest time for biofuels production from Miscanthus, especially in tropical and subtropical regions where Miscanthus can be harvested twice or more within a year.
28384592	7	30	theme	biofuels	953:960	arg1	production					962:971	biofuels production	953:971	biofuels production from Miscanthus	953:987	This suggests that besides the biomass yield, it is justifiable to consider cellulose degradability when selecting the suitable harvest time for biofuels production from Miscanthus, especially in tropical and subtropical regions where Miscanthus can be harvested twice or more within a year.
28384592	6	31	dep	0.9925	800:805	arg1	to					797:798	to	797:798	to	797:798	For each species, the BMP was positively correlated to the cellulose degradability with the correlation coefficients (R2) ranging from 0.8055 to 0.9925.
28384592	1	32	theme	sinensis	218:225	arg1	potential					196:204	biochemical methane potential	176:204	biochemical methane potential (BMP)	176:210	The composition, cellulose degradability and biochemical methane potential (BMP) of M. sinensis, M. floridulus, Miscanthus×giganteus and M. lutarioriparius were investigated concomitantly at different growth/harvest times during their growing season.
28384592	1	32	theme	sinensis	218:225	arg1	composition					135:145	The composition	131:145	The composition	131:145	The composition, cellulose degradability and biochemical methane potential (BMP) of M. sinensis, M. floridulus, Miscanthus×giganteus and M. lutarioriparius were investigated concomitantly at different growth/harvest times during their growing season.
28384592	1	32	theme	sinensis	218:225	arg1	degradability					158:170	cellulose degradability	148:170	cellulose degradability	148:170	The composition, cellulose degradability and biochemical methane potential (BMP) of M. sinensis, M. floridulus, Miscanthus×giganteus and M. lutarioriparius were investigated concomitantly at different growth/harvest times during their growing season.
28384592	1	32	theme	sinensis	218:225	arg1	BMP					207:209	BMP	207:209	BMP	207:209	The composition, cellulose degradability and biochemical methane potential (BMP) of M. sinensis, M. floridulus, Miscanthus×giganteus and M. lutarioriparius were investigated concomitantly at different growth/harvest times during their growing season.
28384592	4	33	dep	266.5mlg-1VS	581:592	arg1	to					578:579	to	578:579	to	578:579	At the growth time of 60days the BMPs ranged from 247.1 to 266.5mlg-1VS.
28384592	3	34	dep	Meanwhile	469:477	arg1	there					479:483	there	479:483	there	479:483	Meanwhile there was a huge change in the BMP values.
28384592	3	35	theme	BMP	510:512	arg1	values					514:519	the BMP values	506:519	the BMP values	506:519	Meanwhile there was a huge change in the BMP values.
28384592	0	36	theme	methane	64:70	arg1	potential					72:80	biochemical methane potential	52:80	biochemical methane potential	52:80	Changes in composition, cellulose degradability and biochemical methane potential of Miscanthus species during the growing season.
28384592	7	37	theme	suitable	927:934	arg1	time					944:947	the suitable harvest time	923:947	the suitable harvest time	923:947	This suggests that besides the biomass yield, it is justifiable to consider cellulose degradability when selecting the suitable harvest time for biofuels production from Miscanthus, especially in tropical and subtropical regions where Miscanthus can be harvested twice or more within a year.
28384592	1	38	theme	cellulose	148:156	arg1	degradability					158:170	cellulose degradability	148:170	cellulose degradability	148:170	The composition, cellulose degradability and biochemical methane potential (BMP) of M. sinensis, M. floridulus, Miscanthus×giganteus and M. lutarioriparius were investigated concomitantly at different growth/harvest times during their growing season.
28384592	1	39	theme	floridulus	231:240	arg1	potential					196:204	biochemical methane potential	176:204	biochemical methane potential (BMP)	176:210	The composition, cellulose degradability and biochemical methane potential (BMP) of M. sinensis, M. floridulus, Miscanthus×giganteus and M. lutarioriparius were investigated concomitantly at different growth/harvest times during their growing season.
28384592	1	39	theme	floridulus	231:240	arg1	composition					135:145	The composition	131:145	The composition	131:145	The composition, cellulose degradability and biochemical methane potential (BMP) of M. sinensis, M. floridulus, Miscanthus×giganteus and M. lutarioriparius were investigated concomitantly at different growth/harvest times during their growing season.
28384592	1	39	theme	floridulus	231:240	arg1	degradability					158:170	cellulose degradability	148:170	cellulose degradability	148:170	The composition, cellulose degradability and biochemical methane potential (BMP) of M. sinensis, M. floridulus, Miscanthus×giganteus and M. lutarioriparius were investigated concomitantly at different growth/harvest times during their growing season.
28384592	1	39	theme	floridulus	231:240	arg1	BMP					207:209	BMP	207:209	BMP	207:209	The composition, cellulose degradability and biochemical methane potential (BMP) of M. sinensis, M. floridulus, Miscanthus×giganteus and M. lutarioriparius were investigated concomitantly at different growth/harvest times during their growing season.
27979726	9	0	from	applications	1163:1174	arg1	industries					1179:1188	industries	1179:1188	industries	1179:1188	All the above findings on BtqEPS paves way to find novel insights of its potential applications in industries.
27979726	8	1	theme	TGA	880:882	arg1	curve					884:888	TGA curve	880:888	TGA curve	880:888	TGA curve indicated the degradation temperature (Td) of 239.72°C. Furthermore, BtqEPS exhibited antioxidant effects by scavenging reactive oxygen species (ROS) and demonstrated strong reducing power.
27979726	9	2	from	findings	1094:1101	arg1	BtqEPS					1106:1111	BtqEPS	1106:1111	BtqEPS	1106:1111	All the above findings on BtqEPS paves way to find novel insights of its potential applications in industries.
27979726	4	3	theme	linkages	589:596	arg1	presence					531:538	the presence	527:538	the presence of α and β-d-(+)-glucose residues and glycosidic linkages	527:596	1H NMR and 13C NMR spectra analysis showed the presence of α and β-d-(+)-glucose residues and glycosidic linkages.
27979726	5	4	theme	SEM	704:706	arg1	analysis					716:723	SEM and EDX analysis	704:723	analysis	716:723	The fibrous, porous nature and elemental composition (C, O, N, Cl, Na, P, S) of BtqEPS was inferred from SEM and EDX analysis.
27979726	4	5	theme	α	543:543	arg1	residues					565:572	α and β-d-(+)-glucose residues	543:572	α and β-d-(+)-glucose residues	543:572	1H NMR and 13C NMR spectra analysis showed the presence of α and β-d-(+)-glucose residues and glycosidic linkages.
27979726	8	6	theme	239.72°C.	936:944	arg1	Td					929:930	Td	929:930	Td	929:930	TGA curve indicated the degradation temperature (Td) of 239.72°C. Furthermore, BtqEPS exhibited antioxidant effects by scavenging reactive oxygen species (ROS) and demonstrated strong reducing power.
27979726	8	6	theme	239.72°C.	936:944	arg1	temperature					916:926	the degradation temperature	900:926	the degradation temperature (Td) of 239.72°C.	900:944	TGA curve indicated the degradation temperature (Td) of 239.72°C. Furthermore, BtqEPS exhibited antioxidant effects by scavenging reactive oxygen species (ROS) and demonstrated strong reducing power.
27979726	5	7	theme	fibrous	603:609	arg1	nature					619:624	The fibrous, porous nature	599:624	nature	619:624	The fibrous, porous nature and elemental composition (C, O, N, Cl, Na, P, S) of BtqEPS was inferred from SEM and EDX analysis.
27979726	7	8	theme	XRD	794:796	arg1	analysis					798:805	XRD analysis	794:805	XRD analysis	794:805	XRD analysis confirmed the amorphous nature of BtqEPS with 15.6% crystallinity index.
27979726	4	9	theme	glycosidic	578:587	arg1	linkages					589:596	glycosidic linkages	578:596	glycosidic linkages	578:596	1H NMR and 13C NMR spectra analysis showed the presence of α and β-d-(+)-glucose residues and glycosidic linkages.
27979726	9	10	theme	above	1088:1092	arg1	findings					1094:1101	All the above findings	1080:1101	All the above findings on BtqEPS	1080:1111	All the above findings on BtqEPS paves way to find novel insights of its potential applications in industries.
27979726	4	11	theme	spectra	503:509	arg1	analysis					511:518	1H NMR and 13C NMR spectra analysis	484:518	1H NMR and 13C NMR spectra analysis	484:518	1H NMR and 13C NMR spectra analysis showed the presence of α and β-d-(+)-glucose residues and glycosidic linkages.
27979726	5	12	theme	porous	612:617	arg1	nature					619:624	The fibrous, porous nature	599:624	nature	619:624	The fibrous, porous nature and elemental composition (C, O, N, Cl, Na, P, S) of BtqEPS was inferred from SEM and EDX analysis.
27979726	9	13	from	insights	1137:1144	arg1	industries					1179:1188	industries	1179:1188	industries	1179:1188	All the above findings on BtqEPS paves way to find novel insights of its potential applications in industries.
27979726	4	14	theme	NMR	499:501	arg1	spectra					503:509	13C NMR spectra	495:509	13C NMR spectra	495:509	1H NMR and 13C NMR spectra analysis showed the presence of α and β-d-(+)-glucose residues and glycosidic linkages.
27979726	8	15	theme	antioxidant	976:986	arg1	effects					988:994	antioxidant effects	976:994	antioxidant effects	976:994	TGA curve indicated the degradation temperature (Td) of 239.72°C. Furthermore, BtqEPS exhibited antioxidant effects by scavenging reactive oxygen species (ROS) and demonstrated strong reducing power.
27979726	8	16	theme	degradation	904:914	arg1	Td					929:930	Td	929:930	Td	929:930	TGA curve indicated the degradation temperature (Td) of 239.72°C. Furthermore, BtqEPS exhibited antioxidant effects by scavenging reactive oxygen species (ROS) and demonstrated strong reducing power.
27979726	8	16	theme	degradation	904:914	arg1	temperature					916:926	the degradation temperature	900:926	the degradation temperature (Td) of 239.72°C.	900:944	TGA curve indicated the degradation temperature (Td) of 239.72°C. Furthermore, BtqEPS exhibited antioxidant effects by scavenging reactive oxygen species (ROS) and demonstrated strong reducing power.
27979726	3	17	theme	groups	476:481	arg1	presence					442:449	the presence	438:449	the presence of carboxyl and hydroxyl groups	438:481	FT-IR spectroscopy confirmed the presence of carboxyl and hydroxyl groups.
27979726	5	18	theme	BtqEPS	679:684	arg1	composition					640:650	elemental composition	630:650	elemental composition	630:650	The fibrous, porous nature and elemental composition (C, O, N, Cl, Na, P, S) of BtqEPS was inferred from SEM and EDX analysis.
27979726	5	18	theme	BtqEPS	679:684	arg1	nature					619:624	The fibrous, porous nature	599:624	nature	619:624	The fibrous, porous nature and elemental composition (C, O, N, Cl, Na, P, S) of BtqEPS was inferred from SEM and EDX analysis.
27979726	5	19	theme	elemental	630:638	arg1	composition					640:650	elemental composition	630:650	elemental composition	630:650	The fibrous, porous nature and elemental composition (C, O, N, Cl, Na, P, S) of BtqEPS was inferred from SEM and EDX analysis.
27979726	7	20	theme	%	857:857	arg1	index					873:877	15.6% crystallinity index	853:877	15.6% crystallinity index	853:877	XRD analysis confirmed the amorphous nature of BtqEPS with 15.6% crystallinity index.
27979726	8	21	theme	reducing	1064:1071	arg1	power					1073:1077	strong reducing power	1057:1077	strong reducing power	1057:1077	TGA curve indicated the degradation temperature (Td) of 239.72°C. Furthermore, BtqEPS exhibited antioxidant effects by scavenging reactive oxygen species (ROS) and demonstrated strong reducing power.
27979726	7	22	theme	crystallinity	859:871	arg1	index					873:877	15.6% crystallinity index	853:877	15.6% crystallinity index	853:877	XRD analysis confirmed the amorphous nature of BtqEPS with 15.6% crystallinity index.
27979726	0	23	dep	FR9	107:109	arg1	isolated					111:118	isolated	111:118	FR9 isolated from chicken	107:131	Physiochemical and biological characterization of novel exopolysaccharide produced by Bacillus tequilensis FR9 isolated from chicken.
27979726	0	24	theme	Physiochemical	0:13	arg1	characterization					30:45	Physiochemical and biological characterization	0:45	Physiochemical and biological characterization of novel exopolysaccharide produced by Bacillus tequilensis	0:105	Physiochemical and biological characterization of novel exopolysaccharide produced by Bacillus tequilensis FR9 isolated from chicken.
27979726	4	25	theme	-glucose	556:563	arg1	residues					565:572	α and β-d-(+)-glucose residues	543:572	α and β-d-(+)-glucose residues	543:572	1H NMR and 13C NMR spectra analysis showed the presence of α and β-d-(+)-glucose residues and glycosidic linkages.
27979726	3	26	theme	hydroxyl	467:474	arg1	groups					476:481	carboxyl and hydroxyl groups	454:481	groups	476:481	FT-IR spectroscopy confirmed the presence of carboxyl and hydroxyl groups.
27979726	8	27	theme	reactive	1010:1017	arg1	ROS					1035:1037	ROS	1035:1037	ROS	1035:1037	TGA curve indicated the degradation temperature (Td) of 239.72°C. Furthermore, BtqEPS exhibited antioxidant effects by scavenging reactive oxygen species (ROS) and demonstrated strong reducing power.
27979726	8	27	theme	reactive	1010:1017	arg1	species					1026:1032	scavenging reactive oxygen species	999:1032	scavenging reactive oxygen species (ROS)	999:1038	TGA curve indicated the degradation temperature (Td) of 239.72°C. Furthermore, BtqEPS exhibited antioxidant effects by scavenging reactive oxygen species (ROS) and demonstrated strong reducing power.
27979726	7	28	theme	15.6	853:856	arg1	%					857:857	%	857:857	%	857:857	XRD analysis confirmed the amorphous nature of BtqEPS with 15.6% crystallinity index.
27979726	2	29	theme	monosaccharides	332:346	arg1	existence					314:322	the existence	310:322	the existence of five monosaccharides including glucose, galactose, mannose, arabinose and xylose	310:406	HPLC analysis revealed the existence of five monosaccharides including glucose, galactose, mannose, arabinose and xylose.
27979726	3	30	theme	FT-IR	409:413	arg1	spectroscopy					415:426	FT-IR spectroscopy	409:426	FT-IR spectroscopy	409:426	FT-IR spectroscopy confirmed the presence of carboxyl and hydroxyl groups.
27979726	8	31	theme	scavenging	999:1008	arg1	ROS					1035:1037	ROS	1035:1037	ROS	1035:1037	TGA curve indicated the degradation temperature (Td) of 239.72°C. Furthermore, BtqEPS exhibited antioxidant effects by scavenging reactive oxygen species (ROS) and demonstrated strong reducing power.
27979726	8	31	theme	scavenging	999:1008	arg1	species					1026:1032	scavenging reactive oxygen species	999:1032	scavenging reactive oxygen species (ROS)	999:1038	TGA curve indicated the degradation temperature (Td) of 239.72°C. Furthermore, BtqEPS exhibited antioxidant effects by scavenging reactive oxygen species (ROS) and demonstrated strong reducing power.
27979726	4	32	theme	NMR	487:489	arg1	analysis					511:518	1H NMR and 13C NMR spectra analysis	484:518	1H NMR and 13C NMR spectra analysis	484:518	1H NMR and 13C NMR spectra analysis showed the presence of α and β-d-(+)-glucose residues and glycosidic linkages.
27979726	0	33	theme	novel	50:54	arg1	exopolysaccharide					56:72	novel exopolysaccharide	50:72	novel exopolysaccharide produced by Bacillus tequilensis	50:105	Physiochemical and biological characterization of novel exopolysaccharide produced by Bacillus tequilensis FR9 isolated from chicken.
27979726	4	34	theme	residues	565:572	arg1	presence					531:538	the presence	527:538	the presence of α and β-d-(+)-glucose residues and glycosidic linkages	527:596	1H NMR and 13C NMR spectra analysis showed the presence of α and β-d-(+)-glucose residues and glycosidic linkages.
27979726	4	35	theme	1H	484:485	arg1	NMR					487:489	1H NMR	484:489	1H NMR	484:489	1H NMR and 13C NMR spectra analysis showed the presence of α and β-d-(+)-glucose residues and glycosidic linkages.
27979726	3	36	theme	carboxyl	454:461	arg1	groups					476:481	carboxyl and hydroxyl groups	454:481	groups	476:481	FT-IR spectroscopy confirmed the presence of carboxyl and hydroxyl groups.
27979726	4	37	theme	β-d-	549:552	arg1	residues					565:572	α and β-d-(+)-glucose residues	543:572	α and β-d-(+)-glucose residues	543:572	1H NMR and 13C NMR spectra analysis showed the presence of α and β-d-(+)-glucose residues and glycosidic linkages.
27979726	5	38	theme	EDX	712:714	arg1	analysis					716:723	SEM and EDX analysis	704:723	analysis	716:723	The fibrous, porous nature and elemental composition (C, O, N, Cl, Na, P, S) of BtqEPS was inferred from SEM and EDX analysis.
27979726	4	39	theme	13C	495:497	arg1	spectra					503:509	13C NMR spectra	495:509	13C NMR spectra	495:509	1H NMR and 13C NMR spectra analysis showed the presence of α and β-d-(+)-glucose residues and glycosidic linkages.
27979726	1	40	theme	DEAE-Sepharose	237:250	arg1	column					279:284	DEAE-Sepharose and phenyl sepharose CL-6B column	237:284	column	279:284	Hetero-exopolysaccharide secreted by Bacillus tequilensis FR9 (BtqEPS), was produced and fractioned on DEAE-Sepharose and phenyl sepharose CL-6B column.
27979726	9	41	theme	potential	1153:1161	arg1	applications					1163:1174	its potential applications	1149:1174	its potential applications in industries	1149:1188	All the above findings on BtqEPS paves way to find novel insights of its potential applications in industries.
27979726	0	42	theme	exopolysaccharide	56:72	arg1	characterization					30:45	Physiochemical and biological characterization	0:45	Physiochemical and biological characterization of novel exopolysaccharide produced by Bacillus tequilensis	0:105	Physiochemical and biological characterization of novel exopolysaccharide produced by Bacillus tequilensis FR9 isolated from chicken.
27979726	9	43	theme	applications	1163:1174	arg1	insights					1137:1144	novel insights	1131:1144	novel insights of its potential applications in industries	1131:1188	All the above findings on BtqEPS paves way to find novel insights of its potential applications in industries.
27979726	9	44	from	industries	1179:1188	arg1	insights					1137:1144	novel insights	1131:1144	novel insights of its potential applications in industries	1131:1188	All the above findings on BtqEPS paves way to find novel insights of its potential applications in industries.
27979726	9	45	theme	novel	1131:1135	arg1	insights					1137:1144	novel insights	1131:1144	novel insights of its potential applications in industries	1131:1188	All the above findings on BtqEPS paves way to find novel insights of its potential applications in industries.
27979726	6	46	theme	BtqEPS	765:770	arg1	micro-structure					746:760	the micro-structure	742:760	the micro-structure of BtqEPS	742:770	AFM proved that the micro-structure of BtqEPS is compact and rough.
27979726	6	46	theme	BtqEPS	765:770	arg1	compact					775:781	compact	775:781	compact	775:781	AFM proved that the micro-structure of BtqEPS is compact and rough.
27979726	5	47	dep	nature	619:624	arg1	P					670:670	P	670:670	P	670:670	The fibrous, porous nature and elemental composition (C, O, N, Cl, Na, P, S) of BtqEPS was inferred from SEM and EDX analysis.
27979726	5	47	dep	nature	619:624	arg1	N					659:659	N	659:659	N	659:659	The fibrous, porous nature and elemental composition (C, O, N, Cl, Na, P, S) of BtqEPS was inferred from SEM and EDX analysis.
27979726	5	47	dep	nature	619:624	arg1	Cl					662:663	Cl	662:663	Cl	662:663	The fibrous, porous nature and elemental composition (C, O, N, Cl, Na, P, S) of BtqEPS was inferred from SEM and EDX analysis.
27979726	5	47	dep	nature	619:624	arg1	S					673:673	S	673:673	S	673:673	The fibrous, porous nature and elemental composition (C, O, N, Cl, Na, P, S) of BtqEPS was inferred from SEM and EDX analysis.
27979726	5	47	dep	nature	619:624	arg1	C					653:653	C	653:653	C	653:653	The fibrous, porous nature and elemental composition (C, O, N, Cl, Na, P, S) of BtqEPS was inferred from SEM and EDX analysis.
27979726	5	47	dep	nature	619:624	arg1	O					656:656	O	656:656	O	656:656	The fibrous, porous nature and elemental composition (C, O, N, Cl, Na, P, S) of BtqEPS was inferred from SEM and EDX analysis.
27979726	5	47	dep	nature	619:624	arg1	Na					666:667	Na	666:667	Na	666:667	The fibrous, porous nature and elemental composition (C, O, N, Cl, Na, P, S) of BtqEPS was inferred from SEM and EDX analysis.
27979726	2	48	theme	HPLC	287:290	arg1	analysis					292:299	HPLC analysis	287:299	HPLC analysis	287:299	HPLC analysis revealed the existence of five monosaccharides including glucose, galactose, mannose, arabinose and xylose.
27979726	1	49	theme	Bacillus	171:178	arg1	FR9					192:194	Bacillus tequilensis FR9	171:194	Bacillus tequilensis FR9 (BtqEPS)	171:203	Hetero-exopolysaccharide secreted by Bacillus tequilensis FR9 (BtqEPS), was produced and fractioned on DEAE-Sepharose and phenyl sepharose CL-6B column.
27979726	1	49	theme	Bacillus	171:178	arg1	BtqEPS					197:202	BtqEPS	197:202	BtqEPS	197:202	Hetero-exopolysaccharide secreted by Bacillus tequilensis FR9 (BtqEPS), was produced and fractioned on DEAE-Sepharose and phenyl sepharose CL-6B column.
27979726	7	50	theme	amorphous	821:829	arg1	nature					831:836	the amorphous nature	817:836	the amorphous nature of BtqEPS	817:846	XRD analysis confirmed the amorphous nature of BtqEPS with 15.6% crystallinity index.
27979726	1	51	theme	phenyl	256:261	arg1	column					279:284	DEAE-Sepharose and phenyl sepharose CL-6B column	237:284	column	279:284	Hetero-exopolysaccharide secreted by Bacillus tequilensis FR9 (BtqEPS), was produced and fractioned on DEAE-Sepharose and phenyl sepharose CL-6B column.
27979726	8	52	theme	strong	1057:1062	arg1	power					1073:1077	strong reducing power	1057:1077	strong reducing power	1057:1077	TGA curve indicated the degradation temperature (Td) of 239.72°C. Furthermore, BtqEPS exhibited antioxidant effects by scavenging reactive oxygen species (ROS) and demonstrated strong reducing power.
27979726	1	53	theme	tequilensis	180:190	arg1	FR9					192:194	Bacillus tequilensis FR9	171:194	Bacillus tequilensis FR9 (BtqEPS)	171:203	Hetero-exopolysaccharide secreted by Bacillus tequilensis FR9 (BtqEPS), was produced and fractioned on DEAE-Sepharose and phenyl sepharose CL-6B column.
27979726	1	53	theme	tequilensis	180:190	arg1	BtqEPS					197:202	BtqEPS	197:202	BtqEPS	197:202	Hetero-exopolysaccharide secreted by Bacillus tequilensis FR9 (BtqEPS), was produced and fractioned on DEAE-Sepharose and phenyl sepharose CL-6B column.
27979726	7	54	theme	BtqEPS	841:846	arg1	nature					831:836	the amorphous nature	817:836	the amorphous nature of BtqEPS	817:846	XRD analysis confirmed the amorphous nature of BtqEPS with 15.6% crystallinity index.
27979726	1	55	theme	sepharose	263:271	arg1	column					279:284	DEAE-Sepharose and phenyl sepharose CL-6B column	237:284	column	279:284	Hetero-exopolysaccharide secreted by Bacillus tequilensis FR9 (BtqEPS), was produced and fractioned on DEAE-Sepharose and phenyl sepharose CL-6B column.
27979726	0	56	theme	biological	19:28	arg1	characterization					30:45	Physiochemical and biological characterization	0:45	Physiochemical and biological characterization of novel exopolysaccharide produced by Bacillus tequilensis	0:105	Physiochemical and biological characterization of novel exopolysaccharide produced by Bacillus tequilensis FR9 isolated from chicken.
27979726	8	57	theme	oxygen	1019:1024	arg1	ROS					1035:1037	ROS	1035:1037	ROS	1035:1037	TGA curve indicated the degradation temperature (Td) of 239.72°C. Furthermore, BtqEPS exhibited antioxidant effects by scavenging reactive oxygen species (ROS) and demonstrated strong reducing power.
27979726	8	57	theme	oxygen	1019:1024	arg1	species					1026:1032	scavenging reactive oxygen species	999:1032	scavenging reactive oxygen species (ROS)	999:1038	TGA curve indicated the degradation temperature (Td) of 239.72°C. Furthermore, BtqEPS exhibited antioxidant effects by scavenging reactive oxygen species (ROS) and demonstrated strong reducing power.
27979726	1	58	theme	CL-6B	273:277	arg1	column					279:284	DEAE-Sepharose and phenyl sepharose CL-6B column	237:284	column	279:284	Hetero-exopolysaccharide secreted by Bacillus tequilensis FR9 (BtqEPS), was produced and fractioned on DEAE-Sepharose and phenyl sepharose CL-6B column.
26267164	0	0	theme	strains	97:103	arg1	properties					53:62	functional properties	42:62	functional properties of some Lactobacillus salivarius strains	42:103	Impact of exopolysaccharide production on functional properties of some Lactobacillus salivarius strains.
26267164	7	1	theme	strains	1028:1034	arg1	properties					1000:1009	the functional properties	985:1009	the functional properties of L. salivarius strains	985:1034	This study showed that strain-specific properties can determine the functional properties of L. salivarius strains, and the interference of these properties might be crucial for final selection of these strains for technological purposes.
26267164	0	2	theme	salivarius	86:95	arg1	strains					97:103	some Lactobacillus salivarius strains	67:103	some Lactobacillus salivarius strains	67:103	Impact of exopolysaccharide production on functional properties of some Lactobacillus salivarius strains.
26267164	4	3	theme	medium	530:535	arg1	dependant					549:557	medium composition dependant	530:557	medium composition dependant	530:557	Biofilm formation was found to be medium composition dependant, and there was a negative correlation with biofilm formation and EPS production.
26267164	5	4	theme	L.	688:689	arg1	strains					702:708	L. salivarius strains	688:708	L. salivarius strains	688:708	Autoaggregation properties and coaggregation of L. salivarius strains with chicken pathogens were appeared to be specific at strain level.
26267164	2	5	theme	p-gtf	366:370	arg1	gene					372:375	p-gtf gene	366:375	p-gtf gene	366:375	Detection of genes responsible for exopolysaccharide (EPS) production revealed that all strains harbored a dextransucrase gene, but p-gtf gene was only detected in strain E4.
26267164	6	6	theme	EPS	898:900	arg1	levels					913:918	EPS production levels	898:918	EPS production levels	898:918	An increment in bacterial adhesion to chicken gut explants was observed in L. salivarius strains with the reduction in EPS production levels.
26267164	6	7	located	observed	842:849	arg1	strains					868:874	L. salivarius strains	854:874	L. salivarius strains with the reduction in EPS production levels	854:918	An increment in bacterial adhesion to chicken gut explants was observed in L. salivarius strains with the reduction in EPS production levels.
26267164	6	7	located	observed	842:849	arg2	increment					782:790	An increment	779:790	An increment in bacterial adhesion to chicken gut explants	779:836	An increment in bacterial adhesion to chicken gut explants was observed in L. salivarius strains with the reduction in EPS production levels.
26267164	0	8	from	Impact	0:5	arg1	properties					53:62	functional properties	42:62	functional properties of some Lactobacillus salivarius strains	42:103	Impact of exopolysaccharide production on functional properties of some Lactobacillus salivarius strains.
26267164	5	9	theme	salivarius	691:700	arg1	strains					702:708	L. salivarius strains	688:708	L. salivarius strains	688:708	Autoaggregation properties and coaggregation of L. salivarius strains with chicken pathogens were appeared to be specific at strain level.
26267164	7	10	theme	L.	1014:1015	arg1	strains					1028:1034	L. salivarius strains	1014:1034	L. salivarius strains	1014:1034	This study showed that strain-specific properties can determine the functional properties of L. salivarius strains, and the interference of these properties might be crucial for final selection of these strains for technological purposes.
26267164	5	11	theme	strain	765:770	arg1	level					772:776	strain level	765:776	strain level	765:776	Autoaggregation properties and coaggregation of L. salivarius strains with chicken pathogens were appeared to be specific at strain level.
26267164	4	12	theme	Biofilm	496:502	arg1	formation					504:512	Biofilm formation	496:512	Biofilm formation	496:512	Biofilm formation was found to be medium composition dependant, and there was a negative correlation with biofilm formation and EPS production.
26267164	5	13	theme	strains	702:708	arg1	coaggregation					671:683	coaggregation	671:683	coaggregation of L. salivarius strains with chicken pathogens	671:731	Autoaggregation properties and coaggregation of L. salivarius strains with chicken pathogens were appeared to be specific at strain level.
26267164	5	13	theme	strains	702:708	arg1	properties					656:665	Autoaggregation properties	640:665	Autoaggregation properties	640:665	Autoaggregation properties and coaggregation of L. salivarius strains with chicken pathogens were appeared to be specific at strain level.
26267164	7	14	theme	functional	989:998	arg1	properties					1000:1009	the functional properties	985:1009	the functional properties of L. salivarius strains	985:1034	This study showed that strain-specific properties can determine the functional properties of L. salivarius strains, and the interference of these properties might be crucial for final selection of these strains for technological purposes.
26267164	4	15	theme	biofilm	602:608	arg1	formation					610:618	biofilm formation	602:618	biofilm formation	602:618	Biofilm formation was found to be medium composition dependant, and there was a negative correlation with biofilm formation and EPS production.
26267164	5	16	theme	Autoaggregation	640:654	arg1	properties					656:665	Autoaggregation properties	640:665	Autoaggregation properties	640:665	Autoaggregation properties and coaggregation of L. salivarius strains with chicken pathogens were appeared to be specific at strain level.
26267164	3	17	theme	production	425:434	arg1	levels					436:441	EPS production levels	421:441	EPS production levels	421:441	Analysis of EPS production levels showed significant alterations among strains tested.
26267164	1	18	theme	Lactobacillus	172:184	arg1	strains					197:203	Lactobacillus salivarius strains	172:203	Lactobacillus salivarius strains isolated from chicken feces	172:231	The aim of this work was to characterize functional properties of Lactobacillus salivarius strains isolated from chicken feces.
26267164	7	19	theme	technological	1136:1148	arg1	purposes					1150:1157	technological purposes	1136:1157	technological purposes	1136:1157	This study showed that strain-specific properties can determine the functional properties of L. salivarius strains, and the interference of these properties might be crucial for final selection of these strains for technological purposes.
26267164	6	20	theme	gut	825:827	arg1	explants					829:836	chicken gut explants	817:836	chicken gut explants	817:836	An increment in bacterial adhesion to chicken gut explants was observed in L. salivarius strains with the reduction in EPS production levels.
26267164	2	21	theme	strain	398:403	arg1	E4					405:406	strain E4	398:406	strain E4	398:406	Detection of genes responsible for exopolysaccharide (EPS) production revealed that all strains harbored a dextransucrase gene, but p-gtf gene was only detected in strain E4.
26267164	6	22	from	reduction	885:893	arg1	levels					913:918	EPS production levels	898:918	EPS production levels	898:918	An increment in bacterial adhesion to chicken gut explants was observed in L. salivarius strains with the reduction in EPS production levels.
26267164	6	23	theme	production	902:911	arg1	levels					913:918	EPS production levels	898:918	EPS production levels	898:918	An increment in bacterial adhesion to chicken gut explants was observed in L. salivarius strains with the reduction in EPS production levels.
26267164	6	24	theme	chicken	817:823	arg1	explants					829:836	chicken gut explants	817:836	chicken gut explants	817:836	An increment in bacterial adhesion to chicken gut explants was observed in L. salivarius strains with the reduction in EPS production levels.
26267164	1	25	theme	strains	197:203	arg1	properties					158:167	functional properties	147:167	functional properties of Lactobacillus salivarius strains isolated from chicken feces	147:231	The aim of this work was to characterize functional properties of Lactobacillus salivarius strains isolated from chicken feces.
26267164	7	26	theme	strains	1124:1130	arg1	selection					1105:1113	final selection	1099:1113	final selection of these strains for technological purposes	1099:1157	This study showed that strain-specific properties can determine the functional properties of L. salivarius strains, and the interference of these properties might be crucial for final selection of these strains for technological purposes.
26267164	3	27	theme	levels	436:441	arg1	Analysis					409:416	Analysis	409:416	Analysis of EPS production levels	409:441	Analysis of EPS production levels showed significant alterations among strains tested.
26267164	0	28	theme	production	28:37	arg1	Impact					0:5	Impact	0:5	Impact of exopolysaccharide production on functional properties of some Lactobacillus salivarius strains.	0:104	Impact of exopolysaccharide production on functional properties of some Lactobacillus salivarius strains.
26267164	0	29	theme	exopolysaccharide	10:26	arg1	production					28:37	exopolysaccharide production	10:37	exopolysaccharide production	10:37	Impact of exopolysaccharide production on functional properties of some Lactobacillus salivarius strains.
26267164	4	30	theme	negative	576:583	arg1	correlation					585:595	a negative correlation	574:595	a negative correlation with biofilm formation and EPS production	574:637	Biofilm formation was found to be medium composition dependant, and there was a negative correlation with biofilm formation and EPS production.
26267164	5	31	from	level	772:776	arg1	specific					753:760	specific	753:760	specific	753:760	Autoaggregation properties and coaggregation of L. salivarius strains with chicken pathogens were appeared to be specific at strain level.
26267164	1	32	theme	chicken	219:225	arg1	feces					227:231	chicken feces	219:231	chicken feces	219:231	The aim of this work was to characterize functional properties of Lactobacillus salivarius strains isolated from chicken feces.
26267164	0	33	theme	functional	42:51	arg1	properties					53:62	functional properties	42:62	functional properties of some Lactobacillus salivarius strains	42:103	Impact of exopolysaccharide production on functional properties of some Lactobacillus salivarius strains.
26267164	5	34	with	coaggregation	671:683	arg1	pathogens					723:731	chicken pathogens	715:731	chicken pathogens	715:731	Autoaggregation properties and coaggregation of L. salivarius strains with chicken pathogens were appeared to be specific at strain level.
26267164	2	35	theme	responsible	253:263	arg1	genes					247:251	genes	247:251	genes responsible for exopolysaccharide (EPS) production	247:302	Detection of genes responsible for exopolysaccharide (EPS) production revealed that all strains harbored a dextransucrase gene, but p-gtf gene was only detected in strain E4.
26267164	3	36	theme	EPS	421:423	arg1	levels					436:441	EPS production levels	421:441	EPS production levels	421:441	Analysis of EPS production levels showed significant alterations among strains tested.
26267164	7	37	theme	final	1099:1103	arg1	selection					1105:1113	final selection	1099:1113	final selection of these strains for technological purposes	1099:1157	This study showed that strain-specific properties can determine the functional properties of L. salivarius strains, and the interference of these properties might be crucial for final selection of these strains for technological purposes.
26267164	1	38	attach	isolated	205:212	arg1	feces					227:231	chicken feces	219:231	chicken feces	219:231	The aim of this work was to characterize functional properties of Lactobacillus salivarius strains isolated from chicken feces.
26267164	1	38	attach	isolated	205:212	arg2	strains					197:203	Lactobacillus salivarius strains	172:203	Lactobacillus salivarius strains isolated from chicken feces	172:231	The aim of this work was to characterize functional properties of Lactobacillus salivarius strains isolated from chicken feces.
26267164	2	39	theme	genes	247:251	arg1	Detection					234:242	Detection	234:242	Detection of genes responsible for exopolysaccharide (EPS) production	234:302	Detection of genes responsible for exopolysaccharide (EPS) production revealed that all strains harbored a dextransucrase gene, but p-gtf gene was only detected in strain E4.
26267164	6	40	theme	bacterial	795:803	arg1	adhesion					805:812	bacterial adhesion	795:812	bacterial adhesion to chicken gut explants	795:836	An increment in bacterial adhesion to chicken gut explants was observed in L. salivarius strains with the reduction in EPS production levels.
26267164	6	41	with	strains	868:874	arg1	reduction					885:893	the reduction	881:893	the reduction in EPS production levels	881:918	An increment in bacterial adhesion to chicken gut explants was observed in L. salivarius strains with the reduction in EPS production levels.
26267164	4	42	theme	composition	537:547	arg1	dependant					549:557	medium composition dependant	530:557	medium composition dependant	530:557	Biofilm formation was found to be medium composition dependant, and there was a negative correlation with biofilm formation and EPS production.
26267164	6	43	from	increment	782:790	arg1	adhesion					805:812	bacterial adhesion	795:812	bacterial adhesion to chicken gut explants	795:836	An increment in bacterial adhesion to chicken gut explants was observed in L. salivarius strains with the reduction in EPS production levels.
26267164	5	44	theme	chicken	715:721	arg1	pathogens					723:731	chicken pathogens	715:731	chicken pathogens	715:731	Autoaggregation properties and coaggregation of L. salivarius strains with chicken pathogens were appeared to be specific at strain level.
26267164	1	45	theme	work	122:125	arg1	aim					110:112	The aim	106:112	The aim of this work	106:125	The aim of this work was to characterize functional properties of Lactobacillus salivarius strains isolated from chicken feces.
26267164	7	46	theme	properties	1067:1076	arg1	interference					1045:1056	the interference	1041:1056	the interference of these properties	1041:1076	This study showed that strain-specific properties can determine the functional properties of L. salivarius strains, and the interference of these properties might be crucial for final selection of these strains for technological purposes.
26267164	7	46	theme	properties	1067:1076	arg1	crucial					1087:1093	crucial	1087:1093	crucial	1087:1093	This study showed that strain-specific properties can determine the functional properties of L. salivarius strains, and the interference of these properties might be crucial for final selection of these strains for technological purposes.
26267164	7	47	theme	strain-specific	944:958	arg1	properties					960:969	strain-specific properties	944:969	strain-specific properties	944:969	This study showed that strain-specific properties can determine the functional properties of L. salivarius strains, and the interference of these properties might be crucial for final selection of these strains for technological purposes.
26267164	4	48	theme	EPS	624:626	arg1	production					628:637	EPS production	624:637	EPS production	624:637	Biofilm formation was found to be medium composition dependant, and there was a negative correlation with biofilm formation and EPS production.
26267164	2	49	located	detected	386:393	arg2	gene					372:375	p-gtf gene	366:375	p-gtf gene	366:375	Detection of genes responsible for exopolysaccharide (EPS) production revealed that all strains harbored a dextransucrase gene, but p-gtf gene was only detected in strain E4.
26267164	2	49	located	detected	386:393	arg1	E4					405:406	strain E4	398:406	strain E4	398:406	Detection of genes responsible for exopolysaccharide (EPS) production revealed that all strains harbored a dextransucrase gene, but p-gtf gene was only detected in strain E4.
26267164	2	50	theme	dextransucrase	341:354	arg1	gene					356:359	a dextransucrase gene	339:359	a dextransucrase gene	339:359	Detection of genes responsible for exopolysaccharide (EPS) production revealed that all strains harbored a dextransucrase gene, but p-gtf gene was only detected in strain E4.
26267164	3	51	theme	significant	450:460	arg1	alterations					462:472	significant alterations	450:472	significant alterations	450:472	Analysis of EPS production levels showed significant alterations among strains tested.
26267164	0	52	theme	Lactobacillus	72:84	arg1	strains					97:103	some Lactobacillus salivarius strains	67:103	some Lactobacillus salivarius strains	67:103	Impact of exopolysaccharide production on functional properties of some Lactobacillus salivarius strains.
26267164	6	53	theme	L.	854:855	arg1	strains					868:874	L. salivarius strains	854:874	L. salivarius strains with the reduction in EPS production levels	854:918	An increment in bacterial adhesion to chicken gut explants was observed in L. salivarius strains with the reduction in EPS production levels.
26267164	2	54	theme	exopolysaccharide	269:285	arg1	production					293:302	exopolysaccharide (EPS) production	269:302	exopolysaccharide (EPS) production	269:302	Detection of genes responsible for exopolysaccharide (EPS) production revealed that all strains harbored a dextransucrase gene, but p-gtf gene was only detected in strain E4.
26267164	6	55	theme	salivarius	857:866	arg1	strains					868:874	L. salivarius strains	854:874	L. salivarius strains with the reduction in EPS production levels	854:918	An increment in bacterial adhesion to chicken gut explants was observed in L. salivarius strains with the reduction in EPS production levels.
26267164	1	56	theme	salivarius	186:195	arg1	strains					197:203	Lactobacillus salivarius strains	172:203	Lactobacillus salivarius strains isolated from chicken feces	172:231	The aim of this work was to characterize functional properties of Lactobacillus salivarius strains isolated from chicken feces.
26267164	4	57	with	correlation	585:595	arg1	formation					610:618	biofilm formation	602:618	biofilm formation	602:618	Biofilm formation was found to be medium composition dependant, and there was a negative correlation with biofilm formation and EPS production.
26267164	4	57	with	correlation	585:595	arg1	production					628:637	EPS production	624:637	EPS production	624:637	Biofilm formation was found to be medium composition dependant, and there was a negative correlation with biofilm formation and EPS production.
26267164	7	58	theme	salivarius	1017:1026	arg1	strains					1028:1034	L. salivarius strains	1014:1034	L. salivarius strains	1014:1034	This study showed that strain-specific properties can determine the functional properties of L. salivarius strains, and the interference of these properties might be crucial for final selection of these strains for technological purposes.
26267164	1	59	theme	functional	147:156	arg1	properties					158:167	functional properties	147:167	functional properties of Lactobacillus salivarius strains isolated from chicken feces	147:231	The aim of this work was to characterize functional properties of Lactobacillus salivarius strains isolated from chicken feces.
26267164	5	60	with	properties	656:665	arg1	pathogens					723:731	chicken pathogens	715:731	chicken pathogens	715:731	Autoaggregation properties and coaggregation of L. salivarius strains with chicken pathogens were appeared to be specific at strain level.
28935538	1	0	dep	PEDOT	316:320	arg1	PSS					322:324	PSS	322:324	PSS	322:324	This study reports the fabrication of highly conducting and biocompatible bacterial cellulose (BC)-gold nanoparticles (AuNPs)-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) (BC-AuNPs-PEDOT:PSS) composites for biology-device interface applications.
28935538	4	1	theme	probe	835:839	arg1	analysis					841:848	Four-point probe analysis	824:848	Four-point probe analysis	824:848	Four-point probe analysis indicated that the BC-AuNPs and BC-AuNPs-PEDOT:PSS films had high electrical conductivity.
28935538	1	2	theme	-poly	259:263	arg1	BC-AuNPs-PEDOT					328:341	BC-AuNPs-PEDOT	328:341	BC-AuNPs-PEDOT:PSS	328:345	This study reports the fabrication of highly conducting and biocompatible bacterial cellulose (BC)-gold nanoparticles (AuNPs)-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) (BC-AuNPs-PEDOT:PSS) composites for biology-device interface applications.
28935538	1	2	theme	-poly	259:263	arg1	PEDOT					316:320	PEDOT	316:320	PEDOT	316:320	This study reports the fabrication of highly conducting and biocompatible bacterial cellulose (BC)-gold nanoparticles (AuNPs)-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) (BC-AuNPs-PEDOT:PSS) composites for biology-device interface applications.
28935538	1	2	theme	-poly	259:263	arg1	sulfonate					305:313	bacterial cellulose (BC)-gold nanoparticles (AuNPs)-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate	208:313	highly conducting and biocompatible bacterial cellulose (BC)-gold nanoparticles (AuNPs)-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) (BC-AuNPs-PEDOT:PSS) composites	172:357	This study reports the fabrication of highly conducting and biocompatible bacterial cellulose (BC)-gold nanoparticles (AuNPs)-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) (BC-AuNPs-PEDOT:PSS) composites for biology-device interface applications.
28935538	3	3	theme	infrared	664:671	arg1	FTIR					687:690	FTIR	687:690	FTIR	687:690	Structural characterization, using scanning electron microscopy (SEM), atomic force microscopy (AFM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and x-ray diffraction (XRD) analysis, confirmed the uniform nature of the synthesized BC-AuNPs and BC-AuNPs-PEDOT:PSS composites.
28935538	3	3	theme	infrared	664:671	arg1	spectroscopy					673:684	infrared spectroscopy	664:684	infrared spectroscopy (FTIR)	664:691	Structural characterization, using scanning electron microscopy (SEM), atomic force microscopy (AFM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and x-ray diffraction (XRD) analysis, confirmed the uniform nature of the synthesized BC-AuNPs and BC-AuNPs-PEDOT:PSS composites.
28935538	4	4	theme	Four-point	824:833	arg1	analysis					841:848	Four-point probe analysis	824:848	Four-point probe analysis	824:848	Four-point probe analysis indicated that the BC-AuNPs and BC-AuNPs-PEDOT:PSS films had high electrical conductivity.
28935538	1	5	theme	conducting	179:188	arg1	composites					348:357	highly conducting and biocompatible bacterial cellulose (BC)-gold nanoparticles (AuNPs)-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) (BC-AuNPs-PEDOT:PSS) composites	172:357	highly conducting and biocompatible bacterial cellulose (BC)-gold nanoparticles (AuNPs)-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) (BC-AuNPs-PEDOT:PSS) composites	172:357	This study reports the fabrication of highly conducting and biocompatible bacterial cellulose (BC)-gold nanoparticles (AuNPs)-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) (BC-AuNPs-PEDOT:PSS) composites for biology-device interface applications.
28935538	3	6	theme	BC-AuNPs-PEDOT	793:806	arg1	composites					812:821	the synthesized BC-AuNPs and BC-AuNPs-PEDOT:PSS composites	764:821	composites	812:821	Structural characterization, using scanning electron microscopy (SEM), atomic force microscopy (AFM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and x-ray diffraction (XRD) analysis, confirmed the uniform nature of the synthesized BC-AuNPs and BC-AuNPs-PEDOT:PSS composites.
28935538	0	7	theme	interface	110:118	arg1	applications					120:131	biology-device interface applications	95:131	biology-device interface applications	95:131	Nano-gold assisted highly conducting and biocompatible bacterial cellulose-PEDOT:PSS films for biology-device interface applications.
28935538	3	8	theme	atomic	575:580	arg1	microscopy					588:597	atomic force microscopy	575:597	atomic force microscopy (AFM)	575:603	Structural characterization, using scanning electron microscopy (SEM), atomic force microscopy (AFM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and x-ray diffraction (XRD) analysis, confirmed the uniform nature of the synthesized BC-AuNPs and BC-AuNPs-PEDOT:PSS composites.
28935538	3	8	theme	atomic	575:580	arg1	AFM					600:602	AFM	600:602	AFM	600:602	Structural characterization, using scanning electron microscopy (SEM), atomic force microscopy (AFM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and x-ray diffraction (XRD) analysis, confirmed the uniform nature of the synthesized BC-AuNPs and BC-AuNPs-PEDOT:PSS composites.
28935538	3	9	theme	electron	548:555	arg1	SEM					569:571	SEM	569:571	SEM	569:571	Structural characterization, using scanning electron microscopy (SEM), atomic force microscopy (AFM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and x-ray diffraction (XRD) analysis, confirmed the uniform nature of the synthesized BC-AuNPs and BC-AuNPs-PEDOT:PSS composites.
28935538	3	9	theme	electron	548:555	arg1	microscopy					557:566	scanning electron microscopy	539:566	scanning electron microscopy (SEM)	539:572	Structural characterization, using scanning electron microscopy (SEM), atomic force microscopy (AFM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and x-ray diffraction (XRD) analysis, confirmed the uniform nature of the synthesized BC-AuNPs and BC-AuNPs-PEDOT:PSS composites.
28935538	8	10	theme	candidate	1347:1355	arg1	composites					1332:1341	our BC-AuNPs-PEDOT:PSS composites	1309:1341	our BC-AuNPs-PEDOT:PSS composites	1309:1341	Therefore, our BC-AuNPs-PEDOT:PSS composites are candidate materials for biology-device interfaces to produce implantable devices in regenerative medicine.
28935538	8	10	theme	candidate	1347:1355	arg1	materials					1357:1365	candidate materials	1347:1365	candidate materials for biology-device interfaces	1347:1395	Therefore, our BC-AuNPs-PEDOT:PSS composites are candidate materials for biology-device interfaces to produce implantable devices in regenerative medicine.
28935538	1	11	theme	3,4-ethylenedioxythiophene	265:290	arg1	BC-AuNPs-PEDOT					328:341	BC-AuNPs-PEDOT	328:341	BC-AuNPs-PEDOT:PSS	328:345	This study reports the fabrication of highly conducting and biocompatible bacterial cellulose (BC)-gold nanoparticles (AuNPs)-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) (BC-AuNPs-PEDOT:PSS) composites for biology-device interface applications.
28935538	1	11	theme	3,4-ethylenedioxythiophene	265:290	arg1	PEDOT					316:320	PEDOT	316:320	PEDOT	316:320	This study reports the fabrication of highly conducting and biocompatible bacterial cellulose (BC)-gold nanoparticles (AuNPs)-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) (BC-AuNPs-PEDOT:PSS) composites for biology-device interface applications.
28935538	1	11	theme	3,4-ethylenedioxythiophene	265:290	arg1	sulfonate					305:313	bacterial cellulose (BC)-gold nanoparticles (AuNPs)-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate	208:313	highly conducting and biocompatible bacterial cellulose (BC)-gold nanoparticles (AuNPs)-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) (BC-AuNPs-PEDOT:PSS) composites	172:357	This study reports the fabrication of highly conducting and biocompatible bacterial cellulose (BC)-gold nanoparticles (AuNPs)-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) (BC-AuNPs-PEDOT:PSS) composites for biology-device interface applications.
28935538	0	12	theme	biology-device	95:108	arg1	applications					120:131	biology-device interface applications	95:131	biology-device interface applications	95:131	Nano-gold assisted highly conducting and biocompatible bacterial cellulose-PEDOT:PSS films for biology-device interface applications.
28935538	1	13	theme	composites	348:357	arg1	fabrication					157:167	the fabrication	153:167	the fabrication of highly conducting and biocompatible bacterial cellulose (BC)-gold nanoparticles (AuNPs)-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) (BC-AuNPs-PEDOT:PSS) composites for biology-device interface applications	153:399	This study reports the fabrication of highly conducting and biocompatible bacterial cellulose (BC)-gold nanoparticles (AuNPs)-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) (BC-AuNPs-PEDOT:PSS) composites for biology-device interface applications.
28935538	7	14	theme	voltage	1205:1211	arg1	response					1213:1220	a voltage response	1203:1220	a voltage response	1203:1220	AuNPs and PEDOT:PSS, imparted a voltage response, while BC imparted biocompatibility and bio-adhesion to the nanocomposites.
28935538	3	15	theme	BC-AuNPs	780:787	arg1	nature					754:759	the uniform nature	742:759	the uniform nature of the synthesized BC-AuNPs and BC-AuNPs-PEDOT:PSS composites	742:821	Structural characterization, using scanning electron microscopy (SEM), atomic force microscopy (AFM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and x-ray diffraction (XRD) analysis, confirmed the uniform nature of the synthesized BC-AuNPs and BC-AuNPs-PEDOT:PSS composites.
28935538	3	16	theme	x-ray	698:702	arg1	XRD					717:719	XRD	717:719	XRD	717:719	Structural characterization, using scanning electron microscopy (SEM), atomic force microscopy (AFM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and x-ray diffraction (XRD) analysis, confirmed the uniform nature of the synthesized BC-AuNPs and BC-AuNPs-PEDOT:PSS composites.
28935538	3	16	theme	x-ray	698:702	arg1	diffraction					704:714	x-ray diffraction	698:714	x-ray diffraction (XRD)	698:720	Structural characterization, using scanning electron microscopy (SEM), atomic force microscopy (AFM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and x-ray diffraction (XRD) analysis, confirmed the uniform nature of the synthesized BC-AuNPs and BC-AuNPs-PEDOT:PSS composites.
28935538	3	17	dep	Fourier	646:652	arg1	transform					654:662	transform	654:662	transform infrared spectroscopy (FTIR)	654:691	Structural characterization, using scanning electron microscopy (SEM), atomic force microscopy (AFM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and x-ray diffraction (XRD) analysis, confirmed the uniform nature of the synthesized BC-AuNPs and BC-AuNPs-PEDOT:PSS composites.
28935538	3	18	theme	synthesized	768:778	arg1	BC-AuNPs					780:787	the synthesized BC-AuNPs and BC-AuNPs-PEDOT:PSS composites	764:821	BC-AuNPs	780:787	Structural characterization, using scanning electron microscopy (SEM), atomic force microscopy (AFM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and x-ray diffraction (XRD) analysis, confirmed the uniform nature of the synthesized BC-AuNPs and BC-AuNPs-PEDOT:PSS composites.
28935538	4	19	theme	electrical	916:925	arg1	conductivity					927:938	high electrical conductivity	911:938	high electrical conductivity	911:938	Four-point probe analysis indicated that the BC-AuNPs and BC-AuNPs-PEDOT:PSS films had high electrical conductivity.
28935538	1	20	theme	biology-device	363:376	arg1	applications					388:399	biology-device interface applications	363:399	biology-device interface applications	363:399	This study reports the fabrication of highly conducting and biocompatible bacterial cellulose (BC)-gold nanoparticles (AuNPs)-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) (BC-AuNPs-PEDOT:PSS) composites for biology-device interface applications.
28935538	3	21	theme	scanning	539:546	arg1	SEM					569:571	SEM	569:571	SEM	569:571	Structural characterization, using scanning electron microscopy (SEM), atomic force microscopy (AFM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and x-ray diffraction (XRD) analysis, confirmed the uniform nature of the synthesized BC-AuNPs and BC-AuNPs-PEDOT:PSS composites.
28935538	3	21	theme	scanning	539:546	arg1	microscopy					557:566	scanning electron microscopy	539:566	scanning electron microscopy (SEM)	539:572	Structural characterization, using scanning electron microscopy (SEM), atomic force microscopy (AFM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and x-ray diffraction (XRD) analysis, confirmed the uniform nature of the synthesized BC-AuNPs and BC-AuNPs-PEDOT:PSS composites.
28935538	1	22	theme	biocompatible	194:206	arg1	composites					348:357	highly conducting and biocompatible bacterial cellulose (BC)-gold nanoparticles (AuNPs)-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) (BC-AuNPs-PEDOT:PSS) composites	172:357	highly conducting and biocompatible bacterial cellulose (BC)-gold nanoparticles (AuNPs)-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) (BC-AuNPs-PEDOT:PSS) composites	172:357	This study reports the fabrication of highly conducting and biocompatible bacterial cellulose (BC)-gold nanoparticles (AuNPs)-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) (BC-AuNPs-PEDOT:PSS) composites for biology-device interface applications.
28935538	0	23	theme	assisted	10:17	arg1	cellulose-PEDOT					65:79	Nano-gold assisted highly conducting and biocompatible bacterial cellulose-PEDOT	0:79	Nano-gold assisted highly conducting and biocompatible bacterial cellulose-PEDOT:PSS films for biology-device interface applications.	0:132	Nano-gold assisted highly conducting and biocompatible bacterial cellulose-PEDOT:PSS films for biology-device interface applications.
28935538	1	24	theme	interface	378:386	arg1	applications					388:399	biology-device interface applications	363:399	biology-device interface applications	363:399	This study reports the fabrication of highly conducting and biocompatible bacterial cellulose (BC)-gold nanoparticles (AuNPs)-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) (BC-AuNPs-PEDOT:PSS) composites for biology-device interface applications.
28935538	2	25	dep	AuNPs	464:468	arg1	PSS					480:482	PSS	480:482	AuNPs and PEDOT:PSS	464:482	The composites were fabricated using ex situ incorporation of AuNPs and PEDOT:PSS into the BC matrix.
28935538	1	26	theme	bacterial	208:216	arg1	BC-AuNPs-PEDOT					328:341	BC-AuNPs-PEDOT	328:341	BC-AuNPs-PEDOT:PSS	328:345	This study reports the fabrication of highly conducting and biocompatible bacterial cellulose (BC)-gold nanoparticles (AuNPs)-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) (BC-AuNPs-PEDOT:PSS) composites for biology-device interface applications.
28935538	1	26	theme	bacterial	208:216	arg1	PEDOT					316:320	PEDOT	316:320	PEDOT	316:320	This study reports the fabrication of highly conducting and biocompatible bacterial cellulose (BC)-gold nanoparticles (AuNPs)-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) (BC-AuNPs-PEDOT:PSS) composites for biology-device interface applications.
28935538	1	26	theme	bacterial	208:216	arg1	sulfonate					305:313	bacterial cellulose (BC)-gold nanoparticles (AuNPs)-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate	208:313	highly conducting and biocompatible bacterial cellulose (BC)-gold nanoparticles (AuNPs)-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) (BC-AuNPs-PEDOT:PSS) composites	172:357	This study reports the fabrication of highly conducting and biocompatible bacterial cellulose (BC)-gold nanoparticles (AuNPs)-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) (BC-AuNPs-PEDOT:PSS) composites for biology-device interface applications.
28935538	0	27	theme	Nano-gold	0:8	arg1	cellulose-PEDOT					65:79	Nano-gold assisted highly conducting and biocompatible bacterial cellulose-PEDOT	0:79	Nano-gold assisted highly conducting and biocompatible bacterial cellulose-PEDOT:PSS films for biology-device interface applications.	0:132	Nano-gold assisted highly conducting and biocompatible bacterial cellulose-PEDOT:PSS films for biology-device interface applications.
28935538	1	28	theme	polystyrene	293:303	arg1	BC-AuNPs-PEDOT					328:341	BC-AuNPs-PEDOT	328:341	BC-AuNPs-PEDOT:PSS	328:345	This study reports the fabrication of highly conducting and biocompatible bacterial cellulose (BC)-gold nanoparticles (AuNPs)-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) (BC-AuNPs-PEDOT:PSS) composites for biology-device interface applications.
28935538	1	28	theme	polystyrene	293:303	arg1	PEDOT					316:320	PEDOT	316:320	PEDOT	316:320	This study reports the fabrication of highly conducting and biocompatible bacterial cellulose (BC)-gold nanoparticles (AuNPs)-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) (BC-AuNPs-PEDOT:PSS) composites for biology-device interface applications.
28935538	1	28	theme	polystyrene	293:303	arg1	sulfonate					305:313	bacterial cellulose (BC)-gold nanoparticles (AuNPs)-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate	208:313	highly conducting and biocompatible bacterial cellulose (BC)-gold nanoparticles (AuNPs)-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) (BC-AuNPs-PEDOT:PSS) composites	172:357	This study reports the fabrication of highly conducting and biocompatible bacterial cellulose (BC)-gold nanoparticles (AuNPs)-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) (BC-AuNPs-PEDOT:PSS) composites for biology-device interface applications.
28935538	1	29	theme	cellulose	218:226	arg1	BC-AuNPs-PEDOT					328:341	BC-AuNPs-PEDOT	328:341	BC-AuNPs-PEDOT:PSS	328:345	This study reports the fabrication of highly conducting and biocompatible bacterial cellulose (BC)-gold nanoparticles (AuNPs)-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) (BC-AuNPs-PEDOT:PSS) composites for biology-device interface applications.
28935538	1	29	theme	cellulose	218:226	arg1	PEDOT					316:320	PEDOT	316:320	PEDOT	316:320	This study reports the fabrication of highly conducting and biocompatible bacterial cellulose (BC)-gold nanoparticles (AuNPs)-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) (BC-AuNPs-PEDOT:PSS) composites for biology-device interface applications.
28935538	1	29	theme	cellulose	218:226	arg1	sulfonate					305:313	bacterial cellulose (BC)-gold nanoparticles (AuNPs)-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate	208:313	highly conducting and biocompatible bacterial cellulose (BC)-gold nanoparticles (AuNPs)-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) (BC-AuNPs-PEDOT:PSS) composites	172:357	This study reports the fabrication of highly conducting and biocompatible bacterial cellulose (BC)-gold nanoparticles (AuNPs)-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) (BC-AuNPs-PEDOT:PSS) composites for biology-device interface applications.
28935538	0	30	theme	conducting	26:35	arg1	cellulose-PEDOT					65:79	Nano-gold assisted highly conducting and biocompatible bacterial cellulose-PEDOT	0:79	Nano-gold assisted highly conducting and biocompatible bacterial cellulose-PEDOT:PSS films for biology-device interface applications.	0:132	Nano-gold assisted highly conducting and biocompatible bacterial cellulose-PEDOT:PSS films for biology-device interface applications.
28935538	1	31	theme	sulfonate	305:313	arg1	composites					348:357	highly conducting and biocompatible bacterial cellulose (BC)-gold nanoparticles (AuNPs)-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) (BC-AuNPs-PEDOT:PSS) composites	172:357	highly conducting and biocompatible bacterial cellulose (BC)-gold nanoparticles (AuNPs)-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) (BC-AuNPs-PEDOT:PSS) composites	172:357	This study reports the fabrication of highly conducting and biocompatible bacterial cellulose (BC)-gold nanoparticles (AuNPs)-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) (BC-AuNPs-PEDOT:PSS) composites for biology-device interface applications.
28935538	8	32	theme	biology-device	1371:1384	arg1	interfaces					1386:1395	biology-device interfaces	1371:1395	biology-device interfaces	1371:1395	Therefore, our BC-AuNPs-PEDOT:PSS composites are candidate materials for biology-device interfaces to produce implantable devices in regenerative medicine.
28935538	2	33	theme	ex	439:440	arg1	incorporation					447:459	ex situ incorporation	439:459	ex situ incorporation of AuNPs and PEDOT:PSS into the BC matrix	439:501	The composites were fabricated using ex situ incorporation of AuNPs and PEDOT:PSS into the BC matrix.
28935538	1	34	dep	BC-AuNPs-PEDOT	328:341	arg1	PSS					343:345	PSS	343:345	BC-AuNPs-PEDOT:PSS	328:345	This study reports the fabrication of highly conducting and biocompatible bacterial cellulose (BC)-gold nanoparticles (AuNPs)-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) (BC-AuNPs-PEDOT:PSS) composites for biology-device interface applications.
28935538	4	35	theme	BC-AuNPs-PEDOT	882:895	arg1	films					901:905	the BC-AuNPs and BC-AuNPs-PEDOT:PSS films	865:905	the BC-AuNPs and BC-AuNPs-PEDOT:PSS films	865:905	Four-point probe analysis indicated that the BC-AuNPs and BC-AuNPs-PEDOT:PSS films had high electrical conductivity.
28935538	3	36	theme	Structural	504:513	arg1	characterization					515:530	Structural characterization	504:530	Structural characterization	504:530	Structural characterization, using scanning electron microscopy (SEM), atomic force microscopy (AFM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and x-ray diffraction (XRD) analysis, confirmed the uniform nature of the synthesized BC-AuNPs and BC-AuNPs-PEDOT:PSS composites.
28935538	1	37	theme	BC	229:230	arg1	BC-AuNPs-PEDOT					328:341	BC-AuNPs-PEDOT	328:341	BC-AuNPs-PEDOT:PSS	328:345	This study reports the fabrication of highly conducting and biocompatible bacterial cellulose (BC)-gold nanoparticles (AuNPs)-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) (BC-AuNPs-PEDOT:PSS) composites for biology-device interface applications.
28935538	1	37	theme	BC	229:230	arg1	PEDOT					316:320	PEDOT	316:320	PEDOT	316:320	This study reports the fabrication of highly conducting and biocompatible bacterial cellulose (BC)-gold nanoparticles (AuNPs)-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) (BC-AuNPs-PEDOT:PSS) composites for biology-device interface applications.
28935538	1	37	theme	BC	229:230	arg1	sulfonate					305:313	bacterial cellulose (BC)-gold nanoparticles (AuNPs)-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate	208:313	highly conducting and biocompatible bacterial cellulose (BC)-gold nanoparticles (AuNPs)-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) (BC-AuNPs-PEDOT:PSS) composites	172:357	This study reports the fabrication of highly conducting and biocompatible bacterial cellulose (BC)-gold nanoparticles (AuNPs)-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) (BC-AuNPs-PEDOT:PSS) composites for biology-device interface applications.
28935538	0	38	theme	biocompatible	41:53	arg1	cellulose-PEDOT					65:79	Nano-gold assisted highly conducting and biocompatible bacterial cellulose-PEDOT	0:79	Nano-gold assisted highly conducting and biocompatible bacterial cellulose-PEDOT:PSS films for biology-device interface applications.	0:132	Nano-gold assisted highly conducting and biocompatible bacterial cellulose-PEDOT:PSS films for biology-device interface applications.
28935538	8	39	theme	regenerative	1431:1442	arg1	medicine					1444:1451	regenerative medicine	1431:1451	regenerative medicine	1431:1451	Therefore, our BC-AuNPs-PEDOT:PSS composites are candidate materials for biology-device interfaces to produce implantable devices in regenerative medicine.
28935538	0	40	dep	cellulose-PEDOT	65:79	arg1	films					85:89	PSS films	81:89	Nano-gold assisted highly conducting and biocompatible bacterial cellulose-PEDOT:PSS films for biology-device interface applications.	0:132	Nano-gold assisted highly conducting and biocompatible bacterial cellulose-PEDOT:PSS films for biology-device interface applications.
28935538	6	41	theme	composite	1034:1042	arg1	films					1044:1048	The composite films	1030:1048	The composite films	1030:1048	The composite films supported adhesion, growth, and proliferation of MC3T3-E1 cells, indicating that they are biocompatible and non-cytotoxic.
28935538	8	42	dep	BC-AuNPs-PEDOT	1313:1326	arg1	PSS					1328:1330	PSS	1328:1330	PSS	1328:1330	Therefore, our BC-AuNPs-PEDOT:PSS composites are candidate materials for biology-device interfaces to produce implantable devices in regenerative medicine.
28935538	4	43	theme	PSS	897:899	arg1	films					901:905	the BC-AuNPs and BC-AuNPs-PEDOT:PSS films	865:905	the BC-AuNPs and BC-AuNPs-PEDOT:PSS films	865:905	Four-point probe analysis indicated that the BC-AuNPs and BC-AuNPs-PEDOT:PSS films had high electrical conductivity.
28935538	3	44	theme	electron	619:626	arg1	TEM					640:642	TEM	640:642	TEM	640:642	Structural characterization, using scanning electron microscopy (SEM), atomic force microscopy (AFM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and x-ray diffraction (XRD) analysis, confirmed the uniform nature of the synthesized BC-AuNPs and BC-AuNPs-PEDOT:PSS composites.
28935538	3	44	theme	electron	619:626	arg1	microscopy					628:637	transmission electron microscopy	606:637	transmission electron microscopy (TEM)	606:643	Structural characterization, using scanning electron microscopy (SEM), atomic force microscopy (AFM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and x-ray diffraction (XRD) analysis, confirmed the uniform nature of the synthesized BC-AuNPs and BC-AuNPs-PEDOT:PSS composites.
28935538	3	45	theme	uniform	746:752	arg1	nature					754:759	the uniform nature	742:759	the uniform nature of the synthesized BC-AuNPs and BC-AuNPs-PEDOT:PSS composites	742:821	Structural characterization, using scanning electron microscopy (SEM), atomic force microscopy (AFM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and x-ray diffraction (XRD) analysis, confirmed the uniform nature of the synthesized BC-AuNPs and BC-AuNPs-PEDOT:PSS composites.
28935538	1	46	theme	-gold	232:236	arg1	BC-AuNPs-PEDOT					328:341	BC-AuNPs-PEDOT	328:341	BC-AuNPs-PEDOT:PSS	328:345	This study reports the fabrication of highly conducting and biocompatible bacterial cellulose (BC)-gold nanoparticles (AuNPs)-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) (BC-AuNPs-PEDOT:PSS) composites for biology-device interface applications.
28935538	1	46	theme	-gold	232:236	arg1	PEDOT					316:320	PEDOT	316:320	PEDOT	316:320	This study reports the fabrication of highly conducting and biocompatible bacterial cellulose (BC)-gold nanoparticles (AuNPs)-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) (BC-AuNPs-PEDOT:PSS) composites for biology-device interface applications.
28935538	1	46	theme	-gold	232:236	arg1	sulfonate					305:313	bacterial cellulose (BC)-gold nanoparticles (AuNPs)-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate	208:313	highly conducting and biocompatible bacterial cellulose (BC)-gold nanoparticles (AuNPs)-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) (BC-AuNPs-PEDOT:PSS) composites	172:357	This study reports the fabrication of highly conducting and biocompatible bacterial cellulose (BC)-gold nanoparticles (AuNPs)-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) (BC-AuNPs-PEDOT:PSS) composites for biology-device interface applications.
28935538	2	47	theme	PEDOT	474:478	arg1	incorporation					447:459	ex situ incorporation	439:459	ex situ incorporation of AuNPs and PEDOT:PSS into the BC matrix	439:501	The composites were fabricated using ex situ incorporation of AuNPs and PEDOT:PSS into the BC matrix.
28935538	4	48	theme	high	911:914	arg1	conductivity					927:938	high electrical conductivity	911:938	high electrical conductivity	911:938	Four-point probe analysis indicated that the BC-AuNPs and BC-AuNPs-PEDOT:PSS films had high electrical conductivity.
28935538	8	49	theme	BC-AuNPs-PEDOT	1313:1326	arg1	composites					1332:1341	our BC-AuNPs-PEDOT:PSS composites	1309:1341	our BC-AuNPs-PEDOT:PSS composites	1309:1341	Therefore, our BC-AuNPs-PEDOT:PSS composites are candidate materials for biology-device interfaces to produce implantable devices in regenerative medicine.
28935538	8	49	theme	BC-AuNPs-PEDOT	1313:1326	arg1	materials					1357:1365	candidate materials	1347:1365	candidate materials for biology-device interfaces	1347:1395	Therefore, our BC-AuNPs-PEDOT:PSS composites are candidate materials for biology-device interfaces to produce implantable devices in regenerative medicine.
28935538	5	50	with	biocompatibility	977:992	arg1	MC3T3-E1					1019:1026	MC3T3-E1	1019:1026	MC3T3-E1	1019:1026	The composites were also tested for biocompatibility with animal osteoblasts (MC3T3-E1).
28935538	5	50	with	biocompatibility	977:992	arg1	osteoblasts					1006:1016	animal osteoblasts	999:1016	animal osteoblasts (MC3T3-E1)	999:1027	The composites were also tested for biocompatibility with animal osteoblasts (MC3T3-E1).
28935538	2	51	dep	ex	439:440	arg1	situ					442:445	situ	442:445	situ	442:445	The composites were fabricated using ex situ incorporation of AuNPs and PEDOT:PSS into the BC matrix.
28935538	6	52	theme	cells	1108:1112	arg1	growth					1070:1075	growth	1070:1075	growth	1070:1075	The composite films supported adhesion, growth, and proliferation of MC3T3-E1 cells, indicating that they are biocompatible and non-cytotoxic.
28935538	6	52	theme	cells	1108:1112	arg1	proliferation					1082:1094	proliferation	1082:1094	proliferation	1082:1094	The composite films supported adhesion, growth, and proliferation of MC3T3-E1 cells, indicating that they are biocompatible and non-cytotoxic.
28935538	6	52	theme	cells	1108:1112	arg1	adhesion					1060:1067	adhesion	1060:1067	adhesion	1060:1067	The composite films supported adhesion, growth, and proliferation of MC3T3-E1 cells, indicating that they are biocompatible and non-cytotoxic.
28935538	0	53	theme	bacterial	55:63	arg1	cellulose-PEDOT					65:79	Nano-gold assisted highly conducting and biocompatible bacterial cellulose-PEDOT	0:79	Nano-gold assisted highly conducting and biocompatible bacterial cellulose-PEDOT:PSS films for biology-device interface applications.	0:132	Nano-gold assisted highly conducting and biocompatible bacterial cellulose-PEDOT:PSS films for biology-device interface applications.
28935538	7	54	dep	AuNPs	1173:1177	arg1	imparted					1194:1201	imparted	1194:1201	imparted	1194:1201	AuNPs and PEDOT:PSS, imparted a voltage response, while BC imparted biocompatibility and bio-adhesion to the nanocomposites.
28935538	3	55	theme	force	582:586	arg1	microscopy					588:597	atomic force microscopy	575:597	atomic force microscopy (AFM)	575:603	Structural characterization, using scanning electron microscopy (SEM), atomic force microscopy (AFM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and x-ray diffraction (XRD) analysis, confirmed the uniform nature of the synthesized BC-AuNPs and BC-AuNPs-PEDOT:PSS composites.
28935538	3	55	theme	force	582:586	arg1	AFM					600:602	AFM	600:602	AFM	600:602	Structural characterization, using scanning electron microscopy (SEM), atomic force microscopy (AFM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and x-ray diffraction (XRD) analysis, confirmed the uniform nature of the synthesized BC-AuNPs and BC-AuNPs-PEDOT:PSS composites.
28935538	4	56	contain	had	907:909	arg2	conductivity					927:938	high electrical conductivity	911:938	high electrical conductivity	911:938	Four-point probe analysis indicated that the BC-AuNPs and BC-AuNPs-PEDOT:PSS films had high electrical conductivity.
28935538	4	56	contain	had	907:909	arg1	films					901:905	the BC-AuNPs and BC-AuNPs-PEDOT:PSS films	865:905	the BC-AuNPs and BC-AuNPs-PEDOT:PSS films	865:905	Four-point probe analysis indicated that the BC-AuNPs and BC-AuNPs-PEDOT:PSS films had high electrical conductivity.
28935538	6	57	theme	MC3T3-E1	1099:1106	arg1	cells					1108:1112	MC3T3-E1 cells	1099:1112	MC3T3-E1 cells	1099:1112	The composite films supported adhesion, growth, and proliferation of MC3T3-E1 cells, indicating that they are biocompatible and non-cytotoxic.
28935538	3	58	theme	composites	812:821	arg1	nature					754:759	the uniform nature	742:759	the uniform nature of the synthesized BC-AuNPs and BC-AuNPs-PEDOT:PSS composites	742:821	Structural characterization, using scanning electron microscopy (SEM), atomic force microscopy (AFM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and x-ray diffraction (XRD) analysis, confirmed the uniform nature of the synthesized BC-AuNPs and BC-AuNPs-PEDOT:PSS composites.
28935538	8	59	from	devices	1420:1426	arg1	medicine					1444:1451	regenerative medicine	1431:1451	regenerative medicine	1431:1451	Therefore, our BC-AuNPs-PEDOT:PSS composites are candidate materials for biology-device interfaces to produce implantable devices in regenerative medicine.
28935538	8	60	theme	implantable	1408:1418	arg1	devices					1420:1426	implantable devices	1408:1426	implantable devices in regenerative medicine	1408:1451	Therefore, our BC-AuNPs-PEDOT:PSS composites are candidate materials for biology-device interfaces to produce implantable devices in regenerative medicine.
28935538	5	61	theme	animal	999:1004	arg1	MC3T3-E1					1019:1026	MC3T3-E1	1019:1026	MC3T3-E1	1019:1026	The composites were also tested for biocompatibility with animal osteoblasts (MC3T3-E1).
28935538	5	61	theme	animal	999:1004	arg1	osteoblasts					1006:1016	animal osteoblasts	999:1016	animal osteoblasts (MC3T3-E1)	999:1027	The composites were also tested for biocompatibility with animal osteoblasts (MC3T3-E1).
28935538	1	62	theme	nanoparticles	238:250	arg1	BC-AuNPs-PEDOT					328:341	BC-AuNPs-PEDOT	328:341	BC-AuNPs-PEDOT:PSS	328:345	This study reports the fabrication of highly conducting and biocompatible bacterial cellulose (BC)-gold nanoparticles (AuNPs)-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) (BC-AuNPs-PEDOT:PSS) composites for biology-device interface applications.
28935538	1	62	theme	nanoparticles	238:250	arg1	PEDOT					316:320	PEDOT	316:320	PEDOT	316:320	This study reports the fabrication of highly conducting and biocompatible bacterial cellulose (BC)-gold nanoparticles (AuNPs)-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) (BC-AuNPs-PEDOT:PSS) composites for biology-device interface applications.
28935538	1	62	theme	nanoparticles	238:250	arg1	sulfonate					305:313	bacterial cellulose (BC)-gold nanoparticles (AuNPs)-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate	208:313	highly conducting and biocompatible bacterial cellulose (BC)-gold nanoparticles (AuNPs)-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) (BC-AuNPs-PEDOT:PSS) composites	172:357	This study reports the fabrication of highly conducting and biocompatible bacterial cellulose (BC)-gold nanoparticles (AuNPs)-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) (BC-AuNPs-PEDOT:PSS) composites for biology-device interface applications.
28935538	4	63	theme	BC-AuNPs	869:876	arg1	films					901:905	the BC-AuNPs and BC-AuNPs-PEDOT:PSS films	865:905	the BC-AuNPs and BC-AuNPs-PEDOT:PSS films	865:905	Four-point probe analysis indicated that the BC-AuNPs and BC-AuNPs-PEDOT:PSS films had high electrical conductivity.
28935538	3	64	theme	transmission	606:617	arg1	TEM					640:642	TEM	640:642	TEM	640:642	Structural characterization, using scanning electron microscopy (SEM), atomic force microscopy (AFM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and x-ray diffraction (XRD) analysis, confirmed the uniform nature of the synthesized BC-AuNPs and BC-AuNPs-PEDOT:PSS composites.
28935538	3	64	theme	transmission	606:617	arg1	microscopy					628:637	transmission electron microscopy	606:637	transmission electron microscopy (TEM)	606:643	Structural characterization, using scanning electron microscopy (SEM), atomic force microscopy (AFM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and x-ray diffraction (XRD) analysis, confirmed the uniform nature of the synthesized BC-AuNPs and BC-AuNPs-PEDOT:PSS composites.
28935538	3	65	dep	microscopy	557:566	arg1	analysis					722:729	analysis	722:729	analysis	722:729	Structural characterization, using scanning electron microscopy (SEM), atomic force microscopy (AFM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and x-ray diffraction (XRD) analysis, confirmed the uniform nature of the synthesized BC-AuNPs and BC-AuNPs-PEDOT:PSS composites.
28935538	0	66	theme	PSS	81:83	arg1	films					85:89	PSS films	81:89	Nano-gold assisted highly conducting and biocompatible bacterial cellulose-PEDOT:PSS films for biology-device interface applications.	0:132	Nano-gold assisted highly conducting and biocompatible bacterial cellulose-PEDOT:PSS films for biology-device interface applications.
28935538	3	67	theme	PSS	808:810	arg1	composites					812:821	the synthesized BC-AuNPs and BC-AuNPs-PEDOT:PSS composites	764:821	composites	812:821	Structural characterization, using scanning electron microscopy (SEM), atomic force microscopy (AFM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and x-ray diffraction (XRD) analysis, confirmed the uniform nature of the synthesized BC-AuNPs and BC-AuNPs-PEDOT:PSS composites.
28935538	1	68	theme	AuNPs	253:257	arg1	BC-AuNPs-PEDOT					328:341	BC-AuNPs-PEDOT	328:341	BC-AuNPs-PEDOT:PSS	328:345	This study reports the fabrication of highly conducting and biocompatible bacterial cellulose (BC)-gold nanoparticles (AuNPs)-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) (BC-AuNPs-PEDOT:PSS) composites for biology-device interface applications.
28935538	1	68	theme	AuNPs	253:257	arg1	PEDOT					316:320	PEDOT	316:320	PEDOT	316:320	This study reports the fabrication of highly conducting and biocompatible bacterial cellulose (BC)-gold nanoparticles (AuNPs)-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) (BC-AuNPs-PEDOT:PSS) composites for biology-device interface applications.
28935538	1	68	theme	AuNPs	253:257	arg1	sulfonate					305:313	bacterial cellulose (BC)-gold nanoparticles (AuNPs)-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate	208:313	highly conducting and biocompatible bacterial cellulose (BC)-gold nanoparticles (AuNPs)-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) (BC-AuNPs-PEDOT:PSS) composites	172:357	This study reports the fabrication of highly conducting and biocompatible bacterial cellulose (BC)-gold nanoparticles (AuNPs)-poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) (BC-AuNPs-PEDOT:PSS) composites for biology-device interface applications.
28935538	2	69	theme	BC	493:494	arg1	matrix					496:501	the BC matrix	489:501	the BC matrix	489:501	The composites were fabricated using ex situ incorporation of AuNPs and PEDOT:PSS into the BC matrix.
28935538	2	70	theme	AuNPs	464:468	arg1	incorporation					447:459	ex situ incorporation	439:459	ex situ incorporation of AuNPs and PEDOT:PSS into the BC matrix	439:501	The composites were fabricated using ex situ incorporation of AuNPs and PEDOT:PSS into the BC matrix.
24675830	0	0	theme	rabbit	71:76	arg1	model					95:99	a rabbit segmental defect model	69:99	a rabbit segmental defect model	69:99	Comparable bone healing capacity of different bone graft matrices in a rabbit segmental defect model.
24675830	5	1	with	products	1125:1132	arg1	compositions					1175:1186	bone compositions	1170:1186	bone compositions	1170:1186	Overall, three DBM products with different carrier molecules or bone compositions showed similar bone healing capacity.
24675830	5	1	with	products	1125:1132	arg1	molecules					1157:1165	different carrier molecules	1139:1165	different carrier molecules	1139:1165	Overall, three DBM products with different carrier molecules or bone compositions showed similar bone healing capacity.
24675830	2	2	theme	cortical	557:564	arg1	matrix					571:576	HA-based demineralized cortical bone matrix	534:576	HA-based demineralized cortical bone matrix (DBX)	534:582	Overall, 15-mm segmental defects in the left and right radiuses were created in 36 New Zealand White rabbits and filled with HA-based demineralized cortical bone matrix (DBX), CMC-based demineralized cortical bone matrix (DB) or CMC-based demineralized cortical bone with cancellous bone (NDDB), and the wound area was evaluated at 4, 8, and 12 weeks post-implantation.
24675830	2	2	theme	cortical	557:564	arg1	DBX					579:581	DBX	579:581	DBX	579:581	Overall, 15-mm segmental defects in the left and right radiuses were created in 36 New Zealand White rabbits and filled with HA-based demineralized cortical bone matrix (DBX), CMC-based demineralized cortical bone matrix (DB) or CMC-based demineralized cortical bone with cancellous bone (NDDB), and the wound area was evaluated at 4, 8, and 12 weeks post-implantation.
24675830	2	3	theme	demineralized	648:660	arg1	bone					671:674	CMC-based demineralized cortical bone	638:674	CMC-based demineralized cortical bone with cancellous bone (NDDB)	638:702	Overall, 15-mm segmental defects in the left and right radiuses were created in 36 New Zealand White rabbits and filled with HA-based demineralized cortical bone matrix (DBX), CMC-based demineralized cortical bone matrix (DB) or CMC-based demineralized cortical bone with cancellous bone (NDDB), and the wound area was evaluated at 4, 8, and 12 weeks post-implantation.
24675830	3	4	theme	lower	804:808	arg1	radiopacity					810:820	significantly lower radiopacity	790:820	significantly lower radiopacity	790:820	DBX showed significantly lower radiopacity, bone volume fraction, and bone mineral density than DB and NDDB before implantation.
24675830	1	5	dep	molecules	232:240	arg1	[HA					259:261	hyaluronic acid [HA] vs. carboxymethylcellulose [CMC	243:294	[HA	259:261	We compared the bone healing capacity of three different demineralized bone matrix (DBM) products applied using different carrier molecules (hyaluronic acid [HA] vs. carboxymethylcellulose [CMC]) or bone compositions (cortical bone vs. cortical bone and cancellous bone) in a rabbit segmental defect model.
24675830	1	5	dep	molecules	232:240	arg1	[CMC					291:294	hyaluronic acid [HA] vs. carboxymethylcellulose [CMC	243:294	[CMC	291:294	We compared the bone healing capacity of three different demineralized bone matrix (DBM) products applied using different carrier molecules (hyaluronic acid [HA] vs. carboxymethylcellulose [CMC]) or bone compositions (cortical bone vs. cortical bone and cancellous bone) in a rabbit segmental defect model.
24675830	1	6	theme	defect	395:400	arg1	model					402:406	a rabbit segmental defect model	376:406	a rabbit segmental defect model	376:406	We compared the bone healing capacity of three different demineralized bone matrix (DBM) products applied using different carrier molecules (hyaluronic acid [HA] vs. carboxymethylcellulose [CMC]) or bone compositions (cortical bone vs. cortical bone and cancellous bone) in a rabbit segmental defect model.
24675830	5	7	theme	DBM	1121:1123	arg1	products					1125:1132	three DBM products	1115:1132	three DBM products with different carrier molecules or bone compositions	1115:1186	Overall, three DBM products with different carrier molecules or bone compositions showed similar bone healing capacity.
24675830	0	8	theme	defect	88:93	arg1	model					95:99	a rabbit segmental defect model	69:99	a rabbit segmental defect model	69:99	Comparable bone healing capacity of different bone graft matrices in a rabbit segmental defect model.
24675830	1	9	theme	cortical	320:327	arg1	compositions					306:317	bone compositions	301:317	bone compositions (cortical bone vs. cortical bone and cancellous bone)	301:371	We compared the bone healing capacity of three different demineralized bone matrix (DBM) products applied using different carrier molecules (hyaluronic acid [HA] vs. carboxymethylcellulose [CMC]) or bone compositions (cortical bone vs. cortical bone and cancellous bone) in a rabbit segmental defect model.
24675830	1	9	theme	cortical	320:327	arg1	bone					329:332	cortical bone	320:332	cortical bone	320:332	We compared the bone healing capacity of three different demineralized bone matrix (DBM) products applied using different carrier molecules (hyaluronic acid [HA] vs. carboxymethylcellulose [CMC]) or bone compositions (cortical bone vs. cortical bone and cancellous bone) in a rabbit segmental defect model.
24675830	2	10	theme	HA-based	534:541	arg1	matrix					571:576	HA-based demineralized cortical bone matrix	534:576	HA-based demineralized cortical bone matrix (DBX)	534:582	Overall, 15-mm segmental defects in the left and right radiuses were created in 36 New Zealand White rabbits and filled with HA-based demineralized cortical bone matrix (DBX), CMC-based demineralized cortical bone matrix (DB) or CMC-based demineralized cortical bone with cancellous bone (NDDB), and the wound area was evaluated at 4, 8, and 12 weeks post-implantation.
24675830	2	10	theme	HA-based	534:541	arg1	DBX					579:581	DBX	579:581	DBX	579:581	Overall, 15-mm segmental defects in the left and right radiuses were created in 36 New Zealand White rabbits and filled with HA-based demineralized cortical bone matrix (DBX), CMC-based demineralized cortical bone matrix (DB) or CMC-based demineralized cortical bone with cancellous bone (NDDB), and the wound area was evaluated at 4, 8, and 12 weeks post-implantation.
24675830	2	11	theme	CMC-based	585:593	arg1	DB					631:632	DB	631:632	DB	631:632	Overall, 15-mm segmental defects in the left and right radiuses were created in 36 New Zealand White rabbits and filled with HA-based demineralized cortical bone matrix (DBX), CMC-based demineralized cortical bone matrix (DB) or CMC-based demineralized cortical bone with cancellous bone (NDDB), and the wound area was evaluated at 4, 8, and 12 weeks post-implantation.
24675830	2	11	theme	CMC-based	585:593	arg1	matrix					623:628	CMC-based demineralized cortical bone matrix	585:628	CMC-based demineralized cortical bone matrix (DB)	585:633	Overall, 15-mm segmental defects in the left and right radiuses were created in 36 New Zealand White rabbits and filled with HA-based demineralized cortical bone matrix (DBX), CMC-based demineralized cortical bone matrix (DB) or CMC-based demineralized cortical bone with cancellous bone (NDDB), and the wound area was evaluated at 4, 8, and 12 weeks post-implantation.
24675830	4	12	theme	volume	942:947	arg1	fraction					949:956	bone volume fraction	937:956	bone volume fraction	937:956	However, bone healing score, bone volume fraction, bone mineral density, and residual bone area at 4, 8, and 12 weeks post-implantation revealed no significant differences in bone healing capacity.
24675830	1	13	theme	hyaluronic	243:252	arg1	[HA					259:261	hyaluronic acid [HA] vs. carboxymethylcellulose [CMC	243:294	[HA	259:261	We compared the bone healing capacity of three different demineralized bone matrix (DBM) products applied using different carrier molecules (hyaluronic acid [HA] vs. carboxymethylcellulose [CMC]) or bone compositions (cortical bone vs. cortical bone and cancellous bone) in a rabbit segmental defect model.
24675830	0	14	theme	segmental	78:86	arg1	model					95:99	a rabbit segmental defect model	69:99	a rabbit segmental defect model	69:99	Comparable bone healing capacity of different bone graft matrices in a rabbit segmental defect model.
24675830	3	15	theme	bone	823:826	arg1	fraction					835:842	bone volume fraction	823:842	bone volume fraction	823:842	DBX showed significantly lower radiopacity, bone volume fraction, and bone mineral density than DB and NDDB before implantation.
24675830	5	16	theme	similar	1195:1201	arg1	capacity					1216:1223	similar bone healing capacity	1195:1223	similar bone healing capacity	1195:1223	Overall, three DBM products with different carrier molecules or bone compositions showed similar bone healing capacity.
24675830	1	17	theme	acid	254:257	arg1	[HA					259:261	hyaluronic acid [HA] vs. carboxymethylcellulose [CMC	243:294	[HA	259:261	We compared the bone healing capacity of three different demineralized bone matrix (DBM) products applied using different carrier molecules (hyaluronic acid [HA] vs. carboxymethylcellulose [CMC]) or bone compositions (cortical bone vs. cortical bone and cancellous bone) in a rabbit segmental defect model.
24675830	4	18	dep	score	930:934	arg1	post-implantation					1026:1042	post-implantation	1026:1042	post-implantation	1026:1042	However, bone healing score, bone volume fraction, bone mineral density, and residual bone area at 4, 8, and 12 weeks post-implantation revealed no significant differences in bone healing capacity.
24675830	5	19	theme	bone	1203:1206	arg1	capacity					1216:1223	similar bone healing capacity	1195:1223	similar bone healing capacity	1195:1223	Overall, three DBM products with different carrier molecules or bone compositions showed similar bone healing capacity.
24675830	1	20	theme	different	149:157	arg1	products					191:198	three different demineralized bone matrix (DBM) products	143:198	three different demineralized bone matrix (DBM) products applied	143:206	We compared the bone healing capacity of three different demineralized bone matrix (DBM) products applied using different carrier molecules (hyaluronic acid [HA] vs. carboxymethylcellulose [CMC]) or bone compositions (cortical bone vs. cortical bone and cancellous bone) in a rabbit segmental defect model.
24675830	2	21	theme	right	458:462	arg1	radiuses					464:471	the left and right radiuses	445:471	the left and right radiuses	445:471	Overall, 15-mm segmental defects in the left and right radiuses were created in 36 New Zealand White rabbits and filled with HA-based demineralized cortical bone matrix (DBX), CMC-based demineralized cortical bone matrix (DB) or CMC-based demineralized cortical bone with cancellous bone (NDDB), and the wound area was evaluated at 4, 8, and 12 weeks post-implantation.
24675830	1	22	theme	cortical	338:345	arg1	bone					347:350	cortical bone	338:350	cortical bone	338:350	We compared the bone healing capacity of three different demineralized bone matrix (DBM) products applied using different carrier molecules (hyaluronic acid [HA] vs. carboxymethylcellulose [CMC]) or bone compositions (cortical bone vs. cortical bone and cancellous bone) in a rabbit segmental defect model.
24675830	5	23	theme	bone	1170:1173	arg1	compositions					1175:1186	bone compositions	1170:1186	bone compositions	1170:1186	Overall, three DBM products with different carrier molecules or bone compositions showed similar bone healing capacity.
24675830	5	24	theme	healing	1208:1214	arg1	capacity					1216:1223	similar bone healing capacity	1195:1223	similar bone healing capacity	1195:1223	Overall, three DBM products with different carrier molecules or bone compositions showed similar bone healing capacity.
24675830	1	25	theme	demineralized	159:171	arg1	products					191:198	three different demineralized bone matrix (DBM) products	143:198	three different demineralized bone matrix (DBM) products applied	143:206	We compared the bone healing capacity of three different demineralized bone matrix (DBM) products applied using different carrier molecules (hyaluronic acid [HA] vs. carboxymethylcellulose [CMC]) or bone compositions (cortical bone vs. cortical bone and cancellous bone) in a rabbit segmental defect model.
24675830	0	26	theme	bone	11:14	arg1	capacity					24:31	Comparable bone healing capacity	0:31	Comparable bone healing capacity of different bone graft matrices in a rabbit segmental defect model.	0:100	Comparable bone healing capacity of different bone graft matrices in a rabbit segmental defect model.
24675830	1	27	dep	compositions	306:317	arg1	bone					367:370	cancellous bone	356:370	cancellous bone	356:370	We compared the bone healing capacity of three different demineralized bone matrix (DBM) products applied using different carrier molecules (hyaluronic acid [HA] vs. carboxymethylcellulose [CMC]) or bone compositions (cortical bone vs. cortical bone and cancellous bone) in a rabbit segmental defect model.
24675830	1	27	dep	compositions	306:317	arg1	compositions					306:317	bone compositions	301:317	bone compositions (cortical bone vs. cortical bone and cancellous bone)	301:371	We compared the bone healing capacity of three different demineralized bone matrix (DBM) products applied using different carrier molecules (hyaluronic acid [HA] vs. carboxymethylcellulose [CMC]) or bone compositions (cortical bone vs. cortical bone and cancellous bone) in a rabbit segmental defect model.
24675830	1	27	dep	compositions	306:317	arg1	bone					329:332	cortical bone	320:332	cortical bone	320:332	We compared the bone healing capacity of three different demineralized bone matrix (DBM) products applied using different carrier molecules (hyaluronic acid [HA] vs. carboxymethylcellulose [CMC]) or bone compositions (cortical bone vs. cortical bone and cancellous bone) in a rabbit segmental defect model.
24675830	1	27	dep	compositions	306:317	arg1	bone					347:350	cortical bone	338:350	cortical bone	338:350	We compared the bone healing capacity of three different demineralized bone matrix (DBM) products applied using different carrier molecules (hyaluronic acid [HA] vs. carboxymethylcellulose [CMC]) or bone compositions (cortical bone vs. cortical bone and cancellous bone) in a rabbit segmental defect model.
24675830	4	28	theme	significant	1056:1066	arg1	differences					1068:1078	no significant differences	1053:1078	no significant differences in bone healing capacity	1053:1103	However, bone healing score, bone volume fraction, bone mineral density, and residual bone area at 4, 8, and 12 weeks post-implantation revealed no significant differences in bone healing capacity.
24675830	2	29	theme	cancellous	681:690	arg1	NDDB					698:701	NDDB	698:701	NDDB	698:701	Overall, 15-mm segmental defects in the left and right radiuses were created in 36 New Zealand White rabbits and filled with HA-based demineralized cortical bone matrix (DBX), CMC-based demineralized cortical bone matrix (DB) or CMC-based demineralized cortical bone with cancellous bone (NDDB), and the wound area was evaluated at 4, 8, and 12 weeks post-implantation.
24675830	2	29	theme	cancellous	681:690	arg1	bone					692:695	cancellous bone	681:695	cancellous bone (NDDB)	681:702	Overall, 15-mm segmental defects in the left and right radiuses were created in 36 New Zealand White rabbits and filled with HA-based demineralized cortical bone matrix (DBX), CMC-based demineralized cortical bone matrix (DB) or CMC-based demineralized cortical bone with cancellous bone (NDDB), and the wound area was evaluated at 4, 8, and 12 weeks post-implantation.
24675830	1	30	theme	bone	173:176	arg1	products					191:198	three different demineralized bone matrix (DBM) products	143:198	three different demineralized bone matrix (DBM) products applied	143:206	We compared the bone healing capacity of three different demineralized bone matrix (DBM) products applied using different carrier molecules (hyaluronic acid [HA] vs. carboxymethylcellulose [CMC]) or bone compositions (cortical bone vs. cortical bone and cancellous bone) in a rabbit segmental defect model.
24675830	0	31	theme	Comparable	0:9	arg1	capacity					24:31	Comparable bone healing capacity	0:31	Comparable bone healing capacity of different bone graft matrices in a rabbit segmental defect model.	0:100	Comparable bone healing capacity of different bone graft matrices in a rabbit segmental defect model.
24675830	2	32	theme	bone	566:569	arg1	matrix					571:576	HA-based demineralized cortical bone matrix	534:576	HA-based demineralized cortical bone matrix (DBX)	534:582	Overall, 15-mm segmental defects in the left and right radiuses were created in 36 New Zealand White rabbits and filled with HA-based demineralized cortical bone matrix (DBX), CMC-based demineralized cortical bone matrix (DB) or CMC-based demineralized cortical bone with cancellous bone (NDDB), and the wound area was evaluated at 4, 8, and 12 weeks post-implantation.
24675830	2	32	theme	bone	566:569	arg1	DBX					579:581	DBX	579:581	DBX	579:581	Overall, 15-mm segmental defects in the left and right radiuses were created in 36 New Zealand White rabbits and filled with HA-based demineralized cortical bone matrix (DBX), CMC-based demineralized cortical bone matrix (DB) or CMC-based demineralized cortical bone with cancellous bone (NDDB), and the wound area was evaluated at 4, 8, and 12 weeks post-implantation.
24675830	2	33	theme	bone	618:621	arg1	DB					631:632	DB	631:632	DB	631:632	Overall, 15-mm segmental defects in the left and right radiuses were created in 36 New Zealand White rabbits and filled with HA-based demineralized cortical bone matrix (DBX), CMC-based demineralized cortical bone matrix (DB) or CMC-based demineralized cortical bone with cancellous bone (NDDB), and the wound area was evaluated at 4, 8, and 12 weeks post-implantation.
24675830	2	33	theme	bone	618:621	arg1	matrix					623:628	CMC-based demineralized cortical bone matrix	585:628	CMC-based demineralized cortical bone matrix (DB)	585:633	Overall, 15-mm segmental defects in the left and right radiuses were created in 36 New Zealand White rabbits and filled with HA-based demineralized cortical bone matrix (DBX), CMC-based demineralized cortical bone matrix (DB) or CMC-based demineralized cortical bone with cancellous bone (NDDB), and the wound area was evaluated at 4, 8, and 12 weeks post-implantation.
24675830	2	34	with	matrix	571:576	arg1	NDDB					698:701	NDDB	698:701	NDDB	698:701	Overall, 15-mm segmental defects in the left and right radiuses were created in 36 New Zealand White rabbits and filled with HA-based demineralized cortical bone matrix (DBX), CMC-based demineralized cortical bone matrix (DB) or CMC-based demineralized cortical bone with cancellous bone (NDDB), and the wound area was evaluated at 4, 8, and 12 weeks post-implantation.
24675830	2	34	with	matrix	571:576	arg1	bone					692:695	cancellous bone	681:695	cancellous bone (NDDB)	681:702	Overall, 15-mm segmental defects in the left and right radiuses were created in 36 New Zealand White rabbits and filled with HA-based demineralized cortical bone matrix (DBX), CMC-based demineralized cortical bone matrix (DB) or CMC-based demineralized cortical bone with cancellous bone (NDDB), and the wound area was evaluated at 4, 8, and 12 weeks post-implantation.
24675830	1	35	theme	matrix	178:183	arg1	products					191:198	three different demineralized bone matrix (DBM) products	143:198	three different demineralized bone matrix (DBM) products applied	143:206	We compared the bone healing capacity of three different demineralized bone matrix (DBM) products applied using different carrier molecules (hyaluronic acid [HA] vs. carboxymethylcellulose [CMC]) or bone compositions (cortical bone vs. cortical bone and cancellous bone) in a rabbit segmental defect model.
24675830	4	36	theme	bone	1083:1086	arg1	capacity					1096:1103	bone healing capacity	1083:1103	bone healing capacity	1083:1103	However, bone healing score, bone volume fraction, bone mineral density, and residual bone area at 4, 8, and 12 weeks post-implantation revealed no significant differences in bone healing capacity.
24675830	2	37	theme	Zealand	496:502	arg1	rabbits					510:516	36 New Zealand White rabbits	489:516	36 New Zealand White rabbits	489:516	Overall, 15-mm segmental defects in the left and right radiuses were created in 36 New Zealand White rabbits and filled with HA-based demineralized cortical bone matrix (DBX), CMC-based demineralized cortical bone matrix (DB) or CMC-based demineralized cortical bone with cancellous bone (NDDB), and the wound area was evaluated at 4, 8, and 12 weeks post-implantation.
24675830	2	38	theme	cortical	609:616	arg1	DB					631:632	DB	631:632	DB	631:632	Overall, 15-mm segmental defects in the left and right radiuses were created in 36 New Zealand White rabbits and filled with HA-based demineralized cortical bone matrix (DBX), CMC-based demineralized cortical bone matrix (DB) or CMC-based demineralized cortical bone with cancellous bone (NDDB), and the wound area was evaluated at 4, 8, and 12 weeks post-implantation.
24675830	2	38	theme	cortical	609:616	arg1	matrix					623:628	CMC-based demineralized cortical bone matrix	585:628	CMC-based demineralized cortical bone matrix (DB)	585:633	Overall, 15-mm segmental defects in the left and right radiuses were created in 36 New Zealand White rabbits and filled with HA-based demineralized cortical bone matrix (DBX), CMC-based demineralized cortical bone matrix (DB) or CMC-based demineralized cortical bone with cancellous bone (NDDB), and the wound area was evaluated at 4, 8, and 12 weeks post-implantation.
24675830	4	39	from	differences	1068:1078	arg1	capacity					1096:1103	bone healing capacity	1083:1103	bone healing capacity	1083:1103	However, bone healing score, bone volume fraction, bone mineral density, and residual bone area at 4, 8, and 12 weeks post-implantation revealed no significant differences in bone healing capacity.
24675830	2	40	theme	demineralized	595:607	arg1	DB					631:632	DB	631:632	DB	631:632	Overall, 15-mm segmental defects in the left and right radiuses were created in 36 New Zealand White rabbits and filled with HA-based demineralized cortical bone matrix (DBX), CMC-based demineralized cortical bone matrix (DB) or CMC-based demineralized cortical bone with cancellous bone (NDDB), and the wound area was evaluated at 4, 8, and 12 weeks post-implantation.
24675830	2	40	theme	demineralized	595:607	arg1	matrix					623:628	CMC-based demineralized cortical bone matrix	585:628	CMC-based demineralized cortical bone matrix (DB)	585:633	Overall, 15-mm segmental defects in the left and right radiuses were created in 36 New Zealand White rabbits and filled with HA-based demineralized cortical bone matrix (DBX), CMC-based demineralized cortical bone matrix (DB) or CMC-based demineralized cortical bone with cancellous bone (NDDB), and the wound area was evaluated at 4, 8, and 12 weeks post-implantation.
24675830	0	41	theme	healing	16:22	arg1	capacity					24:31	Comparable bone healing capacity	0:31	Comparable bone healing capacity of different bone graft matrices in a rabbit segmental defect model.	0:100	Comparable bone healing capacity of different bone graft matrices in a rabbit segmental defect model.
24675830	2	42	theme	New	492:494	arg1	rabbits					510:516	36 New Zealand White rabbits	489:516	36 New Zealand White rabbits	489:516	Overall, 15-mm segmental defects in the left and right radiuses were created in 36 New Zealand White rabbits and filled with HA-based demineralized cortical bone matrix (DBX), CMC-based demineralized cortical bone matrix (DB) or CMC-based demineralized cortical bone with cancellous bone (NDDB), and the wound area was evaluated at 4, 8, and 12 weeks post-implantation.
24675830	4	43	theme	residual	985:992	arg1	area					999:1002	residual bone area	985:1002	residual bone area at 4, 8, and 12 weeks	985:1024	However, bone healing score, bone volume fraction, bone mineral density, and residual bone area at 4, 8, and 12 weeks post-implantation revealed no significant differences in bone healing capacity.
24675830	2	44	theme	15-mm	418:422	arg1	defects					434:440	15-mm segmental defects	418:440	15-mm segmental defects in the left and right radiuses	418:471	Overall, 15-mm segmental defects in the left and right radiuses were created in 36 New Zealand White rabbits and filled with HA-based demineralized cortical bone matrix (DBX), CMC-based demineralized cortical bone matrix (DB) or CMC-based demineralized cortical bone with cancellous bone (NDDB), and the wound area was evaluated at 4, 8, and 12 weeks post-implantation.
24675830	1	45	theme	DBM	186:188	arg1	products					191:198	three different demineralized bone matrix (DBM) products	143:198	three different demineralized bone matrix (DBM) products applied	143:206	We compared the bone healing capacity of three different demineralized bone matrix (DBM) products applied using different carrier molecules (hyaluronic acid [HA] vs. carboxymethylcellulose [CMC]) or bone compositions (cortical bone vs. cortical bone and cancellous bone) in a rabbit segmental defect model.
24675830	0	46	theme	different	36:44	arg1	matrices					57:64	different bone graft matrices	36:64	different bone graft matrices	36:64	Comparable bone healing capacity of different bone graft matrices in a rabbit segmental defect model.
24675830	1	47	theme	carboxymethylcellulose	268:289	arg1	[CMC					291:294	hyaluronic acid [HA] vs. carboxymethylcellulose [CMC	243:294	[CMC	291:294	We compared the bone healing capacity of three different demineralized bone matrix (DBM) products applied using different carrier molecules (hyaluronic acid [HA] vs. carboxymethylcellulose [CMC]) or bone compositions (cortical bone vs. cortical bone and cancellous bone) in a rabbit segmental defect model.
24675830	1	48	theme	cancellous	356:365	arg1	bone					367:370	cancellous bone	356:370	cancellous bone	356:370	We compared the bone healing capacity of three different demineralized bone matrix (DBM) products applied using different carrier molecules (hyaluronic acid [HA] vs. carboxymethylcellulose [CMC]) or bone compositions (cortical bone vs. cortical bone and cancellous bone) in a rabbit segmental defect model.
24675830	1	48	theme	cancellous	356:365	arg1	compositions					306:317	bone compositions	301:317	bone compositions (cortical bone vs. cortical bone and cancellous bone)	301:371	We compared the bone healing capacity of three different demineralized bone matrix (DBM) products applied using different carrier molecules (hyaluronic acid [HA] vs. carboxymethylcellulose [CMC]) or bone compositions (cortical bone vs. cortical bone and cancellous bone) in a rabbit segmental defect model.
24675830	3	49	theme	volume	828:833	arg1	fraction					835:842	bone volume fraction	823:842	bone volume fraction	823:842	DBX showed significantly lower radiopacity, bone volume fraction, and bone mineral density than DB and NDDB before implantation.
24675830	2	50	theme	demineralized	543:555	arg1	matrix					571:576	HA-based demineralized cortical bone matrix	534:576	HA-based demineralized cortical bone matrix (DBX)	534:582	Overall, 15-mm segmental defects in the left and right radiuses were created in 36 New Zealand White rabbits and filled with HA-based demineralized cortical bone matrix (DBX), CMC-based demineralized cortical bone matrix (DB) or CMC-based demineralized cortical bone with cancellous bone (NDDB), and the wound area was evaluated at 4, 8, and 12 weeks post-implantation.
24675830	2	50	theme	demineralized	543:555	arg1	DBX					579:581	DBX	579:581	DBX	579:581	Overall, 15-mm segmental defects in the left and right radiuses were created in 36 New Zealand White rabbits and filled with HA-based demineralized cortical bone matrix (DBX), CMC-based demineralized cortical bone matrix (DB) or CMC-based demineralized cortical bone with cancellous bone (NDDB), and the wound area was evaluated at 4, 8, and 12 weeks post-implantation.
24675830	2	51	theme	segmental	424:432	arg1	defects					434:440	15-mm segmental defects	418:440	15-mm segmental defects in the left and right radiuses	418:471	Overall, 15-mm segmental defects in the left and right radiuses were created in 36 New Zealand White rabbits and filled with HA-based demineralized cortical bone matrix (DBX), CMC-based demineralized cortical bone matrix (DB) or CMC-based demineralized cortical bone with cancellous bone (NDDB), and the wound area was evaluated at 4, 8, and 12 weeks post-implantation.
24675830	2	52	theme	CMC-based	638:646	arg1	bone					671:674	CMC-based demineralized cortical bone	638:674	CMC-based demineralized cortical bone with cancellous bone (NDDB)	638:702	Overall, 15-mm segmental defects in the left and right radiuses were created in 36 New Zealand White rabbits and filled with HA-based demineralized cortical bone matrix (DBX), CMC-based demineralized cortical bone matrix (DB) or CMC-based demineralized cortical bone with cancellous bone (NDDB), and the wound area was evaluated at 4, 8, and 12 weeks post-implantation.
24675830	1	53	theme	products	191:198	arg1	capacity					131:138	the bone healing capacity	114:138	the bone healing capacity of three different demineralized bone matrix (DBM) products applied	114:206	We compared the bone healing capacity of three different demineralized bone matrix (DBM) products applied using different carrier molecules (hyaluronic acid [HA] vs. carboxymethylcellulose [CMC]) or bone compositions (cortical bone vs. cortical bone and cancellous bone) in a rabbit segmental defect model.
24675830	0	54	theme	graft	51:55	arg1	matrices					57:64	different bone graft matrices	36:64	different bone graft matrices	36:64	Comparable bone healing capacity of different bone graft matrices in a rabbit segmental defect model.
24675830	2	55	theme	left	449:452	arg1	radiuses					464:471	the left and right radiuses	445:471	the left and right radiuses	445:471	Overall, 15-mm segmental defects in the left and right radiuses were created in 36 New Zealand White rabbits and filled with HA-based demineralized cortical bone matrix (DBX), CMC-based demineralized cortical bone matrix (DB) or CMC-based demineralized cortical bone with cancellous bone (NDDB), and the wound area was evaluated at 4, 8, and 12 weeks post-implantation.
24675830	4	56	theme	bone	994:997	arg1	area					999:1002	residual bone area	985:1002	residual bone area at 4, 8, and 12 weeks	985:1024	However, bone healing score, bone volume fraction, bone mineral density, and residual bone area at 4, 8, and 12 weeks post-implantation revealed no significant differences in bone healing capacity.
24675830	2	57	from	defects	434:440	arg1	radiuses					464:471	the left and right radiuses	445:471	the left and right radiuses	445:471	Overall, 15-mm segmental defects in the left and right radiuses were created in 36 New Zealand White rabbits and filled with HA-based demineralized cortical bone matrix (DBX), CMC-based demineralized cortical bone matrix (DB) or CMC-based demineralized cortical bone with cancellous bone (NDDB), and the wound area was evaluated at 4, 8, and 12 weeks post-implantation.
24675830	0	58	theme	bone	46:49	arg1	matrices					57:64	different bone graft matrices	36:64	different bone graft matrices	36:64	Comparable bone healing capacity of different bone graft matrices in a rabbit segmental defect model.
24675830	1	59	theme	bone	301:304	arg1	bone					367:370	cancellous bone	356:370	cancellous bone	356:370	We compared the bone healing capacity of three different demineralized bone matrix (DBM) products applied using different carrier molecules (hyaluronic acid [HA] vs. carboxymethylcellulose [CMC]) or bone compositions (cortical bone vs. cortical bone and cancellous bone) in a rabbit segmental defect model.
24675830	1	59	theme	bone	301:304	arg1	compositions					306:317	bone compositions	301:317	bone compositions (cortical bone vs. cortical bone and cancellous bone)	301:371	We compared the bone healing capacity of three different demineralized bone matrix (DBM) products applied using different carrier molecules (hyaluronic acid [HA] vs. carboxymethylcellulose [CMC]) or bone compositions (cortical bone vs. cortical bone and cancellous bone) in a rabbit segmental defect model.
24675830	1	59	theme	bone	301:304	arg1	bone					329:332	cortical bone	320:332	cortical bone	320:332	We compared the bone healing capacity of three different demineralized bone matrix (DBM) products applied using different carrier molecules (hyaluronic acid [HA] vs. carboxymethylcellulose [CMC]) or bone compositions (cortical bone vs. cortical bone and cancellous bone) in a rabbit segmental defect model.
24675830	4	60	from	weeks	1020:1024	arg1	density					972:978	bone mineral density	959:978	bone mineral density	959:978	However, bone healing score, bone volume fraction, bone mineral density, and residual bone area at 4, 8, and 12 weeks post-implantation revealed no significant differences in bone healing capacity.
24675830	4	60	from	weeks	1020:1024	arg1	area					999:1002	residual bone area	985:1002	residual bone area at 4, 8, and 12 weeks	985:1024	However, bone healing score, bone volume fraction, bone mineral density, and residual bone area at 4, 8, and 12 weeks post-implantation revealed no significant differences in bone healing capacity.
24675830	4	60	from	weeks	1020:1024	arg1	fraction					949:956	bone volume fraction	937:956	bone volume fraction	937:956	However, bone healing score, bone volume fraction, bone mineral density, and residual bone area at 4, 8, and 12 weeks post-implantation revealed no significant differences in bone healing capacity.
24675830	4	60	from	weeks	1020:1024	arg1	score					930:934	bone healing score	917:934	bone healing score	917:934	However, bone healing score, bone volume fraction, bone mineral density, and residual bone area at 4, 8, and 12 weeks post-implantation revealed no significant differences in bone healing capacity.
24675830	4	61	theme	healing	1088:1094	arg1	capacity					1096:1103	bone healing capacity	1083:1103	bone healing capacity	1083:1103	However, bone healing score, bone volume fraction, bone mineral density, and residual bone area at 4, 8, and 12 weeks post-implantation revealed no significant differences in bone healing capacity.
24675830	4	62	theme	mineral	964:970	arg1	density					972:978	bone mineral density	959:978	bone mineral density	959:978	However, bone healing score, bone volume fraction, bone mineral density, and residual bone area at 4, 8, and 12 weeks post-implantation revealed no significant differences in bone healing capacity.
24675830	4	63	theme	bone	959:962	arg1	density					972:978	bone mineral density	959:978	bone mineral density	959:978	However, bone healing score, bone volume fraction, bone mineral density, and residual bone area at 4, 8, and 12 weeks post-implantation revealed no significant differences in bone healing capacity.
24675830	3	64	theme	bone	849:852	arg1	density					862:868	bone mineral density	849:868	bone mineral density	849:868	DBX showed significantly lower radiopacity, bone volume fraction, and bone mineral density than DB and NDDB before implantation.
24675830	5	65	theme	different	1139:1147	arg1	molecules					1157:1165	different carrier molecules	1139:1165	different carrier molecules	1139:1165	Overall, three DBM products with different carrier molecules or bone compositions showed similar bone healing capacity.
24675830	2	66	theme	weeks	754:758	arg1	post-implantation					760:776	4, 8, and 12 weeks post-implantation	741:776	4, 8, and 12 weeks post-implantation	741:776	Overall, 15-mm segmental defects in the left and right radiuses were created in 36 New Zealand White rabbits and filled with HA-based demineralized cortical bone matrix (DBX), CMC-based demineralized cortical bone matrix (DB) or CMC-based demineralized cortical bone with cancellous bone (NDDB), and the wound area was evaluated at 4, 8, and 12 weeks post-implantation.
24675830	2	67	theme	White	504:508	arg1	rabbits					510:516	36 New Zealand White rabbits	489:516	36 New Zealand White rabbits	489:516	Overall, 15-mm segmental defects in the left and right radiuses were created in 36 New Zealand White rabbits and filled with HA-based demineralized cortical bone matrix (DBX), CMC-based demineralized cortical bone matrix (DB) or CMC-based demineralized cortical bone with cancellous bone (NDDB), and the wound area was evaluated at 4, 8, and 12 weeks post-implantation.
24675830	1	68	theme	rabbit	378:383	arg1	model					402:406	a rabbit segmental defect model	376:406	a rabbit segmental defect model	376:406	We compared the bone healing capacity of three different demineralized bone matrix (DBM) products applied using different carrier molecules (hyaluronic acid [HA] vs. carboxymethylcellulose [CMC]) or bone compositions (cortical bone vs. cortical bone and cancellous bone) in a rabbit segmental defect model.
24675830	3	69	theme	mineral	854:860	arg1	density					862:868	bone mineral density	849:868	bone mineral density	849:868	DBX showed significantly lower radiopacity, bone volume fraction, and bone mineral density than DB and NDDB before implantation.
24675830	1	70	theme	bone	118:121	arg1	capacity					131:138	the bone healing capacity	114:138	the bone healing capacity of three different demineralized bone matrix (DBM) products applied	114:206	We compared the bone healing capacity of three different demineralized bone matrix (DBM) products applied using different carrier molecules (hyaluronic acid [HA] vs. carboxymethylcellulose [CMC]) or bone compositions (cortical bone vs. cortical bone and cancellous bone) in a rabbit segmental defect model.
24675830	5	71	theme	carrier	1149:1155	arg1	molecules					1157:1165	different carrier molecules	1139:1165	different carrier molecules	1139:1165	Overall, three DBM products with different carrier molecules or bone compositions showed similar bone healing capacity.
24675830	2	72	with	bone	671:674	arg1	NDDB					698:701	NDDB	698:701	NDDB	698:701	Overall, 15-mm segmental defects in the left and right radiuses were created in 36 New Zealand White rabbits and filled with HA-based demineralized cortical bone matrix (DBX), CMC-based demineralized cortical bone matrix (DB) or CMC-based demineralized cortical bone with cancellous bone (NDDB), and the wound area was evaluated at 4, 8, and 12 weeks post-implantation.
24675830	2	72	with	bone	671:674	arg1	bone					692:695	cancellous bone	681:695	cancellous bone (NDDB)	681:702	Overall, 15-mm segmental defects in the left and right radiuses were created in 36 New Zealand White rabbits and filled with HA-based demineralized cortical bone matrix (DBX), CMC-based demineralized cortical bone matrix (DB) or CMC-based demineralized cortical bone with cancellous bone (NDDB), and the wound area was evaluated at 4, 8, and 12 weeks post-implantation.
24675830	1	73	theme	segmental	385:393	arg1	model					402:406	a rabbit segmental defect model	376:406	a rabbit segmental defect model	376:406	We compared the bone healing capacity of three different demineralized bone matrix (DBM) products applied using different carrier molecules (hyaluronic acid [HA] vs. carboxymethylcellulose [CMC]) or bone compositions (cortical bone vs. cortical bone and cancellous bone) in a rabbit segmental defect model.
24675830	2	74	with	matrix	623:628	arg1	NDDB					698:701	NDDB	698:701	NDDB	698:701	Overall, 15-mm segmental defects in the left and right radiuses were created in 36 New Zealand White rabbits and filled with HA-based demineralized cortical bone matrix (DBX), CMC-based demineralized cortical bone matrix (DB) or CMC-based demineralized cortical bone with cancellous bone (NDDB), and the wound area was evaluated at 4, 8, and 12 weeks post-implantation.
24675830	2	74	with	matrix	623:628	arg1	bone					692:695	cancellous bone	681:695	cancellous bone (NDDB)	681:702	Overall, 15-mm segmental defects in the left and right radiuses were created in 36 New Zealand White rabbits and filled with HA-based demineralized cortical bone matrix (DBX), CMC-based demineralized cortical bone matrix (DB) or CMC-based demineralized cortical bone with cancellous bone (NDDB), and the wound area was evaluated at 4, 8, and 12 weeks post-implantation.
24675830	1	75	theme	healing	123:129	arg1	capacity					131:138	the bone healing capacity	114:138	the bone healing capacity of three different demineralized bone matrix (DBM) products applied	114:206	We compared the bone healing capacity of three different demineralized bone matrix (DBM) products applied using different carrier molecules (hyaluronic acid [HA] vs. carboxymethylcellulose [CMC]) or bone compositions (cortical bone vs. cortical bone and cancellous bone) in a rabbit segmental defect model.
24675830	2	76	theme	wound	713:717	arg1	area					719:722	the wound area	709:722	the wound area	709:722	Overall, 15-mm segmental defects in the left and right radiuses were created in 36 New Zealand White rabbits and filled with HA-based demineralized cortical bone matrix (DBX), CMC-based demineralized cortical bone matrix (DB) or CMC-based demineralized cortical bone with cancellous bone (NDDB), and the wound area was evaluated at 4, 8, and 12 weeks post-implantation.
24675830	1	77	theme	different	214:222	arg1	molecules					232:240	different carrier molecules	214:240	different carrier molecules (hyaluronic acid [HA] vs. carboxymethylcellulose [CMC])	214:296	We compared the bone healing capacity of three different demineralized bone matrix (DBM) products applied using different carrier molecules (hyaluronic acid [HA] vs. carboxymethylcellulose [CMC]) or bone compositions (cortical bone vs. cortical bone and cancellous bone) in a rabbit segmental defect model.
24675830	0	78	theme	matrices	57:64	arg1	capacity					24:31	Comparable bone healing capacity	0:31	Comparable bone healing capacity of different bone graft matrices in a rabbit segmental defect model.	0:100	Comparable bone healing capacity of different bone graft matrices in a rabbit segmental defect model.
24675830	0	79	from	capacity	24:31	arg1	model					95:99	a rabbit segmental defect model	69:99	a rabbit segmental defect model	69:99	Comparable bone healing capacity of different bone graft matrices in a rabbit segmental defect model.
24675830	4	80	theme	healing	922:928	arg1	score					930:934	bone healing score	917:934	bone healing score	917:934	However, bone healing score, bone volume fraction, bone mineral density, and residual bone area at 4, 8, and 12 weeks post-implantation revealed no significant differences in bone healing capacity.
24675830	2	81	theme	cortical	662:669	arg1	bone					671:674	CMC-based demineralized cortical bone	638:674	CMC-based demineralized cortical bone with cancellous bone (NDDB)	638:702	Overall, 15-mm segmental defects in the left and right radiuses were created in 36 New Zealand White rabbits and filled with HA-based demineralized cortical bone matrix (DBX), CMC-based demineralized cortical bone matrix (DB) or CMC-based demineralized cortical bone with cancellous bone (NDDB), and the wound area was evaluated at 4, 8, and 12 weeks post-implantation.
24675830	4	82	theme	bone	917:920	arg1	score					930:934	bone healing score	917:934	bone healing score	917:934	However, bone healing score, bone volume fraction, bone mineral density, and residual bone area at 4, 8, and 12 weeks post-implantation revealed no significant differences in bone healing capacity.
24675830	4	83	theme	bone	937:940	arg1	fraction					949:956	bone volume fraction	937:956	bone volume fraction	937:956	However, bone healing score, bone volume fraction, bone mineral density, and residual bone area at 4, 8, and 12 weeks post-implantation revealed no significant differences in bone healing capacity.
24675830	1	84	theme	carrier	224:230	arg1	molecules					232:240	different carrier molecules	214:240	different carrier molecules (hyaluronic acid [HA] vs. carboxymethylcellulose [CMC])	214:296	We compared the bone healing capacity of three different demineralized bone matrix (DBM) products applied using different carrier molecules (hyaluronic acid [HA] vs. carboxymethylcellulose [CMC]) or bone compositions (cortical bone vs. cortical bone and cancellous bone) in a rabbit segmental defect model.
28991204	6	0	theme	internal	919:926	arg1	volatiles					928:936	some internal volatiles	914:936	some internal volatiles	914:936	The amounts of polysaccharides and some internal volatiles were significantly decreased by PGR treatment; while the free amino acids content was generally increased.
28991204	2	1	used	used	241:244	arg2	They					225:228	They	225:228	They	225:228	They are widely used in the cultivation of rhizomatous functional plants; such as Codonopsis Radix, that is a famous Chinese traditional herb.
28991204	1	2	theme	cell	219:222	arg1	division					143:150	the cell division	134:150	the cell division of plant stem tip sub-apical meristem cells or primordial meristem cell	134:222	Plant growth retardant (PGR) refers to organics that can inhibit the cell division of plant stem tip sub-apical meristem cells or primordial meristem cell.
28991204	2	3	theme	Chinese	342:348	arg1	herb					362:365	a famous Chinese traditional herb	333:365	a famous Chinese traditional herb	333:365	They are widely used in the cultivation of rhizomatous functional plants; such as Codonopsis Radix, that is a famous Chinese traditional herb.
28991204	2	3	theme	Chinese	342:348	arg1	Radix					318:322	Codonopsis Radix	307:322	Codonopsis Radix	307:322	They are widely used in the cultivation of rhizomatous functional plants; such as Codonopsis Radix, that is a famous Chinese traditional herb.
28991204	2	4	theme	Codonopsis	307:316	arg1	Radix					318:322	Codonopsis Radix	307:322	Codonopsis Radix	307:322	They are widely used in the cultivation of rhizomatous functional plants; such as Codonopsis Radix, that is a famous Chinese traditional herb.
28991204	2	4	theme	Codonopsis	307:316	arg1	herb					362:365	a famous Chinese traditional herb	333:365	a famous Chinese traditional herb	333:365	They are widely used in the cultivation of rhizomatous functional plants; such as Codonopsis Radix, that is a famous Chinese traditional herb.
28991204	4	5	theme	targeted	495:502	arg1	analyses					521:528	targeted and non-targeted analyses	495:528	targeted and non-targeted analyses by ultra-performance liquid chromatography combined with time-of-flight mass spectrometry (UPLC-TOF-MS)	495:632	In the present study, amino acid analyses, targeted and non-targeted analyses by ultra-performance liquid chromatography combined with time-of-flight mass spectrometry (UPLC-TOF-MS) and gas chromatography-MS were used to analyze and compare the composition of untreated C. Radix and C. Radix treated with PGR.
28991204	0	6	from	Influence	0:8	arg1	Quality					40:46	Quality	40:46	Quality of Codonopsis Radix	40:66	Influence of Plant Growth Retardants on Quality of Codonopsis Radix.
28991204	6	7	theme	volatiles	928:936	arg1	polysaccharides					894:908	polysaccharides	894:908	polysaccharides	894:908	The amounts of polysaccharides and some internal volatiles were significantly decreased by PGR treatment; while the free amino acids content was generally increased.
28991204	6	7	theme	volatiles	928:936	arg1	amounts					883:889	The amounts	879:889	The amounts of polysaccharides and some internal volatiles	879:936	The amounts of polysaccharides and some internal volatiles were significantly decreased by PGR treatment; while the free amino acids content was generally increased.
28991204	6	7	theme	volatiles	928:936	arg1	volatiles					928:936	some internal volatiles	914:936	some internal volatiles	914:936	The amounts of polysaccharides and some internal volatiles were significantly decreased by PGR treatment; while the free amino acids content was generally increased.
28991204	2	8	theme	functional	280:289	arg1	Radix					318:322	Codonopsis Radix	307:322	Codonopsis Radix	307:322	They are widely used in the cultivation of rhizomatous functional plants; such as Codonopsis Radix, that is a famous Chinese traditional herb.
28991204	2	8	theme	functional	280:289	arg1	plants					291:296	rhizomatous functional plants	268:296	rhizomatous functional plants; such as Codonopsis Radix, that is a famous Chinese traditional herb	268:365	They are widely used in the cultivation of rhizomatous functional plants; such as Codonopsis Radix, that is a famous Chinese traditional herb.
28991204	4	9	used	used	665:668	arg2	analyses					485:492	amino acid analyses	474:492	amino acid analyses	474:492	In the present study, amino acid analyses, targeted and non-targeted analyses by ultra-performance liquid chromatography combined with time-of-flight mass spectrometry (UPLC-TOF-MS) and gas chromatography-MS were used to analyze and compare the composition of untreated C. Radix and C. Radix treated with PGR.
28991204	4	9	used	used	665:668	arg2	analyses					521:528	targeted and non-targeted analyses	495:528	targeted and non-targeted analyses by ultra-performance liquid chromatography combined with time-of-flight mass spectrometry (UPLC-TOF-MS)	495:632	In the present study, amino acid analyses, targeted and non-targeted analyses by ultra-performance liquid chromatography combined with time-of-flight mass spectrometry (UPLC-TOF-MS) and gas chromatography-MS were used to analyze and compare the composition of untreated C. Radix and C. Radix treated with PGR.
28991204	4	9	used	used	665:668	arg2	chromatography-MS					642:658	gas chromatography-MS	638:658	gas chromatography-MS	638:658	In the present study, amino acid analyses, targeted and non-targeted analyses by ultra-performance liquid chromatography combined with time-of-flight mass spectrometry (UPLC-TOF-MS) and gas chromatography-MS were used to analyze and compare the composition of untreated C. Radix and C. Radix treated with PGR.
28991204	8	10	theme	buqi	1272:1275	arg1	"					1276:1276	"buqi"	1271:1276	"buqi"	1271:1276	Five of the up-regulated compounds have been reported to show immune activity, which might contribute to the healing efficacy ("buqi") of C. Radix.
28991204	8	10	theme	buqi	1272:1275	arg1	efficacy					1261:1268	healing efficacy	1253:1268	the healing efficacy ("buqi") of C. Radix	1249:1289	Five of the up-regulated compounds have been reported to show immune activity, which might contribute to the healing efficacy ("buqi") of C. Radix.
28991204	3	11	theme	medicinal	421:429	arg1	quality					431:437	the medicinal quality	417:437	the medicinal quality of C. Radix	417:449	However, it is still unclear whether PGR affects the medicinal quality of C. Radix.
28991204	5	12	theme	key	782:784	arg1	atractylenolide					822:836	atractylenolide III	822:840	atractylenolide III	822:840	The contents of two key bioactive compounds, lobetyolin and atractylenolide III, were not affected by PGR treatment.
28991204	5	12	theme	key	782:784	arg1	lobetyolin					807:816	lobetyolin	807:816	lobetyolin	807:816	The contents of two key bioactive compounds, lobetyolin and atractylenolide III, were not affected by PGR treatment.
28991204	5	12	theme	key	782:784	arg1	compounds					796:804	two key bioactive compounds	778:804	two key bioactive compounds	778:804	The contents of two key bioactive compounds, lobetyolin and atractylenolide III, were not affected by PGR treatment.
28991204	8	13	theme	immune	1206:1211	arg1	activity					1213:1220	immune activity	1206:1220	immune activity	1206:1220	Five of the up-regulated compounds have been reported to show immune activity, which might contribute to the healing efficacy ("buqi") of C. Radix.
28991204	1	14	theme	cell	138:141	arg1	division					143:150	the cell division	134:150	the cell division of plant stem tip sub-apical meristem cells or primordial meristem cell	134:222	Plant growth retardant (PGR) refers to organics that can inhibit the cell division of plant stem tip sub-apical meristem cells or primordial meristem cell.
28991204	4	15	theme	non-targeted	508:519	arg1	analyses					521:528	targeted and non-targeted analyses	495:528	targeted and non-targeted analyses by ultra-performance liquid chromatography combined with time-of-flight mass spectrometry (UPLC-TOF-MS)	495:632	In the present study, amino acid analyses, targeted and non-targeted analyses by ultra-performance liquid chromatography combined with time-of-flight mass spectrometry (UPLC-TOF-MS) and gas chromatography-MS were used to analyze and compare the composition of untreated C. Radix and C. Radix treated with PGR.
28991204	8	16	theme	compounds	1169:1177	arg1	compounds					1169:1177	the up-regulated compounds	1152:1177	the up-regulated compounds	1152:1177	Five of the up-regulated compounds have been reported to show immune activity, which might contribute to the healing efficacy ("buqi") of C. Radix.
28991204	8	16	theme	compounds	1169:1177	arg1	Five					1144:1147	Five	1144:1147	Five	1144:1147	Five of the up-regulated compounds have been reported to show immune activity, which might contribute to the healing efficacy ("buqi") of C. Radix.
28991204	9	17	theme	bioactive	1425:1433	arg1	compounds					1435:1443	some main bioactive compounds	1415:1443	some main bioactive compounds	1415:1443	The results of this study showed that treatment of C. Radix with PGR during cultivation has economic benefits and affected some main bioactive compounds in C. Radix.
28991204	1	18	theme	Plant	69:73	arg1	retardant					82:90	Plant growth retardant	69:90	Plant growth retardant (PGR)	69:96	Plant growth retardant (PGR) refers to organics that can inhibit the cell division of plant stem tip sub-apical meristem cells or primordial meristem cell.
28991204	1	18	theme	Plant	69:73	arg1	PGR					93:95	PGR	93:95	PGR	93:95	Plant growth retardant (PGR) refers to organics that can inhibit the cell division of plant stem tip sub-apical meristem cells or primordial meristem cell.
28991204	2	19	theme	famous	335:340	arg1	herb					362:365	a famous Chinese traditional herb	333:365	a famous Chinese traditional herb	333:365	They are widely used in the cultivation of rhizomatous functional plants; such as Codonopsis Radix, that is a famous Chinese traditional herb.
28991204	2	19	theme	famous	335:340	arg1	Radix					318:322	Codonopsis Radix	307:322	Codonopsis Radix	307:322	They are widely used in the cultivation of rhizomatous functional plants; such as Codonopsis Radix, that is a famous Chinese traditional herb.
28991204	9	20	contain	has	1380:1382	arg2	benefits					1393:1400	economic benefits	1384:1400	economic benefits	1384:1400	The results of this study showed that treatment of C. Radix with PGR during cultivation has economic benefits and affected some main bioactive compounds in C. Radix.
28991204	9	20	contain	has	1380:1382	arg1	treatment					1330:1338	treatment	1330:1338	treatment of C. Radix with PGR during cultivation	1330:1378	The results of this study showed that treatment of C. Radix with PGR during cultivation has economic benefits and affected some main bioactive compounds in C. Radix.
28991204	1	21	theme	growth	75:80	arg1	retardant					82:90	Plant growth retardant	69:90	Plant growth retardant (PGR)	69:96	Plant growth retardant (PGR) refers to organics that can inhibit the cell division of plant stem tip sub-apical meristem cells or primordial meristem cell.
28991204	1	21	theme	growth	75:80	arg1	PGR					93:95	PGR	93:95	PGR	93:95	Plant growth retardant (PGR) refers to organics that can inhibit the cell division of plant stem tip sub-apical meristem cells or primordial meristem cell.
28991204	6	22	theme	acids	1006:1010	arg1	content					1012:1018	the free amino acids content	991:1018	the free amino acids content	991:1018	The amounts of polysaccharides and some internal volatiles were significantly decreased by PGR treatment; while the free amino acids content was generally increased.
28991204	4	23	theme	acid	480:483	arg1	analyses					485:492	amino acid analyses	474:492	amino acid analyses	474:492	In the present study, amino acid analyses, targeted and non-targeted analyses by ultra-performance liquid chromatography combined with time-of-flight mass spectrometry (UPLC-TOF-MS) and gas chromatography-MS were used to analyze and compare the composition of untreated C. Radix and C. Radix treated with PGR.
28991204	5	24	theme	compounds	796:804	arg1	contents					766:773	The contents	762:773	The contents of two key bioactive compounds, lobetyolin and atractylenolide III,	762:841	The contents of two key bioactive compounds, lobetyolin and atractylenolide III, were not affected by PGR treatment.
28991204	2	25	theme	rhizomatous	268:278	arg1	Radix					318:322	Codonopsis Radix	307:322	Codonopsis Radix	307:322	They are widely used in the cultivation of rhizomatous functional plants; such as Codonopsis Radix, that is a famous Chinese traditional herb.
28991204	2	25	theme	rhizomatous	268:278	arg1	plants					291:296	rhizomatous functional plants	268:296	rhizomatous functional plants; such as Codonopsis Radix, that is a famous Chinese traditional herb	268:365	They are widely used in the cultivation of rhizomatous functional plants; such as Codonopsis Radix, that is a famous Chinese traditional herb.
28991204	1	26	theme	plant	155:159	arg1	cells					190:194	plant stem tip sub-apical meristem cells	155:194	plant stem tip sub-apical meristem cells	155:194	Plant growth retardant (PGR) refers to organics that can inhibit the cell division of plant stem tip sub-apical meristem cells or primordial meristem cell.
28991204	0	27	theme	Growth	19:24	arg1	Retardants					26:35	Plant Growth Retardants	13:35	Plant Growth Retardants	13:35	Influence of Plant Growth Retardants on Quality of Codonopsis Radix.
28991204	4	28	theme	amino	474:478	arg1	analyses					485:492	amino acid analyses	474:492	amino acid analyses	474:492	In the present study, amino acid analyses, targeted and non-targeted analyses by ultra-performance liquid chromatography combined with time-of-flight mass spectrometry (UPLC-TOF-MS) and gas chromatography-MS were used to analyze and compare the composition of untreated C. Radix and C. Radix treated with PGR.
28991204	5	29	theme	PGR	864:866	arg1	treatment					868:876	PGR treatment	864:876	PGR treatment	864:876	The contents of two key bioactive compounds, lobetyolin and atractylenolide III, were not affected by PGR treatment.
28991204	1	30	theme	stem	161:164	arg1	cells					190:194	plant stem tip sub-apical meristem cells	155:194	plant stem tip sub-apical meristem cells	155:194	Plant growth retardant (PGR) refers to organics that can inhibit the cell division of plant stem tip sub-apical meristem cells or primordial meristem cell.
28991204	0	31	theme	Plant	13:17	arg1	Retardants					26:35	Plant Growth Retardants	13:35	Plant Growth Retardants	13:35	Influence of Plant Growth Retardants on Quality of Codonopsis Radix.
28991204	6	32	theme	polysaccharides	894:908	arg1	polysaccharides					894:908	polysaccharides	894:908	polysaccharides	894:908	The amounts of polysaccharides and some internal volatiles were significantly decreased by PGR treatment; while the free amino acids content was generally increased.
28991204	6	32	theme	polysaccharides	894:908	arg1	amounts					883:889	The amounts	879:889	The amounts of polysaccharides and some internal volatiles	879:936	The amounts of polysaccharides and some internal volatiles were significantly decreased by PGR treatment; while the free amino acids content was generally increased.
28991204	6	32	theme	polysaccharides	894:908	arg1	volatiles					928:936	some internal volatiles	914:936	some internal volatiles	914:936	The amounts of polysaccharides and some internal volatiles were significantly decreased by PGR treatment; while the free amino acids content was generally increased.
28991204	3	33	theme	Radix	445:449	arg1	quality					431:437	the medicinal quality	417:437	the medicinal quality of C. Radix	417:449	However, it is still unclear whether PGR affects the medicinal quality of C. Radix.
28991204	1	34	theme	tip	166:168	arg1	cells					190:194	plant stem tip sub-apical meristem cells	155:194	plant stem tip sub-apical meristem cells	155:194	Plant growth retardant (PGR) refers to organics that can inhibit the cell division of plant stem tip sub-apical meristem cells or primordial meristem cell.
28991204	7	35	theme	PGR	1098:1100	arg1	treatment					1102:1110	PGR treatment	1098:1110	PGR treatment	1098:1110	Fifteen metabolites whose abundance were affected by PGR treatment were identified by UPLC-TOF-MS.
28991204	4	36	theme	mass	602:605	arg1	UPLC-TOF-MS					621:631	UPLC-TOF-MS	621:631	UPLC-TOF-MS	621:631	In the present study, amino acid analyses, targeted and non-targeted analyses by ultra-performance liquid chromatography combined with time-of-flight mass spectrometry (UPLC-TOF-MS) and gas chromatography-MS were used to analyze and compare the composition of untreated C. Radix and C. Radix treated with PGR.
28991204	4	36	theme	mass	602:605	arg1	spectrometry					607:618	time-of-flight mass spectrometry	587:618	time-of-flight mass spectrometry (UPLC-TOF-MS)	587:632	In the present study, amino acid analyses, targeted and non-targeted analyses by ultra-performance liquid chromatography combined with time-of-flight mass spectrometry (UPLC-TOF-MS) and gas chromatography-MS were used to analyze and compare the composition of untreated C. Radix and C. Radix treated with PGR.
28991204	4	37	theme	time-of-flight	587:600	arg1	UPLC-TOF-MS					621:631	UPLC-TOF-MS	621:631	UPLC-TOF-MS	621:631	In the present study, amino acid analyses, targeted and non-targeted analyses by ultra-performance liquid chromatography combined with time-of-flight mass spectrometry (UPLC-TOF-MS) and gas chromatography-MS were used to analyze and compare the composition of untreated C. Radix and C. Radix treated with PGR.
28991204	4	37	theme	time-of-flight	587:600	arg1	spectrometry					607:618	time-of-flight mass spectrometry	587:618	time-of-flight mass spectrometry (UPLC-TOF-MS)	587:632	In the present study, amino acid analyses, targeted and non-targeted analyses by ultra-performance liquid chromatography combined with time-of-flight mass spectrometry (UPLC-TOF-MS) and gas chromatography-MS were used to analyze and compare the composition of untreated C. Radix and C. Radix treated with PGR.
28991204	6	38	theme	amino	1000:1004	arg1	acids					1006:1010	the free amino acids	991:1010	the free amino acids content	991:1018	The amounts of polysaccharides and some internal volatiles were significantly decreased by PGR treatment; while the free amino acids content was generally increased.
28991204	1	39	theme	sub-apical	170:179	arg1	cells					190:194	plant stem tip sub-apical meristem cells	155:194	plant stem tip sub-apical meristem cells	155:194	Plant growth retardant (PGR) refers to organics that can inhibit the cell division of plant stem tip sub-apical meristem cells or primordial meristem cell.
28991204	0	40	theme	Retardants	26:35	arg1	Influence					0:8	Influence	0:8	Influence of Plant Growth Retardants on Quality of Codonopsis Radix.	0:67	Influence of Plant Growth Retardants on Quality of Codonopsis Radix.
28991204	4	41	theme	present	459:465	arg1	study					467:471	the present study	455:471	the present study	455:471	In the present study, amino acid analyses, targeted and non-targeted analyses by ultra-performance liquid chromatography combined with time-of-flight mass spectrometry (UPLC-TOF-MS) and gas chromatography-MS were used to analyze and compare the composition of untreated C. Radix and C. Radix treated with PGR.
28991204	8	42	theme	up-regulated	1156:1167	arg1	compounds					1169:1177	the up-regulated compounds	1152:1177	the up-regulated compounds	1152:1177	Five of the up-regulated compounds have been reported to show immune activity, which might contribute to the healing efficacy ("buqi") of C. Radix.
28991204	9	43	theme	study	1312:1316	arg1	results					1296:1302	The results	1292:1302	The results of this study	1292:1316	The results of this study showed that treatment of C. Radix with PGR during cultivation has economic benefits and affected some main bioactive compounds in C. Radix.
28991204	5	44	theme	bioactive	786:794	arg1	atractylenolide					822:836	atractylenolide III	822:840	atractylenolide III	822:840	The contents of two key bioactive compounds, lobetyolin and atractylenolide III, were not affected by PGR treatment.
28991204	5	44	theme	bioactive	786:794	arg1	lobetyolin					807:816	lobetyolin	807:816	lobetyolin	807:816	The contents of two key bioactive compounds, lobetyolin and atractylenolide III, were not affected by PGR treatment.
28991204	5	44	theme	bioactive	786:794	arg1	compounds					796:804	two key bioactive compounds	778:804	two key bioactive compounds	778:804	The contents of two key bioactive compounds, lobetyolin and atractylenolide III, were not affected by PGR treatment.
28991204	1	45	theme	meristem	181:188	arg1	cells					190:194	plant stem tip sub-apical meristem cells	155:194	plant stem tip sub-apical meristem cells	155:194	Plant growth retardant (PGR) refers to organics that can inhibit the cell division of plant stem tip sub-apical meristem cells or primordial meristem cell.
28991204	9	46	theme	economic	1384:1391	arg1	benefits					1393:1400	economic benefits	1384:1400	economic benefits	1384:1400	The results of this study showed that treatment of C. Radix with PGR during cultivation has economic benefits and affected some main bioactive compounds in C. Radix.
28991204	8	47	theme	Radix	1285:1289	arg1	"					1276:1276	"buqi"	1271:1276	"buqi"	1271:1276	Five of the up-regulated compounds have been reported to show immune activity, which might contribute to the healing efficacy ("buqi") of C. Radix.
28991204	8	47	theme	Radix	1285:1289	arg1	efficacy					1261:1268	healing efficacy	1253:1268	the healing efficacy ("buqi") of C. Radix	1249:1289	Five of the up-regulated compounds have been reported to show immune activity, which might contribute to the healing efficacy ("buqi") of C. Radix.
28991204	9	48	theme	main	1420:1423	arg1	compounds					1435:1443	some main bioactive compounds	1415:1443	some main bioactive compounds	1415:1443	The results of this study showed that treatment of C. Radix with PGR during cultivation has economic benefits and affected some main bioactive compounds in C. Radix.
28991204	9	49	theme	Radix	1346:1350	arg1	treatment					1330:1338	treatment	1330:1338	treatment of C. Radix with PGR during cultivation	1330:1378	The results of this study showed that treatment of C. Radix with PGR during cultivation has economic benefits and affected some main bioactive compounds in C. Radix.
28991204	4	50	theme	Radix	738:742	arg1	composition					697:707	the composition	693:707	the composition of untreated C. Radix and C. Radix treated with PGR	693:759	In the present study, amino acid analyses, targeted and non-targeted analyses by ultra-performance liquid chromatography combined with time-of-flight mass spectrometry (UPLC-TOF-MS) and gas chromatography-MS were used to analyze and compare the composition of untreated C. Radix and C. Radix treated with PGR.
28991204	9	51	with	treatment	1330:1338	arg1	PGR					1357:1359	PGR	1357:1359	PGR during cultivation	1357:1378	The results of this study showed that treatment of C. Radix with PGR during cultivation has economic benefits and affected some main bioactive compounds in C. Radix.
28991204	8	52	theme	healing	1253:1259	arg1	"					1276:1276	"buqi"	1271:1276	"buqi"	1271:1276	Five of the up-regulated compounds have been reported to show immune activity, which might contribute to the healing efficacy ("buqi") of C. Radix.
28991204	8	52	theme	healing	1253:1259	arg1	efficacy					1261:1268	healing efficacy	1253:1268	the healing efficacy ("buqi") of C. Radix	1249:1289	Five of the up-regulated compounds have been reported to show immune activity, which might contribute to the healing efficacy ("buqi") of C. Radix.
28991204	1	53	theme	cells	190:194	arg1	division					143:150	the cell division	134:150	the cell division of plant stem tip sub-apical meristem cells or primordial meristem cell	134:222	Plant growth retardant (PGR) refers to organics that can inhibit the cell division of plant stem tip sub-apical meristem cells or primordial meristem cell.
28991204	4	54	theme	Radix	725:729	arg1	composition					697:707	the composition	693:707	the composition of untreated C. Radix and C. Radix treated with PGR	693:759	In the present study, amino acid analyses, targeted and non-targeted analyses by ultra-performance liquid chromatography combined with time-of-flight mass spectrometry (UPLC-TOF-MS) and gas chromatography-MS were used to analyze and compare the composition of untreated C. Radix and C. Radix treated with PGR.
28991204	2	55	theme	plants	291:296	arg1	cultivation					253:263	the cultivation	249:263	the cultivation of rhizomatous functional plants; such as Codonopsis Radix, that is a famous Chinese traditional herb	249:365	They are widely used in the cultivation of rhizomatous functional plants; such as Codonopsis Radix, that is a famous Chinese traditional herb.
28991204	6	56	theme	PGR	970:972	arg1	treatment					974:982	PGR treatment	970:982	PGR treatment	970:982	The amounts of polysaccharides and some internal volatiles were significantly decreased by PGR treatment; while the free amino acids content was generally increased.
28991204	0	57	theme	Radix	62:66	arg1	Quality					40:46	Quality	40:46	Quality of Codonopsis Radix	40:66	Influence of Plant Growth Retardants on Quality of Codonopsis Radix.
28991204	2	58	theme	traditional	350:360	arg1	herb					362:365	a famous Chinese traditional herb	333:365	a famous Chinese traditional herb	333:365	They are widely used in the cultivation of rhizomatous functional plants; such as Codonopsis Radix, that is a famous Chinese traditional herb.
28991204	2	58	theme	traditional	350:360	arg1	Radix					318:322	Codonopsis Radix	307:322	Codonopsis Radix	307:322	They are widely used in the cultivation of rhizomatous functional plants; such as Codonopsis Radix, that is a famous Chinese traditional herb.
28991204	6	59	theme	free	995:998	arg1	acids					1006:1010	the free amino acids	991:1010	the free amino acids content	991:1018	The amounts of polysaccharides and some internal volatiles were significantly decreased by PGR treatment; while the free amino acids content was generally increased.
28991204	4	60	theme	gas	638:640	arg1	chromatography-MS					642:658	gas chromatography-MS	638:658	gas chromatography-MS	638:658	In the present study, amino acid analyses, targeted and non-targeted analyses by ultra-performance liquid chromatography combined with time-of-flight mass spectrometry (UPLC-TOF-MS) and gas chromatography-MS were used to analyze and compare the composition of untreated C. Radix and C. Radix treated with PGR.
28991204	1	61	theme	primordial	199:208	arg1	cell					219:222	primordial meristem cell	199:222	primordial meristem cell	199:222	Plant growth retardant (PGR) refers to organics that can inhibit the cell division of plant stem tip sub-apical meristem cells or primordial meristem cell.
28991204	0	62	theme	Codonopsis	51:60	arg1	Radix					62:66	Codonopsis Radix	51:66	Codonopsis Radix	51:66	Influence of Plant Growth Retardants on Quality of Codonopsis Radix.
28991204	4	63	theme	liquid	551:556	arg1	chromatography					558:571	ultra-performance liquid chromatography	533:571	ultra-performance liquid chromatography combined with time-of-flight mass spectrometry (UPLC-TOF-MS)	533:632	In the present study, amino acid analyses, targeted and non-targeted analyses by ultra-performance liquid chromatography combined with time-of-flight mass spectrometry (UPLC-TOF-MS) and gas chromatography-MS were used to analyze and compare the composition of untreated C. Radix and C. Radix treated with PGR.
28991204	4	64	theme	ultra-performance	533:549	arg1	chromatography					558:571	ultra-performance liquid chromatography	533:571	ultra-performance liquid chromatography combined with time-of-flight mass spectrometry (UPLC-TOF-MS)	533:632	In the present study, amino acid analyses, targeted and non-targeted analyses by ultra-performance liquid chromatography combined with time-of-flight mass spectrometry (UPLC-TOF-MS) and gas chromatography-MS were used to analyze and compare the composition of untreated C. Radix and C. Radix treated with PGR.
28991204	1	65	theme	meristem	210:217	arg1	cell					219:222	primordial meristem cell	199:222	primordial meristem cell	199:222	Plant growth retardant (PGR) refers to organics that can inhibit the cell division of plant stem tip sub-apical meristem cells or primordial meristem cell.
27465989	3	0	theme	different	552:560	arg1	starches					571:578	different modified starches	552:578	different modified starches	552:578	The EO microencapsulation efficiency in different modified starches, the retention of volatile aroma compounds, the emulsion particle size and the microstructure of the spray-dried encapsulated powdered products, as well as the compositional aroma changes taking place during the processing and storage for up to 8 months have been estimated.
27465989	5	1	theme	compositional	1146:1158	arg1	changes					1160:1166	the compositional changes	1142:1166	the compositional changes of the microencapsulated caraway EO constituents	1142:1215	However, the compositional changes of the microencapsulated caraway EO constituents were not remarkable.
27465989	5	1	theme	compositional	1146:1158	arg1	remarkable					1226:1235	remarkable	1226:1235	remarkable	1226:1235	However, the compositional changes of the microencapsulated caraway EO constituents were not remarkable.
27465989	4	2	theme	content	875:881	arg1	increase					859:866	The increase	855:866	The increase of OSA content from 0.97 to 2.52% in the P-native structure and from 0.91% to 2.66% in P-HP	855:958	The increase of OSA content from 0.97 to 2.52% in the P-native structure and from 0.91% to 2.66% in P-HP resulted in the significant increase in the encapsulating capacity, evaluated as a percentage of the total EO retained, from 61.6 to 88.0% and 73.8% to 84.0%, respectively.
27465989	6	3	theme	commercial	1338:1347	arg1	oil					1358:1360	rapeseed oil	1349:1360	rapeseed oil	1349:1360	Additionally, the effect of pure and encapsulated caraway EO products on the oxidative stability of commercial rapeseed oil and mayonnaise was evaluated using the instrumental Oxipres method and it was shown that they were more effective in emulsion type products by the up to 1.8-fold increase in the oxidative stability of mayonnaise.
27465989	3	4	theme	changes	760:766	arg1	retention					585:593	the retention	581:593	the retention of volatile aroma compounds	581:621	The EO microencapsulation efficiency in different modified starches, the retention of volatile aroma compounds, the emulsion particle size and the microstructure of the spray-dried encapsulated powdered products, as well as the compositional aroma changes taking place during the processing and storage for up to 8 months have been estimated.
27465989	3	4	theme	changes	760:766	arg1	size					646:649	the emulsion particle size	624:649	the emulsion particle size	624:649	The EO microencapsulation efficiency in different modified starches, the retention of volatile aroma compounds, the emulsion particle size and the microstructure of the spray-dried encapsulated powdered products, as well as the compositional aroma changes taking place during the processing and storage for up to 8 months have been estimated.
27465989	3	4	theme	changes	760:766	arg1	efficiency					538:547	The EO microencapsulation efficiency	512:547	The EO microencapsulation efficiency in different modified starches	512:578	The EO microencapsulation efficiency in different modified starches, the retention of volatile aroma compounds, the emulsion particle size and the microstructure of the spray-dried encapsulated powdered products, as well as the compositional aroma changes taking place during the processing and storage for up to 8 months have been estimated.
27465989	3	4	theme	changes	760:766	arg1	microstructure					659:672	the microstructure	655:672	the microstructure of the spray-dried encapsulated powdered products, as well as the compositional aroma changes taking place during the processing	655:801	The EO microencapsulation efficiency in different modified starches, the retention of volatile aroma compounds, the emulsion particle size and the microstructure of the spray-dried encapsulated powdered products, as well as the compositional aroma changes taking place during the processing and storage for up to 8 months have been estimated.
27465989	3	4	theme	changes	760:766	arg1	storage					807:813	storage	807:813	storage	807:813	The EO microencapsulation efficiency in different modified starches, the retention of volatile aroma compounds, the emulsion particle size and the microstructure of the spray-dried encapsulated powdered products, as well as the compositional aroma changes taking place during the processing and storage for up to 8 months have been estimated.
27465989	1	5	theme	propylene	194:202	arg1	oxide					204:208	propylene oxide	194:208	propylene oxide followed by esterification with octenyl succinic anhydride (OSA)	194:273	Hydroxypropylation with propylene oxide followed by esterification with octenyl succinic anhydride (OSA) was used to produce modified potato starch derivatives suitable for the encapsulation of essential oils.
27465989	6	6	theme	pure	1266:1269	arg1	products					1299:1306	pure and encapsulated caraway EO products	1266:1306	pure and encapsulated caraway EO products	1266:1306	Additionally, the effect of pure and encapsulated caraway EO products on the oxidative stability of commercial rapeseed oil and mayonnaise was evaluated using the instrumental Oxipres method and it was shown that they were more effective in emulsion type products by the up to 1.8-fold increase in the oxidative stability of mayonnaise.
27465989	6	7	theme	emulsion	1479:1486	arg1	products					1493:1500	emulsion type products	1479:1500	emulsion type products	1479:1500	Additionally, the effect of pure and encapsulated caraway EO products on the oxidative stability of commercial rapeseed oil and mayonnaise was evaluated using the instrumental Oxipres method and it was shown that they were more effective in emulsion type products by the up to 1.8-fold increase in the oxidative stability of mayonnaise.
27465989	4	8	theme	0.91	937:940	arg1	%					941:941	%	941:941	%	941:941	The increase of OSA content from 0.97 to 2.52% in the P-native structure and from 0.91% to 2.66% in P-HP resulted in the significant increase in the encapsulating capacity, evaluated as a percentage of the total EO retained, from 61.6 to 88.0% and 73.8% to 84.0%, respectively.
27465989	6	9	theme	EO	1296:1297	arg1	products					1299:1306	pure and encapsulated caraway EO products	1266:1306	pure and encapsulated caraway EO products	1266:1306	Additionally, the effect of pure and encapsulated caraway EO products on the oxidative stability of commercial rapeseed oil and mayonnaise was evaluated using the instrumental Oxipres method and it was shown that they were more effective in emulsion type products by the up to 1.8-fold increase in the oxidative stability of mayonnaise.
27465989	3	10	theme	aroma	607:611	arg1	compounds					613:621	volatile aroma compounds	598:621	volatile aroma compounds	598:621	The EO microencapsulation efficiency in different modified starches, the retention of volatile aroma compounds, the emulsion particle size and the microstructure of the spray-dried encapsulated powdered products, as well as the compositional aroma changes taking place during the processing and storage for up to 8 months have been estimated.
27465989	6	11	theme	encapsulated	1275:1286	arg1	products					1299:1306	pure and encapsulated caraway EO products	1266:1306	pure and encapsulated caraway EO products	1266:1306	Additionally, the effect of pure and encapsulated caraway EO products on the oxidative stability of commercial rapeseed oil and mayonnaise was evaluated using the instrumental Oxipres method and it was shown that they were more effective in emulsion type products by the up to 1.8-fold increase in the oxidative stability of mayonnaise.
27465989	1	12	theme	octenyl	242:248	arg1	OSA					270:272	OSA	270:272	OSA	270:272	Hydroxypropylation with propylene oxide followed by esterification with octenyl succinic anhydride (OSA) was used to produce modified potato starch derivatives suitable for the encapsulation of essential oils.
27465989	1	12	theme	octenyl	242:248	arg1	anhydride					259:267	octenyl succinic anhydride	242:267	octenyl succinic anhydride (OSA)	242:273	Hydroxypropylation with propylene oxide followed by esterification with octenyl succinic anhydride (OSA) was used to produce modified potato starch derivatives suitable for the encapsulation of essential oils.
27465989	4	13	theme	P-native	909:916	arg1	structure					918:926	the P-native structure	905:926	the P-native structure	905:926	The increase of OSA content from 0.97 to 2.52% in the P-native structure and from 0.91% to 2.66% in P-HP resulted in the significant increase in the encapsulating capacity, evaluated as a percentage of the total EO retained, from 61.6 to 88.0% and 73.8% to 84.0%, respectively.
27465989	0	14	theme	essential	154:162	arg1	oils					164:167	essential oils	154:167	essential oils	154:167	Preparation and characterization of single and dual propylene oxide and octenyl succinic anhydride modified starch carriers for the microencapsulation of essential oils.
27465989	3	15	theme	emulsion	628:635	arg1	size					646:649	the emulsion particle size	624:649	the emulsion particle size	624:649	The EO microencapsulation efficiency in different modified starches, the retention of volatile aroma compounds, the emulsion particle size and the microstructure of the spray-dried encapsulated powdered products, as well as the compositional aroma changes taking place during the processing and storage for up to 8 months have been estimated.
27465989	4	16	dep	resulted	960:967	arg1	evaluated					1028:1036	evaluated	1028:1036	evaluated as a percentage of the total EO retained, from 61.6 to 88.0% and 73.8% to 84.0%, respectively	1028:1130	The increase of OSA content from 0.97 to 2.52% in the P-native structure and from 0.91% to 2.66% in P-HP resulted in the significant increase in the encapsulating capacity, evaluated as a percentage of the total EO retained, from 61.6 to 88.0% and 73.8% to 84.0%, respectively.
27465989	0	17	theme	oxide	62:66	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of single and dual propylene oxide and octenyl succinic anhydride modified starch carriers for the microencapsulation of essential oils.
27465989	0	17	theme	oxide	62:66	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of single and dual propylene oxide and octenyl succinic anhydride modified starch carriers for the microencapsulation of essential oils.
27465989	4	18	theme	significant	976:986	arg1	increase					988:995	the significant increase	972:995	the significant increase in the encapsulating capacity	972:1025	The increase of OSA content from 0.97 to 2.52% in the P-native structure and from 0.91% to 2.66% in P-HP resulted in the significant increase in the encapsulating capacity, evaluated as a percentage of the total EO retained, from 61.6 to 88.0% and 73.8% to 84.0%, respectively.
27465989	0	19	theme	octenyl	72:78	arg1	anhydride					89:97	octenyl succinic anhydride	72:97	octenyl succinic anhydride	72:97	Preparation and characterization of single and dual propylene oxide and octenyl succinic anhydride modified starch carriers for the microencapsulation of essential oils.
27465989	1	20	theme	essential	364:372	arg1	oils					374:377	essential oils	364:377	essential oils	364:377	Hydroxypropylation with propylene oxide followed by esterification with octenyl succinic anhydride (OSA) was used to produce modified potato starch derivatives suitable for the encapsulation of essential oils.
27465989	1	21	with	Hydroxypropylation	170:187	arg1	oxide					204:208	propylene oxide	194:208	propylene oxide followed by esterification with octenyl succinic anhydride (OSA)	194:273	Hydroxypropylation with propylene oxide followed by esterification with octenyl succinic anhydride (OSA) was used to produce modified potato starch derivatives suitable for the encapsulation of essential oils.
27465989	4	22	dep	%	941:941	arg1	to					943:944	to	943:944	to	943:944	The increase of OSA content from 0.97 to 2.52% in the P-native structure and from 0.91% to 2.66% in P-HP resulted in the significant increase in the encapsulating capacity, evaluated as a percentage of the total EO retained, from 61.6 to 88.0% and 73.8% to 84.0%, respectively.
27465989	0	23	theme	propylene	52:60	arg1	oxide					62:66	single and dual propylene oxide	36:66	single and dual propylene oxide	36:66	Preparation and characterization of single and dual propylene oxide and octenyl succinic anhydride modified starch carriers for the microencapsulation of essential oils.
27465989	0	24	theme	starch	108:113	arg1	carriers					115:122	starch carriers	108:122	starch carriers for the microencapsulation of essential oils	108:167	Preparation and characterization of single and dual propylene oxide and octenyl succinic anhydride modified starch carriers for the microencapsulation of essential oils.
27465989	2	25	theme	modified	482:489	arg1	starches					502:509	enzymatically hydrolyzed dual/single modified and native starches	445:509	enzymatically hydrolyzed dual/single modified and native starches	445:509	Caraway essential oil (EO) was encapsulated by spray-drying into enzymatically hydrolyzed dual/single modified and native starches.
27465989	6	26	theme	instrumental	1401:1412	arg1	method					1422:1427	the instrumental Oxipres method	1397:1427	the instrumental Oxipres method	1397:1427	Additionally, the effect of pure and encapsulated caraway EO products on the oxidative stability of commercial rapeseed oil and mayonnaise was evaluated using the instrumental Oxipres method and it was shown that they were more effective in emulsion type products by the up to 1.8-fold increase in the oxidative stability of mayonnaise.
27465989	6	27	theme	mayonnaise	1563:1572	arg1	stability					1550:1558	the oxidative stability	1536:1558	the oxidative stability of mayonnaise	1536:1572	Additionally, the effect of pure and encapsulated caraway EO products on the oxidative stability of commercial rapeseed oil and mayonnaise was evaluated using the instrumental Oxipres method and it was shown that they were more effective in emulsion type products by the up to 1.8-fold increase in the oxidative stability of mayonnaise.
27465989	2	28	theme	hydrolyzed	459:468	arg1	starches					502:509	enzymatically hydrolyzed dual/single modified and native starches	445:509	enzymatically hydrolyzed dual/single modified and native starches	445:509	Caraway essential oil (EO) was encapsulated by spray-drying into enzymatically hydrolyzed dual/single modified and native starches.
27465989	1	29	theme	potato	304:309	arg1	derivatives					318:328	modified potato starch derivatives	295:328	modified potato starch derivatives suitable for the encapsulation of essential oils	295:377	Hydroxypropylation with propylene oxide followed by esterification with octenyl succinic anhydride (OSA) was used to produce modified potato starch derivatives suitable for the encapsulation of essential oils.
27465989	3	30	theme	powdered	706:713	arg1	products					715:722	the spray-dried encapsulated powdered products	677:722	the spray-dried encapsulated powdered products	677:722	The EO microencapsulation efficiency in different modified starches, the retention of volatile aroma compounds, the emulsion particle size and the microstructure of the spray-dried encapsulated powdered products, as well as the compositional aroma changes taking place during the processing and storage for up to 8 months have been estimated.
27465989	3	31	from	size	646:649	arg1	starches					571:578	different modified starches	552:578	different modified starches	552:578	The EO microencapsulation efficiency in different modified starches, the retention of volatile aroma compounds, the emulsion particle size and the microstructure of the spray-dried encapsulated powdered products, as well as the compositional aroma changes taking place during the processing and storage for up to 8 months have been estimated.
27465989	0	32	theme	single	36:41	arg1	oxide					62:66	single and dual propylene oxide	36:66	single and dual propylene oxide	36:66	Preparation and characterization of single and dual propylene oxide and octenyl succinic anhydride modified starch carriers for the microencapsulation of essential oils.
27465989	1	33	with	esterification	222:235	arg1	OSA					270:272	OSA	270:272	OSA	270:272	Hydroxypropylation with propylene oxide followed by esterification with octenyl succinic anhydride (OSA) was used to produce modified potato starch derivatives suitable for the encapsulation of essential oils.
27465989	1	33	with	esterification	222:235	arg1	anhydride					259:267	octenyl succinic anhydride	242:267	octenyl succinic anhydride (OSA)	242:273	Hydroxypropylation with propylene oxide followed by esterification with octenyl succinic anhydride (OSA) was used to produce modified potato starch derivatives suitable for the encapsulation of essential oils.
27465989	4	34	theme	encapsulating	1004:1016	arg1	capacity					1018:1025	the encapsulating capacity	1000:1025	the encapsulating capacity	1000:1025	The increase of OSA content from 0.97 to 2.52% in the P-native structure and from 0.91% to 2.66% in P-HP resulted in the significant increase in the encapsulating capacity, evaluated as a percentage of the total EO retained, from 61.6 to 88.0% and 73.8% to 84.0%, respectively.
27465989	3	35	theme	spray-dried	681:691	arg1	products					715:722	the spray-dried encapsulated powdered products	677:722	the spray-dried encapsulated powdered products	677:722	The EO microencapsulation efficiency in different modified starches, the retention of volatile aroma compounds, the emulsion particle size and the microstructure of the spray-dried encapsulated powdered products, as well as the compositional aroma changes taking place during the processing and storage for up to 8 months have been estimated.
27465989	3	36	theme	microencapsulation	519:536	arg1	efficiency					538:547	The EO microencapsulation efficiency	512:547	The EO microencapsulation efficiency in different modified starches	512:578	The EO microencapsulation efficiency in different modified starches, the retention of volatile aroma compounds, the emulsion particle size and the microstructure of the spray-dried encapsulated powdered products, as well as the compositional aroma changes taking place during the processing and storage for up to 8 months have been estimated.
27465989	0	37	theme	dual	47:50	arg1	oxide					62:66	single and dual propylene oxide	36:66	single and dual propylene oxide	36:66	Preparation and characterization of single and dual propylene oxide and octenyl succinic anhydride modified starch carriers for the microencapsulation of essential oils.
27465989	4	38	theme	EO	1067:1068	arg1	percentage					1043:1052	a percentage	1041:1052	a percentage of the total EO retained, from 61.6 to 88.0% and 73.8% to 84.0%, respectively	1041:1130	The increase of OSA content from 0.97 to 2.52% in the P-native structure and from 0.91% to 2.66% in P-HP resulted in the significant increase in the encapsulating capacity, evaluated as a percentage of the total EO retained, from 61.6 to 88.0% and 73.8% to 84.0%, respectively.
27465989	1	39	theme	succinic	250:257	arg1	OSA					270:272	OSA	270:272	OSA	270:272	Hydroxypropylation with propylene oxide followed by esterification with octenyl succinic anhydride (OSA) was used to produce modified potato starch derivatives suitable for the encapsulation of essential oils.
27465989	1	39	theme	succinic	250:257	arg1	anhydride					259:267	octenyl succinic anhydride	242:267	octenyl succinic anhydride (OSA)	242:273	Hydroxypropylation with propylene oxide followed by esterification with octenyl succinic anhydride (OSA) was used to produce modified potato starch derivatives suitable for the encapsulation of essential oils.
27465989	3	40	theme	modified	562:569	arg1	starches					571:578	different modified starches	552:578	different modified starches	552:578	The EO microencapsulation efficiency in different modified starches, the retention of volatile aroma compounds, the emulsion particle size and the microstructure of the spray-dried encapsulated powdered products, as well as the compositional aroma changes taking place during the processing and storage for up to 8 months have been estimated.
27465989	5	41	theme	caraway	1193:1199	arg1	constituents					1204:1215	the microencapsulated caraway EO constituents	1171:1215	the microencapsulated caraway EO constituents	1171:1215	However, the compositional changes of the microencapsulated caraway EO constituents were not remarkable.
27465989	2	42	theme	native	495:500	arg1	starches					502:509	enzymatically hydrolyzed dual/single modified and native starches	445:509	enzymatically hydrolyzed dual/single modified and native starches	445:509	Caraway essential oil (EO) was encapsulated by spray-drying into enzymatically hydrolyzed dual/single modified and native starches.
27465989	4	43	dep	2.52	896:899	arg1	to					893:894	to	893:894	to	893:894	The increase of OSA content from 0.97 to 2.52% in the P-native structure and from 0.91% to 2.66% in P-HP resulted in the significant increase in the encapsulating capacity, evaluated as a percentage of the total EO retained, from 61.6 to 88.0% and 73.8% to 84.0%, respectively.
27465989	6	44	from	effect	1256:1261	arg1	stability					1325:1333	the oxidative stability	1311:1333	the oxidative stability of commercial rapeseed oil and mayonnaise	1311:1375	Additionally, the effect of pure and encapsulated caraway EO products on the oxidative stability of commercial rapeseed oil and mayonnaise was evaluated using the instrumental Oxipres method and it was shown that they were more effective in emulsion type products by the up to 1.8-fold increase in the oxidative stability of mayonnaise.
27465989	3	45	theme	aroma	754:758	arg1	changes					760:766	the compositional aroma changes	736:766	the compositional aroma changes taking place during the processing	736:801	The EO microencapsulation efficiency in different modified starches, the retention of volatile aroma compounds, the emulsion particle size and the microstructure of the spray-dried encapsulated powdered products, as well as the compositional aroma changes taking place during the processing and storage for up to 8 months have been estimated.
27465989	2	46	theme	Caraway	380:386	arg1	EO					403:404	EO	403:404	EO	403:404	Caraway essential oil (EO) was encapsulated by spray-drying into enzymatically hydrolyzed dual/single modified and native starches.
27465989	2	46	theme	Caraway	380:386	arg1	oil					398:400	Caraway essential oil	380:400	Caraway essential oil (EO)	380:405	Caraway essential oil (EO) was encapsulated by spray-drying into enzymatically hydrolyzed dual/single modified and native starches.
27465989	4	47	from	%	950:950	arg1	P-HP					955:958	P-HP	955:958	P-HP	955:958	The increase of OSA content from 0.97 to 2.52% in the P-native structure and from 0.91% to 2.66% in P-HP resulted in the significant increase in the encapsulating capacity, evaluated as a percentage of the total EO retained, from 61.6 to 88.0% and 73.8% to 84.0%, respectively.
27465989	4	47	from	%	950:950	arg1	increase					859:866	The increase	855:866	The increase of OSA content from 0.97 to 2.52% in the P-native structure and from 0.91% to 2.66% in P-HP	855:958	The increase of OSA content from 0.97 to 2.52% in the P-native structure and from 0.91% to 2.66% in P-HP resulted in the significant increase in the encapsulating capacity, evaluated as a percentage of the total EO retained, from 61.6 to 88.0% and 73.8% to 84.0%, respectively.
27465989	4	48	dep	%	1116:1116	arg1	to					1109:1110	to	1109:1110	to	1109:1110	The increase of OSA content from 0.97 to 2.52% in the P-native structure and from 0.91% to 2.66% in P-HP resulted in the significant increase in the encapsulating capacity, evaluated as a percentage of the total EO retained, from 61.6 to 88.0% and 73.8% to 84.0%, respectively.
27465989	4	49	theme	OSA	871:873	arg1	content					875:881	OSA content	871:881	OSA content	871:881	The increase of OSA content from 0.97 to 2.52% in the P-native structure and from 0.91% to 2.66% in P-HP resulted in the significant increase in the encapsulating capacity, evaluated as a percentage of the total EO retained, from 61.6 to 88.0% and 73.8% to 84.0%, respectively.
27465989	4	50	theme	%	1107:1107	arg1	%					1116:1116	73.8% to 84.0%	1103:1116	73.8% to 84.0%	1103:1116	The increase of OSA content from 0.97 to 2.52% in the P-native structure and from 0.91% to 2.66% in P-HP resulted in the significant increase in the encapsulating capacity, evaluated as a percentage of the total EO retained, from 61.6 to 88.0% and 73.8% to 84.0%, respectively.
27465989	3	51	theme	compounds	613:621	arg1	retention					585:593	the retention	581:593	the retention of volatile aroma compounds	581:621	The EO microencapsulation efficiency in different modified starches, the retention of volatile aroma compounds, the emulsion particle size and the microstructure of the spray-dried encapsulated powdered products, as well as the compositional aroma changes taking place during the processing and storage for up to 8 months have been estimated.
27465989	3	51	theme	compounds	613:621	arg1	size					646:649	the emulsion particle size	624:649	the emulsion particle size	624:649	The EO microencapsulation efficiency in different modified starches, the retention of volatile aroma compounds, the emulsion particle size and the microstructure of the spray-dried encapsulated powdered products, as well as the compositional aroma changes taking place during the processing and storage for up to 8 months have been estimated.
27465989	3	51	theme	compounds	613:621	arg1	efficiency					538:547	The EO microencapsulation efficiency	512:547	The EO microencapsulation efficiency in different modified starches	512:578	The EO microencapsulation efficiency in different modified starches, the retention of volatile aroma compounds, the emulsion particle size and the microstructure of the spray-dried encapsulated powdered products, as well as the compositional aroma changes taking place during the processing and storage for up to 8 months have been estimated.
27465989	3	51	theme	compounds	613:621	arg1	microstructure					659:672	the microstructure	655:672	the microstructure of the spray-dried encapsulated powdered products, as well as the compositional aroma changes taking place during the processing	655:801	The EO microencapsulation efficiency in different modified starches, the retention of volatile aroma compounds, the emulsion particle size and the microstructure of the spray-dried encapsulated powdered products, as well as the compositional aroma changes taking place during the processing and storage for up to 8 months have been estimated.
27465989	3	51	theme	compounds	613:621	arg1	storage					807:813	storage	807:813	storage	807:813	The EO microencapsulation efficiency in different modified starches, the retention of volatile aroma compounds, the emulsion particle size and the microstructure of the spray-dried encapsulated powdered products, as well as the compositional aroma changes taking place during the processing and storage for up to 8 months have been estimated.
27465989	6	52	theme	type	1488:1491	arg1	products					1493:1500	emulsion type products	1479:1500	emulsion type products	1479:1500	Additionally, the effect of pure and encapsulated caraway EO products on the oxidative stability of commercial rapeseed oil and mayonnaise was evaluated using the instrumental Oxipres method and it was shown that they were more effective in emulsion type products by the up to 1.8-fold increase in the oxidative stability of mayonnaise.
27465989	6	53	theme	products	1299:1306	arg1	effect					1256:1261	the effect	1252:1261	the effect of pure and encapsulated caraway EO products on the oxidative stability of commercial rapeseed oil and mayonnaise	1252:1375	Additionally, the effect of pure and encapsulated caraway EO products on the oxidative stability of commercial rapeseed oil and mayonnaise was evaluated using the instrumental Oxipres method and it was shown that they were more effective in emulsion type products by the up to 1.8-fold increase in the oxidative stability of mayonnaise.
27465989	3	54	from	efficiency	538:547	arg1	starches					571:578	different modified starches	552:578	different modified starches	552:578	The EO microencapsulation efficiency in different modified starches, the retention of volatile aroma compounds, the emulsion particle size and the microstructure of the spray-dried encapsulated powdered products, as well as the compositional aroma changes taking place during the processing and storage for up to 8 months have been estimated.
27465989	3	55	theme	volatile	598:605	arg1	compounds					613:621	volatile aroma compounds	598:621	volatile aroma compounds	598:621	The EO microencapsulation efficiency in different modified starches, the retention of volatile aroma compounds, the emulsion particle size and the microstructure of the spray-dried encapsulated powdered products, as well as the compositional aroma changes taking place during the processing and storage for up to 8 months have been estimated.
27465989	6	56	theme	caraway	1288:1294	arg1	products					1299:1306	pure and encapsulated caraway EO products	1266:1306	pure and encapsulated caraway EO products	1266:1306	Additionally, the effect of pure and encapsulated caraway EO products on the oxidative stability of commercial rapeseed oil and mayonnaise was evaluated using the instrumental Oxipres method and it was shown that they were more effective in emulsion type products by the up to 1.8-fold increase in the oxidative stability of mayonnaise.
27465989	3	57	theme	particle	637:644	arg1	size					646:649	the emulsion particle size	624:649	the emulsion particle size	624:649	The EO microencapsulation efficiency in different modified starches, the retention of volatile aroma compounds, the emulsion particle size and the microstructure of the spray-dried encapsulated powdered products, as well as the compositional aroma changes taking place during the processing and storage for up to 8 months have been estimated.
27465989	0	58	theme	oils	164:167	arg1	microencapsulation					132:149	the microencapsulation	128:149	the microencapsulation of essential oils	128:167	Preparation and characterization of single and dual propylene oxide and octenyl succinic anhydride modified starch carriers for the microencapsulation of essential oils.
27465989	6	59	theme	oxidative	1540:1548	arg1	stability					1550:1558	the oxidative stability	1536:1558	the oxidative stability of mayonnaise	1536:1572	Additionally, the effect of pure and encapsulated caraway EO products on the oxidative stability of commercial rapeseed oil and mayonnaise was evaluated using the instrumental Oxipres method and it was shown that they were more effective in emulsion type products by the up to 1.8-fold increase in the oxidative stability of mayonnaise.
27465989	6	60	theme	oil	1358:1360	arg1	stability					1325:1333	the oxidative stability	1311:1333	the oxidative stability of commercial rapeseed oil and mayonnaise	1311:1375	Additionally, the effect of pure and encapsulated caraway EO products on the oxidative stability of commercial rapeseed oil and mayonnaise was evaluated using the instrumental Oxipres method and it was shown that they were more effective in emulsion type products by the up to 1.8-fold increase in the oxidative stability of mayonnaise.
27465989	6	61	theme	1.8-fold	1515:1522	arg1	increase					1524:1531	the up to 1.8-fold increase	1505:1531	the up to 1.8-fold increase in the oxidative stability of mayonnaise	1505:1572	Additionally, the effect of pure and encapsulated caraway EO products on the oxidative stability of commercial rapeseed oil and mayonnaise was evaluated using the instrumental Oxipres method and it was shown that they were more effective in emulsion type products by the up to 1.8-fold increase in the oxidative stability of mayonnaise.
27465989	6	62	dep	1.8-fold	1515:1522	arg1	to					1512:1513	to	1512:1513	to	1512:1513	Additionally, the effect of pure and encapsulated caraway EO products on the oxidative stability of commercial rapeseed oil and mayonnaise was evaluated using the instrumental Oxipres method and it was shown that they were more effective in emulsion type products by the up to 1.8-fold increase in the oxidative stability of mayonnaise.
27465989	5	63	theme	constituents	1204:1215	arg1	changes					1160:1166	the compositional changes	1142:1166	the compositional changes of the microencapsulated caraway EO constituents	1142:1215	However, the compositional changes of the microencapsulated caraway EO constituents were not remarkable.
27465989	5	63	theme	constituents	1204:1215	arg1	remarkable					1226:1235	remarkable	1226:1235	remarkable	1226:1235	However, the compositional changes of the microencapsulated caraway EO constituents were not remarkable.
27465989	0	64	theme	anhydride	89:97	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of single and dual propylene oxide and octenyl succinic anhydride modified starch carriers for the microencapsulation of essential oils.
27465989	0	64	theme	anhydride	89:97	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of single and dual propylene oxide and octenyl succinic anhydride modified starch carriers for the microencapsulation of essential oils.
27465989	0	65	theme	succinic	80:87	arg1	anhydride					89:97	octenyl succinic anhydride	72:97	octenyl succinic anhydride	72:97	Preparation and characterization of single and dual propylene oxide and octenyl succinic anhydride modified starch carriers for the microencapsulation of essential oils.
27465989	4	66	from	%	900:900	arg1	structure					918:926	the P-native structure	905:926	the P-native structure	905:926	The increase of OSA content from 0.97 to 2.52% in the P-native structure and from 0.91% to 2.66% in P-HP resulted in the significant increase in the encapsulating capacity, evaluated as a percentage of the total EO retained, from 61.6 to 88.0% and 73.8% to 84.0%, respectively.
27465989	4	66	from	%	900:900	arg1	increase					859:866	The increase	855:866	The increase of OSA content from 0.97 to 2.52% in the P-native structure and from 0.91% to 2.66% in P-HP	855:958	The increase of OSA content from 0.97 to 2.52% in the P-native structure and from 0.91% to 2.66% in P-HP resulted in the significant increase in the encapsulating capacity, evaluated as a percentage of the total EO retained, from 61.6 to 88.0% and 73.8% to 84.0%, respectively.
27465989	4	67	dep	%	1097:1097	arg1	to					1090:1091	to	1090:1091	to	1090:1091	The increase of OSA content from 0.97 to 2.52% in the P-native structure and from 0.91% to 2.66% in P-HP resulted in the significant increase in the encapsulating capacity, evaluated as a percentage of the total EO retained, from 61.6 to 88.0% and 73.8% to 84.0%, respectively.
27465989	6	68	from	increase	1524:1531	arg1	stability					1550:1558	the oxidative stability	1536:1558	the oxidative stability of mayonnaise	1536:1572	Additionally, the effect of pure and encapsulated caraway EO products on the oxidative stability of commercial rapeseed oil and mayonnaise was evaluated using the instrumental Oxipres method and it was shown that they were more effective in emulsion type products by the up to 1.8-fold increase in the oxidative stability of mayonnaise.
27465989	6	69	theme	rapeseed	1349:1356	arg1	oil					1358:1360	rapeseed oil	1349:1360	rapeseed oil	1349:1360	Additionally, the effect of pure and encapsulated caraway EO products on the oxidative stability of commercial rapeseed oil and mayonnaise was evaluated using the instrumental Oxipres method and it was shown that they were more effective in emulsion type products by the up to 1.8-fold increase in the oxidative stability of mayonnaise.
27465989	3	70	from	retention	585:593	arg1	starches					571:578	different modified starches	552:578	different modified starches	552:578	The EO microencapsulation efficiency in different modified starches, the retention of volatile aroma compounds, the emulsion particle size and the microstructure of the spray-dried encapsulated powdered products, as well as the compositional aroma changes taking place during the processing and storage for up to 8 months have been estimated.
27465989	1	71	theme	oils	374:377	arg1	encapsulation					347:359	the encapsulation	343:359	the encapsulation of essential oils	343:377	Hydroxypropylation with propylene oxide followed by esterification with octenyl succinic anhydride (OSA) was used to produce modified potato starch derivatives suitable for the encapsulation of essential oils.
27465989	6	72	from	products	1493:1500	arg1	effective					1466:1474	effective	1466:1474	effective	1466:1474	Additionally, the effect of pure and encapsulated caraway EO products on the oxidative stability of commercial rapeseed oil and mayonnaise was evaluated using the instrumental Oxipres method and it was shown that they were more effective in emulsion type products by the up to 1.8-fold increase in the oxidative stability of mayonnaise.
27465989	4	73	theme	2.66	946:949	arg1	%					941:941	%	941:941	%	941:941	The increase of OSA content from 0.97 to 2.52% in the P-native structure and from 0.91% to 2.66% in P-HP resulted in the significant increase in the encapsulating capacity, evaluated as a percentage of the total EO retained, from 61.6 to 88.0% and 73.8% to 84.0%, respectively.
27465989	3	74	from	microstructure	659:672	arg1	starches					571:578	different modified starches	552:578	different modified starches	552:578	The EO microencapsulation efficiency in different modified starches, the retention of volatile aroma compounds, the emulsion particle size and the microstructure of the spray-dried encapsulated powdered products, as well as the compositional aroma changes taking place during the processing and storage for up to 8 months have been estimated.
27465989	6	75	theme	Oxipres	1414:1420	arg1	method					1422:1427	the instrumental Oxipres method	1397:1427	the instrumental Oxipres method	1397:1427	Additionally, the effect of pure and encapsulated caraway EO products on the oxidative stability of commercial rapeseed oil and mayonnaise was evaluated using the instrumental Oxipres method and it was shown that they were more effective in emulsion type products by the up to 1.8-fold increase in the oxidative stability of mayonnaise.
27465989	3	76	dep	8	825:825	arg1	to					822:823	to	822:823	to	822:823	The EO microencapsulation efficiency in different modified starches, the retention of volatile aroma compounds, the emulsion particle size and the microstructure of the spray-dried encapsulated powdered products, as well as the compositional aroma changes taking place during the processing and storage for up to 8 months have been estimated.
27465989	6	77	from	effective	1466:1474	arg1	products					1493:1500	emulsion type products	1479:1500	emulsion type products	1479:1500	Additionally, the effect of pure and encapsulated caraway EO products on the oxidative stability of commercial rapeseed oil and mayonnaise was evaluated using the instrumental Oxipres method and it was shown that they were more effective in emulsion type products by the up to 1.8-fold increase in the oxidative stability of mayonnaise.
27465989	3	78	from	storage	807:813	arg1	starches					571:578	different modified starches	552:578	different modified starches	552:578	The EO microencapsulation efficiency in different modified starches, the retention of volatile aroma compounds, the emulsion particle size and the microstructure of the spray-dried encapsulated powdered products, as well as the compositional aroma changes taking place during the processing and storage for up to 8 months have been estimated.
27465989	1	79	theme	modified	295:302	arg1	derivatives					318:328	modified potato starch derivatives	295:328	modified potato starch derivatives suitable for the encapsulation of essential oils	295:377	Hydroxypropylation with propylene oxide followed by esterification with octenyl succinic anhydride (OSA) was used to produce modified potato starch derivatives suitable for the encapsulation of essential oils.
27465989	3	80	theme	encapsulated	693:704	arg1	products					715:722	the spray-dried encapsulated powdered products	677:722	the spray-dried encapsulated powdered products	677:722	The EO microencapsulation efficiency in different modified starches, the retention of volatile aroma compounds, the emulsion particle size and the microstructure of the spray-dried encapsulated powdered products, as well as the compositional aroma changes taking place during the processing and storage for up to 8 months have been estimated.
27465989	1	81	theme	starch	311:316	arg1	derivatives					318:328	modified potato starch derivatives	295:328	modified potato starch derivatives suitable for the encapsulation of essential oils	295:377	Hydroxypropylation with propylene oxide followed by esterification with octenyl succinic anhydride (OSA) was used to produce modified potato starch derivatives suitable for the encapsulation of essential oils.
27465989	1	82	used	used	279:282	arg2	Hydroxypropylation					170:187	Hydroxypropylation	170:187	Hydroxypropylation with propylene oxide followed by esterification with octenyl succinic anhydride (OSA)	170:273	Hydroxypropylation with propylene oxide followed by esterification with octenyl succinic anhydride (OSA) was used to produce modified potato starch derivatives suitable for the encapsulation of essential oils.
27465989	6	83	theme	mayonnaise	1366:1375	arg1	stability					1325:1333	the oxidative stability	1311:1333	the oxidative stability of commercial rapeseed oil and mayonnaise	1311:1375	Additionally, the effect of pure and encapsulated caraway EO products on the oxidative stability of commercial rapeseed oil and mayonnaise was evaluated using the instrumental Oxipres method and it was shown that they were more effective in emulsion type products by the up to 1.8-fold increase in the oxidative stability of mayonnaise.
27465989	1	84	theme	suitable	330:337	arg1	derivatives					318:328	modified potato starch derivatives	295:328	modified potato starch derivatives suitable for the encapsulation of essential oils	295:377	Hydroxypropylation with propylene oxide followed by esterification with octenyl succinic anhydride (OSA) was used to produce modified potato starch derivatives suitable for the encapsulation of essential oils.
27465989	4	85	from	increase	988:995	arg1	capacity					1018:1025	the encapsulating capacity	1000:1025	the encapsulating capacity	1000:1025	The increase of OSA content from 0.97 to 2.52% in the P-native structure and from 0.91% to 2.66% in P-HP resulted in the significant increase in the encapsulating capacity, evaluated as a percentage of the total EO retained, from 61.6 to 88.0% and 73.8% to 84.0%, respectively.
27465989	4	86	theme	total	1061:1065	arg1	EO					1067:1068	the total EO	1057:1068	the total EO retained, from 61.6 to 88.0% and 73.8% to 84.0%, respectively	1057:1130	The increase of OSA content from 0.97 to 2.52% in the P-native structure and from 0.91% to 2.66% in P-HP resulted in the significant increase in the encapsulating capacity, evaluated as a percentage of the total EO retained, from 61.6 to 88.0% and 73.8% to 84.0%, respectively.
27465989	3	87	theme	products	715:722	arg1	retention					585:593	the retention	581:593	the retention of volatile aroma compounds	581:621	The EO microencapsulation efficiency in different modified starches, the retention of volatile aroma compounds, the emulsion particle size and the microstructure of the spray-dried encapsulated powdered products, as well as the compositional aroma changes taking place during the processing and storage for up to 8 months have been estimated.
27465989	3	87	theme	products	715:722	arg1	size					646:649	the emulsion particle size	624:649	the emulsion particle size	624:649	The EO microencapsulation efficiency in different modified starches, the retention of volatile aroma compounds, the emulsion particle size and the microstructure of the spray-dried encapsulated powdered products, as well as the compositional aroma changes taking place during the processing and storage for up to 8 months have been estimated.
27465989	3	87	theme	products	715:722	arg1	efficiency					538:547	The EO microencapsulation efficiency	512:547	The EO microencapsulation efficiency in different modified starches	512:578	The EO microencapsulation efficiency in different modified starches, the retention of volatile aroma compounds, the emulsion particle size and the microstructure of the spray-dried encapsulated powdered products, as well as the compositional aroma changes taking place during the processing and storage for up to 8 months have been estimated.
27465989	3	87	theme	products	715:722	arg1	microstructure					659:672	the microstructure	655:672	the microstructure of the spray-dried encapsulated powdered products, as well as the compositional aroma changes taking place during the processing	655:801	The EO microencapsulation efficiency in different modified starches, the retention of volatile aroma compounds, the emulsion particle size and the microstructure of the spray-dried encapsulated powdered products, as well as the compositional aroma changes taking place during the processing and storage for up to 8 months have been estimated.
27465989	3	87	theme	products	715:722	arg1	storage					807:813	storage	807:813	storage	807:813	The EO microencapsulation efficiency in different modified starches, the retention of volatile aroma compounds, the emulsion particle size and the microstructure of the spray-dried encapsulated powdered products, as well as the compositional aroma changes taking place during the processing and storage for up to 8 months have been estimated.
27465989	3	88	theme	EO	516:517	arg1	efficiency					538:547	The EO microencapsulation efficiency	512:547	The EO microencapsulation efficiency in different modified starches	512:578	The EO microencapsulation efficiency in different modified starches, the retention of volatile aroma compounds, the emulsion particle size and the microstructure of the spray-dried encapsulated powdered products, as well as the compositional aroma changes taking place during the processing and storage for up to 8 months have been estimated.
27465989	6	89	theme	oxidative	1315:1323	arg1	stability					1325:1333	the oxidative stability	1311:1333	the oxidative stability of commercial rapeseed oil and mayonnaise	1311:1375	Additionally, the effect of pure and encapsulated caraway EO products on the oxidative stability of commercial rapeseed oil and mayonnaise was evaluated using the instrumental Oxipres method and it was shown that they were more effective in emulsion type products by the up to 1.8-fold increase in the oxidative stability of mayonnaise.
27465989	5	90	theme	microencapsulated	1175:1191	arg1	constituents					1204:1215	the microencapsulated caraway EO constituents	1171:1215	the microencapsulated caraway EO constituents	1171:1215	However, the compositional changes of the microencapsulated caraway EO constituents were not remarkable.
27465989	3	91	theme	compositional	740:752	arg1	changes					760:766	the compositional aroma changes	736:766	the compositional aroma changes taking place during the processing	736:801	The EO microencapsulation efficiency in different modified starches, the retention of volatile aroma compounds, the emulsion particle size and the microstructure of the spray-dried encapsulated powdered products, as well as the compositional aroma changes taking place during the processing and storage for up to 8 months have been estimated.
27465989	2	92	theme	essential	388:396	arg1	EO					403:404	EO	403:404	EO	403:404	Caraway essential oil (EO) was encapsulated by spray-drying into enzymatically hydrolyzed dual/single modified and native starches.
27465989	2	92	theme	essential	388:396	arg1	oil					398:400	Caraway essential oil	380:400	Caraway essential oil (EO)	380:405	Caraway essential oil (EO) was encapsulated by spray-drying into enzymatically hydrolyzed dual/single modified and native starches.
27465989	5	93	theme	EO	1201:1202	arg1	constituents					1204:1215	the microencapsulated caraway EO constituents	1171:1215	the microencapsulated caraway EO constituents	1171:1215	However, the compositional changes of the microencapsulated caraway EO constituents were not remarkable.
24436203	2	0	theme	dry	452:454	arg1	state					456:460	dry state	452:460	dry state	452:460	Therefore, the objective of the current study is to compare the effects of γ-irradiation (dry state, 20-80 kGy), partial (1 and 4%) periodate oxidation (aqueous solution), and autoclaving (dry state) on the molecular weight of alginate, as well as the degradation behavior of alginate-based composites.
24436203	2	0	theme	dry	452:454	arg1	autoclaving					439:449	autoclaving	439:449	autoclaving (dry state)	439:461	Therefore, the objective of the current study is to compare the effects of γ-irradiation (dry state, 20-80 kGy), partial (1 and 4%) periodate oxidation (aqueous solution), and autoclaving (dry state) on the molecular weight of alginate, as well as the degradation behavior of alginate-based composites.
24436203	5	1	theme	resulting	1151:1159	arg1	composites					1185:1194	the resulting alginate-hydroxyapatite composites	1147:1194	the resulting alginate-hydroxyapatite composites	1147:1194	Autoclaving is shown to be a powerful tool to reduce the molecular weight of alginate in a controllable and mild manner without compromising the integrity of the resulting alginate-hydroxyapatite composites, simply by increasing the number of repetitive autoclaving cycles.
24436203	2	2	theme	composites	554:563	arg1	behavior					527:534	the degradation behavior	511:534	the degradation behavior of alginate-based composites	511:563	Therefore, the objective of the current study is to compare the effects of γ-irradiation (dry state, 20-80 kGy), partial (1 and 4%) periodate oxidation (aqueous solution), and autoclaving (dry state) on the molecular weight of alginate, as well as the degradation behavior of alginate-based composites.
24436203	2	2	theme	composites	554:563	arg1	effects					327:333	the effects	323:333	the effects of γ-irradiation (dry state, 20-80 kGy), partial (1 and 4%) periodate oxidation (aqueous solution), and autoclaving (dry state) on the molecular weight of alginate	323:497	Therefore, the objective of the current study is to compare the effects of γ-irradiation (dry state, 20-80 kGy), partial (1 and 4%) periodate oxidation (aqueous solution), and autoclaving (dry state) on the molecular weight of alginate, as well as the degradation behavior of alginate-based composites.
24436203	4	3	theme	molecular	866:874	arg1	weight					876:881	molecular weight	866:881	molecular weight	866:881	Partial periodate oxidation is less destructive as characterized by more moderate decreases in molecular weight, but the production of hydrolytically labile bonds compromises the integrity of the resulting composites.
24436203	5	4	theme	composites	1185:1194	arg1	integrity					1134:1142	the integrity	1130:1142	the integrity of the resulting alginate-hydroxyapatite composites	1130:1194	Autoclaving is shown to be a powerful tool to reduce the molecular weight of alginate in a controllable and mild manner without compromising the integrity of the resulting alginate-hydroxyapatite composites, simply by increasing the number of repetitive autoclaving cycles.
24436203	5	5	theme	mild	1097:1100	arg1	manner					1102:1107	a controllable and mild manner	1078:1107	a controllable and mild manner	1078:1107	Autoclaving is shown to be a powerful tool to reduce the molecular weight of alginate in a controllable and mild manner without compromising the integrity of the resulting alginate-hydroxyapatite composites, simply by increasing the number of repetitive autoclaving cycles.
24436203	2	6	theme	alginate-based	539:552	arg1	composites					554:563	alginate-based composites	539:563	alginate-based composites	539:563	Therefore, the objective of the current study is to compare the effects of γ-irradiation (dry state, 20-80 kGy), partial (1 and 4%) periodate oxidation (aqueous solution), and autoclaving (dry state) on the molecular weight of alginate, as well as the degradation behavior of alginate-based composites.
24436203	5	7	theme	cycles	1255:1260	arg1	number					1222:1227	the number	1218:1227	the number of repetitive autoclaving cycles	1218:1260	Autoclaving is shown to be a powerful tool to reduce the molecular weight of alginate in a controllable and mild manner without compromising the integrity of the resulting alginate-hydroxyapatite composites, simply by increasing the number of repetitive autoclaving cycles.
24436203	0	8	theme	alginate-hydroxyapatite	87:109	arg1	composites					111:120	alginate-hydroxyapatite composites	87:120	alginate-hydroxyapatite composites	87:120	Effects of physical and chemical treatments on the molecular weight and degradation of alginate-hydroxyapatite composites.
24436203	0	9	from	Effects	0:6	arg1	degradation					72:82	degradation	72:82	degradation	72:82	Effects of physical and chemical treatments on the molecular weight and degradation of alginate-hydroxyapatite composites.
24436203	0	9	from	Effects	0:6	arg1	weight					61:66	molecular weight	51:66	molecular weight	51:66	Effects of physical and chemical treatments on the molecular weight and degradation of alginate-hydroxyapatite composites.
24436203	3	10	from	soaking	738:744	arg1	fluid					764:768	simulated body fluid	749:768	simulated body fluid	749:768	The results show that γ-irradiation is by far the most destructive technique characterized by strongly reduced molecular weights and rapid loss of composite integrity upon soaking in simulated body fluid.
24436203	2	11	from	effects	327:333	arg1	weight					480:485	the molecular weight	466:485	the molecular weight of alginate	466:497	Therefore, the objective of the current study is to compare the effects of γ-irradiation (dry state, 20-80 kGy), partial (1 and 4%) periodate oxidation (aqueous solution), and autoclaving (dry state) on the molecular weight of alginate, as well as the degradation behavior of alginate-based composites.
24436203	4	12	theme	bonds	928:932	arg1	production					892:901	the production	888:901	the production of hydrolytically labile bonds	888:932	Partial periodate oxidation is less destructive as characterized by more moderate decreases in molecular weight, but the production of hydrolytically labile bonds compromises the integrity of the resulting composites.
24436203	2	13	theme	molecular	470:478	arg1	weight					480:485	the molecular weight	466:485	the molecular weight of alginate	466:497	Therefore, the objective of the current study is to compare the effects of γ-irradiation (dry state, 20-80 kGy), partial (1 and 4%) periodate oxidation (aqueous solution), and autoclaving (dry state) on the molecular weight of alginate, as well as the degradation behavior of alginate-based composites.
24436203	4	14	theme	moderate	844:851	arg1	decreases					853:861	more moderate decreases	839:861	more moderate decreases in molecular weight	839:881	Partial periodate oxidation is less destructive as characterized by more moderate decreases in molecular weight, but the production of hydrolytically labile bonds compromises the integrity of the resulting composites.
24436203	5	15	theme	alginate	1066:1073	arg1	weight					1056:1061	the molecular weight	1042:1061	the molecular weight of alginate	1042:1073	Autoclaving is shown to be a powerful tool to reduce the molecular weight of alginate in a controllable and mild manner without compromising the integrity of the resulting alginate-hydroxyapatite composites, simply by increasing the number of repetitive autoclaving cycles.
24436203	5	16	theme	autoclaving	1243:1253	arg1	cycles					1255:1260	repetitive autoclaving cycles	1232:1260	repetitive autoclaving cycles	1232:1260	Autoclaving is shown to be a powerful tool to reduce the molecular weight of alginate in a controllable and mild manner without compromising the integrity of the resulting alginate-hydroxyapatite composites, simply by increasing the number of repetitive autoclaving cycles.
24436203	2	17	theme	study	303:307	arg1	objective					278:286	the objective	274:286	the objective of the current study	274:307	Therefore, the objective of the current study is to compare the effects of γ-irradiation (dry state, 20-80 kGy), partial (1 and 4%) periodate oxidation (aqueous solution), and autoclaving (dry state) on the molecular weight of alginate, as well as the degradation behavior of alginate-based composites.
24436203	0	18	theme	composites	111:120	arg1	degradation					72:82	degradation	72:82	degradation	72:82	Effects of physical and chemical treatments on the molecular weight and degradation of alginate-hydroxyapatite composites.
24436203	0	18	theme	composites	111:120	arg1	weight					61:66	molecular weight	51:66	molecular weight	51:66	Effects of physical and chemical treatments on the molecular weight and degradation of alginate-hydroxyapatite composites.
24436203	3	19	theme	reduced	669:675	arg1	weights					687:693	strongly reduced molecular weights	660:693	strongly reduced molecular weights	660:693	The results show that γ-irradiation is by far the most destructive technique characterized by strongly reduced molecular weights and rapid loss of composite integrity upon soaking in simulated body fluid.
24436203	0	20	theme	treatments	33:42	arg1	Effects					0:6	Effects	0:6	Effects of physical and chemical treatments on the molecular weight and degradation of alginate-hydroxyapatite composites	0:120	Effects of physical and chemical treatments on the molecular weight and degradation of alginate-hydroxyapatite composites.
24436203	2	21	theme	current	295:301	arg1	study					303:307	the current study	291:307	the current study	291:307	Therefore, the objective of the current study is to compare the effects of γ-irradiation (dry state, 20-80 kGy), partial (1 and 4%) periodate oxidation (aqueous solution), and autoclaving (dry state) on the molecular weight of alginate, as well as the degradation behavior of alginate-based composites.
24436203	1	22	theme	predictable	181:191	arg1	performance					204:214	predictable biological performance	181:214	predictable biological performance	181:214	Degradation of alginate remains a critical issue to allow predictable biological performance upon implantation of alginate-based materials.
24436203	3	23	theme	molecular	677:685	arg1	weights					687:693	strongly reduced molecular weights	660:693	strongly reduced molecular weights	660:693	The results show that γ-irradiation is by far the most destructive technique characterized by strongly reduced molecular weights and rapid loss of composite integrity upon soaking in simulated body fluid.
24436203	1	24	theme	biological	193:202	arg1	performance					204:214	predictable biological performance	181:214	predictable biological performance	181:214	Degradation of alginate remains a critical issue to allow predictable biological performance upon implantation of alginate-based materials.
24436203	2	25	theme	aqueous	416:422	arg1	oxidation					405:413	partial (1 and 4%) periodate oxidation	376:413	partial (1 and 4%) periodate oxidation (aqueous solution)	376:432	Therefore, the objective of the current study is to compare the effects of γ-irradiation (dry state, 20-80 kGy), partial (1 and 4%) periodate oxidation (aqueous solution), and autoclaving (dry state) on the molecular weight of alginate, as well as the degradation behavior of alginate-based composites.
24436203	2	25	theme	aqueous	416:422	arg1	solution					424:431	aqueous solution	416:431	aqueous solution	416:431	Therefore, the objective of the current study is to compare the effects of γ-irradiation (dry state, 20-80 kGy), partial (1 and 4%) periodate oxidation (aqueous solution), and autoclaving (dry state) on the molecular weight of alginate, as well as the degradation behavior of alginate-based composites.
24436203	2	26	theme	partial	376:382	arg1	oxidation					405:413	partial (1 and 4%) periodate oxidation	376:413	partial (1 and 4%) periodate oxidation (aqueous solution)	376:432	Therefore, the objective of the current study is to compare the effects of γ-irradiation (dry state, 20-80 kGy), partial (1 and 4%) periodate oxidation (aqueous solution), and autoclaving (dry state) on the molecular weight of alginate, as well as the degradation behavior of alginate-based composites.
24436203	2	26	theme	partial	376:382	arg1	solution					424:431	aqueous solution	416:431	aqueous solution	416:431	Therefore, the objective of the current study is to compare the effects of γ-irradiation (dry state, 20-80 kGy), partial (1 and 4%) periodate oxidation (aqueous solution), and autoclaving (dry state) on the molecular weight of alginate, as well as the degradation behavior of alginate-based composites.
24436203	2	27	from	behavior	527:534	arg1	weight					480:485	the molecular weight	466:485	the molecular weight of alginate	466:497	Therefore, the objective of the current study is to compare the effects of γ-irradiation (dry state, 20-80 kGy), partial (1 and 4%) periodate oxidation (aqueous solution), and autoclaving (dry state) on the molecular weight of alginate, as well as the degradation behavior of alginate-based composites.
24436203	0	28	dep	weight	61:66	arg1	the					47:49	the	47:49	the	47:49	Effects of physical and chemical treatments on the molecular weight and degradation of alginate-hydroxyapatite composites.
24436203	2	29	theme	periodate	395:403	arg1	oxidation					405:413	partial (1 and 4%) periodate oxidation	376:413	partial (1 and 4%) periodate oxidation (aqueous solution)	376:432	Therefore, the objective of the current study is to compare the effects of γ-irradiation (dry state, 20-80 kGy), partial (1 and 4%) periodate oxidation (aqueous solution), and autoclaving (dry state) on the molecular weight of alginate, as well as the degradation behavior of alginate-based composites.
24436203	2	29	theme	periodate	395:403	arg1	solution					424:431	aqueous solution	416:431	aqueous solution	416:431	Therefore, the objective of the current study is to compare the effects of γ-irradiation (dry state, 20-80 kGy), partial (1 and 4%) periodate oxidation (aqueous solution), and autoclaving (dry state) on the molecular weight of alginate, as well as the degradation behavior of alginate-based composites.
24436203	2	30	theme	oxidation	405:413	arg1	behavior					527:534	the degradation behavior	511:534	the degradation behavior of alginate-based composites	511:563	Therefore, the objective of the current study is to compare the effects of γ-irradiation (dry state, 20-80 kGy), partial (1 and 4%) periodate oxidation (aqueous solution), and autoclaving (dry state) on the molecular weight of alginate, as well as the degradation behavior of alginate-based composites.
24436203	2	30	theme	oxidation	405:413	arg1	effects					327:333	the effects	323:333	the effects of γ-irradiation (dry state, 20-80 kGy), partial (1 and 4%) periodate oxidation (aqueous solution), and autoclaving (dry state) on the molecular weight of alginate	323:497	Therefore, the objective of the current study is to compare the effects of γ-irradiation (dry state, 20-80 kGy), partial (1 and 4%) periodate oxidation (aqueous solution), and autoclaving (dry state) on the molecular weight of alginate, as well as the degradation behavior of alginate-based composites.
24436203	3	31	theme	rapid	699:703	arg1	loss					705:708	rapid loss	699:708	rapid loss of composite integrity upon soaking in simulated body fluid	699:768	The results show that γ-irradiation is by far the most destructive technique characterized by strongly reduced molecular weights and rapid loss of composite integrity upon soaking in simulated body fluid.
24436203	2	32	theme	autoclaving	439:449	arg1	behavior					527:534	the degradation behavior	511:534	the degradation behavior of alginate-based composites	511:563	Therefore, the objective of the current study is to compare the effects of γ-irradiation (dry state, 20-80 kGy), partial (1 and 4%) periodate oxidation (aqueous solution), and autoclaving (dry state) on the molecular weight of alginate, as well as the degradation behavior of alginate-based composites.
24436203	2	32	theme	autoclaving	439:449	arg1	effects					327:333	the effects	323:333	the effects of γ-irradiation (dry state, 20-80 kGy), partial (1 and 4%) periodate oxidation (aqueous solution), and autoclaving (dry state) on the molecular weight of alginate	323:497	Therefore, the objective of the current study is to compare the effects of γ-irradiation (dry state, 20-80 kGy), partial (1 and 4%) periodate oxidation (aqueous solution), and autoclaving (dry state) on the molecular weight of alginate, as well as the degradation behavior of alginate-based composites.
24436203	5	33	theme	alginate-hydroxyapatite	1161:1183	arg1	composites					1185:1194	the resulting alginate-hydroxyapatite composites	1147:1194	the resulting alginate-hydroxyapatite composites	1147:1194	Autoclaving is shown to be a powerful tool to reduce the molecular weight of alginate in a controllable and mild manner without compromising the integrity of the resulting alginate-hydroxyapatite composites, simply by increasing the number of repetitive autoclaving cycles.
24436203	2	34	theme	alginate	490:497	arg1	weight					480:485	the molecular weight	466:485	the molecular weight of alginate	466:497	Therefore, the objective of the current study is to compare the effects of γ-irradiation (dry state, 20-80 kGy), partial (1 and 4%) periodate oxidation (aqueous solution), and autoclaving (dry state) on the molecular weight of alginate, as well as the degradation behavior of alginate-based composites.
24436203	4	35	theme	periodate	779:787	arg1	oxidation					789:797	Partial periodate oxidation	771:797	Partial periodate oxidation	771:797	Partial periodate oxidation is less destructive as characterized by more moderate decreases in molecular weight, but the production of hydrolytically labile bonds compromises the integrity of the resulting composites.
24436203	0	36	theme	chemical	24:31	arg1	treatments					33:42	physical and chemical treatments	11:42	physical and chemical treatments	11:42	Effects of physical and chemical treatments on the molecular weight and degradation of alginate-hydroxyapatite composites.
24436203	4	37	theme	resulting	967:975	arg1	composites					977:986	the resulting composites	963:986	the resulting composites	963:986	Partial periodate oxidation is less destructive as characterized by more moderate decreases in molecular weight, but the production of hydrolytically labile bonds compromises the integrity of the resulting composites.
24436203	5	38	theme	molecular	1046:1054	arg1	weight					1056:1061	the molecular weight	1042:1061	the molecular weight of alginate	1042:1073	Autoclaving is shown to be a powerful tool to reduce the molecular weight of alginate in a controllable and mild manner without compromising the integrity of the resulting alginate-hydroxyapatite composites, simply by increasing the number of repetitive autoclaving cycles.
24436203	4	39	theme	Partial	771:777	arg1	oxidation					789:797	Partial periodate oxidation	771:797	Partial periodate oxidation	771:797	Partial periodate oxidation is less destructive as characterized by more moderate decreases in molecular weight, but the production of hydrolytically labile bonds compromises the integrity of the resulting composites.
24436203	1	40	theme	alginate-based	237:250	arg1	materials					252:260	alginate-based materials	237:260	alginate-based materials	237:260	Degradation of alginate remains a critical issue to allow predictable biological performance upon implantation of alginate-based materials.
24436203	4	41	theme	labile	921:926	arg1	bonds					928:932	hydrolytically labile bonds	906:932	hydrolytically labile bonds	906:932	Partial periodate oxidation is less destructive as characterized by more moderate decreases in molecular weight, but the production of hydrolytically labile bonds compromises the integrity of the resulting composites.
24436203	2	42	theme	degradation	515:525	arg1	behavior					527:534	the degradation behavior	511:534	the degradation behavior of alginate-based composites	511:563	Therefore, the objective of the current study is to compare the effects of γ-irradiation (dry state, 20-80 kGy), partial (1 and 4%) periodate oxidation (aqueous solution), and autoclaving (dry state) on the molecular weight of alginate, as well as the degradation behavior of alginate-based composites.
24436203	3	43	theme	composite	713:721	arg1	integrity					723:731	composite integrity	713:731	composite integrity	713:731	The results show that γ-irradiation is by far the most destructive technique characterized by strongly reduced molecular weights and rapid loss of composite integrity upon soaking in simulated body fluid.
24436203	3	44	theme	integrity	723:731	arg1	weights					687:693	strongly reduced molecular weights	660:693	strongly reduced molecular weights	660:693	The results show that γ-irradiation is by far the most destructive technique characterized by strongly reduced molecular weights and rapid loss of composite integrity upon soaking in simulated body fluid.
24436203	3	44	theme	integrity	723:731	arg1	loss					705:708	rapid loss	699:708	rapid loss of composite integrity upon soaking in simulated body fluid	699:768	The results show that γ-irradiation is by far the most destructive technique characterized by strongly reduced molecular weights and rapid loss of composite integrity upon soaking in simulated body fluid.
24436203	5	45	theme	controllable	1080:1091	arg1	manner					1102:1107	a controllable and mild manner	1078:1107	a controllable and mild manner	1078:1107	Autoclaving is shown to be a powerful tool to reduce the molecular weight of alginate in a controllable and mild manner without compromising the integrity of the resulting alginate-hydroxyapatite composites, simply by increasing the number of repetitive autoclaving cycles.
24436203	5	46	theme	powerful	1018:1025	arg1	tool					1027:1030	a powerful tool	1016:1030	a powerful tool to reduce the molecular weight of alginate in a controllable and mild manner without compromising the integrity of the resulting alginate-hydroxyapatite composites, simply by increasing the number of repetitive autoclaving cycles	1016:1260	Autoclaving is shown to be a powerful tool to reduce the molecular weight of alginate in a controllable and mild manner without compromising the integrity of the resulting alginate-hydroxyapatite composites, simply by increasing the number of repetitive autoclaving cycles.
24436203	5	46	theme	powerful	1018:1025	arg1	Autoclaving					989:999	Autoclaving	989:999	Autoclaving	989:999	Autoclaving is shown to be a powerful tool to reduce the molecular weight of alginate in a controllable and mild manner without compromising the integrity of the resulting alginate-hydroxyapatite composites, simply by increasing the number of repetitive autoclaving cycles.
24436203	2	47	theme	dry	353:355	arg1	γ-irradiation					338:350	γ-irradiation	338:350	γ-irradiation (dry state, 20-80 kGy)	338:373	Therefore, the objective of the current study is to compare the effects of γ-irradiation (dry state, 20-80 kGy), partial (1 and 4%) periodate oxidation (aqueous solution), and autoclaving (dry state) on the molecular weight of alginate, as well as the degradation behavior of alginate-based composites.
24436203	2	47	theme	dry	353:355	arg1	state					357:361	dry state	353:361	dry state	353:361	Therefore, the objective of the current study is to compare the effects of γ-irradiation (dry state, 20-80 kGy), partial (1 and 4%) periodate oxidation (aqueous solution), and autoclaving (dry state) on the molecular weight of alginate, as well as the degradation behavior of alginate-based composites.
24436203	3	48	theme	destructive	621:631	arg1	technique					633:641	the most destructive technique	612:641	the most destructive technique characterized by strongly reduced molecular weights and rapid loss of composite integrity upon soaking in simulated body fluid	612:768	The results show that γ-irradiation is by far the most destructive technique characterized by strongly reduced molecular weights and rapid loss of composite integrity upon soaking in simulated body fluid.
24436203	3	48	theme	destructive	621:631	arg1	γ-irradiation					588:600	γ-irradiation	588:600	γ-irradiation	588:600	The results show that γ-irradiation is by far the most destructive technique characterized by strongly reduced molecular weights and rapid loss of composite integrity upon soaking in simulated body fluid.
24436203	1	49	theme	alginate	138:145	arg1	Degradation					123:133	Degradation	123:133	Degradation of alginate	123:145	Degradation of alginate remains a critical issue to allow predictable biological performance upon implantation of alginate-based materials.
24436203	2	50	dep	state	357:361	arg1	20-80 kGy					364:372	20-80 kGy	364:372	20-80 kGy	364:372	Therefore, the objective of the current study is to compare the effects of γ-irradiation (dry state, 20-80 kGy), partial (1 and 4%) periodate oxidation (aqueous solution), and autoclaving (dry state) on the molecular weight of alginate, as well as the degradation behavior of alginate-based composites.
24436203	1	51	theme	materials	252:260	arg1	implantation					221:232	implantation	221:232	implantation of alginate-based materials	221:260	Degradation of alginate remains a critical issue to allow predictable biological performance upon implantation of alginate-based materials.
24436203	4	52	theme	composites	977:986	arg1	integrity					950:958	the integrity	946:958	the integrity of the resulting composites	946:986	Partial periodate oxidation is less destructive as characterized by more moderate decreases in molecular weight, but the production of hydrolytically labile bonds compromises the integrity of the resulting composites.
24436203	2	53	dep	partial	376:382	arg1	%					392:392	1 and 4%	385:392	1 and 4%	385:392	Therefore, the objective of the current study is to compare the effects of γ-irradiation (dry state, 20-80 kGy), partial (1 and 4%) periodate oxidation (aqueous solution), and autoclaving (dry state) on the molecular weight of alginate, as well as the degradation behavior of alginate-based composites.
24436203	2	54	theme	γ-irradiation	338:350	arg1	behavior					527:534	the degradation behavior	511:534	the degradation behavior of alginate-based composites	511:563	Therefore, the objective of the current study is to compare the effects of γ-irradiation (dry state, 20-80 kGy), partial (1 and 4%) periodate oxidation (aqueous solution), and autoclaving (dry state) on the molecular weight of alginate, as well as the degradation behavior of alginate-based composites.
24436203	2	54	theme	γ-irradiation	338:350	arg1	effects					327:333	the effects	323:333	the effects of γ-irradiation (dry state, 20-80 kGy), partial (1 and 4%) periodate oxidation (aqueous solution), and autoclaving (dry state) on the molecular weight of alginate	323:497	Therefore, the objective of the current study is to compare the effects of γ-irradiation (dry state, 20-80 kGy), partial (1 and 4%) periodate oxidation (aqueous solution), and autoclaving (dry state) on the molecular weight of alginate, as well as the degradation behavior of alginate-based composites.
24436203	3	55	theme	simulated	749:757	arg1	fluid					764:768	simulated body fluid	749:768	simulated body fluid	749:768	The results show that γ-irradiation is by far the most destructive technique characterized by strongly reduced molecular weights and rapid loss of composite integrity upon soaking in simulated body fluid.
24436203	0	56	theme	molecular	51:59	arg1	weight					61:66	molecular weight	51:66	molecular weight	51:66	Effects of physical and chemical treatments on the molecular weight and degradation of alginate-hydroxyapatite composites.
24436203	4	57	from	decreases	853:861	arg1	weight					876:881	molecular weight	866:881	molecular weight	866:881	Partial periodate oxidation is less destructive as characterized by more moderate decreases in molecular weight, but the production of hydrolytically labile bonds compromises the integrity of the resulting composites.
24436203	0	58	theme	physical	11:18	arg1	treatments					33:42	physical and chemical treatments	11:42	physical and chemical treatments	11:42	Effects of physical and chemical treatments on the molecular weight and degradation of alginate-hydroxyapatite composites.
24436203	1	59	theme	critical	157:164	arg1	issue					166:170	a critical issue	155:170	a critical issue to allow predictable biological performance upon implantation of alginate-based materials	155:260	Degradation of alginate remains a critical issue to allow predictable biological performance upon implantation of alginate-based materials.
24436203	5	60	theme	repetitive	1232:1241	arg1	cycles					1255:1260	repetitive autoclaving cycles	1232:1260	repetitive autoclaving cycles	1232:1260	Autoclaving is shown to be a powerful tool to reduce the molecular weight of alginate in a controllable and mild manner without compromising the integrity of the resulting alginate-hydroxyapatite composites, simply by increasing the number of repetitive autoclaving cycles.
24436203	3	61	theme	body	759:762	arg1	fluid					764:768	simulated body fluid	749:768	simulated body fluid	749:768	The results show that γ-irradiation is by far the most destructive technique characterized by strongly reduced molecular weights and rapid loss of composite integrity upon soaking in simulated body fluid.
26836293	10	0	theme	material	2168:2175	arg1	content					2143:2149	The mineral content	2131:2149	The mineral content of the composite material	2131:2175	The mineral content of the composite material converted from brushite into hydroxyapatite when submerged in simulated body fluid, indicating possible bioactivity.
26836293	3	1	theme	necessary	711:719	arg1	degree					721:726	the necessary degree	707:726	the necessary degree of control	707:737	However, published preparation methods of brushite composites, in which the mineral phase is precipitated within the hydrogel network, fail to offer the necessary degree of control over the mineral phase, content and distribution within the hydrogel matrix.
26836293	4	2	theme	precise	897:903	arg1	parameters					917:926	the precise fabrication parameters	893:926	the precise fabrication parameters needed to prepare composites with controlled composition and properties	893:998	The main focus of this study is to address these shortcomings by determining the precise fabrication parameters needed to prepare composites with controlled composition and properties.
26836293	11	3	theme	tissue	2440:2445	arg1	engineering					2447:2457	bone tissue engineering	2435:2457	bone tissue engineering	2435:2457	Additionally, initial cell culture studies revealed that both the material and the synthesis procedure are compatible with cells relevant to bone tissue engineering.
26836293	3	4	theme	mineral	748:754	arg1	distribution					775:786	distribution	775:786	distribution	775:786	However, published preparation methods of brushite composites, in which the mineral phase is precipitated within the hydrogel network, fail to offer the necessary degree of control over the mineral phase, content and distribution within the hydrogel matrix.
26836293	3	4	theme	mineral	748:754	arg1	content					763:769	content	763:769	content	763:769	However, published preparation methods of brushite composites, in which the mineral phase is precipitated within the hydrogel network, fail to offer the necessary degree of control over the mineral phase, content and distribution within the hydrogel matrix.
26836293	3	4	theme	mineral	748:754	arg1	phase					756:760	the mineral phase	744:760	the mineral phase	744:760	However, published preparation methods of brushite composites, in which the mineral phase is precipitated within the hydrogel network, fail to offer the necessary degree of control over the mineral phase, content and distribution within the hydrogel matrix.
26836293	1	5	theme	bone	291:294	arg1	applications					309:320	bone regeneration applications	291:320	bone regeneration applications	291:320	Due to high solubility and fast resorption behaviour under physiological conditions, brushite (CaHPO4⋅2H2O, calcium monohydrogen phosphate dihydrate, dicalcium phosphate dihydrate) has great potential in bone regeneration applications, both in combination with scaffolds or as a component of calcium phosphate cements.
26836293	1	6	with	combination	331:341	arg1	scaffolds					348:356	scaffolds	348:356	scaffolds	348:356	Due to high solubility and fast resorption behaviour under physiological conditions, brushite (CaHPO4⋅2H2O, calcium monohydrogen phosphate dihydrate, dicalcium phosphate dihydrate) has great potential in bone regeneration applications, both in combination with scaffolds or as a component of calcium phosphate cements.
26836293	6	7	theme	phase	1235:1239	arg1	nucleation					1203:1212	Reliable nucleation	1194:1212	Reliable nucleation of a desired mineral phase within the alginate network	1194:1267	Reliable nucleation of a desired mineral phase within the alginate network proved more challenging than simple aqueous precipitation.
26836293	3	8	theme	published	567:575	arg1	methods					589:595	published preparation methods	567:595	published preparation methods	567:595	However, published preparation methods of brushite composites, in which the mineral phase is precipitated within the hydrogel network, fail to offer the necessary degree of control over the mineral phase, content and distribution within the hydrogel matrix.
26836293	8	9	theme	mineral	1789:1795	arg1	mineral					1789:1795	mineral	1789:1795	mineral	1789:1795	To overcome this, the incorporation of brushite seed crystals resulted in good control during the mineral phase, and by adjusting the number of seeds and amount of precursor concentration, the amount of mineral could be tuned.
26836293	8	9	theme	mineral	1789:1795	arg1	amount					1779:1784	the amount	1775:1784	the amount of mineral	1775:1795	To overcome this, the incorporation of brushite seed crystals resulted in good control during the mineral phase, and by adjusting the number of seeds and amount of precursor concentration, the amount of mineral could be tuned.
26836293	2	10	with	combination	429:439	arg1	hydrogels					446:454	hydrogels	446:454	hydrogels	446:454	The use of brushite in combination with hydrogels opens up possibilities for new cell-based tissue engineering applications of this promising material.
26836293	7	11	theme	phases	1497:1502	arg1	nucleation					1477:1486	the nucleation	1473:1486	the nucleation of other phases such as hydroxyapatite and octacalcium phosphate, which would otherwise not form	1473:1583	This was largely due to ion transport within the hydrogel producing concentration gradients that modified levels of supersaturation and favoured the nucleation of other phases such as hydroxyapatite and octacalcium phosphate, which would otherwise not form.
26836293	1	12	theme	monohydrogen	203:214	arg1	dihydrate					226:234	calcium monohydrogen phosphate dihydrate	195:234	calcium monohydrogen phosphate dihydrate	195:234	Due to high solubility and fast resorption behaviour under physiological conditions, brushite (CaHPO4⋅2H2O, calcium monohydrogen phosphate dihydrate, dicalcium phosphate dihydrate) has great potential in bone regeneration applications, both in combination with scaffolds or as a component of calcium phosphate cements.
26836293	1	12	theme	monohydrogen	203:214	arg1	CaHPO4⋅2H2O					182:192	CaHPO4⋅2H2O	182:192	CaHPO4⋅2H2O	182:192	Due to high solubility and fast resorption behaviour under physiological conditions, brushite (CaHPO4⋅2H2O, calcium monohydrogen phosphate dihydrate, dicalcium phosphate dihydrate) has great potential in bone regeneration applications, both in combination with scaffolds or as a component of calcium phosphate cements.
26836293	9	13	theme	thermogravimetric	2016:2032	arg1	analysis					2034:2041	thermogravimetric analysis	2016:2041	thermogravimetric analysis	2016:2041	The material was characterised with a range of physical techniques, including scanning electron microscopy, powder x-ray diffraction and Rietveld refinement, Fourier transform infrared spectroscopy, and thermogravimetric analysis, in order to assess the mineral morphology, phase and amount within the organic matrix.
26836293	3	14	theme	brushite	600:607	arg1	composites					609:618	brushite composites	600:618	brushite composites	600:618	However, published preparation methods of brushite composites, in which the mineral phase is precipitated within the hydrogel network, fail to offer the necessary degree of control over the mineral phase, content and distribution within the hydrogel matrix.
26836293	4	15	theme	controlled	962:971	arg1	composition					973:983	controlled composition	962:983	controlled composition	962:983	The main focus of this study is to address these shortcomings by determining the precise fabrication parameters needed to prepare composites with controlled composition and properties.
26836293	11	16	theme	bone	2435:2438	arg1	engineering					2447:2457	bone tissue engineering	2435:2457	bone tissue engineering	2435:2457	Additionally, initial cell culture studies revealed that both the material and the synthesis procedure are compatible with cells relevant to bone tissue engineering.
26836293	6	17	theme	aqueous	1305:1311	arg1	precipitation					1313:1325	simple aqueous precipitation	1298:1325	simple aqueous precipitation	1298:1325	Reliable nucleation of a desired mineral phase within the alginate network proved more challenging than simple aqueous precipitation.
26836293	3	18	theme	hydrogel	799:806	arg1	matrix					808:813	the hydrogel matrix	795:813	the hydrogel matrix	795:813	However, published preparation methods of brushite composites, in which the mineral phase is precipitated within the hydrogel network, fail to offer the necessary degree of control over the mineral phase, content and distribution within the hydrogel matrix.
26836293	5	19	theme	hydrogel	1136:1143	arg1	crosslinking					1116:1127	the simultaneous crosslinking	1099:1127	the simultaneous crosslinking of the hydrogel and precipitation of an inorganic mineral phase	1099:1191	Composite alginate microbeads were prepared using a counter-diffusion technique, which allows for the simultaneous crosslinking of the hydrogel and precipitation of an inorganic mineral phase.
26836293	3	20	theme	mineral	634:640	arg1	precipitated					651:662	precipitated	651:662	precipitated	651:662	However, published preparation methods of brushite composites, in which the mineral phase is precipitated within the hydrogel network, fail to offer the necessary degree of control over the mineral phase, content and distribution within the hydrogel matrix.
26836293	3	20	theme	mineral	634:640	arg1	phase					642:646	the mineral phase	630:646	the mineral phase	630:646	However, published preparation methods of brushite composites, in which the mineral phase is precipitated within the hydrogel network, fail to offer the necessary degree of control over the mineral phase, content and distribution within the hydrogel matrix.
26836293	1	21	theme	dicalcium	237:245	arg1	CaHPO4⋅2H2O					182:192	CaHPO4⋅2H2O	182:192	CaHPO4⋅2H2O	182:192	Due to high solubility and fast resorption behaviour under physiological conditions, brushite (CaHPO4⋅2H2O, calcium monohydrogen phosphate dihydrate, dicalcium phosphate dihydrate) has great potential in bone regeneration applications, both in combination with scaffolds or as a component of calcium phosphate cements.
26836293	1	21	theme	dicalcium	237:245	arg1	dihydrate					257:265	dicalcium phosphate dihydrate	237:265	dicalcium phosphate dihydrate	237:265	Due to high solubility and fast resorption behaviour under physiological conditions, brushite (CaHPO4⋅2H2O, calcium monohydrogen phosphate dihydrate, dicalcium phosphate dihydrate) has great potential in bone regeneration applications, both in combination with scaffolds or as a component of calcium phosphate cements.
26836293	9	22	dep	transform	1979:1987	arg1	infrared					1989:1996	infrared	1989:1996	transform infrared spectroscopy	1979:2009	The material was characterised with a range of physical techniques, including scanning electron microscopy, powder x-ray diffraction and Rietveld refinement, Fourier transform infrared spectroscopy, and thermogravimetric analysis, in order to assess the mineral morphology, phase and amount within the organic matrix.
26836293	10	23	theme	body	2249:2252	arg1	fluid					2254:2258	simulated body fluid	2239:2258	simulated body fluid	2239:2258	The mineral content of the composite material converted from brushite into hydroxyapatite when submerged in simulated body fluid, indicating possible bioactivity.
26836293	5	24	theme	simultaneous	1103:1114	arg1	crosslinking					1116:1127	the simultaneous crosslinking	1099:1127	the simultaneous crosslinking of the hydrogel and precipitation of an inorganic mineral phase	1099:1191	Composite alginate microbeads were prepared using a counter-diffusion technique, which allows for the simultaneous crosslinking of the hydrogel and precipitation of an inorganic mineral phase.
26836293	1	25	theme	physiological	146:158	arg1	conditions					160:169	physiological conditions	146:169	physiological conditions	146:169	Due to high solubility and fast resorption behaviour under physiological conditions, brushite (CaHPO4⋅2H2O, calcium monohydrogen phosphate dihydrate, dicalcium phosphate dihydrate) has great potential in bone regeneration applications, both in combination with scaffolds or as a component of calcium phosphate cements.
26836293	11	26	theme	cell	2316:2319	arg1	studies					2329:2335	initial cell culture studies	2308:2335	initial cell culture studies	2308:2335	Additionally, initial cell culture studies revealed that both the material and the synthesis procedure are compatible with cells relevant to bone tissue engineering.
26836293	9	27	theme	mineral	2067:2073	arg1	morphology					2075:2084	the mineral morphology	2063:2084	the mineral morphology	2063:2084	The material was characterised with a range of physical techniques, including scanning electron microscopy, powder x-ray diffraction and Rietveld refinement, Fourier transform infrared spectroscopy, and thermogravimetric analysis, in order to assess the mineral morphology, phase and amount within the organic matrix.
26836293	5	28	theme	mineral	1179:1185	arg1	phase					1187:1191	an inorganic mineral phase	1166:1191	an inorganic mineral phase	1166:1191	Composite alginate microbeads were prepared using a counter-diffusion technique, which allows for the simultaneous crosslinking of the hydrogel and precipitation of an inorganic mineral phase.
26836293	10	29	theme	possible	2272:2279	arg1	bioactivity					2281:2291	possible bioactivity	2272:2291	possible bioactivity	2272:2291	The mineral content of the composite material converted from brushite into hydroxyapatite when submerged in simulated body fluid, indicating possible bioactivity.
26836293	3	30	from	methods	589:595	arg1	precipitated					651:662	precipitated	651:662	precipitated	651:662	However, published preparation methods of brushite composites, in which the mineral phase is precipitated within the hydrogel network, fail to offer the necessary degree of control over the mineral phase, content and distribution within the hydrogel matrix.
26836293	3	30	from	methods	589:595	arg1	phase					642:646	the mineral phase	630:646	the mineral phase	630:646	However, published preparation methods of brushite composites, in which the mineral phase is precipitated within the hydrogel network, fail to offer the necessary degree of control over the mineral phase, content and distribution within the hydrogel matrix.
26836293	5	31	theme	precipitation	1149:1161	arg1	crosslinking					1116:1127	the simultaneous crosslinking	1099:1127	the simultaneous crosslinking of the hydrogel and precipitation of an inorganic mineral phase	1099:1191	Composite alginate microbeads were prepared using a counter-diffusion technique, which allows for the simultaneous crosslinking of the hydrogel and precipitation of an inorganic mineral phase.
26836293	5	32	theme	alginate	1011:1018	arg1	microbeads					1020:1029	Composite alginate microbeads	1001:1029	Composite alginate microbeads	1001:1029	Composite alginate microbeads were prepared using a counter-diffusion technique, which allows for the simultaneous crosslinking of the hydrogel and precipitation of an inorganic mineral phase.
26836293	2	33	theme	material	548:555	arg1	applications					517:528	new cell-based tissue engineering applications	483:528	new cell-based tissue engineering applications of this promising material	483:555	The use of brushite in combination with hydrogels opens up possibilities for new cell-based tissue engineering applications of this promising material.
26836293	1	34	theme	phosphate	387:395	arg1	component					366:374	a component	364:374	a component of calcium phosphate cements	364:403	Due to high solubility and fast resorption behaviour under physiological conditions, brushite (CaHPO4⋅2H2O, calcium monohydrogen phosphate dihydrate, dicalcium phosphate dihydrate) has great potential in bone regeneration applications, both in combination with scaffolds or as a component of calcium phosphate cements.
26836293	9	35	theme	scanning	1891:1898	arg1	microscopy					1909:1918	scanning electron microscopy	1891:1918	scanning electron microscopy	1891:1918	The material was characterised with a range of physical techniques, including scanning electron microscopy, powder x-ray diffraction and Rietveld refinement, Fourier transform infrared spectroscopy, and thermogravimetric analysis, in order to assess the mineral morphology, phase and amount within the organic matrix.
26836293	4	36	with	composites	946:955	arg1	properties					989:998	properties	989:998	properties	989:998	The main focus of this study is to address these shortcomings by determining the precise fabrication parameters needed to prepare composites with controlled composition and properties.
26836293	4	36	with	composites	946:955	arg1	composition					973:983	controlled composition	962:983	controlled composition	962:983	The main focus of this study is to address these shortcomings by determining the precise fabrication parameters needed to prepare composites with controlled composition and properties.
26836293	9	37	theme	physical	1860:1867	arg1	analysis					2034:2041	thermogravimetric analysis	2016:2041	thermogravimetric analysis	2016:2041	The material was characterised with a range of physical techniques, including scanning electron microscopy, powder x-ray diffraction and Rietveld refinement, Fourier transform infrared spectroscopy, and thermogravimetric analysis, in order to assess the mineral morphology, phase and amount within the organic matrix.
26836293	9	37	theme	physical	1860:1867	arg1	diffraction					1934:1944	powder x-ray diffraction	1921:1944	powder x-ray diffraction	1921:1944	The material was characterised with a range of physical techniques, including scanning electron microscopy, powder x-ray diffraction and Rietveld refinement, Fourier transform infrared spectroscopy, and thermogravimetric analysis, in order to assess the mineral morphology, phase and amount within the organic matrix.
26836293	9	37	theme	physical	1860:1867	arg1	microscopy					1909:1918	scanning electron microscopy	1891:1918	scanning electron microscopy	1891:1918	The material was characterised with a range of physical techniques, including scanning electron microscopy, powder x-ray diffraction and Rietveld refinement, Fourier transform infrared spectroscopy, and thermogravimetric analysis, in order to assess the mineral morphology, phase and amount within the organic matrix.
26836293	9	37	theme	physical	1860:1867	arg1	refinement					1959:1968	Rietveld refinement	1950:1968	Rietveld refinement	1950:1968	The material was characterised with a range of physical techniques, including scanning electron microscopy, powder x-ray diffraction and Rietveld refinement, Fourier transform infrared spectroscopy, and thermogravimetric analysis, in order to assess the mineral morphology, phase and amount within the organic matrix.
26836293	9	37	theme	physical	1860:1867	arg1	techniques					1869:1878	physical techniques	1860:1878	physical techniques	1860:1878	The material was characterised with a range of physical techniques, including scanning electron microscopy, powder x-ray diffraction and Rietveld refinement, Fourier transform infrared spectroscopy, and thermogravimetric analysis, in order to assess the mineral morphology, phase and amount within the organic matrix.
26836293	9	37	theme	physical	1860:1867	arg1	Fourier					1971:1977	Fourier	1971:1977	Fourier transform infrared spectroscopy	1971:2009	The material was characterised with a range of physical techniques, including scanning electron microscopy, powder x-ray diffraction and Rietveld refinement, Fourier transform infrared spectroscopy, and thermogravimetric analysis, in order to assess the mineral morphology, phase and amount within the organic matrix.
26836293	9	38	theme	organic	2115:2121	arg1	matrix					2123:2128	the organic matrix	2111:2128	the organic matrix	2111:2128	The material was characterised with a range of physical techniques, including scanning electron microscopy, powder x-ray diffraction and Rietveld refinement, Fourier transform infrared spectroscopy, and thermogravimetric analysis, in order to assess the mineral morphology, phase and amount within the organic matrix.
26836293	1	39	dep	brushite	172:179	arg1	dihydrate					226:234	calcium monohydrogen phosphate dihydrate	195:234	calcium monohydrogen phosphate dihydrate	195:234	Due to high solubility and fast resorption behaviour under physiological conditions, brushite (CaHPO4⋅2H2O, calcium monohydrogen phosphate dihydrate, dicalcium phosphate dihydrate) has great potential in bone regeneration applications, both in combination with scaffolds or as a component of calcium phosphate cements.
26836293	1	39	dep	brushite	172:179	arg1	CaHPO4⋅2H2O					182:192	CaHPO4⋅2H2O	182:192	CaHPO4⋅2H2O	182:192	Due to high solubility and fast resorption behaviour under physiological conditions, brushite (CaHPO4⋅2H2O, calcium monohydrogen phosphate dihydrate, dicalcium phosphate dihydrate) has great potential in bone regeneration applications, both in combination with scaffolds or as a component of calcium phosphate cements.
26836293	1	39	dep	brushite	172:179	arg1	dihydrate					257:265	dicalcium phosphate dihydrate	237:265	dicalcium phosphate dihydrate	237:265	Due to high solubility and fast resorption behaviour under physiological conditions, brushite (CaHPO4⋅2H2O, calcium monohydrogen phosphate dihydrate, dicalcium phosphate dihydrate) has great potential in bone regeneration applications, both in combination with scaffolds or as a component of calcium phosphate cements.
26836293	1	40	theme	regeneration	296:307	arg1	applications					309:320	bone regeneration applications	291:320	bone regeneration applications	291:320	Due to high solubility and fast resorption behaviour under physiological conditions, brushite (CaHPO4⋅2H2O, calcium monohydrogen phosphate dihydrate, dicalcium phosphate dihydrate) has great potential in bone regeneration applications, both in combination with scaffolds or as a component of calcium phosphate cements.
26836293	7	41	theme	concentration	1396:1408	arg1	gradients					1410:1418	concentration gradients	1396:1418	concentration gradients that modified levels of supersaturation and favoured the nucleation of other phases such as hydroxyapatite and octacalcium phosphate, which would otherwise not form	1396:1583	This was largely due to ion transport within the hydrogel producing concentration gradients that modified levels of supersaturation and favoured the nucleation of other phases such as hydroxyapatite and octacalcium phosphate, which would otherwise not form.
26836293	8	42	theme	seed	1634:1637	arg1	crystals					1639:1646	brushite seed crystals	1625:1646	brushite seed crystals	1625:1646	To overcome this, the incorporation of brushite seed crystals resulted in good control during the mineral phase, and by adjusting the number of seeds and amount of precursor concentration, the amount of mineral could be tuned.
26836293	1	43	theme	fast	114:117	arg1	behaviour					130:138	fast resorption behaviour	114:138	fast resorption behaviour	114:138	Due to high solubility and fast resorption behaviour under physiological conditions, brushite (CaHPO4⋅2H2O, calcium monohydrogen phosphate dihydrate, dicalcium phosphate dihydrate) has great potential in bone regeneration applications, both in combination with scaffolds or as a component of calcium phosphate cements.
26836293	2	44	theme	tissue	498:503	arg1	applications					517:528	new cell-based tissue engineering applications	483:528	new cell-based tissue engineering applications of this promising material	483:555	The use of brushite in combination with hydrogels opens up possibilities for new cell-based tissue engineering applications of this promising material.
26836293	9	45	theme	electron	1900:1907	arg1	microscopy					1909:1918	scanning electron microscopy	1891:1918	scanning electron microscopy	1891:1918	The material was characterised with a range of physical techniques, including scanning electron microscopy, powder x-ray diffraction and Rietveld refinement, Fourier transform infrared spectroscopy, and thermogravimetric analysis, in order to assess the mineral morphology, phase and amount within the organic matrix.
26836293	2	46	theme	new	483:485	arg1	applications					517:528	new cell-based tissue engineering applications	483:528	new cell-based tissue engineering applications of this promising material	483:555	The use of brushite in combination with hydrogels opens up possibilities for new cell-based tissue engineering applications of this promising material.
26836293	8	47	theme	good	1660:1663	arg1	control					1665:1671	good control	1660:1671	good control during the mineral phase	1660:1696	To overcome this, the incorporation of brushite seed crystals resulted in good control during the mineral phase, and by adjusting the number of seeds and amount of precursor concentration, the amount of mineral could be tuned.
26836293	3	48	theme	hydrogel	675:682	arg1	network					684:690	the hydrogel network	671:690	the hydrogel network	671:690	However, published preparation methods of brushite composites, in which the mineral phase is precipitated within the hydrogel network, fail to offer the necessary degree of control over the mineral phase, content and distribution within the hydrogel matrix.
26836293	7	49	theme	ion	1352:1354	arg1	transport					1356:1364	ion transport	1352:1364	ion transport within the hydrogel producing concentration gradients that modified levels of supersaturation and favoured the nucleation of other phases such as hydroxyapatite and octacalcium phosphate, which would otherwise not form	1352:1583	This was largely due to ion transport within the hydrogel producing concentration gradients that modified levels of supersaturation and favoured the nucleation of other phases such as hydroxyapatite and octacalcium phosphate, which would otherwise not form.
26836293	9	50	theme	powder	1921:1926	arg1	diffraction					1934:1944	powder x-ray diffraction	1921:1944	powder x-ray diffraction	1921:1944	The material was characterised with a range of physical techniques, including scanning electron microscopy, powder x-ray diffraction and Rietveld refinement, Fourier transform infrared spectroscopy, and thermogravimetric analysis, in order to assess the mineral morphology, phase and amount within the organic matrix.
26836293	1	51	theme	phosphate	247:255	arg1	CaHPO4⋅2H2O					182:192	CaHPO4⋅2H2O	182:192	CaHPO4⋅2H2O	182:192	Due to high solubility and fast resorption behaviour under physiological conditions, brushite (CaHPO4⋅2H2O, calcium monohydrogen phosphate dihydrate, dicalcium phosphate dihydrate) has great potential in bone regeneration applications, both in combination with scaffolds or as a component of calcium phosphate cements.
26836293	1	51	theme	phosphate	247:255	arg1	dihydrate					257:265	dicalcium phosphate dihydrate	237:265	dicalcium phosphate dihydrate	237:265	Due to high solubility and fast resorption behaviour under physiological conditions, brushite (CaHPO4⋅2H2O, calcium monohydrogen phosphate dihydrate, dicalcium phosphate dihydrate) has great potential in bone regeneration applications, both in combination with scaffolds or as a component of calcium phosphate cements.
26836293	10	52	theme	mineral	2135:2141	arg1	content					2143:2149	The mineral content	2131:2149	The mineral content of the composite material	2131:2175	The mineral content of the composite material converted from brushite into hydroxyapatite when submerged in simulated body fluid, indicating possible bioactivity.
26836293	8	53	theme	mineral	1684:1690	arg1	phase					1692:1696	the mineral phase	1680:1696	the mineral phase	1680:1696	To overcome this, the incorporation of brushite seed crystals resulted in good control during the mineral phase, and by adjusting the number of seeds and amount of precursor concentration, the amount of mineral could be tuned.
26836293	5	54	theme	counter-diffusion	1053:1069	arg1	technique					1071:1079	a counter-diffusion technique	1051:1079	a counter-diffusion technique	1051:1079	Composite alginate microbeads were prepared using a counter-diffusion technique, which allows for the simultaneous crosslinking of the hydrogel and precipitation of an inorganic mineral phase.
26836293	9	55	theme	Rietveld	1950:1957	arg1	refinement					1959:1968	Rietveld refinement	1950:1968	Rietveld refinement	1950:1968	The material was characterised with a range of physical techniques, including scanning electron microscopy, powder x-ray diffraction and Rietveld refinement, Fourier transform infrared spectroscopy, and thermogravimetric analysis, in order to assess the mineral morphology, phase and amount within the organic matrix.
26836293	3	56	theme	control	731:737	arg1	degree					721:726	the necessary degree	707:726	the necessary degree of control	707:737	However, published preparation methods of brushite composites, in which the mineral phase is precipitated within the hydrogel network, fail to offer the necessary degree of control over the mineral phase, content and distribution within the hydrogel matrix.
26836293	1	57	theme	great	272:276	arg1	potential					278:286	great potential	272:286	great potential	272:286	Due to high solubility and fast resorption behaviour under physiological conditions, brushite (CaHPO4⋅2H2O, calcium monohydrogen phosphate dihydrate, dicalcium phosphate dihydrate) has great potential in bone regeneration applications, both in combination with scaffolds or as a component of calcium phosphate cements.
26836293	10	58	theme	composite	2158:2166	arg1	material					2168:2175	the composite material	2154:2175	the composite material	2154:2175	The mineral content of the composite material converted from brushite into hydroxyapatite when submerged in simulated body fluid, indicating possible bioactivity.
26836293	3	59	theme	preparation	577:587	arg1	methods					589:595	published preparation methods	567:595	published preparation methods	567:595	However, published preparation methods of brushite composites, in which the mineral phase is precipitated within the hydrogel network, fail to offer the necessary degree of control over the mineral phase, content and distribution within the hydrogel matrix.
26836293	8	60	theme	concentration	1760:1772	arg1	amount					1740:1745	amount	1740:1745	amount of precursor concentration	1740:1772	To overcome this, the incorporation of brushite seed crystals resulted in good control during the mineral phase, and by adjusting the number of seeds and amount of precursor concentration, the amount of mineral could be tuned.
26836293	8	60	theme	concentration	1760:1772	arg1	concentration					1760:1772	precursor concentration	1750:1772	precursor concentration	1750:1772	To overcome this, the incorporation of brushite seed crystals resulted in good control during the mineral phase, and by adjusting the number of seeds and amount of precursor concentration, the amount of mineral could be tuned.
26836293	8	60	theme	concentration	1760:1772	arg1	seeds					1730:1734	seeds	1730:1734	seeds	1730:1734	To overcome this, the incorporation of brushite seed crystals resulted in good control during the mineral phase, and by adjusting the number of seeds and amount of precursor concentration, the amount of mineral could be tuned.
26836293	8	60	theme	concentration	1760:1772	arg1	number					1720:1725	the number	1716:1725	the number of seeds	1716:1734	To overcome this, the incorporation of brushite seed crystals resulted in good control during the mineral phase, and by adjusting the number of seeds and amount of precursor concentration, the amount of mineral could be tuned.
26836293	6	61	theme	desired	1219:1225	arg1	phase					1235:1239	a desired mineral phase	1217:1239	a desired mineral phase	1217:1239	Reliable nucleation of a desired mineral phase within the alginate network proved more challenging than simple aqueous precipitation.
26836293	7	62	theme	octacalcium	1531:1541	arg1	phosphate					1543:1551	octacalcium phosphate	1531:1551	octacalcium phosphate	1531:1551	This was largely due to ion transport within the hydrogel producing concentration gradients that modified levels of supersaturation and favoured the nucleation of other phases such as hydroxyapatite and octacalcium phosphate, which would otherwise not form.
26836293	6	63	theme	mineral	1227:1233	arg1	phase					1235:1239	a desired mineral phase	1217:1239	a desired mineral phase	1217:1239	Reliable nucleation of a desired mineral phase within the alginate network proved more challenging than simple aqueous precipitation.
26836293	11	64	theme	initial	2308:2314	arg1	studies					2329:2335	initial cell culture studies	2308:2335	initial cell culture studies	2308:2335	Additionally, initial cell culture studies revealed that both the material and the synthesis procedure are compatible with cells relevant to bone tissue engineering.
26836293	3	65	theme	composites	609:618	arg1	methods					589:595	published preparation methods	567:595	published preparation methods	567:595	However, published preparation methods of brushite composites, in which the mineral phase is precipitated within the hydrogel network, fail to offer the necessary degree of control over the mineral phase, content and distribution within the hydrogel matrix.
26836293	1	66	theme	calcium	195:201	arg1	dihydrate					226:234	calcium monohydrogen phosphate dihydrate	195:234	calcium monohydrogen phosphate dihydrate	195:234	Due to high solubility and fast resorption behaviour under physiological conditions, brushite (CaHPO4⋅2H2O, calcium monohydrogen phosphate dihydrate, dicalcium phosphate dihydrate) has great potential in bone regeneration applications, both in combination with scaffolds or as a component of calcium phosphate cements.
26836293	1	66	theme	calcium	195:201	arg1	CaHPO4⋅2H2O					182:192	CaHPO4⋅2H2O	182:192	CaHPO4⋅2H2O	182:192	Due to high solubility and fast resorption behaviour under physiological conditions, brushite (CaHPO4⋅2H2O, calcium monohydrogen phosphate dihydrate, dicalcium phosphate dihydrate) has great potential in bone regeneration applications, both in combination with scaffolds or as a component of calcium phosphate cements.
26836293	10	67	theme	simulated	2239:2247	arg1	fluid					2254:2258	simulated body fluid	2239:2258	simulated body fluid	2239:2258	The mineral content of the composite material converted from brushite into hydroxyapatite when submerged in simulated body fluid, indicating possible bioactivity.
26836293	1	68	theme	phosphate	216:224	arg1	dihydrate					226:234	calcium monohydrogen phosphate dihydrate	195:234	calcium monohydrogen phosphate dihydrate	195:234	Due to high solubility and fast resorption behaviour under physiological conditions, brushite (CaHPO4⋅2H2O, calcium monohydrogen phosphate dihydrate, dicalcium phosphate dihydrate) has great potential in bone regeneration applications, both in combination with scaffolds or as a component of calcium phosphate cements.
26836293	1	68	theme	phosphate	216:224	arg1	CaHPO4⋅2H2O					182:192	CaHPO4⋅2H2O	182:192	CaHPO4⋅2H2O	182:192	Due to high solubility and fast resorption behaviour under physiological conditions, brushite (CaHPO4⋅2H2O, calcium monohydrogen phosphate dihydrate, dicalcium phosphate dihydrate) has great potential in bone regeneration applications, both in combination with scaffolds or as a component of calcium phosphate cements.
26836293	7	69	theme	other	1491:1495	arg1	phases					1497:1502	other phases	1491:1502	other phases such as hydroxyapatite and octacalcium phosphate, which would otherwise not form	1491:1583	This was largely due to ion transport within the hydrogel producing concentration gradients that modified levels of supersaturation and favoured the nucleation of other phases such as hydroxyapatite and octacalcium phosphate, which would otherwise not form.
26836293	7	69	theme	other	1491:1495	arg1	hydroxyapatite					1512:1525	hydroxyapatite	1512:1525	hydroxyapatite	1512:1525	This was largely due to ion transport within the hydrogel producing concentration gradients that modified levels of supersaturation and favoured the nucleation of other phases such as hydroxyapatite and octacalcium phosphate, which would otherwise not form.
26836293	7	69	theme	other	1491:1495	arg1	phosphate					1543:1551	octacalcium phosphate	1531:1551	octacalcium phosphate	1531:1551	This was largely due to ion transport within the hydrogel producing concentration gradients that modified levels of supersaturation and favoured the nucleation of other phases such as hydroxyapatite and octacalcium phosphate, which would otherwise not form.
26836293	11	70	theme	synthesis	2377:2385	arg1	procedure					2387:2395	the synthesis procedure	2373:2395	the synthesis procedure	2373:2395	Additionally, initial cell culture studies revealed that both the material and the synthesis procedure are compatible with cells relevant to bone tissue engineering.
26836293	11	70	theme	synthesis	2377:2385	arg1	compatible					2401:2410	compatible	2401:2410	compatible	2401:2410	Additionally, initial cell culture studies revealed that both the material and the synthesis procedure are compatible with cells relevant to bone tissue engineering.
26836293	1	71	contain	has	268:270	arg2	potential					278:286	great potential	272:286	great potential	272:286	Due to high solubility and fast resorption behaviour under physiological conditions, brushite (CaHPO4⋅2H2O, calcium monohydrogen phosphate dihydrate, dicalcium phosphate dihydrate) has great potential in bone regeneration applications, both in combination with scaffolds or as a component of calcium phosphate cements.
26836293	1	71	contain	has	268:270	arg1	brushite					172:179	brushite	172:179	brushite (CaHPO4⋅2H2O, calcium monohydrogen phosphate dihydrate, dicalcium phosphate dihydrate)	172:266	Due to high solubility and fast resorption behaviour under physiological conditions, brushite (CaHPO4⋅2H2O, calcium monohydrogen phosphate dihydrate, dicalcium phosphate dihydrate) has great potential in bone regeneration applications, both in combination with scaffolds or as a component of calcium phosphate cements.
26836293	11	72	theme	relevant	2423:2430	arg1	cells					2417:2421	cells	2417:2421	cells relevant to bone tissue engineering	2417:2457	Additionally, initial cell culture studies revealed that both the material and the synthesis procedure are compatible with cells relevant to bone tissue engineering.
26836293	6	73	theme	simple	1298:1303	arg1	precipitation					1313:1325	simple aqueous precipitation	1298:1325	simple aqueous precipitation	1298:1325	Reliable nucleation of a desired mineral phase within the alginate network proved more challenging than simple aqueous precipitation.
26836293	1	74	theme	resorption	119:128	arg1	behaviour					130:138	fast resorption behaviour	114:138	fast resorption behaviour	114:138	Due to high solubility and fast resorption behaviour under physiological conditions, brushite (CaHPO4⋅2H2O, calcium monohydrogen phosphate dihydrate, dicalcium phosphate dihydrate) has great potential in bone regeneration applications, both in combination with scaffolds or as a component of calcium phosphate cements.
26836293	4	75	theme	fabrication	905:915	arg1	parameters					917:926	the precise fabrication parameters	893:926	the precise fabrication parameters needed to prepare composites with controlled composition and properties	893:998	The main focus of this study is to address these shortcomings by determining the precise fabrication parameters needed to prepare composites with controlled composition and properties.
26836293	7	76	theme	supersaturation	1444:1458	arg1	levels					1434:1439	levels	1434:1439	levels of supersaturation	1434:1458	This was largely due to ion transport within the hydrogel producing concentration gradients that modified levels of supersaturation and favoured the nucleation of other phases such as hydroxyapatite and octacalcium phosphate, which would otherwise not form.
26836293	11	77	theme	culture	2321:2327	arg1	studies					2329:2335	initial cell culture studies	2308:2335	initial cell culture studies	2308:2335	Additionally, initial cell culture studies revealed that both the material and the synthesis procedure are compatible with cells relevant to bone tissue engineering.
26836293	6	78	theme	alginate	1252:1259	arg1	network					1261:1267	the alginate network	1248:1267	the alginate network	1248:1267	Reliable nucleation of a desired mineral phase within the alginate network proved more challenging than simple aqueous precipitation.
26836293	5	79	theme	phase	1187:1191	arg1	precipitation					1149:1161	precipitation	1149:1161	precipitation	1149:1161	Composite alginate microbeads were prepared using a counter-diffusion technique, which allows for the simultaneous crosslinking of the hydrogel and precipitation of an inorganic mineral phase.
26836293	5	79	theme	phase	1187:1191	arg1	hydrogel					1136:1143	hydrogel	1136:1143	hydrogel	1136:1143	Composite alginate microbeads were prepared using a counter-diffusion technique, which allows for the simultaneous crosslinking of the hydrogel and precipitation of an inorganic mineral phase.
26836293	2	80	theme	engineering	505:515	arg1	applications					517:528	new cell-based tissue engineering applications	483:528	new cell-based tissue engineering applications of this promising material	483:555	The use of brushite in combination with hydrogels opens up possibilities for new cell-based tissue engineering applications of this promising material.
26836293	5	81	theme	Composite	1001:1009	arg1	microbeads					1020:1029	Composite alginate microbeads	1001:1029	Composite alginate microbeads	1001:1029	Composite alginate microbeads were prepared using a counter-diffusion technique, which allows for the simultaneous crosslinking of the hydrogel and precipitation of an inorganic mineral phase.
26836293	1	82	theme	calcium	379:385	arg1	phosphate					387:395	calcium phosphate	379:395	calcium phosphate cements	379:403	Due to high solubility and fast resorption behaviour under physiological conditions, brushite (CaHPO4⋅2H2O, calcium monohydrogen phosphate dihydrate, dicalcium phosphate dihydrate) has great potential in bone regeneration applications, both in combination with scaffolds or as a component of calcium phosphate cements.
26836293	2	83	theme	promising	538:546	arg1	material					548:555	this promising material	533:555	this promising material	533:555	The use of brushite in combination with hydrogels opens up possibilities for new cell-based tissue engineering applications of this promising material.
26836293	8	84	theme	brushite	1625:1632	arg1	crystals					1639:1646	brushite seed crystals	1625:1646	brushite seed crystals	1625:1646	To overcome this, the incorporation of brushite seed crystals resulted in good control during the mineral phase, and by adjusting the number of seeds and amount of precursor concentration, the amount of mineral could be tuned.
26836293	3	85	from	precipitated	651:662	arg1	methods					589:595	published preparation methods	567:595	published preparation methods	567:595	However, published preparation methods of brushite composites, in which the mineral phase is precipitated within the hydrogel network, fail to offer the necessary degree of control over the mineral phase, content and distribution within the hydrogel matrix.
26836293	5	86	theme	inorganic	1169:1177	arg1	phase					1187:1191	an inorganic mineral phase	1166:1191	an inorganic mineral phase	1166:1191	Composite alginate microbeads were prepared using a counter-diffusion technique, which allows for the simultaneous crosslinking of the hydrogel and precipitation of an inorganic mineral phase.
26836293	9	87	dep	Fourier	1971:1977	arg1	transform					1979:1987	transform	1979:1987	transform infrared spectroscopy	1979:2009	The material was characterised with a range of physical techniques, including scanning electron microscopy, powder x-ray diffraction and Rietveld refinement, Fourier transform infrared spectroscopy, and thermogravimetric analysis, in order to assess the mineral morphology, phase and amount within the organic matrix.
26836293	1	88	theme	high	94:97	arg1	solubility					99:108	high solubility	94:108	high solubility	94:108	Due to high solubility and fast resorption behaviour under physiological conditions, brushite (CaHPO4⋅2H2O, calcium monohydrogen phosphate dihydrate, dicalcium phosphate dihydrate) has great potential in bone regeneration applications, both in combination with scaffolds or as a component of calcium phosphate cements.
26836293	9	89	theme	techniques	1869:1878	arg1	analysis					2034:2041	thermogravimetric analysis	2016:2041	thermogravimetric analysis	2016:2041	The material was characterised with a range of physical techniques, including scanning electron microscopy, powder x-ray diffraction and Rietveld refinement, Fourier transform infrared spectroscopy, and thermogravimetric analysis, in order to assess the mineral morphology, phase and amount within the organic matrix.
26836293	9	89	theme	techniques	1869:1878	arg1	diffraction					1934:1944	powder x-ray diffraction	1921:1944	powder x-ray diffraction	1921:1944	The material was characterised with a range of physical techniques, including scanning electron microscopy, powder x-ray diffraction and Rietveld refinement, Fourier transform infrared spectroscopy, and thermogravimetric analysis, in order to assess the mineral morphology, phase and amount within the organic matrix.
26836293	9	89	theme	techniques	1869:1878	arg1	range					1851:1855	a range	1849:1855	a range of physical techniques, including scanning electron microscopy, powder x-ray diffraction and Rietveld refinement, Fourier transform infrared spectroscopy, and thermogravimetric analysis	1849:2041	The material was characterised with a range of physical techniques, including scanning electron microscopy, powder x-ray diffraction and Rietveld refinement, Fourier transform infrared spectroscopy, and thermogravimetric analysis, in order to assess the mineral morphology, phase and amount within the organic matrix.
26836293	9	89	theme	techniques	1869:1878	arg1	microscopy					1909:1918	scanning electron microscopy	1891:1918	scanning electron microscopy	1891:1918	The material was characterised with a range of physical techniques, including scanning electron microscopy, powder x-ray diffraction and Rietveld refinement, Fourier transform infrared spectroscopy, and thermogravimetric analysis, in order to assess the mineral morphology, phase and amount within the organic matrix.
26836293	9	89	theme	techniques	1869:1878	arg1	refinement					1959:1968	Rietveld refinement	1950:1968	Rietveld refinement	1950:1968	The material was characterised with a range of physical techniques, including scanning electron microscopy, powder x-ray diffraction and Rietveld refinement, Fourier transform infrared spectroscopy, and thermogravimetric analysis, in order to assess the mineral morphology, phase and amount within the organic matrix.
26836293	9	89	theme	techniques	1869:1878	arg1	Fourier					1971:1977	Fourier	1971:1977	Fourier transform infrared spectroscopy	1971:2009	The material was characterised with a range of physical techniques, including scanning electron microscopy, powder x-ray diffraction and Rietveld refinement, Fourier transform infrared spectroscopy, and thermogravimetric analysis, in order to assess the mineral morphology, phase and amount within the organic matrix.
26836293	8	90	theme	crystals	1639:1646	arg1	incorporation					1608:1620	the incorporation	1604:1620	the incorporation of brushite seed crystals	1604:1646	To overcome this, the incorporation of brushite seed crystals resulted in good control during the mineral phase, and by adjusting the number of seeds and amount of precursor concentration, the amount of mineral could be tuned.
26836293	0	91	theme	brushite	51:58	arg1	recrystallisation					30:46	recrystallisation	30:46	recrystallisation	30:46	Controlled mineralisation and recrystallisation of brushite within alginate hydrogels.
26836293	0	91	theme	brushite	51:58	arg1	mineralisation					11:24	mineralisation	11:24	mineralisation	11:24	Controlled mineralisation and recrystallisation of brushite within alginate hydrogels.
26836293	2	92	theme	cell-based	487:496	arg1	applications					517:528	new cell-based tissue engineering applications	483:528	new cell-based tissue engineering applications of this promising material	483:555	The use of brushite in combination with hydrogels opens up possibilities for new cell-based tissue engineering applications of this promising material.
26836293	1	93	dep	phosphate	387:395	arg1	cements					397:403	cements	397:403	cements	397:403	Due to high solubility and fast resorption behaviour under physiological conditions, brushite (CaHPO4⋅2H2O, calcium monohydrogen phosphate dihydrate, dicalcium phosphate dihydrate) has great potential in bone regeneration applications, both in combination with scaffolds or as a component of calcium phosphate cements.
26836293	2	94	theme	brushite	417:424	arg1	use					410:412	The use	406:412	The use of brushite in combination with hydrogels	406:454	The use of brushite in combination with hydrogels opens up possibilities for new cell-based tissue engineering applications of this promising material.
26836293	0	95	theme	alginate	67:74	arg1	hydrogels					76:84	alginate hydrogels	67:84	alginate hydrogels	67:84	Controlled mineralisation and recrystallisation of brushite within alginate hydrogels.
26836293	11	96	with	compatible	2401:2410	arg1	cells					2417:2421	cells	2417:2421	cells relevant to bone tissue engineering	2417:2457	Additionally, initial cell culture studies revealed that both the material and the synthesis procedure are compatible with cells relevant to bone tissue engineering.
26836293	6	97	theme	Reliable	1194:1201	arg1	nucleation					1203:1212	Reliable nucleation	1194:1212	Reliable nucleation of a desired mineral phase within the alginate network	1194:1267	Reliable nucleation of a desired mineral phase within the alginate network proved more challenging than simple aqueous precipitation.
26836293	4	98	theme	study	839:843	arg1	focus					825:829	The main focus	816:829	The main focus of this study	816:843	The main focus of this study is to address these shortcomings by determining the precise fabrication parameters needed to prepare composites with controlled composition and properties.
26836293	8	99	theme	seeds	1730:1734	arg1	amount					1740:1745	amount	1740:1745	amount of precursor concentration	1740:1772	To overcome this, the incorporation of brushite seed crystals resulted in good control during the mineral phase, and by adjusting the number of seeds and amount of precursor concentration, the amount of mineral could be tuned.
26836293	8	99	theme	seeds	1730:1734	arg1	concentration					1760:1772	precursor concentration	1750:1772	precursor concentration	1750:1772	To overcome this, the incorporation of brushite seed crystals resulted in good control during the mineral phase, and by adjusting the number of seeds and amount of precursor concentration, the amount of mineral could be tuned.
26836293	8	99	theme	seeds	1730:1734	arg1	seeds					1730:1734	seeds	1730:1734	seeds	1730:1734	To overcome this, the incorporation of brushite seed crystals resulted in good control during the mineral phase, and by adjusting the number of seeds and amount of precursor concentration, the amount of mineral could be tuned.
26836293	8	99	theme	seeds	1730:1734	arg1	number					1720:1725	the number	1716:1725	the number of seeds	1716:1734	To overcome this, the incorporation of brushite seed crystals resulted in good control during the mineral phase, and by adjusting the number of seeds and amount of precursor concentration, the amount of mineral could be tuned.
26836293	2	100	from	use	410:412	arg1	combination					429:439	combination	429:439	combination with hydrogels	429:454	The use of brushite in combination with hydrogels opens up possibilities for new cell-based tissue engineering applications of this promising material.
26836293	9	101	theme	x-ray	1928:1932	arg1	diffraction					1934:1944	powder x-ray diffraction	1921:1944	powder x-ray diffraction	1921:1944	The material was characterised with a range of physical techniques, including scanning electron microscopy, powder x-ray diffraction and Rietveld refinement, Fourier transform infrared spectroscopy, and thermogravimetric analysis, in order to assess the mineral morphology, phase and amount within the organic matrix.
26836293	5	102	dep	hydrogel	1136:1143	arg1	the					1132:1134	the	1132:1134	the	1132:1134	Composite alginate microbeads were prepared using a counter-diffusion technique, which allows for the simultaneous crosslinking of the hydrogel and precipitation of an inorganic mineral phase.
26836293	4	103	theme	main	820:823	arg1	focus					825:829	The main focus	816:829	The main focus of this study	816:843	The main focus of this study is to address these shortcomings by determining the precise fabrication parameters needed to prepare composites with controlled composition and properties.
26836293	8	104	theme	precursor	1750:1758	arg1	concentration					1760:1772	precursor concentration	1750:1772	precursor concentration	1750:1772	To overcome this, the incorporation of brushite seed crystals resulted in good control during the mineral phase, and by adjusting the number of seeds and amount of precursor concentration, the amount of mineral could be tuned.
25965503	5	0	theme	xylan	629:633	arg1	fragments					635:643	short xylan fragments	623:643	short xylan fragments	623:643	A similar agreement was also found when short xylan fragments were adsorbed on the (110) surface of crystalline cellulose.
25965503	3	1	theme	short	342:346	arg1	fragments					354:362	short xylan fragments	342:362	short xylan fragments	342:362	Our calculations rely on force-field parameters adapted from the atomistic description of short xylan fragments and their adsorption on cellulose.
25965503	4	2	dep	good	488:491	arg1	match					493:497	match	493:497	match	493:497	This CG model was first validated for xylan chains both isolated and in the bulk where a good match was found with its atomistic counterpart as well as with experimental measurements.
25965503	5	3	theme	short	623:627	arg1	fragments					635:643	short xylan fragments	623:643	short xylan fragments	623:643	A similar agreement was also found when short xylan fragments were adsorbed on the (110) surface of crystalline cellulose.
25965503	6	4	theme	coiled	883:888	arg1	structures					890:899	coiled structures	883:899	coiled structures	883:899	The CG model, which was extended to the (100) and (1-10) surfaces, revealed that the adsorbed xylan, which was essentially extended in the atomistic situation, could also adopt coiled structures, especially when laying on the hydrophobic cellulose surfaces.
25965503	3	5	theme	xylan	348:352	arg1	fragments					354:362	short xylan fragments	342:362	short xylan fragments	342:362	Our calculations rely on force-field parameters adapted from the atomistic description of short xylan fragments and their adsorption on cellulose.
25965503	1	6	with	xylan	124:128	arg1	surfaces					177:184	crystalline cellulose surfaces	155:184	crystalline cellulose surfaces	155:184	We have built a coarse-grain (CG) model describing xylan and its interaction with crystalline cellulose surfaces.
25965503	4	7	dep	validated	423:431	arg1	both					450:453	both	450:453	both	450:453	This CG model was first validated for xylan chains both isolated and in the bulk where a good match was found with its atomistic counterpart as well as with experimental measurements.
25965503	5	8	theme	crystalline	683:693	arg1	cellulose					695:703	crystalline cellulose	683:703	crystalline cellulose	683:703	A similar agreement was also found when short xylan fragments were adsorbed on the (110) surface of crystalline cellulose.
25965503	6	9	theme	adsorbed	791:798	arg1	xylan					800:804	the adsorbed xylan	787:804	the adsorbed xylan	787:804	The CG model, which was extended to the (100) and (1-10) surfaces, revealed that the adsorbed xylan, which was essentially extended in the atomistic situation, could also adopt coiled structures, especially when laying on the hydrophobic cellulose surfaces.
25965503	5	10	theme	similar	585:591	arg1	agreement					593:601	A similar agreement	583:601	A similar agreement	583:601	A similar agreement was also found when short xylan fragments were adsorbed on the (110) surface of crystalline cellulose.
25965503	5	11	theme	cellulose	695:703	arg1	surface					672:678	the (110) surface	662:678	the (110) surface of crystalline cellulose	662:703	A similar agreement was also found when short xylan fragments were adsorbed on the (110) surface of crystalline cellulose.
25965503	0	12	theme	coarse-grain	2:13	arg1	force-field					15:25	A coarse-grain force-field	0:25	A coarse-grain force-field for xylan	0:35	A coarse-grain force-field for xylan and its interaction with cellulose.
25965503	3	13	from	adsorption	374:383	arg1	cellulose					388:396	cellulose	388:396	cellulose	388:396	Our calculations rely on force-field parameters adapted from the atomistic description of short xylan fragments and their adsorption on cellulose.
25965503	1	14	theme	crystalline	155:165	arg1	surfaces					177:184	crystalline cellulose surfaces	155:184	crystalline cellulose surfaces	155:184	We have built a coarse-grain (CG) model describing xylan and its interaction with crystalline cellulose surfaces.
25965503	1	15	theme	cellulose	167:175	arg1	surfaces					177:184	crystalline cellulose surfaces	155:184	crystalline cellulose surfaces	155:184	We have built a coarse-grain (CG) model describing xylan and its interaction with crystalline cellulose surfaces.
25965503	3	16	theme	fragments	354:362	arg1	adsorption					374:383	their adsorption	368:383	their adsorption on cellulose	368:396	Our calculations rely on force-field parameters adapted from the atomistic description of short xylan fragments and their adsorption on cellulose.
25965503	3	16	theme	fragments	354:362	arg1	description					327:337	the atomistic description	313:337	the atomistic description of short xylan fragments	313:362	Our calculations rely on force-field parameters adapted from the atomistic description of short xylan fragments and their adsorption on cellulose.
25965503	3	17	from	description	327:337	arg1	cellulose					388:396	cellulose	388:396	cellulose	388:396	Our calculations rely on force-field parameters adapted from the atomistic description of short xylan fragments and their adsorption on cellulose.
25965503	1	18	theme	coarse-grain	89:100	arg1	model					107:111	a coarse-grain (CG) model	87:111	a coarse-grain (CG) model describing xylan and its interaction with crystalline cellulose surfaces	87:184	We have built a coarse-grain (CG) model describing xylan and its interaction with crystalline cellulose surfaces.
25965503	6	19	theme	cellulose	944:952	arg1	surfaces					954:961	the hydrophobic cellulose surfaces	928:961	the hydrophobic cellulose surfaces	928:961	The CG model, which was extended to the (100) and (1-10) surfaces, revealed that the adsorbed xylan, which was essentially extended in the atomistic situation, could also adopt coiled structures, especially when laying on the hydrophobic cellulose surfaces.
25965503	4	20	theme	atomistic	518:526	arg1	counterpart					528:538	its atomistic counterpart	514:538	its atomistic counterpart	514:538	This CG model was first validated for xylan chains both isolated and in the bulk where a good match was found with its atomistic counterpart as well as with experimental measurements.
25965503	6	21	theme	hydrophobic	932:942	arg1	surfaces					954:961	the hydrophobic cellulose surfaces	928:961	the hydrophobic cellulose surfaces	928:961	The CG model, which was extended to the (100) and (1-10) surfaces, revealed that the adsorbed xylan, which was essentially extended in the atomistic situation, could also adopt coiled structures, especially when laying on the hydrophobic cellulose surfaces.
25965503	6	22	theme	atomistic	845:853	arg1	situation					855:863	the atomistic situation	841:863	the atomistic situation	841:863	The CG model, which was extended to the (100) and (1-10) surfaces, revealed that the adsorbed xylan, which was essentially extended in the atomistic situation, could also adopt coiled structures, especially when laying on the hydrophobic cellulose surfaces.
25965503	3	23	theme	force-field	277:287	arg1	parameters					289:298	force-field parameters	277:298	force-field parameters adapted from the atomistic description of short xylan fragments and their adsorption on cellulose	277:396	Our calculations rely on force-field parameters adapted from the atomistic description of short xylan fragments and their adsorption on cellulose.
25965503	4	24	theme	xylan	437:441	arg1	chains					443:448	xylan chains	437:448	xylan chains	437:448	This CG model was first validated for xylan chains both isolated and in the bulk where a good match was found with its atomistic counterpart as well as with experimental measurements.
25965503	2	25	theme	glucosyl	203:210	arg1	unit					212:215	Each xylosyl or glucosyl unit	187:215	unit	212:215	Each xylosyl or glucosyl unit was represented by a single grain.
25965503	0	26	with	force-field	15:25	arg1	cellulose					62:70	cellulose	62:70	cellulose	62:70	A coarse-grain force-field for xylan and its interaction with cellulose.
25965503	2	27	theme	xylosyl	192:198	arg1	unit					212:215	Each xylosyl or glucosyl unit	187:215	unit	212:215	Each xylosyl or glucosyl unit was represented by a single grain.
25965503	1	28	theme	CG	103:104	arg1	model					107:111	a coarse-grain (CG) model	87:111	a coarse-grain (CG) model describing xylan and its interaction with crystalline cellulose surfaces	87:184	We have built a coarse-grain (CG) model describing xylan and its interaction with crystalline cellulose surfaces.
25965503	1	29	with	interaction	138:148	arg1	surfaces					177:184	crystalline cellulose surfaces	155:184	crystalline cellulose surfaces	155:184	We have built a coarse-grain (CG) model describing xylan and its interaction with crystalline cellulose surfaces.
25965503	4	30	theme	CG	404:405	arg1	model					407:411	This CG model	399:411	This CG model	399:411	This CG model was first validated for xylan chains both isolated and in the bulk where a good match was found with its atomistic counterpart as well as with experimental measurements.
25965503	2	31	theme	single	238:243	arg1	grain					245:249	a single grain	236:249	a single grain	236:249	Each xylosyl or glucosyl unit was represented by a single grain.
25965503	6	32	theme	CG	710:711	arg1	model					713:717	The CG model	706:717	The CG model	706:717	The CG model, which was extended to the (100) and (1-10) surfaces, revealed that the adsorbed xylan, which was essentially extended in the atomistic situation, could also adopt coiled structures, especially when laying on the hydrophobic cellulose surfaces.
25965503	0	33	with	interaction	45:55	arg1	cellulose					62:70	cellulose	62:70	cellulose	62:70	A coarse-grain force-field for xylan and its interaction with cellulose.
25965503	4	34	theme	experimental	556:567	arg1	measurements					569:580	experimental measurements	556:580	experimental measurements	556:580	This CG model was first validated for xylan chains both isolated and in the bulk where a good match was found with its atomistic counterpart as well as with experimental measurements.
25965503	3	35	theme	atomistic	317:325	arg1	description					327:337	the atomistic description	313:337	the atomistic description of short xylan fragments	313:362	Our calculations rely on force-field parameters adapted from the atomistic description of short xylan fragments and their adsorption on cellulose.
24299789	6	0	theme	Confocal	869:876	arg1	CLSM					905:908	CLSM	905:908	CLSM	905:908	Confocal laser scanning microscope (CLSM) further confirmed the existence of an integrated chitosan film between beeswax layer and paper base and a thin composite layer consisting of chitosan and beeswax.
24299789	6	0	theme	Confocal	869:876	arg1	microscope					893:902	Confocal laser scanning microscope	869:902	Confocal laser scanning microscope (CLSM)	869:909	Confocal laser scanning microscope (CLSM) further confirmed the existence of an integrated chitosan film between beeswax layer and paper base and a thin composite layer consisting of chitosan and beeswax.
24299789	3	1	dep	/d	588:589	arg1	2					586:586	2	586:586	2	586:586	It was observed that as the concentration of chitosan solution increased from 1.0 to 3.0 wt%, its water vapour transport rate (WVTR) decreased from 171.6 to 52.8 g/m(2)/d but using reduced beeswax coating weight (from 10.1 to 4.9 g/m(2)).
24299789	1	2	theme	single	144:149	arg1	coating					157:163	single layer coating	144:163	single layer coating	144:163	In order to overcome the deficiencies of single layer coating, bilayer coated papers were prepared by two separate coating procedures using various combinations of proteins or polysaccharides with beeswax.
24299789	6	3	theme	chitosan	960:967	arg1	film					969:972	an integrated chitosan film	946:972	an integrated chitosan film between beeswax layer and paper base and a thin composite layer consisting of chitosan and beeswax	946:1071	Confocal laser scanning microscope (CLSM) further confirmed the existence of an integrated chitosan film between beeswax layer and paper base and a thin composite layer consisting of chitosan and beeswax.
24299789	5	4	theme	chitosan	798:805	arg1	layer					807:811	chitosan layer	798:811	chitosan layer	798:811	Scanning electron microscopy (SEM) showed that beeswax layer was fitted to chitosan layer so closely that these two layers are indistinguishable.
24299789	1	5	theme	layer	151:155	arg1	coating					157:163	single layer coating	144:163	single layer coating	144:163	In order to overcome the deficiencies of single layer coating, bilayer coated papers were prepared by two separate coating procedures using various combinations of proteins or polysaccharides with beeswax.
24299789	1	6	theme	various	243:249	arg1	combinations					251:262	various combinations	243:262	various combinations of proteins or polysaccharides with beeswax	243:306	In order to overcome the deficiencies of single layer coating, bilayer coated papers were prepared by two separate coating procedures using various combinations of proteins or polysaccharides with beeswax.
24299789	3	7	theme	vapour	524:529	arg1	WVTR					547:550	WVTR	547:550	WVTR	547:550	It was observed that as the concentration of chitosan solution increased from 1.0 to 3.0 wt%, its water vapour transport rate (WVTR) decreased from 171.6 to 52.8 g/m(2)/d but using reduced beeswax coating weight (from 10.1 to 4.9 g/m(2)).
24299789	3	7	theme	vapour	524:529	arg1	rate					541:544	its water vapour transport rate	514:544	its water vapour transport rate (WVTR)	514:551	It was observed that as the concentration of chitosan solution increased from 1.0 to 3.0 wt%, its water vapour transport rate (WVTR) decreased from 171.6 to 52.8 g/m(2)/d but using reduced beeswax coating weight (from 10.1 to 4.9 g/m(2)).
24299789	3	8	theme	transport	531:539	arg1	WVTR					547:550	WVTR	547:550	WVTR	547:550	It was observed that as the concentration of chitosan solution increased from 1.0 to 3.0 wt%, its water vapour transport rate (WVTR) decreased from 171.6 to 52.8 g/m(2)/d but using reduced beeswax coating weight (from 10.1 to 4.9 g/m(2)).
24299789	3	8	theme	transport	531:539	arg1	rate					541:544	its water vapour transport rate	514:544	its water vapour transport rate (WVTR)	514:551	It was observed that as the concentration of chitosan solution increased from 1.0 to 3.0 wt%, its water vapour transport rate (WVTR) decreased from 171.6 to 52.8 g/m(2)/d but using reduced beeswax coating weight (from 10.1 to 4.9 g/m(2)).
24299789	6	9	theme	integrated	949:958	arg1	film					969:972	an integrated chitosan film	946:972	an integrated chitosan film between beeswax layer and paper base and a thin composite layer consisting of chitosan and beeswax	946:1071	Confocal laser scanning microscope (CLSM) further confirmed the existence of an integrated chitosan film between beeswax layer and paper base and a thin composite layer consisting of chitosan and beeswax.
24299789	4	10	theme	enhanced	680:687	arg1	performance					689:699	an enhanced performance	677:699	an enhanced performance of grease resistance	677:720	It also displayed an enhanced performance of grease resistance.
24299789	1	11	theme	bilayer	166:172	arg1	papers					181:186	bilayer coated papers	166:186	bilayer coated papers	166:186	In order to overcome the deficiencies of single layer coating, bilayer coated papers were prepared by two separate coating procedures using various combinations of proteins or polysaccharides with beeswax.
24299789	1	12	theme	coating	157:163	arg1	deficiencies					128:139	the deficiencies	124:139	the deficiencies of single layer coating	124:163	In order to overcome the deficiencies of single layer coating, bilayer coated papers were prepared by two separate coating procedures using various combinations of proteins or polysaccharides with beeswax.
24299789	1	13	with	polysaccharides	279:293	arg1	beeswax					300:306	beeswax	300:306	beeswax	300:306	In order to overcome the deficiencies of single layer coating, bilayer coated papers were prepared by two separate coating procedures using various combinations of proteins or polysaccharides with beeswax.
24299789	3	14	theme	water	518:522	arg1	WVTR					547:550	WVTR	547:550	WVTR	547:550	It was observed that as the concentration of chitosan solution increased from 1.0 to 3.0 wt%, its water vapour transport rate (WVTR) decreased from 171.6 to 52.8 g/m(2)/d but using reduced beeswax coating weight (from 10.1 to 4.9 g/m(2)).
24299789	3	14	theme	water	518:522	arg1	rate					541:544	its water vapour transport rate	514:544	its water vapour transport rate (WVTR)	514:551	It was observed that as the concentration of chitosan solution increased from 1.0 to 3.0 wt%, its water vapour transport rate (WVTR) decreased from 171.6 to 52.8 g/m(2)/d but using reduced beeswax coating weight (from 10.1 to 4.9 g/m(2)).
24299789	2	15	theme	vapour	395:400	arg1	property					410:417	the best water vapour barrier property	380:417	the best water vapour barrier property	380:417	Among those combinations, chitosan-beeswax bilayer coated paper showed the best water vapour barrier property.
24299789	0	16	theme	water	9:13	arg1	barrier					22:28	Enhanced water vapour barrier	0:28	Enhanced water vapour barrier	0:28	Enhanced water vapour barrier and grease resistance of paper bilayer-coated with chitosan and beeswax.
24299789	6	17	theme	scanning	884:891	arg1	CLSM					905:908	CLSM	905:908	CLSM	905:908	Confocal laser scanning microscope (CLSM) further confirmed the existence of an integrated chitosan film between beeswax layer and paper base and a thin composite layer consisting of chitosan and beeswax.
24299789	6	17	theme	scanning	884:891	arg1	microscope					893:902	Confocal laser scanning microscope	869:902	Confocal laser scanning microscope (CLSM)	869:909	Confocal laser scanning microscope (CLSM) further confirmed the existence of an integrated chitosan film between beeswax layer and paper base and a thin composite layer consisting of chitosan and beeswax.
24299789	3	18	dep	3.0	505:507	arg1	to					502:503	to	502:503	to	502:503	It was observed that as the concentration of chitosan solution increased from 1.0 to 3.0 wt%, its water vapour transport rate (WVTR) decreased from 171.6 to 52.8 g/m(2)/d but using reduced beeswax coating weight (from 10.1 to 4.9 g/m(2)).
24299789	2	19	theme	water	389:393	arg1	property					410:417	the best water vapour barrier property	380:417	the best water vapour barrier property	380:417	Among those combinations, chitosan-beeswax bilayer coated paper showed the best water vapour barrier property.
24299789	0	20	theme	Enhanced	0:7	arg1	barrier					22:28	Enhanced water vapour barrier	0:28	Enhanced water vapour barrier	0:28	Enhanced water vapour barrier and grease resistance of paper bilayer-coated with chitosan and beeswax.
24299789	1	21	theme	proteins	267:274	arg1	combinations					251:262	various combinations	243:262	various combinations of proteins or polysaccharides with beeswax	243:306	In order to overcome the deficiencies of single layer coating, bilayer coated papers were prepared by two separate coating procedures using various combinations of proteins or polysaccharides with beeswax.
24299789	1	22	with	proteins	267:274	arg1	beeswax					300:306	beeswax	300:306	beeswax	300:306	In order to overcome the deficiencies of single layer coating, bilayer coated papers were prepared by two separate coating procedures using various combinations of proteins or polysaccharides with beeswax.
24299789	1	23	theme	coated	174:179	arg1	papers					181:186	bilayer coated papers	166:186	bilayer coated papers	166:186	In order to overcome the deficiencies of single layer coating, bilayer coated papers were prepared by two separate coating procedures using various combinations of proteins or polysaccharides with beeswax.
24299789	5	24	theme	electron	732:739	arg1	SEM					753:755	SEM	753:755	SEM	753:755	Scanning electron microscopy (SEM) showed that beeswax layer was fitted to chitosan layer so closely that these two layers are indistinguishable.
24299789	5	24	theme	electron	732:739	arg1	microscopy					741:750	Scanning electron microscopy	723:750	Scanning electron microscopy (SEM)	723:756	Scanning electron microscopy (SEM) showed that beeswax layer was fitted to chitosan layer so closely that these two layers are indistinguishable.
24299789	5	25	theme	Scanning	723:730	arg1	SEM					753:755	SEM	753:755	SEM	753:755	Scanning electron microscopy (SEM) showed that beeswax layer was fitted to chitosan layer so closely that these two layers are indistinguishable.
24299789	5	25	theme	Scanning	723:730	arg1	microscopy					741:750	Scanning electron microscopy	723:750	Scanning electron microscopy (SEM)	723:756	Scanning electron microscopy (SEM) showed that beeswax layer was fitted to chitosan layer so closely that these two layers are indistinguishable.
24299789	6	26	dep	layer	990:994	arg1	base					1006:1009	base	1006:1009	base	1006:1009	Confocal laser scanning microscope (CLSM) further confirmed the existence of an integrated chitosan film between beeswax layer and paper base and a thin composite layer consisting of chitosan and beeswax.
24299789	6	27	theme	beeswax	982:988	arg1	layer					990:994	beeswax layer	982:994	beeswax layer	982:994	Confocal laser scanning microscope (CLSM) further confirmed the existence of an integrated chitosan film between beeswax layer and paper base and a thin composite layer consisting of chitosan and beeswax.
24299789	3	28	theme	chitosan	465:472	arg1	solution					474:481	chitosan solution	465:481	chitosan solution	465:481	It was observed that as the concentration of chitosan solution increased from 1.0 to 3.0 wt%, its water vapour transport rate (WVTR) decreased from 171.6 to 52.8 g/m(2)/d but using reduced beeswax coating weight (from 10.1 to 4.9 g/m(2)).
24299789	0	29	theme	vapour	15:20	arg1	barrier					22:28	Enhanced water vapour barrier	0:28	Enhanced water vapour barrier	0:28	Enhanced water vapour barrier and grease resistance of paper bilayer-coated with chitosan and beeswax.
24299789	1	30	theme	polysaccharides	279:293	arg1	combinations					251:262	various combinations	243:262	various combinations of proteins or polysaccharides with beeswax	243:306	In order to overcome the deficiencies of single layer coating, bilayer coated papers were prepared by two separate coating procedures using various combinations of proteins or polysaccharides with beeswax.
24299789	2	31	theme	coated	360:365	arg1	paper					367:371	chitosan-beeswax bilayer coated paper	335:371	chitosan-beeswax bilayer coated paper	335:371	Among those combinations, chitosan-beeswax bilayer coated paper showed the best water vapour barrier property.
24299789	3	32	theme	solution	474:481	arg1	concentration					448:460	the concentration	444:460	the concentration of chitosan solution	444:481	It was observed that as the concentration of chitosan solution increased from 1.0 to 3.0 wt%, its water vapour transport rate (WVTR) decreased from 171.6 to 52.8 g/m(2)/d but using reduced beeswax coating weight (from 10.1 to 4.9 g/m(2)).
24299789	0	33	theme	grease	34:39	arg1	resistance					41:50	grease resistance	34:50	grease resistance of paper	34:59	Enhanced water vapour barrier and grease resistance of paper bilayer-coated with chitosan and beeswax.
24299789	6	34	theme	composite	1022:1030	arg1	layer					1032:1036	a thin composite layer	1015:1036	a thin composite layer consisting of chitosan and beeswax	1015:1071	Confocal laser scanning microscope (CLSM) further confirmed the existence of an integrated chitosan film between beeswax layer and paper base and a thin composite layer consisting of chitosan and beeswax.
24299789	2	35	theme	best	384:387	arg1	property					410:417	the best water vapour barrier property	380:417	the best water vapour barrier property	380:417	Among those combinations, chitosan-beeswax bilayer coated paper showed the best water vapour barrier property.
24299789	2	36	theme	barrier	402:408	arg1	property					410:417	the best water vapour barrier property	380:417	the best water vapour barrier property	380:417	Among those combinations, chitosan-beeswax bilayer coated paper showed the best water vapour barrier property.
24299789	3	37	dep	4.9	646:648	arg1	to					643:644	to	643:644	to	643:644	It was observed that as the concentration of chitosan solution increased from 1.0 to 3.0 wt%, its water vapour transport rate (WVTR) decreased from 171.6 to 52.8 g/m(2)/d but using reduced beeswax coating weight (from 10.1 to 4.9 g/m(2)).
24299789	6	38	theme	laser	878:882	arg1	CLSM					905:908	CLSM	905:908	CLSM	905:908	Confocal laser scanning microscope (CLSM) further confirmed the existence of an integrated chitosan film between beeswax layer and paper base and a thin composite layer consisting of chitosan and beeswax.
24299789	6	38	theme	laser	878:882	arg1	microscope					893:902	Confocal laser scanning microscope	869:902	Confocal laser scanning microscope (CLSM)	869:909	Confocal laser scanning microscope (CLSM) further confirmed the existence of an integrated chitosan film between beeswax layer and paper base and a thin composite layer consisting of chitosan and beeswax.
24299789	2	39	theme	bilayer	352:358	arg1	paper					367:371	chitosan-beeswax bilayer coated paper	335:371	chitosan-beeswax bilayer coated paper	335:371	Among those combinations, chitosan-beeswax bilayer coated paper showed the best water vapour barrier property.
24299789	6	40	theme	film	969:972	arg1	existence					933:941	the existence	929:941	the existence of an integrated chitosan film between beeswax layer and paper base and a thin composite layer consisting of chitosan and beeswax	929:1071	Confocal laser scanning microscope (CLSM) further confirmed the existence of an integrated chitosan film between beeswax layer and paper base and a thin composite layer consisting of chitosan and beeswax.
24299789	3	41	theme	coating	617:623	arg1	g/m					650:652	from 10.1 to 4.9 g/m(2)	633:655	from 10.1 to 4.9 g/m(2)	633:655	It was observed that as the concentration of chitosan solution increased from 1.0 to 3.0 wt%, its water vapour transport rate (WVTR) decreased from 171.6 to 52.8 g/m(2)/d but using reduced beeswax coating weight (from 10.1 to 4.9 g/m(2)).
24299789	3	41	theme	coating	617:623	arg1	weight					625:630	reduced beeswax coating weight	601:630	reduced beeswax coating weight (from 10.1 to 4.9 g/m(2))	601:656	It was observed that as the concentration of chitosan solution increased from 1.0 to 3.0 wt%, its water vapour transport rate (WVTR) decreased from 171.6 to 52.8 g/m(2)/d but using reduced beeswax coating weight (from 10.1 to 4.9 g/m(2)).
24299789	3	42	theme	wt	509:510	arg1	%					511:511	1.0 to 3.0 wt%	498:511	1.0 to 3.0 wt%	498:511	It was observed that as the concentration of chitosan solution increased from 1.0 to 3.0 wt%, its water vapour transport rate (WVTR) decreased from 171.6 to 52.8 g/m(2)/d but using reduced beeswax coating weight (from 10.1 to 4.9 g/m(2)).
24299789	2	43	theme	chitosan-beeswax	335:350	arg1	paper					367:371	chitosan-beeswax bilayer coated paper	335:371	chitosan-beeswax bilayer coated paper	335:371	Among those combinations, chitosan-beeswax bilayer coated paper showed the best water vapour barrier property.
24299789	4	44	theme	grease	704:709	arg1	resistance					711:720	grease resistance	704:720	grease resistance	704:720	It also displayed an enhanced performance of grease resistance.
24299789	6	45	theme	thin	1017:1020	arg1	layer					1032:1036	a thin composite layer	1015:1036	a thin composite layer consisting of chitosan and beeswax	1015:1071	Confocal laser scanning microscope (CLSM) further confirmed the existence of an integrated chitosan film between beeswax layer and paper base and a thin composite layer consisting of chitosan and beeswax.
24299789	3	46	theme	reduced	601:607	arg1	g/m					650:652	from 10.1 to 4.9 g/m(2)	633:655	from 10.1 to 4.9 g/m(2)	633:655	It was observed that as the concentration of chitosan solution increased from 1.0 to 3.0 wt%, its water vapour transport rate (WVTR) decreased from 171.6 to 52.8 g/m(2)/d but using reduced beeswax coating weight (from 10.1 to 4.9 g/m(2)).
24299789	3	46	theme	reduced	601:607	arg1	weight					625:630	reduced beeswax coating weight	601:630	reduced beeswax coating weight (from 10.1 to 4.9 g/m(2))	601:656	It was observed that as the concentration of chitosan solution increased from 1.0 to 3.0 wt%, its water vapour transport rate (WVTR) decreased from 171.6 to 52.8 g/m(2)/d but using reduced beeswax coating weight (from 10.1 to 4.9 g/m(2)).
24299789	3	47	dep	increased	483:491	arg1	as					441:442	as	441:442	as	441:442	It was observed that as the concentration of chitosan solution increased from 1.0 to 3.0 wt%, its water vapour transport rate (WVTR) decreased from 171.6 to 52.8 g/m(2)/d but using reduced beeswax coating weight (from 10.1 to 4.9 g/m(2)).
24299789	1	48	theme	separate	209:216	arg1	procedures					226:235	two separate coating procedures	205:235	two separate coating procedures using various combinations of proteins or polysaccharides with beeswax	205:306	In order to overcome the deficiencies of single layer coating, bilayer coated papers were prepared by two separate coating procedures using various combinations of proteins or polysaccharides with beeswax.
24299789	3	49	theme	beeswax	609:615	arg1	g/m					650:652	from 10.1 to 4.9 g/m(2)	633:655	from 10.1 to 4.9 g/m(2)	633:655	It was observed that as the concentration of chitosan solution increased from 1.0 to 3.0 wt%, its water vapour transport rate (WVTR) decreased from 171.6 to 52.8 g/m(2)/d but using reduced beeswax coating weight (from 10.1 to 4.9 g/m(2)).
24299789	3	49	theme	beeswax	609:615	arg1	weight					625:630	reduced beeswax coating weight	601:630	reduced beeswax coating weight (from 10.1 to 4.9 g/m(2))	601:656	It was observed that as the concentration of chitosan solution increased from 1.0 to 3.0 wt%, its water vapour transport rate (WVTR) decreased from 171.6 to 52.8 g/m(2)/d but using reduced beeswax coating weight (from 10.1 to 4.9 g/m(2)).
24299789	4	50	theme	resistance	711:720	arg1	performance					689:699	an enhanced performance	677:699	an enhanced performance of grease resistance	677:720	It also displayed an enhanced performance of grease resistance.
24299789	3	51	dep	52.8	577:580	arg1	to					574:575	to	574:575	to	574:575	It was observed that as the concentration of chitosan solution increased from 1.0 to 3.0 wt%, its water vapour transport rate (WVTR) decreased from 171.6 to 52.8 g/m(2)/d but using reduced beeswax coating weight (from 10.1 to 4.9 g/m(2)).
24299789	5	52	theme	beeswax	770:776	arg1	layer					778:782	beeswax layer	770:782	beeswax layer	770:782	Scanning electron microscopy (SEM) showed that beeswax layer was fitted to chitosan layer so closely that these two layers are indistinguishable.
24299789	1	53	theme	coating	218:224	arg1	procedures					226:235	two separate coating procedures	205:235	two separate coating procedures using various combinations of proteins or polysaccharides with beeswax	205:306	In order to overcome the deficiencies of single layer coating, bilayer coated papers were prepared by two separate coating procedures using various combinations of proteins or polysaccharides with beeswax.
24299789	0	54	theme	paper	55:59	arg1	barrier					22:28	Enhanced water vapour barrier	0:28	Enhanced water vapour barrier	0:28	Enhanced water vapour barrier and grease resistance of paper bilayer-coated with chitosan and beeswax.
24299789	0	54	theme	paper	55:59	arg1	resistance					41:50	grease resistance	34:50	grease resistance of paper	34:59	Enhanced water vapour barrier and grease resistance of paper bilayer-coated with chitosan and beeswax.
24299789	3	55	theme	g/m	582:584	arg1	/d					588:589	171.6 to 52.8 g/m(2)/d	568:589	171.6 to 52.8 g/m(2)/d	568:589	It was observed that as the concentration of chitosan solution increased from 1.0 to 3.0 wt%, its water vapour transport rate (WVTR) decreased from 171.6 to 52.8 g/m(2)/d but using reduced beeswax coating weight (from 10.1 to 4.9 g/m(2)).
28982096	0	0	theme	Cancer	88:93	arg1	Cells					95:99	Cancer Cells	88:99	Cancer Cells	88:99	Heparan Sulfate and Chondroitin Sulfate Glycosaminoglycans Are Targeted by Bleomycin in Cancer Cells.
28982096	10	1	theme	CONCLUSION	1459:1468	arg1	GAGs					1470:1473	CONCLUSION GAGs	1459:1473	CONCLUSION GAGs	1459:1473	CONCLUSION GAGs were targeted by bleomycin both at cell surface and at Golgi.
28982096	5	2	theme	GAG	645:647	arg1	biosynthesis					649:660	Golgi-operated GAG biosynthesis	630:660	Golgi-operated GAG biosynthesis	630:660	We therefore hypothesized that bleomycin might perturb Golgi-operated GAG biosynthesis.
28982096	7	3	used	used	905:908	arg2	we					902:903	we	902:903	we	902:903	To further understand the cytotoxicity of bleomycin and its relationship to GAGs, we used sodium chlorate to inhibit GAG sulfation and commercially available GAGs to compete for cell surface GAG/bleomycin interactions in seven cell lines including CHO745 defective in both heparan sulfate and chondroitin sulfate biosynthesis.
28982096	8	4	theme	concentration-	1326:1339	arg1	manner					1360:1365	a concentration- and time-dependent manner	1324:1365	a concentration- and time-dependent manner	1324:1365	RESULTS we discovered that heparan sulfate GAG was significantly undersulfated and the quantity and disaccharide compositions of GAGs were changed in bleomycin-treated cells in a concentration- and time-dependent manner.
28982096	7	5	theme	sodium	910:915	arg1	chlorate					917:924	sodium chlorate	910:924	sodium chlorate	910:924	To further understand the cytotoxicity of bleomycin and its relationship to GAGs, we used sodium chlorate to inhibit GAG sulfation and commercially available GAGs to compete for cell surface GAG/bleomycin interactions in seven cell lines including CHO745 defective in both heparan sulfate and chondroitin sulfate biosynthesis.
28982096	9	6	theme	surface	1445:1451	arg1	GAGs					1453:1456	cell surface GAGs	1440:1456	cell surface GAGs	1440:1456	We revealed that bleomycin-induced cytotoxicity was directly related to cell surface GAGs.
28982096	11	7	theme	severe	1677:1682	arg1	fibrosis					1638:1645	the bleomycin-induced fibrosis	1616:1645	the bleomycin-induced fibrosis in certain cancer patients	1616:1672	Thus, GAGs might be the biological relevant molecules that might be related to the bleomycin-induced fibrosis in certain cancer patients, a severe side effect with largely unknown molecular mechanism.
28982096	11	7	theme	severe	1677:1682	arg1	effect					1689:1694	a severe side effect	1675:1694	a severe side effect with largely unknown molecular mechanism	1675:1735	Thus, GAGs might be the biological relevant molecules that might be related to the bleomycin-induced fibrosis in certain cancer patients, a severe side effect with largely unknown molecular mechanism.
28982096	8	8	theme	bleomycin-treated	1297:1313	arg1	cells					1315:1319	bleomycin-treated cells	1297:1319	bleomycin-treated cells	1297:1319	RESULTS we discovered that heparan sulfate GAG was significantly undersulfated and the quantity and disaccharide compositions of GAGs were changed in bleomycin-treated cells in a concentration- and time-dependent manner.
28982096	7	9	theme	GAG/bleomycin	1011:1023	arg1	interactions					1025:1036	cell surface GAG/bleomycin interactions	998:1036	cell surface GAG/bleomycin interactions in seven cell lines including CHO745 defective in both heparan sulfate and chondroitin sulfate biosynthesis	998:1144	To further understand the cytotoxicity of bleomycin and its relationship to GAGs, we used sodium chlorate to inhibit GAG sulfation and commercially available GAGs to compete for cell surface GAG/bleomycin interactions in seven cell lines including CHO745 defective in both heparan sulfate and chondroitin sulfate biosynthesis.
28982096	3	10	theme	cell	347:350	arg1	glycosaminoglycans					379:396	the cell surface negatively charged glycosaminoglycans	343:396	the cell surface negatively charged glycosaminoglycans (GAGs)	343:403	We previously reported that the cell surface negatively charged glycosaminoglycans (GAGs) may be involved in the cellular uptake of bleomycin.
28982096	3	10	theme	cell	347:350	arg1	GAGs					399:402	GAGs	399:402	GAGs	399:402	We previously reported that the cell surface negatively charged glycosaminoglycans (GAGs) may be involved in the cellular uptake of bleomycin.
28982096	11	11	theme	certain	1650:1656	arg1	patients					1665:1672	certain cancer patients	1650:1672	certain cancer patients	1650:1672	Thus, GAGs might be the biological relevant molecules that might be related to the bleomycin-induced fibrosis in certain cancer patients, a severe side effect with largely unknown molecular mechanism.
28982096	7	12	theme	surface	1003:1009	arg1	interactions					1025:1036	cell surface GAG/bleomycin interactions	998:1036	cell surface GAG/bleomycin interactions in seven cell lines including CHO745 defective in both heparan sulfate and chondroitin sulfate biosynthesis	998:1144	To further understand the cytotoxicity of bleomycin and its relationship to GAGs, we used sodium chlorate to inhibit GAG sulfation and commercially available GAGs to compete for cell surface GAG/bleomycin interactions in seven cell lines including CHO745 defective in both heparan sulfate and chondroitin sulfate biosynthesis.
28982096	3	13	theme	surface	352:358	arg1	glycosaminoglycans					379:396	the cell surface negatively charged glycosaminoglycans	343:396	the cell surface negatively charged glycosaminoglycans (GAGs)	343:403	We previously reported that the cell surface negatively charged glycosaminoglycans (GAGs) may be involved in the cellular uptake of bleomycin.
28982096	3	13	theme	surface	352:358	arg1	GAGs					399:402	GAGs	399:402	GAGs	399:402	We previously reported that the cell surface negatively charged glycosaminoglycans (GAGs) may be involved in the cellular uptake of bleomycin.
28982096	6	14	used	used	674:677	arg2	We					671:672	We	671:672	We	671:672	METHODS We used stable isotope labeling coupled with LC/MS analysis of GAG disaccharides simultaneously from bleomycin-treated and non-treated cancer cells.
28982096	11	15	theme	side	1684:1687	arg1	fibrosis					1638:1645	the bleomycin-induced fibrosis	1616:1645	the bleomycin-induced fibrosis in certain cancer patients	1616:1672	Thus, GAGs might be the biological relevant molecules that might be related to the bleomycin-induced fibrosis in certain cancer patients, a severe side effect with largely unknown molecular mechanism.
28982096	11	15	theme	side	1684:1687	arg1	effect					1689:1694	a severe side effect	1675:1694	a severe side effect with largely unknown molecular mechanism	1675:1735	Thus, GAGs might be the biological relevant molecules that might be related to the bleomycin-induced fibrosis in certain cancer patients, a severe side effect with largely unknown molecular mechanism.
28982096	4	16	contain	has	526:528	arg1	class					482:486	a class	480:486	a class of positively charged small molecules	480:524	We also observed that a class of positively charged small molecules has Golgi localization once inside of the cells.
28982096	4	16	contain	has	526:528	arg2	localization					536:547	Golgi localization	530:547	Golgi localization	530:547	We also observed that a class of positively charged small molecules has Golgi localization once inside of the cells.
28982096	6	17	theme	LC/MS	716:720	arg1	analysis					722:729	LC/MS analysis	716:729	LC/MS analysis of GAG disaccharides simultaneously from bleomycin-treated and non-treated cancer cells	716:817	METHODS We used stable isotope labeling coupled with LC/MS analysis of GAG disaccharides simultaneously from bleomycin-treated and non-treated cancer cells.
28982096	4	18	theme	cells	568:572	arg1	inside					554:559	inside	554:559	inside of the cells	554:572	We also observed that a class of positively charged small molecules has Golgi localization once inside of the cells.
28982096	9	19	theme	bleomycin-induced	1385:1401	arg1	cytotoxicity					1403:1414	bleomycin-induced cytotoxicity	1385:1414	bleomycin-induced cytotoxicity	1385:1414	We revealed that bleomycin-induced cytotoxicity was directly related to cell surface GAGs.
28982096	1	20	theme	DNA	180:182	arg1	breaks					184:189	DNA breaks	180:189	DNA breaks	180:189	BACKGROUND/AIMS Bleomycin is a clinically used anti-cancer drug that produces DNA breaks once inside of cells.
28982096	10	21	theme	cell	1510:1513	arg1	surface					1515:1521	cell surface	1510:1521	cell surface	1510:1521	CONCLUSION GAGs were targeted by bleomycin both at cell surface and at Golgi.
28982096	8	22	theme	quantity	1234:1241	arg1	compositions					1260:1271	the quantity and disaccharide compositions	1230:1271	compositions	1260:1271	RESULTS we discovered that heparan sulfate GAG was significantly undersulfated and the quantity and disaccharide compositions of GAGs were changed in bleomycin-treated cells in a concentration- and time-dependent manner.
28982096	3	23	theme	cellular	428:435	arg1	uptake					437:442	the cellular uptake	424:442	the cellular uptake of bleomycin	424:455	We previously reported that the cell surface negatively charged glycosaminoglycans (GAGs) may be involved in the cellular uptake of bleomycin.
28982096	0	24	theme	Sulfate	8:14	arg1	Glycosaminoglycans					40:57	Heparan Sulfate and Chondroitin Sulfate Glycosaminoglycans	0:57	Heparan Sulfate and Chondroitin Sulfate Glycosaminoglycans	0:57	Heparan Sulfate and Chondroitin Sulfate Glycosaminoglycans Are Targeted by Bleomycin in Cancer Cells.
28982096	9	25	theme	cell	1440:1443	arg1	GAGs					1453:1456	cell surface GAGs	1440:1456	cell surface GAGs	1440:1456	We revealed that bleomycin-induced cytotoxicity was directly related to cell surface GAGs.
28982096	7	26	theme	chondroitin	1113:1123	arg1	sulfate					1125:1131	chondroitin sulfate	1113:1131	chondroitin sulfate	1113:1131	To further understand the cytotoxicity of bleomycin and its relationship to GAGs, we used sodium chlorate to inhibit GAG sulfation and commercially available GAGs to compete for cell surface GAG/bleomycin interactions in seven cell lines including CHO745 defective in both heparan sulfate and chondroitin sulfate biosynthesis.
28982096	7	27	theme	available	968:976	arg1	GAGs					978:981	commercially available GAGs	955:981	commercially available GAGs	955:981	To further understand the cytotoxicity of bleomycin and its relationship to GAGs, we used sodium chlorate to inhibit GAG sulfation and commercially available GAGs to compete for cell surface GAG/bleomycin interactions in seven cell lines including CHO745 defective in both heparan sulfate and chondroitin sulfate biosynthesis.
28982096	0	28	theme	Heparan	0:6	arg1	Sulfate					8:14	Heparan Sulfate	0:14	Heparan Sulfate	0:14	Heparan Sulfate and Chondroitin Sulfate Glycosaminoglycans Are Targeted by Bleomycin in Cancer Cells.
28982096	5	29	theme	Golgi-operated	630:643	arg1	biosynthesis					649:660	Golgi-operated GAG biosynthesis	630:660	Golgi-operated GAG biosynthesis	630:660	We therefore hypothesized that bleomycin might perturb Golgi-operated GAG biosynthesis.
28982096	6	30	theme	cancer	806:811	arg1	cells					813:817	bleomycin-treated and non-treated cancer cells	772:817	bleomycin-treated and non-treated cancer cells	772:817	METHODS We used stable isotope labeling coupled with LC/MS analysis of GAG disaccharides simultaneously from bleomycin-treated and non-treated cancer cells.
28982096	2	31	theme	free	299:302	arg1	diffusion					304:312	free diffusion	299:312	free diffusion	299:312	However, bleomycin is a positively charged molecule and cannot get inside of cells by free diffusion.
28982096	6	32	theme	isotope	686:692	arg1	labeling					694:701	stable isotope labeling	679:701	stable isotope labeling coupled with LC/MS analysis of GAG disaccharides simultaneously from bleomycin-treated and non-treated cancer cells	679:817	METHODS We used stable isotope labeling coupled with LC/MS analysis of GAG disaccharides simultaneously from bleomycin-treated and non-treated cancer cells.
28982096	7	33	from	interactions	1025:1036	arg1	CHO745					1068:1073	CHO745	1068:1073	CHO745 defective in both heparan sulfate and chondroitin sulfate biosynthesis	1068:1144	To further understand the cytotoxicity of bleomycin and its relationship to GAGs, we used sodium chlorate to inhibit GAG sulfation and commercially available GAGs to compete for cell surface GAG/bleomycin interactions in seven cell lines including CHO745 defective in both heparan sulfate and chondroitin sulfate biosynthesis.
28982096	7	33	from	interactions	1025:1036	arg1	lines					1052:1056	seven cell lines	1041:1056	seven cell lines including CHO745 defective in both heparan sulfate and chondroitin sulfate biosynthesis	1041:1144	To further understand the cytotoxicity of bleomycin and its relationship to GAGs, we used sodium chlorate to inhibit GAG sulfation and commercially available GAGs to compete for cell surface GAG/bleomycin interactions in seven cell lines including CHO745 defective in both heparan sulfate and chondroitin sulfate biosynthesis.
28982096	0	34	theme	Chondroitin	20:30	arg1	Sulfate					32:38	Chondroitin Sulfate	20:38	Chondroitin Sulfate	20:38	Heparan Sulfate and Chondroitin Sulfate Glycosaminoglycans Are Targeted by Bleomycin in Cancer Cells.
28982096	4	35	theme	Golgi	530:534	arg1	localization					536:547	Golgi localization	530:547	Golgi localization	530:547	We also observed that a class of positively charged small molecules has Golgi localization once inside of the cells.
28982096	7	36	theme	sulfate	1101:1107	arg1	biosynthesis					1133:1144	both heparan sulfate and chondroitin sulfate biosynthesis	1088:1144	both heparan sulfate and chondroitin sulfate biosynthesis	1088:1144	To further understand the cytotoxicity of bleomycin and its relationship to GAGs, we used sodium chlorate to inhibit GAG sulfation and commercially available GAGs to compete for cell surface GAG/bleomycin interactions in seven cell lines including CHO745 defective in both heparan sulfate and chondroitin sulfate biosynthesis.
28982096	6	37	theme	non-treated	794:804	arg1	cells					813:817	bleomycin-treated and non-treated cancer cells	772:817	bleomycin-treated and non-treated cancer cells	772:817	METHODS We used stable isotope labeling coupled with LC/MS analysis of GAG disaccharides simultaneously from bleomycin-treated and non-treated cancer cells.
28982096	1	38	theme	BACKGROUND/AIMS	102:116	arg1	drug					161:164	a clinically used anti-cancer drug	131:164	a clinically used anti-cancer drug that produces DNA breaks once inside of cells	131:210	BACKGROUND/AIMS Bleomycin is a clinically used anti-cancer drug that produces DNA breaks once inside of cells.
28982096	1	38	theme	BACKGROUND/AIMS	102:116	arg1	Bleomycin					118:126	BACKGROUND/AIMS Bleomycin	102:126	BACKGROUND/AIMS Bleomycin	102:126	BACKGROUND/AIMS Bleomycin is a clinically used anti-cancer drug that produces DNA breaks once inside of cells.
28982096	6	39	theme	stable	679:684	arg1	labeling					694:701	stable isotope labeling	679:701	stable isotope labeling coupled with LC/MS analysis of GAG disaccharides simultaneously from bleomycin-treated and non-treated cancer cells	679:817	METHODS We used stable isotope labeling coupled with LC/MS analysis of GAG disaccharides simultaneously from bleomycin-treated and non-treated cancer cells.
28982096	3	40	theme	bleomycin	447:455	arg1	uptake					437:442	the cellular uptake	424:442	the cellular uptake of bleomycin	424:455	We previously reported that the cell surface negatively charged glycosaminoglycans (GAGs) may be involved in the cellular uptake of bleomycin.
28982096	3	41	theme	charged	371:377	arg1	glycosaminoglycans					379:396	the cell surface negatively charged glycosaminoglycans	343:396	the cell surface negatively charged glycosaminoglycans (GAGs)	343:403	We previously reported that the cell surface negatively charged glycosaminoglycans (GAGs) may be involved in the cellular uptake of bleomycin.
28982096	3	41	theme	charged	371:377	arg1	GAGs					399:402	GAGs	399:402	GAGs	399:402	We previously reported that the cell surface negatively charged glycosaminoglycans (GAGs) may be involved in the cellular uptake of bleomycin.
28982096	7	42	from	biosynthesis	1133:1144	arg1	defective					1075:1083	defective	1075:1083	defective	1075:1083	To further understand the cytotoxicity of bleomycin and its relationship to GAGs, we used sodium chlorate to inhibit GAG sulfation and commercially available GAGs to compete for cell surface GAG/bleomycin interactions in seven cell lines including CHO745 defective in both heparan sulfate and chondroitin sulfate biosynthesis.
28982096	7	43	theme	cell	998:1001	arg1	interactions					1025:1036	cell surface GAG/bleomycin interactions	998:1036	cell surface GAG/bleomycin interactions in seven cell lines including CHO745 defective in both heparan sulfate and chondroitin sulfate biosynthesis	998:1144	To further understand the cytotoxicity of bleomycin and its relationship to GAGs, we used sodium chlorate to inhibit GAG sulfation and commercially available GAGs to compete for cell surface GAG/bleomycin interactions in seven cell lines including CHO745 defective in both heparan sulfate and chondroitin sulfate biosynthesis.
28982096	2	44	theme	cells	290:294	arg1	inside					280:285	inside	280:285	inside of cells by free diffusion	280:312	However, bleomycin is a positively charged molecule and cannot get inside of cells by free diffusion.
28982096	1	45	theme	cells	206:210	arg1	inside					196:201	inside	196:201	inside of cells	196:210	BACKGROUND/AIMS Bleomycin is a clinically used anti-cancer drug that produces DNA breaks once inside of cells.
28982096	8	46	theme	GAGs	1276:1279	arg1	compositions					1260:1271	the quantity and disaccharide compositions	1230:1271	compositions	1260:1271	RESULTS we discovered that heparan sulfate GAG was significantly undersulfated and the quantity and disaccharide compositions of GAGs were changed in bleomycin-treated cells in a concentration- and time-dependent manner.
28982096	7	47	theme	bleomycin	862:870	arg1	relationship					880:891	its relationship	876:891	its relationship to GAGs	876:899	To further understand the cytotoxicity of bleomycin and its relationship to GAGs, we used sodium chlorate to inhibit GAG sulfation and commercially available GAGs to compete for cell surface GAG/bleomycin interactions in seven cell lines including CHO745 defective in both heparan sulfate and chondroitin sulfate biosynthesis.
28982096	7	47	theme	bleomycin	862:870	arg1	cytotoxicity					846:857	the cytotoxicity	842:857	the cytotoxicity of bleomycin	842:870	To further understand the cytotoxicity of bleomycin and its relationship to GAGs, we used sodium chlorate to inhibit GAG sulfation and commercially available GAGs to compete for cell surface GAG/bleomycin interactions in seven cell lines including CHO745 defective in both heparan sulfate and chondroitin sulfate biosynthesis.
28982096	8	48	dep	RESULTS	1147:1153	arg1	discovered					1158:1167	discovered	1158:1167	discovered that heparan sulfate GAG was significantly undersulfated and the quantity and disaccharide compositions of GAGs were changed in bleomycin-treated cells in a concentration- and time-dependent manner	1158:1365	RESULTS we discovered that heparan sulfate GAG was significantly undersulfated and the quantity and disaccharide compositions of GAGs were changed in bleomycin-treated cells in a concentration- and time-dependent manner.
28982096	11	49	theme	relevant	1572:1579	arg1	molecules					1581:1589	the biological relevant molecules	1557:1589	the biological relevant molecules that might be related to the bleomycin-induced fibrosis in certain cancer patients, a severe side effect with largely unknown molecular mechanism	1557:1735	Thus, GAGs might be the biological relevant molecules that might be related to the bleomycin-induced fibrosis in certain cancer patients, a severe side effect with largely unknown molecular mechanism.
28982096	11	49	theme	relevant	1572:1579	arg1	related					1605:1611	related	1605:1611	related	1605:1611	Thus, GAGs might be the biological relevant molecules that might be related to the bleomycin-induced fibrosis in certain cancer patients, a severe side effect with largely unknown molecular mechanism.
28982096	11	49	theme	relevant	1572:1579	arg1	GAGs					1543:1546	GAGs	1543:1546	GAGs	1543:1546	Thus, GAGs might be the biological relevant molecules that might be related to the bleomycin-induced fibrosis in certain cancer patients, a severe side effect with largely unknown molecular mechanism.
28982096	8	50	theme	time-dependent	1345:1358	arg1	manner					1360:1365	a concentration- and time-dependent manner	1324:1365	a concentration- and time-dependent manner	1324:1365	RESULTS we discovered that heparan sulfate GAG was significantly undersulfated and the quantity and disaccharide compositions of GAGs were changed in bleomycin-treated cells in a concentration- and time-dependent manner.
28982096	0	51	theme	Sulfate	32:38	arg1	Glycosaminoglycans					40:57	Heparan Sulfate and Chondroitin Sulfate Glycosaminoglycans	0:57	Heparan Sulfate and Chondroitin Sulfate Glycosaminoglycans	0:57	Heparan Sulfate and Chondroitin Sulfate Glycosaminoglycans Are Targeted by Bleomycin in Cancer Cells.
28982096	4	52	theme	small	510:514	arg1	molecules					516:524	positively charged small molecules	491:524	positively charged small molecules	491:524	We also observed that a class of positively charged small molecules has Golgi localization once inside of the cells.
28982096	7	53	theme	heparan	1093:1099	arg1	sulfate					1101:1107	heparan sulfate	1093:1107	heparan sulfate	1093:1107	To further understand the cytotoxicity of bleomycin and its relationship to GAGs, we used sodium chlorate to inhibit GAG sulfation and commercially available GAGs to compete for cell surface GAG/bleomycin interactions in seven cell lines including CHO745 defective in both heparan sulfate and chondroitin sulfate biosynthesis.
28982096	11	54	with	effect	1689:1694	arg1	mechanism					1727:1735	largely unknown molecular mechanism	1701:1735	largely unknown molecular mechanism	1701:1735	Thus, GAGs might be the biological relevant molecules that might be related to the bleomycin-induced fibrosis in certain cancer patients, a severe side effect with largely unknown molecular mechanism.
28982096	7	55	theme	cell	1047:1050	arg1	CHO745					1068:1073	CHO745	1068:1073	CHO745 defective in both heparan sulfate and chondroitin sulfate biosynthesis	1068:1144	To further understand the cytotoxicity of bleomycin and its relationship to GAGs, we used sodium chlorate to inhibit GAG sulfation and commercially available GAGs to compete for cell surface GAG/bleomycin interactions in seven cell lines including CHO745 defective in both heparan sulfate and chondroitin sulfate biosynthesis.
28982096	7	55	theme	cell	1047:1050	arg1	lines					1052:1056	seven cell lines	1041:1056	seven cell lines including CHO745 defective in both heparan sulfate and chondroitin sulfate biosynthesis	1041:1144	To further understand the cytotoxicity of bleomycin and its relationship to GAGs, we used sodium chlorate to inhibit GAG sulfation and commercially available GAGs to compete for cell surface GAG/bleomycin interactions in seven cell lines including CHO745 defective in both heparan sulfate and chondroitin sulfate biosynthesis.
28982096	6	56	from	cells	813:817	arg1	analysis					722:729	LC/MS analysis	716:729	LC/MS analysis of GAG disaccharides simultaneously from bleomycin-treated and non-treated cancer cells	716:817	METHODS We used stable isotope labeling coupled with LC/MS analysis of GAG disaccharides simultaneously from bleomycin-treated and non-treated cancer cells.
28982096	6	56	from	cells	813:817	arg1	disaccharides					738:750	GAG disaccharides	734:750	GAG disaccharides simultaneously from bleomycin-treated and non-treated cancer cells	734:817	METHODS We used stable isotope labeling coupled with LC/MS analysis of GAG disaccharides simultaneously from bleomycin-treated and non-treated cancer cells.
28982096	4	57	theme	charged	502:508	arg1	molecules					516:524	positively charged small molecules	491:524	positively charged small molecules	491:524	We also observed that a class of positively charged small molecules has Golgi localization once inside of the cells.
28982096	7	58	theme	defective	1075:1083	arg1	CHO745					1068:1073	CHO745	1068:1073	CHO745 defective in both heparan sulfate and chondroitin sulfate biosynthesis	1068:1144	To further understand the cytotoxicity of bleomycin and its relationship to GAGs, we used sodium chlorate to inhibit GAG sulfation and commercially available GAGs to compete for cell surface GAG/bleomycin interactions in seven cell lines including CHO745 defective in both heparan sulfate and chondroitin sulfate biosynthesis.
28982096	6	59	theme	bleomycin-treated	772:788	arg1	cells					813:817	bleomycin-treated and non-treated cancer cells	772:817	bleomycin-treated and non-treated cancer cells	772:817	METHODS We used stable isotope labeling coupled with LC/MS analysis of GAG disaccharides simultaneously from bleomycin-treated and non-treated cancer cells.
28982096	11	60	theme	unknown	1709:1715	arg1	mechanism					1727:1735	largely unknown molecular mechanism	1701:1735	largely unknown molecular mechanism	1701:1735	Thus, GAGs might be the biological relevant molecules that might be related to the bleomycin-induced fibrosis in certain cancer patients, a severe side effect with largely unknown molecular mechanism.
28982096	11	61	theme	cancer	1658:1663	arg1	patients					1665:1672	certain cancer patients	1650:1672	certain cancer patients	1650:1672	Thus, GAGs might be the biological relevant molecules that might be related to the bleomycin-induced fibrosis in certain cancer patients, a severe side effect with largely unknown molecular mechanism.
28982096	11	62	from	fibrosis	1638:1645	arg1	patients					1665:1672	certain cancer patients	1650:1672	certain cancer patients	1650:1672	Thus, GAGs might be the biological relevant molecules that might be related to the bleomycin-induced fibrosis in certain cancer patients, a severe side effect with largely unknown molecular mechanism.
28982096	4	63	theme	molecules	516:524	arg1	class					482:486	a class	480:486	a class of positively charged small molecules	480:524	We also observed that a class of positively charged small molecules has Golgi localization once inside of the cells.
28982096	7	64	theme	GAG	937:939	arg1	sulfation					941:949	GAG sulfation	937:949	GAG sulfation	937:949	To further understand the cytotoxicity of bleomycin and its relationship to GAGs, we used sodium chlorate to inhibit GAG sulfation and commercially available GAGs to compete for cell surface GAG/bleomycin interactions in seven cell lines including CHO745 defective in both heparan sulfate and chondroitin sulfate biosynthesis.
28982096	6	65	theme	disaccharides	738:750	arg1	analysis					722:729	LC/MS analysis	716:729	LC/MS analysis of GAG disaccharides simultaneously from bleomycin-treated and non-treated cancer cells	716:817	METHODS We used stable isotope labeling coupled with LC/MS analysis of GAG disaccharides simultaneously from bleomycin-treated and non-treated cancer cells.
28982096	1	66	theme	used	144:147	arg1	drug					161:164	a clinically used anti-cancer drug	131:164	a clinically used anti-cancer drug that produces DNA breaks once inside of cells	131:210	BACKGROUND/AIMS Bleomycin is a clinically used anti-cancer drug that produces DNA breaks once inside of cells.
28982096	1	66	theme	used	144:147	arg1	Bleomycin					118:126	BACKGROUND/AIMS Bleomycin	102:126	BACKGROUND/AIMS Bleomycin	102:126	BACKGROUND/AIMS Bleomycin is a clinically used anti-cancer drug that produces DNA breaks once inside of cells.
28982096	7	67	theme	sulfate	1125:1131	arg1	biosynthesis					1133:1144	both heparan sulfate and chondroitin sulfate biosynthesis	1088:1144	both heparan sulfate and chondroitin sulfate biosynthesis	1088:1144	To further understand the cytotoxicity of bleomycin and its relationship to GAGs, we used sodium chlorate to inhibit GAG sulfation and commercially available GAGs to compete for cell surface GAG/bleomycin interactions in seven cell lines including CHO745 defective in both heparan sulfate and chondroitin sulfate biosynthesis.
28982096	8	68	theme	sulfate	1182:1188	arg1	GAG					1190:1192	heparan sulfate GAG	1174:1192	heparan sulfate GAG	1174:1192	RESULTS we discovered that heparan sulfate GAG was significantly undersulfated and the quantity and disaccharide compositions of GAGs were changed in bleomycin-treated cells in a concentration- and time-dependent manner.
28982096	11	69	theme	bleomycin-induced	1620:1636	arg1	fibrosis					1638:1645	the bleomycin-induced fibrosis	1616:1645	the bleomycin-induced fibrosis in certain cancer patients	1616:1672	Thus, GAGs might be the biological relevant molecules that might be related to the bleomycin-induced fibrosis in certain cancer patients, a severe side effect with largely unknown molecular mechanism.
28982096	11	69	theme	bleomycin-induced	1620:1636	arg1	effect					1689:1694	a severe side effect	1675:1694	a severe side effect with largely unknown molecular mechanism	1675:1735	Thus, GAGs might be the biological relevant molecules that might be related to the bleomycin-induced fibrosis in certain cancer patients, a severe side effect with largely unknown molecular mechanism.
28982096	2	70	theme	charged	248:254	arg1	molecule					256:263	a positively charged molecule	235:263	a positively charged molecule	235:263	However, bleomycin is a positively charged molecule and cannot get inside of cells by free diffusion.
28982096	2	70	theme	charged	248:254	arg1	bleomycin					222:230	bleomycin	222:230	bleomycin	222:230	However, bleomycin is a positively charged molecule and cannot get inside of cells by free diffusion.
28982096	6	71	theme	GAG	734:736	arg1	disaccharides					738:750	GAG disaccharides	734:750	GAG disaccharides simultaneously from bleomycin-treated and non-treated cancer cells	734:817	METHODS We used stable isotope labeling coupled with LC/MS analysis of GAG disaccharides simultaneously from bleomycin-treated and non-treated cancer cells.
28982096	1	72	theme	anti-cancer	149:159	arg1	drug					161:164	a clinically used anti-cancer drug	131:164	a clinically used anti-cancer drug that produces DNA breaks once inside of cells	131:210	BACKGROUND/AIMS Bleomycin is a clinically used anti-cancer drug that produces DNA breaks once inside of cells.
28982096	1	72	theme	anti-cancer	149:159	arg1	Bleomycin					118:126	BACKGROUND/AIMS Bleomycin	102:126	BACKGROUND/AIMS Bleomycin	102:126	BACKGROUND/AIMS Bleomycin is a clinically used anti-cancer drug that produces DNA breaks once inside of cells.
28982096	11	73	theme	biological	1561:1570	arg1	molecules					1581:1589	the biological relevant molecules	1557:1589	the biological relevant molecules that might be related to the bleomycin-induced fibrosis in certain cancer patients, a severe side effect with largely unknown molecular mechanism	1557:1735	Thus, GAGs might be the biological relevant molecules that might be related to the bleomycin-induced fibrosis in certain cancer patients, a severe side effect with largely unknown molecular mechanism.
28982096	11	73	theme	biological	1561:1570	arg1	related					1605:1611	related	1605:1611	related	1605:1611	Thus, GAGs might be the biological relevant molecules that might be related to the bleomycin-induced fibrosis in certain cancer patients, a severe side effect with largely unknown molecular mechanism.
28982096	11	73	theme	biological	1561:1570	arg1	GAGs					1543:1546	GAGs	1543:1546	GAGs	1543:1546	Thus, GAGs might be the biological relevant molecules that might be related to the bleomycin-induced fibrosis in certain cancer patients, a severe side effect with largely unknown molecular mechanism.
28982096	7	74	from	defective	1075:1083	arg1	biosynthesis					1133:1144	both heparan sulfate and chondroitin sulfate biosynthesis	1088:1144	both heparan sulfate and chondroitin sulfate biosynthesis	1088:1144	To further understand the cytotoxicity of bleomycin and its relationship to GAGs, we used sodium chlorate to inhibit GAG sulfation and commercially available GAGs to compete for cell surface GAG/bleomycin interactions in seven cell lines including CHO745 defective in both heparan sulfate and chondroitin sulfate biosynthesis.
28982096	8	75	theme	heparan	1174:1180	arg1	GAG					1190:1192	heparan sulfate GAG	1174:1192	heparan sulfate GAG	1174:1192	RESULTS we discovered that heparan sulfate GAG was significantly undersulfated and the quantity and disaccharide compositions of GAGs were changed in bleomycin-treated cells in a concentration- and time-dependent manner.
28982096	11	76	theme	molecular	1717:1725	arg1	mechanism					1727:1735	largely unknown molecular mechanism	1701:1735	largely unknown molecular mechanism	1701:1735	Thus, GAGs might be the biological relevant molecules that might be related to the bleomycin-induced fibrosis in certain cancer patients, a severe side effect with largely unknown molecular mechanism.
28982096	8	77	theme	disaccharide	1247:1258	arg1	compositions					1260:1271	the quantity and disaccharide compositions	1230:1271	compositions	1260:1271	RESULTS we discovered that heparan sulfate GAG was significantly undersulfated and the quantity and disaccharide compositions of GAGs were changed in bleomycin-treated cells in a concentration- and time-dependent manner.
28982096	6	78	dep	METHODS	663:669	arg1	used					674:677	used	674:677	used stable isotope labeling coupled with LC/MS analysis of GAG disaccharides simultaneously from bleomycin-treated and non-treated cancer cells	674:817	METHODS We used stable isotope labeling coupled with LC/MS analysis of GAG disaccharides simultaneously from bleomycin-treated and non-treated cancer cells.
28982096	6	79	from	analysis	722:729	arg1	cells					813:817	bleomycin-treated and non-treated cancer cells	772:817	bleomycin-treated and non-treated cancer cells	772:817	METHODS We used stable isotope labeling coupled with LC/MS analysis of GAG disaccharides simultaneously from bleomycin-treated and non-treated cancer cells.
26797593	9	0	dep	impairs	1232:1238	arg1	Whereas					1205:1211	Whereas	1205:1211	Whereas	1205:1211	Whereas a chitosan coating impairs the accessibility of the microporosity of the zeolite; the presence of the zeolite improves the stability of the dispersion of chitosan upon supercritical drying and increases the affinity of the composites for Cu(II) cations.
26797593	1	1	with	beads	131:135	arg1	contents					178:185	different zeolite contents	160:185	different zeolite contents	160:185	Chitosan-zeolite Na-X composite beads with open porosity and different zeolite contents were prepared by an encapsulation method.
26797593	1	1	with	beads	131:135	arg1	porosity					147:154	open porosity	142:154	open porosity	142:154	Chitosan-zeolite Na-X composite beads with open porosity and different zeolite contents were prepared by an encapsulation method.
26797593	2	2	theme	gelling	340:346	arg1	process					348:354	the polysaccharide gelling process	321:354	the polysaccharide gelling process	321:354	Preparation conditions had to be optimised in order to stabilize the zeolite network during the polysaccharide gelling process.
26797593	5	3	theme	adsorbent	645:653	arg1	storage					630:636	the storage	626:636	the storage of the adsorbent	626:653	The choice of drying method used for the storage of the adsorbent severely affects the textural properties of the composite and the copper sorption effectiveness.
26797593	5	4	theme	drying	603:608	arg1	method					610:615	drying method	603:615	drying method used for the storage of the adsorbent	603:653	The choice of drying method used for the storage of the adsorbent severely affects the textural properties of the composite and the copper sorption effectiveness.
26797593	2	5	theme	polysaccharide	325:338	arg1	process					348:354	the polysaccharide gelling process	321:354	the polysaccharide gelling process	321:354	Preparation conditions had to be optimised in order to stabilize the zeolite network during the polysaccharide gelling process.
26797593	0	6	from	Sorption	0:7	arg1	Composites					46:55	Chitosan-Zeolite X Composites	27:55	Chitosan-Zeolite X Composites	27:55	Sorption of Cu(II) Ions on Chitosan-Zeolite X Composites: Impact of Gelling and Drying Conditions.
26797593	5	7	theme	method	610:615	arg1	choice					593:598	The choice	589:598	The choice of drying method used for the storage of the adsorbent	589:653	The choice of drying method used for the storage of the adsorbent severely affects the textural properties of the composite and the copper sorption effectiveness.
26797593	6	8	theme	sorption	763:770	arg1	mg·g					815:818	about 190 mg·g	805:818	about 190 mg·g	805:818	The copper sorption capacity of chitosan hydrogel is about 190 mg·g(-1).
26797593	6	8	theme	sorption	763:770	arg1	capacity					772:779	The copper sorption capacity	752:779	The copper sorption capacity of chitosan hydrogel	752:800	The copper sorption capacity of chitosan hydrogel is about 190 mg·g(-1).
26797593	5	9	theme	textural	676:683	arg1	properties					685:694	the textural properties	672:694	the textural properties of the composite	672:711	The choice of drying method used for the storage of the adsorbent severely affects the textural properties of the composite and the copper sorption effectiveness.
26797593	9	10	theme	supercritical	1381:1393	arg1	drying					1395:1400	supercritical drying	1381:1400	supercritical drying	1381:1400	Whereas a chitosan coating impairs the accessibility of the microporosity of the zeolite; the presence of the zeolite improves the stability of the dispersion of chitosan upon supercritical drying and increases the affinity of the composites for Cu(II) cations.
26797593	6	11	theme	copper	756:761	arg1	mg·g					815:818	about 190 mg·g	805:818	about 190 mg·g	805:818	The copper sorption capacity of chitosan hydrogel is about 190 mg·g(-1).
26797593	6	11	theme	copper	756:761	arg1	capacity					772:779	The copper sorption capacity	752:779	The copper sorption capacity of chitosan hydrogel	752:800	The copper sorption capacity of chitosan hydrogel is about 190 mg·g(-1).
26797593	0	12	dep	Gelling	68:74	arg1	Conditions					87:96	Conditions	87:96	Conditions	87:96	Sorption of Cu(II) Ions on Chitosan-Zeolite X Composites: Impact of Gelling and Drying Conditions.
26797593	1	13	theme	Chitosan-zeolite	99:114	arg1	beads					131:135	Chitosan-zeolite Na-X composite beads	99:135	Chitosan-zeolite Na-X composite beads with open porosity and different zeolite contents	99:185	Chitosan-zeolite Na-X composite beads with open porosity and different zeolite contents were prepared by an encapsulation method.
26797593	10	14	theme	Chitosan-zeolite	1467:1482	arg1	aerogels					1484:1491	Chitosan-zeolite aerogels	1467:1491	Chitosan-zeolite aerogels	1467:1491	Chitosan-zeolite aerogels present Cu(II) sorption properties.
26797593	10	15	theme	sorption	1508:1515	arg1	properties					1517:1526	Cu(II) sorption properties	1501:1526	Cu(II) sorption properties	1501:1526	Chitosan-zeolite aerogels present Cu(II) sorption properties.
26797593	1	16	theme	open	142:145	arg1	porosity					147:154	open porosity	142:154	open porosity	142:154	Chitosan-zeolite Na-X composite beads with open porosity and different zeolite contents were prepared by an encapsulation method.
26797593	0	17	theme	Gelling	68:74	arg1	Impact					58:63	Impact	58:63	Sorption of Cu(II) Ions on Chitosan-Zeolite X Composites: Impact of Gelling and Drying Conditions.	0:97	Sorption of Cu(II) Ions on Chitosan-Zeolite X Composites: Impact of Gelling and Drying Conditions.
26797593	7	18	theme	evaporative	994:1004	arg1	drying					1006:1011	evaporative drying	994:1011	evaporative drying to a xerogel	994:1024	More than 70% of this capacity is retained when the polysaccharide is stored as an aerogel after supercrititcal CO₂ drying, but nearly 90% of the capacity is lost after evaporative drying to a xerogel.
26797593	7	19	theme	capacity	971:978	arg1	%					962:962	nearly 90%	953:962	nearly 90% of the capacity	953:978	More than 70% of this capacity is retained when the polysaccharide is stored as an aerogel after supercrititcal CO₂ drying, but nearly 90% of the capacity is lost after evaporative drying to a xerogel.
26797593	7	19	theme	capacity	971:978	arg1	capacity					971:978	the capacity	967:978	the capacity	967:978	More than 70% of this capacity is retained when the polysaccharide is stored as an aerogel after supercrititcal CO₂ drying, but nearly 90% of the capacity is lost after evaporative drying to a xerogel.
26797593	3	20	theme	thermogravimetric	514:530	arg1	analysis					532:539	thermogravimetric analysis	514:539	thermogravimetric analysis (TG)	514:544	Composites and pure reference components were characterized using X-ray diffraction (XRD); scanning electron microscopy (SEM); N₂ adsorption-desorption; and thermogravimetric analysis (TG).
26797593	3	20	theme	thermogravimetric	514:530	arg1	TG					542:543	TG	542:543	TG	542:543	Composites and pure reference components were characterized using X-ray diffraction (XRD); scanning electron microscopy (SEM); N₂ adsorption-desorption; and thermogravimetric analysis (TG).
26797593	8	21	theme	zeolite-polysaccharide	1102:1123	arg1	composites					1125:1134	the zeolite-polysaccharide composites	1098:1134	the zeolite-polysaccharide composites	1098:1134	Textural data and Cu(II) sorption data indicate that the properties of the zeolite-polysaccharide composites are not just the sum of the properties of the individual components.
26797593	3	22	theme	pure	372:375	arg1	components					387:396	pure reference components	372:396	pure reference components	372:396	Composites and pure reference components were characterized using X-ray diffraction (XRD); scanning electron microscopy (SEM); N₂ adsorption-desorption; and thermogravimetric analysis (TG).
26797593	8	23	theme	Textural	1027:1034	arg1	data					1036:1039	Textural data	1027:1039	Textural data	1027:1039	Textural data and Cu(II) sorption data indicate that the properties of the zeolite-polysaccharide composites are not just the sum of the properties of the individual components.
26797593	3	24	theme	X-ray	423:427	arg1	XRD					442:444	XRD	442:444	XRD	442:444	Composites and pure reference components were characterized using X-ray diffraction (XRD); scanning electron microscopy (SEM); N₂ adsorption-desorption; and thermogravimetric analysis (TG).
26797593	3	24	theme	X-ray	423:427	arg1	diffraction					429:439	X-ray diffraction	423:439	X-ray diffraction (XRD)	423:445	Composites and pure reference components were characterized using X-ray diffraction (XRD); scanning electron microscopy (SEM); N₂ adsorption-desorption; and thermogravimetric analysis (TG).
26797593	3	25	theme	reference	377:385	arg1	components					387:396	pure reference components	372:396	pure reference components	372:396	Composites and pure reference components were characterized using X-ray diffraction (XRD); scanning electron microscopy (SEM); N₂ adsorption-desorption; and thermogravimetric analysis (TG).
26797593	8	26	theme	composites	1125:1134	arg1	properties					1084:1093	the properties	1080:1093	the properties of the zeolite-polysaccharide composites	1080:1134	Textural data and Cu(II) sorption data indicate that the properties of the zeolite-polysaccharide composites are not just the sum of the properties of the individual components.
26797593	8	27	dep	are	1136:1138	arg1	just					1144:1147	just	1144:1147	just	1144:1147	Textural data and Cu(II) sorption data indicate that the properties of the zeolite-polysaccharide composites are not just the sum of the properties of the individual components.
26797593	1	28	theme	different	160:168	arg1	contents					178:185	different zeolite contents	160:185	different zeolite contents	160:185	Chitosan-zeolite Na-X composite beads with open porosity and different zeolite contents were prepared by an encapsulation method.
26797593	9	29	theme	chitosan	1215:1222	arg1	coating					1224:1230	a chitosan coating	1213:1230	a chitosan coating	1213:1230	Whereas a chitosan coating impairs the accessibility of the microporosity of the zeolite; the presence of the zeolite improves the stability of the dispersion of chitosan upon supercritical drying and increases the affinity of the composites for Cu(II) cations.
26797593	7	30	theme	capacity	847:854	arg1	%					837:837	More than 70%	825:837	More than 70% of this capacity	825:854	More than 70% of this capacity is retained when the polysaccharide is stored as an aerogel after supercrititcal CO₂ drying, but nearly 90% of the capacity is lost after evaporative drying to a xerogel.
26797593	7	30	theme	capacity	847:854	arg1	capacity					847:854	this capacity	842:854	this capacity	842:854	More than 70% of this capacity is retained when the polysaccharide is stored as an aerogel after supercrititcal CO₂ drying, but nearly 90% of the capacity is lost after evaporative drying to a xerogel.
26797593	1	31	theme	zeolite	170:176	arg1	contents					178:185	different zeolite contents	160:185	different zeolite contents	160:185	Chitosan-zeolite Na-X composite beads with open porosity and different zeolite contents were prepared by an encapsulation method.
26797593	0	32	theme	Cu	12:13	arg1	Ions					19:22	Cu(II) Ions	12:22	Cu(II) Ions	12:22	Sorption of Cu(II) Ions on Chitosan-Zeolite X Composites: Impact of Gelling and Drying Conditions.
26797593	4	33	theme	Cu	547:548	arg1	sorption					554:561	Cu(II) sorption	547:561	Cu(II) sorption	547:561	Cu(II) sorption was investigated at pH 6.
26797593	0	34	theme	Drying	80:85	arg1	Impact					58:63	Impact	58:63	Sorption of Cu(II) Ions on Chitosan-Zeolite X Composites: Impact of Gelling and Drying Conditions.	0:97	Sorption of Cu(II) Ions on Chitosan-Zeolite X Composites: Impact of Gelling and Drying Conditions.
26797593	3	35	theme	N₂	484:485	arg1	adsorption-desorption					487:507	N₂ adsorption-desorption	484:507	N₂ adsorption-desorption	484:507	Composites and pure reference components were characterized using X-ray diffraction (XRD); scanning electron microscopy (SEM); N₂ adsorption-desorption; and thermogravimetric analysis (TG).
26797593	3	36	theme	electron	457:464	arg1	SEM					478:480	SEM	478:480	SEM	478:480	Composites and pure reference components were characterized using X-ray diffraction (XRD); scanning electron microscopy (SEM); N₂ adsorption-desorption; and thermogravimetric analysis (TG).
26797593	3	36	theme	electron	457:464	arg1	microscopy					466:475	scanning electron microscopy	448:475	scanning electron microscopy (SEM)	448:481	Composites and pure reference components were characterized using X-ray diffraction (XRD); scanning electron microscopy (SEM); N₂ adsorption-desorption; and thermogravimetric analysis (TG).
26797593	5	37	theme	copper	721:726	arg1	effectiveness					737:749	the copper sorption effectiveness	717:749	the copper sorption effectiveness	717:749	The choice of drying method used for the storage of the adsorbent severely affects the textural properties of the composite and the copper sorption effectiveness.
26797593	9	38	theme	chitosan	1367:1374	arg1	dispersion					1353:1362	the dispersion	1349:1362	the dispersion of chitosan	1349:1374	Whereas a chitosan coating impairs the accessibility of the microporosity of the zeolite; the presence of the zeolite improves the stability of the dispersion of chitosan upon supercritical drying and increases the affinity of the composites for Cu(II) cations.
26797593	8	39	theme	components	1193:1202	arg1	properties					1164:1173	the properties	1160:1173	the properties of the individual components	1160:1202	Textural data and Cu(II) sorption data indicate that the properties of the zeolite-polysaccharide composites are not just the sum of the properties of the individual components.
26797593	5	40	theme	sorption	728:735	arg1	effectiveness					737:749	the copper sorption effectiveness	717:749	the copper sorption effectiveness	717:749	The choice of drying method used for the storage of the adsorbent severely affects the textural properties of the composite and the copper sorption effectiveness.
26797593	10	41	theme	Cu	1501:1502	arg1	properties					1517:1526	Cu(II) sorption properties	1501:1526	Cu(II) sorption properties	1501:1526	Chitosan-zeolite aerogels present Cu(II) sorption properties.
26797593	8	42	theme	individual	1182:1191	arg1	components					1193:1202	the individual components	1178:1202	the individual components	1178:1202	Textural data and Cu(II) sorption data indicate that the properties of the zeolite-polysaccharide composites are not just the sum of the properties of the individual components.
26797593	7	43	theme	CO₂	937:939	arg1	drying					941:946	supercrititcal CO₂ drying	922:946	supercrititcal CO₂ drying	922:946	More than 70% of this capacity is retained when the polysaccharide is stored as an aerogel after supercrititcal CO₂ drying, but nearly 90% of the capacity is lost after evaporative drying to a xerogel.
26797593	9	44	theme	dispersion	1353:1362	arg1	stability					1336:1344	the stability	1332:1344	the stability of the dispersion of chitosan	1332:1374	Whereas a chitosan coating impairs the accessibility of the microporosity of the zeolite; the presence of the zeolite improves the stability of the dispersion of chitosan upon supercritical drying and increases the affinity of the composites for Cu(II) cations.
26797593	5	45	theme	composite	703:711	arg1	properties					685:694	the textural properties	672:694	the textural properties of the composite	672:711	The choice of drying method used for the storage of the adsorbent severely affects the textural properties of the composite and the copper sorption effectiveness.
26797593	5	45	theme	composite	703:711	arg1	effectiveness					737:749	the copper sorption effectiveness	717:749	the copper sorption effectiveness	717:749	The choice of drying method used for the storage of the adsorbent severely affects the textural properties of the composite and the copper sorption effectiveness.
26797593	8	46	theme	properties	1164:1173	arg1	sum					1153:1155	the sum	1149:1155	the sum of the properties of the individual components	1149:1202	Textural data and Cu(II) sorption data indicate that the properties of the zeolite-polysaccharide composites are not just the sum of the properties of the individual components.
26797593	0	47	theme	Ions	19:22	arg1	Sorption					0:7	Sorption	0:7	Sorption of Cu(II) Ions on Chitosan-Zeolite X Composites: Impact of Gelling and Drying Conditions.	0:97	Sorption of Cu(II) Ions on Chitosan-Zeolite X Composites: Impact of Gelling and Drying Conditions.
26797593	9	48	theme	Cu	1451:1452	arg1	cations					1458:1464	Cu(II) cations	1451:1464	Cu(II) cations	1451:1464	Whereas a chitosan coating impairs the accessibility of the microporosity of the zeolite; the presence of the zeolite improves the stability of the dispersion of chitosan upon supercritical drying and increases the affinity of the composites for Cu(II) cations.
26797593	6	49	dep	mg·g	815:818	arg1	-1					820:821	-1	820:821	-1	820:821	The copper sorption capacity of chitosan hydrogel is about 190 mg·g(-1).
26797593	6	50	theme	hydrogel	793:800	arg1	mg·g					815:818	about 190 mg·g	805:818	about 190 mg·g	805:818	The copper sorption capacity of chitosan hydrogel is about 190 mg·g(-1).
26797593	6	50	theme	hydrogel	793:800	arg1	capacity					772:779	The copper sorption capacity	752:779	The copper sorption capacity of chitosan hydrogel	752:800	The copper sorption capacity of chitosan hydrogel is about 190 mg·g(-1).
26797593	7	51	theme	supercrititcal	922:935	arg1	drying					941:946	supercrititcal CO₂ drying	922:946	supercrititcal CO₂ drying	922:946	More than 70% of this capacity is retained when the polysaccharide is stored as an aerogel after supercrititcal CO₂ drying, but nearly 90% of the capacity is lost after evaporative drying to a xerogel.
26797593	9	52	theme	microporosity	1265:1277	arg1	accessibility					1244:1256	the accessibility	1240:1256	the accessibility of the microporosity of the zeolite	1240:1292	Whereas a chitosan coating impairs the accessibility of the microporosity of the zeolite; the presence of the zeolite improves the stability of the dispersion of chitosan upon supercritical drying and increases the affinity of the composites for Cu(II) cations.
26797593	9	53	theme	composites	1436:1445	arg1	affinity					1420:1427	the affinity	1416:1427	the affinity of the composites for Cu(II) cations	1416:1464	Whereas a chitosan coating impairs the accessibility of the microporosity of the zeolite; the presence of the zeolite improves the stability of the dispersion of chitosan upon supercritical drying and increases the affinity of the composites for Cu(II) cations.
26797593	6	54	theme	chitosan	784:791	arg1	hydrogel					793:800	chitosan hydrogel	784:800	chitosan hydrogel	784:800	The copper sorption capacity of chitosan hydrogel is about 190 mg·g(-1).
26797593	8	55	theme	sorption	1052:1059	arg1	data					1061:1064	Cu(II) sorption data	1045:1064	Cu(II) sorption data	1045:1064	Textural data and Cu(II) sorption data indicate that the properties of the zeolite-polysaccharide composites are not just the sum of the properties of the individual components.
26797593	2	56	theme	zeolite	298:304	arg1	network					306:312	the zeolite network	294:312	the zeolite network	294:312	Preparation conditions had to be optimised in order to stabilize the zeolite network during the polysaccharide gelling process.
26797593	0	57	theme	X	44:44	arg1	Composites					46:55	Chitosan-Zeolite X Composites	27:55	Chitosan-Zeolite X Composites	27:55	Sorption of Cu(II) Ions on Chitosan-Zeolite X Composites: Impact of Gelling and Drying Conditions.
26797593	2	58	theme	Preparation	229:239	arg1	conditions					241:250	Preparation conditions	229:250	Preparation conditions	229:250	Preparation conditions had to be optimised in order to stabilize the zeolite network during the polysaccharide gelling process.
26797593	3	59	theme	scanning	448:455	arg1	SEM					478:480	SEM	478:480	SEM	478:480	Composites and pure reference components were characterized using X-ray diffraction (XRD); scanning electron microscopy (SEM); N₂ adsorption-desorption; and thermogravimetric analysis (TG).
26797593	3	59	theme	scanning	448:455	arg1	microscopy					466:475	scanning electron microscopy	448:475	scanning electron microscopy (SEM)	448:481	Composites and pure reference components were characterized using X-ray diffraction (XRD); scanning electron microscopy (SEM); N₂ adsorption-desorption; and thermogravimetric analysis (TG).
26797593	1	60	theme	Na-X	116:119	arg1	beads					131:135	Chitosan-zeolite Na-X composite beads	99:135	Chitosan-zeolite Na-X composite beads with open porosity and different zeolite contents	99:185	Chitosan-zeolite Na-X composite beads with open porosity and different zeolite contents were prepared by an encapsulation method.
26797593	9	61	theme	zeolite	1315:1321	arg1	presence					1299:1306	the presence	1295:1306	the presence of the zeolite	1295:1321	Whereas a chitosan coating impairs the accessibility of the microporosity of the zeolite; the presence of the zeolite improves the stability of the dispersion of chitosan upon supercritical drying and increases the affinity of the composites for Cu(II) cations.
26797593	1	62	theme	encapsulation	207:219	arg1	method					221:226	an encapsulation method	204:226	an encapsulation method	204:226	Chitosan-zeolite Na-X composite beads with open porosity and different zeolite contents were prepared by an encapsulation method.
26797593	0	63	theme	Chitosan-Zeolite	27:42	arg1	Composites					46:55	Chitosan-Zeolite X Composites	27:55	Chitosan-Zeolite X Composites	27:55	Sorption of Cu(II) Ions on Chitosan-Zeolite X Composites: Impact of Gelling and Drying Conditions.
26797593	0	64	dep	Sorption	0:7	arg1	Impact					58:63	Impact	58:63	Sorption of Cu(II) Ions on Chitosan-Zeolite X Composites: Impact of Gelling and Drying Conditions.	0:97	Sorption of Cu(II) Ions on Chitosan-Zeolite X Composites: Impact of Gelling and Drying Conditions.
26797593	1	65	theme	composite	121:129	arg1	beads					131:135	Chitosan-zeolite Na-X composite beads	99:135	Chitosan-zeolite Na-X composite beads with open porosity and different zeolite contents	99:185	Chitosan-zeolite Na-X composite beads with open porosity and different zeolite contents were prepared by an encapsulation method.
26797593	9	66	theme	zeolite	1286:1292	arg1	microporosity					1265:1277	the microporosity	1261:1277	the microporosity of the zeolite	1261:1292	Whereas a chitosan coating impairs the accessibility of the microporosity of the zeolite; the presence of the zeolite improves the stability of the dispersion of chitosan upon supercritical drying and increases the affinity of the composites for Cu(II) cations.
26797593	8	67	theme	Cu	1045:1046	arg1	data					1061:1064	Cu(II) sorption data	1045:1064	Cu(II) sorption data	1045:1064	Textural data and Cu(II) sorption data indicate that the properties of the zeolite-polysaccharide composites are not just the sum of the properties of the individual components.
28492492	2	0	theme	supplement	592:601	arg1	effects					566:572	the effects	562:572	the effects of a whey protein supplement (PRO, n = 38) and a maltodextrin supplement (CHO, n = 46) on bone density	562:675	We conducted an ancillary study to a randomized, double-blind trial comparing the effects of a whey protein supplement (PRO, n = 38) and a maltodextrin supplement (CHO, n = 46) on bone density to evaluate the impact of a calibrated increase in GI/GL on inflammation, insulin resistance, and body composition in a healthy aging population.
28492492	2	1	theme	insulin	751:757	arg1	resistance					759:768	insulin resistance	751:768	insulin resistance	751:768	We conducted an ancillary study to a randomized, double-blind trial comparing the effects of a whey protein supplement (PRO, n = 38) and a maltodextrin supplement (CHO, n = 46) on bone density to evaluate the impact of a calibrated increase in GI/GL on inflammation, insulin resistance, and body composition in a healthy aging population.
28492492	2	2	theme	aging	805:809	arg1	population					811:820	a healthy aging population	795:820	a healthy aging population	795:820	We conducted an ancillary study to a randomized, double-blind trial comparing the effects of a whey protein supplement (PRO, n = 38) and a maltodextrin supplement (CHO, n = 46) on bone density to evaluate the impact of a calibrated increase in GI/GL on inflammation, insulin resistance, and body composition in a healthy aging population.
28492492	1	3	theme	age-related	416:426	arg1	alterations					428:438	age-related alterations	416:438	age-related alterations in body composition and insulin resistance	416:481	Controversy exists as to whether high glycemic index/glycemic load (GI/GL) diets increase the risk of chronic inflammation, which has been postulated as a pathogenic intermediary between such diets and age-related alterations in body composition and insulin resistance.
28492492	1	4	theme	high	247:250	arg1	diets					289:293	high glycemic index/glycemic load (GI/GL) diets	247:293	high glycemic index/glycemic load (GI/GL) diets	247:293	Controversy exists as to whether high glycemic index/glycemic load (GI/GL) diets increase the risk of chronic inflammation, which has been postulated as a pathogenic intermediary between such diets and age-related alterations in body composition and insulin resistance.
28492492	2	5	theme	whey	579:582	arg1	PRO					604:606	PRO	604:606	PRO	604:606	We conducted an ancillary study to a randomized, double-blind trial comparing the effects of a whey protein supplement (PRO, n = 38) and a maltodextrin supplement (CHO, n = 46) on bone density to evaluate the impact of a calibrated increase in GI/GL on inflammation, insulin resistance, and body composition in a healthy aging population.
28492492	2	5	theme	whey	579:582	arg1	supplement					592:601	a whey protein supplement	577:601	a whey protein supplement (PRO, n = 38)	577:615	We conducted an ancillary study to a randomized, double-blind trial comparing the effects of a whey protein supplement (PRO, n = 38) and a maltodextrin supplement (CHO, n = 46) on bone density to evaluate the impact of a calibrated increase in GI/GL on inflammation, insulin resistance, and body composition in a healthy aging population.
28492492	1	6	theme	index/glycemic	261:274	arg1	GI/GL					282:286	GI/GL	282:286	GI/GL	282:286	Controversy exists as to whether high glycemic index/glycemic load (GI/GL) diets increase the risk of chronic inflammation, which has been postulated as a pathogenic intermediary between such diets and age-related alterations in body composition and insulin resistance.
28492492	1	6	theme	index/glycemic	261:274	arg1	load					276:279	glycemic index/glycemic load	252:279	high glycemic index/glycemic load (GI/GL) diets	247:293	Controversy exists as to whether high glycemic index/glycemic load (GI/GL) diets increase the risk of chronic inflammation, which has been postulated as a pathogenic intermediary between such diets and age-related alterations in body composition and insulin resistance.
28492492	6	7	from	GL	1486:1487	arg1	ratio					1610:1614	the lean-to-fat mass ratio	1589:1614	the lean-to-fat mass ratio (r = -0.3, p = 0.002)	1589:1636	However, trunk lean mass (p = 0.0375) and total lean mass (p = 0.038) were higher in the PRO group compared to the CHO group at 18 months There were also significant associations for GL and change in total fat mass (r = 0.3, p = 0.01), change in BMI (r = 0.3, p = 0.005), and change in the lean-to-fat mass ratio (r = -0.3, p = 0.002).
28492492	6	7	from	GL	1486:1487	arg1	BMI					1549:1551	BMI	1549:1551	BMI (r = 0.3, p = 0.005)	1549:1572	However, trunk lean mass (p = 0.0375) and total lean mass (p = 0.038) were higher in the PRO group compared to the CHO group at 18 months There were also significant associations for GL and change in total fat mass (r = 0.3, p = 0.01), change in BMI (r = 0.3, p = 0.005), and change in the lean-to-fat mass ratio (r = -0.3, p = 0.002).
28492492	6	7	from	GL	1486:1487	arg1	mass					1513:1516	total fat mass	1503:1516	total fat mass (r = 0.3, p = 0.01)	1503:1536	However, trunk lean mass (p = 0.0375) and total lean mass (p = 0.038) were higher in the PRO group compared to the CHO group at 18 months There were also significant associations for GL and change in total fat mass (r = 0.3, p = 0.01), change in BMI (r = 0.3, p = 0.005), and change in the lean-to-fat mass ratio (r = -0.3, p = 0.002).
28492492	3	8	theme	food	904:907	arg1	records					909:915	3-day food records	898:915	3-day food records	898:915	Markers of inflammation, HOMA, body composition, and GI/GL (estimated from 3-day food records) were assessed at baseline and 18 months.
28492492	4	9	theme	CHO	987:989	arg1	group					991:995	the CHO group	983:995	the CHO group	983:995	By 18 months, the GL in the CHO group increased by 34%, 88.4 ± 5.2 → 118.5 ± 4.9 and did not change in the PRO group, 86.5 ± 4.1 → 82.0 ± 3.6 (p < 0.0001).
28492492	0	10	theme	Protein	183:189	arg1	Trial					207:211	a Multicenter Protein Supplementation Trial	169:211	a Multicenter Protein Supplementation Trial	169:211	The Effect of Dietary Glycemic Properties on Markers of Inflammation, Insulin Resistance, and Body Composition in Postmenopausal American Women: An Ancillary Study from a Multicenter Protein Supplementation Trial.
28492492	5	11	theme	serum	1164:1168	arg1	CRP					1170:1172	serum CRP	1164:1172	serum CRP	1164:1172	Despite this change there were no differences in serum CRP, IL-6, or HOMA at 18 months between the two groups, nor were there significant associations between GL and inflammatory markers.
28492492	2	12	dep	PRO	604:606	arg1	n					609:609	n = 38	609:614	n = 38	609:614	We conducted an ancillary study to a randomized, double-blind trial comparing the effects of a whey protein supplement (PRO, n = 38) and a maltodextrin supplement (CHO, n = 46) on bone density to evaluate the impact of a calibrated increase in GI/GL on inflammation, insulin resistance, and body composition in a healthy aging population.
28492492	6	13	from	change	1493:1498	arg1	ratio					1610:1614	the lean-to-fat mass ratio	1589:1614	the lean-to-fat mass ratio (r = -0.3, p = 0.002)	1589:1636	However, trunk lean mass (p = 0.0375) and total lean mass (p = 0.038) were higher in the PRO group compared to the CHO group at 18 months There were also significant associations for GL and change in total fat mass (r = 0.3, p = 0.01), change in BMI (r = 0.3, p = 0.005), and change in the lean-to-fat mass ratio (r = -0.3, p = 0.002).
28492492	6	13	from	change	1493:1498	arg1	BMI					1549:1551	BMI	1549:1551	BMI (r = 0.3, p = 0.005)	1549:1572	However, trunk lean mass (p = 0.0375) and total lean mass (p = 0.038) were higher in the PRO group compared to the CHO group at 18 months There were also significant associations for GL and change in total fat mass (r = 0.3, p = 0.01), change in BMI (r = 0.3, p = 0.005), and change in the lean-to-fat mass ratio (r = -0.3, p = 0.002).
28492492	6	13	from	change	1493:1498	arg1	mass					1513:1516	total fat mass	1503:1516	total fat mass (r = 0.3, p = 0.01)	1503:1536	However, trunk lean mass (p = 0.0375) and total lean mass (p = 0.038) were higher in the PRO group compared to the CHO group at 18 months There were also significant associations for GL and change in total fat mass (r = 0.3, p = 0.01), change in BMI (r = 0.3, p = 0.005), and change in the lean-to-fat mass ratio (r = -0.3, p = 0.002).
28492492	0	14	theme	Inflammation	56:67	arg1	Markers					45:51	Markers	45:51	Markers of Inflammation, Insulin Resistance, and Body Composition in Postmenopausal American Women	45:142	The Effect of Dietary Glycemic Properties on Markers of Inflammation, Insulin Resistance, and Body Composition in Postmenopausal American Women: An Ancillary Study from a Multicenter Protein Supplementation Trial.
28492492	1	15	theme	insulin	464:470	arg1	resistance					472:481	insulin resistance	464:481	insulin resistance	464:481	Controversy exists as to whether high glycemic index/glycemic load (GI/GL) diets increase the risk of chronic inflammation, which has been postulated as a pathogenic intermediary between such diets and age-related alterations in body composition and insulin resistance.
28492492	3	16	dep	GI/GL	876:880	arg1	estimated					883:891	estimated	883:891	estimated from 3-day food records	883:915	Markers of inflammation, HOMA, body composition, and GI/GL (estimated from 3-day food records) were assessed at baseline and 18 months.
28492492	0	17	theme	Composition	99:109	arg1	Markers					45:51	Markers	45:51	Markers of Inflammation, Insulin Resistance, and Body Composition in Postmenopausal American Women	45:142	The Effect of Dietary Glycemic Properties on Markers of Inflammation, Insulin Resistance, and Body Composition in Postmenopausal American Women: An Ancillary Study from a Multicenter Protein Supplementation Trial.
28492492	5	18	from	months	1195:1200	arg1	HOMA					1184:1187	HOMA	1184:1187	HOMA	1184:1187	Despite this change there were no differences in serum CRP, IL-6, or HOMA at 18 months between the two groups, nor were there significant associations between GL and inflammatory markers.
28492492	5	18	from	months	1195:1200	arg1	IL-6					1175:1178	IL-6	1175:1178	IL-6	1175:1178	Despite this change there were no differences in serum CRP, IL-6, or HOMA at 18 months between the two groups, nor were there significant associations between GL and inflammatory markers.
28492492	5	18	from	months	1195:1200	arg1	differences					1149:1159	no differences	1146:1159	no differences in serum CRP, IL-6, or HOMA at 18 months between the two groups	1146:1223	Despite this change there were no differences in serum CRP, IL-6, or HOMA at 18 months between the two groups, nor were there significant associations between GL and inflammatory markers.
28492492	5	18	from	months	1195:1200	arg1	CRP					1170:1172	serum CRP	1164:1172	serum CRP	1164:1172	Despite this change there were no differences in serum CRP, IL-6, or HOMA at 18 months between the two groups, nor were there significant associations between GL and inflammatory markers.
28492492	2	19	from	composition	780:790	arg1	population					811:820	a healthy aging population	795:820	a healthy aging population	795:820	We conducted an ancillary study to a randomized, double-blind trial comparing the effects of a whey protein supplement (PRO, n = 38) and a maltodextrin supplement (CHO, n = 46) on bone density to evaluate the impact of a calibrated increase in GI/GL on inflammation, insulin resistance, and body composition in a healthy aging population.
28492492	0	20	theme	Postmenopausal	114:127	arg1	Women					138:142	Postmenopausal American Women	114:142	Postmenopausal American Women	114:142	The Effect of Dietary Glycemic Properties on Markers of Inflammation, Insulin Resistance, and Body Composition in Postmenopausal American Women: An Ancillary Study from a Multicenter Protein Supplementation Trial.
28492492	6	21	theme	=	1556:1556	arg1	r					1554:1554	r	1554:1554	r = 0.3	1554:1560	However, trunk lean mass (p = 0.0375) and total lean mass (p = 0.038) were higher in the PRO group compared to the CHO group at 18 months There were also significant associations for GL and change in total fat mass (r = 0.3, p = 0.01), change in BMI (r = 0.3, p = 0.005), and change in the lean-to-fat mass ratio (r = -0.3, p = 0.002).
28492492	6	22	theme	lean	1318:1321	arg1	p					1329:1329	p = 0.0375	1329:1338	p = 0.0375	1329:1338	However, trunk lean mass (p = 0.0375) and total lean mass (p = 0.038) were higher in the PRO group compared to the CHO group at 18 months There were also significant associations for GL and change in total fat mass (r = 0.3, p = 0.01), change in BMI (r = 0.3, p = 0.005), and change in the lean-to-fat mass ratio (r = -0.3, p = 0.002).
28492492	6	22	theme	lean	1318:1321	arg1	mass					1323:1326	trunk lean mass	1312:1326	trunk lean mass (p = 0.0375)	1312:1339	However, trunk lean mass (p = 0.0375) and total lean mass (p = 0.038) were higher in the PRO group compared to the CHO group at 18 months There were also significant associations for GL and change in total fat mass (r = 0.3, p = 0.01), change in BMI (r = 0.3, p = 0.005), and change in the lean-to-fat mass ratio (r = -0.3, p = 0.002).
28492492	6	23	theme	=	1364:1364	arg1	0.038					1366:1370	p = 0.038	1362:1370	p = 0.038	1362:1370	However, trunk lean mass (p = 0.0375) and total lean mass (p = 0.038) were higher in the PRO group compared to the CHO group at 18 months There were also significant associations for GL and change in total fat mass (r = 0.3, p = 0.01), change in BMI (r = 0.3, p = 0.005), and change in the lean-to-fat mass ratio (r = -0.3, p = 0.002).
28492492	6	23	theme	=	1364:1364	arg1	mass					1356:1359	total lean mass	1345:1359	total lean mass (p = 0.038)	1345:1371	However, trunk lean mass (p = 0.0375) and total lean mass (p = 0.038) were higher in the PRO group compared to the CHO group at 18 months There were also significant associations for GL and change in total fat mass (r = 0.3, p = 0.01), change in BMI (r = 0.3, p = 0.005), and change in the lean-to-fat mass ratio (r = -0.3, p = 0.002).
28492492	7	24	theme	detectable	1724:1733	arg1	change					1735:1740	no detectable change	1721:1740	no detectable change	1721:1740	Our data suggest that as dietary GL increases within the moderate range, there is no detectable change in markers of inflammation or insulin resistance, despite which there is a negative effect on body composition.
28492492	0	25	from	Resistance	78:87	arg1	Women					138:142	Postmenopausal American Women	114:142	Postmenopausal American Women	114:142	The Effect of Dietary Glycemic Properties on Markers of Inflammation, Insulin Resistance, and Body Composition in Postmenopausal American Women: An Ancillary Study from a Multicenter Protein Supplementation Trial.
28492492	6	26	dep	=	1556:1556	arg1	0.3					1558:1560	0.3	1558:1560	0.3	1558:1560	However, trunk lean mass (p = 0.0375) and total lean mass (p = 0.038) were higher in the PRO group compared to the CHO group at 18 months There were also significant associations for GL and change in total fat mass (r = 0.3, p = 0.01), change in BMI (r = 0.3, p = 0.005), and change in the lean-to-fat mass ratio (r = -0.3, p = 0.002).
28492492	6	27	dep	=	1619:1619	arg1	-0.3					1621:1624	-0.3	1621:1624	-0.3	1621:1624	However, trunk lean mass (p = 0.0375) and total lean mass (p = 0.038) were higher in the PRO group compared to the CHO group at 18 months There were also significant associations for GL and change in total fat mass (r = 0.3, p = 0.01), change in BMI (r = 0.3, p = 0.005), and change in the lean-to-fat mass ratio (r = -0.3, p = 0.002).
28492492	6	28	theme	CHO	1418:1420	arg1	group					1422:1426	the CHO group	1414:1426	the CHO group	1414:1426	However, trunk lean mass (p = 0.0375) and total lean mass (p = 0.038) were higher in the PRO group compared to the CHO group at 18 months There were also significant associations for GL and change in total fat mass (r = 0.3, p = 0.01), change in BMI (r = 0.3, p = 0.005), and change in the lean-to-fat mass ratio (r = -0.3, p = 0.002).
28492492	6	29	from	change	1539:1544	arg1	ratio					1610:1614	the lean-to-fat mass ratio	1589:1614	the lean-to-fat mass ratio (r = -0.3, p = 0.002)	1589:1636	However, trunk lean mass (p = 0.0375) and total lean mass (p = 0.038) were higher in the PRO group compared to the CHO group at 18 months There were also significant associations for GL and change in total fat mass (r = 0.3, p = 0.01), change in BMI (r = 0.3, p = 0.005), and change in the lean-to-fat mass ratio (r = -0.3, p = 0.002).
28492492	6	29	from	change	1539:1544	arg1	BMI					1549:1551	BMI	1549:1551	BMI (r = 0.3, p = 0.005)	1549:1572	However, trunk lean mass (p = 0.0375) and total lean mass (p = 0.038) were higher in the PRO group compared to the CHO group at 18 months There were also significant associations for GL and change in total fat mass (r = 0.3, p = 0.01), change in BMI (r = 0.3, p = 0.005), and change in the lean-to-fat mass ratio (r = -0.3, p = 0.002).
28492492	6	29	from	change	1539:1544	arg1	mass					1513:1516	total fat mass	1503:1516	total fat mass (r = 0.3, p = 0.01)	1503:1536	However, trunk lean mass (p = 0.0375) and total lean mass (p = 0.038) were higher in the PRO group compared to the CHO group at 18 months There were also significant associations for GL and change in total fat mass (r = 0.3, p = 0.01), change in BMI (r = 0.3, p = 0.005), and change in the lean-to-fat mass ratio (r = -0.3, p = 0.002).
28492492	1	30	theme	inflammation	324:335	arg1	intermediary					380:391	intermediary	380:391	intermediary	380:391	Controversy exists as to whether high glycemic index/glycemic load (GI/GL) diets increase the risk of chronic inflammation, which has been postulated as a pathogenic intermediary between such diets and age-related alterations in body composition and insulin resistance.
28492492	1	30	theme	inflammation	324:335	arg1	risk					308:311	the risk	304:311	the risk	304:311	Controversy exists as to whether high glycemic index/glycemic load (GI/GL) diets increase the risk of chronic inflammation, which has been postulated as a pathogenic intermediary between such diets and age-related alterations in body composition and insulin resistance.
28492492	0	31	theme	Resistance	78:87	arg1	Markers					45:51	Markers	45:51	Markers of Inflammation, Insulin Resistance, and Body Composition in Postmenopausal American Women	45:142	The Effect of Dietary Glycemic Properties on Markers of Inflammation, Insulin Resistance, and Body Composition in Postmenopausal American Women: An Ancillary Study from a Multicenter Protein Supplementation Trial.
28492492	6	32	theme	total	1345:1349	arg1	0.038					1366:1370	p = 0.038	1362:1370	p = 0.038	1362:1370	However, trunk lean mass (p = 0.0375) and total lean mass (p = 0.038) were higher in the PRO group compared to the CHO group at 18 months There were also significant associations for GL and change in total fat mass (r = 0.3, p = 0.01), change in BMI (r = 0.3, p = 0.005), and change in the lean-to-fat mass ratio (r = -0.3, p = 0.002).
28492492	6	32	theme	total	1345:1349	arg1	mass					1356:1359	total lean mass	1345:1359	total lean mass (p = 0.038)	1345:1371	However, trunk lean mass (p = 0.0375) and total lean mass (p = 0.038) were higher in the PRO group compared to the CHO group at 18 months There were also significant associations for GL and change in total fat mass (r = 0.3, p = 0.01), change in BMI (r = 0.3, p = 0.005), and change in the lean-to-fat mass ratio (r = -0.3, p = 0.002).
28492492	2	33	theme	=	611:611	arg1	n					609:609	n = 38	609:614	n = 38	609:614	We conducted an ancillary study to a randomized, double-blind trial comparing the effects of a whey protein supplement (PRO, n = 38) and a maltodextrin supplement (CHO, n = 46) on bone density to evaluate the impact of a calibrated increase in GI/GL on inflammation, insulin resistance, and body composition in a healthy aging population.
28492492	0	34	from	Women	138:142	arg1	Markers					45:51	Markers	45:51	Markers of Inflammation, Insulin Resistance, and Body Composition in Postmenopausal American Women	45:142	The Effect of Dietary Glycemic Properties on Markers of Inflammation, Insulin Resistance, and Body Composition in Postmenopausal American Women: An Ancillary Study from a Multicenter Protein Supplementation Trial.
28492492	2	35	theme	supplement	636:645	arg1	effects					566:572	the effects	562:572	the effects of a whey protein supplement (PRO, n = 38) and a maltodextrin supplement (CHO, n = 46) on bone density	562:675	We conducted an ancillary study to a randomized, double-blind trial comparing the effects of a whey protein supplement (PRO, n = 38) and a maltodextrin supplement (CHO, n = 46) on bone density to evaluate the impact of a calibrated increase in GI/GL on inflammation, insulin resistance, and body composition in a healthy aging population.
28492492	1	36	theme	load	276:279	arg1	diets					289:293	high glycemic index/glycemic load (GI/GL) diets	247:293	high glycemic index/glycemic load (GI/GL) diets	247:293	Controversy exists as to whether high glycemic index/glycemic load (GI/GL) diets increase the risk of chronic inflammation, which has been postulated as a pathogenic intermediary between such diets and age-related alterations in body composition and insulin resistance.
28492492	6	37	theme	=	1619:1619	arg1	r					1617:1617	r	1617:1617	r = -0.3	1617:1624	However, trunk lean mass (p = 0.0375) and total lean mass (p = 0.038) were higher in the PRO group compared to the CHO group at 18 months There were also significant associations for GL and change in total fat mass (r = 0.3, p = 0.01), change in BMI (r = 0.3, p = 0.005), and change in the lean-to-fat mass ratio (r = -0.3, p = 0.002).
28492492	6	38	theme	=	1331:1331	arg1	p					1329:1329	p = 0.0375	1329:1338	p = 0.0375	1329:1338	However, trunk lean mass (p = 0.0375) and total lean mass (p = 0.038) were higher in the PRO group compared to the CHO group at 18 months There were also significant associations for GL and change in total fat mass (r = 0.3, p = 0.01), change in BMI (r = 0.3, p = 0.005), and change in the lean-to-fat mass ratio (r = -0.3, p = 0.002).
28492492	6	38	theme	=	1331:1331	arg1	mass					1323:1326	trunk lean mass	1312:1326	trunk lean mass (p = 0.0375)	1312:1339	However, trunk lean mass (p = 0.0375) and total lean mass (p = 0.038) were higher in the PRO group compared to the CHO group at 18 months There were also significant associations for GL and change in total fat mass (r = 0.3, p = 0.01), change in BMI (r = 0.3, p = 0.005), and change in the lean-to-fat mass ratio (r = -0.3, p = 0.002).
28492492	3	39	theme	HOMA	848:851	arg1	Markers					823:829	Markers	823:829	Markers of inflammation, HOMA, body composition, and GI/GL (estimated from 3-day food records)	823:916	Markers of inflammation, HOMA, body composition, and GI/GL (estimated from 3-day food records) were assessed at baseline and 18 months.
28492492	6	40	theme	significant	1457:1467	arg1	associations					1469:1480	significant associations	1457:1480	significant associations for GL and change in total fat mass (r = 0.3, p = 0.01), change in BMI (r = 0.3, p = 0.005), and change in the lean-to-fat mass ratio (r = -0.3, p = 0.002)	1457:1636	However, trunk lean mass (p = 0.0375) and total lean mass (p = 0.038) were higher in the PRO group compared to the CHO group at 18 months There were also significant associations for GL and change in total fat mass (r = 0.3, p = 0.01), change in BMI (r = 0.3, p = 0.005), and change in the lean-to-fat mass ratio (r = -0.3, p = 0.002).
28492492	2	41	from	resistance	759:768	arg1	population					811:820	a healthy aging population	795:820	a healthy aging population	795:820	We conducted an ancillary study to a randomized, double-blind trial comparing the effects of a whey protein supplement (PRO, n = 38) and a maltodextrin supplement (CHO, n = 46) on bone density to evaluate the impact of a calibrated increase in GI/GL on inflammation, insulin resistance, and body composition in a healthy aging population.
28492492	3	42	theme	inflammation	834:845	arg1	Markers					823:829	Markers	823:829	Markers of inflammation, HOMA, body composition, and GI/GL (estimated from 3-day food records)	823:916	Markers of inflammation, HOMA, body composition, and GI/GL (estimated from 3-day food records) were assessed at baseline and 18 months.
28492492	4	43	theme	±	1082:1082	arg1	±					1095:1095	86.5 ± 4.1 → 82.0 ± 3.6	1077:1099	86.5 ± 4.1 → 82.0 ± 3.6 (p < 0.0001)	1077:1112	By 18 months, the GL in the CHO group increased by 34%, 88.4 ± 5.2 → 118.5 ± 4.9 and did not change in the PRO group, 86.5 ± 4.1 → 82.0 ± 3.6 (p < 0.0001).
28492492	4	43	theme	±	1082:1082	arg1	p					1102:1102	p < 0.0001	1102:1111	p < 0.0001	1102:1111	By 18 months, the GL in the CHO group increased by 34%, 88.4 ± 5.2 → 118.5 ± 4.9 and did not change in the PRO group, 86.5 ± 4.1 → 82.0 ± 3.6 (p < 0.0001).
28492492	4	43	theme	±	1082:1082	arg1	group					1070:1074	the PRO group	1062:1074	the PRO group	1062:1074	By 18 months, the GL in the CHO group increased by 34%, 88.4 ± 5.2 → 118.5 ± 4.9 and did not change in the PRO group, 86.5 ± 4.1 → 82.0 ± 3.6 (p < 0.0001).
28492492	0	44	theme	Glycemic	22:29	arg1	Properties					31:40	Dietary Glycemic Properties	14:40	Dietary Glycemic Properties	14:40	The Effect of Dietary Glycemic Properties on Markers of Inflammation, Insulin Resistance, and Body Composition in Postmenopausal American Women: An Ancillary Study from a Multicenter Protein Supplementation Trial.
28492492	0	45	from	Trial	207:211	arg1	Study					158:162	An Ancillary Study	145:162	The Effect of Dietary Glycemic Properties on Markers of Inflammation, Insulin Resistance, and Body Composition in Postmenopausal American Women: An Ancillary Study from a Multicenter Protein Supplementation Trial.	0:212	The Effect of Dietary Glycemic Properties on Markers of Inflammation, Insulin Resistance, and Body Composition in Postmenopausal American Women: An Ancillary Study from a Multicenter Protein Supplementation Trial.
28492492	4	46	theme	→	1088:1088	arg1	±					1095:1095	86.5 ± 4.1 → 82.0 ± 3.6	1077:1099	86.5 ± 4.1 → 82.0 ± 3.6 (p < 0.0001)	1077:1112	By 18 months, the GL in the CHO group increased by 34%, 88.4 ± 5.2 → 118.5 ± 4.9 and did not change in the PRO group, 86.5 ± 4.1 → 82.0 ± 3.6 (p < 0.0001).
28492492	4	46	theme	→	1088:1088	arg1	p					1102:1102	p < 0.0001	1102:1111	p < 0.0001	1102:1111	By 18 months, the GL in the CHO group increased by 34%, 88.4 ± 5.2 → 118.5 ± 4.9 and did not change in the PRO group, 86.5 ± 4.1 → 82.0 ± 3.6 (p < 0.0001).
28492492	4	46	theme	→	1088:1088	arg1	group					1070:1074	the PRO group	1062:1074	the PRO group	1062:1074	By 18 months, the GL in the CHO group increased by 34%, 88.4 ± 5.2 → 118.5 ± 4.9 and did not change in the PRO group, 86.5 ± 4.1 → 82.0 ± 3.6 (p < 0.0001).
28492492	3	47	theme	body	854:857	arg1	composition					859:869	body composition	854:869	body composition	854:869	Markers of inflammation, HOMA, body composition, and GI/GL (estimated from 3-day food records) were assessed at baseline and 18 months.
28492492	5	48	theme	significant	1241:1251	arg1	associations					1253:1264	there significant associations	1235:1264	there significant associations between GL and inflammatory markers	1235:1300	Despite this change there were no differences in serum CRP, IL-6, or HOMA at 18 months between the two groups, nor were there significant associations between GL and inflammatory markers.
28492492	4	49	theme	PRO	1066:1068	arg1	±					1095:1095	86.5 ± 4.1 → 82.0 ± 3.6	1077:1099	86.5 ± 4.1 → 82.0 ± 3.6 (p < 0.0001)	1077:1112	By 18 months, the GL in the CHO group increased by 34%, 88.4 ± 5.2 → 118.5 ± 4.9 and did not change in the PRO group, 86.5 ± 4.1 → 82.0 ± 3.6 (p < 0.0001).
28492492	4	49	theme	PRO	1066:1068	arg1	group					1070:1074	the PRO group	1062:1074	the PRO group	1062:1074	By 18 months, the GL in the CHO group increased by 34%, 88.4 ± 5.2 → 118.5 ± 4.9 and did not change in the PRO group, 86.5 ± 4.1 → 82.0 ± 3.6 (p < 0.0001).
28492492	6	50	theme	lean-to-fat	1593:1603	arg1	ratio					1610:1614	the lean-to-fat mass ratio	1589:1614	the lean-to-fat mass ratio (r = -0.3, p = 0.002)	1589:1636	However, trunk lean mass (p = 0.0375) and total lean mass (p = 0.038) were higher in the PRO group compared to the CHO group at 18 months There were also significant associations for GL and change in total fat mass (r = 0.3, p = 0.01), change in BMI (r = 0.3, p = 0.005), and change in the lean-to-fat mass ratio (r = -0.3, p = 0.002).
28492492	7	51	from	change	1735:1740	arg1	markers					1745:1751	markers	1745:1751	markers of inflammation or insulin resistance	1745:1789	Our data suggest that as dietary GL increases within the moderate range, there is no detectable change in markers of inflammation or insulin resistance, despite which there is a negative effect on body composition.
28492492	2	52	theme	body	775:778	arg1	composition					780:790	body composition	775:790	body composition	775:790	We conducted an ancillary study to a randomized, double-blind trial comparing the effects of a whey protein supplement (PRO, n = 38) and a maltodextrin supplement (CHO, n = 46) on bone density to evaluate the impact of a calibrated increase in GI/GL on inflammation, insulin resistance, and body composition in a healthy aging population.
28492492	3	53	theme	GI/GL	876:880	arg1	Markers					823:829	Markers	823:829	Markers of inflammation, HOMA, body composition, and GI/GL (estimated from 3-day food records)	823:916	Markers of inflammation, HOMA, body composition, and GI/GL (estimated from 3-day food records) were assessed at baseline and 18 months.
28492492	0	54	from	Composition	99:109	arg1	Women					138:142	Postmenopausal American Women	114:142	Postmenopausal American Women	114:142	The Effect of Dietary Glycemic Properties on Markers of Inflammation, Insulin Resistance, and Body Composition in Postmenopausal American Women: An Ancillary Study from a Multicenter Protein Supplementation Trial.
28492492	4	55	theme	<	1104:1104	arg1	±					1095:1095	86.5 ± 4.1 → 82.0 ± 3.6	1077:1099	86.5 ± 4.1 → 82.0 ± 3.6 (p < 0.0001)	1077:1112	By 18 months, the GL in the CHO group increased by 34%, 88.4 ± 5.2 → 118.5 ± 4.9 and did not change in the PRO group, 86.5 ± 4.1 → 82.0 ± 3.6 (p < 0.0001).
28492492	4	55	theme	<	1104:1104	arg1	p					1102:1102	p < 0.0001	1102:1111	p < 0.0001	1102:1111	By 18 months, the GL in the CHO group increased by 34%, 88.4 ± 5.2 → 118.5 ± 4.9 and did not change in the PRO group, 86.5 ± 4.1 → 82.0 ± 3.6 (p < 0.0001).
28492492	7	56	theme	negative	1817:1824	arg1	effect					1826:1831	a negative effect	1815:1831	a negative effect on body composition	1815:1851	Our data suggest that as dietary GL increases within the moderate range, there is no detectable change in markers of inflammation or insulin resistance, despite which there is a negative effect on body composition.
28492492	2	57	theme	protein	584:590	arg1	PRO					604:606	PRO	604:606	PRO	604:606	We conducted an ancillary study to a randomized, double-blind trial comparing the effects of a whey protein supplement (PRO, n = 38) and a maltodextrin supplement (CHO, n = 46) on bone density to evaluate the impact of a calibrated increase in GI/GL on inflammation, insulin resistance, and body composition in a healthy aging population.
28492492	2	57	theme	protein	584:590	arg1	supplement					592:601	a whey protein supplement	577:601	a whey protein supplement (PRO, n = 38)	577:615	We conducted an ancillary study to a randomized, double-blind trial comparing the effects of a whey protein supplement (PRO, n = 38) and a maltodextrin supplement (CHO, n = 46) on bone density to evaluate the impact of a calibrated increase in GI/GL on inflammation, insulin resistance, and body composition in a healthy aging population.
28492492	1	58	from	alterations	428:438	arg1	composition					448:458	body composition	443:458	body composition	443:458	Controversy exists as to whether high glycemic index/glycemic load (GI/GL) diets increase the risk of chronic inflammation, which has been postulated as a pathogenic intermediary between such diets and age-related alterations in body composition and insulin resistance.
28492492	1	58	from	alterations	428:438	arg1	resistance					472:481	insulin resistance	464:481	insulin resistance	464:481	Controversy exists as to whether high glycemic index/glycemic load (GI/GL) diets increase the risk of chronic inflammation, which has been postulated as a pathogenic intermediary between such diets and age-related alterations in body composition and insulin resistance.
28492492	2	59	theme	healthy	797:803	arg1	population					811:820	a healthy aging population	795:820	a healthy aging population	795:820	We conducted an ancillary study to a randomized, double-blind trial comparing the effects of a whey protein supplement (PRO, n = 38) and a maltodextrin supplement (CHO, n = 46) on bone density to evaluate the impact of a calibrated increase in GI/GL on inflammation, insulin resistance, and body composition in a healthy aging population.
28492492	0	60	theme	Multicenter	171:181	arg1	Trial					207:211	a Multicenter Protein Supplementation Trial	169:211	a Multicenter Protein Supplementation Trial	169:211	The Effect of Dietary Glycemic Properties on Markers of Inflammation, Insulin Resistance, and Body Composition in Postmenopausal American Women: An Ancillary Study from a Multicenter Protein Supplementation Trial.
28492492	1	61	theme	glycemic	252:259	arg1	GI/GL					282:286	GI/GL	282:286	GI/GL	282:286	Controversy exists as to whether high glycemic index/glycemic load (GI/GL) diets increase the risk of chronic inflammation, which has been postulated as a pathogenic intermediary between such diets and age-related alterations in body composition and insulin resistance.
28492492	1	61	theme	glycemic	252:259	arg1	load					276:279	glycemic index/glycemic load	252:279	high glycemic index/glycemic load (GI/GL) diets	247:293	Controversy exists as to whether high glycemic index/glycemic load (GI/GL) diets increase the risk of chronic inflammation, which has been postulated as a pathogenic intermediary between such diets and age-related alterations in body composition and insulin resistance.
28492492	2	62	dep	randomized	521:530	arg1	double-blind					533:544	double-blind	533:544	double-blind	533:544	We conducted an ancillary study to a randomized, double-blind trial comparing the effects of a whey protein supplement (PRO, n = 38) and a maltodextrin supplement (CHO, n = 46) on bone density to evaluate the impact of a calibrated increase in GI/GL on inflammation, insulin resistance, and body composition in a healthy aging population.
28492492	3	63	theme	3-day	898:902	arg1	records					909:915	3-day food records	898:915	3-day food records	898:915	Markers of inflammation, HOMA, body composition, and GI/GL (estimated from 3-day food records) were assessed at baseline and 18 months.
28492492	2	64	from	effects	566:572	arg1	density					669:675	bone density	664:675	bone density	664:675	We conducted an ancillary study to a randomized, double-blind trial comparing the effects of a whey protein supplement (PRO, n = 38) and a maltodextrin supplement (CHO, n = 46) on bone density to evaluate the impact of a calibrated increase in GI/GL on inflammation, insulin resistance, and body composition in a healthy aging population.
28492492	6	65	from	change	1579:1584	arg1	ratio					1610:1614	the lean-to-fat mass ratio	1589:1614	the lean-to-fat mass ratio (r = -0.3, p = 0.002)	1589:1636	However, trunk lean mass (p = 0.0375) and total lean mass (p = 0.038) were higher in the PRO group compared to the CHO group at 18 months There were also significant associations for GL and change in total fat mass (r = 0.3, p = 0.01), change in BMI (r = 0.3, p = 0.005), and change in the lean-to-fat mass ratio (r = -0.3, p = 0.002).
28492492	6	65	from	change	1579:1584	arg1	BMI					1549:1551	BMI	1549:1551	BMI (r = 0.3, p = 0.005)	1549:1572	However, trunk lean mass (p = 0.0375) and total lean mass (p = 0.038) were higher in the PRO group compared to the CHO group at 18 months There were also significant associations for GL and change in total fat mass (r = 0.3, p = 0.01), change in BMI (r = 0.3, p = 0.005), and change in the lean-to-fat mass ratio (r = -0.3, p = 0.002).
28492492	6	65	from	change	1579:1584	arg1	mass					1513:1516	total fat mass	1503:1516	total fat mass (r = 0.3, p = 0.01)	1503:1536	However, trunk lean mass (p = 0.0375) and total lean mass (p = 0.038) were higher in the PRO group compared to the CHO group at 18 months There were also significant associations for GL and change in total fat mass (r = 0.3, p = 0.01), change in BMI (r = 0.3, p = 0.005), and change in the lean-to-fat mass ratio (r = -0.3, p = 0.002).
28492492	0	66	theme	Supplementation	191:205	arg1	Trial					207:211	a Multicenter Protein Supplementation Trial	169:211	a Multicenter Protein Supplementation Trial	169:211	The Effect of Dietary Glycemic Properties on Markers of Inflammation, Insulin Resistance, and Body Composition in Postmenopausal American Women: An Ancillary Study from a Multicenter Protein Supplementation Trial.
28492492	1	67	theme	body	443:446	arg1	composition					448:458	body composition	443:458	body composition	443:458	Controversy exists as to whether high glycemic index/glycemic load (GI/GL) diets increase the risk of chronic inflammation, which has been postulated as a pathogenic intermediary between such diets and age-related alterations in body composition and insulin resistance.
28492492	6	68	dep	mass	1513:1516	arg1	=					1530:1530	=	1530:1530	=	1530:1530	However, trunk lean mass (p = 0.0375) and total lean mass (p = 0.038) were higher in the PRO group compared to the CHO group at 18 months There were also significant associations for GL and change in total fat mass (r = 0.3, p = 0.01), change in BMI (r = 0.3, p = 0.005), and change in the lean-to-fat mass ratio (r = -0.3, p = 0.002).
28492492	6	69	dep	=	1521:1521	arg1	0.3					1523:1525	0.3	1523:1525	0.3	1523:1525	However, trunk lean mass (p = 0.0375) and total lean mass (p = 0.038) were higher in the PRO group compared to the CHO group at 18 months There were also significant associations for GL and change in total fat mass (r = 0.3, p = 0.01), change in BMI (r = 0.3, p = 0.005), and change in the lean-to-fat mass ratio (r = -0.3, p = 0.002).
28492492	2	70	theme	calibrated	705:714	arg1	increase					716:723	a calibrated increase	703:723	a calibrated increase in GI/GL	703:732	We conducted an ancillary study to a randomized, double-blind trial comparing the effects of a whey protein supplement (PRO, n = 38) and a maltodextrin supplement (CHO, n = 46) on bone density to evaluate the impact of a calibrated increase in GI/GL on inflammation, insulin resistance, and body composition in a healthy aging population.
28492492	7	71	theme	dietary	1664:1670	arg1	GL					1672:1673	dietary GL	1664:1673	dietary GL	1664:1673	Our data suggest that as dietary GL increases within the moderate range, there is no detectable change in markers of inflammation or insulin resistance, despite which there is a negative effect on body composition.
28492492	6	72	dep	=	1530:1530	arg1	r					1519:1519	r	1519:1519	r = 0.3	1519:1525	However, trunk lean mass (p = 0.0375) and total lean mass (p = 0.038) were higher in the PRO group compared to the CHO group at 18 months There were also significant associations for GL and change in total fat mass (r = 0.3, p = 0.01), change in BMI (r = 0.3, p = 0.005), and change in the lean-to-fat mass ratio (r = -0.3, p = 0.002).
28492492	6	73	theme	=	1521:1521	arg1	r					1519:1519	r	1519:1519	r = 0.3	1519:1525	However, trunk lean mass (p = 0.0375) and total lean mass (p = 0.038) were higher in the PRO group compared to the CHO group at 18 months There were also significant associations for GL and change in total fat mass (r = 0.3, p = 0.01), change in BMI (r = 0.3, p = 0.005), and change in the lean-to-fat mass ratio (r = -0.3, p = 0.002).
28492492	0	74	theme	American	129:136	arg1	Women					138:142	Postmenopausal American Women	114:142	Postmenopausal American Women	114:142	The Effect of Dietary Glycemic Properties on Markers of Inflammation, Insulin Resistance, and Body Composition in Postmenopausal American Women: An Ancillary Study from a Multicenter Protein Supplementation Trial.
28492492	4	75	from	GL	977:978	arg1	group					991:995	the CHO group	983:995	the CHO group	983:995	By 18 months, the GL in the CHO group increased by 34%, 88.4 ± 5.2 → 118.5 ± 4.9 and did not change in the PRO group, 86.5 ± 4.1 → 82.0 ± 3.6 (p < 0.0001).
28492492	2	76	theme	randomized	521:530	arg1	trial					546:550	a randomized, double-blind trial	519:550	a randomized, double-blind trial comparing the effects of a whey protein supplement (PRO, n = 38) and a maltodextrin supplement (CHO, n = 46) on bone density to evaluate the impact of a calibrated increase in GI/GL on inflammation, insulin resistance, and body composition in a healthy aging population	519:820	We conducted an ancillary study to a randomized, double-blind trial comparing the effects of a whey protein supplement (PRO, n = 38) and a maltodextrin supplement (CHO, n = 46) on bone density to evaluate the impact of a calibrated increase in GI/GL on inflammation, insulin resistance, and body composition in a healthy aging population.
28492492	0	77	from	Inflammation	56:67	arg1	Women					138:142	Postmenopausal American Women	114:142	Postmenopausal American Women	114:142	The Effect of Dietary Glycemic Properties on Markers of Inflammation, Insulin Resistance, and Body Composition in Postmenopausal American Women: An Ancillary Study from a Multicenter Protein Supplementation Trial.
28492492	7	78	theme	moderate	1696:1703	arg1	range					1705:1709	the moderate range	1692:1709	the moderate range	1692:1709	Our data suggest that as dietary GL increases within the moderate range, there is no detectable change in markers of inflammation or insulin resistance, despite which there is a negative effect on body composition.
28492492	2	79	theme	=	655:655	arg1	n					653:653	n = 46	653:658	n = 46	653:658	We conducted an ancillary study to a randomized, double-blind trial comparing the effects of a whey protein supplement (PRO, n = 38) and a maltodextrin supplement (CHO, n = 46) on bone density to evaluate the impact of a calibrated increase in GI/GL on inflammation, insulin resistance, and body composition in a healthy aging population.
28492492	7	80	theme	body	1836:1839	arg1	composition					1841:1851	body composition	1836:1851	body composition	1836:1851	Our data suggest that as dietary GL increases within the moderate range, there is no detectable change in markers of inflammation or insulin resistance, despite which there is a negative effect on body composition.
28492492	0	81	theme	Ancillary	148:156	arg1	Study					158:162	An Ancillary Study	145:162	The Effect of Dietary Glycemic Properties on Markers of Inflammation, Insulin Resistance, and Body Composition in Postmenopausal American Women: An Ancillary Study from a Multicenter Protein Supplementation Trial.	0:212	The Effect of Dietary Glycemic Properties on Markers of Inflammation, Insulin Resistance, and Body Composition in Postmenopausal American Women: An Ancillary Study from a Multicenter Protein Supplementation Trial.
28492492	1	82	theme	such	401:404	arg1	diets					406:410	such diets	401:410	such diets	401:410	Controversy exists as to whether high glycemic index/glycemic load (GI/GL) diets increase the risk of chronic inflammation, which has been postulated as a pathogenic intermediary between such diets and age-related alterations in body composition and insulin resistance.
28492492	6	83	theme	trunk	1312:1316	arg1	p					1329:1329	p = 0.0375	1329:1338	p = 0.0375	1329:1338	However, trunk lean mass (p = 0.0375) and total lean mass (p = 0.038) were higher in the PRO group compared to the CHO group at 18 months There were also significant associations for GL and change in total fat mass (r = 0.3, p = 0.01), change in BMI (r = 0.3, p = 0.005), and change in the lean-to-fat mass ratio (r = -0.3, p = 0.002).
28492492	6	83	theme	trunk	1312:1316	arg1	mass					1323:1326	trunk lean mass	1312:1326	trunk lean mass (p = 0.0375)	1312:1339	However, trunk lean mass (p = 0.0375) and total lean mass (p = 0.038) were higher in the PRO group compared to the CHO group at 18 months There were also significant associations for GL and change in total fat mass (r = 0.3, p = 0.01), change in BMI (r = 0.3, p = 0.005), and change in the lean-to-fat mass ratio (r = -0.3, p = 0.002).
28492492	6	84	theme	p	1362:1362	arg1	0.038					1366:1370	p = 0.038	1362:1370	p = 0.038	1362:1370	However, trunk lean mass (p = 0.0375) and total lean mass (p = 0.038) were higher in the PRO group compared to the CHO group at 18 months There were also significant associations for GL and change in total fat mass (r = 0.3, p = 0.01), change in BMI (r = 0.3, p = 0.005), and change in the lean-to-fat mass ratio (r = -0.3, p = 0.002).
28492492	6	84	theme	p	1362:1362	arg1	mass					1356:1359	total lean mass	1345:1359	total lean mass (p = 0.038)	1345:1371	However, trunk lean mass (p = 0.0375) and total lean mass (p = 0.038) were higher in the PRO group compared to the CHO group at 18 months There were also significant associations for GL and change in total fat mass (r = 0.3, p = 0.01), change in BMI (r = 0.3, p = 0.005), and change in the lean-to-fat mass ratio (r = -0.3, p = 0.002).
28492492	6	85	dep	BMI	1549:1551	arg1	=					1565:1565	=	1565:1565	=	1565:1565	However, trunk lean mass (p = 0.0375) and total lean mass (p = 0.038) were higher in the PRO group compared to the CHO group at 18 months There were also significant associations for GL and change in total fat mass (r = 0.3, p = 0.01), change in BMI (r = 0.3, p = 0.005), and change in the lean-to-fat mass ratio (r = -0.3, p = 0.002).
28492492	2	86	theme	bone	664:667	arg1	density					669:675	bone density	664:675	bone density	664:675	We conducted an ancillary study to a randomized, double-blind trial comparing the effects of a whey protein supplement (PRO, n = 38) and a maltodextrin supplement (CHO, n = 46) on bone density to evaluate the impact of a calibrated increase in GI/GL on inflammation, insulin resistance, and body composition in a healthy aging population.
28492492	1	87	from	diets	406:410	arg1	composition					448:458	body composition	443:458	body composition	443:458	Controversy exists as to whether high glycemic index/glycemic load (GI/GL) diets increase the risk of chronic inflammation, which has been postulated as a pathogenic intermediary between such diets and age-related alterations in body composition and insulin resistance.
28492492	1	87	from	diets	406:410	arg1	resistance					472:481	insulin resistance	464:481	insulin resistance	464:481	Controversy exists as to whether high glycemic index/glycemic load (GI/GL) diets increase the risk of chronic inflammation, which has been postulated as a pathogenic intermediary between such diets and age-related alterations in body composition and insulin resistance.
28492492	6	88	dep	=	1629:1629	arg1	r					1617:1617	r	1617:1617	r = -0.3	1617:1624	However, trunk lean mass (p = 0.0375) and total lean mass (p = 0.038) were higher in the PRO group compared to the CHO group at 18 months There were also significant associations for GL and change in total fat mass (r = 0.3, p = 0.01), change in BMI (r = 0.3, p = 0.005), and change in the lean-to-fat mass ratio (r = -0.3, p = 0.002).
28492492	0	89	theme	Insulin	70:76	arg1	Resistance					78:87	Insulin Resistance	70:87	Insulin Resistance	70:87	The Effect of Dietary Glycemic Properties on Markers of Inflammation, Insulin Resistance, and Body Composition in Postmenopausal American Women: An Ancillary Study from a Multicenter Protein Supplementation Trial.
28492492	1	90	theme	chronic	316:322	arg1	inflammation					324:335	chronic inflammation	316:335	chronic inflammation	316:335	Controversy exists as to whether high glycemic index/glycemic load (GI/GL) diets increase the risk of chronic inflammation, which has been postulated as a pathogenic intermediary between such diets and age-related alterations in body composition and insulin resistance.
28492492	0	91	from	Effect	4:9	arg1	Markers					45:51	Markers	45:51	Markers of Inflammation, Insulin Resistance, and Body Composition in Postmenopausal American Women	45:142	The Effect of Dietary Glycemic Properties on Markers of Inflammation, Insulin Resistance, and Body Composition in Postmenopausal American Women: An Ancillary Study from a Multicenter Protein Supplementation Trial.
28492492	6	92	theme	lean	1351:1354	arg1	0.038					1366:1370	p = 0.038	1362:1370	p = 0.038	1362:1370	However, trunk lean mass (p = 0.0375) and total lean mass (p = 0.038) were higher in the PRO group compared to the CHO group at 18 months There were also significant associations for GL and change in total fat mass (r = 0.3, p = 0.01), change in BMI (r = 0.3, p = 0.005), and change in the lean-to-fat mass ratio (r = -0.3, p = 0.002).
28492492	6	92	theme	lean	1351:1354	arg1	mass					1356:1359	total lean mass	1345:1359	total lean mass (p = 0.038)	1345:1371	However, trunk lean mass (p = 0.0375) and total lean mass (p = 0.038) were higher in the PRO group compared to the CHO group at 18 months There were also significant associations for GL and change in total fat mass (r = 0.3, p = 0.01), change in BMI (r = 0.3, p = 0.005), and change in the lean-to-fat mass ratio (r = -0.3, p = 0.002).
28492492	0	93	from	Markers	45:51	arg1	Women					138:142	Postmenopausal American Women	114:142	Postmenopausal American Women	114:142	The Effect of Dietary Glycemic Properties on Markers of Inflammation, Insulin Resistance, and Body Composition in Postmenopausal American Women: An Ancillary Study from a Multicenter Protein Supplementation Trial.
28492492	7	94	theme	insulin	1772:1778	arg1	resistance					1780:1789	insulin resistance	1772:1789	insulin resistance	1772:1789	Our data suggest that as dietary GL increases within the moderate range, there is no detectable change in markers of inflammation or insulin resistance, despite which there is a negative effect on body composition.
28492492	2	95	from	increase	716:723	arg1	GI/GL					728:732	GI/GL	728:732	GI/GL	728:732	We conducted an ancillary study to a randomized, double-blind trial comparing the effects of a whey protein supplement (PRO, n = 38) and a maltodextrin supplement (CHO, n = 46) on bone density to evaluate the impact of a calibrated increase in GI/GL on inflammation, insulin resistance, and body composition in a healthy aging population.
28492492	0	96	theme	Body	94:97	arg1	Composition					99:109	Body Composition	94:109	Body Composition	94:109	The Effect of Dietary Glycemic Properties on Markers of Inflammation, Insulin Resistance, and Body Composition in Postmenopausal American Women: An Ancillary Study from a Multicenter Protein Supplementation Trial.
28492492	2	97	theme	ancillary	500:508	arg1	study					510:514	an ancillary study	497:514	an ancillary study	497:514	We conducted an ancillary study to a randomized, double-blind trial comparing the effects of a whey protein supplement (PRO, n = 38) and a maltodextrin supplement (CHO, n = 46) on bone density to evaluate the impact of a calibrated increase in GI/GL on inflammation, insulin resistance, and body composition in a healthy aging population.
28492492	6	98	theme	PRO	1392:1394	arg1	group					1396:1400	the PRO group	1388:1400	the PRO group	1388:1400	However, trunk lean mass (p = 0.0375) and total lean mass (p = 0.038) were higher in the PRO group compared to the CHO group at 18 months There were also significant associations for GL and change in total fat mass (r = 0.3, p = 0.01), change in BMI (r = 0.3, p = 0.005), and change in the lean-to-fat mass ratio (r = -0.3, p = 0.002).
28492492	2	99	from	GI/GL	728:732	arg1	impact					693:698	the impact	689:698	the impact of a calibrated increase in GI/GL on inflammation, insulin resistance, and body composition in a healthy aging population	689:820	We conducted an ancillary study to a randomized, double-blind trial comparing the effects of a whey protein supplement (PRO, n = 38) and a maltodextrin supplement (CHO, n = 46) on bone density to evaluate the impact of a calibrated increase in GI/GL on inflammation, insulin resistance, and body composition in a healthy aging population.
28492492	7	100	from	effect	1826:1831	arg1	composition					1841:1851	body composition	1836:1851	body composition	1836:1851	Our data suggest that as dietary GL increases within the moderate range, there is no detectable change in markers of inflammation or insulin resistance, despite which there is a negative effect on body composition.
28492492	6	101	dep	=	1565:1565	arg1	r					1554:1554	r	1554:1554	r = 0.3	1554:1560	However, trunk lean mass (p = 0.0375) and total lean mass (p = 0.038) were higher in the PRO group compared to the CHO group at 18 months There were also significant associations for GL and change in total fat mass (r = 0.3, p = 0.01), change in BMI (r = 0.3, p = 0.005), and change in the lean-to-fat mass ratio (r = -0.3, p = 0.002).
28492492	2	102	theme	maltodextrin	623:634	arg1	CHO					648:650	CHO	648:650	CHO	648:650	We conducted an ancillary study to a randomized, double-blind trial comparing the effects of a whey protein supplement (PRO, n = 38) and a maltodextrin supplement (CHO, n = 46) on bone density to evaluate the impact of a calibrated increase in GI/GL on inflammation, insulin resistance, and body composition in a healthy aging population.
28492492	2	102	theme	maltodextrin	623:634	arg1	supplement					636:645	a maltodextrin supplement	621:645	a maltodextrin supplement (CHO, n = 46)	621:659	We conducted an ancillary study to a randomized, double-blind trial comparing the effects of a whey protein supplement (PRO, n = 38) and a maltodextrin supplement (CHO, n = 46) on bone density to evaluate the impact of a calibrated increase in GI/GL on inflammation, insulin resistance, and body composition in a healthy aging population.
28492492	2	103	from	inflammation	737:748	arg1	population					811:820	a healthy aging population	795:820	a healthy aging population	795:820	We conducted an ancillary study to a randomized, double-blind trial comparing the effects of a whey protein supplement (PRO, n = 38) and a maltodextrin supplement (CHO, n = 46) on bone density to evaluate the impact of a calibrated increase in GI/GL on inflammation, insulin resistance, and body composition in a healthy aging population.
28492492	0	104	theme	Dietary	14:20	arg1	Properties					31:40	Dietary Glycemic Properties	14:40	Dietary Glycemic Properties	14:40	The Effect of Dietary Glycemic Properties on Markers of Inflammation, Insulin Resistance, and Body Composition in Postmenopausal American Women: An Ancillary Study from a Multicenter Protein Supplementation Trial.
28492492	2	105	dep	CHO	648:650	arg1	n					653:653	n = 46	653:658	n = 46	653:658	We conducted an ancillary study to a randomized, double-blind trial comparing the effects of a whey protein supplement (PRO, n = 38) and a maltodextrin supplement (CHO, n = 46) on bone density to evaluate the impact of a calibrated increase in GI/GL on inflammation, insulin resistance, and body composition in a healthy aging population.
28492492	5	106	from	differences	1149:1159	arg1	IL-6					1175:1178	IL-6	1175:1178	IL-6	1175:1178	Despite this change there were no differences in serum CRP, IL-6, or HOMA at 18 months between the two groups, nor were there significant associations between GL and inflammatory markers.
28492492	5	106	from	differences	1149:1159	arg1	HOMA					1184:1187	HOMA	1184:1187	HOMA	1184:1187	Despite this change there were no differences in serum CRP, IL-6, or HOMA at 18 months between the two groups, nor were there significant associations between GL and inflammatory markers.
28492492	5	106	from	differences	1149:1159	arg1	months					1195:1200	18 months	1192:1200	18 months	1192:1200	Despite this change there were no differences in serum CRP, IL-6, or HOMA at 18 months between the two groups, nor were there significant associations between GL and inflammatory markers.
28492492	5	106	from	differences	1149:1159	arg1	CRP					1170:1172	serum CRP	1164:1172	serum CRP	1164:1172	Despite this change there were no differences in serum CRP, IL-6, or HOMA at 18 months between the two groups, nor were there significant associations between GL and inflammatory markers.
28492492	0	107	theme	Properties	31:40	arg1	Effect					4:9	The Effect	0:9	The Effect of Dietary Glycemic Properties on Markers of Inflammation, Insulin Resistance, and Body Composition in Postmenopausal American Women: An Ancillary Study from a Multicenter Protein Supplementation Trial.	0:212	The Effect of Dietary Glycemic Properties on Markers of Inflammation, Insulin Resistance, and Body Composition in Postmenopausal American Women: An Ancillary Study from a Multicenter Protein Supplementation Trial.
28492492	6	108	theme	fat	1509:1511	arg1	mass					1513:1516	total fat mass	1503:1516	total fat mass (r = 0.3, p = 0.01)	1503:1536	However, trunk lean mass (p = 0.0375) and total lean mass (p = 0.038) were higher in the PRO group compared to the CHO group at 18 months There were also significant associations for GL and change in total fat mass (r = 0.3, p = 0.01), change in BMI (r = 0.3, p = 0.005), and change in the lean-to-fat mass ratio (r = -0.3, p = 0.002).
28492492	5	109	theme	inflammatory	1281:1292	arg1	markers					1294:1300	inflammatory markers	1281:1300	inflammatory markers	1281:1300	Despite this change there were no differences in serum CRP, IL-6, or HOMA at 18 months between the two groups, nor were there significant associations between GL and inflammatory markers.
28492492	6	110	dep	months	1434:1439	arg1	were					1447:1450	were	1447:1450	were also significant associations for GL and change in total fat mass (r = 0.3, p = 0.01), change in BMI (r = 0.3, p = 0.005), and change in the lean-to-fat mass ratio (r = -0.3, p = 0.002)	1447:1636	However, trunk lean mass (p = 0.0375) and total lean mass (p = 0.038) were higher in the PRO group compared to the CHO group at 18 months There were also significant associations for GL and change in total fat mass (r = 0.3, p = 0.01), change in BMI (r = 0.3, p = 0.005), and change in the lean-to-fat mass ratio (r = -0.3, p = 0.002).
28492492	7	111	theme	resistance	1780:1789	arg1	markers					1745:1751	markers	1745:1751	markers of inflammation or insulin resistance	1745:1789	Our data suggest that as dietary GL increases within the moderate range, there is no detectable change in markers of inflammation or insulin resistance, despite which there is a negative effect on body composition.
28492492	6	112	theme	mass	1605:1608	arg1	ratio					1610:1614	the lean-to-fat mass ratio	1589:1614	the lean-to-fat mass ratio (r = -0.3, p = 0.002)	1589:1636	However, trunk lean mass (p = 0.0375) and total lean mass (p = 0.038) were higher in the PRO group compared to the CHO group at 18 months There were also significant associations for GL and change in total fat mass (r = 0.3, p = 0.01), change in BMI (r = 0.3, p = 0.005), and change in the lean-to-fat mass ratio (r = -0.3, p = 0.002).
28492492	3	113	theme	composition	859:869	arg1	Markers					823:829	Markers	823:829	Markers of inflammation, HOMA, body composition, and GI/GL (estimated from 3-day food records)	823:916	Markers of inflammation, HOMA, body composition, and GI/GL (estimated from 3-day food records) were assessed at baseline and 18 months.
28492492	7	114	theme	inflammation	1756:1767	arg1	markers					1745:1751	markers	1745:1751	markers of inflammation or insulin resistance	1745:1789	Our data suggest that as dietary GL increases within the moderate range, there is no detectable change in markers of inflammation or insulin resistance, despite which there is a negative effect on body composition.
28492492	2	115	from	impact	693:698	arg1	GI/GL					728:732	GI/GL	728:732	GI/GL	728:732	We conducted an ancillary study to a randomized, double-blind trial comparing the effects of a whey protein supplement (PRO, n = 38) and a maltodextrin supplement (CHO, n = 46) on bone density to evaluate the impact of a calibrated increase in GI/GL on inflammation, insulin resistance, and body composition in a healthy aging population.
28492492	2	115	from	impact	693:698	arg1	composition					780:790	body composition	775:790	body composition	775:790	We conducted an ancillary study to a randomized, double-blind trial comparing the effects of a whey protein supplement (PRO, n = 38) and a maltodextrin supplement (CHO, n = 46) on bone density to evaluate the impact of a calibrated increase in GI/GL on inflammation, insulin resistance, and body composition in a healthy aging population.
28492492	2	115	from	impact	693:698	arg1	inflammation					737:748	inflammation	737:748	inflammation	737:748	We conducted an ancillary study to a randomized, double-blind trial comparing the effects of a whey protein supplement (PRO, n = 38) and a maltodextrin supplement (CHO, n = 46) on bone density to evaluate the impact of a calibrated increase in GI/GL on inflammation, insulin resistance, and body composition in a healthy aging population.
28492492	2	115	from	impact	693:698	arg1	resistance					759:768	insulin resistance	751:768	insulin resistance	751:768	We conducted an ancillary study to a randomized, double-blind trial comparing the effects of a whey protein supplement (PRO, n = 38) and a maltodextrin supplement (CHO, n = 46) on bone density to evaluate the impact of a calibrated increase in GI/GL on inflammation, insulin resistance, and body composition in a healthy aging population.
28492492	0	116	dep	Effect	4:9	arg1	Study					158:162	An Ancillary Study	145:162	The Effect of Dietary Glycemic Properties on Markers of Inflammation, Insulin Resistance, and Body Composition in Postmenopausal American Women: An Ancillary Study from a Multicenter Protein Supplementation Trial.	0:212	The Effect of Dietary Glycemic Properties on Markers of Inflammation, Insulin Resistance, and Body Composition in Postmenopausal American Women: An Ancillary Study from a Multicenter Protein Supplementation Trial.
28492492	6	117	dep	ratio	1610:1614	arg1	=					1629:1629	=	1629:1629	=	1629:1629	However, trunk lean mass (p = 0.0375) and total lean mass (p = 0.038) were higher in the PRO group compared to the CHO group at 18 months There were also significant associations for GL and change in total fat mass (r = 0.3, p = 0.01), change in BMI (r = 0.3, p = 0.005), and change in the lean-to-fat mass ratio (r = -0.3, p = 0.002).
28492492	2	118	theme	increase	716:723	arg1	impact					693:698	the impact	689:698	the impact of a calibrated increase in GI/GL on inflammation, insulin resistance, and body composition in a healthy aging population	689:820	We conducted an ancillary study to a randomized, double-blind trial comparing the effects of a whey protein supplement (PRO, n = 38) and a maltodextrin supplement (CHO, n = 46) on bone density to evaluate the impact of a calibrated increase in GI/GL on inflammation, insulin resistance, and body composition in a healthy aging population.
28492492	6	119	theme	total	1503:1507	arg1	mass					1513:1516	total fat mass	1503:1516	total fat mass (r = 0.3, p = 0.01)	1503:1536	However, trunk lean mass (p = 0.0375) and total lean mass (p = 0.038) were higher in the PRO group compared to the CHO group at 18 months There were also significant associations for GL and change in total fat mass (r = 0.3, p = 0.01), change in BMI (r = 0.3, p = 0.005), and change in the lean-to-fat mass ratio (r = -0.3, p = 0.002).
25192854	4	0	theme	first	598:602	arg1	days					607:610	the first 30 days	594:610	the first 30 days of degradation	594:625	Test results of mass loss rate showed that the first 30 days of degradation was mainly starch degradation, and the degradation rate of esterified starch/PLA (ES/PLA) was slower than that of native starch/PLA (NS/PLA).
25192854	4	0	theme	first	598:602	arg1	degradation					645:655	starch degradation	638:655	starch degradation	638:655	Test results of mass loss rate showed that the first 30 days of degradation was mainly starch degradation, and the degradation rate of esterified starch/PLA (ES/PLA) was slower than that of native starch/PLA (NS/PLA).
25192854	3	1	theme	burial	536:541	arg1	method					543:548	soil burial method	531:548	soil burial method	531:548	The influence of the dry method esterification of starch on the degradation characteristics of starch/PLA composites was investigated by the natural aging degradation which was soil burial method.
25192854	3	1	theme	burial	536:541	arg1	degradation					509:519	the natural aging degradation	491:519	the natural aging degradation which was soil burial method	491:548	The influence of the dry method esterification of starch on the degradation characteristics of starch/PLA composites was investigated by the natural aging degradation which was soil burial method.
25192854	7	2	theme	burial	1382:1387	arg1	degradation					1389:1399	soil burial degradation	1377:1399	soil burial degradation	1377:1399	The XRD diffraction peak intensity of PLA in composites showed an increased trend at first which was then followed by a decreased one along with the increasing time of soil burial degradation, indicating that the degradation of amorphous regions of PLA was earlier than its crystalline regions.
25192854	2	3	theme	blending	296:303	arg1	anhydride					312:320	blending maleic anhydride esterified corn starch and PLA	296:351	blending maleic anhydride esterified corn starch and PLA	296:351	Esterified starch/polylactic acid (PLA) biodegradable composite was produced via melt extrusion method with blending maleic anhydride esterified corn starch and PLA.
25192854	7	4	theme	decreased	1329:1337	arg1	one					1339:1341	a decreased one	1327:1341	a decreased one	1327:1341	The XRD diffraction peak intensity of PLA in composites showed an increased trend at first which was then followed by a decreased one along with the increasing time of soil burial degradation, indicating that the degradation of amorphous regions of PLA was earlier than its crystalline regions.
25192854	4	5	theme	degradation	666:676	arg1	slower					721:726	slower	721:726	slower	721:726	Test results of mass loss rate showed that the first 30 days of degradation was mainly starch degradation, and the degradation rate of esterified starch/PLA (ES/PLA) was slower than that of native starch/PLA (NS/PLA).
25192854	4	5	theme	degradation	666:676	arg1	rate					678:681	the degradation rate	662:681	the degradation rate of esterified starch/PLA (ES/PLA)	662:715	Test results of mass loss rate showed that the first 30 days of degradation was mainly starch degradation, and the degradation rate of esterified starch/PLA (ES/PLA) was slower than that of native starch/PLA (NS/PLA).
25192854	6	6	theme	C-H	1179:1181	arg1	intensities					1151:1161	the infrared absorption peak intensities	1122:1161	the infrared absorption peak intensities of C-O, C=O and C-H	1122:1181	With the increasing time of soil burial degradation, the damage degree of NS/PLA and ES/PLA on the exterior and interior were gradually increased, whereas the infrared absorption peak intensities of C-O, C=O and C-H were gradually decreased.
25192854	7	7	theme	increasing	1358:1367	arg1	time					1369:1372	the increasing time	1354:1372	the increasing time of soil burial degradation	1354:1399	The XRD diffraction peak intensity of PLA in composites showed an increased trend at first which was then followed by a decreased one along with the increasing time of soil burial degradation, indicating that the degradation of amorphous regions of PLA was earlier than its crystalline regions.
25192854	6	8	theme	C=O	1171:1173	arg1	intensities					1151:1161	the infrared absorption peak intensities	1122:1161	the infrared absorption peak intensities of C-O, C=O and C-H	1122:1181	With the increasing time of soil burial degradation, the damage degree of NS/PLA and ES/PLA on the exterior and interior were gradually increased, whereas the infrared absorption peak intensities of C-O, C=O and C-H were gradually decreased.
25192854	2	9	theme	corn	333:336	arg1	starch					338:343	esterified corn starch	322:343	esterified corn starch	322:343	Esterified starch/polylactic acid (PLA) biodegradable composite was produced via melt extrusion method with blending maleic anhydride esterified corn starch and PLA.
25192854	10	10	theme	composites	1803:1812	arg1	strength					1770:1777	tensile strength	1762:1777	tensile strength	1762:1777	The tensile strength and bending strength of composites were decreased gradually with soil burial time increasing.
25192854	10	10	theme	composites	1803:1812	arg1	strength					1791:1798	bending strength	1783:1798	bending strength	1783:1798	The tensile strength and bending strength of composites were decreased gradually with soil burial time increasing.
25192854	3	11	from	influence	358:366	arg1	characteristics					430:444	the degradation characteristics	414:444	the degradation characteristics of starch/PLA composites	414:469	The influence of the dry method esterification of starch on the degradation characteristics of starch/PLA composites was investigated by the natural aging degradation which was soil burial method.
25192854	11	12	theme	tensile	1882:1888	arg1	strength					1890:1897	tensile strength	1882:1897	tensile strength	1882:1897	Both the tensile strength and bending strength of ES/PLA were stronger than those of NS/PLA.
25192854	11	13	theme	bending	1903:1909	arg1	strength					1911:1918	bending strength	1903:1918	bending strength	1903:1918	Both the tensile strength and bending strength of ES/PLA were stronger than those of NS/PLA.
25192854	7	14	theme	PLA	1247:1249	arg1	intensity					1234:1242	The XRD diffraction peak intensity	1209:1242	The XRD diffraction peak intensity of PLA in composites	1209:1263	The XRD diffraction peak intensity of PLA in composites showed an increased trend at first which was then followed by a decreased one along with the increasing time of soil burial degradation, indicating that the degradation of amorphous regions of PLA was earlier than its crystalline regions.
25192854	10	15	theme	burial	1849:1854	arg1	time					1856:1859	soil burial time increasing	1844:1870	soil burial time increasing	1844:1870	The tensile strength and bending strength of composites were decreased gradually with soil burial time increasing.
25192854	5	16	theme	ES/PLA	801:806	arg1	smaller					838:844	smaller	838:844	smaller	838:844	Therefore, the damage degree of ES/PLA on the surface and inside was smaller than that of NS/PLA, and the infrared absorption peak intensities of C-O, C=O and C-H were stronger than that of NS/PLA.
25192854	5	16	theme	ES/PLA	801:806	arg1	degree					791:796	the damage degree	780:796	the damage degree of ES/PLA on the surface and inside	780:832	Therefore, the damage degree of ES/PLA on the surface and inside was smaller than that of NS/PLA, and the infrared absorption peak intensities of C-O, C=O and C-H were stronger than that of NS/PLA.
25192854	10	17	dep	strength	1770:1777	arg1	The					1758:1760	The	1758:1760	The	1758:1760	The tensile strength and bending strength of composites were decreased gradually with soil burial time increasing.
25192854	5	18	theme	infrared	875:882	arg1	intensities					900:910	the infrared absorption peak intensities	871:910	the infrared absorption peak intensities of C-O, C=O and C-H	871:930	Therefore, the damage degree of ES/PLA on the surface and inside was smaller than that of NS/PLA, and the infrared absorption peak intensities of C-O, C=O and C-H were stronger than that of NS/PLA.
25192854	5	18	theme	infrared	875:882	arg1	stronger					937:944	stronger	937:944	stronger	937:944	Therefore, the damage degree of ES/PLA on the surface and inside was smaller than that of NS/PLA, and the infrared absorption peak intensities of C-O, C=O and C-H were stronger than that of NS/PLA.
25192854	7	19	theme	increased	1275:1283	arg1	trend					1285:1289	an increased trend	1272:1289	an increased trend at first which was then followed by a decreased one	1272:1341	The XRD diffraction peak intensity of PLA in composites showed an increased trend at first which was then followed by a decreased one along with the increasing time of soil burial degradation, indicating that the degradation of amorphous regions of PLA was earlier than its crystalline regions.
25192854	6	20	theme	peak	1146:1149	arg1	intensities					1151:1161	the infrared absorption peak intensities	1122:1161	the infrared absorption peak intensities of C-O, C=O and C-H	1122:1181	With the increasing time of soil burial degradation, the damage degree of NS/PLA and ES/PLA on the exterior and interior were gradually increased, whereas the infrared absorption peak intensities of C-O, C=O and C-H were gradually decreased.
25192854	2	21	theme	melt	269:272	arg1	method					284:289	melt extrusion method	269:289	melt extrusion method with blending maleic anhydride esterified corn starch and PLA	269:351	Esterified starch/polylactic acid (PLA) biodegradable composite was produced via melt extrusion method with blending maleic anhydride esterified corn starch and PLA.
25192854	5	22	theme	peak	895:898	arg1	intensities					900:910	the infrared absorption peak intensities	871:910	the infrared absorption peak intensities of C-O, C=O and C-H	871:930	Therefore, the damage degree of ES/PLA on the surface and inside was smaller than that of NS/PLA, and the infrared absorption peak intensities of C-O, C=O and C-H were stronger than that of NS/PLA.
25192854	5	22	theme	peak	895:898	arg1	stronger					937:944	stronger	937:944	stronger	937:944	Therefore, the damage degree of ES/PLA on the surface and inside was smaller than that of NS/PLA, and the infrared absorption peak intensities of C-O, C=O and C-H were stronger than that of NS/PLA.
25192854	2	23	with	method	284:289	arg1	anhydride					312:320	blending maleic anhydride esterified corn starch and PLA	296:351	blending maleic anhydride esterified corn starch and PLA	296:351	Esterified starch/polylactic acid (PLA) biodegradable composite was produced via melt extrusion method with blending maleic anhydride esterified corn starch and PLA.
25192854	6	24	theme	infrared	1126:1133	arg1	intensities					1151:1161	the infrared absorption peak intensities	1122:1161	the infrared absorption peak intensities of C-O, C=O and C-H	1122:1181	With the increasing time of soil burial degradation, the damage degree of NS/PLA and ES/PLA on the exterior and interior were gradually increased, whereas the infrared absorption peak intensities of C-O, C=O and C-H were gradually decreased.
25192854	7	25	theme	peak	1229:1232	arg1	intensity					1234:1242	The XRD diffraction peak intensity	1209:1242	The XRD diffraction peak intensity of PLA in composites	1209:1263	The XRD diffraction peak intensity of PLA in composites showed an increased trend at first which was then followed by a decreased one along with the increasing time of soil burial degradation, indicating that the degradation of amorphous regions of PLA was earlier than its crystalline regions.
25192854	4	26	theme	starch/PLA	697:706	arg1	slower					721:726	slower	721:726	slower	721:726	Test results of mass loss rate showed that the first 30 days of degradation was mainly starch degradation, and the degradation rate of esterified starch/PLA (ES/PLA) was slower than that of native starch/PLA (NS/PLA).
25192854	4	26	theme	starch/PLA	697:706	arg1	rate					678:681	the degradation rate	662:681	the degradation rate of esterified starch/PLA (ES/PLA)	662:715	Test results of mass loss rate showed that the first 30 days of degradation was mainly starch degradation, and the degradation rate of esterified starch/PLA (ES/PLA) was slower than that of native starch/PLA (NS/PLA).
25192854	10	27	theme	increasing	1861:1870	arg1	time					1856:1859	soil burial time increasing	1844:1870	soil burial time increasing	1844:1870	The tensile strength and bending strength of composites were decreased gradually with soil burial time increasing.
25192854	7	28	theme	crystalline	1483:1493	arg1	regions					1495:1501	its crystalline regions	1479:1501	its crystalline regions	1479:1501	The XRD diffraction peak intensity of PLA in composites showed an increased trend at first which was then followed by a decreased one along with the increasing time of soil burial degradation, indicating that the degradation of amorphous regions of PLA was earlier than its crystalline regions.
25192854	9	29	theme	degradation	1633:1643	arg1	time					1645:1648	the degradation time	1629:1648	the degradation time	1629:1648	If the degradation time was the same, T0, Ti and residual rate of thermal decomposition of NS/PLA were larger than those of ES/PLA.
25192854	9	29	theme	degradation	1633:1643	arg1	same					1658:1661	same	1658:1661	same	1658:1661	If the degradation time was the same, T0, Ti and residual rate of thermal decomposition of NS/PLA were larger than those of ES/PLA.
25192854	0	30	from	Effects	0:6	arg1	characteristics					66:80	the degradation characteristics	50:80	the degradation characteristics of starch/polylactic acid composites	50:117	Effects of dry method esterification of starch on the degradation characteristics of starch/polylactic acid composites.
25192854	8	31	from	intensity	1565:1573	arg1	ES/PLA					1585:1590	ES/PLA	1585:1590	ES/PLA	1585:1590	When the soil burial time was the same, the diffraction peak intensity of PLA in ES/PLA was stronger than that of NS/PLA.
25192854	0	32	theme	starch/polylactic	85:101	arg1	composites					108:117	starch/polylactic acid composites	85:117	starch/polylactic acid composites	85:117	Effects of dry method esterification of starch on the degradation characteristics of starch/polylactic acid composites.
25192854	5	33	from	ES/PLA	801:806	arg1	inside					827:832	the surface and inside	811:832	inside	827:832	Therefore, the damage degree of ES/PLA on the surface and inside was smaller than that of NS/PLA, and the infrared absorption peak intensities of C-O, C=O and C-H were stronger than that of NS/PLA.
25192854	5	33	from	ES/PLA	801:806	arg1	surface					815:821	the surface and inside	811:832	surface	815:821	Therefore, the damage degree of ES/PLA on the surface and inside was smaller than that of NS/PLA, and the infrared absorption peak intensities of C-O, C=O and C-H were stronger than that of NS/PLA.
25192854	1	34	theme	corn	148:151	arg1	starch					153:158	corn starch	148:158	corn starch	148:158	Maleic anhydride esterified corn starch was prepared by dry method.
25192854	3	35	theme	composites	460:469	arg1	characteristics					430:444	the degradation characteristics	414:444	the degradation characteristics of starch/PLA composites	414:469	The influence of the dry method esterification of starch on the degradation characteristics of starch/PLA composites was investigated by the natural aging degradation which was soil burial method.
25192854	0	36	theme	composites	108:117	arg1	characteristics					66:80	the degradation characteristics	50:80	the degradation characteristics of starch/polylactic acid composites	50:117	Effects of dry method esterification of starch on the degradation characteristics of starch/polylactic acid composites.
25192854	5	37	from	degree	791:796	arg1	inside					827:832	the surface and inside	811:832	inside	827:832	Therefore, the damage degree of ES/PLA on the surface and inside was smaller than that of NS/PLA, and the infrared absorption peak intensities of C-O, C=O and C-H were stronger than that of NS/PLA.
25192854	5	37	from	degree	791:796	arg1	surface					815:821	the surface and inside	811:832	surface	815:821	Therefore, the damage degree of ES/PLA on the surface and inside was smaller than that of NS/PLA, and the infrared absorption peak intensities of C-O, C=O and C-H were stronger than that of NS/PLA.
25192854	9	38	theme	residual	1675:1682	arg1	rate					1684:1687	residual rate	1675:1687	residual rate	1675:1687	If the degradation time was the same, T0, Ti and residual rate of thermal decomposition of NS/PLA were larger than those of ES/PLA.
25192854	6	39	theme	burial	1000:1005	arg1	degradation					1007:1017	soil burial degradation	995:1017	soil burial degradation	995:1017	With the increasing time of soil burial degradation, the damage degree of NS/PLA and ES/PLA on the exterior and interior were gradually increased, whereas the infrared absorption peak intensities of C-O, C=O and C-H were gradually decreased.
25192854	3	40	theme	method	379:384	arg1	esterification					386:399	the dry method esterification	371:399	the dry method esterification of starch	371:409	The influence of the dry method esterification of starch on the degradation characteristics of starch/PLA composites was investigated by the natural aging degradation which was soil burial method.
25192854	4	41	theme	loss	572:575	arg1	rate					577:580	mass loss rate	567:580	mass loss rate	567:580	Test results of mass loss rate showed that the first 30 days of degradation was mainly starch degradation, and the degradation rate of esterified starch/PLA (ES/PLA) was slower than that of native starch/PLA (NS/PLA).
25192854	6	42	from	exterior	1066:1073	arg1	degree					1031:1036	the damage degree	1020:1036	the damage degree of NS/PLA and ES/PLA on the exterior and interior	1020:1086	With the increasing time of soil burial degradation, the damage degree of NS/PLA and ES/PLA on the exterior and interior were gradually increased, whereas the infrared absorption peak intensities of C-O, C=O and C-H were gradually decreased.
25192854	0	43	theme	dry	11:13	arg1	esterification					22:35	dry method esterification	11:35	dry method esterification of starch	11:45	Effects of dry method esterification of starch on the degradation characteristics of starch/polylactic acid composites.
25192854	7	44	theme	regions	1447:1453	arg1	earlier					1466:1472	earlier	1466:1472	earlier	1466:1472	The XRD diffraction peak intensity of PLA in composites showed an increased trend at first which was then followed by a decreased one along with the increasing time of soil burial degradation, indicating that the degradation of amorphous regions of PLA was earlier than its crystalline regions.
25192854	7	44	theme	regions	1447:1453	arg1	degradation					1422:1432	the degradation	1418:1432	the degradation of amorphous regions of PLA	1418:1460	The XRD diffraction peak intensity of PLA in composites showed an increased trend at first which was then followed by a decreased one along with the increasing time of soil burial degradation, indicating that the degradation of amorphous regions of PLA was earlier than its crystalline regions.
25192854	3	45	theme	degradation	418:428	arg1	characteristics					430:444	the degradation characteristics	414:444	the degradation characteristics of starch/PLA composites	414:469	The influence of the dry method esterification of starch on the degradation characteristics of starch/PLA composites was investigated by the natural aging degradation which was soil burial method.
25192854	6	46	from	interior	1079:1086	arg1	degree					1031:1036	the damage degree	1020:1036	the damage degree of NS/PLA and ES/PLA on the exterior and interior	1020:1086	With the increasing time of soil burial degradation, the damage degree of NS/PLA and ES/PLA on the exterior and interior were gradually increased, whereas the infrared absorption peak intensities of C-O, C=O and C-H were gradually decreased.
25192854	0	47	theme	esterification	22:35	arg1	Effects					0:6	Effects	0:6	Effects of dry method esterification of starch on the degradation characteristics of starch/polylactic acid composites	0:117	Effects of dry method esterification of starch on the degradation characteristics of starch/polylactic acid composites.
25192854	6	48	theme	increasing	976:985	arg1	time					987:990	the increasing time	972:990	the increasing time of soil burial degradation	972:1017	With the increasing time of soil burial degradation, the damage degree of NS/PLA and ES/PLA on the exterior and interior were gradually increased, whereas the infrared absorption peak intensities of C-O, C=O and C-H were gradually decreased.
25192854	4	49	theme	Test	551:554	arg1	results					556:562	Test results	551:562	Test results of mass loss rate	551:580	Test results of mass loss rate showed that the first 30 days of degradation was mainly starch degradation, and the degradation rate of esterified starch/PLA (ES/PLA) was slower than that of native starch/PLA (NS/PLA).
25192854	5	50	theme	damage	784:789	arg1	smaller					838:844	smaller	838:844	smaller	838:844	Therefore, the damage degree of ES/PLA on the surface and inside was smaller than that of NS/PLA, and the infrared absorption peak intensities of C-O, C=O and C-H were stronger than that of NS/PLA.
25192854	5	50	theme	damage	784:789	arg1	degree					791:796	the damage degree	780:796	the damage degree of ES/PLA on the surface and inside	780:832	Therefore, the damage degree of ES/PLA on the surface and inside was smaller than that of NS/PLA, and the infrared absorption peak intensities of C-O, C=O and C-H were stronger than that of NS/PLA.
25192854	9	51	theme	decomposition	1700:1712	arg1	Ti					1668:1669	Ti	1668:1669	Ti	1668:1669	If the degradation time was the same, T0, Ti and residual rate of thermal decomposition of NS/PLA were larger than those of ES/PLA.
25192854	9	51	theme	decomposition	1700:1712	arg1	rate					1684:1687	residual rate	1675:1687	residual rate	1675:1687	If the degradation time was the same, T0, Ti and residual rate of thermal decomposition of NS/PLA were larger than those of ES/PLA.
25192854	9	51	theme	decomposition	1700:1712	arg1	T0					1664:1665	T0	1664:1665	T0	1664:1665	If the degradation time was the same, T0, Ti and residual rate of thermal decomposition of NS/PLA were larger than those of ES/PLA.
25192854	3	52	theme	aging	503:507	arg1	method					543:548	soil burial method	531:548	soil burial method	531:548	The influence of the dry method esterification of starch on the degradation characteristics of starch/PLA composites was investigated by the natural aging degradation which was soil burial method.
25192854	3	52	theme	aging	503:507	arg1	degradation					509:519	the natural aging degradation	491:519	the natural aging degradation which was soil burial method	491:548	The influence of the dry method esterification of starch on the degradation characteristics of starch/PLA composites was investigated by the natural aging degradation which was soil burial method.
25192854	9	53	theme	NS/PLA	1717:1722	arg1	Ti					1668:1669	Ti	1668:1669	Ti	1668:1669	If the degradation time was the same, T0, Ti and residual rate of thermal decomposition of NS/PLA were larger than those of ES/PLA.
25192854	9	53	theme	NS/PLA	1717:1722	arg1	rate					1684:1687	residual rate	1675:1687	residual rate	1675:1687	If the degradation time was the same, T0, Ti and residual rate of thermal decomposition of NS/PLA were larger than those of ES/PLA.
25192854	9	53	theme	NS/PLA	1717:1722	arg1	T0					1664:1665	T0	1664:1665	T0	1664:1665	If the degradation time was the same, T0, Ti and residual rate of thermal decomposition of NS/PLA were larger than those of ES/PLA.
25192854	7	54	theme	diffraction	1217:1227	arg1	intensity					1234:1242	The XRD diffraction peak intensity	1209:1242	The XRD diffraction peak intensity of PLA in composites	1209:1263	The XRD diffraction peak intensity of PLA in composites showed an increased trend at first which was then followed by a decreased one along with the increasing time of soil burial degradation, indicating that the degradation of amorphous regions of PLA was earlier than its crystalline regions.
25192854	8	55	theme	diffraction	1548:1558	arg1	stronger					1596:1603	stronger	1596:1603	stronger	1596:1603	When the soil burial time was the same, the diffraction peak intensity of PLA in ES/PLA was stronger than that of NS/PLA.
25192854	8	55	theme	diffraction	1548:1558	arg1	intensity					1565:1573	the diffraction peak intensity	1544:1573	the diffraction peak intensity of PLA in ES/PLA	1544:1590	When the soil burial time was the same, the diffraction peak intensity of PLA in ES/PLA was stronger than that of NS/PLA.
25192854	7	56	theme	degradation	1389:1399	arg1	time					1369:1372	the increasing time	1354:1372	the increasing time of soil burial degradation	1354:1399	The XRD diffraction peak intensity of PLA in composites showed an increased trend at first which was then followed by a decreased one along with the increasing time of soil burial degradation, indicating that the degradation of amorphous regions of PLA was earlier than its crystalline regions.
25192854	8	57	theme	soil	1513:1516	arg1	time					1525:1528	the soil burial time	1509:1528	the soil burial time	1509:1528	When the soil burial time was the same, the diffraction peak intensity of PLA in ES/PLA was stronger than that of NS/PLA.
25192854	8	57	theme	soil	1513:1516	arg1	same					1538:1541	same	1538:1541	same	1538:1541	When the soil burial time was the same, the diffraction peak intensity of PLA in ES/PLA was stronger than that of NS/PLA.
25192854	3	58	theme	soil	531:534	arg1	method					543:548	soil burial method	531:548	soil burial method	531:548	The influence of the dry method esterification of starch on the degradation characteristics of starch/PLA composites was investigated by the natural aging degradation which was soil burial method.
25192854	3	58	theme	soil	531:534	arg1	degradation					509:519	the natural aging degradation	491:519	the natural aging degradation which was soil burial method	491:548	The influence of the dry method esterification of starch on the degradation characteristics of starch/PLA composites was investigated by the natural aging degradation which was soil burial method.
25192854	7	59	theme	soil	1377:1380	arg1	degradation					1389:1399	soil burial degradation	1377:1399	soil burial degradation	1377:1399	The XRD diffraction peak intensity of PLA in composites showed an increased trend at first which was then followed by a decreased one along with the increasing time of soil burial degradation, indicating that the degradation of amorphous regions of PLA was earlier than its crystalline regions.
25192854	6	60	theme	damage	1024:1029	arg1	degree					1031:1036	the damage degree	1020:1036	the damage degree of NS/PLA and ES/PLA on the exterior and interior	1020:1086	With the increasing time of soil burial degradation, the damage degree of NS/PLA and ES/PLA on the exterior and interior were gradually increased, whereas the infrared absorption peak intensities of C-O, C=O and C-H were gradually decreased.
25192854	2	61	theme	maleic	305:310	arg1	anhydride					312:320	blending maleic anhydride esterified corn starch and PLA	296:351	blending maleic anhydride esterified corn starch and PLA	296:351	Esterified starch/polylactic acid (PLA) biodegradable composite was produced via melt extrusion method with blending maleic anhydride esterified corn starch and PLA.
25192854	8	62	theme	PLA	1578:1580	arg1	stronger					1596:1603	stronger	1596:1603	stronger	1596:1603	When the soil burial time was the same, the diffraction peak intensity of PLA in ES/PLA was stronger than that of NS/PLA.
25192854	8	62	theme	PLA	1578:1580	arg1	intensity					1565:1573	the diffraction peak intensity	1544:1573	the diffraction peak intensity of PLA in ES/PLA	1544:1590	When the soil burial time was the same, the diffraction peak intensity of PLA in ES/PLA was stronger than that of NS/PLA.
25192854	10	63	theme	bending	1783:1789	arg1	strength					1791:1798	bending strength	1783:1798	bending strength	1783:1798	The tensile strength and bending strength of composites were decreased gradually with soil burial time increasing.
25192854	1	64	theme	dry	176:178	arg1	method					180:185	dry method	176:185	dry method	176:185	Maleic anhydride esterified corn starch was prepared by dry method.
25192854	0	65	theme	starch	40:45	arg1	esterification					22:35	dry method esterification	11:35	dry method esterification of starch	11:45	Effects of dry method esterification of starch on the degradation characteristics of starch/polylactic acid composites.
25192854	6	66	dep	exterior	1066:1073	arg1	the					1062:1064	the	1062:1064	the	1062:1064	With the increasing time of soil burial degradation, the damage degree of NS/PLA and ES/PLA on the exterior and interior were gradually increased, whereas the infrared absorption peak intensities of C-O, C=O and C-H were gradually decreased.
25192854	2	67	theme	esterified	322:331	arg1	starch					338:343	esterified corn starch	322:343	esterified corn starch	322:343	Esterified starch/polylactic acid (PLA) biodegradable composite was produced via melt extrusion method with blending maleic anhydride esterified corn starch and PLA.
25192854	11	68	theme	ES/PLA	1923:1928	arg1	strength					1890:1897	tensile strength	1882:1897	tensile strength	1882:1897	Both the tensile strength and bending strength of ES/PLA were stronger than those of NS/PLA.
25192854	11	68	theme	ES/PLA	1923:1928	arg1	strength					1911:1918	bending strength	1903:1918	bending strength	1903:1918	Both the tensile strength and bending strength of ES/PLA were stronger than those of NS/PLA.
25192854	4	69	theme	starch	638:643	arg1	days					607:610	the first 30 days	594:610	the first 30 days of degradation	594:625	Test results of mass loss rate showed that the first 30 days of degradation was mainly starch degradation, and the degradation rate of esterified starch/PLA (ES/PLA) was slower than that of native starch/PLA (NS/PLA).
25192854	4	69	theme	starch	638:643	arg1	degradation					645:655	starch degradation	638:655	starch degradation	638:655	Test results of mass loss rate showed that the first 30 days of degradation was mainly starch degradation, and the degradation rate of esterified starch/PLA (ES/PLA) was slower than that of native starch/PLA (NS/PLA).
25192854	6	70	theme	C-O	1166:1168	arg1	intensities					1151:1161	the infrared absorption peak intensities	1122:1161	the infrared absorption peak intensities of C-O, C=O and C-H	1122:1181	With the increasing time of soil burial degradation, the damage degree of NS/PLA and ES/PLA on the exterior and interior were gradually increased, whereas the infrared absorption peak intensities of C-O, C=O and C-H were gradually decreased.
25192854	10	71	theme	soil	1844:1847	arg1	time					1856:1859	soil burial time increasing	1844:1870	soil burial time increasing	1844:1870	The tensile strength and bending strength of composites were decreased gradually with soil burial time increasing.
25192854	2	72	theme	biodegradable	228:240	arg1	composite					242:250	biodegradable composite	228:250	Esterified starch/polylactic acid (PLA) biodegradable composite	188:250	Esterified starch/polylactic acid (PLA) biodegradable composite was produced via melt extrusion method with blending maleic anhydride esterified corn starch and PLA.
25192854	5	73	from	inside	827:832	arg1	smaller					838:844	smaller	838:844	smaller	838:844	Therefore, the damage degree of ES/PLA on the surface and inside was smaller than that of NS/PLA, and the infrared absorption peak intensities of C-O, C=O and C-H were stronger than that of NS/PLA.
25192854	5	73	from	inside	827:832	arg1	degree					791:796	the damage degree	780:796	the damage degree of ES/PLA on the surface and inside	780:832	Therefore, the damage degree of ES/PLA on the surface and inside was smaller than that of NS/PLA, and the infrared absorption peak intensities of C-O, C=O and C-H were stronger than that of NS/PLA.
25192854	1	74	theme	Maleic	120:125	arg1	anhydride					127:135	Maleic anhydride	120:135	Maleic anhydride esterified corn starch	120:158	Maleic anhydride esterified corn starch was prepared by dry method.
25192854	2	75	dep	anhydride	312:320	arg1	PLA					349:351	PLA	349:351	PLA	349:351	Esterified starch/polylactic acid (PLA) biodegradable composite was produced via melt extrusion method with blending maleic anhydride esterified corn starch and PLA.
25192854	2	75	dep	anhydride	312:320	arg1	starch					338:343	esterified corn starch	322:343	esterified corn starch	322:343	Esterified starch/polylactic acid (PLA) biodegradable composite was produced via melt extrusion method with blending maleic anhydride esterified corn starch and PLA.
25192854	2	76	theme	extrusion	274:282	arg1	method					284:289	melt extrusion method	269:289	melt extrusion method with blending maleic anhydride esterified corn starch and PLA	269:351	Esterified starch/polylactic acid (PLA) biodegradable composite was produced via melt extrusion method with blending maleic anhydride esterified corn starch and PLA.
25192854	5	77	from	surface	815:821	arg1	smaller					838:844	smaller	838:844	smaller	838:844	Therefore, the damage degree of ES/PLA on the surface and inside was smaller than that of NS/PLA, and the infrared absorption peak intensities of C-O, C=O and C-H were stronger than that of NS/PLA.
25192854	5	77	from	surface	815:821	arg1	degree					791:796	the damage degree	780:796	the damage degree of ES/PLA on the surface and inside	780:832	Therefore, the damage degree of ES/PLA on the surface and inside was smaller than that of NS/PLA, and the infrared absorption peak intensities of C-O, C=O and C-H were stronger than that of NS/PLA.
25192854	5	78	theme	absorption	884:893	arg1	intensities					900:910	the infrared absorption peak intensities	871:910	the infrared absorption peak intensities of C-O, C=O and C-H	871:930	Therefore, the damage degree of ES/PLA on the surface and inside was smaller than that of NS/PLA, and the infrared absorption peak intensities of C-O, C=O and C-H were stronger than that of NS/PLA.
25192854	5	78	theme	absorption	884:893	arg1	stronger					937:944	stronger	937:944	stronger	937:944	Therefore, the damage degree of ES/PLA on the surface and inside was smaller than that of NS/PLA, and the infrared absorption peak intensities of C-O, C=O and C-H were stronger than that of NS/PLA.
25192854	6	79	theme	absorption	1135:1144	arg1	intensities					1151:1161	the infrared absorption peak intensities	1122:1161	the infrared absorption peak intensities of C-O, C=O and C-H	1122:1181	With the increasing time of soil burial degradation, the damage degree of NS/PLA and ES/PLA on the exterior and interior were gradually increased, whereas the infrared absorption peak intensities of C-O, C=O and C-H were gradually decreased.
25192854	0	80	theme	degradation	54:64	arg1	characteristics					66:80	the degradation characteristics	50:80	the degradation characteristics of starch/polylactic acid composites	50:117	Effects of dry method esterification of starch on the degradation characteristics of starch/polylactic acid composites.
25192854	4	81	theme	esterified	686:695	arg1	ES/PLA					709:714	ES/PLA	709:714	ES/PLA	709:714	Test results of mass loss rate showed that the first 30 days of degradation was mainly starch degradation, and the degradation rate of esterified starch/PLA (ES/PLA) was slower than that of native starch/PLA (NS/PLA).
25192854	4	81	theme	esterified	686:695	arg1	starch/PLA					697:706	esterified starch/PLA	686:706	esterified starch/PLA (ES/PLA)	686:715	Test results of mass loss rate showed that the first 30 days of degradation was mainly starch degradation, and the degradation rate of esterified starch/PLA (ES/PLA) was slower than that of native starch/PLA (NS/PLA).
25192854	11	82	dep	strength	1890:1897	arg1	the					1878:1880	the	1878:1880	the	1878:1880	Both the tensile strength and bending strength of ES/PLA were stronger than those of NS/PLA.
25192854	0	83	theme	acid	103:106	arg1	composites					108:117	starch/polylactic acid composites	85:117	starch/polylactic acid composites	85:117	Effects of dry method esterification of starch on the degradation characteristics of starch/polylactic acid composites.
25192854	5	84	theme	C=O	920:922	arg1	intensities					900:910	the infrared absorption peak intensities	871:910	the infrared absorption peak intensities of C-O, C=O and C-H	871:930	Therefore, the damage degree of ES/PLA on the surface and inside was smaller than that of NS/PLA, and the infrared absorption peak intensities of C-O, C=O and C-H were stronger than that of NS/PLA.
25192854	5	84	theme	C=O	920:922	arg1	stronger					937:944	stronger	937:944	stronger	937:944	Therefore, the damage degree of ES/PLA on the surface and inside was smaller than that of NS/PLA, and the infrared absorption peak intensities of C-O, C=O and C-H were stronger than that of NS/PLA.
25192854	7	85	theme	PLA	1458:1460	arg1	regions					1447:1453	amorphous regions	1437:1453	amorphous regions of PLA	1437:1460	The XRD diffraction peak intensity of PLA in composites showed an increased trend at first which was then followed by a decreased one along with the increasing time of soil burial degradation, indicating that the degradation of amorphous regions of PLA was earlier than its crystalline regions.
25192854	3	86	theme	dry	375:377	arg1	esterification					386:399	the dry method esterification	371:399	the dry method esterification of starch	371:409	The influence of the dry method esterification of starch on the degradation characteristics of starch/PLA composites was investigated by the natural aging degradation which was soil burial method.
25192854	5	87	theme	C-H	928:930	arg1	intensities					900:910	the infrared absorption peak intensities	871:910	the infrared absorption peak intensities of C-O, C=O and C-H	871:930	Therefore, the damage degree of ES/PLA on the surface and inside was smaller than that of NS/PLA, and the infrared absorption peak intensities of C-O, C=O and C-H were stronger than that of NS/PLA.
25192854	5	87	theme	C-H	928:930	arg1	stronger					937:944	stronger	937:944	stronger	937:944	Therefore, the damage degree of ES/PLA on the surface and inside was smaller than that of NS/PLA, and the infrared absorption peak intensities of C-O, C=O and C-H were stronger than that of NS/PLA.
25192854	6	88	theme	degradation	1007:1017	arg1	time					987:990	the increasing time	972:990	the increasing time of soil burial degradation	972:1017	With the increasing time of soil burial degradation, the damage degree of NS/PLA and ES/PLA on the exterior and interior were gradually increased, whereas the infrared absorption peak intensities of C-O, C=O and C-H were gradually decreased.
25192854	3	89	theme	starch	404:409	arg1	esterification					386:399	the dry method esterification	371:399	the dry method esterification of starch	371:409	The influence of the dry method esterification of starch on the degradation characteristics of starch/PLA composites was investigated by the natural aging degradation which was soil burial method.
25192854	4	90	theme	rate	577:580	arg1	results					556:562	Test results	551:562	Test results of mass loss rate	551:580	Test results of mass loss rate showed that the first 30 days of degradation was mainly starch degradation, and the degradation rate of esterified starch/PLA (ES/PLA) was slower than that of native starch/PLA (NS/PLA).
25192854	2	91	theme	starch/polylactic	199:215	arg1	acid					217:220	Esterified starch/polylactic acid	188:220	Esterified starch/polylactic acid (PLA) biodegradable composite	188:250	Esterified starch/polylactic acid (PLA) biodegradable composite was produced via melt extrusion method with blending maleic anhydride esterified corn starch and PLA.
25192854	2	91	theme	starch/polylactic	199:215	arg1	PLA					223:225	PLA	223:225	PLA	223:225	Esterified starch/polylactic acid (PLA) biodegradable composite was produced via melt extrusion method with blending maleic anhydride esterified corn starch and PLA.
25192854	3	92	theme	starch/PLA	449:458	arg1	composites					460:469	starch/PLA composites	449:469	starch/PLA composites	449:469	The influence of the dry method esterification of starch on the degradation characteristics of starch/PLA composites was investigated by the natural aging degradation which was soil burial method.
25192854	0	93	theme	method	15:20	arg1	esterification					22:35	dry method esterification	11:35	dry method esterification of starch	11:45	Effects of dry method esterification of starch on the degradation characteristics of starch/polylactic acid composites.
25192854	5	94	theme	C-O	915:917	arg1	intensities					900:910	the infrared absorption peak intensities	871:910	the infrared absorption peak intensities of C-O, C=O and C-H	871:930	Therefore, the damage degree of ES/PLA on the surface and inside was smaller than that of NS/PLA, and the infrared absorption peak intensities of C-O, C=O and C-H were stronger than that of NS/PLA.
25192854	5	94	theme	C-O	915:917	arg1	stronger					937:944	stronger	937:944	stronger	937:944	Therefore, the damage degree of ES/PLA on the surface and inside was smaller than that of NS/PLA, and the infrared absorption peak intensities of C-O, C=O and C-H were stronger than that of NS/PLA.
25192854	7	95	from	first	1294:1298	arg1	trend					1285:1289	an increased trend	1272:1289	an increased trend at first which was then followed by a decreased one	1272:1341	The XRD diffraction peak intensity of PLA in composites showed an increased trend at first which was then followed by a decreased one along with the increasing time of soil burial degradation, indicating that the degradation of amorphous regions of PLA was earlier than its crystalline regions.
25192854	6	96	theme	soil	995:998	arg1	degradation					1007:1017	soil burial degradation	995:1017	soil burial degradation	995:1017	With the increasing time of soil burial degradation, the damage degree of NS/PLA and ES/PLA on the exterior and interior were gradually increased, whereas the infrared absorption peak intensities of C-O, C=O and C-H were gradually decreased.
25192854	3	97	theme	esterification	386:399	arg1	influence					358:366	The influence	354:366	The influence of the dry method esterification of starch on the degradation characteristics of starch/PLA composites	354:469	The influence of the dry method esterification of starch on the degradation characteristics of starch/PLA composites was investigated by the natural aging degradation which was soil burial method.
25192854	4	98	theme	degradation	615:625	arg1	days					607:610	the first 30 days	594:610	the first 30 days of degradation	594:625	Test results of mass loss rate showed that the first 30 days of degradation was mainly starch degradation, and the degradation rate of esterified starch/PLA (ES/PLA) was slower than that of native starch/PLA (NS/PLA).
25192854	4	98	theme	degradation	615:625	arg1	degradation					645:655	starch degradation	638:655	starch degradation	638:655	Test results of mass loss rate showed that the first 30 days of degradation was mainly starch degradation, and the degradation rate of esterified starch/PLA (ES/PLA) was slower than that of native starch/PLA (NS/PLA).
25192854	2	99	theme	Esterified	188:197	arg1	acid					217:220	Esterified starch/polylactic acid	188:220	Esterified starch/polylactic acid (PLA) biodegradable composite	188:250	Esterified starch/polylactic acid (PLA) biodegradable composite was produced via melt extrusion method with blending maleic anhydride esterified corn starch and PLA.
25192854	2	99	theme	Esterified	188:197	arg1	PLA					223:225	PLA	223:225	PLA	223:225	Esterified starch/polylactic acid (PLA) biodegradable composite was produced via melt extrusion method with blending maleic anhydride esterified corn starch and PLA.
25192854	4	100	theme	native	741:746	arg1	NS/PLA					760:765	NS/PLA	760:765	NS/PLA	760:765	Test results of mass loss rate showed that the first 30 days of degradation was mainly starch degradation, and the degradation rate of esterified starch/PLA (ES/PLA) was slower than that of native starch/PLA (NS/PLA).
25192854	4	100	theme	native	741:746	arg1	starch/PLA					748:757	native starch/PLA	741:757	native starch/PLA (NS/PLA)	741:766	Test results of mass loss rate showed that the first 30 days of degradation was mainly starch degradation, and the degradation rate of esterified starch/PLA (ES/PLA) was slower than that of native starch/PLA (NS/PLA).
25192854	3	101	theme	natural	495:501	arg1	method					543:548	soil burial method	531:548	soil burial method	531:548	The influence of the dry method esterification of starch on the degradation characteristics of starch/PLA composites was investigated by the natural aging degradation which was soil burial method.
25192854	3	101	theme	natural	495:501	arg1	degradation					509:519	the natural aging degradation	491:519	the natural aging degradation which was soil burial method	491:548	The influence of the dry method esterification of starch on the degradation characteristics of starch/PLA composites was investigated by the natural aging degradation which was soil burial method.
25192854	7	102	theme	XRD	1213:1215	arg1	intensity					1234:1242	The XRD diffraction peak intensity	1209:1242	The XRD diffraction peak intensity of PLA in composites	1209:1263	The XRD diffraction peak intensity of PLA in composites showed an increased trend at first which was then followed by a decreased one along with the increasing time of soil burial degradation, indicating that the degradation of amorphous regions of PLA was earlier than its crystalline regions.
25192854	6	103	from	ES/PLA	1052:1057	arg1	interior					1079:1086	interior	1079:1086	interior	1079:1086	With the increasing time of soil burial degradation, the damage degree of NS/PLA and ES/PLA on the exterior and interior were gradually increased, whereas the infrared absorption peak intensities of C-O, C=O and C-H were gradually decreased.
25192854	6	103	from	ES/PLA	1052:1057	arg1	exterior					1066:1073	exterior	1066:1073	exterior	1066:1073	With the increasing time of soil burial degradation, the damage degree of NS/PLA and ES/PLA on the exterior and interior were gradually increased, whereas the infrared absorption peak intensities of C-O, C=O and C-H were gradually decreased.
25192854	7	104	theme	amorphous	1437:1445	arg1	regions					1447:1453	amorphous regions	1437:1453	amorphous regions of PLA	1437:1460	The XRD diffraction peak intensity of PLA in composites showed an increased trend at first which was then followed by a decreased one along with the increasing time of soil burial degradation, indicating that the degradation of amorphous regions of PLA was earlier than its crystalline regions.
25192854	9	105	theme	thermal	1692:1698	arg1	decomposition					1700:1712	thermal decomposition	1692:1712	thermal decomposition	1692:1712	If the degradation time was the same, T0, Ti and residual rate of thermal decomposition of NS/PLA were larger than those of ES/PLA.
25192854	4	106	theme	mass	567:570	arg1	rate					577:580	mass loss rate	567:580	mass loss rate	567:580	Test results of mass loss rate showed that the first 30 days of degradation was mainly starch degradation, and the degradation rate of esterified starch/PLA (ES/PLA) was slower than that of native starch/PLA (NS/PLA).
25192854	7	107	from	intensity	1234:1242	arg1	composites					1254:1263	composites	1254:1263	composites	1254:1263	The XRD diffraction peak intensity of PLA in composites showed an increased trend at first which was then followed by a decreased one along with the increasing time of soil burial degradation, indicating that the degradation of amorphous regions of PLA was earlier than its crystalline regions.
25192854	6	108	theme	ES/PLA	1052:1057	arg1	degree					1031:1036	the damage degree	1020:1036	the damage degree of NS/PLA and ES/PLA on the exterior and interior	1020:1086	With the increasing time of soil burial degradation, the damage degree of NS/PLA and ES/PLA on the exterior and interior were gradually increased, whereas the infrared absorption peak intensities of C-O, C=O and C-H were gradually decreased.
25192854	2	109	dep	acid	217:220	arg1	composite					242:250	biodegradable composite	228:250	Esterified starch/polylactic acid (PLA) biodegradable composite	188:250	Esterified starch/polylactic acid (PLA) biodegradable composite was produced via melt extrusion method with blending maleic anhydride esterified corn starch and PLA.
25192854	6	110	from	degree	1031:1036	arg1	interior					1079:1086	interior	1079:1086	interior	1079:1086	With the increasing time of soil burial degradation, the damage degree of NS/PLA and ES/PLA on the exterior and interior were gradually increased, whereas the infrared absorption peak intensities of C-O, C=O and C-H were gradually decreased.
25192854	6	110	from	degree	1031:1036	arg1	exterior					1066:1073	exterior	1066:1073	exterior	1066:1073	With the increasing time of soil burial degradation, the damage degree of NS/PLA and ES/PLA on the exterior and interior were gradually increased, whereas the infrared absorption peak intensities of C-O, C=O and C-H were gradually decreased.
25192854	8	111	theme	peak	1560:1563	arg1	stronger					1596:1603	stronger	1596:1603	stronger	1596:1603	When the soil burial time was the same, the diffraction peak intensity of PLA in ES/PLA was stronger than that of NS/PLA.
25192854	8	111	theme	peak	1560:1563	arg1	intensity					1565:1573	the diffraction peak intensity	1544:1573	the diffraction peak intensity of PLA in ES/PLA	1544:1590	When the soil burial time was the same, the diffraction peak intensity of PLA in ES/PLA was stronger than that of NS/PLA.
25192854	6	112	theme	NS/PLA	1041:1046	arg1	degree					1031:1036	the damage degree	1020:1036	the damage degree of NS/PLA and ES/PLA on the exterior and interior	1020:1086	With the increasing time of soil burial degradation, the damage degree of NS/PLA and ES/PLA on the exterior and interior were gradually increased, whereas the infrared absorption peak intensities of C-O, C=O and C-H were gradually decreased.
25192854	8	113	theme	burial	1518:1523	arg1	time					1525:1528	the soil burial time	1509:1528	the soil burial time	1509:1528	When the soil burial time was the same, the diffraction peak intensity of PLA in ES/PLA was stronger than that of NS/PLA.
25192854	8	113	theme	burial	1518:1523	arg1	same					1538:1541	same	1538:1541	same	1538:1541	When the soil burial time was the same, the diffraction peak intensity of PLA in ES/PLA was stronger than that of NS/PLA.
25192854	10	114	theme	tensile	1762:1768	arg1	strength					1770:1777	tensile strength	1762:1777	tensile strength	1762:1777	The tensile strength and bending strength of composites were decreased gradually with soil burial time increasing.
25192854	6	115	from	NS/PLA	1041:1046	arg1	interior					1079:1086	interior	1079:1086	interior	1079:1086	With the increasing time of soil burial degradation, the damage degree of NS/PLA and ES/PLA on the exterior and interior were gradually increased, whereas the infrared absorption peak intensities of C-O, C=O and C-H were gradually decreased.
25192854	6	115	from	NS/PLA	1041:1046	arg1	exterior					1066:1073	exterior	1066:1073	exterior	1066:1073	With the increasing time of soil burial degradation, the damage degree of NS/PLA and ES/PLA on the exterior and interior were gradually increased, whereas the infrared absorption peak intensities of C-O, C=O and C-H were gradually decreased.
28707621	4	0	theme	Newman-Keuls	956:967	arg1	test					978:981	Newman-Keuls post hoc test	956:981	Newman-Keuls post hoc test	956:981	In addition, we subjected the obtained results to statistical analysis (factor analysis, t-test, and Newman-Keuls post hoc test).
28707621	3	1	theme	disaccharide	814:825	arg1	analysis					827:834	the detailed disaccharide analysis	801:834	the detailed disaccharide analysis of new LMWH drugs	801:852	For this purpose, we used strong anion-exchange (SAX) chromatography with spectrophotometric detection because this method is more helpful, easier, and faster than other separation techniques for the detailed disaccharide analysis of new LMWH drugs.
28707621	1	2	theme	analytical	193:202	arg1	characterization					122:137	detailed structural characterization	102:137	detailed structural characterization of low-molecular-weight heparin (LMWH) products	102:185	Currently, detailed structural characterization of low-molecular-weight heparin (LMWH) products is an analytical subject of great interest.
28707621	1	2	theme	analytical	193:202	arg1	subject					204:210	an analytical subject	190:210	an analytical subject of great interest	190:228	Currently, detailed structural characterization of low-molecular-weight heparin (LMWH) products is an analytical subject of great interest.
28707621	4	3	theme	factor	927:932	arg1	analysis					934:941	factor analysis	927:941	factor analysis	927:941	In addition, we subjected the obtained results to statistical analysis (factor analysis, t-test, and Newman-Keuls post hoc test).
28707621	2	4	theme	quantitative	506:517	arg1	composition					519:529	the qualitative and quantitative composition	486:529	the qualitative and quantitative composition of commercially available drugs (enoxaparin, nadroparin, and dalteparin)	486:602	In this work, we carried out a comprehensive structural analysis of LMWHs and applied a modified pharmacopeial method, as well as methods developed by other researchers, to the analysis of novel biosimilar LMWH products; and, for the first time, compared the qualitative and quantitative composition of commercially available drugs (enoxaparin, nadroparin, and dalteparin).
28707621	3	5	theme	detailed	805:812	arg1	analysis					827:834	the detailed disaccharide analysis	801:834	the detailed disaccharide analysis of new LMWH drugs	801:852	For this purpose, we used strong anion-exchange (SAX) chromatography with spectrophotometric detection because this method is more helpful, easier, and faster than other separation techniques for the detailed disaccharide analysis of new LMWH drugs.
28707621	2	6	dep	drugs	557:561	arg1	dalteparin					592:601	dalteparin	592:601	dalteparin	592:601	In this work, we carried out a comprehensive structural analysis of LMWHs and applied a modified pharmacopeial method, as well as methods developed by other researchers, to the analysis of novel biosimilar LMWH products; and, for the first time, compared the qualitative and quantitative composition of commercially available drugs (enoxaparin, nadroparin, and dalteparin).
28707621	2	6	dep	drugs	557:561	arg1	enoxaparin					564:573	enoxaparin	564:573	enoxaparin	564:573	In this work, we carried out a comprehensive structural analysis of LMWHs and applied a modified pharmacopeial method, as well as methods developed by other researchers, to the analysis of novel biosimilar LMWH products; and, for the first time, compared the qualitative and quantitative composition of commercially available drugs (enoxaparin, nadroparin, and dalteparin).
28707621	2	6	dep	drugs	557:561	arg1	drugs					557:561	commercially available drugs	534:561	commercially available drugs (enoxaparin, nadroparin, and dalteparin)	534:602	In this work, we carried out a comprehensive structural analysis of LMWHs and applied a modified pharmacopeial method, as well as methods developed by other researchers, to the analysis of novel biosimilar LMWH products; and, for the first time, compared the qualitative and quantitative composition of commercially available drugs (enoxaparin, nadroparin, and dalteparin).
28707621	2	6	dep	drugs	557:561	arg1	nadroparin					576:585	nadroparin	576:585	nadroparin	576:585	In this work, we carried out a comprehensive structural analysis of LMWHs and applied a modified pharmacopeial method, as well as methods developed by other researchers, to the analysis of novel biosimilar LMWH products; and, for the first time, compared the qualitative and quantitative composition of commercially available drugs (enoxaparin, nadroparin, and dalteparin).
28707621	2	7	theme	LMWHs	299:303	arg1	analysis					287:294	a comprehensive structural analysis	260:294	a comprehensive structural analysis of LMWHs	260:303	In this work, we carried out a comprehensive structural analysis of LMWHs and applied a modified pharmacopeial method, as well as methods developed by other researchers, to the analysis of novel biosimilar LMWH products; and, for the first time, compared the qualitative and quantitative composition of commercially available drugs (enoxaparin, nadroparin, and dalteparin).
28707621	2	8	theme	qualitative	490:500	arg1	composition					519:529	the qualitative and quantitative composition	486:529	the qualitative and quantitative composition of commercially available drugs (enoxaparin, nadroparin, and dalteparin)	486:602	In this work, we carried out a comprehensive structural analysis of LMWHs and applied a modified pharmacopeial method, as well as methods developed by other researchers, to the analysis of novel biosimilar LMWH products; and, for the first time, compared the qualitative and quantitative composition of commercially available drugs (enoxaparin, nadroparin, and dalteparin).
28707621	2	9	theme	first	465:469	arg1	time					471:474	the first time	461:474	the first time	461:474	In this work, we carried out a comprehensive structural analysis of LMWHs and applied a modified pharmacopeial method, as well as methods developed by other researchers, to the analysis of novel biosimilar LMWH products; and, for the first time, compared the qualitative and quantitative composition of commercially available drugs (enoxaparin, nadroparin, and dalteparin).
28707621	1	10	theme	detailed	102:109	arg1	characterization					122:137	detailed structural characterization	102:137	detailed structural characterization of low-molecular-weight heparin (LMWH) products	102:185	Currently, detailed structural characterization of low-molecular-weight heparin (LMWH) products is an analytical subject of great interest.
28707621	1	10	theme	detailed	102:109	arg1	subject					204:210	an analytical subject	190:210	an analytical subject of great interest	190:228	Currently, detailed structural characterization of low-molecular-weight heparin (LMWH) products is an analytical subject of great interest.
28707621	1	11	theme	structural	111:120	arg1	characterization					122:137	detailed structural characterization	102:137	detailed structural characterization of low-molecular-weight heparin (LMWH) products	102:185	Currently, detailed structural characterization of low-molecular-weight heparin (LMWH) products is an analytical subject of great interest.
28707621	1	11	theme	structural	111:120	arg1	subject					204:210	an analytical subject	190:210	an analytical subject of great interest	190:228	Currently, detailed structural characterization of low-molecular-weight heparin (LMWH) products is an analytical subject of great interest.
28707621	1	12	theme	great	215:219	arg1	interest					221:228	great interest	215:228	great interest	215:228	Currently, detailed structural characterization of low-molecular-weight heparin (LMWH) products is an analytical subject of great interest.
28707621	2	13	theme	pharmacopeial	328:340	arg1	method					342:347	a modified pharmacopeial method	317:347	a modified pharmacopeial method	317:347	In this work, we carried out a comprehensive structural analysis of LMWHs and applied a modified pharmacopeial method, as well as methods developed by other researchers, to the analysis of novel biosimilar LMWH products; and, for the first time, compared the qualitative and quantitative composition of commercially available drugs (enoxaparin, nadroparin, and dalteparin).
28707621	1	14	theme	interest	221:228	arg1	characterization					122:137	detailed structural characterization	102:137	detailed structural characterization of low-molecular-weight heparin (LMWH) products	102:185	Currently, detailed structural characterization of low-molecular-weight heparin (LMWH) products is an analytical subject of great interest.
28707621	1	14	theme	interest	221:228	arg1	subject					204:210	an analytical subject	190:210	an analytical subject of great interest	190:228	Currently, detailed structural characterization of low-molecular-weight heparin (LMWH) products is an analytical subject of great interest.
28707621	2	15	theme	products	442:449	arg1	analysis					408:415	the analysis	404:415	the analysis of novel biosimilar LMWH products	404:449	In this work, we carried out a comprehensive structural analysis of LMWHs and applied a modified pharmacopeial method, as well as methods developed by other researchers, to the analysis of novel biosimilar LMWH products; and, for the first time, compared the qualitative and quantitative composition of commercially available drugs (enoxaparin, nadroparin, and dalteparin).
28707621	3	16	theme	other	769:773	arg1	techniques					786:795	other separation techniques	769:795	other separation techniques for the detailed disaccharide analysis of new LMWH drugs	769:852	For this purpose, we used strong anion-exchange (SAX) chromatography with spectrophotometric detection because this method is more helpful, easier, and faster than other separation techniques for the detailed disaccharide analysis of new LMWH drugs.
28707621	2	17	theme	drugs	557:561	arg1	composition					519:529	the qualitative and quantitative composition	486:529	the qualitative and quantitative composition of commercially available drugs (enoxaparin, nadroparin, and dalteparin)	486:602	In this work, we carried out a comprehensive structural analysis of LMWHs and applied a modified pharmacopeial method, as well as methods developed by other researchers, to the analysis of novel biosimilar LMWH products; and, for the first time, compared the qualitative and quantitative composition of commercially available drugs (enoxaparin, nadroparin, and dalteparin).
28707621	2	18	theme	modified	319:326	arg1	method					342:347	a modified pharmacopeial method	317:347	a modified pharmacopeial method	317:347	In this work, we carried out a comprehensive structural analysis of LMWHs and applied a modified pharmacopeial method, as well as methods developed by other researchers, to the analysis of novel biosimilar LMWH products; and, for the first time, compared the qualitative and quantitative composition of commercially available drugs (enoxaparin, nadroparin, and dalteparin).
28707621	2	19	theme	biosimilar	426:435	arg1	products					442:449	novel biosimilar LMWH products	420:449	novel biosimilar LMWH products	420:449	In this work, we carried out a comprehensive structural analysis of LMWHs and applied a modified pharmacopeial method, as well as methods developed by other researchers, to the analysis of novel biosimilar LMWH products; and, for the first time, compared the qualitative and quantitative composition of commercially available drugs (enoxaparin, nadroparin, and dalteparin).
28707621	2	20	theme	LMWH	437:440	arg1	products					442:449	novel biosimilar LMWH products	420:449	novel biosimilar LMWH products	420:449	In this work, we carried out a comprehensive structural analysis of LMWHs and applied a modified pharmacopeial method, as well as methods developed by other researchers, to the analysis of novel biosimilar LMWH products; and, for the first time, compared the qualitative and quantitative composition of commercially available drugs (enoxaparin, nadroparin, and dalteparin).
28707621	2	21	theme	available	547:555	arg1	dalteparin					592:601	dalteparin	592:601	dalteparin	592:601	In this work, we carried out a comprehensive structural analysis of LMWHs and applied a modified pharmacopeial method, as well as methods developed by other researchers, to the analysis of novel biosimilar LMWH products; and, for the first time, compared the qualitative and quantitative composition of commercially available drugs (enoxaparin, nadroparin, and dalteparin).
28707621	2	21	theme	available	547:555	arg1	enoxaparin					564:573	enoxaparin	564:573	enoxaparin	564:573	In this work, we carried out a comprehensive structural analysis of LMWHs and applied a modified pharmacopeial method, as well as methods developed by other researchers, to the analysis of novel biosimilar LMWH products; and, for the first time, compared the qualitative and quantitative composition of commercially available drugs (enoxaparin, nadroparin, and dalteparin).
28707621	2	21	theme	available	547:555	arg1	drugs					557:561	commercially available drugs	534:561	commercially available drugs (enoxaparin, nadroparin, and dalteparin)	534:602	In this work, we carried out a comprehensive structural analysis of LMWHs and applied a modified pharmacopeial method, as well as methods developed by other researchers, to the analysis of novel biosimilar LMWH products; and, for the first time, compared the qualitative and quantitative composition of commercially available drugs (enoxaparin, nadroparin, and dalteparin).
28707621	2	21	theme	available	547:555	arg1	nadroparin					576:585	nadroparin	576:585	nadroparin	576:585	In this work, we carried out a comprehensive structural analysis of LMWHs and applied a modified pharmacopeial method, as well as methods developed by other researchers, to the analysis of novel biosimilar LMWH products; and, for the first time, compared the qualitative and quantitative composition of commercially available drugs (enoxaparin, nadroparin, and dalteparin).
28707621	1	22	theme	low-molecular-weight	142:161	arg1	LMWH					172:175	LMWH	172:175	LMWH	172:175	Currently, detailed structural characterization of low-molecular-weight heparin (LMWH) products is an analytical subject of great interest.
28707621	1	22	theme	low-molecular-weight	142:161	arg1	heparin					163:169	low-molecular-weight heparin	142:169	low-molecular-weight heparin (LMWH) products	142:185	Currently, detailed structural characterization of low-molecular-weight heparin (LMWH) products is an analytical subject of great interest.
28707621	2	23	theme	novel	420:424	arg1	products					442:449	novel biosimilar LMWH products	420:449	novel biosimilar LMWH products	420:449	In this work, we carried out a comprehensive structural analysis of LMWHs and applied a modified pharmacopeial method, as well as methods developed by other researchers, to the analysis of novel biosimilar LMWH products; and, for the first time, compared the qualitative and quantitative composition of commercially available drugs (enoxaparin, nadroparin, and dalteparin).
28707621	0	24	theme	Strong	53:58	arg1	Chromatography					75:88	Strong Anion-Exchange Chromatography	53:88	Strong Anion-Exchange Chromatography	53:88	Characterization of Low-Molecular-Weight Heparins by Strong Anion-Exchange Chromatography.
28707621	3	25	used	used	626:629	arg2	we					623:624	we	623:624	we	623:624	For this purpose, we used strong anion-exchange (SAX) chromatography with spectrophotometric detection because this method is more helpful, easier, and faster than other separation techniques for the detailed disaccharide analysis of new LMWH drugs.
28707621	3	26	theme	strong	631:636	arg1	chromatography					659:672	strong anion-exchange (SAX) chromatography	631:672	strong anion-exchange (SAX) chromatography with spectrophotometric detection	631:706	For this purpose, we used strong anion-exchange (SAX) chromatography with spectrophotometric detection because this method is more helpful, easier, and faster than other separation techniques for the detailed disaccharide analysis of new LMWH drugs.
28707621	4	27	theme	post	969:972	arg1	test					978:981	Newman-Keuls post hoc test	956:981	Newman-Keuls post hoc test	956:981	In addition, we subjected the obtained results to statistical analysis (factor analysis, t-test, and Newman-Keuls post hoc test).
28707621	4	28	theme	statistical	905:915	arg1	analysis					917:924	statistical analysis	905:924	statistical analysis (factor analysis, t-test, and Newman-Keuls post hoc test)	905:982	In addition, we subjected the obtained results to statistical analysis (factor analysis, t-test, and Newman-Keuls post hoc test).
28707621	0	29	theme	Heparins	41:48	arg1	Characterization					0:15	Characterization	0:15	Characterization of Low-Molecular-Weight Heparins by Strong Anion-Exchange Chromatography.	0:89	Characterization of Low-Molecular-Weight Heparins by Strong Anion-Exchange Chromatography.
28707621	2	30	dep	carried	248:254	arg1	compared					477:484	compared	477:484	compared the qualitative and quantitative composition of commercially available drugs (enoxaparin, nadroparin, and dalteparin)	477:602	In this work, we carried out a comprehensive structural analysis of LMWHs and applied a modified pharmacopeial method, as well as methods developed by other researchers, to the analysis of novel biosimilar LMWH products; and, for the first time, compared the qualitative and quantitative composition of commercially available drugs (enoxaparin, nadroparin, and dalteparin).
28707621	4	31	dep	analysis	917:924	arg1	test					978:981	Newman-Keuls post hoc test	956:981	Newman-Keuls post hoc test	956:981	In addition, we subjected the obtained results to statistical analysis (factor analysis, t-test, and Newman-Keuls post hoc test).
28707621	4	31	dep	analysis	917:924	arg1	analysis					934:941	factor analysis	927:941	factor analysis	927:941	In addition, we subjected the obtained results to statistical analysis (factor analysis, t-test, and Newman-Keuls post hoc test).
28707621	4	31	dep	analysis	917:924	arg1	t-test					944:949	t-test	944:949	t-test	944:949	In addition, we subjected the obtained results to statistical analysis (factor analysis, t-test, and Newman-Keuls post hoc test).
28707621	1	32	theme	heparin	163:169	arg1	products					178:185	low-molecular-weight heparin (LMWH) products	142:185	low-molecular-weight heparin (LMWH) products	142:185	Currently, detailed structural characterization of low-molecular-weight heparin (LMWH) products is an analytical subject of great interest.
28707621	0	33	theme	Anion-Exchange	60:73	arg1	Chromatography					75:88	Strong Anion-Exchange Chromatography	53:88	Strong Anion-Exchange Chromatography	53:88	Characterization of Low-Molecular-Weight Heparins by Strong Anion-Exchange Chromatography.
28707621	3	34	theme	SAX	654:656	arg1	chromatography					659:672	strong anion-exchange (SAX) chromatography	631:672	strong anion-exchange (SAX) chromatography with spectrophotometric detection	631:706	For this purpose, we used strong anion-exchange (SAX) chromatography with spectrophotometric detection because this method is more helpful, easier, and faster than other separation techniques for the detailed disaccharide analysis of new LMWH drugs.
28707621	3	35	theme	LMWH	843:846	arg1	drugs					848:852	new LMWH drugs	839:852	new LMWH drugs	839:852	For this purpose, we used strong anion-exchange (SAX) chromatography with spectrophotometric detection because this method is more helpful, easier, and faster than other separation techniques for the detailed disaccharide analysis of new LMWH drugs.
28707621	4	36	theme	obtained	885:892	arg1	results					894:900	the obtained results	881:900	the obtained results	881:900	In addition, we subjected the obtained results to statistical analysis (factor analysis, t-test, and Newman-Keuls post hoc test).
28707621	1	37	theme	products	178:185	arg1	characterization					122:137	detailed structural characterization	102:137	detailed structural characterization of low-molecular-weight heparin (LMWH) products	102:185	Currently, detailed structural characterization of low-molecular-weight heparin (LMWH) products is an analytical subject of great interest.
28707621	1	37	theme	products	178:185	arg1	subject					204:210	an analytical subject	190:210	an analytical subject of great interest	190:228	Currently, detailed structural characterization of low-molecular-weight heparin (LMWH) products is an analytical subject of great interest.
28707621	3	38	theme	drugs	848:852	arg1	analysis					827:834	the detailed disaccharide analysis	801:834	the detailed disaccharide analysis of new LMWH drugs	801:852	For this purpose, we used strong anion-exchange (SAX) chromatography with spectrophotometric detection because this method is more helpful, easier, and faster than other separation techniques for the detailed disaccharide analysis of new LMWH drugs.
28707621	4	39	theme	hoc	974:976	arg1	test					978:981	Newman-Keuls post hoc test	956:981	Newman-Keuls post hoc test	956:981	In addition, we subjected the obtained results to statistical analysis (factor analysis, t-test, and Newman-Keuls post hoc test).
28707621	3	40	theme	anion-exchange	638:651	arg1	chromatography					659:672	strong anion-exchange (SAX) chromatography	631:672	strong anion-exchange (SAX) chromatography with spectrophotometric detection	631:706	For this purpose, we used strong anion-exchange (SAX) chromatography with spectrophotometric detection because this method is more helpful, easier, and faster than other separation techniques for the detailed disaccharide analysis of new LMWH drugs.
28707621	2	41	theme	structural	276:285	arg1	analysis					287:294	a comprehensive structural analysis	260:294	a comprehensive structural analysis of LMWHs	260:303	In this work, we carried out a comprehensive structural analysis of LMWHs and applied a modified pharmacopeial method, as well as methods developed by other researchers, to the analysis of novel biosimilar LMWH products; and, for the first time, compared the qualitative and quantitative composition of commercially available drugs (enoxaparin, nadroparin, and dalteparin).
28707621	2	42	theme	comprehensive	262:274	arg1	analysis					287:294	a comprehensive structural analysis	260:294	a comprehensive structural analysis of LMWHs	260:303	In this work, we carried out a comprehensive structural analysis of LMWHs and applied a modified pharmacopeial method, as well as methods developed by other researchers, to the analysis of novel biosimilar LMWH products; and, for the first time, compared the qualitative and quantitative composition of commercially available drugs (enoxaparin, nadroparin, and dalteparin).
28707621	2	43	theme	other	382:386	arg1	researchers					388:398	other researchers	382:398	other researchers	382:398	In this work, we carried out a comprehensive structural analysis of LMWHs and applied a modified pharmacopeial method, as well as methods developed by other researchers, to the analysis of novel biosimilar LMWH products; and, for the first time, compared the qualitative and quantitative composition of commercially available drugs (enoxaparin, nadroparin, and dalteparin).
28707621	3	44	theme	new	839:841	arg1	drugs					848:852	new LMWH drugs	839:852	new LMWH drugs	839:852	For this purpose, we used strong anion-exchange (SAX) chromatography with spectrophotometric detection because this method is more helpful, easier, and faster than other separation techniques for the detailed disaccharide analysis of new LMWH drugs.
28707621	2	45	contain	carried	248:254	arg2	analysis					287:294	a comprehensive structural analysis	260:294	a comprehensive structural analysis of LMWHs	260:303	In this work, we carried out a comprehensive structural analysis of LMWHs and applied a modified pharmacopeial method, as well as methods developed by other researchers, to the analysis of novel biosimilar LMWH products; and, for the first time, compared the qualitative and quantitative composition of commercially available drugs (enoxaparin, nadroparin, and dalteparin).
28707621	2	45	contain	carried	248:254	arg3	work					239:242	this work	234:242	this work	234:242	In this work, we carried out a comprehensive structural analysis of LMWHs and applied a modified pharmacopeial method, as well as methods developed by other researchers, to the analysis of novel biosimilar LMWH products; and, for the first time, compared the qualitative and quantitative composition of commercially available drugs (enoxaparin, nadroparin, and dalteparin).
28707621	2	45	contain	carried	248:254	arg1	we					245:246	we	245:246	we	245:246	In this work, we carried out a comprehensive structural analysis of LMWHs and applied a modified pharmacopeial method, as well as methods developed by other researchers, to the analysis of novel biosimilar LMWH products; and, for the first time, compared the qualitative and quantitative composition of commercially available drugs (enoxaparin, nadroparin, and dalteparin).
28707621	3	46	theme	spectrophotometric	679:696	arg1	detection					698:706	spectrophotometric detection	679:706	spectrophotometric detection	679:706	For this purpose, we used strong anion-exchange (SAX) chromatography with spectrophotometric detection because this method is more helpful, easier, and faster than other separation techniques for the detailed disaccharide analysis of new LMWH drugs.
28707621	0	47	theme	Low-Molecular-Weight	20:39	arg1	Heparins					41:48	Low-Molecular-Weight Heparins	20:48	Low-Molecular-Weight Heparins	20:48	Characterization of Low-Molecular-Weight Heparins by Strong Anion-Exchange Chromatography.
28707621	3	48	with	chromatography	659:672	arg1	detection					698:706	spectrophotometric detection	679:706	spectrophotometric detection	679:706	For this purpose, we used strong anion-exchange (SAX) chromatography with spectrophotometric detection because this method is more helpful, easier, and faster than other separation techniques for the detailed disaccharide analysis of new LMWH drugs.
28707621	3	49	theme	separation	775:784	arg1	techniques					786:795	other separation techniques	769:795	other separation techniques for the detailed disaccharide analysis of new LMWH drugs	769:852	For this purpose, we used strong anion-exchange (SAX) chromatography with spectrophotometric detection because this method is more helpful, easier, and faster than other separation techniques for the detailed disaccharide analysis of new LMWH drugs.
28732899	0	0	theme	Enhancing	87:95	arg1	antimicrobial					97:109	Enhancing antimicrobial	87:109	Enhancing antimicrobial	87:109	In situ reduction of silver nanoparticles by chitosan-l-glutamic acid/hyaluronic acid: Enhancing antimicrobial and wound-healing activity.
28732899	6	1	contain	containing	847:856	arg1	composites					836:845	Spongy composites	829:845	Spongy composites containing low concentrations of AgNP	829:883	Spongy composites containing low concentrations of AgNP were non-toxic to L929 cells, while CG/HA/AgNP spongy composites promoted wound healing, as determined by in vivo tests, wound contraction ratio, average healing time, and histological examination.
28732899	6	1	contain	containing	847:856	arg2	concentrations					862:875	low concentrations	858:875	low concentrations of AgNP	858:883	Spongy composites containing low concentrations of AgNP were non-toxic to L929 cells, while CG/HA/AgNP spongy composites promoted wound healing, as determined by in vivo tests, wound contraction ratio, average healing time, and histological examination.
28732899	5	2	theme	In	654:655	arg1	activity					677:684	In vitro antibacterial activity	654:684	In vitro antibacterial activity	654:684	In vitro antibacterial activity showed that the CG/AgNP spongy composites effectively inhibited bacterial (Escherichia coli and Staphylococcus aureus) growth and penetration.
28732899	2	3	theme	CG/AgNP	349:355	arg1	composites					364:373	CG/AgNP spongy composites	349:373	CG/AgNP spongy composites	349:373	CG/AgNP spongy composites had an interconnected porous structure and rough surfaces.
28732899	4	4	theme	good	586:589	arg1	properties					602:611	good mechanical properties	586:611	good mechanical properties	586:611	The spongy composites exhibited good mechanical properties, swelling, and water retention capacity.
28732899	6	5	theme	contraction	1012:1022	arg1	ratio					1024:1028	wound contraction ratio	1006:1028	wound contraction ratio	1006:1028	Spongy composites containing low concentrations of AgNP were non-toxic to L929 cells, while CG/HA/AgNP spongy composites promoted wound healing, as determined by in vivo tests, wound contraction ratio, average healing time, and histological examination.
28732899	7	6	theme	antibacterial	1156:1168	arg1	composites					1122:1131	the spongy composites	1111:1131	the spongy composites	1111:1131	These results indicate that the spongy composites can serve as effective antibacterial wound dressings.
28732899	7	6	theme	antibacterial	1156:1168	arg1	dressings					1176:1184	effective antibacterial wound dressings	1146:1184	effective antibacterial wound dressings	1146:1184	These results indicate that the spongy composites can serve as effective antibacterial wound dressings.
28732899	6	7	theme	low	858:860	arg1	concentrations					862:875	low concentrations	858:875	low concentrations of AgNP	858:883	Spongy composites containing low concentrations of AgNP were non-toxic to L929 cells, while CG/HA/AgNP spongy composites promoted wound healing, as determined by in vivo tests, wound contraction ratio, average healing time, and histological examination.
28732899	3	8	theme	AgNP	499:502	arg1	aggregation					504:514	AgNP aggregation	499:514	AgNP aggregation	499:514	When AgNPs (5-20nm) were immobilized on these spongy composites, AgNP aggregation was dependent on AgNO3 concentration.
28732899	7	9	theme	effective	1146:1154	arg1	composites					1122:1131	the spongy composites	1111:1131	the spongy composites	1111:1131	These results indicate that the spongy composites can serve as effective antibacterial wound dressings.
28732899	7	9	theme	effective	1146:1154	arg1	dressings					1176:1184	effective antibacterial wound dressings	1146:1184	effective antibacterial wound dressings	1146:1184	These results indicate that the spongy composites can serve as effective antibacterial wound dressings.
28732899	5	10	theme	bacterial	750:758	arg1	growth					805:810	bacterial (Escherichia coli and Staphylococcus aureus) growth	750:810	bacterial (Escherichia coli and Staphylococcus aureus) growth	750:810	In vitro antibacterial activity showed that the CG/AgNP spongy composites effectively inhibited bacterial (Escherichia coli and Staphylococcus aureus) growth and penetration.
28732899	5	11	theme	antibacterial	663:675	arg1	activity					677:684	In vitro antibacterial activity	654:684	In vitro antibacterial activity	654:684	In vitro antibacterial activity showed that the CG/AgNP spongy composites effectively inhibited bacterial (Escherichia coli and Staphylococcus aureus) growth and penetration.
28732899	3	12	theme	AgNO3	533:537	arg1	concentration					539:551	AgNO3 concentration	533:551	AgNO3 concentration	533:551	When AgNPs (5-20nm) were immobilized on these spongy composites, AgNP aggregation was dependent on AgNO3 concentration.
28732899	1	13	theme	chitosan-l-glutamic	265:283	arg1	derivative					295:304	chitosan-l-glutamic acid (CG) derivative	265:304	chitosan-l-glutamic acid (CG) derivative loaded with hyaluronic acid (HA) solution	265:346	Spongy composites with silver nanoparticles (AgNPs) were synthesized by freeze-drying a mixture of silver nitrate (AgNO3) and chitosan-l-glutamic acid (CG) derivative loaded with hyaluronic acid (HA) solution.
28732899	6	14	theme	spongy	932:937	arg1	composites					939:948	CG/HA/AgNP spongy composites	921:948	CG/HA/AgNP spongy composites	921:948	Spongy composites containing low concentrations of AgNP were non-toxic to L929 cells, while CG/HA/AgNP spongy composites promoted wound healing, as determined by in vivo tests, wound contraction ratio, average healing time, and histological examination.
28732899	6	15	theme	wound	1006:1010	arg1	ratio					1024:1028	wound contraction ratio	1006:1028	wound contraction ratio	1006:1028	Spongy composites containing low concentrations of AgNP were non-toxic to L929 cells, while CG/HA/AgNP spongy composites promoted wound healing, as determined by in vivo tests, wound contraction ratio, average healing time, and histological examination.
28732899	1	16	theme	acid	285:288	arg1	derivative					295:304	chitosan-l-glutamic acid (CG) derivative	265:304	chitosan-l-glutamic acid (CG) derivative loaded with hyaluronic acid (HA) solution	265:346	Spongy composites with silver nanoparticles (AgNPs) were synthesized by freeze-drying a mixture of silver nitrate (AgNO3) and chitosan-l-glutamic acid (CG) derivative loaded with hyaluronic acid (HA) solution.
28732899	6	17	theme	CG/HA/AgNP	921:930	arg1	composites					939:948	CG/HA/AgNP spongy composites	921:948	CG/HA/AgNP spongy composites	921:948	Spongy composites containing low concentrations of AgNP were non-toxic to L929 cells, while CG/HA/AgNP spongy composites promoted wound healing, as determined by in vivo tests, wound contraction ratio, average healing time, and histological examination.
28732899	1	18	with	composites	146:155	arg1	nanoparticles					169:181	silver nanoparticles	162:181	silver nanoparticles (AgNPs)	162:189	Spongy composites with silver nanoparticles (AgNPs) were synthesized by freeze-drying a mixture of silver nitrate (AgNO3) and chitosan-l-glutamic acid (CG) derivative loaded with hyaluronic acid (HA) solution.
28732899	1	18	with	composites	146:155	arg1	AgNPs					184:188	AgNPs	184:188	AgNPs	184:188	Spongy composites with silver nanoparticles (AgNPs) were synthesized by freeze-drying a mixture of silver nitrate (AgNO3) and chitosan-l-glutamic acid (CG) derivative loaded with hyaluronic acid (HA) solution.
28732899	4	19	theme	water	628:632	arg1	capacity					644:651	water retention capacity	628:651	water retention capacity	628:651	The spongy composites exhibited good mechanical properties, swelling, and water retention capacity.
28732899	2	20	theme	porous	397:402	arg1	structure					404:412	an interconnected porous structure	379:412	an interconnected porous structure	379:412	CG/AgNP spongy composites had an interconnected porous structure and rough surfaces.
28732899	0	21	theme	wound-healing	115:127	arg1	activity					129:136	wound-healing activity	115:136	wound-healing activity	115:136	In situ reduction of silver nanoparticles by chitosan-l-glutamic acid/hyaluronic acid: Enhancing antimicrobial and wound-healing activity.
28732899	7	22	theme	wound	1170:1174	arg1	composites					1122:1131	the spongy composites	1111:1131	the spongy composites	1111:1131	These results indicate that the spongy composites can serve as effective antibacterial wound dressings.
28732899	7	22	theme	wound	1170:1174	arg1	dressings					1176:1184	effective antibacterial wound dressings	1146:1184	effective antibacterial wound dressings	1146:1184	These results indicate that the spongy composites can serve as effective antibacterial wound dressings.
28732899	2	23	theme	interconnected	382:395	arg1	structure					404:412	an interconnected porous structure	379:412	an interconnected porous structure	379:412	CG/AgNP spongy composites had an interconnected porous structure and rough surfaces.
28732899	6	24	theme	in	991:992	arg1	tests					999:1003	in vivo tests	991:1003	in vivo tests	991:1003	Spongy composites containing low concentrations of AgNP were non-toxic to L929 cells, while CG/HA/AgNP spongy composites promoted wound healing, as determined by in vivo tests, wound contraction ratio, average healing time, and histological examination.
28732899	5	25	dep	growth	805:810	arg1	coli					773:776	Escherichia coli	761:776	Escherichia coli	761:776	In vitro antibacterial activity showed that the CG/AgNP spongy composites effectively inhibited bacterial (Escherichia coli and Staphylococcus aureus) growth and penetration.
28732899	5	25	dep	growth	805:810	arg1	aureus					797:802	Staphylococcus aureus	782:802	Staphylococcus aureus	782:802	In vitro antibacterial activity showed that the CG/AgNP spongy composites effectively inhibited bacterial (Escherichia coli and Staphylococcus aureus) growth and penetration.
28732899	0	26	theme	In	0:1	arg1	reduction					8:16	In situ reduction	0:16	In situ reduction of silver nanoparticles by chitosan-l-glutamic acid/hyaluronic acid: Enhancing antimicrobial and wound-healing activity.	0:137	In situ reduction of silver nanoparticles by chitosan-l-glutamic acid/hyaluronic acid: Enhancing antimicrobial and wound-healing activity.
28732899	7	27	theme	spongy	1115:1120	arg1	composites					1122:1131	the spongy composites	1111:1131	the spongy composites	1111:1131	These results indicate that the spongy composites can serve as effective antibacterial wound dressings.
28732899	7	27	theme	spongy	1115:1120	arg1	dressings					1176:1184	effective antibacterial wound dressings	1146:1184	effective antibacterial wound dressings	1146:1184	These results indicate that the spongy composites can serve as effective antibacterial wound dressings.
28732899	5	28	dep	In	654:655	arg1	vitro					657:661	vitro	657:661	vitro	657:661	In vitro antibacterial activity showed that the CG/AgNP spongy composites effectively inhibited bacterial (Escherichia coli and Staphylococcus aureus) growth and penetration.
28732899	1	29	theme	CG	291:292	arg1	derivative					295:304	chitosan-l-glutamic acid (CG) derivative	265:304	chitosan-l-glutamic acid (CG) derivative loaded with hyaluronic acid (HA) solution	265:346	Spongy composites with silver nanoparticles (AgNPs) were synthesized by freeze-drying a mixture of silver nitrate (AgNO3) and chitosan-l-glutamic acid (CG) derivative loaded with hyaluronic acid (HA) solution.
28732899	5	30	theme	spongy	710:715	arg1	composites					717:726	the CG/AgNP spongy composites	698:726	the CG/AgNP spongy composites	698:726	In vitro antibacterial activity showed that the CG/AgNP spongy composites effectively inhibited bacterial (Escherichia coli and Staphylococcus aureus) growth and penetration.
28732899	6	31	theme	Spongy	829:834	arg1	composites					836:845	Spongy composites	829:845	Spongy composites containing low concentrations of AgNP	829:883	Spongy composites containing low concentrations of AgNP were non-toxic to L929 cells, while CG/HA/AgNP spongy composites promoted wound healing, as determined by in vivo tests, wound contraction ratio, average healing time, and histological examination.
28732899	4	32	theme	retention	634:642	arg1	capacity					644:651	water retention capacity	628:651	water retention capacity	628:651	The spongy composites exhibited good mechanical properties, swelling, and water retention capacity.
28732899	2	33	contain	had	375:377	arg2	surfaces					424:431	rough surfaces	418:431	rough surfaces	418:431	CG/AgNP spongy composites had an interconnected porous structure and rough surfaces.
28732899	2	33	contain	had	375:377	arg2	structure					404:412	an interconnected porous structure	379:412	an interconnected porous structure	379:412	CG/AgNP spongy composites had an interconnected porous structure and rough surfaces.
28732899	2	33	contain	had	375:377	arg1	composites					364:373	CG/AgNP spongy composites	349:373	CG/AgNP spongy composites	349:373	CG/AgNP spongy composites had an interconnected porous structure and rough surfaces.
28732899	6	34	theme	wound	959:963	arg1	healing					965:971	wound healing	959:971	wound healing	959:971	Spongy composites containing low concentrations of AgNP were non-toxic to L929 cells, while CG/HA/AgNP spongy composites promoted wound healing, as determined by in vivo tests, wound contraction ratio, average healing time, and histological examination.
28732899	2	35	theme	rough	418:422	arg1	surfaces					424:431	rough surfaces	418:431	rough surfaces	418:431	CG/AgNP spongy composites had an interconnected porous structure and rough surfaces.
28732899	0	36	theme	nanoparticles	28:40	arg1	reduction					8:16	In situ reduction	0:16	In situ reduction of silver nanoparticles by chitosan-l-glutamic acid/hyaluronic acid: Enhancing antimicrobial and wound-healing activity.	0:137	In situ reduction of silver nanoparticles by chitosan-l-glutamic acid/hyaluronic acid: Enhancing antimicrobial and wound-healing activity.
28732899	4	37	theme	spongy	558:563	arg1	composites					565:574	The spongy composites	554:574	The spongy composites	554:574	The spongy composites exhibited good mechanical properties, swelling, and water retention capacity.
28732899	6	38	theme	L929	903:906	arg1	cells					908:912	L929 cells	903:912	L929 cells	903:912	Spongy composites containing low concentrations of AgNP were non-toxic to L929 cells, while CG/HA/AgNP spongy composites promoted wound healing, as determined by in vivo tests, wound contraction ratio, average healing time, and histological examination.
28732899	6	39	theme	histological	1057:1068	arg1	examination					1070:1080	histological examination	1057:1080	histological examination	1057:1080	Spongy composites containing low concentrations of AgNP were non-toxic to L929 cells, while CG/HA/AgNP spongy composites promoted wound healing, as determined by in vivo tests, wound contraction ratio, average healing time, and histological examination.
28732899	0	40	theme	silver	21:26	arg1	nanoparticles					28:40	silver nanoparticles	21:40	silver nanoparticles	21:40	In situ reduction of silver nanoparticles by chitosan-l-glutamic acid/hyaluronic acid: Enhancing antimicrobial and wound-healing activity.
28732899	6	41	theme	healing	1039:1045	arg1	time					1047:1050	average healing time	1031:1050	average healing time	1031:1050	Spongy composites containing low concentrations of AgNP were non-toxic to L929 cells, while CG/HA/AgNP spongy composites promoted wound healing, as determined by in vivo tests, wound contraction ratio, average healing time, and histological examination.
28732899	0	42	theme	chitosan-l-glutamic	45:63	arg1	acid					81:84	chitosan-l-glutamic acid/hyaluronic acid	45:84	chitosan-l-glutamic acid/hyaluronic acid	45:84	In situ reduction of silver nanoparticles by chitosan-l-glutamic acid/hyaluronic acid: Enhancing antimicrobial and wound-healing activity.
28732899	3	43	theme	spongy	480:485	arg1	composites					487:496	these spongy composites	474:496	these spongy composites	474:496	When AgNPs (5-20nm) were immobilized on these spongy composites, AgNP aggregation was dependent on AgNO3 concentration.
28732899	1	44	theme	hyaluronic	318:327	arg1	HA					335:336	HA	335:336	HA	335:336	Spongy composites with silver nanoparticles (AgNPs) were synthesized by freeze-drying a mixture of silver nitrate (AgNO3) and chitosan-l-glutamic acid (CG) derivative loaded with hyaluronic acid (HA) solution.
28732899	1	44	theme	hyaluronic	318:327	arg1	acid					329:332	hyaluronic acid	318:332	hyaluronic acid (HA) solution	318:346	Spongy composites with silver nanoparticles (AgNPs) were synthesized by freeze-drying a mixture of silver nitrate (AgNO3) and chitosan-l-glutamic acid (CG) derivative loaded with hyaluronic acid (HA) solution.
28732899	6	45	dep	in	991:992	arg1	vivo					994:997	vivo	994:997	vivo	994:997	Spongy composites containing low concentrations of AgNP were non-toxic to L929 cells, while CG/HA/AgNP spongy composites promoted wound healing, as determined by in vivo tests, wound contraction ratio, average healing time, and histological examination.
28732899	1	46	theme	Spongy	139:144	arg1	composites					146:155	Spongy composites	139:155	Spongy composites with silver nanoparticles (AgNPs)	139:189	Spongy composites with silver nanoparticles (AgNPs) were synthesized by freeze-drying a mixture of silver nitrate (AgNO3) and chitosan-l-glutamic acid (CG) derivative loaded with hyaluronic acid (HA) solution.
28732899	1	47	theme	acid	329:332	arg1	solution					339:346	hyaluronic acid (HA) solution	318:346	hyaluronic acid (HA) solution	318:346	Spongy composites with silver nanoparticles (AgNPs) were synthesized by freeze-drying a mixture of silver nitrate (AgNO3) and chitosan-l-glutamic acid (CG) derivative loaded with hyaluronic acid (HA) solution.
28732899	6	48	theme	AgNP	880:883	arg1	concentrations					862:875	low concentrations	858:875	low concentrations of AgNP	858:883	Spongy composites containing low concentrations of AgNP were non-toxic to L929 cells, while CG/HA/AgNP spongy composites promoted wound healing, as determined by in vivo tests, wound contraction ratio, average healing time, and histological examination.
28732899	1	49	theme	silver	238:243	arg1	AgNO3					254:258	AgNO3	254:258	AgNO3	254:258	Spongy composites with silver nanoparticles (AgNPs) were synthesized by freeze-drying a mixture of silver nitrate (AgNO3) and chitosan-l-glutamic acid (CG) derivative loaded with hyaluronic acid (HA) solution.
28732899	1	49	theme	silver	238:243	arg1	nitrate					245:251	silver nitrate	238:251	silver nitrate (AgNO3)	238:259	Spongy composites with silver nanoparticles (AgNPs) were synthesized by freeze-drying a mixture of silver nitrate (AgNO3) and chitosan-l-glutamic acid (CG) derivative loaded with hyaluronic acid (HA) solution.
28732899	5	50	theme	CG/AgNP	702:708	arg1	composites					717:726	the CG/AgNP spongy composites	698:726	the CG/AgNP spongy composites	698:726	In vitro antibacterial activity showed that the CG/AgNP spongy composites effectively inhibited bacterial (Escherichia coli and Staphylococcus aureus) growth and penetration.
28732899	0	51	dep	reduction	8:16	arg1	antimicrobial					97:109	Enhancing antimicrobial	87:109	Enhancing antimicrobial	87:109	In situ reduction of silver nanoparticles by chitosan-l-glutamic acid/hyaluronic acid: Enhancing antimicrobial and wound-healing activity.
28732899	0	51	dep	reduction	8:16	arg1	activity					129:136	wound-healing activity	115:136	wound-healing activity	115:136	In situ reduction of silver nanoparticles by chitosan-l-glutamic acid/hyaluronic acid: Enhancing antimicrobial and wound-healing activity.
28732899	1	52	theme	nitrate	245:251	arg1	derivative					295:304	chitosan-l-glutamic acid (CG) derivative	265:304	chitosan-l-glutamic acid (CG) derivative loaded with hyaluronic acid (HA) solution	265:346	Spongy composites with silver nanoparticles (AgNPs) were synthesized by freeze-drying a mixture of silver nitrate (AgNO3) and chitosan-l-glutamic acid (CG) derivative loaded with hyaluronic acid (HA) solution.
28732899	1	52	theme	nitrate	245:251	arg1	mixture					227:233	a mixture	225:233	a mixture of silver nitrate (AgNO3)	225:259	Spongy composites with silver nanoparticles (AgNPs) were synthesized by freeze-drying a mixture of silver nitrate (AgNO3) and chitosan-l-glutamic acid (CG) derivative loaded with hyaluronic acid (HA) solution.
28732899	1	53	theme	silver	162:167	arg1	nanoparticles					169:181	silver nanoparticles	162:181	silver nanoparticles (AgNPs)	162:189	Spongy composites with silver nanoparticles (AgNPs) were synthesized by freeze-drying a mixture of silver nitrate (AgNO3) and chitosan-l-glutamic acid (CG) derivative loaded with hyaluronic acid (HA) solution.
28732899	1	53	theme	silver	162:167	arg1	AgNPs					184:188	AgNPs	184:188	AgNPs	184:188	Spongy composites with silver nanoparticles (AgNPs) were synthesized by freeze-drying a mixture of silver nitrate (AgNO3) and chitosan-l-glutamic acid (CG) derivative loaded with hyaluronic acid (HA) solution.
28732899	4	54	theme	mechanical	591:600	arg1	properties					602:611	good mechanical properties	586:611	good mechanical properties	586:611	The spongy composites exhibited good mechanical properties, swelling, and water retention capacity.
28732899	0	55	theme	acid/hyaluronic	65:79	arg1	acid					81:84	chitosan-l-glutamic acid/hyaluronic acid	45:84	chitosan-l-glutamic acid/hyaluronic acid	45:84	In situ reduction of silver nanoparticles by chitosan-l-glutamic acid/hyaluronic acid: Enhancing antimicrobial and wound-healing activity.
28732899	0	56	dep	In	0:1	arg1	situ					3:6	situ	3:6	situ	3:6	In situ reduction of silver nanoparticles by chitosan-l-glutamic acid/hyaluronic acid: Enhancing antimicrobial and wound-healing activity.
28732899	2	57	theme	spongy	357:362	arg1	composites					364:373	CG/AgNP spongy composites	349:373	CG/AgNP spongy composites	349:373	CG/AgNP spongy composites had an interconnected porous structure and rough surfaces.
28732899	6	58	theme	average	1031:1037	arg1	time					1047:1050	average healing time	1031:1050	average healing time	1031:1050	Spongy composites containing low concentrations of AgNP were non-toxic to L929 cells, while CG/HA/AgNP spongy composites promoted wound healing, as determined by in vivo tests, wound contraction ratio, average healing time, and histological examination.
28109811	0	0	theme	chicken	91:97	arg1	comb					99:102	chicken comb	91:102	chicken comb	91:102	Production and characterization of bacterial cellulose membranes with hyaluronic acid from chicken comb.
28109811	6	1	theme	similar	1075:1081	arg1	characteristics					1059:1073	structural characteristics	1048:1073	structural characteristics similar to the standard commercial hyaluronic acid	1048:1124	The optimized process of extraction and purification of hyaluronic acid resulted in low molar mass hyaluronic acid with structural characteristics similar to the standard commercial hyaluronic acid.
28109811	3	2	attach	isolated	553:560	arg1	comb					575:578	chicken comb	567:578	chicken comb on different days of fermentation, specifically on the first (BCHA-SABT0) and third day (BCHA-SABT3) of fermentation	567:695	In this study, polymeric composites were produced in presence of hyaluronic acid isolated from chicken comb on different days of fermentation, specifically on the first (BCHA-SABT0) and third day (BCHA-SABT3) of fermentation.
28109811	3	2	attach	isolated	553:560	arg2	acid					548:551	hyaluronic acid	537:551	hyaluronic acid isolated from chicken comb on different days of fermentation, specifically on the first (BCHA-SABT0) and third day (BCHA-SABT3) of fermentation	537:695	In this study, polymeric composites were produced in presence of hyaluronic acid isolated from chicken comb on different days of fermentation, specifically on the first (BCHA-SABT0) and third day (BCHA-SABT3) of fermentation.
28109811	6	3	theme	purification	968:979	arg1	process					942:948	The optimized process	928:948	The optimized process of extraction and purification of hyaluronic acid	928:998	The optimized process of extraction and purification of hyaluronic acid resulted in low molar mass hyaluronic acid with structural characteristics similar to the standard commercial hyaluronic acid.
28109811	8	4	theme	HA-SAB	1292:1297	arg1	incorporation					1275:1287	better incorporation	1268:1287	better incorporation of HA-SAB between cellulose microfiber	1268:1326	The membranes produced on the third day presented better incorporation of HA-SAB between cellulose microfiber, resulting in membranes with higher thermal stability, higher roughness and lower crystallinity.
28109811	9	5	theme	cellulose	1458:1466	arg1	biocompatiblily					1429:1443	The biocompatiblily	1425:1443	The biocompatiblily of bacterial cellulose	1425:1466	The biocompatiblily of bacterial cellulose and the importance of hyaluronic acid as a component of extracellular matrix qualify the polymeric composites as promising biomaterials for tissue engineering.
28109811	9	5	theme	cellulose	1458:1466	arg1	importance					1476:1485	the importance	1472:1485	the importance of hyaluronic acid as a component of extracellular matrix	1472:1543	The biocompatiblily of bacterial cellulose and the importance of hyaluronic acid as a component of extracellular matrix qualify the polymeric composites as promising biomaterials for tissue engineering.
28109811	1	6	from	Gluconacetobacter	140:156	arg1	BC					130:131	BC	130:131	BC	130:131	The bacterial cellulose (BC), from Gluconacetobacter hansenii, is a biofilm with a high degree of crystallinity that can be used for therapeutic purposes and as a candidate for healing wounds.
28109811	1	6	from	Gluconacetobacter	140:156	arg1	cellulose					119:127	The bacterial cellulose	105:127	The bacterial cellulose (BC)	105:132	The bacterial cellulose (BC), from Gluconacetobacter hansenii, is a biofilm with a high degree of crystallinity that can be used for therapeutic purposes and as a candidate for healing wounds.
28109811	1	6	from	Gluconacetobacter	140:156	arg1	biofilm					173:179	a biofilm	171:179	a biofilm with a high degree of crystallinity that can be used for therapeutic purposes and as a candidate for healing wounds	171:295	The bacterial cellulose (BC), from Gluconacetobacter hansenii, is a biofilm with a high degree of crystallinity that can be used for therapeutic purposes and as a candidate for healing wounds.
28109811	8	7	theme	cellulose	1307:1315	arg1	microfiber					1317:1326	cellulose microfiber	1307:1326	cellulose microfiber	1307:1326	The membranes produced on the third day presented better incorporation of HA-SAB between cellulose microfiber, resulting in membranes with higher thermal stability, higher roughness and lower crystallinity.
28109811	9	8	theme	hyaluronic	1490:1499	arg1	acid					1501:1504	hyaluronic acid	1490:1504	hyaluronic acid	1490:1504	The biocompatiblily of bacterial cellulose and the importance of hyaluronic acid as a component of extracellular matrix qualify the polymeric composites as promising biomaterials for tissue engineering.
28109811	3	9	from	days	593:596	arg1	day					664:666	the first (BCHA-SABT0) and third day	631:666	the first (BCHA-SABT0) and third day (BCHA-SABT3) of fermentation	631:695	In this study, polymeric composites were produced in presence of hyaluronic acid isolated from chicken comb on different days of fermentation, specifically on the first (BCHA-SABT0) and third day (BCHA-SABT3) of fermentation.
28109811	3	9	from	days	593:596	arg1	BCHA-SABT3					669:678	BCHA-SABT3	669:678	BCHA-SABT3	669:678	In this study, polymeric composites were produced in presence of hyaluronic acid isolated from chicken comb on different days of fermentation, specifically on the first (BCHA-SABT0) and third day (BCHA-SABT3) of fermentation.
28109811	4	10	theme	thermal	730:736	arg1	stability					738:746	thermal stability	730:746	thermal stability	730:746	The structural characteristics, thermal stability and molar mass of hyaluronic acid from chicken comb were evaluated.
28109811	4	11	theme	acid	777:780	arg1	characteristics					713:727	The structural characteristics	698:727	The structural characteristics	698:727	The structural characteristics, thermal stability and molar mass of hyaluronic acid from chicken comb were evaluated.
28109811	4	11	theme	acid	777:780	arg1	stability					738:746	thermal stability	730:746	thermal stability	730:746	The structural characteristics, thermal stability and molar mass of hyaluronic acid from chicken comb were evaluated.
28109811	4	11	theme	acid	777:780	arg1	mass					758:761	molar mass	752:761	molar mass	752:761	The structural characteristics, thermal stability and molar mass of hyaluronic acid from chicken comb were evaluated.
28109811	4	12	theme	structural	702:711	arg1	characteristics					713:727	The structural characteristics	698:727	The structural characteristics	698:727	The structural characteristics, thermal stability and molar mass of hyaluronic acid from chicken comb were evaluated.
28109811	8	13	theme	thermal	1364:1370	arg1	stability					1372:1380	higher thermal stability	1357:1380	higher thermal stability	1357:1380	The membranes produced on the third day presented better incorporation of HA-SAB between cellulose microfiber, resulting in membranes with higher thermal stability, higher roughness and lower crystallinity.
28109811	6	14	theme	extraction	953:962	arg1	process					942:948	The optimized process	928:948	The optimized process of extraction and purification of hyaluronic acid	928:998	The optimized process of extraction and purification of hyaluronic acid resulted in low molar mass hyaluronic acid with structural characteristics similar to the standard commercial hyaluronic acid.
28109811	6	15	theme	optimized	932:940	arg1	process					942:948	The optimized process	928:948	The optimized process of extraction and purification of hyaluronic acid	928:998	The optimized process of extraction and purification of hyaluronic acid resulted in low molar mass hyaluronic acid with structural characteristics similar to the standard commercial hyaluronic acid.
28109811	4	16	from	characteristics	713:727	arg1	comb					795:798	chicken comb	787:798	chicken comb	787:798	The structural characteristics, thermal stability and molar mass of hyaluronic acid from chicken comb were evaluated.
28109811	9	17	theme	extracellular	1524:1536	arg1	matrix					1538:1543	extracellular matrix	1524:1543	extracellular matrix	1524:1543	The biocompatiblily of bacterial cellulose and the importance of hyaluronic acid as a component of extracellular matrix qualify the polymeric composites as promising biomaterials for tissue engineering.
28109811	1	18	theme	therapeutic	238:248	arg1	purposes					250:257	therapeutic purposes	238:257	therapeutic purposes	238:257	The bacterial cellulose (BC), from Gluconacetobacter hansenii, is a biofilm with a high degree of crystallinity that can be used for therapeutic purposes and as a candidate for healing wounds.
28109811	4	19	from	comb	795:798	arg1	characteristics					713:727	The structural characteristics	698:727	The structural characteristics	698:727	The structural characteristics, thermal stability and molar mass of hyaluronic acid from chicken comb were evaluated.
28109811	4	19	from	comb	795:798	arg1	stability					738:746	thermal stability	730:746	thermal stability	730:746	The structural characteristics, thermal stability and molar mass of hyaluronic acid from chicken comb were evaluated.
28109811	4	19	from	comb	795:798	arg1	mass					758:761	molar mass	752:761	molar mass	752:761	The structural characteristics, thermal stability and molar mass of hyaluronic acid from chicken comb were evaluated.
28109811	4	19	from	comb	795:798	arg1	acid					777:780	hyaluronic acid	766:780	hyaluronic acid from chicken comb	766:798	The structural characteristics, thermal stability and molar mass of hyaluronic acid from chicken comb were evaluated.
28109811	4	20	theme	chicken	787:793	arg1	comb					795:798	chicken comb	787:798	chicken comb	787:798	The structural characteristics, thermal stability and molar mass of hyaluronic acid from chicken comb were evaluated.
28109811	6	21	dep	mass	1022:1025	arg1	acid					1038:1041	hyaluronic acid	1027:1041	low molar mass hyaluronic acid with structural characteristics similar to the standard commercial hyaluronic acid	1012:1124	The optimized process of extraction and purification of hyaluronic acid resulted in low molar mass hyaluronic acid with structural characteristics similar to the standard commercial hyaluronic acid.
28109811	3	22	from	comb	575:578	arg1	days					593:596	different days	583:596	different days of fermentation	583:612	In this study, polymeric composites were produced in presence of hyaluronic acid isolated from chicken comb on different days of fermentation, specifically on the first (BCHA-SABT0) and third day (BCHA-SABT3) of fermentation.
28109811	6	23	theme	structural	1048:1057	arg1	characteristics					1059:1073	structural characteristics	1048:1073	structural characteristics similar to the standard commercial hyaluronic acid	1048:1124	The optimized process of extraction and purification of hyaluronic acid resulted in low molar mass hyaluronic acid with structural characteristics similar to the standard commercial hyaluronic acid.
28109811	3	24	dep	first	635:639	arg1	BCHA-SABT0					642:651	BCHA-SABT0	642:651	BCHA-SABT0	642:651	In this study, polymeric composites were produced in presence of hyaluronic acid isolated from chicken comb on different days of fermentation, specifically on the first (BCHA-SABT0) and third day (BCHA-SABT3) of fermentation.
28109811	3	25	theme	third	658:662	arg1	day					664:666	the first (BCHA-SABT0) and third day	631:666	the first (BCHA-SABT0) and third day (BCHA-SABT3) of fermentation	631:695	In this study, polymeric composites were produced in presence of hyaluronic acid isolated from chicken comb on different days of fermentation, specifically on the first (BCHA-SABT0) and third day (BCHA-SABT3) of fermentation.
28109811	3	25	theme	third	658:662	arg1	BCHA-SABT3					669:678	BCHA-SABT3	669:678	BCHA-SABT3	669:678	In this study, polymeric composites were produced in presence of hyaluronic acid isolated from chicken comb on different days of fermentation, specifically on the first (BCHA-SABT0) and third day (BCHA-SABT3) of fermentation.
28109811	6	26	theme	commercial	1099:1108	arg1	acid					1121:1124	the standard commercial hyaluronic acid	1086:1124	the standard commercial hyaluronic acid	1086:1124	The optimized process of extraction and purification of hyaluronic acid resulted in low molar mass hyaluronic acid with structural characteristics similar to the standard commercial hyaluronic acid.
28109811	3	27	theme	polymeric	487:495	arg1	composites					497:506	polymeric composites	487:506	polymeric composites	487:506	In this study, polymeric composites were produced in presence of hyaluronic acid isolated from chicken comb on different days of fermentation, specifically on the first (BCHA-SABT0) and third day (BCHA-SABT3) of fermentation.
28109811	7	28	theme	polymeric	1160:1168	arg1	BC/HA-SAB					1182:1190	BC/HA-SAB	1182:1190	BC/HA-SAB	1182:1190	The results demonstrate that the polymeric composites (BC/HA-SAB) can be produced in situ.
28109811	7	28	theme	polymeric	1160:1168	arg1	composites					1170:1179	the polymeric composites	1156:1179	the polymeric composites (BC/HA-SAB)	1156:1191	The results demonstrate that the polymeric composites (BC/HA-SAB) can be produced in situ.
28109811	8	29	theme	lower	1404:1408	arg1	crystallinity					1410:1422	lower crystallinity	1404:1422	lower crystallinity	1404:1422	The membranes produced on the third day presented better incorporation of HA-SAB between cellulose microfiber, resulting in membranes with higher thermal stability, higher roughness and lower crystallinity.
28109811	8	30	theme	higher	1357:1362	arg1	stability					1372:1380	higher thermal stability	1357:1380	higher thermal stability	1357:1380	The membranes produced on the third day presented better incorporation of HA-SAB between cellulose microfiber, resulting in membranes with higher thermal stability, higher roughness and lower crystallinity.
28109811	5	31	theme	Native	816:821	arg1	membrane					823:830	Native membrane	816:830	Native membrane	816:830	Native membrane and polymeric composites were characterized with respect to their morphology and crystallinity.
28109811	6	32	theme	acid	995:998	arg1	purification					968:979	purification	968:979	purification	968:979	The optimized process of extraction and purification of hyaluronic acid resulted in low molar mass hyaluronic acid with structural characteristics similar to the standard commercial hyaluronic acid.
28109811	6	32	theme	acid	995:998	arg1	extraction					953:962	extraction	953:962	extraction	953:962	The optimized process of extraction and purification of hyaluronic acid resulted in low molar mass hyaluronic acid with structural characteristics similar to the standard commercial hyaluronic acid.
28109811	3	33	theme	fermentation	684:695	arg1	day					664:666	the first (BCHA-SABT0) and third day	631:666	the first (BCHA-SABT0) and third day (BCHA-SABT3) of fermentation	631:695	In this study, polymeric composites were produced in presence of hyaluronic acid isolated from chicken comb on different days of fermentation, specifically on the first (BCHA-SABT0) and third day (BCHA-SABT3) of fermentation.
28109811	3	33	theme	fermentation	684:695	arg1	BCHA-SABT3					669:678	BCHA-SABT3	669:678	BCHA-SABT3	669:678	In this study, polymeric composites were produced in presence of hyaluronic acid isolated from chicken comb on different days of fermentation, specifically on the first (BCHA-SABT0) and third day (BCHA-SABT3) of fermentation.
28109811	9	34	theme	promising	1581:1589	arg1	composites					1567:1576	the polymeric composites	1553:1576	the polymeric composites	1553:1576	The biocompatiblily of bacterial cellulose and the importance of hyaluronic acid as a component of extracellular matrix qualify the polymeric composites as promising biomaterials for tissue engineering.
28109811	9	34	theme	promising	1581:1589	arg1	biomaterials					1591:1602	promising biomaterials	1581:1602	promising biomaterials for tissue engineering	1581:1625	The biocompatiblily of bacterial cellulose and the importance of hyaluronic acid as a component of extracellular matrix qualify the polymeric composites as promising biomaterials for tissue engineering.
28109811	2	35	theme	constitutive	324:335	arg1	acid					309:312	Hyaluronic acid	298:312	Hyaluronic acid (HA)	298:317	Hyaluronic acid (HA) is a constitutive polysaccharide found in the extracellular matrix and is a material used in tissue engineering and scaffolding for tissue regeneration.
28109811	2	35	theme	constitutive	324:335	arg1	polysaccharide					337:350	a constitutive polysaccharide	322:350	a constitutive polysaccharide found in the extracellular matrix	322:384	Hyaluronic acid (HA) is a constitutive polysaccharide found in the extracellular matrix and is a material used in tissue engineering and scaffolding for tissue regeneration.
28109811	4	36	theme	molar	752:756	arg1	mass					758:761	molar mass	752:761	molar mass	752:761	The structural characteristics, thermal stability and molar mass of hyaluronic acid from chicken comb were evaluated.
28109811	4	37	theme	hyaluronic	766:775	arg1	acid					777:780	hyaluronic acid	766:780	hyaluronic acid from chicken comb	766:798	The structural characteristics, thermal stability and molar mass of hyaluronic acid from chicken comb were evaluated.
28109811	6	38	theme	low	1012:1014	arg1	mass					1022:1025	low molar mass hyaluronic acid	1012:1041	low molar mass hyaluronic acid with structural characteristics similar to the standard commercial hyaluronic acid	1012:1124	The optimized process of extraction and purification of hyaluronic acid resulted in low molar mass hyaluronic acid with structural characteristics similar to the standard commercial hyaluronic acid.
28109811	9	39	theme	tissue	1608:1613	arg1	engineering					1615:1625	tissue engineering	1608:1625	tissue engineering	1608:1625	The biocompatiblily of bacterial cellulose and the importance of hyaluronic acid as a component of extracellular matrix qualify the polymeric composites as promising biomaterials for tissue engineering.
28109811	9	40	theme	polymeric	1557:1565	arg1	composites					1567:1576	the polymeric composites	1553:1576	the polymeric composites	1553:1576	The biocompatiblily of bacterial cellulose and the importance of hyaluronic acid as a component of extracellular matrix qualify the polymeric composites as promising biomaterials for tissue engineering.
28109811	9	40	theme	polymeric	1557:1565	arg1	biomaterials					1591:1602	promising biomaterials	1581:1602	promising biomaterials for tissue engineering	1581:1625	The biocompatiblily of bacterial cellulose and the importance of hyaluronic acid as a component of extracellular matrix qualify the polymeric composites as promising biomaterials for tissue engineering.
28109811	3	41	theme	fermentation	601:612	arg1	days					593:596	different days	583:596	different days of fermentation	583:612	In this study, polymeric composites were produced in presence of hyaluronic acid isolated from chicken comb on different days of fermentation, specifically on the first (BCHA-SABT0) and third day (BCHA-SABT3) of fermentation.
28109811	1	42	theme	bacterial	109:117	arg1	BC					130:131	BC	130:131	BC	130:131	The bacterial cellulose (BC), from Gluconacetobacter hansenii, is a biofilm with a high degree of crystallinity that can be used for therapeutic purposes and as a candidate for healing wounds.
28109811	1	42	theme	bacterial	109:117	arg1	cellulose					119:127	The bacterial cellulose	105:127	The bacterial cellulose (BC)	105:132	The bacterial cellulose (BC), from Gluconacetobacter hansenii, is a biofilm with a high degree of crystallinity that can be used for therapeutic purposes and as a candidate for healing wounds.
28109811	1	42	theme	bacterial	109:117	arg1	biofilm					173:179	a biofilm	171:179	a biofilm with a high degree of crystallinity that can be used for therapeutic purposes and as a candidate for healing wounds	171:295	The bacterial cellulose (BC), from Gluconacetobacter hansenii, is a biofilm with a high degree of crystallinity that can be used for therapeutic purposes and as a candidate for healing wounds.
28109811	9	43	theme	matrix	1538:1543	arg1	component					1511:1519	a component	1509:1519	a component of extracellular matrix	1509:1543	The biocompatiblily of bacterial cellulose and the importance of hyaluronic acid as a component of extracellular matrix qualify the polymeric composites as promising biomaterials for tissue engineering.
28109811	6	44	theme	molar	1016:1020	arg1	mass					1022:1025	low molar mass hyaluronic acid	1012:1041	low molar mass hyaluronic acid with structural characteristics similar to the standard commercial hyaluronic acid	1012:1124	The optimized process of extraction and purification of hyaluronic acid resulted in low molar mass hyaluronic acid with structural characteristics similar to the standard commercial hyaluronic acid.
28109811	0	45	theme	cellulose	45:53	arg1	membranes					55:63	bacterial cellulose membranes	35:63	bacterial cellulose membranes	35:63	Production and characterization of bacterial cellulose membranes with hyaluronic acid from chicken comb.
28109811	9	46	theme	acid	1501:1504	arg1	biocompatiblily					1429:1443	The biocompatiblily	1425:1443	The biocompatiblily of bacterial cellulose	1425:1466	The biocompatiblily of bacterial cellulose and the importance of hyaluronic acid as a component of extracellular matrix qualify the polymeric composites as promising biomaterials for tissue engineering.
28109811	9	46	theme	acid	1501:1504	arg1	importance					1476:1485	the importance	1472:1485	the importance of hyaluronic acid as a component of extracellular matrix	1472:1543	The biocompatiblily of bacterial cellulose and the importance of hyaluronic acid as a component of extracellular matrix qualify the polymeric composites as promising biomaterials for tissue engineering.
28109811	1	47	theme	high	188:191	arg1	degree					193:198	a high degree	186:198	a high degree of crystallinity	186:215	The bacterial cellulose (BC), from Gluconacetobacter hansenii, is a biofilm with a high degree of crystallinity that can be used for therapeutic purposes and as a candidate for healing wounds.
28109811	8	48	theme	higher	1383:1388	arg1	roughness					1390:1398	higher roughness	1383:1398	higher roughness	1383:1398	The membranes produced on the third day presented better incorporation of HA-SAB between cellulose microfiber, resulting in membranes with higher thermal stability, higher roughness and lower crystallinity.
28109811	6	49	theme	hyaluronic	1027:1036	arg1	acid					1038:1041	hyaluronic acid	1027:1041	low molar mass hyaluronic acid with structural characteristics similar to the standard commercial hyaluronic acid	1012:1124	The optimized process of extraction and purification of hyaluronic acid resulted in low molar mass hyaluronic acid with structural characteristics similar to the standard commercial hyaluronic acid.
28109811	2	50	theme	extracellular	365:377	arg1	matrix					379:384	the extracellular matrix	361:384	the extracellular matrix	361:384	Hyaluronic acid (HA) is a constitutive polysaccharide found in the extracellular matrix and is a material used in tissue engineering and scaffolding for tissue regeneration.
28109811	0	51	theme	bacterial	35:43	arg1	membranes					55:63	bacterial cellulose membranes	35:63	bacterial cellulose membranes	35:63	Production and characterization of bacterial cellulose membranes with hyaluronic acid from chicken comb.
28109811	1	52	used	used	229:232	arg2	cellulose					119:127	The bacterial cellulose	105:127	The bacterial cellulose (BC)	105:132	The bacterial cellulose (BC), from Gluconacetobacter hansenii, is a biofilm with a high degree of crystallinity that can be used for therapeutic purposes and as a candidate for healing wounds.
28109811	1	52	used	used	229:232	arg2	biofilm					173:179	a biofilm	171:179	a biofilm with a high degree of crystallinity that can be used for therapeutic purposes and as a candidate for healing wounds	171:295	The bacterial cellulose (BC), from Gluconacetobacter hansenii, is a biofilm with a high degree of crystallinity that can be used for therapeutic purposes and as a candidate for healing wounds.
28109811	1	52	used	used	229:232	arg2	candidate					268:276	a candidate	266:276	a candidate for healing wounds	266:295	The bacterial cellulose (BC), from Gluconacetobacter hansenii, is a biofilm with a high degree of crystallinity that can be used for therapeutic purposes and as a candidate for healing wounds.
28109811	8	53	theme	third	1248:1252	arg1	day					1254:1256	the third day	1244:1256	the third day	1244:1256	The membranes produced on the third day presented better incorporation of HA-SAB between cellulose microfiber, resulting in membranes with higher thermal stability, higher roughness and lower crystallinity.
28109811	2	54	theme	tissue	412:417	arg1	engineering					419:429	tissue engineering	412:429	tissue engineering	412:429	Hyaluronic acid (HA) is a constitutive polysaccharide found in the extracellular matrix and is a material used in tissue engineering and scaffolding for tissue regeneration.
28109811	0	55	theme	hyaluronic	70:79	arg1	acid					81:84	hyaluronic acid	70:84	hyaluronic acid from chicken comb	70:102	Production and characterization of bacterial cellulose membranes with hyaluronic acid from chicken comb.
28109811	3	56	theme	hyaluronic	537:546	arg1	acid					548:551	hyaluronic acid	537:551	hyaluronic acid isolated from chicken comb on different days of fermentation, specifically on the first (BCHA-SABT0) and third day (BCHA-SABT3) of fermentation	537:695	In this study, polymeric composites were produced in presence of hyaluronic acid isolated from chicken comb on different days of fermentation, specifically on the first (BCHA-SABT0) and third day (BCHA-SABT3) of fermentation.
28109811	0	57	with	characterization	15:30	arg1	acid					81:84	hyaluronic acid	70:84	hyaluronic acid from chicken comb	70:102	Production and characterization of bacterial cellulose membranes with hyaluronic acid from chicken comb.
28109811	8	58	theme	better	1268:1273	arg1	incorporation					1275:1287	better incorporation	1268:1287	better incorporation of HA-SAB between cellulose microfiber	1268:1326	The membranes produced on the third day presented better incorporation of HA-SAB between cellulose microfiber, resulting in membranes with higher thermal stability, higher roughness and lower crystallinity.
28109811	0	59	theme	membranes	55:63	arg1	characterization					15:30	characterization	15:30	characterization	15:30	Production and characterization of bacterial cellulose membranes with hyaluronic acid from chicken comb.
28109811	0	59	theme	membranes	55:63	arg1	Production					0:9	Production	0:9	Production	0:9	Production and characterization of bacterial cellulose membranes with hyaluronic acid from chicken comb.
28109811	1	60	theme	healing	282:288	arg1	wounds					290:295	healing wounds	282:295	healing wounds	282:295	The bacterial cellulose (BC), from Gluconacetobacter hansenii, is a biofilm with a high degree of crystallinity that can be used for therapeutic purposes and as a candidate for healing wounds.
28109811	1	61	with	biofilm	173:179	arg1	degree					193:198	a high degree	186:198	a high degree of crystallinity	186:215	The bacterial cellulose (BC), from Gluconacetobacter hansenii, is a biofilm with a high degree of crystallinity that can be used for therapeutic purposes and as a candidate for healing wounds.
28109811	2	62	located	found	352:356	arg1	matrix					379:384	the extracellular matrix	361:384	the extracellular matrix	361:384	Hyaluronic acid (HA) is a constitutive polysaccharide found in the extracellular matrix and is a material used in tissue engineering and scaffolding for tissue regeneration.
28109811	2	62	located	found	352:356	arg2	polysaccharide					337:350	a constitutive polysaccharide	322:350	a constitutive polysaccharide found in the extracellular matrix	322:384	Hyaluronic acid (HA) is a constitutive polysaccharide found in the extracellular matrix and is a material used in tissue engineering and scaffolding for tissue regeneration.
28109811	2	62	located	found	352:356	arg2	acid					309:312	Hyaluronic acid	298:312	Hyaluronic acid (HA)	298:317	Hyaluronic acid (HA) is a constitutive polysaccharide found in the extracellular matrix and is a material used in tissue engineering and scaffolding for tissue regeneration.
28109811	3	63	theme	acid	548:551	arg1	presence					525:532	presence	525:532	presence of hyaluronic acid isolated from chicken comb on different days of fermentation, specifically on the first (BCHA-SABT0) and third day (BCHA-SABT3) of fermentation	525:695	In this study, polymeric composites were produced in presence of hyaluronic acid isolated from chicken comb on different days of fermentation, specifically on the first (BCHA-SABT0) and third day (BCHA-SABT3) of fermentation.
28109811	6	64	theme	hyaluronic	1110:1119	arg1	acid					1121:1124	the standard commercial hyaluronic acid	1086:1124	the standard commercial hyaluronic acid	1086:1124	The optimized process of extraction and purification of hyaluronic acid resulted in low molar mass hyaluronic acid with structural characteristics similar to the standard commercial hyaluronic acid.
28109811	3	65	theme	first	635:639	arg1	day					664:666	the first (BCHA-SABT0) and third day	631:666	the first (BCHA-SABT0) and third day (BCHA-SABT3) of fermentation	631:695	In this study, polymeric composites were produced in presence of hyaluronic acid isolated from chicken comb on different days of fermentation, specifically on the first (BCHA-SABT0) and third day (BCHA-SABT3) of fermentation.
28109811	3	65	theme	first	635:639	arg1	BCHA-SABT3					669:678	BCHA-SABT3	669:678	BCHA-SABT3	669:678	In this study, polymeric composites were produced in presence of hyaluronic acid isolated from chicken comb on different days of fermentation, specifically on the first (BCHA-SABT0) and third day (BCHA-SABT3) of fermentation.
28109811	0	66	with	Production	0:9	arg1	acid					81:84	hyaluronic acid	70:84	hyaluronic acid from chicken comb	70:102	Production and characterization of bacterial cellulose membranes with hyaluronic acid from chicken comb.
28109811	2	67	theme	Hyaluronic	298:307	arg1	acid					309:312	Hyaluronic acid	298:312	Hyaluronic acid (HA)	298:317	Hyaluronic acid (HA) is a constitutive polysaccharide found in the extracellular matrix and is a material used in tissue engineering and scaffolding for tissue regeneration.
28109811	2	67	theme	Hyaluronic	298:307	arg1	polysaccharide					337:350	a constitutive polysaccharide	322:350	a constitutive polysaccharide found in the extracellular matrix	322:384	Hyaluronic acid (HA) is a constitutive polysaccharide found in the extracellular matrix and is a material used in tissue engineering and scaffolding for tissue regeneration.
28109811	2	67	theme	Hyaluronic	298:307	arg1	HA					315:316	HA	315:316	HA	315:316	Hyaluronic acid (HA) is a constitutive polysaccharide found in the extracellular matrix and is a material used in tissue engineering and scaffolding for tissue regeneration.
28109811	8	68	with	membranes	1342:1350	arg1	roughness					1390:1398	higher roughness	1383:1398	higher roughness	1383:1398	The membranes produced on the third day presented better incorporation of HA-SAB between cellulose microfiber, resulting in membranes with higher thermal stability, higher roughness and lower crystallinity.
28109811	8	68	with	membranes	1342:1350	arg1	crystallinity					1410:1422	lower crystallinity	1404:1422	lower crystallinity	1404:1422	The membranes produced on the third day presented better incorporation of HA-SAB between cellulose microfiber, resulting in membranes with higher thermal stability, higher roughness and lower crystallinity.
28109811	8	68	with	membranes	1342:1350	arg1	stability					1372:1380	higher thermal stability	1357:1380	higher thermal stability	1357:1380	The membranes produced on the third day presented better incorporation of HA-SAB between cellulose microfiber, resulting in membranes with higher thermal stability, higher roughness and lower crystallinity.
28109811	4	69	from	mass	758:761	arg1	comb					795:798	chicken comb	787:798	chicken comb	787:798	The structural characteristics, thermal stability and molar mass of hyaluronic acid from chicken comb were evaluated.
28109811	6	70	with	mass	1022:1025	arg1	characteristics					1059:1073	structural characteristics	1048:1073	structural characteristics similar to the standard commercial hyaluronic acid	1048:1124	The optimized process of extraction and purification of hyaluronic acid resulted in low molar mass hyaluronic acid with structural characteristics similar to the standard commercial hyaluronic acid.
28109811	3	71	theme	different	583:591	arg1	days					593:596	different days	583:596	different days of fermentation	583:612	In this study, polymeric composites were produced in presence of hyaluronic acid isolated from chicken comb on different days of fermentation, specifically on the first (BCHA-SABT0) and third day (BCHA-SABT3) of fermentation.
28109811	6	72	theme	standard	1090:1097	arg1	acid					1121:1124	the standard commercial hyaluronic acid	1086:1124	the standard commercial hyaluronic acid	1086:1124	The optimized process of extraction and purification of hyaluronic acid resulted in low molar mass hyaluronic acid with structural characteristics similar to the standard commercial hyaluronic acid.
28109811	1	73	theme	crystallinity	203:215	arg1	degree					193:198	a high degree	186:198	a high degree of crystallinity	186:215	The bacterial cellulose (BC), from Gluconacetobacter hansenii, is a biofilm with a high degree of crystallinity that can be used for therapeutic purposes and as a candidate for healing wounds.
28109811	0	74	from	comb	99:102	arg1	acid					81:84	hyaluronic acid	70:84	hyaluronic acid from chicken comb	70:102	Production and characterization of bacterial cellulose membranes with hyaluronic acid from chicken comb.
28109811	3	75	theme	chicken	567:573	arg1	comb					575:578	chicken comb	567:578	chicken comb on different days of fermentation, specifically on the first (BCHA-SABT0) and third day (BCHA-SABT3) of fermentation	567:695	In this study, polymeric composites were produced in presence of hyaluronic acid isolated from chicken comb on different days of fermentation, specifically on the first (BCHA-SABT0) and third day (BCHA-SABT3) of fermentation.
28109811	6	76	theme	hyaluronic	984:993	arg1	acid					995:998	hyaluronic acid	984:998	hyaluronic acid	984:998	The optimized process of extraction and purification of hyaluronic acid resulted in low molar mass hyaluronic acid with structural characteristics similar to the standard commercial hyaluronic acid.
28109811	2	77	theme	tissue	451:456	arg1	regeneration					458:469	tissue regeneration	451:469	tissue regeneration	451:469	Hyaluronic acid (HA) is a constitutive polysaccharide found in the extracellular matrix and is a material used in tissue engineering and scaffolding for tissue regeneration.
28109811	9	78	theme	bacterial	1448:1456	arg1	cellulose					1458:1466	bacterial cellulose	1448:1466	bacterial cellulose	1448:1466	The biocompatiblily of bacterial cellulose and the importance of hyaluronic acid as a component of extracellular matrix qualify the polymeric composites as promising biomaterials for tissue engineering.
28109811	4	79	from	stability	738:746	arg1	comb					795:798	chicken comb	787:798	chicken comb	787:798	The structural characteristics, thermal stability and molar mass of hyaluronic acid from chicken comb were evaluated.
28109811	5	80	theme	polymeric	836:844	arg1	composites					846:855	polymeric composites	836:855	polymeric composites	836:855	Native membrane and polymeric composites were characterized with respect to their morphology and crystallinity.
26325384	0	0	theme	Biomolecules	95:106	arg1	Release					84:90	Temporally Controlled Release	62:90	Temporally Controlled Release of Biomolecules	62:106	Composite Scaffolds of Interfacial Polyelectrolyte Fibers for Temporally Controlled Release of Biomolecules.
26325384	1	1	theme	biochemical	175:185	arg1	environment					187:197	a controlled biochemical environment	162:197	a controlled biochemical environment to mimic the physiological cell niche	162:235	Various scaffolds used in tissue engineering require a controlled biochemical environment to mimic the physiological cell niche.
26325384	6	2	theme	cell	1070:1073	arg1	behavior					1075:1082	cell behavior	1070:1082	cell behavior	1070:1082	We also created a composite polymeric scaffold with topographical cues and sustained biochemical release that can have synergistic effects on cell behavior.
26325384	3	3	from	simplicity	427:436	arg1	fibers					471:476	making IPC fibers	460:476	making IPC fibers	460:476	The simplicity of the methodology in making IPC fibers gives flexibility in its application for controlled biomolecule delivery.
26325384	3	4	theme	biomolecule	530:540	arg1	delivery					542:549	controlled biomolecule delivery	519:549	controlled biomolecule delivery	519:549	The simplicity of the methodology in making IPC fibers gives flexibility in its application for controlled biomolecule delivery.
26325384	4	5	theme	IPC	596:598	arg1	fibers					600:605	IPC fibers	596:605	IPC fibers	596:605	Here, we describe a method of incorporating IPC fibers into two different polymeric scaffolds, hydrophilic polysaccharide and hydrophobic polycaprolactone, to create a multi-component composite scaffold.
26325384	5	6	theme	IPC	771:773	arg1	fibers					775:780	IPC fibers	771:780	IPC fibers	771:780	We showed that IPC fibers can be easily embedded into these polymeric structures, enhancing the capability for sustained release and improved preservation of biomolecules.
26325384	6	7	theme	polymeric	956:964	arg1	scaffold					966:973	a composite polymeric scaffold	944:973	a composite polymeric scaffold	944:973	We also created a composite polymeric scaffold with topographical cues and sustained biochemical release that can have synergistic effects on cell behavior.
26325384	6	8	theme	composite	946:954	arg1	scaffold					966:973	a composite polymeric scaffold	944:973	a composite polymeric scaffold	944:973	We also created a composite polymeric scaffold with topographical cues and sustained biochemical release that can have synergistic effects on cell behavior.
26325384	7	9	theme	cellular	1185:1192	arg1	niche					1194:1198	the cellular niche	1181:1198	the cellular niche	1181:1198	Composite polymeric scaffolds with IPC fibers represent a novel and simple method of recreating the cellular niche.
26325384	2	10	theme	controlled	308:317	arg1	delivery					319:326	controlled delivery	308:326	controlled delivery of various biological agents such as small molecule drugs, cells, proteins and growth factors	308:420	Interfacial polyelectrolyte complexation (IPC) fibers can be used for controlled delivery of various biological agents such as small molecule drugs, cells, proteins and growth factors.
26325384	5	11	theme	improved	889:896	arg1	preservation					898:909	improved preservation	889:909	improved preservation	889:909	We showed that IPC fibers can be easily embedded into these polymeric structures, enhancing the capability for sustained release and improved preservation of biomolecules.
26325384	5	12	theme	biomolecules	914:925	arg1	release					877:883	sustained release	867:883	sustained release	867:883	We showed that IPC fibers can be easily embedded into these polymeric structures, enhancing the capability for sustained release and improved preservation of biomolecules.
26325384	5	12	theme	biomolecules	914:925	arg1	preservation					898:909	improved preservation	889:909	improved preservation	889:909	We showed that IPC fibers can be easily embedded into these polymeric structures, enhancing the capability for sustained release and improved preservation of biomolecules.
26325384	7	13	with	scaffolds	1105:1113	arg1	fibers					1124:1129	IPC fibers	1120:1129	IPC fibers	1120:1129	Composite polymeric scaffolds with IPC fibers represent a novel and simple method of recreating the cellular niche.
26325384	6	14	theme	synergistic	1047:1057	arg1	effects					1059:1065	synergistic effects	1047:1065	synergistic effects	1047:1065	We also created a composite polymeric scaffold with topographical cues and sustained biochemical release that can have synergistic effects on cell behavior.
26325384	5	15	theme	polymeric	816:824	arg1	structures					826:835	these polymeric structures	810:835	these polymeric structures	810:835	We showed that IPC fibers can be easily embedded into these polymeric structures, enhancing the capability for sustained release and improved preservation of biomolecules.
26325384	2	16	theme	growth	407:412	arg1	factors					414:420	growth factors	407:420	growth factors	407:420	Interfacial polyelectrolyte complexation (IPC) fibers can be used for controlled delivery of various biological agents such as small molecule drugs, cells, proteins and growth factors.
26325384	7	17	theme	simple	1153:1158	arg1	method					1160:1165	a novel and simple method	1141:1165	a novel and simple method of recreating the cellular niche	1141:1198	Composite polymeric scaffolds with IPC fibers represent a novel and simple method of recreating the cellular niche.
26325384	2	18	theme	agents	350:355	arg1	delivery					319:326	controlled delivery	308:326	controlled delivery of various biological agents such as small molecule drugs, cells, proteins and growth factors	308:420	Interfacial polyelectrolyte complexation (IPC) fibers can be used for controlled delivery of various biological agents such as small molecule drugs, cells, proteins and growth factors.
26325384	0	19	theme	Composite	0:8	arg1	Scaffolds					10:18	Composite Scaffolds	0:18	Composite Scaffolds of Interfacial Polyelectrolyte Fibers for Temporally Controlled Release of Biomolecules.	0:107	Composite Scaffolds of Interfacial Polyelectrolyte Fibers for Temporally Controlled Release of Biomolecules.
26325384	2	20	theme	biological	339:348	arg1	proteins					394:401	proteins	394:401	proteins	394:401	Interfacial polyelectrolyte complexation (IPC) fibers can be used for controlled delivery of various biological agents such as small molecule drugs, cells, proteins and growth factors.
26325384	2	20	theme	biological	339:348	arg1	factors					414:420	growth factors	407:420	growth factors	407:420	Interfacial polyelectrolyte complexation (IPC) fibers can be used for controlled delivery of various biological agents such as small molecule drugs, cells, proteins and growth factors.
26325384	2	20	theme	biological	339:348	arg1	cells					387:391	cells	387:391	cells	387:391	Interfacial polyelectrolyte complexation (IPC) fibers can be used for controlled delivery of various biological agents such as small molecule drugs, cells, proteins and growth factors.
26325384	2	20	theme	biological	339:348	arg1	drugs					380:384	small molecule drugs	365:384	small molecule drugs	365:384	Interfacial polyelectrolyte complexation (IPC) fibers can be used for controlled delivery of various biological agents such as small molecule drugs, cells, proteins and growth factors.
26325384	2	20	theme	biological	339:348	arg1	agents					350:355	various biological agents	331:355	various biological agents such as small molecule drugs, cells, proteins and growth factors	331:420	Interfacial polyelectrolyte complexation (IPC) fibers can be used for controlled delivery of various biological agents such as small molecule drugs, cells, proteins and growth factors.
26325384	1	21	theme	physiological	212:224	arg1	niche					231:235	the physiological cell niche	208:235	the physiological cell niche	208:235	Various scaffolds used in tissue engineering require a controlled biochemical environment to mimic the physiological cell niche.
26325384	0	22	theme	Interfacial	23:33	arg1	Fibers					51:56	Interfacial Polyelectrolyte Fibers	23:56	Interfacial Polyelectrolyte Fibers	23:56	Composite Scaffolds of Interfacial Polyelectrolyte Fibers for Temporally Controlled Release of Biomolecules.
26325384	7	23	theme	novel	1143:1147	arg1	method					1160:1165	a novel and simple method	1141:1165	a novel and simple method of recreating the cellular niche	1141:1198	Composite polymeric scaffolds with IPC fibers represent a novel and simple method of recreating the cellular niche.
26325384	6	24	contain	have	1042:1045	arg1	cues					994:997	topographical cues	980:997	topographical cues	980:997	We also created a composite polymeric scaffold with topographical cues and sustained biochemical release that can have synergistic effects on cell behavior.
26325384	6	24	contain	have	1042:1045	arg1	release					1025:1031	sustained biochemical release	1003:1031	sustained biochemical release	1003:1031	We also created a composite polymeric scaffold with topographical cues and sustained biochemical release that can have synergistic effects on cell behavior.
26325384	6	24	contain	have	1042:1045	arg2	effects					1059:1065	synergistic effects	1047:1065	synergistic effects	1047:1065	We also created a composite polymeric scaffold with topographical cues and sustained biochemical release that can have synergistic effects on cell behavior.
26325384	2	25	theme	various	331:337	arg1	proteins					394:401	proteins	394:401	proteins	394:401	Interfacial polyelectrolyte complexation (IPC) fibers can be used for controlled delivery of various biological agents such as small molecule drugs, cells, proteins and growth factors.
26325384	2	25	theme	various	331:337	arg1	factors					414:420	growth factors	407:420	growth factors	407:420	Interfacial polyelectrolyte complexation (IPC) fibers can be used for controlled delivery of various biological agents such as small molecule drugs, cells, proteins and growth factors.
26325384	2	25	theme	various	331:337	arg1	cells					387:391	cells	387:391	cells	387:391	Interfacial polyelectrolyte complexation (IPC) fibers can be used for controlled delivery of various biological agents such as small molecule drugs, cells, proteins and growth factors.
26325384	2	25	theme	various	331:337	arg1	drugs					380:384	small molecule drugs	365:384	small molecule drugs	365:384	Interfacial polyelectrolyte complexation (IPC) fibers can be used for controlled delivery of various biological agents such as small molecule drugs, cells, proteins and growth factors.
26325384	2	25	theme	various	331:337	arg1	agents					350:355	various biological agents	331:355	various biological agents such as small molecule drugs, cells, proteins and growth factors	331:420	Interfacial polyelectrolyte complexation (IPC) fibers can be used for controlled delivery of various biological agents such as small molecule drugs, cells, proteins and growth factors.
26325384	1	26	theme	cell	226:229	arg1	niche					231:235	the physiological cell niche	208:235	the physiological cell niche	208:235	Various scaffolds used in tissue engineering require a controlled biochemical environment to mimic the physiological cell niche.
26325384	1	27	theme	Various	109:115	arg1	scaffolds					117:125	Various scaffolds	109:125	Various scaffolds used in tissue engineering	109:152	Various scaffolds used in tissue engineering require a controlled biochemical environment to mimic the physiological cell niche.
26325384	4	28	theme	composite	736:744	arg1	scaffold					746:753	a multi-component composite scaffold	718:753	a multi-component composite scaffold	718:753	Here, we describe a method of incorporating IPC fibers into two different polymeric scaffolds, hydrophilic polysaccharide and hydrophobic polycaprolactone, to create a multi-component composite scaffold.
26325384	4	29	theme	hydrophilic	647:657	arg1	scaffolds					636:644	two different polymeric scaffolds	612:644	two different polymeric scaffolds	612:644	Here, we describe a method of incorporating IPC fibers into two different polymeric scaffolds, hydrophilic polysaccharide and hydrophobic polycaprolactone, to create a multi-component composite scaffold.
26325384	4	29	theme	hydrophilic	647:657	arg1	polysaccharide					659:672	hydrophilic polysaccharide	647:672	hydrophilic polysaccharide	647:672	Here, we describe a method of incorporating IPC fibers into two different polymeric scaffolds, hydrophilic polysaccharide and hydrophobic polycaprolactone, to create a multi-component composite scaffold.
26325384	0	30	theme	Fibers	51:56	arg1	Scaffolds					10:18	Composite Scaffolds	0:18	Composite Scaffolds of Interfacial Polyelectrolyte Fibers for Temporally Controlled Release of Biomolecules.	0:107	Composite Scaffolds of Interfacial Polyelectrolyte Fibers for Temporally Controlled Release of Biomolecules.
26325384	2	31	theme	small	365:369	arg1	drugs					380:384	small molecule drugs	365:384	small molecule drugs	365:384	Interfacial polyelectrolyte complexation (IPC) fibers can be used for controlled delivery of various biological agents such as small molecule drugs, cells, proteins and growth factors.
26325384	4	32	theme	hydrophobic	678:688	arg1	scaffolds					636:644	two different polymeric scaffolds	612:644	two different polymeric scaffolds	612:644	Here, we describe a method of incorporating IPC fibers into two different polymeric scaffolds, hydrophilic polysaccharide and hydrophobic polycaprolactone, to create a multi-component composite scaffold.
26325384	4	32	theme	hydrophobic	678:688	arg1	polycaprolactone					690:705	hydrophobic polycaprolactone	678:705	hydrophobic polycaprolactone	678:705	Here, we describe a method of incorporating IPC fibers into two different polymeric scaffolds, hydrophilic polysaccharide and hydrophobic polycaprolactone, to create a multi-component composite scaffold.
26325384	3	33	theme	methodology	445:455	arg1	simplicity					427:436	The simplicity	423:436	The simplicity of the methodology in making IPC fibers	423:476	The simplicity of the methodology in making IPC fibers gives flexibility in its application for controlled biomolecule delivery.
26325384	3	34	theme	IPC	467:469	arg1	fibers					471:476	making IPC fibers	460:476	making IPC fibers	460:476	The simplicity of the methodology in making IPC fibers gives flexibility in its application for controlled biomolecule delivery.
26325384	0	35	theme	Polyelectrolyte	35:49	arg1	Fibers					51:56	Interfacial Polyelectrolyte Fibers	23:56	Interfacial Polyelectrolyte Fibers	23:56	Composite Scaffolds of Interfacial Polyelectrolyte Fibers for Temporally Controlled Release of Biomolecules.
26325384	6	36	theme	biochemical	1013:1023	arg1	release					1025:1031	sustained biochemical release	1003:1031	sustained biochemical release	1003:1031	We also created a composite polymeric scaffold with topographical cues and sustained biochemical release that can have synergistic effects on cell behavior.
26325384	7	37	theme	Composite	1085:1093	arg1	scaffolds					1105:1113	Composite polymeric scaffolds	1085:1113	Composite polymeric scaffolds with IPC fibers	1085:1129	Composite polymeric scaffolds with IPC fibers represent a novel and simple method of recreating the cellular niche.
26325384	3	38	theme	controlled	519:528	arg1	delivery					542:549	controlled biomolecule delivery	519:549	controlled biomolecule delivery	519:549	The simplicity of the methodology in making IPC fibers gives flexibility in its application for controlled biomolecule delivery.
26325384	7	39	theme	IPC	1120:1122	arg1	fibers					1124:1129	IPC fibers	1120:1129	IPC fibers	1120:1129	Composite polymeric scaffolds with IPC fibers represent a novel and simple method of recreating the cellular niche.
26325384	2	40	theme	complexation	266:277	arg1	fibers					285:290	Interfacial polyelectrolyte complexation (IPC) fibers	238:290	Interfacial polyelectrolyte complexation (IPC) fibers	238:290	Interfacial polyelectrolyte complexation (IPC) fibers can be used for controlled delivery of various biological agents such as small molecule drugs, cells, proteins and growth factors.
26325384	7	41	theme	polymeric	1095:1103	arg1	scaffolds					1105:1113	Composite polymeric scaffolds	1085:1113	Composite polymeric scaffolds with IPC fibers	1085:1129	Composite polymeric scaffolds with IPC fibers represent a novel and simple method of recreating the cellular niche.
26325384	2	42	theme	molecule	371:378	arg1	drugs					380:384	small molecule drugs	365:384	small molecule drugs	365:384	Interfacial polyelectrolyte complexation (IPC) fibers can be used for controlled delivery of various biological agents such as small molecule drugs, cells, proteins and growth factors.
26325384	2	43	theme	Interfacial	238:248	arg1	IPC					280:282	IPC	280:282	IPC	280:282	Interfacial polyelectrolyte complexation (IPC) fibers can be used for controlled delivery of various biological agents such as small molecule drugs, cells, proteins and growth factors.
26325384	2	43	theme	Interfacial	238:248	arg1	complexation					266:277	Interfacial polyelectrolyte complexation	238:277	Interfacial polyelectrolyte complexation (IPC) fibers	238:290	Interfacial polyelectrolyte complexation (IPC) fibers can be used for controlled delivery of various biological agents such as small molecule drugs, cells, proteins and growth factors.
26325384	2	44	theme	polyelectrolyte	250:264	arg1	IPC					280:282	IPC	280:282	IPC	280:282	Interfacial polyelectrolyte complexation (IPC) fibers can be used for controlled delivery of various biological agents such as small molecule drugs, cells, proteins and growth factors.
26325384	2	44	theme	polyelectrolyte	250:264	arg1	complexation					266:277	Interfacial polyelectrolyte complexation	238:277	Interfacial polyelectrolyte complexation (IPC) fibers	238:290	Interfacial polyelectrolyte complexation (IPC) fibers can be used for controlled delivery of various biological agents such as small molecule drugs, cells, proteins and growth factors.
26325384	1	45	theme	tissue	135:140	arg1	engineering					142:152	tissue engineering	135:152	tissue engineering	135:152	Various scaffolds used in tissue engineering require a controlled biochemical environment to mimic the physiological cell niche.
26325384	4	46	theme	polymeric	626:634	arg1	scaffolds					636:644	two different polymeric scaffolds	612:644	two different polymeric scaffolds	612:644	Here, we describe a method of incorporating IPC fibers into two different polymeric scaffolds, hydrophilic polysaccharide and hydrophobic polycaprolactone, to create a multi-component composite scaffold.
26325384	4	46	theme	polymeric	626:634	arg1	polysaccharide					659:672	hydrophilic polysaccharide	647:672	hydrophilic polysaccharide	647:672	Here, we describe a method of incorporating IPC fibers into two different polymeric scaffolds, hydrophilic polysaccharide and hydrophobic polycaprolactone, to create a multi-component composite scaffold.
26325384	4	46	theme	polymeric	626:634	arg1	polycaprolactone					690:705	hydrophobic polycaprolactone	678:705	hydrophobic polycaprolactone	678:705	Here, we describe a method of incorporating IPC fibers into two different polymeric scaffolds, hydrophilic polysaccharide and hydrophobic polycaprolactone, to create a multi-component composite scaffold.
26325384	4	47	theme	different	616:624	arg1	scaffolds					636:644	two different polymeric scaffolds	612:644	two different polymeric scaffolds	612:644	Here, we describe a method of incorporating IPC fibers into two different polymeric scaffolds, hydrophilic polysaccharide and hydrophobic polycaprolactone, to create a multi-component composite scaffold.
26325384	4	47	theme	different	616:624	arg1	polysaccharide					659:672	hydrophilic polysaccharide	647:672	hydrophilic polysaccharide	647:672	Here, we describe a method of incorporating IPC fibers into two different polymeric scaffolds, hydrophilic polysaccharide and hydrophobic polycaprolactone, to create a multi-component composite scaffold.
26325384	4	47	theme	different	616:624	arg1	polycaprolactone					690:705	hydrophobic polycaprolactone	678:705	hydrophobic polycaprolactone	678:705	Here, we describe a method of incorporating IPC fibers into two different polymeric scaffolds, hydrophilic polysaccharide and hydrophobic polycaprolactone, to create a multi-component composite scaffold.
26325384	4	48	theme	multi-component	720:734	arg1	scaffold					746:753	a multi-component composite scaffold	718:753	a multi-component composite scaffold	718:753	Here, we describe a method of incorporating IPC fibers into two different polymeric scaffolds, hydrophilic polysaccharide and hydrophobic polycaprolactone, to create a multi-component composite scaffold.
26325384	3	49	theme	making	460:465	arg1	fibers					471:476	making IPC fibers	460:476	making IPC fibers	460:476	The simplicity of the methodology in making IPC fibers gives flexibility in its application for controlled biomolecule delivery.
26325384	0	50	theme	Controlled	73:82	arg1	Release					84:90	Temporally Controlled Release	62:90	Temporally Controlled Release of Biomolecules	62:106	Composite Scaffolds of Interfacial Polyelectrolyte Fibers for Temporally Controlled Release of Biomolecules.
26325384	6	51	theme	topographical	980:992	arg1	cues					994:997	topographical cues	980:997	topographical cues	980:997	We also created a composite polymeric scaffold with topographical cues and sustained biochemical release that can have synergistic effects on cell behavior.
26325384	5	52	theme	sustained	867:875	arg1	release					877:883	sustained release	867:883	sustained release	867:883	We showed that IPC fibers can be easily embedded into these polymeric structures, enhancing the capability for sustained release and improved preservation of biomolecules.
26325384	1	53	theme	controlled	164:173	arg1	environment					187:197	a controlled biochemical environment	162:197	a controlled biochemical environment to mimic the physiological cell niche	162:235	Various scaffolds used in tissue engineering require a controlled biochemical environment to mimic the physiological cell niche.
26325384	2	54	used	used	299:302	arg2	fibers					285:290	Interfacial polyelectrolyte complexation (IPC) fibers	238:290	Interfacial polyelectrolyte complexation (IPC) fibers	238:290	Interfacial polyelectrolyte complexation (IPC) fibers can be used for controlled delivery of various biological agents such as small molecule drugs, cells, proteins and growth factors.
26325384	6	55	theme	sustained	1003:1011	arg1	release					1025:1031	sustained biochemical release	1003:1031	sustained biochemical release	1003:1031	We also created a composite polymeric scaffold with topographical cues and sustained biochemical release that can have synergistic effects on cell behavior.
27716641	2	0	theme	low	462:464	arg1	hydrogels					496:504	low concentrated, soft biopolymer hydrogels	462:504	low concentrated, soft biopolymer hydrogels as core	462:512	We now demonstrate that combining high concentrated (16.7 wt%) alginate hydrogels as shell material with low concentrated, soft biopolymer hydrogels as core leads to mechanically stable and robust 3D scaffolds.
27716641	0	1	from	method	12:17	arg1	fashion					71:77	a core/shell fashion	58:77	a core/shell fashion by 3D plotting to achieve mechanically robust constructs	58:134	A versatile method for combining different biopolymers in a core/shell fashion by 3D plotting to achieve mechanically robust constructs.
27716641	4	2	theme	mechanical	866:875	arg1	properties					877:886	mechanical properties	866:886	mechanical properties	866:886	The respective c/s scaffolds were characterized concerning their morphology, mechanical properties and swelling behavior.
27716641	1	3	from	biomaterials	182:193	arg1	fashion					217:223	a core/shell (c/s) fashion	198:223	a core/shell (c/s) fashion	198:223	Three-dimensional extrusion of two different biomaterials in a core/shell (c/s) fashion has gained much interest in the last couple of years as it allows for fabricating constructs with novel and interesting properties.
27716641	8	4	theme	live	1284:1287	arg1	cells					1307:1311	live human endothelial cells	1284:1311	live human endothelial cells	1284:1311	Finally, live human endothelial cells were integrated in the core material, demonstrating that this new strategy can be used for bioprinting purposes as well.
27716641	7	5	theme	different	1190:1198	arg1	BMP-2					1225:1229	BMP-2	1225:1229	BMP-2	1225:1229	When core and shell were loaded with different growth factors (VEGF and BMP-2, respectively) a dual release was achieved.
27716641	7	5	theme	different	1190:1198	arg1	VEGF					1216:1219	VEGF	1216:1219	VEGF	1216:1219	When core and shell were loaded with different growth factors (VEGF and BMP-2, respectively) a dual release was achieved.
27716641	7	5	theme	different	1190:1198	arg1	factors					1207:1213	different growth factors	1190:1213	different growth factors (VEGF and BMP-2, respectively)	1190:1244	When core and shell were loaded with different growth factors (VEGF and BMP-2, respectively) a dual release was achieved.
27716641	1	6	theme	novel	323:327	arg1	properties					345:354	novel and interesting properties	323:354	novel and interesting properties	323:354	Three-dimensional extrusion of two different biomaterials in a core/shell (c/s) fashion has gained much interest in the last couple of years as it allows for fabricating constructs with novel and interesting properties.
27716641	3	7	theme	gelatin	600:606	arg1	materials-hydrogels					666:684	core materials-hydrogels	661:684	core materials-hydrogels which are too soft for 3D plotting of open-porous structures without an additional mechanical support	661:786	Alginate, chitosan, gellan gum, gelatin and collagen hydrogels were utilized successfully as core materials-hydrogels which are too soft for 3D plotting of open-porous structures without an additional mechanical support.
27716641	3	7	theme	gelatin	600:606	arg1	hydrogels					621:629	Alginate, chitosan, gellan gum, gelatin and collagen hydrogels	568:629	Alginate, chitosan, gellan gum, gelatin and collagen hydrogels	568:629	Alginate, chitosan, gellan gum, gelatin and collagen hydrogels were utilized successfully as core materials-hydrogels which are too soft for 3D plotting of open-porous structures without an additional mechanical support.
27716641	1	8	with	constructs	307:316	arg1	properties					345:354	novel and interesting properties	323:354	novel and interesting properties	323:354	Three-dimensional extrusion of two different biomaterials in a core/shell (c/s) fashion has gained much interest in the last couple of years as it allows for fabricating constructs with novel and interesting properties.
27716641	3	9	theme	core	661:664	arg1	materials-hydrogels					666:684	core materials-hydrogels	661:684	core materials-hydrogels which are too soft for 3D plotting of open-porous structures without an additional mechanical support	661:786	Alginate, chitosan, gellan gum, gelatin and collagen hydrogels were utilized successfully as core materials-hydrogels which are too soft for 3D plotting of open-porous structures without an additional mechanical support.
27716641	3	9	theme	core	661:664	arg1	hydrogels					621:629	Alginate, chitosan, gellan gum, gelatin and collagen hydrogels	568:629	Alginate, chitosan, gellan gum, gelatin and collagen hydrogels	568:629	Alginate, chitosan, gellan gum, gelatin and collagen hydrogels were utilized successfully as core materials-hydrogels which are too soft for 3D plotting of open-porous structures without an additional mechanical support.
27716641	8	10	theme	endothelial	1295:1305	arg1	cells					1307:1311	live human endothelial cells	1284:1311	live human endothelial cells	1284:1311	Finally, live human endothelial cells were integrated in the core material, demonstrating that this new strategy can be used for bioprinting purposes as well.
27716641	8	11	theme	new	1375:1377	arg1	well					1428:1431	well	1428:1431	well	1428:1431	Finally, live human endothelial cells were integrated in the core material, demonstrating that this new strategy can be used for bioprinting purposes as well.
27716641	8	11	theme	new	1375:1377	arg1	strategy					1379:1386	this new strategy	1370:1386	this new strategy	1370:1386	Finally, live human endothelial cells were integrated in the core material, demonstrating that this new strategy can be used for bioprinting purposes as well.
27716641	5	12	theme	shell	950:954	arg1	part					956:959	shell part	950:959	core as well as shell part	934:959	It could be shown that core as well as shell part can be loaded with growth factors and that the release depends on core composition and shell thickness.
27716641	0	13	theme	3D	82:83	arg1	plotting					85:92	3D plotting	82:92	3D plotting	82:92	A versatile method for combining different biopolymers in a core/shell fashion by 3D plotting to achieve mechanically robust constructs.
27716641	1	14	theme	interesting	333:343	arg1	properties					345:354	novel and interesting properties	323:354	novel and interesting properties	323:354	Three-dimensional extrusion of two different biomaterials in a core/shell (c/s) fashion has gained much interest in the last couple of years as it allows for fabricating constructs with novel and interesting properties.
27716641	8	15	theme	human	1289:1293	arg1	cells					1307:1311	live human endothelial cells	1284:1311	live human endothelial cells	1284:1311	Finally, live human endothelial cells were integrated in the core material, demonstrating that this new strategy can be used for bioprinting purposes as well.
27716641	2	16	theme	biopolymer	485:494	arg1	hydrogels					496:504	low concentrated, soft biopolymer hydrogels	462:504	low concentrated, soft biopolymer hydrogels as core	462:512	We now demonstrate that combining high concentrated (16.7 wt%) alginate hydrogels as shell material with low concentrated, soft biopolymer hydrogels as core leads to mechanically stable and robust 3D scaffolds.
27716641	1	17	from	extrusion	155:163	arg1	fashion					217:223	a core/shell (c/s) fashion	198:223	a core/shell (c/s) fashion	198:223	Three-dimensional extrusion of two different biomaterials in a core/shell (c/s) fashion has gained much interest in the last couple of years as it allows for fabricating constructs with novel and interesting properties.
27716641	1	18	theme	different	172:180	arg1	biomaterials					182:193	two different biomaterials	168:193	two different biomaterials in a core/shell (c/s) fashion	168:223	Three-dimensional extrusion of two different biomaterials in a core/shell (c/s) fashion has gained much interest in the last couple of years as it allows for fabricating constructs with novel and interesting properties.
27716641	1	19	theme	biomaterials	182:193	arg1	extrusion					155:163	Three-dimensional extrusion	137:163	Three-dimensional extrusion of two different biomaterials in a core/shell (c/s) fashion	137:223	Three-dimensional extrusion of two different biomaterials in a core/shell (c/s) fashion has gained much interest in the last couple of years as it allows for fabricating constructs with novel and interesting properties.
27716641	0	20	theme	versatile	2:10	arg1	method					12:17	A versatile method	0:17	A versatile method for combining different biopolymers in a core/shell fashion by 3D plotting to achieve mechanically robust constructs.	0:135	A versatile method for combining different biopolymers in a core/shell fashion by 3D plotting to achieve mechanically robust constructs.
27716641	7	21	theme	dual	1248:1251	arg1	release					1253:1259	a dual release	1246:1259	a dual release	1246:1259	When core and shell were loaded with different growth factors (VEGF and BMP-2, respectively) a dual release was achieved.
27716641	5	22	theme	growth	980:985	arg1	factors					987:993	growth factors	980:993	growth factors	980:993	It could be shown that core as well as shell part can be loaded with growth factors and that the release depends on core composition and shell thickness.
27716641	4	23	theme	swelling	892:899	arg1	behavior					901:908	swelling behavior	892:908	swelling behavior	892:908	The respective c/s scaffolds were characterized concerning their morphology, mechanical properties and swelling behavior.
27716641	7	24	dep	factors	1207:1213	arg1	BMP-2					1225:1229	BMP-2	1225:1229	BMP-2	1225:1229	When core and shell were loaded with different growth factors (VEGF and BMP-2, respectively) a dual release was achieved.
27716641	7	24	dep	factors	1207:1213	arg1	VEGF					1216:1219	VEGF	1216:1219	VEGF	1216:1219	When core and shell were loaded with different growth factors (VEGF and BMP-2, respectively) a dual release was achieved.
27716641	7	24	dep	factors	1207:1213	arg1	factors					1207:1213	different growth factors	1190:1213	different growth factors (VEGF and BMP-2, respectively)	1190:1244	When core and shell were loaded with different growth factors (VEGF and BMP-2, respectively) a dual release was achieved.
27716641	5	25	theme	shell	1048:1052	arg1	thickness					1054:1062	shell thickness	1048:1062	shell thickness	1048:1062	It could be shown that core as well as shell part can be loaded with growth factors and that the release depends on core composition and shell thickness.
27716641	3	26	theme	chitosan	578:585	arg1	materials-hydrogels					666:684	core materials-hydrogels	661:684	core materials-hydrogels which are too soft for 3D plotting of open-porous structures without an additional mechanical support	661:786	Alginate, chitosan, gellan gum, gelatin and collagen hydrogels were utilized successfully as core materials-hydrogels which are too soft for 3D plotting of open-porous structures without an additional mechanical support.
27716641	3	26	theme	chitosan	578:585	arg1	hydrogels					621:629	Alginate, chitosan, gellan gum, gelatin and collagen hydrogels	568:629	Alginate, chitosan, gellan gum, gelatin and collagen hydrogels	568:629	Alginate, chitosan, gellan gum, gelatin and collagen hydrogels were utilized successfully as core materials-hydrogels which are too soft for 3D plotting of open-porous structures without an additional mechanical support.
27716641	0	27	theme	robust	118:123	arg1	constructs					125:134	mechanically robust constructs	105:134	mechanically robust constructs	105:134	A versatile method for combining different biopolymers in a core/shell fashion by 3D plotting to achieve mechanically robust constructs.
27716641	8	28	theme	core	1336:1339	arg1	material					1341:1348	the core material	1332:1348	the core material	1332:1348	Finally, live human endothelial cells were integrated in the core material, demonstrating that this new strategy can be used for bioprinting purposes as well.
27716641	4	29	theme	c/s	804:806	arg1	scaffolds					808:816	The respective c/s scaffolds	789:816	The respective c/s scaffolds	789:816	The respective c/s scaffolds were characterized concerning their morphology, mechanical properties and swelling behavior.
27716641	1	30	theme	core/shell	200:209	arg1	fashion					217:223	a core/shell (c/s) fashion	198:223	a core/shell (c/s) fashion	198:223	Three-dimensional extrusion of two different biomaterials in a core/shell (c/s) fashion has gained much interest in the last couple of years as it allows for fabricating constructs with novel and interesting properties.
27716641	2	31	theme	wt	415:416	arg1	%					417:417	16.7 wt%	410:417	16.7 wt%	410:417	We now demonstrate that combining high concentrated (16.7 wt%) alginate hydrogels as shell material with low concentrated, soft biopolymer hydrogels as core leads to mechanically stable and robust 3D scaffolds.
27716641	2	31	theme	wt	415:416	arg1	hydrogels					429:437	high concentrated (16.7 wt%) alginate hydrogels	391:437	high concentrated (16.7 wt%) alginate hydrogels as shell material	391:455	We now demonstrate that combining high concentrated (16.7 wt%) alginate hydrogels as shell material with low concentrated, soft biopolymer hydrogels as core leads to mechanically stable and robust 3D scaffolds.
27716641	1	32	theme	years	272:276	arg1	last					257:260	last	257:260	last	257:260	Three-dimensional extrusion of two different biomaterials in a core/shell (c/s) fashion has gained much interest in the last couple of years as it allows for fabricating constructs with novel and interesting properties.
27716641	0	33	theme	mechanically	105:116	arg1	constructs					125:134	mechanically robust constructs	105:134	mechanically robust constructs	105:134	A versatile method for combining different biopolymers in a core/shell fashion by 3D plotting to achieve mechanically robust constructs.
27716641	6	34	theme	1M	1120:1121	arg1	CaCl2					1123:1127	1M CaCl2	1120:1127	1M CaCl2	1120:1127	Neither the plotting process nor the crosslinking with 1M CaCl2 denatured the proteins.
27716641	0	35	theme	different	33:41	arg1	biopolymers					43:53	combining different biopolymers	23:53	combining different biopolymers	23:53	A versatile method for combining different biopolymers in a core/shell fashion by 3D plotting to achieve mechanically robust constructs.
27716641	8	36	used	used	1395:1398	arg2	strategy					1379:1386	this new strategy	1370:1386	this new strategy	1370:1386	Finally, live human endothelial cells were integrated in the core material, demonstrating that this new strategy can be used for bioprinting purposes as well.
27716641	8	36	used	used	1395:1398	arg2	well					1428:1431	well	1428:1431	well	1428:1431	Finally, live human endothelial cells were integrated in the core material, demonstrating that this new strategy can be used for bioprinting purposes as well.
27716641	3	37	theme	collagen	612:619	arg1	materials-hydrogels					666:684	core materials-hydrogels	661:684	core materials-hydrogels which are too soft for 3D plotting of open-porous structures without an additional mechanical support	661:786	Alginate, chitosan, gellan gum, gelatin and collagen hydrogels were utilized successfully as core materials-hydrogels which are too soft for 3D plotting of open-porous structures without an additional mechanical support.
27716641	3	37	theme	collagen	612:619	arg1	hydrogels					621:629	Alginate, chitosan, gellan gum, gelatin and collagen hydrogels	568:629	Alginate, chitosan, gellan gum, gelatin and collagen hydrogels	568:629	Alginate, chitosan, gellan gum, gelatin and collagen hydrogels were utilized successfully as core materials-hydrogels which are too soft for 3D plotting of open-porous structures without an additional mechanical support.
27716641	3	38	theme	Alginate	568:575	arg1	materials-hydrogels					666:684	core materials-hydrogels	661:684	core materials-hydrogels which are too soft for 3D plotting of open-porous structures without an additional mechanical support	661:786	Alginate, chitosan, gellan gum, gelatin and collagen hydrogels were utilized successfully as core materials-hydrogels which are too soft for 3D plotting of open-porous structures without an additional mechanical support.
27716641	3	38	theme	Alginate	568:575	arg1	hydrogels					621:629	Alginate, chitosan, gellan gum, gelatin and collagen hydrogels	568:629	Alginate, chitosan, gellan gum, gelatin and collagen hydrogels	568:629	Alginate, chitosan, gellan gum, gelatin and collagen hydrogels were utilized successfully as core materials-hydrogels which are too soft for 3D plotting of open-porous structures without an additional mechanical support.
27716641	2	39	theme	concentrated	396:407	arg1	%					417:417	16.7 wt%	410:417	16.7 wt%	410:417	We now demonstrate that combining high concentrated (16.7 wt%) alginate hydrogels as shell material with low concentrated, soft biopolymer hydrogels as core leads to mechanically stable and robust 3D scaffolds.
27716641	2	39	theme	concentrated	396:407	arg1	hydrogels					429:437	high concentrated (16.7 wt%) alginate hydrogels	391:437	high concentrated (16.7 wt%) alginate hydrogels as shell material	391:455	We now demonstrate that combining high concentrated (16.7 wt%) alginate hydrogels as shell material with low concentrated, soft biopolymer hydrogels as core leads to mechanically stable and robust 3D scaffolds.
27716641	0	40	theme	combining	23:31	arg1	biopolymers					43:53	combining different biopolymers	23:53	combining different biopolymers	23:53	A versatile method for combining different biopolymers in a core/shell fashion by 3D plotting to achieve mechanically robust constructs.
27716641	2	41	theme	shell	442:446	arg1	material					448:455	shell material	442:455	shell material	442:455	We now demonstrate that combining high concentrated (16.7 wt%) alginate hydrogels as shell material with low concentrated, soft biopolymer hydrogels as core leads to mechanically stable and robust 3D scaffolds.
27716641	8	42	theme	bioprinting	1404:1414	arg1	purposes					1416:1423	bioprinting purposes	1404:1423	bioprinting purposes	1404:1423	Finally, live human endothelial cells were integrated in the core material, demonstrating that this new strategy can be used for bioprinting purposes as well.
27716641	3	43	theme	mechanical	769:778	arg1	support					780:786	an additional mechanical support	755:786	an additional mechanical support	755:786	Alginate, chitosan, gellan gum, gelatin and collagen hydrogels were utilized successfully as core materials-hydrogels which are too soft for 3D plotting of open-porous structures without an additional mechanical support.
27716641	2	44	theme	high	391:394	arg1	%					417:417	16.7 wt%	410:417	16.7 wt%	410:417	We now demonstrate that combining high concentrated (16.7 wt%) alginate hydrogels as shell material with low concentrated, soft biopolymer hydrogels as core leads to mechanically stable and robust 3D scaffolds.
27716641	2	44	theme	high	391:394	arg1	hydrogels					429:437	high concentrated (16.7 wt%) alginate hydrogels	391:437	high concentrated (16.7 wt%) alginate hydrogels as shell material	391:455	We now demonstrate that combining high concentrated (16.7 wt%) alginate hydrogels as shell material with low concentrated, soft biopolymer hydrogels as core leads to mechanically stable and robust 3D scaffolds.
27716641	3	45	theme	additional	758:767	arg1	support					780:786	an additional mechanical support	755:786	an additional mechanical support	755:786	Alginate, chitosan, gellan gum, gelatin and collagen hydrogels were utilized successfully as core materials-hydrogels which are too soft for 3D plotting of open-porous structures without an additional mechanical support.
27716641	2	46	theme	3D	554:555	arg1	scaffolds					557:565	mechanically stable and robust 3D scaffolds	523:565	mechanically stable and robust 3D scaffolds	523:565	We now demonstrate that combining high concentrated (16.7 wt%) alginate hydrogels as shell material with low concentrated, soft biopolymer hydrogels as core leads to mechanically stable and robust 3D scaffolds.
27716641	2	47	dep	concentrated	466:477	arg1	soft					480:483	soft	480:483	soft	480:483	We now demonstrate that combining high concentrated (16.7 wt%) alginate hydrogels as shell material with low concentrated, soft biopolymer hydrogels as core leads to mechanically stable and robust 3D scaffolds.
27716641	1	48	contain	has	225:227	arg1	extrusion					155:163	Three-dimensional extrusion	137:163	Three-dimensional extrusion of two different biomaterials in a core/shell (c/s) fashion	137:223	Three-dimensional extrusion of two different biomaterials in a core/shell (c/s) fashion has gained much interest in the last couple of years as it allows for fabricating constructs with novel and interesting properties.
27716641	1	48	contain	has	225:227	arg2	interest					241:248	gained much interest	229:248	gained much interest	229:248	Three-dimensional extrusion of two different biomaterials in a core/shell (c/s) fashion has gained much interest in the last couple of years as it allows for fabricating constructs with novel and interesting properties.
27716641	3	49	theme	open-porous	724:734	arg1	structures					736:745	open-porous structures	724:745	open-porous structures	724:745	Alginate, chitosan, gellan gum, gelatin and collagen hydrogels were utilized successfully as core materials-hydrogels which are too soft for 3D plotting of open-porous structures without an additional mechanical support.
27716641	6	50	with	crosslinking	1102:1113	arg1	CaCl2					1123:1127	1M CaCl2	1120:1127	1M CaCl2	1120:1127	Neither the plotting process nor the crosslinking with 1M CaCl2 denatured the proteins.
27716641	3	51	theme	structures	736:745	arg1	plotting					712:719	3D plotting	709:719	3D plotting of open-porous structures without an additional mechanical support	709:786	Alginate, chitosan, gellan gum, gelatin and collagen hydrogels were utilized successfully as core materials-hydrogels which are too soft for 3D plotting of open-porous structures without an additional mechanical support.
27716641	2	52	theme	robust	547:552	arg1	scaffolds					557:565	mechanically stable and robust 3D scaffolds	523:565	mechanically stable and robust 3D scaffolds	523:565	We now demonstrate that combining high concentrated (16.7 wt%) alginate hydrogels as shell material with low concentrated, soft biopolymer hydrogels as core leads to mechanically stable and robust 3D scaffolds.
27716641	7	53	theme	growth	1200:1205	arg1	BMP-2					1225:1229	BMP-2	1225:1229	BMP-2	1225:1229	When core and shell were loaded with different growth factors (VEGF and BMP-2, respectively) a dual release was achieved.
27716641	7	53	theme	growth	1200:1205	arg1	VEGF					1216:1219	VEGF	1216:1219	VEGF	1216:1219	When core and shell were loaded with different growth factors (VEGF and BMP-2, respectively) a dual release was achieved.
27716641	7	53	theme	growth	1200:1205	arg1	factors					1207:1213	different growth factors	1190:1213	different growth factors (VEGF and BMP-2, respectively)	1190:1244	When core and shell were loaded with different growth factors (VEGF and BMP-2, respectively) a dual release was achieved.
27716641	3	54	used	utilized	636:643	arg2	hydrogels					621:629	Alginate, chitosan, gellan gum, gelatin and collagen hydrogels	568:629	Alginate, chitosan, gellan gum, gelatin and collagen hydrogels	568:629	Alginate, chitosan, gellan gum, gelatin and collagen hydrogels were utilized successfully as core materials-hydrogels which are too soft for 3D plotting of open-porous structures without an additional mechanical support.
27716641	3	54	used	utilized	636:643	arg2	materials-hydrogels					666:684	core materials-hydrogels	661:684	core materials-hydrogels which are too soft for 3D plotting of open-porous structures without an additional mechanical support	661:786	Alginate, chitosan, gellan gum, gelatin and collagen hydrogels were utilized successfully as core materials-hydrogels which are too soft for 3D plotting of open-porous structures without an additional mechanical support.
27716641	1	55	from	fashion	217:223	arg1	extrusion					155:163	Three-dimensional extrusion	137:163	Three-dimensional extrusion of two different biomaterials in a core/shell (c/s) fashion	137:223	Three-dimensional extrusion of two different biomaterials in a core/shell (c/s) fashion has gained much interest in the last couple of years as it allows for fabricating constructs with novel and interesting properties.
27716641	1	56	dep	last	257:260	arg1	couple					262:267	couple	262:267	couple	262:267	Three-dimensional extrusion of two different biomaterials in a core/shell (c/s) fashion has gained much interest in the last couple of years as it allows for fabricating constructs with novel and interesting properties.
27716641	3	57	theme	gellan	588:593	arg1	gum					595:597	gellan gum	588:597	gellan gum	588:597	Alginate, chitosan, gellan gum, gelatin and collagen hydrogels were utilized successfully as core materials-hydrogels which are too soft for 3D plotting of open-porous structures without an additional mechanical support.
27716641	4	58	theme	respective	793:802	arg1	scaffolds					808:816	The respective c/s scaffolds	789:816	The respective c/s scaffolds	789:816	The respective c/s scaffolds were characterized concerning their morphology, mechanical properties and swelling behavior.
27716641	3	59	theme	3D	709:710	arg1	plotting					712:719	3D plotting	709:719	3D plotting of open-porous structures without an additional mechanical support	709:786	Alginate, chitosan, gellan gum, gelatin and collagen hydrogels were utilized successfully as core materials-hydrogels which are too soft for 3D plotting of open-porous structures without an additional mechanical support.
27716641	2	60	theme	stable	536:541	arg1	scaffolds					557:565	mechanically stable and robust 3D scaffolds	523:565	mechanically stable and robust 3D scaffolds	523:565	We now demonstrate that combining high concentrated (16.7 wt%) alginate hydrogels as shell material with low concentrated, soft biopolymer hydrogels as core leads to mechanically stable and robust 3D scaffolds.
27716641	5	61	theme	core	1027:1030	arg1	composition					1032:1042	core composition	1027:1042	core composition	1027:1042	It could be shown that core as well as shell part can be loaded with growth factors and that the release depends on core composition and shell thickness.
27716641	3	62	theme	gum	595:597	arg1	materials-hydrogels					666:684	core materials-hydrogels	661:684	core materials-hydrogels which are too soft for 3D plotting of open-porous structures without an additional mechanical support	661:786	Alginate, chitosan, gellan gum, gelatin and collagen hydrogels were utilized successfully as core materials-hydrogels which are too soft for 3D plotting of open-porous structures without an additional mechanical support.
27716641	3	62	theme	gum	595:597	arg1	hydrogels					621:629	Alginate, chitosan, gellan gum, gelatin and collagen hydrogels	568:629	Alginate, chitosan, gellan gum, gelatin and collagen hydrogels	568:629	Alginate, chitosan, gellan gum, gelatin and collagen hydrogels were utilized successfully as core materials-hydrogels which are too soft for 3D plotting of open-porous structures without an additional mechanical support.
27716641	6	63	theme	plotting	1077:1084	arg1	process					1086:1092	the plotting process	1073:1092	the plotting process	1073:1092	Neither the plotting process nor the crosslinking with 1M CaCl2 denatured the proteins.
27716641	0	64	theme	core/shell	60:69	arg1	fashion					71:77	a core/shell fashion	58:77	a core/shell fashion by 3D plotting to achieve mechanically robust constructs	58:134	A versatile method for combining different biopolymers in a core/shell fashion by 3D plotting to achieve mechanically robust constructs.
27716641	1	65	dep	core/shell	200:209	arg1	c/s					212:214	c/s	212:214	c/s	212:214	Three-dimensional extrusion of two different biomaterials in a core/shell (c/s) fashion has gained much interest in the last couple of years as it allows for fabricating constructs with novel and interesting properties.
27716641	1	66	theme	Three-dimensional	137:153	arg1	extrusion					155:163	Three-dimensional extrusion	137:163	Three-dimensional extrusion of two different biomaterials in a core/shell (c/s) fashion	137:223	Three-dimensional extrusion of two different biomaterials in a core/shell (c/s) fashion has gained much interest in the last couple of years as it allows for fabricating constructs with novel and interesting properties.
27716641	1	67	theme	gained	229:234	arg1	interest					241:248	gained much interest	229:248	gained much interest	229:248	Three-dimensional extrusion of two different biomaterials in a core/shell (c/s) fashion has gained much interest in the last couple of years as it allows for fabricating constructs with novel and interesting properties.
27716641	2	68	theme	concentrated	466:477	arg1	hydrogels					496:504	low concentrated, soft biopolymer hydrogels	462:504	low concentrated, soft biopolymer hydrogels as core	462:512	We now demonstrate that combining high concentrated (16.7 wt%) alginate hydrogels as shell material with low concentrated, soft biopolymer hydrogels as core leads to mechanically stable and robust 3D scaffolds.
27716641	2	69	theme	alginate	420:427	arg1	%					417:417	16.7 wt%	410:417	16.7 wt%	410:417	We now demonstrate that combining high concentrated (16.7 wt%) alginate hydrogels as shell material with low concentrated, soft biopolymer hydrogels as core leads to mechanically stable and robust 3D scaffolds.
27716641	2	69	theme	alginate	420:427	arg1	hydrogels					429:437	high concentrated (16.7 wt%) alginate hydrogels	391:437	high concentrated (16.7 wt%) alginate hydrogels as shell material	391:455	We now demonstrate that combining high concentrated (16.7 wt%) alginate hydrogels as shell material with low concentrated, soft biopolymer hydrogels as core leads to mechanically stable and robust 3D scaffolds.
27716641	1	70	theme	much	236:239	arg1	interest					241:248	gained much interest	229:248	gained much interest	229:248	Three-dimensional extrusion of two different biomaterials in a core/shell (c/s) fashion has gained much interest in the last couple of years as it allows for fabricating constructs with novel and interesting properties.
27178928	0	0	theme	carbohydrate	87:98	arg1	additives					100:108	carbohydrate additives	87:108	carbohydrate additives	87:108	Preparation and characterization of cellulose composite hydrogels from tea residue and carbohydrate additives.
27178928	5	1	theme	mechanical	834:843	arg1	characteristics					845:859	mechanical characteristics	834:859	mechanical characteristics	834:859	Chitosan and guar gum could improve thermostability and mechanical characteristics of the composite hydrogels, while κ-carrageenan or soluble starch could improve equilibrium swelling ratio, sodium salicylate loading and releasing.
27178928	4	2	theme	prepared	758:765	arg1	hydrogels					767:775	the prepared hydrogels	754:775	the prepared hydrogels	754:775	Thiazolyl blue tetrazolium bromide assay and relative growth rates were adopted to evaluate cell cytotoxicity and biocompatibility of the prepared hydrogels.
27178928	4	3	theme	blue	630:633	arg1	assay					655:659	Thiazolyl blue tetrazolium bromide assay	620:659	Thiazolyl blue tetrazolium bromide assay	620:659	Thiazolyl blue tetrazolium bromide assay and relative growth rates were adopted to evaluate cell cytotoxicity and biocompatibility of the prepared hydrogels.
27178928	6	4	theme	Guar	1010:1013	arg1	gum					1015:1017	Guar gum	1010:1017	Guar gum	1010:1017	Guar gum and chitosan could increase permeation resistance and were beneficial for release control of the hydrogels.
27178928	7	5	theme	soluble	1177:1183	arg1	starch					1185:1190	soluble starch	1177:1190	soluble starch	1177:1190	Addition of chitosan, κ-carrageenan, guar gum and soluble starch were proven cell compatibility and non-cytotoxicity.
27178928	1	6	theme	soluble	272:278	arg1	starch					280:285	soluble starch	272:285	soluble starch	272:285	Composite hydrogels were prepared from tea cellulose in ionic liquid of 1-allyl-3-methylimidazolium chloride and effect of κ-carrageenan, chitosan, guar gum and soluble starch on characteristics of the prepared hydrogels were investigated.
27178928	5	7	theme	guar	791:794	arg1	gum					796:798	guar gum	791:798	guar gum	791:798	Chitosan and guar gum could improve thermostability and mechanical characteristics of the composite hydrogels, while κ-carrageenan or soluble starch could improve equilibrium swelling ratio, sodium salicylate loading and releasing.
27178928	1	8	theme	starch	280:285	arg1	effect					224:229	effect	224:229	effect of κ-carrageenan, chitosan, guar gum and soluble starch on characteristics of the prepared hydrogels	224:330	Composite hydrogels were prepared from tea cellulose in ionic liquid of 1-allyl-3-methylimidazolium chloride and effect of κ-carrageenan, chitosan, guar gum and soluble starch on characteristics of the prepared hydrogels were investigated.
27178928	7	9	theme	cell	1204:1207	arg1	compatibility					1209:1221	cell compatibility	1204:1221	cell compatibility	1204:1221	Addition of chitosan, κ-carrageenan, guar gum and soluble starch were proven cell compatibility and non-cytotoxicity.
27178928	4	10	theme	tetrazolium	635:645	arg1	assay					655:659	Thiazolyl blue tetrazolium bromide assay	620:659	Thiazolyl blue tetrazolium bromide assay	620:659	Thiazolyl blue tetrazolium bromide assay and relative growth rates were adopted to evaluate cell cytotoxicity and biocompatibility of the prepared hydrogels.
27178928	7	11	theme	starch	1185:1190	arg1	Addition					1127:1134	Addition	1127:1134	Addition of chitosan, κ-carrageenan, guar gum and soluble starch	1127:1190	Addition of chitosan, κ-carrageenan, guar gum and soluble starch were proven cell compatibility and non-cytotoxicity.
27178928	3	12	theme	model	521:525	arg1	salicylate					494:503	Sodium salicylate	487:503	Sodium salicylate	487:503	Sodium salicylate was used as the model drug to compare the swelling, drug loading and releasing kinetics of the prepared hydrogels.
27178928	3	12	theme	model	521:525	arg1	drug					527:530	the model drug	517:530	the model drug	517:530	Sodium salicylate was used as the model drug to compare the swelling, drug loading and releasing kinetics of the prepared hydrogels.
27178928	2	13	dep	transform	405:413	arg1	infrared					415:422	infrared	415:422	transform infrared	405:422	The prepared hydrogels were characterized via Fourier transform infrared, thermogravimetry analysis, differential scanning calorimetry.
27178928	5	14	theme	ratio	962:966	arg1	loading					987:993	equilibrium swelling ratio, sodium salicylate loading	941:993	equilibrium swelling ratio, sodium salicylate loading	941:993	Chitosan and guar gum could improve thermostability and mechanical characteristics of the composite hydrogels, while κ-carrageenan or soluble starch could improve equilibrium swelling ratio, sodium salicylate loading and releasing.
27178928	4	15	theme	cell	712:715	arg1	cytotoxicity					717:728	cell cytotoxicity	712:728	cell cytotoxicity	712:728	Thiazolyl blue tetrazolium bromide assay and relative growth rates were adopted to evaluate cell cytotoxicity and biocompatibility of the prepared hydrogels.
27178928	5	16	theme	hydrogels	878:886	arg1	thermostability					814:828	thermostability	814:828	thermostability	814:828	Chitosan and guar gum could improve thermostability and mechanical characteristics of the composite hydrogels, while κ-carrageenan or soluble starch could improve equilibrium swelling ratio, sodium salicylate loading and releasing.
27178928	5	16	theme	hydrogels	878:886	arg1	characteristics					845:859	mechanical characteristics	834:859	mechanical characteristics	834:859	Chitosan and guar gum could improve thermostability and mechanical characteristics of the composite hydrogels, while κ-carrageenan or soluble starch could improve equilibrium swelling ratio, sodium salicylate loading and releasing.
27178928	2	17	dep	Fourier	397:403	arg1	transform					405:413	transform	405:413	transform infrared	405:422	The prepared hydrogels were characterized via Fourier transform infrared, thermogravimetry analysis, differential scanning calorimetry.
27178928	1	18	theme	1-allyl-3-methylimidazolium	183:209	arg1	chloride					211:218	1-allyl-3-methylimidazolium chloride	183:218	1-allyl-3-methylimidazolium chloride	183:218	Composite hydrogels were prepared from tea cellulose in ionic liquid of 1-allyl-3-methylimidazolium chloride and effect of κ-carrageenan, chitosan, guar gum and soluble starch on characteristics of the prepared hydrogels were investigated.
27178928	6	19	theme	permeation	1047:1056	arg1	resistance					1058:1067	permeation resistance	1047:1067	permeation resistance	1047:1067	Guar gum and chitosan could increase permeation resistance and were beneficial for release control of the hydrogels.
27178928	3	20	theme	hydrogels	609:617	arg1	kinetics					584:591	releasing kinetics	574:591	releasing kinetics	574:591	Sodium salicylate was used as the model drug to compare the swelling, drug loading and releasing kinetics of the prepared hydrogels.
27178928	3	20	theme	hydrogels	609:617	arg1	swelling					547:554	swelling	547:554	swelling	547:554	Sodium salicylate was used as the model drug to compare the swelling, drug loading and releasing kinetics of the prepared hydrogels.
27178928	3	20	theme	hydrogels	609:617	arg1	loading					562:568	drug loading	557:568	drug loading	557:568	Sodium salicylate was used as the model drug to compare the swelling, drug loading and releasing kinetics of the prepared hydrogels.
27178928	1	21	theme	chloride	211:218	arg1	liquid					173:178	liquid	173:178	liquid	173:178	Composite hydrogels were prepared from tea cellulose in ionic liquid of 1-allyl-3-methylimidazolium chloride and effect of κ-carrageenan, chitosan, guar gum and soluble starch on characteristics of the prepared hydrogels were investigated.
27178928	6	22	theme	release	1093:1099	arg1	control					1101:1107	release control	1093:1107	release control of the hydrogels	1093:1124	Guar gum and chitosan could increase permeation resistance and were beneficial for release control of the hydrogels.
27178928	5	23	theme	equilibrium	941:951	arg1	ratio					962:966	equilibrium swelling ratio	941:966	equilibrium swelling ratio	941:966	Chitosan and guar gum could improve thermostability and mechanical characteristics of the composite hydrogels, while κ-carrageenan or soluble starch could improve equilibrium swelling ratio, sodium salicylate loading and releasing.
27178928	5	23	theme	equilibrium	941:951	arg1	salicylate					976:985	sodium salicylate	969:985	sodium salicylate	969:985	Chitosan and guar gum could improve thermostability and mechanical characteristics of the composite hydrogels, while κ-carrageenan or soluble starch could improve equilibrium swelling ratio, sodium salicylate loading and releasing.
27178928	3	24	used	used	509:512	arg2	drug					527:530	the model drug	517:530	the model drug	517:530	Sodium salicylate was used as the model drug to compare the swelling, drug loading and releasing kinetics of the prepared hydrogels.
27178928	3	24	used	used	509:512	arg2	salicylate					494:503	Sodium salicylate	487:503	Sodium salicylate	487:503	Sodium salicylate was used as the model drug to compare the swelling, drug loading and releasing kinetics of the prepared hydrogels.
27178928	3	25	theme	Sodium	487:492	arg1	salicylate					494:503	Sodium salicylate	487:503	Sodium salicylate	487:503	Sodium salicylate was used as the model drug to compare the swelling, drug loading and releasing kinetics of the prepared hydrogels.
27178928	3	25	theme	Sodium	487:492	arg1	drug					527:530	the model drug	517:530	the model drug	517:530	Sodium salicylate was used as the model drug to compare the swelling, drug loading and releasing kinetics of the prepared hydrogels.
27178928	5	26	theme	κ-carrageenan	895:907	arg1	starch					920:925	κ-carrageenan or soluble starch	895:925	κ-carrageenan or soluble starch	895:925	Chitosan and guar gum could improve thermostability and mechanical characteristics of the composite hydrogels, while κ-carrageenan or soluble starch could improve equilibrium swelling ratio, sodium salicylate loading and releasing.
27178928	7	27	theme	guar	1164:1167	arg1	gum					1169:1171	guar gum	1164:1171	guar gum	1164:1171	Addition of chitosan, κ-carrageenan, guar gum and soluble starch were proven cell compatibility and non-cytotoxicity.
27178928	1	28	theme	Composite	111:119	arg1	hydrogels					121:129	Composite hydrogels	111:129	Composite hydrogels	111:129	Composite hydrogels were prepared from tea cellulose in ionic liquid of 1-allyl-3-methylimidazolium chloride and effect of κ-carrageenan, chitosan, guar gum and soluble starch on characteristics of the prepared hydrogels were investigated.
27178928	5	29	theme	soluble	912:918	arg1	starch					920:925	κ-carrageenan or soluble starch	895:925	κ-carrageenan or soluble starch	895:925	Chitosan and guar gum could improve thermostability and mechanical characteristics of the composite hydrogels, while κ-carrageenan or soluble starch could improve equilibrium swelling ratio, sodium salicylate loading and releasing.
27178928	7	30	theme	gum	1169:1171	arg1	Addition					1127:1134	Addition	1127:1134	Addition of chitosan, κ-carrageenan, guar gum and soluble starch	1127:1190	Addition of chitosan, κ-carrageenan, guar gum and soluble starch were proven cell compatibility and non-cytotoxicity.
27178928	3	31	theme	prepared	600:607	arg1	hydrogels					609:617	the prepared hydrogels	596:617	the prepared hydrogels	596:617	Sodium salicylate was used as the model drug to compare the swelling, drug loading and releasing kinetics of the prepared hydrogels.
27178928	2	32	theme	scanning	465:472	arg1	Fourier					397:403	Fourier	397:403	Fourier transform infrared	397:422	The prepared hydrogels were characterized via Fourier transform infrared, thermogravimetry analysis, differential scanning calorimetry.
27178928	2	32	theme	scanning	465:472	arg1	calorimetry					474:484	differential scanning calorimetry	452:484	differential scanning calorimetry	452:484	The prepared hydrogels were characterized via Fourier transform infrared, thermogravimetry analysis, differential scanning calorimetry.
27178928	0	33	theme	composite	46:54	arg1	hydrogels					56:64	cellulose composite hydrogels	36:64	cellulose composite hydrogels	36:64	Preparation and characterization of cellulose composite hydrogels from tea residue and carbohydrate additives.
27178928	1	34	theme	prepared	313:320	arg1	hydrogels					322:330	the prepared hydrogels	309:330	the prepared hydrogels	309:330	Composite hydrogels were prepared from tea cellulose in ionic liquid of 1-allyl-3-methylimidazolium chloride and effect of κ-carrageenan, chitosan, guar gum and soluble starch on characteristics of the prepared hydrogels were investigated.
27178928	7	35	theme	κ-carrageenan	1149:1161	arg1	Addition					1127:1134	Addition	1127:1134	Addition of chitosan, κ-carrageenan, guar gum and soluble starch	1127:1190	Addition of chitosan, κ-carrageenan, guar gum and soluble starch were proven cell compatibility and non-cytotoxicity.
27178928	2	36	theme	differential	452:463	arg1	Fourier					397:403	Fourier	397:403	Fourier transform infrared	397:422	The prepared hydrogels were characterized via Fourier transform infrared, thermogravimetry analysis, differential scanning calorimetry.
27178928	2	36	theme	differential	452:463	arg1	calorimetry					474:484	differential scanning calorimetry	452:484	differential scanning calorimetry	452:484	The prepared hydrogels were characterized via Fourier transform infrared, thermogravimetry analysis, differential scanning calorimetry.
27178928	1	37	theme	κ-carrageenan	234:246	arg1	effect					224:229	effect	224:229	effect of κ-carrageenan, chitosan, guar gum and soluble starch on characteristics of the prepared hydrogels	224:330	Composite hydrogels were prepared from tea cellulose in ionic liquid of 1-allyl-3-methylimidazolium chloride and effect of κ-carrageenan, chitosan, guar gum and soluble starch on characteristics of the prepared hydrogels were investigated.
27178928	0	38	theme	cellulose	36:44	arg1	hydrogels					56:64	cellulose composite hydrogels	36:64	cellulose composite hydrogels	36:64	Preparation and characterization of cellulose composite hydrogels from tea residue and carbohydrate additives.
27178928	1	39	theme	hydrogels	322:330	arg1	characteristics					290:304	characteristics	290:304	characteristics of the prepared hydrogels	290:330	Composite hydrogels were prepared from tea cellulose in ionic liquid of 1-allyl-3-methylimidazolium chloride and effect of κ-carrageenan, chitosan, guar gum and soluble starch on characteristics of the prepared hydrogels were investigated.
27178928	4	40	theme	Thiazolyl	620:628	arg1	assay					655:659	Thiazolyl blue tetrazolium bromide assay	620:659	Thiazolyl blue tetrazolium bromide assay	620:659	Thiazolyl blue tetrazolium bromide assay and relative growth rates were adopted to evaluate cell cytotoxicity and biocompatibility of the prepared hydrogels.
27178928	0	41	from	residue	75:81	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of cellulose composite hydrogels from tea residue and carbohydrate additives.
27178928	0	41	from	residue	75:81	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of cellulose composite hydrogels from tea residue and carbohydrate additives.
27178928	4	42	theme	growth	674:679	arg1	rates					681:685	relative growth rates	665:685	relative growth rates	665:685	Thiazolyl blue tetrazolium bromide assay and relative growth rates were adopted to evaluate cell cytotoxicity and biocompatibility of the prepared hydrogels.
27178928	5	43	theme	composite	868:876	arg1	hydrogels					878:886	the composite hydrogels	864:886	the composite hydrogels	864:886	Chitosan and guar gum could improve thermostability and mechanical characteristics of the composite hydrogels, while κ-carrageenan or soluble starch could improve equilibrium swelling ratio, sodium salicylate loading and releasing.
27178928	5	44	theme	sodium	969:974	arg1	ratio					962:966	equilibrium swelling ratio	941:966	equilibrium swelling ratio	941:966	Chitosan and guar gum could improve thermostability and mechanical characteristics of the composite hydrogels, while κ-carrageenan or soluble starch could improve equilibrium swelling ratio, sodium salicylate loading and releasing.
27178928	5	44	theme	sodium	969:974	arg1	salicylate					976:985	sodium salicylate	969:985	sodium salicylate	969:985	Chitosan and guar gum could improve thermostability and mechanical characteristics of the composite hydrogels, while κ-carrageenan or soluble starch could improve equilibrium swelling ratio, sodium salicylate loading and releasing.
27178928	7	45	theme	chitosan	1139:1146	arg1	Addition					1127:1134	Addition	1127:1134	Addition of chitosan, κ-carrageenan, guar gum and soluble starch	1127:1190	Addition of chitosan, κ-carrageenan, guar gum and soluble starch were proven cell compatibility and non-cytotoxicity.
27178928	0	46	theme	hydrogels	56:64	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of cellulose composite hydrogels from tea residue and carbohydrate additives.
27178928	0	46	theme	hydrogels	56:64	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of cellulose composite hydrogels from tea residue and carbohydrate additives.
27178928	4	47	theme	bromide	647:653	arg1	assay					655:659	Thiazolyl blue tetrazolium bromide assay	620:659	Thiazolyl blue tetrazolium bromide assay	620:659	Thiazolyl blue tetrazolium bromide assay and relative growth rates were adopted to evaluate cell cytotoxicity and biocompatibility of the prepared hydrogels.
27178928	1	48	from	effect	224:229	arg1	characteristics					290:304	characteristics	290:304	characteristics of the prepared hydrogels	290:330	Composite hydrogels were prepared from tea cellulose in ionic liquid of 1-allyl-3-methylimidazolium chloride and effect of κ-carrageenan, chitosan, guar gum and soluble starch on characteristics of the prepared hydrogels were investigated.
27178928	4	49	theme	hydrogels	767:775	arg1	biocompatibility					734:749	biocompatibility	734:749	biocompatibility	734:749	Thiazolyl blue tetrazolium bromide assay and relative growth rates were adopted to evaluate cell cytotoxicity and biocompatibility of the prepared hydrogels.
27178928	4	49	theme	hydrogels	767:775	arg1	cytotoxicity					717:728	cell cytotoxicity	712:728	cell cytotoxicity	712:728	Thiazolyl blue tetrazolium bromide assay and relative growth rates were adopted to evaluate cell cytotoxicity and biocompatibility of the prepared hydrogels.
27178928	2	50	theme	prepared	355:362	arg1	hydrogels					364:372	The prepared hydrogels	351:372	The prepared hydrogels	351:372	The prepared hydrogels were characterized via Fourier transform infrared, thermogravimetry analysis, differential scanning calorimetry.
27178928	1	51	theme	chitosan	249:256	arg1	effect					224:229	effect	224:229	effect of κ-carrageenan, chitosan, guar gum and soluble starch on characteristics of the prepared hydrogels	224:330	Composite hydrogels were prepared from tea cellulose in ionic liquid of 1-allyl-3-methylimidazolium chloride and effect of κ-carrageenan, chitosan, guar gum and soluble starch on characteristics of the prepared hydrogels were investigated.
27178928	3	52	theme	releasing	574:582	arg1	kinetics					584:591	releasing kinetics	574:591	releasing kinetics	574:591	Sodium salicylate was used as the model drug to compare the swelling, drug loading and releasing kinetics of the prepared hydrogels.
27178928	1	53	theme	tea	150:152	arg1	cellulose					154:162	tea cellulose	150:162	tea cellulose	150:162	Composite hydrogels were prepared from tea cellulose in ionic liquid of 1-allyl-3-methylimidazolium chloride and effect of κ-carrageenan, chitosan, guar gum and soluble starch on characteristics of the prepared hydrogels were investigated.
27178928	6	54	theme	hydrogels	1116:1124	arg1	control					1101:1107	release control	1093:1107	release control of the hydrogels	1093:1124	Guar gum and chitosan could increase permeation resistance and were beneficial for release control of the hydrogels.
27178928	0	55	from	additives	100:108	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of cellulose composite hydrogels from tea residue and carbohydrate additives.
27178928	0	55	from	additives	100:108	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of cellulose composite hydrogels from tea residue and carbohydrate additives.
27178928	3	56	theme	drug	557:560	arg1	loading					562:568	drug loading	557:568	drug loading	557:568	Sodium salicylate was used as the model drug to compare the swelling, drug loading and releasing kinetics of the prepared hydrogels.
27178928	2	57	theme	thermogravimetry	425:440	arg1	analysis					442:449	thermogravimetry analysis	425:449	thermogravimetry analysis	425:449	The prepared hydrogels were characterized via Fourier transform infrared, thermogravimetry analysis, differential scanning calorimetry.
27178928	2	57	theme	thermogravimetry	425:440	arg1	Fourier					397:403	Fourier	397:403	Fourier transform infrared	397:422	The prepared hydrogels were characterized via Fourier transform infrared, thermogravimetry analysis, differential scanning calorimetry.
27178928	1	58	theme	guar	259:262	arg1	gum					264:266	guar gum	259:266	guar gum	259:266	Composite hydrogels were prepared from tea cellulose in ionic liquid of 1-allyl-3-methylimidazolium chloride and effect of κ-carrageenan, chitosan, guar gum and soluble starch on characteristics of the prepared hydrogels were investigated.
27178928	0	59	theme	tea	71:73	arg1	residue					75:81	tea residue	71:81	tea residue	71:81	Preparation and characterization of cellulose composite hydrogels from tea residue and carbohydrate additives.
27178928	4	60	theme	relative	665:672	arg1	rates					681:685	relative growth rates	665:685	relative growth rates	665:685	Thiazolyl blue tetrazolium bromide assay and relative growth rates were adopted to evaluate cell cytotoxicity and biocompatibility of the prepared hydrogels.
27178928	5	61	theme	swelling	953:960	arg1	ratio					962:966	equilibrium swelling ratio	941:966	equilibrium swelling ratio	941:966	Chitosan and guar gum could improve thermostability and mechanical characteristics of the composite hydrogels, while κ-carrageenan or soluble starch could improve equilibrium swelling ratio, sodium salicylate loading and releasing.
27178928	5	61	theme	swelling	953:960	arg1	salicylate					976:985	sodium salicylate	969:985	sodium salicylate	969:985	Chitosan and guar gum could improve thermostability and mechanical characteristics of the composite hydrogels, while κ-carrageenan or soluble starch could improve equilibrium swelling ratio, sodium salicylate loading and releasing.
27178928	1	62	theme	gum	264:266	arg1	effect					224:229	effect	224:229	effect of κ-carrageenan, chitosan, guar gum and soluble starch on characteristics of the prepared hydrogels	224:330	Composite hydrogels were prepared from tea cellulose in ionic liquid of 1-allyl-3-methylimidazolium chloride and effect of κ-carrageenan, chitosan, guar gum and soluble starch on characteristics of the prepared hydrogels were investigated.
27393427	5	0	theme	diclofenac	791:800	arg1	drug					820:823	a model drug	812:823	a model drug	812:823	It was also studied for potential drug carrier system using diclofenac sodium as a model drug.
27393427	5	0	theme	diclofenac	791:800	arg1	sodium					802:807	diclofenac sodium	791:807	diclofenac sodium	791:807	It was also studied for potential drug carrier system using diclofenac sodium as a model drug.
27393427	9	1	from	particles	1053:1061	arg1	nanocomposite					1070:1082	the nanocomposite	1066:1082	the nanocomposite	1066:1082	The average diameters of particles in the nanocomposite were found to be around 80 nm from both XRD report, calculated by applying Scherrer equation and zeta sizer.
27393427	1	2	theme	present	163:169	arg1	project					180:186	the present research project	159:186	the present research project	159:186	The objectives of the present research project were to formulate, evaluate and perform antimicrobial study and drug delivery behavior of nanocomposite material based on biopolymer chitosan and organically modified montmorillonite clay; i.e. cloisite 10A.
27393427	1	3	theme	delivery	257:264	arg1	behavior					266:273	drug delivery behavior	252:273	drug delivery behavior	252:273	The objectives of the present research project were to formulate, evaluate and perform antimicrobial study and drug delivery behavior of nanocomposite material based on biopolymer chitosan and organically modified montmorillonite clay; i.e. cloisite 10A.
27393427	12	4	theme	further	1561:1567	arg1	research					1569:1576	further research	1561:1576	further research on the polymer/clay nanocomposites for biomedical and drug delivery applications	1561:1657	This research work provides a platform for further research on the polymer/clay nanocomposites for biomedical and drug delivery applications.
27393427	10	5	dep	report	1220:1225	arg1	revealed					1227:1234	revealed	1227:1234	report revealed that nanocomposite exhibited stronger inhibition against the microorganisms as compared to that of pure chitosan	1220:1347	The antimicrobial activity report revealed that nanocomposite exhibited stronger inhibition against the microorganisms as compared to that of pure chitosan.
27393427	1	6	theme	research	171:178	arg1	project					180:186	the present research project	159:186	the present research project	159:186	The objectives of the present research project were to formulate, evaluate and perform antimicrobial study and drug delivery behavior of nanocomposite material based on biopolymer chitosan and organically modified montmorillonite clay; i.e. cloisite 10A.
27393427	11	7	dep	in	1359:1360	arg1	vitro					1362:1366	vitro	1362:1366	vitro	1362:1366	From the in vitro drug-release study, it is observed that biopolymer/clay nanocomposite exhibited extended release period of drug as compared to the pristine chitosan.
27393427	1	8	theme	i.e.	377:380	arg1	10A					391:393	i.e. cloisite 10A	377:393	i.e. cloisite 10A	377:393	The objectives of the present research project were to formulate, evaluate and perform antimicrobial study and drug delivery behavior of nanocomposite material based on biopolymer chitosan and organically modified montmorillonite clay; i.e. cloisite 10A.
27393427	6	9	theme	incorporation	831:843	arg1	efficiency					845:854	Drug incorporation efficiency	826:854	Drug incorporation efficiency	826:854	Drug incorporation efficiency and drug content were also determined.
27393427	9	10	theme	particles	1053:1061	arg1	diameters					1040:1048	The average diameters	1028:1048	The average diameters of particles in the nanocomposite	1028:1082	The average diameters of particles in the nanocomposite were found to be around 80 nm from both XRD report, calculated by applying Scherrer equation and zeta sizer.
27393427	4	11	theme	gram-	687:691	arg1	bacteria					721:728	both gram- negative and gram- positive bacteria	682:728	both gram- negative and gram- positive bacteria	682:728	Polymer/clay nanocomposite material is evaluated for its antimicrobial activity against both gram- negative and gram- positive bacteria.
27393427	4	12	theme	nanocomposite	607:619	arg1	material					621:628	Polymer/clay nanocomposite material	594:628	Polymer/clay nanocomposite material	594:628	Polymer/clay nanocomposite material is evaluated for its antimicrobial activity against both gram- negative and gram- positive bacteria.
27393427	5	13	theme	potential	755:763	arg1	system					778:783	potential drug carrier system	755:783	potential drug carrier system using diclofenac sodium as a model drug	755:823	It was also studied for potential drug carrier system using diclofenac sodium as a model drug.
27393427	1	14	theme	cloisite	382:389	arg1	10A					391:393	i.e. cloisite 10A	377:393	i.e. cloisite 10A	377:393	The objectives of the present research project were to formulate, evaluate and perform antimicrobial study and drug delivery behavior of nanocomposite material based on biopolymer chitosan and organically modified montmorillonite clay; i.e. cloisite 10A.
27393427	6	15	theme	Drug	826:829	arg1	efficiency					845:854	Drug incorporation efficiency	826:854	Drug incorporation efficiency	826:854	Drug incorporation efficiency and drug content were also determined.
27393427	3	16	theme	sizer	573:577	arg1	zeta					568:571	zeta sizer	568:577	zeta sizer	568:577	The nanocomposite material was characterized by FTIR, zeta sizer, XRD, and SEM.
27393427	1	17	theme	nanocomposite	278:290	arg1	material					292:299	nanocomposite material	278:299	nanocomposite material	278:299	The objectives of the present research project were to formulate, evaluate and perform antimicrobial study and drug delivery behavior of nanocomposite material based on biopolymer chitosan and organically modified montmorillonite clay; i.e. cloisite 10A.
27393427	0	18	theme	delivery	122:129	arg1	behavior					131:138	drug delivery behavior	117:138	drug delivery behavior	117:138	Antimicrobial activity of novel chitosan/cloisite 10A nanocomposite: Preparation, optimization, characterization and drug delivery behavior.
27393427	10	19	theme	pure	1335:1338	arg1	chitosan					1340:1347	pure chitosan	1335:1347	pure chitosan	1335:1347	The antimicrobial activity report revealed that nanocomposite exhibited stronger inhibition against the microorganisms as compared to that of pure chitosan.
27393427	1	20	theme	project	180:186	arg1	objectives					145:154	The objectives	141:154	The objectives of the present research project	141:186	The objectives of the present research project were to formulate, evaluate and perform antimicrobial study and drug delivery behavior of nanocomposite material based on biopolymer chitosan and organically modified montmorillonite clay; i.e. cloisite 10A.
27393427	0	21	theme	drug	117:120	arg1	behavior					131:138	drug delivery behavior	117:138	drug delivery behavior	117:138	Antimicrobial activity of novel chitosan/cloisite 10A nanocomposite: Preparation, optimization, characterization and drug delivery behavior.
27393427	12	22	theme	polymer/clay	1585:1596	arg1	nanocomposites					1598:1611	the polymer/clay nanocomposites	1581:1611	the polymer/clay nanocomposites for biomedical and drug delivery applications	1581:1657	This research work provides a platform for further research on the polymer/clay nanocomposites for biomedical and drug delivery applications.
27393427	9	23	theme	average	1032:1038	arg1	diameters					1040:1048	The average diameters	1028:1048	The average diameters of particles in the nanocomposite	1028:1082	The average diameters of particles in the nanocomposite were found to be around 80 nm from both XRD report, calculated by applying Scherrer equation and zeta sizer.
27393427	5	24	theme	drug	765:768	arg1	system					778:783	potential drug carrier system	755:783	potential drug carrier system using diclofenac sodium as a model drug	755:823	It was also studied for potential drug carrier system using diclofenac sodium as a model drug.
27393427	12	25	from	research	1569:1576	arg1	nanocomposites					1598:1611	the polymer/clay nanocomposites	1581:1611	the polymer/clay nanocomposites for biomedical and drug delivery applications	1581:1657	This research work provides a platform for further research on the polymer/clay nanocomposites for biomedical and drug delivery applications.
27393427	9	26	from	sizer	1186:1190	arg1	nm					1111:1112	80 nm	1108:1112	80 nm	1108:1112	The average diameters of particles in the nanocomposite were found to be around 80 nm from both XRD report, calculated by applying Scherrer equation and zeta sizer.
27393427	9	27	from	nanocomposite	1070:1082	arg1	diameters					1040:1048	The average diameters	1028:1048	The average diameters of particles in the nanocomposite	1028:1082	The average diameters of particles in the nanocomposite were found to be around 80 nm from both XRD report, calculated by applying Scherrer equation and zeta sizer.
27393427	2	28	theme	present	403:409	arg1	study					411:415	the present study	399:415	the present study	399:415	In the present study, chitosan / cloisite 10A nanocomposite material was formulated by solution mixing and optimized.
27393427	0	29	theme	Antimicrobial	0:12	arg1	activity					14:21	Antimicrobial activity	0:21	Antimicrobial activity of novel chitosan/cloisite 10A nanocomposite: Preparation, optimization, characterization and drug delivery behavior.	0:139	Antimicrobial activity of novel chitosan/cloisite 10A nanocomposite: Preparation, optimization, characterization and drug delivery behavior.
27393427	1	30	theme	material	292:299	arg1	behavior					266:273	drug delivery behavior	252:273	drug delivery behavior	252:273	The objectives of the present research project were to formulate, evaluate and perform antimicrobial study and drug delivery behavior of nanocomposite material based on biopolymer chitosan and organically modified montmorillonite clay; i.e. cloisite 10A.
27393427	1	30	theme	material	292:299	arg1	study					242:246	antimicrobial study	228:246	antimicrobial study	228:246	The objectives of the present research project were to formulate, evaluate and perform antimicrobial study and drug delivery behavior of nanocomposite material based on biopolymer chitosan and organically modified montmorillonite clay; i.e. cloisite 10A.
27393427	11	31	theme	release	1457:1463	arg1	period					1465:1470	extended release period	1448:1470	extended release period of drug	1448:1478	From the in vitro drug-release study, it is observed that biopolymer/clay nanocomposite exhibited extended release period of drug as compared to the pristine chitosan.
27393427	4	32	theme	negative	693:700	arg1	bacteria					721:728	both gram- negative and gram- positive bacteria	682:728	both gram- negative and gram- positive bacteria	682:728	Polymer/clay nanocomposite material is evaluated for its antimicrobial activity against both gram- negative and gram- positive bacteria.
27393427	0	33	theme	novel	26:30	arg1	nanocomposite					54:66	novel chitosan/cloisite 10A nanocomposite	26:66	novel chitosan/cloisite 10A nanocomposite	26:66	Antimicrobial activity of novel chitosan/cloisite 10A nanocomposite: Preparation, optimization, characterization and drug delivery behavior.
27393427	12	34	theme	biomedical	1617:1626	arg1	applications					1646:1657	biomedical and drug delivery applications	1617:1657	biomedical and drug delivery applications	1617:1657	This research work provides a platform for further research on the polymer/clay nanocomposites for biomedical and drug delivery applications.
27393427	10	35	theme	antimicrobial	1197:1209	arg1	activity					1211:1218	The antimicrobial activity	1193:1218	The antimicrobial activity	1193:1218	The antimicrobial activity report revealed that nanocomposite exhibited stronger inhibition against the microorganisms as compared to that of pure chitosan.
27393427	2	36	theme	10A	438:440	arg1	material					456:463	cloisite 10A nanocomposite material	429:463	chitosan / cloisite 10A nanocomposite material	418:463	In the present study, chitosan / cloisite 10A nanocomposite material was formulated by solution mixing and optimized.
27393427	2	37	theme	nanocomposite	442:454	arg1	material					456:463	cloisite 10A nanocomposite material	429:463	chitosan / cloisite 10A nanocomposite material	418:463	In the present study, chitosan / cloisite 10A nanocomposite material was formulated by solution mixing and optimized.
27393427	11	38	theme	drug	1475:1478	arg1	period					1465:1470	extended release period	1448:1470	extended release period of drug	1448:1478	From the in vitro drug-release study, it is observed that biopolymer/clay nanocomposite exhibited extended release period of drug as compared to the pristine chitosan.
27393427	11	39	theme	pristine	1499:1506	arg1	chitosan					1508:1515	the pristine chitosan	1495:1515	the pristine chitosan	1495:1515	From the in vitro drug-release study, it is observed that biopolymer/clay nanocomposite exhibited extended release period of drug as compared to the pristine chitosan.
27393427	2	40	theme	cloisite	429:436	arg1	material					456:463	cloisite 10A nanocomposite material	429:463	chitosan / cloisite 10A nanocomposite material	418:463	In the present study, chitosan / cloisite 10A nanocomposite material was formulated by solution mixing and optimized.
27393427	0	41	theme	10A	50:52	arg1	nanocomposite					54:66	novel chitosan/cloisite 10A nanocomposite	26:66	novel chitosan/cloisite 10A nanocomposite	26:66	Antimicrobial activity of novel chitosan/cloisite 10A nanocomposite: Preparation, optimization, characterization and drug delivery behavior.
27393427	1	42	theme	biopolymer	310:319	arg1	chitosan					321:328	biopolymer chitosan	310:328	biopolymer chitosan	310:328	The objectives of the present research project were to formulate, evaluate and perform antimicrobial study and drug delivery behavior of nanocomposite material based on biopolymer chitosan and organically modified montmorillonite clay; i.e. cloisite 10A.
27393427	0	43	theme	chitosan/cloisite	32:48	arg1	nanocomposite					54:66	novel chitosan/cloisite 10A nanocomposite	26:66	novel chitosan/cloisite 10A nanocomposite	26:66	Antimicrobial activity of novel chitosan/cloisite 10A nanocomposite: Preparation, optimization, characterization and drug delivery behavior.
27393427	11	44	theme	biopolymer/clay	1408:1422	arg1	nanocomposite					1424:1436	biopolymer/clay nanocomposite	1408:1436	biopolymer/clay nanocomposite	1408:1436	From the in vitro drug-release study, it is observed that biopolymer/clay nanocomposite exhibited extended release period of drug as compared to the pristine chitosan.
27393427	2	45	dep	chitosan	418:425	arg1	material					456:463	cloisite 10A nanocomposite material	429:463	chitosan / cloisite 10A nanocomposite material	418:463	In the present study, chitosan / cloisite 10A nanocomposite material was formulated by solution mixing and optimized.
27393427	8	46	theme	XRD	956:958	arg1	data					960:963	XRD data	956:963	XRD data	956:963	XRD data revealed the nanocrystalline composition and crystallite size.
27393427	5	47	theme	carrier	770:776	arg1	system					778:783	potential drug carrier system	755:783	potential drug carrier system using diclofenac sodium as a model drug	755:823	It was also studied for potential drug carrier system using diclofenac sodium as a model drug.
27393427	2	48	theme	solution	483:490	arg1	mixing					492:497	solution mixing	483:497	solution mixing	483:497	In the present study, chitosan / cloisite 10A nanocomposite material was formulated by solution mixing and optimized.
27393427	12	49	theme	delivery	1637:1644	arg1	applications					1646:1657	biomedical and drug delivery applications	1617:1657	biomedical and drug delivery applications	1617:1657	This research work provides a platform for further research on the polymer/clay nanocomposites for biomedical and drug delivery applications.
27393427	11	50	theme	extended	1448:1455	arg1	period					1465:1470	extended release period	1448:1470	extended release period of drug	1448:1478	From the in vitro drug-release study, it is observed that biopolymer/clay nanocomposite exhibited extended release period of drug as compared to the pristine chitosan.
27393427	9	51	from	XRD	1124:1126	arg1	nm					1111:1112	80 nm	1108:1112	80 nm	1108:1112	The average diameters of particles in the nanocomposite were found to be around 80 nm from both XRD report, calculated by applying Scherrer equation and zeta sizer.
27393427	11	52	theme	in	1359:1360	arg1	study					1381:1385	the in vitro drug-release study	1355:1385	the in vitro drug-release study	1355:1385	From the in vitro drug-release study, it is observed that biopolymer/clay nanocomposite exhibited extended release period of drug as compared to the pristine chitosan.
27393427	0	53	theme	nanocomposite	54:66	arg1	activity					14:21	Antimicrobial activity	0:21	Antimicrobial activity of novel chitosan/cloisite 10A nanocomposite: Preparation, optimization, characterization and drug delivery behavior.	0:139	Antimicrobial activity of novel chitosan/cloisite 10A nanocomposite: Preparation, optimization, characterization and drug delivery behavior.
27393427	1	54	dep	were	188:191	arg1	10A					391:393	i.e. cloisite 10A	377:393	i.e. cloisite 10A	377:393	The objectives of the present research project were to formulate, evaluate and perform antimicrobial study and drug delivery behavior of nanocomposite material based on biopolymer chitosan and organically modified montmorillonite clay; i.e. cloisite 10A.
27393427	9	55	theme	Scherrer	1159:1166	arg1	equation					1168:1175	Scherrer equation	1159:1175	Scherrer equation	1159:1175	The average diameters of particles in the nanocomposite were found to be around 80 nm from both XRD report, calculated by applying Scherrer equation and zeta sizer.
27393427	3	56	theme	nanocomposite	518:530	arg1	material					532:539	The nanocomposite material	514:539	The nanocomposite material	514:539	The nanocomposite material was characterized by FTIR, zeta sizer, XRD, and SEM.
27393427	7	57	theme	surface	936:942	arg1	topography					944:953	its surface topography	932:953	its surface topography	932:953	SEM provided the composite shape and its surface topography.
27393427	1	58	theme	modified	346:353	arg1	clay					371:374	organically modified montmorillonite clay	334:374	organically modified montmorillonite clay	334:374	The objectives of the present research project were to formulate, evaluate and perform antimicrobial study and drug delivery behavior of nanocomposite material based on biopolymer chitosan and organically modified montmorillonite clay; i.e. cloisite 10A.
27393427	12	59	theme	research	1523:1530	arg1	work					1532:1535	This research work	1518:1535	This research work	1518:1535	This research work provides a platform for further research on the polymer/clay nanocomposites for biomedical and drug delivery applications.
27393427	8	60	theme	crystallite	1010:1020	arg1	size					1022:1025	crystallite size	1010:1025	crystallite size	1010:1025	XRD data revealed the nanocrystalline composition and crystallite size.
27393427	1	61	theme	antimicrobial	228:240	arg1	study					242:246	antimicrobial study	228:246	antimicrobial study	228:246	The objectives of the present research project were to formulate, evaluate and perform antimicrobial study and drug delivery behavior of nanocomposite material based on biopolymer chitosan and organically modified montmorillonite clay; i.e. cloisite 10A.
27393427	6	62	theme	drug	860:863	arg1	content					865:871	drug content	860:871	drug content	860:871	Drug incorporation efficiency and drug content were also determined.
27393427	4	63	dep	negative	693:700	arg1	positive					712:719	positive	712:719	positive	712:719	Polymer/clay nanocomposite material is evaluated for its antimicrobial activity against both gram- negative and gram- positive bacteria.
27393427	8	64	theme	nanocrystalline	978:992	arg1	composition					994:1004	nanocrystalline composition	978:1004	nanocrystalline composition	978:1004	XRD data revealed the nanocrystalline composition and crystallite size.
27393427	12	65	theme	drug	1632:1635	arg1	applications					1646:1657	biomedical and drug delivery applications	1617:1657	biomedical and drug delivery applications	1617:1657	This research work provides a platform for further research on the polymer/clay nanocomposites for biomedical and drug delivery applications.
27393427	9	66	from	diameters	1040:1048	arg1	nanocomposite					1070:1082	the nanocomposite	1066:1082	the nanocomposite	1066:1082	The average diameters of particles in the nanocomposite were found to be around 80 nm from both XRD report, calculated by applying Scherrer equation and zeta sizer.
27393427	0	67	dep	activity	14:21	arg1	optimization					82:93	optimization	82:93	optimization	82:93	Antimicrobial activity of novel chitosan/cloisite 10A nanocomposite: Preparation, optimization, characterization and drug delivery behavior.
27393427	0	67	dep	activity	14:21	arg1	behavior					131:138	drug delivery behavior	117:138	drug delivery behavior	117:138	Antimicrobial activity of novel chitosan/cloisite 10A nanocomposite: Preparation, optimization, characterization and drug delivery behavior.
27393427	0	67	dep	activity	14:21	arg1	Preparation					69:79	Preparation	69:79	Preparation	69:79	Antimicrobial activity of novel chitosan/cloisite 10A nanocomposite: Preparation, optimization, characterization and drug delivery behavior.
27393427	0	67	dep	activity	14:21	arg1	characterization					96:111	characterization	96:111	characterization	96:111	Antimicrobial activity of novel chitosan/cloisite 10A nanocomposite: Preparation, optimization, characterization and drug delivery behavior.
27393427	4	68	theme	antimicrobial	651:663	arg1	activity					665:672	its antimicrobial activity	647:672	its antimicrobial activity against both gram- negative and gram- positive bacteria	647:728	Polymer/clay nanocomposite material is evaluated for its antimicrobial activity against both gram- negative and gram- positive bacteria.
27393427	10	69	theme	stronger	1265:1272	arg1	inhibition					1274:1283	stronger inhibition	1265:1283	stronger inhibition	1265:1283	The antimicrobial activity report revealed that nanocomposite exhibited stronger inhibition against the microorganisms as compared to that of pure chitosan.
27393427	4	70	theme	Polymer/clay	594:605	arg1	material					621:628	Polymer/clay nanocomposite material	594:628	Polymer/clay nanocomposite material	594:628	Polymer/clay nanocomposite material is evaluated for its antimicrobial activity against both gram- negative and gram- positive bacteria.
27393427	5	71	theme	model	814:818	arg1	drug					820:823	a model drug	812:823	a model drug	812:823	It was also studied for potential drug carrier system using diclofenac sodium as a model drug.
27393427	5	71	theme	model	814:818	arg1	sodium					802:807	diclofenac sodium	791:807	diclofenac sodium	791:807	It was also studied for potential drug carrier system using diclofenac sodium as a model drug.
27393427	1	72	theme	montmorillonite	355:369	arg1	clay					371:374	organically modified montmorillonite clay	334:374	organically modified montmorillonite clay	334:374	The objectives of the present research project were to formulate, evaluate and perform antimicrobial study and drug delivery behavior of nanocomposite material based on biopolymer chitosan and organically modified montmorillonite clay; i.e. cloisite 10A.
27393427	11	73	theme	drug-release	1368:1379	arg1	study					1381:1385	the in vitro drug-release study	1355:1385	the in vitro drug-release study	1355:1385	From the in vitro drug-release study, it is observed that biopolymer/clay nanocomposite exhibited extended release period of drug as compared to the pristine chitosan.
27393427	9	74	dep	XRD	1124:1126	arg1	report					1128:1133	report	1128:1133	report	1128:1133	The average diameters of particles in the nanocomposite were found to be around 80 nm from both XRD report, calculated by applying Scherrer equation and zeta sizer.
27393427	4	75	theme	gram-	706:710	arg1	bacteria					721:728	both gram- negative and gram- positive bacteria	682:728	both gram- negative and gram- positive bacteria	682:728	Polymer/clay nanocomposite material is evaluated for its antimicrobial activity against both gram- negative and gram- positive bacteria.
27393427	7	76	theme	composite	912:920	arg1	shape					922:926	the composite shape	908:926	the composite shape	908:926	SEM provided the composite shape and its surface topography.
27393427	1	77	theme	drug	252:255	arg1	behavior					266:273	drug delivery behavior	252:273	drug delivery behavior	252:273	The objectives of the present research project were to formulate, evaluate and perform antimicrobial study and drug delivery behavior of nanocomposite material based on biopolymer chitosan and organically modified montmorillonite clay; i.e. cloisite 10A.
26280926	5	0	theme	methodologies	713:725	arg1	state					696:700	state	696:700	state of the art methodologies	696:725	In this study, samples from several batches of generic enoxaparins were purchased on the US market and analyzed with state of the art methodologies, including disaccharide building blocks quantification, nuclear magnetic resonance (NMR), and a combination of orthogonal separation techniques.
26280926	6	1	theme	high-performance	879:894	arg1	chromatography					903:916	high-performance liquid chromatography	879:916	Direct high-performance liquid chromatography analysis of the different enoxaparin batches	872:961	Direct high-performance liquid chromatography analysis of the different enoxaparin batches revealed distinct process fingerprints associated with each manufacturer.
26280926	5	2	theme	several	607:613	arg1	batches					615:621	several batches	607:621	several batches of generic enoxaparins	607:644	In this study, samples from several batches of generic enoxaparins were purchased on the US market and analyzed with state of the art methodologies, including disaccharide building blocks quantification, nuclear magnetic resonance (NMR), and a combination of orthogonal separation techniques.
26280926	1	3	theme	complex	154:160	arg1	drugs					176:180	complex anticoagulant drugs	154:180	complex anticoagulant drugs	154:180	Low-molecular-weight heparins (LMWHs) are complex anticoagulant drugs, made from heparin porcine mucosa starting material.
26280926	1	3	theme	complex	154:160	arg1	heparins					133:140	Low-molecular-weight heparins	112:140	Low-molecular-weight heparins (LMWHs)	112:148	Low-molecular-weight heparins (LMWHs) are complex anticoagulant drugs, made from heparin porcine mucosa starting material.
26280926	7	4	theme	block	1059:1063	arg1	analysis					1065:1072	Disaccharide building block analysis	1037:1072	Disaccharide building block analysis	1037:1072	Disaccharide building block analysis showed differences in the degree of sulfation, the presence of glycoserine derivatives, as well as in proportions of disaccharides.
26280926	8	5	with	analysis	1273:1280	arg1	methodology					1333:1343	a partial least squares discriminant analysis methodology	1287:1343	a partial least squares discriminant analysis methodology	1287:1343	Results were compared by statistical approaches using multivariate analysis with a partial least squares discriminant analysis methodology.
26280926	6	6	theme	Direct	872:877	arg1	analysis					918:925	Direct high-performance liquid chromatography analysis	872:925	Direct high-performance liquid chromatography analysis of the different enoxaparin batches	872:961	Direct high-performance liquid chromatography analysis of the different enoxaparin batches revealed distinct process fingerprints associated with each manufacturer.
26280926	7	7	theme	glycoserine	1137:1147	arg1	derivatives					1149:1159	glycoserine derivatives	1137:1159	glycoserine derivatives	1137:1159	Disaccharide building block analysis showed differences in the degree of sulfation, the presence of glycoserine derivatives, as well as in proportions of disaccharides.
26280926	1	8	theme	anticoagulant	162:174	arg1	drugs					176:180	complex anticoagulant drugs	154:180	complex anticoagulant drugs	154:180	Low-molecular-weight heparins (LMWHs) are complex anticoagulant drugs, made from heparin porcine mucosa starting material.
26280926	1	8	theme	anticoagulant	162:174	arg1	heparins					133:140	Low-molecular-weight heparins	112:140	Low-molecular-weight heparins (LMWHs)	112:148	Low-molecular-weight heparins (LMWHs) are complex anticoagulant drugs, made from heparin porcine mucosa starting material.
26280926	8	9	theme	multivariate	1260:1271	arg1	analysis					1273:1280	multivariate analysis	1260:1280	multivariate analysis with a partial least squares discriminant analysis methodology	1260:1343	Results were compared by statistical approaches using multivariate analysis with a partial least squares discriminant analysis methodology.
26280926	7	10	from	differences	1081:1091	arg1	degree					1100:1105	the degree	1096:1105	the degree of sulfation, the presence of glycoserine derivatives	1096:1159	Disaccharide building block analysis showed differences in the degree of sulfation, the presence of glycoserine derivatives, as well as in proportions of disaccharides.
26280926	7	10	from	differences	1081:1091	arg1	proportions					1176:1186	proportions	1176:1186	proportions of disaccharides	1176:1203	Disaccharide building block analysis showed differences in the degree of sulfation, the presence of glycoserine derivatives, as well as in proportions of disaccharides.
26280926	0	11	theme	originator	92:101	arg1	product					103:109	the originator product	88:109	the originator product	88:109	Analytical and statistical comparability of generic enoxaparins from the US market with the originator product.
26280926	0	12	from	Analytical	0:9	arg1	market					76:81	the US market	69:81	the US market	69:81	Analytical and statistical comparability of generic enoxaparins from the US market with the originator product.
26280926	10	13	dep	techniques	1561:1570	arg1	both					1649:1652	both	1649:1652	both	1649:1652	These results were further confirmed by orthogonal analytical techniques, including NMR, which revealed compositional differences of oligosaccharides both in low- and high-affinity antithrombin fractions of enoxaparin.
26280926	6	14	theme	process	981:987	arg1	fingerprints					989:1000	distinct process fingerprints	972:1000	distinct process fingerprints associated with each manufacturer	972:1034	Direct high-performance liquid chromatography analysis of the different enoxaparin batches revealed distinct process fingerprints associated with each manufacturer.
26280926	5	15	theme	generic	626:632	arg1	enoxaparins					634:644	generic enoxaparins	626:644	generic enoxaparins	626:644	In this study, samples from several batches of generic enoxaparins were purchased on the US market and analyzed with state of the art methodologies, including disaccharide building blocks quantification, nuclear magnetic resonance (NMR), and a combination of orthogonal separation techniques.
26280926	10	16	theme	enoxaparin	1706:1715	arg1	fractions					1693:1701	low- and high-affinity antithrombin fractions	1657:1701	low- and high-affinity antithrombin fractions of enoxaparin	1657:1715	These results were further confirmed by orthogonal analytical techniques, including NMR, which revealed compositional differences of oligosaccharides both in low- and high-affinity antithrombin fractions of enoxaparin.
26280926	5	17	theme	separation	849:858	arg1	techniques					860:869	orthogonal separation techniques	838:869	orthogonal separation techniques	838:869	In this study, samples from several batches of generic enoxaparins were purchased on the US market and analyzed with state of the art methodologies, including disaccharide building blocks quantification, nuclear magnetic resonance (NMR), and a combination of orthogonal separation techniques.
26280926	6	18	theme	distinct	972:979	arg1	fingerprints					989:1000	distinct process fingerprints	972:1000	distinct process fingerprints associated with each manufacturer	972:1034	Direct high-performance liquid chromatography analysis of the different enoxaparin batches revealed distinct process fingerprints associated with each manufacturer.
26280926	7	19	theme	disaccharides	1191:1203	arg1	proportions					1176:1186	proportions	1176:1186	proportions of disaccharides	1176:1203	Disaccharide building block analysis showed differences in the degree of sulfation, the presence of glycoserine derivatives, as well as in proportions of disaccharides.
26280926	8	20	theme	analysis	1324:1331	arg1	methodology					1333:1343	a partial least squares discriminant analysis methodology	1287:1343	a partial least squares discriminant analysis methodology	1287:1343	Results were compared by statistical approaches using multivariate analysis with a partial least squares discriminant analysis methodology.
26280926	3	21	dep	US	369:370	arg1	Food					372:375	Food	372:375	Food	372:375	In 2010, US Food and Drug Administration approval for supplying generic enoxaparin was granted to Sandoz and subsequently to Amphastar.
26280926	3	21	dep	US	369:370	arg1	Administration					386:399	Drug Administration	381:399	Drug Administration	381:399	In 2010, US Food and Drug Administration approval for supplying generic enoxaparin was granted to Sandoz and subsequently to Amphastar.
26280926	7	22	theme	sulfation	1110:1118	arg1	degree					1100:1105	the degree	1096:1105	the degree of sulfation, the presence of glycoserine derivatives	1096:1159	Disaccharide building block analysis showed differences in the degree of sulfation, the presence of glycoserine derivatives, as well as in proportions of disaccharides.
26280926	8	23	theme	statistical	1231:1241	arg1	approaches					1243:1252	statistical approaches	1231:1252	statistical approaches using multivariate analysis with a partial least squares discriminant analysis methodology	1231:1343	Results were compared by statistical approaches using multivariate analysis with a partial least squares discriminant analysis methodology.
26280926	3	24	dep	Food	372:375	arg1	approval					401:408	approval	401:408	approval for supplying generic enoxaparin	401:441	In 2010, US Food and Drug Administration approval for supplying generic enoxaparin was granted to Sandoz and subsequently to Amphastar.
26280926	10	25	theme	orthogonal	1539:1548	arg1	techniques					1561:1570	orthogonal analytical techniques	1539:1570	orthogonal analytical techniques	1539:1570	These results were further confirmed by orthogonal analytical techniques, including NMR, which revealed compositional differences of oligosaccharides both in low- and high-affinity antithrombin fractions of enoxaparin.
26280926	10	25	theme	orthogonal	1539:1548	arg1	NMR					1583:1585	NMR	1583:1585	NMR	1583:1585	These results were further confirmed by orthogonal analytical techniques, including NMR, which revealed compositional differences of oligosaccharides both in low- and high-affinity antithrombin fractions of enoxaparin.
26280926	4	26	from	differences	529:539	arg1	composition					544:554	composition	544:554	composition of these preparations	544:576	Little is known, however, of the differences in composition of these preparations.
26280926	10	27	theme	antithrombin	1680:1691	arg1	fractions					1693:1701	low- and high-affinity antithrombin fractions	1657:1701	low- and high-affinity antithrombin fractions of enoxaparin	1657:1715	These results were further confirmed by orthogonal analytical techniques, including NMR, which revealed compositional differences of oligosaccharides both in low- and high-affinity antithrombin fractions of enoxaparin.
26280926	6	28	theme	chromatography	903:916	arg1	analysis					918:925	Direct high-performance liquid chromatography analysis	872:925	Direct high-performance liquid chromatography analysis of the different enoxaparin batches	872:961	Direct high-performance liquid chromatography analysis of the different enoxaparin batches revealed distinct process fingerprints associated with each manufacturer.
26280926	2	29	used	used	332:335	arg2	Enoxaparin					235:244	Enoxaparin sodium	235:251	Enoxaparin sodium manufactured by Sanofi	235:274	Enoxaparin sodium manufactured by Sanofi is one of the most widely prescribed LMWHs and has been used since 1993 in the USA.
26280926	2	30	theme	LMWHs	313:317	arg1	one					279:281	one	279:281	one	279:281	Enoxaparin sodium manufactured by Sanofi is one of the most widely prescribed LMWHs and has been used since 1993 in the USA.
26280926	2	30	theme	LMWHs	313:317	arg1	LMWHs					313:317	the most widely prescribed LMWHs	286:317	the most widely prescribed LMWHs	286:317	Enoxaparin sodium manufactured by Sanofi is one of the most widely prescribed LMWHs and has been used since 1993 in the USA.
26280926	5	31	theme	nuclear	783:789	arg1	NMR					811:813	NMR	811:813	NMR	811:813	In this study, samples from several batches of generic enoxaparins were purchased on the US market and analyzed with state of the art methodologies, including disaccharide building blocks quantification, nuclear magnetic resonance (NMR), and a combination of orthogonal separation techniques.
26280926	5	31	theme	nuclear	783:789	arg1	resonance					800:808	nuclear magnetic resonance	783:808	nuclear magnetic resonance (NMR)	783:814	In this study, samples from several batches of generic enoxaparins were purchased on the US market and analyzed with state of the art methodologies, including disaccharide building blocks quantification, nuclear magnetic resonance (NMR), and a combination of orthogonal separation techniques.
26280926	6	32	theme	batches	955:961	arg1	analysis					918:925	Direct high-performance liquid chromatography analysis	872:925	Direct high-performance liquid chromatography analysis of the different enoxaparin batches	872:961	Direct high-performance liquid chromatography analysis of the different enoxaparin batches revealed distinct process fingerprints associated with each manufacturer.
26280926	2	33	theme	prescribed	302:311	arg1	LMWHs					313:317	the most widely prescribed LMWHs	286:317	the most widely prescribed LMWHs	286:317	Enoxaparin sodium manufactured by Sanofi is one of the most widely prescribed LMWHs and has been used since 1993 in the USA.
26280926	1	34	theme	heparin	193:199	arg1	mucosa					209:214	heparin porcine mucosa	193:214	heparin porcine mucosa starting material	193:232	Low-molecular-weight heparins (LMWHs) are complex anticoagulant drugs, made from heparin porcine mucosa starting material.
26280926	0	35	theme	statistical	15:25	arg1	comparability					27:39	statistical comparability	15:39	statistical comparability	15:39	Analytical and statistical comparability of generic enoxaparins from the US market with the originator product.
26280926	6	36	theme	enoxaparin	944:953	arg1	batches					955:961	the different enoxaparin batches	930:961	the different enoxaparin batches	930:961	Direct high-performance liquid chromatography analysis of the different enoxaparin batches revealed distinct process fingerprints associated with each manufacturer.
26280926	5	37	theme	US	668:669	arg1	market					671:676	the US market	664:676	the US market	664:676	In this study, samples from several batches of generic enoxaparins were purchased on the US market and analyzed with state of the art methodologies, including disaccharide building blocks quantification, nuclear magnetic resonance (NMR), and a combination of orthogonal separation techniques.
26280926	5	38	theme	techniques	860:869	arg1	NMR					811:813	NMR	811:813	NMR	811:813	In this study, samples from several batches of generic enoxaparins were purchased on the US market and analyzed with state of the art methodologies, including disaccharide building blocks quantification, nuclear magnetic resonance (NMR), and a combination of orthogonal separation techniques.
26280926	5	38	theme	techniques	860:869	arg1	resonance					800:808	nuclear magnetic resonance	783:808	nuclear magnetic resonance (NMR)	783:814	In this study, samples from several batches of generic enoxaparins were purchased on the US market and analyzed with state of the art methodologies, including disaccharide building blocks quantification, nuclear magnetic resonance (NMR), and a combination of orthogonal separation techniques.
26280926	5	38	theme	techniques	860:869	arg1	quantification					767:780	quantification	767:780	quantification	767:780	In this study, samples from several batches of generic enoxaparins were purchased on the US market and analyzed with state of the art methodologies, including disaccharide building blocks quantification, nuclear magnetic resonance (NMR), and a combination of orthogonal separation techniques.
26280926	5	38	theme	techniques	860:869	arg1	combination					823:833	a combination	821:833	a combination of orthogonal separation techniques	821:869	In this study, samples from several batches of generic enoxaparins were purchased on the US market and analyzed with state of the art methodologies, including disaccharide building blocks quantification, nuclear magnetic resonance (NMR), and a combination of orthogonal separation techniques.
26280926	1	39	theme	porcine	201:207	arg1	mucosa					209:214	heparin porcine mucosa	193:214	heparin porcine mucosa starting material	193:232	Low-molecular-weight heparins (LMWHs) are complex anticoagulant drugs, made from heparin porcine mucosa starting material.
26280926	0	40	theme	generic	44:50	arg1	enoxaparins					52:62	generic enoxaparins	44:62	generic enoxaparins from the US market	44:81	Analytical and statistical comparability of generic enoxaparins from the US market with the originator product.
26280926	6	41	theme	different	934:942	arg1	batches					955:961	the different enoxaparin batches	930:961	the different enoxaparin batches	930:961	Direct high-performance liquid chromatography analysis of the different enoxaparin batches revealed distinct process fingerprints associated with each manufacturer.
26280926	10	42	theme	analytical	1550:1559	arg1	techniques					1561:1570	orthogonal analytical techniques	1539:1570	orthogonal analytical techniques	1539:1570	These results were further confirmed by orthogonal analytical techniques, including NMR, which revealed compositional differences of oligosaccharides both in low- and high-affinity antithrombin fractions of enoxaparin.
26280926	10	42	theme	analytical	1550:1559	arg1	NMR					1583:1585	NMR	1583:1585	NMR	1583:1585	These results were further confirmed by orthogonal analytical techniques, including NMR, which revealed compositional differences of oligosaccharides both in low- and high-affinity antithrombin fractions of enoxaparin.
26280926	0	43	from	market	76:81	arg1	Analytical					0:9	Analytical	0:9	Analytical	0:9	Analytical and statistical comparability of generic enoxaparins from the US market with the originator product.
26280926	0	43	from	market	76:81	arg1	comparability					27:39	statistical comparability	15:39	statistical comparability	15:39	Analytical and statistical comparability of generic enoxaparins from the US market with the originator product.
26280926	0	43	from	market	76:81	arg1	enoxaparins					52:62	generic enoxaparins	44:62	generic enoxaparins from the US market	44:81	Analytical and statistical comparability of generic enoxaparins from the US market with the originator product.
26280926	3	44	theme	generic	424:430	arg1	enoxaparin					432:441	generic enoxaparin	424:441	generic enoxaparin	424:441	In 2010, US Food and Drug Administration approval for supplying generic enoxaparin was granted to Sandoz and subsequently to Amphastar.
26280926	3	45	theme	Drug	381:384	arg1	Administration					386:399	Drug Administration	381:399	Drug Administration	381:399	In 2010, US Food and Drug Administration approval for supplying generic enoxaparin was granted to Sandoz and subsequently to Amphastar.
26280926	9	46	theme	clear	1406:1410	arg1	distinction					1412:1422	a clear distinction to be made between the enoxaparin batches according to their manufacturer	1404:1496	a clear distinction to be made between the enoxaparin batches according to their manufacturer	1404:1496	The variations were statistically significant and allowed a clear distinction to be made between the enoxaparin batches according to their manufacturer.
26280926	5	47	theme	disaccharide	738:749	arg1	building					751:758	disaccharide building	738:758	disaccharide building	738:758	In this study, samples from several batches of generic enoxaparins were purchased on the US market and analyzed with state of the art methodologies, including disaccharide building blocks quantification, nuclear magnetic resonance (NMR), and a combination of orthogonal separation techniques.
26280926	8	48	theme	squares	1303:1309	arg1	methodology					1333:1343	a partial least squares discriminant analysis methodology	1287:1343	a partial least squares discriminant analysis methodology	1287:1343	Results were compared by statistical approaches using multivariate analysis with a partial least squares discriminant analysis methodology.
26280926	10	49	theme	high-affinity	1666:1678	arg1	fractions					1693:1701	low- and high-affinity antithrombin fractions	1657:1701	low- and high-affinity antithrombin fractions of enoxaparin	1657:1715	These results were further confirmed by orthogonal analytical techniques, including NMR, which revealed compositional differences of oligosaccharides both in low- and high-affinity antithrombin fractions of enoxaparin.
26280926	0	50	with	comparability	27:39	arg1	product					103:109	the originator product	88:109	the originator product	88:109	Analytical and statistical comparability of generic enoxaparins from the US market with the originator product.
26280926	2	51	dep	Enoxaparin	235:244	arg1	sodium					246:251	sodium	246:251	Enoxaparin sodium manufactured by Sanofi	235:274	Enoxaparin sodium manufactured by Sanofi is one of the most widely prescribed LMWHs and has been used since 1993 in the USA.
26280926	7	52	theme	derivatives	1149:1159	arg1	sulfation					1110:1118	sulfation	1110:1118	sulfation	1110:1118	Disaccharide building block analysis showed differences in the degree of sulfation, the presence of glycoserine derivatives, as well as in proportions of disaccharides.
26280926	7	52	theme	derivatives	1149:1159	arg1	presence					1125:1132	the presence	1121:1132	the presence of glycoserine derivatives	1121:1159	Disaccharide building block analysis showed differences in the degree of sulfation, the presence of glycoserine derivatives, as well as in proportions of disaccharides.
26280926	10	53	theme	low-	1657:1660	arg1	fractions					1693:1701	low- and high-affinity antithrombin fractions	1657:1701	low- and high-affinity antithrombin fractions of enoxaparin	1657:1715	These results were further confirmed by orthogonal analytical techniques, including NMR, which revealed compositional differences of oligosaccharides both in low- and high-affinity antithrombin fractions of enoxaparin.
26280926	5	54	theme	enoxaparins	634:644	arg1	batches					615:621	several batches	607:621	several batches of generic enoxaparins	607:644	In this study, samples from several batches of generic enoxaparins were purchased on the US market and analyzed with state of the art methodologies, including disaccharide building blocks quantification, nuclear magnetic resonance (NMR), and a combination of orthogonal separation techniques.
26280926	1	55	theme	Low-molecular-weight	112:131	arg1	drugs					176:180	complex anticoagulant drugs	154:180	complex anticoagulant drugs	154:180	Low-molecular-weight heparins (LMWHs) are complex anticoagulant drugs, made from heparin porcine mucosa starting material.
26280926	1	55	theme	Low-molecular-weight	112:131	arg1	LMWHs					143:147	LMWHs	143:147	LMWHs	143:147	Low-molecular-weight heparins (LMWHs) are complex anticoagulant drugs, made from heparin porcine mucosa starting material.
26280926	1	55	theme	Low-molecular-weight	112:131	arg1	heparins					133:140	Low-molecular-weight heparins	112:140	Low-molecular-weight heparins (LMWHs)	112:148	Low-molecular-weight heparins (LMWHs) are complex anticoagulant drugs, made from heparin porcine mucosa starting material.
26280926	0	56	theme	enoxaparins	52:62	arg1	Analytical					0:9	Analytical	0:9	Analytical	0:9	Analytical and statistical comparability of generic enoxaparins from the US market with the originator product.
26280926	0	56	theme	enoxaparins	52:62	arg1	comparability					27:39	statistical comparability	15:39	statistical comparability	15:39	Analytical and statistical comparability of generic enoxaparins from the US market with the originator product.
26280926	6	57	theme	liquid	896:901	arg1	chromatography					903:916	high-performance liquid chromatography	879:916	Direct high-performance liquid chromatography analysis of the different enoxaparin batches	872:961	Direct high-performance liquid chromatography analysis of the different enoxaparin batches revealed distinct process fingerprints associated with each manufacturer.
26280926	10	58	theme	compositional	1603:1615	arg1	differences					1617:1627	compositional differences	1603:1627	compositional differences of oligosaccharides	1603:1647	These results were further confirmed by orthogonal analytical techniques, including NMR, which revealed compositional differences of oligosaccharides both in low- and high-affinity antithrombin fractions of enoxaparin.
26280926	10	59	theme	oligosaccharides	1632:1647	arg1	differences					1617:1627	compositional differences	1603:1627	compositional differences of oligosaccharides	1603:1647	These results were further confirmed by orthogonal analytical techniques, including NMR, which revealed compositional differences of oligosaccharides both in low- and high-affinity antithrombin fractions of enoxaparin.
26280926	8	60	theme	discriminant	1311:1322	arg1	methodology					1333:1343	a partial least squares discriminant analysis methodology	1287:1343	a partial least squares discriminant analysis methodology	1287:1343	Results were compared by statistical approaches using multivariate analysis with a partial least squares discriminant analysis methodology.
26280926	5	61	from	batches	615:621	arg1	samples					594:600	samples	594:600	samples from several batches of generic enoxaparins	594:644	In this study, samples from several batches of generic enoxaparins were purchased on the US market and analyzed with state of the art methodologies, including disaccharide building blocks quantification, nuclear magnetic resonance (NMR), and a combination of orthogonal separation techniques.
26280926	7	62	theme	Disaccharide	1037:1048	arg1	block					1059:1063	Disaccharide building block	1037:1063	Disaccharide building block analysis	1037:1072	Disaccharide building block analysis showed differences in the degree of sulfation, the presence of glycoserine derivatives, as well as in proportions of disaccharides.
26280926	0	63	with	Analytical	0:9	arg1	product					103:109	the originator product	88:109	the originator product	88:109	Analytical and statistical comparability of generic enoxaparins from the US market with the originator product.
26280926	7	64	theme	building	1050:1057	arg1	block					1059:1063	Disaccharide building block	1037:1063	Disaccharide building block analysis	1037:1072	Disaccharide building block analysis showed differences in the degree of sulfation, the presence of glycoserine derivatives, as well as in proportions of disaccharides.
26280926	4	65	theme	preparations	565:576	arg1	composition					544:554	composition	544:554	composition of these preparations	544:576	Little is known, however, of the differences in composition of these preparations.
26280926	0	66	theme	US	73:74	arg1	market					76:81	the US market	69:81	the US market	69:81	Analytical and statistical comparability of generic enoxaparins from the US market with the originator product.
26280926	8	67	theme	least	1297:1301	arg1	squares					1303:1309	partial least squares	1289:1309	a partial least squares discriminant analysis methodology	1287:1343	Results were compared by statistical approaches using multivariate analysis with a partial least squares discriminant analysis methodology.
26280926	8	68	theme	partial	1289:1295	arg1	squares					1303:1309	partial least squares	1289:1309	a partial least squares discriminant analysis methodology	1287:1343	Results were compared by statistical approaches using multivariate analysis with a partial least squares discriminant analysis methodology.
26280926	5	69	theme	art	709:711	arg1	methodologies					713:725	the art methodologies	705:725	the art methodologies	705:725	In this study, samples from several batches of generic enoxaparins were purchased on the US market and analyzed with state of the art methodologies, including disaccharide building blocks quantification, nuclear magnetic resonance (NMR), and a combination of orthogonal separation techniques.
26280926	0	70	from	comparability	27:39	arg1	market					76:81	the US market	69:81	the US market	69:81	Analytical and statistical comparability of generic enoxaparins from the US market with the originator product.
26280926	5	71	theme	magnetic	791:798	arg1	NMR					811:813	NMR	811:813	NMR	811:813	In this study, samples from several batches of generic enoxaparins were purchased on the US market and analyzed with state of the art methodologies, including disaccharide building blocks quantification, nuclear magnetic resonance (NMR), and a combination of orthogonal separation techniques.
26280926	5	71	theme	magnetic	791:798	arg1	resonance					800:808	nuclear magnetic resonance	783:808	nuclear magnetic resonance (NMR)	783:814	In this study, samples from several batches of generic enoxaparins were purchased on the US market and analyzed with state of the art methodologies, including disaccharide building blocks quantification, nuclear magnetic resonance (NMR), and a combination of orthogonal separation techniques.
26280926	5	72	theme	orthogonal	838:847	arg1	techniques					860:869	orthogonal separation techniques	838:869	orthogonal separation techniques	838:869	In this study, samples from several batches of generic enoxaparins were purchased on the US market and analyzed with state of the art methodologies, including disaccharide building blocks quantification, nuclear magnetic resonance (NMR), and a combination of orthogonal separation techniques.
26280926	9	73	theme	enoxaparin	1447:1456	arg1	batches					1458:1464	the enoxaparin batches	1443:1464	the enoxaparin batches according to their manufacturer	1443:1496	The variations were statistically significant and allowed a clear distinction to be made between the enoxaparin batches according to their manufacturer.
26314273	6	0	theme	MOF	862:864	arg1	crystallites					866:877	the MOF crystallites	858:877	the MOF crystallites	858:877	Ammonia break-through experiments highlight the accessibility of the MOF crystallites and the adsorption potential of the composite indicating their high potential for filtration applications for toxic industrial gases.
26314273	1	1	theme	air-filtration	176:189	arg1	applications					191:202	gas-separation and air-filtration applications	157:202	gas-separation and air-filtration applications	157:202	Metal-organic frameworks (MOFs) are promising materials for gas-separation and air-filtration applications.
26314273	5	2	theme	pore	622:625	arg1	system					627:632	a hierarchical pore system	607:632	a hierarchical pore system	607:632	This composite has a hierarchical pore system with surface areas up to 800 m(2) g(-1) and pore volumes of 3.6 cm(3) g(-1) , allowing good transport kinetics and a very high loading of the active material.
26314273	3	3	theme	non-toxic	379:387	arg1	material					428:435	a non-toxic, biodegradable, and low-weight support material	377:435	a non-toxic, biodegradable, and low-weight support material for MOF deposition	377:454	We used chitin-based networks from a marine sponge as a non-toxic, biodegradable, and low-weight support material for MOF deposition.
26314273	3	3	theme	non-toxic	379:387	arg1	networks					344:351	chitin-based networks	331:351	chitin-based networks from a marine sponge	331:372	We used chitin-based networks from a marine sponge as a non-toxic, biodegradable, and low-weight support material for MOF deposition.
26314273	4	4	theme	crystallites	539:550	arg1	nucleation					517:526	predominant nucleation	505:526	predominant nucleation of the MOF crystallites	505:550	The structural properties of the material favor predominant nucleation of the MOF crystallites at the inside of the hollow fibers.
26314273	6	5	theme	high	942:945	arg1	potential					947:955	their high potential	936:955	their high potential for filtration applications for toxic industrial gases	936:1010	Ammonia break-through experiments highlight the accessibility of the MOF crystallites and the adsorption potential of the composite indicating their high potential for filtration applications for toxic industrial gases.
26314273	4	6	theme	predominant	505:515	arg1	nucleation					517:526	predominant nucleation	505:526	predominant nucleation of the MOF crystallites	505:550	The structural properties of the material favor predominant nucleation of the MOF crystallites at the inside of the hollow fibers.
26314273	0	7	theme	Biological	0:9	arg1	Composites					22:31	Biological Chitin-MOF Composites	0:31	Biological Chitin-MOF Composites with Hierarchical Pore Systems for Air-Filtration Applications	0:94	Biological Chitin-MOF Composites with Hierarchical Pore Systems for Air-Filtration Applications.
26314273	5	8	theme	surface	639:645	arg1	areas					647:651	surface areas	639:651	surface areas up to 800 m(2) g(-1) and pore volumes of 3.6 cm(3) g(-1)	639:708	This composite has a hierarchical pore system with surface areas up to 800 m(2) g(-1) and pore volumes of 3.6 cm(3) g(-1) , allowing good transport kinetics and a very high loading of the active material.
26314273	2	9	theme	support	304:310	arg1	materials					312:320	robust and manageable support materials	282:320	robust and manageable support materials	282:320	However, for these applications, MOF crystallites need to be incorporated in robust and manageable support materials.
26314273	5	10	theme	material	783:790	arg1	loading					761:767	a very high loading	749:767	a very high loading of the active material	749:790	This composite has a hierarchical pore system with surface areas up to 800 m(2) g(-1) and pore volumes of 3.6 cm(3) g(-1) , allowing good transport kinetics and a very high loading of the active material.
26314273	5	10	theme	material	783:790	arg1	kinetics					736:743	good transport kinetics	721:743	good transport kinetics	721:743	This composite has a hierarchical pore system with surface areas up to 800 m(2) g(-1) and pore volumes of 3.6 cm(3) g(-1) , allowing good transport kinetics and a very high loading of the active material.
26314273	6	11	theme	industrial	995:1004	arg1	gases					1006:1010	toxic industrial gases	989:1010	toxic industrial gases	989:1010	Ammonia break-through experiments highlight the accessibility of the MOF crystallites and the adsorption potential of the composite indicating their high potential for filtration applications for toxic industrial gases.
26314273	0	12	theme	Pore	51:54	arg1	Systems					56:62	Hierarchical Pore Systems	38:62	Hierarchical Pore Systems for Air-Filtration Applications	38:94	Biological Chitin-MOF Composites with Hierarchical Pore Systems for Air-Filtration Applications.
26314273	1	13	theme	Metal-organic	97:109	arg1	materials					143:151	promising materials	133:151	promising materials for gas-separation and air-filtration applications	133:202	Metal-organic frameworks (MOFs) are promising materials for gas-separation and air-filtration applications.
26314273	1	13	theme	Metal-organic	97:109	arg1	MOFs					123:126	MOFs	123:126	MOFs	123:126	Metal-organic frameworks (MOFs) are promising materials for gas-separation and air-filtration applications.
26314273	1	13	theme	Metal-organic	97:109	arg1	frameworks					111:120	Metal-organic frameworks	97:120	Metal-organic frameworks (MOFs)	97:127	Metal-organic frameworks (MOFs) are promising materials for gas-separation and air-filtration applications.
26314273	6	14	theme	crystallites	866:877	arg1	potential					898:906	the adsorption potential	883:906	the adsorption potential of the composite indicating their high potential for filtration applications for toxic industrial gases	883:1010	Ammonia break-through experiments highlight the accessibility of the MOF crystallites and the adsorption potential of the composite indicating their high potential for filtration applications for toxic industrial gases.
26314273	6	14	theme	crystallites	866:877	arg1	accessibility					841:853	the accessibility	837:853	the accessibility of the MOF crystallites	837:877	Ammonia break-through experiments highlight the accessibility of the MOF crystallites and the adsorption potential of the composite indicating their high potential for filtration applications for toxic industrial gases.
26314273	0	15	theme	Chitin-MOF	11:20	arg1	Composites					22:31	Biological Chitin-MOF Composites	0:31	Biological Chitin-MOF Composites with Hierarchical Pore Systems for Air-Filtration Applications	0:94	Biological Chitin-MOF Composites with Hierarchical Pore Systems for Air-Filtration Applications.
26314273	3	16	theme	low-weight	409:418	arg1	material					428:435	a non-toxic, biodegradable, and low-weight support material	377:435	a non-toxic, biodegradable, and low-weight support material for MOF deposition	377:454	We used chitin-based networks from a marine sponge as a non-toxic, biodegradable, and low-weight support material for MOF deposition.
26314273	3	16	theme	low-weight	409:418	arg1	networks					344:351	chitin-based networks	331:351	chitin-based networks from a marine sponge	331:372	We used chitin-based networks from a marine sponge as a non-toxic, biodegradable, and low-weight support material for MOF deposition.
26314273	4	17	theme	fibers	580:585	arg1	inside					559:564	the inside	555:564	the inside of the hollow fibers	555:585	The structural properties of the material favor predominant nucleation of the MOF crystallites at the inside of the hollow fibers.
26314273	5	18	theme	800 m	659:663	arg1	g					668:668	800 m(2) g(-1)	659:672	800 m(2) g(-1)	659:672	This composite has a hierarchical pore system with surface areas up to 800 m(2) g(-1) and pore volumes of 3.6 cm(3) g(-1) , allowing good transport kinetics and a very high loading of the active material.
26314273	3	19	theme	biodegradable	390:402	arg1	material					428:435	a non-toxic, biodegradable, and low-weight support material	377:435	a non-toxic, biodegradable, and low-weight support material for MOF deposition	377:454	We used chitin-based networks from a marine sponge as a non-toxic, biodegradable, and low-weight support material for MOF deposition.
26314273	3	19	theme	biodegradable	390:402	arg1	networks					344:351	chitin-based networks	331:351	chitin-based networks from a marine sponge	331:372	We used chitin-based networks from a marine sponge as a non-toxic, biodegradable, and low-weight support material for MOF deposition.
26314273	4	20	theme	hollow	573:578	arg1	fibers					580:585	the hollow fibers	569:585	the hollow fibers	569:585	The structural properties of the material favor predominant nucleation of the MOF crystallites at the inside of the hollow fibers.
26314273	5	21	theme	pore	678:681	arg1	volumes					683:689	pore volumes	678:689	pore volumes of 3.6 cm	678:699	This composite has a hierarchical pore system with surface areas up to 800 m(2) g(-1) and pore volumes of 3.6 cm(3) g(-1) , allowing good transport kinetics and a very high loading of the active material.
26314273	5	22	theme	hierarchical	609:620	arg1	system					627:632	a hierarchical pore system	607:632	a hierarchical pore system	607:632	This composite has a hierarchical pore system with surface areas up to 800 m(2) g(-1) and pore volumes of 3.6 cm(3) g(-1) , allowing good transport kinetics and a very high loading of the active material.
26314273	5	23	theme	transport	726:734	arg1	kinetics					736:743	good transport kinetics	721:743	good transport kinetics	721:743	This composite has a hierarchical pore system with surface areas up to 800 m(2) g(-1) and pore volumes of 3.6 cm(3) g(-1) , allowing good transport kinetics and a very high loading of the active material.
26314273	5	24	theme	high	756:759	arg1	loading					761:767	a very high loading	749:767	a very high loading of the active material	749:790	This composite has a hierarchical pore system with surface areas up to 800 m(2) g(-1) and pore volumes of 3.6 cm(3) g(-1) , allowing good transport kinetics and a very high loading of the active material.
26314273	6	25	theme	break-through	801:813	arg1	experiments					815:825	Ammonia break-through experiments	793:825	Ammonia break-through experiments	793:825	Ammonia break-through experiments highlight the accessibility of the MOF crystallites and the adsorption potential of the composite indicating their high potential for filtration applications for toxic industrial gases.
26314273	3	26	used	used	326:329	arg2	We					323:324	We	323:324	We	323:324	We used chitin-based networks from a marine sponge as a non-toxic, biodegradable, and low-weight support material for MOF deposition.
26314273	6	27	theme	Ammonia	793:799	arg1	experiments					815:825	Ammonia break-through experiments	793:825	Ammonia break-through experiments	793:825	Ammonia break-through experiments highlight the accessibility of the MOF crystallites and the adsorption potential of the composite indicating their high potential for filtration applications for toxic industrial gases.
26314273	5	28	contain	has	603:605	arg1	composite					593:601	This composite	588:601	This composite	588:601	This composite has a hierarchical pore system with surface areas up to 800 m(2) g(-1) and pore volumes of 3.6 cm(3) g(-1) , allowing good transport kinetics and a very high loading of the active material.
26314273	5	28	contain	has	603:605	arg2	system					627:632	a hierarchical pore system	607:632	a hierarchical pore system	607:632	This composite has a hierarchical pore system with surface areas up to 800 m(2) g(-1) and pore volumes of 3.6 cm(3) g(-1) , allowing good transport kinetics and a very high loading of the active material.
26314273	0	29	theme	Hierarchical	38:49	arg1	Systems					56:62	Hierarchical Pore Systems	38:62	Hierarchical Pore Systems for Air-Filtration Applications	38:94	Biological Chitin-MOF Composites with Hierarchical Pore Systems for Air-Filtration Applications.
26314273	3	30	theme	MOF	441:443	arg1	deposition					445:454	MOF deposition	441:454	MOF deposition	441:454	We used chitin-based networks from a marine sponge as a non-toxic, biodegradable, and low-weight support material for MOF deposition.
26314273	4	31	theme	material	490:497	arg1	properties					472:481	The structural properties	457:481	The structural properties of the material	457:497	The structural properties of the material favor predominant nucleation of the MOF crystallites at the inside of the hollow fibers.
26314273	5	32	dep	g	668:668	arg1	2					665:665	2	665:665	2	665:665	This composite has a hierarchical pore system with surface areas up to 800 m(2) g(-1) and pore volumes of 3.6 cm(3) g(-1) , allowing good transport kinetics and a very high loading of the active material.
26314273	5	32	dep	g	668:668	arg1	g					704:704	(3) g(-1)	700:708	(3) g(-1)	700:708	This composite has a hierarchical pore system with surface areas up to 800 m(2) g(-1) and pore volumes of 3.6 cm(3) g(-1) , allowing good transport kinetics and a very high loading of the active material.
26314273	5	32	dep	g	668:668	arg1	up					653:654	up	653:654	up	653:654	This composite has a hierarchical pore system with surface areas up to 800 m(2) g(-1) and pore volumes of 3.6 cm(3) g(-1) , allowing good transport kinetics and a very high loading of the active material.
26314273	5	33	theme	active	776:781	arg1	material					783:790	the active material	772:790	the active material	772:790	This composite has a hierarchical pore system with surface areas up to 800 m(2) g(-1) and pore volumes of 3.6 cm(3) g(-1) , allowing good transport kinetics and a very high loading of the active material.
26314273	3	34	theme	support	420:426	arg1	material					428:435	a non-toxic, biodegradable, and low-weight support material	377:435	a non-toxic, biodegradable, and low-weight support material for MOF deposition	377:454	We used chitin-based networks from a marine sponge as a non-toxic, biodegradable, and low-weight support material for MOF deposition.
26314273	3	34	theme	support	420:426	arg1	networks					344:351	chitin-based networks	331:351	chitin-based networks from a marine sponge	331:372	We used chitin-based networks from a marine sponge as a non-toxic, biodegradable, and low-weight support material for MOF deposition.
26314273	6	35	theme	adsorption	887:896	arg1	potential					898:906	the adsorption potential	883:906	the adsorption potential of the composite indicating their high potential for filtration applications for toxic industrial gases	883:1010	Ammonia break-through experiments highlight the accessibility of the MOF crystallites and the adsorption potential of the composite indicating their high potential for filtration applications for toxic industrial gases.
26314273	3	36	theme	chitin-based	331:342	arg1	material					428:435	a non-toxic, biodegradable, and low-weight support material	377:435	a non-toxic, biodegradable, and low-weight support material for MOF deposition	377:454	We used chitin-based networks from a marine sponge as a non-toxic, biodegradable, and low-weight support material for MOF deposition.
26314273	3	36	theme	chitin-based	331:342	arg1	networks					344:351	chitin-based networks	331:351	chitin-based networks from a marine sponge	331:372	We used chitin-based networks from a marine sponge as a non-toxic, biodegradable, and low-weight support material for MOF deposition.
26314273	6	37	theme	composite	915:923	arg1	potential					898:906	the adsorption potential	883:906	the adsorption potential of the composite indicating their high potential for filtration applications for toxic industrial gases	883:1010	Ammonia break-through experiments highlight the accessibility of the MOF crystallites and the adsorption potential of the composite indicating their high potential for filtration applications for toxic industrial gases.
26314273	6	37	theme	composite	915:923	arg1	accessibility					841:853	the accessibility	837:853	the accessibility of the MOF crystallites	837:877	Ammonia break-through experiments highlight the accessibility of the MOF crystallites and the adsorption potential of the composite indicating their high potential for filtration applications for toxic industrial gases.
26314273	0	38	with	Composites	22:31	arg1	Systems					56:62	Hierarchical Pore Systems	38:62	Hierarchical Pore Systems for Air-Filtration Applications	38:94	Biological Chitin-MOF Composites with Hierarchical Pore Systems for Air-Filtration Applications.
26314273	5	39	theme	3.6 cm	694:699	arg1	volumes					683:689	pore volumes	678:689	pore volumes of 3.6 cm	678:699	This composite has a hierarchical pore system with surface areas up to 800 m(2) g(-1) and pore volumes of 3.6 cm(3) g(-1) , allowing good transport kinetics and a very high loading of the active material.
26314273	5	39	theme	3.6 cm	694:699	arg1	g					668:668	800 m(2) g(-1)	659:672	800 m(2) g(-1)	659:672	This composite has a hierarchical pore system with surface areas up to 800 m(2) g(-1) and pore volumes of 3.6 cm(3) g(-1) , allowing good transport kinetics and a very high loading of the active material.
26314273	5	40	dep	g	704:704	arg1	3					701:701	3	701:701	3	701:701	This composite has a hierarchical pore system with surface areas up to 800 m(2) g(-1) and pore volumes of 3.6 cm(3) g(-1) , allowing good transport kinetics and a very high loading of the active material.
26314273	2	41	theme	MOF	238:240	arg1	crystallites					242:253	MOF crystallites	238:253	MOF crystallites	238:253	However, for these applications, MOF crystallites need to be incorporated in robust and manageable support materials.
26314273	1	42	theme	promising	133:141	arg1	materials					143:151	promising materials	133:151	promising materials for gas-separation and air-filtration applications	133:202	Metal-organic frameworks (MOFs) are promising materials for gas-separation and air-filtration applications.
26314273	1	42	theme	promising	133:141	arg1	frameworks					111:120	Metal-organic frameworks	97:120	Metal-organic frameworks (MOFs)	97:127	Metal-organic frameworks (MOFs) are promising materials for gas-separation and air-filtration applications.
26314273	6	43	theme	toxic	989:993	arg1	gases					1006:1010	toxic industrial gases	989:1010	toxic industrial gases	989:1010	Ammonia break-through experiments highlight the accessibility of the MOF crystallites and the adsorption potential of the composite indicating their high potential for filtration applications for toxic industrial gases.
26314273	2	44	theme	manageable	293:302	arg1	materials					312:320	robust and manageable support materials	282:320	robust and manageable support materials	282:320	However, for these applications, MOF crystallites need to be incorporated in robust and manageable support materials.
26314273	4	45	theme	structural	461:470	arg1	properties					472:481	The structural properties	457:481	The structural properties of the material	457:497	The structural properties of the material favor predominant nucleation of the MOF crystallites at the inside of the hollow fibers.
26314273	2	46	theme	robust	282:287	arg1	materials					312:320	robust and manageable support materials	282:320	robust and manageable support materials	282:320	However, for these applications, MOF crystallites need to be incorporated in robust and manageable support materials.
26314273	3	47	theme	marine	360:365	arg1	sponge					367:372	a marine sponge	358:372	a marine sponge	358:372	We used chitin-based networks from a marine sponge as a non-toxic, biodegradable, and low-weight support material for MOF deposition.
26314273	3	48	from	sponge	367:372	arg1	material					428:435	a non-toxic, biodegradable, and low-weight support material	377:435	a non-toxic, biodegradable, and low-weight support material for MOF deposition	377:454	We used chitin-based networks from a marine sponge as a non-toxic, biodegradable, and low-weight support material for MOF deposition.
26314273	3	48	from	sponge	367:372	arg1	networks					344:351	chitin-based networks	331:351	chitin-based networks from a marine sponge	331:372	We used chitin-based networks from a marine sponge as a non-toxic, biodegradable, and low-weight support material for MOF deposition.
26314273	1	49	theme	gas-separation	157:170	arg1	applications					191:202	gas-separation and air-filtration applications	157:202	gas-separation and air-filtration applications	157:202	Metal-organic frameworks (MOFs) are promising materials for gas-separation and air-filtration applications.
26314273	0	50	theme	Air-Filtration	68:81	arg1	Applications					83:94	Air-Filtration Applications	68:94	Air-Filtration Applications	68:94	Biological Chitin-MOF Composites with Hierarchical Pore Systems for Air-Filtration Applications.
26314273	4	51	theme	MOF	535:537	arg1	crystallites					539:550	the MOF crystallites	531:550	the MOF crystallites	531:550	The structural properties of the material favor predominant nucleation of the MOF crystallites at the inside of the hollow fibers.
26314273	5	52	theme	good	721:724	arg1	kinetics					736:743	good transport kinetics	721:743	good transport kinetics	721:743	This composite has a hierarchical pore system with surface areas up to 800 m(2) g(-1) and pore volumes of 3.6 cm(3) g(-1) , allowing good transport kinetics and a very high loading of the active material.
26314273	6	53	theme	filtration	961:970	arg1	applications					972:983	filtration applications	961:983	filtration applications	961:983	Ammonia break-through experiments highlight the accessibility of the MOF crystallites and the adsorption potential of the composite indicating their high potential for filtration applications for toxic industrial gases.
25272753	2	0	theme	previous	108:115	arg1	study					117:121	our previous study	104:121	our previous study	104:121	In our previous study we reported on the bioflocculant production by a Bacillus species isolated from sediment samples of Algoa Bay in the Eastern Cape Province of South Africa.
25272753	3	1	contain	carried	299:305	arg3	study					290:294	current study	282:294	current study	282:294	In current study we carried out further evaluation on the effect of different culture conditions on the bioflocculant production, as well as characterised the bioflocculant produced in detail.
25272753	3	1	contain	carried	299:305	arg3	effect					337:342	the effect	333:342	the effect of different culture conditions on the bioflocculant production, as well as characterised the bioflocculant produced in detail	333:469	In current study we carried out further evaluation on the effect of different culture conditions on the bioflocculant production, as well as characterised the bioflocculant produced in detail.
25272753	3	1	contain	carried	299:305	arg2	evaluation					319:328	further evaluation	311:328	further evaluation	311:328	In current study we carried out further evaluation on the effect of different culture conditions on the bioflocculant production, as well as characterised the bioflocculant produced in detail.
25272753	3	1	contain	carried	299:305	arg1	we					296:297	we	296:297	we	296:297	In current study we carried out further evaluation on the effect of different culture conditions on the bioflocculant production, as well as characterised the bioflocculant produced in detail.
25272753	4	2	theme	nitrogen	669:676	arg1	carbonate					563:571	sodium carbonate	556:571	sodium carbonate (95.2% flocculating activity)	556:601	The bacteria produced bioflocculant optimally under the following conditions: using sodium carbonate (95.2% flocculating activity) and potassium nitrate (76.6% flocculating activity) as carbon and nitrogen sources, respectively; inoculum size of 3% (v/v); initial pH 9.0; and Al3+ as coagulant aid.
25272753	4	2	theme	nitrogen	669:676	arg1	nitrate					617:623	potassium nitrate	607:623	potassium nitrate (76.6% flocculating activity)	607:653	The bacteria produced bioflocculant optimally under the following conditions: using sodium carbonate (95.2% flocculating activity) and potassium nitrate (76.6% flocculating activity) as carbon and nitrogen sources, respectively; inoculum size of 3% (v/v); initial pH 9.0; and Al3+ as coagulant aid.
25272753	4	2	theme	nitrogen	669:676	arg1	sources					678:684	carbon and nitrogen sources	658:684	sources	678:684	The bacteria produced bioflocculant optimally under the following conditions: using sodium carbonate (95.2% flocculating activity) and potassium nitrate (76.6% flocculating activity) as carbon and nitrogen sources, respectively; inoculum size of 3% (v/v); initial pH 9.0; and Al3+ as coagulant aid.
25272753	1	3	from	environment	72:82	arg1	Africa					93:98	Africa	93:98	Africa	93:98	Gilbert isolated from a marine environment in South Africa.
25272753	6	4	theme	sp	918:919	arg1	analysis					893:900	Chemical analysis	884:900	Chemical analysis of the Bacillus sp	884:919	Chemical analysis of the Bacillus sp.
25272753	8	5	theme	hydroxyl	1087:1094	arg1	groups					1120:1125	hydroxyl, carboxyl and methylene groups	1087:1125	groups	1120:1125	Fourier transform infrared spectroscopy analysis revealed the presence of hydroxyl, carboxyl and methylene groups in the bioflocculant and energy-dispersive X-ray analysis detected the elemental composition in mass proportion (% w/w) of C, N, O, S and P as 4.12 : 7.40: 39.92: 3.00: 13.91.
25272753	5	6	theme	flocculating	819:830	arg1	activity					832:839	44.2% residual flocculating activity	804:839	44.2% residual flocculating activity	804:839	The crude bioflocculant retained 44.2% residual flocculating activity after heating at 100 degrees C for 15 min.
25272753	8	7	from	presence	1075:1082	arg1	bioflocculant					1134:1146	the bioflocculant	1130:1146	the bioflocculant	1130:1146	Fourier transform infrared spectroscopy analysis revealed the presence of hydroxyl, carboxyl and methylene groups in the bioflocculant and energy-dispersive X-ray analysis detected the elemental composition in mass proportion (% w/w) of C, N, O, S and P as 4.12 : 7.40: 39.92: 3.00: 13.91.
25272753	8	7	from	presence	1075:1082	arg1	analysis					1176:1183	energy-dispersive X-ray analysis	1152:1183	energy-dispersive X-ray analysis	1152:1183	Fourier transform infrared spectroscopy analysis revealed the presence of hydroxyl, carboxyl and methylene groups in the bioflocculant and energy-dispersive X-ray analysis detected the elemental composition in mass proportion (% w/w) of C, N, O, S and P as 4.12 : 7.40: 39.92: 3.00: 13.91.
25272753	6	8	theme	Bacillus	909:916	arg1	sp					918:919	the Bacillus sp	905:919	the Bacillus sp	905:919	Chemical analysis of the Bacillus sp.
25272753	3	9	theme	culture	357:363	arg1	conditions					365:374	different culture conditions	347:374	different culture conditions	347:374	In current study we carried out further evaluation on the effect of different culture conditions on the bioflocculant production, as well as characterised the bioflocculant produced in detail.
25272753	8	10	attach	presence	1075:1082	arg2	groups					1120:1125	hydroxyl, carboxyl and methylene groups	1087:1125	groups	1120:1125	Fourier transform infrared spectroscopy analysis revealed the presence of hydroxyl, carboxyl and methylene groups in the bioflocculant and energy-dispersive X-ray analysis detected the elemental composition in mass proportion (% w/w) of C, N, O, S and P as 4.12 : 7.40: 39.92: 3.00: 13.91.
25272753	8	10	attach	presence	1075:1082	arg1	bioflocculant					1134:1146	the bioflocculant	1130:1146	the bioflocculant	1130:1146	Fourier transform infrared spectroscopy analysis revealed the presence of hydroxyl, carboxyl and methylene groups in the bioflocculant and energy-dispersive X-ray analysis detected the elemental composition in mass proportion (% w/w) of C, N, O, S and P as 4.12 : 7.40: 39.92: 3.00: 13.91.
25272753	8	10	attach	presence	1075:1082	arg1	analysis					1176:1183	energy-dispersive X-ray analysis	1152:1183	energy-dispersive X-ray analysis	1152:1183	Fourier transform infrared spectroscopy analysis revealed the presence of hydroxyl, carboxyl and methylene groups in the bioflocculant and energy-dispersive X-ray analysis detected the elemental composition in mass proportion (% w/w) of C, N, O, S and P as 4.12 : 7.40: 39.92: 3.00: 13.91.
25272753	4	11	theme	flocculating	632:643	arg1	activity					645:652	76.6% flocculating activity	626:652	76.6% flocculating activity	626:652	The bacteria produced bioflocculant optimally under the following conditions: using sodium carbonate (95.2% flocculating activity) and potassium nitrate (76.6% flocculating activity) as carbon and nitrogen sources, respectively; inoculum size of 3% (v/v); initial pH 9.0; and Al3+ as coagulant aid.
25272753	4	11	theme	flocculating	632:643	arg1	nitrate					617:623	potassium nitrate	607:623	potassium nitrate (76.6% flocculating activity)	607:653	The bacteria produced bioflocculant optimally under the following conditions: using sodium carbonate (95.2% flocculating activity) and potassium nitrate (76.6% flocculating activity) as carbon and nitrogen sources, respectively; inoculum size of 3% (v/v); initial pH 9.0; and Al3+ as coagulant aid.
25272753	4	12	theme	%	578:578	arg1	activity					593:600	95.2% flocculating activity	574:600	95.2% flocculating activity	574:600	The bacteria produced bioflocculant optimally under the following conditions: using sodium carbonate (95.2% flocculating activity) and potassium nitrate (76.6% flocculating activity) as carbon and nitrogen sources, respectively; inoculum size of 3% (v/v); initial pH 9.0; and Al3+ as coagulant aid.
25272753	4	12	theme	%	578:578	arg1	carbonate					563:571	sodium carbonate	556:571	sodium carbonate (95.2% flocculating activity)	556:601	The bacteria produced bioflocculant optimally under the following conditions: using sodium carbonate (95.2% flocculating activity) and potassium nitrate (76.6% flocculating activity) as carbon and nitrogen sources, respectively; inoculum size of 3% (v/v); initial pH 9.0; and Al3+ as coagulant aid.
25272753	8	13	theme	carboxyl	1097:1104	arg1	groups					1120:1125	hydroxyl, carboxyl and methylene groups	1087:1125	groups	1120:1125	Fourier transform infrared spectroscopy analysis revealed the presence of hydroxyl, carboxyl and methylene groups in the bioflocculant and energy-dispersive X-ray analysis detected the elemental composition in mass proportion (% w/w) of C, N, O, S and P as 4.12 : 7.40: 39.92: 3.00: 13.91.
25272753	3	14	from	effect	337:342	arg1	production					397:406	the bioflocculant production	379:406	the bioflocculant production	379:406	In current study we carried out further evaluation on the effect of different culture conditions on the bioflocculant production, as well as characterised the bioflocculant produced in detail.
25272753	4	15	theme	%	719:719	arg1	size					710:713	inoculum size	701:713	inoculum size of 3% (v/v); initial pH 9.0; and Al3+ as coagulant aid	701:768	The bacteria produced bioflocculant optimally under the following conditions: using sodium carbonate (95.2% flocculating activity) and potassium nitrate (76.6% flocculating activity) as carbon and nitrogen sources, respectively; inoculum size of 3% (v/v); initial pH 9.0; and Al3+ as coagulant aid.
25272753	9	16	theme	Scanning	1303:1310	arg1	image					1332:1336	Scanning electron micrograph image	1303:1336	Scanning electron micrograph image of the bioflocculant	1303:1357	Scanning electron micrograph image of the bioflocculant revealed an amorphous compound.
25272753	5	17	theme	crude	775:779	arg1	bioflocculant					781:793	The crude bioflocculant	771:793	The crude bioflocculant	771:793	The crude bioflocculant retained 44.2% residual flocculating activity after heating at 100 degrees C for 15 min.
25272753	3	18	theme	further	311:317	arg1	evaluation					319:328	further evaluation	311:328	further evaluation	311:328	In current study we carried out further evaluation on the effect of different culture conditions on the bioflocculant production, as well as characterised the bioflocculant produced in detail.
25272753	8	19	theme	groups	1120:1125	arg1	presence					1075:1082	the presence	1071:1082	the presence of hydroxyl, carboxyl and methylene groups in the bioflocculant and energy-dispersive X-ray analysis detected the elemental composition in mass proportion (% w/w) of C, N, O, S and P as 4.12 : 7.40: 39.92: 3.00: 13.91	1071:1300	Fourier transform infrared spectroscopy analysis revealed the presence of hydroxyl, carboxyl and methylene groups in the bioflocculant and energy-dispersive X-ray analysis detected the elemental composition in mass proportion (% w/w) of C, N, O, S and P as 4.12 : 7.40: 39.92: 3.00: 13.91.
25272753	8	20	theme	mass	1223:1226	arg1	proportion					1228:1237	mass proportion	1223:1237	mass proportion (% w/w) of C, N, O, S and P	1223:1265	Fourier transform infrared spectroscopy analysis revealed the presence of hydroxyl, carboxyl and methylene groups in the bioflocculant and energy-dispersive X-ray analysis detected the elemental composition in mass proportion (% w/w) of C, N, O, S and P as 4.12 : 7.40: 39.92: 3.00: 13.91.
25272753	8	20	theme	mass	1223:1226	arg1	w/w					1242:1244	% w/w	1240:1244	% w/w	1240:1244	Fourier transform infrared spectroscopy analysis revealed the presence of hydroxyl, carboxyl and methylene groups in the bioflocculant and energy-dispersive X-ray analysis detected the elemental composition in mass proportion (% w/w) of C, N, O, S and P as 4.12 : 7.40: 39.92: 3.00: 13.91.
25272753	1	21	theme	marine	65:70	arg1	environment					72:82	a marine environment	63:82	a marine environment in South Africa	63:98	Gilbert isolated from a marine environment in South Africa.
25272753	2	22	attach	isolated	189:196	arg2	species					181:187	a Bacillus species	170:187	a Bacillus species isolated from sediment samples of Algoa Bay in the Eastern Cape Province of South Africa	170:276	In our previous study we reported on the bioflocculant production by a Bacillus species isolated from sediment samples of Algoa Bay in the Eastern Cape Province of South Africa.
25272753	2	22	attach	isolated	189:196	arg1	samples					212:218	sediment samples	203:218	sediment samples of Algoa Bay	203:231	In our previous study we reported on the bioflocculant production by a Bacillus species isolated from sediment samples of Algoa Bay in the Eastern Cape Province of South Africa.
25272753	8	23	theme	methylene	1110:1118	arg1	groups					1120:1125	hydroxyl, carboxyl and methylene groups	1087:1125	groups	1120:1125	Fourier transform infrared spectroscopy analysis revealed the presence of hydroxyl, carboxyl and methylene groups in the bioflocculant and energy-dispersive X-ray analysis detected the elemental composition in mass proportion (% w/w) of C, N, O, S and P as 4.12 : 7.40: 39.92: 3.00: 13.91.
25272753	2	24	theme	Cape	248:251	arg1	Province					253:260	the Eastern Cape Province	236:260	the Eastern Cape Province of South Africa	236:276	In our previous study we reported on the bioflocculant production by a Bacillus species isolated from sediment samples of Algoa Bay in the Eastern Cape Province of South Africa.
25272753	4	25	theme	%	630:630	arg1	activity					645:652	76.6% flocculating activity	626:652	76.6% flocculating activity	626:652	The bacteria produced bioflocculant optimally under the following conditions: using sodium carbonate (95.2% flocculating activity) and potassium nitrate (76.6% flocculating activity) as carbon and nitrogen sources, respectively; inoculum size of 3% (v/v); initial pH 9.0; and Al3+ as coagulant aid.
25272753	4	25	theme	%	630:630	arg1	nitrate					617:623	potassium nitrate	607:623	potassium nitrate (76.6% flocculating activity)	607:653	The bacteria produced bioflocculant optimally under the following conditions: using sodium carbonate (95.2% flocculating activity) and potassium nitrate (76.6% flocculating activity) as carbon and nitrogen sources, respectively; inoculum size of 3% (v/v); initial pH 9.0; and Al3+ as coagulant aid.
25272753	3	26	theme	different	347:355	arg1	conditions					365:374	different culture conditions	347:374	different culture conditions	347:374	In current study we carried out further evaluation on the effect of different culture conditions on the bioflocculant production, as well as characterised the bioflocculant produced in detail.
25272753	5	27	theme	degrees	862:868	arg1	C					870:870	100 degrees C	858:870	100 degrees C	858:870	The crude bioflocculant retained 44.2% residual flocculating activity after heating at 100 degrees C for 15 min.
25272753	8	28	theme	spectroscopy	1040:1051	arg1	analysis					1053:1060	infrared spectroscopy analysis	1031:1060	infrared spectroscopy analysis	1031:1060	Fourier transform infrared spectroscopy analysis revealed the presence of hydroxyl, carboxyl and methylene groups in the bioflocculant and energy-dispersive X-ray analysis detected the elemental composition in mass proportion (% w/w) of C, N, O, S and P as 4.12 : 7.40: 39.92: 3.00: 13.91.
25272753	8	29	dep	4.12	1270:1273	arg1	13.91					1296:1300	13.91	1296:1300	13.91	1296:1300	Fourier transform infrared spectroscopy analysis revealed the presence of hydroxyl, carboxyl and methylene groups in the bioflocculant and energy-dispersive X-ray analysis detected the elemental composition in mass proportion (% w/w) of C, N, O, S and P as 4.12 : 7.40: 39.92: 3.00: 13.91.
25272753	8	29	dep	4.12	1270:1273	arg1	3.00					1290:1293	3.00	1290:1293	3.00	1290:1293	Fourier transform infrared spectroscopy analysis revealed the presence of hydroxyl, carboxyl and methylene groups in the bioflocculant and energy-dispersive X-ray analysis detected the elemental composition in mass proportion (% w/w) of C, N, O, S and P as 4.12 : 7.40: 39.92: 3.00: 13.91.
25272753	8	29	dep	4.12	1270:1273	arg1	39.92					1283:1287	39.92	1283:1287	39.92	1283:1287	Fourier transform infrared spectroscopy analysis revealed the presence of hydroxyl, carboxyl and methylene groups in the bioflocculant and energy-dispersive X-ray analysis detected the elemental composition in mass proportion (% w/w) of C, N, O, S and P as 4.12 : 7.40: 39.92: 3.00: 13.91.
25272753	8	29	dep	4.12	1270:1273	arg1	7.40					1277:1280	7.40	1277:1280	7.40	1277:1280	Fourier transform infrared spectroscopy analysis revealed the presence of hydroxyl, carboxyl and methylene groups in the bioflocculant and energy-dispersive X-ray analysis detected the elemental composition in mass proportion (% w/w) of C, N, O, S and P as 4.12 : 7.40: 39.92: 3.00: 13.91.
25272753	0	30	theme	Bioflocculant	0:12	arg1	production					14:23	Bioflocculant production	0:23	Bioflocculant production by Bacillus sp	0:38	Bioflocculant production by Bacillus sp.
25272753	8	31	theme	elemental	1198:1206	arg1	composition					1208:1218	the elemental composition	1194:1218	the elemental composition in mass proportion (% w/w) of C, N, O, S and P	1194:1265	Fourier transform infrared spectroscopy analysis revealed the presence of hydroxyl, carboxyl and methylene groups in the bioflocculant and energy-dispersive X-ray analysis detected the elemental composition in mass proportion (% w/w) of C, N, O, S and P as 4.12 : 7.40: 39.92: 3.00: 13.91.
25272753	4	32	theme	inoculum	701:708	arg1	size					710:713	inoculum size	701:713	inoculum size of 3% (v/v); initial pH 9.0; and Al3+ as coagulant aid	701:768	The bacteria produced bioflocculant optimally under the following conditions: using sodium carbonate (95.2% flocculating activity) and potassium nitrate (76.6% flocculating activity) as carbon and nitrogen sources, respectively; inoculum size of 3% (v/v); initial pH 9.0; and Al3+ as coagulant aid.
25272753	5	33	theme	44.2	804:807	arg1	%					808:808	%	808:808	%	808:808	The crude bioflocculant retained 44.2% residual flocculating activity after heating at 100 degrees C for 15 min.
25272753	0	34	theme	Bacillus	28:35	arg1	sp					37:38	Bacillus sp	28:38	Bacillus sp	28:38	Bioflocculant production by Bacillus sp.
25272753	6	35	theme	Chemical	884:891	arg1	analysis					893:900	Chemical analysis	884:900	Chemical analysis of the Bacillus sp	884:919	Chemical analysis of the Bacillus sp.
25272753	4	36	theme	Al3+	748:751	arg1	size					710:713	inoculum size	701:713	inoculum size of 3% (v/v); initial pH 9.0; and Al3+ as coagulant aid	701:768	The bacteria produced bioflocculant optimally under the following conditions: using sodium carbonate (95.2% flocculating activity) and potassium nitrate (76.6% flocculating activity) as carbon and nitrogen sources, respectively; inoculum size of 3% (v/v); initial pH 9.0; and Al3+ as coagulant aid.
25272753	9	37	theme	electron	1312:1319	arg1	image					1332:1336	Scanning electron micrograph image	1303:1336	Scanning electron micrograph image of the bioflocculant	1303:1357	Scanning electron micrograph image of the bioflocculant revealed an amorphous compound.
25272753	9	38	theme	bioflocculant	1345:1357	arg1	image					1332:1336	Scanning electron micrograph image	1303:1336	Scanning electron micrograph image of the bioflocculant	1303:1357	Scanning electron micrograph image of the bioflocculant revealed an amorphous compound.
25272753	4	39	dep	coagulant	756:764	arg1	aid					766:768	aid	766:768	aid	766:768	The bacteria produced bioflocculant optimally under the following conditions: using sodium carbonate (95.2% flocculating activity) and potassium nitrate (76.6% flocculating activity) as carbon and nitrogen sources, respectively; inoculum size of 3% (v/v); initial pH 9.0; and Al3+ as coagulant aid.
25272753	4	40	theme	sodium	556:561	arg1	activity					593:600	95.2% flocculating activity	574:600	95.2% flocculating activity	574:600	The bacteria produced bioflocculant optimally under the following conditions: using sodium carbonate (95.2% flocculating activity) and potassium nitrate (76.6% flocculating activity) as carbon and nitrogen sources, respectively; inoculum size of 3% (v/v); initial pH 9.0; and Al3+ as coagulant aid.
25272753	4	40	theme	sodium	556:561	arg1	carbonate					563:571	sodium carbonate	556:571	sodium carbonate (95.2% flocculating activity)	556:601	The bacteria produced bioflocculant optimally under the following conditions: using sodium carbonate (95.2% flocculating activity) and potassium nitrate (76.6% flocculating activity) as carbon and nitrogen sources, respectively; inoculum size of 3% (v/v); initial pH 9.0; and Al3+ as coagulant aid.
25272753	4	40	theme	sodium	556:561	arg1	nitrate					617:623	potassium nitrate	607:623	potassium nitrate (76.6% flocculating activity)	607:653	The bacteria produced bioflocculant optimally under the following conditions: using sodium carbonate (95.2% flocculating activity) and potassium nitrate (76.6% flocculating activity) as carbon and nitrogen sources, respectively; inoculum size of 3% (v/v); initial pH 9.0; and Al3+ as coagulant aid.
25272753	4	40	theme	sodium	556:561	arg1	sources					678:684	carbon and nitrogen sources	658:684	sources	678:684	The bacteria produced bioflocculant optimally under the following conditions: using sodium carbonate (95.2% flocculating activity) and potassium nitrate (76.6% flocculating activity) as carbon and nitrogen sources, respectively; inoculum size of 3% (v/v); initial pH 9.0; and Al3+ as coagulant aid.
25272753	8	41	theme	P	1265:1265	arg1	proportion					1228:1237	mass proportion	1223:1237	mass proportion (% w/w) of C, N, O, S and P	1223:1265	Fourier transform infrared spectroscopy analysis revealed the presence of hydroxyl, carboxyl and methylene groups in the bioflocculant and energy-dispersive X-ray analysis detected the elemental composition in mass proportion (% w/w) of C, N, O, S and P as 4.12 : 7.40: 39.92: 3.00: 13.91.
25272753	8	41	theme	P	1265:1265	arg1	w/w					1242:1244	% w/w	1240:1244	% w/w	1240:1244	Fourier transform infrared spectroscopy analysis revealed the presence of hydroxyl, carboxyl and methylene groups in the bioflocculant and energy-dispersive X-ray analysis detected the elemental composition in mass proportion (% w/w) of C, N, O, S and P as 4.12 : 7.40: 39.92: 3.00: 13.91.
25272753	2	42	theme	Africa	271:276	arg1	Province					253:260	the Eastern Cape Province	236:260	the Eastern Cape Province of South Africa	236:276	In our previous study we reported on the bioflocculant production by a Bacillus species isolated from sediment samples of Algoa Bay in the Eastern Cape Province of South Africa.
25272753	5	43	theme	%	808:808	arg1	activity					832:839	44.2% residual flocculating activity	804:839	44.2% residual flocculating activity	804:839	The crude bioflocculant retained 44.2% residual flocculating activity after heating at 100 degrees C for 15 min.
25272753	4	44	theme	potassium	607:615	arg1	carbonate					563:571	sodium carbonate	556:571	sodium carbonate (95.2% flocculating activity)	556:601	The bacteria produced bioflocculant optimally under the following conditions: using sodium carbonate (95.2% flocculating activity) and potassium nitrate (76.6% flocculating activity) as carbon and nitrogen sources, respectively; inoculum size of 3% (v/v); initial pH 9.0; and Al3+ as coagulant aid.
25272753	4	44	theme	potassium	607:615	arg1	activity					645:652	76.6% flocculating activity	626:652	76.6% flocculating activity	626:652	The bacteria produced bioflocculant optimally under the following conditions: using sodium carbonate (95.2% flocculating activity) and potassium nitrate (76.6% flocculating activity) as carbon and nitrogen sources, respectively; inoculum size of 3% (v/v); initial pH 9.0; and Al3+ as coagulant aid.
25272753	4	44	theme	potassium	607:615	arg1	nitrate					617:623	potassium nitrate	607:623	potassium nitrate (76.6% flocculating activity)	607:653	The bacteria produced bioflocculant optimally under the following conditions: using sodium carbonate (95.2% flocculating activity) and potassium nitrate (76.6% flocculating activity) as carbon and nitrogen sources, respectively; inoculum size of 3% (v/v); initial pH 9.0; and Al3+ as coagulant aid.
25272753	4	44	theme	potassium	607:615	arg1	sources					678:684	carbon and nitrogen sources	658:684	sources	678:684	The bacteria produced bioflocculant optimally under the following conditions: using sodium carbonate (95.2% flocculating activity) and potassium nitrate (76.6% flocculating activity) as carbon and nitrogen sources, respectively; inoculum size of 3% (v/v); initial pH 9.0; and Al3+ as coagulant aid.
25272753	8	45	theme	X-ray	1170:1174	arg1	analysis					1176:1183	energy-dispersive X-ray analysis	1152:1183	energy-dispersive X-ray analysis	1152:1183	Fourier transform infrared spectroscopy analysis revealed the presence of hydroxyl, carboxyl and methylene groups in the bioflocculant and energy-dispersive X-ray analysis detected the elemental composition in mass proportion (% w/w) of C, N, O, S and P as 4.12 : 7.40: 39.92: 3.00: 13.91.
25272753	8	46	theme	infrared	1031:1038	arg1	analysis					1053:1060	infrared spectroscopy analysis	1031:1060	infrared spectroscopy analysis	1031:1060	Fourier transform infrared spectroscopy analysis revealed the presence of hydroxyl, carboxyl and methylene groups in the bioflocculant and energy-dispersive X-ray analysis detected the elemental composition in mass proportion (% w/w) of C, N, O, S and P as 4.12 : 7.40: 39.92: 3.00: 13.91.
25272753	8	47	theme	%	1240:1240	arg1	proportion					1228:1237	mass proportion	1223:1237	mass proportion (% w/w) of C, N, O, S and P	1223:1265	Fourier transform infrared spectroscopy analysis revealed the presence of hydroxyl, carboxyl and methylene groups in the bioflocculant and energy-dispersive X-ray analysis detected the elemental composition in mass proportion (% w/w) of C, N, O, S and P as 4.12 : 7.40: 39.92: 3.00: 13.91.
25272753	8	47	theme	%	1240:1240	arg1	w/w					1242:1244	% w/w	1240:1244	% w/w	1240:1244	Fourier transform infrared spectroscopy analysis revealed the presence of hydroxyl, carboxyl and methylene groups in the bioflocculant and energy-dispersive X-ray analysis detected the elemental composition in mass proportion (% w/w) of C, N, O, S and P as 4.12 : 7.40: 39.92: 3.00: 13.91.
25272753	3	48	theme	bioflocculant	383:395	arg1	production					397:406	the bioflocculant production	379:406	the bioflocculant production	379:406	In current study we carried out further evaluation on the effect of different culture conditions on the bioflocculant production, as well as characterised the bioflocculant produced in detail.
25272753	8	49	theme	energy-dispersive	1152:1168	arg1	analysis					1176:1183	energy-dispersive X-ray analysis	1152:1183	energy-dispersive X-ray analysis	1152:1183	Fourier transform infrared spectroscopy analysis revealed the presence of hydroxyl, carboxyl and methylene groups in the bioflocculant and energy-dispersive X-ray analysis detected the elemental composition in mass proportion (% w/w) of C, N, O, S and P as 4.12 : 7.40: 39.92: 3.00: 13.91.
25272753	7	50	theme	purified	930:937	arg1	bioflocculant					939:951	Gilbert purified bioflocculant	922:951	Gilbert purified bioflocculant	922:951	Gilbert purified bioflocculant demonstrated that it was composed mainly of polysaccharide.
25272753	3	51	theme	conditions	365:374	arg1	effect					337:342	the effect	333:342	the effect of different culture conditions on the bioflocculant production, as well as characterised the bioflocculant produced in detail	333:469	In current study we carried out further evaluation on the effect of different culture conditions on the bioflocculant production, as well as characterised the bioflocculant produced in detail.
25272753	4	52	dep	produced	485:492	arg1	size					710:713	inoculum size	701:713	inoculum size of 3% (v/v); initial pH 9.0; and Al3+ as coagulant aid	701:768	The bacteria produced bioflocculant optimally under the following conditions: using sodium carbonate (95.2% flocculating activity) and potassium nitrate (76.6% flocculating activity) as carbon and nitrogen sources, respectively; inoculum size of 3% (v/v); initial pH 9.0; and Al3+ as coagulant aid.
25272753	8	53	theme	S	1259:1259	arg1	proportion					1228:1237	mass proportion	1223:1237	mass proportion (% w/w) of C, N, O, S and P	1223:1265	Fourier transform infrared spectroscopy analysis revealed the presence of hydroxyl, carboxyl and methylene groups in the bioflocculant and energy-dispersive X-ray analysis detected the elemental composition in mass proportion (% w/w) of C, N, O, S and P as 4.12 : 7.40: 39.92: 3.00: 13.91.
25272753	8	53	theme	S	1259:1259	arg1	w/w					1242:1244	% w/w	1240:1244	% w/w	1240:1244	Fourier transform infrared spectroscopy analysis revealed the presence of hydroxyl, carboxyl and methylene groups in the bioflocculant and energy-dispersive X-ray analysis detected the elemental composition in mass proportion (% w/w) of C, N, O, S and P as 4.12 : 7.40: 39.92: 3.00: 13.91.
25272753	2	54	theme	bioflocculant	142:154	arg1	production					156:165	the bioflocculant production	138:165	the bioflocculant production by a Bacillus species isolated from sediment samples of Algoa Bay in the Eastern Cape Province of South Africa	138:276	In our previous study we reported on the bioflocculant production by a Bacillus species isolated from sediment samples of Algoa Bay in the Eastern Cape Province of South Africa.
25272753	8	55	from	composition	1208:1218	arg1	proportion					1228:1237	mass proportion	1223:1237	mass proportion (% w/w) of C, N, O, S and P	1223:1265	Fourier transform infrared spectroscopy analysis revealed the presence of hydroxyl, carboxyl and methylene groups in the bioflocculant and energy-dispersive X-ray analysis detected the elemental composition in mass proportion (% w/w) of C, N, O, S and P as 4.12 : 7.40: 39.92: 3.00: 13.91.
25272753	8	55	from	composition	1208:1218	arg1	w/w					1242:1244	% w/w	1240:1244	% w/w	1240:1244	Fourier transform infrared spectroscopy analysis revealed the presence of hydroxyl, carboxyl and methylene groups in the bioflocculant and energy-dispersive X-ray analysis detected the elemental composition in mass proportion (% w/w) of C, N, O, S and P as 4.12 : 7.40: 39.92: 3.00: 13.91.
25272753	9	56	theme	amorphous	1371:1379	arg1	compound					1381:1388	an amorphous compound	1368:1388	an amorphous compound	1368:1388	Scanning electron micrograph image of the bioflocculant revealed an amorphous compound.
25272753	4	57	theme	following	528:536	arg1	conditions					538:547	the following conditions	524:547	the following conditions	524:547	The bacteria produced bioflocculant optimally under the following conditions: using sodium carbonate (95.2% flocculating activity) and potassium nitrate (76.6% flocculating activity) as carbon and nitrogen sources, respectively; inoculum size of 3% (v/v); initial pH 9.0; and Al3+ as coagulant aid.
25272753	3	58	theme	current	282:288	arg1	study					290:294	current study	282:294	current study	282:294	In current study we carried out further evaluation on the effect of different culture conditions on the bioflocculant production, as well as characterised the bioflocculant produced in detail.
25272753	2	59	theme	sediment	203:210	arg1	samples					212:218	sediment samples	203:218	sediment samples of Algoa Bay	203:231	In our previous study we reported on the bioflocculant production by a Bacillus species isolated from sediment samples of Algoa Bay in the Eastern Cape Province of South Africa.
25272753	4	60	theme	pH	736:737	arg1	size					710:713	inoculum size	701:713	inoculum size of 3% (v/v); initial pH 9.0; and Al3+ as coagulant aid	701:768	The bacteria produced bioflocculant optimally under the following conditions: using sodium carbonate (95.2% flocculating activity) and potassium nitrate (76.6% flocculating activity) as carbon and nitrogen sources, respectively; inoculum size of 3% (v/v); initial pH 9.0; and Al3+ as coagulant aid.
25272753	4	61	theme	initial	728:734	arg1	pH					736:737	initial pH 9.0	728:741	initial pH 9.0	728:741	The bacteria produced bioflocculant optimally under the following conditions: using sodium carbonate (95.2% flocculating activity) and potassium nitrate (76.6% flocculating activity) as carbon and nitrogen sources, respectively; inoculum size of 3% (v/v); initial pH 9.0; and Al3+ as coagulant aid.
25272753	2	62	theme	Eastern	240:246	arg1	Province					253:260	the Eastern Cape Province	236:260	the Eastern Cape Province of South Africa	236:276	In our previous study we reported on the bioflocculant production by a Bacillus species isolated from sediment samples of Algoa Bay in the Eastern Cape Province of South Africa.
25272753	7	63	theme	Gilbert	922:928	arg1	bioflocculant					939:951	Gilbert purified bioflocculant	922:951	Gilbert purified bioflocculant	922:951	Gilbert purified bioflocculant demonstrated that it was composed mainly of polysaccharide.
25272753	8	64	theme	C	1250:1250	arg1	proportion					1228:1237	mass proportion	1223:1237	mass proportion (% w/w) of C, N, O, S and P	1223:1265	Fourier transform infrared spectroscopy analysis revealed the presence of hydroxyl, carboxyl and methylene groups in the bioflocculant and energy-dispersive X-ray analysis detected the elemental composition in mass proportion (% w/w) of C, N, O, S and P as 4.12 : 7.40: 39.92: 3.00: 13.91.
25272753	8	64	theme	C	1250:1250	arg1	w/w					1242:1244	% w/w	1240:1244	% w/w	1240:1244	Fourier transform infrared spectroscopy analysis revealed the presence of hydroxyl, carboxyl and methylene groups in the bioflocculant and energy-dispersive X-ray analysis detected the elemental composition in mass proportion (% w/w) of C, N, O, S and P as 4.12 : 7.40: 39.92: 3.00: 13.91.
25272753	8	65	theme	N	1253:1253	arg1	proportion					1228:1237	mass proportion	1223:1237	mass proportion (% w/w) of C, N, O, S and P	1223:1265	Fourier transform infrared spectroscopy analysis revealed the presence of hydroxyl, carboxyl and methylene groups in the bioflocculant and energy-dispersive X-ray analysis detected the elemental composition in mass proportion (% w/w) of C, N, O, S and P as 4.12 : 7.40: 39.92: 3.00: 13.91.
25272753	8	65	theme	N	1253:1253	arg1	w/w					1242:1244	% w/w	1240:1244	% w/w	1240:1244	Fourier transform infrared spectroscopy analysis revealed the presence of hydroxyl, carboxyl and methylene groups in the bioflocculant and energy-dispersive X-ray analysis detected the elemental composition in mass proportion (% w/w) of C, N, O, S and P as 4.12 : 7.40: 39.92: 3.00: 13.91.
25272753	8	66	theme	O	1256:1256	arg1	proportion					1228:1237	mass proportion	1223:1237	mass proportion (% w/w) of C, N, O, S and P	1223:1265	Fourier transform infrared spectroscopy analysis revealed the presence of hydroxyl, carboxyl and methylene groups in the bioflocculant and energy-dispersive X-ray analysis detected the elemental composition in mass proportion (% w/w) of C, N, O, S and P as 4.12 : 7.40: 39.92: 3.00: 13.91.
25272753	8	66	theme	O	1256:1256	arg1	w/w					1242:1244	% w/w	1240:1244	% w/w	1240:1244	Fourier transform infrared spectroscopy analysis revealed the presence of hydroxyl, carboxyl and methylene groups in the bioflocculant and energy-dispersive X-ray analysis detected the elemental composition in mass proportion (% w/w) of C, N, O, S and P as 4.12 : 7.40: 39.92: 3.00: 13.91.
25272753	4	67	theme	flocculating	580:591	arg1	activity					593:600	95.2% flocculating activity	574:600	95.2% flocculating activity	574:600	The bacteria produced bioflocculant optimally under the following conditions: using sodium carbonate (95.2% flocculating activity) and potassium nitrate (76.6% flocculating activity) as carbon and nitrogen sources, respectively; inoculum size of 3% (v/v); initial pH 9.0; and Al3+ as coagulant aid.
25272753	4	67	theme	flocculating	580:591	arg1	carbonate					563:571	sodium carbonate	556:571	sodium carbonate (95.2% flocculating activity)	556:601	The bacteria produced bioflocculant optimally under the following conditions: using sodium carbonate (95.2% flocculating activity) and potassium nitrate (76.6% flocculating activity) as carbon and nitrogen sources, respectively; inoculum size of 3% (v/v); initial pH 9.0; and Al3+ as coagulant aid.
25272753	2	68	theme	Bacillus	172:179	arg1	species					181:187	a Bacillus species	170:187	a Bacillus species isolated from sediment samples of Algoa Bay in the Eastern Cape Province of South Africa	170:276	In our previous study we reported on the bioflocculant production by a Bacillus species isolated from sediment samples of Algoa Bay in the Eastern Cape Province of South Africa.
25272753	4	69	theme	carbon	658:663	arg1	carbonate					563:571	sodium carbonate	556:571	sodium carbonate (95.2% flocculating activity)	556:601	The bacteria produced bioflocculant optimally under the following conditions: using sodium carbonate (95.2% flocculating activity) and potassium nitrate (76.6% flocculating activity) as carbon and nitrogen sources, respectively; inoculum size of 3% (v/v); initial pH 9.0; and Al3+ as coagulant aid.
25272753	4	69	theme	carbon	658:663	arg1	nitrate					617:623	potassium nitrate	607:623	potassium nitrate (76.6% flocculating activity)	607:653	The bacteria produced bioflocculant optimally under the following conditions: using sodium carbonate (95.2% flocculating activity) and potassium nitrate (76.6% flocculating activity) as carbon and nitrogen sources, respectively; inoculum size of 3% (v/v); initial pH 9.0; and Al3+ as coagulant aid.
25272753	4	69	theme	carbon	658:663	arg1	sources					678:684	carbon and nitrogen sources	658:684	sources	678:684	The bacteria produced bioflocculant optimally under the following conditions: using sodium carbonate (95.2% flocculating activity) and potassium nitrate (76.6% flocculating activity) as carbon and nitrogen sources, respectively; inoculum size of 3% (v/v); initial pH 9.0; and Al3+ as coagulant aid.
25272753	9	70	theme	micrograph	1321:1330	arg1	image					1332:1336	Scanning electron micrograph image	1303:1336	Scanning electron micrograph image of the bioflocculant	1303:1357	Scanning electron micrograph image of the bioflocculant revealed an amorphous compound.
25272753	2	71	theme	Bay	229:231	arg1	samples					212:218	sediment samples	203:218	sediment samples of Algoa Bay	203:231	In our previous study we reported on the bioflocculant production by a Bacillus species isolated from sediment samples of Algoa Bay in the Eastern Cape Province of South Africa.
25272753	5	72	theme	residual	810:817	arg1	activity					832:839	44.2% residual flocculating activity	804:839	44.2% residual flocculating activity	804:839	The crude bioflocculant retained 44.2% residual flocculating activity after heating at 100 degrees C for 15 min.
27474547	0	0	theme	composites	101:110	arg1	polymerization					57:70	in situ polymerization	49:70	in situ polymerization of polypyrrole-nanocellulose composites	49:110	Advancement in conductive cotton fabrics through in situ polymerization of polypyrrole-nanocellulose composites.
27474547	3	1	theme	fabrics/FeCl3/H2O	542:558	arg1	system					560:565	the pyrrole/cotton fabrics/FeCl3/H2O system	523:565	the pyrrole/cotton fabrics/FeCl3/H2O system	523:565	It is anticipated that incorporation of nanocellulose in the pyrrole/cotton fabrics/FeCl3/H2O system would form an integral part of the composites with mechanical, electrical or both properties.
27474547	7	2	theme	conductive	933:942	arg1	properties					968:977	The conductive, mechanical and thermal properties	929:977	properties	968:977	The conductive, mechanical and thermal properties of cotton fabrics coated independently with different nanocellulose/polypyrrole were investigated.
27474547	0	3	theme	polypyrrole-nanocellulose	75:99	arg1	composites					101:110	polypyrrole-nanocellulose composites	75:110	polypyrrole-nanocellulose composites	75:110	Advancement in conductive cotton fabrics through in situ polymerization of polypyrrole-nanocellulose composites.
27474547	1	4	theme	new	172:174	arg1	approach					176:183	a new approach	170:183	a new approach for development of conductive - coated textile materials	170:240	Current research was undertaking with a view to innovate a new approach for development of conductive - coated textile materials through coating cotton fabrics with nanocellulose/polypyrrole composites.
27474547	9	5	theme	nanocellulose	1296:1308	arg1	substrate					1310:1318	nanocellulose substrate	1296:1318	nanocellulose substrate used	1296:1323	Results signify that, the conductivity of cotton fabrics increases exponentially with increasing the dose of pyrrole and oxidant irrespective of nanocellulose substrate used.
27474547	0	6	from	Advancement	0:10	arg1	fabrics					33:39	conductive cotton fabrics	15:39	conductive cotton fabrics	15:39	Advancement in conductive cotton fabrics through in situ polymerization of polypyrrole-nanocellulose composites.
27474547	7	7	theme	different	1023:1031	arg1	nanocellulose/polypyrrole					1033:1057	different nanocellulose/polypyrrole	1023:1057	different nanocellulose/polypyrrole	1023:1057	The conductive, mechanical and thermal properties of cotton fabrics coated independently with different nanocellulose/polypyrrole were investigated.
27474547	4	8	theme	fabrics	800:806	arg1	presence					781:788	presence	781:788	presence of cotton fabrics	781:806	Three different nanocellulosic substrates are involved in the oxidation polymerization reaction of polypyrrole (Ppy) in presence of cotton fabrics.
27474547	1	9	theme	nanocellulose/polypyrrole	278:302	arg1	composites					304:313	nanocellulose/polypyrrole composites	278:313	nanocellulose/polypyrrole composites	278:313	Current research was undertaking with a view to innovate a new approach for development of conductive - coated textile materials through coating cotton fabrics with nanocellulose/polypyrrole composites.
27474547	8	10	used	used	1116:1119	arg2	XRD					1089:1091	XRD	1089:1091	XRD	1089:1091	FTIR, TGA, XRD, SEM and EDX were also used for further characterization.
27474547	8	10	used	used	1116:1119	arg2	EDX					1102:1104	EDX	1102:1104	EDX	1102:1104	FTIR, TGA, XRD, SEM and EDX were also used for further characterization.
27474547	8	10	used	used	1116:1119	arg2	TGA					1084:1086	TGA	1084:1086	TGA	1084:1086	FTIR, TGA, XRD, SEM and EDX were also used for further characterization.
27474547	8	10	used	used	1116:1119	arg2	FTIR					1078:1081	FTIR	1078:1081	FTIR	1078:1081	FTIR, TGA, XRD, SEM and EDX were also used for further characterization.
27474547	8	10	used	used	1116:1119	arg2	SEM					1094:1096	SEM	1094:1096	SEM	1094:1096	FTIR, TGA, XRD, SEM and EDX were also used for further characterization.
27474547	10	11	theme	cotton	1362:1367	arg1	fabrics					1369:1375	cotton fabrics	1362:1375	cotton fabrics	1362:1375	While, the mechanical properties of cotton fabrics are not significantly affected by the oxidant treatment.
27474547	3	12	theme	mechanical	618:627	arg1	properties					649:658	mechanical, electrical or both properties	618:658	mechanical, electrical or both properties	618:658	It is anticipated that incorporation of nanocellulose in the pyrrole/cotton fabrics/FeCl3/H2O system would form an integral part of the composites with mechanical, electrical or both properties.
27474547	4	13	theme	polypyrrole	760:770	arg1	reaction					748:755	the oxidation polymerization reaction	719:755	the oxidation polymerization reaction of polypyrrole (Ppy) in presence of cotton fabrics	719:806	Three different nanocellulosic substrates are involved in the oxidation polymerization reaction of polypyrrole (Ppy) in presence of cotton fabrics.
27474547	5	14	theme	pyrrole	896:902	arg1	viz					904:906	pyrrole viz	896:906	pyrrole viz	896:906	Polymerization was subsequently carried out by admixing at various ratios of FeCl3 and pyrrole viz.
27474547	7	15	theme	cotton	982:987	arg1	fabrics					989:995	cotton fabrics	982:995	cotton fabrics coated independently with different nanocellulose/polypyrrole	982:1057	The conductive, mechanical and thermal properties of cotton fabrics coated independently with different nanocellulose/polypyrrole were investigated.
27474547	3	16	theme	integral	581:588	arg1	part					590:593	an integral part	578:593	an integral part of the composites	578:611	It is anticipated that incorporation of nanocellulose in the pyrrole/cotton fabrics/FeCl3/H2O system would form an integral part of the composites with mechanical, electrical or both properties.
27474547	4	17	from	reaction	748:755	arg1	presence					781:788	presence	781:788	presence of cotton fabrics	781:806	Three different nanocellulosic substrates are involved in the oxidation polymerization reaction of polypyrrole (Ppy) in presence of cotton fabrics.
27474547	5	18	theme	various	868:874	arg1	ratios					876:881	various ratios	868:881	various ratios of FeCl3 and pyrrole viz	868:906	Polymerization was subsequently carried out by admixing at various ratios of FeCl3 and pyrrole viz.
27474547	7	19	theme	fabrics	989:995	arg1	properties					968:977	The conductive, mechanical and thermal properties	929:977	properties	968:977	The conductive, mechanical and thermal properties of cotton fabrics coated independently with different nanocellulose/polypyrrole were investigated.
27474547	4	20	theme	cotton	793:798	arg1	fabrics					800:806	cotton fabrics	793:806	cotton fabrics	793:806	Three different nanocellulosic substrates are involved in the oxidation polymerization reaction of polypyrrole (Ppy) in presence of cotton fabrics.
27474547	10	21	theme	mechanical	1337:1346	arg1	properties					1348:1357	the mechanical properties	1333:1357	the mechanical properties of cotton fabrics	1333:1375	While, the mechanical properties of cotton fabrics are not significantly affected by the oxidant treatment.
27474547	4	22	theme	polymerization	733:746	arg1	reaction					748:755	the oxidation polymerization reaction	719:755	the oxidation polymerization reaction of polypyrrole (Ppy) in presence of cotton fabrics	719:806	Three different nanocellulosic substrates are involved in the oxidation polymerization reaction of polypyrrole (Ppy) in presence of cotton fabrics.
27474547	9	23	theme	oxidant	1272:1278	arg1	dose					1252:1255	the dose	1248:1255	the dose of pyrrole and oxidant irrespective of nanocellulose substrate used	1248:1323	Results signify that, the conductivity of cotton fabrics increases exponentially with increasing the dose of pyrrole and oxidant irrespective of nanocellulose substrate used.
27474547	1	24	theme	Current	113:119	arg1	research					121:128	Current research	113:128	Current research	113:128	Current research was undertaking with a view to innovate a new approach for development of conductive - coated textile materials through coating cotton fabrics with nanocellulose/polypyrrole composites.
27474547	2	25	theme	nanocellulose	393:405	arg1	role					385:388	the role	381:388	the role of nanocellulose	381:405	The study was designed in order to have a clear understanding of the role of nanocellulose as well as modified composite thereof under investigation.
27474547	0	26	theme	cotton	26:31	arg1	fabrics					33:39	conductive cotton fabrics	15:39	conductive cotton fabrics	15:39	Advancement in conductive cotton fabrics through in situ polymerization of polypyrrole-nanocellulose composites.
27474547	8	27	theme	further	1125:1131	arg1	characterization					1133:1148	further characterization	1125:1148	further characterization	1125:1148	FTIR, TGA, XRD, SEM and EDX were also used for further characterization.
27474547	9	28	theme	irrespective	1280:1291	arg1	pyrrole					1260:1266	pyrrole	1260:1266	pyrrole	1260:1266	Results signify that, the conductivity of cotton fabrics increases exponentially with increasing the dose of pyrrole and oxidant irrespective of nanocellulose substrate used.
27474547	1	29	theme	conductive	204:213	arg1	materials					232:240	conductive - coated textile materials	204:240	conductive - coated textile materials	204:240	Current research was undertaking with a view to innovate a new approach for development of conductive - coated textile materials through coating cotton fabrics with nanocellulose/polypyrrole composites.
27474547	0	30	theme	conductive	15:24	arg1	fabrics					33:39	conductive cotton fabrics	15:39	conductive cotton fabrics	15:39	Advancement in conductive cotton fabrics through in situ polymerization of polypyrrole-nanocellulose composites.
27474547	3	31	from	incorporation	489:501	arg1	system					560:565	the pyrrole/cotton fabrics/FeCl3/H2O system	523:565	the pyrrole/cotton fabrics/FeCl3/H2O system	523:565	It is anticipated that incorporation of nanocellulose in the pyrrole/cotton fabrics/FeCl3/H2O system would form an integral part of the composites with mechanical, electrical or both properties.
27474547	3	32	theme	composites	602:611	arg1	part					590:593	an integral part	578:593	an integral part of the composites	578:611	It is anticipated that incorporation of nanocellulose in the pyrrole/cotton fabrics/FeCl3/H2O system would form an integral part of the composites with mechanical, electrical or both properties.
27474547	4	33	theme	nanocellulosic	677:690	arg1	substrates					692:701	Three different nanocellulosic substrates	661:701	Three different nanocellulosic substrates	661:701	Three different nanocellulosic substrates are involved in the oxidation polymerization reaction of polypyrrole (Ppy) in presence of cotton fabrics.
27474547	3	34	theme	pyrrole/cotton	527:540	arg1	system					560:565	the pyrrole/cotton fabrics/FeCl3/H2O system	523:565	the pyrrole/cotton fabrics/FeCl3/H2O system	523:565	It is anticipated that incorporation of nanocellulose in the pyrrole/cotton fabrics/FeCl3/H2O system would form an integral part of the composites with mechanical, electrical or both properties.
27474547	5	35	theme	viz	904:906	arg1	ratios					876:881	various ratios	868:881	various ratios of FeCl3 and pyrrole viz	868:906	Polymerization was subsequently carried out by admixing at various ratios of FeCl3 and pyrrole viz.
27474547	4	36	theme	oxidation	723:731	arg1	reaction					748:755	the oxidation polymerization reaction	719:755	the oxidation polymerization reaction of polypyrrole (Ppy) in presence of cotton fabrics	719:806	Three different nanocellulosic substrates are involved in the oxidation polymerization reaction of polypyrrole (Ppy) in presence of cotton fabrics.
27474547	1	37	theme	coated	217:222	arg1	materials					232:240	conductive - coated textile materials	204:240	conductive - coated textile materials	204:240	Current research was undertaking with a view to innovate a new approach for development of conductive - coated textile materials through coating cotton fabrics with nanocellulose/polypyrrole composites.
27474547	9	38	theme	substrate	1310:1318	arg1	irrespective					1280:1291	irrespective	1280:1291	irrespective	1280:1291	Results signify that, the conductivity of cotton fabrics increases exponentially with increasing the dose of pyrrole and oxidant irrespective of nanocellulose substrate used.
27474547	0	39	dep	in	49:50	arg1	situ					52:55	situ	52:55	situ	52:55	Advancement in conductive cotton fabrics through in situ polymerization of polypyrrole-nanocellulose composites.
27474547	10	40	theme	oxidant	1415:1421	arg1	treatment					1423:1431	the oxidant treatment	1411:1431	the oxidant treatment	1411:1431	While, the mechanical properties of cotton fabrics are not significantly affected by the oxidant treatment.
27474547	2	41	theme	modified	418:425	arg1	composite					427:435	modified composite	418:435	a clear understanding of the role of nanocellulose as well as modified composite thereof under investigation	356:463	The study was designed in order to have a clear understanding of the role of nanocellulose as well as modified composite thereof under investigation.
27474547	2	41	theme	modified	418:425	arg1	role					385:388	the role	381:388	the role of nanocellulose	381:405	The study was designed in order to have a clear understanding of the role of nanocellulose as well as modified composite thereof under investigation.
27474547	10	42	theme	fabrics	1369:1375	arg1	properties					1348:1357	the mechanical properties	1333:1357	the mechanical properties of cotton fabrics	1333:1375	While, the mechanical properties of cotton fabrics are not significantly affected by the oxidant treatment.
27474547	0	43	theme	in	49:50	arg1	polymerization					57:70	in situ polymerization	49:70	in situ polymerization of polypyrrole-nanocellulose composites	49:110	Advancement in conductive cotton fabrics through in situ polymerization of polypyrrole-nanocellulose composites.
27474547	3	44	theme	electrical	630:639	arg1	properties					649:658	mechanical, electrical or both properties	618:658	mechanical, electrical or both properties	618:658	It is anticipated that incorporation of nanocellulose in the pyrrole/cotton fabrics/FeCl3/H2O system would form an integral part of the composites with mechanical, electrical or both properties.
27474547	1	45	with	fabrics	265:271	arg1	composites					304:313	nanocellulose/polypyrrole composites	278:313	nanocellulose/polypyrrole composites	278:313	Current research was undertaking with a view to innovate a new approach for development of conductive - coated textile materials through coating cotton fabrics with nanocellulose/polypyrrole composites.
27474547	2	46	theme	clear	358:362	arg1	understanding					364:376	a clear understanding	356:376	a clear understanding of the role of nanocellulose as well as modified composite thereof under investigation	356:463	The study was designed in order to have a clear understanding of the role of nanocellulose as well as modified composite thereof under investigation.
27474547	9	47	theme	pyrrole	1260:1266	arg1	dose					1252:1255	the dose	1248:1255	the dose of pyrrole and oxidant irrespective of nanocellulose substrate used	1248:1323	Results signify that, the conductivity of cotton fabrics increases exponentially with increasing the dose of pyrrole and oxidant irrespective of nanocellulose substrate used.
27474547	3	48	theme	both	644:647	arg1	properties					649:658	mechanical, electrical or both properties	618:658	mechanical, electrical or both properties	618:658	It is anticipated that incorporation of nanocellulose in the pyrrole/cotton fabrics/FeCl3/H2O system would form an integral part of the composites with mechanical, electrical or both properties.
27474547	3	49	theme	nanocellulose	506:518	arg1	incorporation					489:501	incorporation	489:501	incorporation of nanocellulose in the pyrrole/cotton fabrics/FeCl3/H2O system	489:565	It is anticipated that incorporation of nanocellulose in the pyrrole/cotton fabrics/FeCl3/H2O system would form an integral part of the composites with mechanical, electrical or both properties.
27474547	7	50	theme	thermal	960:966	arg1	properties					968:977	The conductive, mechanical and thermal properties	929:977	properties	968:977	The conductive, mechanical and thermal properties of cotton fabrics coated independently with different nanocellulose/polypyrrole were investigated.
27474547	1	51	theme	materials	232:240	arg1	development					189:199	development	189:199	development of conductive - coated textile materials	189:240	Current research was undertaking with a view to innovate a new approach for development of conductive - coated textile materials through coating cotton fabrics with nanocellulose/polypyrrole composites.
27474547	9	52	theme	cotton	1193:1198	arg1	fabrics					1200:1206	cotton fabrics	1193:1206	cotton fabrics	1193:1206	Results signify that, the conductivity of cotton fabrics increases exponentially with increasing the dose of pyrrole and oxidant irrespective of nanocellulose substrate used.
27474547	9	53	theme	fabrics	1200:1206	arg1	conductivity					1177:1188	the conductivity	1173:1188	the conductivity of cotton fabrics	1173:1206	Results signify that, the conductivity of cotton fabrics increases exponentially with increasing the dose of pyrrole and oxidant irrespective of nanocellulose substrate used.
27474547	1	54	theme	textile	224:230	arg1	materials					232:240	conductive - coated textile materials	204:240	conductive - coated textile materials	204:240	Current research was undertaking with a view to innovate a new approach for development of conductive - coated textile materials through coating cotton fabrics with nanocellulose/polypyrrole composites.
27474547	4	55	theme	different	667:675	arg1	substrates					692:701	Three different nanocellulosic substrates	661:701	Three different nanocellulosic substrates	661:701	Three different nanocellulosic substrates are involved in the oxidation polymerization reaction of polypyrrole (Ppy) in presence of cotton fabrics.
27474547	7	56	theme	mechanical	945:954	arg1	properties					968:977	The conductive, mechanical and thermal properties	929:977	properties	968:977	The conductive, mechanical and thermal properties of cotton fabrics coated independently with different nanocellulose/polypyrrole were investigated.
27474547	1	57	theme	coating	250:256	arg1	fabrics					265:271	coating cotton fabrics	250:271	coating cotton fabrics with nanocellulose/polypyrrole composites	250:313	Current research was undertaking with a view to innovate a new approach for development of conductive - coated textile materials through coating cotton fabrics with nanocellulose/polypyrrole composites.
27474547	5	58	theme	FeCl3	886:890	arg1	ratios					876:881	various ratios	868:881	various ratios of FeCl3 and pyrrole viz	868:906	Polymerization was subsequently carried out by admixing at various ratios of FeCl3 and pyrrole viz.
27474547	2	59	theme	role	385:388	arg1	composite					427:435	modified composite	418:435	a clear understanding of the role of nanocellulose as well as modified composite thereof under investigation	356:463	The study was designed in order to have a clear understanding of the role of nanocellulose as well as modified composite thereof under investigation.
27474547	2	59	theme	role	385:388	arg1	understanding					364:376	a clear understanding	356:376	a clear understanding of the role of nanocellulose as well as modified composite thereof under investigation	356:463	The study was designed in order to have a clear understanding of the role of nanocellulose as well as modified composite thereof under investigation.
27474547	2	59	theme	role	385:388	arg1	role					385:388	the role	381:388	the role of nanocellulose	381:405	The study was designed in order to have a clear understanding of the role of nanocellulose as well as modified composite thereof under investigation.
27474547	1	60	theme	cotton	258:263	arg1	fabrics					265:271	coating cotton fabrics	250:271	coating cotton fabrics with nanocellulose/polypyrrole composites	250:313	Current research was undertaking with a view to innovate a new approach for development of conductive - coated textile materials through coating cotton fabrics with nanocellulose/polypyrrole composites.
24462668	7	0	from	residue	1606:1612	arg1	residue					1531:1537	an α-L-Araf residue	1519:1537	either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp	1512:1841	Analysis of RE oligosaccharides, both 2AB labelled (from W-sol Fr) and glycosyl-alditol (from KOH-sol Fr), revealed that the RE glycosyl sequence of wheat endosperm AX comprises a linear (1→4)-β-D-Xylp backbone which may be mono-substituted with either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp.
24462668	2	1	theme	RE	451:452	arg1	s					464:464	the reducing end (RE) sequence(s)	433:465	the reducing end (RE) sequence(s) of wheat (monocot) AXs	433:488	To understand the differential solubility properties of the AXs, as well as aspects of their biosynthesis, we are sequencing the xylan backbone and examining the reducing end (RE) sequence(s) of wheat (monocot) AXs.
24462668	6	2	theme	oligosaccharides	1131:1146	arg1	analysis					1112:1119	Detailed structural analysis	1092:1119	Detailed structural analysis of the RE oligosaccharides	1092:1146	Detailed structural analysis of the RE oligosaccharides was performed using a combination of methylation analysis, MALDI-TOF-MS, ESI-QTOF-MS, ESI-MS(n) and enzymic analysis.
24462668	2	3	theme	AXs	486:488	arg1	s					464:464	the reducing end (RE) sequence(s)	433:465	the reducing end (RE) sequence(s) of wheat (monocot) AXs	433:488	To understand the differential solubility properties of the AXs, as well as aspects of their biosynthesis, we are sequencing the xylan backbone and examining the reducing end (RE) sequence(s) of wheat (monocot) AXs.
24462668	2	4	theme	end	446:448	arg1	s					464:464	the reducing end (RE) sequence(s)	433:465	the reducing end (RE) sequence(s) of wheat (monocot) AXs	433:488	To understand the differential solubility properties of the AXs, as well as aspects of their biosynthesis, we are sequencing the xylan backbone and examining the reducing end (RE) sequence(s) of wheat (monocot) AXs.
24462668	6	5	theme	ESI-MS	1234:1239	arg1	combination					1170:1180	a combination	1168:1180	a combination of methylation analysis, MALDI-TOF-MS, ESI-QTOF-MS, ESI-MS(n) and enzymic analysis	1168:1263	Detailed structural analysis of the RE oligosaccharides was performed using a combination of methylation analysis, MALDI-TOF-MS, ESI-QTOF-MS, ESI-MS(n) and enzymic analysis.
24462668	7	6	theme	AX	1431:1432	arg1	endosperm					1421:1429	wheat endosperm AX	1415:1432	wheat endosperm AX	1415:1432	Analysis of RE oligosaccharides, both 2AB labelled (from W-sol Fr) and glycosyl-alditol (from KOH-sol Fr), revealed that the RE glycosyl sequence of wheat endosperm AX comprises a linear (1→4)-β-D-Xylp backbone which may be mono-substituted with either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp.
24462668	7	7	theme	α-D-GlcpA	1708:1716	arg1	residue					1718:1724	an α-D-GlcpA residue	1705:1724	an α-D-GlcpA residue at the reducing end β-D-Xylp residue	1705:1761	Analysis of RE oligosaccharides, both 2AB labelled (from W-sol Fr) and glycosyl-alditol (from KOH-sol Fr), revealed that the RE glycosyl sequence of wheat endosperm AX comprises a linear (1→4)-β-D-Xylp backbone which may be mono-substituted with either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp.
24462668	3	8	theme	grass	511:515	arg1	Miscanthus					555:564	Miscanthus	555:564	Miscanthus	555:564	A previous study of grass AXs (switchgrass, rice, Brachypodium, Miscanthus and foxtail millet) concluded that grasses lacked the comparable RE glycosyl sequence (4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp) found in dicots and gymnosperms but the actual RE sequence was not determined.
24462668	3	8	theme	grass	511:515	arg1	AXs					517:519	grass AXs	511:519	grass AXs (switchgrass, rice, Brachypodium, Miscanthus and foxtail millet)	511:584	A previous study of grass AXs (switchgrass, rice, Brachypodium, Miscanthus and foxtail millet) concluded that grasses lacked the comparable RE glycosyl sequence (4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp) found in dicots and gymnosperms but the actual RE sequence was not determined.
24462668	3	8	theme	grass	511:515	arg1	millet					578:583	foxtail millet	570:583	foxtail millet	570:583	A previous study of grass AXs (switchgrass, rice, Brachypodium, Miscanthus and foxtail millet) concluded that grasses lacked the comparable RE glycosyl sequence (4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp) found in dicots and gymnosperms but the actual RE sequence was not determined.
24462668	3	8	theme	grass	511:515	arg1	rice					535:538	rice	535:538	rice	535:538	A previous study of grass AXs (switchgrass, rice, Brachypodium, Miscanthus and foxtail millet) concluded that grasses lacked the comparable RE glycosyl sequence (4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp) found in dicots and gymnosperms but the actual RE sequence was not determined.
24462668	3	8	theme	grass	511:515	arg1	switchgrass					522:532	switchgrass	522:532	switchgrass	522:532	A previous study of grass AXs (switchgrass, rice, Brachypodium, Miscanthus and foxtail millet) concluded that grasses lacked the comparable RE glycosyl sequence (4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp) found in dicots and gymnosperms but the actual RE sequence was not determined.
24462668	6	9	theme	ESI-QTOF-MS	1221:1231	arg1	combination					1170:1180	a combination	1168:1180	a combination of methylation analysis, MALDI-TOF-MS, ESI-QTOF-MS, ESI-MS(n) and enzymic analysis	1168:1263	Detailed structural analysis of the RE oligosaccharides was performed using a combination of methylation analysis, MALDI-TOF-MS, ESI-QTOF-MS, ESI-MS(n) and enzymic analysis.
24462668	2	10	theme	sequence	455:462	arg1	s					464:464	the reducing end (RE) sequence(s)	433:465	the reducing end (RE) sequence(s) of wheat (monocot) AXs	433:488	To understand the differential solubility properties of the AXs, as well as aspects of their biosynthesis, we are sequencing the xylan backbone and examining the reducing end (RE) sequence(s) of wheat (monocot) AXs.
24462668	1	11	with	-β-D-glucans	195:206	arg1	amounts					225:231	minor amounts	219:231	minor amounts of cellulose	219:244	Walls from wheat (Triticum aestivum L.) endosperm are composed primarily of hetero-(arabino)xylans (AXs) (70%) and (1→3)(1→4)-β-D-glucans (20%) with minor amounts of cellulose and heteromannans (2% each).
24462668	1	11	with	-β-D-glucans	195:206	arg1	heteromannans					250:262	heteromannans	250:262	heteromannans (2% each)	250:272	Walls from wheat (Triticum aestivum L.) endosperm are composed primarily of hetero-(arabino)xylans (AXs) (70%) and (1→3)(1→4)-β-D-glucans (20%) with minor amounts of cellulose and heteromannans (2% each).
24462668	1	11	with	-β-D-glucans	195:206	arg1	cellulose					236:244	cellulose	236:244	cellulose	236:244	Walls from wheat (Triticum aestivum L.) endosperm are composed primarily of hetero-(arabino)xylans (AXs) (70%) and (1→3)(1→4)-β-D-glucans (20%) with minor amounts of cellulose and heteromannans (2% each).
24462668	4	12	theme	RE	871:872	arg1	s					899:899	the RE oligosaccharide sequence(s)	867:900	the RE oligosaccharide sequence(s) of wheat endosperm cell wall AXs	867:933	Here we report the isolation and structural characterisation of the RE oligosaccharide sequence(s) of wheat endosperm cell wall AXs.
24462668	6	13	theme	MALDI-TOF-MS	1207:1218	arg1	combination					1170:1180	a combination	1168:1180	a combination of methylation analysis, MALDI-TOF-MS, ESI-QTOF-MS, ESI-MS(n) and enzymic analysis	1168:1263	Detailed structural analysis of the RE oligosaccharides was performed using a combination of methylation analysis, MALDI-TOF-MS, ESI-QTOF-MS, ESI-MS(n) and enzymic analysis.
24462668	8	14	theme	wheat	1850:1854	arg1	backbones					1869:1877	wheat endosperm AX backbones	1850:1877	wheat endosperm AX backbones	1850:1877	Thus, wheat endosperm AX backbones lacks the RE sequence found in dicot and gymnosperm xylans; a finding consistent with previous reports from other grass species.
24462668	2	15	theme	xylan	404:408	arg1	backbone					410:417	sequencing the xylan backbone	389:417	sequencing the xylan backbone	389:417	To understand the differential solubility properties of the AXs, as well as aspects of their biosynthesis, we are sequencing the xylan backbone and examining the reducing end (RE) sequence(s) of wheat (monocot) AXs.
24462668	2	15	theme	xylan	404:408	arg1	we					382:383	we	382:383	we	382:383	To understand the differential solubility properties of the AXs, as well as aspects of their biosynthesis, we are sequencing the xylan backbone and examining the reducing end (RE) sequence(s) of wheat (monocot) AXs.
24462668	3	16	theme	actual	764:769	arg1	sequence					774:781	the actual RE sequence	760:781	the actual RE sequence	760:781	A previous study of grass AXs (switchgrass, rice, Brachypodium, Miscanthus and foxtail millet) concluded that grasses lacked the comparable RE glycosyl sequence (4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp) found in dicots and gymnosperms but the actual RE sequence was not determined.
24462668	6	17	theme	analysis	1197:1204	arg1	combination					1170:1180	a combination	1168:1180	a combination of methylation analysis, MALDI-TOF-MS, ESI-QTOF-MS, ESI-MS(n) and enzymic analysis	1168:1263	Detailed structural analysis of the RE oligosaccharides was performed using a combination of methylation analysis, MALDI-TOF-MS, ESI-QTOF-MS, ESI-MS(n) and enzymic analysis.
24462668	8	18	theme	AX	1866:1867	arg1	backbones					1869:1877	wheat endosperm AX backbones	1850:1877	wheat endosperm AX backbones	1850:1877	Thus, wheat endosperm AX backbones lacks the RE sequence found in dicot and gymnosperm xylans; a finding consistent with previous reports from other grass species.
24462668	2	19	theme	sequencing	389:398	arg1	backbone					410:417	sequencing the xylan backbone	389:417	sequencing the xylan backbone	389:417	To understand the differential solubility properties of the AXs, as well as aspects of their biosynthesis, we are sequencing the xylan backbone and examining the reducing end (RE) sequence(s) of wheat (monocot) AXs.
24462668	2	19	theme	sequencing	389:398	arg1	we					382:383	we	382:383	we	382:383	To understand the differential solubility properties of the AXs, as well as aspects of their biosynthesis, we are sequencing the xylan backbone and examining the reducing end (RE) sequence(s) of wheat (monocot) AXs.
24462668	4	20	theme	structural	836:845	arg1	characterisation					847:862	structural characterisation	836:862	structural characterisation	836:862	Here we report the isolation and structural characterisation of the RE oligosaccharide sequence(s) of wheat endosperm cell wall AXs.
24462668	8	21	theme	RE	1889:1890	arg1	sequence					1892:1899	the RE sequence	1885:1899	the RE sequence found in dicot and gymnosperm xylans	1885:1936	Thus, wheat endosperm AX backbones lacks the RE sequence found in dicot and gymnosperm xylans; a finding consistent with previous reports from other grass species.
24462668	7	22	theme	β-D-Xyl	1598:1604	arg1	residue					1606:1612	penultimate RE β-D-Xyl residue	1583:1612	penultimate RE β-D-Xyl residue	1583:1612	Analysis of RE oligosaccharides, both 2AB labelled (from W-sol Fr) and glycosyl-alditol (from KOH-sol Fr), revealed that the RE glycosyl sequence of wheat endosperm AX comprises a linear (1→4)-β-D-Xylp backbone which may be mono-substituted with either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp.
24462668	8	23	from	species	1999:2005	arg1	reports					1974:1980	previous reports	1965:1980	previous reports from other grass species	1965:2005	Thus, wheat endosperm AX backbones lacks the RE sequence found in dicot and gymnosperm xylans; a finding consistent with previous reports from other grass species.
24462668	6	24	theme	analysis	1256:1263	arg1	combination					1170:1180	a combination	1168:1180	a combination of methylation analysis, MALDI-TOF-MS, ESI-QTOF-MS, ESI-MS(n) and enzymic analysis	1168:1263	Detailed structural analysis of the RE oligosaccharides was performed using a combination of methylation analysis, MALDI-TOF-MS, ESI-QTOF-MS, ESI-MS(n) and enzymic analysis.
24462668	3	25	located	found	724:728	arg1	gymnosperms					744:754	gymnosperms	744:754	gymnosperms	744:754	A previous study of grass AXs (switchgrass, rice, Brachypodium, Miscanthus and foxtail millet) concluded that grasses lacked the comparable RE glycosyl sequence (4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp) found in dicots and gymnosperms but the actual RE sequence was not determined.
24462668	3	25	located	found	724:728	arg1	dicots					733:738	dicots	733:738	dicots	733:738	A previous study of grass AXs (switchgrass, rice, Brachypodium, Miscanthus and foxtail millet) concluded that grasses lacked the comparable RE glycosyl sequence (4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp) found in dicots and gymnosperms but the actual RE sequence was not determined.
24462668	3	25	located	found	724:728	arg2	-D-Xylp					715:721	4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp	653:721	4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp	653:721	A previous study of grass AXs (switchgrass, rice, Brachypodium, Miscanthus and foxtail millet) concluded that grasses lacked the comparable RE glycosyl sequence (4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp) found in dicots and gymnosperms but the actual RE sequence was not determined.
24462668	3	25	located	found	724:728	arg2	sequence					643:650	the comparable RE glycosyl sequence	616:650	the comparable RE glycosyl sequence (4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp) found in dicots and gymnosperms	616:754	A previous study of grass AXs (switchgrass, rice, Brachypodium, Miscanthus and foxtail millet) concluded that grasses lacked the comparable RE glycosyl sequence (4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp) found in dicots and gymnosperms but the actual RE sequence was not determined.
24462668	7	26	theme	RE	1278:1279	arg1	oligosaccharides					1281:1296	RE oligosaccharides	1278:1296	RE oligosaccharides	1278:1296	Analysis of RE oligosaccharides, both 2AB labelled (from W-sol Fr) and glycosyl-alditol (from KOH-sol Fr), revealed that the RE glycosyl sequence of wheat endosperm AX comprises a linear (1→4)-β-D-Xylp backbone which may be mono-substituted with either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp.
24462668	7	26	theme	RE	1278:1279	arg1	2AB					1304:1306	both 2AB	1299:1306	both 2AB labelled (from W-sol Fr)	1299:1331	Analysis of RE oligosaccharides, both 2AB labelled (from W-sol Fr) and glycosyl-alditol (from KOH-sol Fr), revealed that the RE glycosyl sequence of wheat endosperm AX comprises a linear (1→4)-β-D-Xylp backbone which may be mono-substituted with either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp.
24462668	7	26	theme	RE	1278:1279	arg1	glycosyl-alditol					1337:1352	glycosyl-alditol	1337:1352	glycosyl-alditol (from KOH-sol Fr)	1337:1370	Analysis of RE oligosaccharides, both 2AB labelled (from W-sol Fr) and glycosyl-alditol (from KOH-sol Fr), revealed that the RE glycosyl sequence of wheat endosperm AX comprises a linear (1→4)-β-D-Xylp backbone which may be mono-substituted with either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp.
24462668	4	27	theme	endosperm	911:919	arg1	AXs					931:933	wheat endosperm cell wall AXs	905:933	wheat endosperm cell wall AXs	905:933	Here we report the isolation and structural characterisation of the RE oligosaccharide sequence(s) of wheat endosperm cell wall AXs.
24462668	4	28	dep	isolation	822:830	arg1	the					818:820	the	818:820	the	818:820	Here we report the isolation and structural characterisation of the RE oligosaccharide sequence(s) of wheat endosperm cell wall AXs.
24462668	3	29	theme	RE	631:632	arg1	sequence					643:650	the comparable RE glycosyl sequence	616:650	the comparable RE glycosyl sequence (4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp) found in dicots and gymnosperms	616:754	A previous study of grass AXs (switchgrass, rice, Brachypodium, Miscanthus and foxtail millet) concluded that grasses lacked the comparable RE glycosyl sequence (4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp) found in dicots and gymnosperms but the actual RE sequence was not determined.
24462668	3	29	theme	RE	631:632	arg1	-D-Xylp					715:721	4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp	653:721	4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp	653:721	A previous study of grass AXs (switchgrass, rice, Brachypodium, Miscanthus and foxtail millet) concluded that grasses lacked the comparable RE glycosyl sequence (4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp) found in dicots and gymnosperms but the actual RE sequence was not determined.
24462668	4	30	theme	sequence	890:897	arg1	s					899:899	the RE oligosaccharide sequence(s)	867:900	the RE oligosaccharide sequence(s) of wheat endosperm cell wall AXs	867:933	Here we report the isolation and structural characterisation of the RE oligosaccharide sequence(s) of wheat endosperm cell wall AXs.
24462668	0	31	theme	wall	50:53	arg1	sequence					17:24	The reducing end sequence	0:24	The reducing end sequence of wheat endosperm cell wall	0:53	The reducing end sequence of wheat endosperm cell wall arabinoxylans.
24462668	0	32	theme	end	13:15	arg1	sequence					17:24	The reducing end sequence	0:24	The reducing end sequence of wheat endosperm cell wall	0:53	The reducing end sequence of wheat endosperm cell wall arabinoxylans.
24462668	3	33	theme	1→4	665:667	arg1	sequence					643:650	the comparable RE glycosyl sequence	616:650	the comparable RE glycosyl sequence (4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp) found in dicots and gymnosperms	616:754	A previous study of grass AXs (switchgrass, rice, Brachypodium, Miscanthus and foxtail millet) concluded that grasses lacked the comparable RE glycosyl sequence (4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp) found in dicots and gymnosperms but the actual RE sequence was not determined.
24462668	3	33	theme	1→4	665:667	arg1	-D-Xylp					715:721	4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp	653:721	4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp	653:721	A previous study of grass AXs (switchgrass, rice, Brachypodium, Miscanthus and foxtail millet) concluded that grasses lacked the comparable RE glycosyl sequence (4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp) found in dicots and gymnosperms but the actual RE sequence was not determined.
24462668	8	34	theme	dicot	1910:1914	arg1	xylans					1931:1936	dicot and gymnosperm xylans	1910:1936	dicot and gymnosperm xylans	1910:1936	Thus, wheat endosperm AX backbones lacks the RE sequence found in dicot and gymnosperm xylans; a finding consistent with previous reports from other grass species.
24462668	4	35	theme	s	899:899	arg1	isolation					822:830	isolation	822:830	isolation	822:830	Here we report the isolation and structural characterisation of the RE oligosaccharide sequence(s) of wheat endosperm cell wall AXs.
24462668	4	35	theme	s	899:899	arg1	characterisation					847:862	structural characterisation	836:862	structural characterisation	836:862	Here we report the isolation and structural characterisation of the RE oligosaccharide sequence(s) of wheat endosperm cell wall AXs.
24462668	7	36	dep	labelled	1308:1315	arg1	Fr					1329:1330	W-sol Fr	1323:1330	W-sol Fr	1323:1330	Analysis of RE oligosaccharides, both 2AB labelled (from W-sol Fr) and glycosyl-alditol (from KOH-sol Fr), revealed that the RE glycosyl sequence of wheat endosperm AX comprises a linear (1→4)-β-D-Xylp backbone which may be mono-substituted with either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp.
24462668	7	36	dep	labelled	1308:1315	arg1	2AB					1304:1306	both 2AB	1299:1306	both 2AB labelled (from W-sol Fr)	1299:1331	Analysis of RE oligosaccharides, both 2AB labelled (from W-sol Fr) and glycosyl-alditol (from KOH-sol Fr), revealed that the RE glycosyl sequence of wheat endosperm AX comprises a linear (1→4)-β-D-Xylp backbone which may be mono-substituted with either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp.
24462668	7	37	theme	RE	1595:1596	arg1	residue					1606:1612	penultimate RE β-D-Xyl residue	1583:1612	penultimate RE β-D-Xyl residue	1583:1612	Analysis of RE oligosaccharides, both 2AB labelled (from W-sol Fr) and glycosyl-alditol (from KOH-sol Fr), revealed that the RE glycosyl sequence of wheat endosperm AX comprises a linear (1→4)-β-D-Xylp backbone which may be mono-substituted with either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp.
24462668	8	38	located	found	1901:1905	arg2	sequence					1892:1899	the RE sequence	1885:1899	the RE sequence found in dicot and gymnosperm xylans	1885:1936	Thus, wheat endosperm AX backbones lacks the RE sequence found in dicot and gymnosperm xylans; a finding consistent with previous reports from other grass species.
24462668	8	38	located	found	1901:1905	arg1	xylans					1931:1936	dicot and gymnosperm xylans	1910:1936	dicot and gymnosperm xylans	1910:1936	Thus, wheat endosperm AX backbones lacks the RE sequence found in dicot and gymnosperm xylans; a finding consistent with previous reports from other grass species.
24462668	7	39	dep	-[α-L-Araf-	1629:1639	arg1	-[α-L-Araf-					1778:1788	β-D-Xylp-(1→4)-[α-L-Araf-(1→3)	1764:1793	β-D-Xylp-(1→4)-[α-L-Araf-(1→3)	1764:1793	Analysis of RE oligosaccharides, both 2AB labelled (from W-sol Fr) and glycosyl-alditol (from KOH-sol Fr), revealed that the RE glycosyl sequence of wheat endosperm AX comprises a linear (1→4)-β-D-Xylp backbone which may be mono-substituted with either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp.
24462668	3	40	theme	4-β-D-Xylp-	653:663	arg1	sequence					643:650	the comparable RE glycosyl sequence	616:650	the comparable RE glycosyl sequence (4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp) found in dicots and gymnosperms	616:754	A previous study of grass AXs (switchgrass, rice, Brachypodium, Miscanthus and foxtail millet) concluded that grasses lacked the comparable RE glycosyl sequence (4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp) found in dicots and gymnosperms but the actual RE sequence was not determined.
24462668	3	40	theme	4-β-D-Xylp-	653:663	arg1	-D-Xylp					715:721	4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp	653:721	4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp	653:721	A previous study of grass AXs (switchgrass, rice, Brachypodium, Miscanthus and foxtail millet) concluded that grasses lacked the comparable RE glycosyl sequence (4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp) found in dicots and gymnosperms but the actual RE sequence was not determined.
24462668	8	41	theme	gymnosperm	1920:1929	arg1	xylans					1931:1936	dicot and gymnosperm xylans	1910:1936	dicot and gymnosperm xylans	1910:1936	Thus, wheat endosperm AX backbones lacks the RE sequence found in dicot and gymnosperm xylans; a finding consistent with previous reports from other grass species.
24462668	2	42	theme	wheat	470:474	arg1	AXs					486:488	wheat (monocot) AXs	470:488	wheat (monocot) AXs	470:488	To understand the differential solubility properties of the AXs, as well as aspects of their biosynthesis, we are sequencing the xylan backbone and examining the reducing end (RE) sequence(s) of wheat (monocot) AXs.
24462668	8	43	theme	other	1987:1991	arg1	species					1999:2005	other grass species	1987:2005	other grass species	1987:2005	Thus, wheat endosperm AX backbones lacks the RE sequence found in dicot and gymnosperm xylans; a finding consistent with previous reports from other grass species.
24462668	1	44	theme	cellulose	236:244	arg1	amounts					225:231	minor amounts	219:231	minor amounts of cellulose	219:244	Walls from wheat (Triticum aestivum L.) endosperm are composed primarily of hetero-(arabino)xylans (AXs) (70%) and (1→3)(1→4)-β-D-glucans (20%) with minor amounts of cellulose and heteromannans (2% each).
24462668	1	44	theme	cellulose	236:244	arg1	heteromannans					250:262	heteromannans	250:262	heteromannans (2% each)	250:272	Walls from wheat (Triticum aestivum L.) endosperm are composed primarily of hetero-(arabino)xylans (AXs) (70%) and (1→3)(1→4)-β-D-glucans (20%) with minor amounts of cellulose and heteromannans (2% each).
24462668	1	44	theme	cellulose	236:244	arg1	cellulose					236:244	cellulose	236:244	cellulose	236:244	Walls from wheat (Triticum aestivum L.) endosperm are composed primarily of hetero-(arabino)xylans (AXs) (70%) and (1→3)(1→4)-β-D-glucans (20%) with minor amounts of cellulose and heteromannans (2% each).
24462668	3	45	theme	1→3	680:682	arg1	sequence					643:650	the comparable RE glycosyl sequence	616:650	the comparable RE glycosyl sequence (4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp) found in dicots and gymnosperms	616:754	A previous study of grass AXs (switchgrass, rice, Brachypodium, Miscanthus and foxtail millet) concluded that grasses lacked the comparable RE glycosyl sequence (4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp) found in dicots and gymnosperms but the actual RE sequence was not determined.
24462668	3	45	theme	1→3	680:682	arg1	-D-Xylp					715:721	4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp	653:721	4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp	653:721	A previous study of grass AXs (switchgrass, rice, Brachypodium, Miscanthus and foxtail millet) concluded that grasses lacked the comparable RE glycosyl sequence (4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp) found in dicots and gymnosperms but the actual RE sequence was not determined.
24462668	2	46	theme	biosynthesis	368:379	arg1	properties					317:326	the differential solubility properties	289:326	the differential solubility properties of the AXs	289:337	To understand the differential solubility properties of the AXs, as well as aspects of their biosynthesis, we are sequencing the xylan backbone and examining the reducing end (RE) sequence(s) of wheat (monocot) AXs.
24462668	2	46	theme	biosynthesis	368:379	arg1	aspects					351:357	aspects	351:357	aspects of their biosynthesis	351:379	To understand the differential solubility properties of the AXs, as well as aspects of their biosynthesis, we are sequencing the xylan backbone and examining the reducing end (RE) sequence(s) of wheat (monocot) AXs.
24462668	7	47	theme	-β-D-Xylp-	1800:1809	arg1	-[α-L-Araf-					1629:1639	β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)]	1615:1682	β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)]	1615:1682	Analysis of RE oligosaccharides, both 2AB labelled (from W-sol Fr) and glycosyl-alditol (from KOH-sol Fr), revealed that the RE glycosyl sequence of wheat endosperm AX comprises a linear (1→4)-β-D-Xylp backbone which may be mono-substituted with either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp.
24462668	7	47	theme	-β-D-Xylp-	1800:1809	arg1	-[α-D-GlcAp-					1815:1826	-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)	1800:1831	-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)	1800:1831	Analysis of RE oligosaccharides, both 2AB labelled (from W-sol Fr) and glycosyl-alditol (from KOH-sol Fr), revealed that the RE glycosyl sequence of wheat endosperm AX comprises a linear (1→4)-β-D-Xylp backbone which may be mono-substituted with either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp.
24462668	7	48	from	residue	1568:1574	arg1	residue					1531:1537	an α-L-Araf residue	1519:1537	either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp	1512:1841	Analysis of RE oligosaccharides, both 2AB labelled (from W-sol Fr) and glycosyl-alditol (from KOH-sol Fr), revealed that the RE glycosyl sequence of wheat endosperm AX comprises a linear (1→4)-β-D-Xylp backbone which may be mono-substituted with either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp.
24462668	0	49	theme	wheat	29:33	arg1	wall					50:53	wheat endosperm cell wall	29:53	wheat endosperm cell wall	29:53	The reducing end sequence of wheat endosperm cell wall arabinoxylans.
24462668	3	50	theme	-β-D-Xylp-	669:678	arg1	sequence					643:650	the comparable RE glycosyl sequence	616:650	the comparable RE glycosyl sequence (4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp) found in dicots and gymnosperms	616:754	A previous study of grass AXs (switchgrass, rice, Brachypodium, Miscanthus and foxtail millet) concluded that grasses lacked the comparable RE glycosyl sequence (4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp) found in dicots and gymnosperms but the actual RE sequence was not determined.
24462668	3	50	theme	-β-D-Xylp-	669:678	arg1	-D-Xylp					715:721	4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp	653:721	4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp	653:721	A previous study of grass AXs (switchgrass, rice, Brachypodium, Miscanthus and foxtail millet) concluded that grasses lacked the comparable RE glycosyl sequence (4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp) found in dicots and gymnosperms but the actual RE sequence was not determined.
24462668	4	51	theme	wall	926:929	arg1	AXs					931:933	wheat endosperm cell wall AXs	905:933	wheat endosperm cell wall AXs	905:933	Here we report the isolation and structural characterisation of the RE oligosaccharide sequence(s) of wheat endosperm cell wall AXs.
24462668	7	52	theme	reducing	1546:1553	arg1	residue					1568:1574	the reducing end β-D-Xylp residue	1542:1574	the reducing end β-D-Xylp residue	1542:1574	Analysis of RE oligosaccharides, both 2AB labelled (from W-sol Fr) and glycosyl-alditol (from KOH-sol Fr), revealed that the RE glycosyl sequence of wheat endosperm AX comprises a linear (1→4)-β-D-Xylp backbone which may be mono-substituted with either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp.
24462668	1	53	theme	wheat	81:85	arg1	endosperm					110:118	wheat (Triticum aestivum L.) endosperm	81:118	wheat (Triticum aestivum L.) endosperm	81:118	Walls from wheat (Triticum aestivum L.) endosperm are composed primarily of hetero-(arabino)xylans (AXs) (70%) and (1→3)(1→4)-β-D-glucans (20%) with minor amounts of cellulose and heteromannans (2% each).
24462668	3	54	theme	1→2	695:697	arg1	sequence					643:650	the comparable RE glycosyl sequence	616:650	the comparable RE glycosyl sequence (4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp) found in dicots and gymnosperms	616:754	A previous study of grass AXs (switchgrass, rice, Brachypodium, Miscanthus and foxtail millet) concluded that grasses lacked the comparable RE glycosyl sequence (4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp) found in dicots and gymnosperms but the actual RE sequence was not determined.
24462668	3	54	theme	1→2	695:697	arg1	-D-Xylp					715:721	4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp	653:721	4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp	653:721	A previous study of grass AXs (switchgrass, rice, Brachypodium, Miscanthus and foxtail millet) concluded that grasses lacked the comparable RE glycosyl sequence (4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp) found in dicots and gymnosperms but the actual RE sequence was not determined.
24462668	2	55	theme	solubility	306:315	arg1	properties					317:326	the differential solubility properties	289:326	the differential solubility properties of the AXs	289:337	To understand the differential solubility properties of the AXs, as well as aspects of their biosynthesis, we are sequencing the xylan backbone and examining the reducing end (RE) sequence(s) of wheat (monocot) AXs.
24462668	2	56	theme	monocot	477:483	arg1	AXs					486:488	wheat (monocot) AXs	470:488	wheat (monocot) AXs	470:488	To understand the differential solubility properties of the AXs, as well as aspects of their biosynthesis, we are sequencing the xylan backbone and examining the reducing end (RE) sequence(s) of wheat (monocot) AXs.
24462668	5	57	theme	hot	1026:1028	arg1	water					1030:1034	hot water	1026:1034	hot water (W-sol Fr)	1026:1045	Walls were isolated as an alcohol-insoluble residue (AIR) and sequentially extracted with hot water (W-sol Fr) and 1M KOH containing 1% NaBH4 (KOH-sol Fr).
24462668	5	57	theme	hot	1026:1028	arg1	Fr					1043:1044	W-sol Fr	1037:1044	W-sol Fr	1037:1044	Walls were isolated as an alcohol-insoluble residue (AIR) and sequentially extracted with hot water (W-sol Fr) and 1M KOH containing 1% NaBH4 (KOH-sol Fr).
24462668	6	58	theme	structural	1101:1110	arg1	analysis					1112:1119	Detailed structural analysis	1092:1119	Detailed structural analysis of the RE oligosaccharides	1092:1146	Detailed structural analysis of the RE oligosaccharides was performed using a combination of methylation analysis, MALDI-TOF-MS, ESI-QTOF-MS, ESI-MS(n) and enzymic analysis.
24462668	3	59	theme	-α-L-Rhap-	684:693	arg1	sequence					643:650	the comparable RE glycosyl sequence	616:650	the comparable RE glycosyl sequence (4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp) found in dicots and gymnosperms	616:754	A previous study of grass AXs (switchgrass, rice, Brachypodium, Miscanthus and foxtail millet) concluded that grasses lacked the comparable RE glycosyl sequence (4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp) found in dicots and gymnosperms but the actual RE sequence was not determined.
24462668	3	59	theme	-α-L-Rhap-	684:693	arg1	-D-Xylp					715:721	4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp	653:721	4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp	653:721	A previous study of grass AXs (switchgrass, rice, Brachypodium, Miscanthus and foxtail millet) concluded that grasses lacked the comparable RE glycosyl sequence (4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp) found in dicots and gymnosperms but the actual RE sequence was not determined.
24462668	7	60	theme	α-L-Araf	1522:1529	arg1	residue					1531:1537	an α-L-Araf residue	1519:1537	either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp	1512:1841	Analysis of RE oligosaccharides, both 2AB labelled (from W-sol Fr) and glycosyl-alditol (from KOH-sol Fr), revealed that the RE glycosyl sequence of wheat endosperm AX comprises a linear (1→4)-β-D-Xylp backbone which may be mono-substituted with either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp.
24462668	3	61	theme	1→4	711:713	arg1	sequence					643:650	the comparable RE glycosyl sequence	616:650	the comparable RE glycosyl sequence (4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp) found in dicots and gymnosperms	616:754	A previous study of grass AXs (switchgrass, rice, Brachypodium, Miscanthus and foxtail millet) concluded that grasses lacked the comparable RE glycosyl sequence (4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp) found in dicots and gymnosperms but the actual RE sequence was not determined.
24462668	3	61	theme	1→4	711:713	arg1	-D-Xylp					715:721	4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp	653:721	4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp	653:721	A previous study of grass AXs (switchgrass, rice, Brachypodium, Miscanthus and foxtail millet) concluded that grasses lacked the comparable RE glycosyl sequence (4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp) found in dicots and gymnosperms but the actual RE sequence was not determined.
24462668	2	62	theme	AXs	335:337	arg1	properties					317:326	the differential solubility properties	289:326	the differential solubility properties of the AXs	289:337	To understand the differential solubility properties of the AXs, as well as aspects of their biosynthesis, we are sequencing the xylan backbone and examining the reducing end (RE) sequence(s) of wheat (monocot) AXs.
24462668	2	62	theme	AXs	335:337	arg1	aspects					351:357	aspects	351:357	aspects of their biosynthesis	351:379	To understand the differential solubility properties of the AXs, as well as aspects of their biosynthesis, we are sequencing the xylan backbone and examining the reducing end (RE) sequence(s) of wheat (monocot) AXs.
24462668	8	63	dep	lacks	1879:1883	arg1	finding					1941:1947	a finding	1939:1947	a finding consistent with previous reports from other grass species	1939:2005	Thus, wheat endosperm AX backbones lacks the RE sequence found in dicot and gymnosperm xylans; a finding consistent with previous reports from other grass species.
24462668	1	64	dep	hetero-	146:152	arg1	arabino					154:160	arabino	154:160	arabino	154:160	Walls from wheat (Triticum aestivum L.) endosperm are composed primarily of hetero-(arabino)xylans (AXs) (70%) and (1→3)(1→4)-β-D-glucans (20%) with minor amounts of cellulose and heteromannans (2% each).
24462668	0	65	theme	endosperm	35:43	arg1	wall					50:53	wheat endosperm cell wall	29:53	wheat endosperm cell wall	29:53	The reducing end sequence of wheat endosperm cell wall arabinoxylans.
24462668	3	66	theme	-α-D-GalpA-	699:709	arg1	sequence					643:650	the comparable RE glycosyl sequence	616:650	the comparable RE glycosyl sequence (4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp) found in dicots and gymnosperms	616:754	A previous study of grass AXs (switchgrass, rice, Brachypodium, Miscanthus and foxtail millet) concluded that grasses lacked the comparable RE glycosyl sequence (4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp) found in dicots and gymnosperms but the actual RE sequence was not determined.
24462668	3	66	theme	-α-D-GalpA-	699:709	arg1	-D-Xylp					715:721	4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp	653:721	4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp	653:721	A previous study of grass AXs (switchgrass, rice, Brachypodium, Miscanthus and foxtail millet) concluded that grasses lacked the comparable RE glycosyl sequence (4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp) found in dicots and gymnosperms but the actual RE sequence was not determined.
24462668	7	67	theme	reducing	1733:1740	arg1	residue					1755:1761	the reducing end β-D-Xylp residue	1729:1761	the reducing end β-D-Xylp residue	1729:1761	Analysis of RE oligosaccharides, both 2AB labelled (from W-sol Fr) and glycosyl-alditol (from KOH-sol Fr), revealed that the RE glycosyl sequence of wheat endosperm AX comprises a linear (1→4)-β-D-Xylp backbone which may be mono-substituted with either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp.
24462668	5	68	theme	KOH-sol	1079:1085	arg1	NaBH4					1072:1076	1% NaBH4	1069:1076	1% NaBH4 (KOH-sol Fr)	1069:1089	Walls were isolated as an alcohol-insoluble residue (AIR) and sequentially extracted with hot water (W-sol Fr) and 1M KOH containing 1% NaBH4 (KOH-sol Fr).
24462668	5	68	theme	KOH-sol	1079:1085	arg1	Fr					1087:1088	KOH-sol Fr	1079:1088	KOH-sol Fr	1079:1088	Walls were isolated as an alcohol-insoluble residue (AIR) and sequentially extracted with hot water (W-sol Fr) and 1M KOH containing 1% NaBH4 (KOH-sol Fr).
24462668	7	69	theme	1→4	1454:1456	arg1	backbone					1468:1475	a linear (1→4)-β-D-Xylp backbone	1444:1475	a linear (1→4)-β-D-Xylp backbone which may be mono-substituted with either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp	1444:1841	Analysis of RE oligosaccharides, both 2AB labelled (from W-sol Fr) and glycosyl-alditol (from KOH-sol Fr), revealed that the RE glycosyl sequence of wheat endosperm AX comprises a linear (1→4)-β-D-Xylp backbone which may be mono-substituted with either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp.
24462668	7	69	theme	1→4	1454:1456	arg1	mono-substituted					1490:1505	mono-substituted	1490:1505	mono-substituted	1490:1505	Analysis of RE oligosaccharides, both 2AB labelled (from W-sol Fr) and glycosyl-alditol (from KOH-sol Fr), revealed that the RE glycosyl sequence of wheat endosperm AX comprises a linear (1→4)-β-D-Xylp backbone which may be mono-substituted with either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp.
24462668	7	70	with	mono-substituted	1490:1505	arg1	-β-D-Xylp					1833:1841	either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp	1512:1841	either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp	1512:1841	Analysis of RE oligosaccharides, both 2AB labelled (from W-sol Fr) and glycosyl-alditol (from KOH-sol Fr), revealed that the RE glycosyl sequence of wheat endosperm AX comprises a linear (1→4)-β-D-Xylp backbone which may be mono-substituted with either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp.
24462668	7	71	theme	-β-D-Xylp-	1651:1660	arg1	-[α-L-Araf-					1666:1676	-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)	1651:1681	-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)	1651:1681	Analysis of RE oligosaccharides, both 2AB labelled (from W-sol Fr) and glycosyl-alditol (from KOH-sol Fr), revealed that the RE glycosyl sequence of wheat endosperm AX comprises a linear (1→4)-β-D-Xylp backbone which may be mono-substituted with either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp.
24462668	7	71	theme	-β-D-Xylp-	1651:1660	arg1	-[α-L-Araf-					1629:1639	β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)]	1615:1682	β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)]	1615:1682	Analysis of RE oligosaccharides, both 2AB labelled (from W-sol Fr) and glycosyl-alditol (from KOH-sol Fr), revealed that the RE glycosyl sequence of wheat endosperm AX comprises a linear (1→4)-β-D-Xylp backbone which may be mono-substituted with either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp.
24462668	7	72	theme	linear	1446:1451	arg1	backbone					1468:1475	a linear (1→4)-β-D-Xylp backbone	1444:1475	a linear (1→4)-β-D-Xylp backbone which may be mono-substituted with either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp	1444:1841	Analysis of RE oligosaccharides, both 2AB labelled (from W-sol Fr) and glycosyl-alditol (from KOH-sol Fr), revealed that the RE glycosyl sequence of wheat endosperm AX comprises a linear (1→4)-β-D-Xylp backbone which may be mono-substituted with either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp.
24462668	7	72	theme	linear	1446:1451	arg1	mono-substituted					1490:1505	mono-substituted	1490:1505	mono-substituted	1490:1505	Analysis of RE oligosaccharides, both 2AB labelled (from W-sol Fr) and glycosyl-alditol (from KOH-sol Fr), revealed that the RE glycosyl sequence of wheat endosperm AX comprises a linear (1→4)-β-D-Xylp backbone which may be mono-substituted with either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp.
24462668	1	73	dep	%	266:266	arg1	each					268:271	each	268:271	each	268:271	Walls from wheat (Triticum aestivum L.) endosperm are composed primarily of hetero-(arabino)xylans (AXs) (70%) and (1→3)(1→4)-β-D-glucans (20%) with minor amounts of cellulose and heteromannans (2% each).
24462668	5	74	theme	1	1069:1069	arg1	%					1070:1070	%	1070:1070	%	1070:1070	Walls were isolated as an alcohol-insoluble residue (AIR) and sequentially extracted with hot water (W-sol Fr) and 1M KOH containing 1% NaBH4 (KOH-sol Fr).
24462668	3	75	theme	previous	493:500	arg1	study					502:506	A previous study	491:506	A previous study of grass AXs (switchgrass, rice, Brachypodium, Miscanthus and foxtail millet)	491:584	A previous study of grass AXs (switchgrass, rice, Brachypodium, Miscanthus and foxtail millet) concluded that grasses lacked the comparable RE glycosyl sequence (4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp) found in dicots and gymnosperms but the actual RE sequence was not determined.
24462668	7	76	theme	β-D-Xylp	1746:1753	arg1	residue					1755:1761	the reducing end β-D-Xylp residue	1729:1761	the reducing end β-D-Xylp residue	1729:1761	Analysis of RE oligosaccharides, both 2AB labelled (from W-sol Fr) and glycosyl-alditol (from KOH-sol Fr), revealed that the RE glycosyl sequence of wheat endosperm AX comprises a linear (1→4)-β-D-Xylp backbone which may be mono-substituted with either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp.
24462668	6	77	theme	RE	1128:1129	arg1	oligosaccharides					1131:1146	the RE oligosaccharides	1124:1146	the RE oligosaccharides	1124:1146	Detailed structural analysis of the RE oligosaccharides was performed using a combination of methylation analysis, MALDI-TOF-MS, ESI-QTOF-MS, ESI-MS(n) and enzymic analysis.
24462668	1	78	theme	1→3	186:188	arg1	-β-D-glucans					195:206	(1→3)(1→4)-β-D-glucans	185:206	(1→3)(1→4)-β-D-glucans (20%)	185:212	Walls from wheat (Triticum aestivum L.) endosperm are composed primarily of hetero-(arabino)xylans (AXs) (70%) and (1→3)(1→4)-β-D-glucans (20%) with minor amounts of cellulose and heteromannans (2% each).
24462668	1	78	theme	1→3	186:188	arg1	%					211:211	20%	209:211	20%	209:211	Walls from wheat (Triticum aestivum L.) endosperm are composed primarily of hetero-(arabino)xylans (AXs) (70%) and (1→3)(1→4)-β-D-glucans (20%) with minor amounts of cellulose and heteromannans (2% each).
24462668	2	79	theme	reducing	437:444	arg1	s					464:464	the reducing end (RE) sequence(s)	433:465	the reducing end (RE) sequence(s) of wheat (monocot) AXs	433:488	To understand the differential solubility properties of the AXs, as well as aspects of their biosynthesis, we are sequencing the xylan backbone and examining the reducing end (RE) sequence(s) of wheat (monocot) AXs.
24462668	7	80	theme	endosperm	1421:1429	arg1	sequence					1403:1410	the RE glycosyl sequence	1387:1410	the RE glycosyl sequence of wheat endosperm AX	1387:1432	Analysis of RE oligosaccharides, both 2AB labelled (from W-sol Fr) and glycosyl-alditol (from KOH-sol Fr), revealed that the RE glycosyl sequence of wheat endosperm AX comprises a linear (1→4)-β-D-Xylp backbone which may be mono-substituted with either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp.
24462668	7	81	from	Fr	1368:1369	arg1	Fr					1329:1330	W-sol Fr	1323:1330	W-sol Fr	1323:1330	Analysis of RE oligosaccharides, both 2AB labelled (from W-sol Fr) and glycosyl-alditol (from KOH-sol Fr), revealed that the RE glycosyl sequence of wheat endosperm AX comprises a linear (1→4)-β-D-Xylp backbone which may be mono-substituted with either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp.
24462668	7	81	from	Fr	1368:1369	arg1	2AB					1304:1306	both 2AB	1299:1306	both 2AB labelled (from W-sol Fr)	1299:1331	Analysis of RE oligosaccharides, both 2AB labelled (from W-sol Fr) and glycosyl-alditol (from KOH-sol Fr), revealed that the RE glycosyl sequence of wheat endosperm AX comprises a linear (1→4)-β-D-Xylp backbone which may be mono-substituted with either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp.
24462668	7	81	from	Fr	1368:1369	arg1	oligosaccharides					1281:1296	RE oligosaccharides	1278:1296	RE oligosaccharides	1278:1296	Analysis of RE oligosaccharides, both 2AB labelled (from W-sol Fr) and glycosyl-alditol (from KOH-sol Fr), revealed that the RE glycosyl sequence of wheat endosperm AX comprises a linear (1→4)-β-D-Xylp backbone which may be mono-substituted with either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp.
24462668	7	81	from	Fr	1368:1369	arg1	glycosyl-alditol					1337:1352	glycosyl-alditol	1337:1352	glycosyl-alditol (from KOH-sol Fr)	1337:1370	Analysis of RE oligosaccharides, both 2AB labelled (from W-sol Fr) and glycosyl-alditol (from KOH-sol Fr), revealed that the RE glycosyl sequence of wheat endosperm AX comprises a linear (1→4)-β-D-Xylp backbone which may be mono-substituted with either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp.
24462668	1	82	theme	hetero-	146:152	arg1	%					178:178	70%	176:178	70%	176:178	Walls from wheat (Triticum aestivum L.) endosperm are composed primarily of hetero-(arabino)xylans (AXs) (70%) and (1→3)(1→4)-β-D-glucans (20%) with minor amounts of cellulose and heteromannans (2% each).
24462668	1	82	theme	hetero-	146:152	arg1	AXs					170:172	AXs	170:172	AXs	170:172	Walls from wheat (Triticum aestivum L.) endosperm are composed primarily of hetero-(arabino)xylans (AXs) (70%) and (1→3)(1→4)-β-D-glucans (20%) with minor amounts of cellulose and heteromannans (2% each).
24462668	1	82	theme	hetero-	146:152	arg1	xylans					162:167	hetero-(arabino)xylans	146:167	hetero-(arabino)xylans (AXs) (70%)	146:179	Walls from wheat (Triticum aestivum L.) endosperm are composed primarily of hetero-(arabino)xylans (AXs) (70%) and (1→3)(1→4)-β-D-glucans (20%) with minor amounts of cellulose and heteromannans (2% each).
24462668	3	83	theme	RE	771:772	arg1	sequence					774:781	the actual RE sequence	760:781	the actual RE sequence	760:781	A previous study of grass AXs (switchgrass, rice, Brachypodium, Miscanthus and foxtail millet) concluded that grasses lacked the comparable RE glycosyl sequence (4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp) found in dicots and gymnosperms but the actual RE sequence was not determined.
24462668	4	84	theme	oligosaccharide	874:888	arg1	s					899:899	the RE oligosaccharide sequence(s)	867:900	the RE oligosaccharide sequence(s) of wheat endosperm cell wall AXs	867:933	Here we report the isolation and structural characterisation of the RE oligosaccharide sequence(s) of wheat endosperm cell wall AXs.
24462668	7	85	theme	residue	1718:1724	arg1	-β-D-Xylp					1833:1841	either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp	1512:1841	either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp	1512:1841	Analysis of RE oligosaccharides, both 2AB labelled (from W-sol Fr) and glycosyl-alditol (from KOH-sol Fr), revealed that the RE glycosyl sequence of wheat endosperm AX comprises a linear (1→4)-β-D-Xylp backbone which may be mono-substituted with either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp.
24462668	3	86	theme	AXs	517:519	arg1	study					502:506	A previous study	491:506	A previous study of grass AXs (switchgrass, rice, Brachypodium, Miscanthus and foxtail millet)	491:584	A previous study of grass AXs (switchgrass, rice, Brachypodium, Miscanthus and foxtail millet) concluded that grasses lacked the comparable RE glycosyl sequence (4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp) found in dicots and gymnosperms but the actual RE sequence was not determined.
24462668	7	87	theme	-[α-L-Araf-	1629:1639	arg1	-β-D-Xylp					1833:1841	either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp	1512:1841	either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp	1512:1841	Analysis of RE oligosaccharides, both 2AB labelled (from W-sol Fr) and glycosyl-alditol (from KOH-sol Fr), revealed that the RE glycosyl sequence of wheat endosperm AX comprises a linear (1→4)-β-D-Xylp backbone which may be mono-substituted with either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp.
24462668	8	88	theme	endosperm	1856:1864	arg1	backbones					1869:1877	wheat endosperm AX backbones	1850:1877	wheat endosperm AX backbones	1850:1877	Thus, wheat endosperm AX backbones lacks the RE sequence found in dicot and gymnosperm xylans; a finding consistent with previous reports from other grass species.
24462668	7	89	theme	glycosyl	1394:1401	arg1	sequence					1403:1410	the RE glycosyl sequence	1387:1410	the RE glycosyl sequence of wheat endosperm AX	1387:1432	Analysis of RE oligosaccharides, both 2AB labelled (from W-sol Fr) and glycosyl-alditol (from KOH-sol Fr), revealed that the RE glycosyl sequence of wheat endosperm AX comprises a linear (1→4)-β-D-Xylp backbone which may be mono-substituted with either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp.
24462668	7	90	from	residue	1755:1761	arg1	residue					1718:1724	an α-D-GlcpA residue	1705:1724	an α-D-GlcpA residue at the reducing end β-D-Xylp residue	1705:1761	Analysis of RE oligosaccharides, both 2AB labelled (from W-sol Fr) and glycosyl-alditol (from KOH-sol Fr), revealed that the RE glycosyl sequence of wheat endosperm AX comprises a linear (1→4)-β-D-Xylp backbone which may be mono-substituted with either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp.
24462668	7	91	theme	-β-D-Xylp	1458:1466	arg1	backbone					1468:1475	a linear (1→4)-β-D-Xylp backbone	1444:1475	a linear (1→4)-β-D-Xylp backbone which may be mono-substituted with either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp	1444:1841	Analysis of RE oligosaccharides, both 2AB labelled (from W-sol Fr) and glycosyl-alditol (from KOH-sol Fr), revealed that the RE glycosyl sequence of wheat endosperm AX comprises a linear (1→4)-β-D-Xylp backbone which may be mono-substituted with either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp.
24462668	7	91	theme	-β-D-Xylp	1458:1466	arg1	mono-substituted					1490:1505	mono-substituted	1490:1505	mono-substituted	1490:1505	Analysis of RE oligosaccharides, both 2AB labelled (from W-sol Fr) and glycosyl-alditol (from KOH-sol Fr), revealed that the RE glycosyl sequence of wheat endosperm AX comprises a linear (1→4)-β-D-Xylp backbone which may be mono-substituted with either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp.
24462668	7	92	theme	-β-D-Xylp	1688:1696	arg1	-β-D-Xylp					1833:1841	either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp	1512:1841	either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp	1512:1841	Analysis of RE oligosaccharides, both 2AB labelled (from W-sol Fr) and glycosyl-alditol (from KOH-sol Fr), revealed that the RE glycosyl sequence of wheat endosperm AX comprises a linear (1→4)-β-D-Xylp backbone which may be mono-substituted with either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp.
24462668	8	93	with	consistent	1949:1958	arg1	reports					1974:1980	previous reports	1965:1980	previous reports from other grass species	1965:2005	Thus, wheat endosperm AX backbones lacks the RE sequence found in dicot and gymnosperm xylans; a finding consistent with previous reports from other grass species.
24462668	7	94	theme	KOH-sol	1360:1366	arg1	Fr					1368:1369	KOH-sol Fr	1360:1369	KOH-sol Fr	1360:1369	Analysis of RE oligosaccharides, both 2AB labelled (from W-sol Fr) and glycosyl-alditol (from KOH-sol Fr), revealed that the RE glycosyl sequence of wheat endosperm AX comprises a linear (1→4)-β-D-Xylp backbone which may be mono-substituted with either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp.
24462668	6	95	theme	methylation	1185:1195	arg1	analysis					1197:1204	methylation analysis	1185:1204	methylation analysis	1185:1204	Detailed structural analysis of the RE oligosaccharides was performed using a combination of methylation analysis, MALDI-TOF-MS, ESI-QTOF-MS, ESI-MS(n) and enzymic analysis.
24462668	7	96	theme	β-D-Xylp-	1615:1623	arg1	-[α-L-Araf-					1666:1676	-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)	1651:1681	-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)	1651:1681	Analysis of RE oligosaccharides, both 2AB labelled (from W-sol Fr) and glycosyl-alditol (from KOH-sol Fr), revealed that the RE glycosyl sequence of wheat endosperm AX comprises a linear (1→4)-β-D-Xylp backbone which may be mono-substituted with either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp.
24462668	7	96	theme	β-D-Xylp-	1615:1623	arg1	-[α-D-GlcAp-					1815:1826	-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)	1800:1831	-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)	1800:1831	Analysis of RE oligosaccharides, both 2AB labelled (from W-sol Fr) and glycosyl-alditol (from KOH-sol Fr), revealed that the RE glycosyl sequence of wheat endosperm AX comprises a linear (1→4)-β-D-Xylp backbone which may be mono-substituted with either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp.
24462668	7	96	theme	β-D-Xylp-	1615:1623	arg1	-[α-L-Araf-					1629:1639	β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)]	1615:1682	β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)]	1615:1682	Analysis of RE oligosaccharides, both 2AB labelled (from W-sol Fr) and glycosyl-alditol (from KOH-sol Fr), revealed that the RE glycosyl sequence of wheat endosperm AX comprises a linear (1→4)-β-D-Xylp backbone which may be mono-substituted with either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp.
24462668	7	97	theme	oligosaccharides	1281:1296	arg1	Analysis					1266:1273	Analysis	1266:1273	Analysis of RE oligosaccharides, both 2AB labelled (from W-sol Fr) and glycosyl-alditol (from KOH-sol Fr),	1266:1371	Analysis of RE oligosaccharides, both 2AB labelled (from W-sol Fr) and glycosyl-alditol (from KOH-sol Fr), revealed that the RE glycosyl sequence of wheat endosperm AX comprises a linear (1→4)-β-D-Xylp backbone which may be mono-substituted with either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp.
24462668	4	98	theme	wheat	905:909	arg1	AXs					931:933	wheat endosperm cell wall AXs	905:933	wheat endosperm cell wall AXs	905:933	Here we report the isolation and structural characterisation of the RE oligosaccharide sequence(s) of wheat endosperm cell wall AXs.
24462668	3	99	theme	foxtail	570:576	arg1	AXs					517:519	grass AXs	511:519	grass AXs (switchgrass, rice, Brachypodium, Miscanthus and foxtail millet)	511:584	A previous study of grass AXs (switchgrass, rice, Brachypodium, Miscanthus and foxtail millet) concluded that grasses lacked the comparable RE glycosyl sequence (4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp) found in dicots and gymnosperms but the actual RE sequence was not determined.
24462668	3	99	theme	foxtail	570:576	arg1	millet					578:583	foxtail millet	570:583	foxtail millet	570:583	A previous study of grass AXs (switchgrass, rice, Brachypodium, Miscanthus and foxtail millet) concluded that grasses lacked the comparable RE glycosyl sequence (4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp) found in dicots and gymnosperms but the actual RE sequence was not determined.
24462668	7	100	theme	wheat	1415:1419	arg1	endosperm					1421:1429	wheat endosperm AX	1415:1432	wheat endosperm AX	1415:1432	Analysis of RE oligosaccharides, both 2AB labelled (from W-sol Fr) and glycosyl-alditol (from KOH-sol Fr), revealed that the RE glycosyl sequence of wheat endosperm AX comprises a linear (1→4)-β-D-Xylp backbone which may be mono-substituted with either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp.
24462668	4	101	theme	cell	921:924	arg1	AXs					931:933	wheat endosperm cell wall AXs	905:933	wheat endosperm cell wall AXs	905:933	Here we report the isolation and structural characterisation of the RE oligosaccharide sequence(s) of wheat endosperm cell wall AXs.
24462668	8	102	theme	consistent	1949:1958	arg1	finding					1941:1947	a finding	1939:1947	a finding consistent with previous reports from other grass species	1939:2005	Thus, wheat endosperm AX backbones lacks the RE sequence found in dicot and gymnosperm xylans; a finding consistent with previous reports from other grass species.
24462668	6	103	theme	enzymic	1248:1254	arg1	analysis					1256:1263	enzymic analysis	1248:1263	enzymic analysis	1248:1263	Detailed structural analysis of the RE oligosaccharides was performed using a combination of methylation analysis, MALDI-TOF-MS, ESI-QTOF-MS, ESI-MS(n) and enzymic analysis.
24462668	7	104	theme	RE	1391:1392	arg1	sequence					1403:1410	the RE glycosyl sequence	1387:1410	the RE glycosyl sequence of wheat endosperm AX	1387:1432	Analysis of RE oligosaccharides, both 2AB labelled (from W-sol Fr) and glycosyl-alditol (from KOH-sol Fr), revealed that the RE glycosyl sequence of wheat endosperm AX comprises a linear (1→4)-β-D-Xylp backbone which may be mono-substituted with either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp.
24462668	8	105	theme	previous	1965:1972	arg1	reports					1974:1980	previous reports	1965:1980	previous reports from other grass species	1965:2005	Thus, wheat endosperm AX backbones lacks the RE sequence found in dicot and gymnosperm xylans; a finding consistent with previous reports from other grass species.
24462668	3	106	theme	glycosyl	634:641	arg1	sequence					643:650	the comparable RE glycosyl sequence	616:650	the comparable RE glycosyl sequence (4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp) found in dicots and gymnosperms	616:754	A previous study of grass AXs (switchgrass, rice, Brachypodium, Miscanthus and foxtail millet) concluded that grasses lacked the comparable RE glycosyl sequence (4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp) found in dicots and gymnosperms but the actual RE sequence was not determined.
24462668	3	106	theme	glycosyl	634:641	arg1	-D-Xylp					715:721	4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp	653:721	4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp	653:721	A previous study of grass AXs (switchgrass, rice, Brachypodium, Miscanthus and foxtail millet) concluded that grasses lacked the comparable RE glycosyl sequence (4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp) found in dicots and gymnosperms but the actual RE sequence was not determined.
24462668	1	107	dep	Triticum	88:95	arg1	L.					106:107	Triticum aestivum L.	88:107	Triticum aestivum L.	88:107	Walls from wheat (Triticum aestivum L.) endosperm are composed primarily of hetero-(arabino)xylans (AXs) (70%) and (1→3)(1→4)-β-D-glucans (20%) with minor amounts of cellulose and heteromannans (2% each).
24462668	5	108	theme	alcohol-insoluble	962:978	arg1	Walls					936:940	Walls	936:940	Walls	936:940	Walls were isolated as an alcohol-insoluble residue (AIR) and sequentially extracted with hot water (W-sol Fr) and 1M KOH containing 1% NaBH4 (KOH-sol Fr).
24462668	5	108	theme	alcohol-insoluble	962:978	arg1	AIR					989:991	AIR	989:991	AIR	989:991	Walls were isolated as an alcohol-insoluble residue (AIR) and sequentially extracted with hot water (W-sol Fr) and 1M KOH containing 1% NaBH4 (KOH-sol Fr).
24462668	5	108	theme	alcohol-insoluble	962:978	arg1	residue					980:986	an alcohol-insoluble residue	959:986	an alcohol-insoluble residue (AIR)	959:992	Walls were isolated as an alcohol-insoluble residue (AIR) and sequentially extracted with hot water (W-sol Fr) and 1M KOH containing 1% NaBH4 (KOH-sol Fr).
24462668	1	109	theme	minor	219:223	arg1	amounts					225:231	minor amounts	219:231	minor amounts of cellulose	219:244	Walls from wheat (Triticum aestivum L.) endosperm are composed primarily of hetero-(arabino)xylans (AXs) (70%) and (1→3)(1→4)-β-D-glucans (20%) with minor amounts of cellulose and heteromannans (2% each).
24462668	1	109	theme	minor	219:223	arg1	cellulose					236:244	cellulose	236:244	cellulose	236:244	Walls from wheat (Triticum aestivum L.) endosperm are composed primarily of hetero-(arabino)xylans (AXs) (70%) and (1→3)(1→4)-β-D-glucans (20%) with minor amounts of cellulose and heteromannans (2% each).
24462668	7	110	theme	penultimate	1583:1593	arg1	residue					1606:1612	penultimate RE β-D-Xyl residue	1583:1612	penultimate RE β-D-Xyl residue	1583:1612	Analysis of RE oligosaccharides, both 2AB labelled (from W-sol Fr) and glycosyl-alditol (from KOH-sol Fr), revealed that the RE glycosyl sequence of wheat endosperm AX comprises a linear (1→4)-β-D-Xylp backbone which may be mono-substituted with either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp.
24462668	8	111	theme	grass	1993:1997	arg1	species					1999:2005	other grass species	1987:2005	other grass species	1987:2005	Thus, wheat endosperm AX backbones lacks the RE sequence found in dicot and gymnosperm xylans; a finding consistent with previous reports from other grass species.
24462668	3	112	theme	comparable	620:629	arg1	sequence					643:650	the comparable RE glycosyl sequence	616:650	the comparable RE glycosyl sequence (4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp) found in dicots and gymnosperms	616:754	A previous study of grass AXs (switchgrass, rice, Brachypodium, Miscanthus and foxtail millet) concluded that grasses lacked the comparable RE glycosyl sequence (4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp) found in dicots and gymnosperms but the actual RE sequence was not determined.
24462668	3	112	theme	comparable	620:629	arg1	-D-Xylp					715:721	4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp	653:721	4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp	653:721	A previous study of grass AXs (switchgrass, rice, Brachypodium, Miscanthus and foxtail millet) concluded that grasses lacked the comparable RE glycosyl sequence (4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp) found in dicots and gymnosperms but the actual RE sequence was not determined.
24462668	5	113	theme	W-sol	1037:1041	arg1	water					1030:1034	hot water	1026:1034	hot water (W-sol Fr)	1026:1045	Walls were isolated as an alcohol-insoluble residue (AIR) and sequentially extracted with hot water (W-sol Fr) and 1M KOH containing 1% NaBH4 (KOH-sol Fr).
24462668	5	113	theme	W-sol	1037:1041	arg1	Fr					1043:1044	W-sol Fr	1037:1044	W-sol Fr	1037:1044	Walls were isolated as an alcohol-insoluble residue (AIR) and sequentially extracted with hot water (W-sol Fr) and 1M KOH containing 1% NaBH4 (KOH-sol Fr).
24462668	7	114	theme	end	1555:1557	arg1	residue					1568:1574	the reducing end β-D-Xylp residue	1542:1574	the reducing end β-D-Xylp residue	1542:1574	Analysis of RE oligosaccharides, both 2AB labelled (from W-sol Fr) and glycosyl-alditol (from KOH-sol Fr), revealed that the RE glycosyl sequence of wheat endosperm AX comprises a linear (1→4)-β-D-Xylp backbone which may be mono-substituted with either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp.
24462668	4	115	theme	AXs	931:933	arg1	s					899:899	the RE oligosaccharide sequence(s)	867:900	the RE oligosaccharide sequence(s) of wheat endosperm cell wall AXs	867:933	Here we report the isolation and structural characterisation of the RE oligosaccharide sequence(s) of wheat endosperm cell wall AXs.
24462668	0	116	theme	reducing	4:11	arg1	sequence					17:24	The reducing end sequence	0:24	The reducing end sequence of wheat endosperm cell wall	0:53	The reducing end sequence of wheat endosperm cell wall arabinoxylans.
24462668	1	117	from	endosperm	110:118	arg1	Walls					70:74	Walls	70:74	Walls from wheat (Triticum aestivum L.) endosperm	70:118	Walls from wheat (Triticum aestivum L.) endosperm are composed primarily of hetero-(arabino)xylans (AXs) (70%) and (1→3)(1→4)-β-D-glucans (20%) with minor amounts of cellulose and heteromannans (2% each).
24462668	1	118	theme	1→4	191:193	arg1	-β-D-glucans					195:206	(1→3)(1→4)-β-D-glucans	185:206	(1→3)(1→4)-β-D-glucans (20%)	185:212	Walls from wheat (Triticum aestivum L.) endosperm are composed primarily of hetero-(arabino)xylans (AXs) (70%) and (1→3)(1→4)-β-D-glucans (20%) with minor amounts of cellulose and heteromannans (2% each).
24462668	1	118	theme	1→4	191:193	arg1	%					211:211	20%	209:211	20%	209:211	Walls from wheat (Triticum aestivum L.) endosperm are composed primarily of hetero-(arabino)xylans (AXs) (70%) and (1→3)(1→4)-β-D-glucans (20%) with minor amounts of cellulose and heteromannans (2% each).
24462668	7	119	theme	residue	1531:1537	arg1	-β-D-Xylp					1833:1841	either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp	1512:1841	either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp	1512:1841	Analysis of RE oligosaccharides, both 2AB labelled (from W-sol Fr) and glycosyl-alditol (from KOH-sol Fr), revealed that the RE glycosyl sequence of wheat endosperm AX comprises a linear (1→4)-β-D-Xylp backbone which may be mono-substituted with either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp.
24462668	2	120	theme	differential	293:304	arg1	properties					317:326	the differential solubility properties	289:326	the differential solubility properties of the AXs	289:337	To understand the differential solubility properties of the AXs, as well as aspects of their biosynthesis, we are sequencing the xylan backbone and examining the reducing end (RE) sequence(s) of wheat (monocot) AXs.
24462668	7	121	theme	1→4	1774:1776	arg1	-[α-L-Araf-					1778:1788	β-D-Xylp-(1→4)-[α-L-Araf-(1→3)	1764:1793	β-D-Xylp-(1→4)-[α-L-Araf-(1→3)	1764:1793	Analysis of RE oligosaccharides, both 2AB labelled (from W-sol Fr) and glycosyl-alditol (from KOH-sol Fr), revealed that the RE glycosyl sequence of wheat endosperm AX comprises a linear (1→4)-β-D-Xylp backbone which may be mono-substituted with either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp.
24462668	6	122	theme	Detailed	1092:1099	arg1	analysis					1112:1119	Detailed structural analysis	1092:1119	Detailed structural analysis of the RE oligosaccharides	1092:1146	Detailed structural analysis of the RE oligosaccharides was performed using a combination of methylation analysis, MALDI-TOF-MS, ESI-QTOF-MS, ESI-MS(n) and enzymic analysis.
24462668	1	123	with	xylans	162:167	arg1	amounts					225:231	minor amounts	219:231	minor amounts of cellulose	219:244	Walls from wheat (Triticum aestivum L.) endosperm are composed primarily of hetero-(arabino)xylans (AXs) (70%) and (1→3)(1→4)-β-D-glucans (20%) with minor amounts of cellulose and heteromannans (2% each).
24462668	1	123	with	xylans	162:167	arg1	heteromannans					250:262	heteromannans	250:262	heteromannans (2% each)	250:272	Walls from wheat (Triticum aestivum L.) endosperm are composed primarily of hetero-(arabino)xylans (AXs) (70%) and (1→3)(1→4)-β-D-glucans (20%) with minor amounts of cellulose and heteromannans (2% each).
24462668	1	123	with	xylans	162:167	arg1	cellulose					236:244	cellulose	236:244	cellulose	236:244	Walls from wheat (Triticum aestivum L.) endosperm are composed primarily of hetero-(arabino)xylans (AXs) (70%) and (1→3)(1→4)-β-D-glucans (20%) with minor amounts of cellulose and heteromannans (2% each).
24462668	7	124	theme	W-sol	1323:1327	arg1	Fr					1329:1330	W-sol Fr	1323:1330	W-sol Fr	1323:1330	Analysis of RE oligosaccharides, both 2AB labelled (from W-sol Fr) and glycosyl-alditol (from KOH-sol Fr), revealed that the RE glycosyl sequence of wheat endosperm AX comprises a linear (1→4)-β-D-Xylp backbone which may be mono-substituted with either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp.
24462668	7	124	theme	W-sol	1323:1327	arg1	2AB					1304:1306	both 2AB	1299:1306	both 2AB labelled (from W-sol Fr)	1299:1331	Analysis of RE oligosaccharides, both 2AB labelled (from W-sol Fr) and glycosyl-alditol (from KOH-sol Fr), revealed that the RE glycosyl sequence of wheat endosperm AX comprises a linear (1→4)-β-D-Xylp backbone which may be mono-substituted with either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp.
24462668	1	125	dep	heteromannans	250:262	arg1	%					266:266	2%	265:266	2% each	265:271	Walls from wheat (Triticum aestivum L.) endosperm are composed primarily of hetero-(arabino)xylans (AXs) (70%) and (1→3)(1→4)-β-D-glucans (20%) with minor amounts of cellulose and heteromannans (2% each).
24462668	7	126	theme	β-D-Xylp-	1764:1772	arg1	-[α-L-Araf-					1778:1788	β-D-Xylp-(1→4)-[α-L-Araf-(1→3)	1764:1793	β-D-Xylp-(1→4)-[α-L-Araf-(1→3)	1764:1793	Analysis of RE oligosaccharides, both 2AB labelled (from W-sol Fr) and glycosyl-alditol (from KOH-sol Fr), revealed that the RE glycosyl sequence of wheat endosperm AX comprises a linear (1→4)-β-D-Xylp backbone which may be mono-substituted with either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp.
24462668	5	127	theme	%	1070:1070	arg1	NaBH4					1072:1076	1% NaBH4	1069:1076	1% NaBH4 (KOH-sol Fr)	1069:1089	Walls were isolated as an alcohol-insoluble residue (AIR) and sequentially extracted with hot water (W-sol Fr) and 1M KOH containing 1% NaBH4 (KOH-sol Fr).
24462668	5	127	theme	%	1070:1070	arg1	Fr					1087:1088	KOH-sol Fr	1079:1088	KOH-sol Fr	1079:1088	Walls were isolated as an alcohol-insoluble residue (AIR) and sequentially extracted with hot water (W-sol Fr) and 1M KOH containing 1% NaBH4 (KOH-sol Fr).
24462668	0	128	theme	cell	45:48	arg1	wall					50:53	wheat endosperm cell wall	29:53	wheat endosperm cell wall	29:53	The reducing end sequence of wheat endosperm cell wall arabinoxylans.
24462668	3	129	dep	AXs	517:519	arg1	Miscanthus					555:564	Miscanthus	555:564	Miscanthus	555:564	A previous study of grass AXs (switchgrass, rice, Brachypodium, Miscanthus and foxtail millet) concluded that grasses lacked the comparable RE glycosyl sequence (4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp) found in dicots and gymnosperms but the actual RE sequence was not determined.
24462668	3	129	dep	AXs	517:519	arg1	millet					578:583	foxtail millet	570:583	foxtail millet	570:583	A previous study of grass AXs (switchgrass, rice, Brachypodium, Miscanthus and foxtail millet) concluded that grasses lacked the comparable RE glycosyl sequence (4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp) found in dicots and gymnosperms but the actual RE sequence was not determined.
24462668	3	129	dep	AXs	517:519	arg1	rice					535:538	rice	535:538	rice	535:538	A previous study of grass AXs (switchgrass, rice, Brachypodium, Miscanthus and foxtail millet) concluded that grasses lacked the comparable RE glycosyl sequence (4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp) found in dicots and gymnosperms but the actual RE sequence was not determined.
24462668	3	129	dep	AXs	517:519	arg1	AXs					517:519	grass AXs	511:519	grass AXs (switchgrass, rice, Brachypodium, Miscanthus and foxtail millet)	511:584	A previous study of grass AXs (switchgrass, rice, Brachypodium, Miscanthus and foxtail millet) concluded that grasses lacked the comparable RE glycosyl sequence (4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp) found in dicots and gymnosperms but the actual RE sequence was not determined.
24462668	3	129	dep	AXs	517:519	arg1	switchgrass					522:532	switchgrass	522:532	switchgrass	522:532	A previous study of grass AXs (switchgrass, rice, Brachypodium, Miscanthus and foxtail millet) concluded that grasses lacked the comparable RE glycosyl sequence (4-β-D-Xylp-(1→4)-β-D-Xylp-(1→3)-α-L-Rhap-(1→2)-α-D-GalpA-(1→4)-D-Xylp) found in dicots and gymnosperms but the actual RE sequence was not determined.
24462668	7	130	theme	β-D-Xylp	1559:1566	arg1	residue					1568:1574	the reducing end β-D-Xylp residue	1542:1574	the reducing end β-D-Xylp residue	1542:1574	Analysis of RE oligosaccharides, both 2AB labelled (from W-sol Fr) and glycosyl-alditol (from KOH-sol Fr), revealed that the RE glycosyl sequence of wheat endosperm AX comprises a linear (1→4)-β-D-Xylp backbone which may be mono-substituted with either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp.
24462668	7	131	theme	end	1742:1744	arg1	residue					1755:1761	the reducing end β-D-Xylp residue	1729:1761	the reducing end β-D-Xylp residue	1729:1761	Analysis of RE oligosaccharides, both 2AB labelled (from W-sol Fr) and glycosyl-alditol (from KOH-sol Fr), revealed that the RE glycosyl sequence of wheat endosperm AX comprises a linear (1→4)-β-D-Xylp backbone which may be mono-substituted with either an α-L-Araf residue at the reducing end β-D-Xylp residue and/or penultimate RE β-D-Xyl residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp and/or an α-D-GlcpA residue at the reducing end β-D-Xylp residue; β-D-Xylp-(1→4)-[α-L-Araf-(1→3)](+/-)-β-D-Xylp-(1→4)-[α-D-GlcAp-(1→2)]-β-D-Xylp.
24462668	5	132	theme	1M	1051:1052	arg1	KOH					1054:1056	1M KOH	1051:1056	1M KOH containing 1% NaBH4 (KOH-sol Fr)	1051:1089	Walls were isolated as an alcohol-insoluble residue (AIR) and sequentially extracted with hot water (W-sol Fr) and 1M KOH containing 1% NaBH4 (KOH-sol Fr).
24462668	5	133	contain	containing	1058:1067	arg2	Fr					1087:1088	KOH-sol Fr	1079:1088	KOH-sol Fr	1079:1088	Walls were isolated as an alcohol-insoluble residue (AIR) and sequentially extracted with hot water (W-sol Fr) and 1M KOH containing 1% NaBH4 (KOH-sol Fr).
24462668	5	133	contain	containing	1058:1067	arg2	NaBH4					1072:1076	1% NaBH4	1069:1076	1% NaBH4 (KOH-sol Fr)	1069:1089	Walls were isolated as an alcohol-insoluble residue (AIR) and sequentially extracted with hot water (W-sol Fr) and 1M KOH containing 1% NaBH4 (KOH-sol Fr).
24462668	5	133	contain	containing	1058:1067	arg1	KOH					1054:1056	1M KOH	1051:1056	1M KOH containing 1% NaBH4 (KOH-sol Fr)	1051:1089	Walls were isolated as an alcohol-insoluble residue (AIR) and sequentially extracted with hot water (W-sol Fr) and 1M KOH containing 1% NaBH4 (KOH-sol Fr).
27002546	2	0	theme	FODMAP	481:486	arg1	content					488:494	the FODMAP content	477:494	the FODMAP content of these commonly consumed foods	477:527	The aim was to identify and measure the FODMAP content of these commonly consumed foods.
27002546	3	1	theme	hexokinase	706:715	arg1	fructans					687:694	total fructans	681:694	total fructans	681:694	According to their perceived importance to clinical practise, the top 20 ranked foods underwent FODMAP analysis using validated analytical techniques (total fructans, Megazyme hexokinase (HK) assay; all others, high-performance liquid chromatography (HPLC) with evaporative light scattering detectors).
27002546	3	1	theme	hexokinase	706:715	arg1	assay					722:726	Megazyme hexokinase (HK) assay	697:726	Megazyme hexokinase (HK) assay	697:726	According to their perceived importance to clinical practise, the top 20 ranked foods underwent FODMAP analysis using validated analytical techniques (total fructans, Megazyme hexokinase (HK) assay; all others, high-performance liquid chromatography (HPLC) with evaporative light scattering detectors).
27002546	1	2	theme	Dietary	153:159	arg1	approach					286:293	an effective management approach	262:293	an effective management approach for functional bowel disorders	262:324	Dietary restriction of fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAPs) is an effective management approach for functional bowel disorders; however, its application is limited by the paucity of food composition data available for ethnic minority groups.
27002546	1	2	theme	Dietary	153:159	arg1	restriction					161:171	Dietary restriction	153:171	Dietary restriction of fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAPs)	153:257	Dietary restriction of fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAPs) is an effective management approach for functional bowel disorders; however, its application is limited by the paucity of food composition data available for ethnic minority groups.
27002546	0	3	theme	foods	80:84	arg1	content					69:75	Fermentable oligosaccharide, disaccharide, monosaccharide and polyol content	0:75	content	69:75	Fermentable oligosaccharide, disaccharide, monosaccharide and polyol content of foods commonly consumed by ethnic minority groups in the United Kingdom.
27002546	5	4	dep	detected	1126:1133	arg1	not					1122:1124	not	1122:1124	not	1122:1124	Fructans and galacto-oligosaccharides were the major FODMAPs in these foods, including channa dal (0.13 g/100 g; 0.36 g/100 g), fenugreek seeds (1.11 g/100 g; 1.27 g/100 g), guava (0.41 g/100 g; not detected), karela (not detected; 1.12 g/100 g) and tamarind (2.35 g/100 g; 0.02 g/100 g).
27002546	3	5	theme	analytical	658:667	arg1	techniques					669:678	validated analytical techniques	648:678	validated analytical techniques (total fructans, Megazyme hexokinase (HK) assay; all others, high-performance liquid chromatography (HPLC) with evaporative light scattering detectors)	648:830	According to their perceived importance to clinical practise, the top 20 ranked foods underwent FODMAP analysis using validated analytical techniques (total fructans, Megazyme hexokinase (HK) assay; all others, high-performance liquid chromatography (HPLC) with evaporative light scattering detectors).
27002546	5	6	theme	fenugreek	1055:1063	arg1	seeds					1065:1069	fenugreek seeds	1055:1069	fenugreek seeds (1.11 g/100 g; 1.27 g/100 g)	1055:1098	Fructans and galacto-oligosaccharides were the major FODMAPs in these foods, including channa dal (0.13 g/100 g; 0.36 g/100 g), fenugreek seeds (1.11 g/100 g; 1.27 g/100 g), guava (0.41 g/100 g; not detected), karela (not detected; 1.12 g/100 g) and tamarind (2.35 g/100 g; 0.02 g/100 g).
27002546	5	6	theme	fenugreek	1055:1063	arg1	1.27 g/100 g					1086:1097	1.27 g/100 g	1086:1097	1.27 g/100 g	1086:1097	Fructans and galacto-oligosaccharides were the major FODMAPs in these foods, including channa dal (0.13 g/100 g; 0.36 g/100 g), fenugreek seeds (1.11 g/100 g; 1.27 g/100 g), guava (0.41 g/100 g; not detected), karela (not detected; 1.12 g/100 g) and tamarind (2.35 g/100 g; 0.02 g/100 g).
27002546	3	7	dep	techniques	669:678	arg1	fructans					687:694	total fructans	681:694	total fructans	681:694	According to their perceived importance to clinical practise, the top 20 ranked foods underwent FODMAP analysis using validated analytical techniques (total fructans, Megazyme hexokinase (HK) assay; all others, high-performance liquid chromatography (HPLC) with evaporative light scattering detectors).
27002546	3	7	dep	techniques	669:678	arg1	chromatography					765:778	high-performance liquid chromatography	741:778	high-performance liquid chromatography (HPLC) with evaporative light scattering detectors	741:829	According to their perceived importance to clinical practise, the top 20 ranked foods underwent FODMAP analysis using validated analytical techniques (total fructans, Megazyme hexokinase (HK) assay; all others, high-performance liquid chromatography (HPLC) with evaporative light scattering detectors).
27002546	3	7	dep	techniques	669:678	arg1	assay					722:726	Megazyme hexokinase (HK) assay	697:726	Megazyme hexokinase (HK) assay	697:726	According to their perceived importance to clinical practise, the top 20 ranked foods underwent FODMAP analysis using validated analytical techniques (total fructans, Megazyme hexokinase (HK) assay; all others, high-performance liquid chromatography (HPLC) with evaporative light scattering detectors).
27002546	2	8	theme	foods	523:527	arg1	content					488:494	the FODMAP content	477:494	the FODMAP content of these commonly consumed foods	477:527	The aim was to identify and measure the FODMAP content of these commonly consumed foods.
27002546	1	9	theme	effective	265:273	arg1	approach					286:293	an effective management approach	262:293	an effective management approach for functional bowel disorders	262:324	Dietary restriction of fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAPs) is an effective management approach for functional bowel disorders; however, its application is limited by the paucity of food composition data available for ethnic minority groups.
27002546	1	9	theme	effective	265:273	arg1	restriction					161:171	Dietary restriction	153:171	Dietary restriction of fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAPs)	153:257	Dietary restriction of fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAPs) is an effective management approach for functional bowel disorders; however, its application is limited by the paucity of food composition data available for ethnic minority groups.
27002546	2	10	theme	consumed	514:521	arg1	foods					523:527	these commonly consumed foods	499:527	these commonly consumed foods	499:527	The aim was to identify and measure the FODMAP content of these commonly consumed foods.
27002546	6	11	theme	low	1313:1315	arg1	advice					1332:1337	low FODMAP dietary advice	1313:1337	low FODMAP dietary advice	1313:1337	Broadening the availability of FODMAP composition data will increase the cultural application of low FODMAP dietary advice.
27002546	3	12	with	chromatography	765:778	arg1	detectors					821:829	evaporative light scattering detectors	792:829	evaporative light scattering detectors	792:829	According to their perceived importance to clinical practise, the top 20 ranked foods underwent FODMAP analysis using validated analytical techniques (total fructans, Megazyme hexokinase (HK) assay; all others, high-performance liquid chromatography (HPLC) with evaporative light scattering detectors).
27002546	1	13	theme	management	275:284	arg1	approach					286:293	an effective management approach	262:293	an effective management approach for functional bowel disorders	262:324	Dietary restriction of fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAPs) is an effective management approach for functional bowel disorders; however, its application is limited by the paucity of food composition data available for ethnic minority groups.
27002546	1	13	theme	management	275:284	arg1	restriction					161:171	Dietary restriction	153:171	Dietary restriction of fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAPs)	153:257	Dietary restriction of fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAPs) is an effective management approach for functional bowel disorders; however, its application is limited by the paucity of food composition data available for ethnic minority groups.
27002546	0	14	theme	minority	114:121	arg1	groups					123:128	ethnic minority groups	107:128	ethnic minority groups in the United Kingdom	107:150	Fermentable oligosaccharide, disaccharide, monosaccharide and polyol content of foods commonly consumed by ethnic minority groups in the United Kingdom.
27002546	6	15	theme	dietary	1324:1330	arg1	advice					1332:1337	low FODMAP dietary advice	1313:1337	low FODMAP dietary advice	1313:1337	Broadening the availability of FODMAP composition data will increase the cultural application of low FODMAP dietary advice.
27002546	0	16	theme	ethnic	107:112	arg1	groups					123:128	ethnic minority groups	107:128	ethnic minority groups in the United Kingdom	107:150	Fermentable oligosaccharide, disaccharide, monosaccharide and polyol content of foods commonly consumed by ethnic minority groups in the United Kingdom.
27002546	6	17	theme	Broadening	1216:1225	arg1	availability					1231:1242	the availability	1227:1242	Broadening the availability of FODMAP composition data	1216:1269	Broadening the availability of FODMAP composition data will increase the cultural application of low FODMAP dietary advice.
27002546	1	18	theme	fermentable	176:186	arg1	oligosaccharides					188:203	fermentable oligosaccharides	176:203	fermentable oligosaccharides	176:203	Dietary restriction of fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAPs) is an effective management approach for functional bowel disorders; however, its application is limited by the paucity of food composition data available for ethnic minority groups.
27002546	1	18	theme	fermentable	176:186	arg1	FODMAPs					250:256	FODMAPs	250:256	FODMAPs	250:256	Dietary restriction of fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAPs) is an effective management approach for functional bowel disorders; however, its application is limited by the paucity of food composition data available for ethnic minority groups.
27002546	0	19	theme	oligosaccharide	12:26	arg1	content					69:75	Fermentable oligosaccharide, disaccharide, monosaccharide and polyol content	0:75	content	69:75	Fermentable oligosaccharide, disaccharide, monosaccharide and polyol content of foods commonly consumed by ethnic minority groups in the United Kingdom.
27002546	6	20	theme	FODMAP	1317:1322	arg1	advice					1332:1337	low FODMAP dietary advice	1313:1337	low FODMAP dietary advice	1313:1337	Broadening the availability of FODMAP composition data will increase the cultural application of low FODMAP dietary advice.
27002546	5	21	from	FODMAPs	980:986	arg1	foods					997:1001	these foods	991:1001	these foods	991:1001	Fructans and galacto-oligosaccharides were the major FODMAPs in these foods, including channa dal (0.13 g/100 g; 0.36 g/100 g), fenugreek seeds (1.11 g/100 g; 1.27 g/100 g), guava (0.41 g/100 g; not detected), karela (not detected; 1.12 g/100 g) and tamarind (2.35 g/100 g; 0.02 g/100 g).
27002546	1	22	theme	oligosaccharides	188:203	arg1	approach					286:293	an effective management approach	262:293	an effective management approach for functional bowel disorders	262:324	Dietary restriction of fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAPs) is an effective management approach for functional bowel disorders; however, its application is limited by the paucity of food composition data available for ethnic minority groups.
27002546	1	22	theme	oligosaccharides	188:203	arg1	restriction					161:171	Dietary restriction	153:171	Dietary restriction of fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAPs)	153:257	Dietary restriction of fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAPs) is an effective management approach for functional bowel disorders; however, its application is limited by the paucity of food composition data available for ethnic minority groups.
27002546	0	23	theme	Fermentable	0:10	arg1	content					69:75	Fermentable oligosaccharide, disaccharide, monosaccharide and polyol content	0:75	content	69:75	Fermentable oligosaccharide, disaccharide, monosaccharide and polyol content of foods commonly consumed by ethnic minority groups in the United Kingdom.
27002546	3	24	dep	fructans	687:694	arg1	others					733:738	all others	729:738	all others	729:738	According to their perceived importance to clinical practise, the top 20 ranked foods underwent FODMAP analysis using validated analytical techniques (total fructans, Megazyme hexokinase (HK) assay; all others, high-performance liquid chromatography (HPLC) with evaporative light scattering detectors).
27002546	3	25	theme	clinical	573:580	arg1	practise					582:589	clinical practise	573:589	clinical practise	573:589	According to their perceived importance to clinical practise, the top 20 ranked foods underwent FODMAP analysis using validated analytical techniques (total fructans, Megazyme hexokinase (HK) assay; all others, high-performance liquid chromatography (HPLC) with evaporative light scattering detectors).
27002546	3	26	theme	validated	648:656	arg1	techniques					669:678	validated analytical techniques	648:678	validated analytical techniques (total fructans, Megazyme hexokinase (HK) assay; all others, high-performance liquid chromatography (HPLC) with evaporative light scattering detectors)	648:830	According to their perceived importance to clinical practise, the top 20 ranked foods underwent FODMAP analysis using validated analytical techniques (total fructans, Megazyme hexokinase (HK) assay; all others, high-performance liquid chromatography (HPLC) with evaporative light scattering detectors).
27002546	0	27	theme	disaccharide	29:40	arg1	content					69:75	Fermentable oligosaccharide, disaccharide, monosaccharide and polyol content	0:75	content	69:75	Fermentable oligosaccharide, disaccharide, monosaccharide and polyol content of foods commonly consumed by ethnic minority groups in the United Kingdom.
27002546	5	28	dep	guava	1101:1105	arg1	detected					1126:1133	detected	1126:1133	detected	1126:1133	Fructans and galacto-oligosaccharides were the major FODMAPs in these foods, including channa dal (0.13 g/100 g; 0.36 g/100 g), fenugreek seeds (1.11 g/100 g; 1.27 g/100 g), guava (0.41 g/100 g; not detected), karela (not detected; 1.12 g/100 g) and tamarind (2.35 g/100 g; 0.02 g/100 g).
27002546	5	29	theme	channa	1014:1019	arg1	dal					1021:1023	channa dal	1014:1023	channa dal (0.13 g/100 g; 0.36 g/100 g)	1014:1052	Fructans and galacto-oligosaccharides were the major FODMAPs in these foods, including channa dal (0.13 g/100 g; 0.36 g/100 g), fenugreek seeds (1.11 g/100 g; 1.27 g/100 g), guava (0.41 g/100 g; not detected), karela (not detected; 1.12 g/100 g) and tamarind (2.35 g/100 g; 0.02 g/100 g).
27002546	5	29	theme	channa	1014:1019	arg1	0.36 g/100 g					1040:1051	0.36 g/100 g	1040:1051	0.36 g/100 g	1040:1051	Fructans and galacto-oligosaccharides were the major FODMAPs in these foods, including channa dal (0.13 g/100 g; 0.36 g/100 g), fenugreek seeds (1.11 g/100 g; 1.27 g/100 g), guava (0.41 g/100 g; not detected), karela (not detected; 1.12 g/100 g) and tamarind (2.35 g/100 g; 0.02 g/100 g).
27002546	3	30	theme	scattering	810:819	arg1	detectors					821:829	evaporative light scattering detectors	792:829	evaporative light scattering detectors	792:829	According to their perceived importance to clinical practise, the top 20 ranked foods underwent FODMAP analysis using validated analytical techniques (total fructans, Megazyme hexokinase (HK) assay; all others, high-performance liquid chromatography (HPLC) with evaporative light scattering detectors).
27002546	1	31	theme	disaccharides	206:218	arg1	approach					286:293	an effective management approach	262:293	an effective management approach for functional bowel disorders	262:324	Dietary restriction of fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAPs) is an effective management approach for functional bowel disorders; however, its application is limited by the paucity of food composition data available for ethnic minority groups.
27002546	1	31	theme	disaccharides	206:218	arg1	restriction					161:171	Dietary restriction	153:171	Dietary restriction of fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAPs)	153:257	Dietary restriction of fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAPs) is an effective management approach for functional bowel disorders; however, its application is limited by the paucity of food composition data available for ethnic minority groups.
27002546	1	32	theme	functional	299:308	arg1	disorders					316:324	functional bowel disorders	299:324	functional bowel disorders	299:324	Dietary restriction of fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAPs) is an effective management approach for functional bowel disorders; however, its application is limited by the paucity of food composition data available for ethnic minority groups.
27002546	1	33	theme	food	381:384	arg1	data					398:401	food composition data	381:401	food composition data available for ethnic minority groups	381:438	Dietary restriction of fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAPs) is an effective management approach for functional bowel disorders; however, its application is limited by the paucity of food composition data available for ethnic minority groups.
27002546	0	34	theme	monosaccharide	43:56	arg1	content					69:75	Fermentable oligosaccharide, disaccharide, monosaccharide and polyol content	0:75	content	69:75	Fermentable oligosaccharide, disaccharide, monosaccharide and polyol content of foods commonly consumed by ethnic minority groups in the United Kingdom.
27002546	1	35	theme	bowel	310:314	arg1	disorders					316:324	functional bowel disorders	299:324	functional bowel disorders	299:324	Dietary restriction of fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAPs) is an effective management approach for functional bowel disorders; however, its application is limited by the paucity of food composition data available for ethnic minority groups.
27002546	0	36	theme	United	137:142	arg1	Kingdom					144:150	the United Kingdom	133:150	the United Kingdom	133:150	Fermentable oligosaccharide, disaccharide, monosaccharide and polyol content of foods commonly consumed by ethnic minority groups in the United Kingdom.
27002546	1	37	theme	composition	386:396	arg1	data					398:401	food composition data	381:401	food composition data available for ethnic minority groups	381:438	Dietary restriction of fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAPs) is an effective management approach for functional bowel disorders; however, its application is limited by the paucity of food composition data available for ethnic minority groups.
27002546	1	38	theme	monosaccharides	221:235	arg1	approach					286:293	an effective management approach	262:293	an effective management approach for functional bowel disorders	262:324	Dietary restriction of fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAPs) is an effective management approach for functional bowel disorders; however, its application is limited by the paucity of food composition data available for ethnic minority groups.
27002546	1	38	theme	monosaccharides	221:235	arg1	restriction					161:171	Dietary restriction	153:171	Dietary restriction of fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAPs)	153:257	Dietary restriction of fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAPs) is an effective management approach for functional bowel disorders; however, its application is limited by the paucity of food composition data available for ethnic minority groups.
27002546	6	39	theme	advice	1332:1337	arg1	application					1298:1308	the cultural application	1285:1308	the cultural application of low FODMAP dietary advice	1285:1337	Broadening the availability of FODMAP composition data will increase the cultural application of low FODMAP dietary advice.
27002546	6	40	theme	data	1266:1269	arg1	availability					1231:1242	the availability	1227:1242	Broadening the availability of FODMAP composition data	1216:1269	Broadening the availability of FODMAP composition data will increase the cultural application of low FODMAP dietary advice.
27002546	1	41	theme	data	398:401	arg1	paucity					370:376	the paucity	366:376	the paucity of food composition data available for ethnic minority groups	366:438	Dietary restriction of fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAPs) is an effective management approach for functional bowel disorders; however, its application is limited by the paucity of food composition data available for ethnic minority groups.
27002546	3	42	theme	top	596:598	arg1	foods					610:614	the top 20 ranked foods	592:614	the top 20 ranked foods	592:614	According to their perceived importance to clinical practise, the top 20 ranked foods underwent FODMAP analysis using validated analytical techniques (total fructans, Megazyme hexokinase (HK) assay; all others, high-performance liquid chromatography (HPLC) with evaporative light scattering detectors).
27002546	6	43	theme	cultural	1289:1296	arg1	application					1298:1308	the cultural application	1285:1308	the cultural application of low FODMAP dietary advice	1285:1337	Broadening the availability of FODMAP composition data will increase the cultural application of low FODMAP dietary advice.
27002546	0	44	theme	polyol	62:67	arg1	content					69:75	Fermentable oligosaccharide, disaccharide, monosaccharide and polyol content	0:75	content	69:75	Fermentable oligosaccharide, disaccharide, monosaccharide and polyol content of foods commonly consumed by ethnic minority groups in the United Kingdom.
27002546	4	45	theme	FODMAP	919:924	arg1	sources					895:901	significant sources	883:901	significant sources of at least one FODMAP	883:924	Of the 20 foods analysed, five were identified as significant sources of at least one FODMAP.
27002546	4	45	theme	FODMAP	919:924	arg1	five					859:862	five	859:862	five	859:862	Of the 20 foods analysed, five were identified as significant sources of at least one FODMAP.
27002546	6	46	theme	composition	1254:1264	arg1	data					1266:1269	FODMAP composition data	1247:1269	FODMAP composition data	1247:1269	Broadening the availability of FODMAP composition data will increase the cultural application of low FODMAP dietary advice.
27002546	1	47	theme	available	403:411	arg1	data					398:401	food composition data	381:401	food composition data available for ethnic minority groups	381:438	Dietary restriction of fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAPs) is an effective management approach for functional bowel disorders; however, its application is limited by the paucity of food composition data available for ethnic minority groups.
27002546	5	48	dep	karela	1137:1142	arg1	detected					1149:1156	detected	1149:1156	detected; 1.12 g/100 g	1149:1170	Fructans and galacto-oligosaccharides were the major FODMAPs in these foods, including channa dal (0.13 g/100 g; 0.36 g/100 g), fenugreek seeds (1.11 g/100 g; 1.27 g/100 g), guava (0.41 g/100 g; not detected), karela (not detected; 1.12 g/100 g) and tamarind (2.35 g/100 g; 0.02 g/100 g).
27002546	3	49	theme	high-performance	741:756	arg1	HPLC					781:784	HPLC	781:784	HPLC	781:784	According to their perceived importance to clinical practise, the top 20 ranked foods underwent FODMAP analysis using validated analytical techniques (total fructans, Megazyme hexokinase (HK) assay; all others, high-performance liquid chromatography (HPLC) with evaporative light scattering detectors).
27002546	3	49	theme	high-performance	741:756	arg1	chromatography					765:778	high-performance liquid chromatography	741:778	high-performance liquid chromatography (HPLC) with evaporative light scattering detectors	741:829	According to their perceived importance to clinical practise, the top 20 ranked foods underwent FODMAP analysis using validated analytical techniques (total fructans, Megazyme hexokinase (HK) assay; all others, high-performance liquid chromatography (HPLC) with evaporative light scattering detectors).
27002546	3	49	theme	high-performance	741:756	arg1	fructans					687:694	total fructans	681:694	total fructans	681:694	According to their perceived importance to clinical practise, the top 20 ranked foods underwent FODMAP analysis using validated analytical techniques (total fructans, Megazyme hexokinase (HK) assay; all others, high-performance liquid chromatography (HPLC) with evaporative light scattering detectors).
27002546	6	50	theme	FODMAP	1247:1252	arg1	data					1266:1269	FODMAP composition data	1247:1269	FODMAP composition data	1247:1269	Broadening the availability of FODMAP composition data will increase the cultural application of low FODMAP dietary advice.
27002546	5	51	dep	detected	1149:1156	arg1	not					1145:1147	not	1145:1147	not	1145:1147	Fructans and galacto-oligosaccharides were the major FODMAPs in these foods, including channa dal (0.13 g/100 g; 0.36 g/100 g), fenugreek seeds (1.11 g/100 g; 1.27 g/100 g), guava (0.41 g/100 g; not detected), karela (not detected; 1.12 g/100 g) and tamarind (2.35 g/100 g; 0.02 g/100 g).
27002546	3	52	theme	total	681:685	arg1	fructans					687:694	total fructans	681:694	total fructans	681:694	According to their perceived importance to clinical practise, the top 20 ranked foods underwent FODMAP analysis using validated analytical techniques (total fructans, Megazyme hexokinase (HK) assay; all others, high-performance liquid chromatography (HPLC) with evaporative light scattering detectors).
27002546	3	52	theme	total	681:685	arg1	chromatography					765:778	high-performance liquid chromatography	741:778	high-performance liquid chromatography (HPLC) with evaporative light scattering detectors	741:829	According to their perceived importance to clinical practise, the top 20 ranked foods underwent FODMAP analysis using validated analytical techniques (total fructans, Megazyme hexokinase (HK) assay; all others, high-performance liquid chromatography (HPLC) with evaporative light scattering detectors).
27002546	3	52	theme	total	681:685	arg1	assay					722:726	Megazyme hexokinase (HK) assay	697:726	Megazyme hexokinase (HK) assay	697:726	According to their perceived importance to clinical practise, the top 20 ranked foods underwent FODMAP analysis using validated analytical techniques (total fructans, Megazyme hexokinase (HK) assay; all others, high-performance liquid chromatography (HPLC) with evaporative light scattering detectors).
27002546	3	53	theme	FODMAP	626:631	arg1	analysis					633:640	FODMAP analysis	626:640	FODMAP analysis	626:640	According to their perceived importance to clinical practise, the top 20 ranked foods underwent FODMAP analysis using validated analytical techniques (total fructans, Megazyme hexokinase (HK) assay; all others, high-performance liquid chromatography (HPLC) with evaporative light scattering detectors).
27002546	3	54	theme	liquid	758:763	arg1	HPLC					781:784	HPLC	781:784	HPLC	781:784	According to their perceived importance to clinical practise, the top 20 ranked foods underwent FODMAP analysis using validated analytical techniques (total fructans, Megazyme hexokinase (HK) assay; all others, high-performance liquid chromatography (HPLC) with evaporative light scattering detectors).
27002546	3	54	theme	liquid	758:763	arg1	chromatography					765:778	high-performance liquid chromatography	741:778	high-performance liquid chromatography (HPLC) with evaporative light scattering detectors	741:829	According to their perceived importance to clinical practise, the top 20 ranked foods underwent FODMAP analysis using validated analytical techniques (total fructans, Megazyme hexokinase (HK) assay; all others, high-performance liquid chromatography (HPLC) with evaporative light scattering detectors).
27002546	3	54	theme	liquid	758:763	arg1	fructans					687:694	total fructans	681:694	total fructans	681:694	According to their perceived importance to clinical practise, the top 20 ranked foods underwent FODMAP analysis using validated analytical techniques (total fructans, Megazyme hexokinase (HK) assay; all others, high-performance liquid chromatography (HPLC) with evaporative light scattering detectors).
27002546	4	55	theme	significant	883:893	arg1	sources					895:901	significant sources	883:901	significant sources of at least one FODMAP	883:924	Of the 20 foods analysed, five were identified as significant sources of at least one FODMAP.
27002546	4	55	theme	significant	883:893	arg1	five					859:862	five	859:862	five	859:862	Of the 20 foods analysed, five were identified as significant sources of at least one FODMAP.
27002546	1	56	theme	ethnic	417:422	arg1	groups					433:438	ethnic minority groups	417:438	ethnic minority groups	417:438	Dietary restriction of fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAPs) is an effective management approach for functional bowel disorders; however, its application is limited by the paucity of food composition data available for ethnic minority groups.
27002546	5	57	theme	major	974:978	arg1	dal					1021:1023	channa dal	1014:1023	channa dal (0.13 g/100 g; 0.36 g/100 g)	1014:1052	Fructans and galacto-oligosaccharides were the major FODMAPs in these foods, including channa dal (0.13 g/100 g; 0.36 g/100 g), fenugreek seeds (1.11 g/100 g; 1.27 g/100 g), guava (0.41 g/100 g; not detected), karela (not detected; 1.12 g/100 g) and tamarind (2.35 g/100 g; 0.02 g/100 g).
27002546	5	57	theme	major	974:978	arg1	guava					1101:1105	guava	1101:1105	guava (0.41 g/100 g; not detected)	1101:1134	Fructans and galacto-oligosaccharides were the major FODMAPs in these foods, including channa dal (0.13 g/100 g; 0.36 g/100 g), fenugreek seeds (1.11 g/100 g; 1.27 g/100 g), guava (0.41 g/100 g; not detected), karela (not detected; 1.12 g/100 g) and tamarind (2.35 g/100 g; 0.02 g/100 g).
27002546	5	57	theme	major	974:978	arg1	galacto-oligosaccharides					940:963	galacto-oligosaccharides	940:963	galacto-oligosaccharides	940:963	Fructans and galacto-oligosaccharides were the major FODMAPs in these foods, including channa dal (0.13 g/100 g; 0.36 g/100 g), fenugreek seeds (1.11 g/100 g; 1.27 g/100 g), guava (0.41 g/100 g; not detected), karela (not detected; 1.12 g/100 g) and tamarind (2.35 g/100 g; 0.02 g/100 g).
27002546	5	57	theme	major	974:978	arg1	seeds					1065:1069	fenugreek seeds	1055:1069	fenugreek seeds (1.11 g/100 g; 1.27 g/100 g)	1055:1098	Fructans and galacto-oligosaccharides were the major FODMAPs in these foods, including channa dal (0.13 g/100 g; 0.36 g/100 g), fenugreek seeds (1.11 g/100 g; 1.27 g/100 g), guava (0.41 g/100 g; not detected), karela (not detected; 1.12 g/100 g) and tamarind (2.35 g/100 g; 0.02 g/100 g).
27002546	5	57	theme	major	974:978	arg1	karela					1137:1142	karela	1137:1142	karela (not detected; 1.12 g/100 g)	1137:1171	Fructans and galacto-oligosaccharides were the major FODMAPs in these foods, including channa dal (0.13 g/100 g; 0.36 g/100 g), fenugreek seeds (1.11 g/100 g; 1.27 g/100 g), guava (0.41 g/100 g; not detected), karela (not detected; 1.12 g/100 g) and tamarind (2.35 g/100 g; 0.02 g/100 g).
27002546	5	57	theme	major	974:978	arg1	tamarind					1177:1184	tamarind	1177:1184	tamarind	1177:1184	Fructans and galacto-oligosaccharides were the major FODMAPs in these foods, including channa dal (0.13 g/100 g; 0.36 g/100 g), fenugreek seeds (1.11 g/100 g; 1.27 g/100 g), guava (0.41 g/100 g; not detected), karela (not detected; 1.12 g/100 g) and tamarind (2.35 g/100 g; 0.02 g/100 g).
27002546	5	57	theme	major	974:978	arg1	Fructans					927:934	Fructans	927:934	Fructans	927:934	Fructans and galacto-oligosaccharides were the major FODMAPs in these foods, including channa dal (0.13 g/100 g; 0.36 g/100 g), fenugreek seeds (1.11 g/100 g; 1.27 g/100 g), guava (0.41 g/100 g; not detected), karela (not detected; 1.12 g/100 g) and tamarind (2.35 g/100 g; 0.02 g/100 g).
27002546	5	57	theme	major	974:978	arg1	FODMAPs					980:986	the major FODMAPs	970:986	the major FODMAPs	970:986	Fructans and galacto-oligosaccharides were the major FODMAPs in these foods, including channa dal (0.13 g/100 g; 0.36 g/100 g), fenugreek seeds (1.11 g/100 g; 1.27 g/100 g), guava (0.41 g/100 g; not detected), karela (not detected; 1.12 g/100 g) and tamarind (2.35 g/100 g; 0.02 g/100 g).
27002546	3	58	theme	perceived	549:557	arg1	importance					559:568	their perceived importance	543:568	their perceived importance to clinical practise	543:589	According to their perceived importance to clinical practise, the top 20 ranked foods underwent FODMAP analysis using validated analytical techniques (total fructans, Megazyme hexokinase (HK) assay; all others, high-performance liquid chromatography (HPLC) with evaporative light scattering detectors).
27002546	1	59	theme	polyols	241:247	arg1	approach					286:293	an effective management approach	262:293	an effective management approach for functional bowel disorders	262:324	Dietary restriction of fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAPs) is an effective management approach for functional bowel disorders; however, its application is limited by the paucity of food composition data available for ethnic minority groups.
27002546	1	59	theme	polyols	241:247	arg1	restriction					161:171	Dietary restriction	153:171	Dietary restriction of fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAPs)	153:257	Dietary restriction of fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAPs) is an effective management approach for functional bowel disorders; however, its application is limited by the paucity of food composition data available for ethnic minority groups.
27002546	1	60	theme	minority	424:431	arg1	groups					433:438	ethnic minority groups	417:438	ethnic minority groups	417:438	Dietary restriction of fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAPs) is an effective management approach for functional bowel disorders; however, its application is limited by the paucity of food composition data available for ethnic minority groups.
27002546	3	61	theme	evaporative	792:802	arg1	detectors					821:829	evaporative light scattering detectors	792:829	evaporative light scattering detectors	792:829	According to their perceived importance to clinical practise, the top 20 ranked foods underwent FODMAP analysis using validated analytical techniques (total fructans, Megazyme hexokinase (HK) assay; all others, high-performance liquid chromatography (HPLC) with evaporative light scattering detectors).
27002546	3	62	theme	light	804:808	arg1	detectors					821:829	evaporative light scattering detectors	792:829	evaporative light scattering detectors	792:829	According to their perceived importance to clinical practise, the top 20 ranked foods underwent FODMAP analysis using validated analytical techniques (total fructans, Megazyme hexokinase (HK) assay; all others, high-performance liquid chromatography (HPLC) with evaporative light scattering detectors).
27002546	3	63	theme	Megazyme	697:704	arg1	HK					718:719	HK	718:719	HK	718:719	According to their perceived importance to clinical practise, the top 20 ranked foods underwent FODMAP analysis using validated analytical techniques (total fructans, Megazyme hexokinase (HK) assay; all others, high-performance liquid chromatography (HPLC) with evaporative light scattering detectors).
27002546	3	63	theme	Megazyme	697:704	arg1	hexokinase					706:715	Megazyme hexokinase	697:715	Megazyme hexokinase (HK) assay	697:726	According to their perceived importance to clinical practise, the top 20 ranked foods underwent FODMAP analysis using validated analytical techniques (total fructans, Megazyme hexokinase (HK) assay; all others, high-performance liquid chromatography (HPLC) with evaporative light scattering detectors).
27002546	3	64	theme	ranked	603:608	arg1	foods					610:614	the top 20 ranked foods	592:614	the top 20 ranked foods	592:614	According to their perceived importance to clinical practise, the top 20 ranked foods underwent FODMAP analysis using validated analytical techniques (total fructans, Megazyme hexokinase (HK) assay; all others, high-performance liquid chromatography (HPLC) with evaporative light scattering detectors).
27002546	0	65	from	groups	123:128	arg1	Kingdom					144:150	the United Kingdom	133:150	the United Kingdom	133:150	Fermentable oligosaccharide, disaccharide, monosaccharide and polyol content of foods commonly consumed by ethnic minority groups in the United Kingdom.
26486518	0	0	theme	Burkholderia	103:114	arg1	bpsI					129:132	Burkholderia pseudomallei bpsI	103:132	Burkholderia pseudomallei bpsI	103:132	Correlation between biofilm production, antibiotic susceptibility and exopolysaccharide composition in Burkholderia pseudomallei bpsI, ppk, and rpoS mutant strains.
26486518	7	1	theme	mutants	1127:1133	arg1	exopolysaccharides					1101:1118	the exopolysaccharides	1097:1118	the exopolysaccharides of the mutants	1097:1133	Different ratios of carbohydrate contents in the exopolysaccharides of the mutants were detected, although they have the same components, including glucose, galactose, mannose, and rhamnose, with the exception being that no detectable rhamnose peak was observed in the bpsI mutant.
26486518	1	2	theme	Burkholderia	165:176	arg1	cause					198:202	the cause	194:202	the cause of melioidosis, a fatal tropical infectious disease, which has been reported to have a high rate of recurrence, even when an intensive dose of antibiotics is used	194:365	Burkholderia pseudomallei is the cause of melioidosis, a fatal tropical infectious disease, which has been reported to have a high rate of recurrence, even when an intensive dose of antibiotics is used.
26486518	1	2	theme	Burkholderia	165:176	arg1	pseudomallei					178:189	Burkholderia pseudomallei	165:189	Burkholderia pseudomallei	165:189	Burkholderia pseudomallei is the cause of melioidosis, a fatal tropical infectious disease, which has been reported to have a high rate of recurrence, even when an intensive dose of antibiotics is used.
26486518	5	3	theme	mutant	870:875	arg1	strains					877:883	rpoS mutant strains	865:883	rpoS mutant strains	865:883	The present study presents a correlation between exopolysaccharide production in biofilm matrix and antibiotic resistance in B. pseudomallei using bpsI, ppk, and rpoS mutant strains.
26486518	6	4	theme	B.	983:984	arg1	mutants					999:1005	B. pseudomallei mutants	983:1005	B. pseudomallei mutants	983:1005	CLSM revealed a reduction in exopolysaccharide production and disabled micro-colony formation in B. pseudomallei mutants, which paralleled the antibiotic resistance.
26486518	1	5	theme	intensive	329:337	arg1	dose					339:342	an intensive dose	326:342	an intensive dose of antibiotics	326:357	Burkholderia pseudomallei is the cause of melioidosis, a fatal tropical infectious disease, which has been reported to have a high rate of recurrence, even when an intensive dose of antibiotics is used.
26486518	3	6	from	pseudomallei	593:604	arg1	limitation					550:559	the limitation	546:559	the limitation of antibiotic penetration in B. pseudomallei	546:604	EPS in biofilms have been reported to be related to the limitation of antibiotic penetration in B. pseudomallei.
26486518	8	7	from	correlation	1366:1376	arg1	biofilm					1425:1431	the B. pseudomallei biofilm	1405:1431	the B. pseudomallei biofilm at least	1405:1440	These results indicate that the correlation between these phenomena in the B. pseudomallei biofilm at least results from the exopolysaccharide, which may be under the regulation of bpsI, ppk, or rpoS genes.
26486518	5	8	theme	rpoS	865:868	arg1	strains					877:883	rpoS mutant strains	865:883	rpoS mutant strains	865:883	The present study presents a correlation between exopolysaccharide production in biofilm matrix and antibiotic resistance in B. pseudomallei using bpsI, ppk, and rpoS mutant strains.
26486518	1	9	used	used	362:365	arg2	dose					339:342	an intensive dose	326:342	an intensive dose of antibiotics	326:357	Burkholderia pseudomallei is the cause of melioidosis, a fatal tropical infectious disease, which has been reported to have a high rate of recurrence, even when an intensive dose of antibiotics is used.
26486518	2	10	theme	drug	477:480	arg1	resistance					482:491	drug resistance	477:491	drug resistance	477:491	Biofilm formation is believed to be one of the possible causes of relapse because of its ability to increase drug resistance.
26486518	1	11	contain	have	284:287	arg1	disease					248:254	a fatal tropical infectious disease	220:254	a fatal tropical infectious disease	220:254	Burkholderia pseudomallei is the cause of melioidosis, a fatal tropical infectious disease, which has been reported to have a high rate of recurrence, even when an intensive dose of antibiotics is used.
26486518	1	11	contain	have	284:287	arg2	rate					296:299	a high rate	289:299	a high rate of recurrence	289:313	Burkholderia pseudomallei is the cause of melioidosis, a fatal tropical infectious disease, which has been reported to have a high rate of recurrence, even when an intensive dose of antibiotics is used.
26486518	1	11	contain	have	284:287	arg1	melioidosis					207:217	melioidosis	207:217	melioidosis	207:217	Burkholderia pseudomallei is the cause of melioidosis, a fatal tropical infectious disease, which has been reported to have a high rate of recurrence, even when an intensive dose of antibiotics is used.
26486518	5	12	theme	exopolysaccharide	752:768	arg1	production					770:779	exopolysaccharide production	752:779	exopolysaccharide production in biofilm matrix	752:797	The present study presents a correlation between exopolysaccharide production in biofilm matrix and antibiotic resistance in B. pseudomallei using bpsI, ppk, and rpoS mutant strains.
26486518	5	13	theme	present	707:713	arg1	study					715:719	The present study	703:719	The present study	703:719	The present study presents a correlation between exopolysaccharide production in biofilm matrix and antibiotic resistance in B. pseudomallei using bpsI, ppk, and rpoS mutant strains.
26486518	1	14	theme	antibiotics	347:357	arg1	dose					339:342	an intensive dose	326:342	an intensive dose of antibiotics	326:357	Burkholderia pseudomallei is the cause of melioidosis, a fatal tropical infectious disease, which has been reported to have a high rate of recurrence, even when an intensive dose of antibiotics is used.
26486518	3	15	from	penetration	575:585	arg1	pseudomallei					593:604	B. pseudomallei	590:604	B. pseudomallei	590:604	EPS in biofilms have been reported to be related to the limitation of antibiotic penetration in B. pseudomallei.
26486518	2	16	theme	Biofilm	368:374	arg1	formation					376:384	Biofilm formation	368:384	Biofilm formation	368:384	Biofilm formation is believed to be one of the possible causes of relapse because of its ability to increase drug resistance.
26486518	2	17	theme	possible	415:422	arg1	causes					424:429	the possible causes	411:429	the possible causes of relapse	411:440	Biofilm formation is believed to be one of the possible causes of relapse because of its ability to increase drug resistance.
26486518	7	18	theme	contents	1085:1092	arg1	ratios					1062:1067	Different ratios	1052:1067	Different ratios of carbohydrate contents in the exopolysaccharides of the mutants	1052:1133	Different ratios of carbohydrate contents in the exopolysaccharides of the mutants were detected, although they have the same components, including glucose, galactose, mannose, and rhamnose, with the exception being that no detectable rhamnose peak was observed in the bpsI mutant.
26486518	7	19	contain	have	1164:1167	arg2	mannose					1220:1226	mannose	1220:1226	mannose	1220:1226	Different ratios of carbohydrate contents in the exopolysaccharides of the mutants were detected, although they have the same components, including glucose, galactose, mannose, and rhamnose, with the exception being that no detectable rhamnose peak was observed in the bpsI mutant.
26486518	7	19	contain	have	1164:1167	arg2	rhamnose					1233:1240	rhamnose	1233:1240	rhamnose	1233:1240	Different ratios of carbohydrate contents in the exopolysaccharides of the mutants were detected, although they have the same components, including glucose, galactose, mannose, and rhamnose, with the exception being that no detectable rhamnose peak was observed in the bpsI mutant.
26486518	7	19	contain	have	1164:1167	arg2	glucose					1200:1206	glucose	1200:1206	glucose	1200:1206	Different ratios of carbohydrate contents in the exopolysaccharides of the mutants were detected, although they have the same components, including glucose, galactose, mannose, and rhamnose, with the exception being that no detectable rhamnose peak was observed in the bpsI mutant.
26486518	7	19	contain	have	1164:1167	arg2	components					1178:1187	the same components	1169:1187	the same components	1169:1187	Different ratios of carbohydrate contents in the exopolysaccharides of the mutants were detected, although they have the same components, including glucose, galactose, mannose, and rhamnose, with the exception being that no detectable rhamnose peak was observed in the bpsI mutant.
26486518	7	19	contain	have	1164:1167	arg1	they					1159:1162	they	1159:1162	they	1159:1162	Different ratios of carbohydrate contents in the exopolysaccharides of the mutants were detected, although they have the same components, including glucose, galactose, mannose, and rhamnose, with the exception being that no detectable rhamnose peak was observed in the bpsI mutant.
26486518	7	19	contain	have	1164:1167	arg2	galactose					1209:1217	galactose	1209:1217	galactose	1209:1217	Different ratios of carbohydrate contents in the exopolysaccharides of the mutants were detected, although they have the same components, including glucose, galactose, mannose, and rhamnose, with the exception being that no detectable rhamnose peak was observed in the bpsI mutant.
26486518	0	20	dep	Burkholderia	103:114	arg1	pseudomallei					116:127	pseudomallei	116:127	pseudomallei	116:127	Correlation between biofilm production, antibiotic susceptibility and exopolysaccharide composition in Burkholderia pseudomallei bpsI, ppk, and rpoS mutant strains.
26486518	6	21	dep	B.	983:984	arg1	pseudomallei					986:997	pseudomallei	986:997	pseudomallei	986:997	CLSM revealed a reduction in exopolysaccharide production and disabled micro-colony formation in B. pseudomallei mutants, which paralleled the antibiotic resistance.
26486518	7	22	theme	Different	1052:1060	arg1	ratios					1062:1067	Different ratios	1052:1067	Different ratios of carbohydrate contents in the exopolysaccharides of the mutants	1052:1133	Different ratios of carbohydrate contents in the exopolysaccharides of the mutants were detected, although they have the same components, including glucose, galactose, mannose, and rhamnose, with the exception being that no detectable rhamnose peak was observed in the bpsI mutant.
26486518	6	23	theme	micro-colony	957:968	arg1	formation					970:978	disabled micro-colony formation	948:978	disabled micro-colony formation	948:978	CLSM revealed a reduction in exopolysaccharide production and disabled micro-colony formation in B. pseudomallei mutants, which paralleled the antibiotic resistance.
26486518	6	24	theme	disabled	948:955	arg1	formation					970:978	disabled micro-colony formation	948:978	disabled micro-colony formation	948:978	CLSM revealed a reduction in exopolysaccharide production and disabled micro-colony formation in B. pseudomallei mutants, which paralleled the antibiotic resistance.
26486518	1	25	theme	melioidosis	207:217	arg1	cause					198:202	the cause	194:202	the cause of melioidosis, a fatal tropical infectious disease, which has been reported to have a high rate of recurrence, even when an intensive dose of antibiotics is used	194:365	Burkholderia pseudomallei is the cause of melioidosis, a fatal tropical infectious disease, which has been reported to have a high rate of recurrence, even when an intensive dose of antibiotics is used.
26486518	1	25	theme	melioidosis	207:217	arg1	pseudomallei					178:189	Burkholderia pseudomallei	165:189	Burkholderia pseudomallei	165:189	Burkholderia pseudomallei is the cause of melioidosis, a fatal tropical infectious disease, which has been reported to have a high rate of recurrence, even when an intensive dose of antibiotics is used.
26486518	0	26	theme	biofilm	20:26	arg1	production					28:37	biofilm production	20:37	biofilm production	20:37	Correlation between biofilm production, antibiotic susceptibility and exopolysaccharide composition in Burkholderia pseudomallei bpsI, ppk, and rpoS mutant strains.
26486518	7	27	from	ratios	1062:1067	arg1	exopolysaccharides					1101:1118	the exopolysaccharides	1097:1118	the exopolysaccharides of the mutants	1097:1133	Different ratios of carbohydrate contents in the exopolysaccharides of the mutants were detected, although they have the same components, including glucose, galactose, mannose, and rhamnose, with the exception being that no detectable rhamnose peak was observed in the bpsI mutant.
26486518	7	28	theme	carbohydrate	1072:1083	arg1	contents					1085:1092	carbohydrate contents	1072:1092	carbohydrate contents	1072:1092	Different ratios of carbohydrate contents in the exopolysaccharides of the mutants were detected, although they have the same components, including glucose, galactose, mannose, and rhamnose, with the exception being that no detectable rhamnose peak was observed in the bpsI mutant.
26486518	0	29	theme	antibiotic	40:49	arg1	susceptibility					51:64	antibiotic susceptibility	40:64	antibiotic susceptibility	40:64	Correlation between biofilm production, antibiotic susceptibility and exopolysaccharide composition in Burkholderia pseudomallei bpsI, ppk, and rpoS mutant strains.
26486518	1	30	theme	high	291:294	arg1	rate					296:299	a high rate	289:299	a high rate of recurrence	289:313	Burkholderia pseudomallei is the cause of melioidosis, a fatal tropical infectious disease, which has been reported to have a high rate of recurrence, even when an intensive dose of antibiotics is used.
26486518	0	31	theme	mutant	149:154	arg1	strains					156:162	rpoS mutant strains	144:162	rpoS mutant strains	144:162	Correlation between biofilm production, antibiotic susceptibility and exopolysaccharide composition in Burkholderia pseudomallei bpsI, ppk, and rpoS mutant strains.
26486518	3	32	from	limitation	550:559	arg1	pseudomallei					593:604	B. pseudomallei	590:604	B. pseudomallei	590:604	EPS in biofilms have been reported to be related to the limitation of antibiotic penetration in B. pseudomallei.
26486518	6	33	theme	antibiotic	1029:1038	arg1	resistance					1040:1049	the antibiotic resistance	1025:1049	the antibiotic resistance	1025:1049	CLSM revealed a reduction in exopolysaccharide production and disabled micro-colony formation in B. pseudomallei mutants, which paralleled the antibiotic resistance.
26486518	2	34	theme	relapse	434:440	arg1	causes					424:429	the possible causes	411:429	the possible causes of relapse	411:440	Biofilm formation is believed to be one of the possible causes of relapse because of its ability to increase drug resistance.
26486518	6	35	theme	exopolysaccharide	915:931	arg1	production					933:942	exopolysaccharide production	915:942	exopolysaccharide production	915:942	CLSM revealed a reduction in exopolysaccharide production and disabled micro-colony formation in B. pseudomallei mutants, which paralleled the antibiotic resistance.
26486518	0	36	theme	rpoS	144:147	arg1	strains					156:162	rpoS mutant strains	144:162	rpoS mutant strains	144:162	Correlation between biofilm production, antibiotic susceptibility and exopolysaccharide composition in Burkholderia pseudomallei bpsI, ppk, and rpoS mutant strains.
26486518	1	37	theme	fatal	222:226	arg1	disease					248:254	a fatal tropical infectious disease	220:254	a fatal tropical infectious disease	220:254	Burkholderia pseudomallei is the cause of melioidosis, a fatal tropical infectious disease, which has been reported to have a high rate of recurrence, even when an intensive dose of antibiotics is used.
26486518	1	37	theme	fatal	222:226	arg1	melioidosis					207:217	melioidosis	207:217	melioidosis	207:217	Burkholderia pseudomallei is the cause of melioidosis, a fatal tropical infectious disease, which has been reported to have a high rate of recurrence, even when an intensive dose of antibiotics is used.
26486518	8	38	theme	rpoS	1529:1532	arg1	genes					1534:1538	rpoS genes	1529:1538	rpoS genes	1529:1538	These results indicate that the correlation between these phenomena in the B. pseudomallei biofilm at least results from the exopolysaccharide, which may be under the regulation of bpsI, ppk, or rpoS genes.
26486518	6	39	from	reduction	902:910	arg1	formation					970:978	disabled micro-colony formation	948:978	disabled micro-colony formation	948:978	CLSM revealed a reduction in exopolysaccharide production and disabled micro-colony formation in B. pseudomallei mutants, which paralleled the antibiotic resistance.
26486518	6	39	from	reduction	902:910	arg1	mutants					999:1005	B. pseudomallei mutants	983:1005	B. pseudomallei mutants	983:1005	CLSM revealed a reduction in exopolysaccharide production and disabled micro-colony formation in B. pseudomallei mutants, which paralleled the antibiotic resistance.
26486518	6	39	from	reduction	902:910	arg1	production					933:942	exopolysaccharide production	915:942	exopolysaccharide production	915:942	CLSM revealed a reduction in exopolysaccharide production and disabled micro-colony formation in B. pseudomallei mutants, which paralleled the antibiotic resistance.
26486518	8	40	theme	bpsI	1515:1518	arg1	regulation					1501:1510	the regulation	1497:1510	the regulation of bpsI, ppk, or rpoS genes	1497:1538	These results indicate that the correlation between these phenomena in the B. pseudomallei biofilm at least results from the exopolysaccharide, which may be under the regulation of bpsI, ppk, or rpoS genes.
26486518	2	41	theme	causes	424:429	arg1	one					404:406	one	404:406	one	404:406	Biofilm formation is believed to be one of the possible causes of relapse because of its ability to increase drug resistance.
26486518	2	41	theme	causes	424:429	arg1	causes					424:429	the possible causes	411:429	the possible causes of relapse	411:440	Biofilm formation is believed to be one of the possible causes of relapse because of its ability to increase drug resistance.
26486518	1	42	theme	recurrence	304:313	arg1	rate					296:299	a high rate	289:299	a high rate of recurrence	289:313	Burkholderia pseudomallei is the cause of melioidosis, a fatal tropical infectious disease, which has been reported to have a high rate of recurrence, even when an intensive dose of antibiotics is used.
26486518	5	43	from	resistance	814:823	arg1	matrix					792:797	biofilm matrix	784:797	biofilm matrix	784:797	The present study presents a correlation between exopolysaccharide production in biofilm matrix and antibiotic resistance in B. pseudomallei using bpsI, ppk, and rpoS mutant strains.
26486518	5	43	from	resistance	814:823	arg1	pseudomallei					831:842	B. pseudomallei	828:842	B. pseudomallei	828:842	The present study presents a correlation between exopolysaccharide production in biofilm matrix and antibiotic resistance in B. pseudomallei using bpsI, ppk, and rpoS mutant strains.
26486518	8	44	dep	B.	1409:1410	arg1	pseudomallei					1412:1423	pseudomallei	1412:1423	pseudomallei	1412:1423	These results indicate that the correlation between these phenomena in the B. pseudomallei biofilm at least results from the exopolysaccharide, which may be under the regulation of bpsI, ppk, or rpoS genes.
26486518	8	45	theme	B.	1409:1410	arg1	biofilm					1425:1431	the B. pseudomallei biofilm	1405:1431	the B. pseudomallei biofilm at least	1405:1440	These results indicate that the correlation between these phenomena in the B. pseudomallei biofilm at least results from the exopolysaccharide, which may be under the regulation of bpsI, ppk, or rpoS genes.
26486518	8	46	theme	ppk	1521:1523	arg1	regulation					1501:1510	the regulation	1497:1510	the regulation of bpsI, ppk, or rpoS genes	1497:1538	These results indicate that the correlation between these phenomena in the B. pseudomallei biofilm at least results from the exopolysaccharide, which may be under the regulation of bpsI, ppk, or rpoS genes.
26486518	3	47	theme	penetration	575:585	arg1	limitation					550:559	the limitation	546:559	the limitation of antibiotic penetration in B. pseudomallei	546:604	EPS in biofilms have been reported to be related to the limitation of antibiotic penetration in B. pseudomallei.
26486518	1	48	theme	tropical	228:235	arg1	disease					248:254	a fatal tropical infectious disease	220:254	a fatal tropical infectious disease	220:254	Burkholderia pseudomallei is the cause of melioidosis, a fatal tropical infectious disease, which has been reported to have a high rate of recurrence, even when an intensive dose of antibiotics is used.
26486518	1	48	theme	tropical	228:235	arg1	melioidosis					207:217	melioidosis	207:217	melioidosis	207:217	Burkholderia pseudomallei is the cause of melioidosis, a fatal tropical infectious disease, which has been reported to have a high rate of recurrence, even when an intensive dose of antibiotics is used.
26486518	7	49	located	observed	1305:1312	arg2	peak					1296:1299	no detectable rhamnose peak	1273:1299	no detectable rhamnose peak	1273:1299	Different ratios of carbohydrate contents in the exopolysaccharides of the mutants were detected, although they have the same components, including glucose, galactose, mannose, and rhamnose, with the exception being that no detectable rhamnose peak was observed in the bpsI mutant.
26486518	7	49	located	observed	1305:1312	arg1	mutant					1326:1331	the bpsI mutant	1317:1331	the bpsI mutant	1317:1331	Different ratios of carbohydrate contents in the exopolysaccharides of the mutants were detected, although they have the same components, including glucose, galactose, mannose, and rhamnose, with the exception being that no detectable rhamnose peak was observed in the bpsI mutant.
26486518	5	50	theme	antibiotic	803:812	arg1	resistance					814:823	antibiotic resistance	803:823	antibiotic resistance in B. pseudomallei	803:842	The present study presents a correlation between exopolysaccharide production in biofilm matrix and antibiotic resistance in B. pseudomallei using bpsI, ppk, and rpoS mutant strains.
26486518	4	51	theme	antibiotics	675:685	arg1	diffusion					662:670	the diffusion	658:670	the diffusion of antibiotics	658:685	However, the mechanisms by which biofilms restrict the diffusion of antibiotics remain unclear.
26486518	1	52	theme	infectious	237:246	arg1	disease					248:254	a fatal tropical infectious disease	220:254	a fatal tropical infectious disease	220:254	Burkholderia pseudomallei is the cause of melioidosis, a fatal tropical infectious disease, which has been reported to have a high rate of recurrence, even when an intensive dose of antibiotics is used.
26486518	1	52	theme	infectious	237:246	arg1	melioidosis					207:217	melioidosis	207:217	melioidosis	207:217	Burkholderia pseudomallei is the cause of melioidosis, a fatal tropical infectious disease, which has been reported to have a high rate of recurrence, even when an intensive dose of antibiotics is used.
26486518	7	53	theme	detectable	1276:1285	arg1	peak					1296:1299	no detectable rhamnose peak	1273:1299	no detectable rhamnose peak	1273:1299	Different ratios of carbohydrate contents in the exopolysaccharides of the mutants were detected, although they have the same components, including glucose, galactose, mannose, and rhamnose, with the exception being that no detectable rhamnose peak was observed in the bpsI mutant.
26486518	7	54	theme	bpsI	1321:1324	arg1	mutant					1326:1331	the bpsI mutant	1317:1331	the bpsI mutant	1317:1331	Different ratios of carbohydrate contents in the exopolysaccharides of the mutants were detected, although they have the same components, including glucose, galactose, mannose, and rhamnose, with the exception being that no detectable rhamnose peak was observed in the bpsI mutant.
26486518	7	55	theme	rhamnose	1287:1294	arg1	peak					1296:1299	no detectable rhamnose peak	1273:1299	no detectable rhamnose peak	1273:1299	Different ratios of carbohydrate contents in the exopolysaccharides of the mutants were detected, although they have the same components, including glucose, galactose, mannose, and rhamnose, with the exception being that no detectable rhamnose peak was observed in the bpsI mutant.
26486518	7	56	theme	same	1173:1176	arg1	glucose					1200:1206	glucose	1200:1206	glucose	1200:1206	Different ratios of carbohydrate contents in the exopolysaccharides of the mutants were detected, although they have the same components, including glucose, galactose, mannose, and rhamnose, with the exception being that no detectable rhamnose peak was observed in the bpsI mutant.
26486518	7	56	theme	same	1173:1176	arg1	mannose					1220:1226	mannose	1220:1226	mannose	1220:1226	Different ratios of carbohydrate contents in the exopolysaccharides of the mutants were detected, although they have the same components, including glucose, galactose, mannose, and rhamnose, with the exception being that no detectable rhamnose peak was observed in the bpsI mutant.
26486518	7	56	theme	same	1173:1176	arg1	rhamnose					1233:1240	rhamnose	1233:1240	rhamnose	1233:1240	Different ratios of carbohydrate contents in the exopolysaccharides of the mutants were detected, although they have the same components, including glucose, galactose, mannose, and rhamnose, with the exception being that no detectable rhamnose peak was observed in the bpsI mutant.
26486518	7	56	theme	same	1173:1176	arg1	galactose					1209:1217	galactose	1209:1217	galactose	1209:1217	Different ratios of carbohydrate contents in the exopolysaccharides of the mutants were detected, although they have the same components, including glucose, galactose, mannose, and rhamnose, with the exception being that no detectable rhamnose peak was observed in the bpsI mutant.
26486518	7	56	theme	same	1173:1176	arg1	components					1178:1187	the same components	1169:1187	the same components	1169:1187	Different ratios of carbohydrate contents in the exopolysaccharides of the mutants were detected, although they have the same components, including glucose, galactose, mannose, and rhamnose, with the exception being that no detectable rhamnose peak was observed in the bpsI mutant.
26486518	8	57	theme	genes	1534:1538	arg1	regulation					1501:1510	the regulation	1497:1510	the regulation of bpsI, ppk, or rpoS genes	1497:1538	These results indicate that the correlation between these phenomena in the B. pseudomallei biofilm at least results from the exopolysaccharide, which may be under the regulation of bpsI, ppk, or rpoS genes.
26486518	0	58	theme	exopolysaccharide	70:86	arg1	composition					88:98	exopolysaccharide composition	70:98	exopolysaccharide composition	70:98	Correlation between biofilm production, antibiotic susceptibility and exopolysaccharide composition in Burkholderia pseudomallei bpsI, ppk, and rpoS mutant strains.
26486518	5	59	from	production	770:779	arg1	matrix					792:797	biofilm matrix	784:797	biofilm matrix	784:797	The present study presents a correlation between exopolysaccharide production in biofilm matrix and antibiotic resistance in B. pseudomallei using bpsI, ppk, and rpoS mutant strains.
26486518	5	59	from	production	770:779	arg1	pseudomallei					831:842	B. pseudomallei	828:842	B. pseudomallei	828:842	The present study presents a correlation between exopolysaccharide production in biofilm matrix and antibiotic resistance in B. pseudomallei using bpsI, ppk, and rpoS mutant strains.
26486518	3	60	theme	antibiotic	564:573	arg1	penetration					575:585	antibiotic penetration	564:585	antibiotic penetration in B. pseudomallei	564:604	EPS in biofilms have been reported to be related to the limitation of antibiotic penetration in B. pseudomallei.
26486518	0	61	from	Correlation	0:10	arg1	bpsI					129:132	Burkholderia pseudomallei bpsI	103:132	Burkholderia pseudomallei bpsI	103:132	Correlation between biofilm production, antibiotic susceptibility and exopolysaccharide composition in Burkholderia pseudomallei bpsI, ppk, and rpoS mutant strains.
26486518	0	61	from	Correlation	0:10	arg1	strains					156:162	rpoS mutant strains	144:162	rpoS mutant strains	144:162	Correlation between biofilm production, antibiotic susceptibility and exopolysaccharide composition in Burkholderia pseudomallei bpsI, ppk, and rpoS mutant strains.
26486518	0	61	from	Correlation	0:10	arg1	ppk					135:137	ppk	135:137	ppk	135:137	Correlation between biofilm production, antibiotic susceptibility and exopolysaccharide composition in Burkholderia pseudomallei bpsI, ppk, and rpoS mutant strains.
26486518	5	62	theme	biofilm	784:790	arg1	matrix					792:797	biofilm matrix	784:797	biofilm matrix	784:797	The present study presents a correlation between exopolysaccharide production in biofilm matrix and antibiotic resistance in B. pseudomallei using bpsI, ppk, and rpoS mutant strains.
26486518	3	63	from	EPS	494:496	arg1	biofilms					501:508	biofilms	501:508	biofilms	501:508	EPS in biofilms have been reported to be related to the limitation of antibiotic penetration in B. pseudomallei.
28390831	3	0	theme	A.	255:256	arg1	gum					267:269	A. nilotica gum	255:269	A. nilotica gum	255:269	A. nilotica gum was collected from Maharashtra, India and characterised for its proximate analysis, physicochemical, functional, rheological and thermal properties.
28390831	8	1	theme	viscosity	960:968	arg1	profile					970:976	viscosity profile	960:976	viscosity profile	960:976	Investigations of the flow curves of both gums revealed the diminutive difference in viscosity profile.
28390831	6	2	theme	typical	642:648	arg1	trend					650:654	the typical trend	638:654	the typical trend of polysaccharides for both the gums	638:691	FTIR spectrums revealed the typical trend of polysaccharides for both the gums, however, the difference was observed in fingerprint region.
28390831	10	3	theme	good	1191:1194	arg1	resemblance					1196:1206	good resemblance	1191:1206	good resemblance with AG	1191:1214	However, ANG shows good resemblance with AG and can be substituted for numerous applications in food and pharmaceutical industry.
28390831	9	4	theme	concentration	983:995	arg1	difference					997:1006	The concentration difference	979:1006	The concentration difference in the monosaccharides of polysaccharides and proximate analysis of gums	979:1079	The concentration difference in the monosaccharides of polysaccharides and proximate analysis of gums could be the responsible for the difference in rheological and thermal properties of gums.
28390831	9	4	theme	concentration	983:995	arg1	responsible					1094:1104	responsible	1094:1104	responsible	1094:1104	The concentration difference in the monosaccharides of polysaccharides and proximate analysis of gums could be the responsible for the difference in rheological and thermal properties of gums.
28390831	3	5	theme	nilotica	258:265	arg1	gum					267:269	A. nilotica gum	255:269	A. nilotica gum	255:269	A. nilotica gum was collected from Maharashtra, India and characterised for its proximate analysis, physicochemical, functional, rheological and thermal properties.
28390831	5	6	theme	gums	533:536	arg1	composition					514:524	The sugar composition	504:524	The sugar composition of the gums	504:536	The sugar composition of the gums indicated the presence of arabinose, galactose, and rhamnose in ANG and AG.
28390831	6	7	located	observed	722:729	arg1	region					746:751	fingerprint region	734:751	fingerprint region	734:751	FTIR spectrums revealed the typical trend of polysaccharides for both the gums, however, the difference was observed in fingerprint region.
28390831	6	7	located	observed	722:729	arg2	difference					707:716	the difference	703:716	the difference	703:716	FTIR spectrums revealed the typical trend of polysaccharides for both the gums, however, the difference was observed in fingerprint region.
28390831	1	8	located	found	102:106	arg2	species					74:80	900 species	70:80	900 species of Acacia trees	70:96	Over 900 species of Acacia trees are found on earth, most of them produce gums.
28390831	1	8	located	found	102:106	arg1	earth					111:115	earth	111:115	earth	111:115	Over 900 species of Acacia trees are found on earth, most of them produce gums.
28390831	5	9	theme	rhamnose	590:597	arg1	presence					552:559	the presence	548:559	the presence of arabinose, galactose, and rhamnose in ANG and AG	548:611	The sugar composition of the gums indicated the presence of arabinose, galactose, and rhamnose in ANG and AG.
28390831	8	10	theme	curves	902:907	arg1	Investigations					875:888	Investigations	875:888	Investigations of the flow curves of both gums	875:920	Investigations of the flow curves of both gums revealed the diminutive difference in viscosity profile.
28390831	9	11	from	difference	997:1006	arg1	monosaccharides					1015:1029	the monosaccharides	1011:1029	the monosaccharides of polysaccharides and proximate analysis of gums	1011:1079	The concentration difference in the monosaccharides of polysaccharides and proximate analysis of gums could be the responsible for the difference in rheological and thermal properties of gums.
28390831	7	12	theme	flow	797:800	arg1	measurements					808:819	flow curve measurements	797:819	flow curve measurements of gums	797:827	The rheological outcomes were derived from flow curve measurements of gums at different concentrations and temperatures.
28390831	8	13	theme	gums	917:920	arg1	curves					902:907	the flow curves	893:907	the flow curves of both gums	893:920	Investigations of the flow curves of both gums revealed the diminutive difference in viscosity profile.
28390831	10	14	theme	numerous	1243:1250	arg1	applications					1252:1263	numerous applications	1243:1263	numerous applications in food and pharmaceutical industry	1243:1299	However, ANG shows good resemblance with AG and can be substituted for numerous applications in food and pharmaceutical industry.
28390831	9	15	theme	polysaccharides	1034:1048	arg1	monosaccharides					1015:1029	the monosaccharides	1011:1029	the monosaccharides of polysaccharides and proximate analysis of gums	1011:1079	The concentration difference in the monosaccharides of polysaccharides and proximate analysis of gums could be the responsible for the difference in rheological and thermal properties of gums.
28390831	9	16	theme	thermal	1144:1150	arg1	properties					1152:1161	rheological and thermal properties	1128:1161	properties	1152:1161	The concentration difference in the monosaccharides of polysaccharides and proximate analysis of gums could be the responsible for the difference in rheological and thermal properties of gums.
28390831	2	17	theme	species	214:220	arg1	one					177:179	one	177:179	one	177:179	Acacia nilotica (Babul tree) is one of the major gum-yielding acacia species found in he Indian subcontinent.
28390831	2	17	theme	species	214:220	arg1	species					214:220	the major gum-yielding acacia species	184:220	the major gum-yielding acacia species found in he Indian subcontinent	184:252	Acacia nilotica (Babul tree) is one of the major gum-yielding acacia species found in he Indian subcontinent.
28390831	3	18	theme	functional	372:381	arg1	analysis					345:352	its proximate analysis	331:352	its proximate analysis	331:352	A. nilotica gum was collected from Maharashtra, India and characterised for its proximate analysis, physicochemical, functional, rheological and thermal properties.
28390831	3	18	theme	functional	372:381	arg1	properties					408:417	physicochemical, functional, rheological and thermal properties	355:417	physicochemical, functional, rheological and thermal properties	355:417	A. nilotica gum was collected from Maharashtra, India and characterised for its proximate analysis, physicochemical, functional, rheological and thermal properties.
28390831	5	19	theme	arabinose	564:572	arg1	presence					552:559	the presence	548:559	the presence of arabinose, galactose, and rhamnose in ANG and AG	548:611	The sugar composition of the gums indicated the presence of arabinose, galactose, and rhamnose in ANG and AG.
28390831	5	20	theme	sugar	508:512	arg1	composition					514:524	The sugar composition	504:524	The sugar composition of the gums	504:536	The sugar composition of the gums indicated the presence of arabinose, galactose, and rhamnose in ANG and AG.
28390831	2	21	theme	acacia	207:212	arg1	species					214:220	the major gum-yielding acacia species	184:220	the major gum-yielding acacia species found in he Indian subcontinent	184:252	Acacia nilotica (Babul tree) is one of the major gum-yielding acacia species found in he Indian subcontinent.
28390831	7	22	theme	rheological	758:768	arg1	outcomes					770:777	The rheological outcomes	754:777	The rheological outcomes	754:777	The rheological outcomes were derived from flow curve measurements of gums at different concentrations and temperatures.
28390831	9	23	from	difference	1114:1123	arg1	properties					1152:1161	rheological and thermal properties	1128:1161	properties	1152:1161	The concentration difference in the monosaccharides of polysaccharides and proximate analysis of gums could be the responsible for the difference in rheological and thermal properties of gums.
28390831	6	24	theme	FTIR	614:617	arg1	spectrums					619:627	FTIR spectrums	614:627	FTIR spectrums	614:627	FTIR spectrums revealed the typical trend of polysaccharides for both the gums, however, the difference was observed in fingerprint region.
28390831	3	25	theme	physicochemical	355:369	arg1	analysis					345:352	its proximate analysis	331:352	its proximate analysis	331:352	A. nilotica gum was collected from Maharashtra, India and characterised for its proximate analysis, physicochemical, functional, rheological and thermal properties.
28390831	3	25	theme	physicochemical	355:369	arg1	properties					408:417	physicochemical, functional, rheological and thermal properties	355:417	physicochemical, functional, rheological and thermal properties	355:417	A. nilotica gum was collected from Maharashtra, India and characterised for its proximate analysis, physicochemical, functional, rheological and thermal properties.
28390831	0	26	theme	Comparative	0:10	arg1	study					12:16	Comparative study	0:16	Comparative study of Acacia	0:26	Comparative study of Acacia nilotica exudate gum and acacia gum.
28390831	5	27	theme	galactose	575:583	arg1	presence					552:559	the presence	548:559	the presence of arabinose, galactose, and rhamnose in ANG and AG	548:611	The sugar composition of the gums indicated the presence of arabinose, galactose, and rhamnose in ANG and AG.
28390831	9	28	theme	gums	1166:1169	arg1	properties					1152:1161	rheological and thermal properties	1128:1161	properties	1152:1161	The concentration difference in the monosaccharides of polysaccharides and proximate analysis of gums could be the responsible for the difference in rheological and thermal properties of gums.
28390831	0	29	theme	Acacia	21:26	arg1	study					12:16	Comparative study	0:16	Comparative study of Acacia	0:26	Comparative study of Acacia nilotica exudate gum and acacia gum.
28390831	8	30	theme	diminutive	935:944	arg1	difference					946:955	the diminutive difference	931:955	the diminutive difference in viscosity profile	931:976	Investigations of the flow curves of both gums revealed the diminutive difference in viscosity profile.
28390831	9	31	theme	proximate	1054:1062	arg1	analysis					1064:1071	proximate analysis	1054:1071	proximate analysis of gums	1054:1079	The concentration difference in the monosaccharides of polysaccharides and proximate analysis of gums could be the responsible for the difference in rheological and thermal properties of gums.
28390831	3	32	theme	thermal	400:406	arg1	analysis					345:352	its proximate analysis	331:352	its proximate analysis	331:352	A. nilotica gum was collected from Maharashtra, India and characterised for its proximate analysis, physicochemical, functional, rheological and thermal properties.
28390831	3	32	theme	thermal	400:406	arg1	properties					408:417	physicochemical, functional, rheological and thermal properties	355:417	physicochemical, functional, rheological and thermal properties	355:417	A. nilotica gum was collected from Maharashtra, India and characterised for its proximate analysis, physicochemical, functional, rheological and thermal properties.
28390831	1	33	theme	Acacia	85:90	arg1	trees					92:96	Acacia trees	85:96	Acacia trees	85:96	Over 900 species of Acacia trees are found on earth, most of them produce gums.
28390831	9	34	theme	analysis	1064:1071	arg1	monosaccharides					1015:1029	the monosaccharides	1011:1029	the monosaccharides of polysaccharides and proximate analysis of gums	1011:1079	The concentration difference in the monosaccharides of polysaccharides and proximate analysis of gums could be the responsible for the difference in rheological and thermal properties of gums.
28390831	0	35	theme	exudate	37:43	arg1	gum					45:47	exudate gum	37:47	exudate gum	37:47	Comparative study of Acacia nilotica exudate gum and acacia gum.
28390831	3	36	theme	rheological	384:394	arg1	analysis					345:352	its proximate analysis	331:352	its proximate analysis	331:352	A. nilotica gum was collected from Maharashtra, India and characterised for its proximate analysis, physicochemical, functional, rheological and thermal properties.
28390831	3	36	theme	rheological	384:394	arg1	properties					408:417	physicochemical, functional, rheological and thermal properties	355:417	physicochemical, functional, rheological and thermal properties	355:417	A. nilotica gum was collected from Maharashtra, India and characterised for its proximate analysis, physicochemical, functional, rheological and thermal properties.
28390831	2	37	located	found	222:226	arg1	Indian					234:239	Indian	234:239	Indian	234:239	Acacia nilotica (Babul tree) is one of the major gum-yielding acacia species found in he Indian subcontinent.
28390831	2	37	located	found	222:226	arg2	species					214:220	the major gum-yielding acacia species	184:220	the major gum-yielding acacia species found in he Indian subcontinent	184:252	Acacia nilotica (Babul tree) is one of the major gum-yielding acacia species found in he Indian subcontinent.
28390831	5	38	attach	presence	552:559	arg1	AG					610:611	AG	610:611	AG	610:611	The sugar composition of the gums indicated the presence of arabinose, galactose, and rhamnose in ANG and AG.
28390831	5	38	attach	presence	552:559	arg1	ANG					602:604	ANG	602:604	ANG	602:604	The sugar composition of the gums indicated the presence of arabinose, galactose, and rhamnose in ANG and AG.
28390831	5	38	attach	presence	552:559	arg2	rhamnose					590:597	rhamnose	590:597	rhamnose	590:597	The sugar composition of the gums indicated the presence of arabinose, galactose, and rhamnose in ANG and AG.
28390831	5	38	attach	presence	552:559	arg2	galactose					575:583	galactose	575:583	galactose	575:583	The sugar composition of the gums indicated the presence of arabinose, galactose, and rhamnose in ANG and AG.
28390831	5	38	attach	presence	552:559	arg2	arabinose					564:572	arabinose	564:572	arabinose	564:572	The sugar composition of the gums indicated the presence of arabinose, galactose, and rhamnose in ANG and AG.
28390831	9	39	theme	gums	1076:1079	arg1	polysaccharides					1034:1048	polysaccharides	1034:1048	polysaccharides	1034:1048	The concentration difference in the monosaccharides of polysaccharides and proximate analysis of gums could be the responsible for the difference in rheological and thermal properties of gums.
28390831	9	39	theme	gums	1076:1079	arg1	analysis					1064:1071	proximate analysis	1054:1071	proximate analysis of gums	1054:1079	The concentration difference in the monosaccharides of polysaccharides and proximate analysis of gums could be the responsible for the difference in rheological and thermal properties of gums.
28390831	4	40	theme	Acacia	487:492	arg1	gum					494:496	commercially available Acacia gum	464:496	commercially available Acacia gum (AG)	464:501	These properties further were compared with commercially available Acacia gum (AG).
28390831	4	40	theme	Acacia	487:492	arg1	AG					499:500	AG	499:500	AG	499:500	These properties further were compared with commercially available Acacia gum (AG).
28390831	7	41	attach	derived	784:790	arg2	outcomes					770:777	The rheological outcomes	754:777	The rheological outcomes	754:777	The rheological outcomes were derived from flow curve measurements of gums at different concentrations and temperatures.
28390831	7	41	attach	derived	784:790	arg1	measurements					808:819	flow curve measurements	797:819	flow curve measurements of gums	797:827	The rheological outcomes were derived from flow curve measurements of gums at different concentrations and temperatures.
28390831	10	42	with	resemblance	1196:1206	arg1	AG					1213:1214	AG	1213:1214	AG	1213:1214	However, ANG shows good resemblance with AG and can be substituted for numerous applications in food and pharmaceutical industry.
28390831	10	43	theme	pharmaceutical	1277:1290	arg1	industry					1292:1299	pharmaceutical industry	1277:1299	pharmaceutical industry	1277:1299	However, ANG shows good resemblance with AG and can be substituted for numerous applications in food and pharmaceutical industry.
28390831	1	44	theme	trees	92:96	arg1	species					74:80	900 species	70:80	900 species of Acacia trees	70:96	Over 900 species of Acacia trees are found on earth, most of them produce gums.
28390831	3	45	theme	proximate	335:343	arg1	analysis					345:352	its proximate analysis	331:352	its proximate analysis	331:352	A. nilotica gum was collected from Maharashtra, India and characterised for its proximate analysis, physicochemical, functional, rheological and thermal properties.
28390831	3	45	theme	proximate	335:343	arg1	properties					408:417	physicochemical, functional, rheological and thermal properties	355:417	physicochemical, functional, rheological and thermal properties	355:417	A. nilotica gum was collected from Maharashtra, India and characterised for its proximate analysis, physicochemical, functional, rheological and thermal properties.
28390831	2	46	theme	gum-yielding	194:205	arg1	species					214:220	the major gum-yielding acacia species	184:220	the major gum-yielding acacia species found in he Indian subcontinent	184:252	Acacia nilotica (Babul tree) is one of the major gum-yielding acacia species found in he Indian subcontinent.
28390831	9	47	theme	rheological	1128:1138	arg1	properties					1152:1161	rheological and thermal properties	1128:1161	properties	1152:1161	The concentration difference in the monosaccharides of polysaccharides and proximate analysis of gums could be the responsible for the difference in rheological and thermal properties of gums.
28390831	7	48	theme	different	832:840	arg1	concentrations					842:855	different concentrations	832:855	different concentrations	832:855	The rheological outcomes were derived from flow curve measurements of gums at different concentrations and temperatures.
28390831	2	49	theme	major	188:192	arg1	species					214:220	the major gum-yielding acacia species	184:220	the major gum-yielding acacia species found in he Indian subcontinent	184:252	Acacia nilotica (Babul tree) is one of the major gum-yielding acacia species found in he Indian subcontinent.
28390831	6	50	theme	polysaccharides	659:673	arg1	trend					650:654	the typical trend	638:654	the typical trend of polysaccharides for both the gums	638:691	FTIR spectrums revealed the typical trend of polysaccharides for both the gums, however, the difference was observed in fingerprint region.
28390831	2	51	theme	Acacia	145:150	arg1	nilotica					152:159	Acacia nilotica	145:159	Acacia nilotica (Babul tree)	145:172	Acacia nilotica (Babul tree) is one of the major gum-yielding acacia species found in he Indian subcontinent.
28390831	2	51	theme	Acacia	145:150	arg1	Babul					162:166	Babul	162:166	Babul	162:166	Acacia nilotica (Babul tree) is one of the major gum-yielding acacia species found in he Indian subcontinent.
28390831	8	52	from	difference	946:955	arg1	profile					970:976	viscosity profile	960:976	viscosity profile	960:976	Investigations of the flow curves of both gums revealed the diminutive difference in viscosity profile.
28390831	7	53	theme	gums	824:827	arg1	measurements					808:819	flow curve measurements	797:819	flow curve measurements of gums	797:827	The rheological outcomes were derived from flow curve measurements of gums at different concentrations and temperatures.
28390831	6	54	theme	fingerprint	734:744	arg1	region					746:751	fingerprint region	734:751	fingerprint region	734:751	FTIR spectrums revealed the typical trend of polysaccharides for both the gums, however, the difference was observed in fingerprint region.
28390831	0	55	theme	acacia	53:58	arg1	gum					60:62	acacia gum	53:62	acacia gum	53:62	Comparative study of Acacia nilotica exudate gum and acacia gum.
28390831	10	56	from	applications	1252:1263	arg1	food					1268:1271	food	1268:1271	food	1268:1271	However, ANG shows good resemblance with AG and can be substituted for numerous applications in food and pharmaceutical industry.
28390831	10	56	from	applications	1252:1263	arg1	industry					1292:1299	pharmaceutical industry	1277:1299	pharmaceutical industry	1277:1299	However, ANG shows good resemblance with AG and can be substituted for numerous applications in food and pharmaceutical industry.
28390831	8	57	theme	flow	897:900	arg1	curves					902:907	the flow curves	893:907	the flow curves of both gums	893:920	Investigations of the flow curves of both gums revealed the diminutive difference in viscosity profile.
28390831	5	58	from	presence	552:559	arg1	AG					610:611	AG	610:611	AG	610:611	The sugar composition of the gums indicated the presence of arabinose, galactose, and rhamnose in ANG and AG.
28390831	5	58	from	presence	552:559	arg1	ANG					602:604	ANG	602:604	ANG	602:604	The sugar composition of the gums indicated the presence of arabinose, galactose, and rhamnose in ANG and AG.
28390831	7	59	theme	curve	802:806	arg1	measurements					808:819	flow curve measurements	797:819	flow curve measurements of gums	797:827	The rheological outcomes were derived from flow curve measurements of gums at different concentrations and temperatures.
28390831	4	60	theme	available	477:485	arg1	gum					494:496	commercially available Acacia gum	464:496	commercially available Acacia gum (AG)	464:501	These properties further were compared with commercially available Acacia gum (AG).
28390831	4	60	theme	available	477:485	arg1	AG					499:500	AG	499:500	AG	499:500	These properties further were compared with commercially available Acacia gum (AG).
27405739	15	0	theme	high	1797:1800	arg1	capacity					1820:1827	high ferrous chelating capacity	1797:1827	high ferrous chelating capacity	1797:1827	AEPS showed high activity as reducing agent, high ferrous chelating capacity and caused a significant decrease in a concentration-dependent manner of hydroxyl radical.
27405739	15	0	theme	high	1797:1800	arg1	agent					1790:1794	reducing agent	1781:1794	reducing agent	1781:1794	AEPS showed high activity as reducing agent, high ferrous chelating capacity and caused a significant decrease in a concentration-dependent manner of hydroxyl radical.
27405739	9	1	theme	scavenging	1226:1235	arg1	activity					1237:1244	hydroxyl and superoxide radical scavenging activity	1194:1244	hydroxyl and superoxide radical scavenging activity	1194:1244	AEPS was analyzed for its antioxidant propriety by means of total antioxidant capacity, DPPH radicals scavenging assay, ferrous chelating ability and hydroxyl and superoxide radical scavenging activity.
27405739	3	2	theme	high	497:500	arg1	bio-products					521:532	high value thermostable bio-products	497:532	high value thermostable bio-products	497:532	The ability of microalgae to sustain at the high temperature makes them potential producers of high value thermostable bio-products.
27405739	22	3	with	relationships	2562:2574	arg1	function					2585:2592	its function	2581:2592	its function	2581:2592	Further work will identify and purify the active polysaccharides to enhance our understanding of their complete structure and relationships with its function.
27405739	7	4	theme	monosaccharide	872:885	arg1	composition					887:897	monosaccharide composition	872:897	monosaccharide composition	872:897	The dry weight, volatile dry weight, elemental analysis, monosaccharide composition and IR-spectroscopy analysis.
27405739	10	5	theme	antiproliferative	1251:1267	arg1	activity					1269:1276	The antiproliferative activity	1247:1276	The antiproliferative activity of AEPS	1247:1284	The antiproliferative activity of AEPS was evaluated for HepG2 and Caco-2 cells using the MTT assay.
27405739	8	6	theme	colorimetric	1024:1035	arg1	assay					1037:1041	colorimetric assay	1024:1041	colorimetric assay	1024:1041	Carbohydrate, uronic acid, ester sulfate and protein concentrations were also determined using colorimetric assay.
27405739	4	7	theme	thermophilic	657:668	arg1	sp					692:693	the Tunisian thermophilic microalgae Graesiella sp	644:693	the Tunisian thermophilic microalgae Graesiella sp	644:693	This study aimed to explore the therapeutic potential of the aqueous extracellular polysaccharides (AEPS) of the Tunisian thermophilic microalgae Graesiella sp.
27405739	1	8	theme	Tunisian	202:209	arg1	water					222:226	Tunisian geothermal water	202:226	Tunisian geothermal water	202:226	BACKGROUND For thousands of years, Tunisian geothermal water has been used in bathing.
27405739	4	9	theme	Graesiella	681:690	arg1	sp					692:693	the Tunisian thermophilic microalgae Graesiella sp	644:693	the Tunisian thermophilic microalgae Graesiella sp	644:693	This study aimed to explore the therapeutic potential of the aqueous extracellular polysaccharides (AEPS) of the Tunisian thermophilic microalgae Graesiella sp.
27405739	14	10	dep	groups	1744:1749	arg1	sulfate					1736:1742	sulfate	1736:1742	sulfate	1736:1742	The FT-IR revealed the presence of carboxyl, hydroxyl, amine and sulfate groups.
27405739	14	10	dep	groups	1744:1749	arg1	amine					1726:1730	amine	1726:1730	amine	1726:1730	The FT-IR revealed the presence of carboxyl, hydroxyl, amine and sulfate groups.
27405739	14	10	dep	groups	1744:1749	arg1	groups					1744:1749	carboxyl, hydroxyl, amine and sulfate groups	1706:1749	groups	1744:1749	The FT-IR revealed the presence of carboxyl, hydroxyl, amine and sulfate groups.
27405739	14	10	dep	groups	1744:1749	arg1	hydroxyl					1716:1723	carboxyl, hydroxyl, amine and sulfate groups	1706:1749	hydroxyl	1716:1723	The FT-IR revealed the presence of carboxyl, hydroxyl, amine and sulfate groups.
27405739	0	11	theme	Graesiella	152:161	arg1	sp					163:164	the thermophilic microalgae Graesiella sp	124:164	the thermophilic microalgae Graesiella sp	124:164	Partial characterization and antioxidant and antiproliferative activities of the aqueous extracellular polysaccharides from the thermophilic microalgae Graesiella sp.
27405739	20	12	theme	antioxidant	2308:2318	arg1	activities					2342:2351	antioxidant and antiproliferative activities	2308:2351	antioxidant and antiproliferative activities	2308:2351	AEPS exhibits antioxidant and antiproliferative activities.
27405739	7	13	theme	dry	840:842	arg1	weight					844:849	volatile dry weight	831:849	volatile dry weight	831:849	The dry weight, volatile dry weight, elemental analysis, monosaccharide composition and IR-spectroscopy analysis.
27405739	17	14	dep	0.01	2046:2049	arg1	to					2051:2052	to	2051:2052	to	2051:2052	AEPS treatment (from 0.01 to 2.5 mg/ml) caused also a clear decrease of cell viabilities in a dose-dependent manner.
27405739	17	14	dep	0.01	2046:2049	arg1	2.5 mg/ml					2054:2062	2.5 mg/ml	2054:2062	0.01 to 2.5 mg/ml	2046:2062	AEPS treatment (from 0.01 to 2.5 mg/ml) caused also a clear decrease of cell viabilities in a dose-dependent manner.
27405739	20	15	theme	antiproliferative	2324:2340	arg1	activities					2342:2351	antioxidant and antiproliferative activities	2308:2351	antioxidant and antiproliferative activities	2308:2351	AEPS exhibits antioxidant and antiproliferative activities.
27405739	22	16	theme	active	2478:2483	arg1	polysaccharides					2485:2499	the active polysaccharides	2474:2499	the active polysaccharides	2474:2499	Further work will identify and purify the active polysaccharides to enhance our understanding of their complete structure and relationships with its function.
27405739	12	17	theme	hetero-sulfated-anionic	1397:1419	arg1	polysaccharides					1421:1435	a hetero-sulfated-anionic polysaccharides	1395:1435	a hetero-sulfated-anionic polysaccharides that contain carbohydrate (52 %), uronic acids (23 %), ester sulfate (11 %) and protein (12 %)	1395:1530	AEPS is found to be a hetero-sulfated-anionic polysaccharides that contain carbohydrate (52 %), uronic acids (23 %), ester sulfate (11 %) and protein (12 %).
27405739	12	17	theme	hetero-sulfated-anionic	1397:1419	arg1	AEPS					1375:1378	AEPS	1375:1378	AEPS	1375:1378	AEPS is found to be a hetero-sulfated-anionic polysaccharides that contain carbohydrate (52 %), uronic acids (23 %), ester sulfate (11 %) and protein (12 %).
27405739	18	18	theme	IC50	2146:2149	arg1	values					2151:2156	The IC50 values	2142:2156	The IC50 values obtained in HepG2 and Caco-2 cells	2142:2191	The IC50 values obtained in HepG2 and Caco-2 cells were 1.06 mg/ml and 0.3 mg/ml respectively.
27405739	18	18	theme	IC50	2146:2149	arg1	1.06 mg/ml					2198:2207	1.06 mg/ml	2198:2207	1.06 mg/ml	2198:2207	The IC50 values obtained in HepG2 and Caco-2 cells were 1.06 mg/ml and 0.3 mg/ml respectively.
27405739	22	19	theme	Further	2436:2442	arg1	work					2444:2447	Further work	2436:2447	Further work	2436:2447	Further work will identify and purify the active polysaccharides to enhance our understanding of their complete structure and relationships with its function.
27405739	19	20	dep	CONCLUSIONS	2237:2247	arg1	evidenced					2260:2268	evidenced	2260:2268	evidenced that the Graesiella sp	2260:2291	CONCLUSIONS This study evidenced that the Graesiella sp.
27405739	21	21	theme	structural	2415:2424	arg1	features					2426:2433	its fine structural features	2406:2433	its fine structural features	2406:2433	The biological activities of this extract depend on its fine structural features.
27405739	2	22	theme	thermal	262:268	arg1	"					283:283	thermal baths "Hammam"	262:283	thermal baths "Hammam"	262:283	Indeed, thermal baths "Hammam" were recommended in the treatment of different type of illnesses as, for instance, for relaxing joints and soothing.
27405739	6	23	used	used	784:787	arg2	parameters					768:777	METHODS Different parameters	750:777	METHODS Different parameters	750:777	METHODS Different parameters were used to characterize the AEPS.
27405739	17	24	theme	AEPS	2025:2028	arg1	treatment					2030:2038	AEPS treatment	2025:2038	AEPS treatment (from 0.01 to 2.5 mg/ml)	2025:2063	AEPS treatment (from 0.01 to 2.5 mg/ml) caused also a clear decrease of cell viabilities in a dose-dependent manner.
27405739	0	25	theme	extracellular	89:101	arg1	polysaccharides					103:117	the aqueous extracellular polysaccharides	77:117	the aqueous extracellular polysaccharides from the thermophilic microalgae Graesiella sp	77:164	Partial characterization and antioxidant and antiproliferative activities of the aqueous extracellular polysaccharides from the thermophilic microalgae Graesiella sp.
27405739	1	26	theme	years	195:199	arg1	thousands					182:190	thousands	182:190	thousands of years	182:199	BACKGROUND For thousands of years, Tunisian geothermal water has been used in bathing.
27405739	15	27	theme	radical	1911:1917	arg1	manner					1892:1897	a concentration-dependent manner	1866:1897	a concentration-dependent manner of hydroxyl radical	1866:1917	AEPS showed high activity as reducing agent, high ferrous chelating capacity and caused a significant decrease in a concentration-dependent manner of hydroxyl radical.
27405739	17	28	theme	dose-dependent	2119:2132	arg1	manner					2134:2139	a dose-dependent manner	2117:2139	a dose-dependent manner	2117:2139	AEPS treatment (from 0.01 to 2.5 mg/ml) caused also a clear decrease of cell viabilities in a dose-dependent manner.
27405739	9	29	theme	total	1104:1108	arg1	capacity					1122:1129	total antioxidant capacity	1104:1129	total antioxidant capacity	1104:1129	AEPS was analyzed for its antioxidant propriety by means of total antioxidant capacity, DPPH radicals scavenging assay, ferrous chelating ability and hydroxyl and superoxide radical scavenging activity.
27405739	16	30	theme	DPPH	1931:1934	arg1	activity					1947:1954	A moderate DPPH scavenging activity	1920:1954	A moderate DPPH scavenging activity	1920:1954	A moderate DPPH scavenging activity and a poor superoxide radical scavenging ability were also observed.
27405739	0	31	from	activities	63:72	arg1	sp					163:164	the thermophilic microalgae Graesiella sp	124:164	the thermophilic microalgae Graesiella sp	124:164	Partial characterization and antioxidant and antiproliferative activities of the aqueous extracellular polysaccharides from the thermophilic microalgae Graesiella sp.
27405739	11	32	theme	Graesiella	1360:1369	arg1	sp					1371:1372	The Graesiella sp	1356:1372	The Graesiella sp	1356:1372	RESULTS The Graesiella sp.
27405739	15	33	theme	high	1764:1767	arg1	activity					1769:1776	high activity	1764:1776	high activity	1764:1776	AEPS showed high activity as reducing agent, high ferrous chelating capacity and caused a significant decrease in a concentration-dependent manner of hydroxyl radical.
27405739	22	34	theme	structure	2548:2556	arg1	understanding					2516:2528	our understanding	2512:2528	our understanding of their complete structure and relationships with its function	2512:2592	Further work will identify and purify the active polysaccharides to enhance our understanding of their complete structure and relationships with its function.
27405739	0	35	theme	thermophilic	128:139	arg1	sp					163:164	the thermophilic microalgae Graesiella sp	124:164	the thermophilic microalgae Graesiella sp	124:164	Partial characterization and antioxidant and antiproliferative activities of the aqueous extracellular polysaccharides from the thermophilic microalgae Graesiella sp.
27405739	15	36	theme	concentration-dependent	1868:1890	arg1	manner					1892:1897	a concentration-dependent manner	1866:1897	a concentration-dependent manner of hydroxyl radical	1866:1917	AEPS showed high activity as reducing agent, high ferrous chelating capacity and caused a significant decrease in a concentration-dependent manner of hydroxyl radical.
27405739	9	37	theme	DPPH	1132:1135	arg1	radicals					1137:1144	DPPH radicals	1132:1144	DPPH radicals scavenging assay	1132:1161	AEPS was analyzed for its antioxidant propriety by means of total antioxidant capacity, DPPH radicals scavenging assay, ferrous chelating ability and hydroxyl and superoxide radical scavenging activity.
27405739	2	38	theme	relaxing	372:379	arg1	joints					381:386	relaxing joints	372:386	relaxing joints	372:386	Indeed, thermal baths "Hammam" were recommended in the treatment of different type of illnesses as, for instance, for relaxing joints and soothing.
27405739	0	39	theme	Partial	0:6	arg1	characterization					8:23	Partial characterization	0:23	Partial characterization	0:23	Partial characterization and antioxidant and antiproliferative activities of the aqueous extracellular polysaccharides from the thermophilic microalgae Graesiella sp.
27405739	9	40	theme	scavenging	1146:1155	arg1	assay					1157:1161	DPPH radicals scavenging assay	1132:1161	DPPH radicals scavenging assay	1132:1161	AEPS was analyzed for its antioxidant propriety by means of total antioxidant capacity, DPPH radicals scavenging assay, ferrous chelating ability and hydroxyl and superoxide radical scavenging activity.
27405739	3	41	theme	high	446:449	arg1	temperature					451:461	the high temperature	442:461	the high temperature	442:461	The ability of microalgae to sustain at the high temperature makes them potential producers of high value thermostable bio-products.
27405739	7	42	theme	dry	819:821	arg1	weight					823:828	The dry weight	815:828	The dry weight	815:828	The dry weight, volatile dry weight, elemental analysis, monosaccharide composition and IR-spectroscopy analysis.
27405739	16	43	theme	superoxide	1967:1976	arg1	ability					1997:2003	a poor superoxide radical scavenging ability	1960:2003	a poor superoxide radical scavenging ability	1960:2003	A moderate DPPH scavenging activity and a poor superoxide radical scavenging ability were also observed.
27405739	8	44	theme	acid	950:953	arg1	concentrations					982:995	Carbohydrate, uronic acid, ester sulfate and protein concentrations	929:995	Carbohydrate, uronic acid, ester sulfate and protein concentrations	929:995	Carbohydrate, uronic acid, ester sulfate and protein concentrations were also determined using colorimetric assay.
27405739	14	45	theme	groups	1744:1749	arg1	presence					1694:1701	the presence	1690:1701	the presence of carboxyl, hydroxyl, amine and sulfate groups	1690:1749	The FT-IR revealed the presence of carboxyl, hydroxyl, amine and sulfate groups.
27405739	8	46	theme	ester	956:960	arg1	sulfate					962:968	ester sulfate	956:968	ester sulfate	956:968	Carbohydrate, uronic acid, ester sulfate and protein concentrations were also determined using colorimetric assay.
27405739	9	47	theme	chelating	1172:1180	arg1	ability					1182:1188	ferrous chelating ability	1164:1188	ferrous chelating ability	1164:1188	AEPS was analyzed for its antioxidant propriety by means of total antioxidant capacity, DPPH radicals scavenging assay, ferrous chelating ability and hydroxyl and superoxide radical scavenging activity.
27405739	3	48	theme	potential	474:482	arg1	producers					484:492	potential producers	474:492	potential producers of high value thermostable bio-products	474:532	The ability of microalgae to sustain at the high temperature makes them potential producers of high value thermostable bio-products.
27405739	18	49	theme	Caco-2	2180:2185	arg1	cells					2187:2191	Caco-2 cells	2180:2191	Caco-2 cells	2180:2191	The IC50 values obtained in HepG2 and Caco-2 cells were 1.06 mg/ml and 0.3 mg/ml respectively.
27405739	17	50	theme	cell	2097:2100	arg1	viabilities					2102:2112	cell viabilities	2097:2112	cell viabilities	2097:2112	AEPS treatment (from 0.01 to 2.5 mg/ml) caused also a clear decrease of cell viabilities in a dose-dependent manner.
27405739	13	51	dep	sugars	1587:1592	arg1	galactose					1603:1611	galactose	1603:1611	galactose	1603:1611	The carbohydrate fraction was formed by eight neutral sugars glucose, galactose, mannose, fucose, rhamnose, xylose, arabinose and ribose.
27405739	13	51	dep	sugars	1587:1592	arg1	mannose					1614:1620	mannose	1614:1620	mannose	1614:1620	The carbohydrate fraction was formed by eight neutral sugars glucose, galactose, mannose, fucose, rhamnose, xylose, arabinose and ribose.
27405739	13	51	dep	sugars	1587:1592	arg1	xylose					1641:1646	xylose	1641:1646	xylose	1641:1646	The carbohydrate fraction was formed by eight neutral sugars glucose, galactose, mannose, fucose, rhamnose, xylose, arabinose and ribose.
27405739	13	51	dep	sugars	1587:1592	arg1	arabinose					1649:1657	arabinose	1649:1657	arabinose	1649:1657	The carbohydrate fraction was formed by eight neutral sugars glucose, galactose, mannose, fucose, rhamnose, xylose, arabinose and ribose.
27405739	13	51	dep	sugars	1587:1592	arg1	sugars					1587:1592	eight neutral sugars glucose, galactose, mannose, fucose, rhamnose, xylose, arabinose and ribose	1573:1668	eight neutral sugars glucose, galactose, mannose, fucose, rhamnose, xylose, arabinose and ribose	1573:1668	The carbohydrate fraction was formed by eight neutral sugars glucose, galactose, mannose, fucose, rhamnose, xylose, arabinose and ribose.
27405739	13	51	dep	sugars	1587:1592	arg1	glucose					1594:1600	glucose	1594:1600	glucose	1594:1600	The carbohydrate fraction was formed by eight neutral sugars glucose, galactose, mannose, fucose, rhamnose, xylose, arabinose and ribose.
27405739	13	51	dep	sugars	1587:1592	arg1	fucose					1623:1628	fucose	1623:1628	fucose	1623:1628	The carbohydrate fraction was formed by eight neutral sugars glucose, galactose, mannose, fucose, rhamnose, xylose, arabinose and ribose.
27405739	13	51	dep	sugars	1587:1592	arg1	rhamnose					1631:1638	rhamnose	1631:1638	rhamnose	1631:1638	The carbohydrate fraction was formed by eight neutral sugars glucose, galactose, mannose, fucose, rhamnose, xylose, arabinose and ribose.
27405739	13	51	dep	sugars	1587:1592	arg1	ribose					1663:1668	ribose	1663:1668	ribose	1663:1668	The carbohydrate fraction was formed by eight neutral sugars glucose, galactose, mannose, fucose, rhamnose, xylose, arabinose and ribose.
27405739	4	52	theme	aqueous	596:602	arg1	AEPS					635:638	AEPS	635:638	AEPS	635:638	This study aimed to explore the therapeutic potential of the aqueous extracellular polysaccharides (AEPS) of the Tunisian thermophilic microalgae Graesiella sp.
27405739	4	52	theme	aqueous	596:602	arg1	polysaccharides					618:632	the aqueous extracellular polysaccharides	592:632	the aqueous extracellular polysaccharides (AEPS)	592:639	This study aimed to explore the therapeutic potential of the aqueous extracellular polysaccharides (AEPS) of the Tunisian thermophilic microalgae Graesiella sp.
27405739	12	53	contain	contain	1442:1448	arg2	%					1488:1488	23 %	1485:1488	23 %	1485:1488	AEPS is found to be a hetero-sulfated-anionic polysaccharides that contain carbohydrate (52 %), uronic acids (23 %), ester sulfate (11 %) and protein (12 %).
27405739	12	53	contain	contain	1442:1448	arg2	%					1467:1467	52 %	1464:1467	52 %	1464:1467	AEPS is found to be a hetero-sulfated-anionic polysaccharides that contain carbohydrate (52 %), uronic acids (23 %), ester sulfate (11 %) and protein (12 %).
27405739	12	53	contain	contain	1442:1448	arg2	%					1510:1510	11 %	1507:1510	11 %	1507:1510	AEPS is found to be a hetero-sulfated-anionic polysaccharides that contain carbohydrate (52 %), uronic acids (23 %), ester sulfate (11 %) and protein (12 %).
27405739	12	53	contain	contain	1442:1448	arg2	%					1529:1529	12 %	1526:1529	12 %	1526:1529	AEPS is found to be a hetero-sulfated-anionic polysaccharides that contain carbohydrate (52 %), uronic acids (23 %), ester sulfate (11 %) and protein (12 %).
27405739	12	53	contain	contain	1442:1448	arg2	protein					1517:1523	protein	1517:1523	protein (12 %)	1517:1530	AEPS is found to be a hetero-sulfated-anionic polysaccharides that contain carbohydrate (52 %), uronic acids (23 %), ester sulfate (11 %) and protein (12 %).
27405739	12	53	contain	contain	1442:1448	arg2	carbohydrate					1450:1461	carbohydrate	1450:1461	carbohydrate (52 %)	1450:1468	AEPS is found to be a hetero-sulfated-anionic polysaccharides that contain carbohydrate (52 %), uronic acids (23 %), ester sulfate (11 %) and protein (12 %).
27405739	12	53	contain	contain	1442:1448	arg1	AEPS					1375:1378	AEPS	1375:1378	AEPS	1375:1378	AEPS is found to be a hetero-sulfated-anionic polysaccharides that contain carbohydrate (52 %), uronic acids (23 %), ester sulfate (11 %) and protein (12 %).
27405739	12	53	contain	contain	1442:1448	arg1	polysaccharides					1421:1435	a hetero-sulfated-anionic polysaccharides	1395:1435	a hetero-sulfated-anionic polysaccharides that contain carbohydrate (52 %), uronic acids (23 %), ester sulfate (11 %) and protein (12 %)	1395:1530	AEPS is found to be a hetero-sulfated-anionic polysaccharides that contain carbohydrate (52 %), uronic acids (23 %), ester sulfate (11 %) and protein (12 %).
27405739	12	53	contain	contain	1442:1448	arg2	sulfate					1498:1504	ester sulfate	1492:1504	ester sulfate (11 %)	1492:1511	AEPS is found to be a hetero-sulfated-anionic polysaccharides that contain carbohydrate (52 %), uronic acids (23 %), ester sulfate (11 %) and protein (12 %).
27405739	12	53	contain	contain	1442:1448	arg2	acids					1478:1482	uronic acids	1471:1482	uronic acids (23 %)	1471:1489	AEPS is found to be a hetero-sulfated-anionic polysaccharides that contain carbohydrate (52 %), uronic acids (23 %), ester sulfate (11 %) and protein (12 %).
27405739	6	54	theme	Different	758:766	arg1	parameters					768:777	METHODS Different parameters	750:777	METHODS Different parameters	750:777	METHODS Different parameters were used to characterize the AEPS.
27405739	3	55	theme	value	502:506	arg1	bio-products					521:532	high value thermostable bio-products	497:532	high value thermostable bio-products	497:532	The ability of microalgae to sustain at the high temperature makes them potential producers of high value thermostable bio-products.
27405739	4	56	theme	polysaccharides	618:632	arg1	potential					579:587	the therapeutic potential	563:587	the therapeutic potential of the aqueous extracellular polysaccharides (AEPS) of the Tunisian thermophilic microalgae Graesiella sp	563:693	This study aimed to explore the therapeutic potential of the aqueous extracellular polysaccharides (AEPS) of the Tunisian thermophilic microalgae Graesiella sp.
27405739	9	57	theme	radical	1218:1224	arg1	activity					1237:1244	hydroxyl and superoxide radical scavenging activity	1194:1244	hydroxyl and superoxide radical scavenging activity	1194:1244	AEPS was analyzed for its antioxidant propriety by means of total antioxidant capacity, DPPH radicals scavenging assay, ferrous chelating ability and hydroxyl and superoxide radical scavenging activity.
27405739	22	58	theme	relationships	2562:2574	arg1	understanding					2516:2528	our understanding	2512:2528	our understanding of their complete structure and relationships with its function	2512:2592	Further work will identify and purify the active polysaccharides to enhance our understanding of their complete structure and relationships with its function.
27405739	10	59	theme	AEPS	1281:1284	arg1	activity					1269:1276	The antiproliferative activity	1247:1276	The antiproliferative activity of AEPS	1247:1284	The antiproliferative activity of AEPS was evaluated for HepG2 and Caco-2 cells using the MTT assay.
27405739	4	60	theme	therapeutic	567:577	arg1	potential					579:587	the therapeutic potential	563:587	the therapeutic potential of the aqueous extracellular polysaccharides (AEPS) of the Tunisian thermophilic microalgae Graesiella sp	563:693	This study aimed to explore the therapeutic potential of the aqueous extracellular polysaccharides (AEPS) of the Tunisian thermophilic microalgae Graesiella sp.
27405739	15	61	theme	reducing	1781:1788	arg1	capacity					1820:1827	high ferrous chelating capacity	1797:1827	high ferrous chelating capacity	1797:1827	AEPS showed high activity as reducing agent, high ferrous chelating capacity and caused a significant decrease in a concentration-dependent manner of hydroxyl radical.
27405739	15	61	theme	reducing	1781:1788	arg1	agent					1790:1794	reducing agent	1781:1794	reducing agent	1781:1794	AEPS showed high activity as reducing agent, high ferrous chelating capacity and caused a significant decrease in a concentration-dependent manner of hydroxyl radical.
27405739	13	62	theme	neutral	1579:1585	arg1	galactose					1603:1611	galactose	1603:1611	galactose	1603:1611	The carbohydrate fraction was formed by eight neutral sugars glucose, galactose, mannose, fucose, rhamnose, xylose, arabinose and ribose.
27405739	13	62	theme	neutral	1579:1585	arg1	mannose					1614:1620	mannose	1614:1620	mannose	1614:1620	The carbohydrate fraction was formed by eight neutral sugars glucose, galactose, mannose, fucose, rhamnose, xylose, arabinose and ribose.
27405739	13	62	theme	neutral	1579:1585	arg1	xylose					1641:1646	xylose	1641:1646	xylose	1641:1646	The carbohydrate fraction was formed by eight neutral sugars glucose, galactose, mannose, fucose, rhamnose, xylose, arabinose and ribose.
27405739	13	62	theme	neutral	1579:1585	arg1	arabinose					1649:1657	arabinose	1649:1657	arabinose	1649:1657	The carbohydrate fraction was formed by eight neutral sugars glucose, galactose, mannose, fucose, rhamnose, xylose, arabinose and ribose.
27405739	13	62	theme	neutral	1579:1585	arg1	sugars					1587:1592	eight neutral sugars glucose, galactose, mannose, fucose, rhamnose, xylose, arabinose and ribose	1573:1668	eight neutral sugars glucose, galactose, mannose, fucose, rhamnose, xylose, arabinose and ribose	1573:1668	The carbohydrate fraction was formed by eight neutral sugars glucose, galactose, mannose, fucose, rhamnose, xylose, arabinose and ribose.
27405739	13	62	theme	neutral	1579:1585	arg1	glucose					1594:1600	glucose	1594:1600	glucose	1594:1600	The carbohydrate fraction was formed by eight neutral sugars glucose, galactose, mannose, fucose, rhamnose, xylose, arabinose and ribose.
27405739	13	62	theme	neutral	1579:1585	arg1	fucose					1623:1628	fucose	1623:1628	fucose	1623:1628	The carbohydrate fraction was formed by eight neutral sugars glucose, galactose, mannose, fucose, rhamnose, xylose, arabinose and ribose.
27405739	13	62	theme	neutral	1579:1585	arg1	rhamnose					1631:1638	rhamnose	1631:1638	rhamnose	1631:1638	The carbohydrate fraction was formed by eight neutral sugars glucose, galactose, mannose, fucose, rhamnose, xylose, arabinose and ribose.
27405739	13	62	theme	neutral	1579:1585	arg1	ribose					1663:1668	ribose	1663:1668	ribose	1663:1668	The carbohydrate fraction was formed by eight neutral sugars glucose, galactose, mannose, fucose, rhamnose, xylose, arabinose and ribose.
27405739	7	63	theme	IR-spectroscopy	903:917	arg1	analysis					919:926	IR-spectroscopy analysis	903:926	IR-spectroscopy analysis	903:926	The dry weight, volatile dry weight, elemental analysis, monosaccharide composition and IR-spectroscopy analysis.
27405739	12	64	theme	uronic	1471:1476	arg1	acids					1478:1482	uronic acids	1471:1482	uronic acids (23 %)	1471:1489	AEPS is found to be a hetero-sulfated-anionic polysaccharides that contain carbohydrate (52 %), uronic acids (23 %), ester sulfate (11 %) and protein (12 %).
27405739	12	64	theme	uronic	1471:1476	arg1	%					1488:1488	23 %	1485:1488	23 %	1485:1488	AEPS is found to be a hetero-sulfated-anionic polysaccharides that contain carbohydrate (52 %), uronic acids (23 %), ester sulfate (11 %) and protein (12 %).
27405739	3	65	theme	bio-products	521:532	arg1	producers					484:492	potential producers	474:492	potential producers of high value thermostable bio-products	474:532	The ability of microalgae to sustain at the high temperature makes them potential producers of high value thermostable bio-products.
27405739	4	66	theme	Tunisian	648:655	arg1	sp					692:693	the Tunisian thermophilic microalgae Graesiella sp	644:693	the Tunisian thermophilic microalgae Graesiella sp	644:693	This study aimed to explore the therapeutic potential of the aqueous extracellular polysaccharides (AEPS) of the Tunisian thermophilic microalgae Graesiella sp.
27405739	2	67	theme	different	322:330	arg1	type					332:335	different type	322:335	different type of illnesses as	322:351	Indeed, thermal baths "Hammam" were recommended in the treatment of different type of illnesses as, for instance, for relaxing joints and soothing.
27405739	1	68	theme	geothermal	211:220	arg1	water					222:226	Tunisian geothermal water	202:226	Tunisian geothermal water	202:226	BACKGROUND For thousands of years, Tunisian geothermal water has been used in bathing.
27405739	4	69	theme	microalgae	670:679	arg1	sp					692:693	the Tunisian thermophilic microalgae Graesiella sp	644:693	the Tunisian thermophilic microalgae Graesiella sp	644:693	This study aimed to explore the therapeutic potential of the aqueous extracellular polysaccharides (AEPS) of the Tunisian thermophilic microalgae Graesiella sp.
27405739	15	70	theme	significant	1842:1852	arg1	decrease					1854:1861	a significant decrease	1840:1861	a significant decrease	1840:1861	AEPS showed high activity as reducing agent, high ferrous chelating capacity and caused a significant decrease in a concentration-dependent manner of hydroxyl radical.
27405739	7	71	theme	elemental	852:860	arg1	analysis					862:869	elemental analysis	852:869	elemental analysis	852:869	The dry weight, volatile dry weight, elemental analysis, monosaccharide composition and IR-spectroscopy analysis.
27405739	5	72	theme	physico-chemical	716:731	arg1	characteristics					733:747	its physico-chemical characteristics	712:747	its physico-chemical characteristics	712:747	and to evaluate its physico-chemical characteristics.
27405739	1	73	used	used	237:240	arg2	water					222:226	Tunisian geothermal water	202:226	Tunisian geothermal water	202:226	BACKGROUND For thousands of years, Tunisian geothermal water has been used in bathing.
27405739	13	74	theme	carbohydrate	1537:1548	arg1	fraction					1550:1557	The carbohydrate fraction	1533:1557	The carbohydrate fraction	1533:1557	The carbohydrate fraction was formed by eight neutral sugars glucose, galactose, mannose, fucose, rhamnose, xylose, arabinose and ribose.
27405739	2	75	theme	Hammam	277:282	arg1	"					283:283	thermal baths "Hammam"	262:283	thermal baths "Hammam"	262:283	Indeed, thermal baths "Hammam" were recommended in the treatment of different type of illnesses as, for instance, for relaxing joints and soothing.
27405739	21	76	theme	extract	2388:2394	arg1	activities					2369:2378	The biological activities	2354:2378	The biological activities of this extract	2354:2394	The biological activities of this extract depend on its fine structural features.
27405739	9	77	theme	antioxidant	1070:1080	arg1	propriety					1082:1090	its antioxidant propriety	1066:1090	its antioxidant propriety	1066:1090	AEPS was analyzed for its antioxidant propriety by means of total antioxidant capacity, DPPH radicals scavenging assay, ferrous chelating ability and hydroxyl and superoxide radical scavenging activity.
27405739	0	78	theme	aqueous	81:87	arg1	polysaccharides					103:117	the aqueous extracellular polysaccharides	77:117	the aqueous extracellular polysaccharides from the thermophilic microalgae Graesiella sp	77:164	Partial characterization and antioxidant and antiproliferative activities of the aqueous extracellular polysaccharides from the thermophilic microalgae Graesiella sp.
27405739	2	79	theme	baths	270:274	arg1	"					283:283	thermal baths "Hammam"	262:283	thermal baths "Hammam"	262:283	Indeed, thermal baths "Hammam" were recommended in the treatment of different type of illnesses as, for instance, for relaxing joints and soothing.
27405739	4	80	theme	sp	692:693	arg1	potential					579:587	the therapeutic potential	563:587	the therapeutic potential of the aqueous extracellular polysaccharides (AEPS) of the Tunisian thermophilic microalgae Graesiella sp	563:693	This study aimed to explore the therapeutic potential of the aqueous extracellular polysaccharides (AEPS) of the Tunisian thermophilic microalgae Graesiella sp.
27405739	0	81	theme	polysaccharides	103:117	arg1	activities					63:72	antioxidant and antiproliferative activities	29:72	antioxidant and antiproliferative activities	29:72	Partial characterization and antioxidant and antiproliferative activities of the aqueous extracellular polysaccharides from the thermophilic microalgae Graesiella sp.
27405739	0	81	theme	polysaccharides	103:117	arg1	characterization					8:23	Partial characterization	0:23	Partial characterization	0:23	Partial characterization and antioxidant and antiproliferative activities of the aqueous extracellular polysaccharides from the thermophilic microalgae Graesiella sp.
27405739	0	82	from	characterization	8:23	arg1	sp					163:164	the thermophilic microalgae Graesiella sp	124:164	the thermophilic microalgae Graesiella sp	124:164	Partial characterization and antioxidant and antiproliferative activities of the aqueous extracellular polysaccharides from the thermophilic microalgae Graesiella sp.
27405739	21	83	theme	biological	2358:2367	arg1	activities					2369:2378	The biological activities	2354:2378	The biological activities of this extract	2354:2394	The biological activities of this extract depend on its fine structural features.
27405739	10	84	theme	Caco-2	1314:1319	arg1	cells					1321:1325	Caco-2 cells	1314:1325	Caco-2 cells	1314:1325	The antiproliferative activity of AEPS was evaluated for HepG2 and Caco-2 cells using the MTT assay.
27405739	16	85	theme	scavenging	1936:1945	arg1	activity					1947:1954	A moderate DPPH scavenging activity	1920:1954	A moderate DPPH scavenging activity	1920:1954	A moderate DPPH scavenging activity and a poor superoxide radical scavenging ability were also observed.
27405739	17	86	from	0.01	2046:2049	arg1	treatment					2030:2038	AEPS treatment	2025:2038	AEPS treatment (from 0.01 to 2.5 mg/ml)	2025:2063	AEPS treatment (from 0.01 to 2.5 mg/ml) caused also a clear decrease of cell viabilities in a dose-dependent manner.
27405739	1	87	dep	BACKGROUND	167:176	arg1	used					237:240	used	237:240	has been used in bathing	228:251	BACKGROUND For thousands of years, Tunisian geothermal water has been used in bathing.
27405739	9	88	theme	antioxidant	1110:1120	arg1	capacity					1122:1129	total antioxidant capacity	1104:1129	total antioxidant capacity	1104:1129	AEPS was analyzed for its antioxidant propriety by means of total antioxidant capacity, DPPH radicals scavenging assay, ferrous chelating ability and hydroxyl and superoxide radical scavenging activity.
27405739	0	89	theme	microalgae	141:150	arg1	sp					163:164	the thermophilic microalgae Graesiella sp	124:164	the thermophilic microalgae Graesiella sp	124:164	Partial characterization and antioxidant and antiproliferative activities of the aqueous extracellular polysaccharides from the thermophilic microalgae Graesiella sp.
27405739	21	90	theme	fine	2410:2413	arg1	features					2426:2433	its fine structural features	2406:2433	its fine structural features	2406:2433	The biological activities of this extract depend on its fine structural features.
27405739	15	91	theme	hydroxyl	1902:1909	arg1	radical					1911:1917	hydroxyl radical	1902:1917	hydroxyl radical	1902:1917	AEPS showed high activity as reducing agent, high ferrous chelating capacity and caused a significant decrease in a concentration-dependent manner of hydroxyl radical.
27405739	10	92	theme	MTT	1337:1339	arg1	assay					1341:1345	the MTT assay	1333:1345	the MTT assay	1333:1345	The antiproliferative activity of AEPS was evaluated for HepG2 and Caco-2 cells using the MTT assay.
27405739	22	93	theme	complete	2539:2546	arg1	structure					2548:2556	their complete structure	2533:2556	their complete structure	2533:2556	Further work will identify and purify the active polysaccharides to enhance our understanding of their complete structure and relationships with its function.
27405739	15	94	theme	ferrous	1802:1808	arg1	capacity					1820:1827	high ferrous chelating capacity	1797:1827	high ferrous chelating capacity	1797:1827	AEPS showed high activity as reducing agent, high ferrous chelating capacity and caused a significant decrease in a concentration-dependent manner of hydroxyl radical.
27405739	15	94	theme	ferrous	1802:1808	arg1	agent					1790:1794	reducing agent	1781:1794	reducing agent	1781:1794	AEPS showed high activity as reducing agent, high ferrous chelating capacity and caused a significant decrease in a concentration-dependent manner of hydroxyl radical.
27405739	16	95	theme	poor	1962:1965	arg1	ability					1997:2003	a poor superoxide radical scavenging ability	1960:2003	a poor superoxide radical scavenging ability	1960:2003	A moderate DPPH scavenging activity and a poor superoxide radical scavenging ability were also observed.
27405739	9	96	theme	radicals	1137:1144	arg1	assay					1157:1161	DPPH radicals scavenging assay	1132:1161	DPPH radicals scavenging assay	1132:1161	AEPS was analyzed for its antioxidant propriety by means of total antioxidant capacity, DPPH radicals scavenging assay, ferrous chelating ability and hydroxyl and superoxide radical scavenging activity.
27405739	3	97	theme	microalgae	417:426	arg1	ability					406:412	The ability	402:412	The ability of microalgae to sustain at the high temperature	402:461	The ability of microalgae to sustain at the high temperature makes them potential producers of high value thermostable bio-products.
27405739	0	98	theme	antioxidant	29:39	arg1	activities					63:72	antioxidant and antiproliferative activities	29:72	antioxidant and antiproliferative activities	29:72	Partial characterization and antioxidant and antiproliferative activities of the aqueous extracellular polysaccharides from the thermophilic microalgae Graesiella sp.
27405739	7	99	theme	volatile	831:838	arg1	weight					844:849	volatile dry weight	831:849	volatile dry weight	831:849	The dry weight, volatile dry weight, elemental analysis, monosaccharide composition and IR-spectroscopy analysis.
27405739	16	100	theme	radical	1978:1984	arg1	ability					1997:2003	a poor superoxide radical scavenging ability	1960:2003	a poor superoxide radical scavenging ability	1960:2003	A moderate DPPH scavenging activity and a poor superoxide radical scavenging ability were also observed.
27405739	8	101	theme	Carbohydrate	929:940	arg1	concentrations					982:995	Carbohydrate, uronic acid, ester sulfate and protein concentrations	929:995	Carbohydrate, uronic acid, ester sulfate and protein concentrations	929:995	Carbohydrate, uronic acid, ester sulfate and protein concentrations were also determined using colorimetric assay.
27405739	16	102	theme	scavenging	1986:1995	arg1	ability					1997:2003	a poor superoxide radical scavenging ability	1960:2003	a poor superoxide radical scavenging ability	1960:2003	A moderate DPPH scavenging activity and a poor superoxide radical scavenging ability were also observed.
27405739	0	103	theme	antiproliferative	45:61	arg1	activities					63:72	antioxidant and antiproliferative activities	29:72	antioxidant and antiproliferative activities	29:72	Partial characterization and antioxidant and antiproliferative activities of the aqueous extracellular polysaccharides from the thermophilic microalgae Graesiella sp.
27405739	8	104	theme	uronic	943:948	arg1	acid					950:953	uronic acid	943:953	uronic acid	943:953	Carbohydrate, uronic acid, ester sulfate and protein concentrations were also determined using colorimetric assay.
27405739	22	105	with	structure	2548:2556	arg1	function					2585:2592	its function	2581:2592	its function	2581:2592	Further work will identify and purify the active polysaccharides to enhance our understanding of their complete structure and relationships with its function.
27405739	17	106	theme	clear	2079:2083	arg1	decrease					2085:2092	a clear decrease	2077:2092	a clear decrease of cell viabilities	2077:2112	AEPS treatment (from 0.01 to 2.5 mg/ml) caused also a clear decrease of cell viabilities in a dose-dependent manner.
27405739	9	107	theme	ferrous	1164:1170	arg1	ability					1182:1188	ferrous chelating ability	1164:1188	ferrous chelating ability	1164:1188	AEPS was analyzed for its antioxidant propriety by means of total antioxidant capacity, DPPH radicals scavenging assay, ferrous chelating ability and hydroxyl and superoxide radical scavenging activity.
27405739	11	108	dep	sp	1371:1372	arg1	RESULTS					1348:1354	RESULTS	1348:1354	RESULTS	1348:1354	RESULTS The Graesiella sp.
27405739	2	109	theme	illnesses	340:348	arg1	type					332:335	different type	322:335	different type of illnesses as	322:351	Indeed, thermal baths "Hammam" were recommended in the treatment of different type of illnesses as, for instance, for relaxing joints and soothing.
27405739	12	110	theme	ester	1492:1496	arg1	sulfate					1498:1504	ester sulfate	1492:1504	ester sulfate (11 %)	1492:1511	AEPS is found to be a hetero-sulfated-anionic polysaccharides that contain carbohydrate (52 %), uronic acids (23 %), ester sulfate (11 %) and protein (12 %).
27405739	12	110	theme	ester	1492:1496	arg1	%					1510:1510	11 %	1507:1510	11 %	1507:1510	AEPS is found to be a hetero-sulfated-anionic polysaccharides that contain carbohydrate (52 %), uronic acids (23 %), ester sulfate (11 %) and protein (12 %).
27405739	8	111	theme	sulfate	962:968	arg1	concentrations					982:995	Carbohydrate, uronic acid, ester sulfate and protein concentrations	929:995	Carbohydrate, uronic acid, ester sulfate and protein concentrations	929:995	Carbohydrate, uronic acid, ester sulfate and protein concentrations were also determined using colorimetric assay.
27405739	6	112	theme	METHODS	750:756	arg1	parameters					768:777	METHODS Different parameters	750:777	METHODS Different parameters	750:777	METHODS Different parameters were used to characterize the AEPS.
27405739	4	113	theme	extracellular	604:616	arg1	AEPS					635:638	AEPS	635:638	AEPS	635:638	This study aimed to explore the therapeutic potential of the aqueous extracellular polysaccharides (AEPS) of the Tunisian thermophilic microalgae Graesiella sp.
27405739	4	113	theme	extracellular	604:616	arg1	polysaccharides					618:632	the aqueous extracellular polysaccharides	592:632	the aqueous extracellular polysaccharides (AEPS)	592:639	This study aimed to explore the therapeutic potential of the aqueous extracellular polysaccharides (AEPS) of the Tunisian thermophilic microalgae Graesiella sp.
27405739	17	114	theme	viabilities	2102:2112	arg1	decrease					2085:2092	a clear decrease	2077:2092	a clear decrease of cell viabilities	2077:2112	AEPS treatment (from 0.01 to 2.5 mg/ml) caused also a clear decrease of cell viabilities in a dose-dependent manner.
27405739	9	115	theme	hydroxyl	1194:1201	arg1	radical					1218:1224	hydroxyl and superoxide radical	1194:1224	radical	1218:1224	AEPS was analyzed for its antioxidant propriety by means of total antioxidant capacity, DPPH radicals scavenging assay, ferrous chelating ability and hydroxyl and superoxide radical scavenging activity.
27405739	2	116	theme	type	332:335	arg1	treatment					309:317	the treatment	305:317	the treatment of different type of illnesses as	305:351	Indeed, thermal baths "Hammam" were recommended in the treatment of different type of illnesses as, for instance, for relaxing joints and soothing.
27405739	19	117	theme	Graesiella	2279:2288	arg1	sp					2290:2291	the Graesiella sp	2275:2291	the Graesiella sp	2275:2291	CONCLUSIONS This study evidenced that the Graesiella sp.
27405739	16	118	theme	moderate	1922:1929	arg1	activity					1947:1954	A moderate DPPH scavenging activity	1920:1954	A moderate DPPH scavenging activity	1920:1954	A moderate DPPH scavenging activity and a poor superoxide radical scavenging ability were also observed.
27405739	8	119	theme	protein	974:980	arg1	concentrations					982:995	Carbohydrate, uronic acid, ester sulfate and protein concentrations	929:995	Carbohydrate, uronic acid, ester sulfate and protein concentrations	929:995	Carbohydrate, uronic acid, ester sulfate and protein concentrations were also determined using colorimetric assay.
27405739	0	120	from	sp	163:164	arg1	polysaccharides					103:117	the aqueous extracellular polysaccharides	77:117	the aqueous extracellular polysaccharides from the thermophilic microalgae Graesiella sp	77:164	Partial characterization and antioxidant and antiproliferative activities of the aqueous extracellular polysaccharides from the thermophilic microalgae Graesiella sp.
27405739	0	120	from	sp	163:164	arg1	activities					63:72	antioxidant and antiproliferative activities	29:72	antioxidant and antiproliferative activities	29:72	Partial characterization and antioxidant and antiproliferative activities of the aqueous extracellular polysaccharides from the thermophilic microalgae Graesiella sp.
27405739	0	120	from	sp	163:164	arg1	characterization					8:23	Partial characterization	0:23	Partial characterization	0:23	Partial characterization and antioxidant and antiproliferative activities of the aqueous extracellular polysaccharides from the thermophilic microalgae Graesiella sp.
27405739	15	121	theme	chelating	1810:1818	arg1	capacity					1820:1827	high ferrous chelating capacity	1797:1827	high ferrous chelating capacity	1797:1827	AEPS showed high activity as reducing agent, high ferrous chelating capacity and caused a significant decrease in a concentration-dependent manner of hydroxyl radical.
27405739	15	121	theme	chelating	1810:1818	arg1	agent					1790:1794	reducing agent	1781:1794	reducing agent	1781:1794	AEPS showed high activity as reducing agent, high ferrous chelating capacity and caused a significant decrease in a concentration-dependent manner of hydroxyl radical.
27405739	9	122	theme	superoxide	1207:1216	arg1	radical					1218:1224	hydroxyl and superoxide radical	1194:1224	radical	1218:1224	AEPS was analyzed for its antioxidant propriety by means of total antioxidant capacity, DPPH radicals scavenging assay, ferrous chelating ability and hydroxyl and superoxide radical scavenging activity.
27405739	3	123	theme	thermostable	508:519	arg1	bio-products					521:532	high value thermostable bio-products	497:532	high value thermostable bio-products	497:532	The ability of microalgae to sustain at the high temperature makes them potential producers of high value thermostable bio-products.
27405739	14	124	theme	carboxyl	1706:1713	arg1	sulfate					1736:1742	sulfate	1736:1742	sulfate	1736:1742	The FT-IR revealed the presence of carboxyl, hydroxyl, amine and sulfate groups.
27405739	14	124	theme	carboxyl	1706:1713	arg1	amine					1726:1730	amine	1726:1730	amine	1726:1730	The FT-IR revealed the presence of carboxyl, hydroxyl, amine and sulfate groups.
27405739	14	124	theme	carboxyl	1706:1713	arg1	groups					1744:1749	carboxyl, hydroxyl, amine and sulfate groups	1706:1749	groups	1744:1749	The FT-IR revealed the presence of carboxyl, hydroxyl, amine and sulfate groups.
27052820	6	0	theme	suitable	960:967	arg1	CNTs					969:972	suitable CNTs	960:972	suitable CNTs	960:972	Finally, suitable CNTs and surface functionalization of CNTs were selected for making the PP/CNTs composites.
27052820	3	1	theme	Different	523:531	arg1	CNTs					533:536	Different CNTs	523:536	Different CNTs	523:536	Different CNTs were incorporated into PP by solution blending.
27052820	1	2	theme	surface	267:273	arg1	functionalization					275:291	different surface functionalization	257:291	different surface functionalization of CNTs	257:299	This study investigates the efficiency of carbon nanotubes (CNTs) as reinforcement for polypropylene (PP) for biocompatible application as a function of different surface functionalization of CNTs.
27052820	2	3	theme	carbon	377:382	arg1	nanotubes					384:392	single- and multi-walled carbon nanotubes	352:392	single- and multi-walled carbon nanotubes (SWCNTs, MWCNTs)	352:409	PP composites were reinforced using various CNTs: single- and multi-walled carbon nanotubes (SWCNTs, MWCNTs), SWCNTs were covalently functionalized by plasma, for comparison, the MWCNTs were functionalized noncovalently.
27052820	2	3	theme	carbon	377:382	arg1	SWCNTs					395:400	SWCNTs	395:400	SWCNTs	395:400	PP composites were reinforced using various CNTs: single- and multi-walled carbon nanotubes (SWCNTs, MWCNTs), SWCNTs were covalently functionalized by plasma, for comparison, the MWCNTs were functionalized noncovalently.
27052820	1	4	theme	functionalization	275:291	arg1	efficiency					132:141	the efficiency	128:141	the efficiency of carbon nanotubes (CNTs) as reinforcement for polypropylene (PP) for biocompatible application	128:238	This study investigates the efficiency of carbon nanotubes (CNTs) as reinforcement for polypropylene (PP) for biocompatible application as a function of different surface functionalization of CNTs.
27052820	1	4	theme	functionalization	275:291	arg1	function					245:252	a function	243:252	a function of different surface functionalization of CNTs	243:299	This study investigates the efficiency of carbon nanotubes (CNTs) as reinforcement for polypropylene (PP) for biocompatible application as a function of different surface functionalization of CNTs.
27052820	5	5	theme	groups	781:786	arg1	Differences					700:710	Differences	700:710	Differences in nanostructure	700:727	Differences in nanostructure and the chemical compositions, number of functional groups, and structural defects for the CNTs may be the key factors affecting the mechanical properties and biocompatibility of PP nanocomposites compared to the neat PP.
27052820	5	5	theme	groups	781:786	arg1	compositions					746:757	the chemical compositions	733:757	the chemical compositions	733:757	Differences in nanostructure and the chemical compositions, number of functional groups, and structural defects for the CNTs may be the key factors affecting the mechanical properties and biocompatibility of PP nanocomposites compared to the neat PP.
27052820	5	5	theme	groups	781:786	arg1	factors					840:846	the key factors	832:846	the key factors affecting the mechanical properties and biocompatibility of PP nanocomposites	832:924	Differences in nanostructure and the chemical compositions, number of functional groups, and structural defects for the CNTs may be the key factors affecting the mechanical properties and biocompatibility of PP nanocomposites compared to the neat PP.
27052820	5	5	theme	groups	781:786	arg1	defects					804:810	structural defects	793:810	structural defects	793:810	Differences in nanostructure and the chemical compositions, number of functional groups, and structural defects for the CNTs may be the key factors affecting the mechanical properties and biocompatibility of PP nanocomposites compared to the neat PP.
27052820	5	5	theme	groups	781:786	arg1	number					760:765	number	760:765	number of functional groups	760:786	Differences in nanostructure and the chemical compositions, number of functional groups, and structural defects for the CNTs may be the key factors affecting the mechanical properties and biocompatibility of PP nanocomposites compared to the neat PP.
27052820	4	6	theme	surface	607:613	arg1	functionalization					615:631	surface functionalization	607:631	surface functionalization	607:631	The type of CNTs and surface functionalization affects the mechanical and biocompatibility results significantly.
27052820	6	7	theme	surface	978:984	arg1	functionalization					986:1002	surface functionalization	978:1002	surface functionalization of CNTs	978:1010	Finally, suitable CNTs and surface functionalization of CNTs were selected for making the PP/CNTs composites.
27052820	3	8	theme	solution	567:574	arg1	blending					576:583	solution blending	567:583	solution blending	567:583	Different CNTs were incorporated into PP by solution blending.
27052820	1	9	theme	CNTs	296:299	arg1	functionalization					275:291	different surface functionalization	257:291	different surface functionalization of CNTs	257:299	This study investigates the efficiency of carbon nanotubes (CNTs) as reinforcement for polypropylene (PP) for biocompatible application as a function of different surface functionalization of CNTs.
27052820	2	10	theme	multi-walled	364:375	arg1	nanotubes					384:392	single- and multi-walled carbon nanotubes	352:392	single- and multi-walled carbon nanotubes (SWCNTs, MWCNTs)	352:409	PP composites were reinforced using various CNTs: single- and multi-walled carbon nanotubes (SWCNTs, MWCNTs), SWCNTs were covalently functionalized by plasma, for comparison, the MWCNTs were functionalized noncovalently.
27052820	2	10	theme	multi-walled	364:375	arg1	SWCNTs					395:400	SWCNTs	395:400	SWCNTs	395:400	PP composites were reinforced using various CNTs: single- and multi-walled carbon nanotubes (SWCNTs, MWCNTs), SWCNTs were covalently functionalized by plasma, for comparison, the MWCNTs were functionalized noncovalently.
27052820	2	11	theme	PP	302:303	arg1	composites					305:314	PP composites	302:314	PP composites	302:314	PP composites were reinforced using various CNTs: single- and multi-walled carbon nanotubes (SWCNTs, MWCNTs), SWCNTs were covalently functionalized by plasma, for comparison, the MWCNTs were functionalized noncovalently.
27052820	5	12	theme	PP	908:909	arg1	nanocomposites					911:924	PP nanocomposites	908:924	PP nanocomposites	908:924	Differences in nanostructure and the chemical compositions, number of functional groups, and structural defects for the CNTs may be the key factors affecting the mechanical properties and biocompatibility of PP nanocomposites compared to the neat PP.
27052820	5	13	theme	key	836:838	arg1	Differences					700:710	Differences	700:710	Differences in nanostructure	700:727	Differences in nanostructure and the chemical compositions, number of functional groups, and structural defects for the CNTs may be the key factors affecting the mechanical properties and biocompatibility of PP nanocomposites compared to the neat PP.
27052820	5	13	theme	key	836:838	arg1	compositions					746:757	the chemical compositions	733:757	the chemical compositions	733:757	Differences in nanostructure and the chemical compositions, number of functional groups, and structural defects for the CNTs may be the key factors affecting the mechanical properties and biocompatibility of PP nanocomposites compared to the neat PP.
27052820	5	13	theme	key	836:838	arg1	factors					840:846	the key factors	832:846	the key factors affecting the mechanical properties and biocompatibility of PP nanocomposites	832:924	Differences in nanostructure and the chemical compositions, number of functional groups, and structural defects for the CNTs may be the key factors affecting the mechanical properties and biocompatibility of PP nanocomposites compared to the neat PP.
27052820	4	14	theme	CNTs	598:601	arg1	type					590:593	The type	586:593	The type of CNTs and surface functionalization	586:631	The type of CNTs and surface functionalization affects the mechanical and biocompatibility results significantly.
27052820	5	15	from	compositions	746:757	arg1	nanostructure					715:727	nanostructure	715:727	nanostructure	715:727	Differences in nanostructure and the chemical compositions, number of functional groups, and structural defects for the CNTs may be the key factors affecting the mechanical properties and biocompatibility of PP nanocomposites compared to the neat PP.
27052820	5	16	theme	nanocomposites	911:924	arg1	biocompatibility					888:903	biocompatibility	888:903	biocompatibility	888:903	Differences in nanostructure and the chemical compositions, number of functional groups, and structural defects for the CNTs may be the key factors affecting the mechanical properties and biocompatibility of PP nanocomposites compared to the neat PP.
27052820	5	16	theme	nanocomposites	911:924	arg1	properties					873:882	mechanical properties	862:882	mechanical properties	862:882	Differences in nanostructure and the chemical compositions, number of functional groups, and structural defects for the CNTs may be the key factors affecting the mechanical properties and biocompatibility of PP nanocomposites compared to the neat PP.
27052820	0	17	theme	Mechanical	0:9	arg1	properties					11:20	Mechanical properties	0:20	Mechanical properties	0:20	Mechanical properties and biocompatibility of functionalized carbon nanotubes/polypropylene composites.
27052820	2	18	theme	various	338:344	arg1	CNTs					346:349	various CNTs	338:349	various CNTs	338:349	PP composites were reinforced using various CNTs: single- and multi-walled carbon nanotubes (SWCNTs, MWCNTs), SWCNTs were covalently functionalized by plasma, for comparison, the MWCNTs were functionalized noncovalently.
27052820	4	19	theme	biocompatibility	660:675	arg1	results					677:683	the mechanical and biocompatibility results	641:683	the mechanical and biocompatibility results	641:683	The type of CNTs and surface functionalization affects the mechanical and biocompatibility results significantly.
27052820	5	20	theme	mechanical	862:871	arg1	properties					873:882	mechanical properties	862:882	mechanical properties	862:882	Differences in nanostructure and the chemical compositions, number of functional groups, and structural defects for the CNTs may be the key factors affecting the mechanical properties and biocompatibility of PP nanocomposites compared to the neat PP.
27052820	2	21	dep	SWCNTs	395:400	arg1	MWCNTs					403:408	MWCNTs	403:408	MWCNTs	403:408	PP composites were reinforced using various CNTs: single- and multi-walled carbon nanotubes (SWCNTs, MWCNTs), SWCNTs were covalently functionalized by plasma, for comparison, the MWCNTs were functionalized noncovalently.
27052820	1	22	theme	biocompatible	214:226	arg1	application					228:238	biocompatible application	214:238	biocompatible application	214:238	This study investigates the efficiency of carbon nanotubes (CNTs) as reinforcement for polypropylene (PP) for biocompatible application as a function of different surface functionalization of CNTs.
27052820	0	23	theme	functionalized	46:59	arg1	carbon					61:66	functionalized carbon	46:66	functionalized carbon	46:66	Mechanical properties and biocompatibility of functionalized carbon nanotubes/polypropylene composites.
27052820	6	24	theme	CNTs	1007:1010	arg1	functionalization					986:1002	surface functionalization	978:1002	surface functionalization of CNTs	978:1010	Finally, suitable CNTs and surface functionalization of CNTs were selected for making the PP/CNTs composites.
27052820	6	24	theme	CNTs	1007:1010	arg1	CNTs					969:972	suitable CNTs	960:972	suitable CNTs	960:972	Finally, suitable CNTs and surface functionalization of CNTs were selected for making the PP/CNTs composites.
27052820	5	25	theme	functional	770:779	arg1	groups					781:786	functional groups	770:786	functional groups	770:786	Differences in nanostructure and the chemical compositions, number of functional groups, and structural defects for the CNTs may be the key factors affecting the mechanical properties and biocompatibility of PP nanocomposites compared to the neat PP.
27052820	6	26	theme	PP/CNTs	1041:1047	arg1	composites					1049:1058	the PP/CNTs composites	1037:1058	the PP/CNTs composites	1037:1058	Finally, suitable CNTs and surface functionalization of CNTs were selected for making the PP/CNTs composites.
27052820	5	27	theme	structural	793:802	arg1	defects					804:810	structural defects	793:810	structural defects	793:810	Differences in nanostructure and the chemical compositions, number of functional groups, and structural defects for the CNTs may be the key factors affecting the mechanical properties and biocompatibility of PP nanocomposites compared to the neat PP.
27052820	1	28	theme	carbon	146:151	arg1	CNTs					164:167	CNTs	164:167	CNTs	164:167	This study investigates the efficiency of carbon nanotubes (CNTs) as reinforcement for polypropylene (PP) for biocompatible application as a function of different surface functionalization of CNTs.
27052820	1	28	theme	carbon	146:151	arg1	nanotubes					153:161	carbon nanotubes	146:161	carbon nanotubes (CNTs)	146:168	This study investigates the efficiency of carbon nanotubes (CNTs) as reinforcement for polypropylene (PP) for biocompatible application as a function of different surface functionalization of CNTs.
27052820	0	29	theme	carbon	61:66	arg1	biocompatibility					26:41	biocompatibility	26:41	biocompatibility	26:41	Mechanical properties and biocompatibility of functionalized carbon nanotubes/polypropylene composites.
27052820	0	29	theme	carbon	61:66	arg1	properties					11:20	Mechanical properties	0:20	Mechanical properties	0:20	Mechanical properties and biocompatibility of functionalized carbon nanotubes/polypropylene composites.
27052820	5	30	from	number	760:765	arg1	nanostructure					715:727	nanostructure	715:727	nanostructure	715:727	Differences in nanostructure and the chemical compositions, number of functional groups, and structural defects for the CNTs may be the key factors affecting the mechanical properties and biocompatibility of PP nanocomposites compared to the neat PP.
27052820	1	31	theme	nanotubes	153:161	arg1	efficiency					132:141	the efficiency	128:141	the efficiency of carbon nanotubes (CNTs) as reinforcement for polypropylene (PP) for biocompatible application	128:238	This study investigates the efficiency of carbon nanotubes (CNTs) as reinforcement for polypropylene (PP) for biocompatible application as a function of different surface functionalization of CNTs.
27052820	1	31	theme	nanotubes	153:161	arg1	function					245:252	a function	243:252	a function of different surface functionalization of CNTs	243:299	This study investigates the efficiency of carbon nanotubes (CNTs) as reinforcement for polypropylene (PP) for biocompatible application as a function of different surface functionalization of CNTs.
27052820	5	32	from	Differences	700:710	arg1	nanostructure					715:727	nanostructure	715:727	nanostructure	715:727	Differences in nanostructure and the chemical compositions, number of functional groups, and structural defects for the CNTs may be the key factors affecting the mechanical properties and biocompatibility of PP nanocomposites compared to the neat PP.
27052820	5	33	theme	chemical	737:744	arg1	Differences					700:710	Differences	700:710	Differences in nanostructure	700:727	Differences in nanostructure and the chemical compositions, number of functional groups, and structural defects for the CNTs may be the key factors affecting the mechanical properties and biocompatibility of PP nanocomposites compared to the neat PP.
27052820	5	33	theme	chemical	737:744	arg1	compositions					746:757	the chemical compositions	733:757	the chemical compositions	733:757	Differences in nanostructure and the chemical compositions, number of functional groups, and structural defects for the CNTs may be the key factors affecting the mechanical properties and biocompatibility of PP nanocomposites compared to the neat PP.
27052820	5	33	theme	chemical	737:744	arg1	factors					840:846	the key factors	832:846	the key factors affecting the mechanical properties and biocompatibility of PP nanocomposites	832:924	Differences in nanostructure and the chemical compositions, number of functional groups, and structural defects for the CNTs may be the key factors affecting the mechanical properties and biocompatibility of PP nanocomposites compared to the neat PP.
27052820	2	34	theme	single-	352:358	arg1	nanotubes					384:392	single- and multi-walled carbon nanotubes	352:392	single- and multi-walled carbon nanotubes (SWCNTs, MWCNTs)	352:409	PP composites were reinforced using various CNTs: single- and multi-walled carbon nanotubes (SWCNTs, MWCNTs), SWCNTs were covalently functionalized by plasma, for comparison, the MWCNTs were functionalized noncovalently.
27052820	2	34	theme	single-	352:358	arg1	SWCNTs					395:400	SWCNTs	395:400	SWCNTs	395:400	PP composites were reinforced using various CNTs: single- and multi-walled carbon nanotubes (SWCNTs, MWCNTs), SWCNTs were covalently functionalized by plasma, for comparison, the MWCNTs were functionalized noncovalently.
27052820	5	35	from	defects	804:810	arg1	nanostructure					715:727	nanostructure	715:727	nanostructure	715:727	Differences in nanostructure and the chemical compositions, number of functional groups, and structural defects for the CNTs may be the key factors affecting the mechanical properties and biocompatibility of PP nanocomposites compared to the neat PP.
27052820	4	36	theme	functionalization	615:631	arg1	type					590:593	The type	586:593	The type of CNTs and surface functionalization	586:631	The type of CNTs and surface functionalization affects the mechanical and biocompatibility results significantly.
27052820	5	37	dep	properties	873:882	arg1	the					858:860	the	858:860	the	858:860	Differences in nanostructure and the chemical compositions, number of functional groups, and structural defects for the CNTs may be the key factors affecting the mechanical properties and biocompatibility of PP nanocomposites compared to the neat PP.
27052820	4	38	theme	mechanical	645:654	arg1	results					677:683	the mechanical and biocompatibility results	641:683	the mechanical and biocompatibility results	641:683	The type of CNTs and surface functionalization affects the mechanical and biocompatibility results significantly.
27052820	5	39	theme	neat	942:945	arg1	PP					947:948	the neat PP	938:948	the neat PP	938:948	Differences in nanostructure and the chemical compositions, number of functional groups, and structural defects for the CNTs may be the key factors affecting the mechanical properties and biocompatibility of PP nanocomposites compared to the neat PP.
27052820	1	40	theme	different	257:265	arg1	functionalization					275:291	different surface functionalization	257:291	different surface functionalization of CNTs	257:299	This study investigates the efficiency of carbon nanotubes (CNTs) as reinforcement for polypropylene (PP) for biocompatible application as a function of different surface functionalization of CNTs.
28598407	1	0	from	extraction	208:217	arg1	PRPCA					292:296	PRPCA	292:296	PRPCA	292:296	In this study, the ultrasound-assisted extraction of polysaccharides (PSA) from Pinelliae Rhizoma Praeparatum Cum Alumine (PRPCA) was optimized by response surface methodology (RSM).
28598407	1	0	from	extraction	208:217	arg1	Alumine					283:289	Alumine	283:289	Alumine	283:289	In this study, the ultrasound-assisted extraction of polysaccharides (PSA) from Pinelliae Rhizoma Praeparatum Cum Alumine (PRPCA) was optimized by response surface methodology (RSM).
28598407	8	1	theme	medicines	1503:1511	arg1	products					1484:1491	processed products	1474:1491	processed products of herbal medicines	1474:1511	Collectively, this study provides a promising strategy to obtain bioactive polysaccharides from processed products of herbal medicines.
28598407	6	2	theme	1.83:0.55:75.75:1.94:0.45	1264:1288	arg1	ratio					1255:1259	a molar ratio	1247:1259	a molar ratio of 1.83:0.55:75.75:1.94:0.45	1247:1288	Monosaccharide composition analysis indicated that PSA consisted of mannose, galactose uronic acid, glucose, galactose, arabinose with a molar ratio of 1.83:0.55:75.75:1.94:0.45.
28598407	5	3	theme	×	1087:1087	arg1	weights					1042:1048	The average molecular weights	1020:1048	The average molecular weights of the PSA	1020:1059	The average molecular weights of the PSA were estimated to be 5.34 × 10³ and 6.27 × 10⁵ Da.
28598407	5	3	theme	×	1087:1087	arg1	Da					1108:1109	5.34 × 10³ and 6.27 × 10⁵ Da	1082:1109	5.34 × 10³ and 6.27 × 10⁵ Da	1082:1109	The average molecular weights of the PSA were estimated to be 5.34 × 10³ and 6.27 × 10⁵ Da.
28598407	4	4	theme	ultrasonic	872:881	arg1	power					883:887	ultrasonic power	872:887	ultrasonic power	872:887	Results indicated that the optimal extraction conditions were as follows: the ratio of water to raw of 30 mL/g, extraction time of 46.50 min, ultrasonic temperature of 72.00 °C, and ultrasonic power of 230 W. Under these conditions, the obtained PSA yield (13.21 ± 0.37%) was closely agreed with the predicted yield by the model.
28598407	4	4	theme	ultrasonic	872:881	arg1	mL/g					796:799	30 mL/g	793:799	30 mL/g	793:799	Results indicated that the optimal extraction conditions were as follows: the ratio of water to raw of 30 mL/g, extraction time of 46.50 min, ultrasonic temperature of 72.00 °C, and ultrasonic power of 230 W. Under these conditions, the obtained PSA yield (13.21 ± 0.37%) was closely agreed with the predicted yield by the model.
28598407	8	5	theme	promising	1414:1422	arg1	strategy					1424:1431	a promising strategy	1412:1431	a promising strategy to obtain bioactive polysaccharides from processed products of herbal medicines	1412:1511	Collectively, this study provides a promising strategy to obtain bioactive polysaccharides from processed products of herbal medicines.
28598407	4	6	theme	mL/g	796:799	arg1	ratio					768:772	the ratio	764:772	the ratio of water to raw of 30 mL/g, extraction time of 46.50 min, ultrasonic temperature of 72.00 °C, and ultrasonic power of 230 W. Under these conditions, the obtained PSA yield (13.21 ± 0.37%)	764:960	Results indicated that the optimal extraction conditions were as follows: the ratio of water to raw of 30 mL/g, extraction time of 46.50 min, ultrasonic temperature of 72.00 °C, and ultrasonic power of 230 W. Under these conditions, the obtained PSA yield (13.21 ± 0.37%) was closely agreed with the predicted yield by the model.
28598407	5	7	theme	PSA	1057:1059	arg1	weights					1042:1048	The average molecular weights	1020:1048	The average molecular weights of the PSA	1020:1059	The average molecular weights of the PSA were estimated to be 5.34 × 10³ and 6.27 × 10⁵ Da.
28598407	5	7	theme	PSA	1057:1059	arg1	Da					1108:1109	5.34 × 10³ and 6.27 × 10⁵ Da	1082:1109	5.34 × 10³ and 6.27 × 10⁵ Da	1082:1109	The average molecular weights of the PSA were estimated to be 5.34 × 10³ and 6.27 × 10⁵ Da.
28598407	6	8	theme	Monosaccharide	1112:1125	arg1	analysis					1139:1146	Monosaccharide composition analysis	1112:1146	Monosaccharide composition analysis	1112:1146	Monosaccharide composition analysis indicated that PSA consisted of mannose, galactose uronic acid, glucose, galactose, arabinose with a molar ratio of 1.83:0.55:75.75:1.94:0.45.
28598407	4	9	theme	±	953:953	arg1	%					959:959	13.21 ± 0.37%	947:959	13.21 ± 0.37%	947:959	Results indicated that the optimal extraction conditions were as follows: the ratio of water to raw of 30 mL/g, extraction time of 46.50 min, ultrasonic temperature of 72.00 °C, and ultrasonic power of 230 W. Under these conditions, the obtained PSA yield (13.21 ± 0.37%) was closely agreed with the predicted yield by the model.
28598407	4	9	theme	±	953:953	arg1	yield					940:944	the obtained PSA yield	923:944	the obtained PSA yield (13.21 ± 0.37%)	923:960	Results indicated that the optimal extraction conditions were as follows: the ratio of water to raw of 30 mL/g, extraction time of 46.50 min, ultrasonic temperature of 72.00 °C, and ultrasonic power of 230 W. Under these conditions, the obtained PSA yield (13.21 ± 0.37%) was closely agreed with the predicted yield by the model.
28598407	8	10	theme	herbal	1496:1501	arg1	medicines					1503:1511	herbal medicines	1496:1511	herbal medicines	1496:1511	Collectively, this study provides a promising strategy to obtain bioactive polysaccharides from processed products of herbal medicines.
28598407	4	11	theme	°C	864:865	arg1	power					883:887	ultrasonic power	872:887	ultrasonic power	872:887	Results indicated that the optimal extraction conditions were as follows: the ratio of water to raw of 30 mL/g, extraction time of 46.50 min, ultrasonic temperature of 72.00 °C, and ultrasonic power of 230 W. Under these conditions, the obtained PSA yield (13.21 ± 0.37%) was closely agreed with the predicted yield by the model.
28598407	4	11	theme	°C	864:865	arg1	time					813:816	extraction time	802:816	extraction time of 46.50 min	802:829	Results indicated that the optimal extraction conditions were as follows: the ratio of water to raw of 30 mL/g, extraction time of 46.50 min, ultrasonic temperature of 72.00 °C, and ultrasonic power of 230 W. Under these conditions, the obtained PSA yield (13.21 ± 0.37%) was closely agreed with the predicted yield by the model.
28598407	4	11	theme	°C	864:865	arg1	temperature					843:853	ultrasonic temperature	832:853	ultrasonic temperature of 72.00 °C	832:865	Results indicated that the optimal extraction conditions were as follows: the ratio of water to raw of 30 mL/g, extraction time of 46.50 min, ultrasonic temperature of 72.00 °C, and ultrasonic power of 230 W. Under these conditions, the obtained PSA yield (13.21 ± 0.37%) was closely agreed with the predicted yield by the model.
28598407	4	11	theme	°C	864:865	arg1	mL/g					796:799	30 mL/g	793:799	30 mL/g	793:799	Results indicated that the optimal extraction conditions were as follows: the ratio of water to raw of 30 mL/g, extraction time of 46.50 min, ultrasonic temperature of 72.00 °C, and ultrasonic power of 230 W. Under these conditions, the obtained PSA yield (13.21 ± 0.37%) was closely agreed with the predicted yield by the model.
28598407	4	12	theme	extraction	802:811	arg1	mL/g					796:799	30 mL/g	793:799	30 mL/g	793:799	Results indicated that the optimal extraction conditions were as follows: the ratio of water to raw of 30 mL/g, extraction time of 46.50 min, ultrasonic temperature of 72.00 °C, and ultrasonic power of 230 W. Under these conditions, the obtained PSA yield (13.21 ± 0.37%) was closely agreed with the predicted yield by the model.
28598407	4	12	theme	extraction	802:811	arg1	time					813:816	extraction time	802:816	extraction time of 46.50 min	802:829	Results indicated that the optimal extraction conditions were as follows: the ratio of water to raw of 30 mL/g, extraction time of 46.50 min, ultrasonic temperature of 72.00 °C, and ultrasonic power of 230 W. Under these conditions, the obtained PSA yield (13.21 ± 0.37%) was closely agreed with the predicted yield by the model.
28598407	1	13	theme	ultrasound-assisted	188:206	arg1	extraction					208:217	the ultrasound-assisted extraction	184:217	the ultrasound-assisted extraction of polysaccharides (PSA) from Pinelliae Rhizoma Praeparatum Cum Alumine (PRPCA)	184:297	In this study, the ultrasound-assisted extraction of polysaccharides (PSA) from Pinelliae Rhizoma Praeparatum Cum Alumine (PRPCA) was optimized by response surface methodology (RSM).
28598407	2	14	theme	liquid	549:554	arg1	chromatography					556:569	high performance liquid chromatography	532:569	high performance liquid chromatography	532:569	The structural characteristics of PSA were analyzed by UV-vis spectroscopy, infrared spectroscopy, scanning electron microscopy, high performance gel permeation chromatography and high performance liquid chromatography, respectively.
28598407	4	15	theme	predicted	990:998	arg1	yield					1000:1004	the predicted yield	986:1004	the predicted yield by the model	986:1017	Results indicated that the optimal extraction conditions were as follows: the ratio of water to raw of 30 mL/g, extraction time of 46.50 min, ultrasonic temperature of 72.00 °C, and ultrasonic power of 230 W. Under these conditions, the obtained PSA yield (13.21 ± 0.37%) was closely agreed with the predicted yield by the model.
28598407	2	16	theme	UV-vis	407:412	arg1	spectroscopy					414:425	UV-vis spectroscopy	407:425	UV-vis spectroscopy	407:425	The structural characteristics of PSA were analyzed by UV-vis spectroscopy, infrared spectroscopy, scanning electron microscopy, high performance gel permeation chromatography and high performance liquid chromatography, respectively.
28598407	6	17	theme	uronic	1199:1204	arg1	acid					1206:1209	galactose uronic acid	1189:1209	galactose uronic acid	1189:1209	Monosaccharide composition analysis indicated that PSA consisted of mannose, galactose uronic acid, glucose, galactose, arabinose with a molar ratio of 1.83:0.55:75.75:1.94:0.45.
28598407	6	17	theme	uronic	1199:1204	arg1	mannose					1180:1186	mannose	1180:1186	mannose	1180:1186	Monosaccharide composition analysis indicated that PSA consisted of mannose, galactose uronic acid, glucose, galactose, arabinose with a molar ratio of 1.83:0.55:75.75:1.94:0.45.
28598407	3	18	theme	antimicrobial	615:627	arg1	activities					629:638	antioxidant and antimicrobial activities	599:638	antioxidant and antimicrobial activities of PSA	599:645	In addition, antioxidant and antimicrobial activities of PSA were studied by different in vitro assays.
28598407	4	19	theme	extraction	725:734	arg1	conditions					736:745	the optimal extraction conditions	713:745	the optimal extraction conditions	713:745	Results indicated that the optimal extraction conditions were as follows: the ratio of water to raw of 30 mL/g, extraction time of 46.50 min, ultrasonic temperature of 72.00 °C, and ultrasonic power of 230 W. Under these conditions, the obtained PSA yield (13.21 ± 0.37%) was closely agreed with the predicted yield by the model.
28598407	4	20	theme	PSA	936:938	arg1	%					959:959	13.21 ± 0.37%	947:959	13.21 ± 0.37%	947:959	Results indicated that the optimal extraction conditions were as follows: the ratio of water to raw of 30 mL/g, extraction time of 46.50 min, ultrasonic temperature of 72.00 °C, and ultrasonic power of 230 W. Under these conditions, the obtained PSA yield (13.21 ± 0.37%) was closely agreed with the predicted yield by the model.
28598407	4	20	theme	PSA	936:938	arg1	yield					940:944	the obtained PSA yield	923:944	the obtained PSA yield (13.21 ± 0.37%)	923:960	Results indicated that the optimal extraction conditions were as follows: the ratio of water to raw of 30 mL/g, extraction time of 46.50 min, ultrasonic temperature of 72.00 °C, and ultrasonic power of 230 W. Under these conditions, the obtained PSA yield (13.21 ± 0.37%) was closely agreed with the predicted yield by the model.
28598407	4	20	theme	PSA	936:938	arg1	mL/g					796:799	30 mL/g	793:799	30 mL/g	793:799	Results indicated that the optimal extraction conditions were as follows: the ratio of water to raw of 30 mL/g, extraction time of 46.50 min, ultrasonic temperature of 72.00 °C, and ultrasonic power of 230 W. Under these conditions, the obtained PSA yield (13.21 ± 0.37%) was closely agreed with the predicted yield by the model.
28598407	6	21	theme	galactose	1189:1197	arg1	acid					1206:1209	galactose uronic acid	1189:1209	galactose uronic acid	1189:1209	Monosaccharide composition analysis indicated that PSA consisted of mannose, galactose uronic acid, glucose, galactose, arabinose with a molar ratio of 1.83:0.55:75.75:1.94:0.45.
28598407	6	21	theme	galactose	1189:1197	arg1	mannose					1180:1186	mannose	1180:1186	mannose	1180:1186	Monosaccharide composition analysis indicated that PSA consisted of mannose, galactose uronic acid, glucose, galactose, arabinose with a molar ratio of 1.83:0.55:75.75:1.94:0.45.
28598407	4	22	theme	water	777:781	arg1	ratio					768:772	the ratio	764:772	the ratio of water to raw of 30 mL/g, extraction time of 46.50 min, ultrasonic temperature of 72.00 °C, and ultrasonic power of 230 W. Under these conditions, the obtained PSA yield (13.21 ± 0.37%)	764:960	Results indicated that the optimal extraction conditions were as follows: the ratio of water to raw of 30 mL/g, extraction time of 46.50 min, ultrasonic temperature of 72.00 °C, and ultrasonic power of 230 W. Under these conditions, the obtained PSA yield (13.21 ± 0.37%) was closely agreed with the predicted yield by the model.
28598407	1	23	theme	polysaccharides	222:236	arg1	extraction					208:217	the ultrasound-assisted extraction	184:217	the ultrasound-assisted extraction of polysaccharides (PSA) from Pinelliae Rhizoma Praeparatum Cum Alumine (PRPCA)	184:297	In this study, the ultrasound-assisted extraction of polysaccharides (PSA) from Pinelliae Rhizoma Praeparatum Cum Alumine (PRPCA) was optimized by response surface methodology (RSM).
28598407	2	24	theme	electron	460:467	arg1	microscopy					469:478	scanning electron microscopy	451:478	scanning electron microscopy	451:478	The structural characteristics of PSA were analyzed by UV-vis spectroscopy, infrared spectroscopy, scanning electron microscopy, high performance gel permeation chromatography and high performance liquid chromatography, respectively.
28598407	7	25	theme	antioxidant	1327:1337	arg1	activities					1357:1366	moderate antioxidant and antibacterial activities	1318:1366	moderate antioxidant and antibacterial activities	1318:1366	Furthermore, PSA exhibited moderate antioxidant and antibacterial activities in vitro.
28598407	3	26	theme	antioxidant	599:609	arg1	activities					629:638	antioxidant and antimicrobial activities	599:638	antioxidant and antimicrobial activities of PSA	599:645	In addition, antioxidant and antimicrobial activities of PSA were studied by different in vitro assays.
28598407	2	27	theme	scanning	451:458	arg1	microscopy					469:478	scanning electron microscopy	451:478	scanning electron microscopy	451:478	The structural characteristics of PSA were analyzed by UV-vis spectroscopy, infrared spectroscopy, scanning electron microscopy, high performance gel permeation chromatography and high performance liquid chromatography, respectively.
28598407	0	28	theme	Ultrasound-Assisted	137:155	arg1	Extraction					157:166	Ultrasound-Assisted Extraction	137:166	Ultrasound-Assisted Extraction	137:166	Extraction Optimization, Characterization, and Bioactivities of Polysaccharides from Pinelliae Rhizoma Praeparatum Cum Alumine Employing Ultrasound-Assisted Extraction.
28598407	5	29	theme	molecular	1032:1040	arg1	weights					1042:1048	The average molecular weights	1020:1048	The average molecular weights of the PSA	1020:1059	The average molecular weights of the PSA were estimated to be 5.34 × 10³ and 6.27 × 10⁵ Da.
28598407	5	29	theme	molecular	1032:1040	arg1	Da					1108:1109	5.34 × 10³ and 6.27 × 10⁵ Da	1082:1109	5.34 × 10³ and 6.27 × 10⁵ Da	1082:1109	The average molecular weights of the PSA were estimated to be 5.34 × 10³ and 6.27 × 10⁵ Da.
28598407	2	30	theme	permeation	502:511	arg1	chromatography					513:526	high performance gel permeation chromatography	481:526	high performance gel permeation chromatography	481:526	The structural characteristics of PSA were analyzed by UV-vis spectroscopy, infrared spectroscopy, scanning electron microscopy, high performance gel permeation chromatography and high performance liquid chromatography, respectively.
28598407	1	31	theme	response	316:323	arg1	methodology					333:343	response surface methodology	316:343	response surface methodology (RSM)	316:349	In this study, the ultrasound-assisted extraction of polysaccharides (PSA) from Pinelliae Rhizoma Praeparatum Cum Alumine (PRPCA) was optimized by response surface methodology (RSM).
28598407	1	31	theme	response	316:323	arg1	RSM					346:348	RSM	346:348	RSM	346:348	In this study, the ultrasound-assisted extraction of polysaccharides (PSA) from Pinelliae Rhizoma Praeparatum Cum Alumine (PRPCA) was optimized by response surface methodology (RSM).
28598407	1	32	from	Alumine	283:289	arg1	PSA					239:241	PSA	239:241	PSA	239:241	In this study, the ultrasound-assisted extraction of polysaccharides (PSA) from Pinelliae Rhizoma Praeparatum Cum Alumine (PRPCA) was optimized by response surface methodology (RSM).
28598407	1	32	from	Alumine	283:289	arg1	polysaccharides					222:236	polysaccharides	222:236	polysaccharides (PSA) from Pinelliae Rhizoma Praeparatum Cum Alumine (PRPCA)	222:297	In this study, the ultrasound-assisted extraction of polysaccharides (PSA) from Pinelliae Rhizoma Praeparatum Cum Alumine (PRPCA) was optimized by response surface methodology (RSM).
28598407	1	32	from	Alumine	283:289	arg1	extraction					208:217	the ultrasound-assisted extraction	184:217	the ultrasound-assisted extraction of polysaccharides (PSA) from Pinelliae Rhizoma Praeparatum Cum Alumine (PRPCA)	184:297	In this study, the ultrasound-assisted extraction of polysaccharides (PSA) from Pinelliae Rhizoma Praeparatum Cum Alumine (PRPCA) was optimized by response surface methodology (RSM).
28598407	3	33	dep	in	673:674	arg1	vitro					676:680	vitro	676:680	vitro	676:680	In addition, antioxidant and antimicrobial activities of PSA were studied by different in vitro assays.
28598407	4	34	theme	ultrasonic	832:841	arg1	mL/g					796:799	30 mL/g	793:799	30 mL/g	793:799	Results indicated that the optimal extraction conditions were as follows: the ratio of water to raw of 30 mL/g, extraction time of 46.50 min, ultrasonic temperature of 72.00 °C, and ultrasonic power of 230 W. Under these conditions, the obtained PSA yield (13.21 ± 0.37%) was closely agreed with the predicted yield by the model.
28598407	4	34	theme	ultrasonic	832:841	arg1	temperature					843:853	ultrasonic temperature	832:853	ultrasonic temperature of 72.00 °C	832:865	Results indicated that the optimal extraction conditions were as follows: the ratio of water to raw of 30 mL/g, extraction time of 46.50 min, ultrasonic temperature of 72.00 °C, and ultrasonic power of 230 W. Under these conditions, the obtained PSA yield (13.21 ± 0.37%) was closely agreed with the predicted yield by the model.
28598407	4	35	theme	optimal	717:723	arg1	conditions					736:745	the optimal extraction conditions	713:745	the optimal extraction conditions	713:745	Results indicated that the optimal extraction conditions were as follows: the ratio of water to raw of 30 mL/g, extraction time of 46.50 min, ultrasonic temperature of 72.00 °C, and ultrasonic power of 230 W. Under these conditions, the obtained PSA yield (13.21 ± 0.37%) was closely agreed with the predicted yield by the model.
28598407	2	36	theme	infrared	428:435	arg1	spectroscopy					437:448	infrared spectroscopy	428:448	infrared spectroscopy	428:448	The structural characteristics of PSA were analyzed by UV-vis spectroscopy, infrared spectroscopy, scanning electron microscopy, high performance gel permeation chromatography and high performance liquid chromatography, respectively.
28598407	2	37	theme	gel	498:500	arg1	chromatography					513:526	high performance gel permeation chromatography	481:526	high performance gel permeation chromatography	481:526	The structural characteristics of PSA were analyzed by UV-vis spectroscopy, infrared spectroscopy, scanning electron microscopy, high performance gel permeation chromatography and high performance liquid chromatography, respectively.
28598407	1	38	theme	surface	325:331	arg1	methodology					333:343	response surface methodology	316:343	response surface methodology (RSM)	316:349	In this study, the ultrasound-assisted extraction of polysaccharides (PSA) from Pinelliae Rhizoma Praeparatum Cum Alumine (PRPCA) was optimized by response surface methodology (RSM).
28598407	1	38	theme	surface	325:331	arg1	RSM					346:348	RSM	346:348	RSM	346:348	In this study, the ultrasound-assisted extraction of polysaccharides (PSA) from Pinelliae Rhizoma Praeparatum Cum Alumine (PRPCA) was optimized by response surface methodology (RSM).
28598407	4	39	theme	obtained	927:934	arg1	%					959:959	13.21 ± 0.37%	947:959	13.21 ± 0.37%	947:959	Results indicated that the optimal extraction conditions were as follows: the ratio of water to raw of 30 mL/g, extraction time of 46.50 min, ultrasonic temperature of 72.00 °C, and ultrasonic power of 230 W. Under these conditions, the obtained PSA yield (13.21 ± 0.37%) was closely agreed with the predicted yield by the model.
28598407	4	39	theme	obtained	927:934	arg1	yield					940:944	the obtained PSA yield	923:944	the obtained PSA yield (13.21 ± 0.37%)	923:960	Results indicated that the optimal extraction conditions were as follows: the ratio of water to raw of 30 mL/g, extraction time of 46.50 min, ultrasonic temperature of 72.00 °C, and ultrasonic power of 230 W. Under these conditions, the obtained PSA yield (13.21 ± 0.37%) was closely agreed with the predicted yield by the model.
28598407	4	39	theme	obtained	927:934	arg1	mL/g					796:799	30 mL/g	793:799	30 mL/g	793:799	Results indicated that the optimal extraction conditions were as follows: the ratio of water to raw of 30 mL/g, extraction time of 46.50 min, ultrasonic temperature of 72.00 °C, and ultrasonic power of 230 W. Under these conditions, the obtained PSA yield (13.21 ± 0.37%) was closely agreed with the predicted yield by the model.
28598407	7	40	theme	antibacterial	1343:1355	arg1	activities					1357:1366	moderate antioxidant and antibacterial activities	1318:1366	moderate antioxidant and antibacterial activities	1318:1366	Furthermore, PSA exhibited moderate antioxidant and antibacterial activities in vitro.
28598407	2	41	theme	performance	486:496	arg1	chromatography					513:526	high performance gel permeation chromatography	481:526	high performance gel permeation chromatography	481:526	The structural characteristics of PSA were analyzed by UV-vis spectroscopy, infrared spectroscopy, scanning electron microscopy, high performance gel permeation chromatography and high performance liquid chromatography, respectively.
28598407	6	42	theme	molar	1249:1253	arg1	ratio					1255:1259	a molar ratio	1247:1259	a molar ratio of 1.83:0.55:75.75:1.94:0.45	1247:1288	Monosaccharide composition analysis indicated that PSA consisted of mannose, galactose uronic acid, glucose, galactose, arabinose with a molar ratio of 1.83:0.55:75.75:1.94:0.45.
28598407	0	43	theme	Polysaccharides	64:78	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction Optimization, Characterization, and Bioactivities of Polysaccharides from Pinelliae Rhizoma Praeparatum Cum Alumine Employing Ultrasound-Assisted Extraction.
28598407	0	43	theme	Polysaccharides	64:78	arg1	Characterization					25:40	Characterization	25:40	Characterization	25:40	Extraction Optimization, Characterization, and Bioactivities of Polysaccharides from Pinelliae Rhizoma Praeparatum Cum Alumine Employing Ultrasound-Assisted Extraction.
28598407	0	43	theme	Polysaccharides	64:78	arg1	Bioactivities					47:59	Bioactivities	47:59	Bioactivities	47:59	Extraction Optimization, Characterization, and Bioactivities of Polysaccharides from Pinelliae Rhizoma Praeparatum Cum Alumine Employing Ultrasound-Assisted Extraction.
28598407	7	44	theme	moderate	1318:1325	arg1	activities					1357:1366	moderate antioxidant and antibacterial activities	1318:1366	moderate antioxidant and antibacterial activities	1318:1366	Furthermore, PSA exhibited moderate antioxidant and antibacterial activities in vitro.
28598407	8	45	from	products	1484:1491	arg1	polysaccharides					1453:1467	bioactive polysaccharides	1443:1467	bioactive polysaccharides from processed products of herbal medicines	1443:1511	Collectively, this study provides a promising strategy to obtain bioactive polysaccharides from processed products of herbal medicines.
28598407	2	46	theme	structural	356:365	arg1	characteristics					367:381	The structural characteristics	352:381	The structural characteristics of PSA	352:388	The structural characteristics of PSA were analyzed by UV-vis spectroscopy, infrared spectroscopy, scanning electron microscopy, high performance gel permeation chromatography and high performance liquid chromatography, respectively.
28598407	2	47	theme	high	481:484	arg1	chromatography					513:526	high performance gel permeation chromatography	481:526	high performance gel permeation chromatography	481:526	The structural characteristics of PSA were analyzed by UV-vis spectroscopy, infrared spectroscopy, scanning electron microscopy, high performance gel permeation chromatography and high performance liquid chromatography, respectively.
28598407	4	48	theme	W.	896:897	arg1	power					883:887	ultrasonic power	872:887	ultrasonic power	872:887	Results indicated that the optimal extraction conditions were as follows: the ratio of water to raw of 30 mL/g, extraction time of 46.50 min, ultrasonic temperature of 72.00 °C, and ultrasonic power of 230 W. Under these conditions, the obtained PSA yield (13.21 ± 0.37%) was closely agreed with the predicted yield by the model.
28598407	4	48	theme	W.	896:897	arg1	time					813:816	extraction time	802:816	extraction time of 46.50 min	802:829	Results indicated that the optimal extraction conditions were as follows: the ratio of water to raw of 30 mL/g, extraction time of 46.50 min, ultrasonic temperature of 72.00 °C, and ultrasonic power of 230 W. Under these conditions, the obtained PSA yield (13.21 ± 0.37%) was closely agreed with the predicted yield by the model.
28598407	4	48	theme	W.	896:897	arg1	temperature					843:853	ultrasonic temperature	832:853	ultrasonic temperature of 72.00 °C	832:865	Results indicated that the optimal extraction conditions were as follows: the ratio of water to raw of 30 mL/g, extraction time of 46.50 min, ultrasonic temperature of 72.00 °C, and ultrasonic power of 230 W. Under these conditions, the obtained PSA yield (13.21 ± 0.37%) was closely agreed with the predicted yield by the model.
28598407	4	48	theme	W.	896:897	arg1	mL/g					796:799	30 mL/g	793:799	30 mL/g	793:799	Results indicated that the optimal extraction conditions were as follows: the ratio of water to raw of 30 mL/g, extraction time of 46.50 min, ultrasonic temperature of 72.00 °C, and ultrasonic power of 230 W. Under these conditions, the obtained PSA yield (13.21 ± 0.37%) was closely agreed with the predicted yield by the model.
28598407	3	49	theme	PSA	643:645	arg1	activities					629:638	antioxidant and antimicrobial activities	599:638	antioxidant and antimicrobial activities of PSA	599:645	In addition, antioxidant and antimicrobial activities of PSA were studied by different in vitro assays.
28598407	3	50	theme	different	663:671	arg1	assays					682:687	different in vitro assays	663:687	different in vitro assays	663:687	In addition, antioxidant and antimicrobial activities of PSA were studied by different in vitro assays.
28598407	0	51	from	Alumine	119:125	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction Optimization, Characterization, and Bioactivities of Polysaccharides from Pinelliae Rhizoma Praeparatum Cum Alumine Employing Ultrasound-Assisted Extraction.
28598407	0	51	from	Alumine	119:125	arg1	Characterization					25:40	Characterization	25:40	Characterization	25:40	Extraction Optimization, Characterization, and Bioactivities of Polysaccharides from Pinelliae Rhizoma Praeparatum Cum Alumine Employing Ultrasound-Assisted Extraction.
28598407	0	51	from	Alumine	119:125	arg1	Bioactivities					47:59	Bioactivities	47:59	Bioactivities	47:59	Extraction Optimization, Characterization, and Bioactivities of Polysaccharides from Pinelliae Rhizoma Praeparatum Cum Alumine Employing Ultrasound-Assisted Extraction.
28598407	3	52	theme	in	673:674	arg1	assays					682:687	different in vitro assays	663:687	different in vitro assays	663:687	In addition, antioxidant and antimicrobial activities of PSA were studied by different in vitro assays.
28598407	6	53	theme	composition	1127:1137	arg1	analysis					1139:1146	Monosaccharide composition analysis	1112:1146	Monosaccharide composition analysis	1112:1146	Monosaccharide composition analysis indicated that PSA consisted of mannose, galactose uronic acid, glucose, galactose, arabinose with a molar ratio of 1.83:0.55:75.75:1.94:0.45.
28598407	8	54	theme	bioactive	1443:1451	arg1	polysaccharides					1453:1467	bioactive polysaccharides	1443:1467	bioactive polysaccharides from processed products of herbal medicines	1443:1511	Collectively, this study provides a promising strategy to obtain bioactive polysaccharides from processed products of herbal medicines.
28598407	2	55	theme	performance	537:547	arg1	chromatography					556:569	high performance liquid chromatography	532:569	high performance liquid chromatography	532:569	The structural characteristics of PSA were analyzed by UV-vis spectroscopy, infrared spectroscopy, scanning electron microscopy, high performance gel permeation chromatography and high performance liquid chromatography, respectively.
28598407	5	56	theme	average	1024:1030	arg1	weights					1042:1048	The average molecular weights	1020:1048	The average molecular weights of the PSA	1020:1059	The average molecular weights of the PSA were estimated to be 5.34 × 10³ and 6.27 × 10⁵ Da.
28598407	5	56	theme	average	1024:1030	arg1	Da					1108:1109	5.34 × 10³ and 6.27 × 10⁵ Da	1082:1109	5.34 × 10³ and 6.27 × 10⁵ Da	1082:1109	The average molecular weights of the PSA were estimated to be 5.34 × 10³ and 6.27 × 10⁵ Da.
28598407	4	57	theme	min	827:829	arg1	power					883:887	ultrasonic power	872:887	ultrasonic power	872:887	Results indicated that the optimal extraction conditions were as follows: the ratio of water to raw of 30 mL/g, extraction time of 46.50 min, ultrasonic temperature of 72.00 °C, and ultrasonic power of 230 W. Under these conditions, the obtained PSA yield (13.21 ± 0.37%) was closely agreed with the predicted yield by the model.
28598407	4	57	theme	min	827:829	arg1	time					813:816	extraction time	802:816	extraction time of 46.50 min	802:829	Results indicated that the optimal extraction conditions were as follows: the ratio of water to raw of 30 mL/g, extraction time of 46.50 min, ultrasonic temperature of 72.00 °C, and ultrasonic power of 230 W. Under these conditions, the obtained PSA yield (13.21 ± 0.37%) was closely agreed with the predicted yield by the model.
28598407	4	57	theme	min	827:829	arg1	temperature					843:853	ultrasonic temperature	832:853	ultrasonic temperature of 72.00 °C	832:865	Results indicated that the optimal extraction conditions were as follows: the ratio of water to raw of 30 mL/g, extraction time of 46.50 min, ultrasonic temperature of 72.00 °C, and ultrasonic power of 230 W. Under these conditions, the obtained PSA yield (13.21 ± 0.37%) was closely agreed with the predicted yield by the model.
28598407	4	57	theme	min	827:829	arg1	mL/g					796:799	30 mL/g	793:799	30 mL/g	793:799	Results indicated that the optimal extraction conditions were as follows: the ratio of water to raw of 30 mL/g, extraction time of 46.50 min, ultrasonic temperature of 72.00 °C, and ultrasonic power of 230 W. Under these conditions, the obtained PSA yield (13.21 ± 0.37%) was closely agreed with the predicted yield by the model.
28598407	2	58	theme	high	532:535	arg1	chromatography					556:569	high performance liquid chromatography	532:569	high performance liquid chromatography	532:569	The structural characteristics of PSA were analyzed by UV-vis spectroscopy, infrared spectroscopy, scanning electron microscopy, high performance gel permeation chromatography and high performance liquid chromatography, respectively.
28598407	8	59	theme	processed	1474:1482	arg1	products					1484:1491	processed products	1474:1491	processed products of herbal medicines	1474:1511	Collectively, this study provides a promising strategy to obtain bioactive polysaccharides from processed products of herbal medicines.
28598407	0	60	dep	Extraction	0:9	arg1	Optimization					11:22	Optimization	11:22	Optimization	11:22	Extraction Optimization, Characterization, and Bioactivities of Polysaccharides from Pinelliae Rhizoma Praeparatum Cum Alumine Employing Ultrasound-Assisted Extraction.
28598407	2	61	theme	PSA	386:388	arg1	characteristics					367:381	The structural characteristics	352:381	The structural characteristics of PSA	352:388	The structural characteristics of PSA were analyzed by UV-vis spectroscopy, infrared spectroscopy, scanning electron microscopy, high performance gel permeation chromatography and high performance liquid chromatography, respectively.
28598407	6	62	with	mannose	1180:1186	arg1	ratio					1255:1259	a molar ratio	1247:1259	a molar ratio of 1.83:0.55:75.75:1.94:0.45	1247:1288	Monosaccharide composition analysis indicated that PSA consisted of mannose, galactose uronic acid, glucose, galactose, arabinose with a molar ratio of 1.83:0.55:75.75:1.94:0.45.
29352906	0	0	theme	wound	80:84	arg1	healing					86:92	wound healing	80:92	wound healing	80:92	Preparation of composite hydroxybutyl chitosan sponge and its role in promoting wound healing.
29352906	8	1	theme	wound	1546:1550	arg1	dressings					1552:1560	wound dressings	1546:1560	wound dressings	1546:1560	The obtained data encourage the use of this composite sponge for wound dressings.
29352906	8	2	theme	obtained	1485:1492	arg1	data					1494:1497	The obtained data	1481:1497	The obtained data	1481:1497	The obtained data encourage the use of this composite sponge for wound dressings.
29352906	2	3	theme	chitosan	367:374	arg1	incorporation					350:362	the incorporation	346:362	the incorporation of chitosan into hydroxybutyl chitosan	346:401	Hydrophilic and macroporous composite hydroxybutyl chitosan sponge was developed via the incorporation of chitosan into hydroxybutyl chitosan.
29352906	7	4	theme	epithelial	1205:1214	arg1	cells					1216:1220	epithelial cells	1205:1220	epithelial cells attached to the composite sponge and penetrated into the interior	1205:1286	Eventually, the vivo evaluations in Sprague-Dawley rats revealed that epithelial cells attached to the composite sponge and penetrated into the interior, in addition to this, it was also proved that the composite sponge (HC-1) had a better ability to promote wound healing and helped for faster formation of skin glands and re-epithelialization.
29352906	5	5	theme	composite	892:900	arg1	sponge					902:907	composite sponge	892:907	composite sponge	892:907	Cytocompatibility tests with L929 cells and HUVEC cells demonstrated that composite sponge were no cytotoxicity, and could promote the growth of fibroblasts.
29352906	1	6	theme	vacuum	239:244	arg1	freeze-drying					246:258	vacuum freeze-drying	239:258	vacuum freeze-drying	239:258	In this work, a composite sponge was produced by physically mixing hydroxybutyl chitosan with chitosan to form a porous spongy material through vacuum freeze-drying.
29352906	6	7	theme	higher	1084:1089	arg1	level					1091:1095	a higher level	1082:1095	a higher level of antibacterial (>99.99% reduction)	1082:1132	It made up for the shortcomings of hydroxybutyl chitosan with unfavorable antibacterial effect to achieve a higher level of antibacterial (>99.99% reduction).
29352906	7	8	theme	faster	1423:1428	arg1	formation					1430:1438	faster formation	1423:1438	faster formation of skin glands and re-epithelialization	1423:1478	Eventually, the vivo evaluations in Sprague-Dawley rats revealed that epithelial cells attached to the composite sponge and penetrated into the interior, in addition to this, it was also proved that the composite sponge (HC-1) had a better ability to promote wound healing and helped for faster formation of skin glands and re-epithelialization.
29352906	2	9	theme	composite	289:297	arg1	sponge					321:326	Hydrophilic and macroporous composite hydroxybutyl chitosan sponge	261:326	Hydrophilic and macroporous composite hydroxybutyl chitosan sponge	261:326	Hydrophilic and macroporous composite hydroxybutyl chitosan sponge was developed via the incorporation of chitosan into hydroxybutyl chitosan.
29352906	3	10	theme	blood-clotting	530:543	arg1	BCI					552:554	BCI	552:554	BCI	552:554	The composite sponge showed higher porosity (about 85%), greater water absorption (about 25 times), better softness and lower blood-clotting index (BCI) than those of chitosan sponge and hydroxybutyl chitosan sponge.
29352906	3	10	theme	blood-clotting	530:543	arg1	index					545:549	lower blood-clotting index	524:549	lower blood-clotting index (BCI)	524:555	The composite sponge showed higher porosity (about 85%), greater water absorption (about 25 times), better softness and lower blood-clotting index (BCI) than those of chitosan sponge and hydroxybutyl chitosan sponge.
29352906	6	11	theme	antibacterial	1100:1112	arg1	level					1091:1095	a higher level	1082:1095	a higher level of antibacterial (>99.99% reduction)	1082:1132	It made up for the shortcomings of hydroxybutyl chitosan with unfavorable antibacterial effect to achieve a higher level of antibacterial (>99.99% reduction).
29352906	7	12	theme	composite	1238:1246	arg1	sponge					1248:1253	the composite sponge	1234:1253	the composite sponge	1234:1253	Eventually, the vivo evaluations in Sprague-Dawley rats revealed that epithelial cells attached to the composite sponge and penetrated into the interior, in addition to this, it was also proved that the composite sponge (HC-1) had a better ability to promote wound healing and helped for faster formation of skin glands and re-epithelialization.
29352906	5	13	theme	Cytocompatibility	818:834	arg1	tests					836:840	Cytocompatibility tests	818:840	Cytocompatibility tests with L929 cells and HUVEC cells	818:872	Cytocompatibility tests with L929 cells and HUVEC cells demonstrated that composite sponge were no cytotoxicity, and could promote the growth of fibroblasts.
29352906	7	14	theme	better	1368:1373	arg1	ability					1375:1381	a better ability	1366:1381	a better ability to promote wound healing and helped for faster formation of skin glands and re-epithelialization	1366:1478	Eventually, the vivo evaluations in Sprague-Dawley rats revealed that epithelial cells attached to the composite sponge and penetrated into the interior, in addition to this, it was also proved that the composite sponge (HC-1) had a better ability to promote wound healing and helped for faster formation of skin glands and re-epithelialization.
29352906	5	15	theme	L929	847:850	arg1	cells					852:856	L929 cells	847:856	L929 cells	847:856	Cytocompatibility tests with L929 cells and HUVEC cells demonstrated that composite sponge were no cytotoxicity, and could promote the growth of fibroblasts.
29352906	7	16	theme	Sprague-Dawley	1171:1184	arg1	rats					1186:1189	Sprague-Dawley rats	1171:1189	Sprague-Dawley rats	1171:1189	Eventually, the vivo evaluations in Sprague-Dawley rats revealed that epithelial cells attached to the composite sponge and penetrated into the interior, in addition to this, it was also proved that the composite sponge (HC-1) had a better ability to promote wound healing and helped for faster formation of skin glands and re-epithelialization.
29352906	7	17	theme	wound	1394:1398	arg1	healing					1400:1406	wound healing	1394:1406	wound healing	1394:1406	Eventually, the vivo evaluations in Sprague-Dawley rats revealed that epithelial cells attached to the composite sponge and penetrated into the interior, in addition to this, it was also proved that the composite sponge (HC-1) had a better ability to promote wound healing and helped for faster formation of skin glands and re-epithelialization.
29352906	3	18	theme	higher	432:437	arg1	%					457:457	about 85%	449:457	about 85%	449:457	The composite sponge showed higher porosity (about 85%), greater water absorption (about 25 times), better softness and lower blood-clotting index (BCI) than those of chitosan sponge and hydroxybutyl chitosan sponge.
29352906	3	18	theme	higher	432:437	arg1	porosity					439:446	higher porosity	432:446	higher porosity (about 85%)	432:458	The composite sponge showed higher porosity (about 85%), greater water absorption (about 25 times), better softness and lower blood-clotting index (BCI) than those of chitosan sponge and hydroxybutyl chitosan sponge.
29352906	2	19	theme	Hydrophilic	261:271	arg1	sponge					321:326	Hydrophilic and macroporous composite hydroxybutyl chitosan sponge	261:326	Hydrophilic and macroporous composite hydroxybutyl chitosan sponge	261:326	Hydrophilic and macroporous composite hydroxybutyl chitosan sponge was developed via the incorporation of chitosan into hydroxybutyl chitosan.
29352906	7	20	theme	vivo	1151:1154	arg1	evaluations					1156:1166	the vivo evaluations	1147:1166	the vivo evaluations in Sprague-Dawley rats	1147:1189	Eventually, the vivo evaluations in Sprague-Dawley rats revealed that epithelial cells attached to the composite sponge and penetrated into the interior, in addition to this, it was also proved that the composite sponge (HC-1) had a better ability to promote wound healing and helped for faster formation of skin glands and re-epithelialization.
29352906	7	21	from	evaluations	1156:1166	arg1	rats					1186:1189	Sprague-Dawley rats	1171:1189	Sprague-Dawley rats	1171:1189	Eventually, the vivo evaluations in Sprague-Dawley rats revealed that epithelial cells attached to the composite sponge and penetrated into the interior, in addition to this, it was also proved that the composite sponge (HC-1) had a better ability to promote wound healing and helped for faster formation of skin glands and re-epithelialization.
29352906	4	22	theme	blood	715:719	arg1	concentration					721:733	blood concentration	715:733	blood concentration	715:733	The composite sponge with good hydrophilic could absorb the moisture in the blood to increase blood concentration and viscosity, and become a semi-swelling viscous colloid to clog the capillaries.
29352906	1	23	theme	hydroxybutyl	162:173	arg1	chitosan					175:182	hydroxybutyl chitosan	162:182	hydroxybutyl chitosan with chitosan to form a porous spongy material through vacuum freeze-drying	162:258	In this work, a composite sponge was produced by physically mixing hydroxybutyl chitosan with chitosan to form a porous spongy material through vacuum freeze-drying.
29352906	3	24	theme	composite	408:416	arg1	sponge					418:423	The composite sponge	404:423	The composite sponge	404:423	The composite sponge showed higher porosity (about 85%), greater water absorption (about 25 times), better softness and lower blood-clotting index (BCI) than those of chitosan sponge and hydroxybutyl chitosan sponge.
29352906	5	25	theme	HUVEC	862:866	arg1	cells					868:872	HUVEC cells	862:872	HUVEC cells	862:872	Cytocompatibility tests with L929 cells and HUVEC cells demonstrated that composite sponge were no cytotoxicity, and could promote the growth of fibroblasts.
29352906	0	26	theme	hydroxybutyl	25:36	arg1	sponge					47:52	composite hydroxybutyl chitosan sponge	15:52	composite hydroxybutyl chitosan sponge	15:52	Preparation of composite hydroxybutyl chitosan sponge and its role in promoting wound healing.
29352906	5	27	with	tests	836:840	arg1	cells					852:856	L929 cells	847:856	L929 cells	847:856	Cytocompatibility tests with L929 cells and HUVEC cells demonstrated that composite sponge were no cytotoxicity, and could promote the growth of fibroblasts.
29352906	5	27	with	tests	836:840	arg1	cells					868:872	HUVEC cells	862:872	HUVEC cells	862:872	Cytocompatibility tests with L929 cells and HUVEC cells demonstrated that composite sponge were no cytotoxicity, and could promote the growth of fibroblasts.
29352906	7	28	theme	composite	1338:1346	arg1	HC-1					1356:1359	HC-1	1356:1359	HC-1	1356:1359	Eventually, the vivo evaluations in Sprague-Dawley rats revealed that epithelial cells attached to the composite sponge and penetrated into the interior, in addition to this, it was also proved that the composite sponge (HC-1) had a better ability to promote wound healing and helped for faster formation of skin glands and re-epithelialization.
29352906	7	28	theme	composite	1338:1346	arg1	sponge					1348:1353	the composite sponge	1334:1353	the composite sponge (HC-1)	1334:1360	Eventually, the vivo evaluations in Sprague-Dawley rats revealed that epithelial cells attached to the composite sponge and penetrated into the interior, in addition to this, it was also proved that the composite sponge (HC-1) had a better ability to promote wound healing and helped for faster formation of skin glands and re-epithelialization.
29352906	2	29	theme	chitosan	312:319	arg1	sponge					321:326	Hydrophilic and macroporous composite hydroxybutyl chitosan sponge	261:326	Hydrophilic and macroporous composite hydroxybutyl chitosan sponge	261:326	Hydrophilic and macroporous composite hydroxybutyl chitosan sponge was developed via the incorporation of chitosan into hydroxybutyl chitosan.
29352906	3	30	theme	greater	461:467	arg1	absorption					475:484	greater water absorption	461:484	greater water absorption (about 25 times)	461:501	The composite sponge showed higher porosity (about 85%), greater water absorption (about 25 times), better softness and lower blood-clotting index (BCI) than those of chitosan sponge and hydroxybutyl chitosan sponge.
29352906	3	30	theme	greater	461:467	arg1	times					496:500	about 25 times	487:500	about 25 times	487:500	The composite sponge showed higher porosity (about 85%), greater water absorption (about 25 times), better softness and lower blood-clotting index (BCI) than those of chitosan sponge and hydroxybutyl chitosan sponge.
29352906	0	31	theme	composite	15:23	arg1	sponge					47:52	composite hydroxybutyl chitosan sponge	15:52	composite hydroxybutyl chitosan sponge	15:52	Preparation of composite hydroxybutyl chitosan sponge and its role in promoting wound healing.
29352906	2	32	theme	hydroxybutyl	299:310	arg1	sponge					321:326	Hydrophilic and macroporous composite hydroxybutyl chitosan sponge	261:326	Hydrophilic and macroporous composite hydroxybutyl chitosan sponge	261:326	Hydrophilic and macroporous composite hydroxybutyl chitosan sponge was developed via the incorporation of chitosan into hydroxybutyl chitosan.
29352906	3	33	theme	water	469:473	arg1	absorption					475:484	greater water absorption	461:484	greater water absorption (about 25 times)	461:501	The composite sponge showed higher porosity (about 85%), greater water absorption (about 25 times), better softness and lower blood-clotting index (BCI) than those of chitosan sponge and hydroxybutyl chitosan sponge.
29352906	3	33	theme	water	469:473	arg1	times					496:500	about 25 times	487:500	about 25 times	487:500	The composite sponge showed higher porosity (about 85%), greater water absorption (about 25 times), better softness and lower blood-clotting index (BCI) than those of chitosan sponge and hydroxybutyl chitosan sponge.
29352906	0	34	theme	sponge	47:52	arg1	Preparation					0:10	Preparation	0:10	Preparation of composite hydroxybutyl chitosan sponge	0:52	Preparation of composite hydroxybutyl chitosan sponge and its role in promoting wound healing.
29352906	0	34	theme	sponge	47:52	arg1	role					62:65	its role	58:65	its role in promoting wound healing	58:92	Preparation of composite hydroxybutyl chitosan sponge and its role in promoting wound healing.
29352906	7	35	contain	had	1362:1364	arg2	ability					1375:1381	a better ability	1366:1381	a better ability to promote wound healing and helped for faster formation of skin glands and re-epithelialization	1366:1478	Eventually, the vivo evaluations in Sprague-Dawley rats revealed that epithelial cells attached to the composite sponge and penetrated into the interior, in addition to this, it was also proved that the composite sponge (HC-1) had a better ability to promote wound healing and helped for faster formation of skin glands and re-epithelialization.
29352906	7	35	contain	had	1362:1364	arg1	sponge					1348:1353	the composite sponge	1334:1353	the composite sponge (HC-1)	1334:1360	Eventually, the vivo evaluations in Sprague-Dawley rats revealed that epithelial cells attached to the composite sponge and penetrated into the interior, in addition to this, it was also proved that the composite sponge (HC-1) had a better ability to promote wound healing and helped for faster formation of skin glands and re-epithelialization.
29352906	7	35	contain	had	1362:1364	arg1	HC-1					1356:1359	HC-1	1356:1359	HC-1	1356:1359	Eventually, the vivo evaluations in Sprague-Dawley rats revealed that epithelial cells attached to the composite sponge and penetrated into the interior, in addition to this, it was also proved that the composite sponge (HC-1) had a better ability to promote wound healing and helped for faster formation of skin glands and re-epithelialization.
29352906	7	36	dep	cells	1216:1220	arg1	penetrated					1259:1268	penetrated	1259:1268	penetrated into the interior	1259:1286	Eventually, the vivo evaluations in Sprague-Dawley rats revealed that epithelial cells attached to the composite sponge and penetrated into the interior, in addition to this, it was also proved that the composite sponge (HC-1) had a better ability to promote wound healing and helped for faster formation of skin glands and re-epithelialization.
29352906	7	36	dep	cells	1216:1220	arg1	attached					1222:1229	attached	1222:1229	attached to the composite sponge	1222:1253	Eventually, the vivo evaluations in Sprague-Dawley rats revealed that epithelial cells attached to the composite sponge and penetrated into the interior, in addition to this, it was also proved that the composite sponge (HC-1) had a better ability to promote wound healing and helped for faster formation of skin glands and re-epithelialization.
29352906	6	37	theme	chitosan	1024:1031	arg1	shortcomings					995:1006	the shortcomings	991:1006	the shortcomings of hydroxybutyl chitosan with unfavorable antibacterial effect to achieve a higher level of antibacterial (>99.99% reduction)	991:1132	It made up for the shortcomings of hydroxybutyl chitosan with unfavorable antibacterial effect to achieve a higher level of antibacterial (>99.99% reduction).
29352906	6	38	theme	antibacterial	1050:1062	arg1	effect					1064:1069	unfavorable antibacterial effect	1038:1069	unfavorable antibacterial effect to achieve a higher level of antibacterial (>99.99% reduction)	1038:1132	It made up for the shortcomings of hydroxybutyl chitosan with unfavorable antibacterial effect to achieve a higher level of antibacterial (>99.99% reduction).
29352906	4	39	theme	composite	625:633	arg1	sponge					635:640	The composite sponge	621:640	The composite sponge with good hydrophilic	621:662	The composite sponge with good hydrophilic could absorb the moisture in the blood to increase blood concentration and viscosity, and become a semi-swelling viscous colloid to clog the capillaries.
29352906	0	40	theme	chitosan	38:45	arg1	sponge					47:52	composite hydroxybutyl chitosan sponge	15:52	composite hydroxybutyl chitosan sponge	15:52	Preparation of composite hydroxybutyl chitosan sponge and its role in promoting wound healing.
29352906	8	41	theme	composite	1525:1533	arg1	sponge					1535:1540	this composite sponge	1520:1540	this composite sponge	1520:1540	The obtained data encourage the use of this composite sponge for wound dressings.
29352906	3	42	theme	chitosan	571:578	arg1	sponge					580:585	chitosan sponge	571:585	chitosan sponge	571:585	The composite sponge showed higher porosity (about 85%), greater water absorption (about 25 times), better softness and lower blood-clotting index (BCI) than those of chitosan sponge and hydroxybutyl chitosan sponge.
29352906	6	43	theme	hydroxybutyl	1011:1022	arg1	chitosan					1024:1031	hydroxybutyl chitosan	1011:1031	hydroxybutyl chitosan with unfavorable antibacterial effect to achieve a higher level of antibacterial (>99.99% reduction)	1011:1132	It made up for the shortcomings of hydroxybutyl chitosan with unfavorable antibacterial effect to achieve a higher level of antibacterial (>99.99% reduction).
29352906	4	44	with	sponge	635:640	arg1	hydrophilic					652:662	hydrophilic	652:662	hydrophilic	652:662	The composite sponge with good hydrophilic could absorb the moisture in the blood to increase blood concentration and viscosity, and become a semi-swelling viscous colloid to clog the capillaries.
29352906	6	45	theme	%	1121:1121	arg1	reduction					1123:1131	>99.99% reduction	1115:1131	>99.99% reduction	1115:1131	It made up for the shortcomings of hydroxybutyl chitosan with unfavorable antibacterial effect to achieve a higher level of antibacterial (>99.99% reduction).
29352906	6	45	theme	%	1121:1121	arg1	antibacterial					1100:1112	antibacterial	1100:1112	antibacterial (>99.99% reduction)	1100:1132	It made up for the shortcomings of hydroxybutyl chitosan with unfavorable antibacterial effect to achieve a higher level of antibacterial (>99.99% reduction).
29352906	1	46	with	chitosan	175:182	arg1	chitosan					189:196	chitosan	189:196	chitosan	189:196	In this work, a composite sponge was produced by physically mixing hydroxybutyl chitosan with chitosan to form a porous spongy material through vacuum freeze-drying.
29352906	6	47	with	chitosan	1024:1031	arg1	effect					1064:1069	unfavorable antibacterial effect	1038:1069	unfavorable antibacterial effect to achieve a higher level of antibacterial (>99.99% reduction)	1038:1132	It made up for the shortcomings of hydroxybutyl chitosan with unfavorable antibacterial effect to achieve a higher level of antibacterial (>99.99% reduction).
29352906	7	48	theme	re-epithelialization	1459:1478	arg1	formation					1430:1438	faster formation	1423:1438	faster formation of skin glands and re-epithelialization	1423:1478	Eventually, the vivo evaluations in Sprague-Dawley rats revealed that epithelial cells attached to the composite sponge and penetrated into the interior, in addition to this, it was also proved that the composite sponge (HC-1) had a better ability to promote wound healing and helped for faster formation of skin glands and re-epithelialization.
29352906	6	49	theme	>99.99	1115:1120	arg1	%					1121:1121	%	1121:1121	%	1121:1121	It made up for the shortcomings of hydroxybutyl chitosan with unfavorable antibacterial effect to achieve a higher level of antibacterial (>99.99% reduction).
29352906	8	50	theme	sponge	1535:1540	arg1	use					1513:1515	the use	1509:1515	the use of this composite sponge for wound dressings	1509:1560	The obtained data encourage the use of this composite sponge for wound dressings.
29352906	4	51	theme	semi-swelling	763:775	arg1	colloid					785:791	a semi-swelling viscous colloid	761:791	a semi-swelling viscous colloid to clog the capillaries	761:815	The composite sponge with good hydrophilic could absorb the moisture in the blood to increase blood concentration and viscosity, and become a semi-swelling viscous colloid to clog the capillaries.
29352906	3	52	theme	hydroxybutyl	591:602	arg1	sponge					613:618	hydroxybutyl chitosan sponge	591:618	hydroxybutyl chitosan sponge	591:618	The composite sponge showed higher porosity (about 85%), greater water absorption (about 25 times), better softness and lower blood-clotting index (BCI) than those of chitosan sponge and hydroxybutyl chitosan sponge.
29352906	5	53	theme	fibroblasts	963:973	arg1	growth					953:958	the growth	949:958	the growth of fibroblasts	949:973	Cytocompatibility tests with L929 cells and HUVEC cells demonstrated that composite sponge were no cytotoxicity, and could promote the growth of fibroblasts.
29352906	7	54	theme	skin	1443:1446	arg1	glands					1448:1453	skin glands	1443:1453	skin glands	1443:1453	Eventually, the vivo evaluations in Sprague-Dawley rats revealed that epithelial cells attached to the composite sponge and penetrated into the interior, in addition to this, it was also proved that the composite sponge (HC-1) had a better ability to promote wound healing and helped for faster formation of skin glands and re-epithelialization.
29352906	3	55	theme	chitosan	604:611	arg1	sponge					613:618	hydroxybutyl chitosan sponge	591:618	hydroxybutyl chitosan sponge	591:618	The composite sponge showed higher porosity (about 85%), greater water absorption (about 25 times), better softness and lower blood-clotting index (BCI) than those of chitosan sponge and hydroxybutyl chitosan sponge.
29352906	1	56	theme	composite	111:119	arg1	sponge					121:126	a composite sponge	109:126	a composite sponge	109:126	In this work, a composite sponge was produced by physically mixing hydroxybutyl chitosan with chitosan to form a porous spongy material through vacuum freeze-drying.
29352906	1	57	theme	porous	208:213	arg1	material					222:229	a porous spongy material	206:229	a porous spongy material	206:229	In this work, a composite sponge was produced by physically mixing hydroxybutyl chitosan with chitosan to form a porous spongy material through vacuum freeze-drying.
29352906	7	58	theme	glands	1448:1453	arg1	formation					1430:1438	faster formation	1423:1438	faster formation of skin glands and re-epithelialization	1423:1478	Eventually, the vivo evaluations in Sprague-Dawley rats revealed that epithelial cells attached to the composite sponge and penetrated into the interior, in addition to this, it was also proved that the composite sponge (HC-1) had a better ability to promote wound healing and helped for faster formation of skin glands and re-epithelialization.
29352906	6	59	theme	unfavorable	1038:1048	arg1	effect					1064:1069	unfavorable antibacterial effect	1038:1069	unfavorable antibacterial effect to achieve a higher level of antibacterial (>99.99% reduction)	1038:1132	It made up for the shortcomings of hydroxybutyl chitosan with unfavorable antibacterial effect to achieve a higher level of antibacterial (>99.99% reduction).
29352906	2	60	theme	hydroxybutyl	381:392	arg1	chitosan					394:401	hydroxybutyl chitosan	381:401	hydroxybutyl chitosan	381:401	Hydrophilic and macroporous composite hydroxybutyl chitosan sponge was developed via the incorporation of chitosan into hydroxybutyl chitosan.
29352906	1	61	theme	spongy	215:220	arg1	material					222:229	a porous spongy material	206:229	a porous spongy material	206:229	In this work, a composite sponge was produced by physically mixing hydroxybutyl chitosan with chitosan to form a porous spongy material through vacuum freeze-drying.
29352906	3	62	theme	lower	524:528	arg1	BCI					552:554	BCI	552:554	BCI	552:554	The composite sponge showed higher porosity (about 85%), greater water absorption (about 25 times), better softness and lower blood-clotting index (BCI) than those of chitosan sponge and hydroxybutyl chitosan sponge.
29352906	3	62	theme	lower	524:528	arg1	index					545:549	lower blood-clotting index	524:549	lower blood-clotting index (BCI)	524:555	The composite sponge showed higher porosity (about 85%), greater water absorption (about 25 times), better softness and lower blood-clotting index (BCI) than those of chitosan sponge and hydroxybutyl chitosan sponge.
29352906	4	63	theme	viscous	777:783	arg1	colloid					785:791	a semi-swelling viscous colloid	761:791	a semi-swelling viscous colloid to clog the capillaries	761:815	The composite sponge with good hydrophilic could absorb the moisture in the blood to increase blood concentration and viscosity, and become a semi-swelling viscous colloid to clog the capillaries.
29352906	3	64	theme	better	504:509	arg1	softness					511:518	better softness	504:518	better softness	504:518	The composite sponge showed higher porosity (about 85%), greater water absorption (about 25 times), better softness and lower blood-clotting index (BCI) than those of chitosan sponge and hydroxybutyl chitosan sponge.
29352906	4	65	from	moisture	681:688	arg1	blood					697:701	the blood	693:701	the blood	693:701	The composite sponge with good hydrophilic could absorb the moisture in the blood to increase blood concentration and viscosity, and become a semi-swelling viscous colloid to clog the capillaries.
29352906	7	66	dep	ability	1375:1381	arg1	helped					1412:1417	helped	1412:1417	helped for faster formation of skin glands and re-epithelialization	1412:1478	Eventually, the vivo evaluations in Sprague-Dawley rats revealed that epithelial cells attached to the composite sponge and penetrated into the interior, in addition to this, it was also proved that the composite sponge (HC-1) had a better ability to promote wound healing and helped for faster formation of skin glands and re-epithelialization.
29352906	7	66	dep	ability	1375:1381	arg1	promote					1386:1392	promote	1386:1392	to promote wound healing	1383:1406	Eventually, the vivo evaluations in Sprague-Dawley rats revealed that epithelial cells attached to the composite sponge and penetrated into the interior, in addition to this, it was also proved that the composite sponge (HC-1) had a better ability to promote wound healing and helped for faster formation of skin glands and re-epithelialization.
25965462	6	0	theme	control	1097:1103	arg1	film					1109:1112	the control CMC film	1093:1112	the control CMC film	1093:1112	The WVP of CMC film decreased at low content of CNF, and increased with increase in CNF content, then decreased but to the same level of the control CMC film with the inclusion of 10 wt% of CNF.
25965462	5	1	theme	inclusion	945:953	arg1	%					936:936	5 wt%	932:936	5 wt% of CNF inclusion	932:953	The tensile strength (TS) and elastic modulus (EM) of CMC film increased by 23% and 27%, respectively, while the elongation (E) decreased by 28% with 5 wt% of CNF inclusion.
25965462	5	1	theme	inclusion	945:953	arg1	inclusion					945:953	CNF inclusion	941:953	CNF inclusion	941:953	The tensile strength (TS) and elastic modulus (EM) of CMC film increased by 23% and 27%, respectively, while the elongation (E) decreased by 28% with 5 wt% of CNF inclusion.
25965462	7	2	theme	CMC	1167:1169	arg1	film					1171:1174	CMC film	1167:1174	CMC film	1167:1174	Transparency of CMC film decreased slightly from 87.7% to 86.2% with 5 wt% of CNF.
25965462	1	3	theme	cotton	176:181	arg1	pulp					190:193	cotton linter pulp	176:193	cotton linter pulp	176:193	Crystalline cellulose nanofibril (CNF) was isolated from cotton linter pulp using an acid hydrolysis method and used as a filler to reinforce sodium carboxymethyl cellulose (CMC) film.
25965462	8	4	theme	high	1269:1272	arg1	potential					1274:1282	a high potential	1267:1282	a high potential to be used as an edible coating or packaging films for the extension of shelf life of fresh and minimally processed fruits and vegetables	1267:1420	The CMC/CNF composite films have a high potential to be used as an edible coating or packaging films for the extension of shelf life of fresh and minimally processed fruits and vegetables.
25965462	0	5	theme	composite	102:110	arg1	films					112:116	cellulose nanofibril composite films	81:116	cellulose nanofibril composite films	81:116	Preparation and characterization of sodium carboxymethyl cellulose/cotton linter cellulose nanofibril composite films.
25965462	3	6	theme	surface	558:564	arg1	hydrophobicity					566:579	surface hydrophobicity	558:579	surface hydrophobicity	558:579	The effect of CNF concentration (1, 3, 5, and 10 wt% based on CMC) on the optical, morphological, mechanical, water vapor barrier, surface hydrophobicity, and thermal properties of the nanocomposites were studied.
25965462	1	7	theme	linter	183:188	arg1	pulp					190:193	cotton linter pulp	176:193	cotton linter pulp	176:193	Crystalline cellulose nanofibril (CNF) was isolated from cotton linter pulp using an acid hydrolysis method and used as a filler to reinforce sodium carboxymethyl cellulose (CMC) film.
25965462	1	8	theme	sodium	261:266	arg1	film					298:301	sodium carboxymethyl cellulose (CMC) film	261:301	sodium carboxymethyl cellulose (CMC) film	261:301	Crystalline cellulose nanofibril (CNF) was isolated from cotton linter pulp using an acid hydrolysis method and used as a filler to reinforce sodium carboxymethyl cellulose (CMC) film.
25965462	7	9	theme	film	1171:1174	arg1	Transparency					1151:1162	Transparency	1151:1162	Transparency of CMC film	1151:1174	Transparency of CMC film decreased slightly from 87.7% to 86.2% with 5 wt% of CNF.
25965462	5	10	theme	CNF	941:943	arg1	inclusion					945:953	CNF inclusion	941:953	CNF inclusion	941:953	The tensile strength (TS) and elastic modulus (EM) of CMC film increased by 23% and 27%, respectively, while the elongation (E) decreased by 28% with 5 wt% of CNF inclusion.
25965462	5	11	theme	elastic	812:818	arg1	modulus					820:826	elastic modulus	812:826	elastic modulus (EM) of CMC film	812:843	The tensile strength (TS) and elastic modulus (EM) of CMC film increased by 23% and 27%, respectively, while the elongation (E) decreased by 28% with 5 wt% of CNF inclusion.
25965462	5	11	theme	elastic	812:818	arg1	EM					829:830	EM	829:830	EM	829:830	The tensile strength (TS) and elastic modulus (EM) of CMC film increased by 23% and 27%, respectively, while the elongation (E) decreased by 28% with 5 wt% of CNF inclusion.
25965462	6	12	theme	CNF	1146:1148	arg1	CNF					1146:1148	CNF	1146:1148	CNF	1146:1148	The WVP of CMC film decreased at low content of CNF, and increased with increase in CNF content, then decreased but to the same level of the control CMC film with the inclusion of 10 wt% of CNF.
25965462	6	12	theme	CNF	1146:1148	arg1	%					1141:1141	10 wt%	1136:1141	10 wt% of CNF	1136:1148	The WVP of CMC film decreased at low content of CNF, and increased with increase in CNF content, then decreased but to the same level of the control CMC film with the inclusion of 10 wt% of CNF.
25965462	1	13	theme	carboxymethyl	268:280	arg1	film					298:301	sodium carboxymethyl cellulose (CMC) film	261:301	sodium carboxymethyl cellulose (CMC) film	261:301	Crystalline cellulose nanofibril (CNF) was isolated from cotton linter pulp using an acid hydrolysis method and used as a filler to reinforce sodium carboxymethyl cellulose (CMC) film.
25965462	3	14	dep	concentration	445:457	arg1	%					478:478	1, 3, 5, and 10 wt%	460:478	1, 3, 5, and 10 wt% based on CMC	460:491	The effect of CNF concentration (1, 3, 5, and 10 wt% based on CMC) on the optical, morphological, mechanical, water vapor barrier, surface hydrophobicity, and thermal properties of the nanocomposites were studied.
25965462	3	15	theme	concentration	445:457	arg1	effect					431:436	The effect	427:436	The effect of CNF concentration (1, 3, 5, and 10 wt% based on CMC) on the optical, morphological, mechanical, water vapor barrier, surface hydrophobicity, and thermal properties of the nanocomposites	427:625	The effect of CNF concentration (1, 3, 5, and 10 wt% based on CMC) on the optical, morphological, mechanical, water vapor barrier, surface hydrophobicity, and thermal properties of the nanocomposites were studied.
25965462	1	16	theme	cellulose	282:290	arg1	film					298:301	sodium carboxymethyl cellulose (CMC) film	261:301	sodium carboxymethyl cellulose (CMC) film	261:301	Crystalline cellulose nanofibril (CNF) was isolated from cotton linter pulp using an acid hydrolysis method and used as a filler to reinforce sodium carboxymethyl cellulose (CMC) film.
25965462	6	17	theme	same	1079:1082	arg1	level					1084:1088	the same level	1075:1088	the same level of the control CMC film	1075:1112	The WVP of CMC film decreased at low content of CNF, and increased with increase in CNF content, then decreased but to the same level of the control CMC film with the inclusion of 10 wt% of CNF.
25965462	1	18	attach	isolated	162:169	arg2	nanofibril					141:150	Crystalline cellulose nanofibril	119:150	Crystalline cellulose nanofibril (CNF)	119:156	Crystalline cellulose nanofibril (CNF) was isolated from cotton linter pulp using an acid hydrolysis method and used as a filler to reinforce sodium carboxymethyl cellulose (CMC) film.
25965462	1	18	attach	isolated	162:169	arg1	pulp					190:193	cotton linter pulp	176:193	cotton linter pulp	176:193	Crystalline cellulose nanofibril (CNF) was isolated from cotton linter pulp using an acid hydrolysis method and used as a filler to reinforce sodium carboxymethyl cellulose (CMC) film.
25965462	1	18	attach	isolated	162:169	arg2	CNF					153:155	CNF	153:155	CNF	153:155	Crystalline cellulose nanofibril (CNF) was isolated from cotton linter pulp using an acid hydrolysis method and used as a filler to reinforce sodium carboxymethyl cellulose (CMC) film.
25965462	1	18	attach	isolated	162:169	arg2	filler					241:246	a filler	239:246	a filler	239:246	Crystalline cellulose nanofibril (CNF) was isolated from cotton linter pulp using an acid hydrolysis method and used as a filler to reinforce sodium carboxymethyl cellulose (CMC) film.
25965462	4	19	theme	flexible	713:720	arg1	films					722:726	smooth and flexible films	702:726	smooth and flexible films	702:726	The CNF was evenly distributed in the polymer matrix to form smooth and flexible films indicating the CNF is highly compatible with the CMC.
25965462	3	20	theme	thermal	586:592	arg1	properties					594:603	thermal properties	586:603	thermal properties	586:603	The effect of CNF concentration (1, 3, 5, and 10 wt% based on CMC) on the optical, morphological, mechanical, water vapor barrier, surface hydrophobicity, and thermal properties of the nanocomposites were studied.
25965462	6	21	theme	CNF	1004:1006	arg1	content					993:999	low content	989:999	low content of CNF	989:1006	The WVP of CMC film decreased at low content of CNF, and increased with increase in CNF content, then decreased but to the same level of the control CMC film with the inclusion of 10 wt% of CNF.
25965462	7	22	dep	%	1213:1213	arg1	to					1206:1207	to	1206:1207	to	1206:1207	Transparency of CMC film decreased slightly from 87.7% to 86.2% with 5 wt% of CNF.
25965462	8	23	theme	shelf	1356:1360	arg1	life					1362:1365	shelf life	1356:1365	shelf life	1356:1365	The CMC/CNF composite films have a high potential to be used as an edible coating or packaging films for the extension of shelf life of fresh and minimally processed fruits and vegetables.
25965462	8	24	theme	composite	1246:1254	arg1	films					1256:1260	The CMC/CNF composite films	1234:1260	The CMC/CNF composite films	1234:1260	The CMC/CNF composite films have a high potential to be used as an edible coating or packaging films for the extension of shelf life of fresh and minimally processed fruits and vegetables.
25965462	3	25	from	effect	431:436	arg1	properties					594:603	thermal properties	586:603	thermal properties	586:603	The effect of CNF concentration (1, 3, 5, and 10 wt% based on CMC) on the optical, morphological, mechanical, water vapor barrier, surface hydrophobicity, and thermal properties of the nanocomposites were studied.
25965462	3	25	from	effect	431:436	arg1	morphological					510:522	morphological	510:522	morphological	510:522	The effect of CNF concentration (1, 3, 5, and 10 wt% based on CMC) on the optical, morphological, mechanical, water vapor barrier, surface hydrophobicity, and thermal properties of the nanocomposites were studied.
25965462	3	25	from	effect	431:436	arg1	mechanical					525:534	mechanical	525:534	mechanical	525:534	The effect of CNF concentration (1, 3, 5, and 10 wt% based on CMC) on the optical, morphological, mechanical, water vapor barrier, surface hydrophobicity, and thermal properties of the nanocomposites were studied.
25965462	3	25	from	effect	431:436	arg1	hydrophobicity					566:579	surface hydrophobicity	558:579	surface hydrophobicity	558:579	The effect of CNF concentration (1, 3, 5, and 10 wt% based on CMC) on the optical, morphological, mechanical, water vapor barrier, surface hydrophobicity, and thermal properties of the nanocomposites were studied.
25965462	3	25	from	effect	431:436	arg1	optical					501:507	optical	501:507	optical	501:507	The effect of CNF concentration (1, 3, 5, and 10 wt% based on CMC) on the optical, morphological, mechanical, water vapor barrier, surface hydrophobicity, and thermal properties of the nanocomposites were studied.
25965462	3	25	from	effect	431:436	arg1	barrier					549:555	water vapor barrier	537:555	water vapor barrier	537:555	The effect of CNF concentration (1, 3, 5, and 10 wt% based on CMC) on the optical, morphological, mechanical, water vapor barrier, surface hydrophobicity, and thermal properties of the nanocomposites were studied.
25965462	3	26	theme	wt	476:477	arg1	%					478:478	1, 3, 5, and 10 wt%	460:478	1, 3, 5, and 10 wt% based on CMC	460:491	The effect of CNF concentration (1, 3, 5, and 10 wt% based on CMC) on the optical, morphological, mechanical, water vapor barrier, surface hydrophobicity, and thermal properties of the nanocomposites were studied.
25965462	6	27	theme	%	1141:1141	arg1	inclusion					1123:1131	the inclusion	1119:1131	the inclusion of 10 wt% of CNF	1119:1148	The WVP of CMC film decreased at low content of CNF, and increased with increase in CNF content, then decreased but to the same level of the control CMC film with the inclusion of 10 wt% of CNF.
25965462	1	28	theme	Crystalline	119:129	arg1	filler					241:246	a filler	239:246	a filler	239:246	Crystalline cellulose nanofibril (CNF) was isolated from cotton linter pulp using an acid hydrolysis method and used as a filler to reinforce sodium carboxymethyl cellulose (CMC) film.
25965462	1	28	theme	Crystalline	119:129	arg1	CNF					153:155	CNF	153:155	CNF	153:155	Crystalline cellulose nanofibril (CNF) was isolated from cotton linter pulp using an acid hydrolysis method and used as a filler to reinforce sodium carboxymethyl cellulose (CMC) film.
25965462	1	28	theme	Crystalline	119:129	arg1	nanofibril					141:150	Crystalline cellulose nanofibril	119:150	Crystalline cellulose nanofibril (CNF)	119:156	Crystalline cellulose nanofibril (CNF) was isolated from cotton linter pulp using an acid hydrolysis method and used as a filler to reinforce sodium carboxymethyl cellulose (CMC) film.
25965462	1	29	theme	acid	204:207	arg1	method					220:225	an acid hydrolysis method	201:225	an acid hydrolysis method	201:225	Crystalline cellulose nanofibril (CNF) was isolated from cotton linter pulp using an acid hydrolysis method and used as a filler to reinforce sodium carboxymethyl cellulose (CMC) film.
25965462	2	30	theme	index	406:410	arg1	length					367:372	the length	363:372	the length of 125-217 nm and crystallinity index (CI)	363:415	The CNF was in rod shape with the diameter of 23-38 nm and the length of 125-217 nm and crystallinity index (CI) was 0.89.
25965462	2	30	theme	index	406:410	arg1	0.89					421:424	0.89	421:424	0.89	421:424	The CNF was in rod shape with the diameter of 23-38 nm and the length of 125-217 nm and crystallinity index (CI) was 0.89.
25965462	1	31	theme	CMC	293:295	arg1	film					298:301	sodium carboxymethyl cellulose (CMC) film	261:301	sodium carboxymethyl cellulose (CMC) film	261:301	Crystalline cellulose nanofibril (CNF) was isolated from cotton linter pulp using an acid hydrolysis method and used as a filler to reinforce sodium carboxymethyl cellulose (CMC) film.
25965462	5	32	theme	CMC	836:838	arg1	film					840:843	CMC film	836:843	CMC film	836:843	The tensile strength (TS) and elastic modulus (EM) of CMC film increased by 23% and 27%, respectively, while the elongation (E) decreased by 28% with 5 wt% of CNF inclusion.
25965462	8	33	contain	have	1262:1265	arg1	films					1256:1260	The CMC/CNF composite films	1234:1260	The CMC/CNF composite films	1234:1260	The CMC/CNF composite films have a high potential to be used as an edible coating or packaging films for the extension of shelf life of fresh and minimally processed fruits and vegetables.
25965462	8	33	contain	have	1262:1265	arg2	potential					1274:1282	a high potential	1267:1282	a high potential to be used as an edible coating or packaging films for the extension of shelf life of fresh and minimally processed fruits and vegetables	1267:1420	The CMC/CNF composite films have a high potential to be used as an edible coating or packaging films for the extension of shelf life of fresh and minimally processed fruits and vegetables.
25965462	1	34	theme	cellulose	131:139	arg1	filler					241:246	a filler	239:246	a filler	239:246	Crystalline cellulose nanofibril (CNF) was isolated from cotton linter pulp using an acid hydrolysis method and used as a filler to reinforce sodium carboxymethyl cellulose (CMC) film.
25965462	1	34	theme	cellulose	131:139	arg1	CNF					153:155	CNF	153:155	CNF	153:155	Crystalline cellulose nanofibril (CNF) was isolated from cotton linter pulp using an acid hydrolysis method and used as a filler to reinforce sodium carboxymethyl cellulose (CMC) film.
25965462	1	34	theme	cellulose	131:139	arg1	nanofibril					141:150	Crystalline cellulose nanofibril	119:150	Crystalline cellulose nanofibril (CNF)	119:156	Crystalline cellulose nanofibril (CNF) was isolated from cotton linter pulp using an acid hydrolysis method and used as a filler to reinforce sodium carboxymethyl cellulose (CMC) film.
25965462	6	35	theme	low	989:991	arg1	content					993:999	low content	989:999	low content of CNF	989:1006	The WVP of CMC film decreased at low content of CNF, and increased with increase in CNF content, then decreased but to the same level of the control CMC film with the inclusion of 10 wt% of CNF.
25965462	1	36	theme	hydrolysis	209:218	arg1	method					220:225	an acid hydrolysis method	201:225	an acid hydrolysis method	201:225	Crystalline cellulose nanofibril (CNF) was isolated from cotton linter pulp using an acid hydrolysis method and used as a filler to reinforce sodium carboxymethyl cellulose (CMC) film.
25965462	2	37	theme	crystallinity	392:404	arg1	CI					413:414	CI	413:414	CI	413:414	The CNF was in rod shape with the diameter of 23-38 nm and the length of 125-217 nm and crystallinity index (CI) was 0.89.
25965462	2	37	theme	crystallinity	392:404	arg1	index					406:410	crystallinity index	392:410	crystallinity index (CI)	392:415	The CNF was in rod shape with the diameter of 23-38 nm and the length of 125-217 nm and crystallinity index (CI) was 0.89.
25965462	3	38	theme	nanocomposites	612:625	arg1	optical					501:507	optical	501:507	optical	501:507	The effect of CNF concentration (1, 3, 5, and 10 wt% based on CMC) on the optical, morphological, mechanical, water vapor barrier, surface hydrophobicity, and thermal properties of the nanocomposites were studied.
25965462	4	39	with	compatible	757:766	arg1	CMC					777:779	the CMC	773:779	the CMC	773:779	The CNF was evenly distributed in the polymer matrix to form smooth and flexible films indicating the CNF is highly compatible with the CMC.
25965462	2	40	theme	nm	385:386	arg1	length					367:372	the length	363:372	the length of 125-217 nm and crystallinity index (CI)	363:415	The CNF was in rod shape with the diameter of 23-38 nm and the length of 125-217 nm and crystallinity index (CI) was 0.89.
25965462	2	40	theme	nm	385:386	arg1	0.89					421:424	0.89	421:424	0.89	421:424	The CNF was in rod shape with the diameter of 23-38 nm and the length of 125-217 nm and crystallinity index (CI) was 0.89.
25965462	0	41	theme	carboxymethyl	43:55	arg1	cellulose/cotton					57:72	sodium carboxymethyl cellulose/cotton	36:72	sodium carboxymethyl cellulose/cotton	36:72	Preparation and characterization of sodium carboxymethyl cellulose/cotton linter cellulose nanofibril composite films.
25965462	1	42	used	used	231:234	arg2	nanofibril					141:150	Crystalline cellulose nanofibril	119:150	Crystalline cellulose nanofibril (CNF)	119:156	Crystalline cellulose nanofibril (CNF) was isolated from cotton linter pulp using an acid hydrolysis method and used as a filler to reinforce sodium carboxymethyl cellulose (CMC) film.
25965462	1	42	used	used	231:234	arg2	CNF					153:155	CNF	153:155	CNF	153:155	Crystalline cellulose nanofibril (CNF) was isolated from cotton linter pulp using an acid hydrolysis method and used as a filler to reinforce sodium carboxymethyl cellulose (CMC) film.
25965462	1	42	used	used	231:234	arg2	filler					241:246	a filler	239:246	a filler	239:246	Crystalline cellulose nanofibril (CNF) was isolated from cotton linter pulp using an acid hydrolysis method and used as a filler to reinforce sodium carboxymethyl cellulose (CMC) film.
25965462	2	43	theme	nm	356:357	arg1	diameter					338:345	the diameter	334:345	the diameter of 23-38 nm	334:357	The CNF was in rod shape with the diameter of 23-38 nm and the length of 125-217 nm and crystallinity index (CI) was 0.89.
25965462	0	44	theme	sodium	36:41	arg1	cellulose/cotton					57:72	sodium carboxymethyl cellulose/cotton	36:72	sodium carboxymethyl cellulose/cotton	36:72	Preparation and characterization of sodium carboxymethyl cellulose/cotton linter cellulose nanofibril composite films.
25965462	6	45	theme	CMC	1105:1107	arg1	film					1109:1112	the control CMC film	1093:1112	the control CMC film	1093:1112	The WVP of CMC film decreased at low content of CNF, and increased with increase in CNF content, then decreased but to the same level of the control CMC film with the inclusion of 10 wt% of CNF.
25965462	7	46	theme	CNF	1229:1231	arg1	CNF					1229:1231	CNF	1229:1231	CNF	1229:1231	Transparency of CMC film decreased slightly from 87.7% to 86.2% with 5 wt% of CNF.
25965462	7	46	theme	CNF	1229:1231	arg1	%					1224:1224	5 wt%	1220:1224	5 wt% of CNF	1220:1231	Transparency of CMC film decreased slightly from 87.7% to 86.2% with 5 wt% of CNF.
25965462	6	47	theme	wt	1139:1140	arg1	CNF					1146:1148	CNF	1146:1148	CNF	1146:1148	The WVP of CMC film decreased at low content of CNF, and increased with increase in CNF content, then decreased but to the same level of the control CMC film with the inclusion of 10 wt% of CNF.
25965462	6	47	theme	wt	1139:1140	arg1	%					1141:1141	10 wt%	1136:1141	10 wt% of CNF	1136:1148	The WVP of CMC film decreased at low content of CNF, and increased with increase in CNF content, then decreased but to the same level of the control CMC film with the inclusion of 10 wt% of CNF.
25965462	8	48	theme	processed	1390:1398	arg1	fruits					1400:1405	fresh and minimally processed fruits	1370:1405	fresh and minimally processed fruits	1370:1405	The CMC/CNF composite films have a high potential to be used as an edible coating or packaging films for the extension of shelf life of fresh and minimally processed fruits and vegetables.
25965462	8	49	theme	fresh	1370:1374	arg1	fruits					1400:1405	fresh and minimally processed fruits	1370:1405	fresh and minimally processed fruits	1370:1405	The CMC/CNF composite films have a high potential to be used as an edible coating or packaging films for the extension of shelf life of fresh and minimally processed fruits and vegetables.
25965462	2	50	with	shape	323:327	arg1	diameter					338:345	the diameter	334:345	the diameter of 23-38 nm	334:357	The CNF was in rod shape with the diameter of 23-38 nm and the length of 125-217 nm and crystallinity index (CI) was 0.89.
25965462	4	51	theme	smooth	702:707	arg1	films					722:726	smooth and flexible films	702:726	smooth and flexible films	702:726	The CNF was evenly distributed in the polymer matrix to form smooth and flexible films indicating the CNF is highly compatible with the CMC.
25965462	7	52	theme	wt	1222:1223	arg1	CNF					1229:1231	CNF	1229:1231	CNF	1229:1231	Transparency of CMC film decreased slightly from 87.7% to 86.2% with 5 wt% of CNF.
25965462	7	52	theme	wt	1222:1223	arg1	%					1224:1224	5 wt%	1220:1224	5 wt% of CNF	1220:1231	Transparency of CMC film decreased slightly from 87.7% to 86.2% with 5 wt% of CNF.
25965462	0	53	theme	cellulose/cotton	57:72	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of sodium carboxymethyl cellulose/cotton linter cellulose nanofibril composite films.
25965462	0	53	theme	cellulose/cotton	57:72	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of sodium carboxymethyl cellulose/cotton linter cellulose nanofibril composite films.
25965462	5	54	theme	wt	934:935	arg1	%					936:936	5 wt%	932:936	5 wt% of CNF inclusion	932:953	The tensile strength (TS) and elastic modulus (EM) of CMC film increased by 23% and 27%, respectively, while the elongation (E) decreased by 28% with 5 wt% of CNF inclusion.
25965462	5	54	theme	wt	934:935	arg1	inclusion					945:953	CNF inclusion	941:953	CNF inclusion	941:953	The tensile strength (TS) and elastic modulus (EM) of CMC film increased by 23% and 27%, respectively, while the elongation (E) decreased by 28% with 5 wt% of CNF inclusion.
25965462	7	55	theme	%	1204:1204	arg1	%					1213:1213	87.7% to 86.2%	1200:1213	87.7% to 86.2%	1200:1213	Transparency of CMC film decreased slightly from 87.7% to 86.2% with 5 wt% of CNF.
25965462	6	56	theme	film	971:974	arg1	WVP					960:962	The WVP	956:962	The WVP of CMC film	956:974	The WVP of CMC film decreased at low content of CNF, and increased with increase in CNF content, then decreased but to the same level of the control CMC film with the inclusion of 10 wt% of CNF.
25965462	3	57	theme	CNF	441:443	arg1	concentration					445:457	CNF concentration	441:457	CNF concentration (1, 3, 5, and 10 wt% based on CMC)	441:492	The effect of CNF concentration (1, 3, 5, and 10 wt% based on CMC) on the optical, morphological, mechanical, water vapor barrier, surface hydrophobicity, and thermal properties of the nanocomposites were studied.
25965462	8	58	theme	CMC/CNF	1238:1244	arg1	films					1256:1260	The CMC/CNF composite films	1234:1260	The CMC/CNF composite films	1234:1260	The CMC/CNF composite films have a high potential to be used as an edible coating or packaging films for the extension of shelf life of fresh and minimally processed fruits and vegetables.
25965462	6	59	theme	CNF	1040:1042	arg1	content					1044:1050	CNF content	1040:1050	CNF content	1040:1050	The WVP of CMC film decreased at low content of CNF, and increased with increase in CNF content, then decreased but to the same level of the control CMC film with the inclusion of 10 wt% of CNF.
25965462	6	60	theme	CMC	967:969	arg1	film					971:974	CMC film	967:974	CMC film	967:974	The WVP of CMC film decreased at low content of CNF, and increased with increase in CNF content, then decreased but to the same level of the control CMC film with the inclusion of 10 wt% of CNF.
25965462	8	61	theme	fruits	1400:1405	arg1	extension					1343:1351	the extension	1339:1351	the extension of shelf life of fresh and minimally processed fruits and vegetables	1339:1420	The CMC/CNF composite films have a high potential to be used as an edible coating or packaging films for the extension of shelf life of fresh and minimally processed fruits and vegetables.
25965462	2	62	theme	rod	319:321	arg1	shape					323:327	rod shape	319:327	rod shape	319:327	The CNF was in rod shape with the diameter of 23-38 nm and the length of 125-217 nm and crystallinity index (CI) was 0.89.
25965462	3	63	theme	water	537:541	arg1	barrier					549:555	water vapor barrier	537:555	water vapor barrier	537:555	The effect of CNF concentration (1, 3, 5, and 10 wt% based on CMC) on the optical, morphological, mechanical, water vapor barrier, surface hydrophobicity, and thermal properties of the nanocomposites were studied.
25965462	6	64	theme	film	1109:1112	arg1	level					1084:1088	the same level	1075:1088	the same level of the control CMC film	1075:1112	The WVP of CMC film decreased at low content of CNF, and increased with increase in CNF content, then decreased but to the same level of the control CMC film with the inclusion of 10 wt% of CNF.
25965462	8	65	theme	life	1362:1365	arg1	extension					1343:1351	the extension	1339:1351	the extension of shelf life of fresh and minimally processed fruits and vegetables	1339:1420	The CMC/CNF composite films have a high potential to be used as an edible coating or packaging films for the extension of shelf life of fresh and minimally processed fruits and vegetables.
25965462	5	66	theme	tensile	786:792	arg1	TS					804:805	TS	804:805	TS	804:805	The tensile strength (TS) and elastic modulus (EM) of CMC film increased by 23% and 27%, respectively, while the elongation (E) decreased by 28% with 5 wt% of CNF inclusion.
25965462	5	66	theme	tensile	786:792	arg1	strength					794:801	The tensile strength	782:801	The tensile strength (TS)	782:806	The tensile strength (TS) and elastic modulus (EM) of CMC film increased by 23% and 27%, respectively, while the elongation (E) decreased by 28% with 5 wt% of CNF inclusion.
25965462	0	67	theme	nanofibril	91:100	arg1	films					112:116	cellulose nanofibril composite films	81:116	cellulose nanofibril composite films	81:116	Preparation and characterization of sodium carboxymethyl cellulose/cotton linter cellulose nanofibril composite films.
25965462	3	68	theme	vapor	543:547	arg1	barrier					549:555	water vapor barrier	537:555	water vapor barrier	537:555	The effect of CNF concentration (1, 3, 5, and 10 wt% based on CMC) on the optical, morphological, mechanical, water vapor barrier, surface hydrophobicity, and thermal properties of the nanocomposites were studied.
25965462	4	69	theme	polymer	679:685	arg1	matrix					687:692	the polymer matrix	675:692	the polymer matrix	675:692	The CNF was evenly distributed in the polymer matrix to form smooth and flexible films indicating the CNF is highly compatible with the CMC.
25965462	8	70	theme	vegetables	1411:1420	arg1	extension					1343:1351	the extension	1339:1351	the extension of shelf life of fresh and minimally processed fruits and vegetables	1339:1420	The CMC/CNF composite films have a high potential to be used as an edible coating or packaging films for the extension of shelf life of fresh and minimally processed fruits and vegetables.
25965462	8	71	theme	edible	1301:1306	arg1	coating					1308:1314	an edible coating	1298:1314	an edible coating	1298:1314	The CMC/CNF composite films have a high potential to be used as an edible coating or packaging films for the extension of shelf life of fresh and minimally processed fruits and vegetables.
25965462	6	72	from	increase	1028:1035	arg1	content					1044:1050	CNF content	1040:1050	CNF content	1040:1050	The WVP of CMC film decreased at low content of CNF, and increased with increase in CNF content, then decreased but to the same level of the control CMC film with the inclusion of 10 wt% of CNF.
25965462	0	73	theme	cellulose	81:89	arg1	films					112:116	cellulose nanofibril composite films	81:116	cellulose nanofibril composite films	81:116	Preparation and characterization of sodium carboxymethyl cellulose/cotton linter cellulose nanofibril composite films.
25965462	5	74	theme	film	840:843	arg1	modulus					820:826	elastic modulus	812:826	elastic modulus (EM) of CMC film	812:843	The tensile strength (TS) and elastic modulus (EM) of CMC film increased by 23% and 27%, respectively, while the elongation (E) decreased by 28% with 5 wt% of CNF inclusion.
25965462	5	74	theme	film	840:843	arg1	EM					829:830	EM	829:830	EM	829:830	The tensile strength (TS) and elastic modulus (EM) of CMC film increased by 23% and 27%, respectively, while the elongation (E) decreased by 28% with 5 wt% of CNF inclusion.
25965462	5	74	theme	film	840:843	arg1	TS					804:805	TS	804:805	TS	804:805	The tensile strength (TS) and elastic modulus (EM) of CMC film increased by 23% and 27%, respectively, while the elongation (E) decreased by 28% with 5 wt% of CNF inclusion.
25965462	5	74	theme	film	840:843	arg1	strength					794:801	The tensile strength	782:801	The tensile strength (TS)	782:806	The tensile strength (TS) and elastic modulus (EM) of CMC film increased by 23% and 27%, respectively, while the elongation (E) decreased by 28% with 5 wt% of CNF inclusion.
25965462	8	75	theme	packaging	1319:1327	arg1	films					1329:1333	packaging films	1319:1333	packaging films	1319:1333	The CMC/CNF composite films have a high potential to be used as an edible coating or packaging films for the extension of shelf life of fresh and minimally processed fruits and vegetables.
26971618	10	0	theme	genotoxic	1775:1783	arg1	effects					1785:1791	hematotoxic and genotoxic effects	1759:1791	hematotoxic and genotoxic effects induced by PP	1759:1805	DISCUSSION AND CONCLUSION A decrease in hematotoxic and genotoxic effects induced by PP is due to its powerful antioxidant capacity.
26971618	6	1	theme	hemoglobin	1088:1097	arg1	%					1109:1109	8%	1108:1109	8%	1108:1109	In vivo study showed a decrease in red blood cells (70%) and platelet counts (46%), hemoglobin content (8%), hematocrit percent (7%), and an 80% increase of white blood cells in Ba-treated rats.
26971618	6	1	theme	hemoglobin	1088:1097	arg1	content					1099:1105	hemoglobin content	1088:1105	hemoglobin content (8%)	1088:1110	In vivo study showed a decrease in red blood cells (70%) and platelet counts (46%), hemoglobin content (8%), hematocrit percent (7%), and an 80% increase of white blood cells in Ba-treated rats.
26971618	2	2	theme	PP	372:373	arg1	effects					361:367	protective effects	350:367	protective effects	350:367	OBJECTIVE This study investigated the biochemical composition and protective effects of PP against hematotoxicity and genotoxicity induced by barium chloride (BaCl2) in adult rats.
26971618	2	2	theme	PP	372:373	arg1	composition					334:344	biochemical composition	322:344	biochemical composition	322:344	OBJECTIVE This study investigated the biochemical composition and protective effects of PP against hematotoxicity and genotoxicity induced by barium chloride (BaCl2) in adult rats.
26971618	6	3	theme	red	1039:1041	arg1	cells					1049:1053	red blood cells	1039:1053	red blood cells (70%)	1039:1059	In vivo study showed a decrease in red blood cells (70%) and platelet counts (46%), hemoglobin content (8%), hematocrit percent (7%), and an 80% increase of white blood cells in Ba-treated rats.
26971618	6	3	theme	red	1039:1041	arg1	%					1058:1058	70%	1056:1058	70%	1056:1058	In vivo study showed a decrease in red blood cells (70%) and platelet counts (46%), hemoglobin content (8%), hematocrit percent (7%), and an 80% increase of white blood cells in Ba-treated rats.
26971618	10	4	theme	hematotoxic	1759:1769	arg1	effects					1785:1791	hematotoxic and genotoxic effects	1759:1791	hematotoxic and genotoxic effects induced by PP	1759:1805	DISCUSSION AND CONCLUSION A decrease in hematotoxic and genotoxic effects induced by PP is due to its powerful antioxidant capacity.
26971618	8	5	theme	aberrations	1531:1541	arg1	frequencies					1498:1508	the frequencies	1494:1508	the frequencies of total chromosomal aberrations with abnormal metaphases	1494:1566	BaCl2-treatment showed a significant increase in the frequencies of total chromosomal aberrations with abnormal metaphases and micronucleus in bone-marrow cells.
26971618	8	6	theme	abnormal	1548:1555	arg1	metaphases					1557:1566	abnormal metaphases	1548:1566	abnormal metaphases	1548:1566	BaCl2-treatment showed a significant increase in the frequencies of total chromosomal aberrations with abnormal metaphases and micronucleus in bone-marrow cells.
26971618	0	7	theme	Protective	0:9	arg1	effects					11:17	Protective effects	0:17	Protective effects of pomegranate	0:32	Protective effects of pomegranate peel against hematotoxicity, chromosomal aberrations, and genotoxicity induced by barium chloride in adult rats.
26971618	2	8	theme	biochemical	322:332	arg1	composition					334:344	biochemical composition	322:344	biochemical composition	322:344	OBJECTIVE This study investigated the biochemical composition and protective effects of PP against hematotoxicity and genotoxicity induced by barium chloride (BaCl2) in adult rats.
26971618	8	9	theme	total	1513:1517	arg1	aberrations					1531:1541	total chromosomal aberrations	1513:1541	total chromosomal aberrations with abnormal metaphases	1513:1566	BaCl2-treatment showed a significant increase in the frequencies of total chromosomal aberrations with abnormal metaphases and micronucleus in bone-marrow cells.
26971618	7	10	from	reduction	1201:1209	arg1	glutathione					1279:1289	glutathione	1279:1289	glutathione	1279:1289	A reduction in antioxidant status: catalase, glutathione peroxidase activities, glutathione, and vitamin C levels by 31, 21, 28, and 29%, respectively, and an increase in MDA (46%) and AOPP levels (72%) were also observed compared with controls.
26971618	7	10	from	reduction	1201:1209	arg1	%					1399:1399	72%	1397:1399	72%	1397:1399	A reduction in antioxidant status: catalase, glutathione peroxidase activities, glutathione, and vitamin C levels by 31, 21, 28, and 29%, respectively, and an increase in MDA (46%) and AOPP levels (72%) were also observed compared with controls.
26971618	7	10	from	reduction	1201:1209	arg1	activities					1267:1276	glutathione peroxidase activities	1244:1276	glutathione peroxidase activities	1244:1276	A reduction in antioxidant status: catalase, glutathione peroxidase activities, glutathione, and vitamin C levels by 31, 21, 28, and 29%, respectively, and an increase in MDA (46%) and AOPP levels (72%) were also observed compared with controls.
26971618	7	10	from	reduction	1201:1209	arg1	MDA					1370:1372	MDA	1370:1372	MDA (46%)	1370:1378	A reduction in antioxidant status: catalase, glutathione peroxidase activities, glutathione, and vitamin C levels by 31, 21, 28, and 29%, respectively, and an increase in MDA (46%) and AOPP levels (72%) were also observed compared with controls.
26971618	7	10	from	reduction	1201:1209	arg1	levels					1306:1311	vitamin C levels	1296:1311	vitamin C levels	1296:1311	A reduction in antioxidant status: catalase, glutathione peroxidase activities, glutathione, and vitamin C levels by 31, 21, 28, and 29%, respectively, and an increase in MDA (46%) and AOPP levels (72%) were also observed compared with controls.
26971618	7	10	from	reduction	1201:1209	arg1	status					1226:1231	antioxidant status	1214:1231	antioxidant status	1214:1231	A reduction in antioxidant status: catalase, glutathione peroxidase activities, glutathione, and vitamin C levels by 31, 21, 28, and 29%, respectively, and an increase in MDA (46%) and AOPP levels (72%) were also observed compared with controls.
26971618	7	10	from	reduction	1201:1209	arg1	%					1377:1377	46%	1375:1377	46%	1375:1377	A reduction in antioxidant status: catalase, glutathione peroxidase activities, glutathione, and vitamin C levels by 31, 21, 28, and 29%, respectively, and an increase in MDA (46%) and AOPP levels (72%) were also observed compared with controls.
26971618	7	10	from	reduction	1201:1209	arg1	levels					1389:1394	AOPP levels	1384:1394	AOPP levels (72%)	1384:1400	A reduction in antioxidant status: catalase, glutathione peroxidase activities, glutathione, and vitamin C levels by 31, 21, 28, and 29%, respectively, and an increase in MDA (46%) and AOPP levels (72%) were also observed compared with controls.
26971618	7	10	from	reduction	1201:1209	arg1	catalase					1234:1241	catalase	1234:1241	catalase	1234:1241	A reduction in antioxidant status: catalase, glutathione peroxidase activities, glutathione, and vitamin C levels by 31, 21, 28, and 29%, respectively, and an increase in MDA (46%) and AOPP levels (72%) were also observed compared with controls.
26971618	3	11	dep	PP	593:594	arg1	%					598:598	5%	597:598	5% via diet	597:607	MATERIALS AND METHODS Adult Wistar rats were divided into four groups of six each: control, barium (67 ppm via drinking water), PP (5% via diet), and their combination during 21 d. Oxidative stress was determined by MDA, AOPP, and antioxidant status: CAT, GPx, GSH, Vit C. Osmotic fragility (OF), chromosomal aberrations (CAs), and micronucleus (MN) assays were also studied.
26971618	6	12	theme	platelet	1065:1072	arg1	counts					1074:1079	platelet counts	1065:1079	platelet counts (46%)	1065:1085	In vivo study showed a decrease in red blood cells (70%) and platelet counts (46%), hemoglobin content (8%), hematocrit percent (7%), and an 80% increase of white blood cells in Ba-treated rats.
26971618	6	12	theme	platelet	1065:1072	arg1	%					1084:1084	46%	1082:1084	46%	1082:1084	In vivo study showed a decrease in red blood cells (70%) and platelet counts (46%), hemoglobin content (8%), hematocrit percent (7%), and an 80% increase of white blood cells in Ba-treated rats.
26971618	0	13	theme	adult	135:139	arg1	rats					141:144	adult rats	135:144	adult rats	135:144	Protective effects of pomegranate peel against hematotoxicity, chromosomal aberrations, and genotoxicity induced by barium chloride in adult rats.
26971618	1	14	theme	antibacterial	207:219	arg1	properties					272:281	antibacterial, antioxidant, anti-inflammatory, and antimutagenic properties	207:281	antibacterial, antioxidant, anti-inflammatory, and antimutagenic properties	207:281	CONTEXT Pomegranate peel (PP) has health benefits including antibacterial, antioxidant, anti-inflammatory, and antimutagenic properties.
26971618	10	15	dep	DISCUSSION	1719:1728	arg1	decrease					1747:1754	A decrease	1745:1754	DISCUSSION AND CONCLUSION A decrease in hematotoxic and genotoxic effects induced by PP	1719:1805	DISCUSSION AND CONCLUSION A decrease in hematotoxic and genotoxic effects induced by PP is due to its powerful antioxidant capacity.
26971618	7	16	from	increase	1358:1365	arg1	glutathione					1279:1289	glutathione	1279:1289	glutathione	1279:1289	A reduction in antioxidant status: catalase, glutathione peroxidase activities, glutathione, and vitamin C levels by 31, 21, 28, and 29%, respectively, and an increase in MDA (46%) and AOPP levels (72%) were also observed compared with controls.
26971618	7	16	from	increase	1358:1365	arg1	%					1399:1399	72%	1397:1399	72%	1397:1399	A reduction in antioxidant status: catalase, glutathione peroxidase activities, glutathione, and vitamin C levels by 31, 21, 28, and 29%, respectively, and an increase in MDA (46%) and AOPP levels (72%) were also observed compared with controls.
26971618	7	16	from	increase	1358:1365	arg1	activities					1267:1276	glutathione peroxidase activities	1244:1276	glutathione peroxidase activities	1244:1276	A reduction in antioxidant status: catalase, glutathione peroxidase activities, glutathione, and vitamin C levels by 31, 21, 28, and 29%, respectively, and an increase in MDA (46%) and AOPP levels (72%) were also observed compared with controls.
26971618	7	16	from	increase	1358:1365	arg1	levels					1389:1394	AOPP levels	1384:1394	AOPP levels (72%)	1384:1400	A reduction in antioxidant status: catalase, glutathione peroxidase activities, glutathione, and vitamin C levels by 31, 21, 28, and 29%, respectively, and an increase in MDA (46%) and AOPP levels (72%) were also observed compared with controls.
26971618	7	16	from	increase	1358:1365	arg1	MDA					1370:1372	MDA	1370:1372	MDA (46%)	1370:1378	A reduction in antioxidant status: catalase, glutathione peroxidase activities, glutathione, and vitamin C levels by 31, 21, 28, and 29%, respectively, and an increase in MDA (46%) and AOPP levels (72%) were also observed compared with controls.
26971618	7	16	from	increase	1358:1365	arg1	levels					1306:1311	vitamin C levels	1296:1311	vitamin C levels	1296:1311	A reduction in antioxidant status: catalase, glutathione peroxidase activities, glutathione, and vitamin C levels by 31, 21, 28, and 29%, respectively, and an increase in MDA (46%) and AOPP levels (72%) were also observed compared with controls.
26971618	7	16	from	increase	1358:1365	arg1	status					1226:1231	antioxidant status	1214:1231	antioxidant status	1214:1231	A reduction in antioxidant status: catalase, glutathione peroxidase activities, glutathione, and vitamin C levels by 31, 21, 28, and 29%, respectively, and an increase in MDA (46%) and AOPP levels (72%) were also observed compared with controls.
26971618	7	16	from	increase	1358:1365	arg1	%					1377:1377	46%	1375:1377	46%	1375:1377	A reduction in antioxidant status: catalase, glutathione peroxidase activities, glutathione, and vitamin C levels by 31, 21, 28, and 29%, respectively, and an increase in MDA (46%) and AOPP levels (72%) were also observed compared with controls.
26971618	7	16	from	increase	1358:1365	arg1	catalase					1234:1241	catalase	1234:1241	catalase	1234:1241	A reduction in antioxidant status: catalase, glutathione peroxidase activities, glutathione, and vitamin C levels by 31, 21, 28, and 29%, respectively, and an increase in MDA (46%) and AOPP levels (72%) were also observed compared with controls.
26971618	8	17	from	increase	1482:1489	arg1	cells					1600:1604	bone-marrow cells	1588:1604	bone-marrow cells	1588:1604	BaCl2-treatment showed a significant increase in the frequencies of total chromosomal aberrations with abnormal metaphases and micronucleus in bone-marrow cells.
26971618	8	17	from	increase	1482:1489	arg1	frequencies					1498:1508	the frequencies	1494:1508	the frequencies of total chromosomal aberrations with abnormal metaphases	1494:1566	BaCl2-treatment showed a significant increase in the frequencies of total chromosomal aberrations with abnormal metaphases and micronucleus in bone-marrow cells.
26971618	4	18	dep	RESULTS	841:847	arg1	PP					849:850	PP	849:850	RESULTS PP	841:850	RESULTS PP showed a rich composition of antioxidant compounds.
26971618	5	19	theme	DPPH	904:907	arg1	test					909:912	DPPH test	904:912	DPPH test	904:912	DPPH test found IC50 value= 5.3 μg/mL and a high polysaccharides content (315 ± 5 mg/g of extract).
26971618	3	20	theme	drinking	576:583	arg1	water					585:589	drinking water	576:589	drinking water	576:589	MATERIALS AND METHODS Adult Wistar rats were divided into four groups of six each: control, barium (67 ppm via drinking water), PP (5% via diet), and their combination during 21 d. Oxidative stress was determined by MDA, AOPP, and antioxidant status: CAT, GPx, GSH, Vit C. Osmotic fragility (OF), chromosomal aberrations (CAs), and micronucleus (MN) assays were also studied.
26971618	10	21	theme	antioxidant	1830:1840	arg1	capacity					1842:1849	its powerful antioxidant capacity	1817:1849	its powerful antioxidant capacity	1817:1849	DISCUSSION AND CONCLUSION A decrease in hematotoxic and genotoxic effects induced by PP is due to its powerful antioxidant capacity.
26971618	2	22	dep	composition	334:344	arg1	the					318:320	the	318:320	the	318:320	OBJECTIVE This study investigated the biochemical composition and protective effects of PP against hematotoxicity and genotoxicity induced by barium chloride (BaCl2) in adult rats.
26971618	3	23	theme	GPx	721:723	arg1	assays					815:820	CAT, GPx, GSH, Vit C. Osmotic fragility (OF), chromosomal aberrations (CAs), and micronucleus (MN) assays	716:820	CAT, GPx, GSH, Vit C. Osmotic fragility (OF), chromosomal aberrations (CAs), and micronucleus (MN) assays	716:820	MATERIALS AND METHODS Adult Wistar rats were divided into four groups of six each: control, barium (67 ppm via drinking water), PP (5% via diet), and their combination during 21 d. Oxidative stress was determined by MDA, AOPP, and antioxidant status: CAT, GPx, GSH, Vit C. Osmotic fragility (OF), chromosomal aberrations (CAs), and micronucleus (MN) assays were also studied.
26971618	3	24	theme	Osmotic	738:744	arg1	fragility					746:754	Vit C. Osmotic fragility	731:754	Vit C. Osmotic fragility (OF)	731:759	MATERIALS AND METHODS Adult Wistar rats were divided into four groups of six each: control, barium (67 ppm via drinking water), PP (5% via diet), and their combination during 21 d. Oxidative stress was determined by MDA, AOPP, and antioxidant status: CAT, GPx, GSH, Vit C. Osmotic fragility (OF), chromosomal aberrations (CAs), and micronucleus (MN) assays were also studied.
26971618	2	25	dep	OBJECTIVE	284:292	arg1	investigated					305:316	investigated	305:316	investigated the biochemical composition and protective effects of PP against hematotoxicity and genotoxicity induced by barium chloride (BaCl2) in adult rats	305:462	OBJECTIVE This study investigated the biochemical composition and protective effects of PP against hematotoxicity and genotoxicity induced by barium chloride (BaCl2) in adult rats.
26971618	3	26	theme	CAT	716:718	arg1	assays					815:820	CAT, GPx, GSH, Vit C. Osmotic fragility (OF), chromosomal aberrations (CAs), and micronucleus (MN) assays	716:820	CAT, GPx, GSH, Vit C. Osmotic fragility (OF), chromosomal aberrations (CAs), and micronucleus (MN) assays	716:820	MATERIALS AND METHODS Adult Wistar rats were divided into four groups of six each: control, barium (67 ppm via drinking water), PP (5% via diet), and their combination during 21 d. Oxidative stress was determined by MDA, AOPP, and antioxidant status: CAT, GPx, GSH, Vit C. Osmotic fragility (OF), chromosomal aberrations (CAs), and micronucleus (MN) assays were also studied.
26971618	6	27	theme	80	1145:1146	arg1	%					1147:1147	%	1147:1147	%	1147:1147	In vivo study showed a decrease in red blood cells (70%) and platelet counts (46%), hemoglobin content (8%), hematocrit percent (7%), and an 80% increase of white blood cells in Ba-treated rats.
26971618	3	28	dep	barium	557:562	arg1	67 ppm					565:570	67 ppm	565:570	67 ppm via drinking water	565:589	MATERIALS AND METHODS Adult Wistar rats were divided into four groups of six each: control, barium (67 ppm via drinking water), PP (5% via diet), and their combination during 21 d. Oxidative stress was determined by MDA, AOPP, and antioxidant status: CAT, GPx, GSH, Vit C. Osmotic fragility (OF), chromosomal aberrations (CAs), and micronucleus (MN) assays were also studied.
26971618	7	29	theme	AOPP	1384:1387	arg1	levels					1389:1394	AOPP levels	1384:1394	AOPP levels (72%)	1384:1400	A reduction in antioxidant status: catalase, glutathione peroxidase activities, glutathione, and vitamin C levels by 31, 21, 28, and 29%, respectively, and an increase in MDA (46%) and AOPP levels (72%) were also observed compared with controls.
26971618	7	29	theme	AOPP	1384:1387	arg1	%					1399:1399	72%	1397:1399	72%	1397:1399	A reduction in antioxidant status: catalase, glutathione peroxidase activities, glutathione, and vitamin C levels by 31, 21, 28, and 29%, respectively, and an increase in MDA (46%) and AOPP levels (72%) were also observed compared with controls.
26971618	9	30	theme	chromosomal	1670:1680	arg1	abnormalities					1682:1694	chromosomal abnormalities	1670:1694	chromosomal abnormalities leading to DNA damage	1670:1716	Oxidative stress induced by BaCl2 might be the major cause for chromosomal abnormalities leading to DNA damage.
26971618	3	31	theme	Vit	731:733	arg1	fragility					746:754	Vit C. Osmotic fragility	731:754	Vit C. Osmotic fragility (OF)	731:759	MATERIALS AND METHODS Adult Wistar rats were divided into four groups of six each: control, barium (67 ppm via drinking water), PP (5% via diet), and their combination during 21 d. Oxidative stress was determined by MDA, AOPP, and antioxidant status: CAT, GPx, GSH, Vit C. Osmotic fragility (OF), chromosomal aberrations (CAs), and micronucleus (MN) assays were also studied.
26971618	3	32	theme	GSH	726:728	arg1	assays					815:820	CAT, GPx, GSH, Vit C. Osmotic fragility (OF), chromosomal aberrations (CAs), and micronucleus (MN) assays	716:820	CAT, GPx, GSH, Vit C. Osmotic fragility (OF), chromosomal aberrations (CAs), and micronucleus (MN) assays	716:820	MATERIALS AND METHODS Adult Wistar rats were divided into four groups of six each: control, barium (67 ppm via drinking water), PP (5% via diet), and their combination during 21 d. Oxidative stress was determined by MDA, AOPP, and antioxidant status: CAT, GPx, GSH, Vit C. Osmotic fragility (OF), chromosomal aberrations (CAs), and micronucleus (MN) assays were also studied.
26971618	5	33	theme	polysaccharides	953:967	arg1	content					969:975	a high polysaccharides content	946:975	a high polysaccharides content (315 ± 5 mg/g of extract)	946:1001	DPPH test found IC50 value= 5.3 μg/mL and a high polysaccharides content (315 ± 5 mg/g of extract).
26971618	7	34	theme	C	1304:1304	arg1	levels					1306:1311	vitamin C levels	1296:1311	vitamin C levels	1296:1311	A reduction in antioxidant status: catalase, glutathione peroxidase activities, glutathione, and vitamin C levels by 31, 21, 28, and 29%, respectively, and an increase in MDA (46%) and AOPP levels (72%) were also observed compared with controls.
26971618	9	35	theme	DNA	1707:1709	arg1	damage					1711:1716	DNA damage	1707:1716	DNA damage	1707:1716	Oxidative stress induced by BaCl2 might be the major cause for chromosomal abnormalities leading to DNA damage.
26971618	8	36	with	aberrations	1531:1541	arg1	metaphases					1557:1566	abnormal metaphases	1548:1566	abnormal metaphases	1548:1566	BaCl2-treatment showed a significant increase in the frequencies of total chromosomal aberrations with abnormal metaphases and micronucleus in bone-marrow cells.
26971618	2	37	theme	barium	426:431	arg1	BaCl2					443:447	BaCl2	443:447	BaCl2	443:447	OBJECTIVE This study investigated the biochemical composition and protective effects of PP against hematotoxicity and genotoxicity induced by barium chloride (BaCl2) in adult rats.
26971618	2	37	theme	barium	426:431	arg1	chloride					433:440	barium chloride	426:440	barium chloride (BaCl2)	426:448	OBJECTIVE This study investigated the biochemical composition and protective effects of PP against hematotoxicity and genotoxicity induced by barium chloride (BaCl2) in adult rats.
26971618	4	38	theme	antioxidant	881:891	arg1	compounds					893:901	antioxidant compounds	881:901	antioxidant compounds	881:901	RESULTS PP showed a rich composition of antioxidant compounds.
26971618	6	39	theme	Ba-treated	1182:1191	arg1	rats					1193:1196	Ba-treated rats	1182:1196	Ba-treated rats	1182:1196	In vivo study showed a decrease in red blood cells (70%) and platelet counts (46%), hemoglobin content (8%), hematocrit percent (7%), and an 80% increase of white blood cells in Ba-treated rats.
26971618	2	40	theme	adult	453:457	arg1	rats					459:462	adult rats	453:462	adult rats	453:462	OBJECTIVE This study investigated the biochemical composition and protective effects of PP against hematotoxicity and genotoxicity induced by barium chloride (BaCl2) in adult rats.
26971618	6	41	theme	cells	1173:1177	arg1	percent					1124:1130	hematocrit percent	1113:1130	hematocrit percent (7%)	1113:1135	In vivo study showed a decrease in red blood cells (70%) and platelet counts (46%), hemoglobin content (8%), hematocrit percent (7%), and an 80% increase of white blood cells in Ba-treated rats.
26971618	6	41	theme	cells	1173:1177	arg1	decrease					1027:1034	a decrease	1025:1034	a decrease in red blood cells (70%) and platelet counts (46%)	1025:1085	In vivo study showed a decrease in red blood cells (70%) and platelet counts (46%), hemoglobin content (8%), hematocrit percent (7%), and an 80% increase of white blood cells in Ba-treated rats.
26971618	6	41	theme	cells	1173:1177	arg1	%					1109:1109	8%	1108:1109	8%	1108:1109	In vivo study showed a decrease in red blood cells (70%) and platelet counts (46%), hemoglobin content (8%), hematocrit percent (7%), and an 80% increase of white blood cells in Ba-treated rats.
26971618	6	41	theme	cells	1173:1177	arg1	%					1134:1134	7%	1133:1134	7%	1133:1134	In vivo study showed a decrease in red blood cells (70%) and platelet counts (46%), hemoglobin content (8%), hematocrit percent (7%), and an 80% increase of white blood cells in Ba-treated rats.
26971618	6	41	theme	cells	1173:1177	arg1	increase					1149:1156	an 80% increase	1142:1156	an 80% increase of white blood cells in Ba-treated rats	1142:1196	In vivo study showed a decrease in red blood cells (70%) and platelet counts (46%), hemoglobin content (8%), hematocrit percent (7%), and an 80% increase of white blood cells in Ba-treated rats.
26971618	6	41	theme	cells	1173:1177	arg1	content					1099:1105	hemoglobin content	1088:1105	hemoglobin content (8%)	1088:1110	In vivo study showed a decrease in red blood cells (70%) and platelet counts (46%), hemoglobin content (8%), hematocrit percent (7%), and an 80% increase of white blood cells in Ba-treated rats.
26971618	6	42	theme	white	1161:1165	arg1	cells					1173:1177	white blood cells	1161:1177	white blood cells	1161:1177	In vivo study showed a decrease in red blood cells (70%) and platelet counts (46%), hemoglobin content (8%), hematocrit percent (7%), and an 80% increase of white blood cells in Ba-treated rats.
26971618	0	43	theme	chromosomal	63:73	arg1	aberrations					75:85	chromosomal aberrations	63:85	chromosomal aberrations	63:85	Protective effects of pomegranate peel against hematotoxicity, chromosomal aberrations, and genotoxicity induced by barium chloride in adult rats.
26971618	7	44	theme	peroxidase	1256:1265	arg1	activities					1267:1276	glutathione peroxidase activities	1244:1276	glutathione peroxidase activities	1244:1276	A reduction in antioxidant status: catalase, glutathione peroxidase activities, glutathione, and vitamin C levels by 31, 21, 28, and 29%, respectively, and an increase in MDA (46%) and AOPP levels (72%) were also observed compared with controls.
26971618	5	45	dep	content	969:975	arg1	315 ± 5 mg/g					978:989	315 ± 5 mg/g	978:989	315 ± 5 mg/g of extract	978:1000	DPPH test found IC50 value= 5.3 μg/mL and a high polysaccharides content (315 ± 5 mg/g of extract).
26971618	3	46	theme	Wistar	493:498	arg1	rats					500:503	Adult Wistar rats	487:503	Adult Wistar rats	487:503	MATERIALS AND METHODS Adult Wistar rats were divided into four groups of six each: control, barium (67 ppm via drinking water), PP (5% via diet), and their combination during 21 d. Oxidative stress was determined by MDA, AOPP, and antioxidant status: CAT, GPx, GSH, Vit C. Osmotic fragility (OF), chromosomal aberrations (CAs), and micronucleus (MN) assays were also studied.
26971618	1	47	theme	CONTEXT	147:153	arg1	PP					173:174	PP	173:174	PP	173:174	CONTEXT Pomegranate peel (PP) has health benefits including antibacterial, antioxidant, anti-inflammatory, and antimutagenic properties.
26971618	1	47	theme	CONTEXT	147:153	arg1	peel					167:170	CONTEXT Pomegranate peel	147:170	CONTEXT Pomegranate peel (PP)	147:175	CONTEXT Pomegranate peel (PP) has health benefits including antibacterial, antioxidant, anti-inflammatory, and antimutagenic properties.
26971618	3	48	theme	chromosomal	762:772	arg1	CAs					787:789	CAs	787:789	CAs	787:789	MATERIALS AND METHODS Adult Wistar rats were divided into four groups of six each: control, barium (67 ppm via drinking water), PP (5% via diet), and their combination during 21 d. Oxidative stress was determined by MDA, AOPP, and antioxidant status: CAT, GPx, GSH, Vit C. Osmotic fragility (OF), chromosomal aberrations (CAs), and micronucleus (MN) assays were also studied.
26971618	3	48	theme	chromosomal	762:772	arg1	aberrations					774:784	chromosomal aberrations	762:784	chromosomal aberrations (CAs)	762:790	MATERIALS AND METHODS Adult Wistar rats were divided into four groups of six each: control, barium (67 ppm via drinking water), PP (5% via diet), and their combination during 21 d. Oxidative stress was determined by MDA, AOPP, and antioxidant status: CAT, GPx, GSH, Vit C. Osmotic fragility (OF), chromosomal aberrations (CAs), and micronucleus (MN) assays were also studied.
26971618	6	49	from	content	1099:1105	arg1	counts					1074:1079	platelet counts	1065:1079	platelet counts (46%)	1065:1085	In vivo study showed a decrease in red blood cells (70%) and platelet counts (46%), hemoglobin content (8%), hematocrit percent (7%), and an 80% increase of white blood cells in Ba-treated rats.
26971618	6	49	from	content	1099:1105	arg1	%					1084:1084	46%	1082:1084	46%	1082:1084	In vivo study showed a decrease in red blood cells (70%) and platelet counts (46%), hemoglobin content (8%), hematocrit percent (7%), and an 80% increase of white blood cells in Ba-treated rats.
26971618	6	49	from	content	1099:1105	arg1	cells					1049:1053	red blood cells	1039:1053	red blood cells (70%)	1039:1059	In vivo study showed a decrease in red blood cells (70%) and platelet counts (46%), hemoglobin content (8%), hematocrit percent (7%), and an 80% increase of white blood cells in Ba-treated rats.
26971618	6	49	from	content	1099:1105	arg1	%					1058:1058	70%	1056:1058	70%	1056:1058	In vivo study showed a decrease in red blood cells (70%) and platelet counts (46%), hemoglobin content (8%), hematocrit percent (7%), and an 80% increase of white blood cells in Ba-treated rats.
26971618	6	49	from	content	1099:1105	arg1	rats					1193:1196	Ba-treated rats	1182:1196	Ba-treated rats	1182:1196	In vivo study showed a decrease in red blood cells (70%) and platelet counts (46%), hemoglobin content (8%), hematocrit percent (7%), and an 80% increase of white blood cells in Ba-treated rats.
26971618	3	50	theme	Oxidative	646:654	arg1	stress					656:661	21 d. Oxidative stress	640:661	21 d. Oxidative stress	640:661	MATERIALS AND METHODS Adult Wistar rats were divided into four groups of six each: control, barium (67 ppm via drinking water), PP (5% via diet), and their combination during 21 d. Oxidative stress was determined by MDA, AOPP, and antioxidant status: CAT, GPx, GSH, Vit C. Osmotic fragility (OF), chromosomal aberrations (CAs), and micronucleus (MN) assays were also studied.
26971618	8	51	theme	chromosomal	1519:1529	arg1	aberrations					1531:1541	total chromosomal aberrations	1513:1541	total chromosomal aberrations with abnormal metaphases	1513:1566	BaCl2-treatment showed a significant increase in the frequencies of total chromosomal aberrations with abnormal metaphases and micronucleus in bone-marrow cells.
26971618	7	52	theme	antioxidant	1214:1224	arg1	status					1226:1231	antioxidant status	1214:1231	antioxidant status	1214:1231	A reduction in antioxidant status: catalase, glutathione peroxidase activities, glutathione, and vitamin C levels by 31, 21, 28, and 29%, respectively, and an increase in MDA (46%) and AOPP levels (72%) were also observed compared with controls.
26971618	7	53	dep	observed	1412:1419	arg1	compared					1421:1428	compared	1421:1428	compared with controls	1421:1442	A reduction in antioxidant status: catalase, glutathione peroxidase activities, glutathione, and vitamin C levels by 31, 21, 28, and 29%, respectively, and an increase in MDA (46%) and AOPP levels (72%) were also observed compared with controls.
26971618	1	54	theme	antimutagenic	258:270	arg1	properties					272:281	antibacterial, antioxidant, anti-inflammatory, and antimutagenic properties	207:281	antibacterial, antioxidant, anti-inflammatory, and antimutagenic properties	207:281	CONTEXT Pomegranate peel (PP) has health benefits including antibacterial, antioxidant, anti-inflammatory, and antimutagenic properties.
26971618	3	55	theme	six	538:540	arg1	six					538:540	six	538:540	six	538:540	MATERIALS AND METHODS Adult Wistar rats were divided into four groups of six each: control, barium (67 ppm via drinking water), PP (5% via diet), and their combination during 21 d. Oxidative stress was determined by MDA, AOPP, and antioxidant status: CAT, GPx, GSH, Vit C. Osmotic fragility (OF), chromosomal aberrations (CAs), and micronucleus (MN) assays were also studied.
26971618	3	55	theme	six	538:540	arg1	groups					528:533	four groups	523:533	four groups of six each	523:545	MATERIALS AND METHODS Adult Wistar rats were divided into four groups of six each: control, barium (67 ppm via drinking water), PP (5% via diet), and their combination during 21 d. Oxidative stress was determined by MDA, AOPP, and antioxidant status: CAT, GPx, GSH, Vit C. Osmotic fragility (OF), chromosomal aberrations (CAs), and micronucleus (MN) assays were also studied.
26971618	6	56	dep	In	1004:1005	arg1	vivo					1007:1010	vivo	1007:1010	vivo	1007:1010	In vivo study showed a decrease in red blood cells (70%) and platelet counts (46%), hemoglobin content (8%), hematocrit percent (7%), and an 80% increase of white blood cells in Ba-treated rats.
26971618	9	57	theme	Oxidative	1607:1615	arg1	stress					1617:1622	Oxidative stress	1607:1622	Oxidative stress induced by BaCl2	1607:1639	Oxidative stress induced by BaCl2 might be the major cause for chromosomal abnormalities leading to DNA damage.
26971618	9	57	theme	Oxidative	1607:1615	arg1	cause					1660:1664	the major cause	1650:1664	the major cause for chromosomal abnormalities leading to DNA damage	1650:1716	Oxidative stress induced by BaCl2 might be the major cause for chromosomal abnormalities leading to DNA damage.
26971618	8	58	theme	bone-marrow	1588:1598	arg1	cells					1600:1604	bone-marrow cells	1588:1604	bone-marrow cells	1588:1604	BaCl2-treatment showed a significant increase in the frequencies of total chromosomal aberrations with abnormal metaphases and micronucleus in bone-marrow cells.
26971618	1	59	theme	health	181:186	arg1	benefits					188:195	health benefits	181:195	health benefits	181:195	CONTEXT Pomegranate peel (PP) has health benefits including antibacterial, antioxidant, anti-inflammatory, and antimutagenic properties.
26971618	6	60	theme	In	1004:1005	arg1	study					1012:1016	In vivo study	1004:1016	In vivo study	1004:1016	In vivo study showed a decrease in red blood cells (70%) and platelet counts (46%), hemoglobin content (8%), hematocrit percent (7%), and an 80% increase of white blood cells in Ba-treated rats.
26971618	10	61	theme	powerful	1821:1828	arg1	capacity					1842:1849	its powerful antioxidant capacity	1817:1849	its powerful antioxidant capacity	1817:1849	DISCUSSION AND CONCLUSION A decrease in hematotoxic and genotoxic effects induced by PP is due to its powerful antioxidant capacity.
26971618	6	62	theme	hematocrit	1113:1122	arg1	percent					1124:1130	hematocrit percent	1113:1130	hematocrit percent (7%)	1113:1135	In vivo study showed a decrease in red blood cells (70%) and platelet counts (46%), hemoglobin content (8%), hematocrit percent (7%), and an 80% increase of white blood cells in Ba-treated rats.
26971618	6	62	theme	hematocrit	1113:1122	arg1	%					1134:1134	7%	1133:1134	7%	1133:1134	In vivo study showed a decrease in red blood cells (70%) and platelet counts (46%), hemoglobin content (8%), hematocrit percent (7%), and an 80% increase of white blood cells in Ba-treated rats.
26971618	5	63	theme	high	948:951	arg1	content					969:975	a high polysaccharides content	946:975	a high polysaccharides content (315 ± 5 mg/g of extract)	946:1001	DPPH test found IC50 value= 5.3 μg/mL and a high polysaccharides content (315 ± 5 mg/g of extract).
26971618	3	64	dep	six	538:540	arg1	each					542:545	each	542:545	each	542:545	MATERIALS AND METHODS Adult Wistar rats were divided into four groups of six each: control, barium (67 ppm via drinking water), PP (5% via diet), and their combination during 21 d. Oxidative stress was determined by MDA, AOPP, and antioxidant status: CAT, GPx, GSH, Vit C. Osmotic fragility (OF), chromosomal aberrations (CAs), and micronucleus (MN) assays were also studied.
26971618	3	65	theme	antioxidant	696:706	arg1	status					708:713	antioxidant status	696:713	antioxidant status	696:713	MATERIALS AND METHODS Adult Wistar rats were divided into four groups of six each: control, barium (67 ppm via drinking water), PP (5% via diet), and their combination during 21 d. Oxidative stress was determined by MDA, AOPP, and antioxidant status: CAT, GPx, GSH, Vit C. Osmotic fragility (OF), chromosomal aberrations (CAs), and micronucleus (MN) assays were also studied.
26971618	6	66	from	increase	1149:1156	arg1	counts					1074:1079	platelet counts	1065:1079	platelet counts (46%)	1065:1085	In vivo study showed a decrease in red blood cells (70%) and platelet counts (46%), hemoglobin content (8%), hematocrit percent (7%), and an 80% increase of white blood cells in Ba-treated rats.
26971618	6	66	from	increase	1149:1156	arg1	%					1084:1084	46%	1082:1084	46%	1082:1084	In vivo study showed a decrease in red blood cells (70%) and platelet counts (46%), hemoglobin content (8%), hematocrit percent (7%), and an 80% increase of white blood cells in Ba-treated rats.
26971618	6	66	from	increase	1149:1156	arg1	cells					1049:1053	red blood cells	1039:1053	red blood cells (70%)	1039:1059	In vivo study showed a decrease in red blood cells (70%) and platelet counts (46%), hemoglobin content (8%), hematocrit percent (7%), and an 80% increase of white blood cells in Ba-treated rats.
26971618	6	66	from	increase	1149:1156	arg1	%					1058:1058	70%	1056:1058	70%	1056:1058	In vivo study showed a decrease in red blood cells (70%) and platelet counts (46%), hemoglobin content (8%), hematocrit percent (7%), and an 80% increase of white blood cells in Ba-treated rats.
26971618	6	66	from	increase	1149:1156	arg1	rats					1193:1196	Ba-treated rats	1182:1196	Ba-treated rats	1182:1196	In vivo study showed a decrease in red blood cells (70%) and platelet counts (46%), hemoglobin content (8%), hematocrit percent (7%), and an 80% increase of white blood cells in Ba-treated rats.
26971618	9	67	theme	major	1654:1658	arg1	cause					1660:1664	the major cause	1650:1664	the major cause for chromosomal abnormalities leading to DNA damage	1650:1716	Oxidative stress induced by BaCl2 might be the major cause for chromosomal abnormalities leading to DNA damage.
26971618	9	67	theme	major	1654:1658	arg1	stress					1617:1622	Oxidative stress	1607:1622	Oxidative stress induced by BaCl2	1607:1639	Oxidative stress induced by BaCl2 might be the major cause for chromosomal abnormalities leading to DNA damage.
26971618	2	68	theme	protective	350:359	arg1	effects					361:367	protective effects	350:367	protective effects	350:367	OBJECTIVE This study investigated the biochemical composition and protective effects of PP against hematotoxicity and genotoxicity induced by barium chloride (BaCl2) in adult rats.
26971618	6	69	theme	%	1147:1147	arg1	increase					1149:1156	an 80% increase	1142:1156	an 80% increase of white blood cells in Ba-treated rats	1142:1196	In vivo study showed a decrease in red blood cells (70%) and platelet counts (46%), hemoglobin content (8%), hematocrit percent (7%), and an 80% increase of white blood cells in Ba-treated rats.
26971618	5	70	theme	IC50	920:923	arg1	value= 5.3 μg/mL					925:940	IC50 value= 5.3 μg/mL	920:940	IC50 value= 5.3 μg/mL	920:940	DPPH test found IC50 value= 5.3 μg/mL and a high polysaccharides content (315 ± 5 mg/g of extract).
26971618	3	71	theme	fragility	746:754	arg1	assays					815:820	CAT, GPx, GSH, Vit C. Osmotic fragility (OF), chromosomal aberrations (CAs), and micronucleus (MN) assays	716:820	CAT, GPx, GSH, Vit C. Osmotic fragility (OF), chromosomal aberrations (CAs), and micronucleus (MN) assays	716:820	MATERIALS AND METHODS Adult Wistar rats were divided into four groups of six each: control, barium (67 ppm via drinking water), PP (5% via diet), and their combination during 21 d. Oxidative stress was determined by MDA, AOPP, and antioxidant status: CAT, GPx, GSH, Vit C. Osmotic fragility (OF), chromosomal aberrations (CAs), and micronucleus (MN) assays were also studied.
26971618	6	72	theme	blood	1043:1047	arg1	cells					1049:1053	red blood cells	1039:1053	red blood cells (70%)	1039:1059	In vivo study showed a decrease in red blood cells (70%) and platelet counts (46%), hemoglobin content (8%), hematocrit percent (7%), and an 80% increase of white blood cells in Ba-treated rats.
26971618	6	72	theme	blood	1043:1047	arg1	%					1058:1058	70%	1056:1058	70%	1056:1058	In vivo study showed a decrease in red blood cells (70%) and platelet counts (46%), hemoglobin content (8%), hematocrit percent (7%), and an 80% increase of white blood cells in Ba-treated rats.
26971618	5	73	theme	extract	994:1000	arg1	315 ± 5 mg/g					978:989	315 ± 5 mg/g	978:989	315 ± 5 mg/g of extract	978:1000	DPPH test found IC50 value= 5.3 μg/mL and a high polysaccharides content (315 ± 5 mg/g of extract).
26971618	1	74	theme	Pomegranate	155:165	arg1	PP					173:174	PP	173:174	PP	173:174	CONTEXT Pomegranate peel (PP) has health benefits including antibacterial, antioxidant, anti-inflammatory, and antimutagenic properties.
26971618	1	74	theme	Pomegranate	155:165	arg1	peel					167:170	CONTEXT Pomegranate peel	147:170	CONTEXT Pomegranate peel (PP)	147:175	CONTEXT Pomegranate peel (PP) has health benefits including antibacterial, antioxidant, anti-inflammatory, and antimutagenic properties.
26971618	3	75	theme	aberrations	774:784	arg1	assays					815:820	CAT, GPx, GSH, Vit C. Osmotic fragility (OF), chromosomal aberrations (CAs), and micronucleus (MN) assays	716:820	CAT, GPx, GSH, Vit C. Osmotic fragility (OF), chromosomal aberrations (CAs), and micronucleus (MN) assays	716:820	MATERIALS AND METHODS Adult Wistar rats were divided into four groups of six each: control, barium (67 ppm via drinking water), PP (5% via diet), and their combination during 21 d. Oxidative stress was determined by MDA, AOPP, and antioxidant status: CAT, GPx, GSH, Vit C. Osmotic fragility (OF), chromosomal aberrations (CAs), and micronucleus (MN) assays were also studied.
26971618	3	76	theme	C.	735:736	arg1	fragility					746:754	Vit C. Osmotic fragility	731:754	Vit C. Osmotic fragility (OF)	731:759	MATERIALS AND METHODS Adult Wistar rats were divided into four groups of six each: control, barium (67 ppm via drinking water), PP (5% via diet), and their combination during 21 d. Oxidative stress was determined by MDA, AOPP, and antioxidant status: CAT, GPx, GSH, Vit C. Osmotic fragility (OF), chromosomal aberrations (CAs), and micronucleus (MN) assays were also studied.
26971618	3	77	dep	fragility	746:754	arg1	OF					757:758	OF	757:758	OF	757:758	MATERIALS AND METHODS Adult Wistar rats were divided into four groups of six each: control, barium (67 ppm via drinking water), PP (5% via diet), and their combination during 21 d. Oxidative stress was determined by MDA, AOPP, and antioxidant status: CAT, GPx, GSH, Vit C. Osmotic fragility (OF), chromosomal aberrations (CAs), and micronucleus (MN) assays were also studied.
26971618	7	78	theme	vitamin	1296:1302	arg1	levels					1306:1311	vitamin C levels	1296:1311	vitamin C levels	1296:1311	A reduction in antioxidant status: catalase, glutathione peroxidase activities, glutathione, and vitamin C levels by 31, 21, 28, and 29%, respectively, and an increase in MDA (46%) and AOPP levels (72%) were also observed compared with controls.
26971618	6	79	from	decrease	1027:1034	arg1	counts					1074:1079	platelet counts	1065:1079	platelet counts (46%)	1065:1085	In vivo study showed a decrease in red blood cells (70%) and platelet counts (46%), hemoglobin content (8%), hematocrit percent (7%), and an 80% increase of white blood cells in Ba-treated rats.
26971618	6	79	from	decrease	1027:1034	arg1	%					1084:1084	46%	1082:1084	46%	1082:1084	In vivo study showed a decrease in red blood cells (70%) and platelet counts (46%), hemoglobin content (8%), hematocrit percent (7%), and an 80% increase of white blood cells in Ba-treated rats.
26971618	6	79	from	decrease	1027:1034	arg1	cells					1049:1053	red blood cells	1039:1053	red blood cells (70%)	1039:1059	In vivo study showed a decrease in red blood cells (70%) and platelet counts (46%), hemoglobin content (8%), hematocrit percent (7%), and an 80% increase of white blood cells in Ba-treated rats.
26971618	6	79	from	decrease	1027:1034	arg1	%					1058:1058	70%	1056:1058	70%	1056:1058	In vivo study showed a decrease in red blood cells (70%) and platelet counts (46%), hemoglobin content (8%), hematocrit percent (7%), and an 80% increase of white blood cells in Ba-treated rats.
26971618	6	79	from	decrease	1027:1034	arg1	rats					1193:1196	Ba-treated rats	1182:1196	Ba-treated rats	1182:1196	In vivo study showed a decrease in red blood cells (70%) and platelet counts (46%), hemoglobin content (8%), hematocrit percent (7%), and an 80% increase of white blood cells in Ba-treated rats.
26971618	0	80	theme	barium	116:121	arg1	chloride					123:130	barium chloride	116:130	barium chloride	116:130	Protective effects of pomegranate peel against hematotoxicity, chromosomal aberrations, and genotoxicity induced by barium chloride in adult rats.
26971618	8	81	theme	significant	1470:1480	arg1	increase					1482:1489	a significant increase	1468:1489	a significant increase in the frequencies of total chromosomal aberrations with abnormal metaphases	1468:1566	BaCl2-treatment showed a significant increase in the frequencies of total chromosomal aberrations with abnormal metaphases and micronucleus in bone-marrow cells.
26971618	10	82	from	decrease	1747:1754	arg1	effects					1785:1791	hematotoxic and genotoxic effects	1759:1791	hematotoxic and genotoxic effects induced by PP	1759:1805	DISCUSSION AND CONCLUSION A decrease in hematotoxic and genotoxic effects induced by PP is due to its powerful antioxidant capacity.
26971618	0	83	theme	pomegranate	22:32	arg1	effects					11:17	Protective effects	0:17	Protective effects of pomegranate	0:32	Protective effects of pomegranate peel against hematotoxicity, chromosomal aberrations, and genotoxicity induced by barium chloride in adult rats.
26971618	4	84	theme	compounds	893:901	arg1	composition					866:876	a rich composition	859:876	a rich composition of antioxidant compounds	859:901	RESULTS PP showed a rich composition of antioxidant compounds.
26971618	1	85	contain	has	177:179	arg2	benefits					188:195	health benefits	181:195	health benefits	181:195	CONTEXT Pomegranate peel (PP) has health benefits including antibacterial, antioxidant, anti-inflammatory, and antimutagenic properties.
26971618	1	85	contain	has	177:179	arg1	PP					173:174	PP	173:174	PP	173:174	CONTEXT Pomegranate peel (PP) has health benefits including antibacterial, antioxidant, anti-inflammatory, and antimutagenic properties.
26971618	1	85	contain	has	177:179	arg1	peel					167:170	CONTEXT Pomegranate peel	147:170	CONTEXT Pomegranate peel (PP)	147:175	CONTEXT Pomegranate peel (PP) has health benefits including antibacterial, antioxidant, anti-inflammatory, and antimutagenic properties.
26971618	3	86	theme	Adult	487:491	arg1	rats					500:503	Adult Wistar rats	487:503	Adult Wistar rats	487:503	MATERIALS AND METHODS Adult Wistar rats were divided into four groups of six each: control, barium (67 ppm via drinking water), PP (5% via diet), and their combination during 21 d. Oxidative stress was determined by MDA, AOPP, and antioxidant status: CAT, GPx, GSH, Vit C. Osmotic fragility (OF), chromosomal aberrations (CAs), and micronucleus (MN) assays were also studied.
26971618	6	87	theme	blood	1167:1171	arg1	cells					1173:1177	white blood cells	1161:1177	white blood cells	1161:1177	In vivo study showed a decrease in red blood cells (70%) and platelet counts (46%), hemoglobin content (8%), hematocrit percent (7%), and an 80% increase of white blood cells in Ba-treated rats.
26971618	6	88	from	percent	1124:1130	arg1	counts					1074:1079	platelet counts	1065:1079	platelet counts (46%)	1065:1085	In vivo study showed a decrease in red blood cells (70%) and platelet counts (46%), hemoglobin content (8%), hematocrit percent (7%), and an 80% increase of white blood cells in Ba-treated rats.
26971618	6	88	from	percent	1124:1130	arg1	%					1084:1084	46%	1082:1084	46%	1082:1084	In vivo study showed a decrease in red blood cells (70%) and platelet counts (46%), hemoglobin content (8%), hematocrit percent (7%), and an 80% increase of white blood cells in Ba-treated rats.
26971618	6	88	from	percent	1124:1130	arg1	cells					1049:1053	red blood cells	1039:1053	red blood cells (70%)	1039:1059	In vivo study showed a decrease in red blood cells (70%) and platelet counts (46%), hemoglobin content (8%), hematocrit percent (7%), and an 80% increase of white blood cells in Ba-treated rats.
26971618	6	88	from	percent	1124:1130	arg1	%					1058:1058	70%	1056:1058	70%	1056:1058	In vivo study showed a decrease in red blood cells (70%) and platelet counts (46%), hemoglobin content (8%), hematocrit percent (7%), and an 80% increase of white blood cells in Ba-treated rats.
26971618	6	88	from	percent	1124:1130	arg1	rats					1193:1196	Ba-treated rats	1182:1196	Ba-treated rats	1182:1196	In vivo study showed a decrease in red blood cells (70%) and platelet counts (46%), hemoglobin content (8%), hematocrit percent (7%), and an 80% increase of white blood cells in Ba-treated rats.
26971618	3	89	theme	micronucleus	797:808	arg1	assays					815:820	CAT, GPx, GSH, Vit C. Osmotic fragility (OF), chromosomal aberrations (CAs), and micronucleus (MN) assays	716:820	CAT, GPx, GSH, Vit C. Osmotic fragility (OF), chromosomal aberrations (CAs), and micronucleus (MN) assays	716:820	MATERIALS AND METHODS Adult Wistar rats were divided into four groups of six each: control, barium (67 ppm via drinking water), PP (5% via diet), and their combination during 21 d. Oxidative stress was determined by MDA, AOPP, and antioxidant status: CAT, GPx, GSH, Vit C. Osmotic fragility (OF), chromosomal aberrations (CAs), and micronucleus (MN) assays were also studied.
26971618	8	90	from	micronucleus	1572:1583	arg1	cells					1600:1604	bone-marrow cells	1588:1604	bone-marrow cells	1588:1604	BaCl2-treatment showed a significant increase in the frequencies of total chromosomal aberrations with abnormal metaphases and micronucleus in bone-marrow cells.
26971618	8	90	from	micronucleus	1572:1583	arg1	frequencies					1498:1508	the frequencies	1494:1508	the frequencies of total chromosomal aberrations with abnormal metaphases	1494:1566	BaCl2-treatment showed a significant increase in the frequencies of total chromosomal aberrations with abnormal metaphases and micronucleus in bone-marrow cells.
26971618	4	91	theme	rich	861:864	arg1	composition					866:876	a rich composition	859:876	a rich composition of antioxidant compounds	859:901	RESULTS PP showed a rich composition of antioxidant compounds.
26971618	3	92	dep	MATERIALS	465:473	arg1	divided					510:516	divided	510:516	were divided into four groups of six each	505:545	MATERIALS AND METHODS Adult Wistar rats were divided into four groups of six each: control, barium (67 ppm via drinking water), PP (5% via diet), and their combination during 21 d. Oxidative stress was determined by MDA, AOPP, and antioxidant status: CAT, GPx, GSH, Vit C. Osmotic fragility (OF), chromosomal aberrations (CAs), and micronucleus (MN) assays were also studied.
26971618	3	92	dep	MATERIALS	465:473	arg1	determined					667:676	determined	667:676	was determined by MDA, AOPP, and antioxidant status	663:713	MATERIALS AND METHODS Adult Wistar rats were divided into four groups of six each: control, barium (67 ppm via drinking water), PP (5% via diet), and their combination during 21 d. Oxidative stress was determined by MDA, AOPP, and antioxidant status: CAT, GPx, GSH, Vit C. Osmotic fragility (OF), chromosomal aberrations (CAs), and micronucleus (MN) assays were also studied.
26971618	1	93	theme	antioxidant	222:232	arg1	properties					272:281	antibacterial, antioxidant, anti-inflammatory, and antimutagenic properties	207:281	antibacterial, antioxidant, anti-inflammatory, and antimutagenic properties	207:281	CONTEXT Pomegranate peel (PP) has health benefits including antibacterial, antioxidant, anti-inflammatory, and antimutagenic properties.
26971618	7	94	theme	glutathione	1244:1254	arg1	activities					1267:1276	glutathione peroxidase activities	1244:1276	glutathione peroxidase activities	1244:1276	A reduction in antioxidant status: catalase, glutathione peroxidase activities, glutathione, and vitamin C levels by 31, 21, 28, and 29%, respectively, and an increase in MDA (46%) and AOPP levels (72%) were also observed compared with controls.
26971618	3	95	theme	d.	643:644	arg1	stress					656:661	21 d. Oxidative stress	640:661	21 d. Oxidative stress	640:661	MATERIALS AND METHODS Adult Wistar rats were divided into four groups of six each: control, barium (67 ppm via drinking water), PP (5% via diet), and their combination during 21 d. Oxidative stress was determined by MDA, AOPP, and antioxidant status: CAT, GPx, GSH, Vit C. Osmotic fragility (OF), chromosomal aberrations (CAs), and micronucleus (MN) assays were also studied.
26971618	1	96	theme	anti-inflammatory	235:251	arg1	properties					272:281	antibacterial, antioxidant, anti-inflammatory, and antimutagenic properties	207:281	antibacterial, antioxidant, anti-inflammatory, and antimutagenic properties	207:281	CONTEXT Pomegranate peel (PP) has health benefits including antibacterial, antioxidant, anti-inflammatory, and antimutagenic properties.
28288881	0	0	theme	thermal	80:86	arg1	properties					103:112	Physical, thermal and structural properties	70:112	cassava starch hybrid composites: Physical, thermal and structural properties	36:112	Cassava/sugar palm fiber reinforced cassava starch hybrid composites: Physical, thermal and structural properties.
28288881	2	1	theme	CS/CB	369:373	arg1	film					385:388	the CS/CB composite film	365:388	the CS/CB composite film containing 6% CB	365:405	Different loadings of SPF (2, 4, 6 and 8% w/w of dry starch) were added to the CS/CB composite film containing 6% CB.
28288881	5	2	theme	thermal	639:645	arg1	properties					647:656	the thermal properties	635:656	the thermal properties of the hybrid composite film	635:685	However, no significant effect was noticed on the thermal properties of the hybrid composite film.
28288881	8	3	theme	heterogeneous	938:950	arg1	surface					952:958	a more heterogeneous surface	931:958	a more heterogeneous surface	931:958	The film with a higher concentration of SPF (CS-CB/SPF8) showed a more heterogeneous surface.
28288881	1	4	theme	cassava	152:158	arg1	CB					169:170	CB	169:170	CB	169:170	A hybrid composite was prepared from cassava bagasse (CB) and sugar palm fiber (SPF) using casting technique with cassava starch (CS) as matrix and fructose as a plasticizer.
28288881	1	4	theme	cassava	152:158	arg1	bagasse					160:166	cassava bagasse	152:166	cassava bagasse (CB)	152:171	A hybrid composite was prepared from cassava bagasse (CB) and sugar palm fiber (SPF) using casting technique with cassava starch (CS) as matrix and fructose as a plasticizer.
28288881	9	5	theme	film	1089:1092	arg1	performances					1094:1105	the film performances	1085:1105	the film performances	1085:1105	It could be concluded that the incorporation of SPF led to changes in cassava starch film properties, potentially affecting the film performances.
28288881	4	6	theme	water	571:575	arg1	absorption					577:586	water absorption	571:586	water absorption	571:586	It increased the thickness while decreasing the density, water content, water solubility and water absorption.
28288881	0	7	theme	structural	92:101	arg1	properties					103:112	Physical, thermal and structural properties	70:112	cassava starch hybrid composites: Physical, thermal and structural properties	36:112	Cassava/sugar palm fiber reinforced cassava starch hybrid composites: Physical, thermal and structural properties.
28288881	3	8	theme	physical	457:464	arg1	properties					466:475	the physical properties	453:475	the physical properties	453:475	The addition of SPF significantly influenced the physical properties.
28288881	1	9	theme	cassava	229:235	arg1	CS					245:246	CS	245:246	CS	245:246	A hybrid composite was prepared from cassava bagasse (CB) and sugar palm fiber (SPF) using casting technique with cassava starch (CS) as matrix and fructose as a plasticizer.
28288881	1	9	theme	cassava	229:235	arg1	starch					237:242	cassava starch	229:242	cassava starch (CS)	229:247	A hybrid composite was prepared from cassava bagasse (CB) and sugar palm fiber (SPF) using casting technique with cassava starch (CS) as matrix and fructose as a plasticizer.
28288881	2	10	theme	%	402:402	arg1	CB					404:405	6% CB	401:405	6% CB	401:405	Different loadings of SPF (2, 4, 6 and 8% w/w of dry starch) were added to the CS/CB composite film containing 6% CB.
28288881	7	11	theme	SEM	793:795	arg1	micrographs					797:807	SEM micrographs	793:807	SEM micrographs	793:807	SEM micrographs indicated that the filler was incorporated in the matrix.
28288881	2	12	theme	6	401:401	arg1	%					402:402	%	402:402	%	402:402	Different loadings of SPF (2, 4, 6 and 8% w/w of dry starch) were added to the CS/CB composite film containing 6% CB.
28288881	5	13	theme	film	682:685	arg1	properties					647:656	the thermal properties	635:656	the thermal properties of the hybrid composite film	635:685	However, no significant effect was noticed on the thermal properties of the hybrid composite film.
28288881	5	14	theme	composite	672:680	arg1	film					682:685	the hybrid composite film	661:685	the hybrid composite film	661:685	However, no significant effect was noticed on the thermal properties of the hybrid composite film.
28288881	8	15	theme	higher	883:888	arg1	concentration					890:902	a higher concentration	881:902	a higher concentration of SPF (CS-CB/SPF8)	881:922	The film with a higher concentration of SPF (CS-CB/SPF8) showed a more heterogeneous surface.
28288881	2	16	theme	dry	339:341	arg1	starch					343:348	dry starch	339:348	dry starch	339:348	Different loadings of SPF (2, 4, 6 and 8% w/w of dry starch) were added to the CS/CB composite film containing 6% CB.
28288881	8	17	with	film	871:874	arg1	concentration					890:902	a higher concentration	881:902	a higher concentration of SPF (CS-CB/SPF8)	881:922	The film with a higher concentration of SPF (CS-CB/SPF8) showed a more heterogeneous surface.
28288881	4	18	theme	water	535:539	arg1	content					541:547	water content	535:547	water content	535:547	It increased the thickness while decreasing the density, water content, water solubility and water absorption.
28288881	2	19	theme	starch	343:348	arg1	starch					343:348	dry starch	339:348	dry starch	339:348	Different loadings of SPF (2, 4, 6 and 8% w/w of dry starch) were added to the CS/CB composite film containing 6% CB.
28288881	2	19	theme	starch	343:348	arg1	%					330:330	2, 4, 6 and 8%	317:330	2, 4, 6 and 8% w/w of dry starch	317:348	Different loadings of SPF (2, 4, 6 and 8% w/w of dry starch) were added to the CS/CB composite film containing 6% CB.
28288881	8	20	theme	SPF	907:909	arg1	concentration					890:902	a higher concentration	881:902	a higher concentration of SPF (CS-CB/SPF8)	881:922	The film with a higher concentration of SPF (CS-CB/SPF8) showed a more heterogeneous surface.
28288881	4	21	theme	water	550:554	arg1	solubility					556:565	water solubility	550:565	water solubility	550:565	It increased the thickness while decreasing the density, water content, water solubility and water absorption.
28288881	0	22	theme	Cassava/sugar	0:12	arg1	fiber					19:23	Cassava/sugar palm fiber	0:23	Cassava/sugar palm fiber	0:23	Cassava/sugar palm fiber reinforced cassava starch hybrid composites: Physical, thermal and structural properties.
28288881	9	23	theme	cassava	1031:1037	arg1	properties					1051:1060	cassava starch film properties	1031:1060	cassava starch film properties	1031:1060	It could be concluded that the incorporation of SPF led to changes in cassava starch film properties, potentially affecting the film performances.
28288881	9	24	theme	starch	1039:1044	arg1	properties					1051:1060	cassava starch film properties	1031:1060	cassava starch film properties	1031:1060	It could be concluded that the incorporation of SPF led to changes in cassava starch film properties, potentially affecting the film performances.
28288881	3	25	theme	SPF	424:426	arg1	addition					412:419	The addition	408:419	The addition of SPF	408:426	The addition of SPF significantly influenced the physical properties.
28288881	1	26	theme	sugar	177:181	arg1	SPF					195:197	SPF	195:197	SPF	195:197	A hybrid composite was prepared from cassava bagasse (CB) and sugar palm fiber (SPF) using casting technique with cassava starch (CS) as matrix and fructose as a plasticizer.
28288881	1	26	theme	sugar	177:181	arg1	fiber					188:192	sugar palm fiber	177:192	sugar palm fiber (SPF)	177:198	A hybrid composite was prepared from cassava bagasse (CB) and sugar palm fiber (SPF) using casting technique with cassava starch (CS) as matrix and fructose as a plasticizer.
28288881	9	27	theme	film	1046:1049	arg1	properties					1051:1060	cassava starch film properties	1031:1060	cassava starch film properties	1031:1060	It could be concluded that the incorporation of SPF led to changes in cassava starch film properties, potentially affecting the film performances.
28288881	1	28	theme	palm	183:186	arg1	SPF					195:197	SPF	195:197	SPF	195:197	A hybrid composite was prepared from cassava bagasse (CB) and sugar palm fiber (SPF) using casting technique with cassava starch (CS) as matrix and fructose as a plasticizer.
28288881	1	28	theme	palm	183:186	arg1	fiber					188:192	sugar palm fiber	177:192	sugar palm fiber (SPF)	177:198	A hybrid composite was prepared from cassava bagasse (CB) and sugar palm fiber (SPF) using casting technique with cassava starch (CS) as matrix and fructose as a plasticizer.
28288881	0	29	theme	starch	44:49	arg1	composites					58:67	cassava starch hybrid composites	36:67	cassava starch hybrid composites: Physical, thermal and structural properties	36:112	Cassava/sugar palm fiber reinforced cassava starch hybrid composites: Physical, thermal and structural properties.
28288881	0	30	dep	composites	58:67	arg1	properties					103:112	Physical, thermal and structural properties	70:112	cassava starch hybrid composites: Physical, thermal and structural properties	36:112	Cassava/sugar palm fiber reinforced cassava starch hybrid composites: Physical, thermal and structural properties.
28288881	0	31	theme	cassava	36:42	arg1	composites					58:67	cassava starch hybrid composites	36:67	cassava starch hybrid composites: Physical, thermal and structural properties	36:112	Cassava/sugar palm fiber reinforced cassava starch hybrid composites: Physical, thermal and structural properties.
28288881	6	32	dep	crystallinity	736:748	arg1	%					758:758	%	758:758	%	758:758	The incorporation of SPF increased the relative crystallinity up to 47%, compared to 32% of the CS film.
28288881	5	33	theme	hybrid	665:670	arg1	film					682:685	the hybrid composite film	661:685	the hybrid composite film	661:685	However, no significant effect was noticed on the thermal properties of the hybrid composite film.
28288881	5	34	theme	significant	601:611	arg1	effect					613:618	no significant effect	598:618	no significant effect	598:618	However, no significant effect was noticed on the thermal properties of the hybrid composite film.
28288881	6	35	theme	relative	727:734	arg1	crystallinity					736:748	the relative crystallinity	723:748	the relative crystallinity up to 47%	723:758	The incorporation of SPF increased the relative crystallinity up to 47%, compared to 32% of the CS film.
28288881	2	36	dep	%	330:330	arg1	w/w					332:334	w/w	332:334	2, 4, 6 and 8% w/w of dry starch	317:348	Different loadings of SPF (2, 4, 6 and 8% w/w of dry starch) were added to the CS/CB composite film containing 6% CB.
28288881	2	37	theme	Different	290:298	arg1	loadings					300:307	Different loadings	290:307	Different loadings of SPF (2, 4, 6 and 8% w/w of dry starch)	290:349	Different loadings of SPF (2, 4, 6 and 8% w/w of dry starch) were added to the CS/CB composite film containing 6% CB.
28288881	0	38	theme	hybrid	51:56	arg1	composites					58:67	cassava starch hybrid composites	36:67	cassava starch hybrid composites: Physical, thermal and structural properties	36:112	Cassava/sugar palm fiber reinforced cassava starch hybrid composites: Physical, thermal and structural properties.
28288881	6	39	theme	CS	784:785	arg1	film					787:790	the CS film	780:790	the CS film	780:790	The incorporation of SPF increased the relative crystallinity up to 47%, compared to 32% of the CS film.
28288881	1	40	with	technique	214:222	arg1	CS					245:246	CS	245:246	CS	245:246	A hybrid composite was prepared from cassava bagasse (CB) and sugar palm fiber (SPF) using casting technique with cassava starch (CS) as matrix and fructose as a plasticizer.
28288881	1	40	with	technique	214:222	arg1	starch					237:242	cassava starch	229:242	cassava starch (CS)	229:247	A hybrid composite was prepared from cassava bagasse (CB) and sugar palm fiber (SPF) using casting technique with cassava starch (CS) as matrix and fructose as a plasticizer.
28288881	1	41	theme	hybrid	117:122	arg1	composite					124:132	A hybrid composite	115:132	A hybrid composite	115:132	A hybrid composite was prepared from cassava bagasse (CB) and sugar palm fiber (SPF) using casting technique with cassava starch (CS) as matrix and fructose as a plasticizer.
28288881	9	42	theme	SPF	1009:1011	arg1	incorporation					992:1004	the incorporation	988:1004	the incorporation of SPF	988:1011	It could be concluded that the incorporation of SPF led to changes in cassava starch film properties, potentially affecting the film performances.
28288881	9	43	from	changes	1020:1026	arg1	properties					1051:1060	cassava starch film properties	1031:1060	cassava starch film properties	1031:1060	It could be concluded that the incorporation of SPF led to changes in cassava starch film properties, potentially affecting the film performances.
28288881	6	44	dep	%	758:758	arg1	47					756:757	47	756:757	47	756:757	The incorporation of SPF increased the relative crystallinity up to 47%, compared to 32% of the CS film.
28288881	2	45	dep	SPF	312:314	arg1	starch					343:348	dry starch	339:348	dry starch	339:348	Different loadings of SPF (2, 4, 6 and 8% w/w of dry starch) were added to the CS/CB composite film containing 6% CB.
28288881	2	45	dep	SPF	312:314	arg1	%					330:330	2, 4, 6 and 8%	317:330	2, 4, 6 and 8% w/w of dry starch	317:348	Different loadings of SPF (2, 4, 6 and 8% w/w of dry starch) were added to the CS/CB composite film containing 6% CB.
28288881	6	46	theme	SPF	709:711	arg1	incorporation					692:704	The incorporation	688:704	The incorporation of SPF	688:711	The incorporation of SPF increased the relative crystallinity up to 47%, compared to 32% of the CS film.
28288881	2	47	contain	containing	390:399	arg2	CB					404:405	6% CB	401:405	6% CB	401:405	Different loadings of SPF (2, 4, 6 and 8% w/w of dry starch) were added to the CS/CB composite film containing 6% CB.
28288881	2	47	contain	containing	390:399	arg1	film					385:388	the CS/CB composite film	365:388	the CS/CB composite film containing 6% CB	365:405	Different loadings of SPF (2, 4, 6 and 8% w/w of dry starch) were added to the CS/CB composite film containing 6% CB.
28288881	2	48	theme	SPF	312:314	arg1	loadings					300:307	Different loadings	290:307	Different loadings of SPF (2, 4, 6 and 8% w/w of dry starch)	290:349	Different loadings of SPF (2, 4, 6 and 8% w/w of dry starch) were added to the CS/CB composite film containing 6% CB.
28288881	6	49	dep	47	756:757	arg1	to					753:754	to	753:754	to	753:754	The incorporation of SPF increased the relative crystallinity up to 47%, compared to 32% of the CS film.
28288881	0	50	theme	Physical	70:77	arg1	properties					103:112	Physical, thermal and structural properties	70:112	cassava starch hybrid composites: Physical, thermal and structural properties	36:112	Cassava/sugar palm fiber reinforced cassava starch hybrid composites: Physical, thermal and structural properties.
28288881	2	51	theme	composite	375:383	arg1	film					385:388	the CS/CB composite film	365:388	the CS/CB composite film containing 6% CB	365:405	Different loadings of SPF (2, 4, 6 and 8% w/w of dry starch) were added to the CS/CB composite film containing 6% CB.
28288881	0	52	theme	palm	14:17	arg1	fiber					19:23	Cassava/sugar palm fiber	0:23	Cassava/sugar palm fiber	0:23	Cassava/sugar palm fiber reinforced cassava starch hybrid composites: Physical, thermal and structural properties.
28288881	6	53	theme	film	787:790	arg1	film					787:790	the CS film	780:790	the CS film	780:790	The incorporation of SPF increased the relative crystallinity up to 47%, compared to 32% of the CS film.
28288881	6	53	theme	film	787:790	arg1	%					775:775	32%	773:775	32% of the CS film	773:790	The incorporation of SPF increased the relative crystallinity up to 47%, compared to 32% of the CS film.
28288881	1	54	theme	casting	206:212	arg1	plasticizer					277:287	a plasticizer	275:287	a plasticizer	275:287	A hybrid composite was prepared from cassava bagasse (CB) and sugar palm fiber (SPF) using casting technique with cassava starch (CS) as matrix and fructose as a plasticizer.
28288881	1	54	theme	casting	206:212	arg1	technique					214:222	casting technique	206:222	casting technique with cassava starch (CS)	206:247	A hybrid composite was prepared from cassava bagasse (CB) and sugar palm fiber (SPF) using casting technique with cassava starch (CS) as matrix and fructose as a plasticizer.
25048214	0	0	theme	Streptomyces	86:97	arg1	sp					99:100	Streptomyces sp	86:100	Streptomyces sp	86:100	Characterization of an Ebosin derivative produced by heterologous gene replacement in Streptomyces sp.
25048214	4	1	theme	sugar	464:468	arg1	units					470:474	repeating sugar units	454:474	repeating sugar units	454:474	The Ebosin biosynthesis gene cluster (ste) consists of 27 ORFs and ste7 has previously been demonstrated to code for a fucosyltransferase, which plays an essential role in the formation of repeating sugar units during Ebosin production.
25048214	7	2	theme	binding	942:948	arg1	activity					950:957	a higher binding activity	933:957	a higher binding activity for IL-1R	933:967	In an ELISA analysis, EPS-7 g exhibited a higher binding activity for IL-1R, as a competitor of interleukin-1, than that of Ebosin.
25048214	7	2	theme	binding	942:948	arg1	competitor					975:984	a competitor	973:984	a competitor of interleukin-1	973:1001	In an ELISA analysis, EPS-7 g exhibited a higher binding activity for IL-1R, as a competitor of interleukin-1, than that of Ebosin.
25048214	8	3	from	activity	1077:1084	arg1	FLS					1170:1172	FLS	1170:1172	FLS	1170:1172	It also exhibited a higher inhibitory effect on the activity of IL-1β-converting enzyme and production of IL-1β in fibroblast-like synoviocytes (FLS).
25048214	8	3	from	activity	1077:1084	arg1	synoviocytes					1156:1167	fibroblast-like synoviocytes	1140:1167	fibroblast-like synoviocytes (FLS)	1140:1173	It also exhibited a higher inhibitory effect on the activity of IL-1β-converting enzyme and production of IL-1β in fibroblast-like synoviocytes (FLS).
25048214	4	4	theme	Ebosin	483:488	arg1	production					490:499	Ebosin production	483:499	Ebosin production	483:499	The Ebosin biosynthesis gene cluster (ste) consists of 27 ORFs and ste7 has previously been demonstrated to code for a fucosyltransferase, which plays an essential role in the formation of repeating sugar units during Ebosin production.
25048214	10	5	theme	Ebosin	1358:1363	arg1	g					1382:1382	The new Ebosin derivative EPS-7 g	1350:1382	The new Ebosin derivative EPS-7 g	1350:1382	CONCLUSIONS The new Ebosin derivative EPS-7 g is more bioactive than Ebosin evaluated by a series of experiments.
25048214	10	5	theme	Ebosin	1358:1363	arg1	bioactive					1392:1400	bioactive	1392:1400	bioactive	1392:1400	CONCLUSIONS The new Ebosin derivative EPS-7 g is more bioactive than Ebosin evaluated by a series of experiments.
25048214	4	6	theme	essential	419:427	arg1	role					429:432	an essential role	416:432	an essential role	416:432	The Ebosin biosynthesis gene cluster (ste) consists of 27 ORFs and ste7 has previously been demonstrated to code for a fucosyltransferase, which plays an essential role in the formation of repeating sugar units during Ebosin production.
25048214	8	7	theme	higher	1045:1050	arg1	effect					1063:1068	a higher inhibitory effect	1043:1068	a higher inhibitory effect on the activity of IL-1β-converting enzyme and production of IL-1β in fibroblast-like synoviocytes (FLS)	1043:1173	It also exhibited a higher inhibitory effect on the activity of IL-1β-converting enzyme and production of IL-1β in fibroblast-like synoviocytes (FLS).
25048214	2	8	theme	BACKGROUND	108:117	arg1	exopolysaccharide					137:153	a novel exopolysaccharide	129:153	a novel exopolysaccharide (EPS) produced by Streptomyces sp	129:187	BACKGROUND Ebosin is a novel exopolysaccharide (EPS) produced by Streptomyces sp.
25048214	2	8	theme	BACKGROUND	108:117	arg1	Ebosin					119:124	BACKGROUND Ebosin	108:124	BACKGROUND Ebosin	108:124	BACKGROUND Ebosin is a novel exopolysaccharide (EPS) produced by Streptomyces sp.
25048214	3	9	contain	possess	211:217	arg2	activity					247:254	an anti-rheumatic arthritis activity	219:254	an anti-rheumatic arthritis activity	219:254	139 and evidenced to possess an anti-rheumatic arthritis activity in vivo.
25048214	3	9	contain	possess	211:217	arg1	139					190:192	139	190:192	139	190:192	139 and evidenced to possess an anti-rheumatic arthritis activity in vivo.
25048214	11	10	theme	EPS	1509:1511	arg1	structure					1513:1521	EPS structure	1509:1521	EPS structure	1509:1521	This is the first report demonstrating a modification of EPS structure via heterologous gene replacement in Streptomyces.
25048214	8	11	from	production	1117:1126	arg1	FLS					1170:1172	FLS	1170:1172	FLS	1170:1172	It also exhibited a higher inhibitory effect on the activity of IL-1β-converting enzyme and production of IL-1β in fibroblast-like synoviocytes (FLS).
25048214	8	11	from	production	1117:1126	arg1	synoviocytes					1156:1167	fibroblast-like synoviocytes	1140:1167	fibroblast-like synoviocytes (FLS)	1140:1173	It also exhibited a higher inhibitory effect on the activity of IL-1β-converting enzyme and production of IL-1β in fibroblast-like synoviocytes (FLS).
25048214	4	12	theme	gene	289:292	arg1	ste					303:305	ste	303:305	ste	303:305	The Ebosin biosynthesis gene cluster (ste) consists of 27 ORFs and ste7 has previously been demonstrated to code for a fucosyltransferase, which plays an essential role in the formation of repeating sugar units during Ebosin production.
25048214	4	12	theme	gene	289:292	arg1	cluster					294:300	The Ebosin biosynthesis gene cluster	265:300	The Ebosin biosynthesis gene cluster (ste)	265:306	The Ebosin biosynthesis gene cluster (ste) consists of 27 ORFs and ste7 has previously been demonstrated to code for a fucosyltransferase, which plays an essential role in the formation of repeating sugar units during Ebosin production.
25048214	7	13	theme	interleukin-1	989:1001	arg1	activity					950:957	a higher binding activity	933:957	a higher binding activity for IL-1R	933:967	In an ELISA analysis, EPS-7 g exhibited a higher binding activity for IL-1R, as a competitor of interleukin-1, than that of Ebosin.
25048214	7	13	theme	interleukin-1	989:1001	arg1	competitor					975:984	a competitor	973:984	a competitor of interleukin-1	973:1001	In an ELISA analysis, EPS-7 g exhibited a higher binding activity for IL-1R, as a competitor of interleukin-1, than that of Ebosin.
25048214	6	14	theme	monosaccharide	748:761	arg1	composition					763:773	its monosaccharide composition	744:773	its monosaccharide composition	744:773	RESULTS This alteration resulted in a novel Ebosin derivative (EPS-7 g) with its monosaccharide composition dramatically changed, especially in the proportion of glucose which increased from 1.1% (Ebosin) to 84.01% (EPS-7 g).
25048214	4	15	theme	biosynthesis	276:287	arg1	ste					303:305	ste	303:305	ste	303:305	The Ebosin biosynthesis gene cluster (ste) consists of 27 ORFs and ste7 has previously been demonstrated to code for a fucosyltransferase, which plays an essential role in the formation of repeating sugar units during Ebosin production.
25048214	4	15	theme	biosynthesis	276:287	arg1	cluster					294:300	The Ebosin biosynthesis gene cluster	265:300	The Ebosin biosynthesis gene cluster (ste)	265:306	The Ebosin biosynthesis gene cluster (ste) consists of 27 ORFs and ste7 has previously been demonstrated to code for a fucosyltransferase, which plays an essential role in the formation of repeating sugar units during Ebosin production.
25048214	8	16	theme	IL-1β	1131:1135	arg1	activity					1077:1084	the activity	1073:1084	the activity of IL-1β-converting enzyme	1073:1111	It also exhibited a higher inhibitory effect on the activity of IL-1β-converting enzyme and production of IL-1β in fibroblast-like synoviocytes (FLS).
25048214	8	16	theme	IL-1β	1131:1135	arg1	production					1117:1126	production	1117:1126	production of IL-1β in fibroblast-like synoviocytes (FLS)	1117:1173	It also exhibited a higher inhibitory effect on the activity of IL-1β-converting enzyme and production of IL-1β in fibroblast-like synoviocytes (FLS).
25048214	8	17	theme	inhibitory	1052:1061	arg1	effect					1063:1068	a higher inhibitory effect	1043:1068	a higher inhibitory effect on the activity of IL-1β-converting enzyme and production of IL-1β in fibroblast-like synoviocytes (FLS)	1043:1173	It also exhibited a higher inhibitory effect on the activity of IL-1β-converting enzyme and production of IL-1β in fibroblast-like synoviocytes (FLS).
25048214	4	18	theme	repeating	454:462	arg1	units					470:474	repeating sugar units	454:474	repeating sugar units	454:474	The Ebosin biosynthesis gene cluster (ste) consists of 27 ORFs and ste7 has previously been demonstrated to code for a fucosyltransferase, which plays an essential role in the formation of repeating sugar units during Ebosin production.
25048214	10	19	theme	derivative	1365:1374	arg1	g					1382:1382	The new Ebosin derivative EPS-7 g	1350:1382	The new Ebosin derivative EPS-7 g	1350:1382	CONCLUSIONS The new Ebosin derivative EPS-7 g is more bioactive than Ebosin evaluated by a series of experiments.
25048214	10	19	theme	derivative	1365:1374	arg1	bioactive					1392:1400	bioactive	1392:1400	bioactive	1392:1400	CONCLUSIONS The new Ebosin derivative EPS-7 g is more bioactive than Ebosin evaluated by a series of experiments.
25048214	6	20	dep	RESULTS	667:673	arg1	resulted					691:698	resulted	691:698	resulted in a novel Ebosin derivative (EPS-7 g) with its monosaccharide composition	691:773	RESULTS This alteration resulted in a novel Ebosin derivative (EPS-7 g) with its monosaccharide composition dramatically changed, especially in the proportion of glucose which increased from 1.1% (Ebosin) to 84.01% (EPS-7 g).
25048214	9	21	theme	anti-inflammatory	1278:1294	arg1	activity					1296:1303	a significantly higher anti-inflammatory activity	1255:1303	a significantly higher anti-inflammatory activity of EPS-7 g	1255:1314	In addition, experiments with acute inflamed mice induced by croton oil showed a significantly higher anti-inflammatory activity of EPS-7 g compared with Ebosin.
25048214	10	22	theme	experiments	1439:1449	arg1	series					1429:1434	a series	1427:1434	a series of experiments	1427:1449	CONCLUSIONS The new Ebosin derivative EPS-7 g is more bioactive than Ebosin evaluated by a series of experiments.
25048214	9	23	theme	g	1314:1314	arg1	activity					1296:1303	a significantly higher anti-inflammatory activity	1255:1303	a significantly higher anti-inflammatory activity of EPS-7 g	1255:1314	In addition, experiments with acute inflamed mice induced by croton oil showed a significantly higher anti-inflammatory activity of EPS-7 g compared with Ebosin.
25048214	2	24	theme	novel	131:135	arg1	exopolysaccharide					137:153	a novel exopolysaccharide	129:153	a novel exopolysaccharide (EPS) produced by Streptomyces sp	129:187	BACKGROUND Ebosin is a novel exopolysaccharide (EPS) produced by Streptomyces sp.
25048214	2	24	theme	novel	131:135	arg1	Ebosin					119:124	BACKGROUND Ebosin	108:124	BACKGROUND Ebosin	108:124	BACKGROUND Ebosin is a novel exopolysaccharide (EPS) produced by Streptomyces sp.
25048214	2	24	theme	novel	131:135	arg1	EPS					156:158	EPS	156:158	EPS	156:158	BACKGROUND Ebosin is a novel exopolysaccharide (EPS) produced by Streptomyces sp.
25048214	3	25	theme	anti-rheumatic	222:235	arg1	activity					247:254	an anti-rheumatic arthritis activity	219:254	an anti-rheumatic arthritis activity	219:254	139 and evidenced to possess an anti-rheumatic arthritis activity in vivo.
25048214	5	26	theme	better	547:552	arg1	activity					554:561	better activity	547:561	better activity	547:561	Aiming to generate derivatives of Ebosin for better activity, we replaced ste7 with a gene encoding for a glucosyltransferase (gtf) from Streptococcus thermophilus.
25048214	8	27	theme	fibroblast-like	1140:1154	arg1	FLS					1170:1172	FLS	1170:1172	FLS	1170:1172	It also exhibited a higher inhibitory effect on the activity of IL-1β-converting enzyme and production of IL-1β in fibroblast-like synoviocytes (FLS).
25048214	8	27	theme	fibroblast-like	1140:1154	arg1	synoviocytes					1156:1167	fibroblast-like synoviocytes	1140:1167	fibroblast-like synoviocytes (FLS)	1140:1173	It also exhibited a higher inhibitory effect on the activity of IL-1β-converting enzyme and production of IL-1β in fibroblast-like synoviocytes (FLS).
25048214	3	28	theme	arthritis	237:245	arg1	activity					247:254	an anti-rheumatic arthritis activity	219:254	an anti-rheumatic arthritis activity	219:254	139 and evidenced to possess an anti-rheumatic arthritis activity in vivo.
25048214	10	29	theme	EPS-7	1376:1380	arg1	g					1382:1382	The new Ebosin derivative EPS-7 g	1350:1382	The new Ebosin derivative EPS-7 g	1350:1382	CONCLUSIONS The new Ebosin derivative EPS-7 g is more bioactive than Ebosin evaluated by a series of experiments.
25048214	10	29	theme	EPS-7	1376:1380	arg1	bioactive					1392:1400	bioactive	1392:1400	bioactive	1392:1400	CONCLUSIONS The new Ebosin derivative EPS-7 g is more bioactive than Ebosin evaluated by a series of experiments.
25048214	0	30	theme	Ebosin	23:28	arg1	derivative					30:39	an Ebosin derivative	20:39	an Ebosin derivative	20:39	Characterization of an Ebosin derivative produced by heterologous gene replacement in Streptomyces sp.
25048214	10	31	theme	new	1354:1356	arg1	g					1382:1382	The new Ebosin derivative EPS-7 g	1350:1382	The new Ebosin derivative EPS-7 g	1350:1382	CONCLUSIONS The new Ebosin derivative EPS-7 g is more bioactive than Ebosin evaluated by a series of experiments.
25048214	10	31	theme	new	1354:1356	arg1	bioactive					1392:1400	bioactive	1392:1400	bioactive	1392:1400	CONCLUSIONS The new Ebosin derivative EPS-7 g is more bioactive than Ebosin evaluated by a series of experiments.
25048214	8	32	from	IL-1β	1131:1135	arg1	FLS					1170:1172	FLS	1170:1172	FLS	1170:1172	It also exhibited a higher inhibitory effect on the activity of IL-1β-converting enzyme and production of IL-1β in fibroblast-like synoviocytes (FLS).
25048214	8	32	from	IL-1β	1131:1135	arg1	synoviocytes					1156:1167	fibroblast-like synoviocytes	1140:1167	fibroblast-like synoviocytes (FLS)	1140:1173	It also exhibited a higher inhibitory effect on the activity of IL-1β-converting enzyme and production of IL-1β in fibroblast-like synoviocytes (FLS).
25048214	2	33	theme	Streptomyces	173:184	arg1	sp					186:187	Streptomyces sp	173:187	Streptomyces sp	173:187	BACKGROUND Ebosin is a novel exopolysaccharide (EPS) produced by Streptomyces sp.
25048214	4	34	theme	Ebosin	269:274	arg1	ste					303:305	ste	303:305	ste	303:305	The Ebosin biosynthesis gene cluster (ste) consists of 27 ORFs and ste7 has previously been demonstrated to code for a fucosyltransferase, which plays an essential role in the formation of repeating sugar units during Ebosin production.
25048214	4	34	theme	Ebosin	269:274	arg1	cluster					294:300	The Ebosin biosynthesis gene cluster	265:300	The Ebosin biosynthesis gene cluster (ste)	265:306	The Ebosin biosynthesis gene cluster (ste) consists of 27 ORFs and ste7 has previously been demonstrated to code for a fucosyltransferase, which plays an essential role in the formation of repeating sugar units during Ebosin production.
25048214	8	35	theme	enzyme	1106:1111	arg1	activity					1077:1084	the activity	1073:1084	the activity of IL-1β-converting enzyme	1073:1111	It also exhibited a higher inhibitory effect on the activity of IL-1β-converting enzyme and production of IL-1β in fibroblast-like synoviocytes (FLS).
25048214	8	35	theme	enzyme	1106:1111	arg1	production					1117:1126	production	1117:1126	production of IL-1β in fibroblast-like synoviocytes (FLS)	1117:1173	It also exhibited a higher inhibitory effect on the activity of IL-1β-converting enzyme and production of IL-1β in fibroblast-like synoviocytes (FLS).
25048214	9	36	theme	croton	1237:1242	arg1	oil					1244:1246	croton oil	1237:1246	croton oil	1237:1246	In addition, experiments with acute inflamed mice induced by croton oil showed a significantly higher anti-inflammatory activity of EPS-7 g compared with Ebosin.
25048214	0	37	theme	derivative	30:39	arg1	Characterization					0:15	Characterization	0:15	Characterization of an Ebosin derivative	0:39	Characterization of an Ebosin derivative produced by heterologous gene replacement in Streptomyces sp.
25048214	8	38	theme	IL-1β-converting	1089:1104	arg1	enzyme					1106:1111	IL-1β-converting enzyme	1089:1111	IL-1β-converting enzyme	1089:1111	It also exhibited a higher inhibitory effect on the activity of IL-1β-converting enzyme and production of IL-1β in fibroblast-like synoviocytes (FLS).
25048214	11	39	theme	heterologous	1527:1538	arg1	replacement					1545:1555	heterologous gene replacement	1527:1555	heterologous gene replacement in Streptomyces	1527:1571	This is the first report demonstrating a modification of EPS structure via heterologous gene replacement in Streptomyces.
25048214	0	40	theme	heterologous	53:64	arg1	replacement					71:81	heterologous gene replacement	53:81	heterologous gene replacement	53:81	Characterization of an Ebosin derivative produced by heterologous gene replacement in Streptomyces sp.
25048214	9	41	theme	acute	1206:1210	arg1	mice					1221:1224	acute inflamed mice	1206:1224	acute inflamed mice induced by croton oil	1206:1246	In addition, experiments with acute inflamed mice induced by croton oil showed a significantly higher anti-inflammatory activity of EPS-7 g compared with Ebosin.
25048214	10	42	dep	CONCLUSIONS	1338:1348	arg1	g					1382:1382	The new Ebosin derivative EPS-7 g	1350:1382	The new Ebosin derivative EPS-7 g	1350:1382	CONCLUSIONS The new Ebosin derivative EPS-7 g is more bioactive than Ebosin evaluated by a series of experiments.
25048214	10	42	dep	CONCLUSIONS	1338:1348	arg1	bioactive					1392:1400	bioactive	1392:1400	bioactive	1392:1400	CONCLUSIONS The new Ebosin derivative EPS-7 g is more bioactive than Ebosin evaluated by a series of experiments.
25048214	7	43	theme	ELISA	899:903	arg1	analysis					905:912	an ELISA analysis	896:912	an ELISA analysis	896:912	In an ELISA analysis, EPS-7 g exhibited a higher binding activity for IL-1R, as a competitor of interleukin-1, than that of Ebosin.
25048214	11	44	theme	gene	1540:1543	arg1	replacement					1545:1555	heterologous gene replacement	1527:1555	heterologous gene replacement in Streptomyces	1527:1571	This is the first report demonstrating a modification of EPS structure via heterologous gene replacement in Streptomyces.
25048214	8	45	from	effect	1063:1068	arg1	activity					1077:1084	the activity	1073:1084	the activity of IL-1β-converting enzyme	1073:1111	It also exhibited a higher inhibitory effect on the activity of IL-1β-converting enzyme and production of IL-1β in fibroblast-like synoviocytes (FLS).
25048214	8	45	from	effect	1063:1068	arg1	production					1117:1126	production	1117:1126	production of IL-1β in fibroblast-like synoviocytes (FLS)	1117:1173	It also exhibited a higher inhibitory effect on the activity of IL-1β-converting enzyme and production of IL-1β in fibroblast-like synoviocytes (FLS).
25048214	7	46	theme	EPS-7	915:919	arg1	g					921:921	EPS-7 g	915:921	EPS-7 g	915:921	In an ELISA analysis, EPS-7 g exhibited a higher binding activity for IL-1R, as a competitor of interleukin-1, than that of Ebosin.
25048214	6	47	dep	glucose	829:835	arg1	the					811:813	the	811:813	the	811:813	RESULTS This alteration resulted in a novel Ebosin derivative (EPS-7 g) with its monosaccharide composition dramatically changed, especially in the proportion of glucose which increased from 1.1% (Ebosin) to 84.01% (EPS-7 g).
25048214	6	47	dep	glucose	829:835	arg1	proportion					815:824	proportion	815:824	proportion	815:824	RESULTS This alteration resulted in a novel Ebosin derivative (EPS-7 g) with its monosaccharide composition dramatically changed, especially in the proportion of glucose which increased from 1.1% (Ebosin) to 84.01% (EPS-7 g).
25048214	7	48	theme	higher	935:940	arg1	activity					950:957	a higher binding activity	933:957	a higher binding activity for IL-1R	933:967	In an ELISA analysis, EPS-7 g exhibited a higher binding activity for IL-1R, as a competitor of interleukin-1, than that of Ebosin.
25048214	7	48	theme	higher	935:940	arg1	competitor					975:984	a competitor	973:984	a competitor of interleukin-1	973:1001	In an ELISA analysis, EPS-7 g exhibited a higher binding activity for IL-1R, as a competitor of interleukin-1, than that of Ebosin.
25048214	5	49	theme	Ebosin	536:541	arg1	derivatives					521:531	derivatives	521:531	derivatives of Ebosin for better activity	521:561	Aiming to generate derivatives of Ebosin for better activity, we replaced ste7 with a gene encoding for a glucosyltransferase (gtf) from Streptococcus thermophilus.
25048214	11	50	theme	first	1464:1468	arg1	This					1452:1455	This	1452:1455	This	1452:1455	This is the first report demonstrating a modification of EPS structure via heterologous gene replacement in Streptomyces.
25048214	11	50	theme	first	1464:1468	arg1	report					1470:1475	the first report	1460:1475	the first report demonstrating a modification of EPS structure via heterologous gene replacement in Streptomyces	1460:1571	This is the first report demonstrating a modification of EPS structure via heterologous gene replacement in Streptomyces.
25048214	9	51	theme	higher	1271:1276	arg1	activity					1296:1303	a significantly higher anti-inflammatory activity	1255:1303	a significantly higher anti-inflammatory activity of EPS-7 g	1255:1314	In addition, experiments with acute inflamed mice induced by croton oil showed a significantly higher anti-inflammatory activity of EPS-7 g compared with Ebosin.
25048214	6	52	theme	Ebosin	711:716	arg1	derivative					718:727	a novel Ebosin derivative	703:727	a novel Ebosin derivative (EPS-7 g) with its monosaccharide composition	703:773	RESULTS This alteration resulted in a novel Ebosin derivative (EPS-7 g) with its monosaccharide composition dramatically changed, especially in the proportion of glucose which increased from 1.1% (Ebosin) to 84.01% (EPS-7 g).
25048214	6	52	theme	Ebosin	711:716	arg1	g					736:736	EPS-7 g	730:736	EPS-7 g	730:736	RESULTS This alteration resulted in a novel Ebosin derivative (EPS-7 g) with its monosaccharide composition dramatically changed, especially in the proportion of glucose which increased from 1.1% (Ebosin) to 84.01% (EPS-7 g).
25048214	8	53	from	synoviocytes	1156:1167	arg1	activity					1077:1084	the activity	1073:1084	the activity of IL-1β-converting enzyme	1073:1111	It also exhibited a higher inhibitory effect on the activity of IL-1β-converting enzyme and production of IL-1β in fibroblast-like synoviocytes (FLS).
25048214	8	53	from	synoviocytes	1156:1167	arg1	production					1117:1126	production	1117:1126	production of IL-1β in fibroblast-like synoviocytes (FLS)	1117:1173	It also exhibited a higher inhibitory effect on the activity of IL-1β-converting enzyme and production of IL-1β in fibroblast-like synoviocytes (FLS).
25048214	6	54	theme	novel	705:709	arg1	derivative					718:727	a novel Ebosin derivative	703:727	a novel Ebosin derivative (EPS-7 g) with its monosaccharide composition	703:773	RESULTS This alteration resulted in a novel Ebosin derivative (EPS-7 g) with its monosaccharide composition dramatically changed, especially in the proportion of glucose which increased from 1.1% (Ebosin) to 84.01% (EPS-7 g).
25048214	6	54	theme	novel	705:709	arg1	g					736:736	EPS-7 g	730:736	EPS-7 g	730:736	RESULTS This alteration resulted in a novel Ebosin derivative (EPS-7 g) with its monosaccharide composition dramatically changed, especially in the proportion of glucose which increased from 1.1% (Ebosin) to 84.01% (EPS-7 g).
25048214	9	55	with	experiments	1189:1199	arg1	mice					1221:1224	acute inflamed mice	1206:1224	acute inflamed mice induced by croton oil	1206:1246	In addition, experiments with acute inflamed mice induced by croton oil showed a significantly higher anti-inflammatory activity of EPS-7 g compared with Ebosin.
25048214	5	56	from	thermophilus	653:664	arg1	glucosyltransferase					608:626	a glucosyltransferase	606:626	a glucosyltransferase (gtf) from Streptococcus thermophilus	606:664	Aiming to generate derivatives of Ebosin for better activity, we replaced ste7 with a gene encoding for a glucosyltransferase (gtf) from Streptococcus thermophilus.
25048214	5	56	from	thermophilus	653:664	arg1	gtf					629:631	gtf	629:631	gtf	629:631	Aiming to generate derivatives of Ebosin for better activity, we replaced ste7 with a gene encoding for a glucosyltransferase (gtf) from Streptococcus thermophilus.
25048214	6	57	with	derivative	718:727	arg1	composition					763:773	its monosaccharide composition	744:773	its monosaccharide composition	744:773	RESULTS This alteration resulted in a novel Ebosin derivative (EPS-7 g) with its monosaccharide composition dramatically changed, especially in the proportion of glucose which increased from 1.1% (Ebosin) to 84.01% (EPS-7 g).
25048214	11	58	theme	structure	1513:1521	arg1	modification					1493:1504	a modification	1491:1504	a modification of EPS structure	1491:1521	This is the first report demonstrating a modification of EPS structure via heterologous gene replacement in Streptomyces.
25048214	0	59	theme	gene	66:69	arg1	replacement					71:81	heterologous gene replacement	53:81	heterologous gene replacement	53:81	Characterization of an Ebosin derivative produced by heterologous gene replacement in Streptomyces sp.
25048214	4	60	theme	units	470:474	arg1	formation					441:449	the formation	437:449	the formation of repeating sugar units during Ebosin production	437:499	The Ebosin biosynthesis gene cluster (ste) consists of 27 ORFs and ste7 has previously been demonstrated to code for a fucosyltransferase, which plays an essential role in the formation of repeating sugar units during Ebosin production.
25048214	9	61	theme	inflamed	1212:1219	arg1	mice					1221:1224	acute inflamed mice	1206:1224	acute inflamed mice induced by croton oil	1206:1246	In addition, experiments with acute inflamed mice induced by croton oil showed a significantly higher anti-inflammatory activity of EPS-7 g compared with Ebosin.
25048214	11	62	from	replacement	1545:1555	arg1	Streptomyces					1560:1571	Streptomyces	1560:1571	Streptomyces	1560:1571	This is the first report demonstrating a modification of EPS structure via heterologous gene replacement in Streptomyces.
24114597	3	0	theme	study	313:317	arg1	objective					295:303	The objective	291:303	The objective of this study	291:317	The objective of this study was to determine the influence of casein hydrolysates (CH) with molecular weight less than 3 kDa on cell viability, EPS synthesis and the enzyme activity involved in EPS synthesis during the co-culturing of Streptococcus thermophilus and Lactobacillus delbrueckii ssp.
24114597	9	1	theme	key	1453:1455	arg1	enzymes					1467:1473	key metabolic enzymes	1453:1473	key metabolic enzymes	1453:1473	CONCLUSION The influence of different CH (<3 kDa) on LAB viability, EPS production, EPS monomeric composition and activity levels of key metabolic enzymes was distinct.
24114597	9	2	dep	CONCLUSION	1320:1329	arg1	distinct					1479:1486	distinct	1479:1486	distinct	1479:1486	CONCLUSION The influence of different CH (<3 kDa) on LAB viability, EPS production, EPS monomeric composition and activity levels of key metabolic enzymes was distinct.
24114597	9	2	dep	CONCLUSION	1320:1329	arg1	influence					1335:1343	The influence	1331:1343	The influence of different CH (<3 kDa) on LAB viability, EPS production, EPS monomeric composition and activity levels of key metabolic enzymes	1331:1473	CONCLUSION The influence of different CH (<3 kDa) on LAB viability, EPS production, EPS monomeric composition and activity levels of key metabolic enzymes was distinct.
24114597	6	3	theme	time	906:909	arg1	extension					876:884	extension	876:884	extension of the fermentation time	876:909	The monosaccharide composition changed with extension of the fermentation time.
24114597	7	4	theme	EPS	1058:1060	arg1	synthesis					1062:1070	EPS synthesis	1058:1070	EPS synthesis	1058:1070	The activities of α-phosphoglucomutase, uridine 5'-diphosphate (UDP)-glucose pyrophosphorylase and UDP-galactose 4-epimerase were associated with EPS synthesis.
24114597	8	5	from	phase	1260:1264	arg1	activities					1087:1096	the activities	1083:1096	the activities of β-phosphoglucomutase and deoxythymadine 5'-diphosphate (dTDP)-glucose pyrophosphorylase involved in rhamnose synthesis	1083:1218	Moreover, the activities of β-phosphoglucomutase and deoxythymadine 5'-diphosphate (dTDP)-glucose pyrophosphorylase involved in rhamnose synthesis were very low at the exponential growth phase and could not be detected during other given periods.
24114597	8	5	from	phase	1260:1264	arg1	low					1230:1232	low	1230:1232	low	1230:1232	Moreover, the activities of β-phosphoglucomutase and deoxythymadine 5'-diphosphate (dTDP)-glucose pyrophosphorylase involved in rhamnose synthesis were very low at the exponential growth phase and could not be detected during other given periods.
24114597	9	6	theme	metabolic	1457:1465	arg1	enzymes					1467:1473	key metabolic enzymes	1453:1473	key metabolic enzymes	1453:1473	CONCLUSION The influence of different CH (<3 kDa) on LAB viability, EPS production, EPS monomeric composition and activity levels of key metabolic enzymes was distinct.
24114597	9	7	theme	monomeric	1408:1416	arg1	composition					1418:1428	EPS monomeric composition	1404:1428	EPS monomeric composition	1404:1428	CONCLUSION The influence of different CH (<3 kDa) on LAB viability, EPS production, EPS monomeric composition and activity levels of key metabolic enzymes was distinct.
24114597	6	8	theme	fermentation	893:904	arg1	time					906:909	the fermentation time	889:909	the fermentation time	889:909	The monosaccharide composition changed with extension of the fermentation time.
24114597	7	9	dep	α-phosphoglucomutase	930:949	arg1	-glucose					980:987	-glucose	980:987	-glucose	980:987	The activities of α-phosphoglucomutase, uridine 5'-diphosphate (UDP)-glucose pyrophosphorylase and UDP-galactose 4-epimerase were associated with EPS synthesis.
24114597	0	10	from	Influence	0:8	arg1	synthesis					54:62	exopolysaccharide synthesis	36:62	exopolysaccharide synthesis by Streptococcus thermophilus and Lactobacillus delbrueckii ssp	36:126	Influence of casein hydrolysates on exopolysaccharide synthesis by Streptococcus thermophilus and Lactobacillus delbrueckii ssp.
24114597	9	11	theme	enzymes	1467:1473	arg1	viability					1377:1385	LAB viability	1373:1385	LAB viability	1373:1385	CONCLUSION The influence of different CH (<3 kDa) on LAB viability, EPS production, EPS monomeric composition and activity levels of key metabolic enzymes was distinct.
24114597	9	11	theme	enzymes	1467:1473	arg1	production					1392:1401	EPS production	1388:1401	EPS production	1388:1401	CONCLUSION The influence of different CH (<3 kDa) on LAB viability, EPS production, EPS monomeric composition and activity levels of key metabolic enzymes was distinct.
24114597	9	11	theme	enzymes	1467:1473	arg1	composition					1418:1428	EPS monomeric composition	1404:1428	EPS monomeric composition	1404:1428	CONCLUSION The influence of different CH (<3 kDa) on LAB viability, EPS production, EPS monomeric composition and activity levels of key metabolic enzymes was distinct.
24114597	9	11	theme	enzymes	1467:1473	arg1	levels					1443:1448	activity levels	1434:1448	activity levels	1434:1448	CONCLUSION The influence of different CH (<3 kDa) on LAB viability, EPS production, EPS monomeric composition and activity levels of key metabolic enzymes was distinct.
24114597	3	12	theme	casein	353:358	arg1	CH					374:375	CH	374:375	CH	374:375	The objective of this study was to determine the influence of casein hydrolysates (CH) with molecular weight less than 3 kDa on cell viability, EPS synthesis and the enzyme activity involved in EPS synthesis during the co-culturing of Streptococcus thermophilus and Lactobacillus delbrueckii ssp.
24114597	3	12	theme	casein	353:358	arg1	hydrolysates					360:371	casein hydrolysates	353:371	casein hydrolysates (CH) with molecular weight less than 3 kDa	353:414	The objective of this study was to determine the influence of casein hydrolysates (CH) with molecular weight less than 3 kDa on cell viability, EPS synthesis and the enzyme activity involved in EPS synthesis during the co-culturing of Streptococcus thermophilus and Lactobacillus delbrueckii ssp.
24114597	9	13	theme	activity	1434:1441	arg1	levels					1443:1448	activity levels	1434:1448	activity levels	1434:1448	CONCLUSION The influence of different CH (<3 kDa) on LAB viability, EPS production, EPS monomeric composition and activity levels of key metabolic enzymes was distinct.
24114597	3	14	with	hydrolysates	360:371	arg1	weight					393:398	molecular weight	383:398	molecular weight less than 3 kDa	383:414	The objective of this study was to determine the influence of casein hydrolysates (CH) with molecular weight less than 3 kDa on cell viability, EPS synthesis and the enzyme activity involved in EPS synthesis during the co-culturing of Streptococcus thermophilus and Lactobacillus delbrueckii ssp.
24114597	8	15	theme	β-phosphoglucomutase	1101:1120	arg1	pyrophosphorylase					1171:1187	β-phosphoglucomutase and deoxythymadine 5'-diphosphate (dTDP)-glucose pyrophosphorylase	1101:1187	β-phosphoglucomutase and deoxythymadine 5'-diphosphate (dTDP)-glucose pyrophosphorylase involved in rhamnose synthesis	1101:1218	Moreover, the activities of β-phosphoglucomutase and deoxythymadine 5'-diphosphate (dTDP)-glucose pyrophosphorylase involved in rhamnose synthesis were very low at the exponential growth phase and could not be detected during other given periods.
24114597	2	16	theme	research	173:180	arg1	lot					154:156	A lot	152:156	A lot of interesting research	152:180	BACKGROUND A lot of interesting research has been undertaken to enhance the yield of exopolysaccharides (EPS) produced by lactic acid bacteria (LAB).
24114597	2	16	theme	research	173:180	arg1	research					173:180	interesting research	161:180	interesting research	161:180	BACKGROUND A lot of interesting research has been undertaken to enhance the yield of exopolysaccharides (EPS) produced by lactic acid bacteria (LAB).
24114597	7	17	theme	UDP-galactose	1011:1023	arg1	4-epimerase					1025:1035	UDP-galactose 4-epimerase	1011:1035	UDP-galactose 4-epimerase	1011:1035	The activities of α-phosphoglucomutase, uridine 5'-diphosphate (UDP)-glucose pyrophosphorylase and UDP-galactose 4-epimerase were associated with EPS synthesis.
24114597	8	18	theme	given	1305:1309	arg1	periods					1311:1317	other given periods	1299:1317	other given periods	1299:1317	Moreover, the activities of β-phosphoglucomutase and deoxythymadine 5'-diphosphate (dTDP)-glucose pyrophosphorylase involved in rhamnose synthesis were very low at the exponential growth phase and could not be detected during other given periods.
24114597	8	19	theme	-glucose	1162:1169	arg1	pyrophosphorylase					1171:1187	β-phosphoglucomutase and deoxythymadine 5'-diphosphate (dTDP)-glucose pyrophosphorylase	1101:1187	β-phosphoglucomutase and deoxythymadine 5'-diphosphate (dTDP)-glucose pyrophosphorylase involved in rhamnose synthesis	1101:1218	Moreover, the activities of β-phosphoglucomutase and deoxythymadine 5'-diphosphate (dTDP)-glucose pyrophosphorylase involved in rhamnose synthesis were very low at the exponential growth phase and could not be detected during other given periods.
24114597	2	20	theme	interesting	161:171	arg1	research					173:180	interesting research	161:180	interesting research	161:180	BACKGROUND A lot of interesting research has been undertaken to enhance the yield of exopolysaccharides (EPS) produced by lactic acid bacteria (LAB).
24114597	7	21	theme	4-epimerase	1025:1035	arg1	activities					916:925	The activities	912:925	The activities of α-phosphoglucomutase, uridine 5'-diphosphate (UDP)-glucose pyrophosphorylase and UDP-galactose 4-epimerase	912:1035	The activities of α-phosphoglucomutase, uridine 5'-diphosphate (UDP)-glucose pyrophosphorylase and UDP-galactose 4-epimerase were associated with EPS synthesis.
24114597	5	22	theme	1.0:2.4:1.5	819:829	arg1	ratio					810:814	a molar ratio	802:814	a molar ratio of 1.0:2.4:1.5	802:829	At 24 h, EPS were composed of galactose, glucose and rhamnose in a molar ratio of 1.0:2.4:1.5.
24114597	4	23	from	°C.	633:635	arg1	MRS					602:604	bulgaricus in MRS broth	588:610	bulgaricus in MRS broth for 72 h at 37 ± 0.1 °C. RESULTS The highest EPS yield (150.1 mg L⁻¹)	588:680	bulgaricus in MRS broth for 72 h at 37 ± 0.1 °C. RESULTS The highest EPS yield (150.1 mg L⁻¹) was obtained on CH prepared with papain (CHP) at 48 h.
24114597	9	24	theme	LAB	1373:1375	arg1	viability					1377:1385	LAB viability	1373:1385	LAB viability	1373:1385	CONCLUSION The influence of different CH (<3 kDa) on LAB viability, EPS production, EPS monomeric composition and activity levels of key metabolic enzymes was distinct.
24114597	8	25	theme	deoxythymadine	1126:1139	arg1	5'-diphosphate					1141:1154	deoxythymadine 5'-diphosphate	1126:1154	deoxythymadine 5'-diphosphate	1126:1154	Moreover, the activities of β-phosphoglucomutase and deoxythymadine 5'-diphosphate (dTDP)-glucose pyrophosphorylase involved in rhamnose synthesis were very low at the exponential growth phase and could not be detected during other given periods.
24114597	3	26	dep	weight	393:398	arg1	kDa					412:414	less than 3 kDa	400:414	molecular weight less than 3 kDa	383:414	The objective of this study was to determine the influence of casein hydrolysates (CH) with molecular weight less than 3 kDa on cell viability, EPS synthesis and the enzyme activity involved in EPS synthesis during the co-culturing of Streptococcus thermophilus and Lactobacillus delbrueckii ssp.
24114597	3	27	theme	thermophilus	540:551	arg1	co-culturing					510:521	the co-culturing	506:521	the co-culturing of Streptococcus thermophilus and Lactobacillus delbrueckii ssp	506:585	The objective of this study was to determine the influence of casein hydrolysates (CH) with molecular weight less than 3 kDa on cell viability, EPS synthesis and the enzyme activity involved in EPS synthesis during the co-culturing of Streptococcus thermophilus and Lactobacillus delbrueckii ssp.
24114597	8	28	theme	rhamnose	1201:1208	arg1	synthesis					1210:1218	rhamnose synthesis	1201:1218	rhamnose synthesis	1201:1218	Moreover, the activities of β-phosphoglucomutase and deoxythymadine 5'-diphosphate (dTDP)-glucose pyrophosphorylase involved in rhamnose synthesis were very low at the exponential growth phase and could not be detected during other given periods.
24114597	3	29	theme	hydrolysates	360:371	arg1	influence					340:348	the influence	336:348	the influence of casein hydrolysates (CH) with molecular weight less than 3 kDa on cell viability, EPS synthesis and the enzyme activity involved in EPS synthesis	336:497	The objective of this study was to determine the influence of casein hydrolysates (CH) with molecular weight less than 3 kDa on cell viability, EPS synthesis and the enzyme activity involved in EPS synthesis during the co-culturing of Streptococcus thermophilus and Lactobacillus delbrueckii ssp.
24114597	4	30	dep	°C.	633:635	arg1	RESULTS					637:643	RESULTS	637:643	RESULTS The highest EPS yield (150.1 mg L⁻¹)	637:680	bulgaricus in MRS broth for 72 h at 37 ± 0.1 °C. RESULTS The highest EPS yield (150.1 mg L⁻¹) was obtained on CH prepared with papain (CHP) at 48 h.
24114597	4	31	dep	mg	674:675	arg1	L⁻¹					677:679	L⁻¹	677:679	L⁻¹	677:679	bulgaricus in MRS broth for 72 h at 37 ± 0.1 °C. RESULTS The highest EPS yield (150.1 mg L⁻¹) was obtained on CH prepared with papain (CHP) at 48 h.
24114597	9	32	theme	EPS	1388:1390	arg1	production					1392:1401	EPS production	1388:1401	EPS production	1388:1401	CONCLUSION The influence of different CH (<3 kDa) on LAB viability, EPS production, EPS monomeric composition and activity levels of key metabolic enzymes was distinct.
24114597	0	33	theme	casein	13:18	arg1	hydrolysates					20:31	casein hydrolysates	13:31	casein hydrolysates	13:31	Influence of casein hydrolysates on exopolysaccharide synthesis by Streptococcus thermophilus and Lactobacillus delbrueckii ssp.
24114597	3	34	theme	EPS	435:437	arg1	synthesis					439:447	EPS synthesis	435:447	EPS synthesis	435:447	The objective of this study was to determine the influence of casein hydrolysates (CH) with molecular weight less than 3 kDa on cell viability, EPS synthesis and the enzyme activity involved in EPS synthesis during the co-culturing of Streptococcus thermophilus and Lactobacillus delbrueckii ssp.
24114597	0	35	theme	exopolysaccharide	36:52	arg1	synthesis					54:62	exopolysaccharide synthesis	36:62	exopolysaccharide synthesis by Streptococcus thermophilus and Lactobacillus delbrueckii ssp	36:126	Influence of casein hydrolysates on exopolysaccharide synthesis by Streptococcus thermophilus and Lactobacillus delbrueckii ssp.
24114597	5	36	theme	molar	804:808	arg1	ratio					810:814	a molar ratio	802:814	a molar ratio of 1.0:2.4:1.5	802:829	At 24 h, EPS were composed of galactose, glucose and rhamnose in a molar ratio of 1.0:2.4:1.5.
24114597	3	37	from	influence	340:348	arg1	viability					424:432	cell viability	419:432	cell viability	419:432	The objective of this study was to determine the influence of casein hydrolysates (CH) with molecular weight less than 3 kDa on cell viability, EPS synthesis and the enzyme activity involved in EPS synthesis during the co-culturing of Streptococcus thermophilus and Lactobacillus delbrueckii ssp.
24114597	3	37	from	influence	340:348	arg1	synthesis					439:447	EPS synthesis	435:447	EPS synthesis	435:447	The objective of this study was to determine the influence of casein hydrolysates (CH) with molecular weight less than 3 kDa on cell viability, EPS synthesis and the enzyme activity involved in EPS synthesis during the co-culturing of Streptococcus thermophilus and Lactobacillus delbrueckii ssp.
24114597	3	37	from	influence	340:348	arg1	activity					464:471	the enzyme activity	453:471	the enzyme activity	453:471	The objective of this study was to determine the influence of casein hydrolysates (CH) with molecular weight less than 3 kDa on cell viability, EPS synthesis and the enzyme activity involved in EPS synthesis during the co-culturing of Streptococcus thermophilus and Lactobacillus delbrueckii ssp.
24114597	3	38	theme	ssp	583:585	arg1	co-culturing					510:521	the co-culturing	506:521	the co-culturing of Streptococcus thermophilus and Lactobacillus delbrueckii ssp	506:585	The objective of this study was to determine the influence of casein hydrolysates (CH) with molecular weight less than 3 kDa on cell viability, EPS synthesis and the enzyme activity involved in EPS synthesis during the co-culturing of Streptococcus thermophilus and Lactobacillus delbrueckii ssp.
24114597	3	39	theme	cell	419:422	arg1	viability					424:432	cell viability	419:432	cell viability	419:432	The objective of this study was to determine the influence of casein hydrolysates (CH) with molecular weight less than 3 kDa on cell viability, EPS synthesis and the enzyme activity involved in EPS synthesis during the co-culturing of Streptococcus thermophilus and Lactobacillus delbrueckii ssp.
24114597	10	40	theme	amino	1549:1553	arg1	acids					1555:1559	amino acids	1549:1559	amino acids	1549:1559	Besides, their influence was related to the distribution of amino acids.
24114597	2	41	dep	BACKGROUND	141:150	arg1	undertaken					191:200	undertaken	191:200	has been undertaken to enhance the yield of exopolysaccharides (EPS) produced by lactic acid bacteria (LAB)	182:288	BACKGROUND A lot of interesting research has been undertaken to enhance the yield of exopolysaccharides (EPS) produced by lactic acid bacteria (LAB).
24114597	0	42	theme	hydrolysates	20:31	arg1	Influence					0:8	Influence	0:8	Influence of casein hydrolysates on exopolysaccharide synthesis by Streptococcus thermophilus and Lactobacillus delbrueckii ssp.	0:127	Influence of casein hydrolysates on exopolysaccharide synthesis by Streptococcus thermophilus and Lactobacillus delbrueckii ssp.
24114597	8	43	theme	growth	1253:1258	arg1	phase					1260:1264	the exponential growth phase	1237:1264	the exponential growth phase	1237:1264	Moreover, the activities of β-phosphoglucomutase and deoxythymadine 5'-diphosphate (dTDP)-glucose pyrophosphorylase involved in rhamnose synthesis were very low at the exponential growth phase and could not be detected during other given periods.
24114597	8	44	theme	pyrophosphorylase	1171:1187	arg1	activities					1087:1096	the activities	1083:1096	the activities of β-phosphoglucomutase and deoxythymadine 5'-diphosphate (dTDP)-glucose pyrophosphorylase involved in rhamnose synthesis	1083:1218	Moreover, the activities of β-phosphoglucomutase and deoxythymadine 5'-diphosphate (dTDP)-glucose pyrophosphorylase involved in rhamnose synthesis were very low at the exponential growth phase and could not be detected during other given periods.
24114597	8	44	theme	pyrophosphorylase	1171:1187	arg1	low					1230:1232	low	1230:1232	low	1230:1232	Moreover, the activities of β-phosphoglucomutase and deoxythymadine 5'-diphosphate (dTDP)-glucose pyrophosphorylase involved in rhamnose synthesis were very low at the exponential growth phase and could not be detected during other given periods.
24114597	6	45	theme	monosaccharide	836:849	arg1	composition					851:861	The monosaccharide composition	832:861	The monosaccharide composition	832:861	The monosaccharide composition changed with extension of the fermentation time.
24114597	7	46	theme	pyrophosphorylase	989:1005	arg1	activities					916:925	The activities	912:925	The activities of α-phosphoglucomutase, uridine 5'-diphosphate (UDP)-glucose pyrophosphorylase and UDP-galactose 4-epimerase	912:1035	The activities of α-phosphoglucomutase, uridine 5'-diphosphate (UDP)-glucose pyrophosphorylase and UDP-galactose 4-epimerase were associated with EPS synthesis.
24114597	3	47	theme	Lactobacillus	557:569	arg1	ssp					583:585	Lactobacillus delbrueckii ssp	557:585	Lactobacillus delbrueckii ssp	557:585	The objective of this study was to determine the influence of casein hydrolysates (CH) with molecular weight less than 3 kDa on cell viability, EPS synthesis and the enzyme activity involved in EPS synthesis during the co-culturing of Streptococcus thermophilus and Lactobacillus delbrueckii ssp.
24114597	9	48	from	influence	1335:1343	arg1	viability					1377:1385	LAB viability	1373:1385	LAB viability	1373:1385	CONCLUSION The influence of different CH (<3 kDa) on LAB viability, EPS production, EPS monomeric composition and activity levels of key metabolic enzymes was distinct.
24114597	9	48	from	influence	1335:1343	arg1	production					1392:1401	EPS production	1388:1401	EPS production	1388:1401	CONCLUSION The influence of different CH (<3 kDa) on LAB viability, EPS production, EPS monomeric composition and activity levels of key metabolic enzymes was distinct.
24114597	9	48	from	influence	1335:1343	arg1	composition					1418:1428	EPS monomeric composition	1404:1428	EPS monomeric composition	1404:1428	CONCLUSION The influence of different CH (<3 kDa) on LAB viability, EPS production, EPS monomeric composition and activity levels of key metabolic enzymes was distinct.
24114597	9	48	from	influence	1335:1343	arg1	levels					1443:1448	activity levels	1434:1448	activity levels	1434:1448	CONCLUSION The influence of different CH (<3 kDa) on LAB viability, EPS production, EPS monomeric composition and activity levels of key metabolic enzymes was distinct.
24114597	8	49	theme	exponential	1241:1251	arg1	phase					1260:1264	the exponential growth phase	1237:1264	the exponential growth phase	1237:1264	Moreover, the activities of β-phosphoglucomutase and deoxythymadine 5'-diphosphate (dTDP)-glucose pyrophosphorylase involved in rhamnose synthesis were very low at the exponential growth phase and could not be detected during other given periods.
24114597	3	50	theme	molecular	383:391	arg1	weight					393:398	molecular weight	383:398	molecular weight less than 3 kDa	383:414	The objective of this study was to determine the influence of casein hydrolysates (CH) with molecular weight less than 3 kDa on cell viability, EPS synthesis and the enzyme activity involved in EPS synthesis during the co-culturing of Streptococcus thermophilus and Lactobacillus delbrueckii ssp.
24114597	2	51	theme	exopolysaccharides	226:243	arg1	yield					217:221	the yield	213:221	the yield of exopolysaccharides (EPS) produced by lactic acid bacteria (LAB)	213:288	BACKGROUND A lot of interesting research has been undertaken to enhance the yield of exopolysaccharides (EPS) produced by lactic acid bacteria (LAB).
24114597	3	52	theme	delbrueckii	571:581	arg1	ssp					583:585	Lactobacillus delbrueckii ssp	557:585	Lactobacillus delbrueckii ssp	557:585	The objective of this study was to determine the influence of casein hydrolysates (CH) with molecular weight less than 3 kDa on cell viability, EPS synthesis and the enzyme activity involved in EPS synthesis during the co-culturing of Streptococcus thermophilus and Lactobacillus delbrueckii ssp.
24114597	3	53	theme	enzyme	457:462	arg1	activity					464:471	the enzyme activity	453:471	the enzyme activity	453:471	The objective of this study was to determine the influence of casein hydrolysates (CH) with molecular weight less than 3 kDa on cell viability, EPS synthesis and the enzyme activity involved in EPS synthesis during the co-culturing of Streptococcus thermophilus and Lactobacillus delbrueckii ssp.
24114597	10	54	theme	acids	1555:1559	arg1	distribution					1533:1544	the distribution	1529:1544	the distribution of amino acids	1529:1559	Besides, their influence was related to the distribution of amino acids.
24114597	7	55	theme	α-phosphoglucomutase	930:949	arg1	pyrophosphorylase					989:1005	α-phosphoglucomutase, uridine 5'-diphosphate (UDP)-glucose pyrophosphorylase	930:1005	α-phosphoglucomutase, uridine 5'-diphosphate (UDP)-glucose pyrophosphorylase	930:1005	The activities of α-phosphoglucomutase, uridine 5'-diphosphate (UDP)-glucose pyrophosphorylase and UDP-galactose 4-epimerase were associated with EPS synthesis.
24114597	4	56	theme	in	599:600	arg1	MRS					602:604	bulgaricus in MRS broth	588:610	bulgaricus in MRS broth for 72 h at 37 ± 0.1 °C. RESULTS The highest EPS yield (150.1 mg L⁻¹)	588:680	bulgaricus in MRS broth for 72 h at 37 ± 0.1 °C. RESULTS The highest EPS yield (150.1 mg L⁻¹) was obtained on CH prepared with papain (CHP) at 48 h.
24114597	8	57	theme	other	1299:1303	arg1	periods					1311:1317	other given periods	1299:1317	other given periods	1299:1317	Moreover, the activities of β-phosphoglucomutase and deoxythymadine 5'-diphosphate (dTDP)-glucose pyrophosphorylase involved in rhamnose synthesis were very low at the exponential growth phase and could not be detected during other given periods.
24114597	2	58	theme	acid	270:273	arg1	LAB					285:287	LAB	285:287	LAB	285:287	BACKGROUND A lot of interesting research has been undertaken to enhance the yield of exopolysaccharides (EPS) produced by lactic acid bacteria (LAB).
24114597	2	58	theme	acid	270:273	arg1	bacteria					275:282	lactic acid bacteria	263:282	lactic acid bacteria (LAB)	263:288	BACKGROUND A lot of interesting research has been undertaken to enhance the yield of exopolysaccharides (EPS) produced by lactic acid bacteria (LAB).
24114597	4	59	theme	highest	649:655	arg1	yield					661:665	The highest EPS yield	645:665	The highest EPS yield (150.1 mg L⁻¹)	645:680	bulgaricus in MRS broth for 72 h at 37 ± 0.1 °C. RESULTS The highest EPS yield (150.1 mg L⁻¹) was obtained on CH prepared with papain (CHP) at 48 h.
24114597	4	59	theme	highest	649:655	arg1	mg					674:675	150.1 mg L⁻¹	668:679	150.1 mg L⁻¹	668:679	bulgaricus in MRS broth for 72 h at 37 ± 0.1 °C. RESULTS The highest EPS yield (150.1 mg L⁻¹) was obtained on CH prepared with papain (CHP) at 48 h.
24114597	4	60	theme	bulgaricus	588:597	arg1	MRS					602:604	bulgaricus in MRS broth	588:610	bulgaricus in MRS broth for 72 h at 37 ± 0.1 °C. RESULTS The highest EPS yield (150.1 mg L⁻¹)	588:680	bulgaricus in MRS broth for 72 h at 37 ± 0.1 °C. RESULTS The highest EPS yield (150.1 mg L⁻¹) was obtained on CH prepared with papain (CHP) at 48 h.
24114597	0	61	theme	thermophilus	81:92	arg1	ssp					124:126	Streptococcus thermophilus and Lactobacillus delbrueckii ssp	67:126	Streptococcus thermophilus and Lactobacillus delbrueckii ssp	67:126	Influence of casein hydrolysates on exopolysaccharide synthesis by Streptococcus thermophilus and Lactobacillus delbrueckii ssp.
24114597	9	62	theme	EPS	1404:1406	arg1	composition					1418:1428	EPS monomeric composition	1404:1428	EPS monomeric composition	1404:1428	CONCLUSION The influence of different CH (<3 kDa) on LAB viability, EPS production, EPS monomeric composition and activity levels of key metabolic enzymes was distinct.
24114597	2	63	theme	lactic	263:268	arg1	LAB					285:287	LAB	285:287	LAB	285:287	BACKGROUND A lot of interesting research has been undertaken to enhance the yield of exopolysaccharides (EPS) produced by lactic acid bacteria (LAB).
24114597	2	63	theme	lactic	263:268	arg1	bacteria					275:282	lactic acid bacteria	263:282	lactic acid bacteria (LAB)	263:288	BACKGROUND A lot of interesting research has been undertaken to enhance the yield of exopolysaccharides (EPS) produced by lactic acid bacteria (LAB).
24114597	4	64	theme	EPS	657:659	arg1	yield					661:665	The highest EPS yield	645:665	The highest EPS yield (150.1 mg L⁻¹)	645:680	bulgaricus in MRS broth for 72 h at 37 ± 0.1 °C. RESULTS The highest EPS yield (150.1 mg L⁻¹) was obtained on CH prepared with papain (CHP) at 48 h.
24114597	4	64	theme	EPS	657:659	arg1	mg					674:675	150.1 mg L⁻¹	668:679	150.1 mg L⁻¹	668:679	bulgaricus in MRS broth for 72 h at 37 ± 0.1 °C. RESULTS The highest EPS yield (150.1 mg L⁻¹) was obtained on CH prepared with papain (CHP) at 48 h.
24114597	9	65	theme	different	1348:1356	arg1	CH					1358:1359	different CH	1348:1359	different CH (<3 kDa)	1348:1368	CONCLUSION The influence of different CH (<3 kDa) on LAB viability, EPS production, EPS monomeric composition and activity levels of key metabolic enzymes was distinct.
24114597	9	65	theme	different	1348:1356	arg1	kDa					1365:1367	<3 kDa	1362:1367	<3 kDa	1362:1367	CONCLUSION The influence of different CH (<3 kDa) on LAB viability, EPS production, EPS monomeric composition and activity levels of key metabolic enzymes was distinct.
24114597	8	66	theme	5'-diphosphate	1141:1154	arg1	pyrophosphorylase					1171:1187	β-phosphoglucomutase and deoxythymadine 5'-diphosphate (dTDP)-glucose pyrophosphorylase	1101:1187	β-phosphoglucomutase and deoxythymadine 5'-diphosphate (dTDP)-glucose pyrophosphorylase involved in rhamnose synthesis	1101:1218	Moreover, the activities of β-phosphoglucomutase and deoxythymadine 5'-diphosphate (dTDP)-glucose pyrophosphorylase involved in rhamnose synthesis were very low at the exponential growth phase and could not be detected during other given periods.
24114597	3	67	theme	EPS	485:487	arg1	synthesis					489:497	EPS synthesis	485:497	EPS synthesis	485:497	The objective of this study was to determine the influence of casein hydrolysates (CH) with molecular weight less than 3 kDa on cell viability, EPS synthesis and the enzyme activity involved in EPS synthesis during the co-culturing of Streptococcus thermophilus and Lactobacillus delbrueckii ssp.
24114597	9	68	theme	CH	1358:1359	arg1	distinct					1479:1486	distinct	1479:1486	distinct	1479:1486	CONCLUSION The influence of different CH (<3 kDa) on LAB viability, EPS production, EPS monomeric composition and activity levels of key metabolic enzymes was distinct.
24114597	9	68	theme	CH	1358:1359	arg1	influence					1335:1343	The influence	1331:1343	The influence of different CH (<3 kDa) on LAB viability, EPS production, EPS monomeric composition and activity levels of key metabolic enzymes	1331:1473	CONCLUSION The influence of different CH (<3 kDa) on LAB viability, EPS production, EPS monomeric composition and activity levels of key metabolic enzymes was distinct.
24114597	0	69	theme	delbrueckii	112:122	arg1	ssp					124:126	Streptococcus thermophilus and Lactobacillus delbrueckii ssp	67:126	Streptococcus thermophilus and Lactobacillus delbrueckii ssp	67:126	Influence of casein hydrolysates on exopolysaccharide synthesis by Streptococcus thermophilus and Lactobacillus delbrueckii ssp.
24559734	1	0	theme	polysaccharide	324:337	arg1	cells					274:278	cells	274:278	cells	274:278	Acetobacter species are well known to have the ability to grow floating on the surface of the medium by producing pellicle, which consists of cells and a self-produced matrix of cell-attached polysaccharide.
24559734	1	0	theme	polysaccharide	324:337	arg1	matrix					300:305	a self-produced matrix	284:305	a self-produced matrix of cell-attached polysaccharide	284:337	Acetobacter species are well known to have the ability to grow floating on the surface of the medium by producing pellicle, which consists of cells and a self-produced matrix of cell-attached polysaccharide.
24559734	5	1	from	glucose	867:873	arg1	1:8					888:890	the ratio 1:8	878:890	the ratio 1:8	878:890	For example, the pellicle polysaccharide of SL13E-2 is composed of rhamnose and glucose in the ratio 1:8, and that of SL13E-4 and mesophilic A. pasteurianus NBRC3191 consists of rhamnose, glucose and xylose in the ratio 1:5:2 and 1:4:2, respectively.
24559734	4	2	theme	pellicle	705:712	arg1	polysaccharides					714:728	the pellicle polysaccharides	701:728	the pellicle polysaccharides of these A. pasteurianus strains	701:761	The monosaccharide composition of the pellicle polysaccharides of these A. pasteurianus strains was found to be varied.
24559734	6	3	from	galactose	1155:1163	arg1	2:2:1					1178:1182	the ratio 2:2:1	1168:1182	the ratio 2:2:1	1168:1182	On the other hand, the pellicle polysaccharides of SL13E-3 and SKU1108 strains are composed of rhamnose, glucose and galactose in the ratio 2:2:1 and 1:5:2.5, respectively.
24559734	0	4	theme	polysaccharides	85:99	arg1	characterization					61:76	characterization	61:76	characterization of the polysaccharides and of the induction patterns	61:129	Pellicle of thermotolerant Acetobacter pasteurianus strains: characterization of the polysaccharides and of the induction patterns.
24559734	8	5	theme	molecules	1557:1565	arg1	number					1547:1552	the number	1543:1552	the number of molecules of the polysaccharides	1543:1588	The size and sugar composition of the polysaccharides obtained from cells induced by ethanol and by uninduced cells were the same, indicating that the number of molecules of the polysaccharides had increased but the polysaccharide molecule remained unchanged.
24559734	0	6	dep	strains	52:58	arg1	characterization					61:76	characterization	61:76	characterization of the polysaccharides and of the induction patterns	61:129	Pellicle of thermotolerant Acetobacter pasteurianus strains: characterization of the polysaccharides and of the induction patterns.
24559734	6	7	theme	strains	1109:1115	arg1	polysaccharides					1070:1084	the pellicle polysaccharides	1057:1084	the pellicle polysaccharides of SL13E-3 and SKU1108 strains	1057:1115	On the other hand, the pellicle polysaccharides of SL13E-3 and SKU1108 strains are composed of rhamnose, glucose and galactose in the ratio 2:2:1 and 1:5:2.5, respectively.
24559734	8	8	theme	polysaccharides	1574:1588	arg1	molecules					1557:1565	molecules	1557:1565	molecules of the polysaccharides	1557:1588	The size and sugar composition of the polysaccharides obtained from cells induced by ethanol and by uninduced cells were the same, indicating that the number of molecules of the polysaccharides had increased but the polysaccharide molecule remained unchanged.
24559734	5	9	theme	mesophilic	917:926	arg1	pasteurianus					931:942	SL13E-4 and mesophilic A. pasteurianus NBRC3191	905:951	SL13E-4 and mesophilic A. pasteurianus NBRC3191	905:951	For example, the pellicle polysaccharide of SL13E-2 is composed of rhamnose and glucose in the ratio 1:8, and that of SL13E-4 and mesophilic A. pasteurianus NBRC3191 consists of rhamnose, glucose and xylose in the ratio 1:5:2 and 1:4:2, respectively.
24559734	1	10	contain	have	170:173	arg1	species					144:150	Acetobacter species	132:150	Acetobacter species	132:150	Acetobacter species are well known to have the ability to grow floating on the surface of the medium by producing pellicle, which consists of cells and a self-produced matrix of cell-attached polysaccharide.
24559734	1	10	contain	have	170:173	arg2	ability					179:185	the ability to grow floating on the surface of the medium by producing pellicle, which consists of cells and a self-produced matrix of cell-attached polysaccharide	175:337	the ability to grow floating on the surface of the medium by producing pellicle, which consists of cells and a self-produced matrix of cell-attached polysaccharide	175:337	Acetobacter species are well known to have the ability to grow floating on the surface of the medium by producing pellicle, which consists of cells and a self-produced matrix of cell-attached polysaccharide.
24559734	5	11	from	xylose	987:992	arg1	1:4:2					1017:1021	1:4:2	1017:1021	1:4:2	1017:1021	For example, the pellicle polysaccharide of SL13E-2 is composed of rhamnose and glucose in the ratio 1:8, and that of SL13E-4 and mesophilic A. pasteurianus NBRC3191 consists of rhamnose, glucose and xylose in the ratio 1:5:2 and 1:4:2, respectively.
24559734	5	11	from	xylose	987:992	arg1	1:5:2					1007:1011	the ratio 1:5:2	997:1011	the ratio 1:5:2	997:1011	For example, the pellicle polysaccharide of SL13E-2 is composed of rhamnose and glucose in the ratio 1:8, and that of SL13E-4 and mesophilic A. pasteurianus NBRC3191 consists of rhamnose, glucose and xylose in the ratio 1:5:2 and 1:4:2, respectively.
24559734	7	12	theme	poor	1354:1357	arg1	induction					1359:1367	poor induction	1354:1367	poor induction	1354:1367	The pellicle formation of thermotolerant SL13E-2, SL13E-3, and SL13E-4 was found to be significantly induced by the addition of ethanol, while poor induction was observed with SKU1108.
24559734	0	13	theme	patterns	122:129	arg1	characterization					61:76	characterization	61:76	characterization of the polysaccharides and of the induction patterns	61:129	Pellicle of thermotolerant Acetobacter pasteurianus strains: characterization of the polysaccharides and of the induction patterns.
24559734	8	14	theme	polysaccharide	1612:1625	arg1	molecule					1627:1634	the polysaccharide molecule	1608:1634	the polysaccharide molecule	1608:1634	The size and sugar composition of the polysaccharides obtained from cells induced by ethanol and by uninduced cells were the same, indicating that the number of molecules of the polysaccharides had increased but the polysaccharide molecule remained unchanged.
24559734	3	15	theme	pellicle	522:529	arg1	polysaccharides					531:545	The pellicle polysaccharides	518:545	The pellicle polysaccharides	518:545	The pellicle polysaccharides of these three strains and of A. pasteurianus SKU1108, which was originally isolated from Thailand, were characterized.
24559734	6	16	from	rhamnose	1133:1140	arg1	2:2:1					1178:1182	the ratio 2:2:1	1168:1182	the ratio 2:2:1	1168:1182	On the other hand, the pellicle polysaccharides of SL13E-3 and SKU1108 strains are composed of rhamnose, glucose and galactose in the ratio 2:2:1 and 1:5:2.5, respectively.
24559734	0	17	theme	induction	112:120	arg1	patterns					122:129	the induction patterns	108:129	the induction patterns	108:129	Pellicle of thermotolerant Acetobacter pasteurianus strains: characterization of the polysaccharides and of the induction patterns.
24559734	9	18	theme	pellicle	1741:1748	arg1	formation					1750:1758	pellicle formation	1741:1758	pellicle formation	1741:1758	The addition of a sugar source such as glucose, sucrose or fructose slightly induced pellicle formation in SKU1108, especially at 40°C.
24559734	5	19	from	glucose	975:981	arg1	1:4:2					1017:1021	1:4:2	1017:1021	1:4:2	1017:1021	For example, the pellicle polysaccharide of SL13E-2 is composed of rhamnose and glucose in the ratio 1:8, and that of SL13E-4 and mesophilic A. pasteurianus NBRC3191 consists of rhamnose, glucose and xylose in the ratio 1:5:2 and 1:4:2, respectively.
24559734	5	19	from	glucose	975:981	arg1	1:5:2					1007:1011	the ratio 1:5:2	997:1011	the ratio 1:5:2	997:1011	For example, the pellicle polysaccharide of SL13E-2 is composed of rhamnose and glucose in the ratio 1:8, and that of SL13E-4 and mesophilic A. pasteurianus NBRC3191 consists of rhamnose, glucose and xylose in the ratio 1:5:2 and 1:4:2, respectively.
24559734	6	20	theme	other	1045:1049	arg1	hand					1051:1054	the other hand	1041:1054	the other hand	1041:1054	On the other hand, the pellicle polysaccharides of SL13E-3 and SKU1108 strains are composed of rhamnose, glucose and galactose in the ratio 2:2:1 and 1:5:2.5, respectively.
24559734	2	21	theme	Lankan	433:438	arg1	vinegar					448:454	Sri Lankan coconut vinegar	429:454	Sri Lankan coconut vinegar	429:454	We previously isolated three thermotolerant strains (SL13E-2, SL13E-3, and SL13E-4) from Sri Lankan coconut vinegar and identified all these strains as Acetobacter pasteurianus.
24559734	4	22	theme	strains	755:761	arg1	polysaccharides					714:728	the pellicle polysaccharides	701:728	the pellicle polysaccharides of these A. pasteurianus strains	701:761	The monosaccharide composition of the pellicle polysaccharides of these A. pasteurianus strains was found to be varied.
24559734	6	23	from	glucose	1143:1149	arg1	2:2:1					1178:1182	the ratio 2:2:1	1168:1182	the ratio 2:2:1	1168:1182	On the other hand, the pellicle polysaccharides of SL13E-3 and SKU1108 strains are composed of rhamnose, glucose and galactose in the ratio 2:2:1 and 1:5:2.5, respectively.
24559734	4	24	theme	monosaccharide	671:684	arg1	composition					686:696	The monosaccharide composition	667:696	The monosaccharide composition of the pellicle polysaccharides of these A. pasteurianus strains	667:761	The monosaccharide composition of the pellicle polysaccharides of these A. pasteurianus strains was found to be varied.
24559734	4	24	theme	monosaccharide	671:684	arg1	varied					779:784	varied	779:784	varied	779:784	The monosaccharide composition of the pellicle polysaccharides of these A. pasteurianus strains was found to be varied.
24559734	2	25	theme	Sri	429:431	arg1	vinegar					448:454	Sri Lankan coconut vinegar	429:454	Sri Lankan coconut vinegar	429:454	We previously isolated three thermotolerant strains (SL13E-2, SL13E-3, and SL13E-4) from Sri Lankan coconut vinegar and identified all these strains as Acetobacter pasteurianus.
24559734	8	26	theme	uninduced	1496:1504	arg1	cells					1506:1510	uninduced cells	1496:1510	uninduced cells	1496:1510	The size and sugar composition of the polysaccharides obtained from cells induced by ethanol and by uninduced cells were the same, indicating that the number of molecules of the polysaccharides had increased but the polysaccharide molecule remained unchanged.
24559734	4	27	theme	pasteurianus	742:753	arg1	strains					755:761	these A. pasteurianus strains	733:761	these A. pasteurianus strains	733:761	The monosaccharide composition of the pellicle polysaccharides of these A. pasteurianus strains was found to be varied.
24559734	0	28	theme	Acetobacter	27:37	arg1	pasteurianus					39:50	thermotolerant Acetobacter pasteurianus	12:50	thermotolerant Acetobacter pasteurianus	12:50	Pellicle of thermotolerant Acetobacter pasteurianus strains: characterization of the polysaccharides and of the induction patterns.
24559734	0	29	theme	thermotolerant	12:25	arg1	pasteurianus					39:50	thermotolerant Acetobacter pasteurianus	12:50	thermotolerant Acetobacter pasteurianus	12:50	Pellicle of thermotolerant Acetobacter pasteurianus strains: characterization of the polysaccharides and of the induction patterns.
24559734	5	30	theme	A.	928:929	arg1	pasteurianus					931:942	SL13E-4 and mesophilic A. pasteurianus NBRC3191	905:951	SL13E-4 and mesophilic A. pasteurianus NBRC3191	905:951	For example, the pellicle polysaccharide of SL13E-2 is composed of rhamnose and glucose in the ratio 1:8, and that of SL13E-4 and mesophilic A. pasteurianus NBRC3191 consists of rhamnose, glucose and xylose in the ratio 1:5:2 and 1:4:2, respectively.
24559734	3	31	theme	strains	562:568	arg1	polysaccharides					531:545	The pellicle polysaccharides	518:545	The pellicle polysaccharides	518:545	The pellicle polysaccharides of these three strains and of A. pasteurianus SKU1108, which was originally isolated from Thailand, were characterized.
24559734	5	32	theme	ratio	882:886	arg1	1:8					888:890	the ratio 1:8	878:890	the ratio 1:8	878:890	For example, the pellicle polysaccharide of SL13E-2 is composed of rhamnose and glucose in the ratio 1:8, and that of SL13E-4 and mesophilic A. pasteurianus NBRC3191 consists of rhamnose, glucose and xylose in the ratio 1:5:2 and 1:4:2, respectively.
24559734	4	33	theme	polysaccharides	714:728	arg1	composition					686:696	The monosaccharide composition	667:696	The monosaccharide composition of the pellicle polysaccharides of these A. pasteurianus strains	667:761	The monosaccharide composition of the pellicle polysaccharides of these A. pasteurianus strains was found to be varied.
24559734	4	33	theme	polysaccharides	714:728	arg1	varied					779:784	varied	779:784	varied	779:784	The monosaccharide composition of the pellicle polysaccharides of these A. pasteurianus strains was found to be varied.
24559734	0	34	theme	pasteurianus	39:50	arg1	Pellicle					0:7	Pellicle	0:7	Pellicle of thermotolerant Acetobacter pasteurianus	0:50	Pellicle of thermotolerant Acetobacter pasteurianus strains: characterization of the polysaccharides and of the induction patterns.
24559734	5	35	theme	ratio	1001:1005	arg1	1:5:2					1007:1011	the ratio 1:5:2	997:1011	the ratio 1:5:2	997:1011	For example, the pellicle polysaccharide of SL13E-2 is composed of rhamnose and glucose in the ratio 1:8, and that of SL13E-4 and mesophilic A. pasteurianus NBRC3191 consists of rhamnose, glucose and xylose in the ratio 1:5:2 and 1:4:2, respectively.
24559734	2	36	theme	Acetobacter	492:502	arg1	strains					481:487	all these strains	471:487	all these strains	471:487	We previously isolated three thermotolerant strains (SL13E-2, SL13E-3, and SL13E-4) from Sri Lankan coconut vinegar and identified all these strains as Acetobacter pasteurianus.
24559734	2	36	theme	Acetobacter	492:502	arg1	pasteurianus					504:515	Acetobacter pasteurianus	492:515	Acetobacter pasteurianus	492:515	We previously isolated three thermotolerant strains (SL13E-2, SL13E-3, and SL13E-4) from Sri Lankan coconut vinegar and identified all these strains as Acetobacter pasteurianus.
24559734	1	37	theme	self-produced	286:298	arg1	matrix					300:305	a self-produced matrix	284:305	a self-produced matrix of cell-attached polysaccharide	284:337	Acetobacter species are well known to have the ability to grow floating on the surface of the medium by producing pellicle, which consists of cells and a self-produced matrix of cell-attached polysaccharide.
24559734	3	38	theme	A.	577:578	arg1	pasteurianus					580:591	A. pasteurianus SKU1108	577:599	A. pasteurianus SKU1108	577:599	The pellicle polysaccharides of these three strains and of A. pasteurianus SKU1108, which was originally isolated from Thailand, were characterized.
24559734	5	39	from	rhamnose	854:861	arg1	1:8					888:890	the ratio 1:8	878:890	the ratio 1:8	878:890	For example, the pellicle polysaccharide of SL13E-2 is composed of rhamnose and glucose in the ratio 1:8, and that of SL13E-4 and mesophilic A. pasteurianus NBRC3191 consists of rhamnose, glucose and xylose in the ratio 1:5:2 and 1:4:2, respectively.
24559734	7	40	theme	thermotolerant	1237:1250	arg1	SL13E-2					1252:1258	thermotolerant SL13E-2	1237:1258	thermotolerant SL13E-2	1237:1258	The pellicle formation of thermotolerant SL13E-2, SL13E-3, and SL13E-4 was found to be significantly induced by the addition of ethanol, while poor induction was observed with SKU1108.
24559734	2	41	theme	coconut	440:446	arg1	vinegar					448:454	Sri Lankan coconut vinegar	429:454	Sri Lankan coconut vinegar	429:454	We previously isolated three thermotolerant strains (SL13E-2, SL13E-3, and SL13E-4) from Sri Lankan coconut vinegar and identified all these strains as Acetobacter pasteurianus.
24559734	9	42	theme	sugar	1674:1678	arg1	source					1680:1685	a sugar source	1672:1685	a sugar source	1672:1685	The addition of a sugar source such as glucose, sucrose or fructose slightly induced pellicle formation in SKU1108, especially at 40°C.
24559734	3	43	theme	pasteurianus	580:591	arg1	polysaccharides					531:545	The pellicle polysaccharides	518:545	The pellicle polysaccharides	518:545	The pellicle polysaccharides of these three strains and of A. pasteurianus SKU1108, which was originally isolated from Thailand, were characterized.
24559734	1	44	theme	Acetobacter	132:142	arg1	species					144:150	Acetobacter species	132:150	Acetobacter species	132:150	Acetobacter species are well known to have the ability to grow floating on the surface of the medium by producing pellicle, which consists of cells and a self-produced matrix of cell-attached polysaccharide.
24559734	3	45	attach	isolated	623:630	arg1	Thailand					637:644	Thailand	637:644	Thailand	637:644	The pellicle polysaccharides of these three strains and of A. pasteurianus SKU1108, which was originally isolated from Thailand, were characterized.
24559734	3	45	attach	isolated	623:630	arg2	polysaccharides					531:545	The pellicle polysaccharides	518:545	The pellicle polysaccharides	518:545	The pellicle polysaccharides of these three strains and of A. pasteurianus SKU1108, which was originally isolated from Thailand, were characterized.
24559734	7	46	theme	pellicle	1215:1222	arg1	formation					1224:1232	The pellicle formation	1211:1232	The pellicle formation of thermotolerant SL13E-2, SL13E-3, and SL13E-4	1211:1280	The pellicle formation of thermotolerant SL13E-2, SL13E-3, and SL13E-4 was found to be significantly induced by the addition of ethanol, while poor induction was observed with SKU1108.
24559734	9	47	theme	source	1680:1685	arg1	addition					1660:1667	The addition	1656:1667	The addition of a sugar source such as glucose, sucrose or fructose	1656:1722	The addition of a sugar source such as glucose, sucrose or fructose slightly induced pellicle formation in SKU1108, especially at 40°C.
24559734	8	48	theme	sugar	1409:1413	arg1	size					1400:1403	size	1400:1403	size	1400:1403	The size and sugar composition of the polysaccharides obtained from cells induced by ethanol and by uninduced cells were the same, indicating that the number of molecules of the polysaccharides had increased but the polysaccharide molecule remained unchanged.
24559734	8	48	theme	sugar	1409:1413	arg1	composition					1415:1425	sugar composition	1409:1425	sugar composition	1409:1425	The size and sugar composition of the polysaccharides obtained from cells induced by ethanol and by uninduced cells were the same, indicating that the number of molecules of the polysaccharides had increased but the polysaccharide molecule remained unchanged.
24559734	8	48	theme	sugar	1409:1413	arg1	same					1521:1524	same	1521:1524	same	1521:1524	The size and sugar composition of the polysaccharides obtained from cells induced by ethanol and by uninduced cells were the same, indicating that the number of molecules of the polysaccharides had increased but the polysaccharide molecule remained unchanged.
24559734	6	49	theme	ratio	1172:1176	arg1	2:2:1					1178:1182	the ratio 2:2:1	1168:1182	the ratio 2:2:1	1168:1182	On the other hand, the pellicle polysaccharides of SL13E-3 and SKU1108 strains are composed of rhamnose, glucose and galactose in the ratio 2:2:1 and 1:5:2.5, respectively.
24559734	7	50	theme	ethanol	1339:1345	arg1	addition					1327:1334	the addition	1323:1334	the addition of ethanol	1323:1345	The pellicle formation of thermotolerant SL13E-2, SL13E-3, and SL13E-4 was found to be significantly induced by the addition of ethanol, while poor induction was observed with SKU1108.
24559734	4	51	theme	A.	739:740	arg1	strains					755:761	these A. pasteurianus strains	733:761	these A. pasteurianus strains	733:761	The monosaccharide composition of the pellicle polysaccharides of these A. pasteurianus strains was found to be varied.
24559734	7	52	theme	SL13E-2	1252:1258	arg1	formation					1224:1232	The pellicle formation	1211:1232	The pellicle formation of thermotolerant SL13E-2, SL13E-3, and SL13E-4	1211:1280	The pellicle formation of thermotolerant SL13E-2, SL13E-3, and SL13E-4 was found to be significantly induced by the addition of ethanol, while poor induction was observed with SKU1108.
24559734	2	53	dep	strains	384:390	arg1	SL13E-2					393:399	SL13E-2	393:399	SL13E-2	393:399	We previously isolated three thermotolerant strains (SL13E-2, SL13E-3, and SL13E-4) from Sri Lankan coconut vinegar and identified all these strains as Acetobacter pasteurianus.
24559734	2	53	dep	strains	384:390	arg1	strains					384:390	three thermotolerant strains	363:390	three thermotolerant strains (SL13E-2, SL13E-3, and SL13E-4)	363:422	We previously isolated three thermotolerant strains (SL13E-2, SL13E-3, and SL13E-4) from Sri Lankan coconut vinegar and identified all these strains as Acetobacter pasteurianus.
24559734	2	53	dep	strains	384:390	arg1	SL13E-3					402:408	SL13E-3	402:408	SL13E-3	402:408	We previously isolated three thermotolerant strains (SL13E-2, SL13E-3, and SL13E-4) from Sri Lankan coconut vinegar and identified all these strains as Acetobacter pasteurianus.
24559734	2	53	dep	strains	384:390	arg1	SL13E-4					415:421	SL13E-4	415:421	SL13E-4	415:421	We previously isolated three thermotolerant strains (SL13E-2, SL13E-3, and SL13E-4) from Sri Lankan coconut vinegar and identified all these strains as Acetobacter pasteurianus.
24559734	8	54	theme	polysaccharides	1434:1448	arg1	size					1400:1403	size	1400:1403	size	1400:1403	The size and sugar composition of the polysaccharides obtained from cells induced by ethanol and by uninduced cells were the same, indicating that the number of molecules of the polysaccharides had increased but the polysaccharide molecule remained unchanged.
24559734	8	54	theme	polysaccharides	1434:1448	arg1	composition					1415:1425	sugar composition	1409:1425	sugar composition	1409:1425	The size and sugar composition of the polysaccharides obtained from cells induced by ethanol and by uninduced cells were the same, indicating that the number of molecules of the polysaccharides had increased but the polysaccharide molecule remained unchanged.
24559734	8	54	theme	polysaccharides	1434:1448	arg1	same					1521:1524	same	1521:1524	same	1521:1524	The size and sugar composition of the polysaccharides obtained from cells induced by ethanol and by uninduced cells were the same, indicating that the number of molecules of the polysaccharides had increased but the polysaccharide molecule remained unchanged.
24559734	6	55	theme	pellicle	1061:1068	arg1	polysaccharides					1070:1084	the pellicle polysaccharides	1057:1084	the pellicle polysaccharides of SL13E-3 and SKU1108 strains	1057:1115	On the other hand, the pellicle polysaccharides of SL13E-3 and SKU1108 strains are composed of rhamnose, glucose and galactose in the ratio 2:2:1 and 1:5:2.5, respectively.
24559734	7	56	theme	SL13E-4	1274:1280	arg1	formation					1224:1232	The pellicle formation	1211:1232	The pellicle formation of thermotolerant SL13E-2, SL13E-3, and SL13E-4	1211:1280	The pellicle formation of thermotolerant SL13E-2, SL13E-3, and SL13E-4 was found to be significantly induced by the addition of ethanol, while poor induction was observed with SKU1108.
24559734	5	57	theme	SL13E-4	905:911	arg1	pasteurianus					931:942	SL13E-4 and mesophilic A. pasteurianus NBRC3191	905:951	SL13E-4 and mesophilic A. pasteurianus NBRC3191	905:951	For example, the pellicle polysaccharide of SL13E-2 is composed of rhamnose and glucose in the ratio 1:8, and that of SL13E-4 and mesophilic A. pasteurianus NBRC3191 consists of rhamnose, glucose and xylose in the ratio 1:5:2 and 1:4:2, respectively.
24559734	7	58	theme	SL13E-3	1261:1267	arg1	formation					1224:1232	The pellicle formation	1211:1232	The pellicle formation of thermotolerant SL13E-2, SL13E-3, and SL13E-4	1211:1280	The pellicle formation of thermotolerant SL13E-2, SL13E-3, and SL13E-4 was found to be significantly induced by the addition of ethanol, while poor induction was observed with SKU1108.
24559734	2	59	theme	thermotolerant	369:382	arg1	SL13E-2					393:399	SL13E-2	393:399	SL13E-2	393:399	We previously isolated three thermotolerant strains (SL13E-2, SL13E-3, and SL13E-4) from Sri Lankan coconut vinegar and identified all these strains as Acetobacter pasteurianus.
24559734	2	59	theme	thermotolerant	369:382	arg1	strains					384:390	three thermotolerant strains	363:390	three thermotolerant strains (SL13E-2, SL13E-3, and SL13E-4)	363:422	We previously isolated three thermotolerant strains (SL13E-2, SL13E-3, and SL13E-4) from Sri Lankan coconut vinegar and identified all these strains as Acetobacter pasteurianus.
24559734	2	59	theme	thermotolerant	369:382	arg1	SL13E-3					402:408	SL13E-3	402:408	SL13E-3	402:408	We previously isolated three thermotolerant strains (SL13E-2, SL13E-3, and SL13E-4) from Sri Lankan coconut vinegar and identified all these strains as Acetobacter pasteurianus.
24559734	2	59	theme	thermotolerant	369:382	arg1	SL13E-4					415:421	SL13E-4	415:421	SL13E-4	415:421	We previously isolated three thermotolerant strains (SL13E-2, SL13E-3, and SL13E-4) from Sri Lankan coconut vinegar and identified all these strains as Acetobacter pasteurianus.
24559734	6	60	from	1:5:2.5	1188:1194	arg1	2:2:1					1178:1182	the ratio 2:2:1	1168:1182	the ratio 2:2:1	1168:1182	On the other hand, the pellicle polysaccharides of SL13E-3 and SKU1108 strains are composed of rhamnose, glucose and galactose in the ratio 2:2:1 and 1:5:2.5, respectively.
24559734	1	61	theme	medium	226:231	arg1	surface					211:217	the surface	207:217	the surface of the medium	207:231	Acetobacter species are well known to have the ability to grow floating on the surface of the medium by producing pellicle, which consists of cells and a self-produced matrix of cell-attached polysaccharide.
24559734	1	62	theme	cell-attached	310:322	arg1	polysaccharide					324:337	cell-attached polysaccharide	310:337	cell-attached polysaccharide	310:337	Acetobacter species are well known to have the ability to grow floating on the surface of the medium by producing pellicle, which consists of cells and a self-produced matrix of cell-attached polysaccharide.
24559734	5	63	theme	SL13E-2	831:837	arg1	polysaccharide					813:826	the pellicle polysaccharide	800:826	the pellicle polysaccharide of SL13E-2	800:837	For example, the pellicle polysaccharide of SL13E-2 is composed of rhamnose and glucose in the ratio 1:8, and that of SL13E-4 and mesophilic A. pasteurianus NBRC3191 consists of rhamnose, glucose and xylose in the ratio 1:5:2 and 1:4:2, respectively.
24559734	5	64	theme	pellicle	804:811	arg1	polysaccharide					813:826	the pellicle polysaccharide	800:826	the pellicle polysaccharide of SL13E-2	800:837	For example, the pellicle polysaccharide of SL13E-2 is composed of rhamnose and glucose in the ratio 1:8, and that of SL13E-4 and mesophilic A. pasteurianus NBRC3191 consists of rhamnose, glucose and xylose in the ratio 1:5:2 and 1:4:2, respectively.
25977013	1	0	from	microencapsulation	174:191	arg1	fruit					218:222	cactus fruit	211:222	cactus fruit	211:222	The microencapsulation of betalains from cactus fruit by spray drying was evaluated as a stabilization strategy for these pigments.
25977013	2	1	theme	purple	357:362	arg1	fruits					364:369	purple fruits	357:369	purple fruits of Opuntia ficus-indica (BE)	357:398	The betalains used as active agent were extracted from purple fruits of Opuntia ficus-indica (BE) and encapsulated with maltodextrin and cladode mucilage MD-CM and only with MD.
25977013	5	2	theme	different	873:881	arg1	humidities					892:901	different relative humidities	873:901	different relative humidities	873:901	A pigment storage stability test was performed at 18 °C and different relative humidities.
25977013	3	3	theme	dietary	658:664	arg1	content					672:678	dietary fiber content	658:678	dietary fiber content	658:678	The microcapsulates were characterized by scanning electron microscopy (SEM), thermal analysis (TGA-DSC), tristimulus colorimetry, as well as, their humidity, water activity and dietary fiber content were also determined.
25977013	1	4	theme	stabilization	259:271	arg1	microencapsulation					174:191	The microencapsulation	170:191	The microencapsulation of betalains from cactus fruit by spray drying	170:238	The microencapsulation of betalains from cactus fruit by spray drying was evaluated as a stabilization strategy for these pigments.
25977013	1	4	theme	stabilization	259:271	arg1	strategy					273:280	a stabilization strategy	257:280	a stabilization strategy for these pigments	257:299	The microencapsulation of betalains from cactus fruit by spray drying was evaluated as a stabilization strategy for these pigments.
25977013	0	5	theme	spray	85:89	arg1	drying					91:96	spray drying	85:96	spray drying using cactus cladode mucilage and maltodextrin as encapsulating agents	85:167	Microencapsulation of betalains obtained from cactus fruit (Opuntia ficus-indica) by spray drying using cactus cladode mucilage and maltodextrin as encapsulating agents.
25977013	6	6	theme	encapsulation	956:968	arg1	efficiency					970:979	the encapsulation efficiency	952:979	the encapsulation efficiency	952:979	The addition of CM in the formulation increased the encapsulation efficiency, diminished the moisture content, and allowed to obtain more uniform size and spherical particles, with high dietary fiber content.
25977013	0	7	theme	cactus	104:109	arg1	maltodextrin					132:143	maltodextrin	132:143	maltodextrin	132:143	Microencapsulation of betalains obtained from cactus fruit (Opuntia ficus-indica) by spray drying using cactus cladode mucilage and maltodextrin as encapsulating agents.
25977013	0	7	theme	cactus	104:109	arg1	agents					162:167	encapsulating agents	148:167	encapsulating agents	148:167	Microencapsulation of betalains obtained from cactus fruit (Opuntia ficus-indica) by spray drying using cactus cladode mucilage and maltodextrin as encapsulating agents.
25977013	0	7	theme	cactus	104:109	arg1	mucilage					119:126	cactus cladode mucilage	104:126	cactus cladode mucilage	104:126	Microencapsulation of betalains obtained from cactus fruit (Opuntia ficus-indica) by spray drying using cactus cladode mucilage and maltodextrin as encapsulating agents.
25977013	5	8	theme	pigment	815:821	arg1	test					841:844	A pigment storage stability test	813:844	A pigment storage stability test	813:844	A pigment storage stability test was performed at 18 °C and different relative humidities.
25977013	4	9	theme	active	706:711	arg1	content					719:725	The active agent content	702:725	The active agent content	702:725	The active agent content was measured by UV-Vis spectrophotometry and its composition confirmed by HPLC-ESIMS.
25977013	3	10	theme	electron	531:538	arg1	colorimetry					598:608	tristimulus colorimetry	586:608	tristimulus colorimetry	586:608	The microcapsulates were characterized by scanning electron microscopy (SEM), thermal analysis (TGA-DSC), tristimulus colorimetry, as well as, their humidity, water activity and dietary fiber content were also determined.
25977013	3	10	theme	electron	531:538	arg1	SEM					552:554	SEM	552:554	SEM	552:554	The microcapsulates were characterized by scanning electron microscopy (SEM), thermal analysis (TGA-DSC), tristimulus colorimetry, as well as, their humidity, water activity and dietary fiber content were also determined.
25977013	3	10	theme	electron	531:538	arg1	microscopy					540:549	scanning electron microscopy	522:549	scanning electron microscopy (SEM)	522:555	The microcapsulates were characterized by scanning electron microscopy (SEM), thermal analysis (TGA-DSC), tristimulus colorimetry, as well as, their humidity, water activity and dietary fiber content were also determined.
25977013	3	10	theme	electron	531:538	arg1	analysis					566:573	thermal analysis	558:573	thermal analysis (TGA-DSC)	558:583	The microcapsulates were characterized by scanning electron microscopy (SEM), thermal analysis (TGA-DSC), tristimulus colorimetry, as well as, their humidity, water activity and dietary fiber content were also determined.
25977013	6	11	theme	uniform	1042:1048	arg1	size					1050:1053	more uniform size	1037:1053	more uniform size	1037:1053	The addition of CM in the formulation increased the encapsulation efficiency, diminished the moisture content, and allowed to obtain more uniform size and spherical particles, with high dietary fiber content.
25977013	6	12	theme	spherical	1059:1067	arg1	particles					1069:1077	spherical particles	1059:1077	spherical particles	1059:1077	The addition of CM in the formulation increased the encapsulation efficiency, diminished the moisture content, and allowed to obtain more uniform size and spherical particles, with high dietary fiber content.
25977013	2	13	theme	mucilage	447:454	arg1	MD-CM					456:460	cladode mucilage MD-CM	439:460	cladode mucilage MD-CM	439:460	The betalains used as active agent were extracted from purple fruits of Opuntia ficus-indica (BE) and encapsulated with maltodextrin and cladode mucilage MD-CM and only with MD.
25977013	3	14	theme	thermal	558:564	arg1	microscopy					540:549	scanning electron microscopy	522:549	scanning electron microscopy (SEM)	522:555	The microcapsulates were characterized by scanning electron microscopy (SEM), thermal analysis (TGA-DSC), tristimulus colorimetry, as well as, their humidity, water activity and dietary fiber content were also determined.
25977013	3	14	theme	thermal	558:564	arg1	TGA-DSC					576:582	TGA-DSC	576:582	TGA-DSC	576:582	The microcapsulates were characterized by scanning electron microscopy (SEM), thermal analysis (TGA-DSC), tristimulus colorimetry, as well as, their humidity, water activity and dietary fiber content were also determined.
25977013	3	14	theme	thermal	558:564	arg1	analysis					566:573	thermal analysis	558:573	thermal analysis (TGA-DSC)	558:583	The microcapsulates were characterized by scanning electron microscopy (SEM), thermal analysis (TGA-DSC), tristimulus colorimetry, as well as, their humidity, water activity and dietary fiber content were also determined.
25977013	2	15	theme	ficus-indica	382:393	arg1	fruits					364:369	purple fruits	357:369	purple fruits of Opuntia ficus-indica (BE)	357:398	The betalains used as active agent were extracted from purple fruits of Opuntia ficus-indica (BE) and encapsulated with maltodextrin and cladode mucilage MD-CM and only with MD.
25977013	2	16	theme	cladode	439:445	arg1	MD-CM					456:460	cladode mucilage MD-CM	439:460	cladode mucilage MD-CM	439:460	The betalains used as active agent were extracted from purple fruits of Opuntia ficus-indica (BE) and encapsulated with maltodextrin and cladode mucilage MD-CM and only with MD.
25977013	7	17	from	colorant	1193:1200	arg1	industry					1214:1221	the food industry	1205:1221	the food industry	1205:1221	These microencapsulates are promising functional additive to be used as natural colorant in the food industry.
25977013	1	18	theme	betalains	196:204	arg1	microencapsulation					174:191	The microencapsulation	170:191	The microencapsulation of betalains from cactus fruit by spray drying	170:238	The microencapsulation of betalains from cactus fruit by spray drying was evaluated as a stabilization strategy for these pigments.
25977013	1	18	theme	betalains	196:204	arg1	strategy					273:280	a stabilization strategy	257:280	a stabilization strategy for these pigments	257:299	The microencapsulation of betalains from cactus fruit by spray drying was evaluated as a stabilization strategy for these pigments.
25977013	0	19	theme	betalains	22:30	arg1	Microencapsulation					0:17	Microencapsulation	0:17	Microencapsulation of betalains	0:30	Microencapsulation of betalains obtained from cactus fruit (Opuntia ficus-indica) by spray drying using cactus cladode mucilage and maltodextrin as encapsulating agents.
25977013	0	20	theme	cladode	111:117	arg1	maltodextrin					132:143	maltodextrin	132:143	maltodextrin	132:143	Microencapsulation of betalains obtained from cactus fruit (Opuntia ficus-indica) by spray drying using cactus cladode mucilage and maltodextrin as encapsulating agents.
25977013	0	20	theme	cladode	111:117	arg1	agents					162:167	encapsulating agents	148:167	encapsulating agents	148:167	Microencapsulation of betalains obtained from cactus fruit (Opuntia ficus-indica) by spray drying using cactus cladode mucilage and maltodextrin as encapsulating agents.
25977013	0	20	theme	cladode	111:117	arg1	mucilage					119:126	cactus cladode mucilage	104:126	cactus cladode mucilage	104:126	Microencapsulation of betalains obtained from cactus fruit (Opuntia ficus-indica) by spray drying using cactus cladode mucilage and maltodextrin as encapsulating agents.
25977013	4	21	theme	agent	713:717	arg1	content					719:725	The active agent content	702:725	The active agent content	702:725	The active agent content was measured by UV-Vis spectrophotometry and its composition confirmed by HPLC-ESIMS.
25977013	1	22	theme	cactus	211:216	arg1	fruit					218:222	cactus fruit	211:222	cactus fruit	211:222	The microencapsulation of betalains from cactus fruit by spray drying was evaluated as a stabilization strategy for these pigments.
25977013	0	23	theme	cactus	46:51	arg1	fruit					53:57	cactus fruit	46:57	cactus fruit (Opuntia ficus-indica)	46:80	Microencapsulation of betalains obtained from cactus fruit (Opuntia ficus-indica) by spray drying using cactus cladode mucilage and maltodextrin as encapsulating agents.
25977013	5	24	theme	storage	823:829	arg1	test					841:844	A pigment storage stability test	813:844	A pigment storage stability test	813:844	A pigment storage stability test was performed at 18 °C and different relative humidities.
25977013	0	25	dep	fruit	53:57	arg1	ficus-indica					68:79	Opuntia ficus-indica	60:79	Opuntia ficus-indica	60:79	Microencapsulation of betalains obtained from cactus fruit (Opuntia ficus-indica) by spray drying using cactus cladode mucilage and maltodextrin as encapsulating agents.
25977013	1	26	from	fruit	218:222	arg1	betalains					196:204	betalains	196:204	betalains from cactus fruit	196:222	The microencapsulation of betalains from cactus fruit by spray drying was evaluated as a stabilization strategy for these pigments.
25977013	1	26	from	fruit	218:222	arg1	microencapsulation					174:191	The microencapsulation	170:191	The microencapsulation of betalains from cactus fruit by spray drying	170:238	The microencapsulation of betalains from cactus fruit by spray drying was evaluated as a stabilization strategy for these pigments.
25977013	1	26	from	fruit	218:222	arg1	strategy					273:280	a stabilization strategy	257:280	a stabilization strategy for these pigments	257:299	The microencapsulation of betalains from cactus fruit by spray drying was evaluated as a stabilization strategy for these pigments.
25977013	5	27	theme	stability	831:839	arg1	test					841:844	A pigment storage stability test	813:844	A pigment storage stability test	813:844	A pigment storage stability test was performed at 18 °C and different relative humidities.
25977013	6	28	theme	CM	920:921	arg1	addition					908:915	The addition	904:915	The addition of CM in the formulation	904:940	The addition of CM in the formulation increased the encapsulation efficiency, diminished the moisture content, and allowed to obtain more uniform size and spherical particles, with high dietary fiber content.
25977013	0	29	theme	encapsulating	148:160	arg1	maltodextrin					132:143	maltodextrin	132:143	maltodextrin	132:143	Microencapsulation of betalains obtained from cactus fruit (Opuntia ficus-indica) by spray drying using cactus cladode mucilage and maltodextrin as encapsulating agents.
25977013	0	29	theme	encapsulating	148:160	arg1	agents					162:167	encapsulating agents	148:167	encapsulating agents	148:167	Microencapsulation of betalains obtained from cactus fruit (Opuntia ficus-indica) by spray drying using cactus cladode mucilage and maltodextrin as encapsulating agents.
25977013	0	29	theme	encapsulating	148:160	arg1	mucilage					119:126	cactus cladode mucilage	104:126	cactus cladode mucilage	104:126	Microencapsulation of betalains obtained from cactus fruit (Opuntia ficus-indica) by spray drying using cactus cladode mucilage and maltodextrin as encapsulating agents.
25977013	5	30	theme	relative	883:890	arg1	humidities					892:901	different relative humidities	873:901	different relative humidities	873:901	A pigment storage stability test was performed at 18 °C and different relative humidities.
25977013	6	31	from	addition	908:915	arg1	formulation					930:940	the formulation	926:940	the formulation	926:940	The addition of CM in the formulation increased the encapsulation efficiency, diminished the moisture content, and allowed to obtain more uniform size and spherical particles, with high dietary fiber content.
25977013	3	32	theme	water	639:643	arg1	humidity					629:636	their humidity	623:636	their humidity	623:636	The microcapsulates were characterized by scanning electron microscopy (SEM), thermal analysis (TGA-DSC), tristimulus colorimetry, as well as, their humidity, water activity and dietary fiber content were also determined.
25977013	3	32	theme	water	639:643	arg1	activity					645:652	water activity	639:652	water activity	639:652	The microcapsulates were characterized by scanning electron microscopy (SEM), thermal analysis (TGA-DSC), tristimulus colorimetry, as well as, their humidity, water activity and dietary fiber content were also determined.
25977013	6	33	theme	fiber	1098:1102	arg1	content					1104:1110	high dietary fiber content	1085:1110	high dietary fiber content	1085:1110	The addition of CM in the formulation increased the encapsulation efficiency, diminished the moisture content, and allowed to obtain more uniform size and spherical particles, with high dietary fiber content.
25977013	2	34	dep	ficus-indica	382:393	arg1	BE					396:397	BE	396:397	BE	396:397	The betalains used as active agent were extracted from purple fruits of Opuntia ficus-indica (BE) and encapsulated with maltodextrin and cladode mucilage MD-CM and only with MD.
25977013	6	35	theme	dietary	1090:1096	arg1	content					1104:1110	high dietary fiber content	1085:1110	high dietary fiber content	1085:1110	The addition of CM in the formulation increased the encapsulation efficiency, diminished the moisture content, and allowed to obtain more uniform size and spherical particles, with high dietary fiber content.
25977013	6	36	theme	high	1085:1088	arg1	content					1104:1110	high dietary fiber content	1085:1110	high dietary fiber content	1085:1110	The addition of CM in the formulation increased the encapsulation efficiency, diminished the moisture content, and allowed to obtain more uniform size and spherical particles, with high dietary fiber content.
25977013	1	37	theme	spray	227:231	arg1	drying					233:238	spray drying	227:238	spray drying	227:238	The microencapsulation of betalains from cactus fruit by spray drying was evaluated as a stabilization strategy for these pigments.
25977013	4	38	theme	UV-Vis	743:748	arg1	spectrophotometry					750:766	UV-Vis spectrophotometry	743:766	UV-Vis spectrophotometry	743:766	The active agent content was measured by UV-Vis spectrophotometry and its composition confirmed by HPLC-ESIMS.
25977013	2	39	theme	active	324:329	arg1	agent					331:335	active agent	324:335	active agent	324:335	The betalains used as active agent were extracted from purple fruits of Opuntia ficus-indica (BE) and encapsulated with maltodextrin and cladode mucilage MD-CM and only with MD.
25977013	3	40	theme	tristimulus	586:596	arg1	colorimetry					598:608	tristimulus colorimetry	586:608	tristimulus colorimetry	586:608	The microcapsulates were characterized by scanning electron microscopy (SEM), thermal analysis (TGA-DSC), tristimulus colorimetry, as well as, their humidity, water activity and dietary fiber content were also determined.
25977013	3	40	theme	tristimulus	586:596	arg1	microscopy					540:549	scanning electron microscopy	522:549	scanning electron microscopy (SEM)	522:555	The microcapsulates were characterized by scanning electron microscopy (SEM), thermal analysis (TGA-DSC), tristimulus colorimetry, as well as, their humidity, water activity and dietary fiber content were also determined.
25977013	6	41	theme	moisture	997:1004	arg1	content					1006:1012	the moisture content	993:1012	the moisture content	993:1012	The addition of CM in the formulation increased the encapsulation efficiency, diminished the moisture content, and allowed to obtain more uniform size and spherical particles, with high dietary fiber content.
25977013	7	42	theme	food	1209:1212	arg1	industry					1214:1221	the food industry	1205:1221	the food industry	1205:1221	These microencapsulates are promising functional additive to be used as natural colorant in the food industry.
25977013	3	43	theme	fiber	666:670	arg1	content					672:678	dietary fiber content	658:678	dietary fiber content	658:678	The microcapsulates were characterized by scanning electron microscopy (SEM), thermal analysis (TGA-DSC), tristimulus colorimetry, as well as, their humidity, water activity and dietary fiber content were also determined.
25977013	3	44	theme	scanning	522:529	arg1	colorimetry					598:608	tristimulus colorimetry	586:608	tristimulus colorimetry	586:608	The microcapsulates were characterized by scanning electron microscopy (SEM), thermal analysis (TGA-DSC), tristimulus colorimetry, as well as, their humidity, water activity and dietary fiber content were also determined.
25977013	3	44	theme	scanning	522:529	arg1	SEM					552:554	SEM	552:554	SEM	552:554	The microcapsulates were characterized by scanning electron microscopy (SEM), thermal analysis (TGA-DSC), tristimulus colorimetry, as well as, their humidity, water activity and dietary fiber content were also determined.
25977013	3	44	theme	scanning	522:529	arg1	microscopy					540:549	scanning electron microscopy	522:549	scanning electron microscopy (SEM)	522:555	The microcapsulates were characterized by scanning electron microscopy (SEM), thermal analysis (TGA-DSC), tristimulus colorimetry, as well as, their humidity, water activity and dietary fiber content were also determined.
25977013	3	44	theme	scanning	522:529	arg1	analysis					566:573	thermal analysis	558:573	thermal analysis (TGA-DSC)	558:583	The microcapsulates were characterized by scanning electron microscopy (SEM), thermal analysis (TGA-DSC), tristimulus colorimetry, as well as, their humidity, water activity and dietary fiber content were also determined.
25977013	7	45	theme	natural	1185:1191	arg1	colorant					1193:1200	natural colorant	1185:1200	natural colorant in the food industry	1185:1221	These microencapsulates are promising functional additive to be used as natural colorant in the food industry.
25448110	6	0	theme	scanning	1103:1110	arg1	calorimetry					1112:1122	Differential scanning calorimetry	1090:1122	Differential scanning calorimetry	1090:1122	Differential scanning calorimetry and circular dichroism data revealed that the CRM197 structure was altered by the chemical conjugation, while DT and the formaldehyde-treated form of CRM197 were less impacted, depending on the degree of glycosylation.
25448110	3	1	theme	several	502:508	arg1	vaccines					510:517	several vaccines	502:517	several vaccines	502:517	Modern childhood routine vaccination schedules include the administration of several vaccines simultaneously or in close sequence, increasing the concern that the repeated exposure to conjugates based on these carrier proteins might interfere with the anti-polysaccharide response.
25448110	7	2	theme	anti-carrier	1487:1498	arg1	antibodies					1500:1509	anti-carrier antibodies	1487:1509	anti-carrier antibodies	1487:1509	Investigating the binding and avidity properties of IgGs induced in mice by non-conjugated carriers, we found that CRM197 induced low levels of anti-carrier antibodies, with decreased avidity for its MenA conjugates and poor binding to DT and respective MenA conjugates.
25448110	8	3	theme	antibody	1643:1650	arg1	titers					1652:1657	high antibody titers	1638:1657	high antibody titers able to bind with comparable avidity both the protein and its conjugates but showing very low avidity for CRM197 and related conjugates	1638:1793	In contrast, DT induced high antibody titers able to bind with comparable avidity both the protein and its conjugates but showing very low avidity for CRM197 and related conjugates.
25448110	9	4	theme	priming	2142:2148	arg1	context					2150:2156	a carrier priming context	2132:2156	a carrier priming context	2132:2156	The low intrinsic immunogenicity of CRM197 as compared to DT, the structural modifications induced by glycoconjugation and detoxification processes, resulting in conformational changes in CRM197 and DT epitopes with consequent alteration of the antibody recognition and avidity, might explain the different behavior of CRM197 and DT in a carrier priming context.
25448110	2	5	theme	commercial	292:301	arg1	vaccines					313:320	commercial conjugate vaccines	292:320	commercial conjugate vaccines	292:320	The main carrier proteins used in commercial conjugate vaccines are the non-toxic mutant of diphtheria toxin (CRM197), diphtheria toxoid (DT) and tetanus toxoid (TT).
25448110	4	6	theme	CRM197	849:854	arg1	MenA					883:886	MenA	883:886	MenA	883:886	Extending previous observations we show here that priming mice with CRM197 or DT does not suppress the response to the carbohydrate moiety of CRM197 meningococcal serogroup A (MenA) conjugates, while priming with DT can suppress the response to DT-MenA conjugates.
25448110	4	6	theme	CRM197	849:854	arg1	A					880:880	CRM197 meningococcal serogroup A	849:880	CRM197 meningococcal serogroup A (MenA) conjugates	849:898	Extending previous observations we show here that priming mice with CRM197 or DT does not suppress the response to the carbohydrate moiety of CRM197 meningococcal serogroup A (MenA) conjugates, while priming with DT can suppress the response to DT-MenA conjugates.
25448110	6	7	theme	CRM197	1274:1279	arg1	impacted					1291:1298	impacted	1291:1298	impacted	1291:1298	Differential scanning calorimetry and circular dichroism data revealed that the CRM197 structure was altered by the chemical conjugation, while DT and the formaldehyde-treated form of CRM197 were less impacted, depending on the degree of glycosylation.
25448110	6	7	theme	CRM197	1274:1279	arg1	form					1266:1269	the formaldehyde-treated form	1241:1269	the formaldehyde-treated form of CRM197	1241:1279	Differential scanning calorimetry and circular dichroism data revealed that the CRM197 structure was altered by the chemical conjugation, while DT and the formaldehyde-treated form of CRM197 were less impacted, depending on the degree of glycosylation.
25448110	6	7	theme	CRM197	1274:1279	arg1	DT					1234:1235	DT	1234:1235	DT	1234:1235	Differential scanning calorimetry and circular dichroism data revealed that the CRM197 structure was altered by the chemical conjugation, while DT and the formaldehyde-treated form of CRM197 were less impacted, depending on the degree of glycosylation.
25448110	1	8	theme	Glycoconjugate	177:190	arg1	vaccines					192:199	Glycoconjugate vaccines	177:199	Glycoconjugate vaccines	177:199	Glycoconjugate vaccines play an enormous role in preventing infectious diseases.
25448110	4	9	theme	serogroup	870:878	arg1	MenA					883:886	MenA	883:886	MenA	883:886	Extending previous observations we show here that priming mice with CRM197 or DT does not suppress the response to the carbohydrate moiety of CRM197 meningococcal serogroup A (MenA) conjugates, while priming with DT can suppress the response to DT-MenA conjugates.
25448110	4	9	theme	serogroup	870:878	arg1	A					880:880	CRM197 meningococcal serogroup A	849:880	CRM197 meningococcal serogroup A (MenA) conjugates	849:898	Extending previous observations we show here that priming mice with CRM197 or DT does not suppress the response to the carbohydrate moiety of CRM197 meningococcal serogroup A (MenA) conjugates, while priming with DT can suppress the response to DT-MenA conjugates.
25448110	7	10	theme	non-conjugated	1419:1432	arg1	carriers					1434:1441	non-conjugated carriers	1419:1441	non-conjugated carriers	1419:1441	Investigating the binding and avidity properties of IgGs induced in mice by non-conjugated carriers, we found that CRM197 induced low levels of anti-carrier antibodies, with decreased avidity for its MenA conjugates and poor binding to DT and respective MenA conjugates.
25448110	9	11	theme	avidity	2066:2072	arg1	alteration					2023:2032	consequent alteration	2012:2032	consequent alteration of the antibody recognition and avidity	2012:2072	The low intrinsic immunogenicity of CRM197 as compared to DT, the structural modifications induced by glycoconjugation and detoxification processes, resulting in conformational changes in CRM197 and DT epitopes with consequent alteration of the antibody recognition and avidity, might explain the different behavior of CRM197 and DT in a carrier priming context.
25448110	1	12	theme	enormous	209:216	arg1	role					218:221	an enormous role	206:221	an enormous role	206:221	Glycoconjugate vaccines play an enormous role in preventing infectious diseases.
25448110	6	13	theme	formaldehyde-treated	1245:1264	arg1	impacted					1291:1298	impacted	1291:1298	impacted	1291:1298	Differential scanning calorimetry and circular dichroism data revealed that the CRM197 structure was altered by the chemical conjugation, while DT and the formaldehyde-treated form of CRM197 were less impacted, depending on the degree of glycosylation.
25448110	6	13	theme	formaldehyde-treated	1245:1264	arg1	form					1266:1269	the formaldehyde-treated form	1241:1269	the formaldehyde-treated form of CRM197	1241:1279	Differential scanning calorimetry and circular dichroism data revealed that the CRM197 structure was altered by the chemical conjugation, while DT and the formaldehyde-treated form of CRM197 were less impacted, depending on the degree of glycosylation.
25448110	9	14	theme	conformational	1958:1971	arg1	changes					1973:1979	conformational changes	1958:1979	conformational changes in CRM197 and DT epitopes with consequent alteration of the antibody recognition and avidity	1958:2072	The low intrinsic immunogenicity of CRM197 as compared to DT, the structural modifications induced by glycoconjugation and detoxification processes, resulting in conformational changes in CRM197 and DT epitopes with consequent alteration of the antibody recognition and avidity, might explain the different behavior of CRM197 and DT in a carrier priming context.
25448110	0	15	theme	glycoconjugates	113:127	arg1	immunogenicity					80:93	the immunogenicity	76:93	the immunogenicity of the respective glycoconjugates: biophysical and immunochemical interpretation	76:174	Carrier priming with CRM 197 or diphtheria toxoid has a different impact on the immunogenicity of the respective glycoconjugates: biophysical and immunochemical interpretation.
25448110	0	16	theme	biophysical	130:140	arg1	interpretation					161:174	interpretation	161:174	the respective glycoconjugates: biophysical and immunochemical interpretation	98:174	Carrier priming with CRM 197 or diphtheria toxoid has a different impact on the immunogenicity of the respective glycoconjugates: biophysical and immunochemical interpretation.
25448110	5	17	theme	immunochemical	1029:1042	arg1	techniques					1044:1053	biophysical and immunochemical techniques	1013:1053	biophysical and immunochemical techniques applied mainly to MenA conjugates	1013:1087	To explain these findings we made use of biophysical and immunochemical techniques applied mainly to MenA conjugates.
25448110	0	18	theme	immunochemical	146:159	arg1	interpretation					161:174	interpretation	161:174	the respective glycoconjugates: biophysical and immunochemical interpretation	98:174	Carrier priming with CRM 197 or diphtheria toxoid has a different impact on the immunogenicity of the respective glycoconjugates: biophysical and immunochemical interpretation.
25448110	2	19	theme	carrier	267:273	arg1	mutant					340:345	the non-toxic mutant	326:345	the non-toxic mutant of diphtheria toxin (CRM197), diphtheria toxoid (DT) and tetanus toxoid (TT)	326:422	The main carrier proteins used in commercial conjugate vaccines are the non-toxic mutant of diphtheria toxin (CRM197), diphtheria toxoid (DT) and tetanus toxoid (TT).
25448110	2	19	theme	carrier	267:273	arg1	proteins					275:282	The main carrier proteins	258:282	The main carrier proteins used in commercial conjugate vaccines	258:320	The main carrier proteins used in commercial conjugate vaccines are the non-toxic mutant of diphtheria toxin (CRM197), diphtheria toxoid (DT) and tetanus toxoid (TT).
25448110	8	20	theme	related	1776:1782	arg1	conjugates					1784:1793	related conjugates	1776:1793	related conjugates	1776:1793	In contrast, DT induced high antibody titers able to bind with comparable avidity both the protein and its conjugates but showing very low avidity for CRM197 and related conjugates.
25448110	3	21	theme	routine	442:448	arg1	schedules					462:470	Modern childhood routine vaccination schedules	425:470	Modern childhood routine vaccination schedules	425:470	Modern childhood routine vaccination schedules include the administration of several vaccines simultaneously or in close sequence, increasing the concern that the repeated exposure to conjugates based on these carrier proteins might interfere with the anti-polysaccharide response.
25448110	3	22	theme	close	540:544	arg1	sequence					546:553	close sequence	540:553	close sequence	540:553	Modern childhood routine vaccination schedules include the administration of several vaccines simultaneously or in close sequence, increasing the concern that the repeated exposure to conjugates based on these carrier proteins might interfere with the anti-polysaccharide response.
25448110	0	23	dep	glycoconjugates	113:127	arg1	interpretation					161:174	interpretation	161:174	the respective glycoconjugates: biophysical and immunochemical interpretation	98:174	Carrier priming with CRM 197 or diphtheria toxoid has a different impact on the immunogenicity of the respective glycoconjugates: biophysical and immunochemical interpretation.
25448110	7	24	theme	respective	1586:1595	arg1	conjugates					1602:1611	respective MenA conjugates	1586:1611	respective MenA conjugates	1586:1611	Investigating the binding and avidity properties of IgGs induced in mice by non-conjugated carriers, we found that CRM197 induced low levels of anti-carrier antibodies, with decreased avidity for its MenA conjugates and poor binding to DT and respective MenA conjugates.
25448110	9	25	theme	consequent	2012:2021	arg1	alteration					2023:2032	consequent alteration	2012:2032	consequent alteration of the antibody recognition and avidity	2012:2072	The low intrinsic immunogenicity of CRM197 as compared to DT, the structural modifications induced by glycoconjugation and detoxification processes, resulting in conformational changes in CRM197 and DT epitopes with consequent alteration of the antibody recognition and avidity, might explain the different behavior of CRM197 and DT in a carrier priming context.
25448110	4	26	theme	previous	717:724	arg1	observations					726:737	previous observations	717:737	previous observations	717:737	Extending previous observations we show here that priming mice with CRM197 or DT does not suppress the response to the carbohydrate moiety of CRM197 meningococcal serogroup A (MenA) conjugates, while priming with DT can suppress the response to DT-MenA conjugates.
25448110	0	27	theme	different	56:64	arg1	impact					66:71	a different impact	54:71	a different impact	54:71	Carrier priming with CRM 197 or diphtheria toxoid has a different impact on the immunogenicity of the respective glycoconjugates: biophysical and immunochemical interpretation.
25448110	4	28	theme	DT-MenA	952:958	arg1	conjugates					960:969	DT-MenA conjugates	952:969	DT-MenA conjugates	952:969	Extending previous observations we show here that priming mice with CRM197 or DT does not suppress the response to the carbohydrate moiety of CRM197 meningococcal serogroup A (MenA) conjugates, while priming with DT can suppress the response to DT-MenA conjugates.
25448110	9	29	theme	antibody	2041:2048	arg1	recognition					2050:2060	the antibody recognition	2037:2060	the antibody recognition	2037:2060	The low intrinsic immunogenicity of CRM197 as compared to DT, the structural modifications induced by glycoconjugation and detoxification processes, resulting in conformational changes in CRM197 and DT epitopes with consequent alteration of the antibody recognition and avidity, might explain the different behavior of CRM197 and DT in a carrier priming context.
25448110	4	30	theme	priming	757:763	arg1	mice					765:768	priming mice	757:768	priming mice with CRM197 or DT	757:786	Extending previous observations we show here that priming mice with CRM197 or DT does not suppress the response to the carbohydrate moiety of CRM197 meningococcal serogroup A (MenA) conjugates, while priming with DT can suppress the response to DT-MenA conjugates.
25448110	2	31	theme	toxoid	388:393	arg1	mutant					340:345	the non-toxic mutant	326:345	the non-toxic mutant of diphtheria toxin (CRM197), diphtheria toxoid (DT) and tetanus toxoid (TT)	326:422	The main carrier proteins used in commercial conjugate vaccines are the non-toxic mutant of diphtheria toxin (CRM197), diphtheria toxoid (DT) and tetanus toxoid (TT).
25448110	2	31	theme	toxoid	388:393	arg1	proteins					275:282	The main carrier proteins	258:282	The main carrier proteins used in commercial conjugate vaccines	258:320	The main carrier proteins used in commercial conjugate vaccines are the non-toxic mutant of diphtheria toxin (CRM197), diphtheria toxoid (DT) and tetanus toxoid (TT).
25448110	0	32	theme	Carrier	0:6	arg1	priming					8:14	Carrier priming	0:14	Carrier priming with CRM 197 or diphtheria toxoid	0:48	Carrier priming with CRM 197 or diphtheria toxoid has a different impact on the immunogenicity of the respective glycoconjugates: biophysical and immunochemical interpretation.
25448110	4	33	dep	show	742:745	arg1	Extending					707:715	Extending	707:715	Extending previous observations	707:737	Extending previous observations we show here that priming mice with CRM197 or DT does not suppress the response to the carbohydrate moiety of CRM197 meningococcal serogroup A (MenA) conjugates, while priming with DT can suppress the response to DT-MenA conjugates.
25448110	4	34	with	priming	907:913	arg1	DT					920:921	DT	920:921	DT	920:921	Extending previous observations we show here that priming mice with CRM197 or DT does not suppress the response to the carbohydrate moiety of CRM197 meningococcal serogroup A (MenA) conjugates, while priming with DT can suppress the response to DT-MenA conjugates.
25448110	9	35	theme	intrinsic	1804:1812	arg1	immunogenicity					1814:1827	The low intrinsic immunogenicity	1796:1827	The low intrinsic immunogenicity of CRM197	1796:1837	The low intrinsic immunogenicity of CRM197 as compared to DT, the structural modifications induced by glycoconjugation and detoxification processes, resulting in conformational changes in CRM197 and DT epitopes with consequent alteration of the antibody recognition and avidity, might explain the different behavior of CRM197 and DT in a carrier priming context.
25448110	2	36	theme	toxoid	412:417	arg1	mutant					340:345	the non-toxic mutant	326:345	the non-toxic mutant of diphtheria toxin (CRM197), diphtheria toxoid (DT) and tetanus toxoid (TT)	326:422	The main carrier proteins used in commercial conjugate vaccines are the non-toxic mutant of diphtheria toxin (CRM197), diphtheria toxoid (DT) and tetanus toxoid (TT).
25448110	2	36	theme	toxoid	412:417	arg1	proteins					275:282	The main carrier proteins	258:282	The main carrier proteins used in commercial conjugate vaccines	258:320	The main carrier proteins used in commercial conjugate vaccines are the non-toxic mutant of diphtheria toxin (CRM197), diphtheria toxoid (DT) and tetanus toxoid (TT).
25448110	3	37	theme	Modern	425:430	arg1	schedules					462:470	Modern childhood routine vaccination schedules	425:470	Modern childhood routine vaccination schedules	425:470	Modern childhood routine vaccination schedules include the administration of several vaccines simultaneously or in close sequence, increasing the concern that the repeated exposure to conjugates based on these carrier proteins might interfere with the anti-polysaccharide response.
25448110	3	38	theme	carrier	635:641	arg1	proteins					643:650	these carrier proteins	629:650	these carrier proteins	629:650	Modern childhood routine vaccination schedules include the administration of several vaccines simultaneously or in close sequence, increasing the concern that the repeated exposure to conjugates based on these carrier proteins might interfere with the anti-polysaccharide response.
25448110	9	39	theme	different	2093:2101	arg1	behavior					2103:2110	the different behavior	2089:2110	the different behavior of CRM197 and DT in a carrier priming context	2089:2156	The low intrinsic immunogenicity of CRM197 as compared to DT, the structural modifications induced by glycoconjugation and detoxification processes, resulting in conformational changes in CRM197 and DT epitopes with consequent alteration of the antibody recognition and avidity, might explain the different behavior of CRM197 and DT in a carrier priming context.
25448110	2	40	theme	toxin	361:365	arg1	mutant					340:345	the non-toxic mutant	326:345	the non-toxic mutant of diphtheria toxin (CRM197), diphtheria toxoid (DT) and tetanus toxoid (TT)	326:422	The main carrier proteins used in commercial conjugate vaccines are the non-toxic mutant of diphtheria toxin (CRM197), diphtheria toxoid (DT) and tetanus toxoid (TT).
25448110	2	40	theme	toxin	361:365	arg1	proteins					275:282	The main carrier proteins	258:282	The main carrier proteins used in commercial conjugate vaccines	258:320	The main carrier proteins used in commercial conjugate vaccines are the non-toxic mutant of diphtheria toxin (CRM197), diphtheria toxoid (DT) and tetanus toxoid (TT).
25448110	0	41	theme	diphtheria	32:41	arg1	toxoid					43:48	diphtheria toxoid	32:48	diphtheria toxoid	32:48	Carrier priming with CRM 197 or diphtheria toxoid has a different impact on the immunogenicity of the respective glycoconjugates: biophysical and immunochemical interpretation.
25448110	3	42	theme	anti-polysaccharide	677:695	arg1	response					697:704	the anti-polysaccharide response	673:704	the anti-polysaccharide response	673:704	Modern childhood routine vaccination schedules include the administration of several vaccines simultaneously or in close sequence, increasing the concern that the repeated exposure to conjugates based on these carrier proteins might interfere with the anti-polysaccharide response.
25448110	6	43	theme	circular	1128:1135	arg1	dichroism					1137:1145	circular dichroism	1128:1145	circular dichroism	1128:1145	Differential scanning calorimetry and circular dichroism data revealed that the CRM197 structure was altered by the chemical conjugation, while DT and the formaldehyde-treated form of CRM197 were less impacted, depending on the degree of glycosylation.
25448110	3	44	theme	vaccines	510:517	arg1	administration					484:497	the administration	480:497	the administration of several vaccines simultaneously or in close sequence	480:553	Modern childhood routine vaccination schedules include the administration of several vaccines simultaneously or in close sequence, increasing the concern that the repeated exposure to conjugates based on these carrier proteins might interfere with the anti-polysaccharide response.
25448110	7	45	theme	antibodies	1500:1509	arg1	levels					1477:1482	low levels	1473:1482	low levels of anti-carrier antibodies	1473:1509	Investigating the binding and avidity properties of IgGs induced in mice by non-conjugated carriers, we found that CRM197 induced low levels of anti-carrier antibodies, with decreased avidity for its MenA conjugates and poor binding to DT and respective MenA conjugates.
25448110	9	46	theme	structural	1862:1871	arg1	modifications					1873:1885	the structural modifications	1858:1885	the structural modifications induced by glycoconjugation and detoxification processes, resulting in conformational changes in CRM197 and DT epitopes with consequent alteration of the antibody recognition and avidity,	1858:2073	The low intrinsic immunogenicity of CRM197 as compared to DT, the structural modifications induced by glycoconjugation and detoxification processes, resulting in conformational changes in CRM197 and DT epitopes with consequent alteration of the antibody recognition and avidity, might explain the different behavior of CRM197 and DT in a carrier priming context.
25448110	4	47	theme	A	880:880	arg1	conjugates					889:898	CRM197 meningococcal serogroup A (MenA) conjugates	849:898	CRM197 meningococcal serogroup A (MenA) conjugates	849:898	Extending previous observations we show here that priming mice with CRM197 or DT does not suppress the response to the carbohydrate moiety of CRM197 meningococcal serogroup A (MenA) conjugates, while priming with DT can suppress the response to DT-MenA conjugates.
25448110	6	48	theme	Differential	1090:1101	arg1	calorimetry					1112:1122	Differential scanning calorimetry	1090:1122	Differential scanning calorimetry	1090:1122	Differential scanning calorimetry and circular dichroism data revealed that the CRM197 structure was altered by the chemical conjugation, while DT and the formaldehyde-treated form of CRM197 were less impacted, depending on the degree of glycosylation.
25448110	9	49	theme	carrier	2134:2140	arg1	context					2150:2156	a carrier priming context	2132:2156	a carrier priming context	2132:2156	The low intrinsic immunogenicity of CRM197 as compared to DT, the structural modifications induced by glycoconjugation and detoxification processes, resulting in conformational changes in CRM197 and DT epitopes with consequent alteration of the antibody recognition and avidity, might explain the different behavior of CRM197 and DT in a carrier priming context.
25448110	2	50	theme	conjugate	303:311	arg1	vaccines					313:320	commercial conjugate vaccines	292:320	commercial conjugate vaccines	292:320	The main carrier proteins used in commercial conjugate vaccines are the non-toxic mutant of diphtheria toxin (CRM197), diphtheria toxoid (DT) and tetanus toxoid (TT).
25448110	7	51	theme	low	1473:1475	arg1	levels					1477:1482	low levels	1473:1482	low levels of anti-carrier antibodies	1473:1509	Investigating the binding and avidity properties of IgGs induced in mice by non-conjugated carriers, we found that CRM197 induced low levels of anti-carrier antibodies, with decreased avidity for its MenA conjugates and poor binding to DT and respective MenA conjugates.
25448110	8	52	theme	high	1638:1641	arg1	titers					1652:1657	high antibody titers	1638:1657	high antibody titers able to bind with comparable avidity both the protein and its conjugates but showing very low avidity for CRM197 and related conjugates	1638:1793	In contrast, DT induced high antibody titers able to bind with comparable avidity both the protein and its conjugates but showing very low avidity for CRM197 and related conjugates.
25448110	9	53	theme	detoxification	1919:1932	arg1	processes					1934:1942	detoxification processes	1919:1942	detoxification processes	1919:1942	The low intrinsic immunogenicity of CRM197 as compared to DT, the structural modifications induced by glycoconjugation and detoxification processes, resulting in conformational changes in CRM197 and DT epitopes with consequent alteration of the antibody recognition and avidity, might explain the different behavior of CRM197 and DT in a carrier priming context.
25448110	2	54	theme	non-toxic	330:338	arg1	mutant					340:345	the non-toxic mutant	326:345	the non-toxic mutant of diphtheria toxin (CRM197), diphtheria toxoid (DT) and tetanus toxoid (TT)	326:422	The main carrier proteins used in commercial conjugate vaccines are the non-toxic mutant of diphtheria toxin (CRM197), diphtheria toxoid (DT) and tetanus toxoid (TT).
25448110	2	54	theme	non-toxic	330:338	arg1	proteins					275:282	The main carrier proteins	258:282	The main carrier proteins used in commercial conjugate vaccines	258:320	The main carrier proteins used in commercial conjugate vaccines are the non-toxic mutant of diphtheria toxin (CRM197), diphtheria toxoid (DT) and tetanus toxoid (TT).
25448110	4	55	theme	meningococcal	856:868	arg1	MenA					883:886	MenA	883:886	MenA	883:886	Extending previous observations we show here that priming mice with CRM197 or DT does not suppress the response to the carbohydrate moiety of CRM197 meningococcal serogroup A (MenA) conjugates, while priming with DT can suppress the response to DT-MenA conjugates.
25448110	4	55	theme	meningococcal	856:868	arg1	A					880:880	CRM197 meningococcal serogroup A	849:880	CRM197 meningococcal serogroup A (MenA) conjugates	849:898	Extending previous observations we show here that priming mice with CRM197 or DT does not suppress the response to the carbohydrate moiety of CRM197 meningococcal serogroup A (MenA) conjugates, while priming with DT can suppress the response to DT-MenA conjugates.
25448110	6	56	theme	CRM197	1170:1175	arg1	structure					1177:1185	the CRM197 structure	1166:1185	the CRM197 structure	1166:1185	Differential scanning calorimetry and circular dichroism data revealed that the CRM197 structure was altered by the chemical conjugation, while DT and the formaldehyde-treated form of CRM197 were less impacted, depending on the degree of glycosylation.
25448110	0	57	theme	respective	102:111	arg1	glycoconjugates					113:127	the respective glycoconjugates	98:127	the respective glycoconjugates: biophysical and immunochemical interpretation	98:174	Carrier priming with CRM 197 or diphtheria toxoid has a different impact on the immunogenicity of the respective glycoconjugates: biophysical and immunochemical interpretation.
25448110	4	58	theme	carbohydrate	826:837	arg1	moiety					839:844	the carbohydrate moiety	822:844	the carbohydrate moiety of CRM197 meningococcal serogroup A (MenA) conjugates	822:898	Extending previous observations we show here that priming mice with CRM197 or DT does not suppress the response to the carbohydrate moiety of CRM197 meningococcal serogroup A (MenA) conjugates, while priming with DT can suppress the response to DT-MenA conjugates.
25448110	7	59	dep	binding	1361:1367	arg1	properties					1381:1390	properties	1381:1390	properties	1381:1390	Investigating the binding and avidity properties of IgGs induced in mice by non-conjugated carriers, we found that CRM197 induced low levels of anti-carrier antibodies, with decreased avidity for its MenA conjugates and poor binding to DT and respective MenA conjugates.
25448110	7	59	dep	binding	1361:1367	arg1	the					1357:1359	the	1357:1359	the	1357:1359	Investigating the binding and avidity properties of IgGs induced in mice by non-conjugated carriers, we found that CRM197 induced low levels of anti-carrier antibodies, with decreased avidity for its MenA conjugates and poor binding to DT and respective MenA conjugates.
25448110	5	60	dep	made	1001:1004	arg1	explain					975:981	explain	975:981	To explain these findings	972:996	To explain these findings we made use of biophysical and immunochemical techniques applied mainly to MenA conjugates.
25448110	5	61	theme	MenA	1073:1076	arg1	conjugates					1078:1087	MenA conjugates	1073:1087	MenA conjugates	1073:1087	To explain these findings we made use of biophysical and immunochemical techniques applied mainly to MenA conjugates.
25448110	3	62	theme	repeated	588:595	arg1	exposure					597:604	the repeated exposure	584:604	the repeated exposure to conjugates based on these carrier proteins	584:650	Modern childhood routine vaccination schedules include the administration of several vaccines simultaneously or in close sequence, increasing the concern that the repeated exposure to conjugates based on these carrier proteins might interfere with the anti-polysaccharide response.
25448110	4	63	with	mice	765:768	arg1	DT					785:786	DT	785:786	DT	785:786	Extending previous observations we show here that priming mice with CRM197 or DT does not suppress the response to the carbohydrate moiety of CRM197 meningococcal serogroup A (MenA) conjugates, while priming with DT can suppress the response to DT-MenA conjugates.
25448110	4	63	with	mice	765:768	arg1	CRM197					775:780	CRM197	775:780	CRM197	775:780	Extending previous observations we show here that priming mice with CRM197 or DT does not suppress the response to the carbohydrate moiety of CRM197 meningococcal serogroup A (MenA) conjugates, while priming with DT can suppress the response to DT-MenA conjugates.
25448110	8	64	theme	low	1749:1751	arg1	avidity					1753:1759	very low avidity	1744:1759	very low avidity for CRM197 and related conjugates	1744:1793	In contrast, DT induced high antibody titers able to bind with comparable avidity both the protein and its conjugates but showing very low avidity for CRM197 and related conjugates.
25448110	4	65	theme	conjugates	889:898	arg1	moiety					839:844	the carbohydrate moiety	822:844	the carbohydrate moiety of CRM197 meningococcal serogroup A (MenA) conjugates	822:898	Extending previous observations we show here that priming mice with CRM197 or DT does not suppress the response to the carbohydrate moiety of CRM197 meningococcal serogroup A (MenA) conjugates, while priming with DT can suppress the response to DT-MenA conjugates.
25448110	7	66	theme	IgGs	1395:1398	arg1	avidity					1373:1379	avidity	1373:1379	avidity	1373:1379	Investigating the binding and avidity properties of IgGs induced in mice by non-conjugated carriers, we found that CRM197 induced low levels of anti-carrier antibodies, with decreased avidity for its MenA conjugates and poor binding to DT and respective MenA conjugates.
25448110	7	66	theme	IgGs	1395:1398	arg1	binding					1361:1367	binding	1361:1367	binding	1361:1367	Investigating the binding and avidity properties of IgGs induced in mice by non-conjugated carriers, we found that CRM197 induced low levels of anti-carrier antibodies, with decreased avidity for its MenA conjugates and poor binding to DT and respective MenA conjugates.
25448110	9	67	theme	CRM197	1984:1989	arg1	epitopes					1998:2005	CRM197 and DT epitopes	1984:2005	CRM197 and DT epitopes with consequent alteration of the antibody recognition and avidity	1984:2072	The low intrinsic immunogenicity of CRM197 as compared to DT, the structural modifications induced by glycoconjugation and detoxification processes, resulting in conformational changes in CRM197 and DT epitopes with consequent alteration of the antibody recognition and avidity, might explain the different behavior of CRM197 and DT in a carrier priming context.
25448110	5	68	theme	techniques	1044:1053	arg1	use					1006:1008	use	1006:1008	use of biophysical and immunochemical techniques applied mainly to MenA conjugates	1006:1087	To explain these findings we made use of biophysical and immunochemical techniques applied mainly to MenA conjugates.
25448110	7	69	theme	MenA	1597:1600	arg1	conjugates					1602:1611	respective MenA conjugates	1586:1611	respective MenA conjugates	1586:1611	Investigating the binding and avidity properties of IgGs induced in mice by non-conjugated carriers, we found that CRM197 induced low levels of anti-carrier antibodies, with decreased avidity for its MenA conjugates and poor binding to DT and respective MenA conjugates.
25448110	9	70	theme	DT	1995:1996	arg1	epitopes					1998:2005	CRM197 and DT epitopes	1984:2005	CRM197 and DT epitopes with consequent alteration of the antibody recognition and avidity	1984:2072	The low intrinsic immunogenicity of CRM197 as compared to DT, the structural modifications induced by glycoconjugation and detoxification processes, resulting in conformational changes in CRM197 and DT epitopes with consequent alteration of the antibody recognition and avidity, might explain the different behavior of CRM197 and DT in a carrier priming context.
25448110	2	71	theme	main	262:265	arg1	mutant					340:345	the non-toxic mutant	326:345	the non-toxic mutant of diphtheria toxin (CRM197), diphtheria toxoid (DT) and tetanus toxoid (TT)	326:422	The main carrier proteins used in commercial conjugate vaccines are the non-toxic mutant of diphtheria toxin (CRM197), diphtheria toxoid (DT) and tetanus toxoid (TT).
25448110	2	71	theme	main	262:265	arg1	proteins					275:282	The main carrier proteins	258:282	The main carrier proteins used in commercial conjugate vaccines	258:320	The main carrier proteins used in commercial conjugate vaccines are the non-toxic mutant of diphtheria toxin (CRM197), diphtheria toxoid (DT) and tetanus toxoid (TT).
25448110	6	72	theme	chemical	1206:1213	arg1	conjugation					1215:1225	the chemical conjugation	1202:1225	the chemical conjugation	1202:1225	Differential scanning calorimetry and circular dichroism data revealed that the CRM197 structure was altered by the chemical conjugation, while DT and the formaldehyde-treated form of CRM197 were less impacted, depending on the degree of glycosylation.
25448110	8	73	dep	able	1659:1662	arg1	bind					1667:1670	bind	1667:1670	to bind with comparable avidity both the protein and its conjugates	1664:1730	In contrast, DT induced high antibody titers able to bind with comparable avidity both the protein and its conjugates but showing very low avidity for CRM197 and related conjugates.
25448110	8	73	dep	able	1659:1662	arg1	showing					1736:1742	showing	1736:1742	showing very low avidity for CRM197 and related conjugates	1736:1793	In contrast, DT induced high antibody titers able to bind with comparable avidity both the protein and its conjugates but showing very low avidity for CRM197 and related conjugates.
25448110	9	74	with	epitopes	1998:2005	arg1	alteration					2023:2032	consequent alteration	2012:2032	consequent alteration of the antibody recognition and avidity	2012:2072	The low intrinsic immunogenicity of CRM197 as compared to DT, the structural modifications induced by glycoconjugation and detoxification processes, resulting in conformational changes in CRM197 and DT epitopes with consequent alteration of the antibody recognition and avidity, might explain the different behavior of CRM197 and DT in a carrier priming context.
25448110	9	75	from	behavior	2103:2110	arg1	context					2150:2156	a carrier priming context	2132:2156	a carrier priming context	2132:2156	The low intrinsic immunogenicity of CRM197 as compared to DT, the structural modifications induced by glycoconjugation and detoxification processes, resulting in conformational changes in CRM197 and DT epitopes with consequent alteration of the antibody recognition and avidity, might explain the different behavior of CRM197 and DT in a carrier priming context.
25448110	7	76	theme	poor	1563:1566	arg1	binding					1568:1574	poor binding	1563:1574	poor binding to DT and respective MenA conjugates	1563:1611	Investigating the binding and avidity properties of IgGs induced in mice by non-conjugated carriers, we found that CRM197 induced low levels of anti-carrier antibodies, with decreased avidity for its MenA conjugates and poor binding to DT and respective MenA conjugates.
25448110	9	77	theme	recognition	2050:2060	arg1	alteration					2023:2032	consequent alteration	2012:2032	consequent alteration of the antibody recognition and avidity	2012:2072	The low intrinsic immunogenicity of CRM197 as compared to DT, the structural modifications induced by glycoconjugation and detoxification processes, resulting in conformational changes in CRM197 and DT epitopes with consequent alteration of the antibody recognition and avidity, might explain the different behavior of CRM197 and DT in a carrier priming context.
25448110	9	78	theme	low	1800:1802	arg1	immunogenicity					1814:1827	The low intrinsic immunogenicity	1796:1827	The low intrinsic immunogenicity of CRM197	1796:1837	The low intrinsic immunogenicity of CRM197 as compared to DT, the structural modifications induced by glycoconjugation and detoxification processes, resulting in conformational changes in CRM197 and DT epitopes with consequent alteration of the antibody recognition and avidity, might explain the different behavior of CRM197 and DT in a carrier priming context.
25448110	8	79	dep	protein	1705:1711	arg1	the					1701:1703	the	1701:1703	the	1701:1703	In contrast, DT induced high antibody titers able to bind with comparable avidity both the protein and its conjugates but showing very low avidity for CRM197 and related conjugates.
25448110	2	80	theme	diphtheria	377:386	arg1	DT					396:397	DT	396:397	DT	396:397	The main carrier proteins used in commercial conjugate vaccines are the non-toxic mutant of diphtheria toxin (CRM197), diphtheria toxoid (DT) and tetanus toxoid (TT).
25448110	2	80	theme	diphtheria	377:386	arg1	toxoid					388:393	diphtheria toxoid	377:393	diphtheria toxoid (DT)	377:398	The main carrier proteins used in commercial conjugate vaccines are the non-toxic mutant of diphtheria toxin (CRM197), diphtheria toxoid (DT) and tetanus toxoid (TT).
25448110	2	81	theme	tetanus	404:410	arg1	TT					420:421	TT	420:421	TT	420:421	The main carrier proteins used in commercial conjugate vaccines are the non-toxic mutant of diphtheria toxin (CRM197), diphtheria toxoid (DT) and tetanus toxoid (TT).
25448110	2	81	theme	tetanus	404:410	arg1	toxoid					412:417	tetanus toxoid	404:417	tetanus toxoid (TT)	404:422	The main carrier proteins used in commercial conjugate vaccines are the non-toxic mutant of diphtheria toxin (CRM197), diphtheria toxoid (DT) and tetanus toxoid (TT).
25448110	9	82	from	changes	1973:1979	arg1	epitopes					1998:2005	CRM197 and DT epitopes	1984:2005	CRM197 and DT epitopes with consequent alteration of the antibody recognition and avidity	1984:2072	The low intrinsic immunogenicity of CRM197 as compared to DT, the structural modifications induced by glycoconjugation and detoxification processes, resulting in conformational changes in CRM197 and DT epitopes with consequent alteration of the antibody recognition and avidity, might explain the different behavior of CRM197 and DT in a carrier priming context.
25448110	9	83	theme	CRM197	1832:1837	arg1	immunogenicity					1814:1827	The low intrinsic immunogenicity	1796:1827	The low intrinsic immunogenicity of CRM197	1796:1837	The low intrinsic immunogenicity of CRM197 as compared to DT, the structural modifications induced by glycoconjugation and detoxification processes, resulting in conformational changes in CRM197 and DT epitopes with consequent alteration of the antibody recognition and avidity, might explain the different behavior of CRM197 and DT in a carrier priming context.
25448110	3	84	theme	childhood	432:440	arg1	schedules					462:470	Modern childhood routine vaccination schedules	425:470	Modern childhood routine vaccination schedules	425:470	Modern childhood routine vaccination schedules include the administration of several vaccines simultaneously or in close sequence, increasing the concern that the repeated exposure to conjugates based on these carrier proteins might interfere with the anti-polysaccharide response.
25448110	7	85	theme	MenA	1543:1546	arg1	conjugates					1548:1557	its MenA conjugates	1539:1557	its MenA conjugates	1539:1557	Investigating the binding and avidity properties of IgGs induced in mice by non-conjugated carriers, we found that CRM197 induced low levels of anti-carrier antibodies, with decreased avidity for its MenA conjugates and poor binding to DT and respective MenA conjugates.
25448110	5	86	theme	biophysical	1013:1023	arg1	techniques					1044:1053	biophysical and immunochemical techniques	1013:1053	biophysical and immunochemical techniques applied mainly to MenA conjugates	1013:1087	To explain these findings we made use of biophysical and immunochemical techniques applied mainly to MenA conjugates.
25448110	3	87	theme	vaccination	450:460	arg1	schedules					462:470	Modern childhood routine vaccination schedules	425:470	Modern childhood routine vaccination schedules	425:470	Modern childhood routine vaccination schedules include the administration of several vaccines simultaneously or in close sequence, increasing the concern that the repeated exposure to conjugates based on these carrier proteins might interfere with the anti-polysaccharide response.
25448110	6	88	theme	glycosylation	1328:1340	arg1	degree					1318:1323	the degree	1314:1323	the degree of glycosylation	1314:1340	Differential scanning calorimetry and circular dichroism data revealed that the CRM197 structure was altered by the chemical conjugation, while DT and the formaldehyde-treated form of CRM197 were less impacted, depending on the degree of glycosylation.
25448110	6	89	dep	calorimetry	1112:1122	arg1	data					1147:1150	data	1147:1150	data	1147:1150	Differential scanning calorimetry and circular dichroism data revealed that the CRM197 structure was altered by the chemical conjugation, while DT and the formaldehyde-treated form of CRM197 were less impacted, depending on the degree of glycosylation.
25448110	0	90	with	priming	8:14	arg1	CRM					21:23	CRM 197	21:27	CRM 197	21:27	Carrier priming with CRM 197 or diphtheria toxoid has a different impact on the immunogenicity of the respective glycoconjugates: biophysical and immunochemical interpretation.
25448110	0	90	with	priming	8:14	arg1	toxoid					43:48	diphtheria toxoid	32:48	diphtheria toxoid	32:48	Carrier priming with CRM 197 or diphtheria toxoid has a different impact on the immunogenicity of the respective glycoconjugates: biophysical and immunochemical interpretation.
25448110	2	91	theme	diphtheria	350:359	arg1	CRM197					368:373	CRM197	368:373	CRM197	368:373	The main carrier proteins used in commercial conjugate vaccines are the non-toxic mutant of diphtheria toxin (CRM197), diphtheria toxoid (DT) and tetanus toxoid (TT).
25448110	2	91	theme	diphtheria	350:359	arg1	toxin					361:365	diphtheria toxin	350:365	diphtheria toxin (CRM197)	350:374	The main carrier proteins used in commercial conjugate vaccines are the non-toxic mutant of diphtheria toxin (CRM197), diphtheria toxoid (DT) and tetanus toxoid (TT).
25448110	8	92	theme	able	1659:1662	arg1	titers					1652:1657	high antibody titers	1638:1657	high antibody titers able to bind with comparable avidity both the protein and its conjugates but showing very low avidity for CRM197 and related conjugates	1638:1793	In contrast, DT induced high antibody titers able to bind with comparable avidity both the protein and its conjugates but showing very low avidity for CRM197 and related conjugates.
25448110	9	93	theme	CRM197	2115:2120	arg1	behavior					2103:2110	the different behavior	2089:2110	the different behavior of CRM197 and DT in a carrier priming context	2089:2156	The low intrinsic immunogenicity of CRM197 as compared to DT, the structural modifications induced by glycoconjugation and detoxification processes, resulting in conformational changes in CRM197 and DT epitopes with consequent alteration of the antibody recognition and avidity, might explain the different behavior of CRM197 and DT in a carrier priming context.
25448110	1	94	theme	infectious	237:246	arg1	diseases					248:255	infectious diseases	237:255	infectious diseases	237:255	Glycoconjugate vaccines play an enormous role in preventing infectious diseases.
25448110	7	95	theme	decreased	1517:1525	arg1	avidity					1527:1533	decreased avidity	1517:1533	decreased avidity for its MenA conjugates and poor binding to DT and respective MenA conjugates	1517:1611	Investigating the binding and avidity properties of IgGs induced in mice by non-conjugated carriers, we found that CRM197 induced low levels of anti-carrier antibodies, with decreased avidity for its MenA conjugates and poor binding to DT and respective MenA conjugates.
25448110	9	96	theme	DT	2126:2127	arg1	behavior					2103:2110	the different behavior	2089:2110	the different behavior of CRM197 and DT in a carrier priming context	2089:2156	The low intrinsic immunogenicity of CRM197 as compared to DT, the structural modifications induced by glycoconjugation and detoxification processes, resulting in conformational changes in CRM197 and DT epitopes with consequent alteration of the antibody recognition and avidity, might explain the different behavior of CRM197 and DT in a carrier priming context.
25448110	0	97	contain	has	50:52	arg2	impact					66:71	a different impact	54:71	a different impact	54:71	Carrier priming with CRM 197 or diphtheria toxoid has a different impact on the immunogenicity of the respective glycoconjugates: biophysical and immunochemical interpretation.
25448110	0	97	contain	has	50:52	arg1	priming					8:14	Carrier priming	0:14	Carrier priming with CRM 197 or diphtheria toxoid	0:48	Carrier priming with CRM 197 or diphtheria toxoid has a different impact on the immunogenicity of the respective glycoconjugates: biophysical and immunochemical interpretation.
25448110	8	98	theme	comparable	1677:1686	arg1	avidity					1688:1694	comparable avidity	1677:1694	comparable avidity	1677:1694	In contrast, DT induced high antibody titers able to bind with comparable avidity both the protein and its conjugates but showing very low avidity for CRM197 and related conjugates.
26056988	6	0	from	studies	910:916	arg1	conditions					935:944	the simulated conditions	921:944	the simulated conditions	921:944	The release studies in the simulated conditions reveal that these hydrogels can be used as delivery vehicles for oral administration of DX targeting the intestine.
26056988	4	1	theme	containing	764:773	arg1	hydrogels					775:783	the CD containing hydrogels	757:783	the CD containing hydrogels	757:783	Moreover, the drug release rate is found to show a strong dependence on the GG content and the effect of GG is more pronounced in case of the CD containing hydrogels.
26056988	6	2	theme	delivery	989:996	arg1	hydrogels					964:972	these hydrogels	958:972	these hydrogels	958:972	The release studies in the simulated conditions reveal that these hydrogels can be used as delivery vehicles for oral administration of DX targeting the intestine.
26056988	6	2	theme	delivery	989:996	arg1	vehicles					998:1005	delivery vehicles	989:1005	delivery vehicles for oral administration of DX targeting the intestine	989:1059	The release studies in the simulated conditions reveal that these hydrogels can be used as delivery vehicles for oral administration of DX targeting the intestine.
26056988	4	3	theme	release	638:644	arg1	rate					646:649	the drug release rate	629:649	the drug release rate	629:649	Moreover, the drug release rate is found to show a strong dependence on the GG content and the effect of GG is more pronounced in case of the CD containing hydrogels.
26056988	4	4	theme	GG	695:696	arg1	content					698:704	the GG content	691:704	the GG content	691:704	Moreover, the drug release rate is found to show a strong dependence on the GG content and the effect of GG is more pronounced in case of the CD containing hydrogels.
26056988	6	5	theme	DX	1034:1035	arg1	administration					1016:1029	oral administration	1011:1029	oral administration of DX targeting the intestine	1011:1059	The release studies in the simulated conditions reveal that these hydrogels can be used as delivery vehicles for oral administration of DX targeting the intestine.
26056988	5	6	theme	drug	827:830	arg1	release					832:838	drug release	827:838	drug release	827:838	As the GG content increases, the rate of drug release decreases considerably and the drug release is prolonged.
26056988	1	7	theme	non-toxic	204:212	arg1	crosslinker					214:224	a non-toxic crosslinker	202:224	a non-toxic crosslinker	202:224	pH-Responsive hydrogel systems based on guar gum (GG), poly(acrylic acid) (PAA) and β-cyclodextrin (CD) using a non-toxic crosslinker, tetraethyl orthosilicate for intestinal delivery of dexamethasone (DX) has been reported.
26056988	7	8	theme	delivery	1170:1177	arg1	them					1152:1155	them	1152:1155	them	1152:1155	Cell viability studies reveal the hydrogels to be biocompatible in nature thus validating them as good drug delivery systems.
26056988	7	8	theme	delivery	1170:1177	arg1	systems					1179:1185	good drug delivery systems	1160:1185	good drug delivery systems	1160:1185	Cell viability studies reveal the hydrogels to be biocompatible in nature thus validating them as good drug delivery systems.
26056988	5	9	theme	drug	871:874	arg1	prolonged					887:895	prolonged	887:895	prolonged	887:895	As the GG content increases, the rate of drug release decreases considerably and the drug release is prolonged.
26056988	5	9	theme	drug	871:874	arg1	release					876:882	the drug release	867:882	the drug release	867:882	As the GG content increases, the rate of drug release decreases considerably and the drug release is prolonged.
26056988	2	10	theme	compositions	340:351	arg1	Hydrogels					317:325	Hydrogels	317:325	Hydrogels of different compositions	317:351	Hydrogels of different compositions were synthesized and evaluated for the controlled delivery of DX.
26056988	0	11	theme	pH-Responsive	0:12	arg1	gum					19:21	pH-Responsive guar gum	0:21	pH-Responsive guar gum	0:21	pH-Responsive guar gum hydrogels for controlled delivery of dexamethasone to the intestine.
26056988	2	12	theme	different	330:338	arg1	compositions					340:351	different compositions	330:351	different compositions	330:351	Hydrogels of different compositions were synthesized and evaluated for the controlled delivery of DX.
26056988	0	13	theme	guar	14:17	arg1	gum					19:21	pH-Responsive guar gum	0:21	pH-Responsive guar gum	0:21	pH-Responsive guar gum hydrogels for controlled delivery of dexamethasone to the intestine.
26056988	4	14	theme	CD	761:762	arg1	hydrogels					775:783	the CD containing hydrogels	757:783	the CD containing hydrogels	757:783	Moreover, the drug release rate is found to show a strong dependence on the GG content and the effect of GG is more pronounced in case of the CD containing hydrogels.
26056988	4	15	from	dependence	677:686	arg1	content					698:704	the GG content	691:704	the GG content	691:704	Moreover, the drug release rate is found to show a strong dependence on the GG content and the effect of GG is more pronounced in case of the CD containing hydrogels.
26056988	4	16	theme	strong	670:675	arg1	dependence					677:686	a strong dependence	668:686	a strong dependence on the GG content	668:704	Moreover, the drug release rate is found to show a strong dependence on the GG content and the effect of GG is more pronounced in case of the CD containing hydrogels.
26056988	7	17	theme	drug	1165:1168	arg1	them					1152:1155	them	1152:1155	them	1152:1155	Cell viability studies reveal the hydrogels to be biocompatible in nature thus validating them as good drug delivery systems.
26056988	7	17	theme	drug	1165:1168	arg1	systems					1179:1185	good drug delivery systems	1160:1185	good drug delivery systems	1160:1185	Cell viability studies reveal the hydrogels to be biocompatible in nature thus validating them as good drug delivery systems.
26056988	1	18	theme	acrylic	152:158	arg1	poly					147:150	poly	147:150	poly(acrylic acid) (PAA)	147:170	pH-Responsive hydrogel systems based on guar gum (GG), poly(acrylic acid) (PAA) and β-cyclodextrin (CD) using a non-toxic crosslinker, tetraethyl orthosilicate for intestinal delivery of dexamethasone (DX) has been reported.
26056988	1	18	theme	acrylic	152:158	arg1	acid					160:163	acrylic acid	152:163	acrylic acid	152:163	pH-Responsive hydrogel systems based on guar gum (GG), poly(acrylic acid) (PAA) and β-cyclodextrin (CD) using a non-toxic crosslinker, tetraethyl orthosilicate for intestinal delivery of dexamethasone (DX) has been reported.
26056988	2	19	theme	controlled	392:401	arg1	delivery					403:410	the controlled delivery	388:410	the controlled delivery of DX	388:416	Hydrogels of different compositions were synthesized and evaluated for the controlled delivery of DX.
26056988	7	20	from	nature	1129:1134	arg1	biocompatible					1112:1124	biocompatible	1112:1124	biocompatible	1112:1124	Cell viability studies reveal the hydrogels to be biocompatible in nature thus validating them as good drug delivery systems.
26056988	1	21	theme	pH-Responsive	92:104	arg1	systems					115:121	pH-Responsive hydrogel systems	92:121	pH-Responsive hydrogel systems based on guar gum (GG), poly(acrylic acid) (PAA) and β-cyclodextrin (CD)	92:194	pH-Responsive hydrogel systems based on guar gum (GG), poly(acrylic acid) (PAA) and β-cyclodextrin (CD) using a non-toxic crosslinker, tetraethyl orthosilicate for intestinal delivery of dexamethasone (DX) has been reported.
26056988	4	22	theme	GG	724:725	arg1	pronounced					735:744	pronounced	735:744	pronounced	735:744	Moreover, the drug release rate is found to show a strong dependence on the GG content and the effect of GG is more pronounced in case of the CD containing hydrogels.
26056988	4	22	theme	GG	724:725	arg1	effect					714:719	the effect	710:719	the effect of GG	710:725	Moreover, the drug release rate is found to show a strong dependence on the GG content and the effect of GG is more pronounced in case of the CD containing hydrogels.
26056988	1	23	theme	hydrogel	106:113	arg1	systems					115:121	pH-Responsive hydrogel systems	92:121	pH-Responsive hydrogel systems based on guar gum (GG), poly(acrylic acid) (PAA) and β-cyclodextrin (CD)	92:194	pH-Responsive hydrogel systems based on guar gum (GG), poly(acrylic acid) (PAA) and β-cyclodextrin (CD) using a non-toxic crosslinker, tetraethyl orthosilicate for intestinal delivery of dexamethasone (DX) has been reported.
26056988	5	24	theme	release	832:838	arg1	rate					819:822	the rate	815:822	the rate of drug release	815:838	As the GG content increases, the rate of drug release decreases considerably and the drug release is prolonged.
26056988	1	25	theme	tetraethyl	227:236	arg1	orthosilicate					238:250	tetraethyl orthosilicate	227:250	tetraethyl orthosilicate for intestinal delivery of dexamethasone (DX)	227:296	pH-Responsive hydrogel systems based on guar gum (GG), poly(acrylic acid) (PAA) and β-cyclodextrin (CD) using a non-toxic crosslinker, tetraethyl orthosilicate for intestinal delivery of dexamethasone (DX) has been reported.
26056988	3	26	theme	CD-containing	522:534	arg1	hydrogels					536:544	The CD-containing hydrogels	518:544	The CD-containing hydrogels	518:544	The hydrogels exhibited pH-responsive swelling behavior with a maximum swelling around neutral pH. The CD-containing hydrogels deliver the drug at much slower rate in contrast to the ones without CD.
26056988	0	27	theme	controlled	37:46	arg1	delivery					48:55	controlled delivery	37:55	controlled delivery of dexamethasone to the intestine	37:89	pH-Responsive guar gum hydrogels for controlled delivery of dexamethasone to the intestine.
26056988	6	28	used	used	981:984	arg2	hydrogels					964:972	these hydrogels	958:972	these hydrogels	958:972	The release studies in the simulated conditions reveal that these hydrogels can be used as delivery vehicles for oral administration of DX targeting the intestine.
26056988	6	28	used	used	981:984	arg2	vehicles					998:1005	delivery vehicles	989:1005	delivery vehicles for oral administration of DX targeting the intestine	989:1059	The release studies in the simulated conditions reveal that these hydrogels can be used as delivery vehicles for oral administration of DX targeting the intestine.
26056988	6	29	theme	simulated	925:933	arg1	conditions					935:944	the simulated conditions	921:944	the simulated conditions	921:944	The release studies in the simulated conditions reveal that these hydrogels can be used as delivery vehicles for oral administration of DX targeting the intestine.
26056988	2	30	theme	DX	415:416	arg1	delivery					403:410	the controlled delivery	388:410	the controlled delivery of DX	388:416	Hydrogels of different compositions were synthesized and evaluated for the controlled delivery of DX.
26056988	1	31	theme	intestinal	256:265	arg1	delivery					267:274	intestinal delivery	256:274	intestinal delivery of dexamethasone (DX)	256:296	pH-Responsive hydrogel systems based on guar gum (GG), poly(acrylic acid) (PAA) and β-cyclodextrin (CD) using a non-toxic crosslinker, tetraethyl orthosilicate for intestinal delivery of dexamethasone (DX) has been reported.
26056988	3	32	theme	pH-responsive	443:455	arg1	behavior					466:473	pH-responsive swelling behavior	443:473	pH-responsive swelling behavior	443:473	The hydrogels exhibited pH-responsive swelling behavior with a maximum swelling around neutral pH. The CD-containing hydrogels deliver the drug at much slower rate in contrast to the ones without CD.
26056988	3	33	theme	swelling	457:464	arg1	behavior					466:473	pH-responsive swelling behavior	443:473	pH-responsive swelling behavior	443:473	The hydrogels exhibited pH-responsive swelling behavior with a maximum swelling around neutral pH. The CD-containing hydrogels deliver the drug at much slower rate in contrast to the ones without CD.
26056988	7	34	from	biocompatible	1112:1124	arg1	nature					1129:1134	nature	1129:1134	nature	1129:1134	Cell viability studies reveal the hydrogels to be biocompatible in nature thus validating them as good drug delivery systems.
26056988	1	35	theme	guar	132:135	arg1	GG					142:143	GG	142:143	GG	142:143	pH-Responsive hydrogel systems based on guar gum (GG), poly(acrylic acid) (PAA) and β-cyclodextrin (CD) using a non-toxic crosslinker, tetraethyl orthosilicate for intestinal delivery of dexamethasone (DX) has been reported.
26056988	1	35	theme	guar	132:135	arg1	gum					137:139	guar gum	132:139	guar gum (GG)	132:144	pH-Responsive hydrogel systems based on guar gum (GG), poly(acrylic acid) (PAA) and β-cyclodextrin (CD) using a non-toxic crosslinker, tetraethyl orthosilicate for intestinal delivery of dexamethasone (DX) has been reported.
26056988	7	36	theme	viability	1067:1075	arg1	studies					1077:1083	Cell viability studies	1062:1083	Cell viability studies	1062:1083	Cell viability studies reveal the hydrogels to be biocompatible in nature thus validating them as good drug delivery systems.
26056988	3	37	dep	pH.	514:516	arg1	deliver					546:552	deliver	546:552	deliver the drug at much slower rate in contrast to the ones without CD	546:616	The hydrogels exhibited pH-responsive swelling behavior with a maximum swelling around neutral pH. The CD-containing hydrogels deliver the drug at much slower rate in contrast to the ones without CD.
26056988	6	38	theme	oral	1011:1014	arg1	administration					1016:1029	oral administration	1011:1029	oral administration of DX targeting the intestine	1011:1059	The release studies in the simulated conditions reveal that these hydrogels can be used as delivery vehicles for oral administration of DX targeting the intestine.
26056988	5	39	theme	GG	793:794	arg1	content					796:802	the GG content	789:802	the GG content	789:802	As the GG content increases, the rate of drug release decreases considerably and the drug release is prolonged.
26056988	3	40	theme	slower	571:576	arg1	rate					578:581	much slower rate	566:581	much slower rate	566:581	The hydrogels exhibited pH-responsive swelling behavior with a maximum swelling around neutral pH. The CD-containing hydrogels deliver the drug at much slower rate in contrast to the ones without CD.
26056988	4	41	theme	drug	633:636	arg1	rate					646:649	the drug release rate	629:649	the drug release rate	629:649	Moreover, the drug release rate is found to show a strong dependence on the GG content and the effect of GG is more pronounced in case of the CD containing hydrogels.
26056988	3	42	dep	ones	602:605	arg1	contrast					586:593	contrast	586:593	contrast	586:593	The hydrogels exhibited pH-responsive swelling behavior with a maximum swelling around neutral pH. The CD-containing hydrogels deliver the drug at much slower rate in contrast to the ones without CD.
26056988	0	43	theme	dexamethasone	60:72	arg1	delivery					48:55	controlled delivery	37:55	controlled delivery of dexamethasone to the intestine	37:89	pH-Responsive guar gum hydrogels for controlled delivery of dexamethasone to the intestine.
26056988	1	44	theme	dexamethasone	279:291	arg1	delivery					267:274	intestinal delivery	256:274	intestinal delivery of dexamethasone (DX)	256:296	pH-Responsive hydrogel systems based on guar gum (GG), poly(acrylic acid) (PAA) and β-cyclodextrin (CD) using a non-toxic crosslinker, tetraethyl orthosilicate for intestinal delivery of dexamethasone (DX) has been reported.
26056988	7	45	theme	Cell	1062:1065	arg1	studies					1077:1083	Cell viability studies	1062:1083	Cell viability studies	1062:1083	Cell viability studies reveal the hydrogels to be biocompatible in nature thus validating them as good drug delivery systems.
26056988	7	46	theme	good	1160:1163	arg1	them					1152:1155	them	1152:1155	them	1152:1155	Cell viability studies reveal the hydrogels to be biocompatible in nature thus validating them as good drug delivery systems.
26056988	7	46	theme	good	1160:1163	arg1	systems					1179:1185	good drug delivery systems	1160:1185	good drug delivery systems	1160:1185	Cell viability studies reveal the hydrogels to be biocompatible in nature thus validating them as good drug delivery systems.
26056988	3	47	theme	maximum	482:488	arg1	swelling					490:497	a maximum swelling	480:497	a maximum swelling around neutral pH. The CD-containing hydrogels deliver the drug at much slower rate in contrast to the ones without CD	480:616	The hydrogels exhibited pH-responsive swelling behavior with a maximum swelling around neutral pH. The CD-containing hydrogels deliver the drug at much slower rate in contrast to the ones without CD.
26056988	6	48	theme	release	902:908	arg1	studies					910:916	The release studies	898:916	The release studies in the simulated conditions	898:944	The release studies in the simulated conditions reveal that these hydrogels can be used as delivery vehicles for oral administration of DX targeting the intestine.
24596138	6	0	theme	improved	847:854	arg1	understanding					856:868	an improved understanding	844:868	an improved understanding of those mechanisms	844:888	Here, we describe the hierarchical levels of glycan structure and discuss the potential of microfluidics to gain an improved understanding of those mechanisms.
24596138	6	1	theme	hierarchical	753:764	arg1	levels					766:771	the hierarchical levels	749:771	the hierarchical levels of glycan structure	749:791	Here, we describe the hierarchical levels of glycan structure and discuss the potential of microfluidics to gain an improved understanding of those mechanisms.
24596138	1	2	dep	proteins	101:108	arg1	these					95:99	these	95:99	these	95:99	Proteins play pivotal functions in cells; the study of these proteins and functions is the realm of proteomics.
24596138	4	3	theme	simple	527:532	arg1	glycans					534:540	simple glycans	527:540	simple glycans	527:540	Some aspects of the glycome, including the role that simple glycans and their derivates have in the modulation of cellular behavior, have not yet been elucidated.
24596138	1	4	theme	pivotal	54:60	arg1	functions					62:70	pivotal functions	54:70	pivotal functions	54:70	Proteins play pivotal functions in cells; the study of these proteins and functions is the realm of proteomics.
24596138	1	5	theme	proteomics	140:149	arg1	realm					131:135	the realm	127:135	the realm of proteomics	127:149	Proteins play pivotal functions in cells; the study of these proteins and functions is the realm of proteomics.
24596138	1	5	theme	proteomics	140:149	arg1	study					86:90	the study	82:90	the study of these proteins and functions	82:122	Proteins play pivotal functions in cells; the study of these proteins and functions is the realm of proteomics.
24596138	4	6	theme	behavior	597:604	arg1	modulation					574:583	the modulation	570:583	the modulation of cellular behavior	570:604	Some aspects of the glycome, including the role that simple glycans and their derivates have in the modulation of cellular behavior, have not yet been elucidated.
24596138	6	7	theme	microfluidics	822:834	arg1	potential					809:817	the potential	805:817	the potential of microfluidics to gain an improved understanding of those mechanisms	805:888	Here, we describe the hierarchical levels of glycan structure and discuss the potential of microfluidics to gain an improved understanding of those mechanisms.
24596138	0	8	theme	Micro	0:4	arg1	analysis					6:13	Micro analysis	0:13	Micro analysis	0:13	Micro analysis to map the glycome code.
24596138	4	9	theme	cellular	588:595	arg1	behavior					597:604	cellular behavior	588:604	cellular behavior	588:604	Some aspects of the glycome, including the role that simple glycans and their derivates have in the modulation of cellular behavior, have not yet been elucidated.
24596138	3	10	gly	glycosylation	319:331	arg1	processes					391:399	malignant processes	381:399	malignant processes	381:399	Protein glycosylation and glycan-protein interactions are involved in malignant processes, including those that underlie tumor, diabetes, and Alzheimer's disease.
24596138	3	10	gly	glycosylation	319:331	arg1	those					412:416	those	412:416	those	412:416	Protein glycosylation and glycan-protein interactions are involved in malignant processes, including those that underlie tumor, diabetes, and Alzheimer's disease.
24596138	3	11	theme	malignant	381:389	arg1	processes					391:399	malignant processes	381:399	malignant processes	381:399	Protein glycosylation and glycan-protein interactions are involved in malignant processes, including those that underlie tumor, diabetes, and Alzheimer's disease.
24596138	3	11	theme	malignant	381:389	arg1	those					412:416	those	412:416	those	412:416	Protein glycosylation and glycan-protein interactions are involved in malignant processes, including those that underlie tumor, diabetes, and Alzheimer's disease.
24596138	3	12	theme	glycan-protein	337:350	arg1	interactions					352:363	Protein glycosylation and glycan-protein interactions	311:363	interactions	352:363	Protein glycosylation and glycan-protein interactions are involved in malignant processes, including those that underlie tumor, diabetes, and Alzheimer's disease.
24596138	3	13	theme	Protein	311:317	arg1	glycosylation					319:331	Protein glycosylation and glycan-protein interactions	311:363	glycosylation	319:331	Protein glycosylation and glycan-protein interactions are involved in malignant processes, including those that underlie tumor, diabetes, and Alzheimer's disease.
24596138	4	14	theme	glycome	494:500	arg1	role					517:520	the role	513:520	the role that simple glycans and their derivates have in the modulation of cellular behavior	513:604	Some aspects of the glycome, including the role that simple glycans and their derivates have in the modulation of cellular behavior, have not yet been elucidated.
24596138	4	14	theme	glycome	494:500	arg1	aspects					479:485	Some aspects	474:485	Some aspects	474:485	Some aspects of the glycome, including the role that simple glycans and their derivates have in the modulation of cellular behavior, have not yet been elucidated.
24596138	5	15	theme	Novel	637:641	arg1	technologies					661:672	Novel interdisciplinary technologies	637:672	Novel interdisciplinary technologies	637:672	Novel interdisciplinary technologies are needed to improve our understanding of those fields.
24596138	2	16	theme	immune	249:254	arg1	system					256:261	the immune system	245:261	the immune system	245:261	Less appreciated is that the proteins can be sugar coated and that glycosylation affects how the immune system recognizes the protein, as being friend or foe.
24596138	5	17	theme	fields	723:728	arg1	understanding					700:712	our understanding	696:712	our understanding of those fields	696:728	Novel interdisciplinary technologies are needed to improve our understanding of those fields.
24596138	5	18	theme	interdisciplinary	643:659	arg1	technologies					661:672	Novel interdisciplinary technologies	637:672	Novel interdisciplinary technologies	637:672	Novel interdisciplinary technologies are needed to improve our understanding of those fields.
24596138	0	19	dep	glycome	26:32	arg1	code					34:37	code	34:37	code	34:37	Micro analysis to map the glycome code.
24596138	6	20	theme	mechanisms	879:888	arg1	understanding					856:868	an improved understanding	844:868	an improved understanding of those mechanisms	844:888	Here, we describe the hierarchical levels of glycan structure and discuss the potential of microfluidics to gain an improved understanding of those mechanisms.
24596138	6	21	theme	structure	783:791	arg1	levels					766:771	the hierarchical levels	749:771	the hierarchical levels of glycan structure	749:791	Here, we describe the hierarchical levels of glycan structure and discuss the potential of microfluidics to gain an improved understanding of those mechanisms.
24596138	6	22	theme	glycan	776:781	arg1	structure					783:791	glycan structure	776:791	glycan structure	776:791	Here, we describe the hierarchical levels of glycan structure and discuss the potential of microfluidics to gain an improved understanding of those mechanisms.
24596138	4	23	contain	have	562:565	arg2	role					517:520	the role	513:520	the role that simple glycans and their derivates have in the modulation of cellular behavior	513:604	Some aspects of the glycome, including the role that simple glycans and their derivates have in the modulation of cellular behavior, have not yet been elucidated.
24596138	4	23	contain	have	562:565	arg1	glycans					534:540	simple glycans	527:540	simple glycans	527:540	Some aspects of the glycome, including the role that simple glycans and their derivates have in the modulation of cellular behavior, have not yet been elucidated.
24596138	4	23	contain	have	562:565	arg1	derivates					552:560	their derivates	546:560	their derivates	546:560	Some aspects of the glycome, including the role that simple glycans and their derivates have in the modulation of cellular behavior, have not yet been elucidated.
24596138	1	24	theme	proteins	101:108	arg1	study					86:90	the study	82:90	the study of these proteins and functions	82:122	Proteins play pivotal functions in cells; the study of these proteins and functions is the realm of proteomics.
24596138	1	24	theme	proteins	101:108	arg1	realm					131:135	the realm	127:135	the realm of proteomics	127:149	Proteins play pivotal functions in cells; the study of these proteins and functions is the realm of proteomics.
24596138	1	25	theme	functions	114:122	arg1	study					86:90	the study	82:90	the study of these proteins and functions	82:122	Proteins play pivotal functions in cells; the study of these proteins and functions is the realm of proteomics.
24596138	1	25	theme	functions	114:122	arg1	realm					131:135	the realm	127:135	the realm of proteomics	127:149	Proteins play pivotal functions in cells; the study of these proteins and functions is the realm of proteomics.
26498267	0	0	theme	inflammatory	96:107	arg1	cytokines					109:117	inflammatory cytokines	96:117	inflammatory cytokines	96:117	Fucoidan ameliorates steatohepatitis and insulin resistance by suppressing oxidative stress and inflammatory cytokines in experimental non-alcoholic fatty liver disease.
26498267	5	1	from	decrease	743:750	arg1	glucose					857:863	fasting serum glucose	843:863	fasting serum glucose	843:863	Results showed that administration of fucoidan for 4 weeks attenuated the development of NAFLD as evidenced by the significant decrease in liver index, serum liver enzymes activities, serum total cholesterol and triglycerides, fasting serum glucose, insulin, insulin resistance, and body composition index.
26498267	5	1	from	decrease	743:750	arg1	insulin					866:872	insulin	866:872	insulin	866:872	Results showed that administration of fucoidan for 4 weeks attenuated the development of NAFLD as evidenced by the significant decrease in liver index, serum liver enzymes activities, serum total cholesterol and triglycerides, fasting serum glucose, insulin, insulin resistance, and body composition index.
26498267	5	1	from	decrease	743:750	arg1	activities					788:797	serum liver enzymes activities	768:797	serum liver enzymes activities	768:797	Results showed that administration of fucoidan for 4 weeks attenuated the development of NAFLD as evidenced by the significant decrease in liver index, serum liver enzymes activities, serum total cholesterol and triglycerides, fasting serum glucose, insulin, insulin resistance, and body composition index.
26498267	5	1	from	decrease	743:750	arg1	index					916:920	body composition index	899:920	body composition index	899:920	Results showed that administration of fucoidan for 4 weeks attenuated the development of NAFLD as evidenced by the significant decrease in liver index, serum liver enzymes activities, serum total cholesterol and triglycerides, fasting serum glucose, insulin, insulin resistance, and body composition index.
26498267	5	1	from	decrease	743:750	arg1	index					761:765	liver index	755:765	liver index	755:765	Results showed that administration of fucoidan for 4 weeks attenuated the development of NAFLD as evidenced by the significant decrease in liver index, serum liver enzymes activities, serum total cholesterol and triglycerides, fasting serum glucose, insulin, insulin resistance, and body composition index.
26498267	5	1	from	decrease	743:750	arg1	resistance					883:892	insulin resistance	875:892	insulin resistance	875:892	Results showed that administration of fucoidan for 4 weeks attenuated the development of NAFLD as evidenced by the significant decrease in liver index, serum liver enzymes activities, serum total cholesterol and triglycerides, fasting serum glucose, insulin, insulin resistance, and body composition index.
26498267	5	1	from	decrease	743:750	arg1	cholesterol					812:822	serum total cholesterol	800:822	serum total cholesterol	800:822	Results showed that administration of fucoidan for 4 weeks attenuated the development of NAFLD as evidenced by the significant decrease in liver index, serum liver enzymes activities, serum total cholesterol and triglycerides, fasting serum glucose, insulin, insulin resistance, and body composition index.
26498267	2	2	theme	liver	385:389	arg1	NAFLD					400:404	NAFLD	400:404	NAFLD	400:404	In the present study, we investigated the therapeutic effect of fucoidan on non-alcoholic fatty liver disease (NAFLD) in rats.
26498267	2	2	theme	liver	385:389	arg1	disease					391:397	non-alcoholic fatty liver disease	365:397	non-alcoholic fatty liver disease (NAFLD) in rats	365:413	In the present study, we investigated the therapeutic effect of fucoidan on non-alcoholic fatty liver disease (NAFLD) in rats.
26498267	1	3	theme	properties	277:286	arg1	range					252:256	a wide range	245:256	a wide range of pharmacological properties	245:286	Fucoidan, a sulfated polysaccharide derived from brown seaweeds, possesses a wide range of pharmacological properties.
26498267	5	4	theme	fucoidan	654:661	arg1	administration					636:649	administration	636:649	administration of fucoidan for 4 weeks	636:673	Results showed that administration of fucoidan for 4 weeks attenuated the development of NAFLD as evidenced by the significant decrease in liver index, serum liver enzymes activities, serum total cholesterol and triglycerides, fasting serum glucose, insulin, insulin resistance, and body composition index.
26498267	7	5	from	interleukins-1β	1212:1226	arg1	expressions					1172:1182	hepatic mRNA expressions	1159:1182	hepatic mRNA expressions of tumor necrosis factor-α	1159:1209	In addition, the effect of fucoidan was accompanied with significant decrease in hepatic mRNA expressions of tumor necrosis factor-α, interleukins-1β and matrix metalloproteinase-2.
26498267	1	6	theme	sulfated	182:189	arg1	polysaccharide					191:204	a sulfated polysaccharide	180:204	a sulfated polysaccharide derived from brown seaweeds	180:232	Fucoidan, a sulfated polysaccharide derived from brown seaweeds, possesses a wide range of pharmacological properties.
26498267	1	6	theme	sulfated	182:189	arg1	Fucoidan					170:177	Fucoidan	170:177	Fucoidan	170:177	Fucoidan, a sulfated polysaccharide derived from brown seaweeds, possesses a wide range of pharmacological properties.
26498267	2	7	from	effect	343:348	arg1	NAFLD					400:404	NAFLD	400:404	NAFLD	400:404	In the present study, we investigated the therapeutic effect of fucoidan on non-alcoholic fatty liver disease (NAFLD) in rats.
26498267	2	7	from	effect	343:348	arg1	disease					391:397	non-alcoholic fatty liver disease	365:397	non-alcoholic fatty liver disease (NAFLD) in rats	365:413	In the present study, we investigated the therapeutic effect of fucoidan on non-alcoholic fatty liver disease (NAFLD) in rats.
26498267	6	8	theme	hepatic	1043:1049	arg1	level					1071:1075	hepatic reduced glutathione level	1043:1075	hepatic reduced glutathione level	1043:1075	Further, fucoidan decreased hepatic malondialdehyde as well as nitric oxide concentrations, and concomitantly increased hepatic reduced glutathione level.
26498267	4	9	dep	fucoidan	506:513	arg1	100mg/kg					516:523	100mg/kg	516:523	100mg/kg	516:523	Oral administrations of fucoidan (100mg/kg, orally), metformin (200mg/kg, orally) or the vehicle were started in the last four weeks.
26498267	7	10	theme	mRNA	1167:1170	arg1	expressions					1172:1182	hepatic mRNA expressions	1159:1182	hepatic mRNA expressions of tumor necrosis factor-α	1159:1209	In addition, the effect of fucoidan was accompanied with significant decrease in hepatic mRNA expressions of tumor necrosis factor-α, interleukins-1β and matrix metalloproteinase-2.
26498267	7	11	theme	hepatic	1159:1165	arg1	expressions					1172:1182	hepatic mRNA expressions	1159:1182	hepatic mRNA expressions of tumor necrosis factor-α	1159:1209	In addition, the effect of fucoidan was accompanied with significant decrease in hepatic mRNA expressions of tumor necrosis factor-α, interleukins-1β and matrix metalloproteinase-2.
26498267	4	12	dep	metformin	535:543	arg1	200mg/kg					546:553	200mg/kg	546:553	200mg/kg	546:553	Oral administrations of fucoidan (100mg/kg, orally), metformin (200mg/kg, orally) or the vehicle were started in the last four weeks.
26498267	8	13	theme	fucoidan	1327:1334	arg1	effect					1317:1322	the effect	1313:1322	the effect of fucoidan	1313:1334	Furthermore, histopathological examination confirmed the effect of fucoidan.
26498267	5	14	theme	body	899:902	arg1	index					916:920	body composition index	899:920	body composition index	899:920	Results showed that administration of fucoidan for 4 weeks attenuated the development of NAFLD as evidenced by the significant decrease in liver index, serum liver enzymes activities, serum total cholesterol and triglycerides, fasting serum glucose, insulin, insulin resistance, and body composition index.
26498267	8	15	theme	histopathological	1273:1289	arg1	examination					1291:1301	histopathological examination	1273:1301	histopathological examination	1273:1301	Furthermore, histopathological examination confirmed the effect of fucoidan.
26498267	0	16	theme	non-alcoholic	135:147	arg1	disease					161:167	experimental non-alcoholic fatty liver disease	122:167	experimental non-alcoholic fatty liver disease	122:167	Fucoidan ameliorates steatohepatitis and insulin resistance by suppressing oxidative stress and inflammatory cytokines in experimental non-alcoholic fatty liver disease.
26498267	5	17	theme	serum	851:855	arg1	glucose					857:863	fasting serum glucose	843:863	fasting serum glucose	843:863	Results showed that administration of fucoidan for 4 weeks attenuated the development of NAFLD as evidenced by the significant decrease in liver index, serum liver enzymes activities, serum total cholesterol and triglycerides, fasting serum glucose, insulin, insulin resistance, and body composition index.
26498267	6	18	theme	nitric	986:991	arg1	oxide					993:997	nitric oxide	986:997	hepatic malondialdehyde as well as nitric oxide concentrations	951:1012	Further, fucoidan decreased hepatic malondialdehyde as well as nitric oxide concentrations, and concomitantly increased hepatic reduced glutathione level.
26498267	5	19	theme	composition	904:914	arg1	index					916:920	body composition index	899:920	body composition index	899:920	Results showed that administration of fucoidan for 4 weeks attenuated the development of NAFLD as evidenced by the significant decrease in liver index, serum liver enzymes activities, serum total cholesterol and triglycerides, fasting serum glucose, insulin, insulin resistance, and body composition index.
26498267	0	20	theme	experimental	122:133	arg1	disease					161:167	experimental non-alcoholic fatty liver disease	122:167	experimental non-alcoholic fatty liver disease	122:167	Fucoidan ameliorates steatohepatitis and insulin resistance by suppressing oxidative stress and inflammatory cytokines in experimental non-alcoholic fatty liver disease.
26498267	5	21	theme	fasting	843:849	arg1	glucose					857:863	fasting serum glucose	843:863	fasting serum glucose	843:863	Results showed that administration of fucoidan for 4 weeks attenuated the development of NAFLD as evidenced by the significant decrease in liver index, serum liver enzymes activities, serum total cholesterol and triglycerides, fasting serum glucose, insulin, insulin resistance, and body composition index.
26498267	9	22	theme	sensitizing	1486:1496	arg1	mechanisms					1533:1542	its hypolipidemic, insulin sensitizing, antioxidant and anti-inflammatory mechanisms	1459:1542	its hypolipidemic, insulin sensitizing, antioxidant and anti-inflammatory mechanisms	1459:1542	In conclusion, fucoidan ameliorated the development of HFD-induced NAFLD in rats that may be, at least partly, related to its hypolipidemic, insulin sensitizing, antioxidant and anti-inflammatory mechanisms.
26498267	2	23	theme	present	296:302	arg1	study					304:308	the present study	292:308	the present study	292:308	In the present study, we investigated the therapeutic effect of fucoidan on non-alcoholic fatty liver disease (NAFLD) in rats.
26498267	4	24	theme	last	599:602	arg1	weeks					609:613	the last four weeks	595:613	the last four weeks	595:613	Oral administrations of fucoidan (100mg/kg, orally), metformin (200mg/kg, orally) or the vehicle were started in the last four weeks.
26498267	9	25	theme	antioxidant	1499:1509	arg1	mechanisms					1533:1542	its hypolipidemic, insulin sensitizing, antioxidant and anti-inflammatory mechanisms	1459:1542	its hypolipidemic, insulin sensitizing, antioxidant and anti-inflammatory mechanisms	1459:1542	In conclusion, fucoidan ameliorated the development of HFD-induced NAFLD in rats that may be, at least partly, related to its hypolipidemic, insulin sensitizing, antioxidant and anti-inflammatory mechanisms.
26498267	5	26	theme	total	806:810	arg1	cholesterol					812:822	serum total cholesterol	800:822	serum total cholesterol	800:822	Results showed that administration of fucoidan for 4 weeks attenuated the development of NAFLD as evidenced by the significant decrease in liver index, serum liver enzymes activities, serum total cholesterol and triglycerides, fasting serum glucose, insulin, insulin resistance, and body composition index.
26498267	5	27	theme	liver	755:759	arg1	index					761:765	liver index	755:765	liver index	755:765	Results showed that administration of fucoidan for 4 weeks attenuated the development of NAFLD as evidenced by the significant decrease in liver index, serum liver enzymes activities, serum total cholesterol and triglycerides, fasting serum glucose, insulin, insulin resistance, and body composition index.
26498267	7	28	from	metalloproteinase-2	1239:1257	arg1	expressions					1172:1182	hepatic mRNA expressions	1159:1182	hepatic mRNA expressions of tumor necrosis factor-α	1159:1209	In addition, the effect of fucoidan was accompanied with significant decrease in hepatic mRNA expressions of tumor necrosis factor-α, interleukins-1β and matrix metalloproteinase-2.
26498267	2	29	theme	therapeutic	331:341	arg1	effect					343:348	the therapeutic effect	327:348	the therapeutic effect of fucoidan on non-alcoholic fatty liver disease (NAFLD) in rats	327:413	In the present study, we investigated the therapeutic effect of fucoidan on non-alcoholic fatty liver disease (NAFLD) in rats.
26498267	1	30	theme	brown	219:223	arg1	seaweeds					225:232	brown seaweeds	219:232	brown seaweeds	219:232	Fucoidan, a sulfated polysaccharide derived from brown seaweeds, possesses a wide range of pharmacological properties.
26498267	7	31	theme	fucoidan	1105:1112	arg1	effect					1095:1100	the effect	1091:1100	the effect of fucoidan	1091:1112	In addition, the effect of fucoidan was accompanied with significant decrease in hepatic mRNA expressions of tumor necrosis factor-α, interleukins-1β and matrix metalloproteinase-2.
26498267	0	32	theme	liver	155:159	arg1	disease					161:167	experimental non-alcoholic fatty liver disease	122:167	experimental non-alcoholic fatty liver disease	122:167	Fucoidan ameliorates steatohepatitis and insulin resistance by suppressing oxidative stress and inflammatory cytokines in experimental non-alcoholic fatty liver disease.
26498267	2	33	from	disease	391:397	arg1	rats					410:413	rats	410:413	rats	410:413	In the present study, we investigated the therapeutic effect of fucoidan on non-alcoholic fatty liver disease (NAFLD) in rats.
26498267	9	34	dep	sensitizing	1486:1496	arg1	insulin					1478:1484	insulin	1478:1484	insulin	1478:1484	In conclusion, fucoidan ameliorated the development of HFD-induced NAFLD in rats that may be, at least partly, related to its hypolipidemic, insulin sensitizing, antioxidant and anti-inflammatory mechanisms.
26498267	5	35	theme	serum	768:772	arg1	activities					788:797	serum liver enzymes activities	768:797	serum liver enzymes activities	768:797	Results showed that administration of fucoidan for 4 weeks attenuated the development of NAFLD as evidenced by the significant decrease in liver index, serum liver enzymes activities, serum total cholesterol and triglycerides, fasting serum glucose, insulin, insulin resistance, and body composition index.
26498267	5	36	theme	insulin	875:881	arg1	resistance					883:892	insulin resistance	875:892	insulin resistance	875:892	Results showed that administration of fucoidan for 4 weeks attenuated the development of NAFLD as evidenced by the significant decrease in liver index, serum liver enzymes activities, serum total cholesterol and triglycerides, fasting serum glucose, insulin, insulin resistance, and body composition index.
26498267	4	37	theme	metformin	535:543	arg1	administrations					487:501	Oral administrations	482:501	Oral administrations of fucoidan (100mg/kg, orally), metformin (200mg/kg, orally) or the vehicle	482:577	Oral administrations of fucoidan (100mg/kg, orally), metformin (200mg/kg, orally) or the vehicle were started in the last four weeks.
26498267	0	38	theme	fatty	149:153	arg1	disease					161:167	experimental non-alcoholic fatty liver disease	122:167	experimental non-alcoholic fatty liver disease	122:167	Fucoidan ameliorates steatohepatitis and insulin resistance by suppressing oxidative stress and inflammatory cytokines in experimental non-alcoholic fatty liver disease.
26498267	1	39	attach	derived	206:212	arg2	polysaccharide					191:204	a sulfated polysaccharide	180:204	a sulfated polysaccharide derived from brown seaweeds	180:232	Fucoidan, a sulfated polysaccharide derived from brown seaweeds, possesses a wide range of pharmacological properties.
26498267	1	39	attach	derived	206:212	arg2	Fucoidan					170:177	Fucoidan	170:177	Fucoidan	170:177	Fucoidan, a sulfated polysaccharide derived from brown seaweeds, possesses a wide range of pharmacological properties.
26498267	1	39	attach	derived	206:212	arg1	seaweeds					225:232	brown seaweeds	219:232	brown seaweeds	219:232	Fucoidan, a sulfated polysaccharide derived from brown seaweeds, possesses a wide range of pharmacological properties.
26498267	2	40	theme	fatty	379:383	arg1	NAFLD					400:404	NAFLD	400:404	NAFLD	400:404	In the present study, we investigated the therapeutic effect of fucoidan on non-alcoholic fatty liver disease (NAFLD) in rats.
26498267	2	40	theme	fatty	379:383	arg1	disease					391:397	non-alcoholic fatty liver disease	365:397	non-alcoholic fatty liver disease (NAFLD) in rats	365:413	In the present study, we investigated the therapeutic effect of fucoidan on non-alcoholic fatty liver disease (NAFLD) in rats.
26498267	5	41	theme	liver	774:778	arg1	activities					788:797	serum liver enzymes activities	768:797	serum liver enzymes activities	768:797	Results showed that administration of fucoidan for 4 weeks attenuated the development of NAFLD as evidenced by the significant decrease in liver index, serum liver enzymes activities, serum total cholesterol and triglycerides, fasting serum glucose, insulin, insulin resistance, and body composition index.
26498267	7	42	theme	significant	1135:1145	arg1	decrease					1147:1154	significant decrease	1135:1154	significant decrease in hepatic mRNA expressions of tumor necrosis factor-α	1135:1209	In addition, the effect of fucoidan was accompanied with significant decrease in hepatic mRNA expressions of tumor necrosis factor-α, interleukins-1β and matrix metalloproteinase-2.
26498267	7	43	from	decrease	1147:1154	arg1	expressions					1172:1182	hepatic mRNA expressions	1159:1182	hepatic mRNA expressions of tumor necrosis factor-α	1159:1209	In addition, the effect of fucoidan was accompanied with significant decrease in hepatic mRNA expressions of tumor necrosis factor-α, interleukins-1β and matrix metalloproteinase-2.
26498267	3	44	theme	high-fat	432:439	arg1	diet					441:444	a high-fat diet	430:444	a high-fat diet (HFD)	430:450	Rats were fed a high-fat diet (HFD) for 12 weeks to induce NAFLD.
26498267	3	44	theme	high-fat	432:439	arg1	HFD					447:449	HFD	447:449	HFD	447:449	Rats were fed a high-fat diet (HFD) for 12 weeks to induce NAFLD.
26498267	5	45	theme	enzymes	780:786	arg1	activities					788:797	serum liver enzymes activities	768:797	serum liver enzymes activities	768:797	Results showed that administration of fucoidan for 4 weeks attenuated the development of NAFLD as evidenced by the significant decrease in liver index, serum liver enzymes activities, serum total cholesterol and triglycerides, fasting serum glucose, insulin, insulin resistance, and body composition index.
26498267	0	46	theme	insulin	41:47	arg1	resistance					49:58	insulin resistance	41:58	insulin resistance	41:58	Fucoidan ameliorates steatohepatitis and insulin resistance by suppressing oxidative stress and inflammatory cytokines in experimental non-alcoholic fatty liver disease.
26498267	0	47	from	stress	85:90	arg1	disease					161:167	experimental non-alcoholic fatty liver disease	122:167	experimental non-alcoholic fatty liver disease	122:167	Fucoidan ameliorates steatohepatitis and insulin resistance by suppressing oxidative stress and inflammatory cytokines in experimental non-alcoholic fatty liver disease.
26498267	6	48	theme	hepatic	951:957	arg1	malondialdehyde					959:973	hepatic malondialdehyde	951:973	hepatic malondialdehyde as well as nitric oxide concentrations	951:1012	Further, fucoidan decreased hepatic malondialdehyde as well as nitric oxide concentrations, and concomitantly increased hepatic reduced glutathione level.
26498267	5	49	theme	significant	731:741	arg1	decrease					743:750	the significant decrease	727:750	the significant decrease in liver index, serum liver enzymes activities, serum total cholesterol and triglycerides, fasting serum glucose, insulin, insulin resistance, and body composition index	727:920	Results showed that administration of fucoidan for 4 weeks attenuated the development of NAFLD as evidenced by the significant decrease in liver index, serum liver enzymes activities, serum total cholesterol and triglycerides, fasting serum glucose, insulin, insulin resistance, and body composition index.
26498267	4	50	theme	fucoidan	506:513	arg1	administrations					487:501	Oral administrations	482:501	Oral administrations of fucoidan (100mg/kg, orally), metformin (200mg/kg, orally) or the vehicle	482:577	Oral administrations of fucoidan (100mg/kg, orally), metformin (200mg/kg, orally) or the vehicle were started in the last four weeks.
26498267	5	51	theme	NAFLD	705:709	arg1	development					690:700	the development	686:700	the development of NAFLD	686:709	Results showed that administration of fucoidan for 4 weeks attenuated the development of NAFLD as evidenced by the significant decrease in liver index, serum liver enzymes activities, serum total cholesterol and triglycerides, fasting serum glucose, insulin, insulin resistance, and body composition index.
26498267	2	52	theme	non-alcoholic	365:377	arg1	NAFLD					400:404	NAFLD	400:404	NAFLD	400:404	In the present study, we investigated the therapeutic effect of fucoidan on non-alcoholic fatty liver disease (NAFLD) in rats.
26498267	2	52	theme	non-alcoholic	365:377	arg1	disease					391:397	non-alcoholic fatty liver disease	365:397	non-alcoholic fatty liver disease (NAFLD) in rats	365:413	In the present study, we investigated the therapeutic effect of fucoidan on non-alcoholic fatty liver disease (NAFLD) in rats.
26498267	9	53	theme	hypolipidemic	1463:1475	arg1	mechanisms					1533:1542	its hypolipidemic, insulin sensitizing, antioxidant and anti-inflammatory mechanisms	1459:1542	its hypolipidemic, insulin sensitizing, antioxidant and anti-inflammatory mechanisms	1459:1542	In conclusion, fucoidan ameliorated the development of HFD-induced NAFLD in rats that may be, at least partly, related to its hypolipidemic, insulin sensitizing, antioxidant and anti-inflammatory mechanisms.
26498267	9	54	from	development	1377:1387	arg1	rats					1413:1416	rats	1413:1416	rats that may be, at least partly, related to its hypolipidemic, insulin sensitizing, antioxidant and anti-inflammatory mechanisms	1413:1542	In conclusion, fucoidan ameliorated the development of HFD-induced NAFLD in rats that may be, at least partly, related to its hypolipidemic, insulin sensitizing, antioxidant and anti-inflammatory mechanisms.
26498267	1	55	contain	possesses	235:243	arg1	polysaccharide					191:204	a sulfated polysaccharide	180:204	a sulfated polysaccharide derived from brown seaweeds	180:232	Fucoidan, a sulfated polysaccharide derived from brown seaweeds, possesses a wide range of pharmacological properties.
26498267	1	55	contain	possesses	235:243	arg2	range					252:256	a wide range	245:256	a wide range of pharmacological properties	245:286	Fucoidan, a sulfated polysaccharide derived from brown seaweeds, possesses a wide range of pharmacological properties.
26498267	1	55	contain	possesses	235:243	arg1	Fucoidan					170:177	Fucoidan	170:177	Fucoidan	170:177	Fucoidan, a sulfated polysaccharide derived from brown seaweeds, possesses a wide range of pharmacological properties.
26498267	2	56	theme	fucoidan	353:360	arg1	effect					343:348	the therapeutic effect	327:348	the therapeutic effect of fucoidan on non-alcoholic fatty liver disease (NAFLD) in rats	327:413	In the present study, we investigated the therapeutic effect of fucoidan on non-alcoholic fatty liver disease (NAFLD) in rats.
26498267	1	57	theme	wide	247:250	arg1	range					252:256	a wide range	245:256	a wide range of pharmacological properties	245:286	Fucoidan, a sulfated polysaccharide derived from brown seaweeds, possesses a wide range of pharmacological properties.
26498267	9	58	theme	anti-inflammatory	1515:1531	arg1	mechanisms					1533:1542	its hypolipidemic, insulin sensitizing, antioxidant and anti-inflammatory mechanisms	1459:1542	its hypolipidemic, insulin sensitizing, antioxidant and anti-inflammatory mechanisms	1459:1542	In conclusion, fucoidan ameliorated the development of HFD-induced NAFLD in rats that may be, at least partly, related to its hypolipidemic, insulin sensitizing, antioxidant and anti-inflammatory mechanisms.
26498267	9	59	theme	HFD-induced	1392:1402	arg1	NAFLD					1404:1408	HFD-induced NAFLD	1392:1408	HFD-induced NAFLD	1392:1408	In conclusion, fucoidan ameliorated the development of HFD-induced NAFLD in rats that may be, at least partly, related to its hypolipidemic, insulin sensitizing, antioxidant and anti-inflammatory mechanisms.
26498267	7	60	theme	matrix	1232:1237	arg1	metalloproteinase-2					1239:1257	matrix metalloproteinase-2	1232:1257	matrix metalloproteinase-2	1232:1257	In addition, the effect of fucoidan was accompanied with significant decrease in hepatic mRNA expressions of tumor necrosis factor-α, interleukins-1β and matrix metalloproteinase-2.
26498267	7	61	theme	tumor	1187:1191	arg1	necrosis					1193:1200	tumor necrosis factor-α	1187:1209	tumor necrosis factor-α	1187:1209	In addition, the effect of fucoidan was accompanied with significant decrease in hepatic mRNA expressions of tumor necrosis factor-α, interleukins-1β and matrix metalloproteinase-2.
26498267	0	62	from	cytokines	109:117	arg1	disease					161:167	experimental non-alcoholic fatty liver disease	122:167	experimental non-alcoholic fatty liver disease	122:167	Fucoidan ameliorates steatohepatitis and insulin resistance by suppressing oxidative stress and inflammatory cytokines in experimental non-alcoholic fatty liver disease.
26498267	6	63	theme	oxide	993:997	arg1	concentrations					999:1012	nitric oxide concentrations	986:1012	hepatic malondialdehyde as well as nitric oxide concentrations	951:1012	Further, fucoidan decreased hepatic malondialdehyde as well as nitric oxide concentrations, and concomitantly increased hepatic reduced glutathione level.
26498267	6	64	theme	glutathione	1059:1069	arg1	level					1071:1075	hepatic reduced glutathione level	1043:1075	hepatic reduced glutathione level	1043:1075	Further, fucoidan decreased hepatic malondialdehyde as well as nitric oxide concentrations, and concomitantly increased hepatic reduced glutathione level.
26498267	5	65	theme	serum	800:804	arg1	cholesterol					812:822	serum total cholesterol	800:822	serum total cholesterol	800:822	Results showed that administration of fucoidan for 4 weeks attenuated the development of NAFLD as evidenced by the significant decrease in liver index, serum liver enzymes activities, serum total cholesterol and triglycerides, fasting serum glucose, insulin, insulin resistance, and body composition index.
26498267	4	66	theme	vehicle	571:577	arg1	administrations					487:501	Oral administrations	482:501	Oral administrations of fucoidan (100mg/kg, orally), metformin (200mg/kg, orally) or the vehicle	482:577	Oral administrations of fucoidan (100mg/kg, orally), metformin (200mg/kg, orally) or the vehicle were started in the last four weeks.
26498267	0	67	theme	oxidative	75:83	arg1	stress					85:90	oxidative stress	75:90	oxidative stress	75:90	Fucoidan ameliorates steatohepatitis and insulin resistance by suppressing oxidative stress and inflammatory cytokines in experimental non-alcoholic fatty liver disease.
26498267	4	68	theme	Oral	482:485	arg1	administrations					487:501	Oral administrations	482:501	Oral administrations of fucoidan (100mg/kg, orally), metformin (200mg/kg, orally) or the vehicle	482:577	Oral administrations of fucoidan (100mg/kg, orally), metformin (200mg/kg, orally) or the vehicle were started in the last four weeks.
26498267	9	69	theme	NAFLD	1404:1408	arg1	development					1377:1387	the development	1373:1387	the development of HFD-induced NAFLD in rats that may be, at least partly, related to its hypolipidemic, insulin sensitizing, antioxidant and anti-inflammatory mechanisms	1373:1542	In conclusion, fucoidan ameliorated the development of HFD-induced NAFLD in rats that may be, at least partly, related to its hypolipidemic, insulin sensitizing, antioxidant and anti-inflammatory mechanisms.
26498267	6	70	theme	reduced	1051:1057	arg1	level					1071:1075	hepatic reduced glutathione level	1043:1075	hepatic reduced glutathione level	1043:1075	Further, fucoidan decreased hepatic malondialdehyde as well as nitric oxide concentrations, and concomitantly increased hepatic reduced glutathione level.
26498267	7	71	theme	necrosis	1193:1200	arg1	expressions					1172:1182	hepatic mRNA expressions	1159:1182	hepatic mRNA expressions of tumor necrosis factor-α	1159:1209	In addition, the effect of fucoidan was accompanied with significant decrease in hepatic mRNA expressions of tumor necrosis factor-α, interleukins-1β and matrix metalloproteinase-2.
26498267	1	72	theme	pharmacological	261:275	arg1	properties					277:286	pharmacological properties	261:286	pharmacological properties	261:286	Fucoidan, a sulfated polysaccharide derived from brown seaweeds, possesses a wide range of pharmacological properties.
26109728	14	0	gly	glycoprotein	2211:2222	arg1	glycoprotein					2211:2222	the virus envelope glycoprotein	2192:2222	the virus envelope glycoprotein that can induce neutralizing antibodies	2192:2262	The V3 loop is one of the few immunogenic targets on the virus envelope glycoprotein that can induce neutralizing antibodies, but in many viruses, parts of V3 are inaccessible for antibody recognition.
26109728	8	1	theme	Fab	1155:1157	arg1	structure					1137:1145	the crystal structure	1125:1145	the crystal structure of 2424 Fab in complex with a JRFL V3 peptide	1125:1191	To understand the distinct activity of 2424, we determined the crystal structure of 2424 Fab in complex with a JRFL V3 peptide and showed that the 2424 epitope is located at the tip of the V3 crown ((307)IHIGPGRAFYT(319)), dominated by interactions with His(P308), Pro(P313), and Arg(P315).
26109728	14	2	theme	many	2272:2275	arg1	viruses					2277:2283	many viruses	2272:2283	many viruses	2272:2283	The V3 loop is one of the few immunogenic targets on the virus envelope glycoprotein that can induce neutralizing antibodies, but in many viruses, parts of V3 are inaccessible for antibody recognition.
26109728	14	3	theme	immunogenic	2169:2179	arg1	targets					2181:2187	the few immunogenic targets	2161:2187	the few immunogenic targets on the virus envelope glycoprotein that can induce neutralizing antibodies	2161:2262	The V3 loop is one of the few immunogenic targets on the virus envelope glycoprotein that can induce neutralizing antibodies, but in many viruses, parts of V3 are inaccessible for antibody recognition.
26109728	13	4	theme	scientific	1973:1982	arg1	challenges					1984:1993	significant scientific challenges	1961:1993	significant scientific challenges	1961:1993	However, HIV vaccine development has been hampered by significant scientific challenges, one of which is the inability of HIV vaccine candidates evaluated thus far to elicit production of potent and broadly neutralizing antibodies.
26109728	16	5	theme	other	2588:2592	arg1	parts					2594:2598	other parts	2588:2598	other parts of V3	2588:2604	Our data reveal that this antibody recognizes the most distal tip of V3, which is not as occluded as other parts of V3.
26109728	2	6	theme	better	402:407	arg1	understanding					409:421	a better understanding	400:421	a better understanding of these antibodies	400:441	Although most anti-V3 antibodies neutralize only the sensitive tier 1 viruses, anti-V3 antibodies effective against the more resistant viruses exist, and a better understanding of these antibodies and their epitopes would be beneficial for the development of novel vaccine immunogens against HIV.
26109728	2	6	theme	better	402:407	arg1	beneficial					471:480	beneficial	471:480	beneficial	471:480	Although most anti-V3 antibodies neutralize only the sensitive tier 1 viruses, anti-V3 antibodies effective against the more resistant viruses exist, and a better understanding of these antibodies and their epitopes would be beneficial for the development of novel vaccine immunogens against HIV.
26109728	4	7	theme	MAb	665:667	arg1	2424					669:672	the V3 MAb 2424	658:672	the V3 MAb 2424	658:672	However, the V3 MAb 2424 achieves 100% neutralization against JRFL.
26109728	2	8	theme	sensitive	299:307	arg1	viruses					316:322	only the sensitive tier 1 viruses	290:322	only the sensitive tier 1 viruses	290:322	Although most anti-V3 antibodies neutralize only the sensitive tier 1 viruses, anti-V3 antibodies effective against the more resistant viruses exist, and a better understanding of these antibodies and their epitopes would be beneficial for the development of novel vaccine immunogens against HIV.
26109728	11	9	theme	2424	1623:1626	arg1	epitope					1628:1634	the 2424 epitope	1619:1634	the 2424 epitope	1619:1634	This feature distinguishes the 2424 epitope from the other V3 crown epitopes and indicates that the tip of V3 is a potential site to target and incorporate into HIV vaccine immunogens.
26109728	1	10	theme	virus-coreceptor	193:208	arg1	interaction					210:220	virus-coreceptor interaction	193:220	virus-coreceptor interaction	193:220	UNLABELLED The V3 region of HIV-1 gp120 is important for virus-coreceptor interaction and highly immunogenic.
26109728	12	11	theme	HIV/AIDS	1788:1795	arg1	vaccines					1797:1804	IMPORTANCE HIV/AIDS vaccines	1777:1804	IMPORTANCE HIV/AIDS vaccines	1777:1804	IMPORTANCE HIV/AIDS vaccines are crucial for controlling the HIV epidemics that continue to afflict millions of people worldwide.
26109728	13	12	theme	vaccine	1920:1926	arg1	development					1928:1938	HIV vaccine development	1916:1938	HIV vaccine development	1916:1938	However, HIV vaccine development has been hampered by significant scientific challenges, one of which is the inability of HIV vaccine candidates evaluated thus far to elicit production of potent and broadly neutralizing antibodies.
26109728	11	13	theme	other	1645:1649	arg1	epitopes					1660:1667	the other V3 crown epitopes	1641:1667	the other V3 crown epitopes	1641:1667	This feature distinguishes the 2424 epitope from the other V3 crown epitopes and indicates that the tip of V3 is a potential site to target and incorporate into HIV vaccine immunogens.
26109728	11	14	theme	vaccine	1757:1763	arg1	immunogens					1765:1774	HIV vaccine immunogens	1753:1774	HIV vaccine immunogens	1753:1774	This feature distinguishes the 2424 epitope from the other V3 crown epitopes and indicates that the tip of V3 is a potential site to target and incorporate into HIV vaccine immunogens.
26109728	13	15	theme	antibodies	2127:2136	arg1	production					2081:2090	production	2081:2090	production of potent and broadly neutralizing antibodies	2081:2136	However, HIV vaccine development has been hampered by significant scientific challenges, one of which is the inability of HIV vaccine candidates evaluated thus far to elicit production of potent and broadly neutralizing antibodies.
26109728	13	16	theme	HIV	2029:2031	arg1	candidates					2041:2050	HIV vaccine candidates	2029:2050	HIV vaccine candidates evaluated thus far to elicit production of potent and broadly neutralizing antibodies	2029:2136	However, HIV vaccine development has been hampered by significant scientific challenges, one of which is the inability of HIV vaccine candidates evaluated thus far to elicit production of potent and broadly neutralizing antibodies.
26109728	16	17	theme	V3	2556:2557	arg1	tip					2549:2551	the most distal tip	2533:2551	the most distal tip	2533:2551	Our data reveal that this antibody recognizes the most distal tip of V3, which is not as occluded as other parts of V3.
26109728	7	18	theme	virus	1050:1054	arg1	envelope					1056:1063	the virus envelope	1046:1063	the virus envelope	1046:1063	Studies of 2424-mediated neutralization of JRFL produced with a mannosidase inhibitor further revealed that its neutralizing activity is unaffected by the glycan composition of the virus envelope.
26109728	1	19	theme	UNLABELLED	136:145	arg1	region					154:159	UNLABELLED The V3 region	136:159	UNLABELLED The V3 region of HIV-1 gp120	136:174	UNLABELLED The V3 region of HIV-1 gp120 is important for virus-coreceptor interaction and highly immunogenic.
26109728	8	20	theme	crown	1258:1262	arg1	tip					1244:1246	the tip	1240:1246	the tip of the V3 crown ((307)IHIGPGRAFYT(319))	1240:1286	To understand the distinct activity of 2424, we determined the crystal structure of 2424 Fab in complex with a JRFL V3 peptide and showed that the 2424 epitope is located at the tip of the V3 crown ((307)IHIGPGRAFYT(319)), dominated by interactions with His(P308), Pro(P313), and Arg(P315).
26109728	14	21	theme	V3	2143:2144	arg1	targets					2181:2187	the few immunogenic targets	2161:2187	the few immunogenic targets on the virus envelope glycoprotein that can induce neutralizing antibodies	2161:2262	The V3 loop is one of the few immunogenic targets on the virus envelope glycoprotein that can induce neutralizing antibodies, but in many viruses, parts of V3 are inaccessible for antibody recognition.
26109728	14	21	theme	V3	2143:2144	arg1	one					2154:2156	one	2154:2156	one	2154:2156	The V3 loop is one of the few immunogenic targets on the virus envelope glycoprotein that can induce neutralizing antibodies, but in many viruses, parts of V3 are inaccessible for antibody recognition.
26109728	14	21	theme	V3	2143:2144	arg1	loop					2146:2149	The V3 loop	2139:2149	The V3 loop	2139:2149	The V3 loop is one of the few immunogenic targets on the virus envelope glycoprotein that can induce neutralizing antibodies, but in many viruses, parts of V3 are inaccessible for antibody recognition.
26109728	8	22	from	tip	1244:1246	arg1	epitope					1218:1224	the 2424 epitope	1209:1224	the 2424 epitope	1209:1224	To understand the distinct activity of 2424, we determined the crystal structure of 2424 Fab in complex with a JRFL V3 peptide and showed that the 2424 epitope is located at the tip of the V3 crown ((307)IHIGPGRAFYT(319)), dominated by interactions with His(P308), Pro(P313), and Arg(P315).
26109728	8	22	from	tip	1244:1246	arg1	dominated					1289:1297	dominated	1289:1297	dominated by interactions with His(P308), Pro(P313), and Arg(P315)	1289:1354	To understand the distinct activity of 2424, we determined the crystal structure of 2424 Fab in complex with a JRFL V3 peptide and showed that the 2424 epitope is located at the tip of the V3 crown ((307)IHIGPGRAFYT(319)), dominated by interactions with His(P308), Pro(P313), and Arg(P315).
26109728	8	22	from	tip	1244:1246	arg1	located					1229:1235	located	1229:1235	located	1229:1235	To understand the distinct activity of 2424, we determined the crystal structure of 2424 Fab in complex with a JRFL V3 peptide and showed that the 2424 epitope is located at the tip of the V3 crown ((307)IHIGPGRAFYT(319)), dominated by interactions with His(P308), Pro(P313), and Arg(P315).
26109728	9	23	theme	binding	1361:1367	arg1	similar					1385:1391	similar	1385:1391	similar	1385:1391	The binding mode of 2424 is similar to that of the well-characterized MAb 447-52D, although 2424 is more side chain dependent.
26109728	9	23	theme	binding	1361:1367	arg1	mode					1369:1372	The binding mode	1357:1372	The binding mode of 2424	1357:1380	The binding mode of 2424 is similar to that of the well-characterized MAb 447-52D, although 2424 is more side chain dependent.
26109728	12	24	theme	worldwide	1896:1904	arg1	millions					1877:1884	millions	1877:1884	millions of people worldwide	1877:1904	IMPORTANCE HIV/AIDS vaccines are crucial for controlling the HIV epidemics that continue to afflict millions of people worldwide.
26109728	17	25	theme	sites	2662:2666	arg1	one					2640:2642	one	2640:2642	one	2640:2642	Hence, the epitope of 2424 is in one of the vulnerable sites on the virus that may be exploited in designing HIV vaccine immunogens.
26109728	17	25	theme	sites	2662:2666	arg1	sites					2662:2666	the vulnerable sites	2647:2666	the vulnerable sites	2647:2666	Hence, the epitope of 2424 is in one of the vulnerable sites on the virus that may be exploited in designing HIV vaccine immunogens.
26109728	4	26	theme	%	686:686	arg1	neutralization					688:701	100% neutralization	683:701	100% neutralization against JRFL	683:714	However, the V3 MAb 2424 achieves 100% neutralization against JRFL.
26109728	10	27	from	glycans	1553:1559	arg1	apex					1524:1527	the very apex	1515:1527	the very apex of V3, away from nearby glycans, facilitating antibody access	1515:1589	The 2424 epitope is focused on the very apex of V3, away from nearby glycans, facilitating antibody access.
26109728	0	28	theme	Antibody	75:82	arg1	Characterization					26:41	Functional and Structural Characterization	0:41	Functional and Structural Characterization of Human V3-Specific Monoclonal Antibody 2424 with Neutralizing Activity against HIV-1 JRFL.	0:134	Functional and Structural Characterization of Human V3-Specific Monoclonal Antibody 2424 with Neutralizing Activity against HIV-1 JRFL.
26109728	17	29	theme	vaccine	2720:2726	arg1	immunogens					2728:2737	HIV vaccine immunogens	2716:2737	HIV vaccine immunogens	2716:2737	Hence, the epitope of 2424 is in one of the vulnerable sites on the virus that may be exploited in designing HIV vaccine immunogens.
26109728	17	30	from	one	2640:2642	arg1	virus					2675:2679	the virus	2671:2679	the virus that may be exploited in designing HIV vaccine immunogens	2671:2737	Hence, the epitope of 2424 is in one of the vulnerable sites on the virus that may be exploited in designing HIV vaccine immunogens.
26109728	2	31	theme	vaccine	511:517	arg1	immunogens					519:528	novel vaccine immunogens	505:528	novel vaccine immunogens against HIV	505:540	Although most anti-V3 antibodies neutralize only the sensitive tier 1 viruses, anti-V3 antibodies effective against the more resistant viruses exist, and a better understanding of these antibodies and their epitopes would be beneficial for the development of novel vaccine immunogens against HIV.
26109728	14	32	from	viruses	2277:2283	arg1	inaccessible					2302:2313	inaccessible	2302:2313	inaccessible	2302:2313	The V3 loop is one of the few immunogenic targets on the virus envelope glycoprotein that can induce neutralizing antibodies, but in many viruses, parts of V3 are inaccessible for antibody recognition.
26109728	1	33	theme	gp120	170:174	arg1	region					154:159	UNLABELLED The V3 region	136:159	UNLABELLED The V3 region of HIV-1 gp120	136:174	UNLABELLED The V3 region of HIV-1 gp120 is important for virus-coreceptor interaction and highly immunogenic.
26109728	12	34	theme	people	1889:1894	arg1	worldwide					1896:1904	people worldwide	1889:1904	people worldwide	1889:1904	IMPORTANCE HIV/AIDS vaccines are crucial for controlling the HIV epidemics that continue to afflict millions of people worldwide.
26109728	15	35	theme	most	2467:2470	arg1	antibodies					2475:2484	most V3 antibodies	2467:2484	most V3 antibodies	2467:2484	This study examined a V3-specific monoclonal antibody that can completely neutralize HIV-1 JRFL, a virus isolate resistant to most V3 antibodies.
26109728	1	36	theme	V3	151:152	arg1	region					154:159	UNLABELLED The V3 region	136:159	UNLABELLED The V3 region of HIV-1 gp120	136:174	UNLABELLED The V3 region of HIV-1 gp120 is important for virus-coreceptor interaction and highly immunogenic.
26109728	15	37	theme	resistant	2454:2462	arg1	isolate					2446:2452	a virus isolate	2438:2452	a virus isolate resistant to most V3 antibodies	2438:2484	This study examined a V3-specific monoclonal antibody that can completely neutralize HIV-1 JRFL, a virus isolate resistant to most V3 antibodies.
26109728	15	37	theme	resistant	2454:2462	arg1	HIV-1					2426:2430	HIV-1 JRFL, a virus isolate resistant to most V3 antibodies	2426:2484	HIV-1	2426:2430	This study examined a V3-specific monoclonal antibody that can completely neutralize HIV-1 JRFL, a virus isolate resistant to most V3 antibodies.
26109728	0	38	theme	Human	46:50	arg1	Antibody					75:82	Human V3-Specific Monoclonal Antibody 2424	46:87	Human V3-Specific Monoclonal Antibody 2424 with Neutralizing Activity against HIV-1 JRFL	46:133	Functional and Structural Characterization of Human V3-Specific Monoclonal Antibody 2424 with Neutralizing Activity against HIV-1 JRFL.
26109728	8	39	dep	located	1229:1235	arg1	epitope					1218:1224	the 2424 epitope	1209:1224	the 2424 epitope	1209:1224	To understand the distinct activity of 2424, we determined the crystal structure of 2424 Fab in complex with a JRFL V3 peptide and showed that the 2424 epitope is located at the tip of the V3 crown ((307)IHIGPGRAFYT(319)), dominated by interactions with His(P308), Pro(P313), and Arg(P315).
26109728	8	39	dep	located	1229:1235	arg1	dominated					1289:1297	dominated	1289:1297	dominated by interactions with His(P308), Pro(P313), and Arg(P315)	1289:1354	To understand the distinct activity of 2424, we determined the crystal structure of 2424 Fab in complex with a JRFL V3 peptide and showed that the 2424 epitope is located at the tip of the V3 crown ((307)IHIGPGRAFYT(319)), dominated by interactions with His(P308), Pro(P313), and Arg(P315).
26109728	8	39	dep	located	1229:1235	arg1	located					1229:1235	located	1229:1235	located	1229:1235	To understand the distinct activity of 2424, we determined the crystal structure of 2424 Fab in complex with a JRFL V3 peptide and showed that the 2424 epitope is located at the tip of the V3 crown ((307)IHIGPGRAFYT(319)), dominated by interactions with His(P308), Pro(P313), and Arg(P315).
26109728	11	40	theme	V3	1699:1700	arg1	tip					1692:1694	the tip	1688:1694	the tip of V3	1688:1700	This feature distinguishes the 2424 epitope from the other V3 crown epitopes and indicates that the tip of V3 is a potential site to target and incorporate into HIV vaccine immunogens.
26109728	11	40	theme	V3	1699:1700	arg1	site					1717:1720	a potential site	1705:1720	a potential site to target and incorporate into HIV vaccine immunogens	1705:1774	This feature distinguishes the 2424 epitope from the other V3 crown epitopes and indicates that the tip of V3 is a potential site to target and incorporate into HIV vaccine immunogens.
26109728	0	41	theme	Functional	0:9	arg1	Characterization					26:41	Functional and Structural Characterization	0:41	Functional and Structural Characterization of Human V3-Specific Monoclonal Antibody 2424 with Neutralizing Activity against HIV-1 JRFL.	0:134	Functional and Structural Characterization of Human V3-Specific Monoclonal Antibody 2424 with Neutralizing Activity against HIV-1 JRFL.
26109728	9	42	theme	well-characterized	1408:1425	arg1	447-52D					1431:1437	the well-characterized MAb 447-52D	1404:1437	the well-characterized MAb 447-52D	1404:1437	The binding mode of 2424 is similar to that of the well-characterized MAb 447-52D, although 2424 is more side chain dependent.
26109728	14	43	theme	few	2165:2167	arg1	targets					2181:2187	the few immunogenic targets	2161:2187	the few immunogenic targets on the virus envelope glycoprotein that can induce neutralizing antibodies	2161:2262	The V3 loop is one of the few immunogenic targets on the virus envelope glycoprotein that can induce neutralizing antibodies, but in many viruses, parts of V3 are inaccessible for antibody recognition.
26109728	16	44	theme	distal	2542:2547	arg1	tip					2549:2551	the most distal tip	2533:2551	the most distal tip	2533:2551	Our data reveal that this antibody recognizes the most distal tip of V3, which is not as occluded as other parts of V3.
26109728	0	45	theme	Structural	15:24	arg1	Characterization					26:41	Functional and Structural Characterization	0:41	Functional and Structural Characterization of Human V3-Specific Monoclonal Antibody 2424 with Neutralizing Activity against HIV-1 JRFL.	0:134	Functional and Structural Characterization of Human V3-Specific Monoclonal Antibody 2424 with Neutralizing Activity against HIV-1 JRFL.
26109728	13	46	theme	vaccine	2033:2039	arg1	candidates					2041:2050	HIV vaccine candidates	2029:2050	HIV vaccine candidates evaluated thus far to elicit production of potent and broadly neutralizing antibodies	2029:2136	However, HIV vaccine development has been hampered by significant scientific challenges, one of which is the inability of HIV vaccine candidates evaluated thus far to elicit production of potent and broadly neutralizing antibodies.
26109728	6	47	theme	neutralization	844:857	arg1	profiles					859:866	distinct binding and neutralization profiles	823:866	profiles	859:866	2424 also has distinct binding and neutralization profiles.
26109728	2	48	theme	antibodies	432:441	arg1	understanding					409:421	a better understanding	400:421	a better understanding of these antibodies	400:441	Although most anti-V3 antibodies neutralize only the sensitive tier 1 viruses, anti-V3 antibodies effective against the more resistant viruses exist, and a better understanding of these antibodies and their epitopes would be beneficial for the development of novel vaccine immunogens against HIV.
26109728	2	48	theme	antibodies	432:441	arg1	epitopes					453:460	their epitopes	447:460	their epitopes	447:460	Although most anti-V3 antibodies neutralize only the sensitive tier 1 viruses, anti-V3 antibodies effective against the more resistant viruses exist, and a better understanding of these antibodies and their epitopes would be beneficial for the development of novel vaccine immunogens against HIV.
26109728	2	48	theme	antibodies	432:441	arg1	beneficial					471:480	beneficial	471:480	beneficial	471:480	Although most anti-V3 antibodies neutralize only the sensitive tier 1 viruses, anti-V3 antibodies effective against the more resistant viruses exist, and a better understanding of these antibodies and their epitopes would be beneficial for the development of novel vaccine immunogens against HIV.
26109728	14	49	theme	antibody	2319:2326	arg1	recognition					2328:2338	antibody recognition	2319:2338	antibody recognition	2319:2338	The V3 loop is one of the few immunogenic targets on the virus envelope glycoprotein that can induce neutralizing antibodies, but in many viruses, parts of V3 are inaccessible for antibody recognition.
26109728	10	50	theme	V3	1532:1533	arg1	apex					1524:1527	the very apex	1515:1527	the very apex of V3, away from nearby glycans, facilitating antibody access	1515:1589	The 2424 epitope is focused on the very apex of V3, away from nearby glycans, facilitating antibody access.
26109728	0	51	theme	V3-Specific	52:62	arg1	Antibody					75:82	Human V3-Specific Monoclonal Antibody 2424	46:87	Human V3-Specific Monoclonal Antibody 2424 with Neutralizing Activity against HIV-1 JRFL	46:133	Functional and Structural Characterization of Human V3-Specific Monoclonal Antibody 2424 with Neutralizing Activity against HIV-1 JRFL.
26109728	8	52	theme	2424	1105:1108	arg1	activity					1093:1100	the distinct activity	1080:1100	the distinct activity of 2424	1080:1108	To understand the distinct activity of 2424, we determined the crystal structure of 2424 Fab in complex with a JRFL V3 peptide and showed that the 2424 epitope is located at the tip of the V3 crown ((307)IHIGPGRAFYT(319)), dominated by interactions with His(P308), Pro(P313), and Arg(P315).
26109728	14	53	from	inaccessible	2302:2313	arg1	viruses					2277:2283	many viruses	2272:2283	many viruses	2272:2283	The V3 loop is one of the few immunogenic targets on the virus envelope glycoprotein that can induce neutralizing antibodies, but in many viruses, parts of V3 are inaccessible for antibody recognition.
26109728	11	54	theme	crown	1654:1658	arg1	epitopes					1660:1667	the other V3 crown epitopes	1641:1667	the other V3 crown epitopes	1641:1667	This feature distinguishes the 2424 epitope from the other V3 crown epitopes and indicates that the tip of V3 is a potential site to target and incorporate into HIV vaccine immunogens.
26109728	7	55	theme	JRFL	912:915	arg1	neutralization					894:907	2424-mediated neutralization	880:907	2424-mediated neutralization of JRFL produced with a mannosidase inhibitor	880:953	Studies of 2424-mediated neutralization of JRFL produced with a mannosidase inhibitor further revealed that its neutralizing activity is unaffected by the glycan composition of the virus envelope.
26109728	13	56	theme	challenges	1984:1993	arg1	inability					2016:2024	the inability	2012:2024	the inability of HIV vaccine candidates evaluated thus far to elicit production of potent and broadly neutralizing antibodies	2012:2136	However, HIV vaccine development has been hampered by significant scientific challenges, one of which is the inability of HIV vaccine candidates evaluated thus far to elicit production of potent and broadly neutralizing antibodies.
26109728	13	56	theme	challenges	1984:1993	arg1	one					1996:1998	one	1996:1998	one	1996:1998	However, HIV vaccine development has been hampered by significant scientific challenges, one of which is the inability of HIV vaccine candidates evaluated thus far to elicit production of potent and broadly neutralizing antibodies.
26109728	13	56	theme	challenges	1984:1993	arg1	challenges					1984:1993	significant scientific challenges	1961:1993	significant scientific challenges	1961:1993	However, HIV vaccine development has been hampered by significant scientific challenges, one of which is the inability of HIV vaccine candidates evaluated thus far to elicit production of potent and broadly neutralizing antibodies.
26109728	11	57	theme	HIV	1753:1755	arg1	immunogens					1765:1774	HIV vaccine immunogens	1753:1774	HIV vaccine immunogens	1753:1774	This feature distinguishes the 2424 epitope from the other V3 crown epitopes and indicates that the tip of V3 is a potential site to target and incorporate into HIV vaccine immunogens.
26109728	9	58	theme	side	1462:1465	arg1	chain					1467:1471	more side chain	1457:1471	more side chain	1457:1471	The binding mode of 2424 is similar to that of the well-characterized MAb 447-52D, although 2424 is more side chain dependent.
26109728	14	59	theme	envelope	2202:2209	arg1	glycoprotein					2211:2222	the virus envelope glycoprotein	2192:2222	the virus envelope glycoprotein that can induce neutralizing antibodies	2192:2262	The V3 loop is one of the few immunogenic targets on the virus envelope glycoprotein that can induce neutralizing antibodies, but in many viruses, parts of V3 are inaccessible for antibody recognition.
26109728	10	60	theme	nearby	1546:1551	arg1	glycans					1553:1559	nearby glycans	1546:1559	nearby glycans	1546:1559	The 2424 epitope is focused on the very apex of V3, away from nearby glycans, facilitating antibody access.
26109728	7	61	theme	neutralization	894:907	arg1	Studies					869:875	Studies	869:875	Studies of 2424-mediated neutralization of JRFL produced with a mannosidase inhibitor	869:953	Studies of 2424-mediated neutralization of JRFL produced with a mannosidase inhibitor further revealed that its neutralizing activity is unaffected by the glycan composition of the virus envelope.
26109728	15	62	theme	V3	2472:2473	arg1	antibodies					2475:2484	most V3 antibodies	2467:2484	most V3 antibodies	2467:2484	This study examined a V3-specific monoclonal antibody that can completely neutralize HIV-1 JRFL, a virus isolate resistant to most V3 antibodies.
26109728	15	63	dep	HIV-1	2426:2430	arg1	JRFL					2432:2435	JRFL	2432:2435	JRFL	2432:2435	This study examined a V3-specific monoclonal antibody that can completely neutralize HIV-1 JRFL, a virus isolate resistant to most V3 antibodies.
26109728	12	64	theme	HIV	1838:1840	arg1	epidemics					1842:1850	the HIV epidemics	1834:1850	the HIV epidemics that continue to afflict millions of people worldwide	1834:1904	IMPORTANCE HIV/AIDS vaccines are crucial for controlling the HIV epidemics that continue to afflict millions of people worldwide.
26109728	10	65	from	apex	1524:1527	arg1	glycans					1553:1559	nearby glycans	1546:1559	nearby glycans	1546:1559	The 2424 epitope is focused on the very apex of V3, away from nearby glycans, facilitating antibody access.
26109728	14	66	theme	targets	2181:2187	arg1	targets					2181:2187	the few immunogenic targets	2161:2187	the few immunogenic targets on the virus envelope glycoprotein that can induce neutralizing antibodies	2161:2262	The V3 loop is one of the few immunogenic targets on the virus envelope glycoprotein that can induce neutralizing antibodies, but in many viruses, parts of V3 are inaccessible for antibody recognition.
26109728	14	66	theme	targets	2181:2187	arg1	one					2154:2156	one	2154:2156	one	2154:2156	The V3 loop is one of the few immunogenic targets on the virus envelope glycoprotein that can induce neutralizing antibodies, but in many viruses, parts of V3 are inaccessible for antibody recognition.
26109728	14	66	theme	targets	2181:2187	arg1	loop					2146:2149	The V3 loop	2139:2149	The V3 loop	2139:2149	The V3 loop is one of the few immunogenic targets on the virus envelope glycoprotein that can induce neutralizing antibodies, but in many viruses, parts of V3 are inaccessible for antibody recognition.
26109728	10	67	theme	antibody	1575:1582	arg1	access					1584:1589	antibody access	1575:1589	antibody access	1575:1589	The 2424 epitope is focused on the very apex of V3, away from nearby glycans, facilitating antibody access.
26109728	13	68	theme	significant	1961:1971	arg1	challenges					1984:1993	significant scientific challenges	1961:1993	significant scientific challenges	1961:1993	However, HIV vaccine development has been hampered by significant scientific challenges, one of which is the inability of HIV vaccine candidates evaluated thus far to elicit production of potent and broadly neutralizing antibodies.
26109728	8	69	theme	2424	1150:1153	arg1	Fab					1155:1157	2424 Fab	1150:1157	2424 Fab	1150:1157	To understand the distinct activity of 2424, we determined the crystal structure of 2424 Fab in complex with a JRFL V3 peptide and showed that the 2424 epitope is located at the tip of the V3 crown ((307)IHIGPGRAFYT(319)), dominated by interactions with His(P308), Pro(P313), and Arg(P315).
26109728	9	70	theme	2424	1377:1380	arg1	similar					1385:1391	similar	1385:1391	similar	1385:1391	The binding mode of 2424 is similar to that of the well-characterized MAb 447-52D, although 2424 is more side chain dependent.
26109728	9	70	theme	2424	1377:1380	arg1	mode					1369:1372	The binding mode	1357:1372	The binding mode of 2424	1357:1380	The binding mode of 2424 is similar to that of the well-characterized MAb 447-52D, although 2424 is more side chain dependent.
26109728	2	71	theme	resistant	371:379	arg1	viruses					381:387	the more resistant viruses	362:387	the more resistant viruses	362:387	Although most anti-V3 antibodies neutralize only the sensitive tier 1 viruses, anti-V3 antibodies effective against the more resistant viruses exist, and a better understanding of these antibodies and their epitopes would be beneficial for the development of novel vaccine immunogens against HIV.
26109728	0	72	with	Antibody	75:82	arg1	Activity					107:114	Neutralizing Activity	94:114	Neutralizing Activity against HIV-1 JRFL	94:133	Functional and Structural Characterization of Human V3-Specific Monoclonal Antibody 2424 with Neutralizing Activity against HIV-1 JRFL.
26109728	2	73	theme	tier	309:312	arg1	viruses					316:322	only the sensitive tier 1 viruses	290:322	only the sensitive tier 1 viruses	290:322	Although most anti-V3 antibodies neutralize only the sensitive tier 1 viruses, anti-V3 antibodies effective against the more resistant viruses exist, and a better understanding of these antibodies and their epitopes would be beneficial for the development of novel vaccine immunogens against HIV.
26109728	15	74	theme	virus	2440:2444	arg1	isolate					2446:2452	a virus isolate	2438:2452	a virus isolate resistant to most V3 antibodies	2438:2484	This study examined a V3-specific monoclonal antibody that can completely neutralize HIV-1 JRFL, a virus isolate resistant to most V3 antibodies.
26109728	15	74	theme	virus	2440:2444	arg1	HIV-1					2426:2430	HIV-1 JRFL, a virus isolate resistant to most V3 antibodies	2426:2484	HIV-1	2426:2430	This study examined a V3-specific monoclonal antibody that can completely neutralize HIV-1 JRFL, a virus isolate resistant to most V3 antibodies.
26109728	14	75	from	targets	2181:2187	arg1	glycoprotein					2211:2222	the virus envelope glycoprotein	2192:2222	the virus envelope glycoprotein that can induce neutralizing antibodies	2192:2262	The V3 loop is one of the few immunogenic targets on the virus envelope glycoprotein that can induce neutralizing antibodies, but in many viruses, parts of V3 are inaccessible for antibody recognition.
26109728	4	76	theme	V3	662:663	arg1	2424					669:672	the V3 MAb 2424	658:672	the V3 MAb 2424	658:672	However, the V3 MAb 2424 achieves 100% neutralization against JRFL.
26109728	2	77	theme	anti-V3	325:331	arg1	antibodies					333:342	anti-V3 antibodies	325:342	anti-V3 antibodies effective against the more resistant viruses	325:387	Although most anti-V3 antibodies neutralize only the sensitive tier 1 viruses, anti-V3 antibodies effective against the more resistant viruses exist, and a better understanding of these antibodies and their epitopes would be beneficial for the development of novel vaccine immunogens against HIV.
26109728	8	78	theme	JRFL	1177:1180	arg1	peptide					1185:1191	a JRFL V3 peptide	1175:1191	a JRFL V3 peptide	1175:1191	To understand the distinct activity of 2424, we determined the crystal structure of 2424 Fab in complex with a JRFL V3 peptide and showed that the 2424 epitope is located at the tip of the V3 crown ((307)IHIGPGRAFYT(319)), dominated by interactions with His(P308), Pro(P313), and Arg(P315).
26109728	16	79	theme	V3	2603:2604	arg1	parts					2594:2598	other parts	2588:2598	other parts of V3	2588:2604	Our data reveal that this antibody recognizes the most distal tip of V3, which is not as occluded as other parts of V3.
26109728	8	80	with	interactions	1302:1313	arg1	His					1320:1322	His(P308)	1320:1328	His(P308)	1320:1328	To understand the distinct activity of 2424, we determined the crystal structure of 2424 Fab in complex with a JRFL V3 peptide and showed that the 2424 epitope is located at the tip of the V3 crown ((307)IHIGPGRAFYT(319)), dominated by interactions with His(P308), Pro(P313), and Arg(P315).
26109728	8	80	with	interactions	1302:1313	arg1	Arg					1346:1348	Arg(P315)	1346:1354	Arg(P315)	1346:1354	To understand the distinct activity of 2424, we determined the crystal structure of 2424 Fab in complex with a JRFL V3 peptide and showed that the 2424 epitope is located at the tip of the V3 crown ((307)IHIGPGRAFYT(319)), dominated by interactions with His(P308), Pro(P313), and Arg(P315).
26109728	8	80	with	interactions	1302:1313	arg1	Pro					1331:1333	Pro(P313)	1331:1339	Pro(P313)	1331:1339	To understand the distinct activity of 2424, we determined the crystal structure of 2424 Fab in complex with a JRFL V3 peptide and showed that the 2424 epitope is located at the tip of the V3 crown ((307)IHIGPGRAFYT(319)), dominated by interactions with His(P308), Pro(P313), and Arg(P315).
26109728	7	81	theme	envelope	1056:1063	arg1	composition					1031:1041	the glycan composition	1020:1041	the glycan composition of the virus envelope	1020:1063	Studies of 2424-mediated neutralization of JRFL produced with a mannosidase inhibitor further revealed that its neutralizing activity is unaffected by the glycan composition of the virus envelope.
26109728	3	82	theme	V3-specific	607:617	arg1	MAbs					642:645	MAbs	642:645	MAbs	642:645	The HIV-1 isolate JRFL with its cryptic V3 is resistant to most V3-specific monoclonal antibodies (MAbs).
26109728	3	82	theme	V3-specific	607:617	arg1	antibodies					630:639	monoclonal antibodies	619:639	most V3-specific monoclonal antibodies (MAbs)	602:646	The HIV-1 isolate JRFL with its cryptic V3 is resistant to most V3-specific monoclonal antibodies (MAbs).
26109728	13	83	theme	neutralizing	2114:2125	arg1	antibodies					2127:2136	potent and broadly neutralizing antibodies	2095:2136	potent and broadly neutralizing antibodies	2095:2136	However, HIV vaccine development has been hampered by significant scientific challenges, one of which is the inability of HIV vaccine candidates evaluated thus far to elicit production of potent and broadly neutralizing antibodies.
26109728	8	84	theme	V3	1182:1183	arg1	peptide					1185:1191	a JRFL V3 peptide	1175:1191	a JRFL V3 peptide	1175:1191	To understand the distinct activity of 2424, we determined the crystal structure of 2424 Fab in complex with a JRFL V3 peptide and showed that the 2424 epitope is located at the tip of the V3 crown ((307)IHIGPGRAFYT(319)), dominated by interactions with His(P308), Pro(P313), and Arg(P315).
26109728	11	85	theme	V3	1651:1652	arg1	epitopes					1660:1667	the other V3 crown epitopes	1641:1667	the other V3 crown epitopes	1641:1667	This feature distinguishes the 2424 epitope from the other V3 crown epitopes and indicates that the tip of V3 is a potential site to target and incorporate into HIV vaccine immunogens.
26109728	7	86	theme	glycan	1024:1029	arg1	composition					1031:1041	the glycan composition	1020:1041	the glycan composition of the virus envelope	1020:1063	Studies of 2424-mediated neutralization of JRFL produced with a mannosidase inhibitor further revealed that its neutralizing activity is unaffected by the glycan composition of the virus envelope.
26109728	3	87	dep	isolate	553:559	arg1	JRFL					561:564	JRFL	561:564	JRFL	561:564	The HIV-1 isolate JRFL with its cryptic V3 is resistant to most V3-specific monoclonal antibodies (MAbs).
26109728	1	88	theme	The	147:149	arg1	region					154:159	UNLABELLED The V3 region	136:159	UNLABELLED The V3 region of HIV-1 gp120	136:174	UNLABELLED The V3 region of HIV-1 gp120 is important for virus-coreceptor interaction and highly immunogenic.
26109728	3	89	theme	cryptic	575:581	arg1	V3					583:584	its cryptic V3	571:584	its cryptic V3	571:584	The HIV-1 isolate JRFL with its cryptic V3 is resistant to most V3-specific monoclonal antibodies (MAbs).
26109728	8	90	theme	V3	1255:1256	arg1	IHIGPGRAFYT					1270:1280	(307)IHIGPGRAFYT(319)	1265:1285	(307)IHIGPGRAFYT(319)	1265:1285	To understand the distinct activity of 2424, we determined the crystal structure of 2424 Fab in complex with a JRFL V3 peptide and showed that the 2424 epitope is located at the tip of the V3 crown ((307)IHIGPGRAFYT(319)), dominated by interactions with His(P308), Pro(P313), and Arg(P315).
26109728	8	90	theme	V3	1255:1256	arg1	crown					1258:1262	the V3 crown	1251:1262	the V3 crown ((307)IHIGPGRAFYT(319))	1251:1286	To understand the distinct activity of 2424, we determined the crystal structure of 2424 Fab in complex with a JRFL V3 peptide and showed that the 2424 epitope is located at the tip of the V3 crown ((307)IHIGPGRAFYT(319)), dominated by interactions with His(P308), Pro(P313), and Arg(P315).
26109728	5	91	theme	IGLV2-28	749:756	arg1	genes					758:762	IGLV2-28 genes	749:762	IGLV2-28 genes	749:762	2424 is encoded by IGHV3-53 and IGLV2-28 genes, a pairing rarely used by the other V3 MAbs.
26109728	13	92	theme	HIV	1916:1918	arg1	development					1928:1938	HIV vaccine development	1916:1938	HIV vaccine development	1916:1938	However, HIV vaccine development has been hampered by significant scientific challenges, one of which is the inability of HIV vaccine candidates evaluated thus far to elicit production of potent and broadly neutralizing antibodies.
26109728	17	93	theme	HIV	2716:2718	arg1	immunogens					2728:2737	HIV vaccine immunogens	2716:2737	HIV vaccine immunogens	2716:2737	Hence, the epitope of 2424 is in one of the vulnerable sites on the virus that may be exploited in designing HIV vaccine immunogens.
26109728	2	94	theme	immunogens	519:528	arg1	development					490:500	the development	486:500	the development of novel vaccine immunogens against HIV	486:540	Although most anti-V3 antibodies neutralize only the sensitive tier 1 viruses, anti-V3 antibodies effective against the more resistant viruses exist, and a better understanding of these antibodies and their epitopes would be beneficial for the development of novel vaccine immunogens against HIV.
26109728	12	95	theme	IMPORTANCE	1777:1786	arg1	vaccines					1797:1804	IMPORTANCE HIV/AIDS vaccines	1777:1804	IMPORTANCE HIV/AIDS vaccines	1777:1804	IMPORTANCE HIV/AIDS vaccines are crucial for controlling the HIV epidemics that continue to afflict millions of people worldwide.
26109728	4	96	theme	100	683:685	arg1	%					686:686	%	686:686	%	686:686	However, the V3 MAb 2424 achieves 100% neutralization against JRFL.
26109728	0	97	theme	Neutralizing	94:105	arg1	Activity					107:114	Neutralizing Activity	94:114	Neutralizing Activity against HIV-1 JRFL	94:133	Functional and Structural Characterization of Human V3-Specific Monoclonal Antibody 2424 with Neutralizing Activity against HIV-1 JRFL.
26109728	7	98	theme	neutralizing	981:992	arg1	activity					994:1001	its neutralizing activity	977:1001	its neutralizing activity	977:1001	Studies of 2424-mediated neutralization of JRFL produced with a mannosidase inhibitor further revealed that its neutralizing activity is unaffected by the glycan composition of the virus envelope.
26109728	1	99	theme	HIV-1	164:168	arg1	gp120					170:174	HIV-1 gp120	164:174	HIV-1 gp120	164:174	UNLABELLED The V3 region of HIV-1 gp120 is important for virus-coreceptor interaction and highly immunogenic.
26109728	2	100	theme	novel	505:509	arg1	immunogens					519:528	novel vaccine immunogens	505:528	novel vaccine immunogens against HIV	505:540	Although most anti-V3 antibodies neutralize only the sensitive tier 1 viruses, anti-V3 antibodies effective against the more resistant viruses exist, and a better understanding of these antibodies and their epitopes would be beneficial for the development of novel vaccine immunogens against HIV.
26109728	14	101	theme	V3	2295:2296	arg1	parts					2286:2290	parts	2286:2290	parts of V3	2286:2296	The V3 loop is one of the few immunogenic targets on the virus envelope glycoprotein that can induce neutralizing antibodies, but in many viruses, parts of V3 are inaccessible for antibody recognition.
26109728	6	102	contain	has	819:821	arg2	profiles					859:866	distinct binding and neutralization profiles	823:866	profiles	859:866	2424 also has distinct binding and neutralization profiles.
26109728	6	102	contain	has	819:821	arg2	binding					832:838	distinct binding and neutralization profiles	823:866	binding	832:838	2424 also has distinct binding and neutralization profiles.
26109728	6	102	contain	has	819:821	arg1	2424					809:812	2424	809:812	2424	809:812	2424 also has distinct binding and neutralization profiles.
26109728	3	103	theme	monoclonal	619:628	arg1	MAbs					642:645	MAbs	642:645	MAbs	642:645	The HIV-1 isolate JRFL with its cryptic V3 is resistant to most V3-specific monoclonal antibodies (MAbs).
26109728	3	103	theme	monoclonal	619:628	arg1	antibodies					630:639	monoclonal antibodies	619:639	most V3-specific monoclonal antibodies (MAbs)	602:646	The HIV-1 isolate JRFL with its cryptic V3 is resistant to most V3-specific monoclonal antibodies (MAbs).
26109728	8	104	located	located	1229:1235	arg2	dominated					1289:1297	dominated	1289:1297	dominated by interactions with His(P308), Pro(P313), and Arg(P315)	1289:1354	To understand the distinct activity of 2424, we determined the crystal structure of 2424 Fab in complex with a JRFL V3 peptide and showed that the 2424 epitope is located at the tip of the V3 crown ((307)IHIGPGRAFYT(319)), dominated by interactions with His(P308), Pro(P313), and Arg(P315).
26109728	8	104	located	located	1229:1235	arg2	epitope					1218:1224	the 2424 epitope	1209:1224	the 2424 epitope	1209:1224	To understand the distinct activity of 2424, we determined the crystal structure of 2424 Fab in complex with a JRFL V3 peptide and showed that the 2424 epitope is located at the tip of the V3 crown ((307)IHIGPGRAFYT(319)), dominated by interactions with His(P308), Pro(P313), and Arg(P315).
26109728	8	104	located	located	1229:1235	arg2	located					1229:1235	located	1229:1235	located	1229:1235	To understand the distinct activity of 2424, we determined the crystal structure of 2424 Fab in complex with a JRFL V3 peptide and showed that the 2424 epitope is located at the tip of the V3 crown ((307)IHIGPGRAFYT(319)), dominated by interactions with His(P308), Pro(P313), and Arg(P315).
26109728	8	104	located	located	1229:1235	arg1	tip					1244:1246	the tip	1240:1246	the tip of the V3 crown ((307)IHIGPGRAFYT(319))	1240:1286	To understand the distinct activity of 2424, we determined the crystal structure of 2424 Fab in complex with a JRFL V3 peptide and showed that the 2424 epitope is located at the tip of the V3 crown ((307)IHIGPGRAFYT(319)), dominated by interactions with His(P308), Pro(P313), and Arg(P315).
26109728	15	105	theme	V3-specific	2363:2373	arg1	antibody					2386:2393	a V3-specific monoclonal antibody	2361:2393	a V3-specific monoclonal antibody that can completely neutralize HIV-1 JRFL, a virus isolate resistant to most V3 antibodies	2361:2484	This study examined a V3-specific monoclonal antibody that can completely neutralize HIV-1 JRFL, a virus isolate resistant to most V3 antibodies.
26109728	8	106	with	complex	1162:1168	arg1	peptide					1185:1191	a JRFL V3 peptide	1175:1191	a JRFL V3 peptide	1175:1191	To understand the distinct activity of 2424, we determined the crystal structure of 2424 Fab in complex with a JRFL V3 peptide and showed that the 2424 epitope is located at the tip of the V3 crown ((307)IHIGPGRAFYT(319)), dominated by interactions with His(P308), Pro(P313), and Arg(P315).
26109728	11	107	theme	potential	1707:1715	arg1	tip					1692:1694	the tip	1688:1694	the tip of V3	1688:1700	This feature distinguishes the 2424 epitope from the other V3 crown epitopes and indicates that the tip of V3 is a potential site to target and incorporate into HIV vaccine immunogens.
26109728	11	107	theme	potential	1707:1715	arg1	site					1717:1720	a potential site	1705:1720	a potential site to target and incorporate into HIV vaccine immunogens	1705:1774	This feature distinguishes the 2424 epitope from the other V3 crown epitopes and indicates that the tip of V3 is a potential site to target and incorporate into HIV vaccine immunogens.
26109728	7	108	theme	mannosidase	933:943	arg1	inhibitor					945:953	a mannosidase inhibitor	931:953	a mannosidase inhibitor	931:953	Studies of 2424-mediated neutralization of JRFL produced with a mannosidase inhibitor further revealed that its neutralizing activity is unaffected by the glycan composition of the virus envelope.
26109728	17	109	theme	vulnerable	2651:2660	arg1	sites					2662:2666	the vulnerable sites	2647:2666	the vulnerable sites	2647:2666	Hence, the epitope of 2424 is in one of the vulnerable sites on the virus that may be exploited in designing HIV vaccine immunogens.
26109728	5	110	theme	V3	800:801	arg1	MAbs					803:806	the other V3 MAbs	790:806	the other V3 MAbs	790:806	2424 is encoded by IGHV3-53 and IGLV2-28 genes, a pairing rarely used by the other V3 MAbs.
26109728	5	111	theme	other	794:798	arg1	MAbs					803:806	the other V3 MAbs	790:806	the other V3 MAbs	790:806	2424 is encoded by IGHV3-53 and IGLV2-28 genes, a pairing rarely used by the other V3 MAbs.
26109728	8	112	theme	distinct	1084:1091	arg1	activity					1093:1100	the distinct activity	1080:1100	the distinct activity of 2424	1080:1108	To understand the distinct activity of 2424, we determined the crystal structure of 2424 Fab in complex with a JRFL V3 peptide and showed that the 2424 epitope is located at the tip of the V3 crown ((307)IHIGPGRAFYT(319)), dominated by interactions with His(P308), Pro(P313), and Arg(P315).
26109728	9	113	theme	MAb	1427:1429	arg1	447-52D					1431:1437	the well-characterized MAb 447-52D	1404:1437	the well-characterized MAb 447-52D	1404:1437	The binding mode of 2424 is similar to that of the well-characterized MAb 447-52D, although 2424 is more side chain dependent.
26109728	2	114	theme	anti-V3	260:266	arg1	antibodies					268:277	most anti-V3 antibodies	255:277	most anti-V3 antibodies	255:277	Although most anti-V3 antibodies neutralize only the sensitive tier 1 viruses, anti-V3 antibodies effective against the more resistant viruses exist, and a better understanding of these antibodies and their epitopes would be beneficial for the development of novel vaccine immunogens against HIV.
26109728	3	115	with	isolate	553:559	arg1	V3					583:584	its cryptic V3	571:584	its cryptic V3	571:584	The HIV-1 isolate JRFL with its cryptic V3 is resistant to most V3-specific monoclonal antibodies (MAbs).
26109728	13	116	theme	candidates	2041:2050	arg1	inability					2016:2024	the inability	2012:2024	the inability of HIV vaccine candidates evaluated thus far to elicit production of potent and broadly neutralizing antibodies	2012:2136	However, HIV vaccine development has been hampered by significant scientific challenges, one of which is the inability of HIV vaccine candidates evaluated thus far to elicit production of potent and broadly neutralizing antibodies.
26109728	13	116	theme	candidates	2041:2050	arg1	one					1996:1998	one	1996:1998	one	1996:1998	However, HIV vaccine development has been hampered by significant scientific challenges, one of which is the inability of HIV vaccine candidates evaluated thus far to elicit production of potent and broadly neutralizing antibodies.
26109728	13	116	theme	candidates	2041:2050	arg1	challenges					1984:1993	significant scientific challenges	1961:1993	significant scientific challenges	1961:1993	However, HIV vaccine development has been hampered by significant scientific challenges, one of which is the inability of HIV vaccine candidates evaluated thus far to elicit production of potent and broadly neutralizing antibodies.
26109728	8	117	from	structure	1137:1145	arg1	complex					1162:1168	complex	1162:1168	complex with a JRFL V3 peptide	1162:1191	To understand the distinct activity of 2424, we determined the crystal structure of 2424 Fab in complex with a JRFL V3 peptide and showed that the 2424 epitope is located at the tip of the V3 crown ((307)IHIGPGRAFYT(319)), dominated by interactions with His(P308), Pro(P313), and Arg(P315).
26109728	13	118	theme	potent	2095:2100	arg1	antibodies					2127:2136	potent and broadly neutralizing antibodies	2095:2136	potent and broadly neutralizing antibodies	2095:2136	However, HIV vaccine development has been hampered by significant scientific challenges, one of which is the inability of HIV vaccine candidates evaluated thus far to elicit production of potent and broadly neutralizing antibodies.
26109728	14	119	theme	neutralizing	2240:2251	arg1	antibodies					2253:2262	neutralizing antibodies	2240:2262	neutralizing antibodies	2240:2262	The V3 loop is one of the few immunogenic targets on the virus envelope glycoprotein that can induce neutralizing antibodies, but in many viruses, parts of V3 are inaccessible for antibody recognition.
26109728	0	120	theme	Monoclonal	64:73	arg1	Antibody					75:82	Human V3-Specific Monoclonal Antibody 2424	46:87	Human V3-Specific Monoclonal Antibody 2424 with Neutralizing Activity against HIV-1 JRFL	46:133	Functional and Structural Characterization of Human V3-Specific Monoclonal Antibody 2424 with Neutralizing Activity against HIV-1 JRFL.
26109728	6	121	theme	distinct	823:830	arg1	binding					832:838	distinct binding and neutralization profiles	823:866	binding	832:838	2424 also has distinct binding and neutralization profiles.
26109728	15	122	theme	monoclonal	2375:2384	arg1	antibody					2386:2393	a V3-specific monoclonal antibody	2361:2393	a V3-specific monoclonal antibody that can completely neutralize HIV-1 JRFL, a virus isolate resistant to most V3 antibodies	2361:2484	This study examined a V3-specific monoclonal antibody that can completely neutralize HIV-1 JRFL, a virus isolate resistant to most V3 antibodies.
26109728	11	123	dep	site	1717:1720	arg1	incorporate					1736:1746	incorporate	1736:1746	incorporate into HIV vaccine immunogens	1736:1774	This feature distinguishes the 2424 epitope from the other V3 crown epitopes and indicates that the tip of V3 is a potential site to target and incorporate into HIV vaccine immunogens.
26109728	11	123	dep	site	1717:1720	arg1	target					1725:1730	target	1725:1730	target	1725:1730	This feature distinguishes the 2424 epitope from the other V3 crown epitopes and indicates that the tip of V3 is a potential site to target and incorporate into HIV vaccine immunogens.
26109728	2	124	theme	most	255:258	arg1	antibodies					268:277	most anti-V3 antibodies	255:277	most anti-V3 antibodies	255:277	Although most anti-V3 antibodies neutralize only the sensitive tier 1 viruses, anti-V3 antibodies effective against the more resistant viruses exist, and a better understanding of these antibodies and their epitopes would be beneficial for the development of novel vaccine immunogens against HIV.
26109728	17	125	theme	2424	2629:2632	arg1	epitope					2618:2624	the epitope	2614:2624	the epitope of 2424	2614:2632	Hence, the epitope of 2424 is in one of the vulnerable sites on the virus that may be exploited in designing HIV vaccine immunogens.
26109728	7	126	theme	2424-mediated	880:892	arg1	neutralization					894:907	2424-mediated neutralization	880:907	2424-mediated neutralization of JRFL produced with a mannosidase inhibitor	880:953	Studies of 2424-mediated neutralization of JRFL produced with a mannosidase inhibitor further revealed that its neutralizing activity is unaffected by the glycan composition of the virus envelope.
26109728	8	127	theme	crystal	1129:1135	arg1	structure					1137:1145	the crystal structure	1125:1145	the crystal structure of 2424 Fab in complex with a JRFL V3 peptide	1125:1191	To understand the distinct activity of 2424, we determined the crystal structure of 2424 Fab in complex with a JRFL V3 peptide and showed that the 2424 epitope is located at the tip of the V3 crown ((307)IHIGPGRAFYT(319)), dominated by interactions with His(P308), Pro(P313), and Arg(P315).
26109728	14	128	theme	virus	2196:2200	arg1	glycoprotein					2211:2222	the virus envelope glycoprotein	2192:2222	the virus envelope glycoprotein that can induce neutralizing antibodies	2192:2262	The V3 loop is one of the few immunogenic targets on the virus envelope glycoprotein that can induce neutralizing antibodies, but in many viruses, parts of V3 are inaccessible for antibody recognition.
26109728	3	129	theme	HIV-1	547:551	arg1	isolate					553:559	The HIV-1 isolate JRFL	543:564	The HIV-1 isolate JRFL with its cryptic V3	543:584	The HIV-1 isolate JRFL with its cryptic V3 is resistant to most V3-specific monoclonal antibodies (MAbs).
26109728	3	129	theme	HIV-1	547:551	arg1	resistant					589:597	resistant	589:597	resistant	589:597	The HIV-1 isolate JRFL with its cryptic V3 is resistant to most V3-specific monoclonal antibodies (MAbs).
24976483	0	0	theme	different	112:120	arg1	environments					125:136	different pH environments	112:136	different pH environments	112:136	Mechanically adaptive and shape-memory behaviour of chitosan-modified cellulose whisker/elastomer composites in different pH environments.
24976483	3	1	theme	modulus	794:800	arg1	stimulus					842:849	the stimulus	838:849	the stimulus	838:849	The results show that surface modification significantly enhances the modulus contrast under wet and dry conditions with the acidic solution as the stimulus, while maintaining the high modulus contrast with the basic solution as the stimulus.
24976483	3	1	theme	modulus	794:800	arg1	contrast					802:809	the high modulus contrast	785:809	the high modulus contrast with the basic solution	785:833	The results show that surface modification significantly enhances the modulus contrast under wet and dry conditions with the acidic solution as the stimulus, while maintaining the high modulus contrast with the basic solution as the stimulus.
24976483	4	2	theme	pristine	969:976	arg1	composites					985:994	those pristine CW/TPU composites	963:994	those pristine CW/TPU composites	963:994	CS-CW/TPU composites also exhibit excellent shape-memory effects in all three solutions that are comparable to those pristine CW/TPU composites.
24976483	2	3	theme	representative	560:573	arg1	solutions					575:583	three representative solutions	554:583	three representative solutions with various pH values	554:606	The effect of surface modification on the mechanically adaptive behaviour of CS-CW/TPU composites is investigated by using three representative solutions with various pH values.
24976483	2	4	with	solutions	575:583	arg1	values					601:606	various pH values	590:606	various pH values	590:606	The effect of surface modification on the mechanically adaptive behaviour of CS-CW/TPU composites is investigated by using three representative solutions with various pH values.
24976483	1	5	theme	shape-memory	213:224	arg1	behaviour					226:234	water-active mechanically adaptive and shape-memory behaviour	174:234	water-active mechanically adaptive and shape-memory behaviour in different pH environments	174:263	Biomimetic polymer composites with water-active mechanically adaptive and shape-memory behaviour in different pH environments are synthesised by using chitosan-modified cellulose whiskers (CS-CWs) as the stimulus-responsive phase and thermoplastic polyurethane (TPU) as the resilient matrix.
24976483	1	6	theme	resilient	413:421	arg1	whiskers					318:325	chitosan-modified cellulose whiskers	290:325	chitosan-modified cellulose whiskers (CS-CWs)	290:334	Biomimetic polymer composites with water-active mechanically adaptive and shape-memory behaviour in different pH environments are synthesised by using chitosan-modified cellulose whiskers (CS-CWs) as the stimulus-responsive phase and thermoplastic polyurethane (TPU) as the resilient matrix.
24976483	1	6	theme	resilient	413:421	arg1	matrix					423:428	the resilient matrix	409:428	the resilient matrix	409:428	Biomimetic polymer composites with water-active mechanically adaptive and shape-memory behaviour in different pH environments are synthesised by using chitosan-modified cellulose whiskers (CS-CWs) as the stimulus-responsive phase and thermoplastic polyurethane (TPU) as the resilient matrix.
24976483	5	7	from	activation	1010:1019	arg1	composites					1068:1077	the stretched CS-CW/TPU composites	1044:1077	the stretched CS-CW/TPU composites	1044:1077	Furthermore, activation of force generation in the stretched CS-CW/TPU composites by water absorption/desorption was observed.
24976483	3	8	with	conditions	714:723	arg1	solution					741:748	the acidic solution	730:748	the acidic solution	730:748	The results show that surface modification significantly enhances the modulus contrast under wet and dry conditions with the acidic solution as the stimulus, while maintaining the high modulus contrast with the basic solution as the stimulus.
24976483	0	9	theme	pH	122:123	arg1	environments					125:136	different pH environments	112:136	different pH environments	112:136	Mechanically adaptive and shape-memory behaviour of chitosan-modified cellulose whisker/elastomer composites in different pH environments.
24976483	4	10	theme	CW/TPU	978:983	arg1	composites					985:994	those pristine CW/TPU composites	963:994	those pristine CW/TPU composites	963:994	CS-CW/TPU composites also exhibit excellent shape-memory effects in all three solutions that are comparable to those pristine CW/TPU composites.
24976483	3	11	theme	surface	631:637	arg1	modification					639:650	surface modification	631:650	surface modification	631:650	The results show that surface modification significantly enhances the modulus contrast under wet and dry conditions with the acidic solution as the stimulus, while maintaining the high modulus contrast with the basic solution as the stimulus.
24976483	3	12	theme	basic	820:824	arg1	solution					826:833	the basic solution	816:833	the basic solution	816:833	The results show that surface modification significantly enhances the modulus contrast under wet and dry conditions with the acidic solution as the stimulus, while maintaining the high modulus contrast with the basic solution as the stimulus.
24976483	2	13	theme	surface	445:451	arg1	modification					453:464	surface modification	445:464	surface modification	445:464	The effect of surface modification on the mechanically adaptive behaviour of CS-CW/TPU composites is investigated by using three representative solutions with various pH values.
24976483	2	14	theme	pH	598:599	arg1	values					601:606	various pH values	590:606	various pH values	590:606	The effect of surface modification on the mechanically adaptive behaviour of CS-CW/TPU composites is investigated by using three representative solutions with various pH values.
24976483	2	15	theme	composites	518:527	arg1	behaviour					495:503	the mechanically adaptive behaviour	469:503	the mechanically adaptive behaviour of CS-CW/TPU composites	469:527	The effect of surface modification on the mechanically adaptive behaviour of CS-CW/TPU composites is investigated by using three representative solutions with various pH values.
24976483	2	16	theme	modification	453:464	arg1	effect					435:440	The effect	431:440	The effect of surface modification on the mechanically adaptive behaviour of CS-CW/TPU composites	431:527	The effect of surface modification on the mechanically adaptive behaviour of CS-CW/TPU composites is investigated by using three representative solutions with various pH values.
24976483	0	17	theme	adaptive	13:20	arg1	behaviour					39:47	Mechanically adaptive and shape-memory behaviour	0:47	Mechanically adaptive and shape-memory behaviour of chitosan-modified cellulose whisker/elastomer composites in different pH environments.	0:137	Mechanically adaptive and shape-memory behaviour of chitosan-modified cellulose whisker/elastomer composites in different pH environments.
24976483	0	18	from	behaviour	39:47	arg1	environments					125:136	different pH environments	112:136	different pH environments	112:136	Mechanically adaptive and shape-memory behaviour of chitosan-modified cellulose whisker/elastomer composites in different pH environments.
24976483	2	19	theme	various	590:596	arg1	values					601:606	various pH values	590:606	various pH values	590:606	The effect of surface modification on the mechanically adaptive behaviour of CS-CW/TPU composites is investigated by using three representative solutions with various pH values.
24976483	1	20	theme	different	239:247	arg1	environments					252:263	different pH environments	239:263	different pH environments	239:263	Biomimetic polymer composites with water-active mechanically adaptive and shape-memory behaviour in different pH environments are synthesised by using chitosan-modified cellulose whiskers (CS-CWs) as the stimulus-responsive phase and thermoplastic polyurethane (TPU) as the resilient matrix.
24976483	0	21	theme	Mechanically	0:11	arg1	behaviour					39:47	Mechanically adaptive and shape-memory behaviour	0:47	Mechanically adaptive and shape-memory behaviour of chitosan-modified cellulose whisker/elastomer composites in different pH environments.	0:137	Mechanically adaptive and shape-memory behaviour of chitosan-modified cellulose whisker/elastomer composites in different pH environments.
24976483	1	22	with	composites	158:167	arg1	behaviour					226:234	water-active mechanically adaptive and shape-memory behaviour	174:234	water-active mechanically adaptive and shape-memory behaviour in different pH environments	174:263	Biomimetic polymer composites with water-active mechanically adaptive and shape-memory behaviour in different pH environments are synthesised by using chitosan-modified cellulose whiskers (CS-CWs) as the stimulus-responsive phase and thermoplastic polyurethane (TPU) as the resilient matrix.
24976483	2	23	from	effect	435:440	arg1	behaviour					495:503	the mechanically adaptive behaviour	469:503	the mechanically adaptive behaviour of CS-CW/TPU composites	469:527	The effect of surface modification on the mechanically adaptive behaviour of CS-CW/TPU composites is investigated by using three representative solutions with various pH values.
24976483	5	24	theme	stretched	1048:1056	arg1	composites					1068:1077	the stretched CS-CW/TPU composites	1044:1077	the stretched CS-CW/TPU composites	1044:1077	Furthermore, activation of force generation in the stretched CS-CW/TPU composites by water absorption/desorption was observed.
24976483	1	25	theme	pH	249:250	arg1	environments					252:263	different pH environments	239:263	different pH environments	239:263	Biomimetic polymer composites with water-active mechanically adaptive and shape-memory behaviour in different pH environments are synthesised by using chitosan-modified cellulose whiskers (CS-CWs) as the stimulus-responsive phase and thermoplastic polyurethane (TPU) as the resilient matrix.
24976483	0	26	theme	shape-memory	26:37	arg1	behaviour					39:47	Mechanically adaptive and shape-memory behaviour	0:47	Mechanically adaptive and shape-memory behaviour of chitosan-modified cellulose whisker/elastomer composites in different pH environments.	0:137	Mechanically adaptive and shape-memory behaviour of chitosan-modified cellulose whisker/elastomer composites in different pH environments.
24976483	1	27	theme	stimulus-responsive	343:361	arg1	phase					363:367	the stimulus-responsive phase	339:367	the stimulus-responsive phase	339:367	Biomimetic polymer composites with water-active mechanically adaptive and shape-memory behaviour in different pH environments are synthesised by using chitosan-modified cellulose whiskers (CS-CWs) as the stimulus-responsive phase and thermoplastic polyurethane (TPU) as the resilient matrix.
24976483	1	27	theme	stimulus-responsive	343:361	arg1	whiskers					318:325	chitosan-modified cellulose whiskers	290:325	chitosan-modified cellulose whiskers (CS-CWs)	290:334	Biomimetic polymer composites with water-active mechanically adaptive and shape-memory behaviour in different pH environments are synthesised by using chitosan-modified cellulose whiskers (CS-CWs) as the stimulus-responsive phase and thermoplastic polyurethane (TPU) as the resilient matrix.
24976483	5	28	theme	water	1082:1086	arg1	absorption/desorption					1088:1108	water absorption/desorption	1082:1108	water absorption/desorption	1082:1108	Furthermore, activation of force generation in the stretched CS-CW/TPU composites by water absorption/desorption was observed.
24976483	1	29	theme	Biomimetic	139:148	arg1	composites					158:167	Biomimetic polymer composites	139:167	Biomimetic polymer composites with water-active mechanically adaptive and shape-memory behaviour in different pH environments	139:263	Biomimetic polymer composites with water-active mechanically adaptive and shape-memory behaviour in different pH environments are synthesised by using chitosan-modified cellulose whiskers (CS-CWs) as the stimulus-responsive phase and thermoplastic polyurethane (TPU) as the resilient matrix.
24976483	2	30	theme	adaptive	486:493	arg1	behaviour					495:503	the mechanically adaptive behaviour	469:503	the mechanically adaptive behaviour of CS-CW/TPU composites	469:527	The effect of surface modification on the mechanically adaptive behaviour of CS-CW/TPU composites is investigated by using three representative solutions with various pH values.
24976483	1	31	theme	thermoplastic	373:385	arg1	polyurethane					387:398	thermoplastic polyurethane	373:398	thermoplastic polyurethane (TPU)	373:404	Biomimetic polymer composites with water-active mechanically adaptive and shape-memory behaviour in different pH environments are synthesised by using chitosan-modified cellulose whiskers (CS-CWs) as the stimulus-responsive phase and thermoplastic polyurethane (TPU) as the resilient matrix.
24976483	1	31	theme	thermoplastic	373:385	arg1	TPU					401:403	TPU	401:403	TPU	401:403	Biomimetic polymer composites with water-active mechanically adaptive and shape-memory behaviour in different pH environments are synthesised by using chitosan-modified cellulose whiskers (CS-CWs) as the stimulus-responsive phase and thermoplastic polyurethane (TPU) as the resilient matrix.
24976483	2	32	theme	CS-CW/TPU	508:516	arg1	composites					518:527	CS-CW/TPU composites	508:527	CS-CW/TPU composites	508:527	The effect of surface modification on the mechanically adaptive behaviour of CS-CW/TPU composites is investigated by using three representative solutions with various pH values.
24976483	0	33	theme	cellulose	70:78	arg1	composites					98:107	chitosan-modified cellulose whisker/elastomer composites	52:107	chitosan-modified cellulose whisker/elastomer composites	52:107	Mechanically adaptive and shape-memory behaviour of chitosan-modified cellulose whisker/elastomer composites in different pH environments.
24976483	3	34	theme	acidic	734:739	arg1	solution					741:748	the acidic solution	730:748	the acidic solution	730:748	The results show that surface modification significantly enhances the modulus contrast under wet and dry conditions with the acidic solution as the stimulus, while maintaining the high modulus contrast with the basic solution as the stimulus.
24976483	3	35	theme	modulus	679:685	arg1	contrast					687:694	the modulus contrast	675:694	the modulus contrast	675:694	The results show that surface modification significantly enhances the modulus contrast under wet and dry conditions with the acidic solution as the stimulus, while maintaining the high modulus contrast with the basic solution as the stimulus.
24976483	1	36	theme	polymer	150:156	arg1	composites					158:167	Biomimetic polymer composites	139:167	Biomimetic polymer composites with water-active mechanically adaptive and shape-memory behaviour in different pH environments	139:263	Biomimetic polymer composites with water-active mechanically adaptive and shape-memory behaviour in different pH environments are synthesised by using chitosan-modified cellulose whiskers (CS-CWs) as the stimulus-responsive phase and thermoplastic polyurethane (TPU) as the resilient matrix.
24976483	0	37	theme	chitosan-modified	52:68	arg1	composites					98:107	chitosan-modified cellulose whisker/elastomer composites	52:107	chitosan-modified cellulose whisker/elastomer composites	52:107	Mechanically adaptive and shape-memory behaviour of chitosan-modified cellulose whisker/elastomer composites in different pH environments.
24976483	5	38	theme	force	1024:1028	arg1	generation					1030:1039	force generation	1024:1039	force generation	1024:1039	Furthermore, activation of force generation in the stretched CS-CW/TPU composites by water absorption/desorption was observed.
24976483	3	39	with	contrast	802:809	arg1	solution					826:833	the basic solution	816:833	the basic solution	816:833	The results show that surface modification significantly enhances the modulus contrast under wet and dry conditions with the acidic solution as the stimulus, while maintaining the high modulus contrast with the basic solution as the stimulus.
24976483	4	40	theme	shape-memory	896:907	arg1	effects					909:915	excellent shape-memory effects	886:915	excellent shape-memory effects	886:915	CS-CW/TPU composites also exhibit excellent shape-memory effects in all three solutions that are comparable to those pristine CW/TPU composites.
24976483	4	41	theme	excellent	886:894	arg1	effects					909:915	excellent shape-memory effects	886:915	excellent shape-memory effects	886:915	CS-CW/TPU composites also exhibit excellent shape-memory effects in all three solutions that are comparable to those pristine CW/TPU composites.
24976483	3	42	theme	wet	702:704	arg1	conditions					714:723	wet and dry conditions	702:723	wet and dry conditions with the acidic solution as the stimulus	702:764	The results show that surface modification significantly enhances the modulus contrast under wet and dry conditions with the acidic solution as the stimulus, while maintaining the high modulus contrast with the basic solution as the stimulus.
24976483	5	43	theme	generation	1030:1039	arg1	activation					1010:1019	activation	1010:1019	activation of force generation in the stretched CS-CW/TPU composites by water absorption/desorption	1010:1108	Furthermore, activation of force generation in the stretched CS-CW/TPU composites by water absorption/desorption was observed.
24976483	5	44	theme	CS-CW/TPU	1058:1066	arg1	composites					1068:1077	the stretched CS-CW/TPU composites	1044:1077	the stretched CS-CW/TPU composites	1044:1077	Furthermore, activation of force generation in the stretched CS-CW/TPU composites by water absorption/desorption was observed.
24976483	4	45	theme	CS-CW/TPU	852:860	arg1	composites					862:871	CS-CW/TPU composites	852:871	CS-CW/TPU composites	852:871	CS-CW/TPU composites also exhibit excellent shape-memory effects in all three solutions that are comparable to those pristine CW/TPU composites.
24976483	1	46	theme	water-active	174:185	arg1	behaviour					226:234	water-active mechanically adaptive and shape-memory behaviour	174:234	water-active mechanically adaptive and shape-memory behaviour in different pH environments	174:263	Biomimetic polymer composites with water-active mechanically adaptive and shape-memory behaviour in different pH environments are synthesised by using chitosan-modified cellulose whiskers (CS-CWs) as the stimulus-responsive phase and thermoplastic polyurethane (TPU) as the resilient matrix.
24976483	1	47	theme	chitosan-modified	290:306	arg1	phase					363:367	the stimulus-responsive phase	339:367	the stimulus-responsive phase	339:367	Biomimetic polymer composites with water-active mechanically adaptive and shape-memory behaviour in different pH environments are synthesised by using chitosan-modified cellulose whiskers (CS-CWs) as the stimulus-responsive phase and thermoplastic polyurethane (TPU) as the resilient matrix.
24976483	1	47	theme	chitosan-modified	290:306	arg1	CS-CWs					328:333	CS-CWs	328:333	CS-CWs	328:333	Biomimetic polymer composites with water-active mechanically adaptive and shape-memory behaviour in different pH environments are synthesised by using chitosan-modified cellulose whiskers (CS-CWs) as the stimulus-responsive phase and thermoplastic polyurethane (TPU) as the resilient matrix.
24976483	1	47	theme	chitosan-modified	290:306	arg1	whiskers					318:325	chitosan-modified cellulose whiskers	290:325	chitosan-modified cellulose whiskers (CS-CWs)	290:334	Biomimetic polymer composites with water-active mechanically adaptive and shape-memory behaviour in different pH environments are synthesised by using chitosan-modified cellulose whiskers (CS-CWs) as the stimulus-responsive phase and thermoplastic polyurethane (TPU) as the resilient matrix.
24976483	1	47	theme	chitosan-modified	290:306	arg1	matrix					423:428	the resilient matrix	409:428	the resilient matrix	409:428	Biomimetic polymer composites with water-active mechanically adaptive and shape-memory behaviour in different pH environments are synthesised by using chitosan-modified cellulose whiskers (CS-CWs) as the stimulus-responsive phase and thermoplastic polyurethane (TPU) as the resilient matrix.
24976483	0	48	theme	composites	98:107	arg1	behaviour					39:47	Mechanically adaptive and shape-memory behaviour	0:47	Mechanically adaptive and shape-memory behaviour of chitosan-modified cellulose whisker/elastomer composites in different pH environments.	0:137	Mechanically adaptive and shape-memory behaviour of chitosan-modified cellulose whisker/elastomer composites in different pH environments.
24976483	1	49	theme	cellulose	308:316	arg1	phase					363:367	the stimulus-responsive phase	339:367	the stimulus-responsive phase	339:367	Biomimetic polymer composites with water-active mechanically adaptive and shape-memory behaviour in different pH environments are synthesised by using chitosan-modified cellulose whiskers (CS-CWs) as the stimulus-responsive phase and thermoplastic polyurethane (TPU) as the resilient matrix.
24976483	1	49	theme	cellulose	308:316	arg1	CS-CWs					328:333	CS-CWs	328:333	CS-CWs	328:333	Biomimetic polymer composites with water-active mechanically adaptive and shape-memory behaviour in different pH environments are synthesised by using chitosan-modified cellulose whiskers (CS-CWs) as the stimulus-responsive phase and thermoplastic polyurethane (TPU) as the resilient matrix.
24976483	1	49	theme	cellulose	308:316	arg1	whiskers					318:325	chitosan-modified cellulose whiskers	290:325	chitosan-modified cellulose whiskers (CS-CWs)	290:334	Biomimetic polymer composites with water-active mechanically adaptive and shape-memory behaviour in different pH environments are synthesised by using chitosan-modified cellulose whiskers (CS-CWs) as the stimulus-responsive phase and thermoplastic polyurethane (TPU) as the resilient matrix.
24976483	1	49	theme	cellulose	308:316	arg1	matrix					423:428	the resilient matrix	409:428	the resilient matrix	409:428	Biomimetic polymer composites with water-active mechanically adaptive and shape-memory behaviour in different pH environments are synthesised by using chitosan-modified cellulose whiskers (CS-CWs) as the stimulus-responsive phase and thermoplastic polyurethane (TPU) as the resilient matrix.
24976483	0	50	theme	whisker/elastomer	80:96	arg1	composites					98:107	chitosan-modified cellulose whisker/elastomer composites	52:107	chitosan-modified cellulose whisker/elastomer composites	52:107	Mechanically adaptive and shape-memory behaviour of chitosan-modified cellulose whisker/elastomer composites in different pH environments.
24976483	3	51	theme	high	789:792	arg1	stimulus					842:849	the stimulus	838:849	the stimulus	838:849	The results show that surface modification significantly enhances the modulus contrast under wet and dry conditions with the acidic solution as the stimulus, while maintaining the high modulus contrast with the basic solution as the stimulus.
24976483	3	51	theme	high	789:792	arg1	contrast					802:809	the high modulus contrast	785:809	the high modulus contrast with the basic solution	785:833	The results show that surface modification significantly enhances the modulus contrast under wet and dry conditions with the acidic solution as the stimulus, while maintaining the high modulus contrast with the basic solution as the stimulus.
24976483	3	52	theme	dry	710:712	arg1	conditions					714:723	wet and dry conditions	702:723	wet and dry conditions with the acidic solution as the stimulus	702:764	The results show that surface modification significantly enhances the modulus contrast under wet and dry conditions with the acidic solution as the stimulus, while maintaining the high modulus contrast with the basic solution as the stimulus.
24976483	1	53	from	behaviour	226:234	arg1	environments					252:263	different pH environments	239:263	different pH environments	239:263	Biomimetic polymer composites with water-active mechanically adaptive and shape-memory behaviour in different pH environments are synthesised by using chitosan-modified cellulose whiskers (CS-CWs) as the stimulus-responsive phase and thermoplastic polyurethane (TPU) as the resilient matrix.
24976483	1	54	theme	adaptive	200:207	arg1	behaviour					226:234	water-active mechanically adaptive and shape-memory behaviour	174:234	water-active mechanically adaptive and shape-memory behaviour in different pH environments	174:263	Biomimetic polymer composites with water-active mechanically adaptive and shape-memory behaviour in different pH environments are synthesised by using chitosan-modified cellulose whiskers (CS-CWs) as the stimulus-responsive phase and thermoplastic polyurethane (TPU) as the resilient matrix.
29202699	10	0	dep	showed	1475:1480	arg1	had					1562:1564	had	1562:1564	showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount	1475:1769	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	11	1	from	differences	1835:1845	arg1	sugars					1850:1855	sugars	1850:1855	sugars	1850:1855	CONCLUSION The composition analysis of H. pylori LPS, revealed differences in sugars and fatty acids composition between a mouse adapted strain SS1 and G27.
29202699	11	1	from	differences	1835:1845	arg1	acids					1867:1871	fatty acids	1861:1871	fatty acids	1861:1871	CONCLUSION The composition analysis of H. pylori LPS, revealed differences in sugars and fatty acids composition between a mouse adapted strain SS1 and G27.
29202699	11	2	dep	adapted	1901:1907	arg1	mouse					1895:1899	mouse	1895:1899	mouse	1895:1899	CONCLUSION The composition analysis of H. pylori LPS, revealed differences in sugars and fatty acids composition between a mouse adapted strain SS1 and G27.
29202699	10	3	theme	hydroxy	1587:1593	arg1	3-OH-C10:0					1610:1619	3-OH-C10:0	1610:1619	3-OH-C10:0	1610:1619	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	10	3	theme	hydroxy	1587:1593	arg1	acid					1604:1607	3 hydroxy decanoic acid	1585:1607	3 hydroxy decanoic acid (3-OH-C10:0)	1585:1620	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	1	4	theme	ulcers	249:254	arg1	bacterium					174:182	a Gram-negative, microaerophilic bacterium	141:182	bacterium	174:182	BACKGROUND Helicobacter pylori (H. pylori) is a Gram-negative, microaerophilic bacterium that is recognized as a major cause of chronic gastritis, peptic ulcers, and gastric cancer.
29202699	1	4	theme	ulcers	249:254	arg1	cause					214:218	a major cause	206:218	a major cause of chronic gastritis, peptic ulcers, and gastric cancer	206:274	BACKGROUND Helicobacter pylori (H. pylori) is a Gram-negative, microaerophilic bacterium that is recognized as a major cause of chronic gastritis, peptic ulcers, and gastric cancer.
29202699	4	5	theme	pathogen-induced	550:565	arg1	responses					585:593	pathogen-induced host inflammatory responses	550:593	pathogen-induced host inflammatory responses resulting in chronic inflammation within the gastrointestinal tract	550:661	In addition, H. pylori LPS modulates pathogen-induced host inflammatory responses resulting in chronic inflammation within the gastrointestinal tract.
29202699	10	6	theme	lipid-A	1454:1460	arg1	portion					1462:1468	lipid-A portion	1454:1468	lipid-A portion	1454:1468	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	7	7	theme	mass	1081:1084	arg1	spectrometry					1086:1097	mass spectrometry	1081:1097	mass spectrometry	1081:1097	LPS were extracted from aqueous and phenol layer of hot-phenol water extraction method and subjected for composition analysis by gas chromatography - mass spectrometry (GC-MS) to sugar and fatty acid compositions.
29202699	10	8	from	present	1730:1736	arg1	amount					1764:1769	relatively high amount	1748:1769	relatively high amount	1748:1769	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	10	8	from	present	1730:1736	arg1	G27					1741:1743	G27	1741:1743	G27	1741:1743	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	4	9	theme	gastrointestinal	640:655	arg1	tract					657:661	the gastrointestinal tract	636:661	the gastrointestinal tract	636:661	In addition, H. pylori LPS modulates pathogen-induced host inflammatory responses resulting in chronic inflammation within the gastrointestinal tract.
29202699	10	10	from	G27	1741:1743	arg1	present					1730:1736	present	1730:1736	present	1730:1736	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	6	11	theme	H.	848:849	arg1	pylori					851:856	H. pylori	848:856	H. pylori	848:856	Therefore, LPS was analyzed from two strains of H. pylori with differing potency in inducing inflammatory responses (SS1 and G27).
29202699	8	12	theme	pylori	1204:1209	arg1	strains					1190:1196	the two strains	1182:1196	the two strains of H. pylori	1182:1209	RESULTS The major difference between the two strains of H. pylori is the presence of Rhamnose, Fucose and GalNAc in the SS1 strain, which was either not found or with low abundance in the G27 strain.
29202699	10	13	theme	considerable	1482:1493	arg1	differences					1505:1515	differences	1505:1515	differences between the two strains, phenol layer of SS1	1505:1560	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	10	13	theme	considerable	1482:1493	arg1	amount					1495:1500	considerable amount	1482:1500	considerable amount of differences between the two strains, phenol layer of SS1	1482:1560	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	2	14	theme	Gram-negative	297:309	arg1	bacteria					311:318	other Gram-negative bacteria	291:318	other Gram-negative bacteria	291:318	Comparable to other Gram-negative bacteria, lipopolysaccharides (LPS) are an important cellular component of the outer membrane of H. pylori.
29202699	8	15	dep	either	1287:1292	arg1	found					1298:1302	found	1298:1302	found	1298:1302	RESULTS The major difference between the two strains of H. pylori is the presence of Rhamnose, Fucose and GalNAc in the SS1 strain, which was either not found or with low abundance in the G27 strain.
29202699	8	16	theme	major	1157:1161	arg1	presence					1218:1225	the presence	1214:1225	the presence of Rhamnose, Fucose and GalNAc in the SS1 strain, which was either not found or with low abundance in the G27 strain	1214:1342	RESULTS The major difference between the two strains of H. pylori is the presence of Rhamnose, Fucose and GalNAc in the SS1 strain, which was either not found or with low abundance in the G27 strain.
29202699	8	16	theme	major	1157:1161	arg1	difference					1163:1172	The major difference	1153:1172	The major difference between the two strains of H. pylori	1153:1209	RESULTS The major difference between the two strains of H. pylori is the presence of Rhamnose, Fucose and GalNAc in the SS1 strain, which was either not found or with low abundance in the G27 strain.
29202699	4	17	theme	chronic	608:614	arg1	inflammation					616:627	chronic inflammation	608:627	chronic inflammation	608:627	In addition, H. pylori LPS modulates pathogen-induced host inflammatory responses resulting in chronic inflammation within the gastrointestinal tract.
29202699	7	18	theme	composition	1036:1046	arg1	analysis					1048:1055	composition analysis	1036:1055	composition analysis by gas chromatography - mass spectrometry (GC-MS) to sugar and fatty acid compositions	1036:1142	LPS were extracted from aqueous and phenol layer of hot-phenol water extraction method and subjected for composition analysis by gas chromatography - mass spectrometry (GC-MS) to sugar and fatty acid compositions.
29202699	10	19	theme	dodecanoic	1636:1645	arg1	acid					1647:1650	3-hydroxy dodecanoic acid	1626:1650	3-hydroxy dodecanoic acid (3-OH-C12:0)	1626:1663	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	10	19	theme	dodecanoic	1636:1645	arg1	3-OH-C12:0					1653:1662	3-OH-C12:0	1653:1662	3-OH-C12:0	1653:1662	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	9	20	theme	high	1364:1367	arg1	Mannose					1379:1385	Mannose	1379:1385	Mannose	1379:1385	On the other hand, high amount of Mannose was present in G27 in comparison to SS1.
29202699	9	20	theme	high	1364:1367	arg1	amount					1369:1374	high amount	1364:1374	high amount of Mannose	1364:1385	On the other hand, high amount of Mannose was present in G27 in comparison to SS1.
29202699	10	21	from	amount	1764:1769	arg1	present					1730:1736	present	1730:1736	present	1730:1736	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	6	22	dep	responses	906:914	arg1	SS1					917:919	SS1	917:919	SS1	917:919	Therefore, LPS was analyzed from two strains of H. pylori with differing potency in inducing inflammatory responses (SS1 and G27).
29202699	6	22	dep	responses	906:914	arg1	responses					906:914	inflammatory responses	893:914	inflammatory responses (SS1 and G27)	893:928	Therefore, LPS was analyzed from two strains of H. pylori with differing potency in inducing inflammatory responses (SS1 and G27).
29202699	6	22	dep	responses	906:914	arg1	G27					925:927	G27	925:927	G27	925:927	Therefore, LPS was analyzed from two strains of H. pylori with differing potency in inducing inflammatory responses (SS1 and G27).
29202699	5	23	theme	LPS	707:709	arg1	compositions					711:722	the comparative LPS compositions	691:722	the comparative LPS compositions of different strains of H. pylori with varied degree of virulence in human	691:797	Very little is known about the comparative LPS compositions of different strains of H. pylori with varied degree of virulence in human.
29202699	7	24	theme	method	1011:1016	arg1	layer					974:978	aqueous and phenol layer	955:978	layer	974:978	LPS were extracted from aqueous and phenol layer of hot-phenol water extraction method and subjected for composition analysis by gas chromatography - mass spectrometry (GC-MS) to sugar and fatty acid compositions.
29202699	11	25	theme	composition	1787:1797	arg1	analysis					1799:1806	The composition analysis	1783:1806	The composition analysis of H. pylori LPS,	1783:1824	CONCLUSION The composition analysis of H. pylori LPS, revealed differences in sugars and fatty acids composition between a mouse adapted strain SS1 and G27.
29202699	11	26	theme	strain	1909:1914	arg1	SS1					1916:1918	a mouse adapted strain SS1	1893:1918	a mouse adapted strain SS1	1893:1918	CONCLUSION The composition analysis of H. pylori LPS, revealed differences in sugars and fatty acids composition between a mouse adapted strain SS1 and G27.
29202699	10	27	attach	present	1730:1736	arg1	amount					1764:1769	relatively high amount	1748:1769	relatively high amount	1748:1769	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	10	27	attach	present	1730:1736	arg2	C14:0					1719:1723	C14:0	1719:1723	C14:0	1719:1723	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	10	27	attach	present	1730:1736	arg1	G27					1741:1743	G27	1741:1743	G27	1741:1743	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	10	27	attach	present	1730:1736	arg2	acid					1713:1716	myristic acid	1704:1716	myristic acid (C14:0)	1704:1724	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	7	28	theme	water	994:998	arg1	method					1011:1016	hot-phenol water extraction method	983:1016	hot-phenol water extraction method	983:1016	LPS were extracted from aqueous and phenol layer of hot-phenol water extraction method and subjected for composition analysis by gas chromatography - mass spectrometry (GC-MS) to sugar and fatty acid compositions.
29202699	11	29	dep	sugars	1850:1855	arg1	composition					1873:1883	composition	1873:1883	composition	1873:1883	CONCLUSION The composition analysis of H. pylori LPS, revealed differences in sugars and fatty acids composition between a mouse adapted strain SS1 and G27.
29202699	3	30	theme	organism	435:442	arg1	LPS					423:425	The LPS	419:425	The LPS of this organism	419:442	The LPS of this organism plays a key role in its colonization and persistence in the stomach.
29202699	5	31	theme	H.	748:749	arg1	strains					737:743	different strains	727:743	different strains of H. pylori with varied degree of virulence in human	727:797	Very little is known about the comparative LPS compositions of different strains of H. pylori with varied degree of virulence in human.
29202699	0	32	theme	lipopolysaccharides	14:32	arg1	Comparison					0:9	Comparison	0:9	Comparison of lipopolysaccharides	0:32	Comparison of lipopolysaccharides composition of two different strains of Helicobacter pylori.
29202699	1	33	theme	Gram-negative	143:155	arg1	bacterium					174:182	a Gram-negative, microaerophilic bacterium	141:182	bacterium	174:182	BACKGROUND Helicobacter pylori (H. pylori) is a Gram-negative, microaerophilic bacterium that is recognized as a major cause of chronic gastritis, peptic ulcers, and gastric cancer.
29202699	1	33	theme	Gram-negative	143:155	arg1	pylori					119:124	BACKGROUND Helicobacter pylori	95:124	BACKGROUND Helicobacter pylori (H. pylori)	95:136	BACKGROUND Helicobacter pylori (H. pylori) is a Gram-negative, microaerophilic bacterium that is recognized as a major cause of chronic gastritis, peptic ulcers, and gastric cancer.
29202699	1	33	theme	Gram-negative	143:155	arg1	cause					214:218	a major cause	206:218	a major cause of chronic gastritis, peptic ulcers, and gastric cancer	206:274	BACKGROUND Helicobacter pylori (H. pylori) is a Gram-negative, microaerophilic bacterium that is recognized as a major cause of chronic gastritis, peptic ulcers, and gastric cancer.
29202699	8	34	theme	SS1	1265:1267	arg1	strain					1269:1274	the SS1 strain	1261:1274	the SS1 strain	1261:1274	RESULTS The major difference between the two strains of H. pylori is the presence of Rhamnose, Fucose and GalNAc in the SS1 strain, which was either not found or with low abundance in the G27 strain.
29202699	8	34	theme	SS1	1265:1267	arg1	either					1287:1292	either	1287:1292	either	1287:1292	RESULTS The major difference between the two strains of H. pylori is the presence of Rhamnose, Fucose and GalNAc in the SS1 strain, which was either not found or with low abundance in the G27 strain.
29202699	0	35	theme	pylori	87:92	arg1	strains					63:69	two different strains	49:69	two different strains of Helicobacter pylori	49:92	Comparison of lipopolysaccharides composition of two different strains of Helicobacter pylori.
29202699	1	36	theme	microaerophilic	158:172	arg1	bacterium					174:182	a Gram-negative, microaerophilic bacterium	141:182	bacterium	174:182	BACKGROUND Helicobacter pylori (H. pylori) is a Gram-negative, microaerophilic bacterium that is recognized as a major cause of chronic gastritis, peptic ulcers, and gastric cancer.
29202699	1	36	theme	microaerophilic	158:172	arg1	pylori					119:124	BACKGROUND Helicobacter pylori	95:124	BACKGROUND Helicobacter pylori (H. pylori)	95:136	BACKGROUND Helicobacter pylori (H. pylori) is a Gram-negative, microaerophilic bacterium that is recognized as a major cause of chronic gastritis, peptic ulcers, and gastric cancer.
29202699	1	36	theme	microaerophilic	158:172	arg1	cause					214:218	a major cause	206:218	a major cause of chronic gastritis, peptic ulcers, and gastric cancer	206:274	BACKGROUND Helicobacter pylori (H. pylori) is a Gram-negative, microaerophilic bacterium that is recognized as a major cause of chronic gastritis, peptic ulcers, and gastric cancer.
29202699	4	37	dep	H.	526:527	arg1	pylori					529:534	H. pylori LPS	526:538	H. pylori LPS	526:538	In addition, H. pylori LPS modulates pathogen-induced host inflammatory responses resulting in chronic inflammation within the gastrointestinal tract.
29202699	4	37	dep	H.	526:527	arg1	LPS					536:538	LPS	536:538	H. pylori LPS	526:538	In addition, H. pylori LPS modulates pathogen-induced host inflammatory responses resulting in chronic inflammation within the gastrointestinal tract.
29202699	1	38	dep	H.	127:128	arg1	pylori					130:135	H. pylori	127:135	H. pylori	127:135	BACKGROUND Helicobacter pylori (H. pylori) is a Gram-negative, microaerophilic bacterium that is recognized as a major cause of chronic gastritis, peptic ulcers, and gastric cancer.
29202699	10	39	theme	phenol	1542:1547	arg1	strains					1533:1539	the two strains	1525:1539	the two strains	1525:1539	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	10	39	theme	phenol	1542:1547	arg1	layer					1549:1553	phenol layer	1542:1553	phenol layer of SS1	1542:1560	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	5	40	theme	strains	737:743	arg1	compositions					711:722	the comparative LPS compositions	691:722	the comparative LPS compositions of different strains of H. pylori with varied degree of virulence in human	691:797	Very little is known about the comparative LPS compositions of different strains of H. pylori with varied degree of virulence in human.
29202699	1	41	theme	gastric	261:267	arg1	cancer					269:274	gastric cancer	261:274	gastric cancer	261:274	BACKGROUND Helicobacter pylori (H. pylori) is a Gram-negative, microaerophilic bacterium that is recognized as a major cause of chronic gastritis, peptic ulcers, and gastric cancer.
29202699	1	42	theme	Helicobacter	106:117	arg1	H.					127:128	H.	127:128	H.	127:128	BACKGROUND Helicobacter pylori (H. pylori) is a Gram-negative, microaerophilic bacterium that is recognized as a major cause of chronic gastritis, peptic ulcers, and gastric cancer.
29202699	1	42	theme	Helicobacter	106:117	arg1	pylori					119:124	BACKGROUND Helicobacter pylori	95:124	BACKGROUND Helicobacter pylori (H. pylori)	95:136	BACKGROUND Helicobacter pylori (H. pylori) is a Gram-negative, microaerophilic bacterium that is recognized as a major cause of chronic gastritis, peptic ulcers, and gastric cancer.
29202699	1	42	theme	Helicobacter	106:117	arg1	bacterium					174:182	a Gram-negative, microaerophilic bacterium	141:182	bacterium	174:182	BACKGROUND Helicobacter pylori (H. pylori) is a Gram-negative, microaerophilic bacterium that is recognized as a major cause of chronic gastritis, peptic ulcers, and gastric cancer.
29202699	12	43	theme	host-pathogen	2000:2012	arg1	interaction					2014:2024	host-pathogen interaction	2000:2024	host-pathogen interaction	2000:2024	This knowledge provides a novel way to dissect out their importance in host-pathogen interaction in further studies.
29202699	2	44	theme	H.	408:409	arg1	pylori					411:416	H. pylori	408:416	H. pylori	408:416	Comparable to other Gram-negative bacteria, lipopolysaccharides (LPS) are an important cellular component of the outer membrane of H. pylori.
29202699	7	45	theme	fatty	1120:1124	arg1	compositions					1131:1142	sugar and fatty acid compositions	1110:1142	compositions	1131:1142	LPS were extracted from aqueous and phenol layer of hot-phenol water extraction method and subjected for composition analysis by gas chromatography - mass spectrometry (GC-MS) to sugar and fatty acid compositions.
29202699	8	46	theme	low	1312:1314	arg1	abundance					1316:1324	low abundance	1312:1324	low abundance in the G27 strain	1312:1342	RESULTS The major difference between the two strains of H. pylori is the presence of Rhamnose, Fucose and GalNAc in the SS1 strain, which was either not found or with low abundance in the G27 strain.
29202699	2	47	theme	membrane	396:403	arg1	component					373:381	an important cellular component	351:381	an important cellular component of the outer membrane of H. pylori	351:416	Comparable to other Gram-negative bacteria, lipopolysaccharides (LPS) are an important cellular component of the outer membrane of H. pylori.
29202699	2	47	theme	membrane	396:403	arg1	lipopolysaccharides					321:339	lipopolysaccharides	321:339	lipopolysaccharides (LPS)	321:345	Comparable to other Gram-negative bacteria, lipopolysaccharides (LPS) are an important cellular component of the outer membrane of H. pylori.
29202699	7	48	theme	sugar	1110:1114	arg1	compositions					1131:1142	sugar and fatty acid compositions	1110:1142	compositions	1131:1142	LPS were extracted from aqueous and phenol layer of hot-phenol water extraction method and subjected for composition analysis by gas chromatography - mass spectrometry (GC-MS) to sugar and fatty acid compositions.
29202699	5	49	theme	virulence	780:788	arg1	degree					770:775	varied degree	763:775	varied degree of virulence in human	763:797	Very little is known about the comparative LPS compositions of different strains of H. pylori with varied degree of virulence in human.
29202699	8	50	dep	RESULTS	1145:1151	arg1	presence					1218:1225	the presence	1214:1225	the presence of Rhamnose, Fucose and GalNAc in the SS1 strain, which was either not found or with low abundance in the G27 strain	1214:1342	RESULTS The major difference between the two strains of H. pylori is the presence of Rhamnose, Fucose and GalNAc in the SS1 strain, which was either not found or with low abundance in the G27 strain.
29202699	8	50	dep	RESULTS	1145:1151	arg1	difference					1163:1172	The major difference	1153:1172	The major difference between the two strains of H. pylori	1153:1209	RESULTS The major difference between the two strains of H. pylori is the presence of Rhamnose, Fucose and GalNAc in the SS1 strain, which was either not found or with low abundance in the G27 strain.
29202699	8	51	theme	G27	1333:1335	arg1	strain					1337:1342	the G27 strain	1329:1342	the G27 strain	1329:1342	RESULTS The major difference between the two strains of H. pylori is the presence of Rhamnose, Fucose and GalNAc in the SS1 strain, which was either not found or with low abundance in the G27 strain.
29202699	10	52	located	present	1730:1736	arg1	amount					1764:1769	relatively high amount	1748:1769	relatively high amount	1748:1769	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	10	52	located	present	1730:1736	arg2	C14:0					1719:1723	C14:0	1719:1723	C14:0	1719:1723	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	10	52	located	present	1730:1736	arg1	G27					1741:1743	G27	1741:1743	G27	1741:1743	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	10	52	located	present	1730:1736	arg2	acid					1713:1716	myristic acid	1704:1716	myristic acid (C14:0)	1704:1724	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	4	53	theme	host	567:570	arg1	responses					585:593	pathogen-induced host inflammatory responses	550:593	pathogen-induced host inflammatory responses resulting in chronic inflammation within the gastrointestinal tract	550:661	In addition, H. pylori LPS modulates pathogen-induced host inflammatory responses resulting in chronic inflammation within the gastrointestinal tract.
29202699	10	54	theme	acid	1434:1437	arg1	composition					1439:1449	Fatty acid composition	1428:1449	Fatty acid composition of lipid-A portion	1428:1468	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	2	55	theme	important	354:362	arg1	component					373:381	an important cellular component	351:381	an important cellular component of the outer membrane of H. pylori	351:416	Comparable to other Gram-negative bacteria, lipopolysaccharides (LPS) are an important cellular component of the outer membrane of H. pylori.
29202699	2	55	theme	important	354:362	arg1	lipopolysaccharides					321:339	lipopolysaccharides	321:339	lipopolysaccharides (LPS)	321:345	Comparable to other Gram-negative bacteria, lipopolysaccharides (LPS) are an important cellular component of the outer membrane of H. pylori.
29202699	1	56	theme	gastritis	231:239	arg1	bacterium					174:182	a Gram-negative, microaerophilic bacterium	141:182	bacterium	174:182	BACKGROUND Helicobacter pylori (H. pylori) is a Gram-negative, microaerophilic bacterium that is recognized as a major cause of chronic gastritis, peptic ulcers, and gastric cancer.
29202699	1	56	theme	gastritis	231:239	arg1	cause					214:218	a major cause	206:218	a major cause of chronic gastritis, peptic ulcers, and gastric cancer	206:274	BACKGROUND Helicobacter pylori (H. pylori) is a Gram-negative, microaerophilic bacterium that is recognized as a major cause of chronic gastritis, peptic ulcers, and gastric cancer.
29202699	6	57	theme	inflammatory	893:904	arg1	SS1					917:919	SS1	917:919	SS1	917:919	Therefore, LPS was analyzed from two strains of H. pylori with differing potency in inducing inflammatory responses (SS1 and G27).
29202699	6	57	theme	inflammatory	893:904	arg1	responses					906:914	inflammatory responses	893:914	inflammatory responses (SS1 and G27)	893:928	Therefore, LPS was analyzed from two strains of H. pylori with differing potency in inducing inflammatory responses (SS1 and G27).
29202699	6	57	theme	inflammatory	893:904	arg1	G27					925:927	G27	925:927	G27	925:927	Therefore, LPS was analyzed from two strains of H. pylori with differing potency in inducing inflammatory responses (SS1 and G27).
29202699	11	58	dep	CONCLUSION	1772:1781	arg1	revealed					1826:1833	revealed	1826:1833	revealed differences in sugars and fatty acids composition between a mouse adapted strain SS1 and G27	1826:1926	CONCLUSION The composition analysis of H. pylori LPS, revealed differences in sugars and fatty acids composition between a mouse adapted strain SS1 and G27.
29202699	10	59	theme	decanoic	1595:1602	arg1	3-OH-C10:0					1610:1619	3-OH-C10:0	1610:1619	3-OH-C10:0	1610:1619	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	10	59	theme	decanoic	1595:1602	arg1	acid					1604:1607	3 hydroxy decanoic acid	1585:1607	3 hydroxy decanoic acid (3-OH-C10:0)	1585:1620	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	1	60	theme	peptic	242:247	arg1	ulcers					249:254	peptic ulcers	242:254	peptic ulcers	242:254	BACKGROUND Helicobacter pylori (H. pylori) is a Gram-negative, microaerophilic bacterium that is recognized as a major cause of chronic gastritis, peptic ulcers, and gastric cancer.
29202699	9	61	from	comparison	1409:1418	arg1	present					1391:1397	present	1391:1397	present	1391:1397	On the other hand, high amount of Mannose was present in G27 in comparison to SS1.
29202699	10	62	theme	portion	1462:1468	arg1	composition					1439:1449	Fatty acid composition	1428:1449	Fatty acid composition of lipid-A portion	1428:1468	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	3	63	from	colonization	468:479	arg1	stomach					504:510	the stomach	500:510	the stomach	500:510	The LPS of this organism plays a key role in its colonization and persistence in the stomach.
29202699	10	64	theme	3-hydroxy	1626:1634	arg1	acid					1647:1650	3-hydroxy dodecanoic acid	1626:1650	3-hydroxy dodecanoic acid (3-OH-C12:0)	1626:1663	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	10	64	theme	3-hydroxy	1626:1634	arg1	3-OH-C12:0					1653:1662	3-OH-C12:0	1653:1662	3-OH-C12:0	1653:1662	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	9	65	from	G27	1402:1404	arg1	present					1391:1397	present	1391:1397	present	1391:1397	On the other hand, high amount of Mannose was present in G27 in comparison to SS1.
29202699	7	66	theme	gas	1060:1062	arg1	GC-MS					1100:1104	GC-MS	1100:1104	GC-MS	1100:1104	LPS were extracted from aqueous and phenol layer of hot-phenol water extraction method and subjected for composition analysis by gas chromatography - mass spectrometry (GC-MS) to sugar and fatty acid compositions.
29202699	7	66	theme	gas	1060:1062	arg1	chromatography					1064:1077	gas chromatography	1060:1077	gas chromatography	1060:1077	LPS were extracted from aqueous and phenol layer of hot-phenol water extraction method and subjected for composition analysis by gas chromatography - mass spectrometry (GC-MS) to sugar and fatty acid compositions.
29202699	3	67	from	persistence	485:495	arg1	stomach					504:510	the stomach	500:510	the stomach	500:510	The LPS of this organism plays a key role in its colonization and persistence in the stomach.
29202699	8	68	from	abundance	1316:1324	arg1	strain					1337:1342	the G27 strain	1329:1342	the G27 strain	1329:1342	RESULTS The major difference between the two strains of H. pylori is the presence of Rhamnose, Fucose and GalNAc in the SS1 strain, which was either not found or with low abundance in the G27 strain.
29202699	0	69	theme	different	53:61	arg1	strains					63:69	two different strains	49:69	two different strains of Helicobacter pylori	49:92	Comparison of lipopolysaccharides composition of two different strains of Helicobacter pylori.
29202699	6	70	theme	pylori	851:856	arg1	strains					837:843	two strains	833:843	two strains of H. pylori	833:856	Therefore, LPS was analyzed from two strains of H. pylori with differing potency in inducing inflammatory responses (SS1 and G27).
29202699	9	71	from	present	1391:1397	arg1	comparison					1409:1418	comparison	1409:1418	comparison to SS1	1409:1425	On the other hand, high amount of Mannose was present in G27 in comparison to SS1.
29202699	9	71	from	present	1391:1397	arg1	G27					1402:1404	G27	1402:1404	G27	1402:1404	On the other hand, high amount of Mannose was present in G27 in comparison to SS1.
29202699	10	72	theme	high	1759:1762	arg1	amount					1764:1769	relatively high amount	1748:1769	relatively high amount	1748:1769	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	8	73	attach	presence	1218:1225	arg2	Rhamnose					1230:1237	Rhamnose	1230:1237	Rhamnose	1230:1237	RESULTS The major difference between the two strains of H. pylori is the presence of Rhamnose, Fucose and GalNAc in the SS1 strain, which was either not found or with low abundance in the G27 strain.
29202699	8	73	attach	presence	1218:1225	arg1	strain					1269:1274	the SS1 strain	1261:1274	the SS1 strain	1261:1274	RESULTS The major difference between the two strains of H. pylori is the presence of Rhamnose, Fucose and GalNAc in the SS1 strain, which was either not found or with low abundance in the G27 strain.
29202699	8	73	attach	presence	1218:1225	arg2	GalNAc					1251:1256	GalNAc	1251:1256	GalNAc	1251:1256	RESULTS The major difference between the two strains of H. pylori is the presence of Rhamnose, Fucose and GalNAc in the SS1 strain, which was either not found or with low abundance in the G27 strain.
29202699	8	73	attach	presence	1218:1225	arg1	either					1287:1292	either	1287:1292	either	1287:1292	RESULTS The major difference between the two strains of H. pylori is the presence of Rhamnose, Fucose and GalNAc in the SS1 strain, which was either not found or with low abundance in the G27 strain.
29202699	8	73	attach	presence	1218:1225	arg2	Fucose					1240:1245	Fucose	1240:1245	Fucose	1240:1245	RESULTS The major difference between the two strains of H. pylori is the presence of Rhamnose, Fucose and GalNAc in the SS1 strain, which was either not found or with low abundance in the G27 strain.
29202699	9	74	theme	other	1352:1356	arg1	hand					1358:1361	the other hand	1348:1361	the other hand	1348:1361	On the other hand, high amount of Mannose was present in G27 in comparison to SS1.
29202699	8	75	theme	H.	1201:1202	arg1	pylori					1204:1209	H. pylori	1201:1209	H. pylori	1201:1209	RESULTS The major difference between the two strains of H. pylori is the presence of Rhamnose, Fucose and GalNAc in the SS1 strain, which was either not found or with low abundance in the G27 strain.
29202699	10	76	attach	present	1680:1686	arg2	3-OH-C10:0					1610:1619	3-OH-C10:0	1610:1619	3-OH-C10:0	1610:1619	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	10	76	attach	present	1680:1686	arg2	acid					1647:1650	3-hydroxy dodecanoic acid	1626:1650	3-hydroxy dodecanoic acid (3-OH-C12:0)	1626:1663	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	10	76	attach	present	1680:1686	arg2	acid					1604:1607	3 hydroxy decanoic acid	1585:1607	3 hydroxy decanoic acid (3-OH-C10:0)	1585:1620	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	10	76	attach	present	1680:1686	arg1	G27					1691:1693	G27	1691:1693	G27	1691:1693	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	10	76	attach	present	1680:1686	arg2	3-OH-C12:0					1653:1662	3-OH-C12:0	1653:1662	3-OH-C12:0	1653:1662	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	10	76	attach	present	1680:1686	arg2	amount					1575:1580	enhanced amount	1566:1580	enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount	1566:1769	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	2	77	theme	other	291:295	arg1	bacteria					311:318	other Gram-negative bacteria	291:318	other Gram-negative bacteria	291:318	Comparable to other Gram-negative bacteria, lipopolysaccharides (LPS) are an important cellular component of the outer membrane of H. pylori.
29202699	11	78	dep	H.	1811:1812	arg1	pylori					1814:1819	H. pylori LPS,	1811:1824	pylori	1814:1819	CONCLUSION The composition analysis of H. pylori LPS, revealed differences in sugars and fatty acids composition between a mouse adapted strain SS1 and G27.
29202699	11	78	dep	H.	1811:1812	arg1	LPS					1821:1823	LPS	1821:1823	LPS	1821:1823	CONCLUSION The composition analysis of H. pylori LPS, revealed differences in sugars and fatty acids composition between a mouse adapted strain SS1 and G27.
29202699	11	79	theme	adapted	1901:1907	arg1	SS1					1916:1918	a mouse adapted strain SS1	1893:1918	a mouse adapted strain SS1	1893:1918	CONCLUSION The composition analysis of H. pylori LPS, revealed differences in sugars and fatty acids composition between a mouse adapted strain SS1 and G27.
29202699	5	80	theme	comparative	695:705	arg1	compositions					711:722	the comparative LPS compositions	691:722	the comparative LPS compositions of different strains of H. pylori with varied degree of virulence in human	691:797	Very little is known about the comparative LPS compositions of different strains of H. pylori with varied degree of virulence in human.
29202699	1	81	theme	major	208:212	arg1	bacterium					174:182	a Gram-negative, microaerophilic bacterium	141:182	bacterium	174:182	BACKGROUND Helicobacter pylori (H. pylori) is a Gram-negative, microaerophilic bacterium that is recognized as a major cause of chronic gastritis, peptic ulcers, and gastric cancer.
29202699	1	81	theme	major	208:212	arg1	cause					214:218	a major cause	206:218	a major cause of chronic gastritis, peptic ulcers, and gastric cancer	206:274	BACKGROUND Helicobacter pylori (H. pylori) is a Gram-negative, microaerophilic bacterium that is recognized as a major cause of chronic gastritis, peptic ulcers, and gastric cancer.
29202699	9	82	located	present	1391:1397	arg2	Mannose					1379:1385	Mannose	1379:1385	Mannose	1379:1385	On the other hand, high amount of Mannose was present in G27 in comparison to SS1.
29202699	9	82	located	present	1391:1397	arg1	hand					1358:1361	the other hand	1348:1361	the other hand	1348:1361	On the other hand, high amount of Mannose was present in G27 in comparison to SS1.
29202699	9	82	located	present	1391:1397	arg1	comparison					1409:1418	comparison	1409:1418	comparison to SS1	1409:1425	On the other hand, high amount of Mannose was present in G27 in comparison to SS1.
29202699	9	82	located	present	1391:1397	arg1	G27					1402:1404	G27	1402:1404	G27	1402:1404	On the other hand, high amount of Mannose was present in G27 in comparison to SS1.
29202699	9	82	located	present	1391:1397	arg2	amount					1369:1374	high amount	1364:1374	high amount of Mannose	1364:1385	On the other hand, high amount of Mannose was present in G27 in comparison to SS1.
29202699	5	83	with	strains	737:743	arg1	degree					770:775	varied degree	763:775	varied degree of virulence in human	763:797	Very little is known about the comparative LPS compositions of different strains of H. pylori with varied degree of virulence in human.
29202699	10	84	theme	differences	1505:1515	arg1	differences					1505:1515	differences	1505:1515	differences between the two strains, phenol layer of SS1	1505:1560	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	10	84	theme	differences	1505:1515	arg1	amount					1495:1500	considerable amount	1482:1500	considerable amount of differences between the two strains, phenol layer of SS1	1482:1560	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	7	85	theme	extraction	1000:1009	arg1	method					1011:1016	hot-phenol water extraction method	983:1016	hot-phenol water extraction method	983:1016	LPS were extracted from aqueous and phenol layer of hot-phenol water extraction method and subjected for composition analysis by gas chromatography - mass spectrometry (GC-MS) to sugar and fatty acid compositions.
29202699	9	86	theme	Mannose	1379:1385	arg1	Mannose					1379:1385	Mannose	1379:1385	Mannose	1379:1385	On the other hand, high amount of Mannose was present in G27 in comparison to SS1.
29202699	9	86	theme	Mannose	1379:1385	arg1	amount					1369:1374	high amount	1364:1374	high amount of Mannose	1364:1385	On the other hand, high amount of Mannose was present in G27 in comparison to SS1.
29202699	8	87	theme	Rhamnose	1230:1237	arg1	presence					1218:1225	the presence	1214:1225	the presence of Rhamnose, Fucose and GalNAc in the SS1 strain, which was either not found or with low abundance in the G27 strain	1214:1342	RESULTS The major difference between the two strains of H. pylori is the presence of Rhamnose, Fucose and GalNAc in the SS1 strain, which was either not found or with low abundance in the G27 strain.
29202699	8	87	theme	Rhamnose	1230:1237	arg1	difference					1163:1172	The major difference	1153:1172	The major difference between the two strains of H. pylori	1153:1209	RESULTS The major difference between the two strains of H. pylori is the presence of Rhamnose, Fucose and GalNAc in the SS1 strain, which was either not found or with low abundance in the G27 strain.
29202699	7	88	theme	hot-phenol	983:992	arg1	method					1011:1016	hot-phenol water extraction method	983:1016	hot-phenol water extraction method	983:1016	LPS were extracted from aqueous and phenol layer of hot-phenol water extraction method and subjected for composition analysis by gas chromatography - mass spectrometry (GC-MS) to sugar and fatty acid compositions.
29202699	7	89	theme	phenol	967:972	arg1	layer					974:978	aqueous and phenol layer	955:978	layer	974:978	LPS were extracted from aqueous and phenol layer of hot-phenol water extraction method and subjected for composition analysis by gas chromatography - mass spectrometry (GC-MS) to sugar and fatty acid compositions.
29202699	10	90	theme	acid	1647:1650	arg1	acid					1647:1650	3-hydroxy dodecanoic acid	1626:1650	3-hydroxy dodecanoic acid (3-OH-C12:0)	1626:1663	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	10	90	theme	acid	1647:1650	arg1	3-OH-C10:0					1610:1619	3-OH-C10:0	1610:1619	3-OH-C10:0	1610:1619	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	10	90	theme	acid	1647:1650	arg1	acid					1604:1607	3 hydroxy decanoic acid	1585:1607	3 hydroxy decanoic acid (3-OH-C10:0)	1585:1620	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	10	90	theme	acid	1647:1650	arg1	amount					1575:1580	enhanced amount	1566:1580	enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount	1566:1769	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	10	90	theme	acid	1647:1650	arg1	3-OH-C12:0					1653:1662	3-OH-C12:0	1653:1662	3-OH-C12:0	1653:1662	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	8	91	theme	Fucose	1240:1245	arg1	presence					1218:1225	the presence	1214:1225	the presence of Rhamnose, Fucose and GalNAc in the SS1 strain, which was either not found or with low abundance in the G27 strain	1214:1342	RESULTS The major difference between the two strains of H. pylori is the presence of Rhamnose, Fucose and GalNAc in the SS1 strain, which was either not found or with low abundance in the G27 strain.
29202699	8	91	theme	Fucose	1240:1245	arg1	difference					1163:1172	The major difference	1153:1172	The major difference between the two strains of H. pylori	1153:1209	RESULTS The major difference between the two strains of H. pylori is the presence of Rhamnose, Fucose and GalNAc in the SS1 strain, which was either not found or with low abundance in the G27 strain.
29202699	0	92	theme	Helicobacter	74:85	arg1	pylori					87:92	Helicobacter pylori	74:92	Helicobacter pylori	74:92	Comparison of lipopolysaccharides composition of two different strains of Helicobacter pylori.
29202699	11	93	theme	H.	1811:1812	arg1	analysis					1799:1806	The composition analysis	1783:1806	The composition analysis of H. pylori LPS,	1783:1824	CONCLUSION The composition analysis of H. pylori LPS, revealed differences in sugars and fatty acids composition between a mouse adapted strain SS1 and G27.
29202699	8	94	from	presence	1218:1225	arg1	strain					1269:1274	the SS1 strain	1261:1274	the SS1 strain	1261:1274	RESULTS The major difference between the two strains of H. pylori is the presence of Rhamnose, Fucose and GalNAc in the SS1 strain, which was either not found or with low abundance in the G27 strain.
29202699	8	94	from	presence	1218:1225	arg1	either					1287:1292	either	1287:1292	either	1287:1292	RESULTS The major difference between the two strains of H. pylori is the presence of Rhamnose, Fucose and GalNAc in the SS1 strain, which was either not found or with low abundance in the G27 strain.
29202699	8	95	theme	GalNAc	1251:1256	arg1	presence					1218:1225	the presence	1214:1225	the presence of Rhamnose, Fucose and GalNAc in the SS1 strain, which was either not found or with low abundance in the G27 strain	1214:1342	RESULTS The major difference between the two strains of H. pylori is the presence of Rhamnose, Fucose and GalNAc in the SS1 strain, which was either not found or with low abundance in the G27 strain.
29202699	8	95	theme	GalNAc	1251:1256	arg1	difference					1163:1172	The major difference	1153:1172	The major difference between the two strains of H. pylori	1153:1209	RESULTS The major difference between the two strains of H. pylori is the presence of Rhamnose, Fucose and GalNAc in the SS1 strain, which was either not found or with low abundance in the G27 strain.
29202699	5	96	from	degree	770:775	arg1	human					793:797	human	793:797	human	793:797	Very little is known about the comparative LPS compositions of different strains of H. pylori with varied degree of virulence in human.
29202699	11	97	theme	fatty	1861:1865	arg1	acids					1867:1871	fatty acids	1861:1871	fatty acids	1861:1871	CONCLUSION The composition analysis of H. pylori LPS, revealed differences in sugars and fatty acids composition between a mouse adapted strain SS1 and G27.
29202699	10	98	theme	enhanced	1566:1573	arg1	acid					1647:1650	3-hydroxy dodecanoic acid	1626:1650	3-hydroxy dodecanoic acid (3-OH-C12:0)	1626:1663	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	10	98	theme	enhanced	1566:1573	arg1	3-OH-C10:0					1610:1619	3-OH-C10:0	1610:1619	3-OH-C10:0	1610:1619	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	10	98	theme	enhanced	1566:1573	arg1	acid					1604:1607	3 hydroxy decanoic acid	1585:1607	3 hydroxy decanoic acid (3-OH-C10:0)	1585:1620	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	10	98	theme	enhanced	1566:1573	arg1	amount					1575:1580	enhanced amount	1566:1580	enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount	1566:1769	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	10	98	theme	enhanced	1566:1573	arg1	3-OH-C12:0					1653:1662	3-OH-C12:0	1653:1662	3-OH-C12:0	1653:1662	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	5	99	theme	different	727:735	arg1	strains					737:743	different strains	727:743	different strains of H. pylori with varied degree of virulence in human	727:797	Very little is known about the comparative LPS compositions of different strains of H. pylori with varied degree of virulence in human.
29202699	7	100	theme	aqueous	955:961	arg1	layer					974:978	aqueous and phenol layer	955:978	layer	974:978	LPS were extracted from aqueous and phenol layer of hot-phenol water extraction method and subjected for composition analysis by gas chromatography - mass spectrometry (GC-MS) to sugar and fatty acid compositions.
29202699	7	101	theme	acid	1126:1129	arg1	compositions					1131:1142	sugar and fatty acid compositions	1110:1142	compositions	1131:1142	LPS were extracted from aqueous and phenol layer of hot-phenol water extraction method and subjected for composition analysis by gas chromatography - mass spectrometry (GC-MS) to sugar and fatty acid compositions.
29202699	1	102	theme	BACKGROUND	95:104	arg1	H.					127:128	H.	127:128	H.	127:128	BACKGROUND Helicobacter pylori (H. pylori) is a Gram-negative, microaerophilic bacterium that is recognized as a major cause of chronic gastritis, peptic ulcers, and gastric cancer.
29202699	1	102	theme	BACKGROUND	95:104	arg1	pylori					119:124	BACKGROUND Helicobacter pylori	95:124	BACKGROUND Helicobacter pylori (H. pylori)	95:136	BACKGROUND Helicobacter pylori (H. pylori) is a Gram-negative, microaerophilic bacterium that is recognized as a major cause of chronic gastritis, peptic ulcers, and gastric cancer.
29202699	1	102	theme	BACKGROUND	95:104	arg1	bacterium					174:182	a Gram-negative, microaerophilic bacterium	141:182	bacterium	174:182	BACKGROUND Helicobacter pylori (H. pylori) is a Gram-negative, microaerophilic bacterium that is recognized as a major cause of chronic gastritis, peptic ulcers, and gastric cancer.
29202699	12	103	theme	further	2029:2035	arg1	studies					2037:2043	further studies	2029:2043	further studies	2029:2043	This knowledge provides a novel way to dissect out their importance in host-pathogen interaction in further studies.
29202699	2	104	theme	cellular	364:371	arg1	component					373:381	an important cellular component	351:381	an important cellular component of the outer membrane of H. pylori	351:416	Comparable to other Gram-negative bacteria, lipopolysaccharides (LPS) are an important cellular component of the outer membrane of H. pylori.
29202699	2	104	theme	cellular	364:371	arg1	lipopolysaccharides					321:339	lipopolysaccharides	321:339	lipopolysaccharides (LPS)	321:345	Comparable to other Gram-negative bacteria, lipopolysaccharides (LPS) are an important cellular component of the outer membrane of H. pylori.
29202699	10	105	located	present	1680:1686	arg2	3-OH-C10:0					1610:1619	3-OH-C10:0	1610:1619	3-OH-C10:0	1610:1619	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	10	105	located	present	1680:1686	arg2	acid					1647:1650	3-hydroxy dodecanoic acid	1626:1650	3-hydroxy dodecanoic acid (3-OH-C12:0)	1626:1663	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	10	105	located	present	1680:1686	arg2	acid					1604:1607	3 hydroxy decanoic acid	1585:1607	3 hydroxy decanoic acid (3-OH-C10:0)	1585:1620	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	10	105	located	present	1680:1686	arg1	G27					1691:1693	G27	1691:1693	G27	1691:1693	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	10	105	located	present	1680:1686	arg2	3-OH-C12:0					1653:1662	3-OH-C12:0	1653:1662	3-OH-C12:0	1653:1662	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	10	105	located	present	1680:1686	arg2	amount					1575:1580	enhanced amount	1566:1580	enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount	1566:1769	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	1	106	theme	cancer	269:274	arg1	bacterium					174:182	a Gram-negative, microaerophilic bacterium	141:182	bacterium	174:182	BACKGROUND Helicobacter pylori (H. pylori) is a Gram-negative, microaerophilic bacterium that is recognized as a major cause of chronic gastritis, peptic ulcers, and gastric cancer.
29202699	1	106	theme	cancer	269:274	arg1	cause					214:218	a major cause	206:218	a major cause of chronic gastritis, peptic ulcers, and gastric cancer	206:274	BACKGROUND Helicobacter pylori (H. pylori) is a Gram-negative, microaerophilic bacterium that is recognized as a major cause of chronic gastritis, peptic ulcers, and gastric cancer.
29202699	10	107	from	present	1680:1686	arg1	G27					1691:1693	G27	1691:1693	G27	1691:1693	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	2	108	theme	pylori	411:416	arg1	membrane					396:403	the outer membrane	386:403	the outer membrane of H. pylori	386:416	Comparable to other Gram-negative bacteria, lipopolysaccharides (LPS) are an important cellular component of the outer membrane of H. pylori.
29202699	10	109	theme	SS1	1558:1560	arg1	strains					1533:1539	the two strains	1525:1539	the two strains	1525:1539	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	10	109	theme	SS1	1558:1560	arg1	layer					1549:1553	phenol layer	1542:1553	phenol layer of SS1	1542:1560	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	5	110	theme	varied	763:768	arg1	degree					770:775	varied degree	763:775	varied degree of virulence in human	763:797	Very little is known about the comparative LPS compositions of different strains of H. pylori with varied degree of virulence in human.
29202699	10	111	from	G27	1691:1693	arg1	present					1680:1686	present	1680:1686	present	1680:1686	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	9	112	attach	present	1391:1397	arg1	hand					1358:1361	the other hand	1348:1361	the other hand	1348:1361	On the other hand, high amount of Mannose was present in G27 in comparison to SS1.
29202699	9	112	attach	present	1391:1397	arg2	amount					1369:1374	high amount	1364:1374	high amount of Mannose	1364:1385	On the other hand, high amount of Mannose was present in G27 in comparison to SS1.
29202699	9	112	attach	present	1391:1397	arg1	comparison					1409:1418	comparison	1409:1418	comparison to SS1	1409:1425	On the other hand, high amount of Mannose was present in G27 in comparison to SS1.
29202699	9	112	attach	present	1391:1397	arg1	G27					1402:1404	G27	1402:1404	G27	1402:1404	On the other hand, high amount of Mannose was present in G27 in comparison to SS1.
29202699	9	112	attach	present	1391:1397	arg2	Mannose					1379:1385	Mannose	1379:1385	Mannose	1379:1385	On the other hand, high amount of Mannose was present in G27 in comparison to SS1.
29202699	10	113	theme	myristic	1704:1711	arg1	acid					1713:1716	myristic acid	1704:1716	myristic acid (C14:0)	1704:1724	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	10	113	theme	myristic	1704:1711	arg1	C14:0					1719:1723	C14:0	1719:1723	C14:0	1719:1723	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	2	114	theme	outer	390:394	arg1	membrane					396:403	the outer membrane	386:403	the outer membrane of H. pylori	386:416	Comparable to other Gram-negative bacteria, lipopolysaccharides (LPS) are an important cellular component of the outer membrane of H. pylori.
29202699	0	115	theme	strains	63:69	arg1	composition					34:44	composition	34:44	composition of two different strains of Helicobacter pylori	34:92	Comparison of lipopolysaccharides composition of two different strains of Helicobacter pylori.
29202699	5	116	dep	H.	748:749	arg1	pylori					751:756	H. pylori	748:756	H. pylori	748:756	Very little is known about the comparative LPS compositions of different strains of H. pylori with varied degree of virulence in human.
29202699	0	117	dep	composition	34:44	arg1	Comparison					0:9	Comparison	0:9	Comparison of lipopolysaccharides	0:32	Comparison of lipopolysaccharides composition of two different strains of Helicobacter pylori.
29202699	4	118	theme	inflammatory	572:583	arg1	responses					585:593	pathogen-induced host inflammatory responses	550:593	pathogen-induced host inflammatory responses resulting in chronic inflammation within the gastrointestinal tract	550:661	In addition, H. pylori LPS modulates pathogen-induced host inflammatory responses resulting in chronic inflammation within the gastrointestinal tract.
29202699	3	119	theme	key	452:454	arg1	role					456:459	a key role	450:459	a key role	450:459	The LPS of this organism plays a key role in its colonization and persistence in the stomach.
29202699	1	120	theme	chronic	223:229	arg1	gastritis					231:239	chronic gastritis	223:239	chronic gastritis	223:239	BACKGROUND Helicobacter pylori (H. pylori) is a Gram-negative, microaerophilic bacterium that is recognized as a major cause of chronic gastritis, peptic ulcers, and gastric cancer.
29202699	12	121	theme	novel	1955:1959	arg1	way					1961:1963	a novel way	1953:1963	a novel way to dissect out their importance in host-pathogen interaction in further studies	1953:2043	This knowledge provides a novel way to dissect out their importance in host-pathogen interaction in further studies.
29202699	10	122	theme	Fatty	1428:1432	arg1	composition					1439:1449	Fatty acid composition	1428:1449	Fatty acid composition of lipid-A portion	1428:1468	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	10	123	theme	acid	1604:1607	arg1	acid					1647:1650	3-hydroxy dodecanoic acid	1626:1650	3-hydroxy dodecanoic acid (3-OH-C12:0)	1626:1663	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	10	123	theme	acid	1604:1607	arg1	3-OH-C10:0					1610:1619	3-OH-C10:0	1610:1619	3-OH-C10:0	1610:1619	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	10	123	theme	acid	1604:1607	arg1	acid					1604:1607	3 hydroxy decanoic acid	1585:1607	3 hydroxy decanoic acid (3-OH-C10:0)	1585:1620	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	10	123	theme	acid	1604:1607	arg1	amount					1575:1580	enhanced amount	1566:1580	enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount	1566:1769	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	10	123	theme	acid	1604:1607	arg1	3-OH-C12:0					1653:1662	3-OH-C12:0	1653:1662	3-OH-C12:0	1653:1662	Fatty acid composition of lipid-A portion also showed considerable amount of differences between the two strains, phenol layer of SS1 had enhanced amount of 3 hydroxy decanoic acid (3-OH-C10:0) and 3-hydroxy dodecanoic acid (3-OH-C12:0) which were not present in G27, whereas myristic acid (C14:0) was present in G27 in relatively high amount.
29202699	12	124	from	importance	1986:1995	arg1	interaction					2014:2024	host-pathogen interaction	2000:2024	host-pathogen interaction	2000:2024	This knowledge provides a novel way to dissect out their importance in host-pathogen interaction in further studies.
29202699	12	124	from	importance	1986:1995	arg1	studies					2037:2043	further studies	2029:2043	further studies	2029:2043	This knowledge provides a novel way to dissect out their importance in host-pathogen interaction in further studies.
26041171	1	0	theme	bioprocesses	283:294	arg1	development					268:278	the development	264:278	the development of bioprocesses related to enzyme applications	264:325	The objective of this study was to produce a composite biocatalyst, based on porous cellulosic material, produced after wood sawdust delignification (tubular cellulose; TC) and starch gel (SG), for the development of bioprocesses related to enzyme applications.
26041171	5	1	theme	several	995:1001	arg1	applications					1003:1014	several applications	995:1014	several applications in food enzyme research and technology	995:1053	The composite was used as an insoluble carrier for entrapment of the dairy enzyme rennin, leading to the production of an active biocatalyst for milk coagulation (initiation of milk clotting at about 20 min and full coagulation at about 200 min), creating perspectives for several applications in food enzyme research and technology.
26041171	1	2	theme	related	296:302	arg1	bioprocesses					283:294	bioprocesses	283:294	bioprocesses related to enzyme applications	283:325	The objective of this study was to produce a composite biocatalyst, based on porous cellulosic material, produced after wood sawdust delignification (tubular cellulose; TC) and starch gel (SG), for the development of bioprocesses related to enzyme applications.
26041171	1	3	theme	porous	143:148	arg1	material					161:168	porous cellulosic material	143:168	porous cellulosic material	143:168	The objective of this study was to produce a composite biocatalyst, based on porous cellulosic material, produced after wood sawdust delignification (tubular cellulose; TC) and starch gel (SG), for the development of bioprocesses related to enzyme applications.
26041171	5	4	from	applications	1003:1014	arg1	research					1031:1038	food enzyme research	1019:1038	food enzyme research	1019:1038	The composite was used as an insoluble carrier for entrapment of the dairy enzyme rennin, leading to the production of an active biocatalyst for milk coagulation (initiation of milk clotting at about 20 min and full coagulation at about 200 min), creating perspectives for several applications in food enzyme research and technology.
26041171	5	4	from	applications	1003:1014	arg1	technology					1044:1053	technology	1044:1053	technology	1044:1053	The composite was used as an insoluble carrier for entrapment of the dairy enzyme rennin, leading to the production of an active biocatalyst for milk coagulation (initiation of milk clotting at about 20 min and full coagulation at about 200 min), creating perspectives for several applications in food enzyme research and technology.
26041171	1	5	theme	enzyme	307:312	arg1	applications					314:325	enzyme applications	307:325	enzyme applications	307:325	The objective of this study was to produce a composite biocatalyst, based on porous cellulosic material, produced after wood sawdust delignification (tubular cellulose; TC) and starch gel (SG), for the development of bioprocesses related to enzyme applications.
26041171	4	6	theme	crystallinity	666:678	arg1	decrease					654:661	a decrease	652:661	a decrease of crystallinity	652:678	X-ray powder diffractometry showed a decrease of crystallinity with increased SG ratio in the composite.
26041171	1	7	theme	starch	243:248	arg1	SG					255:256	SG	255:256	SG	255:256	The objective of this study was to produce a composite biocatalyst, based on porous cellulosic material, produced after wood sawdust delignification (tubular cellulose; TC) and starch gel (SG), for the development of bioprocesses related to enzyme applications.
26041171	1	7	theme	starch	243:248	arg1	gel					250:252	starch gel	243:252	starch gel (SG)	243:257	The objective of this study was to produce a composite biocatalyst, based on porous cellulosic material, produced after wood sawdust delignification (tubular cellulose; TC) and starch gel (SG), for the development of bioprocesses related to enzyme applications.
26041171	2	8	from	deposition	416:425	arg1	pores					437:441	the TC pores	430:441	the TC pores	430:441	The composite biocatalyst was studied by Scanning Electron Microscopy to observe the SG deposition in the TC pores, and porosimetry analysis to determine the average pore diameter and surface area.
26041171	2	9	theme	Electron	378:385	arg1	Microscopy					387:396	Scanning Electron Microscopy to observe the SG deposition in the TC pores, and porosimetry analysis to determine the average pore diameter and surface area	369:523	Scanning Electron Microscopy to observe the SG deposition in the TC pores, and porosimetry analysis to determine the average pore diameter and surface area	369:523	The composite biocatalyst was studied by Scanning Electron Microscopy to observe the SG deposition in the TC pores, and porosimetry analysis to determine the average pore diameter and surface area.
26041171	5	10	theme	milk	899:902	arg1	coagulation					938:948	full coagulation	933:948	full coagulation	933:948	The composite was used as an insoluble carrier for entrapment of the dairy enzyme rennin, leading to the production of an active biocatalyst for milk coagulation (initiation of milk clotting at about 20 min and full coagulation at about 200 min), creating perspectives for several applications in food enzyme research and technology.
26041171	5	10	theme	milk	899:902	arg1	initiation					885:894	initiation	885:894	initiation of milk clotting at about 20 min	885:927	The composite was used as an insoluble carrier for entrapment of the dairy enzyme rennin, leading to the production of an active biocatalyst for milk coagulation (initiation of milk clotting at about 20 min and full coagulation at about 200 min), creating perspectives for several applications in food enzyme research and technology.
26041171	5	11	theme	active	844:849	arg1	biocatalyst					851:861	an active biocatalyst	841:861	an active biocatalyst for milk coagulation (initiation of milk clotting at about 20 min and full coagulation at about 200 min)	841:966	The composite was used as an insoluble carrier for entrapment of the dairy enzyme rennin, leading to the production of an active biocatalyst for milk coagulation (initiation of milk clotting at about 20 min and full coagulation at about 200 min), creating perspectives for several applications in food enzyme research and technology.
26041171	1	12	theme	cellulosic	150:159	arg1	material					161:168	porous cellulosic material	143:168	porous cellulosic material	143:168	The objective of this study was to produce a composite biocatalyst, based on porous cellulosic material, produced after wood sawdust delignification (tubular cellulose; TC) and starch gel (SG), for the development of bioprocesses related to enzyme applications.
26041171	2	13	theme	TC	434:435	arg1	pores					437:441	the TC pores	430:441	the TC pores	430:441	The composite biocatalyst was studied by Scanning Electron Microscopy to observe the SG deposition in the TC pores, and porosimetry analysis to determine the average pore diameter and surface area.
26041171	4	14	from	ratio	698:702	arg1	composite					711:719	the composite	707:719	the composite	707:719	X-ray powder diffractometry showed a decrease of crystallinity with increased SG ratio in the composite.
26041171	5	15	theme	enzyme	797:802	arg1	rennin					804:809	the dairy enzyme rennin	787:809	the dairy enzyme rennin	787:809	The composite was used as an insoluble carrier for entrapment of the dairy enzyme rennin, leading to the production of an active biocatalyst for milk coagulation (initiation of milk clotting at about 20 min and full coagulation at about 200 min), creating perspectives for several applications in food enzyme research and technology.
26041171	2	16	theme	SG	413:414	arg1	deposition					416:425	the SG deposition	409:425	the SG deposition in the TC pores	409:441	The composite biocatalyst was studied by Scanning Electron Microscopy to observe the SG deposition in the TC pores, and porosimetry analysis to determine the average pore diameter and surface area.
26041171	5	17	theme	food	1019:1022	arg1	research					1031:1038	food enzyme research	1019:1038	food enzyme research	1019:1038	The composite was used as an insoluble carrier for entrapment of the dairy enzyme rennin, leading to the production of an active biocatalyst for milk coagulation (initiation of milk clotting at about 20 min and full coagulation at about 200 min), creating perspectives for several applications in food enzyme research and technology.
26041171	5	18	from	min	963:965	arg1	coagulation					938:948	full coagulation	933:948	full coagulation	933:948	The composite was used as an insoluble carrier for entrapment of the dairy enzyme rennin, leading to the production of an active biocatalyst for milk coagulation (initiation of milk clotting at about 20 min and full coagulation at about 200 min), creating perspectives for several applications in food enzyme research and technology.
26041171	5	18	from	min	963:965	arg1	initiation					885:894	initiation	885:894	initiation of milk clotting at about 20 min	885:927	The composite was used as an insoluble carrier for entrapment of the dairy enzyme rennin, leading to the production of an active biocatalyst for milk coagulation (initiation of milk clotting at about 20 min and full coagulation at about 200 min), creating perspectives for several applications in food enzyme research and technology.
26041171	4	19	theme	increased	685:693	arg1	ratio					698:702	increased SG ratio	685:702	increased SG ratio in the composite	685:719	X-ray powder diffractometry showed a decrease of crystallinity with increased SG ratio in the composite.
26041171	5	20	theme	rennin	804:809	arg1	entrapment					773:782	entrapment	773:782	entrapment	773:782	The composite was used as an insoluble carrier for entrapment of the dairy enzyme rennin, leading to the production of an active biocatalyst for milk coagulation (initiation of milk clotting at about 20 min and full coagulation at about 200 min), creating perspectives for several applications in food enzyme research and technology.
26041171	1	21	theme	study	88:92	arg1	objective					70:78	The objective	66:78	The objective of this study	66:92	The objective of this study was to produce a composite biocatalyst, based on porous cellulosic material, produced after wood sawdust delignification (tubular cellulose; TC) and starch gel (SG), for the development of bioprocesses related to enzyme applications.
26041171	2	22	theme	composite	332:340	arg1	biocatalyst					342:352	The composite biocatalyst	328:352	The composite biocatalyst	328:352	The composite biocatalyst was studied by Scanning Electron Microscopy to observe the SG deposition in the TC pores, and porosimetry analysis to determine the average pore diameter and surface area.
26041171	5	23	theme	insoluble	751:759	arg1	composite					726:734	The composite	722:734	The composite	722:734	The composite was used as an insoluble carrier for entrapment of the dairy enzyme rennin, leading to the production of an active biocatalyst for milk coagulation (initiation of milk clotting at about 20 min and full coagulation at about 200 min), creating perspectives for several applications in food enzyme research and technology.
26041171	5	23	theme	insoluble	751:759	arg1	carrier					761:767	an insoluble carrier	748:767	an insoluble carrier for entrapment of the dairy enzyme rennin, leading to the production of an active biocatalyst for milk coagulation (initiation of milk clotting at about 20 min and full coagulation at about 200 min), creating perspectives for several applications in food enzyme research and technology	748:1053	The composite was used as an insoluble carrier for entrapment of the dairy enzyme rennin, leading to the production of an active biocatalyst for milk coagulation (initiation of milk clotting at about 20 min and full coagulation at about 200 min), creating perspectives for several applications in food enzyme research and technology.
26041171	5	24	used	used	740:743	arg2	carrier					761:767	an insoluble carrier	748:767	an insoluble carrier for entrapment of the dairy enzyme rennin, leading to the production of an active biocatalyst for milk coagulation (initiation of milk clotting at about 20 min and full coagulation at about 200 min), creating perspectives for several applications in food enzyme research and technology	748:1053	The composite was used as an insoluble carrier for entrapment of the dairy enzyme rennin, leading to the production of an active biocatalyst for milk coagulation (initiation of milk clotting at about 20 min and full coagulation at about 200 min), creating perspectives for several applications in food enzyme research and technology.
26041171	5	24	used	used	740:743	arg2	composite					726:734	The composite	722:734	The composite	722:734	The composite was used as an insoluble carrier for entrapment of the dairy enzyme rennin, leading to the production of an active biocatalyst for milk coagulation (initiation of milk clotting at about 20 min and full coagulation at about 200 min), creating perspectives for several applications in food enzyme research and technology.
26041171	0	25	theme	gel	25:27	arg1	composite					29:37	gel composite	25:37	gel composite	25:37	Tubular cellulose/starch gel composite as food enzyme storehouse.
26041171	5	26	theme	milk	867:870	arg1	coagulation					872:882	milk coagulation	867:882	milk coagulation (initiation of milk clotting at about 20 min and full coagulation at about 200 min)	867:966	The composite was used as an insoluble carrier for entrapment of the dairy enzyme rennin, leading to the production of an active biocatalyst for milk coagulation (initiation of milk clotting at about 20 min and full coagulation at about 200 min), creating perspectives for several applications in food enzyme research and technology.
26041171	4	27	theme	SG	695:696	arg1	ratio					698:702	increased SG ratio	685:702	increased SG ratio in the composite	685:719	X-ray powder diffractometry showed a decrease of crystallinity with increased SG ratio in the composite.
26041171	3	28	with	composite	582:590	arg1	sizes					610:614	reduced pore sizes	597:614	reduced pore sizes	597:614	The deposition of SG into the TC tubes provided a TC/SG composite with reduced pore sizes.
26041171	1	29	theme	wood	186:189	arg1	TC					235:236	tubular cellulose; TC	216:236	tubular cellulose; TC	216:236	The objective of this study was to produce a composite biocatalyst, based on porous cellulosic material, produced after wood sawdust delignification (tubular cellulose; TC) and starch gel (SG), for the development of bioprocesses related to enzyme applications.
26041171	1	29	theme	wood	186:189	arg1	delignification					199:213	wood sawdust delignification	186:213	wood sawdust delignification (tubular cellulose; TC)	186:237	The objective of this study was to produce a composite biocatalyst, based on porous cellulosic material, produced after wood sawdust delignification (tubular cellulose; TC) and starch gel (SG), for the development of bioprocesses related to enzyme applications.
26041171	0	30	theme	food	42:45	arg1	storehouse					54:63	food enzyme storehouse	42:63	food enzyme storehouse	42:63	Tubular cellulose/starch gel composite as food enzyme storehouse.
26041171	2	31	theme	pore	494:497	arg1	diameter					499:506	the average pore diameter	482:506	the average pore diameter	482:506	The composite biocatalyst was studied by Scanning Electron Microscopy to observe the SG deposition in the TC pores, and porosimetry analysis to determine the average pore diameter and surface area.
26041171	1	32	theme	sawdust	191:197	arg1	TC					235:236	tubular cellulose; TC	216:236	tubular cellulose; TC	216:236	The objective of this study was to produce a composite biocatalyst, based on porous cellulosic material, produced after wood sawdust delignification (tubular cellulose; TC) and starch gel (SG), for the development of bioprocesses related to enzyme applications.
26041171	1	32	theme	sawdust	191:197	arg1	delignification					199:213	wood sawdust delignification	186:213	wood sawdust delignification (tubular cellulose; TC)	186:237	The objective of this study was to produce a composite biocatalyst, based on porous cellulosic material, produced after wood sawdust delignification (tubular cellulose; TC) and starch gel (SG), for the development of bioprocesses related to enzyme applications.
26041171	3	33	theme	SG	544:545	arg1	deposition					530:539	The deposition	526:539	The deposition of SG into the TC tubes	526:563	The deposition of SG into the TC tubes provided a TC/SG composite with reduced pore sizes.
26041171	2	34	theme	Scanning	369:376	arg1	Microscopy					387:396	Scanning Electron Microscopy to observe the SG deposition in the TC pores, and porosimetry analysis to determine the average pore diameter and surface area	369:523	Scanning Electron Microscopy to observe the SG deposition in the TC pores, and porosimetry analysis to determine the average pore diameter and surface area	369:523	The composite biocatalyst was studied by Scanning Electron Microscopy to observe the SG deposition in the TC pores, and porosimetry analysis to determine the average pore diameter and surface area.
26041171	5	35	theme	dairy	791:795	arg1	rennin					804:809	the dairy enzyme rennin	787:809	the dairy enzyme rennin	787:809	The composite was used as an insoluble carrier for entrapment of the dairy enzyme rennin, leading to the production of an active biocatalyst for milk coagulation (initiation of milk clotting at about 20 min and full coagulation at about 200 min), creating perspectives for several applications in food enzyme research and technology.
26041171	2	36	theme	average	486:492	arg1	diameter					499:506	the average pore diameter	482:506	the average pore diameter	482:506	The composite biocatalyst was studied by Scanning Electron Microscopy to observe the SG deposition in the TC pores, and porosimetry analysis to determine the average pore diameter and surface area.
26041171	5	37	theme	full	933:936	arg1	coagulation					938:948	full coagulation	933:948	full coagulation	933:948	The composite was used as an insoluble carrier for entrapment of the dairy enzyme rennin, leading to the production of an active biocatalyst for milk coagulation (initiation of milk clotting at about 20 min and full coagulation at about 200 min), creating perspectives for several applications in food enzyme research and technology.
26041171	3	38	theme	TC/SG	576:580	arg1	composite					582:590	a TC/SG composite	574:590	a TC/SG composite with reduced pore sizes	574:614	The deposition of SG into the TC tubes provided a TC/SG composite with reduced pore sizes.
26041171	2	39	dep	Microscopy	387:396	arg1	determine					472:480	determine	472:480	to determine the average pore diameter and surface area	469:523	The composite biocatalyst was studied by Scanning Electron Microscopy to observe the SG deposition in the TC pores, and porosimetry analysis to determine the average pore diameter and surface area.
26041171	2	39	dep	Microscopy	387:396	arg1	observe					401:407	observe	401:407	to observe the SG deposition in the TC pores	398:441	The composite biocatalyst was studied by Scanning Electron Microscopy to observe the SG deposition in the TC pores, and porosimetry analysis to determine the average pore diameter and surface area.
26041171	0	40	theme	enzyme	47:52	arg1	storehouse					54:63	food enzyme storehouse	42:63	food enzyme storehouse	42:63	Tubular cellulose/starch gel composite as food enzyme storehouse.
26041171	1	41	theme	composite	111:119	arg1	biocatalyst					121:131	a composite biocatalyst	109:131	a composite biocatalyst	109:131	The objective of this study was to produce a composite biocatalyst, based on porous cellulosic material, produced after wood sawdust delignification (tubular cellulose; TC) and starch gel (SG), for the development of bioprocesses related to enzyme applications.
26041171	2	42	theme	surface	512:518	arg1	area					520:523	surface area	512:523	surface area	512:523	The composite biocatalyst was studied by Scanning Electron Microscopy to observe the SG deposition in the TC pores, and porosimetry analysis to determine the average pore diameter and surface area.
26041171	3	43	theme	reduced	597:603	arg1	sizes					610:614	reduced pore sizes	597:614	reduced pore sizes	597:614	The deposition of SG into the TC tubes provided a TC/SG composite with reduced pore sizes.
26041171	5	44	dep	coagulation	872:882	arg1	coagulation					938:948	full coagulation	933:948	full coagulation	933:948	The composite was used as an insoluble carrier for entrapment of the dairy enzyme rennin, leading to the production of an active biocatalyst for milk coagulation (initiation of milk clotting at about 20 min and full coagulation at about 200 min), creating perspectives for several applications in food enzyme research and technology.
26041171	5	44	dep	coagulation	872:882	arg1	initiation					885:894	initiation	885:894	initiation of milk clotting at about 20 min	885:927	The composite was used as an insoluble carrier for entrapment of the dairy enzyme rennin, leading to the production of an active biocatalyst for milk coagulation (initiation of milk clotting at about 20 min and full coagulation at about 200 min), creating perspectives for several applications in food enzyme research and technology.
26041171	5	45	theme	biocatalyst	851:861	arg1	production					827:836	the production	823:836	the production of an active biocatalyst for milk coagulation (initiation of milk clotting at about 20 min and full coagulation at about 200 min)	823:966	The composite was used as an insoluble carrier for entrapment of the dairy enzyme rennin, leading to the production of an active biocatalyst for milk coagulation (initiation of milk clotting at about 20 min and full coagulation at about 200 min), creating perspectives for several applications in food enzyme research and technology.
26041171	1	46	theme	tubular	216:222	arg1	TC					235:236	tubular cellulose; TC	216:236	tubular cellulose; TC	216:236	The objective of this study was to produce a composite biocatalyst, based on porous cellulosic material, produced after wood sawdust delignification (tubular cellulose; TC) and starch gel (SG), for the development of bioprocesses related to enzyme applications.
26041171	1	46	theme	tubular	216:222	arg1	delignification					199:213	wood sawdust delignification	186:213	wood sawdust delignification (tubular cellulose; TC)	186:237	The objective of this study was to produce a composite biocatalyst, based on porous cellulosic material, produced after wood sawdust delignification (tubular cellulose; TC) and starch gel (SG), for the development of bioprocesses related to enzyme applications.
26041171	2	47	theme	porosimetry	448:458	arg1	analysis					460:467	porosimetry analysis	448:467	porosimetry analysis	448:467	The composite biocatalyst was studied by Scanning Electron Microscopy to observe the SG deposition in the TC pores, and porosimetry analysis to determine the average pore diameter and surface area.
26041171	3	48	theme	pore	605:608	arg1	sizes					610:614	reduced pore sizes	597:614	reduced pore sizes	597:614	The deposition of SG into the TC tubes provided a TC/SG composite with reduced pore sizes.
26041171	4	49	theme	powder	623:628	arg1	diffractometry					630:643	X-ray powder diffractometry	617:643	X-ray powder diffractometry	617:643	X-ray powder diffractometry showed a decrease of crystallinity with increased SG ratio in the composite.
26041171	1	50	theme	cellulose	224:232	arg1	TC					235:236	tubular cellulose; TC	216:236	tubular cellulose; TC	216:236	The objective of this study was to produce a composite biocatalyst, based on porous cellulosic material, produced after wood sawdust delignification (tubular cellulose; TC) and starch gel (SG), for the development of bioprocesses related to enzyme applications.
26041171	1	50	theme	cellulose	224:232	arg1	delignification					199:213	wood sawdust delignification	186:213	wood sawdust delignification (tubular cellulose; TC)	186:237	The objective of this study was to produce a composite biocatalyst, based on porous cellulosic material, produced after wood sawdust delignification (tubular cellulose; TC) and starch gel (SG), for the development of bioprocesses related to enzyme applications.
26041171	5	51	theme	enzyme	1024:1029	arg1	research					1031:1038	food enzyme research	1019:1038	food enzyme research	1019:1038	The composite was used as an insoluble carrier for entrapment of the dairy enzyme rennin, leading to the production of an active biocatalyst for milk coagulation (initiation of milk clotting at about 20 min and full coagulation at about 200 min), creating perspectives for several applications in food enzyme research and technology.
26041171	3	52	theme	TC	556:557	arg1	tubes					559:563	the TC tubes	552:563	the TC tubes	552:563	The deposition of SG into the TC tubes provided a TC/SG composite with reduced pore sizes.
26041171	4	53	theme	X-ray	617:621	arg1	diffractometry					630:643	X-ray powder diffractometry	617:643	X-ray powder diffractometry	617:643	X-ray powder diffractometry showed a decrease of crystallinity with increased SG ratio in the composite.
27922663	2	0	from	essential	199:207	arg1	formation					220:228	biofilm formation	212:228	biofilm formation of certain bacteria	212:248	Polyamines are essential in biofilm formation of certain bacteria.
27922663	6	1	theme	assembly	733:740	arg1	pattern					742:748	bacterial assembly pattern	723:748	bacterial assembly pattern in S. mutans biofilms	723:770	The different architectures of the biofilms in norspermidine and control groups indicated that the basic units, bacteria‑exopolysaccharide units (BEUs), represent the exopolysaccharide (EPS) and bacterial assembly pattern in S. mutans biofilms.
27922663	6	2	theme	bacteria‑exopolysaccharide	640:665	arg1	BEUs					674:677	BEUs	674:677	BEUs	674:677	The different architectures of the biofilms in norspermidine and control groups indicated that the basic units, bacteria‑exopolysaccharide units (BEUs), represent the exopolysaccharide (EPS) and bacterial assembly pattern in S. mutans biofilms.
27922663	6	2	theme	bacteria‑exopolysaccharide	640:665	arg1	units					633:637	the basic units	623:637	the basic units	623:637	The different architectures of the biofilms in norspermidine and control groups indicated that the basic units, bacteria‑exopolysaccharide units (BEUs), represent the exopolysaccharide (EPS) and bacterial assembly pattern in S. mutans biofilms.
27922663	6	2	theme	bacteria‑exopolysaccharide	640:665	arg1	units					667:671	bacteria‑exopolysaccharide units	640:671	bacteria‑exopolysaccharide units (BEUs)	640:678	The different architectures of the biofilms in norspermidine and control groups indicated that the basic units, bacteria‑exopolysaccharide units (BEUs), represent the exopolysaccharide (EPS) and bacterial assembly pattern in S. mutans biofilms.
27922663	9	3	theme	irregular	1141:1149	arg1	architecture					1151:1162	an irregular architecture	1138:1162	an irregular architecture in the norspermidine group	1138:1189	Gene‑expression microarray analysis indicated that the formation of an irregular architecture in the norspermidine group was potentially attributable to the downregulation of elements of the quorum‑sensing system (by 2.7‑15‑fold).
27922663	10	4	theme	basic	1349:1353	arg1	structure					1355:1363	a basic structure	1347:1363	a basic structure of S. mutans biofilm	1347:1384	The present study suggested that the BEUs are a basic structure of S. mutans biofilm and its assembly is regulated majorly by the quorum‑sensing system.
27922663	10	4	theme	basic	1349:1353	arg1	BEUs					1338:1341	the BEUs	1334:1341	the BEUs	1334:1341	The present study suggested that the BEUs are a basic structure of S. mutans biofilm and its assembly is regulated majorly by the quorum‑sensing system.
27922663	6	5	from	groups	601:606	arg1	architectures					542:554	The different architectures	528:554	The different architectures of the biofilms in norspermidine and control groups	528:606	The different architectures of the biofilms in norspermidine and control groups indicated that the basic units, bacteria‑exopolysaccharide units (BEUs), represent the exopolysaccharide (EPS) and bacterial assembly pattern in S. mutans biofilms.
27922663	4	6	theme	mutans	402:407	arg1	biofilms					409:416	S. mutans biofilms	399:416	S. mutans biofilms	399:416	However, the role of norspermidine in S. mutans biofilms remains unknown.
27922663	9	7	theme	Gene‑expression	1070:1084	arg1	analysis					1097:1104	Gene‑expression microarray analysis	1070:1104	Gene‑expression microarray analysis	1070:1104	Gene‑expression microarray analysis indicated that the formation of an irregular architecture in the norspermidine group was potentially attributable to the downregulation of elements of the quorum‑sensing system (by 2.7‑15‑fold).
27922663	6	8	from	biofilms	563:570	arg1	groups					601:606	norspermidine and control groups	575:606	groups	601:606	The different architectures of the biofilms in norspermidine and control groups indicated that the basic units, bacteria‑exopolysaccharide units (BEUs), represent the exopolysaccharide (EPS) and bacterial assembly pattern in S. mutans biofilms.
27922663	8	9	theme	biofilm	1051:1057	arg1	structure					1059:1067	the biofilm structure	1047:1067	the biofilm structure	1047:1067	Therefore, 5 mM norspermidine inhibited biofilm formation both by decreasing the rate of cell viability and changing the biofilm structure.
27922663	4	10	theme	S.	399:400	arg1	biofilms					409:416	S. mutans biofilms	399:416	S. mutans biofilms	399:416	However, the role of norspermidine in S. mutans biofilms remains unknown.
27922663	6	11	theme	norspermidine	575:587	arg1	groups					601:606	norspermidine and control groups	575:606	groups	601:606	The different architectures of the biofilms in norspermidine and control groups indicated that the basic units, bacteria‑exopolysaccharide units (BEUs), represent the exopolysaccharide (EPS) and bacterial assembly pattern in S. mutans biofilms.
27922663	6	12	from	exopolysaccharide	695:711	arg1	biofilms					763:770	S. mutans biofilms	753:770	S. mutans biofilms	753:770	The different architectures of the biofilms in norspermidine and control groups indicated that the basic units, bacteria‑exopolysaccharide units (BEUs), represent the exopolysaccharide (EPS) and bacterial assembly pattern in S. mutans biofilms.
27922663	6	13	theme	different	532:540	arg1	architectures					542:554	The different architectures	528:554	The different architectures of the biofilms in norspermidine and control groups	528:606	The different architectures of the biofilms in norspermidine and control groups indicated that the basic units, bacteria‑exopolysaccharide units (BEUs), represent the exopolysaccharide (EPS) and bacterial assembly pattern in S. mutans biofilms.
27922663	10	14	theme	S.	1368:1369	arg1	biofilm					1378:1384	S. mutans biofilm	1368:1384	S. mutans biofilm	1368:1384	The present study suggested that the BEUs are a basic structure of S. mutans biofilm and its assembly is regulated majorly by the quorum‑sensing system.
27922663	6	15	theme	bacterial	723:731	arg1	pattern					742:748	bacterial assembly pattern	723:748	bacterial assembly pattern in S. mutans biofilms	723:770	The different architectures of the biofilms in norspermidine and control groups indicated that the basic units, bacteria‑exopolysaccharide units (BEUs), represent the exopolysaccharide (EPS) and bacterial assembly pattern in S. mutans biofilms.
27922663	3	16	theme	unusual	269:275	arg1	Norspermidine					251:263	Norspermidine	251:263	Norspermidine	251:263	Norspermidine, an unusual polyamine, has been a controversial polyamine that can lead to biofilm disassembly.
27922663	3	16	theme	unusual	269:275	arg1	polyamine					277:285	an unusual polyamine	266:285	an unusual polyamine	266:285	Norspermidine, an unusual polyamine, has been a controversial polyamine that can lead to biofilm disassembly.
27922663	5	17	theme	S.	508:509	arg1	biofilms					518:525	S. mutans biofilms	508:525	S. mutans biofilms	508:525	Therefore, the present study investigated the impact of norspermidine on S. mutans biofilms.
27922663	2	18	from	formation	220:228	arg1	essential					199:207	essential	199:207	essential	199:207	Polyamines are essential in biofilm formation of certain bacteria.
27922663	6	19	from	architectures	542:554	arg1	groups					601:606	norspermidine and control groups	575:606	groups	601:606	The different architectures of the biofilms in norspermidine and control groups indicated that the basic units, bacteria‑exopolysaccharide units (BEUs), represent the exopolysaccharide (EPS) and bacterial assembly pattern in S. mutans biofilms.
27922663	9	20	theme	elements	1245:1252	arg1	downregulation					1227:1240	the downregulation	1223:1240	the downregulation of elements of the quorum‑sensing system	1223:1281	Gene‑expression microarray analysis indicated that the formation of an irregular architecture in the norspermidine group was potentially attributable to the downregulation of elements of the quorum‑sensing system (by 2.7‑15‑fold).
27922663	5	21	theme	mutans	511:516	arg1	biofilms					518:525	S. mutans biofilms	508:525	S. mutans biofilms	508:525	Therefore, the present study investigated the impact of norspermidine on S. mutans biofilms.
27922663	6	22	theme	basic	627:631	arg1	units					633:637	the basic units	623:637	the basic units	623:637	The different architectures of the biofilms in norspermidine and control groups indicated that the basic units, bacteria‑exopolysaccharide units (BEUs), represent the exopolysaccharide (EPS) and bacterial assembly pattern in S. mutans biofilms.
27922663	6	22	theme	basic	627:631	arg1	units					667:671	bacteria‑exopolysaccharide units	640:671	bacteria‑exopolysaccharide units (BEUs)	640:678	The different architectures of the biofilms in norspermidine and control groups indicated that the basic units, bacteria‑exopolysaccharide units (BEUs), represent the exopolysaccharide (EPS) and bacterial assembly pattern in S. mutans biofilms.
27922663	9	23	dep	attributable	1207:1218	arg1	2.7‑15‑fold					1287:1297	2.7‑15‑fold	1287:1297	2.7‑15‑fold	1287:1297	Gene‑expression microarray analysis indicated that the formation of an irregular architecture in the norspermidine group was potentially attributable to the downregulation of elements of the quorum‑sensing system (by 2.7‑15‑fold).
27922663	11	24	theme	S.	1520:1521	arg1	system					1545:1550	S. mutans quorum-sensing system	1520:1550	S. mutans quorum-sensing system	1520:1550	Norspermidine can lead to structure change in BEUs by influencing S. mutans quorum-sensing system.
27922663	9	25	from	group	1185:1189	arg1	formation					1125:1133	the formation	1121:1133	the formation of an irregular architecture in the norspermidine group	1121:1189	Gene‑expression microarray analysis indicated that the formation of an irregular architecture in the norspermidine group was potentially attributable to the downregulation of elements of the quorum‑sensing system (by 2.7‑15‑fold).
27922663	9	25	from	group	1185:1189	arg1	attributable					1207:1218	attributable	1207:1218	attributable	1207:1218	Gene‑expression microarray analysis indicated that the formation of an irregular architecture in the norspermidine group was potentially attributable to the downregulation of elements of the quorum‑sensing system (by 2.7‑15‑fold).
27922663	7	26	theme	biofilm	820:826	arg1	formation					828:836	S. mutans biofilm formation	810:836	S. mutans biofilm formation	810:836	In addition, norspermidine inhibited S. mutans biofilm formation and changed the basic composition of the biofilm, which led to an unusual EPS architecture.
27922663	8	27	theme	viability	1024:1032	arg1	rate					1011:1014	the rate	1007:1014	the rate of cell viability	1007:1032	Therefore, 5 mM norspermidine inhibited biofilm formation both by decreasing the rate of cell viability and changing the biofilm structure.
27922663	8	28	theme	biofilm	970:976	arg1	formation					978:986	biofilm formation	970:986	biofilm formation	970:986	Therefore, 5 mM norspermidine inhibited biofilm formation both by decreasing the rate of cell viability and changing the biofilm structure.
27922663	10	29	theme	biofilm	1378:1384	arg1	structure					1355:1363	a basic structure	1347:1363	a basic structure of S. mutans biofilm	1347:1384	The present study suggested that the BEUs are a basic structure of S. mutans biofilm and its assembly is regulated majorly by the quorum‑sensing system.
27922663	10	29	theme	biofilm	1378:1384	arg1	BEUs					1338:1341	the BEUs	1334:1341	the BEUs	1334:1341	The present study suggested that the BEUs are a basic structure of S. mutans biofilm and its assembly is regulated majorly by the quorum‑sensing system.
27922663	0	30	theme	basic	26:30	arg1	structure					32:40	the basic structure	22:40	the basic structure of S. mutans biofilm	22:61	Norspermidine changes the basic structure of S. mutans biofilm.
27922663	10	31	theme	quorum‑sensing	1431:1444	arg1	system					1446:1451	the quorum‑sensing system	1427:1451	the quorum‑sensing system	1427:1451	The present study suggested that the BEUs are a basic structure of S. mutans biofilm and its assembly is regulated majorly by the quorum‑sensing system.
27922663	4	32	theme	norspermidine	382:394	arg1	role					374:377	the role	370:377	the role of norspermidine in S. mutans biofilms	370:416	However, the role of norspermidine in S. mutans biofilms remains unknown.
27922663	9	33	from	formation	1125:1133	arg1	group					1185:1189	the norspermidine group	1167:1189	the norspermidine group	1167:1189	Gene‑expression microarray analysis indicated that the formation of an irregular architecture in the norspermidine group was potentially attributable to the downregulation of elements of the quorum‑sensing system (by 2.7‑15‑fold).
27922663	7	34	theme	basic	854:858	arg1	composition					860:870	the basic composition	850:870	the basic composition of the biofilm, which led to an unusual EPS architecture	850:927	In addition, norspermidine inhibited S. mutans biofilm formation and changed the basic composition of the biofilm, which led to an unusual EPS architecture.
27922663	7	35	theme	S.	810:811	arg1	formation					828:836	S. mutans biofilm formation	810:836	S. mutans biofilm formation	810:836	In addition, norspermidine inhibited S. mutans biofilm formation and changed the basic composition of the biofilm, which led to an unusual EPS architecture.
27922663	9	36	theme	system	1276:1281	arg1	elements					1245:1252	elements	1245:1252	elements of the quorum‑sensing system	1245:1281	Gene‑expression microarray analysis indicated that the formation of an irregular architecture in the norspermidine group was potentially attributable to the downregulation of elements of the quorum‑sensing system (by 2.7‑15‑fold).
27922663	4	37	from	role	374:377	arg1	biofilms					409:416	S. mutans biofilms	399:416	S. mutans biofilms	399:416	However, the role of norspermidine in S. mutans biofilms remains unknown.
27922663	11	38	theme	structure	1480:1488	arg1	change					1490:1495	structure change	1480:1495	structure change in BEUs	1480:1503	Norspermidine can lead to structure change in BEUs by influencing S. mutans quorum-sensing system.
27922663	6	39	from	pattern	742:748	arg1	biofilms					763:770	S. mutans biofilms	753:770	S. mutans biofilms	753:770	The different architectures of the biofilms in norspermidine and control groups indicated that the basic units, bacteria‑exopolysaccharide units (BEUs), represent the exopolysaccharide (EPS) and bacterial assembly pattern in S. mutans biofilms.
27922663	7	40	theme	biofilm	879:885	arg1	composition					860:870	the basic composition	850:870	the basic composition of the biofilm, which led to an unusual EPS architecture	850:927	In addition, norspermidine inhibited S. mutans biofilm formation and changed the basic composition of the biofilm, which led to an unusual EPS architecture.
27922663	11	41	theme	quorum-sensing	1530:1543	arg1	system					1545:1550	S. mutans quorum-sensing system	1520:1550	S. mutans quorum-sensing system	1520:1550	Norspermidine can lead to structure change in BEUs by influencing S. mutans quorum-sensing system.
27922663	5	42	theme	norspermidine	491:503	arg1	impact					481:486	the impact	477:486	the impact of norspermidine on S. mutans biofilms	477:525	Therefore, the present study investigated the impact of norspermidine on S. mutans biofilms.
27922663	9	43	from	architecture	1151:1162	arg1	group					1185:1189	the norspermidine group	1167:1189	the norspermidine group	1167:1189	Gene‑expression microarray analysis indicated that the formation of an irregular architecture in the norspermidine group was potentially attributable to the downregulation of elements of the quorum‑sensing system (by 2.7‑15‑fold).
27922663	6	44	theme	control	593:599	arg1	groups					601:606	norspermidine and control groups	575:606	groups	601:606	The different architectures of the biofilms in norspermidine and control groups indicated that the basic units, bacteria‑exopolysaccharide units (BEUs), represent the exopolysaccharide (EPS) and bacterial assembly pattern in S. mutans biofilms.
27922663	11	45	from	change	1490:1495	arg1	BEUs					1500:1503	BEUs	1500:1503	BEUs	1500:1503	Norspermidine can lead to structure change in BEUs by influencing S. mutans quorum-sensing system.
27922663	9	46	theme	architecture	1151:1162	arg1	formation					1125:1133	the formation	1121:1133	the formation of an irregular architecture in the norspermidine group	1121:1189	Gene‑expression microarray analysis indicated that the formation of an irregular architecture in the norspermidine group was potentially attributable to the downregulation of elements of the quorum‑sensing system (by 2.7‑15‑fold).
27922663	9	46	theme	architecture	1151:1162	arg1	attributable					1207:1218	attributable	1207:1218	attributable	1207:1218	Gene‑expression microarray analysis indicated that the formation of an irregular architecture in the norspermidine group was potentially attributable to the downregulation of elements of the quorum‑sensing system (by 2.7‑15‑fold).
27922663	0	47	theme	biofilm	55:61	arg1	structure					32:40	the basic structure	22:40	the basic structure of S. mutans biofilm	22:61	Norspermidine changes the basic structure of S. mutans biofilm.
27922663	11	48	theme	mutans	1523:1528	arg1	system					1545:1550	S. mutans quorum-sensing system	1520:1550	S. mutans quorum-sensing system	1520:1550	Norspermidine can lead to structure change in BEUs by influencing S. mutans quorum-sensing system.
27922663	2	49	theme	bacteria	241:248	arg1	formation					220:228	biofilm formation	212:228	biofilm formation of certain bacteria	212:248	Polyamines are essential in biofilm formation of certain bacteria.
27922663	9	50	theme	quorum‑sensing	1261:1274	arg1	system					1276:1281	the quorum‑sensing system	1257:1281	the quorum‑sensing system	1257:1281	Gene‑expression microarray analysis indicated that the formation of an irregular architecture in the norspermidine group was potentially attributable to the downregulation of elements of the quorum‑sensing system (by 2.7‑15‑fold).
27922663	1	51	theme	three-dimensional	107:123	arg1	structure					125:133	the three-dimensional structure	103:133	the three-dimensional structure of Streptococcus mutans biofilms	103:166	The factors regulating the assembly of the three-dimensional structure of Streptococcus mutans biofilms remain obscure.
27922663	0	52	theme	S. mutans	45:53	arg1	biofilm					55:61	S. mutans biofilm	45:61	S. mutans biofilm	45:61	Norspermidine changes the basic structure of S. mutans biofilm.
27922663	2	53	theme	certain	233:239	arg1	bacteria					241:248	certain bacteria	233:248	certain bacteria	233:248	Polyamines are essential in biofilm formation of certain bacteria.
27922663	8	54	theme	5 mM	941:944	arg1	norspermidine					946:958	5 mM norspermidine	941:958	5 mM norspermidine	941:958	Therefore, 5 mM norspermidine inhibited biofilm formation both by decreasing the rate of cell viability and changing the biofilm structure.
27922663	1	55	theme	structure	125:133	arg1	assembly					91:98	the assembly	87:98	the assembly of the three-dimensional structure of Streptococcus mutans biofilms	87:166	The factors regulating the assembly of the three-dimensional structure of Streptococcus mutans biofilms remain obscure.
27922663	7	56	theme	EPS	912:914	arg1	architecture					916:927	an unusual EPS architecture	901:927	an unusual EPS architecture	901:927	In addition, norspermidine inhibited S. mutans biofilm formation and changed the basic composition of the biofilm, which led to an unusual EPS architecture.
27922663	8	57	theme	cell	1019:1022	arg1	viability					1024:1032	cell viability	1019:1032	cell viability	1019:1032	Therefore, 5 mM norspermidine inhibited biofilm formation both by decreasing the rate of cell viability and changing the biofilm structure.
27922663	3	58	theme	controversial	299:311	arg1	polyamine					313:321	a controversial polyamine	297:321	a controversial polyamine that can lead to biofilm disassembly	297:358	Norspermidine, an unusual polyamine, has been a controversial polyamine that can lead to biofilm disassembly.
27922663	3	58	theme	controversial	299:311	arg1	Norspermidine					251:263	Norspermidine	251:263	Norspermidine	251:263	Norspermidine, an unusual polyamine, has been a controversial polyamine that can lead to biofilm disassembly.
27922663	6	59	theme	mutans	756:761	arg1	biofilms					763:770	S. mutans biofilms	753:770	S. mutans biofilms	753:770	The different architectures of the biofilms in norspermidine and control groups indicated that the basic units, bacteria‑exopolysaccharide units (BEUs), represent the exopolysaccharide (EPS) and bacterial assembly pattern in S. mutans biofilms.
27922663	5	60	theme	present	450:456	arg1	study					458:462	the present study	446:462	the present study	446:462	Therefore, the present study investigated the impact of norspermidine on S. mutans biofilms.
27922663	9	61	theme	norspermidine	1171:1183	arg1	group					1185:1189	the norspermidine group	1167:1189	the norspermidine group	1167:1189	Gene‑expression microarray analysis indicated that the formation of an irregular architecture in the norspermidine group was potentially attributable to the downregulation of elements of the quorum‑sensing system (by 2.7‑15‑fold).
27922663	2	62	theme	biofilm	212:218	arg1	formation					220:228	biofilm formation	212:228	biofilm formation of certain bacteria	212:248	Polyamines are essential in biofilm formation of certain bacteria.
27922663	10	63	theme	mutans	1371:1376	arg1	biofilm					1378:1384	S. mutans biofilm	1368:1384	S. mutans biofilm	1368:1384	The present study suggested that the BEUs are a basic structure of S. mutans biofilm and its assembly is regulated majorly by the quorum‑sensing system.
27922663	8	64	dep	decreasing	996:1005	arg1	both					988:991	both	988:991	both	988:991	Therefore, 5 mM norspermidine inhibited biofilm formation both by decreasing the rate of cell viability and changing the biofilm structure.
27922663	8	65	dep	inhibited	960:968	arg1	decreasing					996:1005	decreasing	996:1005	decreasing the rate of cell viability	996:1032	Therefore, 5 mM norspermidine inhibited biofilm formation both by decreasing the rate of cell viability and changing the biofilm structure.
27922663	8	65	dep	inhibited	960:968	arg1	changing					1038:1045	changing	1038:1045	changing the biofilm structure	1038:1067	Therefore, 5 mM norspermidine inhibited biofilm formation both by decreasing the rate of cell viability and changing the biofilm structure.
27922663	3	66	theme	biofilm	340:346	arg1	disassembly					348:358	biofilm disassembly	340:358	biofilm disassembly	340:358	Norspermidine, an unusual polyamine, has been a controversial polyamine that can lead to biofilm disassembly.
27922663	9	67	theme	microarray	1086:1095	arg1	analysis					1097:1104	Gene‑expression microarray analysis	1070:1104	Gene‑expression microarray analysis	1070:1104	Gene‑expression microarray analysis indicated that the formation of an irregular architecture in the norspermidine group was potentially attributable to the downregulation of elements of the quorum‑sensing system (by 2.7‑15‑fold).
27922663	10	68	theme	present	1305:1311	arg1	study					1313:1317	The present study	1301:1317	The present study	1301:1317	The present study suggested that the BEUs are a basic structure of S. mutans biofilm and its assembly is regulated majorly by the quorum‑sensing system.
27922663	1	69	theme	mutans	152:157	arg1	biofilms					159:166	Streptococcus mutans biofilms	138:166	Streptococcus mutans biofilms	138:166	The factors regulating the assembly of the three-dimensional structure of Streptococcus mutans biofilms remain obscure.
27922663	7	70	theme	unusual	904:910	arg1	architecture					916:927	an unusual EPS architecture	901:927	an unusual EPS architecture	901:927	In addition, norspermidine inhibited S. mutans biofilm formation and changed the basic composition of the biofilm, which led to an unusual EPS architecture.
27922663	6	71	theme	biofilms	563:570	arg1	architectures					542:554	The different architectures	528:554	The different architectures of the biofilms in norspermidine and control groups	528:606	The different architectures of the biofilms in norspermidine and control groups indicated that the basic units, bacteria‑exopolysaccharide units (BEUs), represent the exopolysaccharide (EPS) and bacterial assembly pattern in S. mutans biofilms.
27922663	7	72	dep	S.	810:811	arg1	mutans					813:818	mutans	813:818	mutans	813:818	In addition, norspermidine inhibited S. mutans biofilm formation and changed the basic composition of the biofilm, which led to an unusual EPS architecture.
27922663	1	73	theme	biofilms	159:166	arg1	structure					125:133	the three-dimensional structure	103:133	the three-dimensional structure of Streptococcus mutans biofilms	103:166	The factors regulating the assembly of the three-dimensional structure of Streptococcus mutans biofilms remain obscure.
27922663	5	74	from	impact	481:486	arg1	biofilms					518:525	S. mutans biofilms	508:525	S. mutans biofilms	508:525	Therefore, the present study investigated the impact of norspermidine on S. mutans biofilms.
27643998	0	0	theme	Hydrogels	115:123	arg1	Development					63:73	the Development	59:73	the Development of Self-Healing and Bioactive Composite Hydrogels	59:123	Exploiting Bisphosphonate-Bioactive-Glass Interactions for the Development of Self-Healing and Bioactive Composite Hydrogels.
27643998	7	1	theme	composite	1239:1247	arg1	hydrogels					1249:1257	injectable, bioactive and self-healing organic-inorganic composite hydrogels	1182:1257	injectable, bioactive and self-healing organic-inorganic composite hydrogels	1182:1257	Following this approach, injectable, bioactive and self-healing organic-inorganic composite hydrogels are produced, which mineralize abundantly and rapidly in simulated body fluid.
27643998	1	2	theme	biomedical	194:203	arg1	engineering					234:244	tissue engineering	227:244	tissue engineering	227:244	Hydrogels are widely recognized as promising candidates for various biomedical applications, such as tissue engineering.
27643998	1	2	theme	biomedical	194:203	arg1	applications					205:216	various biomedical applications	186:216	various biomedical applications	186:216	Hydrogels are widely recognized as promising candidates for various biomedical applications, such as tissue engineering.
27643998	0	3	theme	Composite	105:113	arg1	Hydrogels					115:123	Self-Healing and Bioactive Composite Hydrogels	78:123	Self-Healing and Bioactive Composite Hydrogels	78:123	Exploiting Bisphosphonate-Bioactive-Glass Interactions for the Development of Self-Healing and Bioactive Composite Hydrogels.
27643998	7	4	theme	organic-inorganic	1221:1237	arg1	hydrogels					1249:1257	injectable, bioactive and self-healing organic-inorganic composite hydrogels	1182:1257	injectable, bioactive and self-healing organic-inorganic composite hydrogels	1182:1257	Following this approach, injectable, bioactive and self-healing organic-inorganic composite hydrogels are produced, which mineralize abundantly and rapidly in simulated body fluid.
27643998	4	5	theme	45S5	740:743	arg1	Bioglass					745:752	45S5 Bioglass	740:752	45S5 Bioglass	740:752	In this study, the binding affinity of a bisphosphonate-functionalized polymer, hyaluronan, toward a bioactive glass (i.e., 45S5 Bioglass) is evaluated using force-distance measurements with atomic force microscopy.
27643998	4	5	theme	45S5	740:743	arg1	glass					727:731	a bioactive glass	715:731	a bioactive glass (i.e., 45S5 Bioglass)	715:753	In this study, the binding affinity of a bisphosphonate-functionalized polymer, hyaluronan, toward a bioactive glass (i.e., 45S5 Bioglass) is evaluated using force-distance measurements with atomic force microscopy.
27643998	7	6	theme	body	1326:1329	arg1	fluid					1331:1335	simulated body fluid	1316:1335	simulated body fluid	1316:1335	Following this approach, injectable, bioactive and self-healing organic-inorganic composite hydrogels are produced, which mineralize abundantly and rapidly in simulated body fluid.
27643998	7	7	theme	self-healing	1208:1219	arg1	hydrogels					1249:1257	injectable, bioactive and self-healing organic-inorganic composite hydrogels	1182:1257	injectable, bioactive and self-healing organic-inorganic composite hydrogels	1182:1257	Following this approach, injectable, bioactive and self-healing organic-inorganic composite hydrogels are produced, which mineralize abundantly and rapidly in simulated body fluid.
27643998	2	8	theme	biological	328:337	arg1	performance					354:364	the biological and mechanical performance	324:364	the biological and mechanical performance of hydrogel systems	324:384	Recently, extensive research efforts have been devoted to the improvement of the biological and mechanical performance of hydrogel systems by incorporation of functional groups and/or inorganic particles in their composition.
27643998	5	9	theme	materials	1020:1028	arg1	ability					1003:1009	the viscoelastic and self-healing ability	969:1009	the viscoelastic and self-healing ability of these materials	969:1028	The strong interaction between bisphosphonate and bioactive glass is then exploited to develop organic-inorganic composite hydrogels and the viscoelastic and self-healing ability of these materials are investigated.
27643998	4	10	theme	force	814:818	arg1	microscopy					820:829	atomic force microscopy	807:829	atomic force microscopy	807:829	In this study, the binding affinity of a bisphosphonate-functionalized polymer, hyaluronan, toward a bioactive glass (i.e., 45S5 Bioglass) is evaluated using force-distance measurements with atomic force microscopy.
27643998	2	11	from	incorporation	389:401	arg1	composition					460:470	their composition	454:470	their composition	454:470	Recently, extensive research efforts have been devoted to the improvement of the biological and mechanical performance of hydrogel systems by incorporation of functional groups and/or inorganic particles in their composition.
27643998	2	12	theme	functional	406:415	arg1	groups					417:422	functional groups	406:422	functional groups	406:422	Recently, extensive research efforts have been devoted to the improvement of the biological and mechanical performance of hydrogel systems by incorporation of functional groups and/or inorganic particles in their composition.
27643998	4	13	theme	binding	635:641	arg1	affinity					643:650	the binding affinity	631:650	the binding affinity of a bisphosphonate-functionalized polymer, hyaluronan, toward a bioactive glass (i.e., 45S5 Bioglass)	631:753	In this study, the binding affinity of a bisphosphonate-functionalized polymer, hyaluronan, toward a bioactive glass (i.e., 45S5 Bioglass) is evaluated using force-distance measurements with atomic force microscopy.
27643998	7	14	theme	bioactive	1194:1202	arg1	hydrogels					1249:1257	injectable, bioactive and self-healing organic-inorganic composite hydrogels	1182:1257	injectable, bioactive and self-healing organic-inorganic composite hydrogels	1182:1257	Following this approach, injectable, bioactive and self-healing organic-inorganic composite hydrogels are produced, which mineralize abundantly and rapidly in simulated body fluid.
27643998	3	15	theme	binding	579:585	arg1	affinity					587:594	a strong binding affinity	570:594	a strong binding affinity for hydroxyapatite	570:613	Bisphosphonates are a class of drugs, commonly used for treatment of osteoporosis, which exhibit a strong binding affinity for hydroxyapatite.
27643998	4	16	theme	bioactive	717:725	arg1	Bioglass					745:752	45S5 Bioglass	740:752	45S5 Bioglass	740:752	In this study, the binding affinity of a bisphosphonate-functionalized polymer, hyaluronan, toward a bioactive glass (i.e., 45S5 Bioglass) is evaluated using force-distance measurements with atomic force microscopy.
27643998	4	16	theme	bioactive	717:725	arg1	glass					727:731	a bioactive glass	715:731	a bioactive glass (i.e., 45S5 Bioglass)	715:753	In this study, the binding affinity of a bisphosphonate-functionalized polymer, hyaluronan, toward a bioactive glass (i.e., 45S5 Bioglass) is evaluated using force-distance measurements with atomic force microscopy.
27643998	7	17	theme	simulated	1316:1324	arg1	fluid					1331:1335	simulated body fluid	1316:1335	simulated body fluid	1316:1335	Following this approach, injectable, bioactive and self-healing organic-inorganic composite hydrogels are produced, which mineralize abundantly and rapidly in simulated body fluid.
27643998	3	18	theme	drugs	504:508	arg1	Bisphosphonates					473:487	Bisphosphonates	473:487	Bisphosphonates	473:487	Bisphosphonates are a class of drugs, commonly used for treatment of osteoporosis, which exhibit a strong binding affinity for hydroxyapatite.
27643998	3	18	theme	drugs	504:508	arg1	class					495:499	a class	493:499	a class	493:499	Bisphosphonates are a class of drugs, commonly used for treatment of osteoporosis, which exhibit a strong binding affinity for hydroxyapatite.
27643998	0	19	theme	Bisphosphonate-Bioactive-Glass	11:40	arg1	Interactions					42:53	Bisphosphonate-Bioactive-Glass Interactions	11:53	Bisphosphonate-Bioactive-Glass Interactions	11:53	Exploiting Bisphosphonate-Bioactive-Glass Interactions for the Development of Self-Healing and Bioactive Composite Hydrogels.
27643998	3	20	theme	strong	572:577	arg1	affinity					587:594	a strong binding affinity	570:594	a strong binding affinity for hydroxyapatite	570:613	Bisphosphonates are a class of drugs, commonly used for treatment of osteoporosis, which exhibit a strong binding affinity for hydroxyapatite.
27643998	7	21	theme	injectable	1182:1191	arg1	hydrogels					1249:1257	injectable, bioactive and self-healing organic-inorganic composite hydrogels	1182:1257	injectable, bioactive and self-healing organic-inorganic composite hydrogels	1182:1257	Following this approach, injectable, bioactive and self-healing organic-inorganic composite hydrogels are produced, which mineralize abundantly and rapidly in simulated body fluid.
27643998	2	22	theme	particles	441:449	arg1	incorporation					389:401	incorporation	389:401	incorporation of functional groups and/or inorganic particles in their composition	389:470	Recently, extensive research efforts have been devoted to the improvement of the biological and mechanical performance of hydrogel systems by incorporation of functional groups and/or inorganic particles in their composition.
27643998	5	23	theme	bioactive	882:890	arg1	glass					892:896	bioactive glass	882:896	bioactive glass	882:896	The strong interaction between bisphosphonate and bioactive glass is then exploited to develop organic-inorganic composite hydrogels and the viscoelastic and self-healing ability of these materials are investigated.
27643998	8	24	theme	composite	1368:1376	arg1	gels					1378:1381	these composite gels	1362:1381	these composite gels	1362:1381	These properties render these composite gels suitable for applications in bone-tissue engineering.
27643998	2	25	theme	performance	354:364	arg1	improvement					309:319	the improvement	305:319	the improvement of the biological and mechanical performance of hydrogel systems	305:384	Recently, extensive research efforts have been devoted to the improvement of the biological and mechanical performance of hydrogel systems by incorporation of functional groups and/or inorganic particles in their composition.
27643998	2	26	theme	inorganic	431:439	arg1	particles					441:449	inorganic particles	431:449	inorganic particles	431:449	Recently, extensive research efforts have been devoted to the improvement of the biological and mechanical performance of hydrogel systems by incorporation of functional groups and/or inorganic particles in their composition.
27643998	2	27	theme	extensive	257:265	arg1	efforts					276:282	extensive research efforts	257:282	extensive research efforts	257:282	Recently, extensive research efforts have been devoted to the improvement of the biological and mechanical performance of hydrogel systems by incorporation of functional groups and/or inorganic particles in their composition.
27643998	2	28	theme	mechanical	343:352	arg1	performance					354:364	the biological and mechanical performance	324:364	the biological and mechanical performance of hydrogel systems	324:384	Recently, extensive research efforts have been devoted to the improvement of the biological and mechanical performance of hydrogel systems by incorporation of functional groups and/or inorganic particles in their composition.
27643998	4	29	theme	force-distance	774:787	arg1	measurements					789:800	force-distance measurements	774:800	force-distance measurements	774:800	In this study, the binding affinity of a bisphosphonate-functionalized polymer, hyaluronan, toward a bioactive glass (i.e., 45S5 Bioglass) is evaluated using force-distance measurements with atomic force microscopy.
27643998	5	30	theme	self-healing	990:1001	arg1	ability					1003:1009	the viscoelastic and self-healing ability	969:1009	the viscoelastic and self-healing ability of these materials	969:1028	The strong interaction between bisphosphonate and bioactive glass is then exploited to develop organic-inorganic composite hydrogels and the viscoelastic and self-healing ability of these materials are investigated.
27643998	5	31	theme	strong	836:841	arg1	interaction					843:853	The strong interaction	832:853	The strong interaction between bisphosphonate and bioactive glass	832:896	The strong interaction between bisphosphonate and bioactive glass is then exploited to develop organic-inorganic composite hydrogels and the viscoelastic and self-healing ability of these materials are investigated.
27643998	8	32	theme	bone-tissue	1412:1422	arg1	engineering					1424:1434	bone-tissue engineering	1412:1434	bone-tissue engineering	1412:1434	These properties render these composite gels suitable for applications in bone-tissue engineering.
27643998	1	33	theme	tissue	227:232	arg1	engineering					234:244	tissue engineering	227:244	tissue engineering	227:244	Hydrogels are widely recognized as promising candidates for various biomedical applications, such as tissue engineering.
27643998	6	34	theme	body	1145:1148	arg1	fluid					1150:1154	simulated body fluid	1135:1154	simulated body fluid	1135:1154	Finally, the stability and mineralization behavior of these hydrogels are evaluated in simulated body fluid.
27643998	5	35	theme	organic-inorganic	927:943	arg1	hydrogels					955:963	organic-inorganic composite hydrogels	927:963	organic-inorganic composite hydrogels	927:963	The strong interaction between bisphosphonate and bioactive glass is then exploited to develop organic-inorganic composite hydrogels and the viscoelastic and self-healing ability of these materials are investigated.
27643998	6	36	theme	simulated	1135:1143	arg1	fluid					1150:1154	simulated body fluid	1135:1154	simulated body fluid	1135:1154	Finally, the stability and mineralization behavior of these hydrogels are evaluated in simulated body fluid.
27643998	0	37	theme	Self-Healing	78:89	arg1	Hydrogels					115:123	Self-Healing and Bioactive Composite Hydrogels	78:123	Self-Healing and Bioactive Composite Hydrogels	78:123	Exploiting Bisphosphonate-Bioactive-Glass Interactions for the Development of Self-Healing and Bioactive Composite Hydrogels.
27643998	2	38	theme	research	267:274	arg1	efforts					276:282	extensive research efforts	257:282	extensive research efforts	257:282	Recently, extensive research efforts have been devoted to the improvement of the biological and mechanical performance of hydrogel systems by incorporation of functional groups and/or inorganic particles in their composition.
27643998	3	39	theme	osteoporosis	542:553	arg1	treatment					529:537	treatment	529:537	treatment of osteoporosis	529:553	Bisphosphonates are a class of drugs, commonly used for treatment of osteoporosis, which exhibit a strong binding affinity for hydroxyapatite.
27643998	1	40	theme	various	186:192	arg1	engineering					234:244	tissue engineering	227:244	tissue engineering	227:244	Hydrogels are widely recognized as promising candidates for various biomedical applications, such as tissue engineering.
27643998	1	40	theme	various	186:192	arg1	applications					205:216	various biomedical applications	186:216	various biomedical applications	186:216	Hydrogels are widely recognized as promising candidates for various biomedical applications, such as tissue engineering.
27643998	1	41	theme	promising	161:169	arg1	Hydrogels					126:134	Hydrogels	126:134	Hydrogels	126:134	Hydrogels are widely recognized as promising candidates for various biomedical applications, such as tissue engineering.
27643998	1	41	theme	promising	161:169	arg1	candidates					171:180	promising candidates	161:180	promising candidates for various biomedical applications, such as tissue engineering	161:244	Hydrogels are widely recognized as promising candidates for various biomedical applications, such as tissue engineering.
27643998	4	42	theme	atomic	807:812	arg1	microscopy					820:829	atomic force microscopy	807:829	atomic force microscopy	807:829	In this study, the binding affinity of a bisphosphonate-functionalized polymer, hyaluronan, toward a bioactive glass (i.e., 45S5 Bioglass) is evaluated using force-distance measurements with atomic force microscopy.
27643998	4	43	dep	Bioglass	745:752	arg1	i.e.					734:737	i.e.	734:737	i.e.	734:737	In this study, the binding affinity of a bisphosphonate-functionalized polymer, hyaluronan, toward a bioactive glass (i.e., 45S5 Bioglass) is evaluated using force-distance measurements with atomic force microscopy.
27643998	2	44	theme	hydrogel	369:376	arg1	systems					378:384	hydrogel systems	369:384	hydrogel systems	369:384	Recently, extensive research efforts have been devoted to the improvement of the biological and mechanical performance of hydrogel systems by incorporation of functional groups and/or inorganic particles in their composition.
27643998	2	45	theme	systems	378:384	arg1	performance					354:364	the biological and mechanical performance	324:364	the biological and mechanical performance of hydrogel systems	324:384	Recently, extensive research efforts have been devoted to the improvement of the biological and mechanical performance of hydrogel systems by incorporation of functional groups and/or inorganic particles in their composition.
27643998	0	46	theme	Bioactive	95:103	arg1	Hydrogels					115:123	Self-Healing and Bioactive Composite Hydrogels	78:123	Self-Healing and Bioactive Composite Hydrogels	78:123	Exploiting Bisphosphonate-Bioactive-Glass Interactions for the Development of Self-Healing and Bioactive Composite Hydrogels.
27643998	6	47	dep	stability	1061:1069	arg1	the					1057:1059	the	1057:1059	the	1057:1059	Finally, the stability and mineralization behavior of these hydrogels are evaluated in simulated body fluid.
27643998	6	47	dep	stability	1061:1069	arg1	behavior					1090:1097	behavior	1090:1097	behavior	1090:1097	Finally, the stability and mineralization behavior of these hydrogels are evaluated in simulated body fluid.
27643998	5	48	theme	viscoelastic	973:984	arg1	ability					1003:1009	the viscoelastic and self-healing ability	969:1009	the viscoelastic and self-healing ability of these materials	969:1028	The strong interaction between bisphosphonate and bioactive glass is then exploited to develop organic-inorganic composite hydrogels and the viscoelastic and self-healing ability of these materials are investigated.
27643998	5	49	theme	composite	945:953	arg1	hydrogels					955:963	organic-inorganic composite hydrogels	927:963	organic-inorganic composite hydrogels	927:963	The strong interaction between bisphosphonate and bioactive glass is then exploited to develop organic-inorganic composite hydrogels and the viscoelastic and self-healing ability of these materials are investigated.
27643998	4	50	theme	polymer	687:693	arg1	affinity					643:650	the binding affinity	631:650	the binding affinity of a bisphosphonate-functionalized polymer, hyaluronan, toward a bioactive glass (i.e., 45S5 Bioglass)	631:753	In this study, the binding affinity of a bisphosphonate-functionalized polymer, hyaluronan, toward a bioactive glass (i.e., 45S5 Bioglass) is evaluated using force-distance measurements with atomic force microscopy.
27643998	6	51	theme	hydrogels	1108:1116	arg1	mineralization					1075:1088	mineralization	1075:1088	mineralization	1075:1088	Finally, the stability and mineralization behavior of these hydrogels are evaluated in simulated body fluid.
27643998	6	51	theme	hydrogels	1108:1116	arg1	stability					1061:1069	stability	1061:1069	stability	1061:1069	Finally, the stability and mineralization behavior of these hydrogels are evaluated in simulated body fluid.
27643998	8	52	from	applications	1396:1407	arg1	engineering					1424:1434	bone-tissue engineering	1412:1434	bone-tissue engineering	1412:1434	These properties render these composite gels suitable for applications in bone-tissue engineering.
27643998	2	53	theme	groups	417:422	arg1	incorporation					389:401	incorporation	389:401	incorporation of functional groups and/or inorganic particles in their composition	389:470	Recently, extensive research efforts have been devoted to the improvement of the biological and mechanical performance of hydrogel systems by incorporation of functional groups and/or inorganic particles in their composition.
27643998	4	54	theme	bisphosphonate-functionalized	657:685	arg1	hyaluronan					696:705	hyaluronan	696:705	hyaluronan	696:705	In this study, the binding affinity of a bisphosphonate-functionalized polymer, hyaluronan, toward a bioactive glass (i.e., 45S5 Bioglass) is evaluated using force-distance measurements with atomic force microscopy.
27643998	4	54	theme	bisphosphonate-functionalized	657:685	arg1	polymer					687:693	a bisphosphonate-functionalized polymer	655:693	a bisphosphonate-functionalized polymer	655:693	In this study, the binding affinity of a bisphosphonate-functionalized polymer, hyaluronan, toward a bioactive glass (i.e., 45S5 Bioglass) is evaluated using force-distance measurements with atomic force microscopy.
27261730	7	0	dep	nanocelluloses	1119:1132	arg1	nanocrystals					1145:1156	cellulose nanocrystals	1135:1156	cellulose nanocrystals	1135:1156	Moreover, we demonstrate for the first time that fully bio-based and transparent all-cellulose composites can be fabricated by simple aqueous blending of the obtained water-soluble CA and two kinds of nanocelluloses (cellulose nanocrystals and cellulose nanofibrils), which is attractive for the applications in disposable packaging materials, sheet coating and binders, etc.
27261730	7	0	dep	nanocelluloses	1119:1132	arg1	nanofibrils					1172:1182	cellulose nanofibrils	1162:1182	cellulose nanofibrils	1162:1182	Moreover, we demonstrate for the first time that fully bio-based and transparent all-cellulose composites can be fabricated by simple aqueous blending of the obtained water-soluble CA and two kinds of nanocelluloses (cellulose nanocrystals and cellulose nanofibrils), which is attractive for the applications in disposable packaging materials, sheet coating and binders, etc.
27261730	6	1	theme	original	903:910	arg1	WCFs					912:915	the original WCFs	899:915	the original WCFs	899:915	The structure of the obtained water-soluble CA was characterized and compared with the original WCFs.
27261730	2	2	theme	acetate	433:439	arg1	synthesis					410:418	controllable synthesis	397:418	controllable synthesis of cellulose acetate (CA) due to their dual function of swelling and catalyzing	397:498	Our previous study (Tian et al., 2014) demonstrated that acidic ionic liquids (ILs) can be highly efficient catalysts for controllable synthesis of cellulose acetate (CA) due to their dual function of swelling and catalyzing.
27261730	0	3	theme	composites	102:111	arg1	acetate					24:30	Water-soluble cellulose acetate	0:30	Water-soluble cellulose acetate from waste cotton fabrics	0:56	Water-soluble cellulose acetate from waste cotton fabrics and the aqueous processing of all-cellulose composites.
27261730	0	3	theme	composites	102:111	arg1	processing					74:83	the aqueous processing	62:83	the aqueous processing of all-cellulose composites	62:111	Water-soluble cellulose acetate from waste cotton fabrics and the aqueous processing of all-cellulose composites.
27261730	0	4	theme	all-cellulose	88:100	arg1	composites					102:111	all-cellulose composites	88:111	all-cellulose composites	88:111	Water-soluble cellulose acetate from waste cotton fabrics and the aqueous processing of all-cellulose composites.
27261730	3	5	theme	water-soluble	641:653	arg1	CA					655:656	water-soluble CA	641:656	water-soluble CA from WCFs	641:666	In this study, an optimized "quasi-homogeneous" process which required a small amount of acidic ILs as catalyst was developed to synthesize water-soluble CA from WCFs.
27261730	7	6	theme	aqueous	1052:1058	arg1	blending					1060:1067	simple aqueous blending	1045:1067	simple aqueous blending of the obtained water-soluble CA	1045:1100	Moreover, we demonstrate for the first time that fully bio-based and transparent all-cellulose composites can be fabricated by simple aqueous blending of the obtained water-soluble CA and two kinds of nanocelluloses (cellulose nanocrystals and cellulose nanofibrils), which is attractive for the applications in disposable packaging materials, sheet coating and binders, etc.
27261730	7	7	theme	CA	1099:1100	arg1	kinds					1110:1114	two kinds	1106:1114	two kinds of nanocelluloses (cellulose nanocrystals and cellulose nanofibrils), which is attractive for the applications in disposable packaging materials, sheet coating and binders, etc	1106:1291	Moreover, we demonstrate for the first time that fully bio-based and transparent all-cellulose composites can be fabricated by simple aqueous blending of the obtained water-soluble CA and two kinds of nanocelluloses (cellulose nanocrystals and cellulose nanofibrils), which is attractive for the applications in disposable packaging materials, sheet coating and binders, etc.
27261730	7	7	theme	CA	1099:1100	arg1	blending					1060:1067	simple aqueous blending	1045:1067	simple aqueous blending of the obtained water-soluble CA	1045:1100	Moreover, we demonstrate for the first time that fully bio-based and transparent all-cellulose composites can be fabricated by simple aqueous blending of the obtained water-soluble CA and two kinds of nanocelluloses (cellulose nanocrystals and cellulose nanofibrils), which is attractive for the applications in disposable packaging materials, sheet coating and binders, etc.
27261730	0	8	from	fabrics	50:56	arg1	acetate					24:30	Water-soluble cellulose acetate	0:30	Water-soluble cellulose acetate from waste cotton fabrics	0:56	Water-soluble cellulose acetate from waste cotton fabrics and the aqueous processing of all-cellulose composites.
27261730	0	8	from	fabrics	50:56	arg1	processing					74:83	the aqueous processing	62:83	the aqueous processing of all-cellulose composites	62:111	Water-soluble cellulose acetate from waste cotton fabrics and the aqueous processing of all-cellulose composites.
27261730	7	9	theme	transparent	987:997	arg1	composites					1013:1022	fully bio-based and transparent all-cellulose composites	967:1022	fully bio-based and transparent all-cellulose composites	967:1022	Moreover, we demonstrate for the first time that fully bio-based and transparent all-cellulose composites can be fabricated by simple aqueous blending of the obtained water-soluble CA and two kinds of nanocelluloses (cellulose nanocrystals and cellulose nanofibrils), which is attractive for the applications in disposable packaging materials, sheet coating and binders, etc.
27261730	5	10	from	WCFs	796:799	arg1	conversion					760:769	The highest conversion	748:769	The highest conversion of water-soluble CA from WCFs	748:799	The highest conversion of water-soluble CA from WCFs reached 90.8%.
27261730	5	10	from	WCFs	796:799	arg1	CA					788:789	water-soluble CA	774:789	water-soluble CA from WCFs	774:799	The highest conversion of water-soluble CA from WCFs reached 90.8%.
27261730	5	11	theme	water-soluble	774:786	arg1	CA					788:789	water-soluble CA	774:789	water-soluble CA from WCFs	774:799	The highest conversion of water-soluble CA from WCFs reached 90.8%.
27261730	7	12	theme	packaging	1241:1249	arg1	coating					1268:1274	sheet coating	1262:1274	sheet coating	1262:1274	Moreover, we demonstrate for the first time that fully bio-based and transparent all-cellulose composites can be fabricated by simple aqueous blending of the obtained water-soluble CA and two kinds of nanocelluloses (cellulose nanocrystals and cellulose nanofibrils), which is attractive for the applications in disposable packaging materials, sheet coating and binders, etc.
27261730	7	12	theme	packaging	1241:1249	arg1	binders					1280:1286	binders	1280:1286	binders	1280:1286	Moreover, we demonstrate for the first time that fully bio-based and transparent all-cellulose composites can be fabricated by simple aqueous blending of the obtained water-soluble CA and two kinds of nanocelluloses (cellulose nanocrystals and cellulose nanofibrils), which is attractive for the applications in disposable packaging materials, sheet coating and binders, etc.
27261730	7	12	theme	packaging	1241:1249	arg1	materials					1251:1259	disposable packaging materials	1230:1259	disposable packaging materials	1230:1259	Moreover, we demonstrate for the first time that fully bio-based and transparent all-cellulose composites can be fabricated by simple aqueous blending of the obtained water-soluble CA and two kinds of nanocelluloses (cellulose nanocrystals and cellulose nanofibrils), which is attractive for the applications in disposable packaging materials, sheet coating and binders, etc.
27261730	7	13	theme	simple	1045:1050	arg1	blending					1060:1067	simple aqueous blending	1045:1067	simple aqueous blending of the obtained water-soluble CA	1045:1100	Moreover, we demonstrate for the first time that fully bio-based and transparent all-cellulose composites can be fabricated by simple aqueous blending of the obtained water-soluble CA and two kinds of nanocelluloses (cellulose nanocrystals and cellulose nanofibrils), which is attractive for the applications in disposable packaging materials, sheet coating and binders, etc.
27261730	0	14	theme	cotton	43:48	arg1	fabrics					50:56	waste cotton fabrics	37:56	waste cotton fabrics	37:56	Water-soluble cellulose acetate from waste cotton fabrics and the aqueous processing of all-cellulose composites.
27261730	2	15	dep	demonstrated	314:325	arg1	Tian					295:298	Tian	295:298	Tian	295:298	Our previous study (Tian et al., 2014) demonstrated that acidic ionic liquids (ILs) can be highly efficient catalysts for controllable synthesis of cellulose acetate (CA) due to their dual function of swelling and catalyzing.
27261730	7	16	theme	water-soluble	1085:1097	arg1	CA					1099:1100	the obtained water-soluble CA	1072:1100	the obtained water-soluble CA	1072:1100	Moreover, we demonstrate for the first time that fully bio-based and transparent all-cellulose composites can be fabricated by simple aqueous blending of the obtained water-soluble CA and two kinds of nanocelluloses (cellulose nanocrystals and cellulose nanofibrils), which is attractive for the applications in disposable packaging materials, sheet coating and binders, etc.
27261730	0	17	theme	cellulose	14:22	arg1	acetate					24:30	Water-soluble cellulose acetate	0:30	Water-soluble cellulose acetate from waste cotton fabrics	0:56	Water-soluble cellulose acetate from waste cotton fabrics and the aqueous processing of all-cellulose composites.
27261730	7	18	theme	nanocelluloses	1119:1132	arg1	kinds					1110:1114	two kinds	1106:1114	two kinds of nanocelluloses (cellulose nanocrystals and cellulose nanofibrils), which is attractive for the applications in disposable packaging materials, sheet coating and binders, etc	1106:1291	Moreover, we demonstrate for the first time that fully bio-based and transparent all-cellulose composites can be fabricated by simple aqueous blending of the obtained water-soluble CA and two kinds of nanocelluloses (cellulose nanocrystals and cellulose nanofibrils), which is attractive for the applications in disposable packaging materials, sheet coating and binders, etc.
27261730	7	18	theme	nanocelluloses	1119:1132	arg1	blending					1060:1067	simple aqueous blending	1045:1067	simple aqueous blending of the obtained water-soluble CA	1045:1100	Moreover, we demonstrate for the first time that fully bio-based and transparent all-cellulose composites can be fabricated by simple aqueous blending of the obtained water-soluble CA and two kinds of nanocelluloses (cellulose nanocrystals and cellulose nanofibrils), which is attractive for the applications in disposable packaging materials, sheet coating and binders, etc.
27261730	7	19	theme	bio-based	973:981	arg1	composites					1013:1022	fully bio-based and transparent all-cellulose composites	967:1022	fully bio-based and transparent all-cellulose composites	967:1022	Moreover, we demonstrate for the first time that fully bio-based and transparent all-cellulose composites can be fabricated by simple aqueous blending of the obtained water-soluble CA and two kinds of nanocelluloses (cellulose nanocrystals and cellulose nanofibrils), which is attractive for the applications in disposable packaging materials, sheet coating and binders, etc.
27261730	2	20	theme	ionic	339:343	arg1	liquids					345:351	acidic ionic liquids	332:351	acidic ionic liquids (ILs)	332:357	Our previous study (Tian et al., 2014) demonstrated that acidic ionic liquids (ILs) can be highly efficient catalysts for controllable synthesis of cellulose acetate (CA) due to their dual function of swelling and catalyzing.
27261730	2	20	theme	ionic	339:343	arg1	ILs					354:356	ILs	354:356	ILs	354:356	Our previous study (Tian et al., 2014) demonstrated that acidic ionic liquids (ILs) can be highly efficient catalysts for controllable synthesis of cellulose acetate (CA) due to their dual function of swelling and catalyzing.
27261730	0	21	theme	Water-soluble	0:12	arg1	acetate					24:30	Water-soluble cellulose acetate	0:30	Water-soluble cellulose acetate from waste cotton fabrics	0:56	Water-soluble cellulose acetate from waste cotton fabrics and the aqueous processing of all-cellulose composites.
27261730	2	22	theme	acidic	332:337	arg1	liquids					345:351	acidic ionic liquids	332:351	acidic ionic liquids (ILs)	332:357	Our previous study (Tian et al., 2014) demonstrated that acidic ionic liquids (ILs) can be highly efficient catalysts for controllable synthesis of cellulose acetate (CA) due to their dual function of swelling and catalyzing.
27261730	2	22	theme	acidic	332:337	arg1	ILs					354:356	ILs	354:356	ILs	354:356	Our previous study (Tian et al., 2014) demonstrated that acidic ionic liquids (ILs) can be highly efficient catalysts for controllable synthesis of cellulose acetate (CA) due to their dual function of swelling and catalyzing.
27261730	2	23	theme	efficient	373:381	arg1	catalysts					383:391	highly efficient catalysts	366:391	highly efficient catalysts	366:391	Our previous study (Tian et al., 2014) demonstrated that acidic ionic liquids (ILs) can be highly efficient catalysts for controllable synthesis of cellulose acetate (CA) due to their dual function of swelling and catalyzing.
27261730	1	24	theme	value-added	253:263	arg1	products					265:272	value-added products	253:272	value-added products	253:272	The objective of this study is to explore the possibility of using waste cotton fabrics (WCFs) as low cost feedstock for the production of value-added products.
27261730	2	25	theme	previous	279:286	arg1	study					288:292	Our previous study	275:292	Our previous study	275:292	Our previous study (Tian et al., 2014) demonstrated that acidic ionic liquids (ILs) can be highly efficient catalysts for controllable synthesis of cellulose acetate (CA) due to their dual function of swelling and catalyzing.
27261730	7	26	theme	all-cellulose	999:1011	arg1	composites					1013:1022	fully bio-based and transparent all-cellulose composites	967:1022	fully bio-based and transparent all-cellulose composites	967:1022	Moreover, we demonstrate for the first time that fully bio-based and transparent all-cellulose composites can be fabricated by simple aqueous blending of the obtained water-soluble CA and two kinds of nanocelluloses (cellulose nanocrystals and cellulose nanofibrils), which is attractive for the applications in disposable packaging materials, sheet coating and binders, etc.
27261730	1	27	theme	cotton	187:192	arg1	fabrics					194:200	waste cotton fabrics	181:200	waste cotton fabrics (WCFs)	181:207	The objective of this study is to explore the possibility of using waste cotton fabrics (WCFs) as low cost feedstock for the production of value-added products.
27261730	1	27	theme	cotton	187:192	arg1	WCFs					203:206	WCFs	203:206	WCFs	203:206	The objective of this study is to explore the possibility of using waste cotton fabrics (WCFs) as low cost feedstock for the production of value-added products.
27261730	1	28	theme	products	265:272	arg1	production					239:248	the production	235:248	the production of value-added products	235:272	The objective of this study is to explore the possibility of using waste cotton fabrics (WCFs) as low cost feedstock for the production of value-added products.
27261730	2	29	theme	swelling	476:483	arg1	function					464:471	their dual function	453:471	their dual function of swelling and catalyzing	453:498	Our previous study (Tian et al., 2014) demonstrated that acidic ionic liquids (ILs) can be highly efficient catalysts for controllable synthesis of cellulose acetate (CA) due to their dual function of swelling and catalyzing.
27261730	2	30	theme	cellulose	423:431	arg1	CA					442:443	CA	442:443	CA	442:443	Our previous study (Tian et al., 2014) demonstrated that acidic ionic liquids (ILs) can be highly efficient catalysts for controllable synthesis of cellulose acetate (CA) due to their dual function of swelling and catalyzing.
27261730	2	30	theme	cellulose	423:431	arg1	acetate					433:439	cellulose acetate	423:439	cellulose acetate (CA)	423:444	Our previous study (Tian et al., 2014) demonstrated that acidic ionic liquids (ILs) can be highly efficient catalysts for controllable synthesis of cellulose acetate (CA) due to their dual function of swelling and catalyzing.
27261730	6	31	theme	CA	860:861	arg1	structure					820:828	The structure	816:828	The structure of the obtained water-soluble CA	816:861	The structure of the obtained water-soluble CA was characterized and compared with the original WCFs.
27261730	7	32	theme	first	951:955	arg1	time					957:960	the first time	947:960	the first time	947:960	Moreover, we demonstrate for the first time that fully bio-based and transparent all-cellulose composites can be fabricated by simple aqueous blending of the obtained water-soluble CA and two kinds of nanocelluloses (cellulose nanocrystals and cellulose nanofibrils), which is attractive for the applications in disposable packaging materials, sheet coating and binders, etc.
27261730	3	33	theme	small	574:578	arg1	amount					580:585	a small amount	572:585	a small amount of acidic ILs	572:599	In this study, an optimized "quasi-homogeneous" process which required a small amount of acidic ILs as catalyst was developed to synthesize water-soluble CA from WCFs.
27261730	3	33	theme	small	574:578	arg1	ILs					597:599	acidic ILs	590:599	acidic ILs	590:599	In this study, an optimized "quasi-homogeneous" process which required a small amount of acidic ILs as catalyst was developed to synthesize water-soluble CA from WCFs.
27261730	1	34	theme	waste	181:185	arg1	fabrics					194:200	waste cotton fabrics	181:200	waste cotton fabrics (WCFs)	181:207	The objective of this study is to explore the possibility of using waste cotton fabrics (WCFs) as low cost feedstock for the production of value-added products.
27261730	1	34	theme	waste	181:185	arg1	WCFs					203:206	WCFs	203:206	WCFs	203:206	The objective of this study is to explore the possibility of using waste cotton fabrics (WCFs) as low cost feedstock for the production of value-added products.
27261730	0	35	theme	waste	37:41	arg1	fabrics					50:56	waste cotton fabrics	37:56	waste cotton fabrics	37:56	Water-soluble cellulose acetate from waste cotton fabrics and the aqueous processing of all-cellulose composites.
27261730	6	36	theme	water-soluble	846:858	arg1	CA					860:861	the obtained water-soluble CA	833:861	the obtained water-soluble CA	833:861	The structure of the obtained water-soluble CA was characterized and compared with the original WCFs.
27261730	3	37	theme	optimized	519:527	arg1	process					549:555	an optimized "quasi-homogeneous" process	516:555	an optimized "quasi-homogeneous" process which required a small amount of acidic ILs as catalyst	516:611	In this study, an optimized "quasi-homogeneous" process which required a small amount of acidic ILs as catalyst was developed to synthesize water-soluble CA from WCFs.
27261730	5	38	theme	highest	752:758	arg1	conversion					760:769	The highest conversion	748:769	The highest conversion of water-soluble CA from WCFs	748:799	The highest conversion of water-soluble CA from WCFs reached 90.8%.
27261730	2	39	theme	dual	459:462	arg1	function					464:471	their dual function	453:471	their dual function of swelling and catalyzing	453:498	Our previous study (Tian et al., 2014) demonstrated that acidic ionic liquids (ILs) can be highly efficient catalysts for controllable synthesis of cellulose acetate (CA) due to their dual function of swelling and catalyzing.
27261730	4	40	theme	reaction	733:740	arg1	time					742:745	the reaction time	729:745	the reaction time	729:745	The process was optimized by varying the amounts of ILs and the reaction time.
27261730	2	41	theme	controllable	397:408	arg1	synthesis					410:418	controllable synthesis	397:418	controllable synthesis of cellulose acetate (CA) due to their dual function of swelling and catalyzing	397:498	Our previous study (Tian et al., 2014) demonstrated that acidic ionic liquids (ILs) can be highly efficient catalysts for controllable synthesis of cellulose acetate (CA) due to their dual function of swelling and catalyzing.
27261730	7	42	theme	disposable	1230:1239	arg1	coating					1268:1274	sheet coating	1262:1274	sheet coating	1262:1274	Moreover, we demonstrate for the first time that fully bio-based and transparent all-cellulose composites can be fabricated by simple aqueous blending of the obtained water-soluble CA and two kinds of nanocelluloses (cellulose nanocrystals and cellulose nanofibrils), which is attractive for the applications in disposable packaging materials, sheet coating and binders, etc.
27261730	7	42	theme	disposable	1230:1239	arg1	binders					1280:1286	binders	1280:1286	binders	1280:1286	Moreover, we demonstrate for the first time that fully bio-based and transparent all-cellulose composites can be fabricated by simple aqueous blending of the obtained water-soluble CA and two kinds of nanocelluloses (cellulose nanocrystals and cellulose nanofibrils), which is attractive for the applications in disposable packaging materials, sheet coating and binders, etc.
27261730	7	42	theme	disposable	1230:1239	arg1	materials					1251:1259	disposable packaging materials	1230:1259	disposable packaging materials	1230:1259	Moreover, we demonstrate for the first time that fully bio-based and transparent all-cellulose composites can be fabricated by simple aqueous blending of the obtained water-soluble CA and two kinds of nanocelluloses (cellulose nanocrystals and cellulose nanofibrils), which is attractive for the applications in disposable packaging materials, sheet coating and binders, etc.
27261730	3	43	theme	acidic	590:595	arg1	ILs					597:599	acidic ILs	590:599	acidic ILs	590:599	In this study, an optimized "quasi-homogeneous" process which required a small amount of acidic ILs as catalyst was developed to synthesize water-soluble CA from WCFs.
27261730	3	44	from	WCFs	663:666	arg1	CA					655:656	water-soluble CA	641:656	water-soluble CA from WCFs	641:666	In this study, an optimized "quasi-homogeneous" process which required a small amount of acidic ILs as catalyst was developed to synthesize water-soluble CA from WCFs.
27261730	7	45	theme	sheet	1262:1266	arg1	coating					1268:1274	sheet coating	1262:1274	sheet coating	1262:1274	Moreover, we demonstrate for the first time that fully bio-based and transparent all-cellulose composites can be fabricated by simple aqueous blending of the obtained water-soluble CA and two kinds of nanocelluloses (cellulose nanocrystals and cellulose nanofibrils), which is attractive for the applications in disposable packaging materials, sheet coating and binders, etc.
27261730	7	45	theme	sheet	1262:1266	arg1	materials					1251:1259	disposable packaging materials	1230:1259	disposable packaging materials	1230:1259	Moreover, we demonstrate for the first time that fully bio-based and transparent all-cellulose composites can be fabricated by simple aqueous blending of the obtained water-soluble CA and two kinds of nanocelluloses (cellulose nanocrystals and cellulose nanofibrils), which is attractive for the applications in disposable packaging materials, sheet coating and binders, etc.
27261730	3	46	theme	ILs	597:599	arg1	amount					580:585	a small amount	572:585	a small amount of acidic ILs	572:599	In this study, an optimized "quasi-homogeneous" process which required a small amount of acidic ILs as catalyst was developed to synthesize water-soluble CA from WCFs.
27261730	3	46	theme	ILs	597:599	arg1	ILs					597:599	acidic ILs	590:599	acidic ILs	590:599	In this study, an optimized "quasi-homogeneous" process which required a small amount of acidic ILs as catalyst was developed to synthesize water-soluble CA from WCFs.
27261730	3	47	theme	"	547:547	arg1	process					549:555	an optimized "quasi-homogeneous" process	516:555	an optimized "quasi-homogeneous" process which required a small amount of acidic ILs as catalyst	516:611	In this study, an optimized "quasi-homogeneous" process which required a small amount of acidic ILs as catalyst was developed to synthesize water-soluble CA from WCFs.
27261730	4	48	theme	ILs	721:723	arg1	amounts					710:716	the amounts	706:716	the amounts of ILs	706:723	The process was optimized by varying the amounts of ILs and the reaction time.
27261730	4	48	theme	ILs	721:723	arg1	time					742:745	the reaction time	729:745	the reaction time	729:745	The process was optimized by varying the amounts of ILs and the reaction time.
27261730	4	48	theme	ILs	721:723	arg1	ILs					721:723	ILs	721:723	ILs	721:723	The process was optimized by varying the amounts of ILs and the reaction time.
27261730	7	49	from	applications	1214:1225	arg1	coating					1268:1274	sheet coating	1262:1274	sheet coating	1262:1274	Moreover, we demonstrate for the first time that fully bio-based and transparent all-cellulose composites can be fabricated by simple aqueous blending of the obtained water-soluble CA and two kinds of nanocelluloses (cellulose nanocrystals and cellulose nanofibrils), which is attractive for the applications in disposable packaging materials, sheet coating and binders, etc.
27261730	7	49	from	applications	1214:1225	arg1	binders					1280:1286	binders	1280:1286	binders	1280:1286	Moreover, we demonstrate for the first time that fully bio-based and transparent all-cellulose composites can be fabricated by simple aqueous blending of the obtained water-soluble CA and two kinds of nanocelluloses (cellulose nanocrystals and cellulose nanofibrils), which is attractive for the applications in disposable packaging materials, sheet coating and binders, etc.
27261730	7	49	from	applications	1214:1225	arg1	materials					1251:1259	disposable packaging materials	1230:1259	disposable packaging materials	1230:1259	Moreover, we demonstrate for the first time that fully bio-based and transparent all-cellulose composites can be fabricated by simple aqueous blending of the obtained water-soluble CA and two kinds of nanocelluloses (cellulose nanocrystals and cellulose nanofibrils), which is attractive for the applications in disposable packaging materials, sheet coating and binders, etc.
27261730	5	50	theme	CA	788:789	arg1	conversion					760:769	The highest conversion	748:769	The highest conversion of water-soluble CA from WCFs	748:799	The highest conversion of water-soluble CA from WCFs reached 90.8%.
27261730	2	51	theme	catalyzing	489:498	arg1	function					464:471	their dual function	453:471	their dual function of swelling and catalyzing	453:498	Our previous study (Tian et al., 2014) demonstrated that acidic ionic liquids (ILs) can be highly efficient catalysts for controllable synthesis of cellulose acetate (CA) due to their dual function of swelling and catalyzing.
27261730	1	52	theme	study	136:140	arg1	objective					118:126	The objective	114:126	The objective of this study	114:140	The objective of this study is to explore the possibility of using waste cotton fabrics (WCFs) as low cost feedstock for the production of value-added products.
27261730	0	53	theme	aqueous	66:72	arg1	processing					74:83	the aqueous processing	62:83	the aqueous processing of all-cellulose composites	62:111	Water-soluble cellulose acetate from waste cotton fabrics and the aqueous processing of all-cellulose composites.
27261730	7	54	theme	cellulose	1162:1170	arg1	nanofibrils					1172:1182	cellulose nanofibrils	1162:1182	cellulose nanofibrils	1162:1182	Moreover, we demonstrate for the first time that fully bio-based and transparent all-cellulose composites can be fabricated by simple aqueous blending of the obtained water-soluble CA and two kinds of nanocelluloses (cellulose nanocrystals and cellulose nanofibrils), which is attractive for the applications in disposable packaging materials, sheet coating and binders, etc.
27261730	1	55	theme	low	212:214	arg1	feedstock					221:229	low cost feedstock	212:229	low cost feedstock for the production of value-added products	212:272	The objective of this study is to explore the possibility of using waste cotton fabrics (WCFs) as low cost feedstock for the production of value-added products.
27261730	7	56	theme	cellulose	1135:1143	arg1	nanocrystals					1145:1156	cellulose nanocrystals	1135:1156	cellulose nanocrystals	1135:1156	Moreover, we demonstrate for the first time that fully bio-based and transparent all-cellulose composites can be fabricated by simple aqueous blending of the obtained water-soluble CA and two kinds of nanocelluloses (cellulose nanocrystals and cellulose nanofibrils), which is attractive for the applications in disposable packaging materials, sheet coating and binders, etc.
27261730	3	57	theme	quasi-homogeneous	530:546	arg1	process					549:555	an optimized "quasi-homogeneous" process	516:555	an optimized "quasi-homogeneous" process which required a small amount of acidic ILs as catalyst	516:611	In this study, an optimized "quasi-homogeneous" process which required a small amount of acidic ILs as catalyst was developed to synthesize water-soluble CA from WCFs.
27261730	5	58	from	conversion	760:769	arg1	WCFs					796:799	WCFs	796:799	WCFs	796:799	The highest conversion of water-soluble CA from WCFs reached 90.8%.
27261730	7	59	theme	obtained	1076:1083	arg1	CA					1099:1100	the obtained water-soluble CA	1072:1100	the obtained water-soluble CA	1072:1100	Moreover, we demonstrate for the first time that fully bio-based and transparent all-cellulose composites can be fabricated by simple aqueous blending of the obtained water-soluble CA and two kinds of nanocelluloses (cellulose nanocrystals and cellulose nanofibrils), which is attractive for the applications in disposable packaging materials, sheet coating and binders, etc.
27261730	6	60	theme	obtained	837:844	arg1	CA					860:861	the obtained water-soluble CA	833:861	the obtained water-soluble CA	833:861	The structure of the obtained water-soluble CA was characterized and compared with the original WCFs.
27261730	1	61	theme	cost	216:219	arg1	feedstock					221:229	low cost feedstock	212:229	low cost feedstock for the production of value-added products	212:272	The objective of this study is to explore the possibility of using waste cotton fabrics (WCFs) as low cost feedstock for the production of value-added products.
25182372	2	0	theme	surface	455:461	arg1	properties					482:491	their mechanical, surface and microstructure properties	437:491	properties	482:491	METHODS Two alginate, three hyaluronic-based (Corgel™) hydrogel formulations and Matrigel were characterized for their mechanical, surface and microstructure properties using rheology, X-ray photoelectron spectroscopy and scanning electron microscopy, respectively.
25182372	1	1	theme	metabolic	295:303	arg1	activity					305:312	metabolic activity	295:312	metabolic activity	295:312	OBJECTIVE The goal of the present work was to evaluate in vitro and in vivo the influence of various types and compositions of natural hydrogels on the viability and metabolic activity of SCAPs.
25182372	6	2	theme	TUNEL	954:958	arg1	test					960:963	a TUNEL test	952:963	a TUNEL test	952:963	In vivo cell apoptosis was evaluated by a TUNEL test and RP89 cells were identified by human mitochondria immunostaining.
25182372	4	3	theme	treated	763:769	arg1	mice					771:774	cyclosporine treated mice	750:774	cyclosporine treated mice	750:774	Cells were cultured in vitro, or implanted in cyclosporine treated mice.
25182372	6	4	theme	RP89	969:972	arg1	cells					974:978	RP89 cells	969:978	RP89 cells	969:978	In vivo cell apoptosis was evaluated by a TUNEL test and RP89 cells were identified by human mitochondria immunostaining.
25182372	8	5	theme	In	1141:1142	arg1	viability					1155:1163	In vitro cell viability	1141:1163	In vitro cell viability	1141:1163	In vitro cell viability was above 80% after 2 days but decreased significantly after 7 days (60-40%).
25182372	3	6	theme	experimental	668:679	arg1	formulations					690:701	the different experimental hydrogel formulations	654:701	the different experimental hydrogel formulations	654:701	A characterized SCAP cell line (RP89 cells) was encapsulated in the different experimental hydrogel formulations.
25182372	13	7	theme	acid-based	1720:1729	arg1	formulation					1731:1741	a commercially available hyaluronic acid-based formulation	1684:1741	a commercially available hyaluronic acid-based formulation	1684:1741	SIGNIFICANCE Collectively, these data demonstrate that SCAP viability was directly modulated by hydrogel composition and suggest that a commercially available hyaluronic acid-based formulation might be a suitable delivery vehicle for SCAP-based dental pulp regeneration strategies.
25182372	13	7	theme	acid-based	1720:1729	arg1	vehicle					1772:1778	a suitable delivery vehicle	1752:1778	a suitable delivery vehicle for SCAP-based dental pulp regeneration strategies	1752:1829	SIGNIFICANCE Collectively, these data demonstrate that SCAP viability was directly modulated by hydrogel composition and suggest that a commercially available hyaluronic acid-based formulation might be a suitable delivery vehicle for SCAP-based dental pulp regeneration strategies.
25182372	13	8	theme	suitable	1754:1761	arg1	formulation					1731:1741	a commercially available hyaluronic acid-based formulation	1684:1741	a commercially available hyaluronic acid-based formulation	1684:1741	SIGNIFICANCE Collectively, these data demonstrate that SCAP viability was directly modulated by hydrogel composition and suggest that a commercially available hyaluronic acid-based formulation might be a suitable delivery vehicle for SCAP-based dental pulp regeneration strategies.
25182372	13	8	theme	suitable	1754:1761	arg1	vehicle					1772:1778	a suitable delivery vehicle	1752:1778	a suitable delivery vehicle for SCAP-based dental pulp regeneration strategies	1752:1829	SIGNIFICANCE Collectively, these data demonstrate that SCAP viability was directly modulated by hydrogel composition and suggest that a commercially available hyaluronic acid-based formulation might be a suitable delivery vehicle for SCAP-based dental pulp regeneration strategies.
25182372	0	9	theme	stem	95:98	arg1	cells					100:104	stem cells	95:104	stem cells	95:104	The type and composition of alginate and hyaluronic-based hydrogels influence the viability of stem cells of the apical papilla.
25182372	3	10	theme	hydrogel	681:688	arg1	formulations					690:701	the different experimental hydrogel formulations	654:701	the different experimental hydrogel formulations	654:701	A characterized SCAP cell line (RP89 cells) was encapsulated in the different experimental hydrogel formulations.
25182372	4	11	theme	cyclosporine	750:761	arg1	mice					771:774	cyclosporine treated mice	750:774	cyclosporine treated mice	750:774	Cells were cultured in vitro, or implanted in cyclosporine treated mice.
25182372	13	12	theme	delivery	1763:1770	arg1	formulation					1731:1741	a commercially available hyaluronic acid-based formulation	1684:1741	a commercially available hyaluronic acid-based formulation	1684:1741	SIGNIFICANCE Collectively, these data demonstrate that SCAP viability was directly modulated by hydrogel composition and suggest that a commercially available hyaluronic acid-based formulation might be a suitable delivery vehicle for SCAP-based dental pulp regeneration strategies.
25182372	13	12	theme	delivery	1763:1770	arg1	vehicle					1772:1778	a suitable delivery vehicle	1752:1778	a suitable delivery vehicle for SCAP-based dental pulp regeneration strategies	1752:1829	SIGNIFICANCE Collectively, these data demonstrate that SCAP viability was directly modulated by hydrogel composition and suggest that a commercially available hyaluronic acid-based formulation might be a suitable delivery vehicle for SCAP-based dental pulp regeneration strategies.
25182372	1	13	theme	various	222:228	arg1	types					230:234	types	230:234	types	230:234	OBJECTIVE The goal of the present work was to evaluate in vitro and in vivo the influence of various types and compositions of natural hydrogels on the viability and metabolic activity of SCAPs.
25182372	8	14	theme	cell	1150:1153	arg1	viability					1155:1163	In vitro cell viability	1141:1163	In vitro cell viability	1141:1163	In vitro cell viability was above 80% after 2 days but decreased significantly after 7 days (60-40%).
25182372	1	15	theme	SCAPs	317:321	arg1	activity					305:312	metabolic activity	295:312	metabolic activity	295:312	OBJECTIVE The goal of the present work was to evaluate in vitro and in vivo the influence of various types and compositions of natural hydrogels on the viability and metabolic activity of SCAPs.
25182372	1	15	theme	SCAPs	317:321	arg1	viability					281:289	viability	281:289	viability	281:289	OBJECTIVE The goal of the present work was to evaluate in vitro and in vivo the influence of various types and compositions of natural hydrogels on the viability and metabolic activity of SCAPs.
25182372	11	16	theme	low	1454:1456	arg1	%					1471:1471	<1%	1469:1471	<1%	1469:1471	SCAPs survived after 1 week in vivo with low apoptosis (<1%).
25182372	11	16	theme	low	1454:1456	arg1	apoptosis					1458:1466	low apoptosis	1454:1466	low apoptosis (<1%)	1454:1472	SCAPs survived after 1 week in vivo with low apoptosis (<1%).
25182372	1	17	theme	types	230:234	arg1	influence					209:217	the influence	205:217	the influence of various types and compositions of natural hydrogels on the viability and metabolic activity of SCAPs	205:321	OBJECTIVE The goal of the present work was to evaluate in vitro and in vivo the influence of various types and compositions of natural hydrogels on the viability and metabolic activity of SCAPs.
25182372	9	18	from	day	1256:1258	arg1	Viability					1243:1251	Viability	1243:1251	Viability at day 7	1243:1260	Viability at day 7 was the highest in Matrigel (70%) and then in Corgel 1.5 (60%).
25182372	9	18	from	day	1256:1258	arg1	highest					1270:1276	highest	1270:1276	highest	1270:1276	Viability at day 7 was the highest in Matrigel (70%) and then in Corgel 1.5 (60%).
25182372	1	19	dep	viability	281:289	arg1	the					277:279	the	277:279	the	277:279	OBJECTIVE The goal of the present work was to evaluate in vitro and in vivo the influence of various types and compositions of natural hydrogels on the viability and metabolic activity of SCAPs.
25182372	5	20	theme	cellular	852:859	arg1	activity					871:878	in vitro cellular metabolic activity	843:878	in vitro cellular metabolic activity	843:878	In vitro cell viability was evaluated using a Live/Dead assay and in vitro cellular metabolic activity was evaluated with a MTS assay.
25182372	2	21	theme	hyaluronic-based	352:367	arg1	formulations					388:399	three hyaluronic-based (Corgel™) hydrogel formulations	346:399	three hyaluronic-based (Corgel™) hydrogel formulations	346:399	METHODS Two alginate, three hyaluronic-based (Corgel™) hydrogel formulations and Matrigel were characterized for their mechanical, surface and microstructure properties using rheology, X-ray photoelectron spectroscopy and scanning electron microscopy, respectively.
25182372	0	22	theme	cells	100:104	arg1	viability					82:90	the viability	78:90	the viability of stem cells of the apical papilla	78:126	The type and composition of alginate and hyaluronic-based hydrogels influence the viability of stem cells of the apical papilla.
25182372	0	23	theme	alginate	28:35	arg1	hydrogels					58:66	alginate and hyaluronic-based hydrogels	28:66	alginate and hyaluronic-based hydrogels	28:66	The type and composition of alginate and hyaluronic-based hydrogels influence the viability of stem cells of the apical papilla.
25182372	10	24	theme	Metabolic	1326:1334	arg1	activity					1336:1343	Metabolic activity	1326:1343	Metabolic activity	1326:1343	Metabolic activity increased over time in all the hydrogels, excepted in alginate SLM.
25182372	2	25	dep	METHODS	324:330	arg1	Matrigel					405:412	Matrigel	405:412	Matrigel	405:412	METHODS Two alginate, three hyaluronic-based (Corgel™) hydrogel formulations and Matrigel were characterized for their mechanical, surface and microstructure properties using rheology, X-ray photoelectron spectroscopy and scanning electron microscopy, respectively.
25182372	2	25	dep	METHODS	324:330	arg1	alginate					336:343	alginate	336:343	alginate	336:343	METHODS Two alginate, three hyaluronic-based (Corgel™) hydrogel formulations and Matrigel were characterized for their mechanical, surface and microstructure properties using rheology, X-ray photoelectron spectroscopy and scanning electron microscopy, respectively.
25182372	2	25	dep	METHODS	324:330	arg1	METHODS					324:330	METHODS Two	324:334	METHODS Two alginate, three hyaluronic-based (Corgel™) hydrogel formulations and Matrigel	324:412	METHODS Two alginate, three hyaluronic-based (Corgel™) hydrogel formulations and Matrigel were characterized for their mechanical, surface and microstructure properties using rheology, X-ray photoelectron spectroscopy and scanning electron microscopy, respectively.
25182372	2	25	dep	METHODS	324:330	arg1	formulations					388:399	three hyaluronic-based (Corgel™) hydrogel formulations	346:399	three hyaluronic-based (Corgel™) hydrogel formulations	346:399	METHODS Two alginate, three hyaluronic-based (Corgel™) hydrogel formulations and Matrigel were characterized for their mechanical, surface and microstructure properties using rheology, X-ray photoelectron spectroscopy and scanning electron microscopy, respectively.
25182372	1	26	theme	present	155:161	arg1	work					163:166	the present work	151:166	the present work	151:166	OBJECTIVE The goal of the present work was to evaluate in vitro and in vivo the influence of various types and compositions of natural hydrogels on the viability and metabolic activity of SCAPs.
25182372	12	27	theme	highest	1479:1485	arg1	number					1487:1492	The highest number	1475:1492	The highest number of RP89 cells	1475:1506	The highest number of RP89 cells was found in Corgel 5.5 (140cells/mm(2)).
25182372	13	28	dep	Collectively	1563:1574	arg1	SIGNIFICANCE					1550:1561	SIGNIFICANCE	1550:1561	SIGNIFICANCE	1550:1561	SIGNIFICANCE Collectively, these data demonstrate that SCAP viability was directly modulated by hydrogel composition and suggest that a commercially available hyaluronic acid-based formulation might be a suitable delivery vehicle for SCAP-based dental pulp regeneration strategies.
25182372	13	29	theme	hydrogel	1646:1653	arg1	composition					1655:1665	hydrogel composition	1646:1665	hydrogel composition	1646:1665	SIGNIFICANCE Collectively, these data demonstrate that SCAP viability was directly modulated by hydrogel composition and suggest that a commercially available hyaluronic acid-based formulation might be a suitable delivery vehicle for SCAP-based dental pulp regeneration strategies.
25182372	13	30	theme	dental	1795:1800	arg1	strategies					1820:1829	SCAP-based dental pulp regeneration strategies	1784:1829	SCAP-based dental pulp regeneration strategies	1784:1829	SIGNIFICANCE Collectively, these data demonstrate that SCAP viability was directly modulated by hydrogel composition and suggest that a commercially available hyaluronic acid-based formulation might be a suitable delivery vehicle for SCAP-based dental pulp regeneration strategies.
25182372	1	31	theme	work	163:166	arg1	goal					143:146	The goal	139:146	The goal of the present work	139:166	OBJECTIVE The goal of the present work was to evaluate in vitro and in vivo the influence of various types and compositions of natural hydrogels on the viability and metabolic activity of SCAPs.
25182372	2	32	theme	photoelectron	515:527	arg1	spectroscopy					529:540	X-ray photoelectron spectroscopy	509:540	X-ray photoelectron spectroscopy	509:540	METHODS Two alginate, three hyaluronic-based (Corgel™) hydrogel formulations and Matrigel were characterized for their mechanical, surface and microstructure properties using rheology, X-ray photoelectron spectroscopy and scanning electron microscopy, respectively.
25182372	5	33	dep	In	777:778	arg1	vitro					780:784	vitro	780:784	vitro	780:784	In vitro cell viability was evaluated using a Live/Dead assay and in vitro cellular metabolic activity was evaluated with a MTS assay.
25182372	12	34	theme	RP89	1497:1500	arg1	cells					1502:1506	RP89 cells	1497:1506	RP89 cells	1497:1506	The highest number of RP89 cells was found in Corgel 5.5 (140cells/mm(2)).
25182372	5	35	theme	metabolic	861:869	arg1	activity					871:878	in vitro cellular metabolic activity	843:878	in vitro cellular metabolic activity	843:878	In vitro cell viability was evaluated using a Live/Dead assay and in vitro cellular metabolic activity was evaluated with a MTS assay.
25182372	13	36	theme	SCAP	1605:1608	arg1	viability					1610:1618	SCAP viability	1605:1618	SCAP viability	1605:1618	SIGNIFICANCE Collectively, these data demonstrate that SCAP viability was directly modulated by hydrogel composition and suggest that a commercially available hyaluronic acid-based formulation might be a suitable delivery vehicle for SCAP-based dental pulp regeneration strategies.
25182372	1	37	theme	compositions	240:251	arg1	influence					209:217	the influence	205:217	the influence of various types and compositions of natural hydrogels on the viability and metabolic activity of SCAPs	205:321	OBJECTIVE The goal of the present work was to evaluate in vitro and in vivo the influence of various types and compositions of natural hydrogels on the viability and metabolic activity of SCAPs.
25182372	0	38	theme	apical	113:118	arg1	papilla					120:126	the apical papilla	109:126	the apical papilla	109:126	The type and composition of alginate and hyaluronic-based hydrogels influence the viability of stem cells of the apical papilla.
25182372	1	39	dep	OBJECTIVE	129:137	arg1	was					168:170	was	168:170	was to evaluate in vitro and in vivo the influence of various types and compositions of natural hydrogels on the viability and metabolic activity of SCAPs	168:321	OBJECTIVE The goal of the present work was to evaluate in vitro and in vivo the influence of various types and compositions of natural hydrogels on the viability and metabolic activity of SCAPs.
25182372	2	40	theme	hydrogel	379:386	arg1	formulations					388:399	three hyaluronic-based (Corgel™) hydrogel formulations	346:399	three hyaluronic-based (Corgel™) hydrogel formulations	346:399	METHODS Two alginate, three hyaluronic-based (Corgel™) hydrogel formulations and Matrigel were characterized for their mechanical, surface and microstructure properties using rheology, X-ray photoelectron spectroscopy and scanning electron microscopy, respectively.
25182372	13	41	theme	pulp	1802:1805	arg1	strategies					1820:1829	SCAP-based dental pulp regeneration strategies	1784:1829	SCAP-based dental pulp regeneration strategies	1784:1829	SIGNIFICANCE Collectively, these data demonstrate that SCAP viability was directly modulated by hydrogel composition and suggest that a commercially available hyaluronic acid-based formulation might be a suitable delivery vehicle for SCAP-based dental pulp regeneration strategies.
25182372	7	42	theme	RESULTS	1034:1040	arg1	composition					1051:1061	RESULTS Hydrogel composition	1034:1061	RESULTS Hydrogel composition	1034:1061	RESULTS Hydrogel composition influenced their mechanical and surface properties, and their microstructure.
25182372	6	43	dep	In	912:913	arg1	vivo					915:918	vivo	915:918	vivo	915:918	In vivo cell apoptosis was evaluated by a TUNEL test and RP89 cells were identified by human mitochondria immunostaining.
25182372	13	44	theme	available	1699:1707	arg1	formulation					1731:1741	a commercially available hyaluronic acid-based formulation	1684:1741	a commercially available hyaluronic acid-based formulation	1684:1741	SIGNIFICANCE Collectively, these data demonstrate that SCAP viability was directly modulated by hydrogel composition and suggest that a commercially available hyaluronic acid-based formulation might be a suitable delivery vehicle for SCAP-based dental pulp regeneration strategies.
25182372	13	44	theme	available	1699:1707	arg1	vehicle					1772:1778	a suitable delivery vehicle	1752:1778	a suitable delivery vehicle for SCAP-based dental pulp regeneration strategies	1752:1829	SIGNIFICANCE Collectively, these data demonstrate that SCAP viability was directly modulated by hydrogel composition and suggest that a commercially available hyaluronic acid-based formulation might be a suitable delivery vehicle for SCAP-based dental pulp regeneration strategies.
25182372	5	45	theme	Live/Dead	823:831	arg1	assay					833:837	a Live/Dead assay	821:837	a Live/Dead assay	821:837	In vitro cell viability was evaluated using a Live/Dead assay and in vitro cellular metabolic activity was evaluated with a MTS assay.
25182372	2	46	theme	mechanical	443:452	arg1	properties					482:491	their mechanical, surface and microstructure properties	437:491	properties	482:491	METHODS Two alginate, three hyaluronic-based (Corgel™) hydrogel formulations and Matrigel were characterized for their mechanical, surface and microstructure properties using rheology, X-ray photoelectron spectroscopy and scanning electron microscopy, respectively.
25182372	1	47	theme	natural	256:262	arg1	hydrogels					264:272	natural hydrogels	256:272	natural hydrogels	256:272	OBJECTIVE The goal of the present work was to evaluate in vitro and in vivo the influence of various types and compositions of natural hydrogels on the viability and metabolic activity of SCAPs.
25182372	1	48	from	influence	209:217	arg1	activity					305:312	metabolic activity	295:312	metabolic activity	295:312	OBJECTIVE The goal of the present work was to evaluate in vitro and in vivo the influence of various types and compositions of natural hydrogels on the viability and metabolic activity of SCAPs.
25182372	1	48	from	influence	209:217	arg1	viability					281:289	viability	281:289	viability	281:289	OBJECTIVE The goal of the present work was to evaluate in vitro and in vivo the influence of various types and compositions of natural hydrogels on the viability and metabolic activity of SCAPs.
25182372	6	49	theme	mitochondria	1005:1016	arg1	immunostaining					1018:1031	human mitochondria immunostaining	999:1031	human mitochondria immunostaining	999:1031	In vivo cell apoptosis was evaluated by a TUNEL test and RP89 cells were identified by human mitochondria immunostaining.
25182372	8	50	dep	In	1141:1142	arg1	vitro					1144:1148	vitro	1144:1148	vitro	1144:1148	In vitro cell viability was above 80% after 2 days but decreased significantly after 7 days (60-40%).
25182372	12	51	theme	cells	1502:1506	arg1	number					1487:1492	The highest number	1475:1492	The highest number of RP89 cells	1475:1506	The highest number of RP89 cells was found in Corgel 5.5 (140cells/mm(2)).
25182372	2	52	theme	Corgel™	370:376	arg1	formulations					388:399	three hyaluronic-based (Corgel™) hydrogel formulations	346:399	three hyaluronic-based (Corgel™) hydrogel formulations	346:399	METHODS Two alginate, three hyaluronic-based (Corgel™) hydrogel formulations and Matrigel were characterized for their mechanical, surface and microstructure properties using rheology, X-ray photoelectron spectroscopy and scanning electron microscopy, respectively.
25182372	5	53	theme	in	843:844	arg1	activity					871:878	in vitro cellular metabolic activity	843:878	in vitro cellular metabolic activity	843:878	In vitro cell viability was evaluated using a Live/Dead assay and in vitro cellular metabolic activity was evaluated with a MTS assay.
25182372	1	54	theme	hydrogels	264:272	arg1	compositions					240:251	compositions	240:251	compositions	240:251	OBJECTIVE The goal of the present work was to evaluate in vitro and in vivo the influence of various types and compositions of natural hydrogels on the viability and metabolic activity of SCAPs.
25182372	1	54	theme	hydrogels	264:272	arg1	types					230:234	types	230:234	types	230:234	OBJECTIVE The goal of the present work was to evaluate in vitro and in vivo the influence of various types and compositions of natural hydrogels on the viability and metabolic activity of SCAPs.
25182372	0	55	theme	papilla	120:126	arg1	viability					82:90	the viability	78:90	the viability of stem cells of the apical papilla	78:126	The type and composition of alginate and hyaluronic-based hydrogels influence the viability of stem cells of the apical papilla.
25182372	3	56	theme	RP89	622:625	arg1	line					616:619	A characterized SCAP cell line	590:619	A characterized SCAP cell line (RP89 cells)	590:632	A characterized SCAP cell line (RP89 cells) was encapsulated in the different experimental hydrogel formulations.
25182372	3	56	theme	RP89	622:625	arg1	cells					627:631	RP89 cells	622:631	RP89 cells	622:631	A characterized SCAP cell line (RP89 cells) was encapsulated in the different experimental hydrogel formulations.
25182372	3	57	theme	characterized	592:604	arg1	line					616:619	A characterized SCAP cell line	590:619	A characterized SCAP cell line (RP89 cells)	590:632	A characterized SCAP cell line (RP89 cells) was encapsulated in the different experimental hydrogel formulations.
25182372	3	57	theme	characterized	592:604	arg1	cells					627:631	RP89 cells	622:631	RP89 cells	622:631	A characterized SCAP cell line (RP89 cells) was encapsulated in the different experimental hydrogel formulations.
25182372	6	58	theme	human	999:1003	arg1	immunostaining					1018:1031	human mitochondria immunostaining	999:1031	human mitochondria immunostaining	999:1031	In vivo cell apoptosis was evaluated by a TUNEL test and RP89 cells were identified by human mitochondria immunostaining.
25182372	0	59	theme	hyaluronic-based	41:56	arg1	hydrogels					58:66	alginate and hyaluronic-based hydrogels	28:66	alginate and hyaluronic-based hydrogels	28:66	The type and composition of alginate and hyaluronic-based hydrogels influence the viability of stem cells of the apical papilla.
25182372	6	60	theme	cell	920:923	arg1	apoptosis					925:933	In vivo cell apoptosis	912:933	In vivo cell apoptosis	912:933	In vivo cell apoptosis was evaluated by a TUNEL test and RP89 cells were identified by human mitochondria immunostaining.
25182372	5	61	theme	cell	786:789	arg1	viability					791:799	In vitro cell viability	777:799	In vitro cell viability	777:799	In vitro cell viability was evaluated using a Live/Dead assay and in vitro cellular metabolic activity was evaluated with a MTS assay.
25182372	5	62	dep	in	843:844	arg1	vitro					846:850	vitro	846:850	vitro	846:850	In vitro cell viability was evaluated using a Live/Dead assay and in vitro cellular metabolic activity was evaluated with a MTS assay.
25182372	7	63	theme	Hydrogel	1042:1049	arg1	composition					1051:1061	RESULTS Hydrogel composition	1034:1061	RESULTS Hydrogel composition	1034:1061	RESULTS Hydrogel composition influenced their mechanical and surface properties, and their microstructure.
25182372	2	64	theme	X-ray	509:513	arg1	spectroscopy					529:540	X-ray photoelectron spectroscopy	509:540	X-ray photoelectron spectroscopy	509:540	METHODS Two alginate, three hyaluronic-based (Corgel™) hydrogel formulations and Matrigel were characterized for their mechanical, surface and microstructure properties using rheology, X-ray photoelectron spectroscopy and scanning electron microscopy, respectively.
25182372	10	65	theme	alginate	1399:1406	arg1	SLM					1408:1410	alginate SLM	1399:1410	alginate SLM	1399:1410	Metabolic activity increased over time in all the hydrogels, excepted in alginate SLM.
25182372	7	66	theme	surface	1095:1101	arg1	properties					1103:1112	their mechanical and surface properties	1074:1112	properties	1103:1112	RESULTS Hydrogel composition influenced their mechanical and surface properties, and their microstructure.
25182372	5	67	theme	MTS	901:903	arg1	assay					905:909	a MTS assay	899:909	a MTS assay	899:909	In vitro cell viability was evaluated using a Live/Dead assay and in vitro cellular metabolic activity was evaluated with a MTS assay.
25182372	8	68	dep	decreased	1196:1204	arg1	%					1239:1239	60-40%	1234:1239	60-40%	1234:1239	In vitro cell viability was above 80% after 2 days but decreased significantly after 7 days (60-40%).
25182372	6	69	theme	In	912:913	arg1	apoptosis					925:933	In vivo cell apoptosis	912:933	In vivo cell apoptosis	912:933	In vivo cell apoptosis was evaluated by a TUNEL test and RP89 cells were identified by human mitochondria immunostaining.
25182372	2	70	theme	electron	555:562	arg1	microscopy					564:573	scanning electron microscopy	546:573	scanning electron microscopy	546:573	METHODS Two alginate, three hyaluronic-based (Corgel™) hydrogel formulations and Matrigel were characterized for their mechanical, surface and microstructure properties using rheology, X-ray photoelectron spectroscopy and scanning electron microscopy, respectively.
25182372	3	71	theme	different	658:666	arg1	formulations					690:701	the different experimental hydrogel formulations	654:701	the different experimental hydrogel formulations	654:701	A characterized SCAP cell line (RP89 cells) was encapsulated in the different experimental hydrogel formulations.
25182372	5	72	theme	In	777:778	arg1	viability					791:799	In vitro cell viability	777:799	In vitro cell viability	777:799	In vitro cell viability was evaluated using a Live/Dead assay and in vitro cellular metabolic activity was evaluated with a MTS assay.
25182372	3	73	theme	SCAP	606:609	arg1	line					616:619	A characterized SCAP cell line	590:619	A characterized SCAP cell line (RP89 cells)	590:632	A characterized SCAP cell line (RP89 cells) was encapsulated in the different experimental hydrogel formulations.
25182372	3	73	theme	SCAP	606:609	arg1	cells					627:631	RP89 cells	622:631	RP89 cells	622:631	A characterized SCAP cell line (RP89 cells) was encapsulated in the different experimental hydrogel formulations.
25182372	2	74	theme	scanning	546:553	arg1	microscopy					564:573	scanning electron microscopy	546:573	scanning electron microscopy	546:573	METHODS Two alginate, three hyaluronic-based (Corgel™) hydrogel formulations and Matrigel were characterized for their mechanical, surface and microstructure properties using rheology, X-ray photoelectron spectroscopy and scanning electron microscopy, respectively.
25182372	13	75	theme	hyaluronic	1709:1718	arg1	formulation					1731:1741	a commercially available hyaluronic acid-based formulation	1684:1741	a commercially available hyaluronic acid-based formulation	1684:1741	SIGNIFICANCE Collectively, these data demonstrate that SCAP viability was directly modulated by hydrogel composition and suggest that a commercially available hyaluronic acid-based formulation might be a suitable delivery vehicle for SCAP-based dental pulp regeneration strategies.
25182372	13	75	theme	hyaluronic	1709:1718	arg1	vehicle					1772:1778	a suitable delivery vehicle	1752:1778	a suitable delivery vehicle for SCAP-based dental pulp regeneration strategies	1752:1829	SIGNIFICANCE Collectively, these data demonstrate that SCAP viability was directly modulated by hydrogel composition and suggest that a commercially available hyaluronic acid-based formulation might be a suitable delivery vehicle for SCAP-based dental pulp regeneration strategies.
25182372	13	76	theme	SCAP-based	1784:1793	arg1	strategies					1820:1829	SCAP-based dental pulp regeneration strategies	1784:1829	SCAP-based dental pulp regeneration strategies	1784:1829	SIGNIFICANCE Collectively, these data demonstrate that SCAP viability was directly modulated by hydrogel composition and suggest that a commercially available hyaluronic acid-based formulation might be a suitable delivery vehicle for SCAP-based dental pulp regeneration strategies.
25182372	2	77	theme	microstructure	467:480	arg1	properties					482:491	their mechanical, surface and microstructure properties	437:491	properties	482:491	METHODS Two alginate, three hyaluronic-based (Corgel™) hydrogel formulations and Matrigel were characterized for their mechanical, surface and microstructure properties using rheology, X-ray photoelectron spectroscopy and scanning electron microscopy, respectively.
25182372	0	78	dep	type	4:7	arg1	The					0:2	The	0:2	The	0:2	The type and composition of alginate and hyaluronic-based hydrogels influence the viability of stem cells of the apical papilla.
25182372	13	79	theme	regeneration	1807:1818	arg1	strategies					1820:1829	SCAP-based dental pulp regeneration strategies	1784:1829	SCAP-based dental pulp regeneration strategies	1784:1829	SIGNIFICANCE Collectively, these data demonstrate that SCAP viability was directly modulated by hydrogel composition and suggest that a commercially available hyaluronic acid-based formulation might be a suitable delivery vehicle for SCAP-based dental pulp regeneration strategies.
25182372	0	80	theme	hydrogels	58:66	arg1	composition					13:23	composition	13:23	composition	13:23	The type and composition of alginate and hyaluronic-based hydrogels influence the viability of stem cells of the apical papilla.
25182372	0	80	theme	hydrogels	58:66	arg1	type					4:7	type	4:7	type	4:7	The type and composition of alginate and hyaluronic-based hydrogels influence the viability of stem cells of the apical papilla.
25182372	12	81	located	found	1512:1516	arg2	number					1487:1492	The highest number	1475:1492	The highest number of RP89 cells	1475:1506	The highest number of RP89 cells was found in Corgel 5.5 (140cells/mm(2)).
25182372	12	81	located	found	1512:1516	arg1	Corgel					1521:1526	Corgel	1521:1526	Corgel	1521:1526	The highest number of RP89 cells was found in Corgel 5.5 (140cells/mm(2)).
25182372	12	81	located	found	1512:1516	arg1	140cells/mm					1533:1543	140cells/mm(2)	1533:1546	140cells/mm(2)	1533:1546	The highest number of RP89 cells was found in Corgel 5.5 (140cells/mm(2)).
25182372	3	82	theme	cell	611:614	arg1	line					616:619	A characterized SCAP cell line	590:619	A characterized SCAP cell line (RP89 cells)	590:632	A characterized SCAP cell line (RP89 cells) was encapsulated in the different experimental hydrogel formulations.
25182372	3	82	theme	cell	611:614	arg1	cells					627:631	RP89 cells	622:631	RP89 cells	622:631	A characterized SCAP cell line (RP89 cells) was encapsulated in the different experimental hydrogel formulations.
28671532	11	0	theme	S66T	1297:1300	arg1	sequence					1285:1292	the genome sequence	1274:1292	the genome sequence of S66T	1274:1300	Based on the genome sequence of S66T, the average nucleotide identity (ANI) between strain S66T and other members of the genus Paraglaciecola was 77-80 %, and DNA-DNA hybridization prediction showed values of less than 24 % relatedness, respectively, between S66T and other species of the genus Paraglaciecola.
28671532	2	1	theme	bacterial	132:140	arg1	S66T					150:153	S66T	150:153	S66T	150:153	A novel bacterial strain, S66T, was isolated from eelgrass collected on the coastline of Zealand, Denmark.
28671532	2	1	theme	bacterial	132:140	arg1	strain					142:147	A novel bacterial strain	124:147	A novel bacterial strain	124:147	A novel bacterial strain, S66T, was isolated from eelgrass collected on the coastline of Zealand, Denmark.
28671532	5	2	theme	sole	613:616	arg1	sources					625:631	sole carbon sources	613:631	sole carbon sources	613:631	Furthermore, strain S66T grew on seaweed polysaccharides agar, agarose, porphyran, κ-carrageenan, alginate and laminarin as sole carbon sources.
28671532	11	3	theme	genus	1554:1558	arg1	Paraglaciecola					1560:1573	the genus Paraglaciecola	1550:1573	the genus Paraglaciecola	1550:1573	Based on the genome sequence of S66T, the average nucleotide identity (ANI) between strain S66T and other members of the genus Paraglaciecola was 77-80 %, and DNA-DNA hybridization prediction showed values of less than 24 % relatedness, respectively, between S66T and other species of the genus Paraglaciecola.
28671532	9	4	theme	Gammaproteobacteria	1072:1090	arg1	Alteromonadaceae					1042:1057	the family Alteromonadaceae	1031:1057	the family Alteromonadaceae of the class Gammaproteobacteria	1031:1090	Phylogenetic analyses based on the 16S rRNA gene and GyrB sequence comparisons showed that the bacterium was affiliated with the genus Paraglaciecola within the family Alteromonadaceae of the class Gammaproteobacteria.
28671532	1	5	link	seaweed-derived	91:105	arg1	polysaccharides					107:121	multiple seaweed-derived polysaccharides	82:121	multiple seaweed-derived polysaccharides	82:121	nov., a bacterium with hydrolytic activity against multiple seaweed-derived polysaccharides.
28671532	2	6	theme	novel	126:130	arg1	S66T					150:153	S66T	150:153	S66T	150:153	A novel bacterial strain, S66T, was isolated from eelgrass collected on the coastline of Zealand, Denmark.
28671532	2	6	theme	novel	126:130	arg1	strain					142:147	A novel bacterial strain	124:147	A novel bacterial strain	124:147	A novel bacterial strain, S66T, was isolated from eelgrass collected on the coastline of Zealand, Denmark.
28671532	5	7	theme	carbon	618:623	arg1	sources					625:631	sole carbon sources	613:631	sole carbon sources	613:631	Furthermore, strain S66T grew on seaweed polysaccharides agar, agarose, porphyran, κ-carrageenan, alginate and laminarin as sole carbon sources.
28671532	11	8	theme	genus	1386:1390	arg1	Paraglaciecola					1392:1405	the genus Paraglaciecola	1382:1405	the genus Paraglaciecola	1382:1405	Based on the genome sequence of S66T, the average nucleotide identity (ANI) between strain S66T and other members of the genus Paraglaciecola was 77-80 %, and DNA-DNA hybridization prediction showed values of less than 24 % relatedness, respectively, between S66T and other species of the genus Paraglaciecola.
28671532	12	9	theme	phylogenetic	1592:1603	arg1	analyses					1617:1624	The phenotypic, phylogenetic and genomic analyses	1576:1624	The phenotypic, phylogenetic and genomic analyses	1576:1624	The phenotypic, phylogenetic and genomic analyses support the hypothesis that strain S66T represents a novel species of the genus Paraglaciecola, for which the name Paraglaciecola hydrolytica sp.
28671532	11	10	theme	other	1533:1537	arg1	species					1539:1545	other species	1533:1545	other species of the genus Paraglaciecola	1533:1573	Based on the genome sequence of S66T, the average nucleotide identity (ANI) between strain S66T and other members of the genus Paraglaciecola was 77-80 %, and DNA-DNA hybridization prediction showed values of less than 24 % relatedness, respectively, between S66T and other species of the genus Paraglaciecola.
28671532	12	11	theme	novel	1679:1683	arg1	species					1685:1691	a novel species	1677:1691	a novel species	1677:1691	The phenotypic, phylogenetic and genomic analyses support the hypothesis that strain S66T represents a novel species of the genus Paraglaciecola, for which the name Paraglaciecola hydrolytica sp.
28671532	10	12	theme	members	1189:1195	arg1	gene					1140:1143	the 16S rRNA gene and GyrB sequences	1127:1162	gene	1140:1143	The percentage similarity between the 16S rRNA gene and GyrB sequences of strain S66T and other members of the genus Paraglaciecola were 94-95 % and 84-85 %, respectively.
28671532	10	12	theme	members	1189:1195	arg1	sequences					1154:1162	the 16S rRNA gene and GyrB sequences	1127:1162	sequences	1154:1162	The percentage similarity between the 16S rRNA gene and GyrB sequences of strain S66T and other members of the genus Paraglaciecola were 94-95 % and 84-85 %, respectively.
28671532	10	13	theme	GyrB	1149:1152	arg1	sequences					1154:1162	the 16S rRNA gene and GyrB sequences	1127:1162	sequences	1154:1162	The percentage similarity between the 16S rRNA gene and GyrB sequences of strain S66T and other members of the genus Paraglaciecola were 94-95 % and 84-85 %, respectively.
28671532	9	14	theme	Phylogenetic	874:885	arg1	analyses					887:894	Phylogenetic analyses	874:894	Phylogenetic analyses based on the 16S rRNA gene	874:921	Phylogenetic analyses based on the 16S rRNA gene and GyrB sequence comparisons showed that the bacterium was affiliated with the genus Paraglaciecola within the family Alteromonadaceae of the class Gammaproteobacteria.
28671532	11	15	theme	Paraglaciecola	1560:1573	arg1	species					1539:1545	other species	1533:1545	other species of the genus Paraglaciecola	1533:1573	Based on the genome sequence of S66T, the average nucleotide identity (ANI) between strain S66T and other members of the genus Paraglaciecola was 77-80 %, and DNA-DNA hybridization prediction showed values of less than 24 % relatedness, respectively, between S66T and other species of the genus Paraglaciecola.
28671532	9	16	theme	family	1035:1040	arg1	Alteromonadaceae					1042:1057	the family Alteromonadaceae	1031:1057	the family Alteromonadaceae of the class Gammaproteobacteria	1031:1090	Phylogenetic analyses based on the 16S rRNA gene and GyrB sequence comparisons showed that the bacterium was affiliated with the genus Paraglaciecola within the family Alteromonadaceae of the class Gammaproteobacteria.
28671532	11	17	theme	DNA-DNA	1424:1430	arg1	prediction					1446:1455	DNA-DNA hybridization prediction	1424:1455	DNA-DNA hybridization prediction	1424:1455	Based on the genome sequence of S66T, the average nucleotide identity (ANI) between strain S66T and other members of the genus Paraglaciecola was 77-80 %, and DNA-DNA hybridization prediction showed values of less than 24 % relatedness, respectively, between S66T and other species of the genus Paraglaciecola.
28671532	1	18	theme	hydrolytic	54:63	arg1	activity					65:72	hydrolytic activity	54:72	hydrolytic activity against multiple seaweed-derived polysaccharides	54:121	nov., a bacterium with hydrolytic activity against multiple seaweed-derived polysaccharides.
28671532	10	19	theme	%	1236:1236	arg1	similarity					1108:1117	The percentage similarity	1093:1117	The percentage similarity between the 16S rRNA gene and GyrB sequences of strain S66T and other members of the genus Paraglaciecola	1093:1223	The percentage similarity between the 16S rRNA gene and GyrB sequences of strain S66T and other members of the genus Paraglaciecola were 94-95 % and 84-85 %, respectively.
28671532	10	19	theme	%	1236:1236	arg1	%					1248:1248	94-95 % and 84-85 %	1230:1248	94-95 % and 84-85 %	1230:1248	The percentage similarity between the 16S rRNA gene and GyrB sequences of strain S66T and other members of the genus Paraglaciecola were 94-95 % and 84-85 %, respectively.
28671532	11	20	theme	nucleotide	1315:1324	arg1	identity					1326:1333	the average nucleotide identity	1303:1333	the average nucleotide identity (ANI) between strain S66T and other members of the genus Paraglaciecola	1303:1405	Based on the genome sequence of S66T, the average nucleotide identity (ANI) between strain S66T and other members of the genus Paraglaciecola was 77-80 %, and DNA-DNA hybridization prediction showed values of less than 24 % relatedness, respectively, between S66T and other species of the genus Paraglaciecola.
28671532	11	20	theme	nucleotide	1315:1324	arg1	%					1417:1417	77-80 %	1411:1417	77-80 %	1411:1417	Based on the genome sequence of S66T, the average nucleotide identity (ANI) between strain S66T and other members of the genus Paraglaciecola was 77-80 %, and DNA-DNA hybridization prediction showed values of less than 24 % relatedness, respectively, between S66T and other species of the genus Paraglaciecola.
28671532	11	20	theme	nucleotide	1315:1324	arg1	ANI					1336:1338	ANI	1336:1338	ANI	1336:1338	Based on the genome sequence of S66T, the average nucleotide identity (ANI) between strain S66T and other members of the genus Paraglaciecola was 77-80 %, and DNA-DNA hybridization prediction showed values of less than 24 % relatedness, respectively, between S66T and other species of the genus Paraglaciecola.
28671532	11	21	theme	strain	1349:1354	arg1	S66T					1356:1359	strain S66T	1349:1359	strain S66T	1349:1359	Based on the genome sequence of S66T, the average nucleotide identity (ANI) between strain S66T and other members of the genus Paraglaciecola was 77-80 %, and DNA-DNA hybridization prediction showed values of less than 24 % relatedness, respectively, between S66T and other species of the genus Paraglaciecola.
28671532	11	22	theme	hybridization	1432:1444	arg1	prediction					1446:1455	DNA-DNA hybridization prediction	1424:1455	DNA-DNA hybridization prediction	1424:1455	Based on the genome sequence of S66T, the average nucleotide identity (ANI) between strain S66T and other members of the genus Paraglaciecola was 77-80 %, and DNA-DNA hybridization prediction showed values of less than 24 % relatedness, respectively, between S66T and other species of the genus Paraglaciecola.
28671532	5	23	dep	polysaccharides	530:544	arg1	agarose					552:558	agarose	552:558	agarose	552:558	Furthermore, strain S66T grew on seaweed polysaccharides agar, agarose, porphyran, κ-carrageenan, alginate and laminarin as sole carbon sources.
28671532	5	23	dep	polysaccharides	530:544	arg1	alginate					587:594	alginate	587:594	alginate	587:594	Furthermore, strain S66T grew on seaweed polysaccharides agar, agarose, porphyran, κ-carrageenan, alginate and laminarin as sole carbon sources.
28671532	5	23	dep	polysaccharides	530:544	arg1	laminarin					600:608	laminarin	600:608	laminarin	600:608	Furthermore, strain S66T grew on seaweed polysaccharides agar, agarose, porphyran, κ-carrageenan, alginate and laminarin as sole carbon sources.
28671532	5	23	dep	polysaccharides	530:544	arg1	porphyran					561:569	porphyran	561:569	porphyran	561:569	Furthermore, strain S66T grew on seaweed polysaccharides agar, agarose, porphyran, κ-carrageenan, alginate and laminarin as sole carbon sources.
28671532	5	23	dep	polysaccharides	530:544	arg1	polysaccharides					530:544	seaweed polysaccharides agar, agarose, porphyran, κ-carrageenan, alginate and laminarin	522:608	seaweed polysaccharides agar, agarose, porphyran, κ-carrageenan, alginate and laminarin	522:608	Furthermore, strain S66T grew on seaweed polysaccharides agar, agarose, porphyran, κ-carrageenan, alginate and laminarin as sole carbon sources.
28671532	5	23	dep	polysaccharides	530:544	arg1	κ-carrageenan					572:584	κ-carrageenan	572:584	κ-carrageenan	572:584	Furthermore, strain S66T grew on seaweed polysaccharides agar, agarose, porphyran, κ-carrageenan, alginate and laminarin as sole carbon sources.
28671532	5	23	dep	polysaccharides	530:544	arg1	agar					546:549	agar	546:549	agar	546:549	Furthermore, strain S66T grew on seaweed polysaccharides agar, agarose, porphyran, κ-carrageenan, alginate and laminarin as sole carbon sources.
28671532	11	24	theme	genome	1278:1283	arg1	sequence					1285:1292	the genome sequence	1274:1292	the genome sequence of S66T	1274:1300	Based on the genome sequence of S66T, the average nucleotide identity (ANI) between strain S66T and other members of the genus Paraglaciecola was 77-80 %, and DNA-DNA hybridization prediction showed values of less than 24 % relatedness, respectively, between S66T and other species of the genus Paraglaciecola.
28671532	9	25	theme	sequence	932:939	arg1	comparisons					941:951	GyrB sequence comparisons	927:951	GyrB sequence comparisons	927:951	Phylogenetic analyses based on the 16S rRNA gene and GyrB sequence comparisons showed that the bacterium was affiliated with the genus Paraglaciecola within the family Alteromonadaceae of the class Gammaproteobacteria.
28671532	4	26	theme	optimum	472:478	arg1	pH					464:465	pH 7-9	464:469	pH 7-9 (optimum pH 7.5)	464:486	The strain was Gram-reaction-negative, rod-shaped, aerobic, and displayed growth at 10-25 °C (optimum 20-25 °C) and at pH 7-9 (optimum pH 7.5).
28671532	4	26	theme	optimum	472:478	arg1	pH					480:481	optimum pH 7.5	472:485	optimum pH 7.5	472:485	The strain was Gram-reaction-negative, rod-shaped, aerobic, and displayed growth at 10-25 °C (optimum 20-25 °C) and at pH 7-9 (optimum pH 7.5).
28671532	3	27	theme	phenotypic	261:270	arg1	methods					298:304	phenotypic, phylogenetic and genomic methods	261:304	phenotypic, phylogenetic and genomic methods	261:304	Polyphasic analyses involving phenotypic, phylogenetic and genomic methods were used to characterize strain S66T.
28671532	8	28	theme	DNA	826:828	arg1	content					834:840	The DNA G+C content	822:840	The DNA G+C content	822:840	The DNA G+C content was determined to be 42.2 mol%.
28671532	8	28	theme	DNA	826:828	arg1	%					871:871	42.2 mol%	863:871	42.2 mol%	863:871	The DNA G+C content was determined to be 42.2 mol%.
28671532	0	29	theme	hydrolytica	15:25	arg1	sp					27:28	Paraglaciecola hydrolytica sp	0:28	Paraglaciecola hydrolytica sp.	0:29	Paraglaciecola hydrolytica sp.
28671532	3	30	theme	strain	332:337	arg1	S66T					339:342	strain S66T	332:342	strain S66T	332:342	Polyphasic analyses involving phenotypic, phylogenetic and genomic methods were used to characterize strain S66T.
28671532	3	31	theme	Polyphasic	231:240	arg1	analyses					242:249	Polyphasic analyses	231:249	Polyphasic analyses involving phenotypic, phylogenetic and genomic methods	231:304	Polyphasic analyses involving phenotypic, phylogenetic and genomic methods were used to characterize strain S66T.
28671532	6	32	dep	C16 	657:660	arg1	 1ω7c					671:675	 1ω7c	671:675	 1ω7c	671:675	Major fatty acids were C16 : 0, C16 : 1ω7c and C18 : 1ω7c.
28671532	6	32	dep	C16 	657:660	arg1	C16 					666:669	C16 	666:669	C16 	666:669	Major fatty acids were C16 : 0, C16 : 1ω7c and C18 : 1ω7c.
28671532	6	32	dep	C16 	657:660	arg1	 1ω7c					686:690	 1ω7c	686:690	 1ω7c	686:690	Major fatty acids were C16 : 0, C16 : 1ω7c and C18 : 1ω7c.
28671532	6	32	dep	C16 	657:660	arg1	C18 					681:684	C18 	681:684	C18 	681:684	Major fatty acids were C16 : 0, C16 : 1ω7c and C18 : 1ω7c.
28671532	1	33	theme	multiple	82:89	arg1	polysaccharides					107:121	multiple seaweed-derived polysaccharides	82:121	multiple seaweed-derived polysaccharides	82:121	nov., a bacterium with hydrolytic activity against multiple seaweed-derived polysaccharides.
28671532	10	34	theme	Paraglaciecola	1210:1223	arg1	S66T					1174:1177	strain S66T	1167:1177	strain S66T	1167:1177	The percentage similarity between the 16S rRNA gene and GyrB sequences of strain S66T and other members of the genus Paraglaciecola were 94-95 % and 84-85 %, respectively.
28671532	10	34	theme	Paraglaciecola	1210:1223	arg1	members					1189:1195	other members	1183:1195	other members of the genus Paraglaciecola	1183:1223	The percentage similarity between the 16S rRNA gene and GyrB sequences of strain S66T and other members of the genus Paraglaciecola were 94-95 % and 84-85 %, respectively.
28671532	0	35	theme	Paraglaciecola	0:13	arg1	sp					27:28	Paraglaciecola hydrolytica sp	0:28	Paraglaciecola hydrolytica sp.	0:29	Paraglaciecola hydrolytica sp.
28671532	12	36	theme	strain	1654:1659	arg1	S66T					1661:1664	strain S66T	1654:1664	strain S66T	1654:1664	The phenotypic, phylogenetic and genomic analyses support the hypothesis that strain S66T represents a novel species of the genus Paraglaciecola, for which the name Paraglaciecola hydrolytica sp.
28671532	1	37	theme	seaweed-derived	91:105	arg1	polysaccharides					107:121	multiple seaweed-derived polysaccharides	82:121	multiple seaweed-derived polysaccharides	82:121	nov., a bacterium with hydrolytic activity against multiple seaweed-derived polysaccharides.
28671532	7	38	theme	major	747:751	arg1	lipids					759:764	major polar lipids	747:764	major polar lipids	747:764	The respiratory quinone was determined to be Q-8, and major polar lipids were phosphatidylethanolamine and phosphatidylglycerol.
28671532	9	39	theme	16S	909:911	arg1	rRNA					913:916	16S rRNA	909:916	the 16S rRNA gene	905:921	Phylogenetic analyses based on the 16S rRNA gene and GyrB sequence comparisons showed that the bacterium was affiliated with the genus Paraglaciecola within the family Alteromonadaceae of the class Gammaproteobacteria.
28671532	10	40	theme	94-95 	1230:1235	arg1	similarity					1108:1117	The percentage similarity	1093:1117	The percentage similarity between the 16S rRNA gene and GyrB sequences of strain S66T and other members of the genus Paraglaciecola	1093:1223	The percentage similarity between the 16S rRNA gene and GyrB sequences of strain S66T and other members of the genus Paraglaciecola were 94-95 % and 84-85 %, respectively.
28671532	10	40	theme	94-95 	1230:1235	arg1	%					1248:1248	94-95 % and 84-85 %	1230:1248	94-95 % and 84-85 %	1230:1248	The percentage similarity between the 16S rRNA gene and GyrB sequences of strain S66T and other members of the genus Paraglaciecola were 94-95 % and 84-85 %, respectively.
28671532	7	41	theme	polar	753:757	arg1	lipids					759:764	major polar lipids	747:764	major polar lipids	747:764	The respiratory quinone was determined to be Q-8, and major polar lipids were phosphatidylethanolamine and phosphatidylglycerol.
28671532	14	42	theme	type	1794:1797	arg1	S66T					1809:1812	S66T	1809:1812	S66T (=LMG 29457T=NCIMB 15060T=DSM 102834T)	1809:1851	The type strain is S66T (=LMG 29457T=NCIMB 15060T=DSM 102834T).
28671532	14	42	theme	type	1794:1797	arg1	strain					1799:1804	The type strain	1790:1804	The type strain	1790:1804	The type strain is S66T (=LMG 29457T=NCIMB 15060T=DSM 102834T).
28671532	9	43	theme	rRNA	913:916	arg1	gene					918:921	the 16S rRNA gene	905:921	the 16S rRNA gene	905:921	Phylogenetic analyses based on the 16S rRNA gene and GyrB sequence comparisons showed that the bacterium was affiliated with the genus Paraglaciecola within the family Alteromonadaceae of the class Gammaproteobacteria.
28671532	3	44	theme	genomic	290:296	arg1	methods					298:304	phenotypic, phylogenetic and genomic methods	261:304	phenotypic, phylogenetic and genomic methods	261:304	Polyphasic analyses involving phenotypic, phylogenetic and genomic methods were used to characterize strain S66T.
28671532	10	45	theme	 and	1237:1240	arg1	similarity					1108:1117	The percentage similarity	1093:1117	The percentage similarity between the 16S rRNA gene and GyrB sequences of strain S66T and other members of the genus Paraglaciecola	1093:1223	The percentage similarity between the 16S rRNA gene and GyrB sequences of strain S66T and other members of the genus Paraglaciecola were 94-95 % and 84-85 %, respectively.
28671532	10	45	theme	 and	1237:1240	arg1	%					1248:1248	94-95 % and 84-85 %	1230:1248	94-95 % and 84-85 %	1230:1248	The percentage similarity between the 16S rRNA gene and GyrB sequences of strain S66T and other members of the genus Paraglaciecola were 94-95 % and 84-85 %, respectively.
28671532	6	46	theme	fatty	640:644	arg1	acids					646:650	Major fatty acids	634:650	Major fatty acids	634:650	Major fatty acids were C16 : 0, C16 : 1ω7c and C18 : 1ω7c.
28671532	11	47	dep	%	1487:1487	arg1	24 					1484:1486	24 	1484:1486	24 	1484:1486	Based on the genome sequence of S66T, the average nucleotide identity (ANI) between strain S66T and other members of the genus Paraglaciecola was 77-80 %, and DNA-DNA hybridization prediction showed values of less than 24 % relatedness, respectively, between S66T and other species of the genus Paraglaciecola.
28671532	11	48	theme	other	1365:1369	arg1	members					1371:1377	other members	1365:1377	other members of the genus Paraglaciecola	1365:1405	Based on the genome sequence of S66T, the average nucleotide identity (ANI) between strain S66T and other members of the genus Paraglaciecola was 77-80 %, and DNA-DNA hybridization prediction showed values of less than 24 % relatedness, respectively, between S66T and other species of the genus Paraglaciecola.
28671532	9	49	theme	genus	1003:1007	arg1	Paraglaciecola					1009:1022	the genus Paraglaciecola	999:1022	the genus Paraglaciecola within the family Alteromonadaceae of the class Gammaproteobacteria	999:1090	Phylogenetic analyses based on the 16S rRNA gene and GyrB sequence comparisons showed that the bacterium was affiliated with the genus Paraglaciecola within the family Alteromonadaceae of the class Gammaproteobacteria.
28671532	6	50	theme	Major	634:638	arg1	acids					646:650	Major fatty acids	634:650	Major fatty acids	634:650	Major fatty acids were C16 : 0, C16 : 1ω7c and C18 : 1ω7c.
28671532	2	51	attach	isolated	160:167	arg1	eelgrass					174:181	eelgrass	174:181	eelgrass collected on the coastline of Zealand, Denmark	174:228	A novel bacterial strain, S66T, was isolated from eelgrass collected on the coastline of Zealand, Denmark.
28671532	2	51	attach	isolated	160:167	arg2	S66T					150:153	S66T	150:153	S66T	150:153	A novel bacterial strain, S66T, was isolated from eelgrass collected on the coastline of Zealand, Denmark.
28671532	2	51	attach	isolated	160:167	arg2	strain					142:147	A novel bacterial strain	124:147	A novel bacterial strain	124:147	A novel bacterial strain, S66T, was isolated from eelgrass collected on the coastline of Zealand, Denmark.
28671532	11	52	theme	Paraglaciecola	1392:1405	arg1	S66T					1356:1359	strain S66T	1349:1359	strain S66T	1349:1359	Based on the genome sequence of S66T, the average nucleotide identity (ANI) between strain S66T and other members of the genus Paraglaciecola was 77-80 %, and DNA-DNA hybridization prediction showed values of less than 24 % relatedness, respectively, between S66T and other species of the genus Paraglaciecola.
28671532	11	52	theme	Paraglaciecola	1392:1405	arg1	members					1371:1377	other members	1365:1377	other members of the genus Paraglaciecola	1365:1405	Based on the genome sequence of S66T, the average nucleotide identity (ANI) between strain S66T and other members of the genus Paraglaciecola was 77-80 %, and DNA-DNA hybridization prediction showed values of less than 24 % relatedness, respectively, between S66T and other species of the genus Paraglaciecola.
28671532	3	53	theme	phylogenetic	273:284	arg1	methods					298:304	phenotypic, phylogenetic and genomic methods	261:304	phenotypic, phylogenetic and genomic methods	261:304	Polyphasic analyses involving phenotypic, phylogenetic and genomic methods were used to characterize strain S66T.
28671532	2	54	theme	Denmark	222:228	arg1	coastline					200:208	the coastline	196:208	the coastline of Zealand, Denmark	196:228	A novel bacterial strain, S66T, was isolated from eelgrass collected on the coastline of Zealand, Denmark.
28671532	11	55	theme	relatedness	1489:1499	arg1	values					1464:1469	values	1464:1469	values of less than 24 % relatedness	1464:1499	Based on the genome sequence of S66T, the average nucleotide identity (ANI) between strain S66T and other members of the genus Paraglaciecola was 77-80 %, and DNA-DNA hybridization prediction showed values of less than 24 % relatedness, respectively, between S66T and other species of the genus Paraglaciecola.
28671532	9	56	theme	GyrB	927:930	arg1	comparisons					941:951	GyrB sequence comparisons	927:951	GyrB sequence comparisons	927:951	Phylogenetic analyses based on the 16S rRNA gene and GyrB sequence comparisons showed that the bacterium was affiliated with the genus Paraglaciecola within the family Alteromonadaceae of the class Gammaproteobacteria.
28671532	12	57	theme	Paraglaciecola	1706:1719	arg1	species					1685:1691	a novel species	1677:1691	a novel species	1677:1691	The phenotypic, phylogenetic and genomic analyses support the hypothesis that strain S66T represents a novel species of the genus Paraglaciecola, for which the name Paraglaciecola hydrolytica sp.
28671532	12	58	theme	name	1736:1739	arg1	sp					1768:1769	the name Paraglaciecola hydrolytica sp	1732:1769	the name Paraglaciecola hydrolytica sp	1732:1769	The phenotypic, phylogenetic and genomic analyses support the hypothesis that strain S66T represents a novel species of the genus Paraglaciecola, for which the name Paraglaciecola hydrolytica sp.
28671532	11	59	theme	average	1307:1313	arg1	identity					1326:1333	the average nucleotide identity	1303:1333	the average nucleotide identity (ANI) between strain S66T and other members of the genus Paraglaciecola	1303:1405	Based on the genome sequence of S66T, the average nucleotide identity (ANI) between strain S66T and other members of the genus Paraglaciecola was 77-80 %, and DNA-DNA hybridization prediction showed values of less than 24 % relatedness, respectively, between S66T and other species of the genus Paraglaciecola.
28671532	11	59	theme	average	1307:1313	arg1	%					1417:1417	77-80 %	1411:1417	77-80 %	1411:1417	Based on the genome sequence of S66T, the average nucleotide identity (ANI) between strain S66T and other members of the genus Paraglaciecola was 77-80 %, and DNA-DNA hybridization prediction showed values of less than 24 % relatedness, respectively, between S66T and other species of the genus Paraglaciecola.
28671532	11	59	theme	average	1307:1313	arg1	ANI					1336:1338	ANI	1336:1338	ANI	1336:1338	Based on the genome sequence of S66T, the average nucleotide identity (ANI) between strain S66T and other members of the genus Paraglaciecola was 77-80 %, and DNA-DNA hybridization prediction showed values of less than 24 % relatedness, respectively, between S66T and other species of the genus Paraglaciecola.
28671532	9	60	theme	class	1066:1070	arg1	Gammaproteobacteria					1072:1090	the class Gammaproteobacteria	1062:1090	the class Gammaproteobacteria	1062:1090	Phylogenetic analyses based on the 16S rRNA gene and GyrB sequence comparisons showed that the bacterium was affiliated with the genus Paraglaciecola within the family Alteromonadaceae of the class Gammaproteobacteria.
28671532	3	61	used	used	311:314	arg2	analyses					242:249	Polyphasic analyses	231:249	Polyphasic analyses involving phenotypic, phylogenetic and genomic methods	231:304	Polyphasic analyses involving phenotypic, phylogenetic and genomic methods were used to characterize strain S66T.
28671532	10	62	theme	percentage	1097:1106	arg1	%					1248:1248	94-95 % and 84-85 %	1230:1248	94-95 % and 84-85 %	1230:1248	The percentage similarity between the 16S rRNA gene and GyrB sequences of strain S66T and other members of the genus Paraglaciecola were 94-95 % and 84-85 %, respectively.
28671532	10	62	theme	percentage	1097:1106	arg1	similarity					1108:1117	The percentage similarity	1093:1117	The percentage similarity between the 16S rRNA gene and GyrB sequences of strain S66T and other members of the genus Paraglaciecola	1093:1223	The percentage similarity between the 16S rRNA gene and GyrB sequences of strain S66T and other members of the genus Paraglaciecola were 94-95 % and 84-85 %, respectively.
28671532	11	63	dep	relatedness	1489:1499	arg1	%					1487:1487	%	1487:1487	%	1487:1487	Based on the genome sequence of S66T, the average nucleotide identity (ANI) between strain S66T and other members of the genus Paraglaciecola was 77-80 %, and DNA-DNA hybridization prediction showed values of less than 24 % relatedness, respectively, between S66T and other species of the genus Paraglaciecola.
28671532	5	64	theme	seaweed	522:528	arg1	agarose					552:558	agarose	552:558	agarose	552:558	Furthermore, strain S66T grew on seaweed polysaccharides agar, agarose, porphyran, κ-carrageenan, alginate and laminarin as sole carbon sources.
28671532	5	64	theme	seaweed	522:528	arg1	alginate					587:594	alginate	587:594	alginate	587:594	Furthermore, strain S66T grew on seaweed polysaccharides agar, agarose, porphyran, κ-carrageenan, alginate and laminarin as sole carbon sources.
28671532	5	64	theme	seaweed	522:528	arg1	laminarin					600:608	laminarin	600:608	laminarin	600:608	Furthermore, strain S66T grew on seaweed polysaccharides agar, agarose, porphyran, κ-carrageenan, alginate and laminarin as sole carbon sources.
28671532	5	64	theme	seaweed	522:528	arg1	porphyran					561:569	porphyran	561:569	porphyran	561:569	Furthermore, strain S66T grew on seaweed polysaccharides agar, agarose, porphyran, κ-carrageenan, alginate and laminarin as sole carbon sources.
28671532	5	64	theme	seaweed	522:528	arg1	polysaccharides					530:544	seaweed polysaccharides agar, agarose, porphyran, κ-carrageenan, alginate and laminarin	522:608	seaweed polysaccharides agar, agarose, porphyran, κ-carrageenan, alginate and laminarin	522:608	Furthermore, strain S66T grew on seaweed polysaccharides agar, agarose, porphyran, κ-carrageenan, alginate and laminarin as sole carbon sources.
28671532	5	64	theme	seaweed	522:528	arg1	κ-carrageenan					572:584	κ-carrageenan	572:584	κ-carrageenan	572:584	Furthermore, strain S66T grew on seaweed polysaccharides agar, agarose, porphyran, κ-carrageenan, alginate and laminarin as sole carbon sources.
28671532	5	64	theme	seaweed	522:528	arg1	agar					546:549	agar	546:549	agar	546:549	Furthermore, strain S66T grew on seaweed polysaccharides agar, agarose, porphyran, κ-carrageenan, alginate and laminarin as sole carbon sources.
28671532	10	65	theme	other	1183:1187	arg1	members					1189:1195	other members	1183:1195	other members of the genus Paraglaciecola	1183:1223	The percentage similarity between the 16S rRNA gene and GyrB sequences of strain S66T and other members of the genus Paraglaciecola were 94-95 % and 84-85 %, respectively.
28671532	7	66	theme	respiratory	697:707	arg1	quinone					709:715	The respiratory quinone	693:715	The respiratory quinone	693:715	The respiratory quinone was determined to be Q-8, and major polar lipids were phosphatidylethanolamine and phosphatidylglycerol.
28671532	7	66	theme	respiratory	697:707	arg1	Q-8					738:740	Q-8	738:740	Q-8	738:740	The respiratory quinone was determined to be Q-8, and major polar lipids were phosphatidylethanolamine and phosphatidylglycerol.
28671532	12	67	theme	hydrolytica	1756:1766	arg1	sp					1768:1769	the name Paraglaciecola hydrolytica sp	1732:1769	the name Paraglaciecola hydrolytica sp	1732:1769	The phenotypic, phylogenetic and genomic analyses support the hypothesis that strain S66T represents a novel species of the genus Paraglaciecola, for which the name Paraglaciecola hydrolytica sp.
28671532	12	68	theme	genomic	1609:1615	arg1	analyses					1617:1624	The phenotypic, phylogenetic and genomic analyses	1576:1624	The phenotypic, phylogenetic and genomic analyses	1576:1624	The phenotypic, phylogenetic and genomic analyses support the hypothesis that strain S66T represents a novel species of the genus Paraglaciecola, for which the name Paraglaciecola hydrolytica sp.
28671532	8	69	theme	42.2 mol	863:870	arg1	content					834:840	The DNA G+C content	822:840	The DNA G+C content	822:840	The DNA G+C content was determined to be 42.2 mol%.
28671532	8	69	theme	42.2 mol	863:870	arg1	%					871:871	42.2 mol%	863:871	42.2 mol%	863:871	The DNA G+C content was determined to be 42.2 mol%.
28671532	12	70	theme	genus	1700:1704	arg1	Paraglaciecola					1706:1719	the genus Paraglaciecola	1696:1719	the genus Paraglaciecola	1696:1719	The phenotypic, phylogenetic and genomic analyses support the hypothesis that strain S66T represents a novel species of the genus Paraglaciecola, for which the name Paraglaciecola hydrolytica sp.
28671532	12	71	theme	Paraglaciecola	1741:1754	arg1	sp					1768:1769	the name Paraglaciecola hydrolytica sp	1732:1769	the name Paraglaciecola hydrolytica sp	1732:1769	The phenotypic, phylogenetic and genomic analyses support the hypothesis that strain S66T represents a novel species of the genus Paraglaciecola, for which the name Paraglaciecola hydrolytica sp.
28671532	4	72	theme	optimum	439:445	arg1	10-25 °C					429:436	10-25 °C	429:436	10-25 °C (optimum 20-25 °C)	429:455	The strain was Gram-reaction-negative, rod-shaped, aerobic, and displayed growth at 10-25 °C (optimum 20-25 °C) and at pH 7-9 (optimum pH 7.5).
28671532	4	72	theme	optimum	439:445	arg1	20-25 °C					447:454	optimum 20-25 °C	439:454	optimum 20-25 °C	439:454	The strain was Gram-reaction-negative, rod-shaped, aerobic, and displayed growth at 10-25 °C (optimum 20-25 °C) and at pH 7-9 (optimum pH 7.5).
28671532	14	73	theme	15060T=DSM	1833:1842	arg1	102834T					1844:1850	=LMG 29457T=NCIMB 15060T=DSM 102834T	1815:1850	=LMG 29457T=NCIMB 15060T=DSM 102834T	1815:1850	The type strain is S66T (=LMG 29457T=NCIMB 15060T=DSM 102834T).
28671532	14	73	theme	15060T=DSM	1833:1842	arg1	S66T					1809:1812	S66T	1809:1812	S66T (=LMG 29457T=NCIMB 15060T=DSM 102834T)	1809:1851	The type strain is S66T (=LMG 29457T=NCIMB 15060T=DSM 102834T).
28671532	8	74	theme	G+C	830:832	arg1	content					834:840	The DNA G+C content	822:840	The DNA G+C content	822:840	The DNA G+C content was determined to be 42.2 mol%.
28671532	8	74	theme	G+C	830:832	arg1	%					871:871	42.2 mol%	863:871	42.2 mol%	863:871	The DNA G+C content was determined to be 42.2 mol%.
28671532	10	75	theme	S66T	1174:1177	arg1	gene					1140:1143	the 16S rRNA gene and GyrB sequences	1127:1162	gene	1140:1143	The percentage similarity between the 16S rRNA gene and GyrB sequences of strain S66T and other members of the genus Paraglaciecola were 94-95 % and 84-85 %, respectively.
28671532	10	75	theme	S66T	1174:1177	arg1	sequences					1154:1162	the 16S rRNA gene and GyrB sequences	1127:1162	sequences	1154:1162	The percentage similarity between the 16S rRNA gene and GyrB sequences of strain S66T and other members of the genus Paraglaciecola were 94-95 % and 84-85 %, respectively.
28671532	10	76	theme	rRNA	1135:1138	arg1	gene					1140:1143	the 16S rRNA gene and GyrB sequences	1127:1162	gene	1140:1143	The percentage similarity between the 16S rRNA gene and GyrB sequences of strain S66T and other members of the genus Paraglaciecola were 94-95 % and 84-85 %, respectively.
28671532	14	77	theme	29457T=NCIMB	1820:1831	arg1	102834T					1844:1850	=LMG 29457T=NCIMB 15060T=DSM 102834T	1815:1850	=LMG 29457T=NCIMB 15060T=DSM 102834T	1815:1850	The type strain is S66T (=LMG 29457T=NCIMB 15060T=DSM 102834T).
28671532	14	77	theme	29457T=NCIMB	1820:1831	arg1	S66T					1809:1812	S66T	1809:1812	S66T (=LMG 29457T=NCIMB 15060T=DSM 102834T)	1809:1851	The type strain is S66T (=LMG 29457T=NCIMB 15060T=DSM 102834T).
28671532	12	78	theme	phenotypic	1580:1589	arg1	analyses					1617:1624	The phenotypic, phylogenetic and genomic analyses	1576:1624	The phenotypic, phylogenetic and genomic analyses	1576:1624	The phenotypic, phylogenetic and genomic analyses support the hypothesis that strain S66T represents a novel species of the genus Paraglaciecola, for which the name Paraglaciecola hydrolytica sp.
28671532	10	79	theme	strain	1167:1172	arg1	S66T					1174:1177	strain S66T	1167:1177	strain S66T	1167:1177	The percentage similarity between the 16S rRNA gene and GyrB sequences of strain S66T and other members of the genus Paraglaciecola were 94-95 % and 84-85 %, respectively.
28671532	10	80	theme	16S	1131:1133	arg1	gene					1140:1143	the 16S rRNA gene and GyrB sequences	1127:1162	gene	1140:1143	The percentage similarity between the 16S rRNA gene and GyrB sequences of strain S66T and other members of the genus Paraglaciecola were 94-95 % and 84-85 %, respectively.
28671532	1	81	with	bacterium	39:47	arg1	activity					65:72	hydrolytic activity	54:72	hydrolytic activity against multiple seaweed-derived polysaccharides	54:121	nov., a bacterium with hydrolytic activity against multiple seaweed-derived polysaccharides.
28671532	14	82	theme	=LMG	1815:1818	arg1	102834T					1844:1850	=LMG 29457T=NCIMB 15060T=DSM 102834T	1815:1850	=LMG 29457T=NCIMB 15060T=DSM 102834T	1815:1850	The type strain is S66T (=LMG 29457T=NCIMB 15060T=DSM 102834T).
28671532	14	82	theme	=LMG	1815:1818	arg1	S66T					1809:1812	S66T	1809:1812	S66T (=LMG 29457T=NCIMB 15060T=DSM 102834T)	1809:1851	The type strain is S66T (=LMG 29457T=NCIMB 15060T=DSM 102834T).
28671532	5	83	theme	strain	502:507	arg1	S66T					509:512	strain S66T	502:512	strain S66T	502:512	Furthermore, strain S66T grew on seaweed polysaccharides agar, agarose, porphyran, κ-carrageenan, alginate and laminarin as sole carbon sources.
28671532	10	84	theme	genus	1204:1208	arg1	Paraglaciecola					1210:1223	the genus Paraglaciecola	1200:1223	the genus Paraglaciecola	1200:1223	The percentage similarity between the 16S rRNA gene and GyrB sequences of strain S66T and other members of the genus Paraglaciecola were 94-95 % and 84-85 %, respectively.
28189223	5	0	theme	alleviating	1064:1074	arg1	radiation					1086:1094	alleviating secondary radiation	1064:1094	alleviating secondary radiation	1064:1094	Moreover, the shielding effectiveness due to absorption accounts for 78.2%-84.2% of SEtotal for FPCA, indicative of the absorption-dominant shielding mechanism contributing to alleviating secondary radiation.
28189223	4	1	theme	PPy	833:835	arg1	29.3dB					865:870	29.3dB	865:870	29.3dB	865:870	When utilized as electromagnetic interference (EMI) shielding materials, the α-Fe2O3/PPy/CDCA (FPCA) composite has the highest total shielding effectiveness (SEtotal) of 39.4dB, about 2.0, 2.9, and 1.3 times that of the acid-treated CDCA (19.3dB), PPy (13.6dB), and α-Fe2O3/CDCA (29.3dB), respectively.
28189223	4	1	theme	PPy	833:835	arg1	times					787:791	2.0, 2.9, and 1.3 times	769:791	2.0, 2.9, and 1.3 times	769:791	When utilized as electromagnetic interference (EMI) shielding materials, the α-Fe2O3/PPy/CDCA (FPCA) composite has the highest total shielding effectiveness (SEtotal) of 39.4dB, about 2.0, 2.9, and 1.3 times that of the acid-treated CDCA (19.3dB), PPy (13.6dB), and α-Fe2O3/CDCA (29.3dB), respectively.
28189223	4	2	theme	total	712:716	arg1	effectiveness					728:740	the highest total shielding effectiveness	700:740	the highest total shielding effectiveness (SEtotal) of 39.4dB	700:760	When utilized as electromagnetic interference (EMI) shielding materials, the α-Fe2O3/PPy/CDCA (FPCA) composite has the highest total shielding effectiveness (SEtotal) of 39.4dB, about 2.0, 2.9, and 1.3 times that of the acid-treated CDCA (19.3dB), PPy (13.6dB), and α-Fe2O3/CDCA (29.3dB), respectively.
28189223	4	2	theme	total	712:716	arg1	SEtotal					743:749	SEtotal	743:749	SEtotal	743:749	When utilized as electromagnetic interference (EMI) shielding materials, the α-Fe2O3/PPy/CDCA (FPCA) composite has the highest total shielding effectiveness (SEtotal) of 39.4dB, about 2.0, 2.9, and 1.3 times that of the acid-treated CDCA (19.3dB), PPy (13.6dB), and α-Fe2O3/CDCA (29.3dB), respectively.
28189223	4	3	contain	has	696:698	arg2	SEtotal					743:749	SEtotal	743:749	SEtotal	743:749	When utilized as electromagnetic interference (EMI) shielding materials, the α-Fe2O3/PPy/CDCA (FPCA) composite has the highest total shielding effectiveness (SEtotal) of 39.4dB, about 2.0, 2.9, and 1.3 times that of the acid-treated CDCA (19.3dB), PPy (13.6dB), and α-Fe2O3/CDCA (29.3dB), respectively.
28189223	4	3	contain	has	696:698	arg2	effectiveness					728:740	the highest total shielding effectiveness	700:740	the highest total shielding effectiveness (SEtotal) of 39.4dB	700:760	When utilized as electromagnetic interference (EMI) shielding materials, the α-Fe2O3/PPy/CDCA (FPCA) composite has the highest total shielding effectiveness (SEtotal) of 39.4dB, about 2.0, 2.9, and 1.3 times that of the acid-treated CDCA (19.3dB), PPy (13.6dB), and α-Fe2O3/CDCA (29.3dB), respectively.
28189223	4	3	contain	has	696:698	arg1	composite					686:694	the α-Fe2O3/PPy/CDCA (FPCA) composite	658:694	the α-Fe2O3/PPy/CDCA (FPCA) composite	658:694	When utilized as electromagnetic interference (EMI) shielding materials, the α-Fe2O3/PPy/CDCA (FPCA) composite has the highest total shielding effectiveness (SEtotal) of 39.4dB, about 2.0, 2.9, and 1.3 times that of the acid-treated CDCA (19.3dB), PPy (13.6dB), and α-Fe2O3/CDCA (29.3dB), respectively.
28189223	4	4	theme	highest	704:710	arg1	effectiveness					728:740	the highest total shielding effectiveness	700:740	the highest total shielding effectiveness (SEtotal) of 39.4dB	700:760	When utilized as electromagnetic interference (EMI) shielding materials, the α-Fe2O3/PPy/CDCA (FPCA) composite has the highest total shielding effectiveness (SEtotal) of 39.4dB, about 2.0, 2.9, and 1.3 times that of the acid-treated CDCA (19.3dB), PPy (13.6dB), and α-Fe2O3/CDCA (29.3dB), respectively.
28189223	4	4	theme	highest	704:710	arg1	SEtotal					743:749	SEtotal	743:749	SEtotal	743:749	When utilized as electromagnetic interference (EMI) shielding materials, the α-Fe2O3/PPy/CDCA (FPCA) composite has the highest total shielding effectiveness (SEtotal) of 39.4dB, about 2.0, 2.9, and 1.3 times that of the acid-treated CDCA (19.3dB), PPy (13.6dB), and α-Fe2O3/CDCA (29.3dB), respectively.
28189223	2	5	theme	SEM	321:323	arg1	observations					333:344	The SEM and TEM observations	317:344	The SEM and TEM observations	317:344	The SEM and TEM observations present that the wrinkled PPy sheets and the α-Fe2O3 nanoparticles were well dispersed in CDCA.
28189223	4	6	theme	39.4dB	755:760	arg1	effectiveness					728:740	the highest total shielding effectiveness	700:740	the highest total shielding effectiveness (SEtotal) of 39.4dB	700:760	When utilized as electromagnetic interference (EMI) shielding materials, the α-Fe2O3/PPy/CDCA (FPCA) composite has the highest total shielding effectiveness (SEtotal) of 39.4dB, about 2.0, 2.9, and 1.3 times that of the acid-treated CDCA (19.3dB), PPy (13.6dB), and α-Fe2O3/CDCA (29.3dB), respectively.
28189223	4	6	theme	39.4dB	755:760	arg1	SEtotal					743:749	SEtotal	743:749	SEtotal	743:749	When utilized as electromagnetic interference (EMI) shielding materials, the α-Fe2O3/PPy/CDCA (FPCA) composite has the highest total shielding effectiveness (SEtotal) of 39.4dB, about 2.0, 2.9, and 1.3 times that of the acid-treated CDCA (19.3dB), PPy (13.6dB), and α-Fe2O3/CDCA (29.3dB), respectively.
28189223	4	7	theme	interference	618:629	arg1	materials					647:655	electromagnetic interference (EMI) shielding materials	602:655	electromagnetic interference (EMI) shielding materials	602:655	When utilized as electromagnetic interference (EMI) shielding materials, the α-Fe2O3/PPy/CDCA (FPCA) composite has the highest total shielding effectiveness (SEtotal) of 39.4dB, about 2.0, 2.9, and 1.3 times that of the acid-treated CDCA (19.3dB), PPy (13.6dB), and α-Fe2O3/CDCA (29.3dB), respectively.
28189223	5	8	theme	absorption-dominant	1008:1026	arg1	mechanism					1038:1046	the absorption-dominant shielding mechanism	1004:1046	the absorption-dominant shielding mechanism contributing to alleviating secondary radiation	1004:1094	Moreover, the shielding effectiveness due to absorption accounts for 78.2%-84.2% of SEtotal for FPCA, indicative of the absorption-dominant shielding mechanism contributing to alleviating secondary radiation.
28189223	5	9	theme	shielding	902:910	arg1	effectiveness					912:924	the shielding effectiveness	898:924	the shielding effectiveness due to absorption	898:942	Moreover, the shielding effectiveness due to absorption accounts for 78.2%-84.2% of SEtotal for FPCA, indicative of the absorption-dominant shielding mechanism contributing to alleviating secondary radiation.
28189223	1	10	theme	vapor-phase	289:299	arg1	polymerization					301:314	vapor-phase polymerization	289:314	vapor-phase polymerization	289:314	Eco-friendly cellulose-derived carbon aerogels (CDCA) were employed as porous substrate to integrate with α-Fe2O3 and polypyrrole (PPy) via pyrolysis and vapor-phase polymerization.
28189223	2	11	theme	PPy	372:374	arg1	sheets					376:381	the wrinkled PPy sheets	359:381	the wrinkled PPy sheets	359:381	The SEM and TEM observations present that the wrinkled PPy sheets and the α-Fe2O3 nanoparticles were well dispersed in CDCA.
28189223	4	12	theme	FPCA	680:683	arg1	composite					686:694	the α-Fe2O3/PPy/CDCA (FPCA) composite	658:694	the α-Fe2O3/PPy/CDCA (FPCA) composite	658:694	When utilized as electromagnetic interference (EMI) shielding materials, the α-Fe2O3/PPy/CDCA (FPCA) composite has the highest total shielding effectiveness (SEtotal) of 39.4dB, about 2.0, 2.9, and 1.3 times that of the acid-treated CDCA (19.3dB), PPy (13.6dB), and α-Fe2O3/CDCA (29.3dB), respectively.
28189223	4	13	theme	electromagnetic	602:616	arg1	materials					647:655	electromagnetic interference (EMI) shielding materials	602:655	electromagnetic interference (EMI) shielding materials	602:655	When utilized as electromagnetic interference (EMI) shielding materials, the α-Fe2O3/PPy/CDCA (FPCA) composite has the highest total shielding effectiveness (SEtotal) of 39.4dB, about 2.0, 2.9, and 1.3 times that of the acid-treated CDCA (19.3dB), PPy (13.6dB), and α-Fe2O3/CDCA (29.3dB), respectively.
28189223	5	14	theme	mechanism	1038:1046	arg1	indicative					990:999	indicative	990:999	indicative	990:999	Moreover, the shielding effectiveness due to absorption accounts for 78.2%-84.2% of SEtotal for FPCA, indicative of the absorption-dominant shielding mechanism contributing to alleviating secondary radiation.
28189223	4	15	theme	CDCA	818:821	arg1	29.3dB					865:870	29.3dB	865:870	29.3dB	865:870	When utilized as electromagnetic interference (EMI) shielding materials, the α-Fe2O3/PPy/CDCA (FPCA) composite has the highest total shielding effectiveness (SEtotal) of 39.4dB, about 2.0, 2.9, and 1.3 times that of the acid-treated CDCA (19.3dB), PPy (13.6dB), and α-Fe2O3/CDCA (29.3dB), respectively.
28189223	4	15	theme	CDCA	818:821	arg1	times					787:791	2.0, 2.9, and 1.3 times	769:791	2.0, 2.9, and 1.3 times	769:791	When utilized as electromagnetic interference (EMI) shielding materials, the α-Fe2O3/PPy/CDCA (FPCA) composite has the highest total shielding effectiveness (SEtotal) of 39.4dB, about 2.0, 2.9, and 1.3 times that of the acid-treated CDCA (19.3dB), PPy (13.6dB), and α-Fe2O3/CDCA (29.3dB), respectively.
28189223	1	16	theme	porous	206:211	arg1	substrate					213:221	porous substrate	206:221	porous substrate	206:221	Eco-friendly cellulose-derived carbon aerogels (CDCA) were employed as porous substrate to integrate with α-Fe2O3 and polypyrrole (PPy) via pyrolysis and vapor-phase polymerization.
28189223	1	16	theme	porous	206:211	arg1	aerogels					173:180	Eco-friendly cellulose-derived carbon aerogels	135:180	Eco-friendly cellulose-derived carbon aerogels (CDCA)	135:187	Eco-friendly cellulose-derived carbon aerogels (CDCA) were employed as porous substrate to integrate with α-Fe2O3 and polypyrrole (PPy) via pyrolysis and vapor-phase polymerization.
28189223	0	17	link	cellulose-derived	56:72	arg1	aerogels					81:88	cellulose-derived carbon aerogels	56:88	cellulose-derived carbon aerogels	56:88	Synthesis and electromagnetic interference shielding of cellulose-derived carbon aerogels functionalized with α-Fe2O3 and polypyrrole.
28189223	5	18	theme	shielding	1028:1036	arg1	mechanism					1038:1046	the absorption-dominant shielding mechanism	1004:1046	the absorption-dominant shielding mechanism contributing to alleviating secondary radiation	1004:1094	Moreover, the shielding effectiveness due to absorption accounts for 78.2%-84.2% of SEtotal for FPCA, indicative of the absorption-dominant shielding mechanism contributing to alleviating secondary radiation.
28189223	3	19	theme	strong	446:451	arg1	interactions					453:464	The strong interactions	442:464	The strong interactions (such as hydrogen bonding) between the substrate and the nanomaterials	442:535	The strong interactions (such as hydrogen bonding) between the substrate and the nanomaterials were demonstrated by the FTIR and XPS analysis.
28189223	5	20	theme	secondary	1076:1084	arg1	radiation					1086:1094	alleviating secondary radiation	1064:1094	alleviating secondary radiation	1064:1094	Moreover, the shielding effectiveness due to absorption accounts for 78.2%-84.2% of SEtotal for FPCA, indicative of the absorption-dominant shielding mechanism contributing to alleviating secondary radiation.
28189223	0	21	theme	interference	30:41	arg1	shielding					43:51	electromagnetic interference shielding	14:51	electromagnetic interference shielding	14:51	Synthesis and electromagnetic interference shielding of cellulose-derived carbon aerogels functionalized with α-Fe2O3 and polypyrrole.
28189223	3	22	theme	XPS	571:573	arg1	analysis					575:582	the FTIR and XPS analysis	558:582	analysis	575:582	The strong interactions (such as hydrogen bonding) between the substrate and the nanomaterials were demonstrated by the FTIR and XPS analysis.
28189223	0	23	theme	electromagnetic	14:28	arg1	shielding					43:51	electromagnetic interference shielding	14:51	electromagnetic interference shielding	14:51	Synthesis and electromagnetic interference shielding of cellulose-derived carbon aerogels functionalized with α-Fe2O3 and polypyrrole.
28189223	6	24	theme	useful	1133:1138	arg1	candidate					1152:1160	a useful alternative candidate	1131:1160	a useful alternative candidate for EMI shielding	1131:1178	These features make the composite a useful alternative candidate for EMI shielding.
28189223	1	25	theme	Eco-friendly	135:146	arg1	aerogels					173:180	Eco-friendly cellulose-derived carbon aerogels	135:180	Eco-friendly cellulose-derived carbon aerogels (CDCA)	135:187	Eco-friendly cellulose-derived carbon aerogels (CDCA) were employed as porous substrate to integrate with α-Fe2O3 and polypyrrole (PPy) via pyrolysis and vapor-phase polymerization.
28189223	1	25	theme	Eco-friendly	135:146	arg1	substrate					213:221	porous substrate	206:221	porous substrate	206:221	Eco-friendly cellulose-derived carbon aerogels (CDCA) were employed as porous substrate to integrate with α-Fe2O3 and polypyrrole (PPy) via pyrolysis and vapor-phase polymerization.
28189223	1	25	theme	Eco-friendly	135:146	arg1	CDCA					183:186	CDCA	183:186	CDCA	183:186	Eco-friendly cellulose-derived carbon aerogels (CDCA) were employed as porous substrate to integrate with α-Fe2O3 and polypyrrole (PPy) via pyrolysis and vapor-phase polymerization.
28189223	2	26	theme	wrinkled	363:370	arg1	sheets					376:381	the wrinkled PPy sheets	359:381	the wrinkled PPy sheets	359:381	The SEM and TEM observations present that the wrinkled PPy sheets and the α-Fe2O3 nanoparticles were well dispersed in CDCA.
28189223	5	27	theme	indicative	990:999	arg1	FPCA					984:987	FPCA	984:987	FPCA	984:987	Moreover, the shielding effectiveness due to absorption accounts for 78.2%-84.2% of SEtotal for FPCA, indicative of the absorption-dominant shielding mechanism contributing to alleviating secondary radiation.
28189223	4	28	theme	α-Fe2O3/PPy/CDCA	662:677	arg1	composite					686:694	the α-Fe2O3/PPy/CDCA (FPCA) composite	658:694	the α-Fe2O3/PPy/CDCA (FPCA) composite	658:694	When utilized as electromagnetic interference (EMI) shielding materials, the α-Fe2O3/PPy/CDCA (FPCA) composite has the highest total shielding effectiveness (SEtotal) of 39.4dB, about 2.0, 2.9, and 1.3 times that of the acid-treated CDCA (19.3dB), PPy (13.6dB), and α-Fe2O3/CDCA (29.3dB), respectively.
28189223	4	29	dep	times	787:791	arg1	that					793:796	that	793:796	that	793:796	When utilized as electromagnetic interference (EMI) shielding materials, the α-Fe2O3/PPy/CDCA (FPCA) composite has the highest total shielding effectiveness (SEtotal) of 39.4dB, about 2.0, 2.9, and 1.3 times that of the acid-treated CDCA (19.3dB), PPy (13.6dB), and α-Fe2O3/CDCA (29.3dB), respectively.
28189223	3	30	dep	interactions	453:464	arg1	such					467:470	such	467:470	such	467:470	The strong interactions (such as hydrogen bonding) between the substrate and the nanomaterials were demonstrated by the FTIR and XPS analysis.
28189223	3	31	theme	hydrogen	475:482	arg1	bonding					484:490	hydrogen bonding	475:490	hydrogen bonding	475:490	The strong interactions (such as hydrogen bonding) between the substrate and the nanomaterials were demonstrated by the FTIR and XPS analysis.
28189223	0	32	theme	carbon	74:79	arg1	aerogels					81:88	cellulose-derived carbon aerogels	56:88	cellulose-derived carbon aerogels	56:88	Synthesis and electromagnetic interference shielding of cellulose-derived carbon aerogels functionalized with α-Fe2O3 and polypyrrole.
28189223	4	33	theme	acid-treated	805:816	arg1	19.3dB					824:829	19.3dB	824:829	19.3dB	824:829	When utilized as electromagnetic interference (EMI) shielding materials, the α-Fe2O3/PPy/CDCA (FPCA) composite has the highest total shielding effectiveness (SEtotal) of 39.4dB, about 2.0, 2.9, and 1.3 times that of the acid-treated CDCA (19.3dB), PPy (13.6dB), and α-Fe2O3/CDCA (29.3dB), respectively.
28189223	4	33	theme	acid-treated	805:816	arg1	CDCA					818:821	the acid-treated CDCA	801:821	the acid-treated CDCA (19.3dB)	801:830	When utilized as electromagnetic interference (EMI) shielding materials, the α-Fe2O3/PPy/CDCA (FPCA) composite has the highest total shielding effectiveness (SEtotal) of 39.4dB, about 2.0, 2.9, and 1.3 times that of the acid-treated CDCA (19.3dB), PPy (13.6dB), and α-Fe2O3/CDCA (29.3dB), respectively.
28189223	4	34	theme	α-Fe2O3/CDCA	851:862	arg1	29.3dB					865:870	29.3dB	865:870	29.3dB	865:870	When utilized as electromagnetic interference (EMI) shielding materials, the α-Fe2O3/PPy/CDCA (FPCA) composite has the highest total shielding effectiveness (SEtotal) of 39.4dB, about 2.0, 2.9, and 1.3 times that of the acid-treated CDCA (19.3dB), PPy (13.6dB), and α-Fe2O3/CDCA (29.3dB), respectively.
28189223	4	34	theme	α-Fe2O3/CDCA	851:862	arg1	times					787:791	2.0, 2.9, and 1.3 times	769:791	2.0, 2.9, and 1.3 times	769:791	When utilized as electromagnetic interference (EMI) shielding materials, the α-Fe2O3/PPy/CDCA (FPCA) composite has the highest total shielding effectiveness (SEtotal) of 39.4dB, about 2.0, 2.9, and 1.3 times that of the acid-treated CDCA (19.3dB), PPy (13.6dB), and α-Fe2O3/CDCA (29.3dB), respectively.
28189223	1	35	theme	cellulose-derived	148:164	arg1	aerogels					173:180	Eco-friendly cellulose-derived carbon aerogels	135:180	Eco-friendly cellulose-derived carbon aerogels (CDCA)	135:187	Eco-friendly cellulose-derived carbon aerogels (CDCA) were employed as porous substrate to integrate with α-Fe2O3 and polypyrrole (PPy) via pyrolysis and vapor-phase polymerization.
28189223	1	35	theme	cellulose-derived	148:164	arg1	substrate					213:221	porous substrate	206:221	porous substrate	206:221	Eco-friendly cellulose-derived carbon aerogels (CDCA) were employed as porous substrate to integrate with α-Fe2O3 and polypyrrole (PPy) via pyrolysis and vapor-phase polymerization.
28189223	1	35	theme	cellulose-derived	148:164	arg1	CDCA					183:186	CDCA	183:186	CDCA	183:186	Eco-friendly cellulose-derived carbon aerogels (CDCA) were employed as porous substrate to integrate with α-Fe2O3 and polypyrrole (PPy) via pyrolysis and vapor-phase polymerization.
28189223	0	36	theme	cellulose-derived	56:72	arg1	aerogels					81:88	cellulose-derived carbon aerogels	56:88	cellulose-derived carbon aerogels	56:88	Synthesis and electromagnetic interference shielding of cellulose-derived carbon aerogels functionalized with α-Fe2O3 and polypyrrole.
28189223	1	37	theme	carbon	166:171	arg1	aerogels					173:180	Eco-friendly cellulose-derived carbon aerogels	135:180	Eco-friendly cellulose-derived carbon aerogels (CDCA)	135:187	Eco-friendly cellulose-derived carbon aerogels (CDCA) were employed as porous substrate to integrate with α-Fe2O3 and polypyrrole (PPy) via pyrolysis and vapor-phase polymerization.
28189223	1	37	theme	carbon	166:171	arg1	substrate					213:221	porous substrate	206:221	porous substrate	206:221	Eco-friendly cellulose-derived carbon aerogels (CDCA) were employed as porous substrate to integrate with α-Fe2O3 and polypyrrole (PPy) via pyrolysis and vapor-phase polymerization.
28189223	1	37	theme	carbon	166:171	arg1	CDCA					183:186	CDCA	183:186	CDCA	183:186	Eco-friendly cellulose-derived carbon aerogels (CDCA) were employed as porous substrate to integrate with α-Fe2O3 and polypyrrole (PPy) via pyrolysis and vapor-phase polymerization.
28189223	1	38	link	cellulose-derived	148:164	arg1	aerogels					173:180	Eco-friendly cellulose-derived carbon aerogels	135:180	Eco-friendly cellulose-derived carbon aerogels (CDCA)	135:187	Eco-friendly cellulose-derived carbon aerogels (CDCA) were employed as porous substrate to integrate with α-Fe2O3 and polypyrrole (PPy) via pyrolysis and vapor-phase polymerization.
28189223	1	38	link	cellulose-derived	148:164	arg1	substrate					213:221	porous substrate	206:221	porous substrate	206:221	Eco-friendly cellulose-derived carbon aerogels (CDCA) were employed as porous substrate to integrate with α-Fe2O3 and polypyrrole (PPy) via pyrolysis and vapor-phase polymerization.
28189223	1	38	link	cellulose-derived	148:164	arg1	CDCA					183:186	CDCA	183:186	CDCA	183:186	Eco-friendly cellulose-derived carbon aerogels (CDCA) were employed as porous substrate to integrate with α-Fe2O3 and polypyrrole (PPy) via pyrolysis and vapor-phase polymerization.
28189223	5	39	theme	SEtotal	972:978	arg1	SEtotal					972:978	SEtotal	972:978	SEtotal	972:978	Moreover, the shielding effectiveness due to absorption accounts for 78.2%-84.2% of SEtotal for FPCA, indicative of the absorption-dominant shielding mechanism contributing to alleviating secondary radiation.
28189223	5	39	theme	SEtotal	972:978	arg1	%					967:967	78.2%-84.2%	957:967	78.2%-84.2% of SEtotal	957:978	Moreover, the shielding effectiveness due to absorption accounts for 78.2%-84.2% of SEtotal for FPCA, indicative of the absorption-dominant shielding mechanism contributing to alleviating secondary radiation.
28189223	6	40	theme	EMI	1166:1168	arg1	shielding					1170:1178	EMI shielding	1166:1178	EMI shielding	1166:1178	These features make the composite a useful alternative candidate for EMI shielding.
28189223	5	41	theme	%	961:961	arg1	SEtotal					972:978	SEtotal	972:978	SEtotal	972:978	Moreover, the shielding effectiveness due to absorption accounts for 78.2%-84.2% of SEtotal for FPCA, indicative of the absorption-dominant shielding mechanism contributing to alleviating secondary radiation.
28189223	5	41	theme	%	961:961	arg1	%					967:967	78.2%-84.2%	957:967	78.2%-84.2% of SEtotal	957:978	Moreover, the shielding effectiveness due to absorption accounts for 78.2%-84.2% of SEtotal for FPCA, indicative of the absorption-dominant shielding mechanism contributing to alleviating secondary radiation.
28189223	2	42	theme	TEM	329:331	arg1	observations					333:344	The SEM and TEM observations	317:344	The SEM and TEM observations	317:344	The SEM and TEM observations present that the wrinkled PPy sheets and the α-Fe2O3 nanoparticles were well dispersed in CDCA.
28189223	4	43	theme	EMI	632:634	arg1	materials					647:655	electromagnetic interference (EMI) shielding materials	602:655	electromagnetic interference (EMI) shielding materials	602:655	When utilized as electromagnetic interference (EMI) shielding materials, the α-Fe2O3/PPy/CDCA (FPCA) composite has the highest total shielding effectiveness (SEtotal) of 39.4dB, about 2.0, 2.9, and 1.3 times that of the acid-treated CDCA (19.3dB), PPy (13.6dB), and α-Fe2O3/CDCA (29.3dB), respectively.
28189223	3	44	theme	FTIR	562:565	arg1	analysis					575:582	the FTIR and XPS analysis	558:582	analysis	575:582	The strong interactions (such as hydrogen bonding) between the substrate and the nanomaterials were demonstrated by the FTIR and XPS analysis.
28189223	2	45	theme	α-Fe2O3	391:397	arg1	nanoparticles					399:411	the α-Fe2O3 nanoparticles	387:411	the α-Fe2O3 nanoparticles	387:411	The SEM and TEM observations present that the wrinkled PPy sheets and the α-Fe2O3 nanoparticles were well dispersed in CDCA.
28189223	4	46	theme	shielding	637:645	arg1	materials					647:655	electromagnetic interference (EMI) shielding materials	602:655	electromagnetic interference (EMI) shielding materials	602:655	When utilized as electromagnetic interference (EMI) shielding materials, the α-Fe2O3/PPy/CDCA (FPCA) composite has the highest total shielding effectiveness (SEtotal) of 39.4dB, about 2.0, 2.9, and 1.3 times that of the acid-treated CDCA (19.3dB), PPy (13.6dB), and α-Fe2O3/CDCA (29.3dB), respectively.
28189223	0	47	theme	aerogels	81:88	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis and electromagnetic interference shielding of cellulose-derived carbon aerogels functionalized with α-Fe2O3 and polypyrrole.
28189223	0	47	theme	aerogels	81:88	arg1	shielding					43:51	electromagnetic interference shielding	14:51	electromagnetic interference shielding	14:51	Synthesis and electromagnetic interference shielding of cellulose-derived carbon aerogels functionalized with α-Fe2O3 and polypyrrole.
28189223	6	48	theme	alternative	1140:1150	arg1	candidate					1152:1160	a useful alternative candidate	1131:1160	a useful alternative candidate for EMI shielding	1131:1178	These features make the composite a useful alternative candidate for EMI shielding.
28189223	4	49	theme	shielding	718:726	arg1	effectiveness					728:740	the highest total shielding effectiveness	700:740	the highest total shielding effectiveness (SEtotal) of 39.4dB	700:760	When utilized as electromagnetic interference (EMI) shielding materials, the α-Fe2O3/PPy/CDCA (FPCA) composite has the highest total shielding effectiveness (SEtotal) of 39.4dB, about 2.0, 2.9, and 1.3 times that of the acid-treated CDCA (19.3dB), PPy (13.6dB), and α-Fe2O3/CDCA (29.3dB), respectively.
28189223	4	49	theme	shielding	718:726	arg1	SEtotal					743:749	SEtotal	743:749	SEtotal	743:749	When utilized as electromagnetic interference (EMI) shielding materials, the α-Fe2O3/PPy/CDCA (FPCA) composite has the highest total shielding effectiveness (SEtotal) of 39.4dB, about 2.0, 2.9, and 1.3 times that of the acid-treated CDCA (19.3dB), PPy (13.6dB), and α-Fe2O3/CDCA (29.3dB), respectively.
26159674	5	0	theme	constant	755:762	arg1	range					778:782	a constant concentration range	753:782	a constant concentration range	753:782	The results showed that the released nano-silver was intelligently maintained within a constant concentration range, so that it could be further designed to exhibit antimicrobial activity without cytotoxicity.
26159674	1	1	theme	Alg	248:250	arg1	gel					253:255	dehydrated alginate (Alg) gel	227:255	dehydrated alginate (Alg) gel	227:255	In this study, a novel intelligent antimicrobial model was constructed based on the antibiotic properties of nano-silver and the ion-exchange response of dehydrated alginate (Alg) gel.
26159674	5	2	theme	concentration	764:776	arg1	range					778:782	a constant concentration range	753:782	a constant concentration range	753:782	The results showed that the released nano-silver was intelligently maintained within a constant concentration range, so that it could be further designed to exhibit antimicrobial activity without cytotoxicity.
26159674	6	3	theme	infection	908:916	arg1	model					918:922	the murine wound infection model	891:922	the murine wound infection model conducted with these composites	891:954	Furthermore, the murine wound infection model conducted with these composites resulted in a significant decrease of bacteria number.
26159674	6	4	theme	number	1003:1008	arg1	decrease					982:989	a significant decrease	968:989	a significant decrease of bacteria number	968:1008	Furthermore, the murine wound infection model conducted with these composites resulted in a significant decrease of bacteria number.
26159674	6	5	theme	wound	902:906	arg1	model					918:922	the murine wound infection model	891:922	the murine wound infection model conducted with these composites	891:954	Furthermore, the murine wound infection model conducted with these composites resulted in a significant decrease of bacteria number.
26159674	1	6	theme	gel	253:255	arg1	nano-silver					182:192	nano-silver	182:192	nano-silver	182:192	In this study, a novel intelligent antimicrobial model was constructed based on the antibiotic properties of nano-silver and the ion-exchange response of dehydrated alginate (Alg) gel.
26159674	1	6	theme	gel	253:255	arg1	response					215:222	the ion-exchange response	198:222	the ion-exchange response of dehydrated alginate (Alg) gel	198:255	In this study, a novel intelligent antimicrobial model was constructed based on the antibiotic properties of nano-silver and the ion-exchange response of dehydrated alginate (Alg) gel.
26159674	6	7	theme	murine	895:900	arg1	model					918:922	the murine wound infection model	891:922	the murine wound infection model conducted with these composites	891:954	Furthermore, the murine wound infection model conducted with these composites resulted in a significant decrease of bacteria number.
26159674	7	8	theme	nano-silver	1127:1137	arg1	release					1116:1122	the controlled release	1101:1122	Alg along with the controlled release of nano-silver	1086:1137	The self-regulating swelling feature based on the ion-exchange response of Alg along with the controlled release of nano-silver made this composite a promising intelligent model for antimicrobial wound dressing applications.
26159674	7	9	theme	dressing	1213:1220	arg1	applications					1222:1233	antimicrobial wound dressing applications	1193:1233	antimicrobial wound dressing applications	1193:1233	The self-regulating swelling feature based on the ion-exchange response of Alg along with the controlled release of nano-silver made this composite a promising intelligent model for antimicrobial wound dressing applications.
26159674	7	10	theme	controlled	1105:1114	arg1	release					1116:1122	the controlled release	1101:1122	Alg along with the controlled release of nano-silver	1086:1137	The self-regulating swelling feature based on the ion-exchange response of Alg along with the controlled release of nano-silver made this composite a promising intelligent model for antimicrobial wound dressing applications.
26159674	1	11	theme	antibiotic	157:166	arg1	properties					168:177	the antibiotic properties	153:177	the antibiotic properties of nano-silver and the ion-exchange response of dehydrated alginate (Alg) gel	153:255	In this study, a novel intelligent antimicrobial model was constructed based on the antibiotic properties of nano-silver and the ion-exchange response of dehydrated alginate (Alg) gel.
26159674	7	12	theme	composite	1149:1157	arg1	model					1183:1187	this composite a promising intelligent model	1144:1187	this composite a promising intelligent model for antimicrobial wound dressing applications	1144:1233	The self-regulating swelling feature based on the ion-exchange response of Alg along with the controlled release of nano-silver made this composite a promising intelligent model for antimicrobial wound dressing applications.
26159674	4	13	theme	outside-in	564:573	arg1	swelling					575:582	the outside-in swelling	560:582	the outside-in swelling	560:582	In this model, the releasing level of nano-silver is determined by the outside-in swelling of Alg composites, which is further self-regulated by the volume of wound exudates.
26159674	4	14	theme	Alg	587:589	arg1	composites					591:600	Alg composites	587:600	Alg composites	587:600	In this model, the releasing level of nano-silver is determined by the outside-in swelling of Alg composites, which is further self-regulated by the volume of wound exudates.
26159674	3	15	theme	model	428:432	arg1	feature					412:418	The distinguished feature	394:418	The distinguished feature of this model	394:432	The distinguished feature of this model lies in its antimicrobial properties and biocompatibility.
26159674	0	16	theme	self-regulating	2:16	arg1	model					32:36	A self-regulating antimicrobial model	0:36	A self-regulating antimicrobial model	0:36	A self-regulating antimicrobial model based on the ion-exchange stimuli.
26159674	7	17	dep	along	1090:1094	arg1	with					1096:1099	with	1096:1099	with	1096:1099	The self-regulating swelling feature based on the ion-exchange response of Alg along with the controlled release of nano-silver made this composite a promising intelligent model for antimicrobial wound dressing applications.
26159674	2	18	theme	hydrogel	300:307	arg1	reaction					290:297	reaction	290:297	reaction	290:297	Through the process of reducing reaction, hydrogel formation and dehydration, the model composed of Alg and nano-silver was fabricated.
26159674	2	18	theme	hydrogel	300:307	arg1	formation					309:317	hydrogel formation	300:317	hydrogel formation	300:317	Through the process of reducing reaction, hydrogel formation and dehydration, the model composed of Alg and nano-silver was fabricated.
26159674	7	19	theme	antimicrobial	1193:1205	arg1	applications					1222:1233	antimicrobial wound dressing applications	1193:1233	antimicrobial wound dressing applications	1193:1233	The self-regulating swelling feature based on the ion-exchange response of Alg along with the controlled release of nano-silver made this composite a promising intelligent model for antimicrobial wound dressing applications.
26159674	4	20	theme	wound	652:656	arg1	exudates					658:665	wound exudates	652:665	wound exudates	652:665	In this model, the releasing level of nano-silver is determined by the outside-in swelling of Alg composites, which is further self-regulated by the volume of wound exudates.
26159674	7	21	theme	wound	1207:1211	arg1	applications					1222:1233	antimicrobial wound dressing applications	1193:1233	antimicrobial wound dressing applications	1193:1233	The self-regulating swelling feature based on the ion-exchange response of Alg along with the controlled release of nano-silver made this composite a promising intelligent model for antimicrobial wound dressing applications.
26159674	7	22	theme	intelligent	1171:1181	arg1	model					1183:1187	this composite a promising intelligent model	1144:1187	this composite a promising intelligent model for antimicrobial wound dressing applications	1144:1233	The self-regulating swelling feature based on the ion-exchange response of Alg along with the controlled release of nano-silver made this composite a promising intelligent model for antimicrobial wound dressing applications.
26159674	1	23	theme	nano-silver	182:192	arg1	properties					168:177	the antibiotic properties	153:177	the antibiotic properties of nano-silver and the ion-exchange response of dehydrated alginate (Alg) gel	153:255	In this study, a novel intelligent antimicrobial model was constructed based on the antibiotic properties of nano-silver and the ion-exchange response of dehydrated alginate (Alg) gel.
26159674	4	24	theme	nano-silver	531:541	arg1	level					522:526	the releasing level	508:526	the releasing level of nano-silver	508:541	In this model, the releasing level of nano-silver is determined by the outside-in swelling of Alg composites, which is further self-regulated by the volume of wound exudates.
26159674	7	25	theme	Alg	1086:1088	arg1	response					1074:1081	the ion-exchange response	1057:1081	the ion-exchange response of Alg along with the controlled release of nano-silver	1057:1137	The self-regulating swelling feature based on the ion-exchange response of Alg along with the controlled release of nano-silver made this composite a promising intelligent model for antimicrobial wound dressing applications.
26159674	0	26	theme	antimicrobial	18:30	arg1	model					32:36	A self-regulating antimicrobial model	0:36	A self-regulating antimicrobial model	0:36	A self-regulating antimicrobial model based on the ion-exchange stimuli.
26159674	1	27	theme	novel	90:94	arg1	model					122:126	a novel intelligent antimicrobial model	88:126	a novel intelligent antimicrobial model	88:126	In this study, a novel intelligent antimicrobial model was constructed based on the antibiotic properties of nano-silver and the ion-exchange response of dehydrated alginate (Alg) gel.
26159674	3	28	theme	antimicrobial	446:458	arg1	properties					460:469	its antimicrobial properties	442:469	its antimicrobial properties	442:469	The distinguished feature of this model lies in its antimicrobial properties and biocompatibility.
26159674	7	29	theme	promising	1161:1169	arg1	model					1183:1187	this composite a promising intelligent model	1144:1187	this composite a promising intelligent model for antimicrobial wound dressing applications	1144:1233	The self-regulating swelling feature based on the ion-exchange response of Alg along with the controlled release of nano-silver made this composite a promising intelligent model for antimicrobial wound dressing applications.
26159674	1	30	theme	ion-exchange	202:213	arg1	response					215:222	the ion-exchange response	198:222	the ion-exchange response of dehydrated alginate (Alg) gel	198:255	In this study, a novel intelligent antimicrobial model was constructed based on the antibiotic properties of nano-silver and the ion-exchange response of dehydrated alginate (Alg) gel.
26159674	3	31	theme	distinguished	398:410	arg1	feature					412:418	The distinguished feature	394:418	The distinguished feature of this model	394:432	The distinguished feature of this model lies in its antimicrobial properties and biocompatibility.
26159674	4	32	theme	releasing	512:520	arg1	level					522:526	the releasing level	508:526	the releasing level of nano-silver	508:541	In this model, the releasing level of nano-silver is determined by the outside-in swelling of Alg composites, which is further self-regulated by the volume of wound exudates.
26159674	5	33	theme	released	696:703	arg1	nano-silver					705:715	the released nano-silver	692:715	the released nano-silver	692:715	The results showed that the released nano-silver was intelligently maintained within a constant concentration range, so that it could be further designed to exhibit antimicrobial activity without cytotoxicity.
26159674	7	34	theme	swelling	1031:1038	arg1	feature					1040:1046	The self-regulating swelling feature	1011:1046	The self-regulating swelling feature based on the ion-exchange response of Alg along with the controlled release of nano-silver	1011:1137	The self-regulating swelling feature based on the ion-exchange response of Alg along with the controlled release of nano-silver made this composite a promising intelligent model for antimicrobial wound dressing applications.
26159674	1	35	theme	response	215:222	arg1	properties					168:177	the antibiotic properties	153:177	the antibiotic properties of nano-silver and the ion-exchange response of dehydrated alginate (Alg) gel	153:255	In this study, a novel intelligent antimicrobial model was constructed based on the antibiotic properties of nano-silver and the ion-exchange response of dehydrated alginate (Alg) gel.
26159674	0	36	theme	ion-exchange	51:62	arg1	stimuli					64:70	the ion-exchange stimuli	47:70	the ion-exchange stimuli	47:70	A self-regulating antimicrobial model based on the ion-exchange stimuli.
26159674	6	37	theme	significant	970:980	arg1	decrease					982:989	a significant decrease	968:989	a significant decrease of bacteria number	968:1008	Furthermore, the murine wound infection model conducted with these composites resulted in a significant decrease of bacteria number.
26159674	1	38	theme	intelligent	96:106	arg1	model					122:126	a novel intelligent antimicrobial model	88:126	a novel intelligent antimicrobial model	88:126	In this study, a novel intelligent antimicrobial model was constructed based on the antibiotic properties of nano-silver and the ion-exchange response of dehydrated alginate (Alg) gel.
26159674	7	39	theme	a	1159:1159	arg1	model					1183:1187	this composite a promising intelligent model	1144:1187	this composite a promising intelligent model for antimicrobial wound dressing applications	1144:1233	The self-regulating swelling feature based on the ion-exchange response of Alg along with the controlled release of nano-silver made this composite a promising intelligent model for antimicrobial wound dressing applications.
26159674	7	40	theme	ion-exchange	1061:1072	arg1	response					1074:1081	the ion-exchange response	1057:1081	the ion-exchange response of Alg along with the controlled release of nano-silver	1057:1137	The self-regulating swelling feature based on the ion-exchange response of Alg along with the controlled release of nano-silver made this composite a promising intelligent model for antimicrobial wound dressing applications.
26159674	7	41	theme	release	1116:1122	arg1	response					1074:1081	the ion-exchange response	1057:1081	the ion-exchange response of Alg along with the controlled release of nano-silver	1057:1137	The self-regulating swelling feature based on the ion-exchange response of Alg along with the controlled release of nano-silver made this composite a promising intelligent model for antimicrobial wound dressing applications.
26159674	1	42	theme	antimicrobial	108:120	arg1	model					122:126	a novel intelligent antimicrobial model	88:126	a novel intelligent antimicrobial model	88:126	In this study, a novel intelligent antimicrobial model was constructed based on the antibiotic properties of nano-silver and the ion-exchange response of dehydrated alginate (Alg) gel.
26159674	7	43	theme	self-regulating	1015:1029	arg1	feature					1040:1046	The self-regulating swelling feature	1011:1046	The self-regulating swelling feature based on the ion-exchange response of Alg along with the controlled release of nano-silver	1011:1137	The self-regulating swelling feature based on the ion-exchange response of Alg along with the controlled release of nano-silver made this composite a promising intelligent model for antimicrobial wound dressing applications.
26159674	4	44	theme	composites	591:600	arg1	swelling					575:582	the outside-in swelling	560:582	the outside-in swelling	560:582	In this model, the releasing level of nano-silver is determined by the outside-in swelling of Alg composites, which is further self-regulated by the volume of wound exudates.
26159674	1	45	theme	dehydrated	227:236	arg1	gel					253:255	dehydrated alginate (Alg) gel	227:255	dehydrated alginate (Alg) gel	227:255	In this study, a novel intelligent antimicrobial model was constructed based on the antibiotic properties of nano-silver and the ion-exchange response of dehydrated alginate (Alg) gel.
26159674	5	46	theme	antimicrobial	833:845	arg1	activity					847:854	antimicrobial activity	833:854	antimicrobial activity	833:854	The results showed that the released nano-silver was intelligently maintained within a constant concentration range, so that it could be further designed to exhibit antimicrobial activity without cytotoxicity.
26159674	1	47	theme	alginate	238:245	arg1	gel					253:255	dehydrated alginate (Alg) gel	227:255	dehydrated alginate (Alg) gel	227:255	In this study, a novel intelligent antimicrobial model was constructed based on the antibiotic properties of nano-silver and the ion-exchange response of dehydrated alginate (Alg) gel.
26159674	6	48	theme	bacteria	994:1001	arg1	number					1003:1008	bacteria number	994:1008	bacteria number	994:1008	Furthermore, the murine wound infection model conducted with these composites resulted in a significant decrease of bacteria number.
26159674	4	49	theme	exudates	658:665	arg1	volume					642:647	the volume	638:647	the volume of wound exudates	638:665	In this model, the releasing level of nano-silver is determined by the outside-in swelling of Alg composites, which is further self-regulated by the volume of wound exudates.
25686994	3	0	from	microscopy	664:673	arg1	SBF					866:868	SBF	866:868	SBF	866:868	The structure and properties of these composite scaffolds were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), mechanical properties testing (compressive, bending, tensile and impact), thermogravimetry/differential thermal analysis (TG/DTA), and physical stability test in simulated body fluid (SBF).
25686994	3	0	from	microscopy	664:673	arg1	fluid					859:863	simulated body fluid	844:863	simulated body fluid (SBF)	844:869	The structure and properties of these composite scaffolds were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), mechanical properties testing (compressive, bending, tensile and impact), thermogravimetry/differential thermal analysis (TG/DTA), and physical stability test in simulated body fluid (SBF).
25686994	3	1	theme	mechanical	682:691	arg1	properties					693:702	mechanical properties	682:702	mechanical properties testing (compressive, bending, tensile and impact)	682:753	The structure and properties of these composite scaffolds were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), mechanical properties testing (compressive, bending, tensile and impact), thermogravimetry/differential thermal analysis (TG/DTA), and physical stability test in simulated body fluid (SBF).
25686994	1	2	theme	hybrid	160:165	arg1	composites					175:184	Biodegradable porous hybrid polymer composites	139:184	Biodegradable porous hybrid polymer composites	139:184	Biodegradable porous hybrid polymer composites were prepared by using gelatin as base polymer matrix, β-tricalcium phosphate (TCP) and calcium sulfate (CS) as cementing materials, chitosan as an antimicrobial agent, and glutaraldehyde and polyethylene glycol (PEG) as crosslinkers at different mass ratios.
25686994	6	3	theme	size	1311:1314	arg1	distribution					1316:1327	the smallest pore size distribution	1293:1327	the smallest pore size distribution	1293:1327	The chemically crosslinked composite with 40% TCP followed by γ-radiation sterilization showed the smallest pore size distribution with a mean pore diameter of 159.22μm, which falls in the range of 100-350μm - known to be suitable for osteoconduction.
25686994	5	4	theme	thermal	1178:1184	arg1	properties					1186:1195	improved mechanical and thermal properties	1154:1195	improved mechanical and thermal properties	1154:1195	γ-Radiation sterilization triggered further cross linking in polymer matrix resulting a decrease in pore size of the composites and an increase in pore wall thickness with improved mechanical and thermal properties.
25686994	7	5	theme	mechanical	1475:1484	arg1	properties					1498:1507	its improved mechanical and thermal properties	1462:1507	its improved mechanical and thermal properties	1462:1507	Considering its improved mechanical and thermal properties along with osteoconduction ability without cytotoxicity, we propose this biocomposite as a viable candidate for bone tissue engineering.
25686994	5	6	theme	polymer	1043:1049	arg1	matrix					1051:1056	polymer matrix	1043:1056	polymer matrix resulting a decrease in pore size of the composites and an increase in pore wall thickness with improved mechanical and thermal properties	1043:1195	γ-Radiation sterilization triggered further cross linking in polymer matrix resulting a decrease in pore size of the composites and an increase in pore wall thickness with improved mechanical and thermal properties.
25686994	1	7	theme	polymer	167:173	arg1	composites					175:184	Biodegradable porous hybrid polymer composites	139:184	Biodegradable porous hybrid polymer composites	139:184	Biodegradable porous hybrid polymer composites were prepared by using gelatin as base polymer matrix, β-tricalcium phosphate (TCP) and calcium sulfate (CS) as cementing materials, chitosan as an antimicrobial agent, and glutaraldehyde and polyethylene glycol (PEG) as crosslinkers at different mass ratios.
25686994	3	8	theme	simulated	844:852	arg1	SBF					866:868	SBF	866:868	SBF	866:868	The structure and properties of these composite scaffolds were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), mechanical properties testing (compressive, bending, tensile and impact), thermogravimetry/differential thermal analysis (TG/DTA), and physical stability test in simulated body fluid (SBF).
25686994	3	8	theme	simulated	844:852	arg1	fluid					859:863	simulated body fluid	844:863	simulated body fluid (SBF)	844:869	The structure and properties of these composite scaffolds were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), mechanical properties testing (compressive, bending, tensile and impact), thermogravimetry/differential thermal analysis (TG/DTA), and physical stability test in simulated body fluid (SBF).
25686994	0	9	theme	gamma	95:99	arg1	irradiation					101:111	gamma irradiation	95:111	gamma irradiation	95:111	Preparation of gelatin based porous biocomposite for bone tissue engineering and evaluation of gamma irradiation effect on its properties.
25686994	6	10	theme	γ-radiation	1260:1270	arg1	sterilization					1272:1284	γ-radiation sterilization	1260:1284	γ-radiation sterilization	1260:1284	The chemically crosslinked composite with 40% TCP followed by γ-radiation sterilization showed the smallest pore size distribution with a mean pore diameter of 159.22μm, which falls in the range of 100-350μm - known to be suitable for osteoconduction.
25686994	5	11	theme	improved	1154:1161	arg1	properties					1186:1195	improved mechanical and thermal properties	1154:1195	improved mechanical and thermal properties	1154:1195	γ-Radiation sterilization triggered further cross linking in polymer matrix resulting a decrease in pore size of the composites and an increase in pore wall thickness with improved mechanical and thermal properties.
25686994	3	12	theme	body	854:857	arg1	SBF					866:868	SBF	866:868	SBF	866:868	The structure and properties of these composite scaffolds were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), mechanical properties testing (compressive, bending, tensile and impact), thermogravimetry/differential thermal analysis (TG/DTA), and physical stability test in simulated body fluid (SBF).
25686994	3	12	theme	body	854:857	arg1	fluid					859:863	simulated body fluid	844:863	simulated body fluid (SBF)	844:869	The structure and properties of these composite scaffolds were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), mechanical properties testing (compressive, bending, tensile and impact), thermogravimetry/differential thermal analysis (TG/DTA), and physical stability test in simulated body fluid (SBF).
25686994	4	13	theme	enhanced	913:920	arg1	properties					933:942	enhanced mechanical properties	913:942	enhanced mechanical properties among all the crosslinked composites	913:979	We found that TCP rich composites showed enhanced mechanical properties among all the crosslinked composites.
25686994	2	14	theme	γ-radiation	491:501	arg1	sterilization					503:515	γ-radiation sterilization	491:515	γ-radiation sterilization	491:515	Thereafter, the composites were subjected to γ-radiation sterilization.
25686994	5	15	theme	pore	1082:1085	arg1	size					1087:1090	pore size	1082:1090	pore size of the composites	1082:1108	γ-Radiation sterilization triggered further cross linking in polymer matrix resulting a decrease in pore size of the composites and an increase in pore wall thickness with improved mechanical and thermal properties.
25686994	6	16	theme	159.22μm	1358:1365	arg1	diameter					1346:1353	a mean pore diameter	1334:1353	a mean pore diameter of 159.22μm, which falls in the range of 100-350μm - known to be suitable for osteoconduction	1334:1447	The chemically crosslinked composite with 40% TCP followed by γ-radiation sterilization showed the smallest pore size distribution with a mean pore diameter of 159.22μm, which falls in the range of 100-350μm - known to be suitable for osteoconduction.
25686994	7	17	theme	improved	1466:1473	arg1	properties					1498:1507	its improved mechanical and thermal properties	1462:1507	its improved mechanical and thermal properties	1462:1507	Considering its improved mechanical and thermal properties along with osteoconduction ability without cytotoxicity, we propose this biocomposite as a viable candidate for bone tissue engineering.
25686994	4	18	theme	crosslinked	958:968	arg1	composites					970:979	all the crosslinked composites	950:979	all the crosslinked composites	950:979	We found that TCP rich composites showed enhanced mechanical properties among all the crosslinked composites.
25686994	7	19	theme	thermal	1490:1496	arg1	properties					1498:1507	its improved mechanical and thermal properties	1462:1507	its improved mechanical and thermal properties	1462:1507	Considering its improved mechanical and thermal properties along with osteoconduction ability without cytotoxicity, we propose this biocomposite as a viable candidate for bone tissue engineering.
25686994	4	20	theme	mechanical	922:931	arg1	properties					933:942	enhanced mechanical properties	913:942	enhanced mechanical properties among all the crosslinked composites	913:979	We found that TCP rich composites showed enhanced mechanical properties among all the crosslinked composites.
25686994	1	21	theme	antimicrobial	334:346	arg1	agent					348:352	an antimicrobial agent	331:352	an antimicrobial agent	331:352	Biodegradable porous hybrid polymer composites were prepared by using gelatin as base polymer matrix, β-tricalcium phosphate (TCP) and calcium sulfate (CS) as cementing materials, chitosan as an antimicrobial agent, and glutaraldehyde and polyethylene glycol (PEG) as crosslinkers at different mass ratios.
25686994	6	22	theme	pore	1306:1309	arg1	size					1311:1314	the smallest pore size	1293:1314	the smallest pore size distribution	1293:1327	The chemically crosslinked composite with 40% TCP followed by γ-radiation sterilization showed the smallest pore size distribution with a mean pore diameter of 159.22μm, which falls in the range of 100-350μm - known to be suitable for osteoconduction.
25686994	1	23	theme	different	423:431	arg1	ratios					438:443	different mass ratios	423:443	different mass ratios	423:443	Biodegradable porous hybrid polymer composites were prepared by using gelatin as base polymer matrix, β-tricalcium phosphate (TCP) and calcium sulfate (CS) as cementing materials, chitosan as an antimicrobial agent, and glutaraldehyde and polyethylene glycol (PEG) as crosslinkers at different mass ratios.
25686994	7	24	theme	tissue	1626:1631	arg1	engineering					1633:1643	bone tissue engineering	1621:1643	bone tissue engineering	1621:1643	Considering its improved mechanical and thermal properties along with osteoconduction ability without cytotoxicity, we propose this biocomposite as a viable candidate for bone tissue engineering.
25686994	6	25	theme	40	1240:1241	arg1	%					1242:1242	%	1242:1242	%	1242:1242	The chemically crosslinked composite with 40% TCP followed by γ-radiation sterilization showed the smallest pore size distribution with a mean pore diameter of 159.22μm, which falls in the range of 100-350μm - known to be suitable for osteoconduction.
25686994	0	26	theme	irradiation	101:111	arg1	evaluation					81:90	evaluation	81:90	evaluation of gamma irradiation	81:111	Preparation of gelatin based porous biocomposite for bone tissue engineering and evaluation of gamma irradiation effect on its properties.
25686994	0	26	theme	irradiation	101:111	arg1	engineering					65:75	bone tissue engineering	53:75	bone tissue engineering	53:75	Preparation of gelatin based porous biocomposite for bone tissue engineering and evaluation of gamma irradiation effect on its properties.
25686994	3	27	theme	properties	693:702	arg1	testing					704:710	mechanical properties testing	682:710	mechanical properties testing (compressive, bending, tensile and impact)	682:753	The structure and properties of these composite scaffolds were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), mechanical properties testing (compressive, bending, tensile and impact), thermogravimetry/differential thermal analysis (TG/DTA), and physical stability test in simulated body fluid (SBF).
25686994	6	28	theme	pore	1341:1344	arg1	diameter					1346:1353	a mean pore diameter	1334:1353	a mean pore diameter of 159.22μm, which falls in the range of 100-350μm - known to be suitable for osteoconduction	1334:1447	The chemically crosslinked composite with 40% TCP followed by γ-radiation sterilization showed the smallest pore size distribution with a mean pore diameter of 159.22μm, which falls in the range of 100-350μm - known to be suitable for osteoconduction.
25686994	1	29	theme	mass	433:436	arg1	ratios					438:443	different mass ratios	423:443	different mass ratios	423:443	Biodegradable porous hybrid polymer composites were prepared by using gelatin as base polymer matrix, β-tricalcium phosphate (TCP) and calcium sulfate (CS) as cementing materials, chitosan as an antimicrobial agent, and glutaraldehyde and polyethylene glycol (PEG) as crosslinkers at different mass ratios.
25686994	7	30	theme	viable	1600:1605	arg1	biocomposite					1582:1593	this biocomposite	1577:1593	this biocomposite	1577:1593	Considering its improved mechanical and thermal properties along with osteoconduction ability without cytotoxicity, we propose this biocomposite as a viable candidate for bone tissue engineering.
25686994	7	30	theme	viable	1600:1605	arg1	candidate					1607:1615	a viable candidate	1598:1615	a viable candidate for bone tissue engineering	1598:1643	Considering its improved mechanical and thermal properties along with osteoconduction ability without cytotoxicity, we propose this biocomposite as a viable candidate for bone tissue engineering.
25686994	6	31	theme	%	1242:1242	arg1	TCP					1244:1246	40% TCP	1240:1246	40% TCP followed by γ-radiation sterilization	1240:1284	The chemically crosslinked composite with 40% TCP followed by γ-radiation sterilization showed the smallest pore size distribution with a mean pore diameter of 159.22μm, which falls in the range of 100-350μm - known to be suitable for osteoconduction.
25686994	3	32	from	spectroscopy	625:636	arg1	SBF					866:868	SBF	866:868	SBF	866:868	The structure and properties of these composite scaffolds were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), mechanical properties testing (compressive, bending, tensile and impact), thermogravimetry/differential thermal analysis (TG/DTA), and physical stability test in simulated body fluid (SBF).
25686994	3	32	from	spectroscopy	625:636	arg1	fluid					859:863	simulated body fluid	844:863	simulated body fluid (SBF)	844:869	The structure and properties of these composite scaffolds were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), mechanical properties testing (compressive, bending, tensile and impact), thermogravimetry/differential thermal analysis (TG/DTA), and physical stability test in simulated body fluid (SBF).
25686994	3	33	from	testing	704:710	arg1	SBF					866:868	SBF	866:868	SBF	866:868	The structure and properties of these composite scaffolds were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), mechanical properties testing (compressive, bending, tensile and impact), thermogravimetry/differential thermal analysis (TG/DTA), and physical stability test in simulated body fluid (SBF).
25686994	3	33	from	testing	704:710	arg1	fluid					859:863	simulated body fluid	844:863	simulated body fluid (SBF)	844:869	The structure and properties of these composite scaffolds were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), mechanical properties testing (compressive, bending, tensile and impact), thermogravimetry/differential thermal analysis (TG/DTA), and physical stability test in simulated body fluid (SBF).
25686994	6	34	link	crosslinked	1213:1223	arg1	composite					1225:1233	The chemically crosslinked composite	1198:1233	The chemically crosslinked composite with 40% TCP followed by γ-radiation sterilization	1198:1284	The chemically crosslinked composite with 40% TCP followed by γ-radiation sterilization showed the smallest pore size distribution with a mean pore diameter of 159.22μm, which falls in the range of 100-350μm - known to be suitable for osteoconduction.
25686994	4	35	link	crosslinked	958:968	arg1	composites					970:979	all the crosslinked composites	950:979	all the crosslinked composites	950:979	We found that TCP rich composites showed enhanced mechanical properties among all the crosslinked composites.
25686994	3	36	theme	composite	556:564	arg1	scaffolds					566:574	these composite scaffolds	550:574	these composite scaffolds	550:574	The structure and properties of these composite scaffolds were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), mechanical properties testing (compressive, bending, tensile and impact), thermogravimetry/differential thermal analysis (TG/DTA), and physical stability test in simulated body fluid (SBF).
25686994	1	37	theme	calcium	274:280	arg1	sulfate					282:288	calcium sulfate	274:288	calcium sulfate (CS)	274:293	Biodegradable porous hybrid polymer composites were prepared by using gelatin as base polymer matrix, β-tricalcium phosphate (TCP) and calcium sulfate (CS) as cementing materials, chitosan as an antimicrobial agent, and glutaraldehyde and polyethylene glycol (PEG) as crosslinkers at different mass ratios.
25686994	1	37	theme	calcium	274:280	arg1	CS					291:292	CS	291:292	CS	291:292	Biodegradable porous hybrid polymer composites were prepared by using gelatin as base polymer matrix, β-tricalcium phosphate (TCP) and calcium sulfate (CS) as cementing materials, chitosan as an antimicrobial agent, and glutaraldehyde and polyethylene glycol (PEG) as crosslinkers at different mass ratios.
25686994	6	38	theme	mean	1336:1339	arg1	diameter					1346:1353	a mean pore diameter	1334:1353	a mean pore diameter of 159.22μm, which falls in the range of 100-350μm - known to be suitable for osteoconduction	1334:1447	The chemically crosslinked composite with 40% TCP followed by γ-radiation sterilization showed the smallest pore size distribution with a mean pore diameter of 159.22μm, which falls in the range of 100-350μm - known to be suitable for osteoconduction.
25686994	0	39	dep	engineering	65:75	arg1	effect					113:118	effect	113:118	effect on its properties	113:136	Preparation of gelatin based porous biocomposite for bone tissue engineering and evaluation of gamma irradiation effect on its properties.
25686994	3	40	dep	Fourier	598:604	arg1	transform					606:614	transform	606:614	transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), mechanical properties testing (compressive, bending, tensile and impact), thermogravimetry/differential thermal analysis (TG/DTA), and physical stability test in simulated body fluid (SBF)	606:869	The structure and properties of these composite scaffolds were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), mechanical properties testing (compressive, bending, tensile and impact), thermogravimetry/differential thermal analysis (TG/DTA), and physical stability test in simulated body fluid (SBF).
25686994	0	41	theme	gelatin	15:21	arg1	Preparation					0:10	Preparation	0:10	Preparation of gelatin	0:21	Preparation of gelatin based porous biocomposite for bone tissue engineering and evaluation of gamma irradiation effect on its properties.
25686994	5	42	theme	mechanical	1163:1172	arg1	properties					1186:1195	improved mechanical and thermal properties	1154:1195	improved mechanical and thermal properties	1154:1195	γ-Radiation sterilization triggered further cross linking in polymer matrix resulting a decrease in pore size of the composites and an increase in pore wall thickness with improved mechanical and thermal properties.
25686994	5	43	from	increase	1117:1124	arg1	thickness					1139:1147	pore wall thickness	1129:1147	pore wall thickness with improved mechanical and thermal properties	1129:1195	γ-Radiation sterilization triggered further cross linking in polymer matrix resulting a decrease in pore size of the composites and an increase in pore wall thickness with improved mechanical and thermal properties.
25686994	5	43	from	increase	1117:1124	arg1	size					1087:1090	pore size	1082:1090	pore size of the composites	1082:1108	γ-Radiation sterilization triggered further cross linking in polymer matrix resulting a decrease in pore size of the composites and an increase in pore wall thickness with improved mechanical and thermal properties.
25686994	5	44	theme	further	1018:1024	arg1	cross					1026:1030	further cross linking	1018:1038	further cross linking	1018:1038	γ-Radiation sterilization triggered further cross linking in polymer matrix resulting a decrease in pore size of the composites and an increase in pore wall thickness with improved mechanical and thermal properties.
25686994	6	45	theme	100-350μm	1396:1404	arg1	range					1387:1391	the range	1383:1391	the range of 100-350μm - known to be suitable for osteoconduction	1383:1447	The chemically crosslinked composite with 40% TCP followed by γ-radiation sterilization showed the smallest pore size distribution with a mean pore diameter of 159.22μm, which falls in the range of 100-350μm - known to be suitable for osteoconduction.
25686994	5	46	theme	γ-Radiation	982:992	arg1	sterilization					994:1006	γ-Radiation sterilization	982:1006	γ-Radiation sterilization	982:1006	γ-Radiation sterilization triggered further cross linking in polymer matrix resulting a decrease in pore size of the composites and an increase in pore wall thickness with improved mechanical and thermal properties.
25686994	4	47	theme	rich	890:893	arg1	composites					895:904	TCP rich composites	886:904	TCP rich composites	886:904	We found that TCP rich composites showed enhanced mechanical properties among all the crosslinked composites.
25686994	5	48	theme	linking	1032:1038	arg1	cross					1026:1030	further cross linking	1018:1038	further cross linking	1018:1038	γ-Radiation sterilization triggered further cross linking in polymer matrix resulting a decrease in pore size of the composites and an increase in pore wall thickness with improved mechanical and thermal properties.
25686994	6	49	with	composite	1225:1233	arg1	TCP					1244:1246	40% TCP	1240:1246	40% TCP followed by γ-radiation sterilization	1240:1284	The chemically crosslinked composite with 40% TCP followed by γ-radiation sterilization showed the smallest pore size distribution with a mean pore diameter of 159.22μm, which falls in the range of 100-350μm - known to be suitable for osteoconduction.
25686994	0	50	theme	porous	29:34	arg1	biocomposite					36:47	porous biocomposite	29:47	porous biocomposite for bone tissue engineering and evaluation of gamma irradiation effect on its properties	29:136	Preparation of gelatin based porous biocomposite for bone tissue engineering and evaluation of gamma irradiation effect on its properties.
25686994	4	51	theme	TCP	886:888	arg1	composites					895:904	TCP rich composites	886:904	TCP rich composites	886:904	We found that TCP rich composites showed enhanced mechanical properties among all the crosslinked composites.
25686994	1	52	theme	base	220:223	arg1	matrix					233:238	base polymer matrix	220:238	base polymer matrix	220:238	Biodegradable porous hybrid polymer composites were prepared by using gelatin as base polymer matrix, β-tricalcium phosphate (TCP) and calcium sulfate (CS) as cementing materials, chitosan as an antimicrobial agent, and glutaraldehyde and polyethylene glycol (PEG) as crosslinkers at different mass ratios.
25686994	0	53	theme	bone	53:56	arg1	engineering					65:75	bone tissue engineering	53:75	bone tissue engineering	53:75	Preparation of gelatin based porous biocomposite for bone tissue engineering and evaluation of gamma irradiation effect on its properties.
25686994	3	54	dep	testing	704:710	arg1	impact					747:752	impact	747:752	impact	747:752	The structure and properties of these composite scaffolds were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), mechanical properties testing (compressive, bending, tensile and impact), thermogravimetry/differential thermal analysis (TG/DTA), and physical stability test in simulated body fluid (SBF).
25686994	3	54	dep	testing	704:710	arg1	tensile					735:741	tensile	735:741	tensile	735:741	The structure and properties of these composite scaffolds were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), mechanical properties testing (compressive, bending, tensile and impact), thermogravimetry/differential thermal analysis (TG/DTA), and physical stability test in simulated body fluid (SBF).
25686994	3	54	dep	testing	704:710	arg1	bending					726:732	bending	726:732	bending	726:732	The structure and properties of these composite scaffolds were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), mechanical properties testing (compressive, bending, tensile and impact), thermogravimetry/differential thermal analysis (TG/DTA), and physical stability test in simulated body fluid (SBF).
25686994	3	54	dep	testing	704:710	arg1	compressive					713:723	compressive	713:723	compressive	713:723	The structure and properties of these composite scaffolds were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), mechanical properties testing (compressive, bending, tensile and impact), thermogravimetry/differential thermal analysis (TG/DTA), and physical stability test in simulated body fluid (SBF).
25686994	5	55	from	decrease	1070:1077	arg1	thickness					1139:1147	pore wall thickness	1129:1147	pore wall thickness with improved mechanical and thermal properties	1129:1195	γ-Radiation sterilization triggered further cross linking in polymer matrix resulting a decrease in pore size of the composites and an increase in pore wall thickness with improved mechanical and thermal properties.
25686994	5	55	from	decrease	1070:1077	arg1	size					1087:1090	pore size	1082:1090	pore size of the composites	1082:1108	γ-Radiation sterilization triggered further cross linking in polymer matrix resulting a decrease in pore size of the composites and an increase in pore wall thickness with improved mechanical and thermal properties.
25686994	1	56	theme	polyethylene	378:389	arg1	PEG					399:401	PEG	399:401	PEG	399:401	Biodegradable porous hybrid polymer composites were prepared by using gelatin as base polymer matrix, β-tricalcium phosphate (TCP) and calcium sulfate (CS) as cementing materials, chitosan as an antimicrobial agent, and glutaraldehyde and polyethylene glycol (PEG) as crosslinkers at different mass ratios.
25686994	1	56	theme	polyethylene	378:389	arg1	glycol					391:396	polyethylene glycol	378:396	polyethylene glycol (PEG)	378:402	Biodegradable porous hybrid polymer composites were prepared by using gelatin as base polymer matrix, β-tricalcium phosphate (TCP) and calcium sulfate (CS) as cementing materials, chitosan as an antimicrobial agent, and glutaraldehyde and polyethylene glycol (PEG) as crosslinkers at different mass ratios.
25686994	3	57	theme	scaffolds	566:574	arg1	structure					522:530	The structure	518:530	The structure	518:530	The structure and properties of these composite scaffolds were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), mechanical properties testing (compressive, bending, tensile and impact), thermogravimetry/differential thermal analysis (TG/DTA), and physical stability test in simulated body fluid (SBF).
25686994	3	57	theme	scaffolds	566:574	arg1	properties					536:545	properties	536:545	properties	536:545	The structure and properties of these composite scaffolds were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), mechanical properties testing (compressive, bending, tensile and impact), thermogravimetry/differential thermal analysis (TG/DTA), and physical stability test in simulated body fluid (SBF).
25686994	6	58	theme	smallest	1297:1304	arg1	size					1311:1314	the smallest pore size	1293:1314	the smallest pore size distribution	1293:1327	The chemically crosslinked composite with 40% TCP followed by γ-radiation sterilization showed the smallest pore size distribution with a mean pore diameter of 159.22μm, which falls in the range of 100-350μm - known to be suitable for osteoconduction.
25686994	7	59	theme	osteoconduction	1520:1534	arg1	ability					1536:1542	osteoconduction ability	1520:1542	osteoconduction ability	1520:1542	Considering its improved mechanical and thermal properties along with osteoconduction ability without cytotoxicity, we propose this biocomposite as a viable candidate for bone tissue engineering.
25686994	5	60	theme	wall	1134:1137	arg1	thickness					1139:1147	pore wall thickness	1129:1147	pore wall thickness with improved mechanical and thermal properties	1129:1195	γ-Radiation sterilization triggered further cross linking in polymer matrix resulting a decrease in pore size of the composites and an increase in pore wall thickness with improved mechanical and thermal properties.
25686994	5	61	theme	composites	1099:1108	arg1	size					1087:1090	pore size	1082:1090	pore size of the composites	1082:1108	γ-Radiation sterilization triggered further cross linking in polymer matrix resulting a decrease in pore size of the composites and an increase in pore wall thickness with improved mechanical and thermal properties.
25686994	0	62	from	effect	113:118	arg1	properties					127:136	its properties	123:136	its properties	123:136	Preparation of gelatin based porous biocomposite for bone tissue engineering and evaluation of gamma irradiation effect on its properties.
25686994	1	63	theme	cementing	298:306	arg1	materials					308:316	cementing materials	298:316	cementing materials	298:316	Biodegradable porous hybrid polymer composites were prepared by using gelatin as base polymer matrix, β-tricalcium phosphate (TCP) and calcium sulfate (CS) as cementing materials, chitosan as an antimicrobial agent, and glutaraldehyde and polyethylene glycol (PEG) as crosslinkers at different mass ratios.
25686994	3	64	from	analysis	794:801	arg1	SBF					866:868	SBF	866:868	SBF	866:868	The structure and properties of these composite scaffolds were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), mechanical properties testing (compressive, bending, tensile and impact), thermogravimetry/differential thermal analysis (TG/DTA), and physical stability test in simulated body fluid (SBF).
25686994	3	64	from	analysis	794:801	arg1	fluid					859:863	simulated body fluid	844:863	simulated body fluid (SBF)	844:869	The structure and properties of these composite scaffolds were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), mechanical properties testing (compressive, bending, tensile and impact), thermogravimetry/differential thermal analysis (TG/DTA), and physical stability test in simulated body fluid (SBF).
25686994	3	65	theme	infrared	616:623	arg1	FTIR					639:642	FTIR	639:642	FTIR	639:642	The structure and properties of these composite scaffolds were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), mechanical properties testing (compressive, bending, tensile and impact), thermogravimetry/differential thermal analysis (TG/DTA), and physical stability test in simulated body fluid (SBF).
25686994	3	65	theme	infrared	616:623	arg1	spectroscopy					625:636	infrared spectroscopy	616:636	infrared spectroscopy (FTIR)	616:643	The structure and properties of these composite scaffolds were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), mechanical properties testing (compressive, bending, tensile and impact), thermogravimetry/differential thermal analysis (TG/DTA), and physical stability test in simulated body fluid (SBF).
25686994	5	66	with	thickness	1139:1147	arg1	properties					1186:1195	improved mechanical and thermal properties	1154:1195	improved mechanical and thermal properties	1154:1195	γ-Radiation sterilization triggered further cross linking in polymer matrix resulting a decrease in pore size of the composites and an increase in pore wall thickness with improved mechanical and thermal properties.
25686994	3	67	from	test	836:839	arg1	SBF					866:868	SBF	866:868	SBF	866:868	The structure and properties of these composite scaffolds were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), mechanical properties testing (compressive, bending, tensile and impact), thermogravimetry/differential thermal analysis (TG/DTA), and physical stability test in simulated body fluid (SBF).
25686994	3	67	from	test	836:839	arg1	fluid					859:863	simulated body fluid	844:863	simulated body fluid (SBF)	844:869	The structure and properties of these composite scaffolds were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), mechanical properties testing (compressive, bending, tensile and impact), thermogravimetry/differential thermal analysis (TG/DTA), and physical stability test in simulated body fluid (SBF).
25686994	1	68	theme	polymer	225:231	arg1	matrix					233:238	base polymer matrix	220:238	base polymer matrix	220:238	Biodegradable porous hybrid polymer composites were prepared by using gelatin as base polymer matrix, β-tricalcium phosphate (TCP) and calcium sulfate (CS) as cementing materials, chitosan as an antimicrobial agent, and glutaraldehyde and polyethylene glycol (PEG) as crosslinkers at different mass ratios.
25686994	3	69	theme	scanning	646:653	arg1	SEM					676:678	SEM	676:678	SEM	676:678	The structure and properties of these composite scaffolds were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), mechanical properties testing (compressive, bending, tensile and impact), thermogravimetry/differential thermal analysis (TG/DTA), and physical stability test in simulated body fluid (SBF).
25686994	3	69	theme	scanning	646:653	arg1	microscopy					664:673	scanning electron microscopy	646:673	scanning electron microscopy (SEM)	646:679	The structure and properties of these composite scaffolds were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), mechanical properties testing (compressive, bending, tensile and impact), thermogravimetry/differential thermal analysis (TG/DTA), and physical stability test in simulated body fluid (SBF).
25686994	7	70	theme	bone	1621:1624	arg1	engineering					1633:1643	bone tissue engineering	1621:1643	bone tissue engineering	1621:1643	Considering its improved mechanical and thermal properties along with osteoconduction ability without cytotoxicity, we propose this biocomposite as a viable candidate for bone tissue engineering.
25686994	1	71	from	ratios	438:443	arg1	gelatin					209:215	gelatin	209:215	gelatin	209:215	Biodegradable porous hybrid polymer composites were prepared by using gelatin as base polymer matrix, β-tricalcium phosphate (TCP) and calcium sulfate (CS) as cementing materials, chitosan as an antimicrobial agent, and glutaraldehyde and polyethylene glycol (PEG) as crosslinkers at different mass ratios.
25686994	1	71	from	ratios	438:443	arg1	crosslinkers					407:418	crosslinkers	407:418	crosslinkers at different mass ratios	407:443	Biodegradable porous hybrid polymer composites were prepared by using gelatin as base polymer matrix, β-tricalcium phosphate (TCP) and calcium sulfate (CS) as cementing materials, chitosan as an antimicrobial agent, and glutaraldehyde and polyethylene glycol (PEG) as crosslinkers at different mass ratios.
25686994	3	72	theme	physical	817:824	arg1	test					836:839	physical stability test	817:839	physical stability test	817:839	The structure and properties of these composite scaffolds were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), mechanical properties testing (compressive, bending, tensile and impact), thermogravimetry/differential thermal analysis (TG/DTA), and physical stability test in simulated body fluid (SBF).
25686994	3	73	theme	electron	655:662	arg1	SEM					676:678	SEM	676:678	SEM	676:678	The structure and properties of these composite scaffolds were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), mechanical properties testing (compressive, bending, tensile and impact), thermogravimetry/differential thermal analysis (TG/DTA), and physical stability test in simulated body fluid (SBF).
25686994	3	73	theme	electron	655:662	arg1	microscopy					664:673	scanning electron microscopy	646:673	scanning electron microscopy (SEM)	646:679	The structure and properties of these composite scaffolds were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), mechanical properties testing (compressive, bending, tensile and impact), thermogravimetry/differential thermal analysis (TG/DTA), and physical stability test in simulated body fluid (SBF).
25686994	6	74	theme	crosslinked	1213:1223	arg1	composite					1225:1233	The chemically crosslinked composite	1198:1233	The chemically crosslinked composite with 40% TCP followed by γ-radiation sterilization	1198:1284	The chemically crosslinked composite with 40% TCP followed by γ-radiation sterilization showed the smallest pore size distribution with a mean pore diameter of 159.22μm, which falls in the range of 100-350μm - known to be suitable for osteoconduction.
25686994	1	75	theme	Biodegradable	139:151	arg1	composites					175:184	Biodegradable porous hybrid polymer composites	139:184	Biodegradable porous hybrid polymer composites	139:184	Biodegradable porous hybrid polymer composites were prepared by using gelatin as base polymer matrix, β-tricalcium phosphate (TCP) and calcium sulfate (CS) as cementing materials, chitosan as an antimicrobial agent, and glutaraldehyde and polyethylene glycol (PEG) as crosslinkers at different mass ratios.
25686994	3	76	theme	stability	826:834	arg1	test					836:839	physical stability test	817:839	physical stability test	817:839	The structure and properties of these composite scaffolds were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), mechanical properties testing (compressive, bending, tensile and impact), thermogravimetry/differential thermal analysis (TG/DTA), and physical stability test in simulated body fluid (SBF).
25686994	3	77	theme	thermogravimetry/differential	756:784	arg1	analysis					794:801	thermogravimetry/differential thermal analysis	756:801	thermogravimetry/differential thermal analysis (TG/DTA)	756:810	The structure and properties of these composite scaffolds were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), mechanical properties testing (compressive, bending, tensile and impact), thermogravimetry/differential thermal analysis (TG/DTA), and physical stability test in simulated body fluid (SBF).
25686994	3	77	theme	thermogravimetry/differential	756:784	arg1	TG/DTA					804:809	TG/DTA	804:809	TG/DTA	804:809	The structure and properties of these composite scaffolds were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), mechanical properties testing (compressive, bending, tensile and impact), thermogravimetry/differential thermal analysis (TG/DTA), and physical stability test in simulated body fluid (SBF).
25686994	0	78	theme	tissue	58:63	arg1	engineering					65:75	bone tissue engineering	53:75	bone tissue engineering	53:75	Preparation of gelatin based porous biocomposite for bone tissue engineering and evaluation of gamma irradiation effect on its properties.
25686994	5	79	theme	pore	1129:1132	arg1	thickness					1139:1147	pore wall thickness	1129:1147	pore wall thickness with improved mechanical and thermal properties	1129:1195	γ-Radiation sterilization triggered further cross linking in polymer matrix resulting a decrease in pore size of the composites and an increase in pore wall thickness with improved mechanical and thermal properties.
25686994	3	80	theme	thermal	786:792	arg1	analysis					794:801	thermogravimetry/differential thermal analysis	756:801	thermogravimetry/differential thermal analysis (TG/DTA)	756:810	The structure and properties of these composite scaffolds were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), mechanical properties testing (compressive, bending, tensile and impact), thermogravimetry/differential thermal analysis (TG/DTA), and physical stability test in simulated body fluid (SBF).
25686994	3	80	theme	thermal	786:792	arg1	TG/DTA					804:809	TG/DTA	804:809	TG/DTA	804:809	The structure and properties of these composite scaffolds were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), mechanical properties testing (compressive, bending, tensile and impact), thermogravimetry/differential thermal analysis (TG/DTA), and physical stability test in simulated body fluid (SBF).
25686994	1	81	theme	porous	153:158	arg1	composites					175:184	Biodegradable porous hybrid polymer composites	139:184	Biodegradable porous hybrid polymer composites	139:184	Biodegradable porous hybrid polymer composites were prepared by using gelatin as base polymer matrix, β-tricalcium phosphate (TCP) and calcium sulfate (CS) as cementing materials, chitosan as an antimicrobial agent, and glutaraldehyde and polyethylene glycol (PEG) as crosslinkers at different mass ratios.
25686994	1	82	theme	β-tricalcium	241:252	arg1	TCP					265:267	TCP	265:267	TCP	265:267	Biodegradable porous hybrid polymer composites were prepared by using gelatin as base polymer matrix, β-tricalcium phosphate (TCP) and calcium sulfate (CS) as cementing materials, chitosan as an antimicrobial agent, and glutaraldehyde and polyethylene glycol (PEG) as crosslinkers at different mass ratios.
25686994	1	82	theme	β-tricalcium	241:252	arg1	phosphate					254:262	β-tricalcium phosphate	241:262	β-tricalcium phosphate (TCP)	241:268	Biodegradable porous hybrid polymer composites were prepared by using gelatin as base polymer matrix, β-tricalcium phosphate (TCP) and calcium sulfate (CS) as cementing materials, chitosan as an antimicrobial agent, and glutaraldehyde and polyethylene glycol (PEG) as crosslinkers at different mass ratios.
25701653	4	0	theme	types	599:603	arg1	enoxaparin					615:624	enoxaparin	615:624	enoxaparin	615:624	The chain fragments of two types of LMWHs, enoxaparin and nadroparin, were generated by controlled enzymatic digestion with each of heparinase I (Hep I, Enzyme Commission (EC) # 4.2.2.7), heparinase II (Hep II, no EC # assigned) and heparinase III (Hep III, EC # 4.2.2.8).
25701653	4	0	theme	types	599:603	arg1	fragments					582:590	The chain fragments	572:590	The chain fragments of two types of LMWHs	572:612	The chain fragments of two types of LMWHs, enoxaparin and nadroparin, were generated by controlled enzymatic digestion with each of heparinase I (Hep I, Enzyme Commission (EC) # 4.2.2.7), heparinase II (Hep II, no EC # assigned) and heparinase III (Hep III, EC # 4.2.2.8).
25701653	4	0	theme	types	599:603	arg1	nadroparin					630:639	nadroparin	630:639	nadroparin	630:639	The chain fragments of two types of LMWHs, enoxaparin and nadroparin, were generated by controlled enzymatic digestion with each of heparinase I (Hep I, Enzyme Commission (EC) # 4.2.2.7), heparinase II (Hep II, no EC # assigned) and heparinase III (Hep III, EC # 4.2.2.8).
25701653	11	1	dep	resolution	1879:1888	arg1	high					1874:1877	high	1874:1877	high	1874:1877	This approach to profile LMWHs' fragments offers a highly reproducible, high resolution and information-rich tool for evaluating the quality of this category of anticoagulant drugs or comparing structural similarities among samples from various sources.
25701653	6	2	theme	theoretical	1124:1134	arg1	compositions					1147:1158	all theoretical structural compositions	1120:1158	all theoretical structural compositions	1120:1158	A database containing all theoretical structural compositions was established to assist the mass spectra interpretation.
25701653	4	3	theme	LMWHs	608:612	arg1	types					599:603	two types	595:603	two types of LMWHs	595:612	The chain fragments of two types of LMWHs, enoxaparin and nadroparin, were generated by controlled enzymatic digestion with each of heparinase I (Hep I, Enzyme Commission (EC) # 4.2.2.7), heparinase II (Hep II, no EC # assigned) and heparinase III (Hep III, EC # 4.2.2.8).
25701653	2	4	theme	unfractionated	301:314	arg1	UFH					325:327	UFH	325:327	UFH	325:327	Compared to unfractionated heparin (UFH), LMWHs are prevalently used as clinical anticoagulant drugs due to their lower side effects and better bioavailability.
25701653	2	4	theme	unfractionated	301:314	arg1	heparin					316:322	unfractionated heparin	301:322	unfractionated heparin (UFH)	301:328	Compared to unfractionated heparin (UFH), LMWHs are prevalently used as clinical anticoagulant drugs due to their lower side effects and better bioavailability.
25701653	4	5	theme	enzymatic	671:679	arg1	digestion					681:689	controlled enzymatic digestion	660:689	controlled enzymatic digestion with each of heparinase I (Hep I, Enzyme Commission (EC) # 4.2.2.7), heparinase II (Hep II, no EC # assigned) and heparinase III (Hep III, EC # 4.2.2.8)	660:842	The chain fragments of two types of LMWHs, enoxaparin and nadroparin, were generated by controlled enzymatic digestion with each of heparinase I (Hep I, Enzyme Commission (EC) # 4.2.2.7), heparinase II (Hep II, no EC # assigned) and heparinase III (Hep III, EC # 4.2.2.8).
25701653	1	6	theme	linear	178:183	arg1	polymers					217:224	linear and highly charged carbohydrate polymers	178:224	linear and highly charged carbohydrate polymers prepared by chemical or enzymatic depolymerization of heparin	178:286	Low molecular weight heparins (LMWHs) are linear and highly charged carbohydrate polymers prepared by chemical or enzymatic depolymerization of heparin.
25701653	1	6	theme	linear	178:183	arg1	heparins					157:164	Low molecular weight heparins	136:164	Low molecular weight heparins (LMWHs)	136:172	Low molecular weight heparins (LMWHs) are linear and highly charged carbohydrate polymers prepared by chemical or enzymatic depolymerization of heparin.
25701653	6	7	theme	spectra	1195:1201	arg1	interpretation					1203:1216	the mass spectra interpretation	1186:1216	the mass spectra interpretation	1186:1216	A database containing all theoretical structural compositions was established to assist the mass spectra interpretation.
25701653	1	8	theme	carbohydrate	204:215	arg1	polymers					217:224	linear and highly charged carbohydrate polymers	178:224	linear and highly charged carbohydrate polymers prepared by chemical or enzymatic depolymerization of heparin	178:286	Low molecular weight heparins (LMWHs) are linear and highly charged carbohydrate polymers prepared by chemical or enzymatic depolymerization of heparin.
25701653	1	8	theme	carbohydrate	204:215	arg1	heparins					157:164	Low molecular weight heparins	136:164	Low molecular weight heparins (LMWHs)	136:172	Low molecular weight heparins (LMWHs) are linear and highly charged carbohydrate polymers prepared by chemical or enzymatic depolymerization of heparin.
25701653	6	9	theme	mass	1190:1193	arg1	spectra					1195:1201	the mass spectra	1186:1201	the mass spectra interpretation	1186:1216	A database containing all theoretical structural compositions was established to assist the mass spectra interpretation.
25701653	5	10	used	used	1008:1011	arg2	chromatography					893:906	Reversed phase ion pair high performance liquid chromatography	845:906	Reversed phase ion pair high performance liquid chromatography (RPIP-HPLC) coupled with electrospray ion trap time-of-flight mass spectrometry (ESI-IT-TOF-MS)	845:1002	Reversed phase ion pair high performance liquid chromatography (RPIP-HPLC) coupled with electrospray ion trap time-of-flight mass spectrometry (ESI-IT-TOF-MS) was used to profile the oligosaccharide chains ranging from disaccharides to decasaccharides.
25701653	5	10	used	used	1008:1011	arg2	RPIP-HPLC					909:917	RPIP-HPLC	909:917	RPIP-HPLC	909:917	Reversed phase ion pair high performance liquid chromatography (RPIP-HPLC) coupled with electrospray ion trap time-of-flight mass spectrometry (ESI-IT-TOF-MS) was used to profile the oligosaccharide chains ranging from disaccharides to decasaccharides.
25701653	9	11	theme	LMWH	1452:1455	arg1	oligosaccharides					1457:1472	the common LMWH oligosaccharides	1441:1472	the common LMWH oligosaccharides	1441:1472	Besides the common LMWH oligosaccharides, many components containing characteristic LMWH structures such as saturated L-idopyranosuronic acid, 2,5-anhydro-D-mannitol, 1,6-anhydro-D-aminopyranose, as well as odd number oligosaccharides were also revealed.
25701653	10	12	theme	innovator	1745:1753	arg1	products					1778:1785	innovator and generic nadroparin products	1745:1785	innovator and generic nadroparin products	1745:1785	Quantitative comparison of major components derived from innovator and generic nadroparin products was presented.
25701653	4	13	theme	Hep	718:720	arg1	#					748:748	Enzyme Commission (EC) # 4.2.2.7	725:756	Enzyme Commission (EC) # 4.2.2.7	725:756	The chain fragments of two types of LMWHs, enoxaparin and nadroparin, were generated by controlled enzymatic digestion with each of heparinase I (Hep I, Enzyme Commission (EC) # 4.2.2.7), heparinase II (Hep II, no EC # assigned) and heparinase III (Hep III, EC # 4.2.2.8).
25701653	4	13	theme	Hep	718:720	arg1	I					722:722	Hep I	718:722	Hep I	718:722	The chain fragments of two types of LMWHs, enoxaparin and nadroparin, were generated by controlled enzymatic digestion with each of heparinase I (Hep I, Enzyme Commission (EC) # 4.2.2.7), heparinase II (Hep II, no EC # assigned) and heparinase III (Hep III, EC # 4.2.2.8).
25701653	5	14	theme	time-of-flight	955:968	arg1	spectrometry					975:986	electrospray ion trap time-of-flight mass spectrometry	933:986	electrospray ion trap time-of-flight mass spectrometry (ESI-IT-TOF-MS)	933:1002	Reversed phase ion pair high performance liquid chromatography (RPIP-HPLC) coupled with electrospray ion trap time-of-flight mass spectrometry (ESI-IT-TOF-MS) was used to profile the oligosaccharide chains ranging from disaccharides to decasaccharides.
25701653	5	14	theme	time-of-flight	955:968	arg1	ESI-IT-TOF-MS					989:1001	ESI-IT-TOF-MS	989:1001	ESI-IT-TOF-MS	989:1001	Reversed phase ion pair high performance liquid chromatography (RPIP-HPLC) coupled with electrospray ion trap time-of-flight mass spectrometry (ESI-IT-TOF-MS) was used to profile the oligosaccharide chains ranging from disaccharides to decasaccharides.
25701653	1	15	theme	Low	136:138	arg1	polymers					217:224	linear and highly charged carbohydrate polymers	178:224	linear and highly charged carbohydrate polymers prepared by chemical or enzymatic depolymerization of heparin	178:286	Low molecular weight heparins (LMWHs) are linear and highly charged carbohydrate polymers prepared by chemical or enzymatic depolymerization of heparin.
25701653	1	15	theme	Low	136:138	arg1	LMWHs					167:171	LMWHs	167:171	LMWHs	167:171	Low molecular weight heparins (LMWHs) are linear and highly charged carbohydrate polymers prepared by chemical or enzymatic depolymerization of heparin.
25701653	1	15	theme	Low	136:138	arg1	heparins					157:164	Low molecular weight heparins	136:164	Low molecular weight heparins (LMWHs)	136:172	Low molecular weight heparins (LMWHs) are linear and highly charged carbohydrate polymers prepared by chemical or enzymatic depolymerization of heparin.
25701653	1	16	theme	weight	150:155	arg1	polymers					217:224	linear and highly charged carbohydrate polymers	178:224	linear and highly charged carbohydrate polymers prepared by chemical or enzymatic depolymerization of heparin	178:286	Low molecular weight heparins (LMWHs) are linear and highly charged carbohydrate polymers prepared by chemical or enzymatic depolymerization of heparin.
25701653	1	16	theme	weight	150:155	arg1	LMWHs					167:171	LMWHs	167:171	LMWHs	167:171	Low molecular weight heparins (LMWHs) are linear and highly charged carbohydrate polymers prepared by chemical or enzymatic depolymerization of heparin.
25701653	1	16	theme	weight	150:155	arg1	heparins					157:164	Low molecular weight heparins	136:164	Low molecular weight heparins (LMWHs)	136:172	Low molecular weight heparins (LMWHs) are linear and highly charged carbohydrate polymers prepared by chemical or enzymatic depolymerization of heparin.
25701653	9	17	theme	many	1475:1478	arg1	components					1480:1489	many components	1475:1489	many components containing characteristic LMWH structures such as saturated L-idopyranosuronic acid, 2,5-anhydro-D-mannitol, 1,6-anhydro-D-aminopyranose, as well as odd number oligosaccharides	1475:1666	Besides the common LMWH oligosaccharides, many components containing characteristic LMWH structures such as saturated L-idopyranosuronic acid, 2,5-anhydro-D-mannitol, 1,6-anhydro-D-aminopyranose, as well as odd number oligosaccharides were also revealed.
25701653	7	18	theme	distinguishable	1294:1308	arg1	patterns					1325:1332	distinguishable fingerprinting patterns	1294:1332	distinguishable fingerprinting patterns	1294:1332	The six digests derived by three enzymes from two types of LMWHs exhibited distinguishable fingerprinting patterns.
25701653	10	19	theme	major	1715:1719	arg1	components					1721:1730	major components	1715:1730	major components derived from innovator and generic nadroparin products	1715:1785	Quantitative comparison of major components derived from innovator and generic nadroparin products was presented.
25701653	0	20	theme	reversed	58:65	arg1	spectrometry					122:133	reversed phase ion pair liquid chromatography-electrospray mass spectrometry	58:133	reversed phase ion pair liquid chromatography-electrospray mass spectrometry	58:133	Fragment profiling of low molecular weight heparins using reversed phase ion pair liquid chromatography-electrospray mass spectrometry.
25701653	11	21	theme	various	2039:2045	arg1	sources					2047:2053	various sources	2039:2053	various sources	2039:2053	This approach to profile LMWHs' fragments offers a highly reproducible, high resolution and information-rich tool for evaluating the quality of this category of anticoagulant drugs or comparing structural similarities among samples from various sources.
25701653	9	22	theme	characteristic	1502:1515	arg1	acid					1570:1573	saturated L-idopyranosuronic acid	1541:1573	saturated L-idopyranosuronic acid	1541:1573	Besides the common LMWH oligosaccharides, many components containing characteristic LMWH structures such as saturated L-idopyranosuronic acid, 2,5-anhydro-D-mannitol, 1,6-anhydro-D-aminopyranose, as well as odd number oligosaccharides were also revealed.
25701653	9	22	theme	characteristic	1502:1515	arg1	structures					1522:1531	characteristic LMWH structures	1502:1531	characteristic LMWH structures such as saturated L-idopyranosuronic acid, 2,5-anhydro-D-mannitol, 1,6-anhydro-D-aminopyranose,	1502:1627	Besides the common LMWH oligosaccharides, many components containing characteristic LMWH structures such as saturated L-idopyranosuronic acid, 2,5-anhydro-D-mannitol, 1,6-anhydro-D-aminopyranose, as well as odd number oligosaccharides were also revealed.
25701653	11	23	theme	profile	1819:1825	arg1	fragments					1834:1842	profile LMWHs' fragments	1819:1842	profile LMWHs' fragments	1819:1842	This approach to profile LMWHs' fragments offers a highly reproducible, high resolution and information-rich tool for evaluating the quality of this category of anticoagulant drugs or comparing structural similarities among samples from various sources.
25701653	0	24	theme	ion	73:75	arg1	spectrometry					122:133	reversed phase ion pair liquid chromatography-electrospray mass spectrometry	58:133	reversed phase ion pair liquid chromatography-electrospray mass spectrometry	58:133	Fragment profiling of low molecular weight heparins using reversed phase ion pair liquid chromatography-electrospray mass spectrometry.
25701653	0	25	theme	liquid	82:87	arg1	spectrometry					122:133	reversed phase ion pair liquid chromatography-electrospray mass spectrometry	58:133	reversed phase ion pair liquid chromatography-electrospray mass spectrometry	58:133	Fragment profiling of low molecular weight heparins using reversed phase ion pair liquid chromatography-electrospray mass spectrometry.
25701653	5	26	theme	performance	874:884	arg1	RPIP-HPLC					909:917	RPIP-HPLC	909:917	RPIP-HPLC	909:917	Reversed phase ion pair high performance liquid chromatography (RPIP-HPLC) coupled with electrospray ion trap time-of-flight mass spectrometry (ESI-IT-TOF-MS) was used to profile the oligosaccharide chains ranging from disaccharides to decasaccharides.
25701653	5	26	theme	performance	874:884	arg1	chromatography					893:906	Reversed phase ion pair high performance liquid chromatography	845:906	Reversed phase ion pair high performance liquid chromatography (RPIP-HPLC) coupled with electrospray ion trap time-of-flight mass spectrometry (ESI-IT-TOF-MS)	845:1002	Reversed phase ion pair high performance liquid chromatography (RPIP-HPLC) coupled with electrospray ion trap time-of-flight mass spectrometry (ESI-IT-TOF-MS) was used to profile the oligosaccharide chains ranging from disaccharides to decasaccharides.
25701653	2	27	theme	lower	403:407	arg1	effects					414:420	their lower side effects	397:420	their lower side effects	397:420	Compared to unfractionated heparin (UFH), LMWHs are prevalently used as clinical anticoagulant drugs due to their lower side effects and better bioavailability.
25701653	0	28	theme	mass	117:120	arg1	spectrometry					122:133	reversed phase ion pair liquid chromatography-electrospray mass spectrometry	58:133	reversed phase ion pair liquid chromatography-electrospray mass spectrometry	58:133	Fragment profiling of low molecular weight heparins using reversed phase ion pair liquid chromatography-electrospray mass spectrometry.
25701653	9	29	theme	L-idopyranosuronic	1551:1568	arg1	1,6-anhydro-D-aminopyranose					1600:1626	1,6-anhydro-D-aminopyranose	1600:1626	1,6-anhydro-D-aminopyranose	1600:1626	Besides the common LMWH oligosaccharides, many components containing characteristic LMWH structures such as saturated L-idopyranosuronic acid, 2,5-anhydro-D-mannitol, 1,6-anhydro-D-aminopyranose, as well as odd number oligosaccharides were also revealed.
25701653	9	29	theme	L-idopyranosuronic	1551:1568	arg1	2,5-anhydro-D-mannitol					1576:1597	2,5-anhydro-D-mannitol	1576:1597	2,5-anhydro-D-mannitol	1576:1597	Besides the common LMWH oligosaccharides, many components containing characteristic LMWH structures such as saturated L-idopyranosuronic acid, 2,5-anhydro-D-mannitol, 1,6-anhydro-D-aminopyranose, as well as odd number oligosaccharides were also revealed.
25701653	9	29	theme	L-idopyranosuronic	1551:1568	arg1	acid					1570:1573	saturated L-idopyranosuronic acid	1541:1573	saturated L-idopyranosuronic acid	1541:1573	Besides the common LMWH oligosaccharides, many components containing characteristic LMWH structures such as saturated L-idopyranosuronic acid, 2,5-anhydro-D-mannitol, 1,6-anhydro-D-aminopyranose, as well as odd number oligosaccharides were also revealed.
25701653	4	30	theme	Commission	732:741	arg1	#					748:748	Enzyme Commission (EC) # 4.2.2.7	725:756	Enzyme Commission (EC) # 4.2.2.7	725:756	The chain fragments of two types of LMWHs, enoxaparin and nadroparin, were generated by controlled enzymatic digestion with each of heparinase I (Hep I, Enzyme Commission (EC) # 4.2.2.7), heparinase II (Hep II, no EC # assigned) and heparinase III (Hep III, EC # 4.2.2.8).
25701653	4	30	theme	Commission	732:741	arg1	I					722:722	Hep I	718:722	Hep I	718:722	The chain fragments of two types of LMWHs, enoxaparin and nadroparin, were generated by controlled enzymatic digestion with each of heparinase I (Hep I, Enzyme Commission (EC) # 4.2.2.7), heparinase II (Hep II, no EC # assigned) and heparinase III (Hep III, EC # 4.2.2.8).
25701653	0	31	theme	Fragment	0:7	arg1	profiling					9:17	Fragment profiling	0:17	Fragment profiling of low molecular weight heparins	0:50	Fragment profiling of low molecular weight heparins using reversed phase ion pair liquid chromatography-electrospray mass spectrometry.
25701653	5	32	theme	phase	854:858	arg1	RPIP-HPLC					909:917	RPIP-HPLC	909:917	RPIP-HPLC	909:917	Reversed phase ion pair high performance liquid chromatography (RPIP-HPLC) coupled with electrospray ion trap time-of-flight mass spectrometry (ESI-IT-TOF-MS) was used to profile the oligosaccharide chains ranging from disaccharides to decasaccharides.
25701653	5	32	theme	phase	854:858	arg1	chromatography					893:906	Reversed phase ion pair high performance liquid chromatography	845:906	Reversed phase ion pair high performance liquid chromatography (RPIP-HPLC) coupled with electrospray ion trap time-of-flight mass spectrometry (ESI-IT-TOF-MS)	845:1002	Reversed phase ion pair high performance liquid chromatography (RPIP-HPLC) coupled with electrospray ion trap time-of-flight mass spectrometry (ESI-IT-TOF-MS) was used to profile the oligosaccharide chains ranging from disaccharides to decasaccharides.
25701653	2	33	used	used	353:356	arg2	drugs					384:388	clinical anticoagulant drugs	361:388	clinical anticoagulant drugs due to their lower side effects and better bioavailability	361:447	Compared to unfractionated heparin (UFH), LMWHs are prevalently used as clinical anticoagulant drugs due to their lower side effects and better bioavailability.
25701653	2	33	used	used	353:356	arg2	LMWHs					331:335	LMWHs	331:335	LMWHs	331:335	Compared to unfractionated heparin (UFH), LMWHs are prevalently used as clinical anticoagulant drugs due to their lower side effects and better bioavailability.
25701653	4	34	theme	EC	744:745	arg1	#					748:748	Enzyme Commission (EC) # 4.2.2.7	725:756	Enzyme Commission (EC) # 4.2.2.7	725:756	The chain fragments of two types of LMWHs, enoxaparin and nadroparin, were generated by controlled enzymatic digestion with each of heparinase I (Hep I, Enzyme Commission (EC) # 4.2.2.7), heparinase II (Hep II, no EC # assigned) and heparinase III (Hep III, EC # 4.2.2.8).
25701653	4	34	theme	EC	744:745	arg1	I					722:722	Hep I	718:722	Hep I	718:722	The chain fragments of two types of LMWHs, enoxaparin and nadroparin, were generated by controlled enzymatic digestion with each of heparinase I (Hep I, Enzyme Commission (EC) # 4.2.2.7), heparinase II (Hep II, no EC # assigned) and heparinase III (Hep III, EC # 4.2.2.8).
25701653	2	35	theme	better	426:431	arg1	bioavailability					433:447	better bioavailability	426:447	better bioavailability	426:447	Compared to unfractionated heparin (UFH), LMWHs are prevalently used as clinical anticoagulant drugs due to their lower side effects and better bioavailability.
25701653	5	36	theme	pair	864:867	arg1	RPIP-HPLC					909:917	RPIP-HPLC	909:917	RPIP-HPLC	909:917	Reversed phase ion pair high performance liquid chromatography (RPIP-HPLC) coupled with electrospray ion trap time-of-flight mass spectrometry (ESI-IT-TOF-MS) was used to profile the oligosaccharide chains ranging from disaccharides to decasaccharides.
25701653	5	36	theme	pair	864:867	arg1	chromatography					893:906	Reversed phase ion pair high performance liquid chromatography	845:906	Reversed phase ion pair high performance liquid chromatography (RPIP-HPLC) coupled with electrospray ion trap time-of-flight mass spectrometry (ESI-IT-TOF-MS)	845:1002	Reversed phase ion pair high performance liquid chromatography (RPIP-HPLC) coupled with electrospray ion trap time-of-flight mass spectrometry (ESI-IT-TOF-MS) was used to profile the oligosaccharide chains ranging from disaccharides to decasaccharides.
25701653	4	37	with	digestion	681:689	arg1	heparinase					805:814	heparinase III	805:818	heparinase III (Hep III, EC # 4.2.2.8)	805:842	The chain fragments of two types of LMWHs, enoxaparin and nadroparin, were generated by controlled enzymatic digestion with each of heparinase I (Hep I, Enzyme Commission (EC) # 4.2.2.7), heparinase II (Hep II, no EC # assigned) and heparinase III (Hep III, EC # 4.2.2.8).
25701653	4	37	with	digestion	681:689	arg1	each					696:699	each	696:699	each	696:699	The chain fragments of two types of LMWHs, enoxaparin and nadroparin, were generated by controlled enzymatic digestion with each of heparinase I (Hep I, Enzyme Commission (EC) # 4.2.2.7), heparinase II (Hep II, no EC # assigned) and heparinase III (Hep III, EC # 4.2.2.8).
25701653	4	37	with	digestion	681:689	arg1	I					715:715	heparinase I	704:715	heparinase I (Hep I, Enzyme Commission (EC) # 4.2.2.7)	704:757	The chain fragments of two types of LMWHs, enoxaparin and nadroparin, were generated by controlled enzymatic digestion with each of heparinase I (Hep I, Enzyme Commission (EC) # 4.2.2.7), heparinase II (Hep II, no EC # assigned) and heparinase III (Hep III, EC # 4.2.2.8).
25701653	4	37	with	digestion	681:689	arg1	heparinase					760:769	heparinase II	760:772	heparinase II (Hep II, no EC # assigned)	760:799	The chain fragments of two types of LMWHs, enoxaparin and nadroparin, were generated by controlled enzymatic digestion with each of heparinase I (Hep I, Enzyme Commission (EC) # 4.2.2.7), heparinase II (Hep II, no EC # assigned) and heparinase III (Hep III, EC # 4.2.2.8).
25701653	4	37	with	digestion	681:689	arg1	Hep					821:823	Hep	821:823	Hep	821:823	The chain fragments of two types of LMWHs, enoxaparin and nadroparin, were generated by controlled enzymatic digestion with each of heparinase I (Hep I, Enzyme Commission (EC) # 4.2.2.7), heparinase II (Hep II, no EC # assigned) and heparinase III (Hep III, EC # 4.2.2.8).
25701653	8	38	theme	enoxaparin	1353:1362	arg1	fragments					1364:1372	94 enoxaparin fragments	1350:1372	94 enoxaparin fragments	1350:1372	And a total of 94 enoxaparin fragments and 109 nadroparin fragments were detected and identified.
25701653	11	39	theme	anticoagulant	1963:1975	arg1	drugs					1977:1981	anticoagulant drugs	1963:1981	anticoagulant drugs	1963:1981	This approach to profile LMWHs' fragments offers a highly reproducible, high resolution and information-rich tool for evaluating the quality of this category of anticoagulant drugs or comparing structural similarities among samples from various sources.
25701653	0	40	theme	molecular	26:34	arg1	heparins					43:50	low molecular weight heparins	22:50	low molecular weight heparins	22:50	Fragment profiling of low molecular weight heparins using reversed phase ion pair liquid chromatography-electrospray mass spectrometry.
25701653	5	41	theme	electrospray	933:944	arg1	spectrometry					975:986	electrospray ion trap time-of-flight mass spectrometry	933:986	electrospray ion trap time-of-flight mass spectrometry (ESI-IT-TOF-MS)	933:1002	Reversed phase ion pair high performance liquid chromatography (RPIP-HPLC) coupled with electrospray ion trap time-of-flight mass spectrometry (ESI-IT-TOF-MS) was used to profile the oligosaccharide chains ranging from disaccharides to decasaccharides.
25701653	5	41	theme	electrospray	933:944	arg1	ESI-IT-TOF-MS					989:1001	ESI-IT-TOF-MS	989:1001	ESI-IT-TOF-MS	989:1001	Reversed phase ion pair high performance liquid chromatography (RPIP-HPLC) coupled with electrospray ion trap time-of-flight mass spectrometry (ESI-IT-TOF-MS) was used to profile the oligosaccharide chains ranging from disaccharides to decasaccharides.
25701653	3	42	theme	powerful	497:504	arg1	method					523:528	a rapid and powerful fragment mapping method	485:528	a rapid and powerful fragment mapping method for structural characterization of LMWHs	485:569	The work presented herein provides a rapid and powerful fragment mapping method for structural characterization of LMWHs.
25701653	0	43	theme	heparins	43:50	arg1	profiling					9:17	Fragment profiling	0:17	Fragment profiling of low molecular weight heparins	0:50	Fragment profiling of low molecular weight heparins using reversed phase ion pair liquid chromatography-electrospray mass spectrometry.
25701653	5	44	theme	trap	950:953	arg1	spectrometry					975:986	electrospray ion trap time-of-flight mass spectrometry	933:986	electrospray ion trap time-of-flight mass spectrometry (ESI-IT-TOF-MS)	933:1002	Reversed phase ion pair high performance liquid chromatography (RPIP-HPLC) coupled with electrospray ion trap time-of-flight mass spectrometry (ESI-IT-TOF-MS) was used to profile the oligosaccharide chains ranging from disaccharides to decasaccharides.
25701653	5	44	theme	trap	950:953	arg1	ESI-IT-TOF-MS					989:1001	ESI-IT-TOF-MS	989:1001	ESI-IT-TOF-MS	989:1001	Reversed phase ion pair high performance liquid chromatography (RPIP-HPLC) coupled with electrospray ion trap time-of-flight mass spectrometry (ESI-IT-TOF-MS) was used to profile the oligosaccharide chains ranging from disaccharides to decasaccharides.
25701653	7	45	theme	fingerprinting	1310:1323	arg1	patterns					1325:1332	distinguishable fingerprinting patterns	1294:1332	distinguishable fingerprinting patterns	1294:1332	The six digests derived by three enzymes from two types of LMWHs exhibited distinguishable fingerprinting patterns.
25701653	8	46	theme	nadroparin	1382:1391	arg1	fragments					1393:1401	109 nadroparin fragments	1378:1401	109 nadroparin fragments	1378:1401	And a total of 94 enoxaparin fragments and 109 nadroparin fragments were detected and identified.
25701653	3	47	theme	rapid	487:491	arg1	method					523:528	a rapid and powerful fragment mapping method	485:528	a rapid and powerful fragment mapping method for structural characterization of LMWHs	485:569	The work presented herein provides a rapid and powerful fragment mapping method for structural characterization of LMWHs.
25701653	9	48	theme	odd	1640:1642	arg1	oligosaccharides					1651:1666	odd number oligosaccharides	1640:1666	many components containing characteristic LMWH structures such as saturated L-idopyranosuronic acid, 2,5-anhydro-D-mannitol, 1,6-anhydro-D-aminopyranose, as well as odd number oligosaccharides	1475:1666	Besides the common LMWH oligosaccharides, many components containing characteristic LMWH structures such as saturated L-idopyranosuronic acid, 2,5-anhydro-D-mannitol, 1,6-anhydro-D-aminopyranose, as well as odd number oligosaccharides were also revealed.
25701653	6	49	contain	containing	1109:1118	arg2	compositions					1147:1158	all theoretical structural compositions	1120:1158	all theoretical structural compositions	1120:1158	A database containing all theoretical structural compositions was established to assist the mass spectra interpretation.
25701653	6	49	contain	containing	1109:1118	arg1	database					1100:1107	A database	1098:1107	A database containing all theoretical structural compositions	1098:1158	A database containing all theoretical structural compositions was established to assist the mass spectra interpretation.
25701653	10	50	theme	Quantitative	1688:1699	arg1	comparison					1701:1710	Quantitative comparison	1688:1710	Quantitative comparison of major components derived from innovator and generic nadroparin products	1688:1785	Quantitative comparison of major components derived from innovator and generic nadroparin products was presented.
25701653	11	51	theme	drugs	1977:1981	arg1	quality					1935:1941	the quality	1931:1941	the quality of this category of anticoagulant drugs	1931:1981	This approach to profile LMWHs' fragments offers a highly reproducible, high resolution and information-rich tool for evaluating the quality of this category of anticoagulant drugs or comparing structural similarities among samples from various sources.
25701653	2	52	theme	clinical	361:368	arg1	LMWHs					331:335	LMWHs	331:335	LMWHs	331:335	Compared to unfractionated heparin (UFH), LMWHs are prevalently used as clinical anticoagulant drugs due to their lower side effects and better bioavailability.
25701653	2	52	theme	clinical	361:368	arg1	drugs					384:388	clinical anticoagulant drugs	361:388	clinical anticoagulant drugs due to their lower side effects and better bioavailability	361:447	Compared to unfractionated heparin (UFH), LMWHs are prevalently used as clinical anticoagulant drugs due to their lower side effects and better bioavailability.
25701653	3	53	theme	LMWHs	565:569	arg1	characterization					545:560	structural characterization	534:560	structural characterization of LMWHs	534:569	The work presented herein provides a rapid and powerful fragment mapping method for structural characterization of LMWHs.
25701653	1	54	theme	heparin	280:286	arg1	depolymerization					260:275	chemical or enzymatic depolymerization	238:275	chemical or enzymatic depolymerization of heparin	238:286	Low molecular weight heparins (LMWHs) are linear and highly charged carbohydrate polymers prepared by chemical or enzymatic depolymerization of heparin.
25701653	7	55	attach	derived	1235:1241	arg2	digests					1227:1233	The six digests	1219:1233	The six digests derived by three enzymes from two types of LMWHs	1219:1282	The six digests derived by three enzymes from two types of LMWHs exhibited distinguishable fingerprinting patterns.
25701653	7	55	attach	derived	1235:1241	arg1	types					1269:1273	two types	1265:1273	two types of LMWHs	1265:1282	The six digests derived by three enzymes from two types of LMWHs exhibited distinguishable fingerprinting patterns.
25701653	4	56	theme	EC	786:787	arg1	#					789:789	no EC #	783:789	no EC # assigned	783:798	The chain fragments of two types of LMWHs, enoxaparin and nadroparin, were generated by controlled enzymatic digestion with each of heparinase I (Hep I, Enzyme Commission (EC) # 4.2.2.7), heparinase II (Hep II, no EC # assigned) and heparinase III (Hep III, EC # 4.2.2.8).
25701653	4	56	theme	EC	786:787	arg1	Hep					775:777	Hep II	775:780	Hep II	775:780	The chain fragments of two types of LMWHs, enoxaparin and nadroparin, were generated by controlled enzymatic digestion with each of heparinase I (Hep I, Enzyme Commission (EC) # 4.2.2.7), heparinase II (Hep II, no EC # assigned) and heparinase III (Hep III, EC # 4.2.2.8).
25701653	11	57	from	sources	2047:2053	arg1	samples					2026:2032	samples	2026:2032	samples from various sources	2026:2053	This approach to profile LMWHs' fragments offers a highly reproducible, high resolution and information-rich tool for evaluating the quality of this category of anticoagulant drugs or comparing structural similarities among samples from various sources.
25701653	4	58	theme	controlled	660:669	arg1	digestion					681:689	controlled enzymatic digestion	660:689	controlled enzymatic digestion with each of heparinase I (Hep I, Enzyme Commission (EC) # 4.2.2.7), heparinase II (Hep II, no EC # assigned) and heparinase III (Hep III, EC # 4.2.2.8)	660:842	The chain fragments of two types of LMWHs, enoxaparin and nadroparin, were generated by controlled enzymatic digestion with each of heparinase I (Hep I, Enzyme Commission (EC) # 4.2.2.7), heparinase II (Hep II, no EC # assigned) and heparinase III (Hep III, EC # 4.2.2.8).
25701653	4	59	dep	Hep	821:823	arg1	EC					830:831	EC # 4.2.2.8	830:841	EC # 4.2.2.8	830:841	The chain fragments of two types of LMWHs, enoxaparin and nadroparin, were generated by controlled enzymatic digestion with each of heparinase I (Hep I, Enzyme Commission (EC) # 4.2.2.7), heparinase II (Hep II, no EC # assigned) and heparinase III (Hep III, EC # 4.2.2.8).
25701653	4	60	dep	heparinase	760:769	arg1	#					789:789	no EC #	783:789	no EC # assigned	783:798	The chain fragments of two types of LMWHs, enoxaparin and nadroparin, were generated by controlled enzymatic digestion with each of heparinase I (Hep I, Enzyme Commission (EC) # 4.2.2.7), heparinase II (Hep II, no EC # assigned) and heparinase III (Hep III, EC # 4.2.2.8).
25701653	4	60	dep	heparinase	760:769	arg1	Hep					775:777	Hep II	775:780	Hep II	775:780	The chain fragments of two types of LMWHs, enoxaparin and nadroparin, were generated by controlled enzymatic digestion with each of heparinase I (Hep I, Enzyme Commission (EC) # 4.2.2.7), heparinase II (Hep II, no EC # assigned) and heparinase III (Hep III, EC # 4.2.2.8).
25701653	1	61	theme	charged	196:202	arg1	polymers					217:224	linear and highly charged carbohydrate polymers	178:224	linear and highly charged carbohydrate polymers prepared by chemical or enzymatic depolymerization of heparin	178:286	Low molecular weight heparins (LMWHs) are linear and highly charged carbohydrate polymers prepared by chemical or enzymatic depolymerization of heparin.
25701653	1	61	theme	charged	196:202	arg1	heparins					157:164	Low molecular weight heparins	136:164	Low molecular weight heparins (LMWHs)	136:172	Low molecular weight heparins (LMWHs) are linear and highly charged carbohydrate polymers prepared by chemical or enzymatic depolymerization of heparin.
25701653	4	62	theme	#	833:833	arg1	EC					830:831	EC # 4.2.2.8	830:841	EC # 4.2.2.8	830:841	The chain fragments of two types of LMWHs, enoxaparin and nadroparin, were generated by controlled enzymatic digestion with each of heparinase I (Hep I, Enzyme Commission (EC) # 4.2.2.7), heparinase II (Hep II, no EC # assigned) and heparinase III (Hep III, EC # 4.2.2.8).
25701653	3	63	theme	mapping	515:521	arg1	method					523:528	a rapid and powerful fragment mapping method	485:528	a rapid and powerful fragment mapping method for structural characterization of LMWHs	485:569	The work presented herein provides a rapid and powerful fragment mapping method for structural characterization of LMWHs.
25701653	11	64	theme	information-rich	1894:1909	arg1	tool					1911:1914	a highly reproducible, high resolution and information-rich tool	1851:1914	a highly reproducible, high resolution and information-rich tool for evaluating the quality of this category of anticoagulant drugs or comparing structural similarities among samples from various sources	1851:2053	This approach to profile LMWHs' fragments offers a highly reproducible, high resolution and information-rich tool for evaluating the quality of this category of anticoagulant drugs or comparing structural similarities among samples from various sources.
25701653	9	65	theme	common	1445:1450	arg1	oligosaccharides					1457:1472	the common LMWH oligosaccharides	1441:1472	the common LMWH oligosaccharides	1441:1472	Besides the common LMWH oligosaccharides, many components containing characteristic LMWH structures such as saturated L-idopyranosuronic acid, 2,5-anhydro-D-mannitol, 1,6-anhydro-D-aminopyranose, as well as odd number oligosaccharides were also revealed.
25701653	11	66	theme	structural	1996:2005	arg1	similarities					2007:2018	structural similarities	1996:2018	structural similarities among samples from various sources	1996:2053	This approach to profile LMWHs' fragments offers a highly reproducible, high resolution and information-rich tool for evaluating the quality of this category of anticoagulant drugs or comparing structural similarities among samples from various sources.
25701653	10	67	theme	nadroparin	1767:1776	arg1	products					1778:1785	innovator and generic nadroparin products	1745:1785	innovator and generic nadroparin products	1745:1785	Quantitative comparison of major components derived from innovator and generic nadroparin products was presented.
25701653	11	68	theme	reproducible	1860:1871	arg1	tool					1911:1914	a highly reproducible, high resolution and information-rich tool	1851:1914	a highly reproducible, high resolution and information-rich tool for evaluating the quality of this category of anticoagulant drugs or comparing structural similarities among samples from various sources	1851:2053	This approach to profile LMWHs' fragments offers a highly reproducible, high resolution and information-rich tool for evaluating the quality of this category of anticoagulant drugs or comparing structural similarities among samples from various sources.
25701653	5	69	theme	mass	970:973	arg1	spectrometry					975:986	electrospray ion trap time-of-flight mass spectrometry	933:986	electrospray ion trap time-of-flight mass spectrometry (ESI-IT-TOF-MS)	933:1002	Reversed phase ion pair high performance liquid chromatography (RPIP-HPLC) coupled with electrospray ion trap time-of-flight mass spectrometry (ESI-IT-TOF-MS) was used to profile the oligosaccharide chains ranging from disaccharides to decasaccharides.
25701653	5	69	theme	mass	970:973	arg1	ESI-IT-TOF-MS					989:1001	ESI-IT-TOF-MS	989:1001	ESI-IT-TOF-MS	989:1001	Reversed phase ion pair high performance liquid chromatography (RPIP-HPLC) coupled with electrospray ion trap time-of-flight mass spectrometry (ESI-IT-TOF-MS) was used to profile the oligosaccharide chains ranging from disaccharides to decasaccharides.
25701653	1	70	theme	molecular	140:148	arg1	polymers					217:224	linear and highly charged carbohydrate polymers	178:224	linear and highly charged carbohydrate polymers prepared by chemical or enzymatic depolymerization of heparin	178:286	Low molecular weight heparins (LMWHs) are linear and highly charged carbohydrate polymers prepared by chemical or enzymatic depolymerization of heparin.
25701653	1	70	theme	molecular	140:148	arg1	LMWHs					167:171	LMWHs	167:171	LMWHs	167:171	Low molecular weight heparins (LMWHs) are linear and highly charged carbohydrate polymers prepared by chemical or enzymatic depolymerization of heparin.
25701653	1	70	theme	molecular	140:148	arg1	heparins					157:164	Low molecular weight heparins	136:164	Low molecular weight heparins (LMWHs)	136:172	Low molecular weight heparins (LMWHs) are linear and highly charged carbohydrate polymers prepared by chemical or enzymatic depolymerization of heparin.
25701653	4	71	dep	I	715:715	arg1	#					748:748	Enzyme Commission (EC) # 4.2.2.7	725:756	Enzyme Commission (EC) # 4.2.2.7	725:756	The chain fragments of two types of LMWHs, enoxaparin and nadroparin, were generated by controlled enzymatic digestion with each of heparinase I (Hep I, Enzyme Commission (EC) # 4.2.2.7), heparinase II (Hep II, no EC # assigned) and heparinase III (Hep III, EC # 4.2.2.8).
25701653	4	71	dep	I	715:715	arg1	I					722:722	Hep I	718:722	Hep I	718:722	The chain fragments of two types of LMWHs, enoxaparin and nadroparin, were generated by controlled enzymatic digestion with each of heparinase I (Hep I, Enzyme Commission (EC) # 4.2.2.7), heparinase II (Hep II, no EC # assigned) and heparinase III (Hep III, EC # 4.2.2.8).
25701653	11	72	theme	resolution	1879:1888	arg1	tool					1911:1914	a highly reproducible, high resolution and information-rich tool	1851:1914	a highly reproducible, high resolution and information-rich tool for evaluating the quality of this category of anticoagulant drugs or comparing structural similarities among samples from various sources	1851:2053	This approach to profile LMWHs' fragments offers a highly reproducible, high resolution and information-rich tool for evaluating the quality of this category of anticoagulant drugs or comparing structural similarities among samples from various sources.
25701653	0	73	theme	phase	67:71	arg1	spectrometry					122:133	reversed phase ion pair liquid chromatography-electrospray mass spectrometry	58:133	reversed phase ion pair liquid chromatography-electrospray mass spectrometry	58:133	Fragment profiling of low molecular weight heparins using reversed phase ion pair liquid chromatography-electrospray mass spectrometry.
25701653	6	74	theme	structural	1136:1145	arg1	compositions					1147:1158	all theoretical structural compositions	1120:1158	all theoretical structural compositions	1120:1158	A database containing all theoretical structural compositions was established to assist the mass spectra interpretation.
25701653	0	75	theme	pair	77:80	arg1	spectrometry					122:133	reversed phase ion pair liquid chromatography-electrospray mass spectrometry	58:133	reversed phase ion pair liquid chromatography-electrospray mass spectrometry	58:133	Fragment profiling of low molecular weight heparins using reversed phase ion pair liquid chromatography-electrospray mass spectrometry.
25701653	10	76	attach	derived	1732:1738	arg2	components					1721:1730	major components	1715:1730	major components derived from innovator and generic nadroparin products	1715:1785	Quantitative comparison of major components derived from innovator and generic nadroparin products was presented.
25701653	10	76	attach	derived	1732:1738	arg1	products					1778:1785	innovator and generic nadroparin products	1745:1785	innovator and generic nadroparin products	1745:1785	Quantitative comparison of major components derived from innovator and generic nadroparin products was presented.
25701653	9	77	theme	LMWH	1517:1520	arg1	acid					1570:1573	saturated L-idopyranosuronic acid	1541:1573	saturated L-idopyranosuronic acid	1541:1573	Besides the common LMWH oligosaccharides, many components containing characteristic LMWH structures such as saturated L-idopyranosuronic acid, 2,5-anhydro-D-mannitol, 1,6-anhydro-D-aminopyranose, as well as odd number oligosaccharides were also revealed.
25701653	9	77	theme	LMWH	1517:1520	arg1	structures					1522:1531	characteristic LMWH structures	1502:1531	characteristic LMWH structures such as saturated L-idopyranosuronic acid, 2,5-anhydro-D-mannitol, 1,6-anhydro-D-aminopyranose,	1502:1627	Besides the common LMWH oligosaccharides, many components containing characteristic LMWH structures such as saturated L-idopyranosuronic acid, 2,5-anhydro-D-mannitol, 1,6-anhydro-D-aminopyranose, as well as odd number oligosaccharides were also revealed.
25701653	4	78	theme	heparinase	704:713	arg1	I					715:715	heparinase I	704:715	heparinase I (Hep I, Enzyme Commission (EC) # 4.2.2.7)	704:757	The chain fragments of two types of LMWHs, enoxaparin and nadroparin, were generated by controlled enzymatic digestion with each of heparinase I (Hep I, Enzyme Commission (EC) # 4.2.2.7), heparinase II (Hep II, no EC # assigned) and heparinase III (Hep III, EC # 4.2.2.8).
25701653	0	79	theme	chromatography-electrospray	89:115	arg1	spectrometry					122:133	reversed phase ion pair liquid chromatography-electrospray mass spectrometry	58:133	reversed phase ion pair liquid chromatography-electrospray mass spectrometry	58:133	Fragment profiling of low molecular weight heparins using reversed phase ion pair liquid chromatography-electrospray mass spectrometry.
25701653	5	80	theme	high	869:872	arg1	RPIP-HPLC					909:917	RPIP-HPLC	909:917	RPIP-HPLC	909:917	Reversed phase ion pair high performance liquid chromatography (RPIP-HPLC) coupled with electrospray ion trap time-of-flight mass spectrometry (ESI-IT-TOF-MS) was used to profile the oligosaccharide chains ranging from disaccharides to decasaccharides.
25701653	5	80	theme	high	869:872	arg1	chromatography					893:906	Reversed phase ion pair high performance liquid chromatography	845:906	Reversed phase ion pair high performance liquid chromatography (RPIP-HPLC) coupled with electrospray ion trap time-of-flight mass spectrometry (ESI-IT-TOF-MS)	845:1002	Reversed phase ion pair high performance liquid chromatography (RPIP-HPLC) coupled with electrospray ion trap time-of-flight mass spectrometry (ESI-IT-TOF-MS) was used to profile the oligosaccharide chains ranging from disaccharides to decasaccharides.
25701653	10	81	theme	components	1721:1730	arg1	comparison					1701:1710	Quantitative comparison	1688:1710	Quantitative comparison of major components derived from innovator and generic nadroparin products	1688:1785	Quantitative comparison of major components derived from innovator and generic nadroparin products was presented.
25701653	5	82	theme	liquid	886:891	arg1	RPIP-HPLC					909:917	RPIP-HPLC	909:917	RPIP-HPLC	909:917	Reversed phase ion pair high performance liquid chromatography (RPIP-HPLC) coupled with electrospray ion trap time-of-flight mass spectrometry (ESI-IT-TOF-MS) was used to profile the oligosaccharide chains ranging from disaccharides to decasaccharides.
25701653	5	82	theme	liquid	886:891	arg1	chromatography					893:906	Reversed phase ion pair high performance liquid chromatography	845:906	Reversed phase ion pair high performance liquid chromatography (RPIP-HPLC) coupled with electrospray ion trap time-of-flight mass spectrometry (ESI-IT-TOF-MS)	845:1002	Reversed phase ion pair high performance liquid chromatography (RPIP-HPLC) coupled with electrospray ion trap time-of-flight mass spectrometry (ESI-IT-TOF-MS) was used to profile the oligosaccharide chains ranging from disaccharides to decasaccharides.
25701653	7	83	theme	LMWHs	1278:1282	arg1	types					1269:1273	two types	1265:1273	two types of LMWHs	1265:1282	The six digests derived by three enzymes from two types of LMWHs exhibited distinguishable fingerprinting patterns.
25701653	5	84	theme	oligosaccharide	1028:1042	arg1	chains					1044:1049	the oligosaccharide chains	1024:1049	the oligosaccharide chains ranging from disaccharides to decasaccharides	1024:1095	Reversed phase ion pair high performance liquid chromatography (RPIP-HPLC) coupled with electrospray ion trap time-of-flight mass spectrometry (ESI-IT-TOF-MS) was used to profile the oligosaccharide chains ranging from disaccharides to decasaccharides.
25701653	5	85	theme	Reversed	845:852	arg1	RPIP-HPLC					909:917	RPIP-HPLC	909:917	RPIP-HPLC	909:917	Reversed phase ion pair high performance liquid chromatography (RPIP-HPLC) coupled with electrospray ion trap time-of-flight mass spectrometry (ESI-IT-TOF-MS) was used to profile the oligosaccharide chains ranging from disaccharides to decasaccharides.
25701653	5	85	theme	Reversed	845:852	arg1	chromatography					893:906	Reversed phase ion pair high performance liquid chromatography	845:906	Reversed phase ion pair high performance liquid chromatography (RPIP-HPLC) coupled with electrospray ion trap time-of-flight mass spectrometry (ESI-IT-TOF-MS)	845:1002	Reversed phase ion pair high performance liquid chromatography (RPIP-HPLC) coupled with electrospray ion trap time-of-flight mass spectrometry (ESI-IT-TOF-MS) was used to profile the oligosaccharide chains ranging from disaccharides to decasaccharides.
25701653	4	86	theme	chain	576:580	arg1	enoxaparin					615:624	enoxaparin	615:624	enoxaparin	615:624	The chain fragments of two types of LMWHs, enoxaparin and nadroparin, were generated by controlled enzymatic digestion with each of heparinase I (Hep I, Enzyme Commission (EC) # 4.2.2.7), heparinase II (Hep II, no EC # assigned) and heparinase III (Hep III, EC # 4.2.2.8).
25701653	4	86	theme	chain	576:580	arg1	fragments					582:590	The chain fragments	572:590	The chain fragments of two types of LMWHs	572:612	The chain fragments of two types of LMWHs, enoxaparin and nadroparin, were generated by controlled enzymatic digestion with each of heparinase I (Hep I, Enzyme Commission (EC) # 4.2.2.7), heparinase II (Hep II, no EC # assigned) and heparinase III (Hep III, EC # 4.2.2.8).
25701653	4	86	theme	chain	576:580	arg1	nadroparin					630:639	nadroparin	630:639	nadroparin	630:639	The chain fragments of two types of LMWHs, enoxaparin and nadroparin, were generated by controlled enzymatic digestion with each of heparinase I (Hep I, Enzyme Commission (EC) # 4.2.2.7), heparinase II (Hep II, no EC # assigned) and heparinase III (Hep III, EC # 4.2.2.8).
25701653	4	87	theme	Enzyme	725:730	arg1	#					748:748	Enzyme Commission (EC) # 4.2.2.7	725:756	Enzyme Commission (EC) # 4.2.2.7	725:756	The chain fragments of two types of LMWHs, enoxaparin and nadroparin, were generated by controlled enzymatic digestion with each of heparinase I (Hep I, Enzyme Commission (EC) # 4.2.2.7), heparinase II (Hep II, no EC # assigned) and heparinase III (Hep III, EC # 4.2.2.8).
25701653	4	87	theme	Enzyme	725:730	arg1	I					722:722	Hep I	718:722	Hep I	718:722	The chain fragments of two types of LMWHs, enoxaparin and nadroparin, were generated by controlled enzymatic digestion with each of heparinase I (Hep I, Enzyme Commission (EC) # 4.2.2.7), heparinase II (Hep II, no EC # assigned) and heparinase III (Hep III, EC # 4.2.2.8).
25701653	2	88	theme	due	390:392	arg1	LMWHs					331:335	LMWHs	331:335	LMWHs	331:335	Compared to unfractionated heparin (UFH), LMWHs are prevalently used as clinical anticoagulant drugs due to their lower side effects and better bioavailability.
25701653	2	88	theme	due	390:392	arg1	drugs					384:388	clinical anticoagulant drugs	361:388	clinical anticoagulant drugs due to their lower side effects and better bioavailability	361:447	Compared to unfractionated heparin (UFH), LMWHs are prevalently used as clinical anticoagulant drugs due to their lower side effects and better bioavailability.
25701653	0	89	theme	low	22:24	arg1	heparins					43:50	low molecular weight heparins	22:50	low molecular weight heparins	22:50	Fragment profiling of low molecular weight heparins using reversed phase ion pair liquid chromatography-electrospray mass spectrometry.
25701653	5	90	theme	ion	860:862	arg1	RPIP-HPLC					909:917	RPIP-HPLC	909:917	RPIP-HPLC	909:917	Reversed phase ion pair high performance liquid chromatography (RPIP-HPLC) coupled with electrospray ion trap time-of-flight mass spectrometry (ESI-IT-TOF-MS) was used to profile the oligosaccharide chains ranging from disaccharides to decasaccharides.
25701653	5	90	theme	ion	860:862	arg1	chromatography					893:906	Reversed phase ion pair high performance liquid chromatography	845:906	Reversed phase ion pair high performance liquid chromatography (RPIP-HPLC) coupled with electrospray ion trap time-of-flight mass spectrometry (ESI-IT-TOF-MS)	845:1002	Reversed phase ion pair high performance liquid chromatography (RPIP-HPLC) coupled with electrospray ion trap time-of-flight mass spectrometry (ESI-IT-TOF-MS) was used to profile the oligosaccharide chains ranging from disaccharides to decasaccharides.
25701653	9	91	theme	saturated	1541:1549	arg1	1,6-anhydro-D-aminopyranose					1600:1626	1,6-anhydro-D-aminopyranose	1600:1626	1,6-anhydro-D-aminopyranose	1600:1626	Besides the common LMWH oligosaccharides, many components containing characteristic LMWH structures such as saturated L-idopyranosuronic acid, 2,5-anhydro-D-mannitol, 1,6-anhydro-D-aminopyranose, as well as odd number oligosaccharides were also revealed.
25701653	9	91	theme	saturated	1541:1549	arg1	2,5-anhydro-D-mannitol					1576:1597	2,5-anhydro-D-mannitol	1576:1597	2,5-anhydro-D-mannitol	1576:1597	Besides the common LMWH oligosaccharides, many components containing characteristic LMWH structures such as saturated L-idopyranosuronic acid, 2,5-anhydro-D-mannitol, 1,6-anhydro-D-aminopyranose, as well as odd number oligosaccharides were also revealed.
25701653	9	91	theme	saturated	1541:1549	arg1	acid					1570:1573	saturated L-idopyranosuronic acid	1541:1573	saturated L-idopyranosuronic acid	1541:1573	Besides the common LMWH oligosaccharides, many components containing characteristic LMWH structures such as saturated L-idopyranosuronic acid, 2,5-anhydro-D-mannitol, 1,6-anhydro-D-aminopyranose, as well as odd number oligosaccharides were also revealed.
25701653	0	92	theme	weight	36:41	arg1	heparins					43:50	low molecular weight heparins	22:50	low molecular weight heparins	22:50	Fragment profiling of low molecular weight heparins using reversed phase ion pair liquid chromatography-electrospray mass spectrometry.
25701653	11	93	theme	category	1951:1958	arg1	quality					1935:1941	the quality	1931:1941	the quality of this category of anticoagulant drugs	1931:1981	This approach to profile LMWHs' fragments offers a highly reproducible, high resolution and information-rich tool for evaluating the quality of this category of anticoagulant drugs or comparing structural similarities among samples from various sources.
25701653	2	94	theme	side	409:412	arg1	effects					414:420	their lower side effects	397:420	their lower side effects	397:420	Compared to unfractionated heparin (UFH), LMWHs are prevalently used as clinical anticoagulant drugs due to their lower side effects and better bioavailability.
25701653	5	95	theme	ion	946:948	arg1	spectrometry					975:986	electrospray ion trap time-of-flight mass spectrometry	933:986	electrospray ion trap time-of-flight mass spectrometry (ESI-IT-TOF-MS)	933:1002	Reversed phase ion pair high performance liquid chromatography (RPIP-HPLC) coupled with electrospray ion trap time-of-flight mass spectrometry (ESI-IT-TOF-MS) was used to profile the oligosaccharide chains ranging from disaccharides to decasaccharides.
25701653	5	95	theme	ion	946:948	arg1	ESI-IT-TOF-MS					989:1001	ESI-IT-TOF-MS	989:1001	ESI-IT-TOF-MS	989:1001	Reversed phase ion pair high performance liquid chromatography (RPIP-HPLC) coupled with electrospray ion trap time-of-flight mass spectrometry (ESI-IT-TOF-MS) was used to profile the oligosaccharide chains ranging from disaccharides to decasaccharides.
25701653	8	96	theme	fragments	1364:1372	arg1	total					1341:1345	a total	1339:1345	a total of 94 enoxaparin fragments and 109 nadroparin fragments	1339:1401	And a total of 94 enoxaparin fragments and 109 nadroparin fragments were detected and identified.
25701653	3	97	theme	fragment	506:513	arg1	method					523:528	a rapid and powerful fragment mapping method	485:528	a rapid and powerful fragment mapping method for structural characterization of LMWHs	485:569	The work presented herein provides a rapid and powerful fragment mapping method for structural characterization of LMWHs.
25701653	9	98	contain	containing	1491:1500	arg2	structures					1522:1531	characteristic LMWH structures	1502:1531	characteristic LMWH structures such as saturated L-idopyranosuronic acid, 2,5-anhydro-D-mannitol, 1,6-anhydro-D-aminopyranose,	1502:1627	Besides the common LMWH oligosaccharides, many components containing characteristic LMWH structures such as saturated L-idopyranosuronic acid, 2,5-anhydro-D-mannitol, 1,6-anhydro-D-aminopyranose, as well as odd number oligosaccharides were also revealed.
25701653	9	98	contain	containing	1491:1500	arg1	components					1480:1489	many components	1475:1489	many components containing characteristic LMWH structures such as saturated L-idopyranosuronic acid, 2,5-anhydro-D-mannitol, 1,6-anhydro-D-aminopyranose, as well as odd number oligosaccharides	1475:1666	Besides the common LMWH oligosaccharides, many components containing characteristic LMWH structures such as saturated L-idopyranosuronic acid, 2,5-anhydro-D-mannitol, 1,6-anhydro-D-aminopyranose, as well as odd number oligosaccharides were also revealed.
25701653	9	98	contain	containing	1491:1500	arg2	acid					1570:1573	saturated L-idopyranosuronic acid	1541:1573	saturated L-idopyranosuronic acid	1541:1573	Besides the common LMWH oligosaccharides, many components containing characteristic LMWH structures such as saturated L-idopyranosuronic acid, 2,5-anhydro-D-mannitol, 1,6-anhydro-D-aminopyranose, as well as odd number oligosaccharides were also revealed.
25701653	2	99	theme	anticoagulant	370:382	arg1	LMWHs					331:335	LMWHs	331:335	LMWHs	331:335	Compared to unfractionated heparin (UFH), LMWHs are prevalently used as clinical anticoagulant drugs due to their lower side effects and better bioavailability.
25701653	2	99	theme	anticoagulant	370:382	arg1	drugs					384:388	clinical anticoagulant drugs	361:388	clinical anticoagulant drugs due to their lower side effects and better bioavailability	361:447	Compared to unfractionated heparin (UFH), LMWHs are prevalently used as clinical anticoagulant drugs due to their lower side effects and better bioavailability.
25701653	1	100	theme	chemical	238:245	arg1	depolymerization					260:275	chemical or enzymatic depolymerization	238:275	chemical or enzymatic depolymerization of heparin	238:286	Low molecular weight heparins (LMWHs) are linear and highly charged carbohydrate polymers prepared by chemical or enzymatic depolymerization of heparin.
25701653	9	101	theme	number	1644:1649	arg1	oligosaccharides					1651:1666	odd number oligosaccharides	1640:1666	many components containing characteristic LMWH structures such as saturated L-idopyranosuronic acid, 2,5-anhydro-D-mannitol, 1,6-anhydro-D-aminopyranose, as well as odd number oligosaccharides	1475:1666	Besides the common LMWH oligosaccharides, many components containing characteristic LMWH structures such as saturated L-idopyranosuronic acid, 2,5-anhydro-D-mannitol, 1,6-anhydro-D-aminopyranose, as well as odd number oligosaccharides were also revealed.
25701653	10	102	theme	generic	1759:1765	arg1	products					1778:1785	innovator and generic nadroparin products	1745:1785	innovator and generic nadroparin products	1745:1785	Quantitative comparison of major components derived from innovator and generic nadroparin products was presented.
25701653	8	103	theme	fragments	1393:1401	arg1	total					1341:1345	a total	1339:1345	a total of 94 enoxaparin fragments and 109 nadroparin fragments	1339:1401	And a total of 94 enoxaparin fragments and 109 nadroparin fragments were detected and identified.
25701653	3	104	theme	structural	534:543	arg1	characterization					545:560	structural characterization	534:560	structural characterization of LMWHs	534:569	The work presented herein provides a rapid and powerful fragment mapping method for structural characterization of LMWHs.
25701653	1	105	theme	enzymatic	250:258	arg1	depolymerization					260:275	chemical or enzymatic depolymerization	238:275	chemical or enzymatic depolymerization of heparin	238:286	Low molecular weight heparins (LMWHs) are linear and highly charged carbohydrate polymers prepared by chemical or enzymatic depolymerization of heparin.
26453848	7	0	theme	Luedeking-Piret	976:990	arg1	equation					992:999	microbial growth and Luedeking-Piret equation	955:999	microbial growth and Luedeking-Piret equation for product formation and substrate utilization	955:1047	Logistic model for microbial growth and Luedeking-Piret equation for product formation and substrate utilization were found to fit the experiment.
26453848	1	1	theme	rhizosphere	173:183	arg1	soil					185:188	rhizosphere soil	173:188	rhizosphere soil	173:188	Pseudomonas fluorescens, isolated from rhizosphere soil, was exploited for the production of exopolysaccharide (EPS).
26453848	0	2	theme	rhizosphere	83:93	arg1	isolate					95:101	rhizosphere isolate	83:101	rhizosphere isolate	83:101	Optimization, kinetics and antioxidant activity of exopolysaccharide produced from rhizosphere isolate, Pseudomonas fluorescens CrN6.
26453848	0	2	theme	rhizosphere	83:93	arg1	CrN6					128:131	Pseudomonas fluorescens CrN6	104:131	Pseudomonas fluorescens CrN6	104:131	Optimization, kinetics and antioxidant activity of exopolysaccharide produced from rhizosphere isolate, Pseudomonas fluorescens CrN6.
26453848	4	3	theme	rice	501:504	arg1	bran					506:509	rice bran	501:509	rice bran	501:509	Plackett-Burman statistical design was applied to evaluate the selected sixteen components from which, rice bran, peptone, NaCl and MnCl2 were found to be effective and significant on the fermentation process.
26453848	10	4	theme	pharmaceutical	1388:1401	arg1	applications					1403:1414	various industrial and pharmaceutical applications	1365:1414	various industrial and pharmaceutical applications	1365:1414	The extracted polysaccharide could thus be ecofriendly due to its biodegradability and nontoxicity, and subjected to various industrial and pharmaceutical applications.
26453848	0	5	theme	Pseudomonas	104:114	arg1	isolate					95:101	rhizosphere isolate	83:101	rhizosphere isolate	83:101	Optimization, kinetics and antioxidant activity of exopolysaccharide produced from rhizosphere isolate, Pseudomonas fluorescens CrN6.
26453848	0	5	theme	Pseudomonas	104:114	arg1	CrN6					128:131	Pseudomonas fluorescens CrN6	104:131	Pseudomonas fluorescens CrN6	104:131	Optimization, kinetics and antioxidant activity of exopolysaccharide produced from rhizosphere isolate, Pseudomonas fluorescens CrN6.
26453848	4	6	theme	selected	461:468	arg1	components					478:487	the selected sixteen components	457:487	the selected sixteen components from which, rice bran, peptone, NaCl and MnCl2 were found to be effective and significant on the fermentation process	457:605	Plackett-Burman statistical design was applied to evaluate the selected sixteen components from which, rice bran, peptone, NaCl and MnCl2 were found to be effective and significant on the fermentation process.
26453848	10	7	theme	industrial	1373:1382	arg1	applications					1403:1414	various industrial and pharmaceutical applications	1365:1414	various industrial and pharmaceutical applications	1365:1414	The extracted polysaccharide could thus be ecofriendly due to its biodegradability and nontoxicity, and subjected to various industrial and pharmaceutical applications.
26453848	6	8	theme	process	916:922	arg1	parameters					924:933	the process parameters	912:933	the process parameters	912:933	Kinetic modeling was also performed to simulate the process parameters.
26453848	5	9	theme	response	699:706	arg1	plots					716:720	response surface plots	699:720	response surface plots	699:720	To study the concentration of each component, central composite design was carried out and response surface plots indicated that the following concentrations significantly enhanced the production - rice bran 5.02%, peptone 0.35%, NaCl 0.51%, MnCl2 0.074%.
26453848	1	10	attach	isolated	159:166	arg1	soil					185:188	rhizosphere soil	173:188	rhizosphere soil	173:188	Pseudomonas fluorescens, isolated from rhizosphere soil, was exploited for the production of exopolysaccharide (EPS).
26453848	1	10	attach	isolated	159:166	arg2	fluorescens					146:156	Pseudomonas fluorescens	134:156	Pseudomonas fluorescens	134:156	Pseudomonas fluorescens, isolated from rhizosphere soil, was exploited for the production of exopolysaccharide (EPS).
26453848	0	11	dep	Pseudomonas	104:114	arg1	fluorescens					116:126	fluorescens	116:126	fluorescens	116:126	Optimization, kinetics and antioxidant activity of exopolysaccharide produced from rhizosphere isolate, Pseudomonas fluorescens CrN6.
26453848	5	12	theme	peptone	823:829	arg1	%					820:820	rice bran 5.02%	806:820	rice bran 5.02%	806:820	To study the concentration of each component, central composite design was carried out and response surface plots indicated that the following concentrations significantly enhanced the production - rice bran 5.02%, peptone 0.35%, NaCl 0.51%, MnCl2 0.074%.
26453848	5	12	theme	peptone	823:829	arg1	%					835:835	peptone 0.35%	823:835	peptone 0.35%	823:835	To study the concentration of each component, central composite design was carried out and response surface plots indicated that the following concentrations significantly enhanced the production - rice bran 5.02%, peptone 0.35%, NaCl 0.51%, MnCl2 0.074%.
26453848	5	13	theme	surface	708:714	arg1	plots					716:720	response surface plots	699:720	response surface plots	699:720	To study the concentration of each component, central composite design was carried out and response surface plots indicated that the following concentrations significantly enhanced the production - rice bran 5.02%, peptone 0.35%, NaCl 0.51%, MnCl2 0.074%.
26453848	8	14	from	48h	1158:1160	arg1	EPS/L					1149:1153	EPS/L	1149:1153	EPS/L at 48h	1149:1160	The present investigation resulted in a maximum yield of 4.62g of EPS/L at 48h.
26453848	8	14	from	48h	1158:1160	arg1	4.62g					1140:1144	4.62g	1140:1144	4.62g of EPS/L at 48h	1140:1160	The present investigation resulted in a maximum yield of 4.62g of EPS/L at 48h.
26453848	10	15	theme	various	1365:1371	arg1	applications					1403:1414	various industrial and pharmaceutical applications	1365:1414	various industrial and pharmaceutical applications	1365:1414	The extracted polysaccharide could thus be ecofriendly due to its biodegradability and nontoxicity, and subjected to various industrial and pharmaceutical applications.
26453848	8	16	theme	4.62g	1140:1144	arg1	yield					1131:1135	a maximum yield	1121:1135	a maximum yield of 4.62g of EPS/L at 48h	1121:1160	The present investigation resulted in a maximum yield of 4.62g of EPS/L at 48h.
26453848	8	17	from	4.62g	1140:1144	arg1	48h					1158:1160	48h	1158:1160	48h	1158:1160	The present investigation resulted in a maximum yield of 4.62g of EPS/L at 48h.
26453848	10	18	theme	extracted	1252:1260	arg1	due					1303:1305	due	1303:1305	due	1303:1305	The extracted polysaccharide could thus be ecofriendly due to its biodegradability and nontoxicity, and subjected to various industrial and pharmaceutical applications.
26453848	10	18	theme	extracted	1252:1260	arg1	polysaccharide					1262:1275	The extracted polysaccharide	1248:1275	The extracted polysaccharide	1248:1275	The extracted polysaccharide could thus be ecofriendly due to its biodegradability and nontoxicity, and subjected to various industrial and pharmaceutical applications.
26453848	8	19	theme	EPS/L	1149:1153	arg1	4.62g					1140:1144	4.62g	1140:1144	4.62g of EPS/L at 48h	1140:1160	The present investigation resulted in a maximum yield of 4.62g of EPS/L at 48h.
26453848	8	20	theme	maximum	1123:1129	arg1	yield					1131:1135	a maximum yield	1121:1135	a maximum yield of 4.62g of EPS/L at 48h	1121:1160	The present investigation resulted in a maximum yield of 4.62g of EPS/L at 48h.
26453848	5	21	theme	composite	662:670	arg1	design					672:677	central composite design	654:677	central composite design	654:677	To study the concentration of each component, central composite design was carried out and response surface plots indicated that the following concentrations significantly enhanced the production - rice bran 5.02%, peptone 0.35%, NaCl 0.51%, MnCl2 0.074%.
26453848	9	22	theme	High	1163:1166	arg1	ability					1184:1190	High DPPH scavenging ability	1163:1190	High DPPH scavenging ability	1163:1190	High DPPH scavenging ability was a positive indication to use EPS as an antioxidant.
26453848	9	22	theme	High	1163:1166	arg1	indication					1207:1216	a positive indication	1196:1216	a positive indication to use EPS as an antioxidant	1196:1245	High DPPH scavenging ability was a positive indication to use EPS as an antioxidant.
26453848	2	23	theme	EPS	301:303	arg1	yield					292:296	the yield	288:296	the yield of EPS	288:303	A medium was constituted to enhance the yield of EPS.
26453848	5	24	theme	bran	811:814	arg1	%					820:820	rice bran 5.02%	806:820	rice bran 5.02%	806:820	To study the concentration of each component, central composite design was carried out and response surface plots indicated that the following concentrations significantly enhanced the production - rice bran 5.02%, peptone 0.35%, NaCl 0.51%, MnCl2 0.074%.
26453848	5	24	theme	bran	811:814	arg1	%					847:847	NaCl 0.51%	838:847	NaCl 0.51%	838:847	To study the concentration of each component, central composite design was carried out and response surface plots indicated that the following concentrations significantly enhanced the production - rice bran 5.02%, peptone 0.35%, NaCl 0.51%, MnCl2 0.074%.
26453848	5	24	theme	bran	811:814	arg1	%					835:835	peptone 0.35%	823:835	peptone 0.35%	823:835	To study the concentration of each component, central composite design was carried out and response surface plots indicated that the following concentrations significantly enhanced the production - rice bran 5.02%, peptone 0.35%, NaCl 0.51%, MnCl2 0.074%.
26453848	5	24	theme	bran	811:814	arg1	%					861:861	MnCl2 0.074%	850:861	MnCl2 0.074%	850:861	To study the concentration of each component, central composite design was carried out and response surface plots indicated that the following concentrations significantly enhanced the production - rice bran 5.02%, peptone 0.35%, NaCl 0.51%, MnCl2 0.074%.
26453848	5	25	theme	rice	806:809	arg1	%					820:820	rice bran 5.02%	806:820	rice bran 5.02%	806:820	To study the concentration of each component, central composite design was carried out and response surface plots indicated that the following concentrations significantly enhanced the production - rice bran 5.02%, peptone 0.35%, NaCl 0.51%, MnCl2 0.074%.
26453848	5	25	theme	rice	806:809	arg1	%					847:847	NaCl 0.51%	838:847	NaCl 0.51%	838:847	To study the concentration of each component, central composite design was carried out and response surface plots indicated that the following concentrations significantly enhanced the production - rice bran 5.02%, peptone 0.35%, NaCl 0.51%, MnCl2 0.074%.
26453848	5	25	theme	rice	806:809	arg1	%					835:835	peptone 0.35%	823:835	peptone 0.35%	823:835	To study the concentration of each component, central composite design was carried out and response surface plots indicated that the following concentrations significantly enhanced the production - rice bran 5.02%, peptone 0.35%, NaCl 0.51%, MnCl2 0.074%.
26453848	5	25	theme	rice	806:809	arg1	%					861:861	MnCl2 0.074%	850:861	MnCl2 0.074%	850:861	To study the concentration of each component, central composite design was carried out and response surface plots indicated that the following concentrations significantly enhanced the production - rice bran 5.02%, peptone 0.35%, NaCl 0.51%, MnCl2 0.074%.
26453848	7	26	theme	substrate	1027:1035	arg1	utilization					1037:1047	substrate utilization	1027:1047	substrate utilization	1027:1047	Logistic model for microbial growth and Luedeking-Piret equation for product formation and substrate utilization were found to fit the experiment.
26453848	9	27	theme	DPPH	1168:1171	arg1	ability					1184:1190	High DPPH scavenging ability	1163:1190	High DPPH scavenging ability	1163:1190	High DPPH scavenging ability was a positive indication to use EPS as an antioxidant.
26453848	9	27	theme	DPPH	1168:1171	arg1	indication					1207:1216	a positive indication	1196:1216	a positive indication to use EPS as an antioxidant	1196:1245	High DPPH scavenging ability was a positive indication to use EPS as an antioxidant.
26453848	4	28	theme	fermentation	586:597	arg1	process					599:605	the fermentation process	582:605	the fermentation process	582:605	Plackett-Burman statistical design was applied to evaluate the selected sixteen components from which, rice bran, peptone, NaCl and MnCl2 were found to be effective and significant on the fermentation process.
26453848	5	29	theme	following	741:749	arg1	concentrations					751:764	the following concentrations	737:764	the following concentrations	737:764	To study the concentration of each component, central composite design was carried out and response surface plots indicated that the following concentrations significantly enhanced the production - rice bran 5.02%, peptone 0.35%, NaCl 0.51%, MnCl2 0.074%.
26453848	7	30	theme	product	1005:1011	arg1	formation					1013:1021	product formation	1005:1021	product formation	1005:1021	Logistic model for microbial growth and Luedeking-Piret equation for product formation and substrate utilization were found to fit the experiment.
26453848	9	31	theme	scavenging	1173:1182	arg1	ability					1184:1190	High DPPH scavenging ability	1163:1190	High DPPH scavenging ability	1163:1190	High DPPH scavenging ability was a positive indication to use EPS as an antioxidant.
26453848	9	31	theme	scavenging	1173:1182	arg1	indication					1207:1216	a positive indication	1196:1216	a positive indication to use EPS as an antioxidant	1196:1245	High DPPH scavenging ability was a positive indication to use EPS as an antioxidant.
26453848	0	32	theme	antioxidant	27:37	arg1	activity					39:46	antioxidant activity	27:46	antioxidant activity	27:46	Optimization, kinetics and antioxidant activity of exopolysaccharide produced from rhizosphere isolate, Pseudomonas fluorescens CrN6.
26453848	3	33	theme	glucose	389:395	arg1	replacement					374:384	a replacement	372:384	a replacement of glucose	372:395	This study involved an agro waste as carbon substrate, rice bran, a replacement of glucose.
26453848	3	33	theme	glucose	389:395	arg1	substrate					350:358	carbon substrate	343:358	carbon substrate	343:358	This study involved an agro waste as carbon substrate, rice bran, a replacement of glucose.
26453848	7	34	theme	microbial	955:963	arg1	equation					992:999	microbial growth and Luedeking-Piret equation	955:999	microbial growth and Luedeking-Piret equation for product formation and substrate utilization	955:1047	Logistic model for microbial growth and Luedeking-Piret equation for product formation and substrate utilization were found to fit the experiment.
26453848	1	35	theme	exopolysaccharide	227:243	arg1	production					213:222	the production	209:222	the production of exopolysaccharide (EPS)	209:249	Pseudomonas fluorescens, isolated from rhizosphere soil, was exploited for the production of exopolysaccharide (EPS).
26453848	0	36	theme	exopolysaccharide	51:67	arg1	kinetics					14:21	kinetics	14:21	kinetics	14:21	Optimization, kinetics and antioxidant activity of exopolysaccharide produced from rhizosphere isolate, Pseudomonas fluorescens CrN6.
26453848	0	36	theme	exopolysaccharide	51:67	arg1	activity					39:46	antioxidant activity	27:46	antioxidant activity	27:46	Optimization, kinetics and antioxidant activity of exopolysaccharide produced from rhizosphere isolate, Pseudomonas fluorescens CrN6.
26453848	0	36	theme	exopolysaccharide	51:67	arg1	Optimization					0:11	Optimization	0:11	Optimization	0:11	Optimization, kinetics and antioxidant activity of exopolysaccharide produced from rhizosphere isolate, Pseudomonas fluorescens CrN6.
26453848	3	37	theme	carbon	343:348	arg1	bran					366:369	rice bran	361:369	rice bran	361:369	This study involved an agro waste as carbon substrate, rice bran, a replacement of glucose.
26453848	3	37	theme	carbon	343:348	arg1	replacement					374:384	a replacement	372:384	a replacement of glucose	372:395	This study involved an agro waste as carbon substrate, rice bran, a replacement of glucose.
26453848	3	37	theme	carbon	343:348	arg1	substrate					350:358	carbon substrate	343:358	carbon substrate	343:358	This study involved an agro waste as carbon substrate, rice bran, a replacement of glucose.
26453848	7	38	theme	growth	965:970	arg1	equation					992:999	microbial growth and Luedeking-Piret equation	955:999	microbial growth and Luedeking-Piret equation for product formation and substrate utilization	955:1047	Logistic model for microbial growth and Luedeking-Piret equation for product formation and substrate utilization were found to fit the experiment.
26453848	5	39	theme	MnCl2	850:854	arg1	%					820:820	rice bran 5.02%	806:820	rice bran 5.02%	806:820	To study the concentration of each component, central composite design was carried out and response surface plots indicated that the following concentrations significantly enhanced the production - rice bran 5.02%, peptone 0.35%, NaCl 0.51%, MnCl2 0.074%.
26453848	5	39	theme	MnCl2	850:854	arg1	%					861:861	MnCl2 0.074%	850:861	MnCl2 0.074%	850:861	To study the concentration of each component, central composite design was carried out and response surface plots indicated that the following concentrations significantly enhanced the production - rice bran 5.02%, peptone 0.35%, NaCl 0.51%, MnCl2 0.074%.
26453848	5	40	theme	component	643:651	arg1	concentration					621:633	the concentration	617:633	the concentration of each component	617:651	To study the concentration of each component, central composite design was carried out and response surface plots indicated that the following concentrations significantly enhanced the production - rice bran 5.02%, peptone 0.35%, NaCl 0.51%, MnCl2 0.074%.
26453848	4	41	theme	Plackett-Burman	398:412	arg1	design					426:431	Plackett-Burman statistical design	398:431	Plackett-Burman statistical design	398:431	Plackett-Burman statistical design was applied to evaluate the selected sixteen components from which, rice bran, peptone, NaCl and MnCl2 were found to be effective and significant on the fermentation process.
26453848	6	42	theme	Kinetic	864:870	arg1	modeling					872:879	Kinetic modeling	864:879	Kinetic modeling	864:879	Kinetic modeling was also performed to simulate the process parameters.
26453848	9	43	theme	positive	1198:1205	arg1	ability					1184:1190	High DPPH scavenging ability	1163:1190	High DPPH scavenging ability	1163:1190	High DPPH scavenging ability was a positive indication to use EPS as an antioxidant.
26453848	9	43	theme	positive	1198:1205	arg1	indication					1207:1216	a positive indication	1196:1216	a positive indication to use EPS as an antioxidant	1196:1245	High DPPH scavenging ability was a positive indication to use EPS as an antioxidant.
26453848	5	44	theme	central	654:660	arg1	design					672:677	central composite design	654:677	central composite design	654:677	To study the concentration of each component, central composite design was carried out and response surface plots indicated that the following concentrations significantly enhanced the production - rice bran 5.02%, peptone 0.35%, NaCl 0.51%, MnCl2 0.074%.
26453848	8	45	theme	present	1087:1093	arg1	investigation					1095:1107	The present investigation	1083:1107	The present investigation	1083:1107	The present investigation resulted in a maximum yield of 4.62g of EPS/L at 48h.
26453848	4	46	theme	statistical	414:424	arg1	design					426:431	Plackett-Burman statistical design	398:431	Plackett-Burman statistical design	398:431	Plackett-Burman statistical design was applied to evaluate the selected sixteen components from which, rice bran, peptone, NaCl and MnCl2 were found to be effective and significant on the fermentation process.
26453848	7	47	theme	Logistic	936:943	arg1	model					945:949	Logistic model	936:949	Logistic model for microbial growth and Luedeking-Piret equation for product formation and substrate utilization	936:1047	Logistic model for microbial growth and Luedeking-Piret equation for product formation and substrate utilization were found to fit the experiment.
26453848	5	48	theme	NaCl	838:841	arg1	%					847:847	NaCl 0.51%	838:847	NaCl 0.51%	838:847	To study the concentration of each component, central composite design was carried out and response surface plots indicated that the following concentrations significantly enhanced the production - rice bran 5.02%, peptone 0.35%, NaCl 0.51%, MnCl2 0.074%.
26453848	5	48	theme	NaCl	838:841	arg1	%					820:820	rice bran 5.02%	806:820	rice bran 5.02%	806:820	To study the concentration of each component, central composite design was carried out and response surface plots indicated that the following concentrations significantly enhanced the production - rice bran 5.02%, peptone 0.35%, NaCl 0.51%, MnCl2 0.074%.
26453848	3	49	theme	agro	329:332	arg1	waste					334:338	an agro waste	326:338	an agro waste	326:338	This study involved an agro waste as carbon substrate, rice bran, a replacement of glucose.
26453848	3	50	theme	rice	361:364	arg1	bran					366:369	rice bran	361:369	rice bran	361:369	This study involved an agro waste as carbon substrate, rice bran, a replacement of glucose.
26453848	3	50	theme	rice	361:364	arg1	substrate					350:358	carbon substrate	343:358	carbon substrate	343:358	This study involved an agro waste as carbon substrate, rice bran, a replacement of glucose.
28091940	2	0	from	groups	356:361	arg1	DS					406:407	DS	406:407	DS	406:407	Size, structural sequences and sulfate groups of oligosaccharides in the purified CS and DS could be characterized and identified using high performance liquid chromatography (HPLC) combined with Orbitrap mass spectrometry.
28091940	2	0	from	groups	356:361	arg1	CS					399:400	the purified CS	386:400	the purified CS	386:400	Size, structural sequences and sulfate groups of oligosaccharides in the purified CS and DS could be characterized and identified using high performance liquid chromatography (HPLC) combined with Orbitrap mass spectrometry.
28091940	4	1	theme	minor	792:796	arg1	differences					798:808	some minor differences	787:808	some minor differences of special structures	787:830	Structures of CS and DS oligosaccharides with different size and sulfate groups were compared between fishes and other animals, and results showed that some minor differences of special structures could be identified by hydrophilic interaction chromatography-liquid chromatography-fourier transform-mass/mass spectrometry (HILIC-LC-FT-MS/MS).
28091940	7	2	theme	different	1444:1452	arg1	origins					1454:1460	different origins	1444:1460	different origins	1444:1460	Understanding CS and DS structure derived from different origins may lead to the production of CS or DS with unique disaccharides or oligosaccharides sequence composition and biological functions.
28091940	6	3	theme	molecular	1331:1339	arg1	conformation					1341:1352	their molecular conformation	1325:1352	their molecular conformation	1325:1352	In addition, structural information of different origins of CS and DS was analyzed by principal component analysis (PCA) and results showed that CS and DS samples could be differentiated according to their molecular conformation and oligosaccharide fragments information.
28091940	6	4	theme	DS	1277:1278	arg1	samples					1280:1286	CS and DS samples	1270:1286	CS and DS samples	1270:1286	In addition, structural information of different origins of CS and DS was analyzed by principal component analysis (PCA) and results showed that CS and DS samples could be differentiated according to their molecular conformation and oligosaccharide fragments information.
28091940	5	5	theme	porcine	1113:1119	arg1	CS					1121:1122	porcine CS	1113:1122	porcine CS	1113:1122	For example, data showed that salmon and skipjack CS had a higher percentage content of high-level sulfated oligosaccharides than that porcine CS.
28091940	7	6	theme	unique	1506:1511	arg1	composition					1556:1566	unique disaccharides or oligosaccharides sequence composition	1506:1566	unique disaccharides or oligosaccharides sequence composition	1506:1566	Understanding CS and DS structure derived from different origins may lead to the production of CS or DS with unique disaccharides or oligosaccharides sequence composition and biological functions.
28091940	5	7	theme	higher	1037:1042	arg1	content					1055:1061	a higher percentage content	1035:1061	a higher percentage content of high-level sulfated oligosaccharides than that porcine CS	1035:1122	For example, data showed that salmon and skipjack CS had a higher percentage content of high-level sulfated oligosaccharides than that porcine CS.
28091940	2	8	theme	high	453:456	arg1	chromatography					477:490	high performance liquid chromatography	453:490	high performance liquid chromatography (HPLC) combined with Orbitrap mass spectrometry	453:538	Size, structural sequences and sulfate groups of oligosaccharides in the purified CS and DS could be characterized and identified using high performance liquid chromatography (HPLC) combined with Orbitrap mass spectrometry.
28091940	2	8	theme	high	453:456	arg1	HPLC					493:496	HPLC	493:496	HPLC	493:496	Size, structural sequences and sulfate groups of oligosaccharides in the purified CS and DS could be characterized and identified using high performance liquid chromatography (HPLC) combined with Orbitrap mass spectrometry.
28091940	1	9	theme	skipjack	281:288	arg1	tuna					290:293	skipjack tuna	281:293	skipjack tuna (Katsuwonus pelamis)	281:314	Chondroitin sulfate (CS) and dermatan sulfate (DS) were extracted and purified from skins or bones of salmon (Salmo salar), snakehead (Channa argus), monkfish (Lophius litulon) and skipjack tuna (Katsuwonus pelamis).
28091940	2	10	theme	mass	522:525	arg1	spectrometry					527:538	Orbitrap mass spectrometry	513:538	Orbitrap mass spectrometry	513:538	Size, structural sequences and sulfate groups of oligosaccharides in the purified CS and DS could be characterized and identified using high performance liquid chromatography (HPLC) combined with Orbitrap mass spectrometry.
28091940	1	11	dep	Channa	235:240	arg1	argus					242:246	Channa argus	235:246	Channa argus	235:246	Chondroitin sulfate (CS) and dermatan sulfate (DS) were extracted and purified from skins or bones of salmon (Salmo salar), snakehead (Channa argus), monkfish (Lophius litulon) and skipjack tuna (Katsuwonus pelamis).
28091940	6	12	theme	origins	1174:1180	arg1	information					1149:1159	structural information	1138:1159	structural information of different origins of CS and DS	1138:1193	In addition, structural information of different origins of CS and DS was analyzed by principal component analysis (PCA) and results showed that CS and DS samples could be differentiated according to their molecular conformation and oligosaccharide fragments information.
28091940	6	13	theme	principal	1211:1219	arg1	PCA					1241:1243	PCA	1241:1243	PCA	1241:1243	In addition, structural information of different origins of CS and DS was analyzed by principal component analysis (PCA) and results showed that CS and DS samples could be differentiated according to their molecular conformation and oligosaccharide fragments information.
28091940	6	13	theme	principal	1211:1219	arg1	analysis					1231:1238	principal component analysis	1211:1238	principal component analysis (PCA)	1211:1244	In addition, structural information of different origins of CS and DS was analyzed by principal component analysis (PCA) and results showed that CS and DS samples could be differentiated according to their molecular conformation and oligosaccharide fragments information.
28091940	4	14	theme	chromatography-liquid	879:899	arg1	HILIC-LC-FT-MS/MS					958:974	HILIC-LC-FT-MS/MS	958:974	HILIC-LC-FT-MS/MS	958:974	Structures of CS and DS oligosaccharides with different size and sulfate groups were compared between fishes and other animals, and results showed that some minor differences of special structures could be identified by hydrophilic interaction chromatography-liquid chromatography-fourier transform-mass/mass spectrometry (HILIC-LC-FT-MS/MS).
28091940	4	14	theme	chromatography-liquid	879:899	arg1	spectrometry					944:955	hydrophilic interaction chromatography-liquid chromatography-fourier transform-mass/mass spectrometry	855:955	hydrophilic interaction chromatography-liquid chromatography-fourier transform-mass/mass spectrometry (HILIC-LC-FT-MS/MS)	855:975	Structures of CS and DS oligosaccharides with different size and sulfate groups were compared between fishes and other animals, and results showed that some minor differences of special structures could be identified by hydrophilic interaction chromatography-liquid chromatography-fourier transform-mass/mass spectrometry (HILIC-LC-FT-MS/MS).
28091940	5	15	theme	high-level	1066:1075	arg1	oligosaccharides					1086:1101	high-level sulfated oligosaccharides	1066:1101	high-level sulfated oligosaccharides	1066:1101	For example, data showed that salmon and skipjack CS had a higher percentage content of high-level sulfated oligosaccharides than that porcine CS.
28091940	2	16	theme	Orbitrap	513:520	arg1	spectrometry					527:538	Orbitrap mass spectrometry	513:538	Orbitrap mass spectrometry	513:538	Size, structural sequences and sulfate groups of oligosaccharides in the purified CS and DS could be characterized and identified using high performance liquid chromatography (HPLC) combined with Orbitrap mass spectrometry.
28091940	6	17	theme	different	1164:1172	arg1	origins					1174:1180	different origins	1164:1180	different origins of CS and DS	1164:1193	In addition, structural information of different origins of CS and DS was analyzed by principal component analysis (PCA) and results showed that CS and DS samples could be differentiated according to their molecular conformation and oligosaccharide fragments information.
28091940	4	18	theme	CS	649:650	arg1	oligosaccharides					659:674	CS and DS oligosaccharides	649:674	CS and DS oligosaccharides with different size and sulfate groups	649:713	Structures of CS and DS oligosaccharides with different size and sulfate groups were compared between fishes and other animals, and results showed that some minor differences of special structures could be identified by hydrophilic interaction chromatography-liquid chromatography-fourier transform-mass/mass spectrometry (HILIC-LC-FT-MS/MS).
28091940	5	19	theme	sulfated	1077:1084	arg1	oligosaccharides					1086:1101	high-level sulfated oligosaccharides	1066:1101	high-level sulfated oligosaccharides	1066:1101	For example, data showed that salmon and skipjack CS had a higher percentage content of high-level sulfated oligosaccharides than that porcine CS.
28091940	7	20	with	CS	1492:1493	arg1	composition					1556:1566	unique disaccharides or oligosaccharides sequence composition	1506:1566	unique disaccharides or oligosaccharides sequence composition	1506:1566	Understanding CS and DS structure derived from different origins may lead to the production of CS or DS with unique disaccharides or oligosaccharides sequence composition and biological functions.
28091940	7	20	with	CS	1492:1493	arg1	functions					1583:1591	biological functions	1572:1591	biological functions	1572:1591	Understanding CS and DS structure derived from different origins may lead to the production of CS or DS with unique disaccharides or oligosaccharides sequence composition and biological functions.
28091940	4	21	theme	transform-mass/mass	924:942	arg1	HILIC-LC-FT-MS/MS					958:974	HILIC-LC-FT-MS/MS	958:974	HILIC-LC-FT-MS/MS	958:974	Structures of CS and DS oligosaccharides with different size and sulfate groups were compared between fishes and other animals, and results showed that some minor differences of special structures could be identified by hydrophilic interaction chromatography-liquid chromatography-fourier transform-mass/mass spectrometry (HILIC-LC-FT-MS/MS).
28091940	4	21	theme	transform-mass/mass	924:942	arg1	spectrometry					944:955	hydrophilic interaction chromatography-liquid chromatography-fourier transform-mass/mass spectrometry	855:955	hydrophilic interaction chromatography-liquid chromatography-fourier transform-mass/mass spectrometry (HILIC-LC-FT-MS/MS)	855:975	Structures of CS and DS oligosaccharides with different size and sulfate groups were compared between fishes and other animals, and results showed that some minor differences of special structures could be identified by hydrophilic interaction chromatography-liquid chromatography-fourier transform-mass/mass spectrometry (HILIC-LC-FT-MS/MS).
28091940	5	22	theme	oligosaccharides	1086:1101	arg1	content					1055:1061	a higher percentage content	1035:1061	a higher percentage content of high-level sulfated oligosaccharides than that porcine CS	1035:1122	For example, data showed that salmon and skipjack CS had a higher percentage content of high-level sulfated oligosaccharides than that porcine CS.
28091940	6	23	dep	conformation	1341:1352	arg1	information					1384:1394	information	1384:1394	information	1384:1394	In addition, structural information of different origins of CS and DS was analyzed by principal component analysis (PCA) and results showed that CS and DS samples could be differentiated according to their molecular conformation and oligosaccharide fragments information.
28091940	4	24	theme	sulfate	700:706	arg1	groups					708:713	different size and sulfate groups	681:713	groups	708:713	Structures of CS and DS oligosaccharides with different size and sulfate groups were compared between fishes and other animals, and results showed that some minor differences of special structures could be identified by hydrophilic interaction chromatography-liquid chromatography-fourier transform-mass/mass spectrometry (HILIC-LC-FT-MS/MS).
28091940	4	25	theme	interaction	867:877	arg1	HILIC-LC-FT-MS/MS					958:974	HILIC-LC-FT-MS/MS	958:974	HILIC-LC-FT-MS/MS	958:974	Structures of CS and DS oligosaccharides with different size and sulfate groups were compared between fishes and other animals, and results showed that some minor differences of special structures could be identified by hydrophilic interaction chromatography-liquid chromatography-fourier transform-mass/mass spectrometry (HILIC-LC-FT-MS/MS).
28091940	4	25	theme	interaction	867:877	arg1	spectrometry					944:955	hydrophilic interaction chromatography-liquid chromatography-fourier transform-mass/mass spectrometry	855:955	hydrophilic interaction chromatography-liquid chromatography-fourier transform-mass/mass spectrometry (HILIC-LC-FT-MS/MS)	855:975	Structures of CS and DS oligosaccharides with different size and sulfate groups were compared between fishes and other animals, and results showed that some minor differences of special structures could be identified by hydrophilic interaction chromatography-liquid chromatography-fourier transform-mass/mass spectrometry (HILIC-LC-FT-MS/MS).
28091940	4	26	with	oligosaccharides	659:674	arg1	size					691:694	different size and sulfate groups	681:713	size	691:694	Structures of CS and DS oligosaccharides with different size and sulfate groups were compared between fishes and other animals, and results showed that some minor differences of special structures could be identified by hydrophilic interaction chromatography-liquid chromatography-fourier transform-mass/mass spectrometry (HILIC-LC-FT-MS/MS).
28091940	4	26	with	oligosaccharides	659:674	arg1	groups					708:713	different size and sulfate groups	681:713	groups	708:713	Structures of CS and DS oligosaccharides with different size and sulfate groups were compared between fishes and other animals, and results showed that some minor differences of special structures could be identified by hydrophilic interaction chromatography-liquid chromatography-fourier transform-mass/mass spectrometry (HILIC-LC-FT-MS/MS).
28091940	6	27	theme	structural	1138:1147	arg1	information					1149:1159	structural information	1138:1159	structural information of different origins of CS and DS	1138:1193	In addition, structural information of different origins of CS and DS was analyzed by principal component analysis (PCA) and results showed that CS and DS samples could be differentiated according to their molecular conformation and oligosaccharide fragments information.
28091940	4	28	theme	oligosaccharides	659:674	arg1	Structures					635:644	Structures	635:644	Structures of CS and DS oligosaccharides with different size and sulfate groups	635:713	Structures of CS and DS oligosaccharides with different size and sulfate groups were compared between fishes and other animals, and results showed that some minor differences of special structures could be identified by hydrophilic interaction chromatography-liquid chromatography-fourier transform-mass/mass spectrometry (HILIC-LC-FT-MS/MS).
28091940	7	29	with	DS	1498:1499	arg1	composition					1556:1566	unique disaccharides or oligosaccharides sequence composition	1506:1566	unique disaccharides or oligosaccharides sequence composition	1506:1566	Understanding CS and DS structure derived from different origins may lead to the production of CS or DS with unique disaccharides or oligosaccharides sequence composition and biological functions.
28091940	7	29	with	DS	1498:1499	arg1	functions					1583:1591	biological functions	1572:1591	biological functions	1572:1591	Understanding CS and DS structure derived from different origins may lead to the production of CS or DS with unique disaccharides or oligosaccharides sequence composition and biological functions.
28091940	2	30	theme	oligosaccharides	366:381	arg1	Size					317:320	Size	317:320	Size	317:320	Size, structural sequences and sulfate groups of oligosaccharides in the purified CS and DS could be characterized and identified using high performance liquid chromatography (HPLC) combined with Orbitrap mass spectrometry.
28091940	2	30	theme	oligosaccharides	366:381	arg1	sequences					334:342	structural sequences	323:342	structural sequences	323:342	Size, structural sequences and sulfate groups of oligosaccharides in the purified CS and DS could be characterized and identified using high performance liquid chromatography (HPLC) combined with Orbitrap mass spectrometry.
28091940	2	30	theme	oligosaccharides	366:381	arg1	groups					356:361	sulfate groups	348:361	sulfate groups	348:361	Size, structural sequences and sulfate groups of oligosaccharides in the purified CS and DS could be characterized and identified using high performance liquid chromatography (HPLC) combined with Orbitrap mass spectrometry.
28091940	2	30	theme	oligosaccharides	366:381	arg1	oligosaccharides					366:381	oligosaccharides	366:381	oligosaccharides	366:381	Size, structural sequences and sulfate groups of oligosaccharides in the purified CS and DS could be characterized and identified using high performance liquid chromatography (HPLC) combined with Orbitrap mass spectrometry.
28091940	6	31	theme	CS	1185:1186	arg1	origins					1174:1180	different origins	1164:1180	different origins of CS and DS	1164:1193	In addition, structural information of different origins of CS and DS was analyzed by principal component analysis (PCA) and results showed that CS and DS samples could be differentiated according to their molecular conformation and oligosaccharide fragments information.
28091940	6	32	theme	component	1221:1229	arg1	PCA					1241:1243	PCA	1241:1243	PCA	1241:1243	In addition, structural information of different origins of CS and DS was analyzed by principal component analysis (PCA) and results showed that CS and DS samples could be differentiated according to their molecular conformation and oligosaccharide fragments information.
28091940	6	32	theme	component	1221:1229	arg1	analysis					1231:1238	principal component analysis	1211:1238	principal component analysis (PCA)	1211:1244	In addition, structural information of different origins of CS and DS was analyzed by principal component analysis (PCA) and results showed that CS and DS samples could be differentiated according to their molecular conformation and oligosaccharide fragments information.
28091940	1	33	theme	Chondroitin	100:110	arg1	CS					121:122	CS	121:122	CS	121:122	Chondroitin sulfate (CS) and dermatan sulfate (DS) were extracted and purified from skins or bones of salmon (Salmo salar), snakehead (Channa argus), monkfish (Lophius litulon) and skipjack tuna (Katsuwonus pelamis).
28091940	1	33	theme	Chondroitin	100:110	arg1	sulfate					112:118	Chondroitin sulfate	100:118	Chondroitin sulfate (CS)	100:123	Chondroitin sulfate (CS) and dermatan sulfate (DS) were extracted and purified from skins or bones of salmon (Salmo salar), snakehead (Channa argus), monkfish (Lophius litulon) and skipjack tuna (Katsuwonus pelamis).
28091940	4	34	theme	DS	656:657	arg1	oligosaccharides					659:674	CS and DS oligosaccharides	649:674	CS and DS oligosaccharides with different size and sulfate groups	649:713	Structures of CS and DS oligosaccharides with different size and sulfate groups were compared between fishes and other animals, and results showed that some minor differences of special structures could be identified by hydrophilic interaction chromatography-liquid chromatography-fourier transform-mass/mass spectrometry (HILIC-LC-FT-MS/MS).
28091940	7	35	theme	sequence	1547:1554	arg1	composition					1556:1566	unique disaccharides or oligosaccharides sequence composition	1506:1566	unique disaccharides or oligosaccharides sequence composition	1506:1566	Understanding CS and DS structure derived from different origins may lead to the production of CS or DS with unique disaccharides or oligosaccharides sequence composition and biological functions.
28091940	4	36	theme	different	681:689	arg1	size					691:694	different size and sulfate groups	681:713	size	691:694	Structures of CS and DS oligosaccharides with different size and sulfate groups were compared between fishes and other animals, and results showed that some minor differences of special structures could be identified by hydrophilic interaction chromatography-liquid chromatography-fourier transform-mass/mass spectrometry (HILIC-LC-FT-MS/MS).
28091940	4	37	theme	other	748:752	arg1	animals					754:760	other animals	748:760	other animals	748:760	Structures of CS and DS oligosaccharides with different size and sulfate groups were compared between fishes and other animals, and results showed that some minor differences of special structures could be identified by hydrophilic interaction chromatography-liquid chromatography-fourier transform-mass/mass spectrometry (HILIC-LC-FT-MS/MS).
28091940	2	38	theme	sulfate	348:354	arg1	oligosaccharides					366:381	oligosaccharides	366:381	oligosaccharides	366:381	Size, structural sequences and sulfate groups of oligosaccharides in the purified CS and DS could be characterized and identified using high performance liquid chromatography (HPLC) combined with Orbitrap mass spectrometry.
28091940	2	38	theme	sulfate	348:354	arg1	groups					356:361	sulfate groups	348:361	sulfate groups	348:361	Size, structural sequences and sulfate groups of oligosaccharides in the purified CS and DS could be characterized and identified using high performance liquid chromatography (HPLC) combined with Orbitrap mass spectrometry.
28091940	0	39	theme	sequence	17:24	arg1	characterization					26:41	sequence characterization	17:41	sequence characterization	17:41	Purification and sequence characterization of chondroitin sulfate and dermatan sulfate from fishes.
28091940	0	40	from	fishes	92:97	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification and sequence characterization of chondroitin sulfate and dermatan sulfate from fishes.
28091940	0	40	from	fishes	92:97	arg1	characterization					26:41	sequence characterization	17:41	sequence characterization	17:41	Purification and sequence characterization of chondroitin sulfate and dermatan sulfate from fishes.
28091940	7	41	dep	CS	1411:1412	arg1	structure					1421:1429	structure	1421:1429	structure	1421:1429	Understanding CS and DS structure derived from different origins may lead to the production of CS or DS with unique disaccharides or oligosaccharides sequence composition and biological functions.
28091940	0	42	theme	chondroitin	46:56	arg1	sulfate					58:64	chondroitin sulfate	46:64	chondroitin sulfate	46:64	Purification and sequence characterization of chondroitin sulfate and dermatan sulfate from fishes.
28091940	2	43	theme	liquid	470:475	arg1	chromatography					477:490	high performance liquid chromatography	453:490	high performance liquid chromatography (HPLC) combined with Orbitrap mass spectrometry	453:538	Size, structural sequences and sulfate groups of oligosaccharides in the purified CS and DS could be characterized and identified using high performance liquid chromatography (HPLC) combined with Orbitrap mass spectrometry.
28091940	2	43	theme	liquid	470:475	arg1	HPLC					493:496	HPLC	493:496	HPLC	493:496	Size, structural sequences and sulfate groups of oligosaccharides in the purified CS and DS could be characterized and identified using high performance liquid chromatography (HPLC) combined with Orbitrap mass spectrometry.
28091940	5	44	theme	percentage	1044:1053	arg1	content					1055:1061	a higher percentage content	1035:1061	a higher percentage content of high-level sulfated oligosaccharides than that porcine CS	1035:1122	For example, data showed that salmon and skipjack CS had a higher percentage content of high-level sulfated oligosaccharides than that porcine CS.
28091940	4	45	theme	hydrophilic	855:865	arg1	HILIC-LC-FT-MS/MS					958:974	HILIC-LC-FT-MS/MS	958:974	HILIC-LC-FT-MS/MS	958:974	Structures of CS and DS oligosaccharides with different size and sulfate groups were compared between fishes and other animals, and results showed that some minor differences of special structures could be identified by hydrophilic interaction chromatography-liquid chromatography-fourier transform-mass/mass spectrometry (HILIC-LC-FT-MS/MS).
28091940	4	45	theme	hydrophilic	855:865	arg1	spectrometry					944:955	hydrophilic interaction chromatography-liquid chromatography-fourier transform-mass/mass spectrometry	855:955	hydrophilic interaction chromatography-liquid chromatography-fourier transform-mass/mass spectrometry (HILIC-LC-FT-MS/MS)	855:975	Structures of CS and DS oligosaccharides with different size and sulfate groups were compared between fishes and other animals, and results showed that some minor differences of special structures could be identified by hydrophilic interaction chromatography-liquid chromatography-fourier transform-mass/mass spectrometry (HILIC-LC-FT-MS/MS).
28091940	5	46	contain	had	1031:1033	arg2	content					1055:1061	a higher percentage content	1035:1061	a higher percentage content of high-level sulfated oligosaccharides than that porcine CS	1035:1122	For example, data showed that salmon and skipjack CS had a higher percentage content of high-level sulfated oligosaccharides than that porcine CS.
28091940	5	46	contain	had	1031:1033	arg1	salmon					1008:1013	salmon	1008:1013	salmon	1008:1013	For example, data showed that salmon and skipjack CS had a higher percentage content of high-level sulfated oligosaccharides than that porcine CS.
28091940	5	46	contain	had	1031:1033	arg1	CS					1028:1029	skipjack CS	1019:1029	skipjack CS	1019:1029	For example, data showed that salmon and skipjack CS had a higher percentage content of high-level sulfated oligosaccharides than that porcine CS.
28091940	7	47	attach	derived	1431:1437	arg1	origins					1454:1460	different origins	1444:1460	different origins	1444:1460	Understanding CS and DS structure derived from different origins may lead to the production of CS or DS with unique disaccharides or oligosaccharides sequence composition and biological functions.
28091940	7	47	attach	derived	1431:1437	arg2	CS					1411:1412	CS	1411:1412	CS	1411:1412	Understanding CS and DS structure derived from different origins may lead to the production of CS or DS with unique disaccharides or oligosaccharides sequence composition and biological functions.
28091940	2	48	theme	performance	458:468	arg1	chromatography					477:490	high performance liquid chromatography	453:490	high performance liquid chromatography (HPLC) combined with Orbitrap mass spectrometry	453:538	Size, structural sequences and sulfate groups of oligosaccharides in the purified CS and DS could be characterized and identified using high performance liquid chromatography (HPLC) combined with Orbitrap mass spectrometry.
28091940	2	48	theme	performance	458:468	arg1	HPLC					493:496	HPLC	493:496	HPLC	493:496	Size, structural sequences and sulfate groups of oligosaccharides in the purified CS and DS could be characterized and identified using high performance liquid chromatography (HPLC) combined with Orbitrap mass spectrometry.
28091940	2	49	theme	structural	323:332	arg1	sequences					334:342	structural sequences	323:342	structural sequences	323:342	Size, structural sequences and sulfate groups of oligosaccharides in the purified CS and DS could be characterized and identified using high performance liquid chromatography (HPLC) combined with Orbitrap mass spectrometry.
28091940	2	50	theme	purified	390:397	arg1	CS					399:400	the purified CS	386:400	the purified CS	386:400	Size, structural sequences and sulfate groups of oligosaccharides in the purified CS and DS could be characterized and identified using high performance liquid chromatography (HPLC) combined with Orbitrap mass spectrometry.
28091940	4	51	theme	chromatography-fourier	901:922	arg1	HILIC-LC-FT-MS/MS					958:974	HILIC-LC-FT-MS/MS	958:974	HILIC-LC-FT-MS/MS	958:974	Structures of CS and DS oligosaccharides with different size and sulfate groups were compared between fishes and other animals, and results showed that some minor differences of special structures could be identified by hydrophilic interaction chromatography-liquid chromatography-fourier transform-mass/mass spectrometry (HILIC-LC-FT-MS/MS).
28091940	4	51	theme	chromatography-fourier	901:922	arg1	spectrometry					944:955	hydrophilic interaction chromatography-liquid chromatography-fourier transform-mass/mass spectrometry	855:955	hydrophilic interaction chromatography-liquid chromatography-fourier transform-mass/mass spectrometry (HILIC-LC-FT-MS/MS)	855:975	Structures of CS and DS oligosaccharides with different size and sulfate groups were compared between fishes and other animals, and results showed that some minor differences of special structures could be identified by hydrophilic interaction chromatography-liquid chromatography-fourier transform-mass/mass spectrometry (HILIC-LC-FT-MS/MS).
28091940	4	52	theme	structures	821:830	arg1	differences					798:808	some minor differences	787:808	some minor differences of special structures	787:830	Structures of CS and DS oligosaccharides with different size and sulfate groups were compared between fishes and other animals, and results showed that some minor differences of special structures could be identified by hydrophilic interaction chromatography-liquid chromatography-fourier transform-mass/mass spectrometry (HILIC-LC-FT-MS/MS).
28091940	6	53	theme	oligosaccharide	1358:1372	arg1	fragments					1374:1382	oligosaccharide fragments	1358:1382	oligosaccharide fragments	1358:1382	In addition, structural information of different origins of CS and DS was analyzed by principal component analysis (PCA) and results showed that CS and DS samples could be differentiated according to their molecular conformation and oligosaccharide fragments information.
28091940	7	54	theme	oligosaccharides	1530:1545	arg1	composition					1556:1566	unique disaccharides or oligosaccharides sequence composition	1506:1566	unique disaccharides or oligosaccharides sequence composition	1506:1566	Understanding CS and DS structure derived from different origins may lead to the production of CS or DS with unique disaccharides or oligosaccharides sequence composition and biological functions.
28091940	0	55	theme	sulfate	58:64	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification and sequence characterization of chondroitin sulfate and dermatan sulfate from fishes.
28091940	0	55	theme	sulfate	58:64	arg1	characterization					26:41	sequence characterization	17:41	sequence characterization	17:41	Purification and sequence characterization of chondroitin sulfate and dermatan sulfate from fishes.
28091940	1	56	dep	tuna	290:293	arg1	pelamis					307:313	Katsuwonus pelamis	296:313	Katsuwonus pelamis	296:313	Chondroitin sulfate (CS) and dermatan sulfate (DS) were extracted and purified from skins or bones of salmon (Salmo salar), snakehead (Channa argus), monkfish (Lophius litulon) and skipjack tuna (Katsuwonus pelamis).
28091940	4	57	theme	special	813:819	arg1	structures					821:830	special structures	813:830	special structures	813:830	Structures of CS and DS oligosaccharides with different size and sulfate groups were compared between fishes and other animals, and results showed that some minor differences of special structures could be identified by hydrophilic interaction chromatography-liquid chromatography-fourier transform-mass/mass spectrometry (HILIC-LC-FT-MS/MS).
28091940	5	58	theme	skipjack	1019:1026	arg1	CS					1028:1029	skipjack CS	1019:1029	skipjack CS	1019:1029	For example, data showed that salmon and skipjack CS had a higher percentage content of high-level sulfated oligosaccharides than that porcine CS.
28091940	7	59	theme	DS	1498:1499	arg1	production					1478:1487	the production	1474:1487	the production of CS or DS with unique disaccharides or oligosaccharides sequence composition and biological functions	1474:1591	Understanding CS and DS structure derived from different origins may lead to the production of CS or DS with unique disaccharides or oligosaccharides sequence composition and biological functions.
28091940	1	60	theme	Lophius	260:266	arg1	monkfish					250:257	monkfish	250:257	monkfish (Lophius litulon)	250:275	Chondroitin sulfate (CS) and dermatan sulfate (DS) were extracted and purified from skins or bones of salmon (Salmo salar), snakehead (Channa argus), monkfish (Lophius litulon) and skipjack tuna (Katsuwonus pelamis).
28091940	1	60	theme	Lophius	260:266	arg1	litulon					268:274	Lophius litulon	260:274	Lophius litulon	260:274	Chondroitin sulfate (CS) and dermatan sulfate (DS) were extracted and purified from skins or bones of salmon (Salmo salar), snakehead (Channa argus), monkfish (Lophius litulon) and skipjack tuna (Katsuwonus pelamis).
28091940	6	61	theme	CS	1270:1271	arg1	samples					1280:1286	CS and DS samples	1270:1286	CS and DS samples	1270:1286	In addition, structural information of different origins of CS and DS was analyzed by principal component analysis (PCA) and results showed that CS and DS samples could be differentiated according to their molecular conformation and oligosaccharide fragments information.
28091940	3	62	theme	motif	599:603	arg1	structure					605:613	motif structure	599:613	motif structure	599:613	CS and DS chain structure varies depending on origin, but motif structure appears consistent.
28091940	3	63	theme	DS	548:549	arg1	structure					557:565	CS and DS chain structure	541:565	CS and DS chain structure	541:565	CS and DS chain structure varies depending on origin, but motif structure appears consistent.
28091940	2	64	from	Size	317:320	arg1	DS					406:407	DS	406:407	DS	406:407	Size, structural sequences and sulfate groups of oligosaccharides in the purified CS and DS could be characterized and identified using high performance liquid chromatography (HPLC) combined with Orbitrap mass spectrometry.
28091940	2	64	from	Size	317:320	arg1	CS					399:400	the purified CS	386:400	the purified CS	386:400	Size, structural sequences and sulfate groups of oligosaccharides in the purified CS and DS could be characterized and identified using high performance liquid chromatography (HPLC) combined with Orbitrap mass spectrometry.
28091940	3	65	theme	chain	551:555	arg1	structure					557:565	CS and DS chain structure	541:565	CS and DS chain structure	541:565	CS and DS chain structure varies depending on origin, but motif structure appears consistent.
28091940	2	66	from	sequences	334:342	arg1	DS					406:407	DS	406:407	DS	406:407	Size, structural sequences and sulfate groups of oligosaccharides in the purified CS and DS could be characterized and identified using high performance liquid chromatography (HPLC) combined with Orbitrap mass spectrometry.
28091940	2	66	from	sequences	334:342	arg1	CS					399:400	the purified CS	386:400	the purified CS	386:400	Size, structural sequences and sulfate groups of oligosaccharides in the purified CS and DS could be characterized and identified using high performance liquid chromatography (HPLC) combined with Orbitrap mass spectrometry.
28091940	1	67	theme	dermatan	129:136	arg1	DS					147:148	DS	147:148	DS	147:148	Chondroitin sulfate (CS) and dermatan sulfate (DS) were extracted and purified from skins or bones of salmon (Salmo salar), snakehead (Channa argus), monkfish (Lophius litulon) and skipjack tuna (Katsuwonus pelamis).
28091940	1	67	theme	dermatan	129:136	arg1	sulfate					138:144	dermatan sulfate	129:144	dermatan sulfate (DS)	129:149	Chondroitin sulfate (CS) and dermatan sulfate (DS) were extracted and purified from skins or bones of salmon (Salmo salar), snakehead (Channa argus), monkfish (Lophius litulon) and skipjack tuna (Katsuwonus pelamis).
28091940	1	68	theme	salmon	202:207	arg1	monkfish					250:257	monkfish	250:257	monkfish (Lophius litulon)	250:275	Chondroitin sulfate (CS) and dermatan sulfate (DS) were extracted and purified from skins or bones of salmon (Salmo salar), snakehead (Channa argus), monkfish (Lophius litulon) and skipjack tuna (Katsuwonus pelamis).
28091940	1	68	theme	salmon	202:207	arg1	tuna					290:293	skipjack tuna	281:293	skipjack tuna (Katsuwonus pelamis)	281:314	Chondroitin sulfate (CS) and dermatan sulfate (DS) were extracted and purified from skins or bones of salmon (Salmo salar), snakehead (Channa argus), monkfish (Lophius litulon) and skipjack tuna (Katsuwonus pelamis).
28091940	1	68	theme	salmon	202:207	arg1	Channa					235:240	Channa	235:240	Channa	235:240	Chondroitin sulfate (CS) and dermatan sulfate (DS) were extracted and purified from skins or bones of salmon (Salmo salar), snakehead (Channa argus), monkfish (Lophius litulon) and skipjack tuna (Katsuwonus pelamis).
28091940	1	68	theme	salmon	202:207	arg1	skins					184:188	skins	184:188	skins	184:188	Chondroitin sulfate (CS) and dermatan sulfate (DS) were extracted and purified from skins or bones of salmon (Salmo salar), snakehead (Channa argus), monkfish (Lophius litulon) and skipjack tuna (Katsuwonus pelamis).
28091940	1	68	theme	salmon	202:207	arg1	litulon					268:274	Lophius litulon	260:274	Lophius litulon	260:274	Chondroitin sulfate (CS) and dermatan sulfate (DS) were extracted and purified from skins or bones of salmon (Salmo salar), snakehead (Channa argus), monkfish (Lophius litulon) and skipjack tuna (Katsuwonus pelamis).
28091940	1	68	theme	salmon	202:207	arg1	salar					216:220	salar	216:220	salar	216:220	Chondroitin sulfate (CS) and dermatan sulfate (DS) were extracted and purified from skins or bones of salmon (Salmo salar), snakehead (Channa argus), monkfish (Lophius litulon) and skipjack tuna (Katsuwonus pelamis).
28091940	1	68	theme	salmon	202:207	arg1	bones					193:197	bones	193:197	bones of salmon	193:207	Chondroitin sulfate (CS) and dermatan sulfate (DS) were extracted and purified from skins or bones of salmon (Salmo salar), snakehead (Channa argus), monkfish (Lophius litulon) and skipjack tuna (Katsuwonus pelamis).
28091940	1	68	theme	salmon	202:207	arg1	snakehead					224:232	snakehead	224:232	snakehead (Channa argus)	224:247	Chondroitin sulfate (CS) and dermatan sulfate (DS) were extracted and purified from skins or bones of salmon (Salmo salar), snakehead (Channa argus), monkfish (Lophius litulon) and skipjack tuna (Katsuwonus pelamis).
28091940	0	69	theme	sulfate	79:85	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification and sequence characterization of chondroitin sulfate and dermatan sulfate from fishes.
28091940	0	69	theme	sulfate	79:85	arg1	characterization					26:41	sequence characterization	17:41	sequence characterization	17:41	Purification and sequence characterization of chondroitin sulfate and dermatan sulfate from fishes.
28091940	7	70	theme	CS	1492:1493	arg1	production					1478:1487	the production	1474:1487	the production of CS or DS with unique disaccharides or oligosaccharides sequence composition and biological functions	1474:1591	Understanding CS and DS structure derived from different origins may lead to the production of CS or DS with unique disaccharides or oligosaccharides sequence composition and biological functions.
28091940	3	71	theme	CS	541:542	arg1	structure					557:565	CS and DS chain structure	541:565	CS and DS chain structure	541:565	CS and DS chain structure varies depending on origin, but motif structure appears consistent.
28091940	7	72	theme	biological	1572:1581	arg1	functions					1583:1591	biological functions	1572:1591	biological functions	1572:1591	Understanding CS and DS structure derived from different origins may lead to the production of CS or DS with unique disaccharides or oligosaccharides sequence composition and biological functions.
28091940	0	73	theme	dermatan	70:77	arg1	sulfate					79:85	dermatan sulfate	70:85	dermatan sulfate	70:85	Purification and sequence characterization of chondroitin sulfate and dermatan sulfate from fishes.
28091940	6	74	theme	DS	1192:1193	arg1	origins					1174:1180	different origins	1164:1180	different origins of CS and DS	1164:1193	In addition, structural information of different origins of CS and DS was analyzed by principal component analysis (PCA) and results showed that CS and DS samples could be differentiated according to their molecular conformation and oligosaccharide fragments information.
28091940	7	75	theme	disaccharides	1513:1525	arg1	composition					1556:1566	unique disaccharides or oligosaccharides sequence composition	1506:1566	unique disaccharides or oligosaccharides sequence composition	1506:1566	Understanding CS and DS structure derived from different origins may lead to the production of CS or DS with unique disaccharides or oligosaccharides sequence composition and biological functions.
29209879	5	0	theme	sGAG	738:741	arg1	estimation					724:733	estimation	724:733	estimation of sGAG by DMMB dye-binding assay	724:767	sGAG-DMMB complex formed after estimation of sGAG by DMMB dye-binding assay was decomplexed and sGAGs were recovered.
29209879	9	1	theme	recovered	1356:1364	arg1	sGAGs					1366:1370	recovered sGAGs	1356:1370	recovered sGAGs	1356:1370	Analysis of recovered sGAGs, specifically heparan sulfate by HPLC showed characteristic disaccharide composition akin to that of GAG obtained by the conventional protocol.
29209879	9	1	theme	recovered	1356:1364	arg1	sulfate					1394:1400	heparan sulfate	1386:1400	specifically heparan sulfate	1373:1400	Analysis of recovered sGAGs, specifically heparan sulfate by HPLC showed characteristic disaccharide composition akin to that of GAG obtained by the conventional protocol.
29209879	2	2	theme	biological	339:348	arg1	studies					350:356	various structural, functional and biological studies	304:356	various structural, functional and biological studies	304:356	This is compounded by the fact that the analysis is technique-intensive and more often there will be a limitation on the quantity of GAGs available for various structural, functional and biological studies.
29209879	10	3	theme	present	1529:1535	arg1	paper					1537:1541	the present paper	1525:1541	the present paper	1525:1541	Thus, in the present paper, we show that sGAG can be recovered in comparatively purer form after routine estimation and can be used for further analysis thus saving up on the precious sample.
29209879	3	4	theme	composition	489:499	arg1	estimation					422:431	estimation	422:431	estimation	422:431	In such a scenario, the sample which can be made available for estimation and elucidation of disaccharide composition and species composition as well remains a challenge.
29209879	3	4	theme	composition	489:499	arg1	elucidation					437:447	elucidation	437:447	elucidation	437:447	In such a scenario, the sample which can be made available for estimation and elucidation of disaccharide composition and species composition as well remains a challenge.
29209879	5	5	theme	sGAG-DMMB	693:701	arg1	complex					703:709	sGAG-DMMB complex	693:709	sGAG-DMMB complex formed after estimation of sGAG by DMMB dye-binding assay	693:767	sGAG-DMMB complex formed after estimation of sGAG by DMMB dye-binding assay was decomplexed and sGAGs were recovered.
29209879	8	6	theme	good	1165:1168	arg1	separation					1170:1179	good separation	1165:1179	good separation between species of sGAGs namely chondroitin/dermatan sulfate and heparan sulfate	1165:1260	Further analysis using cellulose acetate membrane electrophoresis showed good separation between species of sGAGs namely chondroitin/dermatan sulfate and heparan sulfate, with comparatively lesser interference from hyaluronic acid, a non-sulfated GAG.
29209879	10	7	theme	further	1652:1658	arg1	analysis					1660:1667	further analysis	1652:1667	further analysis thus saving up on the precious sample	1652:1705	Thus, in the present paper, we show that sGAG can be recovered in comparatively purer form after routine estimation and can be used for further analysis thus saving up on the precious sample.
29209879	3	8	theme	species	481:487	arg1	composition					489:499	species composition	481:499	species composition	481:499	In such a scenario, the sample which can be made available for estimation and elucidation of disaccharide composition and species composition as well remains a challenge.
29209879	3	9	theme	disaccharide	452:463	arg1	composition					465:475	disaccharide composition	452:475	disaccharide composition	452:475	In such a scenario, the sample which can be made available for estimation and elucidation of disaccharide composition and species composition as well remains a challenge.
29209879	2	10	theme	functional	324:333	arg1	studies					350:356	various structural, functional and biological studies	304:356	various structural, functional and biological studies	304:356	This is compounded by the fact that the analysis is technique-intensive and more often there will be a limitation on the quantity of GAGs available for various structural, functional and biological studies.
29209879	4	11	theme	sulfated	602:609	arg1	sGAG					617:620	sGAG	617:620	sGAG	617:620	In the present study, we have determined the feasibility where isolated sulfated GAGs (sGAG) that is estimated by metachromasia is recovered for further analysis.
29209879	4	11	theme	sulfated	602:609	arg1	GAGs					611:614	isolated sulfated GAGs	593:614	isolated sulfated GAGs (sGAG) that is estimated by metachromasia	593:656	In the present study, we have determined the feasibility where isolated sulfated GAGs (sGAG) that is estimated by metachromasia is recovered for further analysis.
29209879	8	12	theme	heparan	1246:1252	arg1	sGAGs					1200:1204	sGAGs	1200:1204	sGAGs namely chondroitin/dermatan sulfate and heparan sulfate	1200:1260	Further analysis using cellulose acetate membrane electrophoresis showed good separation between species of sGAGs namely chondroitin/dermatan sulfate and heparan sulfate, with comparatively lesser interference from hyaluronic acid, a non-sulfated GAG.
29209879	8	12	theme	heparan	1246:1252	arg1	sulfate					1254:1260	heparan sulfate	1246:1260	heparan sulfate	1246:1260	Further analysis using cellulose acetate membrane electrophoresis showed good separation between species of sGAGs namely chondroitin/dermatan sulfate and heparan sulfate, with comparatively lesser interference from hyaluronic acid, a non-sulfated GAG.
29209879	6	13	theme	membrane	862:869	arg1	electrophoresis					871:885	cellulose acetate membrane electrophoresis	844:885	cellulose acetate membrane electrophoresis	844:885	Recovered sGAGs were analysed by cellulose acetate membrane electrophoresis and taken up for disaccharide composition analysis by HPLC after fluorescent labelling.
29209879	3	14	theme	composition	465:475	arg1	estimation					422:431	estimation	422:431	estimation	422:431	In such a scenario, the sample which can be made available for estimation and elucidation of disaccharide composition and species composition as well remains a challenge.
29209879	3	14	theme	composition	465:475	arg1	elucidation					437:447	elucidation	437:447	elucidation	437:447	In such a scenario, the sample which can be made available for estimation and elucidation of disaccharide composition and species composition as well remains a challenge.
29209879	8	15	theme	sGAGs	1200:1204	arg1	separation					1170:1179	good separation	1165:1179	good separation between species of sGAGs namely chondroitin/dermatan sulfate and heparan sulfate	1165:1260	Further analysis using cellulose acetate membrane electrophoresis showed good separation between species of sGAGs namely chondroitin/dermatan sulfate and heparan sulfate, with comparatively lesser interference from hyaluronic acid, a non-sulfated GAG.
29209879	6	16	theme	acetate	854:860	arg1	electrophoresis					871:885	cellulose acetate membrane electrophoresis	844:885	cellulose acetate membrane electrophoresis	844:885	Recovered sGAGs were analysed by cellulose acetate membrane electrophoresis and taken up for disaccharide composition analysis by HPLC after fluorescent labelling.
29209879	10	17	theme	purer	1596:1600	arg1	form					1602:1605	comparatively purer form	1582:1605	comparatively purer form	1582:1605	Thus, in the present paper, we show that sGAG can be recovered in comparatively purer form after routine estimation and can be used for further analysis thus saving up on the precious sample.
29209879	10	18	theme	routine	1613:1619	arg1	estimation					1621:1630	routine estimation	1613:1630	routine estimation	1613:1630	Thus, in the present paper, we show that sGAG can be recovered in comparatively purer form after routine estimation and can be used for further analysis thus saving up on the precious sample.
29209879	8	19	theme	lesser	1282:1287	arg1	interference					1289:1300	comparatively lesser interference	1268:1300	comparatively lesser interference from hyaluronic acid, a non-sulfated GAG	1268:1341	Further analysis using cellulose acetate membrane electrophoresis showed good separation between species of sGAGs namely chondroitin/dermatan sulfate and heparan sulfate, with comparatively lesser interference from hyaluronic acid, a non-sulfated GAG.
29209879	0	20	theme	One-pot	0:6	arg1	analysis					8:15	One-pot analysis	0:15	One-pot analysis of sulfated glycosaminoglycans	0:46	One-pot analysis of sulfated glycosaminoglycans.
29209879	9	21	theme	sGAGs	1366:1370	arg1	Analysis					1344:1351	Analysis	1344:1351	Analysis of recovered sGAGs, specifically heparan sulfate by HPLC	1344:1408	Analysis of recovered sGAGs, specifically heparan sulfate by HPLC showed characteristic disaccharide composition akin to that of GAG obtained by the conventional protocol.
29209879	2	22	theme	available	290:298	arg1	GAGs					285:288	GAGs	285:288	GAGs available for various structural, functional and biological studies	285:356	This is compounded by the fact that the analysis is technique-intensive and more often there will be a limitation on the quantity of GAGs available for various structural, functional and biological studies.
29209879	9	23	theme	conventional	1493:1504	arg1	protocol					1506:1513	the conventional protocol	1489:1513	the conventional protocol	1489:1513	Analysis of recovered sGAGs, specifically heparan sulfate by HPLC showed characteristic disaccharide composition akin to that of GAG obtained by the conventional protocol.
29209879	0	24	theme	sulfated	20:27	arg1	glycosaminoglycans					29:46	sulfated glycosaminoglycans	20:46	sulfated glycosaminoglycans	20:46	One-pot analysis of sulfated glycosaminoglycans.
29209879	8	25	theme	non-sulfated	1326:1337	arg1	GAG					1339:1341	a non-sulfated GAG	1324:1341	a non-sulfated GAG	1324:1341	Further analysis using cellulose acetate membrane electrophoresis showed good separation between species of sGAGs namely chondroitin/dermatan sulfate and heparan sulfate, with comparatively lesser interference from hyaluronic acid, a non-sulfated GAG.
29209879	8	25	theme	non-sulfated	1326:1337	arg1	acid					1318:1321	hyaluronic acid	1307:1321	hyaluronic acid	1307:1321	Further analysis using cellulose acetate membrane electrophoresis showed good separation between species of sGAGs namely chondroitin/dermatan sulfate and heparan sulfate, with comparatively lesser interference from hyaluronic acid, a non-sulfated GAG.
29209879	2	26	theme	GAGs	285:288	arg1	quantity					273:280	the quantity	269:280	the quantity of GAGs available for various structural, functional and biological studies	269:356	This is compounded by the fact that the analysis is technique-intensive and more often there will be a limitation on the quantity of GAGs available for various structural, functional and biological studies.
29209879	10	27	theme	precious	1691:1698	arg1	sample					1700:1705	the precious sample	1687:1705	the precious sample	1687:1705	Thus, in the present paper, we show that sGAG can be recovered in comparatively purer form after routine estimation and can be used for further analysis thus saving up on the precious sample.
29209879	6	28	theme	fluorescent	952:962	arg1	labelling					964:972	fluorescent labelling	952:972	fluorescent labelling	952:972	Recovered sGAGs were analysed by cellulose acetate membrane electrophoresis and taken up for disaccharide composition analysis by HPLC after fluorescent labelling.
29209879	7	29	theme	purity	1067:1072	arg1	levels					1057:1062	varying levels	1049:1062	varying levels of purity	1049:1072	Good recovery of sGAGs after metachromasia was observed in all samples of varying levels of purity by this protocol.
29209879	6	30	theme	cellulose	844:852	arg1	electrophoresis					871:885	cellulose acetate membrane electrophoresis	844:885	cellulose acetate membrane electrophoresis	844:885	Recovered sGAGs were analysed by cellulose acetate membrane electrophoresis and taken up for disaccharide composition analysis by HPLC after fluorescent labelling.
29209879	2	31	dep	technique-intensive	204:222	arg1	limitation					255:264	a limitation	253:264	a limitation on the quantity of GAGs available for various structural, functional and biological studies	253:356	This is compounded by the fact that the analysis is technique-intensive and more often there will be a limitation on the quantity of GAGs available for various structural, functional and biological studies.
29209879	2	31	dep	technique-intensive	204:222	arg1	analysis					192:199	the analysis	188:199	the analysis	188:199	This is compounded by the fact that the analysis is technique-intensive and more often there will be a limitation on the quantity of GAGs available for various structural, functional and biological studies.
29209879	2	31	dep	technique-intensive	204:222	arg1	technique-intensive					204:222	technique-intensive	204:222	technique-intensive	204:222	This is compounded by the fact that the analysis is technique-intensive and more often there will be a limitation on the quantity of GAGs available for various structural, functional and biological studies.
29209879	5	32	theme	DMMB	746:749	arg1	assay					763:767	DMMB dye-binding assay	746:767	DMMB dye-binding assay	746:767	sGAG-DMMB complex formed after estimation of sGAG by DMMB dye-binding assay was decomplexed and sGAGs were recovered.
29209879	4	33	theme	present	537:543	arg1	study					545:549	the present study	533:549	the present study	533:549	In the present study, we have determined the feasibility where isolated sulfated GAGs (sGAG) that is estimated by metachromasia is recovered for further analysis.
29209879	7	34	theme	varying	1049:1055	arg1	levels					1057:1062	varying levels	1049:1062	varying levels of purity	1049:1072	Good recovery of sGAGs after metachromasia was observed in all samples of varying levels of purity by this protocol.
29209879	1	35	theme	glycosaminoglycans	104:121	arg1	estimation					68:77	estimation	68:77	estimation	68:77	Routine isolation, estimation, and characterization of glycosaminoglycans (GAGs) is quite challenging.
29209879	1	35	theme	glycosaminoglycans	104:121	arg1	characterization					84:99	characterization	84:99	characterization	84:99	Routine isolation, estimation, and characterization of glycosaminoglycans (GAGs) is quite challenging.
29209879	1	35	theme	glycosaminoglycans	104:121	arg1	isolation					57:65	Routine isolation	49:65	Routine isolation	49:65	Routine isolation, estimation, and characterization of glycosaminoglycans (GAGs) is quite challenging.
29209879	0	36	theme	glycosaminoglycans	29:46	arg1	analysis					8:15	One-pot analysis	0:15	One-pot analysis of sulfated glycosaminoglycans	0:46	One-pot analysis of sulfated glycosaminoglycans.
29209879	5	37	theme	dye-binding	751:761	arg1	assay					763:767	DMMB dye-binding assay	746:767	DMMB dye-binding assay	746:767	sGAG-DMMB complex formed after estimation of sGAG by DMMB dye-binding assay was decomplexed and sGAGs were recovered.
29209879	8	38	theme	chondroitin/dermatan	1213:1232	arg1	sGAGs					1200:1204	sGAGs	1200:1204	sGAGs namely chondroitin/dermatan sulfate and heparan sulfate	1200:1260	Further analysis using cellulose acetate membrane electrophoresis showed good separation between species of sGAGs namely chondroitin/dermatan sulfate and heparan sulfate, with comparatively lesser interference from hyaluronic acid, a non-sulfated GAG.
29209879	8	38	theme	chondroitin/dermatan	1213:1232	arg1	sulfate					1234:1240	chondroitin/dermatan sulfate	1213:1240	chondroitin/dermatan sulfate	1213:1240	Further analysis using cellulose acetate membrane electrophoresis showed good separation between species of sGAGs namely chondroitin/dermatan sulfate and heparan sulfate, with comparatively lesser interference from hyaluronic acid, a non-sulfated GAG.
29209879	8	39	theme	hyaluronic	1307:1316	arg1	GAG					1339:1341	a non-sulfated GAG	1324:1341	a non-sulfated GAG	1324:1341	Further analysis using cellulose acetate membrane electrophoresis showed good separation between species of sGAGs namely chondroitin/dermatan sulfate and heparan sulfate, with comparatively lesser interference from hyaluronic acid, a non-sulfated GAG.
29209879	8	39	theme	hyaluronic	1307:1316	arg1	acid					1318:1321	hyaluronic acid	1307:1321	hyaluronic acid	1307:1321	Further analysis using cellulose acetate membrane electrophoresis showed good separation between species of sGAGs namely chondroitin/dermatan sulfate and heparan sulfate, with comparatively lesser interference from hyaluronic acid, a non-sulfated GAG.
29209879	7	40	located	observed	1022:1029	arg1	samples					1038:1044	all samples	1034:1044	all samples of varying levels of purity	1034:1072	Good recovery of sGAGs after metachromasia was observed in all samples of varying levels of purity by this protocol.
29209879	7	40	located	observed	1022:1029	arg2	recovery					980:987	Good recovery	975:987	Good recovery of sGAGs after metachromasia	975:1016	Good recovery of sGAGs after metachromasia was observed in all samples of varying levels of purity by this protocol.
29209879	10	41	used	used	1643:1646	arg2	sGAG					1557:1560	sGAG	1557:1560	sGAG	1557:1560	Thus, in the present paper, we show that sGAG can be recovered in comparatively purer form after routine estimation and can be used for further analysis thus saving up on the precious sample.
29209879	8	42	dep	sGAGs	1200:1204	arg1	sGAGs					1200:1204	sGAGs	1200:1204	sGAGs namely chondroitin/dermatan sulfate and heparan sulfate	1200:1260	Further analysis using cellulose acetate membrane electrophoresis showed good separation between species of sGAGs namely chondroitin/dermatan sulfate and heparan sulfate, with comparatively lesser interference from hyaluronic acid, a non-sulfated GAG.
29209879	8	42	dep	sGAGs	1200:1204	arg1	sulfate					1234:1240	chondroitin/dermatan sulfate	1213:1240	chondroitin/dermatan sulfate	1213:1240	Further analysis using cellulose acetate membrane electrophoresis showed good separation between species of sGAGs namely chondroitin/dermatan sulfate and heparan sulfate, with comparatively lesser interference from hyaluronic acid, a non-sulfated GAG.
29209879	8	42	dep	sGAGs	1200:1204	arg1	sulfate					1254:1260	heparan sulfate	1246:1260	heparan sulfate	1246:1260	Further analysis using cellulose acetate membrane electrophoresis showed good separation between species of sGAGs namely chondroitin/dermatan sulfate and heparan sulfate, with comparatively lesser interference from hyaluronic acid, a non-sulfated GAG.
29209879	4	43	theme	further	675:681	arg1	analysis					683:690	further analysis	675:690	further analysis	675:690	In the present study, we have determined the feasibility where isolated sulfated GAGs (sGAG) that is estimated by metachromasia is recovered for further analysis.
29209879	7	44	theme	levels	1057:1062	arg1	samples					1038:1044	all samples	1034:1044	all samples of varying levels of purity	1034:1072	Good recovery of sGAGs after metachromasia was observed in all samples of varying levels of purity by this protocol.
29209879	8	45	theme	Further	1092:1098	arg1	analysis					1100:1107	Further analysis	1092:1107	Further analysis using cellulose acetate membrane electrophoresis	1092:1156	Further analysis using cellulose acetate membrane electrophoresis showed good separation between species of sGAGs namely chondroitin/dermatan sulfate and heparan sulfate, with comparatively lesser interference from hyaluronic acid, a non-sulfated GAG.
29209879	10	46	from	saving	1674:1679	arg1	sample					1700:1705	the precious sample	1687:1705	the precious sample	1687:1705	Thus, in the present paper, we show that sGAG can be recovered in comparatively purer form after routine estimation and can be used for further analysis thus saving up on the precious sample.
29209879	6	47	theme	composition	917:927	arg1	analysis					929:936	disaccharide composition analysis	904:936	disaccharide composition analysis by HPLC	904:944	Recovered sGAGs were analysed by cellulose acetate membrane electrophoresis and taken up for disaccharide composition analysis by HPLC after fluorescent labelling.
29209879	8	48	theme	cellulose	1115:1123	arg1	electrophoresis					1142:1156	cellulose acetate membrane electrophoresis	1115:1156	cellulose acetate membrane electrophoresis	1115:1156	Further analysis using cellulose acetate membrane electrophoresis showed good separation between species of sGAGs namely chondroitin/dermatan sulfate and heparan sulfate, with comparatively lesser interference from hyaluronic acid, a non-sulfated GAG.
29209879	6	49	theme	Recovered	811:819	arg1	sGAGs					821:825	Recovered sGAGs	811:825	Recovered sGAGs	811:825	Recovered sGAGs were analysed by cellulose acetate membrane electrophoresis and taken up for disaccharide composition analysis by HPLC after fluorescent labelling.
29209879	9	50	theme	heparan	1386:1392	arg1	sGAGs					1366:1370	recovered sGAGs	1356:1370	recovered sGAGs	1356:1370	Analysis of recovered sGAGs, specifically heparan sulfate by HPLC showed characteristic disaccharide composition akin to that of GAG obtained by the conventional protocol.
29209879	9	50	theme	heparan	1386:1392	arg1	sulfate					1394:1400	heparan sulfate	1386:1400	specifically heparan sulfate	1373:1400	Analysis of recovered sGAGs, specifically heparan sulfate by HPLC showed characteristic disaccharide composition akin to that of GAG obtained by the conventional protocol.
29209879	10	51	dep	analysis	1660:1667	arg1	saving					1674:1679	saving	1674:1679	saving	1674:1679	Thus, in the present paper, we show that sGAG can be recovered in comparatively purer form after routine estimation and can be used for further analysis thus saving up on the precious sample.
29209879	2	52	theme	structural	312:321	arg1	studies					350:356	various structural, functional and biological studies	304:356	various structural, functional and biological studies	304:356	This is compounded by the fact that the analysis is technique-intensive and more often there will be a limitation on the quantity of GAGs available for various structural, functional and biological studies.
29209879	2	53	from	limitation	255:264	arg1	quantity					273:280	the quantity	269:280	the quantity of GAGs available for various structural, functional and biological studies	269:356	This is compounded by the fact that the analysis is technique-intensive and more often there will be a limitation on the quantity of GAGs available for various structural, functional and biological studies.
29209879	9	54	theme	characteristic	1417:1430	arg1	composition					1445:1455	characteristic disaccharide composition	1417:1455	characteristic disaccharide composition akin to that of GAG obtained by the conventional protocol	1417:1513	Analysis of recovered sGAGs, specifically heparan sulfate by HPLC showed characteristic disaccharide composition akin to that of GAG obtained by the conventional protocol.
29209879	8	55	from	acid	1318:1321	arg1	interference					1289:1300	comparatively lesser interference	1268:1300	comparatively lesser interference from hyaluronic acid, a non-sulfated GAG	1268:1341	Further analysis using cellulose acetate membrane electrophoresis showed good separation between species of sGAGs namely chondroitin/dermatan sulfate and heparan sulfate, with comparatively lesser interference from hyaluronic acid, a non-sulfated GAG.
29209879	2	56	theme	various	304:310	arg1	studies					350:356	various structural, functional and biological studies	304:356	various structural, functional and biological studies	304:356	This is compounded by the fact that the analysis is technique-intensive and more often there will be a limitation on the quantity of GAGs available for various structural, functional and biological studies.
29209879	9	57	theme	disaccharide	1432:1443	arg1	composition					1445:1455	characteristic disaccharide composition	1417:1455	characteristic disaccharide composition akin to that of GAG obtained by the conventional protocol	1417:1513	Analysis of recovered sGAGs, specifically heparan sulfate by HPLC showed characteristic disaccharide composition akin to that of GAG obtained by the conventional protocol.
29209879	8	58	theme	membrane	1133:1140	arg1	electrophoresis					1142:1156	cellulose acetate membrane electrophoresis	1115:1156	cellulose acetate membrane electrophoresis	1115:1156	Further analysis using cellulose acetate membrane electrophoresis showed good separation between species of sGAGs namely chondroitin/dermatan sulfate and heparan sulfate, with comparatively lesser interference from hyaluronic acid, a non-sulfated GAG.
29209879	1	59	theme	Routine	49:55	arg1	isolation					57:65	Routine isolation	49:65	Routine isolation	49:65	Routine isolation, estimation, and characterization of glycosaminoglycans (GAGs) is quite challenging.
29209879	7	60	theme	sGAGs	992:996	arg1	recovery					980:987	Good recovery	975:987	Good recovery of sGAGs after metachromasia	975:1016	Good recovery of sGAGs after metachromasia was observed in all samples of varying levels of purity by this protocol.
29209879	4	61	theme	isolated	593:600	arg1	sGAG					617:620	sGAG	617:620	sGAG	617:620	In the present study, we have determined the feasibility where isolated sulfated GAGs (sGAG) that is estimated by metachromasia is recovered for further analysis.
29209879	4	61	theme	isolated	593:600	arg1	GAGs					611:614	isolated sulfated GAGs	593:614	isolated sulfated GAGs (sGAG) that is estimated by metachromasia	593:656	In the present study, we have determined the feasibility where isolated sulfated GAGs (sGAG) that is estimated by metachromasia is recovered for further analysis.
29209879	8	62	theme	acetate	1125:1131	arg1	electrophoresis					1142:1156	cellulose acetate membrane electrophoresis	1115:1156	cellulose acetate membrane electrophoresis	1115:1156	Further analysis using cellulose acetate membrane electrophoresis showed good separation between species of sGAGs namely chondroitin/dermatan sulfate and heparan sulfate, with comparatively lesser interference from hyaluronic acid, a non-sulfated GAG.
29209879	6	63	theme	disaccharide	904:915	arg1	analysis					929:936	disaccharide composition analysis	904:936	disaccharide composition analysis by HPLC	904:944	Recovered sGAGs were analysed by cellulose acetate membrane electrophoresis and taken up for disaccharide composition analysis by HPLC after fluorescent labelling.
29209879	7	64	theme	Good	975:978	arg1	recovery					980:987	Good recovery	975:987	Good recovery of sGAGs after metachromasia	975:1016	Good recovery of sGAGs after metachromasia was observed in all samples of varying levels of purity by this protocol.
29209879	9	65	theme	akin	1457:1460	arg1	composition					1445:1455	characteristic disaccharide composition	1417:1455	characteristic disaccharide composition akin to that of GAG obtained by the conventional protocol	1417:1513	Analysis of recovered sGAGs, specifically heparan sulfate by HPLC showed characteristic disaccharide composition akin to that of GAG obtained by the conventional protocol.
28219735	6	0	theme	uncharacterized	641:655	arg1	EPS					657:659	This uncharacterized EPS	636:659	This uncharacterized EPS	636:659	This uncharacterized EPS may have use in commercial prebiotics, food additives, and medical purposes.
28219735	5	1	theme	spectrometry	603:614	arg1	pattern					616:622	the typical mass spectrometry pattern	586:622	the typical mass spectrometry pattern of dextran	586:633	The MALDI-TOF results displayed the typical mass spectrometry pattern of dextran.
28219735	1	2	theme	intensive	175:183	arg1	producer					185:192	an intensive producer	172:192	an intensive producer of exopolysaccharide (EPS)	172:219	Leuconostoc garlicum KCCM 43211 isolated from traditional Korean fermented food is an intensive producer of exopolysaccharide (EPS).
28219735	1	2	theme	intensive	175:183	arg1	43211					115:119	Leuconostoc garlicum KCCM 43211	89:119	Leuconostoc garlicum KCCM 43211 isolated from traditional Korean fermented food	89:167	Leuconostoc garlicum KCCM 43211 isolated from traditional Korean fermented food is an intensive producer of exopolysaccharide (EPS).
28219735	7	3	theme	complete	742:749	arg1	sequence					758:765	The complete genome sequence	738:765	The complete genome sequence of L. garlicum KCCM 43211	738:791	The complete genome sequence of L. garlicum KCCM 43211 will provide valuable information for strain engineering based on the genetic information.
28219735	2	4	theme	43211	292:296	arg1	sequence					263:270	the first complete genome sequence	237:270	the first complete genome sequence of L. garlicum KCCM 43211	237:296	Here we report the first complete genome sequence of L. garlicum KCCM 43211.
28219735	4	5	theme	43211	479:483	arg1	strains					485:491	the L. garlicum KCCM 43211 strains	458:491	the L. garlicum KCCM 43211 strains	458:491	An uncharacterized EPS from the L. garlicum KCCM 43211 strains was also produced during fermentation in the sucrose medium.
28219735	2	6	theme	KCCM	287:290	arg1	43211					292:296	L. garlicum KCCM 43211	275:296	L. garlicum KCCM 43211	275:296	Here we report the first complete genome sequence of L. garlicum KCCM 43211.
28219735	6	7	theme	medical	720:726	arg1	purposes					728:735	medical purposes	720:735	medical purposes	720:735	This uncharacterized EPS may have use in commercial prebiotics, food additives, and medical purposes.
28219735	4	8	theme	KCCM	474:477	arg1	strains					485:491	the L. garlicum KCCM 43211 strains	458:491	the L. garlicum KCCM 43211 strains	458:491	An uncharacterized EPS from the L. garlicum KCCM 43211 strains was also produced during fermentation in the sucrose medium.
28219735	5	9	theme	dextran	627:633	arg1	pattern					616:622	the typical mass spectrometry pattern	586:622	the typical mass spectrometry pattern of dextran	586:633	The MALDI-TOF results displayed the typical mass spectrometry pattern of dextran.
28219735	2	10	theme	garlicum	278:285	arg1	43211					292:296	L. garlicum KCCM 43211	275:296	L. garlicum KCCM 43211	275:296	Here we report the first complete genome sequence of L. garlicum KCCM 43211.
28219735	6	11	from	use	670:672	arg1	purposes					728:735	medical purposes	720:735	medical purposes	720:735	This uncharacterized EPS may have use in commercial prebiotics, food additives, and medical purposes.
28219735	6	11	from	use	670:672	arg1	prebiotics					688:697	commercial prebiotics	677:697	commercial prebiotics	677:697	This uncharacterized EPS may have use in commercial prebiotics, food additives, and medical purposes.
28219735	6	11	from	use	670:672	arg1	additives					705:713	food additives	700:713	food additives	700:713	This uncharacterized EPS may have use in commercial prebiotics, food additives, and medical purposes.
28219735	3	12	theme	glucose	412:418	arg1	monomers					420:427	glucose monomers	412:427	glucose monomers	412:427	The genome sequence displayed that this strain contains genes involved in production of EPS possibly composed of glucose monomers.
28219735	1	13	theme	Leuconostoc	89:99	arg1	producer					185:192	an intensive producer	172:192	an intensive producer of exopolysaccharide (EPS)	172:219	Leuconostoc garlicum KCCM 43211 isolated from traditional Korean fermented food is an intensive producer of exopolysaccharide (EPS).
28219735	1	13	theme	Leuconostoc	89:99	arg1	43211					115:119	Leuconostoc garlicum KCCM 43211	89:119	Leuconostoc garlicum KCCM 43211 isolated from traditional Korean fermented food	89:167	Leuconostoc garlicum KCCM 43211 isolated from traditional Korean fermented food is an intensive producer of exopolysaccharide (EPS).
28219735	7	14	theme	garlicum	773:780	arg1	43211					787:791	L. garlicum KCCM 43211	770:791	L. garlicum KCCM 43211	770:791	The complete genome sequence of L. garlicum KCCM 43211 will provide valuable information for strain engineering based on the genetic information.
28219735	0	15	theme	genome	9:14	arg1	sequence					16:23	Complete genome sequence	0:23	Complete genome sequence of Leuconostoc garlicum KCCM 43211	0:58	Complete genome sequence of Leuconostoc garlicum KCCM 43211 producing exopolysaccharide.
28219735	1	16	theme	garlicum	101:108	arg1	producer					185:192	an intensive producer	172:192	an intensive producer of exopolysaccharide (EPS)	172:219	Leuconostoc garlicum KCCM 43211 isolated from traditional Korean fermented food is an intensive producer of exopolysaccharide (EPS).
28219735	1	16	theme	garlicum	101:108	arg1	43211					115:119	Leuconostoc garlicum KCCM 43211	89:119	Leuconostoc garlicum KCCM 43211 isolated from traditional Korean fermented food	89:167	Leuconostoc garlicum KCCM 43211 isolated from traditional Korean fermented food is an intensive producer of exopolysaccharide (EPS).
28219735	7	17	theme	genetic	863:869	arg1	information					871:881	the genetic information	859:881	the genetic information	859:881	The complete genome sequence of L. garlicum KCCM 43211 will provide valuable information for strain engineering based on the genetic information.
28219735	1	18	theme	exopolysaccharide	197:213	arg1	producer					185:192	an intensive producer	172:192	an intensive producer of exopolysaccharide (EPS)	172:219	Leuconostoc garlicum KCCM 43211 isolated from traditional Korean fermented food is an intensive producer of exopolysaccharide (EPS).
28219735	1	18	theme	exopolysaccharide	197:213	arg1	43211					115:119	Leuconostoc garlicum KCCM 43211	89:119	Leuconostoc garlicum KCCM 43211 isolated from traditional Korean fermented food	89:167	Leuconostoc garlicum KCCM 43211 isolated from traditional Korean fermented food is an intensive producer of exopolysaccharide (EPS).
28219735	0	19	theme	Complete	0:7	arg1	sequence					16:23	Complete genome sequence	0:23	Complete genome sequence of Leuconostoc garlicum KCCM 43211	0:58	Complete genome sequence of Leuconostoc garlicum KCCM 43211 producing exopolysaccharide.
28219735	5	20	theme	typical	590:596	arg1	pattern					616:622	the typical mass spectrometry pattern	586:622	the typical mass spectrometry pattern of dextran	586:633	The MALDI-TOF results displayed the typical mass spectrometry pattern of dextran.
28219735	1	21	theme	KCCM	110:113	arg1	producer					185:192	an intensive producer	172:192	an intensive producer of exopolysaccharide (EPS)	172:219	Leuconostoc garlicum KCCM 43211 isolated from traditional Korean fermented food is an intensive producer of exopolysaccharide (EPS).
28219735	1	21	theme	KCCM	110:113	arg1	43211					115:119	Leuconostoc garlicum KCCM 43211	89:119	Leuconostoc garlicum KCCM 43211 isolated from traditional Korean fermented food	89:167	Leuconostoc garlicum KCCM 43211 isolated from traditional Korean fermented food is an intensive producer of exopolysaccharide (EPS).
28219735	6	22	theme	food	700:703	arg1	additives					705:713	food additives	700:713	food additives	700:713	This uncharacterized EPS may have use in commercial prebiotics, food additives, and medical purposes.
28219735	5	23	theme	mass	598:601	arg1	pattern					616:622	the typical mass spectrometry pattern	586:622	the typical mass spectrometry pattern of dextran	586:633	The MALDI-TOF results displayed the typical mass spectrometry pattern of dextran.
28219735	6	24	contain	have	665:668	arg1	EPS					657:659	This uncharacterized EPS	636:659	This uncharacterized EPS	636:659	This uncharacterized EPS may have use in commercial prebiotics, food additives, and medical purposes.
28219735	6	24	contain	have	665:668	arg2	use					670:672	use	670:672	use in commercial prebiotics, food additives, and medical purposes	670:735	This uncharacterized EPS may have use in commercial prebiotics, food additives, and medical purposes.
28219735	7	25	theme	strain	831:836	arg1	engineering					838:848	strain engineering	831:848	strain engineering	831:848	The complete genome sequence of L. garlicum KCCM 43211 will provide valuable information for strain engineering based on the genetic information.
28219735	0	26	theme	garlicum	40:47	arg1	KCCM					49:52	Leuconostoc garlicum KCCM 43211	28:58	Leuconostoc garlicum KCCM 43211	28:58	Complete genome sequence of Leuconostoc garlicum KCCM 43211 producing exopolysaccharide.
28219735	7	27	theme	valuable	806:813	arg1	information					815:825	valuable information	806:825	valuable information for strain engineering based on the genetic information	806:881	The complete genome sequence of L. garlicum KCCM 43211 will provide valuable information for strain engineering based on the genetic information.
28219735	2	28	theme	first	241:245	arg1	sequence					263:270	the first complete genome sequence	237:270	the first complete genome sequence of L. garlicum KCCM 43211	237:296	Here we report the first complete genome sequence of L. garlicum KCCM 43211.
28219735	6	29	theme	commercial	677:686	arg1	prebiotics					688:697	commercial prebiotics	677:697	commercial prebiotics	677:697	This uncharacterized EPS may have use in commercial prebiotics, food additives, and medical purposes.
28219735	0	30	theme	Leuconostoc	28:38	arg1	KCCM					49:52	Leuconostoc garlicum KCCM 43211	28:58	Leuconostoc garlicum KCCM 43211	28:58	Complete genome sequence of Leuconostoc garlicum KCCM 43211 producing exopolysaccharide.
28219735	7	31	theme	43211	787:791	arg1	sequence					758:765	The complete genome sequence	738:765	The complete genome sequence of L. garlicum KCCM 43211	738:791	The complete genome sequence of L. garlicum KCCM 43211 will provide valuable information for strain engineering based on the genetic information.
28219735	4	32	theme	garlicum	465:472	arg1	strains					485:491	the L. garlicum KCCM 43211 strains	458:491	the L. garlicum KCCM 43211 strains	458:491	An uncharacterized EPS from the L. garlicum KCCM 43211 strains was also produced during fermentation in the sucrose medium.
28219735	7	33	theme	KCCM	782:785	arg1	43211					787:791	L. garlicum KCCM 43211	770:791	L. garlicum KCCM 43211	770:791	The complete genome sequence of L. garlicum KCCM 43211 will provide valuable information for strain engineering based on the genetic information.
28219735	3	34	theme	genome	303:308	arg1	sequence					310:317	The genome sequence	299:317	The genome sequence	299:317	The genome sequence displayed that this strain contains genes involved in production of EPS possibly composed of glucose monomers.
28219735	4	35	theme	L.	462:463	arg1	strains					485:491	the L. garlicum KCCM 43211 strains	458:491	the L. garlicum KCCM 43211 strains	458:491	An uncharacterized EPS from the L. garlicum KCCM 43211 strains was also produced during fermentation in the sucrose medium.
28219735	3	36	contain	contains	346:353	arg2	genes					355:359	genes	355:359	genes involved in production of EPS possibly composed of glucose monomers	355:427	The genome sequence displayed that this strain contains genes involved in production of EPS possibly composed of glucose monomers.
28219735	3	36	contain	contains	346:353	arg1	strain					339:344	this strain	334:344	this strain	334:344	The genome sequence displayed that this strain contains genes involved in production of EPS possibly composed of glucose monomers.
28219735	0	37	theme	KCCM	49:52	arg1	sequence					16:23	Complete genome sequence	0:23	Complete genome sequence of Leuconostoc garlicum KCCM 43211	0:58	Complete genome sequence of Leuconostoc garlicum KCCM 43211 producing exopolysaccharide.
28219735	1	38	attach	isolated	121:128	arg2	producer					185:192	an intensive producer	172:192	an intensive producer of exopolysaccharide (EPS)	172:219	Leuconostoc garlicum KCCM 43211 isolated from traditional Korean fermented food is an intensive producer of exopolysaccharide (EPS).
28219735	1	38	attach	isolated	121:128	arg2	43211					115:119	Leuconostoc garlicum KCCM 43211	89:119	Leuconostoc garlicum KCCM 43211 isolated from traditional Korean fermented food	89:167	Leuconostoc garlicum KCCM 43211 isolated from traditional Korean fermented food is an intensive producer of exopolysaccharide (EPS).
28219735	1	38	attach	isolated	121:128	arg1	food					164:167	traditional Korean fermented food	135:167	traditional Korean fermented food	135:167	Leuconostoc garlicum KCCM 43211 isolated from traditional Korean fermented food is an intensive producer of exopolysaccharide (EPS).
28219735	4	39	from	strains	485:491	arg1	EPS					449:451	An uncharacterized EPS	430:451	An uncharacterized EPS from the L. garlicum KCCM 43211 strains	430:491	An uncharacterized EPS from the L. garlicum KCCM 43211 strains was also produced during fermentation in the sucrose medium.
28219735	5	40	theme	MALDI-TOF	558:566	arg1	results					568:574	The MALDI-TOF results	554:574	The MALDI-TOF results	554:574	The MALDI-TOF results displayed the typical mass spectrometry pattern of dextran.
28219735	1	41	theme	traditional	135:145	arg1	food					164:167	traditional Korean fermented food	135:167	traditional Korean fermented food	135:167	Leuconostoc garlicum KCCM 43211 isolated from traditional Korean fermented food is an intensive producer of exopolysaccharide (EPS).
28219735	4	42	theme	sucrose	538:544	arg1	medium					546:551	the sucrose medium	534:551	the sucrose medium	534:551	An uncharacterized EPS from the L. garlicum KCCM 43211 strains was also produced during fermentation in the sucrose medium.
28219735	1	43	theme	Korean	147:152	arg1	food					164:167	traditional Korean fermented food	135:167	traditional Korean fermented food	135:167	Leuconostoc garlicum KCCM 43211 isolated from traditional Korean fermented food is an intensive producer of exopolysaccharide (EPS).
28219735	2	44	theme	L.	275:276	arg1	43211					292:296	L. garlicum KCCM 43211	275:296	L. garlicum KCCM 43211	275:296	Here we report the first complete genome sequence of L. garlicum KCCM 43211.
28219735	1	45	theme	fermented	154:162	arg1	food					164:167	traditional Korean fermented food	135:167	traditional Korean fermented food	135:167	Leuconostoc garlicum KCCM 43211 isolated from traditional Korean fermented food is an intensive producer of exopolysaccharide (EPS).
28219735	7	46	theme	L.	770:771	arg1	43211					787:791	L. garlicum KCCM 43211	770:791	L. garlicum KCCM 43211	770:791	The complete genome sequence of L. garlicum KCCM 43211 will provide valuable information for strain engineering based on the genetic information.
28219735	4	47	theme	uncharacterized	433:447	arg1	EPS					449:451	An uncharacterized EPS	430:451	An uncharacterized EPS from the L. garlicum KCCM 43211 strains	430:491	An uncharacterized EPS from the L. garlicum KCCM 43211 strains was also produced during fermentation in the sucrose medium.
28219735	2	48	theme	genome	256:261	arg1	sequence					263:270	the first complete genome sequence	237:270	the first complete genome sequence of L. garlicum KCCM 43211	237:296	Here we report the first complete genome sequence of L. garlicum KCCM 43211.
28219735	7	49	theme	genome	751:756	arg1	sequence					758:765	The complete genome sequence	738:765	The complete genome sequence of L. garlicum KCCM 43211	738:791	The complete genome sequence of L. garlicum KCCM 43211 will provide valuable information for strain engineering based on the genetic information.
28219735	2	50	theme	complete	247:254	arg1	sequence					263:270	the first complete genome sequence	237:270	the first complete genome sequence of L. garlicum KCCM 43211	237:296	Here we report the first complete genome sequence of L. garlicum KCCM 43211.
28219735	3	51	theme	EPS	387:389	arg1	production					373:382	production	373:382	production of EPS possibly composed of glucose monomers	373:427	The genome sequence displayed that this strain contains genes involved in production of EPS possibly composed of glucose monomers.
25629918	4	0	theme	crystal	866:872	arg1	structure					874:882	a B-type crystal structure	857:882	a B-type crystal structure observed by wide-angle X-ray scattering analysis	857:931	The nanomorphology of the composites showed the MPS having semicrystalline structure of a characteristic lamellar arrangement with an approximately 100 Å period observed by small-angle X-ray scattering and a B-type crystal structure observed by wide-angle X-ray scattering analysis.
25629918	1	1	theme	novel	236:240	arg1	MPS					278:280	MPS	278:280	MPS	278:280	In the present study, we were able to produce composites of wheat gluten (WG) protein and a novel genetically modified potato starch (MPS) with attractive mechanical and gas barrier properties using extrusion.
25629918	1	1	theme	novel	236:240	arg1	starch					270:275	a novel genetically modified potato starch	234:275	a novel genetically modified potato starch (MPS) with attractive mechanical and gas barrier properties using extrusion	234:351	In the present study, we were able to produce composites of wheat gluten (WG) protein and a novel genetically modified potato starch (MPS) with attractive mechanical and gas barrier properties using extrusion.
25629918	4	2	theme	small-angle	824:834	arg1	scattering					842:851	small-angle X-ray scattering	824:851	small-angle X-ray scattering	824:851	The nanomorphology of the composites showed the MPS having semicrystalline structure of a characteristic lamellar arrangement with an approximately 100 Å period observed by small-angle X-ray scattering and a B-type crystal structure observed by wide-angle X-ray scattering analysis.
25629918	1	3	with	starch	270:275	arg1	properties					326:335	attractive mechanical and gas barrier properties	288:335	properties	326:335	In the present study, we were able to produce composites of wheat gluten (WG) protein and a novel genetically modified potato starch (MPS) with attractive mechanical and gas barrier properties using extrusion.
25629918	1	3	with	starch	270:275	arg1	mechanical					299:308	mechanical	299:308	mechanical	299:308	In the present study, we were able to produce composites of wheat gluten (WG) protein and a novel genetically modified potato starch (MPS) with attractive mechanical and gas barrier properties using extrusion.
25629918	6	4	theme	45	1145:1146	arg1	%					1147:1147	%	1147:1147	%	1147:1147	A larger amount of β-sheets was observed in the samples 70/30 and 30/70 WG-MPS processed at 130 °C with 45% glycerol.
25629918	3	5	theme	different	546:554	arg1	ratios					556:561	different ratios	546:561	different ratios plasticized with either glycerol or glycerol and water	546:616	WG and MPS of different ratios plasticized with either glycerol or glycerol and water were extruded at 110 and 130 °C.
25629918	4	6	theme	scattering	913:922	arg1	analysis					924:931	wide-angle X-ray scattering analysis	896:931	wide-angle X-ray scattering analysis	896:931	The nanomorphology of the composites showed the MPS having semicrystalline structure of a characteristic lamellar arrangement with an approximately 100 Å period observed by small-angle X-ray scattering and a B-type crystal structure observed by wide-angle X-ray scattering analysis.
25629918	0	7	theme	barrier	124:130	arg1	properties					132:141	mechanical and barrier properties	109:141	properties	132:141	Nanostructural morphology of plasticized wheat gluten and modified potato starch composites: relationship to mechanical and barrier properties.
25629918	4	8	dep	period	805:810	arg1	Å					803:803	Å	803:803	Å	803:803	The nanomorphology of the composites showed the MPS having semicrystalline structure of a characteristic lamellar arrangement with an approximately 100 Å period observed by small-angle X-ray scattering and a B-type crystal structure observed by wide-angle X-ray scattering analysis.
25629918	7	9	theme	protein	1277:1283	arg1	amounts					1263:1269	greater amounts	1255:1269	greater amounts of WG protein in the blend	1255:1296	Highly polymerized WG protein was found in the samples processed at 130 °C versus 110 °C. Also, greater amounts of WG protein in the blend resulted in greater extensibility (110 °C) and a decrease in both E-modulus and maximum stress at 110 and 130 °C, respectively.
25629918	7	9	theme	protein	1277:1283	arg1	protein					1277:1283	WG protein	1274:1283	WG protein in the blend	1274:1296	Highly polymerized WG protein was found in the samples processed at 130 °C versus 110 °C. Also, greater amounts of WG protein in the blend resulted in greater extensibility (110 °C) and a decrease in both E-modulus and maximum stress at 110 and 130 °C, respectively.
25629918	5	10	dep	arrangement	993:1003	arg1	reported					1008:1015	reported	1008:1015	reported previously in WG films	1008:1038	WG has a structure resembling the hexagonal macromolecular arrangement as reported previously in WG films.
25629918	1	11	theme	wheat	204:208	arg1	gluten					210:215	wheat gluten	204:215	wheat gluten (WG) protein	204:228	In the present study, we were able to produce composites of wheat gluten (WG) protein and a novel genetically modified potato starch (MPS) with attractive mechanical and gas barrier properties using extrusion.
25629918	7	12	from	°C	1408:1409	arg1	decrease					1347:1354	a decrease	1345:1354	a decrease in both E-modulus and maximum stress at 110 and 130 °C	1345:1409	Highly polymerized WG protein was found in the samples processed at 130 °C versus 110 °C. Also, greater amounts of WG protein in the blend resulted in greater extensibility (110 °C) and a decrease in both E-modulus and maximum stress at 110 and 130 °C, respectively.
25629918	7	12	from	°C	1408:1409	arg1	extensibility					1318:1330	greater extensibility	1310:1330	greater extensibility (110 °C)	1310:1339	Highly polymerized WG protein was found in the samples processed at 130 °C versus 110 °C. Also, greater amounts of WG protein in the blend resulted in greater extensibility (110 °C) and a decrease in both E-modulus and maximum stress at 110 and 130 °C, respectively.
25629918	7	12	from	°C	1408:1409	arg1	°C					1337:1338	110 °C	1333:1338	110 °C	1333:1338	Highly polymerized WG protein was found in the samples processed at 130 °C versus 110 °C. Also, greater amounts of WG protein in the blend resulted in greater extensibility (110 °C) and a decrease in both E-modulus and maximum stress at 110 and 130 °C, respectively.
25629918	4	13	theme	composites	677:686	arg1	nanomorphology					655:668	The nanomorphology	651:668	The nanomorphology of the composites	651:686	The nanomorphology of the composites showed the MPS having semicrystalline structure of a characteristic lamellar arrangement with an approximately 100 Å period observed by small-angle X-ray scattering and a B-type crystal structure observed by wide-angle X-ray scattering analysis.
25629918	4	14	theme	wide-angle	896:905	arg1	analysis					924:931	wide-angle X-ray scattering analysis	896:931	wide-angle X-ray scattering analysis	896:931	The nanomorphology of the composites showed the MPS having semicrystalline structure of a characteristic lamellar arrangement with an approximately 100 Å period observed by small-angle X-ray scattering and a B-type crystal structure observed by wide-angle X-ray scattering analysis.
25629918	1	15	from	able	174:177	arg1	study					159:163	the present study	147:163	the present study	147:163	In the present study, we were able to produce composites of wheat gluten (WG) protein and a novel genetically modified potato starch (MPS) with attractive mechanical and gas barrier properties using extrusion.
25629918	2	16	theme	content	487:493	arg1	distribution					415:426	an altered chain length distribution	391:426	an altered chain length distribution of the amylopectin fraction and slightly increased amylose content compared to wild type potato starch	391:529	Characterization of the MPS revealed an altered chain length distribution of the amylopectin fraction and slightly increased amylose content compared to wild type potato starch.
25629918	0	17	theme	potato	67:72	arg1	composites					81:90	plasticized wheat gluten and modified potato starch composites	29:90	plasticized wheat gluten and modified potato starch composites	29:90	Nanostructural morphology of plasticized wheat gluten and modified potato starch composites: relationship to mechanical and barrier properties.
25629918	1	18	theme	present	151:157	arg1	study					159:163	the present study	147:163	the present study	147:163	In the present study, we were able to produce composites of wheat gluten (WG) protein and a novel genetically modified potato starch (MPS) with attractive mechanical and gas barrier properties using extrusion.
25629918	2	19	theme	increased	469:477	arg1	content					487:493	slightly increased amylose content	460:493	slightly increased amylose content	460:493	Characterization of the MPS revealed an altered chain length distribution of the amylopectin fraction and slightly increased amylose content compared to wild type potato starch.
25629918	0	20	theme	composites	81:90	arg1	morphology					15:24	Nanostructural morphology	0:24	Nanostructural morphology of plasticized wheat gluten and modified potato starch composites: relationship to mechanical and barrier properties.	0:142	Nanostructural morphology of plasticized wheat gluten and modified potato starch composites: relationship to mechanical and barrier properties.
25629918	1	21	from	study	159:163	arg1	able					174:177	able	174:177	able	174:177	In the present study, we were able to produce composites of wheat gluten (WG) protein and a novel genetically modified potato starch (MPS) with attractive mechanical and gas barrier properties using extrusion.
25629918	2	22	theme	type	512:515	arg1	starch					524:529	wild type potato starch	507:529	wild type potato starch	507:529	Characterization of the MPS revealed an altered chain length distribution of the amylopectin fraction and slightly increased amylose content compared to wild type potato starch.
25629918	1	23	theme	barrier	318:324	arg1	properties					326:335	attractive mechanical and gas barrier properties	288:335	properties	326:335	In the present study, we were able to produce composites of wheat gluten (WG) protein and a novel genetically modified potato starch (MPS) with attractive mechanical and gas barrier properties using extrusion.
25629918	7	24	from	blend	1292:1296	arg1	amounts					1263:1269	greater amounts	1255:1269	greater amounts of WG protein in the blend	1255:1296	Highly polymerized WG protein was found in the samples processed at 130 °C versus 110 °C. Also, greater amounts of WG protein in the blend resulted in greater extensibility (110 °C) and a decrease in both E-modulus and maximum stress at 110 and 130 °C, respectively.
25629918	7	24	from	blend	1292:1296	arg1	protein					1277:1283	WG protein	1274:1283	WG protein in the blend	1274:1296	Highly polymerized WG protein was found in the samples processed at 130 °C versus 110 °C. Also, greater amounts of WG protein in the blend resulted in greater extensibility (110 °C) and a decrease in both E-modulus and maximum stress at 110 and 130 °C, respectively.
25629918	4	25	theme	lamellar	756:763	arg1	arrangement					765:775	a characteristic lamellar arrangement	739:775	a characteristic lamellar arrangement	739:775	The nanomorphology of the composites showed the MPS having semicrystalline structure of a characteristic lamellar arrangement with an approximately 100 Å period observed by small-angle X-ray scattering and a B-type crystal structure observed by wide-angle X-ray scattering analysis.
25629918	0	26	theme	mechanical	109:118	arg1	properties					132:141	mechanical and barrier properties	109:141	properties	132:141	Nanostructural morphology of plasticized wheat gluten and modified potato starch composites: relationship to mechanical and barrier properties.
25629918	5	27	theme	macromolecular	978:991	arg1	arrangement					993:1003	the hexagonal macromolecular arrangement	964:1003	the hexagonal macromolecular arrangement as reported previously in WG films	964:1038	WG has a structure resembling the hexagonal macromolecular arrangement as reported previously in WG films.
25629918	0	28	theme	Nanostructural	0:13	arg1	morphology					15:24	Nanostructural morphology	0:24	Nanostructural morphology of plasticized wheat gluten and modified potato starch composites: relationship to mechanical and barrier properties.	0:142	Nanostructural morphology of plasticized wheat gluten and modified potato starch composites: relationship to mechanical and barrier properties.
25629918	7	29	from	extensibility	1318:1330	arg1	E-modulus					1364:1372	E-modulus	1364:1372	E-modulus	1364:1372	Highly polymerized WG protein was found in the samples processed at 130 °C versus 110 °C. Also, greater amounts of WG protein in the blend resulted in greater extensibility (110 °C) and a decrease in both E-modulus and maximum stress at 110 and 130 °C, respectively.
25629918	7	29	from	extensibility	1318:1330	arg1	stress					1386:1391	maximum stress	1378:1391	maximum stress	1378:1391	Highly polymerized WG protein was found in the samples processed at 130 °C versus 110 °C. Also, greater amounts of WG protein in the blend resulted in greater extensibility (110 °C) and a decrease in both E-modulus and maximum stress at 110 and 130 °C, respectively.
25629918	6	30	theme	β-sheets	1060:1067	arg1	amount					1050:1055	A larger amount	1041:1055	A larger amount of β-sheets	1041:1067	A larger amount of β-sheets was observed in the samples 70/30 and 30/70 WG-MPS processed at 130 °C with 45% glycerol.
25629918	6	30	theme	β-sheets	1060:1067	arg1	β-sheets					1060:1067	β-sheets	1060:1067	β-sheets	1060:1067	A larger amount of β-sheets was observed in the samples 70/30 and 30/70 WG-MPS processed at 130 °C with 45% glycerol.
25629918	2	31	theme	length	408:413	arg1	distribution					415:426	an altered chain length distribution	391:426	an altered chain length distribution of the amylopectin fraction and slightly increased amylose content compared to wild type potato starch	391:529	Characterization of the MPS revealed an altered chain length distribution of the amylopectin fraction and slightly increased amylose content compared to wild type potato starch.
25629918	2	32	theme	altered	394:400	arg1	distribution					415:426	an altered chain length distribution	391:426	an altered chain length distribution of the amylopectin fraction and slightly increased amylose content compared to wild type potato starch	391:529	Characterization of the MPS revealed an altered chain length distribution of the amylopectin fraction and slightly increased amylose content compared to wild type potato starch.
25629918	1	33	theme	potato	263:268	arg1	MPS					278:280	MPS	278:280	MPS	278:280	In the present study, we were able to produce composites of wheat gluten (WG) protein and a novel genetically modified potato starch (MPS) with attractive mechanical and gas barrier properties using extrusion.
25629918	1	33	theme	potato	263:268	arg1	starch					270:275	a novel genetically modified potato starch	234:275	a novel genetically modified potato starch (MPS) with attractive mechanical and gas barrier properties using extrusion	234:351	In the present study, we were able to produce composites of wheat gluten (WG) protein and a novel genetically modified potato starch (MPS) with attractive mechanical and gas barrier properties using extrusion.
25629918	6	34	dep	samples	1089:1095	arg1	70/30					1097:1101	70/30	1097:1101	70/30	1097:1101	A larger amount of β-sheets was observed in the samples 70/30 and 30/70 WG-MPS processed at 130 °C with 45% glycerol.
25629918	6	34	dep	samples	1089:1095	arg1	WG-MPS					1113:1118	30/70 WG-MPS	1107:1118	30/70 WG-MPS	1107:1118	A larger amount of β-sheets was observed in the samples 70/30 and 30/70 WG-MPS processed at 130 °C with 45% glycerol.
25629918	0	35	theme	wheat	41:45	arg1	composites					81:90	plasticized wheat gluten and modified potato starch composites	29:90	plasticized wheat gluten and modified potato starch composites	29:90	Nanostructural morphology of plasticized wheat gluten and modified potato starch composites: relationship to mechanical and barrier properties.
25629918	1	36	with	composites	190:199	arg1	properties					326:335	attractive mechanical and gas barrier properties	288:335	properties	326:335	In the present study, we were able to produce composites of wheat gluten (WG) protein and a novel genetically modified potato starch (MPS) with attractive mechanical and gas barrier properties using extrusion.
25629918	1	36	with	composites	190:199	arg1	mechanical					299:308	mechanical	299:308	mechanical	299:308	In the present study, we were able to produce composites of wheat gluten (WG) protein and a novel genetically modified potato starch (MPS) with attractive mechanical and gas barrier properties using extrusion.
25629918	5	37	theme	WG	1031:1032	arg1	films					1034:1038	WG films	1031:1038	WG films	1031:1038	WG has a structure resembling the hexagonal macromolecular arrangement as reported previously in WG films.
25629918	7	38	theme	greater	1255:1261	arg1	amounts					1263:1269	greater amounts	1255:1269	greater amounts of WG protein in the blend	1255:1296	Highly polymerized WG protein was found in the samples processed at 130 °C versus 110 °C. Also, greater amounts of WG protein in the blend resulted in greater extensibility (110 °C) and a decrease in both E-modulus and maximum stress at 110 and 130 °C, respectively.
25629918	7	38	theme	greater	1255:1261	arg1	protein					1277:1283	WG protein	1274:1283	WG protein in the blend	1274:1296	Highly polymerized WG protein was found in the samples processed at 130 °C versus 110 °C. Also, greater amounts of WG protein in the blend resulted in greater extensibility (110 °C) and a decrease in both E-modulus and maximum stress at 110 and 130 °C, respectively.
25629918	2	39	theme	amylopectin	435:445	arg1	fraction					447:454	the amylopectin fraction	431:454	the amylopectin fraction	431:454	Characterization of the MPS revealed an altered chain length distribution of the amylopectin fraction and slightly increased amylose content compared to wild type potato starch.
25629918	8	40	theme	45	1485:1486	arg1	%					1487:1487	%	1487:1487	%	1487:1487	Under ambient conditions the WG-MPS composite (70/30) with 45% glycerol showed excellent gas barrier properties to be further explored in multilayer film packaging applications.
25629918	7	41	from	amounts	1263:1269	arg1	blend					1292:1296	the blend	1288:1296	the blend	1288:1296	Highly polymerized WG protein was found in the samples processed at 130 °C versus 110 °C. Also, greater amounts of WG protein in the blend resulted in greater extensibility (110 °C) and a decrease in both E-modulus and maximum stress at 110 and 130 °C, respectively.
25629918	1	42	theme	gluten	210:215	arg1	WG					218:219	WG	218:219	WG	218:219	In the present study, we were able to produce composites of wheat gluten (WG) protein and a novel genetically modified potato starch (MPS) with attractive mechanical and gas barrier properties using extrusion.
25629918	1	42	theme	gluten	210:215	arg1	protein					222:228	wheat gluten (WG) protein	204:228	wheat gluten (WG) protein	204:228	In the present study, we were able to produce composites of wheat gluten (WG) protein and a novel genetically modified potato starch (MPS) with attractive mechanical and gas barrier properties using extrusion.
25629918	8	43	theme	excellent	1505:1513	arg1	properties					1527:1536	excellent gas barrier properties	1505:1536	excellent gas barrier properties	1505:1536	Under ambient conditions the WG-MPS composite (70/30) with 45% glycerol showed excellent gas barrier properties to be further explored in multilayer film packaging applications.
25629918	2	44	theme	MPS	378:380	arg1	Characterization					354:369	Characterization	354:369	Characterization of the MPS	354:380	Characterization of the MPS revealed an altered chain length distribution of the amylopectin fraction and slightly increased amylose content compared to wild type potato starch.
25629918	8	45	theme	film	1575:1578	arg1	applications					1590:1601	multilayer film packaging applications	1564:1601	multilayer film packaging applications	1564:1601	Under ambient conditions the WG-MPS composite (70/30) with 45% glycerol showed excellent gas barrier properties to be further explored in multilayer film packaging applications.
25629918	8	46	theme	barrier	1519:1525	arg1	properties					1527:1536	excellent gas barrier properties	1505:1536	excellent gas barrier properties	1505:1536	Under ambient conditions the WG-MPS composite (70/30) with 45% glycerol showed excellent gas barrier properties to be further explored in multilayer film packaging applications.
25629918	7	47	located	found	1193:1197	arg2	protein					1181:1187	Highly polymerized WG protein	1159:1187	Highly polymerized WG protein	1159:1187	Highly polymerized WG protein was found in the samples processed at 130 °C versus 110 °C. Also, greater amounts of WG protein in the blend resulted in greater extensibility (110 °C) and a decrease in both E-modulus and maximum stress at 110 and 130 °C, respectively.
25629918	7	47	located	found	1193:1197	arg1	samples					1206:1212	the samples	1202:1212	the samples processed at 130 °C versus 110 °C.	1202:1247	Highly polymerized WG protein was found in the samples processed at 130 °C versus 110 °C. Also, greater amounts of WG protein in the blend resulted in greater extensibility (110 °C) and a decrease in both E-modulus and maximum stress at 110 and 130 °C, respectively.
25629918	1	48	theme	protein	222:228	arg1	composites					190:199	composites	190:199	composites of wheat gluten (WG) protein	190:228	In the present study, we were able to produce composites of wheat gluten (WG) protein and a novel genetically modified potato starch (MPS) with attractive mechanical and gas barrier properties using extrusion.
25629918	1	48	theme	protein	222:228	arg1	starch					270:275	a novel genetically modified potato starch	234:275	a novel genetically modified potato starch (MPS) with attractive mechanical and gas barrier properties using extrusion	234:351	In the present study, we were able to produce composites of wheat gluten (WG) protein and a novel genetically modified potato starch (MPS) with attractive mechanical and gas barrier properties using extrusion.
25629918	1	48	theme	protein	222:228	arg1	protein					222:228	wheat gluten (WG) protein	204:228	wheat gluten (WG) protein	204:228	In the present study, we were able to produce composites of wheat gluten (WG) protein and a novel genetically modified potato starch (MPS) with attractive mechanical and gas barrier properties using extrusion.
25629918	1	48	theme	protein	222:228	arg1	MPS					278:280	MPS	278:280	MPS	278:280	In the present study, we were able to produce composites of wheat gluten (WG) protein and a novel genetically modified potato starch (MPS) with attractive mechanical and gas barrier properties using extrusion.
25629918	4	49	theme	B-type	859:864	arg1	structure					874:882	a B-type crystal structure	857:882	a B-type crystal structure observed by wide-angle X-ray scattering analysis	857:931	The nanomorphology of the composites showed the MPS having semicrystalline structure of a characteristic lamellar arrangement with an approximately 100 Å period observed by small-angle X-ray scattering and a B-type crystal structure observed by wide-angle X-ray scattering analysis.
25629918	7	50	theme	maximum	1378:1384	arg1	stress					1386:1391	maximum stress	1378:1391	maximum stress	1378:1391	Highly polymerized WG protein was found in the samples processed at 130 °C versus 110 °C. Also, greater amounts of WG protein in the blend resulted in greater extensibility (110 °C) and a decrease in both E-modulus and maximum stress at 110 and 130 °C, respectively.
25629918	6	51	theme	%	1147:1147	arg1	glycerol					1149:1156	45% glycerol	1145:1156	45% glycerol	1145:1156	A larger amount of β-sheets was observed in the samples 70/30 and 30/70 WG-MPS processed at 130 °C with 45% glycerol.
25629918	4	52	theme	X-ray	836:840	arg1	scattering					842:851	small-angle X-ray scattering	824:851	small-angle X-ray scattering	824:851	The nanomorphology of the composites showed the MPS having semicrystalline structure of a characteristic lamellar arrangement with an approximately 100 Å period observed by small-angle X-ray scattering and a B-type crystal structure observed by wide-angle X-ray scattering analysis.
25629918	4	53	theme	X-ray	907:911	arg1	analysis					924:931	wide-angle X-ray scattering analysis	896:931	wide-angle X-ray scattering analysis	896:931	The nanomorphology of the composites showed the MPS having semicrystalline structure of a characteristic lamellar arrangement with an approximately 100 Å period observed by small-angle X-ray scattering and a B-type crystal structure observed by wide-angle X-ray scattering analysis.
25629918	5	54	contain	has	937:939	arg1	WG					934:935	WG	934:935	WG	934:935	WG has a structure resembling the hexagonal macromolecular arrangement as reported previously in WG films.
25629918	5	54	contain	has	937:939	arg2	structure					943:951	a structure	941:951	a structure resembling the hexagonal macromolecular arrangement as reported previously in WG films	941:1038	WG has a structure resembling the hexagonal macromolecular arrangement as reported previously in WG films.
25629918	4	55	contain	having	703:708	arg1	MPS					699:701	the MPS	695:701	the MPS having semicrystalline structure of a characteristic lamellar arrangement with an approximately 100 Å period observed by small-angle X-ray scattering and a B-type crystal structure observed by wide-angle X-ray scattering analysis	695:931	The nanomorphology of the composites showed the MPS having semicrystalline structure of a characteristic lamellar arrangement with an approximately 100 Å period observed by small-angle X-ray scattering and a B-type crystal structure observed by wide-angle X-ray scattering analysis.
25629918	4	55	contain	having	703:708	arg2	structure					726:734	semicrystalline structure	710:734	semicrystalline structure of a characteristic lamellar arrangement	710:775	The nanomorphology of the composites showed the MPS having semicrystalline structure of a characteristic lamellar arrangement with an approximately 100 Å period observed by small-angle X-ray scattering and a B-type crystal structure observed by wide-angle X-ray scattering analysis.
25629918	4	56	dep	Å	803:803	arg1	100					799:801	100	799:801	100	799:801	The nanomorphology of the composites showed the MPS having semicrystalline structure of a characteristic lamellar arrangement with an approximately 100 Å period observed by small-angle X-ray scattering and a B-type crystal structure observed by wide-angle X-ray scattering analysis.
25629918	6	57	with	°C	1137:1138	arg1	glycerol					1149:1156	45% glycerol	1145:1156	45% glycerol	1145:1156	A larger amount of β-sheets was observed in the samples 70/30 and 30/70 WG-MPS processed at 130 °C with 45% glycerol.
25629918	2	58	theme	potato	517:522	arg1	starch					524:529	wild type potato starch	507:529	wild type potato starch	507:529	Characterization of the MPS revealed an altered chain length distribution of the amylopectin fraction and slightly increased amylose content compared to wild type potato starch.
25629918	3	59	theme	ratios	556:561	arg1	MPS					539:541	MPS	539:541	MPS	539:541	WG and MPS of different ratios plasticized with either glycerol or glycerol and water were extruded at 110 and 130 °C.
25629918	3	59	theme	ratios	556:561	arg1	WG					532:533	WG	532:533	WG	532:533	WG and MPS of different ratios plasticized with either glycerol or glycerol and water were extruded at 110 and 130 °C.
25629918	7	60	theme	greater	1310:1316	arg1	extensibility					1318:1330	greater extensibility	1310:1330	greater extensibility (110 °C)	1310:1339	Highly polymerized WG protein was found in the samples processed at 130 °C versus 110 °C. Also, greater amounts of WG protein in the blend resulted in greater extensibility (110 °C) and a decrease in both E-modulus and maximum stress at 110 and 130 °C, respectively.
25629918	7	60	theme	greater	1310:1316	arg1	°C					1337:1338	110 °C	1333:1338	110 °C	1333:1338	Highly polymerized WG protein was found in the samples processed at 130 °C versus 110 °C. Also, greater amounts of WG protein in the blend resulted in greater extensibility (110 °C) and a decrease in both E-modulus and maximum stress at 110 and 130 °C, respectively.
25629918	0	61	theme	starch	74:79	arg1	composites					81:90	plasticized wheat gluten and modified potato starch composites	29:90	plasticized wheat gluten and modified potato starch composites	29:90	Nanostructural morphology of plasticized wheat gluten and modified potato starch composites: relationship to mechanical and barrier properties.
25629918	7	62	from	decrease	1347:1354	arg1	E-modulus					1364:1372	E-modulus	1364:1372	E-modulus	1364:1372	Highly polymerized WG protein was found in the samples processed at 130 °C versus 110 °C. Also, greater amounts of WG protein in the blend resulted in greater extensibility (110 °C) and a decrease in both E-modulus and maximum stress at 110 and 130 °C, respectively.
25629918	7	62	from	decrease	1347:1354	arg1	stress					1386:1391	maximum stress	1378:1391	maximum stress	1378:1391	Highly polymerized WG protein was found in the samples processed at 130 °C versus 110 °C. Also, greater amounts of WG protein in the blend resulted in greater extensibility (110 °C) and a decrease in both E-modulus and maximum stress at 110 and 130 °C, respectively.
25629918	2	63	theme	amylose	479:485	arg1	content					487:493	slightly increased amylose content	460:493	slightly increased amylose content	460:493	Characterization of the MPS revealed an altered chain length distribution of the amylopectin fraction and slightly increased amylose content compared to wild type potato starch.
25629918	8	64	theme	ambient	1432:1438	arg1	conditions					1440:1449	ambient conditions	1432:1449	ambient conditions	1432:1449	Under ambient conditions the WG-MPS composite (70/30) with 45% glycerol showed excellent gas barrier properties to be further explored in multilayer film packaging applications.
25629918	1	65	theme	gas	314:316	arg1	properties					326:335	attractive mechanical and gas barrier properties	288:335	properties	326:335	In the present study, we were able to produce composites of wheat gluten (WG) protein and a novel genetically modified potato starch (MPS) with attractive mechanical and gas barrier properties using extrusion.
25629918	2	66	theme	wild	507:510	arg1	starch					524:529	wild type potato starch	507:529	wild type potato starch	507:529	Characterization of the MPS revealed an altered chain length distribution of the amylopectin fraction and slightly increased amylose content compared to wild type potato starch.
25629918	6	67	located	observed	1073:1080	arg2	β-sheets					1060:1067	β-sheets	1060:1067	β-sheets	1060:1067	A larger amount of β-sheets was observed in the samples 70/30 and 30/70 WG-MPS processed at 130 °C with 45% glycerol.
25629918	6	67	located	observed	1073:1080	arg1	samples					1089:1095	the samples 70/30 and 30/70 WG-MPS	1085:1118	the samples 70/30 and 30/70 WG-MPS processed at 130 °C with 45% glycerol	1085:1156	A larger amount of β-sheets was observed in the samples 70/30 and 30/70 WG-MPS processed at 130 °C with 45% glycerol.
25629918	6	67	located	observed	1073:1080	arg2	amount					1050:1055	A larger amount	1041:1055	A larger amount of β-sheets	1041:1067	A larger amount of β-sheets was observed in the samples 70/30 and 30/70 WG-MPS processed at 130 °C with 45% glycerol.
25629918	7	68	theme	polymerized	1166:1176	arg1	protein					1181:1187	Highly polymerized WG protein	1159:1187	Highly polymerized WG protein	1159:1187	Highly polymerized WG protein was found in the samples processed at 130 °C versus 110 °C. Also, greater amounts of WG protein in the blend resulted in greater extensibility (110 °C) and a decrease in both E-modulus and maximum stress at 110 and 130 °C, respectively.
25629918	5	69	theme	hexagonal	968:976	arg1	arrangement					993:1003	the hexagonal macromolecular arrangement	964:1003	the hexagonal macromolecular arrangement as reported previously in WG films	964:1038	WG has a structure resembling the hexagonal macromolecular arrangement as reported previously in WG films.
25629918	4	70	theme	characteristic	741:754	arg1	arrangement					765:775	a characteristic lamellar arrangement	739:775	a characteristic lamellar arrangement	739:775	The nanomorphology of the composites showed the MPS having semicrystalline structure of a characteristic lamellar arrangement with an approximately 100 Å period observed by small-angle X-ray scattering and a B-type crystal structure observed by wide-angle X-ray scattering analysis.
25629918	7	71	theme	WG	1274:1275	arg1	protein					1277:1283	WG protein	1274:1283	WG protein in the blend	1274:1296	Highly polymerized WG protein was found in the samples processed at 130 °C versus 110 °C. Also, greater amounts of WG protein in the blend resulted in greater extensibility (110 °C) and a decrease in both E-modulus and maximum stress at 110 and 130 °C, respectively.
25629918	0	72	theme	plasticized	29:39	arg1	composites					81:90	plasticized wheat gluten and modified potato starch composites	29:90	plasticized wheat gluten and modified potato starch composites	29:90	Nanostructural morphology of plasticized wheat gluten and modified potato starch composites: relationship to mechanical and barrier properties.
25629918	1	73	theme	modified	254:261	arg1	MPS					278:280	MPS	278:280	MPS	278:280	In the present study, we were able to produce composites of wheat gluten (WG) protein and a novel genetically modified potato starch (MPS) with attractive mechanical and gas barrier properties using extrusion.
25629918	1	73	theme	modified	254:261	arg1	starch					270:275	a novel genetically modified potato starch	234:275	a novel genetically modified potato starch (MPS) with attractive mechanical and gas barrier properties using extrusion	234:351	In the present study, we were able to produce composites of wheat gluten (WG) protein and a novel genetically modified potato starch (MPS) with attractive mechanical and gas barrier properties using extrusion.
25629918	2	74	theme	chain	402:406	arg1	distribution					415:426	an altered chain length distribution	391:426	an altered chain length distribution of the amylopectin fraction and slightly increased amylose content compared to wild type potato starch	391:529	Characterization of the MPS revealed an altered chain length distribution of the amylopectin fraction and slightly increased amylose content compared to wild type potato starch.
25629918	4	75	theme	semicrystalline	710:724	arg1	structure					726:734	semicrystalline structure	710:734	semicrystalline structure of a characteristic lamellar arrangement	710:775	The nanomorphology of the composites showed the MPS having semicrystalline structure of a characteristic lamellar arrangement with an approximately 100 Å period observed by small-angle X-ray scattering and a B-type crystal structure observed by wide-angle X-ray scattering analysis.
25629918	0	76	theme	gluten	47:52	arg1	composites					81:90	plasticized wheat gluten and modified potato starch composites	29:90	plasticized wheat gluten and modified potato starch composites	29:90	Nanostructural morphology of plasticized wheat gluten and modified potato starch composites: relationship to mechanical and barrier properties.
25629918	6	77	theme	larger	1043:1048	arg1	amount					1050:1055	A larger amount	1041:1055	A larger amount of β-sheets	1041:1067	A larger amount of β-sheets was observed in the samples 70/30 and 30/70 WG-MPS processed at 130 °C with 45% glycerol.
25629918	6	77	theme	larger	1043:1048	arg1	β-sheets					1060:1067	β-sheets	1060:1067	β-sheets	1060:1067	A larger amount of β-sheets was observed in the samples 70/30 and 30/70 WG-MPS processed at 130 °C with 45% glycerol.
25629918	2	78	theme	fraction	447:454	arg1	distribution					415:426	an altered chain length distribution	391:426	an altered chain length distribution of the amylopectin fraction and slightly increased amylose content compared to wild type potato starch	391:529	Characterization of the MPS revealed an altered chain length distribution of the amylopectin fraction and slightly increased amylose content compared to wild type potato starch.
25629918	8	79	theme	%	1487:1487	arg1	glycerol					1489:1496	45% glycerol	1485:1496	45% glycerol	1485:1496	Under ambient conditions the WG-MPS composite (70/30) with 45% glycerol showed excellent gas barrier properties to be further explored in multilayer film packaging applications.
25629918	7	80	theme	WG	1178:1179	arg1	protein					1181:1187	Highly polymerized WG protein	1159:1187	Highly polymerized WG protein	1159:1187	Highly polymerized WG protein was found in the samples processed at 130 °C versus 110 °C. Also, greater amounts of WG protein in the blend resulted in greater extensibility (110 °C) and a decrease in both E-modulus and maximum stress at 110 and 130 °C, respectively.
25629918	0	81	theme	modified	58:65	arg1	composites					81:90	plasticized wheat gluten and modified potato starch composites	29:90	plasticized wheat gluten and modified potato starch composites	29:90	Nanostructural morphology of plasticized wheat gluten and modified potato starch composites: relationship to mechanical and barrier properties.
25629918	8	82	theme	multilayer	1564:1573	arg1	applications					1590:1601	multilayer film packaging applications	1564:1601	multilayer film packaging applications	1564:1601	Under ambient conditions the WG-MPS composite (70/30) with 45% glycerol showed excellent gas barrier properties to be further explored in multilayer film packaging applications.
25629918	8	83	theme	gas	1515:1517	arg1	properties					1527:1536	excellent gas barrier properties	1505:1536	excellent gas barrier properties	1505:1536	Under ambient conditions the WG-MPS composite (70/30) with 45% glycerol showed excellent gas barrier properties to be further explored in multilayer film packaging applications.
25629918	4	84	theme	arrangement	765:775	arg1	structure					726:734	semicrystalline structure	710:734	semicrystalline structure of a characteristic lamellar arrangement	710:775	The nanomorphology of the composites showed the MPS having semicrystalline structure of a characteristic lamellar arrangement with an approximately 100 Å period observed by small-angle X-ray scattering and a B-type crystal structure observed by wide-angle X-ray scattering analysis.
25629918	8	85	theme	packaging	1580:1588	arg1	applications					1590:1601	multilayer film packaging applications	1564:1601	multilayer film packaging applications	1564:1601	Under ambient conditions the WG-MPS composite (70/30) with 45% glycerol showed excellent gas barrier properties to be further explored in multilayer film packaging applications.
25629918	0	86	dep	morphology	15:24	arg1	relationship					93:104	relationship	93:104	Nanostructural morphology of plasticized wheat gluten and modified potato starch composites: relationship to mechanical and barrier properties.	0:142	Nanostructural morphology of plasticized wheat gluten and modified potato starch composites: relationship to mechanical and barrier properties.
25629918	7	87	from	protein	1277:1283	arg1	blend					1292:1296	the blend	1288:1296	the blend	1288:1296	Highly polymerized WG protein was found in the samples processed at 130 °C versus 110 °C. Also, greater amounts of WG protein in the blend resulted in greater extensibility (110 °C) and a decrease in both E-modulus and maximum stress at 110 and 130 °C, respectively.
25630395	6	0	theme	NMR	974:976	arg1	studies					988:994	The NMR and GC-MS studies	970:994	The NMR and GC-MS studies	970:994	The NMR and GC-MS studies revealed that the most probable component for the cross-reaction is the rare sugar, colitose.
25630395	1	1	theme	Gram-negative	184:196	arg1	bacteria					198:205	Gram-negative bacteria	184:205	Gram-negative bacteria	184:205	The structure of the oligosaccharide repeating units of endotoxins from Gram-negative bacteria is characteristic for the different serogroups and serotypes of bacteria.
25630395	0	2	theme	serotypes	101:109	arg1	bacteria					63:70	bacteria	63:70	bacteria of different enterobacterial serotypes	63:109	Structural background for serological cross-reactivity between bacteria of different enterobacterial serotypes.
25630395	4	3	theme	bacteria	556:563	arg1	endotoxins					538:547	the endotoxins	534:547	the endotoxins of the bacteria	534:563	Fine differences between the endotoxins of the bacteria were detected using silver staining of SDS-PAGE gels and chip-technology for the intact lipopolysaccharides (LPSs).
25630395	2	4	theme	Escherichia	383:393	arg1	serotypes					350:358	three enterobacterial serotypes	328:358	three enterobacterial serotypes	328:358	Detailed examination of the cross-reactions of three enterobacterial serotypes, Proteus morganii O34, Escherichia coli O111, and Salmonella enterica sv.
25630395	2	4	theme	Escherichia	383:393	arg1	O111					400:403	Escherichia coli O111	383:403	Escherichia coli O111	383:403	Detailed examination of the cross-reactions of three enterobacterial serotypes, Proteus morganii O34, Escherichia coli O111, and Salmonella enterica sv.
25630395	5	5	theme	O-specific	705:714	arg1	polysaccharides					716:730	the O-specific polysaccharides	701:730	the O-specific polysaccharides	701:730	The compositions of the O-specific polysaccharides of LPSs extracted from the bacteria were studied, and it was proven that the three cross-reacting bacteria contain O-antigens built from the same monosaccharides, namely colitoses linked to glucose, galactose, and N-acetyl-galactosamine.
25630395	1	6	from	bacteria	198:205	arg1	units					159:163	the oligosaccharide repeating units	129:163	the oligosaccharide repeating units of endotoxins from Gram-negative bacteria	129:205	The structure of the oligosaccharide repeating units of endotoxins from Gram-negative bacteria is characteristic for the different serogroups and serotypes of bacteria.
25630395	1	6	from	bacteria	198:205	arg1	endotoxins					168:177	endotoxins	168:177	endotoxins from Gram-negative bacteria	168:205	The structure of the oligosaccharide repeating units of endotoxins from Gram-negative bacteria is characteristic for the different serogroups and serotypes of bacteria.
25630395	2	7	theme	serotypes	350:358	arg1	cross-reactions					309:323	the cross-reactions	305:323	the cross-reactions of three enterobacterial serotypes, Proteus morganii O34, Escherichia coli O111, and Salmonella enterica sv	305:431	Detailed examination of the cross-reactions of three enterobacterial serotypes, Proteus morganii O34, Escherichia coli O111, and Salmonella enterica sv.
25630395	5	8	theme	polysaccharides	716:730	arg1	compositions					685:696	The compositions	681:696	The compositions of the O-specific polysaccharides of LPSs extracted from the bacteria	681:766	The compositions of the O-specific polysaccharides of LPSs extracted from the bacteria were studied, and it was proven that the three cross-reacting bacteria contain O-antigens built from the same monosaccharides, namely colitoses linked to glucose, galactose, and N-acetyl-galactosamine.
25630395	2	9	theme	enterobacterial	334:348	arg1	sv					430:431	Salmonella enterica sv	410:431	Salmonella enterica sv	410:431	Detailed examination of the cross-reactions of three enterobacterial serotypes, Proteus morganii O34, Escherichia coli O111, and Salmonella enterica sv.
25630395	2	9	theme	enterobacterial	334:348	arg1	serotypes					350:358	three enterobacterial serotypes	328:358	three enterobacterial serotypes	328:358	Detailed examination of the cross-reactions of three enterobacterial serotypes, Proteus morganii O34, Escherichia coli O111, and Salmonella enterica sv.
25630395	2	9	theme	enterobacterial	334:348	arg1	O111					400:403	Escherichia coli O111	383:403	Escherichia coli O111	383:403	Detailed examination of the cross-reactions of three enterobacterial serotypes, Proteus morganii O34, Escherichia coli O111, and Salmonella enterica sv.
25630395	2	9	theme	enterobacterial	334:348	arg1	O34					378:380	Proteus morganii O34	361:380	Proteus morganii O34	361:380	Detailed examination of the cross-reactions of three enterobacterial serotypes, Proteus morganii O34, Escherichia coli O111, and Salmonella enterica sv.
25630395	4	10	theme	Fine	509:512	arg1	differences					514:524	Fine differences	509:524	Fine differences between the endotoxins of the bacteria	509:563	Fine differences between the endotoxins of the bacteria were detected using silver staining of SDS-PAGE gels and chip-technology for the intact lipopolysaccharides (LPSs).
25630395	1	11	from	units	159:163	arg1	bacteria					198:205	Gram-negative bacteria	184:205	Gram-negative bacteria	184:205	The structure of the oligosaccharide repeating units of endotoxins from Gram-negative bacteria is characteristic for the different serogroups and serotypes of bacteria.
25630395	2	12	dep	Salmonella	410:419	arg1	enterica					421:428	enterica	421:428	enterica	421:428	Detailed examination of the cross-reactions of three enterobacterial serotypes, Proteus morganii O34, Escherichia coli O111, and Salmonella enterica sv.
25630395	3	13	theme	Adelaide	434:441	arg1	O35					443:445	Adelaide O35	434:445	Adelaide O35	434:445	Adelaide O35, was performed using sensitive tests (ELISA, immunoblotting).
25630395	2	14	dep	Escherichia	383:393	arg1	coli					395:398	coli	395:398	coli	395:398	Detailed examination of the cross-reactions of three enterobacterial serotypes, Proteus morganii O34, Escherichia coli O111, and Salmonella enterica sv.
25630395	3	15	theme	sensitive	468:476	arg1	tests					478:482	sensitive tests	468:482	sensitive tests (ELISA, immunoblotting)	468:506	Adelaide O35, was performed using sensitive tests (ELISA, immunoblotting).
25630395	0	16	theme	Structural	0:9	arg1	background					11:20	Structural background	0:20	Structural background for serological cross-reactivity between bacteria of different enterobacterial serotypes.	0:110	Structural background for serological cross-reactivity between bacteria of different enterobacterial serotypes.
25630395	2	17	theme	morganii	369:376	arg1	serotypes					350:358	three enterobacterial serotypes	328:358	three enterobacterial serotypes	328:358	Detailed examination of the cross-reactions of three enterobacterial serotypes, Proteus morganii O34, Escherichia coli O111, and Salmonella enterica sv.
25630395	2	17	theme	morganii	369:376	arg1	O34					378:380	Proteus morganii O34	361:380	Proteus morganii O34	361:380	Detailed examination of the cross-reactions of three enterobacterial serotypes, Proteus morganii O34, Escherichia coli O111, and Salmonella enterica sv.
25630395	0	18	theme	serological	26:36	arg1	cross-reactivity					38:53	serological cross-reactivity	26:53	serological cross-reactivity	26:53	Structural background for serological cross-reactivity between bacteria of different enterobacterial serotypes.
25630395	6	19	theme	probable	1019:1026	arg1	sugar					1073:1077	the rare sugar	1064:1077	the rare sugar	1064:1077	The NMR and GC-MS studies revealed that the most probable component for the cross-reaction is the rare sugar, colitose.
25630395	6	19	theme	probable	1019:1026	arg1	component					1028:1036	the most probable component	1010:1036	the most probable component for the cross-reaction	1010:1059	The NMR and GC-MS studies revealed that the most probable component for the cross-reaction is the rare sugar, colitose.
25630395	2	20	theme	Proteus	361:367	arg1	serotypes					350:358	three enterobacterial serotypes	328:358	three enterobacterial serotypes	328:358	Detailed examination of the cross-reactions of three enterobacterial serotypes, Proteus morganii O34, Escherichia coli O111, and Salmonella enterica sv.
25630395	2	20	theme	Proteus	361:367	arg1	O34					378:380	Proteus morganii O34	361:380	Proteus morganii O34	361:380	Detailed examination of the cross-reactions of three enterobacterial serotypes, Proteus morganii O34, Escherichia coli O111, and Salmonella enterica sv.
25630395	1	21	theme	different	233:241	arg1	serogroups					243:252	serogroups	243:252	serogroups	243:252	The structure of the oligosaccharide repeating units of endotoxins from Gram-negative bacteria is characteristic for the different serogroups and serotypes of bacteria.
25630395	5	22	theme	same	873:876	arg1	monosaccharides					878:892	the same monosaccharides	869:892	the same monosaccharides	869:892	The compositions of the O-specific polysaccharides of LPSs extracted from the bacteria were studied, and it was proven that the three cross-reacting bacteria contain O-antigens built from the same monosaccharides, namely colitoses linked to glucose, galactose, and N-acetyl-galactosamine.
25630395	5	22	theme	same	873:876	arg1	colitoses					902:910	colitoses	902:910	colitoses linked to glucose, galactose, and N-acetyl-galactosamine	902:967	The compositions of the O-specific polysaccharides of LPSs extracted from the bacteria were studied, and it was proven that the three cross-reacting bacteria contain O-antigens built from the same monosaccharides, namely colitoses linked to glucose, galactose, and N-acetyl-galactosamine.
25630395	4	23	theme	gels	613:616	arg1	chip-technology					622:636	chip-technology	622:636	chip-technology for the intact lipopolysaccharides (LPSs)	622:678	Fine differences between the endotoxins of the bacteria were detected using silver staining of SDS-PAGE gels and chip-technology for the intact lipopolysaccharides (LPSs).
25630395	4	23	theme	gels	613:616	arg1	staining					592:599	silver staining	585:599	silver staining of SDS-PAGE gels	585:616	Fine differences between the endotoxins of the bacteria were detected using silver staining of SDS-PAGE gels and chip-technology for the intact lipopolysaccharides (LPSs).
25630395	5	24	theme	cross-reacting	815:828	arg1	bacteria					830:837	the three cross-reacting bacteria	805:837	the three cross-reacting bacteria	805:837	The compositions of the O-specific polysaccharides of LPSs extracted from the bacteria were studied, and it was proven that the three cross-reacting bacteria contain O-antigens built from the same monosaccharides, namely colitoses linked to glucose, galactose, and N-acetyl-galactosamine.
25630395	4	25	theme	SDS-PAGE	604:611	arg1	gels					613:616	SDS-PAGE gels	604:616	SDS-PAGE gels	604:616	Fine differences between the endotoxins of the bacteria were detected using silver staining of SDS-PAGE gels and chip-technology for the intact lipopolysaccharides (LPSs).
25630395	5	26	attach	linked	912:917	arg1	glucose					922:928	glucose	922:928	glucose	922:928	The compositions of the O-specific polysaccharides of LPSs extracted from the bacteria were studied, and it was proven that the three cross-reacting bacteria contain O-antigens built from the same monosaccharides, namely colitoses linked to glucose, galactose, and N-acetyl-galactosamine.
25630395	5	26	attach	linked	912:917	arg1	galactose					931:939	galactose	931:939	galactose	931:939	The compositions of the O-specific polysaccharides of LPSs extracted from the bacteria were studied, and it was proven that the three cross-reacting bacteria contain O-antigens built from the same monosaccharides, namely colitoses linked to glucose, galactose, and N-acetyl-galactosamine.
25630395	5	26	attach	linked	912:917	arg1	N-acetyl-galactosamine					946:967	N-acetyl-galactosamine	946:967	N-acetyl-galactosamine	946:967	The compositions of the O-specific polysaccharides of LPSs extracted from the bacteria were studied, and it was proven that the three cross-reacting bacteria contain O-antigens built from the same monosaccharides, namely colitoses linked to glucose, galactose, and N-acetyl-galactosamine.
25630395	5	26	attach	linked	912:917	arg2	colitoses					902:910	colitoses	902:910	colitoses linked to glucose, galactose, and N-acetyl-galactosamine	902:967	The compositions of the O-specific polysaccharides of LPSs extracted from the bacteria were studied, and it was proven that the three cross-reacting bacteria contain O-antigens built from the same monosaccharides, namely colitoses linked to glucose, galactose, and N-acetyl-galactosamine.
25630395	5	26	attach	linked	912:917	arg2	monosaccharides					878:892	the same monosaccharides	869:892	the same monosaccharides	869:892	The compositions of the O-specific polysaccharides of LPSs extracted from the bacteria were studied, and it was proven that the three cross-reacting bacteria contain O-antigens built from the same monosaccharides, namely colitoses linked to glucose, galactose, and N-acetyl-galactosamine.
25630395	2	27	theme	Detailed	281:288	arg1	examination					290:300	Detailed examination	281:300	Detailed examination of the cross-reactions of three enterobacterial serotypes, Proteus morganii O34, Escherichia coli O111, and Salmonella enterica sv.	281:432	Detailed examination of the cross-reactions of three enterobacterial serotypes, Proteus morganii O34, Escherichia coli O111, and Salmonella enterica sv.
25630395	3	28	dep	tests	478:482	arg1	immunoblotting					492:505	immunoblotting	492:505	immunoblotting	492:505	Adelaide O35, was performed using sensitive tests (ELISA, immunoblotting).
25630395	3	28	dep	tests	478:482	arg1	ELISA					485:489	ELISA	485:489	ELISA	485:489	Adelaide O35, was performed using sensitive tests (ELISA, immunoblotting).
25630395	1	29	theme	oligosaccharide	133:147	arg1	units					159:163	the oligosaccharide repeating units	129:163	the oligosaccharide repeating units of endotoxins from Gram-negative bacteria	129:205	The structure of the oligosaccharide repeating units of endotoxins from Gram-negative bacteria is characteristic for the different serogroups and serotypes of bacteria.
25630395	4	30	theme	intact	646:651	arg1	lipopolysaccharides					653:671	the intact lipopolysaccharides	642:671	the intact lipopolysaccharides (LPSs)	642:678	Fine differences between the endotoxins of the bacteria were detected using silver staining of SDS-PAGE gels and chip-technology for the intact lipopolysaccharides (LPSs).
25630395	4	30	theme	intact	646:651	arg1	LPSs					674:677	LPSs	674:677	LPSs	674:677	Fine differences between the endotoxins of the bacteria were detected using silver staining of SDS-PAGE gels and chip-technology for the intact lipopolysaccharides (LPSs).
25630395	4	31	theme	silver	585:590	arg1	staining					592:599	silver staining	585:599	silver staining of SDS-PAGE gels	585:616	Fine differences between the endotoxins of the bacteria were detected using silver staining of SDS-PAGE gels and chip-technology for the intact lipopolysaccharides (LPSs).
25630395	5	32	theme	LPSs	735:738	arg1	compositions					685:696	The compositions	681:696	The compositions of the O-specific polysaccharides of LPSs extracted from the bacteria	681:766	The compositions of the O-specific polysaccharides of LPSs extracted from the bacteria were studied, and it was proven that the three cross-reacting bacteria contain O-antigens built from the same monosaccharides, namely colitoses linked to glucose, galactose, and N-acetyl-galactosamine.
25630395	1	33	theme	repeating	149:157	arg1	units					159:163	the oligosaccharide repeating units	129:163	the oligosaccharide repeating units of endotoxins from Gram-negative bacteria	129:205	The structure of the oligosaccharide repeating units of endotoxins from Gram-negative bacteria is characteristic for the different serogroups and serotypes of bacteria.
25630395	6	34	theme	GC-MS	982:986	arg1	studies					988:994	The NMR and GC-MS studies	970:994	The NMR and GC-MS studies	970:994	The NMR and GC-MS studies revealed that the most probable component for the cross-reaction is the rare sugar, colitose.
25630395	5	35	contain	contain	839:845	arg2	O-antigens					847:856	O-antigens	847:856	O-antigens built from the same monosaccharides, namely colitoses linked to glucose, galactose, and N-acetyl-galactosamine	847:967	The compositions of the O-specific polysaccharides of LPSs extracted from the bacteria were studied, and it was proven that the three cross-reacting bacteria contain O-antigens built from the same monosaccharides, namely colitoses linked to glucose, galactose, and N-acetyl-galactosamine.
25630395	5	35	contain	contain	839:845	arg1	bacteria					830:837	the three cross-reacting bacteria	805:837	the three cross-reacting bacteria	805:837	The compositions of the O-specific polysaccharides of LPSs extracted from the bacteria were studied, and it was proven that the three cross-reacting bacteria contain O-antigens built from the same monosaccharides, namely colitoses linked to glucose, galactose, and N-acetyl-galactosamine.
25630395	2	36	theme	cross-reactions	309:323	arg1	examination					290:300	Detailed examination	281:300	Detailed examination of the cross-reactions of three enterobacterial serotypes, Proteus morganii O34, Escherichia coli O111, and Salmonella enterica sv.	281:432	Detailed examination of the cross-reactions of three enterobacterial serotypes, Proteus morganii O34, Escherichia coli O111, and Salmonella enterica sv.
25630395	1	37	theme	units	159:163	arg1	structure					116:124	The structure	112:124	The structure of the oligosaccharide repeating units of endotoxins from Gram-negative bacteria	112:205	The structure of the oligosaccharide repeating units of endotoxins from Gram-negative bacteria is characteristic for the different serogroups and serotypes of bacteria.
25630395	1	37	theme	units	159:163	arg1	characteristic					210:223	characteristic	210:223	characteristic for the different serogroups and serotypes of bacteria	210:278	The structure of the oligosaccharide repeating units of endotoxins from Gram-negative bacteria is characteristic for the different serogroups and serotypes of bacteria.
25630395	1	38	theme	bacteria	271:278	arg1	serogroups					243:252	serogroups	243:252	serogroups	243:252	The structure of the oligosaccharide repeating units of endotoxins from Gram-negative bacteria is characteristic for the different serogroups and serotypes of bacteria.
25630395	1	38	theme	bacteria	271:278	arg1	serotypes					258:266	serotypes	258:266	serotypes	258:266	The structure of the oligosaccharide repeating units of endotoxins from Gram-negative bacteria is characteristic for the different serogroups and serotypes of bacteria.
25630395	0	39	theme	enterobacterial	85:99	arg1	serotypes					101:109	different enterobacterial serotypes	75:109	different enterobacterial serotypes	75:109	Structural background for serological cross-reactivity between bacteria of different enterobacterial serotypes.
25630395	1	40	theme	endotoxins	168:177	arg1	units					159:163	the oligosaccharide repeating units	129:163	the oligosaccharide repeating units of endotoxins from Gram-negative bacteria	129:205	The structure of the oligosaccharide repeating units of endotoxins from Gram-negative bacteria is characteristic for the different serogroups and serotypes of bacteria.
25630395	6	41	theme	rare	1068:1071	arg1	colitose					1080:1087	colitose	1080:1087	colitose	1080:1087	The NMR and GC-MS studies revealed that the most probable component for the cross-reaction is the rare sugar, colitose.
25630395	6	41	theme	rare	1068:1071	arg1	sugar					1073:1077	the rare sugar	1064:1077	the rare sugar	1064:1077	The NMR and GC-MS studies revealed that the most probable component for the cross-reaction is the rare sugar, colitose.
25630395	6	41	theme	rare	1068:1071	arg1	component					1028:1036	the most probable component	1010:1036	the most probable component for the cross-reaction	1010:1059	The NMR and GC-MS studies revealed that the most probable component for the cross-reaction is the rare sugar, colitose.
25630395	0	42	theme	different	75:83	arg1	serotypes					101:109	different enterobacterial serotypes	75:109	different enterobacterial serotypes	75:109	Structural background for serological cross-reactivity between bacteria of different enterobacterial serotypes.
25630395	1	43	dep	serogroups	243:252	arg1	the					229:231	the	229:231	the	229:231	The structure of the oligosaccharide repeating units of endotoxins from Gram-negative bacteria is characteristic for the different serogroups and serotypes of bacteria.
25630395	2	44	theme	Salmonella	410:419	arg1	sv					430:431	Salmonella enterica sv	410:431	Salmonella enterica sv	410:431	Detailed examination of the cross-reactions of three enterobacterial serotypes, Proteus morganii O34, Escherichia coli O111, and Salmonella enterica sv.
25630395	2	44	theme	Salmonella	410:419	arg1	serotypes					350:358	three enterobacterial serotypes	328:358	three enterobacterial serotypes	328:358	Detailed examination of the cross-reactions of three enterobacterial serotypes, Proteus morganii O34, Escherichia coli O111, and Salmonella enterica sv.
25649385	0	0	theme	CWB2	75:78	arg1	motifs					80:85	CWB2 motifs	75:85	CWB2 motifs that recognise the anionic polymer PSII	75:125	Clostridium difficile surface proteins are anchored to the cell wall using CWB2 motifs that recognise the anionic polymer PSII.
25649385	2	1	theme	proteins	469:476	arg1	number					449:454	a large number	441:454	a large number of cell wall proteins in the Firmicutes	441:494	We describe here a mechanism of protein anchoring involving tandem CWB2 motifs found in a large number of cell wall proteins in the Firmicutes.
25649385	7	2	from	mechanism	1305:1313	arg1	bacteria					1353:1360	Gram-positive bacteria	1339:1360	Gram-positive bacteria	1339:1360	We show that the anionic polymer PSII binds two cell wall proteins, SlpA and Cwp2, and these interactions require the CWB2 repeats, defining a new mechanism of protein anchoring in Gram-positive bacteria.
25649385	4	3	theme	CWB2	657:660	arg1	repeats					662:668	CWB2 repeats	657:668	CWB2 repeats	657:668	CWB2 repeats are non-identical and cannot substitute for each other, as shown by the secretion into the culture supernatant of proteins containing variations in the patterns of repeats.
25649385	1	4	theme	important	236:244	arg1	properties					246:255	important properties	236:255	important properties	236:255	Gram-positive surface proteins can be covalently or non-covalently anchored to the cell wall and can impart important properties on the bacterium in respect of cell envelope organisation and interaction with the environment.
25649385	2	5	theme	wall	464:467	arg1	proteins					469:476	cell wall proteins	459:476	cell wall proteins in the Firmicutes	459:494	We describe here a mechanism of protein anchoring involving tandem CWB2 motifs found in a large number of cell wall proteins in the Firmicutes.
25649385	3	6	theme	cell	646:649	arg1	wall					651:654	the cell wall	642:654	the cell wall	642:654	In the Clostridium difficile cell wall protein family, we show the three tandem repeats of the CWB2 motif are essential for correct anchoring to the cell wall.
25649385	1	7	with	organisation	302:313	arg1	environment					340:350	the environment	336:350	the environment	336:350	Gram-positive surface proteins can be covalently or non-covalently anchored to the cell wall and can impart important properties on the bacterium in respect of cell envelope organisation and interaction with the environment.
25649385	5	8	theme	Ile	855:857	arg1	sequence					867:874	A conserved Ile Leu Leu sequence	843:874	A conserved Ile Leu Leu sequence within the CWB2 repeats	843:898	A conserved Ile Leu Leu sequence within the CWB2 repeats is essential for correct anchoring, although a preceding proline residue is dispensable.
25649385	5	8	theme	Ile	855:857	arg1	essential					903:911	essential	903:911	essential	903:911	A conserved Ile Leu Leu sequence within the CWB2 repeats is essential for correct anchoring, although a preceding proline residue is dispensable.
25649385	1	9	with	interaction	319:329	arg1	environment					340:350	the environment	336:350	the environment	336:350	Gram-positive surface proteins can be covalently or non-covalently anchored to the cell wall and can impart important properties on the bacterium in respect of cell envelope organisation and interaction with the environment.
25649385	4	10	theme	repeats	834:840	arg1	patterns					822:829	the patterns	818:829	the patterns of repeats	818:840	CWB2 repeats are non-identical and cannot substitute for each other, as shown by the secretion into the culture supernatant of proteins containing variations in the patterns of repeats.
25649385	3	11	theme	cell	526:529	arg1	family					544:549	the Clostridium difficile cell wall protein family	500:549	the Clostridium difficile cell wall protein family	500:549	In the Clostridium difficile cell wall protein family, we show the three tandem repeats of the CWB2 motif are essential for correct anchoring to the cell wall.
25649385	7	12	theme	new	1301:1303	arg1	mechanism					1305:1313	a new mechanism	1299:1313	a new mechanism of protein anchoring in Gram-positive bacteria	1299:1360	We show that the anionic polymer PSII binds two cell wall proteins, SlpA and Cwp2, and these interactions require the CWB2 repeats, defining a new mechanism of protein anchoring in Gram-positive bacteria.
25649385	2	13	from	number	449:454	arg1	Firmicutes					485:494	the Firmicutes	481:494	the Firmicutes	481:494	We describe here a mechanism of protein anchoring involving tandem CWB2 motifs found in a large number of cell wall proteins in the Firmicutes.
25649385	3	14	theme	wall	531:534	arg1	family					544:549	the Clostridium difficile cell wall protein family	500:549	the Clostridium difficile cell wall protein family	500:549	In the Clostridium difficile cell wall protein family, we show the three tandem repeats of the CWB2 motif are essential for correct anchoring to the cell wall.
25649385	4	15	from	variations	804:813	arg1	patterns					822:829	the patterns	818:829	the patterns of repeats	818:840	CWB2 repeats are non-identical and cannot substitute for each other, as shown by the secretion into the culture supernatant of proteins containing variations in the patterns of repeats.
25649385	3	16	theme	Clostridium	504:514	arg1	family					544:549	the Clostridium difficile cell wall protein family	500:549	the Clostridium difficile cell wall protein family	500:549	In the Clostridium difficile cell wall protein family, we show the three tandem repeats of the CWB2 motif are essential for correct anchoring to the cell wall.
25649385	2	17	theme	protein	385:391	arg1	anchoring					393:401	protein anchoring	385:401	protein anchoring	385:401	We describe here a mechanism of protein anchoring involving tandem CWB2 motifs found in a large number of cell wall proteins in the Firmicutes.
25649385	7	18	from	bacteria	1353:1360	arg1	mechanism					1305:1313	a new mechanism	1299:1313	a new mechanism of protein anchoring in Gram-positive bacteria	1299:1360	We show that the anionic polymer PSII binds two cell wall proteins, SlpA and Cwp2, and these interactions require the CWB2 repeats, defining a new mechanism of protein anchoring in Gram-positive bacteria.
25649385	7	19	theme	Gram-positive	1339:1351	arg1	bacteria					1353:1360	Gram-positive bacteria	1339:1360	Gram-positive bacteria	1339:1360	We show that the anionic polymer PSII binds two cell wall proteins, SlpA and Cwp2, and these interactions require the CWB2 repeats, defining a new mechanism of protein anchoring in Gram-positive bacteria.
25649385	4	20	theme	proteins	784:791	arg1	supernatant					769:779	the culture supernatant	757:779	the culture supernatant of proteins containing variations in the patterns of repeats	757:840	CWB2 repeats are non-identical and cannot substitute for each other, as shown by the secretion into the culture supernatant of proteins containing variations in the patterns of repeats.
25649385	3	21	theme	difficile	516:524	arg1	family					544:549	the Clostridium difficile cell wall protein family	500:549	the Clostridium difficile cell wall protein family	500:549	In the Clostridium difficile cell wall protein family, we show the three tandem repeats of the CWB2 motif are essential for correct anchoring to the cell wall.
25649385	0	22	theme	difficile	12:20	arg1	proteins					30:37	Clostridium difficile surface proteins	0:37	Clostridium difficile surface proteins	0:37	Clostridium difficile surface proteins are anchored to the cell wall using CWB2 motifs that recognise the anionic polymer PSII.
25649385	7	23	theme	anionic	1175:1181	arg1	PSII					1191:1194	the anionic polymer PSII	1171:1194	the anionic polymer PSII	1171:1194	We show that the anionic polymer PSII binds two cell wall proteins, SlpA and Cwp2, and these interactions require the CWB2 repeats, defining a new mechanism of protein anchoring in Gram-positive bacteria.
25649385	7	24	theme	protein	1318:1324	arg1	anchoring					1326:1334	protein anchoring	1318:1334	protein anchoring in Gram-positive bacteria	1318:1360	We show that the anionic polymer PSII binds two cell wall proteins, SlpA and Cwp2, and these interactions require the CWB2 repeats, defining a new mechanism of protein anchoring in Gram-positive bacteria.
25649385	6	25	theme	genetic	1009:1015	arg1	locus					1017:1021	a likely genetic locus	1000:1021	a likely genetic locus encoding synthesis of the anionic polymer PSII	1000:1068	We propose a likely genetic locus encoding synthesis of the anionic polymer PSII and, using RNA knock-down of key genes, reveal subtle effects on cell wall composition.
25649385	0	26	theme	Clostridium	0:10	arg1	proteins					30:37	Clostridium difficile surface proteins	0:37	Clostridium difficile surface proteins	0:37	Clostridium difficile surface proteins are anchored to the cell wall using CWB2 motifs that recognise the anionic polymer PSII.
25649385	7	27	theme	anchoring	1326:1334	arg1	mechanism					1305:1313	a new mechanism	1299:1313	a new mechanism of protein anchoring in Gram-positive bacteria	1299:1360	We show that the anionic polymer PSII binds two cell wall proteins, SlpA and Cwp2, and these interactions require the CWB2 repeats, defining a new mechanism of protein anchoring in Gram-positive bacteria.
25649385	5	28	theme	CWB2	887:890	arg1	repeats					892:898	the CWB2 repeats	883:898	the CWB2 repeats	883:898	A conserved Ile Leu Leu sequence within the CWB2 repeats is essential for correct anchoring, although a preceding proline residue is dispensable.
25649385	6	29	theme	likely	1002:1007	arg1	locus					1017:1021	a likely genetic locus	1000:1021	a likely genetic locus encoding synthesis of the anionic polymer PSII	1000:1068	We propose a likely genetic locus encoding synthesis of the anionic polymer PSII and, using RNA knock-down of key genes, reveal subtle effects on cell wall composition.
25649385	0	30	theme	polymer	114:120	arg1	PSII					122:125	the anionic polymer PSII	102:125	the anionic polymer PSII	102:125	Clostridium difficile surface proteins are anchored to the cell wall using CWB2 motifs that recognise the anionic polymer PSII.
25649385	2	31	located	found	432:436	arg1	number					449:454	a large number	441:454	a large number of cell wall proteins in the Firmicutes	441:494	We describe here a mechanism of protein anchoring involving tandem CWB2 motifs found in a large number of cell wall proteins in the Firmicutes.
25649385	2	31	located	found	432:436	arg2	motifs					425:430	tandem CWB2 motifs	413:430	tandem CWB2 motifs found in a large number of cell wall proteins in the Firmicutes	413:494	We describe here a mechanism of protein anchoring involving tandem CWB2 motifs found in a large number of cell wall proteins in the Firmicutes.
25649385	3	32	theme	protein	536:542	arg1	family					544:549	the Clostridium difficile cell wall protein family	500:549	the Clostridium difficile cell wall protein family	500:549	In the Clostridium difficile cell wall protein family, we show the three tandem repeats of the CWB2 motif are essential for correct anchoring to the cell wall.
25649385	5	33	theme	proline	957:963	arg1	residue					965:971	a preceding proline residue	945:971	a preceding proline residue	945:971	A conserved Ile Leu Leu sequence within the CWB2 repeats is essential for correct anchoring, although a preceding proline residue is dispensable.
25649385	5	33	theme	proline	957:963	arg1	dispensable					976:986	dispensable	976:986	dispensable	976:986	A conserved Ile Leu Leu sequence within the CWB2 repeats is essential for correct anchoring, although a preceding proline residue is dispensable.
25649385	2	34	theme	CWB2	420:423	arg1	motifs					425:430	tandem CWB2 motifs	413:430	tandem CWB2 motifs found in a large number of cell wall proteins in the Firmicutes	413:494	We describe here a mechanism of protein anchoring involving tandem CWB2 motifs found in a large number of cell wall proteins in the Firmicutes.
25649385	0	35	theme	surface	22:28	arg1	proteins					30:37	Clostridium difficile surface proteins	0:37	Clostridium difficile surface proteins	0:37	Clostridium difficile surface proteins are anchored to the cell wall using CWB2 motifs that recognise the anionic polymer PSII.
25649385	0	36	theme	anionic	106:112	arg1	PSII					122:125	the anionic polymer PSII	102:125	the anionic polymer PSII	102:125	Clostridium difficile surface proteins are anchored to the cell wall using CWB2 motifs that recognise the anionic polymer PSII.
25649385	2	37	from	proteins	469:476	arg1	Firmicutes					485:494	the Firmicutes	481:494	the Firmicutes	481:494	We describe here a mechanism of protein anchoring involving tandem CWB2 motifs found in a large number of cell wall proteins in the Firmicutes.
25649385	2	38	theme	tandem	413:418	arg1	motifs					425:430	tandem CWB2 motifs	413:430	tandem CWB2 motifs found in a large number of cell wall proteins in the Firmicutes	413:494	We describe here a mechanism of protein anchoring involving tandem CWB2 motifs found in a large number of cell wall proteins in the Firmicutes.
25649385	2	39	theme	cell	459:462	arg1	proteins					469:476	cell wall proteins	459:476	cell wall proteins in the Firmicutes	459:494	We describe here a mechanism of protein anchoring involving tandem CWB2 motifs found in a large number of cell wall proteins in the Firmicutes.
25649385	7	40	theme	wall	1211:1214	arg1	proteins					1216:1223	two cell wall proteins	1202:1223	two cell wall proteins	1202:1223	We show that the anionic polymer PSII binds two cell wall proteins, SlpA and Cwp2, and these interactions require the CWB2 repeats, defining a new mechanism of protein anchoring in Gram-positive bacteria.
25649385	7	40	theme	wall	1211:1214	arg1	Cwp2					1235:1238	Cwp2	1235:1238	Cwp2	1235:1238	We show that the anionic polymer PSII binds two cell wall proteins, SlpA and Cwp2, and these interactions require the CWB2 repeats, defining a new mechanism of protein anchoring in Gram-positive bacteria.
25649385	7	40	theme	wall	1211:1214	arg1	SlpA					1226:1229	SlpA	1226:1229	SlpA	1226:1229	We show that the anionic polymer PSII binds two cell wall proteins, SlpA and Cwp2, and these interactions require the CWB2 repeats, defining a new mechanism of protein anchoring in Gram-positive bacteria.
25649385	7	41	from	anchoring	1326:1334	arg1	bacteria					1353:1360	Gram-positive bacteria	1339:1360	Gram-positive bacteria	1339:1360	We show that the anionic polymer PSII binds two cell wall proteins, SlpA and Cwp2, and these interactions require the CWB2 repeats, defining a new mechanism of protein anchoring in Gram-positive bacteria.
25649385	4	42	theme	culture	761:767	arg1	supernatant					769:779	the culture supernatant	757:779	the culture supernatant of proteins containing variations in the patterns of repeats	757:840	CWB2 repeats are non-identical and cannot substitute for each other, as shown by the secretion into the culture supernatant of proteins containing variations in the patterns of repeats.
25649385	5	43	theme	conserved	845:853	arg1	sequence					867:874	A conserved Ile Leu Leu sequence	843:874	A conserved Ile Leu Leu sequence within the CWB2 repeats	843:898	A conserved Ile Leu Leu sequence within the CWB2 repeats is essential for correct anchoring, although a preceding proline residue is dispensable.
25649385	5	43	theme	conserved	845:853	arg1	essential					903:911	essential	903:911	essential	903:911	A conserved Ile Leu Leu sequence within the CWB2 repeats is essential for correct anchoring, although a preceding proline residue is dispensable.
25649385	7	44	theme	cell	1206:1209	arg1	proteins					1216:1223	two cell wall proteins	1202:1223	two cell wall proteins	1202:1223	We show that the anionic polymer PSII binds two cell wall proteins, SlpA and Cwp2, and these interactions require the CWB2 repeats, defining a new mechanism of protein anchoring in Gram-positive bacteria.
25649385	7	44	theme	cell	1206:1209	arg1	Cwp2					1235:1238	Cwp2	1235:1238	Cwp2	1235:1238	We show that the anionic polymer PSII binds two cell wall proteins, SlpA and Cwp2, and these interactions require the CWB2 repeats, defining a new mechanism of protein anchoring in Gram-positive bacteria.
25649385	7	44	theme	cell	1206:1209	arg1	SlpA					1226:1229	SlpA	1226:1229	SlpA	1226:1229	We show that the anionic polymer PSII binds two cell wall proteins, SlpA and Cwp2, and these interactions require the CWB2 repeats, defining a new mechanism of protein anchoring in Gram-positive bacteria.
25649385	3	45	dep	show	555:558	arg1	essential					607:615	essential	607:615	essential	607:615	In the Clostridium difficile cell wall protein family, we show the three tandem repeats of the CWB2 motif are essential for correct anchoring to the cell wall.
25649385	1	46	theme	cell	288:291	arg1	organisation					302:313	cell envelope organisation	288:313	cell envelope organisation	288:313	Gram-positive surface proteins can be covalently or non-covalently anchored to the cell wall and can impart important properties on the bacterium in respect of cell envelope organisation and interaction with the environment.
25649385	3	47	theme	motif	597:601	arg1	repeats					577:583	the three tandem repeats	560:583	the three tandem repeats of the CWB2 motif	560:601	In the Clostridium difficile cell wall protein family, we show the three tandem repeats of the CWB2 motif are essential for correct anchoring to the cell wall.
25649385	3	48	theme	tandem	570:575	arg1	repeats					577:583	the three tandem repeats	560:583	the three tandem repeats of the CWB2 motif	560:601	In the Clostridium difficile cell wall protein family, we show the three tandem repeats of the CWB2 motif are essential for correct anchoring to the cell wall.
25649385	7	49	theme	CWB2	1276:1279	arg1	repeats					1281:1287	the CWB2 repeats	1272:1287	the CWB2 repeats	1272:1287	We show that the anionic polymer PSII binds two cell wall proteins, SlpA and Cwp2, and these interactions require the CWB2 repeats, defining a new mechanism of protein anchoring in Gram-positive bacteria.
25649385	1	50	theme	cell	211:214	arg1	wall					216:219	the cell wall	207:219	the cell wall	207:219	Gram-positive surface proteins can be covalently or non-covalently anchored to the cell wall and can impart important properties on the bacterium in respect of cell envelope organisation and interaction with the environment.
25649385	1	51	theme	envelope	293:300	arg1	organisation					302:313	cell envelope organisation	288:313	cell envelope organisation	288:313	Gram-positive surface proteins can be covalently or non-covalently anchored to the cell wall and can impart important properties on the bacterium in respect of cell envelope organisation and interaction with the environment.
25649385	5	52	theme	correct	917:923	arg1	anchoring					925:933	correct anchoring	917:933	correct anchoring	917:933	A conserved Ile Leu Leu sequence within the CWB2 repeats is essential for correct anchoring, although a preceding proline residue is dispensable.
25649385	6	53	theme	anionic	1049:1055	arg1	PSII					1065:1068	the anionic polymer PSII	1045:1068	the anionic polymer PSII	1045:1068	We propose a likely genetic locus encoding synthesis of the anionic polymer PSII and, using RNA knock-down of key genes, reveal subtle effects on cell wall composition.
25649385	5	54	theme	Leu	859:861	arg1	sequence					867:874	A conserved Ile Leu Leu sequence	843:874	A conserved Ile Leu Leu sequence within the CWB2 repeats	843:898	A conserved Ile Leu Leu sequence within the CWB2 repeats is essential for correct anchoring, although a preceding proline residue is dispensable.
25649385	5	54	theme	Leu	859:861	arg1	essential					903:911	essential	903:911	essential	903:911	A conserved Ile Leu Leu sequence within the CWB2 repeats is essential for correct anchoring, although a preceding proline residue is dispensable.
25649385	2	55	theme	large	443:447	arg1	number					449:454	a large number	441:454	a large number of cell wall proteins in the Firmicutes	441:494	We describe here a mechanism of protein anchoring involving tandem CWB2 motifs found in a large number of cell wall proteins in the Firmicutes.
25649385	1	56	theme	organisation	302:313	arg1	respect					277:283	respect	277:283	respect of cell envelope organisation and interaction with the environment	277:350	Gram-positive surface proteins can be covalently or non-covalently anchored to the cell wall and can impart important properties on the bacterium in respect of cell envelope organisation and interaction with the environment.
25649385	6	57	theme	wall	1140:1143	arg1	composition					1145:1155	cell wall composition	1135:1155	cell wall composition	1135:1155	We propose a likely genetic locus encoding synthesis of the anionic polymer PSII and, using RNA knock-down of key genes, reveal subtle effects on cell wall composition.
25649385	5	58	theme	Leu	863:865	arg1	sequence					867:874	A conserved Ile Leu Leu sequence	843:874	A conserved Ile Leu Leu sequence within the CWB2 repeats	843:898	A conserved Ile Leu Leu sequence within the CWB2 repeats is essential for correct anchoring, although a preceding proline residue is dispensable.
25649385	5	58	theme	Leu	863:865	arg1	essential					903:911	essential	903:911	essential	903:911	A conserved Ile Leu Leu sequence within the CWB2 repeats is essential for correct anchoring, although a preceding proline residue is dispensable.
25649385	6	59	theme	subtle	1117:1122	arg1	effects					1124:1130	subtle effects	1117:1130	subtle effects on cell wall composition	1117:1155	We propose a likely genetic locus encoding synthesis of the anionic polymer PSII and, using RNA knock-down of key genes, reveal subtle effects on cell wall composition.
25649385	2	60	from	Firmicutes	485:494	arg1	number					449:454	a large number	441:454	a large number of cell wall proteins in the Firmicutes	441:494	We describe here a mechanism of protein anchoring involving tandem CWB2 motifs found in a large number of cell wall proteins in the Firmicutes.
25649385	6	61	theme	cell	1135:1138	arg1	composition					1145:1155	cell wall composition	1135:1155	cell wall composition	1135:1155	We propose a likely genetic locus encoding synthesis of the anionic polymer PSII and, using RNA knock-down of key genes, reveal subtle effects on cell wall composition.
25649385	6	62	theme	PSII	1065:1068	arg1	synthesis					1032:1040	synthesis	1032:1040	synthesis of the anionic polymer PSII	1032:1068	We propose a likely genetic locus encoding synthesis of the anionic polymer PSII and, using RNA knock-down of key genes, reveal subtle effects on cell wall composition.
25649385	6	63	theme	RNA	1081:1083	arg1	knock-down					1085:1094	RNA knock-down	1081:1094	RNA knock-down of key genes	1081:1107	We propose a likely genetic locus encoding synthesis of the anionic polymer PSII and, using RNA knock-down of key genes, reveal subtle effects on cell wall composition.
25649385	6	64	theme	key	1099:1101	arg1	genes					1103:1107	key genes	1099:1107	key genes	1099:1107	We propose a likely genetic locus encoding synthesis of the anionic polymer PSII and, using RNA knock-down of key genes, reveal subtle effects on cell wall composition.
25649385	3	65	theme	correct	621:627	arg1	anchoring					629:637	correct anchoring	621:637	correct anchoring to the cell wall	621:654	In the Clostridium difficile cell wall protein family, we show the three tandem repeats of the CWB2 motif are essential for correct anchoring to the cell wall.
25649385	3	66	theme	CWB2	592:595	arg1	motif					597:601	the CWB2 motif	588:601	the CWB2 motif	588:601	In the Clostridium difficile cell wall protein family, we show the three tandem repeats of the CWB2 motif are essential for correct anchoring to the cell wall.
25649385	6	67	theme	genes	1103:1107	arg1	knock-down					1085:1094	RNA knock-down	1081:1094	RNA knock-down of key genes	1081:1107	We propose a likely genetic locus encoding synthesis of the anionic polymer PSII and, using RNA knock-down of key genes, reveal subtle effects on cell wall composition.
25649385	6	68	theme	polymer	1057:1063	arg1	PSII					1065:1068	the anionic polymer PSII	1045:1068	the anionic polymer PSII	1045:1068	We propose a likely genetic locus encoding synthesis of the anionic polymer PSII and, using RNA knock-down of key genes, reveal subtle effects on cell wall composition.
25649385	7	69	theme	polymer	1183:1189	arg1	PSII					1191:1194	the anionic polymer PSII	1171:1194	the anionic polymer PSII	1171:1194	We show that the anionic polymer PSII binds two cell wall proteins, SlpA and Cwp2, and these interactions require the CWB2 repeats, defining a new mechanism of protein anchoring in Gram-positive bacteria.
25649385	2	70	theme	anchoring	393:401	arg1	mechanism					372:380	a mechanism	370:380	a mechanism of protein anchoring involving tandem CWB2 motifs found in a large number of cell wall proteins in the Firmicutes	370:494	We describe here a mechanism of protein anchoring involving tandem CWB2 motifs found in a large number of cell wall proteins in the Firmicutes.
25649385	6	71	dep	propose	992:998	arg1	reveal					1110:1115	reveal	1110:1115	reveal subtle effects on cell wall composition	1110:1155	We propose a likely genetic locus encoding synthesis of the anionic polymer PSII and, using RNA knock-down of key genes, reveal subtle effects on cell wall composition.
25649385	1	72	theme	Gram-positive	128:140	arg1	proteins					150:157	Gram-positive surface proteins	128:157	Gram-positive surface proteins	128:157	Gram-positive surface proteins can be covalently or non-covalently anchored to the cell wall and can impart important properties on the bacterium in respect of cell envelope organisation and interaction with the environment.
25649385	4	73	contain	containing	793:802	arg1	proteins					784:791	proteins	784:791	proteins containing variations in the patterns of repeats	784:840	CWB2 repeats are non-identical and cannot substitute for each other, as shown by the secretion into the culture supernatant of proteins containing variations in the patterns of repeats.
25649385	4	73	contain	containing	793:802	arg2	variations					804:813	variations	804:813	variations in the patterns of repeats	804:840	CWB2 repeats are non-identical and cannot substitute for each other, as shown by the secretion into the culture supernatant of proteins containing variations in the patterns of repeats.
25649385	0	74	theme	cell	59:62	arg1	wall					64:67	the cell wall	55:67	the cell wall using CWB2 motifs that recognise the anionic polymer PSII	55:125	Clostridium difficile surface proteins are anchored to the cell wall using CWB2 motifs that recognise the anionic polymer PSII.
25649385	1	75	theme	interaction	319:329	arg1	respect					277:283	respect	277:283	respect of cell envelope organisation and interaction with the environment	277:350	Gram-positive surface proteins can be covalently or non-covalently anchored to the cell wall and can impart important properties on the bacterium in respect of cell envelope organisation and interaction with the environment.
25649385	5	76	theme	preceding	947:955	arg1	residue					965:971	a preceding proline residue	945:971	a preceding proline residue	945:971	A conserved Ile Leu Leu sequence within the CWB2 repeats is essential for correct anchoring, although a preceding proline residue is dispensable.
25649385	5	76	theme	preceding	947:955	arg1	dispensable					976:986	dispensable	976:986	dispensable	976:986	A conserved Ile Leu Leu sequence within the CWB2 repeats is essential for correct anchoring, although a preceding proline residue is dispensable.
25649385	1	77	theme	surface	142:148	arg1	proteins					150:157	Gram-positive surface proteins	128:157	Gram-positive surface proteins	128:157	Gram-positive surface proteins can be covalently or non-covalently anchored to the cell wall and can impart important properties on the bacterium in respect of cell envelope organisation and interaction with the environment.
25649385	6	78	from	effects	1124:1130	arg1	composition					1145:1155	cell wall composition	1135:1155	cell wall composition	1135:1155	We propose a likely genetic locus encoding synthesis of the anionic polymer PSII and, using RNA knock-down of key genes, reveal subtle effects on cell wall composition.
26444829	0	0	theme	Surface	97:103	arg1	Coatings					105:112	Different Surface Coatings	87:112	Different Surface Coatings	87:112	Proteomics Analysis Reveals Distinct Corona Composition on Magnetic Nanoparticles with Different Surface Coatings: Implications for Interactions with Primary Human Macrophages.
26444829	2	1	theme	biological	505:514	arg1	behavior					516:523	their biological behavior	499:523	their biological behavior	499:523	The influence of different surface coatings on the biocompatibility of SPIONs has been addressed, but the potential impact of the so-called corona of adsorbed proteins on the surface of SPIONs on their biological behavior is less well studied.
26444829	1	2	theme	Superparamagnetic	177:193	arg1	SPIONs					221:226	SPIONs	221:226	SPIONs	221:226	Superparamagnetic iron oxide nanoparticles (SPIONs) have emerged as promising contrast agents for magnetic resonance imaging.
26444829	1	2	theme	Superparamagnetic	177:193	arg1	nanoparticles					206:218	Superparamagnetic iron oxide nanoparticles	177:218	Superparamagnetic iron oxide nanoparticles (SPIONs)	177:227	Superparamagnetic iron oxide nanoparticles (SPIONs) have emerged as promising contrast agents for magnetic resonance imaging.
26444829	1	3	theme	magnetic	275:282	arg1	imaging					294:300	magnetic resonance imaging	275:300	magnetic resonance imaging	275:300	Superparamagnetic iron oxide nanoparticles (SPIONs) have emerged as promising contrast agents for magnetic resonance imaging.
26444829	0	4	theme	Different	87:95	arg1	Coatings					105:112	Different Surface Coatings	87:112	Different Surface Coatings	87:112	Proteomics Analysis Reveals Distinct Corona Composition on Magnetic Nanoparticles with Different Surface Coatings: Implications for Interactions with Primary Human Macrophages.
26444829	1	5	theme	iron	195:198	arg1	SPIONs					221:226	SPIONs	221:226	SPIONs	221:226	Superparamagnetic iron oxide nanoparticles (SPIONs) have emerged as promising contrast agents for magnetic resonance imaging.
26444829	1	5	theme	iron	195:198	arg1	nanoparticles					206:218	Superparamagnetic iron oxide nanoparticles	177:218	Superparamagnetic iron oxide nanoparticles (SPIONs)	177:227	Superparamagnetic iron oxide nanoparticles (SPIONs) have emerged as promising contrast agents for magnetic resonance imaging.
26444829	1	6	theme	resonance	284:292	arg1	imaging					294:300	magnetic resonance imaging	275:300	magnetic resonance imaging	275:300	Superparamagnetic iron oxide nanoparticles (SPIONs) have emerged as promising contrast agents for magnetic resonance imaging.
26444829	6	7	theme	SPIONs	1271:1276	arg1	internalization					1237:1251	internalization	1237:1251	internalization of dextran-coated SPIONs	1237:1276	Moreover, the viability of primary human monocyte-derived macrophages was influenced by the protein corona on silica-coated, but not dextran-coated SPIONs, and the protein corona promoted cellular uptake of silica-coated SPIONs, but did not affect internalization of dextran-coated SPIONs.
26444829	2	8	theme	different	320:328	arg1	coatings					338:345	different surface coatings	320:345	different surface coatings	320:345	The influence of different surface coatings on the biocompatibility of SPIONs has been addressed, but the potential impact of the so-called corona of adsorbed proteins on the surface of SPIONs on their biological behavior is less well studied.
26444829	2	9	theme	surface	330:336	arg1	coatings					338:345	different surface coatings	320:345	different surface coatings	320:345	The influence of different surface coatings on the biocompatibility of SPIONs has been addressed, but the potential impact of the so-called corona of adsorbed proteins on the surface of SPIONs on their biological behavior is less well studied.
26444829	6	10	theme	primary	1016:1022	arg1	macrophages					1047:1057	primary human monocyte-derived macrophages	1016:1057	primary human monocyte-derived macrophages	1016:1057	Moreover, the viability of primary human monocyte-derived macrophages was influenced by the protein corona on silica-coated, but not dextran-coated SPIONs, and the protein corona promoted cellular uptake of silica-coated SPIONs, but did not affect internalization of dextran-coated SPIONs.
26444829	6	11	from	corona	1089:1094	arg1	SPIONs					1137:1142	silica-coated, but not dextran-coated SPIONs	1099:1142	silica-coated, but not dextran-coated SPIONs	1099:1142	Moreover, the viability of primary human monocyte-derived macrophages was influenced by the protein corona on silica-coated, but not dextran-coated SPIONs, and the protein corona promoted cellular uptake of silica-coated SPIONs, but did not affect internalization of dextran-coated SPIONs.
26444829	6	12	link	monocyte-derived	1030:1045	arg1	macrophages					1047:1057	primary human monocyte-derived macrophages	1016:1057	primary human monocyte-derived macrophages	1016:1057	Moreover, the viability of primary human monocyte-derived macrophages was influenced by the protein corona on silica-coated, but not dextran-coated SPIONs, and the protein corona promoted cellular uptake of silica-coated SPIONs, but did not affect internalization of dextran-coated SPIONs.
26444829	0	13	with	Nanoparticles	68:80	arg1	Coatings					105:112	Different Surface Coatings	87:112	Different Surface Coatings	87:112	Proteomics Analysis Reveals Distinct Corona Composition on Magnetic Nanoparticles with Different Surface Coatings: Implications for Interactions with Primary Human Macrophages.
26444829	4	14	dep	ontology	725:732	arg1	GO					735:736	GO	735:736	GO	735:736	Notably, gene ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed distinct protein corona compositions for the two different SPIONs.
26444829	1	15	theme	oxide	200:204	arg1	SPIONs					221:226	SPIONs	221:226	SPIONs	221:226	Superparamagnetic iron oxide nanoparticles (SPIONs) have emerged as promising contrast agents for magnetic resonance imaging.
26444829	1	15	theme	oxide	200:204	arg1	nanoparticles					206:218	Superparamagnetic iron oxide nanoparticles	177:218	Superparamagnetic iron oxide nanoparticles (SPIONs)	177:227	Superparamagnetic iron oxide nanoparticles (SPIONs) have emerged as promising contrast agents for magnetic resonance imaging.
26444829	6	16	theme	monocyte-derived	1030:1045	arg1	macrophages					1047:1057	primary human monocyte-derived macrophages	1016:1057	primary human monocyte-derived macrophages	1016:1057	Moreover, the viability of primary human monocyte-derived macrophages was influenced by the protein corona on silica-coated, but not dextran-coated SPIONs, and the protein corona promoted cellular uptake of silica-coated SPIONs, but did not affect internalization of dextran-coated SPIONs.
26444829	6	17	theme	SPIONs	1210:1215	arg1	uptake					1186:1191	cellular uptake	1177:1191	cellular uptake of silica-coated SPIONs	1177:1215	Moreover, the viability of primary human monocyte-derived macrophages was influenced by the protein corona on silica-coated, but not dextran-coated SPIONs, and the protein corona promoted cellular uptake of silica-coated SPIONs, but did not affect internalization of dextran-coated SPIONs.
26444829	6	18	theme	dextran-coated	1122:1135	arg1	SPIONs					1137:1142	silica-coated, but not dextran-coated SPIONs	1099:1142	silica-coated, but not dextran-coated SPIONs	1099:1142	Moreover, the viability of primary human monocyte-derived macrophages was influenced by the protein corona on silica-coated, but not dextran-coated SPIONs, and the protein corona promoted cellular uptake of silica-coated SPIONs, but did not affect internalization of dextran-coated SPIONs.
26444829	2	19	theme	SPIONs	489:494	arg1	surface					478:484	the surface	474:484	the surface of SPIONs	474:494	The influence of different surface coatings on the biocompatibility of SPIONs has been addressed, but the potential impact of the so-called corona of adsorbed proteins on the surface of SPIONs on their biological behavior is less well studied.
26444829	4	20	theme	different	885:893	arg1	SPIONs					895:900	the two different SPIONs	877:900	the two different SPIONs	877:900	Notably, gene ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed distinct protein corona compositions for the two different SPIONs.
26444829	6	21	theme	silica-coated	1196:1208	arg1	SPIONs					1210:1215	silica-coated SPIONs	1196:1215	silica-coated SPIONs	1196:1215	Moreover, the viability of primary human monocyte-derived macrophages was influenced by the protein corona on silica-coated, but not dextran-coated SPIONs, and the protein corona promoted cellular uptake of silica-coated SPIONs, but did not affect internalization of dextran-coated SPIONs.
26444829	2	22	theme	potential	409:417	arg1	impact					419:424	the potential impact	405:424	the potential impact of the so-called corona of adsorbed proteins on the surface of SPIONs on their biological behavior	405:523	The influence of different surface coatings on the biocompatibility of SPIONs has been addressed, but the potential impact of the so-called corona of adsorbed proteins on the surface of SPIONs on their biological behavior is less well studied.
26444829	0	23	theme	Proteomics	0:9	arg1	Analysis					11:18	Proteomics Analysis	0:18	Proteomics Analysis	0:18	Proteomics Analysis Reveals Distinct Corona Composition on Magnetic Nanoparticles with Different Surface Coatings: Implications for Interactions with Primary Human Macrophages.
26444829	4	24	dep	analysis	750:757	arg1	analysis					818:825	pathway analysis	810:825	pathway analysis	810:825	Notably, gene ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed distinct protein corona compositions for the two different SPIONs.
26444829	6	25	theme	silica-coated	1099:1111	arg1	SPIONs					1137:1142	silica-coated, but not dextran-coated SPIONs	1099:1142	silica-coated, but not dextran-coated SPIONs	1099:1142	Moreover, the viability of primary human monocyte-derived macrophages was influenced by the protein corona on silica-coated, but not dextran-coated SPIONs, and the protein corona promoted cellular uptake of silica-coated SPIONs, but did not affect internalization of dextran-coated SPIONs.
26444829	0	26	theme	Distinct	28:35	arg1	Composition					44:54	Distinct Corona Composition	28:54	Distinct Corona Composition	28:54	Proteomics Analysis Reveals Distinct Corona Composition on Magnetic Nanoparticles with Different Surface Coatings: Implications for Interactions with Primary Human Macrophages.
26444829	4	27	theme	corona	853:858	arg1	compositions					860:871	distinct protein corona compositions	836:871	distinct protein corona compositions for the two different SPIONs	836:900	Notably, gene ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed distinct protein corona compositions for the two different SPIONs.
26444829	2	28	theme	coatings	338:345	arg1	influence					307:315	The influence	303:315	The influence of different surface coatings on the biocompatibility of SPIONs	303:379	The influence of different surface coatings on the biocompatibility of SPIONs has been addressed, but the potential impact of the so-called corona of adsorbed proteins on the surface of SPIONs on their biological behavior is less well studied.
26444829	4	29	theme	protein	845:851	arg1	compositions					860:871	distinct protein corona compositions	836:871	distinct protein corona compositions for the two different SPIONs	836:900	Notably, gene ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed distinct protein corona compositions for the two different SPIONs.
26444829	2	30	theme	adsorbed	453:460	arg1	proteins					462:469	adsorbed proteins	453:469	adsorbed proteins	453:469	The influence of different surface coatings on the biocompatibility of SPIONs has been addressed, but the potential impact of the so-called corona of adsorbed proteins on the surface of SPIONs on their biological behavior is less well studied.
26444829	5	31	theme	protein	973:979	arg1	corona					981:986	a protein corona	971:986	a protein corona	971:986	Relaxivity of silica-coated SPIONs was modulated by the presence of a protein corona.
26444829	3	32	theme	spectrometry-based	669:686	arg1	approaches					699:708	mass spectrometry-based proteomics approaches	664:708	mass spectrometry-based proteomics approaches	664:708	Here, we determined the composition of the plasma protein corona on silica-coated versus dextran-coated SPIONs using mass spectrometry-based proteomics approaches.
26444829	0	33	dep	Nanoparticles	68:80	arg1	Implications					115:126	Implications	115:126	Magnetic Nanoparticles with Different Surface Coatings: Implications for Interactions with Primary Human Macrophages	59:174	Proteomics Analysis Reveals Distinct Corona Composition on Magnetic Nanoparticles with Different Surface Coatings: Implications for Interactions with Primary Human Macrophages.
26444829	2	34	theme	corona	443:448	arg1	impact					419:424	the potential impact	405:424	the potential impact of the so-called corona of adsorbed proteins on the surface of SPIONs on their biological behavior	405:523	The influence of different surface coatings on the biocompatibility of SPIONs has been addressed, but the potential impact of the so-called corona of adsorbed proteins on the surface of SPIONs on their biological behavior is less well studied.
26444829	0	35	theme	Human	158:162	arg1	Macrophages					164:174	Primary Human Macrophages	150:174	Primary Human Macrophages	150:174	Proteomics Analysis Reveals Distinct Corona Composition on Magnetic Nanoparticles with Different Surface Coatings: Implications for Interactions with Primary Human Macrophages.
26444829	3	36	theme	proteomics	688:697	arg1	approaches					699:708	mass spectrometry-based proteomics approaches	664:708	mass spectrometry-based proteomics approaches	664:708	Here, we determined the composition of the plasma protein corona on silica-coated versus dextran-coated SPIONs using mass spectrometry-based proteomics approaches.
26444829	3	37	theme	corona	605:610	arg1	composition					571:581	the composition	567:581	the composition of the plasma protein corona on silica-coated versus dextran-coated SPIONs	567:656	Here, we determined the composition of the plasma protein corona on silica-coated versus dextran-coated SPIONs using mass spectrometry-based proteomics approaches.
26444829	2	38	theme	so-called	433:441	arg1	corona					443:448	the so-called corona	429:448	the so-called corona of adsorbed proteins	429:469	The influence of different surface coatings on the biocompatibility of SPIONs has been addressed, but the potential impact of the so-called corona of adsorbed proteins on the surface of SPIONs on their biological behavior is less well studied.
26444829	0	39	theme	Corona	37:42	arg1	Composition					44:54	Distinct Corona Composition	28:54	Distinct Corona Composition	28:54	Proteomics Analysis Reveals Distinct Corona Composition on Magnetic Nanoparticles with Different Surface Coatings: Implications for Interactions with Primary Human Macrophages.
26444829	0	40	theme	Primary	150:156	arg1	Macrophages					164:174	Primary Human Macrophages	150:174	Primary Human Macrophages	150:174	Proteomics Analysis Reveals Distinct Corona Composition on Magnetic Nanoparticles with Different Surface Coatings: Implications for Interactions with Primary Human Macrophages.
26444829	6	41	theme	human	1024:1028	arg1	macrophages					1047:1057	primary human monocyte-derived macrophages	1016:1057	primary human monocyte-derived macrophages	1016:1057	Moreover, the viability of primary human monocyte-derived macrophages was influenced by the protein corona on silica-coated, but not dextran-coated SPIONs, and the protein corona promoted cellular uptake of silica-coated SPIONs, but did not affect internalization of dextran-coated SPIONs.
26444829	2	42	theme	proteins	462:469	arg1	corona					443:448	the so-called corona	429:448	the so-called corona of adsorbed proteins	429:469	The influence of different surface coatings on the biocompatibility of SPIONs has been addressed, but the potential impact of the so-called corona of adsorbed proteins on the surface of SPIONs on their biological behavior is less well studied.
26444829	4	43	theme	Genes	785:789	arg1	analysis					750:757	gene ontology (GO) enrichment analysis	720:757	gene ontology (GO) enrichment analysis	720:757	Notably, gene ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed distinct protein corona compositions for the two different SPIONs.
26444829	0	44	theme	Magnetic	59:66	arg1	Nanoparticles					68:80	Magnetic Nanoparticles	59:80	Magnetic Nanoparticles with Different Surface Coatings: Implications for Interactions with Primary Human Macrophages	59:174	Proteomics Analysis Reveals Distinct Corona Composition on Magnetic Nanoparticles with Different Surface Coatings: Implications for Interactions with Primary Human Macrophages.
26444829	5	45	theme	silica-coated	917:929	arg1	SPIONs					931:936	silica-coated SPIONs	917:936	silica-coated SPIONs	917:936	Relaxivity of silica-coated SPIONs was modulated by the presence of a protein corona.
26444829	3	46	theme	mass	664:667	arg1	approaches					699:708	mass spectrometry-based proteomics approaches	664:708	mass spectrometry-based proteomics approaches	664:708	Here, we determined the composition of the plasma protein corona on silica-coated versus dextran-coated SPIONs using mass spectrometry-based proteomics approaches.
26444829	3	47	theme	plasma	590:595	arg1	corona					605:610	the plasma protein corona	586:610	the plasma protein corona	586:610	Here, we determined the composition of the plasma protein corona on silica-coated versus dextran-coated SPIONs using mass spectrometry-based proteomics approaches.
26444829	4	48	theme	enrichment	739:748	arg1	analysis					750:757	gene ontology (GO) enrichment analysis	720:757	gene ontology (GO) enrichment analysis	720:757	Notably, gene ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed distinct protein corona compositions for the two different SPIONs.
26444829	5	49	theme	corona	981:986	arg1	presence					959:966	the presence	955:966	the presence of a protein corona	955:986	Relaxivity of silica-coated SPIONs was modulated by the presence of a protein corona.
26444829	3	50	theme	protein	597:603	arg1	corona					605:610	the plasma protein corona	586:610	the plasma protein corona	586:610	Here, we determined the composition of the plasma protein corona on silica-coated versus dextran-coated SPIONs using mass spectrometry-based proteomics approaches.
26444829	2	51	theme	SPIONs	374:379	arg1	biocompatibility					354:369	the biocompatibility	350:369	the biocompatibility of SPIONs	350:379	The influence of different surface coatings on the biocompatibility of SPIONs has been addressed, but the potential impact of the so-called corona of adsorbed proteins on the surface of SPIONs on their biological behavior is less well studied.
26444829	2	52	from	influence	307:315	arg1	biocompatibility					354:369	the biocompatibility	350:369	the biocompatibility of SPIONs	350:379	The influence of different surface coatings on the biocompatibility of SPIONs has been addressed, but the potential impact of the so-called corona of adsorbed proteins on the surface of SPIONs on their biological behavior is less well studied.
26444829	4	53	theme	distinct	836:843	arg1	compositions					860:871	distinct protein corona compositions	836:871	distinct protein corona compositions for the two different SPIONs	836:900	Notably, gene ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed distinct protein corona compositions for the two different SPIONs.
26444829	4	54	theme	pathway	810:816	arg1	analysis					818:825	pathway analysis	810:825	pathway analysis	810:825	Notably, gene ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed distinct protein corona compositions for the two different SPIONs.
26444829	3	55	theme	dextran-coated	636:649	arg1	SPIONs					651:656	silica-coated versus dextran-coated SPIONs	615:656	silica-coated versus dextran-coated SPIONs	615:656	Here, we determined the composition of the plasma protein corona on silica-coated versus dextran-coated SPIONs using mass spectrometry-based proteomics approaches.
26444829	2	56	from	impact	419:424	arg1	behavior					516:523	their biological behavior	499:523	their biological behavior	499:523	The influence of different surface coatings on the biocompatibility of SPIONs has been addressed, but the potential impact of the so-called corona of adsorbed proteins on the surface of SPIONs on their biological behavior is less well studied.
26444829	2	56	from	impact	419:424	arg1	surface					478:484	the surface	474:484	the surface of SPIONs	474:494	The influence of different surface coatings on the biocompatibility of SPIONs has been addressed, but the potential impact of the so-called corona of adsorbed proteins on the surface of SPIONs on their biological behavior is less well studied.
26444829	6	57	theme	protein	1153:1159	arg1	corona					1161:1166	the protein corona	1149:1166	the protein corona	1149:1166	Moreover, the viability of primary human monocyte-derived macrophages was influenced by the protein corona on silica-coated, but not dextran-coated SPIONs, and the protein corona promoted cellular uptake of silica-coated SPIONs, but did not affect internalization of dextran-coated SPIONs.
26444829	3	58	from	composition	571:581	arg1	SPIONs					651:656	silica-coated versus dextran-coated SPIONs	615:656	silica-coated versus dextran-coated SPIONs	615:656	Here, we determined the composition of the plasma protein corona on silica-coated versus dextran-coated SPIONs using mass spectrometry-based proteomics approaches.
26444829	1	59	theme	promising	245:253	arg1	agents					264:269	promising contrast agents	245:269	promising contrast agents for magnetic resonance imaging	245:300	Superparamagnetic iron oxide nanoparticles (SPIONs) have emerged as promising contrast agents for magnetic resonance imaging.
26444829	4	60	theme	Genomes	795:801	arg1	analysis					750:757	gene ontology (GO) enrichment analysis	720:757	gene ontology (GO) enrichment analysis	720:757	Notably, gene ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed distinct protein corona compositions for the two different SPIONs.
26444829	5	61	theme	SPIONs	931:936	arg1	Relaxivity					903:912	Relaxivity	903:912	Relaxivity of silica-coated SPIONs	903:936	Relaxivity of silica-coated SPIONs was modulated by the presence of a protein corona.
26444829	1	62	theme	contrast	255:262	arg1	agents					264:269	promising contrast agents	245:269	promising contrast agents for magnetic resonance imaging	245:300	Superparamagnetic iron oxide nanoparticles (SPIONs) have emerged as promising contrast agents for magnetic resonance imaging.
26444829	3	63	theme	silica-coated	615:627	arg1	SPIONs					651:656	silica-coated versus dextran-coated SPIONs	615:656	silica-coated versus dextran-coated SPIONs	615:656	Here, we determined the composition of the plasma protein corona on silica-coated versus dextran-coated SPIONs using mass spectrometry-based proteomics approaches.
26444829	4	64	theme	ontology	725:732	arg1	analysis					750:757	gene ontology (GO) enrichment analysis	720:757	gene ontology (GO) enrichment analysis	720:757	Notably, gene ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed distinct protein corona compositions for the two different SPIONs.
26444829	0	65	with	Interactions	132:143	arg1	Macrophages					164:174	Primary Human Macrophages	150:174	Primary Human Macrophages	150:174	Proteomics Analysis Reveals Distinct Corona Composition on Magnetic Nanoparticles with Different Surface Coatings: Implications for Interactions with Primary Human Macrophages.
26444829	6	66	theme	cellular	1177:1184	arg1	uptake					1186:1191	cellular uptake	1177:1191	cellular uptake of silica-coated SPIONs	1177:1215	Moreover, the viability of primary human monocyte-derived macrophages was influenced by the protein corona on silica-coated, but not dextran-coated SPIONs, and the protein corona promoted cellular uptake of silica-coated SPIONs, but did not affect internalization of dextran-coated SPIONs.
26444829	6	67	theme	macrophages	1047:1057	arg1	viability					1003:1011	the viability	999:1011	the viability of primary human monocyte-derived macrophages	999:1057	Moreover, the viability of primary human monocyte-derived macrophages was influenced by the protein corona on silica-coated, but not dextran-coated SPIONs, and the protein corona promoted cellular uptake of silica-coated SPIONs, but did not affect internalization of dextran-coated SPIONs.
26444829	6	68	theme	dextran-coated	1256:1269	arg1	SPIONs					1271:1276	dextran-coated SPIONs	1256:1276	dextran-coated SPIONs	1256:1276	Moreover, the viability of primary human monocyte-derived macrophages was influenced by the protein corona on silica-coated, but not dextran-coated SPIONs, and the protein corona promoted cellular uptake of silica-coated SPIONs, but did not affect internalization of dextran-coated SPIONs.
26444829	4	69	theme	gene	720:723	arg1	ontology					725:732	gene ontology	720:732	gene ontology (GO) enrichment analysis	720:757	Notably, gene ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed distinct protein corona compositions for the two different SPIONs.
26444829	6	70	theme	protein	1081:1087	arg1	corona					1089:1094	the protein corona	1077:1094	the protein corona on silica-coated, but not dextran-coated SPIONs	1077:1142	Moreover, the viability of primary human monocyte-derived macrophages was influenced by the protein corona on silica-coated, but not dextran-coated SPIONs, and the protein corona promoted cellular uptake of silica-coated SPIONs, but did not affect internalization of dextran-coated SPIONs.
28821034	0	0	theme	related	102:108	arg1	enzymes					110:116	related enzymes	102:116	related enzymes in Nostoc	102:126	The relationship between monosaccharide composition of extracellular polysaccharide and activities of related enzymes in Nostoc flagelliforme under different culture conditions.
28821034	1	1	theme	culture	386:392	arg1	condition					394:402	light culture condition	380:402	light culture condition	380:402	The relationship between monosaccharide composition of Nostoc flagelliforme extracellular polysaccharide (EPS) and activities of EPS synthesis enzymes under various carbon sources, nitrogen sources and light culture condition was investigated.
28821034	3	2	theme	mannose	588:594	arg1	percentage					601:610	mannose mole percentage	588:610	mannose mole percentage	588:610	Under both carbon and nitrogen sources conditions, mannose mole percentage was increased with the increase of initial mole ratio of C/N and positively related to fructose-1, 6-bisphosphatase activity, and glucuronic acid and galactose mole percentages were positively correlated with UDP-glucose dehydrogenase, while arabinose and rhamnose mole percentages were negatively associated with UDP-glucose pyrophosphorylase.
28821034	3	3	theme	UDP-glucose	821:831	arg1	dehydrogenase					833:845	UDP-glucose dehydrogenase	821:845	UDP-glucose dehydrogenase	821:845	Under both carbon and nitrogen sources conditions, mannose mole percentage was increased with the increase of initial mole ratio of C/N and positively related to fructose-1, 6-bisphosphatase activity, and glucuronic acid and galactose mole percentages were positively correlated with UDP-glucose dehydrogenase, while arabinose and rhamnose mole percentages were negatively associated with UDP-glucose pyrophosphorylase.
28821034	3	4	theme	mole	772:775	arg1	percentages					777:787	mole percentages	772:787	mole percentages	772:787	Under both carbon and nitrogen sources conditions, mannose mole percentage was increased with the increase of initial mole ratio of C/N and positively related to fructose-1, 6-bisphosphatase activity, and glucuronic acid and galactose mole percentages were positively correlated with UDP-glucose dehydrogenase, while arabinose and rhamnose mole percentages were negatively associated with UDP-glucose pyrophosphorylase.
28821034	4	5	theme	enzymes	1018:1024	arg1	activity					1026:1033	enzymes activity	1018:1033	enzymes activity	1018:1033	Different correlation between monosaccharide composition and enzymes activity from carbon and nitrogen sources conditions was found under light condition.
28821034	3	6	theme	mole	596:599	arg1	percentage					601:610	mannose mole percentage	588:610	mannose mole percentage	588:610	Under both carbon and nitrogen sources conditions, mannose mole percentage was increased with the increase of initial mole ratio of C/N and positively related to fructose-1, 6-bisphosphatase activity, and glucuronic acid and galactose mole percentages were positively correlated with UDP-glucose dehydrogenase, while arabinose and rhamnose mole percentages were negatively associated with UDP-glucose pyrophosphorylase.
28821034	1	7	theme	EPS	307:309	arg1	enzymes					321:327	EPS synthesis enzymes	307:327	EPS synthesis enzymes under various carbon sources	307:356	The relationship between monosaccharide composition of Nostoc flagelliforme extracellular polysaccharide (EPS) and activities of EPS synthesis enzymes under various carbon sources, nitrogen sources and light culture condition was investigated.
28821034	1	8	theme	synthesis	311:319	arg1	enzymes					321:327	EPS synthesis enzymes	307:327	EPS synthesis enzymes under various carbon sources	307:356	The relationship between monosaccharide composition of Nostoc flagelliforme extracellular polysaccharide (EPS) and activities of EPS synthesis enzymes under various carbon sources, nitrogen sources and light culture condition was investigated.
28821034	0	9	theme	enzymes	110:116	arg1	activities					88:97	activities	88:97	activities of related enzymes in Nostoc	88:126	The relationship between monosaccharide composition of extracellular polysaccharide and activities of related enzymes in Nostoc flagelliforme under different culture conditions.
28821034	0	9	theme	enzymes	110:116	arg1	polysaccharide					69:82	extracellular polysaccharide	55:82	extracellular polysaccharide	55:82	The relationship between monosaccharide composition of extracellular polysaccharide and activities of related enzymes in Nostoc flagelliforme under different culture conditions.
28821034	0	10	from	polysaccharide	69:82	arg1	Nostoc					121:126	Nostoc	121:126	Nostoc	121:126	The relationship between monosaccharide composition of extracellular polysaccharide and activities of related enzymes in Nostoc flagelliforme under different culture conditions.
28821034	1	11	theme	enzymes	321:327	arg1	EPS					284:286	EPS	284:286	EPS	284:286	The relationship between monosaccharide composition of Nostoc flagelliforme extracellular polysaccharide (EPS) and activities of EPS synthesis enzymes under various carbon sources, nitrogen sources and light culture condition was investigated.
28821034	1	11	theme	enzymes	321:327	arg1	activities					293:302	activities	293:302	activities of EPS synthesis enzymes under various carbon sources	293:356	The relationship between monosaccharide composition of Nostoc flagelliforme extracellular polysaccharide (EPS) and activities of EPS synthesis enzymes under various carbon sources, nitrogen sources and light culture condition was investigated.
28821034	1	11	theme	enzymes	321:327	arg1	polysaccharide					268:281	Nostoc flagelliforme extracellular polysaccharide	233:281	Nostoc flagelliforme extracellular polysaccharide (EPS)	233:287	The relationship between monosaccharide composition of Nostoc flagelliforme extracellular polysaccharide (EPS) and activities of EPS synthesis enzymes under various carbon sources, nitrogen sources and light culture condition was investigated.
28821034	4	12	theme	monosaccharide	987:1000	arg1	composition					1002:1012	monosaccharide composition	987:1012	monosaccharide composition	987:1012	Different correlation between monosaccharide composition and enzymes activity from carbon and nitrogen sources conditions was found under light condition.
28821034	3	13	dep	acid	753:756	arg1	percentages					777:787	mole percentages	772:787	mole percentages	772:787	Under both carbon and nitrogen sources conditions, mannose mole percentage was increased with the increase of initial mole ratio of C/N and positively related to fructose-1, 6-bisphosphatase activity, and glucuronic acid and galactose mole percentages were positively correlated with UDP-glucose dehydrogenase, while arabinose and rhamnose mole percentages were negatively associated with UDP-glucose pyrophosphorylase.
28821034	5	14	with	EPS	1223:1225	arg1	composition					1255:1265	desired monosaccharide composition	1232:1265	desired monosaccharide composition	1232:1265	These findings will be helpful to establish a novel fermentation process aimed to produce the N. flagelliforme EPS with desired monosaccharide composition.
28821034	1	15	theme	monosaccharide	203:216	arg1	composition					218:228	monosaccharide composition	203:228	monosaccharide composition of Nostoc flagelliforme extracellular polysaccharide (EPS) and activities of EPS synthesis enzymes under various carbon sources	203:356	The relationship between monosaccharide composition of Nostoc flagelliforme extracellular polysaccharide (EPS) and activities of EPS synthesis enzymes under various carbon sources, nitrogen sources and light culture condition was investigated.
28821034	3	16	theme	mole	655:658	arg1	ratio					660:664	initial mole ratio	647:664	initial mole ratio of C/N	647:671	Under both carbon and nitrogen sources conditions, mannose mole percentage was increased with the increase of initial mole ratio of C/N and positively related to fructose-1, 6-bisphosphatase activity, and glucuronic acid and galactose mole percentages were positively correlated with UDP-glucose dehydrogenase, while arabinose and rhamnose mole percentages were negatively associated with UDP-glucose pyrophosphorylase.
28821034	2	17	theme	significant	448:458	arg1	influences					460:469	significant influences	448:469	significant influences on both monosaccharide composition and related enzyme activities	448:534	Culture conditions showed significant influences on both monosaccharide composition and related enzyme activities.
28821034	3	18	theme	arabinose	854:862	arg1	percentages					882:892	arabinose and rhamnose mole percentages	854:892	arabinose and rhamnose mole percentages	854:892	Under both carbon and nitrogen sources conditions, mannose mole percentage was increased with the increase of initial mole ratio of C/N and positively related to fructose-1, 6-bisphosphatase activity, and glucuronic acid and galactose mole percentages were positively correlated with UDP-glucose dehydrogenase, while arabinose and rhamnose mole percentages were negatively associated with UDP-glucose pyrophosphorylase.
28821034	3	19	theme	ratio	660:664	arg1	increase					635:642	the increase	631:642	the increase of initial mole ratio of C/N	631:671	Under both carbon and nitrogen sources conditions, mannose mole percentage was increased with the increase of initial mole ratio of C/N and positively related to fructose-1, 6-bisphosphatase activity, and glucuronic acid and galactose mole percentages were positively correlated with UDP-glucose dehydrogenase, while arabinose and rhamnose mole percentages were negatively associated with UDP-glucose pyrophosphorylase.
28821034	1	20	theme	Nostoc	233:238	arg1	EPS					284:286	EPS	284:286	EPS	284:286	The relationship between monosaccharide composition of Nostoc flagelliforme extracellular polysaccharide (EPS) and activities of EPS synthesis enzymes under various carbon sources, nitrogen sources and light culture condition was investigated.
28821034	1	20	theme	Nostoc	233:238	arg1	polysaccharide					268:281	Nostoc flagelliforme extracellular polysaccharide	233:281	Nostoc flagelliforme extracellular polysaccharide (EPS)	233:287	The relationship between monosaccharide composition of Nostoc flagelliforme extracellular polysaccharide (EPS) and activities of EPS synthesis enzymes under various carbon sources, nitrogen sources and light culture condition was investigated.
28821034	0	21	theme	monosaccharide	25:38	arg1	composition					40:50	monosaccharide composition	25:50	monosaccharide composition of extracellular polysaccharide and activities of related enzymes in Nostoc	25:126	The relationship between monosaccharide composition of extracellular polysaccharide and activities of related enzymes in Nostoc flagelliforme under different culture conditions.
28821034	1	22	theme	various	335:341	arg1	sources					350:356	various carbon sources	335:356	various carbon sources	335:356	The relationship between monosaccharide composition of Nostoc flagelliforme extracellular polysaccharide (EPS) and activities of EPS synthesis enzymes under various carbon sources, nitrogen sources and light culture condition was investigated.
28821034	0	23	theme	different	148:156	arg1	conditions					166:175	different culture conditions	148:175	different culture conditions	148:175	The relationship between monosaccharide composition of extracellular polysaccharide and activities of related enzymes in Nostoc flagelliforme under different culture conditions.
28821034	4	24	theme	Different	957:965	arg1	correlation					967:977	Different correlation	957:977	Different correlation between monosaccharide composition and enzymes activity from carbon and nitrogen sources conditions	957:1077	Different correlation between monosaccharide composition and enzymes activity from carbon and nitrogen sources conditions was found under light condition.
28821034	1	25	theme	flagelliforme	240:252	arg1	EPS					284:286	EPS	284:286	EPS	284:286	The relationship between monosaccharide composition of Nostoc flagelliforme extracellular polysaccharide (EPS) and activities of EPS synthesis enzymes under various carbon sources, nitrogen sources and light culture condition was investigated.
28821034	1	25	theme	flagelliforme	240:252	arg1	polysaccharide					268:281	Nostoc flagelliforme extracellular polysaccharide	233:281	Nostoc flagelliforme extracellular polysaccharide (EPS)	233:287	The relationship between monosaccharide composition of Nostoc flagelliforme extracellular polysaccharide (EPS) and activities of EPS synthesis enzymes under various carbon sources, nitrogen sources and light culture condition was investigated.
28821034	2	26	theme	related	510:516	arg1	activities					525:534	related enzyme activities	510:534	related enzyme activities	510:534	Culture conditions showed significant influences on both monosaccharide composition and related enzyme activities.
28821034	1	27	theme	carbon	343:348	arg1	sources					350:356	various carbon sources	335:356	various carbon sources	335:356	The relationship between monosaccharide composition of Nostoc flagelliforme extracellular polysaccharide (EPS) and activities of EPS synthesis enzymes under various carbon sources, nitrogen sources and light culture condition was investigated.
28821034	3	28	theme	initial	647:653	arg1	ratio					660:664	initial mole ratio	647:664	initial mole ratio of C/N	647:671	Under both carbon and nitrogen sources conditions, mannose mole percentage was increased with the increase of initial mole ratio of C/N and positively related to fructose-1, 6-bisphosphatase activity, and glucuronic acid and galactose mole percentages were positively correlated with UDP-glucose dehydrogenase, while arabinose and rhamnose mole percentages were negatively associated with UDP-glucose pyrophosphorylase.
28821034	1	29	theme	extracellular	254:266	arg1	EPS					284:286	EPS	284:286	EPS	284:286	The relationship between monosaccharide composition of Nostoc flagelliforme extracellular polysaccharide (EPS) and activities of EPS synthesis enzymes under various carbon sources, nitrogen sources and light culture condition was investigated.
28821034	1	29	theme	extracellular	254:266	arg1	polysaccharide					268:281	Nostoc flagelliforme extracellular polysaccharide	233:281	Nostoc flagelliforme extracellular polysaccharide (EPS)	233:287	The relationship between monosaccharide composition of Nostoc flagelliforme extracellular polysaccharide (EPS) and activities of EPS synthesis enzymes under various carbon sources, nitrogen sources and light culture condition was investigated.
28821034	4	30	dep	carbon	1040:1045	arg1	conditions					1068:1077	conditions	1068:1077	conditions	1068:1077	Different correlation between monosaccharide composition and enzymes activity from carbon and nitrogen sources conditions was found under light condition.
28821034	4	31	theme	light	1095:1099	arg1	condition					1101:1109	light condition	1095:1109	light condition	1095:1109	Different correlation between monosaccharide composition and enzymes activity from carbon and nitrogen sources conditions was found under light condition.
28821034	2	32	theme	monosaccharide	479:492	arg1	composition					494:504	monosaccharide composition	479:504	monosaccharide composition	479:504	Culture conditions showed significant influences on both monosaccharide composition and related enzyme activities.
28821034	1	33	theme	polysaccharide	268:281	arg1	condition					394:402	light culture condition	380:402	light culture condition	380:402	The relationship between monosaccharide composition of Nostoc flagelliforme extracellular polysaccharide (EPS) and activities of EPS synthesis enzymes under various carbon sources, nitrogen sources and light culture condition was investigated.
28821034	1	33	theme	polysaccharide	268:281	arg1	sources					368:374	nitrogen sources	359:374	nitrogen sources	359:374	The relationship between monosaccharide composition of Nostoc flagelliforme extracellular polysaccharide (EPS) and activities of EPS synthesis enzymes under various carbon sources, nitrogen sources and light culture condition was investigated.
28821034	1	33	theme	polysaccharide	268:281	arg1	composition					218:228	monosaccharide composition	203:228	monosaccharide composition of Nostoc flagelliforme extracellular polysaccharide (EPS) and activities of EPS synthesis enzymes under various carbon sources	203:356	The relationship between monosaccharide composition of Nostoc flagelliforme extracellular polysaccharide (EPS) and activities of EPS synthesis enzymes under various carbon sources, nitrogen sources and light culture condition was investigated.
28821034	0	34	theme	culture	158:164	arg1	conditions					166:175	different culture conditions	148:175	different culture conditions	148:175	The relationship between monosaccharide composition of extracellular polysaccharide and activities of related enzymes in Nostoc flagelliforme under different culture conditions.
28821034	0	35	from	activities	88:97	arg1	Nostoc					121:126	Nostoc	121:126	Nostoc	121:126	The relationship between monosaccharide composition of extracellular polysaccharide and activities of related enzymes in Nostoc flagelliforme under different culture conditions.
28821034	2	36	theme	enzyme	518:523	arg1	activities					525:534	related enzyme activities	510:534	related enzyme activities	510:534	Culture conditions showed significant influences on both monosaccharide composition and related enzyme activities.
28821034	0	37	theme	polysaccharide	69:82	arg1	composition					40:50	monosaccharide composition	25:50	monosaccharide composition of extracellular polysaccharide and activities of related enzymes in Nostoc	25:126	The relationship between monosaccharide composition of extracellular polysaccharide and activities of related enzymes in Nostoc flagelliforme under different culture conditions.
28821034	1	38	theme	nitrogen	359:366	arg1	sources					368:374	nitrogen sources	359:374	nitrogen sources	359:374	The relationship between monosaccharide composition of Nostoc flagelliforme extracellular polysaccharide (EPS) and activities of EPS synthesis enzymes under various carbon sources, nitrogen sources and light culture condition was investigated.
28821034	4	39	theme	nitrogen	1051:1058	arg1	sources					1060:1066	nitrogen sources	1051:1066	nitrogen sources	1051:1066	Different correlation between monosaccharide composition and enzymes activity from carbon and nitrogen sources conditions was found under light condition.
28821034	4	40	from	sources	1060:1066	arg1	composition					1002:1012	monosaccharide composition	987:1012	monosaccharide composition	987:1012	Different correlation between monosaccharide composition and enzymes activity from carbon and nitrogen sources conditions was found under light condition.
28821034	4	40	from	sources	1060:1066	arg1	activity					1026:1033	enzymes activity	1018:1033	enzymes activity	1018:1033	Different correlation between monosaccharide composition and enzymes activity from carbon and nitrogen sources conditions was found under light condition.
28821034	3	41	dep	fructose-1	699:708	arg1	6-bisphosphatase					711:726	6-bisphosphatase	711:726	6-bisphosphatase	711:726	Under both carbon and nitrogen sources conditions, mannose mole percentage was increased with the increase of initial mole ratio of C/N and positively related to fructose-1, 6-bisphosphatase activity, and glucuronic acid and galactose mole percentages were positively correlated with UDP-glucose dehydrogenase, while arabinose and rhamnose mole percentages were negatively associated with UDP-glucose pyrophosphorylase.
28821034	0	42	theme	extracellular	55:67	arg1	polysaccharide					69:82	extracellular polysaccharide	55:82	extracellular polysaccharide	55:82	The relationship between monosaccharide composition of extracellular polysaccharide and activities of related enzymes in Nostoc flagelliforme under different culture conditions.
28821034	3	43	theme	rhamnose	868:875	arg1	percentages					882:892	arabinose and rhamnose mole percentages	854:892	arabinose and rhamnose mole percentages	854:892	Under both carbon and nitrogen sources conditions, mannose mole percentage was increased with the increase of initial mole ratio of C/N and positively related to fructose-1, 6-bisphosphatase activity, and glucuronic acid and galactose mole percentages were positively correlated with UDP-glucose dehydrogenase, while arabinose and rhamnose mole percentages were negatively associated with UDP-glucose pyrophosphorylase.
28821034	4	44	from	carbon	1040:1045	arg1	composition					1002:1012	monosaccharide composition	987:1012	monosaccharide composition	987:1012	Different correlation between monosaccharide composition and enzymes activity from carbon and nitrogen sources conditions was found under light condition.
28821034	4	44	from	carbon	1040:1045	arg1	activity					1026:1033	enzymes activity	1018:1033	enzymes activity	1018:1033	Different correlation between monosaccharide composition and enzymes activity from carbon and nitrogen sources conditions was found under light condition.
28821034	3	45	theme	C/N	669:671	arg1	ratio					660:664	initial mole ratio	647:664	initial mole ratio of C/N	647:671	Under both carbon and nitrogen sources conditions, mannose mole percentage was increased with the increase of initial mole ratio of C/N and positively related to fructose-1, 6-bisphosphatase activity, and glucuronic acid and galactose mole percentages were positively correlated with UDP-glucose dehydrogenase, while arabinose and rhamnose mole percentages were negatively associated with UDP-glucose pyrophosphorylase.
28821034	5	46	theme	monosaccharide	1240:1253	arg1	composition					1255:1265	desired monosaccharide composition	1232:1265	desired monosaccharide composition	1232:1265	These findings will be helpful to establish a novel fermentation process aimed to produce the N. flagelliforme EPS with desired monosaccharide composition.
28821034	3	47	theme	mole	877:880	arg1	percentages					882:892	arabinose and rhamnose mole percentages	854:892	arabinose and rhamnose mole percentages	854:892	Under both carbon and nitrogen sources conditions, mannose mole percentage was increased with the increase of initial mole ratio of C/N and positively related to fructose-1, 6-bisphosphatase activity, and glucuronic acid and galactose mole percentages were positively correlated with UDP-glucose dehydrogenase, while arabinose and rhamnose mole percentages were negatively associated with UDP-glucose pyrophosphorylase.
28821034	0	48	from	Nostoc	121:126	arg1	activities					88:97	activities	88:97	activities of related enzymes in Nostoc	88:126	The relationship between monosaccharide composition of extracellular polysaccharide and activities of related enzymes in Nostoc flagelliforme under different culture conditions.
28821034	0	48	from	Nostoc	121:126	arg1	polysaccharide					69:82	extracellular polysaccharide	55:82	extracellular polysaccharide	55:82	The relationship between monosaccharide composition of extracellular polysaccharide and activities of related enzymes in Nostoc flagelliforme under different culture conditions.
28821034	3	49	theme	fructose-1	699:708	arg1	activity					728:735	fructose-1, 6-bisphosphatase activity	699:735	fructose-1, 6-bisphosphatase activity	699:735	Under both carbon and nitrogen sources conditions, mannose mole percentage was increased with the increase of initial mole ratio of C/N and positively related to fructose-1, 6-bisphosphatase activity, and glucuronic acid and galactose mole percentages were positively correlated with UDP-glucose dehydrogenase, while arabinose and rhamnose mole percentages were negatively associated with UDP-glucose pyrophosphorylase.
28821034	2	50	from	influences	460:469	arg1	activities					525:534	related enzyme activities	510:534	related enzyme activities	510:534	Culture conditions showed significant influences on both monosaccharide composition and related enzyme activities.
28821034	2	50	from	influences	460:469	arg1	composition					494:504	monosaccharide composition	479:504	monosaccharide composition	479:504	Culture conditions showed significant influences on both monosaccharide composition and related enzyme activities.
28821034	3	51	theme	nitrogen	559:566	arg1	sources					568:574	both carbon and nitrogen sources	543:574	sources	568:574	Under both carbon and nitrogen sources conditions, mannose mole percentage was increased with the increase of initial mole ratio of C/N and positively related to fructose-1, 6-bisphosphatase activity, and glucuronic acid and galactose mole percentages were positively correlated with UDP-glucose dehydrogenase, while arabinose and rhamnose mole percentages were negatively associated with UDP-glucose pyrophosphorylase.
28821034	5	52	theme	N.	1206:1207	arg1	EPS					1223:1225	the N. flagelliforme EPS	1202:1225	the N. flagelliforme EPS with desired monosaccharide composition	1202:1265	These findings will be helpful to establish a novel fermentation process aimed to produce the N. flagelliforme EPS with desired monosaccharide composition.
28821034	3	53	theme	glucuronic	742:751	arg1	acid					753:756	glucuronic acid	742:756	glucuronic acid	742:756	Under both carbon and nitrogen sources conditions, mannose mole percentage was increased with the increase of initial mole ratio of C/N and positively related to fructose-1, 6-bisphosphatase activity, and glucuronic acid and galactose mole percentages were positively correlated with UDP-glucose dehydrogenase, while arabinose and rhamnose mole percentages were negatively associated with UDP-glucose pyrophosphorylase.
28821034	5	54	theme	desired	1232:1238	arg1	composition					1255:1265	desired monosaccharide composition	1232:1265	desired monosaccharide composition	1232:1265	These findings will be helpful to establish a novel fermentation process aimed to produce the N. flagelliforme EPS with desired monosaccharide composition.
28821034	3	55	theme	sources	568:574	arg1	conditions					576:585	both carbon and nitrogen sources conditions	543:585	both carbon and nitrogen sources conditions	543:585	Under both carbon and nitrogen sources conditions, mannose mole percentage was increased with the increase of initial mole ratio of C/N and positively related to fructose-1, 6-bisphosphatase activity, and glucuronic acid and galactose mole percentages were positively correlated with UDP-glucose dehydrogenase, while arabinose and rhamnose mole percentages were negatively associated with UDP-glucose pyrophosphorylase.
28821034	5	56	theme	flagelliforme	1209:1221	arg1	EPS					1223:1225	the N. flagelliforme EPS	1202:1225	the N. flagelliforme EPS with desired monosaccharide composition	1202:1265	These findings will be helpful to establish a novel fermentation process aimed to produce the N. flagelliforme EPS with desired monosaccharide composition.
28821034	3	57	theme	UDP-glucose	926:936	arg1	pyrophosphorylase					938:954	UDP-glucose pyrophosphorylase	926:954	UDP-glucose pyrophosphorylase	926:954	Under both carbon and nitrogen sources conditions, mannose mole percentage was increased with the increase of initial mole ratio of C/N and positively related to fructose-1, 6-bisphosphatase activity, and glucuronic acid and galactose mole percentages were positively correlated with UDP-glucose dehydrogenase, while arabinose and rhamnose mole percentages were negatively associated with UDP-glucose pyrophosphorylase.
28821034	0	58	theme	activities	88:97	arg1	composition					40:50	monosaccharide composition	25:50	monosaccharide composition of extracellular polysaccharide and activities of related enzymes in Nostoc	25:126	The relationship between monosaccharide composition of extracellular polysaccharide and activities of related enzymes in Nostoc flagelliforme under different culture conditions.
28821034	0	59	from	enzymes	110:116	arg1	Nostoc					121:126	Nostoc	121:126	Nostoc	121:126	The relationship between monosaccharide composition of extracellular polysaccharide and activities of related enzymes in Nostoc flagelliforme under different culture conditions.
28821034	5	60	theme	novel	1158:1162	arg1	process					1177:1183	a novel fermentation process	1156:1183	a novel fermentation process aimed to produce the N. flagelliforme EPS with desired monosaccharide composition	1156:1265	These findings will be helpful to establish a novel fermentation process aimed to produce the N. flagelliforme EPS with desired monosaccharide composition.
28821034	3	61	theme	carbon	548:553	arg1	sources					568:574	both carbon and nitrogen sources	543:574	sources	568:574	Under both carbon and nitrogen sources conditions, mannose mole percentage was increased with the increase of initial mole ratio of C/N and positively related to fructose-1, 6-bisphosphatase activity, and glucuronic acid and galactose mole percentages were positively correlated with UDP-glucose dehydrogenase, while arabinose and rhamnose mole percentages were negatively associated with UDP-glucose pyrophosphorylase.
28821034	1	62	theme	light	380:384	arg1	condition					394:402	light culture condition	380:402	light culture condition	380:402	The relationship between monosaccharide composition of Nostoc flagelliforme extracellular polysaccharide (EPS) and activities of EPS synthesis enzymes under various carbon sources, nitrogen sources and light culture condition was investigated.
28821034	5	63	theme	fermentation	1164:1175	arg1	process					1177:1183	a novel fermentation process	1156:1183	a novel fermentation process aimed to produce the N. flagelliforme EPS with desired monosaccharide composition	1156:1265	These findings will be helpful to establish a novel fermentation process aimed to produce the N. flagelliforme EPS with desired monosaccharide composition.
28821034	2	64	theme	Culture	422:428	arg1	conditions					430:439	Culture conditions	422:439	Culture conditions	422:439	Culture conditions showed significant influences on both monosaccharide composition and related enzyme activities.
28821034	1	65	theme	activities	293:302	arg1	condition					394:402	light culture condition	380:402	light culture condition	380:402	The relationship between monosaccharide composition of Nostoc flagelliforme extracellular polysaccharide (EPS) and activities of EPS synthesis enzymes under various carbon sources, nitrogen sources and light culture condition was investigated.
28821034	1	65	theme	activities	293:302	arg1	sources					368:374	nitrogen sources	359:374	nitrogen sources	359:374	The relationship between monosaccharide composition of Nostoc flagelliforme extracellular polysaccharide (EPS) and activities of EPS synthesis enzymes under various carbon sources, nitrogen sources and light culture condition was investigated.
28821034	1	65	theme	activities	293:302	arg1	composition					218:228	monosaccharide composition	203:228	monosaccharide composition of Nostoc flagelliforme extracellular polysaccharide (EPS) and activities of EPS synthesis enzymes under various carbon sources	203:356	The relationship between monosaccharide composition of Nostoc flagelliforme extracellular polysaccharide (EPS) and activities of EPS synthesis enzymes under various carbon sources, nitrogen sources and light culture condition was investigated.
28481524	4	0	theme	guar	775:778	arg1	systems					795:801	guar gum-containing systems	775:801	guar gum-containing systems	775:801	Lactase was successfully encapsulated in alginate-Ca(II) beads, and the inclusion of trehalose was critical for activity preservation toward treatments, being improved in guar gum-containing systems.
28481524	2	1	theme	alginate-Ca	306:316	arg1	beads					322:326	alginate-Ca(II) beads	306:326	alginate-Ca(II) beads	306:326	Due to this, the purpose of the present research was to analyze the encapsulation of lactase in alginate-Ca(II) beads in order to maintain its enzymatic activity toward freezing, freezing/thawing, and storage.
28481524	3	2	from	effect	430:435	arg1	microstructure					519:532	the microstructure	515:532	the microstructure	515:532	Also, the effect of the addition of trehalose, and arabic and guar gums and their influence on the microstructure as well as on thermal properties and molecular mobility were studied.
28481524	3	2	from	effect	430:435	arg1	properties					556:565	thermal properties	548:565	thermal properties	548:565	Also, the effect of the addition of trehalose, and arabic and guar gums and their influence on the microstructure as well as on thermal properties and molecular mobility were studied.
28481524	3	2	from	effect	430:435	arg1	mobility					581:588	molecular mobility	571:588	molecular mobility	571:588	Also, the effect of the addition of trehalose, and arabic and guar gums and their influence on the microstructure as well as on thermal properties and molecular mobility were studied.
28481524	2	3	theme	present	242:248	arg1	research					250:257	the present research	238:257	the present research	238:257	Due to this, the purpose of the present research was to analyze the encapsulation of lactase in alginate-Ca(II) beads in order to maintain its enzymatic activity toward freezing, freezing/thawing, and storage.
28481524	4	4	theme	gum-containing	780:793	arg1	systems					795:801	guar gum-containing systems	775:801	guar gum-containing systems	775:801	Lactase was successfully encapsulated in alginate-Ca(II) beads, and the inclusion of trehalose was critical for activity preservation toward treatments, being improved in guar gum-containing systems.
28481524	5	5	theme	technological	867:879	arg1	improvement					881:891	a valuable technological improvement	856:891	a valuable technological improvement	856:891	The gums increased the Tm' values, which represents a valuable technological improvement.
28481524	2	6	theme	lactase	295:301	arg1	encapsulation					278:290	the encapsulation	274:290	the encapsulation of lactase in alginate-Ca(II) beads	274:326	Due to this, the purpose of the present research was to analyze the encapsulation of lactase in alginate-Ca(II) beads in order to maintain its enzymatic activity toward freezing, freezing/thawing, and storage.
28481524	6	7	theme	outer	995:999	arg1	diameter					1001:1008	smaller outer diameter	987:1008	smaller outer diameter	987:1008	Finally, the presence of secondary excipients affected the microstructure, showing rods with smaller outer diameter and with lower compactness than alginate-Ca(II) beads.
28481524	3	8	theme	trehalose	456:464	arg1	addition					444:451	the addition	440:451	the addition of trehalose	440:464	Also, the effect of the addition of trehalose, and arabic and guar gums and their influence on the microstructure as well as on thermal properties and molecular mobility were studied.
28481524	6	9	theme	smaller	987:993	arg1	diameter					1001:1008	smaller outer diameter	987:1008	smaller outer diameter	987:1008	Finally, the presence of secondary excipients affected the microstructure, showing rods with smaller outer diameter and with lower compactness than alginate-Ca(II) beads.
28481524	7	10	theme	bead	1071:1074	arg1	composition					1076:1086	bead composition	1071:1086	bead composition	1071:1086	Also, bead composition greatly affects the size, shape, and relaxation times.
28481524	3	11	theme	guar	482:485	arg1	gums					487:490	and arabic and guar gums	467:490	and arabic and guar gums	467:490	Also, the effect of the addition of trehalose, and arabic and guar gums and their influence on the microstructure as well as on thermal properties and molecular mobility were studied.
28481524	0	12	theme	Alginate	0:7	arg1	Beads					9:13	Alginate Beads	0:13	Alginate Beads Containing Lactase	0:32	Alginate Beads Containing Lactase: Stability and Microstructure.
28481524	1	13	theme	low	153:155	arg1	stability					157:165	low stability	153:165	low stability against thermal and mechanical treatments	153:207	β-Galactosidase (lactase) is a widely used enzyme in the food industry; however, it has low stability against thermal and mechanical treatments.
28481524	1	14	from	enzyme	108:113	arg1	industry					127:134	the food industry	118:134	the food industry	118:134	β-Galactosidase (lactase) is a widely used enzyme in the food industry; however, it has low stability against thermal and mechanical treatments.
28481524	3	15	theme	arabic	471:476	arg1	gums					487:490	and arabic and guar gums	467:490	and arabic and guar gums	467:490	Also, the effect of the addition of trehalose, and arabic and guar gums and their influence on the microstructure as well as on thermal properties and molecular mobility were studied.
28481524	2	16	from	encapsulation	278:290	arg1	beads					322:326	alginate-Ca(II) beads	306:326	alginate-Ca(II) beads	306:326	Due to this, the purpose of the present research was to analyze the encapsulation of lactase in alginate-Ca(II) beads in order to maintain its enzymatic activity toward freezing, freezing/thawing, and storage.
28481524	2	17	theme	enzymatic	353:361	arg1	activity					363:370	its enzymatic activity	349:370	its enzymatic activity toward freezing, freezing/thawing, and storage	349:417	Due to this, the purpose of the present research was to analyze the encapsulation of lactase in alginate-Ca(II) beads in order to maintain its enzymatic activity toward freezing, freezing/thawing, and storage.
28481524	3	18	theme	thermal	548:554	arg1	properties					556:565	thermal properties	548:565	thermal properties	548:565	Also, the effect of the addition of trehalose, and arabic and guar gums and their influence on the microstructure as well as on thermal properties and molecular mobility were studied.
28481524	2	19	theme	research	250:257	arg1	purpose					227:233	the purpose	223:233	the purpose of the present research	223:257	Due to this, the purpose of the present research was to analyze the encapsulation of lactase in alginate-Ca(II) beads in order to maintain its enzymatic activity toward freezing, freezing/thawing, and storage.
28481524	3	20	from	influence	502:510	arg1	microstructure					519:532	the microstructure	515:532	the microstructure	515:532	Also, the effect of the addition of trehalose, and arabic and guar gums and their influence on the microstructure as well as on thermal properties and molecular mobility were studied.
28481524	3	20	from	influence	502:510	arg1	properties					556:565	thermal properties	548:565	thermal properties	548:565	Also, the effect of the addition of trehalose, and arabic and guar gums and their influence on the microstructure as well as on thermal properties and molecular mobility were studied.
28481524	3	20	from	influence	502:510	arg1	mobility					581:588	molecular mobility	571:588	molecular mobility	571:588	Also, the effect of the addition of trehalose, and arabic and guar gums and their influence on the microstructure as well as on thermal properties and molecular mobility were studied.
28481524	6	21	theme	lower	1019:1023	arg1	compactness					1025:1035	lower compactness	1019:1035	lower compactness than alginate-Ca(II) beads	1019:1062	Finally, the presence of secondary excipients affected the microstructure, showing rods with smaller outer diameter and with lower compactness than alginate-Ca(II) beads.
28481524	6	22	theme	alginate-Ca	1042:1052	arg1	beads					1058:1062	alginate-Ca(II) beads	1042:1062	alginate-Ca(II) beads	1042:1062	Finally, the presence of secondary excipients affected the microstructure, showing rods with smaller outer diameter and with lower compactness than alginate-Ca(II) beads.
28481524	1	23	contain	has	149:151	arg1	it					146:147	it	146:147	it	146:147	β-Galactosidase (lactase) is a widely used enzyme in the food industry; however, it has low stability against thermal and mechanical treatments.
28481524	1	23	contain	has	149:151	arg2	stability					157:165	low stability	153:165	low stability against thermal and mechanical treatments	153:207	β-Galactosidase (lactase) is a widely used enzyme in the food industry; however, it has low stability against thermal and mechanical treatments.
28481524	4	24	theme	activity	716:723	arg1	preservation					725:736	activity preservation	716:736	activity preservation toward treatments	716:754	Lactase was successfully encapsulated in alginate-Ca(II) beads, and the inclusion of trehalose was critical for activity preservation toward treatments, being improved in guar gum-containing systems.
28481524	1	25	theme	thermal	175:181	arg1	treatments					198:207	thermal and mechanical treatments	175:207	thermal and mechanical treatments	175:207	β-Galactosidase (lactase) is a widely used enzyme in the food industry; however, it has low stability against thermal and mechanical treatments.
28481524	6	26	with	rods	977:980	arg1	diameter					1001:1008	smaller outer diameter	987:1008	smaller outer diameter	987:1008	Finally, the presence of secondary excipients affected the microstructure, showing rods with smaller outer diameter and with lower compactness than alginate-Ca(II) beads.
28481524	6	26	with	rods	977:980	arg1	compactness					1025:1035	lower compactness	1019:1035	lower compactness than alginate-Ca(II) beads	1019:1062	Finally, the presence of secondary excipients affected the microstructure, showing rods with smaller outer diameter and with lower compactness than alginate-Ca(II) beads.
28481524	0	27	dep	Stability	35:43	arg1	Beads					9:13	Alginate Beads	0:13	Alginate Beads Containing Lactase	0:32	Alginate Beads Containing Lactase: Stability and Microstructure.
28481524	1	28	theme	used	103:106	arg1	enzyme					108:113	a widely used enzyme	94:113	a widely used enzyme in the food industry	94:134	β-Galactosidase (lactase) is a widely used enzyme in the food industry; however, it has low stability against thermal and mechanical treatments.
28481524	1	28	theme	used	103:106	arg1	β-Galactosidase					65:79	β-Galactosidase	65:79	β-Galactosidase (lactase)	65:89	β-Galactosidase (lactase) is a widely used enzyme in the food industry; however, it has low stability against thermal and mechanical treatments.
28481524	5	29	theme	Tm	827:828	arg1	values					831:836	the Tm' values	823:836	the Tm' values	823:836	The gums increased the Tm' values, which represents a valuable technological improvement.
28481524	4	30	theme	trehalose	689:697	arg1	critical					703:710	critical	703:710	critical	703:710	Lactase was successfully encapsulated in alginate-Ca(II) beads, and the inclusion of trehalose was critical for activity preservation toward treatments, being improved in guar gum-containing systems.
28481524	4	30	theme	trehalose	689:697	arg1	inclusion					676:684	the inclusion	672:684	the inclusion of trehalose	672:697	Lactase was successfully encapsulated in alginate-Ca(II) beads, and the inclusion of trehalose was critical for activity preservation toward treatments, being improved in guar gum-containing systems.
28481524	4	31	theme	alginate-Ca	645:655	arg1	beads					661:665	alginate-Ca(II) beads	645:665	alginate-Ca(II) beads	645:665	Lactase was successfully encapsulated in alginate-Ca(II) beads, and the inclusion of trehalose was critical for activity preservation toward treatments, being improved in guar gum-containing systems.
28481524	1	32	theme	mechanical	187:196	arg1	treatments					198:207	thermal and mechanical treatments	175:207	thermal and mechanical treatments	175:207	β-Galactosidase (lactase) is a widely used enzyme in the food industry; however, it has low stability against thermal and mechanical treatments.
28481524	5	33	theme	valuable	858:865	arg1	improvement					881:891	a valuable technological improvement	856:891	a valuable technological improvement	856:891	The gums increased the Tm' values, which represents a valuable technological improvement.
28481524	6	34	theme	excipients	929:938	arg1	presence					907:914	the presence	903:914	the presence of secondary excipients	903:938	Finally, the presence of secondary excipients affected the microstructure, showing rods with smaller outer diameter and with lower compactness than alginate-Ca(II) beads.
28481524	3	35	theme	molecular	571:579	arg1	mobility					581:588	molecular mobility	571:588	molecular mobility	571:588	Also, the effect of the addition of trehalose, and arabic and guar gums and their influence on the microstructure as well as on thermal properties and molecular mobility were studied.
28481524	1	36	theme	food	122:125	arg1	industry					127:134	the food industry	118:134	the food industry	118:134	β-Galactosidase (lactase) is a widely used enzyme in the food industry; however, it has low stability against thermal and mechanical treatments.
28481524	3	37	from	gums	487:490	arg1	microstructure					519:532	the microstructure	515:532	the microstructure	515:532	Also, the effect of the addition of trehalose, and arabic and guar gums and their influence on the microstructure as well as on thermal properties and molecular mobility were studied.
28481524	3	37	from	gums	487:490	arg1	properties					556:565	thermal properties	548:565	thermal properties	548:565	Also, the effect of the addition of trehalose, and arabic and guar gums and their influence on the microstructure as well as on thermal properties and molecular mobility were studied.
28481524	3	37	from	gums	487:490	arg1	mobility					581:588	molecular mobility	571:588	molecular mobility	571:588	Also, the effect of the addition of trehalose, and arabic and guar gums and their influence on the microstructure as well as on thermal properties and molecular mobility were studied.
28481524	6	38	theme	secondary	919:927	arg1	excipients					929:938	secondary excipients	919:938	secondary excipients	919:938	Finally, the presence of secondary excipients affected the microstructure, showing rods with smaller outer diameter and with lower compactness than alginate-Ca(II) beads.
28481524	3	39	theme	addition	444:451	arg1	influence					502:510	their influence	496:510	their influence on the microstructure as well as on thermal properties and molecular mobility	496:588	Also, the effect of the addition of trehalose, and arabic and guar gums and their influence on the microstructure as well as on thermal properties and molecular mobility were studied.
28481524	3	39	theme	addition	444:451	arg1	effect					430:435	the effect	426:435	the effect of the addition of trehalose	426:464	Also, the effect of the addition of trehalose, and arabic and guar gums and their influence on the microstructure as well as on thermal properties and molecular mobility were studied.
28481524	3	39	theme	addition	444:451	arg1	gums					487:490	and arabic and guar gums	467:490	and arabic and guar gums	467:490	Also, the effect of the addition of trehalose, and arabic and guar gums and their influence on the microstructure as well as on thermal properties and molecular mobility were studied.
28481524	7	40	theme	relaxation	1125:1134	arg1	times					1136:1140	relaxation times	1125:1140	relaxation times	1125:1140	Also, bead composition greatly affects the size, shape, and relaxation times.
25691527	4	0	theme	CS	612:613	arg1	transcripts					627:637	CS utilization transcripts	612:637	CS utilization transcripts upon exposure to CS	612:657	Whereas certain Bacteroides species display a transient surge in CS utilization transcripts upon exposure to CS, other species exhibit sustained activation of these genes.
25691527	10	1	theme	Bacteroides	1434:1444	arg1	species					1446:1452	gut Bacteroides species	1430:1452	gut Bacteroides species	1430:1452	For instance, gut Bacteroides species harbor orthologous nutrient breakdown genes and may face competition from one another for these nutrients.
25691527	3	2	theme	utilization	528:538	arg1	genes					540:544	orthologous CS utilization genes	513:544	orthologous CS utilization genes	513:544	Here we reveal that related Bacteroides species that can utilize the mammalian glycan chondroitin sulfate (CS) have diverged in the manner in which they temporally regulate orthologous CS utilization genes.
25691527	13	3	theme	nutrients	2190:2198	arg1	utilization					2165:2175	utilization	2165:2175	utilization of available nutrients	2165:2198	Similarly to how differential regulation of orthologous genes enables related species to access new environments, gut bacteria may regulate the same genes in distinct fashions to reduce the overlap with coexisting species for utilization of available nutrients.
25691527	13	4	dep	the	2125:2127	arg1	overlap					2129:2135	overlap	2129:2135	overlap with coexisting species for utilization of available nutrients	2129:2198	Similarly to how differential regulation of orthologous genes enables related species to access new environments, gut bacteria may regulate the same genes in distinct fashions to reduce the overlap with coexisting species for utilization of available nutrients.
25691527	7	5	theme	sole	1134:1137	arg1	CS					1124:1125	CS	1124:1125	CS	1124:1125	However, they vary in their responses to CS availability and to the composition of the bacterial community when CS is the sole carbon source.
25691527	7	5	theme	sole	1134:1137	arg1	source					1146:1151	the sole carbon source	1130:1151	the sole carbon source	1130:1151	However, they vary in their responses to CS availability and to the composition of the bacterial community when CS is the sole carbon source.
25691527	6	6	theme	expression	944:953	arg1	behaviors					955:963	distinct expression behaviors	935:963	distinct expression behaviors	935:963	Bacteroides species exhibiting distinct expression behaviors in the presence of CS can be cocultured on CS.
25691527	10	7	theme	nutrient	1473:1480	arg1	genes					1492:1496	orthologous nutrient breakdown genes	1461:1496	orthologous nutrient breakdown genes	1461:1496	For instance, gut Bacteroides species harbor orthologous nutrient breakdown genes and may face competition from one another for these nutrients.
25691527	10	8	from	one	1528:1530	arg1	competition					1511:1521	competition	1511:1521	competition from one another for these nutrients	1511:1558	For instance, gut Bacteroides species harbor orthologous nutrient breakdown genes and may face competition from one another for these nutrients.
25691527	8	9	theme	bacterial	1286:1294	arg1	species					1296:1302	gut bacterial species	1282:1302	gut bacterial species using a given nutrient	1282:1325	Our results indicate that diversity resulting from regulation of polysaccharide utilization genes may enable the coexistence of gut bacterial species using a given nutrient.
25691527	11	10	theme	microbial	1585:1593	arg1	composition					1595:1605	the gut microbial composition	1577:1605	the gut microbial composition	1577:1605	How, then, does the gut microbial composition maintain such remarkable stability over long durations?
25691527	8	11	theme	utilization	1234:1244	arg1	genes					1246:1250	polysaccharide utilization genes	1219:1250	polysaccharide utilization genes	1219:1250	Our results indicate that diversity resulting from regulation of polysaccharide utilization genes may enable the coexistence of gut bacterial species using a given nutrient.
25691527	4	12	theme	other	660:664	arg1	species					666:672	other species	660:672	other species	660:672	Whereas certain Bacteroides species display a transient surge in CS utilization transcripts upon exposure to CS, other species exhibit sustained activation of these genes.
25691527	7	13	theme	bacterial	1099:1107	arg1	community					1109:1117	the bacterial community	1095:1117	the bacterial community	1095:1117	However, they vary in their responses to CS availability and to the composition of the bacterial community when CS is the sole carbon source.
25691527	2	14	theme	stable	320:325	arg1	coexistence					327:337	their stable coexistence	314:337	their stable coexistence	314:337	Closely related species often harbor homologous nutrient utilization genes and cocolonize the gut, raising questions regarding the strategies mediating their stable coexistence.
25691527	8	15	theme	given	1312:1316	arg1	nutrient					1318:1325	a given nutrient	1310:1325	a given nutrient	1310:1325	Our results indicate that diversity resulting from regulation of polysaccharide utilization genes may enable the coexistence of gut bacterial species using a given nutrient.
25691527	5	16	theme	dissimilar	883:892	arg1	response					894:901	a dissimilar response	881:901	a dissimilar response	881:901	Remarkably, species-specific expression dynamics are retained even when the key players governing a particular response are replaced by those from a species with a dissimilar response.
25691527	12	17	theme	chondroitin	1749:1759	arg1	CS					1770:1771	CS	1770:1771	CS	1770:1771	We establish that in the case of genes conferring the ability to utilize the nutrient chondroitin sulfate (CS), microbial species vary in how they temporally regulate these genes and exhibit subtle growth differences on the basis of CS availability and community composition.
25691527	12	17	theme	chondroitin	1749:1759	arg1	sulfate					1761:1767	the nutrient chondroitin sulfate	1736:1767	the nutrient chondroitin sulfate (CS)	1736:1772	We establish that in the case of genes conferring the ability to utilize the nutrient chondroitin sulfate (CS), microbial species vary in how they temporally regulate these genes and exhibit subtle growth differences on the basis of CS availability and community composition.
25691527	6	18	theme	CS	984:985	arg1	presence					972:979	the presence	968:979	the presence of CS	968:985	Bacteroides species exhibiting distinct expression behaviors in the presence of CS can be cocultured on CS.
25691527	11	19	theme	gut	1581:1583	arg1	composition					1595:1605	the gut microbial composition	1577:1605	the gut microbial composition	1577:1605	How, then, does the gut microbial composition maintain such remarkable stability over long durations?
25691527	13	20	theme	differential	1956:1967	arg1	regulation					1969:1978	differential regulation	1956:1978	differential regulation of orthologous genes	1956:1999	Similarly to how differential regulation of orthologous genes enables related species to access new environments, gut bacteria may regulate the same genes in distinct fashions to reduce the overlap with coexisting species for utilization of available nutrients.
25691527	0	21	theme	mammalian	56:64	arg1	glycan					66:71	a mammalian glycan	54:71	a mammalian glycan	54:71	Species-specific dynamic responses of gut bacteria to a mammalian glycan.
25691527	13	22	theme	distinct	2097:2104	arg1	fashions					2106:2113	distinct fashions	2097:2113	distinct fashions	2097:2113	Similarly to how differential regulation of orthologous genes enables related species to access new environments, gut bacteria may regulate the same genes in distinct fashions to reduce the overlap with coexisting species for utilization of available nutrients.
25691527	1	23	theme	species	153:159	arg1	hundreds					131:138	hundreds	131:138	hundreds of bacterial species	131:159	UNLABELLED The mammalian intestine provides nutrients to hundreds of bacterial species.
25691527	9	24	theme	IMPORTANCE	1328:1337	arg1	Genes					1339:1343	IMPORTANCE Genes	1328:1343	IMPORTANCE Genes mediating a specific task	1328:1369	IMPORTANCE Genes mediating a specific task are typically conserved in related microbes.
25691527	12	25	theme	genes	1696:1700	arg1	case					1688:1691	the case	1684:1691	the case of genes conferring the ability to utilize the nutrient chondroitin sulfate (CS)	1684:1772	We establish that in the case of genes conferring the ability to utilize the nutrient chondroitin sulfate (CS), microbial species vary in how they temporally regulate these genes and exhibit subtle growth differences on the basis of CS availability and community composition.
25691527	3	26	theme	Bacteroides	368:378	arg1	species					380:386	related Bacteroides species	360:386	related Bacteroides species that can utilize the mammalian glycan chondroitin sulfate (CS)	360:449	Here we reveal that related Bacteroides species that can utilize the mammalian glycan chondroitin sulfate (CS) have diverged in the manner in which they temporally regulate orthologous CS utilization genes.
25691527	9	27	theme	specific	1357:1364	arg1	task					1366:1369	a specific task	1355:1369	a specific task	1355:1369	IMPORTANCE Genes mediating a specific task are typically conserved in related microbes.
25691527	0	28	theme	bacteria	42:49	arg1	responses					25:33	Species-specific dynamic responses	0:33	Species-specific dynamic responses of gut bacteria to a mammalian glycan	0:71	Species-specific dynamic responses of gut bacteria to a mammalian glycan.
25691527	1	29	theme	UNLABELLED	74:83	arg1	intestine					99:107	The mammalian intestine	85:107	UNLABELLED The mammalian intestine	74:107	UNLABELLED The mammalian intestine provides nutrients to hundreds of bacterial species.
25691527	3	30	theme	mammalian	409:417	arg1	sulfate					438:444	the mammalian glycan chondroitin sulfate	405:444	the mammalian glycan chondroitin sulfate (CS)	405:449	Here we reveal that related Bacteroides species that can utilize the mammalian glycan chondroitin sulfate (CS) have diverged in the manner in which they temporally regulate orthologous CS utilization genes.
25691527	3	30	theme	mammalian	409:417	arg1	CS					447:448	CS	447:448	CS	447:448	Here we reveal that related Bacteroides species that can utilize the mammalian glycan chondroitin sulfate (CS) have diverged in the manner in which they temporally regulate orthologous CS utilization genes.
25691527	0	31	theme	Species-specific	0:15	arg1	responses					25:33	Species-specific dynamic responses	0:33	Species-specific dynamic responses of gut bacteria to a mammalian glycan	0:71	Species-specific dynamic responses of gut bacteria to a mammalian glycan.
25691527	12	32	theme	composition	1926:1936	arg1	basis					1887:1891	the basis	1883:1891	the basis of CS availability and community composition	1883:1936	We establish that in the case of genes conferring the ability to utilize the nutrient chondroitin sulfate (CS), microbial species vary in how they temporally regulate these genes and exhibit subtle growth differences on the basis of CS availability and community composition.
25691527	1	33	theme	mammalian	89:97	arg1	intestine					99:107	The mammalian intestine	85:107	UNLABELLED The mammalian intestine	74:107	UNLABELLED The mammalian intestine provides nutrients to hundreds of bacterial species.
25691527	4	34	theme	transient	593:601	arg1	surge					603:607	a transient surge	591:607	a transient surge in CS utilization transcripts upon exposure to CS	591:657	Whereas certain Bacteroides species display a transient surge in CS utilization transcripts upon exposure to CS, other species exhibit sustained activation of these genes.
25691527	5	35	with	species	868:874	arg1	response					894:901	a dissimilar response	881:901	a dissimilar response	881:901	Remarkably, species-specific expression dynamics are retained even when the key players governing a particular response are replaced by those from a species with a dissimilar response.
25691527	0	36	theme	gut	38:40	arg1	bacteria					42:49	gut bacteria	38:49	gut bacteria	38:49	Species-specific dynamic responses of gut bacteria to a mammalian glycan.
25691527	9	37	theme	related	1398:1404	arg1	microbes					1406:1413	related microbes	1398:1413	related microbes	1398:1413	IMPORTANCE Genes mediating a specific task are typically conserved in related microbes.
25691527	12	38	theme	CS	1896:1897	arg1	availability					1899:1910	CS availability	1896:1910	CS availability	1896:1910	We establish that in the case of genes conferring the ability to utilize the nutrient chondroitin sulfate (CS), microbial species vary in how they temporally regulate these genes and exhibit subtle growth differences on the basis of CS availability and community composition.
25691527	3	39	theme	chondroitin	426:436	arg1	sulfate					438:444	the mammalian glycan chondroitin sulfate	405:444	the mammalian glycan chondroitin sulfate (CS)	405:449	Here we reveal that related Bacteroides species that can utilize the mammalian glycan chondroitin sulfate (CS) have diverged in the manner in which they temporally regulate orthologous CS utilization genes.
25691527	3	39	theme	chondroitin	426:436	arg1	CS					447:448	CS	447:448	CS	447:448	Here we reveal that related Bacteroides species that can utilize the mammalian glycan chondroitin sulfate (CS) have diverged in the manner in which they temporally regulate orthologous CS utilization genes.
25691527	2	40	theme	related	170:176	arg1	species					178:184	Closely related species	162:184	Closely related species	162:184	Closely related species often harbor homologous nutrient utilization genes and cocolonize the gut, raising questions regarding the strategies mediating their stable coexistence.
25691527	11	41	theme	long	1647:1650	arg1	durations					1652:1660	long durations	1647:1660	long durations	1647:1660	How, then, does the gut microbial composition maintain such remarkable stability over long durations?
25691527	12	42	theme	growth	1861:1866	arg1	differences					1868:1878	subtle growth differences	1854:1878	subtle growth differences on the basis of CS availability and community composition	1854:1936	We establish that in the case of genes conferring the ability to utilize the nutrient chondroitin sulfate (CS), microbial species vary in how they temporally regulate these genes and exhibit subtle growth differences on the basis of CS availability and community composition.
25691527	12	43	theme	subtle	1854:1859	arg1	differences					1868:1878	subtle growth differences	1854:1878	subtle growth differences on the basis of CS availability and community composition	1854:1936	We establish that in the case of genes conferring the ability to utilize the nutrient chondroitin sulfate (CS), microbial species vary in how they temporally regulate these genes and exhibit subtle growth differences on the basis of CS availability and community composition.
25691527	4	44	theme	Bacteroides	563:573	arg1	species					575:581	certain Bacteroides species	555:581	certain Bacteroides species	555:581	Whereas certain Bacteroides species display a transient surge in CS utilization transcripts upon exposure to CS, other species exhibit sustained activation of these genes.
25691527	2	45	theme	utilization	219:229	arg1	genes					231:235	homologous nutrient utilization genes	199:235	homologous nutrient utilization genes	199:235	Closely related species often harbor homologous nutrient utilization genes and cocolonize the gut, raising questions regarding the strategies mediating their stable coexistence.
25691527	2	46	theme	homologous	199:208	arg1	genes					231:235	homologous nutrient utilization genes	199:235	homologous nutrient utilization genes	199:235	Closely related species often harbor homologous nutrient utilization genes and cocolonize the gut, raising questions regarding the strategies mediating their stable coexistence.
25691527	13	47	theme	new	2035:2037	arg1	environments					2039:2050	new environments	2035:2050	new environments	2035:2050	Similarly to how differential regulation of orthologous genes enables related species to access new environments, gut bacteria may regulate the same genes in distinct fashions to reduce the overlap with coexisting species for utilization of available nutrients.
25691527	7	48	theme	carbon	1139:1144	arg1	CS					1124:1125	CS	1124:1125	CS	1124:1125	However, they vary in their responses to CS availability and to the composition of the bacterial community when CS is the sole carbon source.
25691527	7	48	theme	carbon	1139:1144	arg1	source					1146:1151	the sole carbon source	1130:1151	the sole carbon source	1130:1151	However, they vary in their responses to CS availability and to the composition of the bacterial community when CS is the sole carbon source.
25691527	3	49	theme	CS	525:526	arg1	genes					540:544	orthologous CS utilization genes	513:544	orthologous CS utilization genes	513:544	Here we reveal that related Bacteroides species that can utilize the mammalian glycan chondroitin sulfate (CS) have diverged in the manner in which they temporally regulate orthologous CS utilization genes.
25691527	13	50	theme	available	2180:2188	arg1	nutrients					2190:2198	available nutrients	2180:2198	available nutrients	2180:2198	Similarly to how differential regulation of orthologous genes enables related species to access new environments, gut bacteria may regulate the same genes in distinct fashions to reduce the overlap with coexisting species for utilization of available nutrients.
25691527	11	51	theme	such	1616:1619	arg1	stability					1632:1640	such remarkable stability	1616:1640	such remarkable stability	1616:1640	How, then, does the gut microbial composition maintain such remarkable stability over long durations?
25691527	10	52	theme	gut	1430:1432	arg1	species					1446:1452	gut Bacteroides species	1430:1452	gut Bacteroides species	1430:1452	For instance, gut Bacteroides species harbor orthologous nutrient breakdown genes and may face competition from one another for these nutrients.
25691527	4	53	theme	utilization	615:625	arg1	transcripts					627:637	CS utilization transcripts	612:637	CS utilization transcripts upon exposure to CS	612:657	Whereas certain Bacteroides species display a transient surge in CS utilization transcripts upon exposure to CS, other species exhibit sustained activation of these genes.
25691527	6	54	theme	distinct	935:942	arg1	behaviors					955:963	distinct expression behaviors	935:963	distinct expression behaviors	935:963	Bacteroides species exhibiting distinct expression behaviors in the presence of CS can be cocultured on CS.
25691527	4	55	theme	sustained	682:690	arg1	activation					692:701	sustained activation	682:701	sustained activation of these genes	682:716	Whereas certain Bacteroides species display a transient surge in CS utilization transcripts upon exposure to CS, other species exhibit sustained activation of these genes.
25691527	13	56	from	genes	2088:2092	arg1	fashions					2106:2113	distinct fashions	2097:2113	distinct fashions	2097:2113	Similarly to how differential regulation of orthologous genes enables related species to access new environments, gut bacteria may regulate the same genes in distinct fashions to reduce the overlap with coexisting species for utilization of available nutrients.
25691527	7	57	theme	community	1109:1117	arg1	composition					1080:1090	the composition	1076:1090	the composition of the bacterial community	1076:1117	However, they vary in their responses to CS availability and to the composition of the bacterial community when CS is the sole carbon source.
25691527	10	58	theme	orthologous	1461:1471	arg1	genes					1492:1496	orthologous nutrient breakdown genes	1461:1496	orthologous nutrient breakdown genes	1461:1496	For instance, gut Bacteroides species harbor orthologous nutrient breakdown genes and may face competition from one another for these nutrients.
25691527	12	59	theme	microbial	1775:1783	arg1	species					1785:1791	microbial species	1775:1791	microbial species	1775:1791	We establish that in the case of genes conferring the ability to utilize the nutrient chondroitin sulfate (CS), microbial species vary in how they temporally regulate these genes and exhibit subtle growth differences on the basis of CS availability and community composition.
25691527	8	60	theme	gut	1282:1284	arg1	species					1296:1302	gut bacterial species	1282:1302	gut bacterial species using a given nutrient	1282:1325	Our results indicate that diversity resulting from regulation of polysaccharide utilization genes may enable the coexistence of gut bacterial species using a given nutrient.
25691527	13	61	theme	orthologous	1983:1993	arg1	genes					1995:1999	orthologous genes	1983:1999	orthologous genes	1983:1999	Similarly to how differential regulation of orthologous genes enables related species to access new environments, gut bacteria may regulate the same genes in distinct fashions to reduce the overlap with coexisting species for utilization of available nutrients.
25691527	5	62	theme	species-specific	731:746	arg1	dynamics					759:766	species-specific expression dynamics	731:766	species-specific expression dynamics	731:766	Remarkably, species-specific expression dynamics are retained even when the key players governing a particular response are replaced by those from a species with a dissimilar response.
25691527	13	63	theme	genes	1995:1999	arg1	regulation					1969:1978	differential regulation	1956:1978	differential regulation of orthologous genes	1956:1999	Similarly to how differential regulation of orthologous genes enables related species to access new environments, gut bacteria may regulate the same genes in distinct fashions to reduce the overlap with coexisting species for utilization of available nutrients.
25691527	8	64	theme	polysaccharide	1219:1232	arg1	genes					1246:1250	polysaccharide utilization genes	1219:1250	polysaccharide utilization genes	1219:1250	Our results indicate that diversity resulting from regulation of polysaccharide utilization genes may enable the coexistence of gut bacterial species using a given nutrient.
25691527	8	65	theme	species	1296:1302	arg1	coexistence					1267:1277	the coexistence	1263:1277	the coexistence of gut bacterial species using a given nutrient	1263:1325	Our results indicate that diversity resulting from regulation of polysaccharide utilization genes may enable the coexistence of gut bacterial species using a given nutrient.
25691527	8	66	theme	genes	1246:1250	arg1	regulation					1205:1214	regulation	1205:1214	regulation of polysaccharide utilization genes	1205:1250	Our results indicate that diversity resulting from regulation of polysaccharide utilization genes may enable the coexistence of gut bacterial species using a given nutrient.
25691527	7	67	theme	CS	1053:1054	arg1	availability					1056:1067	CS availability	1053:1067	CS availability	1053:1067	However, they vary in their responses to CS availability and to the composition of the bacterial community when CS is the sole carbon source.
25691527	12	68	from	differences	1868:1878	arg1	basis					1887:1891	the basis	1883:1891	the basis of CS availability and community composition	1883:1936	We establish that in the case of genes conferring the ability to utilize the nutrient chondroitin sulfate (CS), microbial species vary in how they temporally regulate these genes and exhibit subtle growth differences on the basis of CS availability and community composition.
25691527	12	69	theme	nutrient	1740:1747	arg1	CS					1770:1771	CS	1770:1771	CS	1770:1771	We establish that in the case of genes conferring the ability to utilize the nutrient chondroitin sulfate (CS), microbial species vary in how they temporally regulate these genes and exhibit subtle growth differences on the basis of CS availability and community composition.
25691527	12	69	theme	nutrient	1740:1747	arg1	sulfate					1761:1767	the nutrient chondroitin sulfate	1736:1767	the nutrient chondroitin sulfate (CS)	1736:1772	We establish that in the case of genes conferring the ability to utilize the nutrient chondroitin sulfate (CS), microbial species vary in how they temporally regulate these genes and exhibit subtle growth differences on the basis of CS availability and community composition.
25691527	5	70	theme	key	795:797	arg1	players					799:805	the key players	791:805	the key players governing a particular response	791:837	Remarkably, species-specific expression dynamics are retained even when the key players governing a particular response are replaced by those from a species with a dissimilar response.
25691527	13	71	theme	coexisting	2142:2151	arg1	species					2153:2159	coexisting species	2142:2159	coexisting species	2142:2159	Similarly to how differential regulation of orthologous genes enables related species to access new environments, gut bacteria may regulate the same genes in distinct fashions to reduce the overlap with coexisting species for utilization of available nutrients.
25691527	1	72	theme	bacterial	143:151	arg1	species					153:159	bacterial species	143:159	bacterial species	143:159	UNLABELLED The mammalian intestine provides nutrients to hundreds of bacterial species.
25691527	3	73	theme	related	360:366	arg1	species					380:386	related Bacteroides species	360:386	related Bacteroides species that can utilize the mammalian glycan chondroitin sulfate (CS)	360:449	Here we reveal that related Bacteroides species that can utilize the mammalian glycan chondroitin sulfate (CS) have diverged in the manner in which they temporally regulate orthologous CS utilization genes.
25691527	4	74	theme	genes	712:716	arg1	activation					692:701	sustained activation	682:701	sustained activation of these genes	682:716	Whereas certain Bacteroides species display a transient surge in CS utilization transcripts upon exposure to CS, other species exhibit sustained activation of these genes.
25691527	5	75	theme	expression	748:757	arg1	dynamics					759:766	species-specific expression dynamics	731:766	species-specific expression dynamics	731:766	Remarkably, species-specific expression dynamics are retained even when the key players governing a particular response are replaced by those from a species with a dissimilar response.
25691527	10	76	theme	breakdown	1482:1490	arg1	genes					1492:1496	orthologous nutrient breakdown genes	1461:1496	orthologous nutrient breakdown genes	1461:1496	For instance, gut Bacteroides species harbor orthologous nutrient breakdown genes and may face competition from one another for these nutrients.
25691527	13	77	theme	same	2083:2086	arg1	genes					2088:2092	the same genes	2079:2092	the same genes in distinct fashions	2079:2113	Similarly to how differential regulation of orthologous genes enables related species to access new environments, gut bacteria may regulate the same genes in distinct fashions to reduce the overlap with coexisting species for utilization of available nutrients.
25691527	12	78	theme	community	1916:1924	arg1	composition					1926:1936	community composition	1916:1936	community composition	1916:1936	We establish that in the case of genes conferring the ability to utilize the nutrient chondroitin sulfate (CS), microbial species vary in how they temporally regulate these genes and exhibit subtle growth differences on the basis of CS availability and community composition.
25691527	4	79	from	surge	603:607	arg1	transcripts					627:637	CS utilization transcripts	612:637	CS utilization transcripts upon exposure to CS	612:657	Whereas certain Bacteroides species display a transient surge in CS utilization transcripts upon exposure to CS, other species exhibit sustained activation of these genes.
25691527	13	80	theme	related	2009:2015	arg1	species					2017:2023	related species	2009:2023	related species	2009:2023	Similarly to how differential regulation of orthologous genes enables related species to access new environments, gut bacteria may regulate the same genes in distinct fashions to reduce the overlap with coexisting species for utilization of available nutrients.
25691527	3	81	theme	glycan	419:424	arg1	sulfate					438:444	the mammalian glycan chondroitin sulfate	405:444	the mammalian glycan chondroitin sulfate (CS)	405:449	Here we reveal that related Bacteroides species that can utilize the mammalian glycan chondroitin sulfate (CS) have diverged in the manner in which they temporally regulate orthologous CS utilization genes.
25691527	3	81	theme	glycan	419:424	arg1	CS					447:448	CS	447:448	CS	447:448	Here we reveal that related Bacteroides species that can utilize the mammalian glycan chondroitin sulfate (CS) have diverged in the manner in which they temporally regulate orthologous CS utilization genes.
25691527	10	82	dep	one	1528:1530	arg1	another					1532:1538	another	1532:1538	another	1532:1538	For instance, gut Bacteroides species harbor orthologous nutrient breakdown genes and may face competition from one another for these nutrients.
25691527	5	83	theme	particular	819:828	arg1	response					830:837	a particular response	817:837	a particular response	817:837	Remarkably, species-specific expression dynamics are retained even when the key players governing a particular response are replaced by those from a species with a dissimilar response.
25691527	12	84	theme	availability	1899:1910	arg1	basis					1887:1891	the basis	1883:1891	the basis of CS availability and community composition	1883:1936	We establish that in the case of genes conferring the ability to utilize the nutrient chondroitin sulfate (CS), microbial species vary in how they temporally regulate these genes and exhibit subtle growth differences on the basis of CS availability and community composition.
25691527	6	85	theme	Bacteroides	904:914	arg1	species					916:922	Bacteroides species	904:922	Bacteroides species exhibiting distinct expression behaviors in the presence of CS	904:985	Bacteroides species exhibiting distinct expression behaviors in the presence of CS can be cocultured on CS.
25691527	4	86	theme	certain	555:561	arg1	species					575:581	certain Bacteroides species	555:581	certain Bacteroides species	555:581	Whereas certain Bacteroides species display a transient surge in CS utilization transcripts upon exposure to CS, other species exhibit sustained activation of these genes.
25691527	13	87	theme	gut	2053:2055	arg1	bacteria					2057:2064	gut bacteria	2053:2064	gut bacteria	2053:2064	Similarly to how differential regulation of orthologous genes enables related species to access new environments, gut bacteria may regulate the same genes in distinct fashions to reduce the overlap with coexisting species for utilization of available nutrients.
25691527	11	88	theme	remarkable	1621:1630	arg1	stability					1632:1640	such remarkable stability	1616:1640	such remarkable stability	1616:1640	How, then, does the gut microbial composition maintain such remarkable stability over long durations?
25691527	3	89	theme	orthologous	513:523	arg1	genes					540:544	orthologous CS utilization genes	513:544	orthologous CS utilization genes	513:544	Here we reveal that related Bacteroides species that can utilize the mammalian glycan chondroitin sulfate (CS) have diverged in the manner in which they temporally regulate orthologous CS utilization genes.
25691527	2	90	theme	nutrient	210:217	arg1	genes					231:235	homologous nutrient utilization genes	199:235	homologous nutrient utilization genes	199:235	Closely related species often harbor homologous nutrient utilization genes and cocolonize the gut, raising questions regarding the strategies mediating their stable coexistence.
25691527	0	91	theme	dynamic	17:23	arg1	responses					25:33	Species-specific dynamic responses	0:33	Species-specific dynamic responses of gut bacteria to a mammalian glycan	0:71	Species-specific dynamic responses of gut bacteria to a mammalian glycan.
24200877	0	0	theme	beta-glucan	97:107	arg1	system					156:161	beta-glucan and glucose sensing photometric flow injection system	97:161	system	156:161	Chitosan-guar gum-silver nanoparticles hybrid matrix with immobilized enzymes for fabrication of beta-glucan and glucose sensing photometric flow injection system.
24200877	7	1	theme	FIA	1090:1092	arg1	system					1101:1106	the nano-bio-composite FIA sensor system	1067:1106	the nano-bio-composite FIA sensor system for spiked and real β-(1→3)-d-glucan samples	1067:1151	The applicability of the nano-bio-composite FIA sensor system for spiked and real β-(1→3)-d-glucan samples were tested, and the accuracy of the results were greater than 95%.
24200877	8	2	theme	free	1276:1279	arg1	tool					1291:1294	a simple, interference free and rapid tool	1253:1294	a simple, interference free and rapid tool for monitoring glucose and β-glucan content, which can be used for various food samples with a little modification	1253:1409	Thus, the designed FIA provides a simple, interference free and rapid tool for monitoring glucose and β-glucan content, which can be used for various food samples with a little modification.
24200877	1	3	theme	fast	175:178	arg1	system					216:221	Simple and fast photometric flow injection analysis system	164:221	Simple and fast photometric flow injection analysis system	164:221	Simple and fast photometric flow injection analysis system was developed for sensing of β-1,3-glucan from medicinal mushroom Ganoderma lucidum during fermentation.
24200877	1	4	theme	mushroom	280:287	arg1	lucidum					299:305	medicinal mushroom Ganoderma lucidum	270:305	medicinal mushroom Ganoderma lucidum during fermentation	270:325	Simple and fast photometric flow injection analysis system was developed for sensing of β-1,3-glucan from medicinal mushroom Ganoderma lucidum during fermentation.
24200877	5	5	theme	reported	871:878	arg1	sensors					887:893	the previously reported glucan sensors	856:893	the previously reported glucan sensors	856:893	The analysis time of this FIA was approximately 40s, which is faster than the previously reported glucan sensors.
24200877	1	6	theme	photometric	180:190	arg1	system					216:221	Simple and fast photometric flow injection analysis system	164:221	Simple and fast photometric flow injection analysis system	164:221	Simple and fast photometric flow injection analysis system was developed for sensing of β-1,3-glucan from medicinal mushroom Ganoderma lucidum during fermentation.
24200877	1	7	theme	Ganoderma	289:297	arg1	lucidum					299:305	medicinal mushroom Ganoderma lucidum	270:305	medicinal mushroom Ganoderma lucidum during fermentation	270:325	Simple and fast photometric flow injection analysis system was developed for sensing of β-1,3-glucan from medicinal mushroom Ganoderma lucidum during fermentation.
24200877	0	8	theme	glucose	113:119	arg1	system					156:161	beta-glucan and glucose sensing photometric flow injection system	97:161	system	156:161	Chitosan-guar gum-silver nanoparticles hybrid matrix with immobilized enzymes for fabrication of beta-glucan and glucose sensing photometric flow injection system.
24200877	8	9	theme	β-glucan	1323:1330	arg1	content					1332:1338	β-glucan content	1323:1338	β-glucan content	1323:1338	Thus, the designed FIA provides a simple, interference free and rapid tool for monitoring glucose and β-glucan content, which can be used for various food samples with a little modification.
24200877	8	10	theme	designed	1231:1238	arg1	FIA					1240:1242	the designed FIA	1227:1242	the designed FIA	1227:1242	Thus, the designed FIA provides a simple, interference free and rapid tool for monitoring glucose and β-glucan content, which can be used for various food samples with a little modification.
24200877	3	11	theme	β-	601:602	arg1	biomarker					618:626	β-(1→3)-d-glucan biomarker	601:626	β-(1→3)-d-glucan biomarker	601:626	The bead packed mini-columns were then used to assemble a flow injection analysis (FIA) system for the detection of β-(1→3)-d-glucan biomarker or glucose.
24200877	3	12	theme	injection	548:556	arg1	analysis					558:565	flow injection analysis	543:565	a flow injection analysis (FIA) system for the detection of β-(1→3)-d-glucan biomarker or glucose	541:637	The bead packed mini-columns were then used to assemble a flow injection analysis (FIA) system for the detection of β-(1→3)-d-glucan biomarker or glucose.
24200877	3	12	theme	injection	548:556	arg1	FIA					568:570	FIA	568:570	FIA	568:570	The bead packed mini-columns were then used to assemble a flow injection analysis (FIA) system for the detection of β-(1→3)-d-glucan biomarker or glucose.
24200877	0	13	theme	photometric	129:139	arg1	system					156:161	beta-glucan and glucose sensing photometric flow injection system	97:161	system	156:161	Chitosan-guar gum-silver nanoparticles hybrid matrix with immobilized enzymes for fabrication of beta-glucan and glucose sensing photometric flow injection system.
24200877	6	14	theme	0.2-1.0μgmL	1003:1013	arg1	range					959:963	the range	955:963	the range of 0.25-1.25μgmL(-1) (R(2)=0.988) and 0.2-1.0μgmL(-1)(R(2)=0.979)	955:1029	The glucose and glucan calibration curves were obtained in the range of 0.25-1.25μgmL(-1) (R(2)=0.988) and 0.2-1.0μgmL(-1)(R(2)=0.979), respectively.
24200877	4	15	theme	colorimetric	645:656	arg1	system					663:668	This colorimetric flow system	640:668	This colorimetric flow system	640:668	This colorimetric flow system can detect glucose and glucan with detection limits as low as 50ngmL(-1) and 100ngmL(-1) (S/N=3), respectively.
24200877	5	16	theme	glucan	880:885	arg1	sensors					887:893	the previously reported glucan sensors	856:893	the previously reported glucan sensors	856:893	The analysis time of this FIA was approximately 40s, which is faster than the previously reported glucan sensors.
24200877	1	17	theme	flow	192:195	arg1	system					216:221	Simple and fast photometric flow injection analysis system	164:221	Simple and fast photometric flow injection analysis system	164:221	Simple and fast photometric flow injection analysis system was developed for sensing of β-1,3-glucan from medicinal mushroom Ganoderma lucidum during fermentation.
24200877	0	18	theme	sensing	121:127	arg1	system					156:161	beta-glucan and glucose sensing photometric flow injection system	97:161	system	156:161	Chitosan-guar gum-silver nanoparticles hybrid matrix with immobilized enzymes for fabrication of beta-glucan and glucose sensing photometric flow injection system.
24200877	8	19	used	used	1354:1357	arg2	glucose					1311:1317	glucose	1311:1317	glucose	1311:1317	Thus, the designed FIA provides a simple, interference free and rapid tool for monitoring glucose and β-glucan content, which can be used for various food samples with a little modification.
24200877	8	19	used	used	1354:1357	arg2	content					1332:1338	β-glucan content	1323:1338	β-glucan content	1323:1338	Thus, the designed FIA provides a simple, interference free and rapid tool for monitoring glucose and β-glucan content, which can be used for various food samples with a little modification.
24200877	1	20	theme	injection	197:205	arg1	system					216:221	Simple and fast photometric flow injection analysis system	164:221	Simple and fast photometric flow injection analysis system	164:221	Simple and fast photometric flow injection analysis system was developed for sensing of β-1,3-glucan from medicinal mushroom Ganoderma lucidum during fermentation.
24200877	6	21	theme	glucan	912:917	arg1	curves					931:936	The glucose and glucan calibration curves	896:936	curves	931:936	The glucose and glucan calibration curves were obtained in the range of 0.25-1.25μgmL(-1) (R(2)=0.988) and 0.2-1.0μgmL(-1)(R(2)=0.979), respectively.
24200877	7	22	theme	sensor	1094:1099	arg1	system					1101:1106	the nano-bio-composite FIA sensor system	1067:1106	the nano-bio-composite FIA sensor system for spiked and real β-(1→3)-d-glucan samples	1067:1151	The applicability of the nano-bio-composite FIA sensor system for spiked and real β-(1→3)-d-glucan samples were tested, and the accuracy of the results were greater than 95%.
24200877	7	23	theme	system	1101:1106	arg1	applicability					1050:1062	The applicability	1046:1062	The applicability of the nano-bio-composite FIA sensor system for spiked and real β-(1→3)-d-glucan samples	1046:1151	The applicability of the nano-bio-composite FIA sensor system for spiked and real β-(1→3)-d-glucan samples were tested, and the accuracy of the results were greater than 95%.
24200877	2	24	theme	chitosan-guar	350:362	arg1	beads					420:424	the chitosan-guar gum-silver nanoparticle-beta glucanase (Ch-GG-AgNPs-βG) beads	346:424	the chitosan-guar gum-silver nanoparticle-beta glucanase (Ch-GG-AgNPs-βG) beads	346:424	For this purpose, the chitosan-guar gum-silver nanoparticle-beta glucanase (Ch-GG-AgNPs-βG) beads and Ch-GG-AgNPs-GOD (glucose oxidase) beads were prepared.
24200877	1	25	theme	analysis	207:214	arg1	system					216:221	Simple and fast photometric flow injection analysis system	164:221	Simple and fast photometric flow injection analysis system	164:221	Simple and fast photometric flow injection analysis system was developed for sensing of β-1,3-glucan from medicinal mushroom Ganoderma lucidum during fermentation.
24200877	0	26	theme	Chitosan-guar	0:12	arg1	gum-silver					14:23	Chitosan-guar gum-silver	0:23	Chitosan-guar gum-silver	0:23	Chitosan-guar gum-silver nanoparticles hybrid matrix with immobilized enzymes for fabrication of beta-glucan and glucose sensing photometric flow injection system.
24200877	8	27	with	samples	1376:1382	arg1	modification					1398:1409	a little modification	1389:1409	a little modification	1389:1409	Thus, the designed FIA provides a simple, interference free and rapid tool for monitoring glucose and β-glucan content, which can be used for various food samples with a little modification.
24200877	2	28	theme	Ch-GG-AgNPs-GOD	430:444	arg1	beads					464:468	Ch-GG-AgNPs-GOD (glucose oxidase) beads	430:468	Ch-GG-AgNPs-GOD (glucose oxidase) beads	430:468	For this purpose, the chitosan-guar gum-silver nanoparticle-beta glucanase (Ch-GG-AgNPs-βG) beads and Ch-GG-AgNPs-GOD (glucose oxidase) beads were prepared.
24200877	0	29	theme	hybrid	39:44	arg1	matrix					46:51	hybrid matrix	39:51	hybrid matrix	39:51	Chitosan-guar gum-silver nanoparticles hybrid matrix with immobilized enzymes for fabrication of beta-glucan and glucose sensing photometric flow injection system.
24200877	0	30	theme	injection	146:154	arg1	system					156:161	beta-glucan and glucose sensing photometric flow injection system	97:161	system	156:161	Chitosan-guar gum-silver nanoparticles hybrid matrix with immobilized enzymes for fabrication of beta-glucan and glucose sensing photometric flow injection system.
24200877	3	31	used	used	524:527	arg2	mini-columns					501:512	The bead packed mini-columns	485:512	The bead packed mini-columns	485:512	The bead packed mini-columns were then used to assemble a flow injection analysis (FIA) system for the detection of β-(1→3)-d-glucan biomarker or glucose.
24200877	4	32	theme	flow	658:661	arg1	system					663:668	This colorimetric flow system	640:668	This colorimetric flow system	640:668	This colorimetric flow system can detect glucose and glucan with detection limits as low as 50ngmL(-1) and 100ngmL(-1) (S/N=3), respectively.
24200877	6	33	theme	calibration	919:929	arg1	curves					931:936	The glucose and glucan calibration curves	896:936	curves	931:936	The glucose and glucan calibration curves were obtained in the range of 0.25-1.25μgmL(-1) (R(2)=0.988) and 0.2-1.0μgmL(-1)(R(2)=0.979), respectively.
24200877	0	34	theme	flow	141:144	arg1	system					156:161	beta-glucan and glucose sensing photometric flow injection system	97:161	system	156:161	Chitosan-guar gum-silver nanoparticles hybrid matrix with immobilized enzymes for fabrication of beta-glucan and glucose sensing photometric flow injection system.
24200877	4	35	with	glucan	693:698	arg1	limits					715:720	detection limits	705:720	detection limits	705:720	This colorimetric flow system can detect glucose and glucan with detection limits as low as 50ngmL(-1) and 100ngmL(-1) (S/N=3), respectively.
24200877	2	36	theme	Ch-GG-AgNPs-βG	404:417	arg1	beads					420:424	the chitosan-guar gum-silver nanoparticle-beta glucanase (Ch-GG-AgNPs-βG) beads	346:424	the chitosan-guar gum-silver nanoparticle-beta glucanase (Ch-GG-AgNPs-βG) beads	346:424	For this purpose, the chitosan-guar gum-silver nanoparticle-beta glucanase (Ch-GG-AgNPs-βG) beads and Ch-GG-AgNPs-GOD (glucose oxidase) beads were prepared.
24200877	8	37	theme	food	1371:1374	arg1	samples					1376:1382	various food samples	1363:1382	various food samples with a little modification	1363:1409	Thus, the designed FIA provides a simple, interference free and rapid tool for monitoring glucose and β-glucan content, which can be used for various food samples with a little modification.
24200877	4	38	theme	detection	705:713	arg1	limits					715:720	detection limits	705:720	detection limits	705:720	This colorimetric flow system can detect glucose and glucan with detection limits as low as 50ngmL(-1) and 100ngmL(-1) (S/N=3), respectively.
24200877	3	39	theme	flow	543:546	arg1	analysis					558:565	flow injection analysis	543:565	a flow injection analysis (FIA) system for the detection of β-(1→3)-d-glucan biomarker or glucose	541:637	The bead packed mini-columns were then used to assemble a flow injection analysis (FIA) system for the detection of β-(1→3)-d-glucan biomarker or glucose.
24200877	3	39	theme	flow	543:546	arg1	FIA					568:570	FIA	568:570	FIA	568:570	The bead packed mini-columns were then used to assemble a flow injection analysis (FIA) system for the detection of β-(1→3)-d-glucan biomarker or glucose.
24200877	7	40	theme	real	1123:1126	arg1	1→3					1131:1133	1→3	1131:1133	1→3	1131:1133	The applicability of the nano-bio-composite FIA sensor system for spiked and real β-(1→3)-d-glucan samples were tested, and the accuracy of the results were greater than 95%.
24200877	7	40	theme	real	1123:1126	arg1	β-					1128:1129	real β-	1123:1129	real β-(1→3)-d-glucan samples	1123:1151	The applicability of the nano-bio-composite FIA sensor system for spiked and real β-(1→3)-d-glucan samples were tested, and the accuracy of the results were greater than 95%.
24200877	3	41	theme	-d-glucan	608:616	arg1	biomarker					618:626	β-(1→3)-d-glucan biomarker	601:626	β-(1→3)-d-glucan biomarker	601:626	The bead packed mini-columns were then used to assemble a flow injection analysis (FIA) system for the detection of β-(1→3)-d-glucan biomarker or glucose.
24200877	5	42	theme	FIA	808:810	arg1	40s					830:832	40s	830:832	40s	830:832	The analysis time of this FIA was approximately 40s, which is faster than the previously reported glucan sensors.
24200877	5	42	theme	FIA	808:810	arg1	time					795:798	The analysis time	782:798	The analysis time of this FIA	782:810	The analysis time of this FIA was approximately 40s, which is faster than the previously reported glucan sensors.
24200877	3	43	theme	bead	489:492	arg1	mini-columns					501:512	The bead packed mini-columns	485:512	The bead packed mini-columns	485:512	The bead packed mini-columns were then used to assemble a flow injection analysis (FIA) system for the detection of β-(1→3)-d-glucan biomarker or glucose.
24200877	0	44	theme	system	156:161	arg1	fabrication					82:92	fabrication	82:92	fabrication of beta-glucan and glucose sensing photometric flow injection system	82:161	Chitosan-guar gum-silver nanoparticles hybrid matrix with immobilized enzymes for fabrication of beta-glucan and glucose sensing photometric flow injection system.
24200877	4	45	with	glucose	681:687	arg1	limits					715:720	detection limits	705:720	detection limits	705:720	This colorimetric flow system can detect glucose and glucan with detection limits as low as 50ngmL(-1) and 100ngmL(-1) (S/N=3), respectively.
24200877	3	46	theme	biomarker	618:626	arg1	detection					588:596	the detection	584:596	the detection of β-(1→3)-d-glucan biomarker or glucose	584:637	The bead packed mini-columns were then used to assemble a flow injection analysis (FIA) system for the detection of β-(1→3)-d-glucan biomarker or glucose.
24200877	5	47	theme	analysis	786:793	arg1	40s					830:832	40s	830:832	40s	830:832	The analysis time of this FIA was approximately 40s, which is faster than the previously reported glucan sensors.
24200877	5	47	theme	analysis	786:793	arg1	time					795:798	The analysis time	782:798	The analysis time of this FIA	782:810	The analysis time of this FIA was approximately 40s, which is faster than the previously reported glucan sensors.
24200877	7	48	theme	nano-bio-composite	1071:1088	arg1	system					1101:1106	the nano-bio-composite FIA sensor system	1067:1106	the nano-bio-composite FIA sensor system for spiked and real β-(1→3)-d-glucan samples	1067:1151	The applicability of the nano-bio-composite FIA sensor system for spiked and real β-(1→3)-d-glucan samples were tested, and the accuracy of the results were greater than 95%.
24200877	3	49	theme	1→3	604:606	arg1	biomarker					618:626	β-(1→3)-d-glucan biomarker	601:626	β-(1→3)-d-glucan biomarker	601:626	The bead packed mini-columns were then used to assemble a flow injection analysis (FIA) system for the detection of β-(1→3)-d-glucan biomarker or glucose.
24200877	1	50	from	sensing	241:247	arg1	lucidum					299:305	medicinal mushroom Ganoderma lucidum	270:305	medicinal mushroom Ganoderma lucidum during fermentation	270:325	Simple and fast photometric flow injection analysis system was developed for sensing of β-1,3-glucan from medicinal mushroom Ganoderma lucidum during fermentation.
24200877	0	51	theme	immobilized	58:68	arg1	enzymes					70:76	immobilized enzymes	58:76	immobilized enzymes for fabrication of beta-glucan and glucose sensing photometric flow injection system	58:161	Chitosan-guar gum-silver nanoparticles hybrid matrix with immobilized enzymes for fabrication of beta-glucan and glucose sensing photometric flow injection system.
24200877	8	52	theme	little	1391:1396	arg1	modification					1398:1409	a little modification	1389:1409	a little modification	1389:1409	Thus, the designed FIA provides a simple, interference free and rapid tool for monitoring glucose and β-glucan content, which can be used for various food samples with a little modification.
24200877	1	53	from	lucidum	299:305	arg1	β-1,3-glucan					252:263	β-1,3-glucan	252:263	β-1,3-glucan from medicinal mushroom Ganoderma lucidum during fermentation	252:325	Simple and fast photometric flow injection analysis system was developed for sensing of β-1,3-glucan from medicinal mushroom Ganoderma lucidum during fermentation.
24200877	1	53	from	lucidum	299:305	arg1	sensing					241:247	sensing	241:247	sensing of β-1,3-glucan from medicinal mushroom Ganoderma lucidum during fermentation	241:325	Simple and fast photometric flow injection analysis system was developed for sensing of β-1,3-glucan from medicinal mushroom Ganoderma lucidum during fermentation.
24200877	8	54	theme	various	1363:1369	arg1	samples					1376:1382	various food samples	1363:1382	various food samples with a little modification	1363:1409	Thus, the designed FIA provides a simple, interference free and rapid tool for monitoring glucose and β-glucan content, which can be used for various food samples with a little modification.
24200877	3	55	theme	analysis	558:565	arg1	system					573:578	a flow injection analysis (FIA) system	541:578	a flow injection analysis (FIA) system for the detection of β-(1→3)-d-glucan biomarker or glucose	541:637	The bead packed mini-columns were then used to assemble a flow injection analysis (FIA) system for the detection of β-(1→3)-d-glucan biomarker or glucose.
24200877	2	56	theme	glucanase	393:401	arg1	beads					420:424	the chitosan-guar gum-silver nanoparticle-beta glucanase (Ch-GG-AgNPs-βG) beads	346:424	the chitosan-guar gum-silver nanoparticle-beta glucanase (Ch-GG-AgNPs-βG) beads	346:424	For this purpose, the chitosan-guar gum-silver nanoparticle-beta glucanase (Ch-GG-AgNPs-βG) beads and Ch-GG-AgNPs-GOD (glucose oxidase) beads were prepared.
24200877	7	57	theme	results	1190:1196	arg1	%					1218:1218	greater than 95%	1203:1218	greater than 95%	1203:1218	The applicability of the nano-bio-composite FIA sensor system for spiked and real β-(1→3)-d-glucan samples were tested, and the accuracy of the results were greater than 95%.
24200877	7	57	theme	results	1190:1196	arg1	accuracy					1174:1181	the accuracy	1170:1181	the accuracy of the results	1170:1196	The applicability of the nano-bio-composite FIA sensor system for spiked and real β-(1→3)-d-glucan samples were tested, and the accuracy of the results were greater than 95%.
24200877	8	58	theme	rapid	1285:1289	arg1	tool					1291:1294	a simple, interference free and rapid tool	1253:1294	a simple, interference free and rapid tool for monitoring glucose and β-glucan content, which can be used for various food samples with a little modification	1253:1409	Thus, the designed FIA provides a simple, interference free and rapid tool for monitoring glucose and β-glucan content, which can be used for various food samples with a little modification.
24200877	2	59	theme	nanoparticle-beta	375:391	arg1	beads					420:424	the chitosan-guar gum-silver nanoparticle-beta glucanase (Ch-GG-AgNPs-βG) beads	346:424	the chitosan-guar gum-silver nanoparticle-beta glucanase (Ch-GG-AgNPs-βG) beads	346:424	For this purpose, the chitosan-guar gum-silver nanoparticle-beta glucanase (Ch-GG-AgNPs-βG) beads and Ch-GG-AgNPs-GOD (glucose oxidase) beads were prepared.
24200877	7	60	theme	-d-glucan	1135:1143	arg1	samples					1145:1151	real β-(1→3)-d-glucan samples	1123:1151	real β-(1→3)-d-glucan samples	1123:1151	The applicability of the nano-bio-composite FIA sensor system for spiked and real β-(1→3)-d-glucan samples were tested, and the accuracy of the results were greater than 95%.
24200877	2	61	theme	gum-silver	364:373	arg1	beads					420:424	the chitosan-guar gum-silver nanoparticle-beta glucanase (Ch-GG-AgNPs-βG) beads	346:424	the chitosan-guar gum-silver nanoparticle-beta glucanase (Ch-GG-AgNPs-βG) beads	346:424	For this purpose, the chitosan-guar gum-silver nanoparticle-beta glucanase (Ch-GG-AgNPs-βG) beads and Ch-GG-AgNPs-GOD (glucose oxidase) beads were prepared.
24200877	1	62	theme	β-1,3-glucan	252:263	arg1	sensing					241:247	sensing	241:247	sensing of β-1,3-glucan from medicinal mushroom Ganoderma lucidum during fermentation	241:325	Simple and fast photometric flow injection analysis system was developed for sensing of β-1,3-glucan from medicinal mushroom Ganoderma lucidum during fermentation.
24200877	3	63	theme	packed	494:499	arg1	mini-columns					501:512	The bead packed mini-columns	485:512	The bead packed mini-columns	485:512	The bead packed mini-columns were then used to assemble a flow injection analysis (FIA) system for the detection of β-(1→3)-d-glucan biomarker or glucose.
24200877	8	64	theme	simple	1255:1260	arg1	tool					1291:1294	a simple, interference free and rapid tool	1253:1294	a simple, interference free and rapid tool for monitoring glucose and β-glucan content, which can be used for various food samples with a little modification	1253:1409	Thus, the designed FIA provides a simple, interference free and rapid tool for monitoring glucose and β-glucan content, which can be used for various food samples with a little modification.
24200877	1	65	theme	Simple	164:169	arg1	system					216:221	Simple and fast photometric flow injection analysis system	164:221	Simple and fast photometric flow injection analysis system	164:221	Simple and fast photometric flow injection analysis system was developed for sensing of β-1,3-glucan from medicinal mushroom Ganoderma lucidum during fermentation.
24200877	2	66	theme	glucose	447:453	arg1	Ch-GG-AgNPs-GOD					430:444	Ch-GG-AgNPs-GOD	430:444	Ch-GG-AgNPs-GOD (glucose oxidase) beads	430:468	For this purpose, the chitosan-guar gum-silver nanoparticle-beta glucanase (Ch-GG-AgNPs-βG) beads and Ch-GG-AgNPs-GOD (glucose oxidase) beads were prepared.
24200877	2	66	theme	glucose	447:453	arg1	oxidase					455:461	glucose oxidase	447:461	glucose oxidase	447:461	For this purpose, the chitosan-guar gum-silver nanoparticle-beta glucanase (Ch-GG-AgNPs-βG) beads and Ch-GG-AgNPs-GOD (glucose oxidase) beads were prepared.
24200877	6	67	theme	0.25-1.25μgmL	968:980	arg1	range					959:963	the range	955:963	the range of 0.25-1.25μgmL(-1) (R(2)=0.988) and 0.2-1.0μgmL(-1)(R(2)=0.979)	955:1029	The glucose and glucan calibration curves were obtained in the range of 0.25-1.25μgmL(-1) (R(2)=0.988) and 0.2-1.0μgmL(-1)(R(2)=0.979), respectively.
24200877	3	68	theme	glucose	631:637	arg1	detection					588:596	the detection	584:596	the detection of β-(1→3)-d-glucan biomarker or glucose	584:637	The bead packed mini-columns were then used to assemble a flow injection analysis (FIA) system for the detection of β-(1→3)-d-glucan biomarker or glucose.
24200877	7	69	theme	β-	1128:1129	arg1	samples					1145:1151	real β-(1→3)-d-glucan samples	1123:1151	real β-(1→3)-d-glucan samples	1123:1151	The applicability of the nano-bio-composite FIA sensor system for spiked and real β-(1→3)-d-glucan samples were tested, and the accuracy of the results were greater than 95%.
24200877	1	70	theme	medicinal	270:278	arg1	lucidum					299:305	medicinal mushroom Ganoderma lucidum	270:305	medicinal mushroom Ganoderma lucidum during fermentation	270:325	Simple and fast photometric flow injection analysis system was developed for sensing of β-1,3-glucan from medicinal mushroom Ganoderma lucidum during fermentation.
27267571	6	0	with	carriers	1128:1135	arg1	potential					1148:1156	great potential	1142:1156	great potential for medical applications	1142:1181	Therefore, CKGM/HACC nanospheres are novel drug delivery carriers with great potential for medical applications.
27267571	5	1	dep	in	1005:1006	arg1	vitro					1008:1012	vitro	1008:1012	vitro	1008:1012	Under optimum conditions, the OVA encapsulation efficiency of CKGM/HACC nanospheres was 71.8%, while sustained and continuous in vitro OVA release over a period of more than 24h was observed.
27267571	6	2	theme	drug	1114:1117	arg1	carriers					1128:1135	novel drug delivery carriers	1108:1135	novel drug delivery carriers with great potential for medical applications	1108:1181	Therefore, CKGM/HACC nanospheres are novel drug delivery carriers with great potential for medical applications.
27267571	6	2	theme	drug	1114:1117	arg1	nanospheres					1092:1102	CKGM/HACC nanospheres	1082:1102	CKGM/HACC nanospheres	1082:1102	Therefore, CKGM/HACC nanospheres are novel drug delivery carriers with great potential for medical applications.
27267571	2	3	theme	delivery	378:385	arg1	vehicle					387:393	a vaccine delivery vehicle	368:393	a vaccine delivery vehicle	368:393	In the present study, novel composite nanospheres were prepared from carboxymethyl konjac glucomannan (CKGM) and 2-hydroxypropyl trimethyl ammonium chloride chitosan (HACC) as a vaccine delivery vehicle by electrostatic complexation in a neutral aqueous solution without the use of chemical crosslinkers.
27267571	2	3	theme	delivery	378:385	arg1	nanospheres					230:240	novel composite nanospheres	214:240	novel composite nanospheres	214:240	In the present study, novel composite nanospheres were prepared from carboxymethyl konjac glucomannan (CKGM) and 2-hydroxypropyl trimethyl ammonium chloride chitosan (HACC) as a vaccine delivery vehicle by electrostatic complexation in a neutral aqueous solution without the use of chemical crosslinkers.
27267571	5	4	theme	encapsulation	913:925	arg1	efficiency					927:936	the OVA encapsulation efficiency	905:936	the OVA encapsulation efficiency of CKGM/HACC nanospheres	905:961	Under optimum conditions, the OVA encapsulation efficiency of CKGM/HACC nanospheres was 71.8%, while sustained and continuous in vitro OVA release over a period of more than 24h was observed.
27267571	5	4	theme	encapsulation	913:925	arg1	%					971:971	71.8%	967:971	71.8%	967:971	Under optimum conditions, the OVA encapsulation efficiency of CKGM/HACC nanospheres was 71.8%, while sustained and continuous in vitro OVA release over a period of more than 24h was observed.
27267571	6	5	theme	novel	1108:1112	arg1	carriers					1128:1135	novel drug delivery carriers	1108:1135	novel drug delivery carriers with great potential for medical applications	1108:1181	Therefore, CKGM/HACC nanospheres are novel drug delivery carriers with great potential for medical applications.
27267571	6	5	theme	novel	1108:1112	arg1	nanospheres					1092:1102	CKGM/HACC nanospheres	1082:1102	CKGM/HACC nanospheres	1082:1102	Therefore, CKGM/HACC nanospheres are novel drug delivery carriers with great potential for medical applications.
27267571	3	6	theme	average	547:553	arg1	size					574:577	the average CKGM/HACC particle size	543:577	the average CKGM/HACC particle size	543:577	By altering the CKGM and HACC concentrations, the average CKGM/HACC particle size could be tuned from approximately 600nm to 1460nm and the zeta potential from 39mV to 50mV.
27267571	3	6	theme	average	547:553	arg1	tuned					588:592	tuned	588:592	tuned	588:592	By altering the CKGM and HACC concentrations, the average CKGM/HACC particle size could be tuned from approximately 600nm to 1460nm and the zeta potential from 39mV to 50mV.
27267571	2	7	theme	ammonium	331:338	arg1	chloride					340:347	2-hydroxypropyl trimethyl ammonium chloride	305:347	2-hydroxypropyl trimethyl ammonium chloride chitosan (HACC)	305:363	In the present study, novel composite nanospheres were prepared from carboxymethyl konjac glucomannan (CKGM) and 2-hydroxypropyl trimethyl ammonium chloride chitosan (HACC) as a vaccine delivery vehicle by electrostatic complexation in a neutral aqueous solution without the use of chemical crosslinkers.
27267571	5	8	theme	sustained	980:988	arg1	release					1018:1024	sustained and continuous in vitro OVA release	980:1024	sustained and continuous in vitro OVA release over a period of more than 24h	980:1055	Under optimum conditions, the OVA encapsulation efficiency of CKGM/HACC nanospheres was 71.8%, while sustained and continuous in vitro OVA release over a period of more than 24h was observed.
27267571	4	9	theme	various	740:746	arg1	parameters					748:757	various parameters	740:757	various parameters	740:757	Furthermore, using ovalbumin (OVA) as a model molecule for vaccines, various parameters were determined to affect the CKGM/HACC nanosphere encapsulation efficiency and in vitro controlled release properties.
27267571	5	10	theme	continuous	994:1003	arg1	release					1018:1024	sustained and continuous in vitro OVA release	980:1024	sustained and continuous in vitro OVA release over a period of more than 24h	980:1055	Under optimum conditions, the OVA encapsulation efficiency of CKGM/HACC nanospheres was 71.8%, while sustained and continuous in vitro OVA release over a period of more than 24h was observed.
27267571	2	11	theme	trimethyl	321:329	arg1	chloride					340:347	2-hydroxypropyl trimethyl ammonium chloride	305:347	2-hydroxypropyl trimethyl ammonium chloride chitosan (HACC)	305:363	In the present study, novel composite nanospheres were prepared from carboxymethyl konjac glucomannan (CKGM) and 2-hydroxypropyl trimethyl ammonium chloride chitosan (HACC) as a vaccine delivery vehicle by electrostatic complexation in a neutral aqueous solution without the use of chemical crosslinkers.
27267571	3	12	from	39mV	657:660	arg1	potential					642:650	the zeta potential	633:650	the zeta potential from 39mV to 50mV	633:668	By altering the CKGM and HACC concentrations, the average CKGM/HACC particle size could be tuned from approximately 600nm to 1460nm and the zeta potential from 39mV to 50mV.
27267571	3	12	from	39mV	657:660	arg1	1460nm					622:627	1460nm	622:627	1460nm	622:627	By altering the CKGM and HACC concentrations, the average CKGM/HACC particle size could be tuned from approximately 600nm to 1460nm and the zeta potential from 39mV to 50mV.
27267571	2	13	theme	vaccine	370:376	arg1	vehicle					387:393	a vaccine delivery vehicle	368:393	a vaccine delivery vehicle	368:393	In the present study, novel composite nanospheres were prepared from carboxymethyl konjac glucomannan (CKGM) and 2-hydroxypropyl trimethyl ammonium chloride chitosan (HACC) as a vaccine delivery vehicle by electrostatic complexation in a neutral aqueous solution without the use of chemical crosslinkers.
27267571	2	13	theme	vaccine	370:376	arg1	nanospheres					230:240	novel composite nanospheres	214:240	novel composite nanospheres	214:240	In the present study, novel composite nanospheres were prepared from carboxymethyl konjac glucomannan (CKGM) and 2-hydroxypropyl trimethyl ammonium chloride chitosan (HACC) as a vaccine delivery vehicle by electrostatic complexation in a neutral aqueous solution without the use of chemical crosslinkers.
27267571	1	14	theme	Biodegradable	89:101	arg1	nanospheres					129:139	Biodegradable and biocompatible polymer nanospheres	89:139	Biodegradable and biocompatible polymer nanospheres	89:139	Biodegradable and biocompatible polymer nanospheres are useful materials for controlled drug delivery.
27267571	1	14	theme	Biodegradable	89:101	arg1	materials					152:160	useful materials	145:160	useful materials for controlled drug delivery	145:189	Biodegradable and biocompatible polymer nanospheres are useful materials for controlled drug delivery.
27267571	2	15	theme	neutral	430:436	arg1	solution					446:453	a neutral aqueous solution	428:453	a neutral aqueous solution without the use of chemical crosslinkers	428:494	In the present study, novel composite nanospheres were prepared from carboxymethyl konjac glucomannan (CKGM) and 2-hydroxypropyl trimethyl ammonium chloride chitosan (HACC) as a vaccine delivery vehicle by electrostatic complexation in a neutral aqueous solution without the use of chemical crosslinkers.
27267571	4	16	theme	release	859:865	arg1	properties					867:876	in vitro controlled release properties	839:876	in vitro controlled release properties	839:876	Furthermore, using ovalbumin (OVA) as a model molecule for vaccines, various parameters were determined to affect the CKGM/HACC nanosphere encapsulation efficiency and in vitro controlled release properties.
27267571	3	17	theme	HACC	522:525	arg1	concentrations					527:540	the CKGM and HACC concentrations	509:540	the CKGM and HACC concentrations	509:540	By altering the CKGM and HACC concentrations, the average CKGM/HACC particle size could be tuned from approximately 600nm to 1460nm and the zeta potential from 39mV to 50mV.
27267571	6	18	theme	CKGM/HACC	1082:1090	arg1	carriers					1128:1135	novel drug delivery carriers	1108:1135	novel drug delivery carriers with great potential for medical applications	1108:1181	Therefore, CKGM/HACC nanospheres are novel drug delivery carriers with great potential for medical applications.
27267571	6	18	theme	CKGM/HACC	1082:1090	arg1	nanospheres					1092:1102	CKGM/HACC nanospheres	1082:1102	CKGM/HACC nanospheres	1082:1102	Therefore, CKGM/HACC nanospheres are novel drug delivery carriers with great potential for medical applications.
27267571	2	19	theme	crosslinkers	483:494	arg1	use					467:469	the use	463:469	the use of chemical crosslinkers	463:494	In the present study, novel composite nanospheres were prepared from carboxymethyl konjac glucomannan (CKGM) and 2-hydroxypropyl trimethyl ammonium chloride chitosan (HACC) as a vaccine delivery vehicle by electrostatic complexation in a neutral aqueous solution without the use of chemical crosslinkers.
27267571	1	20	theme	biocompatible	107:119	arg1	nanospheres					129:139	Biodegradable and biocompatible polymer nanospheres	89:139	Biodegradable and biocompatible polymer nanospheres	89:139	Biodegradable and biocompatible polymer nanospheres are useful materials for controlled drug delivery.
27267571	1	20	theme	biocompatible	107:119	arg1	materials					152:160	useful materials	145:160	useful materials for controlled drug delivery	145:189	Biodegradable and biocompatible polymer nanospheres are useful materials for controlled drug delivery.
27267571	3	21	theme	particle	565:572	arg1	size					574:577	the average CKGM/HACC particle size	543:577	the average CKGM/HACC particle size	543:577	By altering the CKGM and HACC concentrations, the average CKGM/HACC particle size could be tuned from approximately 600nm to 1460nm and the zeta potential from 39mV to 50mV.
27267571	3	21	theme	particle	565:572	arg1	tuned					588:592	tuned	588:592	tuned	588:592	By altering the CKGM and HACC concentrations, the average CKGM/HACC particle size could be tuned from approximately 600nm to 1460nm and the zeta potential from 39mV to 50mV.
27267571	2	22	theme	chemical	474:481	arg1	crosslinkers					483:494	chemical crosslinkers	474:494	chemical crosslinkers	474:494	In the present study, novel composite nanospheres were prepared from carboxymethyl konjac glucomannan (CKGM) and 2-hydroxypropyl trimethyl ammonium chloride chitosan (HACC) as a vaccine delivery vehicle by electrostatic complexation in a neutral aqueous solution without the use of chemical crosslinkers.
27267571	1	23	theme	polymer	121:127	arg1	nanospheres					129:139	Biodegradable and biocompatible polymer nanospheres	89:139	Biodegradable and biocompatible polymer nanospheres	89:139	Biodegradable and biocompatible polymer nanospheres are useful materials for controlled drug delivery.
27267571	1	23	theme	polymer	121:127	arg1	materials					152:160	useful materials	145:160	useful materials for controlled drug delivery	145:189	Biodegradable and biocompatible polymer nanospheres are useful materials for controlled drug delivery.
27267571	5	24	theme	CKGM/HACC	941:949	arg1	nanospheres					951:961	CKGM/HACC nanospheres	941:961	CKGM/HACC nanospheres	941:961	Under optimum conditions, the OVA encapsulation efficiency of CKGM/HACC nanospheres was 71.8%, while sustained and continuous in vitro OVA release over a period of more than 24h was observed.
27267571	6	25	theme	medical	1162:1168	arg1	applications					1170:1181	medical applications	1162:1181	medical applications	1162:1181	Therefore, CKGM/HACC nanospheres are novel drug delivery carriers with great potential for medical applications.
27267571	4	26	theme	in	839:840	arg1	properties					867:876	in vitro controlled release properties	839:876	in vitro controlled release properties	839:876	Furthermore, using ovalbumin (OVA) as a model molecule for vaccines, various parameters were determined to affect the CKGM/HACC nanosphere encapsulation efficiency and in vitro controlled release properties.
27267571	0	27	theme	sustainable	16:26	arg1	release					28:34	sustainable release	16:34	sustainable release	16:34	Preparation and sustainable release of modified konjac glucomannan/chitosan nanospheres.
27267571	2	28	theme	2-hydroxypropyl	305:319	arg1	chloride					340:347	2-hydroxypropyl trimethyl ammonium chloride	305:347	2-hydroxypropyl trimethyl ammonium chloride chitosan (HACC)	305:363	In the present study, novel composite nanospheres were prepared from carboxymethyl konjac glucomannan (CKGM) and 2-hydroxypropyl trimethyl ammonium chloride chitosan (HACC) as a vaccine delivery vehicle by electrostatic complexation in a neutral aqueous solution without the use of chemical crosslinkers.
27267571	2	29	theme	chloride	340:347	arg1	chitosan					349:356	2-hydroxypropyl trimethyl ammonium chloride chitosan	305:356	2-hydroxypropyl trimethyl ammonium chloride chitosan (HACC)	305:363	In the present study, novel composite nanospheres were prepared from carboxymethyl konjac glucomannan (CKGM) and 2-hydroxypropyl trimethyl ammonium chloride chitosan (HACC) as a vaccine delivery vehicle by electrostatic complexation in a neutral aqueous solution without the use of chemical crosslinkers.
27267571	2	29	theme	chloride	340:347	arg1	HACC					359:362	HACC	359:362	HACC	359:362	In the present study, novel composite nanospheres were prepared from carboxymethyl konjac glucomannan (CKGM) and 2-hydroxypropyl trimethyl ammonium chloride chitosan (HACC) as a vaccine delivery vehicle by electrostatic complexation in a neutral aqueous solution without the use of chemical crosslinkers.
27267571	5	30	theme	more	1043:1046	arg1	period					1033:1038	a period	1031:1038	a period of more than 24h	1031:1055	Under optimum conditions, the OVA encapsulation efficiency of CKGM/HACC nanospheres was 71.8%, while sustained and continuous in vitro OVA release over a period of more than 24h was observed.
27267571	1	31	theme	drug	177:180	arg1	delivery					182:189	controlled drug delivery	166:189	controlled drug delivery	166:189	Biodegradable and biocompatible polymer nanospheres are useful materials for controlled drug delivery.
27267571	0	32	theme	modified	39:46	arg1	glucomannan/chitosan					55:74	modified konjac glucomannan/chitosan	39:74	modified konjac glucomannan/chitosan	39:74	Preparation and sustainable release of modified konjac glucomannan/chitosan nanospheres.
27267571	5	33	theme	nanospheres	951:961	arg1	efficiency					927:936	the OVA encapsulation efficiency	905:936	the OVA encapsulation efficiency of CKGM/HACC nanospheres	905:961	Under optimum conditions, the OVA encapsulation efficiency of CKGM/HACC nanospheres was 71.8%, while sustained and continuous in vitro OVA release over a period of more than 24h was observed.
27267571	5	33	theme	nanospheres	951:961	arg1	%					971:971	71.8%	967:971	71.8%	967:971	Under optimum conditions, the OVA encapsulation efficiency of CKGM/HACC nanospheres was 71.8%, while sustained and continuous in vitro OVA release over a period of more than 24h was observed.
27267571	6	34	theme	great	1142:1146	arg1	potential					1148:1156	great potential	1142:1156	great potential for medical applications	1142:1181	Therefore, CKGM/HACC nanospheres are novel drug delivery carriers with great potential for medical applications.
27267571	2	35	theme	konjac	275:280	arg1	CKGM					295:298	CKGM	295:298	CKGM	295:298	In the present study, novel composite nanospheres were prepared from carboxymethyl konjac glucomannan (CKGM) and 2-hydroxypropyl trimethyl ammonium chloride chitosan (HACC) as a vaccine delivery vehicle by electrostatic complexation in a neutral aqueous solution without the use of chemical crosslinkers.
27267571	2	35	theme	konjac	275:280	arg1	glucomannan					282:292	carboxymethyl konjac glucomannan	261:292	carboxymethyl konjac glucomannan (CKGM)	261:299	In the present study, novel composite nanospheres were prepared from carboxymethyl konjac glucomannan (CKGM) and 2-hydroxypropyl trimethyl ammonium chloride chitosan (HACC) as a vaccine delivery vehicle by electrostatic complexation in a neutral aqueous solution without the use of chemical crosslinkers.
27267571	4	36	dep	in	839:840	arg1	vitro					842:846	vitro	842:846	vitro	842:846	Furthermore, using ovalbumin (OVA) as a model molecule for vaccines, various parameters were determined to affect the CKGM/HACC nanosphere encapsulation efficiency and in vitro controlled release properties.
27267571	5	37	theme	optimum	885:891	arg1	conditions					893:902	optimum conditions	885:902	optimum conditions	885:902	Under optimum conditions, the OVA encapsulation efficiency of CKGM/HACC nanospheres was 71.8%, while sustained and continuous in vitro OVA release over a period of more than 24h was observed.
27267571	2	38	theme	present	199:205	arg1	study					207:211	the present study	195:211	the present study	195:211	In the present study, novel composite nanospheres were prepared from carboxymethyl konjac glucomannan (CKGM) and 2-hydroxypropyl trimethyl ammonium chloride chitosan (HACC) as a vaccine delivery vehicle by electrostatic complexation in a neutral aqueous solution without the use of chemical crosslinkers.
27267571	2	39	theme	carboxymethyl	261:273	arg1	CKGM					295:298	CKGM	295:298	CKGM	295:298	In the present study, novel composite nanospheres were prepared from carboxymethyl konjac glucomannan (CKGM) and 2-hydroxypropyl trimethyl ammonium chloride chitosan (HACC) as a vaccine delivery vehicle by electrostatic complexation in a neutral aqueous solution without the use of chemical crosslinkers.
27267571	2	39	theme	carboxymethyl	261:273	arg1	glucomannan					282:292	carboxymethyl konjac glucomannan	261:292	carboxymethyl konjac glucomannan (CKGM)	261:299	In the present study, novel composite nanospheres were prepared from carboxymethyl konjac glucomannan (CKGM) and 2-hydroxypropyl trimethyl ammonium chloride chitosan (HACC) as a vaccine delivery vehicle by electrostatic complexation in a neutral aqueous solution without the use of chemical crosslinkers.
27267571	2	40	theme	aqueous	438:444	arg1	solution					446:453	a neutral aqueous solution	428:453	a neutral aqueous solution without the use of chemical crosslinkers	428:494	In the present study, novel composite nanospheres were prepared from carboxymethyl konjac glucomannan (CKGM) and 2-hydroxypropyl trimethyl ammonium chloride chitosan (HACC) as a vaccine delivery vehicle by electrostatic complexation in a neutral aqueous solution without the use of chemical crosslinkers.
27267571	0	41	theme	glucomannan/chitosan	55:74	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and sustainable release of modified konjac glucomannan/chitosan nanospheres.
27267571	0	41	theme	glucomannan/chitosan	55:74	arg1	release					28:34	sustainable release	16:34	sustainable release	16:34	Preparation and sustainable release of modified konjac glucomannan/chitosan nanospheres.
27267571	5	42	theme	OVA	909:911	arg1	efficiency					927:936	the OVA encapsulation efficiency	905:936	the OVA encapsulation efficiency of CKGM/HACC nanospheres	905:961	Under optimum conditions, the OVA encapsulation efficiency of CKGM/HACC nanospheres was 71.8%, while sustained and continuous in vitro OVA release over a period of more than 24h was observed.
27267571	5	42	theme	OVA	909:911	arg1	%					971:971	71.8%	967:971	71.8%	967:971	Under optimum conditions, the OVA encapsulation efficiency of CKGM/HACC nanospheres was 71.8%, while sustained and continuous in vitro OVA release over a period of more than 24h was observed.
27267571	5	43	theme	in	1005:1006	arg1	release					1018:1024	sustained and continuous in vitro OVA release	980:1024	sustained and continuous in vitro OVA release over a period of more than 24h	980:1055	Under optimum conditions, the OVA encapsulation efficiency of CKGM/HACC nanospheres was 71.8%, while sustained and continuous in vitro OVA release over a period of more than 24h was observed.
27267571	4	44	theme	controlled	848:857	arg1	properties					867:876	in vitro controlled release properties	839:876	in vitro controlled release properties	839:876	Furthermore, using ovalbumin (OVA) as a model molecule for vaccines, various parameters were determined to affect the CKGM/HACC nanosphere encapsulation efficiency and in vitro controlled release properties.
27267571	2	45	from	complexation	412:423	arg1	solution					446:453	a neutral aqueous solution	428:453	a neutral aqueous solution without the use of chemical crosslinkers	428:494	In the present study, novel composite nanospheres were prepared from carboxymethyl konjac glucomannan (CKGM) and 2-hydroxypropyl trimethyl ammonium chloride chitosan (HACC) as a vaccine delivery vehicle by electrostatic complexation in a neutral aqueous solution without the use of chemical crosslinkers.
27267571	1	46	theme	useful	145:150	arg1	nanospheres					129:139	Biodegradable and biocompatible polymer nanospheres	89:139	Biodegradable and biocompatible polymer nanospheres	89:139	Biodegradable and biocompatible polymer nanospheres are useful materials for controlled drug delivery.
27267571	1	46	theme	useful	145:150	arg1	materials					152:160	useful materials	145:160	useful materials for controlled drug delivery	145:189	Biodegradable and biocompatible polymer nanospheres are useful materials for controlled drug delivery.
27267571	0	47	theme	konjac	48:53	arg1	glucomannan/chitosan					55:74	modified konjac glucomannan/chitosan	39:74	modified konjac glucomannan/chitosan	39:74	Preparation and sustainable release of modified konjac glucomannan/chitosan nanospheres.
27267571	4	48	theme	model	711:715	arg1	molecule					717:724	a model molecule	709:724	a model molecule for vaccines	709:737	Furthermore, using ovalbumin (OVA) as a model molecule for vaccines, various parameters were determined to affect the CKGM/HACC nanosphere encapsulation efficiency and in vitro controlled release properties.
27267571	4	48	theme	model	711:715	arg1	ovalbumin					690:698	ovalbumin	690:698	ovalbumin (OVA)	690:704	Furthermore, using ovalbumin (OVA) as a model molecule for vaccines, various parameters were determined to affect the CKGM/HACC nanosphere encapsulation efficiency and in vitro controlled release properties.
27267571	5	49	theme	OVA	1014:1016	arg1	release					1018:1024	sustained and continuous in vitro OVA release	980:1024	sustained and continuous in vitro OVA release over a period of more than 24h	980:1055	Under optimum conditions, the OVA encapsulation efficiency of CKGM/HACC nanospheres was 71.8%, while sustained and continuous in vitro OVA release over a period of more than 24h was observed.
27267571	4	50	theme	nanosphere	799:808	arg1	efficiency					824:833	the CKGM/HACC nanosphere encapsulation efficiency	785:833	the CKGM/HACC nanosphere encapsulation efficiency	785:833	Furthermore, using ovalbumin (OVA) as a model molecule for vaccines, various parameters were determined to affect the CKGM/HACC nanosphere encapsulation efficiency and in vitro controlled release properties.
27267571	3	51	theme	CKGM	513:516	arg1	concentrations					527:540	the CKGM and HACC concentrations	509:540	the CKGM and HACC concentrations	509:540	By altering the CKGM and HACC concentrations, the average CKGM/HACC particle size could be tuned from approximately 600nm to 1460nm and the zeta potential from 39mV to 50mV.
27267571	2	52	theme	composite	220:228	arg1	vehicle					387:393	a vaccine delivery vehicle	368:393	a vaccine delivery vehicle	368:393	In the present study, novel composite nanospheres were prepared from carboxymethyl konjac glucomannan (CKGM) and 2-hydroxypropyl trimethyl ammonium chloride chitosan (HACC) as a vaccine delivery vehicle by electrostatic complexation in a neutral aqueous solution without the use of chemical crosslinkers.
27267571	2	52	theme	composite	220:228	arg1	nanospheres					230:240	novel composite nanospheres	214:240	novel composite nanospheres	214:240	In the present study, novel composite nanospheres were prepared from carboxymethyl konjac glucomannan (CKGM) and 2-hydroxypropyl trimethyl ammonium chloride chitosan (HACC) as a vaccine delivery vehicle by electrostatic complexation in a neutral aqueous solution without the use of chemical crosslinkers.
27267571	4	53	theme	CKGM/HACC	789:797	arg1	efficiency					824:833	the CKGM/HACC nanosphere encapsulation efficiency	785:833	the CKGM/HACC nanosphere encapsulation efficiency	785:833	Furthermore, using ovalbumin (OVA) as a model molecule for vaccines, various parameters were determined to affect the CKGM/HACC nanosphere encapsulation efficiency and in vitro controlled release properties.
27267571	1	54	theme	controlled	166:175	arg1	delivery					182:189	controlled drug delivery	166:189	controlled drug delivery	166:189	Biodegradable and biocompatible polymer nanospheres are useful materials for controlled drug delivery.
27267571	2	55	theme	novel	214:218	arg1	vehicle					387:393	a vaccine delivery vehicle	368:393	a vaccine delivery vehicle	368:393	In the present study, novel composite nanospheres were prepared from carboxymethyl konjac glucomannan (CKGM) and 2-hydroxypropyl trimethyl ammonium chloride chitosan (HACC) as a vaccine delivery vehicle by electrostatic complexation in a neutral aqueous solution without the use of chemical crosslinkers.
27267571	2	55	theme	novel	214:218	arg1	nanospheres					230:240	novel composite nanospheres	214:240	novel composite nanospheres	214:240	In the present study, novel composite nanospheres were prepared from carboxymethyl konjac glucomannan (CKGM) and 2-hydroxypropyl trimethyl ammonium chloride chitosan (HACC) as a vaccine delivery vehicle by electrostatic complexation in a neutral aqueous solution without the use of chemical crosslinkers.
27267571	3	56	theme	zeta	637:640	arg1	potential					642:650	the zeta potential	633:650	the zeta potential from 39mV to 50mV	633:668	By altering the CKGM and HACC concentrations, the average CKGM/HACC particle size could be tuned from approximately 600nm to 1460nm and the zeta potential from 39mV to 50mV.
27267571	2	57	theme	electrostatic	398:410	arg1	complexation					412:423	electrostatic complexation	398:423	electrostatic complexation in a neutral aqueous solution without the use of chemical crosslinkers	398:494	In the present study, novel composite nanospheres were prepared from carboxymethyl konjac glucomannan (CKGM) and 2-hydroxypropyl trimethyl ammonium chloride chitosan (HACC) as a vaccine delivery vehicle by electrostatic complexation in a neutral aqueous solution without the use of chemical crosslinkers.
27267571	3	58	from	600nm	613:617	arg1	size					574:577	the average CKGM/HACC particle size	543:577	the average CKGM/HACC particle size	543:577	By altering the CKGM and HACC concentrations, the average CKGM/HACC particle size could be tuned from approximately 600nm to 1460nm and the zeta potential from 39mV to 50mV.
27267571	3	58	from	600nm	613:617	arg1	tuned					588:592	tuned	588:592	tuned	588:592	By altering the CKGM and HACC concentrations, the average CKGM/HACC particle size could be tuned from approximately 600nm to 1460nm and the zeta potential from 39mV to 50mV.
27267571	6	59	theme	delivery	1119:1126	arg1	carriers					1128:1135	novel drug delivery carriers	1108:1135	novel drug delivery carriers with great potential for medical applications	1108:1181	Therefore, CKGM/HACC nanospheres are novel drug delivery carriers with great potential for medical applications.
27267571	6	59	theme	delivery	1119:1126	arg1	nanospheres					1092:1102	CKGM/HACC nanospheres	1082:1102	CKGM/HACC nanospheres	1082:1102	Therefore, CKGM/HACC nanospheres are novel drug delivery carriers with great potential for medical applications.
27267571	4	60	theme	encapsulation	810:822	arg1	efficiency					824:833	the CKGM/HACC nanosphere encapsulation efficiency	785:833	the CKGM/HACC nanosphere encapsulation efficiency	785:833	Furthermore, using ovalbumin (OVA) as a model molecule for vaccines, various parameters were determined to affect the CKGM/HACC nanosphere encapsulation efficiency and in vitro controlled release properties.
27267571	3	61	theme	CKGM/HACC	555:563	arg1	size					574:577	the average CKGM/HACC particle size	543:577	the average CKGM/HACC particle size	543:577	By altering the CKGM and HACC concentrations, the average CKGM/HACC particle size could be tuned from approximately 600nm to 1460nm and the zeta potential from 39mV to 50mV.
27267571	3	61	theme	CKGM/HACC	555:563	arg1	tuned					588:592	tuned	588:592	tuned	588:592	By altering the CKGM and HACC concentrations, the average CKGM/HACC particle size could be tuned from approximately 600nm to 1460nm and the zeta potential from 39mV to 50mV.
26794770	3	0	theme	nanoparticle	540:551	arg1	ratio					583:587	the aspect ratio	572:587	the aspect ratio	572:587	Severe changes in the material morphology were observed by SEM and AFM due to a variation of the cellulose nanoparticle properties such as the aspect ratio, the crystalline index and the surface charge density.
26794770	3	0	theme	nanoparticle	540:551	arg1	properties					553:562	the cellulose nanoparticle properties	526:562	the cellulose nanoparticle properties such as the aspect ratio, the crystalline index and the surface charge density	526:641	Severe changes in the material morphology were observed by SEM and AFM due to a variation of the cellulose nanoparticle properties such as the aspect ratio, the crystalline index and the surface charge density.
26794770	3	0	theme	nanoparticle	540:551	arg1	density					635:641	the surface charge density	616:641	the surface charge density	616:641	Severe changes in the material morphology were observed by SEM and AFM due to a variation of the cellulose nanoparticle properties such as the aspect ratio, the crystalline index and the surface charge density.
26794770	3	0	theme	nanoparticle	540:551	arg1	index					606:610	the crystalline index	590:610	the crystalline index	590:610	Severe changes in the material morphology were observed by SEM and AFM due to a variation of the cellulose nanoparticle properties such as the aspect ratio, the crystalline index and the surface charge density.
26794770	1	1	theme	freeze-drying	296:308	arg1	technique					310:318	a freeze-drying technique	294:318	a freeze-drying technique	294:318	Bio-composite aerogels based on bleached cellulose fibers (BCF) and cellulose nanoparticles having various morphological and physico-chemical characteristics are prepared by a freeze-drying technique and characterized.
26794770	9	2	theme	mechanical	1550:1559	arg1	properties					1561:1570	the mechanical properties	1546:1570	the mechanical properties of the bio-composite aerogels	1546:1600	The characteristics of cellulose nanoparticles also influence the mechanical properties of the bio-composite aerogels.
26794770	7	3	theme	crystalline	1291:1301	arg1	index					1303:1307	a low crystalline index	1285:1307	a low crystalline index	1285:1307	This improvement of the insulation properties for composite materials is more pronounced for aerogels based on cellulose nanoparticles having a low crystalline index and high surface charge (NFC-2h).
26794770	11	4	theme	characteristics	1933:1947	arg1	morphology					1871:1880	the morphology	1867:1880	the morphology of the composite aerogel rather than the intrinsic characteristics of the cellulose nanoparticles	1867:1978	In this case, the mechanical property is more dependent on the morphology of the composite aerogel rather than the intrinsic characteristics of the cellulose nanoparticles.
26794770	1	5	theme	cellulose	188:196	arg1	nanoparticles					198:210	cellulose nanoparticles	188:210	cellulose nanoparticles having various morphological and physico-chemical characteristics	188:276	Bio-composite aerogels based on bleached cellulose fibers (BCF) and cellulose nanoparticles having various morphological and physico-chemical characteristics are prepared by a freeze-drying technique and characterized.
26794770	7	6	theme	high	1313:1316	arg1	NFC-2h					1334:1339	NFC-2h	1334:1339	NFC-2h	1334:1339	This improvement of the insulation properties for composite materials is more pronounced for aerogels based on cellulose nanoparticles having a low crystalline index and high surface charge (NFC-2h).
26794770	7	6	theme	high	1313:1316	arg1	charge					1326:1331	high surface charge	1313:1331	high surface charge (NFC-2h)	1313:1340	This improvement of the insulation properties for composite materials is more pronounced for aerogels based on cellulose nanoparticles having a low crystalline index and high surface charge (NFC-2h).
26794770	9	7	theme	bio-composite	1579:1591	arg1	aerogels					1593:1600	the bio-composite aerogels	1575:1600	the bio-composite aerogels	1575:1600	The characteristics of cellulose nanoparticles also influence the mechanical properties of the bio-composite aerogels.
26794770	2	8	theme	composite	351:359	arg1	aerogels					361:368	The various composite aerogels	339:368	The various composite aerogels obtained	339:377	The various composite aerogels obtained were compared to a BCF aerogel used as the reference.
26794770	5	9	theme	BET	841:843	arg1	analyses					845:852	BET analyses	841:852	BET analyses	841:852	BET analyses confirm the appearance of a new organization structure with pores of nanometric sizes.
26794770	3	10	theme	charge	628:633	arg1	density					635:641	the surface charge density	616:641	the surface charge density	616:641	Severe changes in the material morphology were observed by SEM and AFM due to a variation of the cellulose nanoparticle properties such as the aspect ratio, the crystalline index and the surface charge density.
26794770	1	11	theme	physico-chemical	245:260	arg1	characteristics					262:276	various morphological and physico-chemical characteristics	219:276	various morphological and physico-chemical characteristics	219:276	Bio-composite aerogels based on bleached cellulose fibers (BCF) and cellulose nanoparticles having various morphological and physico-chemical characteristics are prepared by a freeze-drying technique and characterized.
26794770	11	12	from	dependent	1854:1862	arg1	case					1816:1819	this case	1811:1819	this case	1811:1819	In this case, the mechanical property is more dependent on the morphology of the composite aerogel rather than the intrinsic characteristics of the cellulose nanoparticles.
26794770	10	13	theme	nanoparticles	1763:1775	arg1	architecture					1733:1744	a multi-scale architecture	1719:1744	a multi-scale architecture of the cellulose nanoparticles in the bio-composite aerogels	1719:1805	A significant improvement of the mechanical properties under compression is obtained by self-organization, yielding a multi-scale architecture of the cellulose nanoparticles in the bio-composite aerogels.
26794770	5	14	theme	sizes	934:938	arg1	pores					914:918	pores	914:918	pores of nanometric sizes	914:938	BET analyses confirm the appearance of a new organization structure with pores of nanometric sizes.
26794770	6	15	theme	28mWm	1017:1021	arg1	K					1026:1026	28mWm(-1)K(-1)	1017:1030	28mWm(-1)K(-1) (BCF aerogel) to 23mWm(-1)K(-1) (bio-composite aerogel)	1017:1086	As a consequence, a decrease of the thermal conductivities is observed from 28mWm(-1)K(-1) (BCF aerogel) to 23mWm(-1)K(-1) (bio-composite aerogel), which is below the air conductivity (25mWm(-1)K(-1)).
26794770	6	15	theme	28mWm	1017:1021	arg1	aerogel					1079:1085	bio-composite aerogel	1065:1085	bio-composite aerogel	1065:1085	As a consequence, a decrease of the thermal conductivities is observed from 28mWm(-1)K(-1) (BCF aerogel) to 23mWm(-1)K(-1) (bio-composite aerogel), which is below the air conductivity (25mWm(-1)K(-1)).
26794770	6	15	theme	28mWm	1017:1021	arg1	aerogel					1037:1043	BCF aerogel	1033:1043	BCF aerogel	1033:1043	As a consequence, a decrease of the thermal conductivities is observed from 28mWm(-1)K(-1) (BCF aerogel) to 23mWm(-1)K(-1) (bio-composite aerogel), which is below the air conductivity (25mWm(-1)K(-1)).
26794770	7	16	theme	composite	1193:1201	arg1	materials					1203:1211	composite materials	1193:1211	composite materials	1193:1211	This improvement of the insulation properties for composite materials is more pronounced for aerogels based on cellulose nanoparticles having a low crystalline index and high surface charge (NFC-2h).
26794770	6	17	dep	conductivity	1112:1123	arg1	-1					1137:1138	-1	1137:1138	-1	1137:1138	As a consequence, a decrease of the thermal conductivities is observed from 28mWm(-1)K(-1) (BCF aerogel) to 23mWm(-1)K(-1) (bio-composite aerogel), which is below the air conductivity (25mWm(-1)K(-1)).
26794770	6	17	dep	conductivity	1112:1123	arg1	K					1135:1135	25mWm(-1)K	1126:1135	25mWm(-1)K(-1)	1126:1139	As a consequence, a decrease of the thermal conductivities is observed from 28mWm(-1)K(-1) (BCF aerogel) to 23mWm(-1)K(-1) (bio-composite aerogel), which is below the air conductivity (25mWm(-1)K(-1)).
26794770	0	18	theme	super-insulating	68:83	arg1	properties					108:117	thermal super-insulating and tunable mechanical properties	60:117	thermal super-insulating and tunable mechanical properties	60:117	Multi-scale cellulose based new bio-aerogel composites with thermal super-insulating and tunable mechanical properties.
26794770	3	19	theme	aspect	576:581	arg1	ratio					583:587	the aspect ratio	572:587	the aspect ratio	572:587	Severe changes in the material morphology were observed by SEM and AFM due to a variation of the cellulose nanoparticle properties such as the aspect ratio, the crystalline index and the surface charge density.
26794770	4	20	from	comparison	799:808	arg1	size					778:781	the size	774:781	the size of the pores in comparison with a neat BCF based aerogel	774:838	BCF fibers form a 3D network and they are surrounded by the cellulose nanoparticle thin films inducing a significant reduction of the size of the pores in comparison with a neat BCF based aerogel.
26794770	5	21	theme	organization	886:897	arg1	structure					899:907	a new organization structure	880:907	a new organization structure	880:907	BET analyses confirm the appearance of a new organization structure with pores of nanometric sizes.
26794770	4	22	theme	nanoparticle	714:725	arg1	films					732:736	the cellulose nanoparticle thin films	700:736	the cellulose nanoparticle thin films inducing a significant reduction of the size of the pores in comparison with a neat BCF based aerogel	700:838	BCF fibers form a 3D network and they are surrounded by the cellulose nanoparticle thin films inducing a significant reduction of the size of the pores in comparison with a neat BCF based aerogel.
26794770	0	23	theme	tunable	89:95	arg1	properties					108:117	thermal super-insulating and tunable mechanical properties	60:117	thermal super-insulating and tunable mechanical properties	60:117	Multi-scale cellulose based new bio-aerogel composites with thermal super-insulating and tunable mechanical properties.
26794770	4	24	from	pores	790:794	arg1	comparison					799:808	comparison	799:808	comparison with a neat BCF based aerogel	799:838	BCF fibers form a 3D network and they are surrounded by the cellulose nanoparticle thin films inducing a significant reduction of the size of the pores in comparison with a neat BCF based aerogel.
26794770	6	25	theme	conductivities	985:998	arg1	consequence					946:956	a consequence	944:956	a consequence	944:956	As a consequence, a decrease of the thermal conductivities is observed from 28mWm(-1)K(-1) (BCF aerogel) to 23mWm(-1)K(-1) (bio-composite aerogel), which is below the air conductivity (25mWm(-1)K(-1)).
26794770	6	25	theme	conductivities	985:998	arg1	decrease					961:968	a decrease	959:968	a decrease of the thermal conductivities	959:998	As a consequence, a decrease of the thermal conductivities is observed from 28mWm(-1)K(-1) (BCF aerogel) to 23mWm(-1)K(-1) (bio-composite aerogel), which is below the air conductivity (25mWm(-1)K(-1)).
26794770	4	26	with	comparison	799:808	arg1	aerogel					832:838	a neat BCF based aerogel	815:838	a neat BCF based aerogel	815:838	BCF fibers form a 3D network and they are surrounded by the cellulose nanoparticle thin films inducing a significant reduction of the size of the pores in comparison with a neat BCF based aerogel.
26794770	1	27	theme	cellulose	161:169	arg1	BCF					179:181	BCF	179:181	BCF	179:181	Bio-composite aerogels based on bleached cellulose fibers (BCF) and cellulose nanoparticles having various morphological and physico-chemical characteristics are prepared by a freeze-drying technique and characterized.
26794770	1	27	theme	cellulose	161:169	arg1	fibers					171:176	bleached cellulose fibers	152:176	bleached cellulose fibers (BCF)	152:182	Bio-composite aerogels based on bleached cellulose fibers (BCF) and cellulose nanoparticles having various morphological and physico-chemical characteristics are prepared by a freeze-drying technique and characterized.
26794770	4	28	theme	pores	790:794	arg1	size					778:781	the size	774:781	the size of the pores in comparison with a neat BCF based aerogel	774:838	BCF fibers form a 3D network and they are surrounded by the cellulose nanoparticle thin films inducing a significant reduction of the size of the pores in comparison with a neat BCF based aerogel.
26794770	3	29	theme	Severe	433:438	arg1	changes					440:446	Severe changes	433:446	Severe changes in the material morphology	433:473	Severe changes in the material morphology were observed by SEM and AFM due to a variation of the cellulose nanoparticle properties such as the aspect ratio, the crystalline index and the surface charge density.
26794770	8	30	theme	properties	1391:1400	arg1	improvement					1359:1369	The significant improvement	1343:1369	The significant improvement of their insulation properties	1343:1400	The significant improvement of their insulation properties allows the bio-composite aerogels to enter the super-insulating materials family.
26794770	0	31	theme	Multi-scale	0:10	arg1	cellulose					12:20	Multi-scale cellulose	0:20	Multi-scale cellulose	0:20	Multi-scale cellulose based new bio-aerogel composites with thermal super-insulating and tunable mechanical properties.
26794770	7	32	theme	insulation	1167:1176	arg1	properties					1178:1187	the insulation properties	1163:1187	the insulation properties for composite materials	1163:1211	This improvement of the insulation properties for composite materials is more pronounced for aerogels based on cellulose nanoparticles having a low crystalline index and high surface charge (NFC-2h).
26794770	11	33	theme	aerogel	1899:1905	arg1	morphology					1871:1880	the morphology	1867:1880	the morphology of the composite aerogel rather than the intrinsic characteristics of the cellulose nanoparticles	1867:1978	In this case, the mechanical property is more dependent on the morphology of the composite aerogel rather than the intrinsic characteristics of the cellulose nanoparticles.
26794770	3	34	theme	due	504:506	arg1	SEM					492:494	SEM	492:494	SEM	492:494	Severe changes in the material morphology were observed by SEM and AFM due to a variation of the cellulose nanoparticle properties such as the aspect ratio, the crystalline index and the surface charge density.
26794770	7	35	contain	having	1278:1283	arg1	nanoparticles					1264:1276	cellulose nanoparticles	1254:1276	cellulose nanoparticles having a low crystalline index and high surface charge (NFC-2h)	1254:1340	This improvement of the insulation properties for composite materials is more pronounced for aerogels based on cellulose nanoparticles having a low crystalline index and high surface charge (NFC-2h).
26794770	7	35	contain	having	1278:1283	arg2	index					1303:1307	a low crystalline index	1285:1307	a low crystalline index	1285:1307	This improvement of the insulation properties for composite materials is more pronounced for aerogels based on cellulose nanoparticles having a low crystalline index and high surface charge (NFC-2h).
26794770	7	35	contain	having	1278:1283	arg2	NFC-2h					1334:1339	NFC-2h	1334:1339	NFC-2h	1334:1339	This improvement of the insulation properties for composite materials is more pronounced for aerogels based on cellulose nanoparticles having a low crystalline index and high surface charge (NFC-2h).
26794770	7	35	contain	having	1278:1283	arg2	charge					1326:1331	high surface charge	1313:1331	high surface charge (NFC-2h)	1313:1340	This improvement of the insulation properties for composite materials is more pronounced for aerogels based on cellulose nanoparticles having a low crystalline index and high surface charge (NFC-2h).
26794770	3	36	theme	material	455:462	arg1	morphology					464:473	the material morphology	451:473	the material morphology	451:473	Severe changes in the material morphology were observed by SEM and AFM due to a variation of the cellulose nanoparticle properties such as the aspect ratio, the crystalline index and the surface charge density.
26794770	4	37	theme	BCF	822:824	arg1	aerogel					832:838	a neat BCF based aerogel	815:838	a neat BCF based aerogel	815:838	BCF fibers form a 3D network and they are surrounded by the cellulose nanoparticle thin films inducing a significant reduction of the size of the pores in comparison with a neat BCF based aerogel.
26794770	0	38	theme	bio-aerogel	32:42	arg1	composites					44:53	new bio-aerogel composites	28:53	new bio-aerogel composites	28:53	Multi-scale cellulose based new bio-aerogel composites with thermal super-insulating and tunable mechanical properties.
26794770	2	39	theme	BCF	398:400	arg1	aerogel					402:408	a BCF aerogel	396:408	a BCF aerogel used as the reference	396:430	The various composite aerogels obtained were compared to a BCF aerogel used as the reference.
26794770	8	40	theme	super-insulating	1449:1464	arg1	family					1476:1481	the super-insulating materials family	1445:1481	the super-insulating materials family	1445:1481	The significant improvement of their insulation properties allows the bio-composite aerogels to enter the super-insulating materials family.
26794770	6	41	theme	bio-composite	1065:1077	arg1	K					1026:1026	28mWm(-1)K(-1)	1017:1030	28mWm(-1)K(-1) (BCF aerogel) to 23mWm(-1)K(-1) (bio-composite aerogel)	1017:1086	As a consequence, a decrease of the thermal conductivities is observed from 28mWm(-1)K(-1) (BCF aerogel) to 23mWm(-1)K(-1) (bio-composite aerogel), which is below the air conductivity (25mWm(-1)K(-1)).
26794770	6	41	theme	bio-composite	1065:1077	arg1	aerogel					1079:1085	bio-composite aerogel	1065:1085	bio-composite aerogel	1065:1085	As a consequence, a decrease of the thermal conductivities is observed from 28mWm(-1)K(-1) (BCF aerogel) to 23mWm(-1)K(-1) (bio-composite aerogel), which is below the air conductivity (25mWm(-1)K(-1)).
26794770	9	42	theme	cellulose	1507:1515	arg1	nanoparticles					1517:1529	cellulose nanoparticles	1507:1529	cellulose nanoparticles	1507:1529	The characteristics of cellulose nanoparticles also influence the mechanical properties of the bio-composite aerogels.
26794770	10	43	theme	properties	1647:1656	arg1	improvement					1617:1627	A significant improvement	1603:1627	A significant improvement of the mechanical properties under compression	1603:1674	A significant improvement of the mechanical properties under compression is obtained by self-organization, yielding a multi-scale architecture of the cellulose nanoparticles in the bio-composite aerogels.
26794770	4	44	theme	BCF	644:646	arg1	fibers					648:653	BCF fibers	644:653	BCF fibers	644:653	BCF fibers form a 3D network and they are surrounded by the cellulose nanoparticle thin films inducing a significant reduction of the size of the pores in comparison with a neat BCF based aerogel.
26794770	3	45	theme	cellulose	530:538	arg1	ratio					583:587	the aspect ratio	572:587	the aspect ratio	572:587	Severe changes in the material morphology were observed by SEM and AFM due to a variation of the cellulose nanoparticle properties such as the aspect ratio, the crystalline index and the surface charge density.
26794770	3	45	theme	cellulose	530:538	arg1	properties					553:562	the cellulose nanoparticle properties	526:562	the cellulose nanoparticle properties such as the aspect ratio, the crystalline index and the surface charge density	526:641	Severe changes in the material morphology were observed by SEM and AFM due to a variation of the cellulose nanoparticle properties such as the aspect ratio, the crystalline index and the surface charge density.
26794770	3	45	theme	cellulose	530:538	arg1	density					635:641	the surface charge density	616:641	the surface charge density	616:641	Severe changes in the material morphology were observed by SEM and AFM due to a variation of the cellulose nanoparticle properties such as the aspect ratio, the crystalline index and the surface charge density.
26794770	3	45	theme	cellulose	530:538	arg1	index					606:610	the crystalline index	590:610	the crystalline index	590:610	Severe changes in the material morphology were observed by SEM and AFM due to a variation of the cellulose nanoparticle properties such as the aspect ratio, the crystalline index and the surface charge density.
26794770	10	46	from	aerogels	1798:1805	arg1	architecture					1733:1744	a multi-scale architecture	1719:1744	a multi-scale architecture of the cellulose nanoparticles in the bio-composite aerogels	1719:1805	A significant improvement of the mechanical properties under compression is obtained by self-organization, yielding a multi-scale architecture of the cellulose nanoparticles in the bio-composite aerogels.
26794770	1	47	theme	morphological	227:239	arg1	characteristics					262:276	various morphological and physico-chemical characteristics	219:276	various morphological and physico-chemical characteristics	219:276	Bio-composite aerogels based on bleached cellulose fibers (BCF) and cellulose nanoparticles having various morphological and physico-chemical characteristics are prepared by a freeze-drying technique and characterized.
26794770	2	48	theme	various	343:349	arg1	aerogels					361:368	The various composite aerogels	339:368	The various composite aerogels obtained	339:377	The various composite aerogels obtained were compared to a BCF aerogel used as the reference.
26794770	7	49	theme	surface	1318:1324	arg1	NFC-2h					1334:1339	NFC-2h	1334:1339	NFC-2h	1334:1339	This improvement of the insulation properties for composite materials is more pronounced for aerogels based on cellulose nanoparticles having a low crystalline index and high surface charge (NFC-2h).
26794770	7	49	theme	surface	1318:1324	arg1	charge					1326:1331	high surface charge	1313:1331	high surface charge (NFC-2h)	1313:1340	This improvement of the insulation properties for composite materials is more pronounced for aerogels based on cellulose nanoparticles having a low crystalline index and high surface charge (NFC-2h).
26794770	11	50	theme	intrinsic	1923:1931	arg1	characteristics					1933:1947	the intrinsic characteristics	1919:1947	the composite aerogel rather than the intrinsic characteristics of the cellulose nanoparticles	1885:1978	In this case, the mechanical property is more dependent on the morphology of the composite aerogel rather than the intrinsic characteristics of the cellulose nanoparticles.
26794770	7	51	theme	low	1287:1289	arg1	index					1303:1307	a low crystalline index	1285:1307	a low crystalline index	1285:1307	This improvement of the insulation properties for composite materials is more pronounced for aerogels based on cellulose nanoparticles having a low crystalline index and high surface charge (NFC-2h).
26794770	3	52	theme	crystalline	594:604	arg1	index					606:610	the crystalline index	590:610	the crystalline index	590:610	Severe changes in the material morphology were observed by SEM and AFM due to a variation of the cellulose nanoparticle properties such as the aspect ratio, the crystalline index and the surface charge density.
26794770	9	53	theme	aerogels	1593:1600	arg1	properties					1561:1570	the mechanical properties	1546:1570	the mechanical properties of the bio-composite aerogels	1546:1600	The characteristics of cellulose nanoparticles also influence the mechanical properties of the bio-composite aerogels.
26794770	11	54	theme	cellulose	1956:1964	arg1	nanoparticles					1966:1978	the cellulose nanoparticles	1952:1978	the cellulose nanoparticles	1952:1978	In this case, the mechanical property is more dependent on the morphology of the composite aerogel rather than the intrinsic characteristics of the cellulose nanoparticles.
26794770	1	55	theme	various	219:225	arg1	characteristics					262:276	various morphological and physico-chemical characteristics	219:276	various morphological and physico-chemical characteristics	219:276	Bio-composite aerogels based on bleached cellulose fibers (BCF) and cellulose nanoparticles having various morphological and physico-chemical characteristics are prepared by a freeze-drying technique and characterized.
26794770	7	56	theme	cellulose	1254:1262	arg1	nanoparticles					1264:1276	cellulose nanoparticles	1254:1276	cellulose nanoparticles having a low crystalline index and high surface charge (NFC-2h)	1254:1340	This improvement of the insulation properties for composite materials is more pronounced for aerogels based on cellulose nanoparticles having a low crystalline index and high surface charge (NFC-2h).
26794770	3	57	theme	surface	620:626	arg1	density					635:641	the surface charge density	616:641	the surface charge density	616:641	Severe changes in the material morphology were observed by SEM and AFM due to a variation of the cellulose nanoparticle properties such as the aspect ratio, the crystalline index and the surface charge density.
26794770	4	58	theme	3D	662:663	arg1	network					665:671	a 3D network	660:671	a 3D network	660:671	BCF fibers form a 3D network and they are surrounded by the cellulose nanoparticle thin films inducing a significant reduction of the size of the pores in comparison with a neat BCF based aerogel.
26794770	10	59	theme	multi-scale	1721:1731	arg1	architecture					1733:1744	a multi-scale architecture	1719:1744	a multi-scale architecture of the cellulose nanoparticles in the bio-composite aerogels	1719:1805	A significant improvement of the mechanical properties under compression is obtained by self-organization, yielding a multi-scale architecture of the cellulose nanoparticles in the bio-composite aerogels.
26794770	6	60	theme	air	1108:1110	arg1	conductivity					1112:1123	the air conductivity	1104:1123	the air conductivity (25mWm(-1)K(-1))	1104:1140	As a consequence, a decrease of the thermal conductivities is observed from 28mWm(-1)K(-1) (BCF aerogel) to 23mWm(-1)K(-1) (bio-composite aerogel), which is below the air conductivity (25mWm(-1)K(-1)).
26794770	4	61	theme	thin	727:730	arg1	films					732:736	the cellulose nanoparticle thin films	700:736	the cellulose nanoparticle thin films inducing a significant reduction of the size of the pores in comparison with a neat BCF based aerogel	700:838	BCF fibers form a 3D network and they are surrounded by the cellulose nanoparticle thin films inducing a significant reduction of the size of the pores in comparison with a neat BCF based aerogel.
26794770	1	62	theme	bleached	152:159	arg1	BCF					179:181	BCF	179:181	BCF	179:181	Bio-composite aerogels based on bleached cellulose fibers (BCF) and cellulose nanoparticles having various morphological and physico-chemical characteristics are prepared by a freeze-drying technique and characterized.
26794770	1	62	theme	bleached	152:159	arg1	fibers					171:176	bleached cellulose fibers	152:176	bleached cellulose fibers (BCF)	152:182	Bio-composite aerogels based on bleached cellulose fibers (BCF) and cellulose nanoparticles having various morphological and physico-chemical characteristics are prepared by a freeze-drying technique and characterized.
26794770	10	63	theme	cellulose	1753:1761	arg1	nanoparticles					1763:1775	the cellulose nanoparticles	1749:1775	the cellulose nanoparticles in the bio-composite aerogels	1749:1805	A significant improvement of the mechanical properties under compression is obtained by self-organization, yielding a multi-scale architecture of the cellulose nanoparticles in the bio-composite aerogels.
26794770	5	64	theme	new	882:884	arg1	structure					899:907	a new organization structure	880:907	a new organization structure	880:907	BET analyses confirm the appearance of a new organization structure with pores of nanometric sizes.
26794770	8	65	theme	significant	1347:1357	arg1	improvement					1359:1369	The significant improvement	1343:1369	The significant improvement of their insulation properties	1343:1400	The significant improvement of their insulation properties allows the bio-composite aerogels to enter the super-insulating materials family.
26794770	0	66	theme	mechanical	97:106	arg1	properties					108:117	thermal super-insulating and tunable mechanical properties	60:117	thermal super-insulating and tunable mechanical properties	60:117	Multi-scale cellulose based new bio-aerogel composites with thermal super-insulating and tunable mechanical properties.
26794770	5	67	theme	structure	899:907	arg1	appearance					866:875	the appearance	862:875	the appearance of a new organization structure with pores of nanometric sizes	862:938	BET analyses confirm the appearance of a new organization structure with pores of nanometric sizes.
26794770	4	68	theme	cellulose	704:712	arg1	films					732:736	the cellulose nanoparticle thin films	700:736	the cellulose nanoparticle thin films inducing a significant reduction of the size of the pores in comparison with a neat BCF based aerogel	700:838	BCF fibers form a 3D network and they are surrounded by the cellulose nanoparticle thin films inducing a significant reduction of the size of the pores in comparison with a neat BCF based aerogel.
26794770	11	69	theme	nanoparticles	1966:1978	arg1	characteristics					1933:1947	the intrinsic characteristics	1919:1947	the composite aerogel rather than the intrinsic characteristics of the cellulose nanoparticles	1885:1978	In this case, the mechanical property is more dependent on the morphology of the composite aerogel rather than the intrinsic characteristics of the cellulose nanoparticles.
26794770	6	70	theme	thermal	977:983	arg1	conductivities					985:998	the thermal conductivities	973:998	the thermal conductivities	973:998	As a consequence, a decrease of the thermal conductivities is observed from 28mWm(-1)K(-1) (BCF aerogel) to 23mWm(-1)K(-1) (bio-composite aerogel), which is below the air conductivity (25mWm(-1)K(-1)).
26794770	8	71	theme	insulation	1380:1389	arg1	properties					1391:1400	their insulation properties	1374:1400	their insulation properties	1374:1400	The significant improvement of their insulation properties allows the bio-composite aerogels to enter the super-insulating materials family.
26794770	11	72	from	case	1816:1819	arg1	property					1837:1844	the mechanical property	1822:1844	the mechanical property	1822:1844	In this case, the mechanical property is more dependent on the morphology of the composite aerogel rather than the intrinsic characteristics of the cellulose nanoparticles.
26794770	11	72	from	case	1816:1819	arg1	dependent					1854:1862	dependent	1854:1862	dependent	1854:1862	In this case, the mechanical property is more dependent on the morphology of the composite aerogel rather than the intrinsic characteristics of the cellulose nanoparticles.
26794770	4	73	theme	size	778:781	arg1	reduction					761:769	a significant reduction	747:769	a significant reduction of the size of the pores in comparison with a neat BCF based aerogel	747:838	BCF fibers form a 3D network and they are surrounded by the cellulose nanoparticle thin films inducing a significant reduction of the size of the pores in comparison with a neat BCF based aerogel.
26794770	4	74	from	size	778:781	arg1	comparison					799:808	comparison	799:808	comparison with a neat BCF based aerogel	799:838	BCF fibers form a 3D network and they are surrounded by the cellulose nanoparticle thin films inducing a significant reduction of the size of the pores in comparison with a neat BCF based aerogel.
26794770	5	75	theme	nanometric	923:932	arg1	sizes					934:938	nanometric sizes	923:938	nanometric sizes	923:938	BET analyses confirm the appearance of a new organization structure with pores of nanometric sizes.
26794770	7	76	theme	properties	1178:1187	arg1	improvement					1148:1158	This improvement	1143:1158	This improvement of the insulation properties for composite materials	1143:1211	This improvement of the insulation properties for composite materials is more pronounced for aerogels based on cellulose nanoparticles having a low crystalline index and high surface charge (NFC-2h).
26794770	6	77	dep	K	1026:1026	arg1	to					1046:1047	to	1046:1047	to	1046:1047	As a consequence, a decrease of the thermal conductivities is observed from 28mWm(-1)K(-1) (BCF aerogel) to 23mWm(-1)K(-1) (bio-composite aerogel), which is below the air conductivity (25mWm(-1)K(-1)).
26794770	6	77	dep	K	1026:1026	arg1	K					1058:1058	23mWm(-1)K(-1)	1049:1062	28mWm(-1)K(-1) (BCF aerogel) to 23mWm(-1)K(-1) (bio-composite aerogel)	1017:1086	As a consequence, a decrease of the thermal conductivities is observed from 28mWm(-1)K(-1) (BCF aerogel) to 23mWm(-1)K(-1) (bio-composite aerogel), which is below the air conductivity (25mWm(-1)K(-1)).
26794770	3	78	from	changes	440:446	arg1	morphology					464:473	the material morphology	451:473	the material morphology	451:473	Severe changes in the material morphology were observed by SEM and AFM due to a variation of the cellulose nanoparticle properties such as the aspect ratio, the crystalline index and the surface charge density.
26794770	10	79	from	architecture	1733:1744	arg1	aerogels					1798:1805	the bio-composite aerogels	1780:1805	the bio-composite aerogels	1780:1805	A significant improvement of the mechanical properties under compression is obtained by self-organization, yielding a multi-scale architecture of the cellulose nanoparticles in the bio-composite aerogels.
26794770	8	80	theme	bio-composite	1413:1425	arg1	aerogels					1427:1434	the bio-composite aerogels	1409:1434	the bio-composite aerogels	1409:1434	The significant improvement of their insulation properties allows the bio-composite aerogels to enter the super-insulating materials family.
26794770	4	81	theme	significant	749:759	arg1	reduction					761:769	a significant reduction	747:769	a significant reduction of the size of the pores in comparison with a neat BCF based aerogel	747:838	BCF fibers form a 3D network and they are surrounded by the cellulose nanoparticle thin films inducing a significant reduction of the size of the pores in comparison with a neat BCF based aerogel.
26794770	11	82	theme	mechanical	1826:1835	arg1	property					1837:1844	the mechanical property	1822:1844	the mechanical property	1822:1844	In this case, the mechanical property is more dependent on the morphology of the composite aerogel rather than the intrinsic characteristics of the cellulose nanoparticles.
26794770	11	82	theme	mechanical	1826:1835	arg1	dependent					1854:1862	dependent	1854:1862	dependent	1854:1862	In this case, the mechanical property is more dependent on the morphology of the composite aerogel rather than the intrinsic characteristics of the cellulose nanoparticles.
26794770	0	83	theme	new	28:30	arg1	composites					44:53	new bio-aerogel composites	28:53	new bio-aerogel composites	28:53	Multi-scale cellulose based new bio-aerogel composites with thermal super-insulating and tunable mechanical properties.
26794770	1	84	theme	Bio-composite	120:132	arg1	aerogels					134:141	Bio-composite aerogels	120:141	Bio-composite aerogels based on bleached cellulose fibers (BCF) and cellulose nanoparticles having various morphological and physico-chemical characteristics	120:276	Bio-composite aerogels based on bleached cellulose fibers (BCF) and cellulose nanoparticles having various morphological and physico-chemical characteristics are prepared by a freeze-drying technique and characterized.
26794770	1	85	contain	having	212:217	arg1	nanoparticles					198:210	cellulose nanoparticles	188:210	cellulose nanoparticles having various morphological and physico-chemical characteristics	188:276	Bio-composite aerogels based on bleached cellulose fibers (BCF) and cellulose nanoparticles having various morphological and physico-chemical characteristics are prepared by a freeze-drying technique and characterized.
26794770	1	85	contain	having	212:217	arg2	characteristics					262:276	various morphological and physico-chemical characteristics	219:276	various morphological and physico-chemical characteristics	219:276	Bio-composite aerogels based on bleached cellulose fibers (BCF) and cellulose nanoparticles having various morphological and physico-chemical characteristics are prepared by a freeze-drying technique and characterized.
26794770	3	86	theme	properties	553:562	arg1	variation					513:521	a variation	511:521	a variation of the cellulose nanoparticle properties such as the aspect ratio, the crystalline index and the surface charge density	511:641	Severe changes in the material morphology were observed by SEM and AFM due to a variation of the cellulose nanoparticle properties such as the aspect ratio, the crystalline index and the surface charge density.
26794770	10	87	from	nanoparticles	1763:1775	arg1	aerogels					1798:1805	the bio-composite aerogels	1780:1805	the bio-composite aerogels	1780:1805	A significant improvement of the mechanical properties under compression is obtained by self-organization, yielding a multi-scale architecture of the cellulose nanoparticles in the bio-composite aerogels.
26794770	6	88	theme	BCF	1033:1035	arg1	K					1026:1026	28mWm(-1)K(-1)	1017:1030	28mWm(-1)K(-1) (BCF aerogel) to 23mWm(-1)K(-1) (bio-composite aerogel)	1017:1086	As a consequence, a decrease of the thermal conductivities is observed from 28mWm(-1)K(-1) (BCF aerogel) to 23mWm(-1)K(-1) (bio-composite aerogel), which is below the air conductivity (25mWm(-1)K(-1)).
26794770	6	88	theme	BCF	1033:1035	arg1	aerogel					1037:1043	BCF aerogel	1033:1043	BCF aerogel	1033:1043	As a consequence, a decrease of the thermal conductivities is observed from 28mWm(-1)K(-1) (BCF aerogel) to 23mWm(-1)K(-1) (bio-composite aerogel), which is below the air conductivity (25mWm(-1)K(-1)).
26794770	10	89	theme	bio-composite	1784:1796	arg1	aerogels					1798:1805	the bio-composite aerogels	1780:1805	the bio-composite aerogels	1780:1805	A significant improvement of the mechanical properties under compression is obtained by self-organization, yielding a multi-scale architecture of the cellulose nanoparticles in the bio-composite aerogels.
26794770	4	90	theme	neat	817:820	arg1	aerogel					832:838	a neat BCF based aerogel	815:838	a neat BCF based aerogel	815:838	BCF fibers form a 3D network and they are surrounded by the cellulose nanoparticle thin films inducing a significant reduction of the size of the pores in comparison with a neat BCF based aerogel.
26794770	0	91	theme	thermal	60:66	arg1	properties					108:117	thermal super-insulating and tunable mechanical properties	60:117	thermal super-insulating and tunable mechanical properties	60:117	Multi-scale cellulose based new bio-aerogel composites with thermal super-insulating and tunable mechanical properties.
26794770	8	92	theme	materials	1466:1474	arg1	family					1476:1481	the super-insulating materials family	1445:1481	the super-insulating materials family	1445:1481	The significant improvement of their insulation properties allows the bio-composite aerogels to enter the super-insulating materials family.
26794770	4	93	theme	based	826:830	arg1	aerogel					832:838	a neat BCF based aerogel	815:838	a neat BCF based aerogel	815:838	BCF fibers form a 3D network and they are surrounded by the cellulose nanoparticle thin films inducing a significant reduction of the size of the pores in comparison with a neat BCF based aerogel.
26794770	10	94	theme	significant	1605:1615	arg1	improvement					1617:1627	A significant improvement	1603:1627	A significant improvement of the mechanical properties under compression	1603:1674	A significant improvement of the mechanical properties under compression is obtained by self-organization, yielding a multi-scale architecture of the cellulose nanoparticles in the bio-composite aerogels.
26794770	11	95	theme	composite	1889:1897	arg1	aerogel					1899:1905	the composite aerogel	1885:1905	the composite aerogel rather than the intrinsic characteristics of the cellulose nanoparticles	1885:1978	In this case, the mechanical property is more dependent on the morphology of the composite aerogel rather than the intrinsic characteristics of the cellulose nanoparticles.
26794770	5	96	with	appearance	866:875	arg1	pores					914:918	pores	914:918	pores of nanometric sizes	914:938	BET analyses confirm the appearance of a new organization structure with pores of nanometric sizes.
26794770	9	97	theme	nanoparticles	1517:1529	arg1	characteristics					1488:1502	The characteristics	1484:1502	The characteristics of cellulose nanoparticles	1484:1529	The characteristics of cellulose nanoparticles also influence the mechanical properties of the bio-composite aerogels.
26794770	6	98	theme	23mWm	1049:1053	arg1	K					1058:1058	23mWm(-1)K(-1)	1049:1062	28mWm(-1)K(-1) (BCF aerogel) to 23mWm(-1)K(-1) (bio-composite aerogel)	1017:1086	As a consequence, a decrease of the thermal conductivities is observed from 28mWm(-1)K(-1) (BCF aerogel) to 23mWm(-1)K(-1) (bio-composite aerogel), which is below the air conductivity (25mWm(-1)K(-1)).
26794770	10	99	theme	mechanical	1636:1645	arg1	properties					1647:1656	the mechanical properties	1632:1656	the mechanical properties under compression	1632:1674	A significant improvement of the mechanical properties under compression is obtained by self-organization, yielding a multi-scale architecture of the cellulose nanoparticles in the bio-composite aerogels.
25615402	7	0	theme	Grain	1068:1072	arg1	starch					1074:1079	(ii) Grain starch	1063:1079	(ii) Grain starch	1063:1079	(ii) Grain starch increased in Seedling EC4 (32.6%) and Anthesis EC2 (39%), respectively, suggesting a stimulatory effect of salt on starch accumulation.
25615402	1	1	from	compositional	175:187	arg1	rice					216:219	rice	216:219	rice	216:219	The aim of this work was to examine agronomic, compositional, and functional changes in rice (Oryza sativa L. cv.
25615402	8	2	theme	grain	1350:1354	arg1	weight					1356:1361	reduced grain weight	1342:1361	reduced grain weight (28.8%)	1342:1369	(iii) The salinity treatment of 2 dS/m(2) was better tolerated at anthesis than the 4 dS/m(2) treatment as the latter led to reduced grain weight (28.8%) and seed fertility (19.4%) and compensatory increases in protein (20.1%) and nitrogen (19.8%) contents.
25615402	8	2	theme	grain	1350:1354	arg1	%					1368:1368	28.8%	1364:1368	28.8%	1364:1368	(iii) The salinity treatment of 2 dS/m(2) was better tolerated at anthesis than the 4 dS/m(2) treatment as the latter led to reduced grain weight (28.8%) and seed fertility (19.4%) and compensatory increases in protein (20.1%) and nitrogen (19.8%) contents.
25615402	13	3	theme	defined	2169:2175	arg1	conditions					2177:2186	defined conditions	2169:2186	defined conditions	2169:2186	Overall, this study documents the specific response of rice under defined conditions, and illustrates that the plasticity of plant response to mild stress is complex and highly context-dependent, even under greenhouse conditions in which other potential environmental stress impacts are minimized.
25615402	13	4	theme	stress	2371:2376	arg1	impacts					2378:2384	other potential environmental stress impacts	2341:2384	other potential environmental stress impacts	2341:2384	Overall, this study documents the specific response of rice under defined conditions, and illustrates that the plasticity of plant response to mild stress is complex and highly context-dependent, even under greenhouse conditions in which other potential environmental stress impacts are minimized.
25615402	1	5	theme	functional	194:203	arg1	changes					205:211	functional changes	194:211	functional changes in rice	194:219	The aim of this work was to examine agronomic, compositional, and functional changes in rice (Oryza sativa L. cv.
25615402	13	6	theme	environmental	2357:2369	arg1	impacts					2378:2384	other potential environmental stress impacts	2341:2384	other potential environmental stress impacts	2341:2384	Overall, this study documents the specific response of rice under defined conditions, and illustrates that the plasticity of plant response to mild stress is complex and highly context-dependent, even under greenhouse conditions in which other potential environmental stress impacts are minimized.
25615402	6	7	theme	control	1011:1017	arg1	group					1019:1023	the control group	1007:1023	the control group	1007:1023	Seedling EC2 plants had more panicles and enhanced caryopsis dimensions, while surprisingly, Seedling EC4 plants did not differ from the control group in the agronomic parameters measured.
25615402	11	8	theme	Anthesis	1891:1898	arg1	treatment					1904:1912	the Anthesis EC4 treatment	1887:1912	the Anthesis EC4 treatment	1887:1912	The only alterations in starch were limited to small changes in thermal properties and glucan chain distribution, which were only seen in the Anthesis EC4 treatment.
25615402	12	9	theme	multivariate	1989:2000	arg1	analysis					2002:2009	multivariate analysis	1989:2009	multivariate analysis	1989:2009	This similarity of compositional and functional features was supported by multivariate analysis of all variables measured, which suggested that differences due to treatments were minimal.
25615402	8	10	theme	compensatory	1402:1413	arg1	increases					1415:1423	compensatory increases	1402:1423	compensatory increases in protein (20.1%) and nitrogen (19.8%) contents	1402:1472	(iii) The salinity treatment of 2 dS/m(2) was better tolerated at anthesis than the 4 dS/m(2) treatment as the latter led to reduced grain weight (28.8%) and seed fertility (19.4%) and compensatory increases in protein (20.1%) and nitrogen (19.8%) contents.
25615402	5	11	theme	study	729:733	arg1	findings					712:719	Key findings	708:719	Key findings of this study	708:733	Key findings of this study are the following: (i) Plants showed adaptive responses to prolonged salt treatment with no negative effects on grain weight or fertility.
25615402	5	11	theme	study	729:733	arg1	following					743:751	the following	739:751	the following: (i) Plants showed adaptive responses to prolonged salt treatment with no negative effects on grain weight or fertility	739:871	Key findings of this study are the following: (i) Plants showed adaptive responses to prolonged salt treatment with no negative effects on grain weight or fertility.
25615402	11	12	theme	only	1753:1756	arg1	alterations					1758:1768	The only alterations	1749:1768	The only alterations in starch	1749:1778	The only alterations in starch were limited to small changes in thermal properties and glucan chain distribution, which were only seen in the Anthesis EC4 treatment.
25615402	11	12	theme	only	1753:1756	arg1	limited					1785:1791	limited	1785:1791	limited	1785:1791	The only alterations in starch were limited to small changes in thermal properties and glucan chain distribution, which were only seen in the Anthesis EC4 treatment.
25615402	13	13	theme	rice	2158:2161	arg1	response					2146:2153	the specific response	2133:2153	the specific response of rice	2133:2161	Overall, this study documents the specific response of rice under defined conditions, and illustrates that the plasticity of plant response to mild stress is complex and highly context-dependent, even under greenhouse conditions in which other potential environmental stress impacts are minimized.
25615402	3	14	theme	called	475:480	arg1	EC2					491:493	Seedling EC2	482:493	Seedling EC2	482:493	Plants were grown in sodium chloride-containing soil (2 or 4 dS/m(2) electrical conductivity), which was imposed 4-weeks after transplant (called Seedling EC2 and EC4) or after the appearance of the anthers (called Anthesis EC2 and EC4).
25615402	9	15	theme	starch	1568:1573	arg1	structure					1580:1588	starch fine structure	1568:1588	starch fine structure	1568:1588	(iv) Although some salinity treatments led to changes in starch content, these did not alter starch fine structure, morphology, or composition.
25615402	5	16	theme	Key	708:710	arg1	findings					712:719	Key findings	708:719	Key findings of this study	708:733	Key findings of this study are the following: (i) Plants showed adaptive responses to prolonged salt treatment with no negative effects on grain weight or fertility.
25615402	5	16	theme	Key	708:710	arg1	following					743:751	the following	739:751	the following: (i) Plants showed adaptive responses to prolonged salt treatment with no negative effects on grain weight or fertility	739:871	Key findings of this study are the following: (i) Plants showed adaptive responses to prolonged salt treatment with no negative effects on grain weight or fertility.
25615402	13	17	dep	complex	2261:2267	arg1	complex					2261:2267	complex	2261:2267	complex	2261:2267	Overall, this study documents the specific response of rice under defined conditions, and illustrates that the plasticity of plant response to mild stress is complex and highly context-dependent, even under greenhouse conditions in which other potential environmental stress impacts are minimized.
25615402	13	17	dep	complex	2261:2267	arg1	plasticity					2214:2223	the plasticity	2210:2223	the plasticity of plant response to mild stress	2210:2256	Overall, this study documents the specific response of rice under defined conditions, and illustrates that the plasticity of plant response to mild stress is complex and highly context-dependent, even under greenhouse conditions in which other potential environmental stress impacts are minimized.
25615402	13	17	dep	complex	2261:2267	arg1	context-dependent					2280:2296	context-dependent	2280:2296	context-dependent	2280:2296	Overall, this study documents the specific response of rice under defined conditions, and illustrates that the plasticity of plant response to mild stress is complex and highly context-dependent, even under greenhouse conditions in which other potential environmental stress impacts are minimized.
25615402	12	18	theme	functional	1952:1961	arg1	features					1963:1970	compositional and functional features	1934:1970	compositional and functional features	1934:1970	This similarity of compositional and functional features was supported by multivariate analysis of all variables measured, which suggested that differences due to treatments were minimal.
25615402	7	19	theme	stimulatory	1166:1176	arg1	effect					1178:1183	a stimulatory effect	1164:1183	a stimulatory effect of salt on starch accumulation	1164:1214	(ii) Grain starch increased in Seedling EC4 (32.6%) and Anthesis EC2 (39%), respectively, suggesting a stimulatory effect of salt on starch accumulation.
25615402	4	20	from	processes	697:705	arg1	observation					637:647	observation	637:647	observation of the isolated effect of salt on grain filling processes	637:705	The former simulates field conditions while the latter permits observation of the isolated effect of salt on grain filling processes.
25615402	8	21	theme	salinity	1227:1234	arg1	treatment					1236:1244	The salinity treatment	1223:1244	The salinity treatment of 2 dS/m(2)	1223:1257	(iii) The salinity treatment of 2 dS/m(2) was better tolerated at anthesis than the 4 dS/m(2) treatment as the latter led to reduced grain weight (28.8%) and seed fertility (19.4%) and compensatory increases in protein (20.1%) and nitrogen (19.8%) contents.
25615402	11	22	theme	EC4	1900:1902	arg1	treatment					1904:1912	the Anthesis EC4 treatment	1887:1912	the Anthesis EC4 treatment	1887:1912	The only alterations in starch were limited to small changes in thermal properties and glucan chain distribution, which were only seen in the Anthesis EC4 treatment.
25615402	8	23	from	weight	1356:1361	arg1	%					1462:1462	19.8%	1458:1462	19.8%	1458:1462	(iii) The salinity treatment of 2 dS/m(2) was better tolerated at anthesis than the 4 dS/m(2) treatment as the latter led to reduced grain weight (28.8%) and seed fertility (19.4%) and compensatory increases in protein (20.1%) and nitrogen (19.8%) contents.
25615402	8	23	from	weight	1356:1361	arg1	protein					1428:1434	protein (20.1%) and nitrogen (19.8%) contents	1428:1472	protein	1428:1434	(iii) The salinity treatment of 2 dS/m(2) was better tolerated at anthesis than the 4 dS/m(2) treatment as the latter led to reduced grain weight (28.8%) and seed fertility (19.4%) and compensatory increases in protein (20.1%) and nitrogen (19.8%) contents.
25615402	8	23	from	weight	1356:1361	arg1	contents					1465:1472	protein (20.1%) and nitrogen (19.8%) contents	1428:1472	contents	1465:1472	(iii) The salinity treatment of 2 dS/m(2) was better tolerated at anthesis than the 4 dS/m(2) treatment as the latter led to reduced grain weight (28.8%) and seed fertility (19.4%) and compensatory increases in protein (20.1%) and nitrogen (19.8%) contents.
25615402	8	23	from	weight	1356:1361	arg1	%					1441:1441	20.1%	1437:1441	20.1%	1437:1441	(iii) The salinity treatment of 2 dS/m(2) was better tolerated at anthesis than the 4 dS/m(2) treatment as the latter led to reduced grain weight (28.8%) and seed fertility (19.4%) and compensatory increases in protein (20.1%) and nitrogen (19.8%) contents.
25615402	4	24	theme	field	595:599	arg1	conditions					601:610	field conditions	595:610	field conditions	595:610	The former simulates field conditions while the latter permits observation of the isolated effect of salt on grain filling processes.
25615402	5	25	dep	following	743:751	arg1	showed					765:770	showed	765:770	showed adaptive responses to prolonged salt treatment with no negative effects on grain weight or fertility	765:871	Key findings of this study are the following: (i) Plants showed adaptive responses to prolonged salt treatment with no negative effects on grain weight or fertility.
25615402	5	26	theme	adaptive	772:779	arg1	responses					781:789	adaptive responses	772:789	adaptive responses to prolonged salt treatment with no negative effects on grain weight or fertility	772:871	Key findings of this study are the following: (i) Plants showed adaptive responses to prolonged salt treatment with no negative effects on grain weight or fertility.
25615402	11	27	theme	chain	1843:1847	arg1	distribution					1849:1860	glucan chain distribution	1836:1860	glucan chain distribution	1836:1860	The only alterations in starch were limited to small changes in thermal properties and glucan chain distribution, which were only seen in the Anthesis EC4 treatment.
25615402	8	28	theme	dS/m	1251:1254	arg1	treatment					1236:1244	The salinity treatment	1223:1244	The salinity treatment of 2 dS/m(2)	1223:1257	(iii) The salinity treatment of 2 dS/m(2) was better tolerated at anthesis than the 4 dS/m(2) treatment as the latter led to reduced grain weight (28.8%) and seed fertility (19.4%) and compensatory increases in protein (20.1%) and nitrogen (19.8%) contents.
25615402	6	29	theme	agronomic	1032:1040	arg1	parameters					1042:1051	the agronomic parameters	1028:1051	the agronomic parameters measured	1028:1060	Seedling EC2 plants had more panicles and enhanced caryopsis dimensions, while surprisingly, Seedling EC4 plants did not differ from the control group in the agronomic parameters measured.
25615402	13	30	theme	response	2234:2241	arg1	complex					2261:2267	complex	2261:2267	complex	2261:2267	Overall, this study documents the specific response of rice under defined conditions, and illustrates that the plasticity of plant response to mild stress is complex and highly context-dependent, even under greenhouse conditions in which other potential environmental stress impacts are minimized.
25615402	13	30	theme	response	2234:2241	arg1	plasticity					2214:2223	the plasticity	2210:2223	the plasticity of plant response to mild stress	2210:2256	Overall, this study documents the specific response of rice under defined conditions, and illustrates that the plasticity of plant response to mild stress is complex and highly context-dependent, even under greenhouse conditions in which other potential environmental stress impacts are minimized.
25615402	13	30	theme	response	2234:2241	arg1	context-dependent					2280:2296	context-dependent	2280:2296	context-dependent	2280:2296	Overall, this study documents the specific response of rice under defined conditions, and illustrates that the plasticity of plant response to mild stress is complex and highly context-dependent, even under greenhouse conditions in which other potential environmental stress impacts are minimized.
25615402	4	31	from	effect	665:670	arg1	processes					697:705	grain filling processes	683:705	grain filling processes	683:705	The former simulates field conditions while the latter permits observation of the isolated effect of salt on grain filling processes.
25615402	3	32	dep	transplant	463:472	arg1	EC4					499:501	EC4	499:501	EC4	499:501	Plants were grown in sodium chloride-containing soil (2 or 4 dS/m(2) electrical conductivity), which was imposed 4-weeks after transplant (called Seedling EC2 and EC4) or after the appearance of the anthers (called Anthesis EC2 and EC4).
25615402	3	32	dep	transplant	463:472	arg1	EC2					491:493	Seedling EC2	482:493	Seedling EC2	482:493	Plants were grown in sodium chloride-containing soil (2 or 4 dS/m(2) electrical conductivity), which was imposed 4-weeks after transplant (called Seedling EC2 and EC4) or after the appearance of the anthers (called Anthesis EC2 and EC4).
25615402	0	33	from	Effects	0:6	arg1	composition					89:99	grain composition	83:99	grain composition	83:99	Effects of timing and severity of salinity stress on rice (Oryza sativa L.) yield, grain composition, and starch functionality.
25615402	0	33	from	Effects	0:6	arg1	functionality					113:125	starch functionality	106:125	starch functionality	106:125	Effects of timing and severity of salinity stress on rice (Oryza sativa L.) yield, grain composition, and starch functionality.
25615402	0	33	from	Effects	0:6	arg1	yield					76:80	rice (Oryza sativa L.) yield	53:80	rice (Oryza sativa L.) yield	53:80	Effects of timing and severity of salinity stress on rice (Oryza sativa L.) yield, grain composition, and starch functionality.
25615402	5	34	dep	showed	765:770	arg1	i					755:755	i	755:755	i	755:755	Key findings of this study are the following: (i) Plants showed adaptive responses to prolonged salt treatment with no negative effects on grain weight or fertility.
25615402	13	35	theme	mild	2246:2249	arg1	stress					2251:2256	mild stress	2246:2256	mild stress	2246:2256	Overall, this study documents the specific response of rice under defined conditions, and illustrates that the plasticity of plant response to mild stress is complex and highly context-dependent, even under greenhouse conditions in which other potential environmental stress impacts are minimized.
25615402	4	36	from	observation	637:647	arg1	processes					697:705	grain filling processes	683:705	grain filling processes	683:705	The former simulates field conditions while the latter permits observation of the isolated effect of salt on grain filling processes.
25615402	3	37	theme	chloride-containing	364:382	arg1	dS/m					397:400	2 or 4 dS/m(2) electrical conductivity	390:427	2 or 4 dS/m(2) electrical conductivity	390:427	Plants were grown in sodium chloride-containing soil (2 or 4 dS/m(2) electrical conductivity), which was imposed 4-weeks after transplant (called Seedling EC2 and EC4) or after the appearance of the anthers (called Anthesis EC2 and EC4).
25615402	3	37	theme	chloride-containing	364:382	arg1	soil					384:387	sodium chloride-containing soil	357:387	sodium chloride-containing soil (2 or 4 dS/m(2) electrical conductivity)	357:428	Plants were grown in sodium chloride-containing soil (2 or 4 dS/m(2) electrical conductivity), which was imposed 4-weeks after transplant (called Seedling EC2 and EC4) or after the appearance of the anthers (called Anthesis EC2 and EC4).
25615402	5	38	theme	negative	827:834	arg1	effects					836:842	no negative effects	824:842	no negative effects on grain weight or fertility	824:871	Key findings of this study are the following: (i) Plants showed adaptive responses to prolonged salt treatment with no negative effects on grain weight or fertility.
25615402	6	39	theme	enhanced	916:923	arg1	dimensions					935:944	enhanced caryopsis dimensions	916:944	enhanced caryopsis dimensions	916:944	Seedling EC2 plants had more panicles and enhanced caryopsis dimensions, while surprisingly, Seedling EC4 plants did not differ from the control group in the agronomic parameters measured.
25615402	12	40	theme	compositional	1934:1946	arg1	features					1963:1970	compositional and functional features	1934:1970	compositional and functional features	1934:1970	This similarity of compositional and functional features was supported by multivariate analysis of all variables measured, which suggested that differences due to treatments were minimal.
25615402	7	41	dep	starch	1074:1079	arg1	ii					1064:1065	ii	1064:1065	ii	1064:1065	(ii) Grain starch increased in Seedling EC4 (32.6%) and Anthesis EC2 (39%), respectively, suggesting a stimulatory effect of salt on starch accumulation.
25615402	3	42	dep	anthers	535:541	arg1	anthers					535:541	the anthers	531:541	the anthers (called Anthesis EC2 and EC4)	531:571	Plants were grown in sodium chloride-containing soil (2 or 4 dS/m(2) electrical conductivity), which was imposed 4-weeks after transplant (called Seedling EC2 and EC4) or after the appearance of the anthers (called Anthesis EC2 and EC4).
25615402	3	42	dep	anthers	535:541	arg1	EC4					568:570	EC4	568:570	EC4	568:570	Plants were grown in sodium chloride-containing soil (2 or 4 dS/m(2) electrical conductivity), which was imposed 4-weeks after transplant (called Seedling EC2 and EC4) or after the appearance of the anthers (called Anthesis EC2 and EC4).
25615402	3	42	dep	anthers	535:541	arg1	EC2					560:562	called Anthesis EC2	544:562	called Anthesis EC2	544:562	Plants were grown in sodium chloride-containing soil (2 or 4 dS/m(2) electrical conductivity), which was imposed 4-weeks after transplant (called Seedling EC2 and EC4) or after the appearance of the anthers (called Anthesis EC2 and EC4).
25615402	8	43	from	fertility	1380:1388	arg1	%					1462:1462	19.8%	1458:1462	19.8%	1458:1462	(iii) The salinity treatment of 2 dS/m(2) was better tolerated at anthesis than the 4 dS/m(2) treatment as the latter led to reduced grain weight (28.8%) and seed fertility (19.4%) and compensatory increases in protein (20.1%) and nitrogen (19.8%) contents.
25615402	8	43	from	fertility	1380:1388	arg1	protein					1428:1434	protein (20.1%) and nitrogen (19.8%) contents	1428:1472	protein	1428:1434	(iii) The salinity treatment of 2 dS/m(2) was better tolerated at anthesis than the 4 dS/m(2) treatment as the latter led to reduced grain weight (28.8%) and seed fertility (19.4%) and compensatory increases in protein (20.1%) and nitrogen (19.8%) contents.
25615402	8	43	from	fertility	1380:1388	arg1	contents					1465:1472	protein (20.1%) and nitrogen (19.8%) contents	1428:1472	contents	1465:1472	(iii) The salinity treatment of 2 dS/m(2) was better tolerated at anthesis than the 4 dS/m(2) treatment as the latter led to reduced grain weight (28.8%) and seed fertility (19.4%) and compensatory increases in protein (20.1%) and nitrogen (19.8%) contents.
25615402	8	43	from	fertility	1380:1388	arg1	%					1441:1441	20.1%	1437:1441	20.1%	1437:1441	(iii) The salinity treatment of 2 dS/m(2) was better tolerated at anthesis than the 4 dS/m(2) treatment as the latter led to reduced grain weight (28.8%) and seed fertility (19.4%) and compensatory increases in protein (20.1%) and nitrogen (19.8%) contents.
25615402	0	44	theme	timing	11:16	arg1	Effects					0:6	Effects	0:6	Effects of timing and severity of salinity stress on rice (Oryza sativa L.) yield, grain composition, and starch functionality.	0:126	Effects of timing and severity of salinity stress on rice (Oryza sativa L.) yield, grain composition, and starch functionality.
25615402	9	45	from	changes	1521:1527	arg1	content					1539:1545	starch content	1532:1545	starch content	1532:1545	(iv) Although some salinity treatments led to changes in starch content, these did not alter starch fine structure, morphology, or composition.
25615402	5	46	theme	salt	804:807	arg1	treatment					809:817	prolonged salt treatment	794:817	prolonged salt treatment with no negative effects on grain weight or fertility	794:871	Key findings of this study are the following: (i) Plants showed adaptive responses to prolonged salt treatment with no negative effects on grain weight or fertility.
25615402	7	47	theme	Anthesis	1119:1126	arg1	EC2					1128:1130	Anthesis EC2	1119:1130	Anthesis EC2 (39%)	1119:1136	(ii) Grain starch increased in Seedling EC4 (32.6%) and Anthesis EC2 (39%), respectively, suggesting a stimulatory effect of salt on starch accumulation.
25615402	7	47	theme	Anthesis	1119:1126	arg1	%					1135:1135	39%	1133:1135	39%	1133:1135	(ii) Grain starch increased in Seedling EC4 (32.6%) and Anthesis EC2 (39%), respectively, suggesting a stimulatory effect of salt on starch accumulation.
25615402	11	48	theme	thermal	1813:1819	arg1	properties					1821:1830	thermal properties	1813:1830	thermal properties	1813:1830	The only alterations in starch were limited to small changes in thermal properties and glucan chain distribution, which were only seen in the Anthesis EC4 treatment.
25615402	8	49	theme	dS/m	1303:1306	arg1	treatment					1311:1319	the 4 dS/m(2) treatment as the latter led to reduced grain weight (28.8%) and seed fertility (19.4%) and compensatory increases in protein (20.1%) and nitrogen (19.8%) contents	1297:1472	the 4 dS/m(2) treatment as the latter led to reduced grain weight (28.8%) and seed fertility (19.4%) and compensatory increases in protein (20.1%) and nitrogen (19.8%) contents	1297:1472	(iii) The salinity treatment of 2 dS/m(2) was better tolerated at anthesis than the 4 dS/m(2) treatment as the latter led to reduced grain weight (28.8%) and seed fertility (19.4%) and compensatory increases in protein (20.1%) and nitrogen (19.8%) contents.
25615402	3	50	theme	anthers	535:541	arg1	appearance					517:526	the appearance	513:526	the appearance of the anthers (called Anthesis EC2 and EC4)	513:571	Plants were grown in sodium chloride-containing soil (2 or 4 dS/m(2) electrical conductivity), which was imposed 4-weeks after transplant (called Seedling EC2 and EC4) or after the appearance of the anthers (called Anthesis EC2 and EC4).
25615402	4	51	theme	grain	683:687	arg1	processes					697:705	grain filling processes	683:705	grain filling processes	683:705	The former simulates field conditions while the latter permits observation of the isolated effect of salt on grain filling processes.
25615402	0	52	theme	severity	22:29	arg1	Effects					0:6	Effects	0:6	Effects of timing and severity of salinity stress on rice (Oryza sativa L.) yield, grain composition, and starch functionality.	0:126	Effects of timing and severity of salinity stress on rice (Oryza sativa L.) yield, grain composition, and starch functionality.
25615402	6	53	theme	EC2	883:885	arg1	plants					887:892	Seedling EC2 plants	874:892	Seedling EC2 plants	874:892	Seedling EC2 plants had more panicles and enhanced caryopsis dimensions, while surprisingly, Seedling EC4 plants did not differ from the control group in the agronomic parameters measured.
25615402	0	54	theme	salinity	34:41	arg1	stress					43:48	salinity stress	34:48	salinity stress	34:48	Effects of timing and severity of salinity stress on rice (Oryza sativa L.) yield, grain composition, and starch functionality.
25615402	6	55	theme	EC4	976:978	arg1	plants					980:985	Seedling EC4 plants	967:985	Seedling EC4 plants	967:985	Seedling EC2 plants had more panicles and enhanced caryopsis dimensions, while surprisingly, Seedling EC4 plants did not differ from the control group in the agronomic parameters measured.
25615402	9	56	theme	salinity	1494:1501	arg1	treatments					1503:1512	some salinity treatments	1489:1512	some salinity treatments	1489:1512	(iv) Although some salinity treatments led to changes in starch content, these did not alter starch fine structure, morphology, or composition.
25615402	4	57	theme	effect	665:670	arg1	observation					637:647	observation	637:647	observation of the isolated effect of salt on grain filling processes	637:705	The former simulates field conditions while the latter permits observation of the isolated effect of salt on grain filling processes.
25615402	9	58	dep	alter	1562:1566	arg1	iv					1476:1477	iv	1476:1477	iv	1476:1477	(iv) Although some salinity treatments led to changes in starch content, these did not alter starch fine structure, morphology, or composition.
25615402	10	59	theme	starch	1687:1692	arg1	distribution					1707:1718	starch granule size distribution	1687:1718	starch granule size distribution	1687:1718	We observed no differences in reducing sugar and amylose content or starch granule size distribution among any of the treatments.
25615402	5	60	from	effects	836:842	arg1	fertility					863:871	fertility	863:871	fertility	863:871	Key findings of this study are the following: (i) Plants showed adaptive responses to prolonged salt treatment with no negative effects on grain weight or fertility.
25615402	5	60	from	effects	836:842	arg1	weight					853:858	grain weight	847:858	grain weight	847:858	Key findings of this study are the following: (i) Plants showed adaptive responses to prolonged salt treatment with no negative effects on grain weight or fertility.
25615402	3	61	theme	called	544:549	arg1	anthers					535:541	the anthers	531:541	the anthers (called Anthesis EC2 and EC4)	531:571	Plants were grown in sodium chloride-containing soil (2 or 4 dS/m(2) electrical conductivity), which was imposed 4-weeks after transplant (called Seedling EC2 and EC4) or after the appearance of the anthers (called Anthesis EC2 and EC4).
25615402	3	61	theme	called	544:549	arg1	EC2					560:562	called Anthesis EC2	544:562	called Anthesis EC2	544:562	Plants were grown in sodium chloride-containing soil (2 or 4 dS/m(2) electrical conductivity), which was imposed 4-weeks after transplant (called Seedling EC2 and EC4) or after the appearance of the anthers (called Anthesis EC2 and EC4).
25615402	4	62	theme	salt	675:678	arg1	effect					665:670	the isolated effect	652:670	the isolated effect of salt on grain filling processes	652:705	The former simulates field conditions while the latter permits observation of the isolated effect of salt on grain filling processes.
25615402	2	63	theme	salinity	301:308	arg1	stress					310:315	low-to-moderate salinity stress	285:315	low-to-moderate salinity stress in the greenhouse	285:333	Nipponbare) grains from plants grown under low-to-moderate salinity stress in the greenhouse.
25615402	3	64	theme	electrical	405:414	arg1	conductivity					416:427	electrical conductivity	405:427	2 or 4 dS/m(2) electrical conductivity	390:427	Plants were grown in sodium chloride-containing soil (2 or 4 dS/m(2) electrical conductivity), which was imposed 4-weeks after transplant (called Seedling EC2 and EC4) or after the appearance of the anthers (called Anthesis EC2 and EC4).
25615402	8	65	theme	seed	1375:1378	arg1	fertility					1380:1388	seed fertility	1375:1388	seed fertility (19.4%)	1375:1396	(iii) The salinity treatment of 2 dS/m(2) was better tolerated at anthesis than the 4 dS/m(2) treatment as the latter led to reduced grain weight (28.8%) and seed fertility (19.4%) and compensatory increases in protein (20.1%) and nitrogen (19.8%) contents.
25615402	8	65	theme	seed	1375:1378	arg1	%					1395:1395	19.4%	1391:1395	19.4%	1391:1395	(iii) The salinity treatment of 2 dS/m(2) was better tolerated at anthesis than the 4 dS/m(2) treatment as the latter led to reduced grain weight (28.8%) and seed fertility (19.4%) and compensatory increases in protein (20.1%) and nitrogen (19.8%) contents.
25615402	9	66	theme	starch	1532:1537	arg1	content					1539:1545	starch content	1532:1545	starch content	1532:1545	(iv) Although some salinity treatments led to changes in starch content, these did not alter starch fine structure, morphology, or composition.
25615402	8	67	theme	reduced	1342:1348	arg1	weight					1356:1361	reduced grain weight	1342:1361	reduced grain weight (28.8%)	1342:1369	(iii) The salinity treatment of 2 dS/m(2) was better tolerated at anthesis than the 4 dS/m(2) treatment as the latter led to reduced grain weight (28.8%) and seed fertility (19.4%) and compensatory increases in protein (20.1%) and nitrogen (19.8%) contents.
25615402	8	67	theme	reduced	1342:1348	arg1	%					1368:1368	28.8%	1364:1368	28.8%	1364:1368	(iii) The salinity treatment of 2 dS/m(2) was better tolerated at anthesis than the 4 dS/m(2) treatment as the latter led to reduced grain weight (28.8%) and seed fertility (19.4%) and compensatory increases in protein (20.1%) and nitrogen (19.8%) contents.
25615402	2	68	from	plants	266:271	arg1	grains					254:259	grains	254:259	grains from plants	254:271	Nipponbare) grains from plants grown under low-to-moderate salinity stress in the greenhouse.
25615402	13	69	theme	specific	2137:2144	arg1	response					2146:2153	the specific response	2133:2153	the specific response of rice	2133:2161	Overall, this study documents the specific response of rice under defined conditions, and illustrates that the plasticity of plant response to mild stress is complex and highly context-dependent, even under greenhouse conditions in which other potential environmental stress impacts are minimized.
25615402	13	70	theme	other	2341:2345	arg1	impacts					2378:2384	other potential environmental stress impacts	2341:2384	other potential environmental stress impacts	2341:2384	Overall, this study documents the specific response of rice under defined conditions, and illustrates that the plasticity of plant response to mild stress is complex and highly context-dependent, even under greenhouse conditions in which other potential environmental stress impacts are minimized.
25615402	7	71	theme	starch	1196:1201	arg1	accumulation					1203:1214	starch accumulation	1196:1214	starch accumulation	1196:1214	(ii) Grain starch increased in Seedling EC4 (32.6%) and Anthesis EC2 (39%), respectively, suggesting a stimulatory effect of salt on starch accumulation.
25615402	11	72	from	alterations	1758:1768	arg1	starch					1773:1778	starch	1773:1778	starch	1773:1778	The only alterations in starch were limited to small changes in thermal properties and glucan chain distribution, which were only seen in the Anthesis EC4 treatment.
25615402	0	73	theme	grain	83:87	arg1	composition					89:99	grain composition	83:99	grain composition	83:99	Effects of timing and severity of salinity stress on rice (Oryza sativa L.) yield, grain composition, and starch functionality.
25615402	1	74	theme	work	144:147	arg1	aim					132:134	The aim	128:134	The aim of this work	128:147	The aim of this work was to examine agronomic, compositional, and functional changes in rice (Oryza sativa L. cv.
25615402	12	75	theme	features	1963:1970	arg1	similarity					1920:1929	This similarity	1915:1929	This similarity of compositional and functional features	1915:1970	This similarity of compositional and functional features was supported by multivariate analysis of all variables measured, which suggested that differences due to treatments were minimal.
25615402	7	76	theme	salt	1188:1191	arg1	effect					1178:1183	a stimulatory effect	1164:1183	a stimulatory effect of salt on starch accumulation	1164:1214	(ii) Grain starch increased in Seedling EC4 (32.6%) and Anthesis EC2 (39%), respectively, suggesting a stimulatory effect of salt on starch accumulation.
25615402	9	77	theme	fine	1575:1578	arg1	structure					1580:1588	starch fine structure	1568:1588	starch fine structure	1568:1588	(iv) Although some salinity treatments led to changes in starch content, these did not alter starch fine structure, morphology, or composition.
25615402	13	78	theme	greenhouse	2310:2319	arg1	conditions					2321:2330	greenhouse conditions	2310:2330	greenhouse conditions in which other potential environmental stress impacts are minimized	2310:2398	Overall, this study documents the specific response of rice under defined conditions, and illustrates that the plasticity of plant response to mild stress is complex and highly context-dependent, even under greenhouse conditions in which other potential environmental stress impacts are minimized.
25615402	10	79	theme	size	1702:1705	arg1	distribution					1707:1718	starch granule size distribution	1687:1718	starch granule size distribution	1687:1718	We observed no differences in reducing sugar and amylose content or starch granule size distribution among any of the treatments.
25615402	5	80	with	treatment	809:817	arg1	effects					836:842	no negative effects	824:842	no negative effects on grain weight or fertility	824:871	Key findings of this study are the following: (i) Plants showed adaptive responses to prolonged salt treatment with no negative effects on grain weight or fertility.
25615402	0	81	theme	starch	106:111	arg1	functionality					113:125	starch functionality	106:125	starch functionality	106:125	Effects of timing and severity of salinity stress on rice (Oryza sativa L.) yield, grain composition, and starch functionality.
25615402	3	82	theme	Seedling	482:489	arg1	EC2					491:493	Seedling EC2	482:493	Seedling EC2	482:493	Plants were grown in sodium chloride-containing soil (2 or 4 dS/m(2) electrical conductivity), which was imposed 4-weeks after transplant (called Seedling EC2 and EC4) or after the appearance of the anthers (called Anthesis EC2 and EC4).
25615402	1	83	from	changes	205:211	arg1	rice					216:219	rice	216:219	rice	216:219	The aim of this work was to examine agronomic, compositional, and functional changes in rice (Oryza sativa L. cv.
25615402	1	84	from	agronomic	164:172	arg1	rice					216:219	rice	216:219	rice	216:219	The aim of this work was to examine agronomic, compositional, and functional changes in rice (Oryza sativa L. cv.
25615402	6	85	theme	Seedling	874:881	arg1	plants					887:892	Seedling EC2 plants	874:892	Seedling EC2 plants	874:892	Seedling EC2 plants had more panicles and enhanced caryopsis dimensions, while surprisingly, Seedling EC4 plants did not differ from the control group in the agronomic parameters measured.
25615402	6	86	theme	Seedling	967:974	arg1	plants					980:985	Seedling EC4 plants	967:985	Seedling EC4 plants	967:985	Seedling EC2 plants had more panicles and enhanced caryopsis dimensions, while surprisingly, Seedling EC4 plants did not differ from the control group in the agronomic parameters measured.
25615402	0	87	theme	rice	53:56	arg1	yield					76:80	rice (Oryza sativa L.) yield	53:80	rice (Oryza sativa L.) yield	53:80	Effects of timing and severity of salinity stress on rice (Oryza sativa L.) yield, grain composition, and starch functionality.
25615402	8	88	theme	nitrogen	1448:1455	arg1	contents					1465:1472	protein (20.1%) and nitrogen (19.8%) contents	1428:1472	contents	1465:1472	(iii) The salinity treatment of 2 dS/m(2) was better tolerated at anthesis than the 4 dS/m(2) treatment as the latter led to reduced grain weight (28.8%) and seed fertility (19.4%) and compensatory increases in protein (20.1%) and nitrogen (19.8%) contents.
25615402	8	88	theme	nitrogen	1448:1455	arg1	%					1462:1462	19.8%	1458:1462	19.8%	1458:1462	(iii) The salinity treatment of 2 dS/m(2) was better tolerated at anthesis than the 4 dS/m(2) treatment as the latter led to reduced grain weight (28.8%) and seed fertility (19.4%) and compensatory increases in protein (20.1%) and nitrogen (19.8%) contents.
25615402	1	89	dep	Oryza	222:226	arg1	cv					238:239	cv	238:239	cv	238:239	The aim of this work was to examine agronomic, compositional, and functional changes in rice (Oryza sativa L. cv.
25615402	1	89	dep	Oryza	222:226	arg1	L.					235:236	Oryza sativa L.	222:236	Oryza sativa L. cv	222:239	The aim of this work was to examine agronomic, compositional, and functional changes in rice (Oryza sativa L. cv.
25615402	11	90	theme	glucan	1836:1841	arg1	distribution					1849:1860	glucan chain distribution	1836:1860	glucan chain distribution	1836:1860	The only alterations in starch were limited to small changes in thermal properties and glucan chain distribution, which were only seen in the Anthesis EC4 treatment.
25615402	10	91	theme	amylose	1668:1674	arg1	content					1676:1682	sugar and amylose content	1658:1682	content	1676:1682	We observed no differences in reducing sugar and amylose content or starch granule size distribution among any of the treatments.
25615402	1	92	dep	examine	156:162	arg1	Oryza					222:226	Oryza	222:226	Oryza	222:226	The aim of this work was to examine agronomic, compositional, and functional changes in rice (Oryza sativa L. cv.
25615402	3	93	dep	dS/m	397:400	arg1	2					402:402	2	402:402	2	402:402	Plants were grown in sodium chloride-containing soil (2 or 4 dS/m(2) electrical conductivity), which was imposed 4-weeks after transplant (called Seedling EC2 and EC4) or after the appearance of the anthers (called Anthesis EC2 and EC4).
25615402	3	93	dep	dS/m	397:400	arg1	conductivity					416:427	electrical conductivity	405:427	2 or 4 dS/m(2) electrical conductivity	390:427	Plants were grown in sodium chloride-containing soil (2 or 4 dS/m(2) electrical conductivity), which was imposed 4-weeks after transplant (called Seedling EC2 and EC4) or after the appearance of the anthers (called Anthesis EC2 and EC4).
25615402	2	94	dep	grown	273:277	arg1	Nipponbare					242:251	Nipponbare	242:251	Nipponbare	242:251	Nipponbare) grains from plants grown under low-to-moderate salinity stress in the greenhouse.
25615402	10	95	theme	sugar	1658:1662	arg1	content					1676:1682	sugar and amylose content	1658:1682	content	1676:1682	We observed no differences in reducing sugar and amylose content or starch granule size distribution among any of the treatments.
25615402	6	96	theme	caryopsis	925:933	arg1	dimensions					935:944	enhanced caryopsis dimensions	916:944	enhanced caryopsis dimensions	916:944	Seedling EC2 plants had more panicles and enhanced caryopsis dimensions, while surprisingly, Seedling EC4 plants did not differ from the control group in the agronomic parameters measured.
25615402	6	97	contain	had	894:896	arg2	dimensions					935:944	enhanced caryopsis dimensions	916:944	enhanced caryopsis dimensions	916:944	Seedling EC2 plants had more panicles and enhanced caryopsis dimensions, while surprisingly, Seedling EC4 plants did not differ from the control group in the agronomic parameters measured.
25615402	6	97	contain	had	894:896	arg1	plants					887:892	Seedling EC2 plants	874:892	Seedling EC2 plants	874:892	Seedling EC2 plants had more panicles and enhanced caryopsis dimensions, while surprisingly, Seedling EC4 plants did not differ from the control group in the agronomic parameters measured.
25615402	6	97	contain	had	894:896	arg2	panicles					903:910	more panicles	898:910	more panicles	898:910	Seedling EC2 plants had more panicles and enhanced caryopsis dimensions, while surprisingly, Seedling EC4 plants did not differ from the control group in the agronomic parameters measured.
25615402	0	98	dep	Oryza	59:63	arg1	L.					72:73	Oryza sativa L.	59:73	Oryza sativa L.	59:73	Effects of timing and severity of salinity stress on rice (Oryza sativa L.) yield, grain composition, and starch functionality.
25615402	11	99	theme	small	1796:1800	arg1	changes					1802:1808	small changes	1796:1808	small changes	1796:1808	The only alterations in starch were limited to small changes in thermal properties and glucan chain distribution, which were only seen in the Anthesis EC4 treatment.
25615402	3	100	theme	sodium	357:362	arg1	dS/m					397:400	2 or 4 dS/m(2) electrical conductivity	390:427	2 or 4 dS/m(2) electrical conductivity	390:427	Plants were grown in sodium chloride-containing soil (2 or 4 dS/m(2) electrical conductivity), which was imposed 4-weeks after transplant (called Seedling EC2 and EC4) or after the appearance of the anthers (called Anthesis EC2 and EC4).
25615402	3	100	theme	sodium	357:362	arg1	soil					384:387	sodium chloride-containing soil	357:387	sodium chloride-containing soil (2 or 4 dS/m(2) electrical conductivity)	357:428	Plants were grown in sodium chloride-containing soil (2 or 4 dS/m(2) electrical conductivity), which was imposed 4-weeks after transplant (called Seedling EC2 and EC4) or after the appearance of the anthers (called Anthesis EC2 and EC4).
25615402	13	101	theme	plant	2228:2232	arg1	response					2234:2241	plant response	2228:2241	plant response to mild stress	2228:2256	Overall, this study documents the specific response of rice under defined conditions, and illustrates that the plasticity of plant response to mild stress is complex and highly context-dependent, even under greenhouse conditions in which other potential environmental stress impacts are minimized.
25615402	5	102	theme	prolonged	794:802	arg1	treatment					809:817	prolonged salt treatment	794:817	prolonged salt treatment with no negative effects on grain weight or fertility	794:871	Key findings of this study are the following: (i) Plants showed adaptive responses to prolonged salt treatment with no negative effects on grain weight or fertility.
25615402	6	103	theme	more	898:901	arg1	panicles					903:910	more panicles	898:910	more panicles	898:910	Seedling EC2 plants had more panicles and enhanced caryopsis dimensions, while surprisingly, Seedling EC4 plants did not differ from the control group in the agronomic parameters measured.
25615402	7	104	from	effect	1178:1183	arg1	accumulation					1203:1214	starch accumulation	1196:1214	starch accumulation	1196:1214	(ii) Grain starch increased in Seedling EC4 (32.6%) and Anthesis EC2 (39%), respectively, suggesting a stimulatory effect of salt on starch accumulation.
25615402	0	105	theme	stress	43:48	arg1	timing					11:16	timing	11:16	timing	11:16	Effects of timing and severity of salinity stress on rice (Oryza sativa L.) yield, grain composition, and starch functionality.
25615402	0	105	theme	stress	43:48	arg1	severity					22:29	severity	22:29	severity	22:29	Effects of timing and severity of salinity stress on rice (Oryza sativa L.) yield, grain composition, and starch functionality.
25615402	2	106	from	stress	310:315	arg1	greenhouse					324:333	the greenhouse	320:333	the greenhouse	320:333	Nipponbare) grains from plants grown under low-to-moderate salinity stress in the greenhouse.
25615402	3	107	theme	Anthesis	551:558	arg1	anthers					535:541	the anthers	531:541	the anthers (called Anthesis EC2 and EC4)	531:571	Plants were grown in sodium chloride-containing soil (2 or 4 dS/m(2) electrical conductivity), which was imposed 4-weeks after transplant (called Seedling EC2 and EC4) or after the appearance of the anthers (called Anthesis EC2 and EC4).
25615402	3	107	theme	Anthesis	551:558	arg1	EC2					560:562	called Anthesis EC2	544:562	called Anthesis EC2	544:562	Plants were grown in sodium chloride-containing soil (2 or 4 dS/m(2) electrical conductivity), which was imposed 4-weeks after transplant (called Seedling EC2 and EC4) or after the appearance of the anthers (called Anthesis EC2 and EC4).
25615402	4	108	theme	filling	689:695	arg1	processes					697:705	grain filling processes	683:705	grain filling processes	683:705	The former simulates field conditions while the latter permits observation of the isolated effect of salt on grain filling processes.
25615402	7	109	theme	Seedling	1094:1101	arg1	EC4					1103:1105	Seedling EC4	1094:1105	Seedling EC4 (32.6%)	1094:1113	(ii) Grain starch increased in Seedling EC4 (32.6%) and Anthesis EC2 (39%), respectively, suggesting a stimulatory effect of salt on starch accumulation.
25615402	7	109	theme	Seedling	1094:1101	arg1	%					1112:1112	32.6%	1108:1112	32.6%	1108:1112	(ii) Grain starch increased in Seedling EC4 (32.6%) and Anthesis EC2 (39%), respectively, suggesting a stimulatory effect of salt on starch accumulation.
25615402	10	110	theme	granule	1694:1700	arg1	distribution					1707:1718	starch granule size distribution	1687:1718	starch granule size distribution	1687:1718	We observed no differences in reducing sugar and amylose content or starch granule size distribution among any of the treatments.
25615402	12	111	theme	variables	2018:2026	arg1	analysis					2002:2009	multivariate analysis	1989:2009	multivariate analysis	1989:2009	This similarity of compositional and functional features was supported by multivariate analysis of all variables measured, which suggested that differences due to treatments were minimal.
25615402	4	112	theme	isolated	656:663	arg1	effect					665:670	the isolated effect	652:670	the isolated effect of salt on grain filling processes	652:705	The former simulates field conditions while the latter permits observation of the isolated effect of salt on grain filling processes.
25615402	5	113	theme	grain	847:851	arg1	weight					853:858	grain weight	847:858	grain weight	847:858	Key findings of this study are the following: (i) Plants showed adaptive responses to prolonged salt treatment with no negative effects on grain weight or fertility.
25615402	11	114	from	changes	1802:1808	arg1	distribution					1849:1860	glucan chain distribution	1836:1860	glucan chain distribution	1836:1860	The only alterations in starch were limited to small changes in thermal properties and glucan chain distribution, which were only seen in the Anthesis EC4 treatment.
25615402	11	114	from	changes	1802:1808	arg1	properties					1821:1830	thermal properties	1813:1830	thermal properties	1813:1830	The only alterations in starch were limited to small changes in thermal properties and glucan chain distribution, which were only seen in the Anthesis EC4 treatment.
25615402	8	115	from	increases	1415:1423	arg1	%					1462:1462	19.8%	1458:1462	19.8%	1458:1462	(iii) The salinity treatment of 2 dS/m(2) was better tolerated at anthesis than the 4 dS/m(2) treatment as the latter led to reduced grain weight (28.8%) and seed fertility (19.4%) and compensatory increases in protein (20.1%) and nitrogen (19.8%) contents.
25615402	8	115	from	increases	1415:1423	arg1	protein					1428:1434	protein (20.1%) and nitrogen (19.8%) contents	1428:1472	protein	1428:1434	(iii) The salinity treatment of 2 dS/m(2) was better tolerated at anthesis than the 4 dS/m(2) treatment as the latter led to reduced grain weight (28.8%) and seed fertility (19.4%) and compensatory increases in protein (20.1%) and nitrogen (19.8%) contents.
25615402	8	115	from	increases	1415:1423	arg1	contents					1465:1472	protein (20.1%) and nitrogen (19.8%) contents	1428:1472	contents	1465:1472	(iii) The salinity treatment of 2 dS/m(2) was better tolerated at anthesis than the 4 dS/m(2) treatment as the latter led to reduced grain weight (28.8%) and seed fertility (19.4%) and compensatory increases in protein (20.1%) and nitrogen (19.8%) contents.
25615402	8	115	from	increases	1415:1423	arg1	%					1441:1441	20.1%	1437:1441	20.1%	1437:1441	(iii) The salinity treatment of 2 dS/m(2) was better tolerated at anthesis than the 4 dS/m(2) treatment as the latter led to reduced grain weight (28.8%) and seed fertility (19.4%) and compensatory increases in protein (20.1%) and nitrogen (19.8%) contents.
25615402	2	116	theme	low-to-moderate	285:299	arg1	stress					310:315	low-to-moderate salinity stress	285:315	low-to-moderate salinity stress in the greenhouse	285:333	Nipponbare) grains from plants grown under low-to-moderate salinity stress in the greenhouse.
25615402	13	117	theme	potential	2347:2355	arg1	impacts					2378:2384	other potential environmental stress impacts	2341:2384	other potential environmental stress impacts	2341:2384	Overall, this study documents the specific response of rice under defined conditions, and illustrates that the plasticity of plant response to mild stress is complex and highly context-dependent, even under greenhouse conditions in which other potential environmental stress impacts are minimized.
25615402	8	118	dep	tolerated	1270:1278	arg1	iii					1218:1220	iii	1218:1220	iii	1218:1220	(iii) The salinity treatment of 2 dS/m(2) was better tolerated at anthesis than the 4 dS/m(2) treatment as the latter led to reduced grain weight (28.8%) and seed fertility (19.4%) and compensatory increases in protein (20.1%) and nitrogen (19.8%) contents.
23836845	6	0	theme	tissue	1515:1520	arg1	engineering					1522:1532	tissue engineering	1515:1532	tissue engineering of bone	1515:1540	The in situ hardening calcium phosphate cement-alginate microcarriers developed herein may be used as potential three-dimensional scaffolds for cell delivery and tissue engineering of bone.
23836845	3	1	from	process	812:818	arg1	water					775:779	water	775:779	water	775:779	Moreover, a porous structure could be easily created in the solid microcarriers by soaking the produced microcarriers in water and a subsequent freeze-drying process.
23836845	1	2	theme	calcium	163:169	arg1	phosphate					171:179	calcium phosphate	163:179	calcium phosphate cement	163:186	Novel microcarriers consisting of calcium phosphate cement and alginate were prepared for use as three-dimensional scaffolds for the culture and expansion of cells that are effective for bone tissue engineering.
23836845	2	3	from	hardening	601:609	arg1	bath					569:572	an oil bath	562:572	an oil bath	562:572	The calcium phosphate cement-alginate composite microcarriers were produced by an emulsification of the composite aqueous solutions mixed at varying ratios (calcium phosphate cement powder/alginate solution = 0.8-1.2) in an oil bath and the subsequent in situ hardening of the compositions during spherodization.
23836845	2	4	theme	cement	516:521	arg1	powder/alginate					523:537	calcium phosphate cement powder/alginate solution = 0.8-1.2	498:556	calcium phosphate cement powder/alginate solution = 0.8-1.2	498:556	The calcium phosphate cement-alginate composite microcarriers were produced by an emulsification of the composite aqueous solutions mixed at varying ratios (calcium phosphate cement powder/alginate solution = 0.8-1.2) in an oil bath and the subsequent in situ hardening of the compositions during spherodization.
23836845	2	4	theme	cement	516:521	arg1	ratios					490:495	varying ratios	482:495	varying ratios (calcium phosphate cement powder/alginate solution = 0.8-1.2) in an oil bath	482:572	The calcium phosphate cement-alginate composite microcarriers were produced by an emulsification of the composite aqueous solutions mixed at varying ratios (calcium phosphate cement powder/alginate solution = 0.8-1.2) in an oil bath and the subsequent in situ hardening of the compositions during spherodization.
23836845	6	5	theme	cement-alginate	1393:1407	arg1	scaffolds					1483:1491	potential three-dimensional scaffolds	1455:1491	potential three-dimensional scaffolds for cell delivery and tissue engineering of bone	1455:1540	The in situ hardening calcium phosphate cement-alginate microcarriers developed herein may be used as potential three-dimensional scaffolds for cell delivery and tissue engineering of bone.
23836845	6	5	theme	cement-alginate	1393:1407	arg1	microcarriers					1409:1421	The in situ hardening calcium phosphate cement-alginate microcarriers	1353:1421	The in situ hardening calcium phosphate cement-alginate microcarriers developed herein	1353:1438	The in situ hardening calcium phosphate cement-alginate microcarriers developed herein may be used as potential three-dimensional scaffolds for cell delivery and tissue engineering of bone.
23836845	1	6	theme	phosphate	171:179	arg1	cement					181:186	calcium phosphate cement	163:186	calcium phosphate cement	163:186	Novel microcarriers consisting of calcium phosphate cement and alginate were prepared for use as three-dimensional scaffolds for the culture and expansion of cells that are effective for bone tissue engineering.
23836845	3	7	theme	porous	666:671	arg1	structure					673:681	a porous structure	664:681	a porous structure	664:681	Moreover, a porous structure could be easily created in the solid microcarriers by soaking the produced microcarriers in water and a subsequent freeze-drying process.
23836845	6	8	theme	phosphate	1383:1391	arg1	scaffolds					1483:1491	potential three-dimensional scaffolds	1455:1491	potential three-dimensional scaffolds for cell delivery and tissue engineering of bone	1455:1540	The in situ hardening calcium phosphate cement-alginate microcarriers developed herein may be used as potential three-dimensional scaffolds for cell delivery and tissue engineering of bone.
23836845	6	8	theme	phosphate	1383:1391	arg1	microcarriers					1409:1421	The in situ hardening calcium phosphate cement-alginate microcarriers	1353:1421	The in situ hardening calcium phosphate cement-alginate microcarriers developed herein	1353:1438	The in situ hardening calcium phosphate cement-alginate microcarriers developed herein may be used as potential three-dimensional scaffolds for cell delivery and tissue engineering of bone.
23836845	6	9	theme	cell	1497:1500	arg1	delivery					1502:1509	cell delivery	1497:1509	cell delivery	1497:1509	The in situ hardening calcium phosphate cement-alginate microcarriers developed herein may be used as potential three-dimensional scaffolds for cell delivery and tissue engineering of bone.
23836845	2	10	theme	oil	565:567	arg1	bath					569:572	an oil bath	562:572	an oil bath	562:572	The calcium phosphate cement-alginate composite microcarriers were produced by an emulsification of the composite aqueous solutions mixed at varying ratios (calcium phosphate cement powder/alginate solution = 0.8-1.2) in an oil bath and the subsequent in situ hardening of the compositions during spherodization.
23836845	2	11	theme	compositions	618:629	arg1	hardening					601:609	the subsequent in situ hardening	578:609	the subsequent in situ hardening of the compositions during spherodization	578:651	The calcium phosphate cement-alginate composite microcarriers were produced by an emulsification of the composite aqueous solutions mixed at varying ratios (calcium phosphate cement powder/alginate solution = 0.8-1.2) in an oil bath and the subsequent in situ hardening of the compositions during spherodization.
23836845	2	11	theme	compositions	618:629	arg1	powder/alginate					523:537	calcium phosphate cement powder/alginate solution = 0.8-1.2	498:556	calcium phosphate cement powder/alginate solution = 0.8-1.2	498:556	The calcium phosphate cement-alginate composite microcarriers were produced by an emulsification of the composite aqueous solutions mixed at varying ratios (calcium phosphate cement powder/alginate solution = 0.8-1.2) in an oil bath and the subsequent in situ hardening of the compositions during spherodization.
23836845	2	11	theme	compositions	618:629	arg1	ratios					490:495	varying ratios	482:495	varying ratios (calcium phosphate cement powder/alginate solution = 0.8-1.2) in an oil bath	482:572	The calcium phosphate cement-alginate composite microcarriers were produced by an emulsification of the composite aqueous solutions mixed at varying ratios (calcium phosphate cement powder/alginate solution = 0.8-1.2) in an oil bath and the subsequent in situ hardening of the compositions during spherodization.
23836845	6	12	theme	calcium	1375:1381	arg1	scaffolds					1483:1491	potential three-dimensional scaffolds	1455:1491	potential three-dimensional scaffolds for cell delivery and tissue engineering of bone	1455:1540	The in situ hardening calcium phosphate cement-alginate microcarriers developed herein may be used as potential three-dimensional scaffolds for cell delivery and tissue engineering of bone.
23836845	6	12	theme	calcium	1375:1381	arg1	microcarriers					1409:1421	The in situ hardening calcium phosphate cement-alginate microcarriers	1353:1421	The in situ hardening calcium phosphate cement-alginate microcarriers developed herein	1353:1438	The in situ hardening calcium phosphate cement-alginate microcarriers developed herein may be used as potential three-dimensional scaffolds for cell delivery and tissue engineering of bone.
23836845	2	13	theme	composite	379:387	arg1	microcarriers					389:401	The calcium phosphate cement-alginate composite microcarriers	341:401	The calcium phosphate cement-alginate composite microcarriers	341:401	The calcium phosphate cement-alginate composite microcarriers were produced by an emulsification of the composite aqueous solutions mixed at varying ratios (calcium phosphate cement powder/alginate solution = 0.8-1.2) in an oil bath and the subsequent in situ hardening of the compositions during spherodization.
23836845	4	14	from	phase	1024:1028	arg1	cement					1055:1060	the calcium phosphate cement	1033:1060	the calcium phosphate cement	1033:1060	Bone mineral-like apatite nanocrystallites were shown to rapidly develop on the calcium phosphate cement-alginate microcarriers under moist conditions due to the conversion of the α-tricalcium phosphate phase in the calcium phosphate cement into a carbonate-hydroxyapatite.
23836845	0	15	theme	bone	110:113	arg1	regeneration					115:126	bone regeneration	110:126	bone regeneration	110:126	Preparation of in situ hardening composite microcarriers: calcium phosphate cement combined with alginate for bone regeneration.
23836845	2	16	from	ratios	490:495	arg1	bath					569:572	an oil bath	562:572	an oil bath	562:572	The calcium phosphate cement-alginate composite microcarriers were produced by an emulsification of the composite aqueous solutions mixed at varying ratios (calcium phosphate cement powder/alginate solution = 0.8-1.2) in an oil bath and the subsequent in situ hardening of the compositions during spherodization.
23836845	4	17	theme	calcium	901:907	arg1	microcarriers					935:947	the calcium phosphate cement-alginate microcarriers	897:947	the calcium phosphate cement-alginate microcarriers	897:947	Bone mineral-like apatite nanocrystallites were shown to rapidly develop on the calcium phosphate cement-alginate microcarriers under moist conditions due to the conversion of the α-tricalcium phosphate phase in the calcium phosphate cement into a carbonate-hydroxyapatite.
23836845	4	18	theme	mineral-like	826:837	arg1	nanocrystallites					847:862	Bone mineral-like apatite nanocrystallites	821:862	Bone mineral-like apatite nanocrystallites	821:862	Bone mineral-like apatite nanocrystallites were shown to rapidly develop on the calcium phosphate cement-alginate microcarriers under moist conditions due to the conversion of the α-tricalcium phosphate phase in the calcium phosphate cement into a carbonate-hydroxyapatite.
23836845	4	19	theme	apatite	839:845	arg1	nanocrystallites					847:862	Bone mineral-like apatite nanocrystallites	821:862	Bone mineral-like apatite nanocrystallites	821:862	Bone mineral-like apatite nanocrystallites were shown to rapidly develop on the calcium phosphate cement-alginate microcarriers under moist conditions due to the conversion of the α-tricalcium phosphate phase in the calcium phosphate cement into a carbonate-hydroxyapatite.
23836845	6	20	theme	bone	1537:1540	arg1	delivery					1502:1509	cell delivery	1497:1509	cell delivery	1497:1509	The in situ hardening calcium phosphate cement-alginate microcarriers developed herein may be used as potential three-dimensional scaffolds for cell delivery and tissue engineering of bone.
23836845	6	20	theme	bone	1537:1540	arg1	engineering					1522:1532	tissue engineering	1515:1532	tissue engineering of bone	1515:1540	The in situ hardening calcium phosphate cement-alginate microcarriers developed herein may be used as potential three-dimensional scaffolds for cell delivery and tissue engineering of bone.
23836845	3	21	theme	produced	749:756	arg1	microcarriers					758:770	the produced microcarriers	745:770	the produced microcarriers in water	745:779	Moreover, a porous structure could be easily created in the solid microcarriers by soaking the produced microcarriers in water and a subsequent freeze-drying process.
23836845	0	22	theme	composite	33:41	arg1	microcarriers					43:55	in situ hardening composite microcarriers	15:55	in situ hardening composite microcarriers	15:55	Preparation of in situ hardening composite microcarriers: calcium phosphate cement combined with alginate for bone regeneration.
23836845	4	23	theme	Bone	821:824	arg1	nanocrystallites					847:862	Bone mineral-like apatite nanocrystallites	821:862	Bone mineral-like apatite nanocrystallites	821:862	Bone mineral-like apatite nanocrystallites were shown to rapidly develop on the calcium phosphate cement-alginate microcarriers under moist conditions due to the conversion of the α-tricalcium phosphate phase in the calcium phosphate cement into a carbonate-hydroxyapatite.
23836845	6	24	theme	three-dimensional	1465:1481	arg1	scaffolds					1483:1491	potential three-dimensional scaffolds	1455:1491	potential three-dimensional scaffolds for cell delivery and tissue engineering of bone	1455:1540	The in situ hardening calcium phosphate cement-alginate microcarriers developed herein may be used as potential three-dimensional scaffolds for cell delivery and tissue engineering of bone.
23836845	6	24	theme	three-dimensional	1465:1481	arg1	microcarriers					1409:1421	The in situ hardening calcium phosphate cement-alginate microcarriers	1353:1421	The in situ hardening calcium phosphate cement-alginate microcarriers developed herein	1353:1438	The in situ hardening calcium phosphate cement-alginate microcarriers developed herein may be used as potential three-dimensional scaffolds for cell delivery and tissue engineering of bone.
23836845	2	25	dep	in	593:594	arg1	situ					596:599	situ	596:599	situ	596:599	The calcium phosphate cement-alginate composite microcarriers were produced by an emulsification of the composite aqueous solutions mixed at varying ratios (calcium phosphate cement powder/alginate solution = 0.8-1.2) in an oil bath and the subsequent in situ hardening of the compositions during spherodization.
23836845	2	26	theme	varying	482:488	arg1	powder/alginate					523:537	calcium phosphate cement powder/alginate solution = 0.8-1.2	498:556	calcium phosphate cement powder/alginate solution = 0.8-1.2	498:556	The calcium phosphate cement-alginate composite microcarriers were produced by an emulsification of the composite aqueous solutions mixed at varying ratios (calcium phosphate cement powder/alginate solution = 0.8-1.2) in an oil bath and the subsequent in situ hardening of the compositions during spherodization.
23836845	2	26	theme	varying	482:488	arg1	ratios					490:495	varying ratios	482:495	varying ratios (calcium phosphate cement powder/alginate solution = 0.8-1.2) in an oil bath	482:572	The calcium phosphate cement-alginate composite microcarriers were produced by an emulsification of the composite aqueous solutions mixed at varying ratios (calcium phosphate cement powder/alginate solution = 0.8-1.2) in an oil bath and the subsequent in situ hardening of the compositions during spherodization.
23836845	4	27	theme	cement-alginate	919:933	arg1	microcarriers					935:947	the calcium phosphate cement-alginate microcarriers	897:947	the calcium phosphate cement-alginate microcarriers	897:947	Bone mineral-like apatite nanocrystallites were shown to rapidly develop on the calcium phosphate cement-alginate microcarriers under moist conditions due to the conversion of the α-tricalcium phosphate phase in the calcium phosphate cement into a carbonate-hydroxyapatite.
23836845	3	28	theme	subsequent	787:796	arg1	process					812:818	a subsequent freeze-drying process	785:818	a subsequent freeze-drying process	785:818	Moreover, a porous structure could be easily created in the solid microcarriers by soaking the produced microcarriers in water and a subsequent freeze-drying process.
23836845	5	29	theme	active	1189:1194	arg1	potential					1210:1218	an active proliferative potential	1186:1218	an active proliferative potential during 14 days of culture	1186:1244	Osteoblastic cells cultured on the microspherical scaffolds were proven to be viable, with an active proliferative potential during 14 days of culture, and their osteogenic differentiation was confirmed by the determination of alkaline phosphatase activity.
23836845	3	30	from	microcarriers	758:770	arg1	water					775:779	water	775:779	water	775:779	Moreover, a porous structure could be easily created in the solid microcarriers by soaking the produced microcarriers in water and a subsequent freeze-drying process.
23836845	1	31	theme	cells	287:291	arg1	expansion					274:282	expansion	274:282	expansion	274:282	Novel microcarriers consisting of calcium phosphate cement and alginate were prepared for use as three-dimensional scaffolds for the culture and expansion of cells that are effective for bone tissue engineering.
23836845	1	31	theme	cells	287:291	arg1	culture					262:268	culture	262:268	culture	262:268	Novel microcarriers consisting of calcium phosphate cement and alginate were prepared for use as three-dimensional scaffolds for the culture and expansion of cells that are effective for bone tissue engineering.
23836845	4	32	theme	phosphate	909:917	arg1	microcarriers					935:947	the calcium phosphate cement-alginate microcarriers	897:947	the calcium phosphate cement-alginate microcarriers	897:947	Bone mineral-like apatite nanocrystallites were shown to rapidly develop on the calcium phosphate cement-alginate microcarriers under moist conditions due to the conversion of the α-tricalcium phosphate phase in the calcium phosphate cement into a carbonate-hydroxyapatite.
23836845	6	33	used	used	1447:1450	arg2	microcarriers					1409:1421	The in situ hardening calcium phosphate cement-alginate microcarriers	1353:1421	The in situ hardening calcium phosphate cement-alginate microcarriers developed herein	1353:1438	The in situ hardening calcium phosphate cement-alginate microcarriers developed herein may be used as potential three-dimensional scaffolds for cell delivery and tissue engineering of bone.
23836845	6	33	used	used	1447:1450	arg2	scaffolds					1483:1491	potential three-dimensional scaffolds	1455:1491	potential three-dimensional scaffolds for cell delivery and tissue engineering of bone	1455:1540	The in situ hardening calcium phosphate cement-alginate microcarriers developed herein may be used as potential three-dimensional scaffolds for cell delivery and tissue engineering of bone.
23836845	6	34	theme	hardening	1365:1373	arg1	scaffolds					1483:1491	potential three-dimensional scaffolds	1455:1491	potential three-dimensional scaffolds for cell delivery and tissue engineering of bone	1455:1540	The in situ hardening calcium phosphate cement-alginate microcarriers developed herein may be used as potential three-dimensional scaffolds for cell delivery and tissue engineering of bone.
23836845	6	34	theme	hardening	1365:1373	arg1	microcarriers					1409:1421	The in situ hardening calcium phosphate cement-alginate microcarriers	1353:1421	The in situ hardening calcium phosphate cement-alginate microcarriers developed herein	1353:1438	The in situ hardening calcium phosphate cement-alginate microcarriers developed herein may be used as potential three-dimensional scaffolds for cell delivery and tissue engineering of bone.
23836845	6	35	theme	potential	1455:1463	arg1	scaffolds					1483:1491	potential three-dimensional scaffolds	1455:1491	potential three-dimensional scaffolds for cell delivery and tissue engineering of bone	1455:1540	The in situ hardening calcium phosphate cement-alginate microcarriers developed herein may be used as potential three-dimensional scaffolds for cell delivery and tissue engineering of bone.
23836845	6	35	theme	potential	1455:1463	arg1	microcarriers					1409:1421	The in situ hardening calcium phosphate cement-alginate microcarriers	1353:1421	The in situ hardening calcium phosphate cement-alginate microcarriers developed herein	1353:1438	The in situ hardening calcium phosphate cement-alginate microcarriers developed herein may be used as potential three-dimensional scaffolds for cell delivery and tissue engineering of bone.
23836845	3	36	theme	freeze-drying	798:810	arg1	process					812:818	a subsequent freeze-drying process	785:818	a subsequent freeze-drying process	785:818	Moreover, a porous structure could be easily created in the solid microcarriers by soaking the produced microcarriers in water and a subsequent freeze-drying process.
23836845	2	37	theme	in	593:594	arg1	hardening					601:609	the subsequent in situ hardening	578:609	the subsequent in situ hardening of the compositions during spherodization	578:651	The calcium phosphate cement-alginate composite microcarriers were produced by an emulsification of the composite aqueous solutions mixed at varying ratios (calcium phosphate cement powder/alginate solution = 0.8-1.2) in an oil bath and the subsequent in situ hardening of the compositions during spherodization.
23836845	2	38	theme	aqueous	455:461	arg1	solutions					463:471	the composite aqueous solutions	441:471	the composite aqueous solutions mixed at varying ratios (calcium phosphate cement powder/alginate solution = 0.8-1.2) in an oil bath and the subsequent in situ hardening of the compositions during spherodization	441:651	The calcium phosphate cement-alginate composite microcarriers were produced by an emulsification of the composite aqueous solutions mixed at varying ratios (calcium phosphate cement powder/alginate solution = 0.8-1.2) in an oil bath and the subsequent in situ hardening of the compositions during spherodization.
23836845	0	39	theme	in	15:16	arg1	microcarriers					43:55	in situ hardening composite microcarriers	15:55	in situ hardening composite microcarriers	15:55	Preparation of in situ hardening composite microcarriers: calcium phosphate cement combined with alginate for bone regeneration.
23836845	2	40	theme	subsequent	582:591	arg1	hardening					601:609	the subsequent in situ hardening	578:609	the subsequent in situ hardening of the compositions during spherodization	578:651	The calcium phosphate cement-alginate composite microcarriers were produced by an emulsification of the composite aqueous solutions mixed at varying ratios (calcium phosphate cement powder/alginate solution = 0.8-1.2) in an oil bath and the subsequent in situ hardening of the compositions during spherodization.
23836845	4	41	theme	α-tricalcium	1001:1012	arg1	phase					1024:1028	the α-tricalcium phosphate phase	997:1028	the α-tricalcium phosphate phase in the calcium phosphate cement	997:1060	Bone mineral-like apatite nanocrystallites were shown to rapidly develop on the calcium phosphate cement-alginate microcarriers under moist conditions due to the conversion of the α-tricalcium phosphate phase in the calcium phosphate cement into a carbonate-hydroxyapatite.
23836845	2	42	theme	cement-alginate	363:377	arg1	microcarriers					389:401	The calcium phosphate cement-alginate composite microcarriers	341:401	The calcium phosphate cement-alginate composite microcarriers	341:401	The calcium phosphate cement-alginate composite microcarriers were produced by an emulsification of the composite aqueous solutions mixed at varying ratios (calcium phosphate cement powder/alginate solution = 0.8-1.2) in an oil bath and the subsequent in situ hardening of the compositions during spherodization.
23836845	2	43	theme	composite	445:453	arg1	solutions					463:471	the composite aqueous solutions	441:471	the composite aqueous solutions mixed at varying ratios (calcium phosphate cement powder/alginate solution = 0.8-1.2) in an oil bath and the subsequent in situ hardening of the compositions during spherodization	441:651	The calcium phosphate cement-alginate composite microcarriers were produced by an emulsification of the composite aqueous solutions mixed at varying ratios (calcium phosphate cement powder/alginate solution = 0.8-1.2) in an oil bath and the subsequent in situ hardening of the compositions during spherodization.
23836845	2	44	theme	phosphate	506:514	arg1	powder/alginate					523:537	calcium phosphate cement powder/alginate solution = 0.8-1.2	498:556	calcium phosphate cement powder/alginate solution = 0.8-1.2	498:556	The calcium phosphate cement-alginate composite microcarriers were produced by an emulsification of the composite aqueous solutions mixed at varying ratios (calcium phosphate cement powder/alginate solution = 0.8-1.2) in an oil bath and the subsequent in situ hardening of the compositions during spherodization.
23836845	2	44	theme	phosphate	506:514	arg1	ratios					490:495	varying ratios	482:495	varying ratios (calcium phosphate cement powder/alginate solution = 0.8-1.2) in an oil bath	482:572	The calcium phosphate cement-alginate composite microcarriers were produced by an emulsification of the composite aqueous solutions mixed at varying ratios (calcium phosphate cement powder/alginate solution = 0.8-1.2) in an oil bath and the subsequent in situ hardening of the compositions during spherodization.
23836845	4	45	theme	phosphate	1014:1022	arg1	phase					1024:1028	the α-tricalcium phosphate phase	997:1028	the α-tricalcium phosphate phase in the calcium phosphate cement	997:1060	Bone mineral-like apatite nanocrystallites were shown to rapidly develop on the calcium phosphate cement-alginate microcarriers under moist conditions due to the conversion of the α-tricalcium phosphate phase in the calcium phosphate cement into a carbonate-hydroxyapatite.
23836845	5	46	with	viable	1173:1178	arg1	potential					1210:1218	an active proliferative potential	1186:1218	an active proliferative potential during 14 days of culture	1186:1244	Osteoblastic cells cultured on the microspherical scaffolds were proven to be viable, with an active proliferative potential during 14 days of culture, and their osteogenic differentiation was confirmed by the determination of alkaline phosphatase activity.
23836845	0	47	theme	hardening	23:31	arg1	microcarriers					43:55	in situ hardening composite microcarriers	15:55	in situ hardening composite microcarriers	15:55	Preparation of in situ hardening composite microcarriers: calcium phosphate cement combined with alginate for bone regeneration.
23836845	5	48	theme	culture	1238:1244	arg1	days					1230:1233	14 days	1227:1233	14 days of culture	1227:1244	Osteoblastic cells cultured on the microspherical scaffolds were proven to be viable, with an active proliferative potential during 14 days of culture, and their osteogenic differentiation was confirmed by the determination of alkaline phosphatase activity.
23836845	2	49	theme	calcium	498:504	arg1	powder/alginate					523:537	calcium phosphate cement powder/alginate solution = 0.8-1.2	498:556	calcium phosphate cement powder/alginate solution = 0.8-1.2	498:556	The calcium phosphate cement-alginate composite microcarriers were produced by an emulsification of the composite aqueous solutions mixed at varying ratios (calcium phosphate cement powder/alginate solution = 0.8-1.2) in an oil bath and the subsequent in situ hardening of the compositions during spherodization.
23836845	2	49	theme	calcium	498:504	arg1	ratios					490:495	varying ratios	482:495	varying ratios (calcium phosphate cement powder/alginate solution = 0.8-1.2) in an oil bath	482:572	The calcium phosphate cement-alginate composite microcarriers were produced by an emulsification of the composite aqueous solutions mixed at varying ratios (calcium phosphate cement powder/alginate solution = 0.8-1.2) in an oil bath and the subsequent in situ hardening of the compositions during spherodization.
23836845	5	50	theme	activity	1343:1350	arg1	determination					1305:1317	the determination	1301:1317	the determination of alkaline phosphatase activity	1301:1350	Osteoblastic cells cultured on the microspherical scaffolds were proven to be viable, with an active proliferative potential during 14 days of culture, and their osteogenic differentiation was confirmed by the determination of alkaline phosphatase activity.
23836845	1	51	theme	bone	316:319	arg1	engineering					328:338	bone tissue engineering	316:338	bone tissue engineering	316:338	Novel microcarriers consisting of calcium phosphate cement and alginate were prepared for use as three-dimensional scaffolds for the culture and expansion of cells that are effective for bone tissue engineering.
23836845	4	52	theme	phosphate	1045:1053	arg1	cement					1055:1060	the calcium phosphate cement	1033:1060	the calcium phosphate cement	1033:1060	Bone mineral-like apatite nanocrystallites were shown to rapidly develop on the calcium phosphate cement-alginate microcarriers under moist conditions due to the conversion of the α-tricalcium phosphate phase in the calcium phosphate cement into a carbonate-hydroxyapatite.
23836845	5	53	theme	proliferative	1196:1208	arg1	potential					1210:1218	an active proliferative potential	1186:1218	an active proliferative potential during 14 days of culture	1186:1244	Osteoblastic cells cultured on the microspherical scaffolds were proven to be viable, with an active proliferative potential during 14 days of culture, and their osteogenic differentiation was confirmed by the determination of alkaline phosphatase activity.
23836845	2	54	theme	phosphate	353:361	arg1	microcarriers					389:401	The calcium phosphate cement-alginate composite microcarriers	341:401	The calcium phosphate cement-alginate composite microcarriers	341:401	The calcium phosphate cement-alginate composite microcarriers were produced by an emulsification of the composite aqueous solutions mixed at varying ratios (calcium phosphate cement powder/alginate solution = 0.8-1.2) in an oil bath and the subsequent in situ hardening of the compositions during spherodization.
23836845	0	55	theme	microcarriers	43:55	arg1	Preparation					0:10	Preparation	0:10	Preparation of in situ hardening composite microcarriers: calcium phosphate cement combined with alginate for bone regeneration.	0:127	Preparation of in situ hardening composite microcarriers: calcium phosphate cement combined with alginate for bone regeneration.
23836845	1	56	theme	tissue	321:326	arg1	engineering					328:338	bone tissue engineering	316:338	bone tissue engineering	316:338	Novel microcarriers consisting of calcium phosphate cement and alginate were prepared for use as three-dimensional scaffolds for the culture and expansion of cells that are effective for bone tissue engineering.
23836845	4	57	theme	due	972:974	arg1	conditions					961:970	moist conditions	955:970	moist conditions due to the conversion of the α-tricalcium phosphate phase in the calcium phosphate cement into a carbonate-hydroxyapatite	955:1092	Bone mineral-like apatite nanocrystallites were shown to rapidly develop on the calcium phosphate cement-alginate microcarriers under moist conditions due to the conversion of the α-tricalcium phosphate phase in the calcium phosphate cement into a carbonate-hydroxyapatite.
23836845	4	58	from	conversion	983:992	arg1	cement					1055:1060	the calcium phosphate cement	1033:1060	the calcium phosphate cement	1033:1060	Bone mineral-like apatite nanocrystallites were shown to rapidly develop on the calcium phosphate cement-alginate microcarriers under moist conditions due to the conversion of the α-tricalcium phosphate phase in the calcium phosphate cement into a carbonate-hydroxyapatite.
23836845	5	59	theme	microspherical	1130:1143	arg1	scaffolds					1145:1153	the microspherical scaffolds	1126:1153	the microspherical scaffolds	1126:1153	Osteoblastic cells cultured on the microspherical scaffolds were proven to be viable, with an active proliferative potential during 14 days of culture, and their osteogenic differentiation was confirmed by the determination of alkaline phosphatase activity.
23836845	6	60	dep	in	1357:1358	arg1	situ					1360:1363	situ	1360:1363	situ	1360:1363	The in situ hardening calcium phosphate cement-alginate microcarriers developed herein may be used as potential three-dimensional scaffolds for cell delivery and tissue engineering of bone.
23836845	2	61	theme	calcium	345:351	arg1	microcarriers					389:401	The calcium phosphate cement-alginate composite microcarriers	341:401	The calcium phosphate cement-alginate composite microcarriers	341:401	The calcium phosphate cement-alginate composite microcarriers were produced by an emulsification of the composite aqueous solutions mixed at varying ratios (calcium phosphate cement powder/alginate solution = 0.8-1.2) in an oil bath and the subsequent in situ hardening of the compositions during spherodization.
23836845	4	62	theme	calcium	1037:1043	arg1	cement					1055:1060	the calcium phosphate cement	1033:1060	the calcium phosphate cement	1033:1060	Bone mineral-like apatite nanocrystallites were shown to rapidly develop on the calcium phosphate cement-alginate microcarriers under moist conditions due to the conversion of the α-tricalcium phosphate phase in the calcium phosphate cement into a carbonate-hydroxyapatite.
23836845	3	63	theme	solid	714:718	arg1	microcarriers					720:732	the solid microcarriers	710:732	the solid microcarriers	710:732	Moreover, a porous structure could be easily created in the solid microcarriers by soaking the produced microcarriers in water and a subsequent freeze-drying process.
23836845	6	64	theme	in	1357:1358	arg1	scaffolds					1483:1491	potential three-dimensional scaffolds	1455:1491	potential three-dimensional scaffolds for cell delivery and tissue engineering of bone	1455:1540	The in situ hardening calcium phosphate cement-alginate microcarriers developed herein may be used as potential three-dimensional scaffolds for cell delivery and tissue engineering of bone.
23836845	6	64	theme	in	1357:1358	arg1	microcarriers					1409:1421	The in situ hardening calcium phosphate cement-alginate microcarriers	1353:1421	The in situ hardening calcium phosphate cement-alginate microcarriers developed herein	1353:1438	The in situ hardening calcium phosphate cement-alginate microcarriers developed herein may be used as potential three-dimensional scaffolds for cell delivery and tissue engineering of bone.
23836845	1	65	theme	Novel	129:133	arg1	microcarriers					135:147	Novel microcarriers	129:147	Novel microcarriers consisting of calcium phosphate cement and alginate	129:199	Novel microcarriers consisting of calcium phosphate cement and alginate were prepared for use as three-dimensional scaffolds for the culture and expansion of cells that are effective for bone tissue engineering.
23836845	1	66	theme	three-dimensional	226:242	arg1	scaffolds					244:252	three-dimensional scaffolds	226:252	three-dimensional scaffolds for the culture and expansion of cells that are effective for bone tissue engineering	226:338	Novel microcarriers consisting of calcium phosphate cement and alginate were prepared for use as three-dimensional scaffolds for the culture and expansion of cells that are effective for bone tissue engineering.
23836845	1	67	dep	culture	262:268	arg1	the					258:260	the	258:260	the	258:260	Novel microcarriers consisting of calcium phosphate cement and alginate were prepared for use as three-dimensional scaffolds for the culture and expansion of cells that are effective for bone tissue engineering.
23836845	0	68	dep	in	15:16	arg1	situ					18:21	situ	18:21	situ	18:21	Preparation of in situ hardening composite microcarriers: calcium phosphate cement combined with alginate for bone regeneration.
23836845	4	69	theme	moist	955:959	arg1	conditions					961:970	moist conditions	955:970	moist conditions due to the conversion of the α-tricalcium phosphate phase in the calcium phosphate cement into a carbonate-hydroxyapatite	955:1092	Bone mineral-like apatite nanocrystallites were shown to rapidly develop on the calcium phosphate cement-alginate microcarriers under moist conditions due to the conversion of the α-tricalcium phosphate phase in the calcium phosphate cement into a carbonate-hydroxyapatite.
23836845	5	70	theme	phosphatase	1331:1341	arg1	activity					1343:1350	alkaline phosphatase activity	1322:1350	alkaline phosphatase activity	1322:1350	Osteoblastic cells cultured on the microspherical scaffolds were proven to be viable, with an active proliferative potential during 14 days of culture, and their osteogenic differentiation was confirmed by the determination of alkaline phosphatase activity.
23836845	0	71	theme	phosphate	66:74	arg1	cement					76:81	calcium phosphate cement	58:81	Preparation of in situ hardening composite microcarriers: calcium phosphate cement combined with alginate for bone regeneration.	0:127	Preparation of in situ hardening composite microcarriers: calcium phosphate cement combined with alginate for bone regeneration.
23836845	5	72	theme	Osteoblastic	1095:1106	arg1	cells					1108:1112	Osteoblastic cells	1095:1112	Osteoblastic cells cultured on the microspherical scaffolds	1095:1153	Osteoblastic cells cultured on the microspherical scaffolds were proven to be viable, with an active proliferative potential during 14 days of culture, and their osteogenic differentiation was confirmed by the determination of alkaline phosphatase activity.
23836845	2	73	theme	solutions	463:471	arg1	emulsification					423:436	an emulsification	420:436	an emulsification of the composite aqueous solutions mixed at varying ratios (calcium phosphate cement powder/alginate solution = 0.8-1.2) in an oil bath and the subsequent in situ hardening of the compositions during spherodization	420:651	The calcium phosphate cement-alginate composite microcarriers were produced by an emulsification of the composite aqueous solutions mixed at varying ratios (calcium phosphate cement powder/alginate solution = 0.8-1.2) in an oil bath and the subsequent in situ hardening of the compositions during spherodization.
23836845	5	74	theme	osteogenic	1257:1266	arg1	differentiation					1268:1282	their osteogenic differentiation	1251:1282	their osteogenic differentiation	1251:1282	Osteoblastic cells cultured on the microspherical scaffolds were proven to be viable, with an active proliferative potential during 14 days of culture, and their osteogenic differentiation was confirmed by the determination of alkaline phosphatase activity.
23836845	4	75	from	cement	1055:1060	arg1	conversion					983:992	the conversion	979:992	the conversion of the α-tricalcium phosphate phase in the calcium phosphate cement into a carbonate-hydroxyapatite	979:1092	Bone mineral-like apatite nanocrystallites were shown to rapidly develop on the calcium phosphate cement-alginate microcarriers under moist conditions due to the conversion of the α-tricalcium phosphate phase in the calcium phosphate cement into a carbonate-hydroxyapatite.
23836845	0	76	theme	calcium	58:64	arg1	cement					76:81	calcium phosphate cement	58:81	Preparation of in situ hardening composite microcarriers: calcium phosphate cement combined with alginate for bone regeneration.	0:127	Preparation of in situ hardening composite microcarriers: calcium phosphate cement combined with alginate for bone regeneration.
23836845	0	77	dep	Preparation	0:10	arg1	cement					76:81	calcium phosphate cement	58:81	Preparation of in situ hardening composite microcarriers: calcium phosphate cement combined with alginate for bone regeneration.	0:127	Preparation of in situ hardening composite microcarriers: calcium phosphate cement combined with alginate for bone regeneration.
23836845	5	78	theme	alkaline	1322:1329	arg1	phosphatase					1331:1341	alkaline phosphatase	1322:1341	alkaline phosphatase activity	1322:1350	Osteoblastic cells cultured on the microspherical scaffolds were proven to be viable, with an active proliferative potential during 14 days of culture, and their osteogenic differentiation was confirmed by the determination of alkaline phosphatase activity.
23836845	4	79	theme	phase	1024:1028	arg1	conversion					983:992	the conversion	979:992	the conversion of the α-tricalcium phosphate phase in the calcium phosphate cement into a carbonate-hydroxyapatite	979:1092	Bone mineral-like apatite nanocrystallites were shown to rapidly develop on the calcium phosphate cement-alginate microcarriers under moist conditions due to the conversion of the α-tricalcium phosphate phase in the calcium phosphate cement into a carbonate-hydroxyapatite.
26256164	4	0	theme	BZL	629:631	arg1	resonances					633:642	BZL resonances	629:642	BZL resonances characteristic of an amorphous drug	629:678	Conversely, BZL:methyl β-CD displayed BZL resonances characteristic of an amorphous drug and data analysis confirmed the presence of stable BZL:methyl β-CD inclusion complexes, with benzene encapsulated into the host cavity.
26256164	1	1	theme	CD	225:226	arg1	derivatives					229:239	cyclodextrin (CD) derivatives	211:239	cyclodextrin (CD) derivatives	211:239	Complexation of benznidazole (BZL), a drug of choice for the treatment of Chagas'neglected disease, with cyclodextrin (CD) derivatives was analyzed by solid-state NMR.
26256164	2	2	theme	β-CD	408:411	arg1	complexes					413:421	hydroxypropyl β-CD complexes	394:421	hydroxypropyl β-CD complexes	394:421	(13)C cross polarization/magic angle spinning spectra were recorded from BZL and from BZL:β-CD, BZL:methyl β-CD and BZL:hydroxypropyl β-CD complexes, which were obtained by the solvent evaporation technique.
26256164	7	3	theme	methyl	1050:1055	arg1	β-CD					1057:1060	methyl β-CD	1050:1060	methyl β-CD	1050:1060	The BZL interaction with methyl β-CD provided a route to stabilize amorphous BZL.
26256164	5	4	from	evidences	824:832	arg1	structure					845:853	complex structure	837:853	complex structure	837:853	Further evidences on complex structure and dynamics were obtained from proton and carbon spin-lattice relaxation times in the rotating frame.
26256164	5	4	from	evidences	824:832	arg1	dynamics					859:866	dynamics	859:866	dynamics	859:866	Further evidences on complex structure and dynamics were obtained from proton and carbon spin-lattice relaxation times in the rotating frame.
26256164	1	5	theme	choice	152:157	arg1	drug					144:147	a drug	142:147	a drug of choice for the treatment of Chagas'neglected disease	142:203	Complexation of benznidazole (BZL), a drug of choice for the treatment of Chagas'neglected disease, with cyclodextrin (CD) derivatives was analyzed by solid-state NMR.
26256164	1	5	theme	choice	152:157	arg1	benznidazole					122:133	benznidazole	122:133	benznidazole (BZL)	122:139	Complexation of benznidazole (BZL), a drug of choice for the treatment of Chagas'neglected disease, with cyclodextrin (CD) derivatives was analyzed by solid-state NMR.
26256164	7	6	theme	amorphous	1092:1100	arg1	BZL					1102:1104	amorphous BZL	1092:1104	amorphous BZL	1092:1104	The BZL interaction with methyl β-CD provided a route to stabilize amorphous BZL.
26256164	5	7	theme	Further	816:822	arg1	evidences					824:832	Further evidences	816:832	Further evidences on complex structure and dynamics	816:866	Further evidences on complex structure and dynamics were obtained from proton and carbon spin-lattice relaxation times in the rotating frame.
26256164	3	8	theme	significant	485:495	arg1	evidence					497:504	No significant evidence	482:504	No significant evidence	482:504	No significant evidence was obtained on BZL inclusion complexes involving either β-CD or hydroxypropyl β-CD.
26256164	4	9	theme	host	803:806	arg1	cavity					808:813	the host cavity	799:813	the host cavity	799:813	Conversely, BZL:methyl β-CD displayed BZL resonances characteristic of an amorphous drug and data analysis confirmed the presence of stable BZL:methyl β-CD inclusion complexes, with benzene encapsulated into the host cavity.
26256164	5	10	theme	carbon	898:903	arg1	times					929:933	proton and carbon spin-lattice relaxation times	887:933	times	929:933	Further evidences on complex structure and dynamics were obtained from proton and carbon spin-lattice relaxation times in the rotating frame.
26256164	7	11	with	interaction	1033:1043	arg1	β-CD					1057:1060	methyl β-CD	1050:1060	methyl β-CD	1050:1060	The BZL interaction with methyl β-CD provided a route to stabilize amorphous BZL.
26256164	8	12	theme	Physical	1107:1114	arg1	mixtures					1116:1123	Physical mixtures	1107:1123	Physical mixtures with identical BZL and CD compositions	1107:1162	Physical mixtures with identical BZL and CD compositions were also studied for comparison.
26256164	5	13	theme	complex	837:843	arg1	structure					845:853	complex structure	837:853	complex structure	837:853	Further evidences on complex structure and dynamics were obtained from proton and carbon spin-lattice relaxation times in the rotating frame.
26256164	7	14	theme	BZL	1029:1031	arg1	interaction					1033:1043	The BZL interaction	1025:1043	The BZL interaction with methyl β-CD	1025:1060	The BZL interaction with methyl β-CD provided a route to stabilize amorphous BZL.
26256164	2	15	dep	recorded	333:340	arg1	BZL					370:372	BZL	370:372	BZL	370:372	(13)C cross polarization/magic angle spinning spectra were recorded from BZL and from BZL:β-CD, BZL:methyl β-CD and BZL:hydroxypropyl β-CD complexes, which were obtained by the solvent evaporation technique.
26256164	2	15	dep	recorded	333:340	arg1	β-CD					364:367	β-CD	364:367	β-CD	364:367	(13)C cross polarization/magic angle spinning spectra were recorded from BZL and from BZL:β-CD, BZL:methyl β-CD and BZL:hydroxypropyl β-CD complexes, which were obtained by the solvent evaporation technique.
26256164	8	16	theme	identical	1130:1138	arg1	BZL					1140:1142	identical BZL	1130:1142	identical BZL	1130:1142	Physical mixtures with identical BZL and CD compositions were also studied for comparison.
26256164	8	17	theme	CD	1148:1149	arg1	compositions					1151:1162	CD compositions	1148:1162	CD compositions	1148:1162	Physical mixtures with identical BZL and CD compositions were also studied for comparison.
26256164	0	18	theme	First	0:4	arg1	evaluation					35:44	First solid-state NMR spectroscopy evaluation	0:44	First solid-state NMR spectroscopy evaluation of complexes of benznidazole with cyclodextrin derivatives.	0:104	First solid-state NMR spectroscopy evaluation of complexes of benznidazole with cyclodextrin derivatives.
26256164	5	19	from	times	929:933	arg1	frame					951:955	the rotating frame	938:955	the rotating frame	938:955	Further evidences on complex structure and dynamics were obtained from proton and carbon spin-lattice relaxation times in the rotating frame.
26256164	6	20	theme	spin	1009:1012	arg1	reservoir					1014:1022	a common guest-host spin reservoir	989:1022	a common guest-host spin reservoir	989:1022	These data are consistent with a common guest-host spin reservoir.
26256164	0	21	theme	solid-state	6:16	arg1	spectroscopy					22:33	solid-state NMR spectroscopy	6:33	First solid-state NMR spectroscopy evaluation of complexes of benznidazole with cyclodextrin derivatives.	0:104	First solid-state NMR spectroscopy evaluation of complexes of benznidazole with cyclodextrin derivatives.
26256164	0	22	theme	cyclodextrin	80:91	arg1	derivatives					93:103	cyclodextrin derivatives	80:103	cyclodextrin derivatives	80:103	First solid-state NMR spectroscopy evaluation of complexes of benznidazole with cyclodextrin derivatives.
26256164	4	23	theme	drug	675:678	arg1	characteristic					644:657	characteristic	644:657	characteristic	644:657	Conversely, BZL:methyl β-CD displayed BZL resonances characteristic of an amorphous drug and data analysis confirmed the presence of stable BZL:methyl β-CD inclusion complexes, with benzene encapsulated into the host cavity.
26256164	6	24	theme	guest-host	998:1007	arg1	reservoir					1014:1022	a common guest-host spin reservoir	989:1022	a common guest-host spin reservoir	989:1022	These data are consistent with a common guest-host spin reservoir.
26256164	2	25	theme	methyl	374:379	arg1	β-CD					381:384	methyl β-CD	374:384	methyl β-CD	374:384	(13)C cross polarization/magic angle spinning spectra were recorded from BZL and from BZL:β-CD, BZL:methyl β-CD and BZL:hydroxypropyl β-CD complexes, which were obtained by the solvent evaporation technique.
26256164	2	26	theme	13	275:276	arg1	C					278:278	C	278:278	C	278:278	(13)C cross polarization/magic angle spinning spectra were recorded from BZL and from BZL:β-CD, BZL:methyl β-CD and BZL:hydroxypropyl β-CD complexes, which were obtained by the solvent evaporation technique.
26256164	4	27	theme	BZL	603:605	arg1	β-CD					614:617	BZL:methyl β-CD	603:617	BZL:methyl β-CD	603:617	Conversely, BZL:methyl β-CD displayed BZL resonances characteristic of an amorphous drug and data analysis confirmed the presence of stable BZL:methyl β-CD inclusion complexes, with benzene encapsulated into the host cavity.
26256164	0	28	theme	spectroscopy	22:33	arg1	evaluation					35:44	First solid-state NMR spectroscopy evaluation	0:44	First solid-state NMR spectroscopy evaluation of complexes of benznidazole with cyclodextrin derivatives.	0:104	First solid-state NMR spectroscopy evaluation of complexes of benznidazole with cyclodextrin derivatives.
26256164	4	29	theme	amorphous	665:673	arg1	drug					675:678	an amorphous drug	662:678	an amorphous drug	662:678	Conversely, BZL:methyl β-CD displayed BZL resonances characteristic of an amorphous drug and data analysis confirmed the presence of stable BZL:methyl β-CD inclusion complexes, with benzene encapsulated into the host cavity.
26256164	4	30	theme	inclusion	747:755	arg1	complexes					757:765	stable BZL:methyl β-CD inclusion complexes	724:765	stable BZL:methyl β-CD inclusion complexes	724:765	Conversely, BZL:methyl β-CD displayed BZL resonances characteristic of an amorphous drug and data analysis confirmed the presence of stable BZL:methyl β-CD inclusion complexes, with benzene encapsulated into the host cavity.
26256164	4	31	theme	data	684:687	arg1	analysis					689:696	data analysis	684:696	data analysis	684:696	Conversely, BZL:methyl β-CD displayed BZL resonances characteristic of an amorphous drug and data analysis confirmed the presence of stable BZL:methyl β-CD inclusion complexes, with benzene encapsulated into the host cavity.
26256164	4	32	theme	β-CD	742:745	arg1	complexes					757:765	stable BZL:methyl β-CD inclusion complexes	724:765	stable BZL:methyl β-CD inclusion complexes	724:765	Conversely, BZL:methyl β-CD displayed BZL resonances characteristic of an amorphous drug and data analysis confirmed the presence of stable BZL:methyl β-CD inclusion complexes, with benzene encapsulated into the host cavity.
26256164	1	33	with	Complexation	106:117	arg1	derivatives					229:239	cyclodextrin (CD) derivatives	211:239	cyclodextrin (CD) derivatives	211:239	Complexation of benznidazole (BZL), a drug of choice for the treatment of Chagas'neglected disease, with cyclodextrin (CD) derivatives was analyzed by solid-state NMR.
26256164	1	34	theme	solid-state	257:267	arg1	NMR					269:271	solid-state NMR	257:271	solid-state NMR	257:271	Complexation of benznidazole (BZL), a drug of choice for the treatment of Chagas'neglected disease, with cyclodextrin (CD) derivatives was analyzed by solid-state NMR.
26256164	5	35	theme	spin-lattice	905:916	arg1	times					929:933	proton and carbon spin-lattice relaxation times	887:933	times	929:933	Further evidences on complex structure and dynamics were obtained from proton and carbon spin-lattice relaxation times in the rotating frame.
26256164	1	36	theme	benznidazole	122:133	arg1	Complexation					106:117	Complexation	106:117	Complexation of benznidazole (BZL), a drug of choice for the treatment of Chagas'neglected disease, with cyclodextrin (CD) derivatives	106:239	Complexation of benznidazole (BZL), a drug of choice for the treatment of Chagas'neglected disease, with cyclodextrin (CD) derivatives was analyzed by solid-state NMR.
26256164	2	37	theme	hydroxypropyl	394:406	arg1	complexes					413:421	hydroxypropyl β-CD complexes	394:421	hydroxypropyl β-CD complexes	394:421	(13)C cross polarization/magic angle spinning spectra were recorded from BZL and from BZL:β-CD, BZL:methyl β-CD and BZL:hydroxypropyl β-CD complexes, which were obtained by the solvent evaporation technique.
26256164	5	38	theme	rotating	942:949	arg1	frame					951:955	the rotating frame	938:955	the rotating frame	938:955	Further evidences on complex structure and dynamics were obtained from proton and carbon spin-lattice relaxation times in the rotating frame.
26256164	2	39	theme	evaporation	459:469	arg1	technique					471:479	the solvent evaporation technique	447:479	the solvent evaporation technique	447:479	(13)C cross polarization/magic angle spinning spectra were recorded from BZL and from BZL:β-CD, BZL:methyl β-CD and BZL:hydroxypropyl β-CD complexes, which were obtained by the solvent evaporation technique.
26256164	3	40	theme	BZL	522:524	arg1	complexes					536:544	BZL inclusion complexes	522:544	BZL inclusion complexes involving either β-CD or hydroxypropyl β-CD	522:588	No significant evidence was obtained on BZL inclusion complexes involving either β-CD or hydroxypropyl β-CD.
26256164	6	41	theme	common	991:996	arg1	reservoir					1014:1022	a common guest-host spin reservoir	989:1022	a common guest-host spin reservoir	989:1022	These data are consistent with a common guest-host spin reservoir.
26256164	1	42	theme	neglected	187:195	arg1	disease					197:203	Chagas'neglected disease	180:203	Chagas'neglected disease	180:203	Complexation of benznidazole (BZL), a drug of choice for the treatment of Chagas'neglected disease, with cyclodextrin (CD) derivatives was analyzed by solid-state NMR.
26256164	2	43	theme	solvent	451:457	arg1	technique					471:479	the solvent evaporation technique	447:479	the solvent evaporation technique	447:479	(13)C cross polarization/magic angle spinning spectra were recorded from BZL and from BZL:β-CD, BZL:methyl β-CD and BZL:hydroxypropyl β-CD complexes, which were obtained by the solvent evaporation technique.
26256164	3	44	theme	inclusion	526:534	arg1	complexes					536:544	BZL inclusion complexes	522:544	BZL inclusion complexes involving either β-CD or hydroxypropyl β-CD	522:588	No significant evidence was obtained on BZL inclusion complexes involving either β-CD or hydroxypropyl β-CD.
26256164	1	45	theme	disease	197:203	arg1	treatment					167:175	the treatment	163:175	the treatment of Chagas'neglected disease	163:203	Complexation of benznidazole (BZL), a drug of choice for the treatment of Chagas'neglected disease, with cyclodextrin (CD) derivatives was analyzed by solid-state NMR.
26256164	3	46	theme	hydroxypropyl	571:583	arg1	β-CD					585:588	hydroxypropyl β-CD	571:588	hydroxypropyl β-CD	571:588	No significant evidence was obtained on BZL inclusion complexes involving either β-CD or hydroxypropyl β-CD.
26256164	4	47	theme	complexes	757:765	arg1	presence					712:719	the presence	708:719	the presence of stable BZL:methyl β-CD inclusion complexes, with benzene encapsulated into the host cavity	708:813	Conversely, BZL:methyl β-CD displayed BZL resonances characteristic of an amorphous drug and data analysis confirmed the presence of stable BZL:methyl β-CD inclusion complexes, with benzene encapsulated into the host cavity.
26256164	4	48	theme	characteristic	644:657	arg1	resonances					633:642	BZL resonances	629:642	BZL resonances characteristic of an amorphous drug	629:678	Conversely, BZL:methyl β-CD displayed BZL resonances characteristic of an amorphous drug and data analysis confirmed the presence of stable BZL:methyl β-CD inclusion complexes, with benzene encapsulated into the host cavity.
26256164	0	49	theme	complexes	49:57	arg1	evaluation					35:44	First solid-state NMR spectroscopy evaluation	0:44	First solid-state NMR spectroscopy evaluation of complexes of benznidazole with cyclodextrin derivatives.	0:104	First solid-state NMR spectroscopy evaluation of complexes of benznidazole with cyclodextrin derivatives.
26256164	6	50	with	consistent	973:982	arg1	reservoir					1014:1022	a common guest-host spin reservoir	989:1022	a common guest-host spin reservoir	989:1022	These data are consistent with a common guest-host spin reservoir.
26256164	2	51	dep	β-CD	364:367	arg1	BZL					390:392	BZL	390:392	BZL	390:392	(13)C cross polarization/magic angle spinning spectra were recorded from BZL and from BZL:β-CD, BZL:methyl β-CD and BZL:hydroxypropyl β-CD complexes, which were obtained by the solvent evaporation technique.
26256164	2	51	dep	β-CD	364:367	arg1	β-CD					381:384	methyl β-CD	374:384	methyl β-CD	374:384	(13)C cross polarization/magic angle spinning spectra were recorded from BZL and from BZL:β-CD, BZL:methyl β-CD and BZL:hydroxypropyl β-CD complexes, which were obtained by the solvent evaporation technique.
26256164	2	51	dep	β-CD	364:367	arg1	complexes					413:421	hydroxypropyl β-CD complexes	394:421	hydroxypropyl β-CD complexes	394:421	(13)C cross polarization/magic angle spinning spectra were recorded from BZL and from BZL:β-CD, BZL:methyl β-CD and BZL:hydroxypropyl β-CD complexes, which were obtained by the solvent evaporation technique.
26256164	2	52	theme	polarization/magic	286:303	arg1	angle					305:309	(13)C cross polarization/magic angle	274:309	(13)C cross polarization/magic angle spinning spectra	274:326	(13)C cross polarization/magic angle spinning spectra were recorded from BZL and from BZL:β-CD, BZL:methyl β-CD and BZL:hydroxypropyl β-CD complexes, which were obtained by the solvent evaporation technique.
26256164	4	53	theme	BZL	731:733	arg1	complexes					757:765	stable BZL:methyl β-CD inclusion complexes	724:765	stable BZL:methyl β-CD inclusion complexes	724:765	Conversely, BZL:methyl β-CD displayed BZL resonances characteristic of an amorphous drug and data analysis confirmed the presence of stable BZL:methyl β-CD inclusion complexes, with benzene encapsulated into the host cavity.
26256164	4	54	theme	stable	724:729	arg1	complexes					757:765	stable BZL:methyl β-CD inclusion complexes	724:765	stable BZL:methyl β-CD inclusion complexes	724:765	Conversely, BZL:methyl β-CD displayed BZL resonances characteristic of an amorphous drug and data analysis confirmed the presence of stable BZL:methyl β-CD inclusion complexes, with benzene encapsulated into the host cavity.
26256164	4	55	theme	methyl	735:740	arg1	complexes					757:765	stable BZL:methyl β-CD inclusion complexes	724:765	stable BZL:methyl β-CD inclusion complexes	724:765	Conversely, BZL:methyl β-CD displayed BZL resonances characteristic of an amorphous drug and data analysis confirmed the presence of stable BZL:methyl β-CD inclusion complexes, with benzene encapsulated into the host cavity.
26256164	2	56	theme	cross	280:284	arg1	angle					305:309	(13)C cross polarization/magic angle	274:309	(13)C cross polarization/magic angle spinning spectra	274:326	(13)C cross polarization/magic angle spinning spectra were recorded from BZL and from BZL:β-CD, BZL:methyl β-CD and BZL:hydroxypropyl β-CD complexes, which were obtained by the solvent evaporation technique.
26256164	4	57	with	complexes	757:765	arg1	benzene					773:779	benzene	773:779	benzene encapsulated into the host cavity	773:813	Conversely, BZL:methyl β-CD displayed BZL resonances characteristic of an amorphous drug and data analysis confirmed the presence of stable BZL:methyl β-CD inclusion complexes, with benzene encapsulated into the host cavity.
26256164	5	58	theme	relaxation	918:927	arg1	times					929:933	proton and carbon spin-lattice relaxation times	887:933	times	929:933	Further evidences on complex structure and dynamics were obtained from proton and carbon spin-lattice relaxation times in the rotating frame.
26256164	2	59	theme	C	278:278	arg1	angle					305:309	(13)C cross polarization/magic angle	274:309	(13)C cross polarization/magic angle spinning spectra	274:326	(13)C cross polarization/magic angle spinning spectra were recorded from BZL and from BZL:β-CD, BZL:methyl β-CD and BZL:hydroxypropyl β-CD complexes, which were obtained by the solvent evaporation technique.
26256164	0	60	with	complexes	49:57	arg1	derivatives					93:103	cyclodextrin derivatives	80:103	cyclodextrin derivatives	80:103	First solid-state NMR spectroscopy evaluation of complexes of benznidazole with cyclodextrin derivatives.
26256164	5	61	theme	proton	887:892	arg1	times					929:933	proton and carbon spin-lattice relaxation times	887:933	times	929:933	Further evidences on complex structure and dynamics were obtained from proton and carbon spin-lattice relaxation times in the rotating frame.
26256164	1	62	theme	cyclodextrin	211:222	arg1	derivatives					229:239	cyclodextrin (CD) derivatives	211:239	cyclodextrin (CD) derivatives	211:239	Complexation of benznidazole (BZL), a drug of choice for the treatment of Chagas'neglected disease, with cyclodextrin (CD) derivatives was analyzed by solid-state NMR.
26256164	0	63	theme	benznidazole	62:73	arg1	complexes					49:57	complexes	49:57	complexes of benznidazole with cyclodextrin derivatives	49:103	First solid-state NMR spectroscopy evaluation of complexes of benznidazole with cyclodextrin derivatives.
26256164	0	64	theme	NMR	18:20	arg1	spectroscopy					22:33	solid-state NMR spectroscopy	6:33	First solid-state NMR spectroscopy evaluation of complexes of benznidazole with cyclodextrin derivatives.	0:104	First solid-state NMR spectroscopy evaluation of complexes of benznidazole with cyclodextrin derivatives.
26256164	4	65	theme	methyl	607:612	arg1	β-CD					614:617	BZL:methyl β-CD	603:617	BZL:methyl β-CD	603:617	Conversely, BZL:methyl β-CD displayed BZL resonances characteristic of an amorphous drug and data analysis confirmed the presence of stable BZL:methyl β-CD inclusion complexes, with benzene encapsulated into the host cavity.
26256164	8	66	with	mixtures	1116:1123	arg1	BZL					1140:1142	identical BZL	1130:1142	identical BZL	1130:1142	Physical mixtures with identical BZL and CD compositions were also studied for comparison.
26256164	8	66	with	mixtures	1116:1123	arg1	compositions					1151:1162	CD compositions	1148:1162	CD compositions	1148:1162	Physical mixtures with identical BZL and CD compositions were also studied for comparison.
26935325	0	0	theme	denitrification	95:109	arg1	process					111:117	denitrification process	95:117	denitrification process	95:117	Greenhouse gas emission and microbial community dynamics during simultaneous nitrification and denitrification process.
26935325	5	1	theme	polymeric	540:548	arg1	EPS					561:563	EPS	561:563	EPS	561:563	Extracellular polymeric substance (EPS) contents in activated sludge increased during start-up the SND process.
26935325	5	1	theme	polymeric	540:548	arg1	substance					550:558	Extracellular polymeric substance	526:558	Extracellular polymeric substance (EPS) contents in activated sludge	526:593	Extracellular polymeric substance (EPS) contents in activated sludge increased during start-up the SND process.
26935325	1	2	theme	microbial	173:181	arg1	dynamics					193:200	the microbial community dynamics	169:200	the microbial community dynamics during simultaneous nitrification and denitrification (SND) process	169:268	This study evaluates greenhouse gas emission and the microbial community dynamics during simultaneous nitrification and denitrification (SND) process.
26935325	1	3	theme	community	183:191	arg1	dynamics					193:200	the microbial community dynamics	169:200	the microbial community dynamics during simultaneous nitrification and denitrification (SND) process	169:268	This study evaluates greenhouse gas emission and the microbial community dynamics during simultaneous nitrification and denitrification (SND) process.
26935325	2	4	theme	released	313:320	arg1	4.28g					322:326	the SND reactor released 4.28g	297:326	the SND reactor released 4.28g of greenhouse	297:340	Based on CO2 equivalents, the SND reactor released 4.28g of greenhouse gases each cycle.
26935325	6	5	theme	EPS	735:737	arg1	content					739:745	EPS content	735:745	EPS content	735:745	High-throughput sequencing showed increases in bacterial species richness, leading to changes in EPS content and composition observed using 3D-EEM fluorescence spectra.
26935325	3	6	theme	incoming	373:380	arg1	load					391:394	the incoming nitrogen load	369:394	the incoming nitrogen load	369:394	2.91% of the incoming nitrogen load was emitted as N2O.
26935325	8	7	theme	phosphorus-accumulating	966:988	arg1	bacteria					990:997	phosphorus-accumulating bacteria	966:997	phosphorus-accumulating bacteria	966:997	For phosphorus-accumulating bacteria, the relative abundance of Rhodocyclaceae also significantly increased.
26935325	9	8	theme	SND	1265:1267	arg1	process					1269:1275	the SND process	1261:1275	the SND process	1261:1275	The relative abundance of other functional microbes, such as Nitrosomonadaceae (ammonia oxidizer), Nitrospirales (nitrite oxidizer) and Planctomyces (anammox) decreased significantly during the SND process.
26935325	2	9	theme	reactor	305:311	arg1	4.28g					322:326	the SND reactor released 4.28g	297:326	the SND reactor released 4.28g of greenhouse	297:340	Based on CO2 equivalents, the SND reactor released 4.28g of greenhouse gases each cycle.
26935325	9	10	theme	ammonia	1151:1157	arg1	Nitrosomonadaceae					1132:1148	Nitrosomonadaceae	1132:1148	Nitrosomonadaceae (ammonia oxidizer)	1132:1167	The relative abundance of other functional microbes, such as Nitrosomonadaceae (ammonia oxidizer), Nitrospirales (nitrite oxidizer) and Planctomyces (anammox) decreased significantly during the SND process.
26935325	9	10	theme	ammonia	1151:1157	arg1	oxidizer					1159:1166	ammonia oxidizer	1151:1166	ammonia oxidizer	1151:1166	The relative abundance of other functional microbes, such as Nitrosomonadaceae (ammonia oxidizer), Nitrospirales (nitrite oxidizer) and Planctomyces (anammox) decreased significantly during the SND process.
26935325	9	11	theme	nitrite	1185:1191	arg1	Nitrospirales					1170:1182	Nitrospirales	1170:1182	Nitrospirales (nitrite oxidizer)	1170:1201	The relative abundance of other functional microbes, such as Nitrosomonadaceae (ammonia oxidizer), Nitrospirales (nitrite oxidizer) and Planctomyces (anammox) decreased significantly during the SND process.
26935325	9	11	theme	nitrite	1185:1191	arg1	oxidizer					1193:1200	nitrite oxidizer	1185:1200	nitrite oxidizer	1185:1200	The relative abundance of other functional microbes, such as Nitrosomonadaceae (ammonia oxidizer), Nitrospirales (nitrite oxidizer) and Planctomyces (anammox) decreased significantly during the SND process.
26935325	4	12	theme	aerobic	465:471	arg1	stage					473:477	the aerobic stage	461:477	the aerobic stage	461:477	The CO2 and N2O emissions mainly occurred in the aerobic stage and CH4 emissions were consistently near zero.
26935325	5	13	theme	SND	625:627	arg1	process					629:635	the SND process	621:635	start-up the SND process	612:635	Extracellular polymeric substance (EPS) contents in activated sludge increased during start-up the SND process.
26935325	2	14	theme	SND	301:303	arg1	4.28g					322:326	the SND reactor released 4.28g	297:326	the SND reactor released 4.28g of greenhouse	297:340	Based on CO2 equivalents, the SND reactor released 4.28g of greenhouse gases each cycle.
26935325	7	15	theme	SND	907:909	arg1	process					911:917	the SND process	903:917	the SND process	903:917	For denitrifying bacteria, the relative abundance of Pseudomonas significantly increased during the SND process, while Paracoccus decreased significantly.
26935325	6	16	from	changes	724:730	arg1	composition					751:761	composition	751:761	composition	751:761	High-throughput sequencing showed increases in bacterial species richness, leading to changes in EPS content and composition observed using 3D-EEM fluorescence spectra.
26935325	6	16	from	changes	724:730	arg1	content					739:745	EPS content	735:745	EPS content	735:745	High-throughput sequencing showed increases in bacterial species richness, leading to changes in EPS content and composition observed using 3D-EEM fluorescence spectra.
26935325	0	17	theme	gas	11:13	arg1	emission					15:22	Greenhouse gas emission	0:22	Greenhouse gas emission	0:22	Greenhouse gas emission and microbial community dynamics during simultaneous nitrification and denitrification process.
26935325	9	18	theme	other	1097:1101	arg1	microbes					1114:1121	other functional microbes	1097:1121	other functional microbes	1097:1121	The relative abundance of other functional microbes, such as Nitrosomonadaceae (ammonia oxidizer), Nitrospirales (nitrite oxidizer) and Planctomyces (anammox) decreased significantly during the SND process.
26935325	9	18	theme	other	1097:1101	arg1	Planctomyces					1207:1218	Planctomyces	1207:1218	Planctomyces (anammox)	1207:1228	The relative abundance of other functional microbes, such as Nitrosomonadaceae (ammonia oxidizer), Nitrospirales (nitrite oxidizer) and Planctomyces (anammox) decreased significantly during the SND process.
26935325	9	18	theme	other	1097:1101	arg1	Nitrosomonadaceae					1132:1148	Nitrosomonadaceae	1132:1148	Nitrosomonadaceae (ammonia oxidizer)	1132:1167	The relative abundance of other functional microbes, such as Nitrosomonadaceae (ammonia oxidizer), Nitrospirales (nitrite oxidizer) and Planctomyces (anammox) decreased significantly during the SND process.
26935325	9	18	theme	other	1097:1101	arg1	Nitrospirales					1170:1182	Nitrospirales	1170:1182	Nitrospirales (nitrite oxidizer)	1170:1201	The relative abundance of other functional microbes, such as Nitrosomonadaceae (ammonia oxidizer), Nitrospirales (nitrite oxidizer) and Planctomyces (anammox) decreased significantly during the SND process.
26935325	1	19	theme	simultaneous	209:220	arg1	process					262:268	simultaneous nitrification and denitrification (SND) process	209:268	simultaneous nitrification and denitrification (SND) process	209:268	This study evaluates greenhouse gas emission and the microbial community dynamics during simultaneous nitrification and denitrification (SND) process.
26935325	0	20	theme	Greenhouse	0:9	arg1	emission					15:22	Greenhouse gas emission	0:22	Greenhouse gas emission	0:22	Greenhouse gas emission and microbial community dynamics during simultaneous nitrification and denitrification process.
26935325	5	21	theme	activated	578:586	arg1	sludge					588:593	activated sludge	578:593	activated sludge	578:593	Extracellular polymeric substance (EPS) contents in activated sludge increased during start-up the SND process.
26935325	2	22	theme	greenhouse	331:340	arg1	4.28g					322:326	the SND reactor released 4.28g	297:326	the SND reactor released 4.28g of greenhouse	297:340	Based on CO2 equivalents, the SND reactor released 4.28g of greenhouse gases each cycle.
26935325	7	23	theme	Pseudomonas	860:870	arg1	abundance					847:855	the relative abundance	834:855	the relative abundance of Pseudomonas	834:870	For denitrifying bacteria, the relative abundance of Pseudomonas significantly increased during the SND process, while Paracoccus decreased significantly.
26935325	1	24	theme	nitrification	222:234	arg1	process					262:268	simultaneous nitrification and denitrification (SND) process	209:268	simultaneous nitrification and denitrification (SND) process	209:268	This study evaluates greenhouse gas emission and the microbial community dynamics during simultaneous nitrification and denitrification (SND) process.
26935325	3	25	theme	nitrogen	382:389	arg1	load					391:394	the incoming nitrogen load	369:394	the incoming nitrogen load	369:394	2.91% of the incoming nitrogen load was emitted as N2O.
26935325	7	26	theme	relative	838:845	arg1	abundance					847:855	the relative abundance	834:855	the relative abundance of Pseudomonas	834:870	For denitrifying bacteria, the relative abundance of Pseudomonas significantly increased during the SND process, while Paracoccus decreased significantly.
26935325	3	27	theme	load	391:394	arg1	N2O					411:413	N2O	411:413	N2O	411:413	2.91% of the incoming nitrogen load was emitted as N2O.
26935325	3	27	theme	load	391:394	arg1	load					391:394	the incoming nitrogen load	369:394	the incoming nitrogen load	369:394	2.91% of the incoming nitrogen load was emitted as N2O.
26935325	3	27	theme	load	391:394	arg1	%					364:364	2.91%	360:364	2.91% of the incoming nitrogen load	360:394	2.91% of the incoming nitrogen load was emitted as N2O.
26935325	4	28	dep	stage	473:477	arg1	emissions					487:495	emissions	487:495	emissions	487:495	The CO2 and N2O emissions mainly occurred in the aerobic stage and CH4 emissions were consistently near zero.
26935325	6	29	theme	species	695:701	arg1	richness					703:710	bacterial species richness	685:710	bacterial species richness	685:710	High-throughput sequencing showed increases in bacterial species richness, leading to changes in EPS content and composition observed using 3D-EEM fluorescence spectra.
26935325	1	30	theme	denitrification	240:254	arg1	process					262:268	simultaneous nitrification and denitrification (SND) process	209:268	simultaneous nitrification and denitrification (SND) process	209:268	This study evaluates greenhouse gas emission and the microbial community dynamics during simultaneous nitrification and denitrification (SND) process.
26935325	0	31	theme	community	38:46	arg1	dynamics					48:55	microbial community dynamics	28:55	microbial community dynamics	28:55	Greenhouse gas emission and microbial community dynamics during simultaneous nitrification and denitrification process.
26935325	6	32	theme	bacterial	685:693	arg1	species					695:701	bacterial species	685:701	bacterial species richness	685:710	High-throughput sequencing showed increases in bacterial species richness, leading to changes in EPS content and composition observed using 3D-EEM fluorescence spectra.
26935325	0	33	theme	microbial	28:36	arg1	dynamics					48:55	microbial community dynamics	28:55	microbial community dynamics	28:55	Greenhouse gas emission and microbial community dynamics during simultaneous nitrification and denitrification process.
26935325	9	34	theme	relative	1075:1082	arg1	abundance					1084:1092	The relative abundance	1071:1092	The relative abundance of other functional microbes, such as Nitrosomonadaceae (ammonia oxidizer), Nitrospirales (nitrite oxidizer) and Planctomyces (anammox)	1071:1228	The relative abundance of other functional microbes, such as Nitrosomonadaceae (ammonia oxidizer), Nitrospirales (nitrite oxidizer) and Planctomyces (anammox) decreased significantly during the SND process.
26935325	5	35	theme	substance	550:558	arg1	contents					566:573	Extracellular polymeric substance (EPS) contents	526:573	Extracellular polymeric substance (EPS) contents in activated sludge	526:593	Extracellular polymeric substance (EPS) contents in activated sludge increased during start-up the SND process.
26935325	7	36	theme	denitrifying	811:822	arg1	bacteria					824:831	denitrifying bacteria	811:831	denitrifying bacteria	811:831	For denitrifying bacteria, the relative abundance of Pseudomonas significantly increased during the SND process, while Paracoccus decreased significantly.
26935325	6	37	theme	3D-EEM	778:783	arg1	spectra					798:804	3D-EEM fluorescence spectra	778:804	3D-EEM fluorescence spectra	778:804	High-throughput sequencing showed increases in bacterial species richness, leading to changes in EPS content and composition observed using 3D-EEM fluorescence spectra.
26935325	6	38	from	increases	672:680	arg1	richness					703:710	bacterial species richness	685:710	bacterial species richness	685:710	High-throughput sequencing showed increases in bacterial species richness, leading to changes in EPS content and composition observed using 3D-EEM fluorescence spectra.
26935325	1	39	theme	greenhouse	141:150	arg1	emission					156:163	greenhouse gas emission	141:163	greenhouse gas emission	141:163	This study evaluates greenhouse gas emission and the microbial community dynamics during simultaneous nitrification and denitrification (SND) process.
26935325	5	40	dep	start-up	612:619	arg1	process					629:635	the SND process	621:635	start-up the SND process	612:635	Extracellular polymeric substance (EPS) contents in activated sludge increased during start-up the SND process.
26935325	4	41	theme	CO2	420:422	arg1	emissions					432:440	The CO2 and N2O emissions	416:440	The CO2 and N2O emissions mainly occurred in the aerobic stage and CH4 emissions	416:495	The CO2 and N2O emissions mainly occurred in the aerobic stage and CH4 emissions were consistently near zero.
26935325	1	42	theme	gas	152:154	arg1	emission					156:163	greenhouse gas emission	141:163	greenhouse gas emission	141:163	This study evaluates greenhouse gas emission and the microbial community dynamics during simultaneous nitrification and denitrification (SND) process.
26935325	8	43	theme	Rhodocyclaceae	1026:1039	arg1	abundance					1013:1021	the relative abundance	1000:1021	the relative abundance of Rhodocyclaceae	1000:1039	For phosphorus-accumulating bacteria, the relative abundance of Rhodocyclaceae also significantly increased.
26935325	6	44	theme	fluorescence	785:796	arg1	spectra					798:804	3D-EEM fluorescence spectra	778:804	3D-EEM fluorescence spectra	778:804	High-throughput sequencing showed increases in bacterial species richness, leading to changes in EPS content and composition observed using 3D-EEM fluorescence spectra.
26935325	9	45	theme	microbes	1114:1121	arg1	abundance					1084:1092	The relative abundance	1071:1092	The relative abundance of other functional microbes, such as Nitrosomonadaceae (ammonia oxidizer), Nitrospirales (nitrite oxidizer) and Planctomyces (anammox)	1071:1228	The relative abundance of other functional microbes, such as Nitrosomonadaceae (ammonia oxidizer), Nitrospirales (nitrite oxidizer) and Planctomyces (anammox) decreased significantly during the SND process.
26935325	5	46	from	contents	566:573	arg1	sludge					588:593	activated sludge	578:593	activated sludge	578:593	Extracellular polymeric substance (EPS) contents in activated sludge increased during start-up the SND process.
26935325	4	47	theme	N2O	428:430	arg1	emissions					432:440	The CO2 and N2O emissions	416:440	The CO2 and N2O emissions mainly occurred in the aerobic stage and CH4 emissions	416:495	The CO2 and N2O emissions mainly occurred in the aerobic stage and CH4 emissions were consistently near zero.
26935325	2	48	theme	CO2	280:282	arg1	equivalents					284:294	CO2 equivalents	280:294	CO2 equivalents	280:294	Based on CO2 equivalents, the SND reactor released 4.28g of greenhouse gases each cycle.
26935325	8	49	theme	relative	1004:1011	arg1	abundance					1013:1021	the relative abundance	1000:1021	the relative abundance of Rhodocyclaceae	1000:1039	For phosphorus-accumulating bacteria, the relative abundance of Rhodocyclaceae also significantly increased.
26935325	9	50	theme	functional	1103:1112	arg1	microbes					1114:1121	other functional microbes	1097:1121	other functional microbes	1097:1121	The relative abundance of other functional microbes, such as Nitrosomonadaceae (ammonia oxidizer), Nitrospirales (nitrite oxidizer) and Planctomyces (anammox) decreased significantly during the SND process.
26935325	9	50	theme	functional	1103:1112	arg1	Planctomyces					1207:1218	Planctomyces	1207:1218	Planctomyces (anammox)	1207:1228	The relative abundance of other functional microbes, such as Nitrosomonadaceae (ammonia oxidizer), Nitrospirales (nitrite oxidizer) and Planctomyces (anammox) decreased significantly during the SND process.
26935325	9	50	theme	functional	1103:1112	arg1	Nitrosomonadaceae					1132:1148	Nitrosomonadaceae	1132:1148	Nitrosomonadaceae (ammonia oxidizer)	1132:1167	The relative abundance of other functional microbes, such as Nitrosomonadaceae (ammonia oxidizer), Nitrospirales (nitrite oxidizer) and Planctomyces (anammox) decreased significantly during the SND process.
26935325	9	50	theme	functional	1103:1112	arg1	Nitrospirales					1170:1182	Nitrospirales	1170:1182	Nitrospirales (nitrite oxidizer)	1170:1201	The relative abundance of other functional microbes, such as Nitrosomonadaceae (ammonia oxidizer), Nitrospirales (nitrite oxidizer) and Planctomyces (anammox) decreased significantly during the SND process.
26935325	0	51	theme	simultaneous	64:75	arg1	nitrification					77:89	simultaneous nitrification	64:89	simultaneous nitrification	64:89	Greenhouse gas emission and microbial community dynamics during simultaneous nitrification and denitrification process.
26935325	5	52	theme	Extracellular	526:538	arg1	EPS					561:563	EPS	561:563	EPS	561:563	Extracellular polymeric substance (EPS) contents in activated sludge increased during start-up the SND process.
26935325	5	52	theme	Extracellular	526:538	arg1	substance					550:558	Extracellular polymeric substance	526:558	Extracellular polymeric substance (EPS) contents in activated sludge	526:593	Extracellular polymeric substance (EPS) contents in activated sludge increased during start-up the SND process.
26935325	6	53	theme	High-throughput	638:652	arg1	sequencing					654:663	High-throughput sequencing	638:663	High-throughput sequencing	638:663	High-throughput sequencing showed increases in bacterial species richness, leading to changes in EPS content and composition observed using 3D-EEM fluorescence spectra.
28425501	0	0	from	identification	10:23	arg1	artworks					37:44	historic artworks	28:44	historic artworks	28:44	Plant gum identification in historic artworks.
28425501	5	1	theme	locust	683:688	arg1	gums					695:698	cherry and locust bean gums	672:698	gums	695:698	A wide range of oligosaccharidic combinations of pentose, hexose, deoxyhexose and hexuronic acid were accurately identified in gum arabic whereas cherry and locust bean gums showed respectively PentxHexy and Hexn profiles.
28425501	5	2	theme	pentose	575:581	arg1	combinations					559:570	oligosaccharidic combinations	542:570	oligosaccharidic combinations of pentose, hexose, deoxyhexose and hexuronic acid	542:621	A wide range of oligosaccharidic combinations of pentose, hexose, deoxyhexose and hexuronic acid were accurately identified in gum arabic whereas cherry and locust bean gums showed respectively PentxHexy and Hexn profiles.
28425501	1	3	theme	sample	157:162	arg1	heritage					191:198	cultural heritage	182:198	cultural heritage	182:198	We describe an integrated and straightforward new analytical protocol that identifies plant gums from various sample sources including cultural heritage.
28425501	1	3	theme	sample	157:162	arg1	sources					164:170	various sample sources	149:170	various sample sources including cultural heritage	149:198	We describe an integrated and straightforward new analytical protocol that identifies plant gums from various sample sources including cultural heritage.
28425501	7	4	theme	cultural	1057:1064	arg1	sample					1075:1080	a cultural heritage sample	1055:1080	a cultural heritage sample using structural information	1055:1109	This is the first time that a gum is accurately identified in a cultural heritage sample using structural information.
28425501	3	5	theme	enzyme	356:361	arg1	cocktail					363:370	an enzyme cocktail	353:370	an enzyme cocktail suitable for plant gums of unknown composition	353:417	We developed an enzyme cocktail suitable for plant gums of unknown composition.
28425501	5	6	theme	bean	690:693	arg1	gums					695:698	cherry and locust bean gums	672:698	gums	695:698	A wide range of oligosaccharidic combinations of pentose, hexose, deoxyhexose and hexuronic acid were accurately identified in gum arabic whereas cherry and locust bean gums showed respectively PentxHexy and Hexn profiles.
28425501	6	7	theme	Roberson	902:909	arg1	&Co					911:913	Colour Box Charles Roberson &Co	883:913	'Colour Box Charles Roberson &Co' dating 1870s and drawings from the American painter Arthur Dove (1880-1946)	882:990	Optimized for low sample quantities, the analytical protocol was successfully applied to contemporary and historic samples including 'Colour Box Charles Roberson &Co' dating 1870s and drawings from the American painter Arthur Dove (1880-1946).
28425501	1	8	from	sources	164:170	arg1	gums					139:142	plant gums	133:142	plant gums from various sample sources including cultural heritage	133:198	We describe an integrated and straightforward new analytical protocol that identifies plant gums from various sample sources including cultural heritage.
28425501	2	9	theme	enzymatic	318:326	arg1	hydrolysis					328:337	controlled enzymatic hydrolysis	307:337	controlled enzymatic hydrolysis	307:337	Our approach is based on the identification of saccharidic fingerprints using mass spectrometry following controlled enzymatic hydrolysis.
28425501	6	10	theme	low	763:765	arg1	quantities					774:783	low sample quantities	763:783	low sample quantities	763:783	Optimized for low sample quantities, the analytical protocol was successfully applied to contemporary and historic samples including 'Colour Box Charles Roberson &Co' dating 1870s and drawings from the American painter Arthur Dove (1880-1946).
28425501	4	11	theme	gums	447:450	arg1	profiles					435:442	Distinctive MS profiles	420:442	Distinctive MS profiles of gums such as arabic, cherry and locust-bean gums	420:494	Distinctive MS profiles of gums such as arabic, cherry and locust-bean gums were successfully identified.
28425501	2	12	theme	controlled	307:316	arg1	hydrolysis					328:337	controlled enzymatic hydrolysis	307:337	controlled enzymatic hydrolysis	307:337	Our approach is based on the identification of saccharidic fingerprints using mass spectrometry following controlled enzymatic hydrolysis.
28425501	8	13	theme	other	1159:1163	arg1	domains					1165:1171	other domains	1159:1171	other domains (food, cosmetic, pharmaceutical, biomedical)	1159:1216	Furthermore, this methodology is applicable to other domains (food, cosmetic, pharmaceutical, biomedical).
28425501	8	14	dep	food	1174:1177	arg1	cosmetic					1180:1187	cosmetic	1180:1187	cosmetic	1180:1187	Furthermore, this methodology is applicable to other domains (food, cosmetic, pharmaceutical, biomedical).
28425501	8	14	dep	food	1174:1177	arg1	biomedical					1206:1215	biomedical	1206:1215	biomedical	1206:1215	Furthermore, this methodology is applicable to other domains (food, cosmetic, pharmaceutical, biomedical).
28425501	8	14	dep	food	1174:1177	arg1	pharmaceutical					1190:1203	pharmaceutical	1190:1203	pharmaceutical	1190:1203	Furthermore, this methodology is applicable to other domains (food, cosmetic, pharmaceutical, biomedical).
28425501	5	15	theme	gum	653:655	arg1	arabic					657:662	gum arabic	653:662	gum arabic	653:662	A wide range of oligosaccharidic combinations of pentose, hexose, deoxyhexose and hexuronic acid were accurately identified in gum arabic whereas cherry and locust bean gums showed respectively PentxHexy and Hexn profiles.
28425501	1	16	theme	integrated	62:71	arg1	protocol					108:115	an integrated and straightforward new analytical protocol	59:115	an integrated and straightforward new analytical protocol that identifies plant gums from various sample sources including cultural heritage	59:198	We describe an integrated and straightforward new analytical protocol that identifies plant gums from various sample sources including cultural heritage.
28425501	5	17	theme	wide	528:531	arg1	range					533:537	A wide range	526:537	A wide range of oligosaccharidic combinations of pentose, hexose, deoxyhexose and hexuronic acid	526:621	A wide range of oligosaccharidic combinations of pentose, hexose, deoxyhexose and hexuronic acid were accurately identified in gum arabic whereas cherry and locust bean gums showed respectively PentxHexy and Hexn profiles.
28425501	3	18	theme	plant	385:389	arg1	gums					391:394	plant gums	385:394	plant gums of unknown composition	385:417	We developed an enzyme cocktail suitable for plant gums of unknown composition.
28425501	6	19	theme	contemporary	838:849	arg1	&Co					911:913	Colour Box Charles Roberson &Co	883:913	'Colour Box Charles Roberson &Co' dating 1870s and drawings from the American painter Arthur Dove (1880-1946)	882:990	Optimized for low sample quantities, the analytical protocol was successfully applied to contemporary and historic samples including 'Colour Box Charles Roberson &Co' dating 1870s and drawings from the American painter Arthur Dove (1880-1946).
28425501	6	19	theme	contemporary	838:849	arg1	samples					864:870	contemporary and historic samples	838:870	contemporary and historic samples including 'Colour Box Charles Roberson &Co' dating 1870s and drawings from the American painter Arthur Dove (1880-1946)	838:990	Optimized for low sample quantities, the analytical protocol was successfully applied to contemporary and historic samples including 'Colour Box Charles Roberson &Co' dating 1870s and drawings from the American painter Arthur Dove (1880-1946).
28425501	1	20	theme	cultural	182:189	arg1	heritage					191:198	cultural heritage	182:198	cultural heritage	182:198	We describe an integrated and straightforward new analytical protocol that identifies plant gums from various sample sources including cultural heritage.
28425501	0	21	theme	gum	6:8	arg1	identification					10:23	Plant gum identification	0:23	Plant gum identification in historic artworks.	0:45	Plant gum identification in historic artworks.
28425501	5	22	theme	cherry	672:677	arg1	gums					695:698	cherry and locust bean gums	672:698	gums	695:698	A wide range of oligosaccharidic combinations of pentose, hexose, deoxyhexose and hexuronic acid were accurately identified in gum arabic whereas cherry and locust bean gums showed respectively PentxHexy and Hexn profiles.
28425501	5	23	theme	hexuronic	608:616	arg1	acid					618:621	hexuronic acid	608:621	hexuronic acid	608:621	A wide range of oligosaccharidic combinations of pentose, hexose, deoxyhexose and hexuronic acid were accurately identified in gum arabic whereas cherry and locust bean gums showed respectively PentxHexy and Hexn profiles.
28425501	1	24	theme	straightforward	77:91	arg1	protocol					108:115	an integrated and straightforward new analytical protocol	59:115	an integrated and straightforward new analytical protocol that identifies plant gums from various sample sources including cultural heritage	59:198	We describe an integrated and straightforward new analytical protocol that identifies plant gums from various sample sources including cultural heritage.
28425501	0	25	theme	Plant	0:4	arg1	identification					10:23	Plant gum identification	0:23	Plant gum identification in historic artworks.	0:45	Plant gum identification in historic artworks.
28425501	4	26	theme	Distinctive	420:430	arg1	profiles					435:442	Distinctive MS profiles	420:442	Distinctive MS profiles of gums such as arabic, cherry and locust-bean gums	420:494	Distinctive MS profiles of gums such as arabic, cherry and locust-bean gums were successfully identified.
28425501	1	27	theme	new	93:95	arg1	protocol					108:115	an integrated and straightforward new analytical protocol	59:115	an integrated and straightforward new analytical protocol that identifies plant gums from various sample sources including cultural heritage	59:198	We describe an integrated and straightforward new analytical protocol that identifies plant gums from various sample sources including cultural heritage.
28425501	3	28	theme	unknown	399:405	arg1	composition					407:417	unknown composition	399:417	unknown composition	399:417	We developed an enzyme cocktail suitable for plant gums of unknown composition.
28425501	5	29	theme	acid	618:621	arg1	combinations					559:570	oligosaccharidic combinations	542:570	oligosaccharidic combinations of pentose, hexose, deoxyhexose and hexuronic acid	542:621	A wide range of oligosaccharidic combinations of pentose, hexose, deoxyhexose and hexuronic acid were accurately identified in gum arabic whereas cherry and locust bean gums showed respectively PentxHexy and Hexn profiles.
28425501	7	30	theme	structural	1088:1097	arg1	information					1099:1109	structural information	1088:1109	structural information	1088:1109	This is the first time that a gum is accurately identified in a cultural heritage sample using structural information.
28425501	1	31	theme	analytical	97:106	arg1	protocol					108:115	an integrated and straightforward new analytical protocol	59:115	an integrated and straightforward new analytical protocol that identifies plant gums from various sample sources including cultural heritage	59:198	We describe an integrated and straightforward new analytical protocol that identifies plant gums from various sample sources including cultural heritage.
28425501	7	32	dep	time	1011:1014	arg1	identified					1041:1050	identified	1041:1050	is accurately identified in a cultural heritage sample using structural information	1027:1109	This is the first time that a gum is accurately identified in a cultural heritage sample using structural information.
28425501	3	33	theme	composition	407:417	arg1	gums					391:394	plant gums	385:394	plant gums of unknown composition	385:417	We developed an enzyme cocktail suitable for plant gums of unknown composition.
28425501	0	34	theme	historic	28:35	arg1	artworks					37:44	historic artworks	28:44	historic artworks	28:44	Plant gum identification in historic artworks.
28425501	6	35	theme	historic	855:862	arg1	&Co					911:913	Colour Box Charles Roberson &Co	883:913	'Colour Box Charles Roberson &Co' dating 1870s and drawings from the American painter Arthur Dove (1880-1946)	882:990	Optimized for low sample quantities, the analytical protocol was successfully applied to contemporary and historic samples including 'Colour Box Charles Roberson &Co' dating 1870s and drawings from the American painter Arthur Dove (1880-1946).
28425501	6	35	theme	historic	855:862	arg1	samples					864:870	contemporary and historic samples	838:870	contemporary and historic samples including 'Colour Box Charles Roberson &Co' dating 1870s and drawings from the American painter Arthur Dove (1880-1946)	838:990	Optimized for low sample quantities, the analytical protocol was successfully applied to contemporary and historic samples including 'Colour Box Charles Roberson &Co' dating 1870s and drawings from the American painter Arthur Dove (1880-1946).
28425501	4	36	theme	locust-bean	479:489	arg1	gums					491:494	locust-bean gums	479:494	locust-bean gums	479:494	Distinctive MS profiles of gums such as arabic, cherry and locust-bean gums were successfully identified.
28425501	5	37	theme	hexose	584:589	arg1	combinations					559:570	oligosaccharidic combinations	542:570	oligosaccharidic combinations of pentose, hexose, deoxyhexose and hexuronic acid	542:621	A wide range of oligosaccharidic combinations of pentose, hexose, deoxyhexose and hexuronic acid were accurately identified in gum arabic whereas cherry and locust bean gums showed respectively PentxHexy and Hexn profiles.
28425501	7	38	theme	heritage	1066:1073	arg1	sample					1075:1080	a cultural heritage sample	1055:1080	a cultural heritage sample using structural information	1055:1109	This is the first time that a gum is accurately identified in a cultural heritage sample using structural information.
28425501	6	39	theme	Charles	894:900	arg1	&Co					911:913	Colour Box Charles Roberson &Co	883:913	'Colour Box Charles Roberson &Co' dating 1870s and drawings from the American painter Arthur Dove (1880-1946)	882:990	Optimized for low sample quantities, the analytical protocol was successfully applied to contemporary and historic samples including 'Colour Box Charles Roberson &Co' dating 1870s and drawings from the American painter Arthur Dove (1880-1946).
28425501	8	40	dep	domains	1165:1171	arg1	food					1174:1177	food	1174:1177	food	1174:1177	Furthermore, this methodology is applicable to other domains (food, cosmetic, pharmaceutical, biomedical).
28425501	6	41	theme	analytical	790:799	arg1	protocol					801:808	the analytical protocol	786:808	the analytical protocol	786:808	Optimized for low sample quantities, the analytical protocol was successfully applied to contemporary and historic samples including 'Colour Box Charles Roberson &Co' dating 1870s and drawings from the American painter Arthur Dove (1880-1946).
28425501	5	42	theme	deoxyhexose	592:602	arg1	combinations					559:570	oligosaccharidic combinations	542:570	oligosaccharidic combinations of pentose, hexose, deoxyhexose and hexuronic acid	542:621	A wide range of oligosaccharidic combinations of pentose, hexose, deoxyhexose and hexuronic acid were accurately identified in gum arabic whereas cherry and locust bean gums showed respectively PentxHexy and Hexn profiles.
28425501	6	43	theme	Box	890:892	arg1	&Co					911:913	Colour Box Charles Roberson &Co	883:913	'Colour Box Charles Roberson &Co' dating 1870s and drawings from the American painter Arthur Dove (1880-1946)	882:990	Optimized for low sample quantities, the analytical protocol was successfully applied to contemporary and historic samples including 'Colour Box Charles Roberson &Co' dating 1870s and drawings from the American painter Arthur Dove (1880-1946).
28425501	2	44	theme	mass	279:282	arg1	spectrometry					284:295	mass spectrometry	279:295	mass spectrometry	279:295	Our approach is based on the identification of saccharidic fingerprints using mass spectrometry following controlled enzymatic hydrolysis.
28425501	4	45	theme	MS	432:433	arg1	profiles					435:442	Distinctive MS profiles	420:442	Distinctive MS profiles of gums such as arabic, cherry and locust-bean gums	420:494	Distinctive MS profiles of gums such as arabic, cherry and locust-bean gums were successfully identified.
28425501	1	46	theme	plant	133:137	arg1	gums					139:142	plant gums	133:142	plant gums from various sample sources including cultural heritage	133:198	We describe an integrated and straightforward new analytical protocol that identifies plant gums from various sample sources including cultural heritage.
28425501	7	47	theme	first	1005:1009	arg1	This					993:996	This	993:996	This	993:996	This is the first time that a gum is accurately identified in a cultural heritage sample using structural information.
28425501	7	47	theme	first	1005:1009	arg1	time					1011:1014	the first time	1001:1014	the first time that a gum is accurately identified in a cultural heritage sample using structural information	1001:1109	This is the first time that a gum is accurately identified in a cultural heritage sample using structural information.
28425501	5	48	theme	Hexn	734:737	arg1	profiles					739:746	Hexn profiles	734:746	Hexn profiles	734:746	A wide range of oligosaccharidic combinations of pentose, hexose, deoxyhexose and hexuronic acid were accurately identified in gum arabic whereas cherry and locust bean gums showed respectively PentxHexy and Hexn profiles.
28425501	6	49	theme	Colour	883:888	arg1	&Co					911:913	Colour Box Charles Roberson &Co	883:913	'Colour Box Charles Roberson &Co' dating 1870s and drawings from the American painter Arthur Dove (1880-1946)	882:990	Optimized for low sample quantities, the analytical protocol was successfully applied to contemporary and historic samples including 'Colour Box Charles Roberson &Co' dating 1870s and drawings from the American painter Arthur Dove (1880-1946).
28425501	5	50	theme	oligosaccharidic	542:557	arg1	combinations					559:570	oligosaccharidic combinations	542:570	oligosaccharidic combinations of pentose, hexose, deoxyhexose and hexuronic acid	542:621	A wide range of oligosaccharidic combinations of pentose, hexose, deoxyhexose and hexuronic acid were accurately identified in gum arabic whereas cherry and locust bean gums showed respectively PentxHexy and Hexn profiles.
28425501	2	51	theme	fingerprints	260:271	arg1	identification					230:243	the identification	226:243	the identification of saccharidic fingerprints using mass spectrometry following controlled enzymatic hydrolysis	226:337	Our approach is based on the identification of saccharidic fingerprints using mass spectrometry following controlled enzymatic hydrolysis.
28425501	5	52	theme	combinations	559:570	arg1	range					533:537	A wide range	526:537	A wide range of oligosaccharidic combinations of pentose, hexose, deoxyhexose and hexuronic acid	526:621	A wide range of oligosaccharidic combinations of pentose, hexose, deoxyhexose and hexuronic acid were accurately identified in gum arabic whereas cherry and locust bean gums showed respectively PentxHexy and Hexn profiles.
28425501	3	53	theme	suitable	372:379	arg1	cocktail					363:370	an enzyme cocktail	353:370	an enzyme cocktail suitable for plant gums of unknown composition	353:417	We developed an enzyme cocktail suitable for plant gums of unknown composition.
28425501	2	54	theme	saccharidic	248:258	arg1	fingerprints					260:271	saccharidic fingerprints	248:271	saccharidic fingerprints using mass spectrometry following controlled enzymatic hydrolysis	248:337	Our approach is based on the identification of saccharidic fingerprints using mass spectrometry following controlled enzymatic hydrolysis.
28425501	6	55	theme	sample	767:772	arg1	quantities					774:783	low sample quantities	763:783	low sample quantities	763:783	Optimized for low sample quantities, the analytical protocol was successfully applied to contemporary and historic samples including 'Colour Box Charles Roberson &Co' dating 1870s and drawings from the American painter Arthur Dove (1880-1946).
28425501	1	56	theme	various	149:155	arg1	heritage					191:198	cultural heritage	182:198	cultural heritage	182:198	We describe an integrated and straightforward new analytical protocol that identifies plant gums from various sample sources including cultural heritage.
28425501	1	56	theme	various	149:155	arg1	sources					164:170	various sample sources	149:170	various sample sources including cultural heritage	149:198	We describe an integrated and straightforward new analytical protocol that identifies plant gums from various sample sources including cultural heritage.
28039359	4	0	theme	mAG	631:633	arg1	composition					635:645	mAG composition	631:645	mAG composition	631:645	Elucidating microbe-specific differences in mAG composition could advance biotechnological applications and lead to new antimicrobial targets.
28039359	5	1	theme	UDP-galactopyranose	965:983	arg1	mutase					985:990	the enzyme UDP-galactopyranose mutase	954:990	the enzyme UDP-galactopyranose mutase (UGM or Glf)	954:1003	To this end, we compare and contrast galactan biosynthesis in C. diphtheriae and M. tuberculosis In each species, the galactan is constructed from uridine 5'-diphosphate-α-d-galactofuranose (UDP-Galf), which is generated by the enzyme UDP-galactopyranose mutase (UGM or Glf).
28039359	10	2	theme	M.	1718:1719	arg1	GlfT2					1734:1738	the M. tuberculosis GlfT2	1714:1738	the M. tuberculosis GlfT2	1714:1738	The C. diphtheriae GlfT2 gave rise to shorter polysaccharides than those obtained with the M. tuberculosis GlfT2.
28039359	7	3	theme	UGM	1166:1168	arg1	inhibitors					1152:1161	small molecule inhibitors	1137:1161	small molecule inhibitors of UGM	1137:1168	We show that small molecule inhibitors of UGM impede C. glutamicum growth, suggesting that the galactan is critical in corynebacteria.
28039359	7	4	theme	C.	1177:1178	arg1	growth					1191:1196	C. glutamicum growth	1177:1196	C. glutamicum growth	1177:1196	We show that small molecule inhibitors of UGM impede C. glutamicum growth, suggesting that the galactan is critical in corynebacteria.
28039359	2	5	theme	envelope	411:418	arg1	complex					457:463	the mycolyl-arabinogalactan (mAG) complex	423:463	the mycolyl-arabinogalactan (mAG) complex	423:463	A distinctive component of the Corynebacterineae cell envelope is the mycolyl-arabinogalactan (mAG) complex.
28039359	2	5	theme	envelope	411:418	arg1	component					371:379	A distinctive component	357:379	A distinctive component of the Corynebacterineae cell envelope	357:418	A distinctive component of the Corynebacterineae cell envelope is the mycolyl-arabinogalactan (mAG) complex.
28039359	12	6	dep	tools	1828:1832	arg1	genetic					1859:1865	genetic	1859:1865	genetic	1859:1865	Our results provide tools, both small molecule and genetic, for probing and perturbing the assembly of the Corynebacterineae cell envelope.
28039359	12	6	dep	tools	1828:1832	arg1	both					1835:1838	both	1835:1838	both	1835:1838	Our results provide tools, both small molecule and genetic, for probing and perturbing the assembly of the Corynebacterineae cell envelope.
28039359	9	7	theme	M.	1471:1472	arg1	polymerase					1500:1509	the M. tuberculosis carbohydrate polymerase	1467:1509	the M. tuberculosis carbohydrate polymerase responsible for the bulk of galactan polymerization, GlfT2,	1467:1569	To explore the source of galactan length variation, a C. diphtheriae ortholog of the M. tuberculosis carbohydrate polymerase responsible for the bulk of galactan polymerization, GlfT2, was produced, and its catalytic activity was evaluated.
28039359	9	8	dep	C.	1440:1441	arg1	diphtheriae					1443:1453	diphtheriae	1443:1453	diphtheriae	1443:1453	To explore the source of galactan length variation, a C. diphtheriae ortholog of the M. tuberculosis carbohydrate polymerase responsible for the bulk of galactan polymerization, GlfT2, was produced, and its catalytic activity was evaluated.
28039359	9	9	theme	catalytic	1593:1601	arg1	activity					1603:1610	its catalytic activity	1589:1610	its catalytic activity	1589:1610	To explore the source of galactan length variation, a C. diphtheriae ortholog of the M. tuberculosis carbohydrate polymerase responsible for the bulk of galactan polymerization, GlfT2, was produced, and its catalytic activity was evaluated.
28039359	9	10	dep	M.	1471:1472	arg1	tuberculosis					1474:1485	tuberculosis	1474:1485	tuberculosis	1474:1485	To explore the source of galactan length variation, a C. diphtheriae ortholog of the M. tuberculosis carbohydrate polymerase responsible for the bulk of galactan polymerization, GlfT2, was produced, and its catalytic activity was evaluated.
28039359	4	11	theme	biotechnological	661:676	arg1	applications					678:689	biotechnological applications	661:689	biotechnological applications	661:689	Elucidating microbe-specific differences in mAG composition could advance biotechnological applications and lead to new antimicrobial targets.
28039359	5	12	dep	contrast	758:765	arg1	biosynthesis					776:787	galactan biosynthesis	767:787	galactan biosynthesis	767:787	To this end, we compare and contrast galactan biosynthesis in C. diphtheriae and M. tuberculosis In each species, the galactan is constructed from uridine 5'-diphosphate-α-d-galactofuranose (UDP-Galf), which is generated by the enzyme UDP-galactopyranose mutase (UGM or Glf).
28039359	6	13	from	importance	1071:1080	arg1	species					1101:1107	Corynebacterium species	1085:1107	Corynebacterium species	1085:1107	UGM and the galactan are essential in M. tuberculosis, but their importance in Corynebacterium species was not known.
28039359	12	14	theme	cell	1933:1936	arg1	envelope					1938:1945	the Corynebacterineae cell envelope	1911:1945	the Corynebacterineae cell envelope	1911:1945	Our results provide tools, both small molecule and genetic, for probing and perturbing the assembly of the Corynebacterineae cell envelope.
28039359	9	15	theme	carbohydrate	1487:1498	arg1	polymerase					1500:1509	the M. tuberculosis carbohydrate polymerase	1467:1509	the M. tuberculosis carbohydrate polymerase responsible for the bulk of galactan polymerization, GlfT2,	1467:1569	To explore the source of galactan length variation, a C. diphtheriae ortholog of the M. tuberculosis carbohydrate polymerase responsible for the bulk of galactan polymerization, GlfT2, was produced, and its catalytic activity was evaluated.
28039359	2	16	theme	cell	406:409	arg1	envelope					411:418	the Corynebacterineae cell envelope	384:418	the Corynebacterineae cell envelope	384:418	A distinctive component of the Corynebacterineae cell envelope is the mycolyl-arabinogalactan (mAG) complex.
28039359	7	17	theme	molecule	1143:1150	arg1	inhibitors					1152:1161	small molecule inhibitors	1137:1161	small molecule inhibitors of UGM	1137:1168	We show that small molecule inhibitors of UGM impede C. glutamicum growth, suggesting that the galactan is critical in corynebacteria.
28039359	3	18	theme	carbohydrate	564:575	arg1	residues					577:584	carbohydrate residues	564:584	carbohydrate residues	564:584	The mAG is composed of lipid mycolic acids, and arabinofuranose (Araf) and galactofuranose (Galf) carbohydrate residues.
28039359	8	19	theme	galactan	1304:1311	arg1	polymer					1313:1319	the galactan polymer	1300:1319	the galactan polymer	1300:1319	Previous cell wall analysis data suggest the galactan polymer is longer in mycobacterial species than corynebacterial species.
28039359	1	20	theme	industrial	208:217	arg1	production					219:228	industrial production	208:228	industrial production of amino acids, as well as Corynebacterium diphtheriae and Mycobacterium tuberculosis, which cause devastating human diseases	208:354	The suborder Corynebacterineae encompasses species like Corynebacterium glutamicum, which has been harnessed for industrial production of amino acids, as well as Corynebacterium diphtheriae and Mycobacterium tuberculosis, which cause devastating human diseases.
28039359	5	21	dep	compare	746:752	arg1	constructed					860:870	constructed	860:870	is constructed from uridine 5'-diphosphate-α-d-galactofuranose (UDP-Galf), which is generated by the enzyme UDP-galactopyranose mutase (UGM or Glf)	857:1003	To this end, we compare and contrast galactan biosynthesis in C. diphtheriae and M. tuberculosis In each species, the galactan is constructed from uridine 5'-diphosphate-α-d-galactofuranose (UDP-Galf), which is generated by the enzyme UDP-galactopyranose mutase (UGM or Glf).
28039359	12	22	theme	envelope	1938:1945	arg1	assembly					1899:1906	the assembly	1895:1906	the assembly of the Corynebacterineae cell envelope	1895:1945	Our results provide tools, both small molecule and genetic, for probing and perturbing the assembly of the Corynebacterineae cell envelope.
28039359	1	23	theme	suborder	99:106	arg1	Corynebacterineae					108:124	The suborder Corynebacterineae	95:124	The suborder Corynebacterineae	95:124	The suborder Corynebacterineae encompasses species like Corynebacterium glutamicum, which has been harnessed for industrial production of amino acids, as well as Corynebacterium diphtheriae and Mycobacterium tuberculosis, which cause devastating human diseases.
28039359	3	24	theme	mycolic	495:501	arg1	acids					503:507	lipid mycolic acids	489:507	lipid mycolic acids	489:507	The mAG is composed of lipid mycolic acids, and arabinofuranose (Araf) and galactofuranose (Galf) carbohydrate residues.
28039359	0	25	theme	Galactan	10:17	arg1	Biosynthesis					19:30	Galactan Biosynthesis	10:30	Galactan Biosynthesis	10:30	Comparing Galactan Biosynthesis in Mycobacterium tuberculosis and Corynebacterium diphtheriae.
28039359	9	26	theme	galactan	1411:1418	arg1	variation					1427:1435	galactan length variation	1411:1435	galactan length variation	1411:1435	To explore the source of galactan length variation, a C. diphtheriae ortholog of the M. tuberculosis carbohydrate polymerase responsible for the bulk of galactan polymerization, GlfT2, was produced, and its catalytic activity was evaluated.
28039359	10	27	dep	C.	1631:1632	arg1	diphtheriae					1634:1644	diphtheriae	1634:1644	diphtheriae	1634:1644	The C. diphtheriae GlfT2 gave rise to shorter polysaccharides than those obtained with the M. tuberculosis GlfT2.
28039359	2	28	theme	mAG	452:454	arg1	complex					457:463	the mycolyl-arabinogalactan (mAG) complex	423:463	the mycolyl-arabinogalactan (mAG) complex	423:463	A distinctive component of the Corynebacterineae cell envelope is the mycolyl-arabinogalactan (mAG) complex.
28039359	2	28	theme	mAG	452:454	arg1	component					371:379	A distinctive component	357:379	A distinctive component of the Corynebacterineae cell envelope	357:418	A distinctive component of the Corynebacterineae cell envelope is the mycolyl-arabinogalactan (mAG) complex.
28039359	5	29	theme	uridine	877:883	arg1	UDP-Galf					921:928	UDP-Galf	921:928	UDP-Galf	921:928	To this end, we compare and contrast galactan biosynthesis in C. diphtheriae and M. tuberculosis In each species, the galactan is constructed from uridine 5'-diphosphate-α-d-galactofuranose (UDP-Galf), which is generated by the enzyme UDP-galactopyranose mutase (UGM or Glf).
28039359	5	29	theme	uridine	877:883	arg1	α-d-galactofuranose					900:918	uridine 5'-diphosphate-α-d-galactofuranose	877:918	uridine 5'-diphosphate-α-d-galactofuranose (UDP-Galf)	877:929	To this end, we compare and contrast galactan biosynthesis in C. diphtheriae and M. tuberculosis In each species, the galactan is constructed from uridine 5'-diphosphate-α-d-galactofuranose (UDP-Galf), which is generated by the enzyme UDP-galactopyranose mutase (UGM or Glf).
28039359	10	30	theme	shorter	1665:1671	arg1	polysaccharides					1673:1687	shorter polysaccharides	1665:1687	shorter polysaccharides	1665:1687	The C. diphtheriae GlfT2 gave rise to shorter polysaccharides than those obtained with the M. tuberculosis GlfT2.
28039359	6	31	from	tuberculosis	1047:1058	arg1	galactan					1018:1025	the galactan	1014:1025	the galactan	1014:1025	UGM and the galactan are essential in M. tuberculosis, but their importance in Corynebacterium species was not known.
28039359	6	31	from	tuberculosis	1047:1058	arg1	essential					1031:1039	essential	1031:1039	essential	1031:1039	UGM and the galactan are essential in M. tuberculosis, but their importance in Corynebacterium species was not known.
28039359	2	32	theme	distinctive	359:369	arg1	complex					457:463	the mycolyl-arabinogalactan (mAG) complex	423:463	the mycolyl-arabinogalactan (mAG) complex	423:463	A distinctive component of the Corynebacterineae cell envelope is the mycolyl-arabinogalactan (mAG) complex.
28039359	2	32	theme	distinctive	359:369	arg1	component					371:379	A distinctive component	357:379	A distinctive component of the Corynebacterineae cell envelope	357:418	A distinctive component of the Corynebacterineae cell envelope is the mycolyl-arabinogalactan (mAG) complex.
28039359	1	33	theme	amino	233:237	arg1	diphtheriae					273:283	Corynebacterium diphtheriae	257:283	Corynebacterium diphtheriae	257:283	The suborder Corynebacterineae encompasses species like Corynebacterium glutamicum, which has been harnessed for industrial production of amino acids, as well as Corynebacterium diphtheriae and Mycobacterium tuberculosis, which cause devastating human diseases.
28039359	1	33	theme	amino	233:237	arg1	tuberculosis					303:314	Mycobacterium tuberculosis	289:314	Mycobacterium tuberculosis	289:314	The suborder Corynebacterineae encompasses species like Corynebacterium glutamicum, which has been harnessed for industrial production of amino acids, as well as Corynebacterium diphtheriae and Mycobacterium tuberculosis, which cause devastating human diseases.
28039359	1	33	theme	amino	233:237	arg1	acids					239:243	amino acids	233:243	amino acids	233:243	The suborder Corynebacterineae encompasses species like Corynebacterium glutamicum, which has been harnessed for industrial production of amino acids, as well as Corynebacterium diphtheriae and Mycobacterium tuberculosis, which cause devastating human diseases.
28039359	4	34	from	differences	616:626	arg1	composition					635:645	mAG composition	631:645	mAG composition	631:645	Elucidating microbe-specific differences in mAG composition could advance biotechnological applications and lead to new antimicrobial targets.
28039359	1	35	theme	devastating	329:339	arg1	diseases					347:354	devastating human diseases	329:354	devastating human diseases	329:354	The suborder Corynebacterineae encompasses species like Corynebacterium glutamicum, which has been harnessed for industrial production of amino acids, as well as Corynebacterium diphtheriae and Mycobacterium tuberculosis, which cause devastating human diseases.
28039359	5	36	theme	galactan	767:774	arg1	biosynthesis					776:787	galactan biosynthesis	767:787	galactan biosynthesis	767:787	To this end, we compare and contrast galactan biosynthesis in C. diphtheriae and M. tuberculosis In each species, the galactan is constructed from uridine 5'-diphosphate-α-d-galactofuranose (UDP-Galf), which is generated by the enzyme UDP-galactopyranose mutase (UGM or Glf).
28039359	9	37	theme	polymerase	1500:1509	arg1	ortholog					1455:1462	a C. diphtheriae ortholog	1438:1462	a C. diphtheriae ortholog of the M. tuberculosis carbohydrate polymerase responsible for the bulk of galactan polymerization, GlfT2,	1438:1569	To explore the source of galactan length variation, a C. diphtheriae ortholog of the M. tuberculosis carbohydrate polymerase responsible for the bulk of galactan polymerization, GlfT2, was produced, and its catalytic activity was evaluated.
28039359	8	38	dep	suggest	1292:1298	arg1	longer					1324:1329	longer	1324:1329	longer	1324:1329	Previous cell wall analysis data suggest the galactan polymer is longer in mycobacterial species than corynebacterial species.
28039359	4	39	theme	antimicrobial	707:719	arg1	targets					721:727	new antimicrobial targets	703:727	new antimicrobial targets	703:727	Elucidating microbe-specific differences in mAG composition could advance biotechnological applications and lead to new antimicrobial targets.
28039359	5	40	dep	mutase	985:990	arg1	UGM					993:995	UGM	993:995	UGM	993:995	To this end, we compare and contrast galactan biosynthesis in C. diphtheriae and M. tuberculosis In each species, the galactan is constructed from uridine 5'-diphosphate-α-d-galactofuranose (UDP-Galf), which is generated by the enzyme UDP-galactopyranose mutase (UGM or Glf).
28039359	5	40	dep	mutase	985:990	arg1	Glf					1000:1002	Glf	1000:1002	Glf	1000:1002	To this end, we compare and contrast galactan biosynthesis in C. diphtheriae and M. tuberculosis In each species, the galactan is constructed from uridine 5'-diphosphate-α-d-galactofuranose (UDP-Galf), which is generated by the enzyme UDP-galactopyranose mutase (UGM or Glf).
28039359	1	41	theme	human	341:345	arg1	diseases					347:354	devastating human diseases	329:354	devastating human diseases	329:354	The suborder Corynebacterineae encompasses species like Corynebacterium glutamicum, which has been harnessed for industrial production of amino acids, as well as Corynebacterium diphtheriae and Mycobacterium tuberculosis, which cause devastating human diseases.
28039359	7	42	from	critical	1231:1238	arg1	corynebacteria					1243:1256	corynebacteria	1243:1256	corynebacteria	1243:1256	We show that small molecule inhibitors of UGM impede C. glutamicum growth, suggesting that the galactan is critical in corynebacteria.
28039359	4	43	theme	new	703:705	arg1	targets					721:727	new antimicrobial targets	703:727	new antimicrobial targets	703:727	Elucidating microbe-specific differences in mAG composition could advance biotechnological applications and lead to new antimicrobial targets.
28039359	8	44	theme	cell	1268:1271	arg1	data					1287:1290	Previous cell wall analysis data	1259:1290	Previous cell wall analysis data	1259:1290	Previous cell wall analysis data suggest the galactan polymer is longer in mycobacterial species than corynebacterial species.
28039359	8	45	theme	mycobacterial	1334:1346	arg1	species					1348:1354	mycobacterial species	1334:1354	mycobacterial species	1334:1354	Previous cell wall analysis data suggest the galactan polymer is longer in mycobacterial species than corynebacterial species.
28039359	6	46	from	essential	1031:1039	arg1	tuberculosis					1047:1058	M. tuberculosis	1044:1058	M. tuberculosis	1044:1058	UGM and the galactan are essential in M. tuberculosis, but their importance in Corynebacterium species was not known.
28039359	9	47	theme	galactan	1539:1546	arg1	GlfT2					1564:1568	GlfT2	1564:1568	GlfT2	1564:1568	To explore the source of galactan length variation, a C. diphtheriae ortholog of the M. tuberculosis carbohydrate polymerase responsible for the bulk of galactan polymerization, GlfT2, was produced, and its catalytic activity was evaluated.
28039359	9	47	theme	galactan	1539:1546	arg1	polymerization					1548:1561	galactan polymerization	1539:1561	galactan polymerization	1539:1561	To explore the source of galactan length variation, a C. diphtheriae ortholog of the M. tuberculosis carbohydrate polymerase responsible for the bulk of galactan polymerization, GlfT2, was produced, and its catalytic activity was evaluated.
28039359	3	48	theme	lipid	489:493	arg1	acids					503:507	lipid mycolic acids	489:507	lipid mycolic acids	489:507	The mAG is composed of lipid mycolic acids, and arabinofuranose (Araf) and galactofuranose (Galf) carbohydrate residues.
28039359	9	49	theme	length	1420:1425	arg1	variation					1427:1435	galactan length variation	1411:1435	galactan length variation	1411:1435	To explore the source of galactan length variation, a C. diphtheriae ortholog of the M. tuberculosis carbohydrate polymerase responsible for the bulk of galactan polymerization, GlfT2, was produced, and its catalytic activity was evaluated.
28039359	8	50	theme	Previous	1259:1266	arg1	data					1287:1290	Previous cell wall analysis data	1259:1290	Previous cell wall analysis data	1259:1290	Previous cell wall analysis data suggest the galactan polymer is longer in mycobacterial species than corynebacterial species.
28039359	7	51	from	corynebacteria	1243:1256	arg1	critical					1231:1238	critical	1231:1238	critical	1231:1238	We show that small molecule inhibitors of UGM impede C. glutamicum growth, suggesting that the galactan is critical in corynebacteria.
28039359	7	51	from	corynebacteria	1243:1256	arg1	galactan					1219:1226	the galactan	1215:1226	the galactan	1215:1226	We show that small molecule inhibitors of UGM impede C. glutamicum growth, suggesting that the galactan is critical in corynebacteria.
28039359	5	52	theme	5'-diphosphate-	885:899	arg1	UDP-Galf					921:928	UDP-Galf	921:928	UDP-Galf	921:928	To this end, we compare and contrast galactan biosynthesis in C. diphtheriae and M. tuberculosis In each species, the galactan is constructed from uridine 5'-diphosphate-α-d-galactofuranose (UDP-Galf), which is generated by the enzyme UDP-galactopyranose mutase (UGM or Glf).
28039359	5	52	theme	5'-diphosphate-	885:899	arg1	α-d-galactofuranose					900:918	uridine 5'-diphosphate-α-d-galactofuranose	877:918	uridine 5'-diphosphate-α-d-galactofuranose (UDP-Galf)	877:929	To this end, we compare and contrast galactan biosynthesis in C. diphtheriae and M. tuberculosis In each species, the galactan is constructed from uridine 5'-diphosphate-α-d-galactofuranose (UDP-Galf), which is generated by the enzyme UDP-galactopyranose mutase (UGM or Glf).
28039359	6	53	theme	Corynebacterium	1085:1099	arg1	species					1101:1107	Corynebacterium species	1085:1107	Corynebacterium species	1085:1107	UGM and the galactan are essential in M. tuberculosis, but their importance in Corynebacterium species was not known.
28039359	8	54	theme	analysis	1278:1285	arg1	data					1287:1290	Previous cell wall analysis data	1259:1290	Previous cell wall analysis data	1259:1290	Previous cell wall analysis data suggest the galactan polymer is longer in mycobacterial species than corynebacterial species.
28039359	9	55	theme	variation	1427:1435	arg1	source					1401:1406	the source	1397:1406	the source of galactan length variation	1397:1435	To explore the source of galactan length variation, a C. diphtheriae ortholog of the M. tuberculosis carbohydrate polymerase responsible for the bulk of galactan polymerization, GlfT2, was produced, and its catalytic activity was evaluated.
28039359	4	56	theme	microbe-specific	599:614	arg1	differences					616:626	microbe-specific differences	599:626	microbe-specific differences in mAG composition	599:645	Elucidating microbe-specific differences in mAG composition could advance biotechnological applications and lead to new antimicrobial targets.
28039359	7	57	dep	C.	1177:1178	arg1	glutamicum					1180:1189	glutamicum	1180:1189	glutamicum	1180:1189	We show that small molecule inhibitors of UGM impede C. glutamicum growth, suggesting that the galactan is critical in corynebacteria.
28039359	1	58	theme	Corynebacterium	151:165	arg1	glutamicum					167:176	Corynebacterium glutamicum	151:176	Corynebacterium glutamicum	151:176	The suborder Corynebacterineae encompasses species like Corynebacterium glutamicum, which has been harnessed for industrial production of amino acids, as well as Corynebacterium diphtheriae and Mycobacterium tuberculosis, which cause devastating human diseases.
28039359	8	59	theme	wall	1273:1276	arg1	data					1287:1290	Previous cell wall analysis data	1259:1290	Previous cell wall analysis data	1259:1290	Previous cell wall analysis data suggest the galactan polymer is longer in mycobacterial species than corynebacterial species.
28039359	12	60	theme	Corynebacterineae	1915:1931	arg1	envelope					1938:1945	the Corynebacterineae cell envelope	1911:1945	the Corynebacterineae cell envelope	1911:1945	Our results provide tools, both small molecule and genetic, for probing and perturbing the assembly of the Corynebacterineae cell envelope.
28039359	2	61	theme	Corynebacterineae	388:404	arg1	envelope					411:418	the Corynebacterineae cell envelope	384:418	the Corynebacterineae cell envelope	384:418	A distinctive component of the Corynebacterineae cell envelope is the mycolyl-arabinogalactan (mAG) complex.
28039359	11	62	theme	galactan	1791:1798	arg1	length					1800:1805	galactan length	1791:1805	galactan length	1791:1805	These data suggest that GlfT2 alone can influence galactan length.
28039359	8	63	theme	corynebacterial	1361:1375	arg1	species					1377:1383	corynebacterial species	1361:1383	corynebacterial species	1361:1383	Previous cell wall analysis data suggest the galactan polymer is longer in mycobacterial species than corynebacterial species.
28039359	9	64	theme	C.	1440:1441	arg1	ortholog					1455:1462	a C. diphtheriae ortholog	1438:1462	a C. diphtheriae ortholog of the M. tuberculosis carbohydrate polymerase responsible for the bulk of galactan polymerization, GlfT2,	1438:1569	To explore the source of galactan length variation, a C. diphtheriae ortholog of the M. tuberculosis carbohydrate polymerase responsible for the bulk of galactan polymerization, GlfT2, was produced, and its catalytic activity was evaluated.
28039359	10	65	theme	tuberculosis	1721:1732	arg1	GlfT2					1734:1738	the M. tuberculosis GlfT2	1714:1738	the M. tuberculosis GlfT2	1714:1738	The C. diphtheriae GlfT2 gave rise to shorter polysaccharides than those obtained with the M. tuberculosis GlfT2.
28039359	7	66	theme	small	1137:1141	arg1	inhibitors					1152:1161	small molecule inhibitors	1137:1161	small molecule inhibitors of UGM	1137:1168	We show that small molecule inhibitors of UGM impede C. glutamicum growth, suggesting that the galactan is critical in corynebacteria.
28039359	12	67	theme	small	1840:1844	arg1	molecule					1846:1853	small molecule	1840:1853	small molecule	1840:1853	Our results provide tools, both small molecule and genetic, for probing and perturbing the assembly of the Corynebacterineae cell envelope.
28039359	9	68	theme	polymerization	1548:1561	arg1	bulk					1531:1534	the bulk	1527:1534	the bulk of galactan polymerization, GlfT2,	1527:1569	To explore the source of galactan length variation, a C. diphtheriae ortholog of the M. tuberculosis carbohydrate polymerase responsible for the bulk of galactan polymerization, GlfT2, was produced, and its catalytic activity was evaluated.
28039359	1	69	theme	acids	239:243	arg1	production					219:228	industrial production	208:228	industrial production of amino acids, as well as Corynebacterium diphtheriae and Mycobacterium tuberculosis, which cause devastating human diseases	208:354	The suborder Corynebacterineae encompasses species like Corynebacterium glutamicum, which has been harnessed for industrial production of amino acids, as well as Corynebacterium diphtheriae and Mycobacterium tuberculosis, which cause devastating human diseases.
28039359	2	70	theme	mycolyl-arabinogalactan	427:449	arg1	complex					457:463	the mycolyl-arabinogalactan (mAG) complex	423:463	the mycolyl-arabinogalactan (mAG) complex	423:463	A distinctive component of the Corynebacterineae cell envelope is the mycolyl-arabinogalactan (mAG) complex.
28039359	2	70	theme	mycolyl-arabinogalactan	427:449	arg1	component					371:379	A distinctive component	357:379	A distinctive component of the Corynebacterineae cell envelope	357:418	A distinctive component of the Corynebacterineae cell envelope is the mycolyl-arabinogalactan (mAG) complex.
28039359	9	71	theme	responsible	1511:1521	arg1	polymerase					1500:1509	the M. tuberculosis carbohydrate polymerase	1467:1509	the M. tuberculosis carbohydrate polymerase responsible for the bulk of galactan polymerization, GlfT2,	1467:1569	To explore the source of galactan length variation, a C. diphtheriae ortholog of the M. tuberculosis carbohydrate polymerase responsible for the bulk of galactan polymerization, GlfT2, was produced, and its catalytic activity was evaluated.
28039359	6	72	theme	M.	1044:1045	arg1	tuberculosis					1047:1058	M. tuberculosis	1044:1058	M. tuberculosis	1044:1058	UGM and the galactan are essential in M. tuberculosis, but their importance in Corynebacterium species was not known.
28039359	3	73	dep	arabinofuranose	514:528	arg1	residues					577:584	carbohydrate residues	564:584	carbohydrate residues	564:584	The mAG is composed of lipid mycolic acids, and arabinofuranose (Araf) and galactofuranose (Galf) carbohydrate residues.
28039359	5	74	theme	enzyme	958:963	arg1	mutase					985:990	the enzyme UDP-galactopyranose mutase	954:990	the enzyme UDP-galactopyranose mutase (UGM or Glf)	954:1003	To this end, we compare and contrast galactan biosynthesis in C. diphtheriae and M. tuberculosis In each species, the galactan is constructed from uridine 5'-diphosphate-α-d-galactofuranose (UDP-Galf), which is generated by the enzyme UDP-galactopyranose mutase (UGM or Glf).
28039359	12	75	dep	both	1835:1838	arg1	molecule					1846:1853	small molecule	1840:1853	small molecule	1840:1853	Our results provide tools, both small molecule and genetic, for probing and perturbing the assembly of the Corynebacterineae cell envelope.
28039359	10	76	theme	C.	1631:1632	arg1	GlfT2					1646:1650	The C. diphtheriae GlfT2	1627:1650	The C. diphtheriae GlfT2	1627:1650	The C. diphtheriae GlfT2 gave rise to shorter polysaccharides than those obtained with the M. tuberculosis GlfT2.
28183605	0	0	theme	acid	87:90	arg1	release					59:65	modified release	50:65	modified release of 5-aminosalicylic acid	50:90	Obtaining the palygorskite:chitosan composite for modified release of 5-aminosalicylic acid.
28183605	7	1	theme	modified	1225:1232	arg1	release					1234:1240	effective modified release	1215:1240	5-ASA effective modified release	1209:1240	Thus, the PLG:CS composite with proven synergy of the PLG and CS inherent properties showing 5-ASA effective modified release.
28183605	6	2	theme	analyzed	1043:1050	arg1	fluids					1062:1067	analyzed simulated fluids	1043:1067	analyzed simulated fluids (intestinal and stomach)	1043:1092	The release profiles show that the PLG:CS composite changed the 5-ASA release speed in analyzed simulated fluids (intestinal and stomach) unlike other systems.
28183605	6	3	theme	simulated	1052:1060	arg1	fluids					1062:1067	analyzed simulated fluids	1043:1067	analyzed simulated fluids (intestinal and stomach)	1043:1092	The release profiles show that the PLG:CS composite changed the 5-ASA release speed in analyzed simulated fluids (intestinal and stomach) unlike other systems.
28183605	7	4	theme	PLG	1126:1128	arg1	CS					1130:1131	the PLG:CS	1122:1131	the PLG:CS	1122:1131	Thus, the PLG:CS composite with proven synergy of the PLG and CS inherent properties showing 5-ASA effective modified release.
28183605	0	5	theme	5-aminosalicylic	70:85	arg1	acid					87:90	5-aminosalicylic acid	70:90	5-aminosalicylic acid	70:90	Obtaining the palygorskite:chitosan composite for modified release of 5-aminosalicylic acid.
28183605	4	6	theme	morphological	666:678	arg1	area					660:663	specific surface area	643:663	specific surface area	643:663	Based on analytical data, the formation of the PLG:CS composite which showed the presence about 19% of CS, decrease in specific surface area, morphological analysis modified, visible change of crystallinity, of FTIR and thermal analysis.
28183605	4	6	theme	morphological	666:678	arg1	analysis					680:687	morphological analysis	666:687	morphological analysis modified	666:696	Based on analytical data, the formation of the PLG:CS composite which showed the presence about 19% of CS, decrease in specific surface area, morphological analysis modified, visible change of crystallinity, of FTIR and thermal analysis.
28183605	4	7	theme	visible	699:705	arg1	change					707:712	visible change	699:712	visible change	699:712	Based on analytical data, the formation of the PLG:CS composite which showed the presence about 19% of CS, decrease in specific surface area, morphological analysis modified, visible change of crystallinity, of FTIR and thermal analysis.
28183605	4	7	theme	visible	699:705	arg1	area					660:663	specific surface area	643:663	specific surface area	643:663	Based on analytical data, the formation of the PLG:CS composite which showed the presence about 19% of CS, decrease in specific surface area, morphological analysis modified, visible change of crystallinity, of FTIR and thermal analysis.
28183605	5	8	theme	significant	828:838	arg1	increase					840:847	a significant increase	826:847	a significant increase in adsorption with 5-ASA	826:872	In relation to the drug-composite interaction, PLG:CS exhibited a significant increase in adsorption with 5-ASA at 58.24% in relation to PLG and CS which were at 16.29% and 23.96% respectively.
28183605	3	9	theme	surface	434:440	arg1	analysis					447:454	surface area analysis	434:454	surface area analysis	434:454	Then, PLG, CS and PLG:CS were characterized by means of analytical techniques such as CHN elemental analysis, surface area analysis, XRD, FTIR, DSC and TG, SEM, adsorption tests and release profiles.
28183605	4	10	theme	composite	578:586	arg1	formation					554:562	the formation	550:562	the formation	550:562	Based on analytical data, the formation of the PLG:CS composite which showed the presence about 19% of CS, decrease in specific surface area, morphological analysis modified, visible change of crystallinity, of FTIR and thermal analysis.
28183605	4	11	theme	analysis	752:759	arg1	change					707:712	visible change	699:712	visible change	699:712	Based on analytical data, the formation of the PLG:CS composite which showed the presence about 19% of CS, decrease in specific surface area, morphological analysis modified, visible change of crystallinity, of FTIR and thermal analysis.
28183605	4	11	theme	analysis	752:759	arg1	area					660:663	specific surface area	643:663	specific surface area	643:663	Based on analytical data, the formation of the PLG:CS composite which showed the presence about 19% of CS, decrease in specific surface area, morphological analysis modified, visible change of crystallinity, of FTIR and thermal analysis.
28183605	7	12	theme	inherent	1181:1188	arg1	properties					1190:1199	inherent properties	1181:1199	inherent properties	1181:1199	Thus, the PLG:CS composite with proven synergy of the PLG and CS inherent properties showing 5-ASA effective modified release.
28183605	7	13	theme	PLG	1170:1172	arg1	synergy					1155:1161	proven synergy	1148:1161	proven synergy of the PLG and CS inherent properties showing 5-ASA effective modified release	1148:1240	Thus, the PLG:CS composite with proven synergy of the PLG and CS inherent properties showing 5-ASA effective modified release.
28183605	3	14	theme	area	442:445	arg1	analysis					447:454	surface area analysis	434:454	surface area analysis	434:454	Then, PLG, CS and PLG:CS were characterized by means of analytical techniques such as CHN elemental analysis, surface area analysis, XRD, FTIR, DSC and TG, SEM, adsorption tests and release profiles.
28183605	8	15	contain	has	1265:1267	arg1	composite					1255:1263	this composite	1250:1263	this composite	1250:1263	Hence, this composite has potential benefits for the vectorization of drugs.
28183605	8	15	contain	has	1265:1267	arg2	benefits					1279:1286	potential benefits	1269:1286	potential benefits for the vectorization of drugs	1269:1317	Hence, this composite has potential benefits for the vectorization of drugs.
28183605	7	16	dep	PLG	1170:1172	arg1	properties					1190:1199	inherent properties	1181:1199	inherent properties	1181:1199	Thus, the PLG:CS composite with proven synergy of the PLG and CS inherent properties showing 5-ASA effective modified release.
28183605	7	16	dep	PLG	1170:1172	arg1	the					1166:1168	the	1166:1168	the	1166:1168	Thus, the PLG:CS composite with proven synergy of the PLG and CS inherent properties showing 5-ASA effective modified release.
28183605	6	17	theme	5-ASA	1020:1024	arg1	release					1026:1032	the 5-ASA release	1016:1032	the 5-ASA release speed in analyzed simulated fluids (intestinal and stomach)	1016:1092	The release profiles show that the PLG:CS composite changed the 5-ASA release speed in analyzed simulated fluids (intestinal and stomach) unlike other systems.
28183605	5	18	theme	drug-composite	781:794	arg1	interaction					796:806	the drug-composite interaction	777:806	the drug-composite interaction	777:806	In relation to the drug-composite interaction, PLG:CS exhibited a significant increase in adsorption with 5-ASA at 58.24% in relation to PLG and CS which were at 16.29% and 23.96% respectively.
28183605	6	19	theme	other	1101:1105	arg1	systems					1107:1113	other systems	1101:1113	other systems	1101:1113	The release profiles show that the PLG:CS composite changed the 5-ASA release speed in analyzed simulated fluids (intestinal and stomach) unlike other systems.
28183605	8	20	theme	potential	1269:1277	arg1	benefits					1279:1286	potential benefits	1269:1286	potential benefits for the vectorization of drugs	1269:1317	Hence, this composite has potential benefits for the vectorization of drugs.
28183605	4	21	theme	surface	652:658	arg1	change					707:712	visible change	699:712	visible change	699:712	Based on analytical data, the formation of the PLG:CS composite which showed the presence about 19% of CS, decrease in specific surface area, morphological analysis modified, visible change of crystallinity, of FTIR and thermal analysis.
28183605	4	21	theme	surface	652:658	arg1	area					660:663	specific surface area	643:663	specific surface area	643:663	Based on analytical data, the formation of the PLG:CS composite which showed the presence about 19% of CS, decrease in specific surface area, morphological analysis modified, visible change of crystallinity, of FTIR and thermal analysis.
28183605	4	21	theme	surface	652:658	arg1	analysis					680:687	morphological analysis	666:687	morphological analysis modified	666:696	Based on analytical data, the formation of the PLG:CS composite which showed the presence about 19% of CS, decrease in specific surface area, morphological analysis modified, visible change of crystallinity, of FTIR and thermal analysis.
28183605	6	22	dep	fluids	1062:1067	arg1	stomach					1085:1091	stomach	1085:1091	stomach	1085:1091	The release profiles show that the PLG:CS composite changed the 5-ASA release speed in analyzed simulated fluids (intestinal and stomach) unlike other systems.
28183605	6	22	dep	fluids	1062:1067	arg1	intestinal					1070:1079	intestinal	1070:1079	intestinal	1070:1079	The release profiles show that the PLG:CS composite changed the 5-ASA release speed in analyzed simulated fluids (intestinal and stomach) unlike other systems.
28183605	7	23	theme	CS	1178:1179	arg1	synergy					1155:1161	proven synergy	1148:1161	proven synergy of the PLG and CS inherent properties showing 5-ASA effective modified release	1148:1240	Thus, the PLG:CS composite with proven synergy of the PLG and CS inherent properties showing 5-ASA effective modified release.
28183605	3	24	dep	PLG	330:332	arg1	CS					346:347	CS	346:347	PLG, CS and PLG:CS	330:347	Then, PLG, CS and PLG:CS were characterized by means of analytical techniques such as CHN elemental analysis, surface area analysis, XRD, FTIR, DSC and TG, SEM, adsorption tests and release profiles.
28183605	4	25	theme	CS	575:576	arg1	composite					578:586	the PLG:CS composite	567:586	the PLG:CS composite	567:586	Based on analytical data, the formation of the PLG:CS composite which showed the presence about 19% of CS, decrease in specific surface area, morphological analysis modified, visible change of crystallinity, of FTIR and thermal analysis.
28183605	5	26	with	adsorption	852:861	arg1	5-ASA					868:872	5-ASA	868:872	5-ASA	868:872	In relation to the drug-composite interaction, PLG:CS exhibited a significant increase in adsorption with 5-ASA at 58.24% in relation to PLG and CS which were at 16.29% and 23.96% respectively.
28183605	2	27	theme	reticular	307:315	arg1	agent					317:321	a reticular agent	305:321	a reticular agent	305:321	Initially, the PLG:CS composite was obtained using glutaraldehyde (GLA) as a reticular agent.
28183605	2	27	theme	reticular	307:315	arg1	glutaraldehyde					281:294	glutaraldehyde	281:294	glutaraldehyde (GLA)	281:300	Initially, the PLG:CS composite was obtained using glutaraldehyde (GLA) as a reticular agent.
28183605	7	28	with	composite	1133:1141	arg1	synergy					1155:1161	proven synergy	1148:1161	proven synergy of the PLG and CS inherent properties showing 5-ASA effective modified release	1148:1240	Thus, the PLG:CS composite with proven synergy of the PLG and CS inherent properties showing 5-ASA effective modified release.
28183605	2	29	theme	CS	249:250	arg1	composite					252:260	the PLG:CS composite	241:260	the PLG:CS composite	241:260	Initially, the PLG:CS composite was obtained using glutaraldehyde (GLA) as a reticular agent.
28183605	3	30	theme	release	506:512	arg1	profiles					514:521	release profiles	506:521	release profiles	506:521	Then, PLG, CS and PLG:CS were characterized by means of analytical techniques such as CHN elemental analysis, surface area analysis, XRD, FTIR, DSC and TG, SEM, adsorption tests and release profiles.
28183605	7	31	theme	proven	1148:1153	arg1	synergy					1155:1161	proven synergy	1148:1161	proven synergy of the PLG and CS inherent properties showing 5-ASA effective modified release	1148:1240	Thus, the PLG:CS composite with proven synergy of the PLG and CS inherent properties showing 5-ASA effective modified release.
28183605	4	32	theme	FTIR	735:738	arg1	analysis					752:759	FTIR and thermal analysis	735:759	FTIR and thermal analysis	735:759	Based on analytical data, the formation of the PLG:CS composite which showed the presence about 19% of CS, decrease in specific surface area, morphological analysis modified, visible change of crystallinity, of FTIR and thermal analysis.
28183605	5	33	from	increase	840:847	arg1	adsorption					852:861	adsorption	852:861	adsorption with 5-ASA	852:872	In relation to the drug-composite interaction, PLG:CS exhibited a significant increase in adsorption with 5-ASA at 58.24% in relation to PLG and CS which were at 16.29% and 23.96% respectively.
28183605	0	34	theme	palygorskite	14:25	arg1	composite					36:44	the palygorskite:chitosan composite	10:44	the palygorskite:chitosan composite	10:44	Obtaining the palygorskite:chitosan composite for modified release of 5-aminosalicylic acid.
28183605	2	35	theme	PLG	245:247	arg1	composite					252:260	the PLG:CS composite	241:260	the PLG:CS composite	241:260	Initially, the PLG:CS composite was obtained using glutaraldehyde (GLA) as a reticular agent.
28183605	4	36	theme	thermal	744:750	arg1	analysis					752:759	FTIR and thermal analysis	735:759	FTIR and thermal analysis	735:759	Based on analytical data, the formation of the PLG:CS composite which showed the presence about 19% of CS, decrease in specific surface area, morphological analysis modified, visible change of crystallinity, of FTIR and thermal analysis.
28183605	1	37	dep	5-aminosalicylic	196:211	arg1	5-ASA					214:218	5-ASA	214:218	5-ASA	214:218	This study's aim was to obtain composites from palygorskite (PLG) and chitosan (CS) in order to modify 5-aminosalicylic (5-ASA) release.
28183605	6	38	theme	CS	995:996	arg1	composite					998:1006	the PLG:CS composite	987:1006	the PLG:CS composite	987:1006	The release profiles show that the PLG:CS composite changed the 5-ASA release speed in analyzed simulated fluids (intestinal and stomach) unlike other systems.
28183605	0	39	theme	chitosan	27:34	arg1	composite					36:44	the palygorskite:chitosan composite	10:44	the palygorskite:chitosan composite	10:44	Obtaining the palygorskite:chitosan composite for modified release of 5-aminosalicylic acid.
28183605	8	40	theme	drugs	1313:1317	arg1	vectorization					1296:1308	the vectorization	1292:1308	the vectorization of drugs	1292:1317	Hence, this composite has potential benefits for the vectorization of drugs.
28183605	3	41	theme	adsorption	485:494	arg1	tests					496:500	adsorption tests	485:500	adsorption tests	485:500	Then, PLG, CS and PLG:CS were characterized by means of analytical techniques such as CHN elemental analysis, surface area analysis, XRD, FTIR, DSC and TG, SEM, adsorption tests and release profiles.
28183605	5	42	dep	PLG	809:811	arg1	CS					813:814	CS	813:814	PLG:CS	809:814	In relation to the drug-composite interaction, PLG:CS exhibited a significant increase in adsorption with 5-ASA at 58.24% in relation to PLG and CS which were at 16.29% and 23.96% respectively.
28183605	0	43	theme	modified	50:57	arg1	release					59:65	modified release	50:65	modified release of 5-aminosalicylic acid	50:90	Obtaining the palygorskite:chitosan composite for modified release of 5-aminosalicylic acid.
28183605	7	44	dep	5-ASA	1209:1213	arg1	release					1234:1240	effective modified release	1215:1240	5-ASA effective modified release	1209:1240	Thus, the PLG:CS composite with proven synergy of the PLG and CS inherent properties showing 5-ASA effective modified release.
28183605	4	45	theme	specific	643:650	arg1	change					707:712	visible change	699:712	visible change	699:712	Based on analytical data, the formation of the PLG:CS composite which showed the presence about 19% of CS, decrease in specific surface area, morphological analysis modified, visible change of crystallinity, of FTIR and thermal analysis.
28183605	4	45	theme	specific	643:650	arg1	area					660:663	specific surface area	643:663	specific surface area	643:663	Based on analytical data, the formation of the PLG:CS composite which showed the presence about 19% of CS, decrease in specific surface area, morphological analysis modified, visible change of crystallinity, of FTIR and thermal analysis.
28183605	4	45	theme	specific	643:650	arg1	analysis					680:687	morphological analysis	666:687	morphological analysis modified	666:696	Based on analytical data, the formation of the PLG:CS composite which showed the presence about 19% of CS, decrease in specific surface area, morphological analysis modified, visible change of crystallinity, of FTIR and thermal analysis.
28183605	3	46	theme	analytical	380:389	arg1	DSC					468:470	DSC	468:470	DSC	468:470	Then, PLG, CS and PLG:CS were characterized by means of analytical techniques such as CHN elemental analysis, surface area analysis, XRD, FTIR, DSC and TG, SEM, adsorption tests and release profiles.
28183605	3	46	theme	analytical	380:389	arg1	tests					496:500	adsorption tests	485:500	adsorption tests	485:500	Then, PLG, CS and PLG:CS were characterized by means of analytical techniques such as CHN elemental analysis, surface area analysis, XRD, FTIR, DSC and TG, SEM, adsorption tests and release profiles.
28183605	3	46	theme	analytical	380:389	arg1	analysis					447:454	surface area analysis	434:454	surface area analysis	434:454	Then, PLG, CS and PLG:CS were characterized by means of analytical techniques such as CHN elemental analysis, surface area analysis, XRD, FTIR, DSC and TG, SEM, adsorption tests and release profiles.
28183605	3	46	theme	analytical	380:389	arg1	XRD					457:459	XRD	457:459	XRD	457:459	Then, PLG, CS and PLG:CS were characterized by means of analytical techniques such as CHN elemental analysis, surface area analysis, XRD, FTIR, DSC and TG, SEM, adsorption tests and release profiles.
28183605	3	46	theme	analytical	380:389	arg1	analysis					424:431	CHN elemental analysis	410:431	CHN elemental analysis	410:431	Then, PLG, CS and PLG:CS were characterized by means of analytical techniques such as CHN elemental analysis, surface area analysis, XRD, FTIR, DSC and TG, SEM, adsorption tests and release profiles.
28183605	3	46	theme	analytical	380:389	arg1	techniques					391:400	analytical techniques	380:400	analytical techniques such as CHN elemental analysis, surface area analysis, XRD, FTIR, DSC and TG, SEM, adsorption tests and release profiles	380:521	Then, PLG, CS and PLG:CS were characterized by means of analytical techniques such as CHN elemental analysis, surface area analysis, XRD, FTIR, DSC and TG, SEM, adsorption tests and release profiles.
28183605	3	46	theme	analytical	380:389	arg1	SEM					480:482	SEM	480:482	SEM	480:482	Then, PLG, CS and PLG:CS were characterized by means of analytical techniques such as CHN elemental analysis, surface area analysis, XRD, FTIR, DSC and TG, SEM, adsorption tests and release profiles.
28183605	3	46	theme	analytical	380:389	arg1	profiles					514:521	release profiles	506:521	release profiles	506:521	Then, PLG, CS and PLG:CS were characterized by means of analytical techniques such as CHN elemental analysis, surface area analysis, XRD, FTIR, DSC and TG, SEM, adsorption tests and release profiles.
28183605	3	46	theme	analytical	380:389	arg1	FTIR					462:465	FTIR	462:465	FTIR	462:465	Then, PLG, CS and PLG:CS were characterized by means of analytical techniques such as CHN elemental analysis, surface area analysis, XRD, FTIR, DSC and TG, SEM, adsorption tests and release profiles.
28183605	5	47	dep	interaction	796:806	arg1	relation					765:772	relation	765:772	relation	765:772	In relation to the drug-composite interaction, PLG:CS exhibited a significant increase in adsorption with 5-ASA at 58.24% in relation to PLG and CS which were at 16.29% and 23.96% respectively.
28183605	4	48	theme	PLG	571:573	arg1	composite					578:586	the PLG:CS composite	567:586	the PLG:CS composite	567:586	Based on analytical data, the formation of the PLG:CS composite which showed the presence about 19% of CS, decrease in specific surface area, morphological analysis modified, visible change of crystallinity, of FTIR and thermal analysis.
28183605	4	49	theme	crystallinity	717:729	arg1	change					707:712	visible change	699:712	visible change	699:712	Based on analytical data, the formation of the PLG:CS composite which showed the presence about 19% of CS, decrease in specific surface area, morphological analysis modified, visible change of crystallinity, of FTIR and thermal analysis.
28183605	4	49	theme	crystallinity	717:729	arg1	area					660:663	specific surface area	643:663	specific surface area	643:663	Based on analytical data, the formation of the PLG:CS composite which showed the presence about 19% of CS, decrease in specific surface area, morphological analysis modified, visible change of crystallinity, of FTIR and thermal analysis.
28183605	6	50	theme	PLG	991:993	arg1	composite					998:1006	the PLG:CS composite	987:1006	the PLG:CS composite	987:1006	The release profiles show that the PLG:CS composite changed the 5-ASA release speed in analyzed simulated fluids (intestinal and stomach) unlike other systems.
28183605	4	51	theme	analytical	533:542	arg1	data					544:547	analytical data	533:547	analytical data	533:547	Based on analytical data, the formation of the PLG:CS composite which showed the presence about 19% of CS, decrease in specific surface area, morphological analysis modified, visible change of crystallinity, of FTIR and thermal analysis.
28183605	4	52	theme	CS	627:628	arg1	CS					627:628	CS	627:628	CS	627:628	Based on analytical data, the formation of the PLG:CS composite which showed the presence about 19% of CS, decrease in specific surface area, morphological analysis modified, visible change of crystallinity, of FTIR and thermal analysis.
28183605	4	52	theme	CS	627:628	arg1	%					622:622	19%	620:622	19% of CS	620:628	Based on analytical data, the formation of the PLG:CS composite which showed the presence about 19% of CS, decrease in specific surface area, morphological analysis modified, visible change of crystallinity, of FTIR and thermal analysis.
28183605	3	53	theme	CHN	410:412	arg1	analysis					424:431	CHN elemental analysis	410:431	CHN elemental analysis	410:431	Then, PLG, CS and PLG:CS were characterized by means of analytical techniques such as CHN elemental analysis, surface area analysis, XRD, FTIR, DSC and TG, SEM, adsorption tests and release profiles.
28183605	7	54	theme	effective	1215:1223	arg1	release					1234:1240	effective modified release	1215:1240	5-ASA effective modified release	1209:1240	Thus, the PLG:CS composite with proven synergy of the PLG and CS inherent properties showing 5-ASA effective modified release.
28183605	6	55	theme	release	960:966	arg1	profiles					968:975	The release profiles	956:975	The release profiles	956:975	The release profiles show that the PLG:CS composite changed the 5-ASA release speed in analyzed simulated fluids (intestinal and stomach) unlike other systems.
28183605	3	56	theme	elemental	414:422	arg1	analysis					424:431	CHN elemental analysis	410:431	CHN elemental analysis	410:431	Then, PLG, CS and PLG:CS were characterized by means of analytical techniques such as CHN elemental analysis, surface area analysis, XRD, FTIR, DSC and TG, SEM, adsorption tests and release profiles.
28183605	1	57	theme	5-aminosalicylic	196:211	arg1	release					221:227	5-aminosalicylic (5-ASA) release	196:227	5-aminosalicylic (5-ASA) release	196:227	This study's aim was to obtain composites from palygorskite (PLG) and chitosan (CS) in order to modify 5-aminosalicylic (5-ASA) release.
24364596	0	0	theme	chromatography-mass	83:101	arg1	spectrometry					103:114	liquid chromatography-mass spectrometry	76:114	liquid chromatography-mass spectrometry	76:114	Method to detect contaminants in heparin using radical depolymerization and liquid chromatography-mass spectrometry.
24364596	3	1	theme	chondroitin	738:748	arg1	sulfate					750:756	N-sulfo oversulfated chondroitin sulfate	717:756	N-sulfo oversulfated chondroitin sulfate	717:756	This study synthesizes this new potential contaminant and describes the use of radical depolymerization followed by liquid chromatography-mass spectrometry to detect N-sulfo oversulfated chondroitin sulfate and to confirm the structure of the 2008 contaminant as oversulfated chondroitin sulfate and not oversulfated heparan sulfate.
24364596	1	2	theme	severe	262:267	arg1	some					288:291	some	288:291	some	288:291	Heparin is a critically important anticoagulant drug that was contaminated with a persulfonated polysaccharide in 2008, resulting in a number of severe adverse reactions, some leading to death.
24364596	1	2	theme	severe	262:267	arg1	reactions					277:285	severe adverse reactions	262:285	severe adverse reactions	262:285	Heparin is a critically important anticoagulant drug that was contaminated with a persulfonated polysaccharide in 2008, resulting in a number of severe adverse reactions, some leading to death.
24364596	0	3	theme	liquid	76:81	arg1	spectrometry					103:114	liquid chromatography-mass spectrometry	76:114	liquid chromatography-mass spectrometry	76:114	Method to detect contaminants in heparin using radical depolymerization and liquid chromatography-mass spectrometry.
24364596	1	4	theme	adverse	269:275	arg1	some					288:291	some	288:291	some	288:291	Heparin is a critically important anticoagulant drug that was contaminated with a persulfonated polysaccharide in 2008, resulting in a number of severe adverse reactions, some leading to death.
24364596	1	4	theme	adverse	269:275	arg1	reactions					277:285	severe adverse reactions	262:285	severe adverse reactions	262:285	Heparin is a critically important anticoagulant drug that was contaminated with a persulfonated polysaccharide in 2008, resulting in a number of severe adverse reactions, some leading to death.
24364596	3	5	theme	N-sulfo	717:723	arg1	sulfate					750:756	N-sulfo oversulfated chondroitin sulfate	717:756	N-sulfo oversulfated chondroitin sulfate	717:756	This study synthesizes this new potential contaminant and describes the use of radical depolymerization followed by liquid chromatography-mass spectrometry to detect N-sulfo oversulfated chondroitin sulfate and to confirm the structure of the 2008 contaminant as oversulfated chondroitin sulfate and not oversulfated heparan sulfate.
24364596	2	6	dep	new	470:472	arg1	contaminant					496:506	contaminant	496:506	contaminant	496:506	Controversy remains as to the precise composition of the 2008 contaminant, and new information suggests that heparin may now be subject to adulteration with a new, difficult to detect, contaminant, N-sulfo oversulfated chondroitin sulfate.
24364596	2	6	dep	new	470:472	arg1	oversulfated					517:528	oversulfated	517:528	oversulfated	517:528	Controversy remains as to the precise composition of the 2008 contaminant, and new information suggests that heparin may now be subject to adulteration with a new, difficult to detect, contaminant, N-sulfo oversulfated chondroitin sulfate.
24364596	2	6	dep	new	470:472	arg1	difficult					475:483	difficult	475:483	difficult	475:483	Controversy remains as to the precise composition of the 2008 contaminant, and new information suggests that heparin may now be subject to adulteration with a new, difficult to detect, contaminant, N-sulfo oversulfated chondroitin sulfate.
24364596	1	7	theme	reactions	277:285	arg1	number					252:257	a number	250:257	a number of severe adverse reactions, some leading to death	250:308	Heparin is a critically important anticoagulant drug that was contaminated with a persulfonated polysaccharide in 2008, resulting in a number of severe adverse reactions, some leading to death.
24364596	3	8	theme	heparan	868:874	arg1	sulfate					876:882	not oversulfated heparan sulfate	851:882	not oversulfated heparan sulfate	851:882	This study synthesizes this new potential contaminant and describes the use of radical depolymerization followed by liquid chromatography-mass spectrometry to detect N-sulfo oversulfated chondroitin sulfate and to confirm the structure of the 2008 contaminant as oversulfated chondroitin sulfate and not oversulfated heparan sulfate.
24364596	2	9	theme	new	390:392	arg1	information					394:404	new information	390:404	new information	390:404	Controversy remains as to the precise composition of the 2008 contaminant, and new information suggests that heparin may now be subject to adulteration with a new, difficult to detect, contaminant, N-sulfo oversulfated chondroitin sulfate.
24364596	1	10	theme	persulfonated	199:211	arg1	polysaccharide					213:226	a persulfonated polysaccharide	197:226	a persulfonated polysaccharide in 2008	197:234	Heparin is a critically important anticoagulant drug that was contaminated with a persulfonated polysaccharide in 2008, resulting in a number of severe adverse reactions, some leading to death.
24364596	2	11	with	adulteration	450:461	arg1	sulfate					542:548	a new, difficult to detect, contaminant, N-sulfo oversulfated chondroitin sulfate	468:548	a new, difficult to detect, contaminant, N-sulfo oversulfated chondroitin sulfate	468:548	Controversy remains as to the precise composition of the 2008 contaminant, and new information suggests that heparin may now be subject to adulteration with a new, difficult to detect, contaminant, N-sulfo oversulfated chondroitin sulfate.
24364596	2	12	theme	new	470:472	arg1	sulfate					542:548	a new, difficult to detect, contaminant, N-sulfo oversulfated chondroitin sulfate	468:548	a new, difficult to detect, contaminant, N-sulfo oversulfated chondroitin sulfate	468:548	Controversy remains as to the precise composition of the 2008 contaminant, and new information suggests that heparin may now be subject to adulteration with a new, difficult to detect, contaminant, N-sulfo oversulfated chondroitin sulfate.
24364596	2	13	theme	chondroitin	530:540	arg1	sulfate					542:548	a new, difficult to detect, contaminant, N-sulfo oversulfated chondroitin sulfate	468:548	a new, difficult to detect, contaminant, N-sulfo oversulfated chondroitin sulfate	468:548	Controversy remains as to the precise composition of the 2008 contaminant, and new information suggests that heparin may now be subject to adulteration with a new, difficult to detect, contaminant, N-sulfo oversulfated chondroitin sulfate.
24364596	3	14	dep	use	623:625	arg1	detect					710:715	detect	710:715	to detect N-sulfo oversulfated chondroitin sulfate	707:756	This study synthesizes this new potential contaminant and describes the use of radical depolymerization followed by liquid chromatography-mass spectrometry to detect N-sulfo oversulfated chondroitin sulfate and to confirm the structure of the 2008 contaminant as oversulfated chondroitin sulfate and not oversulfated heparan sulfate.
24364596	3	14	dep	use	623:625	arg1	confirm					765:771	confirm	765:771	to confirm the structure of the 2008 contaminant as oversulfated chondroitin sulfate and not oversulfated heparan sulfate	762:882	This study synthesizes this new potential contaminant and describes the use of radical depolymerization followed by liquid chromatography-mass spectrometry to detect N-sulfo oversulfated chondroitin sulfate and to confirm the structure of the 2008 contaminant as oversulfated chondroitin sulfate and not oversulfated heparan sulfate.
24364596	3	15	theme	radical	630:636	arg1	depolymerization					638:653	radical depolymerization	630:653	radical depolymerization followed by liquid chromatography-mass spectrometry	630:705	This study synthesizes this new potential contaminant and describes the use of radical depolymerization followed by liquid chromatography-mass spectrometry to detect N-sulfo oversulfated chondroitin sulfate and to confirm the structure of the 2008 contaminant as oversulfated chondroitin sulfate and not oversulfated heparan sulfate.
24364596	0	16	theme	radical	47:53	arg1	depolymerization					55:70	radical depolymerization	47:70	radical depolymerization	47:70	Method to detect contaminants in heparin using radical depolymerization and liquid chromatography-mass spectrometry.
24364596	3	17	theme	oversulfated	814:825	arg1	sulfate					839:845	oversulfated chondroitin sulfate	814:845	oversulfated chondroitin sulfate	814:845	This study synthesizes this new potential contaminant and describes the use of radical depolymerization followed by liquid chromatography-mass spectrometry to detect N-sulfo oversulfated chondroitin sulfate and to confirm the structure of the 2008 contaminant as oversulfated chondroitin sulfate and not oversulfated heparan sulfate.
24364596	3	18	theme	depolymerization	638:653	arg1	use					623:625	the use	619:625	the use of radical depolymerization followed by liquid chromatography-mass spectrometry to detect N-sulfo oversulfated chondroitin sulfate and to confirm the structure of the 2008 contaminant as oversulfated chondroitin sulfate and not oversulfated heparan sulfate	619:882	This study synthesizes this new potential contaminant and describes the use of radical depolymerization followed by liquid chromatography-mass spectrometry to detect N-sulfo oversulfated chondroitin sulfate and to confirm the structure of the 2008 contaminant as oversulfated chondroitin sulfate and not oversulfated heparan sulfate.
24364596	3	19	theme	new	579:581	arg1	contaminant					593:603	this new potential contaminant	574:603	this new potential contaminant	574:603	This study synthesizes this new potential contaminant and describes the use of radical depolymerization followed by liquid chromatography-mass spectrometry to detect N-sulfo oversulfated chondroitin sulfate and to confirm the structure of the 2008 contaminant as oversulfated chondroitin sulfate and not oversulfated heparan sulfate.
24364596	3	20	theme	oversulfated	725:736	arg1	sulfate					750:756	N-sulfo oversulfated chondroitin sulfate	717:756	N-sulfo oversulfated chondroitin sulfate	717:756	This study synthesizes this new potential contaminant and describes the use of radical depolymerization followed by liquid chromatography-mass spectrometry to detect N-sulfo oversulfated chondroitin sulfate and to confirm the structure of the 2008 contaminant as oversulfated chondroitin sulfate and not oversulfated heparan sulfate.
24364596	3	21	theme	potential	583:591	arg1	contaminant					593:603	this new potential contaminant	574:603	this new potential contaminant	574:603	This study synthesizes this new potential contaminant and describes the use of radical depolymerization followed by liquid chromatography-mass spectrometry to detect N-sulfo oversulfated chondroitin sulfate and to confirm the structure of the 2008 contaminant as oversulfated chondroitin sulfate and not oversulfated heparan sulfate.
24364596	2	22	theme	precise	341:347	arg1	composition					349:359	the precise composition	337:359	the precise composition of the 2008 contaminant	337:383	Controversy remains as to the precise composition of the 2008 contaminant, and new information suggests that heparin may now be subject to adulteration with a new, difficult to detect, contaminant, N-sulfo oversulfated chondroitin sulfate.
24364596	3	23	theme	contaminant	799:809	arg1	structure					777:785	the structure	773:785	the structure of the 2008 contaminant	773:809	This study synthesizes this new potential contaminant and describes the use of radical depolymerization followed by liquid chromatography-mass spectrometry to detect N-sulfo oversulfated chondroitin sulfate and to confirm the structure of the 2008 contaminant as oversulfated chondroitin sulfate and not oversulfated heparan sulfate.
24364596	1	24	theme	important	141:149	arg1	Heparin					117:123	Heparin	117:123	Heparin	117:123	Heparin is a critically important anticoagulant drug that was contaminated with a persulfonated polysaccharide in 2008, resulting in a number of severe adverse reactions, some leading to death.
24364596	1	24	theme	important	141:149	arg1	drug					165:168	a critically important anticoagulant drug	128:168	a critically important anticoagulant drug that was contaminated with a persulfonated polysaccharide in 2008, resulting in a number of severe adverse reactions, some leading to death	128:308	Heparin is a critically important anticoagulant drug that was contaminated with a persulfonated polysaccharide in 2008, resulting in a number of severe adverse reactions, some leading to death.
24364596	3	25	theme	liquid	667:672	arg1	spectrometry					694:705	liquid chromatography-mass spectrometry	667:705	liquid chromatography-mass spectrometry	667:705	This study synthesizes this new potential contaminant and describes the use of radical depolymerization followed by liquid chromatography-mass spectrometry to detect N-sulfo oversulfated chondroitin sulfate and to confirm the structure of the 2008 contaminant as oversulfated chondroitin sulfate and not oversulfated heparan sulfate.
24364596	1	26	theme	anticoagulant	151:163	arg1	Heparin					117:123	Heparin	117:123	Heparin	117:123	Heparin is a critically important anticoagulant drug that was contaminated with a persulfonated polysaccharide in 2008, resulting in a number of severe adverse reactions, some leading to death.
24364596	1	26	theme	anticoagulant	151:163	arg1	drug					165:168	a critically important anticoagulant drug	128:168	a critically important anticoagulant drug that was contaminated with a persulfonated polysaccharide in 2008, resulting in a number of severe adverse reactions, some leading to death	128:308	Heparin is a critically important anticoagulant drug that was contaminated with a persulfonated polysaccharide in 2008, resulting in a number of severe adverse reactions, some leading to death.
24364596	3	27	theme	chromatography-mass	674:692	arg1	spectrometry					694:705	liquid chromatography-mass spectrometry	667:705	liquid chromatography-mass spectrometry	667:705	This study synthesizes this new potential contaminant and describes the use of radical depolymerization followed by liquid chromatography-mass spectrometry to detect N-sulfo oversulfated chondroitin sulfate and to confirm the structure of the 2008 contaminant as oversulfated chondroitin sulfate and not oversulfated heparan sulfate.
24364596	1	28	from	polysaccharide	213:226	arg1	2008					231:234	2008	231:234	2008	231:234	Heparin is a critically important anticoagulant drug that was contaminated with a persulfonated polysaccharide in 2008, resulting in a number of severe adverse reactions, some leading to death.
24364596	2	29	theme	contaminant	373:383	arg1	composition					349:359	the precise composition	337:359	the precise composition of the 2008 contaminant	337:383	Controversy remains as to the precise composition of the 2008 contaminant, and new information suggests that heparin may now be subject to adulteration with a new, difficult to detect, contaminant, N-sulfo oversulfated chondroitin sulfate.
24364596	3	30	theme	chondroitin	827:837	arg1	sulfate					839:845	oversulfated chondroitin sulfate	814:845	oversulfated chondroitin sulfate	814:845	This study synthesizes this new potential contaminant and describes the use of radical depolymerization followed by liquid chromatography-mass spectrometry to detect N-sulfo oversulfated chondroitin sulfate and to confirm the structure of the 2008 contaminant as oversulfated chondroitin sulfate and not oversulfated heparan sulfate.
24364596	3	31	theme	oversulfated	855:866	arg1	sulfate					876:882	not oversulfated heparan sulfate	851:882	not oversulfated heparan sulfate	851:882	This study synthesizes this new potential contaminant and describes the use of radical depolymerization followed by liquid chromatography-mass spectrometry to detect N-sulfo oversulfated chondroitin sulfate and to confirm the structure of the 2008 contaminant as oversulfated chondroitin sulfate and not oversulfated heparan sulfate.
24364596	0	32	from	contaminants	17:28	arg1	heparin					33:39	heparin	33:39	heparin using radical depolymerization and liquid chromatography-mass spectrometry	33:114	Method to detect contaminants in heparin using radical depolymerization and liquid chromatography-mass spectrometry.
25541639	6	0	theme	alginate	1242:1249	arg1	components					1251:1260	the alginate components	1238:1260	the alginate components in the composites	1238:1278	Due to the alginate components in the composites, the scaffolds showed significantly enhanced wetting behavior, water-absorption (∼12-fold), and meaningful biological activities (∼2.1-fold for cell-seeding efficiency, ∼2.5-fold for cell-viability at 7 days, ∼3.4-fold for calcium deposition), compared with a pure PCL scaffold.
25541639	1	1	theme	biomedical	153:162	arg1	applications					164:175	biomedical applications	153:175	biomedical applications	153:175	Alginates have been used widely in biomedical applications because of good biocompatibility, low cost, and rapid gelation in the presence of calcium ions.
25541639	6	2	from	days	1483:1486	arg1	cell-viability					1463:1476	cell-viability	1463:1476	cell-viability at 7 days	1463:1486	Due to the alginate components in the composites, the scaffolds showed significantly enhanced wetting behavior, water-absorption (∼12-fold), and meaningful biological activities (∼2.1-fold for cell-seeding efficiency, ∼2.5-fold for cell-viability at 7 days, ∼3.4-fold for calcium deposition), compared with a pure PCL scaffold.
25541639	3	3	theme	synthetic	519:527	arg1	poly					538:541	poly	538:541	poly(ε-caprolactone)	538:557	To overcome these shortcomings of alginates, we suggest a new composite system, consisting of a synthetic polymer, poly(ε-caprolactone), and various weight fractions (10-40 wt %) of alginate.
25541639	3	3	theme	synthetic	519:527	arg1	polymer					529:535	a synthetic polymer	517:535	a synthetic polymer	517:535	To overcome these shortcomings of alginates, we suggest a new composite system, consisting of a synthetic polymer, poly(ε-caprolactone), and various weight fractions (10-40 wt %) of alginate.
25541639	6	4	theme	cell-seeding	1424:1435	arg1	efficiency					1437:1446	cell-seeding efficiency	1424:1446	cell-seeding efficiency	1424:1446	Due to the alginate components in the composites, the scaffolds showed significantly enhanced wetting behavior, water-absorption (∼12-fold), and meaningful biological activities (∼2.1-fold for cell-seeding efficiency, ∼2.5-fold for cell-viability at 7 days, ∼3.4-fold for calcium deposition), compared with a pure PCL scaffold.
25541639	0	5	theme	cellular	97:104	arg1	activities					106:115	cellular activities	97:115	cellular activities	97:115	PCL/alginate composite scaffolds for hard tissue engineering: fabrication, characterization, and cellular activities.
25541639	3	6	theme	alginate	605:612	arg1	system					495:500	a new composite system	479:500	a new composite system	479:500	To overcome these shortcomings of alginates, we suggest a new composite system, consisting of a synthetic polymer, poly(ε-caprolactone), and various weight fractions (10-40 wt %) of alginate.
25541639	3	6	theme	alginate	605:612	arg1	fractions					579:587	various weight fractions	564:587	various weight fractions (10-40 wt %) of alginate	564:612	To overcome these shortcomings of alginates, we suggest a new composite system, consisting of a synthetic polymer, poly(ε-caprolactone), and various weight fractions (10-40 wt %) of alginate.
25541639	3	6	theme	alginate	605:612	arg1	%					599:599	10-40 wt %	590:599	10-40 wt %	590:599	To overcome these shortcomings of alginates, we suggest a new composite system, consisting of a synthetic polymer, poly(ε-caprolactone), and various weight fractions (10-40 wt %) of alginate.
25541639	4	7	theme	100	752:754	arg1	%					755:755	%	755:755	%	755:755	The fabricated composite scaffolds displayed a multilayered 3D structure, consisting of microsized composite struts, and they provided a 100% offset for each layer.
25541639	3	8	theme	various	564:570	arg1	fractions					579:587	various weight fractions	564:587	various weight fractions (10-40 wt %) of alginate	564:612	To overcome these shortcomings of alginates, we suggest a new composite system, consisting of a synthetic polymer, poly(ε-caprolactone), and various weight fractions (10-40 wt %) of alginate.
25541639	3	8	theme	various	564:570	arg1	%					599:599	10-40 wt %	590:599	10-40 wt %	590:599	To overcome these shortcomings of alginates, we suggest a new composite system, consisting of a synthetic polymer, poly(ε-caprolactone), and various weight fractions (10-40 wt %) of alginate.
25541639	5	9	dep	only	903:906	arg1	not					899:901	not	899:901	not	899:901	To show the feasibility of the scaffold for hard tissue regeneration, the composite scaffolds fabricated were assessed not only for physical properties, including surface roughness, tensile strength, and water absorption and wetting, but also in vitro osteoblastic cellular responses (cell-seeding efficiency, cell viability, fluorescence analyses, alkaline phosphatase (ALP) activity, and mineralization) by culturing with preosteoblasts (MC3T3-E1).
25541639	5	10	theme	surface	943:949	arg1	roughness					951:959	surface roughness	943:959	surface roughness	943:959	To show the feasibility of the scaffold for hard tissue regeneration, the composite scaffolds fabricated were assessed not only for physical properties, including surface roughness, tensile strength, and water absorption and wetting, but also in vitro osteoblastic cellular responses (cell-seeding efficiency, cell viability, fluorescence analyses, alkaline phosphatase (ALP) activity, and mineralization) by culturing with preosteoblasts (MC3T3-E1).
25541639	1	11	theme	calcium	259:265	arg1	ions					267:270	calcium ions	259:270	calcium ions	259:270	Alginates have been used widely in biomedical applications because of good biocompatibility, low cost, and rapid gelation in the presence of calcium ions.
25541639	5	12	theme	cell-seeding	1065:1076	arg1	efficiency					1078:1087	cell-seeding efficiency	1065:1087	cell-seeding efficiency	1065:1087	To show the feasibility of the scaffold for hard tissue regeneration, the composite scaffolds fabricated were assessed not only for physical properties, including surface roughness, tensile strength, and water absorption and wetting, but also in vitro osteoblastic cellular responses (cell-seeding efficiency, cell viability, fluorescence analyses, alkaline phosphatase (ALP) activity, and mineralization) by culturing with preosteoblasts (MC3T3-E1).
25541639	3	13	theme	composite	485:493	arg1	system					495:500	a new composite system	479:500	a new composite system	479:500	To overcome these shortcomings of alginates, we suggest a new composite system, consisting of a synthetic polymer, poly(ε-caprolactone), and various weight fractions (10-40 wt %) of alginate.
25541639	1	14	theme	good	188:191	arg1	biocompatibility					193:208	good biocompatibility	188:208	good biocompatibility	188:208	Alginates have been used widely in biomedical applications because of good biocompatibility, low cost, and rapid gelation in the presence of calcium ions.
25541639	5	15	theme	hard	824:827	arg1	regeneration					836:847	hard tissue regeneration	824:847	hard tissue regeneration	824:847	To show the feasibility of the scaffold for hard tissue regeneration, the composite scaffolds fabricated were assessed not only for physical properties, including surface roughness, tensile strength, and water absorption and wetting, but also in vitro osteoblastic cellular responses (cell-seeding efficiency, cell viability, fluorescence analyses, alkaline phosphatase (ALP) activity, and mineralization) by culturing with preosteoblasts (MC3T3-E1).
25541639	6	16	theme	meaningful	1376:1385	arg1	activities					1398:1407	meaningful biological activities	1376:1407	meaningful biological activities (∼2.1-fold for cell-seeding efficiency, ∼2.5-fold for cell-viability at 7 days, ∼3.4-fold for calcium deposition)	1376:1521	Due to the alginate components in the composites, the scaffolds showed significantly enhanced wetting behavior, water-absorption (∼12-fold), and meaningful biological activities (∼2.1-fold for cell-seeding efficiency, ∼2.5-fold for cell-viability at 7 days, ∼3.4-fold for calcium deposition), compared with a pure PCL scaffold.
25541639	5	17	dep	properties	921:930	arg1	including					933:941	including	933:941	including surface roughness, tensile strength, and water absorption	933:999	To show the feasibility of the scaffold for hard tissue regeneration, the composite scaffolds fabricated were assessed not only for physical properties, including surface roughness, tensile strength, and water absorption and wetting, but also in vitro osteoblastic cellular responses (cell-seeding efficiency, cell viability, fluorescence analyses, alkaline phosphatase (ALP) activity, and mineralization) by culturing with preosteoblasts (MC3T3-E1).
25541639	5	17	dep	properties	921:930	arg1	wetting					1005:1011	wetting	1005:1011	wetting	1005:1011	To show the feasibility of the scaffold for hard tissue regeneration, the composite scaffolds fabricated were assessed not only for physical properties, including surface roughness, tensile strength, and water absorption and wetting, but also in vitro osteoblastic cellular responses (cell-seeding efficiency, cell viability, fluorescence analyses, alkaline phosphatase (ALP) activity, and mineralization) by culturing with preosteoblasts (MC3T3-E1).
25541639	4	18	theme	%	755:755	arg1	offset					757:762	a 100% offset	750:762	a 100% offset for each layer	750:777	The fabricated composite scaffolds displayed a multilayered 3D structure, consisting of microsized composite struts, and they provided a 100% offset for each layer.
25541639	6	19	theme	calcium	1503:1509	arg1	deposition					1511:1520	calcium deposition	1503:1520	calcium deposition	1503:1520	Due to the alginate components in the composites, the scaffolds showed significantly enhanced wetting behavior, water-absorption (∼12-fold), and meaningful biological activities (∼2.1-fold for cell-seeding efficiency, ∼2.5-fold for cell-viability at 7 days, ∼3.4-fold for calcium deposition), compared with a pure PCL scaffold.
25541639	5	20	theme	tissue	829:834	arg1	regeneration					836:847	hard tissue regeneration	824:847	hard tissue regeneration	824:847	To show the feasibility of the scaffold for hard tissue regeneration, the composite scaffolds fabricated were assessed not only for physical properties, including surface roughness, tensile strength, and water absorption and wetting, but also in vitro osteoblastic cellular responses (cell-seeding efficiency, cell viability, fluorescence analyses, alkaline phosphatase (ALP) activity, and mineralization) by culturing with preosteoblasts (MC3T3-E1).
25541639	0	21	theme	composite	13:21	arg1	scaffolds					23:31	PCL/alginate composite scaffolds	0:31	PCL/alginate composite scaffolds for hard tissue engineering: fabrication, characterization, and cellular activities.	0:116	PCL/alginate composite scaffolds for hard tissue engineering: fabrication, characterization, and cellular activities.
25541639	0	21	theme	composite	13:21	arg1	characterization					75:90	characterization	75:90	characterization	75:90	PCL/alginate composite scaffolds for hard tissue engineering: fabrication, characterization, and cellular activities.
25541639	0	21	theme	composite	13:21	arg1	fabrication					62:72	fabrication	62:72	fabrication	62:72	PCL/alginate composite scaffolds for hard tissue engineering: fabrication, characterization, and cellular activities.
25541639	1	22	used	used	138:141	arg2	Alginates					118:126	Alginates	118:126	Alginates	118:126	Alginates have been used widely in biomedical applications because of good biocompatibility, low cost, and rapid gelation in the presence of calcium ions.
25541639	2	23	theme	engineering	397:407	arg1	applications					409:420	hard-tissue engineering applications	385:420	hard-tissue engineering applications	385:420	However, poor mechanical properties and fabrication-ability for three-dimensional shapes have been obstacles in hard-tissue engineering applications.
25541639	0	24	theme	PCL/alginate	0:11	arg1	scaffolds					23:31	PCL/alginate composite scaffolds	0:31	PCL/alginate composite scaffolds for hard tissue engineering: fabrication, characterization, and cellular activities.	0:116	PCL/alginate composite scaffolds for hard tissue engineering: fabrication, characterization, and cellular activities.
25541639	0	24	theme	PCL/alginate	0:11	arg1	characterization					75:90	characterization	75:90	characterization	75:90	PCL/alginate composite scaffolds for hard tissue engineering: fabrication, characterization, and cellular activities.
25541639	0	24	theme	PCL/alginate	0:11	arg1	fabrication					62:72	fabrication	62:72	fabrication	62:72	PCL/alginate composite scaffolds for hard tissue engineering: fabrication, characterization, and cellular activities.
25541639	1	25	theme	ions	267:270	arg1	presence					247:254	the presence	243:254	the presence of calcium ions	243:270	Alginates have been used widely in biomedical applications because of good biocompatibility, low cost, and rapid gelation in the presence of calcium ions.
25541639	3	26	theme	weight	572:577	arg1	fractions					579:587	various weight fractions	564:587	various weight fractions (10-40 wt %) of alginate	564:612	To overcome these shortcomings of alginates, we suggest a new composite system, consisting of a synthetic polymer, poly(ε-caprolactone), and various weight fractions (10-40 wt %) of alginate.
25541639	3	26	theme	weight	572:577	arg1	%					599:599	10-40 wt %	590:599	10-40 wt %	590:599	To overcome these shortcomings of alginates, we suggest a new composite system, consisting of a synthetic polymer, poly(ε-caprolactone), and various weight fractions (10-40 wt %) of alginate.
25541639	2	27	theme	hard-tissue	385:395	arg1	applications					409:420	hard-tissue engineering applications	385:420	hard-tissue engineering applications	385:420	However, poor mechanical properties and fabrication-ability for three-dimensional shapes have been obstacles in hard-tissue engineering applications.
25541639	5	28	theme	ALP	1151:1153	arg1	activity					1156:1163	alkaline phosphatase (ALP) activity	1129:1163	alkaline phosphatase (ALP) activity	1129:1163	To show the feasibility of the scaffold for hard tissue regeneration, the composite scaffolds fabricated were assessed not only for physical properties, including surface roughness, tensile strength, and water absorption and wetting, but also in vitro osteoblastic cellular responses (cell-seeding efficiency, cell viability, fluorescence analyses, alkaline phosphatase (ALP) activity, and mineralization) by culturing with preosteoblasts (MC3T3-E1).
25541639	2	29	from	obstacles	372:380	arg1	applications					409:420	hard-tissue engineering applications	385:420	hard-tissue engineering applications	385:420	However, poor mechanical properties and fabrication-ability for three-dimensional shapes have been obstacles in hard-tissue engineering applications.
25541639	2	30	theme	mechanical	287:296	arg1	properties					298:307	poor mechanical properties	282:307	poor mechanical properties	282:307	However, poor mechanical properties and fabrication-ability for three-dimensional shapes have been obstacles in hard-tissue engineering applications.
25541639	3	31	theme	new	481:483	arg1	system					495:500	a new composite system	479:500	a new composite system	479:500	To overcome these shortcomings of alginates, we suggest a new composite system, consisting of a synthetic polymer, poly(ε-caprolactone), and various weight fractions (10-40 wt %) of alginate.
25541639	5	32	theme	physical	912:919	arg1	properties					921:930	physical properties	912:930	physical properties	912:930	To show the feasibility of the scaffold for hard tissue regeneration, the composite scaffolds fabricated were assessed not only for physical properties, including surface roughness, tensile strength, and water absorption and wetting, but also in vitro osteoblastic cellular responses (cell-seeding efficiency, cell viability, fluorescence analyses, alkaline phosphatase (ALP) activity, and mineralization) by culturing with preosteoblasts (MC3T3-E1).
25541639	5	33	theme	vitro	1026:1030	arg1	responses					1054:1062	vitro osteoblastic cellular responses	1026:1062	vitro osteoblastic cellular responses	1026:1062	To show the feasibility of the scaffold for hard tissue regeneration, the composite scaffolds fabricated were assessed not only for physical properties, including surface roughness, tensile strength, and water absorption and wetting, but also in vitro osteoblastic cellular responses (cell-seeding efficiency, cell viability, fluorescence analyses, alkaline phosphatase (ALP) activity, and mineralization) by culturing with preosteoblasts (MC3T3-E1).
25541639	2	34	theme	poor	282:285	arg1	properties					298:307	poor mechanical properties	282:307	poor mechanical properties	282:307	However, poor mechanical properties and fabrication-ability for three-dimensional shapes have been obstacles in hard-tissue engineering applications.
25541639	1	35	theme	low	211:213	arg1	cost					215:218	low cost	211:218	low cost	211:218	Alginates have been used widely in biomedical applications because of good biocompatibility, low cost, and rapid gelation in the presence of calcium ions.
25541639	0	36	theme	tissue	42:47	arg1	engineering					49:59	hard tissue engineering	37:59	hard tissue engineering	37:59	PCL/alginate composite scaffolds for hard tissue engineering: fabrication, characterization, and cellular activities.
25541639	4	37	theme	multilayered	662:673	arg1	structure					678:686	a multilayered 3D structure	660:686	a multilayered 3D structure	660:686	The fabricated composite scaffolds displayed a multilayered 3D structure, consisting of microsized composite struts, and they provided a 100% offset for each layer.
25541639	5	38	theme	scaffold	811:818	arg1	feasibility					792:802	the feasibility	788:802	the feasibility of the scaffold for hard tissue regeneration	788:847	To show the feasibility of the scaffold for hard tissue regeneration, the composite scaffolds fabricated were assessed not only for physical properties, including surface roughness, tensile strength, and water absorption and wetting, but also in vitro osteoblastic cellular responses (cell-seeding efficiency, cell viability, fluorescence analyses, alkaline phosphatase (ALP) activity, and mineralization) by culturing with preosteoblasts (MC3T3-E1).
25541639	5	39	theme	water	984:988	arg1	absorption					990:999	water absorption	984:999	water absorption	984:999	To show the feasibility of the scaffold for hard tissue regeneration, the composite scaffolds fabricated were assessed not only for physical properties, including surface roughness, tensile strength, and water absorption and wetting, but also in vitro osteoblastic cellular responses (cell-seeding efficiency, cell viability, fluorescence analyses, alkaline phosphatase (ALP) activity, and mineralization) by culturing with preosteoblasts (MC3T3-E1).
25541639	3	40	theme	alginates	457:465	arg1	shortcomings					441:452	these shortcomings	435:452	these shortcomings of alginates	435:465	To overcome these shortcomings of alginates, we suggest a new composite system, consisting of a synthetic polymer, poly(ε-caprolactone), and various weight fractions (10-40 wt %) of alginate.
25541639	4	41	theme	composite	630:638	arg1	scaffolds					640:648	The fabricated composite scaffolds	615:648	The fabricated composite scaffolds	615:648	The fabricated composite scaffolds displayed a multilayered 3D structure, consisting of microsized composite struts, and they provided a 100% offset for each layer.
25541639	0	42	theme	hard	37:40	arg1	engineering					49:59	hard tissue engineering	37:59	hard tissue engineering	37:59	PCL/alginate composite scaffolds for hard tissue engineering: fabrication, characterization, and cellular activities.
25541639	5	43	theme	cellular	1045:1052	arg1	responses					1054:1062	vitro osteoblastic cellular responses	1026:1062	vitro osteoblastic cellular responses	1026:1062	To show the feasibility of the scaffold for hard tissue regeneration, the composite scaffolds fabricated were assessed not only for physical properties, including surface roughness, tensile strength, and water absorption and wetting, but also in vitro osteoblastic cellular responses (cell-seeding efficiency, cell viability, fluorescence analyses, alkaline phosphatase (ALP) activity, and mineralization) by culturing with preosteoblasts (MC3T3-E1).
25541639	3	44	theme	wt	596:597	arg1	fractions					579:587	various weight fractions	564:587	various weight fractions (10-40 wt %) of alginate	564:612	To overcome these shortcomings of alginates, we suggest a new composite system, consisting of a synthetic polymer, poly(ε-caprolactone), and various weight fractions (10-40 wt %) of alginate.
25541639	3	44	theme	wt	596:597	arg1	%					599:599	10-40 wt %	590:599	10-40 wt %	590:599	To overcome these shortcomings of alginates, we suggest a new composite system, consisting of a synthetic polymer, poly(ε-caprolactone), and various weight fractions (10-40 wt %) of alginate.
25541639	4	45	theme	fabricated	619:628	arg1	scaffolds					640:648	The fabricated composite scaffolds	615:648	The fabricated composite scaffolds	615:648	The fabricated composite scaffolds displayed a multilayered 3D structure, consisting of microsized composite struts, and they provided a 100% offset for each layer.
25541639	6	46	dep	showed	1295:1300	arg1	enhanced					1316:1323	enhanced	1316:1323	enhanced wetting behavior, water-absorption (∼12-fold), and meaningful biological activities (∼2.1-fold for cell-seeding efficiency, ∼2.5-fold for cell-viability at 7 days, ∼3.4-fold for calcium deposition)	1316:1521	Due to the alginate components in the composites, the scaffolds showed significantly enhanced wetting behavior, water-absorption (∼12-fold), and meaningful biological activities (∼2.1-fold for cell-seeding efficiency, ∼2.5-fold for cell-viability at 7 days, ∼3.4-fold for calcium deposition), compared with a pure PCL scaffold.
25541639	6	47	from	components	1251:1260	arg1	composites					1269:1278	the composites	1265:1278	the composites	1265:1278	Due to the alginate components in the composites, the scaffolds showed significantly enhanced wetting behavior, water-absorption (∼12-fold), and meaningful biological activities (∼2.1-fold for cell-seeding efficiency, ∼2.5-fold for cell-viability at 7 days, ∼3.4-fold for calcium deposition), compared with a pure PCL scaffold.
25541639	6	48	dep	activities	1398:1407	arg1	∼2.1-fold					1410:1418	∼2.1-fold	1410:1418	∼2.1-fold	1410:1418	Due to the alginate components in the composites, the scaffolds showed significantly enhanced wetting behavior, water-absorption (∼12-fold), and meaningful biological activities (∼2.1-fold for cell-seeding efficiency, ∼2.5-fold for cell-viability at 7 days, ∼3.4-fold for calcium deposition), compared with a pure PCL scaffold.
25541639	4	49	theme	microsized	703:712	arg1	struts					724:729	microsized composite struts	703:729	microsized composite struts	703:729	The fabricated composite scaffolds displayed a multilayered 3D structure, consisting of microsized composite struts, and they provided a 100% offset for each layer.
25541639	5	50	theme	fluorescence	1106:1117	arg1	analyses					1119:1126	fluorescence analyses	1106:1126	fluorescence analyses	1106:1126	To show the feasibility of the scaffold for hard tissue regeneration, the composite scaffolds fabricated were assessed not only for physical properties, including surface roughness, tensile strength, and water absorption and wetting, but also in vitro osteoblastic cellular responses (cell-seeding efficiency, cell viability, fluorescence analyses, alkaline phosphatase (ALP) activity, and mineralization) by culturing with preosteoblasts (MC3T3-E1).
25541639	5	51	theme	alkaline	1129:1136	arg1	activity					1156:1163	alkaline phosphatase (ALP) activity	1129:1163	alkaline phosphatase (ALP) activity	1129:1163	To show the feasibility of the scaffold for hard tissue regeneration, the composite scaffolds fabricated were assessed not only for physical properties, including surface roughness, tensile strength, and water absorption and wetting, but also in vitro osteoblastic cellular responses (cell-seeding efficiency, cell viability, fluorescence analyses, alkaline phosphatase (ALP) activity, and mineralization) by culturing with preosteoblasts (MC3T3-E1).
25541639	6	52	theme	PCL	1545:1547	arg1	scaffold					1549:1556	a pure PCL scaffold	1538:1556	a pure PCL scaffold	1538:1556	Due to the alginate components in the composites, the scaffolds showed significantly enhanced wetting behavior, water-absorption (∼12-fold), and meaningful biological activities (∼2.1-fold for cell-seeding efficiency, ∼2.5-fold for cell-viability at 7 days, ∼3.4-fold for calcium deposition), compared with a pure PCL scaffold.
25541639	2	53	theme	three-dimensional	337:353	arg1	shapes					355:360	three-dimensional shapes	337:360	three-dimensional shapes	337:360	However, poor mechanical properties and fabrication-ability for three-dimensional shapes have been obstacles in hard-tissue engineering applications.
25541639	5	54	theme	phosphatase	1138:1148	arg1	activity					1156:1163	alkaline phosphatase (ALP) activity	1129:1163	alkaline phosphatase (ALP) activity	1129:1163	To show the feasibility of the scaffold for hard tissue regeneration, the composite scaffolds fabricated were assessed not only for physical properties, including surface roughness, tensile strength, and water absorption and wetting, but also in vitro osteoblastic cellular responses (cell-seeding efficiency, cell viability, fluorescence analyses, alkaline phosphatase (ALP) activity, and mineralization) by culturing with preosteoblasts (MC3T3-E1).
25541639	6	55	dep	∼2.1-fold	1410:1418	arg1	∼3.4-fold					1489:1497	∼3.4-fold	1489:1497	∼3.4-fold	1489:1497	Due to the alginate components in the composites, the scaffolds showed significantly enhanced wetting behavior, water-absorption (∼12-fold), and meaningful biological activities (∼2.1-fold for cell-seeding efficiency, ∼2.5-fold for cell-viability at 7 days, ∼3.4-fold for calcium deposition), compared with a pure PCL scaffold.
25541639	6	55	dep	∼2.1-fold	1410:1418	arg1	∼2.5-fold					1449:1457	∼2.5-fold	1449:1457	∼2.5-fold	1449:1457	Due to the alginate components in the composites, the scaffolds showed significantly enhanced wetting behavior, water-absorption (∼12-fold), and meaningful biological activities (∼2.1-fold for cell-seeding efficiency, ∼2.5-fold for cell-viability at 7 days, ∼3.4-fold for calcium deposition), compared with a pure PCL scaffold.
25541639	5	56	theme	tensile	962:968	arg1	strength					970:977	tensile strength	962:977	tensile strength	962:977	To show the feasibility of the scaffold for hard tissue regeneration, the composite scaffolds fabricated were assessed not only for physical properties, including surface roughness, tensile strength, and water absorption and wetting, but also in vitro osteoblastic cellular responses (cell-seeding efficiency, cell viability, fluorescence analyses, alkaline phosphatase (ALP) activity, and mineralization) by culturing with preosteoblasts (MC3T3-E1).
25541639	6	57	theme	pure	1540:1543	arg1	scaffold					1549:1556	a pure PCL scaffold	1538:1556	a pure PCL scaffold	1538:1556	Due to the alginate components in the composites, the scaffolds showed significantly enhanced wetting behavior, water-absorption (∼12-fold), and meaningful biological activities (∼2.1-fold for cell-seeding efficiency, ∼2.5-fold for cell-viability at 7 days, ∼3.4-fold for calcium deposition), compared with a pure PCL scaffold.
25541639	5	58	dep	assessed	890:897	arg1	efficiency					1078:1087	cell-seeding efficiency	1065:1087	cell-seeding efficiency	1065:1087	To show the feasibility of the scaffold for hard tissue regeneration, the composite scaffolds fabricated were assessed not only for physical properties, including surface roughness, tensile strength, and water absorption and wetting, but also in vitro osteoblastic cellular responses (cell-seeding efficiency, cell viability, fluorescence analyses, alkaline phosphatase (ALP) activity, and mineralization) by culturing with preosteoblasts (MC3T3-E1).
25541639	5	58	dep	assessed	890:897	arg1	viability					1095:1103	cell viability	1090:1103	cell viability	1090:1103	To show the feasibility of the scaffold for hard tissue regeneration, the composite scaffolds fabricated were assessed not only for physical properties, including surface roughness, tensile strength, and water absorption and wetting, but also in vitro osteoblastic cellular responses (cell-seeding efficiency, cell viability, fluorescence analyses, alkaline phosphatase (ALP) activity, and mineralization) by culturing with preosteoblasts (MC3T3-E1).
25541639	5	58	dep	assessed	890:897	arg1	analyses					1119:1126	fluorescence analyses	1106:1126	fluorescence analyses	1106:1126	To show the feasibility of the scaffold for hard tissue regeneration, the composite scaffolds fabricated were assessed not only for physical properties, including surface roughness, tensile strength, and water absorption and wetting, but also in vitro osteoblastic cellular responses (cell-seeding efficiency, cell viability, fluorescence analyses, alkaline phosphatase (ALP) activity, and mineralization) by culturing with preosteoblasts (MC3T3-E1).
25541639	5	58	dep	assessed	890:897	arg1	mineralization					1170:1183	mineralization	1170:1183	mineralization	1170:1183	To show the feasibility of the scaffold for hard tissue regeneration, the composite scaffolds fabricated were assessed not only for physical properties, including surface roughness, tensile strength, and water absorption and wetting, but also in vitro osteoblastic cellular responses (cell-seeding efficiency, cell viability, fluorescence analyses, alkaline phosphatase (ALP) activity, and mineralization) by culturing with preosteoblasts (MC3T3-E1).
25541639	5	58	dep	assessed	890:897	arg1	activity					1156:1163	alkaline phosphatase (ALP) activity	1129:1163	alkaline phosphatase (ALP) activity	1129:1163	To show the feasibility of the scaffold for hard tissue regeneration, the composite scaffolds fabricated were assessed not only for physical properties, including surface roughness, tensile strength, and water absorption and wetting, but also in vitro osteoblastic cellular responses (cell-seeding efficiency, cell viability, fluorescence analyses, alkaline phosphatase (ALP) activity, and mineralization) by culturing with preosteoblasts (MC3T3-E1).
25541639	0	59	dep	scaffolds	23:31	arg1	characterization					75:90	characterization	75:90	characterization	75:90	PCL/alginate composite scaffolds for hard tissue engineering: fabrication, characterization, and cellular activities.
25541639	0	59	dep	scaffolds	23:31	arg1	scaffolds					23:31	PCL/alginate composite scaffolds	0:31	PCL/alginate composite scaffolds for hard tissue engineering: fabrication, characterization, and cellular activities.	0:116	PCL/alginate composite scaffolds for hard tissue engineering: fabrication, characterization, and cellular activities.
25541639	0	59	dep	scaffolds	23:31	arg1	fabrication					62:72	fabrication	62:72	fabrication	62:72	PCL/alginate composite scaffolds for hard tissue engineering: fabrication, characterization, and cellular activities.
25541639	0	59	dep	scaffolds	23:31	arg1	activities					106:115	cellular activities	97:115	cellular activities	97:115	PCL/alginate composite scaffolds for hard tissue engineering: fabrication, characterization, and cellular activities.
25541639	5	60	theme	osteoblastic	1032:1043	arg1	responses					1054:1062	vitro osteoblastic cellular responses	1026:1062	vitro osteoblastic cellular responses	1026:1062	To show the feasibility of the scaffold for hard tissue regeneration, the composite scaffolds fabricated were assessed not only for physical properties, including surface roughness, tensile strength, and water absorption and wetting, but also in vitro osteoblastic cellular responses (cell-seeding efficiency, cell viability, fluorescence analyses, alkaline phosphatase (ALP) activity, and mineralization) by culturing with preosteoblasts (MC3T3-E1).
25541639	4	61	theme	composite	714:722	arg1	struts					724:729	microsized composite struts	703:729	microsized composite struts	703:729	The fabricated composite scaffolds displayed a multilayered 3D structure, consisting of microsized composite struts, and they provided a 100% offset for each layer.
25541639	1	62	theme	rapid	225:229	arg1	gelation					231:238	rapid gelation	225:238	rapid gelation	225:238	Alginates have been used widely in biomedical applications because of good biocompatibility, low cost, and rapid gelation in the presence of calcium ions.
25541639	5	63	theme	composite	854:862	arg1	scaffolds					864:872	the composite scaffolds	850:872	the composite scaffolds fabricated	850:883	To show the feasibility of the scaffold for hard tissue regeneration, the composite scaffolds fabricated were assessed not only for physical properties, including surface roughness, tensile strength, and water absorption and wetting, but also in vitro osteoblastic cellular responses (cell-seeding efficiency, cell viability, fluorescence analyses, alkaline phosphatase (ALP) activity, and mineralization) by culturing with preosteoblasts (MC3T3-E1).
25541639	5	64	theme	cell	1090:1093	arg1	viability					1095:1103	cell viability	1090:1103	cell viability	1090:1103	To show the feasibility of the scaffold for hard tissue regeneration, the composite scaffolds fabricated were assessed not only for physical properties, including surface roughness, tensile strength, and water absorption and wetting, but also in vitro osteoblastic cellular responses (cell-seeding efficiency, cell viability, fluorescence analyses, alkaline phosphatase (ALP) activity, and mineralization) by culturing with preosteoblasts (MC3T3-E1).
25541639	6	65	theme	biological	1387:1396	arg1	activities					1398:1407	meaningful biological activities	1376:1407	meaningful biological activities (∼2.1-fold for cell-seeding efficiency, ∼2.5-fold for cell-viability at 7 days, ∼3.4-fold for calcium deposition)	1376:1521	Due to the alginate components in the composites, the scaffolds showed significantly enhanced wetting behavior, water-absorption (∼12-fold), and meaningful biological activities (∼2.1-fold for cell-seeding efficiency, ∼2.5-fold for cell-viability at 7 days, ∼3.4-fold for calcium deposition), compared with a pure PCL scaffold.
25541639	4	66	theme	3D	675:676	arg1	structure					678:686	a multilayered 3D structure	660:686	a multilayered 3D structure	660:686	The fabricated composite scaffolds displayed a multilayered 3D structure, consisting of microsized composite struts, and they provided a 100% offset for each layer.
27750379	4	0	theme	L.	472:473	arg1	pectin					475:480	Heracleum L. pectin	462:480	Heracleum L. pectin (HP)	462:485	For cryogel preparation, apple pectin (AP), Heracleum L. pectin (HP), and chitosan samples with different physical and chemical characteristics were used.
27750379	4	0	theme	L.	472:473	arg1	HP					483:484	HP	483:484	HP	483:484	For cryogel preparation, apple pectin (AP), Heracleum L. pectin (HP), and chitosan samples with different physical and chemical characteristics were used.
27750379	10	1	theme	cytotoxicity	1291:1302	arg1	data					1304:1307	light cytotoxicity data	1285:1307	light cytotoxicity data as well as a low level of hemolysis of composite cryogels based on AP and chitosan	1285:1390	Based on the results of low immunogenicity, light cytotoxicity data as well as a low level of hemolysis of composite cryogels based on AP and chitosan are biocompatible and can potentially be used in biomedicine.
27750379	4	2	theme	chemical	537:544	arg1	characteristics					546:560	different physical and chemical characteristics	514:560	different physical and chemical characteristics	514:560	For cryogel preparation, apple pectin (AP), Heracleum L. pectin (HP), and chitosan samples with different physical and chemical characteristics were used.
27750379	0	3	theme	biomedical	80:89	arg1	application					91:101	biomedical application	80:101	biomedical application	80:101	Preparation and biocompatibility evaluation of pectin and chitosan cryogels for biomedical application.
27750379	12	4	dep	Res	1500:1502	arg1	105A					1512:1515	105A	1512:1515	105A	1512:1515	J Biomed Mater Res Part A: 105A: 547-556, 2017.
27750379	12	4	dep	Res	1500:1502	arg1	A					1509:1509	Part A	1504:1509	J Biomed Mater Res Part A: 105A: 547-556, 2017.	1485:1531	J Biomed Mater Res Part A: 105A: 547-556, 2017.
27750379	1	5	with	biomaterials	150:161	arg1	properties					174:183	novel properties	168:183	novel properties for biomedical purposes	168:207	Today, there is a need for the development of biomaterials with novel properties for biomedical purposes.
27750379	6	6	theme	mechanical	796:805	arg1	strength					807:814	their mechanical strength	790:814	their mechanical strength	790:814	The addition of chitosan to cryogels can increase their mechanical strength, cause change in surface morphology, increase the degradation time, and enhance adhesion to biological tissues.
27750379	1	7	theme	biomaterials	150:161	arg1	development					135:145	the development	131:145	the development of biomaterials with novel properties for biomedical purposes	131:207	Today, there is a need for the development of biomaterials with novel properties for biomedical purposes.
27750379	9	8	theme	moderate	1167:1174	arg1	cytotoxicity					1176:1187	moderate cytotoxicity	1167:1187	moderate cytotoxicity	1167:1187	Unlike cryogels based on HP, which exhibited moderate cytotoxicity, cryogels based on AP exhibited light cytotoxicity.
27750379	5	9	theme	pectin	707:712	arg1	features					655:662	structural features	644:662	structural features	644:662	The properties of pectin-chitosan cryogels were found to depend on the structural features and physicochemical characteristics of the pectin and chitosan within them.
27750379	5	9	theme	pectin	707:712	arg1	characteristics					684:698	physicochemical characteristics	668:698	physicochemical characteristics	668:698	The properties of pectin-chitosan cryogels were found to depend on the structural features and physicochemical characteristics of the pectin and chitosan within them.
27750379	10	10	theme	low	1322:1324	arg1	level					1326:1330	a low level	1320:1330	light cytotoxicity data as well as a low level of hemolysis of composite cryogels based on AP and chitosan	1285:1390	Based on the results of low immunogenicity, light cytotoxicity data as well as a low level of hemolysis of composite cryogels based on AP and chitosan are biocompatible and can potentially be used in biomedicine.
27750379	10	10	theme	low	1322:1324	arg1	biocompatible					1396:1408	biocompatible	1396:1408	biocompatible	1396:1408	Based on the results of low immunogenicity, light cytotoxicity data as well as a low level of hemolysis of composite cryogels based on AP and chitosan are biocompatible and can potentially be used in biomedicine.
27750379	10	11	theme	cryogels	1358:1365	arg1	hemolysis					1335:1343	hemolysis	1335:1343	hemolysis of composite cryogels based on AP and chitosan	1335:1390	Based on the results of low immunogenicity, light cytotoxicity data as well as a low level of hemolysis of composite cryogels based on AP and chitosan are biocompatible and can potentially be used in biomedicine.
27750379	6	12	theme	biological	908:917	arg1	tissues					919:925	biological tissues	908:925	biological tissues	908:925	The addition of chitosan to cryogels can increase their mechanical strength, cause change in surface morphology, increase the degradation time, and enhance adhesion to biological tissues.
27750379	2	13	theme	possible	279:286	arg1	use					288:290	its possible use	275:290	its possible use in biomedicine	275:305	The biocompatibility of materials is a key factor in determining its possible use in biomedicine.
27750379	8	14	theme	cells	1088:1092	arg1	hemolysis					1094:1102	red blood cells hemolysis	1078:1102	red blood cells hemolysis	1078:1102	Cryogels based on AP and HP were hemocompatible and the percentage of red blood cells hemolysis was less than 5%.
27750379	5	15	theme	chitosan	718:725	arg1	features					655:662	structural features	644:662	structural features	644:662	The properties of pectin-chitosan cryogels were found to depend on the structural features and physicochemical characteristics of the pectin and chitosan within them.
27750379	5	15	theme	chitosan	718:725	arg1	characteristics					684:698	physicochemical characteristics	668:698	physicochemical characteristics	668:698	The properties of pectin-chitosan cryogels were found to depend on the structural features and physicochemical characteristics of the pectin and chitosan within them.
27750379	6	16	theme	degradation	866:876	arg1	time					878:881	the degradation time	862:881	the degradation time	862:881	The addition of chitosan to cryogels can increase their mechanical strength, cause change in surface morphology, increase the degradation time, and enhance adhesion to biological tissues.
27750379	4	17	theme	Heracleum	462:470	arg1	pectin					475:480	Heracleum L. pectin	462:480	Heracleum L. pectin (HP)	462:485	For cryogel preparation, apple pectin (AP), Heracleum L. pectin (HP), and chitosan samples with different physical and chemical characteristics were used.
27750379	4	17	theme	Heracleum	462:470	arg1	HP					483:484	HP	483:484	HP	483:484	For cryogel preparation, apple pectin (AP), Heracleum L. pectin (HP), and chitosan samples with different physical and chemical characteristics were used.
27750379	0	18	theme	pectin	47:52	arg1	biocompatibility					16:31	biocompatibility	16:31	biocompatibility	16:31	Preparation and biocompatibility evaluation of pectin and chitosan cryogels for biomedical application.
27750379	0	18	theme	pectin	47:52	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and biocompatibility evaluation of pectin and chitosan cryogels for biomedical application.
27750379	10	19	theme	composite	1348:1356	arg1	cryogels					1358:1365	composite cryogels	1348:1365	composite cryogels based on AP and chitosan	1348:1390	Based on the results of low immunogenicity, light cytotoxicity data as well as a low level of hemolysis of composite cryogels based on AP and chitosan are biocompatible and can potentially be used in biomedicine.
27750379	4	20	with	samples	501:507	arg1	characteristics					546:560	different physical and chemical characteristics	514:560	different physical and chemical characteristics	514:560	For cryogel preparation, apple pectin (AP), Heracleum L. pectin (HP), and chitosan samples with different physical and chemical characteristics were used.
27750379	1	21	theme	novel	168:172	arg1	properties					174:183	novel properties	168:183	novel properties for biomedical purposes	168:207	Today, there is a need for the development of biomaterials with novel properties for biomedical purposes.
27750379	10	22	used	used	1433:1436	arg2	data					1304:1307	light cytotoxicity data	1285:1307	light cytotoxicity data as well as a low level of hemolysis of composite cryogels based on AP and chitosan	1285:1390	Based on the results of low immunogenicity, light cytotoxicity data as well as a low level of hemolysis of composite cryogels based on AP and chitosan are biocompatible and can potentially be used in biomedicine.
27750379	5	23	theme	cryogels	607:614	arg1	properties					577:586	The properties	573:586	The properties of pectin-chitosan cryogels	573:614	The properties of pectin-chitosan cryogels were found to depend on the structural features and physicochemical characteristics of the pectin and chitosan within them.
27750379	12	24	dep	105A	1512:1515	arg1	547-556					1518:1524	547-556	1518:1524	547-556	1518:1524	J Biomed Mater Res Part A: 105A: 547-556, 2017.
27750379	6	25	theme	chitosan	756:763	arg1	addition					744:751	The addition	740:751	The addition of chitosan to cryogels	740:775	The addition of chitosan to cryogels can increase their mechanical strength, cause change in surface morphology, increase the degradation time, and enhance adhesion to biological tissues.
27750379	6	26	from	change	823:828	arg1	morphology					841:850	surface morphology	833:850	surface morphology	833:850	The addition of chitosan to cryogels can increase their mechanical strength, cause change in surface morphology, increase the degradation time, and enhance adhesion to biological tissues.
27750379	10	27	theme	hemolysis	1335:1343	arg1	data					1304:1307	light cytotoxicity data	1285:1307	light cytotoxicity data as well as a low level of hemolysis of composite cryogels based on AP and chitosan	1285:1390	Based on the results of low immunogenicity, light cytotoxicity data as well as a low level of hemolysis of composite cryogels based on AP and chitosan are biocompatible and can potentially be used in biomedicine.
27750379	10	27	theme	hemolysis	1335:1343	arg1	level					1326:1330	a low level	1320:1330	light cytotoxicity data as well as a low level of hemolysis of composite cryogels based on AP and chitosan	1285:1390	Based on the results of low immunogenicity, light cytotoxicity data as well as a low level of hemolysis of composite cryogels based on AP and chitosan are biocompatible and can potentially be used in biomedicine.
27750379	10	27	theme	hemolysis	1335:1343	arg1	biocompatible					1396:1408	biocompatible	1396:1408	biocompatible	1396:1408	Based on the results of low immunogenicity, light cytotoxicity data as well as a low level of hemolysis of composite cryogels based on AP and chitosan are biocompatible and can potentially be used in biomedicine.
27750379	5	28	theme	physicochemical	668:682	arg1	characteristics					684:698	physicochemical characteristics	668:698	physicochemical characteristics	668:698	The properties of pectin-chitosan cryogels were found to depend on the structural features and physicochemical characteristics of the pectin and chitosan within them.
27750379	1	29	theme	biomedical	189:198	arg1	purposes					200:207	biomedical purposes	189:207	biomedical purposes	189:207	Today, there is a need for the development of biomaterials with novel properties for biomedical purposes.
27750379	4	30	theme	physical	524:531	arg1	characteristics					546:560	different physical and chemical characteristics	514:560	different physical and chemical characteristics	514:560	For cryogel preparation, apple pectin (AP), Heracleum L. pectin (HP), and chitosan samples with different physical and chemical characteristics were used.
27750379	4	31	theme	apple	443:447	arg1	AP					457:458	AP	457:458	AP	457:458	For cryogel preparation, apple pectin (AP), Heracleum L. pectin (HP), and chitosan samples with different physical and chemical characteristics were used.
27750379	4	31	theme	apple	443:447	arg1	pectin					449:454	apple pectin	443:454	apple pectin (AP)	443:459	For cryogel preparation, apple pectin (AP), Heracleum L. pectin (HP), and chitosan samples with different physical and chemical characteristics were used.
27750379	9	32	theme	light	1221:1225	arg1	cytotoxicity					1227:1238	light cytotoxicity	1221:1238	light cytotoxicity	1221:1238	Unlike cryogels based on HP, which exhibited moderate cytotoxicity, cryogels based on AP exhibited light cytotoxicity.
27750379	3	33	theme	cryotropic	397:406	arg1	gelation					408:415	ionic cryotropic gelation	391:415	ionic cryotropic gelation	391:415	In this study, composite cryogels were obtained based on pectin and chitosan using ionic cryotropic gelation.
27750379	4	34	theme	different	514:522	arg1	characteristics					546:560	different physical and chemical characteristics	514:560	different physical and chemical characteristics	514:560	For cryogel preparation, apple pectin (AP), Heracleum L. pectin (HP), and chitosan samples with different physical and chemical characteristics were used.
27750379	4	35	used	used	567:570	arg2	samples					501:507	apple pectin (AP), Heracleum L. pectin (HP), and chitosan samples	443:507	apple pectin (AP), Heracleum L. pectin (HP), and chitosan samples with different physical and chemical characteristics	443:560	For cryogel preparation, apple pectin (AP), Heracleum L. pectin (HP), and chitosan samples with different physical and chemical characteristics were used.
27750379	6	36	theme	surface	833:839	arg1	morphology					841:850	surface morphology	833:850	surface morphology	833:850	The addition of chitosan to cryogels can increase their mechanical strength, cause change in surface morphology, increase the degradation time, and enhance adhesion to biological tissues.
27750379	2	37	theme	materials	234:242	arg1	biocompatibility					214:229	The biocompatibility	210:229	The biocompatibility of materials	210:242	The biocompatibility of materials is a key factor in determining its possible use in biomedicine.
27750379	2	37	theme	materials	234:242	arg1	factor					253:258	a key factor	247:258	a key factor	247:258	The biocompatibility of materials is a key factor in determining its possible use in biomedicine.
27750379	10	38	theme	light	1285:1289	arg1	data					1304:1307	light cytotoxicity data	1285:1307	light cytotoxicity data as well as a low level of hemolysis of composite cryogels based on AP and chitosan	1285:1390	Based on the results of low immunogenicity, light cytotoxicity data as well as a low level of hemolysis of composite cryogels based on AP and chitosan are biocompatible and can potentially be used in biomedicine.
27750379	0	39	theme	chitosan	58:65	arg1	biocompatibility					16:31	biocompatibility	16:31	biocompatibility	16:31	Preparation and biocompatibility evaluation of pectin and chitosan cryogels for biomedical application.
27750379	0	39	theme	chitosan	58:65	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and biocompatibility evaluation of pectin and chitosan cryogels for biomedical application.
27750379	5	40	dep	features	655:662	arg1	the					640:642	the	640:642	the	640:642	The properties of pectin-chitosan cryogels were found to depend on the structural features and physicochemical characteristics of the pectin and chitosan within them.
27750379	4	41	theme	pectin	449:454	arg1	samples					501:507	apple pectin (AP), Heracleum L. pectin (HP), and chitosan samples	443:507	apple pectin (AP), Heracleum L. pectin (HP), and chitosan samples with different physical and chemical characteristics	443:560	For cryogel preparation, apple pectin (AP), Heracleum L. pectin (HP), and chitosan samples with different physical and chemical characteristics were used.
27750379	8	42	theme	blood	1082:1086	arg1	hemolysis					1094:1102	red blood cells hemolysis	1078:1102	red blood cells hemolysis	1078:1102	Cryogels based on AP and HP were hemocompatible and the percentage of red blood cells hemolysis was less than 5%.
27750379	3	43	theme	ionic	391:395	arg1	gelation					408:415	ionic cryotropic gelation	391:415	ionic cryotropic gelation	391:415	In this study, composite cryogels were obtained based on pectin and chitosan using ionic cryotropic gelation.
27750379	4	44	theme	chitosan	492:499	arg1	samples					501:507	apple pectin (AP), Heracleum L. pectin (HP), and chitosan samples	443:507	apple pectin (AP), Heracleum L. pectin (HP), and chitosan samples with different physical and chemical characteristics	443:560	For cryogel preparation, apple pectin (AP), Heracleum L. pectin (HP), and chitosan samples with different physical and chemical characteristics were used.
27750379	5	45	theme	structural	644:653	arg1	features					655:662	structural features	644:662	structural features	644:662	The properties of pectin-chitosan cryogels were found to depend on the structural features and physicochemical characteristics of the pectin and chitosan within them.
27750379	5	46	theme	pectin-chitosan	591:605	arg1	cryogels					607:614	pectin-chitosan cryogels	591:614	pectin-chitosan cryogels	591:614	The properties of pectin-chitosan cryogels were found to depend on the structural features and physicochemical characteristics of the pectin and chitosan within them.
27750379	10	47	theme	immunogenicity	1269:1282	arg1	results					1254:1260	the results	1250:1260	the results of low immunogenicity	1250:1282	Based on the results of low immunogenicity, light cytotoxicity data as well as a low level of hemolysis of composite cryogels based on AP and chitosan are biocompatible and can potentially be used in biomedicine.
27750379	11	48	dep	©	1454:1454	arg1	Inc.					1480:1483	Inc.	1480:1483	Inc.	1480:1483	© 2016 Wiley Periodicals, Inc.
27750379	12	49	theme	Part	1504:1507	arg1	A					1509:1509	Part A	1504:1509	J Biomed Mater Res Part A: 105A: 547-556, 2017.	1485:1531	J Biomed Mater Res Part A: 105A: 547-556, 2017.
27750379	5	50	dep	pectin	707:712	arg1	the					703:705	the	703:705	the	703:705	The properties of pectin-chitosan cryogels were found to depend on the structural features and physicochemical characteristics of the pectin and chitosan within them.
27750379	10	51	theme	low	1265:1267	arg1	immunogenicity					1269:1282	low immunogenicity	1265:1282	low immunogenicity	1265:1282	Based on the results of low immunogenicity, light cytotoxicity data as well as a low level of hemolysis of composite cryogels based on AP and chitosan are biocompatible and can potentially be used in biomedicine.
27750379	7	52	from	HP	1004:1005	arg1	cryogels					990:997	cryogels	990:997	cryogels from HP	990:1005	Cryogels based on AP were less immunogenic when compared with cryogels from HP.
27750379	8	53	theme	red	1078:1080	arg1	hemolysis					1094:1102	red blood cells hemolysis	1078:1102	red blood cells hemolysis	1078:1102	Cryogels based on AP and HP were hemocompatible and the percentage of red blood cells hemolysis was less than 5%.
27750379	2	54	from	use	288:290	arg1	biomedicine					295:305	biomedicine	295:305	biomedicine	295:305	The biocompatibility of materials is a key factor in determining its possible use in biomedicine.
27750379	8	55	theme	hemolysis	1094:1102	arg1	%					1119:1119	less than 5%	1108:1119	less than 5%	1108:1119	Cryogels based on AP and HP were hemocompatible and the percentage of red blood cells hemolysis was less than 5%.
27750379	8	55	theme	hemolysis	1094:1102	arg1	percentage					1064:1073	the percentage	1060:1073	the percentage of red blood cells hemolysis	1060:1102	Cryogels based on AP and HP were hemocompatible and the percentage of red blood cells hemolysis was less than 5%.
27750379	4	56	theme	cryogel	422:428	arg1	preparation					430:440	cryogel preparation	422:440	cryogel preparation	422:440	For cryogel preparation, apple pectin (AP), Heracleum L. pectin (HP), and chitosan samples with different physical and chemical characteristics were used.
27750379	2	57	theme	key	249:251	arg1	biocompatibility					214:229	The biocompatibility	210:229	The biocompatibility of materials	210:242	The biocompatibility of materials is a key factor in determining its possible use in biomedicine.
27750379	2	57	theme	key	249:251	arg1	factor					253:258	a key factor	247:258	a key factor	247:258	The biocompatibility of materials is a key factor in determining its possible use in biomedicine.
27750379	3	58	theme	composite	323:331	arg1	cryogels					333:340	composite cryogels	323:340	composite cryogels	323:340	In this study, composite cryogels were obtained based on pectin and chitosan using ionic cryotropic gelation.
27750379	4	59	theme	pectin	475:480	arg1	samples					501:507	apple pectin (AP), Heracleum L. pectin (HP), and chitosan samples	443:507	apple pectin (AP), Heracleum L. pectin (HP), and chitosan samples with different physical and chemical characteristics	443:560	For cryogel preparation, apple pectin (AP), Heracleum L. pectin (HP), and chitosan samples with different physical and chemical characteristics were used.
27750379	0	60	dep	Preparation	0:10	arg1	evaluation					33:42	evaluation	33:42	evaluation	33:42	Preparation and biocompatibility evaluation of pectin and chitosan cryogels for biomedical application.
27181577	4	0	theme	various	507:513	arg1	methods					515:521	various methods	507:521	various methods including Fourier transform infrared spectroscopy (FT-IR) and Surface morphology (SEM)	507:608	The hydrogels have been characterized by various methods including Fourier transform infrared spectroscopy (FT-IR) and Surface morphology (SEM).
27181577	8	1	theme	compressive	1054:1064	arg1	strength					1066:1073	the compressive strength	1050:1073	the compressive strength	1050:1073	Especially, the compressive strength and modulus increase 144% and 296% respectively as the GO content increase from 0 to 5mg/ml.
27181577	3	2	dep	Meanwhile	387:395	arg1	different					398:406	different	398:406	different	398:406	Meanwhile, different amount of graphene oxide (GO) was added as nano-additive.
27181577	11	3	theme	similar	1385:1391	arg1	modulus					1405:1411	the similar compressive modulus	1381:1411	the similar compressive modulus with soft tissue and excellent biocompatibility	1381:1459	Therefore, due to the appropriate water absorption capacity, the similar compressive modulus with soft tissue and excellent biocompatibility, the composite hydrogels have potential application in wound dressings.
27181577	11	3	theme	similar	1385:1391	arg1	capacity					1371:1378	the appropriate water absorption capacity	1338:1378	the appropriate water absorption capacity	1338:1378	Therefore, due to the appropriate water absorption capacity, the similar compressive modulus with soft tissue and excellent biocompatibility, the composite hydrogels have potential application in wound dressings.
27181577	11	4	theme	water	1354:1358	arg1	modulus					1405:1411	the similar compressive modulus	1381:1411	the similar compressive modulus with soft tissue and excellent biocompatibility	1381:1459	Therefore, due to the appropriate water absorption capacity, the similar compressive modulus with soft tissue and excellent biocompatibility, the composite hydrogels have potential application in wound dressings.
27181577	11	4	theme	water	1354:1358	arg1	capacity					1371:1378	the appropriate water absorption capacity	1338:1378	the appropriate water absorption capacity	1338:1378	Therefore, due to the appropriate water absorption capacity, the similar compressive modulus with soft tissue and excellent biocompatibility, the composite hydrogels have potential application in wound dressings.
27181577	6	5	theme	swelling	830:837	arg1	ability					839:845	swelling ability	830:845	swelling ability	830:845	In addition, the influence of different GO content on properties including gelation time, swelling ability, water evaporation rate and mechanical properties was investigated.
27181577	10	6	with	hydrogels	1272:1280	arg1	GO					1287:1288	GO	1287:1288	GO	1287:1288	The result indicate that hydrogels with GO show better biocompatibility.
27181577	4	7	dep	transform	541:549	arg1	infrared					551:558	infrared	551:558	transform infrared spectroscopy (FT-IR) and Surface morphology (SEM)	541:608	The hydrogels have been characterized by various methods including Fourier transform infrared spectroscopy (FT-IR) and Surface morphology (SEM).
27181577	7	8	theme	swelling	993:1000	arg1	ability					1002:1008	appropriate swelling ability	981:1008	appropriate swelling ability	981:1008	The results indicate that the hydrogels have short gelation time, appropriate swelling ability and water evaporation rate.
27181577	3	9	theme	Meanwhile	387:395	arg1	amount					408:413	Meanwhile, different amount	387:413	Meanwhile, different amount of graphene oxide (GO)	387:436	Meanwhile, different amount of graphene oxide (GO) was added as nano-additive.
27181577	3	9	theme	Meanwhile	387:395	arg1	oxide					427:431	graphene oxide	418:431	graphene oxide (GO)	418:436	Meanwhile, different amount of graphene oxide (GO) was added as nano-additive.
27181577	3	9	theme	Meanwhile	387:395	arg1	nano-additive					451:463	nano-additive	451:463	nano-additive	451:463	Meanwhile, different amount of graphene oxide (GO) was added as nano-additive.
27181577	11	10	theme	compressive	1393:1403	arg1	modulus					1405:1411	the similar compressive modulus	1381:1411	the similar compressive modulus with soft tissue and excellent biocompatibility	1381:1459	Therefore, due to the appropriate water absorption capacity, the similar compressive modulus with soft tissue and excellent biocompatibility, the composite hydrogels have potential application in wound dressings.
27181577	11	10	theme	compressive	1393:1403	arg1	capacity					1371:1378	the appropriate water absorption capacity	1338:1378	the appropriate water absorption capacity	1338:1378	Therefore, due to the appropriate water absorption capacity, the similar compressive modulus with soft tissue and excellent biocompatibility, the composite hydrogels have potential application in wound dressings.
27181577	11	11	theme	absorption	1360:1369	arg1	modulus					1405:1411	the similar compressive modulus	1381:1411	the similar compressive modulus with soft tissue and excellent biocompatibility	1381:1459	Therefore, due to the appropriate water absorption capacity, the similar compressive modulus with soft tissue and excellent biocompatibility, the composite hydrogels have potential application in wound dressings.
27181577	11	11	theme	absorption	1360:1369	arg1	capacity					1371:1378	the appropriate water absorption capacity	1338:1378	the appropriate water absorption capacity	1338:1378	Therefore, due to the appropriate water absorption capacity, the similar compressive modulus with soft tissue and excellent biocompatibility, the composite hydrogels have potential application in wound dressings.
27181577	6	12	theme	gelation	815:822	arg1	time					824:827	gelation time	815:827	gelation time	815:827	In addition, the influence of different GO content on properties including gelation time, swelling ability, water evaporation rate and mechanical properties was investigated.
27181577	2	13	theme	glucomannan	320:330	arg1	aldehyde					292:299	the aldehyde	288:299	the aldehyde of oxidized konjac glucomannan (OKGM) and the amino of carboxymethyl chitosan (CMCS)	288:384	In this article, composite hydrogels were prepared through the Schiff-base reaction between the aldehyde of oxidized konjac glucomannan (OKGM) and the amino of carboxymethyl chitosan (CMCS).
27181577	9	14	theme	MTT	1178:1180	arg1	assay					1182:1186	MTT assay	1178:1186	MTT assay	1178:1186	Moreover, MTT assay was applied to evaluate the biocompatibility of hydrogels.
27181577	3	15	theme	oxide	427:431	arg1	amount					408:413	Meanwhile, different amount	387:413	Meanwhile, different amount of graphene oxide (GO)	387:436	Meanwhile, different amount of graphene oxide (GO) was added as nano-additive.
27181577	3	15	theme	oxide	427:431	arg1	oxide					427:431	graphene oxide	418:431	graphene oxide (GO)	418:436	Meanwhile, different amount of graphene oxide (GO) was added as nano-additive.
27181577	3	15	theme	oxide	427:431	arg1	nano-additive					451:463	nano-additive	451:463	nano-additive	451:463	Meanwhile, different amount of graphene oxide (GO) was added as nano-additive.
27181577	3	16	dep	oxide	427:431	arg1	GO					434:435	GO	434:435	GO	434:435	Meanwhile, different amount of graphene oxide (GO) was added as nano-additive.
27181577	1	17	from	biomaterials	162:173	arg1	field					189:193	biomedical field	178:193	biomedical field	178:193	Polysaccharide hydrogels have been widely used as biomaterials in biomedical field.
27181577	1	18	theme	Polysaccharide	112:125	arg1	biomaterials					162:173	biomaterials	162:173	biomaterials in biomedical field	162:193	Polysaccharide hydrogels have been widely used as biomaterials in biomedical field.
27181577	1	18	theme	Polysaccharide	112:125	arg1	hydrogels					127:135	Polysaccharide hydrogels	112:135	Polysaccharide hydrogels	112:135	Polysaccharide hydrogels have been widely used as biomaterials in biomedical field.
27181577	11	19	theme	excellent	1434:1442	arg1	biocompatibility					1444:1459	excellent biocompatibility	1434:1459	excellent biocompatibility	1434:1459	Therefore, due to the appropriate water absorption capacity, the similar compressive modulus with soft tissue and excellent biocompatibility, the composite hydrogels have potential application in wound dressings.
27181577	7	20	contain	have	955:958	arg2	ability					1002:1008	appropriate swelling ability	981:1008	appropriate swelling ability	981:1008	The results indicate that the hydrogels have short gelation time, appropriate swelling ability and water evaporation rate.
27181577	7	20	contain	have	955:958	arg1	hydrogels					945:953	the hydrogels	941:953	the hydrogels	941:953	The results indicate that the hydrogels have short gelation time, appropriate swelling ability and water evaporation rate.
27181577	7	20	contain	have	955:958	arg2	rate					1032:1035	water evaporation rate	1014:1035	water evaporation rate	1014:1035	The results indicate that the hydrogels have short gelation time, appropriate swelling ability and water evaporation rate.
27181577	7	20	contain	have	955:958	arg2	time					975:978	short gelation time	960:978	short gelation time	960:978	The results indicate that the hydrogels have short gelation time, appropriate swelling ability and water evaporation rate.
27181577	7	21	theme	gelation	966:973	arg1	time					975:978	short gelation time	960:978	short gelation time	960:978	The results indicate that the hydrogels have short gelation time, appropriate swelling ability and water evaporation rate.
27181577	2	22	theme	amino	347:351	arg1	aldehyde					292:299	the aldehyde	288:299	the aldehyde of oxidized konjac glucomannan (OKGM) and the amino of carboxymethyl chitosan (CMCS)	288:384	In this article, composite hydrogels were prepared through the Schiff-base reaction between the aldehyde of oxidized konjac glucomannan (OKGM) and the amino of carboxymethyl chitosan (CMCS).
27181577	7	23	theme	short	960:964	arg1	time					975:978	short gelation time	960:978	short gelation time	960:978	The results indicate that the hydrogels have short gelation time, appropriate swelling ability and water evaporation rate.
27181577	11	24	theme	appropriate	1342:1352	arg1	modulus					1405:1411	the similar compressive modulus	1381:1411	the similar compressive modulus with soft tissue and excellent biocompatibility	1381:1459	Therefore, due to the appropriate water absorption capacity, the similar compressive modulus with soft tissue and excellent biocompatibility, the composite hydrogels have potential application in wound dressings.
27181577	11	24	theme	appropriate	1342:1352	arg1	capacity					1371:1378	the appropriate water absorption capacity	1338:1378	the appropriate water absorption capacity	1338:1378	Therefore, due to the appropriate water absorption capacity, the similar compressive modulus with soft tissue and excellent biocompatibility, the composite hydrogels have potential application in wound dressings.
27181577	5	25	theme	pore	705:708	arg1	structure					710:718	a homogeneous interconnected pore structure	676:718	a homogeneous interconnected pore structure	676:718	Through the observation of SEM, the hydrogels' scaffolds present a homogeneous interconnected pore structure after lyophilizing.
27181577	9	26	theme	hydrogels	1236:1244	arg1	biocompatibility					1216:1231	the biocompatibility	1212:1231	the biocompatibility of hydrogels	1212:1244	Moreover, MTT assay was applied to evaluate the biocompatibility of hydrogels.
27181577	6	27	theme	content	783:789	arg1	influence					757:765	the influence	753:765	the influence of different GO content on properties including gelation time, swelling ability, water evaporation rate and mechanical properties	753:895	In addition, the influence of different GO content on properties including gelation time, swelling ability, water evaporation rate and mechanical properties was investigated.
27181577	7	28	theme	appropriate	981:991	arg1	ability					1002:1008	appropriate swelling ability	981:1008	appropriate swelling ability	981:1008	The results indicate that the hydrogels have short gelation time, appropriate swelling ability and water evaporation rate.
27181577	4	29	theme	Surface	585:591	arg1	SEM					605:607	SEM	605:607	SEM	605:607	The hydrogels have been characterized by various methods including Fourier transform infrared spectroscopy (FT-IR) and Surface morphology (SEM).
27181577	4	29	theme	Surface	585:591	arg1	morphology					593:602	Surface morphology	585:602	Surface morphology (SEM)	585:608	The hydrogels have been characterized by various methods including Fourier transform infrared spectroscopy (FT-IR) and Surface morphology (SEM).
27181577	2	30	theme	composite	213:221	arg1	hydrogels					223:231	composite hydrogels	213:231	composite hydrogels	213:231	In this article, composite hydrogels were prepared through the Schiff-base reaction between the aldehyde of oxidized konjac glucomannan (OKGM) and the amino of carboxymethyl chitosan (CMCS).
27181577	1	31	used	used	154:157	arg2	hydrogels					127:135	Polysaccharide hydrogels	112:135	Polysaccharide hydrogels	112:135	Polysaccharide hydrogels have been widely used as biomaterials in biomedical field.
27181577	1	31	used	used	154:157	arg2	biomaterials					162:173	biomaterials	162:173	biomaterials in biomedical field	162:193	Polysaccharide hydrogels have been widely used as biomaterials in biomedical field.
27181577	11	32	theme	composite	1466:1474	arg1	hydrogels					1476:1484	the composite hydrogels	1462:1484	the composite hydrogels	1462:1484	Therefore, due to the appropriate water absorption capacity, the similar compressive modulus with soft tissue and excellent biocompatibility, the composite hydrogels have potential application in wound dressings.
27181577	0	33	theme	konjac	45:50	arg1	glucomannan/carboxymethyl					52:76	oxidized konjac glucomannan/carboxymethyl	36:76	oxidized konjac glucomannan/carboxymethyl	36:76	Preparation and characterization of oxidized konjac glucomannan/carboxymethyl chitosan/graphene oxide hydrogel.
27181577	6	34	from	influence	757:765	arg1	properties					794:803	properties	794:803	properties including gelation time, swelling ability, water evaporation rate and mechanical properties	794:895	In addition, the influence of different GO content on properties including gelation time, swelling ability, water evaporation rate and mechanical properties was investigated.
27181577	6	34	from	influence	757:765	arg1	time					824:827	gelation time	815:827	gelation time	815:827	In addition, the influence of different GO content on properties including gelation time, swelling ability, water evaporation rate and mechanical properties was investigated.
27181577	6	34	from	influence	757:765	arg1	ability					839:845	swelling ability	830:845	swelling ability	830:845	In addition, the influence of different GO content on properties including gelation time, swelling ability, water evaporation rate and mechanical properties was investigated.
27181577	6	34	from	influence	757:765	arg1	rate					866:869	water evaporation rate	848:869	water evaporation rate	848:869	In addition, the influence of different GO content on properties including gelation time, swelling ability, water evaporation rate and mechanical properties was investigated.
27181577	6	34	from	influence	757:765	arg1	properties					886:895	mechanical properties	875:895	mechanical properties	875:895	In addition, the influence of different GO content on properties including gelation time, swelling ability, water evaporation rate and mechanical properties was investigated.
27181577	6	35	theme	mechanical	875:884	arg1	properties					886:895	mechanical properties	875:895	mechanical properties	875:895	In addition, the influence of different GO content on properties including gelation time, swelling ability, water evaporation rate and mechanical properties was investigated.
27181577	0	36	theme	oxidized	36:43	arg1	glucomannan/carboxymethyl					52:76	oxidized konjac glucomannan/carboxymethyl	36:76	oxidized konjac glucomannan/carboxymethyl	36:76	Preparation and characterization of oxidized konjac glucomannan/carboxymethyl chitosan/graphene oxide hydrogel.
27181577	4	37	dep	Fourier	533:539	arg1	transform					541:549	transform	541:549	transform infrared spectroscopy (FT-IR) and Surface morphology (SEM)	541:608	The hydrogels have been characterized by various methods including Fourier transform infrared spectroscopy (FT-IR) and Surface morphology (SEM).
27181577	6	38	theme	GO	780:781	arg1	content					783:789	different GO content	770:789	different GO content	770:789	In addition, the influence of different GO content on properties including gelation time, swelling ability, water evaporation rate and mechanical properties was investigated.
27181577	11	39	theme	soft	1418:1421	arg1	tissue					1423:1428	soft tissue	1418:1428	soft tissue	1418:1428	Therefore, due to the appropriate water absorption capacity, the similar compressive modulus with soft tissue and excellent biocompatibility, the composite hydrogels have potential application in wound dressings.
27181577	3	40	theme	graphene	418:425	arg1	oxide					427:431	graphene oxide	418:431	graphene oxide (GO)	418:436	Meanwhile, different amount of graphene oxide (GO) was added as nano-additive.
27181577	2	41	theme	Schiff-base	259:269	arg1	reaction					271:278	the Schiff-base reaction	255:278	the Schiff-base reaction between the aldehyde of oxidized konjac glucomannan (OKGM) and the amino of carboxymethyl chitosan (CMCS)	255:384	In this article, composite hydrogels were prepared through the Schiff-base reaction between the aldehyde of oxidized konjac glucomannan (OKGM) and the amino of carboxymethyl chitosan (CMCS).
27181577	6	42	theme	different	770:778	arg1	content					783:789	different GO content	770:789	different GO content	770:789	In addition, the influence of different GO content on properties including gelation time, swelling ability, water evaporation rate and mechanical properties was investigated.
27181577	0	43	theme	glucomannan/carboxymethyl	52:76	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of oxidized konjac glucomannan/carboxymethyl chitosan/graphene oxide hydrogel.
27181577	0	43	theme	glucomannan/carboxymethyl	52:76	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of oxidized konjac glucomannan/carboxymethyl chitosan/graphene oxide hydrogel.
27181577	5	44	theme	homogeneous	678:688	arg1	structure					710:718	a homogeneous interconnected pore structure	676:718	a homogeneous interconnected pore structure	676:718	Through the observation of SEM, the hydrogels' scaffolds present a homogeneous interconnected pore structure after lyophilizing.
27181577	6	45	theme	evaporation	854:864	arg1	rate					866:869	water evaporation rate	848:869	water evaporation rate	848:869	In addition, the influence of different GO content on properties including gelation time, swelling ability, water evaporation rate and mechanical properties was investigated.
27181577	5	46	theme	SEM	638:640	arg1	observation					623:633	the observation	619:633	the observation of SEM	619:640	Through the observation of SEM, the hydrogels' scaffolds present a homogeneous interconnected pore structure after lyophilizing.
27181577	11	47	from	application	1501:1511	arg1	dressings					1522:1530	wound dressings	1516:1530	wound dressings	1516:1530	Therefore, due to the appropriate water absorption capacity, the similar compressive modulus with soft tissue and excellent biocompatibility, the composite hydrogels have potential application in wound dressings.
27181577	2	48	theme	chitosan	370:377	arg1	OKGM					333:336	OKGM	333:336	OKGM	333:336	In this article, composite hydrogels were prepared through the Schiff-base reaction between the aldehyde of oxidized konjac glucomannan (OKGM) and the amino of carboxymethyl chitosan (CMCS).
27181577	2	48	theme	chitosan	370:377	arg1	glucomannan					320:330	oxidized konjac glucomannan	304:330	oxidized konjac glucomannan (OKGM)	304:337	In this article, composite hydrogels were prepared through the Schiff-base reaction between the aldehyde of oxidized konjac glucomannan (OKGM) and the amino of carboxymethyl chitosan (CMCS).
27181577	2	48	theme	chitosan	370:377	arg1	amino					347:351	the amino	343:351	the amino of carboxymethyl chitosan (CMCS)	343:384	In this article, composite hydrogels were prepared through the Schiff-base reaction between the aldehyde of oxidized konjac glucomannan (OKGM) and the amino of carboxymethyl chitosan (CMCS).
27181577	11	49	theme	wound	1516:1520	arg1	dressings					1522:1530	wound dressings	1516:1530	wound dressings	1516:1530	Therefore, due to the appropriate water absorption capacity, the similar compressive modulus with soft tissue and excellent biocompatibility, the composite hydrogels have potential application in wound dressings.
27181577	5	50	theme	interconnected	690:703	arg1	structure					710:718	a homogeneous interconnected pore structure	676:718	a homogeneous interconnected pore structure	676:718	Through the observation of SEM, the hydrogels' scaffolds present a homogeneous interconnected pore structure after lyophilizing.
27181577	6	51	theme	water	848:852	arg1	rate					866:869	water evaporation rate	848:869	water evaporation rate	848:869	In addition, the influence of different GO content on properties including gelation time, swelling ability, water evaporation rate and mechanical properties was investigated.
27181577	2	52	theme	oxidized	304:311	arg1	OKGM					333:336	OKGM	333:336	OKGM	333:336	In this article, composite hydrogels were prepared through the Schiff-base reaction between the aldehyde of oxidized konjac glucomannan (OKGM) and the amino of carboxymethyl chitosan (CMCS).
27181577	2	52	theme	oxidized	304:311	arg1	glucomannan					320:330	oxidized konjac glucomannan	304:330	oxidized konjac glucomannan (OKGM)	304:337	In this article, composite hydrogels were prepared through the Schiff-base reaction between the aldehyde of oxidized konjac glucomannan (OKGM) and the amino of carboxymethyl chitosan (CMCS).
27181577	8	53	theme	GO	1130:1131	arg1	content					1133:1139	the GO content	1126:1139	the GO content	1126:1139	Especially, the compressive strength and modulus increase 144% and 296% respectively as the GO content increase from 0 to 5mg/ml.
27181577	2	54	theme	carboxymethyl	356:368	arg1	chitosan					370:377	carboxymethyl chitosan	356:377	carboxymethyl chitosan (CMCS)	356:384	In this article, composite hydrogels were prepared through the Schiff-base reaction between the aldehyde of oxidized konjac glucomannan (OKGM) and the amino of carboxymethyl chitosan (CMCS).
27181577	2	54	theme	carboxymethyl	356:368	arg1	CMCS					380:383	CMCS	380:383	CMCS	380:383	In this article, composite hydrogels were prepared through the Schiff-base reaction between the aldehyde of oxidized konjac glucomannan (OKGM) and the amino of carboxymethyl chitosan (CMCS).
27181577	2	55	theme	konjac	313:318	arg1	OKGM					333:336	OKGM	333:336	OKGM	333:336	In this article, composite hydrogels were prepared through the Schiff-base reaction between the aldehyde of oxidized konjac glucomannan (OKGM) and the amino of carboxymethyl chitosan (CMCS).
27181577	2	55	theme	konjac	313:318	arg1	glucomannan					320:330	oxidized konjac glucomannan	304:330	oxidized konjac glucomannan (OKGM)	304:337	In this article, composite hydrogels were prepared through the Schiff-base reaction between the aldehyde of oxidized konjac glucomannan (OKGM) and the amino of carboxymethyl chitosan (CMCS).
27181577	7	56	theme	water	1014:1018	arg1	rate					1032:1035	water evaporation rate	1014:1035	water evaporation rate	1014:1035	The results indicate that the hydrogels have short gelation time, appropriate swelling ability and water evaporation rate.
27181577	11	57	theme	potential	1491:1499	arg1	application					1501:1511	potential application	1491:1511	potential application in wound dressings	1491:1530	Therefore, due to the appropriate water absorption capacity, the similar compressive modulus with soft tissue and excellent biocompatibility, the composite hydrogels have potential application in wound dressings.
27181577	8	58	dep	0	1155:1155	arg1	to					1157:1158	to	1157:1158	to	1157:1158	Especially, the compressive strength and modulus increase 144% and 296% respectively as the GO content increase from 0 to 5mg/ml.
27181577	8	58	dep	0	1155:1155	arg1	5mg/ml					1160:1165	5mg/ml	1160:1165	0 to 5mg/ml	1155:1165	Especially, the compressive strength and modulus increase 144% and 296% respectively as the GO content increase from 0 to 5mg/ml.
27181577	7	59	theme	evaporation	1020:1030	arg1	rate					1032:1035	water evaporation rate	1014:1035	water evaporation rate	1014:1035	The results indicate that the hydrogels have short gelation time, appropriate swelling ability and water evaporation rate.
27181577	1	60	theme	biomedical	178:187	arg1	field					189:193	biomedical field	178:193	biomedical field	178:193	Polysaccharide hydrogels have been widely used as biomaterials in biomedical field.
27181577	11	61	contain	have	1486:1489	arg1	hydrogels					1476:1484	the composite hydrogels	1462:1484	the composite hydrogels	1462:1484	Therefore, due to the appropriate water absorption capacity, the similar compressive modulus with soft tissue and excellent biocompatibility, the composite hydrogels have potential application in wound dressings.
27181577	11	61	contain	have	1486:1489	arg2	application					1501:1511	potential application	1491:1511	potential application in wound dressings	1491:1530	Therefore, due to the appropriate water absorption capacity, the similar compressive modulus with soft tissue and excellent biocompatibility, the composite hydrogels have potential application in wound dressings.
27181577	0	62	theme	oxide	96:100	arg1	hydrogel					102:109	oxide hydrogel	96:109	oxide hydrogel	96:109	Preparation and characterization of oxidized konjac glucomannan/carboxymethyl chitosan/graphene oxide hydrogel.
27181577	11	63	with	modulus	1405:1411	arg1	tissue					1423:1428	soft tissue	1418:1428	soft tissue	1418:1428	Therefore, due to the appropriate water absorption capacity, the similar compressive modulus with soft tissue and excellent biocompatibility, the composite hydrogels have potential application in wound dressings.
27181577	11	63	with	modulus	1405:1411	arg1	biocompatibility					1444:1459	excellent biocompatibility	1434:1459	excellent biocompatibility	1434:1459	Therefore, due to the appropriate water absorption capacity, the similar compressive modulus with soft tissue and excellent biocompatibility, the composite hydrogels have potential application in wound dressings.
24472504	7	0	theme	scanning	1036:1043	arg1	calorimetry					1045:1055	Differential scanning calorimetry	1023:1055	Differential scanning calorimetry (DSC) analysis	1023:1070	Differential scanning calorimetry (DSC) analysis revealed unimodal melting peak indicating the α-crystalline form of PLA.
24472504	7	0	theme	scanning	1036:1043	arg1	DSC					1058:1060	DSC	1058:1060	DSC	1058:1060	Differential scanning calorimetry (DSC) analysis revealed unimodal melting peak indicating the α-crystalline form of PLA.
24472504	3	1	dep	wt	605:606	arg1	to					600:601	to	600:601	to	600:601	The differential TG analysis revealed that there is no change in the Tmax value (357 °C) for PLA and its composites up to 5 wt% of bio-filler loading.
24472504	5	2	theme	%	774:774	arg1	case					761:764	the case	757:764	the case of 10 wt% of the filler incorporated in the PLA matrix	757:819	In the case of 10 wt% of the filler incorporated in the PLA matrix, Tmax rapidly shifted to lower temperature (324 °C).
24472504	1	3	theme	thermal	206:212	arg1	behavior					226:233	the thermal degradation behavior	202:233	the thermal degradation behavior of poly(lactic acid) (PLA) biocomposites in order to render its suitability for food packaging application	202:340	The current work is focused on investigating the influence of novel bio-filler, "sucrose palmitate (SP)" on the thermal degradation behavior of poly(lactic acid) (PLA) biocomposites in order to render its suitability for food packaging application.
24472504	7	4	theme	calorimetry	1045:1055	arg1	analysis					1063:1070	Differential scanning calorimetry (DSC) analysis	1023:1070	Differential scanning calorimetry (DSC) analysis	1023:1070	Differential scanning calorimetry (DSC) analysis revealed unimodal melting peak indicating the α-crystalline form of PLA.
24472504	1	5	from	influence	143:151	arg1	behavior					226:233	the thermal degradation behavior	202:233	the thermal degradation behavior of poly(lactic acid) (PLA) biocomposites in order to render its suitability for food packaging application	202:340	The current work is focused on investigating the influence of novel bio-filler, "sucrose palmitate (SP)" on the thermal degradation behavior of poly(lactic acid) (PLA) biocomposites in order to render its suitability for food packaging application.
24472504	6	6	theme	filler	921:926	arg1	loading					906:912	higher loading	899:912	higher loading of the filler	899:926	This downturn in Tmax at higher loading of the filler is due to the increase in acidic sites and enhancement in the rate of degradation is observed.
24472504	9	7	dep	Flynn-Wall-Ozawa	1370:1385	arg1	methods					1401:1407	methods	1401:1407	methods	1401:1407	The activation energies (Ea) of thermal degradation of PLA and PLA composites were evaluated by Flynn-Wall-Ozawa and Kissinger methods.
24472504	1	8	theme	degradation	214:224	arg1	behavior					226:233	the thermal degradation behavior	202:233	the thermal degradation behavior of poly(lactic acid) (PLA) biocomposites in order to render its suitability for food packaging application	202:340	The current work is focused on investigating the influence of novel bio-filler, "sucrose palmitate (SP)" on the thermal degradation behavior of poly(lactic acid) (PLA) biocomposites in order to render its suitability for food packaging application.
24472504	3	9	theme	5	603:603	arg1	wt					605:606	wt	605:606	wt	605:606	The differential TG analysis revealed that there is no change in the Tmax value (357 °C) for PLA and its composites up to 5 wt% of bio-filler loading.
24472504	8	10	theme	composites	1250:1259	arg1	degradation					1231:1241	degradation	1231:1241	degradation of PLA composites	1231:1259	Based on the thermal degradation profile of sucrose palmitate, possible mechanism for degradation of PLA composites is proposed.
24472504	3	11	theme	TG	498:499	arg1	analysis					501:508	The differential TG analysis	481:508	The differential TG analysis	481:508	The differential TG analysis revealed that there is no change in the Tmax value (357 °C) for PLA and its composites up to 5 wt% of bio-filler loading.
24472504	7	12	theme	Differential	1023:1034	arg1	calorimetry					1045:1055	Differential scanning calorimetry	1023:1055	Differential scanning calorimetry (DSC) analysis	1023:1070	Differential scanning calorimetry (DSC) analysis revealed unimodal melting peak indicating the α-crystalline form of PLA.
24472504	7	12	theme	Differential	1023:1034	arg1	DSC					1058:1060	DSC	1058:1060	DSC	1058:1060	Differential scanning calorimetry (DSC) analysis revealed unimodal melting peak indicating the α-crystalline form of PLA.
24472504	3	13	theme	loading	623:629	arg1	%					607:607	up to 5 wt%	597:607	up to 5 wt% of bio-filler loading	597:629	The differential TG analysis revealed that there is no change in the Tmax value (357 °C) for PLA and its composites up to 5 wt% of bio-filler loading.
24472504	3	13	theme	loading	623:629	arg1	loading					623:629	bio-filler loading	612:629	bio-filler loading	612:629	The differential TG analysis revealed that there is no change in the Tmax value (357 °C) for PLA and its composites up to 5 wt% of bio-filler loading.
24472504	8	14	theme	thermal	1158:1164	arg1	profile					1178:1184	the thermal degradation profile	1154:1184	the thermal degradation profile of sucrose palmitate	1154:1205	Based on the thermal degradation profile of sucrose palmitate, possible mechanism for degradation of PLA composites is proposed.
24472504	4	15	theme	sucrose	654:660	arg1	barrier					693:699	a protective barrier	680:699	a protective barrier	680:699	This reveals that the sucrose palmitate acts as a protective barrier by decelerating the thermal degradation rate of PLA.
24472504	4	15	theme	sucrose	654:660	arg1	palmitate					662:670	the sucrose palmitate	650:670	the sucrose palmitate	650:670	This reveals that the sucrose palmitate acts as a protective barrier by decelerating the thermal degradation rate of PLA.
24472504	9	16	theme	PLA	1329:1331	arg1	degradation					1314:1324	thermal degradation	1306:1324	thermal degradation of PLA and PLA composites	1306:1350	The activation energies (Ea) of thermal degradation of PLA and PLA composites were evaluated by Flynn-Wall-Ozawa and Kissinger methods.
24472504	2	17	theme	heating	465:471	arg1	regime					473:478	dynamic heating regime	457:478	dynamic heating regime	457:478	Thermal degradation behavior of the PLA biocomposites was investigated by thermo-gravimetric analysis (TGA) using dynamic heating regime.
24472504	9	18	theme	thermal	1306:1312	arg1	degradation					1314:1324	thermal degradation	1306:1324	thermal degradation of PLA and PLA composites	1306:1350	The activation energies (Ea) of thermal degradation of PLA and PLA composites were evaluated by Flynn-Wall-Ozawa and Kissinger methods.
24472504	1	19	theme	novel	156:160	arg1	bio-filler					162:171	novel bio-filler	156:171	novel bio-filler	156:171	The current work is focused on investigating the influence of novel bio-filler, "sucrose palmitate (SP)" on the thermal degradation behavior of poly(lactic acid) (PLA) biocomposites in order to render its suitability for food packaging application.
24472504	2	20	theme	dynamic	457:463	arg1	regime					473:478	dynamic heating regime	457:478	dynamic heating regime	457:478	Thermal degradation behavior of the PLA biocomposites was investigated by thermo-gravimetric analysis (TGA) using dynamic heating regime.
24472504	0	21	theme	Thermal	0:6	arg1	kinetics					20:27	Thermal degradation kinetics	0:27	Thermal degradation kinetics of sucrose palmitate	0:48	Thermal degradation kinetics of sucrose palmitate reinforced poly(lactic acid) biocomposites.
24472504	1	22	theme	bio-filler	162:171	arg1	influence					143:151	the influence	139:151	the influence of novel bio-filler, "sucrose palmitate (SP)" on the thermal degradation behavior of poly(lactic acid) (PLA) biocomposites in order to render its suitability for food packaging application	139:340	The current work is focused on investigating the influence of novel bio-filler, "sucrose palmitate (SP)" on the thermal degradation behavior of poly(lactic acid) (PLA) biocomposites in order to render its suitability for food packaging application.
24472504	0	23	theme	degradation	8:18	arg1	kinetics					20:27	Thermal degradation kinetics	0:27	Thermal degradation kinetics of sucrose palmitate	0:48	Thermal degradation kinetics of sucrose palmitate reinforced poly(lactic acid) biocomposites.
24472504	3	24	theme	bio-filler	612:621	arg1	loading					623:629	bio-filler loading	612:629	bio-filler loading	612:629	The differential TG analysis revealed that there is no change in the Tmax value (357 °C) for PLA and its composites up to 5 wt% of bio-filler loading.
24472504	7	25	theme	α-crystalline	1118:1130	arg1	form					1132:1135	the α-crystalline form	1114:1135	the α-crystalline form of PLA	1114:1142	Differential scanning calorimetry (DSC) analysis revealed unimodal melting peak indicating the α-crystalline form of PLA.
24472504	6	26	from	downturn	879:886	arg1	Tmax					891:894	Tmax	891:894	Tmax	891:894	This downturn in Tmax at higher loading of the filler is due to the increase in acidic sites and enhancement in the rate of degradation is observed.
24472504	8	27	theme	palmitate	1197:1205	arg1	profile					1178:1184	the thermal degradation profile	1154:1184	the thermal degradation profile of sucrose palmitate	1154:1205	Based on the thermal degradation profile of sucrose palmitate, possible mechanism for degradation of PLA composites is proposed.
24472504	9	28	theme	activation	1278:1287	arg1	Ea					1299:1300	Ea	1299:1300	Ea	1299:1300	The activation energies (Ea) of thermal degradation of PLA and PLA composites were evaluated by Flynn-Wall-Ozawa and Kissinger methods.
24472504	9	28	theme	activation	1278:1287	arg1	energies					1289:1296	The activation energies	1274:1296	The activation energies (Ea) of thermal degradation of PLA and PLA composites	1274:1350	The activation energies (Ea) of thermal degradation of PLA and PLA composites were evaluated by Flynn-Wall-Ozawa and Kissinger methods.
24472504	2	29	theme	biocomposites	383:395	arg1	behavior					363:370	Thermal degradation behavior	343:370	Thermal degradation behavior of the PLA biocomposites	343:395	Thermal degradation behavior of the PLA biocomposites was investigated by thermo-gravimetric analysis (TGA) using dynamic heating regime.
24472504	1	30	theme	poly	238:241	arg1	biocomposites					262:274	poly(lactic acid) (PLA) biocomposites	238:274	poly(lactic acid) (PLA) biocomposites in order to render its suitability for food packaging application	238:340	The current work is focused on investigating the influence of novel bio-filler, "sucrose palmitate (SP)" on the thermal degradation behavior of poly(lactic acid) (PLA) biocomposites in order to render its suitability for food packaging application.
24472504	4	31	theme	PLA	749:751	arg1	rate					741:744	the thermal degradation rate	717:744	the thermal degradation rate of PLA	717:751	This reveals that the sucrose palmitate acts as a protective barrier by decelerating the thermal degradation rate of PLA.
24472504	5	32	theme	PLA	810:812	arg1	matrix					814:819	the PLA matrix	806:819	the PLA matrix	806:819	In the case of 10 wt% of the filler incorporated in the PLA matrix, Tmax rapidly shifted to lower temperature (324 °C).
24472504	8	33	theme	PLA	1246:1248	arg1	composites					1250:1259	PLA composites	1246:1259	PLA composites	1246:1259	Based on the thermal degradation profile of sucrose palmitate, possible mechanism for degradation of PLA composites is proposed.
24472504	6	34	theme	higher	899:904	arg1	loading					906:912	higher loading	899:912	higher loading of the filler	899:926	This downturn in Tmax at higher loading of the filler is due to the increase in acidic sites and enhancement in the rate of degradation is observed.
24472504	2	35	theme	PLA	379:381	arg1	biocomposites					383:395	the PLA biocomposites	375:395	the PLA biocomposites	375:395	Thermal degradation behavior of the PLA biocomposites was investigated by thermo-gravimetric analysis (TGA) using dynamic heating regime.
24472504	8	36	theme	possible	1208:1215	arg1	mechanism					1217:1225	possible mechanism	1208:1225	possible mechanism for degradation of PLA composites	1208:1259	Based on the thermal degradation profile of sucrose palmitate, possible mechanism for degradation of PLA composites is proposed.
24472504	3	37	from	change	536:541	arg1	value					555:559	the Tmax value	546:559	the Tmax value (357 °C) for PLA and its composites up to 5 wt% of bio-filler loading	546:629	The differential TG analysis revealed that there is no change in the Tmax value (357 °C) for PLA and its composites up to 5 wt% of bio-filler loading.
24472504	3	37	from	change	536:541	arg1	°C					566:567	357 °C	562:567	357 °C	562:567	The differential TG analysis revealed that there is no change in the Tmax value (357 °C) for PLA and its composites up to 5 wt% of bio-filler loading.
24472504	8	38	theme	sucrose	1189:1195	arg1	palmitate					1197:1205	sucrose palmitate	1189:1205	sucrose palmitate	1189:1205	Based on the thermal degradation profile of sucrose palmitate, possible mechanism for degradation of PLA composites is proposed.
24472504	1	39	theme	lactic	243:248	arg1	poly					238:241	poly	238:241	poly(lactic acid) (PLA) biocomposites in order to render its suitability for food packaging application	238:340	The current work is focused on investigating the influence of novel bio-filler, "sucrose palmitate (SP)" on the thermal degradation behavior of poly(lactic acid) (PLA) biocomposites in order to render its suitability for food packaging application.
24472504	1	39	theme	lactic	243:248	arg1	acid					250:253	lactic acid	243:253	lactic acid	243:253	The current work is focused on investigating the influence of novel bio-filler, "sucrose palmitate (SP)" on the thermal degradation behavior of poly(lactic acid) (PLA) biocomposites in order to render its suitability for food packaging application.
24472504	1	40	theme	food	315:318	arg1	application					330:340	food packaging application	315:340	food packaging application	315:340	The current work is focused on investigating the influence of novel bio-filler, "sucrose palmitate (SP)" on the thermal degradation behavior of poly(lactic acid) (PLA) biocomposites in order to render its suitability for food packaging application.
24472504	1	41	theme	current	98:104	arg1	work					106:109	The current work	94:109	The current work	94:109	The current work is focused on investigating the influence of novel bio-filler, "sucrose palmitate (SP)" on the thermal degradation behavior of poly(lactic acid) (PLA) biocomposites in order to render its suitability for food packaging application.
24472504	7	42	theme	PLA	1140:1142	arg1	form					1132:1135	the α-crystalline form	1114:1135	the α-crystalline form of PLA	1114:1142	Differential scanning calorimetry (DSC) analysis revealed unimodal melting peak indicating the α-crystalline form of PLA.
24472504	0	43	theme	palmitate	40:48	arg1	kinetics					20:27	Thermal degradation kinetics	0:27	Thermal degradation kinetics of sucrose palmitate	0:48	Thermal degradation kinetics of sucrose palmitate reinforced poly(lactic acid) biocomposites.
24472504	9	44	theme	degradation	1314:1324	arg1	Ea					1299:1300	Ea	1299:1300	Ea	1299:1300	The activation energies (Ea) of thermal degradation of PLA and PLA composites were evaluated by Flynn-Wall-Ozawa and Kissinger methods.
24472504	9	44	theme	degradation	1314:1324	arg1	energies					1289:1296	The activation energies	1274:1296	The activation energies (Ea) of thermal degradation of PLA and PLA composites	1274:1350	The activation energies (Ea) of thermal degradation of PLA and PLA composites were evaluated by Flynn-Wall-Ozawa and Kissinger methods.
24472504	1	45	theme	packaging	320:328	arg1	application					330:340	food packaging application	315:340	food packaging application	315:340	The current work is focused on investigating the influence of novel bio-filler, "sucrose palmitate (SP)" on the thermal degradation behavior of poly(lactic acid) (PLA) biocomposites in order to render its suitability for food packaging application.
24472504	6	46	from	enhancement	971:981	arg1	rate					990:993	the rate	986:993	the rate of degradation	986:1008	This downturn in Tmax at higher loading of the filler is due to the increase in acidic sites and enhancement in the rate of degradation is observed.
24472504	2	47	theme	degradation	351:361	arg1	behavior					363:370	Thermal degradation behavior	343:370	Thermal degradation behavior of the PLA biocomposites	343:395	Thermal degradation behavior of the PLA biocomposites was investigated by thermo-gravimetric analysis (TGA) using dynamic heating regime.
24472504	0	48	theme	sucrose	32:38	arg1	palmitate					40:48	sucrose palmitate	32:48	sucrose palmitate	32:48	Thermal degradation kinetics of sucrose palmitate reinforced poly(lactic acid) biocomposites.
24472504	2	49	theme	thermo-gravimetric	417:434	arg1	TGA					446:448	TGA	446:448	TGA	446:448	Thermal degradation behavior of the PLA biocomposites was investigated by thermo-gravimetric analysis (TGA) using dynamic heating regime.
24472504	2	49	theme	thermo-gravimetric	417:434	arg1	analysis					436:443	thermo-gravimetric analysis	417:443	thermo-gravimetric analysis (TGA) using dynamic heating regime	417:478	Thermal degradation behavior of the PLA biocomposites was investigated by thermo-gravimetric analysis (TGA) using dynamic heating regime.
24472504	6	50	from	increase	942:949	arg1	sites					961:965	acidic sites	954:965	acidic sites	954:965	This downturn in Tmax at higher loading of the filler is due to the increase in acidic sites and enhancement in the rate of degradation is observed.
24472504	2	51	theme	Thermal	343:349	arg1	behavior					363:370	Thermal degradation behavior	343:370	Thermal degradation behavior of the PLA biocomposites	343:395	Thermal degradation behavior of the PLA biocomposites was investigated by thermo-gravimetric analysis (TGA) using dynamic heating regime.
24472504	0	52	theme	poly	61:64	arg1	biocomposites					79:91	poly(lactic acid) biocomposites	61:91	poly(lactic acid) biocomposites	61:91	Thermal degradation kinetics of sucrose palmitate reinforced poly(lactic acid) biocomposites.
24472504	8	53	theme	degradation	1166:1176	arg1	profile					1178:1184	the thermal degradation profile	1154:1184	the thermal degradation profile of sucrose palmitate	1154:1205	Based on the thermal degradation profile of sucrose palmitate, possible mechanism for degradation of PLA composites is proposed.
24472504	4	54	theme	degradation	729:739	arg1	rate					741:744	the thermal degradation rate	717:744	the thermal degradation rate of PLA	717:751	This reveals that the sucrose palmitate acts as a protective barrier by decelerating the thermal degradation rate of PLA.
24472504	6	55	theme	acidic	954:959	arg1	sites					961:965	acidic sites	954:965	acidic sites	954:965	This downturn in Tmax at higher loading of the filler is due to the increase in acidic sites and enhancement in the rate of degradation is observed.
24472504	4	56	theme	thermal	721:727	arg1	rate					741:744	the thermal degradation rate	717:744	the thermal degradation rate of PLA	717:751	This reveals that the sucrose palmitate acts as a protective barrier by decelerating the thermal degradation rate of PLA.
24472504	9	57	dep	PLA	1329:1331	arg1	composites					1341:1350	composites	1341:1350	composites	1341:1350	The activation energies (Ea) of thermal degradation of PLA and PLA composites were evaluated by Flynn-Wall-Ozawa and Kissinger methods.
24472504	5	58	theme	filler	783:788	arg1	%					774:774	10 wt%	769:774	10 wt% of the filler incorporated in the PLA matrix	769:819	In the case of 10 wt% of the filler incorporated in the PLA matrix, Tmax rapidly shifted to lower temperature (324 °C).
24472504	5	58	theme	filler	783:788	arg1	filler					783:788	the filler	779:788	the filler incorporated in the PLA matrix	779:819	In the case of 10 wt% of the filler incorporated in the PLA matrix, Tmax rapidly shifted to lower temperature (324 °C).
24472504	6	59	from	loading	906:912	arg1	downturn					879:886	This downturn	874:886	This downturn in Tmax at higher loading of the filler	874:926	This downturn in Tmax at higher loading of the filler is due to the increase in acidic sites and enhancement in the rate of degradation is observed.
24472504	5	60	theme	lower	846:850	arg1	temperature					852:862	lower temperature	846:862	lower temperature (324 °C)	846:871	In the case of 10 wt% of the filler incorporated in the PLA matrix, Tmax rapidly shifted to lower temperature (324 °C).
24472504	5	60	theme	lower	846:850	arg1	°C					869:870	324 °C	865:870	324 °C	865:870	In the case of 10 wt% of the filler incorporated in the PLA matrix, Tmax rapidly shifted to lower temperature (324 °C).
24472504	4	61	theme	protective	682:691	arg1	barrier					693:699	a protective barrier	680:699	a protective barrier	680:699	This reveals that the sucrose palmitate acts as a protective barrier by decelerating the thermal degradation rate of PLA.
24472504	4	61	theme	protective	682:691	arg1	palmitate					662:670	the sucrose palmitate	650:670	the sucrose palmitate	650:670	This reveals that the sucrose palmitate acts as a protective barrier by decelerating the thermal degradation rate of PLA.
24472504	9	62	theme	PLA	1337:1339	arg1	degradation					1314:1324	thermal degradation	1306:1324	thermal degradation of PLA and PLA composites	1306:1350	The activation energies (Ea) of thermal degradation of PLA and PLA composites were evaluated by Flynn-Wall-Ozawa and Kissinger methods.
24472504	7	63	theme	melting	1090:1096	arg1	peak					1098:1101	unimodal melting peak	1081:1101	unimodal melting peak indicating the α-crystalline form of PLA	1081:1142	Differential scanning calorimetry (DSC) analysis revealed unimodal melting peak indicating the α-crystalline form of PLA.
24472504	3	64	theme	differential	485:496	arg1	analysis					501:508	The differential TG analysis	481:508	The differential TG analysis	481:508	The differential TG analysis revealed that there is no change in the Tmax value (357 °C) for PLA and its composites up to 5 wt% of bio-filler loading.
24472504	6	65	theme	degradation	998:1008	arg1	rate					990:993	the rate	986:993	the rate of degradation	986:1008	This downturn in Tmax at higher loading of the filler is due to the increase in acidic sites and enhancement in the rate of degradation is observed.
24472504	7	66	theme	unimodal	1081:1088	arg1	peak					1098:1101	unimodal melting peak	1081:1101	unimodal melting peak indicating the α-crystalline form of PLA	1081:1142	Differential scanning calorimetry (DSC) analysis revealed unimodal melting peak indicating the α-crystalline form of PLA.
24472504	1	67	theme	"	197:197	arg1	bio-filler					162:171	novel bio-filler	156:171	novel bio-filler	156:171	The current work is focused on investigating the influence of novel bio-filler, "sucrose palmitate (SP)" on the thermal degradation behavior of poly(lactic acid) (PLA) biocomposites in order to render its suitability for food packaging application.
24472504	0	68	theme	lactic	66:71	arg1	acid					73:76	lactic acid	66:76	lactic acid	66:76	Thermal degradation kinetics of sucrose palmitate reinforced poly(lactic acid) biocomposites.
24472504	0	68	theme	lactic	66:71	arg1	poly					61:64	poly	61:64	poly(lactic acid) biocomposites	61:91	Thermal degradation kinetics of sucrose palmitate reinforced poly(lactic acid) biocomposites.
24472504	3	69	theme	Tmax	550:553	arg1	value					555:559	the Tmax value	546:559	the Tmax value (357 °C) for PLA and its composites up to 5 wt% of bio-filler loading	546:629	The differential TG analysis revealed that there is no change in the Tmax value (357 °C) for PLA and its composites up to 5 wt% of bio-filler loading.
24472504	3	69	theme	Tmax	550:553	arg1	°C					566:567	357 °C	562:567	357 °C	562:567	The differential TG analysis revealed that there is no change in the Tmax value (357 °C) for PLA and its composites up to 5 wt% of bio-filler loading.
24472504	1	70	theme	biocomposites	262:274	arg1	behavior					226:233	the thermal degradation behavior	202:233	the thermal degradation behavior of poly(lactic acid) (PLA) biocomposites in order to render its suitability for food packaging application	202:340	The current work is focused on investigating the influence of novel bio-filler, "sucrose palmitate (SP)" on the thermal degradation behavior of poly(lactic acid) (PLA) biocomposites in order to render its suitability for food packaging application.
24472504	5	71	theme	wt	772:773	arg1	%					774:774	10 wt%	769:774	10 wt% of the filler incorporated in the PLA matrix	769:819	In the case of 10 wt% of the filler incorporated in the PLA matrix, Tmax rapidly shifted to lower temperature (324 °C).
24472504	5	71	theme	wt	772:773	arg1	filler					783:788	the filler	779:788	the filler incorporated in the PLA matrix	779:819	In the case of 10 wt% of the filler incorporated in the PLA matrix, Tmax rapidly shifted to lower temperature (324 °C).
24472504	3	72	dep	PLA	574:576	arg1	%					607:607	up to 5 wt%	597:607	up to 5 wt% of bio-filler loading	597:629	The differential TG analysis revealed that there is no change in the Tmax value (357 °C) for PLA and its composites up to 5 wt% of bio-filler loading.
24472504	3	72	dep	PLA	574:576	arg1	loading					623:629	bio-filler loading	612:629	bio-filler loading	612:629	The differential TG analysis revealed that there is no change in the Tmax value (357 °C) for PLA and its composites up to 5 wt% of bio-filler loading.
26944664	7	0	theme	MG	941:942	arg1	dye					944:946	MG dye	941:946	MG dye	941:946	The adsorption followed by photocatalysis and coupled adsorption/photocatalysis reaction achieved about 80% and 90% degradation of MG dye under solar irradiation.
26944664	3	1	theme	ultraviolet-visible	462:480	arg1	spectroscopy					482:493	ultraviolet-visible spectroscopy	462:493	ultraviolet-visible spectroscopy (UV-vis)	462:502	scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), thermal analysis (TGA/DTA), Fourier transform infrared spectroscopy (FTIR) and ultraviolet-visible spectroscopy (UV-vis).
26944664	3	2	theme	transmission	318:329	arg1	TEM					352:354	TEM	352:354	TEM	352:354	scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), thermal analysis (TGA/DTA), Fourier transform infrared spectroscopy (FTIR) and ultraviolet-visible spectroscopy (UV-vis).
26944664	3	2	theme	transmission	318:329	arg1	microscopy					340:349	transmission electron microscopy	318:349	transmission electron microscopy (TEM)	318:355	scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), thermal analysis (TGA/DTA), Fourier transform infrared spectroscopy (FTIR) and ultraviolet-visible spectroscopy (UV-vis).
26944664	3	2	theme	transmission	318:329	arg1	microscopy					300:309	scanning electron microscopy	282:309	scanning electron microscopy (SEM)	282:315	scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), thermal analysis (TGA/DTA), Fourier transform infrared spectroscopy (FTIR) and ultraviolet-visible spectroscopy (UV-vis).
26944664	7	3	theme	%	924:924	arg1	degradation					926:936	90% degradation	922:936	90% degradation of MG dye	922:946	The adsorption followed by photocatalysis and coupled adsorption/photocatalysis reaction achieved about 80% and 90% degradation of MG dye under solar irradiation.
26944664	4	4	theme	composite	544:552	arg1	material					554:561	GG/AO composite material	538:561	GG/AO composite material	538:561	The FTIR analysis confirmed that GG/AO composite material was formed.
26944664	0	5	theme	green	98:102	arg1	degradation					73:83	the degradation	69:83	the degradation of malachite green dye	69:106	Novel guar gum/Al2O3 nanocomposite as an effective photocatalyst for the degradation of malachite green dye.
26944664	8	6	theme	excellent	1003:1011	arg1	activity					1013:1020	the excellent activity	999:1020	the excellent activity of GG/AO nanocomposite against Staphylococcus aureus	999:1073	Antibacterial test showed the excellent activity of GG/AO nanocomposite against Staphylococcus aureus.
26944664	5	7	theme	TEM	575:577	arg1	images					579:584	TEM images	575:584	TEM images	575:584	TEM images inferred the particle size in the range between 20 and 45nm.
26944664	0	8	theme	malachite	88:96	arg1	green					98:102	malachite green dye	88:106	malachite green dye	88:106	Novel guar gum/Al2O3 nanocomposite as an effective photocatalyst for the degradation of malachite green dye.
26944664	6	9	theme	photocatalytic	682:695	arg1	performance					697:707	good photocatalytic performance	677:707	good photocatalytic performance for malachite green (MG) dye (dye initial concentration 1.5×10(-5)M) degradation from aqueous phase	677:807	GG/AO nanocomposite exhibited good photocatalytic performance for malachite green (MG) dye (dye initial concentration 1.5×10(-5)M) degradation from aqueous phase.
26944664	7	10	theme	90	922:923	arg1	%					924:924	%	924:924	%	924:924	The adsorption followed by photocatalysis and coupled adsorption/photocatalysis reaction achieved about 80% and 90% degradation of MG dye under solar irradiation.
26944664	6	11	theme	good	677:680	arg1	performance					697:707	good photocatalytic performance	677:707	good photocatalytic performance for malachite green (MG) dye (dye initial concentration 1.5×10(-5)M) degradation from aqueous phase	677:807	GG/AO nanocomposite exhibited good photocatalytic performance for malachite green (MG) dye (dye initial concentration 1.5×10(-5)M) degradation from aqueous phase.
26944664	5	12	theme	particle	599:606	arg1	size					608:611	the particle size	595:611	the particle size	595:611	TEM images inferred the particle size in the range between 20 and 45nm.
26944664	6	13	theme	concentration	751:763	arg1	M					775:775	dye initial concentration 1.5×10(-5)M	739:775	dye initial concentration 1.5×10(-5)M	739:775	GG/AO nanocomposite exhibited good photocatalytic performance for malachite green (MG) dye (dye initial concentration 1.5×10(-5)M) degradation from aqueous phase.
26944664	6	13	theme	concentration	751:763	arg1	dye					734:736	malachite green (MG) dye	713:736	malachite green (MG) dye (dye initial concentration 1.5×10(-5)M) degradation	713:788	GG/AO nanocomposite exhibited good photocatalytic performance for malachite green (MG) dye (dye initial concentration 1.5×10(-5)M) degradation from aqueous phase.
26944664	1	14	theme	simple	165:170	arg1	method					199:204	simple and cost effective sol-gel method	165:204	simple and cost effective sol-gel method	165:204	Guar gum/Al2O3 (GG/AO) nanocomposite was prepared using simple and cost effective sol-gel method.
26944664	0	15	theme	guar	6:9	arg1	gum/Al2O3					11:19	Novel guar gum/Al2O3	0:19	Novel guar gum/Al2O3	0:19	Novel guar gum/Al2O3 nanocomposite as an effective photocatalyst for the degradation of malachite green dye.
26944664	8	16	theme	nanocomposite	1031:1043	arg1	activity					1013:1020	the excellent activity	999:1020	the excellent activity of GG/AO nanocomposite against Staphylococcus aureus	999:1073	Antibacterial test showed the excellent activity of GG/AO nanocomposite against Staphylococcus aureus.
26944664	6	17	theme	initial	743:749	arg1	M					775:775	dye initial concentration 1.5×10(-5)M	739:775	dye initial concentration 1.5×10(-5)M	739:775	GG/AO nanocomposite exhibited good photocatalytic performance for malachite green (MG) dye (dye initial concentration 1.5×10(-5)M) degradation from aqueous phase.
26944664	6	17	theme	initial	743:749	arg1	dye					734:736	malachite green (MG) dye	713:736	malachite green (MG) dye (dye initial concentration 1.5×10(-5)M) degradation	713:788	GG/AO nanocomposite exhibited good photocatalytic performance for malachite green (MG) dye (dye initial concentration 1.5×10(-5)M) degradation from aqueous phase.
26944664	2	18	dep	techniques	266:275	arg1	viz					277:279	viz	277:279	several analytical techniques viz	247:279	This nanocomposite was characterized by several analytical techniques viz.
26944664	0	19	theme	Novel	0:4	arg1	gum/Al2O3					11:19	Novel guar gum/Al2O3	0:19	Novel guar gum/Al2O3	0:19	Novel guar gum/Al2O3 nanocomposite as an effective photocatalyst for the degradation of malachite green dye.
26944664	8	20	theme	GG/AO	1025:1029	arg1	nanocomposite					1031:1043	GG/AO nanocomposite	1025:1043	GG/AO nanocomposite	1025:1043	Antibacterial test showed the excellent activity of GG/AO nanocomposite against Staphylococcus aureus.
26944664	6	21	theme	dye	739:741	arg1	M					775:775	dye initial concentration 1.5×10(-5)M	739:775	dye initial concentration 1.5×10(-5)M	739:775	GG/AO nanocomposite exhibited good photocatalytic performance for malachite green (MG) dye (dye initial concentration 1.5×10(-5)M) degradation from aqueous phase.
26944664	6	21	theme	dye	739:741	arg1	dye					734:736	malachite green (MG) dye	713:736	malachite green (MG) dye (dye initial concentration 1.5×10(-5)M) degradation	713:788	GG/AO nanocomposite exhibited good photocatalytic performance for malachite green (MG) dye (dye initial concentration 1.5×10(-5)M) degradation from aqueous phase.
26944664	3	22	dep	spectroscopy	482:493	arg1	UV-vis					496:501	UV-vis	496:501	UV-vis	496:501	scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), thermal analysis (TGA/DTA), Fourier transform infrared spectroscopy (FTIR) and ultraviolet-visible spectroscopy (UV-vis).
26944664	6	23	theme	GG/AO	647:651	arg1	nanocomposite					653:665	GG/AO nanocomposite	647:665	GG/AO nanocomposite	647:665	GG/AO nanocomposite exhibited good photocatalytic performance for malachite green (MG) dye (dye initial concentration 1.5×10(-5)M) degradation from aqueous phase.
26944664	3	24	theme	X-ray	358:362	arg1	XRD					377:379	XRD	377:379	XRD	377:379	scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), thermal analysis (TGA/DTA), Fourier transform infrared spectroscopy (FTIR) and ultraviolet-visible spectroscopy (UV-vis).
26944664	3	24	theme	X-ray	358:362	arg1	diffraction					364:374	X-ray diffraction	358:374	X-ray diffraction (XRD)	358:380	scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), thermal analysis (TGA/DTA), Fourier transform infrared spectroscopy (FTIR) and ultraviolet-visible spectroscopy (UV-vis).
26944664	3	24	theme	X-ray	358:362	arg1	microscopy					300:309	scanning electron microscopy	282:309	scanning electron microscopy (SEM)	282:315	scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), thermal analysis (TGA/DTA), Fourier transform infrared spectroscopy (FTIR) and ultraviolet-visible spectroscopy (UV-vis).
26944664	8	25	theme	Antibacterial	973:985	arg1	test					987:990	Antibacterial test	973:990	Antibacterial test	973:990	Antibacterial test showed the excellent activity of GG/AO nanocomposite against Staphylococcus aureus.
26944664	3	26	theme	scanning	282:289	arg1	SEM					312:314	SEM	312:314	SEM	312:314	scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), thermal analysis (TGA/DTA), Fourier transform infrared spectroscopy (FTIR) and ultraviolet-visible spectroscopy (UV-vis).
26944664	3	26	theme	scanning	282:289	arg1	Fourier					411:417	Fourier	411:417	Fourier	411:417	scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), thermal analysis (TGA/DTA), Fourier transform infrared spectroscopy (FTIR) and ultraviolet-visible spectroscopy (UV-vis).
26944664	3	26	theme	scanning	282:289	arg1	analysis					391:398	thermal analysis	383:398	thermal analysis (TGA/DTA)	383:408	scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), thermal analysis (TGA/DTA), Fourier transform infrared spectroscopy (FTIR) and ultraviolet-visible spectroscopy (UV-vis).
26944664	3	26	theme	scanning	282:289	arg1	microscopy					340:349	transmission electron microscopy	318:349	transmission electron microscopy (TEM)	318:355	scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), thermal analysis (TGA/DTA), Fourier transform infrared spectroscopy (FTIR) and ultraviolet-visible spectroscopy (UV-vis).
26944664	3	26	theme	scanning	282:289	arg1	diffraction					364:374	X-ray diffraction	358:374	X-ray diffraction (XRD)	358:380	scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), thermal analysis (TGA/DTA), Fourier transform infrared spectroscopy (FTIR) and ultraviolet-visible spectroscopy (UV-vis).
26944664	3	26	theme	scanning	282:289	arg1	microscopy					300:309	scanning electron microscopy	282:309	scanning electron microscopy (SEM)	282:315	scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), thermal analysis (TGA/DTA), Fourier transform infrared spectroscopy (FTIR) and ultraviolet-visible spectroscopy (UV-vis).
26944664	1	27	theme	effective	181:189	arg1	method					199:204	simple and cost effective sol-gel method	165:204	simple and cost effective sol-gel method	165:204	Guar gum/Al2O3 (GG/AO) nanocomposite was prepared using simple and cost effective sol-gel method.
26944664	6	28	theme	malachite	713:721	arg1	MG					730:731	MG	730:731	MG	730:731	GG/AO nanocomposite exhibited good photocatalytic performance for malachite green (MG) dye (dye initial concentration 1.5×10(-5)M) degradation from aqueous phase.
26944664	6	28	theme	malachite	713:721	arg1	green					723:727	malachite green	713:727	malachite green (MG) dye (dye initial concentration 1.5×10(-5)M) degradation	713:788	GG/AO nanocomposite exhibited good photocatalytic performance for malachite green (MG) dye (dye initial concentration 1.5×10(-5)M) degradation from aqueous phase.
26944664	4	29	theme	GG/AO	538:542	arg1	material					554:561	GG/AO composite material	538:561	GG/AO composite material	538:561	The FTIR analysis confirmed that GG/AO composite material was formed.
26944664	3	30	theme	electron	291:298	arg1	SEM					312:314	SEM	312:314	SEM	312:314	scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), thermal analysis (TGA/DTA), Fourier transform infrared spectroscopy (FTIR) and ultraviolet-visible spectroscopy (UV-vis).
26944664	3	30	theme	electron	291:298	arg1	Fourier					411:417	Fourier	411:417	Fourier	411:417	scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), thermal analysis (TGA/DTA), Fourier transform infrared spectroscopy (FTIR) and ultraviolet-visible spectroscopy (UV-vis).
26944664	3	30	theme	electron	291:298	arg1	analysis					391:398	thermal analysis	383:398	thermal analysis (TGA/DTA)	383:408	scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), thermal analysis (TGA/DTA), Fourier transform infrared spectroscopy (FTIR) and ultraviolet-visible spectroscopy (UV-vis).
26944664	3	30	theme	electron	291:298	arg1	microscopy					340:349	transmission electron microscopy	318:349	transmission electron microscopy (TEM)	318:355	scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), thermal analysis (TGA/DTA), Fourier transform infrared spectroscopy (FTIR) and ultraviolet-visible spectroscopy (UV-vis).
26944664	3	30	theme	electron	291:298	arg1	diffraction					364:374	X-ray diffraction	358:374	X-ray diffraction (XRD)	358:380	scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), thermal analysis (TGA/DTA), Fourier transform infrared spectroscopy (FTIR) and ultraviolet-visible spectroscopy (UV-vis).
26944664	3	30	theme	electron	291:298	arg1	microscopy					300:309	scanning electron microscopy	282:309	scanning electron microscopy (SEM)	282:315	scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), thermal analysis (TGA/DTA), Fourier transform infrared spectroscopy (FTIR) and ultraviolet-visible spectroscopy (UV-vis).
26944664	1	31	theme	sol-gel	191:197	arg1	method					199:204	simple and cost effective sol-gel method	165:204	simple and cost effective sol-gel method	165:204	Guar gum/Al2O3 (GG/AO) nanocomposite was prepared using simple and cost effective sol-gel method.
26944664	3	32	theme	thermal	383:389	arg1	TGA/DTA					401:407	TGA/DTA	401:407	TGA/DTA	401:407	scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), thermal analysis (TGA/DTA), Fourier transform infrared spectroscopy (FTIR) and ultraviolet-visible spectroscopy (UV-vis).
26944664	3	32	theme	thermal	383:389	arg1	microscopy					300:309	scanning electron microscopy	282:309	scanning electron microscopy (SEM)	282:315	scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), thermal analysis (TGA/DTA), Fourier transform infrared spectroscopy (FTIR) and ultraviolet-visible spectroscopy (UV-vis).
26944664	3	32	theme	thermal	383:389	arg1	analysis					391:398	thermal analysis	383:398	thermal analysis (TGA/DTA)	383:408	scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), thermal analysis (TGA/DTA), Fourier transform infrared spectroscopy (FTIR) and ultraviolet-visible spectroscopy (UV-vis).
26944664	6	33	theme	dye	734:736	arg1	degradation					778:788	malachite green (MG) dye (dye initial concentration 1.5×10(-5)M) degradation	713:788	malachite green (MG) dye (dye initial concentration 1.5×10(-5)M) degradation	713:788	GG/AO nanocomposite exhibited good photocatalytic performance for malachite green (MG) dye (dye initial concentration 1.5×10(-5)M) degradation from aqueous phase.
26944664	7	34	theme	dye	944:946	arg1	dye					944:946	MG dye	941:946	MG dye	941:946	The adsorption followed by photocatalysis and coupled adsorption/photocatalysis reaction achieved about 80% and 90% degradation of MG dye under solar irradiation.
26944664	7	34	theme	dye	944:946	arg1	%					916:916	about 80%	908:916	about 80%	908:916	The adsorption followed by photocatalysis and coupled adsorption/photocatalysis reaction achieved about 80% and 90% degradation of MG dye under solar irradiation.
26944664	7	34	theme	dye	944:946	arg1	degradation					926:936	90% degradation	922:936	90% degradation of MG dye	922:946	The adsorption followed by photocatalysis and coupled adsorption/photocatalysis reaction achieved about 80% and 90% degradation of MG dye under solar irradiation.
26944664	6	35	theme	aqueous	795:801	arg1	phase					803:807	aqueous phase	795:807	aqueous phase	795:807	GG/AO nanocomposite exhibited good photocatalytic performance for malachite green (MG) dye (dye initial concentration 1.5×10(-5)M) degradation from aqueous phase.
26944664	1	36	theme	Guar	109:112	arg1	GG/AO					125:129	GG/AO	125:129	GG/AO	125:129	Guar gum/Al2O3 (GG/AO) nanocomposite was prepared using simple and cost effective sol-gel method.
26944664	1	36	theme	Guar	109:112	arg1	gum/Al2O3					114:122	Guar gum/Al2O3	109:122	Guar gum/Al2O3 (GG/AO) nanocomposite	109:144	Guar gum/Al2O3 (GG/AO) nanocomposite was prepared using simple and cost effective sol-gel method.
26944664	0	37	theme	effective	41:49	arg1	photocatalyst					51:63	an effective photocatalyst	38:63	an effective photocatalyst for the degradation of malachite green dye	38:106	Novel guar gum/Al2O3 nanocomposite as an effective photocatalyst for the degradation of malachite green dye.
26944664	4	38	theme	FTIR	509:512	arg1	analysis					514:521	The FTIR analysis	505:521	The FTIR analysis	505:521	The FTIR analysis confirmed that GG/AO composite material was formed.
26944664	7	39	theme	solar	954:958	arg1	irradiation					960:970	solar irradiation	954:970	solar irradiation	954:970	The adsorption followed by photocatalysis and coupled adsorption/photocatalysis reaction achieved about 80% and 90% degradation of MG dye under solar irradiation.
26944664	1	40	theme	gum/Al2O3	114:122	arg1	nanocomposite					132:144	Guar gum/Al2O3 (GG/AO) nanocomposite	109:144	Guar gum/Al2O3 (GG/AO) nanocomposite	109:144	Guar gum/Al2O3 (GG/AO) nanocomposite was prepared using simple and cost effective sol-gel method.
26944664	7	41	theme	adsorption/photocatalysis	864:888	arg1	reaction					890:897	coupled adsorption/photocatalysis reaction	856:897	coupled adsorption/photocatalysis reaction	856:897	The adsorption followed by photocatalysis and coupled adsorption/photocatalysis reaction achieved about 80% and 90% degradation of MG dye under solar irradiation.
26944664	2	42	theme	analytical	255:264	arg1	techniques					266:275	several analytical techniques	247:275	several analytical techniques viz	247:279	This nanocomposite was characterized by several analytical techniques viz.
26944664	6	43	theme	green	723:727	arg1	M					775:775	dye initial concentration 1.5×10(-5)M	739:775	dye initial concentration 1.5×10(-5)M	739:775	GG/AO nanocomposite exhibited good photocatalytic performance for malachite green (MG) dye (dye initial concentration 1.5×10(-5)M) degradation from aqueous phase.
26944664	6	43	theme	green	723:727	arg1	dye					734:736	malachite green (MG) dye	713:736	malachite green (MG) dye (dye initial concentration 1.5×10(-5)M) degradation	713:788	GG/AO nanocomposite exhibited good photocatalytic performance for malachite green (MG) dye (dye initial concentration 1.5×10(-5)M) degradation from aqueous phase.
26944664	3	44	theme	electron	331:338	arg1	TEM					352:354	TEM	352:354	TEM	352:354	scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), thermal analysis (TGA/DTA), Fourier transform infrared spectroscopy (FTIR) and ultraviolet-visible spectroscopy (UV-vis).
26944664	3	44	theme	electron	331:338	arg1	microscopy					340:349	transmission electron microscopy	318:349	transmission electron microscopy (TEM)	318:355	scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), thermal analysis (TGA/DTA), Fourier transform infrared spectroscopy (FTIR) and ultraviolet-visible spectroscopy (UV-vis).
26944664	3	44	theme	electron	331:338	arg1	microscopy					300:309	scanning electron microscopy	282:309	scanning electron microscopy (SEM)	282:315	scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), thermal analysis (TGA/DTA), Fourier transform infrared spectroscopy (FTIR) and ultraviolet-visible spectroscopy (UV-vis).
26944664	2	45	theme	several	247:253	arg1	techniques					266:275	several analytical techniques	247:275	several analytical techniques viz	247:279	This nanocomposite was characterized by several analytical techniques viz.
26944664	3	46	theme	infrared	429:436	arg1	FTIR					452:455	FTIR	452:455	FTIR	452:455	scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), thermal analysis (TGA/DTA), Fourier transform infrared spectroscopy (FTIR) and ultraviolet-visible spectroscopy (UV-vis).
26944664	3	46	theme	infrared	429:436	arg1	spectroscopy					438:449	infrared spectroscopy	429:449	infrared spectroscopy (FTIR)	429:456	scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), thermal analysis (TGA/DTA), Fourier transform infrared spectroscopy (FTIR) and ultraviolet-visible spectroscopy (UV-vis).
26944664	7	47	theme	coupled	856:862	arg1	reaction					890:897	coupled adsorption/photocatalysis reaction	856:897	coupled adsorption/photocatalysis reaction	856:897	The adsorption followed by photocatalysis and coupled adsorption/photocatalysis reaction achieved about 80% and 90% degradation of MG dye under solar irradiation.
26944664	6	48	from	phase	803:807	arg1	performance					697:707	good photocatalytic performance	677:707	good photocatalytic performance for malachite green (MG) dye (dye initial concentration 1.5×10(-5)M) degradation from aqueous phase	677:807	GG/AO nanocomposite exhibited good photocatalytic performance for malachite green (MG) dye (dye initial concentration 1.5×10(-5)M) degradation from aqueous phase.
26919221	4	0	theme	switching	506:514	arg1	layer					516:520	the resistive switching layer	492:520	the resistive switching layer	492:520	The ReRAM behavior can be controlled by mixing starch with chitosan in the resistive switching layer.
26919221	6	1	theme	memory	761:766	arg1	applications					768:779	nonvolatile memory applications	749:779	nonvolatile memory applications	749:779	Both devices exhibit uniform and robust programmable memory properties for nonvolatile memory applications.
26919221	8	2	from	use	1020:1022	arg1	devices					1040:1046	neuromorphic devices	1027:1046	neuromorphic devices	1027:1046	The gradual set/reset behavior in the memory device based on a starch-chitosan mixture makes it suitable for use in neuromorphic devices.
26919221	5	3	theme	memory	610:615	arg1	device					617:622	the memory device	606:622	the memory device with mixed biopolymers	606:645	Whereas starch-based biomemory devices which show abrupt changes in current level; the memory device with mixed biopolymers undergoes gradual changes.
26919221	8	4	theme	gradual	915:921	arg1	behavior					933:940	The gradual set/reset behavior	911:940	The gradual set/reset behavior in the memory device based on a starch-chitosan mixture	911:996	The gradual set/reset behavior in the memory device based on a starch-chitosan mixture makes it suitable for use in neuromorphic devices.
26919221	4	5	theme	ReRAM	425:429	arg1	behavior					431:438	The ReRAM behavior	421:438	The ReRAM behavior	421:438	The ReRAM behavior can be controlled by mixing starch with chitosan in the resistive switching layer.
26919221	6	6	theme	nonvolatile	749:759	arg1	applications					768:779	nonvolatile memory applications	749:779	nonvolatile memory applications	749:779	Both devices exhibit uniform and robust programmable memory properties for nonvolatile memory applications.
26919221	3	7	theme	biomolecular	350:361	arg1	device					370:375	a biomolecular memory device	348:375	a biomolecular memory device that has a starch-chitosan composite layer	348:418	We also introduce a biomolecular memory device that has a starch-chitosan composite layer.
26919221	5	8	theme	mixed	629:633	arg1	biopolymers					635:645	mixed biopolymers	629:645	mixed biopolymers	629:645	Whereas starch-based biomemory devices which show abrupt changes in current level; the memory device with mixed biopolymers undergoes gradual changes.
26919221	2	9	theme	transparent	288:298	arg1	ReRAM					300:304	a robust, nonvolatile, flexible, and transparent ReRAM	251:304	a robust, nonvolatile, flexible, and transparent ReRAM based on potato starch	251:327	We demonstrate a robust, nonvolatile, flexible, and transparent ReRAM based on potato starch.
26919221	8	10	theme	set/reset	923:931	arg1	behavior					933:940	The gradual set/reset behavior	911:940	The gradual set/reset behavior in the memory device based on a starch-chitosan mixture	911:996	The gradual set/reset behavior in the memory device based on a starch-chitosan mixture makes it suitable for use in neuromorphic devices.
26919221	3	11	theme	memory	363:368	arg1	device					370:375	a biomolecular memory device	348:375	a biomolecular memory device that has a starch-chitosan composite layer	348:418	We also introduce a biomolecular memory device that has a starch-chitosan composite layer.
26919221	4	12	theme	resistive	496:504	arg1	layer					516:520	the resistive switching layer	492:520	the resistive switching layer	492:520	The ReRAM behavior can be controlled by mixing starch with chitosan in the resistive switching layer.
26919221	5	13	theme	current	591:597	arg1	level					599:603	current level	591:603	current level	591:603	Whereas starch-based biomemory devices which show abrupt changes in current level; the memory device with mixed biopolymers undergoes gradual changes.
26919221	7	14	theme	filaments	900:908	arg1	rupture					877:883	rupture	877:883	rupture	877:883	The explicated source of the bipolar resistive switching behavior is assigned to formation and rupture of carbon-rich filaments.
26919221	7	14	theme	filaments	900:908	arg1	formation					863:871	formation	863:871	formation	863:871	The explicated source of the bipolar resistive switching behavior is assigned to formation and rupture of carbon-rich filaments.
26919221	3	15	theme	starch-chitosan	388:402	arg1	layer					414:418	a starch-chitosan composite layer	386:418	a starch-chitosan composite layer	386:418	We also introduce a biomolecular memory device that has a starch-chitosan composite layer.
26919221	0	16	from	Behavior	36:43	arg1	Biomemristors					70:82	Starch-Based Flexible Biomemristors	48:82	Starch-Based Flexible Biomemristors	48:82	Controlling the Resistive Switching Behavior in Starch-Based Flexible Biomemristors.
26919221	5	17	theme	gradual	657:663	arg1	changes					665:671	gradual changes	657:671	gradual changes	657:671	Whereas starch-based biomemory devices which show abrupt changes in current level; the memory device with mixed biopolymers undergoes gradual changes.
26919221	3	18	contain	has	382:384	arg1	device					370:375	a biomolecular memory device	348:375	a biomolecular memory device that has a starch-chitosan composite layer	348:418	We also introduce a biomolecular memory device that has a starch-chitosan composite layer.
26919221	3	18	contain	has	382:384	arg2	layer					414:418	a starch-chitosan composite layer	386:418	a starch-chitosan composite layer	386:418	We also introduce a biomolecular memory device that has a starch-chitosan composite layer.
26919221	1	19	theme	biocompatible	103:115	arg1	materials					117:125	biocompatible materials	103:125	biocompatible materials	103:125	Implementation of biocompatible materials in resistive switching memory (ReRAM) devices provides opportunities to use them in biomedical applications.
26919221	6	20	theme	memory	727:732	arg1	properties					734:743	uniform and robust programmable memory properties	695:743	uniform and robust programmable memory properties for nonvolatile memory applications	695:779	Both devices exhibit uniform and robust programmable memory properties for nonvolatile memory applications.
26919221	3	21	theme	composite	404:412	arg1	layer					414:418	a starch-chitosan composite layer	386:418	a starch-chitosan composite layer	386:418	We also introduce a biomolecular memory device that has a starch-chitosan composite layer.
26919221	5	22	from	changes	580:586	arg1	level					599:603	current level	591:603	current level	591:603	Whereas starch-based biomemory devices which show abrupt changes in current level; the memory device with mixed biopolymers undergoes gradual changes.
26919221	4	23	from	chitosan	480:487	arg1	layer					516:520	the resistive switching layer	492:520	the resistive switching layer	492:520	The ReRAM behavior can be controlled by mixing starch with chitosan in the resistive switching layer.
26919221	1	24	theme	materials	117:125	arg1	Implementation					85:98	Implementation	85:98	Implementation of biocompatible materials in resistive switching memory (ReRAM) devices	85:171	Implementation of biocompatible materials in resistive switching memory (ReRAM) devices provides opportunities to use them in biomedical applications.
26919221	6	25	theme	programmable	714:725	arg1	properties					734:743	uniform and robust programmable memory properties	695:743	uniform and robust programmable memory properties for nonvolatile memory applications	695:779	Both devices exhibit uniform and robust programmable memory properties for nonvolatile memory applications.
26919221	0	26	theme	Switching	26:34	arg1	Behavior					36:43	the Resistive Switching Behavior	12:43	the Resistive Switching Behavior in Starch-Based Flexible Biomemristors	12:82	Controlling the Resistive Switching Behavior in Starch-Based Flexible Biomemristors.
26919221	2	27	theme	potato	315:320	arg1	starch					322:327	potato starch	315:327	potato starch	315:327	We demonstrate a robust, nonvolatile, flexible, and transparent ReRAM based on potato starch.
26919221	6	28	theme	robust	707:712	arg1	properties					734:743	uniform and robust programmable memory properties	695:743	uniform and robust programmable memory properties for nonvolatile memory applications	695:779	Both devices exhibit uniform and robust programmable memory properties for nonvolatile memory applications.
26919221	1	29	theme	biomedical	211:220	arg1	applications					222:233	biomedical applications	211:233	biomedical applications	211:233	Implementation of biocompatible materials in resistive switching memory (ReRAM) devices provides opportunities to use them in biomedical applications.
26919221	0	30	theme	Resistive	16:24	arg1	Behavior					36:43	the Resistive Switching Behavior	12:43	the Resistive Switching Behavior in Starch-Based Flexible Biomemristors	12:82	Controlling the Resistive Switching Behavior in Starch-Based Flexible Biomemristors.
26919221	5	31	dep	undergoes	647:655	arg1	devices					554:560	starch-based biomemory devices	531:560	starch-based biomemory devices which show abrupt changes in current level	531:603	Whereas starch-based biomemory devices which show abrupt changes in current level; the memory device with mixed biopolymers undergoes gradual changes.
26919221	5	31	dep	undergoes	647:655	arg1	Whereas					523:529	Whereas	523:529	Whereas	523:529	Whereas starch-based biomemory devices which show abrupt changes in current level; the memory device with mixed biopolymers undergoes gradual changes.
26919221	1	32	theme	resistive	130:138	arg1	devices					165:171	resistive switching memory (ReRAM) devices	130:171	resistive switching memory (ReRAM) devices	130:171	Implementation of biocompatible materials in resistive switching memory (ReRAM) devices provides opportunities to use them in biomedical applications.
26919221	8	33	theme	neuromorphic	1027:1038	arg1	devices					1040:1046	neuromorphic devices	1027:1046	neuromorphic devices	1027:1046	The gradual set/reset behavior in the memory device based on a starch-chitosan mixture makes it suitable for use in neuromorphic devices.
26919221	6	34	theme	uniform	695:701	arg1	properties					734:743	uniform and robust programmable memory properties	695:743	uniform and robust programmable memory properties for nonvolatile memory applications	695:779	Both devices exhibit uniform and robust programmable memory properties for nonvolatile memory applications.
26919221	7	35	theme	switching	829:837	arg1	behavior					839:846	the bipolar resistive switching behavior	807:846	the bipolar resistive switching behavior	807:846	The explicated source of the bipolar resistive switching behavior is assigned to formation and rupture of carbon-rich filaments.
26919221	2	36	theme	robust	253:258	arg1	ReRAM					300:304	a robust, nonvolatile, flexible, and transparent ReRAM	251:304	a robust, nonvolatile, flexible, and transparent ReRAM based on potato starch	251:327	We demonstrate a robust, nonvolatile, flexible, and transparent ReRAM based on potato starch.
26919221	8	37	theme	starch-chitosan	974:988	arg1	mixture					990:996	a starch-chitosan mixture	972:996	a starch-chitosan mixture	972:996	The gradual set/reset behavior in the memory device based on a starch-chitosan mixture makes it suitable for use in neuromorphic devices.
26919221	7	38	theme	resistive	819:827	arg1	behavior					839:846	the bipolar resistive switching behavior	807:846	the bipolar resistive switching behavior	807:846	The explicated source of the bipolar resistive switching behavior is assigned to formation and rupture of carbon-rich filaments.
26919221	0	39	theme	Flexible	61:68	arg1	Biomemristors					70:82	Starch-Based Flexible Biomemristors	48:82	Starch-Based Flexible Biomemristors	48:82	Controlling the Resistive Switching Behavior in Starch-Based Flexible Biomemristors.
26919221	7	40	theme	behavior	839:846	arg1	source					797:802	The explicated source	782:802	The explicated source of the bipolar resistive switching behavior	782:846	The explicated source of the bipolar resistive switching behavior is assigned to formation and rupture of carbon-rich filaments.
26919221	7	41	theme	carbon-rich	888:898	arg1	filaments					900:908	carbon-rich filaments	888:908	carbon-rich filaments	888:908	The explicated source of the bipolar resistive switching behavior is assigned to formation and rupture of carbon-rich filaments.
26919221	8	42	from	behavior	933:940	arg1	device					956:961	the memory device	945:961	the memory device based on a starch-chitosan mixture	945:996	The gradual set/reset behavior in the memory device based on a starch-chitosan mixture makes it suitable for use in neuromorphic devices.
26919221	5	43	theme	abrupt	573:578	arg1	changes					580:586	abrupt changes	573:586	abrupt changes in current level	573:603	Whereas starch-based biomemory devices which show abrupt changes in current level; the memory device with mixed biopolymers undergoes gradual changes.
26919221	1	44	theme	switching	140:148	arg1	devices					165:171	resistive switching memory (ReRAM) devices	130:171	resistive switching memory (ReRAM) devices	130:171	Implementation of biocompatible materials in resistive switching memory (ReRAM) devices provides opportunities to use them in biomedical applications.
26919221	0	45	theme	Starch-Based	48:59	arg1	Biomemristors					70:82	Starch-Based Flexible Biomemristors	48:82	Starch-Based Flexible Biomemristors	48:82	Controlling the Resistive Switching Behavior in Starch-Based Flexible Biomemristors.
26919221	7	46	theme	bipolar	811:817	arg1	behavior					839:846	the bipolar resistive switching behavior	807:846	the bipolar resistive switching behavior	807:846	The explicated source of the bipolar resistive switching behavior is assigned to formation and rupture of carbon-rich filaments.
26919221	1	47	theme	memory	150:155	arg1	devices					165:171	resistive switching memory (ReRAM) devices	130:171	resistive switching memory (ReRAM) devices	130:171	Implementation of biocompatible materials in resistive switching memory (ReRAM) devices provides opportunities to use them in biomedical applications.
26919221	5	48	with	device	617:622	arg1	biopolymers					635:645	mixed biopolymers	629:645	mixed biopolymers	629:645	Whereas starch-based biomemory devices which show abrupt changes in current level; the memory device with mixed biopolymers undergoes gradual changes.
26919221	2	49	theme	flexible	274:281	arg1	ReRAM					300:304	a robust, nonvolatile, flexible, and transparent ReRAM	251:304	a robust, nonvolatile, flexible, and transparent ReRAM based on potato starch	251:327	We demonstrate a robust, nonvolatile, flexible, and transparent ReRAM based on potato starch.
26919221	5	50	theme	starch-based	531:542	arg1	devices					554:560	starch-based biomemory devices	531:560	starch-based biomemory devices which show abrupt changes in current level	531:603	Whereas starch-based biomemory devices which show abrupt changes in current level; the memory device with mixed biopolymers undergoes gradual changes.
26919221	1	51	theme	ReRAM	158:162	arg1	devices					165:171	resistive switching memory (ReRAM) devices	130:171	resistive switching memory (ReRAM) devices	130:171	Implementation of biocompatible materials in resistive switching memory (ReRAM) devices provides opportunities to use them in biomedical applications.
26919221	5	52	theme	biomemory	544:552	arg1	devices					554:560	starch-based biomemory devices	531:560	starch-based biomemory devices which show abrupt changes in current level	531:603	Whereas starch-based biomemory devices which show abrupt changes in current level; the memory device with mixed biopolymers undergoes gradual changes.
26919221	7	53	theme	explicated	786:795	arg1	source					797:802	The explicated source	782:802	The explicated source of the bipolar resistive switching behavior	782:846	The explicated source of the bipolar resistive switching behavior is assigned to formation and rupture of carbon-rich filaments.
26919221	8	54	theme	memory	949:954	arg1	device					956:961	the memory device	945:961	the memory device based on a starch-chitosan mixture	945:996	The gradual set/reset behavior in the memory device based on a starch-chitosan mixture makes it suitable for use in neuromorphic devices.
26919221	2	55	theme	nonvolatile	261:271	arg1	ReRAM					300:304	a robust, nonvolatile, flexible, and transparent ReRAM	251:304	a robust, nonvolatile, flexible, and transparent ReRAM based on potato starch	251:327	We demonstrate a robust, nonvolatile, flexible, and transparent ReRAM based on potato starch.
26919221	1	56	from	Implementation	85:98	arg1	devices					165:171	resistive switching memory (ReRAM) devices	130:171	resistive switching memory (ReRAM) devices	130:171	Implementation of biocompatible materials in resistive switching memory (ReRAM) devices provides opportunities to use them in biomedical applications.
25463236	0	0	theme	Pseudomonas	80:90	arg1	ZJU1					103:106	Pseudomonas aeruginosa ZJU1	80:106	Pseudomonas aeruginosa ZJU1	80:106	Preparation of a new-style composite containing a key bioflocculant produced by Pseudomonas aeruginosa ZJU1 and its flocculating effect on harmful algal blooms.
25463236	2	1	theme	isolated	435:442	arg1	ZJU1					467:470	a newly isolated Pseudomonas aeruginosa ZJU1	427:470	a newly isolated Pseudomonas aeruginosa ZJU1	427:470	The extracellular polymeric substances (EPS), produced with a yield of 3.58±0.11 g/L by a newly isolated Pseudomonas aeruginosa ZJU1, was indicated to be a key component in the composite.
25463236	3	2	theme	nucleic	640:646	arg1	components					667:676	the main components	658:676	the main components; polar functional groups in the EPS are responsible for its flocculating activity	658:758	The components and functional groups of the EPS were analyzed, and it showed that polysaccharides, proteins, and nucleic acids are the main components; polar functional groups in the EPS are responsible for its flocculating activity.
25463236	3	2	theme	nucleic	640:646	arg1	proteins					626:633	proteins	626:633	proteins	626:633	The components and functional groups of the EPS were analyzed, and it showed that polysaccharides, proteins, and nucleic acids are the main components; polar functional groups in the EPS are responsible for its flocculating activity.
25463236	3	2	theme	nucleic	640:646	arg1	polysaccharides					609:623	polysaccharides	609:623	polysaccharides	609:623	The components and functional groups of the EPS were analyzed, and it showed that polysaccharides, proteins, and nucleic acids are the main components; polar functional groups in the EPS are responsible for its flocculating activity.
25463236	3	2	theme	nucleic	640:646	arg1	acids					648:652	nucleic acids	640:652	nucleic acids	640:652	The components and functional groups of the EPS were analyzed, and it showed that polysaccharides, proteins, and nucleic acids are the main components; polar functional groups in the EPS are responsible for its flocculating activity.
25463236	3	3	theme	flocculating	738:749	arg1	activity					751:758	its flocculating activity	734:758	its flocculating activity	734:758	The components and functional groups of the EPS were analyzed, and it showed that polysaccharides, proteins, and nucleic acids are the main components; polar functional groups in the EPS are responsible for its flocculating activity.
25463236	4	4	theme	composite	898:906	arg1	contents					882:889	contents	882:889	contents	882:889	The novel composite was optimized by the response surface methodology and after optimization, the optical components and contents of the composite were Kaolin 2.38 g/L, CaCl2 0.28 g/L, KAl(SO4)2 0.09 g/L, and EPS 1.75 mg/L.
25463236	4	4	theme	composite	898:906	arg1	components					867:876	optical components	859:876	optical components	859:876	The novel composite was optimized by the response surface methodology and after optimization, the optical components and contents of the composite were Kaolin 2.38 g/L, CaCl2 0.28 g/L, KAl(SO4)2 0.09 g/L, and EPS 1.75 mg/L.
25463236	3	5	dep	components	667:676	arg1	responsible					718:728	responsible	718:728	responsible	718:728	The components and functional groups of the EPS were analyzed, and it showed that polysaccharides, proteins, and nucleic acids are the main components; polar functional groups in the EPS are responsible for its flocculating activity.
25463236	2	6	from	component	499:507	arg1	composite					516:524	the composite	512:524	the composite	512:524	The extracellular polymeric substances (EPS), produced with a yield of 3.58±0.11 g/L by a newly isolated Pseudomonas aeruginosa ZJU1, was indicated to be a key component in the composite.
25463236	3	7	from	groups	696:701	arg1	EPS					710:712	the EPS	706:712	the EPS	706:712	The components and functional groups of the EPS were analyzed, and it showed that polysaccharides, proteins, and nucleic acids are the main components; polar functional groups in the EPS are responsible for its flocculating activity.
25463236	5	8	dep	1.0	1213:1215	arg1	to					1210:1211	to	1210:1211	to	1210:1211	The flocculating rates of the composite were tested, and it could rapidly reach 100±0.13% within 2 min when OD680 of Microcystis aeruginosa was 0.1; it could reach 100±0.08% within 5 min for OD680 of M. aeruginosa in HABs up to 1.0.
25463236	4	9	theme	optical	859:865	arg1	components					867:876	optical components	859:876	optical components	859:876	The novel composite was optimized by the response surface methodology and after optimization, the optical components and contents of the composite were Kaolin 2.38 g/L, CaCl2 0.28 g/L, KAl(SO4)2 0.09 g/L, and EPS 1.75 mg/L.
25463236	6	10	theme	HABs	1322:1325	arg1	treatment					1309:1317	the treatment	1305:1317	the treatment of HABs caused by M. aeruginosa	1305:1349	These results suggest that the novel composite will be a highly efficient material for the treatment of HABs caused by M. aeruginosa.
25463236	4	11	dep	KAl	946:948	arg1	g/L					961:963	0.09 g/L	956:963	KAl(SO4)2 0.09 g/L	946:963	The novel composite was optimized by the response surface methodology and after optimization, the optical components and contents of the composite were Kaolin 2.38 g/L, CaCl2 0.28 g/L, KAl(SO4)2 0.09 g/L, and EPS 1.75 mg/L.
25463236	3	12	dep	components	531:540	arg1	The					527:529	The	527:529	The	527:529	The components and functional groups of the EPS were analyzed, and it showed that polysaccharides, proteins, and nucleic acids are the main components; polar functional groups in the EPS are responsible for its flocculating activity.
25463236	1	13	theme	novel	163:167	arg1	composite					169:177	A novel composite	161:177	A novel composite consisting of clay, bioflocculant, and inorganic flocculant	161:237	A novel composite consisting of clay, bioflocculant, and inorganic flocculant was designed, and its flocculating effect on harmful algal blooms (HABs) was studied in this study.
25463236	5	14	theme	composite	1015:1023	arg1	rates					1002:1006	The flocculating rates	985:1006	The flocculating rates of the composite	985:1023	The flocculating rates of the composite were tested, and it could rapidly reach 100±0.13% within 2 min when OD680 of Microcystis aeruginosa was 0.1; it could reach 100±0.08% within 5 min for OD680 of M. aeruginosa in HABs up to 1.0.
25463236	3	15	theme	main	662:665	arg1	components					667:676	the main components	658:676	the main components; polar functional groups in the EPS are responsible for its flocculating activity	658:758	The components and functional groups of the EPS were analyzed, and it showed that polysaccharides, proteins, and nucleic acids are the main components; polar functional groups in the EPS are responsible for its flocculating activity.
25463236	3	15	theme	main	662:665	arg1	proteins					626:633	proteins	626:633	proteins	626:633	The components and functional groups of the EPS were analyzed, and it showed that polysaccharides, proteins, and nucleic acids are the main components; polar functional groups in the EPS are responsible for its flocculating activity.
25463236	3	15	theme	main	662:665	arg1	polysaccharides					609:623	polysaccharides	609:623	polysaccharides	609:623	The components and functional groups of the EPS were analyzed, and it showed that polysaccharides, proteins, and nucleic acids are the main components; polar functional groups in the EPS are responsible for its flocculating activity.
25463236	3	15	theme	main	662:665	arg1	acids					648:652	nucleic acids	640:652	nucleic acids	640:652	The components and functional groups of the EPS were analyzed, and it showed that polysaccharides, proteins, and nucleic acids are the main components; polar functional groups in the EPS are responsible for its flocculating activity.
25463236	0	16	dep	Pseudomonas	80:90	arg1	aeruginosa					92:101	aeruginosa	92:101	aeruginosa	92:101	Preparation of a new-style composite containing a key bioflocculant produced by Pseudomonas aeruginosa ZJU1 and its flocculating effect on harmful algal blooms.
25463236	4	17	theme	response	802:809	arg1	methodology					819:829	the response surface methodology	798:829	the response surface methodology	798:829	The novel composite was optimized by the response surface methodology and after optimization, the optical components and contents of the composite were Kaolin 2.38 g/L, CaCl2 0.28 g/L, KAl(SO4)2 0.09 g/L, and EPS 1.75 mg/L.
25463236	2	18	theme	polymeric	357:365	arg1	substances					367:376	The extracellular polymeric substances	339:376	The extracellular polymeric substances (EPS)	339:382	The extracellular polymeric substances (EPS), produced with a yield of 3.58±0.11 g/L by a newly isolated Pseudomonas aeruginosa ZJU1, was indicated to be a key component in the composite.
25463236	2	18	theme	polymeric	357:365	arg1	component					499:507	a key component	493:507	a key component	493:507	The extracellular polymeric substances (EPS), produced with a yield of 3.58±0.11 g/L by a newly isolated Pseudomonas aeruginosa ZJU1, was indicated to be a key component in the composite.
25463236	2	18	theme	polymeric	357:365	arg1	EPS					379:381	EPS	379:381	EPS	379:381	The extracellular polymeric substances (EPS), produced with a yield of 3.58±0.11 g/L by a newly isolated Pseudomonas aeruginosa ZJU1, was indicated to be a key component in the composite.
25463236	4	19	theme	CaCl2	930:934	arg1	g/L					925:927	Kaolin 2.38 g/L	913:927	Kaolin 2.38 g/L	913:927	The novel composite was optimized by the response surface methodology and after optimization, the optical components and contents of the composite were Kaolin 2.38 g/L, CaCl2 0.28 g/L, KAl(SO4)2 0.09 g/L, and EPS 1.75 mg/L.
25463236	4	19	theme	CaCl2	930:934	arg1	g/L					941:943	CaCl2 0.28 g/L	930:943	CaCl2 0.28 g/L	930:943	The novel composite was optimized by the response surface methodology and after optimization, the optical components and contents of the composite were Kaolin 2.38 g/L, CaCl2 0.28 g/L, KAl(SO4)2 0.09 g/L, and EPS 1.75 mg/L.
25463236	1	20	theme	flocculating	261:272	arg1	effect					274:279	its flocculating effect	257:279	its flocculating effect on harmful algal blooms (HABs)	257:310	A novel composite consisting of clay, bioflocculant, and inorganic flocculant was designed, and its flocculating effect on harmful algal blooms (HABs) was studied in this study.
25463236	1	21	from	effect	274:279	arg1	blooms					298:303	harmful algal blooms	284:303	harmful algal blooms (HABs)	284:310	A novel composite consisting of clay, bioflocculant, and inorganic flocculant was designed, and its flocculating effect on harmful algal blooms (HABs) was studied in this study.
25463236	1	21	from	effect	274:279	arg1	HABs					306:309	HABs	306:309	HABs	306:309	A novel composite consisting of clay, bioflocculant, and inorganic flocculant was designed, and its flocculating effect on harmful algal blooms (HABs) was studied in this study.
25463236	4	22	theme	EPS	970:972	arg1	mg/L					979:982	EPS 1.75 mg/L	970:982	EPS 1.75 mg/L	970:982	The novel composite was optimized by the response surface methodology and after optimization, the optical components and contents of the composite were Kaolin 2.38 g/L, CaCl2 0.28 g/L, KAl(SO4)2 0.09 g/L, and EPS 1.75 mg/L.
25463236	2	23	theme	extracellular	343:355	arg1	substances					367:376	The extracellular polymeric substances	339:376	The extracellular polymeric substances (EPS)	339:382	The extracellular polymeric substances (EPS), produced with a yield of 3.58±0.11 g/L by a newly isolated Pseudomonas aeruginosa ZJU1, was indicated to be a key component in the composite.
25463236	2	23	theme	extracellular	343:355	arg1	component					499:507	a key component	493:507	a key component	493:507	The extracellular polymeric substances (EPS), produced with a yield of 3.58±0.11 g/L by a newly isolated Pseudomonas aeruginosa ZJU1, was indicated to be a key component in the composite.
25463236	2	23	theme	extracellular	343:355	arg1	EPS					379:381	EPS	379:381	EPS	379:381	The extracellular polymeric substances (EPS), produced with a yield of 3.58±0.11 g/L by a newly isolated Pseudomonas aeruginosa ZJU1, was indicated to be a key component in the composite.
25463236	0	24	theme	new-style	17:25	arg1	composite					27:35	a new-style composite	15:35	a new-style composite	15:35	Preparation of a new-style composite containing a key bioflocculant produced by Pseudomonas aeruginosa ZJU1 and its flocculating effect on harmful algal blooms.
25463236	2	25	theme	key	495:497	arg1	substances					367:376	The extracellular polymeric substances	339:376	The extracellular polymeric substances (EPS)	339:382	The extracellular polymeric substances (EPS), produced with a yield of 3.58±0.11 g/L by a newly isolated Pseudomonas aeruginosa ZJU1, was indicated to be a key component in the composite.
25463236	2	25	theme	key	495:497	arg1	component					499:507	a key component	493:507	a key component	493:507	The extracellular polymeric substances (EPS), produced with a yield of 3.58±0.11 g/L by a newly isolated Pseudomonas aeruginosa ZJU1, was indicated to be a key component in the composite.
25463236	0	26	theme	flocculating	116:127	arg1	effect					129:134	its flocculating effect	112:134	its flocculating effect on harmful algal blooms	112:158	Preparation of a new-style composite containing a key bioflocculant produced by Pseudomonas aeruginosa ZJU1 and its flocculating effect on harmful algal blooms.
25463236	2	27	theme	g/L	420:422	arg1	yield					401:405	a yield	399:405	a yield of 3.58±0.11 g/L	399:422	The extracellular polymeric substances (EPS), produced with a yield of 3.58±0.11 g/L by a newly isolated Pseudomonas aeruginosa ZJU1, was indicated to be a key component in the composite.
25463236	1	28	theme	harmful	284:290	arg1	blooms					298:303	harmful algal blooms	284:303	harmful algal blooms (HABs)	284:310	A novel composite consisting of clay, bioflocculant, and inorganic flocculant was designed, and its flocculating effect on harmful algal blooms (HABs) was studied in this study.
25463236	1	28	theme	harmful	284:290	arg1	HABs					306:309	HABs	306:309	HABs	306:309	A novel composite consisting of clay, bioflocculant, and inorganic flocculant was designed, and its flocculating effect on harmful algal blooms (HABs) was studied in this study.
25463236	0	29	theme	harmful	139:145	arg1	blooms					153:158	harmful algal blooms	139:158	harmful algal blooms	139:158	Preparation of a new-style composite containing a key bioflocculant produced by Pseudomonas aeruginosa ZJU1 and its flocculating effect on harmful algal blooms.
25463236	5	30	from	OD680	1176:1180	arg1	HABs					1202:1205	HABs up to 1.0	1202:1215	HABs up to 1.0	1202:1215	The flocculating rates of the composite were tested, and it could rapidly reach 100±0.13% within 2 min when OD680 of Microcystis aeruginosa was 0.1; it could reach 100±0.08% within 5 min for OD680 of M. aeruginosa in HABs up to 1.0.
25463236	0	31	theme	composite	27:35	arg1	Preparation					0:10	Preparation	0:10	Preparation of a new-style composite	0:35	Preparation of a new-style composite containing a key bioflocculant produced by Pseudomonas aeruginosa ZJU1 and its flocculating effect on harmful algal blooms.
25463236	1	32	theme	algal	292:296	arg1	blooms					298:303	harmful algal blooms	284:303	harmful algal blooms (HABs)	284:310	A novel composite consisting of clay, bioflocculant, and inorganic flocculant was designed, and its flocculating effect on harmful algal blooms (HABs) was studied in this study.
25463236	1	32	theme	algal	292:296	arg1	HABs					306:309	HABs	306:309	HABs	306:309	A novel composite consisting of clay, bioflocculant, and inorganic flocculant was designed, and its flocculating effect on harmful algal blooms (HABs) was studied in this study.
25463236	4	33	theme	Kaolin	913:918	arg1	g/L					925:927	Kaolin 2.38 g/L	913:927	Kaolin 2.38 g/L	913:927	The novel composite was optimized by the response surface methodology and after optimization, the optical components and contents of the composite were Kaolin 2.38 g/L, CaCl2 0.28 g/L, KAl(SO4)2 0.09 g/L, and EPS 1.75 mg/L.
25463236	4	33	theme	Kaolin	913:918	arg1	g/L					941:943	CaCl2 0.28 g/L	930:943	CaCl2 0.28 g/L	930:943	The novel composite was optimized by the response surface methodology and after optimization, the optical components and contents of the composite were Kaolin 2.38 g/L, CaCl2 0.28 g/L, KAl(SO4)2 0.09 g/L, and EPS 1.75 mg/L.
25463236	3	34	theme	EPS	571:573	arg1	components					531:540	components	531:540	components	531:540	The components and functional groups of the EPS were analyzed, and it showed that polysaccharides, proteins, and nucleic acids are the main components; polar functional groups in the EPS are responsible for its flocculating activity.
25463236	3	34	theme	EPS	571:573	arg1	groups					557:562	functional groups	546:562	functional groups	546:562	The components and functional groups of the EPS were analyzed, and it showed that polysaccharides, proteins, and nucleic acids are the main components; polar functional groups in the EPS are responsible for its flocculating activity.
25463236	3	34	theme	EPS	571:573	arg1	EPS					571:573	the EPS	567:573	the EPS	567:573	The components and functional groups of the EPS were analyzed, and it showed that polysaccharides, proteins, and nucleic acids are the main components; polar functional groups in the EPS are responsible for its flocculating activity.
25463236	5	35	theme	aeruginosa	1188:1197	arg1	OD680					1176:1180	OD680	1176:1180	OD680 of M. aeruginosa in HABs up to 1.0	1176:1215	The flocculating rates of the composite were tested, and it could rapidly reach 100±0.13% within 2 min when OD680 of Microcystis aeruginosa was 0.1; it could reach 100±0.08% within 5 min for OD680 of M. aeruginosa in HABs up to 1.0.
25463236	0	36	theme	key	50:52	arg1	bioflocculant					54:66	a key bioflocculant	48:66	a key bioflocculant produced by Pseudomonas aeruginosa ZJU1 and its flocculating effect on harmful algal blooms	48:158	Preparation of a new-style composite containing a key bioflocculant produced by Pseudomonas aeruginosa ZJU1 and its flocculating effect on harmful algal blooms.
25463236	4	37	theme	surface	811:817	arg1	methodology					819:829	the response surface methodology	798:829	the response surface methodology	798:829	The novel composite was optimized by the response surface methodology and after optimization, the optical components and contents of the composite were Kaolin 2.38 g/L, CaCl2 0.28 g/L, KAl(SO4)2 0.09 g/L, and EPS 1.75 mg/L.
25463236	4	38	theme	novel	765:769	arg1	composite					771:779	The novel composite	761:779	The novel composite	761:779	The novel composite was optimized by the response surface methodology and after optimization, the optical components and contents of the composite were Kaolin 2.38 g/L, CaCl2 0.28 g/L, KAl(SO4)2 0.09 g/L, and EPS 1.75 mg/L.
25463236	5	39	theme	flocculating	989:1000	arg1	rates					1002:1006	The flocculating rates	985:1006	The flocculating rates of the composite	985:1023	The flocculating rates of the composite were tested, and it could rapidly reach 100±0.13% within 2 min when OD680 of Microcystis aeruginosa was 0.1; it could reach 100±0.08% within 5 min for OD680 of M. aeruginosa in HABs up to 1.0.
25463236	3	40	theme	polar	679:683	arg1	groups					696:701	polar functional groups	679:701	polar functional groups in the EPS	679:712	The components and functional groups of the EPS were analyzed, and it showed that polysaccharides, proteins, and nucleic acids are the main components; polar functional groups in the EPS are responsible for its flocculating activity.
25463236	0	41	theme	algal	147:151	arg1	blooms					153:158	harmful algal blooms	139:158	harmful algal blooms	139:158	Preparation of a new-style composite containing a key bioflocculant produced by Pseudomonas aeruginosa ZJU1 and its flocculating effect on harmful algal blooms.
25463236	5	42	theme	aeruginosa	1114:1123	arg1	OD680					1093:1097	OD680	1093:1097	OD680 of Microcystis aeruginosa	1093:1123	The flocculating rates of the composite were tested, and it could rapidly reach 100±0.13% within 2 min when OD680 of Microcystis aeruginosa was 0.1; it could reach 100±0.08% within 5 min for OD680 of M. aeruginosa in HABs up to 1.0.
25463236	3	43	theme	functional	685:694	arg1	groups					696:701	polar functional groups	679:701	polar functional groups in the EPS	679:712	The components and functional groups of the EPS were analyzed, and it showed that polysaccharides, proteins, and nucleic acids are the main components; polar functional groups in the EPS are responsible for its flocculating activity.
25463236	6	44	theme	efficient	1282:1290	arg1	composite					1255:1263	the novel composite	1245:1263	the novel composite	1245:1263	These results suggest that the novel composite will be a highly efficient material for the treatment of HABs caused by M. aeruginosa.
25463236	6	44	theme	efficient	1282:1290	arg1	material					1292:1299	a highly efficient material	1273:1299	a highly efficient material for the treatment of HABs caused by M. aeruginosa	1273:1349	These results suggest that the novel composite will be a highly efficient material for the treatment of HABs caused by M. aeruginosa.
25463236	2	45	dep	Pseudomonas	444:454	arg1	aeruginosa					456:465	aeruginosa	456:465	aeruginosa	456:465	The extracellular polymeric substances (EPS), produced with a yield of 3.58±0.11 g/L by a newly isolated Pseudomonas aeruginosa ZJU1, was indicated to be a key component in the composite.
25463236	0	46	from	ZJU1	103:106	arg1	blooms					153:158	harmful algal blooms	139:158	harmful algal blooms	139:158	Preparation of a new-style composite containing a key bioflocculant produced by Pseudomonas aeruginosa ZJU1 and its flocculating effect on harmful algal blooms.
25463236	4	47	dep	components	867:876	arg1	the					855:857	the	855:857	the	855:857	The novel composite was optimized by the response surface methodology and after optimization, the optical components and contents of the composite were Kaolin 2.38 g/L, CaCl2 0.28 g/L, KAl(SO4)2 0.09 g/L, and EPS 1.75 mg/L.
25463236	1	48	theme	inorganic	218:226	arg1	flocculant					228:237	inorganic flocculant	218:237	inorganic flocculant	218:237	A novel composite consisting of clay, bioflocculant, and inorganic flocculant was designed, and its flocculating effect on harmful algal blooms (HABs) was studied in this study.
25463236	6	49	theme	novel	1249:1253	arg1	composite					1255:1263	the novel composite	1245:1263	the novel composite	1245:1263	These results suggest that the novel composite will be a highly efficient material for the treatment of HABs caused by M. aeruginosa.
25463236	6	49	theme	novel	1249:1253	arg1	material					1292:1299	a highly efficient material	1273:1299	a highly efficient material for the treatment of HABs caused by M. aeruginosa	1273:1349	These results suggest that the novel composite will be a highly efficient material for the treatment of HABs caused by M. aeruginosa.
25463236	3	50	theme	functional	546:555	arg1	groups					557:562	functional groups	546:562	functional groups	546:562	The components and functional groups of the EPS were analyzed, and it showed that polysaccharides, proteins, and nucleic acids are the main components; polar functional groups in the EPS are responsible for its flocculating activity.
25463236	3	50	theme	functional	546:555	arg1	EPS					571:573	the EPS	567:573	the EPS	567:573	The components and functional groups of the EPS were analyzed, and it showed that polysaccharides, proteins, and nucleic acids are the main components; polar functional groups in the EPS are responsible for its flocculating activity.
25463236	0	51	from	effect	129:134	arg1	blooms					153:158	harmful algal blooms	139:158	harmful algal blooms	139:158	Preparation of a new-style composite containing a key bioflocculant produced by Pseudomonas aeruginosa ZJU1 and its flocculating effect on harmful algal blooms.
25463236	2	52	theme	Pseudomonas	444:454	arg1	ZJU1					467:470	a newly isolated Pseudomonas aeruginosa ZJU1	427:470	a newly isolated Pseudomonas aeruginosa ZJU1	427:470	The extracellular polymeric substances (EPS), produced with a yield of 3.58±0.11 g/L by a newly isolated Pseudomonas aeruginosa ZJU1, was indicated to be a key component in the composite.
27987966	0	0	theme	elastic	72:78	arg1	ionogels					80:87	highly conductive and elastic ionogels	50:87	highly conductive and elastic ionogels with poly(ionic liquid) and ionic liquid	50:128	Guar gum as biosourced building block to generate highly conductive and elastic ionogels with poly(ionic liquid) and ionic liquid.
27987966	3	1	theme	combined	648:655	arg1	presence					657:664	The combined presence	644:664	The combined presence of guar gum and PIL	644:684	The combined presence of guar gum and PIL confers excellent dimensional stability to the ionogels with no IL exudation combined with high thermal properties (up to 310°C).
27987966	2	2	theme	biohybrid	535:543	arg1	ionogels					545:552	physical biohybrid ionogels	526:552	physical biohybrid ionogels	526:552	The development of intermolecular polar interactions (mainly hydrogen bonds) and topologic chain entanglements induces the formation of physical biohybrid ionogels, whose elastic properties can be easily tuned by varying the composition (up to 30000Pa).
27987966	5	3	dep	good	1023:1026	arg1	compromise					1028:1037	compromise	1028:1037	compromise	1028:1037	The benefit linked to the formation of guar/PIL associations in IL medium enables to find a good compromise between the mechanical cohesion and the mobility ensuring the ionic transport.
27987966	1	4	theme	ionogels	205:212	arg1	preparation					190:200	the simple and straightforward preparation	159:200	the simple and straightforward preparation of ionogels arising from the addition of guar gum (a plant-based polysaccharide) in a solution of precisely-defined poly(ionic liquid) chains (PIL) in imidazolium-based ionic liquid (IL)	159:387	In this study, we report on the simple and straightforward preparation of ionogels arising from the addition of guar gum (a plant-based polysaccharide) in a solution of precisely-defined poly(ionic liquid) chains (PIL) in imidazolium-based ionic liquid (IL).
27987966	2	5	dep	interactions	430:441	arg1	bonds					460:464	hydrogen bonds	451:464	mainly hydrogen bonds	444:464	The development of intermolecular polar interactions (mainly hydrogen bonds) and topologic chain entanglements induces the formation of physical biohybrid ionogels, whose elastic properties can be easily tuned by varying the composition (up to 30000Pa).
27987966	2	6	theme	physical	526:533	arg1	ionogels					545:552	physical biohybrid ionogels	526:552	physical biohybrid ionogels	526:552	The development of intermolecular polar interactions (mainly hydrogen bonds) and topologic chain entanglements induces the formation of physical biohybrid ionogels, whose elastic properties can be easily tuned by varying the composition (up to 30000Pa).
27987966	3	7	theme	high	777:780	arg1	properties					790:799	high thermal properties	777:799	high thermal properties (up to 310°C)	777:813	The combined presence of guar gum and PIL confers excellent dimensional stability to the ionogels with no IL exudation combined with high thermal properties (up to 310°C).
27987966	2	8	theme	hydrogen	451:458	arg1	bonds					460:464	hydrogen bonds	451:464	mainly hydrogen bonds	444:464	The development of intermolecular polar interactions (mainly hydrogen bonds) and topologic chain entanglements induces the formation of physical biohybrid ionogels, whose elastic properties can be easily tuned by varying the composition (up to 30000Pa).
27987966	4	9	dep	conductivities	894:907	arg1	>					910:910	>	910:910	> 10-4S/cm at 30°C	910:927	The resulting materials are shown to exhibit gel scattering profiles and high conductivities (> 10-4S/cm at 30°C).
27987966	5	10	theme	mechanical	1051:1060	arg1	cohesion					1062:1069	the mechanical cohesion	1047:1069	the mechanical cohesion	1047:1069	The benefit linked to the formation of guar/PIL associations in IL medium enables to find a good compromise between the mechanical cohesion and the mobility ensuring the ionic transport.
27987966	3	11	theme	thermal	782:788	arg1	properties					790:799	high thermal properties	777:799	high thermal properties (up to 310°C)	777:813	The combined presence of guar gum and PIL confers excellent dimensional stability to the ionogels with no IL exudation combined with high thermal properties (up to 310°C).
27987966	1	12	from	chains	337:342	arg1	liquid					377:382	liquid	377:382	liquid	377:382	In this study, we report on the simple and straightforward preparation of ionogels arising from the addition of guar gum (a plant-based polysaccharide) in a solution of precisely-defined poly(ionic liquid) chains (PIL) in imidazolium-based ionic liquid (IL).
27987966	1	12	from	chains	337:342	arg1	IL					385:386	IL	385:386	IL	385:386	In this study, we report on the simple and straightforward preparation of ionogels arising from the addition of guar gum (a plant-based polysaccharide) in a solution of precisely-defined poly(ionic liquid) chains (PIL) in imidazolium-based ionic liquid (IL).
27987966	5	13	theme	guar/PIL	970:977	arg1	associations					979:990	guar/PIL associations	970:990	guar/PIL associations	970:990	The benefit linked to the formation of guar/PIL associations in IL medium enables to find a good compromise between the mechanical cohesion and the mobility ensuring the ionic transport.
27987966	4	14	theme	high	889:892	arg1	conductivities					894:907	high conductivities	889:907	high conductivities (> 10-4S/cm at 30°C)	889:928	The resulting materials are shown to exhibit gel scattering profiles and high conductivities (> 10-4S/cm at 30°C).
27987966	1	15	theme	precisely-defined	300:316	arg1	poly					318:321	precisely-defined poly	300:321	precisely-defined poly(ionic liquid) chains (PIL) in imidazolium-based ionic liquid (IL)	300:387	In this study, we report on the simple and straightforward preparation of ionogels arising from the addition of guar gum (a plant-based polysaccharide) in a solution of precisely-defined poly(ionic liquid) chains (PIL) in imidazolium-based ionic liquid (IL).
27987966	2	16	theme	elastic	561:567	arg1	properties					569:578	elastic properties	561:578	elastic properties	561:578	The development of intermolecular polar interactions (mainly hydrogen bonds) and topologic chain entanglements induces the formation of physical biohybrid ionogels, whose elastic properties can be easily tuned by varying the composition (up to 30000Pa).
27987966	2	17	theme	interactions	430:441	arg1	development					394:404	The development	390:404	The development of intermolecular polar interactions (mainly hydrogen bonds) and topologic chain entanglements	390:499	The development of intermolecular polar interactions (mainly hydrogen bonds) and topologic chain entanglements induces the formation of physical biohybrid ionogels, whose elastic properties can be easily tuned by varying the composition (up to 30000Pa).
27987966	1	18	theme	poly	318:321	arg1	PIL					345:347	PIL	345:347	PIL	345:347	In this study, we report on the simple and straightforward preparation of ionogels arising from the addition of guar gum (a plant-based polysaccharide) in a solution of precisely-defined poly(ionic liquid) chains (PIL) in imidazolium-based ionic liquid (IL).
27987966	1	18	theme	poly	318:321	arg1	chains					337:342	precisely-defined poly(ionic liquid) chains	300:342	precisely-defined poly(ionic liquid) chains (PIL) in imidazolium-based ionic liquid (IL)	300:387	In this study, we report on the simple and straightforward preparation of ionogels arising from the addition of guar gum (a plant-based polysaccharide) in a solution of precisely-defined poly(ionic liquid) chains (PIL) in imidazolium-based ionic liquid (IL).
27987966	1	19	from	gum	248:250	arg1	solution					288:295	a solution	286:295	a solution of precisely-defined poly(ionic liquid) chains (PIL) in imidazolium-based ionic liquid (IL)	286:387	In this study, we report on the simple and straightforward preparation of ionogels arising from the addition of guar gum (a plant-based polysaccharide) in a solution of precisely-defined poly(ionic liquid) chains (PIL) in imidazolium-based ionic liquid (IL).
27987966	3	20	theme	gum	674:676	arg1	presence					657:664	The combined presence	644:664	The combined presence of guar gum and PIL	644:684	The combined presence of guar gum and PIL confers excellent dimensional stability to the ionogels with no IL exudation combined with high thermal properties (up to 310°C).
27987966	5	21	theme	IL	995:996	arg1	medium					998:1003	IL medium	995:1003	IL medium	995:1003	The benefit linked to the formation of guar/PIL associations in IL medium enables to find a good compromise between the mechanical cohesion and the mobility ensuring the ionic transport.
27987966	1	22	from	addition	231:238	arg1	solution					288:295	a solution	286:295	a solution of precisely-defined poly(ionic liquid) chains (PIL) in imidazolium-based ionic liquid (IL)	286:387	In this study, we report on the simple and straightforward preparation of ionogels arising from the addition of guar gum (a plant-based polysaccharide) in a solution of precisely-defined poly(ionic liquid) chains (PIL) in imidazolium-based ionic liquid (IL).
27987966	2	23	theme	polar	424:428	arg1	interactions					430:441	intermolecular polar interactions	409:441	intermolecular polar interactions (mainly hydrogen bonds)	409:465	The development of intermolecular polar interactions (mainly hydrogen bonds) and topologic chain entanglements induces the formation of physical biohybrid ionogels, whose elastic properties can be easily tuned by varying the composition (up to 30000Pa).
27987966	5	24	theme	associations	979:990	arg1	formation					957:965	the formation	953:965	the formation of guar/PIL associations in IL medium	953:1003	The benefit linked to the formation of guar/PIL associations in IL medium enables to find a good compromise between the mechanical cohesion and the mobility ensuring the ionic transport.
27987966	0	25	theme	Guar	0:3	arg1	gum					5:7	Guar gum	0:7	Guar gum	0:7	Guar gum as biosourced building block to generate highly conductive and elastic ionogels with poly(ionic liquid) and ionic liquid.
27987966	2	26	theme	ionogels	545:552	arg1	formation					513:521	the formation	509:521	the formation of physical biohybrid ionogels, whose elastic properties can be easily tuned by varying the composition (up to 30000Pa)	509:641	The development of intermolecular polar interactions (mainly hydrogen bonds) and topologic chain entanglements induces the formation of physical biohybrid ionogels, whose elastic properties can be easily tuned by varying the composition (up to 30000Pa).
27987966	3	27	dep	properties	790:799	arg1	310°C					808:812	310°C	808:812	310°C	808:812	The combined presence of guar gum and PIL confers excellent dimensional stability to the ionogels with no IL exudation combined with high thermal properties (up to 310°C).
27987966	3	28	theme	guar	669:672	arg1	gum					674:676	guar gum	669:676	guar gum	669:676	The combined presence of guar gum and PIL confers excellent dimensional stability to the ionogels with no IL exudation combined with high thermal properties (up to 310°C).
27987966	0	29	theme	liquid	105:110	arg1	poly					94:97	poly(ionic liquid) and ionic liquid	94:128	poly	94:97	Guar gum as biosourced building block to generate highly conductive and elastic ionogels with poly(ionic liquid) and ionic liquid.
27987966	0	30	with	ionogels	80:87	arg1	liquid					123:128	liquid	123:128	liquid	123:128	Guar gum as biosourced building block to generate highly conductive and elastic ionogels with poly(ionic liquid) and ionic liquid.
27987966	0	30	with	ionogels	80:87	arg1	poly					94:97	poly(ionic liquid) and ionic liquid	94:128	poly	94:97	Guar gum as biosourced building block to generate highly conductive and elastic ionogels with poly(ionic liquid) and ionic liquid.
27987966	1	31	theme	guar	243:246	arg1	gum					248:250	guar gum	243:250	guar gum (a plant-based polysaccharide) in a solution of precisely-defined poly(ionic liquid) chains (PIL) in imidazolium-based ionic liquid (IL)	243:387	In this study, we report on the simple and straightforward preparation of ionogels arising from the addition of guar gum (a plant-based polysaccharide) in a solution of precisely-defined poly(ionic liquid) chains (PIL) in imidazolium-based ionic liquid (IL).
27987966	1	31	theme	guar	243:246	arg1	polysaccharide					267:280	a plant-based polysaccharide	253:280	a plant-based polysaccharide	253:280	In this study, we report on the simple and straightforward preparation of ionogels arising from the addition of guar gum (a plant-based polysaccharide) in a solution of precisely-defined poly(ionic liquid) chains (PIL) in imidazolium-based ionic liquid (IL).
27987966	0	32	theme	ionic	99:103	arg1	poly					94:97	poly(ionic liquid) and ionic liquid	94:128	poly	94:97	Guar gum as biosourced building block to generate highly conductive and elastic ionogels with poly(ionic liquid) and ionic liquid.
27987966	4	33	theme	scattering	865:874	arg1	profiles					876:883	gel scattering profiles	861:883	gel scattering profiles	861:883	The resulting materials are shown to exhibit gel scattering profiles and high conductivities (> 10-4S/cm at 30°C).
27987966	1	34	theme	gum	248:250	arg1	addition					231:238	the addition	227:238	the addition of guar gum (a plant-based polysaccharide) in a solution of precisely-defined poly(ionic liquid) chains (PIL) in imidazolium-based ionic liquid (IL)	227:387	In this study, we report on the simple and straightforward preparation of ionogels arising from the addition of guar gum (a plant-based polysaccharide) in a solution of precisely-defined poly(ionic liquid) chains (PIL) in imidazolium-based ionic liquid (IL).
27987966	5	35	attach	linked	943:948	arg1	formation					957:965	the formation	953:965	the formation of guar/PIL associations in IL medium	953:1003	The benefit linked to the formation of guar/PIL associations in IL medium enables to find a good compromise between the mechanical cohesion and the mobility ensuring the ionic transport.
27987966	5	35	attach	linked	943:948	arg2	benefit					935:941	The benefit	931:941	The benefit linked to the formation of guar/PIL associations in IL medium	931:1003	The benefit linked to the formation of guar/PIL associations in IL medium enables to find a good compromise between the mechanical cohesion and the mobility ensuring the ionic transport.
27987966	0	36	theme	building	23:30	arg1	block					32:36	building block	23:36	building block	23:36	Guar gum as biosourced building block to generate highly conductive and elastic ionogels with poly(ionic liquid) and ionic liquid.
27987966	1	37	dep	poly	318:321	arg1	liquid					329:334	liquid	329:334	liquid	329:334	In this study, we report on the simple and straightforward preparation of ionogels arising from the addition of guar gum (a plant-based polysaccharide) in a solution of precisely-defined poly(ionic liquid) chains (PIL) in imidazolium-based ionic liquid (IL).
27987966	1	38	from	solution	288:295	arg1	liquid					377:382	liquid	377:382	liquid	377:382	In this study, we report on the simple and straightforward preparation of ionogels arising from the addition of guar gum (a plant-based polysaccharide) in a solution of precisely-defined poly(ionic liquid) chains (PIL) in imidazolium-based ionic liquid (IL).
27987966	1	38	from	solution	288:295	arg1	addition					231:238	the addition	227:238	the addition of guar gum (a plant-based polysaccharide) in a solution of precisely-defined poly(ionic liquid) chains (PIL) in imidazolium-based ionic liquid (IL)	227:387	In this study, we report on the simple and straightforward preparation of ionogels arising from the addition of guar gum (a plant-based polysaccharide) in a solution of precisely-defined poly(ionic liquid) chains (PIL) in imidazolium-based ionic liquid (IL).
27987966	1	38	from	solution	288:295	arg1	IL					385:386	IL	385:386	IL	385:386	In this study, we report on the simple and straightforward preparation of ionogels arising from the addition of guar gum (a plant-based polysaccharide) in a solution of precisely-defined poly(ionic liquid) chains (PIL) in imidazolium-based ionic liquid (IL).
27987966	1	39	theme	simple	163:168	arg1	preparation					190:200	the simple and straightforward preparation	159:200	the simple and straightforward preparation of ionogels arising from the addition of guar gum (a plant-based polysaccharide) in a solution of precisely-defined poly(ionic liquid) chains (PIL) in imidazolium-based ionic liquid (IL)	159:387	In this study, we report on the simple and straightforward preparation of ionogels arising from the addition of guar gum (a plant-based polysaccharide) in a solution of precisely-defined poly(ionic liquid) chains (PIL) in imidazolium-based ionic liquid (IL).
27987966	3	40	theme	PIL	682:684	arg1	presence					657:664	The combined presence	644:664	The combined presence of guar gum and PIL	644:684	The combined presence of guar gum and PIL confers excellent dimensional stability to the ionogels with no IL exudation combined with high thermal properties (up to 310°C).
27987966	5	41	from	formation	957:965	arg1	medium					998:1003	IL medium	995:1003	IL medium	995:1003	The benefit linked to the formation of guar/PIL associations in IL medium enables to find a good compromise between the mechanical cohesion and the mobility ensuring the ionic transport.
27987966	1	42	theme	chains	337:342	arg1	solution					288:295	a solution	286:295	a solution of precisely-defined poly(ionic liquid) chains (PIL) in imidazolium-based ionic liquid (IL)	286:387	In this study, we report on the simple and straightforward preparation of ionogels arising from the addition of guar gum (a plant-based polysaccharide) in a solution of precisely-defined poly(ionic liquid) chains (PIL) in imidazolium-based ionic liquid (IL).
27987966	3	43	with	ionogels	733:740	arg1	exudation					753:761	no IL exudation	747:761	no IL exudation combined with high thermal properties (up to 310°C)	747:813	The combined presence of guar gum and PIL confers excellent dimensional stability to the ionogels with no IL exudation combined with high thermal properties (up to 310°C).
27987966	4	44	theme	resulting	820:828	arg1	materials					830:838	The resulting materials	816:838	The resulting materials	816:838	The resulting materials are shown to exhibit gel scattering profiles and high conductivities (> 10-4S/cm at 30°C).
27987966	1	45	theme	plant-based	255:265	arg1	gum					248:250	guar gum	243:250	guar gum (a plant-based polysaccharide) in a solution of precisely-defined poly(ionic liquid) chains (PIL) in imidazolium-based ionic liquid (IL)	243:387	In this study, we report on the simple and straightforward preparation of ionogels arising from the addition of guar gum (a plant-based polysaccharide) in a solution of precisely-defined poly(ionic liquid) chains (PIL) in imidazolium-based ionic liquid (IL).
27987966	1	45	theme	plant-based	255:265	arg1	polysaccharide					267:280	a plant-based polysaccharide	253:280	a plant-based polysaccharide	253:280	In this study, we report on the simple and straightforward preparation of ionogels arising from the addition of guar gum (a plant-based polysaccharide) in a solution of precisely-defined poly(ionic liquid) chains (PIL) in imidazolium-based ionic liquid (IL).
27987966	2	46	theme	topologic	471:479	arg1	entanglements					487:499	topologic chain entanglements	471:499	topologic chain entanglements	471:499	The development of intermolecular polar interactions (mainly hydrogen bonds) and topologic chain entanglements induces the formation of physical biohybrid ionogels, whose elastic properties can be easily tuned by varying the composition (up to 30000Pa).
27987966	4	47	theme	gel	861:863	arg1	profiles					876:883	gel scattering profiles	861:883	gel scattering profiles	861:883	The resulting materials are shown to exhibit gel scattering profiles and high conductivities (> 10-4S/cm at 30°C).
27987966	3	48	dep	310°C	808:812	arg1	to					805:806	to	805:806	to	805:806	The combined presence of guar gum and PIL confers excellent dimensional stability to the ionogels with no IL exudation combined with high thermal properties (up to 310°C).
27987966	2	49	theme	entanglements	487:499	arg1	development					394:404	The development	390:404	The development of intermolecular polar interactions (mainly hydrogen bonds) and topologic chain entanglements	390:499	The development of intermolecular polar interactions (mainly hydrogen bonds) and topologic chain entanglements induces the formation of physical biohybrid ionogels, whose elastic properties can be easily tuned by varying the composition (up to 30000Pa).
27987966	5	50	theme	ionic	1101:1105	arg1	transport					1107:1115	the ionic transport	1097:1115	the ionic transport	1097:1115	The benefit linked to the formation of guar/PIL associations in IL medium enables to find a good compromise between the mechanical cohesion and the mobility ensuring the ionic transport.
27987966	2	51	theme	chain	481:485	arg1	entanglements					487:499	topologic chain entanglements	471:499	topologic chain entanglements	471:499	The development of intermolecular polar interactions (mainly hydrogen bonds) and topologic chain entanglements induces the formation of physical biohybrid ionogels, whose elastic properties can be easily tuned by varying the composition (up to 30000Pa).
27987966	3	52	theme	IL	750:751	arg1	exudation					753:761	no IL exudation	747:761	no IL exudation combined with high thermal properties (up to 310°C)	747:813	The combined presence of guar gum and PIL confers excellent dimensional stability to the ionogels with no IL exudation combined with high thermal properties (up to 310°C).
27987966	1	53	theme	straightforward	174:188	arg1	preparation					190:200	the simple and straightforward preparation	159:200	the simple and straightforward preparation of ionogels arising from the addition of guar gum (a plant-based polysaccharide) in a solution of precisely-defined poly(ionic liquid) chains (PIL) in imidazolium-based ionic liquid (IL)	159:387	In this study, we report on the simple and straightforward preparation of ionogels arising from the addition of guar gum (a plant-based polysaccharide) in a solution of precisely-defined poly(ionic liquid) chains (PIL) in imidazolium-based ionic liquid (IL).
27987966	0	54	theme	conductive	57:66	arg1	ionogels					80:87	highly conductive and elastic ionogels	50:87	highly conductive and elastic ionogels with poly(ionic liquid) and ionic liquid	50:128	Guar gum as biosourced building block to generate highly conductive and elastic ionogels with poly(ionic liquid) and ionic liquid.
27987966	1	55	from	liquid	377:382	arg1	solution					288:295	a solution	286:295	a solution of precisely-defined poly(ionic liquid) chains (PIL) in imidazolium-based ionic liquid (IL)	286:387	In this study, we report on the simple and straightforward preparation of ionogels arising from the addition of guar gum (a plant-based polysaccharide) in a solution of precisely-defined poly(ionic liquid) chains (PIL) in imidazolium-based ionic liquid (IL).
27987966	3	56	theme	excellent	694:702	arg1	stability					716:724	excellent dimensional stability	694:724	excellent dimensional stability	694:724	The combined presence of guar gum and PIL confers excellent dimensional stability to the ionogels with no IL exudation combined with high thermal properties (up to 310°C).
27987966	2	57	theme	intermolecular	409:422	arg1	interactions					430:441	intermolecular polar interactions	409:441	intermolecular polar interactions (mainly hydrogen bonds)	409:465	The development of intermolecular polar interactions (mainly hydrogen bonds) and topologic chain entanglements induces the formation of physical biohybrid ionogels, whose elastic properties can be easily tuned by varying the composition (up to 30000Pa).
27987966	3	58	theme	dimensional	704:714	arg1	stability					716:724	excellent dimensional stability	694:724	excellent dimensional stability	694:724	The combined presence of guar gum and PIL confers excellent dimensional stability to the ionogels with no IL exudation combined with high thermal properties (up to 310°C).
27992794	0	0	theme	Dried	75:79	arg1	Grains					81:86	Distillers Dried Grains	64:86	Distillers Dried Grains with Solubles composition	64:112	A comparison between corn and grain sorghum fermentation rates, Distillers Dried Grains with Solubles composition, and lipid profiles.
27992794	4	1	with	Grains	724:729	arg1	Solubles					736:743	Solubles	736:743	Solubles produced from sorghum	736:765	Distillers Dried Grains with Solubles produced from sorghum had a statistically significant higher yields and significantly higher protein content relative to corn.
27992794	0	2	theme	Distillers	64:73	arg1	Grains					81:86	Distillers Dried Grains	64:86	Distillers Dried Grains with Solubles composition	64:112	A comparison between corn and grain sorghum fermentation rates, Distillers Dried Grains with Solubles composition, and lipid profiles.
27992794	4	3	theme	Dried	718:722	arg1	Grains					724:729	Distillers Dried Grains	707:729	Distillers Dried Grains with Solubles produced from sorghum	707:765	Distillers Dried Grains with Solubles produced from sorghum had a statistically significant higher yields and significantly higher protein content relative to corn.
27992794	1	4	theme	corn	232:235	arg1	ethanol					244:250	corn impact ethanol	232:250	corn impact ethanol	232:250	The aim of this study was to determine if the compositional difference between grain sorghum and corn impact ethanol yields and coproduct value when grain sorghum is incorporated into existing corn ethanol facilities.
27992794	1	5	theme	corn	328:331	arg1	facilities					341:350	corn ethanol facilities	328:350	corn ethanol facilities	328:350	The aim of this study was to determine if the compositional difference between grain sorghum and corn impact ethanol yields and coproduct value when grain sorghum is incorporated into existing corn ethanol facilities.
27992794	4	6	theme	higher	799:804	arg1	yields					806:811	higher yields	799:811	a statistically significant higher yields	771:811	Distillers Dried Grains with Solubles produced from sorghum had a statistically significant higher yields and significantly higher protein content relative to corn.
27992794	1	7	theme	impact	237:242	arg1	ethanol					244:250	corn impact ethanol	232:250	corn impact ethanol	232:250	The aim of this study was to determine if the compositional difference between grain sorghum and corn impact ethanol yields and coproduct value when grain sorghum is incorporated into existing corn ethanol facilities.
27992794	1	8	theme	ethanol	333:339	arg1	facilities					341:350	corn ethanol facilities	328:350	corn ethanol facilities	328:350	The aim of this study was to determine if the compositional difference between grain sorghum and corn impact ethanol yields and coproduct value when grain sorghum is incorporated into existing corn ethanol facilities.
27992794	4	9	theme	significant	787:797	arg1	yields					806:811	higher yields	799:811	a statistically significant higher yields	771:811	Distillers Dried Grains with Solubles produced from sorghum had a statistically significant higher yields and significantly higher protein content relative to corn.
27992794	3	10	theme	protease	561:568	arg1	addition					570:577	protease addition	561:577	protease addition	561:577	Fermentation results indicated that protease addition influenced the fermentation rate and yield for grain sorghum, improving yields by 1-2% over non-protease treated fermentations.
27992794	5	11	theme	Distillers	894:903	arg1	Grains					911:916	the Distillers Dried Grains	890:916	the Distillers Dried Grains with Solubles	890:930	Lipid analysis of the Distillers Dried Grains with Solubles showed statistically significant differences between corn and sorghum in triacylglycerol, diacylglycerol and free fatty acid levels.
27992794	0	12	with	rates	57:61	arg1	composition					102:112	Solubles composition	93:112	Solubles composition	93:112	A comparison between corn and grain sorghum fermentation rates, Distillers Dried Grains with Solubles composition, and lipid profiles.
27992794	5	13	theme	free	1041:1044	arg1	acid					1052:1055	free fatty acid	1041:1055	free fatty acid	1041:1055	Lipid analysis of the Distillers Dried Grains with Solubles showed statistically significant differences between corn and sorghum in triacylglycerol, diacylglycerol and free fatty acid levels.
27992794	3	14	dep	rate	607:610	arg1	the					590:592	the	590:592	the	590:592	Fermentation results indicated that protease addition influenced the fermentation rate and yield for grain sorghum, improving yields by 1-2% over non-protease treated fermentations.
27992794	0	15	theme	Solubles	93:100	arg1	composition					102:112	Solubles composition	93:112	Solubles composition	93:112	A comparison between corn and grain sorghum fermentation rates, Distillers Dried Grains with Solubles composition, and lipid profiles.
27992794	0	16	theme	grain	30:34	arg1	rates					57:61	corn and grain sorghum fermentation rates	21:61	corn and grain sorghum fermentation rates	21:61	A comparison between corn and grain sorghum fermentation rates, Distillers Dried Grains with Solubles composition, and lipid profiles.
27992794	5	17	theme	fatty	1046:1050	arg1	acid					1052:1055	free fatty acid	1041:1055	free fatty acid	1041:1055	Lipid analysis of the Distillers Dried Grains with Solubles showed statistically significant differences between corn and sorghum in triacylglycerol, diacylglycerol and free fatty acid levels.
27992794	1	18	theme	study	151:155	arg1	aim					139:141	The aim	135:141	The aim of this study	135:155	The aim of this study was to determine if the compositional difference between grain sorghum and corn impact ethanol yields and coproduct value when grain sorghum is incorporated into existing corn ethanol facilities.
27992794	2	19	dep	systems	444:450	arg1	liquefaction					481:492	conventional thermal starch liquefaction	453:492	conventional thermal starch liquefaction	453:492	Fermentation properties of corn and grain sorghum were compared utilizing two fermentation systems (conventional thermal starch liquefaction and native starch hydrolysis).
27992794	2	19	dep	systems	444:450	arg1	systems					444:450	two fermentation systems	427:450	two fermentation systems (conventional thermal starch liquefaction and native starch hydrolysis)	427:522	Fermentation properties of corn and grain sorghum were compared utilizing two fermentation systems (conventional thermal starch liquefaction and native starch hydrolysis).
27992794	2	19	dep	systems	444:450	arg1	hydrolysis					512:521	native starch hydrolysis	498:521	native starch hydrolysis	498:521	Fermentation properties of corn and grain sorghum were compared utilizing two fermentation systems (conventional thermal starch liquefaction and native starch hydrolysis).
27992794	5	20	theme	Grains	911:916	arg1	analysis					878:885	Lipid analysis	872:885	Lipid analysis of the Distillers Dried Grains with Solubles	872:930	Lipid analysis of the Distillers Dried Grains with Solubles showed statistically significant differences between corn and sorghum in triacylglycerol, diacylglycerol and free fatty acid levels.
27992794	2	21	theme	fermentation	431:442	arg1	liquefaction					481:492	conventional thermal starch liquefaction	453:492	conventional thermal starch liquefaction	453:492	Fermentation properties of corn and grain sorghum were compared utilizing two fermentation systems (conventional thermal starch liquefaction and native starch hydrolysis).
27992794	2	21	theme	fermentation	431:442	arg1	systems					444:450	two fermentation systems	427:450	two fermentation systems (conventional thermal starch liquefaction and native starch hydrolysis)	427:522	Fermentation properties of corn and grain sorghum were compared utilizing two fermentation systems (conventional thermal starch liquefaction and native starch hydrolysis).
27992794	2	21	theme	fermentation	431:442	arg1	hydrolysis					512:521	native starch hydrolysis	498:521	native starch hydrolysis	498:521	Fermentation properties of corn and grain sorghum were compared utilizing two fermentation systems (conventional thermal starch liquefaction and native starch hydrolysis).
27992794	3	22	theme	non-protease	671:682	arg1	fermentations					692:704	non-protease treated fermentations	671:704	non-protease treated fermentations	671:704	Fermentation results indicated that protease addition influenced the fermentation rate and yield for grain sorghum, improving yields by 1-2% over non-protease treated fermentations.
27992794	5	23	from	differences	965:975	arg1	levels					1057:1062	triacylglycerol, diacylglycerol and free fatty acid levels	1005:1062	triacylglycerol, diacylglycerol and free fatty acid levels	1005:1062	Lipid analysis of the Distillers Dried Grains with Solubles showed statistically significant differences between corn and sorghum in triacylglycerol, diacylglycerol and free fatty acid levels.
27992794	3	24	theme	fermentation	594:605	arg1	rate					607:610	fermentation rate	594:610	fermentation rate	594:610	Fermentation results indicated that protease addition influenced the fermentation rate and yield for grain sorghum, improving yields by 1-2% over non-protease treated fermentations.
27992794	0	25	theme	corn	21:24	arg1	rates					57:61	corn and grain sorghum fermentation rates	21:61	corn and grain sorghum fermentation rates	21:61	A comparison between corn and grain sorghum fermentation rates, Distillers Dried Grains with Solubles composition, and lipid profiles.
27992794	0	26	with	Grains	81:86	arg1	composition					102:112	Solubles composition	93:112	Solubles composition	93:112	A comparison between corn and grain sorghum fermentation rates, Distillers Dried Grains with Solubles composition, and lipid profiles.
27992794	5	27	theme	triacylglycerol	1005:1019	arg1	levels					1057:1062	triacylglycerol, diacylglycerol and free fatty acid levels	1005:1062	triacylglycerol, diacylglycerol and free fatty acid levels	1005:1062	Lipid analysis of the Distillers Dried Grains with Solubles showed statistically significant differences between corn and sorghum in triacylglycerol, diacylglycerol and free fatty acid levels.
27992794	5	28	theme	Lipid	872:876	arg1	analysis					878:885	Lipid analysis	872:885	Lipid analysis of the Distillers Dried Grains with Solubles	872:930	Lipid analysis of the Distillers Dried Grains with Solubles showed statistically significant differences between corn and sorghum in triacylglycerol, diacylglycerol and free fatty acid levels.
27992794	1	29	theme	coproduct	263:271	arg1	value					273:277	and coproduct value	259:277	value	273:277	The aim of this study was to determine if the compositional difference between grain sorghum and corn impact ethanol yields and coproduct value when grain sorghum is incorporated into existing corn ethanol facilities.
27992794	5	30	theme	significant	953:963	arg1	differences					965:975	statistically significant differences	939:975	statistically significant differences between corn and sorghum in triacylglycerol, diacylglycerol and free fatty acid levels	939:1062	Lipid analysis of the Distillers Dried Grains with Solubles showed statistically significant differences between corn and sorghum in triacylglycerol, diacylglycerol and free fatty acid levels.
27992794	4	31	theme	Distillers	707:716	arg1	Grains					724:729	Distillers Dried Grains	707:729	Distillers Dried Grains with Solubles produced from sorghum	707:765	Distillers Dried Grains with Solubles produced from sorghum had a statistically significant higher yields and significantly higher protein content relative to corn.
27992794	2	32	theme	starch	474:479	arg1	liquefaction					481:492	conventional thermal starch liquefaction	453:492	conventional thermal starch liquefaction	453:492	Fermentation properties of corn and grain sorghum were compared utilizing two fermentation systems (conventional thermal starch liquefaction and native starch hydrolysis).
27992794	2	32	theme	starch	474:479	arg1	systems					444:450	two fermentation systems	427:450	two fermentation systems (conventional thermal starch liquefaction and native starch hydrolysis)	427:522	Fermentation properties of corn and grain sorghum were compared utilizing two fermentation systems (conventional thermal starch liquefaction and native starch hydrolysis).
27992794	4	33	contain	had	767:769	arg1	Grains					724:729	Distillers Dried Grains	707:729	Distillers Dried Grains with Solubles produced from sorghum	707:765	Distillers Dried Grains with Solubles produced from sorghum had a statistically significant higher yields and significantly higher protein content relative to corn.
27992794	4	33	contain	had	767:769	arg2	content					846:852	significantly higher protein content	817:852	significantly higher protein content	817:852	Distillers Dried Grains with Solubles produced from sorghum had a statistically significant higher yields and significantly higher protein content relative to corn.
27992794	4	33	contain	had	767:769	arg2	yields					806:811	higher yields	799:811	a statistically significant higher yields	771:811	Distillers Dried Grains with Solubles produced from sorghum had a statistically significant higher yields and significantly higher protein content relative to corn.
27992794	2	34	theme	sorghum	395:401	arg1	properties					366:375	Fermentation properties	353:375	Fermentation properties of corn and grain sorghum	353:401	Fermentation properties of corn and grain sorghum were compared utilizing two fermentation systems (conventional thermal starch liquefaction and native starch hydrolysis).
27992794	2	35	theme	thermal	466:472	arg1	liquefaction					481:492	conventional thermal starch liquefaction	453:492	conventional thermal starch liquefaction	453:492	Fermentation properties of corn and grain sorghum were compared utilizing two fermentation systems (conventional thermal starch liquefaction and native starch hydrolysis).
27992794	2	35	theme	thermal	466:472	arg1	systems					444:450	two fermentation systems	427:450	two fermentation systems (conventional thermal starch liquefaction and native starch hydrolysis)	427:522	Fermentation properties of corn and grain sorghum were compared utilizing two fermentation systems (conventional thermal starch liquefaction and native starch hydrolysis).
27992794	5	36	theme	Dried	905:909	arg1	Grains					911:916	the Distillers Dried Grains	890:916	the Distillers Dried Grains with Solubles	890:930	Lipid analysis of the Distillers Dried Grains with Solubles showed statistically significant differences between corn and sorghum in triacylglycerol, diacylglycerol and free fatty acid levels.
27992794	0	37	theme	lipid	119:123	arg1	profiles					125:132	lipid profiles	119:132	lipid profiles	119:132	A comparison between corn and grain sorghum fermentation rates, Distillers Dried Grains with Solubles composition, and lipid profiles.
27992794	1	38	theme	compositional	181:193	arg1	difference					195:204	the compositional difference	177:204	the compositional difference between grain sorghum and corn impact ethanol	177:250	The aim of this study was to determine if the compositional difference between grain sorghum and corn impact ethanol yields and coproduct value when grain sorghum is incorporated into existing corn ethanol facilities.
27992794	0	39	theme	fermentation	44:55	arg1	rates					57:61	corn and grain sorghum fermentation rates	21:61	corn and grain sorghum fermentation rates	21:61	A comparison between corn and grain sorghum fermentation rates, Distillers Dried Grains with Solubles composition, and lipid profiles.
27992794	1	40	theme	grain	284:288	arg1	sorghum					290:296	grain sorghum	284:296	grain sorghum	284:296	The aim of this study was to determine if the compositional difference between grain sorghum and corn impact ethanol yields and coproduct value when grain sorghum is incorporated into existing corn ethanol facilities.
27992794	5	41	with	Grains	911:916	arg1	Solubles					923:930	Solubles	923:930	Solubles	923:930	Lipid analysis of the Distillers Dried Grains with Solubles showed statistically significant differences between corn and sorghum in triacylglycerol, diacylglycerol and free fatty acid levels.
27992794	2	42	theme	native	498:503	arg1	systems					444:450	two fermentation systems	427:450	two fermentation systems (conventional thermal starch liquefaction and native starch hydrolysis)	427:522	Fermentation properties of corn and grain sorghum were compared utilizing two fermentation systems (conventional thermal starch liquefaction and native starch hydrolysis).
27992794	2	42	theme	native	498:503	arg1	hydrolysis					512:521	native starch hydrolysis	498:521	native starch hydrolysis	498:521	Fermentation properties of corn and grain sorghum were compared utilizing two fermentation systems (conventional thermal starch liquefaction and native starch hydrolysis).
27992794	0	43	theme	sorghum	36:42	arg1	rates					57:61	corn and grain sorghum fermentation rates	21:61	corn and grain sorghum fermentation rates	21:61	A comparison between corn and grain sorghum fermentation rates, Distillers Dried Grains with Solubles composition, and lipid profiles.
27992794	3	44	theme	treated	684:690	arg1	fermentations					692:704	non-protease treated fermentations	671:704	non-protease treated fermentations	671:704	Fermentation results indicated that protease addition influenced the fermentation rate and yield for grain sorghum, improving yields by 1-2% over non-protease treated fermentations.
27992794	0	45	with	profiles	125:132	arg1	composition					102:112	Solubles composition	93:112	Solubles composition	93:112	A comparison between corn and grain sorghum fermentation rates, Distillers Dried Grains with Solubles composition, and lipid profiles.
27992794	5	46	theme	acid	1052:1055	arg1	levels					1057:1062	triacylglycerol, diacylglycerol and free fatty acid levels	1005:1062	triacylglycerol, diacylglycerol and free fatty acid levels	1005:1062	Lipid analysis of the Distillers Dried Grains with Solubles showed statistically significant differences between corn and sorghum in triacylglycerol, diacylglycerol and free fatty acid levels.
27992794	4	47	theme	higher	831:836	arg1	content					846:852	significantly higher protein content	817:852	significantly higher protein content	817:852	Distillers Dried Grains with Solubles produced from sorghum had a statistically significant higher yields and significantly higher protein content relative to corn.
27992794	5	48	theme	diacylglycerol	1022:1035	arg1	levels					1057:1062	triacylglycerol, diacylglycerol and free fatty acid levels	1005:1062	triacylglycerol, diacylglycerol and free fatty acid levels	1005:1062	Lipid analysis of the Distillers Dried Grains with Solubles showed statistically significant differences between corn and sorghum in triacylglycerol, diacylglycerol and free fatty acid levels.
27992794	2	49	theme	starch	505:510	arg1	systems					444:450	two fermentation systems	427:450	two fermentation systems (conventional thermal starch liquefaction and native starch hydrolysis)	427:522	Fermentation properties of corn and grain sorghum were compared utilizing two fermentation systems (conventional thermal starch liquefaction and native starch hydrolysis).
27992794	2	49	theme	starch	505:510	arg1	hydrolysis					512:521	native starch hydrolysis	498:521	native starch hydrolysis	498:521	Fermentation properties of corn and grain sorghum were compared utilizing two fermentation systems (conventional thermal starch liquefaction and native starch hydrolysis).
27992794	2	50	theme	grain	389:393	arg1	sorghum					395:401	grain sorghum	389:401	grain sorghum	389:401	Fermentation properties of corn and grain sorghum were compared utilizing two fermentation systems (conventional thermal starch liquefaction and native starch hydrolysis).
27992794	3	51	theme	grain	626:630	arg1	sorghum					632:638	grain sorghum	626:638	grain sorghum	626:638	Fermentation results indicated that protease addition influenced the fermentation rate and yield for grain sorghum, improving yields by 1-2% over non-protease treated fermentations.
27992794	2	52	theme	Fermentation	353:364	arg1	properties					366:375	Fermentation properties	353:375	Fermentation properties of corn and grain sorghum	353:401	Fermentation properties of corn and grain sorghum were compared utilizing two fermentation systems (conventional thermal starch liquefaction and native starch hydrolysis).
27992794	2	53	theme	corn	380:383	arg1	properties					366:375	Fermentation properties	353:375	Fermentation properties of corn and grain sorghum	353:401	Fermentation properties of corn and grain sorghum were compared utilizing two fermentation systems (conventional thermal starch liquefaction and native starch hydrolysis).
27992794	1	54	theme	grain	214:218	arg1	sorghum					220:226	grain sorghum	214:226	grain sorghum	214:226	The aim of this study was to determine if the compositional difference between grain sorghum and corn impact ethanol yields and coproduct value when grain sorghum is incorporated into existing corn ethanol facilities.
27992794	2	55	theme	conventional	453:464	arg1	liquefaction					481:492	conventional thermal starch liquefaction	453:492	conventional thermal starch liquefaction	453:492	Fermentation properties of corn and grain sorghum were compared utilizing two fermentation systems (conventional thermal starch liquefaction and native starch hydrolysis).
27992794	2	55	theme	conventional	453:464	arg1	systems					444:450	two fermentation systems	427:450	two fermentation systems (conventional thermal starch liquefaction and native starch hydrolysis)	427:522	Fermentation properties of corn and grain sorghum were compared utilizing two fermentation systems (conventional thermal starch liquefaction and native starch hydrolysis).
27992794	4	56	theme	protein	838:844	arg1	content					846:852	significantly higher protein content	817:852	significantly higher protein content	817:852	Distillers Dried Grains with Solubles produced from sorghum had a statistically significant higher yields and significantly higher protein content relative to corn.
27992794	3	57	theme	Fermentation	525:536	arg1	results					538:544	Fermentation results	525:544	Fermentation results	525:544	Fermentation results indicated that protease addition influenced the fermentation rate and yield for grain sorghum, improving yields by 1-2% over non-protease treated fermentations.
25275801	8	0	from	properties	1135:1144	arg1	terms					1094:1098	terms	1094:1098	terms of their impact on the dissolution properties	1094:1144	A comparison of the various matrix materials in terms of their impact on the dissolution properties show a two times faster zero order release from methyl cellulose matrix compared to a polystyrene matrix.
25275801	4	1	theme	drop	568:571	arg1	casting					573:579	drop casting	568:579	drop casting	568:579	The film properties were either varied by the preparation route, i.e., spin coating or drop casting, or via changes in the relative ratio of the ibuprofen and the matrix material.
25275801	4	2	from	material	651:658	arg1	ratio					613:617	the relative ratio	600:617	the relative ratio of the ibuprofen	600:634	The film properties were either varied by the preparation route, i.e., spin coating or drop casting, or via changes in the relative ratio of the ibuprofen and the matrix material.
25275801	6	3	theme	glass	861:865	arg1	surface					867:873	the glass surface	857:873	the glass surface	857:873	The results show that preferred (100) textures can be induced via spin coating with respect to the glass surface, while the drop casting results in a powder-like behavior.
25275801	6	4	theme	preferred	784:792	arg1	textures					800:807	preferred (100) textures	784:807	preferred (100) textures	784:807	The results show that preferred (100) textures can be induced via spin coating with respect to the glass surface, while the drop casting results in a powder-like behavior.
25275801	4	5	theme	ibuprofen	626:634	arg1	ratio					613:617	the relative ratio	600:617	the relative ratio of the ibuprofen	600:634	The film properties were either varied by the preparation route, i.e., spin coating or drop casting, or via changes in the relative ratio of the ibuprofen and the matrix material.
25275801	11	6	theme	similar	1601:1607	arg1	approach					1622:1629	A similar experimental approach	1599:1629	A similar experimental approach using other matrix materials	1599:1658	A similar experimental approach using other matrix materials may therefore allow to find an optimized composite layer useful for a defined application.
25275801	8	7	theme	dissolution	1123:1133	arg1	properties					1135:1144	the dissolution properties	1119:1144	the dissolution properties	1119:1144	A comparison of the various matrix materials in terms of their impact on the dissolution properties show a two times faster zero order release from methyl cellulose matrix compared to a polystyrene matrix.
25275801	10	8	theme	matrix	1544:1549	arg1	material					1551:1558	the selected matrix material	1531:1558	the selected matrix material than that compared to the dissolution	1531:1596	The investigation reveals that the ibuprofen crystallization and film formation is only little effected by the selected matrix material than that compared to the dissolution.
25275801	2	9	theme	material	298:305	arg1	impact					277:282	the impact	273:282	the impact of the matrix material on the film forming properties of ibuprofen-matrix composite films	273:372	Within this model study the impact of the matrix material on the film forming properties of ibuprofen-matrix composite films is investigated.
25275801	1	10	theme	applications	187:198	arg1	applications					187:198	applications	187:198	applications like transdermal, buccal, or sublingual patches	187:246	The preparation of thin composite layers has promising advantages in a variety of applications like transdermal, buccal, or sublingual patches.
25275801	1	10	theme	applications	187:198	arg1	variety					176:182	a variety	174:182	a variety of applications like transdermal, buccal, or sublingual patches	174:246	The preparation of thin composite layers has promising advantages in a variety of applications like transdermal, buccal, or sublingual patches.
25275801	6	11	theme	powder-like	912:922	arg1	behavior					924:931	a powder-like behavior	910:931	a powder-like behavior	910:931	The results show that preferred (100) textures can be induced via spin coating with respect to the glass surface, while the drop casting results in a powder-like behavior.
25275801	8	12	theme	cellulose	1201:1209	arg1	matrix					1211:1216	methyl cellulose matrix	1194:1216	methyl cellulose matrix compared to a polystyrene matrix	1194:1249	A comparison of the various matrix materials in terms of their impact on the dissolution properties show a two times faster zero order release from methyl cellulose matrix compared to a polystyrene matrix.
25275801	1	13	theme	transdermal	205:215	arg1	patches					240:246	transdermal, buccal, or sublingual patches	205:246	transdermal, buccal, or sublingual patches	205:246	The preparation of thin composite layers has promising advantages in a variety of applications like transdermal, buccal, or sublingual patches.
25275801	8	14	theme	two	1153:1155	arg1	times					1157:1161	times	1157:1161	times	1157:1161	A comparison of the various matrix materials in terms of their impact on the dissolution properties show a two times faster zero order release from methyl cellulose matrix compared to a polystyrene matrix.
25275801	1	15	theme	buccal	218:223	arg1	patches					240:246	transdermal, buccal, or sublingual patches	205:246	transdermal, buccal, or sublingual patches	205:246	The preparation of thin composite layers has promising advantages in a variety of applications like transdermal, buccal, or sublingual patches.
25275801	7	16	theme	ibuprofen	993:1001	arg1	amount					1003:1008	the ibuprofen amount	989:1008	the ibuprofen amount rather than the preparation method	989:1043	The morphologies of the films are strongly impacted by the ibuprofen amount rather than the preparation method.
25275801	1	17	theme	thin	124:127	arg1	layers					139:144	thin composite layers	124:144	thin composite layers	124:144	The preparation of thin composite layers has promising advantages in a variety of applications like transdermal, buccal, or sublingual patches.
25275801	11	18	theme	composite	1701:1709	arg1	layer					1711:1715	an optimized composite layer	1688:1715	an optimized composite layer useful for a defined application	1688:1748	A similar experimental approach using other matrix materials may therefore allow to find an optimized composite layer useful for a defined application.
25275801	1	19	theme	layers	139:144	arg1	preparation					109:119	The preparation	105:119	The preparation of thin composite layers	105:144	The preparation of thin composite layers has promising advantages in a variety of applications like transdermal, buccal, or sublingual patches.
25275801	5	20	theme	force	732:736	arg1	microscope					738:747	atomic force microscope	725:747	atomic force microscope	725:747	The resulting films were investigated via X-ray diffraction and atomic force microscope experiments.
25275801	1	21	theme	promising	150:158	arg1	advantages					160:169	promising advantages	150:169	promising advantages	150:169	The preparation of thin composite layers has promising advantages in a variety of applications like transdermal, buccal, or sublingual patches.
25275801	11	22	theme	useful	1717:1722	arg1	layer					1711:1715	an optimized composite layer	1688:1715	an optimized composite layer useful for a defined application	1688:1748	A similar experimental approach using other matrix materials may therefore allow to find an optimized composite layer useful for a defined application.
25275801	5	23	theme	X-ray	703:707	arg1	diffraction					709:719	X-ray diffraction	703:719	X-ray diffraction	703:719	The resulting films were investigated via X-ray diffraction and atomic force microscope experiments.
25275801	4	24	dep	coating	557:563	arg1	i.e.					546:549	i.e.	546:549	i.e.	546:549	The film properties were either varied by the preparation route, i.e., spin coating or drop casting, or via changes in the relative ratio of the ibuprofen and the matrix material.
25275801	8	25	from	comparison	1048:1057	arg1	terms					1094:1098	terms	1094:1098	terms of their impact on the dissolution properties	1094:1144	A comparison of the various matrix materials in terms of their impact on the dissolution properties show a two times faster zero order release from methyl cellulose matrix compared to a polystyrene matrix.
25275801	9	26	theme	slowest	1256:1262	arg1	rate					1264:1267	The slowest rate	1252:1267	The slowest rate	1252:1267	The slowest rate was observed within the hydroxyl ethyl cellulose as the active pharmaceutical ingredients (APIs) release is limited by diffusion through a swollen matrix.
25275801	4	27	theme	film	485:488	arg1	either					506:511	either	506:511	either	506:511	The film properties were either varied by the preparation route, i.e., spin coating or drop casting, or via changes in the relative ratio of the ibuprofen and the matrix material.
25275801	4	27	theme	film	485:488	arg1	properties					490:499	The film properties	481:499	The film properties	481:499	The film properties were either varied by the preparation route, i.e., spin coating or drop casting, or via changes in the relative ratio of the ibuprofen and the matrix material.
25275801	4	27	theme	film	485:488	arg1	varied					513:518	varied	513:518	varied	513:518	The film properties were either varied by the preparation route, i.e., spin coating or drop casting, or via changes in the relative ratio of the ibuprofen and the matrix material.
25275801	3	28	theme	methyl	424:429	arg1	materials					401:409	As matrix materials	391:409	As matrix materials polystyrene, methyl cellulose, or hydroxyl-ethyl cellulose	391:468	As matrix materials polystyrene, methyl cellulose, or hydroxyl-ethyl cellulose were used.
25275801	3	28	theme	methyl	424:429	arg1	cellulose					431:439	methyl cellulose	424:439	methyl cellulose	424:439	As matrix materials polystyrene, methyl cellulose, or hydroxyl-ethyl cellulose were used.
25275801	0	29	theme	Crystallographic	0:15	arg1	textures					17:24	Crystallographic textures	0:24	Crystallographic textures	0:24	Crystallographic textures and morphologies of solution cast Ibuprofen composite films at solid surfaces.
25275801	8	30	dep	release	1181:1187	arg1	times					1157:1161	times	1157:1161	times	1157:1161	A comparison of the various matrix materials in terms of their impact on the dissolution properties show a two times faster zero order release from methyl cellulose matrix compared to a polystyrene matrix.
25275801	3	31	used	used	475:478	arg2	materials					401:409	As matrix materials	391:409	As matrix materials polystyrene, methyl cellulose, or hydroxyl-ethyl cellulose	391:468	As matrix materials polystyrene, methyl cellulose, or hydroxyl-ethyl cellulose were used.
25275801	3	31	used	used	475:478	arg2	polystyrene					411:421	polystyrene	411:421	polystyrene	411:421	As matrix materials polystyrene, methyl cellulose, or hydroxyl-ethyl cellulose were used.
25275801	3	31	used	used	475:478	arg2	cellulose					460:468	hydroxyl-ethyl cellulose	445:468	hydroxyl-ethyl cellulose	445:468	As matrix materials polystyrene, methyl cellulose, or hydroxyl-ethyl cellulose were used.
25275801	3	31	used	used	475:478	arg2	cellulose					431:439	methyl cellulose	424:439	methyl cellulose	424:439	As matrix materials polystyrene, methyl cellulose, or hydroxyl-ethyl cellulose were used.
25275801	8	32	from	terms	1094:1098	arg1	properties					1135:1144	the dissolution properties	1119:1144	the dissolution properties	1119:1144	A comparison of the various matrix materials in terms of their impact on the dissolution properties show a two times faster zero order release from methyl cellulose matrix compared to a polystyrene matrix.
25275801	8	32	from	terms	1094:1098	arg1	comparison					1048:1057	A comparison	1046:1057	A comparison of the various matrix materials in terms of their impact on the dissolution properties	1046:1144	A comparison of the various matrix materials in terms of their impact on the dissolution properties show a two times faster zero order release from methyl cellulose matrix compared to a polystyrene matrix.
25275801	4	33	theme	spin	552:555	arg1	coating					557:563	spin coating	552:563	spin coating	552:563	The film properties were either varied by the preparation route, i.e., spin coating or drop casting, or via changes in the relative ratio of the ibuprofen and the matrix material.
25275801	3	34	theme	hydroxyl-ethyl	445:458	arg1	cellulose					460:468	hydroxyl-ethyl cellulose	445:468	hydroxyl-ethyl cellulose	445:468	As matrix materials polystyrene, methyl cellulose, or hydroxyl-ethyl cellulose were used.
25275801	3	34	theme	hydroxyl-ethyl	445:458	arg1	materials					401:409	As matrix materials	391:409	As matrix materials polystyrene, methyl cellulose, or hydroxyl-ethyl cellulose	391:468	As matrix materials polystyrene, methyl cellulose, or hydroxyl-ethyl cellulose were used.
25275801	9	35	theme	hydroxyl	1293:1300	arg1	cellulose					1308:1316	the hydroxyl ethyl cellulose	1289:1316	the hydroxyl ethyl cellulose as the active pharmaceutical ingredients (APIs) release is limited by diffusion through a swollen matrix	1289:1421	The slowest rate was observed within the hydroxyl ethyl cellulose as the active pharmaceutical ingredients (APIs) release is limited by diffusion through a swollen matrix.
25275801	8	36	from	materials	1081:1089	arg1	terms					1094:1098	terms	1094:1098	terms of their impact on the dissolution properties	1094:1144	A comparison of the various matrix materials in terms of their impact on the dissolution properties show a two times faster zero order release from methyl cellulose matrix compared to a polystyrene matrix.
25275801	3	37	theme	matrix	394:399	arg1	materials					401:409	As matrix materials	391:409	As matrix materials polystyrene, methyl cellulose, or hydroxyl-ethyl cellulose	391:468	As matrix materials polystyrene, methyl cellulose, or hydroxyl-ethyl cellulose were used.
25275801	3	37	theme	matrix	394:399	arg1	polystyrene					411:421	polystyrene	411:421	polystyrene	411:421	As matrix materials polystyrene, methyl cellulose, or hydroxyl-ethyl cellulose were used.
25275801	3	37	theme	matrix	394:399	arg1	cellulose					431:439	methyl cellulose	424:439	methyl cellulose	424:439	As matrix materials polystyrene, methyl cellulose, or hydroxyl-ethyl cellulose were used.
25275801	3	37	theme	matrix	394:399	arg1	cellulose					460:468	hydroxyl-ethyl cellulose	445:468	hydroxyl-ethyl cellulose	445:468	As matrix materials polystyrene, methyl cellulose, or hydroxyl-ethyl cellulose were used.
25275801	11	38	theme	matrix	1643:1648	arg1	materials					1650:1658	other matrix materials	1637:1658	other matrix materials	1637:1658	A similar experimental approach using other matrix materials may therefore allow to find an optimized composite layer useful for a defined application.
25275801	4	39	dep	either	506:511	arg1	either					506:511	either	506:511	either	506:511	The film properties were either varied by the preparation route, i.e., spin coating or drop casting, or via changes in the relative ratio of the ibuprofen and the matrix material.
25275801	4	39	dep	either	506:511	arg1	properties					490:499	The film properties	481:499	The film properties	481:499	The film properties were either varied by the preparation route, i.e., spin coating or drop casting, or via changes in the relative ratio of the ibuprofen and the matrix material.
25275801	4	39	dep	either	506:511	arg1	varied					513:518	varied	513:518	varied	513:518	The film properties were either varied by the preparation route, i.e., spin coating or drop casting, or via changes in the relative ratio of the ibuprofen and the matrix material.
25275801	2	40	theme	film	314:317	arg1	properties					327:336	the film forming properties	310:336	the film forming properties of ibuprofen-matrix composite films	310:372	Within this model study the impact of the matrix material on the film forming properties of ibuprofen-matrix composite films is investigated.
25275801	2	41	theme	films	368:372	arg1	properties					327:336	the film forming properties	310:336	the film forming properties of ibuprofen-matrix composite films	310:372	Within this model study the impact of the matrix material on the film forming properties of ibuprofen-matrix composite films is investigated.
25275801	9	42	theme	pharmaceutical	1332:1345	arg1	APIs					1360:1363	APIs	1360:1363	APIs	1360:1363	The slowest rate was observed within the hydroxyl ethyl cellulose as the active pharmaceutical ingredients (APIs) release is limited by diffusion through a swollen matrix.
25275801	9	42	theme	pharmaceutical	1332:1345	arg1	ingredients					1347:1357	the active pharmaceutical ingredients	1321:1357	the active pharmaceutical ingredients (APIs) release	1321:1372	The slowest rate was observed within the hydroxyl ethyl cellulose as the active pharmaceutical ingredients (APIs) release is limited by diffusion through a swollen matrix.
25275801	8	43	theme	matrix	1074:1079	arg1	materials					1081:1089	the various matrix materials	1062:1089	the various matrix materials in terms of their impact on the dissolution properties	1062:1144	A comparison of the various matrix materials in terms of their impact on the dissolution properties show a two times faster zero order release from methyl cellulose matrix compared to a polystyrene matrix.
25275801	4	44	theme	relative	604:611	arg1	ratio					613:617	the relative ratio	600:617	the relative ratio of the ibuprofen	600:634	The film properties were either varied by the preparation route, i.e., spin coating or drop casting, or via changes in the relative ratio of the ibuprofen and the matrix material.
25275801	10	45	theme	film	1489:1492	arg1	formation					1494:1502	the ibuprofen crystallization and film formation	1455:1502	formation	1494:1502	The investigation reveals that the ibuprofen crystallization and film formation is only little effected by the selected matrix material than that compared to the dissolution.
25275801	5	46	theme	microscope	738:747	arg1	experiments					749:759	X-ray diffraction and atomic force microscope experiments	703:759	X-ray diffraction and atomic force microscope experiments	703:759	The resulting films were investigated via X-ray diffraction and atomic force microscope experiments.
25275801	11	47	theme	experimental	1609:1620	arg1	approach					1622:1629	A similar experimental approach	1599:1629	A similar experimental approach using other matrix materials	1599:1658	A similar experimental approach using other matrix materials may therefore allow to find an optimized composite layer useful for a defined application.
25275801	2	48	theme	ibuprofen-matrix	341:356	arg1	films					368:372	ibuprofen-matrix composite films	341:372	ibuprofen-matrix composite films	341:372	Within this model study the impact of the matrix material on the film forming properties of ibuprofen-matrix composite films is investigated.
25275801	8	49	theme	impact	1109:1114	arg1	terms					1094:1098	terms	1094:1098	terms of their impact on the dissolution properties	1094:1144	A comparison of the various matrix materials in terms of their impact on the dissolution properties show a two times faster zero order release from methyl cellulose matrix compared to a polystyrene matrix.
25275801	1	50	contain	has	146:148	arg2	advantages					160:169	promising advantages	150:169	promising advantages	150:169	The preparation of thin composite layers has promising advantages in a variety of applications like transdermal, buccal, or sublingual patches.
25275801	1	50	contain	has	146:148	arg1	preparation					109:119	The preparation	105:119	The preparation of thin composite layers	105:144	The preparation of thin composite layers has promising advantages in a variety of applications like transdermal, buccal, or sublingual patches.
25275801	7	51	theme	preparation	1026:1036	arg1	method					1038:1043	the preparation method	1022:1043	the ibuprofen amount rather than the preparation method	989:1043	The morphologies of the films are strongly impacted by the ibuprofen amount rather than the preparation method.
25275801	10	52	theme	selected	1535:1542	arg1	material					1551:1558	the selected matrix material	1531:1558	the selected matrix material than that compared to the dissolution	1531:1596	The investigation reveals that the ibuprofen crystallization and film formation is only little effected by the selected matrix material than that compared to the dissolution.
25275801	5	53	theme	resulting	665:673	arg1	films					675:679	The resulting films	661:679	The resulting films	661:679	The resulting films were investigated via X-ray diffraction and atomic force microscope experiments.
25275801	2	54	theme	matrix	291:296	arg1	material					298:305	the matrix material	287:305	the matrix material	287:305	Within this model study the impact of the matrix material on the film forming properties of ibuprofen-matrix composite films is investigated.
25275801	8	55	theme	methyl	1194:1199	arg1	matrix					1211:1216	methyl cellulose matrix	1194:1216	methyl cellulose matrix compared to a polystyrene matrix	1194:1249	A comparison of the various matrix materials in terms of their impact on the dissolution properties show a two times faster zero order release from methyl cellulose matrix compared to a polystyrene matrix.
25275801	9	56	theme	swollen	1408:1414	arg1	matrix					1416:1421	a swollen matrix	1406:1421	a swollen matrix	1406:1421	The slowest rate was observed within the hydroxyl ethyl cellulose as the active pharmaceutical ingredients (APIs) release is limited by diffusion through a swollen matrix.
25275801	9	57	located	observed	1273:1280	arg2	rate					1264:1267	The slowest rate	1252:1267	The slowest rate	1252:1267	The slowest rate was observed within the hydroxyl ethyl cellulose as the active pharmaceutical ingredients (APIs) release is limited by diffusion through a swollen matrix.
25275801	9	57	located	observed	1273:1280	arg1	cellulose					1308:1316	the hydroxyl ethyl cellulose	1289:1316	the hydroxyl ethyl cellulose as the active pharmaceutical ingredients (APIs) release is limited by diffusion through a swollen matrix	1289:1421	The slowest rate was observed within the hydroxyl ethyl cellulose as the active pharmaceutical ingredients (APIs) release is limited by diffusion through a swollen matrix.
25275801	6	58	theme	drop	886:889	arg1	casting					891:897	the drop casting	882:897	the drop casting	882:897	The results show that preferred (100) textures can be induced via spin coating with respect to the glass surface, while the drop casting results in a powder-like behavior.
25275801	2	59	theme	model	261:265	arg1	study					267:271	this model study	256:271	this model study	256:271	Within this model study the impact of the matrix material on the film forming properties of ibuprofen-matrix composite films is investigated.
25275801	7	60	theme	films	958:962	arg1	morphologies					938:949	The morphologies	934:949	The morphologies of the films	934:962	The morphologies of the films are strongly impacted by the ibuprofen amount rather than the preparation method.
25275801	1	61	theme	composite	129:137	arg1	layers					139:144	thin composite layers	124:144	thin composite layers	124:144	The preparation of thin composite layers has promising advantages in a variety of applications like transdermal, buccal, or sublingual patches.
25275801	8	62	theme	polystyrene	1232:1242	arg1	matrix					1244:1249	a polystyrene matrix	1230:1249	a polystyrene matrix	1230:1249	A comparison of the various matrix materials in terms of their impact on the dissolution properties show a two times faster zero order release from methyl cellulose matrix compared to a polystyrene matrix.
25275801	5	63	theme	atomic	725:730	arg1	microscope					738:747	atomic force microscope	725:747	atomic force microscope	725:747	The resulting films were investigated via X-ray diffraction and atomic force microscope experiments.
25275801	8	64	from	matrix	1211:1216	arg1	release					1181:1187	a two times faster zero order release	1151:1187	a two times faster zero order release from methyl cellulose matrix compared to a polystyrene matrix	1151:1249	A comparison of the various matrix materials in terms of their impact on the dissolution properties show a two times faster zero order release from methyl cellulose matrix compared to a polystyrene matrix.
25275801	2	65	from	impact	277:282	arg1	properties					327:336	the film forming properties	310:336	the film forming properties of ibuprofen-matrix composite films	310:372	Within this model study the impact of the matrix material on the film forming properties of ibuprofen-matrix composite films is investigated.
25275801	4	66	theme	matrix	644:649	arg1	material					651:658	the matrix material	640:658	the matrix material	640:658	The film properties were either varied by the preparation route, i.e., spin coating or drop casting, or via changes in the relative ratio of the ibuprofen and the matrix material.
25275801	11	67	theme	optimized	1691:1699	arg1	layer					1711:1715	an optimized composite layer	1688:1715	an optimized composite layer useful for a defined application	1688:1748	A similar experimental approach using other matrix materials may therefore allow to find an optimized composite layer useful for a defined application.
25275801	0	68	theme	solid	89:93	arg1	surfaces					95:102	solid surfaces	89:102	solid surfaces	89:102	Crystallographic textures and morphologies of solution cast Ibuprofen composite films at solid surfaces.
25275801	11	69	theme	defined	1730:1736	arg1	application					1738:1748	a defined application	1728:1748	a defined application	1728:1748	A similar experimental approach using other matrix materials may therefore allow to find an optimized composite layer useful for a defined application.
25275801	9	70	dep	cellulose	1308:1316	arg1	limited					1377:1383	limited	1377:1383	is limited by diffusion through a swollen matrix	1374:1421	The slowest rate was observed within the hydroxyl ethyl cellulose as the active pharmaceutical ingredients (APIs) release is limited by diffusion through a swollen matrix.
25275801	5	71	theme	diffraction	709:719	arg1	experiments					749:759	X-ray diffraction and atomic force microscope experiments	703:759	X-ray diffraction and atomic force microscope experiments	703:759	The resulting films were investigated via X-ray diffraction and atomic force microscope experiments.
25275801	3	72	dep	materials	401:409	arg1	cellulose					460:468	hydroxyl-ethyl cellulose	445:468	hydroxyl-ethyl cellulose	445:468	As matrix materials polystyrene, methyl cellulose, or hydroxyl-ethyl cellulose were used.
25275801	3	72	dep	materials	401:409	arg1	polystyrene					411:421	polystyrene	411:421	polystyrene	411:421	As matrix materials polystyrene, methyl cellulose, or hydroxyl-ethyl cellulose were used.
25275801	3	72	dep	materials	401:409	arg1	cellulose					431:439	methyl cellulose	424:439	methyl cellulose	424:439	As matrix materials polystyrene, methyl cellulose, or hydroxyl-ethyl cellulose were used.
25275801	3	72	dep	materials	401:409	arg1	materials					401:409	As matrix materials	391:409	As matrix materials polystyrene, methyl cellulose, or hydroxyl-ethyl cellulose	391:468	As matrix materials polystyrene, methyl cellulose, or hydroxyl-ethyl cellulose were used.
25275801	3	73	theme	As	391:392	arg1	materials					401:409	As matrix materials	391:409	As matrix materials polystyrene, methyl cellulose, or hydroxyl-ethyl cellulose	391:468	As matrix materials polystyrene, methyl cellulose, or hydroxyl-ethyl cellulose were used.
25275801	3	73	theme	As	391:392	arg1	polystyrene					411:421	polystyrene	411:421	polystyrene	411:421	As matrix materials polystyrene, methyl cellulose, or hydroxyl-ethyl cellulose were used.
25275801	3	73	theme	As	391:392	arg1	cellulose					431:439	methyl cellulose	424:439	methyl cellulose	424:439	As matrix materials polystyrene, methyl cellulose, or hydroxyl-ethyl cellulose were used.
25275801	3	73	theme	As	391:392	arg1	cellulose					460:468	hydroxyl-ethyl cellulose	445:468	hydroxyl-ethyl cellulose	445:468	As matrix materials polystyrene, methyl cellulose, or hydroxyl-ethyl cellulose were used.
25275801	8	74	dep	times	1157:1161	arg1	order					1175:1179	zero order	1170:1179	zero order	1170:1179	A comparison of the various matrix materials in terms of their impact on the dissolution properties show a two times faster zero order release from methyl cellulose matrix compared to a polystyrene matrix.
25275801	8	74	dep	times	1157:1161	arg1	faster					1163:1168	faster	1163:1168	faster	1163:1168	A comparison of the various matrix materials in terms of their impact on the dissolution properties show a two times faster zero order release from methyl cellulose matrix compared to a polystyrene matrix.
25275801	0	75	theme	solution	46:53	arg1	morphologies					30:41	morphologies	30:41	morphologies	30:41	Crystallographic textures and morphologies of solution cast Ibuprofen composite films at solid surfaces.
25275801	0	75	theme	solution	46:53	arg1	textures					17:24	Crystallographic textures	0:24	Crystallographic textures	0:24	Crystallographic textures and morphologies of solution cast Ibuprofen composite films at solid surfaces.
25275801	6	76	theme	spin	828:831	arg1	coating					833:839	spin coating	828:839	spin coating with respect to the glass surface	828:873	The results show that preferred (100) textures can be induced via spin coating with respect to the glass surface, while the drop casting results in a powder-like behavior.
25275801	4	77	theme	preparation	527:537	arg1	route					539:543	the preparation route	523:543	the preparation route	523:543	The film properties were either varied by the preparation route, i.e., spin coating or drop casting, or via changes in the relative ratio of the ibuprofen and the matrix material.
25275801	11	78	theme	other	1637:1641	arg1	materials					1650:1658	other matrix materials	1637:1658	other matrix materials	1637:1658	A similar experimental approach using other matrix materials may therefore allow to find an optimized composite layer useful for a defined application.
25275801	1	79	theme	sublingual	229:238	arg1	patches					240:246	transdermal, buccal, or sublingual patches	205:246	transdermal, buccal, or sublingual patches	205:246	The preparation of thin composite layers has promising advantages in a variety of applications like transdermal, buccal, or sublingual patches.
25275801	0	80	theme	Ibuprofen	60:68	arg1	films					80:84	Ibuprofen composite films	60:84	Ibuprofen composite films	60:84	Crystallographic textures and morphologies of solution cast Ibuprofen composite films at solid surfaces.
25275801	9	81	theme	ethyl	1302:1306	arg1	cellulose					1308:1316	the hydroxyl ethyl cellulose	1289:1316	the hydroxyl ethyl cellulose as the active pharmaceutical ingredients (APIs) release is limited by diffusion through a swollen matrix	1289:1421	The slowest rate was observed within the hydroxyl ethyl cellulose as the active pharmaceutical ingredients (APIs) release is limited by diffusion through a swollen matrix.
25275801	0	82	theme	composite	70:78	arg1	films					80:84	Ibuprofen composite films	60:84	Ibuprofen composite films	60:84	Crystallographic textures and morphologies of solution cast Ibuprofen composite films at solid surfaces.
25275801	10	83	theme	ibuprofen	1459:1467	arg1	little					1512:1517	little	1512:1517	little	1512:1517	The investigation reveals that the ibuprofen crystallization and film formation is only little effected by the selected matrix material than that compared to the dissolution.
25275801	10	83	theme	ibuprofen	1459:1467	arg1	crystallization					1469:1483	the ibuprofen crystallization and film formation	1455:1502	crystallization	1469:1483	The investigation reveals that the ibuprofen crystallization and film formation is only little effected by the selected matrix material than that compared to the dissolution.
25275801	2	84	theme	forming	319:325	arg1	properties					327:336	the film forming properties	310:336	the film forming properties of ibuprofen-matrix composite films	310:372	Within this model study the impact of the matrix material on the film forming properties of ibuprofen-matrix composite films is investigated.
25275801	8	85	theme	various	1066:1072	arg1	materials					1081:1089	the various matrix materials	1062:1089	the various matrix materials in terms of their impact on the dissolution properties	1062:1144	A comparison of the various matrix materials in terms of their impact on the dissolution properties show a two times faster zero order release from methyl cellulose matrix compared to a polystyrene matrix.
25275801	8	86	from	impact	1109:1114	arg1	properties					1135:1144	the dissolution properties	1119:1144	the dissolution properties	1119:1144	A comparison of the various matrix materials in terms of their impact on the dissolution properties show a two times faster zero order release from methyl cellulose matrix compared to a polystyrene matrix.
25275801	4	87	from	changes	589:595	arg1	ratio					613:617	the relative ratio	600:617	the relative ratio of the ibuprofen	600:634	The film properties were either varied by the preparation route, i.e., spin coating or drop casting, or via changes in the relative ratio of the ibuprofen and the matrix material.
25275801	9	88	theme	active	1325:1330	arg1	APIs					1360:1363	APIs	1360:1363	APIs	1360:1363	The slowest rate was observed within the hydroxyl ethyl cellulose as the active pharmaceutical ingredients (APIs) release is limited by diffusion through a swollen matrix.
25275801	9	88	theme	active	1325:1330	arg1	ingredients					1347:1357	the active pharmaceutical ingredients	1321:1357	the active pharmaceutical ingredients (APIs) release	1321:1372	The slowest rate was observed within the hydroxyl ethyl cellulose as the active pharmaceutical ingredients (APIs) release is limited by diffusion through a swollen matrix.
25275801	8	89	theme	materials	1081:1089	arg1	comparison					1048:1057	A comparison	1046:1057	A comparison of the various matrix materials in terms of their impact on the dissolution properties	1046:1144	A comparison of the various matrix materials in terms of their impact on the dissolution properties show a two times faster zero order release from methyl cellulose matrix compared to a polystyrene matrix.
25275801	2	90	theme	composite	358:366	arg1	films					368:372	ibuprofen-matrix composite films	341:372	ibuprofen-matrix composite films	341:372	Within this model study the impact of the matrix material on the film forming properties of ibuprofen-matrix composite films is investigated.
25275801	9	91	theme	ingredients	1347:1357	arg1	release					1366:1372	the active pharmaceutical ingredients (APIs) release	1321:1372	the active pharmaceutical ingredients (APIs) release	1321:1372	The slowest rate was observed within the hydroxyl ethyl cellulose as the active pharmaceutical ingredients (APIs) release is limited by diffusion through a swollen matrix.
28938140	5	0	theme	equilibrium	690:700	arg1	isotherms					702:710	sorption equilibrium isotherms	681:710	sorption equilibrium isotherms	681:710	Kinetic data and sorption equilibrium isotherms were also conducted in batch process.
28938140	6	1	theme	Cu	806:807	arg1	sorption					794:801	sorption	794:801	sorption of Cu (II) and U	794:818	The sorption kinetic analysis revealed that sorption of Cu (II) and U (VI) followed the pseudo-second-order model well and exhibited 3-stage intraparticle diffusion model during the whole sorption process.
28938140	7	2	theme	hydrogel	1034:1041	arg1	beads					1043:1047	the CCM composite hydrogel beads	1016:1047	the CCM composite hydrogel beads	1016:1047	Equilibrium data were best described by Langmuir model, and the CCM composite hydrogel beads showed the estimated maximum adsorption capacity 143.276mg/g and 392.692mg/g for Cu (II) and U (VI), respectively.
28938140	7	3	theme	Equilibrium	956:966	arg1	data					968:971	Equilibrium data	956:971	Equilibrium data	956:971	Equilibrium data were best described by Langmuir model, and the CCM composite hydrogel beads showed the estimated maximum adsorption capacity 143.276mg/g and 392.692mg/g for Cu (II) and U (VI), respectively.
28938140	6	4	theme	kinetic	763:769	arg1	analysis					771:778	The sorption kinetic analysis	750:778	The sorption kinetic analysis	750:778	The sorption kinetic analysis revealed that sorption of Cu (II) and U (VI) followed the pseudo-second-order model well and exhibited 3-stage intraparticle diffusion model during the whole sorption process.
28938140	1	5	theme	hydrogel	345:352	arg1	beads					354:358	calcium alginate coated chitosan hydrochloride (CCM) hydrogel beads	292:358	calcium alginate coated chitosan hydrochloride (CCM) hydrogel beads	292:358	The aim of this work was to study the removal of Cu (II) and U (VI) ions from aqueous solutions by encapsulating magnetic Fe3O4 nanoparticles into calcium alginate coated chitosan hydrochloride (CCM) hydrogel beads.
28938140	4	6	theme	ions	602:605	arg1	removal					567:573	removal	567:573	removal of both copper and uranium ions under various experimental conditions	567:643	Adsorption properties for removal of both copper and uranium ions under various experimental conditions were investigated.
28938140	1	7	theme	Cu	194:195	arg1	removal					183:189	the removal	179:189	the removal of Cu (II) and U (VI) ions from aqueous solutions	179:239	The aim of this work was to study the removal of Cu (II) and U (VI) ions from aqueous solutions by encapsulating magnetic Fe3O4 nanoparticles into calcium alginate coated chitosan hydrochloride (CCM) hydrogel beads.
28938140	0	8	theme	Cu	102:103	arg1	removal					91:97	removal	91:97	removal of Cu (II) and U (VI) from aqueous solutions	91:142	Encapsulating Fe3O4 into calcium alginate coated chitosan hydrochloride hydrogel beads for removal of Cu (II) and U (VI) from aqueous solutions.
28938140	6	9	theme	U	818:818	arg1	sorption					794:801	sorption	794:801	sorption of Cu (II) and U	794:818	The sorption kinetic analysis revealed that sorption of Cu (II) and U (VI) followed the pseudo-second-order model well and exhibited 3-stage intraparticle diffusion model during the whole sorption process.
28938140	7	10	theme	CCM	1020:1022	arg1	beads					1043:1047	the CCM composite hydrogel beads	1016:1047	the CCM composite hydrogel beads	1016:1047	Equilibrium data were best described by Langmuir model, and the CCM composite hydrogel beads showed the estimated maximum adsorption capacity 143.276mg/g and 392.692mg/g for Cu (II) and U (VI), respectively.
28938140	8	11	theme	significant	1242:1252	arg1	changes					1254:1260	significant changes	1242:1260	significant changes in the adsorption capacity and structural stability	1242:1312	The CCM adsorbent exhibited excellent reusability for five cycles use without significant changes in the adsorption capacity and structural stability.
28938140	9	12	from	ions	1414:1417	arg1	wastewater					1422:1431	wastewater	1422:1431	wastewater	1422:1431	The results demonstrated that CCM can be an effective and promising sorbent for Cu (II) and U (VI) ions in wastewater.
28938140	6	13	theme	diffusion	905:913	arg1	model					915:919	3-stage intraparticle diffusion model	883:919	3-stage intraparticle diffusion model	883:919	The sorption kinetic analysis revealed that sorption of Cu (II) and U (VI) followed the pseudo-second-order model well and exhibited 3-stage intraparticle diffusion model during the whole sorption process.
28938140	1	14	theme	magnetic	258:265	arg1	nanoparticles					273:285	magnetic Fe3O4 nanoparticles	258:285	magnetic Fe3O4 nanoparticles	258:285	The aim of this work was to study the removal of Cu (II) and U (VI) ions from aqueous solutions by encapsulating magnetic Fe3O4 nanoparticles into calcium alginate coated chitosan hydrochloride (CCM) hydrogel beads.
28938140	7	15	theme	composite	1024:1032	arg1	beads					1043:1047	the CCM composite hydrogel beads	1016:1047	the CCM composite hydrogel beads	1016:1047	Equilibrium data were best described by Langmuir model, and the CCM composite hydrogel beads showed the estimated maximum adsorption capacity 143.276mg/g and 392.692mg/g for Cu (II) and U (VI), respectively.
28938140	8	16	theme	adsorbent	1172:1180	arg1	CCM					1168:1170	The CCM adsorbent	1164:1180	The CCM adsorbent	1164:1180	The CCM adsorbent exhibited excellent reusability for five cycles use without significant changes in the adsorption capacity and structural stability.
28938140	6	17	theme	intraparticle	891:903	arg1	model					915:919	3-stage intraparticle diffusion model	883:919	3-stage intraparticle diffusion model	883:919	The sorption kinetic analysis revealed that sorption of Cu (II) and U (VI) followed the pseudo-second-order model well and exhibited 3-stage intraparticle diffusion model during the whole sorption process.
28938140	5	18	theme	Kinetic	664:670	arg1	data					672:675	Kinetic data	664:675	Kinetic data	664:675	Kinetic data and sorption equilibrium isotherms were also conducted in batch process.
28938140	1	19	theme	Fe3O4	267:271	arg1	nanoparticles					273:285	magnetic Fe3O4 nanoparticles	258:285	magnetic Fe3O4 nanoparticles	258:285	The aim of this work was to study the removal of Cu (II) and U (VI) ions from aqueous solutions by encapsulating magnetic Fe3O4 nanoparticles into calcium alginate coated chitosan hydrochloride (CCM) hydrogel beads.
28938140	5	20	theme	batch	735:739	arg1	process					741:747	batch process	735:747	batch process	735:747	Kinetic data and sorption equilibrium isotherms were also conducted in batch process.
28938140	9	21	dep	Cu	1395:1396	arg1	ions					1414:1417	ions	1414:1417	(VI) ions in wastewater	1409:1431	The results demonstrated that CCM can be an effective and promising sorbent for Cu (II) and U (VI) ions in wastewater.
28938140	1	22	from	solutions	231:239	arg1	removal					183:189	the removal	179:189	the removal of Cu (II) and U (VI) ions from aqueous solutions	179:239	The aim of this work was to study the removal of Cu (II) and U (VI) ions from aqueous solutions by encapsulating magnetic Fe3O4 nanoparticles into calcium alginate coated chitosan hydrochloride (CCM) hydrogel beads.
28938140	7	23	theme	estimated	1060:1068	arg1	capacity					1089:1096	the estimated maximum adsorption capacity 143.276mg/g and 392.692mg/g	1056:1124	capacity	1089:1096	Equilibrium data were best described by Langmuir model, and the CCM composite hydrogel beads showed the estimated maximum adsorption capacity 143.276mg/g and 392.692mg/g for Cu (II) and U (VI), respectively.
28938140	2	24	theme	ATR-FTIR	361:368	arg1	data					387:390	ATR-FTIR and XRD analysis data	361:390	ATR-FTIR and XRD analysis data	361:390	ATR-FTIR and XRD analysis data indicated that the CCM composites were successfully prepared.
28938140	2	25	theme	analysis	378:385	arg1	data					387:390	ATR-FTIR and XRD analysis data	361:390	ATR-FTIR and XRD analysis data	361:390	ATR-FTIR and XRD analysis data indicated that the CCM composites were successfully prepared.
28938140	8	26	theme	structural	1293:1302	arg1	stability					1304:1312	structural stability	1293:1312	structural stability	1293:1312	The CCM adsorbent exhibited excellent reusability for five cycles use without significant changes in the adsorption capacity and structural stability.
28938140	8	27	theme	adsorption	1269:1278	arg1	capacity					1280:1287	the adsorption capacity	1265:1287	the adsorption capacity	1265:1287	The CCM adsorbent exhibited excellent reusability for five cycles use without significant changes in the adsorption capacity and structural stability.
28938140	4	28	theme	copper	583:588	arg1	removal					567:573	removal	567:573	removal of both copper and uranium ions under various experimental conditions	567:643	Adsorption properties for removal of both copper and uranium ions under various experimental conditions were investigated.
28938140	6	29	theme	3-stage	883:889	arg1	model					915:919	3-stage intraparticle diffusion model	883:919	3-stage intraparticle diffusion model	883:919	The sorption kinetic analysis revealed that sorption of Cu (II) and U (VI) followed the pseudo-second-order model well and exhibited 3-stage intraparticle diffusion model during the whole sorption process.
28938140	7	30	dep	U	1142:1142	arg1	VI					1145:1146	VI	1145:1146	VI	1145:1146	Equilibrium data were best described by Langmuir model, and the CCM composite hydrogel beads showed the estimated maximum adsorption capacity 143.276mg/g and 392.692mg/g for Cu (II) and U (VI), respectively.
28938140	6	31	dep	followed	825:832	arg1	VI					821:822	VI	821:822	VI	821:822	The sorption kinetic analysis revealed that sorption of Cu (II) and U (VI) followed the pseudo-second-order model well and exhibited 3-stage intraparticle diffusion model during the whole sorption process.
28938140	0	32	theme	calcium	25:31	arg1	beads					81:85	calcium alginate coated chitosan hydrochloride hydrogel beads	25:85	calcium alginate coated chitosan hydrochloride hydrogel beads	25:85	Encapsulating Fe3O4 into calcium alginate coated chitosan hydrochloride hydrogel beads for removal of Cu (II) and U (VI) from aqueous solutions.
28938140	3	33	theme	EDX	469:471	arg1	spectra					473:479	EDX spectra	469:479	EDX spectra	469:479	SEM images and EDX spectra showed that Cu2+ and UO22+ ions were adhered onto sorbents.
28938140	9	34	dep	ions	1414:1417	arg1	VI					1410:1411	VI	1410:1411	VI	1410:1411	The results demonstrated that CCM can be an effective and promising sorbent for Cu (II) and U (VI) ions in wastewater.
28938140	4	35	theme	Adsorption	541:550	arg1	properties					552:561	Adsorption properties	541:561	Adsorption properties for removal of both copper and uranium ions under various experimental conditions	541:643	Adsorption properties for removal of both copper and uranium ions under various experimental conditions were investigated.
28938140	1	36	theme	U	206:206	arg1	removal					183:189	the removal	179:189	the removal of Cu (II) and U (VI) ions from aqueous solutions	179:239	The aim of this work was to study the removal of Cu (II) and U (VI) ions from aqueous solutions by encapsulating magnetic Fe3O4 nanoparticles into calcium alginate coated chitosan hydrochloride (CCM) hydrogel beads.
28938140	7	37	theme	Langmuir	996:1003	arg1	model					1005:1009	Langmuir model	996:1009	Langmuir model	996:1009	Equilibrium data were best described by Langmuir model, and the CCM composite hydrogel beads showed the estimated maximum adsorption capacity 143.276mg/g and 392.692mg/g for Cu (II) and U (VI), respectively.
28938140	1	38	theme	calcium	292:298	arg1	beads					354:358	calcium alginate coated chitosan hydrochloride (CCM) hydrogel beads	292:358	calcium alginate coated chitosan hydrochloride (CCM) hydrogel beads	292:358	The aim of this work was to study the removal of Cu (II) and U (VI) ions from aqueous solutions by encapsulating magnetic Fe3O4 nanoparticles into calcium alginate coated chitosan hydrochloride (CCM) hydrogel beads.
28938140	8	39	theme	cycles	1223:1228	arg1	use					1230:1232	five cycles use	1218:1232	five cycles use	1218:1232	The CCM adsorbent exhibited excellent reusability for five cycles use without significant changes in the adsorption capacity and structural stability.
28938140	2	40	theme	XRD	374:376	arg1	data					387:390	ATR-FTIR and XRD analysis data	361:390	ATR-FTIR and XRD analysis data	361:390	ATR-FTIR and XRD analysis data indicated that the CCM composites were successfully prepared.
28938140	9	41	theme	promising	1373:1381	arg1	sorbent					1383:1389	an effective and promising sorbent	1356:1389	an effective and promising sorbent for Cu (II) and U (VI) ions in wastewater	1356:1431	The results demonstrated that CCM can be an effective and promising sorbent for Cu (II) and U (VI) ions in wastewater.
28938140	9	41	theme	promising	1373:1381	arg1	CCM					1345:1347	CCM	1345:1347	CCM	1345:1347	The results demonstrated that CCM can be an effective and promising sorbent for Cu (II) and U (VI) ions in wastewater.
28938140	0	42	theme	coated	42:47	arg1	beads					81:85	calcium alginate coated chitosan hydrochloride hydrogel beads	25:85	calcium alginate coated chitosan hydrochloride hydrogel beads	25:85	Encapsulating Fe3O4 into calcium alginate coated chitosan hydrochloride hydrogel beads for removal of Cu (II) and U (VI) from aqueous solutions.
28938140	0	43	from	solutions	134:142	arg1	removal					91:97	removal	91:97	removal of Cu (II) and U (VI) from aqueous solutions	91:142	Encapsulating Fe3O4 into calcium alginate coated chitosan hydrochloride hydrogel beads for removal of Cu (II) and U (VI) from aqueous solutions.
28938140	1	44	theme	alginate	300:307	arg1	beads					354:358	calcium alginate coated chitosan hydrochloride (CCM) hydrogel beads	292:358	calcium alginate coated chitosan hydrochloride (CCM) hydrogel beads	292:358	The aim of this work was to study the removal of Cu (II) and U (VI) ions from aqueous solutions by encapsulating magnetic Fe3O4 nanoparticles into calcium alginate coated chitosan hydrochloride (CCM) hydrogel beads.
28938140	3	45	theme	UO22+	502:506	arg1	ions					508:511	Cu2+ and UO22+ ions	493:511	ions	508:511	SEM images and EDX spectra showed that Cu2+ and UO22+ ions were adhered onto sorbents.
28938140	9	46	theme	effective	1359:1367	arg1	sorbent					1383:1389	an effective and promising sorbent	1356:1389	an effective and promising sorbent for Cu (II) and U (VI) ions in wastewater	1356:1431	The results demonstrated that CCM can be an effective and promising sorbent for Cu (II) and U (VI) ions in wastewater.
28938140	9	46	theme	effective	1359:1367	arg1	CCM					1345:1347	CCM	1345:1347	CCM	1345:1347	The results demonstrated that CCM can be an effective and promising sorbent for Cu (II) and U (VI) ions in wastewater.
28938140	1	47	theme	work	161:164	arg1	aim					149:151	The aim	145:151	The aim of this work	145:164	The aim of this work was to study the removal of Cu (II) and U (VI) ions from aqueous solutions by encapsulating magnetic Fe3O4 nanoparticles into calcium alginate coated chitosan hydrochloride (CCM) hydrogel beads.
28938140	0	48	theme	alginate	33:40	arg1	beads					81:85	calcium alginate coated chitosan hydrochloride hydrogel beads	25:85	calcium alginate coated chitosan hydrochloride hydrogel beads	25:85	Encapsulating Fe3O4 into calcium alginate coated chitosan hydrochloride hydrogel beads for removal of Cu (II) and U (VI) from aqueous solutions.
28938140	0	49	theme	U	114:114	arg1	removal					91:97	removal	91:97	removal of Cu (II) and U (VI) from aqueous solutions	91:142	Encapsulating Fe3O4 into calcium alginate coated chitosan hydrochloride hydrogel beads for removal of Cu (II) and U (VI) from aqueous solutions.
28938140	1	50	theme	coated	309:314	arg1	beads					354:358	calcium alginate coated chitosan hydrochloride (CCM) hydrogel beads	292:358	calcium alginate coated chitosan hydrochloride (CCM) hydrogel beads	292:358	The aim of this work was to study the removal of Cu (II) and U (VI) ions from aqueous solutions by encapsulating magnetic Fe3O4 nanoparticles into calcium alginate coated chitosan hydrochloride (CCM) hydrogel beads.
28938140	7	51	theme	adsorption	1078:1087	arg1	capacity					1089:1096	the estimated maximum adsorption capacity 143.276mg/g and 392.692mg/g	1056:1124	capacity	1089:1096	Equilibrium data were best described by Langmuir model, and the CCM composite hydrogel beads showed the estimated maximum adsorption capacity 143.276mg/g and 392.692mg/g for Cu (II) and U (VI), respectively.
28938140	7	52	dep	capacity	1089:1096	arg1	143.276mg/g					1098:1108	143.276mg/g	1098:1108	143.276mg/g	1098:1108	Equilibrium data were best described by Langmuir model, and the CCM composite hydrogel beads showed the estimated maximum adsorption capacity 143.276mg/g and 392.692mg/g for Cu (II) and U (VI), respectively.
28938140	0	53	theme	hydrochloride	58:70	arg1	beads					81:85	calcium alginate coated chitosan hydrochloride hydrogel beads	25:85	calcium alginate coated chitosan hydrochloride hydrogel beads	25:85	Encapsulating Fe3O4 into calcium alginate coated chitosan hydrochloride hydrogel beads for removal of Cu (II) and U (VI) from aqueous solutions.
28938140	6	54	theme	pseudo-second-order	838:856	arg1	model					858:862	the pseudo-second-order model	834:862	the pseudo-second-order model	834:862	The sorption kinetic analysis revealed that sorption of Cu (II) and U (VI) followed the pseudo-second-order model well and exhibited 3-stage intraparticle diffusion model during the whole sorption process.
28938140	1	55	theme	chitosan	316:323	arg1	beads					354:358	calcium alginate coated chitosan hydrochloride (CCM) hydrogel beads	292:358	calcium alginate coated chitosan hydrochloride (CCM) hydrogel beads	292:358	The aim of this work was to study the removal of Cu (II) and U (VI) ions from aqueous solutions by encapsulating magnetic Fe3O4 nanoparticles into calcium alginate coated chitosan hydrochloride (CCM) hydrogel beads.
28938140	3	56	theme	Cu2+	493:496	arg1	ions					508:511	Cu2+ and UO22+ ions	493:511	ions	508:511	SEM images and EDX spectra showed that Cu2+ and UO22+ ions were adhered onto sorbents.
28938140	4	57	theme	experimental	621:632	arg1	conditions					634:643	various experimental conditions	613:643	various experimental conditions	613:643	Adsorption properties for removal of both copper and uranium ions under various experimental conditions were investigated.
28938140	0	58	theme	chitosan	49:56	arg1	beads					81:85	calcium alginate coated chitosan hydrochloride hydrogel beads	25:85	calcium alginate coated chitosan hydrochloride hydrogel beads	25:85	Encapsulating Fe3O4 into calcium alginate coated chitosan hydrochloride hydrogel beads for removal of Cu (II) and U (VI) from aqueous solutions.
28938140	1	59	dep	U	206:206	arg1	ions					213:216	ions	213:216	U (VI) ions	206:216	The aim of this work was to study the removal of Cu (II) and U (VI) ions from aqueous solutions by encapsulating magnetic Fe3O4 nanoparticles into calcium alginate coated chitosan hydrochloride (CCM) hydrogel beads.
28938140	1	59	dep	U	206:206	arg1	VI					209:210	VI	209:210	VI	209:210	The aim of this work was to study the removal of Cu (II) and U (VI) ions from aqueous solutions by encapsulating magnetic Fe3O4 nanoparticles into calcium alginate coated chitosan hydrochloride (CCM) hydrogel beads.
28938140	1	60	theme	hydrochloride	325:337	arg1	beads					354:358	calcium alginate coated chitosan hydrochloride (CCM) hydrogel beads	292:358	calcium alginate coated chitosan hydrochloride (CCM) hydrogel beads	292:358	The aim of this work was to study the removal of Cu (II) and U (VI) ions from aqueous solutions by encapsulating magnetic Fe3O4 nanoparticles into calcium alginate coated chitosan hydrochloride (CCM) hydrogel beads.
28938140	4	61	theme	various	613:619	arg1	conditions					634:643	various experimental conditions	613:643	various experimental conditions	613:643	Adsorption properties for removal of both copper and uranium ions under various experimental conditions were investigated.
28938140	0	62	theme	aqueous	126:132	arg1	solutions					134:142	aqueous solutions	126:142	aqueous solutions	126:142	Encapsulating Fe3O4 into calcium alginate coated chitosan hydrochloride hydrogel beads for removal of Cu (II) and U (VI) from aqueous solutions.
28938140	5	63	theme	sorption	681:688	arg1	isotherms					702:710	sorption equilibrium isotherms	681:710	sorption equilibrium isotherms	681:710	Kinetic data and sorption equilibrium isotherms were also conducted in batch process.
28938140	8	64	from	changes	1254:1260	arg1	capacity					1280:1287	the adsorption capacity	1265:1287	the adsorption capacity	1265:1287	The CCM adsorbent exhibited excellent reusability for five cycles use without significant changes in the adsorption capacity and structural stability.
28938140	8	64	from	changes	1254:1260	arg1	stability					1304:1312	structural stability	1293:1312	structural stability	1293:1312	The CCM adsorbent exhibited excellent reusability for five cycles use without significant changes in the adsorption capacity and structural stability.
28938140	3	65	theme	SEM	454:456	arg1	images					458:463	SEM images	454:463	SEM images	454:463	SEM images and EDX spectra showed that Cu2+ and UO22+ ions were adhered onto sorbents.
28938140	1	66	theme	CCM	340:342	arg1	beads					354:358	calcium alginate coated chitosan hydrochloride (CCM) hydrogel beads	292:358	calcium alginate coated chitosan hydrochloride (CCM) hydrogel beads	292:358	The aim of this work was to study the removal of Cu (II) and U (VI) ions from aqueous solutions by encapsulating magnetic Fe3O4 nanoparticles into calcium alginate coated chitosan hydrochloride (CCM) hydrogel beads.
28938140	8	67	theme	excellent	1192:1200	arg1	reusability					1202:1212	excellent reusability	1192:1212	excellent reusability for five cycles use	1192:1232	The CCM adsorbent exhibited excellent reusability for five cycles use without significant changes in the adsorption capacity and structural stability.
28938140	6	68	theme	sorption	938:945	arg1	process					947:953	the whole sorption process	928:953	the whole sorption process	928:953	The sorption kinetic analysis revealed that sorption of Cu (II) and U (VI) followed the pseudo-second-order model well and exhibited 3-stage intraparticle diffusion model during the whole sorption process.
28938140	2	69	theme	CCM	411:413	arg1	composites					415:424	the CCM composites	407:424	the CCM composites	407:424	ATR-FTIR and XRD analysis data indicated that the CCM composites were successfully prepared.
28938140	6	70	theme	sorption	754:761	arg1	analysis					771:778	The sorption kinetic analysis	750:778	The sorption kinetic analysis	750:778	The sorption kinetic analysis revealed that sorption of Cu (II) and U (VI) followed the pseudo-second-order model well and exhibited 3-stage intraparticle diffusion model during the whole sorption process.
28938140	4	71	theme	uranium	594:600	arg1	ions					602:605	uranium ions	594:605	uranium ions	594:605	Adsorption properties for removal of both copper and uranium ions under various experimental conditions were investigated.
28938140	7	72	theme	maximum	1070:1076	arg1	capacity					1089:1096	the estimated maximum adsorption capacity 143.276mg/g and 392.692mg/g	1056:1124	capacity	1089:1096	Equilibrium data were best described by Langmuir model, and the CCM composite hydrogel beads showed the estimated maximum adsorption capacity 143.276mg/g and 392.692mg/g for Cu (II) and U (VI), respectively.
28938140	6	73	theme	whole	932:936	arg1	process					947:953	the whole sorption process	928:953	the whole sorption process	928:953	The sorption kinetic analysis revealed that sorption of Cu (II) and U (VI) followed the pseudo-second-order model well and exhibited 3-stage intraparticle diffusion model during the whole sorption process.
28938140	0	74	theme	hydrogel	72:79	arg1	beads					81:85	calcium alginate coated chitosan hydrochloride hydrogel beads	25:85	calcium alginate coated chitosan hydrochloride hydrogel beads	25:85	Encapsulating Fe3O4 into calcium alginate coated chitosan hydrochloride hydrogel beads for removal of Cu (II) and U (VI) from aqueous solutions.
28938140	1	75	theme	aqueous	223:229	arg1	solutions					231:239	aqueous solutions	223:239	aqueous solutions	223:239	The aim of this work was to study the removal of Cu (II) and U (VI) ions from aqueous solutions by encapsulating magnetic Fe3O4 nanoparticles into calcium alginate coated chitosan hydrochloride (CCM) hydrogel beads.
28938140	0	76	dep	U	114:114	arg1	VI					117:118	VI	117:118	VI	117:118	Encapsulating Fe3O4 into calcium alginate coated chitosan hydrochloride hydrogel beads for removal of Cu (II) and U (VI) from aqueous solutions.
25296366	7	0	theme	inclusion	1221:1229	arg1	formation					1244:1252	their inclusion complex (IC) formation	1215:1252	their inclusion complex (IC) formation with CDs	1215:1261	Phenolphthalein absorption tests were performed to study the kinetics of their inclusion complex (IC) formation with CDs.
25296366	10	1	from	applications	1594:1605	arg1	particular					1611:1620	particular	1611:1620	particular	1611:1620	With their encapsulation capabilities and their lowered hydrophobicity, PCL/CD composite fibers might have potential uses in medical applications, in particular as wound odor absorbants in dressings, because it is well known that CDs can form ICs with these odorants, thereby effectively removing them.
25296366	10	2	theme	wound	1625:1629	arg1	absorbants					1636:1645	wound odor absorbants	1625:1645	wound odor absorbants in dressings	1625:1658	With their encapsulation capabilities and their lowered hydrophobicity, PCL/CD composite fibers might have potential uses in medical applications, in particular as wound odor absorbants in dressings, because it is well known that CDs can form ICs with these odorants, thereby effectively removing them.
25296366	5	3	theme	composite	821:829	arg1	fibers					831:836	the PCL/CD composite fibers	810:836	the PCL/CD composite fibers	810:836	Differential scanning calorimetry showed that the PCL/CD composite fibers exhibit higher crystallization temperatures and sharper crystallization exotherms with increased CD loading, indicating the ability of CDs to nucleate PCL crystallization.
25296366	6	4	theme	γ-CD	1104:1107	arg1	concentration					1109:1121	γ-CD concentration	1104:1121	γ-CD concentration	1104:1121	Water contact angle (WCA) measurements indicate an inverse relationship between WCA and α- or γ-CD concentration up to 30% loading.
25296366	6	5	theme	angle	1024:1028	arg1	measurements					1036:1047	Water contact angle (WCA) measurements	1010:1047	Water contact angle (WCA) measurements	1010:1047	Water contact angle (WCA) measurements indicate an inverse relationship between WCA and α- or γ-CD concentration up to 30% loading.
25296366	5	6	theme	CDs	973:975	arg1	ability					962:968	the ability	958:968	the ability of CDs to nucleate PCL crystallization	958:1007	Differential scanning calorimetry showed that the PCL/CD composite fibers exhibit higher crystallization temperatures and sharper crystallization exotherms with increased CD loading, indicating the ability of CDs to nucleate PCL crystallization.
25296366	7	7	with	formation	1244:1252	arg1	CDs					1259:1261	CDs	1259:1261	CDs	1259:1261	Phenolphthalein absorption tests were performed to study the kinetics of their inclusion complex (IC) formation with CDs.
25296366	5	8	theme	increased	925:933	arg1	loading					938:944	increased CD loading	925:944	increased CD loading	925:944	Differential scanning calorimetry showed that the PCL/CD composite fibers exhibit higher crystallization temperatures and sharper crystallization exotherms with increased CD loading, indicating the ability of CDs to nucleate PCL crystallization.
25296366	6	9	theme	contact	1016:1022	arg1	WCA					1031:1033	WCA	1031:1033	WCA	1031:1033	Water contact angle (WCA) measurements indicate an inverse relationship between WCA and α- or γ-CD concentration up to 30% loading.
25296366	6	9	theme	contact	1016:1022	arg1	angle					1024:1028	Water contact angle	1010:1028	Water contact angle (WCA) measurements	1010:1047	Water contact angle (WCA) measurements indicate an inverse relationship between WCA and α- or γ-CD concentration up to 30% loading.
25296366	10	10	theme	medical	1586:1592	arg1	applications					1594:1605	medical applications	1586:1605	medical applications	1586:1605	With their encapsulation capabilities and their lowered hydrophobicity, PCL/CD composite fibers might have potential uses in medical applications, in particular as wound odor absorbants in dressings, because it is well known that CDs can form ICs with these odorants, thereby effectively removing them.
25296366	10	11	theme	lowered	1509:1515	arg1	hydrophobicity					1517:1530	their lowered hydrophobicity	1503:1530	their lowered hydrophobicity	1503:1530	With their encapsulation capabilities and their lowered hydrophobicity, PCL/CD composite fibers might have potential uses in medical applications, in particular as wound odor absorbants in dressings, because it is well known that CDs can form ICs with these odorants, thereby effectively removing them.
25296366	2	12	theme	PCL/CD	259:264	arg1	nanofibers					276:285	PCL/CD composite nanofibers	259:285	PCL/CD composite nanofibers	259:285	PCL/CD composite nanofibers were obtained for the first time by electrospinning the mixture from chloroform/N,N-dimethylformamide (60:40).
25296366	4	13	dep	transform	660:668	arg1	infrared					670:677	infrared	670:677	transform infrared spectroscopy, thermogravimetric analysis, and wide-angle X-ray diffraction analysis	660:761	The presence of CDs on or in the electrospun PCL fibers in the electrospun mats was investigated using Fourier transform infrared spectroscopy, thermogravimetric analysis, and wide-angle X-ray diffraction analysis.
25296366	1	14	theme	nanofibers	224:233	arg1	thermal					142:148	thermal	142:148	thermal	142:148	The effects of alpha- and gamma-cyclodextrins (α- and γ-CDs) on the thermal and crystal nucleation behavior of electrospun poly(ε-caprolactone) (PCL) nanofibers have been investigated.
25296366	4	15	from	presence	553:560	arg1	or					572:573	or	572:573	or	572:573	The presence of CDs on or in the electrospun PCL fibers in the electrospun mats was investigated using Fourier transform infrared spectroscopy, thermogravimetric analysis, and wide-angle X-ray diffraction analysis.
25296366	3	16	contain	have	471:474	arg1	nanofibers					460:469	neat PCL nanofibers	451:469	neat PCL nanofibers	451:469	Scanning electron microscopy analyses indicated that neat PCL nanofibers have an average diameter of 400 nm, which increases with the addition of CDs.
25296366	3	16	contain	have	471:474	arg2	diameter					487:494	an average diameter	476:494	an average diameter	476:494	Scanning electron microscopy analyses indicated that neat PCL nanofibers have an average diameter of 400 nm, which increases with the addition of CDs.
25296366	7	17	theme	formation	1244:1252	arg1	kinetics					1203:1210	the kinetics	1199:1210	the kinetics of their inclusion complex (IC) formation with CDs	1199:1261	Phenolphthalein absorption tests were performed to study the kinetics of their inclusion complex (IC) formation with CDs.
25296366	4	18	theme	wide-angle	725:734	arg1	analysis					754:761	wide-angle X-ray diffraction analysis	725:761	wide-angle X-ray diffraction analysis	725:761	The presence of CDs on or in the electrospun PCL fibers in the electrospun mats was investigated using Fourier transform infrared spectroscopy, thermogravimetric analysis, and wide-angle X-ray diffraction analysis.
25296366	5	19	theme	CD	935:936	arg1	loading					938:944	increased CD loading	925:944	increased CD loading	925:944	Differential scanning calorimetry showed that the PCL/CD composite fibers exhibit higher crystallization temperatures and sharper crystallization exotherms with increased CD loading, indicating the ability of CDs to nucleate PCL crystallization.
25296366	6	20	theme	inverse	1061:1067	arg1	relationship					1069:1080	an inverse relationship	1058:1080	an inverse relationship between WCA and α- or γ-CD concentration up to 30% loading	1058:1139	Water contact angle (WCA) measurements indicate an inverse relationship between WCA and α- or γ-CD concentration up to 30% loading.
25296366	5	21	theme	PCL	989:991	arg1	crystallization					993:1007	PCL crystallization	989:1007	PCL crystallization	989:1007	Differential scanning calorimetry showed that the PCL/CD composite fibers exhibit higher crystallization temperatures and sharper crystallization exotherms with increased CD loading, indicating the ability of CDs to nucleate PCL crystallization.
25296366	1	22	theme	crystal	154:160	arg1	behavior					173:180	the thermal and crystal nucleation behavior	138:180	behavior	173:180	The effects of alpha- and gamma-cyclodextrins (α- and γ-CDs) on the thermal and crystal nucleation behavior of electrospun poly(ε-caprolactone) (PCL) nanofibers have been investigated.
25296366	3	23	theme	microscopy	416:425	arg1	analyses					427:434	Scanning electron microscopy analyses	398:434	Scanning electron microscopy analyses	398:434	Scanning electron microscopy analyses indicated that neat PCL nanofibers have an average diameter of 400 nm, which increases with the addition of CDs.
25296366	10	24	from	absorbants	1636:1645	arg1	dressings					1650:1658	dressings	1650:1658	dressings	1650:1658	With their encapsulation capabilities and their lowered hydrophobicity, PCL/CD composite fibers might have potential uses in medical applications, in particular as wound odor absorbants in dressings, because it is well known that CDs can form ICs with these odorants, thereby effectively removing them.
25296366	10	25	theme	encapsulation	1472:1484	arg1	capabilities					1486:1497	their encapsulation capabilities	1466:1497	their encapsulation capabilities	1466:1497	With their encapsulation capabilities and their lowered hydrophobicity, PCL/CD composite fibers might have potential uses in medical applications, in particular as wound odor absorbants in dressings, because it is well known that CDs can form ICs with these odorants, thereby effectively removing them.
25296366	4	26	theme	diffraction	742:752	arg1	analysis					754:761	wide-angle X-ray diffraction analysis	725:761	wide-angle X-ray diffraction analysis	725:761	The presence of CDs on or in the electrospun PCL fibers in the electrospun mats was investigated using Fourier transform infrared spectroscopy, thermogravimetric analysis, and wide-angle X-ray diffraction analysis.
25296366	5	27	theme	higher	846:851	arg1	temperatures					869:880	higher crystallization temperatures	846:880	higher crystallization temperatures	846:880	Differential scanning calorimetry showed that the PCL/CD composite fibers exhibit higher crystallization temperatures and sharper crystallization exotherms with increased CD loading, indicating the ability of CDs to nucleate PCL crystallization.
25296366	7	28	theme	complex	1231:1237	arg1	formation					1244:1252	their inclusion complex (IC) formation	1215:1252	their inclusion complex (IC) formation with CDs	1215:1261	Phenolphthalein absorption tests were performed to study the kinetics of their inclusion complex (IC) formation with CDs.
25296366	6	29	dep	WCA	1090:1092	arg1	loading					1133:1139	up to 30% loading	1123:1139	up to 30% loading	1123:1139	Water contact angle (WCA) measurements indicate an inverse relationship between WCA and α- or γ-CD concentration up to 30% loading.
25296366	3	30	theme	Scanning	398:405	arg1	microscopy					416:425	Scanning electron microscopy	398:425	Scanning electron microscopy analyses	398:434	Scanning electron microscopy analyses indicated that neat PCL nanofibers have an average diameter of 400 nm, which increases with the addition of CDs.
25296366	9	31	theme	formed	1427:1432	arg1	ICs					1434:1436	formed ICs	1427:1436	formed ICs	1427:1436	This might be because at elevated loadings some α-CDs may have threaded over PCL chains and formed ICs, whereas γ-CD did not.
25296366	3	32	theme	CDs	544:546	arg1	addition					532:539	the addition	528:539	the addition of CDs	528:546	Scanning electron microscopy analyses indicated that neat PCL nanofibers have an average diameter of 400 nm, which increases with the addition of CDs.
25296366	4	33	from	fibers	598:603	arg1	mats					624:627	the electrospun mats	608:627	the electrospun mats	608:627	The presence of CDs on or in the electrospun PCL fibers in the electrospun mats was investigated using Fourier transform infrared spectroscopy, thermogravimetric analysis, and wide-angle X-ray diffraction analysis.
25296366	1	34	theme	nucleation	162:171	arg1	behavior					173:180	the thermal and crystal nucleation behavior	138:180	behavior	173:180	The effects of alpha- and gamma-cyclodextrins (α- and γ-CDs) on the thermal and crystal nucleation behavior of electrospun poly(ε-caprolactone) (PCL) nanofibers have been investigated.
25296366	6	35	dep	loading	1133:1139	arg1	%					1131:1131	%	1131:1131	%	1131:1131	Water contact angle (WCA) measurements indicate an inverse relationship between WCA and α- or γ-CD concentration up to 30% loading.
25296366	1	36	theme	alpha-	89:94	arg1	effects					78:84	The effects	74:84	The effects of alpha- and gamma-cyclodextrins (α- and γ-CDs) on the thermal and crystal nucleation behavior of electrospun poly(ε-caprolactone) (PCL) nanofibers	74:233	The effects of alpha- and gamma-cyclodextrins (α- and γ-CDs) on the thermal and crystal nucleation behavior of electrospun poly(ε-caprolactone) (PCL) nanofibers have been investigated.
25296366	3	37	theme	neat	451:454	arg1	nanofibers					460:469	neat PCL nanofibers	451:469	neat PCL nanofibers	451:469	Scanning electron microscopy analyses indicated that neat PCL nanofibers have an average diameter of 400 nm, which increases with the addition of CDs.
25296366	4	38	theme	thermogravimetric	693:709	arg1	analysis					711:718	thermogravimetric analysis	693:718	thermogravimetric analysis	693:718	The presence of CDs on or in the electrospun PCL fibers in the electrospun mats was investigated using Fourier transform infrared spectroscopy, thermogravimetric analysis, and wide-angle X-ray diffraction analysis.
25296366	10	39	dep	potential	1568:1576	arg1	uses					1578:1581	uses	1578:1581	uses in medical applications, in particular as wound odor absorbants in dressings	1578:1658	With their encapsulation capabilities and their lowered hydrophobicity, PCL/CD composite fibers might have potential uses in medical applications, in particular as wound odor absorbants in dressings, because it is well known that CDs can form ICs with these odorants, thereby effectively removing them.
25296366	2	40	theme	composite	266:274	arg1	nanofibers					276:285	PCL/CD composite nanofibers	259:285	PCL/CD composite nanofibers	259:285	PCL/CD composite nanofibers were obtained for the first time by electrospinning the mixture from chloroform/N,N-dimethylformamide (60:40).
25296366	3	41	theme	PCL	456:458	arg1	nanofibers					460:469	neat PCL nanofibers	451:469	neat PCL nanofibers	451:469	Scanning electron microscopy analyses indicated that neat PCL nanofibers have an average diameter of 400 nm, which increases with the addition of CDs.
25296366	7	42	theme	absorption	1158:1167	arg1	tests					1169:1173	Phenolphthalein absorption tests	1142:1173	Phenolphthalein absorption tests	1142:1173	Phenolphthalein absorption tests were performed to study the kinetics of their inclusion complex (IC) formation with CDs.
25296366	3	43	theme	average	479:485	arg1	diameter					487:494	an average diameter	476:494	an average diameter	476:494	Scanning electron microscopy analyses indicated that neat PCL nanofibers have an average diameter of 400 nm, which increases with the addition of CDs.
25296366	8	44	theme	γ-CD-functionalized	1278:1296	arg1	nanowebs					1298:1305	γ-CD-functionalized nanowebs	1278:1305	γ-CD-functionalized nanowebs	1278:1305	Unexpectedly, γ-CD-functionalized nanowebs performed better than α-CD.
25296366	3	45	theme	electron	407:414	arg1	microscopy					416:425	Scanning electron microscopy	398:425	Scanning electron microscopy analyses	398:434	Scanning electron microscopy analyses indicated that neat PCL nanofibers have an average diameter of 400 nm, which increases with the addition of CDs.
25296366	1	46	theme	electrospun	185:195	arg1	ε-caprolactone					202:215	ε-caprolactone	202:215	ε-caprolactone	202:215	The effects of alpha- and gamma-cyclodextrins (α- and γ-CDs) on the thermal and crystal nucleation behavior of electrospun poly(ε-caprolactone) (PCL) nanofibers have been investigated.
25296366	1	46	theme	electrospun	185:195	arg1	PCL					219:221	PCL	219:221	PCL	219:221	The effects of alpha- and gamma-cyclodextrins (α- and γ-CDs) on the thermal and crystal nucleation behavior of electrospun poly(ε-caprolactone) (PCL) nanofibers have been investigated.
25296366	1	46	theme	electrospun	185:195	arg1	poly					197:200	electrospun poly	185:200	electrospun poly(ε-caprolactone) (PCL) nanofibers	185:233	The effects of alpha- and gamma-cyclodextrins (α- and γ-CDs) on the thermal and crystal nucleation behavior of electrospun poly(ε-caprolactone) (PCL) nanofibers have been investigated.
25296366	5	47	theme	crystallization	853:867	arg1	temperatures					869:880	higher crystallization temperatures	846:880	higher crystallization temperatures	846:880	Differential scanning calorimetry showed that the PCL/CD composite fibers exhibit higher crystallization temperatures and sharper crystallization exotherms with increased CD loading, indicating the ability of CDs to nucleate PCL crystallization.
25296366	5	48	theme	Differential	764:775	arg1	calorimetry					786:796	Differential scanning calorimetry	764:796	Differential scanning calorimetry	764:796	Differential scanning calorimetry showed that the PCL/CD composite fibers exhibit higher crystallization temperatures and sharper crystallization exotherms with increased CD loading, indicating the ability of CDs to nucleate PCL crystallization.
25296366	2	49	from	chloroform/N	356:367	arg1	mixture					343:349	the mixture	339:349	the mixture from chloroform/N,N-dimethylformamide (60:40)	339:395	PCL/CD composite nanofibers were obtained for the first time by electrospinning the mixture from chloroform/N,N-dimethylformamide (60:40).
25296366	1	50	theme	poly	197:200	arg1	nanofibers					224:233	electrospun poly(ε-caprolactone) (PCL) nanofibers	185:233	electrospun poly(ε-caprolactone) (PCL) nanofibers	185:233	The effects of alpha- and gamma-cyclodextrins (α- and γ-CDs) on the thermal and crystal nucleation behavior of electrospun poly(ε-caprolactone) (PCL) nanofibers have been investigated.
25296366	5	51	theme	scanning	777:784	arg1	calorimetry					786:796	Differential scanning calorimetry	764:796	Differential scanning calorimetry	764:796	Differential scanning calorimetry showed that the PCL/CD composite fibers exhibit higher crystallization temperatures and sharper crystallization exotherms with increased CD loading, indicating the ability of CDs to nucleate PCL crystallization.
25296366	5	52	theme	crystallization	894:908	arg1	exotherms					910:918	sharper crystallization exotherms	886:918	sharper crystallization exotherms	886:918	Differential scanning calorimetry showed that the PCL/CD composite fibers exhibit higher crystallization temperatures and sharper crystallization exotherms with increased CD loading, indicating the ability of CDs to nucleate PCL crystallization.
25296366	10	53	theme	PCL/CD	1533:1538	arg1	fibers					1550:1555	PCL/CD composite fibers	1533:1555	PCL/CD composite fibers	1533:1555	With their encapsulation capabilities and their lowered hydrophobicity, PCL/CD composite fibers might have potential uses in medical applications, in particular as wound odor absorbants in dressings, because it is well known that CDs can form ICs with these odorants, thereby effectively removing them.
25296366	7	54	theme	IC	1240:1241	arg1	formation					1244:1252	their inclusion complex (IC) formation	1215:1252	their inclusion complex (IC) formation with CDs	1215:1261	Phenolphthalein absorption tests were performed to study the kinetics of their inclusion complex (IC) formation with CDs.
25296366	1	55	theme	gamma-cyclodextrins	100:118	arg1	effects					78:84	The effects	74:84	The effects of alpha- and gamma-cyclodextrins (α- and γ-CDs) on the thermal and crystal nucleation behavior of electrospun poly(ε-caprolactone) (PCL) nanofibers	74:233	The effects of alpha- and gamma-cyclodextrins (α- and γ-CDs) on the thermal and crystal nucleation behavior of electrospun poly(ε-caprolactone) (PCL) nanofibers have been investigated.
25296366	7	56	theme	Phenolphthalein	1142:1156	arg1	tests					1169:1173	Phenolphthalein absorption tests	1142:1173	Phenolphthalein absorption tests	1142:1173	Phenolphthalein absorption tests were performed to study the kinetics of their inclusion complex (IC) formation with CDs.
25296366	0	57	dep	nanowebs	21:28	arg1	functionalized					30:43	functionalized	30:43	nanowebs functionalized with α- and γ-cyclodextrins	21:71	Poly(ε-caprolactone) nanowebs functionalized with α- and γ-cyclodextrins.
25296366	2	58	theme	first	309:313	arg1	time					315:318	the first time	305:318	the first time	305:318	PCL/CD composite nanofibers were obtained for the first time by electrospinning the mixture from chloroform/N,N-dimethylformamide (60:40).
25296366	6	59	dep	%	1131:1131	arg1	30					1129:1130	30	1129:1130	30	1129:1130	Water contact angle (WCA) measurements indicate an inverse relationship between WCA and α- or γ-CD concentration up to 30% loading.
25296366	4	60	theme	electrospun	612:622	arg1	mats					624:627	the electrospun mats	608:627	the electrospun mats	608:627	The presence of CDs on or in the electrospun PCL fibers in the electrospun mats was investigated using Fourier transform infrared spectroscopy, thermogravimetric analysis, and wide-angle X-ray diffraction analysis.
25296366	6	61	dep	30	1129:1130	arg1	to					1126:1127	to	1126:1127	to	1126:1127	Water contact angle (WCA) measurements indicate an inverse relationship between WCA and α- or γ-CD concentration up to 30% loading.
25296366	5	62	theme	sharper	886:892	arg1	exotherms					910:918	sharper crystallization exotherms	886:918	sharper crystallization exotherms	886:918	Differential scanning calorimetry showed that the PCL/CD composite fibers exhibit higher crystallization temperatures and sharper crystallization exotherms with increased CD loading, indicating the ability of CDs to nucleate PCL crystallization.
25296366	10	63	contain	have	1563:1566	arg1	fibers					1550:1555	PCL/CD composite fibers	1533:1555	PCL/CD composite fibers	1533:1555	With their encapsulation capabilities and their lowered hydrophobicity, PCL/CD composite fibers might have potential uses in medical applications, in particular as wound odor absorbants in dressings, because it is well known that CDs can form ICs with these odorants, thereby effectively removing them.
25296366	10	63	contain	have	1563:1566	arg2	potential					1568:1576	potential	1568:1576	potential uses in medical applications, in particular as wound odor absorbants in dressings	1568:1658	With their encapsulation capabilities and their lowered hydrophobicity, PCL/CD composite fibers might have potential uses in medical applications, in particular as wound odor absorbants in dressings, because it is well known that CDs can form ICs with these odorants, thereby effectively removing them.
25296366	4	64	theme	X-ray	736:740	arg1	analysis					754:761	wide-angle X-ray diffraction analysis	725:761	wide-angle X-ray diffraction analysis	725:761	The presence of CDs on or in the electrospun PCL fibers in the electrospun mats was investigated using Fourier transform infrared spectroscopy, thermogravimetric analysis, and wide-angle X-ray diffraction analysis.
25296366	1	65	from	effects	78:84	arg1	thermal					142:148	thermal	142:148	thermal	142:148	The effects of alpha- and gamma-cyclodextrins (α- and γ-CDs) on the thermal and crystal nucleation behavior of electrospun poly(ε-caprolactone) (PCL) nanofibers have been investigated.
25296366	1	65	from	effects	78:84	arg1	behavior					173:180	the thermal and crystal nucleation behavior	138:180	behavior	173:180	The effects of alpha- and gamma-cyclodextrins (α- and γ-CDs) on the thermal and crystal nucleation behavior of electrospun poly(ε-caprolactone) (PCL) nanofibers have been investigated.
25296366	4	66	theme	CDs	565:567	arg1	presence					553:560	The presence	549:560	The presence of CDs on or in the electrospun PCL fibers in the electrospun mats	549:627	The presence of CDs on or in the electrospun PCL fibers in the electrospun mats was investigated using Fourier transform infrared spectroscopy, thermogravimetric analysis, and wide-angle X-ray diffraction analysis.
25296366	10	67	theme	odor	1631:1634	arg1	absorbants					1636:1645	wound odor absorbants	1625:1645	wound odor absorbants in dressings	1625:1658	With their encapsulation capabilities and their lowered hydrophobicity, PCL/CD composite fibers might have potential uses in medical applications, in particular as wound odor absorbants in dressings, because it is well known that CDs can form ICs with these odorants, thereby effectively removing them.
25296366	4	68	theme	PCL	594:596	arg1	fibers					598:603	the electrospun PCL fibers	578:603	the electrospun PCL fibers in the electrospun mats	578:627	The presence of CDs on or in the electrospun PCL fibers in the electrospun mats was investigated using Fourier transform infrared spectroscopy, thermogravimetric analysis, and wide-angle X-ray diffraction analysis.
25296366	4	69	attach	presence	553:560	arg1	or					572:573	or	572:573	or	572:573	The presence of CDs on or in the electrospun PCL fibers in the electrospun mats was investigated using Fourier transform infrared spectroscopy, thermogravimetric analysis, and wide-angle X-ray diffraction analysis.
25296366	4	69	attach	presence	553:560	arg2	CDs					565:567	CDs	565:567	CDs	565:567	The presence of CDs on or in the electrospun PCL fibers in the electrospun mats was investigated using Fourier transform infrared spectroscopy, thermogravimetric analysis, and wide-angle X-ray diffraction analysis.
25296366	4	70	theme	electrospun	582:592	arg1	fibers					598:603	the electrospun PCL fibers	578:603	the electrospun PCL fibers in the electrospun mats	578:627	The presence of CDs on or in the electrospun PCL fibers in the electrospun mats was investigated using Fourier transform infrared spectroscopy, thermogravimetric analysis, and wide-angle X-ray diffraction analysis.
25296366	1	71	dep	alpha-	89:94	arg1	α-					121:122	α-	121:122	α-	121:122	The effects of alpha- and gamma-cyclodextrins (α- and γ-CDs) on the thermal and crystal nucleation behavior of electrospun poly(ε-caprolactone) (PCL) nanofibers have been investigated.
25296366	1	71	dep	alpha-	89:94	arg1	γ-CDs					128:132	γ-CDs	128:132	γ-CDs	128:132	The effects of alpha- and gamma-cyclodextrins (α- and γ-CDs) on the thermal and crystal nucleation behavior of electrospun poly(ε-caprolactone) (PCL) nanofibers have been investigated.
25296366	9	72	theme	elevated	1360:1367	arg1	loadings					1369:1376	elevated loadings	1360:1376	elevated loadings	1360:1376	This might be because at elevated loadings some α-CDs may have threaded over PCL chains and formed ICs, whereas γ-CD did not.
25296366	10	73	theme	composite	1540:1548	arg1	fibers					1550:1555	PCL/CD composite fibers	1533:1555	PCL/CD composite fibers	1533:1555	With their encapsulation capabilities and their lowered hydrophobicity, PCL/CD composite fibers might have potential uses in medical applications, in particular as wound odor absorbants in dressings, because it is well known that CDs can form ICs with these odorants, thereby effectively removing them.
25296366	6	74	theme	Water	1010:1014	arg1	WCA					1031:1033	WCA	1031:1033	WCA	1031:1033	Water contact angle (WCA) measurements indicate an inverse relationship between WCA and α- or γ-CD concentration up to 30% loading.
25296366	6	74	theme	Water	1010:1014	arg1	angle					1024:1028	Water contact angle	1010:1028	Water contact angle (WCA) measurements	1010:1047	Water contact angle (WCA) measurements indicate an inverse relationship between WCA and α- or γ-CD concentration up to 30% loading.
25296366	5	75	theme	PCL/CD	814:819	arg1	fibers					831:836	the PCL/CD composite fibers	810:836	the PCL/CD composite fibers	810:836	Differential scanning calorimetry showed that the PCL/CD composite fibers exhibit higher crystallization temperatures and sharper crystallization exotherms with increased CD loading, indicating the ability of CDs to nucleate PCL crystallization.
25296366	3	76	theme	nm	503:504	arg1	diameter					487:494	an average diameter	476:494	an average diameter	476:494	Scanning electron microscopy analyses indicated that neat PCL nanofibers have an average diameter of 400 nm, which increases with the addition of CDs.
25296366	4	77	dep	Fourier	652:658	arg1	transform					660:668	transform	660:668	transform infrared spectroscopy, thermogravimetric analysis, and wide-angle X-ray diffraction analysis	660:761	The presence of CDs on or in the electrospun PCL fibers in the electrospun mats was investigated using Fourier transform infrared spectroscopy, thermogravimetric analysis, and wide-angle X-ray diffraction analysis.
25296366	9	78	theme	PCL	1412:1414	arg1	chains					1416:1421	PCL chains	1412:1421	PCL chains	1412:1421	This might be because at elevated loadings some α-CDs may have threaded over PCL chains and formed ICs, whereas γ-CD did not.
25306849	6	0	theme	×	1037:1037	arg1	-1					1066:1067	-1	1066:1067	-1	1066:1067	For determination of albumin, the fabricated MIP cryogel biosensor showed a high sensitivity with a wide linear range of 1.0 × 10(-4) to 1.0 × 10(1) mg L(-1) and a low limit of detection of 5.0 × 10(-5) mg L(-1) (S/N = 3).
25306849	6	0	theme	×	1037:1037	arg1	L					1064:1064	1.0 × 10(-4) to 1.0 × 10(1) mg L	1033:1064	1.0 × 10(-4) to 1.0 × 10(1) mg L(-1)	1033:1068	For determination of albumin, the fabricated MIP cryogel biosensor showed a high sensitivity with a wide linear range of 1.0 × 10(-4) to 1.0 × 10(1) mg L(-1) and a low limit of detection of 5.0 × 10(-5) mg L(-1) (S/N = 3).
25306849	2	1	theme	acrylamide	352:361	arg1	copolymerization					332:347	the graft copolymerization	322:347	the graft copolymerization of acrylamide with N,N'-methylenebisacrylamide on chitosan using human serum albumin (HSA) as the template	322:454	The MIP gel was prepared based on the graft copolymerization of acrylamide with N,N'-methylenebisacrylamide on chitosan using human serum albumin (HSA) as the template.
25306849	3	2	theme	macroporous	537:547	arg1	structure					557:565	the solvent to form ice crystals and left a macroporous cryogel structure	493:565	the solvent to form ice crystals and left a macroporous cryogel structure	493:565	The sub-zero polymerization allowed the solvent to form ice crystals and left a macroporous cryogel structure when it was thawed.
25306849	8	3	theme	bovine	1431:1436	arg1	BSA					1453:1455	BSA	1453:1455	BSA	1453:1455	The biosensor also showed a good selectivity, both against bovine serum albumin (BSA) and some common possible interfering compounds normally present in urine (ascorbic acid, uric acid, urea, sodium, chloride, potassium and creatinine).
25306849	8	3	theme	bovine	1431:1436	arg1	albumin					1444:1450	bovine serum albumin	1431:1450	bovine serum albumin (BSA)	1431:1456	The biosensor also showed a good selectivity, both against bovine serum albumin (BSA) and some common possible interfering compounds normally present in urine (ascorbic acid, uric acid, urea, sodium, chloride, potassium and creatinine).
25306849	6	4	theme	mg	1061:1062	arg1	-1					1066:1067	-1	1066:1067	-1	1066:1067	For determination of albumin, the fabricated MIP cryogel biosensor showed a high sensitivity with a wide linear range of 1.0 × 10(-4) to 1.0 × 10(1) mg L(-1) and a low limit of detection of 5.0 × 10(-5) mg L(-1) (S/N = 3).
25306849	6	4	theme	mg	1061:1062	arg1	L					1064:1064	1.0 × 10(-4) to 1.0 × 10(1) mg L	1033:1064	1.0 × 10(-4) to 1.0 × 10(1) mg L(-1)	1033:1068	For determination of albumin, the fabricated MIP cryogel biosensor showed a high sensitivity with a wide linear range of 1.0 × 10(-4) to 1.0 × 10(1) mg L(-1) and a low limit of detection of 5.0 × 10(-5) mg L(-1) (S/N = 3).
25306849	4	5	theme	cryogel	650:656	arg1	walls					663:667	cryogel pore walls	650:667	cryogel pore walls	650:667	After removing the template, the specific imprinted surface on cryogel pore walls was used to detect HSA via a redox mediator (ferrocene), entrapped in the cryogel, using differential pulse voltammetry (DPV).
25306849	6	6	theme	×	1106:1106	arg1	S/N					1125:1127	S/N = 3	1125:1131	S/N = 3	1125:1131	For determination of albumin, the fabricated MIP cryogel biosensor showed a high sensitivity with a wide linear range of 1.0 × 10(-4) to 1.0 × 10(1) mg L(-1) and a low limit of detection of 5.0 × 10(-5) mg L(-1) (S/N = 3).
25306849	6	6	theme	×	1106:1106	arg1	L					1118:1118	5.0 × 10(-5) mg L(-1)	1102:1122	5.0 × 10(-5) mg L(-1) (S/N = 3)	1102:1132	For determination of albumin, the fabricated MIP cryogel biosensor showed a high sensitivity with a wide linear range of 1.0 × 10(-4) to 1.0 × 10(1) mg L(-1) and a low limit of detection of 5.0 × 10(-5) mg L(-1) (S/N = 3).
25306849	3	7	theme	left	530:533	arg1	structure					557:565	the solvent to form ice crystals and left a macroporous cryogel structure	493:565	the solvent to form ice crystals and left a macroporous cryogel structure	493:565	The sub-zero polymerization allowed the solvent to form ice crystals and left a macroporous cryogel structure when it was thawed.
25306849	4	8	from	surface	639:645	arg1	walls					663:667	cryogel pore walls	650:667	cryogel pore walls	650:667	After removing the template, the specific imprinted surface on cryogel pore walls was used to detect HSA via a redox mediator (ferrocene), entrapped in the cryogel, using differential pulse voltammetry (DPV).
25306849	7	9	theme	good	1167:1170	arg1	reusability					1172:1182	a very good reusability	1160:1182	a very good reusability	1160:1182	The sensor also provided a very good reusability, i.e., the sensitivity remained >90% after 9 cycles of binding-rewashing (18 analyses per cycle), while the sensitivity only decreased to 90% after 6 weeks of storage at room temperature.
25306849	4	10	theme	specific	620:627	arg1	surface					639:645	the specific imprinted surface	616:645	the specific imprinted surface on cryogel pore walls	616:667	After removing the template, the specific imprinted surface on cryogel pore walls was used to detect HSA via a redox mediator (ferrocene), entrapped in the cryogel, using differential pulse voltammetry (DPV).
25306849	6	11	theme	=	1129:1129	arg1	S/N					1125:1127	S/N = 3	1125:1131	S/N = 3	1125:1131	For determination of albumin, the fabricated MIP cryogel biosensor showed a high sensitivity with a wide linear range of 1.0 × 10(-4) to 1.0 × 10(1) mg L(-1) and a low limit of detection of 5.0 × 10(-5) mg L(-1) (S/N = 3).
25306849	6	11	theme	=	1129:1129	arg1	L					1118:1118	5.0 × 10(-5) mg L(-1)	1102:1122	5.0 × 10(-5) mg L(-1) (S/N = 3)	1102:1132	For determination of albumin, the fabricated MIP cryogel biosensor showed a high sensitivity with a wide linear range of 1.0 × 10(-4) to 1.0 × 10(1) mg L(-1) and a low limit of detection of 5.0 × 10(-5) mg L(-1) (S/N = 3).
25306849	1	12	theme	cryogel	203:209	arg1	biosensor					211:219	A novel highly sensitive and selective molecularly imprinted polymer (MIP) cryogel biosensor	128:219	A novel highly sensitive and selective molecularly imprinted polymer (MIP) cryogel biosensor for determination of microalbumin in urine samples	128:270	A novel highly sensitive and selective molecularly imprinted polymer (MIP) cryogel biosensor for determination of microalbumin in urine samples was fabricated.
25306849	6	13	theme	detection	1089:1097	arg1	limit					1080:1084	a low limit	1074:1084	a low limit of detection of 5.0 × 10(-5) mg L(-1) (S/N = 3)	1074:1132	For determination of albumin, the fabricated MIP cryogel biosensor showed a high sensitivity with a wide linear range of 1.0 × 10(-4) to 1.0 × 10(1) mg L(-1) and a low limit of detection of 5.0 × 10(-5) mg L(-1) (S/N = 3).
25306849	6	13	theme	detection	1089:1097	arg1	sensitivity					993:1003	a high sensitivity	986:1003	a high sensitivity with a wide linear range of 1.0 × 10(-4) to 1.0 × 10(1) mg L(-1)	986:1068	For determination of albumin, the fabricated MIP cryogel biosensor showed a high sensitivity with a wide linear range of 1.0 × 10(-4) to 1.0 × 10(1) mg L(-1) and a low limit of detection of 5.0 × 10(-5) mg L(-1) (S/N = 3).
25306849	8	14	dep	urine	1525:1529	arg1	urea					1558:1561	urea	1558:1561	urea	1558:1561	The biosensor also showed a good selectivity, both against bovine serum albumin (BSA) and some common possible interfering compounds normally present in urine (ascorbic acid, uric acid, urea, sodium, chloride, potassium and creatinine).
25306849	8	14	dep	urine	1525:1529	arg1	acid					1541:1544	ascorbic acid	1532:1544	ascorbic acid	1532:1544	The biosensor also showed a good selectivity, both against bovine serum albumin (BSA) and some common possible interfering compounds normally present in urine (ascorbic acid, uric acid, urea, sodium, chloride, potassium and creatinine).
25306849	8	14	dep	urine	1525:1529	arg1	sodium					1564:1569	sodium	1564:1569	sodium	1564:1569	The biosensor also showed a good selectivity, both against bovine serum albumin (BSA) and some common possible interfering compounds normally present in urine (ascorbic acid, uric acid, urea, sodium, chloride, potassium and creatinine).
25306849	8	14	dep	urine	1525:1529	arg1	creatinine					1596:1605	creatinine	1596:1605	creatinine	1596:1605	The biosensor also showed a good selectivity, both against bovine serum albumin (BSA) and some common possible interfering compounds normally present in urine (ascorbic acid, uric acid, urea, sodium, chloride, potassium and creatinine).
25306849	8	14	dep	urine	1525:1529	arg1	acid					1552:1555	uric acid	1547:1555	uric acid	1547:1555	The biosensor also showed a good selectivity, both against bovine serum albumin (BSA) and some common possible interfering compounds normally present in urine (ascorbic acid, uric acid, urea, sodium, chloride, potassium and creatinine).
25306849	8	14	dep	urine	1525:1529	arg1	chloride					1572:1579	chloride	1572:1579	chloride	1572:1579	The biosensor also showed a good selectivity, both against bovine serum albumin (BSA) and some common possible interfering compounds normally present in urine (ascorbic acid, uric acid, urea, sodium, chloride, potassium and creatinine).
25306849	8	14	dep	urine	1525:1529	arg1	potassium					1582:1590	potassium	1582:1590	potassium	1582:1590	The biosensor also showed a good selectivity, both against bovine serum albumin (BSA) and some common possible interfering compounds normally present in urine (ascorbic acid, uric acid, urea, sodium, chloride, potassium and creatinine).
25306849	1	15	from	samples	264:270	arg1	determination					225:237	determination	225:237	determination of microalbumin in urine samples	225:270	A novel highly sensitive and selective molecularly imprinted polymer (MIP) cryogel biosensor for determination of microalbumin in urine samples was fabricated.
25306849	8	16	theme	common	1467:1472	arg1	compounds					1495:1503	some common possible interfering compounds	1462:1503	some common possible interfering compounds normally present in urine (ascorbic acid, uric acid, urea, sodium, chloride, potassium and creatinine)	1462:1606	The biosensor also showed a good selectivity, both against bovine serum albumin (BSA) and some common possible interfering compounds normally present in urine (ascorbic acid, uric acid, urea, sodium, chloride, potassium and creatinine).
25306849	5	17	theme	electrochemical	800:814	arg1	detection					816:824	The electrochemical detection	796:824	The electrochemical detection	796:824	The electrochemical detection was improved by the presence of graphene that has been composited within the polymer.
25306849	8	18	theme	interfering	1483:1493	arg1	compounds					1495:1503	some common possible interfering compounds	1462:1503	some common possible interfering compounds normally present in urine (ascorbic acid, uric acid, urea, sodium, chloride, potassium and creatinine)	1462:1606	The biosensor also showed a good selectivity, both against bovine serum albumin (BSA) and some common possible interfering compounds normally present in urine (ascorbic acid, uric acid, urea, sodium, chloride, potassium and creatinine).
25306849	4	19	theme	redox	698:702	arg1	ferrocene					714:722	ferrocene	714:722	ferrocene	714:722	After removing the template, the specific imprinted surface on cryogel pore walls was used to detect HSA via a redox mediator (ferrocene), entrapped in the cryogel, using differential pulse voltammetry (DPV).
25306849	4	19	theme	redox	698:702	arg1	mediator					704:711	a redox mediator	696:711	a redox mediator (ferrocene)	696:723	After removing the template, the specific imprinted surface on cryogel pore walls was used to detect HSA via a redox mediator (ferrocene), entrapped in the cryogel, using differential pulse voltammetry (DPV).
25306849	6	20	theme	L	1118:1118	arg1	detection					1089:1097	detection	1089:1097	detection of 5.0 × 10(-5) mg L(-1) (S/N = 3)	1089:1132	For determination of albumin, the fabricated MIP cryogel biosensor showed a high sensitivity with a wide linear range of 1.0 × 10(-4) to 1.0 × 10(1) mg L(-1) and a low limit of detection of 5.0 × 10(-5) mg L(-1) (S/N = 3).
25306849	0	21	used	used	99:102	arg2	biosensor					89:97	A novel molecularly imprinted chitosan-acrylamide, graphene, ferrocene composite cryogel biosensor	0:97	A novel molecularly imprinted chitosan-acrylamide, graphene, ferrocene composite cryogel biosensor	0:97	A novel molecularly imprinted chitosan-acrylamide, graphene, ferrocene composite cryogel biosensor used to detect microalbumin.
25306849	7	22	dep	remained	1207:1214	arg1	analyses					1261:1268	18 analyses	1258:1268	18 analyses per cycle	1258:1278	The sensor also provided a very good reusability, i.e., the sensitivity remained >90% after 9 cycles of binding-rewashing (18 analyses per cycle), while the sensitivity only decreased to 90% after 6 weeks of storage at room temperature.
25306849	1	23	theme	novel	130:134	arg1	biosensor					211:219	A novel highly sensitive and selective molecularly imprinted polymer (MIP) cryogel biosensor	128:219	A novel highly sensitive and selective molecularly imprinted polymer (MIP) cryogel biosensor for determination of microalbumin in urine samples	128:270	A novel highly sensitive and selective molecularly imprinted polymer (MIP) cryogel biosensor for determination of microalbumin in urine samples was fabricated.
25306849	6	24	theme	cryogel	961:967	arg1	biosensor					969:977	the fabricated MIP cryogel biosensor	942:977	the fabricated MIP cryogel biosensor	942:977	For determination of albumin, the fabricated MIP cryogel biosensor showed a high sensitivity with a wide linear range of 1.0 × 10(-4) to 1.0 × 10(1) mg L(-1) and a low limit of detection of 5.0 × 10(-5) mg L(-1) (S/N = 3).
25306849	9	25	theme	urine	1695:1699	arg1	samples					1701:1707	urine samples	1695:1707	urine samples	1695:1707	The excellent performance of the biosensor was confirmed by analyzing microalbumin in urine samples, and results were in good agreement with those obtained by the standard immunoturbidimetric method (P > 0.05).
25306849	0	26	theme	cryogel	81:87	arg1	biosensor					89:97	A novel molecularly imprinted chitosan-acrylamide, graphene, ferrocene composite cryogel biosensor	0:97	A novel molecularly imprinted chitosan-acrylamide, graphene, ferrocene composite cryogel biosensor	0:97	A novel molecularly imprinted chitosan-acrylamide, graphene, ferrocene composite cryogel biosensor used to detect microalbumin.
25306849	5	27	theme	graphene	858:865	arg1	presence					846:853	the presence	842:853	the presence of graphene that has been composited within the polymer	842:909	The electrochemical detection was improved by the presence of graphene that has been composited within the polymer.
25306849	6	28	theme	fabricated	946:955	arg1	biosensor					969:977	the fabricated MIP cryogel biosensor	942:977	the fabricated MIP cryogel biosensor	942:977	For determination of albumin, the fabricated MIP cryogel biosensor showed a high sensitivity with a wide linear range of 1.0 × 10(-4) to 1.0 × 10(1) mg L(-1) and a low limit of detection of 5.0 × 10(-5) mg L(-1) (S/N = 3).
25306849	2	29	theme	MIP	292:294	arg1	gel					296:298	The MIP gel	288:298	The MIP gel	288:298	The MIP gel was prepared based on the graft copolymerization of acrylamide with N,N'-methylenebisacrylamide on chitosan using human serum albumin (HSA) as the template.
25306849	7	30	from	temperature	1359:1369	arg1	storage					1343:1349	storage	1343:1349	storage at room temperature	1343:1369	The sensor also provided a very good reusability, i.e., the sensitivity remained >90% after 9 cycles of binding-rewashing (18 analyses per cycle), while the sensitivity only decreased to 90% after 6 weeks of storage at room temperature.
25306849	7	30	from	temperature	1359:1369	arg1	weeks					1334:1338	6 weeks	1332:1338	6 weeks of storage at room temperature	1332:1369	The sensor also provided a very good reusability, i.e., the sensitivity remained >90% after 9 cycles of binding-rewashing (18 analyses per cycle), while the sensitivity only decreased to 90% after 6 weeks of storage at room temperature.
25306849	8	31	theme	uric	1547:1550	arg1	acid					1541:1544	ascorbic acid	1532:1544	ascorbic acid	1532:1544	The biosensor also showed a good selectivity, both against bovine serum albumin (BSA) and some common possible interfering compounds normally present in urine (ascorbic acid, uric acid, urea, sodium, chloride, potassium and creatinine).
25306849	8	31	theme	uric	1547:1550	arg1	acid					1552:1555	uric acid	1547:1555	uric acid	1547:1555	The biosensor also showed a good selectivity, both against bovine serum albumin (BSA) and some common possible interfering compounds normally present in urine (ascorbic acid, uric acid, urea, sodium, chloride, potassium and creatinine).
25306849	6	32	theme	wide	1012:1015	arg1	range					1024:1028	a wide linear range	1010:1028	a wide linear range of 1.0 × 10(-4) to 1.0 × 10(1) mg L(-1)	1010:1068	For determination of albumin, the fabricated MIP cryogel biosensor showed a high sensitivity with a wide linear range of 1.0 × 10(-4) to 1.0 × 10(1) mg L(-1) and a low limit of detection of 5.0 × 10(-5) mg L(-1) (S/N = 3).
25306849	1	33	dep	sensitive	143:151	arg1	MIP					198:200	MIP	198:200	MIP	198:200	A novel highly sensitive and selective molecularly imprinted polymer (MIP) cryogel biosensor for determination of microalbumin in urine samples was fabricated.
25306849	1	33	dep	sensitive	143:151	arg1	polymer					189:195	molecularly imprinted polymer	167:195	molecularly imprinted polymer (MIP)	167:201	A novel highly sensitive and selective molecularly imprinted polymer (MIP) cryogel biosensor for determination of microalbumin in urine samples was fabricated.
25306849	6	34	dep	×	1037:1037	arg1	×					1053:1053	1.0 × 10(1)	1049:1059	1.0 × 10(-4) to 1.0 × 10(1) mg L(-1)	1033:1068	For determination of albumin, the fabricated MIP cryogel biosensor showed a high sensitivity with a wide linear range of 1.0 × 10(-4) to 1.0 × 10(1) mg L(-1) and a low limit of detection of 5.0 × 10(-5) mg L(-1) (S/N = 3).
25306849	6	34	dep	×	1037:1037	arg1	to					1046:1047	to	1046:1047	to	1046:1047	For determination of albumin, the fabricated MIP cryogel biosensor showed a high sensitivity with a wide linear range of 1.0 × 10(-4) to 1.0 × 10(1) mg L(-1) and a low limit of detection of 5.0 × 10(-5) mg L(-1) (S/N = 3).
25306849	2	35	theme	serum	420:424	arg1	HSA					435:437	HSA	435:437	HSA	435:437	The MIP gel was prepared based on the graft copolymerization of acrylamide with N,N'-methylenebisacrylamide on chitosan using human serum albumin (HSA) as the template.
25306849	2	35	theme	serum	420:424	arg1	albumin					426:432	human serum albumin	414:432	human serum albumin (HSA)	414:438	The MIP gel was prepared based on the graft copolymerization of acrylamide with N,N'-methylenebisacrylamide on chitosan using human serum albumin (HSA) as the template.
25306849	2	35	theme	serum	420:424	arg1	template					447:454	the template	443:454	the template	443:454	The MIP gel was prepared based on the graft copolymerization of acrylamide with N,N'-methylenebisacrylamide on chitosan using human serum albumin (HSA) as the template.
25306849	0	36	theme	chitosan-acrylamide	30:48	arg1	biosensor					89:97	A novel molecularly imprinted chitosan-acrylamide, graphene, ferrocene composite cryogel biosensor	0:97	A novel molecularly imprinted chitosan-acrylamide, graphene, ferrocene composite cryogel biosensor	0:97	A novel molecularly imprinted chitosan-acrylamide, graphene, ferrocene composite cryogel biosensor used to detect microalbumin.
25306849	6	37	theme	high	988:991	arg1	sensitivity					993:1003	a high sensitivity	986:1003	a high sensitivity with a wide linear range of 1.0 × 10(-4) to 1.0 × 10(1) mg L(-1)	986:1068	For determination of albumin, the fabricated MIP cryogel biosensor showed a high sensitivity with a wide linear range of 1.0 × 10(-4) to 1.0 × 10(1) mg L(-1) and a low limit of detection of 5.0 × 10(-5) mg L(-1) (S/N = 3).
25306849	6	38	with	sensitivity	993:1003	arg1	range					1024:1028	a wide linear range	1010:1028	a wide linear range of 1.0 × 10(-4) to 1.0 × 10(1) mg L(-1)	1010:1068	For determination of albumin, the fabricated MIP cryogel biosensor showed a high sensitivity with a wide linear range of 1.0 × 10(-4) to 1.0 × 10(1) mg L(-1) and a low limit of detection of 5.0 × 10(-5) mg L(-1) (S/N = 3).
25306849	9	39	theme	standard	1772:1779	arg1	>					1811:1811	P > 0.05	1809:1816	P > 0.05	1809:1816	The excellent performance of the biosensor was confirmed by analyzing microalbumin in urine samples, and results were in good agreement with those obtained by the standard immunoturbidimetric method (P > 0.05).
25306849	9	39	theme	standard	1772:1779	arg1	method					1801:1806	the standard immunoturbidimetric method	1768:1806	the standard immunoturbidimetric method (P > 0.05)	1768:1817	The excellent performance of the biosensor was confirmed by analyzing microalbumin in urine samples, and results were in good agreement with those obtained by the standard immunoturbidimetric method (P > 0.05).
25306849	2	40	theme	graft	326:330	arg1	copolymerization					332:347	the graft copolymerization	322:347	the graft copolymerization of acrylamide with N,N'-methylenebisacrylamide on chitosan using human serum albumin (HSA) as the template	322:454	The MIP gel was prepared based on the graft copolymerization of acrylamide with N,N'-methylenebisacrylamide on chitosan using human serum albumin (HSA) as the template.
25306849	4	41	theme	differential	758:769	arg1	voltammetry					777:787	differential pulse voltammetry	758:787	differential pulse voltammetry (DPV)	758:793	After removing the template, the specific imprinted surface on cryogel pore walls was used to detect HSA via a redox mediator (ferrocene), entrapped in the cryogel, using differential pulse voltammetry (DPV).
25306849	4	41	theme	differential	758:769	arg1	DPV					790:792	DPV	790:792	DPV	790:792	After removing the template, the specific imprinted surface on cryogel pore walls was used to detect HSA via a redox mediator (ferrocene), entrapped in the cryogel, using differential pulse voltammetry (DPV).
25306849	3	42	theme	ice	513:515	arg1	crystals					517:524	ice crystals	513:524	ice crystals	513:524	The sub-zero polymerization allowed the solvent to form ice crystals and left a macroporous cryogel structure when it was thawed.
25306849	1	43	theme	microalbumin	242:253	arg1	determination					225:237	determination	225:237	determination of microalbumin in urine samples	225:270	A novel highly sensitive and selective molecularly imprinted polymer (MIP) cryogel biosensor for determination of microalbumin in urine samples was fabricated.
25306849	7	44	theme	binding-rewashing	1239:1255	arg1	cycles					1229:1234	9 cycles	1227:1234	9 cycles of binding-rewashing	1227:1255	The sensor also provided a very good reusability, i.e., the sensitivity remained >90% after 9 cycles of binding-rewashing (18 analyses per cycle), while the sensitivity only decreased to 90% after 6 weeks of storage at room temperature.
25306849	9	45	from	microalbumin	1679:1690	arg1	samples					1701:1707	urine samples	1695:1707	urine samples	1695:1707	The excellent performance of the biosensor was confirmed by analyzing microalbumin in urine samples, and results were in good agreement with those obtained by the standard immunoturbidimetric method (P > 0.05).
25306849	9	46	theme	P	1809:1809	arg1	>					1811:1811	P > 0.05	1809:1816	P > 0.05	1809:1816	The excellent performance of the biosensor was confirmed by analyzing microalbumin in urine samples, and results were in good agreement with those obtained by the standard immunoturbidimetric method (P > 0.05).
25306849	9	46	theme	P	1809:1809	arg1	method					1801:1806	the standard immunoturbidimetric method	1768:1806	the standard immunoturbidimetric method (P > 0.05)	1768:1817	The excellent performance of the biosensor was confirmed by analyzing microalbumin in urine samples, and results were in good agreement with those obtained by the standard immunoturbidimetric method (P > 0.05).
25306849	6	47	theme	mg	1115:1116	arg1	S/N					1125:1127	S/N = 3	1125:1131	S/N = 3	1125:1131	For determination of albumin, the fabricated MIP cryogel biosensor showed a high sensitivity with a wide linear range of 1.0 × 10(-4) to 1.0 × 10(1) mg L(-1) and a low limit of detection of 5.0 × 10(-5) mg L(-1) (S/N = 3).
25306849	6	47	theme	mg	1115:1116	arg1	L					1118:1118	5.0 × 10(-5) mg L(-1)	1102:1122	5.0 × 10(-5) mg L(-1) (S/N = 3)	1102:1132	For determination of albumin, the fabricated MIP cryogel biosensor showed a high sensitivity with a wide linear range of 1.0 × 10(-4) to 1.0 × 10(1) mg L(-1) and a low limit of detection of 5.0 × 10(-5) mg L(-1) (S/N = 3).
25306849	8	48	theme	good	1400:1403	arg1	selectivity					1405:1415	a good selectivity	1398:1415	a good selectivity	1398:1415	The biosensor also showed a good selectivity, both against bovine serum albumin (BSA) and some common possible interfering compounds normally present in urine (ascorbic acid, uric acid, urea, sodium, chloride, potassium and creatinine).
25306849	1	49	theme	urine	258:262	arg1	samples					264:270	urine samples	258:270	urine samples	258:270	A novel highly sensitive and selective molecularly imprinted polymer (MIP) cryogel biosensor for determination of microalbumin in urine samples was fabricated.
25306849	3	50	theme	a	535:535	arg1	structure					557:565	the solvent to form ice crystals and left a macroporous cryogel structure	493:565	the solvent to form ice crystals and left a macroporous cryogel structure	493:565	The sub-zero polymerization allowed the solvent to form ice crystals and left a macroporous cryogel structure when it was thawed.
25306849	6	51	theme	L	1064:1064	arg1	range					1024:1028	a wide linear range	1010:1028	a wide linear range of 1.0 × 10(-4) to 1.0 × 10(1) mg L(-1)	1010:1068	For determination of albumin, the fabricated MIP cryogel biosensor showed a high sensitivity with a wide linear range of 1.0 × 10(-4) to 1.0 × 10(1) mg L(-1) and a low limit of detection of 5.0 × 10(-5) mg L(-1) (S/N = 3).
25306849	1	52	theme	imprinted	179:187	arg1	MIP					198:200	MIP	198:200	MIP	198:200	A novel highly sensitive and selective molecularly imprinted polymer (MIP) cryogel biosensor for determination of microalbumin in urine samples was fabricated.
25306849	1	52	theme	imprinted	179:187	arg1	polymer					189:195	molecularly imprinted polymer	167:195	molecularly imprinted polymer (MIP)	167:201	A novel highly sensitive and selective molecularly imprinted polymer (MIP) cryogel biosensor for determination of microalbumin in urine samples was fabricated.
25306849	8	53	theme	serum	1438:1442	arg1	BSA					1453:1455	BSA	1453:1455	BSA	1453:1455	The biosensor also showed a good selectivity, both against bovine serum albumin (BSA) and some common possible interfering compounds normally present in urine (ascorbic acid, uric acid, urea, sodium, chloride, potassium and creatinine).
25306849	8	53	theme	serum	1438:1442	arg1	albumin					1444:1450	bovine serum albumin	1431:1450	bovine serum albumin (BSA)	1431:1456	The biosensor also showed a good selectivity, both against bovine serum albumin (BSA) and some common possible interfering compounds normally present in urine (ascorbic acid, uric acid, urea, sodium, chloride, potassium and creatinine).
25306849	6	54	theme	albumin	933:939	arg1	determination					916:928	determination	916:928	determination of albumin	916:939	For determination of albumin, the fabricated MIP cryogel biosensor showed a high sensitivity with a wide linear range of 1.0 × 10(-4) to 1.0 × 10(1) mg L(-1) and a low limit of detection of 5.0 × 10(-5) mg L(-1) (S/N = 3).
25306849	9	55	theme	excellent	1613:1621	arg1	performance					1623:1633	The excellent performance	1609:1633	The excellent performance of the biosensor	1609:1650	The excellent performance of the biosensor was confirmed by analyzing microalbumin in urine samples, and results were in good agreement with those obtained by the standard immunoturbidimetric method (P > 0.05).
25306849	4	56	theme	pore	658:661	arg1	walls					663:667	cryogel pore walls	650:667	cryogel pore walls	650:667	After removing the template, the specific imprinted surface on cryogel pore walls was used to detect HSA via a redox mediator (ferrocene), entrapped in the cryogel, using differential pulse voltammetry (DPV).
25306849	9	57	with	agreement	1735:1743	arg1	those					1750:1754	those	1750:1754	those	1750:1754	The excellent performance of the biosensor was confirmed by analyzing microalbumin in urine samples, and results were in good agreement with those obtained by the standard immunoturbidimetric method (P > 0.05).
25306849	3	58	theme	cryogel	549:555	arg1	structure					557:565	the solvent to form ice crystals and left a macroporous cryogel structure	493:565	the solvent to form ice crystals and left a macroporous cryogel structure	493:565	The sub-zero polymerization allowed the solvent to form ice crystals and left a macroporous cryogel structure when it was thawed.
25306849	7	59	theme	room	1354:1357	arg1	temperature					1359:1369	room temperature	1354:1369	room temperature	1354:1369	The sensor also provided a very good reusability, i.e., the sensitivity remained >90% after 9 cycles of binding-rewashing (18 analyses per cycle), while the sensitivity only decreased to 90% after 6 weeks of storage at room temperature.
25306849	8	60	from	present	1514:1520	arg1	urine					1525:1529	urine	1525:1529	urine (ascorbic acid, uric acid, urea, sodium, chloride, potassium and creatinine)	1525:1606	The biosensor also showed a good selectivity, both against bovine serum albumin (BSA) and some common possible interfering compounds normally present in urine (ascorbic acid, uric acid, urea, sodium, chloride, potassium and creatinine).
25306849	7	61	theme	storage	1343:1349	arg1	weeks					1334:1338	6 weeks	1332:1338	6 weeks of storage at room temperature	1332:1369	The sensor also provided a very good reusability, i.e., the sensitivity remained >90% after 9 cycles of binding-rewashing (18 analyses per cycle), while the sensitivity only decreased to 90% after 6 weeks of storage at room temperature.
25306849	9	62	theme	biosensor	1642:1650	arg1	performance					1623:1633	The excellent performance	1609:1633	The excellent performance of the biosensor	1609:1650	The excellent performance of the biosensor was confirmed by analyzing microalbumin in urine samples, and results were in good agreement with those obtained by the standard immunoturbidimetric method (P > 0.05).
25306849	4	63	theme	imprinted	629:637	arg1	surface					639:645	the specific imprinted surface	616:645	the specific imprinted surface on cryogel pore walls	616:667	After removing the template, the specific imprinted surface on cryogel pore walls was used to detect HSA via a redox mediator (ferrocene), entrapped in the cryogel, using differential pulse voltammetry (DPV).
25306849	1	64	from	microalbumin	242:253	arg1	samples					264:270	urine samples	258:270	urine samples	258:270	A novel highly sensitive and selective molecularly imprinted polymer (MIP) cryogel biosensor for determination of microalbumin in urine samples was fabricated.
25306849	6	65	theme	low	1076:1078	arg1	limit					1080:1084	a low limit	1074:1084	a low limit of detection of 5.0 × 10(-5) mg L(-1) (S/N = 3)	1074:1132	For determination of albumin, the fabricated MIP cryogel biosensor showed a high sensitivity with a wide linear range of 1.0 × 10(-4) to 1.0 × 10(1) mg L(-1) and a low limit of detection of 5.0 × 10(-5) mg L(-1) (S/N = 3).
25306849	1	66	from	determination	225:237	arg1	samples					264:270	urine samples	258:270	urine samples	258:270	A novel highly sensitive and selective molecularly imprinted polymer (MIP) cryogel biosensor for determination of microalbumin in urine samples was fabricated.
25306849	8	67	theme	possible	1474:1481	arg1	compounds					1495:1503	some common possible interfering compounds	1462:1503	some common possible interfering compounds normally present in urine (ascorbic acid, uric acid, urea, sodium, chloride, potassium and creatinine)	1462:1606	The biosensor also showed a good selectivity, both against bovine serum albumin (BSA) and some common possible interfering compounds normally present in urine (ascorbic acid, uric acid, urea, sodium, chloride, potassium and creatinine).
25306849	7	68	dep	sensitivity	1195:1205	arg1	i.e.					1185:1188	i.e.	1185:1188	i.e.	1185:1188	The sensor also provided a very good reusability, i.e., the sensitivity remained >90% after 9 cycles of binding-rewashing (18 analyses per cycle), while the sensitivity only decreased to 90% after 6 weeks of storage at room temperature.
25306849	6	69	with	limit	1080:1084	arg1	range					1024:1028	a wide linear range	1010:1028	a wide linear range of 1.0 × 10(-4) to 1.0 × 10(1) mg L(-1)	1010:1068	For determination of albumin, the fabricated MIP cryogel biosensor showed a high sensitivity with a wide linear range of 1.0 × 10(-4) to 1.0 × 10(1) mg L(-1) and a low limit of detection of 5.0 × 10(-5) mg L(-1) (S/N = 3).
25306849	8	70	from	urine	1525:1529	arg1	present					1514:1520	present	1514:1520	present	1514:1520	The biosensor also showed a good selectivity, both against bovine serum albumin (BSA) and some common possible interfering compounds normally present in urine (ascorbic acid, uric acid, urea, sodium, chloride, potassium and creatinine).
25306849	0	71	theme	composite	71:79	arg1	biosensor					89:97	A novel molecularly imprinted chitosan-acrylamide, graphene, ferrocene composite cryogel biosensor	0:97	A novel molecularly imprinted chitosan-acrylamide, graphene, ferrocene composite cryogel biosensor	0:97	A novel molecularly imprinted chitosan-acrylamide, graphene, ferrocene composite cryogel biosensor used to detect microalbumin.
25306849	1	72	theme	sensitive	143:151	arg1	biosensor					211:219	A novel highly sensitive and selective molecularly imprinted polymer (MIP) cryogel biosensor	128:219	A novel highly sensitive and selective molecularly imprinted polymer (MIP) cryogel biosensor for determination of microalbumin in urine samples	128:270	A novel highly sensitive and selective molecularly imprinted polymer (MIP) cryogel biosensor for determination of microalbumin in urine samples was fabricated.
25306849	7	73	from	weeks	1334:1338	arg1	temperature					1359:1369	room temperature	1354:1369	room temperature	1354:1369	The sensor also provided a very good reusability, i.e., the sensitivity remained >90% after 9 cycles of binding-rewashing (18 analyses per cycle), while the sensitivity only decreased to 90% after 6 weeks of storage at room temperature.
25306849	8	74	theme	present	1514:1520	arg1	compounds					1495:1503	some common possible interfering compounds	1462:1503	some common possible interfering compounds normally present in urine (ascorbic acid, uric acid, urea, sodium, chloride, potassium and creatinine)	1462:1606	The biosensor also showed a good selectivity, both against bovine serum albumin (BSA) and some common possible interfering compounds normally present in urine (ascorbic acid, uric acid, urea, sodium, chloride, potassium and creatinine).
25306849	1	75	theme	selective	157:165	arg1	biosensor					211:219	A novel highly sensitive and selective molecularly imprinted polymer (MIP) cryogel biosensor	128:219	A novel highly sensitive and selective molecularly imprinted polymer (MIP) cryogel biosensor for determination of microalbumin in urine samples	128:270	A novel highly sensitive and selective molecularly imprinted polymer (MIP) cryogel biosensor for determination of microalbumin in urine samples was fabricated.
25306849	6	76	theme	MIP	957:959	arg1	biosensor					969:977	the fabricated MIP cryogel biosensor	942:977	the fabricated MIP cryogel biosensor	942:977	For determination of albumin, the fabricated MIP cryogel biosensor showed a high sensitivity with a wide linear range of 1.0 × 10(-4) to 1.0 × 10(1) mg L(-1) and a low limit of detection of 5.0 × 10(-5) mg L(-1) (S/N = 3).
25306849	8	77	theme	ascorbic	1532:1539	arg1	urea					1558:1561	urea	1558:1561	urea	1558:1561	The biosensor also showed a good selectivity, both against bovine serum albumin (BSA) and some common possible interfering compounds normally present in urine (ascorbic acid, uric acid, urea, sodium, chloride, potassium and creatinine).
25306849	8	77	theme	ascorbic	1532:1539	arg1	acid					1541:1544	ascorbic acid	1532:1544	ascorbic acid	1532:1544	The biosensor also showed a good selectivity, both against bovine serum albumin (BSA) and some common possible interfering compounds normally present in urine (ascorbic acid, uric acid, urea, sodium, chloride, potassium and creatinine).
25306849	8	77	theme	ascorbic	1532:1539	arg1	sodium					1564:1569	sodium	1564:1569	sodium	1564:1569	The biosensor also showed a good selectivity, both against bovine serum albumin (BSA) and some common possible interfering compounds normally present in urine (ascorbic acid, uric acid, urea, sodium, chloride, potassium and creatinine).
25306849	8	77	theme	ascorbic	1532:1539	arg1	creatinine					1596:1605	creatinine	1596:1605	creatinine	1596:1605	The biosensor also showed a good selectivity, both against bovine serum albumin (BSA) and some common possible interfering compounds normally present in urine (ascorbic acid, uric acid, urea, sodium, chloride, potassium and creatinine).
25306849	8	77	theme	ascorbic	1532:1539	arg1	acid					1552:1555	uric acid	1547:1555	uric acid	1547:1555	The biosensor also showed a good selectivity, both against bovine serum albumin (BSA) and some common possible interfering compounds normally present in urine (ascorbic acid, uric acid, urea, sodium, chloride, potassium and creatinine).
25306849	8	77	theme	ascorbic	1532:1539	arg1	chloride					1572:1579	chloride	1572:1579	chloride	1572:1579	The biosensor also showed a good selectivity, both against bovine serum albumin (BSA) and some common possible interfering compounds normally present in urine (ascorbic acid, uric acid, urea, sodium, chloride, potassium and creatinine).
25306849	8	77	theme	ascorbic	1532:1539	arg1	potassium					1582:1590	potassium	1582:1590	potassium	1582:1590	The biosensor also showed a good selectivity, both against bovine serum albumin (BSA) and some common possible interfering compounds normally present in urine (ascorbic acid, uric acid, urea, sodium, chloride, potassium and creatinine).
25306849	8	78	dep	albumin	1444:1450	arg1	both					1418:1421	both	1418:1421	both	1418:1421	The biosensor also showed a good selectivity, both against bovine serum albumin (BSA) and some common possible interfering compounds normally present in urine (ascorbic acid, uric acid, urea, sodium, chloride, potassium and creatinine).
25306849	0	79	theme	novel	2:6	arg1	biosensor					89:97	A novel molecularly imprinted chitosan-acrylamide, graphene, ferrocene composite cryogel biosensor	0:97	A novel molecularly imprinted chitosan-acrylamide, graphene, ferrocene composite cryogel biosensor	0:97	A novel molecularly imprinted chitosan-acrylamide, graphene, ferrocene composite cryogel biosensor used to detect microalbumin.
25306849	6	80	theme	linear	1017:1022	arg1	range					1024:1028	a wide linear range	1010:1028	a wide linear range of 1.0 × 10(-4) to 1.0 × 10(1) mg L(-1)	1010:1068	For determination of albumin, the fabricated MIP cryogel biosensor showed a high sensitivity with a wide linear range of 1.0 × 10(-4) to 1.0 × 10(1) mg L(-1) and a low limit of detection of 5.0 × 10(-5) mg L(-1) (S/N = 3).
25306849	9	81	theme	good	1730:1733	arg1	agreement					1735:1743	good agreement	1730:1743	good agreement with those obtained by the standard immunoturbidimetric method (P > 0.05)	1730:1817	The excellent performance of the biosensor was confirmed by analyzing microalbumin in urine samples, and results were in good agreement with those obtained by the standard immunoturbidimetric method (P > 0.05).
25306849	0	82	theme	imprinted	20:28	arg1	ferrocene					61:69	ferrocene	61:69	ferrocene	61:69	A novel molecularly imprinted chitosan-acrylamide, graphene, ferrocene composite cryogel biosensor used to detect microalbumin.
25306849	0	82	theme	imprinted	20:28	arg1	graphene					51:58	graphene	51:58	graphene	51:58	A novel molecularly imprinted chitosan-acrylamide, graphene, ferrocene composite cryogel biosensor used to detect microalbumin.
25306849	0	82	theme	imprinted	20:28	arg1	chitosan-acrylamide					30:48	molecularly imprinted chitosan-acrylamide	8:48	molecularly imprinted chitosan-acrylamide	8:48	A novel molecularly imprinted chitosan-acrylamide, graphene, ferrocene composite cryogel biosensor used to detect microalbumin.
25306849	4	83	used	used	673:676	arg2	walls					663:667	cryogel pore walls	650:667	cryogel pore walls	650:667	After removing the template, the specific imprinted surface on cryogel pore walls was used to detect HSA via a redox mediator (ferrocene), entrapped in the cryogel, using differential pulse voltammetry (DPV).
25306849	4	83	used	used	673:676	arg2	surface					639:645	the specific imprinted surface	616:645	the specific imprinted surface on cryogel pore walls	616:667	After removing the template, the specific imprinted surface on cryogel pore walls was used to detect HSA via a redox mediator (ferrocene), entrapped in the cryogel, using differential pulse voltammetry (DPV).
25306849	3	84	theme	sub-zero	461:468	arg1	polymerization					470:483	The sub-zero polymerization	457:483	The sub-zero polymerization	457:483	The sub-zero polymerization allowed the solvent to form ice crystals and left a macroporous cryogel structure when it was thawed.
25306849	4	85	theme	pulse	771:775	arg1	voltammetry					777:787	differential pulse voltammetry	758:787	differential pulse voltammetry (DPV)	758:793	After removing the template, the specific imprinted surface on cryogel pore walls was used to detect HSA via a redox mediator (ferrocene), entrapped in the cryogel, using differential pulse voltammetry (DPV).
25306849	4	85	theme	pulse	771:775	arg1	DPV					790:792	DPV	790:792	DPV	790:792	After removing the template, the specific imprinted surface on cryogel pore walls was used to detect HSA via a redox mediator (ferrocene), entrapped in the cryogel, using differential pulse voltammetry (DPV).
25306849	2	86	theme	human	414:418	arg1	HSA					435:437	HSA	435:437	HSA	435:437	The MIP gel was prepared based on the graft copolymerization of acrylamide with N,N'-methylenebisacrylamide on chitosan using human serum albumin (HSA) as the template.
25306849	2	86	theme	human	414:418	arg1	albumin					426:432	human serum albumin	414:432	human serum albumin (HSA)	414:438	The MIP gel was prepared based on the graft copolymerization of acrylamide with N,N'-methylenebisacrylamide on chitosan using human serum albumin (HSA) as the template.
25306849	2	86	theme	human	414:418	arg1	template					447:454	the template	443:454	the template	443:454	The MIP gel was prepared based on the graft copolymerization of acrylamide with N,N'-methylenebisacrylamide on chitosan using human serum albumin (HSA) as the template.
25306849	2	87	with	copolymerization	332:347	arg1	N'-methylenebisacrylamide					370:394	N'-methylenebisacrylamide	370:394	N'-methylenebisacrylamide on chitosan using human serum albumin (HSA) as the template	370:454	The MIP gel was prepared based on the graft copolymerization of acrylamide with N,N'-methylenebisacrylamide on chitosan using human serum albumin (HSA) as the template.
25306849	2	87	with	copolymerization	332:347	arg1	N					368:368	N	368:368	N	368:368	The MIP gel was prepared based on the graft copolymerization of acrylamide with N,N'-methylenebisacrylamide on chitosan using human serum albumin (HSA) as the template.
25306849	2	88	from	N'-methylenebisacrylamide	370:394	arg1	chitosan					399:406	chitosan	399:406	chitosan using human serum albumin (HSA) as the template	399:454	The MIP gel was prepared based on the graft copolymerization of acrylamide with N,N'-methylenebisacrylamide on chitosan using human serum albumin (HSA) as the template.
25306849	8	89	attach	present	1514:1520	arg1	urine					1525:1529	urine	1525:1529	urine (ascorbic acid, uric acid, urea, sodium, chloride, potassium and creatinine)	1525:1606	The biosensor also showed a good selectivity, both against bovine serum albumin (BSA) and some common possible interfering compounds normally present in urine (ascorbic acid, uric acid, urea, sodium, chloride, potassium and creatinine).
25306849	8	89	attach	present	1514:1520	arg2	compounds					1495:1503	some common possible interfering compounds	1462:1503	some common possible interfering compounds normally present in urine (ascorbic acid, uric acid, urea, sodium, chloride, potassium and creatinine)	1462:1606	The biosensor also showed a good selectivity, both against bovine serum albumin (BSA) and some common possible interfering compounds normally present in urine (ascorbic acid, uric acid, urea, sodium, chloride, potassium and creatinine).
25306849	3	90	theme	solvent	497:503	arg1	structure					557:565	the solvent to form ice crystals and left a macroporous cryogel structure	493:565	the solvent to form ice crystals and left a macroporous cryogel structure	493:565	The sub-zero polymerization allowed the solvent to form ice crystals and left a macroporous cryogel structure when it was thawed.
25306849	9	91	theme	immunoturbidimetric	1781:1799	arg1	>					1811:1811	P > 0.05	1809:1816	P > 0.05	1809:1816	The excellent performance of the biosensor was confirmed by analyzing microalbumin in urine samples, and results were in good agreement with those obtained by the standard immunoturbidimetric method (P > 0.05).
25306849	9	91	theme	immunoturbidimetric	1781:1799	arg1	method					1801:1806	the standard immunoturbidimetric method	1768:1806	the standard immunoturbidimetric method (P > 0.05)	1768:1817	The excellent performance of the biosensor was confirmed by analyzing microalbumin in urine samples, and results were in good agreement with those obtained by the standard immunoturbidimetric method (P > 0.05).
27109145	6	0	theme	vapour	961:966	arg1	rates					981:985	water vapour transmission rates	955:985	water vapour transmission rates comparable to commercial wound dressings	955:1026	The crosslinked sponges also demonstrated water vapour transmission rates comparable to commercial wound dressings.
27109145	4	1	theme	maximum	647:653	arg1	index					668:672	a maximum crosslinking index	645:672	a maximum crosslinking index of 82.1 ± 1.3%	645:687	Fourier transform infrared spectroscopy (FTIR) and free amine group quantification using ninhydrin revealed a maximum crosslinking index of 82.1 ± 1.3%.
27109145	11	2	theme	relevant	2025:2032	arg1	exploitations					2034:2046	clinically relevant exploitations	2014:2046	clinically relevant exploitations	2014:2046	These findings demonstrated the feasibility of producing crosslinked human hair keratin-alginate sponges, with tuneable physical and mechanical properties, which are cell compliant in vitro and biocompatible in vivo, suggesting their potential for clinically relevant exploitations.
27109145	2	3	theme	practical	297:305	arg1	relevance					307:315	their practical relevance	291:315	their practical relevance	291:315	However, pure keratin templates have poor mechanical properties, which limit their practical relevance.
27109145	9	4	theme	sponges	1526:1532	arg1	implantation					1489:1500	subcutaneous implantation	1476:1500	subcutaneous implantation of the keratin-alginate sponges into C57BL/6NTac mice over 4 weeks	1476:1567	In addition, subcutaneous implantation of the keratin-alginate sponges into C57BL/6NTac mice over 4 weeks showed no significant immunological reaction and minimal formation of fibrotic capsules.
27109145	9	5	theme	C57BL/6NTac	1539:1549	arg1	mice					1551:1554	C57BL/6NTac mice	1539:1554	C57BL/6NTac mice over 4 weeks	1539:1567	In addition, subcutaneous implantation of the keratin-alginate sponges into C57BL/6NTac mice over 4 weeks showed no significant immunological reaction and minimal formation of fibrotic capsules.
27109145	8	6	theme	keratin	1252:1258	arg1	proportions					1237:1247	Higher proportions	1230:1247	Higher proportions of keratin on coated two-dimensional surfaces and in three-dimensional sponges	1230:1326	Higher proportions of keratin on coated two-dimensional surfaces and in three-dimensional sponges resulted in more attachment and improved proliferation of L929 fibroblasts, verifying the bioactive role of keratin in the composites.
27109145	0	7	link	crosslinked	44:54	arg1	sponge					79:84	a novel crosslinked human keratin-alginate sponge	36:84	a novel crosslinked human keratin-alginate sponge	36:84	Fabrication and characterization of a novel crosslinked human keratin-alginate sponge.
27109145	10	8	theme	neo-tissue	1716:1725	arg1	formation					1727:1735	neo-tissue formation	1716:1735	neo-tissue formation	1716:1735	Furthermore, the sponges supported cellular infiltration, neo-tissue formation and vascularization in vivo.
27109145	7	9	theme	proteinase	1152:1161	arg1	K					1163:1163	proteinase K	1152:1163	proteinase K	1152:1163	Meanwhile, sponges with higher proportions of keratin showed lower water uptake capacities and higher degradation rates by proteinase K, in comparison with sponges with higher proportions of alginate.
27109145	8	10	theme	coated	1263:1268	arg1	surfaces					1286:1293	coated two-dimensional surfaces	1263:1293	coated two-dimensional surfaces	1263:1293	Higher proportions of keratin on coated two-dimensional surfaces and in three-dimensional sponges resulted in more attachment and improved proliferation of L929 fibroblasts, verifying the bioactive role of keratin in the composites.
27109145	6	11	theme	wound	1012:1016	arg1	dressings					1018:1026	commercial wound dressings	1001:1026	commercial wound dressings	1001:1026	The crosslinked sponges also demonstrated water vapour transmission rates comparable to commercial wound dressings.
27109145	3	12	with	sponge	357:362	arg1	properties					525:534	improved mechanical properties	505:534	improved mechanical properties	505:534	Herein, we described a novel composite sponge, consisting of human hair keratins chemically crosslinked with alginate using 1-ethyl-3-dimethylaminopropyl carbodiimide hydrochloride, with improved mechanical properties.
27109145	7	13	theme	higher	1124:1129	arg1	rates					1143:1147	higher degradation rates	1124:1147	higher degradation rates	1124:1147	Meanwhile, sponges with higher proportions of keratin showed lower water uptake capacities and higher degradation rates by proteinase K, in comparison with sponges with higher proportions of alginate.
27109145	7	14	theme	keratin	1075:1081	arg1	proportions					1060:1070	higher proportions	1053:1070	higher proportions of keratin	1053:1081	Meanwhile, sponges with higher proportions of keratin showed lower water uptake capacities and higher degradation rates by proteinase K, in comparison with sponges with higher proportions of alginate.
27109145	5	15	theme	increased	750:758	arg1	strength					768:775	increased tensile strength	750:775	increased tensile strength	750:775	With increasing alginate proportions, the sponges exhibited increased tensile strength, tensile modulus and compression modulus at maximum values of 10.3 ± 1.92 kPa, 219.07 ± 52.39 kPa and 191.48 ± 32.89 kPa, respectively.
27109145	2	16	theme	keratin	228:234	arg1	templates					236:244	pure keratin templates	223:244	pure keratin templates	223:244	However, pure keratin templates have poor mechanical properties, which limit their practical relevance.
27109145	9	17	theme	immunological	1591:1603	arg1	reaction					1605:1612	no significant immunological reaction	1576:1612	no significant immunological reaction	1576:1612	In addition, subcutaneous implantation of the keratin-alginate sponges into C57BL/6NTac mice over 4 weeks showed no significant immunological reaction and minimal formation of fibrotic capsules.
27109145	8	18	theme	fibroblasts	1391:1401	arg1	proliferation					1369:1381	proliferation	1369:1381	proliferation of L929 fibroblasts	1369:1401	Higher proportions of keratin on coated two-dimensional surfaces and in three-dimensional sponges resulted in more attachment and improved proliferation of L929 fibroblasts, verifying the bioactive role of keratin in the composites.
27109145	11	19	theme	mechanical	1899:1908	arg1	properties					1910:1919	tuneable physical and mechanical properties	1877:1919	tuneable physical and mechanical properties	1877:1919	These findings demonstrated the feasibility of producing crosslinked human hair keratin-alginate sponges, with tuneable physical and mechanical properties, which are cell compliant in vitro and biocompatible in vivo, suggesting their potential for clinically relevant exploitations.
27109145	7	20	theme	water	1096:1100	arg1	capacities					1109:1118	lower water uptake capacities	1090:1118	lower water uptake capacities	1090:1118	Meanwhile, sponges with higher proportions of keratin showed lower water uptake capacities and higher degradation rates by proteinase K, in comparison with sponges with higher proportions of alginate.
27109145	7	21	with	sponges	1040:1046	arg1	proportions					1060:1070	higher proportions	1053:1070	higher proportions of keratin	1053:1081	Meanwhile, sponges with higher proportions of keratin showed lower water uptake capacities and higher degradation rates by proteinase K, in comparison with sponges with higher proportions of alginate.
27109145	3	22	theme	novel	341:345	arg1	sponge					357:362	a novel composite sponge	339:362	a novel composite sponge	339:362	Herein, we described a novel composite sponge, consisting of human hair keratins chemically crosslinked with alginate using 1-ethyl-3-dimethylaminopropyl carbodiimide hydrochloride, with improved mechanical properties.
27109145	6	23	link	crosslinked	917:927	arg1	sponges					929:935	The crosslinked sponges	913:935	The crosslinked sponges	913:935	The crosslinked sponges also demonstrated water vapour transmission rates comparable to commercial wound dressings.
27109145	11	24	theme	physical	1886:1893	arg1	properties					1910:1919	tuneable physical and mechanical properties	1877:1919	tuneable physical and mechanical properties	1877:1919	These findings demonstrated the feasibility of producing crosslinked human hair keratin-alginate sponges, with tuneable physical and mechanical properties, which are cell compliant in vitro and biocompatible in vivo, suggesting their potential for clinically relevant exploitations.
27109145	0	25	theme	keratin-alginate	62:77	arg1	sponge					79:84	a novel crosslinked human keratin-alginate sponge	36:84	a novel crosslinked human keratin-alginate sponge	36:84	Fabrication and characterization of a novel crosslinked human keratin-alginate sponge.
27109145	11	26	dep	compliant	1937:1945	arg1	in					1974:1975	in	1974:1975	in	1974:1975	These findings demonstrated the feasibility of producing crosslinked human hair keratin-alginate sponges, with tuneable physical and mechanical properties, which are cell compliant in vitro and biocompatible in vivo, suggesting their potential for clinically relevant exploitations.
27109145	11	26	dep	compliant	1937:1945	arg1	in					1947:1948	in	1947:1948	in	1947:1948	These findings demonstrated the feasibility of producing crosslinked human hair keratin-alginate sponges, with tuneable physical and mechanical properties, which are cell compliant in vitro and biocompatible in vivo, suggesting their potential for clinically relevant exploitations.
27109145	2	27	theme	mechanical	256:265	arg1	properties					267:276	poor mechanical properties	251:276	poor mechanical properties	251:276	However, pure keratin templates have poor mechanical properties, which limit their practical relevance.
27109145	5	28	theme	tensile	760:766	arg1	strength					768:775	increased tensile strength	750:775	increased tensile strength	750:775	With increasing alginate proportions, the sponges exhibited increased tensile strength, tensile modulus and compression modulus at maximum values of 10.3 ± 1.92 kPa, 219.07 ± 52.39 kPa and 191.48 ± 32.89 kPa, respectively.
27109145	8	29	theme	bioactive	1418:1426	arg1	role					1428:1431	the bioactive role	1414:1431	the bioactive role of keratin in the composites	1414:1460	Higher proportions of keratin on coated two-dimensional surfaces and in three-dimensional sponges resulted in more attachment and improved proliferation of L929 fibroblasts, verifying the bioactive role of keratin in the composites.
27109145	9	30	theme	fibrotic	1639:1646	arg1	capsules					1648:1655	fibrotic capsules	1639:1655	fibrotic capsules	1639:1655	In addition, subcutaneous implantation of the keratin-alginate sponges into C57BL/6NTac mice over 4 weeks showed no significant immunological reaction and minimal formation of fibrotic capsules.
27109145	5	31	theme	compression	798:808	arg1	modulus					810:816	compression modulus	798:816	compression modulus	798:816	With increasing alginate proportions, the sponges exhibited increased tensile strength, tensile modulus and compression modulus at maximum values of 10.3 ± 1.92 kPa, 219.07 ± 52.39 kPa and 191.48 ± 32.89 kPa, respectively.
27109145	0	32	theme	novel	38:42	arg1	sponge					79:84	a novel crosslinked human keratin-alginate sponge	36:84	a novel crosslinked human keratin-alginate sponge	36:84	Fabrication and characterization of a novel crosslinked human keratin-alginate sponge.
27109145	1	33	theme	Human	87:91	arg1	keratins					98:105	Human hair keratins	87:105	Human hair keratins	87:105	Human hair keratins have been explored for biomedical applications because of their abundance, bioactivity and processability.
27109145	5	34	theme	maximum	821:827	arg1	values					829:834	maximum values	821:834	maximum values	821:834	With increasing alginate proportions, the sponges exhibited increased tensile strength, tensile modulus and compression modulus at maximum values of 10.3 ± 1.92 kPa, 219.07 ± 52.39 kPa and 191.48 ± 32.89 kPa, respectively.
27109145	3	35	theme	1-ethyl-3-dimethylaminopropyl	442:470	arg1	hydrochloride					485:497	1-ethyl-3-dimethylaminopropyl carbodiimide hydrochloride	442:497	1-ethyl-3-dimethylaminopropyl carbodiimide hydrochloride	442:497	Herein, we described a novel composite sponge, consisting of human hair keratins chemically crosslinked with alginate using 1-ethyl-3-dimethylaminopropyl carbodiimide hydrochloride, with improved mechanical properties.
27109145	5	36	theme	±	863:863	arg1	kPa					871:873	219.07 ± 52.39 kPa	856:873	219.07 ± 52.39 kPa	856:873	With increasing alginate proportions, the sponges exhibited increased tensile strength, tensile modulus and compression modulus at maximum values of 10.3 ± 1.92 kPa, 219.07 ± 52.39 kPa and 191.48 ± 32.89 kPa, respectively.
27109145	3	37	theme	human	379:383	arg1	keratins					390:397	human hair keratins	379:397	human hair keratins chemically crosslinked with alginate using 1-ethyl-3-dimethylaminopropyl carbodiimide hydrochloride	379:497	Herein, we described a novel composite sponge, consisting of human hair keratins chemically crosslinked with alginate using 1-ethyl-3-dimethylaminopropyl carbodiimide hydrochloride, with improved mechanical properties.
27109145	3	38	theme	mechanical	514:523	arg1	properties					525:534	improved mechanical properties	505:534	improved mechanical properties	505:534	Herein, we described a novel composite sponge, consisting of human hair keratins chemically crosslinked with alginate using 1-ethyl-3-dimethylaminopropyl carbodiimide hydrochloride, with improved mechanical properties.
27109145	5	39	theme	kPa	871:873	arg1	values					829:834	maximum values	821:834	maximum values	821:834	With increasing alginate proportions, the sponges exhibited increased tensile strength, tensile modulus and compression modulus at maximum values of 10.3 ± 1.92 kPa, 219.07 ± 52.39 kPa and 191.48 ± 32.89 kPa, respectively.
27109145	4	40	dep	Fourier	537:543	arg1	transform					545:553	transform	545:553	transform infrared spectroscopy (FTIR) and free amine group quantification using ninhydrin	545:634	Fourier transform infrared spectroscopy (FTIR) and free amine group quantification using ninhydrin revealed a maximum crosslinking index of 82.1 ± 1.3%.
27109145	11	41	theme	keratin-alginate	1846:1861	arg1	sponges					1863:1869	crosslinked human hair keratin-alginate sponges	1823:1869	crosslinked human hair keratin-alginate sponges	1823:1869	These findings demonstrated the feasibility of producing crosslinked human hair keratin-alginate sponges, with tuneable physical and mechanical properties, which are cell compliant in vitro and biocompatible in vivo, suggesting their potential for clinically relevant exploitations.
27109145	11	42	theme	human	1835:1839	arg1	sponges					1863:1869	crosslinked human hair keratin-alginate sponges	1823:1869	crosslinked human hair keratin-alginate sponges	1823:1869	These findings demonstrated the feasibility of producing crosslinked human hair keratin-alginate sponges, with tuneable physical and mechanical properties, which are cell compliant in vitro and biocompatible in vivo, suggesting their potential for clinically relevant exploitations.
27109145	7	43	theme	higher	1198:1203	arg1	proportions					1205:1215	higher proportions	1198:1215	higher proportions of alginate	1198:1227	Meanwhile, sponges with higher proportions of keratin showed lower water uptake capacities and higher degradation rates by proteinase K, in comparison with sponges with higher proportions of alginate.
27109145	8	44	from	proportions	1237:1247	arg1	surfaces					1286:1293	coated two-dimensional surfaces	1263:1293	coated two-dimensional surfaces	1263:1293	Higher proportions of keratin on coated two-dimensional surfaces and in three-dimensional sponges resulted in more attachment and improved proliferation of L929 fibroblasts, verifying the bioactive role of keratin in the composites.
27109145	8	44	from	proportions	1237:1247	arg1	sponges					1320:1326	three-dimensional sponges	1302:1326	three-dimensional sponges	1302:1326	Higher proportions of keratin on coated two-dimensional surfaces and in three-dimensional sponges resulted in more attachment and improved proliferation of L929 fibroblasts, verifying the bioactive role of keratin in the composites.
27109145	4	45	theme	amine	593:597	arg1	quantification					605:618	free amine group quantification	588:618	free amine group quantification	588:618	Fourier transform infrared spectroscopy (FTIR) and free amine group quantification using ninhydrin revealed a maximum crosslinking index of 82.1 ± 1.3%.
27109145	11	46	theme	tuneable	1877:1884	arg1	properties					1910:1919	tuneable physical and mechanical properties	1877:1919	tuneable physical and mechanical properties	1877:1919	These findings demonstrated the feasibility of producing crosslinked human hair keratin-alginate sponges, with tuneable physical and mechanical properties, which are cell compliant in vitro and biocompatible in vivo, suggesting their potential for clinically relevant exploitations.
27109145	8	47	from	role	1428:1431	arg1	composites					1451:1460	the composites	1447:1460	the composites	1447:1460	Higher proportions of keratin on coated two-dimensional surfaces and in three-dimensional sponges resulted in more attachment and improved proliferation of L929 fibroblasts, verifying the bioactive role of keratin in the composites.
27109145	9	48	theme	subcutaneous	1476:1487	arg1	implantation					1489:1500	subcutaneous implantation	1476:1500	subcutaneous implantation of the keratin-alginate sponges into C57BL/6NTac mice over 4 weeks	1476:1567	In addition, subcutaneous implantation of the keratin-alginate sponges into C57BL/6NTac mice over 4 weeks showed no significant immunological reaction and minimal formation of fibrotic capsules.
27109145	0	49	theme	human	56:60	arg1	sponge					79:84	a novel crosslinked human keratin-alginate sponge	36:84	a novel crosslinked human keratin-alginate sponge	36:84	Fabrication and characterization of a novel crosslinked human keratin-alginate sponge.
27109145	6	50	theme	transmission	968:979	arg1	rates					981:985	water vapour transmission rates	955:985	water vapour transmission rates comparable to commercial wound dressings	955:1026	The crosslinked sponges also demonstrated water vapour transmission rates comparable to commercial wound dressings.
27109145	7	51	with	comparison	1169:1178	arg1	sponges					1185:1191	sponges	1185:1191	sponges with higher proportions of alginate	1185:1227	Meanwhile, sponges with higher proportions of keratin showed lower water uptake capacities and higher degradation rates by proteinase K, in comparison with sponges with higher proportions of alginate.
27109145	11	52	link	crosslinked	1823:1833	arg1	sponges					1863:1869	crosslinked human hair keratin-alginate sponges	1823:1869	crosslinked human hair keratin-alginate sponges	1823:1869	These findings demonstrated the feasibility of producing crosslinked human hair keratin-alginate sponges, with tuneable physical and mechanical properties, which are cell compliant in vitro and biocompatible in vivo, suggesting their potential for clinically relevant exploitations.
27109145	4	53	theme	±	682:682	arg1	%					687:687	82.1 ± 1.3%	677:687	82.1 ± 1.3%	677:687	Fourier transform infrared spectroscopy (FTIR) and free amine group quantification using ninhydrin revealed a maximum crosslinking index of 82.1 ± 1.3%.
27109145	5	54	theme	alginate	706:713	arg1	proportions					715:725	alginate proportions	706:725	alginate proportions	706:725	With increasing alginate proportions, the sponges exhibited increased tensile strength, tensile modulus and compression modulus at maximum values of 10.3 ± 1.92 kPa, 219.07 ± 52.39 kPa and 191.48 ± 32.89 kPa, respectively.
27109145	6	55	theme	water	955:959	arg1	rates					981:985	water vapour transmission rates	955:985	water vapour transmission rates comparable to commercial wound dressings	955:1026	The crosslinked sponges also demonstrated water vapour transmission rates comparable to commercial wound dressings.
27109145	7	56	with	sponges	1185:1191	arg1	proportions					1205:1215	higher proportions	1198:1215	higher proportions of alginate	1198:1227	Meanwhile, sponges with higher proportions of keratin showed lower water uptake capacities and higher degradation rates by proteinase K, in comparison with sponges with higher proportions of alginate.
27109145	10	57	theme	cellular	1693:1700	arg1	infiltration					1702:1713	cellular infiltration	1693:1713	cellular infiltration	1693:1713	Furthermore, the sponges supported cellular infiltration, neo-tissue formation and vascularization in vivo.
27109145	9	58	theme	keratin-alginate	1509:1524	arg1	sponges					1526:1532	the keratin-alginate sponges	1505:1532	the keratin-alginate sponges	1505:1532	In addition, subcutaneous implantation of the keratin-alginate sponges into C57BL/6NTac mice over 4 weeks showed no significant immunological reaction and minimal formation of fibrotic capsules.
27109145	4	59	theme	crosslinking	655:666	arg1	index					668:672	a maximum crosslinking index	645:672	a maximum crosslinking index of 82.1 ± 1.3%	645:687	Fourier transform infrared spectroscopy (FTIR) and free amine group quantification using ninhydrin revealed a maximum crosslinking index of 82.1 ± 1.3%.
27109145	5	60	theme	kPa	894:896	arg1	values					829:834	maximum values	821:834	maximum values	821:834	With increasing alginate proportions, the sponges exhibited increased tensile strength, tensile modulus and compression modulus at maximum values of 10.3 ± 1.92 kPa, 219.07 ± 52.39 kPa and 191.48 ± 32.89 kPa, respectively.
27109145	8	61	theme	Higher	1230:1235	arg1	proportions					1237:1247	Higher proportions	1230:1247	Higher proportions of keratin on coated two-dimensional surfaces and in three-dimensional sponges	1230:1326	Higher proportions of keratin on coated two-dimensional surfaces and in three-dimensional sponges resulted in more attachment and improved proliferation of L929 fibroblasts, verifying the bioactive role of keratin in the composites.
27109145	6	62	theme	crosslinked	917:927	arg1	sponges					929:935	The crosslinked sponges	913:935	The crosslinked sponges	913:935	The crosslinked sponges also demonstrated water vapour transmission rates comparable to commercial wound dressings.
27109145	8	63	theme	three-dimensional	1302:1318	arg1	sponges					1320:1326	three-dimensional sponges	1302:1326	three-dimensional sponges	1302:1326	Higher proportions of keratin on coated two-dimensional surfaces and in three-dimensional sponges resulted in more attachment and improved proliferation of L929 fibroblasts, verifying the bioactive role of keratin in the composites.
27109145	0	64	theme	crosslinked	44:54	arg1	sponge					79:84	a novel crosslinked human keratin-alginate sponge	36:84	a novel crosslinked human keratin-alginate sponge	36:84	Fabrication and characterization of a novel crosslinked human keratin-alginate sponge.
27109145	7	65	theme	degradation	1131:1141	arg1	rates					1143:1147	higher degradation rates	1124:1147	higher degradation rates	1124:1147	Meanwhile, sponges with higher proportions of keratin showed lower water uptake capacities and higher degradation rates by proteinase K, in comparison with sponges with higher proportions of alginate.
27109145	8	66	theme	more	1340:1343	arg1	attachment					1345:1354	more attachment	1340:1354	more attachment	1340:1354	Higher proportions of keratin on coated two-dimensional surfaces and in three-dimensional sponges resulted in more attachment and improved proliferation of L929 fibroblasts, verifying the bioactive role of keratin in the composites.
27109145	5	67	theme	tensile	778:784	arg1	modulus					786:792	tensile modulus	778:792	tensile modulus	778:792	With increasing alginate proportions, the sponges exhibited increased tensile strength, tensile modulus and compression modulus at maximum values of 10.3 ± 1.92 kPa, 219.07 ± 52.39 kPa and 191.48 ± 32.89 kPa, respectively.
27109145	2	68	theme	poor	251:254	arg1	properties					267:276	poor mechanical properties	251:276	poor mechanical properties	251:276	However, pure keratin templates have poor mechanical properties, which limit their practical relevance.
27109145	5	69	theme	±	886:886	arg1	kPa					894:896	191.48 ± 32.89 kPa	879:896	191.48 ± 32.89 kPa	879:896	With increasing alginate proportions, the sponges exhibited increased tensile strength, tensile modulus and compression modulus at maximum values of 10.3 ± 1.92 kPa, 219.07 ± 52.39 kPa and 191.48 ± 32.89 kPa, respectively.
27109145	8	70	theme	two-dimensional	1270:1284	arg1	surfaces					1286:1293	coated two-dimensional surfaces	1263:1293	coated two-dimensional surfaces	1263:1293	Higher proportions of keratin on coated two-dimensional surfaces and in three-dimensional sponges resulted in more attachment and improved proliferation of L929 fibroblasts, verifying the bioactive role of keratin in the composites.
27109145	6	71	theme	commercial	1001:1010	arg1	dressings					1018:1026	commercial wound dressings	1001:1026	commercial wound dressings	1001:1026	The crosslinked sponges also demonstrated water vapour transmission rates comparable to commercial wound dressings.
27109145	12	72	dep	&	2077:2077	arg1	Sons					2079:2082	Sons	2079:2082	Sons	2079:2082	Copyright © 2016 John Wiley & Sons, Ltd.
27109145	7	73	theme	uptake	1102:1107	arg1	capacities					1109:1118	lower water uptake capacities	1090:1118	lower water uptake capacities	1090:1118	Meanwhile, sponges with higher proportions of keratin showed lower water uptake capacities and higher degradation rates by proteinase K, in comparison with sponges with higher proportions of alginate.
27109145	9	74	theme	significant	1579:1589	arg1	reaction					1605:1612	no significant immunological reaction	1576:1612	no significant immunological reaction	1576:1612	In addition, subcutaneous implantation of the keratin-alginate sponges into C57BL/6NTac mice over 4 weeks showed no significant immunological reaction and minimal formation of fibrotic capsules.
27109145	6	75	theme	comparable	987:996	arg1	rates					981:985	water vapour transmission rates	955:985	water vapour transmission rates comparable to commercial wound dressings	955:1026	The crosslinked sponges also demonstrated water vapour transmission rates comparable to commercial wound dressings.
27109145	7	76	theme	lower	1090:1094	arg1	capacities					1109:1118	lower water uptake capacities	1090:1118	lower water uptake capacities	1090:1118	Meanwhile, sponges with higher proportions of keratin showed lower water uptake capacities and higher degradation rates by proteinase K, in comparison with sponges with higher proportions of alginate.
27109145	1	77	theme	biomedical	130:139	arg1	applications					141:152	biomedical applications	130:152	biomedical applications	130:152	Human hair keratins have been explored for biomedical applications because of their abundance, bioactivity and processability.
27109145	8	78	theme	L929	1386:1389	arg1	fibroblasts					1391:1401	L929 fibroblasts	1386:1401	L929 fibroblasts	1386:1401	Higher proportions of keratin on coated two-dimensional surfaces and in three-dimensional sponges resulted in more attachment and improved proliferation of L929 fibroblasts, verifying the bioactive role of keratin in the composites.
27109145	0	79	theme	sponge	79:84	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Fabrication and characterization of a novel crosslinked human keratin-alginate sponge.
27109145	0	79	theme	sponge	79:84	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication	0:10	Fabrication and characterization of a novel crosslinked human keratin-alginate sponge.
27109145	7	80	theme	higher	1053:1058	arg1	proportions					1060:1070	higher proportions	1053:1070	higher proportions of keratin	1053:1081	Meanwhile, sponges with higher proportions of keratin showed lower water uptake capacities and higher degradation rates by proteinase K, in comparison with sponges with higher proportions of alginate.
27109145	9	81	theme	minimal	1618:1624	arg1	formation					1626:1634	minimal formation	1618:1634	minimal formation of fibrotic capsules	1618:1655	In addition, subcutaneous implantation of the keratin-alginate sponges into C57BL/6NTac mice over 4 weeks showed no significant immunological reaction and minimal formation of fibrotic capsules.
27109145	3	82	theme	composite	347:355	arg1	sponge					357:362	a novel composite sponge	339:362	a novel composite sponge	339:362	Herein, we described a novel composite sponge, consisting of human hair keratins chemically crosslinked with alginate using 1-ethyl-3-dimethylaminopropyl carbodiimide hydrochloride, with improved mechanical properties.
27109145	11	83	dep	in	1947:1948	arg1	vitro					1950:1954	vitro	1950:1954	vitro	1950:1954	These findings demonstrated the feasibility of producing crosslinked human hair keratin-alginate sponges, with tuneable physical and mechanical properties, which are cell compliant in vitro and biocompatible in vivo, suggesting their potential for clinically relevant exploitations.
27109145	9	84	theme	capsules	1648:1655	arg1	reaction					1605:1612	no significant immunological reaction	1576:1612	no significant immunological reaction	1576:1612	In addition, subcutaneous implantation of the keratin-alginate sponges into C57BL/6NTac mice over 4 weeks showed no significant immunological reaction and minimal formation of fibrotic capsules.
27109145	9	84	theme	capsules	1648:1655	arg1	formation					1626:1634	minimal formation	1618:1634	minimal formation of fibrotic capsules	1618:1655	In addition, subcutaneous implantation of the keratin-alginate sponges into C57BL/6NTac mice over 4 weeks showed no significant immunological reaction and minimal formation of fibrotic capsules.
27109145	11	85	dep	in	1974:1975	arg1	vivo					1977:1980	vivo	1977:1980	vivo	1977:1980	These findings demonstrated the feasibility of producing crosslinked human hair keratin-alginate sponges, with tuneable physical and mechanical properties, which are cell compliant in vitro and biocompatible in vivo, suggesting their potential for clinically relevant exploitations.
27109145	4	86	theme	%	687:687	arg1	index					668:672	a maximum crosslinking index	645:672	a maximum crosslinking index of 82.1 ± 1.3%	645:687	Fourier transform infrared spectroscopy (FTIR) and free amine group quantification using ninhydrin revealed a maximum crosslinking index of 82.1 ± 1.3%.
27109145	8	87	theme	keratin	1436:1442	arg1	role					1428:1431	the bioactive role	1414:1431	the bioactive role of keratin in the composites	1414:1460	Higher proportions of keratin on coated two-dimensional surfaces and in three-dimensional sponges resulted in more attachment and improved proliferation of L929 fibroblasts, verifying the bioactive role of keratin in the composites.
27109145	1	88	theme	hair	93:96	arg1	keratins					98:105	Human hair keratins	87:105	Human hair keratins	87:105	Human hair keratins have been explored for biomedical applications because of their abundance, bioactivity and processability.
27109145	3	89	theme	carbodiimide	472:483	arg1	hydrochloride					485:497	1-ethyl-3-dimethylaminopropyl carbodiimide hydrochloride	442:497	1-ethyl-3-dimethylaminopropyl carbodiimide hydrochloride	442:497	Herein, we described a novel composite sponge, consisting of human hair keratins chemically crosslinked with alginate using 1-ethyl-3-dimethylaminopropyl carbodiimide hydrochloride, with improved mechanical properties.
27109145	7	90	theme	alginate	1220:1227	arg1	proportions					1205:1215	higher proportions	1198:1215	higher proportions of alginate	1198:1227	Meanwhile, sponges with higher proportions of keratin showed lower water uptake capacities and higher degradation rates by proteinase K, in comparison with sponges with higher proportions of alginate.
27109145	4	91	dep	transform	545:553	arg1	infrared					555:562	infrared	555:562	transform infrared spectroscopy (FTIR) and free amine group quantification using ninhydrin	545:634	Fourier transform infrared spectroscopy (FTIR) and free amine group quantification using ninhydrin revealed a maximum crosslinking index of 82.1 ± 1.3%.
27109145	3	92	theme	hair	385:388	arg1	keratins					390:397	human hair keratins	379:397	human hair keratins chemically crosslinked with alginate using 1-ethyl-3-dimethylaminopropyl carbodiimide hydrochloride	379:497	Herein, we described a novel composite sponge, consisting of human hair keratins chemically crosslinked with alginate using 1-ethyl-3-dimethylaminopropyl carbodiimide hydrochloride, with improved mechanical properties.
27109145	11	93	theme	hair	1841:1844	arg1	sponges					1863:1869	crosslinked human hair keratin-alginate sponges	1823:1869	crosslinked human hair keratin-alginate sponges	1823:1869	These findings demonstrated the feasibility of producing crosslinked human hair keratin-alginate sponges, with tuneable physical and mechanical properties, which are cell compliant in vitro and biocompatible in vivo, suggesting their potential for clinically relevant exploitations.
27109145	5	94	theme	±	844:844	arg1	kPa					851:853	10.3 ± 1.92 kPa	839:853	10.3 ± 1.92 kPa	839:853	With increasing alginate proportions, the sponges exhibited increased tensile strength, tensile modulus and compression modulus at maximum values of 10.3 ± 1.92 kPa, 219.07 ± 52.39 kPa and 191.48 ± 32.89 kPa, respectively.
27109145	4	95	theme	free	588:591	arg1	quantification					605:618	free amine group quantification	588:618	free amine group quantification	588:618	Fourier transform infrared spectroscopy (FTIR) and free amine group quantification using ninhydrin revealed a maximum crosslinking index of 82.1 ± 1.3%.
27109145	11	96	theme	crosslinked	1823:1833	arg1	sponges					1863:1869	crosslinked human hair keratin-alginate sponges	1823:1869	crosslinked human hair keratin-alginate sponges	1823:1869	These findings demonstrated the feasibility of producing crosslinked human hair keratin-alginate sponges, with tuneable physical and mechanical properties, which are cell compliant in vitro and biocompatible in vivo, suggesting their potential for clinically relevant exploitations.
27109145	2	97	contain	have	246:249	arg2	properties					267:276	poor mechanical properties	251:276	poor mechanical properties	251:276	However, pure keratin templates have poor mechanical properties, which limit their practical relevance.
27109145	2	97	contain	have	246:249	arg1	templates					236:244	pure keratin templates	223:244	pure keratin templates	223:244	However, pure keratin templates have poor mechanical properties, which limit their practical relevance.
27109145	3	98	theme	improved	505:512	arg1	properties					525:534	improved mechanical properties	505:534	improved mechanical properties	505:534	Herein, we described a novel composite sponge, consisting of human hair keratins chemically crosslinked with alginate using 1-ethyl-3-dimethylaminopropyl carbodiimide hydrochloride, with improved mechanical properties.
27109145	5	99	theme	kPa	851:853	arg1	values					829:834	maximum values	821:834	maximum values	821:834	With increasing alginate proportions, the sponges exhibited increased tensile strength, tensile modulus and compression modulus at maximum values of 10.3 ± 1.92 kPa, 219.07 ± 52.39 kPa and 191.48 ± 32.89 kPa, respectively.
27109145	4	100	theme	group	599:603	arg1	quantification					605:618	free amine group quantification	588:618	free amine group quantification	588:618	Fourier transform infrared spectroscopy (FTIR) and free amine group quantification using ninhydrin revealed a maximum crosslinking index of 82.1 ± 1.3%.
27109145	2	101	theme	pure	223:226	arg1	templates					236:244	pure keratin templates	223:244	pure keratin templates	223:244	However, pure keratin templates have poor mechanical properties, which limit their practical relevance.
24310794	10	0	theme	due	1709:1711	arg1	differences					1629:1639	slight differences	1622:1639	slight differences	1622:1639	Our results indicate that slight differences in the composition of heparins' non-anticoagulant oligosaccharides, due to different origins of material and preparation methods, have the potential to cause diverse effects and highlight the need for additional characterisation of non-anticoagulant activities.
24310794	10	1	theme	slight	1622:1627	arg1	differences					1629:1639	slight differences	1622:1639	slight differences	1622:1639	Our results indicate that slight differences in the composition of heparins' non-anticoagulant oligosaccharides, due to different origins of material and preparation methods, have the potential to cause diverse effects and highlight the need for additional characterisation of non-anticoagulant activities.
24310794	2	2	theme	effects	361:367	arg1	modulation					384:393	the modulation	380:393	the modulation of cellular growth	380:412	In addition to their well-known anticoagulant effect, heparins have shown to mediate a wide range of non-anticoagulant effects, including the modulation of cellular growth.
24310794	2	2	theme	effects	361:367	arg1	range					334:338	a wide range	327:338	a wide range of non-anticoagulant effects, including the modulation of cellular growth	327:412	In addition to their well-known anticoagulant effect, heparins have shown to mediate a wide range of non-anticoagulant effects, including the modulation of cellular growth.
24310794	5	3	from	changes	985:991	arg1	viability					1001:1009	cell viability	996:1009	cell viability	996:1009	In our experimental setting, all heparins caused a dose-dependent reduction in cellular growth, which correlated well with the induction of cell cycle arrest in the G₁ phase and which was not associated with significant changes in cell viability.
24310794	5	4	theme	significant	973:983	arg1	changes					985:991	significant changes	973:991	significant changes in cell viability	973:1009	In our experimental setting, all heparins caused a dose-dependent reduction in cellular growth, which correlated well with the induction of cell cycle arrest in the G₁ phase and which was not associated with significant changes in cell viability.
24310794	9	5	theme	other	1560:1564	arg1	properties					1584:1593	other non-anticoagulant properties	1560:1593	other non-anticoagulant properties	1560:1593	Notably, heparins are currently standardised only according to their anticoagulant activity but not based on other non-anticoagulant properties.
24310794	10	6	theme	material	1737:1744	arg1	origins					1726:1732	different origins	1716:1732	different origins of material and preparation methods	1716:1768	Our results indicate that slight differences in the composition of heparins' non-anticoagulant oligosaccharides, due to different origins of material and preparation methods, have the potential to cause diverse effects and highlight the need for additional characterisation of non-anticoagulant activities.
24310794	2	7	theme	wide	329:332	arg1	modulation					384:393	the modulation	380:393	the modulation of cellular growth	380:412	In addition to their well-known anticoagulant effect, heparins have shown to mediate a wide range of non-anticoagulant effects, including the modulation of cellular growth.
24310794	2	7	theme	wide	329:332	arg1	range					334:338	a wide range	327:338	a wide range of non-anticoagulant effects, including the modulation of cellular growth	327:412	In addition to their well-known anticoagulant effect, heparins have shown to mediate a wide range of non-anticoagulant effects, including the modulation of cellular growth.
24310794	5	8	theme	experimental	772:783	arg1	setting					785:791	our experimental setting	768:791	our experimental setting	768:791	In our experimental setting, all heparins caused a dose-dependent reduction in cellular growth, which correlated well with the induction of cell cycle arrest in the G₁ phase and which was not associated with significant changes in cell viability.
24310794	3	9	theme	cellular	497:504	arg1	proliferation					506:518	cellular proliferation	497:518	cellular proliferation	497:518	However, contradictory results have been reported with regard to their effects on cellular proliferation, with some studies suggesting anti-proliferative while others indicating pro-proliferative effects.
24310794	4	10	theme	epithelial	679:688	arg1	cells					697:701	human colonic epithelial cancer cells	665:701	human colonic epithelial cancer cells	665:701	This study investigated the proliferation of human colonic epithelial cancer cells in the presence of UFH and LMWHs (enoxaparin and dalteparin).
24310794	4	11	dep	LMWHs	730:734	arg1	dalteparin					752:761	dalteparin	752:761	dalteparin	752:761	This study investigated the proliferation of human colonic epithelial cancer cells in the presence of UFH and LMWHs (enoxaparin and dalteparin).
24310794	4	11	dep	LMWHs	730:734	arg1	enoxaparin					737:746	enoxaparin	737:746	enoxaparin	737:746	This study investigated the proliferation of human colonic epithelial cancer cells in the presence of UFH and LMWHs (enoxaparin and dalteparin).
24310794	4	11	dep	LMWHs	730:734	arg1	LMWHs					730:734	LMWHs	730:734	LMWHs (enoxaparin and dalteparin)	730:762	This study investigated the proliferation of human colonic epithelial cancer cells in the presence of UFH and LMWHs (enoxaparin and dalteparin).
24310794	5	12	theme	cell	905:908	arg1	arrest					916:921	cell cycle arrest	905:921	cell cycle arrest	905:921	In our experimental setting, all heparins caused a dose-dependent reduction in cellular growth, which correlated well with the induction of cell cycle arrest in the G₁ phase and which was not associated with significant changes in cell viability.
24310794	2	13	theme	growth	407:412	arg1	modulation					384:393	the modulation	380:393	the modulation of cellular growth	380:412	In addition to their well-known anticoagulant effect, heparins have shown to mediate a wide range of non-anticoagulant effects, including the modulation of cellular growth.
24310794	7	14	theme	oligosaccharides	1270:1285	arg1	majority					1258:1265	the majority	1254:1265	the majority of oligosaccharides	1254:1285	Surprisingly, only two oligosaccharides showed distinctive anti-proliferative effects while the majority of oligosaccharides actually stimulated proliferation.
24310794	5	15	theme	G₁	930:931	arg1	phase					933:937	the G₁ phase	926:937	the G₁ phase	926:937	In our experimental setting, all heparins caused a dose-dependent reduction in cellular growth, which correlated well with the induction of cell cycle arrest in the G₁ phase and which was not associated with significant changes in cell viability.
24310794	7	16	theme	distinctive	1209:1219	arg1	effects					1240:1246	distinctive anti-proliferative effects	1209:1246	distinctive anti-proliferative effects	1209:1246	Surprisingly, only two oligosaccharides showed distinctive anti-proliferative effects while the majority of oligosaccharides actually stimulated proliferation.
24310794	3	17	from	effects	486:492	arg1	proliferation					506:518	cellular proliferation	497:518	cellular proliferation	497:518	However, contradictory results have been reported with regard to their effects on cellular proliferation, with some studies suggesting anti-proliferative while others indicating pro-proliferative effects.
24310794	1	18	theme	unfractionated	86:99	arg1	UFH					110:112	UFH	110:112	UFH	110:112	Heparins, unfractionated heparin (UFH) and low molecular weight heparins (LMWHs), are heterogeneous mixtures of anticoagulant and non-anticoagulant oligosaccharides.
24310794	1	18	theme	unfractionated	86:99	arg1	Heparins					76:83	Heparins	76:83	Heparins	76:83	Heparins, unfractionated heparin (UFH) and low molecular weight heparins (LMWHs), are heterogeneous mixtures of anticoagulant and non-anticoagulant oligosaccharides.
24310794	1	18	theme	unfractionated	86:99	arg1	heparin					101:107	unfractionated heparin	86:107	unfractionated heparin (UFH)	86:113	Heparins, unfractionated heparin (UFH) and low molecular weight heparins (LMWHs), are heterogeneous mixtures of anticoagulant and non-anticoagulant oligosaccharides.
24310794	2	19	theme	cellular	398:405	arg1	growth					407:412	cellular growth	398:412	cellular growth	398:412	In addition to their well-known anticoagulant effect, heparins have shown to mediate a wide range of non-anticoagulant effects, including the modulation of cellular growth.
24310794	5	20	theme	cellular	844:851	arg1	growth					853:858	cellular growth	844:858	cellular growth	844:858	In our experimental setting, all heparins caused a dose-dependent reduction in cellular growth, which correlated well with the induction of cell cycle arrest in the G₁ phase and which was not associated with significant changes in cell viability.
24310794	1	21	theme	heterogeneous	162:174	arg1	mixtures					176:183	heterogeneous mixtures	162:183	heterogeneous mixtures of anticoagulant and non-anticoagulant oligosaccharides	162:239	Heparins, unfractionated heparin (UFH) and low molecular weight heparins (LMWHs), are heterogeneous mixtures of anticoagulant and non-anticoagulant oligosaccharides.
24310794	1	21	theme	heterogeneous	162:174	arg1	Heparins					76:83	Heparins	76:83	Heparins	76:83	Heparins, unfractionated heparin (UFH) and low molecular weight heparins (LMWHs), are heterogeneous mixtures of anticoagulant and non-anticoagulant oligosaccharides.
24310794	7	22	theme	anti-proliferative	1221:1238	arg1	effects					1240:1246	distinctive anti-proliferative effects	1209:1246	distinctive anti-proliferative effects	1209:1246	Surprisingly, only two oligosaccharides showed distinctive anti-proliferative effects while the majority of oligosaccharides actually stimulated proliferation.
24310794	6	23	from	effects	1016:1022	arg1	proliferation					1036:1048	cellular proliferation	1027:1048	cellular proliferation of 14 different oligosaccharides of enoxaparin obtained through ion-exchange chromatography	1027:1140	The effects on cellular proliferation of 14 different oligosaccharides of enoxaparin obtained through ion-exchange chromatography were also assessed.
24310794	5	24	theme	cycle	910:914	arg1	arrest					916:921	cell cycle arrest	905:921	cell cycle arrest	905:921	In our experimental setting, all heparins caused a dose-dependent reduction in cellular growth, which correlated well with the induction of cell cycle arrest in the G₁ phase and which was not associated with significant changes in cell viability.
24310794	10	25	theme	methods	1762:1768	arg1	origins					1726:1732	different origins	1716:1732	different origins of material and preparation methods	1716:1768	Our results indicate that slight differences in the composition of heparins' non-anticoagulant oligosaccharides, due to different origins of material and preparation methods, have the potential to cause diverse effects and highlight the need for additional characterisation of non-anticoagulant activities.
24310794	10	26	theme	additional	1842:1851	arg1	characterisation					1853:1868	additional characterisation	1842:1868	additional characterisation of non-anticoagulant activities	1842:1900	Our results indicate that slight differences in the composition of heparins' non-anticoagulant oligosaccharides, due to different origins of material and preparation methods, have the potential to cause diverse effects and highlight the need for additional characterisation of non-anticoagulant activities.
24310794	8	27	theme	strongest	1414:1422	arg1	effect					1443:1448	the strongest anti-proliferative effect	1410:1448	the strongest anti-proliferative effect	1410:1448	Interestingly, the smallest oligosaccharide devoid of any anticoagulant activity showed the strongest anti-proliferative effect.
24310794	4	28	theme	cancer	690:695	arg1	cells					697:701	human colonic epithelial cancer cells	665:701	human colonic epithelial cancer cells	665:701	This study investigated the proliferation of human colonic epithelial cancer cells in the presence of UFH and LMWHs (enoxaparin and dalteparin).
24310794	0	29	theme	pro-	18:21	arg1	oligosaccharides					46:61	pro- and anti-proliferative oligosaccharides	18:61	pro- and anti-proliferative oligosaccharides of heparins	18:73	Identification of pro- and anti-proliferative oligosaccharides of heparins.
24310794	5	30	theme	cell	996:999	arg1	viability					1001:1009	cell viability	996:1009	cell viability	996:1009	In our experimental setting, all heparins caused a dose-dependent reduction in cellular growth, which correlated well with the induction of cell cycle arrest in the G₁ phase and which was not associated with significant changes in cell viability.
24310794	3	31	theme	pro-proliferative	593:609	arg1	effects					611:617	pro-proliferative effects	593:617	pro-proliferative effects	593:617	However, contradictory results have been reported with regard to their effects on cellular proliferation, with some studies suggesting anti-proliferative while others indicating pro-proliferative effects.
24310794	9	32	theme	non-anticoagulant	1566:1582	arg1	properties					1584:1593	other non-anticoagulant properties	1560:1593	other non-anticoagulant properties	1560:1593	Notably, heparins are currently standardised only according to their anticoagulant activity but not based on other non-anticoagulant properties.
24310794	1	33	theme	anticoagulant	188:200	arg1	oligosaccharides					224:239	anticoagulant and non-anticoagulant oligosaccharides	188:239	anticoagulant and non-anticoagulant oligosaccharides	188:239	Heparins, unfractionated heparin (UFH) and low molecular weight heparins (LMWHs), are heterogeneous mixtures of anticoagulant and non-anticoagulant oligosaccharides.
24310794	0	34	theme	oligosaccharides	46:61	arg1	Identification					0:13	Identification	0:13	Identification of pro- and anti-proliferative oligosaccharides of heparins.	0:74	Identification of pro- and anti-proliferative oligosaccharides of heparins.
24310794	10	35	theme	non-anticoagulant	1873:1889	arg1	activities					1891:1900	non-anticoagulant activities	1873:1900	non-anticoagulant activities	1873:1900	Our results indicate that slight differences in the composition of heparins' non-anticoagulant oligosaccharides, due to different origins of material and preparation methods, have the potential to cause diverse effects and highlight the need for additional characterisation of non-anticoagulant activities.
24310794	8	36	theme	activity	1394:1401	arg1	devoid					1366:1371	devoid	1366:1371	devoid	1366:1371	Interestingly, the smallest oligosaccharide devoid of any anticoagulant activity showed the strongest anti-proliferative effect.
24310794	10	37	theme	diverse	1799:1805	arg1	effects					1807:1813	diverse effects	1799:1813	diverse effects	1799:1813	Our results indicate that slight differences in the composition of heparins' non-anticoagulant oligosaccharides, due to different origins of material and preparation methods, have the potential to cause diverse effects and highlight the need for additional characterisation of non-anticoagulant activities.
24310794	6	38	theme	oligosaccharides	1066:1081	arg1	proliferation					1036:1048	cellular proliferation	1027:1048	cellular proliferation of 14 different oligosaccharides of enoxaparin obtained through ion-exchange chromatography	1027:1140	The effects on cellular proliferation of 14 different oligosaccharides of enoxaparin obtained through ion-exchange chromatography were also assessed.
24310794	0	39	theme	anti-proliferative	27:44	arg1	oligosaccharides					46:61	pro- and anti-proliferative oligosaccharides	18:61	pro- and anti-proliferative oligosaccharides of heparins	18:73	Identification of pro- and anti-proliferative oligosaccharides of heparins.
24310794	3	40	theme	contradictory	424:436	arg1	results					438:444	contradictory results	424:444	contradictory results	424:444	However, contradictory results have been reported with regard to their effects on cellular proliferation, with some studies suggesting anti-proliferative while others indicating pro-proliferative effects.
24310794	6	41	theme	cellular	1027:1034	arg1	proliferation					1036:1048	cellular proliferation	1027:1048	cellular proliferation of 14 different oligosaccharides of enoxaparin obtained through ion-exchange chromatography	1027:1140	The effects on cellular proliferation of 14 different oligosaccharides of enoxaparin obtained through ion-exchange chromatography were also assessed.
24310794	1	42	theme	non-anticoagulant	206:222	arg1	oligosaccharides					224:239	anticoagulant and non-anticoagulant oligosaccharides	188:239	anticoagulant and non-anticoagulant oligosaccharides	188:239	Heparins, unfractionated heparin (UFH) and low molecular weight heparins (LMWHs), are heterogeneous mixtures of anticoagulant and non-anticoagulant oligosaccharides.
24310794	0	43	theme	heparins	66:73	arg1	oligosaccharides					46:61	pro- and anti-proliferative oligosaccharides	18:61	pro- and anti-proliferative oligosaccharides of heparins	18:73	Identification of pro- and anti-proliferative oligosaccharides of heparins.
24310794	6	44	theme	different	1056:1064	arg1	oligosaccharides					1066:1081	14 different oligosaccharides	1053:1081	14 different oligosaccharides of enoxaparin obtained through ion-exchange chromatography	1053:1140	The effects on cellular proliferation of 14 different oligosaccharides of enoxaparin obtained through ion-exchange chromatography were also assessed.
24310794	10	45	theme	preparation	1750:1760	arg1	methods					1762:1768	preparation methods	1750:1768	preparation methods	1750:1768	Our results indicate that slight differences in the composition of heparins' non-anticoagulant oligosaccharides, due to different origins of material and preparation methods, have the potential to cause diverse effects and highlight the need for additional characterisation of non-anticoagulant activities.
24310794	10	46	theme	activities	1891:1900	arg1	characterisation					1853:1868	additional characterisation	1842:1868	additional characterisation of non-anticoagulant activities	1842:1900	Our results indicate that slight differences in the composition of heparins' non-anticoagulant oligosaccharides, due to different origins of material and preparation methods, have the potential to cause diverse effects and highlight the need for additional characterisation of non-anticoagulant activities.
24310794	2	47	theme	non-anticoagulant	343:359	arg1	modulation					384:393	the modulation	380:393	the modulation of cellular growth	380:412	In addition to their well-known anticoagulant effect, heparins have shown to mediate a wide range of non-anticoagulant effects, including the modulation of cellular growth.
24310794	2	47	theme	non-anticoagulant	343:359	arg1	effects					361:367	non-anticoagulant effects	343:367	non-anticoagulant effects	343:367	In addition to their well-known anticoagulant effect, heparins have shown to mediate a wide range of non-anticoagulant effects, including the modulation of cellular growth.
24310794	10	48	from	differences	1629:1639	arg1	composition					1648:1658	the composition	1644:1658	the composition of heparins' non-anticoagulant oligosaccharides	1644:1706	Our results indicate that slight differences in the composition of heparins' non-anticoagulant oligosaccharides, due to different origins of material and preparation methods, have the potential to cause diverse effects and highlight the need for additional characterisation of non-anticoagulant activities.
24310794	10	49	theme	non-anticoagulant	1673:1689	arg1	oligosaccharides					1691:1706	non-anticoagulant oligosaccharides	1673:1706	heparins' non-anticoagulant oligosaccharides	1663:1706	Our results indicate that slight differences in the composition of heparins' non-anticoagulant oligosaccharides, due to different origins of material and preparation methods, have the potential to cause diverse effects and highlight the need for additional characterisation of non-anticoagulant activities.
24310794	10	50	contain	have	1771:1774	arg2	potential					1780:1788	the potential to cause diverse effects	1776:1813	the potential to cause diverse effects	1776:1813	Our results indicate that slight differences in the composition of heparins' non-anticoagulant oligosaccharides, due to different origins of material and preparation methods, have the potential to cause diverse effects and highlight the need for additional characterisation of non-anticoagulant activities.
24310794	10	50	contain	have	1771:1774	arg1	differences					1629:1639	slight differences	1622:1639	slight differences	1622:1639	Our results indicate that slight differences in the composition of heparins' non-anticoagulant oligosaccharides, due to different origins of material and preparation methods, have the potential to cause diverse effects and highlight the need for additional characterisation of non-anticoagulant activities.
24310794	1	51	theme	low	119:121	arg1	LMWHs					150:154	LMWHs	150:154	LMWHs	150:154	Heparins, unfractionated heparin (UFH) and low molecular weight heparins (LMWHs), are heterogeneous mixtures of anticoagulant and non-anticoagulant oligosaccharides.
24310794	1	51	theme	low	119:121	arg1	Heparins					76:83	Heparins	76:83	Heparins	76:83	Heparins, unfractionated heparin (UFH) and low molecular weight heparins (LMWHs), are heterogeneous mixtures of anticoagulant and non-anticoagulant oligosaccharides.
24310794	1	51	theme	low	119:121	arg1	heparins					140:147	low molecular weight heparins	119:147	low molecular weight heparins (LMWHs)	119:155	Heparins, unfractionated heparin (UFH) and low molecular weight heparins (LMWHs), are heterogeneous mixtures of anticoagulant and non-anticoagulant oligosaccharides.
24310794	4	52	dep	UFH	722:724	arg1	presence					710:717	presence	710:717	presence	710:717	This study investigated the proliferation of human colonic epithelial cancer cells in the presence of UFH and LMWHs (enoxaparin and dalteparin).
24310794	4	52	dep	UFH	722:724	arg1	the					706:708	the	706:708	the	706:708	This study investigated the proliferation of human colonic epithelial cancer cells in the presence of UFH and LMWHs (enoxaparin and dalteparin).
24310794	2	53	theme	anticoagulant	274:286	arg1	effect					288:293	their well-known anticoagulant effect	257:293	their well-known anticoagulant effect	257:293	In addition to their well-known anticoagulant effect, heparins have shown to mediate a wide range of non-anticoagulant effects, including the modulation of cellular growth.
24310794	8	54	theme	devoid	1366:1371	arg1	oligosaccharide					1350:1364	the smallest oligosaccharide	1337:1364	the smallest oligosaccharide devoid of any anticoagulant activity	1337:1401	Interestingly, the smallest oligosaccharide devoid of any anticoagulant activity showed the strongest anti-proliferative effect.
24310794	1	55	theme	molecular	123:131	arg1	LMWHs					150:154	LMWHs	150:154	LMWHs	150:154	Heparins, unfractionated heparin (UFH) and low molecular weight heparins (LMWHs), are heterogeneous mixtures of anticoagulant and non-anticoagulant oligosaccharides.
24310794	1	55	theme	molecular	123:131	arg1	Heparins					76:83	Heparins	76:83	Heparins	76:83	Heparins, unfractionated heparin (UFH) and low molecular weight heparins (LMWHs), are heterogeneous mixtures of anticoagulant and non-anticoagulant oligosaccharides.
24310794	1	55	theme	molecular	123:131	arg1	heparins					140:147	low molecular weight heparins	119:147	low molecular weight heparins (LMWHs)	119:155	Heparins, unfractionated heparin (UFH) and low molecular weight heparins (LMWHs), are heterogeneous mixtures of anticoagulant and non-anticoagulant oligosaccharides.
24310794	9	56	theme	anticoagulant	1520:1532	arg1	activity					1534:1541	their anticoagulant activity	1514:1541	their anticoagulant activity	1514:1541	Notably, heparins are currently standardised only according to their anticoagulant activity but not based on other non-anticoagulant properties.
24310794	1	57	theme	oligosaccharides	224:239	arg1	mixtures					176:183	heterogeneous mixtures	162:183	heterogeneous mixtures of anticoagulant and non-anticoagulant oligosaccharides	162:239	Heparins, unfractionated heparin (UFH) and low molecular weight heparins (LMWHs), are heterogeneous mixtures of anticoagulant and non-anticoagulant oligosaccharides.
24310794	1	57	theme	oligosaccharides	224:239	arg1	Heparins					76:83	Heparins	76:83	Heparins	76:83	Heparins, unfractionated heparin (UFH) and low molecular weight heparins (LMWHs), are heterogeneous mixtures of anticoagulant and non-anticoagulant oligosaccharides.
24310794	2	58	theme	well-known	263:272	arg1	effect					288:293	their well-known anticoagulant effect	257:293	their well-known anticoagulant effect	257:293	In addition to their well-known anticoagulant effect, heparins have shown to mediate a wide range of non-anticoagulant effects, including the modulation of cellular growth.
24310794	1	59	theme	weight	133:138	arg1	LMWHs					150:154	LMWHs	150:154	LMWHs	150:154	Heparins, unfractionated heparin (UFH) and low molecular weight heparins (LMWHs), are heterogeneous mixtures of anticoagulant and non-anticoagulant oligosaccharides.
24310794	1	59	theme	weight	133:138	arg1	Heparins					76:83	Heparins	76:83	Heparins	76:83	Heparins, unfractionated heparin (UFH) and low molecular weight heparins (LMWHs), are heterogeneous mixtures of anticoagulant and non-anticoagulant oligosaccharides.
24310794	1	59	theme	weight	133:138	arg1	heparins					140:147	low molecular weight heparins	119:147	low molecular weight heparins (LMWHs)	119:155	Heparins, unfractionated heparin (UFH) and low molecular weight heparins (LMWHs), are heterogeneous mixtures of anticoagulant and non-anticoagulant oligosaccharides.
24310794	8	60	theme	anti-proliferative	1424:1441	arg1	effect					1443:1448	the strongest anti-proliferative effect	1410:1448	the strongest anti-proliferative effect	1410:1448	Interestingly, the smallest oligosaccharide devoid of any anticoagulant activity showed the strongest anti-proliferative effect.
24310794	10	61	theme	different	1716:1724	arg1	origins					1726:1732	different origins	1716:1732	different origins of material and preparation methods	1716:1768	Our results indicate that slight differences in the composition of heparins' non-anticoagulant oligosaccharides, due to different origins of material and preparation methods, have the potential to cause diverse effects and highlight the need for additional characterisation of non-anticoagulant activities.
24310794	4	62	theme	cells	697:701	arg1	proliferation					648:660	the proliferation	644:660	the proliferation of human colonic epithelial cancer cells	644:701	This study investigated the proliferation of human colonic epithelial cancer cells in the presence of UFH and LMWHs (enoxaparin and dalteparin).
24310794	8	63	theme	anticoagulant	1380:1392	arg1	activity					1394:1401	any anticoagulant activity	1376:1401	any anticoagulant activity	1376:1401	Interestingly, the smallest oligosaccharide devoid of any anticoagulant activity showed the strongest anti-proliferative effect.
24310794	6	64	theme	enoxaparin	1086:1095	arg1	oligosaccharides					1066:1081	14 different oligosaccharides	1053:1081	14 different oligosaccharides of enoxaparin obtained through ion-exchange chromatography	1053:1140	The effects on cellular proliferation of 14 different oligosaccharides of enoxaparin obtained through ion-exchange chromatography were also assessed.
24310794	5	65	theme	arrest	916:921	arg1	induction					892:900	the induction	888:900	the induction of cell cycle arrest in the G₁ phase	888:937	In our experimental setting, all heparins caused a dose-dependent reduction in cellular growth, which correlated well with the induction of cell cycle arrest in the G₁ phase and which was not associated with significant changes in cell viability.
24310794	6	66	theme	ion-exchange	1114:1125	arg1	chromatography					1127:1140	ion-exchange chromatography	1114:1140	ion-exchange chromatography	1114:1140	The effects on cellular proliferation of 14 different oligosaccharides of enoxaparin obtained through ion-exchange chromatography were also assessed.
24310794	4	67	theme	colonic	671:677	arg1	cells					697:701	human colonic epithelial cancer cells	665:701	human colonic epithelial cancer cells	665:701	This study investigated the proliferation of human colonic epithelial cancer cells in the presence of UFH and LMWHs (enoxaparin and dalteparin).
24310794	10	68	dep	heparins	1663:1670	arg1	oligosaccharides					1691:1706	non-anticoagulant oligosaccharides	1673:1706	heparins' non-anticoagulant oligosaccharides	1663:1706	Our results indicate that slight differences in the composition of heparins' non-anticoagulant oligosaccharides, due to different origins of material and preparation methods, have the potential to cause diverse effects and highlight the need for additional characterisation of non-anticoagulant activities.
24310794	5	69	from	reduction	831:839	arg1	growth					853:858	cellular growth	844:858	cellular growth	844:858	In our experimental setting, all heparins caused a dose-dependent reduction in cellular growth, which correlated well with the induction of cell cycle arrest in the G₁ phase and which was not associated with significant changes in cell viability.
24310794	5	70	theme	dose-dependent	816:829	arg1	reduction					831:839	a dose-dependent reduction	814:839	a dose-dependent reduction	814:839	In our experimental setting, all heparins caused a dose-dependent reduction in cellular growth, which correlated well with the induction of cell cycle arrest in the G₁ phase and which was not associated with significant changes in cell viability.
24310794	5	71	from	induction	892:900	arg1	phase					933:937	the G₁ phase	926:937	the G₁ phase	926:937	In our experimental setting, all heparins caused a dose-dependent reduction in cellular growth, which correlated well with the induction of cell cycle arrest in the G₁ phase and which was not associated with significant changes in cell viability.
24310794	8	72	theme	smallest	1341:1348	arg1	oligosaccharide					1350:1364	the smallest oligosaccharide	1337:1364	the smallest oligosaccharide devoid of any anticoagulant activity	1337:1401	Interestingly, the smallest oligosaccharide devoid of any anticoagulant activity showed the strongest anti-proliferative effect.
24310794	4	73	theme	human	665:669	arg1	cells					697:701	human colonic epithelial cancer cells	665:701	human colonic epithelial cancer cells	665:701	This study investigated the proliferation of human colonic epithelial cancer cells in the presence of UFH and LMWHs (enoxaparin and dalteparin).
24342539	4	0	theme	g/Lh	828:831	arg1	productivity					807:818	a productivity	805:818	a productivity of 0.92 g/Lh	805:831	The optimal nutrient composition (18% jaggery, 3% DOJSC and 0.97% CSL) as suggested by the model resulted in production of 66.25 g/L pullulan with a productivity of 0.92 g/Lh.
24342539	2	1	from	effects	456:462	arg1	biosynthesis					515:526	pullulan biosynthesis	506:526	pullulan biosynthesis	506:526	A second order polynomial model was obtained using central composite design to understand the effects of interactions among these substrates on pullulan biosynthesis.
24342539	1	2	theme	feasible	169:176	arg1	process					178:184	an economically feasible process	153:184	an economically feasible process for the fermentative production of pullulan	153:228	Response surface methodology was used to develop an economically feasible process for the fermentative production of pullulan using agri-industrial residues, jaggery, de-oiled jatropha seed cake (DOJSC) and corn steep liquor (CSL), as sole media components.
24342539	1	3	theme	sole	339:342	arg1	residues					252:259	agri-industrial residues	236:259	agri-industrial residues	236:259	Response surface methodology was used to develop an economically feasible process for the fermentative production of pullulan using agri-industrial residues, jaggery, de-oiled jatropha seed cake (DOJSC) and corn steep liquor (CSL), as sole media components.
24342539	1	3	theme	sole	339:342	arg1	components					350:359	sole media components	339:359	sole media components	339:359	Response surface methodology was used to develop an economically feasible process for the fermentative production of pullulan using agri-industrial residues, jaggery, de-oiled jatropha seed cake (DOJSC) and corn steep liquor (CSL), as sole media components.
24342539	1	4	theme	de-oiled	271:278	arg1	residues					252:259	agri-industrial residues	236:259	agri-industrial residues	236:259	Response surface methodology was used to develop an economically feasible process for the fermentative production of pullulan using agri-industrial residues, jaggery, de-oiled jatropha seed cake (DOJSC) and corn steep liquor (CSL), as sole media components.
24342539	1	4	theme	de-oiled	271:278	arg1	DOJSC					300:304	DOJSC	300:304	DOJSC	300:304	Response surface methodology was used to develop an economically feasible process for the fermentative production of pullulan using agri-industrial residues, jaggery, de-oiled jatropha seed cake (DOJSC) and corn steep liquor (CSL), as sole media components.
24342539	1	4	theme	de-oiled	271:278	arg1	cake					294:297	de-oiled jatropha seed cake	271:297	de-oiled jatropha seed cake (DOJSC)	271:305	Response surface methodology was used to develop an economically feasible process for the fermentative production of pullulan using agri-industrial residues, jaggery, de-oiled jatropha seed cake (DOJSC) and corn steep liquor (CSL), as sole media components.
24342539	0	5	theme	surface	83:89	arg1	methodology					91:101	response surface methodology	74:101	response surface methodology	74:101	Cost effective production of pullulan from agri-industrial residues using response surface methodology.
24342539	5	6	theme	material	850:857	arg1	Analysis					834:841	Analysis	834:841	Analysis of raw material cost component for pullulan production	834:896	Analysis of raw material cost component for pullulan production suggested that sole utilization of agri-residues may lead to development of cost effective process for pullulan production.
24342539	5	7	theme	cost	859:862	arg1	component					864:872	cost component	859:872	raw material cost component for pullulan production	846:896	Analysis of raw material cost component for pullulan production suggested that sole utilization of agri-residues may lead to development of cost effective process for pullulan production.
24342539	1	8	theme	jatropha	280:287	arg1	residues					252:259	agri-industrial residues	236:259	agri-industrial residues	236:259	Response surface methodology was used to develop an economically feasible process for the fermentative production of pullulan using agri-industrial residues, jaggery, de-oiled jatropha seed cake (DOJSC) and corn steep liquor (CSL), as sole media components.
24342539	1	8	theme	jatropha	280:287	arg1	DOJSC					300:304	DOJSC	300:304	DOJSC	300:304	Response surface methodology was used to develop an economically feasible process for the fermentative production of pullulan using agri-industrial residues, jaggery, de-oiled jatropha seed cake (DOJSC) and corn steep liquor (CSL), as sole media components.
24342539	1	8	theme	jatropha	280:287	arg1	cake					294:297	de-oiled jatropha seed cake	271:297	de-oiled jatropha seed cake (DOJSC)	271:305	Response surface methodology was used to develop an economically feasible process for the fermentative production of pullulan using agri-industrial residues, jaggery, de-oiled jatropha seed cake (DOJSC) and corn steep liquor (CSL), as sole media components.
24342539	4	9	theme	nutrient	670:677	arg1	composition					679:689	The optimal nutrient composition	658:689	The optimal nutrient composition (18% jaggery, 3% DOJSC and 0.97% CSL) as suggested by the model	658:753	The optimal nutrient composition (18% jaggery, 3% DOJSC and 0.97% CSL) as suggested by the model resulted in production of 66.25 g/L pullulan with a productivity of 0.92 g/Lh.
24342539	1	10	theme	seed	289:292	arg1	residues					252:259	agri-industrial residues	236:259	agri-industrial residues	236:259	Response surface methodology was used to develop an economically feasible process for the fermentative production of pullulan using agri-industrial residues, jaggery, de-oiled jatropha seed cake (DOJSC) and corn steep liquor (CSL), as sole media components.
24342539	1	10	theme	seed	289:292	arg1	DOJSC					300:304	DOJSC	300:304	DOJSC	300:304	Response surface methodology was used to develop an economically feasible process for the fermentative production of pullulan using agri-industrial residues, jaggery, de-oiled jatropha seed cake (DOJSC) and corn steep liquor (CSL), as sole media components.
24342539	1	10	theme	seed	289:292	arg1	cake					294:297	de-oiled jatropha seed cake	271:297	de-oiled jatropha seed cake (DOJSC)	271:305	Response surface methodology was used to develop an economically feasible process for the fermentative production of pullulan using agri-industrial residues, jaggery, de-oiled jatropha seed cake (DOJSC) and corn steep liquor (CSL), as sole media components.
24342539	5	11	dep	material	850:857	arg1	component					864:872	cost component	859:872	raw material cost component for pullulan production	846:896	Analysis of raw material cost component for pullulan production suggested that sole utilization of agri-residues may lead to development of cost effective process for pullulan production.
24342539	0	12	from	residues	59:66	arg1	production					15:24	Cost effective production	0:24	Cost effective production of pullulan from agri-industrial residues using response surface methodology.	0:102	Cost effective production of pullulan from agri-industrial residues using response surface methodology.
24342539	3	13	theme	lower	553:557	arg1	concentrations					559:572	lower concentrations	553:572	lower concentrations of CSL and DOJSC	553:589	Results indicated that, lower concentrations of CSL and DOJSC and higher concentrations of jaggery favoured pullulan production.
24342539	5	14	theme	agri-residues	933:945	arg1	utilization					918:928	sole utilization	913:928	sole utilization of agri-residues	913:945	Analysis of raw material cost component for pullulan production suggested that sole utilization of agri-residues may lead to development of cost effective process for pullulan production.
24342539	4	15	dep	%	706:706	arg1	DOJSC					708:712	DOJSC	708:712	3% DOJSC	705:712	The optimal nutrient composition (18% jaggery, 3% DOJSC and 0.97% CSL) as suggested by the model resulted in production of 66.25 g/L pullulan with a productivity of 0.92 g/Lh.
24342539	2	16	theme	pullulan	506:513	arg1	biosynthesis					515:526	pullulan biosynthesis	506:526	pullulan biosynthesis	506:526	A second order polynomial model was obtained using central composite design to understand the effects of interactions among these substrates on pullulan biosynthesis.
24342539	1	17	theme	media	344:348	arg1	residues					252:259	agri-industrial residues	236:259	agri-industrial residues	236:259	Response surface methodology was used to develop an economically feasible process for the fermentative production of pullulan using agri-industrial residues, jaggery, de-oiled jatropha seed cake (DOJSC) and corn steep liquor (CSL), as sole media components.
24342539	1	17	theme	media	344:348	arg1	components					350:359	sole media components	339:359	sole media components	339:359	Response surface methodology was used to develop an economically feasible process for the fermentative production of pullulan using agri-industrial residues, jaggery, de-oiled jatropha seed cake (DOJSC) and corn steep liquor (CSL), as sole media components.
24342539	4	18	dep	%	694:694	arg1	jaggery					696:702	jaggery	696:702	18% jaggery	692:702	The optimal nutrient composition (18% jaggery, 3% DOJSC and 0.97% CSL) as suggested by the model resulted in production of 66.25 g/L pullulan with a productivity of 0.92 g/Lh.
24342539	2	19	theme	second	364:369	arg1	model					388:392	A second order polynomial model	362:392	A second order polynomial model	362:392	A second order polynomial model was obtained using central composite design to understand the effects of interactions among these substrates on pullulan biosynthesis.
24342539	2	20	theme	polynomial	377:386	arg1	model					388:392	A second order polynomial model	362:392	A second order polynomial model	362:392	A second order polynomial model was obtained using central composite design to understand the effects of interactions among these substrates on pullulan biosynthesis.
24342539	2	21	theme	order	371:375	arg1	model					388:392	A second order polynomial model	362:392	A second order polynomial model	362:392	A second order polynomial model was obtained using central composite design to understand the effects of interactions among these substrates on pullulan biosynthesis.
24342539	0	22	theme	effective	5:13	arg1	production					15:24	Cost effective production	0:24	Cost effective production of pullulan from agri-industrial residues using response surface methodology.	0:102	Cost effective production of pullulan from agri-industrial residues using response surface methodology.
24342539	2	23	theme	interactions	467:478	arg1	effects					456:462	the effects	452:462	the effects of interactions among these substrates on pullulan biosynthesis	452:526	A second order polynomial model was obtained using central composite design to understand the effects of interactions among these substrates on pullulan biosynthesis.
24342539	5	24	theme	sole	913:916	arg1	utilization					918:928	sole utilization	913:928	sole utilization of agri-residues	913:945	Analysis of raw material cost component for pullulan production suggested that sole utilization of agri-residues may lead to development of cost effective process for pullulan production.
24342539	1	25	theme	fermentative	194:205	arg1	production					207:216	the fermentative production	190:216	the fermentative production of pullulan	190:228	Response surface methodology was used to develop an economically feasible process for the fermentative production of pullulan using agri-industrial residues, jaggery, de-oiled jatropha seed cake (DOJSC) and corn steep liquor (CSL), as sole media components.
24342539	1	26	theme	Response	104:111	arg1	methodology					121:131	Response surface methodology	104:131	Response surface methodology	104:131	Response surface methodology was used to develop an economically feasible process for the fermentative production of pullulan using agri-industrial residues, jaggery, de-oiled jatropha seed cake (DOJSC) and corn steep liquor (CSL), as sole media components.
24342539	2	27	theme	composite	421:429	arg1	design					431:436	central composite design	413:436	central composite design	413:436	A second order polynomial model was obtained using central composite design to understand the effects of interactions among these substrates on pullulan biosynthesis.
24342539	4	28	theme	g/L	787:789	arg1	pullulan					791:798	66.25 g/L pullulan	781:798	66.25 g/L pullulan with a productivity of 0.92 g/Lh	781:831	The optimal nutrient composition (18% jaggery, 3% DOJSC and 0.97% CSL) as suggested by the model resulted in production of 66.25 g/L pullulan with a productivity of 0.92 g/Lh.
24342539	1	29	theme	surface	113:119	arg1	methodology					121:131	Response surface methodology	104:131	Response surface methodology	104:131	Response surface methodology was used to develop an economically feasible process for the fermentative production of pullulan using agri-industrial residues, jaggery, de-oiled jatropha seed cake (DOJSC) and corn steep liquor (CSL), as sole media components.
24342539	5	30	theme	effective	979:987	arg1	process					989:995	cost effective process	974:995	cost effective process for pullulan production	974:1019	Analysis of raw material cost component for pullulan production suggested that sole utilization of agri-residues may lead to development of cost effective process for pullulan production.
24342539	2	31	theme	central	413:419	arg1	design					431:436	central composite design	413:436	central composite design	413:436	A second order polynomial model was obtained using central composite design to understand the effects of interactions among these substrates on pullulan biosynthesis.
24342539	1	32	theme	pullulan	221:228	arg1	production					207:216	the fermentative production	190:216	the fermentative production of pullulan	190:228	Response surface methodology was used to develop an economically feasible process for the fermentative production of pullulan using agri-industrial residues, jaggery, de-oiled jatropha seed cake (DOJSC) and corn steep liquor (CSL), as sole media components.
24342539	4	33	theme	66.25	781:785	arg1	pullulan					791:798	66.25 g/L pullulan	781:798	66.25 g/L pullulan with a productivity of 0.92 g/Lh	781:831	The optimal nutrient composition (18% jaggery, 3% DOJSC and 0.97% CSL) as suggested by the model resulted in production of 66.25 g/L pullulan with a productivity of 0.92 g/Lh.
24342539	0	34	theme	pullulan	29:36	arg1	production					15:24	Cost effective production	0:24	Cost effective production of pullulan from agri-industrial residues using response surface methodology.	0:102	Cost effective production of pullulan from agri-industrial residues using response surface methodology.
24342539	1	35	theme	corn	311:314	arg1	CSL					330:332	CSL	330:332	CSL	330:332	Response surface methodology was used to develop an economically feasible process for the fermentative production of pullulan using agri-industrial residues, jaggery, de-oiled jatropha seed cake (DOJSC) and corn steep liquor (CSL), as sole media components.
24342539	1	35	theme	corn	311:314	arg1	liquor					322:327	corn steep liquor	311:327	corn steep liquor (CSL)	311:333	Response surface methodology was used to develop an economically feasible process for the fermentative production of pullulan using agri-industrial residues, jaggery, de-oiled jatropha seed cake (DOJSC) and corn steep liquor (CSL), as sole media components.
24342539	1	35	theme	corn	311:314	arg1	residues					252:259	agri-industrial residues	236:259	agri-industrial residues	236:259	Response surface methodology was used to develop an economically feasible process for the fermentative production of pullulan using agri-industrial residues, jaggery, de-oiled jatropha seed cake (DOJSC) and corn steep liquor (CSL), as sole media components.
24342539	3	36	theme	pullulan	637:644	arg1	production					646:655	pullulan production	637:655	pullulan production	637:655	Results indicated that, lower concentrations of CSL and DOJSC and higher concentrations of jaggery favoured pullulan production.
24342539	3	37	theme	higher	595:600	arg1	concentrations					602:615	higher concentrations	595:615	higher concentrations of jaggery	595:626	Results indicated that, lower concentrations of CSL and DOJSC and higher concentrations of jaggery favoured pullulan production.
24342539	1	38	theme	agri-industrial	236:250	arg1	jaggery					262:268	jaggery	262:268	jaggery	262:268	Response surface methodology was used to develop an economically feasible process for the fermentative production of pullulan using agri-industrial residues, jaggery, de-oiled jatropha seed cake (DOJSC) and corn steep liquor (CSL), as sole media components.
24342539	1	38	theme	agri-industrial	236:250	arg1	components					350:359	sole media components	339:359	sole media components	339:359	Response surface methodology was used to develop an economically feasible process for the fermentative production of pullulan using agri-industrial residues, jaggery, de-oiled jatropha seed cake (DOJSC) and corn steep liquor (CSL), as sole media components.
24342539	1	38	theme	agri-industrial	236:250	arg1	residues					252:259	agri-industrial residues	236:259	agri-industrial residues	236:259	Response surface methodology was used to develop an economically feasible process for the fermentative production of pullulan using agri-industrial residues, jaggery, de-oiled jatropha seed cake (DOJSC) and corn steep liquor (CSL), as sole media components.
24342539	1	38	theme	agri-industrial	236:250	arg1	liquor					322:327	corn steep liquor	311:327	corn steep liquor (CSL)	311:333	Response surface methodology was used to develop an economically feasible process for the fermentative production of pullulan using agri-industrial residues, jaggery, de-oiled jatropha seed cake (DOJSC) and corn steep liquor (CSL), as sole media components.
24342539	1	38	theme	agri-industrial	236:250	arg1	cake					294:297	de-oiled jatropha seed cake	271:297	de-oiled jatropha seed cake (DOJSC)	271:305	Response surface methodology was used to develop an economically feasible process for the fermentative production of pullulan using agri-industrial residues, jaggery, de-oiled jatropha seed cake (DOJSC) and corn steep liquor (CSL), as sole media components.
24342539	1	39	theme	steep	316:320	arg1	CSL					330:332	CSL	330:332	CSL	330:332	Response surface methodology was used to develop an economically feasible process for the fermentative production of pullulan using agri-industrial residues, jaggery, de-oiled jatropha seed cake (DOJSC) and corn steep liquor (CSL), as sole media components.
24342539	1	39	theme	steep	316:320	arg1	liquor					322:327	corn steep liquor	311:327	corn steep liquor (CSL)	311:333	Response surface methodology was used to develop an economically feasible process for the fermentative production of pullulan using agri-industrial residues, jaggery, de-oiled jatropha seed cake (DOJSC) and corn steep liquor (CSL), as sole media components.
24342539	1	39	theme	steep	316:320	arg1	residues					252:259	agri-industrial residues	236:259	agri-industrial residues	236:259	Response surface methodology was used to develop an economically feasible process for the fermentative production of pullulan using agri-industrial residues, jaggery, de-oiled jatropha seed cake (DOJSC) and corn steep liquor (CSL), as sole media components.
24342539	4	40	theme	pullulan	791:798	arg1	production					767:776	production	767:776	production of 66.25 g/L pullulan with a productivity of 0.92 g/Lh	767:831	The optimal nutrient composition (18% jaggery, 3% DOJSC and 0.97% CSL) as suggested by the model resulted in production of 66.25 g/L pullulan with a productivity of 0.92 g/Lh.
24342539	0	41	theme	agri-industrial	43:57	arg1	residues					59:66	agri-industrial residues	43:66	agri-industrial residues using response surface methodology	43:101	Cost effective production of pullulan from agri-industrial residues using response surface methodology.
24342539	5	42	theme	pullulan	1001:1008	arg1	production					1010:1019	pullulan production	1001:1019	pullulan production	1001:1019	Analysis of raw material cost component for pullulan production suggested that sole utilization of agri-residues may lead to development of cost effective process for pullulan production.
24342539	3	43	theme	DOJSC	585:589	arg1	concentrations					559:572	lower concentrations	553:572	lower concentrations of CSL and DOJSC	553:589	Results indicated that, lower concentrations of CSL and DOJSC and higher concentrations of jaggery favoured pullulan production.
24342539	3	43	theme	DOJSC	585:589	arg1	concentrations					602:615	higher concentrations	595:615	higher concentrations of jaggery	595:626	Results indicated that, lower concentrations of CSL and DOJSC and higher concentrations of jaggery favoured pullulan production.
24342539	3	44	theme	jaggery	620:626	arg1	concentrations					559:572	lower concentrations	553:572	lower concentrations of CSL and DOJSC	553:589	Results indicated that, lower concentrations of CSL and DOJSC and higher concentrations of jaggery favoured pullulan production.
24342539	3	44	theme	jaggery	620:626	arg1	concentrations					602:615	higher concentrations	595:615	higher concentrations of jaggery	595:626	Results indicated that, lower concentrations of CSL and DOJSC and higher concentrations of jaggery favoured pullulan production.
24342539	5	45	theme	process	989:995	arg1	development					959:969	development	959:969	development of cost effective process for pullulan production	959:1019	Analysis of raw material cost component for pullulan production suggested that sole utilization of agri-residues may lead to development of cost effective process for pullulan production.
24342539	4	46	dep	%	722:722	arg1	CSL					724:726	CSL	724:726	0.97% CSL	718:726	The optimal nutrient composition (18% jaggery, 3% DOJSC and 0.97% CSL) as suggested by the model resulted in production of 66.25 g/L pullulan with a productivity of 0.92 g/Lh.
24342539	1	47	used	used	137:140	arg2	methodology					121:131	Response surface methodology	104:131	Response surface methodology	104:131	Response surface methodology was used to develop an economically feasible process for the fermentative production of pullulan using agri-industrial residues, jaggery, de-oiled jatropha seed cake (DOJSC) and corn steep liquor (CSL), as sole media components.
24342539	4	48	with	pullulan	791:798	arg1	productivity					807:818	a productivity	805:818	a productivity of 0.92 g/Lh	805:831	The optimal nutrient composition (18% jaggery, 3% DOJSC and 0.97% CSL) as suggested by the model resulted in production of 66.25 g/L pullulan with a productivity of 0.92 g/Lh.
24342539	4	49	dep	composition	679:689	arg1	suggested					732:740	suggested	732:740	suggested by the model	732:753	The optimal nutrient composition (18% jaggery, 3% DOJSC and 0.97% CSL) as suggested by the model resulted in production of 66.25 g/L pullulan with a productivity of 0.92 g/Lh.
24342539	4	49	dep	composition	679:689	arg1	%					722:722	0.97%	718:722	0.97% CSL	718:726	The optimal nutrient composition (18% jaggery, 3% DOJSC and 0.97% CSL) as suggested by the model resulted in production of 66.25 g/L pullulan with a productivity of 0.92 g/Lh.
24342539	4	49	dep	composition	679:689	arg1	%					694:694	18%	692:694	18% jaggery	692:702	The optimal nutrient composition (18% jaggery, 3% DOJSC and 0.97% CSL) as suggested by the model resulted in production of 66.25 g/L pullulan with a productivity of 0.92 g/Lh.
24342539	4	49	dep	composition	679:689	arg1	%					706:706	3%	705:706	3% DOJSC	705:712	The optimal nutrient composition (18% jaggery, 3% DOJSC and 0.97% CSL) as suggested by the model resulted in production of 66.25 g/L pullulan with a productivity of 0.92 g/Lh.
24342539	5	50	theme	raw	846:848	arg1	material					850:857	raw material	846:857	raw material cost component for pullulan production	846:896	Analysis of raw material cost component for pullulan production suggested that sole utilization of agri-residues may lead to development of cost effective process for pullulan production.
24342539	5	51	theme	pullulan	878:885	arg1	production					887:896	pullulan production	878:896	pullulan production	878:896	Analysis of raw material cost component for pullulan production suggested that sole utilization of agri-residues may lead to development of cost effective process for pullulan production.
24342539	0	52	theme	response	74:81	arg1	methodology					91:101	response surface methodology	74:101	response surface methodology	74:101	Cost effective production of pullulan from agri-industrial residues using response surface methodology.
24342539	4	53	theme	optimal	662:668	arg1	composition					679:689	The optimal nutrient composition	658:689	The optimal nutrient composition (18% jaggery, 3% DOJSC and 0.97% CSL) as suggested by the model	658:753	The optimal nutrient composition (18% jaggery, 3% DOJSC and 0.97% CSL) as suggested by the model resulted in production of 66.25 g/L pullulan with a productivity of 0.92 g/Lh.
24342539	3	54	theme	CSL	577:579	arg1	concentrations					559:572	lower concentrations	553:572	lower concentrations of CSL and DOJSC	553:589	Results indicated that, lower concentrations of CSL and DOJSC and higher concentrations of jaggery favoured pullulan production.
24342539	3	54	theme	CSL	577:579	arg1	concentrations					602:615	higher concentrations	595:615	higher concentrations of jaggery	595:626	Results indicated that, lower concentrations of CSL and DOJSC and higher concentrations of jaggery favoured pullulan production.
25246061	2	0	from	strains	496:502	arg1	polysaccharides					459:473	Extracellular polysaccharides	445:473	Extracellular polysaccharides from three different strains of Pleurotus tuber-regium	445:528	Extracellular polysaccharides from three different strains of Pleurotus tuber-regium were extracted and labeled as HP (high-percentage), MP (medium-percentage) and LP (low-percentage).
25246061	2	0	from	strains	496:502	arg1	LP					609:610	LP	609:610	LP (low-percentage)	609:627	Extracellular polysaccharides from three different strains of Pleurotus tuber-regium were extracted and labeled as HP (high-percentage), MP (medium-percentage) and LP (low-percentage).
25246061	2	0	from	strains	496:502	arg1	MP					582:583	MP	582:583	MP (medium-percentage)	582:603	Extracellular polysaccharides from three different strains of Pleurotus tuber-regium were extracted and labeled as HP (high-percentage), MP (medium-percentage) and LP (low-percentage).
25246061	2	0	from	strains	496:502	arg1	HP					560:561	HP	560:561	HP (high-percentage)	560:579	Extracellular polysaccharides from three different strains of Pleurotus tuber-regium were extracted and labeled as HP (high-percentage), MP (medium-percentage) and LP (low-percentage).
25246061	6	1	theme	reduced	1123:1129	arg1	PUFA					1137:1140	reduced MUFA/ PUFA	1123:1140	reduced MUFA/ PUFA	1123:1140	Increased fatty acid component n-6/n-3 ratio in liver and plasma of obese-diabetic rats was attenuated, while, reduced MUFA/ PUFA and MUFA/SFA ratios were restored (P < 0.01) with polysaccharides treatment.
25246061	6	2	theme	polysaccharides	1192:1206	arg1	treatment					1208:1216	polysaccharides treatment	1192:1216	polysaccharides treatment	1192:1216	Increased fatty acid component n-6/n-3 ratio in liver and plasma of obese-diabetic rats was attenuated, while, reduced MUFA/ PUFA and MUFA/SFA ratios were restored (P < 0.01) with polysaccharides treatment.
25246061	9	3	theme	hypolipidemic	1735:1747	arg1	properties					1749:1758	its hypoglycemic and hypolipidemic properties	1714:1758	its hypoglycemic and hypolipidemic properties	1714:1758	These findings concluded that stable fatty acid components and activated PPAR-α by polysaccharides may contribute to its hypoglycemic and hypolipidemic properties.
25246061	10	4	theme	nutritional	1811:1821	arg1	tuber-regium					1775:1786	P. tuber-regium	1772:1786	P. tuber-regium	1772:1786	Therefore, P. tuber-regium could be considered as nutritional supplement to treat diabetic complications.
25246061	10	4	theme	nutritional	1811:1821	arg1	supplement					1823:1832	nutritional supplement	1811:1832	nutritional supplement	1811:1832	Therefore, P. tuber-regium could be considered as nutritional supplement to treat diabetic complications.
25246061	1	5	theme	polysaccharides	186:200	arg1	effect					153:158	the effect	149:158	the effect of mushroom extracellular polysaccharides on fatty acid composition and liver peroxisome proliferator-activated receptor-alpha (PPAR-α) expression in obese-diabetic rats	149:328	In this study, the effect of mushroom extracellular polysaccharides on fatty acid composition and liver peroxisome proliferator-activated receptor-alpha (PPAR-α) expression in obese-diabetic rats was investigated, and distinguished the association among anti-obesity, hypoglycemic and hypolipidemic properties.
25246061	7	6	theme	polysaccharides	1438:1452	arg1	supplementation					1454:1468	polysaccharides supplementation	1438:1468	polysaccharides supplementation	1438:1468	Furthermore, elevated serum total cholesterol, triglycerides and low-density lipoprotein (LDL) concentrations were controlled, and parallel restoration of decreased high-density lipoprotein (HDL) levels were found with polysaccharides supplementation.
25246061	1	7	theme	fatty	205:209	arg1	composition					216:226	fatty acid composition	205:226	fatty acid composition	205:226	In this study, the effect of mushroom extracellular polysaccharides on fatty acid composition and liver peroxisome proliferator-activated receptor-alpha (PPAR-α) expression in obese-diabetic rats was investigated, and distinguished the association among anti-obesity, hypoglycemic and hypolipidemic properties.
25246061	10	8	theme	diabetic	1843:1850	arg1	complications					1852:1864	diabetic complications	1843:1864	diabetic complications	1843:1864	Therefore, P. tuber-regium could be considered as nutritional supplement to treat diabetic complications.
25246061	7	9	theme	levels	1415:1420	arg1	restoration					1359:1369	parallel restoration	1350:1369	parallel restoration of decreased high-density lipoprotein (HDL) levels	1350:1420	Furthermore, elevated serum total cholesterol, triglycerides and low-density lipoprotein (LDL) concentrations were controlled, and parallel restoration of decreased high-density lipoprotein (HDL) levels were found with polysaccharides supplementation.
25246061	1	10	theme	liver	232:236	arg1	receptor-alpha					272:285	liver peroxisome proliferator-activated receptor-alpha	232:285	liver peroxisome proliferator-activated receptor-alpha (PPAR-α) expression in obese-diabetic rats	232:328	In this study, the effect of mushroom extracellular polysaccharides on fatty acid composition and liver peroxisome proliferator-activated receptor-alpha (PPAR-α) expression in obese-diabetic rats was investigated, and distinguished the association among anti-obesity, hypoglycemic and hypolipidemic properties.
25246061	1	10	theme	liver	232:236	arg1	PPAR-α					288:293	PPAR-α	288:293	PPAR-α	288:293	In this study, the effect of mushroom extracellular polysaccharides on fatty acid composition and liver peroxisome proliferator-activated receptor-alpha (PPAR-α) expression in obese-diabetic rats was investigated, and distinguished the association among anti-obesity, hypoglycemic and hypolipidemic properties.
25246061	0	11	from	progression	72:82	arg1	rats					128:131	obese-diabetic rats	113:131	obese-diabetic rats	113:131	Effect of Pleurotus tuber-regium polysaccharides supplementation on the progression of diabetes complications in obese-diabetic rats.
25246061	8	12	theme	up-regulated	1524:1535	arg1	expression					1555:1564	up-regulated liver PPAR-α mRNA expression	1524:1564	up-regulated liver PPAR-α mRNA expression	1524:1564	This hypolipidemic property might be associated with up-regulated liver PPAR-α mRNA expression and protein levels (P < 0.01).
25246061	7	13	theme	lipoprotein	1397:1407	arg1	levels					1415:1420	decreased high-density lipoprotein (HDL) levels	1374:1420	decreased high-density lipoprotein (HDL) levels	1374:1420	Furthermore, elevated serum total cholesterol, triglycerides and low-density lipoprotein (LDL) concentrations were controlled, and parallel restoration of decreased high-density lipoprotein (HDL) levels were found with polysaccharides supplementation.
25246061	3	14	theme	high-fat	674:681	arg1	diet					683:686	chronic high-fat diet	666:686	chronic high-fat diet	666:686	Obese- diabetes (OD) was induced by chronic high-fat diet plus streptozotocin (STZ) injections.
25246061	5	15	theme	STZ-induced	939:949	arg1	hyperglycemia					951:963	STZ-induced hyperglycemia	939:963	STZ-induced hyperglycemia	939:963	High-fat diet plus STZ-induced hyperglycemia was prominently attenuated by polysaccharides.
25246061	8	16	theme	PPAR-α	1543:1548	arg1	expression					1555:1564	up-regulated liver PPAR-α mRNA expression	1524:1564	up-regulated liver PPAR-α mRNA expression	1524:1564	This hypolipidemic property might be associated with up-regulated liver PPAR-α mRNA expression and protein levels (P < 0.01).
25246061	7	17	theme	decreased	1374:1382	arg1	levels					1415:1420	decreased high-density lipoprotein (HDL) levels	1374:1420	decreased high-density lipoprotein (HDL) levels	1374:1420	Furthermore, elevated serum total cholesterol, triglycerides and low-density lipoprotein (LDL) concentrations were controlled, and parallel restoration of decreased high-density lipoprotein (HDL) levels were found with polysaccharides supplementation.
25246061	7	18	theme	cholesterol	1253:1263	arg1	concentrations					1314:1327	elevated serum total cholesterol, triglycerides and low-density lipoprotein (LDL) concentrations	1232:1327	elevated serum total cholesterol, triglycerides and low-density lipoprotein (LDL) concentrations	1232:1327	Furthermore, elevated serum total cholesterol, triglycerides and low-density lipoprotein (LDL) concentrations were controlled, and parallel restoration of decreased high-density lipoprotein (HDL) levels were found with polysaccharides supplementation.
25246061	0	19	theme	diabetes	87:94	arg1	complications					96:108	diabetes complications	87:108	diabetes complications	87:108	Effect of Pleurotus tuber-regium polysaccharides supplementation on the progression of diabetes complications in obese-diabetic rats.
25246061	1	20	theme	anti-obesity	388:399	arg1	properties					433:442	anti-obesity, hypoglycemic and hypolipidemic properties	388:442	anti-obesity, hypoglycemic and hypolipidemic properties	388:442	In this study, the effect of mushroom extracellular polysaccharides on fatty acid composition and liver peroxisome proliferator-activated receptor-alpha (PPAR-α) expression in obese-diabetic rats was investigated, and distinguished the association among anti-obesity, hypoglycemic and hypolipidemic properties.
25246061	1	21	theme	mushroom	163:170	arg1	polysaccharides					186:200	mushroom extracellular polysaccharides	163:200	mushroom extracellular polysaccharides	163:200	In this study, the effect of mushroom extracellular polysaccharides on fatty acid composition and liver peroxisome proliferator-activated receptor-alpha (PPAR-α) expression in obese-diabetic rats was investigated, and distinguished the association among anti-obesity, hypoglycemic and hypolipidemic properties.
25246061	6	22	theme	component	1033:1041	arg1	ratio					1051:1055	Increased fatty acid component n-6/n-3 ratio	1012:1055	Increased fatty acid component n-6/n-3 ratio in liver and plasma of obese-diabetic rats	1012:1098	Increased fatty acid component n-6/n-3 ratio in liver and plasma of obese-diabetic rats was attenuated, while, reduced MUFA/ PUFA and MUFA/SFA ratios were restored (P < 0.01) with polysaccharides treatment.
25246061	0	23	from	Effect	0:5	arg1	progression					72:82	the progression	68:82	the progression of diabetes complications in obese-diabetic rats	68:131	Effect of Pleurotus tuber-regium polysaccharides supplementation on the progression of diabetes complications in obese-diabetic rats.
25246061	1	24	theme	hypoglycemic	402:413	arg1	properties					433:442	anti-obesity, hypoglycemic and hypolipidemic properties	388:442	anti-obesity, hypoglycemic and hypolipidemic properties	388:442	In this study, the effect of mushroom extracellular polysaccharides on fatty acid composition and liver peroxisome proliferator-activated receptor-alpha (PPAR-α) expression in obese-diabetic rats was investigated, and distinguished the association among anti-obesity, hypoglycemic and hypolipidemic properties.
25246061	6	25	theme	fatty	1022:1026	arg1	ratio					1051:1055	Increased fatty acid component n-6/n-3 ratio	1012:1055	Increased fatty acid component n-6/n-3 ratio in liver and plasma of obese-diabetic rats	1012:1098	Increased fatty acid component n-6/n-3 ratio in liver and plasma of obese-diabetic rats was attenuated, while, reduced MUFA/ PUFA and MUFA/SFA ratios were restored (P < 0.01) with polysaccharides treatment.
25246061	4	26	theme	body	818:821	arg1	weight/8-week					823:835	20 mg/kg body weight/8-week	809:835	20 mg/kg body weight/8-week	809:835	Simultaneously to the diet, polysaccharides were orally administered to OD groups (20 mg/kg body weight/8-week), and categorized into OD+HP, OD+MP and OD+LP groups (n = 10/group), respectively.
25246061	4	26	theme	body	818:821	arg1	groups					801:806	OD groups	798:806	OD groups (20 mg/kg body weight/8-week)	798:836	Simultaneously to the diet, polysaccharides were orally administered to OD groups (20 mg/kg body weight/8-week), and categorized into OD+HP, OD+MP and OD+LP groups (n = 10/group), respectively.
25246061	1	27	theme	hypolipidemic	419:431	arg1	properties					433:442	anti-obesity, hypoglycemic and hypolipidemic properties	388:442	anti-obesity, hypoglycemic and hypolipidemic properties	388:442	In this study, the effect of mushroom extracellular polysaccharides on fatty acid composition and liver peroxisome proliferator-activated receptor-alpha (PPAR-α) expression in obese-diabetic rats was investigated, and distinguished the association among anti-obesity, hypoglycemic and hypolipidemic properties.
25246061	2	28	theme	Extracellular	445:457	arg1	polysaccharides					459:473	Extracellular polysaccharides	445:473	Extracellular polysaccharides from three different strains of Pleurotus tuber-regium	445:528	Extracellular polysaccharides from three different strains of Pleurotus tuber-regium were extracted and labeled as HP (high-percentage), MP (medium-percentage) and LP (low-percentage).
25246061	2	28	theme	Extracellular	445:457	arg1	LP					609:610	LP	609:610	LP (low-percentage)	609:627	Extracellular polysaccharides from three different strains of Pleurotus tuber-regium were extracted and labeled as HP (high-percentage), MP (medium-percentage) and LP (low-percentage).
25246061	2	28	theme	Extracellular	445:457	arg1	MP					582:583	MP	582:583	MP (medium-percentage)	582:603	Extracellular polysaccharides from three different strains of Pleurotus tuber-regium were extracted and labeled as HP (high-percentage), MP (medium-percentage) and LP (low-percentage).
25246061	2	28	theme	Extracellular	445:457	arg1	HP					560:561	HP	560:561	HP (high-percentage)	560:579	Extracellular polysaccharides from three different strains of Pleurotus tuber-regium were extracted and labeled as HP (high-percentage), MP (medium-percentage) and LP (low-percentage).
25246061	1	29	theme	obese-diabetic	310:323	arg1	rats					325:328	obese-diabetic rats	310:328	obese-diabetic rats	310:328	In this study, the effect of mushroom extracellular polysaccharides on fatty acid composition and liver peroxisome proliferator-activated receptor-alpha (PPAR-α) expression in obese-diabetic rats was investigated, and distinguished the association among anti-obesity, hypoglycemic and hypolipidemic properties.
25246061	7	30	theme	parallel	1350:1357	arg1	restoration					1359:1369	parallel restoration	1350:1369	parallel restoration of decreased high-density lipoprotein (HDL) levels	1350:1420	Furthermore, elevated serum total cholesterol, triglycerides and low-density lipoprotein (LDL) concentrations were controlled, and parallel restoration of decreased high-density lipoprotein (HDL) levels were found with polysaccharides supplementation.
25246061	2	31	theme	tuber-regium	517:528	arg1	strains					496:502	three different strains	480:502	three different strains of Pleurotus tuber-regium	480:528	Extracellular polysaccharides from three different strains of Pleurotus tuber-regium were extracted and labeled as HP (high-percentage), MP (medium-percentage) and LP (low-percentage).
25246061	0	32	theme	Pleurotus	10:18	arg1	supplementation					49:63	Pleurotus tuber-regium polysaccharides supplementation	10:63	Pleurotus tuber-regium polysaccharides supplementation	10:63	Effect of Pleurotus tuber-regium polysaccharides supplementation on the progression of diabetes complications in obese-diabetic rats.
25246061	9	33	theme	fatty	1634:1638	arg1	components					1645:1654	stable fatty acid components	1627:1654	stable fatty acid components	1627:1654	These findings concluded that stable fatty acid components and activated PPAR-α by polysaccharides may contribute to its hypoglycemic and hypolipidemic properties.
25246061	6	34	theme	obese-diabetic	1080:1093	arg1	rats					1095:1098	obese-diabetic rats	1080:1098	obese-diabetic rats	1080:1098	Increased fatty acid component n-6/n-3 ratio in liver and plasma of obese-diabetic rats was attenuated, while, reduced MUFA/ PUFA and MUFA/SFA ratios were restored (P < 0.01) with polysaccharides treatment.
25246061	2	35	theme	different	486:494	arg1	strains					496:502	three different strains	480:502	three different strains of Pleurotus tuber-regium	480:528	Extracellular polysaccharides from three different strains of Pleurotus tuber-regium were extracted and labeled as HP (high-percentage), MP (medium-percentage) and LP (low-percentage).
25246061	0	36	theme	polysaccharides	33:47	arg1	supplementation					49:63	Pleurotus tuber-regium polysaccharides supplementation	10:63	Pleurotus tuber-regium polysaccharides supplementation	10:63	Effect of Pleurotus tuber-regium polysaccharides supplementation on the progression of diabetes complications in obese-diabetic rats.
25246061	9	37	theme	activated	1660:1668	arg1	PPAR-α					1670:1675	activated PPAR-α	1660:1675	activated PPAR-α by polysaccharides	1660:1694	These findings concluded that stable fatty acid components and activated PPAR-α by polysaccharides may contribute to its hypoglycemic and hypolipidemic properties.
25246061	9	38	theme	hypoglycemic	1718:1729	arg1	properties					1749:1758	its hypoglycemic and hypolipidemic properties	1714:1758	its hypoglycemic and hypolipidemic properties	1714:1758	These findings concluded that stable fatty acid components and activated PPAR-α by polysaccharides may contribute to its hypoglycemic and hypolipidemic properties.
25246061	1	39	theme	peroxisome	238:247	arg1	receptor-alpha					272:285	liver peroxisome proliferator-activated receptor-alpha	232:285	liver peroxisome proliferator-activated receptor-alpha (PPAR-α) expression in obese-diabetic rats	232:328	In this study, the effect of mushroom extracellular polysaccharides on fatty acid composition and liver peroxisome proliferator-activated receptor-alpha (PPAR-α) expression in obese-diabetic rats was investigated, and distinguished the association among anti-obesity, hypoglycemic and hypolipidemic properties.
25246061	1	39	theme	peroxisome	238:247	arg1	PPAR-α					288:293	PPAR-α	288:293	PPAR-α	288:293	In this study, the effect of mushroom extracellular polysaccharides on fatty acid composition and liver peroxisome proliferator-activated receptor-alpha (PPAR-α) expression in obese-diabetic rats was investigated, and distinguished the association among anti-obesity, hypoglycemic and hypolipidemic properties.
25246061	1	40	theme	receptor-alpha	272:285	arg1	expression					296:305	liver peroxisome proliferator-activated receptor-alpha (PPAR-α) expression	232:305	liver peroxisome proliferator-activated receptor-alpha (PPAR-α) expression in obese-diabetic rats	232:328	In this study, the effect of mushroom extracellular polysaccharides on fatty acid composition and liver peroxisome proliferator-activated receptor-alpha (PPAR-α) expression in obese-diabetic rats was investigated, and distinguished the association among anti-obesity, hypoglycemic and hypolipidemic properties.
25246061	7	41	theme	low-density	1284:1294	arg1	LDL					1309:1311	LDL	1309:1311	LDL	1309:1311	Furthermore, elevated serum total cholesterol, triglycerides and low-density lipoprotein (LDL) concentrations were controlled, and parallel restoration of decreased high-density lipoprotein (HDL) levels were found with polysaccharides supplementation.
25246061	7	41	theme	low-density	1284:1294	arg1	lipoprotein					1296:1306	low-density lipoprotein	1284:1306	low-density lipoprotein (LDL)	1284:1312	Furthermore, elevated serum total cholesterol, triglycerides and low-density lipoprotein (LDL) concentrations were controlled, and parallel restoration of decreased high-density lipoprotein (HDL) levels were found with polysaccharides supplementation.
25246061	7	42	theme	elevated	1232:1239	arg1	concentrations					1314:1327	elevated serum total cholesterol, triglycerides and low-density lipoprotein (LDL) concentrations	1232:1327	elevated serum total cholesterol, triglycerides and low-density lipoprotein (LDL) concentrations	1232:1327	Furthermore, elevated serum total cholesterol, triglycerides and low-density lipoprotein (LDL) concentrations were controlled, and parallel restoration of decreased high-density lipoprotein (HDL) levels were found with polysaccharides supplementation.
25246061	7	43	theme	triglycerides	1266:1278	arg1	concentrations					1314:1327	elevated serum total cholesterol, triglycerides and low-density lipoprotein (LDL) concentrations	1232:1327	elevated serum total cholesterol, triglycerides and low-density lipoprotein (LDL) concentrations	1232:1327	Furthermore, elevated serum total cholesterol, triglycerides and low-density lipoprotein (LDL) concentrations were controlled, and parallel restoration of decreased high-density lipoprotein (HDL) levels were found with polysaccharides supplementation.
25246061	4	44	theme	=	893:893	arg1	n					891:891	n = 10/group	891:902	n = 10/group	891:902	Simultaneously to the diet, polysaccharides were orally administered to OD groups (20 mg/kg body weight/8-week), and categorized into OD+HP, OD+MP and OD+LP groups (n = 10/group), respectively.
25246061	4	44	theme	=	893:893	arg1	groups					883:888	OD+HP, OD+MP and OD+LP groups	860:888	OD+HP, OD+MP and OD+LP groups (n = 10/group)	860:903	Simultaneously to the diet, polysaccharides were orally administered to OD groups (20 mg/kg body weight/8-week), and categorized into OD+HP, OD+MP and OD+LP groups (n = 10/group), respectively.
25246061	1	45	theme	extracellular	172:184	arg1	polysaccharides					186:200	mushroom extracellular polysaccharides	163:200	mushroom extracellular polysaccharides	163:200	In this study, the effect of mushroom extracellular polysaccharides on fatty acid composition and liver peroxisome proliferator-activated receptor-alpha (PPAR-α) expression in obese-diabetic rats was investigated, and distinguished the association among anti-obesity, hypoglycemic and hypolipidemic properties.
25246061	4	46	theme	OD+MP	867:871	arg1	n					891:891	n = 10/group	891:902	n = 10/group	891:902	Simultaneously to the diet, polysaccharides were orally administered to OD groups (20 mg/kg body weight/8-week), and categorized into OD+HP, OD+MP and OD+LP groups (n = 10/group), respectively.
25246061	4	46	theme	OD+MP	867:871	arg1	groups					883:888	OD+HP, OD+MP and OD+LP groups	860:888	OD+HP, OD+MP and OD+LP groups (n = 10/group)	860:903	Simultaneously to the diet, polysaccharides were orally administered to OD groups (20 mg/kg body weight/8-week), and categorized into OD+HP, OD+MP and OD+LP groups (n = 10/group), respectively.
25246061	6	47	dep	restored	1167:1174	arg1	<					1179:1179	P < 0.01	1177:1184	P < 0.01	1177:1184	Increased fatty acid component n-6/n-3 ratio in liver and plasma of obese-diabetic rats was attenuated, while, reduced MUFA/ PUFA and MUFA/SFA ratios were restored (P < 0.01) with polysaccharides treatment.
25246061	7	48	theme	serum	1241:1245	arg1	cholesterol					1253:1263	serum total cholesterol	1241:1263	serum total cholesterol	1241:1263	Furthermore, elevated serum total cholesterol, triglycerides and low-density lipoprotein (LDL) concentrations were controlled, and parallel restoration of decreased high-density lipoprotein (HDL) levels were found with polysaccharides supplementation.
25246061	3	49	theme	Obese-	630:635	arg1	OD					647:648	OD	647:648	OD	647:648	Obese- diabetes (OD) was induced by chronic high-fat diet plus streptozotocin (STZ) injections.
25246061	3	49	theme	Obese-	630:635	arg1	diabetes					637:644	Obese- diabetes	630:644	Obese- diabetes (OD)	630:649	Obese- diabetes (OD) was induced by chronic high-fat diet plus streptozotocin (STZ) injections.
25246061	6	50	theme	Increased	1012:1020	arg1	ratio					1051:1055	Increased fatty acid component n-6/n-3 ratio	1012:1055	Increased fatty acid component n-6/n-3 ratio in liver and plasma of obese-diabetic rats	1012:1098	Increased fatty acid component n-6/n-3 ratio in liver and plasma of obese-diabetic rats was attenuated, while, reduced MUFA/ PUFA and MUFA/SFA ratios were restored (P < 0.01) with polysaccharides treatment.
25246061	4	51	theme	OD+LP	877:881	arg1	n					891:891	n = 10/group	891:902	n = 10/group	891:902	Simultaneously to the diet, polysaccharides were orally administered to OD groups (20 mg/kg body weight/8-week), and categorized into OD+HP, OD+MP and OD+LP groups (n = 10/group), respectively.
25246061	4	51	theme	OD+LP	877:881	arg1	groups					883:888	OD+HP, OD+MP and OD+LP groups	860:888	OD+HP, OD+MP and OD+LP groups (n = 10/group)	860:903	Simultaneously to the diet, polysaccharides were orally administered to OD groups (20 mg/kg body weight/8-week), and categorized into OD+HP, OD+MP and OD+LP groups (n = 10/group), respectively.
25246061	6	52	theme	P	1177:1177	arg1	<					1179:1179	P < 0.01	1177:1184	P < 0.01	1177:1184	Increased fatty acid component n-6/n-3 ratio in liver and plasma of obese-diabetic rats was attenuated, while, reduced MUFA/ PUFA and MUFA/SFA ratios were restored (P < 0.01) with polysaccharides treatment.
25246061	1	53	from	expression	296:305	arg1	rats					325:328	obese-diabetic rats	310:328	obese-diabetic rats	310:328	In this study, the effect of mushroom extracellular polysaccharides on fatty acid composition and liver peroxisome proliferator-activated receptor-alpha (PPAR-α) expression in obese-diabetic rats was investigated, and distinguished the association among anti-obesity, hypoglycemic and hypolipidemic properties.
25246061	1	54	theme	acid	211:214	arg1	composition					216:226	fatty acid composition	205:226	fatty acid composition	205:226	In this study, the effect of mushroom extracellular polysaccharides on fatty acid composition and liver peroxisome proliferator-activated receptor-alpha (PPAR-α) expression in obese-diabetic rats was investigated, and distinguished the association among anti-obesity, hypoglycemic and hypolipidemic properties.
25246061	5	55	theme	High-fat	920:927	arg1	diet					929:932	High-fat diet	920:932	High-fat diet	920:932	High-fat diet plus STZ-induced hyperglycemia was prominently attenuated by polysaccharides.
25246061	8	56	theme	protein	1570:1576	arg1	levels					1578:1583	protein levels	1570:1583	protein levels (P < 0.01)	1570:1594	This hypolipidemic property might be associated with up-regulated liver PPAR-α mRNA expression and protein levels (P < 0.01).
25246061	8	56	theme	protein	1570:1576	arg1	<					1588:1588	P < 0.01	1586:1593	P < 0.01	1586:1593	This hypolipidemic property might be associated with up-regulated liver PPAR-α mRNA expression and protein levels (P < 0.01).
25246061	8	57	theme	liver	1537:1541	arg1	expression					1555:1564	up-regulated liver PPAR-α mRNA expression	1524:1564	up-regulated liver PPAR-α mRNA expression	1524:1564	This hypolipidemic property might be associated with up-regulated liver PPAR-α mRNA expression and protein levels (P < 0.01).
25246061	3	58	theme	chronic	666:672	arg1	diet					683:686	chronic high-fat diet	666:686	chronic high-fat diet	666:686	Obese- diabetes (OD) was induced by chronic high-fat diet plus streptozotocin (STZ) injections.
25246061	1	59	from	composition	216:226	arg1	rats					325:328	obese-diabetic rats	310:328	obese-diabetic rats	310:328	In this study, the effect of mushroom extracellular polysaccharides on fatty acid composition and liver peroxisome proliferator-activated receptor-alpha (PPAR-α) expression in obese-diabetic rats was investigated, and distinguished the association among anti-obesity, hypoglycemic and hypolipidemic properties.
25246061	8	60	theme	mRNA	1550:1553	arg1	expression					1555:1564	up-regulated liver PPAR-α mRNA expression	1524:1564	up-regulated liver PPAR-α mRNA expression	1524:1564	This hypolipidemic property might be associated with up-regulated liver PPAR-α mRNA expression and protein levels (P < 0.01).
25246061	7	61	theme	lipoprotein	1296:1306	arg1	concentrations					1314:1327	elevated serum total cholesterol, triglycerides and low-density lipoprotein (LDL) concentrations	1232:1327	elevated serum total cholesterol, triglycerides and low-density lipoprotein (LDL) concentrations	1232:1327	Furthermore, elevated serum total cholesterol, triglycerides and low-density lipoprotein (LDL) concentrations were controlled, and parallel restoration of decreased high-density lipoprotein (HDL) levels were found with polysaccharides supplementation.
25246061	7	62	theme	total	1247:1251	arg1	cholesterol					1253:1263	serum total cholesterol	1241:1263	serum total cholesterol	1241:1263	Furthermore, elevated serum total cholesterol, triglycerides and low-density lipoprotein (LDL) concentrations were controlled, and parallel restoration of decreased high-density lipoprotein (HDL) levels were found with polysaccharides supplementation.
25246061	7	63	theme	high-density	1384:1395	arg1	lipoprotein					1397:1407	high-density lipoprotein	1384:1407	decreased high-density lipoprotein (HDL) levels	1374:1420	Furthermore, elevated serum total cholesterol, triglycerides and low-density lipoprotein (LDL) concentrations were controlled, and parallel restoration of decreased high-density lipoprotein (HDL) levels were found with polysaccharides supplementation.
25246061	7	63	theme	high-density	1384:1395	arg1	HDL					1410:1412	HDL	1410:1412	HDL	1410:1412	Furthermore, elevated serum total cholesterol, triglycerides and low-density lipoprotein (LDL) concentrations were controlled, and parallel restoration of decreased high-density lipoprotein (HDL) levels were found with polysaccharides supplementation.
25246061	0	64	theme	complications	96:108	arg1	progression					72:82	the progression	68:82	the progression of diabetes complications in obese-diabetic rats	68:131	Effect of Pleurotus tuber-regium polysaccharides supplementation on the progression of diabetes complications in obese-diabetic rats.
25246061	6	65	theme	n-6/n-3	1043:1049	arg1	ratio					1051:1055	Increased fatty acid component n-6/n-3 ratio	1012:1055	Increased fatty acid component n-6/n-3 ratio in liver and plasma of obese-diabetic rats	1012:1098	Increased fatty acid component n-6/n-3 ratio in liver and plasma of obese-diabetic rats was attenuated, while, reduced MUFA/ PUFA and MUFA/SFA ratios were restored (P < 0.01) with polysaccharides treatment.
25246061	6	66	from	ratio	1051:1055	arg1	plasma					1070:1075	plasma	1070:1075	plasma	1070:1075	Increased fatty acid component n-6/n-3 ratio in liver and plasma of obese-diabetic rats was attenuated, while, reduced MUFA/ PUFA and MUFA/SFA ratios were restored (P < 0.01) with polysaccharides treatment.
25246061	6	66	from	ratio	1051:1055	arg1	liver					1060:1064	liver	1060:1064	liver	1060:1064	Increased fatty acid component n-6/n-3 ratio in liver and plasma of obese-diabetic rats was attenuated, while, reduced MUFA/ PUFA and MUFA/SFA ratios were restored (P < 0.01) with polysaccharides treatment.
25246061	0	67	theme	obese-diabetic	113:126	arg1	rats					128:131	obese-diabetic rats	113:131	obese-diabetic rats	113:131	Effect of Pleurotus tuber-regium polysaccharides supplementation on the progression of diabetes complications in obese-diabetic rats.
25246061	2	68	dep	HP	560:561	arg1	high-percentage					564:578	high-percentage	564:578	high-percentage	564:578	Extracellular polysaccharides from three different strains of Pleurotus tuber-regium were extracted and labeled as HP (high-percentage), MP (medium-percentage) and LP (low-percentage).
25246061	6	69	theme	acid	1028:1031	arg1	ratio					1051:1055	Increased fatty acid component n-6/n-3 ratio	1012:1055	Increased fatty acid component n-6/n-3 ratio in liver and plasma of obese-diabetic rats	1012:1098	Increased fatty acid component n-6/n-3 ratio in liver and plasma of obese-diabetic rats was attenuated, while, reduced MUFA/ PUFA and MUFA/SFA ratios were restored (P < 0.01) with polysaccharides treatment.
25246061	8	70	theme	P	1586:1586	arg1	levels					1578:1583	protein levels	1570:1583	protein levels (P < 0.01)	1570:1594	This hypolipidemic property might be associated with up-regulated liver PPAR-α mRNA expression and protein levels (P < 0.01).
25246061	8	70	theme	P	1586:1586	arg1	<					1588:1588	P < 0.01	1586:1593	P < 0.01	1586:1593	This hypolipidemic property might be associated with up-regulated liver PPAR-α mRNA expression and protein levels (P < 0.01).
25246061	1	71	from	effect	153:158	arg1	composition					216:226	fatty acid composition	205:226	fatty acid composition	205:226	In this study, the effect of mushroom extracellular polysaccharides on fatty acid composition and liver peroxisome proliferator-activated receptor-alpha (PPAR-α) expression in obese-diabetic rats was investigated, and distinguished the association among anti-obesity, hypoglycemic and hypolipidemic properties.
25246061	1	71	from	effect	153:158	arg1	expression					296:305	liver peroxisome proliferator-activated receptor-alpha (PPAR-α) expression	232:305	liver peroxisome proliferator-activated receptor-alpha (PPAR-α) expression in obese-diabetic rats	232:328	In this study, the effect of mushroom extracellular polysaccharides on fatty acid composition and liver peroxisome proliferator-activated receptor-alpha (PPAR-α) expression in obese-diabetic rats was investigated, and distinguished the association among anti-obesity, hypoglycemic and hypolipidemic properties.
25246061	4	72	theme	mg/kg	812:816	arg1	weight/8-week					823:835	20 mg/kg body weight/8-week	809:835	20 mg/kg body weight/8-week	809:835	Simultaneously to the diet, polysaccharides were orally administered to OD groups (20 mg/kg body weight/8-week), and categorized into OD+HP, OD+MP and OD+LP groups (n = 10/group), respectively.
25246061	4	72	theme	mg/kg	812:816	arg1	groups					801:806	OD groups	798:806	OD groups (20 mg/kg body weight/8-week)	798:836	Simultaneously to the diet, polysaccharides were orally administered to OD groups (20 mg/kg body weight/8-week), and categorized into OD+HP, OD+MP and OD+LP groups (n = 10/group), respectively.
25246061	0	73	theme	tuber-regium	20:31	arg1	supplementation					49:63	Pleurotus tuber-regium polysaccharides supplementation	10:63	Pleurotus tuber-regium polysaccharides supplementation	10:63	Effect of Pleurotus tuber-regium polysaccharides supplementation on the progression of diabetes complications in obese-diabetic rats.
25246061	9	74	theme	stable	1627:1632	arg1	components					1645:1654	stable fatty acid components	1627:1654	stable fatty acid components	1627:1654	These findings concluded that stable fatty acid components and activated PPAR-α by polysaccharides may contribute to its hypoglycemic and hypolipidemic properties.
25246061	6	75	theme	rats	1095:1098	arg1	plasma					1070:1075	plasma	1070:1075	plasma	1070:1075	Increased fatty acid component n-6/n-3 ratio in liver and plasma of obese-diabetic rats was attenuated, while, reduced MUFA/ PUFA and MUFA/SFA ratios were restored (P < 0.01) with polysaccharides treatment.
25246061	6	75	theme	rats	1095:1098	arg1	liver					1060:1064	liver	1060:1064	liver	1060:1064	Increased fatty acid component n-6/n-3 ratio in liver and plasma of obese-diabetic rats was attenuated, while, reduced MUFA/ PUFA and MUFA/SFA ratios were restored (P < 0.01) with polysaccharides treatment.
25246061	2	76	theme	Pleurotus	507:515	arg1	tuber-regium					517:528	Pleurotus tuber-regium	507:528	Pleurotus tuber-regium	507:528	Extracellular polysaccharides from three different strains of Pleurotus tuber-regium were extracted and labeled as HP (high-percentage), MP (medium-percentage) and LP (low-percentage).
25246061	3	77	theme	streptozotocin	693:706	arg1	injections					714:723	chronic high-fat diet plus streptozotocin (STZ) injections	666:723	chronic high-fat diet plus streptozotocin (STZ) injections	666:723	Obese- diabetes (OD) was induced by chronic high-fat diet plus streptozotocin (STZ) injections.
25246061	0	78	theme	supplementation	49:63	arg1	Effect					0:5	Effect	0:5	Effect of Pleurotus tuber-regium polysaccharides supplementation on the progression of diabetes complications in obese-diabetic rats.	0:132	Effect of Pleurotus tuber-regium polysaccharides supplementation on the progression of diabetes complications in obese-diabetic rats.
25246061	9	79	theme	acid	1640:1643	arg1	components					1645:1654	stable fatty acid components	1627:1654	stable fatty acid components	1627:1654	These findings concluded that stable fatty acid components and activated PPAR-α by polysaccharides may contribute to its hypoglycemic and hypolipidemic properties.
25246061	3	80	theme	diet	683:686	arg1	injections					714:723	chronic high-fat diet plus streptozotocin (STZ) injections	666:723	chronic high-fat diet plus streptozotocin (STZ) injections	666:723	Obese- diabetes (OD) was induced by chronic high-fat diet plus streptozotocin (STZ) injections.
25246061	4	81	theme	OD+HP	860:864	arg1	n					891:891	n = 10/group	891:902	n = 10/group	891:902	Simultaneously to the diet, polysaccharides were orally administered to OD groups (20 mg/kg body weight/8-week), and categorized into OD+HP, OD+MP and OD+LP groups (n = 10/group), respectively.
25246061	4	81	theme	OD+HP	860:864	arg1	groups					883:888	OD+HP, OD+MP and OD+LP groups	860:888	OD+HP, OD+MP and OD+LP groups (n = 10/group)	860:903	Simultaneously to the diet, polysaccharides were orally administered to OD groups (20 mg/kg body weight/8-week), and categorized into OD+HP, OD+MP and OD+LP groups (n = 10/group), respectively.
25246061	6	82	theme	MUFA/SFA	1146:1153	arg1	ratios					1155:1160	MUFA/SFA ratios	1146:1160	MUFA/SFA ratios	1146:1160	Increased fatty acid component n-6/n-3 ratio in liver and plasma of obese-diabetic rats was attenuated, while, reduced MUFA/ PUFA and MUFA/SFA ratios were restored (P < 0.01) with polysaccharides treatment.
25246061	6	83	theme	MUFA/	1131:1135	arg1	PUFA					1137:1140	reduced MUFA/ PUFA	1123:1140	reduced MUFA/ PUFA	1123:1140	Increased fatty acid component n-6/n-3 ratio in liver and plasma of obese-diabetic rats was attenuated, while, reduced MUFA/ PUFA and MUFA/SFA ratios were restored (P < 0.01) with polysaccharides treatment.
25246061	1	84	theme	proliferator-activated	249:270	arg1	receptor-alpha					272:285	liver peroxisome proliferator-activated receptor-alpha	232:285	liver peroxisome proliferator-activated receptor-alpha (PPAR-α) expression in obese-diabetic rats	232:328	In this study, the effect of mushroom extracellular polysaccharides on fatty acid composition and liver peroxisome proliferator-activated receptor-alpha (PPAR-α) expression in obese-diabetic rats was investigated, and distinguished the association among anti-obesity, hypoglycemic and hypolipidemic properties.
25246061	1	84	theme	proliferator-activated	249:270	arg1	PPAR-α					288:293	PPAR-α	288:293	PPAR-α	288:293	In this study, the effect of mushroom extracellular polysaccharides on fatty acid composition and liver peroxisome proliferator-activated receptor-alpha (PPAR-α) expression in obese-diabetic rats was investigated, and distinguished the association among anti-obesity, hypoglycemic and hypolipidemic properties.
25246061	8	85	theme	hypolipidemic	1476:1488	arg1	property					1490:1497	This hypolipidemic property	1471:1497	This hypolipidemic property	1471:1497	This hypolipidemic property might be associated with up-regulated liver PPAR-α mRNA expression and protein levels (P < 0.01).
28224913	6	0	theme	acid	1003:1006	arg1	residues					1008:1015	the uronic acid residues	992:1015	the uronic acid residues	992:1015	Nuclear magnetic resonance spectroscopy provides complementary top-down information on the chirality of the uronic acid residues and glucosamine substitution.
28224913	1	1	attach	derived	146:152	arg2	Heparin					82:88	Heparin	82:88	Heparin	82:88	Heparin is a structurally complex, polysaccharide anticoagulant derived from livestock, primarily porcine intestinal tissues.
28224913	1	1	attach	derived	146:152	arg2	anticoagulant					132:144	a structurally complex, polysaccharide anticoagulant	93:144	a structurally complex, polysaccharide anticoagulant derived from livestock, primarily porcine intestinal tissues	93:205	Heparin is a structurally complex, polysaccharide anticoagulant derived from livestock, primarily porcine intestinal tissues.
28224913	1	1	attach	derived	146:152	arg1	livestock					159:167	livestock	159:167	livestock	159:167	Heparin is a structurally complex, polysaccharide anticoagulant derived from livestock, primarily porcine intestinal tissues.
28224913	7	2	theme	daughter	1200:1207	arg1	correlation					1209:1219	parent and daughter correlation	1189:1219	parent and daughter correlation in oligosaccharide composition	1189:1250	Principal component analysis (PCA) was applied to the normalized abundance of oligosaccharides, calculated in the bottom-up analysis, to show parent and daughter correlation in oligosaccharide composition.
28224913	2	3	theme	partial	279:285	arg1	depolymerization					287:302	the controlled partial depolymerization	264:302	the controlled partial depolymerization of heparin	264:313	Low molecular weight (LMW) heparins are derived through the controlled partial depolymerization of heparin.
28224913	11	4	theme	excellent	1779:1787	arg1	consistency					1800:1810	Lovenox®, excellent lot-to-lot consistency	1769:1810	consistency	1800:1810	The results, show similarities between LMW heparins from two different manufacturers with Lovenox®, excellent lot-to-lot consistency of products from each manufacturer, and detects a correlation between each parent heparin and daughter LMW heparin.
28224913	7	5	theme	parent	1189:1194	arg1	correlation					1209:1219	parent and daughter correlation	1189:1219	parent and daughter correlation in oligosaccharide composition	1189:1250	Principal component analysis (PCA) was applied to the normalized abundance of oligosaccharides, calculated in the bottom-up analysis, to show parent and daughter correlation in oligosaccharide composition.
28224913	5	6	theme	bottom-up	868:876	arg1	analyses					878:885	bottom-up analyses	868:885	bottom-up analyses	868:885	Liquid chromatography-mass spectrometry is rapid, robust, and amenable to automated processing and interpretation of both top-down and bottom-up analyses.
28224913	4	7	theme	oligosaccharide	704:718	arg1	composition					720:730	oligosaccharide composition	704:730	oligosaccharide composition	704:730	A strategy, for the comprehensive comparison of parent heparins and their LMW heparin daughters, is described that relies on the analysis of monosaccharide composition, disaccharide composition, and oligosaccharide composition.
28224913	1	8	theme	intestinal	188:197	arg1	tissues					199:205	porcine intestinal tissues	180:205	porcine intestinal tissues	180:205	Heparin is a structurally complex, polysaccharide anticoagulant derived from livestock, primarily porcine intestinal tissues.
28224913	8	9	theme	heparins	1297:1304	arg1	pairs					1281:1285	six pairs	1277:1285	six pairs of parent heparins	1277:1304	Using these approaches, six pairs of parent heparins and their daughter generic enoxaparins from two different manufacturers were comprehensively analyzed.
28224913	8	9	theme	heparins	1297:1304	arg1	enoxaparins					1333:1343	their daughter generic enoxaparins	1310:1343	their daughter generic enoxaparins from two different manufacturers	1310:1376	Using these approaches, six pairs of parent heparins and their daughter generic enoxaparins from two different manufacturers were comprehensively analyzed.
28224913	4	10	theme	composition	661:671	arg1	analysis					634:641	the analysis	630:641	the analysis of monosaccharide composition, disaccharide composition, and oligosaccharide composition	630:730	A strategy, for the comprehensive comparison of parent heparins and their LMW heparin daughters, is described that relies on the analysis of monosaccharide composition, disaccharide composition, and oligosaccharide composition.
28224913	4	11	theme	disaccharide	674:685	arg1	composition					687:697	disaccharide composition	674:697	disaccharide composition	674:697	A strategy, for the comprehensive comparison of parent heparins and their LMW heparin daughters, is described that relies on the analysis of monosaccharide composition, disaccharide composition, and oligosaccharide composition.
28224913	5	12	theme	analyses	878:885	arg1	interpretation					832:845	interpretation	832:845	interpretation	832:845	Liquid chromatography-mass spectrometry is rapid, robust, and amenable to automated processing and interpretation of both top-down and bottom-up analyses.
28224913	5	12	theme	analyses	878:885	arg1	processing					817:826	processing	817:826	processing	817:826	Liquid chromatography-mass spectrometry is rapid, robust, and amenable to automated processing and interpretation of both top-down and bottom-up analyses.
28224913	7	13	theme	normalized	1101:1110	arg1	abundance					1112:1120	the normalized abundance	1097:1120	the normalized abundance	1097:1120	Principal component analysis (PCA) was applied to the normalized abundance of oligosaccharides, calculated in the bottom-up analysis, to show parent and daughter correlation in oligosaccharide composition.
28224913	8	14	theme	generic	1325:1331	arg1	enoxaparins					1333:1343	their daughter generic enoxaparins	1310:1343	their daughter generic enoxaparins from two different manufacturers	1310:1376	Using these approaches, six pairs of parent heparins and their daughter generic enoxaparins from two different manufacturers were comprehensively analyzed.
28224913	6	15	theme	substitution	1033:1044	arg1	chirality					979:987	the chirality	975:987	the chirality of the uronic acid residues and glucosamine substitution	975:1044	Nuclear magnetic resonance spectroscopy provides complementary top-down information on the chirality of the uronic acid residues and glucosamine substitution.
28224913	11	16	dep	heparin	1894:1900	arg1	heparin					1919:1925	LMW heparin	1915:1925	LMW heparin	1915:1925	The results, show similarities between LMW heparins from two different manufacturers with Lovenox®, excellent lot-to-lot consistency of products from each manufacturer, and detects a correlation between each parent heparin and daughter LMW heparin.
28224913	2	17	theme	weight	222:227	arg1	heparins					235:242	Low molecular weight (LMW) heparins	208:242	Low molecular weight (LMW) heparins	208:242	Low molecular weight (LMW) heparins are derived through the controlled partial depolymerization of heparin.
28224913	1	18	theme	complex	108:114	arg1	Heparin					82:88	Heparin	82:88	Heparin	82:88	Heparin is a structurally complex, polysaccharide anticoagulant derived from livestock, primarily porcine intestinal tissues.
28224913	1	18	theme	complex	108:114	arg1	anticoagulant					132:144	a structurally complex, polysaccharide anticoagulant	93:144	a structurally complex, polysaccharide anticoagulant derived from livestock, primarily porcine intestinal tissues	93:205	Heparin is a structurally complex, polysaccharide anticoagulant derived from livestock, primarily porcine intestinal tissues.
28224913	7	19	theme	oligosaccharides	1125:1140	arg1	abundance					1112:1120	the normalized abundance	1097:1120	the normalized abundance	1097:1120	Principal component analysis (PCA) was applied to the normalized abundance of oligosaccharides, calculated in the bottom-up analysis, to show parent and daughter correlation in oligosaccharide composition.
28224913	5	20	theme	automated	807:815	arg1	processing					817:826	processing	817:826	processing	817:826	Liquid chromatography-mass spectrometry is rapid, robust, and amenable to automated processing and interpretation of both top-down and bottom-up analyses.
28224913	5	21	theme	chromatography-mass	740:758	arg1	spectrometry					760:771	Liquid chromatography-mass spectrometry	733:771	Liquid chromatography-mass spectrometry	733:771	Liquid chromatography-mass spectrometry is rapid, robust, and amenable to automated processing and interpretation of both top-down and bottom-up analyses.
28224913	10	22	theme	innovator	1568:1576	arg1	version					1578:1584	the innovator version	1564:1584	the innovator version of enoxaparin marketed in the US	1564:1617	Lovenox®, the innovator version of enoxaparin marketed in the US, was analyzed as a reference for the daughter LMW heparins.
28224913	10	22	theme	innovator	1568:1576	arg1	Lovenox®					1554:1561	Lovenox®	1554:1561	Lovenox®	1554:1561	Lovenox®, the innovator version of enoxaparin marketed in the US, was analyzed as a reference for the daughter LMW heparins.
28224913	7	23	theme	Principal	1047:1055	arg1	PCA					1077:1079	PCA	1077:1079	PCA	1077:1079	Principal component analysis (PCA) was applied to the normalized abundance of oligosaccharides, calculated in the bottom-up analysis, to show parent and daughter correlation in oligosaccharide composition.
28224913	7	23	theme	Principal	1047:1055	arg1	analysis					1067:1074	Principal component analysis	1047:1074	Principal component analysis (PCA)	1047:1080	Principal component analysis (PCA) was applied to the normalized abundance of oligosaccharides, calculated in the bottom-up analysis, to show parent and daughter correlation in oligosaccharide composition.
28224913	9	24	theme	used	1439:1442	arg1	heparin					1448:1454	the most widely used LMW heparin	1423:1454	the most widely used LMW heparin	1423:1454	Enoxaparin is the most widely used LMW heparin and is prepared through controlled chemical β-eliminative cleavage of porcine intestinal heparin.
28224913	9	24	theme	used	1439:1442	arg1	Enoxaparin					1409:1418	Enoxaparin	1409:1418	Enoxaparin	1409:1418	Enoxaparin is the most widely used LMW heparin and is prepared through controlled chemical β-eliminative cleavage of porcine intestinal heparin.
28224913	11	25	from	manufacturers	1750:1762	arg1	heparins					1722:1729	LMW heparins	1718:1729	LMW heparins from two different manufacturers with Lovenox®, excellent lot-to-lot consistency of products from each manufacturer	1718:1845	The results, show similarities between LMW heparins from two different manufacturers with Lovenox®, excellent lot-to-lot consistency of products from each manufacturer, and detects a correlation between each parent heparin and daughter LMW heparin.
28224913	0	26	theme	Parent	0:5	arg1	heparin					7:13	Parent heparin and daughter LMW heparin correlation analysis	0:59	heparin	7:13	Parent heparin and daughter LMW heparin correlation analysis using LC-MS and NMR.
28224913	11	27	from	consistency	1800:1810	arg1	manufacturer					1834:1845	each manufacturer	1829:1845	each manufacturer	1829:1845	The results, show similarities between LMW heparins from two different manufacturers with Lovenox®, excellent lot-to-lot consistency of products from each manufacturer, and detects a correlation between each parent heparin and daughter LMW heparin.
28224913	4	28	theme	LMW	579:581	arg1	daughters					591:599	their LMW heparin daughters	573:599	their LMW heparin daughters	573:599	A strategy, for the comprehensive comparison of parent heparins and their LMW heparin daughters, is described that relies on the analysis of monosaccharide composition, disaccharide composition, and oligosaccharide composition.
28224913	3	29	theme	analytical	435:444	arg1	methods					446:452	more sophisticated analytical methods	416:452	more sophisticated analytical methods	416:452	Increased manufacturing and regulatory concerns have provided the motivation for the development of more sophisticated analytical methods for determining both their structure and pedigree.
28224913	4	30	theme	daughters	591:599	arg1	comparison					539:548	the comprehensive comparison	521:548	the comprehensive comparison of parent heparins and their LMW heparin daughters	521:599	A strategy, for the comprehensive comparison of parent heparins and their LMW heparin daughters, is described that relies on the analysis of monosaccharide composition, disaccharide composition, and oligosaccharide composition.
28224913	3	31	dep	regulatory	344:353	arg1	concerns					355:362	concerns	355:362	concerns	355:362	Increased manufacturing and regulatory concerns have provided the motivation for the development of more sophisticated analytical methods for determining both their structure and pedigree.
28224913	0	32	theme	LMW	28:30	arg1	analysis					52:59	Parent heparin and daughter LMW heparin correlation analysis	0:59	analysis	52:59	Parent heparin and daughter LMW heparin correlation analysis using LC-MS and NMR.
28224913	6	33	theme	complementary	937:949	arg1	information					960:970	complementary top-down information	937:970	complementary top-down information on the chirality of the uronic acid residues and glucosamine substitution	937:1044	Nuclear magnetic resonance spectroscopy provides complementary top-down information on the chirality of the uronic acid residues and glucosamine substitution.
28224913	4	34	theme	parent	553:558	arg1	heparins					560:567	parent heparins	553:567	parent heparins	553:567	A strategy, for the comprehensive comparison of parent heparins and their LMW heparin daughters, is described that relies on the analysis of monosaccharide composition, disaccharide composition, and oligosaccharide composition.
28224913	11	35	theme	LMW	1718:1720	arg1	heparins					1722:1729	LMW heparins	1718:1729	LMW heparins from two different manufacturers with Lovenox®, excellent lot-to-lot consistency of products from each manufacturer	1718:1845	The results, show similarities between LMW heparins from two different manufacturers with Lovenox®, excellent lot-to-lot consistency of products from each manufacturer, and detects a correlation between each parent heparin and daughter LMW heparin.
28224913	0	36	theme	correlation	40:50	arg1	analysis					52:59	Parent heparin and daughter LMW heparin correlation analysis	0:59	analysis	52:59	Parent heparin and daughter LMW heparin correlation analysis using LC-MS and NMR.
28224913	9	37	theme	chemical	1491:1498	arg1	cleavage					1514:1521	controlled chemical β-eliminative cleavage	1480:1521	controlled chemical β-eliminative cleavage of porcine intestinal heparin	1480:1551	Enoxaparin is the most widely used LMW heparin and is prepared through controlled chemical β-eliminative cleavage of porcine intestinal heparin.
28224913	2	38	theme	Low	208:210	arg1	LMW					230:232	LMW	230:232	LMW	230:232	Low molecular weight (LMW) heparins are derived through the controlled partial depolymerization of heparin.
28224913	2	38	theme	Low	208:210	arg1	weight					222:227	Low molecular weight	208:227	Low molecular weight (LMW) heparins	208:242	Low molecular weight (LMW) heparins are derived through the controlled partial depolymerization of heparin.
28224913	6	39	theme	magnetic	896:903	arg1	resonance					905:913	Nuclear magnetic resonance	888:913	Nuclear magnetic resonance spectroscopy	888:926	Nuclear magnetic resonance spectroscopy provides complementary top-down information on the chirality of the uronic acid residues and glucosamine substitution.
28224913	11	40	theme	different	1740:1748	arg1	manufacturers					1750:1762	two different manufacturers	1736:1762	two different manufacturers with Lovenox®, excellent lot-to-lot consistency of products from each manufacturer	1736:1845	The results, show similarities between LMW heparins from two different manufacturers with Lovenox®, excellent lot-to-lot consistency of products from each manufacturer, and detects a correlation between each parent heparin and daughter LMW heparin.
28224913	9	41	theme	porcine	1526:1532	arg1	heparin					1545:1551	porcine intestinal heparin	1526:1551	porcine intestinal heparin	1526:1551	Enoxaparin is the most widely used LMW heparin and is prepared through controlled chemical β-eliminative cleavage of porcine intestinal heparin.
28224913	6	42	theme	residues	1008:1015	arg1	chirality					979:987	the chirality	975:987	the chirality of the uronic acid residues and glucosamine substitution	975:1044	Nuclear magnetic resonance spectroscopy provides complementary top-down information on the chirality of the uronic acid residues and glucosamine substitution.
28224913	10	43	theme	daughter	1656:1663	arg1	heparins					1669:1676	the daughter LMW heparins	1652:1676	the daughter LMW heparins	1652:1676	Lovenox®, the innovator version of enoxaparin marketed in the US, was analyzed as a reference for the daughter LMW heparins.
28224913	9	44	theme	heparin	1545:1551	arg1	cleavage					1514:1521	controlled chemical β-eliminative cleavage	1480:1521	controlled chemical β-eliminative cleavage of porcine intestinal heparin	1480:1551	Enoxaparin is the most widely used LMW heparin and is prepared through controlled chemical β-eliminative cleavage of porcine intestinal heparin.
28224913	6	45	theme	uronic	996:1001	arg1	residues					1008:1015	the uronic acid residues	992:1015	the uronic acid residues	992:1015	Nuclear magnetic resonance spectroscopy provides complementary top-down information on the chirality of the uronic acid residues and glucosamine substitution.
28224913	2	46	theme	controlled	268:277	arg1	depolymerization					287:302	the controlled partial depolymerization	264:302	the controlled partial depolymerization of heparin	264:313	Low molecular weight (LMW) heparins are derived through the controlled partial depolymerization of heparin.
28224913	8	47	theme	different	1354:1362	arg1	manufacturers					1364:1376	two different manufacturers	1350:1376	two different manufacturers	1350:1376	Using these approaches, six pairs of parent heparins and their daughter generic enoxaparins from two different manufacturers were comprehensively analyzed.
28224913	5	48	theme	top-down	855:862	arg1	interpretation					832:845	interpretation	832:845	interpretation	832:845	Liquid chromatography-mass spectrometry is rapid, robust, and amenable to automated processing and interpretation of both top-down and bottom-up analyses.
28224913	5	48	theme	top-down	855:862	arg1	processing					817:826	processing	817:826	processing	817:826	Liquid chromatography-mass spectrometry is rapid, robust, and amenable to automated processing and interpretation of both top-down and bottom-up analyses.
28224913	4	49	theme	composition	687:697	arg1	analysis					634:641	the analysis	630:641	the analysis of monosaccharide composition, disaccharide composition, and oligosaccharide composition	630:730	A strategy, for the comprehensive comparison of parent heparins and their LMW heparin daughters, is described that relies on the analysis of monosaccharide composition, disaccharide composition, and oligosaccharide composition.
28224913	11	50	theme	lot-to-lot	1789:1798	arg1	consistency					1800:1810	Lovenox®, excellent lot-to-lot consistency	1769:1810	consistency	1800:1810	The results, show similarities between LMW heparins from two different manufacturers with Lovenox®, excellent lot-to-lot consistency of products from each manufacturer, and detects a correlation between each parent heparin and daughter LMW heparin.
28224913	1	51	theme	porcine	180:186	arg1	tissues					199:205	porcine intestinal tissues	180:205	porcine intestinal tissues	180:205	Heparin is a structurally complex, polysaccharide anticoagulant derived from livestock, primarily porcine intestinal tissues.
28224913	6	52	from	information	960:970	arg1	chirality					979:987	the chirality	975:987	the chirality of the uronic acid residues and glucosamine substitution	975:1044	Nuclear magnetic resonance spectroscopy provides complementary top-down information on the chirality of the uronic acid residues and glucosamine substitution.
28224913	7	53	theme	bottom-up	1161:1169	arg1	analysis					1171:1178	the bottom-up analysis	1157:1178	the bottom-up analysis	1157:1178	Principal component analysis (PCA) was applied to the normalized abundance of oligosaccharides, calculated in the bottom-up analysis, to show parent and daughter correlation in oligosaccharide composition.
28224913	2	54	theme	heparin	307:313	arg1	depolymerization					287:302	the controlled partial depolymerization	264:302	the controlled partial depolymerization of heparin	264:313	Low molecular weight (LMW) heparins are derived through the controlled partial depolymerization of heparin.
28224913	8	55	theme	parent	1290:1295	arg1	heparins					1297:1304	parent heparins	1290:1304	parent heparins	1290:1304	Using these approaches, six pairs of parent heparins and their daughter generic enoxaparins from two different manufacturers were comprehensively analyzed.
28224913	4	56	theme	monosaccharide	646:659	arg1	composition					661:671	monosaccharide composition	646:671	monosaccharide composition	646:671	A strategy, for the comprehensive comparison of parent heparins and their LMW heparin daughters, is described that relies on the analysis of monosaccharide composition, disaccharide composition, and oligosaccharide composition.
28224913	8	57	theme	daughter	1316:1323	arg1	enoxaparins					1333:1343	their daughter generic enoxaparins	1310:1343	their daughter generic enoxaparins from two different manufacturers	1310:1376	Using these approaches, six pairs of parent heparins and their daughter generic enoxaparins from two different manufacturers were comprehensively analyzed.
28224913	6	58	theme	glucosamine	1021:1031	arg1	substitution					1033:1044	glucosamine substitution	1021:1044	glucosamine substitution	1021:1044	Nuclear magnetic resonance spectroscopy provides complementary top-down information on the chirality of the uronic acid residues and glucosamine substitution.
28224913	3	59	theme	Increased	316:324	arg1	manufacturing					326:338	Increased manufacturing	316:338	Increased manufacturing	316:338	Increased manufacturing and regulatory concerns have provided the motivation for the development of more sophisticated analytical methods for determining both their structure and pedigree.
28224913	5	60	theme	Liquid	733:738	arg1	spectrometry					760:771	Liquid chromatography-mass spectrometry	733:771	Liquid chromatography-mass spectrometry	733:771	Liquid chromatography-mass spectrometry is rapid, robust, and amenable to automated processing and interpretation of both top-down and bottom-up analyses.
28224913	11	61	theme	parent	1887:1892	arg1	heparin					1894:1900	each parent heparin	1882:1900	each parent heparin	1882:1900	The results, show similarities between LMW heparins from two different manufacturers with Lovenox®, excellent lot-to-lot consistency of products from each manufacturer, and detects a correlation between each parent heparin and daughter LMW heparin.
28224913	4	62	theme	comprehensive	525:537	arg1	comparison					539:548	the comprehensive comparison	521:548	the comprehensive comparison of parent heparins and their LMW heparin daughters	521:599	A strategy, for the comprehensive comparison of parent heparins and their LMW heparin daughters, is described that relies on the analysis of monosaccharide composition, disaccharide composition, and oligosaccharide composition.
28224913	4	63	theme	composition	720:730	arg1	analysis					634:641	the analysis	630:641	the analysis of monosaccharide composition, disaccharide composition, and oligosaccharide composition	630:730	A strategy, for the comprehensive comparison of parent heparins and their LMW heparin daughters, is described that relies on the analysis of monosaccharide composition, disaccharide composition, and oligosaccharide composition.
28224913	8	64	from	manufacturers	1364:1376	arg1	pairs					1281:1285	six pairs	1277:1285	six pairs of parent heparins	1277:1304	Using these approaches, six pairs of parent heparins and their daughter generic enoxaparins from two different manufacturers were comprehensively analyzed.
28224913	8	64	from	manufacturers	1364:1376	arg1	enoxaparins					1333:1343	their daughter generic enoxaparins	1310:1343	their daughter generic enoxaparins from two different manufacturers	1310:1376	Using these approaches, six pairs of parent heparins and their daughter generic enoxaparins from two different manufacturers were comprehensively analyzed.
28224913	11	65	theme	LMW	1915:1917	arg1	heparin					1919:1925	LMW heparin	1915:1925	LMW heparin	1915:1925	The results, show similarities between LMW heparins from two different manufacturers with Lovenox®, excellent lot-to-lot consistency of products from each manufacturer, and detects a correlation between each parent heparin and daughter LMW heparin.
28224913	7	66	theme	component	1057:1065	arg1	PCA					1077:1079	PCA	1077:1079	PCA	1077:1079	Principal component analysis (PCA) was applied to the normalized abundance of oligosaccharides, calculated in the bottom-up analysis, to show parent and daughter correlation in oligosaccharide composition.
28224913	7	66	theme	component	1057:1065	arg1	analysis					1067:1074	Principal component analysis	1047:1074	Principal component analysis (PCA)	1047:1080	Principal component analysis (PCA) was applied to the normalized abundance of oligosaccharides, calculated in the bottom-up analysis, to show parent and daughter correlation in oligosaccharide composition.
28224913	11	67	from	manufacturer	1834:1845	arg1	products					1815:1822	products	1815:1822	products from each manufacturer	1815:1845	The results, show similarities between LMW heparins from two different manufacturers with Lovenox®, excellent lot-to-lot consistency of products from each manufacturer, and detects a correlation between each parent heparin and daughter LMW heparin.
28224913	11	67	from	manufacturer	1834:1845	arg1	consistency					1800:1810	Lovenox®, excellent lot-to-lot consistency	1769:1810	consistency	1800:1810	The results, show similarities between LMW heparins from two different manufacturers with Lovenox®, excellent lot-to-lot consistency of products from each manufacturer, and detects a correlation between each parent heparin and daughter LMW heparin.
28224913	1	68	dep	complex	108:114	arg1	polysaccharide					117:130	polysaccharide	117:130	polysaccharide	117:130	Heparin is a structurally complex, polysaccharide anticoagulant derived from livestock, primarily porcine intestinal tissues.
28224913	9	69	theme	LMW	1444:1446	arg1	heparin					1448:1454	the most widely used LMW heparin	1423:1454	the most widely used LMW heparin	1423:1454	Enoxaparin is the most widely used LMW heparin and is prepared through controlled chemical β-eliminative cleavage of porcine intestinal heparin.
28224913	9	69	theme	LMW	1444:1446	arg1	Enoxaparin					1409:1418	Enoxaparin	1409:1418	Enoxaparin	1409:1418	Enoxaparin is the most widely used LMW heparin and is prepared through controlled chemical β-eliminative cleavage of porcine intestinal heparin.
28224913	4	70	theme	heparin	583:589	arg1	daughters					591:599	their LMW heparin daughters	573:599	their LMW heparin daughters	573:599	A strategy, for the comprehensive comparison of parent heparins and their LMW heparin daughters, is described that relies on the analysis of monosaccharide composition, disaccharide composition, and oligosaccharide composition.
28224913	10	71	theme	enoxaparin	1589:1598	arg1	version					1578:1584	the innovator version	1564:1584	the innovator version of enoxaparin marketed in the US	1564:1617	Lovenox®, the innovator version of enoxaparin marketed in the US, was analyzed as a reference for the daughter LMW heparins.
28224913	10	71	theme	enoxaparin	1589:1598	arg1	Lovenox®					1554:1561	Lovenox®	1554:1561	Lovenox®	1554:1561	Lovenox®, the innovator version of enoxaparin marketed in the US, was analyzed as a reference for the daughter LMW heparins.
28224913	1	72	dep	livestock	159:167	arg1	tissues					199:205	porcine intestinal tissues	180:205	porcine intestinal tissues	180:205	Heparin is a structurally complex, polysaccharide anticoagulant derived from livestock, primarily porcine intestinal tissues.
28224913	0	73	theme	daughter	19:26	arg1	analysis					52:59	Parent heparin and daughter LMW heparin correlation analysis	0:59	analysis	52:59	Parent heparin and daughter LMW heparin correlation analysis using LC-MS and NMR.
28224913	0	74	theme	heparin	32:38	arg1	analysis					52:59	Parent heparin and daughter LMW heparin correlation analysis	0:59	analysis	52:59	Parent heparin and daughter LMW heparin correlation analysis using LC-MS and NMR.
28224913	6	75	theme	top-down	951:958	arg1	information					960:970	complementary top-down information	937:970	complementary top-down information on the chirality of the uronic acid residues and glucosamine substitution	937:1044	Nuclear magnetic resonance spectroscopy provides complementary top-down information on the chirality of the uronic acid residues and glucosamine substitution.
28224913	3	76	theme	methods	446:452	arg1	development					401:411	the development	397:411	the development of more sophisticated analytical methods	397:452	Increased manufacturing and regulatory concerns have provided the motivation for the development of more sophisticated analytical methods for determining both their structure and pedigree.
28224913	4	77	theme	heparins	560:567	arg1	comparison					539:548	the comprehensive comparison	521:548	the comprehensive comparison of parent heparins and their LMW heparin daughters	521:599	A strategy, for the comprehensive comparison of parent heparins and their LMW heparin daughters, is described that relies on the analysis of monosaccharide composition, disaccharide composition, and oligosaccharide composition.
28224913	11	78	theme	products	1815:1822	arg1	consistency					1800:1810	Lovenox®, excellent lot-to-lot consistency	1769:1810	consistency	1800:1810	The results, show similarities between LMW heparins from two different manufacturers with Lovenox®, excellent lot-to-lot consistency of products from each manufacturer, and detects a correlation between each parent heparin and daughter LMW heparin.
28224913	7	79	theme	oligosaccharide	1224:1238	arg1	composition					1240:1250	oligosaccharide composition	1224:1250	oligosaccharide composition	1224:1250	Principal component analysis (PCA) was applied to the normalized abundance of oligosaccharides, calculated in the bottom-up analysis, to show parent and daughter correlation in oligosaccharide composition.
28224913	9	80	theme	controlled	1480:1489	arg1	cleavage					1514:1521	controlled chemical β-eliminative cleavage	1480:1521	controlled chemical β-eliminative cleavage of porcine intestinal heparin	1480:1551	Enoxaparin is the most widely used LMW heparin and is prepared through controlled chemical β-eliminative cleavage of porcine intestinal heparin.
28224913	3	81	theme	sophisticated	421:433	arg1	methods					446:452	more sophisticated analytical methods	416:452	more sophisticated analytical methods	416:452	Increased manufacturing and regulatory concerns have provided the motivation for the development of more sophisticated analytical methods for determining both their structure and pedigree.
28224913	7	82	from	correlation	1209:1219	arg1	composition					1240:1250	oligosaccharide composition	1224:1250	oligosaccharide composition	1224:1250	Principal component analysis (PCA) was applied to the normalized abundance of oligosaccharides, calculated in the bottom-up analysis, to show parent and daughter correlation in oligosaccharide composition.
28224913	2	83	theme	molecular	212:220	arg1	LMW					230:232	LMW	230:232	LMW	230:232	Low molecular weight (LMW) heparins are derived through the controlled partial depolymerization of heparin.
28224913	2	83	theme	molecular	212:220	arg1	weight					222:227	Low molecular weight	208:227	Low molecular weight (LMW) heparins	208:242	Low molecular weight (LMW) heparins are derived through the controlled partial depolymerization of heparin.
28224913	9	84	theme	β-eliminative	1500:1512	arg1	cleavage					1514:1521	controlled chemical β-eliminative cleavage	1480:1521	controlled chemical β-eliminative cleavage of porcine intestinal heparin	1480:1551	Enoxaparin is the most widely used LMW heparin and is prepared through controlled chemical β-eliminative cleavage of porcine intestinal heparin.
28224913	6	85	theme	resonance	905:913	arg1	spectroscopy					915:926	Nuclear magnetic resonance spectroscopy	888:926	Nuclear magnetic resonance spectroscopy	888:926	Nuclear magnetic resonance spectroscopy provides complementary top-down information on the chirality of the uronic acid residues and glucosamine substitution.
28224913	6	86	theme	Nuclear	888:894	arg1	resonance					905:913	Nuclear magnetic resonance	888:913	Nuclear magnetic resonance spectroscopy	888:926	Nuclear magnetic resonance spectroscopy provides complementary top-down information on the chirality of the uronic acid residues and glucosamine substitution.
28224913	10	87	theme	LMW	1665:1667	arg1	heparins					1669:1676	the daughter LMW heparins	1652:1676	the daughter LMW heparins	1652:1676	Lovenox®, the innovator version of enoxaparin marketed in the US, was analyzed as a reference for the daughter LMW heparins.
28224913	11	88	theme	Lovenox®	1769:1776	arg1	consistency					1800:1810	Lovenox®, excellent lot-to-lot consistency	1769:1810	consistency	1800:1810	The results, show similarities between LMW heparins from two different manufacturers with Lovenox®, excellent lot-to-lot consistency of products from each manufacturer, and detects a correlation between each parent heparin and daughter LMW heparin.
28224913	11	89	with	manufacturers	1750:1762	arg1	consistency					1800:1810	Lovenox®, excellent lot-to-lot consistency	1769:1810	consistency	1800:1810	The results, show similarities between LMW heparins from two different manufacturers with Lovenox®, excellent lot-to-lot consistency of products from each manufacturer, and detects a correlation between each parent heparin and daughter LMW heparin.
28224913	9	90	theme	intestinal	1534:1543	arg1	heparin					1545:1551	porcine intestinal heparin	1526:1551	porcine intestinal heparin	1526:1551	Enoxaparin is the most widely used LMW heparin and is prepared through controlled chemical β-eliminative cleavage of porcine intestinal heparin.
24669149	2	0	theme	Eye	486:488	arg1	Clinic					490:495	the Eye Clinic	482:495	the Eye Clinic of Bafoussam	482:508	MATERIALS AND METHODS CXL was performed in 15 eyes (11 patients) with advanced stages of keratoconus in the Eye Clinic of Bafoussam in the West Region of Cameroon.
24669149	4	1	theme	central	856:862	arg1	CCT					883:885	CCT	883:885	CCT	883:885	The central corneal thickness (CCT) was measured by ultrasound pachymetry before and after de-epithelialization and at least every 10 min during CXL.
24669149	4	1	theme	central	856:862	arg1	thickness					872:880	The central corneal thickness	852:880	The central corneal thickness (CCT)	852:886	The central corneal thickness (CCT) was measured by ultrasound pachymetry before and after de-epithelialization and at least every 10 min during CXL.
24669149	6	2	from	necessary	1467:1475	arg1	eyes					1488:1491	African eyes	1480:1491	African eyes with advanced keratoconus	1480:1517	CONCLUSIONS The combination of riboflavin compositions with swelling and stabilizing effects on the corneal stroma seems necessary in African eyes with advanced keratoconus.
24669149	2	3	theme	MATERIALS	378:386	arg1	CXL					400:402	MATERIALS AND METHODS CXL	378:402	MATERIALS AND METHODS CXL	378:402	MATERIALS AND METHODS CXL was performed in 15 eyes (11 patients) with advanced stages of keratoconus in the Eye Clinic of Bafoussam in the West Region of Cameroon.
24669149	4	4	theme	ultrasound	904:913	arg1	pachymetry					915:924	ultrasound pachymetry	904:924	ultrasound pachymetry	904:924	The central corneal thickness (CCT) was measured by ultrasound pachymetry before and after de-epithelialization and at least every 10 min during CXL.
24669149	6	5	theme	advanced	1498:1505	arg1	keratoconus					1507:1517	advanced keratoconus	1498:1517	advanced keratoconus	1498:1517	CONCLUSIONS The combination of riboflavin compositions with swelling and stabilizing effects on the corneal stroma seems necessary in African eyes with advanced keratoconus.
24669149	2	6	theme	Cameroon	532:539	arg1	Region					522:527	the West Region	513:527	the West Region of Cameroon	513:539	MATERIALS AND METHODS CXL was performed in 15 eyes (11 patients) with advanced stages of keratoconus in the Eye Clinic of Bafoussam in the West Region of Cameroon.
24669149	0	7	from	effect	11:16	arg1	thickness					78:86	the central corneal thickness	58:86	the central corneal thickness of African keratoconus corneas during collagen crosslinking	58:146	Modulatory effect of different riboflavin compositions on the central corneal thickness of African keratoconus corneas during collagen crosslinking.
24669149	5	8	theme	=	1182:1182	arg1	solution					1161:1168	solution 2	1161:1170	solution 2 (60 min/n = 5)	1161:1185	RESULTS THE APPLICATION OF THE RIBOFLAVIN SOLUTIONS RESULTED IN THE FOLLOWING MEAN FINAL CCT VALUES: 172 ± 15% using solution 1 (60 min/n = 5); 183 ± 8% using solution 2 (60 min/n = 5); 170% using solution 3 (60 min/n = 1); 80% using solution 4 (45 min/n = 1); 99% using solution 5 (45 min/n = 1) and 150 ± 13% using solution 6 (50 min/n = 2).
24669149	5	8	theme	=	1182:1182	arg1	min/n					1176:1180	60 min/n = 5	1173:1184	60 min/n = 5	1173:1184	RESULTS THE APPLICATION OF THE RIBOFLAVIN SOLUTIONS RESULTED IN THE FOLLOWING MEAN FINAL CCT VALUES: 172 ± 15% using solution 1 (60 min/n = 5); 183 ± 8% using solution 2 (60 min/n = 5); 170% using solution 3 (60 min/n = 1); 80% using solution 4 (45 min/n = 1); 99% using solution 5 (45 min/n = 1) and 150 ± 13% using solution 6 (50 min/n = 2).
24669149	2	9	theme	Bafoussam	500:508	arg1	Clinic					490:495	the Eye Clinic	482:495	the Eye Clinic of Bafoussam	482:508	MATERIALS AND METHODS CXL was performed in 15 eyes (11 patients) with advanced stages of keratoconus in the Eye Clinic of Bafoussam in the West Region of Cameroon.
24669149	0	10	theme	keratoconus	99:109	arg1	corneas					111:117	African keratoconus corneas	91:117	African keratoconus corneas	91:117	Modulatory effect of different riboflavin compositions on the central corneal thickness of African keratoconus corneas during collagen crosslinking.
24669149	6	11	from	eyes	1488:1491	arg1	necessary					1467:1475	necessary	1467:1475	necessary	1467:1475	CONCLUSIONS The combination of riboflavin compositions with swelling and stabilizing effects on the corneal stroma seems necessary in African eyes with advanced keratoconus.
24669149	7	12	theme	primary	1566:1572	arg1	results					1574:1580	these primary results	1560:1580	these primary results	1560:1580	Further studies are required to confirm these primary results.
24669149	5	13	theme	MEAN	1080:1083	arg1	VALUES					1095:1100	THE FOLLOWING MEAN FINAL CCT VALUES	1066:1100	THE FOLLOWING MEAN FINAL CCT VALUES: 172 ± 15% using solution 1 (60 min/n = 5); 183 ± 8% using solution 2 (60 min/n = 5); 170% using solution 3 (60 min/n = 1); 80% using solution 4 (45 min/n = 1); 99% using solution 5 (45 min/n = 1) and 150 ± 13% using solution 6 (50 min/n = 2).	1066:1344	RESULTS THE APPLICATION OF THE RIBOFLAVIN SOLUTIONS RESULTED IN THE FOLLOWING MEAN FINAL CCT VALUES: 172 ± 15% using solution 1 (60 min/n = 5); 183 ± 8% using solution 2 (60 min/n = 5); 170% using solution 3 (60 min/n = 1); 80% using solution 4 (45 min/n = 1); 99% using solution 5 (45 min/n = 1) and 150 ± 13% using solution 6 (50 min/n = 2).
24669149	0	14	theme	African	91:97	arg1	corneas					111:117	African keratoconus corneas	91:117	African keratoconus corneas	91:117	Modulatory effect of different riboflavin compositions on the central corneal thickness of African keratoconus corneas during collagen crosslinking.
24669149	3	15	theme	methylhydroxypropylcellulose	668:695	arg1	Solution					651:658	Solution 1	651:660	Solution 1 (0.5% methylhydroxypropylcellulose [MHPC])	651:703	The following six riboflavin compositions with different portions of active swelling additives were applied: Solution 1 (0.5% methylhydroxypropylcellulose [MHPC]), solution 2 (1.0% MHPC), solution 3 (1.7% MHPC), solution 4 (5% dextran), solution 5 (10% dextran) and solution 6 (no active swelling ingredient).
24669149	3	15	theme	methylhydroxypropylcellulose	668:695	arg1	[MHPC					697:701	0.5% methylhydroxypropylcellulose [MHPC	663:701	0.5% methylhydroxypropylcellulose [MHPC	663:701	The following six riboflavin compositions with different portions of active swelling additives were applied: Solution 1 (0.5% methylhydroxypropylcellulose [MHPC]), solution 2 (1.0% MHPC), solution 3 (1.7% MHPC), solution 4 (5% dextran), solution 5 (10% dextran) and solution 6 (no active swelling ingredient).
24669149	3	16	theme	dextran	795:801	arg1	solution					779:786	solution 5	779:788	solution 5 (10% dextran)	779:802	The following six riboflavin compositions with different portions of active swelling additives were applied: Solution 1 (0.5% methylhydroxypropylcellulose [MHPC]), solution 2 (1.0% MHPC), solution 3 (1.7% MHPC), solution 4 (5% dextran), solution 5 (10% dextran) and solution 6 (no active swelling ingredient).
24669149	3	16	theme	dextran	795:801	arg1	%					793:793	10% dextran	791:801	10% dextran	791:801	The following six riboflavin compositions with different portions of active swelling additives were applied: Solution 1 (0.5% methylhydroxypropylcellulose [MHPC]), solution 2 (1.0% MHPC), solution 3 (1.7% MHPC), solution 4 (5% dextran), solution 5 (10% dextran) and solution 6 (no active swelling ingredient).
24669149	1	17	theme	affected	286:293	arg1	eyes					303:306	clinically affected African eyes	275:306	clinically affected African eyes	275:306	PURPOSE A pilot investigation to transfer the established corneal collagen crosslinking (CXL) procedure in European eyes into clinically affected African eyes and to optimize the treatment by adapting the riboflavin composition.
24669149	2	18	from	stages	457:462	arg1	Clinic					490:495	the Eye Clinic	482:495	the Eye Clinic of Bafoussam	482:508	MATERIALS AND METHODS CXL was performed in 15 eyes (11 patients) with advanced stages of keratoconus in the Eye Clinic of Bafoussam in the West Region of Cameroon.
24669149	2	18	from	stages	457:462	arg1	Region					522:527	the West Region	513:527	the West Region of Cameroon	513:539	MATERIALS AND METHODS CXL was performed in 15 eyes (11 patients) with advanced stages of keratoconus in the Eye Clinic of Bafoussam in the West Region of Cameroon.
24669149	5	19	theme	FINAL	1085:1089	arg1	VALUES					1095:1100	THE FOLLOWING MEAN FINAL CCT VALUES	1066:1100	THE FOLLOWING MEAN FINAL CCT VALUES: 172 ± 15% using solution 1 (60 min/n = 5); 183 ± 8% using solution 2 (60 min/n = 5); 170% using solution 3 (60 min/n = 1); 80% using solution 4 (45 min/n = 1); 99% using solution 5 (45 min/n = 1) and 150 ± 13% using solution 6 (50 min/n = 2).	1066:1344	RESULTS THE APPLICATION OF THE RIBOFLAVIN SOLUTIONS RESULTED IN THE FOLLOWING MEAN FINAL CCT VALUES: 172 ± 15% using solution 1 (60 min/n = 5); 183 ± 8% using solution 2 (60 min/n = 5); 170% using solution 3 (60 min/n = 1); 80% using solution 4 (45 min/n = 1); 99% using solution 5 (45 min/n = 1) and 150 ± 13% using solution 6 (50 min/n = 2).
24669149	0	20	theme	corneas	111:117	arg1	thickness					78:86	the central corneal thickness	58:86	the central corneal thickness of African keratoconus corneas during collagen crosslinking	58:146	Modulatory effect of different riboflavin compositions on the central corneal thickness of African keratoconus corneas during collagen crosslinking.
24669149	6	21	dep	CONCLUSIONS	1346:1356	arg1	seems					1461:1465	seems	1461:1465	seems necessary in African eyes with advanced keratoconus	1461:1517	CONCLUSIONS The combination of riboflavin compositions with swelling and stabilizing effects on the corneal stroma seems necessary in African eyes with advanced keratoconus.
24669149	0	22	theme	Modulatory	0:9	arg1	effect					11:16	Modulatory effect	0:16	Modulatory effect of different riboflavin compositions on the central corneal thickness of African keratoconus corneas during collagen crosslinking.	0:147	Modulatory effect of different riboflavin compositions on the central corneal thickness of African keratoconus corneas during collagen crosslinking.
24669149	5	23	theme	±	1150:1150	arg1	%					1153:1153	183 ± 8%	1146:1153	183 ± 8%	1146:1153	RESULTS THE APPLICATION OF THE RIBOFLAVIN SOLUTIONS RESULTED IN THE FOLLOWING MEAN FINAL CCT VALUES: 172 ± 15% using solution 1 (60 min/n = 5); 183 ± 8% using solution 2 (60 min/n = 5); 170% using solution 3 (60 min/n = 1); 80% using solution 4 (45 min/n = 1); 99% using solution 5 (45 min/n = 1) and 150 ± 13% using solution 6 (50 min/n = 2).
24669149	5	24	dep	RESULTS	1002:1008	arg1	RESULTED					1054:1061	RESULTED	1054:1061	RESULTED IN THE FOLLOWING MEAN FINAL CCT VALUES: 172 ± 15% using solution 1 (60 min/n = 5); 183 ± 8% using solution 2 (60 min/n = 5); 170% using solution 3 (60 min/n = 1); 80% using solution 4 (45 min/n = 1); 99% using solution 5 (45 min/n = 1) and 150 ± 13% using solution 6 (50 min/n = 2).	1054:1344	RESULTS THE APPLICATION OF THE RIBOFLAVIN SOLUTIONS RESULTED IN THE FOLLOWING MEAN FINAL CCT VALUES: 172 ± 15% using solution 1 (60 min/n = 5); 183 ± 8% using solution 2 (60 min/n = 5); 170% using solution 3 (60 min/n = 1); 80% using solution 4 (45 min/n = 1); 99% using solution 5 (45 min/n = 1) and 150 ± 13% using solution 6 (50 min/n = 2).
24669149	2	25	theme	advanced	448:455	arg1	stages					457:462	advanced stages	448:462	advanced stages of keratoconus in the Eye Clinic of Bafoussam in the West Region of Cameroon	448:539	MATERIALS AND METHODS CXL was performed in 15 eyes (11 patients) with advanced stages of keratoconus in the Eye Clinic of Bafoussam in the West Region of Cameroon.
24669149	2	26	from	keratoconus	467:477	arg1	Clinic					490:495	the Eye Clinic	482:495	the Eye Clinic of Bafoussam	482:508	MATERIALS AND METHODS CXL was performed in 15 eyes (11 patients) with advanced stages of keratoconus in the Eye Clinic of Bafoussam in the West Region of Cameroon.
24669149	2	26	from	keratoconus	467:477	arg1	Region					522:527	the West Region	513:527	the West Region of Cameroon	513:539	MATERIALS AND METHODS CXL was performed in 15 eyes (11 patients) with advanced stages of keratoconus in the Eye Clinic of Bafoussam in the West Region of Cameroon.
24669149	5	27	theme	RIBOFLAVIN	1033:1042	arg1	SOLUTIONS					1044:1052	THE RIBOFLAVIN SOLUTIONS	1029:1052	THE RIBOFLAVIN SOLUTIONS	1029:1052	RESULTS THE APPLICATION OF THE RIBOFLAVIN SOLUTIONS RESULTED IN THE FOLLOWING MEAN FINAL CCT VALUES: 172 ± 15% using solution 1 (60 min/n = 5); 183 ± 8% using solution 2 (60 min/n = 5); 170% using solution 3 (60 min/n = 1); 80% using solution 4 (45 min/n = 1); 99% using solution 5 (45 min/n = 1) and 150 ± 13% using solution 6 (50 min/n = 2).
24669149	1	28	theme	established	195:205	arg1	procedure					243:251	the established corneal collagen crosslinking (CXL) procedure	191:251	the established corneal collagen crosslinking (CXL) procedure in European eyes	191:268	PURPOSE A pilot investigation to transfer the established corneal collagen crosslinking (CXL) procedure in European eyes into clinically affected African eyes and to optimize the treatment by adapting the riboflavin composition.
24669149	1	29	theme	riboflavin	354:363	arg1	composition					365:375	the riboflavin composition	350:375	the riboflavin composition	350:375	PURPOSE A pilot investigation to transfer the established corneal collagen crosslinking (CXL) procedure in European eyes into clinically affected African eyes and to optimize the treatment by adapting the riboflavin composition.
24669149	1	30	theme	African	295:301	arg1	eyes					303:306	clinically affected African eyes	275:306	clinically affected African eyes	275:306	PURPOSE A pilot investigation to transfer the established corneal collagen crosslinking (CXL) procedure in European eyes into clinically affected African eyes and to optimize the treatment by adapting the riboflavin composition.
24669149	5	31	theme	=	1220:1220	arg1	min/n					1214:1218	60 min/n = 1	1211:1222	60 min/n = 1	1211:1222	RESULTS THE APPLICATION OF THE RIBOFLAVIN SOLUTIONS RESULTED IN THE FOLLOWING MEAN FINAL CCT VALUES: 172 ± 15% using solution 1 (60 min/n = 5); 183 ± 8% using solution 2 (60 min/n = 5); 170% using solution 3 (60 min/n = 1); 80% using solution 4 (45 min/n = 1); 99% using solution 5 (45 min/n = 1) and 150 ± 13% using solution 6 (50 min/n = 2).
24669149	5	31	theme	=	1220:1220	arg1	solution					1199:1206	solution 3	1199:1208	solution 3 (60 min/n = 1)	1199:1223	RESULTS THE APPLICATION OF THE RIBOFLAVIN SOLUTIONS RESULTED IN THE FOLLOWING MEAN FINAL CCT VALUES: 172 ± 15% using solution 1 (60 min/n = 5); 183 ± 8% using solution 2 (60 min/n = 5); 170% using solution 3 (60 min/n = 1); 80% using solution 4 (45 min/n = 1); 99% using solution 5 (45 min/n = 1) and 150 ± 13% using solution 6 (50 min/n = 2).
24669149	6	32	theme	corneal	1446:1452	arg1	stroma					1454:1459	the corneal stroma	1442:1459	the corneal stroma	1442:1459	CONCLUSIONS The combination of riboflavin compositions with swelling and stabilizing effects on the corneal stroma seems necessary in African eyes with advanced keratoconus.
24669149	3	33	theme	riboflavin	560:569	arg1	compositions					571:582	The following six riboflavin compositions	542:582	The following six riboflavin compositions with different portions of active swelling additives	542:635	The following six riboflavin compositions with different portions of active swelling additives were applied: Solution 1 (0.5% methylhydroxypropylcellulose [MHPC]), solution 2 (1.0% MHPC), solution 3 (1.7% MHPC), solution 4 (5% dextran), solution 5 (10% dextran) and solution 6 (no active swelling ingredient).
24669149	1	34	theme	corneal	207:213	arg1	procedure					243:251	the established corneal collagen crosslinking (CXL) procedure	191:251	the established corneal collagen crosslinking (CXL) procedure in European eyes	191:268	PURPOSE A pilot investigation to transfer the established corneal collagen crosslinking (CXL) procedure in European eyes into clinically affected African eyes and to optimize the treatment by adapting the riboflavin composition.
24669149	0	35	theme	different	21:29	arg1	compositions					42:53	different riboflavin compositions	21:53	different riboflavin compositions	21:53	Modulatory effect of different riboflavin compositions on the central corneal thickness of African keratoconus corneas during collagen crosslinking.
24669149	5	36	theme	SOLUTIONS	1044:1052	arg1	APPLICATION					1014:1024	THE APPLICATION	1010:1024	THE APPLICATION OF THE RIBOFLAVIN SOLUTIONS	1010:1052	RESULTS THE APPLICATION OF THE RIBOFLAVIN SOLUTIONS RESULTED IN THE FOLLOWING MEAN FINAL CCT VALUES: 172 ± 15% using solution 1 (60 min/n = 5); 183 ± 8% using solution 2 (60 min/n = 5); 170% using solution 3 (60 min/n = 1); 80% using solution 4 (45 min/n = 1); 99% using solution 5 (45 min/n = 1) and 150 ± 13% using solution 6 (50 min/n = 2).
24669149	5	37	dep	VALUES	1095:1100	arg1	%					1111:1111	172 ± 15%	1103:1111	THE FOLLOWING MEAN FINAL CCT VALUES: 172 ± 15% using solution 1 (60 min/n = 5); 183 ± 8% using solution 2 (60 min/n = 5); 170% using solution 3 (60 min/n = 1); 80% using solution 4 (45 min/n = 1); 99% using solution 5 (45 min/n = 1) and 150 ± 13% using solution 6 (50 min/n = 2).	1066:1344	RESULTS THE APPLICATION OF THE RIBOFLAVIN SOLUTIONS RESULTED IN THE FOLLOWING MEAN FINAL CCT VALUES: 172 ± 15% using solution 1 (60 min/n = 5); 183 ± 8% using solution 2 (60 min/n = 5); 170% using solution 3 (60 min/n = 1); 80% using solution 4 (45 min/n = 1); 99% using solution 5 (45 min/n = 1) and 150 ± 13% using solution 6 (50 min/n = 2).
24669149	5	37	dep	VALUES	1095:1100	arg1	using					1155:1159	using	1155:1159	using solution 2 (60 min/n = 5)	1155:1185	RESULTS THE APPLICATION OF THE RIBOFLAVIN SOLUTIONS RESULTED IN THE FOLLOWING MEAN FINAL CCT VALUES: 172 ± 15% using solution 1 (60 min/n = 5); 183 ± 8% using solution 2 (60 min/n = 5); 170% using solution 3 (60 min/n = 1); 80% using solution 4 (45 min/n = 1); 99% using solution 5 (45 min/n = 1) and 150 ± 13% using solution 6 (50 min/n = 2).
24669149	5	38	theme	=	1140:1140	arg1	min/n					1134:1138	60 min/n = 5	1131:1142	60 min/n = 5	1131:1142	RESULTS THE APPLICATION OF THE RIBOFLAVIN SOLUTIONS RESULTED IN THE FOLLOWING MEAN FINAL CCT VALUES: 172 ± 15% using solution 1 (60 min/n = 5); 183 ± 8% using solution 2 (60 min/n = 5); 170% using solution 3 (60 min/n = 1); 80% using solution 4 (45 min/n = 1); 99% using solution 5 (45 min/n = 1) and 150 ± 13% using solution 6 (50 min/n = 2).
24669149	5	38	theme	=	1140:1140	arg1	solution					1119:1126	solution 1	1119:1128	solution 1 (60 min/n = 5)	1119:1143	RESULTS THE APPLICATION OF THE RIBOFLAVIN SOLUTIONS RESULTED IN THE FOLLOWING MEAN FINAL CCT VALUES: 172 ± 15% using solution 1 (60 min/n = 5); 183 ± 8% using solution 2 (60 min/n = 5); 170% using solution 3 (60 min/n = 1); 80% using solution 4 (45 min/n = 1); 99% using solution 5 (45 min/n = 1) and 150 ± 13% using solution 6 (50 min/n = 2).
24669149	1	39	theme	collagen	215:222	arg1	procedure					243:251	the established corneal collagen crosslinking (CXL) procedure	191:251	the established corneal collagen crosslinking (CXL) procedure in European eyes	191:268	PURPOSE A pilot investigation to transfer the established corneal collagen crosslinking (CXL) procedure in European eyes into clinically affected African eyes and to optimize the treatment by adapting the riboflavin composition.
24669149	0	40	theme	collagen	126:133	arg1	crosslinking					135:146	collagen crosslinking	126:146	collagen crosslinking	126:146	Modulatory effect of different riboflavin compositions on the central corneal thickness of African keratoconus corneas during collagen crosslinking.
24669149	3	41	theme	following	546:554	arg1	compositions					571:582	The following six riboflavin compositions	542:582	The following six riboflavin compositions with different portions of active swelling additives	542:635	The following six riboflavin compositions with different portions of active swelling additives were applied: Solution 1 (0.5% methylhydroxypropylcellulose [MHPC]), solution 2 (1.0% MHPC), solution 3 (1.7% MHPC), solution 4 (5% dextran), solution 5 (10% dextran) and solution 6 (no active swelling ingredient).
24669149	1	42	theme	crosslinking	224:235	arg1	procedure					243:251	the established corneal collagen crosslinking (CXL) procedure	191:251	the established corneal collagen crosslinking (CXL) procedure in European eyes	191:268	PURPOSE A pilot investigation to transfer the established corneal collagen crosslinking (CXL) procedure in European eyes into clinically affected African eyes and to optimize the treatment by adapting the riboflavin composition.
24669149	0	43	theme	compositions	42:53	arg1	effect					11:16	Modulatory effect	0:16	Modulatory effect of different riboflavin compositions on the central corneal thickness of African keratoconus corneas during collagen crosslinking.	0:147	Modulatory effect of different riboflavin compositions on the central corneal thickness of African keratoconus corneas during collagen crosslinking.
24669149	3	44	theme	%	721:721	arg1	MHPC					723:726	1.0% MHPC	718:726	1.0% MHPC	718:726	The following six riboflavin compositions with different portions of active swelling additives were applied: Solution 1 (0.5% methylhydroxypropylcellulose [MHPC]), solution 2 (1.0% MHPC), solution 3 (1.7% MHPC), solution 4 (5% dextran), solution 5 (10% dextran) and solution 6 (no active swelling ingredient).
24669149	3	44	theme	%	721:721	arg1	solution					706:713	solution 2	706:715	solution 2 (1.0% MHPC)	706:727	The following six riboflavin compositions with different portions of active swelling additives were applied: Solution 1 (0.5% methylhydroxypropylcellulose [MHPC]), solution 2 (1.0% MHPC), solution 3 (1.7% MHPC), solution 4 (5% dextran), solution 5 (10% dextran) and solution 6 (no active swelling ingredient).
24669149	6	45	from	effects	1431:1437	arg1	stroma					1454:1459	the corneal stroma	1442:1459	the corneal stroma	1442:1459	CONCLUSIONS The combination of riboflavin compositions with swelling and stabilizing effects on the corneal stroma seems necessary in African eyes with advanced keratoconus.
24669149	5	46	theme	=	1294:1294	arg1	min/n					1288:1292	45 min/n = 1	1285:1296	45 min/n = 1	1285:1296	RESULTS THE APPLICATION OF THE RIBOFLAVIN SOLUTIONS RESULTED IN THE FOLLOWING MEAN FINAL CCT VALUES: 172 ± 15% using solution 1 (60 min/n = 5); 183 ± 8% using solution 2 (60 min/n = 5); 170% using solution 3 (60 min/n = 1); 80% using solution 4 (45 min/n = 1); 99% using solution 5 (45 min/n = 1) and 150 ± 13% using solution 6 (50 min/n = 2).
24669149	5	46	theme	=	1294:1294	arg1	solution					1273:1280	solution 5	1273:1282	solution 5 (45 min/n = 1)	1273:1297	RESULTS THE APPLICATION OF THE RIBOFLAVIN SOLUTIONS RESULTED IN THE FOLLOWING MEAN FINAL CCT VALUES: 172 ± 15% using solution 1 (60 min/n = 5); 183 ± 8% using solution 2 (60 min/n = 5); 170% using solution 3 (60 min/n = 1); 80% using solution 4 (45 min/n = 1); 99% using solution 5 (45 min/n = 1) and 150 ± 13% using solution 6 (50 min/n = 2).
24669149	3	47	theme	different	589:597	arg1	portions					599:606	different portions	589:606	different portions of active swelling additives	589:635	The following six riboflavin compositions with different portions of active swelling additives were applied: Solution 1 (0.5% methylhydroxypropylcellulose [MHPC]), solution 2 (1.0% MHPC), solution 3 (1.7% MHPC), solution 4 (5% dextran), solution 5 (10% dextran) and solution 6 (no active swelling ingredient).
24669149	6	48	from	swelling	1406:1413	arg1	stroma					1454:1459	the corneal stroma	1442:1459	the corneal stroma	1442:1459	CONCLUSIONS The combination of riboflavin compositions with swelling and stabilizing effects on the corneal stroma seems necessary in African eyes with advanced keratoconus.
24669149	5	49	theme	FOLLOWING	1070:1078	arg1	VALUES					1095:1100	THE FOLLOWING MEAN FINAL CCT VALUES	1066:1100	THE FOLLOWING MEAN FINAL CCT VALUES: 172 ± 15% using solution 1 (60 min/n = 5); 183 ± 8% using solution 2 (60 min/n = 5); 170% using solution 3 (60 min/n = 1); 80% using solution 4 (45 min/n = 1); 99% using solution 5 (45 min/n = 1) and 150 ± 13% using solution 6 (50 min/n = 2).	1066:1344	RESULTS THE APPLICATION OF THE RIBOFLAVIN SOLUTIONS RESULTED IN THE FOLLOWING MEAN FINAL CCT VALUES: 172 ± 15% using solution 1 (60 min/n = 5); 183 ± 8% using solution 2 (60 min/n = 5); 170% using solution 3 (60 min/n = 1); 80% using solution 4 (45 min/n = 1); 99% using solution 5 (45 min/n = 1) and 150 ± 13% using solution 6 (50 min/n = 2).
24669149	0	50	theme	riboflavin	31:40	arg1	compositions					42:53	different riboflavin compositions	21:53	different riboflavin compositions	21:53	Modulatory effect of different riboflavin compositions on the central corneal thickness of African keratoconus corneas during collagen crosslinking.
24669149	2	51	theme	keratoconus	467:477	arg1	stages					457:462	advanced stages	448:462	advanced stages of keratoconus in the Eye Clinic of Bafoussam in the West Region of Cameroon	448:539	MATERIALS AND METHODS CXL was performed in 15 eyes (11 patients) with advanced stages of keratoconus in the Eye Clinic of Bafoussam in the West Region of Cameroon.
24669149	3	52	theme	swelling	830:837	arg1	solution					808:815	solution 6	808:817	solution 6 (no active swelling ingredient)	808:849	The following six riboflavin compositions with different portions of active swelling additives were applied: Solution 1 (0.5% methylhydroxypropylcellulose [MHPC]), solution 2 (1.0% MHPC), solution 3 (1.7% MHPC), solution 4 (5% dextran), solution 5 (10% dextran) and solution 6 (no active swelling ingredient).
24669149	3	52	theme	swelling	830:837	arg1	ingredient					839:848	no active swelling ingredient	820:848	no active swelling ingredient	820:848	The following six riboflavin compositions with different portions of active swelling additives were applied: Solution 1 (0.5% methylhydroxypropylcellulose [MHPC]), solution 2 (1.0% MHPC), solution 3 (1.7% MHPC), solution 4 (5% dextran), solution 5 (10% dextran) and solution 6 (no active swelling ingredient).
24669149	1	53	theme	CXL	238:240	arg1	procedure					243:251	the established corneal collagen crosslinking (CXL) procedure	191:251	the established corneal collagen crosslinking (CXL) procedure in European eyes	191:268	PURPOSE A pilot investigation to transfer the established corneal collagen crosslinking (CXL) procedure in European eyes into clinically affected African eyes and to optimize the treatment by adapting the riboflavin composition.
24669149	2	54	theme	West	517:520	arg1	Region					522:527	the West Region	513:527	the West Region of Cameroon	513:539	MATERIALS AND METHODS CXL was performed in 15 eyes (11 patients) with advanced stages of keratoconus in the Eye Clinic of Bafoussam in the West Region of Cameroon.
24669149	2	55	from	Clinic	490:495	arg1	stages					457:462	advanced stages	448:462	advanced stages of keratoconus in the Eye Clinic of Bafoussam in the West Region of Cameroon	448:539	MATERIALS AND METHODS CXL was performed in 15 eyes (11 patients) with advanced stages of keratoconus in the Eye Clinic of Bafoussam in the West Region of Cameroon.
24669149	5	56	theme	=	1257:1257	arg1	solution					1236:1243	solution 4	1236:1245	solution 4 (45 min/n = 1)	1236:1260	RESULTS THE APPLICATION OF THE RIBOFLAVIN SOLUTIONS RESULTED IN THE FOLLOWING MEAN FINAL CCT VALUES: 172 ± 15% using solution 1 (60 min/n = 5); 183 ± 8% using solution 2 (60 min/n = 5); 170% using solution 3 (60 min/n = 1); 80% using solution 4 (45 min/n = 1); 99% using solution 5 (45 min/n = 1) and 150 ± 13% using solution 6 (50 min/n = 2).
24669149	5	56	theme	=	1257:1257	arg1	min/n					1251:1255	45 min/n = 1	1248:1259	45 min/n = 1	1248:1259	RESULTS THE APPLICATION OF THE RIBOFLAVIN SOLUTIONS RESULTED IN THE FOLLOWING MEAN FINAL CCT VALUES: 172 ± 15% using solution 1 (60 min/n = 5); 183 ± 8% using solution 2 (60 min/n = 5); 170% using solution 3 (60 min/n = 1); 80% using solution 4 (45 min/n = 1); 99% using solution 5 (45 min/n = 1) and 150 ± 13% using solution 6 (50 min/n = 2).
24669149	6	57	theme	riboflavin	1377:1386	arg1	compositions					1388:1399	riboflavin compositions	1377:1399	riboflavin compositions	1377:1399	CONCLUSIONS The combination of riboflavin compositions with swelling and stabilizing effects on the corneal stroma seems necessary in African eyes with advanced keratoconus.
24669149	7	58	theme	Further	1520:1526	arg1	studies					1528:1534	Further studies	1520:1534	Further studies	1520:1534	Further studies are required to confirm these primary results.
24669149	6	59	with	eyes	1488:1491	arg1	keratoconus					1507:1517	advanced keratoconus	1498:1517	advanced keratoconus	1498:1517	CONCLUSIONS The combination of riboflavin compositions with swelling and stabilizing effects on the corneal stroma seems necessary in African eyes with advanced keratoconus.
24669149	3	60	with	compositions	571:582	arg1	portions					599:606	different portions	589:606	different portions of active swelling additives	589:635	The following six riboflavin compositions with different portions of active swelling additives were applied: Solution 1 (0.5% methylhydroxypropylcellulose [MHPC]), solution 2 (1.0% MHPC), solution 3 (1.7% MHPC), solution 4 (5% dextran), solution 5 (10% dextran) and solution 6 (no active swelling ingredient).
24669149	3	61	theme	dextran	769:775	arg1	solution					754:761	solution 4	754:763	solution 4 (5% dextran)	754:776	The following six riboflavin compositions with different portions of active swelling additives were applied: Solution 1 (0.5% methylhydroxypropylcellulose [MHPC]), solution 2 (1.0% MHPC), solution 3 (1.7% MHPC), solution 4 (5% dextran), solution 5 (10% dextran) and solution 6 (no active swelling ingredient).
24669149	3	61	theme	dextran	769:775	arg1	%					767:767	5% dextran	766:775	5% dextran	766:775	The following six riboflavin compositions with different portions of active swelling additives were applied: Solution 1 (0.5% methylhydroxypropylcellulose [MHPC]), solution 2 (1.0% MHPC), solution 3 (1.7% MHPC), solution 4 (5% dextran), solution 5 (10% dextran) and solution 6 (no active swelling ingredient).
24669149	3	62	theme	active	611:616	arg1	additives					627:635	active swelling additives	611:635	active swelling additives	611:635	The following six riboflavin compositions with different portions of active swelling additives were applied: Solution 1 (0.5% methylhydroxypropylcellulose [MHPC]), solution 2 (1.0% MHPC), solution 3 (1.7% MHPC), solution 4 (5% dextran), solution 5 (10% dextran) and solution 6 (no active swelling ingredient).
24669149	3	63	theme	%	666:666	arg1	Solution					651:658	Solution 1	651:660	Solution 1 (0.5% methylhydroxypropylcellulose [MHPC])	651:703	The following six riboflavin compositions with different portions of active swelling additives were applied: Solution 1 (0.5% methylhydroxypropylcellulose [MHPC]), solution 2 (1.0% MHPC), solution 3 (1.7% MHPC), solution 4 (5% dextran), solution 5 (10% dextran) and solution 6 (no active swelling ingredient).
24669149	3	63	theme	%	666:666	arg1	[MHPC					697:701	0.5% methylhydroxypropylcellulose [MHPC	663:701	0.5% methylhydroxypropylcellulose [MHPC	663:701	The following six riboflavin compositions with different portions of active swelling additives were applied: Solution 1 (0.5% methylhydroxypropylcellulose [MHPC]), solution 2 (1.0% MHPC), solution 3 (1.7% MHPC), solution 4 (5% dextran), solution 5 (10% dextran) and solution 6 (no active swelling ingredient).
24669149	3	64	theme	swelling	618:625	arg1	additives					627:635	active swelling additives	611:635	active swelling additives	611:635	The following six riboflavin compositions with different portions of active swelling additives were applied: Solution 1 (0.5% methylhydroxypropylcellulose [MHPC]), solution 2 (1.0% MHPC), solution 3 (1.7% MHPC), solution 4 (5% dextran), solution 5 (10% dextran) and solution 6 (no active swelling ingredient).
24669149	3	65	theme	active	823:828	arg1	solution					808:815	solution 6	808:817	solution 6 (no active swelling ingredient)	808:849	The following six riboflavin compositions with different portions of active swelling additives were applied: Solution 1 (0.5% methylhydroxypropylcellulose [MHPC]), solution 2 (1.0% MHPC), solution 3 (1.7% MHPC), solution 4 (5% dextran), solution 5 (10% dextran) and solution 6 (no active swelling ingredient).
24669149	3	65	theme	active	823:828	arg1	ingredient					839:848	no active swelling ingredient	820:848	no active swelling ingredient	820:848	The following six riboflavin compositions with different portions of active swelling additives were applied: Solution 1 (0.5% methylhydroxypropylcellulose [MHPC]), solution 2 (1.0% MHPC), solution 3 (1.7% MHPC), solution 4 (5% dextran), solution 5 (10% dextran) and solution 6 (no active swelling ingredient).
24669149	6	66	with	combination	1362:1372	arg1	swelling					1406:1413	swelling	1406:1413	swelling	1406:1413	CONCLUSIONS The combination of riboflavin compositions with swelling and stabilizing effects on the corneal stroma seems necessary in African eyes with advanced keratoconus.
24669149	6	66	with	combination	1362:1372	arg1	effects					1431:1437	stabilizing effects	1419:1437	stabilizing effects on the corneal stroma	1419:1459	CONCLUSIONS The combination of riboflavin compositions with swelling and stabilizing effects on the corneal stroma seems necessary in African eyes with advanced keratoconus.
24669149	4	67	theme	corneal	864:870	arg1	CCT					883:885	CCT	883:885	CCT	883:885	The central corneal thickness (CCT) was measured by ultrasound pachymetry before and after de-epithelialization and at least every 10 min during CXL.
24669149	4	67	theme	corneal	864:870	arg1	thickness					872:880	The central corneal thickness	852:880	The central corneal thickness (CCT)	852:886	The central corneal thickness (CCT) was measured by ultrasound pachymetry before and after de-epithelialization and at least every 10 min during CXL.
24669149	5	68	theme	CCT	1091:1093	arg1	VALUES					1095:1100	THE FOLLOWING MEAN FINAL CCT VALUES	1066:1100	THE FOLLOWING MEAN FINAL CCT VALUES: 172 ± 15% using solution 1 (60 min/n = 5); 183 ± 8% using solution 2 (60 min/n = 5); 170% using solution 3 (60 min/n = 1); 80% using solution 4 (45 min/n = 1); 99% using solution 5 (45 min/n = 1) and 150 ± 13% using solution 6 (50 min/n = 2).	1066:1344	RESULTS THE APPLICATION OF THE RIBOFLAVIN SOLUTIONS RESULTED IN THE FOLLOWING MEAN FINAL CCT VALUES: 172 ± 15% using solution 1 (60 min/n = 5); 183 ± 8% using solution 2 (60 min/n = 5); 170% using solution 3 (60 min/n = 1); 80% using solution 4 (45 min/n = 1); 99% using solution 5 (45 min/n = 1) and 150 ± 13% using solution 6 (50 min/n = 2).
24669149	1	69	from	procedure	243:251	arg1	eyes					265:268	European eyes	256:268	European eyes	256:268	PURPOSE A pilot investigation to transfer the established corneal collagen crosslinking (CXL) procedure in European eyes into clinically affected African eyes and to optimize the treatment by adapting the riboflavin composition.
24669149	3	70	theme	additives	627:635	arg1	portions					599:606	different portions	589:606	different portions of active swelling additives	589:635	The following six riboflavin compositions with different portions of active swelling additives were applied: Solution 1 (0.5% methylhydroxypropylcellulose [MHPC]), solution 2 (1.0% MHPC), solution 3 (1.7% MHPC), solution 4 (5% dextran), solution 5 (10% dextran) and solution 6 (no active swelling ingredient).
24669149	5	71	theme	=	1340:1340	arg1	min/n					1334:1338	50 min/n = 2	1331:1342	50 min/n = 2	1331:1342	RESULTS THE APPLICATION OF THE RIBOFLAVIN SOLUTIONS RESULTED IN THE FOLLOWING MEAN FINAL CCT VALUES: 172 ± 15% using solution 1 (60 min/n = 5); 183 ± 8% using solution 2 (60 min/n = 5); 170% using solution 3 (60 min/n = 1); 80% using solution 4 (45 min/n = 1); 99% using solution 5 (45 min/n = 1) and 150 ± 13% using solution 6 (50 min/n = 2).
24669149	5	71	theme	=	1340:1340	arg1	solution					1319:1326	solution 6	1319:1328	solution 6 (50 min/n = 2)	1319:1343	RESULTS THE APPLICATION OF THE RIBOFLAVIN SOLUTIONS RESULTED IN THE FOLLOWING MEAN FINAL CCT VALUES: 172 ± 15% using solution 1 (60 min/n = 5); 183 ± 8% using solution 2 (60 min/n = 5); 170% using solution 3 (60 min/n = 1); 80% using solution 4 (45 min/n = 1); 99% using solution 5 (45 min/n = 1) and 150 ± 13% using solution 6 (50 min/n = 2).
24669149	2	72	from	Region	522:527	arg1	stages					457:462	advanced stages	448:462	advanced stages of keratoconus in the Eye Clinic of Bafoussam in the West Region of Cameroon	448:539	MATERIALS AND METHODS CXL was performed in 15 eyes (11 patients) with advanced stages of keratoconus in the Eye Clinic of Bafoussam in the West Region of Cameroon.
24669149	0	73	theme	corneal	70:76	arg1	thickness					78:86	the central corneal thickness	58:86	the central corneal thickness of African keratoconus corneas during collagen crosslinking	58:146	Modulatory effect of different riboflavin compositions on the central corneal thickness of African keratoconus corneas during collagen crosslinking.
24669149	6	74	theme	African	1480:1486	arg1	eyes					1488:1491	African eyes	1480:1491	African eyes with advanced keratoconus	1480:1517	CONCLUSIONS The combination of riboflavin compositions with swelling and stabilizing effects on the corneal stroma seems necessary in African eyes with advanced keratoconus.
24669149	5	75	theme	±	1307:1307	arg1	%					1311:1311	150 ± 13%	1303:1311	150 ± 13% using solution 6 (50 min/n = 2)	1303:1343	RESULTS THE APPLICATION OF THE RIBOFLAVIN SOLUTIONS RESULTED IN THE FOLLOWING MEAN FINAL CCT VALUES: 172 ± 15% using solution 1 (60 min/n = 5); 183 ± 8% using solution 2 (60 min/n = 5); 170% using solution 3 (60 min/n = 1); 80% using solution 4 (45 min/n = 1); 99% using solution 5 (45 min/n = 1) and 150 ± 13% using solution 6 (50 min/n = 2).
24669149	2	76	theme	METHODS	392:398	arg1	CXL					400:402	MATERIALS AND METHODS CXL	378:402	MATERIALS AND METHODS CXL	378:402	MATERIALS AND METHODS CXL was performed in 15 eyes (11 patients) with advanced stages of keratoconus in the Eye Clinic of Bafoussam in the West Region of Cameroon.
24669149	6	77	theme	stabilizing	1419:1429	arg1	effects					1431:1437	stabilizing effects	1419:1437	stabilizing effects on the corneal stroma	1419:1459	CONCLUSIONS The combination of riboflavin compositions with swelling and stabilizing effects on the corneal stroma seems necessary in African eyes with advanced keratoconus.
24669149	0	78	theme	central	62:68	arg1	thickness					78:86	the central corneal thickness	58:86	the central corneal thickness of African keratoconus corneas during collagen crosslinking	58:146	Modulatory effect of different riboflavin compositions on the central corneal thickness of African keratoconus corneas during collagen crosslinking.
24669149	6	79	theme	compositions	1388:1399	arg1	combination					1362:1372	The combination	1358:1372	The combination of riboflavin compositions with swelling and stabilizing effects on the corneal stroma	1358:1459	CONCLUSIONS The combination of riboflavin compositions with swelling and stabilizing effects on the corneal stroma seems necessary in African eyes with advanced keratoconus.
24669149	3	80	theme	%	745:745	arg1	MHPC					747:750	1.7% MHPC	742:750	1.7% MHPC	742:750	The following six riboflavin compositions with different portions of active swelling additives were applied: Solution 1 (0.5% methylhydroxypropylcellulose [MHPC]), solution 2 (1.0% MHPC), solution 3 (1.7% MHPC), solution 4 (5% dextran), solution 5 (10% dextran) and solution 6 (no active swelling ingredient).
24669149	3	80	theme	%	745:745	arg1	solution					730:737	solution 3	730:739	solution 3 (1.7% MHPC)	730:751	The following six riboflavin compositions with different portions of active swelling additives were applied: Solution 1 (0.5% methylhydroxypropylcellulose [MHPC]), solution 2 (1.0% MHPC), solution 3 (1.7% MHPC), solution 4 (5% dextran), solution 5 (10% dextran) and solution 6 (no active swelling ingredient).
24669149	1	81	theme	pilot	159:163	arg1	investigation					165:177	A pilot investigation	157:177	A pilot investigation	157:177	PURPOSE A pilot investigation to transfer the established corneal collagen crosslinking (CXL) procedure in European eyes into clinically affected African eyes and to optimize the treatment by adapting the riboflavin composition.
24669149	5	82	theme	±	1107:1107	arg1	%					1111:1111	172 ± 15%	1103:1111	THE FOLLOWING MEAN FINAL CCT VALUES: 172 ± 15% using solution 1 (60 min/n = 5); 183 ± 8% using solution 2 (60 min/n = 5); 170% using solution 3 (60 min/n = 1); 80% using solution 4 (45 min/n = 1); 99% using solution 5 (45 min/n = 1) and 150 ± 13% using solution 6 (50 min/n = 2).	1066:1344	RESULTS THE APPLICATION OF THE RIBOFLAVIN SOLUTIONS RESULTED IN THE FOLLOWING MEAN FINAL CCT VALUES: 172 ± 15% using solution 1 (60 min/n = 5); 183 ± 8% using solution 2 (60 min/n = 5); 170% using solution 3 (60 min/n = 1); 80% using solution 4 (45 min/n = 1); 99% using solution 5 (45 min/n = 1) and 150 ± 13% using solution 6 (50 min/n = 2).
24669149	1	83	theme	European	256:263	arg1	eyes					265:268	European eyes	256:268	European eyes	256:268	PURPOSE A pilot investigation to transfer the established corneal collagen crosslinking (CXL) procedure in European eyes into clinically affected African eyes and to optimize the treatment by adapting the riboflavin composition.
24708950	0	0	theme	polyester	105:113	arg1	composites					115:124	cross-linked cellulose acetate-reinforced polyester composites	63:124	cross-linked cellulose acetate-reinforced polyester composites	63:124	Mechanical properties, biocompatibility, and biodegradation of cross-linked cellulose acetate-reinforced polyester composites.
24708950	0	1	theme	acetate-reinforced	86:103	arg1	composites					115:124	cross-linked cellulose acetate-reinforced polyester composites	63:124	cross-linked cellulose acetate-reinforced polyester composites	63:124	Mechanical properties, biocompatibility, and biodegradation of cross-linked cellulose acetate-reinforced polyester composites.
24708950	4	2	theme	PHA/CA	733:738	arg1	series					740:745	the PHA/CA series	729:745	the PHA/CA series of composites	729:759	In a time-dependent course, the FB proliferation results demonstrated higher performance from the PHA/CA series of composites than from the PHA-g-AA/t-CA composites.
24708950	1	3	theme	superior	274:281	arg1	properties					294:303	noticeably superior mechanical properties	263:303	noticeably superior mechanical properties	263:303	Composites of treated (cross-linked) cellulose acetate (t-CA) and acrylic acid-grafted poly(hydroxyalkanoate) (PHA-g-AA/t-CA) exhibited noticeably superior mechanical properties compared with PHA/CA composites due to greater compatibility between the two components.
24708950	5	4	theme	Acetobacter	925:935	arg1	pasteurianus					937:948	Acetobacter pasteurianus	925:948	Acetobacter pasteurianus (A. pasteurianus)	925:966	The water resistance of PHA-g-AA/t-CA was higher than that of PHA/CA, although the weight loss of both composites buried in Acetobacter pasteurianus (A. pasteurianus) indicated that they were both biodegradable, especially at higher levels of cellulose acetate substitution.
24708950	5	4	theme	Acetobacter	925:935	arg1	pasteurianus					954:965	A. pasteurianus	951:965	A. pasteurianus	951:965	The water resistance of PHA-g-AA/t-CA was higher than that of PHA/CA, although the weight loss of both composites buried in Acetobacter pasteurianus (A. pasteurianus) indicated that they were both biodegradable, especially at higher levels of cellulose acetate substitution.
24708950	6	5	theme	cellulose	1192:1200	arg1	content					1210:1216	cellulose acetate content	1192:1216	cellulose acetate content	1192:1216	The PHA/CA and PHA-g-AA/t-CA composites were more biodegradable than pure PHA, implying a strong connection between cellulose acetate content and biodegradability.
24708950	1	6	theme	mechanical	283:292	arg1	properties					294:303	noticeably superior mechanical properties	263:303	noticeably superior mechanical properties	263:303	Composites of treated (cross-linked) cellulose acetate (t-CA) and acrylic acid-grafted poly(hydroxyalkanoate) (PHA-g-AA/t-CA) exhibited noticeably superior mechanical properties compared with PHA/CA composites due to greater compatibility between the two components.
24708950	0	7	theme	composites	115:124	arg1	biocompatibility					23:38	biocompatibility	23:38	biocompatibility	23:38	Mechanical properties, biocompatibility, and biodegradation of cross-linked cellulose acetate-reinforced polyester composites.
24708950	0	7	theme	composites	115:124	arg1	biodegradation					45:58	biodegradation	45:58	biodegradation	45:58	Mechanical properties, biocompatibility, and biodegradation of cross-linked cellulose acetate-reinforced polyester composites.
24708950	0	7	theme	composites	115:124	arg1	properties					11:20	Mechanical properties	0:20	Mechanical properties	0:20	Mechanical properties, biocompatibility, and biodegradation of cross-linked cellulose acetate-reinforced polyester composites.
24708950	4	8	theme	composites	750:759	arg1	series					740:745	the PHA/CA series	729:745	the PHA/CA series of composites	729:759	In a time-dependent course, the FB proliferation results demonstrated higher performance from the PHA/CA series of composites than from the PHA-g-AA/t-CA composites.
24708950	6	9	theme	PHA-g-AA/t-CA	1091:1103	arg1	composites					1105:1114	The PHA/CA and PHA-g-AA/t-CA composites	1076:1114	The PHA/CA and PHA-g-AA/t-CA composites	1076:1114	The PHA/CA and PHA-g-AA/t-CA composites were more biodegradable than pure PHA, implying a strong connection between cellulose acetate content and biodegradability.
24708950	6	9	theme	PHA-g-AA/t-CA	1091:1103	arg1	biodegradable					1126:1138	biodegradable	1126:1138	biodegradable	1126:1138	The PHA/CA and PHA-g-AA/t-CA composites were more biodegradable than pure PHA, implying a strong connection between cellulose acetate content and biodegradability.
24708950	1	10	dep	treated	141:147	arg1	cross-linked					150:161	cross-linked	150:161	cross-linked	150:161	Composites of treated (cross-linked) cellulose acetate (t-CA) and acrylic acid-grafted poly(hydroxyalkanoate) (PHA-g-AA/t-CA) exhibited noticeably superior mechanical properties compared with PHA/CA composites due to greater compatibility between the two components.
24708950	2	11	theme	t-CA	421:424	arg1	homogeneous					460:470	homogeneous	460:470	homogeneous	460:470	The dispersion covering of t-CA in the PHA-g-AA matrix was highly homogeneous as a result of condensation reactions.
24708950	2	11	theme	t-CA	421:424	arg1	dispersion					398:407	The dispersion covering	394:416	The dispersion covering of t-CA in the PHA-g-AA matrix	394:447	The dispersion covering of t-CA in the PHA-g-AA matrix was highly homogeneous as a result of condensation reactions.
24708950	6	12	theme	pure	1145:1148	arg1	PHA					1150:1152	pure PHA	1145:1152	pure PHA	1145:1152	The PHA/CA and PHA-g-AA/t-CA composites were more biodegradable than pure PHA, implying a strong connection between cellulose acetate content and biodegradability.
24708950	4	13	theme	PHA-g-AA/t-CA	775:787	arg1	composites					789:798	the PHA-g-AA/t-CA composites	771:798	the PHA-g-AA/t-CA composites	771:798	In a time-dependent course, the FB proliferation results demonstrated higher performance from the PHA/CA series of composites than from the PHA-g-AA/t-CA composites.
24708950	5	14	from	levels	1034:1039	arg1	biodegradable					998:1010	biodegradable	998:1010	biodegradable	998:1010	The water resistance of PHA-g-AA/t-CA was higher than that of PHA/CA, although the weight loss of both composites buried in Acetobacter pasteurianus (A. pasteurianus) indicated that they were both biodegradable, especially at higher levels of cellulose acetate substitution.
24708950	1	15	theme	acrylic	193:199	arg1	hydroxyalkanoate					219:234	hydroxyalkanoate	219:234	hydroxyalkanoate	219:234	Composites of treated (cross-linked) cellulose acetate (t-CA) and acrylic acid-grafted poly(hydroxyalkanoate) (PHA-g-AA/t-CA) exhibited noticeably superior mechanical properties compared with PHA/CA composites due to greater compatibility between the two components.
24708950	1	15	theme	acrylic	193:199	arg1	PHA-g-AA/t-CA					238:250	PHA-g-AA/t-CA	238:250	PHA-g-AA/t-CA	238:250	Composites of treated (cross-linked) cellulose acetate (t-CA) and acrylic acid-grafted poly(hydroxyalkanoate) (PHA-g-AA/t-CA) exhibited noticeably superior mechanical properties compared with PHA/CA composites due to greater compatibility between the two components.
24708950	1	15	theme	acrylic	193:199	arg1	poly					214:217	acrylic acid-grafted poly	193:217	acrylic acid-grafted poly(hydroxyalkanoate) (PHA-g-AA/t-CA)	193:251	Composites of treated (cross-linked) cellulose acetate (t-CA) and acrylic acid-grafted poly(hydroxyalkanoate) (PHA-g-AA/t-CA) exhibited noticeably superior mechanical properties compared with PHA/CA composites due to greater compatibility between the two components.
24708950	0	16	theme	Mechanical	0:9	arg1	properties					11:20	Mechanical properties	0:20	Mechanical properties	0:20	Mechanical properties, biocompatibility, and biodegradation of cross-linked cellulose acetate-reinforced polyester composites.
24708950	1	17	theme	acid-grafted	201:212	arg1	hydroxyalkanoate					219:234	hydroxyalkanoate	219:234	hydroxyalkanoate	219:234	Composites of treated (cross-linked) cellulose acetate (t-CA) and acrylic acid-grafted poly(hydroxyalkanoate) (PHA-g-AA/t-CA) exhibited noticeably superior mechanical properties compared with PHA/CA composites due to greater compatibility between the two components.
24708950	1	17	theme	acid-grafted	201:212	arg1	PHA-g-AA/t-CA					238:250	PHA-g-AA/t-CA	238:250	PHA-g-AA/t-CA	238:250	Composites of treated (cross-linked) cellulose acetate (t-CA) and acrylic acid-grafted poly(hydroxyalkanoate) (PHA-g-AA/t-CA) exhibited noticeably superior mechanical properties compared with PHA/CA composites due to greater compatibility between the two components.
24708950	1	17	theme	acid-grafted	201:212	arg1	poly					214:217	acrylic acid-grafted poly	193:217	acrylic acid-grafted poly(hydroxyalkanoate) (PHA-g-AA/t-CA)	193:251	Composites of treated (cross-linked) cellulose acetate (t-CA) and acrylic acid-grafted poly(hydroxyalkanoate) (PHA-g-AA/t-CA) exhibited noticeably superior mechanical properties compared with PHA/CA composites due to greater compatibility between the two components.
24708950	2	18	from	dispersion	398:407	arg1	matrix					442:447	the PHA-g-AA matrix	429:447	the PHA-g-AA matrix	429:447	The dispersion covering of t-CA in the PHA-g-AA matrix was highly homogeneous as a result of condensation reactions.
24708950	6	19	theme	acetate	1202:1208	arg1	content					1210:1216	cellulose acetate content	1192:1216	cellulose acetate content	1192:1216	The PHA/CA and PHA-g-AA/t-CA composites were more biodegradable than pure PHA, implying a strong connection between cellulose acetate content and biodegradability.
24708950	1	20	theme	poly	214:217	arg1	acetate					174:180	treated (cross-linked) cellulose acetate	141:180	treated (cross-linked) cellulose acetate (t-CA)	141:187	Composites of treated (cross-linked) cellulose acetate (t-CA) and acrylic acid-grafted poly(hydroxyalkanoate) (PHA-g-AA/t-CA) exhibited noticeably superior mechanical properties compared with PHA/CA composites due to greater compatibility between the two components.
24708950	1	20	theme	poly	214:217	arg1	Composites					127:136	Composites	127:136	Composites of treated (cross-linked) cellulose acetate (t-CA) and acrylic acid-grafted poly(hydroxyalkanoate) (PHA-g-AA/t-CA)	127:251	Composites of treated (cross-linked) cellulose acetate (t-CA) and acrylic acid-grafted poly(hydroxyalkanoate) (PHA-g-AA/t-CA) exhibited noticeably superior mechanical properties compared with PHA/CA composites due to greater compatibility between the two components.
24708950	1	20	theme	poly	214:217	arg1	poly					214:217	acrylic acid-grafted poly	193:217	acrylic acid-grafted poly(hydroxyalkanoate) (PHA-g-AA/t-CA)	193:251	Composites of treated (cross-linked) cellulose acetate (t-CA) and acrylic acid-grafted poly(hydroxyalkanoate) (PHA-g-AA/t-CA) exhibited noticeably superior mechanical properties compared with PHA/CA composites due to greater compatibility between the two components.
24708950	1	21	theme	PHA/CA	319:324	arg1	composites					326:335	PHA/CA composites	319:335	PHA/CA composites due to greater compatibility between the two components	319:391	Composites of treated (cross-linked) cellulose acetate (t-CA) and acrylic acid-grafted poly(hydroxyalkanoate) (PHA-g-AA/t-CA) exhibited noticeably superior mechanical properties compared with PHA/CA composites due to greater compatibility between the two components.
24708950	3	22	theme	composites	575:584	arg1	series					565:570	these two series	555:570	these two series of composites	555:584	Human lung fibroblasts (FBs) were seeded on these two series of composites to characterize the biocompatibility properties.
24708950	2	23	theme	covering	409:416	arg1	homogeneous					460:470	homogeneous	460:470	homogeneous	460:470	The dispersion covering of t-CA in the PHA-g-AA matrix was highly homogeneous as a result of condensation reactions.
24708950	2	23	theme	covering	409:416	arg1	dispersion					398:407	The dispersion covering	394:416	The dispersion covering of t-CA in the PHA-g-AA matrix	394:447	The dispersion covering of t-CA in the PHA-g-AA matrix was highly homogeneous as a result of condensation reactions.
24708950	5	24	theme	PHA-g-AA/t-CA	825:837	arg1	higher					843:848	higher	843:848	higher	843:848	The water resistance of PHA-g-AA/t-CA was higher than that of PHA/CA, although the weight loss of both composites buried in Acetobacter pasteurianus (A. pasteurianus) indicated that they were both biodegradable, especially at higher levels of cellulose acetate substitution.
24708950	5	24	theme	PHA-g-AA/t-CA	825:837	arg1	resistance					811:820	The water resistance	801:820	The water resistance of PHA-g-AA/t-CA	801:837	The water resistance of PHA-g-AA/t-CA was higher than that of PHA/CA, although the weight loss of both composites buried in Acetobacter pasteurianus (A. pasteurianus) indicated that they were both biodegradable, especially at higher levels of cellulose acetate substitution.
24708950	5	25	theme	weight	884:889	arg1	loss					891:894	the weight loss	880:894	the weight loss of both composites buried in Acetobacter pasteurianus (A. pasteurianus)	880:966	The water resistance of PHA-g-AA/t-CA was higher than that of PHA/CA, although the weight loss of both composites buried in Acetobacter pasteurianus (A. pasteurianus) indicated that they were both biodegradable, especially at higher levels of cellulose acetate substitution.
24708950	5	26	theme	acetate	1054:1060	arg1	substitution					1062:1073	cellulose acetate substitution	1044:1073	cellulose acetate substitution	1044:1073	The water resistance of PHA-g-AA/t-CA was higher than that of PHA/CA, although the weight loss of both composites buried in Acetobacter pasteurianus (A. pasteurianus) indicated that they were both biodegradable, especially at higher levels of cellulose acetate substitution.
24708950	4	27	theme	higher	705:710	arg1	performance					712:722	higher performance	705:722	higher performance from the PHA/CA series of composites	705:759	In a time-dependent course, the FB proliferation results demonstrated higher performance from the PHA/CA series of composites than from the PHA-g-AA/t-CA composites.
24708950	4	28	theme	proliferation	670:682	arg1	results					684:690	the FB proliferation results	663:690	the FB proliferation results	663:690	In a time-dependent course, the FB proliferation results demonstrated higher performance from the PHA/CA series of composites than from the PHA-g-AA/t-CA composites.
24708950	2	29	theme	reactions	500:508	arg1	result					477:482	a result	475:482	a result of condensation reactions	475:508	The dispersion covering of t-CA in the PHA-g-AA matrix was highly homogeneous as a result of condensation reactions.
24708950	1	30	theme	due	337:339	arg1	composites					326:335	PHA/CA composites	319:335	PHA/CA composites due to greater compatibility between the two components	319:391	Composites of treated (cross-linked) cellulose acetate (t-CA) and acrylic acid-grafted poly(hydroxyalkanoate) (PHA-g-AA/t-CA) exhibited noticeably superior mechanical properties compared with PHA/CA composites due to greater compatibility between the two components.
24708950	5	31	theme	composites	904:913	arg1	loss					891:894	the weight loss	880:894	the weight loss of both composites buried in Acetobacter pasteurianus (A. pasteurianus)	880:966	The water resistance of PHA-g-AA/t-CA was higher than that of PHA/CA, although the weight loss of both composites buried in Acetobacter pasteurianus (A. pasteurianus) indicated that they were both biodegradable, especially at higher levels of cellulose acetate substitution.
24708950	5	32	theme	substitution	1062:1073	arg1	levels					1034:1039	higher levels	1027:1039	higher levels of cellulose acetate substitution	1027:1073	The water resistance of PHA-g-AA/t-CA was higher than that of PHA/CA, although the weight loss of both composites buried in Acetobacter pasteurianus (A. pasteurianus) indicated that they were both biodegradable, especially at higher levels of cellulose acetate substitution.
24708950	2	33	theme	PHA-g-AA	433:440	arg1	matrix					442:447	the PHA-g-AA matrix	429:447	the PHA-g-AA matrix	429:447	The dispersion covering of t-CA in the PHA-g-AA matrix was highly homogeneous as a result of condensation reactions.
24708950	2	34	theme	condensation	487:498	arg1	reactions					500:508	condensation reactions	487:508	condensation reactions	487:508	The dispersion covering of t-CA in the PHA-g-AA matrix was highly homogeneous as a result of condensation reactions.
24708950	5	35	theme	water	805:809	arg1	higher					843:848	higher	843:848	higher	843:848	The water resistance of PHA-g-AA/t-CA was higher than that of PHA/CA, although the weight loss of both composites buried in Acetobacter pasteurianus (A. pasteurianus) indicated that they were both biodegradable, especially at higher levels of cellulose acetate substitution.
24708950	5	35	theme	water	805:809	arg1	resistance					811:820	The water resistance	801:820	The water resistance of PHA-g-AA/t-CA	801:837	The water resistance of PHA-g-AA/t-CA was higher than that of PHA/CA, although the weight loss of both composites buried in Acetobacter pasteurianus (A. pasteurianus) indicated that they were both biodegradable, especially at higher levels of cellulose acetate substitution.
24708950	1	36	theme	treated	141:147	arg1	acetate					174:180	treated (cross-linked) cellulose acetate	141:180	treated (cross-linked) cellulose acetate (t-CA)	141:187	Composites of treated (cross-linked) cellulose acetate (t-CA) and acrylic acid-grafted poly(hydroxyalkanoate) (PHA-g-AA/t-CA) exhibited noticeably superior mechanical properties compared with PHA/CA composites due to greater compatibility between the two components.
24708950	1	36	theme	treated	141:147	arg1	t-CA					183:186	t-CA	183:186	t-CA	183:186	Composites of treated (cross-linked) cellulose acetate (t-CA) and acrylic acid-grafted poly(hydroxyalkanoate) (PHA-g-AA/t-CA) exhibited noticeably superior mechanical properties compared with PHA/CA composites due to greater compatibility between the two components.
24708950	1	37	theme	greater	344:350	arg1	compatibility					352:364	greater compatibility	344:364	greater compatibility between the two components	344:391	Composites of treated (cross-linked) cellulose acetate (t-CA) and acrylic acid-grafted poly(hydroxyalkanoate) (PHA-g-AA/t-CA) exhibited noticeably superior mechanical properties compared with PHA/CA composites due to greater compatibility between the two components.
24708950	3	38	theme	biocompatibility	606:621	arg1	properties					623:632	the biocompatibility properties	602:632	the biocompatibility properties	602:632	Human lung fibroblasts (FBs) were seeded on these two series of composites to characterize the biocompatibility properties.
24708950	5	39	theme	higher	1027:1032	arg1	levels					1034:1039	higher levels	1027:1039	higher levels of cellulose acetate substitution	1027:1073	The water resistance of PHA-g-AA/t-CA was higher than that of PHA/CA, although the weight loss of both composites buried in Acetobacter pasteurianus (A. pasteurianus) indicated that they were both biodegradable, especially at higher levels of cellulose acetate substitution.
24708950	0	40	link	cross-linked	63:74	arg1	composites					115:124	cross-linked cellulose acetate-reinforced polyester composites	63:124	cross-linked cellulose acetate-reinforced polyester composites	63:124	Mechanical properties, biocompatibility, and biodegradation of cross-linked cellulose acetate-reinforced polyester composites.
24708950	3	41	theme	Human	511:515	arg1	fibroblasts					522:532	Human lung fibroblasts	511:532	Human lung fibroblasts (FBs)	511:538	Human lung fibroblasts (FBs) were seeded on these two series of composites to characterize the biocompatibility properties.
24708950	3	41	theme	Human	511:515	arg1	FBs					535:537	FBs	535:537	FBs	535:537	Human lung fibroblasts (FBs) were seeded on these two series of composites to characterize the biocompatibility properties.
24708950	5	42	theme	A.	951:952	arg1	pasteurianus					937:948	Acetobacter pasteurianus	925:948	Acetobacter pasteurianus (A. pasteurianus)	925:966	The water resistance of PHA-g-AA/t-CA was higher than that of PHA/CA, although the weight loss of both composites buried in Acetobacter pasteurianus (A. pasteurianus) indicated that they were both biodegradable, especially at higher levels of cellulose acetate substitution.
24708950	5	42	theme	A.	951:952	arg1	pasteurianus					954:965	A. pasteurianus	951:965	A. pasteurianus	951:965	The water resistance of PHA-g-AA/t-CA was higher than that of PHA/CA, although the weight loss of both composites buried in Acetobacter pasteurianus (A. pasteurianus) indicated that they were both biodegradable, especially at higher levels of cellulose acetate substitution.
24708950	3	43	theme	lung	517:520	arg1	fibroblasts					522:532	Human lung fibroblasts	511:532	Human lung fibroblasts (FBs)	511:538	Human lung fibroblasts (FBs) were seeded on these two series of composites to characterize the biocompatibility properties.
24708950	3	43	theme	lung	517:520	arg1	FBs					535:537	FBs	535:537	FBs	535:537	Human lung fibroblasts (FBs) were seeded on these two series of composites to characterize the biocompatibility properties.
24708950	6	44	theme	strong	1166:1171	arg1	connection					1173:1182	a strong connection	1164:1182	a strong connection between cellulose acetate content and biodegradability	1164:1237	The PHA/CA and PHA-g-AA/t-CA composites were more biodegradable than pure PHA, implying a strong connection between cellulose acetate content and biodegradability.
24708950	0	45	theme	cellulose	76:84	arg1	composites					115:124	cross-linked cellulose acetate-reinforced polyester composites	63:124	cross-linked cellulose acetate-reinforced polyester composites	63:124	Mechanical properties, biocompatibility, and biodegradation of cross-linked cellulose acetate-reinforced polyester composites.
24708950	6	46	theme	PHA/CA	1080:1085	arg1	composites					1105:1114	The PHA/CA and PHA-g-AA/t-CA composites	1076:1114	The PHA/CA and PHA-g-AA/t-CA composites	1076:1114	The PHA/CA and PHA-g-AA/t-CA composites were more biodegradable than pure PHA, implying a strong connection between cellulose acetate content and biodegradability.
24708950	6	46	theme	PHA/CA	1080:1085	arg1	biodegradable					1126:1138	biodegradable	1126:1138	biodegradable	1126:1138	The PHA/CA and PHA-g-AA/t-CA composites were more biodegradable than pure PHA, implying a strong connection between cellulose acetate content and biodegradability.
24708950	1	47	theme	cellulose	164:172	arg1	acetate					174:180	treated (cross-linked) cellulose acetate	141:180	treated (cross-linked) cellulose acetate (t-CA)	141:187	Composites of treated (cross-linked) cellulose acetate (t-CA) and acrylic acid-grafted poly(hydroxyalkanoate) (PHA-g-AA/t-CA) exhibited noticeably superior mechanical properties compared with PHA/CA composites due to greater compatibility between the two components.
24708950	1	47	theme	cellulose	164:172	arg1	t-CA					183:186	t-CA	183:186	t-CA	183:186	Composites of treated (cross-linked) cellulose acetate (t-CA) and acrylic acid-grafted poly(hydroxyalkanoate) (PHA-g-AA/t-CA) exhibited noticeably superior mechanical properties compared with PHA/CA composites due to greater compatibility between the two components.
24708950	0	48	theme	cross-linked	63:74	arg1	composites					115:124	cross-linked cellulose acetate-reinforced polyester composites	63:124	cross-linked cellulose acetate-reinforced polyester composites	63:124	Mechanical properties, biocompatibility, and biodegradation of cross-linked cellulose acetate-reinforced polyester composites.
24708950	5	49	theme	cellulose	1044:1052	arg1	substitution					1062:1073	cellulose acetate substitution	1044:1073	cellulose acetate substitution	1044:1073	The water resistance of PHA-g-AA/t-CA was higher than that of PHA/CA, although the weight loss of both composites buried in Acetobacter pasteurianus (A. pasteurianus) indicated that they were both biodegradable, especially at higher levels of cellulose acetate substitution.
24708950	1	50	theme	acetate	174:180	arg1	acetate					174:180	treated (cross-linked) cellulose acetate	141:180	treated (cross-linked) cellulose acetate (t-CA)	141:187	Composites of treated (cross-linked) cellulose acetate (t-CA) and acrylic acid-grafted poly(hydroxyalkanoate) (PHA-g-AA/t-CA) exhibited noticeably superior mechanical properties compared with PHA/CA composites due to greater compatibility between the two components.
24708950	1	50	theme	acetate	174:180	arg1	Composites					127:136	Composites	127:136	Composites of treated (cross-linked) cellulose acetate (t-CA) and acrylic acid-grafted poly(hydroxyalkanoate) (PHA-g-AA/t-CA)	127:251	Composites of treated (cross-linked) cellulose acetate (t-CA) and acrylic acid-grafted poly(hydroxyalkanoate) (PHA-g-AA/t-CA) exhibited noticeably superior mechanical properties compared with PHA/CA composites due to greater compatibility between the two components.
24708950	1	50	theme	acetate	174:180	arg1	poly					214:217	acrylic acid-grafted poly	193:217	acrylic acid-grafted poly(hydroxyalkanoate) (PHA-g-AA/t-CA)	193:251	Composites of treated (cross-linked) cellulose acetate (t-CA) and acrylic acid-grafted poly(hydroxyalkanoate) (PHA-g-AA/t-CA) exhibited noticeably superior mechanical properties compared with PHA/CA composites due to greater compatibility between the two components.
24708950	4	51	from	series	740:745	arg1	performance					712:722	higher performance	705:722	higher performance from the PHA/CA series of composites	705:759	In a time-dependent course, the FB proliferation results demonstrated higher performance from the PHA/CA series of composites than from the PHA-g-AA/t-CA composites.
24708950	4	52	theme	time-dependent	640:653	arg1	course					655:660	a time-dependent course	638:660	a time-dependent course	638:660	In a time-dependent course, the FB proliferation results demonstrated higher performance from the PHA/CA series of composites than from the PHA-g-AA/t-CA composites.
25919933	1	0	theme	S-sludge	160:167	arg1	floc					170:173	suspended sludge (S-sludge) floc	142:173	suspended sludge (S-sludge) floc	142:173	Biofilm and suspended sludge (S-sludge) floc exhibit distinct physicochemical properties and process performances in an integrated fixed-film and suspended growth sequencing batch reactor.
25919933	8	1	from	aggregates	1052:1061	arg1	compositions					1026:1037	the monosaccharide compositions	1007:1037	the monosaccharide compositions from the two aggregates	1007:1061	In addition, the monosaccharide compositions from the two aggregates were various.
25919933	8	1	from	aggregates	1052:1061	arg1	various					1068:1074	various	1068:1074	various	1068:1074	In addition, the monosaccharide compositions from the two aggregates were various.
25919933	1	2	theme	suspended	276:284	arg1	reactor					310:316	suspended growth sequencing batch reactor	276:316	suspended growth sequencing batch reactor	276:316	Biofilm and suspended sludge (S-sludge) floc exhibit distinct physicochemical properties and process performances in an integrated fixed-film and suspended growth sequencing batch reactor.
25919933	7	3	from	activity	929:936	arg1	S-sludge					945:952	the S-sludge	941:952	the S-sludge	941:952	In contrast, more extracellular proteins related to catalytic activity in the S-sludge could decrease the compactness of floc.
25919933	1	4	theme	growth	286:291	arg1	reactor					310:316	suspended growth sequencing batch reactor	276:316	suspended growth sequencing batch reactor	276:316	Biofilm and suspended sludge (S-sludge) floc exhibit distinct physicochemical properties and process performances in an integrated fixed-film and suspended growth sequencing batch reactor.
25919933	9	5	theme	architecture	1130:1141	arg1	diversities					1115:1125	the diversities	1111:1125	the diversities of architecture and biochemical process between the two aggregates	1111:1192	These results could elucidate how the diversities of architecture and biochemical process between the two aggregates occurred.
25919933	1	6	theme	sequencing	293:302	arg1	reactor					310:316	suspended growth sequencing batch reactor	276:316	suspended growth sequencing batch reactor	276:316	Biofilm and suspended sludge (S-sludge) floc exhibit distinct physicochemical properties and process performances in an integrated fixed-film and suspended growth sequencing batch reactor.
25919933	5	7	theme	microbial	675:683	arg1	backbone					719:726	the backbone	715:726	the backbone of aggregates	715:740	Furthermore, many microbial cells in the biofilm acted as the backbone of aggregates and maintained the structure stability.
25919933	5	7	theme	microbial	675:683	arg1	cells					685:689	many microbial cells	670:689	many microbial cells in the biofilm	670:704	Furthermore, many microbial cells in the biofilm acted as the backbone of aggregates and maintained the structure stability.
25919933	5	7	theme	microbial	675:683	arg1	stability					771:779	the structure stability	757:779	the structure stability	757:779	Furthermore, many microbial cells in the biofilm acted as the backbone of aggregates and maintained the structure stability.
25919933	5	8	theme	many	670:673	arg1	backbone					719:726	the backbone	715:726	the backbone of aggregates	715:740	Furthermore, many microbial cells in the biofilm acted as the backbone of aggregates and maintained the structure stability.
25919933	5	8	theme	many	670:673	arg1	cells					685:689	many microbial cells	670:689	many microbial cells in the biofilm	670:704	Furthermore, many microbial cells in the biofilm acted as the backbone of aggregates and maintained the structure stability.
25919933	5	8	theme	many	670:673	arg1	stability					771:779	the structure stability	757:779	the structure stability	757:779	Furthermore, many microbial cells in the biofilm acted as the backbone of aggregates and maintained the structure stability.
25919933	1	9	theme	distinct	183:190	arg1	properties					208:217	distinct physicochemical properties	183:217	distinct physicochemical properties	183:217	Biofilm and suspended sludge (S-sludge) floc exhibit distinct physicochemical properties and process performances in an integrated fixed-film and suspended growth sequencing batch reactor.
25919933	3	10	theme	microbial	469:477	arg1	communities					479:489	microbial communities	469:489	microbial communities	469:489	Current work evaluated the diversity of morphologies, microbial communities, extracellular proteins and polysaccharides between the biofilm and S-sludge.
25919933	0	11	theme	suspended	112:120	arg1	sludge					122:127	suspended sludge	112:127	suspended sludge	112:127	Microbial communities, extracellular proteomics and polysaccharides: A comparative investigation on biofilm and suspended sludge.
25919933	3	12	dep	biofilm	547:553	arg1	the					543:545	the	543:545	the	543:545	Current work evaluated the diversity of morphologies, microbial communities, extracellular proteins and polysaccharides between the biofilm and S-sludge.
25919933	9	13	theme	biochemical	1147:1157	arg1	process					1159:1165	biochemical process	1147:1165	biochemical process	1147:1165	These results could elucidate how the diversities of architecture and biochemical process between the two aggregates occurred.
25919933	3	14	theme	communities	479:489	arg1	diversity					442:450	the diversity	438:450	the diversity of morphologies, microbial communities, extracellular proteins and polysaccharides between the biofilm and S-sludge	438:566	Current work evaluated the diversity of morphologies, microbial communities, extracellular proteins and polysaccharides between the biofilm and S-sludge.
25919933	3	15	theme	Current	415:421	arg1	work					423:426	Current work	415:426	Current work	415:426	Current work evaluated the diversity of morphologies, microbial communities, extracellular proteins and polysaccharides between the biofilm and S-sludge.
25919933	7	16	theme	related	908:914	arg1	proteins					899:906	more extracellular proteins	880:906	more extracellular proteins related to catalytic activity in the S-sludge	880:952	In contrast, more extracellular proteins related to catalytic activity in the S-sludge could decrease the compactness of floc.
25919933	3	17	theme	morphologies	455:466	arg1	diversity					442:450	the diversity	438:450	the diversity of morphologies, microbial communities, extracellular proteins and polysaccharides between the biofilm and S-sludge	438:566	Current work evaluated the diversity of morphologies, microbial communities, extracellular proteins and polysaccharides between the biofilm and S-sludge.
25919933	0	18	theme	Microbial	0:8	arg1	communities					10:20	Microbial communities	0:20	Microbial communities	0:20	Microbial communities, extracellular proteomics and polysaccharides: A comparative investigation on biofilm and suspended sludge.
25919933	1	19	theme	batch	304:308	arg1	reactor					310:316	suspended growth sequencing batch reactor	276:316	suspended growth sequencing batch reactor	276:316	Biofilm and suspended sludge (S-sludge) floc exhibit distinct physicochemical properties and process performances in an integrated fixed-film and suspended growth sequencing batch reactor.
25919933	7	20	theme	more	880:883	arg1	proteins					899:906	more extracellular proteins	880:906	more extracellular proteins related to catalytic activity in the S-sludge	880:952	In contrast, more extracellular proteins related to catalytic activity in the S-sludge could decrease the compactness of floc.
25919933	1	21	theme	physicochemical	192:206	arg1	properties					208:217	distinct physicochemical properties	183:217	distinct physicochemical properties	183:217	Biofilm and suspended sludge (S-sludge) floc exhibit distinct physicochemical properties and process performances in an integrated fixed-film and suspended growth sequencing batch reactor.
25919933	0	22	theme	extracellular	23:35	arg1	proteomics					37:46	extracellular proteomics	23:46	extracellular proteomics	23:46	Microbial communities, extracellular proteomics and polysaccharides: A comparative investigation on biofilm and suspended sludge.
25919933	6	23	theme	cell	852:855	arg1	adhesion					857:864	the cell adhesion	848:864	the cell adhesion	848:864	An extracellular protein observed only in the biofilm can promote the cell adhesion.
25919933	7	24	theme	extracellular	885:897	arg1	proteins					899:906	more extracellular proteins	880:906	more extracellular proteins related to catalytic activity in the S-sludge	880:952	In contrast, more extracellular proteins related to catalytic activity in the S-sludge could decrease the compactness of floc.
25919933	3	25	theme	proteins	506:513	arg1	diversity					442:450	the diversity	438:450	the diversity of morphologies, microbial communities, extracellular proteins and polysaccharides between the biofilm and S-sludge	438:566	Current work evaluated the diversity of morphologies, microbial communities, extracellular proteins and polysaccharides between the biofilm and S-sludge.
25919933	5	26	from	cells	685:689	arg1	biofilm					698:704	the biofilm	694:704	the biofilm	694:704	Furthermore, many microbial cells in the biofilm acted as the backbone of aggregates and maintained the structure stability.
25919933	5	27	theme	structure	761:769	arg1	backbone					719:726	the backbone	715:726	the backbone of aggregates	715:740	Furthermore, many microbial cells in the biofilm acted as the backbone of aggregates and maintained the structure stability.
25919933	5	27	theme	structure	761:769	arg1	cells					685:689	many microbial cells	670:689	many microbial cells in the biofilm	670:704	Furthermore, many microbial cells in the biofilm acted as the backbone of aggregates and maintained the structure stability.
25919933	5	27	theme	structure	761:769	arg1	stability					771:779	the structure stability	757:779	the structure stability	757:779	Furthermore, many microbial cells in the biofilm acted as the backbone of aggregates and maintained the structure stability.
25919933	0	28	from	investigation	83:95	arg1	biofilm					100:106	biofilm	100:106	biofilm	100:106	Microbial communities, extracellular proteomics and polysaccharides: A comparative investigation on biofilm and suspended sludge.
25919933	0	28	from	investigation	83:95	arg1	sludge					122:127	suspended sludge	112:127	suspended sludge	112:127	Microbial communities, extracellular proteomics and polysaccharides: A comparative investigation on biofilm and suspended sludge.
25919933	9	29	theme	process	1159:1165	arg1	diversities					1115:1125	the diversities	1111:1125	the diversities of architecture and biochemical process between the two aggregates	1111:1192	These results could elucidate how the diversities of architecture and biochemical process between the two aggregates occurred.
25919933	3	30	theme	extracellular	492:504	arg1	proteins					506:513	extracellular proteins	492:513	extracellular proteins	492:513	Current work evaluated the diversity of morphologies, microbial communities, extracellular proteins and polysaccharides between the biofilm and S-sludge.
25919933	6	31	located	observed	807:814	arg1	biofilm					828:834	the biofilm	824:834	the biofilm	824:834	An extracellular protein observed only in the biofilm can promote the cell adhesion.
25919933	6	31	located	observed	807:814	arg2	protein					799:805	An extracellular protein	782:805	An extracellular protein observed only in the biofilm	782:834	An extracellular protein observed only in the biofilm can promote the cell adhesion.
25919933	7	32	theme	floc	988:991	arg1	compactness					973:983	the compactness	969:983	the compactness of floc	969:991	In contrast, more extracellular proteins related to catalytic activity in the S-sludge could decrease the compactness of floc.
25919933	5	33	theme	aggregates	731:740	arg1	backbone					719:726	the backbone	715:726	the backbone of aggregates	715:740	Furthermore, many microbial cells in the biofilm acted as the backbone of aggregates and maintained the structure stability.
25919933	5	33	theme	aggregates	731:740	arg1	cells					685:689	many microbial cells	670:689	many microbial cells in the biofilm	670:704	Furthermore, many microbial cells in the biofilm acted as the backbone of aggregates and maintained the structure stability.
25919933	5	33	theme	aggregates	731:740	arg1	stability					771:779	the structure stability	757:779	the structure stability	757:779	Furthermore, many microbial cells in the biofilm acted as the backbone of aggregates and maintained the structure stability.
25919933	3	34	theme	polysaccharides	519:533	arg1	diversity					442:450	the diversity	438:450	the diversity of morphologies, microbial communities, extracellular proteins and polysaccharides between the biofilm and S-sludge	438:566	Current work evaluated the diversity of morphologies, microbial communities, extracellular proteins and polysaccharides between the biofilm and S-sludge.
25919933	7	35	theme	catalytic	919:927	arg1	activity					929:936	catalytic activity	919:936	catalytic activity in the S-sludge	919:952	In contrast, more extracellular proteins related to catalytic activity in the S-sludge could decrease the compactness of floc.
25919933	1	36	theme	suspended	142:150	arg1	floc					170:173	suspended sludge (S-sludge) floc	142:173	suspended sludge (S-sludge) floc	142:173	Biofilm and suspended sludge (S-sludge) floc exhibit distinct physicochemical properties and process performances in an integrated fixed-film and suspended growth sequencing batch reactor.
25919933	0	37	theme	comparative	71:81	arg1	investigation					83:95	A comparative investigation	69:95	Microbial communities, extracellular proteomics and polysaccharides: A comparative investigation on biofilm and suspended sludge.	0:128	Microbial communities, extracellular proteomics and polysaccharides: A comparative investigation on biofilm and suspended sludge.
25919933	8	38	from	addition	997:1004	arg1	compositions					1026:1037	the monosaccharide compositions	1007:1037	the monosaccharide compositions from the two aggregates	1007:1061	In addition, the monosaccharide compositions from the two aggregates were various.
25919933	8	38	from	addition	997:1004	arg1	various					1068:1074	various	1068:1074	various	1068:1074	In addition, the monosaccharide compositions from the two aggregates were various.
25919933	0	39	dep	communities	10:20	arg1	investigation					83:95	A comparative investigation	69:95	Microbial communities, extracellular proteomics and polysaccharides: A comparative investigation on biofilm and suspended sludge.	0:128	Microbial communities, extracellular proteomics and polysaccharides: A comparative investigation on biofilm and suspended sludge.
25919933	6	40	theme	extracellular	785:797	arg1	protein					799:805	An extracellular protein	782:805	An extracellular protein observed only in the biofilm	782:834	An extracellular protein observed only in the biofilm can promote the cell adhesion.
25919933	1	41	theme	sludge	152:157	arg1	floc					170:173	suspended sludge (S-sludge) floc	142:173	suspended sludge (S-sludge) floc	142:173	Biofilm and suspended sludge (S-sludge) floc exhibit distinct physicochemical properties and process performances in an integrated fixed-film and suspended growth sequencing batch reactor.
25919933	1	42	theme	integrated	250:259	arg1	fixed-film					261:270	an integrated fixed-film	247:270	an integrated fixed-film	247:270	Biofilm and suspended sludge (S-sludge) floc exhibit distinct physicochemical properties and process performances in an integrated fixed-film and suspended growth sequencing batch reactor.
25919933	8	43	theme	monosaccharide	1011:1024	arg1	compositions					1026:1037	the monosaccharide compositions	1007:1037	the monosaccharide compositions from the two aggregates	1007:1061	In addition, the monosaccharide compositions from the two aggregates were various.
25919933	8	43	theme	monosaccharide	1011:1024	arg1	various					1068:1074	various	1068:1074	various	1068:1074	In addition, the monosaccharide compositions from the two aggregates were various.
25919933	8	44	from	various	1068:1074	arg1	addition					997:1004	addition	997:1004	addition	997:1004	In addition, the monosaccharide compositions from the two aggregates were various.
25133663	2	0	with	superdisintegrant	392:408	arg1	properties					423:432	binding properties	415:432	binding properties	415:432	The objective of this study was to evaluate the dissolution performance of amorphous solid dispersions containing a dispersed superdisintegrant with binding properties.
25133663	5	1	theme	similar	888:894	arg1	performance					876:886	non-sink dissolution performance	855:886	non-sink dissolution performance similar to milled intermediate	855:917	Tablets containing intra-dispersion NBD were found to exhibit non-sink dissolution performance similar to milled intermediate, demonstrating excellent disintegration characteristics.
25133663	2	2	theme	dispersions	357:367	arg1	performance					326:336	the dissolution performance	310:336	the dissolution performance of amorphous solid dispersions containing a dispersed superdisintegrant with binding properties	310:432	The objective of this study was to evaluate the dissolution performance of amorphous solid dispersions containing a dispersed superdisintegrant with binding properties.
25133663	6	3	theme	less	1061:1064	arg1	NIF					1066:1068	significantly less NIF	1047:1068	significantly less NIF	1047:1068	Conversely, tablets without intra-dispersion NBD were found to release significantly less NIF during dissolution analysis due to particle agglomeration.
25133663	3	4	theme	cellulose	649:657	arg1	Disintegrants					671:683	New Binder Disintegrants	660:683	New Binder Disintegrants; NBD-grade	660:694	KinetiSol® dispersing was used to thermally process hypromellose acetate succinate-based compositions containing the drug substance nifedipine (NIF) and a highly compressible grade of low-substituted hydroxypropyl cellulose (New Binder Disintegrants; NBD-grade).
25133663	3	4	theme	cellulose	649:657	arg1	NIF					579:581	NIF	579:581	NIF	579:581	KinetiSol® dispersing was used to thermally process hypromellose acetate succinate-based compositions containing the drug substance nifedipine (NIF) and a highly compressible grade of low-substituted hydroxypropyl cellulose (New Binder Disintegrants; NBD-grade).
25133663	3	4	theme	cellulose	649:657	arg1	grade					610:614	a highly compressible grade	588:614	a highly compressible grade of low-substituted hydroxypropyl cellulose (New Binder Disintegrants; NBD-grade)	588:695	KinetiSol® dispersing was used to thermally process hypromellose acetate succinate-based compositions containing the drug substance nifedipine (NIF) and a highly compressible grade of low-substituted hydroxypropyl cellulose (New Binder Disintegrants; NBD-grade).
25133663	3	4	theme	cellulose	649:657	arg1	nifedipine					567:576	the drug substance nifedipine	548:576	the drug substance nifedipine (NIF)	548:582	KinetiSol® dispersing was used to thermally process hypromellose acetate succinate-based compositions containing the drug substance nifedipine (NIF) and a highly compressible grade of low-substituted hydroxypropyl cellulose (New Binder Disintegrants; NBD-grade).
25133663	3	5	theme	compressible	597:608	arg1	Disintegrants					671:683	New Binder Disintegrants	660:683	New Binder Disintegrants; NBD-grade	660:694	KinetiSol® dispersing was used to thermally process hypromellose acetate succinate-based compositions containing the drug substance nifedipine (NIF) and a highly compressible grade of low-substituted hydroxypropyl cellulose (New Binder Disintegrants; NBD-grade).
25133663	3	5	theme	compressible	597:608	arg1	grade					610:614	a highly compressible grade	588:614	a highly compressible grade of low-substituted hydroxypropyl cellulose (New Binder Disintegrants; NBD-grade)	588:695	KinetiSol® dispersing was used to thermally process hypromellose acetate succinate-based compositions containing the drug substance nifedipine (NIF) and a highly compressible grade of low-substituted hydroxypropyl cellulose (New Binder Disintegrants; NBD-grade).
25133663	3	6	theme	low-substituted	619:633	arg1	cellulose					649:657	low-substituted hydroxypropyl cellulose	619:657	low-substituted hydroxypropyl cellulose	619:657	KinetiSol® dispersing was used to thermally process hypromellose acetate succinate-based compositions containing the drug substance nifedipine (NIF) and a highly compressible grade of low-substituted hydroxypropyl cellulose (New Binder Disintegrants; NBD-grade).
25133663	3	7	used	used	461:464	arg2	KinetiSol®					435:444	KinetiSol®	435:444	KinetiSol® dispersing	435:455	KinetiSol® dispersing was used to thermally process hypromellose acetate succinate-based compositions containing the drug substance nifedipine (NIF) and a highly compressible grade of low-substituted hydroxypropyl cellulose (New Binder Disintegrants; NBD-grade).
25133663	2	8	theme	study	288:292	arg1	objective					270:278	The objective	266:278	The objective of this study	266:292	The objective of this study was to evaluate the dissolution performance of amorphous solid dispersions containing a dispersed superdisintegrant with binding properties.
25133663	3	9	contain	containing	537:546	arg2	grade					610:614	a highly compressible grade	588:614	a highly compressible grade of low-substituted hydroxypropyl cellulose (New Binder Disintegrants; NBD-grade)	588:695	KinetiSol® dispersing was used to thermally process hypromellose acetate succinate-based compositions containing the drug substance nifedipine (NIF) and a highly compressible grade of low-substituted hydroxypropyl cellulose (New Binder Disintegrants; NBD-grade).
25133663	3	9	contain	containing	537:546	arg2	NIF					579:581	NIF	579:581	NIF	579:581	KinetiSol® dispersing was used to thermally process hypromellose acetate succinate-based compositions containing the drug substance nifedipine (NIF) and a highly compressible grade of low-substituted hydroxypropyl cellulose (New Binder Disintegrants; NBD-grade).
25133663	3	9	contain	containing	537:546	arg2	Disintegrants					671:683	New Binder Disintegrants	660:683	New Binder Disintegrants; NBD-grade	660:694	KinetiSol® dispersing was used to thermally process hypromellose acetate succinate-based compositions containing the drug substance nifedipine (NIF) and a highly compressible grade of low-substituted hydroxypropyl cellulose (New Binder Disintegrants; NBD-grade).
25133663	3	9	contain	containing	537:546	arg2	nifedipine					567:576	the drug substance nifedipine	548:576	the drug substance nifedipine (NIF)	548:582	KinetiSol® dispersing was used to thermally process hypromellose acetate succinate-based compositions containing the drug substance nifedipine (NIF) and a highly compressible grade of low-substituted hydroxypropyl cellulose (New Binder Disintegrants; NBD-grade).
25133663	3	9	contain	containing	537:546	arg1	compositions					524:535	hypromellose acetate succinate-based compositions	487:535	hypromellose acetate succinate-based compositions containing the drug substance nifedipine (NIF) and a highly compressible grade of low-substituted hydroxypropyl cellulose (New Binder Disintegrants; NBD-grade)	487:695	KinetiSol® dispersing was used to thermally process hypromellose acetate succinate-based compositions containing the drug substance nifedipine (NIF) and a highly compressible grade of low-substituted hydroxypropyl cellulose (New Binder Disintegrants; NBD-grade).
25133663	3	10	theme	drug	552:555	arg1	NIF					579:581	NIF	579:581	NIF	579:581	KinetiSol® dispersing was used to thermally process hypromellose acetate succinate-based compositions containing the drug substance nifedipine (NIF) and a highly compressible grade of low-substituted hydroxypropyl cellulose (New Binder Disintegrants; NBD-grade).
25133663	3	10	theme	drug	552:555	arg1	nifedipine					567:576	the drug substance nifedipine	548:576	the drug substance nifedipine (NIF)	548:582	KinetiSol® dispersing was used to thermally process hypromellose acetate succinate-based compositions containing the drug substance nifedipine (NIF) and a highly compressible grade of low-substituted hydroxypropyl cellulose (New Binder Disintegrants; NBD-grade).
25133663	2	11	theme	binding	415:421	arg1	properties					423:432	binding properties	415:432	binding properties	415:432	The objective of this study was to evaluate the dissolution performance of amorphous solid dispersions containing a dispersed superdisintegrant with binding properties.
25133663	7	12	theme	NBD	1232:1234	arg1	level					1206:1210	the level	1202:1210	the level of intra-dispersion NBD	1202:1234	It was determined that compressibility and particle wetting increased as the level of intra-dispersion NBD increased.
25133663	2	13	theme	dissolution	314:324	arg1	performance					326:336	the dissolution performance	310:336	the dissolution performance of amorphous solid dispersions containing a dispersed superdisintegrant with binding properties	310:432	The objective of this study was to evaluate the dissolution performance of amorphous solid dispersions containing a dispersed superdisintegrant with binding properties.
25133663	2	14	contain	containing	369:378	arg2	superdisintegrant					392:408	a dispersed superdisintegrant	380:408	a dispersed superdisintegrant with binding properties	380:432	The objective of this study was to evaluate the dissolution performance of amorphous solid dispersions containing a dispersed superdisintegrant with binding properties.
25133663	2	14	contain	containing	369:378	arg1	dispersions					357:367	amorphous solid dispersions	341:367	amorphous solid dispersions containing a dispersed superdisintegrant with binding properties	341:432	The objective of this study was to evaluate the dissolution performance of amorphous solid dispersions containing a dispersed superdisintegrant with binding properties.
25133663	1	15	theme	little	187:192	arg1	consideration					194:206	little consideration	187:206	little consideration	187:206	While the use of amorphous solid dispersions to improve aqueous solubility is well documented, little consideration has traditionally been given to the finished dosage form.
25133663	5	16	theme	excellent	934:942	arg1	characteristics					959:973	excellent disintegration characteristics	934:973	excellent disintegration characteristics	934:973	Tablets containing intra-dispersion NBD were found to exhibit non-sink dissolution performance similar to milled intermediate, demonstrating excellent disintegration characteristics.
25133663	3	17	theme	hydroxypropyl	635:647	arg1	cellulose					649:657	low-substituted hydroxypropyl cellulose	619:657	low-substituted hydroxypropyl cellulose	619:657	KinetiSol® dispersing was used to thermally process hypromellose acetate succinate-based compositions containing the drug substance nifedipine (NIF) and a highly compressible grade of low-substituted hydroxypropyl cellulose (New Binder Disintegrants; NBD-grade).
25133663	0	18	theme	low-substituted	21:35	arg1	cellulose					51:59	low-substituted hydroxypropyl cellulose	21:59	low-substituted hydroxypropyl cellulose	21:59	The incorporation of low-substituted hydroxypropyl cellulose into solid dispersion systems.
25133663	3	19	theme	succinate-based	508:522	arg1	compositions					524:535	hypromellose acetate succinate-based compositions	487:535	hypromellose acetate succinate-based compositions containing the drug substance nifedipine (NIF) and a highly compressible grade of low-substituted hydroxypropyl cellulose (New Binder Disintegrants; NBD-grade)	487:695	KinetiSol® dispersing was used to thermally process hypromellose acetate succinate-based compositions containing the drug substance nifedipine (NIF) and a highly compressible grade of low-substituted hydroxypropyl cellulose (New Binder Disintegrants; NBD-grade).
25133663	5	20	contain	containing	801:810	arg2	NBD					829:831	intra-dispersion NBD	812:831	intra-dispersion NBD	812:831	Tablets containing intra-dispersion NBD were found to exhibit non-sink dissolution performance similar to milled intermediate, demonstrating excellent disintegration characteristics.
25133663	5	20	contain	containing	801:810	arg1	Tablets					793:799	Tablets	793:799	Tablets containing intra-dispersion NBD	793:831	Tablets containing intra-dispersion NBD were found to exhibit non-sink dissolution performance similar to milled intermediate, demonstrating excellent disintegration characteristics.
25133663	1	21	theme	amorphous	109:117	arg1	dispersions					125:135	amorphous solid dispersions	109:135	amorphous solid dispersions	109:135	While the use of amorphous solid dispersions to improve aqueous solubility is well documented, little consideration has traditionally been given to the finished dosage form.
25133663	1	22	theme	aqueous	148:154	arg1	solubility					156:165	aqueous solubility	148:165	aqueous solubility	148:165	While the use of amorphous solid dispersions to improve aqueous solubility is well documented, little consideration has traditionally been given to the finished dosage form.
25133663	0	23	theme	cellulose	51:59	arg1	incorporation					4:16	The incorporation	0:16	The incorporation of low-substituted hydroxypropyl cellulose into solid dispersion systems	0:89	The incorporation of low-substituted hydroxypropyl cellulose into solid dispersion systems.
25133663	2	24	theme	amorphous	341:349	arg1	dispersions					357:367	amorphous solid dispersions	341:367	amorphous solid dispersions containing a dispersed superdisintegrant with binding properties	341:432	The objective of this study was to evaluate the dissolution performance of amorphous solid dispersions containing a dispersed superdisintegrant with binding properties.
25133663	5	25	theme	intra-dispersion	812:827	arg1	NBD					829:831	intra-dispersion NBD	812:831	intra-dispersion NBD	812:831	Tablets containing intra-dispersion NBD were found to exhibit non-sink dissolution performance similar to milled intermediate, demonstrating excellent disintegration characteristics.
25133663	3	26	theme	New	660:662	arg1	grade					610:614	a highly compressible grade	588:614	a highly compressible grade of low-substituted hydroxypropyl cellulose (New Binder Disintegrants; NBD-grade)	588:695	KinetiSol® dispersing was used to thermally process hypromellose acetate succinate-based compositions containing the drug substance nifedipine (NIF) and a highly compressible grade of low-substituted hydroxypropyl cellulose (New Binder Disintegrants; NBD-grade).
25133663	3	26	theme	New	660:662	arg1	Disintegrants					671:683	New Binder Disintegrants	660:683	New Binder Disintegrants; NBD-grade	660:694	KinetiSol® dispersing was used to thermally process hypromellose acetate succinate-based compositions containing the drug substance nifedipine (NIF) and a highly compressible grade of low-substituted hydroxypropyl cellulose (New Binder Disintegrants; NBD-grade).
25133663	3	27	theme	hypromellose	487:498	arg1	compositions					524:535	hypromellose acetate succinate-based compositions	487:535	hypromellose acetate succinate-based compositions containing the drug substance nifedipine (NIF) and a highly compressible grade of low-substituted hydroxypropyl cellulose (New Binder Disintegrants; NBD-grade)	487:695	KinetiSol® dispersing was used to thermally process hypromellose acetate succinate-based compositions containing the drug substance nifedipine (NIF) and a highly compressible grade of low-substituted hydroxypropyl cellulose (New Binder Disintegrants; NBD-grade).
25133663	0	28	theme	hydroxypropyl	37:49	arg1	cellulose					51:59	low-substituted hydroxypropyl cellulose	21:59	low-substituted hydroxypropyl cellulose	21:59	The incorporation of low-substituted hydroxypropyl cellulose into solid dispersion systems.
25133663	6	29	theme	particle	1105:1112	arg1	agglomeration					1114:1126	particle agglomeration	1105:1126	particle agglomeration	1105:1126	Conversely, tablets without intra-dispersion NBD were found to release significantly less NIF during dissolution analysis due to particle agglomeration.
25133663	5	30	theme	milled	899:904	arg1	intermediate					906:917	milled intermediate	899:917	milled intermediate	899:917	Tablets containing intra-dispersion NBD were found to exhibit non-sink dissolution performance similar to milled intermediate, demonstrating excellent disintegration characteristics.
25133663	6	31	theme	intra-dispersion	1004:1019	arg1	NBD					1021:1023	intra-dispersion NBD	1004:1023	intra-dispersion NBD	1004:1023	Conversely, tablets without intra-dispersion NBD were found to release significantly less NIF during dissolution analysis due to particle agglomeration.
25133663	3	32	theme	Binder	664:669	arg1	grade					610:614	a highly compressible grade	588:614	a highly compressible grade of low-substituted hydroxypropyl cellulose (New Binder Disintegrants; NBD-grade)	588:695	KinetiSol® dispersing was used to thermally process hypromellose acetate succinate-based compositions containing the drug substance nifedipine (NIF) and a highly compressible grade of low-substituted hydroxypropyl cellulose (New Binder Disintegrants; NBD-grade).
25133663	3	32	theme	Binder	664:669	arg1	Disintegrants					671:683	New Binder Disintegrants	660:683	New Binder Disintegrants; NBD-grade	660:694	KinetiSol® dispersing was used to thermally process hypromellose acetate succinate-based compositions containing the drug substance nifedipine (NIF) and a highly compressible grade of low-substituted hydroxypropyl cellulose (New Binder Disintegrants; NBD-grade).
25133663	3	33	theme	acetate	500:506	arg1	compositions					524:535	hypromellose acetate succinate-based compositions	487:535	hypromellose acetate succinate-based compositions containing the drug substance nifedipine (NIF) and a highly compressible grade of low-substituted hydroxypropyl cellulose (New Binder Disintegrants; NBD-grade)	487:695	KinetiSol® dispersing was used to thermally process hypromellose acetate succinate-based compositions containing the drug substance nifedipine (NIF) and a highly compressible grade of low-substituted hydroxypropyl cellulose (New Binder Disintegrants; NBD-grade).
25133663	0	34	theme	solid	66:70	arg1	systems					83:89	solid dispersion systems	66:89	solid dispersion systems	66:89	The incorporation of low-substituted hydroxypropyl cellulose into solid dispersion systems.
25133663	5	35	theme	dissolution	864:874	arg1	performance					876:886	non-sink dissolution performance	855:886	non-sink dissolution performance similar to milled intermediate	855:917	Tablets containing intra-dispersion NBD were found to exhibit non-sink dissolution performance similar to milled intermediate, demonstrating excellent disintegration characteristics.
25133663	2	36	theme	solid	351:355	arg1	dispersions					357:367	amorphous solid dispersions	341:367	amorphous solid dispersions containing a dispersed superdisintegrant with binding properties	341:432	The objective of this study was to evaluate the dissolution performance of amorphous solid dispersions containing a dispersed superdisintegrant with binding properties.
25133663	1	37	theme	solid	119:123	arg1	dispersions					125:135	amorphous solid dispersions	109:135	amorphous solid dispersions	109:135	While the use of amorphous solid dispersions to improve aqueous solubility is well documented, little consideration has traditionally been given to the finished dosage form.
25133663	7	38	theme	intra-dispersion	1215:1230	arg1	NBD					1232:1234	intra-dispersion NBD	1215:1234	intra-dispersion NBD	1215:1234	It was determined that compressibility and particle wetting increased as the level of intra-dispersion NBD increased.
25133663	5	39	theme	disintegration	944:957	arg1	characteristics					959:973	excellent disintegration characteristics	934:973	excellent disintegration characteristics	934:973	Tablets containing intra-dispersion NBD were found to exhibit non-sink dissolution performance similar to milled intermediate, demonstrating excellent disintegration characteristics.
25133663	1	40	theme	dispersions	125:135	arg1	use					102:104	the use	98:104	the use of amorphous solid dispersions to improve aqueous solubility	98:165	While the use of amorphous solid dispersions to improve aqueous solubility is well documented, little consideration has traditionally been given to the finished dosage form.
25133663	5	41	theme	non-sink	855:862	arg1	performance					876:886	non-sink dissolution performance	855:886	non-sink dissolution performance similar to milled intermediate	855:917	Tablets containing intra-dispersion NBD were found to exhibit non-sink dissolution performance similar to milled intermediate, demonstrating excellent disintegration characteristics.
25133663	3	42	dep	Disintegrants	671:683	arg1	NBD-grade					686:694	NBD-grade	686:694	New Binder Disintegrants; NBD-grade	660:694	KinetiSol® dispersing was used to thermally process hypromellose acetate succinate-based compositions containing the drug substance nifedipine (NIF) and a highly compressible grade of low-substituted hydroxypropyl cellulose (New Binder Disintegrants; NBD-grade).
25133663	2	43	theme	dispersed	382:390	arg1	superdisintegrant					392:408	a dispersed superdisintegrant	380:408	a dispersed superdisintegrant with binding properties	380:432	The objective of this study was to evaluate the dissolution performance of amorphous solid dispersions containing a dispersed superdisintegrant with binding properties.
25133663	1	44	theme	finished	244:251	arg1	form					260:263	the finished dosage form	240:263	the finished dosage form	240:263	While the use of amorphous solid dispersions to improve aqueous solubility is well documented, little consideration has traditionally been given to the finished dosage form.
25133663	0	45	theme	dispersion	72:81	arg1	systems					83:89	solid dispersion systems	66:89	solid dispersion systems	66:89	The incorporation of low-substituted hydroxypropyl cellulose into solid dispersion systems.
25133663	6	46	theme	dissolution	1077:1087	arg1	analysis					1089:1096	dissolution analysis	1077:1096	dissolution analysis due to particle agglomeration	1077:1126	Conversely, tablets without intra-dispersion NBD were found to release significantly less NIF during dissolution analysis due to particle agglomeration.
25133663	4	47	theme	Solid-state	698:708	arg1	analysis					710:717	Solid-state analysis	698:717	Solid-state analysis	698:717	Solid-state analysis demonstrated that compositions were rendered amorphous during processing.
25133663	3	48	theme	substance	557:565	arg1	NIF					579:581	NIF	579:581	NIF	579:581	KinetiSol® dispersing was used to thermally process hypromellose acetate succinate-based compositions containing the drug substance nifedipine (NIF) and a highly compressible grade of low-substituted hydroxypropyl cellulose (New Binder Disintegrants; NBD-grade).
25133663	3	48	theme	substance	557:565	arg1	nifedipine					567:576	the drug substance nifedipine	548:576	the drug substance nifedipine (NIF)	548:582	KinetiSol® dispersing was used to thermally process hypromellose acetate succinate-based compositions containing the drug substance nifedipine (NIF) and a highly compressible grade of low-substituted hydroxypropyl cellulose (New Binder Disintegrants; NBD-grade).
25133663	1	49	theme	dosage	253:258	arg1	form					260:263	the finished dosage form	240:263	the finished dosage form	240:263	While the use of amorphous solid dispersions to improve aqueous solubility is well documented, little consideration has traditionally been given to the finished dosage form.
23701219	5	0	theme	AMC	673:675	arg1	pressure					754:761	pressure	754:761	pressure	754:761	The effects of AMC:EC ratio, the initial polymer concentration of the solution, temperature and pressure on the encapsulation process were investigated.
23701219	5	0	theme	AMC	673:675	arg1	temperature					738:748	temperature	738:748	temperature	738:748	The effects of AMC:EC ratio, the initial polymer concentration of the solution, temperature and pressure on the encapsulation process were investigated.
23701219	5	0	theme	AMC	673:675	arg1	concentration					707:719	the initial polymer concentration	687:719	the initial polymer concentration of the solution	687:735	The effects of AMC:EC ratio, the initial polymer concentration of the solution, temperature and pressure on the encapsulation process were investigated.
23701219	5	0	theme	AMC	673:675	arg1	ratio					680:684	AMC:EC ratio	673:684	AMC:EC ratio	673:684	The effects of AMC:EC ratio, the initial polymer concentration of the solution, temperature and pressure on the encapsulation process were investigated.
23701219	2	1	used	used	254:257	arg2	particles					267:275	host particles	262:275	host particles	262:275	AMC microparticles obtained previously by an SAS process were used as host particles and EC, a biodegradable polymer used for the controlled release of drugs, was chosen as the coating material.
23701219	2	1	used	used	254:257	arg2	microparticles					196:209	AMC microparticles	192:209	AMC microparticles obtained previously by an SAS process	192:247	AMC microparticles obtained previously by an SAS process were used as host particles and EC, a biodegradable polymer used for the controlled release of drugs, was chosen as the coating material.
23701219	1	2	theme	supercritical	134:146	arg1	SAS					169:171	SAS	169:171	SAS	169:171	Encapsulation of amoxicillin (AMC) with ethyl cellulose (EC) by a supercritical antisolvent process (SAS) was investigated.
23701219	1	2	theme	supercritical	134:146	arg1	process					160:166	a supercritical antisolvent process	132:166	a supercritical antisolvent process (SAS)	132:172	Encapsulation of amoxicillin (AMC) with ethyl cellulose (EC) by a supercritical antisolvent process (SAS) was investigated.
23701219	3	3	from	dichloromethane	472:486	arg1	solution					441:448	a solution	439:448	a solution of ethyl cellulose in dichloromethane (DCM)	439:492	In this work, a suspension of AMC microparticles in a solution of ethyl cellulose in dichloromethane (DCM) was sprayed through a nozzle into supercritical CO2.
23701219	9	4	dep	in	1191:1192	arg1	vitro					1194:1198	vitro	1194:1198	vitro	1194:1198	The in vitro release profiles of the resulting materials were evaluated in order to ascertain whether composites can be used as encapsulated systems for drug delivery systems.
23701219	3	5	from	solution	441:448	arg1	dichloromethane					472:486	dichloromethane	472:486	dichloromethane (DCM)	472:492	In this work, a suspension of AMC microparticles in a solution of ethyl cellulose in dichloromethane (DCM) was sprayed through a nozzle into supercritical CO2.
23701219	3	5	from	solution	441:448	arg1	DCM					489:491	DCM	489:491	DCM	489:491	In this work, a suspension of AMC microparticles in a solution of ethyl cellulose in dichloromethane (DCM) was sprayed through a nozzle into supercritical CO2.
23701219	2	6	theme	host	262:265	arg1	microparticles					196:209	AMC microparticles	192:209	AMC microparticles obtained previously by an SAS process	192:247	AMC microparticles obtained previously by an SAS process were used as host particles and EC, a biodegradable polymer used for the controlled release of drugs, was chosen as the coating material.
23701219	2	6	theme	host	262:265	arg1	particles					267:275	host particles	262:275	host particles	262:275	AMC microparticles obtained previously by an SAS process were used as host particles and EC, a biodegradable polymer used for the controlled release of drugs, was chosen as the coating material.
23701219	7	7	theme	AMC	1107:1109	arg1	ratio					1114:1118	the AMC:EC ratio	1103:1118	the AMC:EC ratio	1103:1118	In general, it was observed that the percentages of AMC present in the precipitates were higher on increasing the AMC:EC ratio.
23701219	7	8	located	observed	1012:1019	arg2	it					1005:1006	it	1005:1006	it	1005:1006	In general, it was observed that the percentages of AMC present in the precipitates were higher on increasing the AMC:EC ratio.
23701219	7	8	located	observed	1012:1019	arg1	general					996:1002	general	996:1002	general	996:1002	In general, it was observed that the percentages of AMC present in the precipitates were higher on increasing the AMC:EC ratio.
23701219	1	9	theme	ethyl	108:112	arg1	EC					125:126	EC	125:126	EC	125:126	Encapsulation of amoxicillin (AMC) with ethyl cellulose (EC) by a supercritical antisolvent process (SAS) was investigated.
23701219	1	9	theme	ethyl	108:112	arg1	cellulose					114:122	ethyl cellulose	108:122	ethyl cellulose (EC)	108:127	Encapsulation of amoxicillin (AMC) with ethyl cellulose (EC) by a supercritical antisolvent process (SAS) was investigated.
23701219	5	10	theme	solution	728:735	arg1	concentration					707:719	the initial polymer concentration	687:719	the initial polymer concentration of the solution	687:735	The effects of AMC:EC ratio, the initial polymer concentration of the solution, temperature and pressure on the encapsulation process were investigated.
23701219	5	10	theme	solution	728:735	arg1	ratio					680:684	AMC:EC ratio	673:684	AMC:EC ratio	673:684	The effects of AMC:EC ratio, the initial polymer concentration of the solution, temperature and pressure on the encapsulation process were investigated.
23701219	3	11	theme	cellulose	459:467	arg1	solution					441:448	a solution	439:448	a solution of ethyl cellulose in dichloromethane (DCM)	439:492	In this work, a suspension of AMC microparticles in a solution of ethyl cellulose in dichloromethane (DCM) was sprayed through a nozzle into supercritical CO2.
23701219	4	12	theme	photoelectron	589:601	arg1	XPS					617:619	XPS	617:619	XPS	617:619	Scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS) and HPLC analyses were carried out.
23701219	4	12	theme	photoelectron	589:601	arg1	spectroscopy					603:614	X-ray photoelectron spectroscopy	583:614	X-ray photoelectron spectroscopy (XPS)	583:620	Scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS) and HPLC analyses were carried out.
23701219	1	13	theme	antisolvent	148:158	arg1	SAS					169:171	SAS	169:171	SAS	169:171	Encapsulation of amoxicillin (AMC) with ethyl cellulose (EC) by a supercritical antisolvent process (SAS) was investigated.
23701219	1	13	theme	antisolvent	148:158	arg1	process					160:166	a supercritical antisolvent process	132:166	a supercritical antisolvent process (SAS)	132:172	Encapsulation of amoxicillin (AMC) with ethyl cellulose (EC) by a supercritical antisolvent process (SAS) was investigated.
23701219	1	14	theme	amoxicillin	85:95	arg1	Encapsulation					68:80	Encapsulation	68:80	Encapsulation of amoxicillin (AMC) with ethyl cellulose (EC) by a supercritical antisolvent process (SAS)	68:172	Encapsulation of amoxicillin (AMC) with ethyl cellulose (EC) by a supercritical antisolvent process (SAS) was investigated.
23701219	6	15	theme	drug	959:962	arg1	ratios					972:977	the lower drug:polymer ratios	949:977	the lower drug:polymer ratios	949:977	Although all the experiments led to powder precipitation, the AMC encapsulation was achieved in only half of the cases, particularly when the lower drug:polymer ratios were assayed.
23701219	5	16	theme	initial	691:697	arg1	concentration					707:719	the initial polymer concentration	687:719	the initial polymer concentration of the solution	687:735	The effects of AMC:EC ratio, the initial polymer concentration of the solution, temperature and pressure on the encapsulation process were investigated.
23701219	5	16	theme	initial	691:697	arg1	ratio					680:684	AMC:EC ratio	673:684	AMC:EC ratio	673:684	The effects of AMC:EC ratio, the initial polymer concentration of the solution, temperature and pressure on the encapsulation process were investigated.
23701219	6	17	theme	lower	953:957	arg1	ratios					972:977	the lower drug:polymer ratios	949:977	the lower drug:polymer ratios	949:977	Although all the experiments led to powder precipitation, the AMC encapsulation was achieved in only half of the cases, particularly when the lower drug:polymer ratios were assayed.
23701219	2	18	theme	drugs	344:348	arg1	release					333:339	the controlled release	318:339	the controlled release of drugs	318:348	AMC microparticles obtained previously by an SAS process were used as host particles and EC, a biodegradable polymer used for the controlled release of drugs, was chosen as the coating material.
23701219	0	19	theme	Polymer	0:6	arg1	encapsulation					8:20	Polymer encapsulation	0:20	Polymer encapsulation of amoxicillin	0:35	Polymer encapsulation of amoxicillin microparticles by SAS process.
23701219	6	20	theme	AMC	873:875	arg1	encapsulation					877:889	the AMC encapsulation	869:889	the AMC encapsulation	869:889	Although all the experiments led to powder precipitation, the AMC encapsulation was achieved in only half of the cases, particularly when the lower drug:polymer ratios were assayed.
23701219	5	21	theme	polymer	699:705	arg1	concentration					707:719	the initial polymer concentration	687:719	the initial polymer concentration of the solution	687:735	The effects of AMC:EC ratio, the initial polymer concentration of the solution, temperature and pressure on the encapsulation process were investigated.
23701219	5	21	theme	polymer	699:705	arg1	ratio					680:684	AMC:EC ratio	673:684	AMC:EC ratio	673:684	The effects of AMC:EC ratio, the initial polymer concentration of the solution, temperature and pressure on the encapsulation process were investigated.
23701219	9	22	used	used	1307:1310	arg2	composites					1289:1298	composites	1289:1298	composites	1289:1298	The in vitro release profiles of the resulting materials were evaluated in order to ascertain whether composites can be used as encapsulated systems for drug delivery systems.
23701219	9	22	used	used	1307:1310	arg2	systems					1328:1334	encapsulated systems	1315:1334	encapsulated systems for drug delivery systems	1315:1360	The in vitro release profiles of the resulting materials were evaluated in order to ascertain whether composites can be used as encapsulated systems for drug delivery systems.
23701219	4	23	theme	X-ray	583:587	arg1	XPS					617:619	XPS	617:619	XPS	617:619	Scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS) and HPLC analyses were carried out.
23701219	4	23	theme	X-ray	583:587	arg1	spectroscopy					603:614	X-ray photoelectron spectroscopy	583:614	X-ray photoelectron spectroscopy (XPS)	583:620	Scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS) and HPLC analyses were carried out.
23701219	0	24	theme	amoxicillin	25:35	arg1	encapsulation					8:20	Polymer encapsulation	0:20	Polymer encapsulation of amoxicillin	0:35	Polymer encapsulation of amoxicillin microparticles by SAS process.
23701219	2	25	theme	controlled	322:331	arg1	release					333:339	the controlled release	318:339	the controlled release of drugs	318:348	AMC microparticles obtained previously by an SAS process were used as host particles and EC, a biodegradable polymer used for the controlled release of drugs, was chosen as the coating material.
23701219	9	26	theme	encapsulated	1315:1326	arg1	composites					1289:1298	composites	1289:1298	composites	1289:1298	The in vitro release profiles of the resulting materials were evaluated in order to ascertain whether composites can be used as encapsulated systems for drug delivery systems.
23701219	9	26	theme	encapsulated	1315:1326	arg1	systems					1328:1334	encapsulated systems	1315:1334	encapsulated systems for drug delivery systems	1315:1360	The in vitro release profiles of the resulting materials were evaluated in order to ascertain whether composites can be used as encapsulated systems for drug delivery systems.
23701219	9	27	theme	resulting	1224:1232	arg1	materials					1234:1242	the resulting materials	1220:1242	the resulting materials	1220:1242	The in vitro release profiles of the resulting materials were evaluated in order to ascertain whether composites can be used as encapsulated systems for drug delivery systems.
23701219	2	28	theme	coating	369:375	arg1	material					377:384	the coating material	365:384	the coating material	365:384	AMC microparticles obtained previously by an SAS process were used as host particles and EC, a biodegradable polymer used for the controlled release of drugs, was chosen as the coating material.
23701219	2	28	theme	coating	369:375	arg1	EC					281:282	EC	281:282	EC	281:282	AMC microparticles obtained previously by an SAS process were used as host particles and EC, a biodegradable polymer used for the controlled release of drugs, was chosen as the coating material.
23701219	6	29	theme	polymer	964:970	arg1	ratios					972:977	the lower drug:polymer ratios	949:977	the lower drug:polymer ratios	949:977	Although all the experiments led to powder precipitation, the AMC encapsulation was achieved in only half of the cases, particularly when the lower drug:polymer ratios were assayed.
23701219	4	30	theme	electron	556:563	arg1	SEM					577:579	SEM	577:579	SEM	577:579	Scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS) and HPLC analyses were carried out.
23701219	4	30	theme	electron	556:563	arg1	microscopy					565:574	Scanning electron microscopy	547:574	Scanning electron microscopy (SEM)	547:580	Scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS) and HPLC analyses were carried out.
23701219	5	31	theme	EC	677:678	arg1	pressure					754:761	pressure	754:761	pressure	754:761	The effects of AMC:EC ratio, the initial polymer concentration of the solution, temperature and pressure on the encapsulation process were investigated.
23701219	5	31	theme	EC	677:678	arg1	temperature					738:748	temperature	738:748	temperature	738:748	The effects of AMC:EC ratio, the initial polymer concentration of the solution, temperature and pressure on the encapsulation process were investigated.
23701219	5	31	theme	EC	677:678	arg1	concentration					707:719	the initial polymer concentration	687:719	the initial polymer concentration of the solution	687:735	The effects of AMC:EC ratio, the initial polymer concentration of the solution, temperature and pressure on the encapsulation process were investigated.
23701219	5	31	theme	EC	677:678	arg1	ratio					680:684	AMC:EC ratio	673:684	AMC:EC ratio	673:684	The effects of AMC:EC ratio, the initial polymer concentration of the solution, temperature and pressure on the encapsulation process were investigated.
23701219	9	32	theme	release	1200:1206	arg1	profiles					1208:1215	The in vitro release profiles	1187:1215	The in vitro release profiles of the resulting materials	1187:1242	The in vitro release profiles of the resulting materials were evaluated in order to ascertain whether composites can be used as encapsulated systems for drug delivery systems.
23701219	7	33	theme	AMC	1045:1047	arg1	present					1049:1055	AMC present	1045:1055	AMC present	1045:1055	In general, it was observed that the percentages of AMC present in the precipitates were higher on increasing the AMC:EC ratio.
23701219	1	34	with	amoxicillin	85:95	arg1	EC					125:126	EC	125:126	EC	125:126	Encapsulation of amoxicillin (AMC) with ethyl cellulose (EC) by a supercritical antisolvent process (SAS) was investigated.
23701219	1	34	with	amoxicillin	85:95	arg1	cellulose					114:122	ethyl cellulose	108:122	ethyl cellulose (EC)	108:127	Encapsulation of amoxicillin (AMC) with ethyl cellulose (EC) by a supercritical antisolvent process (SAS) was investigated.
23701219	6	35	theme	powder	847:852	arg1	precipitation					854:866	powder precipitation	847:866	powder precipitation	847:866	Although all the experiments led to powder precipitation, the AMC encapsulation was achieved in only half of the cases, particularly when the lower drug:polymer ratios were assayed.
23701219	5	36	theme	ratio	680:684	arg1	effects					662:668	The effects	658:668	The effects of AMC:EC ratio, the initial polymer concentration of the solution, temperature and pressure on the encapsulation process	658:790	The effects of AMC:EC ratio, the initial polymer concentration of the solution, temperature and pressure on the encapsulation process were investigated.
23701219	7	37	from	percentages	1030:1040	arg1	precipitates					1064:1075	the precipitates	1060:1075	the precipitates	1060:1075	In general, it was observed that the percentages of AMC present in the precipitates were higher on increasing the AMC:EC ratio.
23701219	6	38	theme	cases	924:928	arg1	half					912:915	only half	907:915	only half of the cases	907:928	Although all the experiments led to powder precipitation, the AMC encapsulation was achieved in only half of the cases, particularly when the lower drug:polymer ratios were assayed.
23701219	9	39	theme	in	1191:1192	arg1	profiles					1208:1215	The in vitro release profiles	1187:1215	The in vitro release profiles of the resulting materials	1187:1242	The in vitro release profiles of the resulting materials were evaluated in order to ascertain whether composites can be used as encapsulated systems for drug delivery systems.
23701219	3	40	theme	microparticles	421:434	arg1	suspension					403:412	a suspension	401:412	a suspension of AMC microparticles in a solution of ethyl cellulose in dichloromethane (DCM)	401:492	In this work, a suspension of AMC microparticles in a solution of ethyl cellulose in dichloromethane (DCM) was sprayed through a nozzle into supercritical CO2.
23701219	0	41	theme	SAS	55:57	arg1	process					59:65	SAS process	55:65	SAS process	55:65	Polymer encapsulation of amoxicillin microparticles by SAS process.
23701219	2	42	theme	AMC	192:194	arg1	microparticles					196:209	AMC microparticles	192:209	AMC microparticles obtained previously by an SAS process	192:247	AMC microparticles obtained previously by an SAS process were used as host particles and EC, a biodegradable polymer used for the controlled release of drugs, was chosen as the coating material.
23701219	2	42	theme	AMC	192:194	arg1	particles					267:275	host particles	262:275	host particles	262:275	AMC microparticles obtained previously by an SAS process were used as host particles and EC, a biodegradable polymer used for the controlled release of drugs, was chosen as the coating material.
23701219	9	43	theme	drug	1340:1343	arg1	systems					1354:1360	drug delivery systems	1340:1360	drug delivery systems	1340:1360	The in vitro release profiles of the resulting materials were evaluated in order to ascertain whether composites can be used as encapsulated systems for drug delivery systems.
23701219	9	44	theme	delivery	1345:1352	arg1	systems					1354:1360	drug delivery systems	1340:1360	drug delivery systems	1340:1360	The in vitro release profiles of the resulting materials were evaluated in order to ascertain whether composites can be used as encapsulated systems for drug delivery systems.
23701219	7	45	theme	EC	1111:1112	arg1	ratio					1114:1118	the AMC:EC ratio	1103:1118	the AMC:EC ratio	1103:1118	In general, it was observed that the percentages of AMC present in the precipitates were higher on increasing the AMC:EC ratio.
23701219	4	46	theme	HPLC	626:629	arg1	analyses					631:638	HPLC analyses	626:638	HPLC analyses	626:638	Scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS) and HPLC analyses were carried out.
23701219	3	47	from	suspension	403:412	arg1	solution					441:448	a solution	439:448	a solution of ethyl cellulose in dichloromethane (DCM)	439:492	In this work, a suspension of AMC microparticles in a solution of ethyl cellulose in dichloromethane (DCM) was sprayed through a nozzle into supercritical CO2.
23701219	2	48	theme	biodegradable	287:299	arg1	polymer					301:307	a biodegradable polymer	285:307	a biodegradable polymer used for the controlled release of drugs	285:348	AMC microparticles obtained previously by an SAS process were used as host particles and EC, a biodegradable polymer used for the controlled release of drugs, was chosen as the coating material.
23701219	2	48	theme	biodegradable	287:299	arg1	EC					281:282	EC	281:282	EC	281:282	AMC microparticles obtained previously by an SAS process were used as host particles and EC, a biodegradable polymer used for the controlled release of drugs, was chosen as the coating material.
23701219	9	49	theme	materials	1234:1242	arg1	profiles					1208:1215	The in vitro release profiles	1187:1215	The in vitro release profiles of the resulting materials	1187:1242	The in vitro release profiles of the resulting materials were evaluated in order to ascertain whether composites can be used as encapsulated systems for drug delivery systems.
23701219	5	50	from	effects	662:668	arg1	process					784:790	the encapsulation process	766:790	the encapsulation process	766:790	The effects of AMC:EC ratio, the initial polymer concentration of the solution, temperature and pressure on the encapsulation process were investigated.
23701219	2	51	theme	SAS	237:239	arg1	process					241:247	an SAS process	234:247	an SAS process	234:247	AMC microparticles obtained previously by an SAS process were used as host particles and EC, a biodegradable polymer used for the controlled release of drugs, was chosen as the coating material.
23701219	3	52	theme	AMC	417:419	arg1	microparticles					421:434	AMC microparticles	417:434	AMC microparticles	417:434	In this work, a suspension of AMC microparticles in a solution of ethyl cellulose in dichloromethane (DCM) was sprayed through a nozzle into supercritical CO2.
23701219	3	53	theme	supercritical	528:540	arg1	CO2					542:544	supercritical CO2	528:544	supercritical CO2	528:544	In this work, a suspension of AMC microparticles in a solution of ethyl cellulose in dichloromethane (DCM) was sprayed through a nozzle into supercritical CO2.
23701219	5	54	theme	encapsulation	770:782	arg1	process					784:790	the encapsulation process	766:790	the encapsulation process	766:790	The effects of AMC:EC ratio, the initial polymer concentration of the solution, temperature and pressure on the encapsulation process were investigated.
23701219	4	55	theme	Scanning	547:554	arg1	SEM					577:579	SEM	577:579	SEM	577:579	Scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS) and HPLC analyses were carried out.
23701219	4	55	theme	Scanning	547:554	arg1	microscopy					565:574	Scanning electron microscopy	547:574	Scanning electron microscopy (SEM)	547:580	Scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS) and HPLC analyses were carried out.
23701219	7	56	theme	present	1049:1055	arg1	percentages					1030:1040	the percentages	1026:1040	the percentages of AMC present in the precipitates	1026:1075	In general, it was observed that the percentages of AMC present in the precipitates were higher on increasing the AMC:EC ratio.
23701219	7	56	theme	present	1049:1055	arg1	higher					1082:1087	higher	1082:1087	higher	1082:1087	In general, it was observed that the percentages of AMC present in the precipitates were higher on increasing the AMC:EC ratio.
23701219	3	57	theme	ethyl	453:457	arg1	cellulose					459:467	ethyl cellulose	453:467	ethyl cellulose in dichloromethane (DCM)	453:492	In this work, a suspension of AMC microparticles in a solution of ethyl cellulose in dichloromethane (DCM) was sprayed through a nozzle into supercritical CO2.
23701219	3	58	from	cellulose	459:467	arg1	dichloromethane					472:486	dichloromethane	472:486	dichloromethane (DCM)	472:492	In this work, a suspension of AMC microparticles in a solution of ethyl cellulose in dichloromethane (DCM) was sprayed through a nozzle into supercritical CO2.
23701219	3	58	from	cellulose	459:467	arg1	DCM					489:491	DCM	489:491	DCM	489:491	In this work, a suspension of AMC microparticles in a solution of ethyl cellulose in dichloromethane (DCM) was sprayed through a nozzle into supercritical CO2.
23701219	6	59	theme	only	907:910	arg1	half					912:915	only half	907:915	only half of the cases	907:928	Although all the experiments led to powder precipitation, the AMC encapsulation was achieved in only half of the cases, particularly when the lower drug:polymer ratios were assayed.
26677400	11	0	dep	provided	1455:1462	arg1	invasion					1530:1537	H7 invasion	1527:1537	H7 invasion into host cells	1527:1553	This study provided the first evidence of carbohydrate-dependent EPEC or EHEC O157:H7 invasion into host cells.
26677400	6	1	dep	increased	811:819	arg1	p < 0.05					862:869	p < 0.05	862:869	p < 0.05	862:869	EPEC or EHEC O157:H7 invasion into HT-29-OBN and HT-29-Gal-OBN cells increased compared with HT-29 and HT-29-Gal cells (p < 0.05 and p < 0.01).
26677400	6	1	dep	increased	811:819	arg1	p < 0.01					875:882	p < 0.01	875:882	p < 0.01	875:882	EPEC or EHEC O157:H7 invasion into HT-29-OBN and HT-29-Gal-OBN cells increased compared with HT-29 and HT-29-Gal cells (p < 0.05 and p < 0.01).
26677400	10	2	theme	O-glycosylation	1420:1434	arg1	status					1436:1441	their O-glycosylation status	1414:1441	their O-glycosylation status	1414:1441	CONCLUSION These data indicated that EPEC or EHEC O157:H7 invasion into HT-29 cells was related to their O-glycosylation status.
26677400	11	3	theme	H7	1527:1528	arg1	invasion					1530:1537	H7 invasion	1527:1537	H7 invasion into host cells	1527:1553	This study provided the first evidence of carbohydrate-dependent EPEC or EHEC O157:H7 invasion into host cells.
26677400	3	4	with	staining	356:363	arg1	PNA					370:372	PNA	370:372	PNA	370:372	RESULTS Lectin histochemical staining confirmed stronger staining with PNA, which labeled Galβ1, 3 GalNAc (core 1 structure) in HT-29-Gal-OBN and C2GnT2-sh2/HT-29 cells, compared with control cells.
26677400	4	5	theme	H7	516:517	arg1	invasion					519:526	H7 invasion	516:526	H7 invasion into HT-29 and its derived cells	516:559	EPEC or EHEC O157:H7 invasion into HT-29 and its derived cells was based on the intracellular presence of GFP-labeled bacteria.
26677400	3	6	theme	control	483:489	arg1	cells					491:495	control cells	483:495	control cells	483:495	RESULTS Lectin histochemical staining confirmed stronger staining with PNA, which labeled Galβ1, 3 GalNAc (core 1 structure) in HT-29-Gal-OBN and C2GnT2-sh2/HT-29 cells, compared with control cells.
26677400	8	7	theme	Bacterial	1049:1057	arg1	invasion					1059:1066	METHODS Bacterial invasion	1041:1066	METHODS Bacterial invasion into cultured cells	1041:1086	METHODS Bacterial invasion into cultured cells was determined by a gentamicin protection assay and a GFP-labeled bacteria invasion assay.
26677400	8	8	theme	protection	1119:1128	arg1	assay					1130:1134	a gentamicin protection assay	1106:1134	a gentamicin protection assay	1106:1134	METHODS Bacterial invasion into cultured cells was determined by a gentamicin protection assay and a GFP-labeled bacteria invasion assay.
26677400	4	9	theme	intracellular	578:590	arg1	presence					592:599	the intracellular presence	574:599	the intracellular presence of GFP-labeled bacteria	574:623	EPEC or EHEC O157:H7 invasion into HT-29 and its derived cells was based on the intracellular presence of GFP-labeled bacteria.
26677400	7	10	theme	EHEC	979:982	arg1	O157					984:987	EHEC O157	979:987	EHEC O157:H7 (p < 0.05)	979:1001	Core 2 O-glycan-deficient HT-29 cells underwent a significant increase in EPEC (p < 0.01) or EHEC O157:H7 (p < 0.05) invasion compared with control cells.
26677400	2	11	theme	O157	264:267	arg1	invasion					272:279	EPEC or EHEC O157:H7 invasion	251:279	EPEC or EHEC O157:H7 invasion into HT-29 cells	251:296	This study investigated whether and how O-glycans were involved in EPEC or EHEC O157:H7 invasion into HT-29 cells.
26677400	8	12	theme	GFP-labeled	1142:1152	arg1	assay					1172:1176	a GFP-labeled bacteria invasion assay	1140:1176	a GFP-labeled bacteria invasion assay	1140:1176	METHODS Bacterial invasion into cultured cells was determined by a gentamicin protection assay and a GFP-labeled bacteria invasion assay.
26677400	8	13	theme	invasion	1163:1170	arg1	assay					1172:1176	a GFP-labeled bacteria invasion assay	1140:1176	a GFP-labeled bacteria invasion assay	1140:1176	METHODS Bacterial invasion into cultured cells was determined by a gentamicin protection assay and a GFP-labeled bacteria invasion assay.
26677400	4	14	dep	O157	511:514	arg1	based					565:569	based	565:569	was based on the intracellular presence of GFP-labeled bacteria	561:623	EPEC or EHEC O157:H7 invasion into HT-29 and its derived cells was based on the intracellular presence of GFP-labeled bacteria.
26677400	6	15	theme	HT-29-Gal	845:853	arg1	cells					855:859	HT-29 and HT-29-Gal cells	835:859	cells	855:859	EPEC or EHEC O157:H7 invasion into HT-29-OBN and HT-29-Gal-OBN cells increased compared with HT-29 and HT-29-Gal cells (p < 0.05 and p < 0.01).
26677400	7	16	theme	O-glycan-deficient	893:910	arg1	cells					918:922	Core 2 O-glycan-deficient HT-29 cells	886:922	Core 2 O-glycan-deficient HT-29 cells	886:922	Core 2 O-glycan-deficient HT-29 cells underwent a significant increase in EPEC (p < 0.01) or EHEC O157:H7 (p < 0.05) invasion compared with control cells.
26677400	6	17	theme	HT-29	835:839	arg1	cells					855:859	HT-29 and HT-29-Gal cells	835:859	cells	855:859	EPEC or EHEC O157:H7 invasion into HT-29-OBN and HT-29-Gal-OBN cells increased compared with HT-29 and HT-29-Gal cells (p < 0.05 and p < 0.01).
26677400	7	18	theme	significant	936:946	arg1	increase					948:955	a significant increase	934:955	a significant increase in EPEC (p < 0.01) or EHEC O157:H7 (p < 0.05) invasion	934:1010	Core 2 O-glycan-deficient HT-29 cells underwent a significant increase in EPEC (p < 0.01) or EHEC O157:H7 (p < 0.05) invasion compared with control cells.
26677400	7	19	theme	Core	886:889	arg1	cells					918:922	Core 2 O-glycan-deficient HT-29 cells	886:922	Core 2 O-glycan-deficient HT-29 cells	886:922	Core 2 O-glycan-deficient HT-29 cells underwent a significant increase in EPEC (p < 0.01) or EHEC O157:H7 (p < 0.05) invasion compared with control cells.
26677400	1	20	theme	host	110:113	arg1	glycosylation					120:132	host cell glycosylation	110:132	host cell glycosylation	110:132	BACKGROUND How host cell glycosylation affects EPEC or EHEC O157:H7 invasion is unclear.
26677400	4	21	link	derived	547:553	arg1	cells					555:559	its derived cells	543:559	its derived cells	543:559	EPEC or EHEC O157:H7 invasion into HT-29 and its derived cells was based on the intracellular presence of GFP-labeled bacteria.
26677400	0	22	theme	O157	49:52	arg1	invasion					57:64	EPEC or EHEC O157:H7 invasion	36:64	EPEC or EHEC O157:H7 invasion into HT-29 epithelial cells	36:92	Core 2 mucin-type O-glycan inhibits EPEC or EHEC O157:H7 invasion into HT-29 epithelial cells.
26677400	4	23	theme	bacteria	616:623	arg1	presence					592:599	the intracellular presence	574:599	the intracellular presence of GFP-labeled bacteria	574:623	EPEC or EHEC O157:H7 invasion into HT-29 and its derived cells was based on the intracellular presence of GFP-labeled bacteria.
26677400	5	24	theme	EPEC	683:686	arg1	internalization					688:702	EPEC internalization	683:702	EPEC internalization	683:702	The differentiation of HT-29 cells led to a reduction in EPEC internalization compared with HT-29 cells (p < 0.01).
26677400	9	25	theme	O-glycans	1179:1187	arg1	biosynthesis					1189:1200	O-glycans biosynthesis	1179:1200	O-glycans biosynthesis	1179:1200	O-glycans biosynthesis was inhibited by benzyl-α-GalNAc, and core 2 O-glycan-deficient HT-29 cells were induced by C2GnT2 interference.
26677400	5	26	theme	cells	655:659	arg1	differentiation					630:644	The differentiation	626:644	The differentiation of HT-29 cells	626:659	The differentiation of HT-29 cells led to a reduction in EPEC internalization compared with HT-29 cells (p < 0.01).
26677400	0	27	theme	epithelial	77:86	arg1	cells					88:92	HT-29 epithelial cells	71:92	HT-29 epithelial cells	71:92	Core 2 mucin-type O-glycan inhibits EPEC or EHEC O157:H7 invasion into HT-29 epithelial cells.
26677400	0	28	theme	Core	0:3	arg1	O-glycan					18:25	Core 2 mucin-type O-glycan	0:25	Core 2 mucin-type O-glycan	0:25	Core 2 mucin-type O-glycan inhibits EPEC or EHEC O157:H7 invasion into HT-29 epithelial cells.
26677400	3	29	theme	histochemical	314:326	arg1	staining					328:335	Lectin histochemical staining	307:335	Lectin histochemical staining	307:335	RESULTS Lectin histochemical staining confirmed stronger staining with PNA, which labeled Galβ1, 3 GalNAc (core 1 structure) in HT-29-Gal-OBN and C2GnT2-sh2/HT-29 cells, compared with control cells.
26677400	0	30	theme	mucin-type	7:16	arg1	O-glycan					18:25	Core 2 mucin-type O-glycan	0:25	Core 2 mucin-type O-glycan	0:25	Core 2 mucin-type O-glycan inhibits EPEC or EHEC O157:H7 invasion into HT-29 epithelial cells.
26677400	9	31	theme	core	1240:1243	arg1	cells					1272:1276	core 2 O-glycan-deficient HT-29 cells	1240:1276	core 2 O-glycan-deficient HT-29 cells	1240:1276	O-glycans biosynthesis was inhibited by benzyl-α-GalNAc, and core 2 O-glycan-deficient HT-29 cells were induced by C2GnT2 interference.
26677400	0	32	dep	O157	49:52	arg1	H7					54:55	H7	54:55	EPEC or EHEC O157:H7 invasion into HT-29 epithelial cells	36:92	Core 2 mucin-type O-glycan inhibits EPEC or EHEC O157:H7 invasion into HT-29 epithelial cells.
26677400	4	33	theme	EPEC	498:501	arg1	O157					511:514	EPEC or EHEC O157	498:514	EPEC or EHEC O157:H7 invasion into HT-29 and its derived cells was based on the intracellular presence of GFP-labeled bacteria.	498:624	EPEC or EHEC O157:H7 invasion into HT-29 and its derived cells was based on the intracellular presence of GFP-labeled bacteria.
26677400	7	34	theme	control	1026:1032	arg1	cells					1034:1038	control cells	1026:1038	control cells	1026:1038	Core 2 O-glycan-deficient HT-29 cells underwent a significant increase in EPEC (p < 0.01) or EHEC O157:H7 (p < 0.05) invasion compared with control cells.
26677400	9	35	theme	O-glycan-deficient	1247:1264	arg1	cells					1272:1276	core 2 O-glycan-deficient HT-29 cells	1240:1276	core 2 O-glycan-deficient HT-29 cells	1240:1276	O-glycans biosynthesis was inhibited by benzyl-α-GalNAc, and core 2 O-glycan-deficient HT-29 cells were induced by C2GnT2 interference.
26677400	6	36	dep	O157	755:758	arg1	increased					811:819	increased	811:819	increased compared with HT-29 and HT-29-Gal cells (p < 0.05 and p < 0.01)	811:883	EPEC or EHEC O157:H7 invasion into HT-29-OBN and HT-29-Gal-OBN cells increased compared with HT-29 and HT-29-Gal cells (p < 0.05 and p < 0.01).
26677400	11	37	theme	EPEC	1509:1512	arg1	evidence					1474:1481	the first evidence	1464:1481	the first evidence of carbohydrate-dependent EPEC or EHEC O157	1464:1525	This study provided the first evidence of carbohydrate-dependent EPEC or EHEC O157:H7 invasion into host cells.
26677400	2	38	theme	HT-29	286:290	arg1	cells					292:296	HT-29 cells	286:296	HT-29 cells	286:296	This study investigated whether and how O-glycans were involved in EPEC or EHEC O157:H7 invasion into HT-29 cells.
26677400	5	39	theme	HT-29	718:722	arg1	p < 0.01					731:738	p < 0.01	731:738	p < 0.01	731:738	The differentiation of HT-29 cells led to a reduction in EPEC internalization compared with HT-29 cells (p < 0.01).
26677400	5	39	theme	HT-29	718:722	arg1	cells					724:728	HT-29 cells	718:728	HT-29 cells (p < 0.01)	718:739	The differentiation of HT-29 cells led to a reduction in EPEC internalization compared with HT-29 cells (p < 0.01).
26677400	6	40	theme	EHEC	750:753	arg1	O157					755:758	EPEC or EHEC O157	742:758	O157	755:758	EPEC or EHEC O157:H7 invasion into HT-29-OBN and HT-29-Gal-OBN cells increased compared with HT-29 and HT-29-Gal cells (p < 0.05 and p < 0.01).
26677400	3	41	dep	RESULTS	299:305	arg1	confirmed					337:345	confirmed	337:345	confirmed stronger staining with PNA, which labeled Galβ1, 3 GalNAc (core 1 structure) in HT-29-Gal-OBN and C2GnT2-sh2/HT-29 cells, compared with control cells	337:495	RESULTS Lectin histochemical staining confirmed stronger staining with PNA, which labeled Galβ1, 3 GalNAc (core 1 structure) in HT-29-Gal-OBN and C2GnT2-sh2/HT-29 cells, compared with control cells.
26677400	6	42	theme	EPEC	742:745	arg1	O157					755:758	EPEC or EHEC O157	742:758	O157	755:758	EPEC or EHEC O157:H7 invasion into HT-29-OBN and HT-29-Gal-OBN cells increased compared with HT-29 and HT-29-Gal cells (p < 0.05 and p < 0.01).
26677400	7	43	dep	EPEC	960:963	arg1	invasion					1003:1010	invasion	1003:1010	invasion	1003:1010	Core 2 O-glycan-deficient HT-29 cells underwent a significant increase in EPEC (p < 0.01) or EHEC O157:H7 (p < 0.05) invasion compared with control cells.
26677400	3	44	theme	C2GnT2-sh2/HT-29	445:460	arg1	cells					462:466	HT-29-Gal-OBN and C2GnT2-sh2/HT-29 cells	427:466	HT-29-Gal-OBN and C2GnT2-sh2/HT-29 cells	427:466	RESULTS Lectin histochemical staining confirmed stronger staining with PNA, which labeled Galβ1, 3 GalNAc (core 1 structure) in HT-29-Gal-OBN and C2GnT2-sh2/HT-29 cells, compared with control cells.
26677400	8	45	theme	gentamicin	1108:1117	arg1	assay					1130:1134	a gentamicin protection assay	1106:1134	a gentamicin protection assay	1106:1134	METHODS Bacterial invasion into cultured cells was determined by a gentamicin protection assay and a GFP-labeled bacteria invasion assay.
26677400	9	46	theme	C2GnT2	1294:1299	arg1	interference					1301:1312	C2GnT2 interference	1294:1312	C2GnT2 interference	1294:1312	O-glycans biosynthesis was inhibited by benzyl-α-GalNAc, and core 2 O-glycan-deficient HT-29 cells were induced by C2GnT2 interference.
26677400	11	47	theme	host	1544:1547	arg1	cells					1549:1553	host cells	1544:1553	host cells	1544:1553	This study provided the first evidence of carbohydrate-dependent EPEC or EHEC O157:H7 invasion into host cells.
26677400	11	48	theme	EHEC	1517:1520	arg1	O157					1522:1525	carbohydrate-dependent EPEC or EHEC O157	1486:1525	O157	1522:1525	This study provided the first evidence of carbohydrate-dependent EPEC or EHEC O157:H7 invasion into host cells.
26677400	3	49	theme	HT-29-Gal-OBN	427:439	arg1	cells					462:466	HT-29-Gal-OBN and C2GnT2-sh2/HT-29 cells	427:466	HT-29-Gal-OBN and C2GnT2-sh2/HT-29 cells	427:466	RESULTS Lectin histochemical staining confirmed stronger staining with PNA, which labeled Galβ1, 3 GalNAc (core 1 structure) in HT-29-Gal-OBN and C2GnT2-sh2/HT-29 cells, compared with control cells.
26677400	4	50	theme	EHEC	506:509	arg1	O157					511:514	EPEC or EHEC O157	498:514	EPEC or EHEC O157:H7 invasion into HT-29 and its derived cells was based on the intracellular presence of GFP-labeled bacteria.	498:624	EPEC or EHEC O157:H7 invasion into HT-29 and its derived cells was based on the intracellular presence of GFP-labeled bacteria.
26677400	2	51	theme	EPEC	251:254	arg1	O157					264:267	EPEC or EHEC O157	251:267	O157	264:267	This study investigated whether and how O-glycans were involved in EPEC or EHEC O157:H7 invasion into HT-29 cells.
26677400	6	52	theme	HT-29-Gal-OBN	791:803	arg1	cells					805:809	HT-29-OBN and HT-29-Gal-OBN cells	777:809	HT-29-OBN and HT-29-Gal-OBN cells	777:809	EPEC or EHEC O157:H7 invasion into HT-29-OBN and HT-29-Gal-OBN cells increased compared with HT-29 and HT-29-Gal cells (p < 0.05 and p < 0.01).
26677400	8	53	theme	METHODS	1041:1047	arg1	invasion					1059:1066	METHODS Bacterial invasion	1041:1066	METHODS Bacterial invasion into cultured cells	1041:1086	METHODS Bacterial invasion into cultured cells was determined by a gentamicin protection assay and a GFP-labeled bacteria invasion assay.
26677400	6	54	theme	HT-29-OBN	777:785	arg1	cells					805:809	HT-29-OBN and HT-29-Gal-OBN cells	777:809	HT-29-OBN and HT-29-Gal-OBN cells	777:809	EPEC or EHEC O157:H7 invasion into HT-29-OBN and HT-29-Gal-OBN cells increased compared with HT-29 and HT-29-Gal cells (p < 0.05 and p < 0.01).
26677400	2	55	dep	whether	208:214	arg1	involved					239:246	involved	239:246	were involved in EPEC or EHEC O157:H7 invasion into HT-29 cells	234:296	This study investigated whether and how O-glycans were involved in EPEC or EHEC O157:H7 invasion into HT-29 cells.
26677400	11	56	theme	first	1468:1472	arg1	evidence					1474:1481	the first evidence	1464:1481	the first evidence of carbohydrate-dependent EPEC or EHEC O157	1464:1525	This study provided the first evidence of carbohydrate-dependent EPEC or EHEC O157:H7 invasion into host cells.
26677400	2	57	theme	EHEC	259:262	arg1	O157					264:267	EPEC or EHEC O157	251:267	O157	264:267	This study investigated whether and how O-glycans were involved in EPEC or EHEC O157:H7 invasion into HT-29 cells.
26677400	1	58	theme	H7	160:161	arg1	invasion					163:170	H7 invasion	160:170	H7 invasion	160:170	BACKGROUND How host cell glycosylation affects EPEC or EHEC O157:H7 invasion is unclear.
26677400	8	59	theme	cultured	1073:1080	arg1	cells					1082:1086	cultured cells	1073:1086	cultured cells	1073:1086	METHODS Bacterial invasion into cultured cells was determined by a gentamicin protection assay and a GFP-labeled bacteria invasion assay.
26677400	8	60	theme	bacteria	1154:1161	arg1	assay					1172:1176	a GFP-labeled bacteria invasion assay	1140:1176	a GFP-labeled bacteria invasion assay	1140:1176	METHODS Bacterial invasion into cultured cells was determined by a gentamicin protection assay and a GFP-labeled bacteria invasion assay.
26677400	7	61	dep	O157	984:987	arg1	H7					989:990	H7 (p < 0.05)	989:1001	EHEC O157:H7 (p < 0.05)	979:1001	Core 2 O-glycan-deficient HT-29 cells underwent a significant increase in EPEC (p < 0.01) or EHEC O157:H7 (p < 0.05) invasion compared with control cells.
26677400	5	62	theme	HT-29	649:653	arg1	cells					655:659	HT-29 cells	649:659	HT-29 cells	649:659	The differentiation of HT-29 cells led to a reduction in EPEC internalization compared with HT-29 cells (p < 0.01).
26677400	7	63	theme	HT-29	912:916	arg1	cells					918:922	Core 2 O-glycan-deficient HT-29 cells	886:922	Core 2 O-glycan-deficient HT-29 cells	886:922	Core 2 O-glycan-deficient HT-29 cells underwent a significant increase in EPEC (p < 0.01) or EHEC O157:H7 (p < 0.05) invasion compared with control cells.
26677400	2	64	dep	O157	264:267	arg1	H7					269:270	H7	269:270	EPEC or EHEC O157:H7 invasion into HT-29 cells	251:296	This study investigated whether and how O-glycans were involved in EPEC or EHEC O157:H7 invasion into HT-29 cells.
26677400	5	65	from	reduction	670:678	arg1	internalization					688:702	EPEC internalization	683:702	EPEC internalization	683:702	The differentiation of HT-29 cells led to a reduction in EPEC internalization compared with HT-29 cells (p < 0.01).
26677400	4	66	theme	derived	547:553	arg1	cells					555:559	its derived cells	543:559	its derived cells	543:559	EPEC or EHEC O157:H7 invasion into HT-29 and its derived cells was based on the intracellular presence of GFP-labeled bacteria.
26677400	1	67	theme	cell	115:118	arg1	glycosylation					120:132	host cell glycosylation	110:132	host cell glycosylation	110:132	BACKGROUND How host cell glycosylation affects EPEC or EHEC O157:H7 invasion is unclear.
26677400	10	68	theme	EPEC	1352:1355	arg1	O157					1365:1368	EPEC or EHEC O157	1352:1368	O157	1365:1368	CONCLUSION These data indicated that EPEC or EHEC O157:H7 invasion into HT-29 cells was related to their O-glycosylation status.
26677400	4	69	theme	GFP-labeled	604:614	arg1	bacteria					616:623	GFP-labeled bacteria	604:623	GFP-labeled bacteria	604:623	EPEC or EHEC O157:H7 invasion into HT-29 and its derived cells was based on the intracellular presence of GFP-labeled bacteria.
26677400	0	70	theme	HT-29	71:75	arg1	cells					88:92	HT-29 epithelial cells	71:92	HT-29 epithelial cells	71:92	Core 2 mucin-type O-glycan inhibits EPEC or EHEC O157:H7 invasion into HT-29 epithelial cells.
26677400	3	71	theme	Lectin	307:312	arg1	staining					328:335	Lectin histochemical staining	307:335	Lectin histochemical staining	307:335	RESULTS Lectin histochemical staining confirmed stronger staining with PNA, which labeled Galβ1, 3 GalNAc (core 1 structure) in HT-29-Gal-OBN and C2GnT2-sh2/HT-29 cells, compared with control cells.
26677400	1	72	dep	BACKGROUND	95:104	arg1	affects					134:140	affects	134:140	affects EPEC or EHEC O157	134:158	BACKGROUND How host cell glycosylation affects EPEC or EHEC O157:H7 invasion is unclear.
26677400	1	72	dep	BACKGROUND	95:104	arg1	unclear					175:181	unclear	175:181	unclear	175:181	BACKGROUND How host cell glycosylation affects EPEC or EHEC O157:H7 invasion is unclear.
26677400	10	73	dep	O157	1365:1368	arg1	H7					1370:1371	H7	1370:1371	EPEC or EHEC O157:H7 invasion into HT-29 cells	1352:1397	CONCLUSION These data indicated that EPEC or EHEC O157:H7 invasion into HT-29 cells was related to their O-glycosylation status.
26677400	3	74	theme	stronger	347:354	arg1	staining					356:363	stronger staining	347:363	stronger staining	347:363	RESULTS Lectin histochemical staining confirmed stronger staining with PNA, which labeled Galβ1, 3 GalNAc (core 1 structure) in HT-29-Gal-OBN and C2GnT2-sh2/HT-29 cells, compared with control cells.
26677400	10	75	theme	EHEC	1360:1363	arg1	O157					1365:1368	EPEC or EHEC O157	1352:1368	O157	1365:1368	CONCLUSION These data indicated that EPEC or EHEC O157:H7 invasion into HT-29 cells was related to their O-glycosylation status.
26677400	1	76	dep	EPEC	142:145	arg1	O157					155:158	O157	155:158	O157	155:158	BACKGROUND How host cell glycosylation affects EPEC or EHEC O157:H7 invasion is unclear.
26677400	10	77	dep	H7	1370:1371	arg1	invasion					1373:1380	invasion	1373:1380	EPEC or EHEC O157:H7 invasion into HT-29 cells	1352:1397	CONCLUSION These data indicated that EPEC or EHEC O157:H7 invasion into HT-29 cells was related to their O-glycosylation status.
26677400	2	78	dep	investigated	195:206	arg1	whether					208:214	whether	208:214	whether	208:214	This study investigated whether and how O-glycans were involved in EPEC or EHEC O157:H7 invasion into HT-29 cells.
26677400	10	79	theme	HT-29	1387:1391	arg1	cells					1393:1397	HT-29 cells	1387:1397	HT-29 cells	1387:1397	CONCLUSION These data indicated that EPEC or EHEC O157:H7 invasion into HT-29 cells was related to their O-glycosylation status.
26677400	3	80	theme	core	406:409	arg1	Galβ1					389:393	Galβ1	389:393	Galβ1	389:393	RESULTS Lectin histochemical staining confirmed stronger staining with PNA, which labeled Galβ1, 3 GalNAc (core 1 structure) in HT-29-Gal-OBN and C2GnT2-sh2/HT-29 cells, compared with control cells.
26677400	3	80	theme	core	406:409	arg1	structure					413:421	core 1 structure	406:421	core 1 structure	406:421	RESULTS Lectin histochemical staining confirmed stronger staining with PNA, which labeled Galβ1, 3 GalNAc (core 1 structure) in HT-29-Gal-OBN and C2GnT2-sh2/HT-29 cells, compared with control cells.
26677400	0	81	theme	EPEC	36:39	arg1	O157					49:52	EPEC or EHEC O157	36:52	O157	49:52	Core 2 mucin-type O-glycan inhibits EPEC or EHEC O157:H7 invasion into HT-29 epithelial cells.
26677400	6	82	theme	H7	760:761	arg1	invasion					763:770	H7 invasion	760:770	H7 invasion into HT-29-OBN and HT-29-Gal-OBN cells	760:809	EPEC or EHEC O157:H7 invasion into HT-29-OBN and HT-29-Gal-OBN cells increased compared with HT-29 and HT-29-Gal cells (p < 0.05 and p < 0.01).
26677400	11	83	theme	carbohydrate-dependent	1486:1507	arg1	EPEC					1509:1512	carbohydrate-dependent EPEC or EHEC O157	1486:1525	EPEC	1509:1512	This study provided the first evidence of carbohydrate-dependent EPEC or EHEC O157:H7 invasion into host cells.
26677400	0	84	theme	EHEC	44:47	arg1	O157					49:52	EPEC or EHEC O157	36:52	O157	49:52	Core 2 mucin-type O-glycan inhibits EPEC or EHEC O157:H7 invasion into HT-29 epithelial cells.
26677400	9	85	theme	HT-29	1266:1270	arg1	cells					1272:1276	core 2 O-glycan-deficient HT-29 cells	1240:1276	core 2 O-glycan-deficient HT-29 cells	1240:1276	O-glycans biosynthesis was inhibited by benzyl-α-GalNAc, and core 2 O-glycan-deficient HT-29 cells were induced by C2GnT2 interference.
26677400	10	86	dep	CONCLUSION	1315:1324	arg1	indicated					1337:1345	indicated	1337:1345	indicated that EPEC or EHEC O157:H7 invasion into HT-29 cells was related to their O-glycosylation status	1337:1441	CONCLUSION These data indicated that EPEC or EHEC O157:H7 invasion into HT-29 cells was related to their O-glycosylation status.
26677400	11	87	theme	O157	1522:1525	arg1	evidence					1474:1481	the first evidence	1464:1481	the first evidence of carbohydrate-dependent EPEC or EHEC O157	1464:1525	This study provided the first evidence of carbohydrate-dependent EPEC or EHEC O157:H7 invasion into host cells.
26677400	7	88	from	increase	948:955	arg1	O157					984:987	EHEC O157	979:987	EHEC O157:H7 (p < 0.05)	979:1001	Core 2 O-glycan-deficient HT-29 cells underwent a significant increase in EPEC (p < 0.01) or EHEC O157:H7 (p < 0.05) invasion compared with control cells.
26677400	7	88	from	increase	948:955	arg1	p < 0.01					966:973	p < 0.01	966:973	p < 0.01	966:973	Core 2 O-glycan-deficient HT-29 cells underwent a significant increase in EPEC (p < 0.01) or EHEC O157:H7 (p < 0.05) invasion compared with control cells.
26677400	7	88	from	increase	948:955	arg1	EPEC					960:963	EPEC	960:963	EPEC (p < 0.01)	960:974	Core 2 O-glycan-deficient HT-29 cells underwent a significant increase in EPEC (p < 0.01) or EHEC O157:H7 (p < 0.05) invasion compared with control cells.
26677400	3	89	from	Galβ1	389:393	arg1	cells					462:466	HT-29-Gal-OBN and C2GnT2-sh2/HT-29 cells	427:466	HT-29-Gal-OBN and C2GnT2-sh2/HT-29 cells	427:466	RESULTS Lectin histochemical staining confirmed stronger staining with PNA, which labeled Galβ1, 3 GalNAc (core 1 structure) in HT-29-Gal-OBN and C2GnT2-sh2/HT-29 cells, compared with control cells.
25933528	6	0	theme	pathogens	1022:1030	arg1	inhibition					992:1001	the growth inhibition	981:1001	the growth inhibition of common clinical pathogens, such as Staphylococcus aureus and Escherichia coli	981:1082	Our results showed that the berberine-loaded beads and nanoparticles/beads complex could effectively inhibit the growth inhibition of common clinical pathogens, such as Staphylococcus aureus and Escherichia coli, and have the advantage of continually releasing berberine to inhibit the growth of the bacteria over 24 h.
25933528	5	1	theme	berberine	791:799	arg1	release					801:807	the berberine release	787:807	the berberine release in simulated gastric fluid	787:834	The nanoparticles/beads complex served as a drug carrier to delay the berberine release in simulated gastric fluid, with an estimated lag time of 2 h.
25933528	4	2	theme	chitosan	705:712	arg1	beads					714:718	the fucoidan-shelled chitosan beads	684:718	the fucoidan-shelled chitosan beads	684:718	Furthermore, a nanoparticles/beads complex system was developed by incorporation of berberine-loaded chitosan/fucoidan nanoparticles in the fucoidan-shelled chitosan beads.
25933528	6	3	theme	clinical	1013:1020	arg1	aureus					1056:1061	Staphylococcus aureus	1041:1061	Staphylococcus aureus	1041:1061	Our results showed that the berberine-loaded beads and nanoparticles/beads complex could effectively inhibit the growth inhibition of common clinical pathogens, such as Staphylococcus aureus and Escherichia coli, and have the advantage of continually releasing berberine to inhibit the growth of the bacteria over 24 h.
25933528	6	3	theme	clinical	1013:1020	arg1	pathogens					1022:1030	common clinical pathogens	1006:1030	common clinical pathogens	1006:1030	Our results showed that the berberine-loaded beads and nanoparticles/beads complex could effectively inhibit the growth inhibition of common clinical pathogens, such as Staphylococcus aureus and Escherichia coli, and have the advantage of continually releasing berberine to inhibit the growth of the bacteria over 24 h.
25933528	6	3	theme	clinical	1013:1020	arg1	coli					1079:1082	Escherichia coli	1067:1082	Escherichia coli	1067:1082	Our results showed that the berberine-loaded beads and nanoparticles/beads complex could effectively inhibit the growth inhibition of common clinical pathogens, such as Staphylococcus aureus and Escherichia coli, and have the advantage of continually releasing berberine to inhibit the growth of the bacteria over 24 h.
25933528	5	4	from	release	801:807	arg1	fluid					830:834	simulated gastric fluid	812:834	simulated gastric fluid	812:834	The nanoparticles/beads complex served as a drug carrier to delay the berberine release in simulated gastric fluid, with an estimated lag time of 2 h.
25933528	3	5	theme	intestinal	521:530	arg1	pH					539:540	pH 7.4	539:544	pH 7.4	539:544	The drug release of the berberine-loaded beads was faster in simulated gastric fluid (pH 1.2) than those in simulated intestinal fluid (pH 7.4).
25933528	3	5	theme	intestinal	521:530	arg1	fluid					532:536	simulated intestinal fluid	511:536	simulated intestinal fluid (pH 7.4)	511:545	The drug release of the berberine-loaded beads was faster in simulated gastric fluid (pH 1.2) than those in simulated intestinal fluid (pH 7.4).
25933528	4	6	theme	chitosan/fucoidan	649:665	arg1	nanoparticles					667:679	berberine-loaded chitosan/fucoidan nanoparticles	632:679	berberine-loaded chitosan/fucoidan nanoparticles	632:679	Furthermore, a nanoparticles/beads complex system was developed by incorporation of berberine-loaded chitosan/fucoidan nanoparticles in the fucoidan-shelled chitosan beads.
25933528	6	7	theme	common	1006:1011	arg1	aureus					1056:1061	Staphylococcus aureus	1041:1061	Staphylococcus aureus	1041:1061	Our results showed that the berberine-loaded beads and nanoparticles/beads complex could effectively inhibit the growth inhibition of common clinical pathogens, such as Staphylococcus aureus and Escherichia coli, and have the advantage of continually releasing berberine to inhibit the growth of the bacteria over 24 h.
25933528	6	7	theme	common	1006:1011	arg1	pathogens					1022:1030	common clinical pathogens	1006:1030	common clinical pathogens	1006:1030	Our results showed that the berberine-loaded beads and nanoparticles/beads complex could effectively inhibit the growth inhibition of common clinical pathogens, such as Staphylococcus aureus and Escherichia coli, and have the advantage of continually releasing berberine to inhibit the growth of the bacteria over 24 h.
25933528	6	7	theme	common	1006:1011	arg1	coli					1079:1082	Escherichia coli	1067:1082	Escherichia coli	1067:1082	Our results showed that the berberine-loaded beads and nanoparticles/beads complex could effectively inhibit the growth inhibition of common clinical pathogens, such as Staphylococcus aureus and Escherichia coli, and have the advantage of continually releasing berberine to inhibit the growth of the bacteria over 24 h.
25933528	5	8	theme	estimated	845:853	arg1	time					859:862	an estimated lag time	842:862	an estimated lag time of 2 h	842:869	The nanoparticles/beads complex served as a drug carrier to delay the berberine release in simulated gastric fluid, with an estimated lag time of 2 h.
25933528	2	9	theme	combined	379:386	arg1	agent					396:400	the genipin/fucoidan combined gelling agent	358:400	the genipin/fucoidan combined gelling agent	358:400	The cross-linking level and swelling property of the beads were affected by the pH value and the composition of the genipin/fucoidan combined gelling agent.
25933528	2	10	theme	agent	396:400	arg1	value					329:333	the pH value	322:333	the pH value	322:333	The cross-linking level and swelling property of the beads were affected by the pH value and the composition of the genipin/fucoidan combined gelling agent.
25933528	2	10	theme	agent	396:400	arg1	composition					343:353	the composition	339:353	the composition of the genipin/fucoidan combined gelling agent	339:400	The cross-linking level and swelling property of the beads were affected by the pH value and the composition of the genipin/fucoidan combined gelling agent.
25933528	5	11	theme	drug	765:768	arg1	carrier					770:776	a drug carrier	763:776	a drug carrier to delay the berberine release in simulated gastric fluid, with an estimated lag time of 2 h	763:869	The nanoparticles/beads complex served as a drug carrier to delay the berberine release in simulated gastric fluid, with an estimated lag time of 2 h.
25933528	5	11	theme	drug	765:768	arg1	complex					745:751	The nanoparticles/beads complex	721:751	The nanoparticles/beads complex	721:751	The nanoparticles/beads complex served as a drug carrier to delay the berberine release in simulated gastric fluid, with an estimated lag time of 2 h.
25933528	0	12	theme	chitosan	56:63	arg1	beads					65:69	fucoidan-shelled and genipin-crosslinked chitosan beads	15:69	fucoidan-shelled and genipin-crosslinked chitosan beads	15:69	Preparation of fucoidan-shelled and genipin-crosslinked chitosan beads for antibacterial application.
25933528	2	13	theme	swelling	274:281	arg1	property					283:290	swelling property	274:290	swelling property	274:290	The cross-linking level and swelling property of the beads were affected by the pH value and the composition of the genipin/fucoidan combined gelling agent.
25933528	1	14	theme	oral	184:187	arg1	delivery					189:196	oral delivery	184:196	oral delivery of berberine	184:209	In this study, a fucoidan-shelled chitosan bead was developed with the purpose of oral delivery of berberine to inhibit the growth of bacteria.
25933528	5	15	theme	gastric	822:828	arg1	fluid					830:834	simulated gastric fluid	812:834	simulated gastric fluid	812:834	The nanoparticles/beads complex served as a drug carrier to delay the berberine release in simulated gastric fluid, with an estimated lag time of 2 h.
25933528	4	16	theme	berberine-loaded	632:647	arg1	nanoparticles					667:679	berberine-loaded chitosan/fucoidan nanoparticles	632:679	berberine-loaded chitosan/fucoidan nanoparticles	632:679	Furthermore, a nanoparticles/beads complex system was developed by incorporation of berberine-loaded chitosan/fucoidan nanoparticles in the fucoidan-shelled chitosan beads.
25933528	6	17	theme	bacteria	1172:1179	arg1	growth					1158:1163	the growth	1154:1163	the growth of the bacteria	1154:1179	Our results showed that the berberine-loaded beads and nanoparticles/beads complex could effectively inhibit the growth inhibition of common clinical pathogens, such as Staphylococcus aureus and Escherichia coli, and have the advantage of continually releasing berberine to inhibit the growth of the bacteria over 24 h.
25933528	1	18	theme	delivery	189:196	arg1	purpose					173:179	the purpose	169:179	the purpose of oral delivery of berberine to inhibit the growth of bacteria	169:243	In this study, a fucoidan-shelled chitosan bead was developed with the purpose of oral delivery of berberine to inhibit the growth of bacteria.
25933528	6	19	contain	have	1089:1092	arg2	advantage					1098:1106	the advantage	1094:1106	the advantage of continually releasing berberine to inhibit the growth of the bacteria over 24 h	1094:1189	Our results showed that the berberine-loaded beads and nanoparticles/beads complex could effectively inhibit the growth inhibition of common clinical pathogens, such as Staphylococcus aureus and Escherichia coli, and have the advantage of continually releasing berberine to inhibit the growth of the bacteria over 24 h.
25933528	6	19	contain	have	1089:1092	arg1	beads					917:921	the berberine-loaded beads	896:921	the berberine-loaded beads	896:921	Our results showed that the berberine-loaded beads and nanoparticles/beads complex could effectively inhibit the growth inhibition of common clinical pathogens, such as Staphylococcus aureus and Escherichia coli, and have the advantage of continually releasing berberine to inhibit the growth of the bacteria over 24 h.
25933528	2	20	dep	level	264:268	arg1	The					246:248	The	246:248	The	246:248	The cross-linking level and swelling property of the beads were affected by the pH value and the composition of the genipin/fucoidan combined gelling agent.
25933528	4	21	theme	nanoparticles/beads	563:581	arg1	system					591:596	a nanoparticles/beads complex system	561:596	a nanoparticles/beads complex system	561:596	Furthermore, a nanoparticles/beads complex system was developed by incorporation of berberine-loaded chitosan/fucoidan nanoparticles in the fucoidan-shelled chitosan beads.
25933528	6	22	theme	growth	985:990	arg1	inhibition					992:1001	the growth inhibition	981:1001	the growth inhibition of common clinical pathogens, such as Staphylococcus aureus and Escherichia coli	981:1082	Our results showed that the berberine-loaded beads and nanoparticles/beads complex could effectively inhibit the growth inhibition of common clinical pathogens, such as Staphylococcus aureus and Escherichia coli, and have the advantage of continually releasing berberine to inhibit the growth of the bacteria over 24 h.
25933528	0	23	link	genipin-crosslinked	36:54	arg1	beads					65:69	fucoidan-shelled and genipin-crosslinked chitosan beads	15:69	fucoidan-shelled and genipin-crosslinked chitosan beads	15:69	Preparation of fucoidan-shelled and genipin-crosslinked chitosan beads for antibacterial application.
25933528	4	24	from	incorporation	615:627	arg1	beads					714:718	the fucoidan-shelled chitosan beads	684:718	the fucoidan-shelled chitosan beads	684:718	Furthermore, a nanoparticles/beads complex system was developed by incorporation of berberine-loaded chitosan/fucoidan nanoparticles in the fucoidan-shelled chitosan beads.
25933528	1	25	theme	berberine	201:209	arg1	delivery					189:196	oral delivery	184:196	oral delivery of berberine	184:209	In this study, a fucoidan-shelled chitosan bead was developed with the purpose of oral delivery of berberine to inhibit the growth of bacteria.
25933528	0	26	theme	fucoidan-shelled	15:30	arg1	beads					65:69	fucoidan-shelled and genipin-crosslinked chitosan beads	15:69	fucoidan-shelled and genipin-crosslinked chitosan beads	15:69	Preparation of fucoidan-shelled and genipin-crosslinked chitosan beads for antibacterial application.
25933528	2	27	theme	beads	299:303	arg1	level					264:268	cross-linking level	250:268	cross-linking level	250:268	The cross-linking level and swelling property of the beads were affected by the pH value and the composition of the genipin/fucoidan combined gelling agent.
25933528	2	27	theme	beads	299:303	arg1	property					283:290	swelling property	274:290	swelling property	274:290	The cross-linking level and swelling property of the beads were affected by the pH value and the composition of the genipin/fucoidan combined gelling agent.
25933528	3	28	theme	simulated	464:472	arg1	pH					489:490	pH 1.2	489:494	pH 1.2	489:494	The drug release of the berberine-loaded beads was faster in simulated gastric fluid (pH 1.2) than those in simulated intestinal fluid (pH 7.4).
25933528	3	28	theme	simulated	464:472	arg1	fluid					482:486	simulated gastric fluid	464:486	simulated gastric fluid (pH 1.2)	464:495	The drug release of the berberine-loaded beads was faster in simulated gastric fluid (pH 1.2) than those in simulated intestinal fluid (pH 7.4).
25933528	3	29	theme	drug	407:410	arg1	faster					454:459	faster	454:459	faster	454:459	The drug release of the berberine-loaded beads was faster in simulated gastric fluid (pH 1.2) than those in simulated intestinal fluid (pH 7.4).
25933528	3	29	theme	drug	407:410	arg1	release					412:418	The drug release	403:418	The drug release of the berberine-loaded beads	403:448	The drug release of the berberine-loaded beads was faster in simulated gastric fluid (pH 1.2) than those in simulated intestinal fluid (pH 7.4).
25933528	5	30	theme	nanoparticles/beads	725:743	arg1	carrier					770:776	a drug carrier	763:776	a drug carrier to delay the berberine release in simulated gastric fluid, with an estimated lag time of 2 h	763:869	The nanoparticles/beads complex served as a drug carrier to delay the berberine release in simulated gastric fluid, with an estimated lag time of 2 h.
25933528	5	30	theme	nanoparticles/beads	725:743	arg1	complex					745:751	The nanoparticles/beads complex	721:751	The nanoparticles/beads complex	721:751	The nanoparticles/beads complex served as a drug carrier to delay the berberine release in simulated gastric fluid, with an estimated lag time of 2 h.
25933528	2	31	theme	genipin/fucoidan	362:377	arg1	agent					396:400	the genipin/fucoidan combined gelling agent	358:400	the genipin/fucoidan combined gelling agent	358:400	The cross-linking level and swelling property of the beads were affected by the pH value and the composition of the genipin/fucoidan combined gelling agent.
25933528	5	32	theme	lag	855:857	arg1	time					859:862	an estimated lag time	842:862	an estimated lag time of 2 h	842:869	The nanoparticles/beads complex served as a drug carrier to delay the berberine release in simulated gastric fluid, with an estimated lag time of 2 h.
25933528	0	33	theme	genipin-crosslinked	36:54	arg1	beads					65:69	fucoidan-shelled and genipin-crosslinked chitosan beads	15:69	fucoidan-shelled and genipin-crosslinked chitosan beads	15:69	Preparation of fucoidan-shelled and genipin-crosslinked chitosan beads for antibacterial application.
25933528	5	34	theme	h	869:869	arg1	time					859:862	an estimated lag time	842:862	an estimated lag time of 2 h	842:869	The nanoparticles/beads complex served as a drug carrier to delay the berberine release in simulated gastric fluid, with an estimated lag time of 2 h.
25933528	5	35	theme	simulated	812:820	arg1	fluid					830:834	simulated gastric fluid	812:834	simulated gastric fluid	812:834	The nanoparticles/beads complex served as a drug carrier to delay the berberine release in simulated gastric fluid, with an estimated lag time of 2 h.
25933528	3	36	theme	berberine-loaded	427:442	arg1	beads					444:448	the berberine-loaded beads	423:448	the berberine-loaded beads	423:448	The drug release of the berberine-loaded beads was faster in simulated gastric fluid (pH 1.2) than those in simulated intestinal fluid (pH 7.4).
25933528	6	37	theme	releasing	1123:1131	arg1	berberine					1133:1141	continually releasing berberine	1111:1141	continually releasing berberine	1111:1141	Our results showed that the berberine-loaded beads and nanoparticles/beads complex could effectively inhibit the growth inhibition of common clinical pathogens, such as Staphylococcus aureus and Escherichia coli, and have the advantage of continually releasing berberine to inhibit the growth of the bacteria over 24 h.
25933528	1	38	theme	fucoidan-shelled	119:134	arg1	bead					145:148	a fucoidan-shelled chitosan bead	117:148	a fucoidan-shelled chitosan bead	117:148	In this study, a fucoidan-shelled chitosan bead was developed with the purpose of oral delivery of berberine to inhibit the growth of bacteria.
25933528	0	39	theme	beads	65:69	arg1	Preparation					0:10	Preparation	0:10	Preparation of fucoidan-shelled and genipin-crosslinked chitosan beads for antibacterial application.	0:100	Preparation of fucoidan-shelled and genipin-crosslinked chitosan beads for antibacterial application.
25933528	3	40	theme	gastric	474:480	arg1	pH					489:490	pH 1.2	489:494	pH 1.2	489:494	The drug release of the berberine-loaded beads was faster in simulated gastric fluid (pH 1.2) than those in simulated intestinal fluid (pH 7.4).
25933528	3	40	theme	gastric	474:480	arg1	fluid					482:486	simulated gastric fluid	464:486	simulated gastric fluid (pH 1.2)	464:495	The drug release of the berberine-loaded beads was faster in simulated gastric fluid (pH 1.2) than those in simulated intestinal fluid (pH 7.4).
25933528	6	41	theme	berberine-loaded	900:915	arg1	beads					917:921	the berberine-loaded beads	896:921	the berberine-loaded beads	896:921	Our results showed that the berberine-loaded beads and nanoparticles/beads complex could effectively inhibit the growth inhibition of common clinical pathogens, such as Staphylococcus aureus and Escherichia coli, and have the advantage of continually releasing berberine to inhibit the growth of the bacteria over 24 h.
25933528	1	42	theme	chitosan	136:143	arg1	bead					145:148	a fucoidan-shelled chitosan bead	117:148	a fucoidan-shelled chitosan bead	117:148	In this study, a fucoidan-shelled chitosan bead was developed with the purpose of oral delivery of berberine to inhibit the growth of bacteria.
25933528	6	43	theme	nanoparticles/beads	927:945	arg1	complex					947:953	nanoparticles/beads complex	927:953	nanoparticles/beads complex	927:953	Our results showed that the berberine-loaded beads and nanoparticles/beads complex could effectively inhibit the growth inhibition of common clinical pathogens, such as Staphylococcus aureus and Escherichia coli, and have the advantage of continually releasing berberine to inhibit the growth of the bacteria over 24 h.
25933528	4	44	theme	nanoparticles	667:679	arg1	incorporation					615:627	incorporation	615:627	incorporation of berberine-loaded chitosan/fucoidan nanoparticles in the fucoidan-shelled chitosan beads	615:718	Furthermore, a nanoparticles/beads complex system was developed by incorporation of berberine-loaded chitosan/fucoidan nanoparticles in the fucoidan-shelled chitosan beads.
25933528	2	45	theme	pH	326:327	arg1	value					329:333	the pH value	322:333	the pH value	322:333	The cross-linking level and swelling property of the beads were affected by the pH value and the composition of the genipin/fucoidan combined gelling agent.
25933528	4	46	theme	complex	583:589	arg1	system					591:596	a nanoparticles/beads complex system	561:596	a nanoparticles/beads complex system	561:596	Furthermore, a nanoparticles/beads complex system was developed by incorporation of berberine-loaded chitosan/fucoidan nanoparticles in the fucoidan-shelled chitosan beads.
25933528	3	47	theme	simulated	511:519	arg1	pH					539:540	pH 7.4	539:544	pH 7.4	539:544	The drug release of the berberine-loaded beads was faster in simulated gastric fluid (pH 1.2) than those in simulated intestinal fluid (pH 7.4).
25933528	3	47	theme	simulated	511:519	arg1	fluid					532:536	simulated intestinal fluid	511:536	simulated intestinal fluid (pH 7.4)	511:545	The drug release of the berberine-loaded beads was faster in simulated gastric fluid (pH 1.2) than those in simulated intestinal fluid (pH 7.4).
25933528	6	48	theme	berberine	1133:1141	arg1	advantage					1098:1106	the advantage	1094:1106	the advantage of continually releasing berberine to inhibit the growth of the bacteria over 24 h	1094:1189	Our results showed that the berberine-loaded beads and nanoparticles/beads complex could effectively inhibit the growth inhibition of common clinical pathogens, such as Staphylococcus aureus and Escherichia coli, and have the advantage of continually releasing berberine to inhibit the growth of the bacteria over 24 h.
25933528	3	49	theme	beads	444:448	arg1	faster					454:459	faster	454:459	faster	454:459	The drug release of the berberine-loaded beads was faster in simulated gastric fluid (pH 1.2) than those in simulated intestinal fluid (pH 7.4).
25933528	3	49	theme	beads	444:448	arg1	release					412:418	The drug release	403:418	The drug release of the berberine-loaded beads	403:448	The drug release of the berberine-loaded beads was faster in simulated gastric fluid (pH 1.2) than those in simulated intestinal fluid (pH 7.4).
25933528	2	50	theme	cross-linking	250:262	arg1	level					264:268	cross-linking level	250:268	cross-linking level	250:268	The cross-linking level and swelling property of the beads were affected by the pH value and the composition of the genipin/fucoidan combined gelling agent.
25933528	4	51	theme	fucoidan-shelled	688:703	arg1	beads					714:718	the fucoidan-shelled chitosan beads	684:718	the fucoidan-shelled chitosan beads	684:718	Furthermore, a nanoparticles/beads complex system was developed by incorporation of berberine-loaded chitosan/fucoidan nanoparticles in the fucoidan-shelled chitosan beads.
25933528	2	52	theme	gelling	388:394	arg1	agent					396:400	the genipin/fucoidan combined gelling agent	358:400	the genipin/fucoidan combined gelling agent	358:400	The cross-linking level and swelling property of the beads were affected by the pH value and the composition of the genipin/fucoidan combined gelling agent.
25933528	1	53	theme	bacteria	236:243	arg1	growth					226:231	the growth	222:231	the growth of bacteria	222:243	In this study, a fucoidan-shelled chitosan bead was developed with the purpose of oral delivery of berberine to inhibit the growth of bacteria.
25933528	0	54	theme	antibacterial	75:87	arg1	application					89:99	antibacterial application	75:99	antibacterial application	75:99	Preparation of fucoidan-shelled and genipin-crosslinked chitosan beads for antibacterial application.
25763036	5	0	theme	marine	665:670	arg1	bacterioplankton					672:687	marine bacterioplankton	665:687	marine bacterioplankton	665:687	Community composition of marine bacterioplankton was determined by 16S rRNA gene clone libraries.
25763036	3	1	theme	only	487:490	arg1	SW+OIL					515:520	SW+OIL	515:520	SW+OIL	515:520	Early hours of oil spill, were stimulated using an experimental seawater microcosm, supplemented with crude oil and EPS₂₀₀₃ (SW+OIL+EPS₂₀₀₃); this system was monitored for 2 days and compared to control microcosm (only oil-polluted seawater, SW+OIL).
25763036	3	1	theme	only	487:490	arg1	seawater					505:512	only oil-polluted seawater	487:512	only oil-polluted seawater	487:512	Early hours of oil spill, were stimulated using an experimental seawater microcosm, supplemented with crude oil and EPS₂₀₀₃ (SW+OIL+EPS₂₀₀₃); this system was monitored for 2 days and compared to control microcosm (only oil-polluted seawater, SW+OIL).
25763036	4	2	theme	cultivable	576:585	arg1	Determination					524:536	Determination	524:536	Determination of bacterial abundance, heterotrophic cultivable and hydrocarbon-degrading bacteria	524:620	Determination of bacterial abundance, heterotrophic cultivable and hydrocarbon-degrading bacteria were carried out.
25763036	3	3	theme	experimental	324:335	arg1	microcosm					346:354	an experimental seawater microcosm	321:354	an experimental seawater microcosm	321:354	Early hours of oil spill, were stimulated using an experimental seawater microcosm, supplemented with crude oil and EPS₂₀₀₃ (SW+OIL+EPS₂₀₀₃); this system was monitored for 2 days and compared to control microcosm (only oil-polluted seawater, SW+OIL).
25763036	2	4	theme	microcosms	109:118	arg1	experiments					120:130	Oil spill microcosms experiments	99:130	Oil spill microcosms experiments	99:130	Oil spill microcosms experiments were carried out to evaluate the effect of bioemulsificant exopolysaccharide (EPS₂₀₀₃) on quick stimulation of hydrocarbonoclastic bacteria.
25763036	5	5	theme	bacterioplankton	672:687	arg1	composition					650:660	Community composition	640:660	Community composition of marine bacterioplankton	640:687	Community composition of marine bacterioplankton was determined by 16S rRNA gene clone libraries.
25763036	3	6	theme	oil-polluted	492:503	arg1	SW+OIL					515:520	SW+OIL	515:520	SW+OIL	515:520	Early hours of oil spill, were stimulated using an experimental seawater microcosm, supplemented with crude oil and EPS₂₀₀₃ (SW+OIL+EPS₂₀₀₃); this system was monitored for 2 days and compared to control microcosm (only oil-polluted seawater, SW+OIL).
25763036	3	6	theme	oil-polluted	492:503	arg1	seawater					505:512	only oil-polluted seawater	487:512	only oil-polluted seawater	487:512	Early hours of oil spill, were stimulated using an experimental seawater microcosm, supplemented with crude oil and EPS₂₀₀₃ (SW+OIL+EPS₂₀₀₃); this system was monitored for 2 days and compared to control microcosm (only oil-polluted seawater, SW+OIL).
25763036	1	7	from	EPS₂₀₀₃	56:62	arg1	simulation					87:96	microcosm oil spill simulation	67:96	microcosm oil spill simulation	67:96	by bioemulsificant EPS₂₀₀₃ on microcosm oil spill simulation.
25763036	4	8	theme	heterotrophic	562:574	arg1	cultivable					576:585	heterotrophic cultivable	562:585	heterotrophic cultivable	562:585	Determination of bacterial abundance, heterotrophic cultivable and hydrocarbon-degrading bacteria were carried out.
25763036	2	9	theme	spill	103:107	arg1	experiments					120:130	Oil spill microcosms experiments	99:130	Oil spill microcosms experiments	99:130	Oil spill microcosms experiments were carried out to evaluate the effect of bioemulsificant exopolysaccharide (EPS₂₀₀₃) on quick stimulation of hydrocarbonoclastic bacteria.
25763036	3	10	dep	microcosm	476:484	arg1	SW+OIL					515:520	SW+OIL	515:520	SW+OIL	515:520	Early hours of oil spill, were stimulated using an experimental seawater microcosm, supplemented with crude oil and EPS₂₀₀₃ (SW+OIL+EPS₂₀₀₃); this system was monitored for 2 days and compared to control microcosm (only oil-polluted seawater, SW+OIL).
25763036	3	10	dep	microcosm	476:484	arg1	seawater					505:512	only oil-polluted seawater	487:512	only oil-polluted seawater	487:512	Early hours of oil spill, were stimulated using an experimental seawater microcosm, supplemented with crude oil and EPS₂₀₀₃ (SW+OIL+EPS₂₀₀₃); this system was monitored for 2 days and compared to control microcosm (only oil-polluted seawater, SW+OIL).
25763036	4	11	theme	hydrocarbon-degrading	591:611	arg1	bacteria					613:620	hydrocarbon-degrading bacteria	591:620	hydrocarbon-degrading bacteria	591:620	Determination of bacterial abundance, heterotrophic cultivable and hydrocarbon-degrading bacteria were carried out.
25763036	3	12	theme	crude	375:379	arg1	oil					381:383	crude oil	375:383	crude oil	375:383	Early hours of oil spill, were stimulated using an experimental seawater microcosm, supplemented with crude oil and EPS₂₀₀₃ (SW+OIL+EPS₂₀₀₃); this system was monitored for 2 days and compared to control microcosm (only oil-polluted seawater, SW+OIL).
25763036	6	13	dep	Alcanivorax	897:907	arg1	genus					909:913	Alcanivorax genus	897:913	Alcanivorax genus	897:913	Data obtained indicated that bioemulsificant addition stimulated an increase of total bacterial abundance and, in particular, selection of bacteria related to Alcanivorax genus; confirming that EPS₂₀₀₃ could be used for the dispersion of oil slicks and could stimulate the selection of marine hydrocarbon degraders thus increasing bioremediation process.
25763036	6	14	used	used	949:952	arg2	EPS₂₀₀₃					932:938	EPS₂₀₀₃	932:938	EPS₂₀₀₃	932:938	Data obtained indicated that bioemulsificant addition stimulated an increase of total bacterial abundance and, in particular, selection of bacteria related to Alcanivorax genus; confirming that EPS₂₀₀₃ could be used for the dispersion of oil slicks and could stimulate the selection of marine hydrocarbon degraders thus increasing bioremediation process.
25763036	1	15	theme	microcosm	67:75	arg1	simulation					87:96	microcosm oil spill simulation	67:96	microcosm oil spill simulation	67:96	by bioemulsificant EPS₂₀₀₃ on microcosm oil spill simulation.
25763036	3	16	theme	Early	273:277	arg1	hours					279:283	Early hours	273:283	Early hours	273:283	Early hours of oil spill, were stimulated using an experimental seawater microcosm, supplemented with crude oil and EPS₂₀₀₃ (SW+OIL+EPS₂₀₀₃); this system was monitored for 2 days and compared to control microcosm (only oil-polluted seawater, SW+OIL).
25763036	6	17	theme	abundance	834:842	arg1	selection					864:872	selection	864:872	selection of bacteria related to Alcanivorax genus	864:913	Data obtained indicated that bioemulsificant addition stimulated an increase of total bacterial abundance and, in particular, selection of bacteria related to Alcanivorax genus; confirming that EPS₂₀₀₃ could be used for the dispersion of oil slicks and could stimulate the selection of marine hydrocarbon degraders thus increasing bioremediation process.
25763036	6	17	theme	abundance	834:842	arg1	increase					806:813	an increase	803:813	an increase of total bacterial abundance	803:842	Data obtained indicated that bioemulsificant addition stimulated an increase of total bacterial abundance and, in particular, selection of bacteria related to Alcanivorax genus; confirming that EPS₂₀₀₃ could be used for the dispersion of oil slicks and could stimulate the selection of marine hydrocarbon degraders thus increasing bioremediation process.
25763036	1	18	theme	oil	77:79	arg1	simulation					87:96	microcosm oil spill simulation	67:96	microcosm oil spill simulation	67:96	by bioemulsificant EPS₂₀₀₃ on microcosm oil spill simulation.
25763036	6	19	theme	slicks	980:985	arg1	dispersion					962:971	the dispersion	958:971	the dispersion of oil slicks	958:985	Data obtained indicated that bioemulsificant addition stimulated an increase of total bacterial abundance and, in particular, selection of bacteria related to Alcanivorax genus; confirming that EPS₂₀₀₃ could be used for the dispersion of oil slicks and could stimulate the selection of marine hydrocarbon degraders thus increasing bioremediation process.
25763036	2	20	from	effect	165:170	arg1	stimulation					228:238	quick stimulation	222:238	quick stimulation of hydrocarbonoclastic bacteria	222:270	Oil spill microcosms experiments were carried out to evaluate the effect of bioemulsificant exopolysaccharide (EPS₂₀₀₃) on quick stimulation of hydrocarbonoclastic bacteria.
25763036	1	21	theme	spill	81:85	arg1	simulation					87:96	microcosm oil spill simulation	67:96	microcosm oil spill simulation	67:96	by bioemulsificant EPS₂₀₀₃ on microcosm oil spill simulation.
25763036	0	22	theme	Quick	0:4	arg1	stimulation					6:16	Quick stimulation	0:16	Quick stimulation of Alcanivorax sp	0:34	Quick stimulation of Alcanivorax sp.
25763036	4	23	theme	bacterial	541:549	arg1	abundance					551:559	bacterial abundance	541:559	bacterial abundance	541:559	Determination of bacterial abundance, heterotrophic cultivable and hydrocarbon-degrading bacteria were carried out.
25763036	6	24	theme	total	818:822	arg1	abundance					834:842	total bacterial abundance	818:842	total bacterial abundance	818:842	Data obtained indicated that bioemulsificant addition stimulated an increase of total bacterial abundance and, in particular, selection of bacteria related to Alcanivorax genus; confirming that EPS₂₀₀₃ could be used for the dispersion of oil slicks and could stimulate the selection of marine hydrocarbon degraders thus increasing bioremediation process.
25763036	6	25	theme	marine	1024:1029	arg1	degraders					1043:1051	marine hydrocarbon degraders	1024:1051	marine hydrocarbon degraders	1024:1051	Data obtained indicated that bioemulsificant addition stimulated an increase of total bacterial abundance and, in particular, selection of bacteria related to Alcanivorax genus; confirming that EPS₂₀₀₃ could be used for the dispersion of oil slicks and could stimulate the selection of marine hydrocarbon degraders thus increasing bioremediation process.
25763036	0	26	theme	Alcanivorax	21:31	arg1	sp					33:34	Alcanivorax sp	21:34	Alcanivorax sp	21:34	Quick stimulation of Alcanivorax sp.
25763036	3	27	theme	oil	288:290	arg1	spill					292:296	oil spill	288:296	oil spill	288:296	Early hours of oil spill, were stimulated using an experimental seawater microcosm, supplemented with crude oil and EPS₂₀₀₃ (SW+OIL+EPS₂₀₀₃); this system was monitored for 2 days and compared to control microcosm (only oil-polluted seawater, SW+OIL).
25763036	3	28	theme	spill	292:296	arg1	hours					279:283	Early hours	273:283	Early hours	273:283	Early hours of oil spill, were stimulated using an experimental seawater microcosm, supplemented with crude oil and EPS₂₀₀₃ (SW+OIL+EPS₂₀₀₃); this system was monitored for 2 days and compared to control microcosm (only oil-polluted seawater, SW+OIL).
25763036	2	29	theme	bioemulsificant	175:189	arg1	EPS₂₀₀₃					210:216	bioemulsificant exopolysaccharide (EPS₂₀₀₃)	175:217	bioemulsificant exopolysaccharide (EPS₂₀₀₃)	175:217	Oil spill microcosms experiments were carried out to evaluate the effect of bioemulsificant exopolysaccharide (EPS₂₀₀₃) on quick stimulation of hydrocarbonoclastic bacteria.
25763036	4	30	theme	abundance	551:559	arg1	Determination					524:536	Determination	524:536	Determination of bacterial abundance, heterotrophic cultivable and hydrocarbon-degrading bacteria	524:620	Determination of bacterial abundance, heterotrophic cultivable and hydrocarbon-degrading bacteria were carried out.
25763036	0	31	theme	sp	33:34	arg1	stimulation					6:16	Quick stimulation	0:16	Quick stimulation of Alcanivorax sp	0:34	Quick stimulation of Alcanivorax sp.
25763036	2	32	theme	Oil	99:101	arg1	experiments					120:130	Oil spill microcosms experiments	99:130	Oil spill microcosms experiments	99:130	Oil spill microcosms experiments were carried out to evaluate the effect of bioemulsificant exopolysaccharide (EPS₂₀₀₃) on quick stimulation of hydrocarbonoclastic bacteria.
25763036	6	33	theme	bioremediation	1069:1082	arg1	process					1084:1090	bioremediation process	1069:1090	bioremediation process	1069:1090	Data obtained indicated that bioemulsificant addition stimulated an increase of total bacterial abundance and, in particular, selection of bacteria related to Alcanivorax genus; confirming that EPS₂₀₀₃ could be used for the dispersion of oil slicks and could stimulate the selection of marine hydrocarbon degraders thus increasing bioremediation process.
25763036	6	34	theme	oil	976:978	arg1	slicks					980:985	oil slicks	976:985	oil slicks	976:985	Data obtained indicated that bioemulsificant addition stimulated an increase of total bacterial abundance and, in particular, selection of bacteria related to Alcanivorax genus; confirming that EPS₂₀₀₃ could be used for the dispersion of oil slicks and could stimulate the selection of marine hydrocarbon degraders thus increasing bioremediation process.
25763036	6	35	theme	related	886:892	arg1	bacteria					877:884	bacteria	877:884	bacteria related to Alcanivorax genus	877:913	Data obtained indicated that bioemulsificant addition stimulated an increase of total bacterial abundance and, in particular, selection of bacteria related to Alcanivorax genus; confirming that EPS₂₀₀₃ could be used for the dispersion of oil slicks and could stimulate the selection of marine hydrocarbon degraders thus increasing bioremediation process.
25763036	2	36	theme	quick	222:226	arg1	stimulation					228:238	quick stimulation	222:238	quick stimulation of hydrocarbonoclastic bacteria	222:270	Oil spill microcosms experiments were carried out to evaluate the effect of bioemulsificant exopolysaccharide (EPS₂₀₀₃) on quick stimulation of hydrocarbonoclastic bacteria.
25763036	5	37	theme	clone	721:725	arg1	libraries					727:735	rRNA gene clone libraries	711:735	rRNA gene clone libraries	711:735	Community composition of marine bacterioplankton was determined by 16S rRNA gene clone libraries.
25763036	6	38	theme	bacteria	877:884	arg1	selection					864:872	selection	864:872	selection of bacteria related to Alcanivorax genus	864:913	Data obtained indicated that bioemulsificant addition stimulated an increase of total bacterial abundance and, in particular, selection of bacteria related to Alcanivorax genus; confirming that EPS₂₀₀₃ could be used for the dispersion of oil slicks and could stimulate the selection of marine hydrocarbon degraders thus increasing bioremediation process.
25763036	6	38	theme	bacteria	877:884	arg1	increase					806:813	an increase	803:813	an increase of total bacterial abundance	803:842	Data obtained indicated that bioemulsificant addition stimulated an increase of total bacterial abundance and, in particular, selection of bacteria related to Alcanivorax genus; confirming that EPS₂₀₀₃ could be used for the dispersion of oil slicks and could stimulate the selection of marine hydrocarbon degraders thus increasing bioremediation process.
25763036	5	39	theme	Community	640:648	arg1	composition					650:660	Community composition	640:660	Community composition of marine bacterioplankton	640:687	Community composition of marine bacterioplankton was determined by 16S rRNA gene clone libraries.
25763036	6	40	theme	bacterial	824:832	arg1	abundance					834:842	total bacterial abundance	818:842	total bacterial abundance	818:842	Data obtained indicated that bioemulsificant addition stimulated an increase of total bacterial abundance and, in particular, selection of bacteria related to Alcanivorax genus; confirming that EPS₂₀₀₃ could be used for the dispersion of oil slicks and could stimulate the selection of marine hydrocarbon degraders thus increasing bioremediation process.
25763036	1	41	theme	bioemulsificant	40:54	arg1	EPS₂₀₀₃					56:62	bioemulsificant EPS₂₀₀₃	40:62	bioemulsificant EPS₂₀₀₃ on microcosm oil spill simulation	40:96	by bioemulsificant EPS₂₀₀₃ on microcosm oil spill simulation.
25763036	5	42	theme	rRNA	711:714	arg1	libraries					727:735	rRNA gene clone libraries	711:735	rRNA gene clone libraries	711:735	Community composition of marine bacterioplankton was determined by 16S rRNA gene clone libraries.
25763036	2	43	theme	EPS₂₀₀₃	210:216	arg1	effect					165:170	the effect	161:170	the effect of bioemulsificant exopolysaccharide (EPS₂₀₀₃) on quick stimulation of hydrocarbonoclastic bacteria	161:270	Oil spill microcosms experiments were carried out to evaluate the effect of bioemulsificant exopolysaccharide (EPS₂₀₀₃) on quick stimulation of hydrocarbonoclastic bacteria.
25763036	5	44	theme	gene	716:719	arg1	libraries					727:735	rRNA gene clone libraries	711:735	rRNA gene clone libraries	711:735	Community composition of marine bacterioplankton was determined by 16S rRNA gene clone libraries.
25763036	6	45	theme	bioemulsificant	767:781	arg1	addition					783:790	bioemulsificant addition	767:790	bioemulsificant addition	767:790	Data obtained indicated that bioemulsificant addition stimulated an increase of total bacterial abundance and, in particular, selection of bacteria related to Alcanivorax genus; confirming that EPS₂₀₀₃ could be used for the dispersion of oil slicks and could stimulate the selection of marine hydrocarbon degraders thus increasing bioremediation process.
25763036	3	46	theme	control	468:474	arg1	microcosm					476:484	control microcosm	468:484	control microcosm (only oil-polluted seawater, SW+OIL)	468:521	Early hours of oil spill, were stimulated using an experimental seawater microcosm, supplemented with crude oil and EPS₂₀₀₃ (SW+OIL+EPS₂₀₀₃); this system was monitored for 2 days and compared to control microcosm (only oil-polluted seawater, SW+OIL).
25763036	4	47	theme	bacteria	613:620	arg1	Determination					524:536	Determination	524:536	Determination of bacterial abundance, heterotrophic cultivable and hydrocarbon-degrading bacteria	524:620	Determination of bacterial abundance, heterotrophic cultivable and hydrocarbon-degrading bacteria were carried out.
25763036	3	48	theme	seawater	337:344	arg1	microcosm					346:354	an experimental seawater microcosm	321:354	an experimental seawater microcosm	321:354	Early hours of oil spill, were stimulated using an experimental seawater microcosm, supplemented with crude oil and EPS₂₀₀₃ (SW+OIL+EPS₂₀₀₃); this system was monitored for 2 days and compared to control microcosm (only oil-polluted seawater, SW+OIL).
25763036	2	49	theme	exopolysaccharide	191:207	arg1	EPS₂₀₀₃					210:216	bioemulsificant exopolysaccharide (EPS₂₀₀₃)	175:217	bioemulsificant exopolysaccharide (EPS₂₀₀₃)	175:217	Oil spill microcosms experiments were carried out to evaluate the effect of bioemulsificant exopolysaccharide (EPS₂₀₀₃) on quick stimulation of hydrocarbonoclastic bacteria.
25763036	2	50	theme	bacteria	263:270	arg1	stimulation					228:238	quick stimulation	222:238	quick stimulation of hydrocarbonoclastic bacteria	222:270	Oil spill microcosms experiments were carried out to evaluate the effect of bioemulsificant exopolysaccharide (EPS₂₀₀₃) on quick stimulation of hydrocarbonoclastic bacteria.
25763036	6	51	theme	degraders	1043:1051	arg1	selection					1011:1019	the selection	1007:1019	the selection of marine hydrocarbon degraders	1007:1051	Data obtained indicated that bioemulsificant addition stimulated an increase of total bacterial abundance and, in particular, selection of bacteria related to Alcanivorax genus; confirming that EPS₂₀₀₃ could be used for the dispersion of oil slicks and could stimulate the selection of marine hydrocarbon degraders thus increasing bioremediation process.
25763036	2	52	theme	hydrocarbonoclastic	243:261	arg1	bacteria					263:270	hydrocarbonoclastic bacteria	243:270	hydrocarbonoclastic bacteria	243:270	Oil spill microcosms experiments were carried out to evaluate the effect of bioemulsificant exopolysaccharide (EPS₂₀₀₃) on quick stimulation of hydrocarbonoclastic bacteria.
25763036	6	53	theme	hydrocarbon	1031:1041	arg1	degraders					1043:1051	marine hydrocarbon degraders	1024:1051	marine hydrocarbon degraders	1024:1051	Data obtained indicated that bioemulsificant addition stimulated an increase of total bacterial abundance and, in particular, selection of bacteria related to Alcanivorax genus; confirming that EPS₂₀₀₃ could be used for the dispersion of oil slicks and could stimulate the selection of marine hydrocarbon degraders thus increasing bioremediation process.
25828039	8	0	theme	JUV	1169:1171	arg1	nature					1154:1159	nature	1154:1159	nature of BEL, JUV, and RES	1154:1180	CONCLUSION Descriptions of the ultrastructural compositions and nature of BEL, JUV, and RES are helpful for matching the areas to be treated with the HA soft-tissue filler architecture.
25828039	8	0	theme	JUV	1169:1171	arg1	Descriptions					1101:1112	CONCLUSION Descriptions	1090:1112	CONCLUSION Descriptions of the ultrastructural compositions	1090:1148	CONCLUSION Descriptions of the ultrastructural compositions and nature of BEL, JUV, and RES are helpful for matching the areas to be treated with the HA soft-tissue filler architecture.
25828039	2	1	theme	HA	385:386	arg1	structure					388:396	HA structure	385:396	HA structure	385:396	OBJECTIVE To examine uniformity of HA structure, the effects of extrusion, and lidocaine dilution of 3 commercially available HA soft-tissue fillers.
25828039	1	2	theme	HA	257:258	arg1	characteristics					292:306	HA ultrastructural and morphologic characteristics	257:306	HA ultrastructural and morphologic characteristics	257:306	BACKGROUND Although hyaluronic acid (HA) specifications such as molecular weight and particle size are fairly well characterized, little information about HA ultrastructural and morphologic characteristics has been reported in clinical literature.
25828039	0	3	theme	electron	81:88	arg1	microscopy					90:99	scanning electron microscopy	72:99	scanning electron microscopy	72:99	Ultrastructural analysis of 3 hyaluronic acid soft-tissue fillers using scanning electron microscopy.
25828039	9	4	theme	30-G	1310:1313	arg1	needle					1315:1320	a 30-G needle	1308:1320	a 30-G needle	1308:1320	Lidocaine and extrusion through a 30-G needle exerted no influence on HA structure.
25828039	5	5	located	present	842:848	arg2	matter					831:836	Irregular particulate matter	809:836	Irregular particulate matter	809:836	Irregular particulate matter was present in RES but BEL and JUV were largely particle free.
25828039	5	5	located	present	842:848	arg1	RES					853:855	RES	853:855	RES	853:855	Irregular particulate matter was present in RES but BEL and JUV were largely particle free.
25828039	7	6	theme	apparent	994:1001	arg1	effect					1003:1008	no apparent effect	991:1008	no apparent effect	991:1008	Lidocaine had no apparent effect on morphology; extrusion through a 30-G needle had no effect on ultrastructure.
25828039	1	7	theme	molecular	166:174	arg1	weight					176:181	molecular weight	166:181	molecular weight	166:181	BACKGROUND Although hyaluronic acid (HA) specifications such as molecular weight and particle size are fairly well characterized, little information about HA ultrastructural and morphologic characteristics has been reported in clinical literature.
25828039	8	8	theme	soft-tissue	1243:1253	arg1	architecture					1262:1273	the HA soft-tissue filler architecture	1236:1273	the HA soft-tissue filler architecture	1236:1273	CONCLUSION Descriptions of the ultrastructural compositions and nature of BEL, JUV, and RES are helpful for matching the areas to be treated with the HA soft-tissue filler architecture.
25828039	1	9	theme	ultrastructural	260:274	arg1	characteristics					292:306	HA ultrastructural and morphologic characteristics	257:306	HA ultrastructural and morphologic characteristics	257:306	BACKGROUND Although hyaluronic acid (HA) specifications such as molecular weight and particle size are fairly well characterized, little information about HA ultrastructural and morphologic characteristics has been reported in clinical literature.
25828039	7	10	contain	had	987:989	arg2	effect					1003:1008	no apparent effect	991:1008	no apparent effect	991:1008	Lidocaine had no apparent effect on morphology; extrusion through a 30-G needle had no effect on ultrastructure.
25828039	7	10	contain	had	987:989	arg1	Lidocaine					977:985	Lidocaine	977:985	Lidocaine	977:985	Lidocaine had no apparent effect on morphology; extrusion through a 30-G needle had no effect on ultrastructure.
25828039	5	11	from	RES	853:855	arg1	present					842:848	present	842:848	present	842:848	Irregular particulate matter was present in RES but BEL and JUV were largely particle free.
25828039	6	12	from	BEL	929:931	arg1	uniform					918:924	uniform	918:924	uniform	918:924	Spacing was more uniform in BEL than JUV and JUV was more uniform than RES.
25828039	3	13	theme	scanning	528:535	arg1	microscopy					546:555	scanning electron microscopy	528:555	scanning electron microscopy	528:555	MATERIALS AND METHODS Using scanning electron microscopy and energy-dispersive x-ray analysis, investigators examined the soft-tissue fillers at various magnifications for ultrastructural detail and elemental distributions.
25828039	1	14	theme	morphologic	280:290	arg1	characteristics					292:306	HA ultrastructural and morphologic characteristics	257:306	HA ultrastructural and morphologic characteristics	257:306	BACKGROUND Although hyaluronic acid (HA) specifications such as molecular weight and particle size are fairly well characterized, little information about HA ultrastructural and morphologic characteristics has been reported in clinical literature.
25828039	3	15	theme	energy-dispersive	561:577	arg1	analysis					585:592	energy-dispersive x-ray analysis	561:592	energy-dispersive x-ray analysis	561:592	MATERIALS AND METHODS Using scanning electron microscopy and energy-dispersive x-ray analysis, investigators examined the soft-tissue fillers at various magnifications for ultrastructural detail and elemental distributions.
25828039	7	16	contain	had	1057:1059	arg1	extrusion					1025:1033	extrusion	1025:1033	extrusion through a 30-G needle	1025:1055	Lidocaine had no apparent effect on morphology; extrusion through a 30-G needle had no effect on ultrastructure.
25828039	7	16	contain	had	1057:1059	arg2	effect					1064:1069	no effect	1061:1069	no effect	1061:1069	Lidocaine had no apparent effect on morphology; extrusion through a 30-G needle had no effect on ultrastructure.
25828039	2	17	theme	extrusion	414:422	arg1	uniformity					371:380	uniformity	371:380	uniformity of HA structure	371:396	OBJECTIVE To examine uniformity of HA structure, the effects of extrusion, and lidocaine dilution of 3 commercially available HA soft-tissue fillers.
25828039	2	17	theme	extrusion	414:422	arg1	effects					403:409	the effects	399:409	the effects of extrusion	399:422	OBJECTIVE To examine uniformity of HA structure, the effects of extrusion, and lidocaine dilution of 3 commercially available HA soft-tissue fillers.
25828039	2	17	theme	extrusion	414:422	arg1	dilution					439:446	lidocaine dilution	429:446	lidocaine dilution of 3 commercially available HA soft-tissue fillers	429:497	OBJECTIVE To examine uniformity of HA structure, the effects of extrusion, and lidocaine dilution of 3 commercially available HA soft-tissue fillers.
25828039	2	18	theme	HA	476:477	arg1	fillers					491:497	3 commercially available HA soft-tissue fillers	451:497	3 commercially available HA soft-tissue fillers	451:497	OBJECTIVE To examine uniformity of HA structure, the effects of extrusion, and lidocaine dilution of 3 commercially available HA soft-tissue fillers.
25828039	3	19	theme	x-ray	579:583	arg1	analysis					585:592	energy-dispersive x-ray analysis	561:592	energy-dispersive x-ray analysis	561:592	MATERIALS AND METHODS Using scanning electron microscopy and energy-dispersive x-ray analysis, investigators examined the soft-tissue fillers at various magnifications for ultrastructural detail and elemental distributions.
25828039	8	20	theme	HA	1240:1241	arg1	architecture					1262:1273	the HA soft-tissue filler architecture	1236:1273	the HA soft-tissue filler architecture	1236:1273	CONCLUSION Descriptions of the ultrastructural compositions and nature of BEL, JUV, and RES are helpful for matching the areas to be treated with the HA soft-tissue filler architecture.
25828039	2	21	theme	available	466:474	arg1	fillers					491:497	3 commercially available HA soft-tissue fillers	451:497	3 commercially available HA soft-tissue fillers	451:497	OBJECTIVE To examine uniformity of HA structure, the effects of extrusion, and lidocaine dilution of 3 commercially available HA soft-tissue fillers.
25828039	5	22	theme	particulate	819:829	arg1	matter					831:836	Irregular particulate matter	809:836	Irregular particulate matter	809:836	Irregular particulate matter was present in RES but BEL and JUV were largely particle free.
25828039	1	23	theme	particle	187:194	arg1	size					196:199	particle size	187:199	particle size	187:199	BACKGROUND Although hyaluronic acid (HA) specifications such as molecular weight and particle size are fairly well characterized, little information about HA ultrastructural and morphologic characteristics has been reported in clinical literature.
25828039	0	24	theme	Ultrastructural	0:14	arg1	analysis					16:23	Ultrastructural analysis	0:23	Ultrastructural analysis of 3 hyaluronic acid soft-tissue fillers using scanning electron microscopy.	0:100	Ultrastructural analysis of 3 hyaluronic acid soft-tissue fillers using scanning electron microscopy.
25828039	9	25	theme	HA	1346:1347	arg1	structure					1349:1357	HA structure	1346:1357	HA structure	1346:1357	Lidocaine and extrusion through a 30-G needle exerted no influence on HA structure.
25828039	8	26	theme	CONCLUSION	1090:1099	arg1	Descriptions					1101:1112	CONCLUSION Descriptions	1090:1112	CONCLUSION Descriptions of the ultrastructural compositions	1090:1148	CONCLUSION Descriptions of the ultrastructural compositions and nature of BEL, JUV, and RES are helpful for matching the areas to be treated with the HA soft-tissue filler architecture.
25828039	3	27	theme	soft-tissue	622:632	arg1	fillers					634:640	the soft-tissue fillers	618:640	the soft-tissue fillers at various magnifications for ultrastructural detail and elemental distributions	618:721	MATERIALS AND METHODS Using scanning electron microscopy and energy-dispersive x-ray analysis, investigators examined the soft-tissue fillers at various magnifications for ultrastructural detail and elemental distributions.
25828039	8	28	theme	filler	1255:1260	arg1	architecture					1262:1273	the HA soft-tissue filler architecture	1236:1273	the HA soft-tissue filler architecture	1236:1273	CONCLUSION Descriptions of the ultrastructural compositions and nature of BEL, JUV, and RES are helpful for matching the areas to be treated with the HA soft-tissue filler architecture.
25828039	10	29	theme	manufactured	1429:1440	arg1	lots					1442:1445	manufactured lots	1429:1445	manufactured lots	1429:1445	Belotero Balance shows consistency throughout the syringe and across manufactured lots.
25828039	4	30	dep	RESULTS	724:730	arg1	All					732:734	All	732:734	All	732:734	RESULTS All HAs contained oxygen, carbon, and sodium, but with uneven distributions.
25828039	4	31	theme	uneven	787:792	arg1	distributions					794:806	uneven distributions	787:806	uneven distributions	787:806	RESULTS All HAs contained oxygen, carbon, and sodium, but with uneven distributions.
25828039	5	32	attach	present	842:848	arg2	matter					831:836	Irregular particulate matter	809:836	Irregular particulate matter	809:836	Irregular particulate matter was present in RES but BEL and JUV were largely particle free.
25828039	5	32	attach	present	842:848	arg1	RES					853:855	RES	853:855	RES	853:855	Irregular particulate matter was present in RES but BEL and JUV were largely particle free.
25828039	1	33	theme	hyaluronic	122:131	arg1	HA					139:140	HA	139:140	HA	139:140	BACKGROUND Although hyaluronic acid (HA) specifications such as molecular weight and particle size are fairly well characterized, little information about HA ultrastructural and morphologic characteristics has been reported in clinical literature.
25828039	1	33	theme	hyaluronic	122:131	arg1	acid					133:136	hyaluronic acid	122:136	hyaluronic acid (HA) specifications such as molecular weight and particle size	122:199	BACKGROUND Although hyaluronic acid (HA) specifications such as molecular weight and particle size are fairly well characterized, little information about HA ultrastructural and morphologic characteristics has been reported in clinical literature.
25828039	2	34	theme	lidocaine	429:437	arg1	dilution					439:446	lidocaine dilution	429:446	lidocaine dilution of 3 commercially available HA soft-tissue fillers	429:497	OBJECTIVE To examine uniformity of HA structure, the effects of extrusion, and lidocaine dilution of 3 commercially available HA soft-tissue fillers.
25828039	0	35	theme	acid	41:44	arg1	fillers					58:64	3 hyaluronic acid soft-tissue fillers	28:64	3 hyaluronic acid soft-tissue fillers	28:64	Ultrastructural analysis of 3 hyaluronic acid soft-tissue fillers using scanning electron microscopy.
25828039	8	36	theme	compositions	1137:1148	arg1	nature					1154:1159	nature	1154:1159	nature of BEL, JUV, and RES	1154:1180	CONCLUSION Descriptions of the ultrastructural compositions and nature of BEL, JUV, and RES are helpful for matching the areas to be treated with the HA soft-tissue filler architecture.
25828039	8	36	theme	compositions	1137:1148	arg1	Descriptions					1101:1112	CONCLUSION Descriptions	1090:1112	CONCLUSION Descriptions of the ultrastructural compositions	1090:1148	CONCLUSION Descriptions of the ultrastructural compositions and nature of BEL, JUV, and RES are helpful for matching the areas to be treated with the HA soft-tissue filler architecture.
25828039	3	37	theme	various	645:651	arg1	magnifications					653:666	various magnifications	645:666	various magnifications	645:666	MATERIALS AND METHODS Using scanning electron microscopy and energy-dispersive x-ray analysis, investigators examined the soft-tissue fillers at various magnifications for ultrastructural detail and elemental distributions.
25828039	3	38	from	magnifications	653:666	arg1	fillers					634:640	the soft-tissue fillers	618:640	the soft-tissue fillers at various magnifications for ultrastructural detail and elemental distributions	618:721	MATERIALS AND METHODS Using scanning electron microscopy and energy-dispersive x-ray analysis, investigators examined the soft-tissue fillers at various magnifications for ultrastructural detail and elemental distributions.
25828039	2	39	theme	fillers	491:497	arg1	uniformity					371:380	uniformity	371:380	uniformity of HA structure	371:396	OBJECTIVE To examine uniformity of HA structure, the effects of extrusion, and lidocaine dilution of 3 commercially available HA soft-tissue fillers.
25828039	2	39	theme	fillers	491:497	arg1	effects					403:409	the effects	399:409	the effects of extrusion	399:422	OBJECTIVE To examine uniformity of HA structure, the effects of extrusion, and lidocaine dilution of 3 commercially available HA soft-tissue fillers.
25828039	2	39	theme	fillers	491:497	arg1	dilution					439:446	lidocaine dilution	429:446	lidocaine dilution of 3 commercially available HA soft-tissue fillers	429:497	OBJECTIVE To examine uniformity of HA structure, the effects of extrusion, and lidocaine dilution of 3 commercially available HA soft-tissue fillers.
25828039	0	40	theme	hyaluronic	30:39	arg1	fillers					58:64	3 hyaluronic acid soft-tissue fillers	28:64	3 hyaluronic acid soft-tissue fillers	28:64	Ultrastructural analysis of 3 hyaluronic acid soft-tissue fillers using scanning electron microscopy.
25828039	3	41	theme	elemental	699:707	arg1	distributions					709:721	elemental distributions	699:721	elemental distributions	699:721	MATERIALS AND METHODS Using scanning electron microscopy and energy-dispersive x-ray analysis, investigators examined the soft-tissue fillers at various magnifications for ultrastructural detail and elemental distributions.
25828039	7	42	theme	30-G	1045:1048	arg1	needle					1050:1055	a 30-G needle	1043:1055	a 30-G needle	1043:1055	Lidocaine had no apparent effect on morphology; extrusion through a 30-G needle had no effect on ultrastructure.
25828039	5	43	theme	Irregular	809:817	arg1	matter					831:836	Irregular particulate matter	809:836	Irregular particulate matter	809:836	Irregular particulate matter was present in RES but BEL and JUV were largely particle free.
25828039	0	44	theme	fillers	58:64	arg1	analysis					16:23	Ultrastructural analysis	0:23	Ultrastructural analysis of 3 hyaluronic acid soft-tissue fillers using scanning electron microscopy.	0:100	Ultrastructural analysis of 3 hyaluronic acid soft-tissue fillers using scanning electron microscopy.
25828039	1	45	theme	clinical	329:336	arg1	literature					338:347	clinical literature	329:347	clinical literature	329:347	BACKGROUND Although hyaluronic acid (HA) specifications such as molecular weight and particle size are fairly well characterized, little information about HA ultrastructural and morphologic characteristics has been reported in clinical literature.
25828039	1	46	theme	acid	133:136	arg1	weight					176:181	molecular weight	166:181	molecular weight	166:181	BACKGROUND Although hyaluronic acid (HA) specifications such as molecular weight and particle size are fairly well characterized, little information about HA ultrastructural and morphologic characteristics has been reported in clinical literature.
25828039	1	46	theme	acid	133:136	arg1	size					196:199	particle size	187:199	particle size	187:199	BACKGROUND Although hyaluronic acid (HA) specifications such as molecular weight and particle size are fairly well characterized, little information about HA ultrastructural and morphologic characteristics has been reported in clinical literature.
25828039	1	46	theme	acid	133:136	arg1	specifications					143:156	hyaluronic acid (HA) specifications	122:156	hyaluronic acid (HA) specifications such as molecular weight and particle size	122:199	BACKGROUND Although hyaluronic acid (HA) specifications such as molecular weight and particle size are fairly well characterized, little information about HA ultrastructural and morphologic characteristics has been reported in clinical literature.
25828039	0	47	theme	soft-tissue	46:56	arg1	fillers					58:64	3 hyaluronic acid soft-tissue fillers	28:64	3 hyaluronic acid soft-tissue fillers	28:64	Ultrastructural analysis of 3 hyaluronic acid soft-tissue fillers using scanning electron microscopy.
25828039	4	48	contain	contained	740:748	arg2	oxygen					750:755	oxygen	750:755	oxygen	750:755	RESULTS All HAs contained oxygen, carbon, and sodium, but with uneven distributions.
25828039	4	48	contain	contained	740:748	arg1	RESULTS					724:730	RESULTS	724:730	RESULTS All HAs	724:738	RESULTS All HAs contained oxygen, carbon, and sodium, but with uneven distributions.
25828039	4	48	contain	contained	740:748	arg2	sodium					770:775	sodium	770:775	sodium	770:775	RESULTS All HAs contained oxygen, carbon, and sodium, but with uneven distributions.
25828039	4	48	contain	contained	740:748	arg2	carbon					758:763	carbon	758:763	carbon	758:763	RESULTS All HAs contained oxygen, carbon, and sodium, but with uneven distributions.
25828039	3	49	theme	electron	537:544	arg1	microscopy					546:555	scanning electron microscopy	528:555	scanning electron microscopy	528:555	MATERIALS AND METHODS Using scanning electron microscopy and energy-dispersive x-ray analysis, investigators examined the soft-tissue fillers at various magnifications for ultrastructural detail and elemental distributions.
25828039	4	50	dep	All	732:734	arg1	HAs					736:738	HAs	736:738	HAs	736:738	RESULTS All HAs contained oxygen, carbon, and sodium, but with uneven distributions.
25828039	8	51	theme	BEL	1164:1166	arg1	nature					1154:1159	nature	1154:1159	nature of BEL, JUV, and RES	1154:1180	CONCLUSION Descriptions of the ultrastructural compositions and nature of BEL, JUV, and RES are helpful for matching the areas to be treated with the HA soft-tissue filler architecture.
25828039	8	51	theme	BEL	1164:1166	arg1	Descriptions					1101:1112	CONCLUSION Descriptions	1090:1112	CONCLUSION Descriptions of the ultrastructural compositions	1090:1148	CONCLUSION Descriptions of the ultrastructural compositions and nature of BEL, JUV, and RES are helpful for matching the areas to be treated with the HA soft-tissue filler architecture.
25828039	5	52	from	present	842:848	arg1	RES					853:855	RES	853:855	RES	853:855	Irregular particulate matter was present in RES but BEL and JUV were largely particle free.
25828039	6	53	from	uniform	918:924	arg1	BEL					929:931	BEL	929:931	BEL	929:931	Spacing was more uniform in BEL than JUV and JUV was more uniform than RES.
25828039	10	54	theme	Belotero	1360:1367	arg1	Balance					1369:1375	Belotero Balance	1360:1375	Belotero Balance	1360:1375	Belotero Balance shows consistency throughout the syringe and across manufactured lots.
25828039	1	55	theme	little	232:237	arg1	information					239:249	little information	232:249	little information about HA ultrastructural and morphologic characteristics	232:306	BACKGROUND Although hyaluronic acid (HA) specifications such as molecular weight and particle size are fairly well characterized, little information about HA ultrastructural and morphologic characteristics has been reported in clinical literature.
25828039	0	56	theme	scanning	72:79	arg1	microscopy					90:99	scanning electron microscopy	72:99	scanning electron microscopy	72:99	Ultrastructural analysis of 3 hyaluronic acid soft-tissue fillers using scanning electron microscopy.
25828039	2	57	theme	soft-tissue	479:489	arg1	fillers					491:497	3 commercially available HA soft-tissue fillers	451:497	3 commercially available HA soft-tissue fillers	451:497	OBJECTIVE To examine uniformity of HA structure, the effects of extrusion, and lidocaine dilution of 3 commercially available HA soft-tissue fillers.
25828039	3	58	theme	ultrastructural	672:686	arg1	detail					688:693	ultrastructural detail	672:693	ultrastructural detail	672:693	MATERIALS AND METHODS Using scanning electron microscopy and energy-dispersive x-ray analysis, investigators examined the soft-tissue fillers at various magnifications for ultrastructural detail and elemental distributions.
25828039	2	59	theme	structure	388:396	arg1	uniformity					371:380	uniformity	371:380	uniformity of HA structure	371:396	OBJECTIVE To examine uniformity of HA structure, the effects of extrusion, and lidocaine dilution of 3 commercially available HA soft-tissue fillers.
25828039	2	59	theme	structure	388:396	arg1	effects					403:409	the effects	399:409	the effects of extrusion	399:422	OBJECTIVE To examine uniformity of HA structure, the effects of extrusion, and lidocaine dilution of 3 commercially available HA soft-tissue fillers.
25828039	2	59	theme	structure	388:396	arg1	dilution					439:446	lidocaine dilution	429:446	lidocaine dilution of 3 commercially available HA soft-tissue fillers	429:497	OBJECTIVE To examine uniformity of HA structure, the effects of extrusion, and lidocaine dilution of 3 commercially available HA soft-tissue fillers.
25828039	8	60	theme	ultrastructural	1121:1135	arg1	compositions					1137:1148	the ultrastructural compositions	1117:1148	the ultrastructural compositions	1117:1148	CONCLUSION Descriptions of the ultrastructural compositions and nature of BEL, JUV, and RES are helpful for matching the areas to be treated with the HA soft-tissue filler architecture.
25828039	8	61	theme	RES	1178:1180	arg1	nature					1154:1159	nature	1154:1159	nature of BEL, JUV, and RES	1154:1180	CONCLUSION Descriptions of the ultrastructural compositions and nature of BEL, JUV, and RES are helpful for matching the areas to be treated with the HA soft-tissue filler architecture.
25828039	8	61	theme	RES	1178:1180	arg1	Descriptions					1101:1112	CONCLUSION Descriptions	1090:1112	CONCLUSION Descriptions of the ultrastructural compositions	1090:1148	CONCLUSION Descriptions of the ultrastructural compositions and nature of BEL, JUV, and RES are helpful for matching the areas to be treated with the HA soft-tissue filler architecture.
25458304	4	0	theme	I	663:663	arg1	arabinogalactan					665:679	A type I arabinogalactan	656:679	A type I arabinogalactan	656:679	A type I arabinogalactan was purified via Fehling precipitation and ultrafiltration through 50 kDa (cut-off) membrane.
25458304	1	1	theme	fruit	249:253	arg1	pulp					214:217	ripe pulp	209:217	ripe pulp of the edible exotic tropical fruit named tamarillo (Solanum betaceum)	209:288	A structural characterization of polysaccharides obtained by aqueous extraction of ripe pulp of the edible exotic tropical fruit named tamarillo (Solanum betaceum) was carried out.
25458304	2	2	theme	α-amylase	346:354	arg1	treatments					356:365	freeze-thaw and α-amylase treatments	330:365	freeze-thaw and α-amylase treatments	330:365	After fractionation by freeze-thaw and α-amylase treatments, a fraction containing a mixture of highly-methoxylated homogalacturonan and of arabinogalactan was obtained.
25458304	0	3	from	Structure	0:8	arg1	tamarillo					63:71	the edible tropical fruit tamarillo	37:71	the edible tropical fruit tamarillo (Solanum betaceum)	37:90	Structure of an arabinogalactan from the edible tropical fruit tamarillo (Solanum betaceum) and its antinociceptive activity.
25458304	3	4	theme	%	520:520	arg1	DE					511:512	DE	511:512	DE	511:512	A degree of methylesterification (DE) of 71% and a degree of acetylation (DA) of 1.3% was determined by (1)H NMR spectroscopy and spectrophotometric quantification, respectively.
25458304	3	4	theme	%	520:520	arg1	methylesterification					489:508	methylesterification	489:508	methylesterification (DE) of 71%	489:520	A degree of methylesterification (DE) of 71% and a degree of acetylation (DA) of 1.3% was determined by (1)H NMR spectroscopy and spectrophotometric quantification, respectively.
25458304	4	5	theme	50	748:749	arg1	kDa					751:753	kDa	751:753	kDa	751:753	A type I arabinogalactan was purified via Fehling precipitation and ultrafiltration through 50 kDa (cut-off) membrane.
25458304	3	6	theme	acetylation	538:548	arg1	degree					479:484	A degree	477:484	A degree of methylesterification (DE) of 71%	477:520	A degree of methylesterification (DE) of 71% and a degree of acetylation (DA) of 1.3% was determined by (1)H NMR spectroscopy and spectrophotometric quantification, respectively.
25458304	3	6	theme	acetylation	538:548	arg1	degree					528:533	a degree	526:533	a degree of acetylation (DA) of 1.3%	526:561	A degree of methylesterification (DE) of 71% and a degree of acetylation (DA) of 1.3% was determined by (1)H NMR spectroscopy and spectrophotometric quantification, respectively.
25458304	2	7	theme	arabinogalactan	447:461	arg1	mixture					392:398	a mixture	390:398	a mixture of highly-methoxylated homogalacturonan and of arabinogalactan	390:461	After fractionation by freeze-thaw and α-amylase treatments, a fraction containing a mixture of highly-methoxylated homogalacturonan and of arabinogalactan was obtained.
25458304	5	8	theme	sugar	815:819	arg1	composition					821:831	sugar composition	815:831	sugar composition	815:831	Its chemical structure was performed by sugar composition, HPSEC, methylation, carboxy-reduction and (13)C NMR spectroscopy analysis.
25458304	4	9	theme	type	658:661	arg1	arabinogalactan					665:679	A type I arabinogalactan	656:679	A type I arabinogalactan	656:679	A type I arabinogalactan was purified via Fehling precipitation and ultrafiltration through 50 kDa (cut-off) membrane.
25458304	2	10	theme	homogalacturonan	423:438	arg1	mixture					392:398	a mixture	390:398	a mixture of highly-methoxylated homogalacturonan and of arabinogalactan	390:461	After fractionation by freeze-thaw and α-amylase treatments, a fraction containing a mixture of highly-methoxylated homogalacturonan and of arabinogalactan was obtained.
25458304	5	11	theme	C	880:880	arg1	spectroscopy					886:897	(13)C NMR spectroscopy	876:897	(13)C NMR spectroscopy	876:897	Its chemical structure was performed by sugar composition, HPSEC, methylation, carboxy-reduction and (13)C NMR spectroscopy analysis.
25458304	6	12	theme	2.5	1031:1033	arg1	%					1034:1034	%	1034:1034	%	1034:1034	Intraperitoneal administration of the arabinogalactan did not reduce the nociception induced by intraplantar injection of 2.5% formalin in mice, but significantly reduced the number of abdominal constrictions induced by 0.6% acetic acid, indicating that fraction has an antinociceptive effect on the visceral inflammatory pain model.
25458304	2	13	theme	highly-methoxylated	403:421	arg1	homogalacturonan					423:438	highly-methoxylated homogalacturonan	403:438	highly-methoxylated homogalacturonan	403:438	After fractionation by freeze-thaw and α-amylase treatments, a fraction containing a mixture of highly-methoxylated homogalacturonan and of arabinogalactan was obtained.
25458304	6	14	theme	pain	1231:1234	arg1	model					1236:1240	the visceral inflammatory pain model	1205:1240	the visceral inflammatory pain model	1205:1240	Intraperitoneal administration of the arabinogalactan did not reduce the nociception induced by intraplantar injection of 2.5% formalin in mice, but significantly reduced the number of abdominal constrictions induced by 0.6% acetic acid, indicating that fraction has an antinociceptive effect on the visceral inflammatory pain model.
25458304	4	15	theme	kDa	751:753	arg1	membrane					765:772	50 kDa (cut-off) membrane	748:772	50 kDa (cut-off) membrane	748:772	A type I arabinogalactan was purified via Fehling precipitation and ultrafiltration through 50 kDa (cut-off) membrane.
25458304	6	16	from	mice	1048:1051	arg1	injection					1018:1026	intraplantar injection	1005:1026	intraplantar injection of 2.5% formalin in mice	1005:1051	Intraperitoneal administration of the arabinogalactan did not reduce the nociception induced by intraplantar injection of 2.5% formalin in mice, but significantly reduced the number of abdominal constrictions induced by 0.6% acetic acid, indicating that fraction has an antinociceptive effect on the visceral inflammatory pain model.
25458304	6	17	theme	inflammatory	1218:1229	arg1	pain					1231:1234	the visceral inflammatory pain	1205:1234	the visceral inflammatory pain model	1205:1240	Intraperitoneal administration of the arabinogalactan did not reduce the nociception induced by intraplantar injection of 2.5% formalin in mice, but significantly reduced the number of abdominal constrictions induced by 0.6% acetic acid, indicating that fraction has an antinociceptive effect on the visceral inflammatory pain model.
25458304	6	18	from	injection	1018:1026	arg1	mice					1048:1051	mice	1048:1051	mice	1048:1051	Intraperitoneal administration of the arabinogalactan did not reduce the nociception induced by intraplantar injection of 2.5% formalin in mice, but significantly reduced the number of abdominal constrictions induced by 0.6% acetic acid, indicating that fraction has an antinociceptive effect on the visceral inflammatory pain model.
25458304	1	19	theme	aqueous	187:193	arg1	extraction					195:204	aqueous extraction	187:204	aqueous extraction of ripe pulp of the edible exotic tropical fruit named tamarillo (Solanum betaceum)	187:288	A structural characterization of polysaccharides obtained by aqueous extraction of ripe pulp of the edible exotic tropical fruit named tamarillo (Solanum betaceum) was carried out.
25458304	5	20	theme	chemical	779:786	arg1	structure					788:796	Its chemical structure	775:796	Its chemical structure	775:796	Its chemical structure was performed by sugar composition, HPSEC, methylation, carboxy-reduction and (13)C NMR spectroscopy analysis.
25458304	6	21	theme	arabinogalactan	947:961	arg1	administration					925:938	Intraperitoneal administration	909:938	Intraperitoneal administration of the arabinogalactan	909:961	Intraperitoneal administration of the arabinogalactan did not reduce the nociception induced by intraplantar injection of 2.5% formalin in mice, but significantly reduced the number of abdominal constrictions induced by 0.6% acetic acid, indicating that fraction has an antinociceptive effect on the visceral inflammatory pain model.
25458304	6	22	theme	visceral	1209:1216	arg1	pain					1231:1234	the visceral inflammatory pain	1205:1234	the visceral inflammatory pain model	1205:1240	Intraperitoneal administration of the arabinogalactan did not reduce the nociception induced by intraplantar injection of 2.5% formalin in mice, but significantly reduced the number of abdominal constrictions induced by 0.6% acetic acid, indicating that fraction has an antinociceptive effect on the visceral inflammatory pain model.
25458304	4	23	theme	Fehling	698:704	arg1	precipitation					706:718	Fehling precipitation	698:718	Fehling precipitation	698:718	A type I arabinogalactan was purified via Fehling precipitation and ultrafiltration through 50 kDa (cut-off) membrane.
25458304	6	24	theme	formalin	1036:1043	arg1	injection					1018:1026	intraplantar injection	1005:1026	intraplantar injection of 2.5% formalin in mice	1005:1051	Intraperitoneal administration of the arabinogalactan did not reduce the nociception induced by intraplantar injection of 2.5% formalin in mice, but significantly reduced the number of abdominal constrictions induced by 0.6% acetic acid, indicating that fraction has an antinociceptive effect on the visceral inflammatory pain model.
25458304	0	25	theme	arabinogalactan	16:30	arg1	Structure					0:8	Structure	0:8	Structure of an arabinogalactan from the edible tropical fruit tamarillo (Solanum betaceum)	0:90	Structure of an arabinogalactan from the edible tropical fruit tamarillo (Solanum betaceum) and its antinociceptive activity.
25458304	0	25	theme	arabinogalactan	16:30	arg1	activity					116:123	its antinociceptive activity	96:123	its antinociceptive activity	96:123	Structure of an arabinogalactan from the edible tropical fruit tamarillo (Solanum betaceum) and its antinociceptive activity.
25458304	6	26	theme	antinociceptive	1179:1193	arg1	effect					1195:1200	an antinociceptive effect	1176:1200	an antinociceptive effect	1176:1200	Intraperitoneal administration of the arabinogalactan did not reduce the nociception induced by intraplantar injection of 2.5% formalin in mice, but significantly reduced the number of abdominal constrictions induced by 0.6% acetic acid, indicating that fraction has an antinociceptive effect on the visceral inflammatory pain model.
25458304	0	27	dep	tamarillo	63:71	arg1	betaceum					82:89	Solanum betaceum	74:89	Solanum betaceum	74:89	Structure of an arabinogalactan from the edible tropical fruit tamarillo (Solanum betaceum) and its antinociceptive activity.
25458304	6	28	theme	%	1034:1034	arg1	formalin					1036:1043	2.5% formalin	1031:1043	2.5% formalin in mice	1031:1051	Intraperitoneal administration of the arabinogalactan did not reduce the nociception induced by intraplantar injection of 2.5% formalin in mice, but significantly reduced the number of abdominal constrictions induced by 0.6% acetic acid, indicating that fraction has an antinociceptive effect on the visceral inflammatory pain model.
25458304	0	29	theme	antinociceptive	100:114	arg1	activity					116:123	its antinociceptive activity	96:123	its antinociceptive activity	96:123	Structure of an arabinogalactan from the edible tropical fruit tamarillo (Solanum betaceum) and its antinociceptive activity.
25458304	1	30	theme	polysaccharides	159:173	arg1	characterization					139:154	A structural characterization	126:154	A structural characterization of polysaccharides obtained by aqueous extraction of ripe pulp of the edible exotic tropical fruit named tamarillo (Solanum betaceum)	126:288	A structural characterization of polysaccharides obtained by aqueous extraction of ripe pulp of the edible exotic tropical fruit named tamarillo (Solanum betaceum) was carried out.
25458304	3	31	theme	H	584:584	arg1	spectroscopy					590:601	(1)H NMR spectroscopy	581:601	(1)H NMR spectroscopy	581:601	A degree of methylesterification (DE) of 71% and a degree of acetylation (DA) of 1.3% was determined by (1)H NMR spectroscopy and spectrophotometric quantification, respectively.
25458304	1	32	theme	ripe	209:212	arg1	pulp					214:217	ripe pulp	209:217	ripe pulp of the edible exotic tropical fruit named tamarillo (Solanum betaceum)	209:288	A structural characterization of polysaccharides obtained by aqueous extraction of ripe pulp of the edible exotic tropical fruit named tamarillo (Solanum betaceum) was carried out.
25458304	6	33	theme	abdominal	1094:1102	arg1	constrictions					1104:1116	abdominal constrictions	1094:1116	abdominal constrictions induced by 0.6% acetic acid	1094:1144	Intraperitoneal administration of the arabinogalactan did not reduce the nociception induced by intraplantar injection of 2.5% formalin in mice, but significantly reduced the number of abdominal constrictions induced by 0.6% acetic acid, indicating that fraction has an antinociceptive effect on the visceral inflammatory pain model.
25458304	2	34	contain	containing	379:388	arg1	fraction					370:377	a fraction	368:377	a fraction containing a mixture of highly-methoxylated homogalacturonan and of arabinogalactan	368:461	After fractionation by freeze-thaw and α-amylase treatments, a fraction containing a mixture of highly-methoxylated homogalacturonan and of arabinogalactan was obtained.
25458304	2	34	contain	containing	379:388	arg2	mixture					392:398	a mixture	390:398	a mixture of highly-methoxylated homogalacturonan and of arabinogalactan	390:461	After fractionation by freeze-thaw and α-amylase treatments, a fraction containing a mixture of highly-methoxylated homogalacturonan and of arabinogalactan was obtained.
25458304	1	35	theme	pulp	214:217	arg1	extraction					195:204	aqueous extraction	187:204	aqueous extraction of ripe pulp of the edible exotic tropical fruit named tamarillo (Solanum betaceum)	187:288	A structural characterization of polysaccharides obtained by aqueous extraction of ripe pulp of the edible exotic tropical fruit named tamarillo (Solanum betaceum) was carried out.
25458304	6	36	theme	Intraperitoneal	909:923	arg1	administration					925:938	Intraperitoneal administration	909:938	Intraperitoneal administration of the arabinogalactan	909:961	Intraperitoneal administration of the arabinogalactan did not reduce the nociception induced by intraplantar injection of 2.5% formalin in mice, but significantly reduced the number of abdominal constrictions induced by 0.6% acetic acid, indicating that fraction has an antinociceptive effect on the visceral inflammatory pain model.
25458304	0	37	from	tamarillo	63:71	arg1	Structure					0:8	Structure	0:8	Structure of an arabinogalactan from the edible tropical fruit tamarillo (Solanum betaceum)	0:90	Structure of an arabinogalactan from the edible tropical fruit tamarillo (Solanum betaceum) and its antinociceptive activity.
25458304	0	37	from	tamarillo	63:71	arg1	arabinogalactan					16:30	an arabinogalactan	13:30	an arabinogalactan from the edible tropical fruit tamarillo (Solanum betaceum)	13:90	Structure of an arabinogalactan from the edible tropical fruit tamarillo (Solanum betaceum) and its antinociceptive activity.
25458304	0	37	from	tamarillo	63:71	arg1	activity					116:123	its antinociceptive activity	96:123	its antinociceptive activity	96:123	Structure of an arabinogalactan from the edible tropical fruit tamarillo (Solanum betaceum) and its antinociceptive activity.
25458304	0	38	from	activity	116:123	arg1	tamarillo					63:71	the edible tropical fruit tamarillo	37:71	the edible tropical fruit tamarillo (Solanum betaceum)	37:90	Structure of an arabinogalactan from the edible tropical fruit tamarillo (Solanum betaceum) and its antinociceptive activity.
25458304	0	39	theme	tropical	48:55	arg1	tamarillo					63:71	the edible tropical fruit tamarillo	37:71	the edible tropical fruit tamarillo (Solanum betaceum)	37:90	Structure of an arabinogalactan from the edible tropical fruit tamarillo (Solanum betaceum) and its antinociceptive activity.
25458304	3	40	theme	NMR	586:588	arg1	spectroscopy					590:601	(1)H NMR spectroscopy	581:601	(1)H NMR spectroscopy	581:601	A degree of methylesterification (DE) of 71% and a degree of acetylation (DA) of 1.3% was determined by (1)H NMR spectroscopy and spectrophotometric quantification, respectively.
25458304	6	41	theme	constrictions	1104:1116	arg1	number					1084:1089	the number	1080:1089	the number of abdominal constrictions induced by 0.6% acetic acid	1080:1144	Intraperitoneal administration of the arabinogalactan did not reduce the nociception induced by intraplantar injection of 2.5% formalin in mice, but significantly reduced the number of abdominal constrictions induced by 0.6% acetic acid, indicating that fraction has an antinociceptive effect on the visceral inflammatory pain model.
25458304	0	42	theme	edible	41:46	arg1	tamarillo					63:71	the edible tropical fruit tamarillo	37:71	the edible tropical fruit tamarillo (Solanum betaceum)	37:90	Structure of an arabinogalactan from the edible tropical fruit tamarillo (Solanum betaceum) and its antinociceptive activity.
25458304	6	43	contain	has	1172:1174	arg2	effect					1195:1200	an antinociceptive effect	1176:1200	an antinociceptive effect	1176:1200	Intraperitoneal administration of the arabinogalactan did not reduce the nociception induced by intraplantar injection of 2.5% formalin in mice, but significantly reduced the number of abdominal constrictions induced by 0.6% acetic acid, indicating that fraction has an antinociceptive effect on the visceral inflammatory pain model.
25458304	6	43	contain	has	1172:1174	arg1	fraction					1163:1170	fraction	1163:1170	fraction	1163:1170	Intraperitoneal administration of the arabinogalactan did not reduce the nociception induced by intraplantar injection of 2.5% formalin in mice, but significantly reduced the number of abdominal constrictions induced by 0.6% acetic acid, indicating that fraction has an antinociceptive effect on the visceral inflammatory pain model.
25458304	1	44	dep	tamarillo	261:269	arg1	betaceum					280:287	Solanum betaceum	272:287	Solanum betaceum	272:287	A structural characterization of polysaccharides obtained by aqueous extraction of ripe pulp of the edible exotic tropical fruit named tamarillo (Solanum betaceum) was carried out.
25458304	3	45	theme	%	561:561	arg1	DA					551:552	DA	551:552	DA	551:552	A degree of methylesterification (DE) of 71% and a degree of acetylation (DA) of 1.3% was determined by (1)H NMR spectroscopy and spectrophotometric quantification, respectively.
25458304	3	45	theme	%	561:561	arg1	acetylation					538:548	acetylation	538:548	acetylation (DA) of 1.3%	538:561	A degree of methylesterification (DE) of 71% and a degree of acetylation (DA) of 1.3% was determined by (1)H NMR spectroscopy and spectrophotometric quantification, respectively.
25458304	2	46	theme	freeze-thaw	330:340	arg1	treatments					356:365	freeze-thaw and α-amylase treatments	330:365	freeze-thaw and α-amylase treatments	330:365	After fractionation by freeze-thaw and α-amylase treatments, a fraction containing a mixture of highly-methoxylated homogalacturonan and of arabinogalactan was obtained.
25458304	6	47	from	formalin	1036:1043	arg1	mice					1048:1051	mice	1048:1051	mice	1048:1051	Intraperitoneal administration of the arabinogalactan did not reduce the nociception induced by intraplantar injection of 2.5% formalin in mice, but significantly reduced the number of abdominal constrictions induced by 0.6% acetic acid, indicating that fraction has an antinociceptive effect on the visceral inflammatory pain model.
25458304	3	48	theme	methylesterification	489:508	arg1	degree					479:484	A degree	477:484	A degree of methylesterification (DE) of 71%	477:520	A degree of methylesterification (DE) of 71% and a degree of acetylation (DA) of 1.3% was determined by (1)H NMR spectroscopy and spectrophotometric quantification, respectively.
25458304	3	48	theme	methylesterification	489:508	arg1	degree					528:533	a degree	526:533	a degree of acetylation (DA) of 1.3%	526:561	A degree of methylesterification (DE) of 71% and a degree of acetylation (DA) of 1.3% was determined by (1)H NMR spectroscopy and spectrophotometric quantification, respectively.
25458304	6	49	theme	intraplantar	1005:1016	arg1	injection					1018:1026	intraplantar injection	1005:1026	intraplantar injection of 2.5% formalin in mice	1005:1051	Intraperitoneal administration of the arabinogalactan did not reduce the nociception induced by intraplantar injection of 2.5% formalin in mice, but significantly reduced the number of abdominal constrictions induced by 0.6% acetic acid, indicating that fraction has an antinociceptive effect on the visceral inflammatory pain model.
25458304	6	50	theme	acetic	1134:1139	arg1	acid					1141:1144	0.6% acetic acid	1129:1144	0.6% acetic acid	1129:1144	Intraperitoneal administration of the arabinogalactan did not reduce the nociception induced by intraplantar injection of 2.5% formalin in mice, but significantly reduced the number of abdominal constrictions induced by 0.6% acetic acid, indicating that fraction has an antinociceptive effect on the visceral inflammatory pain model.
25458304	3	51	theme	spectrophotometric	607:624	arg1	quantification					626:639	spectrophotometric quantification	607:639	spectrophotometric quantification	607:639	A degree of methylesterification (DE) of 71% and a degree of acetylation (DA) of 1.3% was determined by (1)H NMR spectroscopy and spectrophotometric quantification, respectively.
25458304	5	52	dep	composition	821:831	arg1	analysis					899:906	analysis	899:906	analysis	899:906	Its chemical structure was performed by sugar composition, HPSEC, methylation, carboxy-reduction and (13)C NMR spectroscopy analysis.
25458304	5	53	theme	NMR	882:884	arg1	spectroscopy					886:897	(13)C NMR spectroscopy	876:897	(13)C NMR spectroscopy	876:897	Its chemical structure was performed by sugar composition, HPSEC, methylation, carboxy-reduction and (13)C NMR spectroscopy analysis.
25458304	1	54	theme	edible	226:231	arg1	fruit					249:253	the edible exotic tropical fruit	222:253	the edible exotic tropical fruit named tamarillo (Solanum betaceum)	222:288	A structural characterization of polysaccharides obtained by aqueous extraction of ripe pulp of the edible exotic tropical fruit named tamarillo (Solanum betaceum) was carried out.
25458304	1	55	theme	structural	128:137	arg1	characterization					139:154	A structural characterization	126:154	A structural characterization of polysaccharides obtained by aqueous extraction of ripe pulp of the edible exotic tropical fruit named tamarillo (Solanum betaceum)	126:288	A structural characterization of polysaccharides obtained by aqueous extraction of ripe pulp of the edible exotic tropical fruit named tamarillo (Solanum betaceum) was carried out.
25458304	1	56	theme	exotic	233:238	arg1	fruit					249:253	the edible exotic tropical fruit	222:253	the edible exotic tropical fruit named tamarillo (Solanum betaceum)	222:288	A structural characterization of polysaccharides obtained by aqueous extraction of ripe pulp of the edible exotic tropical fruit named tamarillo (Solanum betaceum) was carried out.
25458304	0	57	theme	fruit	57:61	arg1	tamarillo					63:71	the edible tropical fruit tamarillo	37:71	the edible tropical fruit tamarillo (Solanum betaceum)	37:90	Structure of an arabinogalactan from the edible tropical fruit tamarillo (Solanum betaceum) and its antinociceptive activity.
25458304	4	58	dep	kDa	751:753	arg1	cut-off					756:762	cut-off	756:762	cut-off	756:762	A type I arabinogalactan was purified via Fehling precipitation and ultrafiltration through 50 kDa (cut-off) membrane.
25458304	1	59	theme	tropical	240:247	arg1	fruit					249:253	the edible exotic tropical fruit	222:253	the edible exotic tropical fruit named tamarillo (Solanum betaceum)	222:288	A structural characterization of polysaccharides obtained by aqueous extraction of ripe pulp of the edible exotic tropical fruit named tamarillo (Solanum betaceum) was carried out.
27019242	3	0	theme	higher	380:385	arg1	decrease					387:394	A higher decrease	378:394	A higher decrease of energy intake (-383 kcal/day)	378:427	A higher decrease of energy intake (-383 kcal/day) was also found in women who ate whey supplements, while in the casein and control group the decrease was only -144 and -70 kcal/day respectively.
27019242	1	1	theme	women	184:188	arg1	satiety					159:165	satiety	159:165	satiety	159:165	It has been studied the effect of three kinds of supplements (whey, casein and maltodextrin, as control) in the regulation of food intake and satiety of 60 overweight women.
27019242	1	1	theme	women	184:188	arg1	intake					148:153	food intake	143:153	food intake	143:153	It has been studied the effect of three kinds of supplements (whey, casein and maltodextrin, as control) in the regulation of food intake and satiety of 60 overweight women.
27019242	2	2	theme	control	362:368	arg1	groups					370:375	control groups	362:375	control groups	362:375	After 10 weeks, significant differences (p < 0.001) were found with regard to reduction of weight, IMC, % fat and waist circumference in the whey group against casein and control groups.
27019242	1	3	from	effect	41:46	arg1	regulation					129:138	the regulation	125:138	the regulation of food intake and satiety of 60 overweight women	125:188	It has been studied the effect of three kinds of supplements (whey, casein and maltodextrin, as control) in the regulation of food intake and satiety of 60 overweight women.
27019242	2	4	theme	significant	207:217	arg1	p					232:232	p < 0.001	232:240	p < 0.001	232:240	After 10 weeks, significant differences (p < 0.001) were found with regard to reduction of weight, IMC, % fat and waist circumference in the whey group against casein and control groups.
27019242	2	4	theme	significant	207:217	arg1	differences					219:229	significant differences	207:229	significant differences (p < 0.001)	207:241	After 10 weeks, significant differences (p < 0.001) were found with regard to reduction of weight, IMC, % fat and waist circumference in the whey group against casein and control groups.
27019242	3	5	located	found	438:442	arg2	decrease					387:394	A higher decrease	378:394	A higher decrease of energy intake (-383 kcal/day)	378:427	A higher decrease of energy intake (-383 kcal/day) was also found in women who ate whey supplements, while in the casein and control group the decrease was only -144 and -70 kcal/day respectively.
27019242	3	5	located	found	438:442	arg1	women					447:451	women	447:451	women who ate whey supplements	447:476	A higher decrease of energy intake (-383 kcal/day) was also found in women who ate whey supplements, while in the casein and control group the decrease was only -144 and -70 kcal/day respectively.
27019242	3	6	theme	intake	406:411	arg1	decrease					387:394	A higher decrease	378:394	A higher decrease of energy intake (-383 kcal/day)	378:427	A higher decrease of energy intake (-383 kcal/day) was also found in women who ate whey supplements, while in the casein and control group the decrease was only -144 and -70 kcal/day respectively.
27019242	2	7	theme	IMC	290:292	arg1	reduction					269:277	reduction	269:277	reduction of weight, IMC, % fat and waist circumference in the whey group against casein and control groups	269:375	After 10 weeks, significant differences (p < 0.001) were found with regard to reduction of weight, IMC, % fat and waist circumference in the whey group against casein and control groups.
27019242	2	8	theme	whey	332:335	arg1	group					337:341	the whey group	328:341	the whey group	328:341	After 10 weeks, significant differences (p < 0.001) were found with regard to reduction of weight, IMC, % fat and waist circumference in the whey group against casein and control groups.
27019242	1	9	theme	kinds	57:61	arg1	effect					41:46	the effect	37:46	the effect of three kinds of supplements (whey, casein and maltodextrin, as control) in the regulation of food intake and satiety of 60 overweight women	37:188	It has been studied the effect of three kinds of supplements (whey, casein and maltodextrin, as control) in the regulation of food intake and satiety of 60 overweight women.
27019242	2	10	theme	<	234:234	arg1	p					232:232	p < 0.001	232:240	p < 0.001	232:240	After 10 weeks, significant differences (p < 0.001) were found with regard to reduction of weight, IMC, % fat and waist circumference in the whey group against casein and control groups.
27019242	2	10	theme	<	234:234	arg1	differences					219:229	significant differences	207:229	significant differences (p < 0.001)	207:241	After 10 weeks, significant differences (p < 0.001) were found with regard to reduction of weight, IMC, % fat and waist circumference in the whey group against casein and control groups.
27019242	2	11	theme	weight	282:287	arg1	reduction					269:277	reduction	269:277	reduction of weight, IMC, % fat and waist circumference in the whey group against casein and control groups	269:375	After 10 weeks, significant differences (p < 0.001) were found with regard to reduction of weight, IMC, % fat and waist circumference in the whey group against casein and control groups.
27019242	2	12	from	reduction	269:277	arg1	group					337:341	the whey group	328:341	the whey group	328:341	After 10 weeks, significant differences (p < 0.001) were found with regard to reduction of weight, IMC, % fat and waist circumference in the whey group against casein and control groups.
27019242	1	13	theme	supplements	66:76	arg1	kinds					57:61	three kinds	51:61	three kinds of supplements (whey, casein and maltodextrin, as control)	51:120	It has been studied the effect of three kinds of supplements (whey, casein and maltodextrin, as control) in the regulation of food intake and satiety of 60 overweight women.
27019242	2	14	theme	waist	305:309	arg1	circumference					311:323	waist circumference	305:323	waist circumference	305:323	After 10 weeks, significant differences (p < 0.001) were found with regard to reduction of weight, IMC, % fat and waist circumference in the whey group against casein and control groups.
27019242	2	15	theme	circumference	311:323	arg1	reduction					269:277	reduction	269:277	reduction of weight, IMC, % fat and waist circumference in the whey group against casein and control groups	269:375	After 10 weeks, significant differences (p < 0.001) were found with regard to reduction of weight, IMC, % fat and waist circumference in the whey group against casein and control groups.
27019242	3	16	theme	casein	492:497	arg1	group					511:515	the casein and control group	488:515	group	511:515	A higher decrease of energy intake (-383 kcal/day) was also found in women who ate whey supplements, while in the casein and control group the decrease was only -144 and -70 kcal/day respectively.
27019242	4	17	theme	satiety	584:590	arg1	effect					592:597	satiety effect	584:597	satiety effect	584:597	Finally, satiety effect was more efficiently promoted by whey against casein and maltodextrins.
27019242	1	18	theme	food	143:146	arg1	intake					148:153	food intake	143:153	food intake	143:153	It has been studied the effect of three kinds of supplements (whey, casein and maltodextrin, as control) in the regulation of food intake and satiety of 60 overweight women.
27019242	1	19	theme	intake	148:153	arg1	regulation					129:138	the regulation	125:138	the regulation of food intake and satiety of 60 overweight women	125:188	It has been studied the effect of three kinds of supplements (whey, casein and maltodextrin, as control) in the regulation of food intake and satiety of 60 overweight women.
27019242	2	20	theme	fat	297:299	arg1	reduction					269:277	reduction	269:277	reduction of weight, IMC, % fat and waist circumference in the whey group against casein and control groups	269:375	After 10 weeks, significant differences (p < 0.001) were found with regard to reduction of weight, IMC, % fat and waist circumference in the whey group against casein and control groups.
27019242	2	21	theme	%	295:295	arg1	fat					297:299	% fat	295:299	% fat	295:299	After 10 weeks, significant differences (p < 0.001) were found with regard to reduction of weight, IMC, % fat and waist circumference in the whey group against casein and control groups.
27019242	3	22	from	-144	539:542	arg1	group					511:515	the casein and control group	488:515	group	511:515	A higher decrease of energy intake (-383 kcal/day) was also found in women who ate whey supplements, while in the casein and control group the decrease was only -144 and -70 kcal/day respectively.
27019242	1	23	theme	satiety	159:165	arg1	regulation					129:138	the regulation	125:138	the regulation of food intake and satiety of 60 overweight women	125:188	It has been studied the effect of three kinds of supplements (whey, casein and maltodextrin, as control) in the regulation of food intake and satiety of 60 overweight women.
27019242	3	24	theme	control	503:509	arg1	group					511:515	the casein and control group	488:515	group	511:515	A higher decrease of energy intake (-383 kcal/day) was also found in women who ate whey supplements, while in the casein and control group the decrease was only -144 and -70 kcal/day respectively.
27019242	3	25	theme	whey	461:464	arg1	supplements					466:476	whey supplements	461:476	whey supplements	461:476	A higher decrease of energy intake (-383 kcal/day) was also found in women who ate whey supplements, while in the casein and control group the decrease was only -144 and -70 kcal/day respectively.
27019242	1	26	dep	supplements	66:76	arg1	casein					85:90	casein	85:90	casein	85:90	It has been studied the effect of three kinds of supplements (whey, casein and maltodextrin, as control) in the regulation of food intake and satiety of 60 overweight women.
27019242	1	26	dep	supplements	66:76	arg1	whey					79:82	whey	79:82	whey	79:82	It has been studied the effect of three kinds of supplements (whey, casein and maltodextrin, as control) in the regulation of food intake and satiety of 60 overweight women.
27019242	1	26	dep	supplements	66:76	arg1	supplements					66:76	supplements	66:76	supplements (whey, casein and maltodextrin, as control)	66:120	It has been studied the effect of three kinds of supplements (whey, casein and maltodextrin, as control) in the regulation of food intake and satiety of 60 overweight women.
27019242	1	26	dep	supplements	66:76	arg1	maltodextrin					96:107	maltodextrin	96:107	maltodextrin	96:107	It has been studied the effect of three kinds of supplements (whey, casein and maltodextrin, as control) in the regulation of food intake and satiety of 60 overweight women.
27019242	3	27	theme	energy	399:404	arg1	intake					406:411	energy intake	399:411	energy intake (-383 kcal/day)	399:427	A higher decrease of energy intake (-383 kcal/day) was also found in women who ate whey supplements, while in the casein and control group the decrease was only -144 and -70 kcal/day respectively.
27019242	3	27	theme	energy	399:404	arg1	kcal/day					419:426	-383 kcal/day	414:426	-383 kcal/day	414:426	A higher decrease of energy intake (-383 kcal/day) was also found in women who ate whey supplements, while in the casein and control group the decrease was only -144 and -70 kcal/day respectively.
27019242	1	28	theme	overweight	173:182	arg1	women					184:188	60 overweight women	170:188	60 overweight women	170:188	It has been studied the effect of three kinds of supplements (whey, casein and maltodextrin, as control) in the regulation of food intake and satiety of 60 overweight women.
26344283	9	0	theme	CS	926:927	arg1	film					929:932	CS film	926:932	CS film	926:932	The thermal stability of CS film showed no significant effect of CN addition, whereas contact angle measurements revealed that CN addition resulted in an increment of hydrophilicity of chitosan films.
26344283	7	1	theme	high	745:748	arg1	interaction					750:760	This high interaction	740:760	This high interaction	740:760	This high interaction led to an increment of the crystallinity of chitosan films.
26344283	9	2	theme	film	929:932	arg1	stability					913:921	The thermal stability	901:921	The thermal stability of CS film	901:932	The thermal stability of CS film showed no significant effect of CN addition, whereas contact angle measurements revealed that CN addition resulted in an increment of hydrophilicity of chitosan films.
26344283	4	3	theme	angle	504:508	arg1	measurements					510:521	contact angle measurements	496:521	contact angle measurements	496:521	The prepared nanocomposites were characterized by FTIR, XRD, SEM, DSC, TGA, TMA and contact angle measurements.
26344283	4	4	theme	prepared	416:423	arg1	nanocomposites					425:438	The prepared nanocomposites	412:438	The prepared nanocomposites	412:438	The prepared nanocomposites were characterized by FTIR, XRD, SEM, DSC, TGA, TMA and contact angle measurements.
26344283	4	5	theme	contact	496:502	arg1	measurements					510:521	contact angle measurements	496:521	contact angle measurements	496:521	The prepared nanocomposites were characterized by FTIR, XRD, SEM, DSC, TGA, TMA and contact angle measurements.
26344283	3	6	used	used	358:361	arg2	stirring/ultrasonication					307:330	stirring/ultrasonication	307:330	stirring/ultrasonication	307:330	Mechanical stirring/ultrasonication or microfluidization were used to disperse nanocrystals in the chitosan matrix.
26344283	3	6	used	used	358:361	arg2	microfluidization					335:351	microfluidization	335:351	microfluidization	335:351	Mechanical stirring/ultrasonication or microfluidization were used to disperse nanocrystals in the chitosan matrix.
26344283	6	7	theme	bonds	626:630	arg1	Formation					604:612	Formation	604:612	Formation of hydrogen bonds between CS and CN in nanocomposites prepared by using microfluidization	604:702	Formation of hydrogen bonds between CS and CN in nanocomposites prepared by using microfluidization was confirmed by FTIR spectroscopy.
26344283	8	8	theme	Tg	822:823	arg1	values					825:830	Tg values	822:830	Tg values within a range of 53-58°C	822:856	Tg values within a range of 53-58°C were obtained in DSC and TMA measurements.
26344283	2	9	theme	composites	266:275	arg1	properties					252:261	the properties	248:261	the properties of composites	248:275	Influences of solution preparation method and CN content on the properties of composites were investigated.
26344283	9	10	theme	significant	944:954	arg1	effect					956:961	no significant effect	941:961	no significant effect of CN addition	941:976	The thermal stability of CS film showed no significant effect of CN addition, whereas contact angle measurements revealed that CN addition resulted in an increment of hydrophilicity of chitosan films.
26344283	9	11	theme	hydrophilicity	1068:1081	arg1	increment					1055:1063	an increment	1052:1063	an increment of hydrophilicity of chitosan films	1052:1099	The thermal stability of CS film showed no significant effect of CN addition, whereas contact angle measurements revealed that CN addition resulted in an increment of hydrophilicity of chitosan films.
26344283	6	12	theme	FTIR	721:724	arg1	spectroscopy					726:737	FTIR spectroscopy	721:737	FTIR spectroscopy	721:737	Formation of hydrogen bonds between CS and CN in nanocomposites prepared by using microfluidization was confirmed by FTIR spectroscopy.
26344283	2	13	from	Influences	188:197	arg1	properties					252:261	the properties	248:261	the properties of composites	248:275	Influences of solution preparation method and CN content on the properties of composites were investigated.
26344283	5	14	theme	CN	579:580	arg1	aggregates					582:591	CN aggregates	579:591	CN aggregates	579:591	SEM analysis revealed that microfluidization decreased CN aggregates in matrix.
26344283	1	15	theme	Biocomposites	81:93	arg1	nanocrystals					122:133	Biocomposites of chitosan (CS)/cellulose nanocrystals	81:133	Biocomposites of chitosan (CS)/cellulose nanocrystals (CN)	81:138	Biocomposites of chitosan (CS)/cellulose nanocrystals (CN) were prepared by using solution casting method.
26344283	1	15	theme	Biocomposites	81:93	arg1	CN					136:137	CN	136:137	CN	136:137	Biocomposites of chitosan (CS)/cellulose nanocrystals (CN) were prepared by using solution casting method.
26344283	8	16	theme	TMA	883:885	arg1	measurements					887:898	TMA measurements	883:898	TMA measurements	883:898	Tg values within a range of 53-58°C were obtained in DSC and TMA measurements.
26344283	6	17	theme	hydrogen	617:624	arg1	bonds					626:630	hydrogen bonds	617:630	hydrogen bonds	617:630	Formation of hydrogen bonds between CS and CN in nanocomposites prepared by using microfluidization was confirmed by FTIR spectroscopy.
26344283	9	18	theme	chitosan	1086:1093	arg1	films					1095:1099	chitosan films	1086:1099	chitosan films	1086:1099	The thermal stability of CS film showed no significant effect of CN addition, whereas contact angle measurements revealed that CN addition resulted in an increment of hydrophilicity of chitosan films.
26344283	1	19	theme	solution	163:170	arg1	method					180:185	solution casting method	163:185	solution casting method	163:185	Biocomposites of chitosan (CS)/cellulose nanocrystals (CN) were prepared by using solution casting method.
26344283	9	20	theme	CN	966:967	arg1	addition					969:976	CN addition	966:976	CN addition	966:976	The thermal stability of CS film showed no significant effect of CN addition, whereas contact angle measurements revealed that CN addition resulted in an increment of hydrophilicity of chitosan films.
26344283	1	21	theme	chitosan	98:105	arg1	Biocomposites					81:93	Biocomposites	81:93	Biocomposites of chitosan (CS)/cellulose nanocrystals (CN)	81:138	Biocomposites of chitosan (CS)/cellulose nanocrystals (CN) were prepared by using solution casting method.
26344283	1	21	theme	chitosan	98:105	arg1	CS					108:109	CS	108:109	CS	108:109	Biocomposites of chitosan (CS)/cellulose nanocrystals (CN) were prepared by using solution casting method.
26344283	9	22	theme	films	1095:1099	arg1	hydrophilicity					1068:1081	hydrophilicity	1068:1081	hydrophilicity of chitosan films	1068:1099	The thermal stability of CS film showed no significant effect of CN addition, whereas contact angle measurements revealed that CN addition resulted in an increment of hydrophilicity of chitosan films.
26344283	1	23	theme	casting	172:178	arg1	method					180:185	solution casting method	163:185	solution casting method	163:185	Biocomposites of chitosan (CS)/cellulose nanocrystals (CN) were prepared by using solution casting method.
26344283	9	24	theme	addition	969:976	arg1	effect					956:961	no significant effect	941:961	no significant effect of CN addition	941:976	The thermal stability of CS film showed no significant effect of CN addition, whereas contact angle measurements revealed that CN addition resulted in an increment of hydrophilicity of chitosan films.
26344283	3	25	theme	chitosan	395:402	arg1	matrix					404:409	the chitosan matrix	391:409	the chitosan matrix	391:409	Mechanical stirring/ultrasonication or microfluidization were used to disperse nanocrystals in the chitosan matrix.
26344283	7	26	theme	chitosan	806:813	arg1	films					815:819	chitosan films	806:819	chitosan films	806:819	This high interaction led to an increment of the crystallinity of chitosan films.
26344283	7	27	theme	films	815:819	arg1	crystallinity					789:801	the crystallinity	785:801	the crystallinity of chitosan films	785:819	This high interaction led to an increment of the crystallinity of chitosan films.
26344283	0	28	theme	processing	11:20	arg1	Effects					0:6	Effects	0:6	Effects of processing on the properties of chitosan/cellulose nanocrystal films	0:78	Effects of processing on the properties of chitosan/cellulose nanocrystal films.
26344283	7	29	theme	crystallinity	789:801	arg1	increment					772:780	an increment	769:780	an increment of the crystallinity of chitosan films	769:819	This high interaction led to an increment of the crystallinity of chitosan films.
26344283	9	30	theme	contact	987:993	arg1	measurements					1001:1012	contact angle measurements	987:1012	contact angle measurements	987:1012	The thermal stability of CS film showed no significant effect of CN addition, whereas contact angle measurements revealed that CN addition resulted in an increment of hydrophilicity of chitosan films.
26344283	3	31	from	nanocrystals	375:386	arg1	matrix					404:409	the chitosan matrix	391:409	the chitosan matrix	391:409	Mechanical stirring/ultrasonication or microfluidization were used to disperse nanocrystals in the chitosan matrix.
26344283	2	32	theme	method	223:228	arg1	Influences					188:197	Influences	188:197	Influences of solution preparation method and CN content on the properties of composites	188:275	Influences of solution preparation method and CN content on the properties of composites were investigated.
26344283	0	33	theme	chitosan/cellulose	43:60	arg1	films					74:78	chitosan/cellulose nanocrystal films	43:78	chitosan/cellulose nanocrystal films	43:78	Effects of processing on the properties of chitosan/cellulose nanocrystal films.
26344283	3	34	theme	Mechanical	296:305	arg1	stirring/ultrasonication					307:330	stirring/ultrasonication	307:330	stirring/ultrasonication	307:330	Mechanical stirring/ultrasonication or microfluidization were used to disperse nanocrystals in the chitosan matrix.
26344283	2	35	theme	preparation	211:221	arg1	method					223:228	solution preparation method	202:228	solution preparation method	202:228	Influences of solution preparation method and CN content on the properties of composites were investigated.
26344283	0	36	from	Effects	0:6	arg1	properties					29:38	the properties	25:38	the properties of chitosan/cellulose nanocrystal films	25:78	Effects of processing on the properties of chitosan/cellulose nanocrystal films.
26344283	2	37	theme	solution	202:209	arg1	method					223:228	solution preparation method	202:228	solution preparation method	202:228	Influences of solution preparation method and CN content on the properties of composites were investigated.
26344283	1	38	theme	/cellulose	111:120	arg1	nanocrystals					122:133	Biocomposites of chitosan (CS)/cellulose nanocrystals	81:133	Biocomposites of chitosan (CS)/cellulose nanocrystals (CN)	81:138	Biocomposites of chitosan (CS)/cellulose nanocrystals (CN) were prepared by using solution casting method.
26344283	1	38	theme	/cellulose	111:120	arg1	CN					136:137	CN	136:137	CN	136:137	Biocomposites of chitosan (CS)/cellulose nanocrystals (CN) were prepared by using solution casting method.
26344283	6	39	from	CS	640:641	arg1	nanocomposites					653:666	nanocomposites	653:666	nanocomposites prepared by using microfluidization	653:702	Formation of hydrogen bonds between CS and CN in nanocomposites prepared by using microfluidization was confirmed by FTIR spectroscopy.
26344283	9	40	dep	showed	934:939	arg1	whereas					979:985	whereas	979:985	whereas	979:985	The thermal stability of CS film showed no significant effect of CN addition, whereas contact angle measurements revealed that CN addition resulted in an increment of hydrophilicity of chitosan films.
26344283	6	41	from	CN	647:648	arg1	nanocomposites					653:666	nanocomposites	653:666	nanocomposites prepared by using microfluidization	653:702	Formation of hydrogen bonds between CS and CN in nanocomposites prepared by using microfluidization was confirmed by FTIR spectroscopy.
26344283	9	42	theme	thermal	905:911	arg1	stability					913:921	The thermal stability	901:921	The thermal stability of CS film	901:932	The thermal stability of CS film showed no significant effect of CN addition, whereas contact angle measurements revealed that CN addition resulted in an increment of hydrophilicity of chitosan films.
26344283	9	43	theme	CN	1028:1029	arg1	addition					1031:1038	CN addition	1028:1038	CN addition	1028:1038	The thermal stability of CS film showed no significant effect of CN addition, whereas contact angle measurements revealed that CN addition resulted in an increment of hydrophilicity of chitosan films.
26344283	0	44	theme	films	74:78	arg1	properties					29:38	the properties	25:38	the properties of chitosan/cellulose nanocrystal films	25:78	Effects of processing on the properties of chitosan/cellulose nanocrystal films.
26344283	2	45	theme	content	237:243	arg1	Influences					188:197	Influences	188:197	Influences of solution preparation method and CN content on the properties of composites	188:275	Influences of solution preparation method and CN content on the properties of composites were investigated.
26344283	9	46	theme	angle	995:999	arg1	measurements					1001:1012	contact angle measurements	987:1012	contact angle measurements	987:1012	The thermal stability of CS film showed no significant effect of CN addition, whereas contact angle measurements revealed that CN addition resulted in an increment of hydrophilicity of chitosan films.
26344283	0	47	theme	nanocrystal	62:72	arg1	films					74:78	chitosan/cellulose nanocrystal films	43:78	chitosan/cellulose nanocrystal films	43:78	Effects of processing on the properties of chitosan/cellulose nanocrystal films.
26344283	2	48	theme	CN	234:235	arg1	content					237:243	CN content	234:243	CN content	234:243	Influences of solution preparation method and CN content on the properties of composites were investigated.
26344283	5	49	theme	SEM	524:526	arg1	analysis					528:535	SEM analysis	524:535	SEM analysis	524:535	SEM analysis revealed that microfluidization decreased CN aggregates in matrix.
26344283	8	50	theme	53-58°C	850:856	arg1	range					841:845	a range	839:845	a range of 53-58°C	839:856	Tg values within a range of 53-58°C were obtained in DSC and TMA measurements.
27185126	5	0	theme	adsorption	724:733	arg1	capacities					735:744	larger adsorption capacities	717:744	larger adsorption capacities	717:744	It was revealed that the composites pretreated in the solution with higher pH value exhibited larger adsorption capacities.
27185126	2	1	theme	dye	310:312	arg1	removal					314:320	the thymol violet (TV) dye removal	287:320	the thymol violet (TV) dye removal from wastewater	287:336	CS-CMM and TiO2/CS-CMM were used for the thymol violet (TV) dye removal from wastewater.
27185126	8	2	theme	TiO2/CS-CMM	1019:1029	arg1	84.32					1036:1040	84.32	1036:1040	84.32	1036:1040	The calculated maximum adsorption capacities of CS-CMM and TiO2/CS-CMM were 84.32 and 97.51mgg(-1), respectively.
27185126	8	2	theme	TiO2/CS-CMM	1019:1029	arg1	capacities					994:1003	The calculated maximum adsorption capacities	960:1003	The calculated maximum adsorption capacities of CS-CMM and TiO2/CS-CMM	960:1029	The calculated maximum adsorption capacities of CS-CMM and TiO2/CS-CMM were 84.32 and 97.51mgg(-1), respectively.
27185126	8	3	theme	calculated	964:973	arg1	84.32					1036:1040	84.32	1036:1040	84.32	1036:1040	The calculated maximum adsorption capacities of CS-CMM and TiO2/CS-CMM were 84.32 and 97.51mgg(-1), respectively.
27185126	8	3	theme	calculated	964:973	arg1	capacities					994:1003	The calculated maximum adsorption capacities	960:1003	The calculated maximum adsorption capacities of CS-CMM and TiO2/CS-CMM	960:1029	The calculated maximum adsorption capacities of CS-CMM and TiO2/CS-CMM were 84.32 and 97.51mgg(-1), respectively.
27185126	1	4	theme	adsorbent	150:158	arg1	chitosan					117:124	chitosan	117:124	chitosan (TiO2/CS) nanocomposite adsorbent	117:158	A new kind of titanium oxide dispersed in chitosan (TiO2/CS) nanocomposite adsorbent was prepared and adhered to high surface area substrate, cellulose microfibers mat (CMM).
27185126	1	4	theme	adsorbent	150:158	arg1	TiO2/CS					127:133	TiO2/CS	127:133	TiO2/CS	127:133	A new kind of titanium oxide dispersed in chitosan (TiO2/CS) nanocomposite adsorbent was prepared and adhered to high surface area substrate, cellulose microfibers mat (CMM).
27185126	4	5	theme	adsorption	482:491	arg1	function					562:569	a function	560:569	a function of adsorbent dosage, solution pH, and contact time	560:620	The adsorption properties of both the CS-CMM and TiO2/CS-CMM were investigated as a function of adsorbent dosage, solution pH, and contact time.
27185126	4	5	theme	adsorption	482:491	arg1	properties					493:502	The adsorption properties	478:502	The adsorption properties of both the CS-CMM and TiO2/CS-CMM	478:537	The adsorption properties of both the CS-CMM and TiO2/CS-CMM were investigated as a function of adsorbent dosage, solution pH, and contact time.
27185126	8	6	theme	adsorption	983:992	arg1	84.32					1036:1040	84.32	1036:1040	84.32	1036:1040	The calculated maximum adsorption capacities of CS-CMM and TiO2/CS-CMM were 84.32 and 97.51mgg(-1), respectively.
27185126	8	6	theme	adsorption	983:992	arg1	capacities					994:1003	The calculated maximum adsorption capacities	960:1003	The calculated maximum adsorption capacities of CS-CMM and TiO2/CS-CMM	960:1029	The calculated maximum adsorption capacities of CS-CMM and TiO2/CS-CMM were 84.32 and 97.51mgg(-1), respectively.
27185126	5	7	theme	higher	691:696	arg1	value					701:705	higher pH value	691:705	higher pH value	691:705	It was revealed that the composites pretreated in the solution with higher pH value exhibited larger adsorption capacities.
27185126	9	8	theme	nanocomposite	1101:1113	arg1	CMM					1122:1124	the TiO2/CS nanocomposite coated CMM	1089:1124	the TiO2/CS nanocomposite coated CMM	1089:1124	Compare to CS, the TiO2/CS nanocomposite coated CMM showed higher antibacterial characteristics as tested against Escherichia coli.
27185126	7	9	theme	particle	930:937	arg1	mechanism					949:957	involved particle diffusion mechanism	921:957	involved particle diffusion mechanism	921:957	The adsorption process followed pseudo-second order kinetics and involved particle diffusion mechanism.
27185126	3	10	theme	dispersive	447:456	arg1	spectroscopy					464:475	energy dispersive X-ray spectroscopy	440:475	energy dispersive X-ray spectroscopy	440:475	Characterization of materials was carried out by X-ray diffraction, scanning electron microscope and energy dispersive X-ray spectroscopy.
27185126	5	11	theme	pH	698:699	arg1	value					701:705	higher pH value	691:705	higher pH value	691:705	It was revealed that the composites pretreated in the solution with higher pH value exhibited larger adsorption capacities.
27185126	3	12	theme	X-ray	458:462	arg1	spectroscopy					464:475	energy dispersive X-ray spectroscopy	440:475	energy dispersive X-ray spectroscopy	440:475	Characterization of materials was carried out by X-ray diffraction, scanning electron microscope and energy dispersive X-ray spectroscopy.
27185126	4	13	theme	pH	601:602	arg1	function					562:569	a function	560:569	a function of adsorbent dosage, solution pH, and contact time	560:620	The adsorption properties of both the CS-CMM and TiO2/CS-CMM were investigated as a function of adsorbent dosage, solution pH, and contact time.
27185126	4	13	theme	pH	601:602	arg1	properties					493:502	The adsorption properties	478:502	The adsorption properties of both the CS-CMM and TiO2/CS-CMM	478:537	The adsorption properties of both the CS-CMM and TiO2/CS-CMM were investigated as a function of adsorbent dosage, solution pH, and contact time.
27185126	1	14	theme	new	77:79	arg1	kind					81:84	A new kind	75:84	A new kind of titanium oxide dispersed in chitosan (TiO2/CS) nanocomposite adsorbent	75:158	A new kind of titanium oxide dispersed in chitosan (TiO2/CS) nanocomposite adsorbent was prepared and adhered to high surface area substrate, cellulose microfibers mat (CMM).
27185126	4	15	theme	time	617:620	arg1	function					562:569	a function	560:569	a function of adsorbent dosage, solution pH, and contact time	560:620	The adsorption properties of both the CS-CMM and TiO2/CS-CMM were investigated as a function of adsorbent dosage, solution pH, and contact time.
27185126	4	15	theme	time	617:620	arg1	properties					493:502	The adsorption properties	478:502	The adsorption properties of both the CS-CMM and TiO2/CS-CMM	478:537	The adsorption properties of both the CS-CMM and TiO2/CS-CMM were investigated as a function of adsorbent dosage, solution pH, and contact time.
27185126	7	16	theme	involved	921:928	arg1	mechanism					949:957	involved particle diffusion mechanism	921:957	involved particle diffusion mechanism	921:957	The adsorption process followed pseudo-second order kinetics and involved particle diffusion mechanism.
27185126	4	17	theme	contact	609:615	arg1	time					617:620	contact time	609:620	contact time	609:620	The adsorption properties of both the CS-CMM and TiO2/CS-CMM were investigated as a function of adsorbent dosage, solution pH, and contact time.
27185126	0	18	theme	Dye	0:2	arg1	adsorption					4:13	Dye adsorption	0:13	Dye adsorption	0:13	Dye adsorption and bactericidal properties of TiO2/chitosan coating layer.
27185126	4	19	theme	TiO2/CS-CMM	527:537	arg1	function					562:569	a function	560:569	a function of adsorbent dosage, solution pH, and contact time	560:620	The adsorption properties of both the CS-CMM and TiO2/CS-CMM were investigated as a function of adsorbent dosage, solution pH, and contact time.
27185126	4	19	theme	TiO2/CS-CMM	527:537	arg1	properties					493:502	The adsorption properties	478:502	The adsorption properties of both the CS-CMM and TiO2/CS-CMM	478:537	The adsorption properties of both the CS-CMM and TiO2/CS-CMM were investigated as a function of adsorbent dosage, solution pH, and contact time.
27185126	6	20	theme	Kinetic	747:753	arg1	studies					755:761	Kinetic studies	747:761	Kinetic studies	747:761	Kinetic studies showed that the composites could adsorb TV dye rapidly and reached the equilibrium in 90min.
27185126	3	21	theme	X-ray	388:392	arg1	diffraction					394:404	X-ray diffraction	388:404	X-ray diffraction	388:404	Characterization of materials was carried out by X-ray diffraction, scanning electron microscope and energy dispersive X-ray spectroscopy.
27185126	0	22	theme	bactericidal	19:30	arg1	properties					32:41	bactericidal properties	19:41	bactericidal properties	19:41	Dye adsorption and bactericidal properties of TiO2/chitosan coating layer.
27185126	7	23	theme	order	902:906	arg1	kinetics					908:915	pseudo-second order kinetics	888:915	pseudo-second order kinetics	888:915	The adsorption process followed pseudo-second order kinetics and involved particle diffusion mechanism.
27185126	7	24	theme	diffusion	939:947	arg1	mechanism					949:957	involved particle diffusion mechanism	921:957	involved particle diffusion mechanism	921:957	The adsorption process followed pseudo-second order kinetics and involved particle diffusion mechanism.
27185126	1	25	theme	titanium	89:96	arg1	oxide					98:102	titanium oxide	89:102	titanium oxide	89:102	A new kind of titanium oxide dispersed in chitosan (TiO2/CS) nanocomposite adsorbent was prepared and adhered to high surface area substrate, cellulose microfibers mat (CMM).
27185126	1	26	theme	oxide	98:102	arg1	kind					81:84	A new kind	75:84	A new kind of titanium oxide dispersed in chitosan (TiO2/CS) nanocomposite adsorbent	75:158	A new kind of titanium oxide dispersed in chitosan (TiO2/CS) nanocomposite adsorbent was prepared and adhered to high surface area substrate, cellulose microfibers mat (CMM).
27185126	1	27	theme	high	188:191	arg1	area					201:204	high surface area	188:204	high surface area substrate	188:214	A new kind of titanium oxide dispersed in chitosan (TiO2/CS) nanocomposite adsorbent was prepared and adhered to high surface area substrate, cellulose microfibers mat (CMM).
27185126	3	28	theme	scanning	407:414	arg1	microscope					425:434	scanning electron microscope	407:434	scanning electron microscope	407:434	Characterization of materials was carried out by X-ray diffraction, scanning electron microscope and energy dispersive X-ray spectroscopy.
27185126	1	29	theme	surface	193:199	arg1	area					201:204	high surface area	188:204	high surface area substrate	188:214	A new kind of titanium oxide dispersed in chitosan (TiO2/CS) nanocomposite adsorbent was prepared and adhered to high surface area substrate, cellulose microfibers mat (CMM).
27185126	8	30	theme	maximum	975:981	arg1	84.32					1036:1040	84.32	1036:1040	84.32	1036:1040	The calculated maximum adsorption capacities of CS-CMM and TiO2/CS-CMM were 84.32 and 97.51mgg(-1), respectively.
27185126	8	30	theme	maximum	975:981	arg1	capacities					994:1003	The calculated maximum adsorption capacities	960:1003	The calculated maximum adsorption capacities of CS-CMM and TiO2/CS-CMM	960:1029	The calculated maximum adsorption capacities of CS-CMM and TiO2/CS-CMM were 84.32 and 97.51mgg(-1), respectively.
27185126	4	31	theme	solution	592:599	arg1	pH					601:602	solution pH	592:602	solution pH	592:602	The adsorption properties of both the CS-CMM and TiO2/CS-CMM were investigated as a function of adsorbent dosage, solution pH, and contact time.
27185126	9	32	theme	TiO2/CS	1093:1099	arg1	CMM					1122:1124	the TiO2/CS nanocomposite coated CMM	1089:1124	the TiO2/CS nanocomposite coated CMM	1089:1124	Compare to CS, the TiO2/CS nanocomposite coated CMM showed higher antibacterial characteristics as tested against Escherichia coli.
27185126	3	33	theme	electron	416:423	arg1	microscope					425:434	scanning electron microscope	407:434	scanning electron microscope	407:434	Characterization of materials was carried out by X-ray diffraction, scanning electron microscope and energy dispersive X-ray spectroscopy.
27185126	3	34	theme	materials	359:367	arg1	Characterization					339:354	Characterization	339:354	Characterization of materials	339:367	Characterization of materials was carried out by X-ray diffraction, scanning electron microscope and energy dispersive X-ray spectroscopy.
27185126	2	35	from	wastewater	327:336	arg1	removal					314:320	the thymol violet (TV) dye removal	287:320	the thymol violet (TV) dye removal from wastewater	287:336	CS-CMM and TiO2/CS-CMM were used for the thymol violet (TV) dye removal from wastewater.
27185126	2	36	theme	TV	306:307	arg1	dye					310:312	thymol violet (TV) dye	291:312	the thymol violet (TV) dye removal from wastewater	287:336	CS-CMM and TiO2/CS-CMM were used for the thymol violet (TV) dye removal from wastewater.
27185126	0	37	theme	TiO2/chitosan	46:58	arg1	adsorption					4:13	Dye adsorption	0:13	Dye adsorption	0:13	Dye adsorption and bactericidal properties of TiO2/chitosan coating layer.
27185126	0	37	theme	TiO2/chitosan	46:58	arg1	properties					32:41	bactericidal properties	19:41	bactericidal properties	19:41	Dye adsorption and bactericidal properties of TiO2/chitosan coating layer.
27185126	4	38	theme	CS-CMM	516:521	arg1	function					562:569	a function	560:569	a function of adsorbent dosage, solution pH, and contact time	560:620	The adsorption properties of both the CS-CMM and TiO2/CS-CMM were investigated as a function of adsorbent dosage, solution pH, and contact time.
27185126	4	38	theme	CS-CMM	516:521	arg1	properties					493:502	The adsorption properties	478:502	The adsorption properties of both the CS-CMM and TiO2/CS-CMM	478:537	The adsorption properties of both the CS-CMM and TiO2/CS-CMM were investigated as a function of adsorbent dosage, solution pH, and contact time.
27185126	9	39	theme	coated	1115:1120	arg1	CMM					1122:1124	the TiO2/CS nanocomposite coated CMM	1089:1124	the TiO2/CS nanocomposite coated CMM	1089:1124	Compare to CS, the TiO2/CS nanocomposite coated CMM showed higher antibacterial characteristics as tested against Escherichia coli.
27185126	8	40	theme	CS-CMM	1008:1013	arg1	84.32					1036:1040	84.32	1036:1040	84.32	1036:1040	The calculated maximum adsorption capacities of CS-CMM and TiO2/CS-CMM were 84.32 and 97.51mgg(-1), respectively.
27185126	8	40	theme	CS-CMM	1008:1013	arg1	capacities					994:1003	The calculated maximum adsorption capacities	960:1003	The calculated maximum adsorption capacities of CS-CMM and TiO2/CS-CMM	960:1029	The calculated maximum adsorption capacities of CS-CMM and TiO2/CS-CMM were 84.32 and 97.51mgg(-1), respectively.
27185126	2	41	theme	violet	298:303	arg1	dye					310:312	thymol violet (TV) dye	291:312	the thymol violet (TV) dye removal from wastewater	287:336	CS-CMM and TiO2/CS-CMM were used for the thymol violet (TV) dye removal from wastewater.
27185126	2	42	used	used	278:281	arg2	TiO2/CS-CMM					261:271	TiO2/CS-CMM	261:271	TiO2/CS-CMM	261:271	CS-CMM and TiO2/CS-CMM were used for the thymol violet (TV) dye removal from wastewater.
27185126	2	42	used	used	278:281	arg2	CS-CMM					250:255	CS-CMM	250:255	CS-CMM	250:255	CS-CMM and TiO2/CS-CMM were used for the thymol violet (TV) dye removal from wastewater.
27185126	4	43	theme	dosage	584:589	arg1	function					562:569	a function	560:569	a function of adsorbent dosage, solution pH, and contact time	560:620	The adsorption properties of both the CS-CMM and TiO2/CS-CMM were investigated as a function of adsorbent dosage, solution pH, and contact time.
27185126	4	43	theme	dosage	584:589	arg1	properties					493:502	The adsorption properties	478:502	The adsorption properties of both the CS-CMM and TiO2/CS-CMM	478:537	The adsorption properties of both the CS-CMM and TiO2/CS-CMM were investigated as a function of adsorbent dosage, solution pH, and contact time.
27185126	6	44	theme	TV	803:804	arg1	dye					806:808	TV dye	803:808	TV dye	803:808	Kinetic studies showed that the composites could adsorb TV dye rapidly and reached the equilibrium in 90min.
27185126	9	45	theme	antibacterial	1140:1152	arg1	characteristics					1154:1168	higher antibacterial characteristics	1133:1168	higher antibacterial characteristics	1133:1168	Compare to CS, the TiO2/CS nanocomposite coated CMM showed higher antibacterial characteristics as tested against Escherichia coli.
27185126	2	46	theme	thymol	291:296	arg1	dye					310:312	thymol violet (TV) dye	291:312	the thymol violet (TV) dye removal from wastewater	287:336	CS-CMM and TiO2/CS-CMM were used for the thymol violet (TV) dye removal from wastewater.
27185126	7	47	theme	adsorption	860:869	arg1	process					871:877	The adsorption process	856:877	The adsorption process	856:877	The adsorption process followed pseudo-second order kinetics and involved particle diffusion mechanism.
27185126	3	48	theme	energy	440:445	arg1	spectroscopy					464:475	energy dispersive X-ray spectroscopy	440:475	energy dispersive X-ray spectroscopy	440:475	Characterization of materials was carried out by X-ray diffraction, scanning electron microscope and energy dispersive X-ray spectroscopy.
27185126	1	49	theme	area	201:204	arg1	substrate					206:214	high surface area substrate	188:214	high surface area substrate	188:214	A new kind of titanium oxide dispersed in chitosan (TiO2/CS) nanocomposite adsorbent was prepared and adhered to high surface area substrate, cellulose microfibers mat (CMM).
27185126	1	49	theme	area	201:204	arg1	mat					239:241	cellulose microfibers mat	217:241	cellulose microfibers mat (CMM)	217:247	A new kind of titanium oxide dispersed in chitosan (TiO2/CS) nanocomposite adsorbent was prepared and adhered to high surface area substrate, cellulose microfibers mat (CMM).
27185126	9	50	theme	higher	1133:1138	arg1	characteristics					1154:1168	higher antibacterial characteristics	1133:1168	higher antibacterial characteristics	1133:1168	Compare to CS, the TiO2/CS nanocomposite coated CMM showed higher antibacterial characteristics as tested against Escherichia coli.
27185126	7	51	theme	pseudo-second	888:900	arg1	kinetics					908:915	pseudo-second order kinetics	888:915	pseudo-second order kinetics	888:915	The adsorption process followed pseudo-second order kinetics and involved particle diffusion mechanism.
27185126	1	52	theme	cellulose	217:225	arg1	substrate					206:214	high surface area substrate	188:214	high surface area substrate	188:214	A new kind of titanium oxide dispersed in chitosan (TiO2/CS) nanocomposite adsorbent was prepared and adhered to high surface area substrate, cellulose microfibers mat (CMM).
27185126	1	52	theme	cellulose	217:225	arg1	CMM					244:246	CMM	244:246	CMM	244:246	A new kind of titanium oxide dispersed in chitosan (TiO2/CS) nanocomposite adsorbent was prepared and adhered to high surface area substrate, cellulose microfibers mat (CMM).
27185126	1	52	theme	cellulose	217:225	arg1	mat					239:241	cellulose microfibers mat	217:241	cellulose microfibers mat (CMM)	217:247	A new kind of titanium oxide dispersed in chitosan (TiO2/CS) nanocomposite adsorbent was prepared and adhered to high surface area substrate, cellulose microfibers mat (CMM).
27185126	5	53	theme	larger	717:722	arg1	capacities					735:744	larger adsorption capacities	717:744	larger adsorption capacities	717:744	It was revealed that the composites pretreated in the solution with higher pH value exhibited larger adsorption capacities.
27185126	4	54	dep	CS-CMM	516:521	arg1	the					512:514	the	512:514	the	512:514	The adsorption properties of both the CS-CMM and TiO2/CS-CMM were investigated as a function of adsorbent dosage, solution pH, and contact time.
27185126	4	55	theme	adsorbent	574:582	arg1	dosage					584:589	adsorbent dosage	574:589	adsorbent dosage	574:589	The adsorption properties of both the CS-CMM and TiO2/CS-CMM were investigated as a function of adsorbent dosage, solution pH, and contact time.
27185126	1	56	theme	microfibers	227:237	arg1	substrate					206:214	high surface area substrate	188:214	high surface area substrate	188:214	A new kind of titanium oxide dispersed in chitosan (TiO2/CS) nanocomposite adsorbent was prepared and adhered to high surface area substrate, cellulose microfibers mat (CMM).
27185126	1	56	theme	microfibers	227:237	arg1	CMM					244:246	CMM	244:246	CMM	244:246	A new kind of titanium oxide dispersed in chitosan (TiO2/CS) nanocomposite adsorbent was prepared and adhered to high surface area substrate, cellulose microfibers mat (CMM).
27185126	1	56	theme	microfibers	227:237	arg1	mat					239:241	cellulose microfibers mat	217:241	cellulose microfibers mat (CMM)	217:247	A new kind of titanium oxide dispersed in chitosan (TiO2/CS) nanocomposite adsorbent was prepared and adhered to high surface area substrate, cellulose microfibers mat (CMM).
24299835	4	0	theme	membranes	751:759	arg1	hydrophilicity					725:738	hydrophilicity	725:738	hydrophilicity	725:738	The mechanical strength and hydrophilicity of the PEC membranes manufactured from the PEC solutions, especially for a weight ratio of 84/8/8 (chitosan/pectin/gum Arabic), were enhanced compared to pure chitosan membranes.
24299835	4	0	theme	membranes	751:759	arg1	strength					712:719	mechanical strength	701:719	mechanical strength	701:719	The mechanical strength and hydrophilicity of the PEC membranes manufactured from the PEC solutions, especially for a weight ratio of 84/8/8 (chitosan/pectin/gum Arabic), were enhanced compared to pure chitosan membranes.
24299835	1	1	with	solutions	190:198	arg1	compositions					227:238	various compositions	219:238	various compositions	219:238	Novel chitosan/pectin/gum Arabic polyelectrolyte complex (PEC) solutions and membranes with various compositions were prepared for biomedical applications.
24299835	3	2	theme	resultant	534:542	arg1	solutions					548:556	the resultant PEC solutions	530:556	the resultant PEC solutions	530:556	The addition of gum Arabic to the chitosan and pectin significantly decreased the viscosities of the resultant PEC solutions due to the formation of globe-like microstructures that was accompanied by network-like microstructures and other molecular entanglements.
24299835	5	3	theme	drug	1033:1036	arg1	release					1020:1026	steady and fairly complete release	993:1026	steady and fairly complete release of a drug (insulin) for 6h	993:1053	Moreover, the use of the 84/8/8 PEC membranes as a drug carrier exhibited steady and fairly complete release of a drug (insulin) for 6h.
24299835	3	4	theme	PEC	544:546	arg1	solutions					548:556	the resultant PEC solutions	530:556	the resultant PEC solutions	530:556	The addition of gum Arabic to the chitosan and pectin significantly decreased the viscosities of the resultant PEC solutions due to the formation of globe-like microstructures that was accompanied by network-like microstructures and other molecular entanglements.
24299835	6	5	theme	PEC	1121:1123	arg1	membranes					1125:1133	the chitosan/pectin/gum Arabic PEC membranes	1090:1133	the chitosan/pectin/gum Arabic PEC membranes	1090:1133	Based on these promising results, the chitosan/pectin/gum Arabic PEC membranes have great potential in controlled drug release applications.
24299835	4	6	theme	chitosan/pectin/gum	839:857	arg1	84/8/8					831:836	84/8/8	831:836	84/8/8 (chitosan/pectin/gum Arabic)	831:865	The mechanical strength and hydrophilicity of the PEC membranes manufactured from the PEC solutions, especially for a weight ratio of 84/8/8 (chitosan/pectin/gum Arabic), were enhanced compared to pure chitosan membranes.
24299835	4	6	theme	chitosan/pectin/gum	839:857	arg1	Arabic					859:864	chitosan/pectin/gum Arabic	839:864	chitosan/pectin/gum Arabic	839:864	The mechanical strength and hydrophilicity of the PEC membranes manufactured from the PEC solutions, especially for a weight ratio of 84/8/8 (chitosan/pectin/gum Arabic), were enhanced compared to pure chitosan membranes.
24299835	3	7	theme	microstructures	593:607	arg1	formation					569:577	the formation	565:577	the formation of globe-like microstructures that was accompanied by network-like microstructures and other molecular entanglements	565:694	The addition of gum Arabic to the chitosan and pectin significantly decreased the viscosities of the resultant PEC solutions due to the formation of globe-like microstructures that was accompanied by network-like microstructures and other molecular entanglements.
24299835	5	8	theme	complete	1011:1018	arg1	release					1020:1026	steady and fairly complete release	993:1026	steady and fairly complete release of a drug (insulin) for 6h	993:1053	Moreover, the use of the 84/8/8 PEC membranes as a drug carrier exhibited steady and fairly complete release of a drug (insulin) for 6h.
24299835	5	9	theme	84/8/8	944:949	arg1	membranes					955:963	the 84/8/8 PEC membranes	940:963	the 84/8/8 PEC membranes	940:963	Moreover, the use of the 84/8/8 PEC membranes as a drug carrier exhibited steady and fairly complete release of a drug (insulin) for 6h.
24299835	6	10	theme	great	1140:1144	arg1	potential					1146:1154	great potential	1140:1154	great potential	1140:1154	Based on these promising results, the chitosan/pectin/gum Arabic PEC membranes have great potential in controlled drug release applications.
24299835	4	11	theme	PEC	783:785	arg1	solutions					787:795	the PEC solutions	779:795	the PEC solutions	779:795	The mechanical strength and hydrophilicity of the PEC membranes manufactured from the PEC solutions, especially for a weight ratio of 84/8/8 (chitosan/pectin/gum Arabic), were enhanced compared to pure chitosan membranes.
24299835	4	12	theme	chitosan	899:906	arg1	membranes					908:916	pure chitosan membranes	894:916	pure chitosan membranes	894:916	The mechanical strength and hydrophilicity of the PEC membranes manufactured from the PEC solutions, especially for a weight ratio of 84/8/8 (chitosan/pectin/gum Arabic), were enhanced compared to pure chitosan membranes.
24299835	3	13	theme	Arabic	453:458	arg1	addition					437:444	The addition	433:444	The addition of gum Arabic to the chitosan and pectin	433:485	The addition of gum Arabic to the chitosan and pectin significantly decreased the viscosities of the resultant PEC solutions due to the formation of globe-like microstructures that was accompanied by network-like microstructures and other molecular entanglements.
24299835	6	14	theme	chitosan/pectin/gum	1094:1112	arg1	membranes					1125:1133	the chitosan/pectin/gum Arabic PEC membranes	1090:1133	the chitosan/pectin/gum Arabic PEC membranes	1090:1133	Based on these promising results, the chitosan/pectin/gum Arabic PEC membranes have great potential in controlled drug release applications.
24299835	5	15	theme	PEC	951:953	arg1	membranes					955:963	the 84/8/8 PEC membranes	940:963	the 84/8/8 PEC membranes	940:963	Moreover, the use of the 84/8/8 PEC membranes as a drug carrier exhibited steady and fairly complete release of a drug (insulin) for 6h.
24299835	4	16	theme	pure	894:897	arg1	membranes					908:916	pure chitosan membranes	894:916	pure chitosan membranes	894:916	The mechanical strength and hydrophilicity of the PEC membranes manufactured from the PEC solutions, especially for a weight ratio of 84/8/8 (chitosan/pectin/gum Arabic), were enhanced compared to pure chitosan membranes.
24299835	4	17	theme	84/8/8	831:836	arg1	ratio					822:826	a weight ratio	813:826	a weight ratio of 84/8/8 (chitosan/pectin/gum Arabic)	813:865	The mechanical strength and hydrophilicity of the PEC membranes manufactured from the PEC solutions, especially for a weight ratio of 84/8/8 (chitosan/pectin/gum Arabic), were enhanced compared to pure chitosan membranes.
24299835	6	18	theme	Arabic	1114:1119	arg1	membranes					1125:1133	the chitosan/pectin/gum Arabic PEC membranes	1090:1133	the chitosan/pectin/gum Arabic PEC membranes	1090:1133	Based on these promising results, the chitosan/pectin/gum Arabic PEC membranes have great potential in controlled drug release applications.
24299835	0	19	theme	Arabic	20:25	arg1	complex					43:49	Chitosan/pectin/gum Arabic polyelectrolyte complex	0:49	Chitosan/pectin/gum Arabic polyelectrolyte complex: process-dependent appearance, microstructure analysis and its application.	0:125	Chitosan/pectin/gum Arabic polyelectrolyte complex: process-dependent appearance, microstructure analysis and its application.
24299835	4	20	theme	weight	815:820	arg1	ratio					822:826	a weight ratio	813:826	a weight ratio of 84/8/8 (chitosan/pectin/gum Arabic)	813:865	The mechanical strength and hydrophilicity of the PEC membranes manufactured from the PEC solutions, especially for a weight ratio of 84/8/8 (chitosan/pectin/gum Arabic), were enhanced compared to pure chitosan membranes.
24299835	1	21	theme	various	219:225	arg1	compositions					227:238	various compositions	219:238	various compositions	219:238	Novel chitosan/pectin/gum Arabic polyelectrolyte complex (PEC) solutions and membranes with various compositions were prepared for biomedical applications.
24299835	0	22	theme	Chitosan/pectin/gum	0:18	arg1	complex					43:49	Chitosan/pectin/gum Arabic polyelectrolyte complex	0:49	Chitosan/pectin/gum Arabic polyelectrolyte complex: process-dependent appearance, microstructure analysis and its application.	0:125	Chitosan/pectin/gum Arabic polyelectrolyte complex: process-dependent appearance, microstructure analysis and its application.
24299835	6	23	contain	have	1135:1138	arg1	membranes					1125:1133	the chitosan/pectin/gum Arabic PEC membranes	1090:1133	the chitosan/pectin/gum Arabic PEC membranes	1090:1133	Based on these promising results, the chitosan/pectin/gum Arabic PEC membranes have great potential in controlled drug release applications.
24299835	6	23	contain	have	1135:1138	arg2	potential					1146:1154	great potential	1140:1154	great potential	1140:1154	Based on these promising results, the chitosan/pectin/gum Arabic PEC membranes have great potential in controlled drug release applications.
24299835	3	24	theme	molecular	672:680	arg1	entanglements					682:694	other molecular entanglements	666:694	other molecular entanglements	666:694	The addition of gum Arabic to the chitosan and pectin significantly decreased the viscosities of the resultant PEC solutions due to the formation of globe-like microstructures that was accompanied by network-like microstructures and other molecular entanglements.
24299835	1	25	theme	Novel	127:131	arg1	complex					176:182	Novel chitosan/pectin/gum Arabic polyelectrolyte complex	127:182	Novel chitosan/pectin/gum Arabic polyelectrolyte complex (PEC) solutions	127:198	Novel chitosan/pectin/gum Arabic polyelectrolyte complex (PEC) solutions and membranes with various compositions were prepared for biomedical applications.
24299835	1	25	theme	Novel	127:131	arg1	PEC					185:187	PEC	185:187	PEC	185:187	Novel chitosan/pectin/gum Arabic polyelectrolyte complex (PEC) solutions and membranes with various compositions were prepared for biomedical applications.
24299835	4	26	dep	strength	712:719	arg1	The					697:699	The	697:699	The	697:699	The mechanical strength and hydrophilicity of the PEC membranes manufactured from the PEC solutions, especially for a weight ratio of 84/8/8 (chitosan/pectin/gum Arabic), were enhanced compared to pure chitosan membranes.
24299835	6	27	theme	promising	1071:1079	arg1	results					1081:1087	these promising results	1065:1087	these promising results	1065:1087	Based on these promising results, the chitosan/pectin/gum Arabic PEC membranes have great potential in controlled drug release applications.
24299835	0	28	theme	polyelectrolyte	27:41	arg1	complex					43:49	Chitosan/pectin/gum Arabic polyelectrolyte complex	0:49	Chitosan/pectin/gum Arabic polyelectrolyte complex: process-dependent appearance, microstructure analysis and its application.	0:125	Chitosan/pectin/gum Arabic polyelectrolyte complex: process-dependent appearance, microstructure analysis and its application.
24299835	1	29	theme	chitosan/pectin/gum	133:151	arg1	complex					176:182	Novel chitosan/pectin/gum Arabic polyelectrolyte complex	127:182	Novel chitosan/pectin/gum Arabic polyelectrolyte complex (PEC) solutions	127:198	Novel chitosan/pectin/gum Arabic polyelectrolyte complex (PEC) solutions and membranes with various compositions were prepared for biomedical applications.
24299835	1	29	theme	chitosan/pectin/gum	133:151	arg1	PEC					185:187	PEC	185:187	PEC	185:187	Novel chitosan/pectin/gum Arabic polyelectrolyte complex (PEC) solutions and membranes with various compositions were prepared for biomedical applications.
24299835	3	30	theme	solutions	548:556	arg1	viscosities					515:525	the viscosities	511:525	the viscosities of the resultant PEC solutions	511:556	The addition of gum Arabic to the chitosan and pectin significantly decreased the viscosities of the resultant PEC solutions due to the formation of globe-like microstructures that was accompanied by network-like microstructures and other molecular entanglements.
24299835	0	31	theme	process-dependent	52:68	arg1	appearance					70:79	process-dependent appearance	52:79	process-dependent appearance	52:79	Chitosan/pectin/gum Arabic polyelectrolyte complex: process-dependent appearance, microstructure analysis and its application.
24299835	3	32	theme	network-like	633:644	arg1	microstructures					646:660	network-like microstructures	633:660	network-like microstructures	633:660	The addition of gum Arabic to the chitosan and pectin significantly decreased the viscosities of the resultant PEC solutions due to the formation of globe-like microstructures that was accompanied by network-like microstructures and other molecular entanglements.
24299835	2	33	dep	appearance	287:296	arg1	either					320:325	either	320:325	either	320:325	The appearance of the PEC solutions, either clear or turbid, was process-dependent and depended on how the three components were dissolved and mixed.
24299835	2	33	dep	appearance	287:296	arg1	turbid					336:341	turbid	336:341	turbid	336:341	The appearance of the PEC solutions, either clear or turbid, was process-dependent and depended on how the three components were dissolved and mixed.
24299835	3	34	theme	gum	449:451	arg1	Arabic					453:458	gum Arabic	449:458	gum Arabic	449:458	The addition of gum Arabic to the chitosan and pectin significantly decreased the viscosities of the resultant PEC solutions due to the formation of globe-like microstructures that was accompanied by network-like microstructures and other molecular entanglements.
24299835	1	35	theme	biomedical	258:267	arg1	applications					269:280	biomedical applications	258:280	biomedical applications	258:280	Novel chitosan/pectin/gum Arabic polyelectrolyte complex (PEC) solutions and membranes with various compositions were prepared for biomedical applications.
24299835	1	36	theme	Arabic	153:158	arg1	complex					176:182	Novel chitosan/pectin/gum Arabic polyelectrolyte complex	127:182	Novel chitosan/pectin/gum Arabic polyelectrolyte complex (PEC) solutions	127:198	Novel chitosan/pectin/gum Arabic polyelectrolyte complex (PEC) solutions and membranes with various compositions were prepared for biomedical applications.
24299835	1	36	theme	Arabic	153:158	arg1	PEC					185:187	PEC	185:187	PEC	185:187	Novel chitosan/pectin/gum Arabic polyelectrolyte complex (PEC) solutions and membranes with various compositions were prepared for biomedical applications.
24299835	5	37	theme	steady	993:998	arg1	release					1020:1026	steady and fairly complete release	993:1026	steady and fairly complete release of a drug (insulin) for 6h	993:1053	Moreover, the use of the 84/8/8 PEC membranes as a drug carrier exhibited steady and fairly complete release of a drug (insulin) for 6h.
24299835	6	38	theme	release	1175:1181	arg1	applications					1183:1194	controlled drug release applications	1159:1194	controlled drug release applications	1159:1194	Based on these promising results, the chitosan/pectin/gum Arabic PEC membranes have great potential in controlled drug release applications.
24299835	1	39	theme	polyelectrolyte	160:174	arg1	complex					176:182	Novel chitosan/pectin/gum Arabic polyelectrolyte complex	127:182	Novel chitosan/pectin/gum Arabic polyelectrolyte complex (PEC) solutions	127:198	Novel chitosan/pectin/gum Arabic polyelectrolyte complex (PEC) solutions and membranes with various compositions were prepared for biomedical applications.
24299835	1	39	theme	polyelectrolyte	160:174	arg1	PEC					185:187	PEC	185:187	PEC	185:187	Novel chitosan/pectin/gum Arabic polyelectrolyte complex (PEC) solutions and membranes with various compositions were prepared for biomedical applications.
24299835	1	40	with	membranes	204:212	arg1	compositions					227:238	various compositions	219:238	various compositions	219:238	Novel chitosan/pectin/gum Arabic polyelectrolyte complex (PEC) solutions and membranes with various compositions were prepared for biomedical applications.
24299835	2	41	theme	solutions	309:317	arg1	appearance					287:296	The appearance	283:296	The appearance	283:296	The appearance of the PEC solutions, either clear or turbid, was process-dependent and depended on how the three components were dissolved and mixed.
24299835	2	41	theme	solutions	309:317	arg1	process-dependent					348:364	process-dependent	348:364	process-dependent	348:364	The appearance of the PEC solutions, either clear or turbid, was process-dependent and depended on how the three components were dissolved and mixed.
24299835	0	42	dep	complex	43:49	arg1	appearance					70:79	process-dependent appearance	52:79	process-dependent appearance	52:79	Chitosan/pectin/gum Arabic polyelectrolyte complex: process-dependent appearance, microstructure analysis and its application.
24299835	0	42	dep	complex	43:49	arg1	analysis					97:104	microstructure analysis	82:104	microstructure analysis	82:104	Chitosan/pectin/gum Arabic polyelectrolyte complex: process-dependent appearance, microstructure analysis and its application.
24299835	0	42	dep	complex	43:49	arg1	application					114:124	its application	110:124	its application	110:124	Chitosan/pectin/gum Arabic polyelectrolyte complex: process-dependent appearance, microstructure analysis and its application.
24299835	1	43	theme	complex	176:182	arg1	solutions					190:198	Novel chitosan/pectin/gum Arabic polyelectrolyte complex (PEC) solutions	127:198	Novel chitosan/pectin/gum Arabic polyelectrolyte complex (PEC) solutions	127:198	Novel chitosan/pectin/gum Arabic polyelectrolyte complex (PEC) solutions and membranes with various compositions were prepared for biomedical applications.
24299835	3	44	theme	globe-like	582:591	arg1	microstructures					593:607	globe-like microstructures	582:607	globe-like microstructures that was accompanied by network-like microstructures and other molecular entanglements	582:694	The addition of gum Arabic to the chitosan and pectin significantly decreased the viscosities of the resultant PEC solutions due to the formation of globe-like microstructures that was accompanied by network-like microstructures and other molecular entanglements.
24299835	5	45	theme	membranes	955:963	arg1	use					933:935	the use	929:935	the use of the 84/8/8 PEC membranes as a drug carrier	929:981	Moreover, the use of the 84/8/8 PEC membranes as a drug carrier exhibited steady and fairly complete release of a drug (insulin) for 6h.
24299835	2	46	theme	PEC	305:307	arg1	solutions					309:317	the PEC solutions	301:317	the PEC solutions	301:317	The appearance of the PEC solutions, either clear or turbid, was process-dependent and depended on how the three components were dissolved and mixed.
24299835	5	47	theme	drug	970:973	arg1	carrier					975:981	a drug carrier	968:981	a drug carrier	968:981	Moreover, the use of the 84/8/8 PEC membranes as a drug carrier exhibited steady and fairly complete release of a drug (insulin) for 6h.
24299835	6	48	theme	controlled	1159:1168	arg1	applications					1183:1194	controlled drug release applications	1159:1194	controlled drug release applications	1159:1194	Based on these promising results, the chitosan/pectin/gum Arabic PEC membranes have great potential in controlled drug release applications.
24299835	4	49	theme	mechanical	701:710	arg1	strength					712:719	mechanical strength	701:719	mechanical strength	701:719	The mechanical strength and hydrophilicity of the PEC membranes manufactured from the PEC solutions, especially for a weight ratio of 84/8/8 (chitosan/pectin/gum Arabic), were enhanced compared to pure chitosan membranes.
24299835	2	50	dep	either	320:325	arg1	clear					327:331	clear	327:331	clear	327:331	The appearance of the PEC solutions, either clear or turbid, was process-dependent and depended on how the three components were dissolved and mixed.
24299835	0	51	theme	microstructure	82:95	arg1	analysis					97:104	microstructure analysis	82:104	microstructure analysis	82:104	Chitosan/pectin/gum Arabic polyelectrolyte complex: process-dependent appearance, microstructure analysis and its application.
24299835	6	52	theme	drug	1170:1173	arg1	applications					1183:1194	controlled drug release applications	1159:1194	controlled drug release applications	1159:1194	Based on these promising results, the chitosan/pectin/gum Arabic PEC membranes have great potential in controlled drug release applications.
24299835	3	53	theme	other	666:670	arg1	entanglements					682:694	other molecular entanglements	666:694	other molecular entanglements	666:694	The addition of gum Arabic to the chitosan and pectin significantly decreased the viscosities of the resultant PEC solutions due to the formation of globe-like microstructures that was accompanied by network-like microstructures and other molecular entanglements.
24299835	4	54	theme	PEC	747:749	arg1	membranes					751:759	the PEC membranes	743:759	the PEC membranes manufactured from the PEC solutions	743:795	The mechanical strength and hydrophilicity of the PEC membranes manufactured from the PEC solutions, especially for a weight ratio of 84/8/8 (chitosan/pectin/gum Arabic), were enhanced compared to pure chitosan membranes.
28267508	4	0	theme	GG-Gly	547:552	arg1	biocompatibility					554:569	the GG-Gly biocompatibility	543:569	the GG-Gly biocompatibility	543:569	HNT incorporation within the hydrogel offers the versatility to improve the GG-Gly biocompatibility with potential incorporation of target biomolecules.
28267508	8	1	theme	%	1047:1047	arg1	HNT					1049:1051	25% HNT	1045:1051	25% HNT	1045:1051	Finally, in vitro study with human fibroblasts on GG-Gly hydrogels loaded with 25% HNT offered the higher metabolic activities and cell survival up to 7days of incubation.
28267508	5	2	with	hydrogels	638:646	arg1	ratios					675:680	different composition ratios	653:680	different composition ratios	653:680	In this work, hydrogels with different composition ratios are physically crosslinked for tuning physico-mechanical properties.
28267508	0	3	theme	engineering	78:88	arg1	applications					90:101	soft tissue engineering applications	66:101	soft tissue engineering applications	66:101	Insight into halloysite nanotubes-loaded gellan gum hydrogels for soft tissue engineering applications.
28267508	8	4	theme	25	1045:1046	arg1	%					1047:1047	%	1047:1047	%	1047:1047	Finally, in vitro study with human fibroblasts on GG-Gly hydrogels loaded with 25% HNT offered the higher metabolic activities and cell survival up to 7days of incubation.
28267508	8	5	theme	human	995:999	arg1	fibroblasts					1001:1011	human fibroblasts	995:1011	human fibroblasts	995:1011	Finally, in vitro study with human fibroblasts on GG-Gly hydrogels loaded with 25% HNT offered the higher metabolic activities and cell survival up to 7days of incubation.
28267508	5	6	theme	different	653:661	arg1	ratios					675:680	different composition ratios	653:680	different composition ratios	653:680	In this work, hydrogels with different composition ratios are physically crosslinked for tuning physico-mechanical properties.
28267508	7	7	theme	compressive	908:918	arg1	modulus					928:934	a compressive Young's modulus	906:934	a compressive Young's modulus ranging between 20 and 75kPa	906:963	HNT addition leads to a water uptake decrease of 30-35% and tuneable mechanical properties with a compressive Young's modulus ranging between 20 and 75kPa.
28267508	8	8	theme	in	975:976	arg1	study					984:988	in vitro study	975:988	in vitro study with human fibroblasts on GG-Gly hydrogels loaded with 25% HNT	975:1051	Finally, in vitro study with human fibroblasts on GG-Gly hydrogels loaded with 25% HNT offered the higher metabolic activities and cell survival up to 7days of incubation.
28267508	5	9	theme	composition	663:673	arg1	ratios					675:680	different composition ratios	653:680	different composition ratios	653:680	In this work, hydrogels with different composition ratios are physically crosslinked for tuning physico-mechanical properties.
28267508	8	10	dep	activities	1082:1091	arg1	the					1061:1063	the	1061:1063	the	1061:1063	Finally, in vitro study with human fibroblasts on GG-Gly hydrogels loaded with 25% HNT offered the higher metabolic activities and cell survival up to 7days of incubation.
28267508	4	11	theme	potential	576:584	arg1	incorporation					586:598	potential incorporation	576:598	potential incorporation of target biomolecules	576:621	HNT incorporation within the hydrogel offers the versatility to improve the GG-Gly biocompatibility with potential incorporation of target biomolecules.
28267508	8	12	theme	higher	1065:1070	arg1	activities					1082:1091	higher metabolic activities	1065:1091	higher metabolic activities	1065:1091	Finally, in vitro study with human fibroblasts on GG-Gly hydrogels loaded with 25% HNT offered the higher metabolic activities and cell survival up to 7days of incubation.
28267508	2	13	theme	biomimetic	360:369	arg1	features					371:378	its biomimetic features	356:378	its biomimetic features	356:378	The FDA-approved GG polysaccharide has been recently exploited as biomaterial because its biomimetic features.
28267508	8	14	with	study	984:988	arg1	fibroblasts					1001:1011	human fibroblasts	995:1011	human fibroblasts	995:1011	Finally, in vitro study with human fibroblasts on GG-Gly hydrogels loaded with 25% HNT offered the higher metabolic activities and cell survival up to 7days of incubation.
28267508	2	15	theme	FDA-approved	274:285	arg1	polysaccharide					290:303	The FDA-approved GG polysaccharide	270:303	The FDA-approved GG polysaccharide	270:303	The FDA-approved GG polysaccharide has been recently exploited as biomaterial because its biomimetic features.
28267508	2	15	theme	FDA-approved	274:285	arg1	biomaterial					336:346	biomaterial	336:346	biomaterial	336:346	The FDA-approved GG polysaccharide has been recently exploited as biomaterial because its biomimetic features.
28267508	7	16	theme	tuneable	870:877	arg1	properties					890:899	tuneable mechanical properties	870:899	tuneable mechanical properties with a compressive Young's modulus ranging between 20 and 75kPa	870:963	HNT addition leads to a water uptake decrease of 30-35% and tuneable mechanical properties with a compressive Young's modulus ranging between 20 and 75kPa.
28267508	7	17	theme	water	834:838	arg1	decrease					847:854	a water uptake decrease	832:854	a water uptake decrease of 30-35%	832:864	HNT addition leads to a water uptake decrease of 30-35% and tuneable mechanical properties with a compressive Young's modulus ranging between 20 and 75kPa.
28267508	8	18	theme	GG-Gly	1016:1021	arg1	hydrogels					1023:1031	GG-Gly hydrogels	1016:1031	GG-Gly hydrogels loaded with 25% HNT	1016:1051	Finally, in vitro study with human fibroblasts on GG-Gly hydrogels loaded with 25% HNT offered the higher metabolic activities and cell survival up to 7days of incubation.
28267508	7	19	theme	mechanical	879:888	arg1	properties					890:899	tuneable mechanical properties	870:899	tuneable mechanical properties with a compressive Young's modulus ranging between 20 and 75kPa	870:963	HNT addition leads to a water uptake decrease of 30-35% and tuneable mechanical properties with a compressive Young's modulus ranging between 20 and 75kPa.
28267508	7	20	theme	uptake	840:845	arg1	decrease					847:854	a water uptake decrease	832:854	a water uptake decrease of 30-35%	832:864	HNT addition leads to a water uptake decrease of 30-35% and tuneable mechanical properties with a compressive Young's modulus ranging between 20 and 75kPa.
28267508	8	21	theme	metabolic	1072:1080	arg1	activities					1082:1091	higher metabolic activities	1065:1091	higher metabolic activities	1065:1091	Finally, in vitro study with human fibroblasts on GG-Gly hydrogels loaded with 25% HNT offered the higher metabolic activities and cell survival up to 7days of incubation.
28267508	7	22	theme	%	864:864	arg1	properties					890:899	tuneable mechanical properties	870:899	tuneable mechanical properties with a compressive Young's modulus ranging between 20 and 75kPa	870:963	HNT addition leads to a water uptake decrease of 30-35% and tuneable mechanical properties with a compressive Young's modulus ranging between 20 and 75kPa.
28267508	7	22	theme	%	864:864	arg1	decrease					847:854	a water uptake decrease	832:854	a water uptake decrease of 30-35%	832:864	HNT addition leads to a water uptake decrease of 30-35% and tuneable mechanical properties with a compressive Young's modulus ranging between 20 and 75kPa.
28267508	4	23	theme	HNT	471:473	arg1	incorporation					475:487	HNT incorporation	471:487	HNT incorporation within the hydrogel	471:507	HNT incorporation within the hydrogel offers the versatility to improve the GG-Gly biocompatibility with potential incorporation of target biomolecules.
28267508	8	24	theme	cell	1097:1100	arg1	survival					1102:1109	cell survival	1097:1109	cell survival	1097:1109	Finally, in vitro study with human fibroblasts on GG-Gly hydrogels loaded with 25% HNT offered the higher metabolic activities and cell survival up to 7days of incubation.
28267508	1	25	theme	tri-component	106:118	arg1	hydrogel					120:127	A tri-component hydrogel	104:127	A tri-component hydrogel	104:127	A tri-component hydrogel, based on gellan gum (GG), glycerol (Gly) and halloysite nanotubes (HNT), is proposed in this work for soft tissue engineering applications.
28267508	3	26	theme	hydrogel	425:432	arg1	viscosity					434:442	hydrogel viscosity	425:442	hydrogel viscosity	425:442	Gly is added as molecular spacer to improve hydrogel viscosity and mechanical properties.
28267508	1	27	theme	soft	232:235	arg1	applications					256:267	soft tissue engineering applications	232:267	soft tissue engineering applications	232:267	A tri-component hydrogel, based on gellan gum (GG), glycerol (Gly) and halloysite nanotubes (HNT), is proposed in this work for soft tissue engineering applications.
28267508	5	28	theme	tuning	713:718	arg1	properties					739:748	tuning physico-mechanical properties	713:748	tuning physico-mechanical properties	713:748	In this work, hydrogels with different composition ratios are physically crosslinked for tuning physico-mechanical properties.
28267508	5	29	attach	crosslinked	697:707	arg3	work					632:635	this work	627:635	this work	627:635	In this work, hydrogels with different composition ratios are physically crosslinked for tuning physico-mechanical properties.
28267508	0	30	theme	gum	48:50	arg1	hydrogels					52:60	gellan gum hydrogels	41:60	gellan gum hydrogels for soft tissue engineering applications	41:101	Insight into halloysite nanotubes-loaded gellan gum hydrogels for soft tissue engineering applications.
28267508	1	31	theme	tissue	237:242	arg1	applications					256:267	soft tissue engineering applications	232:267	soft tissue engineering applications	232:267	A tri-component hydrogel, based on gellan gum (GG), glycerol (Gly) and halloysite nanotubes (HNT), is proposed in this work for soft tissue engineering applications.
28267508	5	32	theme	physico-mechanical	720:737	arg1	properties					739:748	tuning physico-mechanical properties	713:748	tuning physico-mechanical properties	713:748	In this work, hydrogels with different composition ratios are physically crosslinked for tuning physico-mechanical properties.
28267508	1	33	theme	halloysite	175:184	arg1	HNT					197:199	HNT	197:199	HNT	197:199	A tri-component hydrogel, based on gellan gum (GG), glycerol (Gly) and halloysite nanotubes (HNT), is proposed in this work for soft tissue engineering applications.
28267508	1	33	theme	halloysite	175:184	arg1	nanotubes					186:194	halloysite nanotubes	175:194	halloysite nanotubes (HNT)	175:200	A tri-component hydrogel, based on gellan gum (GG), glycerol (Gly) and halloysite nanotubes (HNT), is proposed in this work for soft tissue engineering applications.
28267508	0	34	theme	gellan	41:46	arg1	hydrogels					52:60	gellan gum hydrogels	41:60	gellan gum hydrogels for soft tissue engineering applications	41:101	Insight into halloysite nanotubes-loaded gellan gum hydrogels for soft tissue engineering applications.
28267508	1	35	theme	engineering	244:254	arg1	applications					256:267	soft tissue engineering applications	232:267	soft tissue engineering applications	232:267	A tri-component hydrogel, based on gellan gum (GG), glycerol (Gly) and halloysite nanotubes (HNT), is proposed in this work for soft tissue engineering applications.
28267508	8	36	dep	7days	1117:1121	arg1	up					1111:1112	up	1111:1112	up	1111:1112	Finally, in vitro study with human fibroblasts on GG-Gly hydrogels loaded with 25% HNT offered the higher metabolic activities and cell survival up to 7days of incubation.
28267508	3	37	theme	molecular	397:405	arg1	spacer					407:412	molecular spacer	397:412	molecular spacer to improve hydrogel viscosity and mechanical properties	397:468	Gly is added as molecular spacer to improve hydrogel viscosity and mechanical properties.
28267508	3	37	theme	molecular	397:405	arg1	Gly					381:383	Gly	381:383	Gly	381:383	Gly is added as molecular spacer to improve hydrogel viscosity and mechanical properties.
28267508	7	38	theme	HNT	810:812	arg1	addition					814:821	HNT addition	810:821	HNT addition	810:821	HNT addition leads to a water uptake decrease of 30-35% and tuneable mechanical properties with a compressive Young's modulus ranging between 20 and 75kPa.
28267508	8	39	theme	incubation	1126:1135	arg1	7days					1117:1121	7days	1117:1121	7days of incubation	1117:1135	Finally, in vitro study with human fibroblasts on GG-Gly hydrogels loaded with 25% HNT offered the higher metabolic activities and cell survival up to 7days of incubation.
28267508	2	40	theme	GG	287:288	arg1	polysaccharide					290:303	The FDA-approved GG polysaccharide	270:303	The FDA-approved GG polysaccharide	270:303	The FDA-approved GG polysaccharide has been recently exploited as biomaterial because its biomimetic features.
28267508	2	40	theme	GG	287:288	arg1	biomaterial					336:346	biomaterial	336:346	biomaterial	336:346	The FDA-approved GG polysaccharide has been recently exploited as biomaterial because its biomimetic features.
28267508	4	41	theme	target	603:608	arg1	biomolecules					610:621	target biomolecules	603:621	target biomolecules	603:621	HNT incorporation within the hydrogel offers the versatility to improve the GG-Gly biocompatibility with potential incorporation of target biomolecules.
28267508	6	42	theme	physico-chemical	763:778	arg1	characterization					780:795	An accurate physico-chemical characterization	751:795	An accurate physico-chemical characterization	751:795	An accurate physico-chemical characterization is reported.
28267508	1	43	theme	gellan	139:144	arg1	GG					151:152	GG	151:152	GG	151:152	A tri-component hydrogel, based on gellan gum (GG), glycerol (Gly) and halloysite nanotubes (HNT), is proposed in this work for soft tissue engineering applications.
28267508	1	43	theme	gellan	139:144	arg1	gum					146:148	gellan gum	139:148	gellan gum (GG)	139:153	A tri-component hydrogel, based on gellan gum (GG), glycerol (Gly) and halloysite nanotubes (HNT), is proposed in this work for soft tissue engineering applications.
28267508	8	44	dep	in	975:976	arg1	vitro					978:982	vitro	978:982	vitro	978:982	Finally, in vitro study with human fibroblasts on GG-Gly hydrogels loaded with 25% HNT offered the higher metabolic activities and cell survival up to 7days of incubation.
28267508	8	45	from	study	984:988	arg1	hydrogels					1023:1031	GG-Gly hydrogels	1016:1031	GG-Gly hydrogels loaded with 25% HNT	1016:1051	Finally, in vitro study with human fibroblasts on GG-Gly hydrogels loaded with 25% HNT offered the higher metabolic activities and cell survival up to 7days of incubation.
28267508	0	46	theme	tissue	71:76	arg1	applications					90:101	soft tissue engineering applications	66:101	soft tissue engineering applications	66:101	Insight into halloysite nanotubes-loaded gellan gum hydrogels for soft tissue engineering applications.
28267508	6	47	theme	accurate	754:761	arg1	characterization					780:795	An accurate physico-chemical characterization	751:795	An accurate physico-chemical characterization	751:795	An accurate physico-chemical characterization is reported.
28267508	3	48	theme	mechanical	448:457	arg1	properties					459:468	mechanical properties	448:468	mechanical properties	448:468	Gly is added as molecular spacer to improve hydrogel viscosity and mechanical properties.
28267508	0	49	theme	soft	66:69	arg1	applications					90:101	soft tissue engineering applications	66:101	soft tissue engineering applications	66:101	Insight into halloysite nanotubes-loaded gellan gum hydrogels for soft tissue engineering applications.
28267508	7	50	theme	Young	920:924	arg1	modulus					928:934	a compressive Young's modulus	906:934	a compressive Young's modulus ranging between 20 and 75kPa	906:963	HNT addition leads to a water uptake decrease of 30-35% and tuneable mechanical properties with a compressive Young's modulus ranging between 20 and 75kPa.
28267508	4	51	theme	biomolecules	610:621	arg1	incorporation					586:598	potential incorporation	576:598	potential incorporation of target biomolecules	576:621	HNT incorporation within the hydrogel offers the versatility to improve the GG-Gly biocompatibility with potential incorporation of target biomolecules.
27801570	0	0	theme	Chemoattractant	100:114	arg1	Protein-1					116:124	Monocyte Chemoattractant Protein-1	91:124	Monocyte Chemoattractant Protein-1	91:124	Enrichment of Two Isomeric Heparin Oligosaccharides Exhibiting Different Affinities toward Monocyte Chemoattractant Protein-1.
27801570	8	1	theme	heparan	1556:1562	arg1	sulfate					1564:1570	heparan sulfate	1556:1570	heparan sulfate protein binding	1556:1586	These data indicate sequence specificity for high affinity binding, supporting the view that sulfate position, and not simply the number of sulfates, is important for heparan sulfate protein binding.
27801570	5	2	dep	70	912:913	arg1	to					909:910	to	909:910	to	909:910	A significant shift in arrival time distribution of close to 70 Å2 was observed, indicating a more compact protein:hexasaccharide conformation.
27801570	2	3	theme	Monocyte	428:435	arg1	MCP-1					464:468	MCP-1	464:468	MCP-1	464:468	Here we demonstrate chromatographic isolation and purification of two heparin hexasaccharide isomers that interact with the oligomeric chemokine Monocyte Chemoattractant Protein-1 (MCP-1)/CCL2 with different binding affinities.
27801570	2	3	theme	Monocyte	428:435	arg1	Protein-1					453:461	oligomeric chemokine Monocyte Chemoattractant Protein-1	407:461	the oligomeric chemokine Monocyte Chemoattractant Protein-1 (MCP-1)/CCL2 with different binding affinities	403:508	Here we demonstrate chromatographic isolation and purification of two heparin hexasaccharide isomers that interact with the oligomeric chemokine Monocyte Chemoattractant Protein-1 (MCP-1)/CCL2 with different binding affinities.
27801570	6	4	theme	values	1091:1096	arg1	calculation					1073:1083	calculation	1073:1083	calculation of Kd values	1073:1096	Clear differences in the MS spectra between bound and unbound protein allowed calculation of Kd values from the resulting data.
27801570	0	5	theme	Monocyte	91:98	arg1	Protein-1					116:124	Monocyte Chemoattractant Protein-1	91:124	Monocyte Chemoattractant Protein-1	91:124	Enrichment of Two Isomeric Heparin Oligosaccharides Exhibiting Different Affinities toward Monocyte Chemoattractant Protein-1.
27801570	2	6	with	/CCL2	470:474	arg1	affinities					499:508	different binding affinities	481:508	different binding affinities	481:508	Here we demonstrate chromatographic isolation and purification of two heparin hexasaccharide isomers that interact with the oligomeric chemokine Monocyte Chemoattractant Protein-1 (MCP-1)/CCL2 with different binding affinities.
27801570	2	7	theme	chemokine	418:426	arg1	MCP-1					464:468	MCP-1	464:468	MCP-1	464:468	Here we demonstrate chromatographic isolation and purification of two heparin hexasaccharide isomers that interact with the oligomeric chemokine Monocyte Chemoattractant Protein-1 (MCP-1)/CCL2 with different binding affinities.
27801570	2	7	theme	chemokine	418:426	arg1	Protein-1					453:461	oligomeric chemokine Monocyte Chemoattractant Protein-1	407:461	the oligomeric chemokine Monocyte Chemoattractant Protein-1 (MCP-1)/CCL2 with different binding affinities	403:508	Here we demonstrate chromatographic isolation and purification of two heparin hexasaccharide isomers that interact with the oligomeric chemokine Monocyte Chemoattractant Protein-1 (MCP-1)/CCL2 with different binding affinities.
27801570	8	8	theme	protein	1572:1578	arg1	binding					1580:1586	heparan sulfate protein binding	1556:1586	heparan sulfate protein binding	1556:1586	These data indicate sequence specificity for high affinity binding, supporting the view that sulfate position, and not simply the number of sulfates, is important for heparan sulfate protein binding.
27801570	6	9	theme	Kd	1088:1089	arg1	values					1091:1096	Kd values	1088:1096	Kd values	1088:1096	Clear differences in the MS spectra between bound and unbound protein allowed calculation of Kd values from the resulting data.
27801570	7	10	theme	uronic	1281:1286	arg1	acid					1288:1291	uronic acid	1281:1291	uronic acid	1281:1291	The structural difference between the two hexasaccharides was defined as the differential location of a single sulfate at either C-6 of glucosamine or C-2 of uronic acid in the reducing disaccharide, resulting in a 200-fold difference in binding affinity for MCP-1.
27801570	6	11	theme	Clear	995:999	arg1	differences					1001:1011	Clear differences	995:1011	Clear differences in the MS spectra between bound and unbound protein	995:1063	Clear differences in the MS spectra between bound and unbound protein allowed calculation of Kd values from the resulting data.
27801570	7	12	theme	differential	1200:1211	arg1	location					1213:1220	the differential location	1196:1220	the differential location of a single sulfate at either C-6 of glucosamine or C-2 of uronic acid in the reducing disaccharide	1196:1320	The structural difference between the two hexasaccharides was defined as the differential location of a single sulfate at either C-6 of glucosamine or C-2 of uronic acid in the reducing disaccharide, resulting in a 200-fold difference in binding affinity for MCP-1.
27801570	7	12	theme	differential	1200:1211	arg1	difference					1138:1147	The structural difference	1123:1147	The structural difference between the two hexasaccharides	1123:1179	The structural difference between the two hexasaccharides was defined as the differential location of a single sulfate at either C-6 of glucosamine or C-2 of uronic acid in the reducing disaccharide, resulting in a 200-fold difference in binding affinity for MCP-1.
27801570	5	13	theme	hexasaccharide	966:979	arg1	conformation					981:992	a more compact protein:hexasaccharide conformation	943:992	a more compact protein:hexasaccharide conformation	943:992	A significant shift in arrival time distribution of close to 70 Å2 was observed, indicating a more compact protein:hexasaccharide conformation.
27801570	7	14	theme	acid	1288:1291	arg1	C-2					1274:1276	C-2	1274:1276	C-2 of uronic acid	1274:1291	The structural difference between the two hexasaccharides was defined as the differential location of a single sulfate at either C-6 of glucosamine or C-2 of uronic acid in the reducing disaccharide, resulting in a 200-fold difference in binding affinity for MCP-1.
27801570	7	14	theme	acid	1288:1291	arg1	C-6					1252:1254	C-6	1252:1254	C-6 of glucosamine	1252:1269	The structural difference between the two hexasaccharides was defined as the differential location of a single sulfate at either C-6 of glucosamine or C-2 of uronic acid in the reducing disaccharide, resulting in a 200-fold difference in binding affinity for MCP-1.
27801570	7	15	from	C-6	1252:1254	arg1	location					1213:1220	the differential location	1196:1220	the differential location of a single sulfate at either C-6 of glucosamine or C-2 of uronic acid in the reducing disaccharide	1196:1320	The structural difference between the two hexasaccharides was defined as the differential location of a single sulfate at either C-6 of glucosamine or C-2 of uronic acid in the reducing disaccharide, resulting in a 200-fold difference in binding affinity for MCP-1.
27801570	7	15	from	C-6	1252:1254	arg1	difference					1138:1147	The structural difference	1123:1147	The structural difference between the two hexasaccharides	1123:1179	The structural difference between the two hexasaccharides was defined as the differential location of a single sulfate at either C-6 of glucosamine or C-2 of uronic acid in the reducing disaccharide, resulting in a 200-fold difference in binding affinity for MCP-1.
27801570	7	15	from	C-6	1252:1254	arg1	sulfate					1234:1240	a single sulfate	1225:1240	a single sulfate at either C-6 of glucosamine or C-2 of uronic acid	1225:1291	The structural difference between the two hexasaccharides was defined as the differential location of a single sulfate at either C-6 of glucosamine or C-2 of uronic acid in the reducing disaccharide, resulting in a 200-fold difference in binding affinity for MCP-1.
27801570	3	16	theme	product	585:591	arg1	ions					593:596	unique MS/MS product ions	572:596	unique MS/MS product ions	572:596	The sequences of these two hexasaccharides were deduced from unique MS/MS product ions and HPLC compositional analysis.
27801570	4	17	theme	conformations	813:825	arg1	conformations					813:825	the two distinct conformations	796:825	the two distinct conformations known for MCP-1 dimers	796:848	Ion mobility mass spectrometry (IM-MS) showed that the two isolated oligosaccharides have different conformations and both displayed preferential binding for one of the two distinct conformations known for MCP-1 dimers.
27801570	4	17	theme	conformations	813:825	arg1	one					789:791	one	789:791	one	789:791	Ion mobility mass spectrometry (IM-MS) showed that the two isolated oligosaccharides have different conformations and both displayed preferential binding for one of the two distinct conformations known for MCP-1 dimers.
27801570	8	18	dep	simply	1508:1513	arg1	not					1504:1506	not	1504:1506	not	1504:1506	These data indicate sequence specificity for high affinity binding, supporting the view that sulfate position, and not simply the number of sulfates, is important for heparan sulfate protein binding.
27801570	3	19	theme	unique	572:577	arg1	ions					593:596	unique MS/MS product ions	572:596	unique MS/MS product ions	572:596	The sequences of these two hexasaccharides were deduced from unique MS/MS product ions and HPLC compositional analysis.
27801570	2	20	theme	binding	491:497	arg1	affinities					499:508	different binding affinities	481:508	different binding affinities	481:508	Here we demonstrate chromatographic isolation and purification of two heparin hexasaccharide isomers that interact with the oligomeric chemokine Monocyte Chemoattractant Protein-1 (MCP-1)/CCL2 with different binding affinities.
27801570	3	21	theme	compositional	607:619	arg1	analysis					621:628	HPLC compositional analysis	602:628	HPLC compositional analysis	602:628	The sequences of these two hexasaccharides were deduced from unique MS/MS product ions and HPLC compositional analysis.
27801570	8	22	theme	sequence	1409:1416	arg1	specificity					1418:1428	sequence specificity	1409:1428	sequence specificity	1409:1428	These data indicate sequence specificity for high affinity binding, supporting the view that sulfate position, and not simply the number of sulfates, is important for heparan sulfate protein binding.
27801570	7	23	theme	single	1227:1232	arg1	sulfate					1234:1240	a single sulfate	1225:1240	a single sulfate at either C-6 of glucosamine or C-2 of uronic acid	1225:1291	The structural difference between the two hexasaccharides was defined as the differential location of a single sulfate at either C-6 of glucosamine or C-2 of uronic acid in the reducing disaccharide, resulting in a 200-fold difference in binding affinity for MCP-1.
27801570	8	24	theme	sulfates	1529:1536	arg1	position					1490:1497	sulfate position	1482:1497	sulfate position	1482:1497	These data indicate sequence specificity for high affinity binding, supporting the view that sulfate position, and not simply the number of sulfates, is important for heparan sulfate protein binding.
27801570	8	24	theme	sulfates	1529:1536	arg1	important					1542:1550	important	1542:1550	important	1542:1550	These data indicate sequence specificity for high affinity binding, supporting the view that sulfate position, and not simply the number of sulfates, is important for heparan sulfate protein binding.
27801570	8	24	theme	sulfates	1529:1536	arg1	number					1519:1524	the number	1515:1524	the number of sulfates	1515:1536	These data indicate sequence specificity for high affinity binding, supporting the view that sulfate position, and not simply the number of sulfates, is important for heparan sulfate protein binding.
27801570	5	25	theme	arrival	874:880	arg1	distribution					887:898	arrival time distribution	874:898	arrival time distribution of close to 70 Å2	874:916	A significant shift in arrival time distribution of close to 70 Å2 was observed, indicating a more compact protein:hexasaccharide conformation.
27801570	8	26	theme	high	1434:1437	arg1	binding					1448:1454	high affinity binding	1434:1454	high affinity binding	1434:1454	These data indicate sequence specificity for high affinity binding, supporting the view that sulfate position, and not simply the number of sulfates, is important for heparan sulfate protein binding.
27801570	7	27	theme	glucosamine	1259:1269	arg1	C-2					1274:1276	C-2	1274:1276	C-2 of uronic acid	1274:1291	The structural difference between the two hexasaccharides was defined as the differential location of a single sulfate at either C-6 of glucosamine or C-2 of uronic acid in the reducing disaccharide, resulting in a 200-fold difference in binding affinity for MCP-1.
27801570	7	27	theme	glucosamine	1259:1269	arg1	C-6					1252:1254	C-6	1252:1254	C-6 of glucosamine	1252:1269	The structural difference between the two hexasaccharides was defined as the differential location of a single sulfate at either C-6 of glucosamine or C-2 of uronic acid in the reducing disaccharide, resulting in a 200-fold difference in binding affinity for MCP-1.
27801570	7	28	theme	reducing	1300:1307	arg1	disaccharide					1309:1320	the reducing disaccharide	1296:1320	the reducing disaccharide	1296:1320	The structural difference between the two hexasaccharides was defined as the differential location of a single sulfate at either C-6 of glucosamine or C-2 of uronic acid in the reducing disaccharide, resulting in a 200-fold difference in binding affinity for MCP-1.
27801570	4	29	theme	MCP-1	837:841	arg1	dimers					843:848	MCP-1 dimers	837:848	MCP-1 dimers	837:848	Ion mobility mass spectrometry (IM-MS) showed that the two isolated oligosaccharides have different conformations and both displayed preferential binding for one of the two distinct conformations known for MCP-1 dimers.
27801570	5	30	theme	compact	950:956	arg1	conformation					981:992	a more compact protein:hexasaccharide conformation	943:992	a more compact protein:hexasaccharide conformation	943:992	A significant shift in arrival time distribution of close to 70 Å2 was observed, indicating a more compact protein:hexasaccharide conformation.
27801570	4	31	theme	mass	644:647	arg1	IM-MS					663:667	IM-MS	663:667	IM-MS	663:667	Ion mobility mass spectrometry (IM-MS) showed that the two isolated oligosaccharides have different conformations and both displayed preferential binding for one of the two distinct conformations known for MCP-1 dimers.
27801570	4	31	theme	mass	644:647	arg1	spectrometry					649:660	Ion mobility mass spectrometry	631:660	Ion mobility mass spectrometry (IM-MS)	631:668	Ion mobility mass spectrometry (IM-MS) showed that the two isolated oligosaccharides have different conformations and both displayed preferential binding for one of the two distinct conformations known for MCP-1 dimers.
27801570	2	32	theme	isomers	376:382	arg1	purification					333:344	purification	333:344	purification	333:344	Here we demonstrate chromatographic isolation and purification of two heparin hexasaccharide isomers that interact with the oligomeric chemokine Monocyte Chemoattractant Protein-1 (MCP-1)/CCL2 with different binding affinities.
27801570	2	32	theme	isomers	376:382	arg1	isolation					319:327	chromatographic isolation	303:327	chromatographic isolation	303:327	Here we demonstrate chromatographic isolation and purification of two heparin hexasaccharide isomers that interact with the oligomeric chemokine Monocyte Chemoattractant Protein-1 (MCP-1)/CCL2 with different binding affinities.
27801570	4	33	theme	preferential	764:775	arg1	binding					777:783	preferential binding	764:783	preferential binding for one of the two distinct conformations known for MCP-1 dimers	764:848	Ion mobility mass spectrometry (IM-MS) showed that the two isolated oligosaccharides have different conformations and both displayed preferential binding for one of the two distinct conformations known for MCP-1 dimers.
27801570	0	34	theme	Isomeric	18:25	arg1	Oligosaccharides					35:50	Two Isomeric Heparin Oligosaccharides	14:50	Two Isomeric Heparin Oligosaccharides	14:50	Enrichment of Two Isomeric Heparin Oligosaccharides Exhibiting Different Affinities toward Monocyte Chemoattractant Protein-1.
27801570	4	35	theme	mobility	635:642	arg1	IM-MS					663:667	IM-MS	663:667	IM-MS	663:667	Ion mobility mass spectrometry (IM-MS) showed that the two isolated oligosaccharides have different conformations and both displayed preferential binding for one of the two distinct conformations known for MCP-1 dimers.
27801570	4	35	theme	mobility	635:642	arg1	spectrometry					649:660	Ion mobility mass spectrometry	631:660	Ion mobility mass spectrometry (IM-MS)	631:668	Ion mobility mass spectrometry (IM-MS) showed that the two isolated oligosaccharides have different conformations and both displayed preferential binding for one of the two distinct conformations known for MCP-1 dimers.
27801570	2	36	theme	hexasaccharide	361:374	arg1	isomers					376:382	two heparin hexasaccharide isomers	349:382	two heparin hexasaccharide isomers that interact with the oligomeric chemokine Monocyte Chemoattractant Protein-1 (MCP-1)/CCL2 with different binding affinities	349:508	Here we demonstrate chromatographic isolation and purification of two heparin hexasaccharide isomers that interact with the oligomeric chemokine Monocyte Chemoattractant Protein-1 (MCP-1)/CCL2 with different binding affinities.
27801570	1	37	theme	chemokine	236:244	arg1	gradients					246:254	chemokine gradients	236:254	chemokine gradients that guide cell migration	236:280	Chemokine-GAG interactions are crucial to facilitate chemokine immobilization, resulting in the formation of chemokine gradients that guide cell migration.
27801570	5	38	theme	Å2	915:916	arg1	distribution					887:898	arrival time distribution	874:898	arrival time distribution of close to 70 Å2	874:916	A significant shift in arrival time distribution of close to 70 Å2 was observed, indicating a more compact protein:hexasaccharide conformation.
27801570	6	39	theme	unbound	1049:1055	arg1	protein					1057:1063	bound and unbound protein	1039:1063	bound and unbound protein	1039:1063	Clear differences in the MS spectra between bound and unbound protein allowed calculation of Kd values from the resulting data.
27801570	8	40	theme	affinity	1439:1446	arg1	binding					1448:1454	high affinity binding	1434:1454	high affinity binding	1434:1454	These data indicate sequence specificity for high affinity binding, supporting the view that sulfate position, and not simply the number of sulfates, is important for heparan sulfate protein binding.
27801570	7	41	from	C-2	1274:1276	arg1	location					1213:1220	the differential location	1196:1220	the differential location of a single sulfate at either C-6 of glucosamine or C-2 of uronic acid in the reducing disaccharide	1196:1320	The structural difference between the two hexasaccharides was defined as the differential location of a single sulfate at either C-6 of glucosamine or C-2 of uronic acid in the reducing disaccharide, resulting in a 200-fold difference in binding affinity for MCP-1.
27801570	7	41	from	C-2	1274:1276	arg1	difference					1138:1147	The structural difference	1123:1147	The structural difference between the two hexasaccharides	1123:1179	The structural difference between the two hexasaccharides was defined as the differential location of a single sulfate at either C-6 of glucosamine or C-2 of uronic acid in the reducing disaccharide, resulting in a 200-fold difference in binding affinity for MCP-1.
27801570	7	41	from	C-2	1274:1276	arg1	sulfate					1234:1240	a single sulfate	1225:1240	a single sulfate at either C-6 of glucosamine or C-2 of uronic acid	1225:1291	The structural difference between the two hexasaccharides was defined as the differential location of a single sulfate at either C-6 of glucosamine or C-2 of uronic acid in the reducing disaccharide, resulting in a 200-fold difference in binding affinity for MCP-1.
27801570	2	42	theme	heparin	353:359	arg1	isomers					376:382	two heparin hexasaccharide isomers	349:382	two heparin hexasaccharide isomers that interact with the oligomeric chemokine Monocyte Chemoattractant Protein-1 (MCP-1)/CCL2 with different binding affinities	349:508	Here we demonstrate chromatographic isolation and purification of two heparin hexasaccharide isomers that interact with the oligomeric chemokine Monocyte Chemoattractant Protein-1 (MCP-1)/CCL2 with different binding affinities.
27801570	1	43	theme	gradients	246:254	arg1	formation					223:231	the formation	219:231	the formation of chemokine gradients that guide cell migration	219:280	Chemokine-GAG interactions are crucial to facilitate chemokine immobilization, resulting in the formation of chemokine gradients that guide cell migration.
27801570	0	44	theme	Oligosaccharides	35:50	arg1	Enrichment					0:9	Enrichment	0:9	Enrichment of Two Isomeric Heparin Oligosaccharides	0:50	Enrichment of Two Isomeric Heparin Oligosaccharides Exhibiting Different Affinities toward Monocyte Chemoattractant Protein-1.
27801570	7	45	theme	binding	1361:1367	arg1	affinity					1369:1376	binding affinity	1361:1376	binding affinity for MCP-1	1361:1386	The structural difference between the two hexasaccharides was defined as the differential location of a single sulfate at either C-6 of glucosamine or C-2 of uronic acid in the reducing disaccharide, resulting in a 200-fold difference in binding affinity for MCP-1.
27801570	5	46	theme	protein	958:964	arg1	conformation					981:992	a more compact protein:hexasaccharide conformation	943:992	a more compact protein:hexasaccharide conformation	943:992	A significant shift in arrival time distribution of close to 70 Å2 was observed, indicating a more compact protein:hexasaccharide conformation.
27801570	2	47	theme	different	481:489	arg1	affinities					499:508	different binding affinities	481:508	different binding affinities	481:508	Here we demonstrate chromatographic isolation and purification of two heparin hexasaccharide isomers that interact with the oligomeric chemokine Monocyte Chemoattractant Protein-1 (MCP-1)/CCL2 with different binding affinities.
27801570	0	48	theme	Heparin	27:33	arg1	Oligosaccharides					35:50	Two Isomeric Heparin Oligosaccharides	14:50	Two Isomeric Heparin Oligosaccharides	14:50	Enrichment of Two Isomeric Heparin Oligosaccharides Exhibiting Different Affinities toward Monocyte Chemoattractant Protein-1.
27801570	6	49	theme	bound	1039:1043	arg1	protein					1057:1063	bound and unbound protein	1039:1063	bound and unbound protein	1039:1063	Clear differences in the MS spectra between bound and unbound protein allowed calculation of Kd values from the resulting data.
27801570	5	50	theme	time	882:885	arg1	distribution					887:898	arrival time distribution	874:898	arrival time distribution of close to 70 Å2	874:916	A significant shift in arrival time distribution of close to 70 Å2 was observed, indicating a more compact protein:hexasaccharide conformation.
27801570	4	51	contain	have	716:719	arg2	conformations					731:743	different conformations	721:743	different conformations	721:743	Ion mobility mass spectrometry (IM-MS) showed that the two isolated oligosaccharides have different conformations and both displayed preferential binding for one of the two distinct conformations known for MCP-1 dimers.
27801570	4	51	contain	have	716:719	arg1	oligosaccharides					699:714	the two isolated oligosaccharides	682:714	the two isolated oligosaccharides	682:714	Ion mobility mass spectrometry (IM-MS) showed that the two isolated oligosaccharides have different conformations and both displayed preferential binding for one of the two distinct conformations known for MCP-1 dimers.
27801570	0	52	theme	Different	63:71	arg1	Affinities					73:82	Different Affinities	63:82	Different Affinities	63:82	Enrichment of Two Isomeric Heparin Oligosaccharides Exhibiting Different Affinities toward Monocyte Chemoattractant Protein-1.
27801570	7	53	from	location	1213:1220	arg1	C-2					1274:1276	C-2	1274:1276	C-2 of uronic acid	1274:1291	The structural difference between the two hexasaccharides was defined as the differential location of a single sulfate at either C-6 of glucosamine or C-2 of uronic acid in the reducing disaccharide, resulting in a 200-fold difference in binding affinity for MCP-1.
27801570	7	53	from	location	1213:1220	arg1	C-6					1252:1254	C-6	1252:1254	C-6 of glucosamine	1252:1269	The structural difference between the two hexasaccharides was defined as the differential location of a single sulfate at either C-6 of glucosamine or C-2 of uronic acid in the reducing disaccharide, resulting in a 200-fold difference in binding affinity for MCP-1.
27801570	7	53	from	location	1213:1220	arg1	disaccharide					1309:1320	the reducing disaccharide	1296:1320	the reducing disaccharide	1296:1320	The structural difference between the two hexasaccharides was defined as the differential location of a single sulfate at either C-6 of glucosamine or C-2 of uronic acid in the reducing disaccharide, resulting in a 200-fold difference in binding affinity for MCP-1.
27801570	7	54	theme	structural	1127:1136	arg1	location					1213:1220	the differential location	1196:1220	the differential location of a single sulfate at either C-6 of glucosamine or C-2 of uronic acid in the reducing disaccharide	1196:1320	The structural difference between the two hexasaccharides was defined as the differential location of a single sulfate at either C-6 of glucosamine or C-2 of uronic acid in the reducing disaccharide, resulting in a 200-fold difference in binding affinity for MCP-1.
27801570	7	54	theme	structural	1127:1136	arg1	difference					1138:1147	The structural difference	1123:1147	The structural difference between the two hexasaccharides	1123:1179	The structural difference between the two hexasaccharides was defined as the differential location of a single sulfate at either C-6 of glucosamine or C-2 of uronic acid in the reducing disaccharide, resulting in a 200-fold difference in binding affinity for MCP-1.
27801570	2	55	theme	oligomeric	407:416	arg1	MCP-1					464:468	MCP-1	464:468	MCP-1	464:468	Here we demonstrate chromatographic isolation and purification of two heparin hexasaccharide isomers that interact with the oligomeric chemokine Monocyte Chemoattractant Protein-1 (MCP-1)/CCL2 with different binding affinities.
27801570	2	55	theme	oligomeric	407:416	arg1	Protein-1					453:461	oligomeric chemokine Monocyte Chemoattractant Protein-1	407:461	the oligomeric chemokine Monocyte Chemoattractant Protein-1 (MCP-1)/CCL2 with different binding affinities	403:508	Here we demonstrate chromatographic isolation and purification of two heparin hexasaccharide isomers that interact with the oligomeric chemokine Monocyte Chemoattractant Protein-1 (MCP-1)/CCL2 with different binding affinities.
27801570	7	56	theme	sulfate	1234:1240	arg1	location					1213:1220	the differential location	1196:1220	the differential location of a single sulfate at either C-6 of glucosamine or C-2 of uronic acid in the reducing disaccharide	1196:1320	The structural difference between the two hexasaccharides was defined as the differential location of a single sulfate at either C-6 of glucosamine or C-2 of uronic acid in the reducing disaccharide, resulting in a 200-fold difference in binding affinity for MCP-1.
27801570	7	56	theme	sulfate	1234:1240	arg1	difference					1138:1147	The structural difference	1123:1147	The structural difference between the two hexasaccharides	1123:1179	The structural difference between the two hexasaccharides was defined as the differential location of a single sulfate at either C-6 of glucosamine or C-2 of uronic acid in the reducing disaccharide, resulting in a 200-fold difference in binding affinity for MCP-1.
27801570	4	57	theme	Ion	631:633	arg1	IM-MS					663:667	IM-MS	663:667	IM-MS	663:667	Ion mobility mass spectrometry (IM-MS) showed that the two isolated oligosaccharides have different conformations and both displayed preferential binding for one of the two distinct conformations known for MCP-1 dimers.
27801570	4	57	theme	Ion	631:633	arg1	spectrometry					649:660	Ion mobility mass spectrometry	631:660	Ion mobility mass spectrometry (IM-MS)	631:668	Ion mobility mass spectrometry (IM-MS) showed that the two isolated oligosaccharides have different conformations and both displayed preferential binding for one of the two distinct conformations known for MCP-1 dimers.
27801570	5	58	from	shift	865:869	arg1	distribution					887:898	arrival time distribution	874:898	arrival time distribution of close to 70 Å2	874:916	A significant shift in arrival time distribution of close to 70 Å2 was observed, indicating a more compact protein:hexasaccharide conformation.
27801570	8	59	theme	sulfate	1482:1488	arg1	position					1490:1497	sulfate position	1482:1497	sulfate position	1482:1497	These data indicate sequence specificity for high affinity binding, supporting the view that sulfate position, and not simply the number of sulfates, is important for heparan sulfate protein binding.
27801570	3	60	theme	MS/MS	579:583	arg1	ions					593:596	unique MS/MS product ions	572:596	unique MS/MS product ions	572:596	The sequences of these two hexasaccharides were deduced from unique MS/MS product ions and HPLC compositional analysis.
27801570	6	61	theme	MS	1020:1021	arg1	spectra					1023:1029	the MS spectra	1016:1029	the MS spectra	1016:1029	Clear differences in the MS spectra between bound and unbound protein allowed calculation of Kd values from the resulting data.
27801570	4	62	theme	distinct	804:811	arg1	conformations					813:825	the two distinct conformations	796:825	the two distinct conformations known for MCP-1 dimers	796:848	Ion mobility mass spectrometry (IM-MS) showed that the two isolated oligosaccharides have different conformations and both displayed preferential binding for one of the two distinct conformations known for MCP-1 dimers.
27801570	1	63	theme	chemokine	180:188	arg1	immobilization					190:203	chemokine immobilization	180:203	chemokine immobilization	180:203	Chemokine-GAG interactions are crucial to facilitate chemokine immobilization, resulting in the formation of chemokine gradients that guide cell migration.
27801570	7	64	theme	200-fold	1338:1345	arg1	difference					1347:1356	a 200-fold difference	1336:1356	a 200-fold difference in binding affinity for MCP-1	1336:1386	The structural difference between the two hexasaccharides was defined as the differential location of a single sulfate at either C-6 of glucosamine or C-2 of uronic acid in the reducing disaccharide, resulting in a 200-fold difference in binding affinity for MCP-1.
27801570	1	65	theme	Chemokine-GAG	127:139	arg1	interactions					141:152	Chemokine-GAG interactions	127:152	Chemokine-GAG interactions	127:152	Chemokine-GAG interactions are crucial to facilitate chemokine immobilization, resulting in the formation of chemokine gradients that guide cell migration.
27801570	1	66	theme	cell	267:270	arg1	migration					272:280	cell migration	267:280	cell migration	267:280	Chemokine-GAG interactions are crucial to facilitate chemokine immobilization, resulting in the formation of chemokine gradients that guide cell migration.
27801570	2	67	theme	chromatographic	303:317	arg1	isolation					319:327	chromatographic isolation	303:327	chromatographic isolation	303:327	Here we demonstrate chromatographic isolation and purification of two heparin hexasaccharide isomers that interact with the oligomeric chemokine Monocyte Chemoattractant Protein-1 (MCP-1)/CCL2 with different binding affinities.
27801570	3	68	theme	HPLC	602:605	arg1	analysis					621:628	HPLC compositional analysis	602:628	HPLC compositional analysis	602:628	The sequences of these two hexasaccharides were deduced from unique MS/MS product ions and HPLC compositional analysis.
27801570	6	69	theme	resulting	1107:1115	arg1	data					1117:1120	the resulting data	1103:1120	the resulting data	1103:1120	Clear differences in the MS spectra between bound and unbound protein allowed calculation of Kd values from the resulting data.
27801570	6	70	from	differences	1001:1011	arg1	spectra					1023:1029	the MS spectra	1016:1029	the MS spectra	1016:1029	Clear differences in the MS spectra between bound and unbound protein allowed calculation of Kd values from the resulting data.
27801570	7	71	from	difference	1347:1356	arg1	affinity					1369:1376	binding affinity	1361:1376	binding affinity for MCP-1	1361:1386	The structural difference between the two hexasaccharides was defined as the differential location of a single sulfate at either C-6 of glucosamine or C-2 of uronic acid in the reducing disaccharide, resulting in a 200-fold difference in binding affinity for MCP-1.
27801570	3	72	theme	hexasaccharides	538:552	arg1	sequences					515:523	The sequences	511:523	The sequences of these two hexasaccharides	511:552	The sequences of these two hexasaccharides were deduced from unique MS/MS product ions and HPLC compositional analysis.
27801570	2	73	theme	Protein-1	453:461	arg1	/CCL2					470:474	the oligomeric chemokine Monocyte Chemoattractant Protein-1 (MCP-1)/CCL2	403:474	the oligomeric chemokine Monocyte Chemoattractant Protein-1 (MCP-1)/CCL2 with different binding affinities	403:508	Here we demonstrate chromatographic isolation and purification of two heparin hexasaccharide isomers that interact with the oligomeric chemokine Monocyte Chemoattractant Protein-1 (MCP-1)/CCL2 with different binding affinities.
27801570	5	74	theme	significant	853:863	arg1	shift					865:869	A significant shift	851:869	A significant shift in arrival time distribution of close to 70 Å2	851:916	A significant shift in arrival time distribution of close to 70 Å2 was observed, indicating a more compact protein:hexasaccharide conformation.
27801570	8	75	theme	sulfate	1564:1570	arg1	binding					1580:1586	heparan sulfate protein binding	1556:1586	heparan sulfate protein binding	1556:1586	These data indicate sequence specificity for high affinity binding, supporting the view that sulfate position, and not simply the number of sulfates, is important for heparan sulfate protein binding.
27801570	4	76	theme	different	721:729	arg1	conformations					731:743	different conformations	721:743	different conformations	721:743	Ion mobility mass spectrometry (IM-MS) showed that the two isolated oligosaccharides have different conformations and both displayed preferential binding for one of the two distinct conformations known for MCP-1 dimers.
27801570	2	77	theme	Chemoattractant	437:451	arg1	MCP-1					464:468	MCP-1	464:468	MCP-1	464:468	Here we demonstrate chromatographic isolation and purification of two heparin hexasaccharide isomers that interact with the oligomeric chemokine Monocyte Chemoattractant Protein-1 (MCP-1)/CCL2 with different binding affinities.
27801570	2	77	theme	Chemoattractant	437:451	arg1	Protein-1					453:461	oligomeric chemokine Monocyte Chemoattractant Protein-1	407:461	the oligomeric chemokine Monocyte Chemoattractant Protein-1 (MCP-1)/CCL2 with different binding affinities	403:508	Here we demonstrate chromatographic isolation and purification of two heparin hexasaccharide isomers that interact with the oligomeric chemokine Monocyte Chemoattractant Protein-1 (MCP-1)/CCL2 with different binding affinities.
27801570	4	78	theme	isolated	690:697	arg1	oligosaccharides					699:714	the two isolated oligosaccharides	682:714	the two isolated oligosaccharides	682:714	Ion mobility mass spectrometry (IM-MS) showed that the two isolated oligosaccharides have different conformations and both displayed preferential binding for one of the two distinct conformations known for MCP-1 dimers.
27744242	3	0	theme	hemicellulases	323:336	arg1	titers					298:303	appreciable titers	286:303	appreciable titers of cellulases and hemicellulases	286:336	Scytalidium thermophilum/Humicola insolens), a thermophilic fungus, is being reported to produce appreciable titers of cellulases and hemicellulases during shake flask culturing on cellulose/wheat-bran/rice straw based production medium.
27744242	5	1	theme	glycosyl	691:698	arg1	hydrolases					700:709	62 glycosyl hydrolases	688:709	62 glycosyl hydrolases belonging to 30 different families	688:744	Mass spectrometry analysis of secretome (Q-TOF LC/MS) revealed a total of 240 proteins with 92 CAZymes of which 62 glycosyl hydrolases belonging to 30 different families were present.
27744242	5	2	theme	secretome	606:614	arg1	analysis					594:601	Mass spectrometry analysis	576:601	Mass spectrometry analysis of secretome (Q-TOF LC/MS)	576:628	Mass spectrometry analysis of secretome (Q-TOF LC/MS) revealed a total of 240 proteins with 92 CAZymes of which 62 glycosyl hydrolases belonging to 30 different families were present.
27744242	2	3	dep	thermophilus	170:181	arg1	Syn					184:186	Syn	184:186	Syn	184:186	Mycothermus thermophilus (Syn.
27744242	3	4	theme	thermophilic	236:247	arg1	thermophilum/Humicola					201:221	Scytalidium thermophilum/Humicola	189:221	Scytalidium thermophilum/Humicola insolens)	189:231	Scytalidium thermophilum/Humicola insolens), a thermophilic fungus, is being reported to produce appreciable titers of cellulases and hemicellulases during shake flask culturing on cellulose/wheat-bran/rice straw based production medium.
27744242	3	4	theme	thermophilic	236:247	arg1	fungus					249:254	a thermophilic fungus	234:254	a thermophilic fungus	234:254	Scytalidium thermophilum/Humicola insolens), a thermophilic fungus, is being reported to produce appreciable titers of cellulases and hemicellulases during shake flask culturing on cellulose/wheat-bran/rice straw based production medium.
27744242	4	5	theme	sequential	431:440	arg1	profile					470:476	The sequential and differential expression profile	427:476	The sequential and differential expression profile of endoglucanases, β-glucosidases, cellobiohydrolases and xylanases using zymography	427:561	The sequential and differential expression profile of endoglucanases, β-glucosidases, cellobiohydrolases and xylanases using zymography was studied.
27744242	3	6	dep	thermophilum/Humicola	201:221	arg1	insolens					223:230	insolens	223:230	Scytalidium thermophilum/Humicola insolens)	189:231	Scytalidium thermophilum/Humicola insolens), a thermophilic fungus, is being reported to produce appreciable titers of cellulases and hemicellulases during shake flask culturing on cellulose/wheat-bran/rice straw based production medium.
27744242	4	7	theme	β-glucosidases	497:510	arg1	profile					470:476	The sequential and differential expression profile	427:476	The sequential and differential expression profile of endoglucanases, β-glucosidases, cellobiohydrolases and xylanases using zymography	427:561	The sequential and differential expression profile of endoglucanases, β-glucosidases, cellobiohydrolases and xylanases using zymography was studied.
27744242	3	8	theme	cellulose/wheat-bran/rice	370:394	arg1	production					408:417	cellulose/wheat-bran/rice straw based production	370:417	cellulose/wheat-bran/rice straw based production medium	370:424	Scytalidium thermophilum/Humicola insolens), a thermophilic fungus, is being reported to produce appreciable titers of cellulases and hemicellulases during shake flask culturing on cellulose/wheat-bran/rice straw based production medium.
27744242	5	9	theme	proteins	654:661	arg1	total					641:645	a total	639:645	a total of 240 proteins with 92 CAZymes of which 62 glycosyl hydrolases belonging to 30 different families were present	639:757	Mass spectrometry analysis of secretome (Q-TOF LC/MS) revealed a total of 240 proteins with 92 CAZymes of which 62 glycosyl hydrolases belonging to 30 different families were present.
27744242	1	10	from	Repertoire	58:67	arg1	secretome					138:146	the secretome	134:146	the secretome	134:146	Scytalidium thermophilum): Repertoire of a diverse array of efficient cellulases and hemicellulases in the secretome revealed.
27744242	3	11	theme	straw	396:400	arg1	production					408:417	cellulose/wheat-bran/rice straw based production	370:417	cellulose/wheat-bran/rice straw based production medium	370:424	Scytalidium thermophilum/Humicola insolens), a thermophilic fungus, is being reported to produce appreciable titers of cellulases and hemicellulases during shake flask culturing on cellulose/wheat-bran/rice straw based production medium.
27744242	4	12	theme	xylanases	536:544	arg1	profile					470:476	The sequential and differential expression profile	427:476	The sequential and differential expression profile of endoglucanases, β-glucosidases, cellobiohydrolases and xylanases using zymography	427:561	The sequential and differential expression profile of endoglucanases, β-glucosidases, cellobiohydrolases and xylanases using zymography was studied.
27744242	7	13	theme	genomic	1063:1069	arg1	database					1071:1078	genomic database	1063:1078	genomic database of the culture	1063:1093	In addition, carbohydrate esterases, polysaccharide lyases, auxiliary activity and a variety of carbohydrate binding modules (CBM) were identified using genomic database of the culture indicating to an elaborate genetic potential of this strain for hydrolysis of lignocellulosics.
27744242	4	14	theme	differential	446:457	arg1	profile					470:476	The sequential and differential expression profile	427:476	The sequential and differential expression profile of endoglucanases, β-glucosidases, cellobiohydrolases and xylanases using zymography	427:561	The sequential and differential expression profile of endoglucanases, β-glucosidases, cellobiohydrolases and xylanases using zymography was studied.
27744242	5	15	theme	CAZymes	671:677	arg1	present					751:757	present	751:757	present	751:757	Mass spectrometry analysis of secretome (Q-TOF LC/MS) revealed a total of 240 proteins with 92 CAZymes of which 62 glycosyl hydrolases belonging to 30 different families were present.
27744242	7	16	theme	genetic	1122:1128	arg1	potential					1130:1138	genetic potential	1122:1138	genetic potential of this strain for hydrolysis of lignocellulosics	1122:1188	In addition, carbohydrate esterases, polysaccharide lyases, auxiliary activity and a variety of carbohydrate binding modules (CBM) were identified using genomic database of the culture indicating to an elaborate genetic potential of this strain for hydrolysis of lignocellulosics.
27744242	7	17	theme	lignocellulosics	1173:1188	arg1	hydrolysis					1159:1168	hydrolysis	1159:1168	hydrolysis of lignocellulosics	1159:1188	In addition, carbohydrate esterases, polysaccharide lyases, auxiliary activity and a variety of carbohydrate binding modules (CBM) were identified using genomic database of the culture indicating to an elaborate genetic potential of this strain for hydrolysis of lignocellulosics.
27744242	1	18	theme	diverse	74:80	arg1	array					82:86	a diverse array	72:86	a diverse array of efficient cellulases and hemicellulases	72:129	Scytalidium thermophilum): Repertoire of a diverse array of efficient cellulases and hemicellulases in the secretome revealed.
27744242	5	19	theme	Mass	576:579	arg1	analysis					594:601	Mass spectrometry analysis	576:601	Mass spectrometry analysis of secretome (Q-TOF LC/MS)	576:628	Mass spectrometry analysis of secretome (Q-TOF LC/MS) revealed a total of 240 proteins with 92 CAZymes of which 62 glycosyl hydrolases belonging to 30 different families were present.
27744242	0	20	theme	Mycothermus	0:10	arg1	thermophilus					12:23	Mycothermus thermophilus	0:23	Mycothermus thermophilus (Syn.	0:29	Mycothermus thermophilus (Syn.
27744242	1	21	theme	array	82:86	arg1	array					82:86	a diverse array	72:86	a diverse array of efficient cellulases and hemicellulases	72:129	Scytalidium thermophilum): Repertoire of a diverse array of efficient cellulases and hemicellulases in the secretome revealed.
27744242	1	21	theme	array	82:86	arg1	Repertoire					58:67	Scytalidium thermophilum): Repertoire	31:67	Scytalidium thermophilum): Repertoire of a diverse array of efficient cellulases and hemicellulases in the secretome	31:146	Scytalidium thermophilum): Repertoire of a diverse array of efficient cellulases and hemicellulases in the secretome revealed.
27744242	7	22	dep	elaborate	1112:1120	arg1	an					1109:1110	an	1109:1110	an	1109:1110	In addition, carbohydrate esterases, polysaccharide lyases, auxiliary activity and a variety of carbohydrate binding modules (CBM) were identified using genomic database of the culture indicating to an elaborate genetic potential of this strain for hydrolysis of lignocellulosics.
27744242	3	23	theme	based	402:406	arg1	production					408:417	cellulose/wheat-bran/rice straw based production	370:417	cellulose/wheat-bran/rice straw based production medium	370:424	Scytalidium thermophilum/Humicola insolens), a thermophilic fungus, is being reported to produce appreciable titers of cellulases and hemicellulases during shake flask culturing on cellulose/wheat-bran/rice straw based production medium.
27744242	7	24	theme	modules	1027:1033	arg1	variety					995:1001	a variety	993:1001	a variety of carbohydrate binding modules (CBM)	993:1039	In addition, carbohydrate esterases, polysaccharide lyases, auxiliary activity and a variety of carbohydrate binding modules (CBM) were identified using genomic database of the culture indicating to an elaborate genetic potential of this strain for hydrolysis of lignocellulosics.
27744242	7	24	theme	modules	1027:1033	arg1	CBM					1036:1038	CBM	1036:1038	CBM	1036:1038	In addition, carbohydrate esterases, polysaccharide lyases, auxiliary activity and a variety of carbohydrate binding modules (CBM) were identified using genomic database of the culture indicating to an elaborate genetic potential of this strain for hydrolysis of lignocellulosics.
27744242	7	24	theme	modules	1027:1033	arg1	lyases					962:967	polysaccharide lyases	947:967	polysaccharide lyases	947:967	In addition, carbohydrate esterases, polysaccharide lyases, auxiliary activity and a variety of carbohydrate binding modules (CBM) were identified using genomic database of the culture indicating to an elaborate genetic potential of this strain for hydrolysis of lignocellulosics.
27744242	7	24	theme	modules	1027:1033	arg1	activity					980:987	auxiliary activity	970:987	auxiliary activity	970:987	In addition, carbohydrate esterases, polysaccharide lyases, auxiliary activity and a variety of carbohydrate binding modules (CBM) were identified using genomic database of the culture indicating to an elaborate genetic potential of this strain for hydrolysis of lignocellulosics.
27744242	7	24	theme	modules	1027:1033	arg1	modules					1027:1033	carbohydrate binding modules	1006:1033	carbohydrate binding modules (CBM)	1006:1039	In addition, carbohydrate esterases, polysaccharide lyases, auxiliary activity and a variety of carbohydrate binding modules (CBM) were identified using genomic database of the culture indicating to an elaborate genetic potential of this strain for hydrolysis of lignocellulosics.
27744242	7	24	theme	modules	1027:1033	arg1	esterases					936:944	carbohydrate esterases	923:944	carbohydrate esterases	923:944	In addition, carbohydrate esterases, polysaccharide lyases, auxiliary activity and a variety of carbohydrate binding modules (CBM) were identified using genomic database of the culture indicating to an elaborate genetic potential of this strain for hydrolysis of lignocellulosics.
27744242	3	25	theme	Scytalidium	189:199	arg1	thermophilum/Humicola					201:221	Scytalidium thermophilum/Humicola	189:221	Scytalidium thermophilum/Humicola insolens)	189:231	Scytalidium thermophilum/Humicola insolens), a thermophilic fungus, is being reported to produce appreciable titers of cellulases and hemicellulases during shake flask culturing on cellulose/wheat-bran/rice straw based production medium.
27744242	3	25	theme	Scytalidium	189:199	arg1	fungus					249:254	a thermophilic fungus	234:254	a thermophilic fungus	234:254	Scytalidium thermophilum/Humicola insolens), a thermophilic fungus, is being reported to produce appreciable titers of cellulases and hemicellulases during shake flask culturing on cellulose/wheat-bran/rice straw based production medium.
27744242	3	26	theme	production	408:417	arg1	medium					419:424	cellulose/wheat-bran/rice straw based production medium	370:424	cellulose/wheat-bran/rice straw based production medium	370:424	Scytalidium thermophilum/Humicola insolens), a thermophilic fungus, is being reported to produce appreciable titers of cellulases and hemicellulases during shake flask culturing on cellulose/wheat-bran/rice straw based production medium.
27744242	3	27	theme	appreciable	286:296	arg1	titers					298:303	appreciable titers	286:303	appreciable titers of cellulases and hemicellulases	286:336	Scytalidium thermophilum/Humicola insolens), a thermophilic fungus, is being reported to produce appreciable titers of cellulases and hemicellulases during shake flask culturing on cellulose/wheat-bran/rice straw based production medium.
27744242	3	28	from	culturing	357:365	arg1	medium					419:424	cellulose/wheat-bran/rice straw based production medium	370:424	cellulose/wheat-bran/rice straw based production medium	370:424	Scytalidium thermophilum/Humicola insolens), a thermophilic fungus, is being reported to produce appreciable titers of cellulases and hemicellulases during shake flask culturing on cellulose/wheat-bran/rice straw based production medium.
27744242	1	29	theme	efficient	91:99	arg1	cellulases					101:110	efficient cellulases	91:110	efficient cellulases	91:110	Scytalidium thermophilum): Repertoire of a diverse array of efficient cellulases and hemicellulases in the secretome revealed.
27744242	7	30	theme	carbohydrate	1006:1017	arg1	CBM					1036:1038	CBM	1036:1038	CBM	1036:1038	In addition, carbohydrate esterases, polysaccharide lyases, auxiliary activity and a variety of carbohydrate binding modules (CBM) were identified using genomic database of the culture indicating to an elaborate genetic potential of this strain for hydrolysis of lignocellulosics.
27744242	7	30	theme	carbohydrate	1006:1017	arg1	modules					1027:1033	carbohydrate binding modules	1006:1033	carbohydrate binding modules (CBM)	1006:1039	In addition, carbohydrate esterases, polysaccharide lyases, auxiliary activity and a variety of carbohydrate binding modules (CBM) were identified using genomic database of the culture indicating to an elaborate genetic potential of this strain for hydrolysis of lignocellulosics.
27744242	8	31	theme	treated	1240:1246	arg1	straw					1253:1257	alkali treated rice straw	1233:1257	alkali treated rice straw	1233:1257	The cellulases from the strain hydrolyzed alkali treated rice straw and bagasse into fermentable sugars efficiently.
27744242	7	32	theme	binding	1019:1025	arg1	CBM					1036:1038	CBM	1036:1038	CBM	1036:1038	In addition, carbohydrate esterases, polysaccharide lyases, auxiliary activity and a variety of carbohydrate binding modules (CBM) were identified using genomic database of the culture indicating to an elaborate genetic potential of this strain for hydrolysis of lignocellulosics.
27744242	7	32	theme	binding	1019:1025	arg1	modules					1027:1033	carbohydrate binding modules	1006:1033	carbohydrate binding modules (CBM)	1006:1039	In addition, carbohydrate esterases, polysaccharide lyases, auxiliary activity and a variety of carbohydrate binding modules (CBM) were identified using genomic database of the culture indicating to an elaborate genetic potential of this strain for hydrolysis of lignocellulosics.
27744242	4	33	theme	cellobiohydrolases	513:530	arg1	profile					470:476	The sequential and differential expression profile	427:476	The sequential and differential expression profile of endoglucanases, β-glucosidases, cellobiohydrolases and xylanases using zymography	427:561	The sequential and differential expression profile of endoglucanases, β-glucosidases, cellobiohydrolases and xylanases using zymography was studied.
27744242	7	34	theme	auxiliary	970:978	arg1	activity					980:987	auxiliary activity	970:987	auxiliary activity	970:987	In addition, carbohydrate esterases, polysaccharide lyases, auxiliary activity and a variety of carbohydrate binding modules (CBM) were identified using genomic database of the culture indicating to an elaborate genetic potential of this strain for hydrolysis of lignocellulosics.
27744242	1	35	dep	Scytalidium	31:41	arg1	thermophilum					43:54	thermophilum	43:54	thermophilum	43:54	Scytalidium thermophilum): Repertoire of a diverse array of efficient cellulases and hemicellulases in the secretome revealed.
27744242	7	36	theme	strain	1148:1153	arg1	potential					1130:1138	genetic potential	1122:1138	genetic potential of this strain for hydrolysis of lignocellulosics	1122:1188	In addition, carbohydrate esterases, polysaccharide lyases, auxiliary activity and a variety of carbohydrate binding modules (CBM) were identified using genomic database of the culture indicating to an elaborate genetic potential of this strain for hydrolysis of lignocellulosics.
27744242	3	37	theme	cellulases	308:317	arg1	titers					298:303	appreciable titers	286:303	appreciable titers of cellulases and hemicellulases	286:336	Scytalidium thermophilum/Humicola insolens), a thermophilic fungus, is being reported to produce appreciable titers of cellulases and hemicellulases during shake flask culturing on cellulose/wheat-bran/rice straw based production medium.
27744242	2	38	theme	Mycothermus	158:168	arg1	thermophilus					170:181	Mycothermus thermophilus	158:181	Mycothermus thermophilus (Syn.	158:187	Mycothermus thermophilus (Syn.
27744242	5	39	theme	Q-TOF	617:621	arg1	secretome					606:614	secretome	606:614	secretome (Q-TOF LC/MS)	606:628	Mass spectrometry analysis of secretome (Q-TOF LC/MS) revealed a total of 240 proteins with 92 CAZymes of which 62 glycosyl hydrolases belonging to 30 different families were present.
27744242	5	39	theme	Q-TOF	617:621	arg1	LC/MS					623:627	Q-TOF LC/MS	617:627	Q-TOF LC/MS	617:627	Mass spectrometry analysis of secretome (Q-TOF LC/MS) revealed a total of 240 proteins with 92 CAZymes of which 62 glycosyl hydrolases belonging to 30 different families were present.
27744242	1	40	theme	cellulases	101:110	arg1	array					82:86	a diverse array	72:86	a diverse array of efficient cellulases and hemicellulases	72:129	Scytalidium thermophilum): Repertoire of a diverse array of efficient cellulases and hemicellulases in the secretome revealed.
27744242	7	41	theme	culture	1087:1093	arg1	database					1071:1078	genomic database	1063:1078	genomic database of the culture	1063:1093	In addition, carbohydrate esterases, polysaccharide lyases, auxiliary activity and a variety of carbohydrate binding modules (CBM) were identified using genomic database of the culture indicating to an elaborate genetic potential of this strain for hydrolysis of lignocellulosics.
27744242	5	42	theme	different	727:735	arg1	families					737:744	30 different families	724:744	30 different families	724:744	Mass spectrometry analysis of secretome (Q-TOF LC/MS) revealed a total of 240 proteins with 92 CAZymes of which 62 glycosyl hydrolases belonging to 30 different families were present.
27744242	6	43	theme	β	790:790	arg1	Cellobiohydrolase					760:776	Cellobiohydrolase I	760:778	Cellobiohydrolase I (17.42%)	760:787	Cellobiohydrolase I (17.42%), β glucosidase (8.69%), endoglucanase (6.2%), xylanase (4.16%) and AA9 (3.95%) were the major proteins in the secretome.
27744242	6	43	theme	β	790:790	arg1	xylanase					835:842	xylanase	835:842	xylanase (4.16%)	835:850	Cellobiohydrolase I (17.42%), β glucosidase (8.69%), endoglucanase (6.2%), xylanase (4.16%) and AA9 (3.95%) were the major proteins in the secretome.
27744242	6	43	theme	β	790:790	arg1	AA9					856:858	AA9	856:858	AA9 (3.95%)	856:866	Cellobiohydrolase I (17.42%), β glucosidase (8.69%), endoglucanase (6.2%), xylanase (4.16%) and AA9 (3.95%) were the major proteins in the secretome.
27744242	6	43	theme	β	790:790	arg1	glucosidase					792:802	β glucosidase	790:802	β glucosidase (8.69%)	790:810	Cellobiohydrolase I (17.42%), β glucosidase (8.69%), endoglucanase (6.2%), xylanase (4.16%) and AA9 (3.95%) were the major proteins in the secretome.
27744242	6	43	theme	β	790:790	arg1	endoglucanase					813:825	endoglucanase	813:825	endoglucanase (6.2%)	813:832	Cellobiohydrolase I (17.42%), β glucosidase (8.69%), endoglucanase (6.2%), xylanase (4.16%) and AA9 (3.95%) were the major proteins in the secretome.
27744242	6	43	theme	β	790:790	arg1	proteins					883:890	the major proteins	873:890	the major proteins in the secretome	873:907	Cellobiohydrolase I (17.42%), β glucosidase (8.69%), endoglucanase (6.2%), xylanase (4.16%) and AA9 (3.95%) were the major proteins in the secretome.
27744242	6	43	theme	β	790:790	arg1	%					809:809	8.69%	805:809	8.69%	805:809	Cellobiohydrolase I (17.42%), β glucosidase (8.69%), endoglucanase (6.2%), xylanase (4.16%) and AA9 (3.95%) were the major proteins in the secretome.
27744242	8	44	theme	rice	1248:1251	arg1	straw					1253:1257	alkali treated rice straw	1233:1257	alkali treated rice straw	1233:1257	The cellulases from the strain hydrolyzed alkali treated rice straw and bagasse into fermentable sugars efficiently.
27744242	5	45	with	proteins	654:661	arg1	CAZymes					671:677	92 CAZymes	668:677	92 CAZymes of which 62 glycosyl hydrolases belonging to 30 different families were present	668:757	Mass spectrometry analysis of secretome (Q-TOF LC/MS) revealed a total of 240 proteins with 92 CAZymes of which 62 glycosyl hydrolases belonging to 30 different families were present.
27744242	4	46	theme	expression	459:468	arg1	profile					470:476	The sequential and differential expression profile	427:476	The sequential and differential expression profile of endoglucanases, β-glucosidases, cellobiohydrolases and xylanases using zymography	427:561	The sequential and differential expression profile of endoglucanases, β-glucosidases, cellobiohydrolases and xylanases using zymography was studied.
27744242	1	47	theme	hemicellulases	116:129	arg1	array					82:86	a diverse array	72:86	a diverse array of efficient cellulases and hemicellulases	72:129	Scytalidium thermophilum): Repertoire of a diverse array of efficient cellulases and hemicellulases in the secretome revealed.
27744242	8	48	from	strain	1215:1220	arg1	cellulases					1195:1204	The cellulases	1191:1204	The cellulases from the strain	1191:1220	The cellulases from the strain hydrolyzed alkali treated rice straw and bagasse into fermentable sugars efficiently.
27744242	8	49	theme	alkali	1233:1238	arg1	straw					1253:1257	alkali treated rice straw	1233:1257	alkali treated rice straw	1233:1257	The cellulases from the strain hydrolyzed alkali treated rice straw and bagasse into fermentable sugars efficiently.
27744242	4	50	theme	endoglucanases	481:494	arg1	profile					470:476	The sequential and differential expression profile	427:476	The sequential and differential expression profile of endoglucanases, β-glucosidases, cellobiohydrolases and xylanases using zymography	427:561	The sequential and differential expression profile of endoglucanases, β-glucosidases, cellobiohydrolases and xylanases using zymography was studied.
27744242	1	51	theme	Scytalidium	31:41	arg1	array					82:86	a diverse array	72:86	a diverse array of efficient cellulases and hemicellulases	72:129	Scytalidium thermophilum): Repertoire of a diverse array of efficient cellulases and hemicellulases in the secretome revealed.
27744242	1	51	theme	Scytalidium	31:41	arg1	Repertoire					58:67	Scytalidium thermophilum): Repertoire	31:67	Scytalidium thermophilum): Repertoire of a diverse array of efficient cellulases and hemicellulases in the secretome	31:146	Scytalidium thermophilum): Repertoire of a diverse array of efficient cellulases and hemicellulases in the secretome revealed.
27744242	5	52	theme	spectrometry	581:592	arg1	analysis					594:601	Mass spectrometry analysis	576:601	Mass spectrometry analysis of secretome (Q-TOF LC/MS)	576:628	Mass spectrometry analysis of secretome (Q-TOF LC/MS) revealed a total of 240 proteins with 92 CAZymes of which 62 glycosyl hydrolases belonging to 30 different families were present.
27744242	3	53	theme	shake	345:349	arg1	culturing					357:365	shake flask culturing	345:365	shake flask culturing on cellulose/wheat-bran/rice straw based production medium	345:424	Scytalidium thermophilum/Humicola insolens), a thermophilic fungus, is being reported to produce appreciable titers of cellulases and hemicellulases during shake flask culturing on cellulose/wheat-bran/rice straw based production medium.
27744242	6	54	from	proteins	883:890	arg1	secretome					899:907	the secretome	895:907	the secretome	895:907	Cellobiohydrolase I (17.42%), β glucosidase (8.69%), endoglucanase (6.2%), xylanase (4.16%) and AA9 (3.95%) were the major proteins in the secretome.
27744242	0	55	dep	thermophilus	12:23	arg1	Syn					26:28	Syn	26:28	Syn	26:28	Mycothermus thermophilus (Syn.
27744242	7	56	theme	polysaccharide	947:960	arg1	lyases					962:967	polysaccharide lyases	947:967	polysaccharide lyases	947:967	In addition, carbohydrate esterases, polysaccharide lyases, auxiliary activity and a variety of carbohydrate binding modules (CBM) were identified using genomic database of the culture indicating to an elaborate genetic potential of this strain for hydrolysis of lignocellulosics.
27744242	8	57	theme	fermentable	1276:1286	arg1	sugars					1288:1293	fermentable sugars	1276:1293	fermentable sugars	1276:1293	The cellulases from the strain hydrolyzed alkali treated rice straw and bagasse into fermentable sugars efficiently.
27744242	3	58	theme	flask	351:355	arg1	culturing					357:365	shake flask culturing	345:365	shake flask culturing on cellulose/wheat-bran/rice straw based production medium	345:424	Scytalidium thermophilum/Humicola insolens), a thermophilic fungus, is being reported to produce appreciable titers of cellulases and hemicellulases during shake flask culturing on cellulose/wheat-bran/rice straw based production medium.
27744242	6	59	theme	major	877:881	arg1	Cellobiohydrolase					760:776	Cellobiohydrolase I	760:778	Cellobiohydrolase I (17.42%)	760:787	Cellobiohydrolase I (17.42%), β glucosidase (8.69%), endoglucanase (6.2%), xylanase (4.16%) and AA9 (3.95%) were the major proteins in the secretome.
27744242	6	59	theme	major	877:881	arg1	xylanase					835:842	xylanase	835:842	xylanase (4.16%)	835:850	Cellobiohydrolase I (17.42%), β glucosidase (8.69%), endoglucanase (6.2%), xylanase (4.16%) and AA9 (3.95%) were the major proteins in the secretome.
27744242	6	59	theme	major	877:881	arg1	AA9					856:858	AA9	856:858	AA9 (3.95%)	856:866	Cellobiohydrolase I (17.42%), β glucosidase (8.69%), endoglucanase (6.2%), xylanase (4.16%) and AA9 (3.95%) were the major proteins in the secretome.
27744242	6	59	theme	major	877:881	arg1	glucosidase					792:802	β glucosidase	790:802	β glucosidase (8.69%)	790:810	Cellobiohydrolase I (17.42%), β glucosidase (8.69%), endoglucanase (6.2%), xylanase (4.16%) and AA9 (3.95%) were the major proteins in the secretome.
27744242	6	59	theme	major	877:881	arg1	endoglucanase					813:825	endoglucanase	813:825	endoglucanase (6.2%)	813:832	Cellobiohydrolase I (17.42%), β glucosidase (8.69%), endoglucanase (6.2%), xylanase (4.16%) and AA9 (3.95%) were the major proteins in the secretome.
27744242	6	59	theme	major	877:881	arg1	proteins					883:890	the major proteins	873:890	the major proteins in the secretome	873:907	Cellobiohydrolase I (17.42%), β glucosidase (8.69%), endoglucanase (6.2%), xylanase (4.16%) and AA9 (3.95%) were the major proteins in the secretome.
27744242	7	60	theme	carbohydrate	923:934	arg1	esterases					936:944	carbohydrate esterases	923:944	carbohydrate esterases	923:944	In addition, carbohydrate esterases, polysaccharide lyases, auxiliary activity and a variety of carbohydrate binding modules (CBM) were identified using genomic database of the culture indicating to an elaborate genetic potential of this strain for hydrolysis of lignocellulosics.
25037376	3	0	theme	water-retention	692:706	arg1	capabilities					708:719	the swelling and water-retention capabilities	675:719	capabilities	708:719	The loess content, pH values, surfactants, salts and temperature which could affect the swelling and water-retention capabilities of the composite superabsorbent were investigated.
25037376	1	1	theme	composite	157:165	arg1	superabsorbent					167:180	A new, low-cost, and eco-friendly organic-inorganic composite superabsorbent	105:180	A new, low-cost, and eco-friendly organic-inorganic composite superabsorbent	105:180	A new, low-cost, and eco-friendly organic-inorganic composite superabsorbent was successfully synthesized in aqueous solution by polymerization xanthan gum (XG), neutralized acrylic acid (AA) and loess using ammonium persulfate (APS) as initiator and N,N-methylenebisacrylamide (MBA) as crosslinker.
25037376	4	2	theme	metal	1037:1041	arg1	ions					1043:1046	multivalent metal ions	1025:1046	multivalent metal ions	1025:1046	The composite superabsorbent exhibits excellent water absorbency (610 g/g in distilled water), pH-stability (pH 5-10), and higher swelling capacity in anionic surfactant solution; on the other hand, the composite superabsorbent can be used for removing multivalent metal ions.
25037376	4	3	used	used	1007:1010	arg2	superabsorbent					985:998	the composite superabsorbent	971:998	the composite superabsorbent	971:998	The composite superabsorbent exhibits excellent water absorbency (610 g/g in distilled water), pH-stability (pH 5-10), and higher swelling capacity in anionic surfactant solution; on the other hand, the composite superabsorbent can be used for removing multivalent metal ions.
25037376	3	4	theme	swelling	679:686	arg1	capabilities					708:719	the swelling and water-retention capabilities	675:719	capabilities	708:719	The loess content, pH values, surfactants, salts and temperature which could affect the swelling and water-retention capabilities of the composite superabsorbent were investigated.
25037376	4	5	theme	composite	776:784	arg1	superabsorbent					786:799	The composite superabsorbent	772:799	The composite superabsorbent	772:799	The composite superabsorbent exhibits excellent water absorbency (610 g/g in distilled water), pH-stability (pH 5-10), and higher swelling capacity in anionic surfactant solution; on the other hand, the composite superabsorbent can be used for removing multivalent metal ions.
25037376	4	6	theme	composite	975:983	arg1	superabsorbent					985:998	the composite superabsorbent	971:998	the composite superabsorbent	971:998	The composite superabsorbent exhibits excellent water absorbency (610 g/g in distilled water), pH-stability (pH 5-10), and higher swelling capacity in anionic surfactant solution; on the other hand, the composite superabsorbent can be used for removing multivalent metal ions.
25037376	1	7	theme	acrylic	279:285	arg1	AA					293:294	AA	293:294	AA	293:294	A new, low-cost, and eco-friendly organic-inorganic composite superabsorbent was successfully synthesized in aqueous solution by polymerization xanthan gum (XG), neutralized acrylic acid (AA) and loess using ammonium persulfate (APS) as initiator and N,N-methylenebisacrylamide (MBA) as crosslinker.
25037376	1	7	theme	acrylic	279:285	arg1	acid					287:290	acrylic acid	279:290	acrylic acid (AA)	279:295	A new, low-cost, and eco-friendly organic-inorganic composite superabsorbent was successfully synthesized in aqueous solution by polymerization xanthan gum (XG), neutralized acrylic acid (AA) and loess using ammonium persulfate (APS) as initiator and N,N-methylenebisacrylamide (MBA) as crosslinker.
25037376	3	8	theme	composite	728:736	arg1	superabsorbent					738:751	the composite superabsorbent	724:751	the composite superabsorbent	724:751	The loess content, pH values, surfactants, salts and temperature which could affect the swelling and water-retention capabilities of the composite superabsorbent were investigated.
25037376	2	9	theme	infrared	522:529	arg1	spectroscopy					531:542	Fourier-transform infrared spectroscopy	504:542	Fourier-transform infrared spectroscopy (FTIR)	504:549	Structure and morphological characterizations of the composite superabsorbent were investigated by Fourier-transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM).
25037376	2	9	theme	infrared	522:529	arg1	FTIR					545:548	FTIR	545:548	FTIR	545:548	Structure and morphological characterizations of the composite superabsorbent were investigated by Fourier-transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM).
25037376	0	10	theme	composite	48:56	arg1	superabsorbent					58:71	organic-inorganic composite superabsorbent	30:71	organic-inorganic composite superabsorbent	30:71	Preparation and properties of organic-inorganic composite superabsorbent based on xanthan gum and loess.
25037376	4	11	dep	absorbency	826:835	arg1	g/g					842:844	610 g/g	838:844	610 g/g in distilled water	838:863	The composite superabsorbent exhibits excellent water absorbency (610 g/g in distilled water), pH-stability (pH 5-10), and higher swelling capacity in anionic surfactant solution; on the other hand, the composite superabsorbent can be used for removing multivalent metal ions.
25037376	0	12	theme	xanthan	82:88	arg1	gum					90:92	xanthan gum	82:92	xanthan gum	82:92	Preparation and properties of organic-inorganic composite superabsorbent based on xanthan gum and loess.
25037376	2	13	theme	morphological	419:431	arg1	characterizations					433:449	morphological characterizations	419:449	morphological characterizations	419:449	Structure and morphological characterizations of the composite superabsorbent were investigated by Fourier-transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM).
25037376	4	14	theme	multivalent	1025:1035	arg1	ions					1043:1046	multivalent metal ions	1025:1046	multivalent metal ions	1025:1046	The composite superabsorbent exhibits excellent water absorbency (610 g/g in distilled water), pH-stability (pH 5-10), and higher swelling capacity in anionic surfactant solution; on the other hand, the composite superabsorbent can be used for removing multivalent metal ions.
25037376	2	15	theme	electron	564:571	arg1	SEM					585:587	SEM	585:587	SEM	585:587	Structure and morphological characterizations of the composite superabsorbent were investigated by Fourier-transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM).
25037376	2	15	theme	electron	564:571	arg1	microscopy					573:582	scanning electron microscopy	555:582	scanning electron microscopy (SEM)	555:588	Structure and morphological characterizations of the composite superabsorbent were investigated by Fourier-transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM).
25037376	1	16	theme	aqueous	214:220	arg1	solution					222:229	aqueous solution	214:229	aqueous solution	214:229	A new, low-cost, and eco-friendly organic-inorganic composite superabsorbent was successfully synthesized in aqueous solution by polymerization xanthan gum (XG), neutralized acrylic acid (AA) and loess using ammonium persulfate (APS) as initiator and N,N-methylenebisacrylamide (MBA) as crosslinker.
25037376	3	17	theme	superabsorbent	738:751	arg1	capabilities					708:719	the swelling and water-retention capabilities	675:719	capabilities	708:719	The loess content, pH values, surfactants, salts and temperature which could affect the swelling and water-retention capabilities of the composite superabsorbent were investigated.
25037376	2	18	theme	scanning	555:562	arg1	SEM					585:587	SEM	585:587	SEM	585:587	Structure and morphological characterizations of the composite superabsorbent were investigated by Fourier-transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM).
25037376	2	18	theme	scanning	555:562	arg1	microscopy					573:582	scanning electron microscopy	555:582	scanning electron microscopy (SEM)	555:588	Structure and morphological characterizations of the composite superabsorbent were investigated by Fourier-transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM).
25037376	4	19	theme	anionic	923:929	arg1	solution					942:949	anionic surfactant solution	923:949	anionic surfactant solution	923:949	The composite superabsorbent exhibits excellent water absorbency (610 g/g in distilled water), pH-stability (pH 5-10), and higher swelling capacity in anionic surfactant solution; on the other hand, the composite superabsorbent can be used for removing multivalent metal ions.
25037376	1	20	theme	new	107:109	arg1	superabsorbent					167:180	A new, low-cost, and eco-friendly organic-inorganic composite superabsorbent	105:180	A new, low-cost, and eco-friendly organic-inorganic composite superabsorbent	105:180	A new, low-cost, and eco-friendly organic-inorganic composite superabsorbent was successfully synthesized in aqueous solution by polymerization xanthan gum (XG), neutralized acrylic acid (AA) and loess using ammonium persulfate (APS) as initiator and N,N-methylenebisacrylamide (MBA) as crosslinker.
25037376	2	21	theme	superabsorbent	468:481	arg1	Structure					405:413	Structure	405:413	Structure	405:413	Structure and morphological characterizations of the composite superabsorbent were investigated by Fourier-transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM).
25037376	2	21	theme	superabsorbent	468:481	arg1	characterizations					433:449	morphological characterizations	419:449	morphological characterizations	419:449	Structure and morphological characterizations of the composite superabsorbent were investigated by Fourier-transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM).
25037376	4	22	theme	surfactant	931:940	arg1	solution					942:949	anionic surfactant solution	923:949	anionic surfactant solution	923:949	The composite superabsorbent exhibits excellent water absorbency (610 g/g in distilled water), pH-stability (pH 5-10), and higher swelling capacity in anionic surfactant solution; on the other hand, the composite superabsorbent can be used for removing multivalent metal ions.
25037376	3	23	theme	loess	595:599	arg1	content					601:607	The loess content	591:607	The loess content	591:607	The loess content, pH values, surfactants, salts and temperature which could affect the swelling and water-retention capabilities of the composite superabsorbent were investigated.
25037376	0	24	theme	organic-inorganic	30:46	arg1	superabsorbent					58:71	organic-inorganic composite superabsorbent	30:71	organic-inorganic composite superabsorbent	30:71	Preparation and properties of organic-inorganic composite superabsorbent based on xanthan gum and loess.
25037376	2	25	theme	composite	458:466	arg1	superabsorbent					468:481	the composite superabsorbent	454:481	the composite superabsorbent	454:481	Structure and morphological characterizations of the composite superabsorbent were investigated by Fourier-transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM).
25037376	1	26	theme	polymerization	234:247	arg1	XG					262:263	XG	262:263	XG	262:263	A new, low-cost, and eco-friendly organic-inorganic composite superabsorbent was successfully synthesized in aqueous solution by polymerization xanthan gum (XG), neutralized acrylic acid (AA) and loess using ammonium persulfate (APS) as initiator and N,N-methylenebisacrylamide (MBA) as crosslinker.
25037376	1	26	theme	polymerization	234:247	arg1	gum					257:259	polymerization xanthan gum	234:259	polymerization xanthan gum (XG)	234:264	A new, low-cost, and eco-friendly organic-inorganic composite superabsorbent was successfully synthesized in aqueous solution by polymerization xanthan gum (XG), neutralized acrylic acid (AA) and loess using ammonium persulfate (APS) as initiator and N,N-methylenebisacrylamide (MBA) as crosslinker.
25037376	4	27	theme	distilled	849:857	arg1	water					859:863	distilled water	849:863	distilled water	849:863	The composite superabsorbent exhibits excellent water absorbency (610 g/g in distilled water), pH-stability (pH 5-10), and higher swelling capacity in anionic surfactant solution; on the other hand, the composite superabsorbent can be used for removing multivalent metal ions.
25037376	0	28	theme	superabsorbent	58:71	arg1	properties					16:25	properties	16:25	properties	16:25	Preparation and properties of organic-inorganic composite superabsorbent based on xanthan gum and loess.
25037376	0	28	theme	superabsorbent	58:71	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and properties of organic-inorganic composite superabsorbent based on xanthan gum and loess.
25037376	1	29	dep	neutralized	267:277	arg1	using					307:311	using	307:311	using ammonium persulfate (APS) as initiator and N,N-methylenebisacrylamide (MBA) as crosslinker	307:402	A new, low-cost, and eco-friendly organic-inorganic composite superabsorbent was successfully synthesized in aqueous solution by polymerization xanthan gum (XG), neutralized acrylic acid (AA) and loess using ammonium persulfate (APS) as initiator and N,N-methylenebisacrylamide (MBA) as crosslinker.
25037376	4	30	from	g/g	842:844	arg1	water					859:863	distilled water	849:863	distilled water	849:863	The composite superabsorbent exhibits excellent water absorbency (610 g/g in distilled water), pH-stability (pH 5-10), and higher swelling capacity in anionic surfactant solution; on the other hand, the composite superabsorbent can be used for removing multivalent metal ions.
25037376	1	31	theme	low-cost	112:119	arg1	superabsorbent					167:180	A new, low-cost, and eco-friendly organic-inorganic composite superabsorbent	105:180	A new, low-cost, and eco-friendly organic-inorganic composite superabsorbent	105:180	A new, low-cost, and eco-friendly organic-inorganic composite superabsorbent was successfully synthesized in aqueous solution by polymerization xanthan gum (XG), neutralized acrylic acid (AA) and loess using ammonium persulfate (APS) as initiator and N,N-methylenebisacrylamide (MBA) as crosslinker.
25037376	1	32	theme	ammonium	313:320	arg1	APS					334:336	APS	334:336	APS	334:336	A new, low-cost, and eco-friendly organic-inorganic composite superabsorbent was successfully synthesized in aqueous solution by polymerization xanthan gum (XG), neutralized acrylic acid (AA) and loess using ammonium persulfate (APS) as initiator and N,N-methylenebisacrylamide (MBA) as crosslinker.
25037376	1	32	theme	ammonium	313:320	arg1	persulfate					322:331	ammonium persulfate	313:331	ammonium persulfate (APS)	313:337	A new, low-cost, and eco-friendly organic-inorganic composite superabsorbent was successfully synthesized in aqueous solution by polymerization xanthan gum (XG), neutralized acrylic acid (AA) and loess using ammonium persulfate (APS) as initiator and N,N-methylenebisacrylamide (MBA) as crosslinker.
25037376	4	33	theme	swelling	902:909	arg1	capacity					911:918	higher swelling capacity	895:918	higher swelling capacity	895:918	The composite superabsorbent exhibits excellent water absorbency (610 g/g in distilled water), pH-stability (pH 5-10), and higher swelling capacity in anionic surfactant solution; on the other hand, the composite superabsorbent can be used for removing multivalent metal ions.
25037376	4	34	theme	water	820:824	arg1	absorbency					826:835	excellent water absorbency	810:835	excellent water absorbency (610 g/g in distilled water)	810:864	The composite superabsorbent exhibits excellent water absorbency (610 g/g in distilled water), pH-stability (pH 5-10), and higher swelling capacity in anionic surfactant solution; on the other hand, the composite superabsorbent can be used for removing multivalent metal ions.
25037376	1	35	theme	xanthan	249:255	arg1	XG					262:263	XG	262:263	XG	262:263	A new, low-cost, and eco-friendly organic-inorganic composite superabsorbent was successfully synthesized in aqueous solution by polymerization xanthan gum (XG), neutralized acrylic acid (AA) and loess using ammonium persulfate (APS) as initiator and N,N-methylenebisacrylamide (MBA) as crosslinker.
25037376	1	35	theme	xanthan	249:255	arg1	gum					257:259	polymerization xanthan gum	234:259	polymerization xanthan gum (XG)	234:264	A new, low-cost, and eco-friendly organic-inorganic composite superabsorbent was successfully synthesized in aqueous solution by polymerization xanthan gum (XG), neutralized acrylic acid (AA) and loess using ammonium persulfate (APS) as initiator and N,N-methylenebisacrylamide (MBA) as crosslinker.
25037376	4	36	theme	higher	895:900	arg1	capacity					911:918	higher swelling capacity	895:918	higher swelling capacity	895:918	The composite superabsorbent exhibits excellent water absorbency (610 g/g in distilled water), pH-stability (pH 5-10), and higher swelling capacity in anionic surfactant solution; on the other hand, the composite superabsorbent can be used for removing multivalent metal ions.
25037376	4	37	theme	excellent	810:818	arg1	absorbency					826:835	excellent water absorbency	810:835	excellent water absorbency (610 g/g in distilled water)	810:864	The composite superabsorbent exhibits excellent water absorbency (610 g/g in distilled water), pH-stability (pH 5-10), and higher swelling capacity in anionic surfactant solution; on the other hand, the composite superabsorbent can be used for removing multivalent metal ions.
25037376	2	38	theme	Fourier-transform	504:520	arg1	spectroscopy					531:542	Fourier-transform infrared spectroscopy	504:542	Fourier-transform infrared spectroscopy (FTIR)	504:549	Structure and morphological characterizations of the composite superabsorbent were investigated by Fourier-transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM).
25037376	2	38	theme	Fourier-transform	504:520	arg1	FTIR					545:548	FTIR	545:548	FTIR	545:548	Structure and morphological characterizations of the composite superabsorbent were investigated by Fourier-transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM).
25037376	4	39	theme	other	959:963	arg1	hand					965:968	the other hand	955:968	the other hand	955:968	The composite superabsorbent exhibits excellent water absorbency (610 g/g in distilled water), pH-stability (pH 5-10), and higher swelling capacity in anionic surfactant solution; on the other hand, the composite superabsorbent can be used for removing multivalent metal ions.
25037376	3	40	theme	pH	610:611	arg1	values					613:618	pH values	610:618	pH values	610:618	The loess content, pH values, surfactants, salts and temperature which could affect the swelling and water-retention capabilities of the composite superabsorbent were investigated.
25037376	1	41	theme	organic-inorganic	139:155	arg1	superabsorbent					167:180	A new, low-cost, and eco-friendly organic-inorganic composite superabsorbent	105:180	A new, low-cost, and eco-friendly organic-inorganic composite superabsorbent	105:180	A new, low-cost, and eco-friendly organic-inorganic composite superabsorbent was successfully synthesized in aqueous solution by polymerization xanthan gum (XG), neutralized acrylic acid (AA) and loess using ammonium persulfate (APS) as initiator and N,N-methylenebisacrylamide (MBA) as crosslinker.
25036733	5	0	dep	[formula	839:846	arg1	see					849:851	see	849:851	see text	849:856	The structure of the serotype 39 capsular polysaccharide is [formula: see text] and has identical composition to the capsular polysaccharide 10A, but two different linkages.
25036733	3	1	theme	capsular	528:535	arg1	polysaccharides					537:551	the capsular polysaccharides	524:551	the capsular polysaccharides of serotypes 39, 42, and 47F from the genetic cluster 4	524:607	Here, we report the chemical structures of the capsular polysaccharides of serotypes 39, 42, and 47F from the genetic cluster 4, and discuss the structures in the context of structures from serologically and genetically related serotypes.
25036733	7	2	theme	47F	1413:1415	arg1	cross-reactivity					1384:1399	the cross-reactivity	1380:1399	the cross-reactivity of serotype 47F with the typing serum 35a	1380:1441	The structure of the serotype 47F capsular polysaccharide [formula: see text] is somewhat different from a recently determined structure from the same serogroup, while containing a structural motif that is reflected in serotype 35A and 42 capsular polysaccharide structures, thus explaining the cross-reactivity of serotype 47F with the typing serum 35a.
25036733	6	3	contain	contain	1070:1076	arg2	A					1086:1086	residue A	1078:1086	residue A	1078:1086	The serotype 42 structure [formula: see text] closely resembles the genetically related serotype 35A, which does not contain residue A.
25036733	6	3	contain	contain	1070:1076	arg1	35A					1050:1052	the genetically related serotype 35A	1017:1052	the genetically related serotype 35A	1017:1052	The serotype 42 structure [formula: see text] closely resembles the genetically related serotype 35A, which does not contain residue A.
25036733	7	4	theme	47F	1119:1121	arg1	polysaccharide					1132:1145	the serotype 47F capsular polysaccharide	1106:1145	the serotype 47F capsular polysaccharide [formula: see text]	1106:1165	The structure of the serotype 47F capsular polysaccharide [formula: see text] is somewhat different from a recently determined structure from the same serogroup, while containing a structural motif that is reflected in serotype 35A and 42 capsular polysaccharide structures, thus explaining the cross-reactivity of serotype 47F with the typing serum 35a.
25036733	3	5	theme	polysaccharides	537:551	arg1	structures					510:519	the chemical structures	497:519	the chemical structures of the capsular polysaccharides of serotypes 39, 42, and 47F from the genetic cluster 4	497:607	Here, we report the chemical structures of the capsular polysaccharides of serotypes 39, 42, and 47F from the genetic cluster 4, and discuss the structures in the context of structures from serologically and genetically related serotypes.
25036733	7	6	theme	serotype	1110:1117	arg1	polysaccharide					1132:1145	the serotype 47F capsular polysaccharide	1106:1145	the serotype 47F capsular polysaccharide [formula: see text]	1106:1165	The structure of the serotype 47F capsular polysaccharide [formula: see text] is somewhat different from a recently determined structure from the same serogroup, while containing a structural motif that is reflected in serotype 35A and 42 capsular polysaccharide structures, thus explaining the cross-reactivity of serotype 47F with the typing serum 35a.
25036733	3	7	theme	related	701:707	arg1	serotypes					709:717	serologically and genetically related serotypes	671:717	serologically and genetically related serotypes	671:717	Here, we report the chemical structures of the capsular polysaccharides of serotypes 39, 42, and 47F from the genetic cluster 4, and discuss the structures in the context of structures from serologically and genetically related serotypes.
25036733	3	8	from	polysaccharides	537:551	arg1	cluster					599:605	the genetic cluster 4	587:607	the genetic cluster 4	587:607	Here, we report the chemical structures of the capsular polysaccharides of serotypes 39, 42, and 47F from the genetic cluster 4, and discuss the structures in the context of structures from serologically and genetically related serotypes.
25036733	3	9	theme	42	570:571	arg1	polysaccharides					537:551	the capsular polysaccharides	524:551	the capsular polysaccharides of serotypes 39, 42, and 47F from the genetic cluster 4	524:607	Here, we report the chemical structures of the capsular polysaccharides of serotypes 39, 42, and 47F from the genetic cluster 4, and discuss the structures in the context of structures from serologically and genetically related serotypes.
25036733	3	10	dep	serotypes	556:564	arg1	39					566:567	39	566:567	39	566:567	Here, we report the chemical structures of the capsular polysaccharides of serotypes 39, 42, and 47F from the genetic cluster 4, and discuss the structures in the context of structures from serologically and genetically related serotypes.
25036733	7	11	theme	serotype	1404:1411	arg1	47F					1413:1415	serotype 47F	1404:1415	serotype 47F	1404:1415	The structure of the serotype 47F capsular polysaccharide [formula: see text] is somewhat different from a recently determined structure from the same serogroup, while containing a structural motif that is reflected in serotype 35A and 42 capsular polysaccharide structures, thus explaining the cross-reactivity of serotype 47F with the typing serum 35a.
25036733	7	12	dep	see	1157:1159	arg1	[formula					1147:1154	[formula	1147:1154	[formula	1147:1154	The structure of the serotype 47F capsular polysaccharide [formula: see text] is somewhat different from a recently determined structure from the same serogroup, while containing a structural motif that is reflected in serotype 35A and 42 capsular polysaccharide structures, thus explaining the cross-reactivity of serotype 47F with the typing serum 35a.
25036733	7	13	theme	same	1235:1238	arg1	serogroup					1240:1248	the same serogroup	1231:1248	the same serogroup	1231:1248	The structure of the serotype 47F capsular polysaccharide [formula: see text] is somewhat different from a recently determined structure from the same serogroup, while containing a structural motif that is reflected in serotype 35A and 42 capsular polysaccharide structures, thus explaining the cross-reactivity of serotype 47F with the typing serum 35a.
25036733	7	14	theme	serum	1433:1437	arg1	35a					1439:1441	the typing serum 35a	1422:1441	the typing serum 35a	1422:1441	The structure of the serotype 47F capsular polysaccharide [formula: see text] is somewhat different from a recently determined structure from the same serogroup, while containing a structural motif that is reflected in serotype 35A and 42 capsular polysaccharide structures, thus explaining the cross-reactivity of serotype 47F with the typing serum 35a.
25036733	5	15	theme	different	933:941	arg1	linkages					943:950	two different linkages	929:950	two different linkages	929:950	The structure of the serotype 39 capsular polysaccharide is [formula: see text] and has identical composition to the capsular polysaccharide 10A, but two different linkages.
25036733	3	16	from	context	644:650	arg1	serotypes					709:717	serologically and genetically related serotypes	671:717	serologically and genetically related serotypes	671:717	Here, we report the chemical structures of the capsular polysaccharides of serotypes 39, 42, and 47F from the genetic cluster 4, and discuss the structures in the context of structures from serologically and genetically related serotypes.
25036733	2	17	theme	polysaccharide	435:448	arg1	structures					450:459	resultant polysaccharide structures	425:459	resultant polysaccharide structures	425:459	While the genetic loci for capsular biosynthesis have been characterized for all serotypes, the determination of resultant polysaccharide structures remains incomplete.
25036733	6	18	theme	related	1033:1039	arg1	35A					1050:1052	the genetically related serotype 35A	1017:1052	the genetically related serotype 35A	1017:1052	The serotype 42 structure [formula: see text] closely resembles the genetically related serotype 35A, which does not contain residue A.
25036733	6	19	theme	residue	1078:1084	arg1	A					1086:1086	residue A	1078:1086	residue A	1078:1086	The serotype 42 structure [formula: see text] closely resembles the genetically related serotype 35A, which does not contain residue A.
25036733	7	20	theme	capsular	1123:1130	arg1	polysaccharide					1132:1145	the serotype 47F capsular polysaccharide	1106:1145	the serotype 47F capsular polysaccharide [formula: see text]	1106:1165	The structure of the serotype 47F capsular polysaccharide [formula: see text] is somewhat different from a recently determined structure from the same serogroup, while containing a structural motif that is reflected in serotype 35A and 42 capsular polysaccharide structures, thus explaining the cross-reactivity of serotype 47F with the typing serum 35a.
25036733	7	21	dep	polysaccharide	1132:1145	arg1	see					1157:1159	see	1157:1159	see text	1157:1164	The structure of the serotype 47F capsular polysaccharide [formula: see text] is somewhat different from a recently determined structure from the same serogroup, while containing a structural motif that is reflected in serotype 35A and 42 capsular polysaccharide structures, thus explaining the cross-reactivity of serotype 47F with the typing serum 35a.
25036733	5	22	theme	identical	867:875	arg1	composition					877:887	identical composition	867:887	identical composition to the capsular polysaccharide 10A, but two different linkages	867:950	The structure of the serotype 39 capsular polysaccharide is [formula: see text] and has identical composition to the capsular polysaccharide 10A, but two different linkages.
25036733	5	23	theme	polysaccharide	821:834	arg1	[formula					839:846	[formula	839:846	[formula: see text]	839:857	The structure of the serotype 39 capsular polysaccharide is [formula: see text] and has identical composition to the capsular polysaccharide 10A, but two different linkages.
25036733	5	23	theme	polysaccharide	821:834	arg1	structure					783:791	The structure	779:791	The structure of the serotype 39 capsular polysaccharide	779:834	The structure of the serotype 39 capsular polysaccharide is [formula: see text] and has identical composition to the capsular polysaccharide 10A, but two different linkages.
25036733	7	24	theme	determined	1205:1214	arg1	structure					1216:1224	a recently determined structure	1194:1224	a recently determined structure from the same serogroup	1194:1248	The structure of the serotype 47F capsular polysaccharide [formula: see text] is somewhat different from a recently determined structure from the same serogroup, while containing a structural motif that is reflected in serotype 35A and 42 capsular polysaccharide structures, thus explaining the cross-reactivity of serotype 47F with the typing serum 35a.
25036733	5	25	dep	polysaccharide	905:918	arg1	10A					920:922	10A	920:922	10A	920:922	The structure of the serotype 39 capsular polysaccharide is [formula: see text] and has identical composition to the capsular polysaccharide 10A, but two different linkages.
25036733	3	26	from	structures	626:635	arg1	context					644:650	the context	640:650	the context of structures from serologically and genetically related serotypes	640:717	Here, we report the chemical structures of the capsular polysaccharides of serotypes 39, 42, and 47F from the genetic cluster 4, and discuss the structures in the context of structures from serologically and genetically related serotypes.
25036733	7	27	from	structure	1216:1224	arg1	structure					1093:1101	The structure	1089:1101	The structure of the serotype 47F capsular polysaccharide [formula: see text]	1089:1165	The structure of the serotype 47F capsular polysaccharide [formula: see text] is somewhat different from a recently determined structure from the same serogroup, while containing a structural motif that is reflected in serotype 35A and 42 capsular polysaccharide structures, thus explaining the cross-reactivity of serotype 47F with the typing serum 35a.
25036733	7	27	from	structure	1216:1224	arg1	different					1179:1187	different	1179:1187	different	1179:1187	The structure of the serotype 47F capsular polysaccharide [formula: see text] is somewhat different from a recently determined structure from the same serogroup, while containing a structural motif that is reflected in serotype 35A and 42 capsular polysaccharide structures, thus explaining the cross-reactivity of serotype 47F with the typing serum 35a.
25036733	7	28	with	cross-reactivity	1384:1399	arg1	35a					1439:1441	the typing serum 35a	1422:1441	the typing serum 35a	1422:1441	The structure of the serotype 47F capsular polysaccharide [formula: see text] is somewhat different from a recently determined structure from the same serogroup, while containing a structural motif that is reflected in serotype 35A and 42 capsular polysaccharide structures, thus explaining the cross-reactivity of serotype 47F with the typing serum 35a.
25036733	2	29	theme	genetic	322:328	arg1	loci					330:333	the genetic loci	318:333	the genetic loci for capsular biosynthesis	318:359	While the genetic loci for capsular biosynthesis have been characterized for all serotypes, the determination of resultant polysaccharide structures remains incomplete.
25036733	7	30	theme	serotype	1308:1315	arg1	35A					1317:1319	serotype 35A	1308:1319	serotype 35A	1308:1319	The structure of the serotype 47F capsular polysaccharide [formula: see text] is somewhat different from a recently determined structure from the same serogroup, while containing a structural motif that is reflected in serotype 35A and 42 capsular polysaccharide structures, thus explaining the cross-reactivity of serotype 47F with the typing serum 35a.
25036733	0	31	theme	capsular	24:31	arg1	structures					48:57	native capsular polysaccharide structures	17:57	native capsular polysaccharide structures of Streptococcus pneumoniae serotypes 39, 42, and 47F	17:111	Determination of native capsular polysaccharide structures of Streptococcus pneumoniae serotypes 39, 42, and 47F and comparison to genetically or serologically related strains.
25036733	1	32	theme	different	291:299	arg1	serotypes					301:309	at least 91 different serotypes	279:309	at least 91 different serotypes	279:309	The diversity of capsular polysaccharides of the bacterial pathogen Streptococcus pneumoniae leads to at least 91 different serotypes.
25036733	5	33	contain	has	863:865	arg1	[formula					839:846	[formula	839:846	[formula: see text]	839:857	The structure of the serotype 39 capsular polysaccharide is [formula: see text] and has identical composition to the capsular polysaccharide 10A, but two different linkages.
25036733	5	33	contain	has	863:865	arg2	composition					877:887	identical composition	867:887	identical composition to the capsular polysaccharide 10A, but two different linkages	867:950	The structure of the serotype 39 capsular polysaccharide is [formula: see text] and has identical composition to the capsular polysaccharide 10A, but two different linkages.
25036733	5	33	contain	has	863:865	arg1	structure					783:791	The structure	779:791	The structure of the serotype 39 capsular polysaccharide	779:834	The structure of the serotype 39 capsular polysaccharide is [formula: see text] and has identical composition to the capsular polysaccharide 10A, but two different linkages.
25036733	3	34	from	cluster	599:605	arg1	polysaccharides					537:551	the capsular polysaccharides	524:551	the capsular polysaccharides of serotypes 39, 42, and 47F from the genetic cluster 4	524:607	Here, we report the chemical structures of the capsular polysaccharides of serotypes 39, 42, and 47F from the genetic cluster 4, and discuss the structures in the context of structures from serologically and genetically related serotypes.
25036733	3	34	from	cluster	599:605	arg1	47F					578:580	47F	578:580	47F	578:580	Here, we report the chemical structures of the capsular polysaccharides of serotypes 39, 42, and 47F from the genetic cluster 4, and discuss the structures in the context of structures from serologically and genetically related serotypes.
25036733	3	34	from	cluster	599:605	arg1	serotypes					556:564	serotypes 39, 42, and 47F	556:580	serotypes	556:564	Here, we report the chemical structures of the capsular polysaccharides of serotypes 39, 42, and 47F from the genetic cluster 4, and discuss the structures in the context of structures from serologically and genetically related serotypes.
25036733	2	35	theme	structures	450:459	arg1	determination					408:420	the determination	404:420	the determination of resultant polysaccharide structures	404:459	While the genetic loci for capsular biosynthesis have been characterized for all serotypes, the determination of resultant polysaccharide structures remains incomplete.
25036733	0	36	theme	native	17:22	arg1	structures					48:57	native capsular polysaccharide structures	17:57	native capsular polysaccharide structures of Streptococcus pneumoniae serotypes 39, 42, and 47F	17:111	Determination of native capsular polysaccharide structures of Streptococcus pneumoniae serotypes 39, 42, and 47F and comparison to genetically or serologically related strains.
25036733	6	37	theme	structure	969:977	arg1	[formula					979:986	The serotype 42 structure [formula	953:986	The serotype 42 structure [formula: see text]	953:997	The serotype 42 structure [formula: see text] closely resembles the genetically related serotype 35A, which does not contain residue A.
25036733	3	38	theme	47F	578:580	arg1	polysaccharides					537:551	the capsular polysaccharides	524:551	the capsular polysaccharides of serotypes 39, 42, and 47F from the genetic cluster 4	524:607	Here, we report the chemical structures of the capsular polysaccharides of serotypes 39, 42, and 47F from the genetic cluster 4, and discuss the structures in the context of structures from serologically and genetically related serotypes.
25036733	1	39	theme	polysaccharides	203:217	arg1	diversity					181:189	The diversity	177:189	The diversity of capsular polysaccharides of the bacterial pathogen Streptococcus pneumoniae	177:268	The diversity of capsular polysaccharides of the bacterial pathogen Streptococcus pneumoniae leads to at least 91 different serotypes.
25036733	0	40	theme	structures	48:57	arg1	Determination					0:12	Determination	0:12	Determination of native capsular polysaccharide structures of Streptococcus pneumoniae serotypes 39, 42, and 47F	0:111	Determination of native capsular polysaccharide structures of Streptococcus pneumoniae serotypes 39, 42, and 47F and comparison to genetically or serologically related strains.
25036733	0	40	theme	structures	48:57	arg1	comparison					117:126	comparison	117:126	comparison to genetically or serologically related strains	117:174	Determination of native capsular polysaccharide structures of Streptococcus pneumoniae serotypes 39, 42, and 47F and comparison to genetically or serologically related strains.
25036733	7	41	theme	structural	1270:1279	arg1	motif					1281:1285	a structural motif	1268:1285	a structural motif that is reflected in serotype 35A and 42 capsular polysaccharide structures, thus explaining the cross-reactivity of serotype 47F with the typing serum 35a	1268:1441	The structure of the serotype 47F capsular polysaccharide [formula: see text] is somewhat different from a recently determined structure from the same serogroup, while containing a structural motif that is reflected in serotype 35A and 42 capsular polysaccharide structures, thus explaining the cross-reactivity of serotype 47F with the typing serum 35a.
25036733	0	42	dep	Streptococcus	62:74	arg1	pneumoniae					76:85	pneumoniae	76:85	pneumoniae	76:85	Determination of native capsular polysaccharide structures of Streptococcus pneumoniae serotypes 39, 42, and 47F and comparison to genetically or serologically related strains.
25036733	2	43	theme	resultant	425:433	arg1	structures					450:459	resultant polysaccharide structures	425:459	resultant polysaccharide structures	425:459	While the genetic loci for capsular biosynthesis have been characterized for all serotypes, the determination of resultant polysaccharide structures remains incomplete.
25036733	0	44	theme	polysaccharide	33:46	arg1	structures					48:57	native capsular polysaccharide structures	17:57	native capsular polysaccharide structures of Streptococcus pneumoniae serotypes 39, 42, and 47F	17:111	Determination of native capsular polysaccharide structures of Streptococcus pneumoniae serotypes 39, 42, and 47F and comparison to genetically or serologically related strains.
25036733	6	45	theme	serotype	957:964	arg1	[formula					979:986	The serotype 42 structure [formula	953:986	The serotype 42 structure [formula: see text]	953:997	The serotype 42 structure [formula: see text] closely resembles the genetically related serotype 35A, which does not contain residue A.
25036733	5	46	theme	serotype	800:807	arg1	polysaccharide					821:834	the serotype 39 capsular polysaccharide	796:834	the serotype 39 capsular polysaccharide	796:834	The structure of the serotype 39 capsular polysaccharide is [formula: see text] and has identical composition to the capsular polysaccharide 10A, but two different linkages.
25036733	6	47	theme	serotype	1041:1048	arg1	35A					1050:1052	the genetically related serotype 35A	1017:1052	the genetically related serotype 35A	1017:1052	The serotype 42 structure [formula: see text] closely resembles the genetically related serotype 35A, which does not contain residue A.
25036733	0	48	theme	Streptococcus	62:74	arg1	serotypes					87:95	Streptococcus pneumoniae serotypes 39, 42, and 47F	62:111	Streptococcus pneumoniae serotypes 39, 42, and 47F	62:111	Determination of native capsular polysaccharide structures of Streptococcus pneumoniae serotypes 39, 42, and 47F and comparison to genetically or serologically related strains.
25036733	3	49	theme	genetic	591:597	arg1	cluster					599:605	the genetic cluster 4	587:607	the genetic cluster 4	587:607	Here, we report the chemical structures of the capsular polysaccharides of serotypes 39, 42, and 47F from the genetic cluster 4, and discuss the structures in the context of structures from serologically and genetically related serotypes.
25036733	5	50	theme	capsular	812:819	arg1	polysaccharide					821:834	the serotype 39 capsular polysaccharide	796:834	the serotype 39 capsular polysaccharide	796:834	The structure of the serotype 39 capsular polysaccharide is [formula: see text] and has identical composition to the capsular polysaccharide 10A, but two different linkages.
25036733	3	51	theme	chemical	501:508	arg1	structures					510:519	the chemical structures	497:519	the chemical structures of the capsular polysaccharides of serotypes 39, 42, and 47F from the genetic cluster 4	497:607	Here, we report the chemical structures of the capsular polysaccharides of serotypes 39, 42, and 47F from the genetic cluster 4, and discuss the structures in the context of structures from serologically and genetically related serotypes.
25036733	7	52	from	serogroup	1240:1248	arg1	structure					1216:1224	a recently determined structure	1194:1224	a recently determined structure from the same serogroup	1194:1248	The structure of the serotype 47F capsular polysaccharide [formula: see text] is somewhat different from a recently determined structure from the same serogroup, while containing a structural motif that is reflected in serotype 35A and 42 capsular polysaccharide structures, thus explaining the cross-reactivity of serotype 47F with the typing serum 35a.
25036733	4	53	theme	Antigenic	720:728	arg1	determinants					730:741	Antigenic determinants	720:741	Antigenic determinants	720:741	Antigenic determinants can be approximated in this manner.
25036733	7	54	theme	polysaccharide	1132:1145	arg1	structure					1093:1101	The structure	1089:1101	The structure of the serotype 47F capsular polysaccharide [formula: see text]	1089:1165	The structure of the serotype 47F capsular polysaccharide [formula: see text] is somewhat different from a recently determined structure from the same serogroup, while containing a structural motif that is reflected in serotype 35A and 42 capsular polysaccharide structures, thus explaining the cross-reactivity of serotype 47F with the typing serum 35a.
25036733	7	54	theme	polysaccharide	1132:1145	arg1	different					1179:1187	different	1179:1187	different	1179:1187	The structure of the serotype 47F capsular polysaccharide [formula: see text] is somewhat different from a recently determined structure from the same serogroup, while containing a structural motif that is reflected in serotype 35A and 42 capsular polysaccharide structures, thus explaining the cross-reactivity of serotype 47F with the typing serum 35a.
25036733	7	55	theme	polysaccharide	1337:1350	arg1	structures					1352:1361	42 capsular polysaccharide structures	1325:1361	42 capsular polysaccharide structures	1325:1361	The structure of the serotype 47F capsular polysaccharide [formula: see text] is somewhat different from a recently determined structure from the same serogroup, while containing a structural motif that is reflected in serotype 35A and 42 capsular polysaccharide structures, thus explaining the cross-reactivity of serotype 47F with the typing serum 35a.
25036733	2	56	theme	capsular	339:346	arg1	biosynthesis					348:359	capsular biosynthesis	339:359	capsular biosynthesis	339:359	While the genetic loci for capsular biosynthesis have been characterized for all serotypes, the determination of resultant polysaccharide structures remains incomplete.
25036733	1	57	theme	bacterial	226:234	arg1	pneumoniae					259:268	the bacterial pathogen Streptococcus pneumoniae	222:268	the bacterial pathogen Streptococcus pneumoniae	222:268	The diversity of capsular polysaccharides of the bacterial pathogen Streptococcus pneumoniae leads to at least 91 different serotypes.
25036733	0	58	theme	related	160:166	arg1	strains					168:174	genetically or serologically related strains	131:174	genetically or serologically related strains	131:174	Determination of native capsular polysaccharide structures of Streptococcus pneumoniae serotypes 39, 42, and 47F and comparison to genetically or serologically related strains.
25036733	3	59	theme	structures	655:664	arg1	context					644:650	the context	640:650	the context of structures from serologically and genetically related serotypes	640:717	Here, we report the chemical structures of the capsular polysaccharides of serotypes 39, 42, and 47F from the genetic cluster 4, and discuss the structures in the context of structures from serologically and genetically related serotypes.
25036733	7	60	theme	typing	1426:1431	arg1	35a					1439:1441	the typing serum 35a	1422:1441	the typing serum 35a	1422:1441	The structure of the serotype 47F capsular polysaccharide [formula: see text] is somewhat different from a recently determined structure from the same serogroup, while containing a structural motif that is reflected in serotype 35A and 42 capsular polysaccharide structures, thus explaining the cross-reactivity of serotype 47F with the typing serum 35a.
25036733	5	61	theme	capsular	896:903	arg1	polysaccharide					905:918	the capsular polysaccharide 10A, but two different linkages	892:950	polysaccharide	905:918	The structure of the serotype 39 capsular polysaccharide is [formula: see text] and has identical composition to the capsular polysaccharide 10A, but two different linkages.
25036733	1	62	theme	pathogen	236:243	arg1	pneumoniae					259:268	the bacterial pathogen Streptococcus pneumoniae	222:268	the bacterial pathogen Streptococcus pneumoniae	222:268	The diversity of capsular polysaccharides of the bacterial pathogen Streptococcus pneumoniae leads to at least 91 different serotypes.
25036733	0	63	theme	serotypes	87:95	arg1	structures					48:57	native capsular polysaccharide structures	17:57	native capsular polysaccharide structures of Streptococcus pneumoniae serotypes 39, 42, and 47F	17:111	Determination of native capsular polysaccharide structures of Streptococcus pneumoniae serotypes 39, 42, and 47F and comparison to genetically or serologically related strains.
25036733	6	64	dep	[formula	979:986	arg1	see					989:991	see	989:991	see text	989:996	The serotype 42 structure [formula: see text] closely resembles the genetically related serotype 35A, which does not contain residue A.
25036733	1	65	theme	capsular	194:201	arg1	polysaccharides					203:217	capsular polysaccharides	194:217	capsular polysaccharides of the bacterial pathogen Streptococcus pneumoniae	194:268	The diversity of capsular polysaccharides of the bacterial pathogen Streptococcus pneumoniae leads to at least 91 different serotypes.
25036733	1	66	theme	Streptococcus	245:257	arg1	pneumoniae					259:268	the bacterial pathogen Streptococcus pneumoniae	222:268	the bacterial pathogen Streptococcus pneumoniae	222:268	The diversity of capsular polysaccharides of the bacterial pathogen Streptococcus pneumoniae leads to at least 91 different serotypes.
25036733	3	67	from	serotypes	709:717	arg1	context					644:650	the context	640:650	the context of structures from serologically and genetically related serotypes	640:717	Here, we report the chemical structures of the capsular polysaccharides of serotypes 39, 42, and 47F from the genetic cluster 4, and discuss the structures in the context of structures from serologically and genetically related serotypes.
25036733	3	67	from	serotypes	709:717	arg1	structures					655:664	structures	655:664	structures from serologically and genetically related serotypes	655:717	Here, we report the chemical structures of the capsular polysaccharides of serotypes 39, 42, and 47F from the genetic cluster 4, and discuss the structures in the context of structures from serologically and genetically related serotypes.
25036733	7	68	theme	capsular	1328:1335	arg1	structures					1352:1361	42 capsular polysaccharide structures	1325:1361	42 capsular polysaccharide structures	1325:1361	The structure of the serotype 47F capsular polysaccharide [formula: see text] is somewhat different from a recently determined structure from the same serogroup, while containing a structural motif that is reflected in serotype 35A and 42 capsular polysaccharide structures, thus explaining the cross-reactivity of serotype 47F with the typing serum 35a.
25036733	3	69	theme	serotypes	556:564	arg1	polysaccharides					537:551	the capsular polysaccharides	524:551	the capsular polysaccharides of serotypes 39, 42, and 47F from the genetic cluster 4	524:607	Here, we report the chemical structures of the capsular polysaccharides of serotypes 39, 42, and 47F from the genetic cluster 4, and discuss the structures in the context of structures from serologically and genetically related serotypes.
25036733	1	70	theme	pneumoniae	259:268	arg1	polysaccharides					203:217	capsular polysaccharides	194:217	capsular polysaccharides of the bacterial pathogen Streptococcus pneumoniae	194:268	The diversity of capsular polysaccharides of the bacterial pathogen Streptococcus pneumoniae leads to at least 91 different serotypes.
25326173	4	0	theme	traditional	517:527	arg1	wax					529:531	traditional wax	517:531	traditional wax	517:531	Here, a bioactive glass/chitosan/carboxymethyl cellulose (BG/CS/CMC) composite scaffold was designed to replace traditional wax.
25326173	5	1	theme	BG/CS/CMC	560:568	arg1	composite					570:578	the BG/CS/CMC composite	556:578	the BG/CS/CMC composite resulted in excellent biocompatibility with no cytotoxicity	556:638	WST-1 assay indicated the BG/CS/CMC composite resulted in excellent biocompatibility with no cytotoxicity.
25326173	6	2	theme	dominant	720:727	arg1	role					729:732	a dominant role	718:732	a dominant role	718:732	In vivo osteogenesis assessment revealed that the BG/CS/CMC composite played a dominant role in bone regeneration and hemostasis.
25326173	0	3	theme	bone	105:108	arg1	regeneration					110:121	bone regeneration	105:121	bone regeneration	105:121	Biodegradable composite scaffolds of bioactive glass/chitosan/carboxymethyl cellulose for hemostatic and bone regeneration.
25326173	7	4	theme	defects	920:926	arg1	reconstruction					897:910	the functional reconstruction	882:910	the functional reconstruction of bone defects	882:926	The BG/CS/CMC composite had the same hemostasis effect as bone wax; in addition its biodegradation also led to the functional reconstruction of bone defects.
25326173	3	5	theme	foreign	365:371	arg1	reaction					380:387	foreign matter reaction	365:387	foreign matter reaction	365:387	However, the wax cannot be absorbed, which leads to artificial prosthesis loosening, foreign matter reaction, and infection.
25326173	3	6	theme	matter	373:378	arg1	reaction					380:387	foreign matter reaction	365:387	foreign matter reaction	365:387	However, the wax cannot be absorbed, which leads to artificial prosthesis loosening, foreign matter reaction, and infection.
25326173	8	7	theme	bone	1038:1041	arg1	defects					1043:1049	critical-sized bone defects	1023:1049	critical-sized bone defects	1023:1049	Thus, BG/CS/CMC scaffolds can serve as a potential material for bone repair and hemostasis in critical-sized bone defects.
25326173	6	8	theme	osteogenesis	649:660	arg1	assessment					662:671	In vivo osteogenesis assessment	641:671	In vivo osteogenesis assessment	641:671	In vivo osteogenesis assessment revealed that the BG/CS/CMC composite played a dominant role in bone regeneration and hemostasis.
25326173	7	9	theme	BG/CS/CMC	775:783	arg1	composite					785:793	The BG/CS/CMC composite	771:793	The BG/CS/CMC composite	771:793	The BG/CS/CMC composite had the same hemostasis effect as bone wax; in addition its biodegradation also led to the functional reconstruction of bone defects.
25326173	8	10	theme	critical-sized	1023:1036	arg1	defects					1043:1049	critical-sized bone defects	1023:1049	critical-sized bone defects	1023:1049	Thus, BG/CS/CMC scaffolds can serve as a potential material for bone repair and hemostasis in critical-sized bone defects.
25326173	7	11	theme	bone	915:918	arg1	defects					920:926	bone defects	915:926	bone defects	915:926	The BG/CS/CMC composite had the same hemostasis effect as bone wax; in addition its biodegradation also led to the functional reconstruction of bone defects.
25326173	7	12	theme	functional	886:895	arg1	reconstruction					897:910	the functional reconstruction	882:910	the functional reconstruction of bone defects	882:926	The BG/CS/CMC composite had the same hemostasis effect as bone wax; in addition its biodegradation also led to the functional reconstruction of bone defects.
25326173	0	13	theme	composite	14:22	arg1	scaffolds					24:32	Biodegradable composite scaffolds	0:32	Biodegradable composite scaffolds of bioactive glass/chitosan/carboxymethyl cellulose for hemostatic and bone regeneration.	0:122	Biodegradable composite scaffolds of bioactive glass/chitosan/carboxymethyl cellulose for hemostatic and bone regeneration.
25326173	5	14	theme	WST-1	534:538	arg1	assay					540:544	WST-1 assay	534:544	WST-1 assay	534:544	WST-1 assay indicated the BG/CS/CMC composite resulted in excellent biocompatibility with no cytotoxicity.
25326173	0	15	theme	Biodegradable	0:12	arg1	scaffolds					24:32	Biodegradable composite scaffolds	0:32	Biodegradable composite scaffolds of bioactive glass/chitosan/carboxymethyl cellulose for hemostatic and bone regeneration.	0:122	Biodegradable composite scaffolds of bioactive glass/chitosan/carboxymethyl cellulose for hemostatic and bone regeneration.
25326173	5	16	theme	no	624:625	arg1	cytotoxicity					627:638	no cytotoxicity	624:638	no cytotoxicity	624:638	WST-1 assay indicated the BG/CS/CMC composite resulted in excellent biocompatibility with no cytotoxicity.
25326173	6	17	theme	bone	737:740	arg1	regeneration					742:753	bone regeneration	737:753	bone regeneration	737:753	In vivo osteogenesis assessment revealed that the BG/CS/CMC composite played a dominant role in bone regeneration and hemostasis.
25326173	6	18	theme	In	641:642	arg1	assessment					662:671	In vivo osteogenesis assessment	641:671	In vivo osteogenesis assessment	641:671	In vivo osteogenesis assessment revealed that the BG/CS/CMC composite played a dominant role in bone regeneration and hemostasis.
25326173	8	19	theme	BG/CS/CMC	935:943	arg1	scaffolds					945:953	BG/CS/CMC scaffolds	935:953	BG/CS/CMC scaffolds	935:953	Thus, BG/CS/CMC scaffolds can serve as a potential material for bone repair and hemostasis in critical-sized bone defects.
25326173	8	19	theme	BG/CS/CMC	935:943	arg1	material					980:987	a potential material	968:987	a potential material for bone repair and hemostasis in critical-sized bone defects	968:1049	Thus, BG/CS/CMC scaffolds can serve as a potential material for bone repair and hemostasis in critical-sized bone defects.
25326173	8	20	from	hemostasis	1009:1018	arg1	defects					1043:1049	critical-sized bone defects	1023:1049	critical-sized bone defects	1023:1049	Thus, BG/CS/CMC scaffolds can serve as a potential material for bone repair and hemostasis in critical-sized bone defects.
25326173	5	21	with	biocompatibility	602:617	arg1	cytotoxicity					627:638	no cytotoxicity	624:638	no cytotoxicity	624:638	WST-1 assay indicated the BG/CS/CMC composite resulted in excellent biocompatibility with no cytotoxicity.
25326173	1	22	theme	orthopedic	138:147	arg1	osteotomy					149:157	orthopedic osteotomy	138:157	orthopedic osteotomy	138:157	Hemostasis in orthopedic osteotomy or bone cutting requires different methods and materials.
25326173	8	23	from	repair	998:1003	arg1	defects					1043:1049	critical-sized bone defects	1023:1049	critical-sized bone defects	1023:1049	Thus, BG/CS/CMC scaffolds can serve as a potential material for bone repair and hemostasis in critical-sized bone defects.
25326173	0	24	theme	glass/chitosan/carboxymethyl	47:74	arg1	cellulose					76:84	bioactive glass/chitosan/carboxymethyl cellulose	37:84	bioactive glass/chitosan/carboxymethyl cellulose	37:84	Biodegradable composite scaffolds of bioactive glass/chitosan/carboxymethyl cellulose for hemostatic and bone regeneration.
25326173	4	25	theme	BG/CS/CMC	463:471	arg1	scaffold					484:491	a bioactive glass/chitosan/carboxymethyl cellulose (BG/CS/CMC) composite scaffold	411:491	a bioactive glass/chitosan/carboxymethyl cellulose (BG/CS/CMC) composite scaffold	411:491	Here, a bioactive glass/chitosan/carboxymethyl cellulose (BG/CS/CMC) composite scaffold was designed to replace traditional wax.
25326173	6	26	theme	BG/CS/CMC	691:699	arg1	composite					701:709	the BG/CS/CMC composite	687:709	the BG/CS/CMC composite	687:709	In vivo osteogenesis assessment revealed that the BG/CS/CMC composite played a dominant role in bone regeneration and hemostasis.
25326173	0	27	theme	bioactive	37:45	arg1	cellulose					76:84	bioactive glass/chitosan/carboxymethyl cellulose	37:84	bioactive glass/chitosan/carboxymethyl cellulose	37:84	Biodegradable composite scaffolds of bioactive glass/chitosan/carboxymethyl cellulose for hemostatic and bone regeneration.
25326173	6	28	dep	In	641:642	arg1	vivo					644:647	vivo	644:647	vivo	644:647	In vivo osteogenesis assessment revealed that the BG/CS/CMC composite played a dominant role in bone regeneration and hemostasis.
25326173	4	29	theme	composite	474:482	arg1	scaffold					484:491	a bioactive glass/chitosan/carboxymethyl cellulose (BG/CS/CMC) composite scaffold	411:491	a bioactive glass/chitosan/carboxymethyl cellulose (BG/CS/CMC) composite scaffold	411:491	Here, a bioactive glass/chitosan/carboxymethyl cellulose (BG/CS/CMC) composite scaffold was designed to replace traditional wax.
25326173	7	30	contain	had	795:797	arg1	composite					785:793	The BG/CS/CMC composite	771:793	The BG/CS/CMC composite	771:793	The BG/CS/CMC composite had the same hemostasis effect as bone wax; in addition its biodegradation also led to the functional reconstruction of bone defects.
25326173	7	30	contain	had	795:797	arg2	effect					819:824	the same hemostasis effect	799:824	the same hemostasis effect	799:824	The BG/CS/CMC composite had the same hemostasis effect as bone wax; in addition its biodegradation also led to the functional reconstruction of bone defects.
25326173	0	31	theme	cellulose	76:84	arg1	scaffolds					24:32	Biodegradable composite scaffolds	0:32	Biodegradable composite scaffolds of bioactive glass/chitosan/carboxymethyl cellulose for hemostatic and bone regeneration.	0:122	Biodegradable composite scaffolds of bioactive glass/chitosan/carboxymethyl cellulose for hemostatic and bone regeneration.
25326173	4	32	theme	glass/chitosan/carboxymethyl	423:450	arg1	scaffold					484:491	a bioactive glass/chitosan/carboxymethyl cellulose (BG/CS/CMC) composite scaffold	411:491	a bioactive glass/chitosan/carboxymethyl cellulose (BG/CS/CMC) composite scaffold	411:491	Here, a bioactive glass/chitosan/carboxymethyl cellulose (BG/CS/CMC) composite scaffold was designed to replace traditional wax.
25326173	4	33	theme	bioactive	413:421	arg1	scaffold					484:491	a bioactive glass/chitosan/carboxymethyl cellulose (BG/CS/CMC) composite scaffold	411:491	a bioactive glass/chitosan/carboxymethyl cellulose (BG/CS/CMC) composite scaffold	411:491	Here, a bioactive glass/chitosan/carboxymethyl cellulose (BG/CS/CMC) composite scaffold was designed to replace traditional wax.
25326173	7	34	theme	bone	829:832	arg1	wax					834:836	bone wax	829:836	bone wax	829:836	The BG/CS/CMC composite had the same hemostasis effect as bone wax; in addition its biodegradation also led to the functional reconstruction of bone defects.
25326173	2	35	theme	marrow	238:243	arg1	bleeding					221:228	bleeding	221:228	bleeding	221:228	The bleeding of bone marrow can be mostly stopped by bone wax.
25326173	5	36	theme	excellent	592:600	arg1	biocompatibility					602:617	excellent biocompatibility	592:617	excellent biocompatibility with no cytotoxicity	592:638	WST-1 assay indicated the BG/CS/CMC composite resulted in excellent biocompatibility with no cytotoxicity.
25326173	7	37	theme	hemostasis	808:817	arg1	effect					819:824	the same hemostasis effect	799:824	the same hemostasis effect	799:824	The BG/CS/CMC composite had the same hemostasis effect as bone wax; in addition its biodegradation also led to the functional reconstruction of bone defects.
25326173	8	38	theme	potential	970:978	arg1	scaffolds					945:953	BG/CS/CMC scaffolds	935:953	BG/CS/CMC scaffolds	935:953	Thus, BG/CS/CMC scaffolds can serve as a potential material for bone repair and hemostasis in critical-sized bone defects.
25326173	8	38	theme	potential	970:978	arg1	material					980:987	a potential material	968:987	a potential material for bone repair and hemostasis in critical-sized bone defects	968:1049	Thus, BG/CS/CMC scaffolds can serve as a potential material for bone repair and hemostasis in critical-sized bone defects.
25326173	2	39	theme	bone	233:236	arg1	marrow					238:243	bone marrow	233:243	bone marrow	233:243	The bleeding of bone marrow can be mostly stopped by bone wax.
25326173	8	40	theme	bone	993:996	arg1	repair					998:1003	bone repair	993:1003	bone repair	993:1003	Thus, BG/CS/CMC scaffolds can serve as a potential material for bone repair and hemostasis in critical-sized bone defects.
25326173	1	41	theme	cutting	167:173	arg1	bone					162:165	bone cutting	162:173	bone cutting	162:173	Hemostasis in orthopedic osteotomy or bone cutting requires different methods and materials.
25326173	3	42	theme	artificial	332:341	arg1	loosening					354:362	artificial prosthesis loosening	332:362	artificial prosthesis loosening	332:362	However, the wax cannot be absorbed, which leads to artificial prosthesis loosening, foreign matter reaction, and infection.
25326173	4	43	theme	cellulose	452:460	arg1	scaffold					484:491	a bioactive glass/chitosan/carboxymethyl cellulose (BG/CS/CMC) composite scaffold	411:491	a bioactive glass/chitosan/carboxymethyl cellulose (BG/CS/CMC) composite scaffold	411:491	Here, a bioactive glass/chitosan/carboxymethyl cellulose (BG/CS/CMC) composite scaffold was designed to replace traditional wax.
25326173	1	44	from	Hemostasis	124:133	arg1	bone					162:165	bone cutting	162:173	bone cutting	162:173	Hemostasis in orthopedic osteotomy or bone cutting requires different methods and materials.
25326173	1	44	from	Hemostasis	124:133	arg1	osteotomy					149:157	orthopedic osteotomy	138:157	orthopedic osteotomy	138:157	Hemostasis in orthopedic osteotomy or bone cutting requires different methods and materials.
25326173	3	45	theme	prosthesis	343:352	arg1	loosening					354:362	artificial prosthesis loosening	332:362	artificial prosthesis loosening	332:362	However, the wax cannot be absorbed, which leads to artificial prosthesis loosening, foreign matter reaction, and infection.
25326173	2	46	theme	bone	270:273	arg1	wax					275:277	bone wax	270:277	bone wax	270:277	The bleeding of bone marrow can be mostly stopped by bone wax.
25326173	1	47	theme	different	184:192	arg1	methods					194:200	methods	194:200	methods	194:200	Hemostasis in orthopedic osteotomy or bone cutting requires different methods and materials.
25326173	7	48	theme	same	803:806	arg1	effect					819:824	the same hemostasis effect	799:824	the same hemostasis effect	799:824	The BG/CS/CMC composite had the same hemostasis effect as bone wax; in addition its biodegradation also led to the functional reconstruction of bone defects.
27842855	8	0	used	used	929:932	arg2	types					886:890	Both types	881:890	Both types of agricultural by-products	881:918	Both types of agricultural by-products could be used for preparation of composite film with high strength and stiffness suitable for broad range of applications.
27842855	7	1	theme	Lignin	760:765	arg1	content					767:773	Lignin content	760:773	Lignin content of the sugar beet holocellulose and bagasse samples	760:825	Lignin content of the sugar beet holocellulose and bagasse samples was low and did not affect the mechanical properties.
27842855	2	2	from	holocellulose	164:176	arg1	Films					142:146	Films	142:146	Films from sugar beet holocellulose	142:176	Films from sugar beet holocellulose have better mechanical properties than from bagasse holocellulose.
27842855	7	3	dep	beet	788:791	arg1	holocellulose					793:805	holocellulose	793:805	holocellulose	793:805	Lignin content of the sugar beet holocellulose and bagasse samples was low and did not affect the mechanical properties.
27842855	6	4	theme	microwaves	646:655	arg1	use					639:641	The use	635:641	The use of microwaves to prepare holocellulose film	635:685	The use of microwaves to prepare holocellulose film speed up the process, but negatively affected the mechanical properties.
27842855	8	5	theme	suitable	1001:1008	arg1	strength					978:985	high strength	973:985	high strength	973:985	Both types of agricultural by-products could be used for preparation of composite film with high strength and stiffness suitable for broad range of applications.
27842855	8	6	theme	film	963:966	arg1	preparation					938:948	preparation	938:948	preparation of composite film with high strength and stiffness suitable for broad range of applications	938:1040	Both types of agricultural by-products could be used for preparation of composite film with high strength and stiffness suitable for broad range of applications.
27842855	4	7	theme	mechanical	449:458	arg1	properties					460:469	the best mechanical properties	440:469	the best mechanical properties	440:469	Specimens prepared from combined sugar beet and bagasse carboxymethylated holocellulose had the best mechanical properties.
27842855	6	8	theme	mechanical	737:746	arg1	properties					748:757	the mechanical properties	733:757	the mechanical properties	733:757	The use of microwaves to prepare holocellulose film speed up the process, but negatively affected the mechanical properties.
27842855	8	9	theme	by-products	908:918	arg1	types					886:890	Both types	881:890	Both types of agricultural by-products	881:918	Both types of agricultural by-products could be used for preparation of composite film with high strength and stiffness suitable for broad range of applications.
27842855	5	10	theme	analysis	620:627	arg1	data					629:632	the elemental analysis data	606:632	the elemental analysis data	606:632	The results could be explained by the ratios of cellulose, arabinan, polygalacturonan and xylan content in individual films, based on the elemental analysis data.
27842855	8	11	with	preparation	938:948	arg1	strength					978:985	high strength	973:985	high strength	973:985	Both types of agricultural by-products could be used for preparation of composite film with high strength and stiffness suitable for broad range of applications.
27842855	8	11	with	preparation	938:948	arg1	stiffness					991:999	stiffness	991:999	stiffness	991:999	Both types of agricultural by-products could be used for preparation of composite film with high strength and stiffness suitable for broad range of applications.
27842855	4	12	theme	best	444:447	arg1	properties					460:469	the best mechanical properties	440:469	the best mechanical properties	440:469	Specimens prepared from combined sugar beet and bagasse carboxymethylated holocellulose had the best mechanical properties.
27842855	5	13	theme	arabinan	531:538	arg1	content					568:574	cellulose, arabinan, polygalacturonan and xylan content	520:574	cellulose, arabinan, polygalacturonan and xylan content in individual films	520:594	The results could be explained by the ratios of cellulose, arabinan, polygalacturonan and xylan content in individual films, based on the elemental analysis data.
27842855	5	14	from	content	568:574	arg1	films					590:594	individual films	579:594	individual films	579:594	The results could be explained by the ratios of cellulose, arabinan, polygalacturonan and xylan content in individual films, based on the elemental analysis data.
27842855	4	15	theme	bagasse	396:402	arg1	holocellulose					422:434	bagasse carboxymethylated holocellulose	396:434	bagasse carboxymethylated holocellulose	396:434	Specimens prepared from combined sugar beet and bagasse carboxymethylated holocellulose had the best mechanical properties.
27842855	8	16	theme	agricultural	895:906	arg1	by-products					908:918	agricultural by-products	895:918	agricultural by-products	895:918	Both types of agricultural by-products could be used for preparation of composite film with high strength and stiffness suitable for broad range of applications.
27842855	5	17	theme	polygalacturonan	541:556	arg1	content					568:574	cellulose, arabinan, polygalacturonan and xylan content	520:574	cellulose, arabinan, polygalacturonan and xylan content in individual films	520:594	The results could be explained by the ratios of cellulose, arabinan, polygalacturonan and xylan content in individual films, based on the elemental analysis data.
27842855	0	18	theme	Composite	0:8	arg1	films					10:14	Composite films	0:14	Composite films	0:14	Composite films prepared from agricultural by-products.
27842855	3	19	theme	subsequent	248:257	arg1	carboxymethylation					259:276	subsequent carboxymethylation	248:276	subsequent carboxymethylation of bagasse holocellulose	248:301	By subsequent carboxymethylation of bagasse holocellulose, films with better properties were produced.
27842855	5	20	theme	elemental	610:618	arg1	data					629:632	the elemental analysis data	606:632	the elemental analysis data	606:632	The results could be explained by the ratios of cellulose, arabinan, polygalacturonan and xylan content in individual films, based on the elemental analysis data.
27842855	2	21	contain	have	178:181	arg2	properties					201:210	better mechanical properties	183:210	better mechanical properties	183:210	Films from sugar beet holocellulose have better mechanical properties than from bagasse holocellulose.
27842855	2	21	contain	have	178:181	arg1	Films					142:146	Films	142:146	Films from sugar beet holocellulose	142:176	Films from sugar beet holocellulose have better mechanical properties than from bagasse holocellulose.
27842855	8	22	theme	composite	953:961	arg1	film					963:966	composite film	953:966	composite film	953:966	Both types of agricultural by-products could be used for preparation of composite film with high strength and stiffness suitable for broad range of applications.
27842855	1	23	from	bagasse	112:118	arg1	holocelluloses					77:90	holocelluloses	77:90	holocelluloses from sugar beet and bagasse for film preparation	77:139	In our study we used holocelluloses from sugar beet and bagasse for film preparation.
27842855	1	24	theme	sugar	97:101	arg1	beet					103:106	sugar beet	97:106	sugar beet	97:106	In our study we used holocelluloses from sugar beet and bagasse for film preparation.
27842855	7	25	theme	mechanical	858:867	arg1	properties					869:878	the mechanical properties	854:878	the mechanical properties	854:878	Lignin content of the sugar beet holocellulose and bagasse samples was low and did not affect the mechanical properties.
27842855	8	26	theme	high	973:976	arg1	strength					978:985	high strength	973:985	high strength	973:985	Both types of agricultural by-products could be used for preparation of composite film with high strength and stiffness suitable for broad range of applications.
27842855	3	27	theme	bagasse	281:287	arg1	holocellulose					289:301	bagasse holocellulose	281:301	bagasse holocellulose	281:301	By subsequent carboxymethylation of bagasse holocellulose, films with better properties were produced.
27842855	5	28	theme	cellulose	520:528	arg1	content					568:574	cellulose, arabinan, polygalacturonan and xylan content	520:574	cellulose, arabinan, polygalacturonan and xylan content in individual films	520:594	The results could be explained by the ratios of cellulose, arabinan, polygalacturonan and xylan content in individual films, based on the elemental analysis data.
27842855	0	29	theme	agricultural	30:41	arg1	by-products					43:53	agricultural by-products	30:53	agricultural by-products	30:53	Composite films prepared from agricultural by-products.
27842855	7	30	theme	bagasse	811:817	arg1	samples					819:825	bagasse samples	811:825	bagasse samples	811:825	Lignin content of the sugar beet holocellulose and bagasse samples was low and did not affect the mechanical properties.
27842855	3	31	theme	holocellulose	289:301	arg1	carboxymethylation					259:276	subsequent carboxymethylation	248:276	subsequent carboxymethylation of bagasse holocellulose	248:301	By subsequent carboxymethylation of bagasse holocellulose, films with better properties were produced.
27842855	2	32	theme	beet	159:162	arg1	holocellulose					164:176	sugar beet holocellulose	153:176	sugar beet holocellulose	153:176	Films from sugar beet holocellulose have better mechanical properties than from bagasse holocellulose.
27842855	4	33	theme	combined	372:379	arg1	beet					387:390	combined sugar beet	372:390	combined sugar beet	372:390	Specimens prepared from combined sugar beet and bagasse carboxymethylated holocellulose had the best mechanical properties.
27842855	3	34	with	films	304:308	arg1	properties					322:331	better properties	315:331	better properties	315:331	By subsequent carboxymethylation of bagasse holocellulose, films with better properties were produced.
27842855	4	35	contain	had	436:438	arg1	Specimens					348:356	Specimens	348:356	Specimens prepared from combined sugar beet and bagasse carboxymethylated holocellulose	348:434	Specimens prepared from combined sugar beet and bagasse carboxymethylated holocellulose had the best mechanical properties.
27842855	4	35	contain	had	436:438	arg2	properties					460:469	the best mechanical properties	440:469	the best mechanical properties	440:469	Specimens prepared from combined sugar beet and bagasse carboxymethylated holocellulose had the best mechanical properties.
27842855	7	36	theme	samples	819:825	arg1	content					767:773	Lignin content	760:773	Lignin content of the sugar beet holocellulose and bagasse samples	760:825	Lignin content of the sugar beet holocellulose and bagasse samples was low and did not affect the mechanical properties.
27842855	2	37	theme	sugar	153:157	arg1	holocellulose					164:176	sugar beet holocellulose	153:176	sugar beet holocellulose	153:176	Films from sugar beet holocellulose have better mechanical properties than from bagasse holocellulose.
27842855	2	38	theme	bagasse	222:228	arg1	holocellulose					230:242	bagasse holocellulose	222:242	bagasse holocellulose	222:242	Films from sugar beet holocellulose have better mechanical properties than from bagasse holocellulose.
27842855	5	39	from	ratios	510:515	arg1	films					590:594	individual films	579:594	individual films	579:594	The results could be explained by the ratios of cellulose, arabinan, polygalacturonan and xylan content in individual films, based on the elemental analysis data.
27842855	4	40	theme	carboxymethylated	404:420	arg1	holocellulose					422:434	bagasse carboxymethylated holocellulose	396:434	bagasse carboxymethylated holocellulose	396:434	Specimens prepared from combined sugar beet and bagasse carboxymethylated holocellulose had the best mechanical properties.
27842855	8	41	theme	applications	1029:1040	arg1	range					1020:1024	broad range	1014:1024	broad range of applications	1014:1040	Both types of agricultural by-products could be used for preparation of composite film with high strength and stiffness suitable for broad range of applications.
27842855	4	42	theme	sugar	381:385	arg1	beet					387:390	combined sugar beet	372:390	combined sugar beet	372:390	Specimens prepared from combined sugar beet and bagasse carboxymethylated holocellulose had the best mechanical properties.
27842855	8	43	theme	broad	1014:1018	arg1	range					1020:1024	broad range	1014:1024	broad range of applications	1014:1040	Both types of agricultural by-products could be used for preparation of composite film with high strength and stiffness suitable for broad range of applications.
27842855	1	44	from	beet	103:106	arg1	holocelluloses					77:90	holocelluloses	77:90	holocelluloses from sugar beet and bagasse for film preparation	77:139	In our study we used holocelluloses from sugar beet and bagasse for film preparation.
27842855	7	45	theme	sugar	782:786	arg1	beet					788:791	the sugar beet holocellulose and bagasse samples	778:825	beet	788:791	Lignin content of the sugar beet holocellulose and bagasse samples was low and did not affect the mechanical properties.
27842855	3	46	theme	better	315:320	arg1	properties					322:331	better properties	315:331	better properties	315:331	By subsequent carboxymethylation of bagasse holocellulose, films with better properties were produced.
27842855	2	47	theme	mechanical	190:199	arg1	properties					201:210	better mechanical properties	183:210	better mechanical properties	183:210	Films from sugar beet holocellulose have better mechanical properties than from bagasse holocellulose.
27842855	5	48	theme	individual	579:588	arg1	films					590:594	individual films	579:594	individual films	579:594	The results could be explained by the ratios of cellulose, arabinan, polygalacturonan and xylan content in individual films, based on the elemental analysis data.
27842855	1	49	theme	film	124:127	arg1	preparation					129:139	film preparation	124:139	film preparation	124:139	In our study we used holocelluloses from sugar beet and bagasse for film preparation.
27842855	7	50	theme	beet	788:791	arg1	content					767:773	Lignin content	760:773	Lignin content of the sugar beet holocellulose and bagasse samples	760:825	Lignin content of the sugar beet holocellulose and bagasse samples was low and did not affect the mechanical properties.
27842855	5	51	from	films	590:594	arg1	ratios					510:515	the ratios	506:515	the ratios of cellulose, arabinan, polygalacturonan and xylan content in individual films	506:594	The results could be explained by the ratios of cellulose, arabinan, polygalacturonan and xylan content in individual films, based on the elemental analysis data.
27842855	2	52	theme	better	183:188	arg1	properties					201:210	better mechanical properties	183:210	better mechanical properties	183:210	Films from sugar beet holocellulose have better mechanical properties than from bagasse holocellulose.
27842855	5	53	theme	xylan	562:566	arg1	content					568:574	cellulose, arabinan, polygalacturonan and xylan content	520:574	cellulose, arabinan, polygalacturonan and xylan content in individual films	520:594	The results could be explained by the ratios of cellulose, arabinan, polygalacturonan and xylan content in individual films, based on the elemental analysis data.
27842855	1	54	used	used	72:75	arg2	we					69:70	we	69:70	we	69:70	In our study we used holocelluloses from sugar beet and bagasse for film preparation.
27842855	6	55	theme	holocellulose	668:680	arg1	film					682:685	holocellulose film	668:685	holocellulose film	668:685	The use of microwaves to prepare holocellulose film speed up the process, but negatively affected the mechanical properties.
27842855	5	56	theme	content	568:574	arg1	ratios					510:515	the ratios	506:515	the ratios of cellulose, arabinan, polygalacturonan and xylan content in individual films	506:594	The results could be explained by the ratios of cellulose, arabinan, polygalacturonan and xylan content in individual films, based on the elemental analysis data.
28420283	0	0	theme	meloxicam	71:79	arg1	release					60:66	modified release	51:66	modified release of meloxicam	51:79	Design and characteristics of gellan gum beads for modified release of meloxicam.
28420283	5	1	theme	modulus	679:685	arg1	Design					651:656	the Design	647:656	the Design of Experiments (DoE) modulus from the STATISTICA Software	647:714	The quantitative compositions of the mixtures were generated with the application of the Design of Experiments (DoE) modulus from the STATISTICA Software.
28420283	5	2	theme	quantitative	566:577	arg1	compositions					579:590	The quantitative compositions	562:590	The quantitative compositions of the mixtures	562:606	The quantitative compositions of the mixtures were generated with the application of the Design of Experiments (DoE) modulus from the STATISTICA Software.
28420283	6	3	theme	drug	793:796	arg1	efficiency					812:821	29.2-50.7% drug encapsulation efficiency	782:821	29.2-50.7% drug encapsulation efficiency	782:821	The prepared formulations revealed 7.2-27.0% of drug loading and 29.2-50.7% drug encapsulation efficiency.
28420283	10	4	theme	swelling	1103:1110	arg1	behavior					1112:1119	The swelling behavior	1099:1119	The swelling behavior	1099:1119	The swelling behavior and degradation of the beads were evaluated in three simulated gastrointestinal fluids at different pH (1.2; 4.5; 6.8).
28420283	11	5	theme	MLX	1245:1247	arg1	release					1258:1264	The MLX in vitro release	1241:1264	The MLX in vitro release studies	1241:1272	The MLX in vitro release studies were conducted on USP apparatus IV, working in the open loop mode.
28420283	13	6	theme	gastrointestinal	1624:1639	arg1	tract					1641:1645	the gastrointestinal tract	1620:1645	the gastrointestinal tract	1620:1645	The formulations obtained from mixtures containing 1.0 and 1.5% of gellan may be considered as oral dosage forms for MLX, intended to omit the stomach and release the drug in the distal parts of the gastrointestinal tract.
28420283	12	7	from	beads	1401:1405	arg1	release					1378:1384	MLX release	1374:1384	MLX release from the dried beads	1374:1405	The obtained results showed that MLX release from the dried beads was pH-dependent.
28420283	3	8	theme	cross-linking	438:450	arg1	chloride					392:399	Calcium chloride	384:399	Calcium chloride (1.0%, 9.0 × 10-2 M)	384:420	Calcium chloride (1.0%, 9.0 × 10-2 M) was used as the cross-linking agent.
28420283	3	8	theme	cross-linking	438:450	arg1	agent					452:456	the cross-linking agent	434:456	the cross-linking agent	434:456	Calcium chloride (1.0%, 9.0 × 10-2 M) was used as the cross-linking agent.
28420283	10	9	theme	beads	1144:1148	arg1	degradation					1125:1135	degradation	1125:1135	degradation of the beads	1125:1148	The swelling behavior and degradation of the beads were evaluated in three simulated gastrointestinal fluids at different pH (1.2; 4.5; 6.8).
28420283	10	9	theme	beads	1144:1148	arg1	behavior					1112:1119	The swelling behavior	1099:1119	The swelling behavior	1099:1119	The swelling behavior and degradation of the beads were evaluated in three simulated gastrointestinal fluids at different pH (1.2; 4.5; 6.8).
28420283	12	10	theme	obtained	1345:1352	arg1	results					1354:1360	The obtained results	1341:1360	The obtained results	1341:1360	The obtained results showed that MLX release from the dried beads was pH-dependent.
28420283	5	11	theme	Design	651:656	arg1	application					632:642	the application	628:642	the application of the Design of Experiments (DoE) modulus from the STATISTICA Software	628:714	The quantitative compositions of the mixtures were generated with the application of the Design of Experiments (DoE) modulus from the STATISTICA Software.
28420283	1	12	theme	colorectal	298:307	arg1	cancer					309:314	colorectal cancer	298:314	colorectal cancer	298:314	The aim of the presented work was to design, formulate and evaluate the properties of low-acyl gellan macro beads with the potential application as carriers for oral delivery of meloxicam (MLX) in the prophylaxis of colorectal cancer.
28420283	6	13	theme	29.2-50.7	782:790	arg1	%					791:791	%	791:791	%	791:791	The prepared formulations revealed 7.2-27.0% of drug loading and 29.2-50.7% drug encapsulation efficiency.
28420283	8	14	dep	morphology	935:944	arg1	The					931:933	The	931:933	The	931:933	The morphology and surface of the dried beads were analyzed by SEM.
28420283	1	15	theme	cancer	309:314	arg1	prophylaxis					283:293	the prophylaxis	279:293	the prophylaxis of colorectal cancer	279:314	The aim of the presented work was to design, formulate and evaluate the properties of low-acyl gellan macro beads with the potential application as carriers for oral delivery of meloxicam (MLX) in the prophylaxis of colorectal cancer.
28420283	6	16	theme	prepared	721:728	arg1	formulations					730:741	The prepared formulations	717:741	The prepared formulations	717:741	The prepared formulations revealed 7.2-27.0% of drug loading and 29.2-50.7% drug encapsulation efficiency.
28420283	11	17	theme	in	1249:1250	arg1	release					1258:1264	The MLX in vitro release	1241:1264	The MLX in vitro release studies	1241:1272	The MLX in vitro release studies were conducted on USP apparatus IV, working in the open loop mode.
28420283	10	18	theme	gastrointestinal	1184:1199	arg1	fluids					1201:1206	three simulated gastrointestinal fluids	1168:1206	three simulated gastrointestinal fluids at different pH (1.2; 4.5; 6.8)	1168:1238	The swelling behavior and degradation of the beads were evaluated in three simulated gastrointestinal fluids at different pH (1.2; 4.5; 6.8).
28420283	5	19	theme	Experiments	661:671	arg1	modulus					679:685	Experiments (DoE) modulus	661:685	Experiments (DoE) modulus from the STATISTICA Software	661:714	The quantitative compositions of the mixtures were generated with the application of the Design of Experiments (DoE) modulus from the STATISTICA Software.
28420283	1	20	theme	low-acyl	168:175	arg1	beads					190:194	low-acyl gellan macro beads	168:194	low-acyl gellan macro beads with the potential application as carriers for oral delivery of meloxicam (MLX) in the prophylaxis of colorectal cancer	168:314	The aim of the presented work was to design, formulate and evaluate the properties of low-acyl gellan macro beads with the potential application as carriers for oral delivery of meloxicam (MLX) in the prophylaxis of colorectal cancer.
28420283	10	21	theme	simulated	1174:1182	arg1	fluids					1201:1206	three simulated gastrointestinal fluids	1168:1206	three simulated gastrointestinal fluids at different pH (1.2; 4.5; 6.8)	1168:1238	The swelling behavior and degradation of the beads were evaluated in three simulated gastrointestinal fluids at different pH (1.2; 4.5; 6.8).
28420283	13	22	theme	gellan	1492:1497	arg1	gellan					1492:1497	gellan	1492:1497	gellan	1492:1497	The formulations obtained from mixtures containing 1.0 and 1.5% of gellan may be considered as oral dosage forms for MLX, intended to omit the stomach and release the drug in the distal parts of the gastrointestinal tract.
28420283	13	22	theme	gellan	1492:1497	arg1	%					1487:1487	1.0 and 1.5%	1476:1487	1.0 and 1.5% of gellan	1476:1497	The formulations obtained from mixtures containing 1.0 and 1.5% of gellan may be considered as oral dosage forms for MLX, intended to omit the stomach and release the drug in the distal parts of the gastrointestinal tract.
28420283	6	23	theme	encapsulation	798:810	arg1	efficiency					812:821	29.2-50.7% drug encapsulation efficiency	782:821	29.2-50.7% drug encapsulation efficiency	782:821	The prepared formulations revealed 7.2-27.0% of drug loading and 29.2-50.7% drug encapsulation efficiency.
28420283	12	24	theme	MLX	1374:1376	arg1	release					1378:1384	MLX release	1374:1384	MLX release from the dried beads	1374:1405	The obtained results showed that MLX release from the dried beads was pH-dependent.
28420283	9	25	theme	beads	1092:1096	arg1	production					1074:1083	production	1074:1083	production of the beads	1074:1096	Raman spectra confirmed that MLX did not undergo structural changes during production of the beads.
28420283	13	26	contain	containing	1465:1474	arg2	%					1487:1487	1.0 and 1.5%	1476:1487	1.0 and 1.5% of gellan	1476:1497	The formulations obtained from mixtures containing 1.0 and 1.5% of gellan may be considered as oral dosage forms for MLX, intended to omit the stomach and release the drug in the distal parts of the gastrointestinal tract.
28420283	13	26	contain	containing	1465:1474	arg2	gellan					1492:1497	gellan	1492:1497	gellan	1492:1497	The formulations obtained from mixtures containing 1.0 and 1.5% of gellan may be considered as oral dosage forms for MLX, intended to omit the stomach and release the drug in the distal parts of the gastrointestinal tract.
28420283	13	26	contain	containing	1465:1474	arg1	mixtures					1456:1463	mixtures	1456:1463	mixtures containing 1.0 and 1.5% of gellan	1456:1497	The formulations obtained from mixtures containing 1.0 and 1.5% of gellan may be considered as oral dosage forms for MLX, intended to omit the stomach and release the drug in the distal parts of the gastrointestinal tract.
28420283	11	27	theme	release	1258:1264	arg1	studies					1266:1272	The MLX in vitro release studies	1241:1272	The MLX in vitro release studies	1241:1272	The MLX in vitro release studies were conducted on USP apparatus IV, working in the open loop mode.
28420283	7	28	theme	gellan	858:863	arg1	gum					865:867	gellan gum	858:867	gellan gum	858:867	It turned out that 0.5% amount of gellan gum in the mixtures was not sufficient to obtain spherical beads.
28420283	4	29	theme	surfactant	492:501	arg1	mixtures					514:521	Nine different polymer, drug and surfactant (Tween®80) mixtures	459:521	Nine different polymer, drug and surfactant (Tween®80) mixtures	459:521	Nine different polymer, drug and surfactant (Tween®80) mixtures were used for production of the beads.
28420283	8	30	theme	dried	965:969	arg1	beads					971:975	the dried beads	961:975	the dried beads	961:975	The morphology and surface of the dried beads were analyzed by SEM.
28420283	7	31	theme	%	846:846	arg1	amount					848:853	0.5% amount	843:853	0.5% amount of gellan gum in the mixtures	843:883	It turned out that 0.5% amount of gellan gum in the mixtures was not sufficient to obtain spherical beads.
28420283	7	31	theme	%	846:846	arg1	gum					865:867	gellan gum	858:867	gellan gum	858:867	It turned out that 0.5% amount of gellan gum in the mixtures was not sufficient to obtain spherical beads.
28420283	13	32	theme	distal	1604:1609	arg1	parts					1611:1615	the distal parts	1600:1615	the distal parts of the gastrointestinal tract	1600:1645	The formulations obtained from mixtures containing 1.0 and 1.5% of gellan may be considered as oral dosage forms for MLX, intended to omit the stomach and release the drug in the distal parts of the gastrointestinal tract.
28420283	11	33	theme	USP	1292:1294	arg1	apparatus					1296:1304	USP apparatus IV	1292:1307	USP apparatus IV	1292:1307	The MLX in vitro release studies were conducted on USP apparatus IV, working in the open loop mode.
28420283	11	33	theme	USP	1292:1294	arg1	working					1310:1316	working	1310:1316	working	1310:1316	The MLX in vitro release studies were conducted on USP apparatus IV, working in the open loop mode.
28420283	11	34	dep	in	1249:1250	arg1	vitro					1252:1256	vitro	1252:1256	vitro	1252:1256	The MLX in vitro release studies were conducted on USP apparatus IV, working in the open loop mode.
28420283	1	35	theme	oral	243:246	arg1	delivery					248:255	oral delivery	243:255	oral delivery of meloxicam (MLX) in the prophylaxis of colorectal cancer	243:314	The aim of the presented work was to design, formulate and evaluate the properties of low-acyl gellan macro beads with the potential application as carriers for oral delivery of meloxicam (MLX) in the prophylaxis of colorectal cancer.
28420283	1	36	from	delivery	248:255	arg1	prophylaxis					283:293	the prophylaxis	279:293	the prophylaxis of colorectal cancer	279:314	The aim of the presented work was to design, formulate and evaluate the properties of low-acyl gellan macro beads with the potential application as carriers for oral delivery of meloxicam (MLX) in the prophylaxis of colorectal cancer.
28420283	7	37	theme	0.5	843:845	arg1	%					846:846	%	846:846	%	846:846	It turned out that 0.5% amount of gellan gum in the mixtures was not sufficient to obtain spherical beads.
28420283	13	38	theme	tract	1641:1645	arg1	parts					1611:1615	the distal parts	1600:1615	the distal parts of the gastrointestinal tract	1600:1645	The formulations obtained from mixtures containing 1.0 and 1.5% of gellan may be considered as oral dosage forms for MLX, intended to omit the stomach and release the drug in the distal parts of the gastrointestinal tract.
28420283	3	39	theme	Calcium	384:390	arg1	chloride					392:399	Calcium chloride	384:399	Calcium chloride (1.0%, 9.0 × 10-2 M)	384:420	Calcium chloride (1.0%, 9.0 × 10-2 M) was used as the cross-linking agent.
28420283	3	39	theme	Calcium	384:390	arg1	agent					452:456	the cross-linking agent	434:456	the cross-linking agent	434:456	Calcium chloride (1.0%, 9.0 × 10-2 M) was used as the cross-linking agent.
28420283	4	40	theme	drug	483:486	arg1	mixtures					514:521	Nine different polymer, drug and surfactant (Tween®80) mixtures	459:521	Nine different polymer, drug and surfactant (Tween®80) mixtures	459:521	Nine different polymer, drug and surfactant (Tween®80) mixtures were used for production of the beads.
28420283	7	41	theme	gum	865:867	arg1	amount					848:853	0.5% amount	843:853	0.5% amount of gellan gum in the mixtures	843:883	It turned out that 0.5% amount of gellan gum in the mixtures was not sufficient to obtain spherical beads.
28420283	7	41	theme	gum	865:867	arg1	gum					865:867	gellan gum	858:867	gellan gum	858:867	It turned out that 0.5% amount of gellan gum in the mixtures was not sufficient to obtain spherical beads.
28420283	10	42	theme	different	1211:1219	arg1	pH					1221:1222	different pH (1.2; 4.5; 6.8)	1211:1238	different pH (1.2; 4.5; 6.8)	1211:1238	The swelling behavior and degradation of the beads were evaluated in three simulated gastrointestinal fluids at different pH (1.2; 4.5; 6.8).
28420283	4	43	theme	beads	555:559	arg1	production					537:546	production	537:546	production of the beads	537:559	Nine different polymer, drug and surfactant (Tween®80) mixtures were used for production of the beads.
28420283	8	44	theme	beads	971:975	arg1	morphology					935:944	morphology	935:944	morphology	935:944	The morphology and surface of the dried beads were analyzed by SEM.
28420283	8	44	theme	beads	971:975	arg1	surface					950:956	surface	950:956	surface	950:956	The morphology and surface of the dried beads were analyzed by SEM.
28420283	6	45	theme	%	791:791	arg1	efficiency					812:821	29.2-50.7% drug encapsulation efficiency	782:821	29.2-50.7% drug encapsulation efficiency	782:821	The prepared formulations revealed 7.2-27.0% of drug loading and 29.2-50.7% drug encapsulation efficiency.
28420283	9	46	theme	Raman	999:1003	arg1	spectra					1005:1011	Raman spectra	999:1011	Raman spectra	999:1011	Raman spectra confirmed that MLX did not undergo structural changes during production of the beads.
28420283	2	47	theme	gelation	364:371	arg1	technique					373:381	ionotropic gelation technique	353:381	ionotropic gelation technique	353:381	The beads were obtained by means of ionotropic gelation technique.
28420283	0	48	theme	gum	37:39	arg1	beads					41:45	gellan gum beads	30:45	gellan gum beads	30:45	Design and characteristics of gellan gum beads for modified release of meloxicam.
28420283	1	49	theme	meloxicam	260:268	arg1	delivery					248:255	oral delivery	243:255	oral delivery of meloxicam (MLX) in the prophylaxis of colorectal cancer	243:314	The aim of the presented work was to design, formulate and evaluate the properties of low-acyl gellan macro beads with the potential application as carriers for oral delivery of meloxicam (MLX) in the prophylaxis of colorectal cancer.
28420283	4	50	used	used	528:531	arg2	mixtures					514:521	Nine different polymer, drug and surfactant (Tween®80) mixtures	459:521	Nine different polymer, drug and surfactant (Tween®80) mixtures	459:521	Nine different polymer, drug and surfactant (Tween®80) mixtures were used for production of the beads.
28420283	1	51	theme	gellan	177:182	arg1	beads					190:194	low-acyl gellan macro beads	168:194	low-acyl gellan macro beads with the potential application as carriers for oral delivery of meloxicam (MLX) in the prophylaxis of colorectal cancer	168:314	The aim of the presented work was to design, formulate and evaluate the properties of low-acyl gellan macro beads with the potential application as carriers for oral delivery of meloxicam (MLX) in the prophylaxis of colorectal cancer.
28420283	2	52	theme	ionotropic	353:362	arg1	technique					373:381	ionotropic gelation technique	353:381	ionotropic gelation technique	353:381	The beads were obtained by means of ionotropic gelation technique.
28420283	0	53	theme	gellan	30:35	arg1	beads					41:45	gellan gum beads	30:45	gellan gum beads	30:45	Design and characteristics of gellan gum beads for modified release of meloxicam.
28420283	4	54	theme	different	464:472	arg1	mixtures					514:521	Nine different polymer, drug and surfactant (Tween®80) mixtures	459:521	Nine different polymer, drug and surfactant (Tween®80) mixtures	459:521	Nine different polymer, drug and surfactant (Tween®80) mixtures were used for production of the beads.
28420283	5	55	theme	STATISTICA	696:705	arg1	Software					707:714	the STATISTICA Software	692:714	the STATISTICA Software	692:714	The quantitative compositions of the mixtures were generated with the application of the Design of Experiments (DoE) modulus from the STATISTICA Software.
28420283	5	56	from	Design	651:656	arg1	Software					707:714	the STATISTICA Software	692:714	the STATISTICA Software	692:714	The quantitative compositions of the mixtures were generated with the application of the Design of Experiments (DoE) modulus from the STATISTICA Software.
28420283	7	57	theme	spherical	914:922	arg1	beads					924:928	spherical beads	914:928	spherical beads	914:928	It turned out that 0.5% amount of gellan gum in the mixtures was not sufficient to obtain spherical beads.
28420283	1	58	theme	macro	184:188	arg1	beads					190:194	low-acyl gellan macro beads	168:194	low-acyl gellan macro beads with the potential application as carriers for oral delivery of meloxicam (MLX) in the prophylaxis of colorectal cancer	168:314	The aim of the presented work was to design, formulate and evaluate the properties of low-acyl gellan macro beads with the potential application as carriers for oral delivery of meloxicam (MLX) in the prophylaxis of colorectal cancer.
28420283	5	59	theme	mixtures	599:606	arg1	compositions					579:590	The quantitative compositions	562:590	The quantitative compositions of the mixtures	562:606	The quantitative compositions of the mixtures were generated with the application of the Design of Experiments (DoE) modulus from the STATISTICA Software.
28420283	6	60	theme	loading	770:776	arg1	efficiency					812:821	29.2-50.7% drug encapsulation efficiency	782:821	29.2-50.7% drug encapsulation efficiency	782:821	The prepared formulations revealed 7.2-27.0% of drug loading and 29.2-50.7% drug encapsulation efficiency.
28420283	6	60	theme	loading	770:776	arg1	%					760:760	7.2-27.0%	752:760	7.2-27.0% of drug loading	752:776	The prepared formulations revealed 7.2-27.0% of drug loading and 29.2-50.7% drug encapsulation efficiency.
28420283	6	60	theme	loading	770:776	arg1	loading					770:776	drug loading	765:776	drug loading	765:776	The prepared formulations revealed 7.2-27.0% of drug loading and 29.2-50.7% drug encapsulation efficiency.
28420283	3	61	dep	chloride	392:399	arg1	%					405:405	1.0%	402:405	1.0%	402:405	Calcium chloride (1.0%, 9.0 × 10-2 M) was used as the cross-linking agent.
28420283	3	61	dep	chloride	392:399	arg1	9.0 × 10-2 M					408:419	9.0 × 10-2 M	408:419	9.0 × 10-2 M	408:419	Calcium chloride (1.0%, 9.0 × 10-2 M) was used as the cross-linking agent.
28420283	0	62	theme	beads	41:45	arg1	characteristics					11:25	characteristics	11:25	characteristics	11:25	Design and characteristics of gellan gum beads for modified release of meloxicam.
28420283	0	62	theme	beads	41:45	arg1	Design					0:5	Design	0:5	Design	0:5	Design and characteristics of gellan gum beads for modified release of meloxicam.
28420283	6	63	theme	drug	765:768	arg1	loading					770:776	drug loading	765:776	drug loading	765:776	The prepared formulations revealed 7.2-27.0% of drug loading and 29.2-50.7% drug encapsulation efficiency.
28420283	3	64	used	used	426:429	arg2	agent					452:456	the cross-linking agent	434:456	the cross-linking agent	434:456	Calcium chloride (1.0%, 9.0 × 10-2 M) was used as the cross-linking agent.
28420283	3	64	used	used	426:429	arg2	chloride					392:399	Calcium chloride	384:399	Calcium chloride (1.0%, 9.0 × 10-2 M)	384:420	Calcium chloride (1.0%, 9.0 × 10-2 M) was used as the cross-linking agent.
28420283	1	65	theme	presented	97:105	arg1	work					107:110	the presented work	93:110	the presented work	93:110	The aim of the presented work was to design, formulate and evaluate the properties of low-acyl gellan macro beads with the potential application as carriers for oral delivery of meloxicam (MLX) in the prophylaxis of colorectal cancer.
28420283	4	66	theme	polymer	474:480	arg1	mixtures					514:521	Nine different polymer, drug and surfactant (Tween®80) mixtures	459:521	Nine different polymer, drug and surfactant (Tween®80) mixtures	459:521	Nine different polymer, drug and surfactant (Tween®80) mixtures were used for production of the beads.
28420283	7	67	dep	turned	827:832	arg1	out					834:836	out	834:836	out	834:836	It turned out that 0.5% amount of gellan gum in the mixtures was not sufficient to obtain spherical beads.
28420283	1	68	theme	work	107:110	arg1	aim					86:88	The aim	82:88	The aim of the presented work	82:110	The aim of the presented work was to design, formulate and evaluate the properties of low-acyl gellan macro beads with the potential application as carriers for oral delivery of meloxicam (MLX) in the prophylaxis of colorectal cancer.
28420283	9	69	theme	structural	1048:1057	arg1	changes					1059:1065	structural changes	1048:1065	structural changes	1048:1065	Raman spectra confirmed that MLX did not undergo structural changes during production of the beads.
28420283	1	70	theme	beads	190:194	arg1	properties					154:163	the properties	150:163	the properties of low-acyl gellan macro beads with the potential application as carriers for oral delivery of meloxicam (MLX) in the prophylaxis of colorectal cancer	150:314	The aim of the presented work was to design, formulate and evaluate the properties of low-acyl gellan macro beads with the potential application as carriers for oral delivery of meloxicam (MLX) in the prophylaxis of colorectal cancer.
28420283	13	71	theme	dosage	1525:1530	arg1	formulations					1429:1440	The formulations	1425:1440	The formulations obtained from mixtures containing 1.0 and 1.5% of gellan	1425:1497	The formulations obtained from mixtures containing 1.0 and 1.5% of gellan may be considered as oral dosage forms for MLX, intended to omit the stomach and release the drug in the distal parts of the gastrointestinal tract.
28420283	13	71	theme	dosage	1525:1530	arg1	forms					1532:1536	oral dosage forms	1520:1536	oral dosage forms	1520:1536	The formulations obtained from mixtures containing 1.0 and 1.5% of gellan may be considered as oral dosage forms for MLX, intended to omit the stomach and release the drug in the distal parts of the gastrointestinal tract.
28420283	11	72	theme	open	1325:1328	arg1	mode					1335:1338	the open loop mode	1321:1338	the open loop mode	1321:1338	The MLX in vitro release studies were conducted on USP apparatus IV, working in the open loop mode.
28420283	7	73	from	amount	848:853	arg1	mixtures					876:883	the mixtures	872:883	the mixtures	872:883	It turned out that 0.5% amount of gellan gum in the mixtures was not sufficient to obtain spherical beads.
28420283	5	74	theme	DoE	674:676	arg1	modulus					679:685	Experiments (DoE) modulus	661:685	Experiments (DoE) modulus from the STATISTICA Software	661:714	The quantitative compositions of the mixtures were generated with the application of the Design of Experiments (DoE) modulus from the STATISTICA Software.
28420283	13	75	theme	oral	1520:1523	arg1	formulations					1429:1440	The formulations	1425:1440	The formulations obtained from mixtures containing 1.0 and 1.5% of gellan	1425:1497	The formulations obtained from mixtures containing 1.0 and 1.5% of gellan may be considered as oral dosage forms for MLX, intended to omit the stomach and release the drug in the distal parts of the gastrointestinal tract.
28420283	13	75	theme	oral	1520:1523	arg1	forms					1532:1536	oral dosage forms	1520:1536	oral dosage forms	1520:1536	The formulations obtained from mixtures containing 1.0 and 1.5% of gellan may be considered as oral dosage forms for MLX, intended to omit the stomach and release the drug in the distal parts of the gastrointestinal tract.
28420283	10	76	from	pH	1221:1222	arg1	fluids					1201:1206	three simulated gastrointestinal fluids	1168:1206	three simulated gastrointestinal fluids at different pH (1.2; 4.5; 6.8)	1168:1238	The swelling behavior and degradation of the beads were evaluated in three simulated gastrointestinal fluids at different pH (1.2; 4.5; 6.8).
28420283	11	77	theme	loop	1330:1333	arg1	mode					1335:1338	the open loop mode	1321:1338	the open loop mode	1321:1338	The MLX in vitro release studies were conducted on USP apparatus IV, working in the open loop mode.
28420283	13	78	from	drug	1592:1595	arg1	parts					1611:1615	the distal parts	1600:1615	the distal parts of the gastrointestinal tract	1600:1645	The formulations obtained from mixtures containing 1.0 and 1.5% of gellan may be considered as oral dosage forms for MLX, intended to omit the stomach and release the drug in the distal parts of the gastrointestinal tract.
28420283	0	79	theme	modified	51:58	arg1	release					60:66	modified release	51:66	modified release of meloxicam	51:79	Design and characteristics of gellan gum beads for modified release of meloxicam.
28420283	5	80	from	Software	707:714	arg1	Design					651:656	the Design	647:656	the Design of Experiments (DoE) modulus from the STATISTICA Software	647:714	The quantitative compositions of the mixtures were generated with the application of the Design of Experiments (DoE) modulus from the STATISTICA Software.
28420283	5	80	from	Software	707:714	arg1	modulus					679:685	Experiments (DoE) modulus	661:685	Experiments (DoE) modulus from the STATISTICA Software	661:714	The quantitative compositions of the mixtures were generated with the application of the Design of Experiments (DoE) modulus from the STATISTICA Software.
28420283	1	81	with	beads	190:194	arg1	application					215:225	the potential application	201:225	the potential application as carriers for oral delivery of meloxicam (MLX) in the prophylaxis of colorectal cancer	201:314	The aim of the presented work was to design, formulate and evaluate the properties of low-acyl gellan macro beads with the potential application as carriers for oral delivery of meloxicam (MLX) in the prophylaxis of colorectal cancer.
28420283	12	82	theme	dried	1395:1399	arg1	beads					1401:1405	the dried beads	1391:1405	the dried beads	1391:1405	The obtained results showed that MLX release from the dried beads was pH-dependent.
28420283	1	83	theme	potential	205:213	arg1	application					215:225	the potential application	201:225	the potential application as carriers for oral delivery of meloxicam (MLX) in the prophylaxis of colorectal cancer	201:314	The aim of the presented work was to design, formulate and evaluate the properties of low-acyl gellan macro beads with the potential application as carriers for oral delivery of meloxicam (MLX) in the prophylaxis of colorectal cancer.
26680323	7	0	theme	complex	1075:1081	arg1	phosphoproteomes					1083:1098	complex phosphoproteomes	1075:1098	complex phosphoproteomes	1075:1098	Hence, this method had great potential for future studies of complex phosphoproteomes and especially multi-phosphorylated peptides.
26680323	6	1	theme	S	968:968	arg1	*					973:973	S*S*S*	968:973	S*S*S*	968:973	Furthermore, multi-phosphorylated peptides with three serine phospholated sites (S*S*S*) demonstrated high affinity to Ti-CTS.
26680323	6	1	theme	S	968:968	arg1	sites					961:965	three serine phospholated sites	935:965	three serine phospholated sites (S*S*S*)	935:974	Furthermore, multi-phosphorylated peptides with three serine phospholated sites (S*S*S*) demonstrated high affinity to Ti-CTS.
26680323	4	2	theme	non-fat	636:642	arg1	samples					627:633	two real samples	618:633	two real samples (non-fat milk and serum)	618:658	Tryptic digest products from several standard proteins and two real samples (non-fat milk and serum) were enriched using Ti-CTS to demonstrate the efficiency of this method.
26680323	4	2	theme	non-fat	636:642	arg1	milk					644:647	non-fat milk	636:647	non-fat milk	636:647	Tryptic digest products from several standard proteins and two real samples (non-fat milk and serum) were enriched using Ti-CTS to demonstrate the efficiency of this method.
26680323	3	3	theme	electron	452:459	arg1	microscopy					461:470	scanning electron microscopy	443:470	scanning electron microscopy (SEM)	443:476	The morphology, surface area, pore volume and elemental composition of Ti-CTS were revealed by scanning electron microscopy (SEM), Brunauer-Emmett-Teller (BET) method and X-ray photoelectron spectroscopy (XPS).
26680323	3	3	theme	electron	452:459	arg1	SEM					473:475	SEM	473:475	SEM	473:475	The morphology, surface area, pore volume and elemental composition of Ti-CTS were revealed by scanning electron microscopy (SEM), Brunauer-Emmett-Teller (BET) method and X-ray photoelectron spectroscopy (XPS).
26680323	0	4	theme	chitosan	71:78	arg1	composite					80:88	titanium(IV) cross-linked chitosan composite	45:88	titanium(IV) cross-linked chitosan composite	45:88	Highly specific phosphopeptide enrichment by titanium(IV) cross-linked chitosan composite.
26680323	7	5	theme	great	1037:1041	arg1	potential					1043:1051	great potential	1037:1051	great potential for future studies of complex phosphoproteomes and especially multi-phosphorylated peptides	1037:1143	Hence, this method had great potential for future studies of complex phosphoproteomes and especially multi-phosphorylated peptides.
26680323	5	6	from	casein	840:845	arg1	enrichment					824:833	high sensitive and selective phosphopeptide enrichment	780:833	high sensitive and selective phosphopeptide enrichment from casein variants, non-fat milk and human serum	780:884	The results showed that this composite enables high sensitive and selective phosphopeptide enrichment from casein variants, non-fat milk and human serum.
26680323	6	7	theme	phospholated	948:959	arg1	*					973:973	S*S*S*	968:973	S*S*S*	968:973	Furthermore, multi-phosphorylated peptides with three serine phospholated sites (S*S*S*) demonstrated high affinity to Ti-CTS.
26680323	6	7	theme	phospholated	948:959	arg1	sites					961:965	three serine phospholated sites	935:965	three serine phospholated sites (S*S*S*)	935:974	Furthermore, multi-phosphorylated peptides with three serine phospholated sites (S*S*S*) demonstrated high affinity to Ti-CTS.
26680323	7	8	theme	peptides	1136:1143	arg1	studies					1064:1070	future studies	1057:1070	future studies of complex phosphoproteomes and especially multi-phosphorylated peptides	1057:1143	Hence, this method had great potential for future studies of complex phosphoproteomes and especially multi-phosphorylated peptides.
26680323	2	9	theme	based	261:265	arg1	IMAC					267:270	other polymer based IMAC	247:270	other polymer based IMAC	247:270	Compared with other polymer based IMAC, Ti-CTS can save the cockamamie synthesis procedures and be easy to obtain.
26680323	6	10	theme	S	972:972	arg1	*					973:973	S*S*S*	968:973	S*S*S*	968:973	Furthermore, multi-phosphorylated peptides with three serine phospholated sites (S*S*S*) demonstrated high affinity to Ti-CTS.
26680323	6	10	theme	S	972:972	arg1	sites					961:965	three serine phospholated sites	935:965	three serine phospholated sites (S*S*S*)	935:974	Furthermore, multi-phosphorylated peptides with three serine phospholated sites (S*S*S*) demonstrated high affinity to Ti-CTS.
26680323	6	11	theme	serine	941:946	arg1	*					973:973	S*S*S*	968:973	S*S*S*	968:973	Furthermore, multi-phosphorylated peptides with three serine phospholated sites (S*S*S*) demonstrated high affinity to Ti-CTS.
26680323	6	11	theme	serine	941:946	arg1	sites					961:965	three serine phospholated sites	935:965	three serine phospholated sites (S*S*S*)	935:974	Furthermore, multi-phosphorylated peptides with three serine phospholated sites (S*S*S*) demonstrated high affinity to Ti-CTS.
26680323	5	12	dep	casein	840:845	arg1	variants					847:854	variants	847:854	variants	847:854	The results showed that this composite enables high sensitive and selective phosphopeptide enrichment from casein variants, non-fat milk and human serum.
26680323	4	13	theme	digest	567:572	arg1	products					574:581	Tryptic digest products	559:581	Tryptic digest products from several standard proteins	559:612	Tryptic digest products from several standard proteins and two real samples (non-fat milk and serum) were enriched using Ti-CTS to demonstrate the efficiency of this method.
26680323	7	14	contain	had	1033:1035	arg1	method					1026:1031	this method	1021:1031	this method	1021:1031	Hence, this method had great potential for future studies of complex phosphoproteomes and especially multi-phosphorylated peptides.
26680323	7	14	contain	had	1033:1035	arg2	potential					1043:1051	great potential	1037:1051	great potential for future studies of complex phosphoproteomes and especially multi-phosphorylated peptides	1037:1143	Hence, this method had great potential for future studies of complex phosphoproteomes and especially multi-phosphorylated peptides.
26680323	2	15	theme	polymer	253:259	arg1	IMAC					267:270	other polymer based IMAC	247:270	other polymer based IMAC	247:270	Compared with other polymer based IMAC, Ti-CTS can save the cockamamie synthesis procedures and be easy to obtain.
26680323	4	16	theme	several	588:594	arg1	proteins					605:612	several standard proteins	588:612	several standard proteins	588:612	Tryptic digest products from several standard proteins and two real samples (non-fat milk and serum) were enriched using Ti-CTS to demonstrate the efficiency of this method.
26680323	3	17	dep	morphology	352:361	arg1	The					348:350	The	348:350	The	348:350	The morphology, surface area, pore volume and elemental composition of Ti-CTS were revealed by scanning electron microscopy (SEM), Brunauer-Emmett-Teller (BET) method and X-ray photoelectron spectroscopy (XPS).
26680323	3	18	theme	Ti-CTS	419:424	arg1	volume					383:388	pore volume	378:388	pore volume	378:388	The morphology, surface area, pore volume and elemental composition of Ti-CTS were revealed by scanning electron microscopy (SEM), Brunauer-Emmett-Teller (BET) method and X-ray photoelectron spectroscopy (XPS).
26680323	3	18	theme	Ti-CTS	419:424	arg1	composition					404:414	elemental composition	394:414	elemental composition	394:414	The morphology, surface area, pore volume and elemental composition of Ti-CTS were revealed by scanning electron microscopy (SEM), Brunauer-Emmett-Teller (BET) method and X-ray photoelectron spectroscopy (XPS).
26680323	3	18	theme	Ti-CTS	419:424	arg1	morphology					352:361	morphology	352:361	morphology	352:361	The morphology, surface area, pore volume and elemental composition of Ti-CTS were revealed by scanning electron microscopy (SEM), Brunauer-Emmett-Teller (BET) method and X-ray photoelectron spectroscopy (XPS).
26680323	3	18	theme	Ti-CTS	419:424	arg1	area					372:375	surface area	364:375	surface area	364:375	The morphology, surface area, pore volume and elemental composition of Ti-CTS were revealed by scanning electron microscopy (SEM), Brunauer-Emmett-Teller (BET) method and X-ray photoelectron spectroscopy (XPS).
26680323	2	19	theme	other	247:251	arg1	IMAC					267:270	other polymer based IMAC	247:270	other polymer based IMAC	247:270	Compared with other polymer based IMAC, Ti-CTS can save the cockamamie synthesis procedures and be easy to obtain.
26680323	5	20	theme	selective	799:807	arg1	enrichment					824:833	high sensitive and selective phosphopeptide enrichment	780:833	high sensitive and selective phosphopeptide enrichment from casein variants, non-fat milk and human serum	780:884	The results showed that this composite enables high sensitive and selective phosphopeptide enrichment from casein variants, non-fat milk and human serum.
26680323	5	21	theme	sensitive	785:793	arg1	enrichment					824:833	high sensitive and selective phosphopeptide enrichment	780:833	high sensitive and selective phosphopeptide enrichment from casein variants, non-fat milk and human serum	780:884	The results showed that this composite enables high sensitive and selective phosphopeptide enrichment from casein variants, non-fat milk and human serum.
26680323	7	22	theme	multi-phosphorylated	1115:1134	arg1	peptides					1136:1143	especially multi-phosphorylated peptides	1104:1143	especially multi-phosphorylated peptides	1104:1143	Hence, this method had great potential for future studies of complex phosphoproteomes and especially multi-phosphorylated peptides.
26680323	7	23	theme	future	1057:1062	arg1	studies					1064:1070	future studies	1057:1070	future studies of complex phosphoproteomes and especially multi-phosphorylated peptides	1057:1143	Hence, this method had great potential for future studies of complex phosphoproteomes and especially multi-phosphorylated peptides.
26680323	5	24	from	milk	865:868	arg1	enrichment					824:833	high sensitive and selective phosphopeptide enrichment	780:833	high sensitive and selective phosphopeptide enrichment from casein variants, non-fat milk and human serum	780:884	The results showed that this composite enables high sensitive and selective phosphopeptide enrichment from casein variants, non-fat milk and human serum.
26680323	6	25	with	peptides	921:928	arg1	*					973:973	S*S*S*	968:973	S*S*S*	968:973	Furthermore, multi-phosphorylated peptides with three serine phospholated sites (S*S*S*) demonstrated high affinity to Ti-CTS.
26680323	6	25	with	peptides	921:928	arg1	sites					961:965	three serine phospholated sites	935:965	three serine phospholated sites (S*S*S*)	935:974	Furthermore, multi-phosphorylated peptides with three serine phospholated sites (S*S*S*) demonstrated high affinity to Ti-CTS.
26680323	4	26	from	proteins	605:612	arg1	serum					653:657	serum	653:657	serum	653:657	Tryptic digest products from several standard proteins and two real samples (non-fat milk and serum) were enriched using Ti-CTS to demonstrate the efficiency of this method.
26680323	4	26	from	proteins	605:612	arg1	products					574:581	Tryptic digest products	559:581	Tryptic digest products from several standard proteins	559:612	Tryptic digest products from several standard proteins and two real samples (non-fat milk and serum) were enriched using Ti-CTS to demonstrate the efficiency of this method.
26680323	4	26	from	proteins	605:612	arg1	milk					644:647	non-fat milk	636:647	non-fat milk	636:647	Tryptic digest products from several standard proteins and two real samples (non-fat milk and serum) were enriched using Ti-CTS to demonstrate the efficiency of this method.
26680323	4	26	from	proteins	605:612	arg1	samples					627:633	two real samples	618:633	two real samples (non-fat milk and serum)	618:658	Tryptic digest products from several standard proteins and two real samples (non-fat milk and serum) were enriched using Ti-CTS to demonstrate the efficiency of this method.
26680323	1	27	theme	based	154:158	arg1	Ti-CTS					224:229	Ti(IV) based immobilized metal ion affinity chromatographic (IMAC) material (Ti-CTS)	147:230	Ti(IV) based immobilized metal ion affinity chromatographic (IMAC) material (Ti-CTS)	147:230	Natural chitosan was applied as supporting material for Ti(IV) based immobilized metal ion affinity chromatographic (IMAC) material (Ti-CTS).
26680323	0	28	theme	specific	7:14	arg1	enrichment					31:40	Highly specific phosphopeptide enrichment	0:40	Highly specific phosphopeptide enrichment	0:40	Highly specific phosphopeptide enrichment by titanium(IV) cross-linked chitosan composite.
26680323	5	29	theme	human	874:878	arg1	serum					880:884	human serum	874:884	human serum	874:884	The results showed that this composite enables high sensitive and selective phosphopeptide enrichment from casein variants, non-fat milk and human serum.
26680323	6	30	theme	high	989:992	arg1	affinity					994:1001	high affinity	989:1001	high affinity to Ti-CTS	989:1011	Furthermore, multi-phosphorylated peptides with three serine phospholated sites (S*S*S*) demonstrated high affinity to Ti-CTS.
26680323	1	31	theme	immobilized	160:170	arg1	Ti-CTS					224:229	Ti(IV) based immobilized metal ion affinity chromatographic (IMAC) material (Ti-CTS)	147:230	Ti(IV) based immobilized metal ion affinity chromatographic (IMAC) material (Ti-CTS)	147:230	Natural chitosan was applied as supporting material for Ti(IV) based immobilized metal ion affinity chromatographic (IMAC) material (Ti-CTS).
26680323	1	32	theme	Natural	91:97	arg1	chitosan					99:106	Natural chitosan	91:106	Natural chitosan	91:106	Natural chitosan was applied as supporting material for Ti(IV) based immobilized metal ion affinity chromatographic (IMAC) material (Ti-CTS).
26680323	1	33	theme	metal	172:176	arg1	Ti-CTS					224:229	Ti(IV) based immobilized metal ion affinity chromatographic (IMAC) material (Ti-CTS)	147:230	Ti(IV) based immobilized metal ion affinity chromatographic (IMAC) material (Ti-CTS)	147:230	Natural chitosan was applied as supporting material for Ti(IV) based immobilized metal ion affinity chromatographic (IMAC) material (Ti-CTS).
26680323	3	34	theme	BET	503:505	arg1	method					508:513	Brunauer-Emmett-Teller (BET) method	479:513	Brunauer-Emmett-Teller (BET) method	479:513	The morphology, surface area, pore volume and elemental composition of Ti-CTS were revealed by scanning electron microscopy (SEM), Brunauer-Emmett-Teller (BET) method and X-ray photoelectron spectroscopy (XPS).
26680323	1	35	theme	ion	178:180	arg1	Ti-CTS					224:229	Ti(IV) based immobilized metal ion affinity chromatographic (IMAC) material (Ti-CTS)	147:230	Ti(IV) based immobilized metal ion affinity chromatographic (IMAC) material (Ti-CTS)	147:230	Natural chitosan was applied as supporting material for Ti(IV) based immobilized metal ion affinity chromatographic (IMAC) material (Ti-CTS).
26680323	0	36	theme	phosphopeptide	16:29	arg1	enrichment					31:40	Highly specific phosphopeptide enrichment	0:40	Highly specific phosphopeptide enrichment	0:40	Highly specific phosphopeptide enrichment by titanium(IV) cross-linked chitosan composite.
26680323	6	37	theme	multi-phosphorylated	900:919	arg1	peptides					921:928	multi-phosphorylated peptides	900:928	multi-phosphorylated peptides with three serine phospholated sites (S*S*S*)	900:974	Furthermore, multi-phosphorylated peptides with three serine phospholated sites (S*S*S*) demonstrated high affinity to Ti-CTS.
26680323	4	38	dep	samples	627:633	arg1	serum					653:657	serum	653:657	serum	653:657	Tryptic digest products from several standard proteins and two real samples (non-fat milk and serum) were enriched using Ti-CTS to demonstrate the efficiency of this method.
26680323	4	38	dep	samples	627:633	arg1	samples					627:633	two real samples	618:633	two real samples (non-fat milk and serum)	618:658	Tryptic digest products from several standard proteins and two real samples (non-fat milk and serum) were enriched using Ti-CTS to demonstrate the efficiency of this method.
26680323	4	38	dep	samples	627:633	arg1	milk					644:647	non-fat milk	636:647	non-fat milk	636:647	Tryptic digest products from several standard proteins and two real samples (non-fat milk and serum) were enriched using Ti-CTS to demonstrate the efficiency of this method.
26680323	1	39	theme	affinity	182:189	arg1	Ti-CTS					224:229	Ti(IV) based immobilized metal ion affinity chromatographic (IMAC) material (Ti-CTS)	147:230	Ti(IV) based immobilized metal ion affinity chromatographic (IMAC) material (Ti-CTS)	147:230	Natural chitosan was applied as supporting material for Ti(IV) based immobilized metal ion affinity chromatographic (IMAC) material (Ti-CTS).
26680323	3	40	theme	surface	364:370	arg1	area					372:375	surface area	364:375	surface area	364:375	The morphology, surface area, pore volume and elemental composition of Ti-CTS were revealed by scanning electron microscopy (SEM), Brunauer-Emmett-Teller (BET) method and X-ray photoelectron spectroscopy (XPS).
26680323	3	41	theme	scanning	443:450	arg1	microscopy					461:470	scanning electron microscopy	443:470	scanning electron microscopy (SEM)	443:476	The morphology, surface area, pore volume and elemental composition of Ti-CTS were revealed by scanning electron microscopy (SEM), Brunauer-Emmett-Teller (BET) method and X-ray photoelectron spectroscopy (XPS).
26680323	3	41	theme	scanning	443:450	arg1	SEM					473:475	SEM	473:475	SEM	473:475	The morphology, surface area, pore volume and elemental composition of Ti-CTS were revealed by scanning electron microscopy (SEM), Brunauer-Emmett-Teller (BET) method and X-ray photoelectron spectroscopy (XPS).
26680323	3	42	theme	elemental	394:402	arg1	composition					404:414	elemental composition	394:414	elemental composition	394:414	The morphology, surface area, pore volume and elemental composition of Ti-CTS were revealed by scanning electron microscopy (SEM), Brunauer-Emmett-Teller (BET) method and X-ray photoelectron spectroscopy (XPS).
26680323	3	43	theme	pore	378:381	arg1	volume					383:388	pore volume	378:388	pore volume	378:388	The morphology, surface area, pore volume and elemental composition of Ti-CTS were revealed by scanning electron microscopy (SEM), Brunauer-Emmett-Teller (BET) method and X-ray photoelectron spectroscopy (XPS).
26680323	1	44	theme	chromatographic	191:205	arg1	Ti-CTS					224:229	Ti(IV) based immobilized metal ion affinity chromatographic (IMAC) material (Ti-CTS)	147:230	Ti(IV) based immobilized metal ion affinity chromatographic (IMAC) material (Ti-CTS)	147:230	Natural chitosan was applied as supporting material for Ti(IV) based immobilized metal ion affinity chromatographic (IMAC) material (Ti-CTS).
26680323	6	45	theme	S	970:970	arg1	*					973:973	S*S*S*	968:973	S*S*S*	968:973	Furthermore, multi-phosphorylated peptides with three serine phospholated sites (S*S*S*) demonstrated high affinity to Ti-CTS.
26680323	6	45	theme	S	970:970	arg1	sites					961:965	three serine phospholated sites	935:965	three serine phospholated sites (S*S*S*)	935:974	Furthermore, multi-phosphorylated peptides with three serine phospholated sites (S*S*S*) demonstrated high affinity to Ti-CTS.
26680323	4	46	theme	Tryptic	559:565	arg1	products					574:581	Tryptic digest products	559:581	Tryptic digest products from several standard proteins	559:612	Tryptic digest products from several standard proteins and two real samples (non-fat milk and serum) were enriched using Ti-CTS to demonstrate the efficiency of this method.
26680323	5	47	theme	high	780:783	arg1	enrichment					824:833	high sensitive and selective phosphopeptide enrichment	780:833	high sensitive and selective phosphopeptide enrichment from casein variants, non-fat milk and human serum	780:884	The results showed that this composite enables high sensitive and selective phosphopeptide enrichment from casein variants, non-fat milk and human serum.
26680323	3	48	theme	X-ray	519:523	arg1	spectroscopy					539:550	X-ray photoelectron spectroscopy	519:550	X-ray photoelectron spectroscopy (XPS)	519:556	The morphology, surface area, pore volume and elemental composition of Ti-CTS were revealed by scanning electron microscopy (SEM), Brunauer-Emmett-Teller (BET) method and X-ray photoelectron spectroscopy (XPS).
26680323	3	48	theme	X-ray	519:523	arg1	XPS					553:555	XPS	553:555	XPS	553:555	The morphology, surface area, pore volume and elemental composition of Ti-CTS were revealed by scanning electron microscopy (SEM), Brunauer-Emmett-Teller (BET) method and X-ray photoelectron spectroscopy (XPS).
26680323	2	49	theme	synthesis	304:312	arg1	procedures					314:323	the cockamamie synthesis procedures	289:323	the cockamamie synthesis procedures	289:323	Compared with other polymer based IMAC, Ti-CTS can save the cockamamie synthesis procedures and be easy to obtain.
26680323	3	50	theme	photoelectron	525:537	arg1	spectroscopy					539:550	X-ray photoelectron spectroscopy	519:550	X-ray photoelectron spectroscopy (XPS)	519:556	The morphology, surface area, pore volume and elemental composition of Ti-CTS were revealed by scanning electron microscopy (SEM), Brunauer-Emmett-Teller (BET) method and X-ray photoelectron spectroscopy (XPS).
26680323	3	50	theme	photoelectron	525:537	arg1	XPS					553:555	XPS	553:555	XPS	553:555	The morphology, surface area, pore volume and elemental composition of Ti-CTS were revealed by scanning electron microscopy (SEM), Brunauer-Emmett-Teller (BET) method and X-ray photoelectron spectroscopy (XPS).
26680323	0	51	link	cross-linked	58:69	arg1	composite					80:88	titanium(IV) cross-linked chitosan composite	45:88	titanium(IV) cross-linked chitosan composite	45:88	Highly specific phosphopeptide enrichment by titanium(IV) cross-linked chitosan composite.
26680323	2	52	theme	cockamamie	293:302	arg1	procedures					314:323	the cockamamie synthesis procedures	289:323	the cockamamie synthesis procedures	289:323	Compared with other polymer based IMAC, Ti-CTS can save the cockamamie synthesis procedures and be easy to obtain.
26680323	5	53	from	serum	880:884	arg1	enrichment					824:833	high sensitive and selective phosphopeptide enrichment	780:833	high sensitive and selective phosphopeptide enrichment from casein variants, non-fat milk and human serum	780:884	The results showed that this composite enables high sensitive and selective phosphopeptide enrichment from casein variants, non-fat milk and human serum.
26680323	5	54	theme	non-fat	857:863	arg1	milk					865:868	non-fat milk	857:868	non-fat milk	857:868	The results showed that this composite enables high sensitive and selective phosphopeptide enrichment from casein variants, non-fat milk and human serum.
26680323	0	55	dep	enrichment	31:40	arg1	composite					80:88	titanium(IV) cross-linked chitosan composite	45:88	titanium(IV) cross-linked chitosan composite	45:88	Highly specific phosphopeptide enrichment by titanium(IV) cross-linked chitosan composite.
26680323	5	56	theme	phosphopeptide	809:822	arg1	enrichment					824:833	high sensitive and selective phosphopeptide enrichment	780:833	high sensitive and selective phosphopeptide enrichment from casein variants, non-fat milk and human serum	780:884	The results showed that this composite enables high sensitive and selective phosphopeptide enrichment from casein variants, non-fat milk and human serum.
26680323	1	57	theme	IMAC	208:211	arg1	Ti-CTS					224:229	Ti(IV) based immobilized metal ion affinity chromatographic (IMAC) material (Ti-CTS)	147:230	Ti(IV) based immobilized metal ion affinity chromatographic (IMAC) material (Ti-CTS)	147:230	Natural chitosan was applied as supporting material for Ti(IV) based immobilized metal ion affinity chromatographic (IMAC) material (Ti-CTS).
26680323	3	58	theme	Brunauer-Emmett-Teller	479:500	arg1	method					508:513	Brunauer-Emmett-Teller (BET) method	479:513	Brunauer-Emmett-Teller (BET) method	479:513	The morphology, surface area, pore volume and elemental composition of Ti-CTS were revealed by scanning electron microscopy (SEM), Brunauer-Emmett-Teller (BET) method and X-ray photoelectron spectroscopy (XPS).
26680323	4	59	theme	real	622:625	arg1	serum					653:657	serum	653:657	serum	653:657	Tryptic digest products from several standard proteins and two real samples (non-fat milk and serum) were enriched using Ti-CTS to demonstrate the efficiency of this method.
26680323	4	59	theme	real	622:625	arg1	samples					627:633	two real samples	618:633	two real samples (non-fat milk and serum)	618:658	Tryptic digest products from several standard proteins and two real samples (non-fat milk and serum) were enriched using Ti-CTS to demonstrate the efficiency of this method.
26680323	4	59	theme	real	622:625	arg1	milk					644:647	non-fat milk	636:647	non-fat milk	636:647	Tryptic digest products from several standard proteins and two real samples (non-fat milk and serum) were enriched using Ti-CTS to demonstrate the efficiency of this method.
26680323	6	60	theme	*	969:969	arg1	*					973:973	S*S*S*	968:973	S*S*S*	968:973	Furthermore, multi-phosphorylated peptides with three serine phospholated sites (S*S*S*) demonstrated high affinity to Ti-CTS.
26680323	6	60	theme	*	969:969	arg1	sites					961:965	three serine phospholated sites	935:965	three serine phospholated sites (S*S*S*)	935:974	Furthermore, multi-phosphorylated peptides with three serine phospholated sites (S*S*S*) demonstrated high affinity to Ti-CTS.
26680323	0	61	theme	cross-linked	58:69	arg1	composite					80:88	titanium(IV) cross-linked chitosan composite	45:88	titanium(IV) cross-linked chitosan composite	45:88	Highly specific phosphopeptide enrichment by titanium(IV) cross-linked chitosan composite.
26680323	6	62	theme	*	971:971	arg1	*					973:973	S*S*S*	968:973	S*S*S*	968:973	Furthermore, multi-phosphorylated peptides with three serine phospholated sites (S*S*S*) demonstrated high affinity to Ti-CTS.
26680323	6	62	theme	*	971:971	arg1	sites					961:965	three serine phospholated sites	935:965	three serine phospholated sites (S*S*S*)	935:974	Furthermore, multi-phosphorylated peptides with three serine phospholated sites (S*S*S*) demonstrated high affinity to Ti-CTS.
26680323	7	63	theme	phosphoproteomes	1083:1098	arg1	studies					1064:1070	future studies	1057:1070	future studies of complex phosphoproteomes and especially multi-phosphorylated peptides	1057:1143	Hence, this method had great potential for future studies of complex phosphoproteomes and especially multi-phosphorylated peptides.
26680323	4	64	theme	standard	596:603	arg1	proteins					605:612	several standard proteins	588:612	several standard proteins	588:612	Tryptic digest products from several standard proteins and two real samples (non-fat milk and serum) were enriched using Ti-CTS to demonstrate the efficiency of this method.
26680323	1	65	theme	material	214:221	arg1	Ti-CTS					224:229	Ti(IV) based immobilized metal ion affinity chromatographic (IMAC) material (Ti-CTS)	147:230	Ti(IV) based immobilized metal ion affinity chromatographic (IMAC) material (Ti-CTS)	147:230	Natural chitosan was applied as supporting material for Ti(IV) based immobilized metal ion affinity chromatographic (IMAC) material (Ti-CTS).
26680323	4	66	theme	method	725:730	arg1	efficiency					706:715	the efficiency	702:715	the efficiency of this method	702:730	Tryptic digest products from several standard proteins and two real samples (non-fat milk and serum) were enriched using Ti-CTS to demonstrate the efficiency of this method.
26680323	1	67	theme	Ti	147:148	arg1	Ti-CTS					224:229	Ti(IV) based immobilized metal ion affinity chromatographic (IMAC) material (Ti-CTS)	147:230	Ti(IV) based immobilized metal ion affinity chromatographic (IMAC) material (Ti-CTS)	147:230	Natural chitosan was applied as supporting material for Ti(IV) based immobilized metal ion affinity chromatographic (IMAC) material (Ti-CTS).
28818383	2	0	theme	biomass	446:452	arg1	digestibility					454:466	biomass digestibility	446:466	biomass digestibility	446:466	Here we explored the effects of the stress-response hormones, salicylic acid and methyl jasmonate, on cell wall biosynthesis and biomass digestibility in Brachypodium distachyon, a species recently considered as a suitable model for biomass conversion.
28818383	1	1	theme	cellulosic	297:306	arg1	ethanol					308:314	cellulosic ethanol	297:314	cellulosic ethanol	297:314	In addition to playing a key role in the response to environmental changes, cell walls are also considered as a valuable feedstock for cellulosic ethanol.
28818383	6	2	theme	expression	1066:1075	arg1	pattern					1077:1083	the expression pattern	1062:1083	the expression pattern	1062:1083	The hormonal profile and the expression pattern of genes involved in cell wall biosynthesis were also modified.
28818383	1	3	theme	environmental	215:227	arg1	changes					229:235	environmental changes	215:235	environmental changes	215:235	In addition to playing a key role in the response to environmental changes, cell walls are also considered as a valuable feedstock for cellulosic ethanol.
28818383	8	4	theme	wall	1467:1470	arg1	digestibility					1472:1484	cell wall digestibility	1462:1484	cell wall digestibility	1462:1484	The results obtained here aid in our understanding of cell wall dynamics in response to stress and will enable the development of new strategies to improve cell wall digestibility in bioenergy feedstock.
28818383	3	5	theme	plant	645:649	arg1	growth					651:656	plant growth	645:656	plant growth	645:656	We found that in response to salicylic acid or methyl jasmonate treatment, plant growth was reduced coupled with significant changes in cell wall composition.
28818383	8	6	theme	cell	1462:1465	arg1	digestibility					1472:1484	cell wall digestibility	1462:1484	cell wall digestibility	1462:1484	The results obtained here aid in our understanding of cell wall dynamics in response to stress and will enable the development of new strategies to improve cell wall digestibility in bioenergy feedstock.
28818383	3	7	theme	salicylic	599:607	arg1	acid					609:612	salicylic acid	599:612	salicylic acid	599:612	We found that in response to salicylic acid or methyl jasmonate treatment, plant growth was reduced coupled with significant changes in cell wall composition.
28818383	8	8	theme	cell	1360:1363	arg1	dynamics					1370:1377	cell wall dynamics	1360:1377	cell wall dynamics	1360:1377	The results obtained here aid in our understanding of cell wall dynamics in response to stress and will enable the development of new strategies to improve cell wall digestibility in bioenergy feedstock.
28818383	0	9	theme	salicylic	74:82	arg1	acid					84:87	only salicylic acid	69:87	only salicylic acid	69:87	Methyl jasmonate and salicylic acid are able to modify cell wall but only salicylic acid alters biomass digestibility in the model grass Brachypodium distachyon.
28818383	4	10	theme	acid	853:856	arg1	application					858:868	salicylic acid application	843:868	salicylic acid application	843:868	Cellulose content increased in response to methyl jasmonate whereas a reduction in lignin content was found after salicylic acid application.
28818383	4	11	theme	Cellulose	729:737	arg1	content					739:745	Cellulose content	729:745	Cellulose content	729:745	Cellulose content increased in response to methyl jasmonate whereas a reduction in lignin content was found after salicylic acid application.
28818383	6	12	theme	wall	1111:1114	arg1	biosynthesis					1116:1127	cell wall biosynthesis	1106:1127	cell wall biosynthesis	1106:1127	The hormonal profile and the expression pattern of genes involved in cell wall biosynthesis were also modified.
28818383	7	13	theme	leaf	1186:1189	arg1	tissue					1191:1196	leaf tissue	1186:1196	leaf tissue	1186:1196	Biomass digestibility was reduced in leaf tissue after salicylic acid treatment and was negatively correlated with ferulic acid and p-coumaric acid content.
28818383	3	14	theme	significant	683:693	arg1	changes					695:701	significant changes	683:701	significant changes in cell wall composition	683:726	We found that in response to salicylic acid or methyl jasmonate treatment, plant growth was reduced coupled with significant changes in cell wall composition.
28818383	2	15	theme	biomass	550:556	arg1	conversion					558:567	biomass conversion	550:567	biomass conversion	550:567	Here we explored the effects of the stress-response hormones, salicylic acid and methyl jasmonate, on cell wall biosynthesis and biomass digestibility in Brachypodium distachyon, a species recently considered as a suitable model for biomass conversion.
28818383	1	16	theme	cell	238:241	arg1	walls					243:247	cell walls	238:247	cell walls	238:247	In addition to playing a key role in the response to environmental changes, cell walls are also considered as a valuable feedstock for cellulosic ethanol.
28818383	1	16	theme	cell	238:241	arg1	feedstock					283:291	a valuable feedstock	272:291	a valuable feedstock for cellulosic ethanol	272:314	In addition to playing a key role in the response to environmental changes, cell walls are also considered as a valuable feedstock for cellulosic ethanol.
28818383	6	17	theme	cell	1106:1109	arg1	biosynthesis					1116:1127	cell wall biosynthesis	1106:1127	cell wall biosynthesis	1106:1127	The hormonal profile and the expression pattern of genes involved in cell wall biosynthesis were also modified.
28818383	4	18	theme	lignin	812:817	arg1	content					819:825	lignin content	812:825	lignin content	812:825	Cellulose content increased in response to methyl jasmonate whereas a reduction in lignin content was found after salicylic acid application.
28818383	5	19	theme	ferulic	950:956	arg1	acid					958:961	ferulic acid	950:961	ferulic acid	950:961	Moreover, hemicellulose composition was altered and increases in caffeic acid, ferulic acid and p-coumaric acid content were detected in response to both treatments.
28818383	2	20	theme	stress-response	353:367	arg1	jasmonate					405:413	methyl jasmonate	398:413	methyl jasmonate	398:413	Here we explored the effects of the stress-response hormones, salicylic acid and methyl jasmonate, on cell wall biosynthesis and biomass digestibility in Brachypodium distachyon, a species recently considered as a suitable model for biomass conversion.
28818383	2	20	theme	stress-response	353:367	arg1	hormones					369:376	the stress-response hormones	349:376	the stress-response hormones	349:376	Here we explored the effects of the stress-response hormones, salicylic acid and methyl jasmonate, on cell wall biosynthesis and biomass digestibility in Brachypodium distachyon, a species recently considered as a suitable model for biomass conversion.
28818383	2	20	theme	stress-response	353:367	arg1	acid					389:392	salicylic acid	379:392	salicylic acid	379:392	Here we explored the effects of the stress-response hormones, salicylic acid and methyl jasmonate, on cell wall biosynthesis and biomass digestibility in Brachypodium distachyon, a species recently considered as a suitable model for biomass conversion.
28818383	2	21	theme	Brachypodium	471:482	arg1	distachyon					484:493	Brachypodium distachyon	471:493	Brachypodium distachyon	471:493	Here we explored the effects of the stress-response hormones, salicylic acid and methyl jasmonate, on cell wall biosynthesis and biomass digestibility in Brachypodium distachyon, a species recently considered as a suitable model for biomass conversion.
28818383	2	21	theme	Brachypodium	471:482	arg1	species					498:504	a species	496:504	a species recently considered as a suitable model for biomass conversion	496:567	Here we explored the effects of the stress-response hormones, salicylic acid and methyl jasmonate, on cell wall biosynthesis and biomass digestibility in Brachypodium distachyon, a species recently considered as a suitable model for biomass conversion.
28818383	2	22	from	biosynthesis	429:440	arg1	distachyon					484:493	Brachypodium distachyon	471:493	Brachypodium distachyon	471:493	Here we explored the effects of the stress-response hormones, salicylic acid and methyl jasmonate, on cell wall biosynthesis and biomass digestibility in Brachypodium distachyon, a species recently considered as a suitable model for biomass conversion.
28818383	2	22	from	biosynthesis	429:440	arg1	species					498:504	a species	496:504	a species recently considered as a suitable model for biomass conversion	496:567	Here we explored the effects of the stress-response hormones, salicylic acid and methyl jasmonate, on cell wall biosynthesis and biomass digestibility in Brachypodium distachyon, a species recently considered as a suitable model for biomass conversion.
28818383	7	23	theme	Biomass	1149:1155	arg1	digestibility					1157:1169	Biomass digestibility	1149:1169	Biomass digestibility	1149:1169	Biomass digestibility was reduced in leaf tissue after salicylic acid treatment and was negatively correlated with ferulic acid and p-coumaric acid content.
28818383	3	24	dep	reduced	662:668	arg1	coupled					670:676	coupled	670:676	coupled with significant changes in cell wall composition	670:726	We found that in response to salicylic acid or methyl jasmonate treatment, plant growth was reduced coupled with significant changes in cell wall composition.
28818383	5	25	theme	acid	978:981	arg1	content					983:989	p-coumaric acid content	967:989	p-coumaric acid content	967:989	Moreover, hemicellulose composition was altered and increases in caffeic acid, ferulic acid and p-coumaric acid content were detected in response to both treatments.
28818383	6	26	theme	hormonal	1041:1048	arg1	profile					1050:1056	The hormonal profile	1037:1056	The hormonal profile	1037:1056	The hormonal profile and the expression pattern of genes involved in cell wall biosynthesis were also modified.
28818383	0	27	theme	Methyl	0:5	arg1	jasmonate					7:15	Methyl jasmonate	0:15	Methyl jasmonate	0:15	Methyl jasmonate and salicylic acid are able to modify cell wall but only salicylic acid alters biomass digestibility in the model grass Brachypodium distachyon.
28818383	3	28	theme	wall	711:714	arg1	composition					716:726	cell wall composition	706:726	cell wall composition	706:726	We found that in response to salicylic acid or methyl jasmonate treatment, plant growth was reduced coupled with significant changes in cell wall composition.
28818383	0	29	theme	salicylic	21:29	arg1	acid					31:34	salicylic acid	21:34	salicylic acid	21:34	Methyl jasmonate and salicylic acid are able to modify cell wall but only salicylic acid alters biomass digestibility in the model grass Brachypodium distachyon.
28818383	4	30	theme	methyl	772:777	arg1	jasmonate					779:787	methyl jasmonate	772:787	methyl jasmonate	772:787	Cellulose content increased in response to methyl jasmonate whereas a reduction in lignin content was found after salicylic acid application.
28818383	8	31	theme	wall	1365:1368	arg1	dynamics					1370:1377	cell wall dynamics	1360:1377	cell wall dynamics	1360:1377	The results obtained here aid in our understanding of cell wall dynamics in response to stress and will enable the development of new strategies to improve cell wall digestibility in bioenergy feedstock.
28818383	7	32	theme	acid	1292:1295	arg1	content					1297:1303	p-coumaric acid content	1281:1303	p-coumaric acid content	1281:1303	Biomass digestibility was reduced in leaf tissue after salicylic acid treatment and was negatively correlated with ferulic acid and p-coumaric acid content.
28818383	3	33	from	changes	695:701	arg1	composition					716:726	cell wall composition	706:726	cell wall composition	706:726	We found that in response to salicylic acid or methyl jasmonate treatment, plant growth was reduced coupled with significant changes in cell wall composition.
28818383	0	34	theme	biomass	96:102	arg1	digestibility					104:116	biomass digestibility	96:116	biomass digestibility	96:116	Methyl jasmonate and salicylic acid are able to modify cell wall but only salicylic acid alters biomass digestibility in the model grass Brachypodium distachyon.
28818383	5	35	theme	caffeic	936:942	arg1	acid					944:947	caffeic acid	936:947	caffeic acid	936:947	Moreover, hemicellulose composition was altered and increases in caffeic acid, ferulic acid and p-coumaric acid content were detected in response to both treatments.
28818383	2	36	theme	salicylic	379:387	arg1	hormones					369:376	the stress-response hormones	349:376	the stress-response hormones	349:376	Here we explored the effects of the stress-response hormones, salicylic acid and methyl jasmonate, on cell wall biosynthesis and biomass digestibility in Brachypodium distachyon, a species recently considered as a suitable model for biomass conversion.
28818383	2	36	theme	salicylic	379:387	arg1	acid					389:392	salicylic acid	379:392	salicylic acid	379:392	Here we explored the effects of the stress-response hormones, salicylic acid and methyl jasmonate, on cell wall biosynthesis and biomass digestibility in Brachypodium distachyon, a species recently considered as a suitable model for biomass conversion.
28818383	7	37	theme	p-coumaric	1281:1290	arg1	content					1297:1303	p-coumaric acid content	1281:1303	p-coumaric acid content	1281:1303	Biomass digestibility was reduced in leaf tissue after salicylic acid treatment and was negatively correlated with ferulic acid and p-coumaric acid content.
28818383	3	38	theme	cell	706:709	arg1	composition					716:726	cell wall composition	706:726	cell wall composition	706:726	We found that in response to salicylic acid or methyl jasmonate treatment, plant growth was reduced coupled with significant changes in cell wall composition.
28818383	1	39	theme	key	187:189	arg1	role					191:194	a key role	185:194	a key role	185:194	In addition to playing a key role in the response to environmental changes, cell walls are also considered as a valuable feedstock for cellulosic ethanol.
28818383	2	40	theme	wall	424:427	arg1	biosynthesis					429:440	cell wall biosynthesis	419:440	cell wall biosynthesis	419:440	Here we explored the effects of the stress-response hormones, salicylic acid and methyl jasmonate, on cell wall biosynthesis and biomass digestibility in Brachypodium distachyon, a species recently considered as a suitable model for biomass conversion.
28818383	8	41	theme	new	1436:1438	arg1	strategies					1440:1449	new strategies	1436:1449	new strategies	1436:1449	The results obtained here aid in our understanding of cell wall dynamics in response to stress and will enable the development of new strategies to improve cell wall digestibility in bioenergy feedstock.
28818383	2	42	theme	methyl	398:403	arg1	jasmonate					405:413	methyl jasmonate	398:413	methyl jasmonate	398:413	Here we explored the effects of the stress-response hormones, salicylic acid and methyl jasmonate, on cell wall biosynthesis and biomass digestibility in Brachypodium distachyon, a species recently considered as a suitable model for biomass conversion.
28818383	2	42	theme	methyl	398:403	arg1	hormones					369:376	the stress-response hormones	349:376	the stress-response hormones	349:376	Here we explored the effects of the stress-response hormones, salicylic acid and methyl jasmonate, on cell wall biosynthesis and biomass digestibility in Brachypodium distachyon, a species recently considered as a suitable model for biomass conversion.
28818383	4	43	from	reduction	799:807	arg1	content					819:825	lignin content	812:825	lignin content	812:825	Cellulose content increased in response to methyl jasmonate whereas a reduction in lignin content was found after salicylic acid application.
28818383	2	44	theme	hormones	369:376	arg1	effects					338:344	the effects	334:344	the effects of the stress-response hormones, salicylic acid and methyl jasmonate, on cell wall biosynthesis and biomass digestibility in Brachypodium distachyon, a species recently considered as a suitable model for biomass conversion	334:567	Here we explored the effects of the stress-response hormones, salicylic acid and methyl jasmonate, on cell wall biosynthesis and biomass digestibility in Brachypodium distachyon, a species recently considered as a suitable model for biomass conversion.
28818383	2	45	theme	cell	419:422	arg1	biosynthesis					429:440	cell wall biosynthesis	419:440	cell wall biosynthesis	419:440	Here we explored the effects of the stress-response hormones, salicylic acid and methyl jasmonate, on cell wall biosynthesis and biomass digestibility in Brachypodium distachyon, a species recently considered as a suitable model for biomass conversion.
28818383	0	46	theme	grass	131:135	arg1	distachyon					150:159	the model grass Brachypodium distachyon	121:159	the model grass Brachypodium distachyon	121:159	Methyl jasmonate and salicylic acid are able to modify cell wall but only salicylic acid alters biomass digestibility in the model grass Brachypodium distachyon.
28818383	2	47	from	digestibility	454:466	arg1	distachyon					484:493	Brachypodium distachyon	471:493	Brachypodium distachyon	471:493	Here we explored the effects of the stress-response hormones, salicylic acid and methyl jasmonate, on cell wall biosynthesis and biomass digestibility in Brachypodium distachyon, a species recently considered as a suitable model for biomass conversion.
28818383	2	47	from	digestibility	454:466	arg1	species					498:504	a species	496:504	a species recently considered as a suitable model for biomass conversion	496:567	Here we explored the effects of the stress-response hormones, salicylic acid and methyl jasmonate, on cell wall biosynthesis and biomass digestibility in Brachypodium distachyon, a species recently considered as a suitable model for biomass conversion.
28818383	2	48	from	effects	338:344	arg1	digestibility					454:466	biomass digestibility	446:466	biomass digestibility	446:466	Here we explored the effects of the stress-response hormones, salicylic acid and methyl jasmonate, on cell wall biosynthesis and biomass digestibility in Brachypodium distachyon, a species recently considered as a suitable model for biomass conversion.
28818383	2	48	from	effects	338:344	arg1	biosynthesis					429:440	cell wall biosynthesis	419:440	cell wall biosynthesis	419:440	Here we explored the effects of the stress-response hormones, salicylic acid and methyl jasmonate, on cell wall biosynthesis and biomass digestibility in Brachypodium distachyon, a species recently considered as a suitable model for biomass conversion.
28818383	5	49	theme	hemicellulose	881:893	arg1	composition					895:905	hemicellulose composition	881:905	hemicellulose composition	881:905	Moreover, hemicellulose composition was altered and increases in caffeic acid, ferulic acid and p-coumaric acid content were detected in response to both treatments.
28818383	1	50	theme	valuable	274:281	arg1	walls					243:247	cell walls	238:247	cell walls	238:247	In addition to playing a key role in the response to environmental changes, cell walls are also considered as a valuable feedstock for cellulosic ethanol.
28818383	1	50	theme	valuable	274:281	arg1	feedstock					283:291	a valuable feedstock	272:291	a valuable feedstock for cellulosic ethanol	272:314	In addition to playing a key role in the response to environmental changes, cell walls are also considered as a valuable feedstock for cellulosic ethanol.
28818383	0	51	theme	model	125:129	arg1	distachyon					150:159	the model grass Brachypodium distachyon	121:159	the model grass Brachypodium distachyon	121:159	Methyl jasmonate and salicylic acid are able to modify cell wall but only salicylic acid alters biomass digestibility in the model grass Brachypodium distachyon.
28818383	7	52	theme	ferulic	1264:1270	arg1	acid					1272:1275	ferulic acid	1264:1275	ferulic acid	1264:1275	Biomass digestibility was reduced in leaf tissue after salicylic acid treatment and was negatively correlated with ferulic acid and p-coumaric acid content.
28818383	7	53	theme	salicylic	1204:1212	arg1	acid					1214:1217	salicylic acid	1204:1217	salicylic acid treatment	1204:1227	Biomass digestibility was reduced in leaf tissue after salicylic acid treatment and was negatively correlated with ferulic acid and p-coumaric acid content.
28818383	2	54	theme	suitable	531:538	arg1	model					540:544	a suitable model	529:544	a suitable model for biomass conversion	529:567	Here we explored the effects of the stress-response hormones, salicylic acid and methyl jasmonate, on cell wall biosynthesis and biomass digestibility in Brachypodium distachyon, a species recently considered as a suitable model for biomass conversion.
28818383	8	55	theme	strategies	1440:1449	arg1	development					1421:1431	the development	1417:1431	the development of new strategies	1417:1449	The results obtained here aid in our understanding of cell wall dynamics in response to stress and will enable the development of new strategies to improve cell wall digestibility in bioenergy feedstock.
28818383	8	56	theme	dynamics	1370:1377	arg1	understanding					1343:1355	our understanding	1339:1355	our understanding of cell wall dynamics	1339:1377	The results obtained here aid in our understanding of cell wall dynamics in response to stress and will enable the development of new strategies to improve cell wall digestibility in bioenergy feedstock.
28818383	5	57	located	detected	996:1003	arg2	increases					923:931	increases	923:931	increases in caffeic acid, ferulic acid and p-coumaric acid content	923:989	Moreover, hemicellulose composition was altered and increases in caffeic acid, ferulic acid and p-coumaric acid content were detected in response to both treatments.
28818383	5	57	located	detected	996:1003	arg1	response					1008:1015	response	1008:1015	response to both treatments	1008:1034	Moreover, hemicellulose composition was altered and increases in caffeic acid, ferulic acid and p-coumaric acid content were detected in response to both treatments.
28818383	0	58	theme	Brachypodium	137:148	arg1	distachyon					150:159	the model grass Brachypodium distachyon	121:159	the model grass Brachypodium distachyon	121:159	Methyl jasmonate and salicylic acid are able to modify cell wall but only salicylic acid alters biomass digestibility in the model grass Brachypodium distachyon.
28818383	3	59	theme	methyl	617:622	arg1	treatment					634:642	methyl jasmonate treatment	617:642	methyl jasmonate treatment	617:642	We found that in response to salicylic acid or methyl jasmonate treatment, plant growth was reduced coupled with significant changes in cell wall composition.
28818383	5	60	theme	p-coumaric	967:976	arg1	content					983:989	p-coumaric acid content	967:989	p-coumaric acid content	967:989	Moreover, hemicellulose composition was altered and increases in caffeic acid, ferulic acid and p-coumaric acid content were detected in response to both treatments.
28818383	6	61	theme	genes	1088:1092	arg1	pattern					1077:1083	the expression pattern	1062:1083	the expression pattern	1062:1083	The hormonal profile and the expression pattern of genes involved in cell wall biosynthesis were also modified.
28818383	6	61	theme	genes	1088:1092	arg1	profile					1050:1056	The hormonal profile	1037:1056	The hormonal profile	1037:1056	The hormonal profile and the expression pattern of genes involved in cell wall biosynthesis were also modified.
28818383	4	62	theme	salicylic	843:851	arg1	application					858:868	salicylic acid application	843:868	salicylic acid application	843:868	Cellulose content increased in response to methyl jasmonate whereas a reduction in lignin content was found after salicylic acid application.
28818383	0	63	theme	cell	55:58	arg1	wall					60:63	cell wall	55:63	cell wall	55:63	Methyl jasmonate and salicylic acid are able to modify cell wall but only salicylic acid alters biomass digestibility in the model grass Brachypodium distachyon.
28818383	3	64	theme	jasmonate	624:632	arg1	treatment					634:642	methyl jasmonate treatment	617:642	methyl jasmonate treatment	617:642	We found that in response to salicylic acid or methyl jasmonate treatment, plant growth was reduced coupled with significant changes in cell wall composition.
28818383	1	65	dep	playing	177:183	arg1	addition					165:172	addition	165:172	addition	165:172	In addition to playing a key role in the response to environmental changes, cell walls are also considered as a valuable feedstock for cellulosic ethanol.
28818383	5	66	from	increases	923:931	arg1	content					983:989	p-coumaric acid content	967:989	p-coumaric acid content	967:989	Moreover, hemicellulose composition was altered and increases in caffeic acid, ferulic acid and p-coumaric acid content were detected in response to both treatments.
28818383	5	66	from	increases	923:931	arg1	acid					944:947	caffeic acid	936:947	caffeic acid	936:947	Moreover, hemicellulose composition was altered and increases in caffeic acid, ferulic acid and p-coumaric acid content were detected in response to both treatments.
28818383	5	66	from	increases	923:931	arg1	acid					958:961	ferulic acid	950:961	ferulic acid	950:961	Moreover, hemicellulose composition was altered and increases in caffeic acid, ferulic acid and p-coumaric acid content were detected in response to both treatments.
28818383	3	67	dep	acid	609:612	arg1	response					587:594	response	587:594	response	587:594	We found that in response to salicylic acid or methyl jasmonate treatment, plant growth was reduced coupled with significant changes in cell wall composition.
28818383	7	68	theme	acid	1214:1217	arg1	treatment					1219:1227	salicylic acid treatment	1204:1227	salicylic acid treatment	1204:1227	Biomass digestibility was reduced in leaf tissue after salicylic acid treatment and was negatively correlated with ferulic acid and p-coumaric acid content.
28818383	4	69	dep	jasmonate	779:787	arg1	response					760:767	response	760:767	response	760:767	Cellulose content increased in response to methyl jasmonate whereas a reduction in lignin content was found after salicylic acid application.
28818383	8	70	theme	bioenergy	1489:1497	arg1	feedstock					1499:1507	bioenergy feedstock	1489:1507	bioenergy feedstock	1489:1507	The results obtained here aid in our understanding of cell wall dynamics in response to stress and will enable the development of new strategies to improve cell wall digestibility in bioenergy feedstock.
27312609	8	0	theme	random	1200:1205	arg1	conformation					1212:1223	random coil conformation	1200:1223	random coil conformation	1200:1223	Smaller molecular size (root-mean square radius, Rz 20.2nm) and approximated spherical conformation in 100kGy-KGM enhanced its interaction with TMP and maintained its compact and smooth structure, but the larger molecular size (Rz≥40.2nm) and random coil conformation in other KGMs inhibited part of actins from gelling and deteriorated the network structure.
27312609	8	1	from	conformation	1212:1223	arg1	KGMs					1234:1237	other KGMs	1228:1237	other KGMs	1228:1237	Smaller molecular size (root-mean square radius, Rz 20.2nm) and approximated spherical conformation in 100kGy-KGM enhanced its interaction with TMP and maintained its compact and smooth structure, but the larger molecular size (Rz≥40.2nm) and random coil conformation in other KGMs inhibited part of actins from gelling and deteriorated the network structure.
27312609	8	2	from	size	975:978	arg1	100kGy-KGM					1060:1069	100kGy-KGM	1060:1069	100kGy-KGM	1060:1069	Smaller molecular size (root-mean square radius, Rz 20.2nm) and approximated spherical conformation in 100kGy-KGM enhanced its interaction with TMP and maintained its compact and smooth structure, but the larger molecular size (Rz≥40.2nm) and random coil conformation in other KGMs inhibited part of actins from gelling and deteriorated the network structure.
27312609	8	3	with	interaction	1084:1094	arg1	TMP					1101:1103	TMP	1101:1103	TMP	1101:1103	Smaller molecular size (root-mean square radius, Rz 20.2nm) and approximated spherical conformation in 100kGy-KGM enhanced its interaction with TMP and maintained its compact and smooth structure, but the larger molecular size (Rz≥40.2nm) and random coil conformation in other KGMs inhibited part of actins from gelling and deteriorated the network structure.
27312609	9	4	theme	structure-functions	1374:1392	arg1	relationships					1394:1406	the structure-functions relationships	1370:1406	the structure-functions relationships of KGM-TMP composite gels	1370:1432	Our study provided principle knowledge to understand the structure-functions relationships of KGM-TMP composite gels.
27312609	0	5	theme	rheological	73:83	arg1	properties					85:94	rheological properties	73:94	rheological properties	73:94	Effect of molecular characteristics of Konjac glucomannan on gelling and rheological properties of Tilapia myofibrillar protein.
27312609	8	6	theme	square	991:996	arg1	radius					998:1003	root-mean square radius	981:1003	root-mean square radius	981:1003	Smaller molecular size (root-mean square radius, Rz 20.2nm) and approximated spherical conformation in 100kGy-KGM enhanced its interaction with TMP and maintained its compact and smooth structure, but the larger molecular size (Rz≥40.2nm) and random coil conformation in other KGMs inhibited part of actins from gelling and deteriorated the network structure.
27312609	8	6	theme	square	991:996	arg1	20.2nm					1009:1014	Rz 20.2nm	1006:1014	Rz 20.2nm	1006:1014	Smaller molecular size (root-mean square radius, Rz 20.2nm) and approximated spherical conformation in 100kGy-KGM enhanced its interaction with TMP and maintained its compact and smooth structure, but the larger molecular size (Rz≥40.2nm) and random coil conformation in other KGMs inhibited part of actins from gelling and deteriorated the network structure.
27312609	7	7	theme	molecular	924:932	arg1	size					934:937	different molecular size	914:937	different molecular size	914:937	These behaviors or interactions are resulted from different molecular size and conformation.
27312609	3	8	theme	native	465:470	arg1	KGM					472:474	native KGM	465:474	native KGM	465:474	In this work, TMP composite gels were prepared under neutral pH with varying KGM (native KGM, 10kGy-KGM, 20kGy-KGM, and 100kGy-KGM) of different molecular characteristics.
27312609	0	9	theme	Tilapia	99:105	arg1	protein					120:126	Tilapia myofibrillar protein	99:126	Tilapia myofibrillar protein	99:126	Effect of molecular characteristics of Konjac glucomannan on gelling and rheological properties of Tilapia myofibrillar protein.
27312609	8	10	theme	molecular	965:973	arg1	size					975:978	Smaller molecular size	957:978	Smaller molecular size (root-mean square radius, Rz 20.2nm)	957:1015	Smaller molecular size (root-mean square radius, Rz 20.2nm) and approximated spherical conformation in 100kGy-KGM enhanced its interaction with TMP and maintained its compact and smooth structure, but the larger molecular size (Rz≥40.2nm) and random coil conformation in other KGMs inhibited part of actins from gelling and deteriorated the network structure.
27312609	8	11	theme	Rz	1006:1007	arg1	radius					998:1003	root-mean square radius	981:1003	root-mean square radius	981:1003	Smaller molecular size (root-mean square radius, Rz 20.2nm) and approximated spherical conformation in 100kGy-KGM enhanced its interaction with TMP and maintained its compact and smooth structure, but the larger molecular size (Rz≥40.2nm) and random coil conformation in other KGMs inhibited part of actins from gelling and deteriorated the network structure.
27312609	8	11	theme	Rz	1006:1007	arg1	20.2nm					1009:1014	Rz 20.2nm	1006:1014	Rz 20.2nm	1006:1014	Smaller molecular size (root-mean square radius, Rz 20.2nm) and approximated spherical conformation in 100kGy-KGM enhanced its interaction with TMP and maintained its compact and smooth structure, but the larger molecular size (Rz≥40.2nm) and random coil conformation in other KGMs inhibited part of actins from gelling and deteriorated the network structure.
27312609	8	12	from	part	1249:1252	arg1	gelling					1269:1275	gelling	1269:1275	gelling	1269:1275	Smaller molecular size (root-mean square radius, Rz 20.2nm) and approximated spherical conformation in 100kGy-KGM enhanced its interaction with TMP and maintained its compact and smooth structure, but the larger molecular size (Rz≥40.2nm) and random coil conformation in other KGMs inhibited part of actins from gelling and deteriorated the network structure.
27312609	2	13	theme	molecular	281:289	arg1	characteristics					264:278	KGM molecular characteristics	250:278	KGM molecular characteristics (molecular weight, size and conformation)	250:320	This study aimed to investigate the effects of KGM molecular characteristics (molecular weight, size and conformation) on gelling properties of Tilapia myofibrillar protein (TMP).
27312609	2	13	theme	molecular	281:289	arg1	weight					291:296	molecular weight	281:296	molecular weight	281:296	This study aimed to investigate the effects of KGM molecular characteristics (molecular weight, size and conformation) on gelling properties of Tilapia myofibrillar protein (TMP).
27312609	8	14	theme	compact	1124:1130	arg1	structure					1143:1151	its compact and smooth structure	1120:1151	its compact and smooth structure	1120:1151	Smaller molecular size (root-mean square radius, Rz 20.2nm) and approximated spherical conformation in 100kGy-KGM enhanced its interaction with TMP and maintained its compact and smooth structure, but the larger molecular size (Rz≥40.2nm) and random coil conformation in other KGMs inhibited part of actins from gelling and deteriorated the network structure.
27312609	8	15	theme	Smaller	957:963	arg1	size					975:978	Smaller molecular size	957:978	Smaller molecular size (root-mean square radius, Rz 20.2nm)	957:1015	Smaller molecular size (root-mean square radius, Rz 20.2nm) and approximated spherical conformation in 100kGy-KGM enhanced its interaction with TMP and maintained its compact and smooth structure, but the larger molecular size (Rz≥40.2nm) and random coil conformation in other KGMs inhibited part of actins from gelling and deteriorated the network structure.
27312609	9	16	theme	KGM-TMP	1411:1417	arg1	gels					1429:1432	KGM-TMP composite gels	1411:1432	KGM-TMP composite gels	1411:1432	Our study provided principle knowledge to understand the structure-functions relationships of KGM-TMP composite gels.
27312609	0	17	theme	protein	120:126	arg1	gelling					61:67	gelling	61:67	gelling	61:67	Effect of molecular characteristics of Konjac glucomannan on gelling and rheological properties of Tilapia myofibrillar protein.
27312609	0	17	theme	protein	120:126	arg1	properties					85:94	rheological properties	73:94	rheological properties	73:94	Effect of molecular characteristics of Konjac glucomannan on gelling and rheological properties of Tilapia myofibrillar protein.
27312609	4	18	theme	gels	652:655	arg1	whiteness					635:643	whiteness	635:643	whiteness	635:643	Native KGM, 10kGy-KGM, and 20kGy-KGM exerted negative effect on gel strength or whiteness of TMP gels.
27312609	4	18	theme	gels	652:655	arg1	strength					623:630	gel strength	619:630	gel strength	619:630	Native KGM, 10kGy-KGM, and 20kGy-KGM exerted negative effect on gel strength or whiteness of TMP gels.
27312609	3	19	theme	neutral	436:442	arg1	pH					444:445	neutral pH	436:445	neutral pH	436:445	In this work, TMP composite gels were prepared under neutral pH with varying KGM (native KGM, 10kGy-KGM, 20kGy-KGM, and 100kGy-KGM) of different molecular characteristics.
27312609	2	20	theme	characteristics	264:278	arg1	effects					239:245	the effects	235:245	the effects of KGM molecular characteristics (molecular weight, size and conformation) on gelling properties of Tilapia myofibrillar protein (TMP)	235:380	This study aimed to investigate the effects of KGM molecular characteristics (molecular weight, size and conformation) on gelling properties of Tilapia myofibrillar protein (TMP).
27312609	8	21	theme	coil	1207:1210	arg1	conformation					1212:1223	random coil conformation	1200:1223	random coil conformation	1200:1223	Smaller molecular size (root-mean square radius, Rz 20.2nm) and approximated spherical conformation in 100kGy-KGM enhanced its interaction with TMP and maintained its compact and smooth structure, but the larger molecular size (Rz≥40.2nm) and random coil conformation in other KGMs inhibited part of actins from gelling and deteriorated the network structure.
27312609	1	22	theme	important	160:168	arg1	agent					178:182	an important gelling agent	157:182	an important gelling agent in composite gels	157:200	Konjac glucomannan (KGM) is an important gelling agent in composite gels.
27312609	1	22	theme	important	160:168	arg1	glucomannan					136:146	Konjac glucomannan	129:146	Konjac glucomannan (KGM)	129:152	Konjac glucomannan (KGM) is an important gelling agent in composite gels.
27312609	0	23	theme	myofibrillar	107:118	arg1	protein					120:126	Tilapia myofibrillar protein	99:126	Tilapia myofibrillar protein	99:126	Effect of molecular characteristics of Konjac glucomannan on gelling and rheological properties of Tilapia myofibrillar protein.
27312609	8	24	from	size	1179:1182	arg1	KGMs					1234:1237	other KGMs	1228:1237	other KGMs	1228:1237	Smaller molecular size (root-mean square radius, Rz 20.2nm) and approximated spherical conformation in 100kGy-KGM enhanced its interaction with TMP and maintained its compact and smooth structure, but the larger molecular size (Rz≥40.2nm) and random coil conformation in other KGMs inhibited part of actins from gelling and deteriorated the network structure.
27312609	5	25	theme	gels	732:735	arg1	whiteness					715:723	whiteness	715:723	whiteness	715:723	Interestingly 100kGy-KGM improved gelling properties and whiteness of TMP gels.
27312609	5	25	theme	gels	732:735	arg1	properties					700:709	properties	700:709	properties	700:709	Interestingly 100kGy-KGM improved gelling properties and whiteness of TMP gels.
27312609	2	26	theme	molecular	254:262	arg1	conformation					308:319	conformation	308:319	conformation	308:319	This study aimed to investigate the effects of KGM molecular characteristics (molecular weight, size and conformation) on gelling properties of Tilapia myofibrillar protein (TMP).
27312609	2	26	theme	molecular	254:262	arg1	size					299:302	size	299:302	size	299:302	This study aimed to investigate the effects of KGM molecular characteristics (molecular weight, size and conformation) on gelling properties of Tilapia myofibrillar protein (TMP).
27312609	2	26	theme	molecular	254:262	arg1	characteristics					264:278	KGM molecular characteristics	250:278	KGM molecular characteristics (molecular weight, size and conformation)	250:320	This study aimed to investigate the effects of KGM molecular characteristics (molecular weight, size and conformation) on gelling properties of Tilapia myofibrillar protein (TMP).
27312609	2	26	theme	molecular	254:262	arg1	weight					291:296	molecular weight	281:296	molecular weight	281:296	This study aimed to investigate the effects of KGM molecular characteristics (molecular weight, size and conformation) on gelling properties of Tilapia myofibrillar protein (TMP).
27312609	8	27	dep	size	975:978	arg1	radius					998:1003	root-mean square radius	981:1003	root-mean square radius	981:1003	Smaller molecular size (root-mean square radius, Rz 20.2nm) and approximated spherical conformation in 100kGy-KGM enhanced its interaction with TMP and maintained its compact and smooth structure, but the larger molecular size (Rz≥40.2nm) and random coil conformation in other KGMs inhibited part of actins from gelling and deteriorated the network structure.
27312609	8	27	dep	size	975:978	arg1	20.2nm					1009:1014	Rz 20.2nm	1006:1014	Rz 20.2nm	1006:1014	Smaller molecular size (root-mean square radius, Rz 20.2nm) and approximated spherical conformation in 100kGy-KGM enhanced its interaction with TMP and maintained its compact and smooth structure, but the larger molecular size (Rz≥40.2nm) and random coil conformation in other KGMs inhibited part of actins from gelling and deteriorated the network structure.
27312609	1	28	theme	gelling	170:176	arg1	agent					178:182	an important gelling agent	157:182	an important gelling agent in composite gels	157:200	Konjac glucomannan (KGM) is an important gelling agent in composite gels.
27312609	1	28	theme	gelling	170:176	arg1	glucomannan					136:146	Konjac glucomannan	129:146	Konjac glucomannan (KGM)	129:152	Konjac glucomannan (KGM) is an important gelling agent in composite gels.
27312609	5	29	theme	TMP	728:730	arg1	gels					732:735	TMP gels	728:735	TMP gels	728:735	Interestingly 100kGy-KGM improved gelling properties and whiteness of TMP gels.
27312609	8	30	theme	approximated	1021:1032	arg1	conformation					1044:1055	approximated spherical conformation	1021:1055	approximated spherical conformation	1021:1055	Smaller molecular size (root-mean square radius, Rz 20.2nm) and approximated spherical conformation in 100kGy-KGM enhanced its interaction with TMP and maintained its compact and smooth structure, but the larger molecular size (Rz≥40.2nm) and random coil conformation in other KGMs inhibited part of actins from gelling and deteriorated the network structure.
27312609	6	31	theme	Such	738:741	arg1	effects					743:749	Such effects	738:749	Such effects presented by varying KGM	738:774	Such effects presented by varying KGM were attributed the physical filling behaviors and the interaction between KGM and TMP.
27312609	4	32	theme	gel	619:621	arg1	strength					623:630	gel strength	619:630	gel strength	619:630	Native KGM, 10kGy-KGM, and 20kGy-KGM exerted negative effect on gel strength or whiteness of TMP gels.
27312609	8	33	theme	root-mean	981:989	arg1	radius					998:1003	root-mean square radius	981:1003	root-mean square radius	981:1003	Smaller molecular size (root-mean square radius, Rz 20.2nm) and approximated spherical conformation in 100kGy-KGM enhanced its interaction with TMP and maintained its compact and smooth structure, but the larger molecular size (Rz≥40.2nm) and random coil conformation in other KGMs inhibited part of actins from gelling and deteriorated the network structure.
27312609	8	33	theme	root-mean	981:989	arg1	20.2nm					1009:1014	Rz 20.2nm	1006:1014	Rz 20.2nm	1006:1014	Smaller molecular size (root-mean square radius, Rz 20.2nm) and approximated spherical conformation in 100kGy-KGM enhanced its interaction with TMP and maintained its compact and smooth structure, but the larger molecular size (Rz≥40.2nm) and random coil conformation in other KGMs inhibited part of actins from gelling and deteriorated the network structure.
27312609	2	34	from	effects	239:245	arg1	properties					333:342	gelling properties	325:342	gelling properties of Tilapia myofibrillar protein (TMP)	325:380	This study aimed to investigate the effects of KGM molecular characteristics (molecular weight, size and conformation) on gelling properties of Tilapia myofibrillar protein (TMP).
27312609	2	35	theme	protein	368:374	arg1	properties					333:342	gelling properties	325:342	gelling properties of Tilapia myofibrillar protein (TMP)	325:380	This study aimed to investigate the effects of KGM molecular characteristics (molecular weight, size and conformation) on gelling properties of Tilapia myofibrillar protein (TMP).
27312609	0	36	theme	characteristics	20:34	arg1	Effect					0:5	Effect	0:5	Effect of molecular characteristics of Konjac	0:44	Effect of molecular characteristics of Konjac glucomannan on gelling and rheological properties of Tilapia myofibrillar protein.
27312609	8	37	from	gelling	1269:1275	arg1	actins					1257:1262	actins	1257:1262	actins from gelling	1257:1275	Smaller molecular size (root-mean square radius, Rz 20.2nm) and approximated spherical conformation in 100kGy-KGM enhanced its interaction with TMP and maintained its compact and smooth structure, but the larger molecular size (Rz≥40.2nm) and random coil conformation in other KGMs inhibited part of actins from gelling and deteriorated the network structure.
27312609	8	37	from	gelling	1269:1275	arg1	part					1249:1252	part	1249:1252	part of actins from gelling	1249:1275	Smaller molecular size (root-mean square radius, Rz 20.2nm) and approximated spherical conformation in 100kGy-KGM enhanced its interaction with TMP and maintained its compact and smooth structure, but the larger molecular size (Rz≥40.2nm) and random coil conformation in other KGMs inhibited part of actins from gelling and deteriorated the network structure.
27312609	7	38	theme	different	914:922	arg1	size					934:937	different molecular size	914:937	different molecular size	914:937	These behaviors or interactions are resulted from different molecular size and conformation.
27312609	1	39	theme	composite	187:195	arg1	gels					197:200	composite gels	187:200	composite gels	187:200	Konjac glucomannan (KGM) is an important gelling agent in composite gels.
27312609	0	40	theme	molecular	10:18	arg1	characteristics					20:34	molecular characteristics	10:34	molecular characteristics of Konjac	10:44	Effect of molecular characteristics of Konjac glucomannan on gelling and rheological properties of Tilapia myofibrillar protein.
27312609	8	41	theme	spherical	1034:1042	arg1	conformation					1044:1055	approximated spherical conformation	1021:1055	approximated spherical conformation	1021:1055	Smaller molecular size (root-mean square radius, Rz 20.2nm) and approximated spherical conformation in 100kGy-KGM enhanced its interaction with TMP and maintained its compact and smooth structure, but the larger molecular size (Rz≥40.2nm) and random coil conformation in other KGMs inhibited part of actins from gelling and deteriorated the network structure.
27312609	10	42	used	used	1456:1459	arg2	results					1441:1447	These results	1435:1447	These results	1435:1447	These results can be used to provide theoretical guidance for surimi gel processing.
27312609	2	43	theme	myofibrillar	355:366	arg1	TMP					377:379	TMP	377:379	TMP	377:379	This study aimed to investigate the effects of KGM molecular characteristics (molecular weight, size and conformation) on gelling properties of Tilapia myofibrillar protein (TMP).
27312609	2	43	theme	myofibrillar	355:366	arg1	protein					368:374	Tilapia myofibrillar protein	347:374	Tilapia myofibrillar protein (TMP)	347:380	This study aimed to investigate the effects of KGM molecular characteristics (molecular weight, size and conformation) on gelling properties of Tilapia myofibrillar protein (TMP).
27312609	0	44	theme	Konjac	39:44	arg1	characteristics					20:34	molecular characteristics	10:34	molecular characteristics of Konjac	10:44	Effect of molecular characteristics of Konjac glucomannan on gelling and rheological properties of Tilapia myofibrillar protein.
27312609	4	45	theme	TMP	648:650	arg1	gels					652:655	TMP gels	648:655	TMP gels	648:655	Native KGM, 10kGy-KGM, and 20kGy-KGM exerted negative effect on gel strength or whiteness of TMP gels.
27312609	3	46	theme	characteristics	538:552	arg1	KGM					460:462	KGM	460:462	KGM (native KGM, 10kGy-KGM, 20kGy-KGM, and 100kGy-KGM) of different molecular characteristics	460:552	In this work, TMP composite gels were prepared under neutral pH with varying KGM (native KGM, 10kGy-KGM, 20kGy-KGM, and 100kGy-KGM) of different molecular characteristics.
27312609	3	47	theme	TMP	397:399	arg1	gels					411:414	TMP composite gels	397:414	TMP composite gels	397:414	In this work, TMP composite gels were prepared under neutral pH with varying KGM (native KGM, 10kGy-KGM, 20kGy-KGM, and 100kGy-KGM) of different molecular characteristics.
27312609	3	48	theme	composite	401:409	arg1	gels					411:414	TMP composite gels	397:414	TMP composite gels	397:414	In this work, TMP composite gels were prepared under neutral pH with varying KGM (native KGM, 10kGy-KGM, 20kGy-KGM, and 100kGy-KGM) of different molecular characteristics.
27312609	8	49	theme	molecular	1169:1177	arg1	size					1179:1182	the larger molecular size	1158:1182	the larger molecular size (Rz≥40.2nm)	1158:1194	Smaller molecular size (root-mean square radius, Rz 20.2nm) and approximated spherical conformation in 100kGy-KGM enhanced its interaction with TMP and maintained its compact and smooth structure, but the larger molecular size (Rz≥40.2nm) and random coil conformation in other KGMs inhibited part of actins from gelling and deteriorated the network structure.
27312609	8	49	theme	molecular	1169:1177	arg1	Rz≥40.2nm					1185:1193	Rz≥40.2nm	1185:1193	Rz≥40.2nm	1185:1193	Smaller molecular size (root-mean square radius, Rz 20.2nm) and approximated spherical conformation in 100kGy-KGM enhanced its interaction with TMP and maintained its compact and smooth structure, but the larger molecular size (Rz≥40.2nm) and random coil conformation in other KGMs inhibited part of actins from gelling and deteriorated the network structure.
27312609	8	50	theme	larger	1162:1167	arg1	size					1179:1182	the larger molecular size	1158:1182	the larger molecular size (Rz≥40.2nm)	1158:1194	Smaller molecular size (root-mean square radius, Rz 20.2nm) and approximated spherical conformation in 100kGy-KGM enhanced its interaction with TMP and maintained its compact and smooth structure, but the larger molecular size (Rz≥40.2nm) and random coil conformation in other KGMs inhibited part of actins from gelling and deteriorated the network structure.
27312609	8	50	theme	larger	1162:1167	arg1	Rz≥40.2nm					1185:1193	Rz≥40.2nm	1185:1193	Rz≥40.2nm	1185:1193	Smaller molecular size (root-mean square radius, Rz 20.2nm) and approximated spherical conformation in 100kGy-KGM enhanced its interaction with TMP and maintained its compact and smooth structure, but the larger molecular size (Rz≥40.2nm) and random coil conformation in other KGMs inhibited part of actins from gelling and deteriorated the network structure.
27312609	8	51	theme	smooth	1136:1141	arg1	structure					1143:1151	its compact and smooth structure	1120:1151	its compact and smooth structure	1120:1151	Smaller molecular size (root-mean square radius, Rz 20.2nm) and approximated spherical conformation in 100kGy-KGM enhanced its interaction with TMP and maintained its compact and smooth structure, but the larger molecular size (Rz≥40.2nm) and random coil conformation in other KGMs inhibited part of actins from gelling and deteriorated the network structure.
27312609	9	52	theme	gels	1429:1432	arg1	relationships					1394:1406	the structure-functions relationships	1370:1406	the structure-functions relationships of KGM-TMP composite gels	1370:1432	Our study provided principle knowledge to understand the structure-functions relationships of KGM-TMP composite gels.
27312609	6	53	theme	filling	805:811	arg1	behaviors					813:821	the physical filling behaviors	792:821	the physical filling behaviors	792:821	Such effects presented by varying KGM were attributed the physical filling behaviors and the interaction between KGM and TMP.
27312609	3	54	theme	different	518:526	arg1	characteristics					538:552	different molecular characteristics	518:552	different molecular characteristics	518:552	In this work, TMP composite gels were prepared under neutral pH with varying KGM (native KGM, 10kGy-KGM, 20kGy-KGM, and 100kGy-KGM) of different molecular characteristics.
27312609	9	55	theme	composite	1419:1427	arg1	gels					1429:1432	KGM-TMP composite gels	1411:1432	KGM-TMP composite gels	1411:1432	Our study provided principle knowledge to understand the structure-functions relationships of KGM-TMP composite gels.
27312609	2	56	theme	gelling	325:331	arg1	properties					333:342	gelling properties	325:342	gelling properties of Tilapia myofibrillar protein (TMP)	325:380	This study aimed to investigate the effects of KGM molecular characteristics (molecular weight, size and conformation) on gelling properties of Tilapia myofibrillar protein (TMP).
27312609	10	57	theme	gel	1504:1506	arg1	processing					1508:1517	surimi gel processing	1497:1517	surimi gel processing	1497:1517	These results can be used to provide theoretical guidance for surimi gel processing.
27312609	6	58	theme	physical	796:803	arg1	behaviors					813:821	the physical filling behaviors	792:821	the physical filling behaviors	792:821	Such effects presented by varying KGM were attributed the physical filling behaviors and the interaction between KGM and TMP.
27312609	3	59	theme	molecular	528:536	arg1	characteristics					538:552	different molecular characteristics	518:552	different molecular characteristics	518:552	In this work, TMP composite gels were prepared under neutral pH with varying KGM (native KGM, 10kGy-KGM, 20kGy-KGM, and 100kGy-KGM) of different molecular characteristics.
27312609	1	60	theme	Konjac	129:134	arg1	glucomannan					136:146	Konjac glucomannan	129:146	Konjac glucomannan (KGM)	129:152	Konjac glucomannan (KGM) is an important gelling agent in composite gels.
27312609	1	60	theme	Konjac	129:134	arg1	agent					178:182	an important gelling agent	157:182	an important gelling agent in composite gels	157:200	Konjac glucomannan (KGM) is an important gelling agent in composite gels.
27312609	1	60	theme	Konjac	129:134	arg1	KGM					149:151	KGM	149:151	KGM	149:151	Konjac glucomannan (KGM) is an important gelling agent in composite gels.
27312609	9	61	theme	principle	1336:1344	arg1	knowledge					1346:1354	principle knowledge	1336:1354	principle knowledge	1336:1354	Our study provided principle knowledge to understand the structure-functions relationships of KGM-TMP composite gels.
27312609	10	62	theme	surimi	1497:1502	arg1	processing					1508:1517	surimi gel processing	1497:1517	surimi gel processing	1497:1517	These results can be used to provide theoretical guidance for surimi gel processing.
27312609	2	63	theme	KGM	250:252	arg1	conformation					308:319	conformation	308:319	conformation	308:319	This study aimed to investigate the effects of KGM molecular characteristics (molecular weight, size and conformation) on gelling properties of Tilapia myofibrillar protein (TMP).
27312609	2	63	theme	KGM	250:252	arg1	size					299:302	size	299:302	size	299:302	This study aimed to investigate the effects of KGM molecular characteristics (molecular weight, size and conformation) on gelling properties of Tilapia myofibrillar protein (TMP).
27312609	2	63	theme	KGM	250:252	arg1	characteristics					264:278	KGM molecular characteristics	250:278	KGM molecular characteristics (molecular weight, size and conformation)	250:320	This study aimed to investigate the effects of KGM molecular characteristics (molecular weight, size and conformation) on gelling properties of Tilapia myofibrillar protein (TMP).
27312609	2	63	theme	KGM	250:252	arg1	weight					291:296	molecular weight	281:296	molecular weight	281:296	This study aimed to investigate the effects of KGM molecular characteristics (molecular weight, size and conformation) on gelling properties of Tilapia myofibrillar protein (TMP).
27312609	8	64	theme	actins	1257:1262	arg1	part					1249:1252	part	1249:1252	part of actins from gelling	1249:1275	Smaller molecular size (root-mean square radius, Rz 20.2nm) and approximated spherical conformation in 100kGy-KGM enhanced its interaction with TMP and maintained its compact and smooth structure, but the larger molecular size (Rz≥40.2nm) and random coil conformation in other KGMs inhibited part of actins from gelling and deteriorated the network structure.
27312609	3	65	dep	KGM	460:462	arg1	20kGy-KGM					488:496	20kGy-KGM	488:496	20kGy-KGM	488:496	In this work, TMP composite gels were prepared under neutral pH with varying KGM (native KGM, 10kGy-KGM, 20kGy-KGM, and 100kGy-KGM) of different molecular characteristics.
27312609	3	65	dep	KGM	460:462	arg1	100kGy-KGM					503:512	100kGy-KGM	503:512	100kGy-KGM	503:512	In this work, TMP composite gels were prepared under neutral pH with varying KGM (native KGM, 10kGy-KGM, 20kGy-KGM, and 100kGy-KGM) of different molecular characteristics.
27312609	3	65	dep	KGM	460:462	arg1	10kGy-KGM					477:485	10kGy-KGM	477:485	10kGy-KGM	477:485	In this work, TMP composite gels were prepared under neutral pH with varying KGM (native KGM, 10kGy-KGM, 20kGy-KGM, and 100kGy-KGM) of different molecular characteristics.
27312609	3	65	dep	KGM	460:462	arg1	KGM					472:474	native KGM	465:474	native KGM	465:474	In this work, TMP composite gels were prepared under neutral pH with varying KGM (native KGM, 10kGy-KGM, 20kGy-KGM, and 100kGy-KGM) of different molecular characteristics.
27312609	8	66	theme	network	1298:1304	arg1	structure					1306:1314	the network structure	1294:1314	the network structure	1294:1314	Smaller molecular size (root-mean square radius, Rz 20.2nm) and approximated spherical conformation in 100kGy-KGM enhanced its interaction with TMP and maintained its compact and smooth structure, but the larger molecular size (Rz≥40.2nm) and random coil conformation in other KGMs inhibited part of actins from gelling and deteriorated the network structure.
27312609	1	67	from	agent	178:182	arg1	gels					197:200	composite gels	187:200	composite gels	187:200	Konjac glucomannan (KGM) is an important gelling agent in composite gels.
27312609	4	68	theme	Native	555:560	arg1	KGM					562:564	Native KGM	555:564	Native KGM	555:564	Native KGM, 10kGy-KGM, and 20kGy-KGM exerted negative effect on gel strength or whiteness of TMP gels.
27312609	10	69	theme	theoretical	1472:1482	arg1	guidance					1484:1491	theoretical guidance	1472:1491	theoretical guidance for surimi gel processing	1472:1517	These results can be used to provide theoretical guidance for surimi gel processing.
27312609	8	70	theme	other	1228:1232	arg1	KGMs					1234:1237	other KGMs	1228:1237	other KGMs	1228:1237	Smaller molecular size (root-mean square radius, Rz 20.2nm) and approximated spherical conformation in 100kGy-KGM enhanced its interaction with TMP and maintained its compact and smooth structure, but the larger molecular size (Rz≥40.2nm) and random coil conformation in other KGMs inhibited part of actins from gelling and deteriorated the network structure.
27312609	2	71	dep	characteristics	264:278	arg1	conformation					308:319	conformation	308:319	conformation	308:319	This study aimed to investigate the effects of KGM molecular characteristics (molecular weight, size and conformation) on gelling properties of Tilapia myofibrillar protein (TMP).
27312609	2	71	dep	characteristics	264:278	arg1	size					299:302	size	299:302	size	299:302	This study aimed to investigate the effects of KGM molecular characteristics (molecular weight, size and conformation) on gelling properties of Tilapia myofibrillar protein (TMP).
27312609	2	71	dep	characteristics	264:278	arg1	characteristics					264:278	KGM molecular characteristics	250:278	KGM molecular characteristics (molecular weight, size and conformation)	250:320	This study aimed to investigate the effects of KGM molecular characteristics (molecular weight, size and conformation) on gelling properties of Tilapia myofibrillar protein (TMP).
27312609	2	71	dep	characteristics	264:278	arg1	weight					291:296	molecular weight	281:296	molecular weight	281:296	This study aimed to investigate the effects of KGM molecular characteristics (molecular weight, size and conformation) on gelling properties of Tilapia myofibrillar protein (TMP).
27312609	4	72	theme	negative	600:607	arg1	effect					609:614	negative effect	600:614	negative effect	600:614	Native KGM, 10kGy-KGM, and 20kGy-KGM exerted negative effect on gel strength or whiteness of TMP gels.
27312609	8	73	from	conformation	1044:1055	arg1	100kGy-KGM					1060:1069	100kGy-KGM	1060:1069	100kGy-KGM	1060:1069	Smaller molecular size (root-mean square radius, Rz 20.2nm) and approximated spherical conformation in 100kGy-KGM enhanced its interaction with TMP and maintained its compact and smooth structure, but the larger molecular size (Rz≥40.2nm) and random coil conformation in other KGMs inhibited part of actins from gelling and deteriorated the network structure.
27312609	2	74	theme	Tilapia	347:353	arg1	TMP					377:379	TMP	377:379	TMP	377:379	This study aimed to investigate the effects of KGM molecular characteristics (molecular weight, size and conformation) on gelling properties of Tilapia myofibrillar protein (TMP).
27312609	2	74	theme	Tilapia	347:353	arg1	protein					368:374	Tilapia myofibrillar protein	347:374	Tilapia myofibrillar protein (TMP)	347:380	This study aimed to investigate the effects of KGM molecular characteristics (molecular weight, size and conformation) on gelling properties of Tilapia myofibrillar protein (TMP).
26791584	5	0	theme	complex	644:650	arg1	built					661:665	a complex glycitol built	642:665	a complex glycitol built in hexose polymerization	642:690	MALDI-TOF mass spectroscopy also demonstrated that PAL is a complex glycitol built in hexose polymerization.
26791584	5	0	theme	complex	644:650	arg1	PAL					635:637	PAL	635:637	PAL	635:637	MALDI-TOF mass spectroscopy also demonstrated that PAL is a complex glycitol built in hexose polymerization.
26791584	8	1	theme	pathway	1252:1258	arg1	activation					1203:1212	the activation	1199:1212	the activation of MAPKs (ERK, p38 and JNK) signaling pathway	1199:1258	These results clearly demonstrate that PAL stimulates the immune response in RAW 264.7 cells through the activation of MAPKs (ERK, p38 and JNK) signaling pathway.
26791584	4	2	theme	groups	576:581	arg1	amount					557:562	significant amount	545:562	significant amount of hydroxyl groups	545:581	Fourier-transform infrared (FT-IR) analysis indicated that the purified PAL is a complex glycitol, which structurally contains significant amount of hydroxyl groups.
26791584	4	2	theme	groups	576:581	arg1	groups					576:581	hydroxyl groups	567:581	hydroxyl groups	567:581	Fourier-transform infrared (FT-IR) analysis indicated that the purified PAL is a complex glycitol, which structurally contains significant amount of hydroxyl groups.
26791584	4	3	theme	complex	499:505	arg1	glycitol					507:514	a complex glycitol	497:514	a complex glycitol	497:514	Fourier-transform infrared (FT-IR) analysis indicated that the purified PAL is a complex glycitol, which structurally contains significant amount of hydroxyl groups.
26791584	4	3	theme	complex	499:505	arg1	PAL					490:492	the purified PAL	477:492	the purified PAL	477:492	Fourier-transform infrared (FT-IR) analysis indicated that the purified PAL is a complex glycitol, which structurally contains significant amount of hydroxyl groups.
26791584	2	4	theme	primary	197:203	arg1	glucose					264:270	glucose	264:270	glucose	264:270	The primary monosaccharide composition of the PAL was determined to be glucose, where HPAEC analysis showed no significant amount of any other sugars.
26791584	2	4	theme	primary	197:203	arg1	composition					220:230	The primary monosaccharide composition	193:230	The primary monosaccharide composition of the PAL	193:241	The primary monosaccharide composition of the PAL was determined to be glucose, where HPAEC analysis showed no significant amount of any other sugars.
26791584	5	5	theme	mass	594:597	arg1	spectroscopy					599:610	MALDI-TOF mass spectroscopy	584:610	MALDI-TOF mass spectroscopy	584:610	MALDI-TOF mass spectroscopy also demonstrated that PAL is a complex glycitol built in hexose polymerization.
26791584	1	6	theme	immunostimulatory	149:165	arg1	activities					167:176	its immunostimulatory activities	145:176	its immunostimulatory activities	145:176	A polyhydric alcohol (PAL) was isolated from Taxus cuspidata and its immunostimulatory activities were assessed.
26791584	7	7	theme	cells	882:886	arg1	treatment					859:867	treatment	859:867	treatment of RAW 264.7 cells with PAL for 24h	859:903	Furthermore, treatment of RAW 264.7 cells with PAL for 24h remarkably increased the phosphorylation levels of ERK, p38 and JNK in a dose-dependent manner, whereas the total protein levels of ERK (t-ERK), p38 (t-p38) and JNK (t-JNK) remained unchanged.
26791584	4	8	theme	hydroxyl	567:574	arg1	groups					576:581	hydroxyl groups	567:581	hydroxyl groups	567:581	Fourier-transform infrared (FT-IR) analysis indicated that the purified PAL is a complex glycitol, which structurally contains significant amount of hydroxyl groups.
26791584	7	9	theme	JNK	969:971	arg1	levels					946:951	the phosphorylation levels	926:951	the phosphorylation levels of ERK, p38 and JNK	926:971	Furthermore, treatment of RAW 264.7 cells with PAL for 24h remarkably increased the phosphorylation levels of ERK, p38 and JNK in a dose-dependent manner, whereas the total protein levels of ERK (t-ERK), p38 (t-p38) and JNK (t-JNK) remained unchanged.
26791584	7	10	theme	JNK	1066:1068	arg1	levels					1027:1032	the total protein levels	1009:1032	the total protein levels of ERK (t-ERK), p38 (t-p38) and JNK (t-JNK)	1009:1076	Furthermore, treatment of RAW 264.7 cells with PAL for 24h remarkably increased the phosphorylation levels of ERK, p38 and JNK in a dose-dependent manner, whereas the total protein levels of ERK (t-ERK), p38 (t-p38) and JNK (t-JNK) remained unchanged.
26791584	7	11	dep	increased	916:924	arg1	whereas					1001:1007	whereas	1001:1007	whereas	1001:1007	Furthermore, treatment of RAW 264.7 cells with PAL for 24h remarkably increased the phosphorylation levels of ERK, p38 and JNK in a dose-dependent manner, whereas the total protein levels of ERK (t-ERK), p38 (t-p38) and JNK (t-JNK) remained unchanged.
26791584	2	12	theme	HPAEC	279:283	arg1	analysis					285:292	HPAEC analysis	279:292	HPAEC analysis	279:292	The primary monosaccharide composition of the PAL was determined to be glucose, where HPAEC analysis showed no significant amount of any other sugars.
26791584	6	13	dep	cytokines	793:801	arg1	TNF-α					803:807	TNF-α	803:807	TNF-α	803:807	Enzyme linked immunosorbent assay showed that the PAL stimulates the release of the proinflammatory cytokines TNF-α and IL-6 in a dose-dependent manner.
26791584	6	13	dep	cytokines	793:801	arg1	cytokines					793:801	the proinflammatory cytokines	773:801	the proinflammatory cytokines TNF-α and IL-6	773:816	Enzyme linked immunosorbent assay showed that the PAL stimulates the release of the proinflammatory cytokines TNF-α and IL-6 in a dose-dependent manner.
26791584	6	13	dep	cytokines	793:801	arg1	IL-6					813:816	IL-6	813:816	IL-6	813:816	Enzyme linked immunosorbent assay showed that the PAL stimulates the release of the proinflammatory cytokines TNF-α and IL-6 in a dose-dependent manner.
26791584	9	14	theme	T.	1411:1412	arg1	cuspidata					1414:1422	T. cuspidata	1411:1422	T. cuspidata	1411:1422	To the best of our knowledge, this is the first study to demonstrate the primary structure and immune-stimulating activities of PAL from the fruit of T. cuspidata.
26791584	7	15	theme	RAW	872:874	arg1	cells					882:886	RAW 264.7 cells	872:886	RAW 264.7 cells	872:886	Furthermore, treatment of RAW 264.7 cells with PAL for 24h remarkably increased the phosphorylation levels of ERK, p38 and JNK in a dose-dependent manner, whereas the total protein levels of ERK (t-ERK), p38 (t-p38) and JNK (t-JNK) remained unchanged.
26791584	7	16	theme	p38	961:963	arg1	levels					946:951	the phosphorylation levels	926:951	the phosphorylation levels of ERK, p38 and JNK	926:971	Furthermore, treatment of RAW 264.7 cells with PAL for 24h remarkably increased the phosphorylation levels of ERK, p38 and JNK in a dose-dependent manner, whereas the total protein levels of ERK (t-ERK), p38 (t-p38) and JNK (t-JNK) remained unchanged.
26791584	9	17	from	fruit	1402:1406	arg1	structure					1342:1350	primary structure	1334:1350	primary structure	1334:1350	To the best of our knowledge, this is the first study to demonstrate the primary structure and immune-stimulating activities of PAL from the fruit of T. cuspidata.
26791584	9	17	from	fruit	1402:1406	arg1	activities					1375:1384	immune-stimulating activities	1356:1384	immune-stimulating activities	1356:1384	To the best of our knowledge, this is the first study to demonstrate the primary structure and immune-stimulating activities of PAL from the fruit of T. cuspidata.
26791584	9	18	theme	primary	1334:1340	arg1	structure					1342:1350	primary structure	1334:1350	primary structure	1334:1350	To the best of our knowledge, this is the first study to demonstrate the primary structure and immune-stimulating activities of PAL from the fruit of T. cuspidata.
26791584	4	19	theme	purified	481:488	arg1	glycitol					507:514	a complex glycitol	497:514	a complex glycitol	497:514	Fourier-transform infrared (FT-IR) analysis indicated that the purified PAL is a complex glycitol, which structurally contains significant amount of hydroxyl groups.
26791584	4	19	theme	purified	481:488	arg1	PAL					490:492	the purified PAL	477:492	the purified PAL	477:492	Fourier-transform infrared (FT-IR) analysis indicated that the purified PAL is a complex glycitol, which structurally contains significant amount of hydroxyl groups.
26791584	1	20	theme	polyhydric	82:91	arg1	PAL					102:104	PAL	102:104	PAL	102:104	A polyhydric alcohol (PAL) was isolated from Taxus cuspidata and its immunostimulatory activities were assessed.
26791584	1	20	theme	polyhydric	82:91	arg1	alcohol					93:99	A polyhydric alcohol	80:99	A polyhydric alcohol (PAL)	80:105	A polyhydric alcohol (PAL) was isolated from Taxus cuspidata and its immunostimulatory activities were assessed.
26791584	6	21	theme	immunosorbent	707:719	arg1	assay					721:725	Enzyme linked immunosorbent assay	693:725	Enzyme linked immunosorbent assay	693:725	Enzyme linked immunosorbent assay showed that the PAL stimulates the release of the proinflammatory cytokines TNF-α and IL-6 in a dose-dependent manner.
26791584	7	22	theme	p38	1050:1052	arg1	levels					1027:1032	the total protein levels	1009:1032	the total protein levels of ERK (t-ERK), p38 (t-p38) and JNK (t-JNK)	1009:1076	Furthermore, treatment of RAW 264.7 cells with PAL for 24h remarkably increased the phosphorylation levels of ERK, p38 and JNK in a dose-dependent manner, whereas the total protein levels of ERK (t-ERK), p38 (t-p38) and JNK (t-JNK) remained unchanged.
26791584	7	23	theme	ERK	956:958	arg1	levels					946:951	the phosphorylation levels	926:951	the phosphorylation levels of ERK, p38 and JNK	926:971	Furthermore, treatment of RAW 264.7 cells with PAL for 24h remarkably increased the phosphorylation levels of ERK, p38 and JNK in a dose-dependent manner, whereas the total protein levels of ERK (t-ERK), p38 (t-p38) and JNK (t-JNK) remained unchanged.
26791584	6	24	theme	linked	700:705	arg1	assay					721:725	Enzyme linked immunosorbent assay	693:725	Enzyme linked immunosorbent assay	693:725	Enzyme linked immunosorbent assay showed that the PAL stimulates the release of the proinflammatory cytokines TNF-α and IL-6 in a dose-dependent manner.
26791584	0	25	theme	Immunostimulating	0:16	arg1	activity					18:25	Immunostimulating activity	0:25	Immunostimulating activity of polyhydric alcohol	0:47	Immunostimulating activity of polyhydric alcohol isolated from Taxus cuspidata.
26791584	4	26	theme	significant	545:555	arg1	amount					557:562	significant amount	545:562	significant amount of hydroxyl groups	545:581	Fourier-transform infrared (FT-IR) analysis indicated that the purified PAL is a complex glycitol, which structurally contains significant amount of hydroxyl groups.
26791584	4	26	theme	significant	545:555	arg1	groups					576:581	hydroxyl groups	567:581	hydroxyl groups	567:581	Fourier-transform infrared (FT-IR) analysis indicated that the purified PAL is a complex glycitol, which structurally contains significant amount of hydroxyl groups.
26791584	6	27	theme	cytokines	793:801	arg1	release					762:768	the release	758:768	the release of the proinflammatory cytokines TNF-α and IL-6	758:816	Enzyme linked immunosorbent assay showed that the PAL stimulates the release of the proinflammatory cytokines TNF-α and IL-6 in a dose-dependent manner.
26791584	2	28	theme	significant	304:314	arg1	sugars					336:341	any other sugars	326:341	any other sugars	326:341	The primary monosaccharide composition of the PAL was determined to be glucose, where HPAEC analysis showed no significant amount of any other sugars.
26791584	2	28	theme	significant	304:314	arg1	amount					316:321	no significant amount	301:321	no significant amount of any other sugars	301:341	The primary monosaccharide composition of the PAL was determined to be glucose, where HPAEC analysis showed no significant amount of any other sugars.
26791584	6	29	theme	Enzyme	693:698	arg1	assay					721:725	Enzyme linked immunosorbent assay	693:725	Enzyme linked immunosorbent assay	693:725	Enzyme linked immunosorbent assay showed that the PAL stimulates the release of the proinflammatory cytokines TNF-α and IL-6 in a dose-dependent manner.
26791584	5	30	theme	glycitol	652:659	arg1	built					661:665	a complex glycitol built	642:665	a complex glycitol built in hexose polymerization	642:690	MALDI-TOF mass spectroscopy also demonstrated that PAL is a complex glycitol built in hexose polymerization.
26791584	5	30	theme	glycitol	652:659	arg1	PAL					635:637	PAL	635:637	PAL	635:637	MALDI-TOF mass spectroscopy also demonstrated that PAL is a complex glycitol built in hexose polymerization.
26791584	0	31	theme	polyhydric	30:39	arg1	alcohol					41:47	polyhydric alcohol	30:47	polyhydric alcohol	30:47	Immunostimulating activity of polyhydric alcohol isolated from Taxus cuspidata.
26791584	7	32	theme	phosphorylation	930:944	arg1	levels					946:951	the phosphorylation levels	926:951	the phosphorylation levels of ERK, p38 and JNK	926:971	Furthermore, treatment of RAW 264.7 cells with PAL for 24h remarkably increased the phosphorylation levels of ERK, p38 and JNK in a dose-dependent manner, whereas the total protein levels of ERK (t-ERK), p38 (t-p38) and JNK (t-JNK) remained unchanged.
26791584	5	33	theme	MALDI-TOF	584:592	arg1	spectroscopy					599:610	MALDI-TOF mass spectroscopy	584:610	MALDI-TOF mass spectroscopy	584:610	MALDI-TOF mass spectroscopy also demonstrated that PAL is a complex glycitol built in hexose polymerization.
26791584	4	34	contain	contains	536:543	arg1	glycitol					507:514	a complex glycitol	497:514	a complex glycitol	497:514	Fourier-transform infrared (FT-IR) analysis indicated that the purified PAL is a complex glycitol, which structurally contains significant amount of hydroxyl groups.
26791584	4	34	contain	contains	536:543	arg2	amount					557:562	significant amount	545:562	significant amount of hydroxyl groups	545:581	Fourier-transform infrared (FT-IR) analysis indicated that the purified PAL is a complex glycitol, which structurally contains significant amount of hydroxyl groups.
26791584	4	34	contain	contains	536:543	arg1	PAL					490:492	the purified PAL	477:492	the purified PAL	477:492	Fourier-transform infrared (FT-IR) analysis indicated that the purified PAL is a complex glycitol, which structurally contains significant amount of hydroxyl groups.
26791584	4	34	contain	contains	536:543	arg2	groups					576:581	hydroxyl groups	567:581	hydroxyl groups	567:581	Fourier-transform infrared (FT-IR) analysis indicated that the purified PAL is a complex glycitol, which structurally contains significant amount of hydroxyl groups.
26791584	6	35	theme	dose-dependent	823:836	arg1	manner					838:843	a dose-dependent manner	821:843	a dose-dependent manner	821:843	Enzyme linked immunosorbent assay showed that the PAL stimulates the release of the proinflammatory cytokines TNF-α and IL-6 in a dose-dependent manner.
26791584	8	36	theme	signaling	1242:1250	arg1	pathway					1252:1258	MAPKs (ERK, p38 and JNK) signaling pathway	1217:1258	MAPKs (ERK, p38 and JNK) signaling pathway	1217:1258	These results clearly demonstrate that PAL stimulates the immune response in RAW 264.7 cells through the activation of MAPKs (ERK, p38 and JNK) signaling pathway.
26791584	3	37	theme	main	397:400	arg1	alcohols					408:415	the main sugar alcohols	393:415	the main sugar alcohols	393:415	However, glycerol and xylitol were identified as the main sugar alcohols.
26791584	3	37	theme	main	397:400	arg1	xylitol					366:372	xylitol	366:372	xylitol	366:372	However, glycerol and xylitol were identified as the main sugar alcohols.
26791584	3	37	theme	main	397:400	arg1	glycerol					353:360	glycerol	353:360	glycerol	353:360	However, glycerol and xylitol were identified as the main sugar alcohols.
26791584	8	38	from	response	1163:1170	arg1	cells					1185:1189	RAW 264.7 cells	1175:1189	RAW 264.7 cells	1175:1189	These results clearly demonstrate that PAL stimulates the immune response in RAW 264.7 cells through the activation of MAPKs (ERK, p38 and JNK) signaling pathway.
26791584	9	39	theme	immune-stimulating	1356:1373	arg1	activities					1375:1384	immune-stimulating activities	1356:1384	immune-stimulating activities	1356:1384	To the best of our knowledge, this is the first study to demonstrate the primary structure and immune-stimulating activities of PAL from the fruit of T. cuspidata.
26791584	9	40	theme	PAL	1389:1391	arg1	structure					1342:1350	primary structure	1334:1350	primary structure	1334:1350	To the best of our knowledge, this is the first study to demonstrate the primary structure and immune-stimulating activities of PAL from the fruit of T. cuspidata.
26791584	9	40	theme	PAL	1389:1391	arg1	activities					1375:1384	immune-stimulating activities	1356:1384	immune-stimulating activities	1356:1384	To the best of our knowledge, this is the first study to demonstrate the primary structure and immune-stimulating activities of PAL from the fruit of T. cuspidata.
26791584	3	41	theme	sugar	402:406	arg1	alcohols					408:415	the main sugar alcohols	393:415	the main sugar alcohols	393:415	However, glycerol and xylitol were identified as the main sugar alcohols.
26791584	3	41	theme	sugar	402:406	arg1	xylitol					366:372	xylitol	366:372	xylitol	366:372	However, glycerol and xylitol were identified as the main sugar alcohols.
26791584	3	41	theme	sugar	402:406	arg1	glycerol					353:360	glycerol	353:360	glycerol	353:360	However, glycerol and xylitol were identified as the main sugar alcohols.
26791584	5	42	from	built	661:665	arg1	polymerization					677:690	hexose polymerization	670:690	hexose polymerization	670:690	MALDI-TOF mass spectroscopy also demonstrated that PAL is a complex glycitol built in hexose polymerization.
26791584	0	43	theme	alcohol	41:47	arg1	activity					18:25	Immunostimulating activity	0:25	Immunostimulating activity of polyhydric alcohol	0:47	Immunostimulating activity of polyhydric alcohol isolated from Taxus cuspidata.
26791584	2	44	theme	PAL	239:241	arg1	glucose					264:270	glucose	264:270	glucose	264:270	The primary monosaccharide composition of the PAL was determined to be glucose, where HPAEC analysis showed no significant amount of any other sugars.
26791584	2	44	theme	PAL	239:241	arg1	composition					220:230	The primary monosaccharide composition	193:230	The primary monosaccharide composition of the PAL	193:241	The primary monosaccharide composition of the PAL was determined to be glucose, where HPAEC analysis showed no significant amount of any other sugars.
26791584	8	45	theme	MAPKs	1217:1221	arg1	pathway					1252:1258	MAPKs (ERK, p38 and JNK) signaling pathway	1217:1258	MAPKs (ERK, p38 and JNK) signaling pathway	1217:1258	These results clearly demonstrate that PAL stimulates the immune response in RAW 264.7 cells through the activation of MAPKs (ERK, p38 and JNK) signaling pathway.
26791584	0	46	theme	Taxus	63:67	arg1	cuspidata					69:77	Taxus cuspidata	63:77	Taxus cuspidata	63:77	Immunostimulating activity of polyhydric alcohol isolated from Taxus cuspidata.
26791584	5	47	theme	hexose	670:675	arg1	polymerization					677:690	hexose polymerization	670:690	hexose polymerization	670:690	MALDI-TOF mass spectroscopy also demonstrated that PAL is a complex glycitol built in hexose polymerization.
26791584	7	48	theme	total	1013:1017	arg1	levels					1027:1032	the total protein levels	1009:1032	the total protein levels of ERK (t-ERK), p38 (t-p38) and JNK (t-JNK)	1009:1076	Furthermore, treatment of RAW 264.7 cells with PAL for 24h remarkably increased the phosphorylation levels of ERK, p38 and JNK in a dose-dependent manner, whereas the total protein levels of ERK (t-ERK), p38 (t-p38) and JNK (t-JNK) remained unchanged.
26791584	2	49	theme	sugars	336:341	arg1	sugars					336:341	any other sugars	326:341	any other sugars	326:341	The primary monosaccharide composition of the PAL was determined to be glucose, where HPAEC analysis showed no significant amount of any other sugars.
26791584	2	49	theme	sugars	336:341	arg1	amount					316:321	no significant amount	301:321	no significant amount of any other sugars	301:341	The primary monosaccharide composition of the PAL was determined to be glucose, where HPAEC analysis showed no significant amount of any other sugars.
26791584	6	50	link	linked	700:705	arg1	assay					721:725	Enzyme linked immunosorbent assay	693:725	Enzyme linked immunosorbent assay	693:725	Enzyme linked immunosorbent assay showed that the PAL stimulates the release of the proinflammatory cytokines TNF-α and IL-6 in a dose-dependent manner.
26791584	7	51	theme	ERK	1037:1039	arg1	levels					1027:1032	the total protein levels	1009:1032	the total protein levels of ERK (t-ERK), p38 (t-p38) and JNK (t-JNK)	1009:1076	Furthermore, treatment of RAW 264.7 cells with PAL for 24h remarkably increased the phosphorylation levels of ERK, p38 and JNK in a dose-dependent manner, whereas the total protein levels of ERK (t-ERK), p38 (t-p38) and JNK (t-JNK) remained unchanged.
26791584	7	52	with	treatment	859:867	arg1	PAL					893:895	PAL	893:895	PAL	893:895	Furthermore, treatment of RAW 264.7 cells with PAL for 24h remarkably increased the phosphorylation levels of ERK, p38 and JNK in a dose-dependent manner, whereas the total protein levels of ERK (t-ERK), p38 (t-p38) and JNK (t-JNK) remained unchanged.
26791584	9	53	dep	structure	1342:1350	arg1	the					1330:1332	the	1330:1332	the	1330:1332	To the best of our knowledge, this is the first study to demonstrate the primary structure and immune-stimulating activities of PAL from the fruit of T. cuspidata.
26791584	2	54	theme	other	330:334	arg1	sugars					336:341	any other sugars	326:341	any other sugars	326:341	The primary monosaccharide composition of the PAL was determined to be glucose, where HPAEC analysis showed no significant amount of any other sugars.
26791584	6	55	theme	proinflammatory	777:791	arg1	TNF-α					803:807	TNF-α	803:807	TNF-α	803:807	Enzyme linked immunosorbent assay showed that the PAL stimulates the release of the proinflammatory cytokines TNF-α and IL-6 in a dose-dependent manner.
26791584	6	55	theme	proinflammatory	777:791	arg1	cytokines					793:801	the proinflammatory cytokines	773:801	the proinflammatory cytokines TNF-α and IL-6	773:816	Enzyme linked immunosorbent assay showed that the PAL stimulates the release of the proinflammatory cytokines TNF-α and IL-6 in a dose-dependent manner.
26791584	6	55	theme	proinflammatory	777:791	arg1	IL-6					813:816	IL-6	813:816	IL-6	813:816	Enzyme linked immunosorbent assay showed that the PAL stimulates the release of the proinflammatory cytokines TNF-α and IL-6 in a dose-dependent manner.
26791584	7	56	theme	protein	1019:1025	arg1	levels					1027:1032	the total protein levels	1009:1032	the total protein levels of ERK (t-ERK), p38 (t-p38) and JNK (t-JNK)	1009:1076	Furthermore, treatment of RAW 264.7 cells with PAL for 24h remarkably increased the phosphorylation levels of ERK, p38 and JNK in a dose-dependent manner, whereas the total protein levels of ERK (t-ERK), p38 (t-p38) and JNK (t-JNK) remained unchanged.
26791584	2	57	theme	monosaccharide	205:218	arg1	glucose					264:270	glucose	264:270	glucose	264:270	The primary monosaccharide composition of the PAL was determined to be glucose, where HPAEC analysis showed no significant amount of any other sugars.
26791584	2	57	theme	monosaccharide	205:218	arg1	composition					220:230	The primary monosaccharide composition	193:230	The primary monosaccharide composition of the PAL	193:241	The primary monosaccharide composition of the PAL was determined to be glucose, where HPAEC analysis showed no significant amount of any other sugars.
26791584	4	58	theme	infrared	436:443	arg1	FT-IR					446:450	FT-IR	446:450	FT-IR	446:450	Fourier-transform infrared (FT-IR) analysis indicated that the purified PAL is a complex glycitol, which structurally contains significant amount of hydroxyl groups.
26791584	4	58	theme	infrared	436:443	arg1	analysis					453:460	Fourier-transform infrared (FT-IR) analysis	418:460	Fourier-transform infrared (FT-IR) analysis	418:460	Fourier-transform infrared (FT-IR) analysis indicated that the purified PAL is a complex glycitol, which structurally contains significant amount of hydroxyl groups.
26791584	7	59	theme	dose-dependent	978:991	arg1	manner					993:998	a dose-dependent manner	976:998	a dose-dependent manner	976:998	Furthermore, treatment of RAW 264.7 cells with PAL for 24h remarkably increased the phosphorylation levels of ERK, p38 and JNK in a dose-dependent manner, whereas the total protein levels of ERK (t-ERK), p38 (t-p38) and JNK (t-JNK) remained unchanged.
26791584	1	60	theme	Taxus	125:129	arg1	cuspidata					131:139	Taxus cuspidata	125:139	Taxus cuspidata	125:139	A polyhydric alcohol (PAL) was isolated from Taxus cuspidata and its immunostimulatory activities were assessed.
26791584	8	61	theme	immune	1156:1161	arg1	response					1163:1170	the immune response	1152:1170	the immune response in RAW 264.7 cells	1152:1189	These results clearly demonstrate that PAL stimulates the immune response in RAW 264.7 cells through the activation of MAPKs (ERK, p38 and JNK) signaling pathway.
26791584	4	62	theme	Fourier-transform	418:434	arg1	FT-IR					446:450	FT-IR	446:450	FT-IR	446:450	Fourier-transform infrared (FT-IR) analysis indicated that the purified PAL is a complex glycitol, which structurally contains significant amount of hydroxyl groups.
26791584	4	62	theme	Fourier-transform	418:434	arg1	analysis					453:460	Fourier-transform infrared (FT-IR) analysis	418:460	Fourier-transform infrared (FT-IR) analysis	418:460	Fourier-transform infrared (FT-IR) analysis indicated that the purified PAL is a complex glycitol, which structurally contains significant amount of hydroxyl groups.
26791584	9	63	theme	cuspidata	1414:1422	arg1	fruit					1402:1406	the fruit	1398:1406	the fruit of T. cuspidata	1398:1422	To the best of our knowledge, this is the first study to demonstrate the primary structure and immune-stimulating activities of PAL from the fruit of T. cuspidata.
26791584	8	64	theme	RAW	1175:1177	arg1	cells					1185:1189	RAW 264.7 cells	1175:1189	RAW 264.7 cells	1175:1189	These results clearly demonstrate that PAL stimulates the immune response in RAW 264.7 cells through the activation of MAPKs (ERK, p38 and JNK) signaling pathway.
26791584	1	65	attach	isolated	111:118	arg2	PAL					102:104	PAL	102:104	PAL	102:104	A polyhydric alcohol (PAL) was isolated from Taxus cuspidata and its immunostimulatory activities were assessed.
26791584	1	65	attach	isolated	111:118	arg2	alcohol					93:99	A polyhydric alcohol	80:99	A polyhydric alcohol (PAL)	80:105	A polyhydric alcohol (PAL) was isolated from Taxus cuspidata and its immunostimulatory activities were assessed.
26791584	1	65	attach	isolated	111:118	arg1	cuspidata					131:139	Taxus cuspidata	125:139	Taxus cuspidata	125:139	A polyhydric alcohol (PAL) was isolated from Taxus cuspidata and its immunostimulatory activities were assessed.
26791584	8	66	dep	MAPKs	1217:1221	arg1	p38					1229:1231	p38	1229:1231	p38	1229:1231	These results clearly demonstrate that PAL stimulates the immune response in RAW 264.7 cells through the activation of MAPKs (ERK, p38 and JNK) signaling pathway.
26791584	8	66	dep	MAPKs	1217:1221	arg1	ERK					1224:1226	ERK	1224:1226	ERK	1224:1226	These results clearly demonstrate that PAL stimulates the immune response in RAW 264.7 cells through the activation of MAPKs (ERK, p38 and JNK) signaling pathway.
26791584	8	66	dep	MAPKs	1217:1221	arg1	MAPKs					1217:1221	MAPKs	1217:1221	MAPKs (ERK, p38 and JNK) signaling pathway	1217:1258	These results clearly demonstrate that PAL stimulates the immune response in RAW 264.7 cells through the activation of MAPKs (ERK, p38 and JNK) signaling pathway.
26791584	8	66	dep	MAPKs	1217:1221	arg1	JNK					1237:1239	JNK	1237:1239	JNK	1237:1239	These results clearly demonstrate that PAL stimulates the immune response in RAW 264.7 cells through the activation of MAPKs (ERK, p38 and JNK) signaling pathway.
26791584	9	67	theme	first	1303:1307	arg1	this					1291:1294	this	1291:1294	this	1291:1294	To the best of our knowledge, this is the first study to demonstrate the primary structure and immune-stimulating activities of PAL from the fruit of T. cuspidata.
26791584	9	67	theme	first	1303:1307	arg1	study					1309:1313	the first study	1299:1313	the first study to demonstrate the primary structure and immune-stimulating activities of PAL from the fruit of T. cuspidata	1299:1422	To the best of our knowledge, this is the first study to demonstrate the primary structure and immune-stimulating activities of PAL from the fruit of T. cuspidata.
25667930	4	0	theme	bone	936:939	arg1	tissue					941:946	the titanium and bone tissue	919:946	tissue	941:946	High-resolution TEM (HRTEM), energy dispersive X-ray spectroscopy (EDS), and scanning TEM (STEM)/electron energy loss spectroscopic analysis (EELS) were used to investigate the chemical composition and structure at the interface between the titanium and bone tissue.
25667930	5	1	theme	implant	1066:1072	arg1	layer					1053:1057	the oxide layer	1043:1057	the oxide layer of the implant	1043:1072	HRTEM and EDS data showed evidence of crystalline hydroxyapatite and intermixing of bone with the oxide layer of the implant.
25667930	4	2	theme	High-resolution	682:696	arg1	HRTEM					703:707	HRTEM	703:707	HRTEM	703:707	High-resolution TEM (HRTEM), energy dispersive X-ray spectroscopy (EDS), and scanning TEM (STEM)/electron energy loss spectroscopic analysis (EELS) were used to investigate the chemical composition and structure at the interface between the titanium and bone tissue.
25667930	4	2	theme	High-resolution	682:696	arg1	TEM					698:700	High-resolution TEM	682:700	High-resolution TEM (HRTEM)	682:708	High-resolution TEM (HRTEM), energy dispersive X-ray spectroscopy (EDS), and scanning TEM (STEM)/electron energy loss spectroscopic analysis (EELS) were used to investigate the chemical composition and structure at the interface between the titanium and bone tissue.
25667930	3	3	theme	chemical	662:669	arg1	treatment					671:679	any chemical treatment	658:679	any chemical treatment	658:679	To prepare a transmission electron microscopy (TEM) specimen, the natural state was preserved as much as possible by cryofixation and scanning electron microscope/focused ion beam (SEM-FIB) milling without any chemical treatment.
25667930	9	4	theme	carbon	1576:1581	arg1	form					1564:1567	the form	1560:1567	the form of the carbon	1560:1581	The possible existence of the osseohybridization area and the form of the carbon suggest that reconsideration of the standard definition of osseointegration is necessary.
25667930	9	4	theme	carbon	1576:1581	arg1	existence					1515:1523	The possible existence	1502:1523	The possible existence of the osseohybridization area	1502:1554	The possible existence of the osseohybridization area and the form of the carbon suggest that reconsideration of the standard definition of osseointegration is necessary.
25667930	3	5	dep	much	549:552	arg1	as					554:555	as	554:555	as	554:555	To prepare a transmission electron microscopy (TEM) specimen, the natural state was preserved as much as possible by cryofixation and scanning electron microscope/focused ion beam (SEM-FIB) milling without any chemical treatment.
25667930	7	6	theme	energy	1307:1312	arg1	loss					1314:1317	the oxygen energy loss	1296:1317	the oxygen energy loss near edge structures (ELNESs)	1296:1347	In addition, the oxygen energy loss near edge structures (ELNESs) showed a possibility of the presence of CaTiO3.
25667930	9	7	theme	osseohybridization	1532:1549	arg1	area					1551:1554	the osseohybridization area	1528:1554	the osseohybridization area	1528:1554	The possible existence of the osseohybridization area and the form of the carbon suggest that reconsideration of the standard definition of osseointegration is necessary.
25667930	3	8	theme	natural	518:524	arg1	preserved					536:544	preserved	536:544	preserved	536:544	To prepare a transmission electron microscopy (TEM) specimen, the natural state was preserved as much as possible by cryofixation and scanning electron microscope/focused ion beam (SEM-FIB) milling without any chemical treatment.
25667930	3	8	theme	natural	518:524	arg1	state					526:530	the natural state	514:530	the natural state	514:530	To prepare a transmission electron microscopy (TEM) specimen, the natural state was preserved as much as possible by cryofixation and scanning electron microscope/focused ion beam (SEM-FIB) milling without any chemical treatment.
25667930	4	9	theme	dispersive	718:727	arg1	EDS					749:751	EDS	749:751	EDS	749:751	High-resolution TEM (HRTEM), energy dispersive X-ray spectroscopy (EDS), and scanning TEM (STEM)/electron energy loss spectroscopic analysis (EELS) were used to investigate the chemical composition and structure at the interface between the titanium and bone tissue.
25667930	4	9	theme	dispersive	718:727	arg1	spectroscopy					735:746	energy dispersive X-ray spectroscopy	711:746	energy dispersive X-ray spectroscopy (EDS)	711:752	High-resolution TEM (HRTEM), energy dispersive X-ray spectroscopy (EDS), and scanning TEM (STEM)/electron energy loss spectroscopic analysis (EELS) were used to investigate the chemical composition and structure at the interface between the titanium and bone tissue.
25667930	4	10	used	used	835:838	arg2	EELS					824:827	High-resolution TEM (HRTEM), energy dispersive X-ray spectroscopy (EDS), and scanning TEM (STEM)/electron energy loss spectroscopic analysis (EELS)	682:828	High-resolution TEM (HRTEM), energy dispersive X-ray spectroscopy (EDS), and scanning TEM (STEM)/electron energy loss spectroscopic analysis (EELS)	682:828	High-resolution TEM (HRTEM), energy dispersive X-ray spectroscopy (EDS), and scanning TEM (STEM)/electron energy loss spectroscopic analysis (EELS) were used to investigate the chemical composition and structure at the interface between the titanium and bone tissue.
25667930	7	11	theme	CaTiO3	1389:1394	arg1	presence					1377:1384	the presence	1373:1384	the presence of CaTiO3	1373:1394	In addition, the oxygen energy loss near edge structures (ELNESs) showed a possibility of the presence of CaTiO3.
25667930	4	12	theme	spectroscopy	735:746	arg1	EELS					824:827	High-resolution TEM (HRTEM), energy dispersive X-ray spectroscopy (EDS), and scanning TEM (STEM)/electron energy loss spectroscopic analysis (EELS)	682:828	High-resolution TEM (HRTEM), energy dispersive X-ray spectroscopy (EDS), and scanning TEM (STEM)/electron energy loss spectroscopic analysis (EELS)	682:828	High-resolution TEM (HRTEM), energy dispersive X-ray spectroscopy (EDS), and scanning TEM (STEM)/electron energy loss spectroscopic analysis (EELS) were used to investigate the chemical composition and structure at the interface between the titanium and bone tissue.
25667930	2	13	theme	titanium	260:267	arg1	mini-implant					269:280	a sandblasted and acid-etched titanium mini-implant	230:280	a sandblasted and acid-etched titanium mini-implant	230:280	Therefore, in this study, a sandblasted and acid-etched titanium mini-implant was removed from a human patient after 2 months of placement in order to identify the chemical integration mechanism for nanoscale osseointegration of titanium implants.
25667930	3	14	theme	transmission	465:476	arg1	TEM					499:501	TEM	499:501	TEM	499:501	To prepare a transmission electron microscopy (TEM) specimen, the natural state was preserved as much as possible by cryofixation and scanning electron microscope/focused ion beam (SEM-FIB) milling without any chemical treatment.
25667930	3	14	theme	transmission	465:476	arg1	microscopy					487:496	transmission electron microscopy	465:496	a transmission electron microscopy (TEM) specimen	463:511	To prepare a transmission electron microscopy (TEM) specimen, the natural state was preserved as much as possible by cryofixation and scanning electron microscope/focused ion beam (SEM-FIB) milling without any chemical treatment.
25667930	5	15	theme	oxide	1047:1051	arg1	layer					1053:1057	the oxide layer	1043:1057	the oxide layer of the implant	1043:1072	HRTEM and EDS data showed evidence of crystalline hydroxyapatite and intermixing of bone with the oxide layer of the implant.
25667930	4	16	theme	loss	795:798	arg1	EELS					824:827	High-resolution TEM (HRTEM), energy dispersive X-ray spectroscopy (EDS), and scanning TEM (STEM)/electron energy loss spectroscopic analysis (EELS)	682:828	High-resolution TEM (HRTEM), energy dispersive X-ray spectroscopy (EDS), and scanning TEM (STEM)/electron energy loss spectroscopic analysis (EELS)	682:828	High-resolution TEM (HRTEM), energy dispersive X-ray spectroscopy (EDS), and scanning TEM (STEM)/electron energy loss spectroscopic analysis (EELS) were used to investigate the chemical composition and structure at the interface between the titanium and bone tissue.
25667930	2	17	theme	acid-etched	248:258	arg1	mini-implant					269:280	a sandblasted and acid-etched titanium mini-implant	230:280	a sandblasted and acid-etched titanium mini-implant	230:280	Therefore, in this study, a sandblasted and acid-etched titanium mini-implant was removed from a human patient after 2 months of placement in order to identify the chemical integration mechanism for nanoscale osseointegration of titanium implants.
25667930	0	18	theme	titanium	41:48	arg1	surfaces					50:57	human bone and titanium surfaces	26:57	surfaces	50:57	Nanoscale bonding between human bone and titanium surfaces: osseohybridization.
25667930	7	19	theme	presence	1377:1384	arg1	possibility					1358:1368	a possibility	1356:1368	a possibility of the presence of CaTiO3	1356:1394	In addition, the oxygen energy loss near edge structures (ELNESs) showed a possibility of the presence of CaTiO3.
25667930	4	20	theme	energy	788:793	arg1	loss					795:798	scanning TEM (STEM)/electron energy loss	759:798	scanning TEM (STEM)/electron energy loss	759:798	High-resolution TEM (HRTEM), energy dispersive X-ray spectroscopy (EDS), and scanning TEM (STEM)/electron energy loss spectroscopic analysis (EELS) were used to investigate the chemical composition and structure at the interface between the titanium and bone tissue.
25667930	6	21	theme	interesting	1122:1132	arg1	results					1134:1140	particularly interesting results	1109:1140	particularly interesting results	1109:1140	The STEM/EELS experiment provided particularly interesting results: carbon existed in polysaccharides, calcium and phosphorus existed as tricalcium phosphate (TCP), and titanium existed as oxidized titanium.
25667930	2	22	theme	titanium	433:440	arg1	implants					442:449	titanium implants	433:449	titanium implants	433:449	Therefore, in this study, a sandblasted and acid-etched titanium mini-implant was removed from a human patient after 2 months of placement in order to identify the chemical integration mechanism for nanoscale osseointegration of titanium implants.
25667930	8	23	theme	reaction	1486:1493	arg1	layer					1495:1499	a chemical reaction layer	1475:1499	a chemical reaction layer	1475:1499	These STEM/EELS results can be explained by structures either with or without a chemical reaction layer.
25667930	6	24	theme	tricalcium	1212:1221	arg1	phosphate					1223:1231	tricalcium phosphate	1212:1231	tricalcium phosphate (TCP)	1212:1237	The STEM/EELS experiment provided particularly interesting results: carbon existed in polysaccharides, calcium and phosphorus existed as tricalcium phosphate (TCP), and titanium existed as oxidized titanium.
25667930	6	24	theme	tricalcium	1212:1221	arg1	TCP					1234:1236	TCP	1234:1236	TCP	1234:1236	The STEM/EELS experiment provided particularly interesting results: carbon existed in polysaccharides, calcium and phosphorus existed as tricalcium phosphate (TCP), and titanium existed as oxidized titanium.
25667930	4	25	theme	energy	711:716	arg1	EDS					749:751	EDS	749:751	EDS	749:751	High-resolution TEM (HRTEM), energy dispersive X-ray spectroscopy (EDS), and scanning TEM (STEM)/electron energy loss spectroscopic analysis (EELS) were used to investigate the chemical composition and structure at the interface between the titanium and bone tissue.
25667930	4	25	theme	energy	711:716	arg1	spectroscopy					735:746	energy dispersive X-ray spectroscopy	711:746	energy dispersive X-ray spectroscopy (EDS)	711:752	High-resolution TEM (HRTEM), energy dispersive X-ray spectroscopy (EDS), and scanning TEM (STEM)/electron energy loss spectroscopic analysis (EELS) were used to investigate the chemical composition and structure at the interface between the titanium and bone tissue.
25667930	0	26	theme	Nanoscale	0:8	arg1	bonding					10:16	Nanoscale bonding	0:16	Nanoscale bonding between human bone and titanium surfaces	0:57	Nanoscale bonding between human bone and titanium surfaces: osseohybridization.
25667930	8	27	theme	chemical	1477:1484	arg1	layer					1495:1499	a chemical reaction layer	1475:1499	a chemical reaction layer	1475:1499	These STEM/EELS results can be explained by structures either with or without a chemical reaction layer.
25667930	4	28	theme	spectroscopic	800:812	arg1	analysis					814:821	spectroscopic analysis	800:821	spectroscopic analysis	800:821	High-resolution TEM (HRTEM), energy dispersive X-ray spectroscopy (EDS), and scanning TEM (STEM)/electron energy loss spectroscopic analysis (EELS) were used to investigate the chemical composition and structure at the interface between the titanium and bone tissue.
25667930	7	29	theme	oxygen	1300:1305	arg1	loss					1314:1317	the oxygen energy loss	1296:1317	the oxygen energy loss near edge structures (ELNESs)	1296:1347	In addition, the oxygen energy loss near edge structures (ELNESs) showed a possibility of the presence of CaTiO3.
25667930	4	30	dep	TEM	698:700	arg1	analysis					814:821	spectroscopic analysis	800:821	spectroscopic analysis	800:821	High-resolution TEM (HRTEM), energy dispersive X-ray spectroscopy (EDS), and scanning TEM (STEM)/electron energy loss spectroscopic analysis (EELS) were used to investigate the chemical composition and structure at the interface between the titanium and bone tissue.
25667930	1	31	theme	few	171:173	arg1	tests					197:201	a few mechanical detachment tests	169:201	a few mechanical detachment tests	169:201	Until now, the chemical bonding between titanium and bone has been examined only through a few mechanical detachment tests.
25667930	0	32	theme	human	26:30	arg1	bone					32:35	human bone and titanium surfaces	26:57	bone	32:35	Nanoscale bonding between human bone and titanium surfaces: osseohybridization.
25667930	9	33	theme	definition	1628:1637	arg1	reconsideration					1596:1610	reconsideration	1596:1610	reconsideration of the standard definition of osseointegration	1596:1657	The possible existence of the osseohybridization area and the form of the carbon suggest that reconsideration of the standard definition of osseointegration is necessary.
25667930	9	34	theme	area	1551:1554	arg1	form					1564:1567	the form	1560:1567	the form of the carbon	1560:1581	The possible existence of the osseohybridization area and the form of the carbon suggest that reconsideration of the standard definition of osseointegration is necessary.
25667930	9	34	theme	area	1551:1554	arg1	existence					1515:1523	The possible existence	1502:1523	The possible existence of the osseohybridization area	1502:1554	The possible existence of the osseohybridization area and the form of the carbon suggest that reconsideration of the standard definition of osseointegration is necessary.
25667930	0	35	dep	osseohybridization	60:77	arg1	bonding					10:16	Nanoscale bonding	0:16	Nanoscale bonding between human bone and titanium surfaces	0:57	Nanoscale bonding between human bone and titanium surfaces: osseohybridization.
25667930	5	36	theme	EDS	959:961	arg1	data					963:966	EDS data	959:966	EDS data	959:966	HRTEM and EDS data showed evidence of crystalline hydroxyapatite and intermixing of bone with the oxide layer of the implant.
25667930	1	37	theme	mechanical	175:184	arg1	tests					197:201	a few mechanical detachment tests	169:201	a few mechanical detachment tests	169:201	Until now, the chemical bonding between titanium and bone has been examined only through a few mechanical detachment tests.
25667930	2	38	theme	nanoscale	403:411	arg1	osseointegration					413:428	nanoscale osseointegration	403:428	nanoscale osseointegration of titanium implants	403:449	Therefore, in this study, a sandblasted and acid-etched titanium mini-implant was removed from a human patient after 2 months of placement in order to identify the chemical integration mechanism for nanoscale osseointegration of titanium implants.
25667930	4	39	theme	chemical	859:866	arg1	composition					868:878	the chemical composition	855:878	the chemical composition	855:878	High-resolution TEM (HRTEM), energy dispersive X-ray spectroscopy (EDS), and scanning TEM (STEM)/electron energy loss spectroscopic analysis (EELS) were used to investigate the chemical composition and structure at the interface between the titanium and bone tissue.
25667930	5	40	theme	intermixing	1018:1028	arg1	evidence					975:982	evidence	975:982	evidence of crystalline hydroxyapatite and intermixing of bone	975:1036	HRTEM and EDS data showed evidence of crystalline hydroxyapatite and intermixing of bone with the oxide layer of the implant.
25667930	1	41	theme	chemical	95:102	arg1	bonding					104:110	the chemical bonding	91:110	the chemical bonding between titanium and bone	91:136	Until now, the chemical bonding between titanium and bone has been examined only through a few mechanical detachment tests.
25667930	1	42	theme	detachment	186:195	arg1	tests					197:201	a few mechanical detachment tests	169:201	a few mechanical detachment tests	169:201	Until now, the chemical bonding between titanium and bone has been examined only through a few mechanical detachment tests.
25667930	2	43	attach	removed	286:292	arg2	mini-implant					269:280	a sandblasted and acid-etched titanium mini-implant	230:280	a sandblasted and acid-etched titanium mini-implant	230:280	Therefore, in this study, a sandblasted and acid-etched titanium mini-implant was removed from a human patient after 2 months of placement in order to identify the chemical integration mechanism for nanoscale osseointegration of titanium implants.
25667930	2	43	attach	removed	286:292	arg1	patient					307:313	a human patient	299:313	a human patient	299:313	Therefore, in this study, a sandblasted and acid-etched titanium mini-implant was removed from a human patient after 2 months of placement in order to identify the chemical integration mechanism for nanoscale osseointegration of titanium implants.
25667930	9	44	theme	osseointegration	1642:1657	arg1	definition					1628:1637	the standard definition	1615:1637	the standard definition of osseointegration	1615:1657	The possible existence of the osseohybridization area and the form of the carbon suggest that reconsideration of the standard definition of osseointegration is necessary.
25667930	3	45	theme	SEM-FIB	633:639	arg1	milling					642:648	cryofixation and scanning electron microscope/focused ion beam (SEM-FIB) milling	569:648	milling	642:648	To prepare a transmission electron microscopy (TEM) specimen, the natural state was preserved as much as possible by cryofixation and scanning electron microscope/focused ion beam (SEM-FIB) milling without any chemical treatment.
25667930	4	46	theme	STEM	773:776	arg1	loss					795:798	scanning TEM (STEM)/electron energy loss	759:798	scanning TEM (STEM)/electron energy loss	759:798	High-resolution TEM (HRTEM), energy dispersive X-ray spectroscopy (EDS), and scanning TEM (STEM)/electron energy loss spectroscopic analysis (EELS) were used to investigate the chemical composition and structure at the interface between the titanium and bone tissue.
25667930	2	47	theme	integration	377:387	arg1	mechanism					389:397	the chemical integration mechanism	364:397	the chemical integration mechanism for nanoscale osseointegration of titanium implants	364:449	Therefore, in this study, a sandblasted and acid-etched titanium mini-implant was removed from a human patient after 2 months of placement in order to identify the chemical integration mechanism for nanoscale osseointegration of titanium implants.
25667930	3	48	theme	ion	623:625	arg1	milling					642:648	cryofixation and scanning electron microscope/focused ion beam (SEM-FIB) milling	569:648	milling	642:648	To prepare a transmission electron microscopy (TEM) specimen, the natural state was preserved as much as possible by cryofixation and scanning electron microscope/focused ion beam (SEM-FIB) milling without any chemical treatment.
25667930	3	49	theme	cryofixation	569:580	arg1	milling					642:648	cryofixation and scanning electron microscope/focused ion beam (SEM-FIB) milling	569:648	milling	642:648	To prepare a transmission electron microscopy (TEM) specimen, the natural state was preserved as much as possible by cryofixation and scanning electron microscope/focused ion beam (SEM-FIB) milling without any chemical treatment.
25667930	2	50	theme	chemical	368:375	arg1	mechanism					389:397	the chemical integration mechanism	364:397	the chemical integration mechanism for nanoscale osseointegration of titanium implants	364:449	Therefore, in this study, a sandblasted and acid-etched titanium mini-implant was removed from a human patient after 2 months of placement in order to identify the chemical integration mechanism for nanoscale osseointegration of titanium implants.
25667930	8	51	theme	STEM/EELS	1403:1411	arg1	results					1413:1419	These STEM/EELS results	1397:1419	These STEM/EELS results	1397:1419	These STEM/EELS results can be explained by structures either with or without a chemical reaction layer.
25667930	6	52	theme	oxidized	1264:1271	arg1	titanium					1273:1280	oxidized titanium	1264:1280	oxidized titanium	1264:1280	The STEM/EELS experiment provided particularly interesting results: carbon existed in polysaccharides, calcium and phosphorus existed as tricalcium phosphate (TCP), and titanium existed as oxidized titanium.
25667930	3	53	theme	beam	627:630	arg1	milling					642:648	cryofixation and scanning electron microscope/focused ion beam (SEM-FIB) milling	569:648	milling	642:648	To prepare a transmission electron microscopy (TEM) specimen, the natural state was preserved as much as possible by cryofixation and scanning electron microscope/focused ion beam (SEM-FIB) milling without any chemical treatment.
25667930	4	54	theme	/electron	778:786	arg1	loss					795:798	scanning TEM (STEM)/electron energy loss	759:798	scanning TEM (STEM)/electron energy loss	759:798	High-resolution TEM (HRTEM), energy dispersive X-ray spectroscopy (EDS), and scanning TEM (STEM)/electron energy loss spectroscopic analysis (EELS) were used to investigate the chemical composition and structure at the interface between the titanium and bone tissue.
25667930	3	55	theme	electron	478:485	arg1	TEM					499:501	TEM	499:501	TEM	499:501	To prepare a transmission electron microscopy (TEM) specimen, the natural state was preserved as much as possible by cryofixation and scanning electron microscope/focused ion beam (SEM-FIB) milling without any chemical treatment.
25667930	3	55	theme	electron	478:485	arg1	microscopy					487:496	transmission electron microscopy	465:496	a transmission electron microscopy (TEM) specimen	463:511	To prepare a transmission electron microscopy (TEM) specimen, the natural state was preserved as much as possible by cryofixation and scanning electron microscope/focused ion beam (SEM-FIB) milling without any chemical treatment.
25667930	2	56	theme	implants	442:449	arg1	osseointegration					413:428	nanoscale osseointegration	403:428	nanoscale osseointegration of titanium implants	403:449	Therefore, in this study, a sandblasted and acid-etched titanium mini-implant was removed from a human patient after 2 months of placement in order to identify the chemical integration mechanism for nanoscale osseointegration of titanium implants.
25667930	9	57	theme	possible	1506:1513	arg1	existence					1515:1523	The possible existence	1502:1523	The possible existence of the osseohybridization area	1502:1554	The possible existence of the osseohybridization area and the form of the carbon suggest that reconsideration of the standard definition of osseointegration is necessary.
25667930	7	58	theme	edge	1324:1327	arg1	ELNESs					1341:1346	ELNESs	1341:1346	ELNESs	1341:1346	In addition, the oxygen energy loss near edge structures (ELNESs) showed a possibility of the presence of CaTiO3.
25667930	7	58	theme	edge	1324:1327	arg1	structures					1329:1338	edge structures	1324:1338	edge structures (ELNESs)	1324:1347	In addition, the oxygen energy loss near edge structures (ELNESs) showed a possibility of the presence of CaTiO3.
25667930	5	59	theme	crystalline	987:997	arg1	hydroxyapatite					999:1012	crystalline hydroxyapatite	987:1012	crystalline hydroxyapatite	987:1012	HRTEM and EDS data showed evidence of crystalline hydroxyapatite and intermixing of bone with the oxide layer of the implant.
25667930	3	60	theme	microscopy	487:496	arg1	specimen					504:511	a transmission electron microscopy (TEM) specimen	463:511	a transmission electron microscopy (TEM) specimen	463:511	To prepare a transmission electron microscopy (TEM) specimen, the natural state was preserved as much as possible by cryofixation and scanning electron microscope/focused ion beam (SEM-FIB) milling without any chemical treatment.
25667930	4	61	theme	scanning	759:766	arg1	loss					795:798	scanning TEM (STEM)/electron energy loss	759:798	scanning TEM (STEM)/electron energy loss	759:798	High-resolution TEM (HRTEM), energy dispersive X-ray spectroscopy (EDS), and scanning TEM (STEM)/electron energy loss spectroscopic analysis (EELS) were used to investigate the chemical composition and structure at the interface between the titanium and bone tissue.
25667930	3	62	theme	electron	595:602	arg1	milling					642:648	cryofixation and scanning electron microscope/focused ion beam (SEM-FIB) milling	569:648	milling	642:648	To prepare a transmission electron microscopy (TEM) specimen, the natural state was preserved as much as possible by cryofixation and scanning electron microscope/focused ion beam (SEM-FIB) milling without any chemical treatment.
25667930	2	63	theme	sandblasted	232:242	arg1	mini-implant					269:280	a sandblasted and acid-etched titanium mini-implant	230:280	a sandblasted and acid-etched titanium mini-implant	230:280	Therefore, in this study, a sandblasted and acid-etched titanium mini-implant was removed from a human patient after 2 months of placement in order to identify the chemical integration mechanism for nanoscale osseointegration of titanium implants.
25667930	5	64	theme	bone	1033:1036	arg1	hydroxyapatite					999:1012	crystalline hydroxyapatite	987:1012	crystalline hydroxyapatite	987:1012	HRTEM and EDS data showed evidence of crystalline hydroxyapatite and intermixing of bone with the oxide layer of the implant.
25667930	5	64	theme	bone	1033:1036	arg1	intermixing					1018:1028	intermixing	1018:1028	intermixing	1018:1028	HRTEM and EDS data showed evidence of crystalline hydroxyapatite and intermixing of bone with the oxide layer of the implant.
25667930	4	65	theme	X-ray	729:733	arg1	EDS					749:751	EDS	749:751	EDS	749:751	High-resolution TEM (HRTEM), energy dispersive X-ray spectroscopy (EDS), and scanning TEM (STEM)/electron energy loss spectroscopic analysis (EELS) were used to investigate the chemical composition and structure at the interface between the titanium and bone tissue.
25667930	4	65	theme	X-ray	729:733	arg1	spectroscopy					735:746	energy dispersive X-ray spectroscopy	711:746	energy dispersive X-ray spectroscopy (EDS)	711:752	High-resolution TEM (HRTEM), energy dispersive X-ray spectroscopy (EDS), and scanning TEM (STEM)/electron energy loss spectroscopic analysis (EELS) were used to investigate the chemical composition and structure at the interface between the titanium and bone tissue.
25667930	5	66	theme	hydroxyapatite	999:1012	arg1	evidence					975:982	evidence	975:982	evidence of crystalline hydroxyapatite and intermixing of bone	975:1036	HRTEM and EDS data showed evidence of crystalline hydroxyapatite and intermixing of bone with the oxide layer of the implant.
25667930	3	67	theme	microscope/focused	604:621	arg1	milling					642:648	cryofixation and scanning electron microscope/focused ion beam (SEM-FIB) milling	569:648	milling	642:648	To prepare a transmission electron microscopy (TEM) specimen, the natural state was preserved as much as possible by cryofixation and scanning electron microscope/focused ion beam (SEM-FIB) milling without any chemical treatment.
25667930	9	68	theme	standard	1619:1626	arg1	definition					1628:1637	the standard definition	1615:1637	the standard definition of osseointegration	1615:1657	The possible existence of the osseohybridization area and the form of the carbon suggest that reconsideration of the standard definition of osseointegration is necessary.
25667930	2	69	theme	human	301:305	arg1	patient					307:313	a human patient	299:313	a human patient	299:313	Therefore, in this study, a sandblasted and acid-etched titanium mini-implant was removed from a human patient after 2 months of placement in order to identify the chemical integration mechanism for nanoscale osseointegration of titanium implants.
25667930	4	70	theme	titanium	923:930	arg1	tissue					941:946	the titanium and bone tissue	919:946	tissue	941:946	High-resolution TEM (HRTEM), energy dispersive X-ray spectroscopy (EDS), and scanning TEM (STEM)/electron energy loss spectroscopic analysis (EELS) were used to investigate the chemical composition and structure at the interface between the titanium and bone tissue.
25667930	4	71	theme	TEM	768:770	arg1	loss					795:798	scanning TEM (STEM)/electron energy loss	759:798	scanning TEM (STEM)/electron energy loss	759:798	High-resolution TEM (HRTEM), energy dispersive X-ray spectroscopy (EDS), and scanning TEM (STEM)/electron energy loss spectroscopic analysis (EELS) were used to investigate the chemical composition and structure at the interface between the titanium and bone tissue.
25667930	6	72	theme	STEM/EELS	1079:1087	arg1	experiment					1089:1098	The STEM/EELS experiment	1075:1098	The STEM/EELS experiment	1075:1098	The STEM/EELS experiment provided particularly interesting results: carbon existed in polysaccharides, calcium and phosphorus existed as tricalcium phosphate (TCP), and titanium existed as oxidized titanium.
25667930	3	73	theme	scanning	586:593	arg1	milling					642:648	cryofixation and scanning electron microscope/focused ion beam (SEM-FIB) milling	569:648	milling	642:648	To prepare a transmission electron microscopy (TEM) specimen, the natural state was preserved as much as possible by cryofixation and scanning electron microscope/focused ion beam (SEM-FIB) milling without any chemical treatment.
25667930	2	74	theme	placement	333:341	arg1	months					323:328	2 months	321:328	2 months of placement	321:341	Therefore, in this study, a sandblasted and acid-etched titanium mini-implant was removed from a human patient after 2 months of placement in order to identify the chemical integration mechanism for nanoscale osseointegration of titanium implants.
25667930	4	75	theme	TEM	698:700	arg1	EELS					824:827	High-resolution TEM (HRTEM), energy dispersive X-ray spectroscopy (EDS), and scanning TEM (STEM)/electron energy loss spectroscopic analysis (EELS)	682:828	High-resolution TEM (HRTEM), energy dispersive X-ray spectroscopy (EDS), and scanning TEM (STEM)/electron energy loss spectroscopic analysis (EELS)	682:828	High-resolution TEM (HRTEM), energy dispersive X-ray spectroscopy (EDS), and scanning TEM (STEM)/electron energy loss spectroscopic analysis (EELS) were used to investigate the chemical composition and structure at the interface between the titanium and bone tissue.
26651330	12	0	theme	bark	2124:2127	arg1	use					2108:2110	the ethnomedicinal use	2089:2110	the ethnomedicinal use of the stem bark of A. floribunda	2089:2144	CONCLUSION The results obtained from this study support the ethnomedicinal use of the stem bark of A. floribunda and leaves of C. odorata.
26651330	6	1	theme	2,2'-azino-bis-3-	1134:1150	arg1	ABTS					1191:1194	ABTS	1191:1194	ABTS	1191:1194	The in vitro antioxidant activities were evaluated by 1, 1-diphenyl-2-picryl hydrazyl (DPPH) and 2,2'-azino-bis-3-éthylbenzylthiazoline-6-sulphonic acid (ABTS) radical scavenging assays and ferrous ions chelating activity.
26651330	6	1	theme	2,2'-azino-bis-3-	1134:1150	arg1	acid					1185:1188	2,2'-azino-bis-3-éthylbenzylthiazoline-6-sulphonic acid	1134:1188	2,2'-azino-bis-3-éthylbenzylthiazoline-6-sulphonic acid (ABTS)	1134:1195	The in vitro antioxidant activities were evaluated by 1, 1-diphenyl-2-picryl hydrazyl (DPPH) and 2,2'-azino-bis-3-éthylbenzylthiazoline-6-sulphonic acid (ABTS) radical scavenging assays and ferrous ions chelating activity.
26651330	9	2	from	monosaccharides	1611:1625	arg1	fractions					1573:1581	PoS fractions	1569:1581	PoS fractions	1569:1581	In PoS fractions, Ara and Gal were the major monosaccharides found, indicating that arabinogalactans are the primary macromolecules.
26651330	3	3	theme	floribunda	747:756	arg1	bark					763:766	A. floribunda stem bark	744:766	A. floribunda stem bark	744:766	METHODS Three polysaccharide fractions: soluble polysaccharides (PoS), pectins (Pec) and hemicelluloses (Hem) were extracted from A. floribunda stem bark and C. odorata leaves.
26651330	7	4	theme	mononuclear	1327:1337	arg1	PBMCs					1346:1350	PBMCs	1346:1350	PBMCs	1346:1350	Immunomodulatory activities were performed on the peripheral blood mononuclear cells (PBMCs) using proliferation and enzyme linked immunospot (ELISPOT) method to determine the production of an interferon-gamma.
26651330	7	4	theme	mononuclear	1327:1337	arg1	cells					1339:1343	the peripheral blood mononuclear cells	1306:1343	the peripheral blood mononuclear cells (PBMCs)	1306:1351	Immunomodulatory activities were performed on the peripheral blood mononuclear cells (PBMCs) using proliferation and enzyme linked immunospot (ELISPOT) method to determine the production of an interferon-gamma.
26651330	13	5	theme	antioxidative	2239:2251	arg1	activities					2274:2283	the antioxidative and immunomodulative activities	2235:2283	the antioxidative and immunomodulative activities of these fractions	2235:2302	Further research is necessary to have supporting evidence that the antioxidative and immunomodulative activities of these fractions are really connected to the polysaccharides and not polyphenols.
26651330	4	6	theme	total	862:866	arg1	contents					874:881	total sugar contents	862:881	total sugar contents	862:881	These samples were analysed for their proteins, phenolic compounds and total sugar contents.
26651330	6	7	theme	chelating	1240:1248	arg1	activity					1250:1257	chelating activity	1240:1257	chelating activity	1240:1257	The in vitro antioxidant activities were evaluated by 1, 1-diphenyl-2-picryl hydrazyl (DPPH) and 2,2'-azino-bis-3-éthylbenzylthiazoline-6-sulphonic acid (ABTS) radical scavenging assays and ferrous ions chelating activity.
26651330	1	8	theme	Many	176:179	arg1	polysaccharides					187:201	BACKGROUND Many plant polysaccharides	165:201	BACKGROUND Many plant polysaccharides	165:201	BACKGROUND Many plant polysaccharides have shown high antioxidant and immunostimulating properties and can be explored as novel molecules with biological properties that can potentially improve immune function.
26651330	1	8	theme	Many	176:179	arg1	molecules					293:301	novel molecules	287:301	novel molecules with biological properties that can potentially improve immune function	287:373	BACKGROUND Many plant polysaccharides have shown high antioxidant and immunostimulating properties and can be explored as novel molecules with biological properties that can potentially improve immune function.
26651330	6	9	theme	ferrous	1227:1233	arg1	ions					1235:1238	ferrous ions	1227:1238	ferrous ions	1227:1238	The in vitro antioxidant activities were evaluated by 1, 1-diphenyl-2-picryl hydrazyl (DPPH) and 2,2'-azino-bis-3-éthylbenzylthiazoline-6-sulphonic acid (ABTS) radical scavenging assays and ferrous ions chelating activity.
26651330	10	10	theme	Hem	1699:1701	arg1	fractions					1703:1711	Hem fractions	1699:1711	Hem fractions	1699:1711	Hem fractions contained predominantly Xyl and GalA for A. floribunda and Xyl (upto 80 %) for and C. odorata.
26651330	3	11	theme	C.	772:773	arg1	odorata					775:781	C. odorata leaves	772:788	C. odorata leaves	772:788	METHODS Three polysaccharide fractions: soluble polysaccharides (PoS), pectins (Pec) and hemicelluloses (Hem) were extracted from A. floribunda stem bark and C. odorata leaves.
26651330	2	12	theme	immunomodulatory	585:600	arg1	properties					602:611	their antioxidant and immunomodulatory properties	563:611	their antioxidant and immunomodulatory properties	563:611	The objective of this work was to characterize soluble and cell wall polysaccharides isolated from the stem bark of Allanblackia floribunda and Chromolaena odorata leaves and to evaluate their antioxidant and immunomodulatory properties.
26651330	1	13	with	molecules	293:301	arg1	properties					319:328	biological properties	308:328	biological properties that can potentially improve immune function	308:373	BACKGROUND Many plant polysaccharides have shown high antioxidant and immunostimulating properties and can be explored as novel molecules with biological properties that can potentially improve immune function.
26651330	2	14	theme	antioxidant	569:579	arg1	properties					602:611	their antioxidant and immunomodulatory properties	563:611	their antioxidant and immunomodulatory properties	563:611	The objective of this work was to characterize soluble and cell wall polysaccharides isolated from the stem bark of Allanblackia floribunda and Chromolaena odorata leaves and to evaluate their antioxidant and immunomodulatory properties.
26651330	11	15	theme	radical	1896:1902	arg1	scavenging					1904:1913	ABTS radical scavenging	1891:1913	ABTS radical scavenging	1891:1913	A. floribunda Hem fraction and C. odorata PoS fraction showed significant DPPH and ABTS radical scavenging activities and immunostimulatory activity via stimulation of PBMC and production of IFN-γ in a dose-dependent manner.
26651330	3	16	theme	soluble	654:660	arg1	PoS					679:681	PoS	679:681	PoS	679:681	METHODS Three polysaccharide fractions: soluble polysaccharides (PoS), pectins (Pec) and hemicelluloses (Hem) were extracted from A. floribunda stem bark and C. odorata leaves.
26651330	3	16	theme	soluble	654:660	arg1	polysaccharides					662:676	soluble polysaccharides	654:676	soluble polysaccharides (PoS)	654:682	METHODS Three polysaccharide fractions: soluble polysaccharides (PoS), pectins (Pec) and hemicelluloses (Hem) were extracted from A. floribunda stem bark and C. odorata leaves.
26651330	1	17	theme	antioxidant	219:229	arg1	properties					253:262	high antioxidant and immunostimulating properties	214:262	high antioxidant and immunostimulating properties	214:262	BACKGROUND Many plant polysaccharides have shown high antioxidant and immunostimulating properties and can be explored as novel molecules with biological properties that can potentially improve immune function.
26651330	0	18	from	floribunda	81:90	arg1	polysaccharides					47:61	polysaccharides	47:61	polysaccharides from Allanblackia floribunda Oliv stem bark and Chromolaena odorata (L.) King and H.E. Robins	47:155	Antioxidant and immunomodulatory properties of polysaccharides from Allanblackia floribunda Oliv stem bark and Chromolaena odorata (L.) King and H.E. Robins leaves.
26651330	7	19	theme	proliferation	1359:1371	arg1	method					1412:1417	proliferation and enzyme linked immunospot (ELISPOT) method	1359:1417	proliferation and enzyme linked immunospot (ELISPOT) method to determine the production of an interferon-gamma	1359:1468	Immunomodulatory activities were performed on the peripheral blood mononuclear cells (PBMCs) using proliferation and enzyme linked immunospot (ELISPOT) method to determine the production of an interferon-gamma.
26651330	0	20	from	odorata	123:129	arg1	polysaccharides					47:61	polysaccharides	47:61	polysaccharides from Allanblackia floribunda Oliv stem bark and Chromolaena odorata (L.) King and H.E. Robins	47:155	Antioxidant and immunomodulatory properties of polysaccharides from Allanblackia floribunda Oliv stem bark and Chromolaena odorata (L.) King and H.E. Robins leaves.
26651330	11	21	theme	Hem	1822:1824	arg1	fraction					1826:1833	A. floribunda Hem fraction	1808:1833	A. floribunda Hem fraction	1808:1833	A. floribunda Hem fraction and C. odorata PoS fraction showed significant DPPH and ABTS radical scavenging activities and immunostimulatory activity via stimulation of PBMC and production of IFN-γ in a dose-dependent manner.
26651330	5	22	theme	proteins	972:979	arg1	content					981:987	arabinogalactan proteins content	956:987	arabinogalactan proteins content in PoS	956:994	The monosaccharide composition was determined by gas chromatography and arabinogalactan proteins content in PoS was evaluated by rocket electrophoresis.
26651330	2	23	theme	odorata	532:538	arg1	bark					484:487	the stem bark	475:487	the stem bark of Allanblackia floribunda and Chromolaena odorata leaves	475:545	The objective of this work was to characterize soluble and cell wall polysaccharides isolated from the stem bark of Allanblackia floribunda and Chromolaena odorata leaves and to evaluate their antioxidant and immunomodulatory properties.
26651330	7	24	theme	immunospot	1391:1400	arg1	method					1412:1417	proliferation and enzyme linked immunospot (ELISPOT) method	1359:1417	proliferation and enzyme linked immunospot (ELISPOT) method to determine the production of an interferon-gamma	1359:1468	Immunomodulatory activities were performed on the peripheral blood mononuclear cells (PBMCs) using proliferation and enzyme linked immunospot (ELISPOT) method to determine the production of an interferon-gamma.
26651330	12	25	theme	A.	2132:2133	arg1	floribunda					2135:2144	A. floribunda	2132:2144	A. floribunda	2132:2144	CONCLUSION The results obtained from this study support the ethnomedicinal use of the stem bark of A. floribunda and leaves of C. odorata.
26651330	11	26	theme	A.	1808:1809	arg1	fraction					1826:1833	A. floribunda Hem fraction	1808:1833	A. floribunda Hem fraction	1808:1833	A. floribunda Hem fraction and C. odorata PoS fraction showed significant DPPH and ABTS radical scavenging activities and immunostimulatory activity via stimulation of PBMC and production of IFN-γ in a dose-dependent manner.
26651330	7	27	theme	blood	1321:1325	arg1	PBMCs					1346:1350	PBMCs	1346:1350	PBMCs	1346:1350	Immunomodulatory activities were performed on the peripheral blood mononuclear cells (PBMCs) using proliferation and enzyme linked immunospot (ELISPOT) method to determine the production of an interferon-gamma.
26651330	7	27	theme	blood	1321:1325	arg1	cells					1339:1343	the peripheral blood mononuclear cells	1306:1343	the peripheral blood mononuclear cells (PBMCs)	1306:1351	Immunomodulatory activities were performed on the peripheral blood mononuclear cells (PBMCs) using proliferation and enzyme linked immunospot (ELISPOT) method to determine the production of an interferon-gamma.
26651330	6	28	theme	antioxidant	1050:1060	arg1	activities					1062:1071	The in vitro antioxidant activities	1037:1071	The in vitro antioxidant activities	1037:1071	The in vitro antioxidant activities were evaluated by 1, 1-diphenyl-2-picryl hydrazyl (DPPH) and 2,2'-azino-bis-3-éthylbenzylthiazoline-6-sulphonic acid (ABTS) radical scavenging assays and ferrous ions chelating activity.
26651330	11	29	theme	odorata	1842:1848	arg1	fraction					1854:1861	C. odorata PoS fraction	1839:1861	C. odorata PoS fraction	1839:1861	A. floribunda Hem fraction and C. odorata PoS fraction showed significant DPPH and ABTS radical scavenging activities and immunostimulatory activity via stimulation of PBMC and production of IFN-γ in a dose-dependent manner.
26651330	0	30	theme	stem	97:100	arg1	bark					102:105	Oliv stem bark	92:105	Oliv stem bark	92:105	Antioxidant and immunomodulatory properties of polysaccharides from Allanblackia floribunda Oliv stem bark and Chromolaena odorata (L.) King and H.E. Robins leaves.
26651330	5	31	theme	gas	933:935	arg1	chromatography					937:950	gas chromatography	933:950	gas chromatography	933:950	The monosaccharide composition was determined by gas chromatography and arabinogalactan proteins content in PoS was evaluated by rocket electrophoresis.
26651330	6	32	theme	in	1041:1042	arg1	activities					1062:1071	The in vitro antioxidant activities	1037:1071	The in vitro antioxidant activities	1037:1071	The in vitro antioxidant activities were evaluated by 1, 1-diphenyl-2-picryl hydrazyl (DPPH) and 2,2'-azino-bis-3-éthylbenzylthiazoline-6-sulphonic acid (ABTS) radical scavenging assays and ferrous ions chelating activity.
26651330	6	33	dep	in	1041:1042	arg1	vitro					1044:1048	vitro	1044:1048	vitro	1044:1048	The in vitro antioxidant activities were evaluated by 1, 1-diphenyl-2-picryl hydrazyl (DPPH) and 2,2'-azino-bis-3-éthylbenzylthiazoline-6-sulphonic acid (ABTS) radical scavenging assays and ferrous ions chelating activity.
26651330	12	34	dep	CONCLUSION	2033:2042	arg1	support					2081:2087	support	2081:2087	support the ethnomedicinal use of the stem bark of A. floribunda and leaves of C. odorata	2081:2169	CONCLUSION The results obtained from this study support the ethnomedicinal use of the stem bark of A. floribunda and leaves of C. odorata.
26651330	9	35	theme	primary	1675:1681	arg1	macromolecules					1683:1696	the primary macromolecules	1671:1696	the primary macromolecules	1671:1696	In PoS fractions, Ara and Gal were the major monosaccharides found, indicating that arabinogalactans are the primary macromolecules.
26651330	9	35	theme	primary	1675:1681	arg1	arabinogalactans					1650:1665	arabinogalactans	1650:1665	arabinogalactans	1650:1665	In PoS fractions, Ara and Gal were the major monosaccharides found, indicating that arabinogalactans are the primary macromolecules.
26651330	0	36	theme	Oliv	92:95	arg1	bark					102:105	Oliv stem bark	92:105	Oliv stem bark	92:105	Antioxidant and immunomodulatory properties of polysaccharides from Allanblackia floribunda Oliv stem bark and Chromolaena odorata (L.) King and H.E. Robins leaves.
26651330	1	37	theme	novel	287:291	arg1	polysaccharides					187:201	BACKGROUND Many plant polysaccharides	165:201	BACKGROUND Many plant polysaccharides	165:201	BACKGROUND Many plant polysaccharides have shown high antioxidant and immunostimulating properties and can be explored as novel molecules with biological properties that can potentially improve immune function.
26651330	1	37	theme	novel	287:291	arg1	molecules					293:301	novel molecules	287:301	novel molecules with biological properties that can potentially improve immune function	287:373	BACKGROUND Many plant polysaccharides have shown high antioxidant and immunostimulating properties and can be explored as novel molecules with biological properties that can potentially improve immune function.
26651330	2	38	theme	wall	440:443	arg1	polysaccharides					445:459	soluble and cell wall polysaccharides	423:459	polysaccharides	445:459	The objective of this work was to characterize soluble and cell wall polysaccharides isolated from the stem bark of Allanblackia floribunda and Chromolaena odorata leaves and to evaluate their antioxidant and immunomodulatory properties.
26651330	0	39	theme	immunomodulatory	16:31	arg1	properties					33:42	immunomodulatory properties	16:42	immunomodulatory properties	16:42	Antioxidant and immunomodulatory properties of polysaccharides from Allanblackia floribunda Oliv stem bark and Chromolaena odorata (L.) King and H.E. Robins leaves.
26651330	0	40	dep	floribunda	81:90	arg1	King					136:139	King	136:139	King	136:139	Antioxidant and immunomodulatory properties of polysaccharides from Allanblackia floribunda Oliv stem bark and Chromolaena odorata (L.) King and H.E. Robins leaves.
26651330	0	40	dep	floribunda	81:90	arg1	Robins					150:155	Robins	150:155	Robins	150:155	Antioxidant and immunomodulatory properties of polysaccharides from Allanblackia floribunda Oliv stem bark and Chromolaena odorata (L.) King and H.E. Robins leaves.
26651330	0	40	dep	floribunda	81:90	arg1	bark					102:105	Oliv stem bark	92:105	Oliv stem bark	92:105	Antioxidant and immunomodulatory properties of polysaccharides from Allanblackia floribunda Oliv stem bark and Chromolaena odorata (L.) King and H.E. Robins leaves.
26651330	8	41	dep	RESULTS	1471:1477	arg1	characterization					1483:1498	The characterization	1479:1498	RESULTS The characterization of the various fractions	1471:1523	RESULTS The characterization of the various fractions showed varied metabolites in each plant.
26651330	7	42	theme	linked	1384:1389	arg1	immunospot					1391:1400	enzyme linked immunospot	1377:1400	enzyme linked immunospot (ELISPOT)	1377:1410	Immunomodulatory activities were performed on the peripheral blood mononuclear cells (PBMCs) using proliferation and enzyme linked immunospot (ELISPOT) method to determine the production of an interferon-gamma.
26651330	7	42	theme	linked	1384:1389	arg1	ELISPOT					1403:1409	ELISPOT	1403:1409	ELISPOT	1403:1409	Immunomodulatory activities were performed on the peripheral blood mononuclear cells (PBMCs) using proliferation and enzyme linked immunospot (ELISPOT) method to determine the production of an interferon-gamma.
26651330	11	43	theme	immunostimulatory	1930:1946	arg1	activity					1948:1955	immunostimulatory activity	1930:1955	immunostimulatory activity	1930:1955	A. floribunda Hem fraction and C. odorata PoS fraction showed significant DPPH and ABTS radical scavenging activities and immunostimulatory activity via stimulation of PBMC and production of IFN-γ in a dose-dependent manner.
26651330	2	44	dep	floribunda	505:514	arg1	leaves					540:545	leaves	540:545	leaves	540:545	The objective of this work was to characterize soluble and cell wall polysaccharides isolated from the stem bark of Allanblackia floribunda and Chromolaena odorata leaves and to evaluate their antioxidant and immunomodulatory properties.
26651330	11	45	theme	dose-dependent	2010:2023	arg1	manner					2025:2030	a dose-dependent manner	2008:2030	a dose-dependent manner	2008:2030	A. floribunda Hem fraction and C. odorata PoS fraction showed significant DPPH and ABTS radical scavenging activities and immunostimulatory activity via stimulation of PBMC and production of IFN-γ in a dose-dependent manner.
26651330	8	46	theme	fractions	1515:1523	arg1	characterization					1483:1498	The characterization	1479:1498	RESULTS The characterization of the various fractions	1471:1523	RESULTS The characterization of the various fractions showed varied metabolites in each plant.
26651330	7	47	dep	linked	1384:1389	arg1	enzyme					1377:1382	enzyme	1377:1382	enzyme	1377:1382	Immunomodulatory activities were performed on the peripheral blood mononuclear cells (PBMCs) using proliferation and enzyme linked immunospot (ELISPOT) method to determine the production of an interferon-gamma.
26651330	8	48	theme	varied	1532:1537	arg1	metabolites					1539:1549	varied metabolites	1532:1549	varied metabolites in each plant	1532:1563	RESULTS The characterization of the various fractions showed varied metabolites in each plant.
26651330	6	49	theme	éthylbenzylthiazoline-6-sulphonic	1151:1183	arg1	ABTS					1191:1194	ABTS	1191:1194	ABTS	1191:1194	The in vitro antioxidant activities were evaluated by 1, 1-diphenyl-2-picryl hydrazyl (DPPH) and 2,2'-azino-bis-3-éthylbenzylthiazoline-6-sulphonic acid (ABTS) radical scavenging assays and ferrous ions chelating activity.
26651330	6	49	theme	éthylbenzylthiazoline-6-sulphonic	1151:1183	arg1	acid					1185:1188	2,2'-azino-bis-3-éthylbenzylthiazoline-6-sulphonic acid	1134:1188	2,2'-azino-bis-3-éthylbenzylthiazoline-6-sulphonic acid (ABTS)	1134:1195	The in vitro antioxidant activities were evaluated by 1, 1-diphenyl-2-picryl hydrazyl (DPPH) and 2,2'-azino-bis-3-éthylbenzylthiazoline-6-sulphonic acid (ABTS) radical scavenging assays and ferrous ions chelating activity.
26651330	10	50	theme	C.	1796:1797	arg1	odorata					1799:1805	and C. odorata	1792:1805	odorata	1799:1805	Hem fractions contained predominantly Xyl and GalA for A. floribunda and Xyl (upto 80 %) for and C. odorata.
26651330	12	51	theme	stem	2119:2122	arg1	bark					2124:2127	the stem bark	2115:2127	the stem bark of A. floribunda	2115:2144	CONCLUSION The results obtained from this study support the ethnomedicinal use of the stem bark of A. floribunda and leaves of C. odorata.
26651330	7	52	link	linked	1384:1389	arg1	immunospot					1391:1400	enzyme linked immunospot	1377:1400	enzyme linked immunospot (ELISPOT)	1377:1410	Immunomodulatory activities were performed on the peripheral blood mononuclear cells (PBMCs) using proliferation and enzyme linked immunospot (ELISPOT) method to determine the production of an interferon-gamma.
26651330	7	52	link	linked	1384:1389	arg1	ELISPOT					1403:1409	ELISPOT	1403:1409	ELISPOT	1403:1409	Immunomodulatory activities were performed on the peripheral blood mononuclear cells (PBMCs) using proliferation and enzyme linked immunospot (ELISPOT) method to determine the production of an interferon-gamma.
26651330	9	53	theme	major	1605:1609	arg1	Gal					1592:1594	Gal	1592:1594	Gal	1592:1594	In PoS fractions, Ara and Gal were the major monosaccharides found, indicating that arabinogalactans are the primary macromolecules.
26651330	9	53	theme	major	1605:1609	arg1	Ara					1584:1586	Ara	1584:1586	Ara	1584:1586	In PoS fractions, Ara and Gal were the major monosaccharides found, indicating that arabinogalactans are the primary macromolecules.
26651330	9	53	theme	major	1605:1609	arg1	monosaccharides					1611:1625	the major monosaccharides	1601:1625	the major monosaccharides found	1601:1631	In PoS fractions, Ara and Gal were the major monosaccharides found, indicating that arabinogalactans are the primary macromolecules.
26651330	0	54	from	H.E.	145:148	arg1	polysaccharides					47:61	polysaccharides	47:61	polysaccharides from Allanblackia floribunda Oliv stem bark and Chromolaena odorata (L.) King and H.E. Robins	47:155	Antioxidant and immunomodulatory properties of polysaccharides from Allanblackia floribunda Oliv stem bark and Chromolaena odorata (L.) King and H.E. Robins leaves.
26651330	3	55	theme	stem	758:761	arg1	bark					763:766	A. floribunda stem bark	744:766	A. floribunda stem bark	744:766	METHODS Three polysaccharide fractions: soluble polysaccharides (PoS), pectins (Pec) and hemicelluloses (Hem) were extracted from A. floribunda stem bark and C. odorata leaves.
26651330	1	56	theme	plant	181:185	arg1	polysaccharides					187:201	BACKGROUND Many plant polysaccharides	165:201	BACKGROUND Many plant polysaccharides	165:201	BACKGROUND Many plant polysaccharides have shown high antioxidant and immunostimulating properties and can be explored as novel molecules with biological properties that can potentially improve immune function.
26651330	1	56	theme	plant	181:185	arg1	molecules					293:301	novel molecules	287:301	novel molecules with biological properties that can potentially improve immune function	287:373	BACKGROUND Many plant polysaccharides have shown high antioxidant and immunostimulating properties and can be explored as novel molecules with biological properties that can potentially improve immune function.
26651330	1	57	theme	BACKGROUND	165:174	arg1	polysaccharides					187:201	BACKGROUND Many plant polysaccharides	165:201	BACKGROUND Many plant polysaccharides	165:201	BACKGROUND Many plant polysaccharides have shown high antioxidant and immunostimulating properties and can be explored as novel molecules with biological properties that can potentially improve immune function.
26651330	1	57	theme	BACKGROUND	165:174	arg1	molecules					293:301	novel molecules	287:301	novel molecules with biological properties that can potentially improve immune function	287:373	BACKGROUND Many plant polysaccharides have shown high antioxidant and immunostimulating properties and can be explored as novel molecules with biological properties that can potentially improve immune function.
26651330	12	58	theme	ethnomedicinal	2093:2106	arg1	use					2108:2110	the ethnomedicinal use	2089:2110	the ethnomedicinal use of the stem bark of A. floribunda	2089:2144	CONCLUSION The results obtained from this study support the ethnomedicinal use of the stem bark of A. floribunda and leaves of C. odorata.
26651330	3	59	theme	A.	744:745	arg1	bark					763:766	A. floribunda stem bark	744:766	A. floribunda stem bark	744:766	METHODS Three polysaccharide fractions: soluble polysaccharides (PoS), pectins (Pec) and hemicelluloses (Hem) were extracted from A. floribunda stem bark and C. odorata leaves.
26651330	11	60	theme	significant	1870:1880	arg1	DPPH					1882:1885	significant DPPH	1870:1885	significant DPPH	1870:1885	A. floribunda Hem fraction and C. odorata PoS fraction showed significant DPPH and ABTS radical scavenging activities and immunostimulatory activity via stimulation of PBMC and production of IFN-γ in a dose-dependent manner.
26651330	5	61	theme	monosaccharide	888:901	arg1	composition					903:913	The monosaccharide composition	884:913	The monosaccharide composition	884:913	The monosaccharide composition was determined by gas chromatography and arabinogalactan proteins content in PoS was evaluated by rocket electrophoresis.
26651330	4	62	theme	sugar	868:872	arg1	contents					874:881	total sugar contents	862:881	total sugar contents	862:881	These samples were analysed for their proteins, phenolic compounds and total sugar contents.
26651330	11	63	theme	DPPH	1882:1885	arg1	activities					1915:1924	significant DPPH and ABTS radical scavenging activities	1870:1924	significant DPPH and ABTS radical scavenging activities	1870:1924	A. floribunda Hem fraction and C. odorata PoS fraction showed significant DPPH and ABTS radical scavenging activities and immunostimulatory activity via stimulation of PBMC and production of IFN-γ in a dose-dependent manner.
26651330	8	64	from	metabolites	1539:1549	arg1	plant					1559:1563	each plant	1554:1563	each plant	1554:1563	RESULTS The characterization of the various fractions showed varied metabolites in each plant.
26651330	6	65	theme	scavenging	1205:1214	arg1	assays					1216:1221	radical scavenging assays	1197:1221	radical scavenging assays	1197:1221	The in vitro antioxidant activities were evaluated by 1, 1-diphenyl-2-picryl hydrazyl (DPPH) and 2,2'-azino-bis-3-éthylbenzylthiazoline-6-sulphonic acid (ABTS) radical scavenging assays and ferrous ions chelating activity.
26651330	9	66	theme	PoS	1569:1571	arg1	fractions					1573:1581	PoS fractions	1569:1581	PoS fractions	1569:1581	In PoS fractions, Ara and Gal were the major monosaccharides found, indicating that arabinogalactans are the primary macromolecules.
26651330	11	67	theme	scavenging	1904:1913	arg1	activities					1915:1924	significant DPPH and ABTS radical scavenging activities	1870:1924	significant DPPH and ABTS radical scavenging activities	1870:1924	A. floribunda Hem fraction and C. odorata PoS fraction showed significant DPPH and ABTS radical scavenging activities and immunostimulatory activity via stimulation of PBMC and production of IFN-γ in a dose-dependent manner.
26651330	3	68	theme	polysaccharide	628:641	arg1	fractions					643:651	Three polysaccharide fractions	622:651	METHODS Three polysaccharide fractions: soluble polysaccharides (PoS), pectins (Pec) and hemicelluloses (Hem) were extracted from A. floribunda stem bark and C. odorata leaves.	614:789	METHODS Three polysaccharide fractions: soluble polysaccharides (PoS), pectins (Pec) and hemicelluloses (Hem) were extracted from A. floribunda stem bark and C. odorata leaves.
26651330	7	69	theme	interferon-gamma	1453:1468	arg1	production					1436:1445	the production	1432:1445	the production of an interferon-gamma	1432:1468	Immunomodulatory activities were performed on the peripheral blood mononuclear cells (PBMCs) using proliferation and enzyme linked immunospot (ELISPOT) method to determine the production of an interferon-gamma.
26651330	11	70	theme	ABTS	1891:1894	arg1	scavenging					1904:1913	ABTS radical scavenging	1891:1913	ABTS radical scavenging	1891:1913	A. floribunda Hem fraction and C. odorata PoS fraction showed significant DPPH and ABTS radical scavenging activities and immunostimulatory activity via stimulation of PBMC and production of IFN-γ in a dose-dependent manner.
26651330	13	71	theme	Further	2172:2178	arg1	research					2180:2187	Further research	2172:2187	Further research	2172:2187	Further research is necessary to have supporting evidence that the antioxidative and immunomodulative activities of these fractions are really connected to the polysaccharides and not polyphenols.
26651330	5	72	theme	arabinogalactan	956:970	arg1	content					981:987	arabinogalactan proteins content	956:987	arabinogalactan proteins content in PoS	956:994	The monosaccharide composition was determined by gas chromatography and arabinogalactan proteins content in PoS was evaluated by rocket electrophoresis.
26651330	7	73	theme	peripheral	1310:1319	arg1	PBMCs					1346:1350	PBMCs	1346:1350	PBMCs	1346:1350	Immunomodulatory activities were performed on the peripheral blood mononuclear cells (PBMCs) using proliferation and enzyme linked immunospot (ELISPOT) method to determine the production of an interferon-gamma.
26651330	7	73	theme	peripheral	1310:1319	arg1	cells					1339:1343	the peripheral blood mononuclear cells	1306:1343	the peripheral blood mononuclear cells (PBMCs)	1306:1351	Immunomodulatory activities were performed on the peripheral blood mononuclear cells (PBMCs) using proliferation and enzyme linked immunospot (ELISPOT) method to determine the production of an interferon-gamma.
26651330	11	74	theme	floribunda	1811:1820	arg1	fraction					1826:1833	A. floribunda Hem fraction	1808:1833	A. floribunda Hem fraction	1808:1833	A. floribunda Hem fraction and C. odorata PoS fraction showed significant DPPH and ABTS radical scavenging activities and immunostimulatory activity via stimulation of PBMC and production of IFN-γ in a dose-dependent manner.
26651330	1	75	theme	immune	359:364	arg1	function					366:373	immune function	359:373	immune function	359:373	BACKGROUND Many plant polysaccharides have shown high antioxidant and immunostimulating properties and can be explored as novel molecules with biological properties that can potentially improve immune function.
26651330	3	76	dep	METHODS	614:620	arg1	extracted					729:737	extracted	729:737	were extracted from A. floribunda stem bark and C. odorata leaves	724:788	METHODS Three polysaccharide fractions: soluble polysaccharides (PoS), pectins (Pec) and hemicelluloses (Hem) were extracted from A. floribunda stem bark and C. odorata leaves.
26651330	3	76	dep	METHODS	614:620	arg1	fractions					643:651	Three polysaccharide fractions	622:651	METHODS Three polysaccharide fractions: soluble polysaccharides (PoS), pectins (Pec) and hemicelluloses (Hem) were extracted from A. floribunda stem bark and C. odorata leaves.	614:789	METHODS Three polysaccharide fractions: soluble polysaccharides (PoS), pectins (Pec) and hemicelluloses (Hem) were extracted from A. floribunda stem bark and C. odorata leaves.
26651330	2	77	theme	floribunda	505:514	arg1	bark					484:487	the stem bark	475:487	the stem bark of Allanblackia floribunda and Chromolaena odorata leaves	475:545	The objective of this work was to characterize soluble and cell wall polysaccharides isolated from the stem bark of Allanblackia floribunda and Chromolaena odorata leaves and to evaluate their antioxidant and immunomodulatory properties.
26651330	12	78	theme	floribunda	2135:2144	arg1	bark					2124:2127	the stem bark	2115:2127	the stem bark of A. floribunda	2115:2144	CONCLUSION The results obtained from this study support the ethnomedicinal use of the stem bark of A. floribunda and leaves of C. odorata.
26651330	11	79	theme	PoS	1850:1852	arg1	fraction					1854:1861	C. odorata PoS fraction	1839:1861	C. odorata PoS fraction	1839:1861	A. floribunda Hem fraction and C. odorata PoS fraction showed significant DPPH and ABTS radical scavenging activities and immunostimulatory activity via stimulation of PBMC and production of IFN-γ in a dose-dependent manner.
26651330	3	80	dep	odorata	775:781	arg1	leaves					783:788	leaves	783:788	leaves	783:788	METHODS Three polysaccharide fractions: soluble polysaccharides (PoS), pectins (Pec) and hemicelluloses (Hem) were extracted from A. floribunda stem bark and C. odorata leaves.
26651330	11	81	theme	C.	1839:1840	arg1	fraction					1854:1861	C. odorata PoS fraction	1839:1861	C. odorata PoS fraction	1839:1861	A. floribunda Hem fraction and C. odorata PoS fraction showed significant DPPH and ABTS radical scavenging activities and immunostimulatory activity via stimulation of PBMC and production of IFN-γ in a dose-dependent manner.
26651330	7	82	theme	Immunomodulatory	1260:1275	arg1	activities					1277:1286	Immunomodulatory activities	1260:1286	Immunomodulatory activities	1260:1286	Immunomodulatory activities were performed on the peripheral blood mononuclear cells (PBMCs) using proliferation and enzyme linked immunospot (ELISPOT) method to determine the production of an interferon-gamma.
26651330	5	83	theme	rocket	1013:1018	arg1	electrophoresis					1020:1034	rocket electrophoresis	1013:1034	rocket electrophoresis	1013:1034	The monosaccharide composition was determined by gas chromatography and arabinogalactan proteins content in PoS was evaluated by rocket electrophoresis.
26651330	13	84	theme	immunomodulative	2257:2272	arg1	activities					2274:2283	the antioxidative and immunomodulative activities	2235:2283	the antioxidative and immunomodulative activities of these fractions	2235:2302	Further research is necessary to have supporting evidence that the antioxidative and immunomodulative activities of these fractions are really connected to the polysaccharides and not polyphenols.
26651330	10	85	theme	A.	1754:1755	arg1	floribunda					1757:1766	A. floribunda	1754:1766	A. floribunda	1754:1766	Hem fractions contained predominantly Xyl and GalA for A. floribunda and Xyl (upto 80 %) for and C. odorata.
26651330	10	85	theme	A.	1754:1755	arg1	%					1785:1785	upto 80 %	1777:1785	upto 80 %	1777:1785	Hem fractions contained predominantly Xyl and GalA for A. floribunda and Xyl (upto 80 %) for and C. odorata.
26651330	6	86	dep	hydrazyl	1114:1121	arg1	activity					1250:1257	chelating activity	1240:1257	chelating activity	1240:1257	The in vitro antioxidant activities were evaluated by 1, 1-diphenyl-2-picryl hydrazyl (DPPH) and 2,2'-azino-bis-3-éthylbenzylthiazoline-6-sulphonic acid (ABTS) radical scavenging assays and ferrous ions chelating activity.
26651330	6	86	dep	hydrazyl	1114:1121	arg1	assays					1216:1221	radical scavenging assays	1197:1221	radical scavenging assays	1197:1221	The in vitro antioxidant activities were evaluated by 1, 1-diphenyl-2-picryl hydrazyl (DPPH) and 2,2'-azino-bis-3-éthylbenzylthiazoline-6-sulphonic acid (ABTS) radical scavenging assays and ferrous ions chelating activity.
26651330	2	87	theme	work	398:401	arg1	objective					380:388	The objective	376:388	The objective of this work	376:401	The objective of this work was to characterize soluble and cell wall polysaccharides isolated from the stem bark of Allanblackia floribunda and Chromolaena odorata leaves and to evaluate their antioxidant and immunomodulatory properties.
26651330	2	88	attach	isolated	461:468	arg2	polysaccharides					445:459	soluble and cell wall polysaccharides	423:459	polysaccharides	445:459	The objective of this work was to characterize soluble and cell wall polysaccharides isolated from the stem bark of Allanblackia floribunda and Chromolaena odorata leaves and to evaluate their antioxidant and immunomodulatory properties.
26651330	2	88	attach	isolated	461:468	arg1	bark					484:487	the stem bark	475:487	the stem bark of Allanblackia floribunda and Chromolaena odorata leaves	475:545	The objective of this work was to characterize soluble and cell wall polysaccharides isolated from the stem bark of Allanblackia floribunda and Chromolaena odorata leaves and to evaluate their antioxidant and immunomodulatory properties.
26651330	6	89	theme	1-diphenyl-2-picryl	1094:1112	arg1	DPPH					1124:1127	DPPH	1124:1127	DPPH	1124:1127	The in vitro antioxidant activities were evaluated by 1, 1-diphenyl-2-picryl hydrazyl (DPPH) and 2,2'-azino-bis-3-éthylbenzylthiazoline-6-sulphonic acid (ABTS) radical scavenging assays and ferrous ions chelating activity.
26651330	6	89	theme	1-diphenyl-2-picryl	1094:1112	arg1	hydrazyl					1114:1121	1, 1-diphenyl-2-picryl hydrazyl	1091:1121	hydrazyl	1114:1121	The in vitro antioxidant activities were evaluated by 1, 1-diphenyl-2-picryl hydrazyl (DPPH) and 2,2'-azino-bis-3-éthylbenzylthiazoline-6-sulphonic acid (ABTS) radical scavenging assays and ferrous ions chelating activity.
26651330	2	90	theme	cell	435:438	arg1	polysaccharides					445:459	soluble and cell wall polysaccharides	423:459	polysaccharides	445:459	The objective of this work was to characterize soluble and cell wall polysaccharides isolated from the stem bark of Allanblackia floribunda and Chromolaena odorata leaves and to evaluate their antioxidant and immunomodulatory properties.
26651330	0	91	theme	polysaccharides	47:61	arg1	Antioxidant					0:10	Antioxidant	0:10	Antioxidant	0:10	Antioxidant and immunomodulatory properties of polysaccharides from Allanblackia floribunda Oliv stem bark and Chromolaena odorata (L.) King and H.E. Robins leaves.
26651330	1	92	theme	biological	308:317	arg1	properties					319:328	biological properties	308:328	biological properties that can potentially improve immune function	308:373	BACKGROUND Many plant polysaccharides have shown high antioxidant and immunostimulating properties and can be explored as novel molecules with biological properties that can potentially improve immune function.
26651330	1	93	theme	high	214:217	arg1	properties					253:262	high antioxidant and immunostimulating properties	214:262	high antioxidant and immunostimulating properties	214:262	BACKGROUND Many plant polysaccharides have shown high antioxidant and immunostimulating properties and can be explored as novel molecules with biological properties that can potentially improve immune function.
26651330	2	94	theme	soluble	423:429	arg1	polysaccharides					445:459	soluble and cell wall polysaccharides	423:459	polysaccharides	445:459	The objective of this work was to characterize soluble and cell wall polysaccharides isolated from the stem bark of Allanblackia floribunda and Chromolaena odorata leaves and to evaluate their antioxidant and immunomodulatory properties.
26651330	13	95	theme	fractions	2294:2302	arg1	activities					2274:2283	the antioxidative and immunomodulative activities	2235:2283	the antioxidative and immunomodulative activities of these fractions	2235:2302	Further research is necessary to have supporting evidence that the antioxidative and immunomodulative activities of these fractions are really connected to the polysaccharides and not polyphenols.
26651330	6	96	theme	radical	1197:1203	arg1	assays					1216:1221	radical scavenging assays	1197:1221	radical scavenging assays	1197:1221	The in vitro antioxidant activities were evaluated by 1, 1-diphenyl-2-picryl hydrazyl (DPPH) and 2,2'-azino-bis-3-éthylbenzylthiazoline-6-sulphonic acid (ABTS) radical scavenging assays and ferrous ions chelating activity.
26651330	2	97	theme	stem	479:482	arg1	bark					484:487	the stem bark	475:487	the stem bark of Allanblackia floribunda and Chromolaena odorata leaves	475:545	The objective of this work was to characterize soluble and cell wall polysaccharides isolated from the stem bark of Allanblackia floribunda and Chromolaena odorata leaves and to evaluate their antioxidant and immunomodulatory properties.
26651330	4	98	theme	phenolic	839:846	arg1	compounds					848:856	phenolic compounds	839:856	phenolic compounds	839:856	These samples were analysed for their proteins, phenolic compounds and total sugar contents.
26651330	10	99	contain	contained	1713:1721	arg2	Xyl					1737:1739	Xyl	1737:1739	Xyl	1737:1739	Hem fractions contained predominantly Xyl and GalA for A. floribunda and Xyl (upto 80 %) for and C. odorata.
26651330	10	99	contain	contained	1713:1721	arg2	GalA					1745:1748	GalA	1745:1748	GalA	1745:1748	Hem fractions contained predominantly Xyl and GalA for A. floribunda and Xyl (upto 80 %) for and C. odorata.
26651330	10	99	contain	contained	1713:1721	arg1	fractions					1703:1711	Hem fractions	1699:1711	Hem fractions	1699:1711	Hem fractions contained predominantly Xyl and GalA for A. floribunda and Xyl (upto 80 %) for and C. odorata.
26651330	8	100	theme	various	1507:1513	arg1	fractions					1515:1523	the various fractions	1503:1523	the various fractions	1503:1523	RESULTS The characterization of the various fractions showed varied metabolites in each plant.
26651330	5	101	from	content	981:987	arg1	PoS					992:994	PoS	992:994	PoS	992:994	The monosaccharide composition was determined by gas chromatography and arabinogalactan proteins content in PoS was evaluated by rocket electrophoresis.
26651330	1	102	theme	immunostimulating	235:251	arg1	properties					253:262	high antioxidant and immunostimulating properties	214:262	high antioxidant and immunostimulating properties	214:262	BACKGROUND Many plant polysaccharides have shown high antioxidant and immunostimulating properties and can be explored as novel molecules with biological properties that can potentially improve immune function.
26651330	11	103	theme	IFN-γ	1999:2003	arg1	stimulation					1961:1971	stimulation	1961:1971	stimulation of PBMC	1961:1979	A. floribunda Hem fraction and C. odorata PoS fraction showed significant DPPH and ABTS radical scavenging activities and immunostimulatory activity via stimulation of PBMC and production of IFN-γ in a dose-dependent manner.
26651330	11	103	theme	IFN-γ	1999:2003	arg1	production					1985:1994	production	1985:1994	production of IFN-γ	1985:2003	A. floribunda Hem fraction and C. odorata PoS fraction showed significant DPPH and ABTS radical scavenging activities and immunostimulatory activity via stimulation of PBMC and production of IFN-γ in a dose-dependent manner.
26651330	11	104	theme	PBMC	1976:1979	arg1	stimulation					1961:1971	stimulation	1961:1971	stimulation of PBMC	1961:1979	A. floribunda Hem fraction and C. odorata PoS fraction showed significant DPPH and ABTS radical scavenging activities and immunostimulatory activity via stimulation of PBMC and production of IFN-γ in a dose-dependent manner.
26651330	11	104	theme	PBMC	1976:1979	arg1	production					1985:1994	production	1985:1994	production of IFN-γ	1985:2003	A. floribunda Hem fraction and C. odorata PoS fraction showed significant DPPH and ABTS radical scavenging activities and immunostimulatory activity via stimulation of PBMC and production of IFN-γ in a dose-dependent manner.
24755318	4	0	theme	minor	624:628	arg1	changes					644:650	minor compositional changes	624:650	minor compositional changes	624:650	IL treatment caused minor compositional changes but drastically decreased cellulose crystallinity; in particular, when administered after delignification, an X-ray diffractogram similar to that of cellulose II polymorph was observed, suggesting that in the absence of lignin, the cellulose was dissolved in the IL and regenerated in water with a polymorphic transformation.
24755318	4	1	theme	similar	782:788	arg1	diffractogram					768:780	an X-ray diffractogram	759:780	an X-ray diffractogram similar to that of cellulose II polymorph	759:822	IL treatment caused minor compositional changes but drastically decreased cellulose crystallinity; in particular, when administered after delignification, an X-ray diffractogram similar to that of cellulose II polymorph was observed, suggesting that in the absence of lignin, the cellulose was dissolved in the IL and regenerated in water with a polymorphic transformation.
24755318	0	2	theme	1-ethyl-3-methylimidazolium	74:100	arg1	acetate					102:108	the ionic liquid 1-ethyl-3-methylimidazolium acetate	57:108	the ionic liquid 1-ethyl-3-methylimidazolium acetate on enzymatic digestibility of poplar wood	57:150	Synergistic effect of delignification and treatment with the ionic liquid 1-ethyl-3-methylimidazolium acetate on enzymatic digestibility of poplar wood.
24755318	2	3	theme	IL	362:363	arg1	treatment					403:411	chlorite delignification and IL (1-ethyl-3-methylimidazolium acetate) treatment	333:411	chlorite delignification and IL (1-ethyl-3-methylimidazolium acetate) treatment alone or in combination	333:435	Ground biomass was subjected to chlorite delignification and IL (1-ethyl-3-methylimidazolium acetate) treatment alone or in combination.
24755318	4	4	theme	compositional	630:642	arg1	changes					644:650	minor compositional changes	624:650	minor compositional changes	624:650	IL treatment caused minor compositional changes but drastically decreased cellulose crystallinity; in particular, when administered after delignification, an X-ray diffractogram similar to that of cellulose II polymorph was observed, suggesting that in the absence of lignin, the cellulose was dissolved in the IL and regenerated in water with a polymorphic transformation.
24755318	0	5	theme	enzymatic	113:121	arg1	digestibility					123:135	enzymatic digestibility	113:135	enzymatic digestibility of poplar wood	113:150	Synergistic effect of delignification and treatment with the ionic liquid 1-ethyl-3-methylimidazolium acetate on enzymatic digestibility of poplar wood.
24755318	1	6	from	effects	177:183	arg1	digestibility					271:283	the cellulase digestibility	257:283	the cellulase digestibility of poplar wood	257:298	This study examined the effects of removing key recalcitrance factors by ionic liquid (IL) treatment on the cellulase digestibility of poplar wood.
24755318	5	7	theme	delignification-IL	1025:1042	arg1	treatment					1044:1052	the combined delignification-IL treatment	1012:1052	the combined delignification-IL treatment	1012:1052	The structural changes induced by the combined delignification-IL treatment facilitated the enzymatic hydrolysis of cellulose; the biomass could be fully degraded within 72 h by 4 FPU of cellulase per gram glucan, with cellobiose degradation being the rate-limiting step.
24755318	5	8	theme	combined	1016:1023	arg1	treatment					1044:1052	the combined delignification-IL treatment	1012:1052	the combined delignification-IL treatment	1012:1052	The structural changes induced by the combined delignification-IL treatment facilitated the enzymatic hydrolysis of cellulose; the biomass could be fully degraded within 72 h by 4 FPU of cellulase per gram glucan, with cellobiose degradation being the rate-limiting step.
24755318	1	9	theme	cellulase	261:269	arg1	digestibility					271:283	the cellulase digestibility	257:283	the cellulase digestibility of poplar wood	257:298	This study examined the effects of removing key recalcitrance factors by ionic liquid (IL) treatment on the cellulase digestibility of poplar wood.
24755318	4	10	theme	cellulose	801:809	arg1	polymorph					814:822	cellulose II polymorph	801:822	cellulose II polymorph	801:822	IL treatment caused minor compositional changes but drastically decreased cellulose crystallinity; in particular, when administered after delignification, an X-ray diffractogram similar to that of cellulose II polymorph was observed, suggesting that in the absence of lignin, the cellulose was dissolved in the IL and regenerated in water with a polymorphic transformation.
24755318	3	11	theme	compositional	442:454	arg1	features					471:478	The compositional and structural features	438:478	The compositional and structural features of differentially treated biomass samples	438:520	The compositional and structural features of differentially treated biomass samples and their hydrolysis performance at various cellulase loadings were investigated.
24755318	3	12	theme	structural	460:469	arg1	features					471:478	The compositional and structural features	438:478	The compositional and structural features of differentially treated biomass samples	438:520	The compositional and structural features of differentially treated biomass samples and their hydrolysis performance at various cellulase loadings were investigated.
24755318	5	13	theme	cellulase	1165:1173	arg1	FPU					1158:1160	4 FPU	1156:1160	4 FPU of cellulase per gram glucan	1156:1189	The structural changes induced by the combined delignification-IL treatment facilitated the enzymatic hydrolysis of cellulose; the biomass could be fully degraded within 72 h by 4 FPU of cellulase per gram glucan, with cellobiose degradation being the rate-limiting step.
24755318	1	14	theme	key	197:199	arg1	factors					215:221	key recalcitrance factors	197:221	key recalcitrance factors	197:221	This study examined the effects of removing key recalcitrance factors by ionic liquid (IL) treatment on the cellulase digestibility of poplar wood.
24755318	0	15	theme	Synergistic	0:10	arg1	effect					12:17	Synergistic effect	0:17	Synergistic effect of delignification and treatment with the ionic liquid 1-ethyl-3-methylimidazolium acetate on enzymatic digestibility of poplar wood.	0:151	Synergistic effect of delignification and treatment with the ionic liquid 1-ethyl-3-methylimidazolium acetate on enzymatic digestibility of poplar wood.
24755318	5	16	theme	rate-limiting	1230:1242	arg1	step					1244:1247	the rate-limiting step	1226:1247	the rate-limiting step	1226:1247	The structural changes induced by the combined delignification-IL treatment facilitated the enzymatic hydrolysis of cellulose; the biomass could be fully degraded within 72 h by 4 FPU of cellulase per gram glucan, with cellobiose degradation being the rate-limiting step.
24755318	5	16	theme	rate-limiting	1230:1242	arg1	degradation					1208:1218	cellobiose degradation	1197:1218	cellobiose degradation	1197:1218	The structural changes induced by the combined delignification-IL treatment facilitated the enzymatic hydrolysis of cellulose; the biomass could be fully degraded within 72 h by 4 FPU of cellulase per gram glucan, with cellobiose degradation being the rate-limiting step.
24755318	5	17	theme	structural	982:991	arg1	changes					993:999	The structural changes	978:999	The structural changes induced by the combined delignification-IL treatment	978:1052	The structural changes induced by the combined delignification-IL treatment facilitated the enzymatic hydrolysis of cellulose; the biomass could be fully degraded within 72 h by 4 FPU of cellulase per gram glucan, with cellobiose degradation being the rate-limiting step.
24755318	1	18	theme	recalcitrance	201:213	arg1	factors					215:221	key recalcitrance factors	197:221	key recalcitrance factors	197:221	This study examined the effects of removing key recalcitrance factors by ionic liquid (IL) treatment on the cellulase digestibility of poplar wood.
24755318	0	19	theme	delignification	22:36	arg1	effect					12:17	Synergistic effect	0:17	Synergistic effect of delignification and treatment with the ionic liquid 1-ethyl-3-methylimidazolium acetate on enzymatic digestibility of poplar wood.	0:151	Synergistic effect of delignification and treatment with the ionic liquid 1-ethyl-3-methylimidazolium acetate on enzymatic digestibility of poplar wood.
24755318	1	20	theme	poplar	288:293	arg1	wood					295:298	poplar wood	288:298	poplar wood	288:298	This study examined the effects of removing key recalcitrance factors by ionic liquid (IL) treatment on the cellulase digestibility of poplar wood.
24755318	0	21	theme	wood	147:150	arg1	digestibility					123:135	enzymatic digestibility	113:135	enzymatic digestibility of poplar wood	113:150	Synergistic effect of delignification and treatment with the ionic liquid 1-ethyl-3-methylimidazolium acetate on enzymatic digestibility of poplar wood.
24755318	3	22	theme	treated	498:504	arg1	samples					514:520	differentially treated biomass samples	483:520	differentially treated biomass samples	483:520	The compositional and structural features of differentially treated biomass samples and their hydrolysis performance at various cellulase loadings were investigated.
24755318	1	23	theme	wood	295:298	arg1	digestibility					271:283	the cellulase digestibility	257:283	the cellulase digestibility of poplar wood	257:298	This study examined the effects of removing key recalcitrance factors by ionic liquid (IL) treatment on the cellulase digestibility of poplar wood.
24755318	0	24	theme	poplar	140:145	arg1	wood					147:150	poplar wood	140:150	poplar wood	140:150	Synergistic effect of delignification and treatment with the ionic liquid 1-ethyl-3-methylimidazolium acetate on enzymatic digestibility of poplar wood.
24755318	0	25	from	acetate	102:108	arg1	digestibility					123:135	enzymatic digestibility	113:135	enzymatic digestibility of poplar wood	113:150	Synergistic effect of delignification and treatment with the ionic liquid 1-ethyl-3-methylimidazolium acetate on enzymatic digestibility of poplar wood.
24755318	2	26	theme	Ground	301:306	arg1	biomass					308:314	Ground biomass	301:314	Ground biomass	301:314	Ground biomass was subjected to chlorite delignification and IL (1-ethyl-3-methylimidazolium acetate) treatment alone or in combination.
24755318	3	27	theme	biomass	506:512	arg1	samples					514:520	differentially treated biomass samples	483:520	differentially treated biomass samples	483:520	The compositional and structural features of differentially treated biomass samples and their hydrolysis performance at various cellulase loadings were investigated.
24755318	0	28	theme	treatment	42:50	arg1	effect					12:17	Synergistic effect	0:17	Synergistic effect of delignification and treatment with the ionic liquid 1-ethyl-3-methylimidazolium acetate on enzymatic digestibility of poplar wood.	0:151	Synergistic effect of delignification and treatment with the ionic liquid 1-ethyl-3-methylimidazolium acetate on enzymatic digestibility of poplar wood.
24755318	3	29	theme	hydrolysis	532:541	arg1	performance					543:553	their hydrolysis performance	526:553	their hydrolysis performance at various cellulase loadings	526:583	The compositional and structural features of differentially treated biomass samples and their hydrolysis performance at various cellulase loadings were investigated.
24755318	1	30	theme	ionic	226:230	arg1	treatment					244:252	ionic liquid (IL) treatment	226:252	ionic liquid (IL) treatment	226:252	This study examined the effects of removing key recalcitrance factors by ionic liquid (IL) treatment on the cellulase digestibility of poplar wood.
24755318	1	30	theme	ionic	226:230	arg1	IL					240:241	IL	240:241	IL	240:241	This study examined the effects of removing key recalcitrance factors by ionic liquid (IL) treatment on the cellulase digestibility of poplar wood.
24755318	4	31	theme	X-ray	762:766	arg1	diffractogram					768:780	an X-ray diffractogram	759:780	an X-ray diffractogram similar to that of cellulose II polymorph	759:822	IL treatment caused minor compositional changes but drastically decreased cellulose crystallinity; in particular, when administered after delignification, an X-ray diffractogram similar to that of cellulose II polymorph was observed, suggesting that in the absence of lignin, the cellulose was dissolved in the IL and regenerated in water with a polymorphic transformation.
24755318	5	32	theme	cellobiose	1197:1206	arg1	step					1244:1247	the rate-limiting step	1226:1247	the rate-limiting step	1226:1247	The structural changes induced by the combined delignification-IL treatment facilitated the enzymatic hydrolysis of cellulose; the biomass could be fully degraded within 72 h by 4 FPU of cellulase per gram glucan, with cellobiose degradation being the rate-limiting step.
24755318	5	32	theme	cellobiose	1197:1206	arg1	degradation					1208:1218	cellobiose degradation	1197:1218	cellobiose degradation	1197:1218	The structural changes induced by the combined delignification-IL treatment facilitated the enzymatic hydrolysis of cellulose; the biomass could be fully degraded within 72 h by 4 FPU of cellulase per gram glucan, with cellobiose degradation being the rate-limiting step.
24755318	1	33	theme	liquid	232:237	arg1	treatment					244:252	ionic liquid (IL) treatment	226:252	ionic liquid (IL) treatment	226:252	This study examined the effects of removing key recalcitrance factors by ionic liquid (IL) treatment on the cellulase digestibility of poplar wood.
24755318	1	33	theme	liquid	232:237	arg1	IL					240:241	IL	240:241	IL	240:241	This study examined the effects of removing key recalcitrance factors by ionic liquid (IL) treatment on the cellulase digestibility of poplar wood.
24755318	4	34	theme	IL	604:605	arg1	treatment					607:615	IL treatment	604:615	IL treatment	604:615	IL treatment caused minor compositional changes but drastically decreased cellulose crystallinity; in particular, when administered after delignification, an X-ray diffractogram similar to that of cellulose II polymorph was observed, suggesting that in the absence of lignin, the cellulose was dissolved in the IL and regenerated in water with a polymorphic transformation.
24755318	4	35	dep	lignin	872:877	arg1	the					857:859	the	857:859	the	857:859	IL treatment caused minor compositional changes but drastically decreased cellulose crystallinity; in particular, when administered after delignification, an X-ray diffractogram similar to that of cellulose II polymorph was observed, suggesting that in the absence of lignin, the cellulose was dissolved in the IL and regenerated in water with a polymorphic transformation.
24755318	4	35	dep	lignin	872:877	arg1	absence					861:867	absence	861:867	absence	861:867	IL treatment caused minor compositional changes but drastically decreased cellulose crystallinity; in particular, when administered after delignification, an X-ray diffractogram similar to that of cellulose II polymorph was observed, suggesting that in the absence of lignin, the cellulose was dissolved in the IL and regenerated in water with a polymorphic transformation.
24755318	0	36	with	effect	12:17	arg1	acetate					102:108	the ionic liquid 1-ethyl-3-methylimidazolium acetate	57:108	the ionic liquid 1-ethyl-3-methylimidazolium acetate on enzymatic digestibility of poplar wood	57:150	Synergistic effect of delignification and treatment with the ionic liquid 1-ethyl-3-methylimidazolium acetate on enzymatic digestibility of poplar wood.
24755318	2	37	theme	delignification	342:356	arg1	treatment					403:411	chlorite delignification and IL (1-ethyl-3-methylimidazolium acetate) treatment	333:411	chlorite delignification and IL (1-ethyl-3-methylimidazolium acetate) treatment alone or in combination	333:435	Ground biomass was subjected to chlorite delignification and IL (1-ethyl-3-methylimidazolium acetate) treatment alone or in combination.
24755318	4	38	located	observed	828:835	arg1	particular					706:715	particular	706:715	particular	706:715	IL treatment caused minor compositional changes but drastically decreased cellulose crystallinity; in particular, when administered after delignification, an X-ray diffractogram similar to that of cellulose II polymorph was observed, suggesting that in the absence of lignin, the cellulose was dissolved in the IL and regenerated in water with a polymorphic transformation.
24755318	4	38	located	observed	828:835	arg2	diffractogram					768:780	an X-ray diffractogram	759:780	an X-ray diffractogram similar to that of cellulose II polymorph	759:822	IL treatment caused minor compositional changes but drastically decreased cellulose crystallinity; in particular, when administered after delignification, an X-ray diffractogram similar to that of cellulose II polymorph was observed, suggesting that in the absence of lignin, the cellulose was dissolved in the IL and regenerated in water with a polymorphic transformation.
24755318	5	39	theme	enzymatic	1070:1078	arg1	hydrolysis					1080:1089	the enzymatic hydrolysis	1066:1089	the enzymatic hydrolysis of cellulose	1066:1102	The structural changes induced by the combined delignification-IL treatment facilitated the enzymatic hydrolysis of cellulose; the biomass could be fully degraded within 72 h by 4 FPU of cellulase per gram glucan, with cellobiose degradation being the rate-limiting step.
24755318	2	40	theme	chlorite	333:340	arg1	treatment					403:411	chlorite delignification and IL (1-ethyl-3-methylimidazolium acetate) treatment	333:411	chlorite delignification and IL (1-ethyl-3-methylimidazolium acetate) treatment alone or in combination	333:435	Ground biomass was subjected to chlorite delignification and IL (1-ethyl-3-methylimidazolium acetate) treatment alone or in combination.
24755318	4	41	theme	cellulose	678:686	arg1	crystallinity					688:700	cellulose crystallinity	678:700	cellulose crystallinity	678:700	IL treatment caused minor compositional changes but drastically decreased cellulose crystallinity; in particular, when administered after delignification, an X-ray diffractogram similar to that of cellulose II polymorph was observed, suggesting that in the absence of lignin, the cellulose was dissolved in the IL and regenerated in water with a polymorphic transformation.
24755318	4	42	theme	polymorphic	950:960	arg1	transformation					962:975	a polymorphic transformation	948:975	a polymorphic transformation	948:975	IL treatment caused minor compositional changes but drastically decreased cellulose crystallinity; in particular, when administered after delignification, an X-ray diffractogram similar to that of cellulose II polymorph was observed, suggesting that in the absence of lignin, the cellulose was dissolved in the IL and regenerated in water with a polymorphic transformation.
24755318	3	43	theme	cellulase	566:574	arg1	loadings					576:583	various cellulase loadings	558:583	various cellulase loadings	558:583	The compositional and structural features of differentially treated biomass samples and their hydrolysis performance at various cellulase loadings were investigated.
24755318	3	44	theme	samples	514:520	arg1	features					471:478	The compositional and structural features	438:478	The compositional and structural features of differentially treated biomass samples	438:520	The compositional and structural features of differentially treated biomass samples and their hydrolysis performance at various cellulase loadings were investigated.
24755318	3	44	theme	samples	514:520	arg1	performance					543:553	their hydrolysis performance	526:553	their hydrolysis performance at various cellulase loadings	526:583	The compositional and structural features of differentially treated biomass samples and their hydrolysis performance at various cellulase loadings were investigated.
24755318	0	45	theme	liquid	67:72	arg1	acetate					102:108	the ionic liquid 1-ethyl-3-methylimidazolium acetate	57:108	the ionic liquid 1-ethyl-3-methylimidazolium acetate on enzymatic digestibility of poplar wood	57:150	Synergistic effect of delignification and treatment with the ionic liquid 1-ethyl-3-methylimidazolium acetate on enzymatic digestibility of poplar wood.
24755318	3	46	theme	various	558:564	arg1	loadings					576:583	various cellulase loadings	558:583	various cellulase loadings	558:583	The compositional and structural features of differentially treated biomass samples and their hydrolysis performance at various cellulase loadings were investigated.
24755318	0	47	theme	ionic	61:65	arg1	acetate					102:108	the ionic liquid 1-ethyl-3-methylimidazolium acetate	57:108	the ionic liquid 1-ethyl-3-methylimidazolium acetate on enzymatic digestibility of poplar wood	57:150	Synergistic effect of delignification and treatment with the ionic liquid 1-ethyl-3-methylimidazolium acetate on enzymatic digestibility of poplar wood.
24755318	5	48	theme	gram	1179:1182	arg1	glucan					1184:1189	gram glucan	1179:1189	gram glucan	1179:1189	The structural changes induced by the combined delignification-IL treatment facilitated the enzymatic hydrolysis of cellulose; the biomass could be fully degraded within 72 h by 4 FPU of cellulase per gram glucan, with cellobiose degradation being the rate-limiting step.
24755318	5	49	theme	cellulose	1094:1102	arg1	hydrolysis					1080:1089	the enzymatic hydrolysis	1066:1089	the enzymatic hydrolysis of cellulose	1066:1102	The structural changes induced by the combined delignification-IL treatment facilitated the enzymatic hydrolysis of cellulose; the biomass could be fully degraded within 72 h by 4 FPU of cellulase per gram glucan, with cellobiose degradation being the rate-limiting step.
24755318	2	50	theme	1-ethyl-3-methylimidazolium	366:392	arg1	delignification					342:356	delignification	342:356	delignification	342:356	Ground biomass was subjected to chlorite delignification and IL (1-ethyl-3-methylimidazolium acetate) treatment alone or in combination.
24755318	2	50	theme	1-ethyl-3-methylimidazolium	366:392	arg1	acetate					394:400	1-ethyl-3-methylimidazolium acetate	366:400	1-ethyl-3-methylimidazolium acetate	366:400	Ground biomass was subjected to chlorite delignification and IL (1-ethyl-3-methylimidazolium acetate) treatment alone or in combination.
24755318	3	51	from	loadings	576:583	arg1	features					471:478	The compositional and structural features	438:478	The compositional and structural features of differentially treated biomass samples	438:520	The compositional and structural features of differentially treated biomass samples and their hydrolysis performance at various cellulase loadings were investigated.
24755318	3	51	from	loadings	576:583	arg1	performance					543:553	their hydrolysis performance	526:553	their hydrolysis performance at various cellulase loadings	526:583	The compositional and structural features of differentially treated biomass samples and their hydrolysis performance at various cellulase loadings were investigated.
28593759	0	0	theme	Enterotoxigenic	102:116	arg1	K88					135:137	Enterotoxigenic Escherichia coli K88	102:137	Enterotoxigenic Escherichia coli K88	102:137	Enzymatic Synthesis and Purification of Galactosylated Chitosan Oligosaccharides Reducing Adhesion of Enterotoxigenic Escherichia coli K88.
28593759	6	1	contain	had	812:814	arg2	activity					841:848	the highest antiadhesion activity	816:848	the highest antiadhesion activity	816:848	Gal-COS F2 containing the largest oligosaccharides had the highest antiadhesion activity with the minimum inhibitory concentration of 0.22 g/L, followed by F3 and F4.
28593759	6	1	contain	had	812:814	arg1	F2					769:770	Gal-COS F2	761:770	Gal-COS F2 containing the largest oligosaccharides	761:810	Gal-COS F2 containing the largest oligosaccharides had the highest antiadhesion activity with the minimum inhibitory concentration of 0.22 g/L, followed by F3 and F4.
28593759	1	2	theme	causing	265:271	arg1	diarrhea					283:290	ETEC causing childhood diarrhea	260:290	ETEC causing childhood diarrhea	260:290	Enterotoxigenic Escherichia coli (ETEC) K88 causes diarrhea in weaned piglets and represent a suitable model system for ETEC causing childhood diarrhea.
28593759	4	3	theme	cation	588:593	arg1	exchange					595:602	cation exchange	588:602	cation exchange	588:602	Fractions 2-5 of Gal-COS were obtained through cation exchange and size exclusion chromatography.
28593759	7	4	theme	ETEC	985:988	arg1	adhesion					994:1001	ETEC K88 adhesion	985:1001	ETEC K88 adhesion	985:1001	Acetylation of Gal-COS decreased their ability to reduce ETEC K88 adhesion.
28593759	1	5	theme	childhood	273:281	arg1	diarrhea					283:290	ETEC causing childhood diarrhea	260:290	ETEC causing childhood diarrhea	260:290	Enterotoxigenic Escherichia coli (ETEC) K88 causes diarrhea in weaned piglets and represent a suitable model system for ETEC causing childhood diarrhea.
28593759	7	6	theme	K88	990:992	arg1	adhesion					994:1001	ETEC K88 adhesion	985:1001	ETEC K88 adhesion	985:1001	Acetylation of Gal-COS decreased their ability to reduce ETEC K88 adhesion.
28593759	0	7	theme	Escherichia	118:128	arg1	K88					135:137	Enterotoxigenic Escherichia coli K88	102:137	Enterotoxigenic Escherichia coli K88	102:137	Enzymatic Synthesis and Purification of Galactosylated Chitosan Oligosaccharides Reducing Adhesion of Enterotoxigenic Escherichia coli K88.
28593759	9	8	theme	multiple	1272:1279	arg1	pathogens					1281:1289	multiple pathogens	1272:1289	multiple pathogens	1272:1289	Galactosylation of COS produces oligosaccharides which reduce ETEC K88 adhesion; moreover, resulting oligosaccharides match the composition of human milk oligosaccharides, which prevent adhesion of multiple pathogens.
28593759	5	9	theme	chemical	697:704	arg1	acetylation					706:716	chemical acetylation	697:716	chemical acetylation followed by size exclusion chromatography	697:758	Fractions 2-5 of acetylated Gal-COS were obtained through chemical acetylation followed by size exclusion chromatography.
28593759	5	10	dep	Fractions	639:647	arg1	2-5					649:651	2-5	649:651	2-5	649:651	Fractions 2-5 of acetylated Gal-COS were obtained through chemical acetylation followed by size exclusion chromatography.
28593759	2	11	theme	K88	367:369	arg1	adhesion					371:378	ETEC K88 adhesion	362:378	ETEC K88 adhesion to porcine erythrocytes with two bioassays	362:421	This study aimed to evaluate the effects of oligosaccharides against ETEC K88 adhesion to porcine erythrocytes with two bioassays.
28593759	6	12	contain	containing	772:781	arg2	oligosaccharides					795:810	the largest oligosaccharides	783:810	the largest oligosaccharides	783:810	Gal-COS F2 containing the largest oligosaccharides had the highest antiadhesion activity with the minimum inhibitory concentration of 0.22 g/L, followed by F3 and F4.
28593759	6	12	contain	containing	772:781	arg1	F2					769:770	Gal-COS F2	761:770	Gal-COS F2 containing the largest oligosaccharides	761:810	Gal-COS F2 containing the largest oligosaccharides had the highest antiadhesion activity with the minimum inhibitory concentration of 0.22 g/L, followed by F3 and F4.
28593759	6	13	theme	highest	820:826	arg1	activity					841:848	the highest antiadhesion activity	816:848	the highest antiadhesion activity	816:848	Gal-COS F2 containing the largest oligosaccharides had the highest antiadhesion activity with the minimum inhibitory concentration of 0.22 g/L, followed by F3 and F4.
28593759	0	14	theme	K88	135:137	arg1	Adhesion					90:97	Reducing Adhesion	81:97	Reducing Adhesion of Enterotoxigenic Escherichia coli K88	81:137	Enzymatic Synthesis and Purification of Galactosylated Chitosan Oligosaccharides Reducing Adhesion of Enterotoxigenic Escherichia coli K88.
28593759	4	15	theme	Gal-COS	558:564	arg1	Fractions					541:549	Fractions 2-5	541:553	Fractions 2-5 of Gal-COS	541:564	Fractions 2-5 of Gal-COS were obtained through cation exchange and size exclusion chromatography.
28593759	0	16	dep	Escherichia	118:128	arg1	coli					130:133	coli	130:133	coli	130:133	Enzymatic Synthesis and Purification of Galactosylated Chitosan Oligosaccharides Reducing Adhesion of Enterotoxigenic Escherichia coli K88.
28593759	9	17	theme	milk	1223:1226	arg1	oligosaccharides					1228:1243	human milk oligosaccharides	1217:1243	human milk oligosaccharides	1217:1243	Galactosylation of COS produces oligosaccharides which reduce ETEC K88 adhesion; moreover, resulting oligosaccharides match the composition of human milk oligosaccharides, which prevent adhesion of multiple pathogens.
28593759	9	18	theme	COS	1093:1095	arg1	Galactosylation					1074:1088	Galactosylation	1074:1088	Galactosylation of COS	1074:1095	Galactosylation of COS produces oligosaccharides which reduce ETEC K88 adhesion; moreover, resulting oligosaccharides match the composition of human milk oligosaccharides, which prevent adhesion of multiple pathogens.
28593759	5	19	theme	size	730:733	arg1	chromatography					745:758	size exclusion chromatography	730:758	size exclusion chromatography	730:758	Fractions 2-5 of acetylated Gal-COS were obtained through chemical acetylation followed by size exclusion chromatography.
28593759	5	20	theme	acetylated	656:665	arg1	Gal-COS					667:673	acetylated Gal-COS	656:673	acetylated Gal-COS	656:673	Fractions 2-5 of acetylated Gal-COS were obtained through chemical acetylation followed by size exclusion chromatography.
28593759	9	21	theme	oligosaccharides	1228:1243	arg1	composition					1202:1212	the composition	1198:1212	the composition of human milk oligosaccharides, which prevent adhesion of multiple pathogens	1198:1289	Galactosylation of COS produces oligosaccharides which reduce ETEC K88 adhesion; moreover, resulting oligosaccharides match the composition of human milk oligosaccharides, which prevent adhesion of multiple pathogens.
28593759	1	22	theme	weaned	203:208	arg1	piglets					210:216	weaned piglets	203:216	weaned piglets	203:216	Enterotoxigenic Escherichia coli (ETEC) K88 causes diarrhea in weaned piglets and represent a suitable model system for ETEC causing childhood diarrhea.
28593759	0	23	theme	Enzymatic	0:8	arg1	Synthesis					10:18	Enzymatic Synthesis	0:18	Enzymatic Synthesis	0:18	Enzymatic Synthesis and Purification of Galactosylated Chitosan Oligosaccharides Reducing Adhesion of Enterotoxigenic Escherichia coli K88.
28593759	5	24	theme	exclusion	735:743	arg1	chromatography					745:758	size exclusion chromatography	730:758	size exclusion chromatography	730:758	Fractions 2-5 of acetylated Gal-COS were obtained through chemical acetylation followed by size exclusion chromatography.
28593759	5	25	theme	Gal-COS	667:673	arg1	Fractions					639:647	Fractions 2-5	639:651	Fractions 2-5 of acetylated Gal-COS	639:673	Fractions 2-5 of acetylated Gal-COS were obtained through chemical acetylation followed by size exclusion chromatography.
28593759	7	26	theme	Gal-COS	943:949	arg1	Acetylation					928:938	Acetylation	928:938	Acetylation of Gal-COS	928:949	Acetylation of Gal-COS decreased their ability to reduce ETEC K88 adhesion.
28593759	6	27	theme	g/L	900:902	arg1	concentration					878:890	the minimum inhibitory concentration	855:890	the minimum inhibitory concentration of 0.22 g/L	855:902	Gal-COS F2 containing the largest oligosaccharides had the highest antiadhesion activity with the minimum inhibitory concentration of 0.22 g/L, followed by F3 and F4.
28593759	6	28	theme	largest	787:793	arg1	oligosaccharides					795:810	the largest oligosaccharides	783:810	the largest oligosaccharides	783:810	Gal-COS F2 containing the largest oligosaccharides had the highest antiadhesion activity with the minimum inhibitory concentration of 0.22 g/L, followed by F3 and F4.
28593759	8	29	theme	active	1023:1028	arg1	oligosaccharides					1030:1045	active oligosaccharides	1023:1045	active oligosaccharides	1023:1045	The composition of active oligosaccharides was determined with LC-MS.
28593759	0	30	theme	Galactosylated	40:53	arg1	Chitosan					55:62	Galactosylated Chitosan	40:62	Galactosylated Chitosan	40:62	Enzymatic Synthesis and Purification of Galactosylated Chitosan Oligosaccharides Reducing Adhesion of Enterotoxigenic Escherichia coli K88.
28593759	9	31	theme	human	1217:1221	arg1	oligosaccharides					1228:1243	human milk oligosaccharides	1217:1243	human milk oligosaccharides	1217:1243	Galactosylation of COS produces oligosaccharides which reduce ETEC K88 adhesion; moreover, resulting oligosaccharides match the composition of human milk oligosaccharides, which prevent adhesion of multiple pathogens.
28593759	9	32	theme	ETEC	1136:1139	arg1	adhesion					1145:1152	ETEC K88 adhesion	1136:1152	ETEC K88 adhesion	1136:1152	Galactosylation of COS produces oligosaccharides which reduce ETEC K88 adhesion; moreover, resulting oligosaccharides match the composition of human milk oligosaccharides, which prevent adhesion of multiple pathogens.
28593759	1	33	theme	suitable	234:241	arg1	system					249:254	a suitable model system	232:254	a suitable model system for ETEC causing childhood diarrhea	232:290	Enterotoxigenic Escherichia coli (ETEC) K88 causes diarrhea in weaned piglets and represent a suitable model system for ETEC causing childhood diarrhea.
28593759	3	34	theme	Galactosylated	424:437	arg1	chitosan-oligosaccharides					439:463	Galactosylated chitosan-oligosaccharides	424:463	Galactosylated chitosan-oligosaccharides (Gal-COS)	424:473	Galactosylated chitosan-oligosaccharides (Gal-COS) were synthesized through transgalactosylation by β-galactosidase.
28593759	3	34	theme	Galactosylated	424:437	arg1	Gal-COS					466:472	Gal-COS	466:472	Gal-COS	466:472	Galactosylated chitosan-oligosaccharides (Gal-COS) were synthesized through transgalactosylation by β-galactosidase.
28593759	8	35	theme	oligosaccharides	1030:1045	arg1	composition					1008:1018	The composition	1004:1018	The composition of active oligosaccharides	1004:1045	The composition of active oligosaccharides was determined with LC-MS.
28593759	1	36	theme	Enterotoxigenic	140:154	arg1	K88					180:182	Enterotoxigenic Escherichia coli (ETEC) K88	140:182	Enterotoxigenic Escherichia coli (ETEC) K88	140:182	Enterotoxigenic Escherichia coli (ETEC) K88 causes diarrhea in weaned piglets and represent a suitable model system for ETEC causing childhood diarrhea.
28593759	0	37	theme	Chitosan	55:62	arg1	Purification					24:35	Purification	24:35	Purification	24:35	Enzymatic Synthesis and Purification of Galactosylated Chitosan Oligosaccharides Reducing Adhesion of Enterotoxigenic Escherichia coli K88.
28593759	0	37	theme	Chitosan	55:62	arg1	Synthesis					10:18	Enzymatic Synthesis	0:18	Enzymatic Synthesis	0:18	Enzymatic Synthesis and Purification of Galactosylated Chitosan Oligosaccharides Reducing Adhesion of Enterotoxigenic Escherichia coli K88.
28593759	4	38	theme	exclusion	613:621	arg1	chromatography					623:636	size exclusion chromatography	608:636	size exclusion chromatography	608:636	Fractions 2-5 of Gal-COS were obtained through cation exchange and size exclusion chromatography.
28593759	9	39	theme	pathogens	1281:1289	arg1	adhesion					1260:1267	adhesion	1260:1267	adhesion of multiple pathogens	1260:1289	Galactosylation of COS produces oligosaccharides which reduce ETEC K88 adhesion; moreover, resulting oligosaccharides match the composition of human milk oligosaccharides, which prevent adhesion of multiple pathogens.
28593759	2	40	theme	ETEC	362:365	arg1	adhesion					371:378	ETEC K88 adhesion	362:378	ETEC K88 adhesion to porcine erythrocytes with two bioassays	362:421	This study aimed to evaluate the effects of oligosaccharides against ETEC K88 adhesion to porcine erythrocytes with two bioassays.
28593759	4	41	theme	size	608:611	arg1	chromatography					623:636	size exclusion chromatography	608:636	size exclusion chromatography	608:636	Fractions 2-5 of Gal-COS were obtained through cation exchange and size exclusion chromatography.
28593759	2	42	with	adhesion	371:378	arg1	bioassays					413:421	two bioassays	409:421	two bioassays	409:421	This study aimed to evaluate the effects of oligosaccharides against ETEC K88 adhesion to porcine erythrocytes with two bioassays.
28593759	6	43	theme	inhibitory	867:876	arg1	concentration					878:890	the minimum inhibitory concentration	855:890	the minimum inhibitory concentration of 0.22 g/L	855:902	Gal-COS F2 containing the largest oligosaccharides had the highest antiadhesion activity with the minimum inhibitory concentration of 0.22 g/L, followed by F3 and F4.
28593759	1	44	theme	coli	168:171	arg1	K88					180:182	Enterotoxigenic Escherichia coli (ETEC) K88	140:182	Enterotoxigenic Escherichia coli (ETEC) K88	140:182	Enterotoxigenic Escherichia coli (ETEC) K88 causes diarrhea in weaned piglets and represent a suitable model system for ETEC causing childhood diarrhea.
28593759	6	45	theme	Gal-COS	761:767	arg1	F2					769:770	Gal-COS F2	761:770	Gal-COS F2 containing the largest oligosaccharides	761:810	Gal-COS F2 containing the largest oligosaccharides had the highest antiadhesion activity with the minimum inhibitory concentration of 0.22 g/L, followed by F3 and F4.
28593759	1	46	theme	model	243:247	arg1	system					249:254	a suitable model system	232:254	a suitable model system for ETEC causing childhood diarrhea	232:290	Enterotoxigenic Escherichia coli (ETEC) K88 causes diarrhea in weaned piglets and represent a suitable model system for ETEC causing childhood diarrhea.
28593759	6	47	theme	minimum	859:865	arg1	concentration					878:890	the minimum inhibitory concentration	855:890	the minimum inhibitory concentration of 0.22 g/L	855:902	Gal-COS F2 containing the largest oligosaccharides had the highest antiadhesion activity with the minimum inhibitory concentration of 0.22 g/L, followed by F3 and F4.
28593759	2	48	theme	oligosaccharides	337:352	arg1	effects					326:332	the effects	322:332	the effects of oligosaccharides against ETEC K88 adhesion to porcine erythrocytes with two bioassays	322:421	This study aimed to evaluate the effects of oligosaccharides against ETEC K88 adhesion to porcine erythrocytes with two bioassays.
28593759	9	49	theme	resulting	1165:1173	arg1	oligosaccharides					1175:1190	resulting oligosaccharides	1165:1190	resulting oligosaccharides	1165:1190	Galactosylation of COS produces oligosaccharides which reduce ETEC K88 adhesion; moreover, resulting oligosaccharides match the composition of human milk oligosaccharides, which prevent adhesion of multiple pathogens.
28593759	9	50	theme	K88	1141:1143	arg1	adhesion					1145:1152	ETEC K88 adhesion	1136:1152	ETEC K88 adhesion	1136:1152	Galactosylation of COS produces oligosaccharides which reduce ETEC K88 adhesion; moreover, resulting oligosaccharides match the composition of human milk oligosaccharides, which prevent adhesion of multiple pathogens.
28593759	0	51	theme	Reducing	81:88	arg1	Adhesion					90:97	Reducing Adhesion	81:97	Reducing Adhesion of Enterotoxigenic Escherichia coli K88	81:137	Enzymatic Synthesis and Purification of Galactosylated Chitosan Oligosaccharides Reducing Adhesion of Enterotoxigenic Escherichia coli K88.
28593759	1	52	from	diarrhea	191:198	arg1	piglets					210:216	weaned piglets	203:216	weaned piglets	203:216	Enterotoxigenic Escherichia coli (ETEC) K88 causes diarrhea in weaned piglets and represent a suitable model system for ETEC causing childhood diarrhea.
28593759	2	53	theme	porcine	383:389	arg1	erythrocytes					391:402	porcine erythrocytes	383:402	porcine erythrocytes	383:402	This study aimed to evaluate the effects of oligosaccharides against ETEC K88 adhesion to porcine erythrocytes with two bioassays.
28593759	6	54	theme	antiadhesion	828:839	arg1	activity					841:848	the highest antiadhesion activity	816:848	the highest antiadhesion activity	816:848	Gal-COS F2 containing the largest oligosaccharides had the highest antiadhesion activity with the minimum inhibitory concentration of 0.22 g/L, followed by F3 and F4.
28593759	1	55	theme	ETEC	260:263	arg1	diarrhea					283:290	ETEC causing childhood diarrhea	260:290	ETEC causing childhood diarrhea	260:290	Enterotoxigenic Escherichia coli (ETEC) K88 causes diarrhea in weaned piglets and represent a suitable model system for ETEC causing childhood diarrhea.
26986869	7	0	dep	More	1126:1129	arg1	pectin					1131:1136	pectin	1131:1136	pectin	1131:1136	More pectin and less monolignols occurred in radial cell walls than in tangential walls during the dormant stage, but no significant changes were found in other stages, indicating that pectin accumulation facilitates cell wall expansion, with cambium activity transition.
26986869	7	1	theme	cell	1178:1181	arg1	walls					1183:1187	radial cell walls	1171:1187	radial cell walls	1171:1187	More pectin and less monolignols occurred in radial cell walls than in tangential walls during the dormant stage, but no significant changes were found in other stages, indicating that pectin accumulation facilitates cell wall expansion, with cambium activity transition.
26986869	3	2	theme	Cunninghamia	493:504	arg1	fir					488:490	Chinese fir	480:490	Chinese fir (Cunninghamia lanceolata)	480:516	Our analysis of cytological changes in the cambium of Chinese fir (Cunninghamia lanceolata), revealed a significant positive correlation between vascular cambium cell numbers and cambium zone width through the seasonal cycle.
26986869	3	2	theme	Cunninghamia	493:504	arg1	lanceolata					506:515	Cunninghamia lanceolata	493:515	Cunninghamia lanceolata	493:515	Our analysis of cytological changes in the cambium of Chinese fir (Cunninghamia lanceolata), revealed a significant positive correlation between vascular cambium cell numbers and cambium zone width through the seasonal cycle.
26986869	3	3	theme	significant	530:540	arg1	correlation					551:561	a significant positive correlation	528:561	a significant positive correlation between vascular cambium cell numbers and cambium zone width through the seasonal cycle	528:649	Our analysis of cytological changes in the cambium of Chinese fir (Cunninghamia lanceolata), revealed a significant positive correlation between vascular cambium cell numbers and cambium zone width through the seasonal cycle.
26986869	4	4	theme	cell	660:663	arg1	numbers					665:671	Cambium cell numbers	652:671	Cambium cell numbers	652:671	Cambium cell numbers and the cambium cell radial diameter were closely related to xylem formation.
26986869	3	5	from	cambium	469:475	arg1	analysis					430:437	Our analysis	426:437	Our analysis	426:437	Our analysis of cytological changes in the cambium of Chinese fir (Cunninghamia lanceolata), revealed a significant positive correlation between vascular cambium cell numbers and cambium zone width through the seasonal cycle.
26986869	0	6	theme	Seasonal	0:7	arg1	development					9:19	Seasonal development	0:19	Seasonal development of cambial activity in relation to xylem formation in Chinese fir.	0:86	Seasonal development of cambial activity in relation to xylem formation in Chinese fir.
26986869	1	7	theme	secondary	160:168	arg1	phloem					170:175	secondary phloem	160:175	secondary phloem	160:175	The vascular cambium is a lateral meristem which can differentiate into secondary phloem and xylem.
26986869	2	8	theme	considerable	286:297	arg1	attention					299:307	considerable attention	286:307	considerable attention	286:307	The secondary growth of woody plants resulting from vascular cambium activity has been a focus of considerable attention, but the quantitative relationships between cambial activity and secondary xylem formation have been little studied.
26986869	5	9	theme	cambium	894:900	arg1	walls					871:875	cell walls	866:875	cell walls of dormant-stage cambium	866:900	Immuno-labeling showed that de-esterified homogalacturonan and (1-4)-β-d-galactan epitopes were highly abundant in cell walls of dormant-stage cambium, whereas high methylesterified homogalacturonan was strongly labeled in the active stage.
26986869	7	10	theme	More	1126:1129	arg1	monolignols					1147:1157	More pectin and less monolignols	1126:1157	More pectin and less monolignols	1126:1157	More pectin and less monolignols occurred in radial cell walls than in tangential walls during the dormant stage, but no significant changes were found in other stages, indicating that pectin accumulation facilitates cell wall expansion, with cambium activity transition.
26986869	2	11	theme	woody	212:216	arg1	plants					218:223	woody plants	212:223	woody plants resulting from vascular cambium activity	212:264	The secondary growth of woody plants resulting from vascular cambium activity has been a focus of considerable attention, but the quantitative relationships between cambial activity and secondary xylem formation have been little studied.
26986869	5	12	theme	cell	866:869	arg1	walls					871:875	cell walls	866:875	cell walls of dormant-stage cambium	866:900	Immuno-labeling showed that de-esterified homogalacturonan and (1-4)-β-d-galactan epitopes were highly abundant in cell walls of dormant-stage cambium, whereas high methylesterified homogalacturonan was strongly labeled in the active stage.
26986869	3	13	theme	cell	588:591	arg1	numbers					593:599	vascular cambium cell numbers	571:599	vascular cambium cell numbers	571:599	Our analysis of cytological changes in the cambium of Chinese fir (Cunninghamia lanceolata), revealed a significant positive correlation between vascular cambium cell numbers and cambium zone width through the seasonal cycle.
26986869	8	14	theme	wall	1510:1513	arg1	modification					1515:1526	the cell wall modification	1501:1526	the cell wall modification during the active stage	1501:1550	Our quantitative analysis of the relationship between cambial activity and xylem formation, as well as the cell wall modification during the active stage provides useful information about cambial characteristics and xylogenesis.
26986869	2	15	theme	vascular	240:247	arg1	activity					257:264	vascular cambium activity	240:264	vascular cambium activity	240:264	The secondary growth of woody plants resulting from vascular cambium activity has been a focus of considerable attention, but the quantitative relationships between cambial activity and secondary xylem formation have been little studied.
26986869	3	16	dep	significant	530:540	arg1	positive					542:549	positive	542:549	positive	542:549	Our analysis of cytological changes in the cambium of Chinese fir (Cunninghamia lanceolata), revealed a significant positive correlation between vascular cambium cell numbers and cambium zone width through the seasonal cycle.
26986869	7	17	theme	wall	1348:1351	arg1	expansion					1353:1361	cell wall expansion	1343:1361	cell wall expansion	1343:1361	More pectin and less monolignols occurred in radial cell walls than in tangential walls during the dormant stage, but no significant changes were found in other stages, indicating that pectin accumulation facilitates cell wall expansion, with cambium activity transition.
26986869	4	18	theme	cambium	681:687	arg1	diameter					701:708	the cambium cell radial diameter	677:708	the cambium cell radial diameter	677:708	Cambium cell numbers and the cambium cell radial diameter were closely related to xylem formation.
26986869	4	18	theme	cambium	681:687	arg1	related					723:729	related	723:729	related	723:729	Cambium cell numbers and the cambium cell radial diameter were closely related to xylem formation.
26986869	0	19	theme	xylem	56:60	arg1	formation					62:70	xylem formation	56:70	xylem formation in Chinese fir	56:85	Seasonal development of cambial activity in relation to xylem formation in Chinese fir.
26986869	3	20	theme	zone	613:616	arg1	width					618:622	cambium zone width	605:622	cambium zone width through the seasonal cycle	605:649	Our analysis of cytological changes in the cambium of Chinese fir (Cunninghamia lanceolata), revealed a significant positive correlation between vascular cambium cell numbers and cambium zone width through the seasonal cycle.
26986869	5	21	from	walls	871:875	arg1	abundant					854:861	abundant	854:861	abundant	854:861	Immuno-labeling showed that de-esterified homogalacturonan and (1-4)-β-d-galactan epitopes were highly abundant in cell walls of dormant-stage cambium, whereas high methylesterified homogalacturonan was strongly labeled in the active stage.
26986869	6	22	theme	chemical	1047:1054	arg1	composition					1056:1066	the chemical composition	1043:1066	the chemical composition of cell walls	1043:1080	Raman spectroscopy detected significant changes in the chemical composition of cell walls during the active-dormant stage transition.
26986869	8	23	theme	active	1539:1544	arg1	stage					1546:1550	the active stage	1535:1550	the active stage	1535:1550	Our quantitative analysis of the relationship between cambial activity and xylem formation, as well as the cell wall modification during the active stage provides useful information about cambial characteristics and xylogenesis.
26986869	4	24	theme	radial	694:699	arg1	diameter					701:708	the cambium cell radial diameter	677:708	the cambium cell radial diameter	677:708	Cambium cell numbers and the cambium cell radial diameter were closely related to xylem formation.
26986869	4	24	theme	radial	694:699	arg1	related					723:729	related	723:729	related	723:729	Cambium cell numbers and the cambium cell radial diameter were closely related to xylem formation.
26986869	5	25	theme	de-esterified	779:791	arg1	homogalacturonan					793:808	de-esterified homogalacturonan	779:808	de-esterified homogalacturonan	779:808	Immuno-labeling showed that de-esterified homogalacturonan and (1-4)-β-d-galactan epitopes were highly abundant in cell walls of dormant-stage cambium, whereas high methylesterified homogalacturonan was strongly labeled in the active stage.
26986869	5	26	theme	methylesterified	916:931	arg1	homogalacturonan					933:948	high methylesterified homogalacturonan	911:948	high methylesterified homogalacturonan	911:948	Immuno-labeling showed that de-esterified homogalacturonan and (1-4)-β-d-galactan epitopes were highly abundant in cell walls of dormant-stage cambium, whereas high methylesterified homogalacturonan was strongly labeled in the active stage.
26986869	6	27	theme	significant	1020:1030	arg1	changes					1032:1038	significant changes	1020:1038	significant changes in the chemical composition of cell walls	1020:1080	Raman spectroscopy detected significant changes in the chemical composition of cell walls during the active-dormant stage transition.
26986869	6	28	from	changes	1032:1038	arg1	composition					1056:1066	the chemical composition	1043:1066	the chemical composition of cell walls	1043:1080	Raman spectroscopy detected significant changes in the chemical composition of cell walls during the active-dormant stage transition.
26986869	8	29	theme	quantitative	1402:1413	arg1	analysis					1415:1422	Our quantitative analysis	1398:1422	Our quantitative analysis of the relationship between cambial activity and xylem formation, as well as the cell wall modification during the active stage	1398:1550	Our quantitative analysis of the relationship between cambial activity and xylem formation, as well as the cell wall modification during the active stage provides useful information about cambial characteristics and xylogenesis.
26986869	7	30	theme	activity	1377:1384	arg1	transition					1386:1395	cambium activity transition	1369:1395	cambium activity transition	1369:1395	More pectin and less monolignols occurred in radial cell walls than in tangential walls during the dormant stage, but no significant changes were found in other stages, indicating that pectin accumulation facilitates cell wall expansion, with cambium activity transition.
26986869	8	31	theme	cambial	1586:1592	arg1	characteristics					1594:1608	cambial characteristics	1586:1608	cambial characteristics	1586:1608	Our quantitative analysis of the relationship between cambial activity and xylem formation, as well as the cell wall modification during the active stage provides useful information about cambial characteristics and xylogenesis.
26986869	4	32	theme	xylem	734:738	arg1	formation					740:748	xylem formation	734:748	xylem formation	734:748	Cambium cell numbers and the cambium cell radial diameter were closely related to xylem formation.
26986869	2	33	theme	secondary	374:382	arg1	formation					390:398	secondary xylem formation	374:398	secondary xylem formation	374:398	The secondary growth of woody plants resulting from vascular cambium activity has been a focus of considerable attention, but the quantitative relationships between cambial activity and secondary xylem formation have been little studied.
26986869	5	34	theme	-β-d-galactan	819:831	arg1	epitopes					833:840	de-esterified homogalacturonan and (1-4)-β-d-galactan epitopes	779:840	de-esterified homogalacturonan and (1-4)-β-d-galactan epitopes	779:840	Immuno-labeling showed that de-esterified homogalacturonan and (1-4)-β-d-galactan epitopes were highly abundant in cell walls of dormant-stage cambium, whereas high methylesterified homogalacturonan was strongly labeled in the active stage.
26986869	6	35	theme	stage	1108:1112	arg1	transition					1114:1123	the active-dormant stage transition	1089:1123	the active-dormant stage transition	1089:1123	Raman spectroscopy detected significant changes in the chemical composition of cell walls during the active-dormant stage transition.
26986869	8	36	theme	relationship	1431:1442	arg1	analysis					1415:1422	Our quantitative analysis	1398:1422	Our quantitative analysis of the relationship between cambial activity and xylem formation, as well as the cell wall modification during the active stage	1398:1550	Our quantitative analysis of the relationship between cambial activity and xylem formation, as well as the cell wall modification during the active stage provides useful information about cambial characteristics and xylogenesis.
26986869	1	37	theme	vascular	92:99	arg1	cambium					101:107	The vascular cambium	88:107	The vascular cambium	88:107	The vascular cambium is a lateral meristem which can differentiate into secondary phloem and xylem.
26986869	1	37	theme	vascular	92:99	arg1	meristem					122:129	a lateral meristem	112:129	a lateral meristem which can differentiate into secondary phloem and xylem	112:185	The vascular cambium is a lateral meristem which can differentiate into secondary phloem and xylem.
26986869	7	38	theme	dormant	1225:1231	arg1	stage					1233:1237	the dormant stage	1221:1237	the dormant stage	1221:1237	More pectin and less monolignols occurred in radial cell walls than in tangential walls during the dormant stage, but no significant changes were found in other stages, indicating that pectin accumulation facilitates cell wall expansion, with cambium activity transition.
26986869	8	39	theme	cambial	1452:1458	arg1	activity					1460:1467	cambial activity	1452:1467	cambial activity	1452:1467	Our quantitative analysis of the relationship between cambial activity and xylem formation, as well as the cell wall modification during the active stage provides useful information about cambial characteristics and xylogenesis.
26986869	3	40	theme	cytological	442:452	arg1	changes					454:460	cytological changes	442:460	cytological changes in the cambium of Chinese fir (Cunninghamia lanceolata)	442:516	Our analysis of cytological changes in the cambium of Chinese fir (Cunninghamia lanceolata), revealed a significant positive correlation between vascular cambium cell numbers and cambium zone width through the seasonal cycle.
26986869	0	41	theme	activity	32:39	arg1	development					9:19	Seasonal development	0:19	Seasonal development of cambial activity in relation to xylem formation in Chinese fir.	0:86	Seasonal development of cambial activity in relation to xylem formation in Chinese fir.
26986869	7	42	theme	tangential	1197:1206	arg1	walls					1208:1212	tangential walls	1197:1212	tangential walls	1197:1212	More pectin and less monolignols occurred in radial cell walls than in tangential walls during the dormant stage, but no significant changes were found in other stages, indicating that pectin accumulation facilitates cell wall expansion, with cambium activity transition.
26986869	7	43	theme	cell	1343:1346	arg1	expansion					1353:1361	cell wall expansion	1343:1361	cell wall expansion	1343:1361	More pectin and less monolignols occurred in radial cell walls than in tangential walls during the dormant stage, but no significant changes were found in other stages, indicating that pectin accumulation facilitates cell wall expansion, with cambium activity transition.
26986869	3	44	theme	seasonal	636:643	arg1	cycle					645:649	the seasonal cycle	632:649	the seasonal cycle	632:649	Our analysis of cytological changes in the cambium of Chinese fir (Cunninghamia lanceolata), revealed a significant positive correlation between vascular cambium cell numbers and cambium zone width through the seasonal cycle.
26986869	3	45	from	changes	454:460	arg1	cambium					469:475	the cambium	465:475	the cambium of Chinese fir (Cunninghamia lanceolata)	465:516	Our analysis of cytological changes in the cambium of Chinese fir (Cunninghamia lanceolata), revealed a significant positive correlation between vascular cambium cell numbers and cambium zone width through the seasonal cycle.
26986869	3	46	theme	Chinese	480:486	arg1	fir					488:490	Chinese fir	480:490	Chinese fir (Cunninghamia lanceolata)	480:516	Our analysis of cytological changes in the cambium of Chinese fir (Cunninghamia lanceolata), revealed a significant positive correlation between vascular cambium cell numbers and cambium zone width through the seasonal cycle.
26986869	3	46	theme	Chinese	480:486	arg1	lanceolata					506:515	Cunninghamia lanceolata	493:515	Cunninghamia lanceolata	493:515	Our analysis of cytological changes in the cambium of Chinese fir (Cunninghamia lanceolata), revealed a significant positive correlation between vascular cambium cell numbers and cambium zone width through the seasonal cycle.
26986869	6	47	theme	walls	1076:1080	arg1	composition					1056:1066	the chemical composition	1043:1066	the chemical composition of cell walls	1043:1080	Raman spectroscopy detected significant changes in the chemical composition of cell walls during the active-dormant stage transition.
26986869	7	48	theme	significant	1247:1257	arg1	changes					1259:1265	no significant changes	1244:1265	no significant changes	1244:1265	More pectin and less monolignols occurred in radial cell walls than in tangential walls during the dormant stage, but no significant changes were found in other stages, indicating that pectin accumulation facilitates cell wall expansion, with cambium activity transition.
26986869	7	49	theme	radial	1171:1176	arg1	walls					1183:1187	radial cell walls	1171:1187	radial cell walls	1171:1187	More pectin and less monolignols occurred in radial cell walls than in tangential walls during the dormant stage, but no significant changes were found in other stages, indicating that pectin accumulation facilitates cell wall expansion, with cambium activity transition.
26986869	7	50	theme	other	1281:1285	arg1	stages					1287:1292	other stages	1281:1292	other stages	1281:1292	More pectin and less monolignols occurred in radial cell walls than in tangential walls during the dormant stage, but no significant changes were found in other stages, indicating that pectin accumulation facilitates cell wall expansion, with cambium activity transition.
26986869	2	51	theme	quantitative	318:329	arg1	relationships					331:343	the quantitative relationships	314:343	the quantitative relationships between cambial activity and secondary xylem formation	314:398	The secondary growth of woody plants resulting from vascular cambium activity has been a focus of considerable attention, but the quantitative relationships between cambial activity and secondary xylem formation have been little studied.
26986869	7	52	theme	less	1142:1145	arg1	monolignols					1147:1157	More pectin and less monolignols	1126:1157	More pectin and less monolignols	1126:1157	More pectin and less monolignols occurred in radial cell walls than in tangential walls during the dormant stage, but no significant changes were found in other stages, indicating that pectin accumulation facilitates cell wall expansion, with cambium activity transition.
26986869	4	53	theme	Cambium	652:658	arg1	numbers					665:671	Cambium cell numbers	652:671	Cambium cell numbers	652:671	Cambium cell numbers and the cambium cell radial diameter were closely related to xylem formation.
26986869	2	54	theme	attention	299:307	arg1	growth					202:207	The secondary growth	188:207	The secondary growth of woody plants resulting from vascular cambium activity	188:264	The secondary growth of woody plants resulting from vascular cambium activity has been a focus of considerable attention, but the quantitative relationships between cambial activity and secondary xylem formation have been little studied.
26986869	2	54	theme	attention	299:307	arg1	focus					277:281	a focus	275:281	a focus of considerable attention	275:307	The secondary growth of woody plants resulting from vascular cambium activity has been a focus of considerable attention, but the quantitative relationships between cambial activity and secondary xylem formation have been little studied.
26986869	5	55	theme	dormant-stage	880:892	arg1	cambium					894:900	dormant-stage cambium	880:900	dormant-stage cambium	880:900	Immuno-labeling showed that de-esterified homogalacturonan and (1-4)-β-d-galactan epitopes were highly abundant in cell walls of dormant-stage cambium, whereas high methylesterified homogalacturonan was strongly labeled in the active stage.
26986869	8	56	theme	useful	1561:1566	arg1	information					1568:1578	useful information	1561:1578	useful information about cambial characteristics and xylogenesis	1561:1624	Our quantitative analysis of the relationship between cambial activity and xylem formation, as well as the cell wall modification during the active stage provides useful information about cambial characteristics and xylogenesis.
26986869	3	57	theme	vascular	571:578	arg1	numbers					593:599	vascular cambium cell numbers	571:599	vascular cambium cell numbers	571:599	Our analysis of cytological changes in the cambium of Chinese fir (Cunninghamia lanceolata), revealed a significant positive correlation between vascular cambium cell numbers and cambium zone width through the seasonal cycle.
26986869	6	58	theme	Raman	992:996	arg1	spectroscopy					998:1009	Raman spectroscopy	992:1009	Raman spectroscopy	992:1009	Raman spectroscopy detected significant changes in the chemical composition of cell walls during the active-dormant stage transition.
26986869	3	59	theme	fir	488:490	arg1	cambium					469:475	the cambium	465:475	the cambium of Chinese fir (Cunninghamia lanceolata)	465:516	Our analysis of cytological changes in the cambium of Chinese fir (Cunninghamia lanceolata), revealed a significant positive correlation between vascular cambium cell numbers and cambium zone width through the seasonal cycle.
26986869	2	60	theme	plants	218:223	arg1	growth					202:207	The secondary growth	188:207	The secondary growth of woody plants resulting from vascular cambium activity	188:264	The secondary growth of woody plants resulting from vascular cambium activity has been a focus of considerable attention, but the quantitative relationships between cambial activity and secondary xylem formation have been little studied.
26986869	2	60	theme	plants	218:223	arg1	focus					277:281	a focus	275:281	a focus of considerable attention	275:307	The secondary growth of woody plants resulting from vascular cambium activity has been a focus of considerable attention, but the quantitative relationships between cambial activity and secondary xylem formation have been little studied.
26986869	8	61	theme	cell	1505:1508	arg1	modification					1515:1526	the cell wall modification	1501:1526	the cell wall modification during the active stage	1501:1550	Our quantitative analysis of the relationship between cambial activity and xylem formation, as well as the cell wall modification during the active stage provides useful information about cambial characteristics and xylogenesis.
26986869	1	62	theme	lateral	114:120	arg1	cambium					101:107	The vascular cambium	88:107	The vascular cambium	88:107	The vascular cambium is a lateral meristem which can differentiate into secondary phloem and xylem.
26986869	1	62	theme	lateral	114:120	arg1	meristem					122:129	a lateral meristem	112:129	a lateral meristem which can differentiate into secondary phloem and xylem	112:185	The vascular cambium is a lateral meristem which can differentiate into secondary phloem and xylem.
26986869	3	63	theme	cambium	580:586	arg1	numbers					593:599	vascular cambium cell numbers	571:599	vascular cambium cell numbers	571:599	Our analysis of cytological changes in the cambium of Chinese fir (Cunninghamia lanceolata), revealed a significant positive correlation between vascular cambium cell numbers and cambium zone width through the seasonal cycle.
26986869	8	64	theme	modification	1515:1526	arg1	analysis					1415:1422	Our quantitative analysis	1398:1422	Our quantitative analysis of the relationship between cambial activity and xylem formation, as well as the cell wall modification during the active stage	1398:1550	Our quantitative analysis of the relationship between cambial activity and xylem formation, as well as the cell wall modification during the active stage provides useful information about cambial characteristics and xylogenesis.
26986869	2	65	theme	cambium	249:255	arg1	activity					257:264	vascular cambium activity	240:264	vascular cambium activity	240:264	The secondary growth of woody plants resulting from vascular cambium activity has been a focus of considerable attention, but the quantitative relationships between cambial activity and secondary xylem formation have been little studied.
26986869	5	66	from	abundant	854:861	arg1	walls					871:875	cell walls	866:875	cell walls of dormant-stage cambium	866:900	Immuno-labeling showed that de-esterified homogalacturonan and (1-4)-β-d-galactan epitopes were highly abundant in cell walls of dormant-stage cambium, whereas high methylesterified homogalacturonan was strongly labeled in the active stage.
26986869	6	67	theme	cell	1071:1074	arg1	walls					1076:1080	cell walls	1071:1080	cell walls	1071:1080	Raman spectroscopy detected significant changes in the chemical composition of cell walls during the active-dormant stage transition.
26986869	3	68	theme	cambium	605:611	arg1	width					618:622	cambium zone width	605:622	cambium zone width through the seasonal cycle	605:649	Our analysis of cytological changes in the cambium of Chinese fir (Cunninghamia lanceolata), revealed a significant positive correlation between vascular cambium cell numbers and cambium zone width through the seasonal cycle.
26986869	0	69	theme	Chinese	75:81	arg1	fir					83:85	Chinese fir	75:85	Chinese fir	75:85	Seasonal development of cambial activity in relation to xylem formation in Chinese fir.
26986869	5	70	theme	high	911:914	arg1	homogalacturonan					933:948	high methylesterified homogalacturonan	911:948	high methylesterified homogalacturonan	911:948	Immuno-labeling showed that de-esterified homogalacturonan and (1-4)-β-d-galactan epitopes were highly abundant in cell walls of dormant-stage cambium, whereas high methylesterified homogalacturonan was strongly labeled in the active stage.
26986869	0	71	from	development	9:19	arg1	relation					44:51	relation	44:51	relation to xylem formation in Chinese fir	44:85	Seasonal development of cambial activity in relation to xylem formation in Chinese fir.
26986869	4	72	theme	cell	689:692	arg1	diameter					701:708	the cambium cell radial diameter	677:708	the cambium cell radial diameter	677:708	Cambium cell numbers and the cambium cell radial diameter were closely related to xylem formation.
26986869	4	72	theme	cell	689:692	arg1	related					723:729	related	723:729	related	723:729	Cambium cell numbers and the cambium cell radial diameter were closely related to xylem formation.
26986869	7	73	theme	cambium	1369:1375	arg1	transition					1386:1395	cambium activity transition	1369:1395	cambium activity transition	1369:1395	More pectin and less monolignols occurred in radial cell walls than in tangential walls during the dormant stage, but no significant changes were found in other stages, indicating that pectin accumulation facilitates cell wall expansion, with cambium activity transition.
26986869	2	74	theme	xylem	384:388	arg1	formation					390:398	secondary xylem formation	374:398	secondary xylem formation	374:398	The secondary growth of woody plants resulting from vascular cambium activity has been a focus of considerable attention, but the quantitative relationships between cambial activity and secondary xylem formation have been little studied.
26986869	0	75	theme	cambial	24:30	arg1	activity					32:39	cambial activity	24:39	cambial activity	24:39	Seasonal development of cambial activity in relation to xylem formation in Chinese fir.
26986869	5	76	theme	active	978:983	arg1	stage					985:989	the active stage	974:989	the active stage	974:989	Immuno-labeling showed that de-esterified homogalacturonan and (1-4)-β-d-galactan epitopes were highly abundant in cell walls of dormant-stage cambium, whereas high methylesterified homogalacturonan was strongly labeled in the active stage.
26986869	6	77	theme	active-dormant	1093:1106	arg1	transition					1114:1123	the active-dormant stage transition	1089:1123	the active-dormant stage transition	1089:1123	Raman spectroscopy detected significant changes in the chemical composition of cell walls during the active-dormant stage transition.
26986869	5	78	theme	homogalacturonan	793:808	arg1	epitopes					833:840	de-esterified homogalacturonan and (1-4)-β-d-galactan epitopes	779:840	de-esterified homogalacturonan and (1-4)-β-d-galactan epitopes	779:840	Immuno-labeling showed that de-esterified homogalacturonan and (1-4)-β-d-galactan epitopes were highly abundant in cell walls of dormant-stage cambium, whereas high methylesterified homogalacturonan was strongly labeled in the active stage.
26986869	3	79	theme	changes	454:460	arg1	analysis					430:437	Our analysis	426:437	Our analysis	426:437	Our analysis of cytological changes in the cambium of Chinese fir (Cunninghamia lanceolata), revealed a significant positive correlation between vascular cambium cell numbers and cambium zone width through the seasonal cycle.
26986869	7	80	theme	pectin	1311:1316	arg1	accumulation					1318:1329	pectin accumulation	1311:1329	pectin accumulation	1311:1329	More pectin and less monolignols occurred in radial cell walls than in tangential walls during the dormant stage, but no significant changes were found in other stages, indicating that pectin accumulation facilitates cell wall expansion, with cambium activity transition.
26986869	2	81	theme	secondary	192:200	arg1	growth					202:207	The secondary growth	188:207	The secondary growth of woody plants resulting from vascular cambium activity	188:264	The secondary growth of woody plants resulting from vascular cambium activity has been a focus of considerable attention, but the quantitative relationships between cambial activity and secondary xylem formation have been little studied.
26986869	2	81	theme	secondary	192:200	arg1	focus					277:281	a focus	275:281	a focus of considerable attention	275:307	The secondary growth of woody plants resulting from vascular cambium activity has been a focus of considerable attention, but the quantitative relationships between cambial activity and secondary xylem formation have been little studied.
26986869	0	82	from	formation	62:70	arg1	fir					83:85	Chinese fir	75:85	Chinese fir	75:85	Seasonal development of cambial activity in relation to xylem formation in Chinese fir.
26986869	8	83	theme	xylem	1473:1477	arg1	formation					1479:1487	xylem formation	1473:1487	xylem formation	1473:1487	Our quantitative analysis of the relationship between cambial activity and xylem formation, as well as the cell wall modification during the active stage provides useful information about cambial characteristics and xylogenesis.
26986869	7	84	located	found	1272:1276	arg1	stages					1287:1292	other stages	1281:1292	other stages	1281:1292	More pectin and less monolignols occurred in radial cell walls than in tangential walls during the dormant stage, but no significant changes were found in other stages, indicating that pectin accumulation facilitates cell wall expansion, with cambium activity transition.
26986869	7	84	located	found	1272:1276	arg2	changes					1259:1265	no significant changes	1244:1265	no significant changes	1244:1265	More pectin and less monolignols occurred in radial cell walls than in tangential walls during the dormant stage, but no significant changes were found in other stages, indicating that pectin accumulation facilitates cell wall expansion, with cambium activity transition.
26986869	3	85	from	analysis	430:437	arg1	cambium					469:475	the cambium	465:475	the cambium of Chinese fir (Cunninghamia lanceolata)	465:516	Our analysis of cytological changes in the cambium of Chinese fir (Cunninghamia lanceolata), revealed a significant positive correlation between vascular cambium cell numbers and cambium zone width through the seasonal cycle.
26986869	2	86	theme	cambial	353:359	arg1	activity					361:368	cambial activity	353:368	cambial activity	353:368	The secondary growth of woody plants resulting from vascular cambium activity has been a focus of considerable attention, but the quantitative relationships between cambial activity and secondary xylem formation have been little studied.
27107126	6	0	theme	constructed	932:942	arg1	derivatives					956:966	The constructed EPS-reduced derivatives	928:966	The constructed EPS-reduced derivatives	928:966	The constructed EPS-reduced derivatives were analyzed for the abundance and sugar compositions of their EPS, revealing cps2-like gene clusters in SF2A35B and Lp90 responsible for major EPS production.
27107126	7	1	theme	host	1265:1268	arg1	epithelium					1270:1279	the host epithelium	1261:1279	the host epithelium in the intestinal tract, including bacterial surface properties as well as survival	1261:1363	Moreover, these mutant strains were tested for phenotypic characteristics that are of relevance for their capacity to interact with the host epithelium in the intestinal tract, including bacterial surface properties as well as survival under the stress conditions encountered in the gastrointestinal tract (acid and bile stress).
27107126	2	2	located	present	284:290	arg2	Polysaccharides					251:265	Polysaccharides	251:265	Polysaccharides	251:265	Polysaccharides are ubiquitously present on the cell surface of lactobacilli and are considered to contribute to the species- and strain-specific probiotic effects that are typically observed.
27107126	2	2	located	present	284:290	arg1	surface					304:310	the cell surface	295:310	the cell surface of lactobacilli	295:326	Polysaccharides are ubiquitously present on the cell surface of lactobacilli and are considered to contribute to the species- and strain-specific probiotic effects that are typically observed.
27107126	7	3	from	epithelium	1270:1279	arg1	tract					1299:1303	the intestinal tract	1284:1303	the intestinal tract	1284:1303	Moreover, these mutant strains were tested for phenotypic characteristics that are of relevance for their capacity to interact with the host epithelium in the intestinal tract, including bacterial surface properties as well as survival under the stress conditions encountered in the gastrointestinal tract (acid and bile stress).
27107126	10	4	theme	host	2154:2157	arg1	cells					2159:2163	human host cells	2148:2163	human host cells	2148:2163	IMPORTANCE This study evaluates the role of extracellular polysaccharides that are produced by different strains of Lactobacillus plantarum in the determination of the cell surface properties of these bacteria and their capacity to interact with their environment, including their signaling to human host cells.
27107126	12	5	theme	health-promoting	2456:2471	arg1	organisms					2483:2491	health-promoting probiotic organisms	2456:2491	health-promoting probiotic organisms	2456:2491	In the context of the use of lactobacilli as health-promoting probiotic organisms, this study exemplifies the importance of strain specificity.
27107126	10	6	from	strains	1959:1965	arg1	determination					2001:2013	the determination	1997:2013	the determination of the cell surface properties of these bacteria	1997:2062	IMPORTANCE This study evaluates the role of extracellular polysaccharides that are produced by different strains of Lactobacillus plantarum in the determination of the cell surface properties of these bacteria and their capacity to interact with their environment, including their signaling to human host cells.
27107126	10	6	from	strains	1959:1965	arg1	capacity					2074:2081	their capacity to interact with their environment	2068:2116	their capacity to interact with their environment	2068:2116	IMPORTANCE This study evaluates the role of extracellular polysaccharides that are produced by different strains of Lactobacillus plantarum in the determination of the cell surface properties of these bacteria and their capacity to interact with their environment, including their signaling to human host cells.
27107126	11	7	theme	environmental	2356:2368	arg1	interactions					2370:2381	the environmental interactions	2352:2381	the environmental interactions of these bacterial strains	2352:2408	The results clearly show that the consequences of removal of these polysaccharides are very strain specific, illustrating the diverse and unpredictable roles of these polysaccharides in the environmental interactions of these bacterial strains.
27107126	4	8	theme	plantarum	685:693	arg1	strains					695:701	these L. plantarum strains	676:701	these L. plantarum strains	676:701	In this work, we set out to identify the genes involved in EPS production in these L. plantarum strains and to demonstrate their role in EPS production by gene deletion analysis.
27107126	8	9	theme	EPS	1669:1671	arg1	impacts					1658:1664	strain-specific impacts	1642:1664	strain-specific impacts of EPS on the immunomodulatory properties	1642:1706	In addition, the Toll-like receptor 2 (TLR2) signaling and immunomodulatory capacities of the EPS-negative derivatives and their respective wild-type strains were compared, revealing strain-specific impacts of EPS on the immunomodulatory properties.
27107126	2	10	theme	lactobacilli	315:326	arg1	surface					304:310	the cell surface	295:310	the cell surface of lactobacilli	295:326	Polysaccharides are ubiquitously present on the cell surface of lactobacilli and are considered to contribute to the species- and strain-specific probiotic effects that are typically observed.
27107126	1	11	theme	probiotic	205:213	arg1	supplements					238:248	probiotic, health-promoting food supplements	205:248	probiotic, health-promoting food supplements	205:248	UNLABELLED Lactobacilli are found in diverse environments and are widely applied as probiotic, health-promoting food supplements.
27107126	1	11	theme	probiotic	205:213	arg1	Lactobacilli					132:143	UNLABELLED Lactobacilli	121:143	UNLABELLED Lactobacilli	121:143	UNLABELLED Lactobacilli are found in diverse environments and are widely applied as probiotic, health-promoting food supplements.
27107126	7	12	dep	tract	1429:1433	arg1	acid					1436:1439	acid	1436:1439	acid	1436:1439	Moreover, these mutant strains were tested for phenotypic characteristics that are of relevance for their capacity to interact with the host epithelium in the intestinal tract, including bacterial surface properties as well as survival under the stress conditions encountered in the gastrointestinal tract (acid and bile stress).
27107126	7	12	dep	tract	1429:1433	arg1	stress					1450:1455	bile stress	1445:1455	bile stress	1445:1455	Moreover, these mutant strains were tested for phenotypic characteristics that are of relevance for their capacity to interact with the host epithelium in the intestinal tract, including bacterial surface properties as well as survival under the stress conditions encountered in the gastrointestinal tract (acid and bile stress).
27107126	11	13	theme	bacterial	2392:2400	arg1	strains					2402:2408	these bacterial strains	2386:2408	these bacterial strains	2386:2408	The results clearly show that the consequences of removal of these polysaccharides are very strain specific, illustrating the diverse and unpredictable roles of these polysaccharides in the environmental interactions of these bacterial strains.
27107126	10	14	dep	IMPORTANCE	1854:1863	arg1	evaluates					1876:1884	evaluates	1876:1884	evaluates the role of extracellular polysaccharides that are produced by different strains of Lactobacillus plantarum in the determination of the cell surface properties of these bacteria and their capacity to interact with their environment, including their signaling to human host cells	1876:2163	IMPORTANCE This study evaluates the role of extracellular polysaccharides that are produced by different strains of Lactobacillus plantarum in the determination of the cell surface properties of these bacteria and their capacity to interact with their environment, including their signaling to human host cells.
27107126	2	15	dep	species-	368:375	arg1	effects					407:413	probiotic effects	397:413	probiotic effects	397:413	Polysaccharides are ubiquitously present on the cell surface of lactobacilli and are considered to contribute to the species- and strain-specific probiotic effects that are typically observed.
27107126	2	15	dep	species-	368:375	arg1	the					364:366	the	364:366	the	364:366	Polysaccharides are ubiquitously present on the cell surface of lactobacilli and are considered to contribute to the species- and strain-specific probiotic effects that are typically observed.
27107126	10	16	theme	surface	2027:2033	arg1	properties					2035:2044	the cell surface properties	2018:2044	the cell surface properties of these bacteria	2018:2062	IMPORTANCE This study evaluates the role of extracellular polysaccharides that are produced by different strains of Lactobacillus plantarum in the determination of the cell surface properties of these bacteria and their capacity to interact with their environment, including their signaling to human host cells.
27107126	4	17	from	production	662:671	arg1	strains					695:701	these L. plantarum strains	676:701	these L. plantarum strains	676:701	In this work, we set out to identify the genes involved in EPS production in these L. plantarum strains and to demonstrate their role in EPS production by gene deletion analysis.
27107126	10	18	from	determination	2001:2013	arg1	signaling					2135:2143	their signaling	2129:2143	their signaling to human host cells	2129:2163	IMPORTANCE This study evaluates the role of extracellular polysaccharides that are produced by different strains of Lactobacillus plantarum in the determination of the cell surface properties of these bacteria and their capacity to interact with their environment, including their signaling to human host cells.
27107126	10	18	from	determination	2001:2013	arg1	strains					1959:1965	different strains	1949:1965	different strains	1949:1965	IMPORTANCE This study evaluates the role of extracellular polysaccharides that are produced by different strains of Lactobacillus plantarum in the determination of the cell surface properties of these bacteria and their capacity to interact with their environment, including their signaling to human host cells.
27107126	7	19	theme	gastrointestinal	1412:1427	arg1	tract					1429:1433	the gastrointestinal tract	1408:1433	the gastrointestinal tract (acid and bile stress)	1408:1456	Moreover, these mutant strains were tested for phenotypic characteristics that are of relevance for their capacity to interact with the host epithelium in the intestinal tract, including bacterial surface properties as well as survival under the stress conditions encountered in the gastrointestinal tract (acid and bile stress).
27107126	5	20	theme	reduced	870:876	arg1	levels					878:883	reduced levels	870:883	reduced levels of EPS	870:890	A model L. plantarum strain, WCFS1, and its previously constructed derivative that produced reduced levels of EPS were included as reference strains.
27107126	9	21	theme	EPS	1772:1774	arg1	importance					1758:1767	the importance	1754:1767	the importance of EPS in L. plantarum strains	1754:1798	Taken together, these experiments illustrate the importance of EPS in L. plantarum strains as a strain-specific determinant in host interaction.
27107126	9	21	theme	EPS	1772:1774	arg1	determinant					1821:1831	a strain-specific determinant	1803:1831	a strain-specific determinant in host interaction	1803:1851	Taken together, these experiments illustrate the importance of EPS in L. plantarum strains as a strain-specific determinant in host interaction.
27107126	4	22	theme	EPS	736:738	arg1	production					740:749	EPS production	736:749	EPS production by gene deletion analysis	736:775	In this work, we set out to identify the genes involved in EPS production in these L. plantarum strains and to demonstrate their role in EPS production by gene deletion analysis.
27107126	10	23	theme	bacteria	2055:2062	arg1	properties					2035:2044	the cell surface properties	2018:2044	the cell surface properties of these bacteria	2018:2062	IMPORTANCE This study evaluates the role of extracellular polysaccharides that are produced by different strains of Lactobacillus plantarum in the determination of the cell surface properties of these bacteria and their capacity to interact with their environment, including their signaling to human host cells.
27107126	9	24	theme	L.	1779:1780	arg1	strains					1792:1798	L. plantarum strains	1779:1798	L. plantarum strains	1779:1798	Taken together, these experiments illustrate the importance of EPS in L. plantarum strains as a strain-specific determinant in host interaction.
27107126	8	25	theme	receptor	1486:1493	arg1	signaling					1504:1512	Toll-like receptor 2 (TLR2) signaling	1476:1512	Toll-like receptor 2 (TLR2) signaling	1476:1512	In addition, the Toll-like receptor 2 (TLR2) signaling and immunomodulatory capacities of the EPS-negative derivatives and their respective wild-type strains were compared, revealing strain-specific impacts of EPS on the immunomodulatory properties.
27107126	6	26	theme	cps2-like	1047:1055	arg1	clusters					1062:1069	cps2-like gene clusters	1047:1069	cps2-like gene clusters in SF2A35B and Lp90 responsible for major EPS production	1047:1126	The constructed EPS-reduced derivatives were analyzed for the abundance and sugar compositions of their EPS, revealing cps2-like gene clusters in SF2A35B and Lp90 responsible for major EPS production.
27107126	11	27	theme	diverse	2292:2298	arg1	roles					2318:2322	the diverse and unpredictable roles	2288:2322	the diverse and unpredictable roles of these polysaccharides in the environmental interactions of these bacterial strains	2288:2408	The results clearly show that the consequences of removal of these polysaccharides are very strain specific, illustrating the diverse and unpredictable roles of these polysaccharides in the environmental interactions of these bacterial strains.
27107126	5	28	theme	model	780:784	arg1	WCFS1					807:811	WCFS1	807:811	WCFS1	807:811	A model L. plantarum strain, WCFS1, and its previously constructed derivative that produced reduced levels of EPS were included as reference strains.
27107126	5	28	theme	model	780:784	arg1	strain					799:804	A model L. plantarum strain	778:804	A model L. plantarum strain	778:804	A model L. plantarum strain, WCFS1, and its previously constructed derivative that produced reduced levels of EPS were included as reference strains.
27107126	5	28	theme	model	780:784	arg1	strains					919:925	reference strains	909:925	reference strains	909:925	A model L. plantarum strain, WCFS1, and its previously constructed derivative that produced reduced levels of EPS were included as reference strains.
27107126	5	28	theme	model	780:784	arg1	derivative					845:854	its previously constructed derivative	818:854	its previously constructed derivative that produced reduced levels of EPS	818:890	A model L. plantarum strain, WCFS1, and its previously constructed derivative that produced reduced levels of EPS were included as reference strains.
27107126	0	29	theme	plantarum-Host	92:105	arg1	Interactions					107:118	Lactobacillus plantarum-Host Interactions	78:118	Lactobacillus plantarum-Host Interactions	78:118	Strain-Specific Features of Extracellular Polysaccharides and Their Impact on Lactobacillus plantarum-Host Interactions.
27107126	9	30	theme	host	1836:1839	arg1	interaction					1841:1851	host interaction	1836:1851	host interaction	1836:1851	Taken together, these experiments illustrate the importance of EPS in L. plantarum strains as a strain-specific determinant in host interaction.
27107126	11	31	theme	polysaccharides	2233:2247	arg1	removal					2216:2222	removal	2216:2222	removal of these polysaccharides	2216:2247	The results clearly show that the consequences of removal of these polysaccharides are very strain specific, illustrating the diverse and unpredictable roles of these polysaccharides in the environmental interactions of these bacterial strains.
27107126	4	32	theme	deletion	759:766	arg1	analysis					768:775	gene deletion analysis	754:775	gene deletion analysis	754:775	In this work, we set out to identify the genes involved in EPS production in these L. plantarum strains and to demonstrate their role in EPS production by gene deletion analysis.
27107126	0	33	theme	Strain-Specific	0:14	arg1	Features					16:23	Strain-Specific Features	0:23	Strain-Specific Features of Extracellular Polysaccharides	0:56	Strain-Specific Features of Extracellular Polysaccharides and Their Impact on Lactobacillus plantarum-Host Interactions.
27107126	1	34	theme	UNLABELLED	121:130	arg1	supplements					238:248	probiotic, health-promoting food supplements	205:248	probiotic, health-promoting food supplements	205:248	UNLABELLED Lactobacilli are found in diverse environments and are widely applied as probiotic, health-promoting food supplements.
27107126	1	34	theme	UNLABELLED	121:130	arg1	Lactobacilli					132:143	UNLABELLED Lactobacilli	121:143	UNLABELLED Lactobacilli	121:143	UNLABELLED Lactobacilli are found in diverse environments and are widely applied as probiotic, health-promoting food supplements.
27107126	8	35	theme	derivatives	1566:1576	arg1	signaling					1504:1512	Toll-like receptor 2 (TLR2) signaling	1476:1512	Toll-like receptor 2 (TLR2) signaling	1476:1512	In addition, the Toll-like receptor 2 (TLR2) signaling and immunomodulatory capacities of the EPS-negative derivatives and their respective wild-type strains were compared, revealing strain-specific impacts of EPS on the immunomodulatory properties.
27107126	8	35	theme	derivatives	1566:1576	arg1	capacities					1535:1544	immunomodulatory capacities	1518:1544	immunomodulatory capacities	1518:1544	In addition, the Toll-like receptor 2 (TLR2) signaling and immunomodulatory capacities of the EPS-negative derivatives and their respective wild-type strains were compared, revealing strain-specific impacts of EPS on the immunomodulatory properties.
27107126	3	36	theme	ropy	515:518	arg1	phenotype					520:528	an obvious ropy phenotype	504:528	an obvious ropy phenotype	504:528	Two Lactobacillus plantarum strains, SF2A35B and Lp90, have an obvious ropy phenotype, implying high extracellular polysaccharide (EPS) production levels.
27107126	5	37	theme	constructed	833:843	arg1	WCFS1					807:811	WCFS1	807:811	WCFS1	807:811	A model L. plantarum strain, WCFS1, and its previously constructed derivative that produced reduced levels of EPS were included as reference strains.
27107126	5	37	theme	constructed	833:843	arg1	derivative					845:854	its previously constructed derivative	818:854	its previously constructed derivative that produced reduced levels of EPS	818:890	A model L. plantarum strain, WCFS1, and its previously constructed derivative that produced reduced levels of EPS were included as reference strains.
27107126	5	37	theme	constructed	833:843	arg1	strains					919:925	reference strains	909:925	reference strains	909:925	A model L. plantarum strain, WCFS1, and its previously constructed derivative that produced reduced levels of EPS were included as reference strains.
27107126	5	37	theme	constructed	833:843	arg1	strain					799:804	A model L. plantarum strain	778:804	A model L. plantarum strain	778:804	A model L. plantarum strain, WCFS1, and its previously constructed derivative that produced reduced levels of EPS were included as reference strains.
27107126	10	38	from	capacity	2074:2081	arg1	signaling					2135:2143	their signaling	2129:2143	their signaling to human host cells	2129:2163	IMPORTANCE This study evaluates the role of extracellular polysaccharides that are produced by different strains of Lactobacillus plantarum in the determination of the cell surface properties of these bacteria and their capacity to interact with their environment, including their signaling to human host cells.
27107126	10	38	from	capacity	2074:2081	arg1	strains					1959:1965	different strains	1949:1965	different strains	1949:1965	IMPORTANCE This study evaluates the role of extracellular polysaccharides that are produced by different strains of Lactobacillus plantarum in the determination of the cell surface properties of these bacteria and their capacity to interact with their environment, including their signaling to human host cells.
27107126	9	39	from	importance	1758:1767	arg1	strains					1792:1798	L. plantarum strains	1779:1798	L. plantarum strains	1779:1798	Taken together, these experiments illustrate the importance of EPS in L. plantarum strains as a strain-specific determinant in host interaction.
27107126	12	40	theme	specificity	2542:2552	arg1	importance					2521:2530	the importance	2517:2530	the importance of strain specificity	2517:2552	In the context of the use of lactobacilli as health-promoting probiotic organisms, this study exemplifies the importance of strain specificity.
27107126	11	41	from	roles	2318:2322	arg1	interactions					2370:2381	the environmental interactions	2352:2381	the environmental interactions of these bacterial strains	2352:2408	The results clearly show that the consequences of removal of these polysaccharides are very strain specific, illustrating the diverse and unpredictable roles of these polysaccharides in the environmental interactions of these bacterial strains.
27107126	0	42	theme	Polysaccharides	42:56	arg1	Features					16:23	Strain-Specific Features	0:23	Strain-Specific Features of Extracellular Polysaccharides	0:56	Strain-Specific Features of Extracellular Polysaccharides and Their Impact on Lactobacillus plantarum-Host Interactions.
27107126	0	42	theme	Polysaccharides	42:56	arg1	Impact					68:73	Their Impact	62:73	Their Impact on Lactobacillus plantarum-Host Interactions	62:118	Strain-Specific Features of Extracellular Polysaccharides and Their Impact on Lactobacillus plantarum-Host Interactions.
27107126	7	43	theme	phenotypic	1176:1185	arg1	characteristics					1187:1201	phenotypic characteristics	1176:1201	phenotypic characteristics that are of relevance for their capacity to interact with the host epithelium in the intestinal tract, including bacterial surface properties as well as survival under the stress conditions encountered in the gastrointestinal tract (acid and bile stress)	1176:1456	Moreover, these mutant strains were tested for phenotypic characteristics that are of relevance for their capacity to interact with the host epithelium in the intestinal tract, including bacterial surface properties as well as survival under the stress conditions encountered in the gastrointestinal tract (acid and bile stress).
27107126	9	44	from	determinant	1821:1831	arg1	interaction					1841:1851	host interaction	1836:1851	host interaction	1836:1851	Taken together, these experiments illustrate the importance of EPS in L. plantarum strains as a strain-specific determinant in host interaction.
27107126	7	45	theme	surface	1326:1332	arg1	properties					1334:1343	bacterial surface properties	1316:1343	bacterial surface properties as well as survival	1316:1363	Moreover, these mutant strains were tested for phenotypic characteristics that are of relevance for their capacity to interact with the host epithelium in the intestinal tract, including bacterial surface properties as well as survival under the stress conditions encountered in the gastrointestinal tract (acid and bile stress).
27107126	3	46	theme	plantarum	462:470	arg1	strains					472:478	Two Lactobacillus plantarum strains	444:478	Two Lactobacillus plantarum strains	444:478	Two Lactobacillus plantarum strains, SF2A35B and Lp90, have an obvious ropy phenotype, implying high extracellular polysaccharide (EPS) production levels.
27107126	3	46	theme	plantarum	462:470	arg1	Lp90					493:496	Lp90	493:496	Lp90	493:496	Two Lactobacillus plantarum strains, SF2A35B and Lp90, have an obvious ropy phenotype, implying high extracellular polysaccharide (EPS) production levels.
27107126	3	46	theme	plantarum	462:470	arg1	SF2A35B					481:487	SF2A35B	481:487	SF2A35B	481:487	Two Lactobacillus plantarum strains, SF2A35B and Lp90, have an obvious ropy phenotype, implying high extracellular polysaccharide (EPS) production levels.
27107126	8	47	theme	wild-type	1599:1607	arg1	strains					1609:1615	their respective wild-type strains	1582:1615	their respective wild-type strains	1582:1615	In addition, the Toll-like receptor 2 (TLR2) signaling and immunomodulatory capacities of the EPS-negative derivatives and their respective wild-type strains were compared, revealing strain-specific impacts of EPS on the immunomodulatory properties.
27107126	10	48	theme	extracellular	1898:1910	arg1	polysaccharides					1912:1926	extracellular polysaccharides	1898:1926	extracellular polysaccharides that are produced by different strains of Lactobacillus plantarum in the determination of the cell surface properties of these bacteria and their capacity to interact with their environment, including their signaling to human host cells	1898:2163	IMPORTANCE This study evaluates the role of extracellular polysaccharides that are produced by different strains of Lactobacillus plantarum in the determination of the cell surface properties of these bacteria and their capacity to interact with their environment, including their signaling to human host cells.
27107126	3	49	theme	high	540:543	arg1	levels					591:596	high extracellular polysaccharide (EPS) production levels	540:596	high extracellular polysaccharide (EPS) production levels	540:596	Two Lactobacillus plantarum strains, SF2A35B and Lp90, have an obvious ropy phenotype, implying high extracellular polysaccharide (EPS) production levels.
27107126	11	50	theme	removal	2216:2222	arg1	consequences					2200:2211	the consequences	2196:2211	the consequences of removal of these polysaccharides	2196:2247	The results clearly show that the consequences of removal of these polysaccharides are very strain specific, illustrating the diverse and unpredictable roles of these polysaccharides in the environmental interactions of these bacterial strains.
27107126	11	50	theme	removal	2216:2222	arg1	specific					2265:2272	specific	2265:2272	specific	2265:2272	The results clearly show that the consequences of removal of these polysaccharides are very strain specific, illustrating the diverse and unpredictable roles of these polysaccharides in the environmental interactions of these bacterial strains.
27107126	10	51	theme	human	2148:2152	arg1	cells					2159:2163	human host cells	2148:2163	human host cells	2148:2163	IMPORTANCE This study evaluates the role of extracellular polysaccharides that are produced by different strains of Lactobacillus plantarum in the determination of the cell surface properties of these bacteria and their capacity to interact with their environment, including their signaling to human host cells.
27107126	10	52	theme	different	1949:1957	arg1	signaling					2135:2143	their signaling	2129:2143	their signaling to human host cells	2129:2163	IMPORTANCE This study evaluates the role of extracellular polysaccharides that are produced by different strains of Lactobacillus plantarum in the determination of the cell surface properties of these bacteria and their capacity to interact with their environment, including their signaling to human host cells.
27107126	10	52	theme	different	1949:1957	arg1	strains					1959:1965	different strains	1949:1965	different strains	1949:1965	IMPORTANCE This study evaluates the role of extracellular polysaccharides that are produced by different strains of Lactobacillus plantarum in the determination of the cell surface properties of these bacteria and their capacity to interact with their environment, including their signaling to human host cells.
27107126	8	53	theme	immunomodulatory	1680:1695	arg1	properties					1697:1706	the immunomodulatory properties	1676:1706	the immunomodulatory properties	1676:1706	In addition, the Toll-like receptor 2 (TLR2) signaling and immunomodulatory capacities of the EPS-negative derivatives and their respective wild-type strains were compared, revealing strain-specific impacts of EPS on the immunomodulatory properties.
27107126	11	54	theme	strains	2402:2408	arg1	interactions					2370:2381	the environmental interactions	2352:2381	the environmental interactions of these bacterial strains	2352:2408	The results clearly show that the consequences of removal of these polysaccharides are very strain specific, illustrating the diverse and unpredictable roles of these polysaccharides in the environmental interactions of these bacterial strains.
27107126	12	55	theme	probiotic	2473:2481	arg1	organisms					2483:2491	health-promoting probiotic organisms	2456:2491	health-promoting probiotic organisms	2456:2491	In the context of the use of lactobacilli as health-promoting probiotic organisms, this study exemplifies the importance of strain specificity.
27107126	6	56	theme	EPS-reduced	944:954	arg1	derivatives					956:966	The constructed EPS-reduced derivatives	928:966	The constructed EPS-reduced derivatives	928:966	The constructed EPS-reduced derivatives were analyzed for the abundance and sugar compositions of their EPS, revealing cps2-like gene clusters in SF2A35B and Lp90 responsible for major EPS production.
27107126	8	57	theme	strain-specific	1642:1656	arg1	impacts					1658:1664	strain-specific impacts	1642:1664	strain-specific impacts of EPS on the immunomodulatory properties	1642:1706	In addition, the Toll-like receptor 2 (TLR2) signaling and immunomodulatory capacities of the EPS-negative derivatives and their respective wild-type strains were compared, revealing strain-specific impacts of EPS on the immunomodulatory properties.
27107126	10	58	theme	plantarum	1984:1992	arg1	signaling					2135:2143	their signaling	2129:2143	their signaling to human host cells	2129:2163	IMPORTANCE This study evaluates the role of extracellular polysaccharides that are produced by different strains of Lactobacillus plantarum in the determination of the cell surface properties of these bacteria and their capacity to interact with their environment, including their signaling to human host cells.
27107126	10	58	theme	plantarum	1984:1992	arg1	strains					1959:1965	different strains	1949:1965	different strains	1949:1965	IMPORTANCE This study evaluates the role of extracellular polysaccharides that are produced by different strains of Lactobacillus plantarum in the determination of the cell surface properties of these bacteria and their capacity to interact with their environment, including their signaling to human host cells.
27107126	7	59	theme	mutant	1145:1150	arg1	strains					1152:1158	these mutant strains	1139:1158	these mutant strains	1139:1158	Moreover, these mutant strains were tested for phenotypic characteristics that are of relevance for their capacity to interact with the host epithelium in the intestinal tract, including bacterial surface properties as well as survival under the stress conditions encountered in the gastrointestinal tract (acid and bile stress).
27107126	3	60	theme	polysaccharide	559:572	arg1	levels					591:596	high extracellular polysaccharide (EPS) production levels	540:596	high extracellular polysaccharide (EPS) production levels	540:596	Two Lactobacillus plantarum strains, SF2A35B and Lp90, have an obvious ropy phenotype, implying high extracellular polysaccharide (EPS) production levels.
27107126	6	61	theme	EPS	1113:1115	arg1	production					1117:1126	major EPS production	1107:1126	major EPS production	1107:1126	The constructed EPS-reduced derivatives were analyzed for the abundance and sugar compositions of their EPS, revealing cps2-like gene clusters in SF2A35B and Lp90 responsible for major EPS production.
27107126	8	62	dep	signaling	1504:1512	arg1	the					1472:1474	the	1472:1474	the	1472:1474	In addition, the Toll-like receptor 2 (TLR2) signaling and immunomodulatory capacities of the EPS-negative derivatives and their respective wild-type strains were compared, revealing strain-specific impacts of EPS on the immunomodulatory properties.
27107126	2	63	theme	probiotic	397:405	arg1	effects					407:413	probiotic effects	397:413	probiotic effects	397:413	Polysaccharides are ubiquitously present on the cell surface of lactobacilli and are considered to contribute to the species- and strain-specific probiotic effects that are typically observed.
27107126	3	64	theme	production	580:589	arg1	levels					591:596	high extracellular polysaccharide (EPS) production levels	540:596	high extracellular polysaccharide (EPS) production levels	540:596	Two Lactobacillus plantarum strains, SF2A35B and Lp90, have an obvious ropy phenotype, implying high extracellular polysaccharide (EPS) production levels.
27107126	6	65	theme	EPS	1032:1034	arg1	compositions					1010:1021	the abundance and sugar compositions	986:1021	compositions	1010:1021	The constructed EPS-reduced derivatives were analyzed for the abundance and sugar compositions of their EPS, revealing cps2-like gene clusters in SF2A35B and Lp90 responsible for major EPS production.
27107126	11	66	theme	polysaccharides	2333:2347	arg1	roles					2318:2322	the diverse and unpredictable roles	2288:2322	the diverse and unpredictable roles of these polysaccharides in the environmental interactions of these bacterial strains	2288:2408	The results clearly show that the consequences of removal of these polysaccharides are very strain specific, illustrating the diverse and unpredictable roles of these polysaccharides in the environmental interactions of these bacterial strains.
27107126	4	67	theme	L.	682:683	arg1	strains					695:701	these L. plantarum strains	676:701	these L. plantarum strains	676:701	In this work, we set out to identify the genes involved in EPS production in these L. plantarum strains and to demonstrate their role in EPS production by gene deletion analysis.
27107126	5	68	theme	plantarum	789:797	arg1	WCFS1					807:811	WCFS1	807:811	WCFS1	807:811	A model L. plantarum strain, WCFS1, and its previously constructed derivative that produced reduced levels of EPS were included as reference strains.
27107126	5	68	theme	plantarum	789:797	arg1	strain					799:804	A model L. plantarum strain	778:804	A model L. plantarum strain	778:804	A model L. plantarum strain, WCFS1, and its previously constructed derivative that produced reduced levels of EPS were included as reference strains.
27107126	5	68	theme	plantarum	789:797	arg1	strains					919:925	reference strains	909:925	reference strains	909:925	A model L. plantarum strain, WCFS1, and its previously constructed derivative that produced reduced levels of EPS were included as reference strains.
27107126	5	68	theme	plantarum	789:797	arg1	derivative					845:854	its previously constructed derivative	818:854	its previously constructed derivative that produced reduced levels of EPS	818:890	A model L. plantarum strain, WCFS1, and its previously constructed derivative that produced reduced levels of EPS were included as reference strains.
27107126	6	69	from	clusters	1062:1069	arg1	SF2A35B					1074:1080	SF2A35B	1074:1080	SF2A35B	1074:1080	The constructed EPS-reduced derivatives were analyzed for the abundance and sugar compositions of their EPS, revealing cps2-like gene clusters in SF2A35B and Lp90 responsible for major EPS production.
27107126	6	69	from	clusters	1062:1069	arg1	Lp90					1086:1089	Lp90	1086:1089	Lp90	1086:1089	The constructed EPS-reduced derivatives were analyzed for the abundance and sugar compositions of their EPS, revealing cps2-like gene clusters in SF2A35B and Lp90 responsible for major EPS production.
27107126	5	70	theme	EPS	888:890	arg1	levels					878:883	reduced levels	870:883	reduced levels of EPS	870:890	A model L. plantarum strain, WCFS1, and its previously constructed derivative that produced reduced levels of EPS were included as reference strains.
27107126	8	71	theme	immunomodulatory	1518:1533	arg1	capacities					1535:1544	immunomodulatory capacities	1518:1544	immunomodulatory capacities	1518:1544	In addition, the Toll-like receptor 2 (TLR2) signaling and immunomodulatory capacities of the EPS-negative derivatives and their respective wild-type strains were compared, revealing strain-specific impacts of EPS on the immunomodulatory properties.
27107126	6	72	theme	sugar	1004:1008	arg1	compositions					1010:1021	the abundance and sugar compositions	986:1021	compositions	1010:1021	The constructed EPS-reduced derivatives were analyzed for the abundance and sugar compositions of their EPS, revealing cps2-like gene clusters in SF2A35B and Lp90 responsible for major EPS production.
27107126	7	73	theme	bile	1445:1448	arg1	stress					1450:1455	bile stress	1445:1455	bile stress	1445:1455	Moreover, these mutant strains were tested for phenotypic characteristics that are of relevance for their capacity to interact with the host epithelium in the intestinal tract, including bacterial surface properties as well as survival under the stress conditions encountered in the gastrointestinal tract (acid and bile stress).
27107126	10	74	theme	properties	2035:2044	arg1	determination					2001:2013	the determination	1997:2013	the determination of the cell surface properties of these bacteria	1997:2062	IMPORTANCE This study evaluates the role of extracellular polysaccharides that are produced by different strains of Lactobacillus plantarum in the determination of the cell surface properties of these bacteria and their capacity to interact with their environment, including their signaling to human host cells.
27107126	10	74	theme	properties	2035:2044	arg1	capacity					2074:2081	their capacity to interact with their environment	2068:2116	their capacity to interact with their environment	2068:2116	IMPORTANCE This study evaluates the role of extracellular polysaccharides that are produced by different strains of Lactobacillus plantarum in the determination of the cell surface properties of these bacteria and their capacity to interact with their environment, including their signaling to human host cells.
27107126	8	75	from	impacts	1658:1664	arg1	properties					1697:1706	the immunomodulatory properties	1676:1706	the immunomodulatory properties	1676:1706	In addition, the Toll-like receptor 2 (TLR2) signaling and immunomodulatory capacities of the EPS-negative derivatives and their respective wild-type strains were compared, revealing strain-specific impacts of EPS on the immunomodulatory properties.
27107126	1	76	theme	food	233:236	arg1	supplements					238:248	probiotic, health-promoting food supplements	205:248	probiotic, health-promoting food supplements	205:248	UNLABELLED Lactobacilli are found in diverse environments and are widely applied as probiotic, health-promoting food supplements.
27107126	1	76	theme	food	233:236	arg1	Lactobacilli					132:143	UNLABELLED Lactobacilli	121:143	UNLABELLED Lactobacilli	121:143	UNLABELLED Lactobacilli are found in diverse environments and are widely applied as probiotic, health-promoting food supplements.
27107126	6	77	theme	abundance	990:998	arg1	compositions					1010:1021	the abundance and sugar compositions	986:1021	compositions	1010:1021	The constructed EPS-reduced derivatives were analyzed for the abundance and sugar compositions of their EPS, revealing cps2-like gene clusters in SF2A35B and Lp90 responsible for major EPS production.
27107126	4	78	theme	EPS	658:660	arg1	production					662:671	EPS production	658:671	EPS production in these L. plantarum strains	658:701	In this work, we set out to identify the genes involved in EPS production in these L. plantarum strains and to demonstrate their role in EPS production by gene deletion analysis.
27107126	10	79	theme	cell	2022:2025	arg1	properties					2035:2044	the cell surface properties	2018:2044	the cell surface properties of these bacteria	2018:2062	IMPORTANCE This study evaluates the role of extracellular polysaccharides that are produced by different strains of Lactobacillus plantarum in the determination of the cell surface properties of these bacteria and their capacity to interact with their environment, including their signaling to human host cells.
27107126	8	80	theme	Toll-like	1476:1484	arg1	receptor					1486:1493	Toll-like receptor 2	1476:1495	Toll-like receptor 2 (TLR2) signaling	1476:1512	In addition, the Toll-like receptor 2 (TLR2) signaling and immunomodulatory capacities of the EPS-negative derivatives and their respective wild-type strains were compared, revealing strain-specific impacts of EPS on the immunomodulatory properties.
27107126	8	80	theme	Toll-like	1476:1484	arg1	TLR2					1498:1501	TLR2	1498:1501	TLR2	1498:1501	In addition, the Toll-like receptor 2 (TLR2) signaling and immunomodulatory capacities of the EPS-negative derivatives and their respective wild-type strains were compared, revealing strain-specific impacts of EPS on the immunomodulatory properties.
27107126	1	81	theme	diverse	158:164	arg1	environments					166:177	diverse environments	158:177	diverse environments	158:177	UNLABELLED Lactobacilli are found in diverse environments and are widely applied as probiotic, health-promoting food supplements.
27107126	6	82	theme	gene	1057:1060	arg1	clusters					1062:1069	cps2-like gene clusters	1047:1069	cps2-like gene clusters in SF2A35B and Lp90 responsible for major EPS production	1047:1126	The constructed EPS-reduced derivatives were analyzed for the abundance and sugar compositions of their EPS, revealing cps2-like gene clusters in SF2A35B and Lp90 responsible for major EPS production.
27107126	9	83	theme	plantarum	1782:1790	arg1	strains					1792:1798	L. plantarum strains	1779:1798	L. plantarum strains	1779:1798	Taken together, these experiments illustrate the importance of EPS in L. plantarum strains as a strain-specific determinant in host interaction.
27107126	3	84	contain	have	499:502	arg2	phenotype					520:528	an obvious ropy phenotype	504:528	an obvious ropy phenotype	504:528	Two Lactobacillus plantarum strains, SF2A35B and Lp90, have an obvious ropy phenotype, implying high extracellular polysaccharide (EPS) production levels.
27107126	3	84	contain	have	499:502	arg1	strains					472:478	Two Lactobacillus plantarum strains	444:478	Two Lactobacillus plantarum strains	444:478	Two Lactobacillus plantarum strains, SF2A35B and Lp90, have an obvious ropy phenotype, implying high extracellular polysaccharide (EPS) production levels.
27107126	3	84	contain	have	499:502	arg1	Lp90					493:496	Lp90	493:496	Lp90	493:496	Two Lactobacillus plantarum strains, SF2A35B and Lp90, have an obvious ropy phenotype, implying high extracellular polysaccharide (EPS) production levels.
27107126	3	84	contain	have	499:502	arg1	SF2A35B					481:487	SF2A35B	481:487	SF2A35B	481:487	Two Lactobacillus plantarum strains, SF2A35B and Lp90, have an obvious ropy phenotype, implying high extracellular polysaccharide (EPS) production levels.
27107126	0	85	theme	Lactobacillus	78:90	arg1	Interactions					107:118	Lactobacillus plantarum-Host Interactions	78:118	Lactobacillus plantarum-Host Interactions	78:118	Strain-Specific Features of Extracellular Polysaccharides and Their Impact on Lactobacillus plantarum-Host Interactions.
27107126	2	86	theme	cell	299:302	arg1	surface					304:310	the cell surface	295:310	the cell surface of lactobacilli	295:326	Polysaccharides are ubiquitously present on the cell surface of lactobacilli and are considered to contribute to the species- and strain-specific probiotic effects that are typically observed.
27107126	7	87	theme	stress	1375:1380	arg1	conditions					1382:1391	the stress conditions	1371:1391	the stress conditions encountered in the gastrointestinal tract (acid and bile stress)	1371:1456	Moreover, these mutant strains were tested for phenotypic characteristics that are of relevance for their capacity to interact with the host epithelium in the intestinal tract, including bacterial surface properties as well as survival under the stress conditions encountered in the gastrointestinal tract (acid and bile stress).
27107126	5	88	theme	L.	786:787	arg1	WCFS1					807:811	WCFS1	807:811	WCFS1	807:811	A model L. plantarum strain, WCFS1, and its previously constructed derivative that produced reduced levels of EPS were included as reference strains.
27107126	5	88	theme	L.	786:787	arg1	strain					799:804	A model L. plantarum strain	778:804	A model L. plantarum strain	778:804	A model L. plantarum strain, WCFS1, and its previously constructed derivative that produced reduced levels of EPS were included as reference strains.
27107126	5	88	theme	L.	786:787	arg1	strains					919:925	reference strains	909:925	reference strains	909:925	A model L. plantarum strain, WCFS1, and its previously constructed derivative that produced reduced levels of EPS were included as reference strains.
27107126	5	88	theme	L.	786:787	arg1	derivative					845:854	its previously constructed derivative	818:854	its previously constructed derivative that produced reduced levels of EPS	818:890	A model L. plantarum strain, WCFS1, and its previously constructed derivative that produced reduced levels of EPS were included as reference strains.
27107126	1	89	located	found	149:153	arg2	supplements					238:248	probiotic, health-promoting food supplements	205:248	probiotic, health-promoting food supplements	205:248	UNLABELLED Lactobacilli are found in diverse environments and are widely applied as probiotic, health-promoting food supplements.
27107126	1	89	located	found	149:153	arg1	environments					166:177	diverse environments	158:177	diverse environments	158:177	UNLABELLED Lactobacilli are found in diverse environments and are widely applied as probiotic, health-promoting food supplements.
27107126	1	89	located	found	149:153	arg2	Lactobacilli					132:143	UNLABELLED Lactobacilli	121:143	UNLABELLED Lactobacilli	121:143	UNLABELLED Lactobacilli are found in diverse environments and are widely applied as probiotic, health-promoting food supplements.
27107126	5	90	theme	reference	909:917	arg1	WCFS1					807:811	WCFS1	807:811	WCFS1	807:811	A model L. plantarum strain, WCFS1, and its previously constructed derivative that produced reduced levels of EPS were included as reference strains.
27107126	5	90	theme	reference	909:917	arg1	strain					799:804	A model L. plantarum strain	778:804	A model L. plantarum strain	778:804	A model L. plantarum strain, WCFS1, and its previously constructed derivative that produced reduced levels of EPS were included as reference strains.
27107126	5	90	theme	reference	909:917	arg1	strains					919:925	reference strains	909:925	reference strains	909:925	A model L. plantarum strain, WCFS1, and its previously constructed derivative that produced reduced levels of EPS were included as reference strains.
27107126	5	90	theme	reference	909:917	arg1	derivative					845:854	its previously constructed derivative	818:854	its previously constructed derivative that produced reduced levels of EPS	818:890	A model L. plantarum strain, WCFS1, and its previously constructed derivative that produced reduced levels of EPS were included as reference strains.
27107126	2	91	attach	present	284:290	arg2	Polysaccharides					251:265	Polysaccharides	251:265	Polysaccharides	251:265	Polysaccharides are ubiquitously present on the cell surface of lactobacilli and are considered to contribute to the species- and strain-specific probiotic effects that are typically observed.
27107126	2	91	attach	present	284:290	arg1	surface					304:310	the cell surface	295:310	the cell surface of lactobacilli	295:326	Polysaccharides are ubiquitously present on the cell surface of lactobacilli and are considered to contribute to the species- and strain-specific probiotic effects that are typically observed.
27107126	9	92	theme	strain-specific	1805:1819	arg1	importance					1758:1767	the importance	1754:1767	the importance of EPS in L. plantarum strains	1754:1798	Taken together, these experiments illustrate the importance of EPS in L. plantarum strains as a strain-specific determinant in host interaction.
27107126	9	92	theme	strain-specific	1805:1819	arg1	determinant					1821:1831	a strain-specific determinant	1803:1831	a strain-specific determinant in host interaction	1803:1851	Taken together, these experiments illustrate the importance of EPS in L. plantarum strains as a strain-specific determinant in host interaction.
27107126	0	93	from	Features	16:23	arg1	Interactions					107:118	Lactobacillus plantarum-Host Interactions	78:118	Lactobacillus plantarum-Host Interactions	78:118	Strain-Specific Features of Extracellular Polysaccharides and Their Impact on Lactobacillus plantarum-Host Interactions.
27107126	12	94	theme	lactobacilli	2440:2451	arg1	use					2433:2435	the use	2429:2435	the use of lactobacilli as health-promoting probiotic organisms	2429:2491	In the context of the use of lactobacilli as health-promoting probiotic organisms, this study exemplifies the importance of strain specificity.
27107126	0	95	from	Impact	68:73	arg1	Interactions					107:118	Lactobacillus plantarum-Host Interactions	78:118	Lactobacillus plantarum-Host Interactions	78:118	Strain-Specific Features of Extracellular Polysaccharides and Their Impact on Lactobacillus plantarum-Host Interactions.
27107126	6	96	theme	major	1107:1111	arg1	production					1117:1126	major EPS production	1107:1126	major EPS production	1107:1126	The constructed EPS-reduced derivatives were analyzed for the abundance and sugar compositions of their EPS, revealing cps2-like gene clusters in SF2A35B and Lp90 responsible for major EPS production.
27107126	8	97	theme	EPS-negative	1553:1564	arg1	derivatives					1566:1576	the EPS-negative derivatives	1549:1576	the EPS-negative derivatives	1549:1576	In addition, the Toll-like receptor 2 (TLR2) signaling and immunomodulatory capacities of the EPS-negative derivatives and their respective wild-type strains were compared, revealing strain-specific impacts of EPS on the immunomodulatory properties.
27107126	11	98	theme	unpredictable	2304:2316	arg1	roles					2318:2322	the diverse and unpredictable roles	2288:2322	the diverse and unpredictable roles of these polysaccharides in the environmental interactions of these bacterial strains	2288:2408	The results clearly show that the consequences of removal of these polysaccharides are very strain specific, illustrating the diverse and unpredictable roles of these polysaccharides in the environmental interactions of these bacterial strains.
27107126	6	99	theme	responsible	1091:1101	arg1	clusters					1062:1069	cps2-like gene clusters	1047:1069	cps2-like gene clusters in SF2A35B and Lp90 responsible for major EPS production	1047:1126	The constructed EPS-reduced derivatives were analyzed for the abundance and sugar compositions of their EPS, revealing cps2-like gene clusters in SF2A35B and Lp90 responsible for major EPS production.
27107126	3	100	theme	Lactobacillus	448:460	arg1	strains					472:478	Two Lactobacillus plantarum strains	444:478	Two Lactobacillus plantarum strains	444:478	Two Lactobacillus plantarum strains, SF2A35B and Lp90, have an obvious ropy phenotype, implying high extracellular polysaccharide (EPS) production levels.
27107126	3	100	theme	Lactobacillus	448:460	arg1	Lp90					493:496	Lp90	493:496	Lp90	493:496	Two Lactobacillus plantarum strains, SF2A35B and Lp90, have an obvious ropy phenotype, implying high extracellular polysaccharide (EPS) production levels.
27107126	3	100	theme	Lactobacillus	448:460	arg1	SF2A35B					481:487	SF2A35B	481:487	SF2A35B	481:487	Two Lactobacillus plantarum strains, SF2A35B and Lp90, have an obvious ropy phenotype, implying high extracellular polysaccharide (EPS) production levels.
27107126	12	101	theme	strain	2535:2540	arg1	specificity					2542:2552	strain specificity	2535:2552	strain specificity	2535:2552	In the context of the use of lactobacilli as health-promoting probiotic organisms, this study exemplifies the importance of strain specificity.
27107126	4	102	theme	gene	754:757	arg1	analysis					768:775	gene deletion analysis	754:775	gene deletion analysis	754:775	In this work, we set out to identify the genes involved in EPS production in these L. plantarum strains and to demonstrate their role in EPS production by gene deletion analysis.
27107126	0	103	theme	Extracellular	28:40	arg1	Polysaccharides					42:56	Extracellular Polysaccharides	28:56	Extracellular Polysaccharides	28:56	Strain-Specific Features of Extracellular Polysaccharides and Their Impact on Lactobacillus plantarum-Host Interactions.
27107126	8	104	theme	respective	1588:1597	arg1	strains					1609:1615	their respective wild-type strains	1582:1615	their respective wild-type strains	1582:1615	In addition, the Toll-like receptor 2 (TLR2) signaling and immunomodulatory capacities of the EPS-negative derivatives and their respective wild-type strains were compared, revealing strain-specific impacts of EPS on the immunomodulatory properties.
27107126	7	105	theme	bacterial	1316:1324	arg1	properties					1334:1343	bacterial surface properties	1316:1343	bacterial surface properties as well as survival	1316:1363	Moreover, these mutant strains were tested for phenotypic characteristics that are of relevance for their capacity to interact with the host epithelium in the intestinal tract, including bacterial surface properties as well as survival under the stress conditions encountered in the gastrointestinal tract (acid and bile stress).
27107126	8	106	theme	strains	1609:1615	arg1	signaling					1504:1512	Toll-like receptor 2 (TLR2) signaling	1476:1512	Toll-like receptor 2 (TLR2) signaling	1476:1512	In addition, the Toll-like receptor 2 (TLR2) signaling and immunomodulatory capacities of the EPS-negative derivatives and their respective wild-type strains were compared, revealing strain-specific impacts of EPS on the immunomodulatory properties.
27107126	8	106	theme	strains	1609:1615	arg1	capacities					1535:1544	immunomodulatory capacities	1518:1544	immunomodulatory capacities	1518:1544	In addition, the Toll-like receptor 2 (TLR2) signaling and immunomodulatory capacities of the EPS-negative derivatives and their respective wild-type strains were compared, revealing strain-specific impacts of EPS on the immunomodulatory properties.
27107126	10	107	theme	polysaccharides	1912:1926	arg1	role					1890:1893	the role	1886:1893	the role of extracellular polysaccharides that are produced by different strains of Lactobacillus plantarum in the determination of the cell surface properties of these bacteria and their capacity to interact with their environment, including their signaling to human host cells	1886:2163	IMPORTANCE This study evaluates the role of extracellular polysaccharides that are produced by different strains of Lactobacillus plantarum in the determination of the cell surface properties of these bacteria and their capacity to interact with their environment, including their signaling to human host cells.
27107126	3	108	theme	obvious	507:513	arg1	phenotype					520:528	an obvious ropy phenotype	504:528	an obvious ropy phenotype	504:528	Two Lactobacillus plantarum strains, SF2A35B and Lp90, have an obvious ropy phenotype, implying high extracellular polysaccharide (EPS) production levels.
27107126	1	109	dep	probiotic	205:213	arg1	health-promoting					216:231	health-promoting	216:231	health-promoting	216:231	UNLABELLED Lactobacilli are found in diverse environments and are widely applied as probiotic, health-promoting food supplements.
27107126	12	110	theme	use	2433:2435	arg1	context					2418:2424	the context	2414:2424	the context of the use of lactobacilli as health-promoting probiotic organisms	2414:2491	In the context of the use of lactobacilli as health-promoting probiotic organisms, this study exemplifies the importance of strain specificity.
27107126	3	111	theme	extracellular	545:557	arg1	EPS					575:577	EPS	575:577	EPS	575:577	Two Lactobacillus plantarum strains, SF2A35B and Lp90, have an obvious ropy phenotype, implying high extracellular polysaccharide (EPS) production levels.
27107126	3	111	theme	extracellular	545:557	arg1	polysaccharide					559:572	extracellular polysaccharide	545:572	high extracellular polysaccharide (EPS) production levels	540:596	Two Lactobacillus plantarum strains, SF2A35B and Lp90, have an obvious ropy phenotype, implying high extracellular polysaccharide (EPS) production levels.
27107126	7	112	theme	intestinal	1288:1297	arg1	tract					1299:1303	the intestinal tract	1284:1303	the intestinal tract	1284:1303	Moreover, these mutant strains were tested for phenotypic characteristics that are of relevance for their capacity to interact with the host epithelium in the intestinal tract, including bacterial surface properties as well as survival under the stress conditions encountered in the gastrointestinal tract (acid and bile stress).
27107126	4	113	from	role	728:731	arg1	production					740:749	EPS production	736:749	EPS production by gene deletion analysis	736:775	In this work, we set out to identify the genes involved in EPS production in these L. plantarum strains and to demonstrate their role in EPS production by gene deletion analysis.
24569843	5	0	theme	adherent	714:721	arg1	compact					755:761	compact	755:761	compact	755:761	This suggested that the adherent mucilage in the mutant was more compact compared with that of the wild type.
24569843	5	0	theme	adherent	714:721	arg1	mucilage					723:730	the adherent mucilage	710:730	the adherent mucilage in the mutant	710:744	This suggested that the adherent mucilage in the mutant was more compact compared with that of the wild type.
24569843	6	1	theme	glucomannan	840:850	arg1	synthesis					852:860	glucomannan synthesis	840:860	glucomannan synthesis	840:860	In accordance with the role of CSLA2 in glucomannan synthesis, csla2-1 mucilage contained 30% less mannosyl and glucosyl content than did the wild type.
24569843	10	2	from	changes	1507:1513	arg1	cellulose					1530:1538	crystalline cellulose	1518:1538	crystalline cellulose	1518:1538	As with the observed changes in crystalline cellulose, the spatial distribution of pectin was also modified in csla2-1 mucilage.
24569843	3	3	located	observed	440:447	arg2	expression					425:434	CELLULOSE SYNTHASE-LIKE A2 (CSLA2; At5g22740) expression	379:434	CELLULOSE SYNTHASE-LIKE A2 (CSLA2; At5g22740) expression	379:434	In this study, CELLULOSE SYNTHASE-LIKE A2 (CSLA2; At5g22740) expression was observed in several seed tissues, including the epidermal cells of developing seed coats.
24569843	3	3	located	observed	440:447	arg1	tissues					465:471	several seed tissues	452:471	several seed tissues	452:471	In this study, CELLULOSE SYNTHASE-LIKE A2 (CSLA2; At5g22740) expression was observed in several seed tissues, including the epidermal cells of developing seed coats.
24569843	3	3	located	observed	440:447	arg1	study					372:376	this study	367:376	this study	367:376	In this study, CELLULOSE SYNTHASE-LIKE A2 (CSLA2; At5g22740) expression was observed in several seed tissues, including the epidermal cells of developing seed coats.
24569843	3	3	located	observed	440:447	arg1	cells					498:502	the epidermal cells	484:502	the epidermal cells of developing seed coats	484:527	In this study, CELLULOSE SYNTHASE-LIKE A2 (CSLA2; At5g22740) expression was observed in several seed tissues, including the epidermal cells of developing seed coats.
24569843	10	4	theme	observed	1498:1505	arg1	changes					1507:1513	the observed changes	1494:1513	the observed changes in crystalline cellulose	1494:1538	As with the observed changes in crystalline cellulose, the spatial distribution of pectin was also modified in csla2-1 mucilage.
24569843	1	5	theme	cell	250:253	arg1	wall					255:258	the plant cell wall	240:258	the plant cell wall	240:258	Mannans are hemicellulosic polysaccharides that are considered to have both structural and storage functions in the plant cell wall.
24569843	7	6	from	polysaccharides	1064:1078	arg1	mucilage					1090:1097	mutant mucilage	1083:1097	mutant mucilage	1083:1097	No appreciable changes in the composition, structure, or macromolecular properties were observed for nonmannan polysaccharides in mutant mucilage.
24569843	8	7	theme	Biochemical	1100:1110	arg1	analysis					1112:1119	Biochemical analysis	1100:1119	Biochemical analysis	1100:1119	Biochemical analysis revealed that cellulose crystallinity was substantially reduced in csla2-1 mucilage; this was supported by the removal of most mucilage cellulose through treatment of csla2-1 seeds with endo-β-glucanase.
24569843	2	8	theme	Arabidopsis	327:337	arg1	thaliana					339:346	Arabidopsis thaliana	327:346	Arabidopsis thaliana	327:346	However, it is not yet known how mannans function in Arabidopsis (Arabidopsis thaliana) seed mucilage.
24569843	2	8	theme	Arabidopsis	327:337	arg1	mucilage					354:361	Arabidopsis (Arabidopsis thaliana) seed mucilage	314:361	Arabidopsis (Arabidopsis thaliana) seed mucilage	314:361	However, it is not yet known how mannans function in Arabidopsis (Arabidopsis thaliana) seed mucilage.
24569843	9	9	from	Mutation	1325:1332	arg1	CSLA2					1337:1341	CSLA2	1337:1341	CSLA2	1337:1341	Mutation in CSLA2 also resulted in altered spatial distribution of cellulose and an absence of birefringent cellulose microfibrils within the adherent mucilage.
24569843	0	10	theme	mucilage	88:95	arg1	structure					97:105	adherent mucilage structure	79:105	adherent mucilage structure	79:105	CELLULOSE SYNTHASE-LIKE A2, a glucomannan synthase, is involved in maintaining adherent mucilage structure in Arabidopsis seed.
24569843	6	11	theme	CSLA2	831:835	arg1	role					823:826	the role	819:826	the role of CSLA2 in glucomannan synthesis	819:860	In accordance with the role of CSLA2 in glucomannan synthesis, csla2-1 mucilage contained 30% less mannosyl and glucosyl content than did the wild type.
24569843	8	12	theme	seeds	1296:1300	arg1	treatment					1275:1283	treatment	1275:1283	treatment of csla2-1 seeds with endo-β-glucanase	1275:1322	Biochemical analysis revealed that cellulose crystallinity was substantially reduced in csla2-1 mucilage; this was supported by the removal of most mucilage cellulose through treatment of csla2-1 seeds with endo-β-glucanase.
24569843	6	13	theme	glucosyl	912:919	arg1	content					921:927	glucosyl content	912:927	glucosyl content	912:927	In accordance with the role of CSLA2 in glucomannan synthesis, csla2-1 mucilage contained 30% less mannosyl and glucosyl content than did the wild type.
24569843	0	14	theme	adherent	79:86	arg1	structure					97:105	adherent mucilage structure	79:105	adherent mucilage structure	79:105	CELLULOSE SYNTHASE-LIKE A2, a glucomannan synthase, is involved in maintaining adherent mucilage structure in Arabidopsis seed.
24569843	8	15	theme	csla2-1	1288:1294	arg1	seeds					1296:1300	csla2-1 seeds	1288:1300	csla2-1 seeds	1288:1300	Biochemical analysis revealed that cellulose crystallinity was substantially reduced in csla2-1 mucilage; this was supported by the removal of most mucilage cellulose through treatment of csla2-1 seeds with endo-β-glucanase.
24569843	3	16	theme	developing	507:516	arg1	coats					523:527	developing seed coats	507:527	developing seed coats	507:527	In this study, CELLULOSE SYNTHASE-LIKE A2 (CSLA2; At5g22740) expression was observed in several seed tissues, including the epidermal cells of developing seed coats.
24569843	8	17	theme	most	1243:1246	arg1	cellulose					1257:1265	most mucilage cellulose	1243:1265	most mucilage cellulose	1243:1265	Biochemical analysis revealed that cellulose crystallinity was substantially reduced in csla2-1 mucilage; this was supported by the removal of most mucilage cellulose through treatment of csla2-1 seeds with endo-β-glucanase.
24569843	6	18	dep	did	934:936	arg1	than					929:932	than	929:932	than	929:932	In accordance with the role of CSLA2 in glucomannan synthesis, csla2-1 mucilage contained 30% less mannosyl and glucosyl content than did the wild type.
24569843	9	19	theme	cellulose	1433:1441	arg1	microfibrils					1443:1454	birefringent cellulose microfibrils	1420:1454	birefringent cellulose microfibrils	1420:1454	Mutation in CSLA2 also resulted in altered spatial distribution of cellulose and an absence of birefringent cellulose microfibrils within the adherent mucilage.
24569843	3	20	theme	epidermal	488:496	arg1	cells					498:502	the epidermal cells	484:502	the epidermal cells of developing seed coats	484:527	In this study, CELLULOSE SYNTHASE-LIKE A2 (CSLA2; At5g22740) expression was observed in several seed tissues, including the epidermal cells of developing seed coats.
24569843	4	21	theme	adherent	628:635	arg1	mucilage					637:644	the adherent mucilage	624:644	the adherent mucilage	624:644	Disruption of CSLA2 resulted in thinner adherent mucilage halos, although the total amount of the adherent mucilage did not change compared with the wild type.
24569843	6	22	from	role	823:826	arg1	synthesis					852:860	glucomannan synthesis	840:860	glucomannan synthesis	840:860	In accordance with the role of CSLA2 in glucomannan synthesis, csla2-1 mucilage contained 30% less mannosyl and glucosyl content than did the wild type.
24569843	0	23	theme	glucomannan	30:40	arg1	A2					24:25	CELLULOSE SYNTHASE-LIKE A2	0:25	CELLULOSE SYNTHASE-LIKE A2	0:25	CELLULOSE SYNTHASE-LIKE A2, a glucomannan synthase, is involved in maintaining adherent mucilage structure in Arabidopsis seed.
24569843	0	23	theme	glucomannan	30:40	arg1	synthase					42:49	a glucomannan synthase	28:49	a glucomannan synthase	28:49	CELLULOSE SYNTHASE-LIKE A2, a glucomannan synthase, is involved in maintaining adherent mucilage structure in Arabidopsis seed.
24569843	6	24	theme	wild	942:945	arg1	type					947:950	the wild type	938:950	the wild type	938:950	In accordance with the role of CSLA2 in glucomannan synthesis, csla2-1 mucilage contained 30% less mannosyl and glucosyl content than did the wild type.
24569843	8	25	theme	csla2-1	1188:1194	arg1	mucilage					1196:1203	csla2-1 mucilage	1188:1203	csla2-1 mucilage	1188:1203	Biochemical analysis revealed that cellulose crystallinity was substantially reduced in csla2-1 mucilage; this was supported by the removal of most mucilage cellulose through treatment of csla2-1 seeds with endo-β-glucanase.
24569843	0	26	theme	SYNTHASE-LIKE	10:22	arg1	A2					24:25	CELLULOSE SYNTHASE-LIKE A2	0:25	CELLULOSE SYNTHASE-LIKE A2	0:25	CELLULOSE SYNTHASE-LIKE A2, a glucomannan synthase, is involved in maintaining adherent mucilage structure in Arabidopsis seed.
24569843	0	26	theme	SYNTHASE-LIKE	10:22	arg1	synthase					42:49	a glucomannan synthase	28:49	a glucomannan synthase	28:49	CELLULOSE SYNTHASE-LIKE A2, a glucomannan synthase, is involved in maintaining adherent mucilage structure in Arabidopsis seed.
24569843	11	27	theme	cellulose	1786:1794	arg1	organization					1796:1807	cellulose organization	1786:1807	cellulose organization	1786:1807	Taken together, our results demonstrate that glucomannans synthesized by CSLA2 are involved in modulating the structure of adherent mucilage, potentially through altering cellulose organization and crystallization.
24569843	10	28	theme	spatial	1545:1551	arg1	distribution					1553:1564	the spatial distribution	1541:1564	the spatial distribution of pectin	1541:1574	As with the observed changes in crystalline cellulose, the spatial distribution of pectin was also modified in csla2-1 mucilage.
24569843	3	29	theme	A2	403:404	arg1	expression					425:434	CELLULOSE SYNTHASE-LIKE A2 (CSLA2; At5g22740) expression	379:434	CELLULOSE SYNTHASE-LIKE A2 (CSLA2; At5g22740) expression	379:434	In this study, CELLULOSE SYNTHASE-LIKE A2 (CSLA2; At5g22740) expression was observed in several seed tissues, including the epidermal cells of developing seed coats.
24569843	0	30	theme	CELLULOSE	0:8	arg1	A2					24:25	CELLULOSE SYNTHASE-LIKE A2	0:25	CELLULOSE SYNTHASE-LIKE A2	0:25	CELLULOSE SYNTHASE-LIKE A2, a glucomannan synthase, is involved in maintaining adherent mucilage structure in Arabidopsis seed.
24569843	0	30	theme	CELLULOSE	0:8	arg1	synthase					42:49	a glucomannan synthase	28:49	a glucomannan synthase	28:49	CELLULOSE SYNTHASE-LIKE A2, a glucomannan synthase, is involved in maintaining adherent mucilage structure in Arabidopsis seed.
24569843	6	31	theme	mannosyl	899:906	arg1	%					892:892	30%	890:892	30% less mannosyl	890:906	In accordance with the role of CSLA2 in glucomannan synthesis, csla2-1 mucilage contained 30% less mannosyl and glucosyl content than did the wild type.
24569843	8	32	with	treatment	1275:1283	arg1	endo-β-glucanase					1307:1322	endo-β-glucanase	1307:1322	endo-β-glucanase	1307:1322	Biochemical analysis revealed that cellulose crystallinity was substantially reduced in csla2-1 mucilage; this was supported by the removal of most mucilage cellulose through treatment of csla2-1 seeds with endo-β-glucanase.
24569843	10	33	theme	pectin	1569:1574	arg1	distribution					1553:1564	the spatial distribution	1541:1564	the spatial distribution of pectin	1541:1574	As with the observed changes in crystalline cellulose, the spatial distribution of pectin was also modified in csla2-1 mucilage.
24569843	8	34	theme	mucilage	1248:1255	arg1	cellulose					1257:1265	most mucilage cellulose	1243:1265	most mucilage cellulose	1243:1265	Biochemical analysis revealed that cellulose crystallinity was substantially reduced in csla2-1 mucilage; this was supported by the removal of most mucilage cellulose through treatment of csla2-1 seeds with endo-β-glucanase.
24569843	3	35	theme	seed	518:521	arg1	coats					523:527	developing seed coats	507:527	developing seed coats	507:527	In this study, CELLULOSE SYNTHASE-LIKE A2 (CSLA2; At5g22740) expression was observed in several seed tissues, including the epidermal cells of developing seed coats.
24569843	9	36	theme	birefringent	1420:1431	arg1	microfibrils					1443:1454	birefringent cellulose microfibrils	1420:1454	birefringent cellulose microfibrils	1420:1454	Mutation in CSLA2 also resulted in altered spatial distribution of cellulose and an absence of birefringent cellulose microfibrils within the adherent mucilage.
24569843	7	37	from	changes	968:974	arg1	structure					996:1004	structure	996:1004	structure	996:1004	No appreciable changes in the composition, structure, or macromolecular properties were observed for nonmannan polysaccharides in mutant mucilage.
24569843	7	37	from	changes	968:974	arg1	composition					983:993	the composition	979:993	the composition	979:993	No appreciable changes in the composition, structure, or macromolecular properties were observed for nonmannan polysaccharides in mutant mucilage.
24569843	7	37	from	changes	968:974	arg1	properties					1025:1034	macromolecular properties	1010:1034	macromolecular properties	1010:1034	No appreciable changes in the composition, structure, or macromolecular properties were observed for nonmannan polysaccharides in mutant mucilage.
24569843	11	38	theme	adherent	1738:1745	arg1	mucilage					1747:1754	adherent mucilage	1738:1754	adherent mucilage	1738:1754	Taken together, our results demonstrate that glucomannans synthesized by CSLA2 are involved in modulating the structure of adherent mucilage, potentially through altering cellulose organization and crystallization.
24569843	0	39	theme	Arabidopsis	110:120	arg1	seed					122:125	Arabidopsis seed	110:125	Arabidopsis seed	110:125	CELLULOSE SYNTHASE-LIKE A2, a glucomannan synthase, is involved in maintaining adherent mucilage structure in Arabidopsis seed.
24569843	1	40	contain	have	194:197	arg2	functions					227:235	both structural and storage functions	199:235	both structural and storage functions	199:235	Mannans are hemicellulosic polysaccharides that are considered to have both structural and storage functions in the plant cell wall.
24569843	1	40	contain	have	194:197	arg1	Mannans					128:134	Mannans	128:134	Mannans	128:134	Mannans are hemicellulosic polysaccharides that are considered to have both structural and storage functions in the plant cell wall.
24569843	1	40	contain	have	194:197	arg1	polysaccharides					155:169	hemicellulosic polysaccharides	140:169	hemicellulosic polysaccharides that are considered to have both structural and storage functions in the plant cell wall	140:258	Mannans are hemicellulosic polysaccharides that are considered to have both structural and storage functions in the plant cell wall.
24569843	3	41	theme	coats	523:527	arg1	cells					498:502	the epidermal cells	484:502	the epidermal cells of developing seed coats	484:527	In this study, CELLULOSE SYNTHASE-LIKE A2 (CSLA2; At5g22740) expression was observed in several seed tissues, including the epidermal cells of developing seed coats.
24569843	9	42	theme	cellulose	1392:1400	arg1	absence					1409:1415	an absence	1406:1415	an absence of birefringent cellulose microfibrils	1406:1454	Mutation in CSLA2 also resulted in altered spatial distribution of cellulose and an absence of birefringent cellulose microfibrils within the adherent mucilage.
24569843	9	42	theme	cellulose	1392:1400	arg1	distribution					1376:1387	altered spatial distribution	1360:1387	altered spatial distribution of cellulose	1360:1400	Mutation in CSLA2 also resulted in altered spatial distribution of cellulose and an absence of birefringent cellulose microfibrils within the adherent mucilage.
24569843	1	43	theme	structural	204:213	arg1	functions					227:235	both structural and storage functions	199:235	both structural and storage functions	199:235	Mannans are hemicellulosic polysaccharides that are considered to have both structural and storage functions in the plant cell wall.
24569843	3	44	theme	CELLULOSE	379:387	arg1	CSLA2					407:411	CSLA2	407:411	CSLA2; At5g22740	407:422	In this study, CELLULOSE SYNTHASE-LIKE A2 (CSLA2; At5g22740) expression was observed in several seed tissues, including the epidermal cells of developing seed coats.
24569843	3	44	theme	CELLULOSE	379:387	arg1	A2					403:404	CELLULOSE SYNTHASE-LIKE A2	379:404	CELLULOSE SYNTHASE-LIKE A2 (CSLA2; At5g22740) expression	379:434	In this study, CELLULOSE SYNTHASE-LIKE A2 (CSLA2; At5g22740) expression was observed in several seed tissues, including the epidermal cells of developing seed coats.
24569843	3	45	theme	seed	460:463	arg1	cells					498:502	the epidermal cells	484:502	the epidermal cells of developing seed coats	484:527	In this study, CELLULOSE SYNTHASE-LIKE A2 (CSLA2; At5g22740) expression was observed in several seed tissues, including the epidermal cells of developing seed coats.
24569843	3	45	theme	seed	460:463	arg1	tissues					465:471	several seed tissues	452:471	several seed tissues	452:471	In this study, CELLULOSE SYNTHASE-LIKE A2 (CSLA2; At5g22740) expression was observed in several seed tissues, including the epidermal cells of developing seed coats.
24569843	9	46	theme	adherent	1467:1474	arg1	mucilage					1476:1483	the adherent mucilage	1463:1483	the adherent mucilage	1463:1483	Mutation in CSLA2 also resulted in altered spatial distribution of cellulose and an absence of birefringent cellulose microfibrils within the adherent mucilage.
24569843	9	47	theme	microfibrils	1443:1454	arg1	absence					1409:1415	an absence	1406:1415	an absence of birefringent cellulose microfibrils	1406:1454	Mutation in CSLA2 also resulted in altered spatial distribution of cellulose and an absence of birefringent cellulose microfibrils within the adherent mucilage.
24569843	9	47	theme	microfibrils	1443:1454	arg1	distribution					1376:1387	altered spatial distribution	1360:1387	altered spatial distribution of cellulose	1360:1400	Mutation in CSLA2 also resulted in altered spatial distribution of cellulose and an absence of birefringent cellulose microfibrils within the adherent mucilage.
24569843	8	48	theme	cellulose	1135:1143	arg1	crystallinity					1145:1157	cellulose crystallinity	1135:1157	cellulose crystallinity	1135:1157	Biochemical analysis revealed that cellulose crystallinity was substantially reduced in csla2-1 mucilage; this was supported by the removal of most mucilage cellulose through treatment of csla2-1 seeds with endo-β-glucanase.
24569843	4	49	theme	total	608:612	arg1	mucilage					637:644	the adherent mucilage	624:644	the adherent mucilage	624:644	Disruption of CSLA2 resulted in thinner adherent mucilage halos, although the total amount of the adherent mucilage did not change compared with the wild type.
24569843	4	49	theme	total	608:612	arg1	amount					614:619	the total amount	604:619	the total amount of the adherent mucilage	604:644	Disruption of CSLA2 resulted in thinner adherent mucilage halos, although the total amount of the adherent mucilage did not change compared with the wild type.
24569843	3	50	theme	SYNTHASE-LIKE	389:401	arg1	CSLA2					407:411	CSLA2	407:411	CSLA2; At5g22740	407:422	In this study, CELLULOSE SYNTHASE-LIKE A2 (CSLA2; At5g22740) expression was observed in several seed tissues, including the epidermal cells of developing seed coats.
24569843	3	50	theme	SYNTHASE-LIKE	389:401	arg1	A2					403:404	CELLULOSE SYNTHASE-LIKE A2	379:404	CELLULOSE SYNTHASE-LIKE A2 (CSLA2; At5g22740) expression	379:434	In this study, CELLULOSE SYNTHASE-LIKE A2 (CSLA2; At5g22740) expression was observed in several seed tissues, including the epidermal cells of developing seed coats.
24569843	7	51	theme	mutant	1083:1088	arg1	mucilage					1090:1097	mutant mucilage	1083:1097	mutant mucilage	1083:1097	No appreciable changes in the composition, structure, or macromolecular properties were observed for nonmannan polysaccharides in mutant mucilage.
24569843	8	52	theme	cellulose	1257:1265	arg1	removal					1232:1238	the removal	1228:1238	the removal of most mucilage cellulose through treatment of csla2-1 seeds with endo-β-glucanase	1228:1322	Biochemical analysis revealed that cellulose crystallinity was substantially reduced in csla2-1 mucilage; this was supported by the removal of most mucilage cellulose through treatment of csla2-1 seeds with endo-β-glucanase.
24569843	9	53	theme	spatial	1368:1374	arg1	distribution					1376:1387	altered spatial distribution	1360:1387	altered spatial distribution of cellulose	1360:1400	Mutation in CSLA2 also resulted in altered spatial distribution of cellulose and an absence of birefringent cellulose microfibrils within the adherent mucilage.
24569843	1	54	theme	storage	219:225	arg1	functions					227:235	both structural and storage functions	199:235	both structural and storage functions	199:235	Mannans are hemicellulosic polysaccharides that are considered to have both structural and storage functions in the plant cell wall.
24569843	7	55	theme	nonmannan	1054:1062	arg1	polysaccharides					1064:1078	nonmannan polysaccharides	1054:1078	nonmannan polysaccharides in mutant mucilage	1054:1097	No appreciable changes in the composition, structure, or macromolecular properties were observed for nonmannan polysaccharides in mutant mucilage.
24569843	10	56	mod	modified	1585:1592	arg3	changes					1507:1513	the observed changes	1494:1513	the observed changes in crystalline cellulose	1494:1538	As with the observed changes in crystalline cellulose, the spatial distribution of pectin was also modified in csla2-1 mucilage.
24569843	10	56	mod	modified	1585:1592	arg1	distribution					1553:1564	the spatial distribution	1541:1564	the spatial distribution of pectin	1541:1574	As with the observed changes in crystalline cellulose, the spatial distribution of pectin was also modified in csla2-1 mucilage.
24569843	7	57	theme	appreciable	956:966	arg1	changes					968:974	No appreciable changes	953:974	No appreciable changes in the composition, structure, or macromolecular properties	953:1034	No appreciable changes in the composition, structure, or macromolecular properties were observed for nonmannan polysaccharides in mutant mucilage.
24569843	3	58	dep	CSLA2	407:411	arg1	At5g22740					414:422	At5g22740	414:422	CSLA2; At5g22740	407:422	In this study, CELLULOSE SYNTHASE-LIKE A2 (CSLA2; At5g22740) expression was observed in several seed tissues, including the epidermal cells of developing seed coats.
24569843	11	59	theme	mucilage	1747:1754	arg1	structure					1725:1733	the structure	1721:1733	the structure of adherent mucilage	1721:1754	Taken together, our results demonstrate that glucomannans synthesized by CSLA2 are involved in modulating the structure of adherent mucilage, potentially through altering cellulose organization and crystallization.
24569843	3	60	theme	several	452:458	arg1	cells					498:502	the epidermal cells	484:502	the epidermal cells of developing seed coats	484:527	In this study, CELLULOSE SYNTHASE-LIKE A2 (CSLA2; At5g22740) expression was observed in several seed tissues, including the epidermal cells of developing seed coats.
24569843	3	60	theme	several	452:458	arg1	tissues					465:471	several seed tissues	452:471	several seed tissues	452:471	In this study, CELLULOSE SYNTHASE-LIKE A2 (CSLA2; At5g22740) expression was observed in several seed tissues, including the epidermal cells of developing seed coats.
24569843	6	61	theme	csla2-1	863:869	arg1	mucilage					871:878	csla2-1 mucilage	863:878	csla2-1 mucilage	863:878	In accordance with the role of CSLA2 in glucomannan synthesis, csla2-1 mucilage contained 30% less mannosyl and glucosyl content than did the wild type.
24569843	7	62	theme	macromolecular	1010:1023	arg1	properties					1025:1034	macromolecular properties	1010:1034	macromolecular properties	1010:1034	No appreciable changes in the composition, structure, or macromolecular properties were observed for nonmannan polysaccharides in mutant mucilage.
24569843	2	63	theme	Arabidopsis	314:324	arg1	thaliana					339:346	Arabidopsis thaliana	327:346	Arabidopsis thaliana	327:346	However, it is not yet known how mannans function in Arabidopsis (Arabidopsis thaliana) seed mucilage.
24569843	2	63	theme	Arabidopsis	314:324	arg1	mucilage					354:361	Arabidopsis (Arabidopsis thaliana) seed mucilage	314:361	Arabidopsis (Arabidopsis thaliana) seed mucilage	314:361	However, it is not yet known how mannans function in Arabidopsis (Arabidopsis thaliana) seed mucilage.
24569843	6	64	contain	contained	880:888	arg1	mucilage					871:878	csla2-1 mucilage	863:878	csla2-1 mucilage	863:878	In accordance with the role of CSLA2 in glucomannan synthesis, csla2-1 mucilage contained 30% less mannosyl and glucosyl content than did the wild type.
24569843	6	64	contain	contained	880:888	arg2	%					892:892	30%	890:892	30% less mannosyl	890:906	In accordance with the role of CSLA2 in glucomannan synthesis, csla2-1 mucilage contained 30% less mannosyl and glucosyl content than did the wild type.
24569843	6	64	contain	contained	880:888	arg2	content					921:927	glucosyl content	912:927	glucosyl content	912:927	In accordance with the role of CSLA2 in glucomannan synthesis, csla2-1 mucilage contained 30% less mannosyl and glucosyl content than did the wild type.
24569843	5	65	from	mucilage	723:730	arg1	mutant					739:744	the mutant	735:744	the mutant	735:744	This suggested that the adherent mucilage in the mutant was more compact compared with that of the wild type.
24569843	4	66	theme	wild	679:682	arg1	type					684:687	the wild type	675:687	the wild type	675:687	Disruption of CSLA2 resulted in thinner adherent mucilage halos, although the total amount of the adherent mucilage did not change compared with the wild type.
24569843	4	67	theme	adherent	570:577	arg1	halos					588:592	thinner adherent mucilage halos	562:592	thinner adherent mucilage halos	562:592	Disruption of CSLA2 resulted in thinner adherent mucilage halos, although the total amount of the adherent mucilage did not change compared with the wild type.
24569843	4	68	theme	CSLA2	544:548	arg1	Disruption					530:539	Disruption	530:539	Disruption of CSLA2	530:548	Disruption of CSLA2 resulted in thinner adherent mucilage halos, although the total amount of the adherent mucilage did not change compared with the wild type.
24569843	4	69	theme	mucilage	637:644	arg1	mucilage					637:644	the adherent mucilage	624:644	the adherent mucilage	624:644	Disruption of CSLA2 resulted in thinner adherent mucilage halos, although the total amount of the adherent mucilage did not change compared with the wild type.
24569843	4	69	theme	mucilage	637:644	arg1	amount					614:619	the total amount	604:619	the total amount of the adherent mucilage	604:644	Disruption of CSLA2 resulted in thinner adherent mucilage halos, although the total amount of the adherent mucilage did not change compared with the wild type.
24569843	4	70	theme	thinner	562:568	arg1	halos					588:592	thinner adherent mucilage halos	562:592	thinner adherent mucilage halos	562:592	Disruption of CSLA2 resulted in thinner adherent mucilage halos, although the total amount of the adherent mucilage did not change compared with the wild type.
24569843	10	71	theme	csla2-1	1597:1603	arg1	mucilage					1605:1612	csla2-1 mucilage	1597:1612	csla2-1 mucilage	1597:1612	As with the observed changes in crystalline cellulose, the spatial distribution of pectin was also modified in csla2-1 mucilage.
24569843	5	72	theme	wild	789:792	arg1	type					794:797	the wild type	785:797	the wild type	785:797	This suggested that the adherent mucilage in the mutant was more compact compared with that of the wild type.
24569843	2	73	theme	seed	349:352	arg1	thaliana					339:346	Arabidopsis thaliana	327:346	Arabidopsis thaliana	327:346	However, it is not yet known how mannans function in Arabidopsis (Arabidopsis thaliana) seed mucilage.
24569843	2	73	theme	seed	349:352	arg1	mucilage					354:361	Arabidopsis (Arabidopsis thaliana) seed mucilage	314:361	Arabidopsis (Arabidopsis thaliana) seed mucilage	314:361	However, it is not yet known how mannans function in Arabidopsis (Arabidopsis thaliana) seed mucilage.
24569843	10	74	theme	crystalline	1518:1528	arg1	cellulose					1530:1538	crystalline cellulose	1518:1538	crystalline cellulose	1518:1538	As with the observed changes in crystalline cellulose, the spatial distribution of pectin was also modified in csla2-1 mucilage.
24569843	4	75	theme	mucilage	579:586	arg1	halos					588:592	thinner adherent mucilage halos	562:592	thinner adherent mucilage halos	562:592	Disruption of CSLA2 resulted in thinner adherent mucilage halos, although the total amount of the adherent mucilage did not change compared with the wild type.
24569843	1	76	theme	hemicellulosic	140:153	arg1	Mannans					128:134	Mannans	128:134	Mannans	128:134	Mannans are hemicellulosic polysaccharides that are considered to have both structural and storage functions in the plant cell wall.
24569843	1	76	theme	hemicellulosic	140:153	arg1	polysaccharides					155:169	hemicellulosic polysaccharides	140:169	hemicellulosic polysaccharides that are considered to have both structural and storage functions in the plant cell wall	140:258	Mannans are hemicellulosic polysaccharides that are considered to have both structural and storage functions in the plant cell wall.
24569843	9	77	theme	altered	1360:1366	arg1	distribution					1376:1387	altered spatial distribution	1360:1387	altered spatial distribution of cellulose	1360:1400	Mutation in CSLA2 also resulted in altered spatial distribution of cellulose and an absence of birefringent cellulose microfibrils within the adherent mucilage.
24569843	1	78	theme	plant	244:248	arg1	wall					255:258	the plant cell wall	240:258	the plant cell wall	240:258	Mannans are hemicellulosic polysaccharides that are considered to have both structural and storage functions in the plant cell wall.
27612713	0	0	theme	improved	68:75	arg1	structure					85:93	improved network structure	68:93	improved network structure	68:93	The composite hydrogels of polyvinyl alcohol-gellan gum-Ca(2+) with improved network structure and mechanical property.
27612713	1	1	theme	polyvinyl	147:155	arg1	PVA					166:168	PVA	166:168	PVA	166:168	The composite hydrogels of polyvinyl alcohol (PVA) and gellan gum (GG) are of interesting in the biomaterials application.
27612713	1	1	theme	polyvinyl	147:155	arg1	alcohol					157:163	polyvinyl alcohol	147:163	polyvinyl alcohol (PVA)	147:169	The composite hydrogels of polyvinyl alcohol (PVA) and gellan gum (GG) are of interesting in the biomaterials application.
27612713	1	2	theme	alcohol	157:163	arg1	hydrogels					134:142	The composite hydrogels	120:142	The composite hydrogels of polyvinyl alcohol (PVA) and gellan gum (GG)	120:189	The composite hydrogels of polyvinyl alcohol (PVA) and gellan gum (GG) are of interesting in the biomaterials application.
27612713	5	3	dep	biomaterials	920:931	arg1	application					933:943	application	933:943	biomaterials application	920:943	These observations will benefit the development of hydrogels in biomaterials application.
27612713	4	4	theme	bonding	807:813	arg1	interactions					815:826	the electrolytic and hydrogen bonding interactions	777:826	interactions	815:826	Our result indicates that, as GG is added to hydrogel network, the role of Ca(2+) ion is stand out, it reorganizes the network structure, enhances the mechanical properties, and strengthens the electrolytic and hydrogen bonding interactions in PVA-GG-Ca(2+) hydrogels.
27612713	0	5	theme	network	77:83	arg1	structure					85:93	improved network structure	68:93	improved network structure	68:93	The composite hydrogels of polyvinyl alcohol-gellan gum-Ca(2+) with improved network structure and mechanical property.
27612713	2	6	theme	Ca	307:308	arg1	ion					314:316	Ca(2+) ion	307:316	Ca(2+) ion	307:316	To improve the structure and mechanical property, in this work, Ca(2+) ion was introduced to crosslink the polymer chain, and the PVA-GG-Ca(2+) hydrogel was formed.
27612713	0	7	theme	mechanical	99:108	arg1	property					110:117	mechanical property	99:117	mechanical property	99:117	The composite hydrogels of polyvinyl alcohol-gellan gum-Ca(2+) with improved network structure and mechanical property.
27612713	4	8	theme	hydrogel	632:639	arg1	network					641:647	hydrogel network	632:647	hydrogel network	632:647	Our result indicates that, as GG is added to hydrogel network, the role of Ca(2+) ion is stand out, it reorganizes the network structure, enhances the mechanical properties, and strengthens the electrolytic and hydrogen bonding interactions in PVA-GG-Ca(2+) hydrogels.
27612713	4	9	theme	Ca	662:663	arg1	ion					669:671	Ca(2+) ion	662:671	Ca(2+) ion	662:671	Our result indicates that, as GG is added to hydrogel network, the role of Ca(2+) ion is stand out, it reorganizes the network structure, enhances the mechanical properties, and strengthens the electrolytic and hydrogen bonding interactions in PVA-GG-Ca(2+) hydrogels.
27612713	0	10	theme	gum-Ca	52:57	arg1	hydrogels					14:22	The composite hydrogels	0:22	The composite hydrogels of polyvinyl alcohol-gellan gum-Ca(2+) with improved network structure and mechanical property.	0:118	The composite hydrogels of polyvinyl alcohol-gellan gum-Ca(2+) with improved network structure and mechanical property.
27612713	3	11	theme	mechanical	436:445	arg1	properties					447:456	mechanical properties	436:456	mechanical properties	436:456	By analyzing its structure, mechanical properties, swelling and dehydration kinetics, the effect of molecular interaction on hydrogel structure and properties have been observed.
27612713	3	11	theme	mechanical	436:445	arg1	effect					498:503	the effect	494:503	the effect of molecular interaction on hydrogel structure and properties	494:565	By analyzing its structure, mechanical properties, swelling and dehydration kinetics, the effect of molecular interaction on hydrogel structure and properties have been observed.
27612713	3	11	theme	mechanical	436:445	arg1	kinetics					484:491	swelling and dehydration kinetics	459:491	swelling and dehydration kinetics	459:491	By analyzing its structure, mechanical properties, swelling and dehydration kinetics, the effect of molecular interaction on hydrogel structure and properties have been observed.
27612713	0	12	theme	composite	4:12	arg1	hydrogels					14:22	The composite hydrogels	0:22	The composite hydrogels of polyvinyl alcohol-gellan gum-Ca(2+) with improved network structure and mechanical property.	0:118	The composite hydrogels of polyvinyl alcohol-gellan gum-Ca(2+) with improved network structure and mechanical property.
27612713	4	13	theme	network	706:712	arg1	structure					714:722	the network structure	702:722	the network structure	702:722	Our result indicates that, as GG is added to hydrogel network, the role of Ca(2+) ion is stand out, it reorganizes the network structure, enhances the mechanical properties, and strengthens the electrolytic and hydrogen bonding interactions in PVA-GG-Ca(2+) hydrogels.
27612713	3	14	theme	dehydration	472:482	arg1	properties					447:456	mechanical properties	436:456	mechanical properties	436:456	By analyzing its structure, mechanical properties, swelling and dehydration kinetics, the effect of molecular interaction on hydrogel structure and properties have been observed.
27612713	3	14	theme	dehydration	472:482	arg1	kinetics					484:491	swelling and dehydration kinetics	459:491	swelling and dehydration kinetics	459:491	By analyzing its structure, mechanical properties, swelling and dehydration kinetics, the effect of molecular interaction on hydrogel structure and properties have been observed.
27612713	4	15	theme	mechanical	738:747	arg1	properties					749:758	the mechanical properties	734:758	the mechanical properties	734:758	Our result indicates that, as GG is added to hydrogel network, the role of Ca(2+) ion is stand out, it reorganizes the network structure, enhances the mechanical properties, and strengthens the electrolytic and hydrogen bonding interactions in PVA-GG-Ca(2+) hydrogels.
27612713	2	16	theme	mechanical	272:281	arg1	property					283:290	mechanical property	272:290	mechanical property	272:290	To improve the structure and mechanical property, in this work, Ca(2+) ion was introduced to crosslink the polymer chain, and the PVA-GG-Ca(2+) hydrogel was formed.
27612713	4	17	theme	ion	669:671	arg1	role					654:657	the role	650:657	the role of Ca(2+) ion	650:671	Our result indicates that, as GG is added to hydrogel network, the role of Ca(2+) ion is stand out, it reorganizes the network structure, enhances the mechanical properties, and strengthens the electrolytic and hydrogen bonding interactions in PVA-GG-Ca(2+) hydrogels.
27612713	4	17	theme	ion	669:671	arg1	stand					676:680	stand	676:680	stand	676:680	Our result indicates that, as GG is added to hydrogel network, the role of Ca(2+) ion is stand out, it reorganizes the network structure, enhances the mechanical properties, and strengthens the electrolytic and hydrogen bonding interactions in PVA-GG-Ca(2+) hydrogels.
27612713	1	18	from	biomaterials	217:228	arg1	interesting					198:208	interesting	198:208	interesting	198:208	The composite hydrogels of polyvinyl alcohol (PVA) and gellan gum (GG) are of interesting in the biomaterials application.
27612713	3	19	theme	swelling	459:466	arg1	properties					447:456	mechanical properties	436:456	mechanical properties	436:456	By analyzing its structure, mechanical properties, swelling and dehydration kinetics, the effect of molecular interaction on hydrogel structure and properties have been observed.
27612713	3	19	theme	swelling	459:466	arg1	kinetics					484:491	swelling and dehydration kinetics	459:491	swelling and dehydration kinetics	459:491	By analyzing its structure, mechanical properties, swelling and dehydration kinetics, the effect of molecular interaction on hydrogel structure and properties have been observed.
27612713	5	20	from	development	892:902	arg1	biomaterials					920:931	biomaterials application	920:943	biomaterials application	920:943	These observations will benefit the development of hydrogels in biomaterials application.
27612713	2	21	theme	polymer	350:356	arg1	chain					358:362	the polymer chain	346:362	the polymer chain	346:362	To improve the structure and mechanical property, in this work, Ca(2+) ion was introduced to crosslink the polymer chain, and the PVA-GG-Ca(2+) hydrogel was formed.
27612713	3	22	from	effect	498:503	arg1	properties					556:565	properties	556:565	properties	556:565	By analyzing its structure, mechanical properties, swelling and dehydration kinetics, the effect of molecular interaction on hydrogel structure and properties have been observed.
27612713	3	22	from	effect	498:503	arg1	structure					542:550	hydrogel structure	533:550	hydrogel structure	533:550	By analyzing its structure, mechanical properties, swelling and dehydration kinetics, the effect of molecular interaction on hydrogel structure and properties have been observed.
27612713	1	23	theme	gum	182:184	arg1	hydrogels					134:142	The composite hydrogels	120:142	The composite hydrogels of polyvinyl alcohol (PVA) and gellan gum (GG)	120:189	The composite hydrogels of polyvinyl alcohol (PVA) and gellan gum (GG) are of interesting in the biomaterials application.
27612713	0	24	theme	alcohol-gellan	37:50	arg1	2+					59:60	2+	59:60	2+	59:60	The composite hydrogels of polyvinyl alcohol-gellan gum-Ca(2+) with improved network structure and mechanical property.
27612713	0	24	theme	alcohol-gellan	37:50	arg1	gum-Ca					52:57	polyvinyl alcohol-gellan gum-Ca	27:57	polyvinyl alcohol-gellan gum-Ca(2+)	27:61	The composite hydrogels of polyvinyl alcohol-gellan gum-Ca(2+) with improved network structure and mechanical property.
27612713	4	25	theme	hydrogen	798:805	arg1	interactions					815:826	the electrolytic and hydrogen bonding interactions	777:826	interactions	815:826	Our result indicates that, as GG is added to hydrogel network, the role of Ca(2+) ion is stand out, it reorganizes the network structure, enhances the mechanical properties, and strengthens the electrolytic and hydrogen bonding interactions in PVA-GG-Ca(2+) hydrogels.
27612713	4	26	from	electrolytic	781:792	arg1	hydrogels					845:853	PVA-GG-Ca(2+) hydrogels	831:853	PVA-GG-Ca(2+) hydrogels	831:853	Our result indicates that, as GG is added to hydrogel network, the role of Ca(2+) ion is stand out, it reorganizes the network structure, enhances the mechanical properties, and strengthens the electrolytic and hydrogen bonding interactions in PVA-GG-Ca(2+) hydrogels.
27612713	2	27	theme	2+	383:384	arg1	hydrogel					387:394	the PVA-GG-Ca(2+) hydrogel	369:394	the PVA-GG-Ca(2+) hydrogel	369:394	To improve the structure and mechanical property, in this work, Ca(2+) ion was introduced to crosslink the polymer chain, and the PVA-GG-Ca(2+) hydrogel was formed.
27612713	0	28	theme	polyvinyl	27:35	arg1	2+					59:60	2+	59:60	2+	59:60	The composite hydrogels of polyvinyl alcohol-gellan gum-Ca(2+) with improved network structure and mechanical property.
27612713	0	28	theme	polyvinyl	27:35	arg1	gum-Ca					52:57	polyvinyl alcohol-gellan gum-Ca	27:57	polyvinyl alcohol-gellan gum-Ca(2+)	27:61	The composite hydrogels of polyvinyl alcohol-gellan gum-Ca(2+) with improved network structure and mechanical property.
27612713	0	29	with	hydrogels	14:22	arg1	structure					85:93	improved network structure	68:93	improved network structure	68:93	The composite hydrogels of polyvinyl alcohol-gellan gum-Ca(2+) with improved network structure and mechanical property.
27612713	0	29	with	hydrogels	14:22	arg1	property					110:117	mechanical property	99:117	mechanical property	99:117	The composite hydrogels of polyvinyl alcohol-gellan gum-Ca(2+) with improved network structure and mechanical property.
27612713	2	30	theme	PVA-GG-Ca	373:381	arg1	hydrogel					387:394	the PVA-GG-Ca(2+) hydrogel	369:394	the PVA-GG-Ca(2+) hydrogel	369:394	To improve the structure and mechanical property, in this work, Ca(2+) ion was introduced to crosslink the polymer chain, and the PVA-GG-Ca(2+) hydrogel was formed.
27612713	1	31	from	interesting	198:208	arg1	biomaterials					217:228	the biomaterials	213:228	the biomaterials	213:228	The composite hydrogels of polyvinyl alcohol (PVA) and gellan gum (GG) are of interesting in the biomaterials application.
27612713	4	32	from	hydrogels	845:853	arg1	electrolytic					781:792	electrolytic	781:792	electrolytic	781:792	Our result indicates that, as GG is added to hydrogel network, the role of Ca(2+) ion is stand out, it reorganizes the network structure, enhances the mechanical properties, and strengthens the electrolytic and hydrogen bonding interactions in PVA-GG-Ca(2+) hydrogels.
27612713	5	33	theme	hydrogels	907:915	arg1	development					892:902	the development	888:902	the development of hydrogels in biomaterials application	888:943	These observations will benefit the development of hydrogels in biomaterials application.
27612713	4	34	theme	2+	665:666	arg1	ion					669:671	Ca(2+) ion	662:671	Ca(2+) ion	662:671	Our result indicates that, as GG is added to hydrogel network, the role of Ca(2+) ion is stand out, it reorganizes the network structure, enhances the mechanical properties, and strengthens the electrolytic and hydrogen bonding interactions in PVA-GG-Ca(2+) hydrogels.
27612713	3	35	theme	hydrogel	533:540	arg1	structure					542:550	hydrogel structure	533:550	hydrogel structure	533:550	By analyzing its structure, mechanical properties, swelling and dehydration kinetics, the effect of molecular interaction on hydrogel structure and properties have been observed.
27612713	1	36	theme	composite	124:132	arg1	hydrogels					134:142	The composite hydrogels	120:142	The composite hydrogels of polyvinyl alcohol (PVA) and gellan gum (GG)	120:189	The composite hydrogels of polyvinyl alcohol (PVA) and gellan gum (GG) are of interesting in the biomaterials application.
27612713	3	37	theme	molecular	508:516	arg1	interaction					518:528	molecular interaction	508:528	molecular interaction	508:528	By analyzing its structure, mechanical properties, swelling and dehydration kinetics, the effect of molecular interaction on hydrogel structure and properties have been observed.
27612713	1	38	theme	gellan	175:180	arg1	gum					182:184	gellan gum	175:184	gellan gum (GG)	175:189	The composite hydrogels of polyvinyl alcohol (PVA) and gellan gum (GG) are of interesting in the biomaterials application.
27612713	1	38	theme	gellan	175:180	arg1	GG					187:188	GG	187:188	GG	187:188	The composite hydrogels of polyvinyl alcohol (PVA) and gellan gum (GG) are of interesting in the biomaterials application.
27612713	4	39	theme	PVA-GG-Ca	831:839	arg1	hydrogels					845:853	PVA-GG-Ca(2+) hydrogels	831:853	PVA-GG-Ca(2+) hydrogels	831:853	Our result indicates that, as GG is added to hydrogel network, the role of Ca(2+) ion is stand out, it reorganizes the network structure, enhances the mechanical properties, and strengthens the electrolytic and hydrogen bonding interactions in PVA-GG-Ca(2+) hydrogels.
27612713	3	40	theme	interaction	518:528	arg1	effect					498:503	the effect	494:503	the effect of molecular interaction on hydrogel structure and properties	494:565	By analyzing its structure, mechanical properties, swelling and dehydration kinetics, the effect of molecular interaction on hydrogel structure and properties have been observed.
27612713	3	40	theme	interaction	518:528	arg1	properties					447:456	mechanical properties	436:456	mechanical properties	436:456	By analyzing its structure, mechanical properties, swelling and dehydration kinetics, the effect of molecular interaction on hydrogel structure and properties have been observed.
27612713	1	41	theme	interesting	198:208	arg1	application					230:240	interesting in the biomaterials application	198:240	interesting in the biomaterials application	198:240	The composite hydrogels of polyvinyl alcohol (PVA) and gellan gum (GG) are of interesting in the biomaterials application.
25064557	3	0	theme	cell	547:550	arg1	proliferation					552:564	cell proliferation	547:564	cell proliferation	547:564	Cell adhesion was determined quantitatively by LDH total test and cell proliferation was assessed by confocal microscope observation.
25064557	3	1	theme	confocal	582:589	arg1	observation					602:612	confocal microscope observation	582:612	confocal microscope observation	582:612	Cell adhesion was determined quantitatively by LDH total test and cell proliferation was assessed by confocal microscope observation.
25064557	2	2	dep	evaluated	365:373	arg1	using					386:390	using	386:390	using fluid extracts	386:405	Cytotoxicity of materials was evaluated indirectly using fluid extracts and by direct-contact method using live/dead double fluorescent staining.
25064557	2	2	dep	evaluated	365:373	arg1	method					429:434	direct-contact method	414:434	direct-contact method using live/dead double fluorescent staining	414:478	Cytotoxicity of materials was evaluated indirectly using fluid extracts and by direct-contact method using live/dead double fluorescent staining.
25064557	3	3	theme	microscope	591:600	arg1	observation					602:612	confocal microscope observation	582:612	confocal microscope observation	582:612	Cell adhesion was determined quantitatively by LDH total test and cell proliferation was assessed by confocal microscope observation.
25064557	6	4	contain	containing	987:996	arg2	1,3-β-D-glucan					998:1011	1,3-β-D-glucan	998:1011	1,3-β-D-glucan	998:1011	However, significantly better osteoblast growth was observed on scaffolds containing 1,3-β-D-glucan.
25064557	6	4	contain	containing	987:996	arg1	scaffolds					977:985	scaffolds	977:985	scaffolds containing 1,3-β-D-glucan	977:1011	However, significantly better osteoblast growth was observed on scaffolds containing 1,3-β-D-glucan.
25064557	4	5	theme	cells	798:802	arg1	proliferation					770:782	proliferation	770:782	proliferation	770:782	Obtained data clearly prove that addition of 1,3-β-D-glucan to the bi-component chitosan/bioceramic materials significantly enhances adhesion, growth, and proliferation of osteoblast cells.
25064557	4	5	theme	cells	798:802	arg1	adhesion					748:755	adhesion	748:755	adhesion	748:755	Obtained data clearly prove that addition of 1,3-β-D-glucan to the bi-component chitosan/bioceramic materials significantly enhances adhesion, growth, and proliferation of osteoblast cells.
25064557	4	5	theme	cells	798:802	arg1	growth					758:763	growth	758:763	growth	758:763	Obtained data clearly prove that addition of 1,3-β-D-glucan to the bi-component chitosan/bioceramic materials significantly enhances adhesion, growth, and proliferation of osteoblast cells.
25064557	4	6	theme	bi-component	682:693	arg1	materials					715:723	the bi-component chitosan/bioceramic materials	678:723	the bi-component chitosan/bioceramic materials	678:723	Obtained data clearly prove that addition of 1,3-β-D-glucan to the bi-component chitosan/bioceramic materials significantly enhances adhesion, growth, and proliferation of osteoblast cells.
25064557	2	7	theme	direct-contact	414:427	arg1	method					429:434	direct-contact method	414:434	direct-contact method using live/dead double fluorescent staining	414:478	Cytotoxicity of materials was evaluated indirectly using fluid extracts and by direct-contact method using live/dead double fluorescent staining.
25064557	7	8	theme	bone	1108:1111	arg1	engineering					1120:1130	bone tissue engineering	1108:1130	bone tissue engineering application	1108:1142	Thus, it may be inferred that scaffolds modified with glucan are more promising materials for bone tissue engineering application than bi-component chitosan/bioceramic composites.
25064557	1	9	theme	osteoblastic	157:168	arg1	lines					175:179	two osteoblastic cell lines	153:179	two osteoblastic cell lines that addition of bacterial 1,3-β-D-glucan to chitosan-based biocomposites significantly enhances adhesion, growth, and proliferation of osteoblast cells	153:332	The aim of this work was to prove using two osteoblastic cell lines that addition of bacterial 1,3-β-D-glucan to chitosan-based biocomposites significantly enhances adhesion, growth, and proliferation of osteoblast cells.
25064557	4	10	theme	Obtained	615:622	arg1	data					624:627	Obtained data	615:627	Obtained data	615:627	Obtained data clearly prove that addition of 1,3-β-D-glucan to the bi-component chitosan/bioceramic materials significantly enhances adhesion, growth, and proliferation of osteoblast cells.
25064557	1	11	theme	cell	170:173	arg1	lines					175:179	two osteoblastic cell lines	153:179	two osteoblastic cell lines that addition of bacterial 1,3-β-D-glucan to chitosan-based biocomposites significantly enhances adhesion, growth, and proliferation of osteoblast cells	153:332	The aim of this work was to prove using two osteoblastic cell lines that addition of bacterial 1,3-β-D-glucan to chitosan-based biocomposites significantly enhances adhesion, growth, and proliferation of osteoblast cells.
25064557	2	12	theme	double	452:457	arg1	staining					471:478	live/dead double fluorescent staining	442:478	live/dead double fluorescent staining	442:478	Cytotoxicity of materials was evaluated indirectly using fluid extracts and by direct-contact method using live/dead double fluorescent staining.
25064557	2	13	theme	fluorescent	459:469	arg1	staining					471:478	live/dead double fluorescent staining	442:478	live/dead double fluorescent staining	442:478	Cytotoxicity of materials was evaluated indirectly using fluid extracts and by direct-contact method using live/dead double fluorescent staining.
25064557	6	14	located	observed	965:972	arg2	growth					954:959	significantly better osteoblast growth	922:959	significantly better osteoblast growth	922:959	However, significantly better osteoblast growth was observed on scaffolds containing 1,3-β-D-glucan.
25064557	6	14	located	observed	965:972	arg1	scaffolds					977:985	scaffolds	977:985	scaffolds containing 1,3-β-D-glucan	977:1011	However, significantly better osteoblast growth was observed on scaffolds containing 1,3-β-D-glucan.
25064557	2	15	theme	live/dead	442:450	arg1	staining					471:478	live/dead double fluorescent staining	442:478	live/dead double fluorescent staining	442:478	Cytotoxicity of materials was evaluated indirectly using fluid extracts and by direct-contact method using live/dead double fluorescent staining.
25064557	4	16	theme	osteoblast	787:796	arg1	cells					798:802	osteoblast cells	787:802	osteoblast cells	787:802	Obtained data clearly prove that addition of 1,3-β-D-glucan to the bi-component chitosan/bioceramic materials significantly enhances adhesion, growth, and proliferation of osteoblast cells.
25064557	0	17	theme	chitosan-based	30:43	arg1	composites					45:54	chitosan-based composites	30:54	chitosan-based composites	30:54	Addition of 1,3-β-D-glucan to chitosan-based composites enhances osteoblast adhesion, growth, and proliferation.
25064557	1	18	theme	bacterial	198:206	arg1	1,3-β-D-glucan					208:221	bacterial 1,3-β-D-glucan	198:221	bacterial 1,3-β-D-glucan	198:221	The aim of this work was to prove using two osteoblastic cell lines that addition of bacterial 1,3-β-D-glucan to chitosan-based biocomposites significantly enhances adhesion, growth, and proliferation of osteoblast cells.
25064557	0	19	theme	osteoblast	65:74	arg1	adhesion					76:83	osteoblast adhesion	65:83	osteoblast adhesion	65:83	Addition of 1,3-β-D-glucan to chitosan-based composites enhances osteoblast adhesion, growth, and proliferation.
25064557	1	20	theme	osteoblast	317:326	arg1	cells					328:332	osteoblast cells	317:332	osteoblast cells	317:332	The aim of this work was to prove using two osteoblastic cell lines that addition of bacterial 1,3-β-D-glucan to chitosan-based biocomposites significantly enhances adhesion, growth, and proliferation of osteoblast cells.
25064557	3	21	theme	total	532:536	arg1	test					538:541	total test	532:541	total test	532:541	Cell adhesion was determined quantitatively by LDH total test and cell proliferation was assessed by confocal microscope observation.
25064557	5	22	theme	cell	896:899	arg1	attachment					901:910	cell attachment	896:910	cell attachment	896:910	The results demonstrated that all investigated biomaterials were non-toxic and allowed for cell attachment.
25064557	1	23	theme	cells	328:332	arg1	growth					288:293	growth	288:293	growth	288:293	The aim of this work was to prove using two osteoblastic cell lines that addition of bacterial 1,3-β-D-glucan to chitosan-based biocomposites significantly enhances adhesion, growth, and proliferation of osteoblast cells.
25064557	1	23	theme	cells	328:332	arg1	proliferation					300:312	proliferation	300:312	proliferation	300:312	The aim of this work was to prove using two osteoblastic cell lines that addition of bacterial 1,3-β-D-glucan to chitosan-based biocomposites significantly enhances adhesion, growth, and proliferation of osteoblast cells.
25064557	1	23	theme	cells	328:332	arg1	adhesion					278:285	adhesion	278:285	adhesion	278:285	The aim of this work was to prove using two osteoblastic cell lines that addition of bacterial 1,3-β-D-glucan to chitosan-based biocomposites significantly enhances adhesion, growth, and proliferation of osteoblast cells.
25064557	7	24	theme	tissue	1113:1118	arg1	engineering					1120:1130	bone tissue engineering	1108:1130	bone tissue engineering application	1108:1142	Thus, it may be inferred that scaffolds modified with glucan are more promising materials for bone tissue engineering application than bi-component chitosan/bioceramic composites.
25064557	2	25	theme	materials	351:359	arg1	Cytotoxicity					335:346	Cytotoxicity	335:346	Cytotoxicity of materials	335:359	Cytotoxicity of materials was evaluated indirectly using fluid extracts and by direct-contact method using live/dead double fluorescent staining.
25064557	7	26	theme	bi-component	1149:1160	arg1	composites					1182:1191	bi-component chitosan/bioceramic composites	1149:1191	bi-component chitosan/bioceramic composites	1149:1191	Thus, it may be inferred that scaffolds modified with glucan are more promising materials for bone tissue engineering application than bi-component chitosan/bioceramic composites.
25064557	6	27	theme	osteoblast	943:952	arg1	growth					954:959	significantly better osteoblast growth	922:959	significantly better osteoblast growth	922:959	However, significantly better osteoblast growth was observed on scaffolds containing 1,3-β-D-glucan.
25064557	7	28	theme	engineering	1120:1130	arg1	application					1132:1142	bone tissue engineering application	1108:1142	bone tissue engineering application	1108:1142	Thus, it may be inferred that scaffolds modified with glucan are more promising materials for bone tissue engineering application than bi-component chitosan/bioceramic composites.
25064557	1	29	theme	1,3-β-D-glucan	208:221	arg1	addition					186:193	addition	186:193	addition of bacterial 1,3-β-D-glucan to chitosan-based biocomposites	186:253	The aim of this work was to prove using two osteoblastic cell lines that addition of bacterial 1,3-β-D-glucan to chitosan-based biocomposites significantly enhances adhesion, growth, and proliferation of osteoblast cells.
25064557	7	30	theme	chitosan/bioceramic	1162:1180	arg1	composites					1182:1191	bi-component chitosan/bioceramic composites	1149:1191	bi-component chitosan/bioceramic composites	1149:1191	Thus, it may be inferred that scaffolds modified with glucan are more promising materials for bone tissue engineering application than bi-component chitosan/bioceramic composites.
25064557	6	31	theme	better	936:941	arg1	growth					954:959	significantly better osteoblast growth	922:959	significantly better osteoblast growth	922:959	However, significantly better osteoblast growth was observed on scaffolds containing 1,3-β-D-glucan.
25064557	1	32	theme	work	129:132	arg1	aim					117:119	The aim	113:119	The aim of this work	113:132	The aim of this work was to prove using two osteoblastic cell lines that addition of bacterial 1,3-β-D-glucan to chitosan-based biocomposites significantly enhances adhesion, growth, and proliferation of osteoblast cells.
25064557	5	33	theme	investigated	839:850	arg1	biomaterials					852:863	all investigated biomaterials	835:863	all investigated biomaterials	835:863	The results demonstrated that all investigated biomaterials were non-toxic and allowed for cell attachment.
25064557	4	34	theme	chitosan/bioceramic	695:713	arg1	materials					715:723	the bi-component chitosan/bioceramic materials	678:723	the bi-component chitosan/bioceramic materials	678:723	Obtained data clearly prove that addition of 1,3-β-D-glucan to the bi-component chitosan/bioceramic materials significantly enhances adhesion, growth, and proliferation of osteoblast cells.
25064557	2	35	theme	fluid	392:396	arg1	extracts					398:405	fluid extracts	392:405	fluid extracts	392:405	Cytotoxicity of materials was evaluated indirectly using fluid extracts and by direct-contact method using live/dead double fluorescent staining.
25064557	3	36	theme	Cell	481:484	arg1	adhesion					486:493	Cell adhesion	481:493	Cell adhesion	481:493	Cell adhesion was determined quantitatively by LDH total test and cell proliferation was assessed by confocal microscope observation.
25064557	1	37	theme	chitosan-based	226:239	arg1	biocomposites					241:253	chitosan-based biocomposites	226:253	chitosan-based biocomposites	226:253	The aim of this work was to prove using two osteoblastic cell lines that addition of bacterial 1,3-β-D-glucan to chitosan-based biocomposites significantly enhances adhesion, growth, and proliferation of osteoblast cells.
25064557	0	38	theme	1,3-β-D-glucan	12:25	arg1	Addition					0:7	Addition	0:7	Addition of 1,3-β-D-glucan to chitosan-based composites	0:54	Addition of 1,3-β-D-glucan to chitosan-based composites enhances osteoblast adhesion, growth, and proliferation.
25064557	7	39	theme	promising	1084:1092	arg1	scaffolds					1044:1052	scaffolds	1044:1052	scaffolds modified with glucan	1044:1073	Thus, it may be inferred that scaffolds modified with glucan are more promising materials for bone tissue engineering application than bi-component chitosan/bioceramic composites.
25064557	7	39	theme	promising	1084:1092	arg1	materials					1094:1102	more promising materials	1079:1102	more promising materials for bone tissue engineering application	1079:1142	Thus, it may be inferred that scaffolds modified with glucan are more promising materials for bone tissue engineering application than bi-component chitosan/bioceramic composites.
25064557	4	40	theme	1,3-β-D-glucan	660:673	arg1	addition					648:655	addition	648:655	addition of 1,3-β-D-glucan to the bi-component chitosan/bioceramic materials	648:723	Obtained data clearly prove that addition of 1,3-β-D-glucan to the bi-component chitosan/bioceramic materials significantly enhances adhesion, growth, and proliferation of osteoblast cells.
25256552	5	0	theme	magnetic	712:719	arg1	beads					730:734	The magnetic alginate beads	708:734	The magnetic alginate beads	708:734	The magnetic alginate beads possess an enhanced SC of 14.29 mg/g than CaAlg composite and Fe3O4 particles which possess SC of 9.45 and 9.72 mg/g respectively.
25256552	7	1	theme	Langmuir	1074:1081	arg1	isotherms					1083:1091	Langmuir isotherms	1074:1091	Langmuir isotherms	1074:1091	The adsorption process was explained using Freundlich and Langmuir isotherms.
25256552	1	2	theme	scientists	146:155	arg1	attention					133:141	the attention	129:141	the attention of scientists	129:155	Recently magnetic bio-composites have attracted the attention of scientists because of their unique characteristics like selectivity and high sorption capacity.
25256552	6	3	theme	challenger	934:943	arg1	anions					945:950	challenger anions	934:950	challenger anions	934:950	The various sorption influencing parameters like contact time, pH, challenger anions, initial chromium concentration and temperature were optimized.
25256552	5	4	theme	mg/g	848:851	arg1	SC					828:829	SC	828:829	SC of 9.45 and 9.72 mg/g	828:851	The magnetic alginate beads possess an enhanced SC of 14.29 mg/g than CaAlg composite and Fe3O4 particles which possess SC of 9.45 and 9.72 mg/g respectively.
25256552	9	5	theme	chromium	1260:1267	arg1	sorption					1269:1276	chromium sorption	1260:1276	chromium sorption	1260:1276	The calculated thermodynamic parameters indicate the nature of chromium sorption is spontaneous and endothermic.
25256552	5	6	contain	possess	820:826	arg1	composite					784:792	CaAlg composite and Fe3O4 particles	778:812	composite	784:792	The magnetic alginate beads possess an enhanced SC of 14.29 mg/g than CaAlg composite and Fe3O4 particles which possess SC of 9.45 and 9.72 mg/g respectively.
25256552	5	6	contain	possess	820:826	arg1	particles					804:812	CaAlg composite and Fe3O4 particles	778:812	particles	804:812	The magnetic alginate beads possess an enhanced SC of 14.29 mg/g than CaAlg composite and Fe3O4 particles which possess SC of 9.45 and 9.72 mg/g respectively.
25256552	5	6	contain	possess	820:826	arg2	SC					828:829	SC	828:829	SC of 9.45 and 9.72 mg/g	828:851	The magnetic alginate beads possess an enhanced SC of 14.29 mg/g than CaAlg composite and Fe3O4 particles which possess SC of 9.45 and 9.72 mg/g respectively.
25256552	4	7	theme	composite	649:657	arg1	performance					592:602	A comparative adsorption performance	567:602	A comparative adsorption performance of Fe3O4 particles, calcium alginate (CaAlg) composite and Fe3O4@Alg-Ce magnetic hybrid beads	567:696	A comparative adsorption performance of Fe3O4 particles, calcium alginate (CaAlg) composite and Fe3O4@Alg-Ce magnetic hybrid beads was made.
25256552	6	8	theme	chromium	961:968	arg1	concentration					970:982	initial chromium concentration	953:982	initial chromium concentration	953:982	The various sorption influencing parameters like contact time, pH, challenger anions, initial chromium concentration and temperature were optimized.
25256552	9	9	theme	sorption	1269:1276	arg1	spontaneous					1281:1291	spontaneous	1281:1291	spontaneous	1281:1291	The calculated thermodynamic parameters indicate the nature of chromium sorption is spontaneous and endothermic.
25256552	9	9	theme	sorption	1269:1276	arg1	nature					1250:1255	the nature	1246:1255	the nature of chromium sorption	1246:1276	The calculated thermodynamic parameters indicate the nature of chromium sorption is spontaneous and endothermic.
25256552	2	10	theme	magnetic	277:284	arg1	beads					296:300	Fe3O4@Alg-Ce magnetic composite beads	264:300	Fe3O4@Alg-Ce magnetic composite beads	264:300	In the present study, Fe3O4@Alg-Ce magnetic composite beads were developed by incorporating Fe3O4 particles onto alginate (Alg) biopolymer followed by cross-linking with Ce(3+) ions.
25256552	1	11	theme	magnetic	90:97	arg1	bio-composites					99:112	magnetic bio-composites	90:112	magnetic bio-composites	90:112	Recently magnetic bio-composites have attracted the attention of scientists because of their unique characteristics like selectivity and high sorption capacity.
25256552	2	12	theme	Alg-Ce	270:275	arg1	beads					296:300	Fe3O4@Alg-Ce magnetic composite beads	264:300	Fe3O4@Alg-Ce magnetic composite beads	264:300	In the present study, Fe3O4@Alg-Ce magnetic composite beads were developed by incorporating Fe3O4 particles onto alginate (Alg) biopolymer followed by cross-linking with Ce(3+) ions.
25256552	2	13	theme	alginate	355:362	arg1	biopolymer					370:379	alginate (Alg) biopolymer	355:379	alginate (Alg) biopolymer followed by cross-linking with Ce(3+) ions	355:422	In the present study, Fe3O4@Alg-Ce magnetic composite beads were developed by incorporating Fe3O4 particles onto alginate (Alg) biopolymer followed by cross-linking with Ce(3+) ions.
25256552	4	14	theme	Alg-Ce	669:674	arg1	beads					692:696	Fe3O4@Alg-Ce magnetic hybrid beads	663:696	Fe3O4@Alg-Ce magnetic hybrid beads	663:696	A comparative adsorption performance of Fe3O4 particles, calcium alginate (CaAlg) composite and Fe3O4@Alg-Ce magnetic hybrid beads was made.
25256552	5	15	theme	alginate	721:728	arg1	beads					730:734	The magnetic alginate beads	708:734	The magnetic alginate beads	708:734	The magnetic alginate beads possess an enhanced SC of 14.29 mg/g than CaAlg composite and Fe3O4 particles which possess SC of 9.45 and 9.72 mg/g respectively.
25256552	2	16	with	cross-linking	393:405	arg1	ions					419:422	Ce(3+) ions	412:422	Ce(3+) ions	412:422	In the present study, Fe3O4@Alg-Ce magnetic composite beads were developed by incorporating Fe3O4 particles onto alginate (Alg) biopolymer followed by cross-linking with Ce(3+) ions.
25256552	5	17	theme	CaAlg	778:782	arg1	composite					784:792	CaAlg composite and Fe3O4 particles	778:812	composite	784:792	The magnetic alginate beads possess an enhanced SC of 14.29 mg/g than CaAlg composite and Fe3O4 particles which possess SC of 9.45 and 9.72 mg/g respectively.
25256552	2	18	theme	@	269:269	arg1	beads					296:300	Fe3O4@Alg-Ce magnetic composite beads	264:300	Fe3O4@Alg-Ce magnetic composite beads	264:300	In the present study, Fe3O4@Alg-Ce magnetic composite beads were developed by incorporating Fe3O4 particles onto alginate (Alg) biopolymer followed by cross-linking with Ce(3+) ions.
25256552	3	19	dep	removal	544:550	arg1	VI					540:541	VI	540:541	VI	540:541	The synthesized magnetic beads were characterized using FTIR and SEM with EDAX analysis and utilized for chromium (VI) removal in batch mode.
25256552	8	20	theme	particle	1171:1178	arg1	model					1190:1194	the pseudo second order and intra particle diffusion model	1137:1194	model	1190:1194	The sorption kinetics was fitted well with the pseudo second order and intra particle diffusion model.
25256552	8	21	theme	intra	1165:1169	arg1	model					1190:1194	the pseudo second order and intra particle diffusion model	1137:1194	model	1190:1194	The sorption kinetics was fitted well with the pseudo second order and intra particle diffusion model.
25256552	0	22	dep	removal	72:78	arg1	VI					68:69	VI	68:69	VI	68:69	Synthesis of magnetic alginate hybrid beads for efficient chromium (VI) removal.
25256552	5	23	theme	SC	756:757	arg1	mg/g					768:771	an enhanced SC of 14.29 mg/g	744:771	an enhanced SC of 14.29 mg/g than CaAlg composite and Fe3O4 particles which possess SC of 9.45 and 9.72 mg/g respectively	744:864	The magnetic alginate beads possess an enhanced SC of 14.29 mg/g than CaAlg composite and Fe3O4 particles which possess SC of 9.45 and 9.72 mg/g respectively.
25256552	4	24	theme	Fe3O4	607:611	arg1	particles					613:621	Fe3O4 particles	607:621	Fe3O4 particles	607:621	A comparative adsorption performance of Fe3O4 particles, calcium alginate (CaAlg) composite and Fe3O4@Alg-Ce magnetic hybrid beads was made.
25256552	4	25	theme	beads	692:696	arg1	performance					592:602	A comparative adsorption performance	567:602	A comparative adsorption performance of Fe3O4 particles, calcium alginate (CaAlg) composite and Fe3O4@Alg-Ce magnetic hybrid beads	567:696	A comparative adsorption performance of Fe3O4 particles, calcium alginate (CaAlg) composite and Fe3O4@Alg-Ce magnetic hybrid beads was made.
25256552	1	26	theme	unique	174:179	arg1	characteristics					181:195	their unique characteristics	168:195	their unique characteristics like selectivity and high sorption capacity	168:239	Recently magnetic bio-composites have attracted the attention of scientists because of their unique characteristics like selectivity and high sorption capacity.
25256552	5	27	theme	of	759:760	arg1	mg/g					768:771	an enhanced SC of 14.29 mg/g	744:771	an enhanced SC of 14.29 mg/g than CaAlg composite and Fe3O4 particles which possess SC of 9.45 and 9.72 mg/g respectively	744:864	The magnetic alginate beads possess an enhanced SC of 14.29 mg/g than CaAlg composite and Fe3O4 particles which possess SC of 9.45 and 9.72 mg/g respectively.
25256552	4	28	theme	calcium	624:630	arg1	composite					649:657	calcium alginate (CaAlg) composite	624:657	calcium alginate (CaAlg) composite	624:657	A comparative adsorption performance of Fe3O4 particles, calcium alginate (CaAlg) composite and Fe3O4@Alg-Ce magnetic hybrid beads was made.
25256552	4	29	theme	hybrid	685:690	arg1	beads					692:696	Fe3O4@Alg-Ce magnetic hybrid beads	663:696	Fe3O4@Alg-Ce magnetic hybrid beads	663:696	A comparative adsorption performance of Fe3O4 particles, calcium alginate (CaAlg) composite and Fe3O4@Alg-Ce magnetic hybrid beads was made.
25256552	3	30	used	utilized	517:524	arg2	beads					450:454	The synthesized magnetic beads	425:454	The synthesized magnetic beads	425:454	The synthesized magnetic beads were characterized using FTIR and SEM with EDAX analysis and utilized for chromium (VI) removal in batch mode.
25256552	0	31	theme	alginate	22:29	arg1	beads					38:42	magnetic alginate hybrid beads	13:42	magnetic alginate hybrid beads	13:42	Synthesis of magnetic alginate hybrid beads for efficient chromium (VI) removal.
25256552	6	32	theme	contact	916:922	arg1	time					924:927	contact time	916:927	contact time	916:927	The various sorption influencing parameters like contact time, pH, challenger anions, initial chromium concentration and temperature were optimized.
25256552	3	33	theme	chromium	530:537	arg1	removal					544:550	chromium (VI) removal	530:550	chromium (VI) removal	530:550	The synthesized magnetic beads were characterized using FTIR and SEM with EDAX analysis and utilized for chromium (VI) removal in batch mode.
25256552	3	34	theme	magnetic	441:448	arg1	beads					450:454	The synthesized magnetic beads	425:454	The synthesized magnetic beads	425:454	The synthesized magnetic beads were characterized using FTIR and SEM with EDAX analysis and utilized for chromium (VI) removal in batch mode.
25256552	0	35	theme	magnetic	13:20	arg1	beads					38:42	magnetic alginate hybrid beads	13:42	magnetic alginate hybrid beads	13:42	Synthesis of magnetic alginate hybrid beads for efficient chromium (VI) removal.
25256552	7	36	theme	adsorption	1020:1029	arg1	process					1031:1037	The adsorption process	1016:1037	The adsorption process	1016:1037	The adsorption process was explained using Freundlich and Langmuir isotherms.
25256552	2	37	theme	composite	286:294	arg1	beads					296:300	Fe3O4@Alg-Ce magnetic composite beads	264:300	Fe3O4@Alg-Ce magnetic composite beads	264:300	In the present study, Fe3O4@Alg-Ce magnetic composite beads were developed by incorporating Fe3O4 particles onto alginate (Alg) biopolymer followed by cross-linking with Ce(3+) ions.
25256552	2	38	theme	Ce	412:413	arg1	ions					419:422	Ce(3+) ions	412:422	Ce(3+) ions	412:422	In the present study, Fe3O4@Alg-Ce magnetic composite beads were developed by incorporating Fe3O4 particles onto alginate (Alg) biopolymer followed by cross-linking with Ce(3+) ions.
25256552	0	39	theme	beads	38:42	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of magnetic alginate hybrid beads for efficient chromium (VI) removal	0:78	Synthesis of magnetic alginate hybrid beads for efficient chromium (VI) removal.
25256552	4	40	theme	Fe3O4	663:667	arg1	beads					692:696	Fe3O4@Alg-Ce magnetic hybrid beads	663:696	Fe3O4@Alg-Ce magnetic hybrid beads	663:696	A comparative adsorption performance of Fe3O4 particles, calcium alginate (CaAlg) composite and Fe3O4@Alg-Ce magnetic hybrid beads was made.
25256552	3	41	theme	batch	555:559	arg1	mode					561:564	batch mode	555:564	batch mode	555:564	The synthesized magnetic beads were characterized using FTIR and SEM with EDAX analysis and utilized for chromium (VI) removal in batch mode.
25256552	4	42	theme	alginate	632:639	arg1	composite					649:657	calcium alginate (CaAlg) composite	624:657	calcium alginate (CaAlg) composite	624:657	A comparative adsorption performance of Fe3O4 particles, calcium alginate (CaAlg) composite and Fe3O4@Alg-Ce magnetic hybrid beads was made.
25256552	4	43	theme	particles	613:621	arg1	performance					592:602	A comparative adsorption performance	567:602	A comparative adsorption performance of Fe3O4 particles, calcium alginate (CaAlg) composite and Fe3O4@Alg-Ce magnetic hybrid beads	567:696	A comparative adsorption performance of Fe3O4 particles, calcium alginate (CaAlg) composite and Fe3O4@Alg-Ce magnetic hybrid beads was made.
25256552	0	44	theme	hybrid	31:36	arg1	beads					38:42	magnetic alginate hybrid beads	13:42	magnetic alginate hybrid beads	13:42	Synthesis of magnetic alginate hybrid beads for efficient chromium (VI) removal.
25256552	6	45	theme	influencing	888:898	arg1	parameters					900:909	The various sorption influencing parameters	867:909	The various sorption influencing parameters like contact time, pH, challenger anions, initial chromium concentration and temperature	867:998	The various sorption influencing parameters like contact time, pH, challenger anions, initial chromium concentration and temperature were optimized.
25256552	8	46	theme	sorption	1098:1105	arg1	kinetics					1107:1114	The sorption kinetics	1094:1114	The sorption kinetics	1094:1114	The sorption kinetics was fitted well with the pseudo second order and intra particle diffusion model.
25256552	1	47	theme	high	218:221	arg1	capacity					232:239	high sorption capacity	218:239	high sorption capacity	218:239	Recently magnetic bio-composites have attracted the attention of scientists because of their unique characteristics like selectivity and high sorption capacity.
25256552	0	48	theme	efficient	48:56	arg1	removal					72:78	efficient chromium (VI) removal	48:78	efficient chromium (VI) removal	48:78	Synthesis of magnetic alginate hybrid beads for efficient chromium (VI) removal.
25256552	6	49	theme	sorption	879:886	arg1	parameters					900:909	The various sorption influencing parameters	867:909	The various sorption influencing parameters like contact time, pH, challenger anions, initial chromium concentration and temperature	867:998	The various sorption influencing parameters like contact time, pH, challenger anions, initial chromium concentration and temperature were optimized.
25256552	9	50	theme	calculated	1201:1210	arg1	parameters					1226:1235	The calculated thermodynamic parameters	1197:1235	The calculated thermodynamic parameters	1197:1235	The calculated thermodynamic parameters indicate the nature of chromium sorption is spontaneous and endothermic.
25256552	4	51	theme	@	668:668	arg1	beads					692:696	Fe3O4@Alg-Ce magnetic hybrid beads	663:696	Fe3O4@Alg-Ce magnetic hybrid beads	663:696	A comparative adsorption performance of Fe3O4 particles, calcium alginate (CaAlg) composite and Fe3O4@Alg-Ce magnetic hybrid beads was made.
25256552	5	52	theme	enhanced	747:754	arg1	mg/g					768:771	an enhanced SC of 14.29 mg/g	744:771	an enhanced SC of 14.29 mg/g than CaAlg composite and Fe3O4 particles which possess SC of 9.45 and 9.72 mg/g respectively	744:864	The magnetic alginate beads possess an enhanced SC of 14.29 mg/g than CaAlg composite and Fe3O4 particles which possess SC of 9.45 and 9.72 mg/g respectively.
25256552	6	53	theme	various	871:877	arg1	parameters					900:909	The various sorption influencing parameters	867:909	The various sorption influencing parameters like contact time, pH, challenger anions, initial chromium concentration and temperature	867:998	The various sorption influencing parameters like contact time, pH, challenger anions, initial chromium concentration and temperature were optimized.
25256552	9	54	theme	thermodynamic	1212:1224	arg1	parameters					1226:1235	The calculated thermodynamic parameters	1197:1235	The calculated thermodynamic parameters	1197:1235	The calculated thermodynamic parameters indicate the nature of chromium sorption is spontaneous and endothermic.
25256552	2	55	theme	Fe3O4	334:338	arg1	particles					340:348	Fe3O4 particles	334:348	Fe3O4 particles	334:348	In the present study, Fe3O4@Alg-Ce magnetic composite beads were developed by incorporating Fe3O4 particles onto alginate (Alg) biopolymer followed by cross-linking with Ce(3+) ions.
25256552	8	56	theme	pseudo	1141:1146	arg1	order					1155:1159	the pseudo second order and intra particle diffusion model	1137:1194	order	1155:1159	The sorption kinetics was fitted well with the pseudo second order and intra particle diffusion model.
25256552	4	57	theme	magnetic	676:683	arg1	beads					692:696	Fe3O4@Alg-Ce magnetic hybrid beads	663:696	Fe3O4@Alg-Ce magnetic hybrid beads	663:696	A comparative adsorption performance of Fe3O4 particles, calcium alginate (CaAlg) composite and Fe3O4@Alg-Ce magnetic hybrid beads was made.
25256552	1	58	theme	sorption	223:230	arg1	capacity					232:239	high sorption capacity	218:239	high sorption capacity	218:239	Recently magnetic bio-composites have attracted the attention of scientists because of their unique characteristics like selectivity and high sorption capacity.
25256552	2	59	theme	Fe3O4	264:268	arg1	beads					296:300	Fe3O4@Alg-Ce magnetic composite beads	264:300	Fe3O4@Alg-Ce magnetic composite beads	264:300	In the present study, Fe3O4@Alg-Ce magnetic composite beads were developed by incorporating Fe3O4 particles onto alginate (Alg) biopolymer followed by cross-linking with Ce(3+) ions.
25256552	5	60	contain	possess	736:742	arg2	mg/g					768:771	an enhanced SC of 14.29 mg/g	744:771	an enhanced SC of 14.29 mg/g than CaAlg composite and Fe3O4 particles which possess SC of 9.45 and 9.72 mg/g respectively	744:864	The magnetic alginate beads possess an enhanced SC of 14.29 mg/g than CaAlg composite and Fe3O4 particles which possess SC of 9.45 and 9.72 mg/g respectively.
25256552	5	60	contain	possess	736:742	arg1	beads					730:734	The magnetic alginate beads	708:734	The magnetic alginate beads	708:734	The magnetic alginate beads possess an enhanced SC of 14.29 mg/g than CaAlg composite and Fe3O4 particles which possess SC of 9.45 and 9.72 mg/g respectively.
25256552	5	61	theme	Fe3O4	798:802	arg1	particles					804:812	CaAlg composite and Fe3O4 particles	778:812	particles	804:812	The magnetic alginate beads possess an enhanced SC of 14.29 mg/g than CaAlg composite and Fe3O4 particles which possess SC of 9.45 and 9.72 mg/g respectively.
25256552	4	62	theme	CaAlg	642:646	arg1	composite					649:657	calcium alginate (CaAlg) composite	624:657	calcium alginate (CaAlg) composite	624:657	A comparative adsorption performance of Fe3O4 particles, calcium alginate (CaAlg) composite and Fe3O4@Alg-Ce magnetic hybrid beads was made.
25256552	8	63	theme	diffusion	1180:1188	arg1	model					1190:1194	the pseudo second order and intra particle diffusion model	1137:1194	model	1190:1194	The sorption kinetics was fitted well with the pseudo second order and intra particle diffusion model.
25256552	6	64	theme	initial	953:959	arg1	concentration					970:982	initial chromium concentration	953:982	initial chromium concentration	953:982	The various sorption influencing parameters like contact time, pH, challenger anions, initial chromium concentration and temperature were optimized.
25256552	3	65	theme	synthesized	429:439	arg1	beads					450:454	The synthesized magnetic beads	425:454	The synthesized magnetic beads	425:454	The synthesized magnetic beads were characterized using FTIR and SEM with EDAX analysis and utilized for chromium (VI) removal in batch mode.
25256552	2	66	theme	present	249:255	arg1	study					257:261	the present study	245:261	the present study	245:261	In the present study, Fe3O4@Alg-Ce magnetic composite beads were developed by incorporating Fe3O4 particles onto alginate (Alg) biopolymer followed by cross-linking with Ce(3+) ions.
25256552	2	67	theme	Alg	365:367	arg1	biopolymer					370:379	alginate (Alg) biopolymer	355:379	alginate (Alg) biopolymer followed by cross-linking with Ce(3+) ions	355:422	In the present study, Fe3O4@Alg-Ce magnetic composite beads were developed by incorporating Fe3O4 particles onto alginate (Alg) biopolymer followed by cross-linking with Ce(3+) ions.
25256552	4	68	theme	adsorption	581:590	arg1	performance					592:602	A comparative adsorption performance	567:602	A comparative adsorption performance of Fe3O4 particles, calcium alginate (CaAlg) composite and Fe3O4@Alg-Ce magnetic hybrid beads	567:696	A comparative adsorption performance of Fe3O4 particles, calcium alginate (CaAlg) composite and Fe3O4@Alg-Ce magnetic hybrid beads was made.
25256552	0	69	theme	chromium	58:65	arg1	removal					72:78	efficient chromium (VI) removal	48:78	efficient chromium (VI) removal	48:78	Synthesis of magnetic alginate hybrid beads for efficient chromium (VI) removal.
25256552	8	70	theme	second	1148:1153	arg1	order					1155:1159	the pseudo second order and intra particle diffusion model	1137:1194	order	1155:1159	The sorption kinetics was fitted well with the pseudo second order and intra particle diffusion model.
25256552	3	71	theme	EDAX	499:502	arg1	analysis					504:511	EDAX analysis	499:511	EDAX analysis	499:511	The synthesized magnetic beads were characterized using FTIR and SEM with EDAX analysis and utilized for chromium (VI) removal in batch mode.
25256552	4	72	theme	comparative	569:579	arg1	performance					592:602	A comparative adsorption performance	567:602	A comparative adsorption performance of Fe3O4 particles, calcium alginate (CaAlg) composite and Fe3O4@Alg-Ce magnetic hybrid beads	567:696	A comparative adsorption performance of Fe3O4 particles, calcium alginate (CaAlg) composite and Fe3O4@Alg-Ce magnetic hybrid beads was made.
28807689	3	0	theme	d-glucuronic	639:650	arg1	acid					652:655	d-glucuronic acid	639:655	d-glucuronic acid	639:655	The main monosaccharide compositions of AVP were d-galactose, d-xylose, d-mannose, d-glucose and d-glucuronic acid.
28807689	2	1	theme	radical	423:429	arg1	contents					455:462	uronic acid, sulfate radical, hexosamine and protein contents	402:462	uronic acid, sulfate radical, hexosamine and protein contents	402:462	The total sugar content was 46.27±1.5% and uronic acid, sulfate radical, hexosamine and protein contents were 17.44±0.22%, 16.98±0.15%, 0.65±0.02% and 1.64±0.13% in AVP respectively.
28807689	0	2	dep	proliferation	94:106	arg1	Effects					0:6	Effects	0:6	Effects of polysaccharides from abalone viscera (Haliotis discus hannai Ino) on MGC 803 cells	0:92	Effects of polysaccharides from abalone viscera (Haliotis discus hannai Ino) on MGC 803 cells proliferation.
28807689	2	3	from	%	396:396	arg1	AVP					524:526	AVP	524:526	AVP	524:526	The total sugar content was 46.27±1.5% and uronic acid, sulfate radical, hexosamine and protein contents were 17.44±0.22%, 16.98±0.15%, 0.65±0.02% and 1.64±0.13% in AVP respectively.
28807689	1	4	theme	alkaline	209:216	arg1	method					250:255	sevage method	243:255	sevage method	243:255	The polysaccharides (AVP) was obtained from abalone (Haliotis discus hannai Ino) viscera, using the alkaline protease to enzymolysis, sevage method and repeated freezing and thawing method to remove protein and hydrogen peroxide method to depigment.
28807689	1	4	theme	alkaline	209:216	arg1	protease					218:225	the alkaline protease	205:225	the alkaline protease to enzymolysis	205:240	The polysaccharides (AVP) was obtained from abalone (Haliotis discus hannai Ino) viscera, using the alkaline protease to enzymolysis, sevage method and repeated freezing and thawing method to remove protein and hydrogen peroxide method to depigment.
28807689	1	4	theme	alkaline	209:216	arg1	method					291:296	repeated freezing and thawing method	261:296	repeated freezing and thawing method	261:296	The polysaccharides (AVP) was obtained from abalone (Haliotis discus hannai Ino) viscera, using the alkaline protease to enzymolysis, sevage method and repeated freezing and thawing method to remove protein and hydrogen peroxide method to depigment.
28807689	0	5	theme	MGC	80:82	arg1	cells					88:92	MGC 803 cells	80:92	MGC 803 cells	80:92	Effects of polysaccharides from abalone viscera (Haliotis discus hannai Ino) on MGC 803 cells proliferation.
28807689	0	6	from	Effects	0:6	arg1	Ino					72:74	Haliotis discus hannai Ino	49:74	Haliotis discus hannai Ino	49:74	Effects of polysaccharides from abalone viscera (Haliotis discus hannai Ino) on MGC 803 cells proliferation.
28807689	0	6	from	Effects	0:6	arg1	viscera					40:46	abalone viscera	32:46	abalone viscera (Haliotis discus hannai Ino)	32:75	Effects of polysaccharides from abalone viscera (Haliotis discus hannai Ino) on MGC 803 cells proliferation.
28807689	0	6	from	Effects	0:6	arg1	cells					88:92	MGC 803 cells	80:92	MGC 803 cells	80:92	Effects of polysaccharides from abalone viscera (Haliotis discus hannai Ino) on MGC 803 cells proliferation.
28807689	5	7	theme	Morphological	859:871	arg1	changes					873:879	Morphological changes	859:879	Morphological changes	859:879	The results of Morphological changes, cell migration ability and AO/EB staining indicated that MGC803 cells underwent apoptosis in a dose-dependent manner induced by AVP.
28807689	4	8	contain	had	777:779	arg2	influence					784:792	no influence	781:792	no influence	781:792	MTT assay showed AVP had a significant anti-tumor activity to gastric carcinoma cells, especially to MGC 803, while it had no influence upon proliferation of normal stomach cells GES 1.
28807689	4	8	contain	had	777:779	arg1	it					774:775	it	774:775	it	774:775	MTT assay showed AVP had a significant anti-tumor activity to gastric carcinoma cells, especially to MGC 803, while it had no influence upon proliferation of normal stomach cells GES 1.
28807689	6	9	dep	blotting	1037:1044	arg1	results					1046:1052	results	1046:1052	the western blotting results	1025:1052	Moreover, the western blotting results showed that the expressions of survivin, Bcl-2 and VEGF were decreased, while the expression of Bax and p53 were increased in a dose-dependent manner of AVP.
28807689	1	10	theme	protein	308:314	arg1	method					338:343	protein and hydrogen peroxide method	308:343	protein and hydrogen peroxide method to depigment	308:356	The polysaccharides (AVP) was obtained from abalone (Haliotis discus hannai Ino) viscera, using the alkaline protease to enzymolysis, sevage method and repeated freezing and thawing method to remove protein and hydrogen peroxide method to depigment.
28807689	6	11	theme	p53	1158:1160	arg1	expression					1136:1145	the expression	1132:1145	the expression of Bax and p53	1132:1160	Moreover, the western blotting results showed that the expressions of survivin, Bcl-2 and VEGF were decreased, while the expression of Bax and p53 were increased in a dose-dependent manner of AVP.
28807689	5	12	theme	changes	873:879	arg1	results					848:854	The results	844:854	The results of Morphological changes, cell migration ability and AO/EB staining	844:922	The results of Morphological changes, cell migration ability and AO/EB staining indicated that MGC803 cells underwent apoptosis in a dose-dependent manner induced by AVP.
28807689	2	13	from	%	504:504	arg1	AVP					524:526	AVP	524:526	AVP	524:526	The total sugar content was 46.27±1.5% and uronic acid, sulfate radical, hexosamine and protein contents were 17.44±0.22%, 16.98±0.15%, 0.65±0.02% and 1.64±0.13% in AVP respectively.
28807689	4	14	dep	cells	831:835	arg1	GES					837:839	GES 1	837:841	normal stomach cells GES 1	816:841	MTT assay showed AVP had a significant anti-tumor activity to gastric carcinoma cells, especially to MGC 803, while it had no influence upon proliferation of normal stomach cells GES 1.
28807689	2	15	theme	sugar	369:373	arg1	%					396:396	46.27±1.5%	387:396	46.27±1.5%	387:396	The total sugar content was 46.27±1.5% and uronic acid, sulfate radical, hexosamine and protein contents were 17.44±0.22%, 16.98±0.15%, 0.65±0.02% and 1.64±0.13% in AVP respectively.
28807689	2	15	theme	sugar	369:373	arg1	content					375:381	The total sugar content	359:381	The total sugar content	359:381	The total sugar content was 46.27±1.5% and uronic acid, sulfate radical, hexosamine and protein contents were 17.44±0.22%, 16.98±0.15%, 0.65±0.02% and 1.64±0.13% in AVP respectively.
28807689	4	16	theme	anti-tumor	697:706	arg1	activity					708:715	a significant anti-tumor activity	683:715	a significant anti-tumor activity	683:715	MTT assay showed AVP had a significant anti-tumor activity to gastric carcinoma cells, especially to MGC 803, while it had no influence upon proliferation of normal stomach cells GES 1.
28807689	6	17	theme	VEGF	1105:1108	arg1	expressions					1070:1080	the expressions	1066:1080	the expressions of survivin, Bcl-2 and VEGF	1066:1108	Moreover, the western blotting results showed that the expressions of survivin, Bcl-2 and VEGF were decreased, while the expression of Bax and p53 were increased in a dose-dependent manner of AVP.
28807689	2	18	theme	protein	447:453	arg1	contents					455:462	uronic acid, sulfate radical, hexosamine and protein contents	402:462	uronic acid, sulfate radical, hexosamine and protein contents	402:462	The total sugar content was 46.27±1.5% and uronic acid, sulfate radical, hexosamine and protein contents were 17.44±0.22%, 16.98±0.15%, 0.65±0.02% and 1.64±0.13% in AVP respectively.
28807689	0	19	from	viscera	40:46	arg1	polysaccharides					11:25	polysaccharides	11:25	polysaccharides from abalone viscera (Haliotis discus hannai Ino)	11:75	Effects of polysaccharides from abalone viscera (Haliotis discus hannai Ino) on MGC 803 cells proliferation.
28807689	0	19	from	viscera	40:46	arg1	Effects					0:6	Effects	0:6	Effects of polysaccharides from abalone viscera (Haliotis discus hannai Ino) on MGC 803 cells	0:92	Effects of polysaccharides from abalone viscera (Haliotis discus hannai Ino) on MGC 803 cells proliferation.
28807689	6	20	theme	Bax	1150:1152	arg1	expression					1136:1145	the expression	1132:1145	the expression of Bax and p53	1132:1160	Moreover, the western blotting results showed that the expressions of survivin, Bcl-2 and VEGF were decreased, while the expression of Bax and p53 were increased in a dose-dependent manner of AVP.
28807689	4	21	theme	significant	685:695	arg1	activity					708:715	a significant anti-tumor activity	683:715	a significant anti-tumor activity	683:715	MTT assay showed AVP had a significant anti-tumor activity to gastric carcinoma cells, especially to MGC 803, while it had no influence upon proliferation of normal stomach cells GES 1.
28807689	6	22	theme	survivin	1085:1092	arg1	expressions					1070:1080	the expressions	1066:1080	the expressions of survivin, Bcl-2 and VEGF	1066:1108	Moreover, the western blotting results showed that the expressions of survivin, Bcl-2 and VEGF were decreased, while the expression of Bax and p53 were increased in a dose-dependent manner of AVP.
28807689	5	23	theme	ability	897:903	arg1	results					848:854	The results	844:854	The results of Morphological changes, cell migration ability and AO/EB staining	844:922	The results of Morphological changes, cell migration ability and AO/EB staining indicated that MGC803 cells underwent apoptosis in a dose-dependent manner induced by AVP.
28807689	1	24	theme	peroxide	329:336	arg1	method					338:343	protein and hydrogen peroxide method	308:343	protein and hydrogen peroxide method to depigment	308:356	The polysaccharides (AVP) was obtained from abalone (Haliotis discus hannai Ino) viscera, using the alkaline protease to enzymolysis, sevage method and repeated freezing and thawing method to remove protein and hydrogen peroxide method to depigment.
28807689	1	25	theme	abalone	153:159	arg1	viscera					190:196	abalone (Haliotis discus hannai Ino) viscera	153:196	abalone (Haliotis discus hannai Ino) viscera	153:196	The polysaccharides (AVP) was obtained from abalone (Haliotis discus hannai Ino) viscera, using the alkaline protease to enzymolysis, sevage method and repeated freezing and thawing method to remove protein and hydrogen peroxide method to depigment.
28807689	2	26	from	%	519:519	arg1	AVP					524:526	AVP	524:526	AVP	524:526	The total sugar content was 46.27±1.5% and uronic acid, sulfate radical, hexosamine and protein contents were 17.44±0.22%, 16.98±0.15%, 0.65±0.02% and 1.64±0.13% in AVP respectively.
28807689	4	27	theme	MTT	658:660	arg1	assay					662:666	MTT assay	658:666	MTT assay	658:666	MTT assay showed AVP had a significant anti-tumor activity to gastric carcinoma cells, especially to MGC 803, while it had no influence upon proliferation of normal stomach cells GES 1.
28807689	3	28	theme	AVP	582:584	arg1	d-galactose					591:601	d-galactose	591:601	d-galactose	591:601	The main monosaccharide compositions of AVP were d-galactose, d-xylose, d-mannose, d-glucose and d-glucuronic acid.
28807689	3	28	theme	AVP	582:584	arg1	compositions					566:577	The main monosaccharide compositions	542:577	The main monosaccharide compositions of AVP	542:584	The main monosaccharide compositions of AVP were d-galactose, d-xylose, d-mannose, d-glucose and d-glucuronic acid.
28807689	6	29	theme	dose-dependent	1182:1195	arg1	manner					1197:1202	a dose-dependent manner	1180:1202	a dose-dependent manner of AVP	1180:1209	Moreover, the western blotting results showed that the expressions of survivin, Bcl-2 and VEGF were decreased, while the expression of Bax and p53 were increased in a dose-dependent manner of AVP.
28807689	4	30	theme	cells	831:835	arg1	proliferation					799:811	proliferation	799:811	proliferation of normal stomach cells GES 1	799:841	MTT assay showed AVP had a significant anti-tumor activity to gastric carcinoma cells, especially to MGC 803, while it had no influence upon proliferation of normal stomach cells GES 1.
28807689	7	31	theme	potential	1254:1262	arg1	AVP					1239:1241	AVP	1239:1241	AVP	1239:1241	The results suggested that AVP might be a potential anti-tumor agent securely and naturally.
28807689	7	31	theme	potential	1254:1262	arg1	agent					1275:1279	a potential anti-tumor agent	1252:1279	a potential anti-tumor agent	1252:1279	The results suggested that AVP might be a potential anti-tumor agent securely and naturally.
28807689	1	32	theme	Haliotis	162:169	arg1	abalone					153:159	abalone	153:159	abalone (Haliotis discus hannai Ino) viscera	153:196	The polysaccharides (AVP) was obtained from abalone (Haliotis discus hannai Ino) viscera, using the alkaline protease to enzymolysis, sevage method and repeated freezing and thawing method to remove protein and hydrogen peroxide method to depigment.
28807689	1	32	theme	Haliotis	162:169	arg1	Ino					185:187	Haliotis discus hannai Ino	162:187	Haliotis discus hannai Ino	162:187	The polysaccharides (AVP) was obtained from abalone (Haliotis discus hannai Ino) viscera, using the alkaline protease to enzymolysis, sevage method and repeated freezing and thawing method to remove protein and hydrogen peroxide method to depigment.
28807689	0	33	theme	polysaccharides	11:25	arg1	Effects					0:6	Effects	0:6	Effects of polysaccharides from abalone viscera (Haliotis discus hannai Ino) on MGC 803 cells	0:92	Effects of polysaccharides from abalone viscera (Haliotis discus hannai Ino) on MGC 803 cells proliferation.
28807689	1	34	theme	sevage	243:248	arg1	method					250:255	sevage method	243:255	sevage method	243:255	The polysaccharides (AVP) was obtained from abalone (Haliotis discus hannai Ino) viscera, using the alkaline protease to enzymolysis, sevage method and repeated freezing and thawing method to remove protein and hydrogen peroxide method to depigment.
28807689	1	34	theme	sevage	243:248	arg1	protease					218:225	the alkaline protease	205:225	the alkaline protease to enzymolysis	205:240	The polysaccharides (AVP) was obtained from abalone (Haliotis discus hannai Ino) viscera, using the alkaline protease to enzymolysis, sevage method and repeated freezing and thawing method to remove protein and hydrogen peroxide method to depigment.
28807689	4	35	theme	stomach	823:829	arg1	cells					831:835	normal stomach cells GES 1	816:841	normal stomach cells GES 1	816:841	MTT assay showed AVP had a significant anti-tumor activity to gastric carcinoma cells, especially to MGC 803, while it had no influence upon proliferation of normal stomach cells GES 1.
28807689	2	36	theme	acid	409:412	arg1	contents					455:462	uronic acid, sulfate radical, hexosamine and protein contents	402:462	uronic acid, sulfate radical, hexosamine and protein contents	402:462	The total sugar content was 46.27±1.5% and uronic acid, sulfate radical, hexosamine and protein contents were 17.44±0.22%, 16.98±0.15%, 0.65±0.02% and 1.64±0.13% in AVP respectively.
28807689	1	37	theme	discus	171:176	arg1	abalone					153:159	abalone	153:159	abalone (Haliotis discus hannai Ino) viscera	153:196	The polysaccharides (AVP) was obtained from abalone (Haliotis discus hannai Ino) viscera, using the alkaline protease to enzymolysis, sevage method and repeated freezing and thawing method to remove protein and hydrogen peroxide method to depigment.
28807689	1	37	theme	discus	171:176	arg1	Ino					185:187	Haliotis discus hannai Ino	162:187	Haliotis discus hannai Ino	162:187	The polysaccharides (AVP) was obtained from abalone (Haliotis discus hannai Ino) viscera, using the alkaline protease to enzymolysis, sevage method and repeated freezing and thawing method to remove protein and hydrogen peroxide method to depigment.
28807689	2	38	theme	sulfate	415:421	arg1	radical					423:429	sulfate radical	415:429	sulfate radical	415:429	The total sugar content was 46.27±1.5% and uronic acid, sulfate radical, hexosamine and protein contents were 17.44±0.22%, 16.98±0.15%, 0.65±0.02% and 1.64±0.13% in AVP respectively.
28807689	6	39	theme	western	1029:1035	arg1	blotting					1037:1044	the western blotting results	1025:1052	the western blotting results	1025:1052	Moreover, the western blotting results showed that the expressions of survivin, Bcl-2 and VEGF were decreased, while the expression of Bax and p53 were increased in a dose-dependent manner of AVP.
28807689	2	40	theme	total	363:367	arg1	%					396:396	46.27±1.5%	387:396	46.27±1.5%	387:396	The total sugar content was 46.27±1.5% and uronic acid, sulfate radical, hexosamine and protein contents were 17.44±0.22%, 16.98±0.15%, 0.65±0.02% and 1.64±0.13% in AVP respectively.
28807689	2	40	theme	total	363:367	arg1	content					375:381	The total sugar content	359:381	The total sugar content	359:381	The total sugar content was 46.27±1.5% and uronic acid, sulfate radical, hexosamine and protein contents were 17.44±0.22%, 16.98±0.15%, 0.65±0.02% and 1.64±0.13% in AVP respectively.
28807689	5	41	theme	staining	915:922	arg1	results					848:854	The results	844:854	The results of Morphological changes, cell migration ability and AO/EB staining	844:922	The results of Morphological changes, cell migration ability and AO/EB staining indicated that MGC803 cells underwent apoptosis in a dose-dependent manner induced by AVP.
28807689	6	42	theme	Bcl-2	1095:1099	arg1	expressions					1070:1080	the expressions	1066:1080	the expressions of survivin, Bcl-2 and VEGF	1066:1108	Moreover, the western blotting results showed that the expressions of survivin, Bcl-2 and VEGF were decreased, while the expression of Bax and p53 were increased in a dose-dependent manner of AVP.
28807689	1	43	theme	hannai	178:183	arg1	abalone					153:159	abalone	153:159	abalone (Haliotis discus hannai Ino) viscera	153:196	The polysaccharides (AVP) was obtained from abalone (Haliotis discus hannai Ino) viscera, using the alkaline protease to enzymolysis, sevage method and repeated freezing and thawing method to remove protein and hydrogen peroxide method to depigment.
28807689	1	43	theme	hannai	178:183	arg1	Ino					185:187	Haliotis discus hannai Ino	162:187	Haliotis discus hannai Ino	162:187	The polysaccharides (AVP) was obtained from abalone (Haliotis discus hannai Ino) viscera, using the alkaline protease to enzymolysis, sevage method and repeated freezing and thawing method to remove protein and hydrogen peroxide method to depigment.
28807689	0	44	theme	abalone	32:38	arg1	Ino					72:74	Haliotis discus hannai Ino	49:74	Haliotis discus hannai Ino	49:74	Effects of polysaccharides from abalone viscera (Haliotis discus hannai Ino) on MGC 803 cells proliferation.
28807689	0	44	theme	abalone	32:38	arg1	viscera					40:46	abalone viscera	32:46	abalone viscera (Haliotis discus hannai Ino)	32:75	Effects of polysaccharides from abalone viscera (Haliotis discus hannai Ino) on MGC 803 cells proliferation.
28807689	6	45	theme	AVP	1207:1209	arg1	manner					1197:1202	a dose-dependent manner	1180:1202	a dose-dependent manner of AVP	1180:1209	Moreover, the western blotting results showed that the expressions of survivin, Bcl-2 and VEGF were decreased, while the expression of Bax and p53 were increased in a dose-dependent manner of AVP.
28807689	3	46	theme	monosaccharide	551:564	arg1	d-galactose					591:601	d-galactose	591:601	d-galactose	591:601	The main monosaccharide compositions of AVP were d-galactose, d-xylose, d-mannose, d-glucose and d-glucuronic acid.
28807689	3	46	theme	monosaccharide	551:564	arg1	compositions					566:577	The main monosaccharide compositions	542:577	The main monosaccharide compositions of AVP	542:584	The main monosaccharide compositions of AVP were d-galactose, d-xylose, d-mannose, d-glucose and d-glucuronic acid.
28807689	7	47	theme	anti-tumor	1264:1273	arg1	AVP					1239:1241	AVP	1239:1241	AVP	1239:1241	The results suggested that AVP might be a potential anti-tumor agent securely and naturally.
28807689	7	47	theme	anti-tumor	1264:1273	arg1	agent					1275:1279	a potential anti-tumor agent	1252:1279	a potential anti-tumor agent	1252:1279	The results suggested that AVP might be a potential anti-tumor agent securely and naturally.
28807689	0	48	theme	Haliotis	49:56	arg1	Ino					72:74	Haliotis discus hannai Ino	49:74	Haliotis discus hannai Ino	49:74	Effects of polysaccharides from abalone viscera (Haliotis discus hannai Ino) on MGC 803 cells proliferation.
28807689	0	48	theme	Haliotis	49:56	arg1	viscera					40:46	abalone viscera	32:46	abalone viscera (Haliotis discus hannai Ino)	32:75	Effects of polysaccharides from abalone viscera (Haliotis discus hannai Ino) on MGC 803 cells proliferation.
28807689	1	49	theme	repeated	261:268	arg1	protease					218:225	the alkaline protease	205:225	the alkaline protease to enzymolysis	205:240	The polysaccharides (AVP) was obtained from abalone (Haliotis discus hannai Ino) viscera, using the alkaline protease to enzymolysis, sevage method and repeated freezing and thawing method to remove protein and hydrogen peroxide method to depigment.
28807689	1	49	theme	repeated	261:268	arg1	method					291:296	repeated freezing and thawing method	261:296	repeated freezing and thawing method	261:296	The polysaccharides (AVP) was obtained from abalone (Haliotis discus hannai Ino) viscera, using the alkaline protease to enzymolysis, sevage method and repeated freezing and thawing method to remove protein and hydrogen peroxide method to depigment.
28807689	2	50	theme	hexosamine	432:441	arg1	contents					455:462	uronic acid, sulfate radical, hexosamine and protein contents	402:462	uronic acid, sulfate radical, hexosamine and protein contents	402:462	The total sugar content was 46.27±1.5% and uronic acid, sulfate radical, hexosamine and protein contents were 17.44±0.22%, 16.98±0.15%, 0.65±0.02% and 1.64±0.13% in AVP respectively.
28807689	1	51	theme	hydrogen	320:327	arg1	peroxide					329:336	hydrogen peroxide	320:336	hydrogen peroxide	320:336	The polysaccharides (AVP) was obtained from abalone (Haliotis discus hannai Ino) viscera, using the alkaline protease to enzymolysis, sevage method and repeated freezing and thawing method to remove protein and hydrogen peroxide method to depigment.
28807689	2	52	theme	uronic	402:407	arg1	acid					409:412	uronic acid	402:412	uronic acid	402:412	The total sugar content was 46.27±1.5% and uronic acid, sulfate radical, hexosamine and protein contents were 17.44±0.22%, 16.98±0.15%, 0.65±0.02% and 1.64±0.13% in AVP respectively.
28807689	1	53	theme	freezing	270:277	arg1	protease					218:225	the alkaline protease	205:225	the alkaline protease to enzymolysis	205:240	The polysaccharides (AVP) was obtained from abalone (Haliotis discus hannai Ino) viscera, using the alkaline protease to enzymolysis, sevage method and repeated freezing and thawing method to remove protein and hydrogen peroxide method to depigment.
28807689	1	53	theme	freezing	270:277	arg1	method					291:296	repeated freezing and thawing method	261:296	repeated freezing and thawing method	261:296	The polysaccharides (AVP) was obtained from abalone (Haliotis discus hannai Ino) viscera, using the alkaline protease to enzymolysis, sevage method and repeated freezing and thawing method to remove protein and hydrogen peroxide method to depigment.
28807689	5	54	theme	MGC803	939:944	arg1	cells					946:950	MGC803 cells	939:950	MGC803 cells	939:950	The results of Morphological changes, cell migration ability and AO/EB staining indicated that MGC803 cells underwent apoptosis in a dose-dependent manner induced by AVP.
28807689	4	55	dep	showed	668:673	arg1	had					679:681	had	679:681	had	679:681	MTT assay showed AVP had a significant anti-tumor activity to gastric carcinoma cells, especially to MGC 803, while it had no influence upon proliferation of normal stomach cells GES 1.
28807689	5	56	theme	AO/EB	909:913	arg1	staining					915:922	AO/EB staining	909:922	AO/EB staining	909:922	The results of Morphological changes, cell migration ability and AO/EB staining indicated that MGC803 cells underwent apoptosis in a dose-dependent manner induced by AVP.
28807689	4	57	theme	normal	816:821	arg1	cells					831:835	normal stomach cells GES 1	816:841	normal stomach cells GES 1	816:841	MTT assay showed AVP had a significant anti-tumor activity to gastric carcinoma cells, especially to MGC 803, while it had no influence upon proliferation of normal stomach cells GES 1.
28807689	5	58	theme	migration	887:895	arg1	ability					897:903	cell migration ability	882:903	cell migration ability	882:903	The results of Morphological changes, cell migration ability and AO/EB staining indicated that MGC803 cells underwent apoptosis in a dose-dependent manner induced by AVP.
28807689	5	59	theme	cell	882:885	arg1	ability					897:903	cell migration ability	882:903	cell migration ability	882:903	The results of Morphological changes, cell migration ability and AO/EB staining indicated that MGC803 cells underwent apoptosis in a dose-dependent manner induced by AVP.
28807689	3	60	theme	main	546:549	arg1	d-galactose					591:601	d-galactose	591:601	d-galactose	591:601	The main monosaccharide compositions of AVP were d-galactose, d-xylose, d-mannose, d-glucose and d-glucuronic acid.
28807689	3	60	theme	main	546:549	arg1	compositions					566:577	The main monosaccharide compositions	542:577	The main monosaccharide compositions of AVP	542:584	The main monosaccharide compositions of AVP were d-galactose, d-xylose, d-mannose, d-glucose and d-glucuronic acid.
28807689	5	61	theme	dose-dependent	977:990	arg1	manner					992:997	a dose-dependent manner	975:997	a dose-dependent manner induced by AVP	975:1012	The results of Morphological changes, cell migration ability and AO/EB staining indicated that MGC803 cells underwent apoptosis in a dose-dependent manner induced by AVP.
28807689	2	62	from	%	479:479	arg1	AVP					524:526	AVP	524:526	AVP	524:526	The total sugar content was 46.27±1.5% and uronic acid, sulfate radical, hexosamine and protein contents were 17.44±0.22%, 16.98±0.15%, 0.65±0.02% and 1.64±0.13% in AVP respectively.
28807689	0	63	theme	hannai	65:70	arg1	Ino					72:74	Haliotis discus hannai Ino	49:74	Haliotis discus hannai Ino	49:74	Effects of polysaccharides from abalone viscera (Haliotis discus hannai Ino) on MGC 803 cells proliferation.
28807689	0	63	theme	hannai	65:70	arg1	viscera					40:46	abalone viscera	32:46	abalone viscera (Haliotis discus hannai Ino)	32:75	Effects of polysaccharides from abalone viscera (Haliotis discus hannai Ino) on MGC 803 cells proliferation.
28807689	4	64	theme	carcinoma	728:736	arg1	cells					738:742	gastric carcinoma cells	720:742	gastric carcinoma cells	720:742	MTT assay showed AVP had a significant anti-tumor activity to gastric carcinoma cells, especially to MGC 803, while it had no influence upon proliferation of normal stomach cells GES 1.
28807689	2	65	from	%	492:492	arg1	AVP					524:526	AVP	524:526	AVP	524:526	The total sugar content was 46.27±1.5% and uronic acid, sulfate radical, hexosamine and protein contents were 17.44±0.22%, 16.98±0.15%, 0.65±0.02% and 1.64±0.13% in AVP respectively.
28807689	0	66	theme	discus	58:63	arg1	Ino					72:74	Haliotis discus hannai Ino	49:74	Haliotis discus hannai Ino	49:74	Effects of polysaccharides from abalone viscera (Haliotis discus hannai Ino) on MGC 803 cells proliferation.
28807689	0	66	theme	discus	58:63	arg1	viscera					40:46	abalone viscera	32:46	abalone viscera (Haliotis discus hannai Ino)	32:75	Effects of polysaccharides from abalone viscera (Haliotis discus hannai Ino) on MGC 803 cells proliferation.
28807689	1	67	theme	thawing	283:289	arg1	protease					218:225	the alkaline protease	205:225	the alkaline protease to enzymolysis	205:240	The polysaccharides (AVP) was obtained from abalone (Haliotis discus hannai Ino) viscera, using the alkaline protease to enzymolysis, sevage method and repeated freezing and thawing method to remove protein and hydrogen peroxide method to depigment.
28807689	1	67	theme	thawing	283:289	arg1	method					291:296	repeated freezing and thawing method	261:296	repeated freezing and thawing method	261:296	The polysaccharides (AVP) was obtained from abalone (Haliotis discus hannai Ino) viscera, using the alkaline protease to enzymolysis, sevage method and repeated freezing and thawing method to remove protein and hydrogen peroxide method to depigment.
28807689	4	68	theme	gastric	720:726	arg1	cells					738:742	gastric carcinoma cells	720:742	gastric carcinoma cells	720:742	MTT assay showed AVP had a significant anti-tumor activity to gastric carcinoma cells, especially to MGC 803, while it had no influence upon proliferation of normal stomach cells GES 1.
28024535	6	0	theme	other	536:540	arg1	amylopectins					542:553	other amylopectins	536:553	other amylopectins	536:553	Compared with other amylopectins, quinoa amylopectin showed a high ratio of short chain to long chains (mean:14.6) and a high percentage of fingerprint A-chains (Afp) (mean:10.4%).
28024535	3	1	theme	amylopectin	206:216	arg1	structure					228:236	amylopectin molecular structure	206:236	amylopectin molecular structure	206:236	In this study, composition and amylopectin molecular structure of 9 quinoa starch samples were characterised by chromatographic techniques.
28024535	8	2	theme	component	893:901	arg1	analysis					903:910	principal component analysis	883:910	principal component analysis	883:910	Pearson correlation and principal component analysis revealed some inherent correlations among structural parameters and a similarity of different samples.
28024535	6	3	theme	long	613:616	arg1	chains					618:623	long chains	613:623	long chains	613:623	Compared with other amylopectins, quinoa amylopectin showed a high ratio of short chain to long chains (mean:14.6) and a high percentage of fingerprint A-chains (Afp) (mean:10.4%).
28024535	3	4	theme	molecular	218:226	arg1	structure					228:236	amylopectin molecular structure	206:236	amylopectin molecular structure	206:236	In this study, composition and amylopectin molecular structure of 9 quinoa starch samples were characterised by chromatographic techniques.
28024535	4	5	theme	β-limit	394:400	arg1	LDs					412:414	LDs	412:414	LDs	412:414	In particular, the amylopectin internal molecular structure, represented by φ, β-limit dextrins (LDs), was explored.
28024535	4	5	theme	β-limit	394:400	arg1	φ					391:391	φ	391:391	φ	391:391	In particular, the amylopectin internal molecular structure, represented by φ, β-limit dextrins (LDs), was explored.
28024535	4	5	theme	β-limit	394:400	arg1	dextrins					402:409	β-limit dextrins	394:409	β-limit dextrins (LDs)	394:415	In particular, the amylopectin internal molecular structure, represented by φ, β-limit dextrins (LDs), was explored.
28024535	8	6	theme	different	996:1004	arg1	samples					1006:1012	different samples	996:1012	different samples	996:1012	Pearson correlation and principal component analysis revealed some inherent correlations among structural parameters and a similarity of different samples.
28024535	6	7	theme	chain	604:608	arg1	percentage					648:657	a high percentage	641:657	a high percentage of fingerprint A-chains (Afp) (mean:10.4%)	641:700	Compared with other amylopectins, quinoa amylopectin showed a high ratio of short chain to long chains (mean:14.6) and a high percentage of fingerprint A-chains (Afp) (mean:10.4%).
28024535	6	7	theme	chain	604:608	arg1	ratio					589:593	a high ratio	582:593	a high ratio of short chain to long chains (mean:14.6)	582:635	Compared with other amylopectins, quinoa amylopectin showed a high ratio of short chain to long chains (mean:14.6) and a high percentage of fingerprint A-chains (Afp) (mean:10.4%).
28024535	6	7	theme	chain	604:608	arg1	%					699:699	mean:10.4%	690:699	mean:10.4%	690:699	Compared with other amylopectins, quinoa amylopectin showed a high ratio of short chain to long chains (mean:14.6) and a high percentage of fingerprint A-chains (Afp) (mean:10.4%).
28024535	6	7	theme	chain	604:608	arg1	mean:14.6					626:634	mean:14.6	626:634	mean:14.6	626:634	Compared with other amylopectins, quinoa amylopectin showed a high ratio of short chain to long chains (mean:14.6) and a high percentage of fingerprint A-chains (Afp) (mean:10.4%).
28024535	9	8	with	starches	1082:1089	arg1	polymorph					1103:1111	A-type polymorph	1096:1111	A-type polymorph	1096:1111	Overall, quinoa amylopectins are structurally similar to that from starches with A-type polymorph such as oat and amaranth starches.
28024535	8	9	theme	principal	883:891	arg1	analysis					903:910	principal component analysis	883:910	principal component analysis	883:910	Pearson correlation and principal component analysis revealed some inherent correlations among structural parameters and a similarity of different samples.
28024535	2	10	theme	starch	143:148	arg1	structure					123:131	the molecular structure	109:131	the molecular structure of quinoa starch	109:148	However, the molecular structure of quinoa starch remains largely unknown.
28024535	8	11	theme	Pearson	859:865	arg1	correlation					867:877	Pearson correlation	859:877	Pearson correlation	859:877	Pearson correlation and principal component analysis revealed some inherent correlations among structural parameters and a similarity of different samples.
28024535	7	12	theme	internal	756:763	arg1	length					771:776	internal chain length	756:776	internal chain length	756:776	The average chain length, external chain length, and internal chain length of quinoa amylopectin were 16.6, 10.6, and 5.00 glucosyl residues, respectively.
28024535	5	13	theme	molecular	472:480	arg1	structures					482:491	molecular structures	472:491	molecular structures	472:491	Great variations in the composition and molecular structures were recorded among samples.
28024535	7	14	theme	amylopectin	788:798	arg1	length					771:776	internal chain length	756:776	internal chain length	756:776	The average chain length, external chain length, and internal chain length of quinoa amylopectin were 16.6, 10.6, and 5.00 glucosyl residues, respectively.
28024535	7	14	theme	amylopectin	788:798	arg1	length					744:749	external chain length	729:749	external chain length	729:749	The average chain length, external chain length, and internal chain length of quinoa amylopectin were 16.6, 10.6, and 5.00 glucosyl residues, respectively.
28024535	7	14	theme	amylopectin	788:798	arg1	residues					835:842	16.6, 10.6, and 5.00 glucosyl residues	805:842	16.6, 10.6, and 5.00 glucosyl residues	805:842	The average chain length, external chain length, and internal chain length of quinoa amylopectin were 16.6, 10.6, and 5.00 glucosyl residues, respectively.
28024535	7	14	theme	amylopectin	788:798	arg1	length					721:726	The average chain length	703:726	The average chain length	703:726	The average chain length, external chain length, and internal chain length of quinoa amylopectin were 16.6, 10.6, and 5.00 glucosyl residues, respectively.
28024535	2	15	theme	quinoa	136:141	arg1	starch					143:148	quinoa starch	136:148	quinoa starch	136:148	However, the molecular structure of quinoa starch remains largely unknown.
28024535	8	16	theme	inherent	926:933	arg1	correlations					935:946	some inherent correlations	921:946	some inherent correlations among structural parameters	921:974	Pearson correlation and principal component analysis revealed some inherent correlations among structural parameters and a similarity of different samples.
28024535	1	17	theme	small	61:65	arg1	granules					67:74	very small granules	56:74	very small granules	56:74	Quinoa starch has very small granules with unique properties.
28024535	8	18	theme	samples	1006:1012	arg1	correlations					935:946	some inherent correlations	921:946	some inherent correlations among structural parameters	921:974	Pearson correlation and principal component analysis revealed some inherent correlations among structural parameters and a similarity of different samples.
28024535	8	18	theme	samples	1006:1012	arg1	similarity					982:991	a similarity	980:991	a similarity of different samples	980:1012	Pearson correlation and principal component analysis revealed some inherent correlations among structural parameters and a similarity of different samples.
28024535	8	19	theme	structural	954:963	arg1	parameters					965:974	structural parameters	954:974	structural parameters	954:974	Pearson correlation and principal component analysis revealed some inherent correlations among structural parameters and a similarity of different samples.
28024535	7	20	theme	average	707:713	arg1	residues					835:842	16.6, 10.6, and 5.00 glucosyl residues	805:842	16.6, 10.6, and 5.00 glucosyl residues	805:842	The average chain length, external chain length, and internal chain length of quinoa amylopectin were 16.6, 10.6, and 5.00 glucosyl residues, respectively.
28024535	7	20	theme	average	707:713	arg1	length					721:726	The average chain length	703:726	The average chain length	703:726	The average chain length, external chain length, and internal chain length of quinoa amylopectin were 16.6, 10.6, and 5.00 glucosyl residues, respectively.
28024535	6	21	theme	A-chains	674:681	arg1	percentage					648:657	a high percentage	641:657	a high percentage of fingerprint A-chains (Afp) (mean:10.4%)	641:700	Compared with other amylopectins, quinoa amylopectin showed a high ratio of short chain to long chains (mean:14.6) and a high percentage of fingerprint A-chains (Afp) (mean:10.4%).
28024535	6	21	theme	A-chains	674:681	arg1	ratio					589:593	a high ratio	582:593	a high ratio of short chain to long chains (mean:14.6)	582:635	Compared with other amylopectins, quinoa amylopectin showed a high ratio of short chain to long chains (mean:14.6) and a high percentage of fingerprint A-chains (Afp) (mean:10.4%).
28024535	6	21	theme	A-chains	674:681	arg1	%					699:699	mean:10.4%	690:699	mean:10.4%	690:699	Compared with other amylopectins, quinoa amylopectin showed a high ratio of short chain to long chains (mean:14.6) and a high percentage of fingerprint A-chains (Afp) (mean:10.4%).
28024535	6	21	theme	A-chains	674:681	arg1	mean:14.6					626:634	mean:14.6	626:634	mean:14.6	626:634	Compared with other amylopectins, quinoa amylopectin showed a high ratio of short chain to long chains (mean:14.6) and a high percentage of fingerprint A-chains (Afp) (mean:10.4%).
28024535	6	22	theme	short	598:602	arg1	chain					604:608	short chain	598:608	short chain	598:608	Compared with other amylopectins, quinoa amylopectin showed a high ratio of short chain to long chains (mean:14.6) and a high percentage of fingerprint A-chains (Afp) (mean:10.4%).
28024535	6	23	theme	fingerprint	662:672	arg1	A-chains					674:681	fingerprint A-chains	662:681	fingerprint A-chains (Afp)	662:687	Compared with other amylopectins, quinoa amylopectin showed a high ratio of short chain to long chains (mean:14.6) and a high percentage of fingerprint A-chains (Afp) (mean:10.4%).
28024535	6	23	theme	fingerprint	662:672	arg1	Afp					684:686	Afp	684:686	Afp	684:686	Compared with other amylopectins, quinoa amylopectin showed a high ratio of short chain to long chains (mean:14.6) and a high percentage of fingerprint A-chains (Afp) (mean:10.4%).
28024535	0	24	theme	Molecular	0:8	arg1	structure					10:18	Molecular structure	0:18	Molecular structure of quinoa starch	0:35	Molecular structure of quinoa starch.
28024535	1	25	theme	unique	81:86	arg1	properties					88:97	unique properties	81:97	unique properties	81:97	Quinoa starch has very small granules with unique properties.
28024535	0	26	theme	quinoa	23:28	arg1	starch					30:35	quinoa starch	23:35	quinoa starch	23:35	Molecular structure of quinoa starch.
28024535	9	27	theme	amaranth	1129:1136	arg1	starches					1138:1145	oat and amaranth starches	1121:1145	starches	1138:1145	Overall, quinoa amylopectins are structurally similar to that from starches with A-type polymorph such as oat and amaranth starches.
28024535	5	28	from	variations	438:447	arg1	composition					456:466	composition	456:466	composition	456:466	Great variations in the composition and molecular structures were recorded among samples.
28024535	5	28	from	variations	438:447	arg1	structures					482:491	molecular structures	472:491	molecular structures	472:491	Great variations in the composition and molecular structures were recorded among samples.
28024535	7	29	theme	chain	738:742	arg1	length					744:749	external chain length	729:749	external chain length	729:749	The average chain length, external chain length, and internal chain length of quinoa amylopectin were 16.6, 10.6, and 5.00 glucosyl residues, respectively.
28024535	6	30	theme	high	643:646	arg1	percentage					648:657	a high percentage	641:657	a high percentage of fingerprint A-chains (Afp) (mean:10.4%)	641:700	Compared with other amylopectins, quinoa amylopectin showed a high ratio of short chain to long chains (mean:14.6) and a high percentage of fingerprint A-chains (Afp) (mean:10.4%).
28024535	6	30	theme	high	643:646	arg1	%					699:699	mean:10.4%	690:699	mean:10.4%	690:699	Compared with other amylopectins, quinoa amylopectin showed a high ratio of short chain to long chains (mean:14.6) and a high percentage of fingerprint A-chains (Afp) (mean:10.4%).
28024535	1	31	contain	has	52:54	arg2	granules					67:74	very small granules	56:74	very small granules	56:74	Quinoa starch has very small granules with unique properties.
28024535	1	31	contain	has	52:54	arg1	starch					45:50	Quinoa starch	38:50	Quinoa starch	38:50	Quinoa starch has very small granules with unique properties.
28024535	3	32	theme	starch	250:255	arg1	samples					257:263	9 quinoa starch samples	241:263	9 quinoa starch samples	241:263	In this study, composition and amylopectin molecular structure of 9 quinoa starch samples were characterised by chromatographic techniques.
28024535	7	33	theme	chain	765:769	arg1	length					771:776	internal chain length	756:776	internal chain length	756:776	The average chain length, external chain length, and internal chain length of quinoa amylopectin were 16.6, 10.6, and 5.00 glucosyl residues, respectively.
28024535	0	34	theme	starch	30:35	arg1	structure					10:18	Molecular structure	0:18	Molecular structure of quinoa starch	0:35	Molecular structure of quinoa starch.
28024535	3	35	theme	samples	257:263	arg1	composition					190:200	composition	190:200	composition	190:200	In this study, composition and amylopectin molecular structure of 9 quinoa starch samples were characterised by chromatographic techniques.
28024535	3	35	theme	samples	257:263	arg1	structure					228:236	amylopectin molecular structure	206:236	amylopectin molecular structure	206:236	In this study, composition and amylopectin molecular structure of 9 quinoa starch samples were characterised by chromatographic techniques.
28024535	4	36	theme	internal	346:353	arg1	structure					365:373	the amylopectin internal molecular structure	330:373	the amylopectin internal molecular structure	330:373	In particular, the amylopectin internal molecular structure, represented by φ, β-limit dextrins (LDs), was explored.
28024535	7	37	theme	external	729:736	arg1	length					744:749	external chain length	729:749	external chain length	729:749	The average chain length, external chain length, and internal chain length of quinoa amylopectin were 16.6, 10.6, and 5.00 glucosyl residues, respectively.
28024535	4	38	theme	amylopectin	334:344	arg1	structure					365:373	the amylopectin internal molecular structure	330:373	the amylopectin internal molecular structure	330:373	In particular, the amylopectin internal molecular structure, represented by φ, β-limit dextrins (LDs), was explored.
28024535	6	39	theme	quinoa	556:561	arg1	amylopectin					563:573	quinoa amylopectin	556:573	quinoa amylopectin	556:573	Compared with other amylopectins, quinoa amylopectin showed a high ratio of short chain to long chains (mean:14.6) and a high percentage of fingerprint A-chains (Afp) (mean:10.4%).
28024535	7	40	theme	chain	715:719	arg1	residues					835:842	16.6, 10.6, and 5.00 glucosyl residues	805:842	16.6, 10.6, and 5.00 glucosyl residues	805:842	The average chain length, external chain length, and internal chain length of quinoa amylopectin were 16.6, 10.6, and 5.00 glucosyl residues, respectively.
28024535	7	40	theme	chain	715:719	arg1	length					721:726	The average chain length	703:726	The average chain length	703:726	The average chain length, external chain length, and internal chain length of quinoa amylopectin were 16.6, 10.6, and 5.00 glucosyl residues, respectively.
28024535	9	41	theme	A-type	1096:1101	arg1	polymorph					1103:1111	A-type polymorph	1096:1111	A-type polymorph	1096:1111	Overall, quinoa amylopectins are structurally similar to that from starches with A-type polymorph such as oat and amaranth starches.
28024535	4	42	theme	molecular	355:363	arg1	structure					365:373	the amylopectin internal molecular structure	330:373	the amylopectin internal molecular structure	330:373	In particular, the amylopectin internal molecular structure, represented by φ, β-limit dextrins (LDs), was explored.
28024535	3	43	theme	chromatographic	287:301	arg1	techniques					303:312	chromatographic techniques	287:312	chromatographic techniques	287:312	In this study, composition and amylopectin molecular structure of 9 quinoa starch samples were characterised by chromatographic techniques.
28024535	5	44	dep	composition	456:466	arg1	the					452:454	the	452:454	the	452:454	Great variations in the composition and molecular structures were recorded among samples.
28024535	5	45	theme	Great	432:436	arg1	variations					438:447	Great variations	432:447	Great variations in the composition and molecular structures	432:491	Great variations in the composition and molecular structures were recorded among samples.
28024535	7	46	theme	quinoa	781:786	arg1	amylopectin					788:798	quinoa amylopectin	781:798	quinoa amylopectin	781:798	The average chain length, external chain length, and internal chain length of quinoa amylopectin were 16.6, 10.6, and 5.00 glucosyl residues, respectively.
28024535	1	47	theme	Quinoa	38:43	arg1	starch					45:50	Quinoa starch	38:50	Quinoa starch	38:50	Quinoa starch has very small granules with unique properties.
28024535	7	48	theme	glucosyl	826:833	arg1	residues					835:842	16.6, 10.6, and 5.00 glucosyl residues	805:842	16.6, 10.6, and 5.00 glucosyl residues	805:842	The average chain length, external chain length, and internal chain length of quinoa amylopectin were 16.6, 10.6, and 5.00 glucosyl residues, respectively.
28024535	7	48	theme	glucosyl	826:833	arg1	length					721:726	The average chain length	703:726	The average chain length	703:726	The average chain length, external chain length, and internal chain length of quinoa amylopectin were 16.6, 10.6, and 5.00 glucosyl residues, respectively.
28024535	2	49	theme	molecular	113:121	arg1	structure					123:131	the molecular structure	109:131	the molecular structure of quinoa starch	109:148	However, the molecular structure of quinoa starch remains largely unknown.
28024535	6	50	theme	high	584:587	arg1	ratio					589:593	a high ratio	582:593	a high ratio of short chain to long chains (mean:14.6)	582:635	Compared with other amylopectins, quinoa amylopectin showed a high ratio of short chain to long chains (mean:14.6) and a high percentage of fingerprint A-chains (Afp) (mean:10.4%).
28024535	6	50	theme	high	584:587	arg1	mean:14.6					626:634	mean:14.6	626:634	mean:14.6	626:634	Compared with other amylopectins, quinoa amylopectin showed a high ratio of short chain to long chains (mean:14.6) and a high percentage of fingerprint A-chains (Afp) (mean:10.4%).
28024535	9	51	theme	oat	1121:1123	arg1	starches					1138:1145	oat and amaranth starches	1121:1145	starches	1138:1145	Overall, quinoa amylopectins are structurally similar to that from starches with A-type polymorph such as oat and amaranth starches.
28024535	9	52	theme	quinoa	1024:1029	arg1	amylopectins					1031:1042	quinoa amylopectins	1024:1042	quinoa amylopectins	1024:1042	Overall, quinoa amylopectins are structurally similar to that from starches with A-type polymorph such as oat and amaranth starches.
27134013	2	0	theme	thiol	615:619	arg1	groups					641:646	thiol and 2-dithiopyridyl groups	615:646	thiol and 2-dithiopyridyl groups	615:646	The synthetic pathway included the preparation of mutually reactive HA derivatives bearing thiol and 2-dithiopyridyl groups.
27134013	4	1	theme	reference	962:970	arg1	experiments					972:982	reference experiments	962:982	reference experiments	962:982	The formation of two networks was confirmed by conducting reference experiments with the compositions lacking one of the four components.
27134013	8	2	theme	receptor	1609:1616	arg1	CD44					1618:1621	the hyaluronan receptor CD44	1594:1621	the hyaluronan receptor CD44 in IPN hydrogel	1594:1637	Moreover, the proliferation and spreading of MG63 cells that express the hyaluronan receptor CD44 in IPN hydrogel was increased, as compared with its single network analogues.
27134013	10	3	theme	extracellular	1886:1898	arg1	hydrogels					1907:1915	injectable extracellular matrix hydrogels	1875:1915	injectable extracellular matrix hydrogels	1875:1915	STATEMENT OF SIGNIFICANCE The present work is devoted to in situ fabrication of injectable extracellular matrix hydrogels through simultaneous generation of networks of fibrin and hyaluronic acid (HA) that interpenetrate each other.
27134013	13	4	theme	lower	2373:2377	arg1	rate					2391:2394	a lower degradation rate	2371:2394	a lower degradation rate of fibrin in comparison to the single fibrin network	2371:2447	The interpenetrating network hydrogel shows an increased stiffness along with a lower degradation rate of fibrin in comparison to the single fibrin network.
27134013	1	5	theme	engineering	279:289	arg1	applications					296:307	tissue engineering (TE) applications	272:307	tissue engineering (TE) applications	272:307	UNLABELLED To strengthen the mechanical properties of a fibrin gel and improve its applicability as a scaffold for tissue engineering (TE) applications, a strategy for the in situ preparation of the interpenetrating network (IPN) of fibrin and hyaluronic acid (HA) was developed on the basis of simultaneous and orthogonal fibrinogenesis and disulfide cross-linking.
27134013	14	6	theme	sufficient	2478:2487	arg1	time					2489:2492	sufficient time	2478:2492	sufficient time	2478:2492	As a result, the cells have sufficient time for the remodelling of the scaffold.
27134013	14	6	theme	sufficient	2478:2487	arg1	result					2455:2460	a result	2453:2460	a result	2453:2460	As a result, the cells have sufficient time for the remodelling of the scaffold.
27134013	2	7	theme	derivatives	595:605	arg1	preparation					559:569	the preparation	555:569	the preparation of mutually reactive HA derivatives bearing thiol and 2-dithiopyridyl groups	555:646	The synthetic pathway included the preparation of mutually reactive HA derivatives bearing thiol and 2-dithiopyridyl groups.
27134013	15	8	theme	engineering	2626:2636	arg1	scaffolds					2638:2646	tissue engineering scaffolds	2619:2646	tissue engineering scaffolds	2619:2646	This new approach can be applied for modular construction of in vitro tissue models and tissue engineering scaffolds in vivo.
27134013	5	9	dep	%	1082:1082	arg1	w/v					1085:1087	w/v	1085:1087	w/v	1085:1087	The composition of 2% (w/v) fibrin and 1% (w/v) HA showed the highest storage modulus (G'), as compared with the single network counterparts.
27134013	3	10	theme	obtained	762:769	arg1	formulations					778:789	the obtained liquid formulations	758:789	the obtained liquid formulations	758:789	Combining thiol-derivatized HA with thrombin and 2-dithiopyridyl-modified HA with fibrinogen and then mixing the obtained liquid formulations afforded IPNs with fibrin-resembling fibrillar architectures at different ratios between fibrin and HA networks.
27134013	6	11	theme	fibrin	1256:1261	arg1	gels					1263:1266	pure fibrin gels	1251:1266	pure fibrin gels	1251:1266	The degradation of fibrin in IPN hydrogels was slower than that in pure fibrin gels both during incubation of the hydrogels in a fibrin-cleaving nattokinase solution and during the culturing of cells after their encapsulation in the hydrogels.
27134013	8	12	theme	single	1675:1680	arg1	analogues					1690:1698	its single network analogues	1671:1698	its single network analogues	1671:1698	Moreover, the proliferation and spreading of MG63 cells that express the hyaluronan receptor CD44 in IPN hydrogel was increased, as compared with its single network analogues.
27134013	7	13	theme	HA	1461:1462	arg1	network					1464:1470	HA network	1461:1470	HA network	1461:1470	Together with the persistence of HA network, it permitted longer cell culturing time in the IPN.
27134013	10	14	theme	simultaneous	1925:1936	arg1	generation					1938:1947	simultaneous generation	1925:1947	simultaneous generation of networks of fibrin	1925:1969	STATEMENT OF SIGNIFICANCE The present work is devoted to in situ fabrication of injectable extracellular matrix hydrogels through simultaneous generation of networks of fibrin and hyaluronic acid (HA) that interpenetrate each other.
27134013	7	15	dep	longer	1486:1491	arg1	time					1508:1511	cell culturing time	1493:1511	cell culturing time	1493:1511	Together with the persistence of HA network, it permitted longer cell culturing time in the IPN.
27134013	15	16	theme	tissue	2619:2624	arg1	scaffolds					2638:2646	tissue engineering scaffolds	2619:2646	tissue engineering scaffolds	2619:2646	This new approach can be applied for modular construction of in vitro tissue models and tissue engineering scaffolds in vivo.
27134013	2	17	theme	2-dithiopyridyl	625:639	arg1	groups					641:646	thiol and 2-dithiopyridyl groups	615:646	thiol and 2-dithiopyridyl groups	615:646	The synthetic pathway included the preparation of mutually reactive HA derivatives bearing thiol and 2-dithiopyridyl groups.
27134013	5	18	theme	%	1082:1082	arg1	HA					1090:1091	1% (w/v) HA	1081:1091	1% (w/v) HA	1081:1091	The composition of 2% (w/v) fibrin and 1% (w/v) HA showed the highest storage modulus (G'), as compared with the single network counterparts.
27134013	15	19	theme	models	2608:2613	arg1	construction					2576:2587	modular construction	2568:2587	modular construction of in vitro tissue models and tissue engineering scaffolds	2568:2646	This new approach can be applied for modular construction of in vitro tissue models and tissue engineering scaffolds in vivo.
27134013	1	20	theme	gel	220:222	arg1	properties					197:206	the mechanical properties	182:206	the mechanical properties of a fibrin gel	182:222	UNLABELLED To strengthen the mechanical properties of a fibrin gel and improve its applicability as a scaffold for tissue engineering (TE) applications, a strategy for the in situ preparation of the interpenetrating network (IPN) of fibrin and hyaluronic acid (HA) was developed on the basis of simultaneous and orthogonal fibrinogenesis and disulfide cross-linking.
27134013	11	21	theme	HA	2142:2143	arg1	cross-linking					2125:2137	orthogonal disulphide cross-linking	2103:2137	orthogonal disulphide cross-linking of HA	2103:2143	This is accomplished by combination of enzymatic fibrin cross-linking with orthogonal disulphide cross-linking of HA.
27134013	15	22	theme	scaffolds	2638:2646	arg1	construction					2576:2587	modular construction	2568:2587	modular construction of in vitro tissue models and tissue engineering scaffolds	2568:2646	This new approach can be applied for modular construction of in vitro tissue models and tissue engineering scaffolds in vivo.
27134013	6	23	theme	fibrin-cleaving	1313:1327	arg1	solution					1341:1348	a fibrin-cleaving nattokinase solution	1311:1348	a fibrin-cleaving nattokinase solution	1311:1348	The degradation of fibrin in IPN hydrogels was slower than that in pure fibrin gels both during incubation of the hydrogels in a fibrin-cleaving nattokinase solution and during the culturing of cells after their encapsulation in the hydrogels.
27134013	5	24	theme	HA	1090:1091	arg1	composition					1046:1056	The composition	1042:1056	The composition of 2% (w/v) fibrin and 1% (w/v) HA	1042:1091	The composition of 2% (w/v) fibrin and 1% (w/v) HA showed the highest storage modulus (G'), as compared with the single network counterparts.
27134013	3	25	with	HA	677:678	arg1	fibrinogen					731:740	fibrinogen	731:740	fibrinogen	731:740	Combining thiol-derivatized HA with thrombin and 2-dithiopyridyl-modified HA with fibrinogen and then mixing the obtained liquid formulations afforded IPNs with fibrin-resembling fibrillar architectures at different ratios between fibrin and HA networks.
27134013	3	25	with	HA	677:678	arg1	thrombin					685:692	thrombin	685:692	thrombin	685:692	Combining thiol-derivatized HA with thrombin and 2-dithiopyridyl-modified HA with fibrinogen and then mixing the obtained liquid formulations afforded IPNs with fibrin-resembling fibrillar architectures at different ratios between fibrin and HA networks.
27134013	1	26	theme	fibrin	390:395	arg1	network					373:379	the interpenetrating network	352:379	the interpenetrating network (IPN) of fibrin and hyaluronic acid (HA)	352:420	UNLABELLED To strengthen the mechanical properties of a fibrin gel and improve its applicability as a scaffold for tissue engineering (TE) applications, a strategy for the in situ preparation of the interpenetrating network (IPN) of fibrin and hyaluronic acid (HA) was developed on the basis of simultaneous and orthogonal fibrinogenesis and disulfide cross-linking.
27134013	1	26	theme	fibrin	390:395	arg1	IPN					382:384	IPN	382:384	IPN	382:384	UNLABELLED To strengthen the mechanical properties of a fibrin gel and improve its applicability as a scaffold for tissue engineering (TE) applications, a strategy for the in situ preparation of the interpenetrating network (IPN) of fibrin and hyaluronic acid (HA) was developed on the basis of simultaneous and orthogonal fibrinogenesis and disulfide cross-linking.
27134013	10	27	theme	hyaluronic	1975:1984	arg1	acid					1986:1989	hyaluronic acid	1975:1989	hyaluronic acid (HA)	1975:1994	STATEMENT OF SIGNIFICANCE The present work is devoted to in situ fabrication of injectable extracellular matrix hydrogels through simultaneous generation of networks of fibrin and hyaluronic acid (HA) that interpenetrate each other.
27134013	10	27	theme	hyaluronic	1975:1984	arg1	HA					1992:1993	HA	1992:1993	HA	1992:1993	STATEMENT OF SIGNIFICANCE The present work is devoted to in situ fabrication of injectable extracellular matrix hydrogels through simultaneous generation of networks of fibrin and hyaluronic acid (HA) that interpenetrate each other.
27134013	2	28	theme	reactive	583:590	arg1	derivatives					595:605	mutually reactive HA derivatives	574:605	mutually reactive HA derivatives bearing thiol and 2-dithiopyridyl groups	574:646	The synthetic pathway included the preparation of mutually reactive HA derivatives bearing thiol and 2-dithiopyridyl groups.
27134013	3	29	theme	fibrin-resembling	810:826	arg1	architectures					838:850	fibrin-resembling fibrillar architectures	810:850	fibrin-resembling fibrillar architectures	810:850	Combining thiol-derivatized HA with thrombin and 2-dithiopyridyl-modified HA with fibrinogen and then mixing the obtained liquid formulations afforded IPNs with fibrin-resembling fibrillar architectures at different ratios between fibrin and HA networks.
27134013	8	30	from	CD44	1618:1621	arg1	hydrogel					1630:1637	IPN hydrogel	1626:1637	IPN hydrogel	1626:1637	Moreover, the proliferation and spreading of MG63 cells that express the hyaluronan receptor CD44 in IPN hydrogel was increased, as compared with its single network analogues.
27134013	6	31	from	encapsulation	1396:1408	arg1	hydrogels					1417:1425	the hydrogels	1413:1425	the hydrogels	1413:1425	The degradation of fibrin in IPN hydrogels was slower than that in pure fibrin gels both during incubation of the hydrogels in a fibrin-cleaving nattokinase solution and during the culturing of cells after their encapsulation in the hydrogels.
27134013	11	32	theme	orthogonal	2103:2112	arg1	cross-linking					2125:2137	orthogonal disulphide cross-linking	2103:2137	orthogonal disulphide cross-linking of HA	2103:2143	This is accomplished by combination of enzymatic fibrin cross-linking with orthogonal disulphide cross-linking of HA.
27134013	11	33	theme	enzymatic	2067:2075	arg1	cross-linking					2084:2096	enzymatic fibrin cross-linking	2067:2096	enzymatic fibrin cross-linking	2067:2096	This is accomplished by combination of enzymatic fibrin cross-linking with orthogonal disulphide cross-linking of HA.
27134013	13	34	theme	network	2314:2320	arg1	hydrogel					2322:2329	The interpenetrating network hydrogel	2293:2329	The interpenetrating network hydrogel	2293:2329	The interpenetrating network hydrogel shows an increased stiffness along with a lower degradation rate of fibrin in comparison to the single fibrin network.
27134013	3	35	theme	different	855:863	arg1	ratios					865:870	different ratios	855:870	different ratios between fibrin and HA networks	855:901	Combining thiol-derivatized HA with thrombin and 2-dithiopyridyl-modified HA with fibrinogen and then mixing the obtained liquid formulations afforded IPNs with fibrin-resembling fibrillar architectures at different ratios between fibrin and HA networks.
27134013	6	36	theme	cells	1378:1382	arg1	culturing					1365:1373	the culturing	1361:1373	the culturing of cells	1361:1382	The degradation of fibrin in IPN hydrogels was slower than that in pure fibrin gels both during incubation of the hydrogels in a fibrin-cleaving nattokinase solution and during the culturing of cells after their encapsulation in the hydrogels.
27134013	15	37	theme	tissue	2601:2606	arg1	models					2608:2613	in vitro tissue models	2592:2613	in vitro tissue models	2592:2613	This new approach can be applied for modular construction of in vitro tissue models and tissue engineering scaffolds in vivo.
27134013	9	38	theme	tunable	1733:1739	arg1	materials					1751:1759	tunable ECM-based materials	1733:1759	tunable ECM-based materials for TE and regenerative medicine	1733:1792	These results are promising for tunable ECM-based materials for TE and regenerative medicine.
27134013	6	39	from	degradation	1188:1198	arg1	hydrogels					1217:1225	IPN hydrogels	1213:1225	IPN hydrogels	1213:1225	The degradation of fibrin in IPN hydrogels was slower than that in pure fibrin gels both during incubation of the hydrogels in a fibrin-cleaving nattokinase solution and during the culturing of cells after their encapsulation in the hydrogels.
27134013	0	40	theme	orthogonal	84:93	arg1	reaction					119:126	orthogonal disulfide cross-linking reaction	84:126	orthogonal disulfide cross-linking reaction for biomedical applications	84:154	Hyaluronic acid-fibrin interpenetrating double network hydrogel prepared in situ by orthogonal disulfide cross-linking reaction for biomedical applications.
27134013	15	41	theme	new	2536:2538	arg1	approach					2540:2547	This new approach	2531:2547	This new approach	2531:2547	This new approach can be applied for modular construction of in vitro tissue models and tissue engineering scaffolds in vivo.
27134013	11	42	theme	disulphide	2114:2123	arg1	cross-linking					2125:2137	orthogonal disulphide cross-linking	2103:2137	orthogonal disulphide cross-linking of HA	2103:2143	This is accomplished by combination of enzymatic fibrin cross-linking with orthogonal disulphide cross-linking of HA.
27134013	12	43	theme	adhesive	2240:2247	arg1	environment					2249:2259	an adhesive environment	2237:2259	an adhesive environment for in situ encapsulated cells	2237:2290	High hydrophilicity of HA prevents compaction of the fibrin network, while fibrin provides an adhesive environment for in situ encapsulated cells.
27134013	6	44	from	fibrin	1203:1208	arg1	hydrogels					1217:1225	IPN hydrogels	1213:1225	IPN hydrogels	1213:1225	The degradation of fibrin in IPN hydrogels was slower than that in pure fibrin gels both during incubation of the hydrogels in a fibrin-cleaving nattokinase solution and during the culturing of cells after their encapsulation in the hydrogels.
27134013	0	45	theme	cross-linking	105:117	arg1	reaction					119:126	orthogonal disulfide cross-linking reaction	84:126	orthogonal disulfide cross-linking reaction for biomedical applications	84:154	Hyaluronic acid-fibrin interpenetrating double network hydrogel prepared in situ by orthogonal disulfide cross-linking reaction for biomedical applications.
27134013	5	46	theme	storage	1112:1118	arg1	G					1129:1129	G'	1129:1130	G'	1129:1130	The composition of 2% (w/v) fibrin and 1% (w/v) HA showed the highest storage modulus (G'), as compared with the single network counterparts.
27134013	5	46	theme	storage	1112:1118	arg1	modulus					1120:1126	the highest storage modulus	1100:1126	the highest storage modulus (G')	1100:1131	The composition of 2% (w/v) fibrin and 1% (w/v) HA showed the highest storage modulus (G'), as compared with the single network counterparts.
27134013	4	47	theme	components	1030:1039	arg1	components					1030:1039	the four components	1021:1039	the four components	1021:1039	The formation of two networks was confirmed by conducting reference experiments with the compositions lacking one of the four components.
27134013	4	47	theme	components	1030:1039	arg1	one					1014:1016	one	1014:1016	one	1014:1016	The formation of two networks was confirmed by conducting reference experiments with the compositions lacking one of the four components.
27134013	1	48	dep	in	329:330	arg1	situ					332:335	situ	332:335	situ	332:335	UNLABELLED To strengthen the mechanical properties of a fibrin gel and improve its applicability as a scaffold for tissue engineering (TE) applications, a strategy for the in situ preparation of the interpenetrating network (IPN) of fibrin and hyaluronic acid (HA) was developed on the basis of simultaneous and orthogonal fibrinogenesis and disulfide cross-linking.
27134013	9	49	theme	regenerative	1772:1783	arg1	medicine					1785:1792	regenerative medicine	1772:1792	regenerative medicine	1772:1792	These results are promising for tunable ECM-based materials for TE and regenerative medicine.
27134013	12	50	theme	encapsulated	2273:2284	arg1	cells					2286:2290	in situ encapsulated cells	2265:2290	in situ encapsulated cells	2265:2290	High hydrophilicity of HA prevents compaction of the fibrin network, while fibrin provides an adhesive environment for in situ encapsulated cells.
27134013	0	51	theme	Hyaluronic	0:9	arg1	hydrogel					55:62	Hyaluronic acid-fibrin interpenetrating double network hydrogel	0:62	Hyaluronic acid-fibrin interpenetrating double network hydrogel	0:62	Hyaluronic acid-fibrin interpenetrating double network hydrogel prepared in situ by orthogonal disulfide cross-linking reaction for biomedical applications.
27134013	5	52	theme	network	1162:1168	arg1	counterparts					1170:1181	the single network counterparts	1151:1181	the single network counterparts	1151:1181	The composition of 2% (w/v) fibrin and 1% (w/v) HA showed the highest storage modulus (G'), as compared with the single network counterparts.
27134013	3	53	with	IPNs	800:803	arg1	architectures					838:850	fibrin-resembling fibrillar architectures	810:850	fibrin-resembling fibrillar architectures	810:850	Combining thiol-derivatized HA with thrombin and 2-dithiopyridyl-modified HA with fibrinogen and then mixing the obtained liquid formulations afforded IPNs with fibrin-resembling fibrillar architectures at different ratios between fibrin and HA networks.
27134013	13	54	theme	single	2427:2432	arg1	network					2441:2447	the single fibrin network	2423:2447	the single fibrin network	2423:2447	The interpenetrating network hydrogel shows an increased stiffness along with a lower degradation rate of fibrin in comparison to the single fibrin network.
27134013	0	55	theme	interpenetrating	23:38	arg1	hydrogel					55:62	Hyaluronic acid-fibrin interpenetrating double network hydrogel	0:62	Hyaluronic acid-fibrin interpenetrating double network hydrogel	0:62	Hyaluronic acid-fibrin interpenetrating double network hydrogel prepared in situ by orthogonal disulfide cross-linking reaction for biomedical applications.
27134013	0	56	theme	network	47:53	arg1	hydrogel					55:62	Hyaluronic acid-fibrin interpenetrating double network hydrogel	0:62	Hyaluronic acid-fibrin interpenetrating double network hydrogel	0:62	Hyaluronic acid-fibrin interpenetrating double network hydrogel prepared in situ by orthogonal disulfide cross-linking reaction for biomedical applications.
27134013	5	57	theme	fibrin	1070:1075	arg1	composition					1046:1056	The composition	1042:1056	The composition of 2% (w/v) fibrin and 1% (w/v) HA	1042:1091	The composition of 2% (w/v) fibrin and 1% (w/v) HA showed the highest storage modulus (G'), as compared with the single network counterparts.
27134013	3	58	theme	thiol-derivatized	659:675	arg1	HA					677:678	thiol-derivatized HA	659:678	thiol-derivatized HA with thrombin	659:692	Combining thiol-derivatized HA with thrombin and 2-dithiopyridyl-modified HA with fibrinogen and then mixing the obtained liquid formulations afforded IPNs with fibrin-resembling fibrillar architectures at different ratios between fibrin and HA networks.
27134013	12	59	theme	network	2206:2212	arg1	compaction					2181:2190	compaction	2181:2190	compaction of the fibrin network	2181:2212	High hydrophilicity of HA prevents compaction of the fibrin network, while fibrin provides an adhesive environment for in situ encapsulated cells.
27134013	1	60	theme	orthogonal	469:478	arg1	fibrinogenesis					480:493	simultaneous and orthogonal fibrinogenesis	452:493	simultaneous and orthogonal fibrinogenesis	452:493	UNLABELLED To strengthen the mechanical properties of a fibrin gel and improve its applicability as a scaffold for tissue engineering (TE) applications, a strategy for the in situ preparation of the interpenetrating network (IPN) of fibrin and hyaluronic acid (HA) was developed on the basis of simultaneous and orthogonal fibrinogenesis and disulfide cross-linking.
27134013	6	61	from	hydrogels	1298:1306	arg1	solution					1341:1348	a fibrin-cleaving nattokinase solution	1311:1348	a fibrin-cleaving nattokinase solution	1311:1348	The degradation of fibrin in IPN hydrogels was slower than that in pure fibrin gels both during incubation of the hydrogels in a fibrin-cleaving nattokinase solution and during the culturing of cells after their encapsulation in the hydrogels.
27134013	7	62	theme	culturing	1498:1506	arg1	time					1508:1511	cell culturing time	1493:1511	cell culturing time	1493:1511	Together with the persistence of HA network, it permitted longer cell culturing time in the IPN.
27134013	5	63	theme	1	1081:1081	arg1	%					1082:1082	%	1082:1082	%	1082:1082	The composition of 2% (w/v) fibrin and 1% (w/v) HA showed the highest storage modulus (G'), as compared with the single network counterparts.
27134013	12	64	theme	HA	2169:2170	arg1	hydrophilicity					2151:2164	High hydrophilicity	2146:2164	High hydrophilicity of HA	2146:2170	High hydrophilicity of HA prevents compaction of the fibrin network, while fibrin provides an adhesive environment for in situ encapsulated cells.
27134013	8	65	theme	IPN	1626:1628	arg1	hydrogel					1630:1637	IPN hydrogel	1626:1637	IPN hydrogel	1626:1637	Moreover, the proliferation and spreading of MG63 cells that express the hyaluronan receptor CD44 in IPN hydrogel was increased, as compared with its single network analogues.
27134013	5	66	theme	%	1062:1062	arg1	fibrin					1070:1075	2% (w/v) fibrin	1061:1075	2% (w/v) fibrin	1061:1075	The composition of 2% (w/v) fibrin and 1% (w/v) HA showed the highest storage modulus (G'), as compared with the single network counterparts.
27134013	8	67	theme	cells	1575:1579	arg1	spreading					1557:1565	spreading	1557:1565	spreading	1557:1565	Moreover, the proliferation and spreading of MG63 cells that express the hyaluronan receptor CD44 in IPN hydrogel was increased, as compared with its single network analogues.
27134013	8	67	theme	cells	1575:1579	arg1	proliferation					1539:1551	proliferation	1539:1551	proliferation	1539:1551	Moreover, the proliferation and spreading of MG63 cells that express the hyaluronan receptor CD44 in IPN hydrogel was increased, as compared with its single network analogues.
27134013	10	68	theme	in	1852:1853	arg1	fabrication					1860:1870	in situ fabrication	1852:1870	in situ fabrication of injectable extracellular matrix hydrogels through simultaneous generation of networks of fibrin and hyaluronic acid (HA) that interpenetrate each other	1852:2025	STATEMENT OF SIGNIFICANCE The present work is devoted to in situ fabrication of injectable extracellular matrix hydrogels through simultaneous generation of networks of fibrin and hyaluronic acid (HA) that interpenetrate each other.
27134013	1	69	theme	cross-linking	509:521	arg1	basis					443:447	the basis	439:447	the basis of simultaneous and orthogonal fibrinogenesis and disulfide cross-linking	439:521	UNLABELLED To strengthen the mechanical properties of a fibrin gel and improve its applicability as a scaffold for tissue engineering (TE) applications, a strategy for the in situ preparation of the interpenetrating network (IPN) of fibrin and hyaluronic acid (HA) was developed on the basis of simultaneous and orthogonal fibrinogenesis and disulfide cross-linking.
27134013	10	70	theme	matrix	1900:1905	arg1	hydrogels					1907:1915	injectable extracellular matrix hydrogels	1875:1915	injectable extracellular matrix hydrogels	1875:1915	STATEMENT OF SIGNIFICANCE The present work is devoted to in situ fabrication of injectable extracellular matrix hydrogels through simultaneous generation of networks of fibrin and hyaluronic acid (HA) that interpenetrate each other.
27134013	1	71	theme	tissue	272:277	arg1	engineering					279:289	tissue engineering	272:289	tissue engineering (TE) applications	272:307	UNLABELLED To strengthen the mechanical properties of a fibrin gel and improve its applicability as a scaffold for tissue engineering (TE) applications, a strategy for the in situ preparation of the interpenetrating network (IPN) of fibrin and hyaluronic acid (HA) was developed on the basis of simultaneous and orthogonal fibrinogenesis and disulfide cross-linking.
27134013	1	71	theme	tissue	272:277	arg1	TE					292:293	TE	292:293	TE	292:293	UNLABELLED To strengthen the mechanical properties of a fibrin gel and improve its applicability as a scaffold for tissue engineering (TE) applications, a strategy for the in situ preparation of the interpenetrating network (IPN) of fibrin and hyaluronic acid (HA) was developed on the basis of simultaneous and orthogonal fibrinogenesis and disulfide cross-linking.
27134013	7	72	theme	network	1464:1470	arg1	persistence					1446:1456	the persistence	1442:1456	the persistence of HA network	1442:1470	Together with the persistence of HA network, it permitted longer cell culturing time in the IPN.
27134013	4	73	theme	networks	925:932	arg1	formation					908:916	The formation	904:916	The formation of two networks	904:932	The formation of two networks was confirmed by conducting reference experiments with the compositions lacking one of the four components.
27134013	8	74	theme	hyaluronan	1598:1607	arg1	CD44					1618:1621	the hyaluronan receptor CD44	1594:1621	the hyaluronan receptor CD44 in IPN hydrogel	1594:1637	Moreover, the proliferation and spreading of MG63 cells that express the hyaluronan receptor CD44 in IPN hydrogel was increased, as compared with its single network analogues.
27134013	10	75	theme	injectable	1875:1884	arg1	hydrogels					1907:1915	injectable extracellular matrix hydrogels	1875:1915	injectable extracellular matrix hydrogels	1875:1915	STATEMENT OF SIGNIFICANCE The present work is devoted to in situ fabrication of injectable extracellular matrix hydrogels through simultaneous generation of networks of fibrin and hyaluronic acid (HA) that interpenetrate each other.
27134013	12	76	theme	in	2265:2266	arg1	cells					2286:2290	in situ encapsulated cells	2265:2290	in situ encapsulated cells	2265:2290	High hydrophilicity of HA prevents compaction of the fibrin network, while fibrin provides an adhesive environment for in situ encapsulated cells.
27134013	2	77	theme	HA	592:593	arg1	derivatives					595:605	mutually reactive HA derivatives	574:605	mutually reactive HA derivatives bearing thiol and 2-dithiopyridyl groups	574:646	The synthetic pathway included the preparation of mutually reactive HA derivatives bearing thiol and 2-dithiopyridyl groups.
27134013	8	78	theme	network	1682:1688	arg1	analogues					1690:1698	its single network analogues	1671:1698	its single network analogues	1671:1698	Moreover, the proliferation and spreading of MG63 cells that express the hyaluronan receptor CD44 in IPN hydrogel was increased, as compared with its single network analogues.
27134013	3	79	theme	liquid	771:776	arg1	formulations					778:789	the obtained liquid formulations	758:789	the obtained liquid formulations	758:789	Combining thiol-derivatized HA with thrombin and 2-dithiopyridyl-modified HA with fibrinogen and then mixing the obtained liquid formulations afforded IPNs with fibrin-resembling fibrillar architectures at different ratios between fibrin and HA networks.
27134013	6	80	theme	pure	1251:1254	arg1	gels					1263:1266	pure fibrin gels	1251:1266	pure fibrin gels	1251:1266	The degradation of fibrin in IPN hydrogels was slower than that in pure fibrin gels both during incubation of the hydrogels in a fibrin-cleaving nattokinase solution and during the culturing of cells after their encapsulation in the hydrogels.
27134013	15	81	dep	in	2592:2593	arg1	vitro					2595:2599	vitro	2595:2599	vitro	2595:2599	This new approach can be applied for modular construction of in vitro tissue models and tissue engineering scaffolds in vivo.
27134013	1	82	theme	hyaluronic	401:410	arg1	acid					412:415	hyaluronic acid	401:415	hyaluronic acid (HA)	401:420	UNLABELLED To strengthen the mechanical properties of a fibrin gel and improve its applicability as a scaffold for tissue engineering (TE) applications, a strategy for the in situ preparation of the interpenetrating network (IPN) of fibrin and hyaluronic acid (HA) was developed on the basis of simultaneous and orthogonal fibrinogenesis and disulfide cross-linking.
27134013	1	82	theme	hyaluronic	401:410	arg1	HA					418:419	HA	418:419	HA	418:419	UNLABELLED To strengthen the mechanical properties of a fibrin gel and improve its applicability as a scaffold for tissue engineering (TE) applications, a strategy for the in situ preparation of the interpenetrating network (IPN) of fibrin and hyaluronic acid (HA) was developed on the basis of simultaneous and orthogonal fibrinogenesis and disulfide cross-linking.
27134013	3	83	with	HA	723:724	arg1	fibrinogen					731:740	fibrinogen	731:740	fibrinogen	731:740	Combining thiol-derivatized HA with thrombin and 2-dithiopyridyl-modified HA with fibrinogen and then mixing the obtained liquid formulations afforded IPNs with fibrin-resembling fibrillar architectures at different ratios between fibrin and HA networks.
27134013	3	83	with	HA	723:724	arg1	thrombin					685:692	thrombin	685:692	thrombin	685:692	Combining thiol-derivatized HA with thrombin and 2-dithiopyridyl-modified HA with fibrinogen and then mixing the obtained liquid formulations afforded IPNs with fibrin-resembling fibrillar architectures at different ratios between fibrin and HA networks.
27134013	1	84	theme	fibrin	213:218	arg1	gel					220:222	a fibrin gel	211:222	a fibrin gel	211:222	UNLABELLED To strengthen the mechanical properties of a fibrin gel and improve its applicability as a scaffold for tissue engineering (TE) applications, a strategy for the in situ preparation of the interpenetrating network (IPN) of fibrin and hyaluronic acid (HA) was developed on the basis of simultaneous and orthogonal fibrinogenesis and disulfide cross-linking.
27134013	14	85	theme	scaffold	2521:2528	arg1	remodelling					2502:2512	the remodelling	2498:2512	the remodelling of the scaffold	2498:2528	As a result, the cells have sufficient time for the remodelling of the scaffold.
27134013	12	86	theme	High	2146:2149	arg1	hydrophilicity					2151:2164	High hydrophilicity	2146:2164	High hydrophilicity of HA	2146:2170	High hydrophilicity of HA prevents compaction of the fibrin network, while fibrin provides an adhesive environment for in situ encapsulated cells.
27134013	10	87	theme	fibrin	1964:1969	arg1	networks					1952:1959	networks	1952:1959	networks of fibrin	1952:1969	STATEMENT OF SIGNIFICANCE The present work is devoted to in situ fabrication of injectable extracellular matrix hydrogels through simultaneous generation of networks of fibrin and hyaluronic acid (HA) that interpenetrate each other.
27134013	6	88	theme	nattokinase	1329:1339	arg1	solution					1341:1348	a fibrin-cleaving nattokinase solution	1311:1348	a fibrin-cleaving nattokinase solution	1311:1348	The degradation of fibrin in IPN hydrogels was slower than that in pure fibrin gels both during incubation of the hydrogels in a fibrin-cleaving nattokinase solution and during the culturing of cells after their encapsulation in the hydrogels.
27134013	1	89	theme	simultaneous	452:463	arg1	fibrinogenesis					480:493	simultaneous and orthogonal fibrinogenesis	452:493	simultaneous and orthogonal fibrinogenesis	452:493	UNLABELLED To strengthen the mechanical properties of a fibrin gel and improve its applicability as a scaffold for tissue engineering (TE) applications, a strategy for the in situ preparation of the interpenetrating network (IPN) of fibrin and hyaluronic acid (HA) was developed on the basis of simultaneous and orthogonal fibrinogenesis and disulfide cross-linking.
27134013	10	90	theme	networks	1952:1959	arg1	acid					1986:1989	hyaluronic acid	1975:1989	hyaluronic acid (HA)	1975:1994	STATEMENT OF SIGNIFICANCE The present work is devoted to in situ fabrication of injectable extracellular matrix hydrogels through simultaneous generation of networks of fibrin and hyaluronic acid (HA) that interpenetrate each other.
27134013	10	90	theme	networks	1952:1959	arg1	generation					1938:1947	simultaneous generation	1925:1947	simultaneous generation of networks of fibrin	1925:1969	STATEMENT OF SIGNIFICANCE The present work is devoted to in situ fabrication of injectable extracellular matrix hydrogels through simultaneous generation of networks of fibrin and hyaluronic acid (HA) that interpenetrate each other.
27134013	10	90	theme	networks	1952:1959	arg1	HA					1992:1993	HA	1992:1993	HA	1992:1993	STATEMENT OF SIGNIFICANCE The present work is devoted to in situ fabrication of injectable extracellular matrix hydrogels through simultaneous generation of networks of fibrin and hyaluronic acid (HA) that interpenetrate each other.
27134013	11	91	theme	cross-linking	2084:2096	arg1	combination					2052:2062	combination	2052:2062	combination of enzymatic fibrin cross-linking with orthogonal disulphide cross-linking of HA	2052:2143	This is accomplished by combination of enzymatic fibrin cross-linking with orthogonal disulphide cross-linking of HA.
27134013	2	92	theme	synthetic	528:536	arg1	pathway					538:544	The synthetic pathway	524:544	The synthetic pathway	524:544	The synthetic pathway included the preparation of mutually reactive HA derivatives bearing thiol and 2-dithiopyridyl groups.
27134013	1	93	theme	in	329:330	arg1	preparation					337:347	the in situ preparation	325:347	the in situ preparation of the interpenetrating network (IPN) of fibrin and hyaluronic acid (HA)	325:420	UNLABELLED To strengthen the mechanical properties of a fibrin gel and improve its applicability as a scaffold for tissue engineering (TE) applications, a strategy for the in situ preparation of the interpenetrating network (IPN) of fibrin and hyaluronic acid (HA) was developed on the basis of simultaneous and orthogonal fibrinogenesis and disulfide cross-linking.
27134013	6	94	theme	hydrogels	1298:1306	arg1	incubation					1280:1289	incubation	1280:1289	incubation of the hydrogels in a fibrin-cleaving nattokinase solution	1280:1348	The degradation of fibrin in IPN hydrogels was slower than that in pure fibrin gels both during incubation of the hydrogels in a fibrin-cleaving nattokinase solution and during the culturing of cells after their encapsulation in the hydrogels.
27134013	5	95	theme	highest	1104:1110	arg1	G					1129:1129	G'	1129:1130	G'	1129:1130	The composition of 2% (w/v) fibrin and 1% (w/v) HA showed the highest storage modulus (G'), as compared with the single network counterparts.
27134013	5	95	theme	highest	1104:1110	arg1	modulus					1120:1126	the highest storage modulus	1100:1126	the highest storage modulus (G')	1100:1131	The composition of 2% (w/v) fibrin and 1% (w/v) HA showed the highest storage modulus (G'), as compared with the single network counterparts.
27134013	6	96	theme	IPN	1213:1215	arg1	hydrogels					1217:1225	IPN hydrogels	1213:1225	IPN hydrogels	1213:1225	The degradation of fibrin in IPN hydrogels was slower than that in pure fibrin gels both during incubation of the hydrogels in a fibrin-cleaving nattokinase solution and during the culturing of cells after their encapsulation in the hydrogels.
27134013	6	97	from	hydrogels	1217:1225	arg1	degradation					1188:1198	The degradation	1184:1198	The degradation of fibrin in IPN hydrogels	1184:1225	The degradation of fibrin in IPN hydrogels was slower than that in pure fibrin gels both during incubation of the hydrogels in a fibrin-cleaving nattokinase solution and during the culturing of cells after their encapsulation in the hydrogels.
27134013	6	97	from	hydrogels	1217:1225	arg1	slower					1231:1236	slower	1231:1236	slower	1231:1236	The degradation of fibrin in IPN hydrogels was slower than that in pure fibrin gels both during incubation of the hydrogels in a fibrin-cleaving nattokinase solution and during the culturing of cells after their encapsulation in the hydrogels.
27134013	3	98	theme	fibrillar	828:836	arg1	architectures					838:850	fibrin-resembling fibrillar architectures	810:850	fibrin-resembling fibrillar architectures	810:850	Combining thiol-derivatized HA with thrombin and 2-dithiopyridyl-modified HA with fibrinogen and then mixing the obtained liquid formulations afforded IPNs with fibrin-resembling fibrillar architectures at different ratios between fibrin and HA networks.
27134013	1	99	theme	acid	412:415	arg1	network					373:379	the interpenetrating network	352:379	the interpenetrating network (IPN) of fibrin and hyaluronic acid (HA)	352:420	UNLABELLED To strengthen the mechanical properties of a fibrin gel and improve its applicability as a scaffold for tissue engineering (TE) applications, a strategy for the in situ preparation of the interpenetrating network (IPN) of fibrin and hyaluronic acid (HA) was developed on the basis of simultaneous and orthogonal fibrinogenesis and disulfide cross-linking.
27134013	1	99	theme	acid	412:415	arg1	IPN					382:384	IPN	382:384	IPN	382:384	UNLABELLED To strengthen the mechanical properties of a fibrin gel and improve its applicability as a scaffold for tissue engineering (TE) applications, a strategy for the in situ preparation of the interpenetrating network (IPN) of fibrin and hyaluronic acid (HA) was developed on the basis of simultaneous and orthogonal fibrinogenesis and disulfide cross-linking.
27134013	10	100	theme	present	1825:1831	arg1	devoted					1841:1847	devoted	1841:1847	devoted	1841:1847	STATEMENT OF SIGNIFICANCE The present work is devoted to in situ fabrication of injectable extracellular matrix hydrogels through simultaneous generation of networks of fibrin and hyaluronic acid (HA) that interpenetrate each other.
27134013	10	100	theme	present	1825:1831	arg1	work					1833:1836	The present work	1821:1836	The present work	1821:1836	STATEMENT OF SIGNIFICANCE The present work is devoted to in situ fabrication of injectable extracellular matrix hydrogels through simultaneous generation of networks of fibrin and hyaluronic acid (HA) that interpenetrate each other.
27134013	13	101	theme	interpenetrating	2297:2312	arg1	hydrogel					2322:2329	The interpenetrating network hydrogel	2293:2329	The interpenetrating network hydrogel	2293:2329	The interpenetrating network hydrogel shows an increased stiffness along with a lower degradation rate of fibrin in comparison to the single fibrin network.
27134013	5	102	dep	%	1062:1062	arg1	w/v					1065:1067	w/v	1065:1067	w/v	1065:1067	The composition of 2% (w/v) fibrin and 1% (w/v) HA showed the highest storage modulus (G'), as compared with the single network counterparts.
27134013	11	103	theme	fibrin	2077:2082	arg1	cross-linking					2084:2096	enzymatic fibrin cross-linking	2067:2096	enzymatic fibrin cross-linking	2067:2096	This is accomplished by combination of enzymatic fibrin cross-linking with orthogonal disulphide cross-linking of HA.
27134013	8	104	dep	proliferation	1539:1551	arg1	the					1535:1537	the	1535:1537	the	1535:1537	Moreover, the proliferation and spreading of MG63 cells that express the hyaluronan receptor CD44 in IPN hydrogel was increased, as compared with its single network analogues.
27134013	6	105	theme	fibrin	1203:1208	arg1	degradation					1188:1198	The degradation	1184:1198	The degradation of fibrin in IPN hydrogels	1184:1225	The degradation of fibrin in IPN hydrogels was slower than that in pure fibrin gels both during incubation of the hydrogels in a fibrin-cleaving nattokinase solution and during the culturing of cells after their encapsulation in the hydrogels.
27134013	6	105	theme	fibrin	1203:1208	arg1	slower					1231:1236	slower	1231:1236	slower	1231:1236	The degradation of fibrin in IPN hydrogels was slower than that in pure fibrin gels both during incubation of the hydrogels in a fibrin-cleaving nattokinase solution and during the culturing of cells after their encapsulation in the hydrogels.
27134013	3	106	theme	fibrin	880:885	arg1	networks					894:901	fibrin and HA networks	880:901	fibrin and HA networks	880:901	Combining thiol-derivatized HA with thrombin and 2-dithiopyridyl-modified HA with fibrinogen and then mixing the obtained liquid formulations afforded IPNs with fibrin-resembling fibrillar architectures at different ratios between fibrin and HA networks.
27134013	10	107	theme	SIGNIFICANCE	1808:1819	arg1	STATEMENT					1795:1803	STATEMENT	1795:1803	STATEMENT	1795:1803	STATEMENT OF SIGNIFICANCE The present work is devoted to in situ fabrication of injectable extracellular matrix hydrogels through simultaneous generation of networks of fibrin and hyaluronic acid (HA) that interpenetrate each other.
27134013	15	108	theme	in	2592:2593	arg1	models					2608:2613	in vitro tissue models	2592:2613	in vitro tissue models	2592:2613	This new approach can be applied for modular construction of in vitro tissue models and tissue engineering scaffolds in vivo.
27134013	0	109	theme	disulfide	95:103	arg1	reaction					119:126	orthogonal disulfide cross-linking reaction	84:126	orthogonal disulfide cross-linking reaction for biomedical applications	84:154	Hyaluronic acid-fibrin interpenetrating double network hydrogel prepared in situ by orthogonal disulfide cross-linking reaction for biomedical applications.
27134013	1	110	theme	mechanical	186:195	arg1	properties					197:206	the mechanical properties	182:206	the mechanical properties of a fibrin gel	182:222	UNLABELLED To strengthen the mechanical properties of a fibrin gel and improve its applicability as a scaffold for tissue engineering (TE) applications, a strategy for the in situ preparation of the interpenetrating network (IPN) of fibrin and hyaluronic acid (HA) was developed on the basis of simultaneous and orthogonal fibrinogenesis and disulfide cross-linking.
27134013	1	111	theme	interpenetrating	356:371	arg1	network					373:379	the interpenetrating network	352:379	the interpenetrating network (IPN) of fibrin and hyaluronic acid (HA)	352:420	UNLABELLED To strengthen the mechanical properties of a fibrin gel and improve its applicability as a scaffold for tissue engineering (TE) applications, a strategy for the in situ preparation of the interpenetrating network (IPN) of fibrin and hyaluronic acid (HA) was developed on the basis of simultaneous and orthogonal fibrinogenesis and disulfide cross-linking.
27134013	1	111	theme	interpenetrating	356:371	arg1	IPN					382:384	IPN	382:384	IPN	382:384	UNLABELLED To strengthen the mechanical properties of a fibrin gel and improve its applicability as a scaffold for tissue engineering (TE) applications, a strategy for the in situ preparation of the interpenetrating network (IPN) of fibrin and hyaluronic acid (HA) was developed on the basis of simultaneous and orthogonal fibrinogenesis and disulfide cross-linking.
27134013	9	112	theme	ECM-based	1741:1749	arg1	materials					1751:1759	tunable ECM-based materials	1733:1759	tunable ECM-based materials for TE and regenerative medicine	1733:1792	These results are promising for tunable ECM-based materials for TE and regenerative medicine.
27134013	13	113	theme	degradation	2379:2389	arg1	rate					2391:2394	a lower degradation rate	2371:2394	a lower degradation rate of fibrin in comparison to the single fibrin network	2371:2447	The interpenetrating network hydrogel shows an increased stiffness along with a lower degradation rate of fibrin in comparison to the single fibrin network.
27134013	13	114	theme	increased	2340:2348	arg1	stiffness					2350:2358	an increased stiffness	2337:2358	an increased stiffness along with a lower degradation rate of fibrin in comparison to the single fibrin network	2337:2447	The interpenetrating network hydrogel shows an increased stiffness along with a lower degradation rate of fibrin in comparison to the single fibrin network.
27134013	13	115	from	rate	2391:2394	arg1	comparison					2409:2418	comparison	2409:2418	comparison to the single fibrin network	2409:2447	The interpenetrating network hydrogel shows an increased stiffness along with a lower degradation rate of fibrin in comparison to the single fibrin network.
27134013	3	116	theme	HA	891:892	arg1	networks					894:901	fibrin and HA networks	880:901	fibrin and HA networks	880:901	Combining thiol-derivatized HA with thrombin and 2-dithiopyridyl-modified HA with fibrinogen and then mixing the obtained liquid formulations afforded IPNs with fibrin-resembling fibrillar architectures at different ratios between fibrin and HA networks.
27134013	0	117	theme	biomedical	132:141	arg1	applications					143:154	biomedical applications	132:154	biomedical applications	132:154	Hyaluronic acid-fibrin interpenetrating double network hydrogel prepared in situ by orthogonal disulfide cross-linking reaction for biomedical applications.
27134013	6	118	from	solution	1341:1348	arg1	incubation					1280:1289	incubation	1280:1289	incubation of the hydrogels in a fibrin-cleaving nattokinase solution	1280:1348	The degradation of fibrin in IPN hydrogels was slower than that in pure fibrin gels both during incubation of the hydrogels in a fibrin-cleaving nattokinase solution and during the culturing of cells after their encapsulation in the hydrogels.
27134013	10	119	dep	in	1852:1853	arg1	situ					1855:1858	situ	1855:1858	situ	1855:1858	STATEMENT OF SIGNIFICANCE The present work is devoted to in situ fabrication of injectable extracellular matrix hydrogels through simultaneous generation of networks of fibrin and hyaluronic acid (HA) that interpenetrate each other.
27134013	0	120	theme	acid-fibrin	11:21	arg1	hydrogel					55:62	Hyaluronic acid-fibrin interpenetrating double network hydrogel	0:62	Hyaluronic acid-fibrin interpenetrating double network hydrogel	0:62	Hyaluronic acid-fibrin interpenetrating double network hydrogel prepared in situ by orthogonal disulfide cross-linking reaction for biomedical applications.
27134013	1	121	theme	network	373:379	arg1	preparation					337:347	the in situ preparation	325:347	the in situ preparation of the interpenetrating network (IPN) of fibrin and hyaluronic acid (HA)	325:420	UNLABELLED To strengthen the mechanical properties of a fibrin gel and improve its applicability as a scaffold for tissue engineering (TE) applications, a strategy for the in situ preparation of the interpenetrating network (IPN) of fibrin and hyaluronic acid (HA) was developed on the basis of simultaneous and orthogonal fibrinogenesis and disulfide cross-linking.
27134013	13	122	from	comparison	2409:2418	arg1	rate					2391:2394	a lower degradation rate	2371:2394	a lower degradation rate of fibrin in comparison to the single fibrin network	2371:2447	The interpenetrating network hydrogel shows an increased stiffness along with a lower degradation rate of fibrin in comparison to the single fibrin network.
27134013	0	123	theme	double	40:45	arg1	hydrogel					55:62	Hyaluronic acid-fibrin interpenetrating double network hydrogel	0:62	Hyaluronic acid-fibrin interpenetrating double network hydrogel	0:62	Hyaluronic acid-fibrin interpenetrating double network hydrogel prepared in situ by orthogonal disulfide cross-linking reaction for biomedical applications.
27134013	13	124	theme	fibrin	2434:2439	arg1	network					2441:2447	the single fibrin network	2423:2447	the single fibrin network	2423:2447	The interpenetrating network hydrogel shows an increased stiffness along with a lower degradation rate of fibrin in comparison to the single fibrin network.
27134013	14	125	contain	have	2473:2476	arg1	cells					2467:2471	the cells	2463:2471	the cells	2463:2471	As a result, the cells have sufficient time for the remodelling of the scaffold.
27134013	14	125	contain	have	2473:2476	arg2	result					2455:2460	a result	2453:2460	a result	2453:2460	As a result, the cells have sufficient time for the remodelling of the scaffold.
27134013	14	125	contain	have	2473:2476	arg2	time					2489:2492	sufficient time	2478:2492	sufficient time	2478:2492	As a result, the cells have sufficient time for the remodelling of the scaffold.
27134013	13	126	from	fibrin	2399:2404	arg1	comparison					2409:2418	comparison	2409:2418	comparison to the single fibrin network	2409:2447	The interpenetrating network hydrogel shows an increased stiffness along with a lower degradation rate of fibrin in comparison to the single fibrin network.
27134013	12	127	dep	in	2265:2266	arg1	situ					2268:2271	situ	2268:2271	situ	2268:2271	High hydrophilicity of HA prevents compaction of the fibrin network, while fibrin provides an adhesive environment for in situ encapsulated cells.
27134013	13	128	theme	fibrin	2399:2404	arg1	rate					2391:2394	a lower degradation rate	2371:2394	a lower degradation rate of fibrin in comparison to the single fibrin network	2371:2447	The interpenetrating network hydrogel shows an increased stiffness along with a lower degradation rate of fibrin in comparison to the single fibrin network.
27134013	11	129	with	combination	2052:2062	arg1	cross-linking					2125:2137	orthogonal disulphide cross-linking	2103:2137	orthogonal disulphide cross-linking of HA	2103:2143	This is accomplished by combination of enzymatic fibrin cross-linking with orthogonal disulphide cross-linking of HA.
27134013	6	130	from	incubation	1280:1289	arg1	solution					1341:1348	a fibrin-cleaving nattokinase solution	1311:1348	a fibrin-cleaving nattokinase solution	1311:1348	The degradation of fibrin in IPN hydrogels was slower than that in pure fibrin gels both during incubation of the hydrogels in a fibrin-cleaving nattokinase solution and during the culturing of cells after their encapsulation in the hydrogels.
27134013	8	131	theme	MG63	1570:1573	arg1	cells					1575:1579	MG63 cells	1570:1579	MG63 cells that express the hyaluronan receptor CD44 in IPN hydrogel	1570:1637	Moreover, the proliferation and spreading of MG63 cells that express the hyaluronan receptor CD44 in IPN hydrogel was increased, as compared with its single network analogues.
27134013	3	132	theme	2-dithiopyridyl-modified	698:721	arg1	HA					723:724	2-dithiopyridyl-modified HA	698:724	2-dithiopyridyl-modified HA with fibrinogen	698:740	Combining thiol-derivatized HA with thrombin and 2-dithiopyridyl-modified HA with fibrinogen and then mixing the obtained liquid formulations afforded IPNs with fibrin-resembling fibrillar architectures at different ratios between fibrin and HA networks.
27134013	12	133	theme	fibrin	2199:2204	arg1	network					2206:2212	the fibrin network	2195:2212	the fibrin network	2195:2212	High hydrophilicity of HA prevents compaction of the fibrin network, while fibrin provides an adhesive environment for in situ encapsulated cells.
27134013	1	134	theme	fibrinogenesis	480:493	arg1	basis					443:447	the basis	439:447	the basis of simultaneous and orthogonal fibrinogenesis and disulfide cross-linking	439:521	UNLABELLED To strengthen the mechanical properties of a fibrin gel and improve its applicability as a scaffold for tissue engineering (TE) applications, a strategy for the in situ preparation of the interpenetrating network (IPN) of fibrin and hyaluronic acid (HA) was developed on the basis of simultaneous and orthogonal fibrinogenesis and disulfide cross-linking.
27134013	5	135	theme	single	1155:1160	arg1	counterparts					1170:1181	the single network counterparts	1151:1181	the single network counterparts	1151:1181	The composition of 2% (w/v) fibrin and 1% (w/v) HA showed the highest storage modulus (G'), as compared with the single network counterparts.
27134013	5	136	theme	2	1061:1061	arg1	%					1062:1062	%	1062:1062	%	1062:1062	The composition of 2% (w/v) fibrin and 1% (w/v) HA showed the highest storage modulus (G'), as compared with the single network counterparts.
27134013	1	137	theme	disulfide	499:507	arg1	cross-linking					509:521	disulfide cross-linking	499:521	disulfide cross-linking	499:521	UNLABELLED To strengthen the mechanical properties of a fibrin gel and improve its applicability as a scaffold for tissue engineering (TE) applications, a strategy for the in situ preparation of the interpenetrating network (IPN) of fibrin and hyaluronic acid (HA) was developed on the basis of simultaneous and orthogonal fibrinogenesis and disulfide cross-linking.
27134013	15	138	theme	modular	2568:2574	arg1	construction					2576:2587	modular construction	2568:2587	modular construction of in vitro tissue models and tissue engineering scaffolds	2568:2646	This new approach can be applied for modular construction of in vitro tissue models and tissue engineering scaffolds in vivo.
27134013	7	139	theme	cell	1493:1496	arg1	time					1508:1511	cell culturing time	1493:1511	cell culturing time	1493:1511	Together with the persistence of HA network, it permitted longer cell culturing time in the IPN.
27134013	10	140	theme	hydrogels	1907:1915	arg1	fabrication					1860:1870	in situ fabrication	1852:1870	in situ fabrication of injectable extracellular matrix hydrogels through simultaneous generation of networks of fibrin and hyaluronic acid (HA) that interpenetrate each other	1852:2025	STATEMENT OF SIGNIFICANCE The present work is devoted to in situ fabrication of injectable extracellular matrix hydrogels through simultaneous generation of networks of fibrin and hyaluronic acid (HA) that interpenetrate each other.
26686158	4	0	theme	fibres	892:897	arg1	adhesion					865:872	improved adhesion	856:872	improved adhesion	856:872	PLA/EVA-GMA/CF ternary composites displayed improved adhesion and dispersion of fibres into the matrix as compared to PLA/CF system.
26686158	4	0	theme	fibres	892:897	arg1	dispersion					878:887	dispersion	878:887	dispersion	878:887	PLA/EVA-GMA/CF ternary composites displayed improved adhesion and dispersion of fibres into the matrix as compared to PLA/CF system.
26686158	3	1	theme	PLA	635:637	arg1	rate					657:660	PLA spherulite growth rate	635:660	PLA spherulite growth rate	635:660	The miscibility and morphology of PLA/EVA-GMA blends were first examined as a function of composition: a large rise of PLA spherulite growth rate in the blends was discovered with increasing the EVA-GMA content (0-30 wt%) in the isothermal crystallization both from the melt and the solid state.
26686158	4	2	theme	PLA/CF	930:935	arg1	system					937:942	PLA/CF system	930:942	PLA/CF system	930:942	PLA/EVA-GMA/CF ternary composites displayed improved adhesion and dispersion of fibres into the matrix as compared to PLA/CF system.
26686158	1	3	theme	cellulose	310:318	arg1	CF					334:335	CF	334:335	CF	334:335	The effect of addition of an ethylene-vinyl acetate copolymer modified with glycidyl methacrylate (EVA-GMA) on the structure and properties of poly(lactic acid) (PLA) composites with cellulose micro fibres (CF) was investigated.
26686158	1	3	theme	cellulose	310:318	arg1	fibres					326:331	cellulose micro fibres	310:331	cellulose micro fibres (CF)	310:336	The effect of addition of an ethylene-vinyl acetate copolymer modified with glycidyl methacrylate (EVA-GMA) on the structure and properties of poly(lactic acid) (PLA) composites with cellulose micro fibres (CF) was investigated.
26686158	5	4	from	break	1048:1052	arg1	modulus					1012:1018	elastic modulus	1004:1018	elastic modulus	1004:1018	Marked changes of thermodynamic and tensile parameters, as elastic modulus, strength and elongation at break were observed for the composites, depending on blend composition, polymer miscibility and fibre-matrix chemical interactions at the interface.
26686158	5	4	from	break	1048:1052	arg1	elongation					1034:1043	elongation	1034:1043	elongation	1034:1043	Marked changes of thermodynamic and tensile parameters, as elastic modulus, strength and elongation at break were observed for the composites, depending on blend composition, polymer miscibility and fibre-matrix chemical interactions at the interface.
26686158	5	4	from	break	1048:1052	arg1	changes					952:958	Marked changes	945:958	Marked changes of thermodynamic and tensile parameters	945:998	Marked changes of thermodynamic and tensile parameters, as elastic modulus, strength and elongation at break were observed for the composites, depending on blend composition, polymer miscibility and fibre-matrix chemical interactions at the interface.
26686158	5	4	from	break	1048:1052	arg1	strength					1021:1028	strength	1021:1028	strength	1021:1028	Marked changes of thermodynamic and tensile parameters, as elastic modulus, strength and elongation at break were observed for the composites, depending on blend composition, polymer miscibility and fibre-matrix chemical interactions at the interface.
26686158	3	5	theme	PLA/EVA-GMA	550:560	arg1	blends					562:567	PLA/EVA-GMA blends	550:567	PLA/EVA-GMA blends	550:567	The miscibility and morphology of PLA/EVA-GMA blends were first examined as a function of composition: a large rise of PLA spherulite growth rate in the blends was discovered with increasing the EVA-GMA content (0-30 wt%) in the isothermal crystallization both from the melt and the solid state.
26686158	1	6	with	composites	294:303	arg1	CF					334:335	CF	334:335	CF	334:335	The effect of addition of an ethylene-vinyl acetate copolymer modified with glycidyl methacrylate (EVA-GMA) on the structure and properties of poly(lactic acid) (PLA) composites with cellulose micro fibres (CF) was investigated.
26686158	1	6	with	composites	294:303	arg1	fibres					326:331	cellulose micro fibres	310:331	cellulose micro fibres (CF)	310:336	The effect of addition of an ethylene-vinyl acetate copolymer modified with glycidyl methacrylate (EVA-GMA) on the structure and properties of poly(lactic acid) (PLA) composites with cellulose micro fibres (CF) was investigated.
26686158	3	7	theme	0-30	728:731	arg1	content					719:725	the EVA-GMA content	707:725	the EVA-GMA content (0-30 wt%)	707:736	The miscibility and morphology of PLA/EVA-GMA blends were first examined as a function of composition: a large rise of PLA spherulite growth rate in the blends was discovered with increasing the EVA-GMA content (0-30 wt%) in the isothermal crystallization both from the melt and the solid state.
26686158	3	7	theme	0-30	728:731	arg1	%					735:735	0-30 wt%	728:735	0-30 wt%	728:735	The miscibility and morphology of PLA/EVA-GMA blends were first examined as a function of composition: a large rise of PLA spherulite growth rate in the blends was discovered with increasing the EVA-GMA content (0-30 wt%) in the isothermal crystallization both from the melt and the solid state.
26686158	0	8	theme	modified	84:91	arg1	copolymer					116:124	modified ethylene-vinyl acetate copolymer	84:124	modified ethylene-vinyl acetate copolymer	84:124	Property tuning of poly(lactic acid)/cellulose bio-composites through blending with modified ethylene-vinyl acetate copolymer.
26686158	1	9	theme	micro	320:324	arg1	CF					334:335	CF	334:335	CF	334:335	The effect of addition of an ethylene-vinyl acetate copolymer modified with glycidyl methacrylate (EVA-GMA) on the structure and properties of poly(lactic acid) (PLA) composites with cellulose micro fibres (CF) was investigated.
26686158	1	9	theme	micro	320:324	arg1	fibres					326:331	cellulose micro fibres	310:331	cellulose micro fibres (CF)	310:336	The effect of addition of an ethylene-vinyl acetate copolymer modified with glycidyl methacrylate (EVA-GMA) on the structure and properties of poly(lactic acid) (PLA) composites with cellulose micro fibres (CF) was investigated.
26686158	2	10	theme	tensile	501:507	arg1	tests					509:513	tensile tests	501:513	tensile tests	501:513	Binary (PLA/CF) and ternary (PLA/EVA-GMA/CF) composites obtained by melt mixing in Brabender mixer were analysed by SEM, POM, WAXS, DSC, TGA and tensile tests.
26686158	3	11	theme	isothermal	745:754	arg1	crystallization					756:770	the isothermal crystallization	741:770	the isothermal crystallization	741:770	The miscibility and morphology of PLA/EVA-GMA blends were first examined as a function of composition: a large rise of PLA spherulite growth rate in the blends was discovered with increasing the EVA-GMA content (0-30 wt%) in the isothermal crystallization both from the melt and the solid state.
26686158	3	12	theme	EVA-GMA	711:717	arg1	content					719:725	the EVA-GMA content	707:725	the EVA-GMA content (0-30 wt%)	707:736	The miscibility and morphology of PLA/EVA-GMA blends were first examined as a function of composition: a large rise of PLA spherulite growth rate in the blends was discovered with increasing the EVA-GMA content (0-30 wt%) in the isothermal crystallization both from the melt and the solid state.
26686158	3	12	theme	EVA-GMA	711:717	arg1	%					735:735	0-30 wt%	728:735	0-30 wt%	728:735	The miscibility and morphology of PLA/EVA-GMA blends were first examined as a function of composition: a large rise of PLA spherulite growth rate in the blends was discovered with increasing the EVA-GMA content (0-30 wt%) in the isothermal crystallization both from the melt and the solid state.
26686158	4	13	theme	PLA/EVA-GMA/CF	812:825	arg1	composites					835:844	PLA/EVA-GMA/CF ternary composites	812:844	PLA/EVA-GMA/CF ternary composites	812:844	PLA/EVA-GMA/CF ternary composites displayed improved adhesion and dispersion of fibres into the matrix as compared to PLA/CF system.
26686158	5	14	theme	tensile	981:987	arg1	parameters					989:998	thermodynamic and tensile parameters	963:998	parameters	989:998	Marked changes of thermodynamic and tensile parameters, as elastic modulus, strength and elongation at break were observed for the composites, depending on blend composition, polymer miscibility and fibre-matrix chemical interactions at the interface.
26686158	5	15	theme	elastic	1004:1010	arg1	modulus					1012:1018	elastic modulus	1004:1018	elastic modulus	1004:1018	Marked changes of thermodynamic and tensile parameters, as elastic modulus, strength and elongation at break were observed for the composites, depending on blend composition, polymer miscibility and fibre-matrix chemical interactions at the interface.
26686158	5	15	theme	elastic	1004:1010	arg1	elongation					1034:1043	elongation	1034:1043	elongation	1034:1043	Marked changes of thermodynamic and tensile parameters, as elastic modulus, strength and elongation at break were observed for the composites, depending on blend composition, polymer miscibility and fibre-matrix chemical interactions at the interface.
26686158	5	15	theme	elastic	1004:1010	arg1	changes					952:958	Marked changes	945:958	Marked changes of thermodynamic and tensile parameters	945:998	Marked changes of thermodynamic and tensile parameters, as elastic modulus, strength and elongation at break were observed for the composites, depending on blend composition, polymer miscibility and fibre-matrix chemical interactions at the interface.
26686158	5	15	theme	elastic	1004:1010	arg1	strength					1021:1028	strength	1021:1028	strength	1021:1028	Marked changes of thermodynamic and tensile parameters, as elastic modulus, strength and elongation at break were observed for the composites, depending on blend composition, polymer miscibility and fibre-matrix chemical interactions at the interface.
26686158	0	16	theme	acetate	108:114	arg1	copolymer					116:124	modified ethylene-vinyl acetate copolymer	84:124	modified ethylene-vinyl acetate copolymer	84:124	Property tuning of poly(lactic acid)/cellulose bio-composites through blending with modified ethylene-vinyl acetate copolymer.
26686158	5	17	theme	parameters	989:998	arg1	modulus					1012:1018	elastic modulus	1004:1018	elastic modulus	1004:1018	Marked changes of thermodynamic and tensile parameters, as elastic modulus, strength and elongation at break were observed for the composites, depending on blend composition, polymer miscibility and fibre-matrix chemical interactions at the interface.
26686158	5	17	theme	parameters	989:998	arg1	elongation					1034:1043	elongation	1034:1043	elongation	1034:1043	Marked changes of thermodynamic and tensile parameters, as elastic modulus, strength and elongation at break were observed for the composites, depending on blend composition, polymer miscibility and fibre-matrix chemical interactions at the interface.
26686158	5	17	theme	parameters	989:998	arg1	changes					952:958	Marked changes	945:958	Marked changes of thermodynamic and tensile parameters	945:998	Marked changes of thermodynamic and tensile parameters, as elastic modulus, strength and elongation at break were observed for the composites, depending on blend composition, polymer miscibility and fibre-matrix chemical interactions at the interface.
26686158	5	17	theme	parameters	989:998	arg1	strength					1021:1028	strength	1021:1028	strength	1021:1028	Marked changes of thermodynamic and tensile parameters, as elastic modulus, strength and elongation at break were observed for the composites, depending on blend composition, polymer miscibility and fibre-matrix chemical interactions at the interface.
26686158	3	18	dep	miscibility	520:530	arg1	The					516:518	The	516:518	The	516:518	The miscibility and morphology of PLA/EVA-GMA blends were first examined as a function of composition: a large rise of PLA spherulite growth rate in the blends was discovered with increasing the EVA-GMA content (0-30 wt%) in the isothermal crystallization both from the melt and the solid state.
26686158	1	19	theme	ethylene-vinyl	156:169	arg1	copolymer					179:187	an ethylene-vinyl acetate copolymer	153:187	an ethylene-vinyl acetate copolymer modified with glycidyl methacrylate (EVA-GMA)	153:233	The effect of addition of an ethylene-vinyl acetate copolymer modified with glycidyl methacrylate (EVA-GMA) on the structure and properties of poly(lactic acid) (PLA) composites with cellulose micro fibres (CF) was investigated.
26686158	0	20	theme	ethylene-vinyl	93:106	arg1	copolymer					116:124	modified ethylene-vinyl acetate copolymer	84:124	modified ethylene-vinyl acetate copolymer	84:124	Property tuning of poly(lactic acid)/cellulose bio-composites through blending with modified ethylene-vinyl acetate copolymer.
26686158	2	21	theme	melt	424:427	arg1	mixing					429:434	melt mixing	424:434	melt mixing in Brabender mixer	424:453	Binary (PLA/CF) and ternary (PLA/EVA-GMA/CF) composites obtained by melt mixing in Brabender mixer were analysed by SEM, POM, WAXS, DSC, TGA and tensile tests.
26686158	1	22	theme	acetate	171:177	arg1	copolymer					179:187	an ethylene-vinyl acetate copolymer	153:187	an ethylene-vinyl acetate copolymer modified with glycidyl methacrylate (EVA-GMA)	153:233	The effect of addition of an ethylene-vinyl acetate copolymer modified with glycidyl methacrylate (EVA-GMA) on the structure and properties of poly(lactic acid) (PLA) composites with cellulose micro fibres (CF) was investigated.
26686158	5	23	theme	fibre-matrix	1144:1155	arg1	interactions					1166:1177	fibre-matrix chemical interactions	1144:1177	fibre-matrix chemical interactions at the interface	1144:1194	Marked changes of thermodynamic and tensile parameters, as elastic modulus, strength and elongation at break were observed for the composites, depending on blend composition, polymer miscibility and fibre-matrix chemical interactions at the interface.
26686158	3	24	theme	large	621:625	arg1	rise					627:630	a large rise	619:630	a large rise of PLA spherulite growth rate in the blends	619:674	The miscibility and morphology of PLA/EVA-GMA blends were first examined as a function of composition: a large rise of PLA spherulite growth rate in the blends was discovered with increasing the EVA-GMA content (0-30 wt%) in the isothermal crystallization both from the melt and the solid state.
26686158	1	25	from	effect	131:136	arg1	properties					256:265	properties	256:265	properties	256:265	The effect of addition of an ethylene-vinyl acetate copolymer modified with glycidyl methacrylate (EVA-GMA) on the structure and properties of poly(lactic acid) (PLA) composites with cellulose micro fibres (CF) was investigated.
26686158	1	25	from	effect	131:136	arg1	structure					242:250	the structure	238:250	the structure	238:250	The effect of addition of an ethylene-vinyl acetate copolymer modified with glycidyl methacrylate (EVA-GMA) on the structure and properties of poly(lactic acid) (PLA) composites with cellulose micro fibres (CF) was investigated.
26686158	1	26	theme	copolymer	179:187	arg1	addition					141:148	addition	141:148	addition of an ethylene-vinyl acetate copolymer modified with glycidyl methacrylate (EVA-GMA)	141:233	The effect of addition of an ethylene-vinyl acetate copolymer modified with glycidyl methacrylate (EVA-GMA) on the structure and properties of poly(lactic acid) (PLA) composites with cellulose micro fibres (CF) was investigated.
26686158	0	27	theme	Property	0:7	arg1	tuning					9:14	Property tuning	0:14	Property tuning of poly(lactic acid)/cellulose	0:45	Property tuning of poly(lactic acid)/cellulose bio-composites through blending with modified ethylene-vinyl acetate copolymer.
26686158	1	28	theme	poly	270:273	arg1	composites					294:303	poly(lactic acid) (PLA) composites	270:303	poly(lactic acid) (PLA) composites with cellulose micro fibres (CF)	270:336	The effect of addition of an ethylene-vinyl acetate copolymer modified with glycidyl methacrylate (EVA-GMA) on the structure and properties of poly(lactic acid) (PLA) composites with cellulose micro fibres (CF) was investigated.
26686158	3	29	theme	growth	650:655	arg1	rate					657:660	PLA spherulite growth rate	635:660	PLA spherulite growth rate	635:660	The miscibility and morphology of PLA/EVA-GMA blends were first examined as a function of composition: a large rise of PLA spherulite growth rate in the blends was discovered with increasing the EVA-GMA content (0-30 wt%) in the isothermal crystallization both from the melt and the solid state.
26686158	3	30	dep	crystallization	756:770	arg1	both					772:775	both	772:775	both	772:775	The miscibility and morphology of PLA/EVA-GMA blends were first examined as a function of composition: a large rise of PLA spherulite growth rate in the blends was discovered with increasing the EVA-GMA content (0-30 wt%) in the isothermal crystallization both from the melt and the solid state.
26686158	5	31	from	interface	1186:1194	arg1	composition					1107:1117	blend composition	1101:1117	blend composition	1101:1117	Marked changes of thermodynamic and tensile parameters, as elastic modulus, strength and elongation at break were observed for the composites, depending on blend composition, polymer miscibility and fibre-matrix chemical interactions at the interface.
26686158	5	31	from	interface	1186:1194	arg1	miscibility					1128:1138	polymer miscibility	1120:1138	polymer miscibility	1120:1138	Marked changes of thermodynamic and tensile parameters, as elastic modulus, strength and elongation at break were observed for the composites, depending on blend composition, polymer miscibility and fibre-matrix chemical interactions at the interface.
26686158	5	31	from	interface	1186:1194	arg1	interactions					1166:1177	fibre-matrix chemical interactions	1144:1177	fibre-matrix chemical interactions at the interface	1144:1194	Marked changes of thermodynamic and tensile parameters, as elastic modulus, strength and elongation at break were observed for the composites, depending on blend composition, polymer miscibility and fibre-matrix chemical interactions at the interface.
26686158	0	32	theme	poly	19:22	arg1	/cellulose					36:45	poly(lactic acid)/cellulose	19:45	poly(lactic acid)/cellulose	19:45	Property tuning of poly(lactic acid)/cellulose bio-composites through blending with modified ethylene-vinyl acetate copolymer.
26686158	3	33	theme	rate	657:660	arg1	rise					627:630	a large rise	619:630	a large rise of PLA spherulite growth rate in the blends	619:674	The miscibility and morphology of PLA/EVA-GMA blends were first examined as a function of composition: a large rise of PLA spherulite growth rate in the blends was discovered with increasing the EVA-GMA content (0-30 wt%) in the isothermal crystallization both from the melt and the solid state.
26686158	2	34	theme	ternary	376:382	arg1	composites					401:410	Binary (PLA/CF) and ternary (PLA/EVA-GMA/CF) composites	356:410	Binary (PLA/CF) and ternary (PLA/EVA-GMA/CF) composites obtained by melt mixing in Brabender mixer	356:453	Binary (PLA/CF) and ternary (PLA/EVA-GMA/CF) composites obtained by melt mixing in Brabender mixer were analysed by SEM, POM, WAXS, DSC, TGA and tensile tests.
26686158	1	35	theme	lactic	275:280	arg1	poly					270:273	poly	270:273	poly(lactic acid) (PLA) composites with cellulose micro fibres (CF)	270:336	The effect of addition of an ethylene-vinyl acetate copolymer modified with glycidyl methacrylate (EVA-GMA) on the structure and properties of poly(lactic acid) (PLA) composites with cellulose micro fibres (CF) was investigated.
26686158	1	35	theme	lactic	275:280	arg1	acid					282:285	lactic acid	275:285	lactic acid	275:285	The effect of addition of an ethylene-vinyl acetate copolymer modified with glycidyl methacrylate (EVA-GMA) on the structure and properties of poly(lactic acid) (PLA) composites with cellulose micro fibres (CF) was investigated.
26686158	3	36	theme	blends	562:567	arg1	miscibility					520:530	miscibility	520:530	miscibility	520:530	The miscibility and morphology of PLA/EVA-GMA blends were first examined as a function of composition: a large rise of PLA spherulite growth rate in the blends was discovered with increasing the EVA-GMA content (0-30 wt%) in the isothermal crystallization both from the melt and the solid state.
26686158	3	36	theme	blends	562:567	arg1	function					594:601	a function	592:601	a function of composition	592:616	The miscibility and morphology of PLA/EVA-GMA blends were first examined as a function of composition: a large rise of PLA spherulite growth rate in the blends was discovered with increasing the EVA-GMA content (0-30 wt%) in the isothermal crystallization both from the melt and the solid state.
26686158	3	36	theme	blends	562:567	arg1	morphology					536:545	morphology	536:545	morphology	536:545	The miscibility and morphology of PLA/EVA-GMA blends were first examined as a function of composition: a large rise of PLA spherulite growth rate in the blends was discovered with increasing the EVA-GMA content (0-30 wt%) in the isothermal crystallization both from the melt and the solid state.
26686158	1	37	theme	glycidyl	203:210	arg1	EVA-GMA					226:232	EVA-GMA	226:232	EVA-GMA	226:232	The effect of addition of an ethylene-vinyl acetate copolymer modified with glycidyl methacrylate (EVA-GMA) on the structure and properties of poly(lactic acid) (PLA) composites with cellulose micro fibres (CF) was investigated.
26686158	1	37	theme	glycidyl	203:210	arg1	methacrylate					212:223	glycidyl methacrylate	203:223	glycidyl methacrylate (EVA-GMA)	203:233	The effect of addition of an ethylene-vinyl acetate copolymer modified with glycidyl methacrylate (EVA-GMA) on the structure and properties of poly(lactic acid) (PLA) composites with cellulose micro fibres (CF) was investigated.
26686158	0	38	theme	lactic	24:29	arg1	poly					19:22	poly	19:22	poly(lactic acid)/cellulose	19:45	Property tuning of poly(lactic acid)/cellulose bio-composites through blending with modified ethylene-vinyl acetate copolymer.
26686158	0	38	theme	lactic	24:29	arg1	acid					31:34	lactic acid	24:34	lactic acid	24:34	Property tuning of poly(lactic acid)/cellulose bio-composites through blending with modified ethylene-vinyl acetate copolymer.
26686158	3	39	theme	spherulite	639:648	arg1	rate					657:660	PLA spherulite growth rate	635:660	PLA spherulite growth rate	635:660	The miscibility and morphology of PLA/EVA-GMA blends were first examined as a function of composition: a large rise of PLA spherulite growth rate in the blends was discovered with increasing the EVA-GMA content (0-30 wt%) in the isothermal crystallization both from the melt and the solid state.
26686158	2	40	from	mixing	429:434	arg1	mixer					449:453	Brabender mixer	439:453	Brabender mixer	439:453	Binary (PLA/CF) and ternary (PLA/EVA-GMA/CF) composites obtained by melt mixing in Brabender mixer were analysed by SEM, POM, WAXS, DSC, TGA and tensile tests.
26686158	3	41	theme	solid	799:803	arg1	state					805:809	the solid state	795:809	the solid state	795:809	The miscibility and morphology of PLA/EVA-GMA blends were first examined as a function of composition: a large rise of PLA spherulite growth rate in the blends was discovered with increasing the EVA-GMA content (0-30 wt%) in the isothermal crystallization both from the melt and the solid state.
26686158	2	42	theme	Binary	356:361	arg1	composites					401:410	Binary (PLA/CF) and ternary (PLA/EVA-GMA/CF) composites	356:410	Binary (PLA/CF) and ternary (PLA/EVA-GMA/CF) composites obtained by melt mixing in Brabender mixer	356:453	Binary (PLA/CF) and ternary (PLA/EVA-GMA/CF) composites obtained by melt mixing in Brabender mixer were analysed by SEM, POM, WAXS, DSC, TGA and tensile tests.
26686158	5	43	theme	thermodynamic	963:975	arg1	parameters					989:998	thermodynamic and tensile parameters	963:998	parameters	989:998	Marked changes of thermodynamic and tensile parameters, as elastic modulus, strength and elongation at break were observed for the composites, depending on blend composition, polymer miscibility and fibre-matrix chemical interactions at the interface.
26686158	3	44	from	rise	627:630	arg1	blends					669:674	the blends	665:674	the blends	665:674	The miscibility and morphology of PLA/EVA-GMA blends were first examined as a function of composition: a large rise of PLA spherulite growth rate in the blends was discovered with increasing the EVA-GMA content (0-30 wt%) in the isothermal crystallization both from the melt and the solid state.
26686158	4	45	theme	ternary	827:833	arg1	composites					835:844	PLA/EVA-GMA/CF ternary composites	812:844	PLA/EVA-GMA/CF ternary composites	812:844	PLA/EVA-GMA/CF ternary composites displayed improved adhesion and dispersion of fibres into the matrix as compared to PLA/CF system.
26686158	0	46	theme	/cellulose	36:45	arg1	tuning					9:14	Property tuning	0:14	Property tuning of poly(lactic acid)/cellulose	0:45	Property tuning of poly(lactic acid)/cellulose bio-composites through blending with modified ethylene-vinyl acetate copolymer.
26686158	3	47	theme	composition	606:616	arg1	miscibility					520:530	miscibility	520:530	miscibility	520:530	The miscibility and morphology of PLA/EVA-GMA blends were first examined as a function of composition: a large rise of PLA spherulite growth rate in the blends was discovered with increasing the EVA-GMA content (0-30 wt%) in the isothermal crystallization both from the melt and the solid state.
26686158	3	47	theme	composition	606:616	arg1	function					594:601	a function	592:601	a function of composition	592:616	The miscibility and morphology of PLA/EVA-GMA blends were first examined as a function of composition: a large rise of PLA spherulite growth rate in the blends was discovered with increasing the EVA-GMA content (0-30 wt%) in the isothermal crystallization both from the melt and the solid state.
26686158	3	47	theme	composition	606:616	arg1	morphology					536:545	morphology	536:545	morphology	536:545	The miscibility and morphology of PLA/EVA-GMA blends were first examined as a function of composition: a large rise of PLA spherulite growth rate in the blends was discovered with increasing the EVA-GMA content (0-30 wt%) in the isothermal crystallization both from the melt and the solid state.
26686158	2	48	dep	ternary	376:382	arg1	PLA/EVA-GMA/CF					385:398	PLA/EVA-GMA/CF	385:398	PLA/EVA-GMA/CF	385:398	Binary (PLA/CF) and ternary (PLA/EVA-GMA/CF) composites obtained by melt mixing in Brabender mixer were analysed by SEM, POM, WAXS, DSC, TGA and tensile tests.
26686158	5	49	theme	Marked	945:950	arg1	modulus					1012:1018	elastic modulus	1004:1018	elastic modulus	1004:1018	Marked changes of thermodynamic and tensile parameters, as elastic modulus, strength and elongation at break were observed for the composites, depending on blend composition, polymer miscibility and fibre-matrix chemical interactions at the interface.
26686158	5	49	theme	Marked	945:950	arg1	elongation					1034:1043	elongation	1034:1043	elongation	1034:1043	Marked changes of thermodynamic and tensile parameters, as elastic modulus, strength and elongation at break were observed for the composites, depending on blend composition, polymer miscibility and fibre-matrix chemical interactions at the interface.
26686158	5	49	theme	Marked	945:950	arg1	changes					952:958	Marked changes	945:958	Marked changes of thermodynamic and tensile parameters	945:998	Marked changes of thermodynamic and tensile parameters, as elastic modulus, strength and elongation at break were observed for the composites, depending on blend composition, polymer miscibility and fibre-matrix chemical interactions at the interface.
26686158	5	49	theme	Marked	945:950	arg1	strength					1021:1028	strength	1021:1028	strength	1021:1028	Marked changes of thermodynamic and tensile parameters, as elastic modulus, strength and elongation at break were observed for the composites, depending on blend composition, polymer miscibility and fibre-matrix chemical interactions at the interface.
26686158	5	50	theme	blend	1101:1105	arg1	composition					1107:1117	blend composition	1101:1117	blend composition	1101:1117	Marked changes of thermodynamic and tensile parameters, as elastic modulus, strength and elongation at break were observed for the composites, depending on blend composition, polymer miscibility and fibre-matrix chemical interactions at the interface.
26686158	3	51	theme	wt	733:734	arg1	content					719:725	the EVA-GMA content	707:725	the EVA-GMA content (0-30 wt%)	707:736	The miscibility and morphology of PLA/EVA-GMA blends were first examined as a function of composition: a large rise of PLA spherulite growth rate in the blends was discovered with increasing the EVA-GMA content (0-30 wt%) in the isothermal crystallization both from the melt and the solid state.
26686158	3	51	theme	wt	733:734	arg1	%					735:735	0-30 wt%	728:735	0-30 wt%	728:735	The miscibility and morphology of PLA/EVA-GMA blends were first examined as a function of composition: a large rise of PLA spherulite growth rate in the blends was discovered with increasing the EVA-GMA content (0-30 wt%) in the isothermal crystallization both from the melt and the solid state.
26686158	5	52	theme	chemical	1157:1164	arg1	interactions					1166:1177	fibre-matrix chemical interactions	1144:1177	fibre-matrix chemical interactions at the interface	1144:1194	Marked changes of thermodynamic and tensile parameters, as elastic modulus, strength and elongation at break were observed for the composites, depending on blend composition, polymer miscibility and fibre-matrix chemical interactions at the interface.
26686158	2	53	dep	Binary	356:361	arg1	PLA/CF					364:369	PLA/CF	364:369	PLA/CF	364:369	Binary (PLA/CF) and ternary (PLA/EVA-GMA/CF) composites obtained by melt mixing in Brabender mixer were analysed by SEM, POM, WAXS, DSC, TGA and tensile tests.
26686158	4	54	theme	improved	856:863	arg1	adhesion					865:872	improved adhesion	856:872	improved adhesion	856:872	PLA/EVA-GMA/CF ternary composites displayed improved adhesion and dispersion of fibres into the matrix as compared to PLA/CF system.
26686158	2	55	theme	Brabender	439:447	arg1	mixer					449:453	Brabender mixer	439:453	Brabender mixer	439:453	Binary (PLA/CF) and ternary (PLA/EVA-GMA/CF) composites obtained by melt mixing in Brabender mixer were analysed by SEM, POM, WAXS, DSC, TGA and tensile tests.
26686158	1	56	theme	composites	294:303	arg1	properties					256:265	properties	256:265	properties	256:265	The effect of addition of an ethylene-vinyl acetate copolymer modified with glycidyl methacrylate (EVA-GMA) on the structure and properties of poly(lactic acid) (PLA) composites with cellulose micro fibres (CF) was investigated.
26686158	1	56	theme	composites	294:303	arg1	structure					242:250	the structure	238:250	the structure	238:250	The effect of addition of an ethylene-vinyl acetate copolymer modified with glycidyl methacrylate (EVA-GMA) on the structure and properties of poly(lactic acid) (PLA) composites with cellulose micro fibres (CF) was investigated.
26686158	1	57	theme	addition	141:148	arg1	effect					131:136	The effect	127:136	The effect of addition of an ethylene-vinyl acetate copolymer modified with glycidyl methacrylate (EVA-GMA) on the structure and properties of poly(lactic acid) (PLA) composites with cellulose micro fibres (CF)	127:336	The effect of addition of an ethylene-vinyl acetate copolymer modified with glycidyl methacrylate (EVA-GMA) on the structure and properties of poly(lactic acid) (PLA) composites with cellulose micro fibres (CF) was investigated.
26686158	5	58	theme	polymer	1120:1126	arg1	miscibility					1128:1138	polymer miscibility	1120:1138	polymer miscibility	1120:1138	Marked changes of thermodynamic and tensile parameters, as elastic modulus, strength and elongation at break were observed for the composites, depending on blend composition, polymer miscibility and fibre-matrix chemical interactions at the interface.
27285578	0	0	theme	green	90:94	arg1	macroalgae					96:105	green macroalgae	90:105	green macroalgae	90:105	Production and characterization of enzymatic cocktail produced by Aspergillus niger using green macroalgae as nitrogen source and its application in the pre-treatment for biogas production from Ulva rigida.
27285578	2	1	theme	polysaccharides	500:514	arg1	conversion					478:487	the conversion	474:487	the conversion of complex polysaccharides to fermentable sugars	474:536	The use of enzymatic hydrolysis may provide new pathways in the conversion of complex polysaccharides to fermentable sugars.
27285578	3	2	dep	macroalgae	656:665	arg1	Ulva					667:670	Ulva	667:670	Ulva	667:670	In this study, an enzymatic cocktail with high specificity was first isolated from Aspergillus niger using the green macroalgae Ulva rigida as nitrogen source.
27285578	6	3	theme	U.	940:941	arg1	pre-treatment					950:962	The U. rigida pre-treatment	936:962	The U. rigida pre-treatment with this enzymatic cocktail	936:991	The U. rigida pre-treatment with this enzymatic cocktail showed high rate of reduced sugar release, and could bring promising prospects for enzymatic pre-treatment of the biogas production from U. rigida biomass which reached 1175mLgCODint(-1).
27285578	3	4	attach	isolated	608:615	arg1	niger					634:638	Aspergillus niger	622:638	Aspergillus niger	622:638	In this study, an enzymatic cocktail with high specificity was first isolated from Aspergillus niger using the green macroalgae Ulva rigida as nitrogen source.
27285578	3	4	attach	isolated	608:615	arg2	cocktail					567:574	an enzymatic cocktail	554:574	an enzymatic cocktail with high specificity	554:596	In this study, an enzymatic cocktail with high specificity was first isolated from Aspergillus niger using the green macroalgae Ulva rigida as nitrogen source.
27285578	2	5	theme	complex	492:498	arg1	polysaccharides					500:514	complex polysaccharides	492:514	complex polysaccharides	492:514	The use of enzymatic hydrolysis may provide new pathways in the conversion of complex polysaccharides to fermentable sugars.
27285578	1	6	theme	macroalgae	349:358	arg1	polysaccharides					325:339	polysaccharides	325:339	polysaccharides of this macroalgae	325:358	Marine macroalgae are gaining more and more importance as a renewable feedstock for durable bioenergy production, but polysaccharides of this macroalgae are structurally complex in its chemical composition.
27285578	6	7	theme	rigida	943:948	arg1	pre-treatment					950:962	The U. rigida pre-treatment	936:962	The U. rigida pre-treatment with this enzymatic cocktail	936:991	The U. rigida pre-treatment with this enzymatic cocktail showed high rate of reduced sugar release, and could bring promising prospects for enzymatic pre-treatment of the biogas production from U. rigida biomass which reached 1175mLgCODint(-1).
27285578	5	8	theme	lowest	907:912	arg1	4.6IUmL					923:929	the lowest activity 4.6IUmL	903:929	the lowest activity 4.6IUmL(-1)	903:933	The highest activity was obtained with β-glucosidase (109IUmL(-1)) and pectinase (76IUmL(-1)), while CMCase present the lowest activity 4.6IUmL(-1).
27285578	5	8	theme	lowest	907:912	arg1	-1					931:932	-1	931:932	-1	931:932	The highest activity was obtained with β-glucosidase (109IUmL(-1)) and pectinase (76IUmL(-1)), while CMCase present the lowest activity 4.6IUmL(-1).
27285578	6	9	theme	production	1114:1123	arg1	pre-treatment					1086:1098	enzymatic pre-treatment	1076:1098	enzymatic pre-treatment of the biogas production from U. rigida biomass	1076:1146	The U. rigida pre-treatment with this enzymatic cocktail showed high rate of reduced sugar release, and could bring promising prospects for enzymatic pre-treatment of the biogas production from U. rigida biomass which reached 1175mLgCODint(-1).
27285578	0	10	theme	Ulva	194:197	arg1	rigida					199:204	Ulva rigida	194:204	Ulva rigida	194:204	Production and characterization of enzymatic cocktail produced by Aspergillus niger using green macroalgae as nitrogen source and its application in the pre-treatment for biogas production from Ulva rigida.
27285578	3	11	theme	enzymatic	557:565	arg1	cocktail					567:574	an enzymatic cocktail	554:574	an enzymatic cocktail with high specificity	554:596	In this study, an enzymatic cocktail with high specificity was first isolated from Aspergillus niger using the green macroalgae Ulva rigida as nitrogen source.
27285578	1	12	theme	renewable	267:275	arg1	feedstock					277:285	a renewable feedstock	265:285	a renewable feedstock for durable bioenergy production	265:318	Marine macroalgae are gaining more and more importance as a renewable feedstock for durable bioenergy production, but polysaccharides of this macroalgae are structurally complex in its chemical composition.
27285578	1	12	theme	renewable	267:275	arg1	importance					251:260	more and more importance	237:260	more and more importance	237:260	Marine macroalgae are gaining more and more importance as a renewable feedstock for durable bioenergy production, but polysaccharides of this macroalgae are structurally complex in its chemical composition.
27285578	2	13	theme	enzymatic	425:433	arg1	hydrolysis					435:444	enzymatic hydrolysis	425:444	enzymatic hydrolysis	425:444	The use of enzymatic hydrolysis may provide new pathways in the conversion of complex polysaccharides to fermentable sugars.
27285578	6	14	theme	rigida	1133:1138	arg1	biomass					1140:1146	U. rigida biomass	1130:1146	U. rigida biomass	1130:1146	The U. rigida pre-treatment with this enzymatic cocktail showed high rate of reduced sugar release, and could bring promising prospects for enzymatic pre-treatment of the biogas production from U. rigida biomass which reached 1175mLgCODint(-1).
27285578	0	15	theme	nitrogen	110:117	arg1	source					119:124	nitrogen source	110:124	nitrogen source	110:124	Production and characterization of enzymatic cocktail produced by Aspergillus niger using green macroalgae as nitrogen source and its application in the pre-treatment for biogas production from Ulva rigida.
27285578	1	16	theme	more	237:240	arg1	feedstock					277:285	a renewable feedstock	265:285	a renewable feedstock for durable bioenergy production	265:318	Marine macroalgae are gaining more and more importance as a renewable feedstock for durable bioenergy production, but polysaccharides of this macroalgae are structurally complex in its chemical composition.
27285578	1	16	theme	more	237:240	arg1	importance					251:260	more and more importance	237:260	more and more importance	237:260	Marine macroalgae are gaining more and more importance as a renewable feedstock for durable bioenergy production, but polysaccharides of this macroalgae are structurally complex in its chemical composition.
27285578	3	17	with	cocktail	567:574	arg1	specificity					586:596	high specificity	581:596	high specificity	581:596	In this study, an enzymatic cocktail with high specificity was first isolated from Aspergillus niger using the green macroalgae Ulva rigida as nitrogen source.
27285578	5	18	theme	highest	791:797	arg1	activity					799:806	The highest activity	787:806	The highest activity	787:806	The highest activity was obtained with β-glucosidase (109IUmL(-1)) and pectinase (76IUmL(-1)), while CMCase present the lowest activity 4.6IUmL(-1).
27285578	3	19	theme	high	581:584	arg1	specificity					586:596	high specificity	581:596	high specificity	581:596	In this study, an enzymatic cocktail with high specificity was first isolated from Aspergillus niger using the green macroalgae Ulva rigida as nitrogen source.
27285578	6	20	from	biomass	1140:1146	arg1	production					1114:1123	the biogas production	1103:1123	the biogas production from U. rigida biomass	1103:1146	The U. rigida pre-treatment with this enzymatic cocktail showed high rate of reduced sugar release, and could bring promising prospects for enzymatic pre-treatment of the biogas production from U. rigida biomass which reached 1175mLgCODint(-1).
27285578	6	20	from	biomass	1140:1146	arg1	pre-treatment					1086:1098	enzymatic pre-treatment	1076:1098	enzymatic pre-treatment of the biogas production from U. rigida biomass	1076:1146	The U. rigida pre-treatment with this enzymatic cocktail showed high rate of reduced sugar release, and could bring promising prospects for enzymatic pre-treatment of the biogas production from U. rigida biomass which reached 1175mLgCODint(-1).
27285578	6	21	theme	release	1027:1033	arg1	rate					1005:1008	high rate	1000:1008	high rate of reduced sugar release	1000:1033	The U. rigida pre-treatment with this enzymatic cocktail showed high rate of reduced sugar release, and could bring promising prospects for enzymatic pre-treatment of the biogas production from U. rigida biomass which reached 1175mLgCODint(-1).
27285578	2	22	theme	new	458:460	arg1	pathways					462:469	new pathways	458:469	new pathways	458:469	The use of enzymatic hydrolysis may provide new pathways in the conversion of complex polysaccharides to fermentable sugars.
27285578	0	23	from	rigida	199:204	arg1	production					178:187	biogas production	171:187	biogas production from Ulva rigida	171:204	Production and characterization of enzymatic cocktail produced by Aspergillus niger using green macroalgae as nitrogen source and its application in the pre-treatment for biogas production from Ulva rigida.
27285578	5	24	theme	activity	914:921	arg1	4.6IUmL					923:929	the lowest activity 4.6IUmL	903:929	the lowest activity 4.6IUmL(-1)	903:933	The highest activity was obtained with β-glucosidase (109IUmL(-1)) and pectinase (76IUmL(-1)), while CMCase present the lowest activity 4.6IUmL(-1).
27285578	5	24	theme	activity	914:921	arg1	-1					931:932	-1	931:932	-1	931:932	The highest activity was obtained with β-glucosidase (109IUmL(-1)) and pectinase (76IUmL(-1)), while CMCase present the lowest activity 4.6IUmL(-1).
27285578	6	25	theme	sugar	1021:1025	arg1	release					1027:1033	reduced sugar release	1013:1033	reduced sugar release	1013:1033	The U. rigida pre-treatment with this enzymatic cocktail showed high rate of reduced sugar release, and could bring promising prospects for enzymatic pre-treatment of the biogas production from U. rigida biomass which reached 1175mLgCODint(-1).
27285578	6	26	theme	biogas	1107:1112	arg1	production					1114:1123	the biogas production	1103:1123	the biogas production from U. rigida biomass	1103:1146	The U. rigida pre-treatment with this enzymatic cocktail showed high rate of reduced sugar release, and could bring promising prospects for enzymatic pre-treatment of the biogas production from U. rigida biomass which reached 1175mLgCODint(-1).
27285578	2	27	theme	hydrolysis	435:444	arg1	use					418:420	The use	414:420	The use of enzymatic hydrolysis	414:444	The use of enzymatic hydrolysis may provide new pathways in the conversion of complex polysaccharides to fermentable sugars.
27285578	1	28	from	complex	377:383	arg1	composition					401:411	its chemical composition	388:411	its chemical composition	388:411	Marine macroalgae are gaining more and more importance as a renewable feedstock for durable bioenergy production, but polysaccharides of this macroalgae are structurally complex in its chemical composition.
27285578	1	29	theme	durable	291:297	arg1	production					309:318	durable bioenergy production	291:318	durable bioenergy production	291:318	Marine macroalgae are gaining more and more importance as a renewable feedstock for durable bioenergy production, but polysaccharides of this macroalgae are structurally complex in its chemical composition.
27285578	1	30	theme	chemical	392:399	arg1	composition					401:411	its chemical composition	388:411	its chemical composition	388:411	Marine macroalgae are gaining more and more importance as a renewable feedstock for durable bioenergy production, but polysaccharides of this macroalgae are structurally complex in its chemical composition.
27285578	1	31	theme	Marine	207:212	arg1	macroalgae					214:223	Marine macroalgae	207:223	Marine macroalgae	207:223	Marine macroalgae are gaining more and more importance as a renewable feedstock for durable bioenergy production, but polysaccharides of this macroalgae are structurally complex in its chemical composition.
27285578	0	32	theme	cocktail	45:52	arg1	characterization					15:30	characterization	15:30	characterization	15:30	Production and characterization of enzymatic cocktail produced by Aspergillus niger using green macroalgae as nitrogen source and its application in the pre-treatment for biogas production from Ulva rigida.
27285578	0	32	theme	cocktail	45:52	arg1	Production					0:9	Production	0:9	Production	0:9	Production and characterization of enzymatic cocktail produced by Aspergillus niger using green macroalgae as nitrogen source and its application in the pre-treatment for biogas production from Ulva rigida.
27285578	1	33	theme	bioenergy	299:307	arg1	production					309:318	durable bioenergy production	291:318	durable bioenergy production	291:318	Marine macroalgae are gaining more and more importance as a renewable feedstock for durable bioenergy production, but polysaccharides of this macroalgae are structurally complex in its chemical composition.
27285578	0	34	theme	enzymatic	35:43	arg1	cocktail					45:52	enzymatic cocktail	35:52	enzymatic cocktail	35:52	Production and characterization of enzymatic cocktail produced by Aspergillus niger using green macroalgae as nitrogen source and its application in the pre-treatment for biogas production from Ulva rigida.
27285578	3	35	theme	green	650:654	arg1	rigida					672:677	the green macroalgae Ulva rigida	646:677	the green macroalgae Ulva rigida	646:677	In this study, an enzymatic cocktail with high specificity was first isolated from Aspergillus niger using the green macroalgae Ulva rigida as nitrogen source.
27285578	1	36	from	composition	401:411	arg1	complex					377:383	complex	377:383	complex	377:383	Marine macroalgae are gaining more and more importance as a renewable feedstock for durable bioenergy production, but polysaccharides of this macroalgae are structurally complex in its chemical composition.
27285578	6	37	theme	high	1000:1003	arg1	rate					1005:1008	high rate	1000:1008	high rate of reduced sugar release	1000:1033	The U. rigida pre-treatment with this enzymatic cocktail showed high rate of reduced sugar release, and could bring promising prospects for enzymatic pre-treatment of the biogas production from U. rigida biomass which reached 1175mLgCODint(-1).
27285578	3	38	theme	macroalgae	656:665	arg1	rigida					672:677	the green macroalgae Ulva rigida	646:677	the green macroalgae Ulva rigida	646:677	In this study, an enzymatic cocktail with high specificity was first isolated from Aspergillus niger using the green macroalgae Ulva rigida as nitrogen source.
27285578	0	39	from	source	119:124	arg1	pre-treatment					153:165	the pre-treatment	149:165	the pre-treatment	149:165	Production and characterization of enzymatic cocktail produced by Aspergillus niger using green macroalgae as nitrogen source and its application in the pre-treatment for biogas production from Ulva rigida.
27285578	0	40	theme	biogas	171:176	arg1	production					178:187	biogas production	171:187	biogas production from Ulva rigida	171:204	Production and characterization of enzymatic cocktail produced by Aspergillus niger using green macroalgae as nitrogen source and its application in the pre-treatment for biogas production from Ulva rigida.
27285578	6	41	from	pre-treatment	1086:1098	arg1	biomass					1140:1146	U. rigida biomass	1130:1146	U. rigida biomass	1130:1146	The U. rigida pre-treatment with this enzymatic cocktail showed high rate of reduced sugar release, and could bring promising prospects for enzymatic pre-treatment of the biogas production from U. rigida biomass which reached 1175mLgCODint(-1).
27285578	6	42	theme	enzymatic	1076:1084	arg1	pre-treatment					1086:1098	enzymatic pre-treatment	1076:1098	enzymatic pre-treatment of the biogas production from U. rigida biomass	1076:1146	The U. rigida pre-treatment with this enzymatic cocktail showed high rate of reduced sugar release, and could bring promising prospects for enzymatic pre-treatment of the biogas production from U. rigida biomass which reached 1175mLgCODint(-1).
27285578	6	43	theme	U.	1130:1131	arg1	biomass					1140:1146	U. rigida biomass	1130:1146	U. rigida biomass	1130:1146	The U. rigida pre-treatment with this enzymatic cocktail showed high rate of reduced sugar release, and could bring promising prospects for enzymatic pre-treatment of the biogas production from U. rigida biomass which reached 1175mLgCODint(-1).
27285578	0	44	from	application	134:144	arg1	pre-treatment					153:165	the pre-treatment	149:165	the pre-treatment	149:165	Production and characterization of enzymatic cocktail produced by Aspergillus niger using green macroalgae as nitrogen source and its application in the pre-treatment for biogas production from Ulva rigida.
27285578	2	45	theme	fermentable	519:529	arg1	sugars					531:536	fermentable sugars	519:536	fermentable sugars	519:536	The use of enzymatic hydrolysis may provide new pathways in the conversion of complex polysaccharides to fermentable sugars.
27285578	3	46	theme	nitrogen	682:689	arg1	source					691:696	nitrogen source	682:696	nitrogen source	682:696	In this study, an enzymatic cocktail with high specificity was first isolated from Aspergillus niger using the green macroalgae Ulva rigida as nitrogen source.
27285578	3	47	theme	Aspergillus	622:632	arg1	niger					634:638	Aspergillus niger	622:638	Aspergillus niger	622:638	In this study, an enzymatic cocktail with high specificity was first isolated from Aspergillus niger using the green macroalgae Ulva rigida as nitrogen source.
27285578	6	48	theme	reduced	1013:1019	arg1	release					1027:1033	reduced sugar release	1013:1033	reduced sugar release	1013:1033	The U. rigida pre-treatment with this enzymatic cocktail showed high rate of reduced sugar release, and could bring promising prospects for enzymatic pre-treatment of the biogas production from U. rigida biomass which reached 1175mLgCODint(-1).
27285578	6	49	theme	enzymatic	974:982	arg1	cocktail					984:991	this enzymatic cocktail	969:991	this enzymatic cocktail	969:991	The U. rigida pre-treatment with this enzymatic cocktail showed high rate of reduced sugar release, and could bring promising prospects for enzymatic pre-treatment of the biogas production from U. rigida biomass which reached 1175mLgCODint(-1).
27285578	0	50	theme	Aspergillus	66:76	arg1	niger					78:82	Aspergillus niger	66:82	Aspergillus niger	66:82	Production and characterization of enzymatic cocktail produced by Aspergillus niger using green macroalgae as nitrogen source and its application in the pre-treatment for biogas production from Ulva rigida.
27285578	6	51	with	pre-treatment	950:962	arg1	cocktail					984:991	this enzymatic cocktail	969:991	this enzymatic cocktail	969:991	The U. rigida pre-treatment with this enzymatic cocktail showed high rate of reduced sugar release, and could bring promising prospects for enzymatic pre-treatment of the biogas production from U. rigida biomass which reached 1175mLgCODint(-1).
27285578	6	52	theme	promising	1052:1060	arg1	prospects					1062:1070	promising prospects	1052:1070	promising prospects for enzymatic pre-treatment of the biogas production from U. rigida biomass which reached 1175mLgCODint(-1)	1052:1178	The U. rigida pre-treatment with this enzymatic cocktail showed high rate of reduced sugar release, and could bring promising prospects for enzymatic pre-treatment of the biogas production from U. rigida biomass which reached 1175mLgCODint(-1).
27285578	1	53	theme	more	246:249	arg1	feedstock					277:285	a renewable feedstock	265:285	a renewable feedstock for durable bioenergy production	265:318	Marine macroalgae are gaining more and more importance as a renewable feedstock for durable bioenergy production, but polysaccharides of this macroalgae are structurally complex in its chemical composition.
27285578	1	53	theme	more	246:249	arg1	importance					251:260	more and more importance	237:260	more and more importance	237:260	Marine macroalgae are gaining more and more importance as a renewable feedstock for durable bioenergy production, but polysaccharides of this macroalgae are structurally complex in its chemical composition.
27185120	5	0	theme	in	717:718	arg1	nanofibers					747:756	The in situ cross-linked CMCS-ADA nanofibers	713:756	The in situ cross-linked CMCS-ADA nanofibers	713:756	The in situ cross-linked CMCS-ADA nanofibers were then obtained following the extraction of water-soluble PEO.
27185120	6	1	from	pH	880:881	arg1	PBS					870:872	PBS	870:872	PBS	870:872	After immersion in phosphate-buffered saline (PBS) at a pH of 7.4 for up to 15days, the as-spun CMCS-ADA-PEO composite nanofibers maintained structural integrity, confirming the success of the crosslinking.
27185120	6	1	from	pH	880:881	arg1	immersion					830:838	immersion	830:838	immersion in phosphate-buffered saline (PBS) at a pH of 7.4 for up to 15days	830:905	After immersion in phosphate-buffered saline (PBS) at a pH of 7.4 for up to 15days, the as-spun CMCS-ADA-PEO composite nanofibers maintained structural integrity, confirming the success of the crosslinking.
27185120	6	1	from	pH	880:881	arg1	saline					862:867	phosphate-buffered saline	843:867	phosphate-buffered saline (PBS) at a pH of 7.4 for up to 15days	843:905	After immersion in phosphate-buffered saline (PBS) at a pH of 7.4 for up to 15days, the as-spun CMCS-ADA-PEO composite nanofibers maintained structural integrity, confirming the success of the crosslinking.
27185120	1	2	with	process	260:266	arg1	the					273:275	the	273:275	the	273:275	We have prepared carboxymethyl chitosan-alginate dialdehyde (CMCS-ADA) nanofibers via a reactive electrospinning process with the aid of polyethylene oxide (PEO).
27185120	1	3	theme	carboxymethyl	164:176	arg1	nanofibers					218:227	carboxymethyl chitosan-alginate dialdehyde (CMCS-ADA) nanofibers	164:227	carboxymethyl chitosan-alginate dialdehyde (CMCS-ADA) nanofibers	164:227	We have prepared carboxymethyl chitosan-alginate dialdehyde (CMCS-ADA) nanofibers via a reactive electrospinning process with the aid of polyethylene oxide (PEO).
27185120	0	4	theme	alginate	91:98	arg1	dialdehyde					100:109	alginate dialdehyde	91:109	alginate dialdehyde with the aid of polyethylene oxide	91:144	Reactive electrospinning of composite nanofibers of carboxymethyl chitosan cross-linked by alginate dialdehyde with the aid of polyethylene oxide.
27185120	0	5	with	dialdehyde	100:109	arg1	the					116:118	the	116:118	the	116:118	Reactive electrospinning of composite nanofibers of carboxymethyl chitosan cross-linked by alginate dialdehyde with the aid of polyethylene oxide.
27185120	1	6	theme	chitosan-alginate	178:194	arg1	nanofibers					218:227	carboxymethyl chitosan-alginate dialdehyde (CMCS-ADA) nanofibers	164:227	carboxymethyl chitosan-alginate dialdehyde (CMCS-ADA) nanofibers	164:227	We have prepared carboxymethyl chitosan-alginate dialdehyde (CMCS-ADA) nanofibers via a reactive electrospinning process with the aid of polyethylene oxide (PEO).
27185120	6	7	theme	composite	933:941	arg1	nanofibers					943:952	the as-spun CMCS-ADA-PEO composite nanofibers	908:952	the as-spun CMCS-ADA-PEO composite nanofibers	908:952	After immersion in phosphate-buffered saline (PBS) at a pH of 7.4 for up to 15days, the as-spun CMCS-ADA-PEO composite nanofibers maintained structural integrity, confirming the success of the crosslinking.
27185120	7	8	dep	adhesion	1082:1089	arg1	the					1078:1080	the	1078:1080	the	1078:1080	The PEO-extracted CMCS-ADA nanofibers promoted the adhesion, proliferation and alkaline phosphatase activity of bone marrow stromal cells.
27185120	1	9	theme	polyethylene	284:295	arg1	PEO					304:306	PEO	304:306	PEO	304:306	We have prepared carboxymethyl chitosan-alginate dialdehyde (CMCS-ADA) nanofibers via a reactive electrospinning process with the aid of polyethylene oxide (PEO).
27185120	1	9	theme	polyethylene	284:295	arg1	oxide					297:301	polyethylene oxide	284:301	polyethylene oxide (PEO)	284:307	We have prepared carboxymethyl chitosan-alginate dialdehyde (CMCS-ADA) nanofibers via a reactive electrospinning process with the aid of polyethylene oxide (PEO).
27185120	3	10	theme	gel	532:534	arg1	formation					536:544	the gel formation	528:544	the gel formation	528:544	The mixed solution can be adjusted to come out from the needle before the gel formation or when the gel was just about to form.
27185120	6	11	theme	as-spun	912:918	arg1	nanofibers					943:952	the as-spun CMCS-ADA-PEO composite nanofibers	908:952	the as-spun CMCS-ADA-PEO composite nanofibers	908:952	After immersion in phosphate-buffered saline (PBS) at a pH of 7.4 for up to 15days, the as-spun CMCS-ADA-PEO composite nanofibers maintained structural integrity, confirming the success of the crosslinking.
27185120	5	12	theme	water-soluble	805:817	arg1	PEO					819:821	water-soluble PEO	805:821	water-soluble PEO	805:821	The in situ cross-linked CMCS-ADA nanofibers were then obtained following the extraction of water-soluble PEO.
27185120	7	13	theme	cells	1163:1167	arg1	adhesion					1082:1089	adhesion	1082:1089	adhesion	1082:1089	The PEO-extracted CMCS-ADA nanofibers promoted the adhesion, proliferation and alkaline phosphatase activity of bone marrow stromal cells.
27185120	7	13	theme	cells	1163:1167	arg1	activity					1131:1138	alkaline phosphatase activity	1110:1138	alkaline phosphatase activity	1110:1138	The PEO-extracted CMCS-ADA nanofibers promoted the adhesion, proliferation and alkaline phosphatase activity of bone marrow stromal cells.
27185120	7	13	theme	cells	1163:1167	arg1	proliferation					1092:1104	proliferation	1092:1104	proliferation	1092:1104	The PEO-extracted CMCS-ADA nanofibers promoted the adhesion, proliferation and alkaline phosphatase activity of bone marrow stromal cells.
27185120	7	14	theme	bone	1143:1146	arg1	marrow					1148:1153	bone marrow	1143:1153	bone marrow stromal cells	1143:1167	The PEO-extracted CMCS-ADA nanofibers promoted the adhesion, proliferation and alkaline phosphatase activity of bone marrow stromal cells.
27185120	0	15	dep	the	116:118	arg1	aid					120:122	aid	120:122	aid	120:122	Reactive electrospinning of composite nanofibers of carboxymethyl chitosan cross-linked by alginate dialdehyde with the aid of polyethylene oxide.
27185120	6	16	theme	crosslinking	1017:1028	arg1	success					1002:1008	the success	998:1008	the success of the crosslinking	998:1028	After immersion in phosphate-buffered saline (PBS) at a pH of 7.4 for up to 15days, the as-spun CMCS-ADA-PEO composite nanofibers maintained structural integrity, confirming the success of the crosslinking.
27185120	1	17	theme	dialdehyde	196:205	arg1	nanofibers					218:227	carboxymethyl chitosan-alginate dialdehyde (CMCS-ADA) nanofibers	164:227	carboxymethyl chitosan-alginate dialdehyde (CMCS-ADA) nanofibers	164:227	We have prepared carboxymethyl chitosan-alginate dialdehyde (CMCS-ADA) nanofibers via a reactive electrospinning process with the aid of polyethylene oxide (PEO).
27185120	2	18	theme	ADA-PEO	432:438	arg1	solution					448:455	ADA-PEO blended solution	432:455	ADA-PEO blended solution	432:455	The presence of PEO delayed the gelation of CMCS and ADA, thus providing ease of use to adjust the mixing of CMCS-PEO and ADA-PEO blended solution.
27185120	7	19	theme	phosphatase	1119:1129	arg1	activity					1131:1138	alkaline phosphatase activity	1110:1138	alkaline phosphatase activity	1110:1138	The PEO-extracted CMCS-ADA nanofibers promoted the adhesion, proliferation and alkaline phosphatase activity of bone marrow stromal cells.
27185120	2	20	theme	CMCS-PEO	419:426	arg1	mixing					409:414	the mixing	405:414	the mixing of CMCS-PEO and ADA-PEO blended solution	405:455	The presence of PEO delayed the gelation of CMCS and ADA, thus providing ease of use to adjust the mixing of CMCS-PEO and ADA-PEO blended solution.
27185120	7	21	theme	marrow	1148:1153	arg1	cells					1163:1167	bone marrow stromal cells	1143:1167	bone marrow stromal cells	1143:1167	The PEO-extracted CMCS-ADA nanofibers promoted the adhesion, proliferation and alkaline phosphatase activity of bone marrow stromal cells.
27185120	6	22	theme	phosphate-buffered	843:860	arg1	PBS					870:872	PBS	870:872	PBS	870:872	After immersion in phosphate-buffered saline (PBS) at a pH of 7.4 for up to 15days, the as-spun CMCS-ADA-PEO composite nanofibers maintained structural integrity, confirming the success of the crosslinking.
27185120	6	22	theme	phosphate-buffered	843:860	arg1	saline					862:867	phosphate-buffered saline	843:867	phosphate-buffered saline (PBS) at a pH of 7.4 for up to 15days	843:905	After immersion in phosphate-buffered saline (PBS) at a pH of 7.4 for up to 15days, the as-spun CMCS-ADA-PEO composite nanofibers maintained structural integrity, confirming the success of the crosslinking.
27185120	7	23	theme	PEO-extracted	1035:1047	arg1	nanofibers					1058:1067	The PEO-extracted CMCS-ADA nanofibers	1031:1067	The PEO-extracted CMCS-ADA nanofibers	1031:1067	The PEO-extracted CMCS-ADA nanofibers promoted the adhesion, proliferation and alkaline phosphatase activity of bone marrow stromal cells.
27185120	2	24	theme	PEO	326:328	arg1	presence					314:321	The presence	310:321	The presence of PEO	310:328	The presence of PEO delayed the gelation of CMCS and ADA, thus providing ease of use to adjust the mixing of CMCS-PEO and ADA-PEO blended solution.
27185120	1	25	theme	CMCS-ADA	208:215	arg1	nanofibers					218:227	carboxymethyl chitosan-alginate dialdehyde (CMCS-ADA) nanofibers	164:227	carboxymethyl chitosan-alginate dialdehyde (CMCS-ADA) nanofibers	164:227	We have prepared carboxymethyl chitosan-alginate dialdehyde (CMCS-ADA) nanofibers via a reactive electrospinning process with the aid of polyethylene oxide (PEO).
27185120	0	26	theme	Reactive	0:7	arg1	electrospinning					9:23	Reactive electrospinning	0:23	Reactive electrospinning of composite nanofibers of carboxymethyl chitosan	0:73	Reactive electrospinning of composite nanofibers of carboxymethyl chitosan cross-linked by alginate dialdehyde with the aid of polyethylene oxide.
27185120	7	27	theme	CMCS-ADA	1049:1056	arg1	nanofibers					1058:1067	The PEO-extracted CMCS-ADA nanofibers	1031:1067	The PEO-extracted CMCS-ADA nanofibers	1031:1067	The PEO-extracted CMCS-ADA nanofibers promoted the adhesion, proliferation and alkaline phosphatase activity of bone marrow stromal cells.
27185120	4	28	theme	average	627:633	arg1	diameters					635:643	average diameters	627:643	average diameters ranging from 100nm to 900nm	627:671	Defect-free CMCS-ADA-PEO nanofibers with average diameters ranging from 100nm to 900nm were obtained using water as a solvent.
27185120	0	29	theme	composite	28:36	arg1	nanofibers					38:47	composite nanofibers	28:47	composite nanofibers of carboxymethyl chitosan	28:73	Reactive electrospinning of composite nanofibers of carboxymethyl chitosan cross-linked by alginate dialdehyde with the aid of polyethylene oxide.
27185120	5	30	dep	in	717:718	arg1	situ					720:723	situ	720:723	situ	720:723	The in situ cross-linked CMCS-ADA nanofibers were then obtained following the extraction of water-soluble PEO.
27185120	6	31	from	immersion	830:838	arg1	PBS					870:872	PBS	870:872	PBS	870:872	After immersion in phosphate-buffered saline (PBS) at a pH of 7.4 for up to 15days, the as-spun CMCS-ADA-PEO composite nanofibers maintained structural integrity, confirming the success of the crosslinking.
27185120	6	31	from	immersion	830:838	arg1	saline					862:867	phosphate-buffered saline	843:867	phosphate-buffered saline (PBS) at a pH of 7.4 for up to 15days	843:905	After immersion in phosphate-buffered saline (PBS) at a pH of 7.4 for up to 15days, the as-spun CMCS-ADA-PEO composite nanofibers maintained structural integrity, confirming the success of the crosslinking.
27185120	6	31	from	immersion	830:838	arg1	pH					880:881	a pH	878:881	a pH of 7.4	878:888	After immersion in phosphate-buffered saline (PBS) at a pH of 7.4 for up to 15days, the as-spun CMCS-ADA-PEO composite nanofibers maintained structural integrity, confirming the success of the crosslinking.
27185120	0	32	theme	polyethylene	127:138	arg1	oxide					140:144	polyethylene oxide	127:144	polyethylene oxide	127:144	Reactive electrospinning of composite nanofibers of carboxymethyl chitosan cross-linked by alginate dialdehyde with the aid of polyethylene oxide.
27185120	2	33	theme	CMCS	354:357	arg1	gelation					342:349	the gelation	338:349	the gelation of CMCS and ADA	338:365	The presence of PEO delayed the gelation of CMCS and ADA, thus providing ease of use to adjust the mixing of CMCS-PEO and ADA-PEO blended solution.
27185120	5	34	theme	cross-linked	725:736	arg1	nanofibers					747:756	The in situ cross-linked CMCS-ADA nanofibers	713:756	The in situ cross-linked CMCS-ADA nanofibers	713:756	The in situ cross-linked CMCS-ADA nanofibers were then obtained following the extraction of water-soluble PEO.
27185120	1	35	dep	the	273:275	arg1	aid					277:279	aid	277:279	aid	277:279	We have prepared carboxymethyl chitosan-alginate dialdehyde (CMCS-ADA) nanofibers via a reactive electrospinning process with the aid of polyethylene oxide (PEO).
27185120	4	36	theme	Defect-free	586:596	arg1	nanofibers					611:620	Defect-free CMCS-ADA-PEO nanofibers	586:620	Defect-free CMCS-ADA-PEO nanofibers with average diameters ranging from 100nm to 900nm	586:671	Defect-free CMCS-ADA-PEO nanofibers with average diameters ranging from 100nm to 900nm were obtained using water as a solvent.
27185120	4	37	theme	CMCS-ADA-PEO	598:609	arg1	nanofibers					611:620	Defect-free CMCS-ADA-PEO nanofibers	586:620	Defect-free CMCS-ADA-PEO nanofibers with average diameters ranging from 100nm to 900nm	586:671	Defect-free CMCS-ADA-PEO nanofibers with average diameters ranging from 100nm to 900nm were obtained using water as a solvent.
27185120	0	38	theme	nanofibers	38:47	arg1	electrospinning					9:23	Reactive electrospinning	0:23	Reactive electrospinning of composite nanofibers of carboxymethyl chitosan	0:73	Reactive electrospinning of composite nanofibers of carboxymethyl chitosan cross-linked by alginate dialdehyde with the aid of polyethylene oxide.
27185120	2	39	theme	solution	448:455	arg1	mixing					409:414	the mixing	405:414	the mixing of CMCS-PEO and ADA-PEO blended solution	405:455	The presence of PEO delayed the gelation of CMCS and ADA, thus providing ease of use to adjust the mixing of CMCS-PEO and ADA-PEO blended solution.
27185120	5	40	theme	PEO	819:821	arg1	extraction					791:800	the extraction	787:800	the extraction of water-soluble PEO	787:821	The in situ cross-linked CMCS-ADA nanofibers were then obtained following the extraction of water-soluble PEO.
27185120	2	41	theme	use	391:393	arg1	ease					383:386	ease	383:386	ease of use	383:393	The presence of PEO delayed the gelation of CMCS and ADA, thus providing ease of use to adjust the mixing of CMCS-PEO and ADA-PEO blended solution.
27185120	0	42	theme	chitosan	66:73	arg1	nanofibers					38:47	composite nanofibers	28:47	composite nanofibers of carboxymethyl chitosan	28:73	Reactive electrospinning of composite nanofibers of carboxymethyl chitosan cross-linked by alginate dialdehyde with the aid of polyethylene oxide.
27185120	4	43	with	nanofibers	611:620	arg1	diameters					635:643	average diameters	627:643	average diameters ranging from 100nm to 900nm	627:671	Defect-free CMCS-ADA-PEO nanofibers with average diameters ranging from 100nm to 900nm were obtained using water as a solvent.
27185120	5	44	link	cross-linked	725:736	arg1	nanofibers					747:756	The in situ cross-linked CMCS-ADA nanofibers	713:756	The in situ cross-linked CMCS-ADA nanofibers	713:756	The in situ cross-linked CMCS-ADA nanofibers were then obtained following the extraction of water-soluble PEO.
27185120	0	45	theme	carboxymethyl	52:64	arg1	chitosan					66:73	carboxymethyl chitosan	52:73	carboxymethyl chitosan	52:73	Reactive electrospinning of composite nanofibers of carboxymethyl chitosan cross-linked by alginate dialdehyde with the aid of polyethylene oxide.
27185120	6	46	theme	7.4	886:888	arg1	pH					880:881	a pH	878:881	a pH of 7.4	878:888	After immersion in phosphate-buffered saline (PBS) at a pH of 7.4 for up to 15days, the as-spun CMCS-ADA-PEO composite nanofibers maintained structural integrity, confirming the success of the crosslinking.
27185120	6	47	theme	CMCS-ADA-PEO	920:931	arg1	nanofibers					943:952	the as-spun CMCS-ADA-PEO composite nanofibers	908:952	the as-spun CMCS-ADA-PEO composite nanofibers	908:952	After immersion in phosphate-buffered saline (PBS) at a pH of 7.4 for up to 15days, the as-spun CMCS-ADA-PEO composite nanofibers maintained structural integrity, confirming the success of the crosslinking.
27185120	7	48	theme	alkaline	1110:1117	arg1	phosphatase					1119:1129	alkaline phosphatase	1110:1129	alkaline phosphatase activity	1110:1138	The PEO-extracted CMCS-ADA nanofibers promoted the adhesion, proliferation and alkaline phosphatase activity of bone marrow stromal cells.
27185120	1	49	theme	electrospinning	244:258	arg1	process					260:266	a reactive electrospinning process	233:266	a reactive electrospinning process with the aid of polyethylene oxide (PEO)	233:307	We have prepared carboxymethyl chitosan-alginate dialdehyde (CMCS-ADA) nanofibers via a reactive electrospinning process with the aid of polyethylene oxide (PEO).
27185120	3	50	theme	mixed	462:466	arg1	solution					468:475	The mixed solution	458:475	The mixed solution	458:475	The mixed solution can be adjusted to come out from the needle before the gel formation or when the gel was just about to form.
27185120	1	51	theme	reactive	235:242	arg1	process					260:266	a reactive electrospinning process	233:266	a reactive electrospinning process with the aid of polyethylene oxide (PEO)	233:307	We have prepared carboxymethyl chitosan-alginate dialdehyde (CMCS-ADA) nanofibers via a reactive electrospinning process with the aid of polyethylene oxide (PEO).
27185120	6	52	theme	structural	965:974	arg1	integrity					976:984	structural integrity	965:984	structural integrity	965:984	After immersion in phosphate-buffered saline (PBS) at a pH of 7.4 for up to 15days, the as-spun CMCS-ADA-PEO composite nanofibers maintained structural integrity, confirming the success of the crosslinking.
27185120	2	53	theme	blended	440:446	arg1	solution					448:455	ADA-PEO blended solution	432:455	ADA-PEO blended solution	432:455	The presence of PEO delayed the gelation of CMCS and ADA, thus providing ease of use to adjust the mixing of CMCS-PEO and ADA-PEO blended solution.
27185120	5	54	theme	CMCS-ADA	738:745	arg1	nanofibers					747:756	The in situ cross-linked CMCS-ADA nanofibers	713:756	The in situ cross-linked CMCS-ADA nanofibers	713:756	The in situ cross-linked CMCS-ADA nanofibers were then obtained following the extraction of water-soluble PEO.
27185120	2	55	theme	ADA	363:365	arg1	gelation					342:349	the gelation	338:349	the gelation of CMCS and ADA	338:365	The presence of PEO delayed the gelation of CMCS and ADA, thus providing ease of use to adjust the mixing of CMCS-PEO and ADA-PEO blended solution.
27185120	7	56	theme	stromal	1155:1161	arg1	cells					1163:1167	bone marrow stromal cells	1143:1167	bone marrow stromal cells	1143:1167	The PEO-extracted CMCS-ADA nanofibers promoted the adhesion, proliferation and alkaline phosphatase activity of bone marrow stromal cells.
28110685	3	0	from	rules	565:569	arg1	pureness					578:585	the pureness	574:585	the pureness of pharmaceutical grade chondrotin sulfate	574:628	Considering the strict regulatory rules on the pureness of pharmaceutical grade chondrotin sulfate there is an urgent need and interest to determine the residual keratan sulfate with specific, sensitive and reliable methods.
28110685	1	1	theme	cartilaginous	280:292	arg1	tissues					294:300	animal cartilaginous tissues	273:300	animal cartilaginous tissues	273:300	Chondroitin sulfate is a glycosaminoglycan widely used as active principle of anti-osteoarthritis drugs and nutraceuticals, manufactured by extraction from animal cartilaginous tissues.
28110685	4	2	theme	developed	886:894	arg1	exclusion					1050:1058	iii) size exclusion	1040:1058	iii) size exclusion	1040:1058	To pursue this aim, in this paper, for the first time, we set up a multi-analytical and preparative approach based on: i) a newly developed method by high performance anion-exchange chromatography with pulsed amperometric detection, ii) gas chromatography-mass spectrometry analyses, iii) size exclusion chromatography analyses coupled with triple detector array module and on iv) strong anion exchange chromatography separation.
28110685	4	2	theme	developed	886:894	arg1	method					896:901	a newly developed method	878:901	: i) a newly developed method by high performance anion-exchange chromatography with pulsed amperometric detection	873:986	To pursue this aim, in this paper, for the first time, we set up a multi-analytical and preparative approach based on: i) a newly developed method by high performance anion-exchange chromatography with pulsed amperometric detection, ii) gas chromatography-mass spectrometry analyses, iii) size exclusion chromatography analyses coupled with triple detector array module and on iv) strong anion exchange chromatography separation.
28110685	4	2	theme	developed	886:894	arg1	analyses					1030:1037	ii) gas chromatography-mass spectrometry analyses	989:1037	ii) gas chromatography-mass spectrometry analyses	989:1037	To pursue this aim, in this paper, for the first time, we set up a multi-analytical and preparative approach based on: i) a newly developed method by high performance anion-exchange chromatography with pulsed amperometric detection, ii) gas chromatography-mass spectrometry analyses, iii) size exclusion chromatography analyses coupled with triple detector array module and on iv) strong anion exchange chromatography separation.
28110685	4	3	theme	triple	1097:1102	arg1	module					1119:1124	triple detector array module	1097:1124	triple detector array module	1097:1124	To pursue this aim, in this paper, for the first time, we set up a multi-analytical and preparative approach based on: i) a newly developed method by high performance anion-exchange chromatography with pulsed amperometric detection, ii) gas chromatography-mass spectrometry analyses, iii) size exclusion chromatography analyses coupled with triple detector array module and on iv) strong anion exchange chromatography separation.
28110685	4	4	dep	analyses	1030:1037	arg1	ii					989:990	ii	989:990	ii	989:990	To pursue this aim, in this paper, for the first time, we set up a multi-analytical and preparative approach based on: i) a newly developed method by high performance anion-exchange chromatography with pulsed amperometric detection, ii) gas chromatography-mass spectrometry analyses, iii) size exclusion chromatography analyses coupled with triple detector array module and on iv) strong anion exchange chromatography separation.
28110685	2	5	theme	manufacturing	314:326	arg1	procedures					328:337	the manufacturing procedures	310:337	the manufacturing procedures	310:337	During the manufacturing procedures, another glycosaminoglycan, the keratan sulfate, might be contemporarily withdrawn, thus eventually constituting a contaminant difficult to be determined because of its structural similarity.
28110685	7	6	with	anion-exchange	1524:1537	arg1	chromatography					1593:1606	size exclusion chromatography	1578:1606	size exclusion chromatography	1578:1606	These peaks analyzed by high performance anion-exchange with pulsed amperometric detection and size exclusion chromatography with triple detector array, ion chromatography and by mono- or two-dimensional nuclear magnetic resonance revealed a heterogeneous composition of both glycosaminoglycans in terms of sulfation grade and molecular weight.
28110685	7	6	with	anion-exchange	1524:1537	arg1	detection					1564:1572	pulsed amperometric detection	1544:1572	pulsed amperometric detection	1544:1572	These peaks analyzed by high performance anion-exchange with pulsed amperometric detection and size exclusion chromatography with triple detector array, ion chromatography and by mono- or two-dimensional nuclear magnetic resonance revealed a heterogeneous composition of both glycosaminoglycans in terms of sulfation grade and molecular weight.
28110685	4	7	theme	exchange	1150:1157	arg1	chromatography					1159:1172	iv) strong anion exchange chromatography	1133:1172	iv) strong anion exchange chromatography separation	1133:1183	To pursue this aim, in this paper, for the first time, we set up a multi-analytical and preparative approach based on: i) a newly developed method by high performance anion-exchange chromatography with pulsed amperometric detection, ii) gas chromatography-mass spectrometry analyses, iii) size exclusion chromatography analyses coupled with triple detector array module and on iv) strong anion exchange chromatography separation.
28110685	3	8	theme	pharmaceutical	590:603	arg1	sulfate					622:628	pharmaceutical grade chondrotin sulfate	590:628	pharmaceutical grade chondrotin sulfate	590:628	Considering the strict regulatory rules on the pureness of pharmaceutical grade chondrotin sulfate there is an urgent need and interest to determine the residual keratan sulfate with specific, sensitive and reliable methods.
28110685	3	9	dep	need	649:652	arg1	an					639:640	an	639:640	an	639:640	Considering the strict regulatory rules on the pureness of pharmaceutical grade chondrotin sulfate there is an urgent need and interest to determine the residual keratan sulfate with specific, sensitive and reliable methods.
28110685	8	10	theme	proteoglycan	1954:1965	arg1	core					1967:1970	a proteoglycan core	1952:1970	a proteoglycan core	1952:1970	High molecular weight species (>100 KDa) were also present in the samples that counted for chains still partially linked to a proteoglycan core.
28110685	7	11	with	detection	1564:1572	arg1	array					1629:1633	triple detector array	1613:1633	triple detector array	1613:1633	These peaks analyzed by high performance anion-exchange with pulsed amperometric detection and size exclusion chromatography with triple detector array, ion chromatography and by mono- or two-dimensional nuclear magnetic resonance revealed a heterogeneous composition of both glycosaminoglycans in terms of sulfation grade and molecular weight.
28110685	1	12	theme	anti-osteoarthritis	195:213	arg1	drugs					215:219	anti-osteoarthritis drugs	195:219	anti-osteoarthritis drugs	195:219	Chondroitin sulfate is a glycosaminoglycan widely used as active principle of anti-osteoarthritis drugs and nutraceuticals, manufactured by extraction from animal cartilaginous tissues.
28110685	5	13	from	%	1238:1238	arg1	percentages					1196:1206	Varied KS percentages	1186:1206	Varied KS percentages	1186:1206	Varied KS percentages, in the range from 0.1 to 19.0% (w/w), were determined in seven pharmacopeia and commercial standards and nine commercial samples of different animal origin and manufacturers.
28110685	5	13	from	%	1238:1238	arg1	range					1216:1220	the range	1212:1220	the range from 0.1 to 19.0% (w/w)	1212:1244	Varied KS percentages, in the range from 0.1 to 19.0% (w/w), were determined in seven pharmacopeia and commercial standards and nine commercial samples of different animal origin and manufacturers.
28110685	7	14	theme	nuclear	1687:1693	arg1	resonance					1704:1712	mono- or two-dimensional nuclear magnetic resonance	1662:1712	mono- or two-dimensional nuclear magnetic resonance	1662:1712	These peaks analyzed by high performance anion-exchange with pulsed amperometric detection and size exclusion chromatography with triple detector array, ion chromatography and by mono- or two-dimensional nuclear magnetic resonance revealed a heterogeneous composition of both glycosaminoglycans in terms of sulfation grade and molecular weight.
28110685	4	15	theme	pulsed	958:963	arg1	detection					978:986	pulsed amperometric detection	958:986	pulsed amperometric detection	958:986	To pursue this aim, in this paper, for the first time, we set up a multi-analytical and preparative approach based on: i) a newly developed method by high performance anion-exchange chromatography with pulsed amperometric detection, ii) gas chromatography-mass spectrometry analyses, iii) size exclusion chromatography analyses coupled with triple detector array module and on iv) strong anion exchange chromatography separation.
28110685	4	16	theme	strong	1137:1142	arg1	chromatography					1159:1172	iv) strong anion exchange chromatography	1133:1172	iv) strong anion exchange chromatography separation	1133:1183	To pursue this aim, in this paper, for the first time, we set up a multi-analytical and preparative approach based on: i) a newly developed method by high performance anion-exchange chromatography with pulsed amperometric detection, ii) gas chromatography-mass spectrometry analyses, iii) size exclusion chromatography analyses coupled with triple detector array module and on iv) strong anion exchange chromatography separation.
28110685	4	17	from	approach	856:863	arg1	separation					1174:1183	iv) strong anion exchange chromatography separation	1133:1183	iv) strong anion exchange chromatography separation	1133:1183	To pursue this aim, in this paper, for the first time, we set up a multi-analytical and preparative approach based on: i) a newly developed method by high performance anion-exchange chromatography with pulsed amperometric detection, ii) gas chromatography-mass spectrometry analyses, iii) size exclusion chromatography analyses coupled with triple detector array module and on iv) strong anion exchange chromatography separation.
28110685	7	18	theme	weight	1820:1825	arg1	terms					1781:1785	terms	1781:1785	terms of sulfation grade and molecular weight	1781:1825	These peaks analyzed by high performance anion-exchange with pulsed amperometric detection and size exclusion chromatography with triple detector array, ion chromatography and by mono- or two-dimensional nuclear magnetic resonance revealed a heterogeneous composition of both glycosaminoglycans in terms of sulfation grade and molecular weight.
28110685	4	19	theme	performance	911:921	arg1	chromatography					938:951	high performance anion-exchange chromatography	906:951	high performance anion-exchange chromatography with pulsed amperometric detection	906:986	To pursue this aim, in this paper, for the first time, we set up a multi-analytical and preparative approach based on: i) a newly developed method by high performance anion-exchange chromatography with pulsed amperometric detection, ii) gas chromatography-mass spectrometry analyses, iii) size exclusion chromatography analyses coupled with triple detector array module and on iv) strong anion exchange chromatography separation.
28110685	5	20	dep	%	1238:1238	arg1	to					1231:1232	to	1231:1232	to	1231:1232	Varied KS percentages, in the range from 0.1 to 19.0% (w/w), were determined in seven pharmacopeia and commercial standards and nine commercial samples of different animal origin and manufacturers.
28110685	8	21	from	present	1879:1885	arg1	samples					1894:1900	the samples	1890:1900	the samples that counted for chains still partially linked to a proteoglycan core	1890:1970	High molecular weight species (>100 KDa) were also present in the samples that counted for chains still partially linked to a proteoglycan core.
28110685	3	22	theme	residual	684:691	arg1	sulfate					701:707	the residual keratan sulfate	680:707	the residual keratan sulfate with specific, sensitive and reliable methods	680:753	Considering the strict regulatory rules on the pureness of pharmaceutical grade chondrotin sulfate there is an urgent need and interest to determine the residual keratan sulfate with specific, sensitive and reliable methods.
28110685	8	23	located	present	1879:1885	arg2	>100 KDa					1859:1866	>100 KDa	1859:1866	>100 KDa	1859:1866	High molecular weight species (>100 KDa) were also present in the samples that counted for chains still partially linked to a proteoglycan core.
28110685	8	23	located	present	1879:1885	arg2	species					1850:1856	High molecular weight species	1828:1856	High molecular weight species (>100 KDa)	1828:1867	High molecular weight species (>100 KDa) were also present in the samples that counted for chains still partially linked to a proteoglycan core.
28110685	8	23	located	present	1879:1885	arg1	samples					1894:1900	the samples	1890:1900	the samples that counted for chains still partially linked to a proteoglycan core	1890:1970	High molecular weight species (>100 KDa) were also present in the samples that counted for chains still partially linked to a proteoglycan core.
28110685	4	24	theme	gas	993:995	arg1	analyses					1030:1037	ii) gas chromatography-mass spectrometry analyses	989:1037	ii) gas chromatography-mass spectrometry analyses	989:1037	To pursue this aim, in this paper, for the first time, we set up a multi-analytical and preparative approach based on: i) a newly developed method by high performance anion-exchange chromatography with pulsed amperometric detection, ii) gas chromatography-mass spectrometry analyses, iii) size exclusion chromatography analyses coupled with triple detector array module and on iv) strong anion exchange chromatography separation.
28110685	4	24	theme	gas	993:995	arg1	method					896:901	a newly developed method	878:901	: i) a newly developed method by high performance anion-exchange chromatography with pulsed amperometric detection	873:986	To pursue this aim, in this paper, for the first time, we set up a multi-analytical and preparative approach based on: i) a newly developed method by high performance anion-exchange chromatography with pulsed amperometric detection, ii) gas chromatography-mass spectrometry analyses, iii) size exclusion chromatography analyses coupled with triple detector array module and on iv) strong anion exchange chromatography separation.
28110685	6	25	theme	chromatography	1406:1419	arg1	profiles					1421:1428	Strong anion exchange chromatography profiles	1384:1428	Strong anion exchange chromatography profiles of the samples	1384:1443	Strong anion exchange chromatography profiles of the samples showed three or four different peaks.
28110685	4	26	dep	chromatography	1159:1172	arg1	iv					1133:1134	iv	1133:1134	iv	1133:1134	To pursue this aim, in this paper, for the first time, we set up a multi-analytical and preparative approach based on: i) a newly developed method by high performance anion-exchange chromatography with pulsed amperometric detection, ii) gas chromatography-mass spectrometry analyses, iii) size exclusion chromatography analyses coupled with triple detector array module and on iv) strong anion exchange chromatography separation.
28110685	7	27	theme	sulfation	1790:1798	arg1	grade					1800:1804	sulfation grade	1790:1804	sulfation grade	1790:1804	These peaks analyzed by high performance anion-exchange with pulsed amperometric detection and size exclusion chromatography with triple detector array, ion chromatography and by mono- or two-dimensional nuclear magnetic resonance revealed a heterogeneous composition of both glycosaminoglycans in terms of sulfation grade and molecular weight.
28110685	4	28	theme	spectrometry	1017:1028	arg1	analyses					1030:1037	ii) gas chromatography-mass spectrometry analyses	989:1037	ii) gas chromatography-mass spectrometry analyses	989:1037	To pursue this aim, in this paper, for the first time, we set up a multi-analytical and preparative approach based on: i) a newly developed method by high performance anion-exchange chromatography with pulsed amperometric detection, ii) gas chromatography-mass spectrometry analyses, iii) size exclusion chromatography analyses coupled with triple detector array module and on iv) strong anion exchange chromatography separation.
28110685	4	28	theme	spectrometry	1017:1028	arg1	method					896:901	a newly developed method	878:901	: i) a newly developed method by high performance anion-exchange chromatography with pulsed amperometric detection	873:986	To pursue this aim, in this paper, for the first time, we set up a multi-analytical and preparative approach based on: i) a newly developed method by high performance anion-exchange chromatography with pulsed amperometric detection, ii) gas chromatography-mass spectrometry analyses, iii) size exclusion chromatography analyses coupled with triple detector array module and on iv) strong anion exchange chromatography separation.
28110685	4	29	dep	method	896:901	arg1	i					875:875	i	875:875	i	875:875	To pursue this aim, in this paper, for the first time, we set up a multi-analytical and preparative approach based on: i) a newly developed method by high performance anion-exchange chromatography with pulsed amperometric detection, ii) gas chromatography-mass spectrometry analyses, iii) size exclusion chromatography analyses coupled with triple detector array module and on iv) strong anion exchange chromatography separation.
28110685	7	30	theme	detector	1620:1627	arg1	array					1629:1633	triple detector array	1613:1633	triple detector array	1613:1633	These peaks analyzed by high performance anion-exchange with pulsed amperometric detection and size exclusion chromatography with triple detector array, ion chromatography and by mono- or two-dimensional nuclear magnetic resonance revealed a heterogeneous composition of both glycosaminoglycans in terms of sulfation grade and molecular weight.
28110685	4	31	theme	first	799:803	arg1	time					805:808	the first time	795:808	the first time	795:808	To pursue this aim, in this paper, for the first time, we set up a multi-analytical and preparative approach based on: i) a newly developed method by high performance anion-exchange chromatography with pulsed amperometric detection, ii) gas chromatography-mass spectrometry analyses, iii) size exclusion chromatography analyses coupled with triple detector array module and on iv) strong anion exchange chromatography separation.
28110685	3	32	theme	chondrotin	611:620	arg1	sulfate					622:628	pharmaceutical grade chondrotin sulfate	590:628	pharmaceutical grade chondrotin sulfate	590:628	Considering the strict regulatory rules on the pureness of pharmaceutical grade chondrotin sulfate there is an urgent need and interest to determine the residual keratan sulfate with specific, sensitive and reliable methods.
28110685	7	33	theme	glycosaminoglycans	1759:1776	arg1	composition					1739:1749	a heterogeneous composition	1723:1749	a heterogeneous composition of both glycosaminoglycans in terms of sulfation grade and molecular weight	1723:1825	These peaks analyzed by high performance anion-exchange with pulsed amperometric detection and size exclusion chromatography with triple detector array, ion chromatography and by mono- or two-dimensional nuclear magnetic resonance revealed a heterogeneous composition of both glycosaminoglycans in terms of sulfation grade and molecular weight.
28110685	5	34	theme	different	1341:1349	arg1	origin					1358:1363	different animal origin	1341:1363	different animal origin	1341:1363	Varied KS percentages, in the range from 0.1 to 19.0% (w/w), were determined in seven pharmacopeia and commercial standards and nine commercial samples of different animal origin and manufacturers.
28110685	0	35	dep	origin	89:94	arg1	sulfate					108:114	chondroitin sulfate	96:114	animal origin chondroitin sulfate	82:114	A multi-analytical approach to better assess the keratan sulfate contamination in animal origin chondroitin sulfate.
28110685	4	36	with	chromatography	938:951	arg1	detection					978:986	pulsed amperometric detection	958:986	pulsed amperometric detection	958:986	To pursue this aim, in this paper, for the first time, we set up a multi-analytical and preparative approach based on: i) a newly developed method by high performance anion-exchange chromatography with pulsed amperometric detection, ii) gas chromatography-mass spectrometry analyses, iii) size exclusion chromatography analyses coupled with triple detector array module and on iv) strong anion exchange chromatography separation.
28110685	5	37	theme	origin	1358:1363	arg1	samples					1330:1336	nine commercial samples	1314:1336	nine commercial samples of different animal origin	1314:1363	Varied KS percentages, in the range from 0.1 to 19.0% (w/w), were determined in seven pharmacopeia and commercial standards and nine commercial samples of different animal origin and manufacturers.
28110685	5	37	theme	origin	1358:1363	arg1	manufacturers					1369:1381	manufacturers	1369:1381	manufacturers	1369:1381	Varied KS percentages, in the range from 0.1 to 19.0% (w/w), were determined in seven pharmacopeia and commercial standards and nine commercial samples of different animal origin and manufacturers.
28110685	5	37	theme	origin	1358:1363	arg1	pharmacopeia					1272:1283	seven pharmacopeia	1266:1283	seven pharmacopeia	1266:1283	Varied KS percentages, in the range from 0.1 to 19.0% (w/w), were determined in seven pharmacopeia and commercial standards and nine commercial samples of different animal origin and manufacturers.
28110685	5	37	theme	origin	1358:1363	arg1	standards					1300:1308	commercial standards	1289:1308	commercial standards	1289:1308	Varied KS percentages, in the range from 0.1 to 19.0% (w/w), were determined in seven pharmacopeia and commercial standards and nine commercial samples of different animal origin and manufacturers.
28110685	7	38	theme	heterogeneous	1725:1737	arg1	composition					1739:1749	a heterogeneous composition	1723:1749	a heterogeneous composition of both glycosaminoglycans in terms of sulfation grade and molecular weight	1723:1825	These peaks analyzed by high performance anion-exchange with pulsed amperometric detection and size exclusion chromatography with triple detector array, ion chromatography and by mono- or two-dimensional nuclear magnetic resonance revealed a heterogeneous composition of both glycosaminoglycans in terms of sulfation grade and molecular weight.
28110685	7	39	theme	size	1578:1581	arg1	chromatography					1593:1606	size exclusion chromatography	1578:1606	size exclusion chromatography	1578:1606	These peaks analyzed by high performance anion-exchange with pulsed amperometric detection and size exclusion chromatography with triple detector array, ion chromatography and by mono- or two-dimensional nuclear magnetic resonance revealed a heterogeneous composition of both glycosaminoglycans in terms of sulfation grade and molecular weight.
28110685	3	40	theme	specific	714:721	arg1	methods					747:753	specific, sensitive and reliable methods	714:753	specific, sensitive and reliable methods	714:753	Considering the strict regulatory rules on the pureness of pharmaceutical grade chondrotin sulfate there is an urgent need and interest to determine the residual keratan sulfate with specific, sensitive and reliable methods.
28110685	6	41	theme	samples	1437:1443	arg1	profiles					1421:1428	Strong anion exchange chromatography profiles	1384:1428	Strong anion exchange chromatography profiles of the samples	1384:1443	Strong anion exchange chromatography profiles of the samples showed three or four different peaks.
28110685	5	42	theme	Varied	1186:1191	arg1	percentages					1196:1206	Varied KS percentages	1186:1206	Varied KS percentages	1186:1206	Varied KS percentages, in the range from 0.1 to 19.0% (w/w), were determined in seven pharmacopeia and commercial standards and nine commercial samples of different animal origin and manufacturers.
28110685	2	43	theme	structural	508:517	arg1	similarity					519:528	its structural similarity	504:528	its structural similarity	504:528	During the manufacturing procedures, another glycosaminoglycan, the keratan sulfate, might be contemporarily withdrawn, thus eventually constituting a contaminant difficult to be determined because of its structural similarity.
28110685	0	44	theme	sulfate	57:63	arg1	contamination					65:77	the keratan sulfate contamination	45:77	the keratan sulfate contamination	45:77	A multi-analytical approach to better assess the keratan sulfate contamination in animal origin chondroitin sulfate.
28110685	3	45	theme	strict	547:552	arg1	rules					565:569	the strict regulatory rules	543:569	the strict regulatory rules on the pureness of pharmaceutical grade chondrotin sulfate	543:628	Considering the strict regulatory rules on the pureness of pharmaceutical grade chondrotin sulfate there is an urgent need and interest to determine the residual keratan sulfate with specific, sensitive and reliable methods.
28110685	7	46	theme	pulsed	1544:1549	arg1	detection					1564:1572	pulsed amperometric detection	1544:1572	pulsed amperometric detection	1544:1572	These peaks analyzed by high performance anion-exchange with pulsed amperometric detection and size exclusion chromatography with triple detector array, ion chromatography and by mono- or two-dimensional nuclear magnetic resonance revealed a heterogeneous composition of both glycosaminoglycans in terms of sulfation grade and molecular weight.
28110685	3	47	theme	reliable	738:745	arg1	methods					747:753	specific, sensitive and reliable methods	714:753	specific, sensitive and reliable methods	714:753	Considering the strict regulatory rules on the pureness of pharmaceutical grade chondrotin sulfate there is an urgent need and interest to determine the residual keratan sulfate with specific, sensitive and reliable methods.
28110685	2	48	theme	keratan	371:377	arg1	sulfate					379:385	the keratan sulfate	367:385	the keratan sulfate	367:385	During the manufacturing procedures, another glycosaminoglycan, the keratan sulfate, might be contemporarily withdrawn, thus eventually constituting a contaminant difficult to be determined because of its structural similarity.
28110685	2	48	theme	keratan	371:377	arg1	glycosaminoglycan					348:364	another glycosaminoglycan	340:364	another glycosaminoglycan	340:364	During the manufacturing procedures, another glycosaminoglycan, the keratan sulfate, might be contemporarily withdrawn, thus eventually constituting a contaminant difficult to be determined because of its structural similarity.
28110685	7	49	theme	anion-exchange	1524:1537	arg1	chromatography					1640:1653	ion chromatography	1636:1653	ion chromatography	1636:1653	These peaks analyzed by high performance anion-exchange with pulsed amperometric detection and size exclusion chromatography with triple detector array, ion chromatography and by mono- or two-dimensional nuclear magnetic resonance revealed a heterogeneous composition of both glycosaminoglycans in terms of sulfation grade and molecular weight.
28110685	7	49	theme	anion-exchange	1524:1537	arg1	performance					1512:1522	high performance	1507:1522	high performance anion-exchange with pulsed amperometric detection and size exclusion chromatography with triple detector array	1507:1633	These peaks analyzed by high performance anion-exchange with pulsed amperometric detection and size exclusion chromatography with triple detector array, ion chromatography and by mono- or two-dimensional nuclear magnetic resonance revealed a heterogeneous composition of both glycosaminoglycans in terms of sulfation grade and molecular weight.
28110685	8	50	attach	present	1879:1885	arg2	>100 KDa					1859:1866	>100 KDa	1859:1866	>100 KDa	1859:1866	High molecular weight species (>100 KDa) were also present in the samples that counted for chains still partially linked to a proteoglycan core.
28110685	8	50	attach	present	1879:1885	arg2	species					1850:1856	High molecular weight species	1828:1856	High molecular weight species (>100 KDa)	1828:1867	High molecular weight species (>100 KDa) were also present in the samples that counted for chains still partially linked to a proteoglycan core.
28110685	8	50	attach	present	1879:1885	arg1	samples					1894:1900	the samples	1890:1900	the samples that counted for chains still partially linked to a proteoglycan core	1890:1970	High molecular weight species (>100 KDa) were also present in the samples that counted for chains still partially linked to a proteoglycan core.
28110685	3	51	theme	sensitive	724:732	arg1	methods					747:753	specific, sensitive and reliable methods	714:753	specific, sensitive and reliable methods	714:753	Considering the strict regulatory rules on the pureness of pharmaceutical grade chondrotin sulfate there is an urgent need and interest to determine the residual keratan sulfate with specific, sensitive and reliable methods.
28110685	7	52	theme	high	1507:1510	arg1	chromatography					1640:1653	ion chromatography	1636:1653	ion chromatography	1636:1653	These peaks analyzed by high performance anion-exchange with pulsed amperometric detection and size exclusion chromatography with triple detector array, ion chromatography and by mono- or two-dimensional nuclear magnetic resonance revealed a heterogeneous composition of both glycosaminoglycans in terms of sulfation grade and molecular weight.
28110685	7	52	theme	high	1507:1510	arg1	performance					1512:1522	high performance	1507:1522	high performance anion-exchange with pulsed amperometric detection and size exclusion chromatography with triple detector array	1507:1633	These peaks analyzed by high performance anion-exchange with pulsed amperometric detection and size exclusion chromatography with triple detector array, ion chromatography and by mono- or two-dimensional nuclear magnetic resonance revealed a heterogeneous composition of both glycosaminoglycans in terms of sulfation grade and molecular weight.
28110685	6	53	theme	exchange	1397:1404	arg1	profiles					1421:1428	Strong anion exchange chromatography profiles	1384:1428	Strong anion exchange chromatography profiles of the samples	1384:1443	Strong anion exchange chromatography profiles of the samples showed three or four different peaks.
28110685	5	54	from	percentages	1196:1206	arg1	w/w					1241:1243	w/w	1241:1243	w/w	1241:1243	Varied KS percentages, in the range from 0.1 to 19.0% (w/w), were determined in seven pharmacopeia and commercial standards and nine commercial samples of different animal origin and manufacturers.
28110685	5	54	from	percentages	1196:1206	arg1	%					1238:1238	0.1 to 19.0%	1227:1238	0.1 to 19.0% (w/w)	1227:1244	Varied KS percentages, in the range from 0.1 to 19.0% (w/w), were determined in seven pharmacopeia and commercial standards and nine commercial samples of different animal origin and manufacturers.
28110685	5	54	from	percentages	1196:1206	arg1	range					1216:1220	the range	1212:1220	the range from 0.1 to 19.0% (w/w)	1212:1244	Varied KS percentages, in the range from 0.1 to 19.0% (w/w), were determined in seven pharmacopeia and commercial standards and nine commercial samples of different animal origin and manufacturers.
28110685	1	55	theme	animal	273:278	arg1	tissues					294:300	animal cartilaginous tissues	273:300	animal cartilaginous tissues	273:300	Chondroitin sulfate is a glycosaminoglycan widely used as active principle of anti-osteoarthritis drugs and nutraceuticals, manufactured by extraction from animal cartilaginous tissues.
28110685	4	56	theme	detector	1104:1111	arg1	module					1119:1124	triple detector array module	1097:1124	triple detector array module	1097:1124	To pursue this aim, in this paper, for the first time, we set up a multi-analytical and preparative approach based on: i) a newly developed method by high performance anion-exchange chromatography with pulsed amperometric detection, ii) gas chromatography-mass spectrometry analyses, iii) size exclusion chromatography analyses coupled with triple detector array module and on iv) strong anion exchange chromatography separation.
28110685	3	57	theme	grade	605:609	arg1	sulfate					622:628	pharmaceutical grade chondrotin sulfate	590:628	pharmaceutical grade chondrotin sulfate	590:628	Considering the strict regulatory rules on the pureness of pharmaceutical grade chondrotin sulfate there is an urgent need and interest to determine the residual keratan sulfate with specific, sensitive and reliable methods.
28110685	4	58	theme	chromatography	1159:1172	arg1	separation					1174:1183	iv) strong anion exchange chromatography separation	1133:1183	iv) strong anion exchange chromatography separation	1133:1183	To pursue this aim, in this paper, for the first time, we set up a multi-analytical and preparative approach based on: i) a newly developed method by high performance anion-exchange chromatography with pulsed amperometric detection, ii) gas chromatography-mass spectrometry analyses, iii) size exclusion chromatography analyses coupled with triple detector array module and on iv) strong anion exchange chromatography separation.
28110685	1	59	theme	active	175:180	arg1	principle					182:190	active principle	175:190	active principle of anti-osteoarthritis drugs and nutraceuticals, manufactured by extraction from animal cartilaginous tissues	175:300	Chondroitin sulfate is a glycosaminoglycan widely used as active principle of anti-osteoarthritis drugs and nutraceuticals, manufactured by extraction from animal cartilaginous tissues.
28110685	6	60	theme	anion	1391:1395	arg1	profiles					1421:1428	Strong anion exchange chromatography profiles	1384:1428	Strong anion exchange chromatography profiles of the samples	1384:1443	Strong anion exchange chromatography profiles of the samples showed three or four different peaks.
28110685	4	61	dep	exclusion	1050:1058	arg1	iii					1040:1042	iii	1040:1042	iii	1040:1042	To pursue this aim, in this paper, for the first time, we set up a multi-analytical and preparative approach based on: i) a newly developed method by high performance anion-exchange chromatography with pulsed amperometric detection, ii) gas chromatography-mass spectrometry analyses, iii) size exclusion chromatography analyses coupled with triple detector array module and on iv) strong anion exchange chromatography separation.
28110685	7	62	theme	magnetic	1695:1702	arg1	resonance					1704:1712	mono- or two-dimensional nuclear magnetic resonance	1662:1712	mono- or two-dimensional nuclear magnetic resonance	1662:1712	These peaks analyzed by high performance anion-exchange with pulsed amperometric detection and size exclusion chromatography with triple detector array, ion chromatography and by mono- or two-dimensional nuclear magnetic resonance revealed a heterogeneous composition of both glycosaminoglycans in terms of sulfation grade and molecular weight.
28110685	8	63	attach	linked	1942:1947	arg2	chains					1919:1924	chains	1919:1924	chains still partially linked to a proteoglycan core	1919:1970	High molecular weight species (>100 KDa) were also present in the samples that counted for chains still partially linked to a proteoglycan core.
28110685	8	63	attach	linked	1942:1947	arg1	core					1967:1970	a proteoglycan core	1952:1970	a proteoglycan core	1952:1970	High molecular weight species (>100 KDa) were also present in the samples that counted for chains still partially linked to a proteoglycan core.
28110685	3	64	theme	sulfate	622:628	arg1	pureness					578:585	the pureness	574:585	the pureness of pharmaceutical grade chondrotin sulfate	574:628	Considering the strict regulatory rules on the pureness of pharmaceutical grade chondrotin sulfate there is an urgent need and interest to determine the residual keratan sulfate with specific, sensitive and reliable methods.
28110685	3	65	with	sulfate	701:707	arg1	methods					747:753	specific, sensitive and reliable methods	714:753	specific, sensitive and reliable methods	714:753	Considering the strict regulatory rules on the pureness of pharmaceutical grade chondrotin sulfate there is an urgent need and interest to determine the residual keratan sulfate with specific, sensitive and reliable methods.
28110685	1	66	theme	drugs	215:219	arg1	principle					182:190	active principle	175:190	active principle of anti-osteoarthritis drugs and nutraceuticals, manufactured by extraction from animal cartilaginous tissues	175:300	Chondroitin sulfate is a glycosaminoglycan widely used as active principle of anti-osteoarthritis drugs and nutraceuticals, manufactured by extraction from animal cartilaginous tissues.
28110685	7	67	theme	two-dimensional	1671:1685	arg1	resonance					1704:1712	mono- or two-dimensional nuclear magnetic resonance	1662:1712	mono- or two-dimensional nuclear magnetic resonance	1662:1712	These peaks analyzed by high performance anion-exchange with pulsed amperometric detection and size exclusion chromatography with triple detector array, ion chromatography and by mono- or two-dimensional nuclear magnetic resonance revealed a heterogeneous composition of both glycosaminoglycans in terms of sulfation grade and molecular weight.
28110685	4	68	theme	anion-exchange	923:936	arg1	chromatography					938:951	high performance anion-exchange chromatography	906:951	high performance anion-exchange chromatography with pulsed amperometric detection	906:986	To pursue this aim, in this paper, for the first time, we set up a multi-analytical and preparative approach based on: i) a newly developed method by high performance anion-exchange chromatography with pulsed amperometric detection, ii) gas chromatography-mass spectrometry analyses, iii) size exclusion chromatography analyses coupled with triple detector array module and on iv) strong anion exchange chromatography separation.
28110685	4	69	theme	anion	1144:1148	arg1	chromatography					1159:1172	iv) strong anion exchange chromatography	1133:1172	iv) strong anion exchange chromatography separation	1133:1183	To pursue this aim, in this paper, for the first time, we set up a multi-analytical and preparative approach based on: i) a newly developed method by high performance anion-exchange chromatography with pulsed amperometric detection, ii) gas chromatography-mass spectrometry analyses, iii) size exclusion chromatography analyses coupled with triple detector array module and on iv) strong anion exchange chromatography separation.
28110685	1	70	theme	nutraceuticals	225:238	arg1	principle					182:190	active principle	175:190	active principle of anti-osteoarthritis drugs and nutraceuticals, manufactured by extraction from animal cartilaginous tissues	175:300	Chondroitin sulfate is a glycosaminoglycan widely used as active principle of anti-osteoarthritis drugs and nutraceuticals, manufactured by extraction from animal cartilaginous tissues.
28110685	7	71	theme	mono-	1662:1666	arg1	resonance					1704:1712	mono- or two-dimensional nuclear magnetic resonance	1662:1712	mono- or two-dimensional nuclear magnetic resonance	1662:1712	These peaks analyzed by high performance anion-exchange with pulsed amperometric detection and size exclusion chromatography with triple detector array, ion chromatography and by mono- or two-dimensional nuclear magnetic resonance revealed a heterogeneous composition of both glycosaminoglycans in terms of sulfation grade and molecular weight.
28110685	6	72	theme	Strong	1384:1389	arg1	profiles					1421:1428	Strong anion exchange chromatography profiles	1384:1428	Strong anion exchange chromatography profiles of the samples	1384:1443	Strong anion exchange chromatography profiles of the samples showed three or four different peaks.
28110685	3	73	theme	urgent	642:647	arg1	need					649:652	urgent need	642:652	urgent need	642:652	Considering the strict regulatory rules on the pureness of pharmaceutical grade chondrotin sulfate there is an urgent need and interest to determine the residual keratan sulfate with specific, sensitive and reliable methods.
28110685	8	74	theme	High	1828:1831	arg1	>100 KDa					1859:1866	>100 KDa	1859:1866	>100 KDa	1859:1866	High molecular weight species (>100 KDa) were also present in the samples that counted for chains still partially linked to a proteoglycan core.
28110685	8	74	theme	High	1828:1831	arg1	species					1850:1856	High molecular weight species	1828:1856	High molecular weight species (>100 KDa)	1828:1867	High molecular weight species (>100 KDa) were also present in the samples that counted for chains still partially linked to a proteoglycan core.
28110685	1	75	theme	Chondroitin	117:127	arg1	sulfate					129:135	Chondroitin sulfate	117:135	Chondroitin sulfate	117:135	Chondroitin sulfate is a glycosaminoglycan widely used as active principle of anti-osteoarthritis drugs and nutraceuticals, manufactured by extraction from animal cartilaginous tissues.
28110685	1	75	theme	Chondroitin	117:127	arg1	glycosaminoglycan					142:158	a glycosaminoglycan	140:158	a glycosaminoglycan widely used as active principle of anti-osteoarthritis drugs and nutraceuticals, manufactured by extraction from animal cartilaginous tissues	140:300	Chondroitin sulfate is a glycosaminoglycan widely used as active principle of anti-osteoarthritis drugs and nutraceuticals, manufactured by extraction from animal cartilaginous tissues.
28110685	4	76	theme	method	896:901	arg1	analyses					1075:1082	: i) a newly developed method by high performance anion-exchange chromatography with pulsed amperometric detection, ii) gas chromatography-mass spectrometry analyses, iii) size exclusion chromatography analyses	873:1082	: i) a newly developed method by high performance anion-exchange chromatography with pulsed amperometric detection, ii) gas chromatography-mass spectrometry analyses, iii) size exclusion chromatography analyses coupled with triple detector array module	873:1124	To pursue this aim, in this paper, for the first time, we set up a multi-analytical and preparative approach based on: i) a newly developed method by high performance anion-exchange chromatography with pulsed amperometric detection, ii) gas chromatography-mass spectrometry analyses, iii) size exclusion chromatography analyses coupled with triple detector array module and on iv) strong anion exchange chromatography separation.
28110685	7	77	from	composition	1739:1749	arg1	terms					1781:1785	terms	1781:1785	terms of sulfation grade and molecular weight	1781:1825	These peaks analyzed by high performance anion-exchange with pulsed amperometric detection and size exclusion chromatography with triple detector array, ion chromatography and by mono- or two-dimensional nuclear magnetic resonance revealed a heterogeneous composition of both glycosaminoglycans in terms of sulfation grade and molecular weight.
28110685	8	78	theme	weight	1843:1848	arg1	>100 KDa					1859:1866	>100 KDa	1859:1866	>100 KDa	1859:1866	High molecular weight species (>100 KDa) were also present in the samples that counted for chains still partially linked to a proteoglycan core.
28110685	8	78	theme	weight	1843:1848	arg1	species					1850:1856	High molecular weight species	1828:1856	High molecular weight species (>100 KDa)	1828:1867	High molecular weight species (>100 KDa) were also present in the samples that counted for chains still partially linked to a proteoglycan core.
28110685	7	79	theme	ion	1636:1638	arg1	performance					1512:1522	high performance	1507:1522	high performance anion-exchange with pulsed amperometric detection and size exclusion chromatography with triple detector array	1507:1633	These peaks analyzed by high performance anion-exchange with pulsed amperometric detection and size exclusion chromatography with triple detector array, ion chromatography and by mono- or two-dimensional nuclear magnetic resonance revealed a heterogeneous composition of both glycosaminoglycans in terms of sulfation grade and molecular weight.
28110685	7	79	theme	ion	1636:1638	arg1	chromatography					1640:1653	ion chromatography	1636:1653	ion chromatography	1636:1653	These peaks analyzed by high performance anion-exchange with pulsed amperometric detection and size exclusion chromatography with triple detector array, ion chromatography and by mono- or two-dimensional nuclear magnetic resonance revealed a heterogeneous composition of both glycosaminoglycans in terms of sulfation grade and molecular weight.
28110685	4	80	theme	high	906:909	arg1	chromatography					938:951	high performance anion-exchange chromatography	906:951	high performance anion-exchange chromatography with pulsed amperometric detection	906:986	To pursue this aim, in this paper, for the first time, we set up a multi-analytical and preparative approach based on: i) a newly developed method by high performance anion-exchange chromatography with pulsed amperometric detection, ii) gas chromatography-mass spectrometry analyses, iii) size exclusion chromatography analyses coupled with triple detector array module and on iv) strong anion exchange chromatography separation.
28110685	5	81	theme	commercial	1289:1298	arg1	standards					1300:1308	commercial standards	1289:1308	commercial standards	1289:1308	Varied KS percentages, in the range from 0.1 to 19.0% (w/w), were determined in seven pharmacopeia and commercial standards and nine commercial samples of different animal origin and manufacturers.
28110685	7	82	theme	molecular	1810:1818	arg1	weight					1820:1825	molecular weight	1810:1825	molecular weight	1810:1825	These peaks analyzed by high performance anion-exchange with pulsed amperometric detection and size exclusion chromatography with triple detector array, ion chromatography and by mono- or two-dimensional nuclear magnetic resonance revealed a heterogeneous composition of both glycosaminoglycans in terms of sulfation grade and molecular weight.
28110685	0	83	theme	animal	82:87	arg1	origin					89:94	animal origin chondroitin sulfate	82:114	animal origin chondroitin sulfate	82:114	A multi-analytical approach to better assess the keratan sulfate contamination in animal origin chondroitin sulfate.
28110685	7	84	theme	grade	1800:1804	arg1	terms					1781:1785	terms	1781:1785	terms of sulfation grade and molecular weight	1781:1825	These peaks analyzed by high performance anion-exchange with pulsed amperometric detection and size exclusion chromatography with triple detector array, ion chromatography and by mono- or two-dimensional nuclear magnetic resonance revealed a heterogeneous composition of both glycosaminoglycans in terms of sulfation grade and molecular weight.
28110685	7	85	theme	triple	1613:1618	arg1	array					1629:1633	triple detector array	1613:1633	triple detector array	1613:1633	These peaks analyzed by high performance anion-exchange with pulsed amperometric detection and size exclusion chromatography with triple detector array, ion chromatography and by mono- or two-dimensional nuclear magnetic resonance revealed a heterogeneous composition of both glycosaminoglycans in terms of sulfation grade and molecular weight.
28110685	4	86	theme	chromatography-mass	997:1015	arg1	analyses					1030:1037	ii) gas chromatography-mass spectrometry analyses	989:1037	ii) gas chromatography-mass spectrometry analyses	989:1037	To pursue this aim, in this paper, for the first time, we set up a multi-analytical and preparative approach based on: i) a newly developed method by high performance anion-exchange chromatography with pulsed amperometric detection, ii) gas chromatography-mass spectrometry analyses, iii) size exclusion chromatography analyses coupled with triple detector array module and on iv) strong anion exchange chromatography separation.
28110685	4	86	theme	chromatography-mass	997:1015	arg1	method					896:901	a newly developed method	878:901	: i) a newly developed method by high performance anion-exchange chromatography with pulsed amperometric detection	873:986	To pursue this aim, in this paper, for the first time, we set up a multi-analytical and preparative approach based on: i) a newly developed method by high performance anion-exchange chromatography with pulsed amperometric detection, ii) gas chromatography-mass spectrometry analyses, iii) size exclusion chromatography analyses coupled with triple detector array module and on iv) strong anion exchange chromatography separation.
28110685	8	87	from	samples	1894:1900	arg1	present					1879:1885	present	1879:1885	present	1879:1885	High molecular weight species (>100 KDa) were also present in the samples that counted for chains still partially linked to a proteoglycan core.
28110685	0	88	theme	chondroitin	96:106	arg1	sulfate					108:114	chondroitin sulfate	96:114	animal origin chondroitin sulfate	82:114	A multi-analytical approach to better assess the keratan sulfate contamination in animal origin chondroitin sulfate.
28110685	4	89	theme	amperometric	965:976	arg1	detection					978:986	pulsed amperometric detection	958:986	pulsed amperometric detection	958:986	To pursue this aim, in this paper, for the first time, we set up a multi-analytical and preparative approach based on: i) a newly developed method by high performance anion-exchange chromatography with pulsed amperometric detection, ii) gas chromatography-mass spectrometry analyses, iii) size exclusion chromatography analyses coupled with triple detector array module and on iv) strong anion exchange chromatography separation.
28110685	5	90	theme	animal	1351:1356	arg1	origin					1358:1363	different animal origin	1341:1363	different animal origin	1341:1363	Varied KS percentages, in the range from 0.1 to 19.0% (w/w), were determined in seven pharmacopeia and commercial standards and nine commercial samples of different animal origin and manufacturers.
28110685	0	91	theme	multi-analytical	2:17	arg1	approach					19:26	A multi-analytical approach	0:26	A multi-analytical approach	0:26	A multi-analytical approach to better assess the keratan sulfate contamination in animal origin chondroitin sulfate.
28110685	4	92	theme	size	1045:1048	arg1	exclusion					1050:1058	iii) size exclusion	1040:1058	iii) size exclusion	1040:1058	To pursue this aim, in this paper, for the first time, we set up a multi-analytical and preparative approach based on: i) a newly developed method by high performance anion-exchange chromatography with pulsed amperometric detection, ii) gas chromatography-mass spectrometry analyses, iii) size exclusion chromatography analyses coupled with triple detector array module and on iv) strong anion exchange chromatography separation.
28110685	4	92	theme	size	1045:1048	arg1	method					896:901	a newly developed method	878:901	: i) a newly developed method by high performance anion-exchange chromatography with pulsed amperometric detection	873:986	To pursue this aim, in this paper, for the first time, we set up a multi-analytical and preparative approach based on: i) a newly developed method by high performance anion-exchange chromatography with pulsed amperometric detection, ii) gas chromatography-mass spectrometry analyses, iii) size exclusion chromatography analyses coupled with triple detector array module and on iv) strong anion exchange chromatography separation.
28110685	4	93	theme	chromatography	1060:1073	arg1	analyses					1075:1082	: i) a newly developed method by high performance anion-exchange chromatography with pulsed amperometric detection, ii) gas chromatography-mass spectrometry analyses, iii) size exclusion chromatography analyses	873:1082	: i) a newly developed method by high performance anion-exchange chromatography with pulsed amperometric detection, ii) gas chromatography-mass spectrometry analyses, iii) size exclusion chromatography analyses coupled with triple detector array module	873:1124	To pursue this aim, in this paper, for the first time, we set up a multi-analytical and preparative approach based on: i) a newly developed method by high performance anion-exchange chromatography with pulsed amperometric detection, ii) gas chromatography-mass spectrometry analyses, iii) size exclusion chromatography analyses coupled with triple detector array module and on iv) strong anion exchange chromatography separation.
28110685	5	94	theme	commercial	1319:1328	arg1	samples					1330:1336	nine commercial samples	1314:1336	nine commercial samples of different animal origin	1314:1363	Varied KS percentages, in the range from 0.1 to 19.0% (w/w), were determined in seven pharmacopeia and commercial standards and nine commercial samples of different animal origin and manufacturers.
28110685	2	95	theme	difficult	466:474	arg1	contaminant					454:464	a contaminant	452:464	a contaminant difficult to be determined because of its structural similarity	452:528	During the manufacturing procedures, another glycosaminoglycan, the keratan sulfate, might be contemporarily withdrawn, thus eventually constituting a contaminant difficult to be determined because of its structural similarity.
28110685	7	96	theme	exclusion	1583:1591	arg1	chromatography					1593:1606	size exclusion chromatography	1578:1606	size exclusion chromatography	1578:1606	These peaks analyzed by high performance anion-exchange with pulsed amperometric detection and size exclusion chromatography with triple detector array, ion chromatography and by mono- or two-dimensional nuclear magnetic resonance revealed a heterogeneous composition of both glycosaminoglycans in terms of sulfation grade and molecular weight.
28110685	3	97	theme	keratan	693:699	arg1	sulfate					701:707	the residual keratan sulfate	680:707	the residual keratan sulfate with specific, sensitive and reliable methods	680:753	Considering the strict regulatory rules on the pureness of pharmaceutical grade chondrotin sulfate there is an urgent need and interest to determine the residual keratan sulfate with specific, sensitive and reliable methods.
28110685	4	98	theme	multi-analytical	823:838	arg1	approach					856:863	a multi-analytical and preparative approach	821:863	a multi-analytical and preparative approach based on: i) a newly developed method by high performance anion-exchange chromatography with pulsed amperometric detection, ii) gas chromatography-mass spectrometry analyses, iii) size exclusion chromatography analyses coupled with triple detector array module and on iv) strong anion exchange chromatography separation	821:1183	To pursue this aim, in this paper, for the first time, we set up a multi-analytical and preparative approach based on: i) a newly developed method by high performance anion-exchange chromatography with pulsed amperometric detection, ii) gas chromatography-mass spectrometry analyses, iii) size exclusion chromatography analyses coupled with triple detector array module and on iv) strong anion exchange chromatography separation.
28110685	0	99	theme	keratan	49:55	arg1	sulfate					57:63	the keratan sulfate	45:63	the keratan sulfate contamination	45:77	A multi-analytical approach to better assess the keratan sulfate contamination in animal origin chondroitin sulfate.
28110685	5	100	theme	KS	1193:1194	arg1	percentages					1196:1206	Varied KS percentages	1186:1206	Varied KS percentages	1186:1206	Varied KS percentages, in the range from 0.1 to 19.0% (w/w), were determined in seven pharmacopeia and commercial standards and nine commercial samples of different animal origin and manufacturers.
28110685	4	101	theme	preparative	844:854	arg1	approach					856:863	a multi-analytical and preparative approach	821:863	a multi-analytical and preparative approach based on: i) a newly developed method by high performance anion-exchange chromatography with pulsed amperometric detection, ii) gas chromatography-mass spectrometry analyses, iii) size exclusion chromatography analyses coupled with triple detector array module and on iv) strong anion exchange chromatography separation	821:1183	To pursue this aim, in this paper, for the first time, we set up a multi-analytical and preparative approach based on: i) a newly developed method by high performance anion-exchange chromatography with pulsed amperometric detection, ii) gas chromatography-mass spectrometry analyses, iii) size exclusion chromatography analyses coupled with triple detector array module and on iv) strong anion exchange chromatography separation.
28110685	3	102	theme	regulatory	554:563	arg1	rules					565:569	the strict regulatory rules	543:569	the strict regulatory rules on the pureness of pharmaceutical grade chondrotin sulfate	543:628	Considering the strict regulatory rules on the pureness of pharmaceutical grade chondrotin sulfate there is an urgent need and interest to determine the residual keratan sulfate with specific, sensitive and reliable methods.
28110685	8	103	theme	molecular	1833:1841	arg1	>100 KDa					1859:1866	>100 KDa	1859:1866	>100 KDa	1859:1866	High molecular weight species (>100 KDa) were also present in the samples that counted for chains still partially linked to a proteoglycan core.
28110685	8	103	theme	molecular	1833:1841	arg1	species					1850:1856	High molecular weight species	1828:1856	High molecular weight species (>100 KDa)	1828:1867	High molecular weight species (>100 KDa) were also present in the samples that counted for chains still partially linked to a proteoglycan core.
28110685	7	104	theme	amperometric	1551:1562	arg1	detection					1564:1572	pulsed amperometric detection	1544:1572	pulsed amperometric detection	1544:1572	These peaks analyzed by high performance anion-exchange with pulsed amperometric detection and size exclusion chromatography with triple detector array, ion chromatography and by mono- or two-dimensional nuclear magnetic resonance revealed a heterogeneous composition of both glycosaminoglycans in terms of sulfation grade and molecular weight.
28110685	4	105	theme	array	1113:1117	arg1	module					1119:1124	triple detector array module	1097:1124	triple detector array module	1097:1124	To pursue this aim, in this paper, for the first time, we set up a multi-analytical and preparative approach based on: i) a newly developed method by high performance anion-exchange chromatography with pulsed amperometric detection, ii) gas chromatography-mass spectrometry analyses, iii) size exclusion chromatography analyses coupled with triple detector array module and on iv) strong anion exchange chromatography separation.
28110685	7	106	with	chromatography	1593:1606	arg1	array					1629:1633	triple detector array	1613:1633	triple detector array	1613:1633	These peaks analyzed by high performance anion-exchange with pulsed amperometric detection and size exclusion chromatography with triple detector array, ion chromatography and by mono- or two-dimensional nuclear magnetic resonance revealed a heterogeneous composition of both glycosaminoglycans in terms of sulfation grade and molecular weight.
28110685	6	107	theme	different	1466:1474	arg1	peaks					1476:1480	three or four different peaks	1452:1480	three or four different peaks	1452:1480	Strong anion exchange chromatography profiles of the samples showed three or four different peaks.
27929093	7	0	theme	plant-based	1462:1472	arg1	diets					1474:1478	plant-based diets	1462:1478	plant-based diets	1462:1478	These results show for the first time that specific monosaccharides found in the cell wall polysaccharides in plant-based diets can act as active ingredients that reduce CHD by inhibiting postocclusion steps, including MI and heart failure.
27929093	1	1	theme	cohort	171:176	arg1	studies					178:184	Many cohort studies	166:184	Many cohort studies	166:184	Many cohort studies have shown that consumption of diets containing a higher composition of foods derived from plants reduces mortality from coronary heart disease (CHD).
27929093	2	2	theme	rat	469:471	arg1	models					473:478	three rat models	463:478	three rat models	463:478	Here, we examined the active components of a plant-based diet and the underlying mechanisms that reduce the risk of CHD using three rat models and a quantitative proteomics approach.
27929093	5	3	theme	tail-vein	1133:1141	arg1	injections					1143:1152	tail-vein injections	1133:1152	tail-vein injections	1133:1152	Among the wheat components, arabinose and xylose were identified as active components responsible for the observed efficacy of WE, which was administered via ingestion and tail-vein injections.
27929093	6	4	from	arabinose	1254:1262	arg1	rich					1246:1249	rich	1246:1249	rich	1246:1249	Finally, the food components of plant-based diets that contained cell wall polysaccharides rich in arabinose, xylose, and possibly fucose were found to confer protection against myocardial injury.
27929093	0	5	theme	myocardial	111:120	arg1	injury					122:127	myocardial injury	111:127	myocardial injury in rat myocardial infarction models	111:163	Plant-based foods containing cell wall polysaccharides rich in specific active monosaccharides protect against myocardial injury in rat myocardial infarction models.
27929093	1	6	from	disease	322:328	arg1	mortality					292:300	mortality	292:300	mortality from coronary heart disease (CHD)	292:334	Many cohort studies have shown that consumption of diets containing a higher composition of foods derived from plants reduces mortality from coronary heart disease (CHD).
27929093	3	7	theme	short-term	525:534	arg1	MI					559:560	MI	559:560	MI	559:560	In a short-term myocardial infarction (MI) model, intake of wheat extract (WE), the representative cardioprotectant identified by screening approximately 4,000 samples, reduced myocardial injury by inhibiting apoptosis, enhancing ATP production, and maintaining protein homeostasis.
27929093	3	7	theme	short-term	525:534	arg1	infarction					547:556	a short-term myocardial infarction	523:556	a short-term myocardial infarction (MI) model	523:567	In a short-term myocardial infarction (MI) model, intake of wheat extract (WE), the representative cardioprotectant identified by screening approximately 4,000 samples, reduced myocardial injury by inhibiting apoptosis, enhancing ATP production, and maintaining protein homeostasis.
27929093	7	8	from	polysaccharides	1443:1457	arg1	diets					1474:1478	plant-based diets	1462:1478	plant-based diets	1462:1478	These results show for the first time that specific monosaccharides found in the cell wall polysaccharides in plant-based diets can act as active ingredients that reduce CHD by inhibiting postocclusion steps, including MI and heart failure.
27929093	4	9	theme	heart	946:950	arg1	failure					952:958	heart failure	946:958	heart failure	946:958	In long-term post-MI models, this myocardial protection resulted in ameliorating adverse left-ventricular remodelling, which is a predictor of heart failure.
27929093	5	10	theme	wheat	971:975	arg1	components					977:986	the wheat components	967:986	the wheat components	967:986	Among the wheat components, arabinose and xylose were identified as active components responsible for the observed efficacy of WE, which was administered via ingestion and tail-vein injections.
27929093	3	11	theme	myocardial	536:545	arg1	MI					559:560	MI	559:560	MI	559:560	In a short-term myocardial infarction (MI) model, intake of wheat extract (WE), the representative cardioprotectant identified by screening approximately 4,000 samples, reduced myocardial injury by inhibiting apoptosis, enhancing ATP production, and maintaining protein homeostasis.
27929093	3	11	theme	myocardial	536:545	arg1	infarction					547:556	a short-term myocardial infarction	523:556	a short-term myocardial infarction (MI) model	523:567	In a short-term myocardial infarction (MI) model, intake of wheat extract (WE), the representative cardioprotectant identified by screening approximately 4,000 samples, reduced myocardial injury by inhibiting apoptosis, enhancing ATP production, and maintaining protein homeostasis.
27929093	7	12	theme	heart	1578:1582	arg1	failure					1584:1590	heart failure	1578:1590	heart failure	1578:1590	These results show for the first time that specific monosaccharides found in the cell wall polysaccharides in plant-based diets can act as active ingredients that reduce CHD by inhibiting postocclusion steps, including MI and heart failure.
27929093	6	13	from	xylose	1265:1270	arg1	rich					1246:1249	rich	1246:1249	rich	1246:1249	Finally, the food components of plant-based diets that contained cell wall polysaccharides rich in arabinose, xylose, and possibly fucose were found to confer protection against myocardial injury.
27929093	2	14	theme	proteomics	499:508	arg1	approach					510:517	a quantitative proteomics approach	484:517	a quantitative proteomics approach	484:517	Here, we examined the active components of a plant-based diet and the underlying mechanisms that reduce the risk of CHD using three rat models and a quantitative proteomics approach.
27929093	0	15	from	rich	55:58	arg1	monosaccharides					79:93	specific active monosaccharides	63:93	specific active monosaccharides	63:93	Plant-based foods containing cell wall polysaccharides rich in specific active monosaccharides protect against myocardial injury in rat myocardial infarction models.
27929093	2	16	theme	underlying	407:416	arg1	mechanisms					418:427	the underlying mechanisms	403:427	the underlying mechanisms that reduce the risk of CHD	403:455	Here, we examined the active components of a plant-based diet and the underlying mechanisms that reduce the risk of CHD using three rat models and a quantitative proteomics approach.
27929093	2	17	theme	diet	394:397	arg1	mechanisms					418:427	the underlying mechanisms	403:427	the underlying mechanisms that reduce the risk of CHD	403:455	Here, we examined the active components of a plant-based diet and the underlying mechanisms that reduce the risk of CHD using three rat models and a quantitative proteomics approach.
27929093	2	17	theme	diet	394:397	arg1	components					366:375	the active components	355:375	the active components of a plant-based diet	355:397	Here, we examined the active components of a plant-based diet and the underlying mechanisms that reduce the risk of CHD using three rat models and a quantitative proteomics approach.
27929093	2	18	theme	quantitative	486:497	arg1	approach					510:517	a quantitative proteomics approach	484:517	a quantitative proteomics approach	484:517	Here, we examined the active components of a plant-based diet and the underlying mechanisms that reduce the risk of CHD using three rat models and a quantitative proteomics approach.
27929093	0	19	contain	containing	18:27	arg2	polysaccharides					39:53	cell wall polysaccharides	29:53	cell wall polysaccharides rich in specific active monosaccharides	29:93	Plant-based foods containing cell wall polysaccharides rich in specific active monosaccharides protect against myocardial injury in rat myocardial infarction models.
27929093	0	19	contain	containing	18:27	arg1	foods					12:16	Plant-based foods	0:16	Plant-based foods containing cell wall polysaccharides rich in specific active monosaccharides	0:93	Plant-based foods containing cell wall polysaccharides rich in specific active monosaccharides protect against myocardial injury in rat myocardial infarction models.
27929093	6	20	theme	food	1168:1171	arg1	components					1173:1182	the food components	1164:1182	the food components of plant-based diets that contained cell wall polysaccharides rich in arabinose, xylose, and possibly fucose	1164:1291	Finally, the food components of plant-based diets that contained cell wall polysaccharides rich in arabinose, xylose, and possibly fucose were found to confer protection against myocardial injury.
27929093	2	21	theme	plant-based	382:392	arg1	diet					394:397	a plant-based diet	380:397	a plant-based diet	380:397	Here, we examined the active components of a plant-based diet and the underlying mechanisms that reduce the risk of CHD using three rat models and a quantitative proteomics approach.
27929093	3	22	theme	myocardial	697:706	arg1	injury					708:713	myocardial injury	697:713	myocardial injury	697:713	In a short-term myocardial infarction (MI) model, intake of wheat extract (WE), the representative cardioprotectant identified by screening approximately 4,000 samples, reduced myocardial injury by inhibiting apoptosis, enhancing ATP production, and maintaining protein homeostasis.
27929093	6	23	contain	contained	1210:1218	arg1	components					1173:1182	the food components	1164:1182	the food components of plant-based diets that contained cell wall polysaccharides rich in arabinose, xylose, and possibly fucose	1164:1291	Finally, the food components of plant-based diets that contained cell wall polysaccharides rich in arabinose, xylose, and possibly fucose were found to confer protection against myocardial injury.
27929093	6	23	contain	contained	1210:1218	arg2	polysaccharides					1230:1244	cell wall polysaccharides	1220:1244	cell wall polysaccharides rich in arabinose, xylose, and possibly fucose	1220:1291	Finally, the food components of plant-based diets that contained cell wall polysaccharides rich in arabinose, xylose, and possibly fucose were found to confer protection against myocardial injury.
27929093	0	24	theme	Plant-based	0:10	arg1	foods					12:16	Plant-based foods	0:16	Plant-based foods containing cell wall polysaccharides rich in specific active monosaccharides	0:93	Plant-based foods containing cell wall polysaccharides rich in specific active monosaccharides protect against myocardial injury in rat myocardial infarction models.
27929093	7	25	theme	wall	1438:1441	arg1	polysaccharides					1443:1457	the cell wall polysaccharides	1429:1457	the cell wall polysaccharides in plant-based diets	1429:1478	These results show for the first time that specific monosaccharides found in the cell wall polysaccharides in plant-based diets can act as active ingredients that reduce CHD by inhibiting postocclusion steps, including MI and heart failure.
27929093	4	26	theme	myocardial	837:846	arg1	protection					848:857	this myocardial protection	832:857	this myocardial protection	832:857	In long-term post-MI models, this myocardial protection resulted in ameliorating adverse left-ventricular remodelling, which is a predictor of heart failure.
27929093	6	27	from	rich	1246:1249	arg1	xylose					1265:1270	xylose	1265:1270	xylose	1265:1270	Finally, the food components of plant-based diets that contained cell wall polysaccharides rich in arabinose, xylose, and possibly fucose were found to confer protection against myocardial injury.
27929093	6	27	from	rich	1246:1249	arg1	arabinose					1254:1262	arabinose	1254:1262	arabinose	1254:1262	Finally, the food components of plant-based diets that contained cell wall polysaccharides rich in arabinose, xylose, and possibly fucose were found to confer protection against myocardial injury.
27929093	6	27	from	rich	1246:1249	arg1	fucose					1286:1291	fucose	1286:1291	fucose	1286:1291	Finally, the food components of plant-based diets that contained cell wall polysaccharides rich in arabinose, xylose, and possibly fucose were found to confer protection against myocardial injury.
27929093	3	28	theme	representative	604:617	arg1	extract					586:592	wheat extract	580:592	wheat extract (WE)	580:597	In a short-term myocardial infarction (MI) model, intake of wheat extract (WE), the representative cardioprotectant identified by screening approximately 4,000 samples, reduced myocardial injury by inhibiting apoptosis, enhancing ATP production, and maintaining protein homeostasis.
27929093	3	28	theme	representative	604:617	arg1	cardioprotectant					619:634	the representative cardioprotectant	600:634	the representative cardioprotectant identified by screening approximately 4,000 samples	600:686	In a short-term myocardial infarction (MI) model, intake of wheat extract (WE), the representative cardioprotectant identified by screening approximately 4,000 samples, reduced myocardial injury by inhibiting apoptosis, enhancing ATP production, and maintaining protein homeostasis.
27929093	0	29	theme	cell	29:32	arg1	polysaccharides					39:53	cell wall polysaccharides	29:53	cell wall polysaccharides rich in specific active monosaccharides	29:93	Plant-based foods containing cell wall polysaccharides rich in specific active monosaccharides protect against myocardial injury in rat myocardial infarction models.
27929093	7	30	located	found	1420:1424	arg2	ingredients					1498:1508	active ingredients	1491:1508	active ingredients that reduce CHD by inhibiting postocclusion steps, including MI and heart failure	1491:1590	These results show for the first time that specific monosaccharides found in the cell wall polysaccharides in plant-based diets can act as active ingredients that reduce CHD by inhibiting postocclusion steps, including MI and heart failure.
27929093	7	30	located	found	1420:1424	arg2	monosaccharides					1404:1418	specific monosaccharides	1395:1418	specific monosaccharides found in the cell wall polysaccharides in plant-based diets	1395:1478	These results show for the first time that specific monosaccharides found in the cell wall polysaccharides in plant-based diets can act as active ingredients that reduce CHD by inhibiting postocclusion steps, including MI and heart failure.
27929093	7	30	located	found	1420:1424	arg1	polysaccharides					1443:1457	the cell wall polysaccharides	1429:1457	the cell wall polysaccharides in plant-based diets	1429:1478	These results show for the first time that specific monosaccharides found in the cell wall polysaccharides in plant-based diets can act as active ingredients that reduce CHD by inhibiting postocclusion steps, including MI and heart failure.
27929093	0	31	theme	myocardial	136:145	arg1	infarction					147:156	rat myocardial infarction	132:156	rat myocardial infarction models	132:163	Plant-based foods containing cell wall polysaccharides rich in specific active monosaccharides protect against myocardial injury in rat myocardial infarction models.
27929093	4	32	theme	post-MI	816:822	arg1	models					824:829	long-term post-MI models	806:829	long-term post-MI models	806:829	In long-term post-MI models, this myocardial protection resulted in ameliorating adverse left-ventricular remodelling, which is a predictor of heart failure.
27929093	3	33	theme	infarction	547:556	arg1	model					563:567	a short-term myocardial infarction (MI) model	523:567	a short-term myocardial infarction (MI) model	523:567	In a short-term myocardial infarction (MI) model, intake of wheat extract (WE), the representative cardioprotectant identified by screening approximately 4,000 samples, reduced myocardial injury by inhibiting apoptosis, enhancing ATP production, and maintaining protein homeostasis.
27929093	3	34	dep	extract	586:592	arg1	WE					595:596	WE	595:596	WE	595:596	In a short-term myocardial infarction (MI) model, intake of wheat extract (WE), the representative cardioprotectant identified by screening approximately 4,000 samples, reduced myocardial injury by inhibiting apoptosis, enhancing ATP production, and maintaining protein homeostasis.
27929093	1	35	theme	coronary	307:314	arg1	CHD					331:333	CHD	331:333	CHD	331:333	Many cohort studies have shown that consumption of diets containing a higher composition of foods derived from plants reduces mortality from coronary heart disease (CHD).
27929093	1	35	theme	coronary	307:314	arg1	disease					322:328	coronary heart disease	307:328	coronary heart disease (CHD)	307:334	Many cohort studies have shown that consumption of diets containing a higher composition of foods derived from plants reduces mortality from coronary heart disease (CHD).
27929093	0	36	theme	rat	132:134	arg1	infarction					147:156	rat myocardial infarction	132:156	rat myocardial infarction models	132:163	Plant-based foods containing cell wall polysaccharides rich in specific active monosaccharides protect against myocardial injury in rat myocardial infarction models.
27929093	6	37	theme	plant-based	1187:1197	arg1	diets					1199:1203	plant-based diets	1187:1203	plant-based diets	1187:1203	Finally, the food components of plant-based diets that contained cell wall polysaccharides rich in arabinose, xylose, and possibly fucose were found to confer protection against myocardial injury.
27929093	1	38	theme	diets	217:221	arg1	consumption					202:212	consumption	202:212	consumption of diets containing a higher composition of foods derived from plants	202:282	Many cohort studies have shown that consumption of diets containing a higher composition of foods derived from plants reduces mortality from coronary heart disease (CHD).
27929093	1	39	theme	heart	316:320	arg1	CHD					331:333	CHD	331:333	CHD	331:333	Many cohort studies have shown that consumption of diets containing a higher composition of foods derived from plants reduces mortality from coronary heart disease (CHD).
27929093	1	39	theme	heart	316:320	arg1	disease					322:328	coronary heart disease	307:328	coronary heart disease (CHD)	307:334	Many cohort studies have shown that consumption of diets containing a higher composition of foods derived from plants reduces mortality from coronary heart disease (CHD).
27929093	6	40	theme	myocardial	1333:1342	arg1	injury					1344:1349	myocardial injury	1333:1349	myocardial injury	1333:1349	Finally, the food components of plant-based diets that contained cell wall polysaccharides rich in arabinose, xylose, and possibly fucose were found to confer protection against myocardial injury.
27929093	0	41	from	monosaccharides	79:93	arg1	rich					55:58	rich	55:58	rich	55:58	Plant-based foods containing cell wall polysaccharides rich in specific active monosaccharides protect against myocardial injury in rat myocardial infarction models.
27929093	4	42	theme	long-term	806:814	arg1	models					824:829	long-term post-MI models	806:829	long-term post-MI models	806:829	In long-term post-MI models, this myocardial protection resulted in ameliorating adverse left-ventricular remodelling, which is a predictor of heart failure.
27929093	6	43	theme	diets	1199:1203	arg1	components					1173:1182	the food components	1164:1182	the food components of plant-based diets that contained cell wall polysaccharides rich in arabinose, xylose, and possibly fucose	1164:1291	Finally, the food components of plant-based diets that contained cell wall polysaccharides rich in arabinose, xylose, and possibly fucose were found to confer protection against myocardial injury.
27929093	0	44	theme	wall	34:37	arg1	polysaccharides					39:53	cell wall polysaccharides	29:53	cell wall polysaccharides rich in specific active monosaccharides	29:93	Plant-based foods containing cell wall polysaccharides rich in specific active monosaccharides protect against myocardial injury in rat myocardial infarction models.
27929093	3	45	dep	reduced	689:695	arg1	maintaining					770:780	maintaining	770:780	maintaining protein homeostasis	770:800	In a short-term myocardial infarction (MI) model, intake of wheat extract (WE), the representative cardioprotectant identified by screening approximately 4,000 samples, reduced myocardial injury by inhibiting apoptosis, enhancing ATP production, and maintaining protein homeostasis.
27929093	3	45	dep	reduced	689:695	arg1	enhancing					740:748	enhancing	740:748	enhancing ATP production	740:763	In a short-term myocardial infarction (MI) model, intake of wheat extract (WE), the representative cardioprotectant identified by screening approximately 4,000 samples, reduced myocardial injury by inhibiting apoptosis, enhancing ATP production, and maintaining protein homeostasis.
27929093	0	46	theme	infarction	147:156	arg1	models					158:163	rat myocardial infarction models	132:163	rat myocardial infarction models	132:163	Plant-based foods containing cell wall polysaccharides rich in specific active monosaccharides protect against myocardial injury in rat myocardial infarction models.
27929093	5	47	theme	WE	1088:1089	arg1	efficacy					1076:1083	the observed efficacy	1063:1083	the observed efficacy	1063:1083	Among the wheat components, arabinose and xylose were identified as active components responsible for the observed efficacy of WE, which was administered via ingestion and tail-vein injections.
27929093	6	48	theme	rich	1246:1249	arg1	polysaccharides					1230:1244	cell wall polysaccharides	1220:1244	cell wall polysaccharides rich in arabinose, xylose, and possibly fucose	1220:1291	Finally, the food components of plant-based diets that contained cell wall polysaccharides rich in arabinose, xylose, and possibly fucose were found to confer protection against myocardial injury.
27929093	7	49	theme	first	1379:1383	arg1	time					1385:1388	the first time	1375:1388	the first time	1375:1388	These results show for the first time that specific monosaccharides found in the cell wall polysaccharides in plant-based diets can act as active ingredients that reduce CHD by inhibiting postocclusion steps, including MI and heart failure.
27929093	2	50	theme	CHD	453:455	arg1	risk					445:448	the risk	441:448	the risk of CHD	441:455	Here, we examined the active components of a plant-based diet and the underlying mechanisms that reduce the risk of CHD using three rat models and a quantitative proteomics approach.
27929093	4	51	theme	failure	952:958	arg1	predictor					933:941	a predictor	931:941	a predictor of heart failure	931:958	In long-term post-MI models, this myocardial protection resulted in ameliorating adverse left-ventricular remodelling, which is a predictor of heart failure.
27929093	4	51	theme	failure	952:958	arg1	remodelling					909:919	adverse left-ventricular remodelling	884:919	adverse left-ventricular remodelling	884:919	In long-term post-MI models, this myocardial protection resulted in ameliorating adverse left-ventricular remodelling, which is a predictor of heart failure.
27929093	7	52	theme	postocclusion	1540:1552	arg1	steps					1554:1558	postocclusion steps	1540:1558	postocclusion steps	1540:1558	These results show for the first time that specific monosaccharides found in the cell wall polysaccharides in plant-based diets can act as active ingredients that reduce CHD by inhibiting postocclusion steps, including MI and heart failure.
27929093	7	52	theme	postocclusion	1540:1552	arg1	MI					1571:1572	MI	1571:1572	MI	1571:1572	These results show for the first time that specific monosaccharides found in the cell wall polysaccharides in plant-based diets can act as active ingredients that reduce CHD by inhibiting postocclusion steps, including MI and heart failure.
27929093	7	52	theme	postocclusion	1540:1552	arg1	failure					1584:1590	heart failure	1578:1590	heart failure	1578:1590	These results show for the first time that specific monosaccharides found in the cell wall polysaccharides in plant-based diets can act as active ingredients that reduce CHD by inhibiting postocclusion steps, including MI and heart failure.
27929093	5	53	theme	active	1029:1034	arg1	xylose					1003:1008	xylose	1003:1008	xylose	1003:1008	Among the wheat components, arabinose and xylose were identified as active components responsible for the observed efficacy of WE, which was administered via ingestion and tail-vein injections.
27929093	5	53	theme	active	1029:1034	arg1	arabinose					989:997	arabinose	989:997	arabinose	989:997	Among the wheat components, arabinose and xylose were identified as active components responsible for the observed efficacy of WE, which was administered via ingestion and tail-vein injections.
27929093	5	53	theme	active	1029:1034	arg1	components					1036:1045	active components	1029:1045	active components responsible for the observed efficacy of WE, which was administered via ingestion and tail-vein injections	1029:1152	Among the wheat components, arabinose and xylose were identified as active components responsible for the observed efficacy of WE, which was administered via ingestion and tail-vein injections.
27929093	0	54	theme	rich	55:58	arg1	polysaccharides					39:53	cell wall polysaccharides	29:53	cell wall polysaccharides rich in specific active monosaccharides	29:93	Plant-based foods containing cell wall polysaccharides rich in specific active monosaccharides protect against myocardial injury in rat myocardial infarction models.
27929093	3	55	theme	ATP	750:752	arg1	production					754:763	ATP production	750:763	ATP production	750:763	In a short-term myocardial infarction (MI) model, intake of wheat extract (WE), the representative cardioprotectant identified by screening approximately 4,000 samples, reduced myocardial injury by inhibiting apoptosis, enhancing ATP production, and maintaining protein homeostasis.
27929093	4	56	theme	left-ventricular	892:907	arg1	predictor					933:941	a predictor	931:941	a predictor of heart failure	931:958	In long-term post-MI models, this myocardial protection resulted in ameliorating adverse left-ventricular remodelling, which is a predictor of heart failure.
27929093	4	56	theme	left-ventricular	892:907	arg1	remodelling					909:919	adverse left-ventricular remodelling	884:919	adverse left-ventricular remodelling	884:919	In long-term post-MI models, this myocardial protection resulted in ameliorating adverse left-ventricular remodelling, which is a predictor of heart failure.
27929093	5	57	theme	responsible	1047:1057	arg1	xylose					1003:1008	xylose	1003:1008	xylose	1003:1008	Among the wheat components, arabinose and xylose were identified as active components responsible for the observed efficacy of WE, which was administered via ingestion and tail-vein injections.
27929093	5	57	theme	responsible	1047:1057	arg1	arabinose					989:997	arabinose	989:997	arabinose	989:997	Among the wheat components, arabinose and xylose were identified as active components responsible for the observed efficacy of WE, which was administered via ingestion and tail-vein injections.
27929093	5	57	theme	responsible	1047:1057	arg1	components					1036:1045	active components	1029:1045	active components responsible for the observed efficacy of WE, which was administered via ingestion and tail-vein injections	1029:1152	Among the wheat components, arabinose and xylose were identified as active components responsible for the observed efficacy of WE, which was administered via ingestion and tail-vein injections.
27929093	1	58	contain	containing	223:232	arg1	diets					217:221	diets	217:221	diets containing a higher composition of foods derived from plants	217:282	Many cohort studies have shown that consumption of diets containing a higher composition of foods derived from plants reduces mortality from coronary heart disease (CHD).
27929093	1	58	contain	containing	223:232	arg2	composition					243:253	a higher composition	234:253	a higher composition of foods derived from plants	234:282	Many cohort studies have shown that consumption of diets containing a higher composition of foods derived from plants reduces mortality from coronary heart disease (CHD).
27929093	4	59	theme	adverse	884:890	arg1	predictor					933:941	a predictor	931:941	a predictor of heart failure	931:958	In long-term post-MI models, this myocardial protection resulted in ameliorating adverse left-ventricular remodelling, which is a predictor of heart failure.
27929093	4	59	theme	adverse	884:890	arg1	remodelling					909:919	adverse left-ventricular remodelling	884:919	adverse left-ventricular remodelling	884:919	In long-term post-MI models, this myocardial protection resulted in ameliorating adverse left-ventricular remodelling, which is a predictor of heart failure.
27929093	6	60	theme	wall	1225:1228	arg1	polysaccharides					1230:1244	cell wall polysaccharides	1220:1244	cell wall polysaccharides rich in arabinose, xylose, and possibly fucose	1220:1291	Finally, the food components of plant-based diets that contained cell wall polysaccharides rich in arabinose, xylose, and possibly fucose were found to confer protection against myocardial injury.
27929093	3	61	theme	extract	586:592	arg1	intake					570:575	intake	570:575	intake of wheat extract (WE), the representative cardioprotectant identified by screening approximately 4,000 samples,	570:687	In a short-term myocardial infarction (MI) model, intake of wheat extract (WE), the representative cardioprotectant identified by screening approximately 4,000 samples, reduced myocardial injury by inhibiting apoptosis, enhancing ATP production, and maintaining protein homeostasis.
27929093	0	62	from	injury	122:127	arg1	models					158:163	rat myocardial infarction models	132:163	rat myocardial infarction models	132:163	Plant-based foods containing cell wall polysaccharides rich in specific active monosaccharides protect against myocardial injury in rat myocardial infarction models.
27929093	6	63	theme	cell	1220:1223	arg1	polysaccharides					1230:1244	cell wall polysaccharides	1220:1244	cell wall polysaccharides rich in arabinose, xylose, and possibly fucose	1220:1291	Finally, the food components of plant-based diets that contained cell wall polysaccharides rich in arabinose, xylose, and possibly fucose were found to confer protection against myocardial injury.
27929093	7	64	theme	specific	1395:1402	arg1	monosaccharides					1404:1418	specific monosaccharides	1395:1418	specific monosaccharides found in the cell wall polysaccharides in plant-based diets	1395:1478	These results show for the first time that specific monosaccharides found in the cell wall polysaccharides in plant-based diets can act as active ingredients that reduce CHD by inhibiting postocclusion steps, including MI and heart failure.
27929093	7	64	theme	specific	1395:1402	arg1	ingredients					1498:1508	active ingredients	1491:1508	active ingredients that reduce CHD by inhibiting postocclusion steps, including MI and heart failure	1491:1590	These results show for the first time that specific monosaccharides found in the cell wall polysaccharides in plant-based diets can act as active ingredients that reduce CHD by inhibiting postocclusion steps, including MI and heart failure.
27929093	1	65	theme	higher	236:241	arg1	composition					243:253	a higher composition	234:253	a higher composition of foods derived from plants	234:282	Many cohort studies have shown that consumption of diets containing a higher composition of foods derived from plants reduces mortality from coronary heart disease (CHD).
27929093	7	66	theme	cell	1433:1436	arg1	polysaccharides					1443:1457	the cell wall polysaccharides	1429:1457	the cell wall polysaccharides in plant-based diets	1429:1478	These results show for the first time that specific monosaccharides found in the cell wall polysaccharides in plant-based diets can act as active ingredients that reduce CHD by inhibiting postocclusion steps, including MI and heart failure.
27929093	1	67	attach	derived	264:270	arg1	plants					277:282	plants	277:282	plants	277:282	Many cohort studies have shown that consumption of diets containing a higher composition of foods derived from plants reduces mortality from coronary heart disease (CHD).
27929093	1	67	attach	derived	264:270	arg2	foods					258:262	foods	258:262	foods derived from plants	258:282	Many cohort studies have shown that consumption of diets containing a higher composition of foods derived from plants reduces mortality from coronary heart disease (CHD).
27929093	2	68	theme	active	359:364	arg1	components					366:375	the active components	355:375	the active components of a plant-based diet	355:397	Here, we examined the active components of a plant-based diet and the underlying mechanisms that reduce the risk of CHD using three rat models and a quantitative proteomics approach.
27929093	0	69	theme	active	72:77	arg1	monosaccharides					79:93	specific active monosaccharides	63:93	specific active monosaccharides	63:93	Plant-based foods containing cell wall polysaccharides rich in specific active monosaccharides protect against myocardial injury in rat myocardial infarction models.
27929093	5	70	theme	observed	1067:1074	arg1	efficacy					1076:1083	the observed efficacy	1063:1083	the observed efficacy	1063:1083	Among the wheat components, arabinose and xylose were identified as active components responsible for the observed efficacy of WE, which was administered via ingestion and tail-vein injections.
27929093	3	71	theme	wheat	580:584	arg1	extract					586:592	wheat extract	580:592	wheat extract (WE)	580:597	In a short-term myocardial infarction (MI) model, intake of wheat extract (WE), the representative cardioprotectant identified by screening approximately 4,000 samples, reduced myocardial injury by inhibiting apoptosis, enhancing ATP production, and maintaining protein homeostasis.
27929093	3	71	theme	wheat	580:584	arg1	cardioprotectant					619:634	the representative cardioprotectant	600:634	the representative cardioprotectant identified by screening approximately 4,000 samples	600:686	In a short-term myocardial infarction (MI) model, intake of wheat extract (WE), the representative cardioprotectant identified by screening approximately 4,000 samples, reduced myocardial injury by inhibiting apoptosis, enhancing ATP production, and maintaining protein homeostasis.
27929093	6	72	from	fucose	1286:1291	arg1	rich					1246:1249	rich	1246:1249	rich	1246:1249	Finally, the food components of plant-based diets that contained cell wall polysaccharides rich in arabinose, xylose, and possibly fucose were found to confer protection against myocardial injury.
27929093	0	73	theme	specific	63:70	arg1	monosaccharides					79:93	specific active monosaccharides	63:93	specific active monosaccharides	63:93	Plant-based foods containing cell wall polysaccharides rich in specific active monosaccharides protect against myocardial injury in rat myocardial infarction models.
27929093	7	74	theme	active	1491:1496	arg1	monosaccharides					1404:1418	specific monosaccharides	1395:1418	specific monosaccharides found in the cell wall polysaccharides in plant-based diets	1395:1478	These results show for the first time that specific monosaccharides found in the cell wall polysaccharides in plant-based diets can act as active ingredients that reduce CHD by inhibiting postocclusion steps, including MI and heart failure.
27929093	7	74	theme	active	1491:1496	arg1	ingredients					1498:1508	active ingredients	1491:1508	active ingredients that reduce CHD by inhibiting postocclusion steps, including MI and heart failure	1491:1590	These results show for the first time that specific monosaccharides found in the cell wall polysaccharides in plant-based diets can act as active ingredients that reduce CHD by inhibiting postocclusion steps, including MI and heart failure.
27929093	3	75	theme	protein	782:788	arg1	homeostasis					790:800	protein homeostasis	782:800	protein homeostasis	782:800	In a short-term myocardial infarction (MI) model, intake of wheat extract (WE), the representative cardioprotectant identified by screening approximately 4,000 samples, reduced myocardial injury by inhibiting apoptosis, enhancing ATP production, and maintaining protein homeostasis.
27929093	1	76	theme	Many	166:169	arg1	studies					178:184	Many cohort studies	166:184	Many cohort studies	166:184	Many cohort studies have shown that consumption of diets containing a higher composition of foods derived from plants reduces mortality from coronary heart disease (CHD).
27929093	1	77	theme	foods	258:262	arg1	composition					243:253	a higher composition	234:253	a higher composition of foods derived from plants	234:282	Many cohort studies have shown that consumption of diets containing a higher composition of foods derived from plants reduces mortality from coronary heart disease (CHD).
28013276	1	0	theme	G	333:333	arg1	precursors					309:318	the precursors	305:318	the precursors of guaiacyl (G) and syringyl (S) lignin subunits	305:367	Caffeoyl coenzyme A 3-O-methyltransferase (CCoAOMT) and caffeic acid-O-methyltransferase (COMT) are key enzymes in the biosynthesis of coniferyl and sinapyl alcohols, the precursors of guaiacyl (G) and syringyl (S) lignin subunits.
28013276	9	1	theme	comt	1517:1520	arg1	mutants					1522:1528	ccoaomt1 comt mutants	1508:1528	ccoaomt1 comt mutants	1508:1528	This suggests that the positive effect of lignin reduction on cell wall degradability of comt and ccoaomt1 comt mutants is counteracted by changes occurring in lignin composition, such as the decreased S/G ratio.
28013276	6	2	theme	mutant	985:990	arg1	plants					992:997	ccoaomt1 mutant plants	976:997	ccoaomt1 mutant plants	976:997	The phenotype of ccoaomt1 mutant plants displays no alterations, and their lignin content and composition remain unchanged.
28013276	3	3	theme	key	654:656	arg1	COMT					644:647	COMT	644:647	COMT	644:647	In this work, we determined that the comt (brown-midrib 3) mutant plants display a reduction of the flavonolignin unit derived from tricin (a dimethylated flavone), demonstrating that COMT is a key enzyme involved in the synthesis of this compound.
28013276	3	3	theme	key	654:656	arg1	enzyme					658:663	a key enzyme	652:663	a key enzyme involved in the synthesis of this compound	652:706	In this work, we determined that the comt (brown-midrib 3) mutant plants display a reduction of the flavonolignin unit derived from tricin (a dimethylated flavone), demonstrating that COMT is a key enzyme involved in the synthesis of this compound.
28013276	8	4	theme	cell	1324:1327	arg1	degradability					1334:1346	cell wall degradability	1324:1346	cell wall degradability	1324:1346	Although stems from the three mutants display a similar increase of hemicelluloses, the effect on cell wall degradability varies, the cell walls of ccoaomt1 being the most degradable.
28013276	9	5	theme	mutants	1522:1528	arg1	degradability					1482:1494	cell wall degradability	1472:1494	cell wall degradability of comt and ccoaomt1 comt mutants	1472:1528	This suggests that the positive effect of lignin reduction on cell wall degradability of comt and ccoaomt1 comt mutants is counteracted by changes occurring in lignin composition, such as the decreased S/G ratio.
28013276	6	6	theme	plants	992:997	arg1	phenotype					963:971	The phenotype	959:971	The phenotype of ccoaomt1 mutant plants	959:997	The phenotype of ccoaomt1 mutant plants displays no alterations, and their lignin content and composition remain unchanged.
28013276	6	7	theme	ccoaomt1	976:983	arg1	plants					992:997	ccoaomt1 mutant plants	976:997	ccoaomt1 mutant plants	976:997	The phenotype of ccoaomt1 mutant plants displays no alterations, and their lignin content and composition remain unchanged.
28013276	8	8	from	mutants	1256:1262	arg1	stems					1235:1239	stems	1235:1239	stems from the three mutants	1235:1262	Although stems from the three mutants display a similar increase of hemicelluloses, the effect on cell wall degradability varies, the cell walls of ccoaomt1 being the most degradable.
28013276	1	9	dep	enzymes	242:248	arg1	precursors					309:318	the precursors	305:318	the precursors of guaiacyl (G) and syringyl (S) lignin subunits	305:367	Caffeoyl coenzyme A 3-O-methyltransferase (CCoAOMT) and caffeic acid-O-methyltransferase (COMT) are key enzymes in the biosynthesis of coniferyl and sinapyl alcohols, the precursors of guaiacyl (G) and syringyl (S) lignin subunits.
28013276	7	10	theme	other	1090:1094	arg1	hand					1096:1099	the other hand	1086:1099	the other hand	1086:1099	On the other hand, the ccoaomt1 comt mutant displays phenotypic and lignin alterations similar to those already described for the comt mutant.
28013276	0	11	from	Changes	0:6	arg1	Wall					16:19	Cell Wall Polymers and Degradability	11:46	Wall	16:19	Changes in Cell Wall Polymers and Degradability in Maize Mutants Lacking 3'- and 5'-O-Methyltransferases Involved in Lignin Biosynthesis.
28013276	0	11	from	Changes	0:6	arg1	Degradability					34:46	Degradability	34:46	Degradability	34:46	Changes in Cell Wall Polymers and Degradability in Maize Mutants Lacking 3'- and 5'-O-Methyltransferases Involved in Lignin Biosynthesis.
28013276	0	11	from	Changes	0:6	arg1	Mutants					57:63	Maize Mutants	51:63	Maize Mutants Lacking 3'- and 5'-O-Methyltransferases	51:103	Changes in Cell Wall Polymers and Degradability in Maize Mutants Lacking 3'- and 5'-O-Methyltransferases Involved in Lignin Biosynthesis.
28013276	8	12	theme	hemicelluloses	1294:1307	arg1	increase					1282:1289	a similar increase	1272:1289	a similar increase of hemicelluloses	1272:1307	Although stems from the three mutants display a similar increase of hemicelluloses, the effect on cell wall degradability varies, the cell walls of ccoaomt1 being the most degradable.
28013276	3	13	theme	comt	497:500	arg1	plants					526:531	the comt (brown-midrib 3) mutant plants	493:531	the comt (brown-midrib 3) mutant plants	493:531	In this work, we determined that the comt (brown-midrib 3) mutant plants display a reduction of the flavonolignin unit derived from tricin (a dimethylated flavone), demonstrating that COMT is a key enzyme involved in the synthesis of this compound.
28013276	10	14	from	role	1640:1643	arg1	degradability					1704:1716	cell wall degradability	1694:1716	cell wall degradability	1694:1716	In addition, the role of the flavonolignin unit derived from tricin in cell wall degradability is also discussed.
28013276	4	15	theme	compound	849:856	arg1	synthesis					831:839	the synthesis	827:839	the synthesis of this compound	827:856	In contrast, the ccoaomt1 mutants display a wild-type amount of tricin, suggesting that CCoAOMT1 is not essential for the synthesis of this compound.
28013276	1	16	theme	syringyl	340:347	arg1	subunits					360:367	guaiacyl (G) and syringyl (S) lignin subunits	323:367	subunits	360:367	Caffeoyl coenzyme A 3-O-methyltransferase (CCoAOMT) and caffeic acid-O-methyltransferase (COMT) are key enzymes in the biosynthesis of coniferyl and sinapyl alcohols, the precursors of guaiacyl (G) and syringyl (S) lignin subunits.
28013276	1	17	theme	coniferyl	273:281	arg1	alcohols					295:302	coniferyl and sinapyl alcohols	273:302	coniferyl and sinapyl alcohols	273:302	Caffeoyl coenzyme A 3-O-methyltransferase (CCoAOMT) and caffeic acid-O-methyltransferase (COMT) are key enzymes in the biosynthesis of coniferyl and sinapyl alcohols, the precursors of guaiacyl (G) and syringyl (S) lignin subunits.
28013276	10	18	theme	wall	1699:1702	arg1	degradability					1704:1716	cell wall degradability	1694:1716	cell wall degradability	1694:1716	In addition, the role of the flavonolignin unit derived from tricin in cell wall degradability is also discussed.
28013276	10	19	theme	unit	1666:1669	arg1	role					1640:1643	the role	1636:1643	the role of the flavonolignin unit derived from tricin in cell wall degradability	1636:1716	In addition, the role of the flavonolignin unit derived from tricin in cell wall degradability is also discussed.
28013276	1	20	theme	S	350:350	arg1	subunits					360:367	guaiacyl (G) and syringyl (S) lignin subunits	323:367	subunits	360:367	Caffeoyl coenzyme A 3-O-methyltransferase (CCoAOMT) and caffeic acid-O-methyltransferase (COMT) are key enzymes in the biosynthesis of coniferyl and sinapyl alcohols, the precursors of guaiacyl (G) and syringyl (S) lignin subunits.
28013276	4	21	theme	wild-type	753:761	arg1	tricin					773:778	tricin	773:778	tricin	773:778	In contrast, the ccoaomt1 mutants display a wild-type amount of tricin, suggesting that CCoAOMT1 is not essential for the synthesis of this compound.
28013276	4	21	theme	wild-type	753:761	arg1	amount					763:768	a wild-type amount	751:768	a wild-type amount of tricin	751:778	In contrast, the ccoaomt1 mutants display a wild-type amount of tricin, suggesting that CCoAOMT1 is not essential for the synthesis of this compound.
28013276	9	22	theme	positive	1433:1440	arg1	effect					1442:1447	the positive effect	1429:1447	the positive effect of lignin reduction on cell wall degradability of comt and ccoaomt1 comt mutants	1429:1528	This suggests that the positive effect of lignin reduction on cell wall degradability of comt and ccoaomt1 comt mutants is counteracted by changes occurring in lignin composition, such as the decreased S/G ratio.
28013276	2	23	theme	single	421:426	arg1	plants					452:457	single and double mutant maize plants	421:457	single and double mutant maize plants	421:457	The function of these enzymes was characterized in single and double mutant maize plants.
28013276	4	24	theme	tricin	773:778	arg1	tricin					773:778	tricin	773:778	tricin	773:778	In contrast, the ccoaomt1 mutants display a wild-type amount of tricin, suggesting that CCoAOMT1 is not essential for the synthesis of this compound.
28013276	4	24	theme	tricin	773:778	arg1	amount					763:768	a wild-type amount	751:768	a wild-type amount of tricin	751:778	In contrast, the ccoaomt1 mutants display a wild-type amount of tricin, suggesting that CCoAOMT1 is not essential for the synthesis of this compound.
28013276	10	25	theme	flavonolignin	1652:1664	arg1	unit					1666:1669	the flavonolignin unit	1648:1669	the flavonolignin unit derived from tricin	1648:1689	In addition, the role of the flavonolignin unit derived from tricin in cell wall degradability is also discussed.
28013276	8	26	theme	cell	1360:1363	arg1	degradable					1398:1407	degradable	1398:1407	degradable	1398:1407	Although stems from the three mutants display a similar increase of hemicelluloses, the effect on cell wall degradability varies, the cell walls of ccoaomt1 being the most degradable.
28013276	8	26	theme	cell	1360:1363	arg1	walls					1365:1369	the cell walls	1356:1369	the cell walls of ccoaomt1	1356:1381	Although stems from the three mutants display a similar increase of hemicelluloses, the effect on cell wall degradability varies, the cell walls of ccoaomt1 being the most degradable.
28013276	3	27	theme	mutant	519:524	arg1	plants					526:531	the comt (brown-midrib 3) mutant plants	493:531	the comt (brown-midrib 3) mutant plants	493:531	In this work, we determined that the comt (brown-midrib 3) mutant plants display a reduction of the flavonolignin unit derived from tricin (a dimethylated flavone), demonstrating that COMT is a key enzyme involved in the synthesis of this compound.
28013276	1	28	theme	caffeic	194:200	arg1	enzymes					242:248	key enzymes	238:248	key enzymes	238:248	Caffeoyl coenzyme A 3-O-methyltransferase (CCoAOMT) and caffeic acid-O-methyltransferase (COMT) are key enzymes in the biosynthesis of coniferyl and sinapyl alcohols, the precursors of guaiacyl (G) and syringyl (S) lignin subunits.
28013276	1	28	theme	caffeic	194:200	arg1	3-O-methyltransferase					158:178	Caffeoyl coenzyme A 3-O-methyltransferase	138:178	Caffeoyl coenzyme A 3-O-methyltransferase (CCoAOMT)	138:188	Caffeoyl coenzyme A 3-O-methyltransferase (CCoAOMT) and caffeic acid-O-methyltransferase (COMT) are key enzymes in the biosynthesis of coniferyl and sinapyl alcohols, the precursors of guaiacyl (G) and syringyl (S) lignin subunits.
28013276	1	28	theme	caffeic	194:200	arg1	COMT					228:231	COMT	228:231	COMT	228:231	Caffeoyl coenzyme A 3-O-methyltransferase (CCoAOMT) and caffeic acid-O-methyltransferase (COMT) are key enzymes in the biosynthesis of coniferyl and sinapyl alcohols, the precursors of guaiacyl (G) and syringyl (S) lignin subunits.
28013276	1	28	theme	caffeic	194:200	arg1	acid-O-methyltransferase					202:225	caffeic acid-O-methyltransferase	194:225	caffeic acid-O-methyltransferase (COMT)	194:232	Caffeoyl coenzyme A 3-O-methyltransferase (CCoAOMT) and caffeic acid-O-methyltransferase (COMT) are key enzymes in the biosynthesis of coniferyl and sinapyl alcohols, the precursors of guaiacyl (G) and syringyl (S) lignin subunits.
28013276	1	29	theme	sinapyl	287:293	arg1	alcohols					295:302	coniferyl and sinapyl alcohols	273:302	coniferyl and sinapyl alcohols	273:302	Caffeoyl coenzyme A 3-O-methyltransferase (CCoAOMT) and caffeic acid-O-methyltransferase (COMT) are key enzymes in the biosynthesis of coniferyl and sinapyl alcohols, the precursors of guaiacyl (G) and syringyl (S) lignin subunits.
28013276	9	30	theme	lignin	1570:1575	arg1	composition					1577:1587	lignin composition	1570:1587	lignin composition	1570:1587	This suggests that the positive effect of lignin reduction on cell wall degradability of comt and ccoaomt1 comt mutants is counteracted by changes occurring in lignin composition, such as the decreased S/G ratio.
28013276	3	31	theme	brown-midrib	503:514	arg1	plants					526:531	the comt (brown-midrib 3) mutant plants	493:531	the comt (brown-midrib 3) mutant plants	493:531	In this work, we determined that the comt (brown-midrib 3) mutant plants display a reduction of the flavonolignin unit derived from tricin (a dimethylated flavone), demonstrating that COMT is a key enzyme involved in the synthesis of this compound.
28013276	8	32	from	effect	1314:1319	arg1	degradability					1334:1346	cell wall degradability	1324:1346	cell wall degradability	1324:1346	Although stems from the three mutants display a similar increase of hemicelluloses, the effect on cell wall degradability varies, the cell walls of ccoaomt1 being the most degradable.
28013276	2	33	theme	enzymes	392:398	arg1	function					374:381	The function	370:381	The function of these enzymes	370:398	The function of these enzymes was characterized in single and double mutant maize plants.
28013276	0	34	theme	Cell	11:14	arg1	Wall					16:19	Cell Wall Polymers and Degradability	11:46	Wall	16:19	Changes in Cell Wall Polymers and Degradability in Maize Mutants Lacking 3'- and 5'-O-Methyltransferases Involved in Lignin Biosynthesis.
28013276	1	35	theme	alcohols	295:302	arg1	biosynthesis					257:268	the biosynthesis	253:268	the biosynthesis of coniferyl and sinapyl alcohols	253:302	Caffeoyl coenzyme A 3-O-methyltransferase (CCoAOMT) and caffeic acid-O-methyltransferase (COMT) are key enzymes in the biosynthesis of coniferyl and sinapyl alcohols, the precursors of guaiacyl (G) and syringyl (S) lignin subunits.
28013276	0	36	theme	Lignin	117:122	arg1	Biosynthesis					124:135	Lignin Biosynthesis	117:135	Lignin Biosynthesis	117:135	Changes in Cell Wall Polymers and Degradability in Maize Mutants Lacking 3'- and 5'-O-Methyltransferases Involved in Lignin Biosynthesis.
28013276	1	37	theme	lignin	353:358	arg1	subunits					360:367	guaiacyl (G) and syringyl (S) lignin subunits	323:367	subunits	360:367	Caffeoyl coenzyme A 3-O-methyltransferase (CCoAOMT) and caffeic acid-O-methyltransferase (COMT) are key enzymes in the biosynthesis of coniferyl and sinapyl alcohols, the precursors of guaiacyl (G) and syringyl (S) lignin subunits.
28013276	9	38	theme	reduction	1459:1467	arg1	effect					1442:1447	the positive effect	1429:1447	the positive effect of lignin reduction on cell wall degradability of comt and ccoaomt1 comt mutants	1429:1528	This suggests that the positive effect of lignin reduction on cell wall degradability of comt and ccoaomt1 comt mutants is counteracted by changes occurring in lignin composition, such as the decreased S/G ratio.
28013276	7	39	theme	phenotypic	1136:1145	arg1	alterations					1158:1168	phenotypic and lignin alterations	1136:1168	alterations	1158:1168	On the other hand, the ccoaomt1 comt mutant displays phenotypic and lignin alterations similar to those already described for the comt mutant.
28013276	2	40	theme	maize	446:450	arg1	plants					452:457	single and double mutant maize plants	421:457	single and double mutant maize plants	421:457	The function of these enzymes was characterized in single and double mutant maize plants.
28013276	1	41	theme	subunits	360:367	arg1	precursors					309:318	the precursors	305:318	the precursors of guaiacyl (G) and syringyl (S) lignin subunits	305:367	Caffeoyl coenzyme A 3-O-methyltransferase (CCoAOMT) and caffeic acid-O-methyltransferase (COMT) are key enzymes in the biosynthesis of coniferyl and sinapyl alcohols, the precursors of guaiacyl (G) and syringyl (S) lignin subunits.
28013276	9	42	theme	cell	1472:1475	arg1	degradability					1482:1494	cell wall degradability	1472:1494	cell wall degradability of comt and ccoaomt1 comt mutants	1472:1528	This suggests that the positive effect of lignin reduction on cell wall degradability of comt and ccoaomt1 comt mutants is counteracted by changes occurring in lignin composition, such as the decreased S/G ratio.
28013276	7	43	theme	comt	1213:1216	arg1	mutant					1218:1223	the comt mutant	1209:1223	the comt mutant	1209:1223	On the other hand, the ccoaomt1 comt mutant displays phenotypic and lignin alterations similar to those already described for the comt mutant.
28013276	2	44	theme	mutant	439:444	arg1	plants					452:457	single and double mutant maize plants	421:457	single and double mutant maize plants	421:457	The function of these enzymes was characterized in single and double mutant maize plants.
28013276	3	45	theme	compound	699:706	arg1	synthesis					681:689	the synthesis	677:689	the synthesis of this compound	677:706	In this work, we determined that the comt (brown-midrib 3) mutant plants display a reduction of the flavonolignin unit derived from tricin (a dimethylated flavone), demonstrating that COMT is a key enzyme involved in the synthesis of this compound.
28013276	9	46	theme	comt	1499:1502	arg1	degradability					1482:1494	cell wall degradability	1472:1494	cell wall degradability of comt and ccoaomt1 comt mutants	1472:1528	This suggests that the positive effect of lignin reduction on cell wall degradability of comt and ccoaomt1 comt mutants is counteracted by changes occurring in lignin composition, such as the decreased S/G ratio.
28013276	4	47	theme	ccoaomt1	726:733	arg1	mutants					735:741	the ccoaomt1 mutants	722:741	the ccoaomt1 mutants	722:741	In contrast, the ccoaomt1 mutants display a wild-type amount of tricin, suggesting that CCoAOMT1 is not essential for the synthesis of this compound.
28013276	9	48	theme	lignin	1452:1457	arg1	reduction					1459:1467	lignin reduction	1452:1467	lignin reduction	1452:1467	This suggests that the positive effect of lignin reduction on cell wall degradability of comt and ccoaomt1 comt mutants is counteracted by changes occurring in lignin composition, such as the decreased S/G ratio.
28013276	2	49	theme	double	432:437	arg1	plants					452:457	single and double mutant maize plants	421:457	single and double mutant maize plants	421:457	The function of these enzymes was characterized in single and double mutant maize plants.
28013276	8	50	dep	varies	1348:1353	arg1	degradable					1398:1407	degradable	1398:1407	degradable	1398:1407	Although stems from the three mutants display a similar increase of hemicelluloses, the effect on cell wall degradability varies, the cell walls of ccoaomt1 being the most degradable.
28013276	8	50	dep	varies	1348:1353	arg1	walls					1365:1369	the cell walls	1356:1369	the cell walls of ccoaomt1	1356:1381	Although stems from the three mutants display a similar increase of hemicelluloses, the effect on cell wall degradability varies, the cell walls of ccoaomt1 being the most degradable.
28013276	1	51	from	enzymes	242:248	arg1	biosynthesis					257:268	the biosynthesis	253:268	the biosynthesis of coniferyl and sinapyl alcohols	253:302	Caffeoyl coenzyme A 3-O-methyltransferase (CCoAOMT) and caffeic acid-O-methyltransferase (COMT) are key enzymes in the biosynthesis of coniferyl and sinapyl alcohols, the precursors of guaiacyl (G) and syringyl (S) lignin subunits.
28013276	7	52	theme	comt	1115:1118	arg1	mutant					1120:1125	the ccoaomt1 comt mutant	1102:1125	the ccoaomt1 comt mutant	1102:1125	On the other hand, the ccoaomt1 comt mutant displays phenotypic and lignin alterations similar to those already described for the comt mutant.
28013276	5	53	theme	lignin	918:923	arg1	biosynthesis					925:936	lignin biosynthesis	918:936	lignin biosynthesis	918:936	Based on our data, we suggest that CCoAOMT1 is involved in lignin biosynthesis at least in midribs.
28013276	9	54	theme	decreased	1602:1610	arg1	ratio					1616:1620	the decreased S/G ratio	1598:1620	the decreased S/G ratio	1598:1620	This suggests that the positive effect of lignin reduction on cell wall degradability of comt and ccoaomt1 comt mutants is counteracted by changes occurring in lignin composition, such as the decreased S/G ratio.
28013276	7	55	theme	similar	1170:1176	arg1	alterations					1158:1168	phenotypic and lignin alterations	1136:1168	alterations	1158:1168	On the other hand, the ccoaomt1 comt mutant displays phenotypic and lignin alterations similar to those already described for the comt mutant.
28013276	3	56	attach	derived	579:585	arg2	unit					574:577	the flavonolignin unit	556:577	the flavonolignin unit derived from tricin (a dimethylated flavone)	556:622	In this work, we determined that the comt (brown-midrib 3) mutant plants display a reduction of the flavonolignin unit derived from tricin (a dimethylated flavone), demonstrating that COMT is a key enzyme involved in the synthesis of this compound.
28013276	3	56	attach	derived	579:585	arg1	tricin					592:597	tricin	592:597	tricin (a dimethylated flavone)	592:622	In this work, we determined that the comt (brown-midrib 3) mutant plants display a reduction of the flavonolignin unit derived from tricin (a dimethylated flavone), demonstrating that COMT is a key enzyme involved in the synthesis of this compound.
28013276	3	56	attach	derived	579:585	arg1	flavone					615:621	a dimethylated flavone	600:621	a dimethylated flavone	600:621	In this work, we determined that the comt (brown-midrib 3) mutant plants display a reduction of the flavonolignin unit derived from tricin (a dimethylated flavone), demonstrating that COMT is a key enzyme involved in the synthesis of this compound.
28013276	10	57	theme	cell	1694:1697	arg1	degradability					1704:1716	cell wall degradability	1694:1716	cell wall degradability	1694:1716	In addition, the role of the flavonolignin unit derived from tricin in cell wall degradability is also discussed.
28013276	10	58	attach	derived	1671:1677	arg1	tricin					1684:1689	tricin	1684:1689	tricin	1684:1689	In addition, the role of the flavonolignin unit derived from tricin in cell wall degradability is also discussed.
28013276	10	58	attach	derived	1671:1677	arg2	unit					1666:1669	the flavonolignin unit	1648:1669	the flavonolignin unit derived from tricin	1648:1689	In addition, the role of the flavonolignin unit derived from tricin in cell wall degradability is also discussed.
28013276	1	59	theme	guaiacyl	323:330	arg1	G					333:333	guaiacyl (G) and syringyl (S) lignin subunits	323:367	G	333:333	Caffeoyl coenzyme A 3-O-methyltransferase (CCoAOMT) and caffeic acid-O-methyltransferase (COMT) are key enzymes in the biosynthesis of coniferyl and sinapyl alcohols, the precursors of guaiacyl (G) and syringyl (S) lignin subunits.
28013276	9	60	theme	wall	1477:1480	arg1	degradability					1482:1494	cell wall degradability	1472:1494	cell wall degradability of comt and ccoaomt1 comt mutants	1472:1528	This suggests that the positive effect of lignin reduction on cell wall degradability of comt and ccoaomt1 comt mutants is counteracted by changes occurring in lignin composition, such as the decreased S/G ratio.
28013276	3	61	theme	flavonolignin	560:572	arg1	unit					574:577	the flavonolignin unit	556:577	the flavonolignin unit derived from tricin (a dimethylated flavone)	556:622	In this work, we determined that the comt (brown-midrib 3) mutant plants display a reduction of the flavonolignin unit derived from tricin (a dimethylated flavone), demonstrating that COMT is a key enzyme involved in the synthesis of this compound.
28013276	0	62	dep	Wall	16:19	arg1	Polymers					21:28	Polymers	21:28	Polymers	21:28	Changes in Cell Wall Polymers and Degradability in Maize Mutants Lacking 3'- and 5'-O-Methyltransferases Involved in Lignin Biosynthesis.
28013276	8	63	theme	similar	1274:1280	arg1	increase					1282:1289	a similar increase	1272:1289	a similar increase of hemicelluloses	1272:1307	Although stems from the three mutants display a similar increase of hemicelluloses, the effect on cell wall degradability varies, the cell walls of ccoaomt1 being the most degradable.
28013276	7	64	theme	lignin	1151:1156	arg1	alterations					1158:1168	phenotypic and lignin alterations	1136:1168	alterations	1158:1168	On the other hand, the ccoaomt1 comt mutant displays phenotypic and lignin alterations similar to those already described for the comt mutant.
28013276	6	65	theme	lignin	1034:1039	arg1	content					1041:1047	their lignin content	1028:1047	their lignin content	1028:1047	The phenotype of ccoaomt1 mutant plants displays no alterations, and their lignin content and composition remain unchanged.
28013276	9	66	theme	S/G	1612:1614	arg1	ratio					1616:1620	the decreased S/G ratio	1598:1620	the decreased S/G ratio	1598:1620	This suggests that the positive effect of lignin reduction on cell wall degradability of comt and ccoaomt1 comt mutants is counteracted by changes occurring in lignin composition, such as the decreased S/G ratio.
28013276	8	67	theme	wall	1329:1332	arg1	degradability					1334:1346	cell wall degradability	1324:1346	cell wall degradability	1324:1346	Although stems from the three mutants display a similar increase of hemicelluloses, the effect on cell wall degradability varies, the cell walls of ccoaomt1 being the most degradable.
28013276	3	68	theme	unit	574:577	arg1	reduction					543:551	a reduction	541:551	a reduction of the flavonolignin unit derived from tricin (a dimethylated flavone)	541:622	In this work, we determined that the comt (brown-midrib 3) mutant plants display a reduction of the flavonolignin unit derived from tricin (a dimethylated flavone), demonstrating that COMT is a key enzyme involved in the synthesis of this compound.
28013276	1	69	theme	Caffeoyl	138:145	arg1	A					156:156	Caffeoyl coenzyme A	138:156	Caffeoyl coenzyme A 3-O-methyltransferase (CCoAOMT)	138:188	Caffeoyl coenzyme A 3-O-methyltransferase (CCoAOMT) and caffeic acid-O-methyltransferase (COMT) are key enzymes in the biosynthesis of coniferyl and sinapyl alcohols, the precursors of guaiacyl (G) and syringyl (S) lignin subunits.
28013276	9	70	from	effect	1442:1447	arg1	degradability					1482:1494	cell wall degradability	1472:1494	cell wall degradability of comt and ccoaomt1 comt mutants	1472:1528	This suggests that the positive effect of lignin reduction on cell wall degradability of comt and ccoaomt1 comt mutants is counteracted by changes occurring in lignin composition, such as the decreased S/G ratio.
28013276	1	71	theme	key	238:240	arg1	enzymes					242:248	key enzymes	238:248	key enzymes	238:248	Caffeoyl coenzyme A 3-O-methyltransferase (CCoAOMT) and caffeic acid-O-methyltransferase (COMT) are key enzymes in the biosynthesis of coniferyl and sinapyl alcohols, the precursors of guaiacyl (G) and syringyl (S) lignin subunits.
28013276	1	71	theme	key	238:240	arg1	3-O-methyltransferase					158:178	Caffeoyl coenzyme A 3-O-methyltransferase	138:178	Caffeoyl coenzyme A 3-O-methyltransferase (CCoAOMT)	138:188	Caffeoyl coenzyme A 3-O-methyltransferase (CCoAOMT) and caffeic acid-O-methyltransferase (COMT) are key enzymes in the biosynthesis of coniferyl and sinapyl alcohols, the precursors of guaiacyl (G) and syringyl (S) lignin subunits.
28013276	1	71	theme	key	238:240	arg1	acid-O-methyltransferase					202:225	caffeic acid-O-methyltransferase	194:225	caffeic acid-O-methyltransferase (COMT)	194:232	Caffeoyl coenzyme A 3-O-methyltransferase (CCoAOMT) and caffeic acid-O-methyltransferase (COMT) are key enzymes in the biosynthesis of coniferyl and sinapyl alcohols, the precursors of guaiacyl (G) and syringyl (S) lignin subunits.
28013276	7	72	theme	ccoaomt1	1106:1113	arg1	mutant					1120:1125	the ccoaomt1 comt mutant	1102:1125	the ccoaomt1 comt mutant	1102:1125	On the other hand, the ccoaomt1 comt mutant displays phenotypic and lignin alterations similar to those already described for the comt mutant.
28013276	3	73	theme	dimethylated	602:613	arg1	tricin					592:597	tricin	592:597	tricin (a dimethylated flavone)	592:622	In this work, we determined that the comt (brown-midrib 3) mutant plants display a reduction of the flavonolignin unit derived from tricin (a dimethylated flavone), demonstrating that COMT is a key enzyme involved in the synthesis of this compound.
28013276	3	73	theme	dimethylated	602:613	arg1	flavone					615:621	a dimethylated flavone	600:621	a dimethylated flavone	600:621	In this work, we determined that the comt (brown-midrib 3) mutant plants display a reduction of the flavonolignin unit derived from tricin (a dimethylated flavone), demonstrating that COMT is a key enzyme involved in the synthesis of this compound.
28013276	1	74	theme	coenzyme	147:154	arg1	A					156:156	Caffeoyl coenzyme A	138:156	Caffeoyl coenzyme A 3-O-methyltransferase (CCoAOMT)	138:188	Caffeoyl coenzyme A 3-O-methyltransferase (CCoAOMT) and caffeic acid-O-methyltransferase (COMT) are key enzymes in the biosynthesis of coniferyl and sinapyl alcohols, the precursors of guaiacyl (G) and syringyl (S) lignin subunits.
28013276	0	75	theme	Maize	51:55	arg1	Mutants					57:63	Maize Mutants	51:63	Maize Mutants Lacking 3'- and 5'-O-Methyltransferases	51:103	Changes in Cell Wall Polymers and Degradability in Maize Mutants Lacking 3'- and 5'-O-Methyltransferases Involved in Lignin Biosynthesis.
28013276	8	76	theme	ccoaomt1	1374:1381	arg1	degradable					1398:1407	degradable	1398:1407	degradable	1398:1407	Although stems from the three mutants display a similar increase of hemicelluloses, the effect on cell wall degradability varies, the cell walls of ccoaomt1 being the most degradable.
28013276	8	76	theme	ccoaomt1	1374:1381	arg1	walls					1365:1369	the cell walls	1356:1369	the cell walls of ccoaomt1	1356:1381	Although stems from the three mutants display a similar increase of hemicelluloses, the effect on cell wall degradability varies, the cell walls of ccoaomt1 being the most degradable.
28013276	9	77	theme	ccoaomt1	1508:1515	arg1	mutants					1522:1528	ccoaomt1 comt mutants	1508:1528	ccoaomt1 comt mutants	1508:1528	This suggests that the positive effect of lignin reduction on cell wall degradability of comt and ccoaomt1 comt mutants is counteracted by changes occurring in lignin composition, such as the decreased S/G ratio.
28013276	1	78	theme	A	156:156	arg1	enzymes					242:248	key enzymes	238:248	key enzymes	238:248	Caffeoyl coenzyme A 3-O-methyltransferase (CCoAOMT) and caffeic acid-O-methyltransferase (COMT) are key enzymes in the biosynthesis of coniferyl and sinapyl alcohols, the precursors of guaiacyl (G) and syringyl (S) lignin subunits.
28013276	1	78	theme	A	156:156	arg1	CCoAOMT					181:187	CCoAOMT	181:187	CCoAOMT	181:187	Caffeoyl coenzyme A 3-O-methyltransferase (CCoAOMT) and caffeic acid-O-methyltransferase (COMT) are key enzymes in the biosynthesis of coniferyl and sinapyl alcohols, the precursors of guaiacyl (G) and syringyl (S) lignin subunits.
28013276	1	78	theme	A	156:156	arg1	3-O-methyltransferase					158:178	Caffeoyl coenzyme A 3-O-methyltransferase	138:178	Caffeoyl coenzyme A 3-O-methyltransferase (CCoAOMT)	138:188	Caffeoyl coenzyme A 3-O-methyltransferase (CCoAOMT) and caffeic acid-O-methyltransferase (COMT) are key enzymes in the biosynthesis of coniferyl and sinapyl alcohols, the precursors of guaiacyl (G) and syringyl (S) lignin subunits.
28013276	1	78	theme	A	156:156	arg1	acid-O-methyltransferase					202:225	caffeic acid-O-methyltransferase	194:225	caffeic acid-O-methyltransferase (COMT)	194:232	Caffeoyl coenzyme A 3-O-methyltransferase (CCoAOMT) and caffeic acid-O-methyltransferase (COMT) are key enzymes in the biosynthesis of coniferyl and sinapyl alcohols, the precursors of guaiacyl (G) and syringyl (S) lignin subunits.
26993744	4	0	theme	galactosazide	606:618	arg1	blocks					629:634	galactosazide building blocks	606:634	galactosazide building blocks that were employed in highly stereoselective glycosylation reactions to furnish both the α- and β-configured linkages	606:752	The key GalNAc-GalNAc-GroP synthons, required for the synthesis, were generated from galactosazide building blocks that were employed in highly stereoselective glycosylation reactions to furnish both the α- and β-configured linkages.
26993744	1	1	theme	fragments	133:141	arg1	synthesis					64:72	The first synthesis	54:72	The first synthesis of different Enterococcus faecium wall teichoic acid (WTA) fragments	54:141	The first synthesis of different Enterococcus faecium wall teichoic acid (WTA) fragments is presented.
26993744	5	2	theme	isolated	822:829	arg1	material					831:838	the isolated material	818:838	the isolated material	818:838	By comparing the NMR spectra of the synthesized fragments with the isolated material it appears that the hereto undefined stereochemistry of the glycerol phosphate moiety is sn-glycerol-3-phosphate.
26993744	1	3	dep	different	77:85	arg1	Enterococcus					87:98	Enterococcus	87:98	Enterococcus	87:98	The first synthesis of different Enterococcus faecium wall teichoic acid (WTA) fragments is presented.
26993744	6	4	theme	molecular	1046:1054	arg1	level					1056:1060	the molecular level	1042:1060	the molecular level	1042:1060	The generated fragments will be valuable tools to study their immunological activity at the molecular level.
26993744	3	5	from	units	422:426	arg1	length					431:436	length	431:436	length	431:436	We assembled WTA fragments up to three repeating units in length, in two series that differ in the stereochemistry of the glycerolphosphate moiety.
26993744	4	6	theme	glycosylation	681:693	arg1	reactions					695:703	highly stereoselective glycosylation reactions	658:703	highly stereoselective glycosylation reactions	658:703	The key GalNAc-GalNAc-GroP synthons, required for the synthesis, were generated from galactosazide building blocks that were employed in highly stereoselective glycosylation reactions to furnish both the α- and β-configured linkages.
26993744	3	7	theme	repeating	412:420	arg1	units					422:426	three repeating units	406:426	three repeating units in length	406:436	We assembled WTA fragments up to three repeating units in length, in two series that differ in the stereochemistry of the glycerolphosphate moiety.
26993744	5	8	theme	undefined	867:875	arg1	stereochemistry					877:891	the hereto undefined stereochemistry	856:891	the hereto undefined stereochemistry of the glycerol phosphate moiety	856:924	By comparing the NMR spectra of the synthesized fragments with the isolated material it appears that the hereto undefined stereochemistry of the glycerol phosphate moiety is sn-glycerol-3-phosphate.
26993744	5	8	theme	undefined	867:875	arg1	sn-glycerol-3-phosphate					929:951	sn-glycerol-3-phosphate	929:951	sn-glycerol-3-phosphate	929:951	By comparing the NMR spectra of the synthesized fragments with the isolated material it appears that the hereto undefined stereochemistry of the glycerol phosphate moiety is sn-glycerol-3-phosphate.
26993744	4	9	theme	GalNAc-GalNAc-GroP	529:546	arg1	synthons					548:555	The key GalNAc-GalNAc-GroP synthons	521:555	The key GalNAc-GalNAc-GroP synthons	521:555	The key GalNAc-GalNAc-GroP synthons, required for the synthesis, were generated from galactosazide building blocks that were employed in highly stereoselective glycosylation reactions to furnish both the α- and β-configured linkages.
26993744	1	10	theme	first	58:62	arg1	synthesis					64:72	The first synthesis	54:72	The first synthesis of different Enterococcus faecium wall teichoic acid (WTA) fragments	54:141	The first synthesis of different Enterococcus faecium wall teichoic acid (WTA) fragments is presented.
26993744	6	11	theme	immunological	1016:1028	arg1	activity					1030:1037	their immunological activity	1010:1037	their immunological activity	1010:1037	The generated fragments will be valuable tools to study their immunological activity at the molecular level.
26993744	2	12	theme	-GalNAc-β	334:342	arg1	-6-					317:319	-6-	317:319	-6-(GalNAc-α(1-3)-GalNAc-β(1-2)-GroP)	317:353	The structure of these major cell wall components was elucidated recently and it was shown that these glycerolphosphate (GroP) based polymers are built up from -6-(GalNAc-α(1-3)-GalNAc-β(1-2)-GroP)- repeating units.
26993744	2	12	theme	-GalNAc-β	334:342	arg1	-GroP					348:352	GalNAc-α(1-3)-GalNAc-β(1-2)-GroP	321:352	GalNAc-α(1-3)-GalNAc-β(1-2)-GroP	321:352	The structure of these major cell wall components was elucidated recently and it was shown that these glycerolphosphate (GroP) based polymers are built up from -6-(GalNAc-α(1-3)-GalNAc-β(1-2)-GroP)- repeating units.
26993744	5	13	theme	moiety	919:924	arg1	stereochemistry					877:891	the hereto undefined stereochemistry	856:891	the hereto undefined stereochemistry of the glycerol phosphate moiety	856:924	By comparing the NMR spectra of the synthesized fragments with the isolated material it appears that the hereto undefined stereochemistry of the glycerol phosphate moiety is sn-glycerol-3-phosphate.
26993744	5	13	theme	moiety	919:924	arg1	sn-glycerol-3-phosphate					929:951	sn-glycerol-3-phosphate	929:951	sn-glycerol-3-phosphate	929:951	By comparing the NMR spectra of the synthesized fragments with the isolated material it appears that the hereto undefined stereochemistry of the glycerol phosphate moiety is sn-glycerol-3-phosphate.
26993744	2	14	theme	based	284:288	arg1	polymers					290:297	these glycerolphosphate (GroP) based polymers	253:297	these glycerolphosphate (GroP) based polymers	253:297	The structure of these major cell wall components was elucidated recently and it was shown that these glycerolphosphate (GroP) based polymers are built up from -6-(GalNAc-α(1-3)-GalNAc-β(1-2)-GroP)- repeating units.
26993744	5	15	theme	synthesized	791:801	arg1	fragments					803:811	the synthesized fragments	787:811	the synthesized fragments	787:811	By comparing the NMR spectra of the synthesized fragments with the isolated material it appears that the hereto undefined stereochemistry of the glycerol phosphate moiety is sn-glycerol-3-phosphate.
26993744	2	16	theme	repeating	356:364	arg1	units					366:370	repeating units	356:370	repeating units	356:370	The structure of these major cell wall components was elucidated recently and it was shown that these glycerolphosphate (GroP) based polymers are built up from -6-(GalNAc-α(1-3)-GalNAc-β(1-2)-GroP)- repeating units.
26993744	1	17	theme	different	77:85	arg1	fragments					133:141	different Enterococcus faecium wall teichoic acid (WTA) fragments	77:141	different Enterococcus faecium wall teichoic acid (WTA) fragments	77:141	The first synthesis of different Enterococcus faecium wall teichoic acid (WTA) fragments is presented.
26993744	3	18	dep	units	422:426	arg1	up					400:401	up	400:401	up	400:401	We assembled WTA fragments up to three repeating units in length, in two series that differ in the stereochemistry of the glycerolphosphate moiety.
26993744	4	19	theme	stereoselective	665:679	arg1	reactions					695:703	highly stereoselective glycosylation reactions	658:703	highly stereoselective glycosylation reactions	658:703	The key GalNAc-GalNAc-GroP synthons, required for the synthesis, were generated from galactosazide building blocks that were employed in highly stereoselective glycosylation reactions to furnish both the α- and β-configured linkages.
26993744	0	20	theme	E.	13:14	arg1	fragments					43:51	E. faecium wall teichoic acid fragments	13:51	E. faecium wall teichoic acid fragments	13:51	Synthesis of E. faecium wall teichoic acid fragments.
26993744	5	21	theme	phosphate	909:917	arg1	moiety					919:924	the glycerol phosphate moiety	896:924	the glycerol phosphate moiety	896:924	By comparing the NMR spectra of the synthesized fragments with the isolated material it appears that the hereto undefined stereochemistry of the glycerol phosphate moiety is sn-glycerol-3-phosphate.
26993744	3	22	theme	WTA	386:388	arg1	fragments					390:398	WTA fragments	386:398	WTA fragments up to three repeating units in length	386:436	We assembled WTA fragments up to three repeating units in length, in two series that differ in the stereochemistry of the glycerolphosphate moiety.
26993744	0	23	theme	teichoic	29:36	arg1	fragments					43:51	E. faecium wall teichoic acid fragments	13:51	E. faecium wall teichoic acid fragments	13:51	Synthesis of E. faecium wall teichoic acid fragments.
26993744	4	24	theme	building	620:627	arg1	blocks					629:634	galactosazide building blocks	606:634	galactosazide building blocks that were employed in highly stereoselective glycosylation reactions to furnish both the α- and β-configured linkages	606:752	The key GalNAc-GalNAc-GroP synthons, required for the synthesis, were generated from galactosazide building blocks that were employed in highly stereoselective glycosylation reactions to furnish both the α- and β-configured linkages.
26993744	5	25	theme	NMR	772:774	arg1	spectra					776:782	the NMR spectra	768:782	the NMR spectra of the synthesized fragments	768:811	By comparing the NMR spectra of the synthesized fragments with the isolated material it appears that the hereto undefined stereochemistry of the glycerol phosphate moiety is sn-glycerol-3-phosphate.
26993744	5	26	theme	fragments	803:811	arg1	spectra					776:782	the NMR spectra	768:782	the NMR spectra of the synthesized fragments	768:811	By comparing the NMR spectra of the synthesized fragments with the isolated material it appears that the hereto undefined stereochemistry of the glycerol phosphate moiety is sn-glycerol-3-phosphate.
26993744	4	27	dep	linkages	745:752	arg1	both					716:719	both	716:719	both	716:719	The key GalNAc-GalNAc-GroP synthons, required for the synthesis, were generated from galactosazide building blocks that were employed in highly stereoselective glycosylation reactions to furnish both the α- and β-configured linkages.
26993744	0	28	theme	wall	24:27	arg1	fragments					43:51	E. faecium wall teichoic acid fragments	13:51	E. faecium wall teichoic acid fragments	13:51	Synthesis of E. faecium wall teichoic acid fragments.
26993744	3	29	theme	glycerolphosphate	495:511	arg1	moiety					513:518	the glycerolphosphate moiety	491:518	the glycerolphosphate moiety	491:518	We assembled WTA fragments up to three repeating units in length, in two series that differ in the stereochemistry of the glycerolphosphate moiety.
26993744	6	30	theme	valuable	986:993	arg1	fragments					968:976	The generated fragments	954:976	The generated fragments	954:976	The generated fragments will be valuable tools to study their immunological activity at the molecular level.
26993744	6	30	theme	valuable	986:993	arg1	tools					995:999	valuable tools	986:999	valuable tools	986:999	The generated fragments will be valuable tools to study their immunological activity at the molecular level.
26993744	0	31	theme	fragments	43:51	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of E. faecium wall teichoic acid fragments	0:51	Synthesis of E. faecium wall teichoic acid fragments.
26993744	2	32	theme	components	196:205	arg1	structure					161:169	The structure	157:169	The structure of these major cell wall components	157:205	The structure of these major cell wall components was elucidated recently and it was shown that these glycerolphosphate (GroP) based polymers are built up from -6-(GalNAc-α(1-3)-GalNAc-β(1-2)-GroP)- repeating units.
26993744	1	33	theme	wall	108:111	arg1	acid					122:125	wall teichoic acid	108:125	different Enterococcus faecium wall teichoic acid (WTA) fragments	77:141	The first synthesis of different Enterococcus faecium wall teichoic acid (WTA) fragments is presented.
26993744	1	33	theme	wall	108:111	arg1	WTA					128:130	WTA	128:130	WTA	128:130	The first synthesis of different Enterococcus faecium wall teichoic acid (WTA) fragments is presented.
26993744	0	34	theme	acid	38:41	arg1	fragments					43:51	E. faecium wall teichoic acid fragments	13:51	E. faecium wall teichoic acid fragments	13:51	Synthesis of E. faecium wall teichoic acid fragments.
26993744	2	35	theme	cell	186:189	arg1	components					196:205	these major cell wall components	174:205	these major cell wall components	174:205	The structure of these major cell wall components was elucidated recently and it was shown that these glycerolphosphate (GroP) based polymers are built up from -6-(GalNAc-α(1-3)-GalNAc-β(1-2)-GroP)- repeating units.
26993744	2	36	theme	wall	191:194	arg1	components					196:205	these major cell wall components	174:205	these major cell wall components	174:205	The structure of these major cell wall components was elucidated recently and it was shown that these glycerolphosphate (GroP) based polymers are built up from -6-(GalNAc-α(1-3)-GalNAc-β(1-2)-GroP)- repeating units.
26993744	1	37	theme	teichoic	113:120	arg1	acid					122:125	wall teichoic acid	108:125	different Enterococcus faecium wall teichoic acid (WTA) fragments	77:141	The first synthesis of different Enterococcus faecium wall teichoic acid (WTA) fragments is presented.
26993744	1	37	theme	teichoic	113:120	arg1	WTA					128:130	WTA	128:130	WTA	128:130	The first synthesis of different Enterococcus faecium wall teichoic acid (WTA) fragments is presented.
26993744	2	38	theme	major	180:184	arg1	components					196:205	these major cell wall components	174:205	these major cell wall components	174:205	The structure of these major cell wall components was elucidated recently and it was shown that these glycerolphosphate (GroP) based polymers are built up from -6-(GalNAc-α(1-3)-GalNAc-β(1-2)-GroP)- repeating units.
26993744	4	39	theme	β-configured	732:743	arg1	linkages					745:752	both the α- and β-configured linkages	716:752	linkages	745:752	The key GalNAc-GalNAc-GroP synthons, required for the synthesis, were generated from galactosazide building blocks that were employed in highly stereoselective glycosylation reactions to furnish both the α- and β-configured linkages.
26993744	1	40	theme	acid	122:125	arg1	fragments					133:141	different Enterococcus faecium wall teichoic acid (WTA) fragments	77:141	different Enterococcus faecium wall teichoic acid (WTA) fragments	77:141	The first synthesis of different Enterococcus faecium wall teichoic acid (WTA) fragments is presented.
26993744	0	41	dep	E.	13:14	arg1	faecium					16:22	faecium	16:22	faecium	16:22	Synthesis of E. faecium wall teichoic acid fragments.
26993744	2	42	dep	based	284:288	arg1	GroP					278:281	GroP	278:281	GroP	278:281	The structure of these major cell wall components was elucidated recently and it was shown that these glycerolphosphate (GroP) based polymers are built up from -6-(GalNAc-α(1-3)-GalNAc-β(1-2)-GroP)- repeating units.
26993744	2	42	dep	based	284:288	arg1	glycerolphosphate					259:275	glycerolphosphate	259:275	glycerolphosphate (GroP)	259:282	The structure of these major cell wall components was elucidated recently and it was shown that these glycerolphosphate (GroP) based polymers are built up from -6-(GalNAc-α(1-3)-GalNAc-β(1-2)-GroP)- repeating units.
26993744	3	43	theme	moiety	513:518	arg1	stereochemistry					472:486	the stereochemistry	468:486	the stereochemistry of the glycerolphosphate moiety	468:518	We assembled WTA fragments up to three repeating units in length, in two series that differ in the stereochemistry of the glycerolphosphate moiety.
26993744	1	44	dep	Enterococcus	87:98	arg1	faecium					100:106	faecium	100:106	faecium	100:106	The first synthesis of different Enterococcus faecium wall teichoic acid (WTA) fragments is presented.
26993744	4	45	theme	key	525:527	arg1	synthons					548:555	The key GalNAc-GalNAc-GroP synthons	521:555	The key GalNAc-GalNAc-GroP synthons	521:555	The key GalNAc-GalNAc-GroP synthons, required for the synthesis, were generated from galactosazide building blocks that were employed in highly stereoselective glycosylation reactions to furnish both the α- and β-configured linkages.
26993744	5	46	theme	glycerol	900:907	arg1	moiety					919:924	the glycerol phosphate moiety	896:924	the glycerol phosphate moiety	896:924	By comparing the NMR spectra of the synthesized fragments with the isolated material it appears that the hereto undefined stereochemistry of the glycerol phosphate moiety is sn-glycerol-3-phosphate.
26993744	4	47	theme	α-	725:726	arg1	linkages					745:752	both the α- and β-configured linkages	716:752	linkages	745:752	The key GalNAc-GalNAc-GroP synthons, required for the synthesis, were generated from galactosazide building blocks that were employed in highly stereoselective glycosylation reactions to furnish both the α- and β-configured linkages.
26993744	6	48	theme	generated	958:966	arg1	fragments					968:976	The generated fragments	954:976	The generated fragments	954:976	The generated fragments will be valuable tools to study their immunological activity at the molecular level.
26993744	6	48	theme	generated	958:966	arg1	tools					995:999	valuable tools	986:999	valuable tools	986:999	The generated fragments will be valuable tools to study their immunological activity at the molecular level.
27083800	0	0	from	actuation	20:28	arg1	oxide					59:63	chitosan/reduced graphene oxide	33:63	chitosan/reduced graphene oxide	33:63	Optically triggered actuation in chitosan/reduced graphene oxide nanocomposites.
27083800	9	1	theme	applied	847:853	arg1	pre-strain					855:864	applied pre-strain	847:864	applied pre-strain	847:864	The actuation behaviour can be tuned either by altering the RGO content or applied pre-strain.
27083800	6	2	theme	Photomechanical	587:601	arg1	stress					603:608	Photomechanical stress	587:608	Photomechanical stress as high as 695kPa	587:626	Photomechanical stress as high as 695kPa was achieved with 4wt.
27083800	1	3	contain	have	144:147	arg1	actuators					96:104	Bio-compatible actuators	81:104	Bio-compatible actuators which can work under optical stimulus	81:142	Bio-compatible actuators which can work under optical stimulus have great future in bio-medical applications.
27083800	1	3	contain	have	144:147	arg2	future					155:160	future	155:160	future	155:160	Bio-compatible actuators which can work under optical stimulus have great future in bio-medical applications.
27083800	2	4	theme	graphene	222:229	arg1	RGO					238:240	RGO	238:240	RGO	238:240	In this work, chitosan/reduced graphene oxide (RGO) nanocomposite optical actuators were developed through a simple solvent casting technique.
27083800	2	4	theme	graphene	222:229	arg1	oxide					231:235	chitosan/reduced graphene oxide	205:235	chitosan/reduced graphene oxide (RGO) nanocomposite optical actuators	205:273	In this work, chitosan/reduced graphene oxide (RGO) nanocomposite optical actuators were developed through a simple solvent casting technique.
27083800	9	5	theme	RGO	832:834	arg1	content					836:842	the RGO content	828:842	the RGO content	828:842	The actuation behaviour can be tuned either by altering the RGO content or applied pre-strain.
27083800	2	6	theme	casting	315:321	arg1	technique					323:331	a simple solvent casting technique	298:331	a simple solvent casting technique	298:331	In this work, chitosan/reduced graphene oxide (RGO) nanocomposite optical actuators were developed through a simple solvent casting technique.
27083800	3	7	theme	IR	404:405	arg1	illumination					407:418	IR illumination	404:418	IR illumination	404:418	The photomechanical actuation of the composites is demonstrated under IR illumination.
27083800	1	8	theme	Bio-compatible	81:94	arg1	actuators					96:104	Bio-compatible actuators	81:104	Bio-compatible actuators which can work under optical stimulus	81:142	Bio-compatible actuators which can work under optical stimulus have great future in bio-medical applications.
27083800	2	9	theme	solvent	307:313	arg1	technique					323:331	a simple solvent casting technique	298:331	a simple solvent casting technique	298:331	In this work, chitosan/reduced graphene oxide (RGO) nanocomposite optical actuators were developed through a simple solvent casting technique.
27083800	2	10	theme	simple	300:305	arg1	technique					323:331	a simple solvent casting technique	298:331	a simple solvent casting technique	298:331	In this work, chitosan/reduced graphene oxide (RGO) nanocomposite optical actuators were developed through a simple solvent casting technique.
27083800	9	11	dep	altering	819:826	arg1	either					809:814	either	809:814	either	809:814	The actuation behaviour can be tuned either by altering the RGO content or applied pre-strain.
27083800	0	12	theme	triggered	10:18	arg1	actuation					20:28	Optically triggered actuation	0:28	Optically triggered actuation in chitosan/reduced graphene oxide	0:63	Optically triggered actuation in chitosan/reduced graphene oxide nanocomposites.
27083800	9	13	theme	actuation	776:784	arg1	behaviour					786:794	The actuation behaviour	772:794	The actuation behaviour	772:794	The actuation behaviour can be tuned either by altering the RGO content or applied pre-strain.
27083800	9	14	dep	tuned	803:807	arg1	altering					819:826	altering	819:826	altering the RGO content or applied pre-strain	819:864	The actuation behaviour can be tuned either by altering the RGO content or applied pre-strain.
27083800	8	15	theme	photomechanical	710:724	arg1	stress					726:731	the photomechanical stress	706:731	the photomechanical stress	706:731	Contrary to some other reported systems, the photomechanical stress decreased with the applied pre-strain.
27083800	8	16	theme	other	682:686	arg1	systems					697:703	some other reported systems	677:703	some other reported systems	677:703	Contrary to some other reported systems, the photomechanical stress decreased with the applied pre-strain.
27083800	8	17	theme	applied	752:758	arg1	pre-strain					760:769	the applied pre-strain	748:769	the applied pre-strain	748:769	Contrary to some other reported systems, the photomechanical stress decreased with the applied pre-strain.
27083800	1	18	theme	optical	127:133	arg1	stimulus					135:142	optical stimulus	127:142	optical stimulus	127:142	Bio-compatible actuators which can work under optical stimulus have great future in bio-medical applications.
27083800	0	19	theme	graphene	50:57	arg1	oxide					59:63	chitosan/reduced graphene oxide	33:63	chitosan/reduced graphene oxide	33:63	Optically triggered actuation in chitosan/reduced graphene oxide nanocomposites.
27083800	2	20	theme	optical	257:263	arg1	actuators					265:273	chitosan/reduced graphene oxide (RGO) nanocomposite optical actuators	205:273	chitosan/reduced graphene oxide (RGO) nanocomposite optical actuators	205:273	In this work, chitosan/reduced graphene oxide (RGO) nanocomposite optical actuators were developed through a simple solvent casting technique.
27083800	0	21	theme	chitosan/reduced	33:48	arg1	oxide					59:63	chitosan/reduced graphene oxide	33:63	chitosan/reduced graphene oxide	33:63	Optically triggered actuation in chitosan/reduced graphene oxide nanocomposites.
27083800	2	22	theme	nanocomposite	243:255	arg1	actuators					265:273	chitosan/reduced graphene oxide (RGO) nanocomposite optical actuators	205:273	chitosan/reduced graphene oxide (RGO) nanocomposite optical actuators	205:273	In this work, chitosan/reduced graphene oxide (RGO) nanocomposite optical actuators were developed through a simple solvent casting technique.
27083800	3	23	theme	photomechanical	338:352	arg1	actuation					354:362	The photomechanical actuation	334:362	The photomechanical actuation of the composites	334:380	The photomechanical actuation of the composites is demonstrated under IR illumination.
27083800	2	24	theme	oxide	231:235	arg1	actuators					265:273	chitosan/reduced graphene oxide (RGO) nanocomposite optical actuators	205:273	chitosan/reduced graphene oxide (RGO) nanocomposite optical actuators	205:273	In this work, chitosan/reduced graphene oxide (RGO) nanocomposite optical actuators were developed through a simple solvent casting technique.
27083800	4	25	theme	IR	481:482	arg1	light					484:488	IR light	481:488	IR light	481:488	All samples exhibited contraction in length when exposed to IR light.
27083800	8	26	theme	reported	688:695	arg1	systems					697:703	some other reported systems	677:703	some other reported systems	677:703	Contrary to some other reported systems, the photomechanical stress decreased with the applied pre-strain.
27083800	6	27	theme	high	613:616	arg1	stress					603:608	Photomechanical stress	587:608	Photomechanical stress as high as 695kPa	587:626	Photomechanical stress as high as 695kPa was achieved with 4wt.
27083800	2	28	theme	chitosan/reduced	205:220	arg1	RGO					238:240	RGO	238:240	RGO	238:240	In this work, chitosan/reduced graphene oxide (RGO) nanocomposite optical actuators were developed through a simple solvent casting technique.
27083800	2	28	theme	chitosan/reduced	205:220	arg1	oxide					231:235	chitosan/reduced graphene oxide	205:235	chitosan/reduced graphene oxide (RGO) nanocomposite optical actuators	205:273	In this work, chitosan/reduced graphene oxide (RGO) nanocomposite optical actuators were developed through a simple solvent casting technique.
27083800	5	29	theme	RGO	568:570	arg1	concentration					572:584	RGO concentration	568:584	RGO concentration	568:584	The photomechanical stress and strain were found to increase with increasing RGO concentration.
27083800	5	30	theme	photomechanical	495:509	arg1	stress					511:516	The photomechanical stress	491:516	The photomechanical stress	491:516	The photomechanical stress and strain were found to increase with increasing RGO concentration.
27083800	3	31	theme	composites	371:380	arg1	actuation					354:362	The photomechanical actuation	334:362	The photomechanical actuation of the composites	334:380	The photomechanical actuation of the composites is demonstrated under IR illumination.
27083800	1	32	theme	bio-medical	165:175	arg1	applications					177:188	bio-medical applications	165:188	bio-medical applications	165:188	Bio-compatible actuators which can work under optical stimulus have great future in bio-medical applications.
24792554	2	0	theme	host	487:490	arg1	cells					492:496	eukaryotic host cells	476:496	eukaryotic host cells	476:496	Here, we describe detailed experimental methods for the extraction, purification, and analysis of wall teichoic acids (WTA), which assume important roles as major constituents of Gram-positive cell walls, such as mediating interaction with cell wall-associated proteins, eukaryotic host cells, and bacteriophages.
24792554	1	1	theme	cell	183:186	arg1	components					193:202	cell wall components	183:202	cell wall components	183:202	Understanding molecular interactions of bacteria with their environment requires the purification and characterization of cell wall components.
24792554	2	2	theme	important	343:351	arg1	roles					353:357	important roles	343:357	important roles	343:357	Here, we describe detailed experimental methods for the extraction, purification, and analysis of wall teichoic acids (WTA), which assume important roles as major constituents of Gram-positive cell walls, such as mediating interaction with cell wall-associated proteins, eukaryotic host cells, and bacteriophages.
24792554	2	2	theme	important	343:351	arg1	constituents					368:379	major constituents	362:379	major constituents of Gram-positive cell walls	362:407	Here, we describe detailed experimental methods for the extraction, purification, and analysis of wall teichoic acids (WTA), which assume important roles as major constituents of Gram-positive cell walls, such as mediating interaction with cell wall-associated proteins, eukaryotic host cells, and bacteriophages.
24792554	2	3	theme	wall	303:306	arg1	acids					317:321	wall teichoic acids	303:321	wall teichoic acids (WTA)	303:327	Here, we describe detailed experimental methods for the extraction, purification, and analysis of wall teichoic acids (WTA), which assume important roles as major constituents of Gram-positive cell walls, such as mediating interaction with cell wall-associated proteins, eukaryotic host cells, and bacteriophages.
24792554	2	3	theme	wall	303:306	arg1	WTA					324:326	WTA	324:326	WTA	324:326	Here, we describe detailed experimental methods for the extraction, purification, and analysis of wall teichoic acids (WTA), which assume important roles as major constituents of Gram-positive cell walls, such as mediating interaction with cell wall-associated proteins, eukaryotic host cells, and bacteriophages.
24792554	3	4	theme	compositional	560:572	arg1	characterization					578:593	compositional WTA characterization	560:593	compositional WTA characterization	560:593	Specifically, we present a procedure for compositional WTA characterization to study large diversity of carbohydrate substitution on Listeria monocytogenes WTA.
24792554	1	5	dep	purification	146:157	arg1	the					142:144	the	142:144	the	142:144	Understanding molecular interactions of bacteria with their environment requires the purification and characterization of cell wall components.
24792554	1	6	theme	molecular	75:83	arg1	interactions					85:96	molecular interactions	75:96	molecular interactions of bacteria with their environment	75:131	Understanding molecular interactions of bacteria with their environment requires the purification and characterization of cell wall components.
24792554	1	7	theme	wall	188:191	arg1	components					193:202	cell wall components	183:202	cell wall components	183:202	Understanding molecular interactions of bacteria with their environment requires the purification and characterization of cell wall components.
24792554	2	8	theme	wall-associated	450:464	arg1	proteins					466:473	cell wall-associated proteins	445:473	cell wall-associated proteins	445:473	Here, we describe detailed experimental methods for the extraction, purification, and analysis of wall teichoic acids (WTA), which assume important roles as major constituents of Gram-positive cell walls, such as mediating interaction with cell wall-associated proteins, eukaryotic host cells, and bacteriophages.
24792554	1	9	theme	components	193:202	arg1	characterization					163:178	characterization	163:178	characterization	163:178	Understanding molecular interactions of bacteria with their environment requires the purification and characterization of cell wall components.
24792554	1	9	theme	components	193:202	arg1	purification					146:157	purification	146:157	purification	146:157	Understanding molecular interactions of bacteria with their environment requires the purification and characterization of cell wall components.
24792554	0	10	theme	Listeria	4:11	arg1	wall					18:21	The Listeria cell wall	0:21	The Listeria cell wall	0:21	The Listeria cell wall and associated carbohydrate polymers.
24792554	3	11	theme	WTA	574:576	arg1	characterization					578:593	compositional WTA characterization	560:593	compositional WTA characterization	560:593	Specifically, we present a procedure for compositional WTA characterization to study large diversity of carbohydrate substitution on Listeria monocytogenes WTA.
24792554	4	12	from	bacteria	749:756	arg1	WTA					734:736	WTA	734:736	WTA from other bacteria	734:756	This protocol may also be used and adapted to analyze WTA from other bacteria.
24792554	1	13	theme	bacteria	101:108	arg1	interactions					85:96	molecular interactions	75:96	molecular interactions of bacteria with their environment	75:131	Understanding molecular interactions of bacteria with their environment requires the purification and characterization of cell wall components.
24792554	4	14	theme	other	743:747	arg1	bacteria					749:756	other bacteria	743:756	other bacteria	743:756	This protocol may also be used and adapted to analyze WTA from other bacteria.
24792554	4	15	used	used	706:709	arg2	protocol					685:692	This protocol	680:692	This protocol	680:692	This protocol may also be used and adapted to analyze WTA from other bacteria.
24792554	2	16	theme	acids	317:321	arg1	purification					273:284	purification	273:284	purification	273:284	Here, we describe detailed experimental methods for the extraction, purification, and analysis of wall teichoic acids (WTA), which assume important roles as major constituents of Gram-positive cell walls, such as mediating interaction with cell wall-associated proteins, eukaryotic host cells, and bacteriophages.
24792554	2	16	theme	acids	317:321	arg1	analysis					291:298	analysis	291:298	analysis	291:298	Here, we describe detailed experimental methods for the extraction, purification, and analysis of wall teichoic acids (WTA), which assume important roles as major constituents of Gram-positive cell walls, such as mediating interaction with cell wall-associated proteins, eukaryotic host cells, and bacteriophages.
24792554	2	16	theme	acids	317:321	arg1	extraction					261:270	the extraction	257:270	the extraction	257:270	Here, we describe detailed experimental methods for the extraction, purification, and analysis of wall teichoic acids (WTA), which assume important roles as major constituents of Gram-positive cell walls, such as mediating interaction with cell wall-associated proteins, eukaryotic host cells, and bacteriophages.
24792554	0	17	theme	cell	13:16	arg1	wall					18:21	The Listeria cell wall	0:21	The Listeria cell wall	0:21	The Listeria cell wall and associated carbohydrate polymers.
24792554	2	18	theme	teichoic	308:315	arg1	acids					317:321	wall teichoic acids	303:321	wall teichoic acids (WTA)	303:327	Here, we describe detailed experimental methods for the extraction, purification, and analysis of wall teichoic acids (WTA), which assume important roles as major constituents of Gram-positive cell walls, such as mediating interaction with cell wall-associated proteins, eukaryotic host cells, and bacteriophages.
24792554	2	18	theme	teichoic	308:315	arg1	WTA					324:326	WTA	324:326	WTA	324:326	Here, we describe detailed experimental methods for the extraction, purification, and analysis of wall teichoic acids (WTA), which assume important roles as major constituents of Gram-positive cell walls, such as mediating interaction with cell wall-associated proteins, eukaryotic host cells, and bacteriophages.
24792554	2	19	theme	eukaryotic	476:485	arg1	cells					492:496	eukaryotic host cells	476:496	eukaryotic host cells	476:496	Here, we describe detailed experimental methods for the extraction, purification, and analysis of wall teichoic acids (WTA), which assume important roles as major constituents of Gram-positive cell walls, such as mediating interaction with cell wall-associated proteins, eukaryotic host cells, and bacteriophages.
24792554	3	20	theme	large	604:608	arg1	diversity					610:618	large diversity	604:618	large diversity of carbohydrate substitution on Listeria monocytogenes WTA	604:677	Specifically, we present a procedure for compositional WTA characterization to study large diversity of carbohydrate substitution on Listeria monocytogenes WTA.
24792554	3	21	from	diversity	610:618	arg1	monocytogenes					661:673	Listeria monocytogenes	652:673	Listeria monocytogenes WTA	652:677	Specifically, we present a procedure for compositional WTA characterization to study large diversity of carbohydrate substitution on Listeria monocytogenes WTA.
24792554	2	22	theme	cell	398:401	arg1	walls					403:407	Gram-positive cell walls	384:407	Gram-positive cell walls	384:407	Here, we describe detailed experimental methods for the extraction, purification, and analysis of wall teichoic acids (WTA), which assume important roles as major constituents of Gram-positive cell walls, such as mediating interaction with cell wall-associated proteins, eukaryotic host cells, and bacteriophages.
24792554	1	23	with	interactions	85:96	arg1	environment					121:131	their environment	115:131	their environment	115:131	Understanding molecular interactions of bacteria with their environment requires the purification and characterization of cell wall components.
24792554	3	24	theme	Listeria	652:659	arg1	monocytogenes					661:673	Listeria monocytogenes	652:673	Listeria monocytogenes WTA	652:677	Specifically, we present a procedure for compositional WTA characterization to study large diversity of carbohydrate substitution on Listeria monocytogenes WTA.
24792554	2	25	with	interaction	428:438	arg1	proteins					466:473	cell wall-associated proteins	445:473	cell wall-associated proteins	445:473	Here, we describe detailed experimental methods for the extraction, purification, and analysis of wall teichoic acids (WTA), which assume important roles as major constituents of Gram-positive cell walls, such as mediating interaction with cell wall-associated proteins, eukaryotic host cells, and bacteriophages.
24792554	2	25	with	interaction	428:438	arg1	cells					492:496	eukaryotic host cells	476:496	eukaryotic host cells	476:496	Here, we describe detailed experimental methods for the extraction, purification, and analysis of wall teichoic acids (WTA), which assume important roles as major constituents of Gram-positive cell walls, such as mediating interaction with cell wall-associated proteins, eukaryotic host cells, and bacteriophages.
24792554	2	25	with	interaction	428:438	arg1	bacteriophages					503:516	bacteriophages	503:516	bacteriophages	503:516	Here, we describe detailed experimental methods for the extraction, purification, and analysis of wall teichoic acids (WTA), which assume important roles as major constituents of Gram-positive cell walls, such as mediating interaction with cell wall-associated proteins, eukaryotic host cells, and bacteriophages.
24792554	2	26	theme	walls	403:407	arg1	roles					353:357	important roles	343:357	important roles	343:357	Here, we describe detailed experimental methods for the extraction, purification, and analysis of wall teichoic acids (WTA), which assume important roles as major constituents of Gram-positive cell walls, such as mediating interaction with cell wall-associated proteins, eukaryotic host cells, and bacteriophages.
24792554	2	26	theme	walls	403:407	arg1	constituents					368:379	major constituents	362:379	major constituents of Gram-positive cell walls	362:407	Here, we describe detailed experimental methods for the extraction, purification, and analysis of wall teichoic acids (WTA), which assume important roles as major constituents of Gram-positive cell walls, such as mediating interaction with cell wall-associated proteins, eukaryotic host cells, and bacteriophages.
24792554	0	27	theme	carbohydrate	38:49	arg1	polymers					51:58	carbohydrate polymers	38:58	carbohydrate polymers	38:58	The Listeria cell wall and associated carbohydrate polymers.
24792554	2	28	theme	detailed	223:230	arg1	methods					245:251	detailed experimental methods	223:251	detailed experimental methods for the extraction, purification, and analysis of wall teichoic acids (WTA), which assume important roles as major constituents of Gram-positive cell walls, such as mediating interaction with cell wall-associated proteins, eukaryotic host cells, and bacteriophages	223:516	Here, we describe detailed experimental methods for the extraction, purification, and analysis of wall teichoic acids (WTA), which assume important roles as major constituents of Gram-positive cell walls, such as mediating interaction with cell wall-associated proteins, eukaryotic host cells, and bacteriophages.
24792554	2	29	theme	experimental	232:243	arg1	methods					245:251	detailed experimental methods	223:251	detailed experimental methods for the extraction, purification, and analysis of wall teichoic acids (WTA), which assume important roles as major constituents of Gram-positive cell walls, such as mediating interaction with cell wall-associated proteins, eukaryotic host cells, and bacteriophages	223:516	Here, we describe detailed experimental methods for the extraction, purification, and analysis of wall teichoic acids (WTA), which assume important roles as major constituents of Gram-positive cell walls, such as mediating interaction with cell wall-associated proteins, eukaryotic host cells, and bacteriophages.
24792554	3	30	theme	substitution	636:647	arg1	diversity					610:618	large diversity	604:618	large diversity of carbohydrate substitution on Listeria monocytogenes WTA	604:677	Specifically, we present a procedure for compositional WTA characterization to study large diversity of carbohydrate substitution on Listeria monocytogenes WTA.
24792554	3	31	dep	monocytogenes	661:673	arg1	WTA					675:677	WTA	675:677	Listeria monocytogenes WTA	652:677	Specifically, we present a procedure for compositional WTA characterization to study large diversity of carbohydrate substitution on Listeria monocytogenes WTA.
24792554	2	32	theme	major	362:366	arg1	roles					353:357	important roles	343:357	important roles	343:357	Here, we describe detailed experimental methods for the extraction, purification, and analysis of wall teichoic acids (WTA), which assume important roles as major constituents of Gram-positive cell walls, such as mediating interaction with cell wall-associated proteins, eukaryotic host cells, and bacteriophages.
24792554	2	32	theme	major	362:366	arg1	constituents					368:379	major constituents	362:379	major constituents of Gram-positive cell walls	362:407	Here, we describe detailed experimental methods for the extraction, purification, and analysis of wall teichoic acids (WTA), which assume important roles as major constituents of Gram-positive cell walls, such as mediating interaction with cell wall-associated proteins, eukaryotic host cells, and bacteriophages.
24792554	2	33	theme	Gram-positive	384:396	arg1	walls					403:407	Gram-positive cell walls	384:407	Gram-positive cell walls	384:407	Here, we describe detailed experimental methods for the extraction, purification, and analysis of wall teichoic acids (WTA), which assume important roles as major constituents of Gram-positive cell walls, such as mediating interaction with cell wall-associated proteins, eukaryotic host cells, and bacteriophages.
24792554	3	34	theme	carbohydrate	623:634	arg1	substitution					636:647	carbohydrate substitution	623:647	carbohydrate substitution	623:647	Specifically, we present a procedure for compositional WTA characterization to study large diversity of carbohydrate substitution on Listeria monocytogenes WTA.
24792554	2	35	theme	cell	445:448	arg1	proteins					466:473	cell wall-associated proteins	445:473	cell wall-associated proteins	445:473	Here, we describe detailed experimental methods for the extraction, purification, and analysis of wall teichoic acids (WTA), which assume important roles as major constituents of Gram-positive cell walls, such as mediating interaction with cell wall-associated proteins, eukaryotic host cells, and bacteriophages.
24677767	10	0	theme	CSCRG1	1402:1407	arg1	scaffolds					1409:1417	the pH. CSCRG1 scaffolds	1394:1417	the pH. CSCRG1 scaffolds appeared to have higher hydrophilicity and therefore higher water absorption capacity	1394:1503	The scaffolds obtained presented large and interconnected pores, being equally sensitive to changes in the pH. CSCRG1 scaffolds appeared to have higher hydrophilicity and therefore higher water absorption capacity.
24677767	6	1	theme	influence	750:758	arg1	compositions					707:718	The chemical compositions	694:718	The chemical compositions of the PECs, as well as their influence in the final structure of the scaffolds	694:798	The chemical compositions of the PECs, as well as their influence in the final structure of the scaffolds were meticulously studied.
24677767	8	2	from	values	1088:1093	arg1	stable					1075:1080	stable	1075:1080	stable	1075:1080	Results showed that the PECs produced were stable at pH values of 7.4 and under but dissolved as the pH increased to nonphysiological values of 9 and 11.
24677767	5	3	theme	2:1	652:654	arg1	ratios					628:633	CRG molar ratios	618:633	CRG molar ratios of 1:1 (CSCRG1), 2:1 (CSCRG2), and 3:1 (CSCRG3)	618:681	CS:CRG molar ratios of 1:1 (CSCRG1), 2:1 (CSCRG2), and 3:1 (CSCRG3) were used.
24677767	8	4	theme	pH	1085:1086	arg1	values					1088:1093	pH values	1085:1093	pH values of 7.4	1085:1100	Results showed that the PECs produced were stable at pH values of 7.4 and under but dissolved as the pH increased to nonphysiological values of 9 and 11.
24677767	7	5	from	responsiveness	847:860	arg1	values					916:921	the physiological pH values	895:921	the physiological pH values of 7.4 (simulating normal physiological conditions) and 4.5 (simulating inflammatory response)	895:1016	In addition, the pH responsiveness of the PECs in a range including the physiological pH values of 7.4 (simulating normal physiological conditions) and 4.5 (simulating inflammatory response) was assessed.
24677767	7	5	from	responsiveness	847:860	arg1	range					879:883	a range	877:883	a range including the physiological pH values of 7.4 (simulating normal physiological conditions) and 4.5 (simulating inflammatory response)	877:1016	In addition, the pH responsiveness of the PECs in a range including the physiological pH values of 7.4 (simulating normal physiological conditions) and 4.5 (simulating inflammatory response) was assessed.
24677767	2	6	theme	crosslinking	304:315	arg1	agents					317:322	crosslinking agents	304:322	crosslinking agents	304:322	These substances are obtained in aqueous medium without the need for crosslinking agents.
24677767	1	7	theme	tissue	202:207	arg1	engineering					209:219	tissue engineering	202:219	tissue engineering	202:219	Polyelectrolyte complexes (PECs) represent promising materials for drug delivery and tissue engineering applications.
24677767	8	8	from	under	1106:1110	arg1	stable					1075:1080	stable	1075:1080	stable	1075:1080	Results showed that the PECs produced were stable at pH values of 7.4 and under but dissolved as the pH increased to nonphysiological values of 9 and 11.
24677767	10	9	theme	water	1479:1483	arg1	absorption					1485:1494	higher water absorption	1472:1494	higher water absorption capacity	1472:1503	The scaffolds obtained presented large and interconnected pores, being equally sensitive to changes in the pH. CSCRG1 scaffolds appeared to have higher hydrophilicity and therefore higher water absorption capacity.
24677767	6	10	theme	PECs	727:730	arg1	compositions					707:718	The chemical compositions	694:718	The chemical compositions of the PECs, as well as their influence in the final structure of the scaffolds	694:798	The chemical compositions of the PECs, as well as their influence in the final structure of the scaffolds were meticulously studied.
24677767	11	11	from	increase	1510:1517	arg1	ratios					1539:1544	the CS:CRG molar ratios	1522:1544	the CS:CRG molar ratios	1522:1544	The increase in the CS:CRG molar ratios improved the scaffold mechanical properties, with CSCRG3 presenting the higher compressive modulus under wet conditions.
24677767	5	12	theme	3:1	670:672	arg1	ratios					628:633	CRG molar ratios	618:633	CRG molar ratios of 1:1 (CSCRG1), 2:1 (CSCRG2), and 3:1 (CSCRG3)	618:681	CS:CRG molar ratios of 1:1 (CSCRG1), 2:1 (CSCRG2), and 3:1 (CSCRG3) were used.
24677767	4	13	theme	three-dimensional	473:489	arg1	scaffolds					498:506	three-dimensional porous scaffolds	473:506	three-dimensional porous scaffolds	473:506	In this work, three-dimensional porous scaffolds were produced through the lyophilization of pH sensitive PECs made of chitosan (CS) and carrageenan (CRG).
24677767	8	14	theme	9	1176:1176	arg1	values					1166:1171	nonphysiological values	1149:1171	nonphysiological values of 9 and 11	1149:1183	Results showed that the PECs produced were stable at pH values of 7.4 and under but dissolved as the pH increased to nonphysiological values of 9 and 11.
24677767	7	15	theme	4.5	979:981	arg1	values					916:921	the physiological pH values	895:921	the physiological pH values of 7.4 (simulating normal physiological conditions) and 4.5 (simulating inflammatory response)	895:1016	In addition, the pH responsiveness of the PECs in a range including the physiological pH values of 7.4 (simulating normal physiological conditions) and 4.5 (simulating inflammatory response) was assessed.
24677767	2	16	theme	aqueous	268:274	arg1	medium					276:281	aqueous medium	268:281	aqueous medium	268:281	These substances are obtained in aqueous medium without the need for crosslinking agents.
24677767	8	17	theme	11	1182:1183	arg1	values					1166:1171	nonphysiological values	1149:1171	nonphysiological values of 9 and 11	1149:1183	Results showed that the PECs produced were stable at pH values of 7.4 and under but dissolved as the pH increased to nonphysiological values of 9 and 11.
24677767	11	18	theme	compressive	1625:1635	arg1	modulus					1637:1643	the higher compressive modulus	1614:1643	the higher compressive modulus	1614:1643	The increase in the CS:CRG molar ratios improved the scaffold mechanical properties, with CSCRG3 presenting the higher compressive modulus under wet conditions.
24677767	7	19	theme	physiological	949:961	arg1	conditions					963:972	normal physiological conditions	942:972	normal physiological conditions	942:972	In addition, the pH responsiveness of the PECs in a range including the physiological pH values of 7.4 (simulating normal physiological conditions) and 4.5 (simulating inflammatory response) was assessed.
24677767	7	20	theme	pH	913:914	arg1	values					916:921	the physiological pH values	895:921	the physiological pH values of 7.4 (simulating normal physiological conditions) and 4.5 (simulating inflammatory response)	895:1016	In addition, the pH responsiveness of the PECs in a range including the physiological pH values of 7.4 (simulating normal physiological conditions) and 4.5 (simulating inflammatory response) was assessed.
24677767	1	21	theme	promising	160:168	arg1	materials					170:178	promising materials	160:178	promising materials for drug delivery and tissue engineering applications	160:232	Polyelectrolyte complexes (PECs) represent promising materials for drug delivery and tissue engineering applications.
24677767	0	22	theme	complexes	83:91	arg1	scaffolds					13:21	Novel porous scaffolds	0:21	Novel porous scaffolds of pH responsive chitosan/carrageenan-based polyelectrolyte complexes for tissue engineering.	0:115	Novel porous scaffolds of pH responsive chitosan/carrageenan-based polyelectrolyte complexes for tissue engineering.
24677767	10	23	theme	large	1324:1328	arg1	pores					1349:1353	large and interconnected pores	1324:1353	large and interconnected pores	1324:1353	The scaffolds obtained presented large and interconnected pores, being equally sensitive to changes in the pH. CSCRG1 scaffolds appeared to have higher hydrophilicity and therefore higher water absorption capacity.
24677767	0	24	theme	tissue	97:102	arg1	engineering					104:114	tissue engineering	97:114	tissue engineering	97:114	Novel porous scaffolds of pH responsive chitosan/carrageenan-based polyelectrolyte complexes for tissue engineering.
24677767	5	25	used	used	688:691	arg2	ratios					628:633	CRG molar ratios	618:633	CRG molar ratios of 1:1 (CSCRG1), 2:1 (CSCRG2), and 3:1 (CSCRG3)	618:681	CS:CRG molar ratios of 1:1 (CSCRG1), 2:1 (CSCRG2), and 3:1 (CSCRG3) were used.
24677767	10	26	from	changes	1383:1389	arg1	scaffolds					1409:1417	the pH. CSCRG1 scaffolds	1394:1417	the pH. CSCRG1 scaffolds appeared to have higher hydrophilicity and therefore higher water absorption capacity	1394:1503	The scaffolds obtained presented large and interconnected pores, being equally sensitive to changes in the pH. CSCRG1 scaffolds appeared to have higher hydrophilicity and therefore higher water absorption capacity.
24677767	3	27	theme	grade	400:404	arg1	polymers					406:413	oppositely charged medical grade polymers	373:413	oppositely charged medical grade polymers	373:413	PECs can be produced through the combination of oppositely charged medical grade polymers, which include the stimuli responsive ones.
24677767	4	28	theme	sensitive	555:563	arg1	PECs					565:568	pH sensitive PECs	552:568	pH sensitive PECs made of chitosan (CS) and carrageenan (CRG)	552:612	In this work, three-dimensional porous scaffolds were produced through the lyophilization of pH sensitive PECs made of chitosan (CS) and carrageenan (CRG).
24677767	12	29	theme	physiological	1813:1825	arg1	conditions					1827:1836	physiological conditions	1813:1836	physiological conditions	1813:1836	Overall, the PEC scaffolds appear promising for tissue engineering related applications that require the use of pH responsive materials stable at physiological conditions.
24677767	5	30	theme	CRG	618:620	arg1	ratios					628:633	CRG molar ratios	618:633	CRG molar ratios of 1:1 (CSCRG1), 2:1 (CSCRG2), and 3:1 (CSCRG3)	618:681	CS:CRG molar ratios of 1:1 (CSCRG1), 2:1 (CSCRG2), and 3:1 (CSCRG3) were used.
24677767	3	31	theme	charged	384:390	arg1	polymers					406:413	oppositely charged medical grade polymers	373:413	oppositely charged medical grade polymers	373:413	PECs can be produced through the combination of oppositely charged medical grade polymers, which include the stimuli responsive ones.
24677767	7	32	dep	7.4	926:928	arg1	simulating					931:940	simulating	931:940	simulating normal physiological conditions	931:972	In addition, the pH responsiveness of the PECs in a range including the physiological pH values of 7.4 (simulating normal physiological conditions) and 4.5 (simulating inflammatory response) was assessed.
24677767	10	33	theme	interconnected	1334:1347	arg1	pores					1349:1353	large and interconnected pores	1324:1353	large and interconnected pores	1324:1353	The scaffolds obtained presented large and interconnected pores, being equally sensitive to changes in the pH. CSCRG1 scaffolds appeared to have higher hydrophilicity and therefore higher water absorption capacity.
24677767	0	34	theme	Novel	0:4	arg1	scaffolds					13:21	Novel porous scaffolds	0:21	Novel porous scaffolds of pH responsive chitosan/carrageenan-based polyelectrolyte complexes for tissue engineering.	0:115	Novel porous scaffolds of pH responsive chitosan/carrageenan-based polyelectrolyte complexes for tissue engineering.
24677767	12	35	theme	responsive	1782:1791	arg1	materials					1793:1801	pH responsive materials	1779:1801	pH responsive materials stable at physiological conditions	1779:1836	Overall, the PEC scaffolds appear promising for tissue engineering related applications that require the use of pH responsive materials stable at physiological conditions.
24677767	1	36	theme	Polyelectrolyte	117:131	arg1	PECs					144:147	PECs	144:147	PECs	144:147	Polyelectrolyte complexes (PECs) represent promising materials for drug delivery and tissue engineering applications.
24677767	1	36	theme	Polyelectrolyte	117:131	arg1	complexes					133:141	Polyelectrolyte complexes	117:141	Polyelectrolyte complexes (PECs)	117:148	Polyelectrolyte complexes (PECs) represent promising materials for drug delivery and tissue engineering applications.
24677767	11	37	dep	CS	1526:1527	arg1	CRG					1529:1531	CRG	1529:1531	CRG	1529:1531	The increase in the CS:CRG molar ratios improved the scaffold mechanical properties, with CSCRG3 presenting the higher compressive modulus under wet conditions.
24677767	0	38	theme	pH	26:27	arg1	complexes					83:91	pH responsive chitosan/carrageenan-based polyelectrolyte complexes	26:91	pH responsive chitosan/carrageenan-based polyelectrolyte complexes	26:91	Novel porous scaffolds of pH responsive chitosan/carrageenan-based polyelectrolyte complexes for tissue engineering.
24677767	3	39	theme	stimuli	434:440	arg1	ones					453:456	the stimuli responsive ones	430:456	the stimuli responsive ones	430:456	PECs can be produced through the combination of oppositely charged medical grade polymers, which include the stimuli responsive ones.
24677767	10	40	theme	pH.	1398:1400	arg1	scaffolds					1409:1417	the pH. CSCRG1 scaffolds	1394:1417	the pH. CSCRG1 scaffolds appeared to have higher hydrophilicity and therefore higher water absorption capacity	1394:1503	The scaffolds obtained presented large and interconnected pores, being equally sensitive to changes in the pH. CSCRG1 scaffolds appeared to have higher hydrophilicity and therefore higher water absorption capacity.
24677767	11	41	theme	scaffold	1559:1566	arg1	properties					1579:1588	the scaffold mechanical properties	1555:1588	the scaffold mechanical properties	1555:1588	The increase in the CS:CRG molar ratios improved the scaffold mechanical properties, with CSCRG3 presenting the higher compressive modulus under wet conditions.
24677767	0	42	theme	chitosan/carrageenan-based	40:65	arg1	complexes					83:91	pH responsive chitosan/carrageenan-based polyelectrolyte complexes	26:91	pH responsive chitosan/carrageenan-based polyelectrolyte complexes	26:91	Novel porous scaffolds of pH responsive chitosan/carrageenan-based polyelectrolyte complexes for tissue engineering.
24677767	12	43	theme	tissue	1715:1720	arg1	engineering					1722:1732	tissue engineering	1715:1732	tissue engineering related applications that require the use of pH responsive materials stable at physiological conditions	1715:1836	Overall, the PEC scaffolds appear promising for tissue engineering related applications that require the use of pH responsive materials stable at physiological conditions.
24677767	7	44	theme	pH	844:845	arg1	responsiveness					847:860	the pH responsiveness	840:860	the pH responsiveness of the PECs in a range including the physiological pH values of 7.4 (simulating normal physiological conditions) and 4.5 (simulating inflammatory response)	840:1016	In addition, the pH responsiveness of the PECs in a range including the physiological pH values of 7.4 (simulating normal physiological conditions) and 4.5 (simulating inflammatory response) was assessed.
24677767	12	45	theme	related	1734:1740	arg1	applications					1742:1753	tissue engineering related applications	1715:1753	tissue engineering related applications that require the use of pH responsive materials stable at physiological conditions	1715:1836	Overall, the PEC scaffolds appear promising for tissue engineering related applications that require the use of pH responsive materials stable at physiological conditions.
24677767	6	46	from	compositions	707:718	arg1	structure					773:781	the final structure	763:781	the final structure of the scaffolds	763:798	The chemical compositions of the PECs, as well as their influence in the final structure of the scaffolds were meticulously studied.
24677767	12	47	theme	PEC	1680:1682	arg1	scaffolds					1684:1692	the PEC scaffolds	1676:1692	the PEC scaffolds	1676:1692	Overall, the PEC scaffolds appear promising for tissue engineering related applications that require the use of pH responsive materials stable at physiological conditions.
24677767	12	48	dep	materials	1793:1801	arg1	stable					1803:1808	stable	1803:1808	stable	1803:1808	Overall, the PEC scaffolds appear promising for tissue engineering related applications that require the use of pH responsive materials stable at physiological conditions.
24677767	7	49	theme	inflammatory	995:1006	arg1	response					1008:1015	inflammatory response	995:1015	inflammatory response	995:1015	In addition, the pH responsiveness of the PECs in a range including the physiological pH values of 7.4 (simulating normal physiological conditions) and 4.5 (simulating inflammatory response) was assessed.
24677767	10	50	theme	higher	1436:1441	arg1	hydrophilicity					1443:1456	higher hydrophilicity	1436:1456	higher hydrophilicity	1436:1456	The scaffolds obtained presented large and interconnected pores, being equally sensitive to changes in the pH. CSCRG1 scaffolds appeared to have higher hydrophilicity and therefore higher water absorption capacity.
24677767	10	51	theme	absorption	1485:1494	arg1	capacity					1496:1503	higher water absorption capacity	1472:1503	higher water absorption capacity	1472:1503	The scaffolds obtained presented large and interconnected pores, being equally sensitive to changes in the pH. CSCRG1 scaffolds appeared to have higher hydrophilicity and therefore higher water absorption capacity.
24677767	11	52	theme	molar	1533:1537	arg1	ratios					1539:1544	the CS:CRG molar ratios	1522:1544	the CS:CRG molar ratios	1522:1544	The increase in the CS:CRG molar ratios improved the scaffold mechanical properties, with CSCRG3 presenting the higher compressive modulus under wet conditions.
24677767	5	53	theme	1:1	638:640	arg1	ratios					628:633	CRG molar ratios	618:633	CRG molar ratios of 1:1 (CSCRG1), 2:1 (CSCRG2), and 3:1 (CSCRG3)	618:681	CS:CRG molar ratios of 1:1 (CSCRG1), 2:1 (CSCRG2), and 3:1 (CSCRG3) were used.
24677767	10	54	theme	higher	1472:1477	arg1	absorption					1485:1494	higher water absorption	1472:1494	higher water absorption capacity	1472:1503	The scaffolds obtained presented large and interconnected pores, being equally sensitive to changes in the pH. CSCRG1 scaffolds appeared to have higher hydrophilicity and therefore higher water absorption capacity.
24677767	8	55	theme	7.4	1098:1100	arg1	values					1088:1093	pH values	1085:1093	pH values of 7.4	1085:1100	Results showed that the PECs produced were stable at pH values of 7.4 and under but dissolved as the pH increased to nonphysiological values of 9 and 11.
24677767	4	56	theme	porous	491:496	arg1	scaffolds					498:506	three-dimensional porous scaffolds	473:506	three-dimensional porous scaffolds	473:506	In this work, three-dimensional porous scaffolds were produced through the lyophilization of pH sensitive PECs made of chitosan (CS) and carrageenan (CRG).
24677767	8	57	theme	nonphysiological	1149:1164	arg1	values					1166:1171	nonphysiological values	1149:1171	nonphysiological values of 9 and 11	1149:1183	Results showed that the PECs produced were stable at pH values of 7.4 and under but dissolved as the pH increased to nonphysiological values of 9 and 11.
24677767	6	58	from	structure	773:781	arg1	compositions					707:718	The chemical compositions	694:718	The chemical compositions of the PECs, as well as their influence in the final structure of the scaffolds	694:798	The chemical compositions of the PECs, as well as their influence in the final structure of the scaffolds were meticulously studied.
24677767	10	59	dep	scaffolds	1409:1417	arg1	appeared					1419:1426	appeared	1419:1426	appeared to have higher hydrophilicity	1419:1456	The scaffolds obtained presented large and interconnected pores, being equally sensitive to changes in the pH. CSCRG1 scaffolds appeared to have higher hydrophilicity and therefore higher water absorption capacity.
24677767	10	59	dep	scaffolds	1409:1417	arg1	capacity					1496:1503	higher water absorption capacity	1472:1503	higher water absorption capacity	1472:1503	The scaffolds obtained presented large and interconnected pores, being equally sensitive to changes in the pH. CSCRG1 scaffolds appeared to have higher hydrophilicity and therefore higher water absorption capacity.
24677767	7	60	theme	normal	942:947	arg1	conditions					963:972	normal physiological conditions	942:972	normal physiological conditions	942:972	In addition, the pH responsiveness of the PECs in a range including the physiological pH values of 7.4 (simulating normal physiological conditions) and 4.5 (simulating inflammatory response) was assessed.
24677767	7	61	theme	7.4	926:928	arg1	values					916:921	the physiological pH values	895:921	the physiological pH values of 7.4 (simulating normal physiological conditions) and 4.5 (simulating inflammatory response)	895:1016	In addition, the pH responsiveness of the PECs in a range including the physiological pH values of 7.4 (simulating normal physiological conditions) and 4.5 (simulating inflammatory response) was assessed.
24677767	12	62	from	conditions	1827:1836	arg1	stable					1803:1808	stable	1803:1808	stable	1803:1808	Overall, the PEC scaffolds appear promising for tissue engineering related applications that require the use of pH responsive materials stable at physiological conditions.
24677767	11	63	theme	higher	1618:1623	arg1	modulus					1637:1643	the higher compressive modulus	1614:1643	the higher compressive modulus	1614:1643	The increase in the CS:CRG molar ratios improved the scaffold mechanical properties, with CSCRG3 presenting the higher compressive modulus under wet conditions.
24677767	7	64	theme	physiological	899:911	arg1	values					916:921	the physiological pH values	895:921	the physiological pH values of 7.4 (simulating normal physiological conditions) and 4.5 (simulating inflammatory response)	895:1016	In addition, the pH responsiveness of the PECs in a range including the physiological pH values of 7.4 (simulating normal physiological conditions) and 4.5 (simulating inflammatory response) was assessed.
24677767	1	65	theme	drug	184:187	arg1	delivery					189:196	drug delivery	184:196	drug delivery	184:196	Polyelectrolyte complexes (PECs) represent promising materials for drug delivery and tissue engineering applications.
24677767	4	66	theme	PECs	565:568	arg1	lyophilization					534:547	the lyophilization	530:547	the lyophilization of pH sensitive PECs made of chitosan (CS) and carrageenan (CRG)	530:612	In this work, three-dimensional porous scaffolds were produced through the lyophilization of pH sensitive PECs made of chitosan (CS) and carrageenan (CRG).
24677767	3	67	theme	polymers	406:413	arg1	combination					358:368	the combination	354:368	the combination of oppositely charged medical grade polymers, which include the stimuli responsive ones	354:456	PECs can be produced through the combination of oppositely charged medical grade polymers, which include the stimuli responsive ones.
24677767	11	68	theme	wet	1651:1653	arg1	conditions					1655:1664	wet conditions	1651:1664	wet conditions	1651:1664	The increase in the CS:CRG molar ratios improved the scaffold mechanical properties, with CSCRG3 presenting the higher compressive modulus under wet conditions.
24677767	12	69	theme	pH	1779:1780	arg1	materials					1793:1801	pH responsive materials	1779:1801	pH responsive materials stable at physiological conditions	1779:1836	Overall, the PEC scaffolds appear promising for tissue engineering related applications that require the use of pH responsive materials stable at physiological conditions.
24677767	5	70	theme	molar	622:626	arg1	ratios					628:633	CRG molar ratios	618:633	CRG molar ratios of 1:1 (CSCRG1), 2:1 (CSCRG2), and 3:1 (CSCRG3)	618:681	CS:CRG molar ratios of 1:1 (CSCRG1), 2:1 (CSCRG2), and 3:1 (CSCRG3) were used.
24677767	3	71	theme	medical	392:398	arg1	polymers					406:413	oppositely charged medical grade polymers	373:413	oppositely charged medical grade polymers	373:413	PECs can be produced through the combination of oppositely charged medical grade polymers, which include the stimuli responsive ones.
24677767	0	72	theme	porous	6:11	arg1	scaffolds					13:21	Novel porous scaffolds	0:21	Novel porous scaffolds of pH responsive chitosan/carrageenan-based polyelectrolyte complexes for tissue engineering.	0:115	Novel porous scaffolds of pH responsive chitosan/carrageenan-based polyelectrolyte complexes for tissue engineering.
24677767	12	73	theme	materials	1793:1801	arg1	use					1772:1774	the use	1768:1774	the use of pH responsive materials stable at physiological conditions	1768:1836	Overall, the PEC scaffolds appear promising for tissue engineering related applications that require the use of pH responsive materials stable at physiological conditions.
24677767	7	74	theme	PECs	869:872	arg1	responsiveness					847:860	the pH responsiveness	840:860	the pH responsiveness of the PECs in a range including the physiological pH values of 7.4 (simulating normal physiological conditions) and 4.5 (simulating inflammatory response)	840:1016	In addition, the pH responsiveness of the PECs in a range including the physiological pH values of 7.4 (simulating normal physiological conditions) and 4.5 (simulating inflammatory response) was assessed.
24677767	6	75	from	influence	750:758	arg1	structure					773:781	the final structure	763:781	the final structure of the scaffolds	763:798	The chemical compositions of the PECs, as well as their influence in the final structure of the scaffolds were meticulously studied.
24677767	1	76	dep	delivery	189:196	arg1	applications					221:232	applications	221:232	applications	221:232	Polyelectrolyte complexes (PECs) represent promising materials for drug delivery and tissue engineering applications.
24677767	6	77	from	PECs	727:730	arg1	structure					773:781	the final structure	763:781	the final structure of the scaffolds	763:798	The chemical compositions of the PECs, as well as their influence in the final structure of the scaffolds were meticulously studied.
24677767	11	78	theme	mechanical	1568:1577	arg1	properties					1579:1588	the scaffold mechanical properties	1555:1588	the scaffold mechanical properties	1555:1588	The increase in the CS:CRG molar ratios improved the scaffold mechanical properties, with CSCRG3 presenting the higher compressive modulus under wet conditions.
24677767	0	79	theme	responsive	29:38	arg1	complexes					83:91	pH responsive chitosan/carrageenan-based polyelectrolyte complexes	26:91	pH responsive chitosan/carrageenan-based polyelectrolyte complexes	26:91	Novel porous scaffolds of pH responsive chitosan/carrageenan-based polyelectrolyte complexes for tissue engineering.
24677767	6	80	theme	scaffolds	790:798	arg1	structure					773:781	the final structure	763:781	the final structure of the scaffolds	763:798	The chemical compositions of the PECs, as well as their influence in the final structure of the scaffolds were meticulously studied.
24677767	0	81	theme	polyelectrolyte	67:81	arg1	complexes					83:91	pH responsive chitosan/carrageenan-based polyelectrolyte complexes	26:91	pH responsive chitosan/carrageenan-based polyelectrolyte complexes	26:91	Novel porous scaffolds of pH responsive chitosan/carrageenan-based polyelectrolyte complexes for tissue engineering.
24677767	12	82	theme	engineering	1722:1732	arg1	applications					1742:1753	tissue engineering related applications	1715:1753	tissue engineering related applications that require the use of pH responsive materials stable at physiological conditions	1715:1836	Overall, the PEC scaffolds appear promising for tissue engineering related applications that require the use of pH responsive materials stable at physiological conditions.
24677767	3	83	theme	responsive	442:451	arg1	ones					453:456	the stimuli responsive ones	430:456	the stimuli responsive ones	430:456	PECs can be produced through the combination of oppositely charged medical grade polymers, which include the stimuli responsive ones.
24677767	6	84	theme	chemical	698:705	arg1	compositions					707:718	The chemical compositions	694:718	The chemical compositions of the PECs, as well as their influence in the final structure of the scaffolds	694:798	The chemical compositions of the PECs, as well as their influence in the final structure of the scaffolds were meticulously studied.
24677767	6	85	theme	final	767:771	arg1	structure					773:781	the final structure	763:781	the final structure of the scaffolds	763:798	The chemical compositions of the PECs, as well as their influence in the final structure of the scaffolds were meticulously studied.
24677767	7	86	dep	4.5	979:981	arg1	simulating					984:993	simulating	984:993	simulating inflammatory response	984:1015	In addition, the pH responsiveness of the PECs in a range including the physiological pH values of 7.4 (simulating normal physiological conditions) and 4.5 (simulating inflammatory response) was assessed.
24677767	11	87	theme	CS	1526:1527	arg1	ratios					1539:1544	the CS:CRG molar ratios	1522:1544	the CS:CRG molar ratios	1522:1544	The increase in the CS:CRG molar ratios improved the scaffold mechanical properties, with CSCRG3 presenting the higher compressive modulus under wet conditions.
24998355	7	0	theme	maximal	1160:1166	arg1	production					1172:1181	maximal CGC production	1160:1181	maximal CGC production	1160:1181	Therefore, a compromise between maximal CGC production and the synthesis of polymers enriched in chitin must be achieved, depending on the final application of this product.
24998355	6	1	dep	mol	1089:1091	arg1	%					1088:1088	%	1088:1088	%	1088:1088	The highest chitin:β-glucan molar ratio (>14:86) was obtained for the mid-range pH (4.5-5.8) and temperatures (26-33°C), while a drastic reduction of chitin to ≤ 6%mol was observed outside those ranges.
24998355	1	2	theme	Chitin-glucan	145:157	arg1	biomaterial					187:197	a valuable biomaterial	176:197	a valuable biomaterial that can be extracted from the cell wall of several yeast and fungi	176:265	Chitin-glucan complex (CGC) is a valuable biomaterial that can be extracted from the cell wall of several yeast and fungi.
24998355	1	2	theme	Chitin-glucan	145:157	arg1	CGC					168:170	CGC	168:170	CGC	168:170	Chitin-glucan complex (CGC) is a valuable biomaterial that can be extracted from the cell wall of several yeast and fungi.
24998355	1	2	theme	Chitin-glucan	145:157	arg1	complex					159:165	Chitin-glucan complex	145:165	Chitin-glucan complex (CGC)	145:171	Chitin-glucan complex (CGC) is a valuable biomaterial that can be extracted from the cell wall of several yeast and fungi.
24998355	5	3	theme	molar	892:896	arg1	ratio					898:902	the polymer's chitin:β-glucan molar ratio	862:902	the polymer's chitin:β-glucan molar ratio	862:902	In contrast, the effect of pH and temperature on the polymer's chitin:β-glucan molar ratio was more pronounced.
24998355	7	4	theme	polymers	1204:1211	arg1	synthesis					1191:1199	the synthesis	1187:1199	the synthesis of polymers enriched in chitin	1187:1230	Therefore, a compromise between maximal CGC production and the synthesis of polymers enriched in chitin must be achieved, depending on the final application of this product.
24998355	7	4	theme	polymers	1204:1211	arg1	production					1172:1181	maximal CGC production	1160:1181	maximal CGC production	1160:1181	Therefore, a compromise between maximal CGC production and the synthesis of polymers enriched in chitin must be achieved, depending on the final application of this product.
24998355	6	5	dep	%	1088:1088	arg1	6					1087:1087	6	1087:1087	6	1087:1087	The highest chitin:β-glucan molar ratio (>14:86) was obtained for the mid-range pH (4.5-5.8) and temperatures (26-33°C), while a drastic reduction of chitin to ≤ 6%mol was observed outside those ranges.
24998355	1	6	theme	cell	230:233	arg1	wall					235:238	the cell wall	226:238	the cell wall of several yeast and fungi	226:265	Chitin-glucan complex (CGC) is a valuable biomaterial that can be extracted from the cell wall of several yeast and fungi.
24998355	2	7	theme	temperature	447:457	arg1	effect					420:425	the effect	416:425	the effect of pH (3.5-6.5) and temperature (20-40°C) on CGC production and polymer composition	416:509	In this work, the yeast Komagataella (Pichia) pastoris was grown on glycerol as the sole carbon source in batch cultivation experiments to evaluate the effect of pH (3.5-6.5) and temperature (20-40°C) on CGC production and polymer composition.
24998355	5	8	theme	pH	840:841	arg1	pronounced					913:922	pronounced	913:922	pronounced	913:922	In contrast, the effect of pH and temperature on the polymer's chitin:β-glucan molar ratio was more pronounced.
24998355	5	8	theme	pH	840:841	arg1	effect					830:835	the effect	826:835	the effect of pH and temperature on the polymer's chitin:β-glucan molar ratio	826:902	In contrast, the effect of pH and temperature on the polymer's chitin:β-glucan molar ratio was more pronounced.
24998355	7	9	theme	CGC	1168:1170	arg1	production					1172:1181	maximal CGC production	1160:1181	maximal CGC production	1160:1181	Therefore, a compromise between maximal CGC production and the synthesis of polymers enriched in chitin must be achieved, depending on the final application of this product.
24998355	0	10	theme	cultivation	78:88	arg1	impact					68:73	impact	68:73	Chitin-glucan complex production by Komagataella (Pichia) pastoris: impact of cultivation pH and temperature on polymer content and composition.	0:143	Chitin-glucan complex production by Komagataella (Pichia) pastoris: impact of cultivation pH and temperature on polymer content and composition.
24998355	3	11	theme	pH	630:631	arg1	ranges					649:654	the tested pH and temperature ranges	619:654	the tested pH and temperature ranges	619:654	The CGC content in the biomass and the volumetric productivity (rp) were not significantly affected within the tested pH and temperature ranges.
24998355	2	12	theme	Pichia	306:311	arg1	source					364:369	the sole carbon source	348:369	the sole carbon source	348:369	In this work, the yeast Komagataella (Pichia) pastoris was grown on glycerol as the sole carbon source in batch cultivation experiments to evaluate the effect of pH (3.5-6.5) and temperature (20-40°C) on CGC production and polymer composition.
24998355	2	12	theme	Pichia	306:311	arg1	pastoris					314:321	the yeast Komagataella (Pichia) pastoris	282:321	the yeast Komagataella (Pichia) pastoris	282:321	In this work, the yeast Komagataella (Pichia) pastoris was grown on glycerol as the sole carbon source in batch cultivation experiments to evaluate the effect of pH (3.5-6.5) and temperature (20-40°C) on CGC production and polymer composition.
24998355	3	13	from	content	520:526	arg1	biomass					535:541	the biomass	531:541	the biomass	531:541	The CGC content in the biomass and the volumetric productivity (rp) were not significantly affected within the tested pH and temperature ranges.
24998355	6	14	theme	highest	929:935	arg1	chitin					937:942	The highest chitin	925:942	The highest chitin:β-glucan molar ratio (>14:86) was obtained for the mid-range pH (4.5-5.8) and temperatures (26-33°C), while a drastic reduction of chitin to ≤ 6%mol was observed outside those ranges.	925:1126	The highest chitin:β-glucan molar ratio (>14:86) was obtained for the mid-range pH (4.5-5.8) and temperatures (26-33°C), while a drastic reduction of chitin to ≤ 6%mol was observed outside those ranges.
24998355	2	15	theme	sole	352:355	arg1	source					364:369	the sole carbon source	348:369	the sole carbon source	348:369	In this work, the yeast Komagataella (Pichia) pastoris was grown on glycerol as the sole carbon source in batch cultivation experiments to evaluate the effect of pH (3.5-6.5) and temperature (20-40°C) on CGC production and polymer composition.
24998355	2	15	theme	sole	352:355	arg1	pastoris					314:321	the yeast Komagataella (Pichia) pastoris	282:321	the yeast Komagataella (Pichia) pastoris	282:321	In this work, the yeast Komagataella (Pichia) pastoris was grown on glycerol as the sole carbon source in batch cultivation experiments to evaluate the effect of pH (3.5-6.5) and temperature (20-40°C) on CGC production and polymer composition.
24998355	3	16	from	productivity	562:573	arg1	biomass					535:541	the biomass	531:541	the biomass	531:541	The CGC content in the biomass and the volumetric productivity (rp) were not significantly affected within the tested pH and temperature ranges.
24998355	4	17	theme	≥	725:725	arg1	-1					738:739	-1	738:739	-1	738:739	Nevertheless, both parameters could be maximized (CGC ≥14wt% and rp ≥ 3.0 gCGC L(-1)day(-1)) for temperatures within 27-34°C and pH above 6.0 or below 4.0.
24998355	4	17	theme	≥	725:725	arg1	L					736:736	rp ≥ 3.0 gCGC L	722:736	rp ≥ 3.0 gCGC L(-1)	722:740	Nevertheless, both parameters could be maximized (CGC ≥14wt% and rp ≥ 3.0 gCGC L(-1)day(-1)) for temperatures within 27-34°C and pH above 6.0 or below 4.0.
24998355	0	18	dep	cultivation	78:88	arg1	pH					90:91	pH	90:91	pH	90:91	Chitin-glucan complex production by Komagataella (Pichia) pastoris: impact of cultivation pH and temperature on polymer content and composition.
24998355	0	18	dep	cultivation	78:88	arg1	temperature					97:107	temperature	97:107	temperature	97:107	Chitin-glucan complex production by Komagataella (Pichia) pastoris: impact of cultivation pH and temperature on polymer content and composition.
24998355	6	19	dep	chitin	937:942	arg1	obtained					978:985	obtained	978:985	obtained for the mid-range pH (4.5-5.8) and temperatures (26-33°C)	978:1043	The highest chitin:β-glucan molar ratio (>14:86) was obtained for the mid-range pH (4.5-5.8) and temperatures (26-33°C), while a drastic reduction of chitin to ≤ 6%mol was observed outside those ranges.
24998355	0	20	theme	complex	14:20	arg1	production					22:31	Chitin-glucan complex production	0:31	Chitin-glucan complex production by Komagataella (Pichia) pastoris: impact of cultivation pH and temperature on polymer content and composition.	0:143	Chitin-glucan complex production by Komagataella (Pichia) pastoris: impact of cultivation pH and temperature on polymer content and composition.
24998355	3	21	theme	tested	623:628	arg1	ranges					649:654	the tested pH and temperature ranges	619:654	the tested pH and temperature ranges	619:654	The CGC content in the biomass and the volumetric productivity (rp) were not significantly affected within the tested pH and temperature ranges.
24998355	6	22	theme	molar	953:957	arg1	>14:86					966:971	>14:86	966:971	>14:86	966:971	The highest chitin:β-glucan molar ratio (>14:86) was obtained for the mid-range pH (4.5-5.8) and temperatures (26-33°C), while a drastic reduction of chitin to ≤ 6%mol was observed outside those ranges.
24998355	6	22	theme	molar	953:957	arg1	ratio					959:963	β-glucan molar ratio	944:963	β-glucan molar ratio (>14:86)	944:972	The highest chitin:β-glucan molar ratio (>14:86) was obtained for the mid-range pH (4.5-5.8) and temperatures (26-33°C), while a drastic reduction of chitin to ≤ 6%mol was observed outside those ranges.
24998355	5	23	from	contrast	816:823	arg1	effect					830:835	the effect	826:835	the effect of pH and temperature on the polymer's chitin:β-glucan molar ratio	826:902	In contrast, the effect of pH and temperature on the polymer's chitin:β-glucan molar ratio was more pronounced.
24998355	5	23	from	contrast	816:823	arg1	pronounced					913:922	pronounced	913:922	pronounced	913:922	In contrast, the effect of pH and temperature on the polymer's chitin:β-glucan molar ratio was more pronounced.
24998355	4	24	dep	maximized	696:704	arg1	%					716:716	CGC ≥14wt%	707:716	CGC ≥14wt%	707:716	Nevertheless, both parameters could be maximized (CGC ≥14wt% and rp ≥ 3.0 gCGC L(-1)day(-1)) for temperatures within 27-34°C and pH above 6.0 or below 4.0.
24998355	4	24	dep	maximized	696:704	arg1	L					736:736	rp ≥ 3.0 gCGC L	722:736	rp ≥ 3.0 gCGC L(-1)	722:740	Nevertheless, both parameters could be maximized (CGC ≥14wt% and rp ≥ 3.0 gCGC L(-1)day(-1)) for temperatures within 27-34°C and pH above 6.0 or below 4.0.
24998355	4	24	dep	maximized	696:704	arg1	-1					738:739	-1	738:739	-1	738:739	Nevertheless, both parameters could be maximized (CGC ≥14wt% and rp ≥ 3.0 gCGC L(-1)day(-1)) for temperatures within 27-34°C and pH above 6.0 or below 4.0.
24998355	0	25	theme	Chitin-glucan	0:12	arg1	production					22:31	Chitin-glucan complex production	0:31	Chitin-glucan complex production by Komagataella (Pichia) pastoris: impact of cultivation pH and temperature on polymer content and composition.	0:143	Chitin-glucan complex production by Komagataella (Pichia) pastoris: impact of cultivation pH and temperature on polymer content and composition.
24998355	1	26	theme	several	243:249	arg1	yeast					251:255	several yeast	243:255	several yeast	243:255	Chitin-glucan complex (CGC) is a valuable biomaterial that can be extracted from the cell wall of several yeast and fungi.
24998355	4	27	theme	gCGC	731:734	arg1	-1					738:739	-1	738:739	-1	738:739	Nevertheless, both parameters could be maximized (CGC ≥14wt% and rp ≥ 3.0 gCGC L(-1)day(-1)) for temperatures within 27-34°C and pH above 6.0 or below 4.0.
24998355	4	27	theme	gCGC	731:734	arg1	L					736:736	rp ≥ 3.0 gCGC L	722:736	rp ≥ 3.0 gCGC L(-1)	722:740	Nevertheless, both parameters could be maximized (CGC ≥14wt% and rp ≥ 3.0 gCGC L(-1)day(-1)) for temperatures within 27-34°C and pH above 6.0 or below 4.0.
24998355	7	28	theme	product	1293:1299	arg1	application					1273:1283	the final application	1263:1283	the final application of this product	1263:1299	Therefore, a compromise between maximal CGC production and the synthesis of polymers enriched in chitin must be achieved, depending on the final application of this product.
24998355	6	29	theme	β-glucan	944:951	arg1	>14:86					966:971	>14:86	966:971	>14:86	966:971	The highest chitin:β-glucan molar ratio (>14:86) was obtained for the mid-range pH (4.5-5.8) and temperatures (26-33°C), while a drastic reduction of chitin to ≤ 6%mol was observed outside those ranges.
24998355	6	29	theme	β-glucan	944:951	arg1	ratio					959:963	β-glucan molar ratio	944:963	β-glucan molar ratio (>14:86)	944:972	The highest chitin:β-glucan molar ratio (>14:86) was obtained for the mid-range pH (4.5-5.8) and temperatures (26-33°C), while a drastic reduction of chitin to ≤ 6%mol was observed outside those ranges.
24998355	2	30	from	effect	420:425	arg1	production					476:485	CGC production	472:485	CGC production	472:485	In this work, the yeast Komagataella (Pichia) pastoris was grown on glycerol as the sole carbon source in batch cultivation experiments to evaluate the effect of pH (3.5-6.5) and temperature (20-40°C) on CGC production and polymer composition.
24998355	2	30	from	effect	420:425	arg1	composition					499:509	polymer composition	491:509	polymer composition	491:509	In this work, the yeast Komagataella (Pichia) pastoris was grown on glycerol as the sole carbon source in batch cultivation experiments to evaluate the effect of pH (3.5-6.5) and temperature (20-40°C) on CGC production and polymer composition.
24998355	1	31	theme	yeast	251:255	arg1	wall					235:238	the cell wall	226:238	the cell wall of several yeast and fungi	226:265	Chitin-glucan complex (CGC) is a valuable biomaterial that can be extracted from the cell wall of several yeast and fungi.
24998355	0	32	theme	polymer	112:118	arg1	content					120:126	polymer content	112:126	polymer content	112:126	Chitin-glucan complex production by Komagataella (Pichia) pastoris: impact of cultivation pH and temperature on polymer content and composition.
24998355	2	33	theme	carbon	357:362	arg1	source					364:369	the sole carbon source	348:369	the sole carbon source	348:369	In this work, the yeast Komagataella (Pichia) pastoris was grown on glycerol as the sole carbon source in batch cultivation experiments to evaluate the effect of pH (3.5-6.5) and temperature (20-40°C) on CGC production and polymer composition.
24998355	2	33	theme	carbon	357:362	arg1	pastoris					314:321	the yeast Komagataella (Pichia) pastoris	282:321	the yeast Komagataella (Pichia) pastoris	282:321	In this work, the yeast Komagataella (Pichia) pastoris was grown on glycerol as the sole carbon source in batch cultivation experiments to evaluate the effect of pH (3.5-6.5) and temperature (20-40°C) on CGC production and polymer composition.
24998355	3	34	theme	temperature	637:647	arg1	ranges					649:654	the tested pH and temperature ranges	619:654	the tested pH and temperature ranges	619:654	The CGC content in the biomass and the volumetric productivity (rp) were not significantly affected within the tested pH and temperature ranges.
24998355	2	35	theme	batch	374:378	arg1	experiments					392:402	batch cultivation experiments	374:402	batch cultivation experiments	374:402	In this work, the yeast Komagataella (Pichia) pastoris was grown on glycerol as the sole carbon source in batch cultivation experiments to evaluate the effect of pH (3.5-6.5) and temperature (20-40°C) on CGC production and polymer composition.
24998355	3	36	theme	volumetric	551:560	arg1	rp					576:577	rp	576:577	rp	576:577	The CGC content in the biomass and the volumetric productivity (rp) were not significantly affected within the tested pH and temperature ranges.
24998355	3	36	theme	volumetric	551:560	arg1	productivity					562:573	the volumetric productivity	547:573	the volumetric productivity (rp)	547:578	The CGC content in the biomass and the volumetric productivity (rp) were not significantly affected within the tested pH and temperature ranges.
24998355	5	37	from	effect	830:835	arg1	ratio					898:902	the polymer's chitin:β-glucan molar ratio	862:902	the polymer's chitin:β-glucan molar ratio	862:902	In contrast, the effect of pH and temperature on the polymer's chitin:β-glucan molar ratio was more pronounced.
24998355	1	38	theme	valuable	178:185	arg1	biomaterial					187:197	a valuable biomaterial	176:197	a valuable biomaterial that can be extracted from the cell wall of several yeast and fungi	176:265	Chitin-glucan complex (CGC) is a valuable biomaterial that can be extracted from the cell wall of several yeast and fungi.
24998355	1	38	theme	valuable	178:185	arg1	complex					159:165	Chitin-glucan complex	145:165	Chitin-glucan complex (CGC)	145:171	Chitin-glucan complex (CGC) is a valuable biomaterial that can be extracted from the cell wall of several yeast and fungi.
24998355	1	39	theme	fungi	261:265	arg1	wall					235:238	the cell wall	226:238	the cell wall of several yeast and fungi	226:265	Chitin-glucan complex (CGC) is a valuable biomaterial that can be extracted from the cell wall of several yeast and fungi.
24998355	0	40	from	impact	68:73	arg1	content					120:126	polymer content	112:126	polymer content	112:126	Chitin-glucan complex production by Komagataella (Pichia) pastoris: impact of cultivation pH and temperature on polymer content and composition.
24998355	0	40	from	impact	68:73	arg1	composition					132:142	composition	132:142	composition	132:142	Chitin-glucan complex production by Komagataella (Pichia) pastoris: impact of cultivation pH and temperature on polymer content and composition.
24998355	4	41	dep	%	716:716	arg1	day					741:743	day(-1)	741:747	day(-1)	741:747	Nevertheless, both parameters could be maximized (CGC ≥14wt% and rp ≥ 3.0 gCGC L(-1)day(-1)) for temperatures within 27-34°C and pH above 6.0 or below 4.0.
24998355	0	42	theme	Komagataella	36:47	arg1	pastoris					58:65	Komagataella (Pichia) pastoris	36:65	Komagataella (Pichia) pastoris	36:65	Chitin-glucan complex production by Komagataella (Pichia) pastoris: impact of cultivation pH and temperature on polymer content and composition.
24998355	6	43	theme	chitin	1075:1080	arg1	reduction					1062:1070	a drastic reduction	1052:1070	a drastic reduction of chitin to ≤ 6%mol	1052:1091	The highest chitin:β-glucan molar ratio (>14:86) was obtained for the mid-range pH (4.5-5.8) and temperatures (26-33°C), while a drastic reduction of chitin to ≤ 6%mol was observed outside those ranges.
24998355	3	44	theme	CGC	516:518	arg1	content					520:526	The CGC content	512:526	The CGC content in the biomass	512:541	The CGC content in the biomass and the volumetric productivity (rp) were not significantly affected within the tested pH and temperature ranges.
24998355	5	45	from	pronounced	913:922	arg1	contrast					816:823	contrast	816:823	contrast	816:823	In contrast, the effect of pH and temperature on the polymer's chitin:β-glucan molar ratio was more pronounced.
24998355	7	46	dep	a	1139:1139	arg1	compromise					1141:1150	compromise	1141:1150	compromise	1141:1150	Therefore, a compromise between maximal CGC production and the synthesis of polymers enriched in chitin must be achieved, depending on the final application of this product.
24998355	2	47	theme	polymer	491:497	arg1	composition					499:509	polymer composition	491:509	polymer composition	491:509	In this work, the yeast Komagataella (Pichia) pastoris was grown on glycerol as the sole carbon source in batch cultivation experiments to evaluate the effect of pH (3.5-6.5) and temperature (20-40°C) on CGC production and polymer composition.
24998355	5	48	theme	β-glucan	883:890	arg1	ratio					898:902	the polymer's chitin:β-glucan molar ratio	862:902	the polymer's chitin:β-glucan molar ratio	862:902	In contrast, the effect of pH and temperature on the polymer's chitin:β-glucan molar ratio was more pronounced.
24998355	4	49	theme	rp	722:723	arg1	-1					738:739	-1	738:739	-1	738:739	Nevertheless, both parameters could be maximized (CGC ≥14wt% and rp ≥ 3.0 gCGC L(-1)day(-1)) for temperatures within 27-34°C and pH above 6.0 or below 4.0.
24998355	4	49	theme	rp	722:723	arg1	L					736:736	rp ≥ 3.0 gCGC L	722:736	rp ≥ 3.0 gCGC L(-1)	722:740	Nevertheless, both parameters could be maximized (CGC ≥14wt% and rp ≥ 3.0 gCGC L(-1)day(-1)) for temperatures within 27-34°C and pH above 6.0 or below 4.0.
24998355	2	50	theme	Komagataella	292:303	arg1	source					364:369	the sole carbon source	348:369	the sole carbon source	348:369	In this work, the yeast Komagataella (Pichia) pastoris was grown on glycerol as the sole carbon source in batch cultivation experiments to evaluate the effect of pH (3.5-6.5) and temperature (20-40°C) on CGC production and polymer composition.
24998355	2	50	theme	Komagataella	292:303	arg1	pastoris					314:321	the yeast Komagataella (Pichia) pastoris	282:321	the yeast Komagataella (Pichia) pastoris	282:321	In this work, the yeast Komagataella (Pichia) pastoris was grown on glycerol as the sole carbon source in batch cultivation experiments to evaluate the effect of pH (3.5-6.5) and temperature (20-40°C) on CGC production and polymer composition.
24998355	0	51	theme	Pichia	50:55	arg1	pastoris					58:65	Komagataella (Pichia) pastoris	36:65	Komagataella (Pichia) pastoris	36:65	Chitin-glucan complex production by Komagataella (Pichia) pastoris: impact of cultivation pH and temperature on polymer content and composition.
24998355	5	52	theme	temperature	847:857	arg1	pronounced					913:922	pronounced	913:922	pronounced	913:922	In contrast, the effect of pH and temperature on the polymer's chitin:β-glucan molar ratio was more pronounced.
24998355	5	52	theme	temperature	847:857	arg1	effect					830:835	the effect	826:835	the effect of pH and temperature on the polymer's chitin:β-glucan molar ratio	826:902	In contrast, the effect of pH and temperature on the polymer's chitin:β-glucan molar ratio was more pronounced.
24998355	6	53	theme	mid-range	995:1003	arg1	pH					1005:1006	the mid-range pH	991:1006	the mid-range pH (4.5-5.8)	991:1016	The highest chitin:β-glucan molar ratio (>14:86) was obtained for the mid-range pH (4.5-5.8) and temperatures (26-33°C), while a drastic reduction of chitin to ≤ 6%mol was observed outside those ranges.
24998355	6	53	theme	mid-range	995:1003	arg1	4.5-5.8					1009:1015	4.5-5.8	1009:1015	4.5-5.8	1009:1015	The highest chitin:β-glucan molar ratio (>14:86) was obtained for the mid-range pH (4.5-5.8) and temperatures (26-33°C), while a drastic reduction of chitin to ≤ 6%mol was observed outside those ranges.
24998355	2	54	theme	yeast	286:290	arg1	source					364:369	the sole carbon source	348:369	the sole carbon source	348:369	In this work, the yeast Komagataella (Pichia) pastoris was grown on glycerol as the sole carbon source in batch cultivation experiments to evaluate the effect of pH (3.5-6.5) and temperature (20-40°C) on CGC production and polymer composition.
24998355	2	54	theme	yeast	286:290	arg1	pastoris					314:321	the yeast Komagataella (Pichia) pastoris	282:321	the yeast Komagataella (Pichia) pastoris	282:321	In this work, the yeast Komagataella (Pichia) pastoris was grown on glycerol as the sole carbon source in batch cultivation experiments to evaluate the effect of pH (3.5-6.5) and temperature (20-40°C) on CGC production and polymer composition.
24998355	7	55	theme	final	1267:1271	arg1	application					1273:1283	the final application	1263:1283	the final application of this product	1263:1299	Therefore, a compromise between maximal CGC production and the synthesis of polymers enriched in chitin must be achieved, depending on the final application of this product.
24998355	6	56	theme	drastic	1054:1060	arg1	reduction					1062:1070	a drastic reduction	1052:1070	a drastic reduction of chitin to ≤ 6%mol	1052:1091	The highest chitin:β-glucan molar ratio (>14:86) was obtained for the mid-range pH (4.5-5.8) and temperatures (26-33°C), while a drastic reduction of chitin to ≤ 6%mol was observed outside those ranges.
24998355	4	57	theme	≥14wt	711:715	arg1	%					716:716	CGC ≥14wt%	707:716	CGC ≥14wt%	707:716	Nevertheless, both parameters could be maximized (CGC ≥14wt% and rp ≥ 3.0 gCGC L(-1)day(-1)) for temperatures within 27-34°C and pH above 6.0 or below 4.0.
24998355	2	58	theme	CGC	472:474	arg1	production					476:485	CGC production	472:485	CGC production	472:485	In this work, the yeast Komagataella (Pichia) pastoris was grown on glycerol as the sole carbon source in batch cultivation experiments to evaluate the effect of pH (3.5-6.5) and temperature (20-40°C) on CGC production and polymer composition.
24998355	0	59	dep	production	22:31	arg1	impact					68:73	impact	68:73	Chitin-glucan complex production by Komagataella (Pichia) pastoris: impact of cultivation pH and temperature on polymer content and composition.	0:143	Chitin-glucan complex production by Komagataella (Pichia) pastoris: impact of cultivation pH and temperature on polymer content and composition.
24998355	5	60	theme	chitin	876:881	arg1	ratio					898:902	the polymer's chitin:β-glucan molar ratio	862:902	the polymer's chitin:β-glucan molar ratio	862:902	In contrast, the effect of pH and temperature on the polymer's chitin:β-glucan molar ratio was more pronounced.
24998355	4	61	theme	CGC	707:709	arg1	%					716:716	CGC ≥14wt%	707:716	CGC ≥14wt%	707:716	Nevertheless, both parameters could be maximized (CGC ≥14wt% and rp ≥ 3.0 gCGC L(-1)day(-1)) for temperatures within 27-34°C and pH above 6.0 or below 4.0.
24998355	2	62	theme	pH	430:431	arg1	effect					420:425	the effect	416:425	the effect of pH (3.5-6.5) and temperature (20-40°C) on CGC production and polymer composition	416:509	In this work, the yeast Komagataella (Pichia) pastoris was grown on glycerol as the sole carbon source in batch cultivation experiments to evaluate the effect of pH (3.5-6.5) and temperature (20-40°C) on CGC production and polymer composition.
24998355	2	63	theme	cultivation	380:390	arg1	experiments					392:402	batch cultivation experiments	374:402	batch cultivation experiments	374:402	In this work, the yeast Komagataella (Pichia) pastoris was grown on glycerol as the sole carbon source in batch cultivation experiments to evaluate the effect of pH (3.5-6.5) and temperature (20-40°C) on CGC production and polymer composition.
28482594	4	0	theme	neurons	769:775	arg1	number					749:754	the number	745:754	the number of surviving neurons	745:775	NGF released from alginate (Alg) microspheres on SF scaffold (SF/Alg composites scaffolds) to the central lesion site of SCI significantly enhanced the sparing of spinal cord tissue and increased the number of surviving neurons.
28482594	3	1	theme	with/without	439:450	arg1	approach					516:523	a therapeutic approach	502:523	a therapeutic approach for spinal cord repair	502:546	In this study, we introduced a silk fibroin (SF)-based neurobridge as scaffold enriched with/without nerve growth factor (NGF) that can be utilized as a therapeutic approach for spinal cord repair.
28482594	3	1	theme	with/without	439:450	arg1	NGF					473:475	NGF	473:475	NGF	473:475	In this study, we introduced a silk fibroin (SF)-based neurobridge as scaffold enriched with/without nerve growth factor (NGF) that can be utilized as a therapeutic approach for spinal cord repair.
28482594	3	1	theme	with/without	439:450	arg1	factor					465:470	with/without nerve growth factor	439:470	with/without nerve growth factor (NGF) that can be utilized as a therapeutic approach for spinal cord repair	439:546	In this study, we introduced a silk fibroin (SF)-based neurobridge as scaffold enriched with/without nerve growth factor (NGF) that can be utilized as a therapeutic approach for spinal cord repair.
28482594	0	2	theme	injury	64:69	arg1	repair					71:76	spinal cord injury repair	52:76	spinal cord injury repair	52:76	A bridging SF/Alg composite scaffold loaded NGF for spinal cord injury repair.
28482594	4	3	theme	surviving	759:767	arg1	neurons					769:775	surviving neurons	759:775	surviving neurons	759:775	NGF released from alginate (Alg) microspheres on SF scaffold (SF/Alg composites scaffolds) to the central lesion site of SCI significantly enhanced the sparing of spinal cord tissue and increased the number of surviving neurons.
28482594	3	4	theme	nerve	452:456	arg1	approach					516:523	a therapeutic approach	502:523	a therapeutic approach for spinal cord repair	502:546	In this study, we introduced a silk fibroin (SF)-based neurobridge as scaffold enriched with/without nerve growth factor (NGF) that can be utilized as a therapeutic approach for spinal cord repair.
28482594	3	4	theme	nerve	452:456	arg1	NGF					473:475	NGF	473:475	NGF	473:475	In this study, we introduced a silk fibroin (SF)-based neurobridge as scaffold enriched with/without nerve growth factor (NGF) that can be utilized as a therapeutic approach for spinal cord repair.
28482594	3	4	theme	nerve	452:456	arg1	factor					465:470	with/without nerve growth factor	439:470	with/without nerve growth factor (NGF) that can be utilized as a therapeutic approach for spinal cord repair	439:546	In this study, we introduced a silk fibroin (SF)-based neurobridge as scaffold enriched with/without nerve growth factor (NGF) that can be utilized as a therapeutic approach for spinal cord repair.
28482594	4	5	theme	central	647:653	arg1	SCI					670:672	SCI	670:672	SCI	670:672	NGF released from alginate (Alg) microspheres on SF scaffold (SF/Alg composites scaffolds) to the central lesion site of SCI significantly enhanced the sparing of spinal cord tissue and increased the number of surviving neurons.
28482594	4	5	theme	central	647:653	arg1	site					662:665	the central lesion site	643:665	the central lesion site of SCI	643:672	NGF released from alginate (Alg) microspheres on SF scaffold (SF/Alg composites scaffolds) to the central lesion site of SCI significantly enhanced the sparing of spinal cord tissue and increased the number of surviving neurons.
28482594	4	6	attach	released	553:560	arg2	NGF					549:551	NGF	549:551	NGF released from alginate (Alg) microspheres on SF scaffold (SF/Alg composites scaffolds) to the central lesion site of SCI	549:672	NGF released from alginate (Alg) microspheres on SF scaffold (SF/Alg composites scaffolds) to the central lesion site of SCI significantly enhanced the sparing of spinal cord tissue and increased the number of surviving neurons.
28482594	4	6	attach	released	553:560	arg1	microspheres					582:593	alginate (Alg) microspheres	567:593	alginate (Alg) microspheres on SF scaffold (SF/Alg composites scaffolds)	567:638	NGF released from alginate (Alg) microspheres on SF scaffold (SF/Alg composites scaffolds) to the central lesion site of SCI significantly enhanced the sparing of spinal cord tissue and increased the number of surviving neurons.
28482594	4	7	from	microspheres	582:593	arg1	scaffold					601:608	SF scaffold	598:608	SF scaffold (SF/Alg composites scaffolds)	598:638	NGF released from alginate (Alg) microspheres on SF scaffold (SF/Alg composites scaffolds) to the central lesion site of SCI significantly enhanced the sparing of spinal cord tissue and increased the number of surviving neurons.
28482594	4	7	from	microspheres	582:593	arg1	scaffolds					629:637	SF/Alg composites scaffolds	611:637	SF/Alg composites scaffolds	611:637	NGF released from alginate (Alg) microspheres on SF scaffold (SF/Alg composites scaffolds) to the central lesion site of SCI significantly enhanced the sparing of spinal cord tissue and increased the number of surviving neurons.
28482594	5	8	theme	controlled-release	846:863	arg1	microspheres					865:876	controlled-release microspheres	846:876	controlled-release microspheres	846:876	This optimal multi-disciplinary approach of combining biomaterials, controlled-release microspheres and neurotrophic factors offers a promising treatment for the injured spinal cord.
28482594	5	9	theme	combining	822:830	arg1	biomaterials					832:843	combining biomaterials	822:843	combining biomaterials	822:843	This optimal multi-disciplinary approach of combining biomaterials, controlled-release microspheres and neurotrophic factors offers a promising treatment for the injured spinal cord.
28482594	3	10	theme	-based	399:404	arg1	neurobridge					406:416	a silk fibroin (SF)-based neurobridge	380:416	a silk fibroin (SF)-based neurobridge	380:416	In this study, we introduced a silk fibroin (SF)-based neurobridge as scaffold enriched with/without nerve growth factor (NGF) that can be utilized as a therapeutic approach for spinal cord repair.
28482594	5	11	theme	promising	912:920	arg1	treatment					922:930	a promising treatment	910:930	a promising treatment for the injured spinal cord	910:958	This optimal multi-disciplinary approach of combining biomaterials, controlled-release microspheres and neurotrophic factors offers a promising treatment for the injured spinal cord.
28482594	4	12	theme	Alg	577:579	arg1	microspheres					582:593	alginate (Alg) microspheres	567:593	alginate (Alg) microspheres on SF scaffold (SF/Alg composites scaffolds)	567:638	NGF released from alginate (Alg) microspheres on SF scaffold (SF/Alg composites scaffolds) to the central lesion site of SCI significantly enhanced the sparing of spinal cord tissue and increased the number of surviving neurons.
28482594	5	13	theme	factors	895:901	arg1	approach					810:817	This optimal multi-disciplinary approach	778:817	This optimal multi-disciplinary approach of combining biomaterials, controlled-release microspheres and neurotrophic factors	778:901	This optimal multi-disciplinary approach of combining biomaterials, controlled-release microspheres and neurotrophic factors offers a promising treatment for the injured spinal cord.
28482594	4	14	theme	SCI	670:672	arg1	SCI					670:672	SCI	670:672	SCI	670:672	NGF released from alginate (Alg) microspheres on SF scaffold (SF/Alg composites scaffolds) to the central lesion site of SCI significantly enhanced the sparing of spinal cord tissue and increased the number of surviving neurons.
28482594	4	14	theme	SCI	670:672	arg1	site					662:665	the central lesion site	643:665	the central lesion site of SCI	643:672	NGF released from alginate (Alg) microspheres on SF scaffold (SF/Alg composites scaffolds) to the central lesion site of SCI significantly enhanced the sparing of spinal cord tissue and increased the number of surviving neurons.
28482594	0	15	theme	bridging	2:9	arg1	scaffold					28:35	A bridging SF/Alg composite scaffold	0:35	A bridging SF/Alg composite scaffold	0:35	A bridging SF/Alg composite scaffold loaded NGF for spinal cord injury repair.
28482594	5	16	theme	microspheres	865:876	arg1	approach					810:817	This optimal multi-disciplinary approach	778:817	This optimal multi-disciplinary approach of combining biomaterials, controlled-release microspheres and neurotrophic factors	778:901	This optimal multi-disciplinary approach of combining biomaterials, controlled-release microspheres and neurotrophic factors offers a promising treatment for the injured spinal cord.
28482594	2	17	theme	cord	345:348	arg1	repair					320:325	the repair	316:325	the repair of injured spinal cord	316:348	A bridging biomaterial scaffold that allows the axons to grow through has been investigated for the repair of injured spinal cord.
28482594	2	18	theme	spinal	338:343	arg1	cord					345:348	injured spinal cord	330:348	injured spinal cord	330:348	A bridging biomaterial scaffold that allows the axons to grow through has been investigated for the repair of injured spinal cord.
28482594	0	19	theme	composite	18:26	arg1	scaffold					28:35	A bridging SF/Alg composite scaffold	0:35	A bridging SF/Alg composite scaffold	0:35	A bridging SF/Alg composite scaffold loaded NGF for spinal cord injury repair.
28482594	5	20	theme	injured	940:946	arg1	cord					955:958	the injured spinal cord	936:958	the injured spinal cord	936:958	This optimal multi-disciplinary approach of combining biomaterials, controlled-release microspheres and neurotrophic factors offers a promising treatment for the injured spinal cord.
28482594	4	21	theme	tissue	724:729	arg1	sparing					701:707	the sparing	697:707	the sparing of spinal cord tissue	697:729	NGF released from alginate (Alg) microspheres on SF scaffold (SF/Alg composites scaffolds) to the central lesion site of SCI significantly enhanced the sparing of spinal cord tissue and increased the number of surviving neurons.
28482594	4	22	theme	lesion	655:660	arg1	SCI					670:672	SCI	670:672	SCI	670:672	NGF released from alginate (Alg) microspheres on SF scaffold (SF/Alg composites scaffolds) to the central lesion site of SCI significantly enhanced the sparing of spinal cord tissue and increased the number of surviving neurons.
28482594	4	22	theme	lesion	655:660	arg1	site					662:665	the central lesion site	643:665	the central lesion site of SCI	643:672	NGF released from alginate (Alg) microspheres on SF scaffold (SF/Alg composites scaffolds) to the central lesion site of SCI significantly enhanced the sparing of spinal cord tissue and increased the number of surviving neurons.
28482594	4	23	theme	cord	719:722	arg1	tissue					724:729	spinal cord tissue	712:729	spinal cord tissue	712:729	NGF released from alginate (Alg) microspheres on SF scaffold (SF/Alg composites scaffolds) to the central lesion site of SCI significantly enhanced the sparing of spinal cord tissue and increased the number of surviving neurons.
28482594	0	24	theme	SF/Alg	11:16	arg1	scaffold					28:35	A bridging SF/Alg composite scaffold	0:35	A bridging SF/Alg composite scaffold	0:35	A bridging SF/Alg composite scaffold loaded NGF for spinal cord injury repair.
28482594	1	25	dep	Neurons	79:85	arg1	loss					87:90	loss	87:90	loss	87:90	Neurons loss and axons degeneration after spinal cord injury (SCI) gradually give rise to result in functional motor and sensory impairment.
28482594	1	25	dep	Neurons	79:85	arg1	degeneration					102:113	degeneration	102:113	degeneration	102:113	Neurons loss and axons degeneration after spinal cord injury (SCI) gradually give rise to result in functional motor and sensory impairment.
28482594	2	26	theme	injured	330:336	arg1	cord					345:348	injured spinal cord	330:348	injured spinal cord	330:348	A bridging biomaterial scaffold that allows the axons to grow through has been investigated for the repair of injured spinal cord.
28482594	5	27	theme	biomaterials	832:843	arg1	approach					810:817	This optimal multi-disciplinary approach	778:817	This optimal multi-disciplinary approach of combining biomaterials, controlled-release microspheres and neurotrophic factors	778:901	This optimal multi-disciplinary approach of combining biomaterials, controlled-release microspheres and neurotrophic factors offers a promising treatment for the injured spinal cord.
28482594	3	28	dep	introduced	369:378	arg1	enriched					430:437	enriched	430:437	enriched with/without nerve growth factor (NGF) that can be utilized as a therapeutic approach for spinal cord repair	430:546	In this study, we introduced a silk fibroin (SF)-based neurobridge as scaffold enriched with/without nerve growth factor (NGF) that can be utilized as a therapeutic approach for spinal cord repair.
28482594	4	29	theme	alginate	567:574	arg1	microspheres					582:593	alginate (Alg) microspheres	567:593	alginate (Alg) microspheres on SF scaffold (SF/Alg composites scaffolds)	567:638	NGF released from alginate (Alg) microspheres on SF scaffold (SF/Alg composites scaffolds) to the central lesion site of SCI significantly enhanced the sparing of spinal cord tissue and increased the number of surviving neurons.
28482594	4	30	theme	SF	598:599	arg1	scaffold					601:608	SF scaffold	598:608	SF scaffold (SF/Alg composites scaffolds)	598:638	NGF released from alginate (Alg) microspheres on SF scaffold (SF/Alg composites scaffolds) to the central lesion site of SCI significantly enhanced the sparing of spinal cord tissue and increased the number of surviving neurons.
28482594	4	30	theme	SF	598:599	arg1	scaffolds					629:637	SF/Alg composites scaffolds	611:637	SF/Alg composites scaffolds	611:637	NGF released from alginate (Alg) microspheres on SF scaffold (SF/Alg composites scaffolds) to the central lesion site of SCI significantly enhanced the sparing of spinal cord tissue and increased the number of surviving neurons.
28482594	4	31	theme	spinal	712:717	arg1	tissue					724:729	spinal cord tissue	712:729	spinal cord tissue	712:729	NGF released from alginate (Alg) microspheres on SF scaffold (SF/Alg composites scaffolds) to the central lesion site of SCI significantly enhanced the sparing of spinal cord tissue and increased the number of surviving neurons.
28482594	1	32	theme	functional	179:188	arg1	motor					190:194	functional motor	179:194	functional motor	179:194	Neurons loss and axons degeneration after spinal cord injury (SCI) gradually give rise to result in functional motor and sensory impairment.
28482594	5	33	theme	optimal	783:789	arg1	approach					810:817	This optimal multi-disciplinary approach	778:817	This optimal multi-disciplinary approach of combining biomaterials, controlled-release microspheres and neurotrophic factors	778:901	This optimal multi-disciplinary approach of combining biomaterials, controlled-release microspheres and neurotrophic factors offers a promising treatment for the injured spinal cord.
28482594	4	34	theme	composites	618:627	arg1	scaffold					601:608	SF scaffold	598:608	SF scaffold (SF/Alg composites scaffolds)	598:638	NGF released from alginate (Alg) microspheres on SF scaffold (SF/Alg composites scaffolds) to the central lesion site of SCI significantly enhanced the sparing of spinal cord tissue and increased the number of surviving neurons.
28482594	4	34	theme	composites	618:627	arg1	scaffolds					629:637	SF/Alg composites scaffolds	611:637	SF/Alg composites scaffolds	611:637	NGF released from alginate (Alg) microspheres on SF scaffold (SF/Alg composites scaffolds) to the central lesion site of SCI significantly enhanced the sparing of spinal cord tissue and increased the number of surviving neurons.
28482594	3	35	theme	therapeutic	504:514	arg1	approach					516:523	a therapeutic approach	502:523	a therapeutic approach for spinal cord repair	502:546	In this study, we introduced a silk fibroin (SF)-based neurobridge as scaffold enriched with/without nerve growth factor (NGF) that can be utilized as a therapeutic approach for spinal cord repair.
28482594	3	35	theme	therapeutic	504:514	arg1	factor					465:470	with/without nerve growth factor	439:470	with/without nerve growth factor (NGF) that can be utilized as a therapeutic approach for spinal cord repair	439:546	In this study, we introduced a silk fibroin (SF)-based neurobridge as scaffold enriched with/without nerve growth factor (NGF) that can be utilized as a therapeutic approach for spinal cord repair.
28482594	4	36	theme	SF/Alg	611:616	arg1	scaffold					601:608	SF scaffold	598:608	SF scaffold (SF/Alg composites scaffolds)	598:638	NGF released from alginate (Alg) microspheres on SF scaffold (SF/Alg composites scaffolds) to the central lesion site of SCI significantly enhanced the sparing of spinal cord tissue and increased the number of surviving neurons.
28482594	4	36	theme	SF/Alg	611:616	arg1	scaffolds					629:637	SF/Alg composites scaffolds	611:637	SF/Alg composites scaffolds	611:637	NGF released from alginate (Alg) microspheres on SF scaffold (SF/Alg composites scaffolds) to the central lesion site of SCI significantly enhanced the sparing of spinal cord tissue and increased the number of surviving neurons.
28482594	5	37	theme	spinal	948:953	arg1	cord					955:958	the injured spinal cord	936:958	the injured spinal cord	936:958	This optimal multi-disciplinary approach of combining biomaterials, controlled-release microspheres and neurotrophic factors offers a promising treatment for the injured spinal cord.
28482594	1	38	theme	cord	128:131	arg1	injury					133:138	spinal cord injury	121:138	spinal cord injury (SCI)	121:144	Neurons loss and axons degeneration after spinal cord injury (SCI) gradually give rise to result in functional motor and sensory impairment.
28482594	1	38	theme	cord	128:131	arg1	SCI					141:143	SCI	141:143	SCI	141:143	Neurons loss and axons degeneration after spinal cord injury (SCI) gradually give rise to result in functional motor and sensory impairment.
28482594	2	39	theme	biomaterial	231:241	arg1	scaffold					243:250	A bridging biomaterial scaffold	220:250	A bridging biomaterial scaffold that allows the axons to grow through has been investigated for the repair of injured spinal cord	220:348	A bridging biomaterial scaffold that allows the axons to grow through has been investigated for the repair of injured spinal cord.
28482594	5	40	theme	neurotrophic	882:893	arg1	factors					895:901	neurotrophic factors	882:901	neurotrophic factors	882:901	This optimal multi-disciplinary approach of combining biomaterials, controlled-release microspheres and neurotrophic factors offers a promising treatment for the injured spinal cord.
28482594	2	41	theme	bridging	222:229	arg1	scaffold					243:250	A bridging biomaterial scaffold	220:250	A bridging biomaterial scaffold that allows the axons to grow through has been investigated for the repair of injured spinal cord	220:348	A bridging biomaterial scaffold that allows the axons to grow through has been investigated for the repair of injured spinal cord.
28482594	1	42	theme	spinal	121:126	arg1	injury					133:138	spinal cord injury	121:138	spinal cord injury (SCI)	121:144	Neurons loss and axons degeneration after spinal cord injury (SCI) gradually give rise to result in functional motor and sensory impairment.
28482594	1	42	theme	spinal	121:126	arg1	SCI					141:143	SCI	141:143	SCI	141:143	Neurons loss and axons degeneration after spinal cord injury (SCI) gradually give rise to result in functional motor and sensory impairment.
28482594	0	43	theme	cord	59:62	arg1	injury					64:69	spinal cord injury	52:69	spinal cord injury repair	52:76	A bridging SF/Alg composite scaffold loaded NGF for spinal cord injury repair.
28482594	3	44	used	utilized	490:497	arg2	factor					465:470	with/without nerve growth factor	439:470	with/without nerve growth factor (NGF) that can be utilized as a therapeutic approach for spinal cord repair	439:546	In this study, we introduced a silk fibroin (SF)-based neurobridge as scaffold enriched with/without nerve growth factor (NGF) that can be utilized as a therapeutic approach for spinal cord repair.
28482594	3	44	used	utilized	490:497	arg2	NGF					473:475	NGF	473:475	NGF	473:475	In this study, we introduced a silk fibroin (SF)-based neurobridge as scaffold enriched with/without nerve growth factor (NGF) that can be utilized as a therapeutic approach for spinal cord repair.
28482594	3	44	used	utilized	490:497	arg2	approach					516:523	a therapeutic approach	502:523	a therapeutic approach for spinal cord repair	502:546	In this study, we introduced a silk fibroin (SF)-based neurobridge as scaffold enriched with/without nerve growth factor (NGF) that can be utilized as a therapeutic approach for spinal cord repair.
28482594	3	45	theme	spinal	529:534	arg1	cord					536:539	spinal cord	529:539	spinal cord repair	529:546	In this study, we introduced a silk fibroin (SF)-based neurobridge as scaffold enriched with/without nerve growth factor (NGF) that can be utilized as a therapeutic approach for spinal cord repair.
28482594	3	46	theme	growth	458:463	arg1	approach					516:523	a therapeutic approach	502:523	a therapeutic approach for spinal cord repair	502:546	In this study, we introduced a silk fibroin (SF)-based neurobridge as scaffold enriched with/without nerve growth factor (NGF) that can be utilized as a therapeutic approach for spinal cord repair.
28482594	3	46	theme	growth	458:463	arg1	NGF					473:475	NGF	473:475	NGF	473:475	In this study, we introduced a silk fibroin (SF)-based neurobridge as scaffold enriched with/without nerve growth factor (NGF) that can be utilized as a therapeutic approach for spinal cord repair.
28482594	3	46	theme	growth	458:463	arg1	factor					465:470	with/without nerve growth factor	439:470	with/without nerve growth factor (NGF) that can be utilized as a therapeutic approach for spinal cord repair	439:546	In this study, we introduced a silk fibroin (SF)-based neurobridge as scaffold enriched with/without nerve growth factor (NGF) that can be utilized as a therapeutic approach for spinal cord repair.
28482594	1	47	theme	sensory	200:206	arg1	impairment					208:217	sensory impairment	200:217	sensory impairment	200:217	Neurons loss and axons degeneration after spinal cord injury (SCI) gradually give rise to result in functional motor and sensory impairment.
28482594	0	48	theme	spinal	52:57	arg1	injury					64:69	spinal cord injury	52:69	spinal cord injury repair	52:76	A bridging SF/Alg composite scaffold loaded NGF for spinal cord injury repair.
28482594	3	49	theme	cord	536:539	arg1	repair					541:546	spinal cord repair	529:546	spinal cord repair	529:546	In this study, we introduced a silk fibroin (SF)-based neurobridge as scaffold enriched with/without nerve growth factor (NGF) that can be utilized as a therapeutic approach for spinal cord repair.
28482594	5	50	theme	multi-disciplinary	791:808	arg1	approach					810:817	This optimal multi-disciplinary approach	778:817	This optimal multi-disciplinary approach of combining biomaterials, controlled-release microspheres and neurotrophic factors	778:901	This optimal multi-disciplinary approach of combining biomaterials, controlled-release microspheres and neurotrophic factors offers a promising treatment for the injured spinal cord.
28482529	4	0	theme	membranes	678:686	arg1	extracts					666:673	extracts	666:673	extracts of membranes produced with linen	666:706	The lowest toxicity to human fibroblasts was observed for extracts of membranes produced with linen, and incorporation in them of epidermal growth factor was able to slightly increase cell proliferation.
28482529	1	1	theme	linen	151:155	arg1	threads					130:136	Suture threads	123:136	Suture threads	123:136	Suture threads of cotton or linen, in crossed and random orientation, were added to alginate-chitosan membranes intended to wound coatings application to improve the mechanical properties.
28482529	0	2	theme	linen	71:75	arg1	fibers					77:82	cotton or linen fibers	61:82	fibers	77:82	Composite membranes of alginate and chitosan reinforced with cotton or linen fibers incorporating epidermal growth factor.
28482529	2	3	from	break	330:334	arg1	elongation					316:325	The elongation	312:325	The elongation at break	312:334	The elongation at break increased to about 5 and 8 times for membranes with linen and cotton, respectively, both in the crossed orientation.
28482529	1	4	theme	wound	247:251	arg1	application					262:272	wound coatings application	247:272	wound coatings application	247:272	Suture threads of cotton or linen, in crossed and random orientation, were added to alginate-chitosan membranes intended to wound coatings application to improve the mechanical properties.
28482529	0	5	theme	epidermal	98:106	arg1	factor					115:120	epidermal growth factor	98:120	epidermal growth factor	98:120	Composite membranes of alginate and chitosan reinforced with cotton or linen fibers incorporating epidermal growth factor.
28482529	4	6	theme	epidermal	738:746	arg1	factor					755:760	epidermal growth factor	738:760	epidermal growth factor	738:760	The lowest toxicity to human fibroblasts was observed for extracts of membranes produced with linen, and incorporation in them of epidermal growth factor was able to slightly increase cell proliferation.
28482529	1	7	theme	coatings	253:260	arg1	application					262:272	wound coatings application	247:272	wound coatings application	247:272	Suture threads of cotton or linen, in crossed and random orientation, were added to alginate-chitosan membranes intended to wound coatings application to improve the mechanical properties.
28482529	3	8	theme	membranes	520:528	arg1	opacity					505:511	opacity	505:511	opacity	505:511	The addition of the threads increased roughness and opacity of the membranes and reduced the liquid absorption capacity and water vapor transmission rate.
28482529	3	8	theme	membranes	520:528	arg1	roughness					491:499	roughness	491:499	roughness	491:499	The addition of the threads increased roughness and opacity of the membranes and reduced the liquid absorption capacity and water vapor transmission rate.
28482529	4	9	theme	factor	755:760	arg1	incorporation					713:725	incorporation	713:725	incorporation in them of epidermal growth factor	713:760	The lowest toxicity to human fibroblasts was observed for extracts of membranes produced with linen, and incorporation in them of epidermal growth factor was able to slightly increase cell proliferation.
28482529	0	10	theme	growth	108:113	arg1	factor					115:120	epidermal growth factor	98:120	epidermal growth factor	98:120	Composite membranes of alginate and chitosan reinforced with cotton or linen fibers incorporating epidermal growth factor.
28482529	1	11	from	threads	130:136	arg1	orientation					180:190	crossed and random orientation	161:190	orientation	180:190	Suture threads of cotton or linen, in crossed and random orientation, were added to alginate-chitosan membranes intended to wound coatings application to improve the mechanical properties.
28482529	4	12	theme	growth	748:753	arg1	factor					755:760	epidermal growth factor	738:760	epidermal growth factor	738:760	The lowest toxicity to human fibroblasts was observed for extracts of membranes produced with linen, and incorporation in them of epidermal growth factor was able to slightly increase cell proliferation.
28482529	1	13	theme	crossed	161:167	arg1	orientation					180:190	crossed and random orientation	161:190	orientation	180:190	Suture threads of cotton or linen, in crossed and random orientation, were added to alginate-chitosan membranes intended to wound coatings application to improve the mechanical properties.
28482529	4	14	theme	human	631:635	arg1	fibroblasts					637:647	human fibroblasts	631:647	human fibroblasts	631:647	The lowest toxicity to human fibroblasts was observed for extracts of membranes produced with linen, and incorporation in them of epidermal growth factor was able to slightly increase cell proliferation.
28482529	0	15	theme	Composite	0:8	arg1	membranes					10:18	Composite membranes	0:18	Composite membranes of alginate and chitosan	0:43	Composite membranes of alginate and chitosan reinforced with cotton or linen fibers incorporating epidermal growth factor.
28482529	4	16	from	incorporation	713:725	arg1	them					730:733	them	730:733	them	730:733	The lowest toxicity to human fibroblasts was observed for extracts of membranes produced with linen, and incorporation in them of epidermal growth factor was able to slightly increase cell proliferation.
28482529	3	17	theme	liquid	546:551	arg1	capacity					564:571	the liquid absorption capacity	542:571	the liquid absorption capacity	542:571	The addition of the threads increased roughness and opacity of the membranes and reduced the liquid absorption capacity and water vapor transmission rate.
28482529	1	18	theme	random	173:178	arg1	orientation					180:190	crossed and random orientation	161:190	orientation	180:190	Suture threads of cotton or linen, in crossed and random orientation, were added to alginate-chitosan membranes intended to wound coatings application to improve the mechanical properties.
28482529	0	19	theme	alginate	23:30	arg1	membranes					10:18	Composite membranes	0:18	Composite membranes of alginate and chitosan	0:43	Composite membranes of alginate and chitosan reinforced with cotton or linen fibers incorporating epidermal growth factor.
28482529	4	20	theme	lowest	612:617	arg1	toxicity					619:626	The lowest toxicity	608:626	The lowest toxicity to human fibroblasts	608:647	The lowest toxicity to human fibroblasts was observed for extracts of membranes produced with linen, and incorporation in them of epidermal growth factor was able to slightly increase cell proliferation.
28482529	0	21	theme	chitosan	36:43	arg1	membranes					10:18	Composite membranes	0:18	Composite membranes of alginate and chitosan	0:43	Composite membranes of alginate and chitosan reinforced with cotton or linen fibers incorporating epidermal growth factor.
28482529	1	22	theme	mechanical	289:298	arg1	properties					300:309	the mechanical properties	285:309	the mechanical properties	285:309	Suture threads of cotton or linen, in crossed and random orientation, were added to alginate-chitosan membranes intended to wound coatings application to improve the mechanical properties.
28482529	4	23	theme	cell	792:795	arg1	proliferation					797:809	cell proliferation	792:809	cell proliferation	792:809	The lowest toxicity to human fibroblasts was observed for extracts of membranes produced with linen, and incorporation in them of epidermal growth factor was able to slightly increase cell proliferation.
28482529	2	24	dep	linen	388:392	arg1	both					420:423	both	420:423	both	420:423	The elongation at break increased to about 5 and 8 times for membranes with linen and cotton, respectively, both in the crossed orientation.
28482529	2	25	theme	crossed	432:438	arg1	orientation					440:450	the crossed orientation	428:450	the crossed orientation	428:450	The elongation at break increased to about 5 and 8 times for membranes with linen and cotton, respectively, both in the crossed orientation.
28482529	3	26	theme	threads	473:479	arg1	addition					457:464	The addition	453:464	The addition of the threads	453:479	The addition of the threads increased roughness and opacity of the membranes and reduced the liquid absorption capacity and water vapor transmission rate.
28482529	3	27	theme	absorption	553:562	arg1	capacity					564:571	the liquid absorption capacity	542:571	the liquid absorption capacity	542:571	The addition of the threads increased roughness and opacity of the membranes and reduced the liquid absorption capacity and water vapor transmission rate.
28482529	1	28	theme	Suture	123:128	arg1	threads					130:136	Suture threads	123:136	Suture threads	123:136	Suture threads of cotton or linen, in crossed and random orientation, were added to alginate-chitosan membranes intended to wound coatings application to improve the mechanical properties.
28482529	3	29	theme	water	577:581	arg1	vapor					583:587	water vapor	577:587	water vapor	577:587	The addition of the threads increased roughness and opacity of the membranes and reduced the liquid absorption capacity and water vapor transmission rate.
28482529	1	30	theme	alginate-chitosan	207:223	arg1	membranes					225:233	alginate-chitosan membranes	207:233	alginate-chitosan membranes intended to wound coatings application to improve the mechanical properties	207:309	Suture threads of cotton or linen, in crossed and random orientation, were added to alginate-chitosan membranes intended to wound coatings application to improve the mechanical properties.
28482529	1	31	theme	cotton	141:146	arg1	threads					130:136	Suture threads	123:136	Suture threads	123:136	Suture threads of cotton or linen, in crossed and random orientation, were added to alginate-chitosan membranes intended to wound coatings application to improve the mechanical properties.
28482529	3	32	dep	capacity	564:571	arg1	rate					602:605	transmission rate	589:605	transmission rate	589:605	The addition of the threads increased roughness and opacity of the membranes and reduced the liquid absorption capacity and water vapor transmission rate.
28482529	0	33	theme	cotton	61:66	arg1	fibers					77:82	cotton or linen fibers	61:82	fibers	77:82	Composite membranes of alginate and chitosan reinforced with cotton or linen fibers incorporating epidermal growth factor.
28482529	3	34	theme	transmission	589:600	arg1	rate					602:605	transmission rate	589:605	transmission rate	589:605	The addition of the threads increased roughness and opacity of the membranes and reduced the liquid absorption capacity and water vapor transmission rate.
25936285	2	0	theme	medium	524:529	arg1	capacity					531:538	medium capacity	524:538	medium capacity (ratio of medium volume to the volume of flask bottle)	524:593	Results showed that the optimal temperature, initial pH, rotation speed, medium capacity (ratio of medium volume to the volume of flask bottle) and inoculums volume for the mycelium growth were 15 °C, pH 6.0, 150 rpm, 2/5 (v/v), and 3% (v/v), respectively.
25936285	2	0	theme	medium	524:529	arg1	temperature					483:493	the optimal temperature	471:493	the optimal temperature	471:493	Results showed that the optimal temperature, initial pH, rotation speed, medium capacity (ratio of medium volume to the volume of flask bottle) and inoculums volume for the mycelium growth were 15 °C, pH 6.0, 150 rpm, 2/5 (v/v), and 3% (v/v), respectively.
25936285	1	1	theme	laser	379:383	arg1	scattering					391:400	multi-angel laser light scattering	367:400	multi-angel laser light scattering	367:400	The optimal fermentation conditions and medium for the production of bioactive polysaccharides from the mycelium of Cordyceps sinensis fungus UM01 were investigated by using orthogonal design and high performance size exclusion chromatography coupled with multi-angel laser light scattering and refractive index detector (HPSEC-MALLS-RID).
25936285	2	2	dep	capacity	531:538	arg1	ratio					541:545	ratio	541:545	ratio of medium volume to the volume of flask bottle	541:592	Results showed that the optimal temperature, initial pH, rotation speed, medium capacity (ratio of medium volume to the volume of flask bottle) and inoculums volume for the mycelium growth were 15 °C, pH 6.0, 150 rpm, 2/5 (v/v), and 3% (v/v), respectively.
25936285	3	3	theme	C.	768:769	arg1	fungus					780:785	C. sinensis fungus UM01	768:790	C. sinensis fungus UM01	768:790	Furthermore, bioactive polysaccharides from the mycelium of C. sinensis fungus UM01 were determined as polysaccharide fractions with the molecular weight above 10 kDa.
25936285	0	4	theme	sinensis	89:96	arg1	fungus					98:103	Cordyceps sinensis fungus UM01	79:108	Cordyceps sinensis fungus UM01	79:108	Fermentation optimization for the production of bioactive polysaccharides from Cordyceps sinensis fungus UM01.
25936285	1	5	theme	light	385:389	arg1	scattering					391:400	multi-angel laser light scattering	367:400	multi-angel laser light scattering	367:400	The optimal fermentation conditions and medium for the production of bioactive polysaccharides from the mycelium of Cordyceps sinensis fungus UM01 were investigated by using orthogonal design and high performance size exclusion chromatography coupled with multi-angel laser light scattering and refractive index detector (HPSEC-MALLS-RID).
25936285	1	6	from	production	166:175	arg1	mycelium					215:222	the mycelium	211:222	the mycelium of Cordyceps sinensis fungus UM01	211:256	The optimal fermentation conditions and medium for the production of bioactive polysaccharides from the mycelium of Cordyceps sinensis fungus UM01 were investigated by using orthogonal design and high performance size exclusion chromatography coupled with multi-angel laser light scattering and refractive index detector (HPSEC-MALLS-RID).
25936285	3	7	theme	sinensis	771:778	arg1	fungus					780:785	C. sinensis fungus UM01	768:790	C. sinensis fungus UM01	768:790	Furthermore, bioactive polysaccharides from the mycelium of C. sinensis fungus UM01 were determined as polysaccharide fractions with the molecular weight above 10 kDa.
25936285	2	8	theme	volume	557:562	arg1	ratio					541:545	ratio	541:545	ratio of medium volume to the volume of flask bottle	541:592	Results showed that the optimal temperature, initial pH, rotation speed, medium capacity (ratio of medium volume to the volume of flask bottle) and inoculums volume for the mycelium growth were 15 °C, pH 6.0, 150 rpm, 2/5 (v/v), and 3% (v/v), respectively.
25936285	3	9	theme	polysaccharide	811:824	arg1	fractions					826:834	polysaccharide fractions	811:834	polysaccharide fractions with the molecular weight above 10 kDa	811:873	Furthermore, bioactive polysaccharides from the mycelium of C. sinensis fungus UM01 were determined as polysaccharide fractions with the molecular weight above 10 kDa.
25936285	3	9	theme	polysaccharide	811:824	arg1	polysaccharides					731:745	bioactive polysaccharides	721:745	bioactive polysaccharides from the mycelium of C. sinensis fungus UM01	721:790	Furthermore, bioactive polysaccharides from the mycelium of C. sinensis fungus UM01 were determined as polysaccharide fractions with the molecular weight above 10 kDa.
25936285	1	10	theme	orthogonal	285:294	arg1	design					296:301	orthogonal design	285:301	orthogonal design	285:301	The optimal fermentation conditions and medium for the production of bioactive polysaccharides from the mycelium of Cordyceps sinensis fungus UM01 were investigated by using orthogonal design and high performance size exclusion chromatography coupled with multi-angel laser light scattering and refractive index detector (HPSEC-MALLS-RID).
25936285	2	11	theme	medium	550:555	arg1	volume					557:562	medium volume	550:562	medium volume to the volume of flask bottle	550:592	Results showed that the optimal temperature, initial pH, rotation speed, medium capacity (ratio of medium volume to the volume of flask bottle) and inoculums volume for the mycelium growth were 15 °C, pH 6.0, 150 rpm, 2/5 (v/v), and 3% (v/v), respectively.
25936285	5	12	theme	fermentation	1227:1238	arg1	process					1240:1246	an efficient and controllable fermentation process	1197:1246	an efficient and controllable fermentation process	1197:1246	Results are helpful to establish an efficient and controllable fermentation process for the industrial production of bioactive polysaccharides from C. sinensis UM01, and beneficial to develop a unique health and functional product in future.
25936285	2	13	theme	inoculums	599:607	arg1	volume					609:614	inoculums volume	599:614	inoculums volume	599:614	Results showed that the optimal temperature, initial pH, rotation speed, medium capacity (ratio of medium volume to the volume of flask bottle) and inoculums volume for the mycelium growth were 15 °C, pH 6.0, 150 rpm, 2/5 (v/v), and 3% (v/v), respectively.
25936285	2	13	theme	inoculums	599:607	arg1	temperature					483:493	the optimal temperature	471:493	the optimal temperature	471:493	Results showed that the optimal temperature, initial pH, rotation speed, medium capacity (ratio of medium volume to the volume of flask bottle) and inoculums volume for the mycelium growth were 15 °C, pH 6.0, 150 rpm, 2/5 (v/v), and 3% (v/v), respectively.
25936285	5	14	theme	unique	1358:1363	arg1	health					1365:1370	unique health	1358:1370	unique health	1358:1370	Results are helpful to establish an efficient and controllable fermentation process for the industrial production of bioactive polysaccharides from C. sinensis UM01, and beneficial to develop a unique health and functional product in future.
25936285	5	15	theme	industrial	1256:1265	arg1	production					1267:1276	the industrial production	1252:1276	the industrial production of bioactive polysaccharides from C. sinensis UM01	1252:1327	Results are helpful to establish an efficient and controllable fermentation process for the industrial production of bioactive polysaccharides from C. sinensis UM01, and beneficial to develop a unique health and functional product in future.
25936285	1	16	theme	refractive	406:415	arg1	index					417:421	refractive index	406:421	refractive index detector (HPSEC-MALLS-RID)	406:448	The optimal fermentation conditions and medium for the production of bioactive polysaccharides from the mycelium of Cordyceps sinensis fungus UM01 were investigated by using orthogonal design and high performance size exclusion chromatography coupled with multi-angel laser light scattering and refractive index detector (HPSEC-MALLS-RID).
25936285	1	17	theme	bioactive	180:188	arg1	polysaccharides					190:204	bioactive polysaccharides	180:204	bioactive polysaccharides from the mycelium of Cordyceps sinensis fungus UM01	180:256	The optimal fermentation conditions and medium for the production of bioactive polysaccharides from the mycelium of Cordyceps sinensis fungus UM01 were investigated by using orthogonal design and high performance size exclusion chromatography coupled with multi-angel laser light scattering and refractive index detector (HPSEC-MALLS-RID).
25936285	1	18	theme	index	417:421	arg1	HPSEC-MALLS-RID					433:447	HPSEC-MALLS-RID	433:447	HPSEC-MALLS-RID	433:447	The optimal fermentation conditions and medium for the production of bioactive polysaccharides from the mycelium of Cordyceps sinensis fungus UM01 were investigated by using orthogonal design and high performance size exclusion chromatography coupled with multi-angel laser light scattering and refractive index detector (HPSEC-MALLS-RID).
25936285	1	18	theme	index	417:421	arg1	detector					423:430	refractive index detector	406:430	refractive index detector (HPSEC-MALLS-RID)	406:448	The optimal fermentation conditions and medium for the production of bioactive polysaccharides from the mycelium of Cordyceps sinensis fungus UM01 were investigated by using orthogonal design and high performance size exclusion chromatography coupled with multi-angel laser light scattering and refractive index detector (HPSEC-MALLS-RID).
25936285	5	19	theme	functional	1376:1385	arg1	product					1387:1393	functional product	1376:1393	functional product	1376:1393	Results are helpful to establish an efficient and controllable fermentation process for the industrial production of bioactive polysaccharides from C. sinensis UM01, and beneficial to develop a unique health and functional product in future.
25936285	4	20	theme	polysaccharides	1099:1113	arg1	amount					1075:1080	the maximum amount	1063:1080	the maximum amount of the bioactive polysaccharides (486.16±19.60 mg/L) measured by HPSEC-MALLS/RID	1063:1161	The optimal fermentation medium was determined as a composition of glucose 30.0 g/L, sucrose 30.0 g/L, KH2PO4 1.0 g/L, CaCl2 0.5 g/L, yeast extract 3.0 g/L, and MgCl2 0.1g/L according to the maximum amount of the bioactive polysaccharides (486.16±19.60 mg/L) measured by HPSEC-MALLS/RID.
25936285	4	20	theme	polysaccharides	1099:1113	arg1	polysaccharides					1099:1113	the bioactive polysaccharides	1085:1113	the bioactive polysaccharides (486.16±19.60 mg/L) measured by HPSEC-MALLS/RID	1085:1161	The optimal fermentation medium was determined as a composition of glucose 30.0 g/L, sucrose 30.0 g/L, KH2PO4 1.0 g/L, CaCl2 0.5 g/L, yeast extract 3.0 g/L, and MgCl2 0.1g/L according to the maximum amount of the bioactive polysaccharides (486.16±19.60 mg/L) measured by HPSEC-MALLS/RID.
25936285	4	20	theme	polysaccharides	1099:1113	arg1	mg/L					1129:1132	486.16±19.60 mg/L	1116:1132	486.16±19.60 mg/L	1116:1132	The optimal fermentation medium was determined as a composition of glucose 30.0 g/L, sucrose 30.0 g/L, KH2PO4 1.0 g/L, CaCl2 0.5 g/L, yeast extract 3.0 g/L, and MgCl2 0.1g/L according to the maximum amount of the bioactive polysaccharides (486.16±19.60 mg/L) measured by HPSEC-MALLS/RID.
25936285	4	21	theme	MgCl2	1037:1041	arg1	medium					901:906	The optimal fermentation medium	876:906	The optimal fermentation medium	876:906	The optimal fermentation medium was determined as a composition of glucose 30.0 g/L, sucrose 30.0 g/L, KH2PO4 1.0 g/L, CaCl2 0.5 g/L, yeast extract 3.0 g/L, and MgCl2 0.1g/L according to the maximum amount of the bioactive polysaccharides (486.16±19.60 mg/L) measured by HPSEC-MALLS/RID.
25936285	4	21	theme	MgCl2	1037:1041	arg1	0.1g/L					1043:1048	MgCl2 0.1g/L	1037:1048	MgCl2 0.1g/L	1037:1048	The optimal fermentation medium was determined as a composition of glucose 30.0 g/L, sucrose 30.0 g/L, KH2PO4 1.0 g/L, CaCl2 0.5 g/L, yeast extract 3.0 g/L, and MgCl2 0.1g/L according to the maximum amount of the bioactive polysaccharides (486.16±19.60 mg/L) measured by HPSEC-MALLS/RID.
25936285	1	22	theme	polysaccharides	190:204	arg1	production					166:175	the production	162:175	the production of bioactive polysaccharides from the mycelium of Cordyceps sinensis fungus UM01	162:256	The optimal fermentation conditions and medium for the production of bioactive polysaccharides from the mycelium of Cordyceps sinensis fungus UM01 were investigated by using orthogonal design and high performance size exclusion chromatography coupled with multi-angel laser light scattering and refractive index detector (HPSEC-MALLS-RID).
25936285	2	23	theme	initial	496:502	arg1	temperature					483:493	the optimal temperature	471:493	the optimal temperature	471:493	Results showed that the optimal temperature, initial pH, rotation speed, medium capacity (ratio of medium volume to the volume of flask bottle) and inoculums volume for the mycelium growth were 15 °C, pH 6.0, 150 rpm, 2/5 (v/v), and 3% (v/v), respectively.
25936285	2	23	theme	initial	496:502	arg1	pH					504:505	initial pH	496:505	initial pH	496:505	Results showed that the optimal temperature, initial pH, rotation speed, medium capacity (ratio of medium volume to the volume of flask bottle) and inoculums volume for the mycelium growth were 15 °C, pH 6.0, 150 rpm, 2/5 (v/v), and 3% (v/v), respectively.
25936285	0	24	from	production	34:43	arg1	fungus					98:103	Cordyceps sinensis fungus UM01	79:108	Cordyceps sinensis fungus UM01	79:108	Fermentation optimization for the production of bioactive polysaccharides from Cordyceps sinensis fungus UM01.
25936285	4	25	theme	bioactive	1089:1097	arg1	polysaccharides					1099:1113	the bioactive polysaccharides	1085:1113	the bioactive polysaccharides (486.16±19.60 mg/L) measured by HPSEC-MALLS/RID	1085:1161	The optimal fermentation medium was determined as a composition of glucose 30.0 g/L, sucrose 30.0 g/L, KH2PO4 1.0 g/L, CaCl2 0.5 g/L, yeast extract 3.0 g/L, and MgCl2 0.1g/L according to the maximum amount of the bioactive polysaccharides (486.16±19.60 mg/L) measured by HPSEC-MALLS/RID.
25936285	4	25	theme	bioactive	1089:1097	arg1	mg/L					1129:1132	486.16±19.60 mg/L	1116:1132	486.16±19.60 mg/L	1116:1132	The optimal fermentation medium was determined as a composition of glucose 30.0 g/L, sucrose 30.0 g/L, KH2PO4 1.0 g/L, CaCl2 0.5 g/L, yeast extract 3.0 g/L, and MgCl2 0.1g/L according to the maximum amount of the bioactive polysaccharides (486.16±19.60 mg/L) measured by HPSEC-MALLS/RID.
25936285	3	26	theme	molecular	845:853	arg1	weight					855:860	the molecular weight	841:860	the molecular weight above 10 kDa	841:873	Furthermore, bioactive polysaccharides from the mycelium of C. sinensis fungus UM01 were determined as polysaccharide fractions with the molecular weight above 10 kDa.
25936285	5	27	from	production	1267:1276	arg1	sinensis					1315:1322	C. sinensis UM01	1312:1327	C. sinensis UM01	1312:1327	Results are helpful to establish an efficient and controllable fermentation process for the industrial production of bioactive polysaccharides from C. sinensis UM01, and beneficial to develop a unique health and functional product in future.
25936285	3	28	with	fractions	826:834	arg1	weight					855:860	the molecular weight	841:860	the molecular weight above 10 kDa	841:873	Furthermore, bioactive polysaccharides from the mycelium of C. sinensis fungus UM01 were determined as polysaccharide fractions with the molecular weight above 10 kDa.
25936285	5	29	from	product	1387:1393	arg1	future					1398:1403	future	1398:1403	future	1398:1403	Results are helpful to establish an efficient and controllable fermentation process for the industrial production of bioactive polysaccharides from C. sinensis UM01, and beneficial to develop a unique health and functional product in future.
25936285	2	30	theme	mycelium	624:631	arg1	growth					633:638	the mycelium growth	620:638	the mycelium growth	620:638	Results showed that the optimal temperature, initial pH, rotation speed, medium capacity (ratio of medium volume to the volume of flask bottle) and inoculums volume for the mycelium growth were 15 °C, pH 6.0, 150 rpm, 2/5 (v/v), and 3% (v/v), respectively.
25936285	4	31	theme	yeast	1010:1014	arg1	g/L					1028:1030	yeast extract 3.0 g/L	1010:1030	yeast extract 3.0 g/L	1010:1030	The optimal fermentation medium was determined as a composition of glucose 30.0 g/L, sucrose 30.0 g/L, KH2PO4 1.0 g/L, CaCl2 0.5 g/L, yeast extract 3.0 g/L, and MgCl2 0.1g/L according to the maximum amount of the bioactive polysaccharides (486.16±19.60 mg/L) measured by HPSEC-MALLS/RID.
25936285	4	31	theme	yeast	1010:1014	arg1	g/L					1005:1007	CaCl2 0.5 g/L	995:1007	CaCl2 0.5 g/L	995:1007	The optimal fermentation medium was determined as a composition of glucose 30.0 g/L, sucrose 30.0 g/L, KH2PO4 1.0 g/L, CaCl2 0.5 g/L, yeast extract 3.0 g/L, and MgCl2 0.1g/L according to the maximum amount of the bioactive polysaccharides (486.16±19.60 mg/L) measured by HPSEC-MALLS/RID.
25936285	1	32	theme	high	307:310	arg1	chromatography					339:352	high performance size exclusion chromatography	307:352	high performance size exclusion chromatography coupled with multi-angel laser light scattering and refractive index detector (HPSEC-MALLS-RID)	307:448	The optimal fermentation conditions and medium for the production of bioactive polysaccharides from the mycelium of Cordyceps sinensis fungus UM01 were investigated by using orthogonal design and high performance size exclusion chromatography coupled with multi-angel laser light scattering and refractive index detector (HPSEC-MALLS-RID).
25936285	3	33	from	mycelium	756:763	arg1	fractions					826:834	polysaccharide fractions	811:834	polysaccharide fractions with the molecular weight above 10 kDa	811:873	Furthermore, bioactive polysaccharides from the mycelium of C. sinensis fungus UM01 were determined as polysaccharide fractions with the molecular weight above 10 kDa.
25936285	3	33	from	mycelium	756:763	arg1	polysaccharides					731:745	bioactive polysaccharides	721:745	bioactive polysaccharides from the mycelium of C. sinensis fungus UM01	721:790	Furthermore, bioactive polysaccharides from the mycelium of C. sinensis fungus UM01 were determined as polysaccharide fractions with the molecular weight above 10 kDa.
25936285	5	34	from	sinensis	1315:1322	arg1	production					1267:1276	the industrial production	1252:1276	the industrial production of bioactive polysaccharides from C. sinensis UM01	1252:1327	Results are helpful to establish an efficient and controllable fermentation process for the industrial production of bioactive polysaccharides from C. sinensis UM01, and beneficial to develop a unique health and functional product in future.
25936285	5	34	from	sinensis	1315:1322	arg1	polysaccharides					1291:1305	bioactive polysaccharides	1281:1305	bioactive polysaccharides from C. sinensis UM01	1281:1327	Results are helpful to establish an efficient and controllable fermentation process for the industrial production of bioactive polysaccharides from C. sinensis UM01, and beneficial to develop a unique health and functional product in future.
25936285	2	35	theme	optimal	475:481	arg1	°C					648:649	15 °C	645:649	15 °C	645:649	Results showed that the optimal temperature, initial pH, rotation speed, medium capacity (ratio of medium volume to the volume of flask bottle) and inoculums volume for the mycelium growth were 15 °C, pH 6.0, 150 rpm, 2/5 (v/v), and 3% (v/v), respectively.
25936285	2	35	theme	optimal	475:481	arg1	temperature					483:493	the optimal temperature	471:493	the optimal temperature	471:493	Results showed that the optimal temperature, initial pH, rotation speed, medium capacity (ratio of medium volume to the volume of flask bottle) and inoculums volume for the mycelium growth were 15 °C, pH 6.0, 150 rpm, 2/5 (v/v), and 3% (v/v), respectively.
25936285	2	35	theme	optimal	475:481	arg1	capacity					531:538	medium capacity	524:538	medium capacity (ratio of medium volume to the volume of flask bottle)	524:593	Results showed that the optimal temperature, initial pH, rotation speed, medium capacity (ratio of medium volume to the volume of flask bottle) and inoculums volume for the mycelium growth were 15 °C, pH 6.0, 150 rpm, 2/5 (v/v), and 3% (v/v), respectively.
25936285	2	35	theme	optimal	475:481	arg1	pH					504:505	initial pH	496:505	initial pH	496:505	Results showed that the optimal temperature, initial pH, rotation speed, medium capacity (ratio of medium volume to the volume of flask bottle) and inoculums volume for the mycelium growth were 15 °C, pH 6.0, 150 rpm, 2/5 (v/v), and 3% (v/v), respectively.
25936285	2	35	theme	optimal	475:481	arg1	volume					609:614	inoculums volume	599:614	inoculums volume	599:614	Results showed that the optimal temperature, initial pH, rotation speed, medium capacity (ratio of medium volume to the volume of flask bottle) and inoculums volume for the mycelium growth were 15 °C, pH 6.0, 150 rpm, 2/5 (v/v), and 3% (v/v), respectively.
25936285	2	35	theme	optimal	475:481	arg1	speed					517:521	rotation speed	508:521	rotation speed	508:521	Results showed that the optimal temperature, initial pH, rotation speed, medium capacity (ratio of medium volume to the volume of flask bottle) and inoculums volume for the mycelium growth were 15 °C, pH 6.0, 150 rpm, 2/5 (v/v), and 3% (v/v), respectively.
25936285	1	36	theme	performance	312:322	arg1	chromatography					339:352	high performance size exclusion chromatography	307:352	high performance size exclusion chromatography coupled with multi-angel laser light scattering and refractive index detector (HPSEC-MALLS-RID)	307:448	The optimal fermentation conditions and medium for the production of bioactive polysaccharides from the mycelium of Cordyceps sinensis fungus UM01 were investigated by using orthogonal design and high performance size exclusion chromatography coupled with multi-angel laser light scattering and refractive index detector (HPSEC-MALLS-RID).
25936285	0	37	from	fungus	98:103	arg1	production					34:43	the production	30:43	the production of bioactive polysaccharides from Cordyceps sinensis fungus UM01	30:108	Fermentation optimization for the production of bioactive polysaccharides from Cordyceps sinensis fungus UM01.
25936285	0	37	from	fungus	98:103	arg1	polysaccharides					58:72	bioactive polysaccharides	48:72	bioactive polysaccharides from Cordyceps sinensis fungus UM01	48:108	Fermentation optimization for the production of bioactive polysaccharides from Cordyceps sinensis fungus UM01.
25936285	1	38	theme	optimal	115:121	arg1	conditions					136:145	The optimal fermentation conditions	111:145	The optimal fermentation conditions	111:145	The optimal fermentation conditions and medium for the production of bioactive polysaccharides from the mycelium of Cordyceps sinensis fungus UM01 were investigated by using orthogonal design and high performance size exclusion chromatography coupled with multi-angel laser light scattering and refractive index detector (HPSEC-MALLS-RID).
25936285	5	39	theme	controllable	1214:1225	arg1	process					1240:1246	an efficient and controllable fermentation process	1197:1246	an efficient and controllable fermentation process	1197:1246	Results are helpful to establish an efficient and controllable fermentation process for the industrial production of bioactive polysaccharides from C. sinensis UM01, and beneficial to develop a unique health and functional product in future.
25936285	5	40	theme	efficient	1200:1208	arg1	process					1240:1246	an efficient and controllable fermentation process	1197:1246	an efficient and controllable fermentation process	1197:1246	Results are helpful to establish an efficient and controllable fermentation process for the industrial production of bioactive polysaccharides from C. sinensis UM01, and beneficial to develop a unique health and functional product in future.
25936285	4	41	theme	glucose	943:949	arg1	g/L					956:958	glucose 30.0 g/L	943:958	glucose 30.0 g/L	943:958	The optimal fermentation medium was determined as a composition of glucose 30.0 g/L, sucrose 30.0 g/L, KH2PO4 1.0 g/L, CaCl2 0.5 g/L, yeast extract 3.0 g/L, and MgCl2 0.1g/L according to the maximum amount of the bioactive polysaccharides (486.16±19.60 mg/L) measured by HPSEC-MALLS/RID.
25936285	1	42	theme	size	324:327	arg1	chromatography					339:352	high performance size exclusion chromatography	307:352	high performance size exclusion chromatography coupled with multi-angel laser light scattering and refractive index detector (HPSEC-MALLS-RID)	307:448	The optimal fermentation conditions and medium for the production of bioactive polysaccharides from the mycelium of Cordyceps sinensis fungus UM01 were investigated by using orthogonal design and high performance size exclusion chromatography coupled with multi-angel laser light scattering and refractive index detector (HPSEC-MALLS-RID).
25936285	5	43	theme	C.	1312:1313	arg1	sinensis					1315:1322	C. sinensis UM01	1312:1327	C. sinensis UM01	1312:1327	Results are helpful to establish an efficient and controllable fermentation process for the industrial production of bioactive polysaccharides from C. sinensis UM01, and beneficial to develop a unique health and functional product in future.
25936285	4	44	theme	extract	1016:1022	arg1	g/L					1028:1030	yeast extract 3.0 g/L	1010:1030	yeast extract 3.0 g/L	1010:1030	The optimal fermentation medium was determined as a composition of glucose 30.0 g/L, sucrose 30.0 g/L, KH2PO4 1.0 g/L, CaCl2 0.5 g/L, yeast extract 3.0 g/L, and MgCl2 0.1g/L according to the maximum amount of the bioactive polysaccharides (486.16±19.60 mg/L) measured by HPSEC-MALLS/RID.
25936285	4	44	theme	extract	1016:1022	arg1	g/L					1005:1007	CaCl2 0.5 g/L	995:1007	CaCl2 0.5 g/L	995:1007	The optimal fermentation medium was determined as a composition of glucose 30.0 g/L, sucrose 30.0 g/L, KH2PO4 1.0 g/L, CaCl2 0.5 g/L, yeast extract 3.0 g/L, and MgCl2 0.1g/L according to the maximum amount of the bioactive polysaccharides (486.16±19.60 mg/L) measured by HPSEC-MALLS/RID.
25936285	1	45	theme	Cordyceps	227:235	arg1	UM01					253:256	Cordyceps sinensis fungus UM01	227:256	Cordyceps sinensis fungus UM01	227:256	The optimal fermentation conditions and medium for the production of bioactive polysaccharides from the mycelium of Cordyceps sinensis fungus UM01 were investigated by using orthogonal design and high performance size exclusion chromatography coupled with multi-angel laser light scattering and refractive index detector (HPSEC-MALLS-RID).
25936285	2	46	theme	rotation	508:515	arg1	temperature					483:493	the optimal temperature	471:493	the optimal temperature	471:493	Results showed that the optimal temperature, initial pH, rotation speed, medium capacity (ratio of medium volume to the volume of flask bottle) and inoculums volume for the mycelium growth were 15 °C, pH 6.0, 150 rpm, 2/5 (v/v), and 3% (v/v), respectively.
25936285	2	46	theme	rotation	508:515	arg1	speed					517:521	rotation speed	508:521	rotation speed	508:521	Results showed that the optimal temperature, initial pH, rotation speed, medium capacity (ratio of medium volume to the volume of flask bottle) and inoculums volume for the mycelium growth were 15 °C, pH 6.0, 150 rpm, 2/5 (v/v), and 3% (v/v), respectively.
25936285	1	47	theme	exclusion	329:337	arg1	chromatography					339:352	high performance size exclusion chromatography	307:352	high performance size exclusion chromatography coupled with multi-angel laser light scattering and refractive index detector (HPSEC-MALLS-RID)	307:448	The optimal fermentation conditions and medium for the production of bioactive polysaccharides from the mycelium of Cordyceps sinensis fungus UM01 were investigated by using orthogonal design and high performance size exclusion chromatography coupled with multi-angel laser light scattering and refractive index detector (HPSEC-MALLS-RID).
25936285	1	48	theme	sinensis	237:244	arg1	UM01					253:256	Cordyceps sinensis fungus UM01	227:256	Cordyceps sinensis fungus UM01	227:256	The optimal fermentation conditions and medium for the production of bioactive polysaccharides from the mycelium of Cordyceps sinensis fungus UM01 were investigated by using orthogonal design and high performance size exclusion chromatography coupled with multi-angel laser light scattering and refractive index detector (HPSEC-MALLS-RID).
25936285	0	49	theme	polysaccharides	58:72	arg1	production					34:43	the production	30:43	the production of bioactive polysaccharides from Cordyceps sinensis fungus UM01	30:108	Fermentation optimization for the production of bioactive polysaccharides from Cordyceps sinensis fungus UM01.
25936285	4	50	theme	fermentation	888:899	arg1	medium					901:906	The optimal fermentation medium	876:906	The optimal fermentation medium	876:906	The optimal fermentation medium was determined as a composition of glucose 30.0 g/L, sucrose 30.0 g/L, KH2PO4 1.0 g/L, CaCl2 0.5 g/L, yeast extract 3.0 g/L, and MgCl2 0.1g/L according to the maximum amount of the bioactive polysaccharides (486.16±19.60 mg/L) measured by HPSEC-MALLS/RID.
25936285	4	50	theme	fermentation	888:899	arg1	g/L					1005:1007	CaCl2 0.5 g/L	995:1007	CaCl2 0.5 g/L	995:1007	The optimal fermentation medium was determined as a composition of glucose 30.0 g/L, sucrose 30.0 g/L, KH2PO4 1.0 g/L, CaCl2 0.5 g/L, yeast extract 3.0 g/L, and MgCl2 0.1g/L according to the maximum amount of the bioactive polysaccharides (486.16±19.60 mg/L) measured by HPSEC-MALLS/RID.
25936285	4	50	theme	fermentation	888:899	arg1	0.1g/L					1043:1048	MgCl2 0.1g/L	1037:1048	MgCl2 0.1g/L	1037:1048	The optimal fermentation medium was determined as a composition of glucose 30.0 g/L, sucrose 30.0 g/L, KH2PO4 1.0 g/L, CaCl2 0.5 g/L, yeast extract 3.0 g/L, and MgCl2 0.1g/L according to the maximum amount of the bioactive polysaccharides (486.16±19.60 mg/L) measured by HPSEC-MALLS/RID.
25936285	4	50	theme	fermentation	888:899	arg1	composition					928:938	a composition	926:938	a composition of glucose 30.0 g/L	926:958	The optimal fermentation medium was determined as a composition of glucose 30.0 g/L, sucrose 30.0 g/L, KH2PO4 1.0 g/L, CaCl2 0.5 g/L, yeast extract 3.0 g/L, and MgCl2 0.1g/L according to the maximum amount of the bioactive polysaccharides (486.16±19.60 mg/L) measured by HPSEC-MALLS/RID.
25936285	4	50	theme	fermentation	888:899	arg1	g/L					974:976	sucrose 30.0 g/L	961:976	sucrose 30.0 g/L	961:976	The optimal fermentation medium was determined as a composition of glucose 30.0 g/L, sucrose 30.0 g/L, KH2PO4 1.0 g/L, CaCl2 0.5 g/L, yeast extract 3.0 g/L, and MgCl2 0.1g/L according to the maximum amount of the bioactive polysaccharides (486.16±19.60 mg/L) measured by HPSEC-MALLS/RID.
25936285	4	51	theme	maximum	1067:1073	arg1	amount					1075:1080	the maximum amount	1063:1080	the maximum amount of the bioactive polysaccharides (486.16±19.60 mg/L) measured by HPSEC-MALLS/RID	1063:1161	The optimal fermentation medium was determined as a composition of glucose 30.0 g/L, sucrose 30.0 g/L, KH2PO4 1.0 g/L, CaCl2 0.5 g/L, yeast extract 3.0 g/L, and MgCl2 0.1g/L according to the maximum amount of the bioactive polysaccharides (486.16±19.60 mg/L) measured by HPSEC-MALLS/RID.
25936285	4	51	theme	maximum	1067:1073	arg1	polysaccharides					1099:1113	the bioactive polysaccharides	1085:1113	the bioactive polysaccharides (486.16±19.60 mg/L) measured by HPSEC-MALLS/RID	1085:1161	The optimal fermentation medium was determined as a composition of glucose 30.0 g/L, sucrose 30.0 g/L, KH2PO4 1.0 g/L, CaCl2 0.5 g/L, yeast extract 3.0 g/L, and MgCl2 0.1g/L according to the maximum amount of the bioactive polysaccharides (486.16±19.60 mg/L) measured by HPSEC-MALLS/RID.
25936285	4	51	theme	maximum	1067:1073	arg1	mg/L					1129:1132	486.16±19.60 mg/L	1116:1132	486.16±19.60 mg/L	1116:1132	The optimal fermentation medium was determined as a composition of glucose 30.0 g/L, sucrose 30.0 g/L, KH2PO4 1.0 g/L, CaCl2 0.5 g/L, yeast extract 3.0 g/L, and MgCl2 0.1g/L according to the maximum amount of the bioactive polysaccharides (486.16±19.60 mg/L) measured by HPSEC-MALLS/RID.
25936285	1	52	theme	fermentation	123:134	arg1	conditions					136:145	The optimal fermentation conditions	111:145	The optimal fermentation conditions	111:145	The optimal fermentation conditions and medium for the production of bioactive polysaccharides from the mycelium of Cordyceps sinensis fungus UM01 were investigated by using orthogonal design and high performance size exclusion chromatography coupled with multi-angel laser light scattering and refractive index detector (HPSEC-MALLS-RID).
25936285	0	53	theme	bioactive	48:56	arg1	polysaccharides					58:72	bioactive polysaccharides	48:72	bioactive polysaccharides from Cordyceps sinensis fungus UM01	48:108	Fermentation optimization for the production of bioactive polysaccharides from Cordyceps sinensis fungus UM01.
25936285	1	54	from	mycelium	215:222	arg1	production					166:175	the production	162:175	the production of bioactive polysaccharides from the mycelium of Cordyceps sinensis fungus UM01	162:256	The optimal fermentation conditions and medium for the production of bioactive polysaccharides from the mycelium of Cordyceps sinensis fungus UM01 were investigated by using orthogonal design and high performance size exclusion chromatography coupled with multi-angel laser light scattering and refractive index detector (HPSEC-MALLS-RID).
25936285	1	54	from	mycelium	215:222	arg1	polysaccharides					190:204	bioactive polysaccharides	180:204	bioactive polysaccharides from the mycelium of Cordyceps sinensis fungus UM01	180:256	The optimal fermentation conditions and medium for the production of bioactive polysaccharides from the mycelium of Cordyceps sinensis fungus UM01 were investigated by using orthogonal design and high performance size exclusion chromatography coupled with multi-angel laser light scattering and refractive index detector (HPSEC-MALLS-RID).
25936285	4	55	theme	CaCl2	995:999	arg1	medium					901:906	The optimal fermentation medium	876:906	The optimal fermentation medium	876:906	The optimal fermentation medium was determined as a composition of glucose 30.0 g/L, sucrose 30.0 g/L, KH2PO4 1.0 g/L, CaCl2 0.5 g/L, yeast extract 3.0 g/L, and MgCl2 0.1g/L according to the maximum amount of the bioactive polysaccharides (486.16±19.60 mg/L) measured by HPSEC-MALLS/RID.
25936285	4	55	theme	CaCl2	995:999	arg1	g/L					1028:1030	yeast extract 3.0 g/L	1010:1030	yeast extract 3.0 g/L	1010:1030	The optimal fermentation medium was determined as a composition of glucose 30.0 g/L, sucrose 30.0 g/L, KH2PO4 1.0 g/L, CaCl2 0.5 g/L, yeast extract 3.0 g/L, and MgCl2 0.1g/L according to the maximum amount of the bioactive polysaccharides (486.16±19.60 mg/L) measured by HPSEC-MALLS/RID.
25936285	4	55	theme	CaCl2	995:999	arg1	g/L					1005:1007	CaCl2 0.5 g/L	995:1007	CaCl2 0.5 g/L	995:1007	The optimal fermentation medium was determined as a composition of glucose 30.0 g/L, sucrose 30.0 g/L, KH2PO4 1.0 g/L, CaCl2 0.5 g/L, yeast extract 3.0 g/L, and MgCl2 0.1g/L according to the maximum amount of the bioactive polysaccharides (486.16±19.60 mg/L) measured by HPSEC-MALLS/RID.
25936285	2	56	theme	bottle	587:592	arg1	flask					581:585	flask bottle	581:592	flask bottle	581:592	Results showed that the optimal temperature, initial pH, rotation speed, medium capacity (ratio of medium volume to the volume of flask bottle) and inoculums volume for the mycelium growth were 15 °C, pH 6.0, 150 rpm, 2/5 (v/v), and 3% (v/v), respectively.
25936285	5	57	theme	polysaccharides	1291:1305	arg1	production					1267:1276	the industrial production	1252:1276	the industrial production of bioactive polysaccharides from C. sinensis UM01	1252:1327	Results are helpful to establish an efficient and controllable fermentation process for the industrial production of bioactive polysaccharides from C. sinensis UM01, and beneficial to develop a unique health and functional product in future.
25936285	5	58	dep	health	1365:1370	arg1	a					1356:1356	a	1356:1356	a	1356:1356	Results are helpful to establish an efficient and controllable fermentation process for the industrial production of bioactive polysaccharides from C. sinensis UM01, and beneficial to develop a unique health and functional product in future.
25936285	1	59	theme	fungus	246:251	arg1	UM01					253:256	Cordyceps sinensis fungus UM01	227:256	Cordyceps sinensis fungus UM01	227:256	The optimal fermentation conditions and medium for the production of bioactive polysaccharides from the mycelium of Cordyceps sinensis fungus UM01 were investigated by using orthogonal design and high performance size exclusion chromatography coupled with multi-angel laser light scattering and refractive index detector (HPSEC-MALLS-RID).
25936285	2	60	theme	flask	581:585	arg1	volume					571:576	the volume	567:576	the volume of flask bottle	567:592	Results showed that the optimal temperature, initial pH, rotation speed, medium capacity (ratio of medium volume to the volume of flask bottle) and inoculums volume for the mycelium growth were 15 °C, pH 6.0, 150 rpm, 2/5 (v/v), and 3% (v/v), respectively.
25936285	5	61	from	health	1365:1370	arg1	future					1398:1403	future	1398:1403	future	1398:1403	Results are helpful to establish an efficient and controllable fermentation process for the industrial production of bioactive polysaccharides from C. sinensis UM01, and beneficial to develop a unique health and functional product in future.
25936285	3	62	theme	fungus	780:785	arg1	mycelium					756:763	the mycelium	752:763	the mycelium of C. sinensis fungus UM01	752:790	Furthermore, bioactive polysaccharides from the mycelium of C. sinensis fungus UM01 were determined as polysaccharide fractions with the molecular weight above 10 kDa.
25936285	1	63	theme	UM01	253:256	arg1	mycelium					215:222	the mycelium	211:222	the mycelium of Cordyceps sinensis fungus UM01	211:256	The optimal fermentation conditions and medium for the production of bioactive polysaccharides from the mycelium of Cordyceps sinensis fungus UM01 were investigated by using orthogonal design and high performance size exclusion chromatography coupled with multi-angel laser light scattering and refractive index detector (HPSEC-MALLS-RID).
25936285	0	64	theme	Cordyceps	79:87	arg1	fungus					98:103	Cordyceps sinensis fungus UM01	79:108	Cordyceps sinensis fungus UM01	79:108	Fermentation optimization for the production of bioactive polysaccharides from Cordyceps sinensis fungus UM01.
25936285	3	65	theme	bioactive	721:729	arg1	fractions					826:834	polysaccharide fractions	811:834	polysaccharide fractions with the molecular weight above 10 kDa	811:873	Furthermore, bioactive polysaccharides from the mycelium of C. sinensis fungus UM01 were determined as polysaccharide fractions with the molecular weight above 10 kDa.
25936285	3	65	theme	bioactive	721:729	arg1	polysaccharides					731:745	bioactive polysaccharides	721:745	bioactive polysaccharides from the mycelium of C. sinensis fungus UM01	721:790	Furthermore, bioactive polysaccharides from the mycelium of C. sinensis fungus UM01 were determined as polysaccharide fractions with the molecular weight above 10 kDa.
25936285	4	66	theme	g/L	956:958	arg1	g/L					990:992	KH2PO4 1.0 g/L	979:992	KH2PO4 1.0 g/L	979:992	The optimal fermentation medium was determined as a composition of glucose 30.0 g/L, sucrose 30.0 g/L, KH2PO4 1.0 g/L, CaCl2 0.5 g/L, yeast extract 3.0 g/L, and MgCl2 0.1g/L according to the maximum amount of the bioactive polysaccharides (486.16±19.60 mg/L) measured by HPSEC-MALLS/RID.
25936285	4	66	theme	g/L	956:958	arg1	composition					928:938	a composition	926:938	a composition of glucose 30.0 g/L	926:958	The optimal fermentation medium was determined as a composition of glucose 30.0 g/L, sucrose 30.0 g/L, KH2PO4 1.0 g/L, CaCl2 0.5 g/L, yeast extract 3.0 g/L, and MgCl2 0.1g/L according to the maximum amount of the bioactive polysaccharides (486.16±19.60 mg/L) measured by HPSEC-MALLS/RID.
25936285	4	66	theme	g/L	956:958	arg1	0.1g/L					1043:1048	MgCl2 0.1g/L	1037:1048	MgCl2 0.1g/L	1037:1048	The optimal fermentation medium was determined as a composition of glucose 30.0 g/L, sucrose 30.0 g/L, KH2PO4 1.0 g/L, CaCl2 0.5 g/L, yeast extract 3.0 g/L, and MgCl2 0.1g/L according to the maximum amount of the bioactive polysaccharides (486.16±19.60 mg/L) measured by HPSEC-MALLS/RID.
25936285	4	66	theme	g/L	956:958	arg1	g/L					974:976	sucrose 30.0 g/L	961:976	sucrose 30.0 g/L	961:976	The optimal fermentation medium was determined as a composition of glucose 30.0 g/L, sucrose 30.0 g/L, KH2PO4 1.0 g/L, CaCl2 0.5 g/L, yeast extract 3.0 g/L, and MgCl2 0.1g/L according to the maximum amount of the bioactive polysaccharides (486.16±19.60 mg/L) measured by HPSEC-MALLS/RID.
25936285	4	66	theme	g/L	956:958	arg1	medium					901:906	The optimal fermentation medium	876:906	The optimal fermentation medium	876:906	The optimal fermentation medium was determined as a composition of glucose 30.0 g/L, sucrose 30.0 g/L, KH2PO4 1.0 g/L, CaCl2 0.5 g/L, yeast extract 3.0 g/L, and MgCl2 0.1g/L according to the maximum amount of the bioactive polysaccharides (486.16±19.60 mg/L) measured by HPSEC-MALLS/RID.
25936285	4	66	theme	g/L	956:958	arg1	g/L					1028:1030	yeast extract 3.0 g/L	1010:1030	yeast extract 3.0 g/L	1010:1030	The optimal fermentation medium was determined as a composition of glucose 30.0 g/L, sucrose 30.0 g/L, KH2PO4 1.0 g/L, CaCl2 0.5 g/L, yeast extract 3.0 g/L, and MgCl2 0.1g/L according to the maximum amount of the bioactive polysaccharides (486.16±19.60 mg/L) measured by HPSEC-MALLS/RID.
25936285	4	66	theme	g/L	956:958	arg1	g/L					1005:1007	CaCl2 0.5 g/L	995:1007	CaCl2 0.5 g/L	995:1007	The optimal fermentation medium was determined as a composition of glucose 30.0 g/L, sucrose 30.0 g/L, KH2PO4 1.0 g/L, CaCl2 0.5 g/L, yeast extract 3.0 g/L, and MgCl2 0.1g/L according to the maximum amount of the bioactive polysaccharides (486.16±19.60 mg/L) measured by HPSEC-MALLS/RID.
25936285	1	67	theme	multi-angel	367:377	arg1	scattering					391:400	multi-angel laser light scattering	367:400	multi-angel laser light scattering	367:400	The optimal fermentation conditions and medium for the production of bioactive polysaccharides from the mycelium of Cordyceps sinensis fungus UM01 were investigated by using orthogonal design and high performance size exclusion chromatography coupled with multi-angel laser light scattering and refractive index detector (HPSEC-MALLS-RID).
25936285	5	68	theme	bioactive	1281:1289	arg1	polysaccharides					1291:1305	bioactive polysaccharides	1281:1305	bioactive polysaccharides from C. sinensis UM01	1281:1327	Results are helpful to establish an efficient and controllable fermentation process for the industrial production of bioactive polysaccharides from C. sinensis UM01, and beneficial to develop a unique health and functional product in future.
25936285	4	69	theme	sucrose	961:967	arg1	medium					901:906	The optimal fermentation medium	876:906	The optimal fermentation medium	876:906	The optimal fermentation medium was determined as a composition of glucose 30.0 g/L, sucrose 30.0 g/L, KH2PO4 1.0 g/L, CaCl2 0.5 g/L, yeast extract 3.0 g/L, and MgCl2 0.1g/L according to the maximum amount of the bioactive polysaccharides (486.16±19.60 mg/L) measured by HPSEC-MALLS/RID.
25936285	4	69	theme	sucrose	961:967	arg1	g/L					990:992	KH2PO4 1.0 g/L	979:992	KH2PO4 1.0 g/L	979:992	The optimal fermentation medium was determined as a composition of glucose 30.0 g/L, sucrose 30.0 g/L, KH2PO4 1.0 g/L, CaCl2 0.5 g/L, yeast extract 3.0 g/L, and MgCl2 0.1g/L according to the maximum amount of the bioactive polysaccharides (486.16±19.60 mg/L) measured by HPSEC-MALLS/RID.
25936285	4	69	theme	sucrose	961:967	arg1	g/L					974:976	sucrose 30.0 g/L	961:976	sucrose 30.0 g/L	961:976	The optimal fermentation medium was determined as a composition of glucose 30.0 g/L, sucrose 30.0 g/L, KH2PO4 1.0 g/L, CaCl2 0.5 g/L, yeast extract 3.0 g/L, and MgCl2 0.1g/L according to the maximum amount of the bioactive polysaccharides (486.16±19.60 mg/L) measured by HPSEC-MALLS/RID.
25936285	4	70	theme	KH2PO4	979:984	arg1	g/L					990:992	KH2PO4 1.0 g/L	979:992	KH2PO4 1.0 g/L	979:992	The optimal fermentation medium was determined as a composition of glucose 30.0 g/L, sucrose 30.0 g/L, KH2PO4 1.0 g/L, CaCl2 0.5 g/L, yeast extract 3.0 g/L, and MgCl2 0.1g/L according to the maximum amount of the bioactive polysaccharides (486.16±19.60 mg/L) measured by HPSEC-MALLS/RID.
25936285	4	70	theme	KH2PO4	979:984	arg1	g/L					974:976	sucrose 30.0 g/L	961:976	sucrose 30.0 g/L	961:976	The optimal fermentation medium was determined as a composition of glucose 30.0 g/L, sucrose 30.0 g/L, KH2PO4 1.0 g/L, CaCl2 0.5 g/L, yeast extract 3.0 g/L, and MgCl2 0.1g/L according to the maximum amount of the bioactive polysaccharides (486.16±19.60 mg/L) measured by HPSEC-MALLS/RID.
25936285	4	71	theme	optimal	880:886	arg1	medium					901:906	The optimal fermentation medium	876:906	The optimal fermentation medium	876:906	The optimal fermentation medium was determined as a composition of glucose 30.0 g/L, sucrose 30.0 g/L, KH2PO4 1.0 g/L, CaCl2 0.5 g/L, yeast extract 3.0 g/L, and MgCl2 0.1g/L according to the maximum amount of the bioactive polysaccharides (486.16±19.60 mg/L) measured by HPSEC-MALLS/RID.
25936285	4	71	theme	optimal	880:886	arg1	g/L					1005:1007	CaCl2 0.5 g/L	995:1007	CaCl2 0.5 g/L	995:1007	The optimal fermentation medium was determined as a composition of glucose 30.0 g/L, sucrose 30.0 g/L, KH2PO4 1.0 g/L, CaCl2 0.5 g/L, yeast extract 3.0 g/L, and MgCl2 0.1g/L according to the maximum amount of the bioactive polysaccharides (486.16±19.60 mg/L) measured by HPSEC-MALLS/RID.
25936285	4	71	theme	optimal	880:886	arg1	0.1g/L					1043:1048	MgCl2 0.1g/L	1037:1048	MgCl2 0.1g/L	1037:1048	The optimal fermentation medium was determined as a composition of glucose 30.0 g/L, sucrose 30.0 g/L, KH2PO4 1.0 g/L, CaCl2 0.5 g/L, yeast extract 3.0 g/L, and MgCl2 0.1g/L according to the maximum amount of the bioactive polysaccharides (486.16±19.60 mg/L) measured by HPSEC-MALLS/RID.
25936285	4	71	theme	optimal	880:886	arg1	composition					928:938	a composition	926:938	a composition of glucose 30.0 g/L	926:958	The optimal fermentation medium was determined as a composition of glucose 30.0 g/L, sucrose 30.0 g/L, KH2PO4 1.0 g/L, CaCl2 0.5 g/L, yeast extract 3.0 g/L, and MgCl2 0.1g/L according to the maximum amount of the bioactive polysaccharides (486.16±19.60 mg/L) measured by HPSEC-MALLS/RID.
25936285	4	71	theme	optimal	880:886	arg1	g/L					974:976	sucrose 30.0 g/L	961:976	sucrose 30.0 g/L	961:976	The optimal fermentation medium was determined as a composition of glucose 30.0 g/L, sucrose 30.0 g/L, KH2PO4 1.0 g/L, CaCl2 0.5 g/L, yeast extract 3.0 g/L, and MgCl2 0.1g/L according to the maximum amount of the bioactive polysaccharides (486.16±19.60 mg/L) measured by HPSEC-MALLS/RID.
27154759	4	0	theme	Taraxacum	1234:1242	arg1	structure					1221:1229	the reproductive structure	1204:1229	the reproductive structure of Taraxacum	1204:1242	The results point to (1) the similar distribution of AGPs in different developmental stages, (2) the absence of highly methyl-esterified homogalacturonan (HG) in transmitting tissue of ovule containing a mature embryo sac and the appearance of this pectin domain in the young seed containing the embryo and endosperm, (3) the similar pattern of low methyl-esterified pectin occurrence in both an ovule and a young seed with an embryo and endosperm in apomictic Taraxacum and (4) the presence of hemicelluloses recognised by LM25 and LM21 antibodies in the reproductive structure of Taraxacum.
27154759	0	1	dep	dandelions	135:144	arg1	Taraxacum					147:155	Taraxacum	147:155	Taraxacum	147:155	Immunodetection of some pectic, arabinogalactan proteins and hemicellulose epitopes in the micropylar transmitting tissue of apomictic dandelions (Taraxacum, Asteraceae, Lactuceae).
27154759	0	1	dep	dandelions	135:144	arg1	Lactuceae					170:178	Lactuceae	170:178	Lactuceae	170:178	Immunodetection of some pectic, arabinogalactan proteins and hemicellulose epitopes in the micropylar transmitting tissue of apomictic dandelions (Taraxacum, Asteraceae, Lactuceae).
27154759	4	2	dep	presence	1135:1142	arg1	4					1128:1128	4	1128:1128	4	1128:1128	The results point to (1) the similar distribution of AGPs in different developmental stages, (2) the absence of highly methyl-esterified homogalacturonan (HG) in transmitting tissue of ovule containing a mature embryo sac and the appearance of this pectin domain in the young seed containing the embryo and endosperm, (3) the similar pattern of low methyl-esterified pectin occurrence in both an ovule and a young seed with an embryo and endosperm in apomictic Taraxacum and (4) the presence of hemicelluloses recognised by LM25 and LM21 antibodies in the reproductive structure of Taraxacum.
27154759	1	3	theme	embryo	242:247	arg1	development					218:228	the development	214:228	the development of both the embryo and the endosperm	214:265	In apomictic Taraxacum species, the development of both the embryo and the endosperm does not require double fertilisation.
27154759	4	4	from	antibodies	1190:1199	arg1	structure					1221:1229	the reproductive structure	1204:1229	the reproductive structure of Taraxacum	1204:1242	The results point to (1) the similar distribution of AGPs in different developmental stages, (2) the absence of highly methyl-esterified homogalacturonan (HG) in transmitting tissue of ovule containing a mature embryo sac and the appearance of this pectin domain in the young seed containing the embryo and endosperm, (3) the similar pattern of low methyl-esterified pectin occurrence in both an ovule and a young seed with an embryo and endosperm in apomictic Taraxacum and (4) the presence of hemicelluloses recognised by LM25 and LM21 antibodies in the reproductive structure of Taraxacum.
27154759	4	5	dep	pattern	986:992	arg1	3					971:971	3	971:971	3	971:971	The results point to (1) the similar distribution of AGPs in different developmental stages, (2) the absence of highly methyl-esterified homogalacturonan (HG) in transmitting tissue of ovule containing a mature embryo sac and the appearance of this pectin domain in the young seed containing the embryo and endosperm, (3) the similar pattern of low methyl-esterified pectin occurrence in both an ovule and a young seed with an embryo and endosperm in apomictic Taraxacum and (4) the presence of hemicelluloses recognised by LM25 and LM21 antibodies in the reproductive structure of Taraxacum.
27154759	2	6	theme	ovular	341:346	arg1	tissue					361:366	ovular transmitting tissue	341:366	ovular transmitting tissue	341:366	However, a structural reduction of ovular transmitting tissue was not observed in apomictic dandelions.
27154759	0	7	theme	transmitting	102:113	arg1	tissue					115:120	the micropylar transmitting tissue	87:120	the micropylar transmitting tissue of apomictic dandelions (Taraxacum, Asteraceae, Lactuceae)	87:179	Immunodetection of some pectic, arabinogalactan proteins and hemicellulose epitopes in the micropylar transmitting tissue of apomictic dandelions (Taraxacum, Asteraceae, Lactuceae).
27154759	0	8	from	Immunodetection	0:14	arg1	tissue					115:120	the micropylar transmitting tissue	87:120	the micropylar transmitting tissue of apomictic dandelions (Taraxacum, Asteraceae, Lactuceae)	87:179	Immunodetection of some pectic, arabinogalactan proteins and hemicellulose epitopes in the micropylar transmitting tissue of apomictic dandelions (Taraxacum, Asteraceae, Lactuceae).
27154759	4	9	theme	ovule	837:841	arg1	tissue					827:832	tissue	827:832	tissue of ovule containing a mature embryo sac and the appearance of this pectin domain in the young seed containing the embryo and endosperm, (3) the similar pattern of low methyl-esterified pectin occurrence in both an ovule and a young seed with an embryo and endosperm in apomictic Taraxacum and (4) the presence of hemicelluloses recognised by LM25 and LM21 antibodies in the reproductive structure of Taraxacum	827:1242	The results point to (1) the similar distribution of AGPs in different developmental stages, (2) the absence of highly methyl-esterified homogalacturonan (HG) in transmitting tissue of ovule containing a mature embryo sac and the appearance of this pectin domain in the young seed containing the embryo and endosperm, (3) the similar pattern of low methyl-esterified pectin occurrence in both an ovule and a young seed with an embryo and endosperm in apomictic Taraxacum and (4) the presence of hemicelluloses recognised by LM25 and LM21 antibodies in the reproductive structure of Taraxacum.
27154759	4	10	from	stages	737:742	arg1	absence					753:759	the absence	749:759	(2) the absence of highly methyl-esterified homogalacturonan (HG)	745:809	The results point to (1) the similar distribution of AGPs in different developmental stages, (2) the absence of highly methyl-esterified homogalacturonan (HG) in transmitting tissue of ovule containing a mature embryo sac and the appearance of this pectin domain in the young seed containing the embryo and endosperm, (3) the similar pattern of low methyl-esterified pectin occurrence in both an ovule and a young seed with an embryo and endosperm in apomictic Taraxacum and (4) the presence of hemicelluloses recognised by LM25 and LM21 antibodies in the reproductive structure of Taraxacum.
27154759	4	10	from	stages	737:742	arg1	distribution					689:700	the similar distribution	677:700	(1) the similar distribution of AGPs in different developmental stages	673:742	The results point to (1) the similar distribution of AGPs in different developmental stages, (2) the absence of highly methyl-esterified homogalacturonan (HG) in transmitting tissue of ovule containing a mature embryo sac and the appearance of this pectin domain in the young seed containing the embryo and endosperm, (3) the similar pattern of low methyl-esterified pectin occurrence in both an ovule and a young seed with an embryo and endosperm in apomictic Taraxacum and (4) the presence of hemicelluloses recognised by LM25 and LM21 antibodies in the reproductive structure of Taraxacum.
27154759	3	11	theme	arabinogalactan	518:532	arg1	AGPs					544:547	AGPs	544:547	AGPs	544:547	The aim of this study was to analyse the chemical composition of the cell walls to describe the presence of arabinogalactan proteins (AGPs), hemicellulose and some pectic epitopes in the micropylar transmitting tissue of apomictic Taraxacum.
27154759	3	11	theme	arabinogalactan	518:532	arg1	hemicellulose					551:563	hemicellulose	551:563	hemicellulose	551:563	The aim of this study was to analyse the chemical composition of the cell walls to describe the presence of arabinogalactan proteins (AGPs), hemicellulose and some pectic epitopes in the micropylar transmitting tissue of apomictic Taraxacum.
27154759	3	11	theme	arabinogalactan	518:532	arg1	proteins					534:541	arabinogalactan proteins	518:541	arabinogalactan proteins (AGPs)	518:548	The aim of this study was to analyse the chemical composition of the cell walls to describe the presence of arabinogalactan proteins (AGPs), hemicellulose and some pectic epitopes in the micropylar transmitting tissue of apomictic Taraxacum.
27154759	3	11	theme	arabinogalactan	518:532	arg1	epitopes					581:588	some pectic epitopes	569:588	some pectic epitopes	569:588	The aim of this study was to analyse the chemical composition of the cell walls to describe the presence of arabinogalactan proteins (AGPs), hemicellulose and some pectic epitopes in the micropylar transmitting tissue of apomictic Taraxacum.
27154759	4	12	theme	similar	978:984	arg1	embryo					948:953	the embryo	944:953	the embryo	944:953	The results point to (1) the similar distribution of AGPs in different developmental stages, (2) the absence of highly methyl-esterified homogalacturonan (HG) in transmitting tissue of ovule containing a mature embryo sac and the appearance of this pectin domain in the young seed containing the embryo and endosperm, (3) the similar pattern of low methyl-esterified pectin occurrence in both an ovule and a young seed with an embryo and endosperm in apomictic Taraxacum and (4) the presence of hemicelluloses recognised by LM25 and LM21 antibodies in the reproductive structure of Taraxacum.
27154759	4	12	theme	similar	978:984	arg1	pattern					986:992	the similar pattern	974:992	(3) the similar pattern of low methyl-esterified pectin occurrence	970:1035	The results point to (1) the similar distribution of AGPs in different developmental stages, (2) the absence of highly methyl-esterified homogalacturonan (HG) in transmitting tissue of ovule containing a mature embryo sac and the appearance of this pectin domain in the young seed containing the embryo and endosperm, (3) the similar pattern of low methyl-esterified pectin occurrence in both an ovule and a young seed with an embryo and endosperm in apomictic Taraxacum and (4) the presence of hemicelluloses recognised by LM25 and LM21 antibodies in the reproductive structure of Taraxacum.
27154759	0	13	theme	micropylar	91:100	arg1	tissue					115:120	the micropylar transmitting tissue	87:120	the micropylar transmitting tissue of apomictic dandelions (Taraxacum, Asteraceae, Lactuceae)	87:179	Immunodetection of some pectic, arabinogalactan proteins and hemicellulose epitopes in the micropylar transmitting tissue of apomictic dandelions (Taraxacum, Asteraceae, Lactuceae).
27154759	4	14	theme	reproductive	1208:1219	arg1	structure					1221:1229	the reproductive structure	1204:1229	the reproductive structure of Taraxacum	1204:1242	The results point to (1) the similar distribution of AGPs in different developmental stages, (2) the absence of highly methyl-esterified homogalacturonan (HG) in transmitting tissue of ovule containing a mature embryo sac and the appearance of this pectin domain in the young seed containing the embryo and endosperm, (3) the similar pattern of low methyl-esterified pectin occurrence in both an ovule and a young seed with an embryo and endosperm in apomictic Taraxacum and (4) the presence of hemicelluloses recognised by LM25 and LM21 antibodies in the reproductive structure of Taraxacum.
27154759	3	15	theme	proteins	534:541	arg1	presence					506:513	the presence	502:513	the presence of arabinogalactan proteins (AGPs), hemicellulose and some pectic epitopes in the micropylar transmitting tissue of apomictic Taraxacum	502:649	The aim of this study was to analyse the chemical composition of the cell walls to describe the presence of arabinogalactan proteins (AGPs), hemicellulose and some pectic epitopes in the micropylar transmitting tissue of apomictic Taraxacum.
27154759	4	16	contain	containing	933:942	arg2	embryo					948:953	the embryo	944:953	the embryo	944:953	The results point to (1) the similar distribution of AGPs in different developmental stages, (2) the absence of highly methyl-esterified homogalacturonan (HG) in transmitting tissue of ovule containing a mature embryo sac and the appearance of this pectin domain in the young seed containing the embryo and endosperm, (3) the similar pattern of low methyl-esterified pectin occurrence in both an ovule and a young seed with an embryo and endosperm in apomictic Taraxacum and (4) the presence of hemicelluloses recognised by LM25 and LM21 antibodies in the reproductive structure of Taraxacum.
27154759	4	16	contain	containing	933:942	arg2	pattern					986:992	the similar pattern	974:992	(3) the similar pattern of low methyl-esterified pectin occurrence	970:1035	The results point to (1) the similar distribution of AGPs in different developmental stages, (2) the absence of highly methyl-esterified homogalacturonan (HG) in transmitting tissue of ovule containing a mature embryo sac and the appearance of this pectin domain in the young seed containing the embryo and endosperm, (3) the similar pattern of low methyl-esterified pectin occurrence in both an ovule and a young seed with an embryo and endosperm in apomictic Taraxacum and (4) the presence of hemicelluloses recognised by LM25 and LM21 antibodies in the reproductive structure of Taraxacum.
27154759	4	16	contain	containing	933:942	arg2	endosperm					959:967	endosperm	959:967	endosperm	959:967	The results point to (1) the similar distribution of AGPs in different developmental stages, (2) the absence of highly methyl-esterified homogalacturonan (HG) in transmitting tissue of ovule containing a mature embryo sac and the appearance of this pectin domain in the young seed containing the embryo and endosperm, (3) the similar pattern of low methyl-esterified pectin occurrence in both an ovule and a young seed with an embryo and endosperm in apomictic Taraxacum and (4) the presence of hemicelluloses recognised by LM25 and LM21 antibodies in the reproductive structure of Taraxacum.
27154759	4	16	contain	containing	933:942	arg1	seed					928:931	seed	928:931	seed	928:931	The results point to (1) the similar distribution of AGPs in different developmental stages, (2) the absence of highly methyl-esterified homogalacturonan (HG) in transmitting tissue of ovule containing a mature embryo sac and the appearance of this pectin domain in the young seed containing the embryo and endosperm, (3) the similar pattern of low methyl-esterified pectin occurrence in both an ovule and a young seed with an embryo and endosperm in apomictic Taraxacum and (4) the presence of hemicelluloses recognised by LM25 and LM21 antibodies in the reproductive structure of Taraxacum.
27154759	2	17	theme	transmitting	348:359	arg1	tissue					361:366	ovular transmitting tissue	341:366	ovular transmitting tissue	341:366	However, a structural reduction of ovular transmitting tissue was not observed in apomictic dandelions.
27154759	1	18	theme	endosperm	257:265	arg1	development					218:228	the development	214:228	the development of both the embryo and the endosperm	214:265	In apomictic Taraxacum species, the development of both the embryo and the endosperm does not require double fertilisation.
27154759	4	19	theme	domain	908:913	arg1	sac					870:872	a mature embryo sac	854:872	a mature embryo sac	854:872	The results point to (1) the similar distribution of AGPs in different developmental stages, (2) the absence of highly methyl-esterified homogalacturonan (HG) in transmitting tissue of ovule containing a mature embryo sac and the appearance of this pectin domain in the young seed containing the embryo and endosperm, (3) the similar pattern of low methyl-esterified pectin occurrence in both an ovule and a young seed with an embryo and endosperm in apomictic Taraxacum and (4) the presence of hemicelluloses recognised by LM25 and LM21 antibodies in the reproductive structure of Taraxacum.
27154759	4	19	theme	domain	908:913	arg1	appearance					882:891	the appearance	878:891	the appearance of this pectin domain in the young seed containing the embryo and endosperm, (3) the similar pattern of low methyl-esterified pectin occurrence in both an ovule and a young seed with an embryo and endosperm in apomictic Taraxacum and (4) the presence of hemicelluloses recognised by LM25 and LM21 antibodies in the reproductive structure of Taraxacum	878:1242	The results point to (1) the similar distribution of AGPs in different developmental stages, (2) the absence of highly methyl-esterified homogalacturonan (HG) in transmitting tissue of ovule containing a mature embryo sac and the appearance of this pectin domain in the young seed containing the embryo and endosperm, (3) the similar pattern of low methyl-esterified pectin occurrence in both an ovule and a young seed with an embryo and endosperm in apomictic Taraxacum and (4) the presence of hemicelluloses recognised by LM25 and LM21 antibodies in the reproductive structure of Taraxacum.
27154759	2	20	theme	apomictic	388:396	arg1	dandelions					398:407	apomictic dandelions	388:407	apomictic dandelions	388:407	However, a structural reduction of ovular transmitting tissue was not observed in apomictic dandelions.
27154759	4	21	theme	pectin	901:906	arg1	domain					908:913	this pectin domain	896:913	this pectin domain	896:913	The results point to (1) the similar distribution of AGPs in different developmental stages, (2) the absence of highly methyl-esterified homogalacturonan (HG) in transmitting tissue of ovule containing a mature embryo sac and the appearance of this pectin domain in the young seed containing the embryo and endosperm, (3) the similar pattern of low methyl-esterified pectin occurrence in both an ovule and a young seed with an embryo and endosperm in apomictic Taraxacum and (4) the presence of hemicelluloses recognised by LM25 and LM21 antibodies in the reproductive structure of Taraxacum.
27154759	4	22	contain	containing	843:852	arg2	appearance					882:891	the appearance	878:891	the appearance of this pectin domain in the young seed containing the embryo and endosperm, (3) the similar pattern of low methyl-esterified pectin occurrence in both an ovule and a young seed with an embryo and endosperm in apomictic Taraxacum and (4) the presence of hemicelluloses recognised by LM25 and LM21 antibodies in the reproductive structure of Taraxacum	878:1242	The results point to (1) the similar distribution of AGPs in different developmental stages, (2) the absence of highly methyl-esterified homogalacturonan (HG) in transmitting tissue of ovule containing a mature embryo sac and the appearance of this pectin domain in the young seed containing the embryo and endosperm, (3) the similar pattern of low methyl-esterified pectin occurrence in both an ovule and a young seed with an embryo and endosperm in apomictic Taraxacum and (4) the presence of hemicelluloses recognised by LM25 and LM21 antibodies in the reproductive structure of Taraxacum.
27154759	4	22	contain	containing	843:852	arg2	sac					870:872	a mature embryo sac	854:872	a mature embryo sac	854:872	The results point to (1) the similar distribution of AGPs in different developmental stages, (2) the absence of highly methyl-esterified homogalacturonan (HG) in transmitting tissue of ovule containing a mature embryo sac and the appearance of this pectin domain in the young seed containing the embryo and endosperm, (3) the similar pattern of low methyl-esterified pectin occurrence in both an ovule and a young seed with an embryo and endosperm in apomictic Taraxacum and (4) the presence of hemicelluloses recognised by LM25 and LM21 antibodies in the reproductive structure of Taraxacum.
27154759	4	22	contain	containing	843:852	arg1	ovule					837:841	ovule	837:841	ovule	837:841	The results point to (1) the similar distribution of AGPs in different developmental stages, (2) the absence of highly methyl-esterified homogalacturonan (HG) in transmitting tissue of ovule containing a mature embryo sac and the appearance of this pectin domain in the young seed containing the embryo and endosperm, (3) the similar pattern of low methyl-esterified pectin occurrence in both an ovule and a young seed with an embryo and endosperm in apomictic Taraxacum and (4) the presence of hemicelluloses recognised by LM25 and LM21 antibodies in the reproductive structure of Taraxacum.
27154759	4	23	theme	AGPs	705:708	arg1	absence					753:759	the absence	749:759	(2) the absence of highly methyl-esterified homogalacturonan (HG)	745:809	The results point to (1) the similar distribution of AGPs in different developmental stages, (2) the absence of highly methyl-esterified homogalacturonan (HG) in transmitting tissue of ovule containing a mature embryo sac and the appearance of this pectin domain in the young seed containing the embryo and endosperm, (3) the similar pattern of low methyl-esterified pectin occurrence in both an ovule and a young seed with an embryo and endosperm in apomictic Taraxacum and (4) the presence of hemicelluloses recognised by LM25 and LM21 antibodies in the reproductive structure of Taraxacum.
27154759	4	23	theme	AGPs	705:708	arg1	distribution					689:700	the similar distribution	677:700	(1) the similar distribution of AGPs in different developmental stages	673:742	The results point to (1) the similar distribution of AGPs in different developmental stages, (2) the absence of highly methyl-esterified homogalacturonan (HG) in transmitting tissue of ovule containing a mature embryo sac and the appearance of this pectin domain in the young seed containing the embryo and endosperm, (3) the similar pattern of low methyl-esterified pectin occurrence in both an ovule and a young seed with an embryo and endosperm in apomictic Taraxacum and (4) the presence of hemicelluloses recognised by LM25 and LM21 antibodies in the reproductive structure of Taraxacum.
27154759	4	24	from	AGPs	705:708	arg1	stages					737:742	different developmental stages	713:742	different developmental stages	713:742	The results point to (1) the similar distribution of AGPs in different developmental stages, (2) the absence of highly methyl-esterified homogalacturonan (HG) in transmitting tissue of ovule containing a mature embryo sac and the appearance of this pectin domain in the young seed containing the embryo and endosperm, (3) the similar pattern of low methyl-esterified pectin occurrence in both an ovule and a young seed with an embryo and endosperm in apomictic Taraxacum and (4) the presence of hemicelluloses recognised by LM25 and LM21 antibodies in the reproductive structure of Taraxacum.
27154759	1	25	theme	apomictic	185:193	arg1	species					205:211	apomictic Taraxacum species	185:211	apomictic Taraxacum species	185:211	In apomictic Taraxacum species, the development of both the embryo and the endosperm does not require double fertilisation.
27154759	0	26	theme	pectic	24:29	arg1	proteins					48:55	arabinogalactan proteins	32:55	arabinogalactan proteins	32:55	Immunodetection of some pectic, arabinogalactan proteins and hemicellulose epitopes in the micropylar transmitting tissue of apomictic dandelions (Taraxacum, Asteraceae, Lactuceae).
27154759	0	27	theme	dandelions	135:144	arg1	tissue					115:120	the micropylar transmitting tissue	87:120	the micropylar transmitting tissue of apomictic dandelions (Taraxacum, Asteraceae, Lactuceae)	87:179	Immunodetection of some pectic, arabinogalactan proteins and hemicellulose epitopes in the micropylar transmitting tissue of apomictic dandelions (Taraxacum, Asteraceae, Lactuceae).
27154759	4	28	theme	different	713:721	arg1	stages					737:742	different developmental stages	713:742	different developmental stages	713:742	The results point to (1) the similar distribution of AGPs in different developmental stages, (2) the absence of highly methyl-esterified homogalacturonan (HG) in transmitting tissue of ovule containing a mature embryo sac and the appearance of this pectin domain in the young seed containing the embryo and endosperm, (3) the similar pattern of low methyl-esterified pectin occurrence in both an ovule and a young seed with an embryo and endosperm in apomictic Taraxacum and (4) the presence of hemicelluloses recognised by LM25 and LM21 antibodies in the reproductive structure of Taraxacum.
27154759	4	29	theme	occurrence	1026:1035	arg1	embryo					948:953	the embryo	944:953	the embryo	944:953	The results point to (1) the similar distribution of AGPs in different developmental stages, (2) the absence of highly methyl-esterified homogalacturonan (HG) in transmitting tissue of ovule containing a mature embryo sac and the appearance of this pectin domain in the young seed containing the embryo and endosperm, (3) the similar pattern of low methyl-esterified pectin occurrence in both an ovule and a young seed with an embryo and endosperm in apomictic Taraxacum and (4) the presence of hemicelluloses recognised by LM25 and LM21 antibodies in the reproductive structure of Taraxacum.
27154759	4	29	theme	occurrence	1026:1035	arg1	pattern					986:992	the similar pattern	974:992	(3) the similar pattern of low methyl-esterified pectin occurrence	970:1035	The results point to (1) the similar distribution of AGPs in different developmental stages, (2) the absence of highly methyl-esterified homogalacturonan (HG) in transmitting tissue of ovule containing a mature embryo sac and the appearance of this pectin domain in the young seed containing the embryo and endosperm, (3) the similar pattern of low methyl-esterified pectin occurrence in both an ovule and a young seed with an embryo and endosperm in apomictic Taraxacum and (4) the presence of hemicelluloses recognised by LM25 and LM21 antibodies in the reproductive structure of Taraxacum.
27154759	1	30	theme	Taraxacum	195:203	arg1	species					205:211	apomictic Taraxacum species	185:211	apomictic Taraxacum species	185:211	In apomictic Taraxacum species, the development of both the embryo and the endosperm does not require double fertilisation.
27154759	0	31	theme	apomictic	125:133	arg1	dandelions					135:144	apomictic dandelions	125:144	apomictic dandelions (Taraxacum, Asteraceae, Lactuceae)	125:179	Immunodetection of some pectic, arabinogalactan proteins and hemicellulose epitopes in the micropylar transmitting tissue of apomictic dandelions (Taraxacum, Asteraceae, Lactuceae).
27154759	4	32	from	appearance	882:891	arg1	seed					928:931	seed	928:931	seed	928:931	The results point to (1) the similar distribution of AGPs in different developmental stages, (2) the absence of highly methyl-esterified homogalacturonan (HG) in transmitting tissue of ovule containing a mature embryo sac and the appearance of this pectin domain in the young seed containing the embryo and endosperm, (3) the similar pattern of low methyl-esterified pectin occurrence in both an ovule and a young seed with an embryo and endosperm in apomictic Taraxacum and (4) the presence of hemicelluloses recognised by LM25 and LM21 antibodies in the reproductive structure of Taraxacum.
27154759	4	33	from	endosperm	1090:1098	arg1	Taraxacum					1113:1121	apomictic Taraxacum	1103:1121	apomictic Taraxacum	1103:1121	The results point to (1) the similar distribution of AGPs in different developmental stages, (2) the absence of highly methyl-esterified homogalacturonan (HG) in transmitting tissue of ovule containing a mature embryo sac and the appearance of this pectin domain in the young seed containing the embryo and endosperm, (3) the similar pattern of low methyl-esterified pectin occurrence in both an ovule and a young seed with an embryo and endosperm in apomictic Taraxacum and (4) the presence of hemicelluloses recognised by LM25 and LM21 antibodies in the reproductive structure of Taraxacum.
27154759	4	33	from	endosperm	1090:1098	arg1	presence					1135:1142	the presence	1131:1142	(4) the presence of hemicelluloses recognised by LM25 and LM21 antibodies in the reproductive structure of Taraxacum	1127:1242	The results point to (1) the similar distribution of AGPs in different developmental stages, (2) the absence of highly methyl-esterified homogalacturonan (HG) in transmitting tissue of ovule containing a mature embryo sac and the appearance of this pectin domain in the young seed containing the embryo and endosperm, (3) the similar pattern of low methyl-esterified pectin occurrence in both an ovule and a young seed with an embryo and endosperm in apomictic Taraxacum and (4) the presence of hemicelluloses recognised by LM25 and LM21 antibodies in the reproductive structure of Taraxacum.
27154759	3	34	theme	chemical	451:458	arg1	composition					460:470	the chemical composition	447:470	the chemical composition of the cell walls to describe the presence of arabinogalactan proteins (AGPs), hemicellulose and some pectic epitopes in the micropylar transmitting tissue of apomictic Taraxacum	447:649	The aim of this study was to analyse the chemical composition of the cell walls to describe the presence of arabinogalactan proteins (AGPs), hemicellulose and some pectic epitopes in the micropylar transmitting tissue of apomictic Taraxacum.
27154759	0	35	theme	arabinogalactan	32:46	arg1	proteins					48:55	arabinogalactan proteins	32:55	arabinogalactan proteins	32:55	Immunodetection of some pectic, arabinogalactan proteins and hemicellulose epitopes in the micropylar transmitting tissue of apomictic dandelions (Taraxacum, Asteraceae, Lactuceae).
27154759	1	36	theme	double	284:289	arg1	fertilisation					291:303	double fertilisation	284:303	double fertilisation	284:303	In apomictic Taraxacum species, the development of both the embryo and the endosperm does not require double fertilisation.
27154759	3	37	theme	apomictic	631:639	arg1	Taraxacum					641:649	apomictic Taraxacum	631:649	apomictic Taraxacum	631:649	The aim of this study was to analyse the chemical composition of the cell walls to describe the presence of arabinogalactan proteins (AGPs), hemicellulose and some pectic epitopes in the micropylar transmitting tissue of apomictic Taraxacum.
27154759	3	38	attach	presence	506:513	arg2	hemicellulose					551:563	hemicellulose	551:563	hemicellulose	551:563	The aim of this study was to analyse the chemical composition of the cell walls to describe the presence of arabinogalactan proteins (AGPs), hemicellulose and some pectic epitopes in the micropylar transmitting tissue of apomictic Taraxacum.
27154759	3	38	attach	presence	506:513	arg2	AGPs					544:547	AGPs	544:547	AGPs	544:547	The aim of this study was to analyse the chemical composition of the cell walls to describe the presence of arabinogalactan proteins (AGPs), hemicellulose and some pectic epitopes in the micropylar transmitting tissue of apomictic Taraxacum.
27154759	3	38	attach	presence	506:513	arg2	proteins					534:541	arabinogalactan proteins	518:541	arabinogalactan proteins (AGPs)	518:548	The aim of this study was to analyse the chemical composition of the cell walls to describe the presence of arabinogalactan proteins (AGPs), hemicellulose and some pectic epitopes in the micropylar transmitting tissue of apomictic Taraxacum.
27154759	3	38	attach	presence	506:513	arg1	tissue					621:626	the micropylar transmitting tissue	593:626	the micropylar transmitting tissue of apomictic Taraxacum	593:649	The aim of this study was to analyse the chemical composition of the cell walls to describe the presence of arabinogalactan proteins (AGPs), hemicellulose and some pectic epitopes in the micropylar transmitting tissue of apomictic Taraxacum.
27154759	3	38	attach	presence	506:513	arg2	epitopes					581:588	some pectic epitopes	569:588	some pectic epitopes	569:588	The aim of this study was to analyse the chemical composition of the cell walls to describe the presence of arabinogalactan proteins (AGPs), hemicellulose and some pectic epitopes in the micropylar transmitting tissue of apomictic Taraxacum.
27154759	3	39	theme	study	426:430	arg1	aim					414:416	The aim	410:416	The aim of this study	410:430	The aim of this study was to analyse the chemical composition of the cell walls to describe the presence of arabinogalactan proteins (AGPs), hemicellulose and some pectic epitopes in the micropylar transmitting tissue of apomictic Taraxacum.
27154759	3	40	theme	transmitting	608:619	arg1	tissue					621:626	the micropylar transmitting tissue	593:626	the micropylar transmitting tissue of apomictic Taraxacum	593:649	The aim of this study was to analyse the chemical composition of the cell walls to describe the presence of arabinogalactan proteins (AGPs), hemicellulose and some pectic epitopes in the micropylar transmitting tissue of apomictic Taraxacum.
27154759	0	41	dep	proteins	48:55	arg1	some					19:22	some	19:22	some	19:22	Immunodetection of some pectic, arabinogalactan proteins and hemicellulose epitopes in the micropylar transmitting tissue of apomictic dandelions (Taraxacum, Asteraceae, Lactuceae).
27154759	4	42	with	seed	1066:1069	arg1	endosperm					1090:1098	endosperm	1090:1098	endosperm	1090:1098	The results point to (1) the similar distribution of AGPs in different developmental stages, (2) the absence of highly methyl-esterified homogalacturonan (HG) in transmitting tissue of ovule containing a mature embryo sac and the appearance of this pectin domain in the young seed containing the embryo and endosperm, (3) the similar pattern of low methyl-esterified pectin occurrence in both an ovule and a young seed with an embryo and endosperm in apomictic Taraxacum and (4) the presence of hemicelluloses recognised by LM25 and LM21 antibodies in the reproductive structure of Taraxacum.
27154759	4	42	with	seed	1066:1069	arg1	embryo					1079:1084	an embryo	1076:1084	an embryo	1076:1084	The results point to (1) the similar distribution of AGPs in different developmental stages, (2) the absence of highly methyl-esterified homogalacturonan (HG) in transmitting tissue of ovule containing a mature embryo sac and the appearance of this pectin domain in the young seed containing the embryo and endosperm, (3) the similar pattern of low methyl-esterified pectin occurrence in both an ovule and a young seed with an embryo and endosperm in apomictic Taraxacum and (4) the presence of hemicelluloses recognised by LM25 and LM21 antibodies in the reproductive structure of Taraxacum.
27154759	3	43	theme	walls	484:488	arg1	composition					460:470	the chemical composition	447:470	the chemical composition of the cell walls to describe the presence of arabinogalactan proteins (AGPs), hemicellulose and some pectic epitopes in the micropylar transmitting tissue of apomictic Taraxacum	447:649	The aim of this study was to analyse the chemical composition of the cell walls to describe the presence of arabinogalactan proteins (AGPs), hemicellulose and some pectic epitopes in the micropylar transmitting tissue of apomictic Taraxacum.
27154759	4	44	theme	developmental	723:735	arg1	stages					737:742	different developmental stages	713:742	different developmental stages	713:742	The results point to (1) the similar distribution of AGPs in different developmental stages, (2) the absence of highly methyl-esterified homogalacturonan (HG) in transmitting tissue of ovule containing a mature embryo sac and the appearance of this pectin domain in the young seed containing the embryo and endosperm, (3) the similar pattern of low methyl-esterified pectin occurrence in both an ovule and a young seed with an embryo and endosperm in apomictic Taraxacum and (4) the presence of hemicelluloses recognised by LM25 and LM21 antibodies in the reproductive structure of Taraxacum.
27154759	4	45	theme	homogalacturonan	789:804	arg1	absence					753:759	the absence	749:759	(2) the absence of highly methyl-esterified homogalacturonan (HG)	745:809	The results point to (1) the similar distribution of AGPs in different developmental stages, (2) the absence of highly methyl-esterified homogalacturonan (HG) in transmitting tissue of ovule containing a mature embryo sac and the appearance of this pectin domain in the young seed containing the embryo and endosperm, (3) the similar pattern of low methyl-esterified pectin occurrence in both an ovule and a young seed with an embryo and endosperm in apomictic Taraxacum and (4) the presence of hemicelluloses recognised by LM25 and LM21 antibodies in the reproductive structure of Taraxacum.
27154759	4	45	theme	homogalacturonan	789:804	arg1	distribution					689:700	the similar distribution	677:700	(1) the similar distribution of AGPs in different developmental stages	673:742	The results point to (1) the similar distribution of AGPs in different developmental stages, (2) the absence of highly methyl-esterified homogalacturonan (HG) in transmitting tissue of ovule containing a mature embryo sac and the appearance of this pectin domain in the young seed containing the embryo and endosperm, (3) the similar pattern of low methyl-esterified pectin occurrence in both an ovule and a young seed with an embryo and endosperm in apomictic Taraxacum and (4) the presence of hemicelluloses recognised by LM25 and LM21 antibodies in the reproductive structure of Taraxacum.
27154759	0	46	theme	proteins	48:55	arg1	Immunodetection					0:14	Immunodetection	0:14	Immunodetection of some pectic, arabinogalactan proteins and hemicellulose epitopes in the micropylar transmitting tissue of apomictic dandelions (Taraxacum, Asteraceae, Lactuceae).	0:180	Immunodetection of some pectic, arabinogalactan proteins and hemicellulose epitopes in the micropylar transmitting tissue of apomictic dandelions (Taraxacum, Asteraceae, Lactuceae).
27154759	4	47	theme	methyl-esterified	771:787	arg1	HG					807:808	HG	807:808	HG	807:808	The results point to (1) the similar distribution of AGPs in different developmental stages, (2) the absence of highly methyl-esterified homogalacturonan (HG) in transmitting tissue of ovule containing a mature embryo sac and the appearance of this pectin domain in the young seed containing the embryo and endosperm, (3) the similar pattern of low methyl-esterified pectin occurrence in both an ovule and a young seed with an embryo and endosperm in apomictic Taraxacum and (4) the presence of hemicelluloses recognised by LM25 and LM21 antibodies in the reproductive structure of Taraxacum.
27154759	4	47	theme	methyl-esterified	771:787	arg1	homogalacturonan					789:804	highly methyl-esterified homogalacturonan	764:804	highly methyl-esterified homogalacturonan (HG)	764:809	The results point to (1) the similar distribution of AGPs in different developmental stages, (2) the absence of highly methyl-esterified homogalacturonan (HG) in transmitting tissue of ovule containing a mature embryo sac and the appearance of this pectin domain in the young seed containing the embryo and endosperm, (3) the similar pattern of low methyl-esterified pectin occurrence in both an ovule and a young seed with an embryo and endosperm in apomictic Taraxacum and (4) the presence of hemicelluloses recognised by LM25 and LM21 antibodies in the reproductive structure of Taraxacum.
27154759	4	48	theme	apomictic	1103:1111	arg1	Taraxacum					1113:1121	apomictic Taraxacum	1103:1121	apomictic Taraxacum	1103:1121	The results point to (1) the similar distribution of AGPs in different developmental stages, (2) the absence of highly methyl-esterified homogalacturonan (HG) in transmitting tissue of ovule containing a mature embryo sac and the appearance of this pectin domain in the young seed containing the embryo and endosperm, (3) the similar pattern of low methyl-esterified pectin occurrence in both an ovule and a young seed with an embryo and endosperm in apomictic Taraxacum and (4) the presence of hemicelluloses recognised by LM25 and LM21 antibodies in the reproductive structure of Taraxacum.
27154759	3	49	theme	cell	479:482	arg1	walls					484:488	the cell walls	475:488	the cell walls	475:488	The aim of this study was to analyse the chemical composition of the cell walls to describe the presence of arabinogalactan proteins (AGPs), hemicellulose and some pectic epitopes in the micropylar transmitting tissue of apomictic Taraxacum.
27154759	4	50	theme	low	997:999	arg1	occurrence					1026:1035	low methyl-esterified pectin occurrence	997:1035	low methyl-esterified pectin occurrence	997:1035	The results point to (1) the similar distribution of AGPs in different developmental stages, (2) the absence of highly methyl-esterified homogalacturonan (HG) in transmitting tissue of ovule containing a mature embryo sac and the appearance of this pectin domain in the young seed containing the embryo and endosperm, (3) the similar pattern of low methyl-esterified pectin occurrence in both an ovule and a young seed with an embryo and endosperm in apomictic Taraxacum and (4) the presence of hemicelluloses recognised by LM25 and LM21 antibodies in the reproductive structure of Taraxacum.
27154759	4	51	theme	embryo	863:868	arg1	sac					870:872	a mature embryo sac	854:872	a mature embryo sac	854:872	The results point to (1) the similar distribution of AGPs in different developmental stages, (2) the absence of highly methyl-esterified homogalacturonan (HG) in transmitting tissue of ovule containing a mature embryo sac and the appearance of this pectin domain in the young seed containing the embryo and endosperm, (3) the similar pattern of low methyl-esterified pectin occurrence in both an ovule and a young seed with an embryo and endosperm in apomictic Taraxacum and (4) the presence of hemicelluloses recognised by LM25 and LM21 antibodies in the reproductive structure of Taraxacum.
27154759	4	52	theme	hemicelluloses	1147:1160	arg1	Taraxacum					1113:1121	apomictic Taraxacum	1103:1121	apomictic Taraxacum	1103:1121	The results point to (1) the similar distribution of AGPs in different developmental stages, (2) the absence of highly methyl-esterified homogalacturonan (HG) in transmitting tissue of ovule containing a mature embryo sac and the appearance of this pectin domain in the young seed containing the embryo and endosperm, (3) the similar pattern of low methyl-esterified pectin occurrence in both an ovule and a young seed with an embryo and endosperm in apomictic Taraxacum and (4) the presence of hemicelluloses recognised by LM25 and LM21 antibodies in the reproductive structure of Taraxacum.
27154759	4	52	theme	hemicelluloses	1147:1160	arg1	presence					1135:1142	the presence	1131:1142	(4) the presence of hemicelluloses recognised by LM25 and LM21 antibodies in the reproductive structure of Taraxacum	1127:1242	The results point to (1) the similar distribution of AGPs in different developmental stages, (2) the absence of highly methyl-esterified homogalacturonan (HG) in transmitting tissue of ovule containing a mature embryo sac and the appearance of this pectin domain in the young seed containing the embryo and endosperm, (3) the similar pattern of low methyl-esterified pectin occurrence in both an ovule and a young seed with an embryo and endosperm in apomictic Taraxacum and (4) the presence of hemicelluloses recognised by LM25 and LM21 antibodies in the reproductive structure of Taraxacum.
27154759	4	53	from	embryo	1079:1084	arg1	Taraxacum					1113:1121	apomictic Taraxacum	1103:1121	apomictic Taraxacum	1103:1121	The results point to (1) the similar distribution of AGPs in different developmental stages, (2) the absence of highly methyl-esterified homogalacturonan (HG) in transmitting tissue of ovule containing a mature embryo sac and the appearance of this pectin domain in the young seed containing the embryo and endosperm, (3) the similar pattern of low methyl-esterified pectin occurrence in both an ovule and a young seed with an embryo and endosperm in apomictic Taraxacum and (4) the presence of hemicelluloses recognised by LM25 and LM21 antibodies in the reproductive structure of Taraxacum.
27154759	4	53	from	embryo	1079:1084	arg1	presence					1135:1142	the presence	1131:1142	(4) the presence of hemicelluloses recognised by LM25 and LM21 antibodies in the reproductive structure of Taraxacum	1127:1242	The results point to (1) the similar distribution of AGPs in different developmental stages, (2) the absence of highly methyl-esterified homogalacturonan (HG) in transmitting tissue of ovule containing a mature embryo sac and the appearance of this pectin domain in the young seed containing the embryo and endosperm, (3) the similar pattern of low methyl-esterified pectin occurrence in both an ovule and a young seed with an embryo and endosperm in apomictic Taraxacum and (4) the presence of hemicelluloses recognised by LM25 and LM21 antibodies in the reproductive structure of Taraxacum.
27154759	3	54	theme	pectic	574:579	arg1	proteins					534:541	arabinogalactan proteins	518:541	arabinogalactan proteins (AGPs)	518:548	The aim of this study was to analyse the chemical composition of the cell walls to describe the presence of arabinogalactan proteins (AGPs), hemicellulose and some pectic epitopes in the micropylar transmitting tissue of apomictic Taraxacum.
27154759	3	54	theme	pectic	574:579	arg1	epitopes					581:588	some pectic epitopes	569:588	some pectic epitopes	569:588	The aim of this study was to analyse the chemical composition of the cell walls to describe the presence of arabinogalactan proteins (AGPs), hemicellulose and some pectic epitopes in the micropylar transmitting tissue of apomictic Taraxacum.
27154759	2	55	theme	structural	317:326	arg1	reduction					328:336	a structural reduction	315:336	a structural reduction of ovular transmitting tissue	315:366	However, a structural reduction of ovular transmitting tissue was not observed in apomictic dandelions.
27154759	4	56	dep	distribution	689:700	arg1	1					674:674	1	674:674	1	674:674	The results point to (1) the similar distribution of AGPs in different developmental stages, (2) the absence of highly methyl-esterified homogalacturonan (HG) in transmitting tissue of ovule containing a mature embryo sac and the appearance of this pectin domain in the young seed containing the embryo and endosperm, (3) the similar pattern of low methyl-esterified pectin occurrence in both an ovule and a young seed with an embryo and endosperm in apomictic Taraxacum and (4) the presence of hemicelluloses recognised by LM25 and LM21 antibodies in the reproductive structure of Taraxacum.
27154759	4	57	theme	mature	856:861	arg1	sac					870:872	a mature embryo sac	854:872	a mature embryo sac	854:872	The results point to (1) the similar distribution of AGPs in different developmental stages, (2) the absence of highly methyl-esterified homogalacturonan (HG) in transmitting tissue of ovule containing a mature embryo sac and the appearance of this pectin domain in the young seed containing the embryo and endosperm, (3) the similar pattern of low methyl-esterified pectin occurrence in both an ovule and a young seed with an embryo and endosperm in apomictic Taraxacum and (4) the presence of hemicelluloses recognised by LM25 and LM21 antibodies in the reproductive structure of Taraxacum.
27154759	3	58	theme	Taraxacum	641:649	arg1	tissue					621:626	the micropylar transmitting tissue	593:626	the micropylar transmitting tissue of apomictic Taraxacum	593:649	The aim of this study was to analyse the chemical composition of the cell walls to describe the presence of arabinogalactan proteins (AGPs), hemicellulose and some pectic epitopes in the micropylar transmitting tissue of apomictic Taraxacum.
27154759	3	59	theme	micropylar	597:606	arg1	tissue					621:626	the micropylar transmitting tissue	593:626	the micropylar transmitting tissue of apomictic Taraxacum	593:649	The aim of this study was to analyse the chemical composition of the cell walls to describe the presence of arabinogalactan proteins (AGPs), hemicellulose and some pectic epitopes in the micropylar transmitting tissue of apomictic Taraxacum.
27154759	4	60	theme	similar	681:687	arg1	absence					753:759	the absence	749:759	(2) the absence of highly methyl-esterified homogalacturonan (HG)	745:809	The results point to (1) the similar distribution of AGPs in different developmental stages, (2) the absence of highly methyl-esterified homogalacturonan (HG) in transmitting tissue of ovule containing a mature embryo sac and the appearance of this pectin domain in the young seed containing the embryo and endosperm, (3) the similar pattern of low methyl-esterified pectin occurrence in both an ovule and a young seed with an embryo and endosperm in apomictic Taraxacum and (4) the presence of hemicelluloses recognised by LM25 and LM21 antibodies in the reproductive structure of Taraxacum.
27154759	4	60	theme	similar	681:687	arg1	distribution					689:700	the similar distribution	677:700	(1) the similar distribution of AGPs in different developmental stages	673:742	The results point to (1) the similar distribution of AGPs in different developmental stages, (2) the absence of highly methyl-esterified homogalacturonan (HG) in transmitting tissue of ovule containing a mature embryo sac and the appearance of this pectin domain in the young seed containing the embryo and endosperm, (3) the similar pattern of low methyl-esterified pectin occurrence in both an ovule and a young seed with an embryo and endosperm in apomictic Taraxacum and (4) the presence of hemicelluloses recognised by LM25 and LM21 antibodies in the reproductive structure of Taraxacum.
27154759	4	61	theme	pectin	1019:1024	arg1	occurrence					1026:1035	low methyl-esterified pectin occurrence	997:1035	low methyl-esterified pectin occurrence	997:1035	The results point to (1) the similar distribution of AGPs in different developmental stages, (2) the absence of highly methyl-esterified homogalacturonan (HG) in transmitting tissue of ovule containing a mature embryo sac and the appearance of this pectin domain in the young seed containing the embryo and endosperm, (3) the similar pattern of low methyl-esterified pectin occurrence in both an ovule and a young seed with an embryo and endosperm in apomictic Taraxacum and (4) the presence of hemicelluloses recognised by LM25 and LM21 antibodies in the reproductive structure of Taraxacum.
27154759	4	62	from	distribution	689:700	arg1	stages					737:742	different developmental stages	713:742	different developmental stages	713:742	The results point to (1) the similar distribution of AGPs in different developmental stages, (2) the absence of highly methyl-esterified homogalacturonan (HG) in transmitting tissue of ovule containing a mature embryo sac and the appearance of this pectin domain in the young seed containing the embryo and endosperm, (3) the similar pattern of low methyl-esterified pectin occurrence in both an ovule and a young seed with an embryo and endosperm in apomictic Taraxacum and (4) the presence of hemicelluloses recognised by LM25 and LM21 antibodies in the reproductive structure of Taraxacum.
27154759	0	63	theme	epitopes	75:82	arg1	Immunodetection					0:14	Immunodetection	0:14	Immunodetection of some pectic, arabinogalactan proteins and hemicellulose epitopes in the micropylar transmitting tissue of apomictic dandelions (Taraxacum, Asteraceae, Lactuceae).	0:180	Immunodetection of some pectic, arabinogalactan proteins and hemicellulose epitopes in the micropylar transmitting tissue of apomictic dandelions (Taraxacum, Asteraceae, Lactuceae).
27154759	2	64	located	observed	376:383	arg1	dandelions					398:407	apomictic dandelions	388:407	apomictic dandelions	388:407	However, a structural reduction of ovular transmitting tissue was not observed in apomictic dandelions.
27154759	2	64	located	observed	376:383	arg2	reduction					328:336	a structural reduction	315:336	a structural reduction of ovular transmitting tissue	315:366	However, a structural reduction of ovular transmitting tissue was not observed in apomictic dandelions.
27154759	4	65	dep	absence	753:759	arg1	2					746:746	2	746:746	2	746:746	The results point to (1) the similar distribution of AGPs in different developmental stages, (2) the absence of highly methyl-esterified homogalacturonan (HG) in transmitting tissue of ovule containing a mature embryo sac and the appearance of this pectin domain in the young seed containing the embryo and endosperm, (3) the similar pattern of low methyl-esterified pectin occurrence in both an ovule and a young seed with an embryo and endosperm in apomictic Taraxacum and (4) the presence of hemicelluloses recognised by LM25 and LM21 antibodies in the reproductive structure of Taraxacum.
27154759	4	66	from	sac	870:872	arg1	seed					928:931	seed	928:931	seed	928:931	The results point to (1) the similar distribution of AGPs in different developmental stages, (2) the absence of highly methyl-esterified homogalacturonan (HG) in transmitting tissue of ovule containing a mature embryo sac and the appearance of this pectin domain in the young seed containing the embryo and endosperm, (3) the similar pattern of low methyl-esterified pectin occurrence in both an ovule and a young seed with an embryo and endosperm in apomictic Taraxacum and (4) the presence of hemicelluloses recognised by LM25 and LM21 antibodies in the reproductive structure of Taraxacum.
27154759	4	67	theme	methyl-esterified	1001:1017	arg1	occurrence					1026:1035	low methyl-esterified pectin occurrence	997:1035	low methyl-esterified pectin occurrence	997:1035	The results point to (1) the similar distribution of AGPs in different developmental stages, (2) the absence of highly methyl-esterified homogalacturonan (HG) in transmitting tissue of ovule containing a mature embryo sac and the appearance of this pectin domain in the young seed containing the embryo and endosperm, (3) the similar pattern of low methyl-esterified pectin occurrence in both an ovule and a young seed with an embryo and endosperm in apomictic Taraxacum and (4) the presence of hemicelluloses recognised by LM25 and LM21 antibodies in the reproductive structure of Taraxacum.
27154759	0	68	theme	hemicellulose	61:73	arg1	epitopes					75:82	hemicellulose epitopes	61:82	hemicellulose epitopes	61:82	Immunodetection of some pectic, arabinogalactan proteins and hemicellulose epitopes in the micropylar transmitting tissue of apomictic dandelions (Taraxacum, Asteraceae, Lactuceae).
27154759	2	69	theme	tissue	361:366	arg1	reduction					328:336	a structural reduction	315:336	a structural reduction of ovular transmitting tissue	315:366	However, a structural reduction of ovular transmitting tissue was not observed in apomictic dandelions.
27154759	3	70	from	presence	506:513	arg1	tissue					621:626	the micropylar transmitting tissue	593:626	the micropylar transmitting tissue of apomictic Taraxacum	593:649	The aim of this study was to analyse the chemical composition of the cell walls to describe the presence of arabinogalactan proteins (AGPs), hemicellulose and some pectic epitopes in the micropylar transmitting tissue of apomictic Taraxacum.
26704999	4	0	theme	sugar	459:463	arg1	composition					465:475	sugar composition	459:475	sugar composition of rhamnose (2%), arabinose (3%), mannose (3%), galactose (94%) and uronic acid (17%)	459:561	Data indicated that PGP possessed sugar composition of rhamnose (2%), arabinose (3%), mannose (3%), galactose (94%) and uronic acid (17%) confirming that PGP thus isolated is a galactan.
26704999	8	1	theme	Mucosal	1050:1056	arg1	recovery					1058:1065	Mucosal recovery	1050:1065	Mucosal recovery	1050:1065	Mucosal recovery could be attributed to cytoprotective and DNA protective ability of PGP that can help in mucosal layer regeneration.
26704999	6	2	theme	antiulcer	774:782	arg1	potency					784:790	The antiulcer potency	770:790	The antiulcer potency of PGP	770:797	The antiulcer potency of PGP was evaluated in ethanol stress induced gastric ulcer model in vivo.
26704999	3	3	theme	galactans	288:296	arg1	confirmation					302:313	confirmation	302:313	confirmation of the same by sugar analysis	302:343	The present study describes the isolation of galactans and confirmation of the same by sugar analysis and determination of anti-ulcer effect of Potato Galactan Polysaccharide (PGP).
26704999	3	3	theme	galactans	288:296	arg1	determination					349:361	determination	349:361	determination of anti-ulcer effect of Potato Galactan Polysaccharide (PGP)	349:422	The present study describes the isolation of galactans and confirmation of the same by sugar analysis and determination of anti-ulcer effect of Potato Galactan Polysaccharide (PGP).
26704999	3	3	theme	galactans	288:296	arg1	isolation					275:283	the isolation	271:283	the isolation of galactans	271:296	The present study describes the isolation of galactans and confirmation of the same by sugar analysis and determination of anti-ulcer effect of Potato Galactan Polysaccharide (PGP).
26704999	4	4	theme	rhamnose	480:487	arg1	composition					465:475	sugar composition	459:475	sugar composition of rhamnose (2%), arabinose (3%), mannose (3%), galactose (94%) and uronic acid (17%)	459:561	Data indicated that PGP possessed sugar composition of rhamnose (2%), arabinose (3%), mannose (3%), galactose (94%) and uronic acid (17%) confirming that PGP thus isolated is a galactan.
26704999	3	5	theme	same	322:325	arg1	confirmation					302:313	confirmation	302:313	confirmation of the same by sugar analysis	302:343	The present study describes the isolation of galactans and confirmation of the same by sugar analysis and determination of anti-ulcer effect of Potato Galactan Polysaccharide (PGP).
26704999	3	5	theme	same	322:325	arg1	determination					349:361	determination	349:361	determination of anti-ulcer effect of Potato Galactan Polysaccharide (PGP)	349:422	The present study describes the isolation of galactans and confirmation of the same by sugar analysis and determination of anti-ulcer effect of Potato Galactan Polysaccharide (PGP).
26704999	3	5	theme	same	322:325	arg1	isolation					275:283	the isolation	271:283	the isolation of galactans	271:296	The present study describes the isolation of galactans and confirmation of the same by sugar analysis and determination of anti-ulcer effect of Potato Galactan Polysaccharide (PGP).
26704999	9	6	theme	growth	1257:1262	arg1	assay					1275:1279	growth inhibition assay	1257:1279	growth inhibition assay followed by scanning electron microscopic studies suggesting that PGP is effective in curbing the growth of H. pylori, which is responsible for ∼70% of gastric ulcer/cancer incidences	1257:1463	Further, PGP was also effective in inhibiting Helicobacter pylori as per growth inhibition assay followed by scanning electron microscopic studies suggesting that PGP is effective in curbing the growth of H. pylori, which is responsible for ∼70% of gastric ulcer/cancer incidences.
26704999	5	7	theme	antiulcer	733:741	arg1	drug					743:746	a known antiulcer drug	725:746	a known antiulcer drug with IC50 19.3 μg/mL	725:767	PGP exhibited potent H(+), K(+)-ATPase inhibitory activity (IC50 420 μg/mL) in vitro as opposed to lansoprazole, a known antiulcer drug with IC50 19.3 μg/mL.
26704999	5	7	theme	antiulcer	733:741	arg1	lansoprazole					711:722	lansoprazole	711:722	lansoprazole	711:722	PGP exhibited potent H(+), K(+)-ATPase inhibitory activity (IC50 420 μg/mL) in vitro as opposed to lansoprazole, a known antiulcer drug with IC50 19.3 μg/mL.
26704999	4	8	theme	mannose	511:517	arg1	composition					465:475	sugar composition	459:475	sugar composition of rhamnose (2%), arabinose (3%), mannose (3%), galactose (94%) and uronic acid (17%)	459:561	Data indicated that PGP possessed sugar composition of rhamnose (2%), arabinose (3%), mannose (3%), galactose (94%) and uronic acid (17%) confirming that PGP thus isolated is a galactan.
26704999	9	9	theme	microscopic	1311:1321	arg1	studies					1323:1329	scanning electron microscopic studies	1293:1329	scanning electron microscopic studies suggesting that PGP is effective in curbing the growth of H. pylori, which is responsible for ∼70% of gastric ulcer/cancer incidences	1293:1463	Further, PGP was also effective in inhibiting Helicobacter pylori as per growth inhibition assay followed by scanning electron microscopic studies suggesting that PGP is effective in curbing the growth of H. pylori, which is responsible for ∼70% of gastric ulcer/cancer incidences.
26704999	9	10	theme	H.	1389:1390	arg1	growth					1379:1384	the growth	1375:1384	the growth of H. pylori, which is responsible for ∼70% of gastric ulcer/cancer incidences	1375:1463	Further, PGP was also effective in inhibiting Helicobacter pylori as per growth inhibition assay followed by scanning electron microscopic studies suggesting that PGP is effective in curbing the growth of H. pylori, which is responsible for ∼70% of gastric ulcer/cancer incidences.
26704999	8	11	theme	DNA	1109:1111	arg1	ability					1124:1130	cytoprotective and DNA protective ability	1090:1130	ability	1124:1130	Mucosal recovery could be attributed to cytoprotective and DNA protective ability of PGP that can help in mucosal layer regeneration.
26704999	9	12	theme	inhibition	1264:1273	arg1	assay					1275:1279	growth inhibition assay	1257:1279	growth inhibition assay followed by scanning electron microscopic studies suggesting that PGP is effective in curbing the growth of H. pylori, which is responsible for ∼70% of gastric ulcer/cancer incidences	1257:1463	Further, PGP was also effective in inhibiting Helicobacter pylori as per growth inhibition assay followed by scanning electron microscopic studies suggesting that PGP is effective in curbing the growth of H. pylori, which is responsible for ∼70% of gastric ulcer/cancer incidences.
26704999	7	13	from	recovery	921:928	arg1	index					897:901	ulcer index	891:901	ulcer index	891:901	About 84% reduction in ulcer index; enhanced mucosal recovery, normalization of H(+), K(+)-ATPase, antioxidant and antioxidant enzymes substantiated the antiulcer potentials of PGP.
26704999	1	14	from	potato	103:108	arg1	properties					61:70	pylori inhibitory properties	43:70	pylori inhibitory properties of pectic polysaccharides from potato	43:108	pylori inhibitory properties of pectic polysaccharides from potato.
26704999	1	14	from	potato	103:108	arg1	polysaccharides					82:96	pectic polysaccharides	75:96	pectic polysaccharides from potato	75:108	pylori inhibitory properties of pectic polysaccharides from potato.
26704999	5	15	theme	IC50	753:756	arg1	μg/mL					763:767	IC50 19.3 μg/mL	753:767	IC50 19.3 μg/mL	753:767	PGP exhibited potent H(+), K(+)-ATPase inhibitory activity (IC50 420 μg/mL) in vitro as opposed to lansoprazole, a known antiulcer drug with IC50 19.3 μg/mL.
26704999	4	16	theme	uronic	545:550	arg1	acid					552:555	uronic acid	545:555	uronic acid (17%)	545:561	Data indicated that PGP possessed sugar composition of rhamnose (2%), arabinose (3%), mannose (3%), galactose (94%) and uronic acid (17%) confirming that PGP thus isolated is a galactan.
26704999	4	16	theme	uronic	545:550	arg1	%					560:560	17%	558:560	17%	558:560	Data indicated that PGP possessed sugar composition of rhamnose (2%), arabinose (3%), mannose (3%), galactose (94%) and uronic acid (17%) confirming that PGP thus isolated is a galactan.
26704999	3	17	theme	Polysaccharide	403:416	arg1	effect					377:382	anti-ulcer effect	366:382	anti-ulcer effect of Potato Galactan Polysaccharide (PGP)	366:422	The present study describes the isolation of galactans and confirmation of the same by sugar analysis and determination of anti-ulcer effect of Potato Galactan Polysaccharide (PGP).
26704999	7	18	theme	H	948:948	arg1	recovery					921:928	enhanced mucosal recovery	904:928	enhanced mucosal recovery	904:928	About 84% reduction in ulcer index; enhanced mucosal recovery, normalization of H(+), K(+)-ATPase, antioxidant and antioxidant enzymes substantiated the antiulcer potentials of PGP.
26704999	7	18	theme	H	948:948	arg1	normalization					931:943	normalization	931:943	normalization of H(+), K(+)-ATPase	931:964	About 84% reduction in ulcer index; enhanced mucosal recovery, normalization of H(+), K(+)-ATPase, antioxidant and antioxidant enzymes substantiated the antiulcer potentials of PGP.
26704999	7	18	theme	H	948:948	arg1	reduction					878:886	About 84% reduction	868:886	About 84% reduction in ulcer index	868:901	About 84% reduction in ulcer index; enhanced mucosal recovery, normalization of H(+), K(+)-ATPase, antioxidant and antioxidant enzymes substantiated the antiulcer potentials of PGP.
26704999	7	18	theme	H	948:948	arg1	antioxidant					967:977	antioxidant	967:977	antioxidant	967:977	About 84% reduction in ulcer index; enhanced mucosal recovery, normalization of H(+), K(+)-ATPase, antioxidant and antioxidant enzymes substantiated the antiulcer potentials of PGP.
26704999	7	18	theme	H	948:948	arg1	enzymes					995:1001	antioxidant enzymes	983:1001	antioxidant enzymes	983:1001	About 84% reduction in ulcer index; enhanced mucosal recovery, normalization of H(+), K(+)-ATPase, antioxidant and antioxidant enzymes substantiated the antiulcer potentials of PGP.
26704999	5	19	theme	potent	626:631	arg1	-ATPase					643:649	K(+)-ATPase	639:649	K(+)-ATPase	639:649	PGP exhibited potent H(+), K(+)-ATPase inhibitory activity (IC50 420 μg/mL) in vitro as opposed to lansoprazole, a known antiulcer drug with IC50 19.3 μg/mL.
26704999	5	19	theme	potent	626:631	arg1	H					633:633	potent H	626:633	potent H	626:633	PGP exhibited potent H(+), K(+)-ATPase inhibitory activity (IC50 420 μg/mL) in vitro as opposed to lansoprazole, a known antiulcer drug with IC50 19.3 μg/mL.
26704999	7	20	from	antioxidant	967:977	arg1	index					897:901	ulcer index	891:901	ulcer index	891:901	About 84% reduction in ulcer index; enhanced mucosal recovery, normalization of H(+), K(+)-ATPase, antioxidant and antioxidant enzymes substantiated the antiulcer potentials of PGP.
26704999	9	21	dep	H.	1389:1390	arg1	responsible					1409:1419	responsible	1409:1419	responsible	1409:1419	Further, PGP was also effective in inhibiting Helicobacter pylori as per growth inhibition assay followed by scanning electron microscopic studies suggesting that PGP is effective in curbing the growth of H. pylori, which is responsible for ∼70% of gastric ulcer/cancer incidences.
26704999	9	21	dep	H.	1389:1390	arg1	pylori					1392:1397	H. pylori, which is responsible for ∼70% of gastric ulcer/cancer incidences	1389:1463	pylori	1392:1397	Further, PGP was also effective in inhibiting Helicobacter pylori as per growth inhibition assay followed by scanning electron microscopic studies suggesting that PGP is effective in curbing the growth of H. pylori, which is responsible for ∼70% of gastric ulcer/cancer incidences.
26704999	6	22	theme	ulcer	847:851	arg1	model					853:857	ethanol stress induced gastric ulcer model	816:857	ethanol stress induced gastric ulcer model	816:857	The antiulcer potency of PGP was evaluated in ethanol stress induced gastric ulcer model in vivo.
26704999	7	23	from	normalization	931:943	arg1	index					897:901	ulcer index	891:901	ulcer index	891:901	About 84% reduction in ulcer index; enhanced mucosal recovery, normalization of H(+), K(+)-ATPase, antioxidant and antioxidant enzymes substantiated the antiulcer potentials of PGP.
26704999	8	24	theme	cytoprotective	1090:1103	arg1	ability					1124:1130	cytoprotective and DNA protective ability	1090:1130	ability	1124:1130	Mucosal recovery could be attributed to cytoprotective and DNA protective ability of PGP that can help in mucosal layer regeneration.
26704999	6	25	theme	gastric	839:845	arg1	model					853:857	ethanol stress induced gastric ulcer model	816:857	ethanol stress induced gastric ulcer model	816:857	The antiulcer potency of PGP was evaluated in ethanol stress induced gastric ulcer model in vivo.
26704999	3	26	theme	Potato	387:392	arg1	Polysaccharide					403:416	Potato Galactan Polysaccharide	387:416	Potato Galactan Polysaccharide (PGP)	387:422	The present study describes the isolation of galactans and confirmation of the same by sugar analysis and determination of anti-ulcer effect of Potato Galactan Polysaccharide (PGP).
26704999	3	26	theme	Potato	387:392	arg1	PGP					419:421	PGP	419:421	PGP	419:421	The present study describes the isolation of galactans and confirmation of the same by sugar analysis and determination of anti-ulcer effect of Potato Galactan Polysaccharide (PGP).
26704999	7	27	dep	%	876:876	arg1	84					874:875	84	874:875	84	874:875	About 84% reduction in ulcer index; enhanced mucosal recovery, normalization of H(+), K(+)-ATPase, antioxidant and antioxidant enzymes substantiated the antiulcer potentials of PGP.
26704999	4	28	theme	arabinose	495:503	arg1	composition					465:475	sugar composition	459:475	sugar composition of rhamnose (2%), arabinose (3%), mannose (3%), galactose (94%) and uronic acid (17%)	459:561	Data indicated that PGP possessed sugar composition of rhamnose (2%), arabinose (3%), mannose (3%), galactose (94%) and uronic acid (17%) confirming that PGP thus isolated is a galactan.
26704999	1	29	from	properties	61:70	arg1	potato					103:108	potato	103:108	potato	103:108	pylori inhibitory properties of pectic polysaccharides from potato.
26704999	9	30	theme	scanning	1293:1300	arg1	studies					1323:1329	scanning electron microscopic studies	1293:1329	scanning electron microscopic studies suggesting that PGP is effective in curbing the growth of H. pylori, which is responsible for ∼70% of gastric ulcer/cancer incidences	1293:1463	Further, PGP was also effective in inhibiting Helicobacter pylori as per growth inhibition assay followed by scanning electron microscopic studies suggesting that PGP is effective in curbing the growth of H. pylori, which is responsible for ∼70% of gastric ulcer/cancer incidences.
26704999	3	31	theme	Galactan	394:401	arg1	Polysaccharide					403:416	Potato Galactan Polysaccharide	387:416	Potato Galactan Polysaccharide (PGP)	387:422	The present study describes the isolation of galactans and confirmation of the same by sugar analysis and determination of anti-ulcer effect of Potato Galactan Polysaccharide (PGP).
26704999	3	31	theme	Galactan	394:401	arg1	PGP					419:421	PGP	419:421	PGP	419:421	The present study describes the isolation of galactans and confirmation of the same by sugar analysis and determination of anti-ulcer effect of Potato Galactan Polysaccharide (PGP).
26704999	3	32	theme	sugar	330:334	arg1	analysis					336:343	sugar analysis	330:343	sugar analysis	330:343	The present study describes the isolation of galactans and confirmation of the same by sugar analysis and determination of anti-ulcer effect of Potato Galactan Polysaccharide (PGP).
26704999	7	33	from	enzymes	995:1001	arg1	index					897:901	ulcer index	891:901	ulcer index	891:901	About 84% reduction in ulcer index; enhanced mucosal recovery, normalization of H(+), K(+)-ATPase, antioxidant and antioxidant enzymes substantiated the antiulcer potentials of PGP.
26704999	1	34	theme	pylori	43:48	arg1	properties					61:70	pylori inhibitory properties	43:70	pylori inhibitory properties of pectic polysaccharides from potato	43:108	pylori inhibitory properties of pectic polysaccharides from potato.
26704999	4	35	theme	galactose	525:533	arg1	composition					465:475	sugar composition	459:475	sugar composition of rhamnose (2%), arabinose (3%), mannose (3%), galactose (94%) and uronic acid (17%)	459:561	Data indicated that PGP possessed sugar composition of rhamnose (2%), arabinose (3%), mannose (3%), galactose (94%) and uronic acid (17%) confirming that PGP thus isolated is a galactan.
26704999	7	36	dep	reduction	878:886	arg1	%					876:876	%	876:876	%	876:876	About 84% reduction in ulcer index; enhanced mucosal recovery, normalization of H(+), K(+)-ATPase, antioxidant and antioxidant enzymes substantiated the antiulcer potentials of PGP.
26704999	3	37	theme	present	247:253	arg1	study					255:259	The present study	243:259	The present study	243:259	The present study describes the isolation of galactans and confirmation of the same by sugar analysis and determination of anti-ulcer effect of Potato Galactan Polysaccharide (PGP).
26704999	8	38	theme	layer	1164:1168	arg1	regeneration					1170:1181	mucosal layer regeneration	1156:1181	mucosal layer regeneration	1156:1181	Mucosal recovery could be attributed to cytoprotective and DNA protective ability of PGP that can help in mucosal layer regeneration.
26704999	1	39	theme	inhibitory	50:59	arg1	properties					61:70	pylori inhibitory properties	43:70	pylori inhibitory properties of pectic polysaccharides from potato	43:108	pylori inhibitory properties of pectic polysaccharides from potato.
26704999	7	40	theme	enhanced	904:911	arg1	recovery					921:928	enhanced mucosal recovery	904:928	enhanced mucosal recovery	904:928	About 84% reduction in ulcer index; enhanced mucosal recovery, normalization of H(+), K(+)-ATPase, antioxidant and antioxidant enzymes substantiated the antiulcer potentials of PGP.
26704999	6	41	theme	ethanol	816:822	arg1	model					853:857	ethanol stress induced gastric ulcer model	816:857	ethanol stress induced gastric ulcer model	816:857	The antiulcer potency of PGP was evaluated in ethanol stress induced gastric ulcer model in vivo.
26704999	3	42	theme	effect	377:382	arg1	confirmation					302:313	confirmation	302:313	confirmation of the same by sugar analysis	302:343	The present study describes the isolation of galactans and confirmation of the same by sugar analysis and determination of anti-ulcer effect of Potato Galactan Polysaccharide (PGP).
26704999	3	42	theme	effect	377:382	arg1	determination					349:361	determination	349:361	determination of anti-ulcer effect of Potato Galactan Polysaccharide (PGP)	349:422	The present study describes the isolation of galactans and confirmation of the same by sugar analysis and determination of anti-ulcer effect of Potato Galactan Polysaccharide (PGP).
26704999	3	42	theme	effect	377:382	arg1	isolation					275:283	the isolation	271:283	the isolation of galactans	271:296	The present study describes the isolation of galactans and confirmation of the same by sugar analysis and determination of anti-ulcer effect of Potato Galactan Polysaccharide (PGP).
26704999	8	43	theme	mucosal	1156:1162	arg1	regeneration					1170:1181	mucosal layer regeneration	1156:1181	mucosal layer regeneration	1156:1181	Mucosal recovery could be attributed to cytoprotective and DNA protective ability of PGP that can help in mucosal layer regeneration.
26704999	6	44	theme	induced	831:837	arg1	model					853:857	ethanol stress induced gastric ulcer model	816:857	ethanol stress induced gastric ulcer model	816:857	The antiulcer potency of PGP was evaluated in ethanol stress induced gastric ulcer model in vivo.
26704999	9	45	theme	gastric	1433:1439	arg1	incidences					1454:1463	gastric ulcer/cancer incidences	1433:1463	gastric ulcer/cancer incidences	1433:1463	Further, PGP was also effective in inhibiting Helicobacter pylori as per growth inhibition assay followed by scanning electron microscopic studies suggesting that PGP is effective in curbing the growth of H. pylori, which is responsible for ∼70% of gastric ulcer/cancer incidences.
26704999	5	46	theme	H	633:633	arg1	μg/mL					681:685	IC50 420 μg/mL	672:685	IC50 420 μg/mL	672:685	PGP exhibited potent H(+), K(+)-ATPase inhibitory activity (IC50 420 μg/mL) in vitro as opposed to lansoprazole, a known antiulcer drug with IC50 19.3 μg/mL.
26704999	5	46	theme	H	633:633	arg1	activity					662:669	potent H(+), K(+)-ATPase inhibitory activity	626:669	potent H(+), K(+)-ATPase inhibitory activity (IC50 420 μg/mL)	626:686	PGP exhibited potent H(+), K(+)-ATPase inhibitory activity (IC50 420 μg/mL) in vitro as opposed to lansoprazole, a known antiulcer drug with IC50 19.3 μg/mL.
26704999	5	47	theme	IC50	672:675	arg1	μg/mL					681:685	IC50 420 μg/mL	672:685	IC50 420 μg/mL	672:685	PGP exhibited potent H(+), K(+)-ATPase inhibitory activity (IC50 420 μg/mL) in vitro as opposed to lansoprazole, a known antiulcer drug with IC50 19.3 μg/mL.
26704999	5	47	theme	IC50	672:675	arg1	activity					662:669	potent H(+), K(+)-ATPase inhibitory activity	626:669	potent H(+), K(+)-ATPase inhibitory activity (IC50 420 μg/mL)	626:686	PGP exhibited potent H(+), K(+)-ATPase inhibitory activity (IC50 420 μg/mL) in vitro as opposed to lansoprazole, a known antiulcer drug with IC50 19.3 μg/mL.
26704999	5	48	theme	K	639:639	arg1	-ATPase					643:649	K(+)-ATPase	639:649	K(+)-ATPase	639:649	PGP exhibited potent H(+), K(+)-ATPase inhibitory activity (IC50 420 μg/mL) in vitro as opposed to lansoprazole, a known antiulcer drug with IC50 19.3 μg/mL.
26704999	5	48	theme	K	639:639	arg1	H					633:633	potent H	626:633	potent H	626:633	PGP exhibited potent H(+), K(+)-ATPase inhibitory activity (IC50 420 μg/mL) in vitro as opposed to lansoprazole, a known antiulcer drug with IC50 19.3 μg/mL.
26704999	5	49	theme	inhibitory	651:660	arg1	μg/mL					681:685	IC50 420 μg/mL	672:685	IC50 420 μg/mL	672:685	PGP exhibited potent H(+), K(+)-ATPase inhibitory activity (IC50 420 μg/mL) in vitro as opposed to lansoprazole, a known antiulcer drug with IC50 19.3 μg/mL.
26704999	5	49	theme	inhibitory	651:660	arg1	activity					662:669	potent H(+), K(+)-ATPase inhibitory activity	626:669	potent H(+), K(+)-ATPase inhibitory activity (IC50 420 μg/mL)	626:686	PGP exhibited potent H(+), K(+)-ATPase inhibitory activity (IC50 420 μg/mL) in vitro as opposed to lansoprazole, a known antiulcer drug with IC50 19.3 μg/mL.
26704999	5	50	with	drug	743:746	arg1	μg/mL					763:767	IC50 19.3 μg/mL	753:767	IC50 19.3 μg/mL	753:767	PGP exhibited potent H(+), K(+)-ATPase inhibitory activity (IC50 420 μg/mL) in vitro as opposed to lansoprazole, a known antiulcer drug with IC50 19.3 μg/mL.
26704999	8	51	theme	PGP	1135:1137	arg1	ability					1124:1130	cytoprotective and DNA protective ability	1090:1130	ability	1124:1130	Mucosal recovery could be attributed to cytoprotective and DNA protective ability of PGP that can help in mucosal layer regeneration.
26704999	7	52	theme	antioxidant	983:993	arg1	enzymes					995:1001	antioxidant enzymes	983:1001	antioxidant enzymes	983:1001	About 84% reduction in ulcer index; enhanced mucosal recovery, normalization of H(+), K(+)-ATPase, antioxidant and antioxidant enzymes substantiated the antiulcer potentials of PGP.
26704999	3	53	theme	anti-ulcer	366:375	arg1	effect					377:382	anti-ulcer effect	366:382	anti-ulcer effect of Potato Galactan Polysaccharide (PGP)	366:422	The present study describes the isolation of galactans and confirmation of the same by sugar analysis and determination of anti-ulcer effect of Potato Galactan Polysaccharide (PGP).
26704999	7	54	from	reduction	878:886	arg1	index					897:901	ulcer index	891:901	ulcer index	891:901	About 84% reduction in ulcer index; enhanced mucosal recovery, normalization of H(+), K(+)-ATPase, antioxidant and antioxidant enzymes substantiated the antiulcer potentials of PGP.
26704999	7	55	theme	PGP	1045:1047	arg1	potentials					1031:1040	the antiulcer potentials	1017:1040	the antiulcer potentials of PGP	1017:1047	About 84% reduction in ulcer index; enhanced mucosal recovery, normalization of H(+), K(+)-ATPase, antioxidant and antioxidant enzymes substantiated the antiulcer potentials of PGP.
26704999	2	56	theme	biological	164:173	arg1	polymers					175:182	biological polymers	164:182	biological polymers	164:182	Polysaccharide is one among the important classes of biological polymers that is reported to exhibit disease preventive properties.
26704999	2	57	theme	polymers	175:182	arg1	classes					153:159	the important classes	139:159	the important classes of biological polymers	139:182	Polysaccharide is one among the important classes of biological polymers that is reported to exhibit disease preventive properties.
26704999	4	58	contain	possessed	449:457	arg1	PGP					445:447	PGP	445:447	PGP	445:447	Data indicated that PGP possessed sugar composition of rhamnose (2%), arabinose (3%), mannose (3%), galactose (94%) and uronic acid (17%) confirming that PGP thus isolated is a galactan.
26704999	4	58	contain	possessed	449:457	arg2	composition					465:475	sugar composition	459:475	sugar composition of rhamnose (2%), arabinose (3%), mannose (3%), galactose (94%) and uronic acid (17%)	459:561	Data indicated that PGP possessed sugar composition of rhamnose (2%), arabinose (3%), mannose (3%), galactose (94%) and uronic acid (17%) confirming that PGP thus isolated is a galactan.
26704999	7	59	theme	mucosal	913:919	arg1	recovery					921:928	enhanced mucosal recovery	904:928	enhanced mucosal recovery	904:928	About 84% reduction in ulcer index; enhanced mucosal recovery, normalization of H(+), K(+)-ATPase, antioxidant and antioxidant enzymes substantiated the antiulcer potentials of PGP.
26704999	9	60	theme	ulcer/cancer	1441:1452	arg1	incidences					1454:1463	gastric ulcer/cancer incidences	1433:1463	gastric ulcer/cancer incidences	1433:1463	Further, PGP was also effective in inhibiting Helicobacter pylori as per growth inhibition assay followed by scanning electron microscopic studies suggesting that PGP is effective in curbing the growth of H. pylori, which is responsible for ∼70% of gastric ulcer/cancer incidences.
26704999	0	61	dep	H	22:22	arg1	K					28:28	K	28:28	K	28:28	Gastro protective and H(+), K(+)-ATPase/H.
26704999	0	61	dep	H	22:22	arg1	-ATPase/H					32:40	-ATPase/H	32:40	-ATPase/H	32:40	Gastro protective and H(+), K(+)-ATPase/H.
26704999	2	62	theme	preventive	220:229	arg1	properties					231:240	disease preventive properties	212:240	disease preventive properties	212:240	Polysaccharide is one among the important classes of biological polymers that is reported to exhibit disease preventive properties.
26704999	8	63	theme	protective	1113:1122	arg1	ability					1124:1130	cytoprotective and DNA protective ability	1090:1130	ability	1124:1130	Mucosal recovery could be attributed to cytoprotective and DNA protective ability of PGP that can help in mucosal layer regeneration.
26704999	1	64	theme	pectic	75:80	arg1	polysaccharides					82:96	pectic polysaccharides	75:96	pectic polysaccharides from potato	75:108	pylori inhibitory properties of pectic polysaccharides from potato.
26704999	5	65	theme	known	727:731	arg1	drug					743:746	a known antiulcer drug	725:746	a known antiulcer drug with IC50 19.3 μg/mL	725:767	PGP exhibited potent H(+), K(+)-ATPase inhibitory activity (IC50 420 μg/mL) in vitro as opposed to lansoprazole, a known antiulcer drug with IC50 19.3 μg/mL.
26704999	5	65	theme	known	727:731	arg1	lansoprazole					711:722	lansoprazole	711:722	lansoprazole	711:722	PGP exhibited potent H(+), K(+)-ATPase inhibitory activity (IC50 420 μg/mL) in vitro as opposed to lansoprazole, a known antiulcer drug with IC50 19.3 μg/mL.
26704999	9	66	theme	incidences	1454:1463	arg1	%					1428:1428	∼70%	1425:1428	∼70% of gastric ulcer/cancer incidences	1425:1463	Further, PGP was also effective in inhibiting Helicobacter pylori as per growth inhibition assay followed by scanning electron microscopic studies suggesting that PGP is effective in curbing the growth of H. pylori, which is responsible for ∼70% of gastric ulcer/cancer incidences.
26704999	9	66	theme	incidences	1454:1463	arg1	incidences					1454:1463	gastric ulcer/cancer incidences	1433:1463	gastric ulcer/cancer incidences	1433:1463	Further, PGP was also effective in inhibiting Helicobacter pylori as per growth inhibition assay followed by scanning electron microscopic studies suggesting that PGP is effective in curbing the growth of H. pylori, which is responsible for ∼70% of gastric ulcer/cancer incidences.
26704999	2	67	theme	disease	212:218	arg1	properties					231:240	disease preventive properties	212:240	disease preventive properties	212:240	Polysaccharide is one among the important classes of biological polymers that is reported to exhibit disease preventive properties.
26704999	1	68	theme	polysaccharides	82:96	arg1	properties					61:70	pylori inhibitory properties	43:70	pylori inhibitory properties of pectic polysaccharides from potato	43:108	pylori inhibitory properties of pectic polysaccharides from potato.
26704999	6	69	theme	PGP	795:797	arg1	potency					784:790	The antiulcer potency	770:790	The antiulcer potency of PGP	770:797	The antiulcer potency of PGP was evaluated in ethanol stress induced gastric ulcer model in vivo.
26704999	7	70	theme	K	954:954	arg1	-ATPase					958:964	K(+)-ATPase	954:964	K(+)-ATPase	954:964	About 84% reduction in ulcer index; enhanced mucosal recovery, normalization of H(+), K(+)-ATPase, antioxidant and antioxidant enzymes substantiated the antiulcer potentials of PGP.
26704999	7	70	theme	K	954:954	arg1	H					948:948	H(+)	948:951	H	948:948	About 84% reduction in ulcer index; enhanced mucosal recovery, normalization of H(+), K(+)-ATPase, antioxidant and antioxidant enzymes substantiated the antiulcer potentials of PGP.
26704999	2	71	theme	important	143:151	arg1	classes					153:159	the important classes	139:159	the important classes of biological polymers	139:182	Polysaccharide is one among the important classes of biological polymers that is reported to exhibit disease preventive properties.
26704999	6	72	theme	stress	824:829	arg1	model					853:857	ethanol stress induced gastric ulcer model	816:857	ethanol stress induced gastric ulcer model	816:857	The antiulcer potency of PGP was evaluated in ethanol stress induced gastric ulcer model in vivo.
26704999	9	73	theme	Helicobacter	1230:1241	arg1	pylori					1243:1248	Helicobacter pylori	1230:1248	Helicobacter pylori	1230:1248	Further, PGP was also effective in inhibiting Helicobacter pylori as per growth inhibition assay followed by scanning electron microscopic studies suggesting that PGP is effective in curbing the growth of H. pylori, which is responsible for ∼70% of gastric ulcer/cancer incidences.
26704999	7	74	theme	antiulcer	1021:1029	arg1	potentials					1031:1040	the antiulcer potentials	1017:1040	the antiulcer potentials of PGP	1017:1047	About 84% reduction in ulcer index; enhanced mucosal recovery, normalization of H(+), K(+)-ATPase, antioxidant and antioxidant enzymes substantiated the antiulcer potentials of PGP.
26704999	7	75	theme	ulcer	891:895	arg1	index					897:901	ulcer index	891:901	ulcer index	891:901	About 84% reduction in ulcer index; enhanced mucosal recovery, normalization of H(+), K(+)-ATPase, antioxidant and antioxidant enzymes substantiated the antiulcer potentials of PGP.
26704999	9	76	theme	electron	1302:1309	arg1	studies					1323:1329	scanning electron microscopic studies	1293:1329	scanning electron microscopic studies suggesting that PGP is effective in curbing the growth of H. pylori, which is responsible for ∼70% of gastric ulcer/cancer incidences	1293:1463	Further, PGP was also effective in inhibiting Helicobacter pylori as per growth inhibition assay followed by scanning electron microscopic studies suggesting that PGP is effective in curbing the growth of H. pylori, which is responsible for ∼70% of gastric ulcer/cancer incidences.
26704999	4	77	theme	acid	552:555	arg1	composition					465:475	sugar composition	459:475	sugar composition of rhamnose (2%), arabinose (3%), mannose (3%), galactose (94%) and uronic acid (17%)	459:561	Data indicated that PGP possessed sugar composition of rhamnose (2%), arabinose (3%), mannose (3%), galactose (94%) and uronic acid (17%) confirming that PGP thus isolated is a galactan.
27261756	11	0	theme	red	1483:1485	arg1	degradation					1444:1454	the photocatalytic degradation	1425:1454	the photocatalytic degradation of methyl orange and congo red	1425:1485	Kinetic study results showed that the photocatalytic degradation of methyl orange and congo red could be explained by Langmuir-Hinshelwood model.
27261756	9	1	theme	efficient	1173:1181	arg1	capacity					1191:1198	an efficient removal capacity	1170:1198	an efficient removal capacity of 95.76%	1170:1208	On the other hand, for the congo red the composite having higher chitosan and Na-titanate showed an efficient removal capacity of 95.76%.
27261756	1	2	theme	casting	210:216	arg1	method					218:223	solution casting method	201:223	solution casting method	201:223	Chitosan/PVA/Na-titanate/TiO2 composite was synthesized by solution casting method.
27261756	6	3	theme	adsorption	704:713	arg1	behavior					715:722	The adsorption behavior	700:722	The adsorption behavior of the composites	700:740	The adsorption behavior of the composites were described by pseudo-second-order kinetic model and Lagergren-first-order model for methyl orange and congo red, respectively.
27261756	12	4	theme	dye	1638:1640	arg1	degradation					1642:1652	dye degradation	1638:1652	dye degradation	1638:1652	Thus, chitosan/PVA/Na-titanate/TiO2 possesses efficient adsorptivity and photocatalytic property for dye degradation.
27261756	12	5	theme	efficient	1583:1591	arg1	adsorptivity					1593:1604	efficient adsorptivity	1583:1604	efficient adsorptivity	1583:1604	Thus, chitosan/PVA/Na-titanate/TiO2 possesses efficient adsorptivity and photocatalytic property for dye degradation.
27261756	12	6	theme	photocatalytic	1610:1623	arg1	property					1625:1632	photocatalytic property	1610:1632	photocatalytic property	1610:1632	Thus, chitosan/PVA/Na-titanate/TiO2 possesses efficient adsorptivity and photocatalytic property for dye degradation.
27261756	0	7	theme	casting	126:132	arg1	method					134:139	solution casting method	117:139	solution casting method	117:139	Adsorption and photocatalytic degradation of anionic dyes on Chitosan/PVA/Na-Titanate/TiO2 composites synthesized by solution casting method.
27261756	0	8	from	Adsorption	0:9	arg1	composites					91:100	Chitosan/PVA/Na-Titanate/TiO2 composites	61:100	Chitosan/PVA/Na-Titanate/TiO2 composites	61:100	Adsorption and photocatalytic degradation of anionic dyes on Chitosan/PVA/Na-Titanate/TiO2 composites synthesized by solution casting method.
27261756	8	9	theme	TiO2	1061:1064	arg1	phase					1066:1070	crystalline TiO2 phase	1049:1070	crystalline TiO2 phase	1049:1070	99.9% of methyl orange dye was removed by the composite having higher weightage of chitosan and crystalline TiO2 phase.
27261756	9	10	theme	higher	1131:1136	arg1	chitosan					1138:1145	higher chitosan	1131:1145	higher chitosan	1131:1145	On the other hand, for the congo red the composite having higher chitosan and Na-titanate showed an efficient removal capacity of 95.76%.
27261756	10	11	theme	orange	1271:1276	arg1	backbone					1252:1259	the molecular backbone	1238:1259	the molecular backbone of methyl orange and congo red	1238:1290	UV-vis results showed that the molecular backbone of methyl orange and congo red was almost destroyed when equilibrium was obtained, and the decolorization rate was reaching 100%.
27261756	0	12	theme	solution	117:124	arg1	method					134:139	solution casting method	117:139	solution casting method	117:139	Adsorption and photocatalytic degradation of anionic dyes on Chitosan/PVA/Na-Titanate/TiO2 composites synthesized by solution casting method.
27261756	4	13	from	loss	596:599	arg1	medium					611:616	acidic medium	604:616	acidic medium	604:616	However, the composite structure was deteriorated with considerable weight loss in acidic medium.
27261756	5	14	theme	adsorption	683:692	arg1	test					694:697	the adsorption test	679:697	the adsorption test	679:697	Two anionic dyes, methyl orange and congo red were used for the adsorption test.
27261756	11	15	theme	Langmuir-Hinshelwood	1509:1528	arg1	model					1530:1534	Langmuir-Hinshelwood model	1509:1534	Langmuir-Hinshelwood model	1509:1534	Kinetic study results showed that the photocatalytic degradation of methyl orange and congo red could be explained by Langmuir-Hinshelwood model.
27261756	5	16	used	used	670:673	arg2	orange					644:649	methyl orange	637:649	methyl orange	637:649	Two anionic dyes, methyl orange and congo red were used for the adsorption test.
27261756	5	16	used	used	670:673	arg2	dyes					631:634	Two anionic dyes	619:634	Two anionic dyes	619:634	Two anionic dyes, methyl orange and congo red were used for the adsorption test.
27261756	5	16	used	used	670:673	arg2	red					661:663	red	661:663	red	661:663	Two anionic dyes, methyl orange and congo red were used for the adsorption test.
27261756	10	17	theme	methyl	1264:1269	arg1	orange					1271:1276	methyl orange	1264:1276	methyl orange	1264:1276	UV-vis results showed that the molecular backbone of methyl orange and congo red was almost destroyed when equilibrium was obtained, and the decolorization rate was reaching 100%.
27261756	11	18	theme	orange	1466:1471	arg1	degradation					1444:1454	the photocatalytic degradation	1425:1454	the photocatalytic degradation of methyl orange and congo red	1425:1485	Kinetic study results showed that the photocatalytic degradation of methyl orange and congo red could be explained by Langmuir-Hinshelwood model.
27261756	8	19	theme	methyl	962:967	arg1	dye					976:978	methyl orange dye	962:978	methyl orange dye	962:978	99.9% of methyl orange dye was removed by the composite having higher weightage of chitosan and crystalline TiO2 phase.
27261756	10	20	theme	decolorization	1352:1365	arg1	rate					1367:1370	the decolorization rate	1348:1370	the decolorization rate	1348:1370	UV-vis results showed that the molecular backbone of methyl orange and congo red was almost destroyed when equilibrium was obtained, and the decolorization rate was reaching 100%.
27261756	2	21	theme	Electron	341:348	arg1	Microscopy					350:359	Field Emission Scanning Electron Microscopy	317:359	Field Emission Scanning Electron Microscopy	317:359	The composite was analyzed via Fourier Transform Infrared Spectroscopy, X-ray diffraction, Field Emission Scanning Electron Microscopy, Thermal gravimetric analysis and water stability test.
27261756	2	21	theme	Electron	341:348	arg1	Fourier					257:263	Fourier	257:263	Fourier Transform Infrared Spectroscopy	257:295	The composite was analyzed via Fourier Transform Infrared Spectroscopy, X-ray diffraction, Field Emission Scanning Electron Microscopy, Thermal gravimetric analysis and water stability test.
27261756	6	22	theme	Lagergren-first-order	798:818	arg1	model					820:824	Lagergren-first-order model	798:824	Lagergren-first-order model	798:824	The adsorption behavior of the composites were described by pseudo-second-order kinetic model and Lagergren-first-order model for methyl orange and congo red, respectively.
27261756	4	23	theme	weight	589:594	arg1	loss					596:599	considerable weight loss	576:599	considerable weight loss in acidic medium	576:616	However, the composite structure was deteriorated with considerable weight loss in acidic medium.
27261756	2	24	theme	Scanning	332:339	arg1	Microscopy					350:359	Field Emission Scanning Electron Microscopy	317:359	Field Emission Scanning Electron Microscopy	317:359	The composite was analyzed via Fourier Transform Infrared Spectroscopy, X-ray diffraction, Field Emission Scanning Electron Microscopy, Thermal gravimetric analysis and water stability test.
27261756	2	24	theme	Scanning	332:339	arg1	Fourier					257:263	Fourier	257:263	Fourier Transform Infrared Spectroscopy	257:295	The composite was analyzed via Fourier Transform Infrared Spectroscopy, X-ray diffraction, Field Emission Scanning Electron Microscopy, Thermal gravimetric analysis and water stability test.
27261756	8	25	theme	dye	976:978	arg1	dye					976:978	methyl orange dye	962:978	methyl orange dye	962:978	99.9% of methyl orange dye was removed by the composite having higher weightage of chitosan and crystalline TiO2 phase.
27261756	8	25	theme	dye	976:978	arg1	%					957:957	99.9%	953:957	99.9% of methyl orange dye	953:978	99.9% of methyl orange dye was removed by the composite having higher weightage of chitosan and crystalline TiO2 phase.
27261756	3	26	theme	crystallinity	464:476	arg1	decrease					452:459	decrease	452:459	decrease of crystallinity	452:476	Incorporation of Na-titanate shown decrease of crystallinity for chitosan but increase water stability.
27261756	4	27	theme	considerable	576:587	arg1	loss					596:599	considerable weight loss	576:599	considerable weight loss in acidic medium	576:616	However, the composite structure was deteriorated with considerable weight loss in acidic medium.
27261756	8	28	theme	orange	969:974	arg1	dye					976:978	methyl orange dye	962:978	methyl orange dye	962:978	99.9% of methyl orange dye was removed by the composite having higher weightage of chitosan and crystalline TiO2 phase.
27261756	11	29	theme	study	1399:1403	arg1	results					1405:1411	Kinetic study results	1391:1411	Kinetic study results	1391:1411	Kinetic study results showed that the photocatalytic degradation of methyl orange and congo red could be explained by Langmuir-Hinshelwood model.
27261756	11	30	theme	methyl	1459:1464	arg1	orange					1466:1471	methyl orange	1459:1471	methyl orange	1459:1471	Kinetic study results showed that the photocatalytic degradation of methyl orange and congo red could be explained by Langmuir-Hinshelwood model.
27261756	6	31	theme	kinetic	780:786	arg1	model					788:792	pseudo-second-order kinetic model	760:792	pseudo-second-order kinetic model	760:792	The adsorption behavior of the composites were described by pseudo-second-order kinetic model and Lagergren-first-order model for methyl orange and congo red, respectively.
27261756	11	32	theme	photocatalytic	1429:1442	arg1	degradation					1444:1454	the photocatalytic degradation	1425:1454	the photocatalytic degradation of methyl orange and congo red	1425:1485	Kinetic study results showed that the photocatalytic degradation of methyl orange and congo red could be explained by Langmuir-Hinshelwood model.
27261756	2	33	theme	gravimetric	370:380	arg1	Fourier					257:263	Fourier	257:263	Fourier Transform Infrared Spectroscopy	257:295	The composite was analyzed via Fourier Transform Infrared Spectroscopy, X-ray diffraction, Field Emission Scanning Electron Microscopy, Thermal gravimetric analysis and water stability test.
27261756	2	33	theme	gravimetric	370:380	arg1	analysis					382:389	Thermal gravimetric analysis	362:389	Thermal gravimetric analysis	362:389	The composite was analyzed via Fourier Transform Infrared Spectroscopy, X-ray diffraction, Field Emission Scanning Electron Microscopy, Thermal gravimetric analysis and water stability test.
27261756	6	34	theme	pseudo-second-order	760:778	arg1	model					788:792	pseudo-second-order kinetic model	760:792	pseudo-second-order kinetic model	760:792	The adsorption behavior of the composites were described by pseudo-second-order kinetic model and Lagergren-first-order model for methyl orange and congo red, respectively.
27261756	0	35	theme	photocatalytic	15:28	arg1	degradation					30:40	photocatalytic degradation	15:40	photocatalytic degradation	15:40	Adsorption and photocatalytic degradation of anionic dyes on Chitosan/PVA/Na-Titanate/TiO2 composites synthesized by solution casting method.
27261756	4	36	theme	composite	534:542	arg1	structure					544:552	the composite structure	530:552	the composite structure	530:552	However, the composite structure was deteriorated with considerable weight loss in acidic medium.
27261756	2	37	theme	Thermal	362:368	arg1	Fourier					257:263	Fourier	257:263	Fourier Transform Infrared Spectroscopy	257:295	The composite was analyzed via Fourier Transform Infrared Spectroscopy, X-ray diffraction, Field Emission Scanning Electron Microscopy, Thermal gravimetric analysis and water stability test.
27261756	2	37	theme	Thermal	362:368	arg1	analysis					382:389	Thermal gravimetric analysis	362:389	Thermal gravimetric analysis	362:389	The composite was analyzed via Fourier Transform Infrared Spectroscopy, X-ray diffraction, Field Emission Scanning Electron Microscopy, Thermal gravimetric analysis and water stability test.
27261756	9	38	theme	removal	1183:1189	arg1	capacity					1191:1198	an efficient removal capacity	1170:1198	an efficient removal capacity of 95.76%	1170:1208	On the other hand, for the congo red the composite having higher chitosan and Na-titanate showed an efficient removal capacity of 95.76%.
27261756	7	39	theme	promising	922:930	arg1	rate					947:950	a promising decolorization rate	920:950	a promising decolorization rate	920:950	For methyl orange, adsorption was started with a promising decolorization rate.
27261756	0	40	theme	anionic	45:51	arg1	dyes					53:56	anionic dyes	45:56	anionic dyes	45:56	Adsorption and photocatalytic degradation of anionic dyes on Chitosan/PVA/Na-Titanate/TiO2 composites synthesized by solution casting method.
27261756	2	41	theme	X-ray	298:302	arg1	Fourier					257:263	Fourier	257:263	Fourier Transform Infrared Spectroscopy	257:295	The composite was analyzed via Fourier Transform Infrared Spectroscopy, X-ray diffraction, Field Emission Scanning Electron Microscopy, Thermal gravimetric analysis and water stability test.
27261756	2	41	theme	X-ray	298:302	arg1	diffraction					304:314	X-ray diffraction	298:314	X-ray diffraction	298:314	The composite was analyzed via Fourier Transform Infrared Spectroscopy, X-ray diffraction, Field Emission Scanning Electron Microscopy, Thermal gravimetric analysis and water stability test.
27261756	10	42	theme	red	1288:1290	arg1	backbone					1252:1259	the molecular backbone	1238:1259	the molecular backbone of methyl orange and congo red	1238:1290	UV-vis results showed that the molecular backbone of methyl orange and congo red was almost destroyed when equilibrium was obtained, and the decolorization rate was reaching 100%.
27261756	3	43	theme	water	504:508	arg1	stability					510:518	water stability	504:518	water stability	504:518	Incorporation of Na-titanate shown decrease of crystallinity for chitosan but increase water stability.
27261756	7	44	theme	methyl	877:882	arg1	orange					884:889	methyl orange	877:889	methyl orange	877:889	For methyl orange, adsorption was started with a promising decolorization rate.
27261756	9	45	theme	other	1080:1084	arg1	hand					1086:1089	the other hand	1076:1089	the other hand	1076:1089	On the other hand, for the congo red the composite having higher chitosan and Na-titanate showed an efficient removal capacity of 95.76%.
27261756	10	46	theme	UV-vis	1211:1216	arg1	results					1218:1224	UV-vis results	1211:1224	UV-vis results	1211:1224	UV-vis results showed that the molecular backbone of methyl orange and congo red was almost destroyed when equilibrium was obtained, and the decolorization rate was reaching 100%.
27261756	3	47	theme	Na-titanate	434:444	arg1	Incorporation					417:429	Incorporation	417:429	Incorporation of Na-titanate	417:444	Incorporation of Na-titanate shown decrease of crystallinity for chitosan but increase water stability.
27261756	7	48	theme	decolorization	932:945	arg1	rate					947:950	a promising decolorization rate	920:950	a promising decolorization rate	920:950	For methyl orange, adsorption was started with a promising decolorization rate.
27261756	9	49	contain	having	1124:1129	arg1	composite					1114:1122	the composite	1110:1122	the composite having higher chitosan and Na-titanate	1110:1161	On the other hand, for the congo red the composite having higher chitosan and Na-titanate showed an efficient removal capacity of 95.76%.
27261756	9	49	contain	having	1124:1129	arg2	chitosan					1138:1145	higher chitosan	1131:1145	higher chitosan	1131:1145	On the other hand, for the congo red the composite having higher chitosan and Na-titanate showed an efficient removal capacity of 95.76%.
27261756	9	49	contain	having	1124:1129	arg2	Na-titanate					1151:1161	Na-titanate	1151:1161	Na-titanate	1151:1161	On the other hand, for the congo red the composite having higher chitosan and Na-titanate showed an efficient removal capacity of 95.76%.
27261756	5	50	theme	methyl	637:642	arg1	dyes					631:634	Two anionic dyes	619:634	Two anionic dyes	619:634	Two anionic dyes, methyl orange and congo red were used for the adsorption test.
27261756	5	50	theme	methyl	637:642	arg1	orange					644:649	methyl orange	637:649	methyl orange	637:649	Two anionic dyes, methyl orange and congo red were used for the adsorption test.
27261756	1	51	theme	Chitosan/PVA/Na-titanate/TiO2	142:170	arg1	composite					172:180	Chitosan/PVA/Na-titanate/TiO2 composite	142:180	Chitosan/PVA/Na-titanate/TiO2 composite	142:180	Chitosan/PVA/Na-titanate/TiO2 composite was synthesized by solution casting method.
27261756	0	52	theme	dyes	53:56	arg1	Adsorption					0:9	Adsorption	0:9	Adsorption	0:9	Adsorption and photocatalytic degradation of anionic dyes on Chitosan/PVA/Na-Titanate/TiO2 composites synthesized by solution casting method.
27261756	0	52	theme	dyes	53:56	arg1	degradation					30:40	photocatalytic degradation	15:40	photocatalytic degradation	15:40	Adsorption and photocatalytic degradation of anionic dyes on Chitosan/PVA/Na-Titanate/TiO2 composites synthesized by solution casting method.
27261756	2	53	theme	Infrared	275:282	arg1	Spectroscopy					284:295	Infrared Spectroscopy	275:295	Infrared Spectroscopy	275:295	The composite was analyzed via Fourier Transform Infrared Spectroscopy, X-ray diffraction, Field Emission Scanning Electron Microscopy, Thermal gravimetric analysis and water stability test.
27261756	9	54	theme	%	1208:1208	arg1	capacity					1191:1198	an efficient removal capacity	1170:1198	an efficient removal capacity of 95.76%	1170:1208	On the other hand, for the congo red the composite having higher chitosan and Na-titanate showed an efficient removal capacity of 95.76%.
27261756	12	55	contain	possesses	1573:1581	arg2	property					1625:1632	photocatalytic property	1610:1632	photocatalytic property	1610:1632	Thus, chitosan/PVA/Na-titanate/TiO2 possesses efficient adsorptivity and photocatalytic property for dye degradation.
27261756	12	55	contain	possesses	1573:1581	arg1	chitosan/PVA/Na-titanate/TiO2					1543:1571	chitosan/PVA/Na-titanate/TiO2	1543:1571	chitosan/PVA/Na-titanate/TiO2	1543:1571	Thus, chitosan/PVA/Na-titanate/TiO2 possesses efficient adsorptivity and photocatalytic property for dye degradation.
27261756	12	55	contain	possesses	1573:1581	arg2	adsorptivity					1593:1604	efficient adsorptivity	1583:1604	efficient adsorptivity	1583:1604	Thus, chitosan/PVA/Na-titanate/TiO2 possesses efficient adsorptivity and photocatalytic property for dye degradation.
27261756	8	56	contain	having	1009:1014	arg1	composite					999:1007	the composite	995:1007	the composite having higher weightage of chitosan and crystalline TiO2 phase	995:1070	99.9% of methyl orange dye was removed by the composite having higher weightage of chitosan and crystalline TiO2 phase.
27261756	8	56	contain	having	1009:1014	arg2	weightage					1023:1031	higher weightage	1016:1031	higher weightage of chitosan	1016:1043	99.9% of methyl orange dye was removed by the composite having higher weightage of chitosan and crystalline TiO2 phase.
27261756	8	56	contain	having	1009:1014	arg2	phase					1066:1070	crystalline TiO2 phase	1049:1070	crystalline TiO2 phase	1049:1070	99.9% of methyl orange dye was removed by the composite having higher weightage of chitosan and crystalline TiO2 phase.
27261756	8	57	theme	crystalline	1049:1059	arg1	phase					1066:1070	crystalline TiO2 phase	1049:1070	crystalline TiO2 phase	1049:1070	99.9% of methyl orange dye was removed by the composite having higher weightage of chitosan and crystalline TiO2 phase.
27261756	2	58	theme	Emission	323:330	arg1	Microscopy					350:359	Field Emission Scanning Electron Microscopy	317:359	Field Emission Scanning Electron Microscopy	317:359	The composite was analyzed via Fourier Transform Infrared Spectroscopy, X-ray diffraction, Field Emission Scanning Electron Microscopy, Thermal gravimetric analysis and water stability test.
27261756	2	58	theme	Emission	323:330	arg1	Fourier					257:263	Fourier	257:263	Fourier Transform Infrared Spectroscopy	257:295	The composite was analyzed via Fourier Transform Infrared Spectroscopy, X-ray diffraction, Field Emission Scanning Electron Microscopy, Thermal gravimetric analysis and water stability test.
27261756	8	59	theme	higher	1016:1021	arg1	weightage					1023:1031	higher weightage	1016:1031	higher weightage of chitosan	1016:1043	99.9% of methyl orange dye was removed by the composite having higher weightage of chitosan and crystalline TiO2 phase.
27261756	2	60	theme	stability	401:409	arg1	test					411:414	water stability test	395:414	water stability test	395:414	The composite was analyzed via Fourier Transform Infrared Spectroscopy, X-ray diffraction, Field Emission Scanning Electron Microscopy, Thermal gravimetric analysis and water stability test.
27261756	2	60	theme	stability	401:409	arg1	Fourier					257:263	Fourier	257:263	Fourier Transform Infrared Spectroscopy	257:295	The composite was analyzed via Fourier Transform Infrared Spectroscopy, X-ray diffraction, Field Emission Scanning Electron Microscopy, Thermal gravimetric analysis and water stability test.
27261756	6	61	theme	methyl	830:835	arg1	orange					837:842	methyl orange	830:842	methyl orange	830:842	The adsorption behavior of the composites were described by pseudo-second-order kinetic model and Lagergren-first-order model for methyl orange and congo red, respectively.
27261756	11	62	theme	Kinetic	1391:1397	arg1	results					1405:1411	Kinetic study results	1391:1411	Kinetic study results	1391:1411	Kinetic study results showed that the photocatalytic degradation of methyl orange and congo red could be explained by Langmuir-Hinshelwood model.
27261756	4	63	theme	acidic	604:609	arg1	medium					611:616	acidic medium	604:616	acidic medium	604:616	However, the composite structure was deteriorated with considerable weight loss in acidic medium.
27261756	2	64	theme	Field	317:321	arg1	Microscopy					350:359	Field Emission Scanning Electron Microscopy	317:359	Field Emission Scanning Electron Microscopy	317:359	The composite was analyzed via Fourier Transform Infrared Spectroscopy, X-ray diffraction, Field Emission Scanning Electron Microscopy, Thermal gravimetric analysis and water stability test.
27261756	2	64	theme	Field	317:321	arg1	Fourier					257:263	Fourier	257:263	Fourier Transform Infrared Spectroscopy	257:295	The composite was analyzed via Fourier Transform Infrared Spectroscopy, X-ray diffraction, Field Emission Scanning Electron Microscopy, Thermal gravimetric analysis and water stability test.
27261756	8	65	theme	chitosan	1036:1043	arg1	weightage					1023:1031	higher weightage	1016:1031	higher weightage of chitosan	1016:1043	99.9% of methyl orange dye was removed by the composite having higher weightage of chitosan and crystalline TiO2 phase.
27261756	8	65	theme	chitosan	1036:1043	arg1	phase					1066:1070	crystalline TiO2 phase	1049:1070	crystalline TiO2 phase	1049:1070	99.9% of methyl orange dye was removed by the composite having higher weightage of chitosan and crystalline TiO2 phase.
27261756	2	66	theme	water	395:399	arg1	test					411:414	water stability test	395:414	water stability test	395:414	The composite was analyzed via Fourier Transform Infrared Spectroscopy, X-ray diffraction, Field Emission Scanning Electron Microscopy, Thermal gravimetric analysis and water stability test.
27261756	2	66	theme	water	395:399	arg1	Fourier					257:263	Fourier	257:263	Fourier Transform Infrared Spectroscopy	257:295	The composite was analyzed via Fourier Transform Infrared Spectroscopy, X-ray diffraction, Field Emission Scanning Electron Microscopy, Thermal gravimetric analysis and water stability test.
27261756	6	67	theme	composites	731:740	arg1	behavior					715:722	The adsorption behavior	700:722	The adsorption behavior of the composites	700:740	The adsorption behavior of the composites were described by pseudo-second-order kinetic model and Lagergren-first-order model for methyl orange and congo red, respectively.
27261756	10	68	theme	molecular	1242:1250	arg1	backbone					1252:1259	the molecular backbone	1238:1259	the molecular backbone of methyl orange and congo red	1238:1290	UV-vis results showed that the molecular backbone of methyl orange and congo red was almost destroyed when equilibrium was obtained, and the decolorization rate was reaching 100%.
27261756	0	69	theme	Chitosan/PVA/Na-Titanate/TiO2	61:89	arg1	composites					91:100	Chitosan/PVA/Na-Titanate/TiO2 composites	61:100	Chitosan/PVA/Na-Titanate/TiO2 composites	61:100	Adsorption and photocatalytic degradation of anionic dyes on Chitosan/PVA/Na-Titanate/TiO2 composites synthesized by solution casting method.
27261756	0	70	from	degradation	30:40	arg1	composites					91:100	Chitosan/PVA/Na-Titanate/TiO2 composites	61:100	Chitosan/PVA/Na-Titanate/TiO2 composites	61:100	Adsorption and photocatalytic degradation of anionic dyes on Chitosan/PVA/Na-Titanate/TiO2 composites synthesized by solution casting method.
27261756	5	71	theme	anionic	623:629	arg1	dyes					631:634	Two anionic dyes	619:634	Two anionic dyes	619:634	Two anionic dyes, methyl orange and congo red were used for the adsorption test.
27261756	5	71	theme	anionic	623:629	arg1	orange					644:649	methyl orange	637:649	methyl orange	637:649	Two anionic dyes, methyl orange and congo red were used for the adsorption test.
27261756	5	71	theme	anionic	623:629	arg1	red					661:663	red	661:663	red	661:663	Two anionic dyes, methyl orange and congo red were used for the adsorption test.
27261756	1	72	theme	solution	201:208	arg1	method					218:223	solution casting method	201:223	solution casting method	201:223	Chitosan/PVA/Na-titanate/TiO2 composite was synthesized by solution casting method.
27261756	2	73	dep	Fourier	257:263	arg1	Transform					265:273	Transform	265:273	Transform Infrared Spectroscopy	265:295	The composite was analyzed via Fourier Transform Infrared Spectroscopy, X-ray diffraction, Field Emission Scanning Electron Microscopy, Thermal gravimetric analysis and water stability test.
27309944	0	0	theme	adhesive	92:99	arg1	Mefp-1					110:115	Mefp-1	110:115	Mefp-1	110:115	Controllable degradation of medical magnesium by electrodeposited composite films of mussel adhesive protein (Mefp-1) and chitosan.
27309944	0	0	theme	adhesive	92:99	arg1	protein					101:107	mussel adhesive protein	85:107	mussel adhesive protein (Mefp-1)	85:116	Controllable degradation of medical magnesium by electrodeposited composite films of mussel adhesive protein (Mefp-1) and chitosan.
27309944	2	1	theme	electron	475:482	arg1	microscope					484:493	scanning electron microscope	466:493	scanning electron microscope (SEM)	466:499	The compositions and structures of the films were characterized by atomic force microscope (AFM), scanning electron microscope (SEM) and infrared reflection absorption spectroscopy (IRAS).
27309944	2	1	theme	electron	475:482	arg1	SEM					496:498	SEM	496:498	SEM	496:498	The compositions and structures of the films were characterized by atomic force microscope (AFM), scanning electron microscope (SEM) and infrared reflection absorption spectroscopy (IRAS).
27309944	1	2	theme	degradation	147:157	arg1	rate					159:162	the degradation rate	143:162	the degradation rate of medical magnesium in body fluid environment	143:209	To control the degradation rate of medical magnesium in body fluid environment, biocompatible films composed of Mussel Adhesive Protein (Mefp-1) and chitosan were electrodeposited on magnesium surface in cathodic constant current mode.
27309944	1	3	theme	Mussel	244:249	arg1	Mefp-1					269:274	Mefp-1	269:274	Mefp-1	269:274	To control the degradation rate of medical magnesium in body fluid environment, biocompatible films composed of Mussel Adhesive Protein (Mefp-1) and chitosan were electrodeposited on magnesium surface in cathodic constant current mode.
27309944	1	3	theme	Mussel	244:249	arg1	Protein					260:266	Mussel Adhesive Protein	244:266	Mussel Adhesive Protein (Mefp-1)	244:275	To control the degradation rate of medical magnesium in body fluid environment, biocompatible films composed of Mussel Adhesive Protein (Mefp-1) and chitosan were electrodeposited on magnesium surface in cathodic constant current mode.
27309944	0	4	theme	mussel	85:90	arg1	Mefp-1					110:115	Mefp-1	110:115	Mefp-1	110:115	Controllable degradation of medical magnesium by electrodeposited composite films of mussel adhesive protein (Mefp-1) and chitosan.
27309944	0	4	theme	mussel	85:90	arg1	protein					101:107	mussel adhesive protein	85:107	mussel adhesive protein (Mefp-1)	85:116	Controllable degradation of medical magnesium by electrodeposited composite films of mussel adhesive protein (Mefp-1) and chitosan.
27309944	1	5	theme	cathodic	336:343	arg1	mode					362:365	cathodic constant current mode	336:365	cathodic constant current mode	336:365	To control the degradation rate of medical magnesium in body fluid environment, biocompatible films composed of Mussel Adhesive Protein (Mefp-1) and chitosan were electrodeposited on magnesium surface in cathodic constant current mode.
27309944	1	6	theme	Adhesive	251:258	arg1	Mefp-1					269:274	Mefp-1	269:274	Mefp-1	269:274	To control the degradation rate of medical magnesium in body fluid environment, biocompatible films composed of Mussel Adhesive Protein (Mefp-1) and chitosan were electrodeposited on magnesium surface in cathodic constant current mode.
27309944	1	6	theme	Adhesive	251:258	arg1	Protein					260:266	Mussel Adhesive Protein	244:266	Mussel Adhesive Protein (Mefp-1)	244:275	To control the degradation rate of medical magnesium in body fluid environment, biocompatible films composed of Mussel Adhesive Protein (Mefp-1) and chitosan were electrodeposited on magnesium surface in cathodic constant current mode.
27309944	2	7	theme	infrared	505:512	arg1	spectroscopy					536:547	infrared reflection absorption spectroscopy	505:547	infrared reflection absorption spectroscopy (IRAS)	505:554	The compositions and structures of the films were characterized by atomic force microscope (AFM), scanning electron microscope (SEM) and infrared reflection absorption spectroscopy (IRAS).
27309944	2	7	theme	infrared	505:512	arg1	IRAS					550:553	IRAS	550:553	IRAS	550:553	The compositions and structures of the films were characterized by atomic force microscope (AFM), scanning electron microscope (SEM) and infrared reflection absorption spectroscopy (IRAS).
27309944	1	8	theme	constant	345:352	arg1	mode					362:365	cathodic constant current mode	336:365	cathodic constant current mode	336:365	To control the degradation rate of medical magnesium in body fluid environment, biocompatible films composed of Mussel Adhesive Protein (Mefp-1) and chitosan were electrodeposited on magnesium surface in cathodic constant current mode.
27309944	1	9	from	rate	159:162	arg1	environment					199:209	body fluid environment	188:209	body fluid environment	188:209	To control the degradation rate of medical magnesium in body fluid environment, biocompatible films composed of Mussel Adhesive Protein (Mefp-1) and chitosan were electrodeposited on magnesium surface in cathodic constant current mode.
27309944	5	10	theme	current	1001:1007	arg1	density					1009:1015	lower corrosion current density	985:1015	lower corrosion current density	985:1015	Compared with either single Mefp-1 or single chitosan film, the composite film of chitosan/Mefp-1/chitosan (CPC (chitosan/Mefp-1/chitosan)) exhibited lower corrosion current density, higher polarization resistance and more homogenous corrosion morphology and thus was able to effectively control the degradation rate of magnesium in simulated body environment.
27309944	6	11	theme	cells	1257:1261	arg1	attachment					1220:1229	active attachment	1213:1229	active attachment	1213:1229	In addition, the active attachment and spreading of MC3T3-E1 cells on the CPC film coated magnesium indicated that the CPC film was significantly able to improve the biocompatibility of the medical magnesium.
27309944	6	11	theme	cells	1257:1261	arg1	spreading					1235:1243	spreading	1235:1243	spreading	1235:1243	In addition, the active attachment and spreading of MC3T3-E1 cells on the CPC film coated magnesium indicated that the CPC film was significantly able to improve the biocompatibility of the medical magnesium.
27309944	0	12	theme	protein	101:107	arg1	films					76:80	electrodeposited composite films	49:80	electrodeposited composite films of mussel adhesive protein (Mefp-1) and chitosan	49:129	Controllable degradation of medical magnesium by electrodeposited composite films of mussel adhesive protein (Mefp-1) and chitosan.
27309944	1	13	theme	current	354:360	arg1	mode					362:365	cathodic constant current mode	336:365	cathodic constant current mode	336:365	To control the degradation rate of medical magnesium in body fluid environment, biocompatible films composed of Mussel Adhesive Protein (Mefp-1) and chitosan were electrodeposited on magnesium surface in cathodic constant current mode.
27309944	6	14	theme	coated	1279:1284	arg1	magnesium					1286:1294	the CPC film coated magnesium	1266:1294	the CPC film coated magnesium	1266:1294	In addition, the active attachment and spreading of MC3T3-E1 cells on the CPC film coated magnesium indicated that the CPC film was significantly able to improve the biocompatibility of the medical magnesium.
27309944	6	15	dep	attachment	1220:1229	arg1	the					1209:1211	the	1209:1211	the	1209:1211	In addition, the active attachment and spreading of MC3T3-E1 cells on the CPC film coated magnesium indicated that the CPC film was significantly able to improve the biocompatibility of the medical magnesium.
27309944	6	16	theme	film	1274:1277	arg1	magnesium					1286:1294	the CPC film coated magnesium	1266:1294	the CPC film coated magnesium	1266:1294	In addition, the active attachment and spreading of MC3T3-E1 cells on the CPC film coated magnesium indicated that the CPC film was significantly able to improve the biocompatibility of the medical magnesium.
27309944	5	17	theme	single	856:861	arg1	Mefp-1					863:868	single Mefp-1	856:868	single Mefp-1	856:868	Compared with either single Mefp-1 or single chitosan film, the composite film of chitosan/Mefp-1/chitosan (CPC (chitosan/Mefp-1/chitosan)) exhibited lower corrosion current density, higher polarization resistance and more homogenous corrosion morphology and thus was able to effectively control the degradation rate of magnesium in simulated body environment.
27309944	5	18	from	rate	1147:1150	arg1	environment					1183:1193	simulated body environment	1168:1193	simulated body environment	1168:1193	Compared with either single Mefp-1 or single chitosan film, the composite film of chitosan/Mefp-1/chitosan (CPC (chitosan/Mefp-1/chitosan)) exhibited lower corrosion current density, higher polarization resistance and more homogenous corrosion morphology and thus was able to effectively control the degradation rate of magnesium in simulated body environment.
27309944	6	19	from	attachment	1220:1229	arg1	magnesium					1286:1294	the CPC film coated magnesium	1266:1294	the CPC film coated magnesium	1266:1294	In addition, the active attachment and spreading of MC3T3-E1 cells on the CPC film coated magnesium indicated that the CPC film was significantly able to improve the biocompatibility of the medical magnesium.
27309944	6	20	theme	CPC	1270:1272	arg1	magnesium					1286:1294	the CPC film coated magnesium	1266:1294	the CPC film coated magnesium	1266:1294	In addition, the active attachment and spreading of MC3T3-E1 cells on the CPC film coated magnesium indicated that the CPC film was significantly able to improve the biocompatibility of the medical magnesium.
27309944	2	21	theme	force	442:446	arg1	AFM					460:462	AFM	460:462	AFM	460:462	The compositions and structures of the films were characterized by atomic force microscope (AFM), scanning electron microscope (SEM) and infrared reflection absorption spectroscopy (IRAS).
27309944	2	21	theme	force	442:446	arg1	microscope					448:457	atomic force microscope	435:457	atomic force microscope (AFM)	435:463	The compositions and structures of the films were characterized by atomic force microscope (AFM), scanning electron microscope (SEM) and infrared reflection absorption spectroscopy (IRAS).
27309944	1	22	theme	medical	167:173	arg1	magnesium					175:183	medical magnesium	167:183	medical magnesium	167:183	To control the degradation rate of medical magnesium in body fluid environment, biocompatible films composed of Mussel Adhesive Protein (Mefp-1) and chitosan were electrodeposited on magnesium surface in cathodic constant current mode.
27309944	2	23	theme	scanning	466:473	arg1	microscope					484:493	scanning electron microscope	466:493	scanning electron microscope (SEM)	466:499	The compositions and structures of the films were characterized by atomic force microscope (AFM), scanning electron microscope (SEM) and infrared reflection absorption spectroscopy (IRAS).
27309944	2	23	theme	scanning	466:473	arg1	SEM					496:498	SEM	496:498	SEM	496:498	The compositions and structures of the films were characterized by atomic force microscope (AFM), scanning electron microscope (SEM) and infrared reflection absorption spectroscopy (IRAS).
27309944	6	24	theme	MC3T3-E1	1248:1255	arg1	cells					1257:1261	MC3T3-E1 cells	1248:1261	MC3T3-E1 cells	1248:1261	In addition, the active attachment and spreading of MC3T3-E1 cells on the CPC film coated magnesium indicated that the CPC film was significantly able to improve the biocompatibility of the medical magnesium.
27309944	1	25	theme	magnesium	175:183	arg1	rate					159:162	the degradation rate	143:162	the degradation rate of medical magnesium in body fluid environment	143:209	To control the degradation rate of medical magnesium in body fluid environment, biocompatible films composed of Mussel Adhesive Protein (Mefp-1) and chitosan were electrodeposited on magnesium surface in cathodic constant current mode.
27309944	0	26	theme	Controllable	0:11	arg1	degradation					13:23	Controllable degradation	0:23	Controllable degradation of medical magnesium by electrodeposited composite films of mussel adhesive protein (Mefp-1) and chitosan.	0:130	Controllable degradation of medical magnesium by electrodeposited composite films of mussel adhesive protein (Mefp-1) and chitosan.
27309944	6	27	attach	attachment	1220:1229	arg2	cells					1257:1261	MC3T3-E1 cells	1248:1261	MC3T3-E1 cells	1248:1261	In addition, the active attachment and spreading of MC3T3-E1 cells on the CPC film coated magnesium indicated that the CPC film was significantly able to improve the biocompatibility of the medical magnesium.
27309944	6	27	attach	attachment	1220:1229	arg3	magnesium					1286:1294	the CPC film coated magnesium	1266:1294	the CPC film coated magnesium	1266:1294	In addition, the active attachment and spreading of MC3T3-E1 cells on the CPC film coated magnesium indicated that the CPC film was significantly able to improve the biocompatibility of the medical magnesium.
27309944	5	28	theme	single	873:878	arg1	film					889:892	single chitosan film	873:892	single chitosan film	873:892	Compared with either single Mefp-1 or single chitosan film, the composite film of chitosan/Mefp-1/chitosan (CPC (chitosan/Mefp-1/chitosan)) exhibited lower corrosion current density, higher polarization resistance and more homogenous corrosion morphology and thus was able to effectively control the degradation rate of magnesium in simulated body environment.
27309944	0	29	theme	medical	28:34	arg1	magnesium					36:44	medical magnesium	28:44	medical magnesium	28:44	Controllable degradation of medical magnesium by electrodeposited composite films of mussel adhesive protein (Mefp-1) and chitosan.
27309944	6	30	theme	magnesium	1394:1402	arg1	biocompatibility					1362:1377	the biocompatibility	1358:1377	the biocompatibility of the medical magnesium	1358:1402	In addition, the active attachment and spreading of MC3T3-E1 cells on the CPC film coated magnesium indicated that the CPC film was significantly able to improve the biocompatibility of the medical magnesium.
27309944	0	31	theme	chitosan	122:129	arg1	films					76:80	electrodeposited composite films	49:80	electrodeposited composite films of mussel adhesive protein (Mefp-1) and chitosan	49:129	Controllable degradation of medical magnesium by electrodeposited composite films of mussel adhesive protein (Mefp-1) and chitosan.
27309944	4	32	theme	protective	818:827	arg1	film					829:832	a protective film	816:832	a protective film	816:832	The results revealed that Mefp-1 and chitosan successfully adhered on the magnesium surface and formed a protective film.
27309944	6	33	theme	medical	1386:1392	arg1	magnesium					1394:1402	the medical magnesium	1382:1402	the medical magnesium	1382:1402	In addition, the active attachment and spreading of MC3T3-E1 cells on the CPC film coated magnesium indicated that the CPC film was significantly able to improve the biocompatibility of the medical magnesium.
27309944	5	34	theme	higher	1018:1023	arg1	resistance					1038:1047	higher polarization resistance	1018:1047	higher polarization resistance	1018:1047	Compared with either single Mefp-1 or single chitosan film, the composite film of chitosan/Mefp-1/chitosan (CPC (chitosan/Mefp-1/chitosan)) exhibited lower corrosion current density, higher polarization resistance and more homogenous corrosion morphology and thus was able to effectively control the degradation rate of magnesium in simulated body environment.
27309944	1	35	theme	body	188:191	arg1	environment					199:209	body fluid environment	188:209	body fluid environment	188:209	To control the degradation rate of medical magnesium in body fluid environment, biocompatible films composed of Mussel Adhesive Protein (Mefp-1) and chitosan were electrodeposited on magnesium surface in cathodic constant current mode.
27309944	3	36	theme	immersion	654:662	arg1	tests					664:668	immersion tests	654:668	immersion tests	654:668	And the corrosion protection performance was investigated using electrochemical measurements and immersion tests in simulated body fluid (Hanks' solution).
27309944	6	37	theme	active	1213:1218	arg1	attachment					1220:1229	active attachment	1213:1229	active attachment	1213:1229	In addition, the active attachment and spreading of MC3T3-E1 cells on the CPC film coated magnesium indicated that the CPC film was significantly able to improve the biocompatibility of the medical magnesium.
27309944	1	38	theme	fluid	193:197	arg1	environment					199:209	body fluid environment	188:209	body fluid environment	188:209	To control the degradation rate of medical magnesium in body fluid environment, biocompatible films composed of Mussel Adhesive Protein (Mefp-1) and chitosan were electrodeposited on magnesium surface in cathodic constant current mode.
27309944	2	39	theme	absorption	525:534	arg1	spectroscopy					536:547	infrared reflection absorption spectroscopy	505:547	infrared reflection absorption spectroscopy (IRAS)	505:554	The compositions and structures of the films were characterized by atomic force microscope (AFM), scanning electron microscope (SEM) and infrared reflection absorption spectroscopy (IRAS).
27309944	2	39	theme	absorption	525:534	arg1	IRAS					550:553	IRAS	550:553	IRAS	550:553	The compositions and structures of the films were characterized by atomic force microscope (AFM), scanning electron microscope (SEM) and infrared reflection absorption spectroscopy (IRAS).
27309944	3	40	theme	protection	575:584	arg1	performance					586:596	the corrosion protection performance	561:596	the corrosion protection performance	561:596	And the corrosion protection performance was investigated using electrochemical measurements and immersion tests in simulated body fluid (Hanks' solution).
27309944	2	41	theme	films	407:411	arg1	structures					389:398	structures	389:398	structures	389:398	The compositions and structures of the films were characterized by atomic force microscope (AFM), scanning electron microscope (SEM) and infrared reflection absorption spectroscopy (IRAS).
27309944	2	41	theme	films	407:411	arg1	compositions					372:383	compositions	372:383	compositions	372:383	The compositions and structures of the films were characterized by atomic force microscope (AFM), scanning electron microscope (SEM) and infrared reflection absorption spectroscopy (IRAS).
27309944	0	42	theme	magnesium	36:44	arg1	degradation					13:23	Controllable degradation	0:23	Controllable degradation of medical magnesium by electrodeposited composite films of mussel adhesive protein (Mefp-1) and chitosan.	0:130	Controllable degradation of medical magnesium by electrodeposited composite films of mussel adhesive protein (Mefp-1) and chitosan.
27309944	2	43	theme	reflection	514:523	arg1	spectroscopy					536:547	infrared reflection absorption spectroscopy	505:547	infrared reflection absorption spectroscopy (IRAS)	505:554	The compositions and structures of the films were characterized by atomic force microscope (AFM), scanning electron microscope (SEM) and infrared reflection absorption spectroscopy (IRAS).
27309944	2	43	theme	reflection	514:523	arg1	IRAS					550:553	IRAS	550:553	IRAS	550:553	The compositions and structures of the films were characterized by atomic force microscope (AFM), scanning electron microscope (SEM) and infrared reflection absorption spectroscopy (IRAS).
27309944	2	44	theme	atomic	435:440	arg1	AFM					460:462	AFM	460:462	AFM	460:462	The compositions and structures of the films were characterized by atomic force microscope (AFM), scanning electron microscope (SEM) and infrared reflection absorption spectroscopy (IRAS).
27309944	2	44	theme	atomic	435:440	arg1	microscope					448:457	atomic force microscope	435:457	atomic force microscope (AFM)	435:463	The compositions and structures of the films were characterized by atomic force microscope (AFM), scanning electron microscope (SEM) and infrared reflection absorption spectroscopy (IRAS).
27309944	0	45	theme	composite	66:74	arg1	films					76:80	electrodeposited composite films	49:80	electrodeposited composite films of mussel adhesive protein (Mefp-1) and chitosan	49:129	Controllable degradation of medical magnesium by electrodeposited composite films of mussel adhesive protein (Mefp-1) and chitosan.
27309944	5	46	theme	simulated	1168:1176	arg1	environment					1183:1193	simulated body environment	1168:1193	simulated body environment	1168:1193	Compared with either single Mefp-1 or single chitosan film, the composite film of chitosan/Mefp-1/chitosan (CPC (chitosan/Mefp-1/chitosan)) exhibited lower corrosion current density, higher polarization resistance and more homogenous corrosion morphology and thus was able to effectively control the degradation rate of magnesium in simulated body environment.
27309944	5	47	theme	homogenous	1058:1067	arg1	morphology					1079:1088	more homogenous corrosion morphology	1053:1088	more homogenous corrosion morphology	1053:1088	Compared with either single Mefp-1 or single chitosan film, the composite film of chitosan/Mefp-1/chitosan (CPC (chitosan/Mefp-1/chitosan)) exhibited lower corrosion current density, higher polarization resistance and more homogenous corrosion morphology and thus was able to effectively control the degradation rate of magnesium in simulated body environment.
27309944	3	48	theme	electrochemical	621:635	arg1	measurements					637:648	electrochemical measurements	621:648	electrochemical measurements	621:648	And the corrosion protection performance was investigated using electrochemical measurements and immersion tests in simulated body fluid (Hanks' solution).
27309944	5	49	theme	composite	899:907	arg1	film					909:912	the composite film	895:912	the composite film of chitosan/Mefp-1/chitosan (CPC (chitosan/Mefp-1/chitosan))	895:973	Compared with either single Mefp-1 or single chitosan film, the composite film of chitosan/Mefp-1/chitosan (CPC (chitosan/Mefp-1/chitosan)) exhibited lower corrosion current density, higher polarization resistance and more homogenous corrosion morphology and thus was able to effectively control the degradation rate of magnesium in simulated body environment.
27309944	5	49	theme	composite	899:907	arg1	able					1103:1106	able	1103:1106	able	1103:1106	Compared with either single Mefp-1 or single chitosan film, the composite film of chitosan/Mefp-1/chitosan (CPC (chitosan/Mefp-1/chitosan)) exhibited lower corrosion current density, higher polarization resistance and more homogenous corrosion morphology and thus was able to effectively control the degradation rate of magnesium in simulated body environment.
27309944	0	50	theme	electrodeposited	49:64	arg1	films					76:80	electrodeposited composite films	49:80	electrodeposited composite films of mussel adhesive protein (Mefp-1) and chitosan	49:129	Controllable degradation of medical magnesium by electrodeposited composite films of mussel adhesive protein (Mefp-1) and chitosan.
27309944	5	51	theme	body	1178:1181	arg1	environment					1183:1193	simulated body environment	1168:1193	simulated body environment	1168:1193	Compared with either single Mefp-1 or single chitosan film, the composite film of chitosan/Mefp-1/chitosan (CPC (chitosan/Mefp-1/chitosan)) exhibited lower corrosion current density, higher polarization resistance and more homogenous corrosion morphology and thus was able to effectively control the degradation rate of magnesium in simulated body environment.
27309944	3	52	theme	body	683:686	arg1	solution					702:709	Hanks' solution	695:709	Hanks' solution	695:709	And the corrosion protection performance was investigated using electrochemical measurements and immersion tests in simulated body fluid (Hanks' solution).
27309944	3	52	theme	body	683:686	arg1	fluid					688:692	simulated body fluid	673:692	simulated body fluid (Hanks' solution)	673:710	And the corrosion protection performance was investigated using electrochemical measurements and immersion tests in simulated body fluid (Hanks' solution).
27309944	3	53	theme	corrosion	565:573	arg1	performance					586:596	the corrosion protection performance	561:596	the corrosion protection performance	561:596	And the corrosion protection performance was investigated using electrochemical measurements and immersion tests in simulated body fluid (Hanks' solution).
27309944	1	54	theme	magnesium	315:323	arg1	surface					325:331	magnesium surface	315:331	magnesium surface	315:331	To control the degradation rate of medical magnesium in body fluid environment, biocompatible films composed of Mussel Adhesive Protein (Mefp-1) and chitosan were electrodeposited on magnesium surface in cathodic constant current mode.
27309944	6	55	theme	CPC	1315:1317	arg1	film					1319:1322	the CPC film	1311:1322	the CPC film	1311:1322	In addition, the active attachment and spreading of MC3T3-E1 cells on the CPC film coated magnesium indicated that the CPC film was significantly able to improve the biocompatibility of the medical magnesium.
27309944	6	55	theme	CPC	1315:1317	arg1	able					1342:1345	able	1342:1345	able	1342:1345	In addition, the active attachment and spreading of MC3T3-E1 cells on the CPC film coated magnesium indicated that the CPC film was significantly able to improve the biocompatibility of the medical magnesium.
27309944	5	56	theme	corrosion	991:999	arg1	density					1009:1015	lower corrosion current density	985:1015	lower corrosion current density	985:1015	Compared with either single Mefp-1 or single chitosan film, the composite film of chitosan/Mefp-1/chitosan (CPC (chitosan/Mefp-1/chitosan)) exhibited lower corrosion current density, higher polarization resistance and more homogenous corrosion morphology and thus was able to effectively control the degradation rate of magnesium in simulated body environment.
27309944	4	57	theme	magnesium	787:795	arg1	surface					797:803	the magnesium surface	783:803	the magnesium surface	783:803	The results revealed that Mefp-1 and chitosan successfully adhered on the magnesium surface and formed a protective film.
27309944	5	58	theme	polarization	1025:1036	arg1	resistance					1038:1047	higher polarization resistance	1018:1047	higher polarization resistance	1018:1047	Compared with either single Mefp-1 or single chitosan film, the composite film of chitosan/Mefp-1/chitosan (CPC (chitosan/Mefp-1/chitosan)) exhibited lower corrosion current density, higher polarization resistance and more homogenous corrosion morphology and thus was able to effectively control the degradation rate of magnesium in simulated body environment.
27309944	6	59	from	spreading	1235:1243	arg1	magnesium					1286:1294	the CPC film coated magnesium	1266:1294	the CPC film coated magnesium	1266:1294	In addition, the active attachment and spreading of MC3T3-E1 cells on the CPC film coated magnesium indicated that the CPC film was significantly able to improve the biocompatibility of the medical magnesium.
27309944	1	60	theme	biocompatible	212:224	arg1	films					226:230	biocompatible films	212:230	biocompatible films composed of Mussel Adhesive Protein (Mefp-1) and chitosan	212:288	To control the degradation rate of medical magnesium in body fluid environment, biocompatible films composed of Mussel Adhesive Protein (Mefp-1) and chitosan were electrodeposited on magnesium surface in cathodic constant current mode.
27309944	5	61	theme	lower	985:989	arg1	density					1009:1015	lower corrosion current density	985:1015	lower corrosion current density	985:1015	Compared with either single Mefp-1 or single chitosan film, the composite film of chitosan/Mefp-1/chitosan (CPC (chitosan/Mefp-1/chitosan)) exhibited lower corrosion current density, higher polarization resistance and more homogenous corrosion morphology and thus was able to effectively control the degradation rate of magnesium in simulated body environment.
27309944	5	62	theme	corrosion	1069:1077	arg1	morphology					1079:1088	more homogenous corrosion morphology	1053:1088	more homogenous corrosion morphology	1053:1088	Compared with either single Mefp-1 or single chitosan film, the composite film of chitosan/Mefp-1/chitosan (CPC (chitosan/Mefp-1/chitosan)) exhibited lower corrosion current density, higher polarization resistance and more homogenous corrosion morphology and thus was able to effectively control the degradation rate of magnesium in simulated body environment.
27309944	2	63	dep	compositions	372:383	arg1	The					368:370	The	368:370	The	368:370	The compositions and structures of the films were characterized by atomic force microscope (AFM), scanning electron microscope (SEM) and infrared reflection absorption spectroscopy (IRAS).
27309944	5	64	theme	chitosan/Mefp-1/chitosan	917:940	arg1	film					909:912	the composite film	895:912	the composite film of chitosan/Mefp-1/chitosan (CPC (chitosan/Mefp-1/chitosan))	895:973	Compared with either single Mefp-1 or single chitosan film, the composite film of chitosan/Mefp-1/chitosan (CPC (chitosan/Mefp-1/chitosan)) exhibited lower corrosion current density, higher polarization resistance and more homogenous corrosion morphology and thus was able to effectively control the degradation rate of magnesium in simulated body environment.
27309944	5	64	theme	chitosan/Mefp-1/chitosan	917:940	arg1	able					1103:1106	able	1103:1106	able	1103:1106	Compared with either single Mefp-1 or single chitosan film, the composite film of chitosan/Mefp-1/chitosan (CPC (chitosan/Mefp-1/chitosan)) exhibited lower corrosion current density, higher polarization resistance and more homogenous corrosion morphology and thus was able to effectively control the degradation rate of magnesium in simulated body environment.
27309944	3	65	theme	simulated	673:681	arg1	solution					702:709	Hanks' solution	695:709	Hanks' solution	695:709	And the corrosion protection performance was investigated using electrochemical measurements and immersion tests in simulated body fluid (Hanks' solution).
27309944	3	65	theme	simulated	673:681	arg1	fluid					688:692	simulated body fluid	673:692	simulated body fluid (Hanks' solution)	673:710	And the corrosion protection performance was investigated using electrochemical measurements and immersion tests in simulated body fluid (Hanks' solution).
27309944	5	66	theme	magnesium	1155:1163	arg1	rate					1147:1150	the degradation rate	1131:1150	the degradation rate of magnesium in simulated body environment	1131:1193	Compared with either single Mefp-1 or single chitosan film, the composite film of chitosan/Mefp-1/chitosan (CPC (chitosan/Mefp-1/chitosan)) exhibited lower corrosion current density, higher polarization resistance and more homogenous corrosion morphology and thus was able to effectively control the degradation rate of magnesium in simulated body environment.
27309944	5	67	theme	chitosan	880:887	arg1	film					889:892	single chitosan film	873:892	single chitosan film	873:892	Compared with either single Mefp-1 or single chitosan film, the composite film of chitosan/Mefp-1/chitosan (CPC (chitosan/Mefp-1/chitosan)) exhibited lower corrosion current density, higher polarization resistance and more homogenous corrosion morphology and thus was able to effectively control the degradation rate of magnesium in simulated body environment.
27309944	5	68	theme	degradation	1135:1145	arg1	rate					1147:1150	the degradation rate	1131:1150	the degradation rate of magnesium in simulated body environment	1131:1193	Compared with either single Mefp-1 or single chitosan film, the composite film of chitosan/Mefp-1/chitosan (CPC (chitosan/Mefp-1/chitosan)) exhibited lower corrosion current density, higher polarization resistance and more homogenous corrosion morphology and thus was able to effectively control the degradation rate of magnesium in simulated body environment.
24474069	9	0	theme	hydrolysis	1499:1508	arg1	efficiency					1510:1519	the hydrolysis efficiency	1495:1519	the hydrolysis efficiency of the starch hydrolysis	1495:1544	These modifications in culture conditions increase the hydrolysis efficiency of the starch hydrolysis, thus providing potentially important gains for biofuel production.
24474069	7	1	theme	CO2-grown	1094:1102	arg1	cells					1104:1108	2% CO2-grown cells	1091:1108	2% CO2-grown cells	1091:1108	The rate of hydrolysis of starch from 2% CO2-grown cells was 1.25 times greater than that from air-grown cells and 2-11 times higher than the rates of hydrolysis of starches from cereal grains.
24474069	7	2	from	cells	1104:1108	arg1	hydrolysis					1065:1074	hydrolysis	1065:1074	hydrolysis of starch from 2% CO2-grown cells	1065:1108	The rate of hydrolysis of starch from 2% CO2-grown cells was 1.25 times greater than that from air-grown cells and 2-11 times higher than the rates of hydrolysis of starches from cereal grains.
24474069	7	2	from	cells	1104:1108	arg1	rate					1057:1060	The rate	1053:1060	The rate of hydrolysis of starch from 2% CO2-grown cells	1053:1108	The rate of hydrolysis of starch from 2% CO2-grown cells was 1.25 times greater than that from air-grown cells and 2-11 times higher than the rates of hydrolysis of starches from cereal grains.
24474069	7	2	from	cells	1104:1108	arg1	times					1119:1123	times	1119:1123	times	1119:1123	The rate of hydrolysis of starch from 2% CO2-grown cells was 1.25 times greater than that from air-grown cells and 2-11 times higher than the rates of hydrolysis of starches from cereal grains.
24474069	9	3	theme	starch	1528:1533	arg1	hydrolysis					1535:1544	the starch hydrolysis	1524:1544	the starch hydrolysis	1524:1544	These modifications in culture conditions increase the hydrolysis efficiency of the starch hydrolysis, thus providing potentially important gains for biofuel production.
24474069	8	4	from	use	1417:1419	arg1	generation					1432:1441	biofuel generation	1424:1441	biofuel generation	1424:1441	These data indicate that culturing C. sorokiniana in elevated CO2 not only increases biomass yield but also improves the structure and composition of starch granules for use in biofuel generation.
24474069	3	5	theme	%	529:529	arg1	CO2					531:533	2% CO2	528:533	2% CO2	528:533	The production of C. sorokiniana biomass, lipid and starch were enhanced when cultures were supplied with 2% CO2.
24474069	8	6	dep	culturing	1272:1280	arg1	improves					1355:1362	improves	1355:1362	culturing C. sorokiniana in elevated CO2 not only increases biomass yield but also improves the structure and composition of starch granules for use in biofuel generation	1272:1441	These data indicate that culturing C. sorokiniana in elevated CO2 not only increases biomass yield but also improves the structure and composition of starch granules for use in biofuel generation.
24474069	8	6	dep	culturing	1272:1280	arg1	increases					1322:1330	increases	1322:1330	culturing C. sorokiniana in elevated CO2 not only increases biomass yield but also improves the structure and composition of starch granules for use in biofuel generation	1272:1441	These data indicate that culturing C. sorokiniana in elevated CO2 not only increases biomass yield but also improves the structure and composition of starch granules for use in biofuel generation.
24474069	9	7	theme	important	1574:1582	arg1	gains					1584:1588	potentially important gains	1562:1588	potentially important gains	1562:1588	These modifications in culture conditions increase the hydrolysis efficiency of the starch hydrolysis, thus providing potentially important gains for biofuel production.
24474069	4	8	theme	2	588:588	arg1	%					589:589	%	589:589	%	589:589	Starch granules from algae grown in ambient air and 2% CO2 were analyzed by transmission electron microscopy (TEM) and scanning electron microscopy (SEM).
24474069	5	9	theme	%	725:725	arg1	CO2					727:729	2% CO2	724:729	2% CO2	724:729	The granules from algae grown in 2% CO2 were disk-shaped and contained mainly stromal starch; granules from cultures grown in ambient air were cup-shaped with primarily pyrenoid starch.
24474069	8	10	theme	elevated	1300:1307	arg1	CO2					1309:1311	elevated CO2	1300:1311	elevated CO2	1300:1311	These data indicate that culturing C. sorokiniana in elevated CO2 not only increases biomass yield but also improves the structure and composition of starch granules for use in biofuel generation.
24474069	5	11	theme	ambient	817:823	arg1	air					825:827	ambient air	817:827	ambient air	817:827	The granules from algae grown in 2% CO2 were disk-shaped and contained mainly stromal starch; granules from cultures grown in ambient air were cup-shaped with primarily pyrenoid starch.
24474069	7	12	from	grains	1239:1244	arg1	starches					1218:1225	starches	1218:1225	starches from cereal grains	1218:1244	The rate of hydrolysis of starch from 2% CO2-grown cells was 1.25 times greater than that from air-grown cells and 2-11 times higher than the rates of hydrolysis of starches from cereal grains.
24474069	7	12	from	grains	1239:1244	arg1	hydrolysis					1204:1213	hydrolysis	1204:1213	hydrolysis of starches from cereal grains	1204:1244	The rate of hydrolysis of starch from 2% CO2-grown cells was 1.25 times greater than that from air-grown cells and 2-11 times higher than the rates of hydrolysis of starches from cereal grains.
24474069	4	13	theme	transmission	612:623	arg1	TEM					646:648	TEM	646:648	TEM	646:648	Starch granules from algae grown in ambient air and 2% CO2 were analyzed by transmission electron microscopy (TEM) and scanning electron microscopy (SEM).
24474069	4	13	theme	transmission	612:623	arg1	microscopy					634:643	transmission electron microscopy	612:643	transmission electron microscopy (TEM)	612:649	Starch granules from algae grown in ambient air and 2% CO2 were analyzed by transmission electron microscopy (TEM) and scanning electron microscopy (SEM).
24474069	7	14	theme	%	1092:1092	arg1	cells					1104:1108	2% CO2-grown cells	1091:1108	2% CO2-grown cells	1091:1108	The rate of hydrolysis of starch from 2% CO2-grown cells was 1.25 times greater than that from air-grown cells and 2-11 times higher than the rates of hydrolysis of starches from cereal grains.
24474069	5	15	from	algae	709:713	arg1	granules					695:702	The granules	691:702	The granules from algae grown in 2% CO2	691:729	The granules from algae grown in 2% CO2 were disk-shaped and contained mainly stromal starch; granules from cultures grown in ambient air were cup-shaped with primarily pyrenoid starch.
24474069	5	15	from	algae	709:713	arg1	disk-shaped					736:746	disk-shaped	736:746	disk-shaped	736:746	The granules from algae grown in 2% CO2 were disk-shaped and contained mainly stromal starch; granules from cultures grown in ambient air were cup-shaped with primarily pyrenoid starch.
24474069	6	16	theme	2	910:910	arg1	%					911:911	%	911:911	%	911:911	The granules from cells grown in 2% CO2 had a higher proportion of the accumulated starch as the highly branched, amylopectin glucan than did granules from cells grown in air.
24474069	0	17	theme	algal	59:63	arg1	starch					65:70	algal starch	59:70	algal starch	59:70	The impact of elevated CO2 concentration on the quality of algal starch as a potential biofuel feedstock.
24474069	5	18	from	cultures	799:806	arg1	granules					785:792	granules	785:792	granules from cultures grown in ambient air	785:827	The granules from algae grown in 2% CO2 were disk-shaped and contained mainly stromal starch; granules from cultures grown in ambient air were cup-shaped with primarily pyrenoid starch.
24474069	1	19	theme	important	140:148	arg1	producers					150:158	important producers	140:158	important producers	140:158	Cultured microalgae are viewed as important producers of lipids and polysaccharides, both of which are precursor molecules for the production of biofuels.
24474069	1	19	theme	important	140:148	arg1	microalgae					115:124	Cultured microalgae	106:124	Cultured microalgae	106:124	Cultured microalgae are viewed as important producers of lipids and polysaccharides, both of which are precursor molecules for the production of biofuels.
24474069	7	20	theme	starch	1079:1084	arg1	hydrolysis					1065:1074	hydrolysis	1065:1074	hydrolysis of starch from 2% CO2-grown cells	1065:1108	The rate of hydrolysis of starch from 2% CO2-grown cells was 1.25 times greater than that from air-grown cells and 2-11 times higher than the rates of hydrolysis of starches from cereal grains.
24474069	3	21	dep	sorokiniana	443:453	arg1	biomass					455:461	biomass	455:461	biomass	455:461	The production of C. sorokiniana biomass, lipid and starch were enhanced when cultures were supplied with 2% CO2.
24474069	3	21	dep	sorokiniana	443:453	arg1	starch					474:479	starch	474:479	starch	474:479	The production of C. sorokiniana biomass, lipid and starch were enhanced when cultures were supplied with 2% CO2.
24474069	3	21	dep	sorokiniana	443:453	arg1	lipid					464:468	lipid	464:468	lipid	464:468	The production of C. sorokiniana biomass, lipid and starch were enhanced when cultures were supplied with 2% CO2.
24474069	6	22	from	cells	895:899	arg1	granules					881:888	The granules	877:888	The granules from cells grown in 2% CO2	877:915	The granules from cells grown in 2% CO2 had a higher proportion of the accumulated starch as the highly branched, amylopectin glucan than did granules from cells grown in air.
24474069	2	23	theme	starch	365:370	arg1	production					351:360	Chlorella sorokiniana production	329:360	Chlorella sorokiniana production of starch	329:370	This study addressed the impact of elevated carbon dioxide (CO2) on Chlorella sorokiniana production of starch and on several properties of the starch produced.
24474069	0	24	theme	potential	77:85	arg1	feedstock					95:103	a potential biofuel feedstock	75:103	a potential biofuel feedstock	75:103	The impact of elevated CO2 concentration on the quality of algal starch as a potential biofuel feedstock.
24474069	6	25	theme	starch	960:965	arg1	proportion					930:939	a higher proportion	921:939	a higher proportion of the accumulated starch	921:965	The granules from cells grown in 2% CO2 had a higher proportion of the accumulated starch as the highly branched, amylopectin glucan than did granules from cells grown in air.
24474069	6	25	theme	starch	960:965	arg1	glucan					1003:1008	the highly branched, amylopectin glucan	970:1008	the highly branched, amylopectin glucan than did granules from cells grown in air	970:1050	The granules from cells grown in 2% CO2 had a higher proportion of the accumulated starch as the highly branched, amylopectin glucan than did granules from cells grown in air.
24474069	4	26	theme	electron	664:671	arg1	microscopy					673:682	scanning electron microscopy	655:682	scanning electron microscopy (SEM)	655:688	Starch granules from algae grown in ambient air and 2% CO2 were analyzed by transmission electron microscopy (TEM) and scanning electron microscopy (SEM).
24474069	4	26	theme	electron	664:671	arg1	SEM					685:687	SEM	685:687	SEM	685:687	Starch granules from algae grown in ambient air and 2% CO2 were analyzed by transmission electron microscopy (TEM) and scanning electron microscopy (SEM).
24474069	5	27	theme	2	724:724	arg1	%					725:725	%	725:725	%	725:725	The granules from algae grown in 2% CO2 were disk-shaped and contained mainly stromal starch; granules from cultures grown in ambient air were cup-shaped with primarily pyrenoid starch.
24474069	1	28	theme	biofuels	251:258	arg1	production					237:246	the production	233:246	the production of biofuels	233:258	Cultured microalgae are viewed as important producers of lipids and polysaccharides, both of which are precursor molecules for the production of biofuels.
24474069	9	29	from	modifications	1450:1462	arg1	conditions					1475:1484	culture conditions	1467:1484	culture conditions	1467:1484	These modifications in culture conditions increase the hydrolysis efficiency of the starch hydrolysis, thus providing potentially important gains for biofuel production.
24474069	5	30	theme	pyrenoid	860:867	arg1	starch					869:874	primarily pyrenoid starch	850:874	primarily pyrenoid starch	850:874	The granules from algae grown in 2% CO2 were disk-shaped and contained mainly stromal starch; granules from cultures grown in ambient air were cup-shaped with primarily pyrenoid starch.
24474069	8	31	theme	granules	1404:1411	arg1	structure					1368:1376	structure	1368:1376	structure	1368:1376	These data indicate that culturing C. sorokiniana in elevated CO2 not only increases biomass yield but also improves the structure and composition of starch granules for use in biofuel generation.
24474069	8	31	theme	granules	1404:1411	arg1	composition					1382:1392	composition	1382:1392	composition	1382:1392	These data indicate that culturing C. sorokiniana in elevated CO2 not only increases biomass yield but also improves the structure and composition of starch granules for use in biofuel generation.
24474069	6	32	from	cells	1033:1037	arg1	granules					1019:1026	granules	1019:1026	granules from cells grown in air	1019:1050	The granules from cells grown in 2% CO2 had a higher proportion of the accumulated starch as the highly branched, amylopectin glucan than did granules from cells grown in air.
24474069	0	33	theme	CO2	23:25	arg1	concentration					27:39	elevated CO2 concentration	14:39	elevated CO2 concentration	14:39	The impact of elevated CO2 concentration on the quality of algal starch as a potential biofuel feedstock.
24474069	7	34	theme	air-grown	1148:1156	arg1	cells					1158:1162	air-grown cells	1148:1162	air-grown cells	1148:1162	The rate of hydrolysis of starch from 2% CO2-grown cells was 1.25 times greater than that from air-grown cells and 2-11 times higher than the rates of hydrolysis of starches from cereal grains.
24474069	8	35	theme	biofuel	1424:1430	arg1	generation					1432:1441	biofuel generation	1424:1441	biofuel generation	1424:1441	These data indicate that culturing C. sorokiniana in elevated CO2 not only increases biomass yield but also improves the structure and composition of starch granules for use in biofuel generation.
24474069	6	36	dep	glucan	1003:1008	arg1	did					1015:1017	did	1015:1017	did	1015:1017	The granules from cells grown in 2% CO2 had a higher proportion of the accumulated starch as the highly branched, amylopectin glucan than did granules from cells grown in air.
24474069	3	37	theme	C.	440:441	arg1	sorokiniana					443:453	C. sorokiniana biomass, lipid and starch	440:479	C. sorokiniana biomass, lipid and starch	440:479	The production of C. sorokiniana biomass, lipid and starch were enhanced when cultures were supplied with 2% CO2.
24474069	6	38	theme	branched	981:988	arg1	proportion					930:939	a higher proportion	921:939	a higher proportion of the accumulated starch	921:965	The granules from cells grown in 2% CO2 had a higher proportion of the accumulated starch as the highly branched, amylopectin glucan than did granules from cells grown in air.
24474069	6	38	theme	branched	981:988	arg1	glucan					1003:1008	the highly branched, amylopectin glucan	970:1008	the highly branched, amylopectin glucan than did granules from cells grown in air	970:1050	The granules from cells grown in 2% CO2 had a higher proportion of the accumulated starch as the highly branched, amylopectin glucan than did granules from cells grown in air.
24474069	6	39	dep	did	1015:1017	arg1	than					1010:1013	than	1010:1013	than	1010:1013	The granules from cells grown in 2% CO2 had a higher proportion of the accumulated starch as the highly branched, amylopectin glucan than did granules from cells grown in air.
24474069	2	40	theme	Chlorella	329:337	arg1	production					351:360	Chlorella sorokiniana production	329:360	Chlorella sorokiniana production of starch	329:370	This study addressed the impact of elevated carbon dioxide (CO2) on Chlorella sorokiniana production of starch and on several properties of the starch produced.
24474069	8	41	dep	structure	1368:1376	arg1	the					1364:1366	the	1364:1366	the	1364:1366	These data indicate that culturing C. sorokiniana in elevated CO2 not only increases biomass yield but also improves the structure and composition of starch granules for use in biofuel generation.
24474069	7	42	theme	1.25	1114:1117	arg1	rate					1057:1060	The rate	1053:1060	The rate of hydrolysis of starch from 2% CO2-grown cells	1053:1108	The rate of hydrolysis of starch from 2% CO2-grown cells was 1.25 times greater than that from air-grown cells and 2-11 times higher than the rates of hydrolysis of starches from cereal grains.
24474069	7	42	theme	1.25	1114:1117	arg1	times					1119:1123	times	1119:1123	times	1119:1123	The rate of hydrolysis of starch from 2% CO2-grown cells was 1.25 times greater than that from air-grown cells and 2-11 times higher than the rates of hydrolysis of starches from cereal grains.
24474069	4	43	from	algae	557:561	arg1	granules					543:550	Starch granules	536:550	Starch granules from algae grown in ambient air and 2% CO2	536:593	Starch granules from algae grown in ambient air and 2% CO2 were analyzed by transmission electron microscopy (TEM) and scanning electron microscopy (SEM).
24474069	4	44	theme	Starch	536:541	arg1	granules					543:550	Starch granules	536:550	Starch granules from algae grown in ambient air and 2% CO2	536:593	Starch granules from algae grown in ambient air and 2% CO2 were analyzed by transmission electron microscopy (TEM) and scanning electron microscopy (SEM).
24474069	9	45	theme	hydrolysis	1535:1544	arg1	efficiency					1510:1519	the hydrolysis efficiency	1495:1519	the hydrolysis efficiency of the starch hydrolysis	1495:1544	These modifications in culture conditions increase the hydrolysis efficiency of the starch hydrolysis, thus providing potentially important gains for biofuel production.
24474069	4	46	theme	%	589:589	arg1	CO2					591:593	2% CO2	588:593	2% CO2	588:593	Starch granules from algae grown in ambient air and 2% CO2 were analyzed by transmission electron microscopy (TEM) and scanning electron microscopy (SEM).
24474069	3	47	theme	2	528:528	arg1	%					529:529	%	529:529	%	529:529	The production of C. sorokiniana biomass, lipid and starch were enhanced when cultures were supplied with 2% CO2.
24474069	1	48	theme	lipids	163:168	arg1	producers					150:158	important producers	140:158	important producers	140:158	Cultured microalgae are viewed as important producers of lipids and polysaccharides, both of which are precursor molecules for the production of biofuels.
24474069	1	48	theme	lipids	163:168	arg1	microalgae					115:124	Cultured microalgae	106:124	Cultured microalgae	106:124	Cultured microalgae are viewed as important producers of lipids and polysaccharides, both of which are precursor molecules for the production of biofuels.
24474069	5	49	with	cup-shaped	834:843	arg1	starch					869:874	primarily pyrenoid starch	850:874	primarily pyrenoid starch	850:874	The granules from algae grown in 2% CO2 were disk-shaped and contained mainly stromal starch; granules from cultures grown in ambient air were cup-shaped with primarily pyrenoid starch.
24474069	1	50	theme	polysaccharides	174:188	arg1	producers					150:158	important producers	140:158	important producers	140:158	Cultured microalgae are viewed as important producers of lipids and polysaccharides, both of which are precursor molecules for the production of biofuels.
24474069	1	50	theme	polysaccharides	174:188	arg1	microalgae					115:124	Cultured microalgae	106:124	Cultured microalgae	106:124	Cultured microalgae are viewed as important producers of lipids and polysaccharides, both of which are precursor molecules for the production of biofuels.
24474069	7	51	from	rate	1057:1060	arg1	cells					1104:1108	2% CO2-grown cells	1091:1108	2% CO2-grown cells	1091:1108	The rate of hydrolysis of starch from 2% CO2-grown cells was 1.25 times greater than that from air-grown cells and 2-11 times higher than the rates of hydrolysis of starches from cereal grains.
24474069	4	52	theme	ambient	572:578	arg1	air					580:582	ambient air	572:582	ambient air	572:582	Starch granules from algae grown in ambient air and 2% CO2 were analyzed by transmission electron microscopy (TEM) and scanning electron microscopy (SEM).
24474069	7	53	theme	cereal	1232:1237	arg1	grains					1239:1244	cereal grains	1232:1244	cereal grains	1232:1244	The rate of hydrolysis of starch from 2% CO2-grown cells was 1.25 times greater than that from air-grown cells and 2-11 times higher than the rates of hydrolysis of starches from cereal grains.
24474069	9	54	theme	biofuel	1594:1600	arg1	production					1602:1611	biofuel production	1594:1611	biofuel production	1594:1611	These modifications in culture conditions increase the hydrolysis efficiency of the starch hydrolysis, thus providing potentially important gains for biofuel production.
24474069	7	55	theme	starches	1218:1225	arg1	hydrolysis					1204:1213	hydrolysis	1204:1213	hydrolysis of starches from cereal grains	1204:1244	The rate of hydrolysis of starch from 2% CO2-grown cells was 1.25 times greater than that from air-grown cells and 2-11 times higher than the rates of hydrolysis of starches from cereal grains.
24474069	1	56	theme	Cultured	106:113	arg1	producers					150:158	important producers	140:158	important producers	140:158	Cultured microalgae are viewed as important producers of lipids and polysaccharides, both of which are precursor molecules for the production of biofuels.
24474069	1	56	theme	Cultured	106:113	arg1	microalgae					115:124	Cultured microalgae	106:124	Cultured microalgae	106:124	Cultured microalgae are viewed as important producers of lipids and polysaccharides, both of which are precursor molecules for the production of biofuels.
24474069	8	57	theme	biomass	1332:1338	arg1	yield					1340:1344	biomass yield	1332:1344	biomass yield	1332:1344	These data indicate that culturing C. sorokiniana in elevated CO2 not only increases biomass yield but also improves the structure and composition of starch granules for use in biofuel generation.
24474069	7	58	theme	hydrolysis	1204:1213	arg1	rates					1195:1199	the rates	1191:1199	the rates of hydrolysis of starches from cereal grains	1191:1244	The rate of hydrolysis of starch from 2% CO2-grown cells was 1.25 times greater than that from air-grown cells and 2-11 times higher than the rates of hydrolysis of starches from cereal grains.
24474069	2	59	theme	carbon	305:310	arg1	dioxide					312:318	elevated carbon dioxide	296:318	elevated carbon dioxide (CO2)	296:324	This study addressed the impact of elevated carbon dioxide (CO2) on Chlorella sorokiniana production of starch and on several properties of the starch produced.
24474069	2	59	theme	carbon	305:310	arg1	CO2					321:323	CO2	321:323	CO2	321:323	This study addressed the impact of elevated carbon dioxide (CO2) on Chlorella sorokiniana production of starch and on several properties of the starch produced.
24474069	7	60	theme	2	1091:1091	arg1	%					1092:1092	%	1092:1092	%	1092:1092	The rate of hydrolysis of starch from 2% CO2-grown cells was 1.25 times greater than that from air-grown cells and 2-11 times higher than the rates of hydrolysis of starches from cereal grains.
24474069	6	61	theme	%	911:911	arg1	CO2					913:915	2% CO2	910:915	2% CO2	910:915	The granules from cells grown in 2% CO2 had a higher proportion of the accumulated starch as the highly branched, amylopectin glucan than did granules from cells grown in air.
24474069	0	62	theme	starch	65:70	arg1	quality					48:54	the quality	44:54	the quality of algal starch	44:70	The impact of elevated CO2 concentration on the quality of algal starch as a potential biofuel feedstock.
24474069	0	63	from	impact	4:9	arg1	quality					48:54	the quality	44:54	the quality of algal starch	44:70	The impact of elevated CO2 concentration on the quality of algal starch as a potential biofuel feedstock.
24474069	4	64	theme	electron	625:632	arg1	TEM					646:648	TEM	646:648	TEM	646:648	Starch granules from algae grown in ambient air and 2% CO2 were analyzed by transmission electron microscopy (TEM) and scanning electron microscopy (SEM).
24474069	4	64	theme	electron	625:632	arg1	microscopy					634:643	transmission electron microscopy	612:643	transmission electron microscopy (TEM)	612:649	Starch granules from algae grown in ambient air and 2% CO2 were analyzed by transmission electron microscopy (TEM) and scanning electron microscopy (SEM).
24474069	7	65	theme	hydrolysis	1065:1074	arg1	rate					1057:1060	The rate	1053:1060	The rate of hydrolysis of starch from 2% CO2-grown cells	1053:1108	The rate of hydrolysis of starch from 2% CO2-grown cells was 1.25 times greater than that from air-grown cells and 2-11 times higher than the rates of hydrolysis of starches from cereal grains.
24474069	7	65	theme	hydrolysis	1065:1074	arg1	times					1119:1123	times	1119:1123	times	1119:1123	The rate of hydrolysis of starch from 2% CO2-grown cells was 1.25 times greater than that from air-grown cells and 2-11 times higher than the rates of hydrolysis of starches from cereal grains.
24474069	7	66	dep	times	1119:1123	arg1	greater					1125:1131	greater	1125:1131	greater	1125:1131	The rate of hydrolysis of starch from 2% CO2-grown cells was 1.25 times greater than that from air-grown cells and 2-11 times higher than the rates of hydrolysis of starches from cereal grains.
24474069	2	67	from	impact	286:291	arg1	production					351:360	Chlorella sorokiniana production	329:360	Chlorella sorokiniana production of starch	329:370	This study addressed the impact of elevated carbon dioxide (CO2) on Chlorella sorokiniana production of starch and on several properties of the starch produced.
24474069	2	67	from	impact	286:291	arg1	properties					387:396	several properties	379:396	several properties of the starch produced	379:419	This study addressed the impact of elevated carbon dioxide (CO2) on Chlorella sorokiniana production of starch and on several properties of the starch produced.
24474069	2	68	theme	elevated	296:303	arg1	dioxide					312:318	elevated carbon dioxide	296:318	elevated carbon dioxide (CO2)	296:324	This study addressed the impact of elevated carbon dioxide (CO2) on Chlorella sorokiniana production of starch and on several properties of the starch produced.
24474069	2	68	theme	elevated	296:303	arg1	CO2					321:323	CO2	321:323	CO2	321:323	This study addressed the impact of elevated carbon dioxide (CO2) on Chlorella sorokiniana production of starch and on several properties of the starch produced.
24474069	0	69	theme	biofuel	87:93	arg1	feedstock					95:103	a potential biofuel feedstock	75:103	a potential biofuel feedstock	75:103	The impact of elevated CO2 concentration on the quality of algal starch as a potential biofuel feedstock.
24474069	6	70	contain	had	917:919	arg2	glucan					1003:1008	the highly branched, amylopectin glucan	970:1008	the highly branched, amylopectin glucan than did granules from cells grown in air	970:1050	The granules from cells grown in 2% CO2 had a higher proportion of the accumulated starch as the highly branched, amylopectin glucan than did granules from cells grown in air.
24474069	6	70	contain	had	917:919	arg2	proportion					930:939	a higher proportion	921:939	a higher proportion of the accumulated starch	921:965	The granules from cells grown in 2% CO2 had a higher proportion of the accumulated starch as the highly branched, amylopectin glucan than did granules from cells grown in air.
24474069	6	70	contain	had	917:919	arg1	granules					881:888	The granules	877:888	The granules from cells grown in 2% CO2	877:915	The granules from cells grown in 2% CO2 had a higher proportion of the accumulated starch as the highly branched, amylopectin glucan than did granules from cells grown in air.
24474069	7	71	from	hydrolysis	1204:1213	arg1	grains					1239:1244	cereal grains	1232:1244	cereal grains	1232:1244	The rate of hydrolysis of starch from 2% CO2-grown cells was 1.25 times greater than that from air-grown cells and 2-11 times higher than the rates of hydrolysis of starches from cereal grains.
24474069	6	72	theme	accumulated	948:958	arg1	starch					960:965	the accumulated starch	944:965	the accumulated starch	944:965	The granules from cells grown in 2% CO2 had a higher proportion of the accumulated starch as the highly branched, amylopectin glucan than did granules from cells grown in air.
24474069	2	73	theme	starch	405:410	arg1	properties					387:396	several properties	379:396	several properties of the starch produced	379:419	This study addressed the impact of elevated carbon dioxide (CO2) on Chlorella sorokiniana production of starch and on several properties of the starch produced.
24474069	8	74	theme	starch	1397:1402	arg1	granules					1404:1411	starch granules	1397:1411	starch granules	1397:1411	These data indicate that culturing C. sorokiniana in elevated CO2 not only increases biomass yield but also improves the structure and composition of starch granules for use in biofuel generation.
24474069	4	75	theme	scanning	655:662	arg1	microscopy					673:682	scanning electron microscopy	655:682	scanning electron microscopy (SEM)	655:688	Starch granules from algae grown in ambient air and 2% CO2 were analyzed by transmission electron microscopy (TEM) and scanning electron microscopy (SEM).
24474069	4	75	theme	scanning	655:662	arg1	SEM					685:687	SEM	685:687	SEM	685:687	Starch granules from algae grown in ambient air and 2% CO2 were analyzed by transmission electron microscopy (TEM) and scanning electron microscopy (SEM).
24474069	2	76	theme	sorokiniana	339:349	arg1	production					351:360	Chlorella sorokiniana production	329:360	Chlorella sorokiniana production of starch	329:370	This study addressed the impact of elevated carbon dioxide (CO2) on Chlorella sorokiniana production of starch and on several properties of the starch produced.
24474069	0	77	theme	elevated	14:21	arg1	concentration					27:39	elevated CO2 concentration	14:39	elevated CO2 concentration	14:39	The impact of elevated CO2 concentration on the quality of algal starch as a potential biofuel feedstock.
24474069	2	78	theme	several	379:385	arg1	properties					387:396	several properties	379:396	several properties of the starch produced	379:419	This study addressed the impact of elevated carbon dioxide (CO2) on Chlorella sorokiniana production of starch and on several properties of the starch produced.
24474069	0	79	theme	concentration	27:39	arg1	impact					4:9	The impact	0:9	The impact of elevated CO2 concentration on the quality of algal starch	0:70	The impact of elevated CO2 concentration on the quality of algal starch as a potential biofuel feedstock.
24474069	6	80	theme	higher	923:928	arg1	proportion					930:939	a higher proportion	921:939	a higher proportion of the accumulated starch	921:965	The granules from cells grown in 2% CO2 had a higher proportion of the accumulated starch as the highly branched, amylopectin glucan than did granules from cells grown in air.
24474069	6	80	theme	higher	923:928	arg1	glucan					1003:1008	the highly branched, amylopectin glucan	970:1008	the highly branched, amylopectin glucan than did granules from cells grown in air	970:1050	The granules from cells grown in 2% CO2 had a higher proportion of the accumulated starch as the highly branched, amylopectin glucan than did granules from cells grown in air.
24474069	1	81	theme	precursor	209:217	arg1	producers					150:158	important producers	140:158	important producers	140:158	Cultured microalgae are viewed as important producers of lipids and polysaccharides, both of which are precursor molecules for the production of biofuels.
24474069	1	81	theme	precursor	209:217	arg1	molecules					219:227	precursor molecules	209:227	precursor molecules for the production of biofuels	209:258	Cultured microalgae are viewed as important producers of lipids and polysaccharides, both of which are precursor molecules for the production of biofuels.
24474069	1	81	theme	precursor	209:217	arg1	both					191:194	both	191:194	both	191:194	Cultured microalgae are viewed as important producers of lipids and polysaccharides, both of which are precursor molecules for the production of biofuels.
24474069	6	82	dep	branched	981:988	arg1	amylopectin					991:1001	amylopectin	991:1001	amylopectin	991:1001	The granules from cells grown in 2% CO2 had a higher proportion of the accumulated starch as the highly branched, amylopectin glucan than did granules from cells grown in air.
24474069	2	83	theme	dioxide	312:318	arg1	impact					286:291	the impact	282:291	the impact of elevated carbon dioxide (CO2) on Chlorella sorokiniana production of starch and on several properties of the starch produced	282:419	This study addressed the impact of elevated carbon dioxide (CO2) on Chlorella sorokiniana production of starch and on several properties of the starch produced.
24474069	9	84	theme	culture	1467:1473	arg1	conditions					1475:1484	culture conditions	1467:1484	culture conditions	1467:1484	These modifications in culture conditions increase the hydrolysis efficiency of the starch hydrolysis, thus providing potentially important gains for biofuel production.
24474069	3	85	theme	sorokiniana	443:453	arg1	production					426:435	The production	422:435	The production of C. sorokiniana biomass, lipid and starch	422:479	The production of C. sorokiniana biomass, lipid and starch were enhanced when cultures were supplied with 2% CO2.
24474069	0	86	dep	impact	4:9	arg1	feedstock					95:103	a potential biofuel feedstock	75:103	a potential biofuel feedstock	75:103	The impact of elevated CO2 concentration on the quality of algal starch as a potential biofuel feedstock.
28935541	8	0	theme	hydrogel/micelle	1374:1389	arg1	sustained-release					1462:1478	a promising sustained-release or site-specific drug delivery system	1450:1516	sustained-release	1462:1478	These results demonstrated that the pH-sensitive hydrogel/micelle composites constructed with biocompatible materials can be a promising sustained-release or site-specific drug delivery system for instable or hydrophobic drugs.
28935541	8	0	theme	hydrogel/micelle	1374:1389	arg1	composites					1391:1400	the pH-sensitive hydrogel/micelle composites	1357:1400	the pH-sensitive hydrogel/micelle composites constructed with biocompatible materials	1357:1441	These results demonstrated that the pH-sensitive hydrogel/micelle composites constructed with biocompatible materials can be a promising sustained-release or site-specific drug delivery system for instable or hydrophobic drugs.
28935541	5	1	from	characteristics	799:813	arg1	fluids					838:843	simulated digestive fluids	818:843	simulated digestive fluids	818:843	The physical characteristics in simulated digestive fluids were investigated, demonstrating that the ratio of hydrogel to micelle distinctly affected swelling, degradation and in vitro drug release behaviors.
28935541	1	2	theme	unimolecular	143:154	arg1	micelle					156:162	a novel cross-linked unimolecular micelle	122:162	a novel cross-linked unimolecular micelle based on chitosan	122:180	In this study, we present a novel cross-linked unimolecular micelle based on chitosan.
28935541	4	3	theme	polyelectrolyte	759:773	arg1	complexes					775:783	polyelectrolyte complexes	759:783	polyelectrolyte complexes	759:783	The morphological analysis showed that the micelles exhibited a smaller diameter of about 80nm in aqueous solution, but dilated to 100-200nm in hydrogel owing to the formation of polyelectrolyte complexes.
28935541	0	4	theme	micelle	76:82	arg1	composites					84:93	alginate hydrogel/chitosan micelle composites	49:93	alginate hydrogel/chitosan micelle composites	49:93	A novel controlled drug delivery system based on alginate hydrogel/chitosan micelle composites.
28935541	7	5	theme	release	1146:1152	arg1	mechanisms					1154:1163	Their corresponding release mechanisms	1126:1163	Their corresponding release mechanisms	1126:1163	Their corresponding release mechanisms revealed that the release of drug from these two formulations followed a complex process, in which several mechanisms were involved or occurred simultaneously.
28935541	2	6	theme	pH-sensitive	339:350	arg1	composites					369:378	the pH-sensitive hydrogel/micelle composites	335:378	the pH-sensitive hydrogel/micelle composites	335:378	For controlling drug delivery via oral administration, emodin (EMO) encapsulated micelles were loaded into sodium alginate hydrogel matrix to construct the pH-sensitive hydrogel/micelle composites.
28935541	4	7	from	solution	686:693	arg1	diameter					652:659	a smaller diameter	642:659	a smaller diameter of about 80nm in aqueous solution	642:693	The morphological analysis showed that the micelles exhibited a smaller diameter of about 80nm in aqueous solution, but dilated to 100-200nm in hydrogel owing to the formation of polyelectrolyte complexes.
28935541	8	8	theme	hydrophobic	1534:1544	arg1	drugs					1546:1550	instable or hydrophobic drugs	1522:1550	instable or hydrophobic drugs	1522:1550	These results demonstrated that the pH-sensitive hydrogel/micelle composites constructed with biocompatible materials can be a promising sustained-release or site-specific drug delivery system for instable or hydrophobic drugs.
28935541	1	9	attach	present	114:120	arg2	we					111:112	we	111:112	we	111:112	In this study, we present a novel cross-linked unimolecular micelle based on chitosan.
28935541	1	9	attach	present	114:120	arg1	study					104:108	this study	99:108	this study	99:108	In this study, we present a novel cross-linked unimolecular micelle based on chitosan.
28935541	7	10	theme	corresponding	1132:1144	arg1	mechanisms					1154:1163	Their corresponding release mechanisms	1126:1163	Their corresponding release mechanisms	1126:1163	Their corresponding release mechanisms revealed that the release of drug from these two formulations followed a complex process, in which several mechanisms were involved or occurred simultaneously.
28935541	8	11	theme	drug	1497:1500	arg1	system					1511:1516	a promising sustained-release or site-specific drug delivery system	1450:1516	system	1511:1516	These results demonstrated that the pH-sensitive hydrogel/micelle composites constructed with biocompatible materials can be a promising sustained-release or site-specific drug delivery system for instable or hydrophobic drugs.
28935541	3	12	theme	experimental	526:537	arg1	design					539:544	Box-Behnken experimental design	514:544	Box-Behnken experimental design	514:544	The optimized formulation of micelle that consists of 8.06% CaCl2, 1.71% chitosan and 26.52% β-GP was obtained by the combination of Box-Behnken experimental design and response surface methodology.
28935541	5	13	theme	drug	971:974	arg1	release					976:982	in vitro drug release	962:982	in vitro drug release	962:982	The physical characteristics in simulated digestive fluids were investigated, demonstrating that the ratio of hydrogel to micelle distinctly affected swelling, degradation and in vitro drug release behaviors.
28935541	3	14	theme	surface	559:565	arg1	methodology					567:577	response surface methodology	550:577	response surface methodology	550:577	The optimized formulation of micelle that consists of 8.06% CaCl2, 1.71% chitosan and 26.52% β-GP was obtained by the combination of Box-Behnken experimental design and response surface methodology.
28935541	3	15	theme	design	539:544	arg1	combination					499:509	the combination	495:509	the combination of Box-Behnken experimental design and response surface methodology	495:577	The optimized formulation of micelle that consists of 8.06% CaCl2, 1.71% chitosan and 26.52% β-GP was obtained by the combination of Box-Behnken experimental design and response surface methodology.
28935541	2	16	theme	hydrogel/micelle	352:367	arg1	composites					369:378	the pH-sensitive hydrogel/micelle composites	335:378	the pH-sensitive hydrogel/micelle composites	335:378	For controlling drug delivery via oral administration, emodin (EMO) encapsulated micelles were loaded into sodium alginate hydrogel matrix to construct the pH-sensitive hydrogel/micelle composites.
28935541	3	17	theme	methodology	567:577	arg1	combination					499:509	the combination	495:509	the combination of Box-Behnken experimental design and response surface methodology	495:577	The optimized formulation of micelle that consists of 8.06% CaCl2, 1.71% chitosan and 26.52% β-GP was obtained by the combination of Box-Behnken experimental design and response surface methodology.
28935541	4	18	theme	complexes	775:783	arg1	formation					746:754	the formation	742:754	the formation of polyelectrolyte complexes	742:783	The morphological analysis showed that the micelles exhibited a smaller diameter of about 80nm in aqueous solution, but dilated to 100-200nm in hydrogel owing to the formation of polyelectrolyte complexes.
28935541	4	19	from	80nm	670:673	arg1	solution					686:693	aqueous solution	678:693	aqueous solution	678:693	The morphological analysis showed that the micelles exhibited a smaller diameter of about 80nm in aqueous solution, but dilated to 100-200nm in hydrogel owing to the formation of polyelectrolyte complexes.
28935541	4	20	theme	morphological	584:596	arg1	analysis					598:605	The morphological analysis	580:605	The morphological analysis	580:605	The morphological analysis showed that the micelles exhibited a smaller diameter of about 80nm in aqueous solution, but dilated to 100-200nm in hydrogel owing to the formation of polyelectrolyte complexes.
28935541	5	21	dep	swelling	936:943	arg1	behaviors					984:992	behaviors	984:992	behaviors	984:992	The physical characteristics in simulated digestive fluids were investigated, demonstrating that the ratio of hydrogel to micelle distinctly affected swelling, degradation and in vitro drug release behaviors.
28935541	2	22	theme	encapsulated	251:262	arg1	micelles					264:271	emodin (EMO) encapsulated micelles	238:271	emodin (EMO) encapsulated micelles	238:271	For controlling drug delivery via oral administration, emodin (EMO) encapsulated micelles were loaded into sodium alginate hydrogel matrix to construct the pH-sensitive hydrogel/micelle composites.
28935541	6	23	theme	colon-specific	1102:1115	arg1	profile					1117:1123	a colon-specific profile	1100:1123	a colon-specific profile	1100:1123	The hydrogel/micelle (1:1) exhibited a sustained-release profile, while hydrogel/micelle (3:1) exhibited a colon-specific profile.
28935541	0	24	theme	novel	2:6	arg1	system					33:38	A novel controlled drug delivery system	0:38	A novel controlled drug delivery system	0:38	A novel controlled drug delivery system based on alginate hydrogel/chitosan micelle composites.
28935541	8	25	theme	delivery	1502:1509	arg1	system					1511:1516	a promising sustained-release or site-specific drug delivery system	1450:1516	system	1511:1516	These results demonstrated that the pH-sensitive hydrogel/micelle composites constructed with biocompatible materials can be a promising sustained-release or site-specific drug delivery system for instable or hydrophobic drugs.
28935541	2	26	theme	hydrogel	306:313	arg1	matrix					315:320	sodium alginate hydrogel matrix	290:320	sodium alginate hydrogel matrix	290:320	For controlling drug delivery via oral administration, emodin (EMO) encapsulated micelles were loaded into sodium alginate hydrogel matrix to construct the pH-sensitive hydrogel/micelle composites.
28935541	3	27	theme	8.06	435:438	arg1	%					439:439	%	439:439	%	439:439	The optimized formulation of micelle that consists of 8.06% CaCl2, 1.71% chitosan and 26.52% β-GP was obtained by the combination of Box-Behnken experimental design and response surface methodology.
28935541	5	28	theme	simulated	818:826	arg1	fluids					838:843	simulated digestive fluids	818:843	simulated digestive fluids	818:843	The physical characteristics in simulated digestive fluids were investigated, demonstrating that the ratio of hydrogel to micelle distinctly affected swelling, degradation and in vitro drug release behaviors.
28935541	2	29	theme	alginate	297:304	arg1	matrix					315:320	sodium alginate hydrogel matrix	290:320	sodium alginate hydrogel matrix	290:320	For controlling drug delivery via oral administration, emodin (EMO) encapsulated micelles were loaded into sodium alginate hydrogel matrix to construct the pH-sensitive hydrogel/micelle composites.
28935541	4	30	from	diameter	652:659	arg1	solution					686:693	aqueous solution	678:693	aqueous solution	678:693	The morphological analysis showed that the micelles exhibited a smaller diameter of about 80nm in aqueous solution, but dilated to 100-200nm in hydrogel owing to the formation of polyelectrolyte complexes.
28935541	3	31	theme	%	439:439	arg1	CaCl2					441:445	8.06% CaCl2	435:445	8.06% CaCl2	435:445	The optimized formulation of micelle that consists of 8.06% CaCl2, 1.71% chitosan and 26.52% β-GP was obtained by the combination of Box-Behnken experimental design and response surface methodology.
28935541	0	32	theme	drug	19:22	arg1	system					33:38	A novel controlled drug delivery system	0:38	A novel controlled drug delivery system	0:38	A novel controlled drug delivery system based on alginate hydrogel/chitosan micelle composites.
28935541	2	33	theme	sodium	290:295	arg1	matrix					315:320	sodium alginate hydrogel matrix	290:320	sodium alginate hydrogel matrix	290:320	For controlling drug delivery via oral administration, emodin (EMO) encapsulated micelles were loaded into sodium alginate hydrogel matrix to construct the pH-sensitive hydrogel/micelle composites.
28935541	0	34	theme	controlled	8:17	arg1	system					33:38	A novel controlled drug delivery system	0:38	A novel controlled drug delivery system	0:38	A novel controlled drug delivery system based on alginate hydrogel/chitosan micelle composites.
28935541	5	35	theme	in	962:963	arg1	release					976:982	in vitro drug release	962:982	in vitro drug release	962:982	The physical characteristics in simulated digestive fluids were investigated, demonstrating that the ratio of hydrogel to micelle distinctly affected swelling, degradation and in vitro drug release behaviors.
28935541	7	36	from	formulations	1214:1225	arg1	release					1183:1189	the release	1179:1189	the release of drug from these two formulations	1179:1225	Their corresponding release mechanisms revealed that the release of drug from these two formulations followed a complex process, in which several mechanisms were involved or occurred simultaneously.
28935541	3	37	theme	response	550:557	arg1	methodology					567:577	response surface methodology	550:577	response surface methodology	550:577	The optimized formulation of micelle that consists of 8.06% CaCl2, 1.71% chitosan and 26.52% β-GP was obtained by the combination of Box-Behnken experimental design and response surface methodology.
28935541	3	38	theme	optimized	385:393	arg1	formulation					395:405	The optimized formulation	381:405	The optimized formulation of micelle that consists of 8.06% CaCl2, 1.71% chitosan and 26.52% β-GP	381:477	The optimized formulation of micelle that consists of 8.06% CaCl2, 1.71% chitosan and 26.52% β-GP was obtained by the combination of Box-Behnken experimental design and response surface methodology.
28935541	8	39	theme	biocompatible	1419:1431	arg1	materials					1433:1441	biocompatible materials	1419:1441	biocompatible materials	1419:1441	These results demonstrated that the pH-sensitive hydrogel/micelle composites constructed with biocompatible materials can be a promising sustained-release or site-specific drug delivery system for instable or hydrophobic drugs.
28935541	8	40	theme	pH-sensitive	1361:1372	arg1	sustained-release					1462:1478	a promising sustained-release or site-specific drug delivery system	1450:1516	sustained-release	1462:1478	These results demonstrated that the pH-sensitive hydrogel/micelle composites constructed with biocompatible materials can be a promising sustained-release or site-specific drug delivery system for instable or hydrophobic drugs.
28935541	8	40	theme	pH-sensitive	1361:1372	arg1	composites					1391:1400	the pH-sensitive hydrogel/micelle composites	1357:1400	the pH-sensitive hydrogel/micelle composites constructed with biocompatible materials	1357:1441	These results demonstrated that the pH-sensitive hydrogel/micelle composites constructed with biocompatible materials can be a promising sustained-release or site-specific drug delivery system for instable or hydrophobic drugs.
28935541	4	41	theme	80nm	670:673	arg1	diameter					652:659	a smaller diameter	642:659	a smaller diameter of about 80nm in aqueous solution	642:693	The morphological analysis showed that the micelles exhibited a smaller diameter of about 80nm in aqueous solution, but dilated to 100-200nm in hydrogel owing to the formation of polyelectrolyte complexes.
28935541	3	42	theme	1.71	448:451	arg1	%					452:452	%	452:452	%	452:452	The optimized formulation of micelle that consists of 8.06% CaCl2, 1.71% chitosan and 26.52% β-GP was obtained by the combination of Box-Behnken experimental design and response surface methodology.
28935541	0	43	theme	delivery	24:31	arg1	system					33:38	A novel controlled drug delivery system	0:38	A novel controlled drug delivery system	0:38	A novel controlled drug delivery system based on alginate hydrogel/chitosan micelle composites.
28935541	1	44	link	cross-linked	130:141	arg1	micelle					156:162	a novel cross-linked unimolecular micelle	122:162	a novel cross-linked unimolecular micelle based on chitosan	122:180	In this study, we present a novel cross-linked unimolecular micelle based on chitosan.
28935541	5	45	theme	digestive	828:836	arg1	fluids					838:843	simulated digestive fluids	818:843	simulated digestive fluids	818:843	The physical characteristics in simulated digestive fluids were investigated, demonstrating that the ratio of hydrogel to micelle distinctly affected swelling, degradation and in vitro drug release behaviors.
28935541	5	46	theme	physical	790:797	arg1	characteristics					799:813	The physical characteristics	786:813	The physical characteristics in simulated digestive fluids	786:843	The physical characteristics in simulated digestive fluids were investigated, demonstrating that the ratio of hydrogel to micelle distinctly affected swelling, degradation and in vitro drug release behaviors.
28935541	3	47	theme	%	452:452	arg1	chitosan					454:461	1.71% chitosan	448:461	1.71% chitosan	448:461	The optimized formulation of micelle that consists of 8.06% CaCl2, 1.71% chitosan and 26.52% β-GP was obtained by the combination of Box-Behnken experimental design and response surface methodology.
28935541	3	48	theme	micelle	410:416	arg1	formulation					395:405	The optimized formulation	381:405	The optimized formulation of micelle that consists of 8.06% CaCl2, 1.71% chitosan and 26.52% β-GP	381:477	The optimized formulation of micelle that consists of 8.06% CaCl2, 1.71% chitosan and 26.52% β-GP was obtained by the combination of Box-Behnken experimental design and response surface methodology.
28935541	5	49	theme	hydrogel	896:903	arg1	ratio					887:891	the ratio	883:891	the ratio of hydrogel to micelle	883:914	The physical characteristics in simulated digestive fluids were investigated, demonstrating that the ratio of hydrogel to micelle distinctly affected swelling, degradation and in vitro drug release behaviors.
28935541	2	50	theme	oral	217:220	arg1	administration					222:235	oral administration	217:235	oral administration	217:235	For controlling drug delivery via oral administration, emodin (EMO) encapsulated micelles were loaded into sodium alginate hydrogel matrix to construct the pH-sensitive hydrogel/micelle composites.
28935541	5	51	dep	in	962:963	arg1	vitro					965:969	vitro	965:969	vitro	965:969	The physical characteristics in simulated digestive fluids were investigated, demonstrating that the ratio of hydrogel to micelle distinctly affected swelling, degradation and in vitro drug release behaviors.
28935541	8	52	theme	site-specific	1483:1495	arg1	system					1511:1516	a promising sustained-release or site-specific drug delivery system	1450:1516	system	1511:1516	These results demonstrated that the pH-sensitive hydrogel/micelle composites constructed with biocompatible materials can be a promising sustained-release or site-specific drug delivery system for instable or hydrophobic drugs.
28935541	7	53	theme	drug	1194:1197	arg1	release					1183:1189	the release	1179:1189	the release of drug from these two formulations	1179:1225	Their corresponding release mechanisms revealed that the release of drug from these two formulations followed a complex process, in which several mechanisms were involved or occurred simultaneously.
28935541	2	54	theme	drug	199:202	arg1	delivery					204:211	drug delivery	199:211	drug delivery	199:211	For controlling drug delivery via oral administration, emodin (EMO) encapsulated micelles were loaded into sodium alginate hydrogel matrix to construct the pH-sensitive hydrogel/micelle composites.
28935541	4	55	theme	aqueous	678:684	arg1	solution					686:693	aqueous solution	678:693	aqueous solution	678:693	The morphological analysis showed that the micelles exhibited a smaller diameter of about 80nm in aqueous solution, but dilated to 100-200nm in hydrogel owing to the formation of polyelectrolyte complexes.
28935541	3	56	theme	26.52	467:471	arg1	%					472:472	%	472:472	%	472:472	The optimized formulation of micelle that consists of 8.06% CaCl2, 1.71% chitosan and 26.52% β-GP was obtained by the combination of Box-Behnken experimental design and response surface methodology.
28935541	8	57	theme	promising	1452:1460	arg1	sustained-release					1462:1478	a promising sustained-release or site-specific drug delivery system	1450:1516	sustained-release	1462:1478	These results demonstrated that the pH-sensitive hydrogel/micelle composites constructed with biocompatible materials can be a promising sustained-release or site-specific drug delivery system for instable or hydrophobic drugs.
28935541	8	57	theme	promising	1452:1460	arg1	composites					1391:1400	the pH-sensitive hydrogel/micelle composites	1357:1400	the pH-sensitive hydrogel/micelle composites constructed with biocompatible materials	1357:1441	These results demonstrated that the pH-sensitive hydrogel/micelle composites constructed with biocompatible materials can be a promising sustained-release or site-specific drug delivery system for instable or hydrophobic drugs.
28935541	3	58	theme	Box-Behnken	514:524	arg1	design					539:544	Box-Behnken experimental design	514:544	Box-Behnken experimental design	514:544	The optimized formulation of micelle that consists of 8.06% CaCl2, 1.71% chitosan and 26.52% β-GP was obtained by the combination of Box-Behnken experimental design and response surface methodology.
28935541	2	59	theme	EMO	246:248	arg1	micelles					264:271	emodin (EMO) encapsulated micelles	238:271	emodin (EMO) encapsulated micelles	238:271	For controlling drug delivery via oral administration, emodin (EMO) encapsulated micelles were loaded into sodium alginate hydrogel matrix to construct the pH-sensitive hydrogel/micelle composites.
28935541	3	60	theme	%	472:472	arg1	β-GP					474:477	26.52% β-GP	467:477	26.52% β-GP	467:477	The optimized formulation of micelle that consists of 8.06% CaCl2, 1.71% chitosan and 26.52% β-GP was obtained by the combination of Box-Behnken experimental design and response surface methodology.
28935541	6	61	theme	sustained-release	1034:1050	arg1	profile					1052:1058	a sustained-release profile	1032:1058	a sustained-release profile	1032:1058	The hydrogel/micelle (1:1) exhibited a sustained-release profile, while hydrogel/micelle (3:1) exhibited a colon-specific profile.
28935541	0	62	theme	hydrogel/chitosan	58:74	arg1	composites					84:93	alginate hydrogel/chitosan micelle composites	49:93	alginate hydrogel/chitosan micelle composites	49:93	A novel controlled drug delivery system based on alginate hydrogel/chitosan micelle composites.
28935541	4	63	theme	smaller	644:650	arg1	diameter					652:659	a smaller diameter	642:659	a smaller diameter of about 80nm in aqueous solution	642:693	The morphological analysis showed that the micelles exhibited a smaller diameter of about 80nm in aqueous solution, but dilated to 100-200nm in hydrogel owing to the formation of polyelectrolyte complexes.
28935541	8	64	theme	instable	1522:1529	arg1	drugs					1546:1550	instable or hydrophobic drugs	1522:1550	instable or hydrophobic drugs	1522:1550	These results demonstrated that the pH-sensitive hydrogel/micelle composites constructed with biocompatible materials can be a promising sustained-release or site-specific drug delivery system for instable or hydrophobic drugs.
28935541	1	65	theme	novel	124:128	arg1	micelle					156:162	a novel cross-linked unimolecular micelle	122:162	a novel cross-linked unimolecular micelle based on chitosan	122:180	In this study, we present a novel cross-linked unimolecular micelle based on chitosan.
28935541	0	66	theme	alginate	49:56	arg1	composites					84:93	alginate hydrogel/chitosan micelle composites	49:93	alginate hydrogel/chitosan micelle composites	49:93	A novel controlled drug delivery system based on alginate hydrogel/chitosan micelle composites.
28935541	2	67	theme	emodin	238:243	arg1	micelles					264:271	emodin (EMO) encapsulated micelles	238:271	emodin (EMO) encapsulated micelles	238:271	For controlling drug delivery via oral administration, emodin (EMO) encapsulated micelles were loaded into sodium alginate hydrogel matrix to construct the pH-sensitive hydrogel/micelle composites.
28935541	1	68	theme	cross-linked	130:141	arg1	micelle					156:162	a novel cross-linked unimolecular micelle	122:162	a novel cross-linked unimolecular micelle based on chitosan	122:180	In this study, we present a novel cross-linked unimolecular micelle based on chitosan.
28935541	7	69	theme	several	1264:1270	arg1	mechanisms					1272:1281	several mechanisms	1264:1281	several mechanisms	1264:1281	Their corresponding release mechanisms revealed that the release of drug from these two formulations followed a complex process, in which several mechanisms were involved or occurred simultaneously.
28935541	7	70	theme	complex	1238:1244	arg1	process					1246:1252	a complex process	1236:1252	a complex process	1236:1252	Their corresponding release mechanisms revealed that the release of drug from these two formulations followed a complex process, in which several mechanisms were involved or occurred simultaneously.
25701776	3	0	theme	first	350:354	arg1	time					356:359	the first time	346:359	the first time	346:359	For the first time, in this work we prepared monolithic silica-chitin composite under extreme biomimetic conditions (80°C and pH 1.5) using three dimensional chitinous matrices isolated from the marine sponge Aplysina cauliformis.
25701776	1	1	theme	natural	170:176	arg1	world					178:182	the natural world	166:182	the natural world	166:182	Chitin is a widespread renewable biopolymer that is extensively distributed in the natural world.
25701776	0	2	theme	monolithic	15:24	arg1	composite					40:48	monolithic silica-chitin composite	15:48	monolithic silica-chitin composite	15:48	Preparation of monolithic silica-chitin composite under extreme biomimetic conditions.
25701776	3	3	theme	marine	537:542	arg1	cauliformis					560:570	the marine sponge Aplysina cauliformis	533:570	the marine sponge Aplysina cauliformis	533:570	For the first time, in this work we prepared monolithic silica-chitin composite under extreme biomimetic conditions (80°C and pH 1.5) using three dimensional chitinous matrices isolated from the marine sponge Aplysina cauliformis.
25701776	5	4	theme	hydrothermal	790:801	arg1	conditions					803:812	these hydrothermal conditions	784:812	these hydrothermal conditions	784:812	A mechanism for the silica-chitin interaction after exposure to these hydrothermal conditions is proposed and discussed.
25701776	4	5	theme	fluorescence	624:635	arg1	microscopy					637:646	fluorescence microscopy	624:646	fluorescence microscopy	624:646	The resulting material was studied using light and fluorescence microscopy, scanning electron microscopy, Fourier transform infrared spectroscopy.
25701776	2	6	theme	synthesis	311:319	arg1	conditions					321:330	hydrothermal synthesis conditions	298:330	hydrothermal synthesis conditions	298:330	The high thermal stability of chitin provides an opportunity to develop novel inorganic-organic composites under hydrothermal synthesis conditions in vitro.
25701776	3	7	theme	biomimetic	436:445	arg1	pH					468:469	pH 1.5	468:473	pH 1.5	468:473	For the first time, in this work we prepared monolithic silica-chitin composite under extreme biomimetic conditions (80°C and pH 1.5) using three dimensional chitinous matrices isolated from the marine sponge Aplysina cauliformis.
25701776	3	7	theme	biomimetic	436:445	arg1	80°C					459:462	80°C	459:462	80°C	459:462	For the first time, in this work we prepared monolithic silica-chitin composite under extreme biomimetic conditions (80°C and pH 1.5) using three dimensional chitinous matrices isolated from the marine sponge Aplysina cauliformis.
25701776	3	7	theme	biomimetic	436:445	arg1	conditions					447:456	extreme biomimetic conditions	428:456	extreme biomimetic conditions (80°C and pH 1.5)	428:474	For the first time, in this work we prepared monolithic silica-chitin composite under extreme biomimetic conditions (80°C and pH 1.5) using three dimensional chitinous matrices isolated from the marine sponge Aplysina cauliformis.
25701776	2	8	theme	hydrothermal	298:309	arg1	conditions					321:330	hydrothermal synthesis conditions	298:330	hydrothermal synthesis conditions	298:330	The high thermal stability of chitin provides an opportunity to develop novel inorganic-organic composites under hydrothermal synthesis conditions in vitro.
25701776	3	9	dep	conditions	447:456	arg1	pH					468:469	pH 1.5	468:473	pH 1.5	468:473	For the first time, in this work we prepared monolithic silica-chitin composite under extreme biomimetic conditions (80°C and pH 1.5) using three dimensional chitinous matrices isolated from the marine sponge Aplysina cauliformis.
25701776	3	9	dep	conditions	447:456	arg1	80°C					459:462	80°C	459:462	80°C	459:462	For the first time, in this work we prepared monolithic silica-chitin composite under extreme biomimetic conditions (80°C and pH 1.5) using three dimensional chitinous matrices isolated from the marine sponge Aplysina cauliformis.
25701776	3	9	dep	conditions	447:456	arg1	conditions					447:456	extreme biomimetic conditions	428:456	extreme biomimetic conditions (80°C and pH 1.5)	428:474	For the first time, in this work we prepared monolithic silica-chitin composite under extreme biomimetic conditions (80°C and pH 1.5) using three dimensional chitinous matrices isolated from the marine sponge Aplysina cauliformis.
25701776	1	10	theme	widespread	99:108	arg1	Chitin					87:92	Chitin	87:92	Chitin	87:92	Chitin is a widespread renewable biopolymer that is extensively distributed in the natural world.
25701776	1	10	theme	widespread	99:108	arg1	biopolymer					120:129	a widespread renewable biopolymer	97:129	a widespread renewable biopolymer that is extensively distributed in the natural world	97:182	Chitin is a widespread renewable biopolymer that is extensively distributed in the natural world.
25701776	3	11	theme	monolithic	387:396	arg1	composite					412:420	monolithic silica-chitin composite	387:420	monolithic silica-chitin composite	387:420	For the first time, in this work we prepared monolithic silica-chitin composite under extreme biomimetic conditions (80°C and pH 1.5) using three dimensional chitinous matrices isolated from the marine sponge Aplysina cauliformis.
25701776	1	12	theme	renewable	110:118	arg1	Chitin					87:92	Chitin	87:92	Chitin	87:92	Chitin is a widespread renewable biopolymer that is extensively distributed in the natural world.
25701776	1	12	theme	renewable	110:118	arg1	biopolymer					120:129	a widespread renewable biopolymer	97:129	a widespread renewable biopolymer that is extensively distributed in the natural world	97:182	Chitin is a widespread renewable biopolymer that is extensively distributed in the natural world.
25701776	3	13	theme	silica-chitin	398:410	arg1	composite					412:420	monolithic silica-chitin composite	387:420	monolithic silica-chitin composite	387:420	For the first time, in this work we prepared monolithic silica-chitin composite under extreme biomimetic conditions (80°C and pH 1.5) using three dimensional chitinous matrices isolated from the marine sponge Aplysina cauliformis.
25701776	2	14	theme	thermal	194:200	arg1	stability					202:210	The high thermal stability	185:210	The high thermal stability of chitin	185:220	The high thermal stability of chitin provides an opportunity to develop novel inorganic-organic composites under hydrothermal synthesis conditions in vitro.
25701776	3	15	theme	dimensional	488:498	arg1	matrices					510:517	three dimensional chitinous matrices	482:517	three dimensional chitinous matrices isolated from the marine sponge Aplysina cauliformis	482:570	For the first time, in this work we prepared monolithic silica-chitin composite under extreme biomimetic conditions (80°C and pH 1.5) using three dimensional chitinous matrices isolated from the marine sponge Aplysina cauliformis.
25701776	2	16	theme	high	189:192	arg1	stability					202:210	The high thermal stability	185:210	The high thermal stability of chitin	185:220	The high thermal stability of chitin provides an opportunity to develop novel inorganic-organic composites under hydrothermal synthesis conditions in vitro.
25701776	0	17	theme	composite	40:48	arg1	Preparation					0:10	Preparation	0:10	Preparation of monolithic silica-chitin composite under extreme biomimetic conditions.	0:85	Preparation of monolithic silica-chitin composite under extreme biomimetic conditions.
25701776	0	18	theme	silica-chitin	26:38	arg1	composite					40:48	monolithic silica-chitin composite	15:48	monolithic silica-chitin composite	15:48	Preparation of monolithic silica-chitin composite under extreme biomimetic conditions.
25701776	0	19	theme	biomimetic	64:73	arg1	conditions					75:84	extreme biomimetic conditions	56:84	extreme biomimetic conditions	56:84	Preparation of monolithic silica-chitin composite under extreme biomimetic conditions.
25701776	4	20	dep	Fourier	679:685	arg1	transform					687:695	transform	687:695	transform infrared spectroscopy	687:717	The resulting material was studied using light and fluorescence microscopy, scanning electron microscopy, Fourier transform infrared spectroscopy.
25701776	0	21	theme	extreme	56:62	arg1	conditions					75:84	extreme biomimetic conditions	56:84	extreme biomimetic conditions	56:84	Preparation of monolithic silica-chitin composite under extreme biomimetic conditions.
25701776	4	22	theme	resulting	577:585	arg1	material					587:594	The resulting material	573:594	The resulting material	573:594	The resulting material was studied using light and fluorescence microscopy, scanning electron microscopy, Fourier transform infrared spectroscopy.
25701776	5	23	theme	silica-chitin	740:752	arg1	interaction					754:764	the silica-chitin interaction	736:764	the silica-chitin interaction after exposure to these hydrothermal conditions	736:812	A mechanism for the silica-chitin interaction after exposure to these hydrothermal conditions is proposed and discussed.
25701776	2	24	theme	chitin	215:220	arg1	stability					202:210	The high thermal stability	185:210	The high thermal stability of chitin	185:220	The high thermal stability of chitin provides an opportunity to develop novel inorganic-organic composites under hydrothermal synthesis conditions in vitro.
25701776	4	25	dep	transform	687:695	arg1	infrared					697:704	infrared	697:704	transform infrared spectroscopy	687:717	The resulting material was studied using light and fluorescence microscopy, scanning electron microscopy, Fourier transform infrared spectroscopy.
25701776	3	26	theme	chitinous	500:508	arg1	matrices					510:517	three dimensional chitinous matrices	482:517	three dimensional chitinous matrices isolated from the marine sponge Aplysina cauliformis	482:570	For the first time, in this work we prepared monolithic silica-chitin composite under extreme biomimetic conditions (80°C and pH 1.5) using three dimensional chitinous matrices isolated from the marine sponge Aplysina cauliformis.
25701776	4	27	theme	scanning	649:656	arg1	light					614:618	light	614:618	light	614:618	The resulting material was studied using light and fluorescence microscopy, scanning electron microscopy, Fourier transform infrared spectroscopy.
25701776	4	27	theme	scanning	649:656	arg1	microscopy					667:676	scanning electron microscopy	649:676	scanning electron microscopy	649:676	The resulting material was studied using light and fluorescence microscopy, scanning electron microscopy, Fourier transform infrared spectroscopy.
25701776	2	28	theme	inorganic-organic	263:279	arg1	composites					281:290	novel inorganic-organic composites	257:290	novel inorganic-organic composites	257:290	The high thermal stability of chitin provides an opportunity to develop novel inorganic-organic composites under hydrothermal synthesis conditions in vitro.
25701776	3	29	attach	isolated	519:526	arg1	cauliformis					560:570	the marine sponge Aplysina cauliformis	533:570	the marine sponge Aplysina cauliformis	533:570	For the first time, in this work we prepared monolithic silica-chitin composite under extreme biomimetic conditions (80°C and pH 1.5) using three dimensional chitinous matrices isolated from the marine sponge Aplysina cauliformis.
25701776	3	29	attach	isolated	519:526	arg2	matrices					510:517	three dimensional chitinous matrices	482:517	three dimensional chitinous matrices isolated from the marine sponge Aplysina cauliformis	482:570	For the first time, in this work we prepared monolithic silica-chitin composite under extreme biomimetic conditions (80°C and pH 1.5) using three dimensional chitinous matrices isolated from the marine sponge Aplysina cauliformis.
25701776	3	30	theme	sponge	544:549	arg1	cauliformis					560:570	the marine sponge Aplysina cauliformis	533:570	the marine sponge Aplysina cauliformis	533:570	For the first time, in this work we prepared monolithic silica-chitin composite under extreme biomimetic conditions (80°C and pH 1.5) using three dimensional chitinous matrices isolated from the marine sponge Aplysina cauliformis.
25701776	3	31	theme	extreme	428:434	arg1	pH					468:469	pH 1.5	468:473	pH 1.5	468:473	For the first time, in this work we prepared monolithic silica-chitin composite under extreme biomimetic conditions (80°C and pH 1.5) using three dimensional chitinous matrices isolated from the marine sponge Aplysina cauliformis.
25701776	3	31	theme	extreme	428:434	arg1	80°C					459:462	80°C	459:462	80°C	459:462	For the first time, in this work we prepared monolithic silica-chitin composite under extreme biomimetic conditions (80°C and pH 1.5) using three dimensional chitinous matrices isolated from the marine sponge Aplysina cauliformis.
25701776	3	31	theme	extreme	428:434	arg1	conditions					447:456	extreme biomimetic conditions	428:456	extreme biomimetic conditions (80°C and pH 1.5)	428:474	For the first time, in this work we prepared monolithic silica-chitin composite under extreme biomimetic conditions (80°C and pH 1.5) using three dimensional chitinous matrices isolated from the marine sponge Aplysina cauliformis.
25701776	2	32	theme	novel	257:261	arg1	composites					281:290	novel inorganic-organic composites	257:290	novel inorganic-organic composites	257:290	The high thermal stability of chitin provides an opportunity to develop novel inorganic-organic composites under hydrothermal synthesis conditions in vitro.
25701776	3	33	theme	Aplysina	551:558	arg1	cauliformis					560:570	the marine sponge Aplysina cauliformis	533:570	the marine sponge Aplysina cauliformis	533:570	For the first time, in this work we prepared monolithic silica-chitin composite under extreme biomimetic conditions (80°C and pH 1.5) using three dimensional chitinous matrices isolated from the marine sponge Aplysina cauliformis.
25701776	4	34	theme	electron	658:665	arg1	light					614:618	light	614:618	light	614:618	The resulting material was studied using light and fluorescence microscopy, scanning electron microscopy, Fourier transform infrared spectroscopy.
25701776	4	34	theme	electron	658:665	arg1	microscopy					667:676	scanning electron microscopy	649:676	scanning electron microscopy	649:676	The resulting material was studied using light and fluorescence microscopy, scanning electron microscopy, Fourier transform infrared spectroscopy.
27958712	2	0	theme	Pickering	478:486	arg1	emulsion					488:495	a CNF-stabilized Pickering emulsion	461:495	a CNF-stabilized Pickering emulsion	461:495	Here, we report a facile aqueous preparation process for nanostructured polystyrene (PS)/CNF composites via the formation of a CNF-stabilized Pickering emulsion.
27958712	6	1	theme	materials	1024:1032	arg1	preparation					977:987	the preparation	973:987	the preparation of novel CNF/polymer nanocomposite materials	973:1032	Thus, this technique provides a simple and environmentally friendly method for the preparation of novel CNF/polymer nanocomposite materials.
27958712	5	2	theme	high	822:825	arg1	transparency					835:846	high optical transparency	822:846	high optical transparency	822:846	The PS/CNF nanocomposite showed high optical transparency, strength, and thermal dimensional stability.
27958712	3	3	theme	PS	498:499	arg1	nanoparticles					501:513	PS nanoparticles	498:513	PS nanoparticles	498:513	PS nanoparticles, with a narrow size distribution, were synthesized by free radical polymerization in water using CNF as a stabilizer.
27958712	6	4	theme	CNF/polymer	998:1008	arg1	materials					1024:1032	novel CNF/polymer nanocomposite materials	992:1032	novel CNF/polymer nanocomposite materials	992:1032	Thus, this technique provides a simple and environmentally friendly method for the preparation of novel CNF/polymer nanocomposite materials.
27958712	0	5	theme	Nanocellulose/Polymer	58:78	arg1	Nanocomposites					80:93	Transparent, Strong, and Thermally Stable Nanocellulose/Polymer Nanocomposites	16:93	Transparent, Strong, and Thermally Stable Nanocellulose/Polymer Nanocomposites from an Aqueous Pickering Emulsion	16:128	Facile Route to Transparent, Strong, and Thermally Stable Nanocellulose/Polymer Nanocomposites from an Aqueous Pickering Emulsion.
27958712	3	6	with	nanoparticles	501:513	arg1	distribution					535:546	a narrow size distribution	521:546	a narrow size distribution	521:546	PS nanoparticles, with a narrow size distribution, were synthesized by free radical polymerization in water using CNF as a stabilizer.
27958712	6	7	theme	novel	992:996	arg1	materials					1024:1032	novel CNF/polymer nanocomposite materials	992:1032	novel CNF/polymer nanocomposite materials	992:1032	Thus, this technique provides a simple and environmentally friendly method for the preparation of novel CNF/polymer nanocomposite materials.
27958712	4	8	from	embedded	761:768	arg1	framework					779:787	a CNF framework	773:787	a CNF framework	773:787	The nanoparticles were easily collected by filtration, and the resulting material had a composite structure of PS nanoparticles embedded in a CNF framework.
27958712	3	9	theme	size	530:533	arg1	distribution					535:546	a narrow size distribution	521:546	a narrow size distribution	521:546	PS nanoparticles, with a narrow size distribution, were synthesized by free radical polymerization in water using CNF as a stabilizer.
27958712	4	10	theme	PS	744:745	arg1	nanoparticles					747:759	PS nanoparticles	744:759	PS nanoparticles embedded in a CNF framework	744:787	The nanoparticles were easily collected by filtration, and the resulting material had a composite structure of PS nanoparticles embedded in a CNF framework.
27958712	0	11	theme	Pickering	111:119	arg1	Emulsion					121:128	an Aqueous Pickering Emulsion	100:128	an Aqueous Pickering Emulsion	100:128	Facile Route to Transparent, Strong, and Thermally Stable Nanocellulose/Polymer Nanocomposites from an Aqueous Pickering Emulsion.
27958712	4	12	contain	had	715:717	arg1	material					706:713	the resulting material	692:713	the resulting material	692:713	The nanoparticles were easily collected by filtration, and the resulting material had a composite structure of PS nanoparticles embedded in a CNF framework.
27958712	4	12	contain	had	715:717	arg2	structure					731:739	a composite structure	719:739	a composite structure of PS nanoparticles embedded in a CNF framework	719:787	The nanoparticles were easily collected by filtration, and the resulting material had a composite structure of PS nanoparticles embedded in a CNF framework.
27958712	5	13	theme	optical	827:833	arg1	transparency					835:846	high optical transparency	822:846	high optical transparency	822:846	The PS/CNF nanocomposite showed high optical transparency, strength, and thermal dimensional stability.
27958712	0	14	theme	Aqueous	103:109	arg1	Emulsion					121:128	an Aqueous Pickering Emulsion	100:128	an Aqueous Pickering Emulsion	100:128	Facile Route to Transparent, Strong, and Thermally Stable Nanocellulose/Polymer Nanocomposites from an Aqueous Pickering Emulsion.
27958712	3	15	theme	narrow	523:528	arg1	distribution					535:546	a narrow size distribution	521:546	a narrow size distribution	521:546	PS nanoparticles, with a narrow size distribution, were synthesized by free radical polymerization in water using CNF as a stabilizer.
27958712	1	16	theme	promising	163:171	arg1	nanofiller					173:182	a promising nanofiller	161:182	a promising nanofiller for polymer nanocomposite materials	161:218	Cellulose nanofibril (CNF) is a promising nanofiller for polymer nanocomposite materials, and a critical challenge in designing these materials is organization of the nanostructure using a facile process.
27958712	1	16	theme	promising	163:171	arg1	nanofibril					141:150	Cellulose nanofibril	131:150	Cellulose nanofibril (CNF)	131:156	Cellulose nanofibril (CNF) is a promising nanofiller for polymer nanocomposite materials, and a critical challenge in designing these materials is organization of the nanostructure using a facile process.
27958712	2	17	theme	CNF-stabilized	463:476	arg1	emulsion					488:495	a CNF-stabilized Pickering emulsion	461:495	a CNF-stabilized Pickering emulsion	461:495	Here, we report a facile aqueous preparation process for nanostructured polystyrene (PS)/CNF composites via the formation of a CNF-stabilized Pickering emulsion.
27958712	4	18	theme	composite	721:729	arg1	structure					731:739	a composite structure	719:739	a composite structure of PS nanoparticles embedded in a CNF framework	719:787	The nanoparticles were easily collected by filtration, and the resulting material had a composite structure of PS nanoparticles embedded in a CNF framework.
27958712	0	19	theme	Facile	0:5	arg1	Route					7:11	Facile Route	0:11	Facile Route to Transparent, Strong, and Thermally Stable Nanocellulose/Polymer Nanocomposites from an Aqueous Pickering Emulsion.	0:129	Facile Route to Transparent, Strong, and Thermally Stable Nanocellulose/Polymer Nanocomposites from an Aqueous Pickering Emulsion.
27958712	2	20	theme	aqueous	361:367	arg1	preparation					369:379	a facile aqueous preparation	352:379	a facile aqueous preparation process for nanostructured polystyrene (PS)/CNF composites via the formation of a CNF-stabilized Pickering emulsion	352:495	Here, we report a facile aqueous preparation process for nanostructured polystyrene (PS)/CNF composites via the formation of a CNF-stabilized Pickering emulsion.
27958712	0	21	theme	Transparent	16:26	arg1	Nanocomposites					80:93	Transparent, Strong, and Thermally Stable Nanocellulose/Polymer Nanocomposites	16:93	Transparent, Strong, and Thermally Stable Nanocellulose/Polymer Nanocomposites from an Aqueous Pickering Emulsion	16:128	Facile Route to Transparent, Strong, and Thermally Stable Nanocellulose/Polymer Nanocomposites from an Aqueous Pickering Emulsion.
27958712	5	22	theme	PS/CNF	794:799	arg1	nanocomposite					801:813	The PS/CNF nanocomposite	790:813	The PS/CNF nanocomposite	790:813	The PS/CNF nanocomposite showed high optical transparency, strength, and thermal dimensional stability.
27958712	2	23	theme	PS	421:422	arg1	composites					429:438	nanostructured polystyrene (PS)/CNF composites	393:438	nanostructured polystyrene (PS)/CNF composites	393:438	Here, we report a facile aqueous preparation process for nanostructured polystyrene (PS)/CNF composites via the formation of a CNF-stabilized Pickering emulsion.
27958712	4	24	from	framework	779:787	arg1	embedded					761:768	embedded	761:768	embedded	761:768	The nanoparticles were easily collected by filtration, and the resulting material had a composite structure of PS nanoparticles embedded in a CNF framework.
27958712	1	25	theme	nanocomposite	196:208	arg1	materials					210:218	polymer nanocomposite materials	188:218	polymer nanocomposite materials	188:218	Cellulose nanofibril (CNF) is a promising nanofiller for polymer nanocomposite materials, and a critical challenge in designing these materials is organization of the nanostructure using a facile process.
27958712	0	26	theme	Strong	29:34	arg1	Nanocomposites					80:93	Transparent, Strong, and Thermally Stable Nanocellulose/Polymer Nanocomposites	16:93	Transparent, Strong, and Thermally Stable Nanocellulose/Polymer Nanocomposites from an Aqueous Pickering Emulsion	16:128	Facile Route to Transparent, Strong, and Thermally Stable Nanocellulose/Polymer Nanocomposites from an Aqueous Pickering Emulsion.
27958712	6	27	theme	friendly	953:960	arg1	method					962:967	a simple and environmentally friendly method	924:967	a simple and environmentally friendly method for the preparation of novel CNF/polymer nanocomposite materials	924:1032	Thus, this technique provides a simple and environmentally friendly method for the preparation of novel CNF/polymer nanocomposite materials.
27958712	5	28	theme	thermal	863:869	arg1	stability					883:891	thermal dimensional stability	863:891	thermal dimensional stability	863:891	The PS/CNF nanocomposite showed high optical transparency, strength, and thermal dimensional stability.
27958712	2	29	theme	polystyrene	408:418	arg1	composites					429:438	nanostructured polystyrene (PS)/CNF composites	393:438	nanostructured polystyrene (PS)/CNF composites	393:438	Here, we report a facile aqueous preparation process for nanostructured polystyrene (PS)/CNF composites via the formation of a CNF-stabilized Pickering emulsion.
27958712	1	30	theme	nanostructure	298:310	arg1	nanofiller					173:182	a promising nanofiller	161:182	a promising nanofiller for polymer nanocomposite materials	161:218	Cellulose nanofibril (CNF) is a promising nanofiller for polymer nanocomposite materials, and a critical challenge in designing these materials is organization of the nanostructure using a facile process.
27958712	1	30	theme	nanostructure	298:310	arg1	challenge					236:244	a critical challenge	225:244	a critical challenge in designing these materials	225:273	Cellulose nanofibril (CNF) is a promising nanofiller for polymer nanocomposite materials, and a critical challenge in designing these materials is organization of the nanostructure using a facile process.
27958712	1	30	theme	nanostructure	298:310	arg1	organization					278:289	organization	278:289	organization of the nanostructure using a facile process	278:333	Cellulose nanofibril (CNF) is a promising nanofiller for polymer nanocomposite materials, and a critical challenge in designing these materials is organization of the nanostructure using a facile process.
27958712	1	30	theme	nanostructure	298:310	arg1	nanofibril					141:150	Cellulose nanofibril	131:150	Cellulose nanofibril (CNF)	131:156	Cellulose nanofibril (CNF) is a promising nanofiller for polymer nanocomposite materials, and a critical challenge in designing these materials is organization of the nanostructure using a facile process.
27958712	2	31	theme	/CNF	424:427	arg1	composites					429:438	nanostructured polystyrene (PS)/CNF composites	393:438	nanostructured polystyrene (PS)/CNF composites	393:438	Here, we report a facile aqueous preparation process for nanostructured polystyrene (PS)/CNF composites via the formation of a CNF-stabilized Pickering emulsion.
27958712	2	32	theme	nanostructured	393:406	arg1	composites					429:438	nanostructured polystyrene (PS)/CNF composites	393:438	nanostructured polystyrene (PS)/CNF composites	393:438	Here, we report a facile aqueous preparation process for nanostructured polystyrene (PS)/CNF composites via the formation of a CNF-stabilized Pickering emulsion.
27958712	5	33	theme	dimensional	871:881	arg1	stability					883:891	thermal dimensional stability	863:891	thermal dimensional stability	863:891	The PS/CNF nanocomposite showed high optical transparency, strength, and thermal dimensional stability.
27958712	3	34	theme	free	569:572	arg1	polymerization					582:595	free radical polymerization	569:595	free radical polymerization	569:595	PS nanoparticles, with a narrow size distribution, were synthesized by free radical polymerization in water using CNF as a stabilizer.
27958712	4	35	theme	resulting	696:704	arg1	material					706:713	the resulting material	692:713	the resulting material	692:713	The nanoparticles were easily collected by filtration, and the resulting material had a composite structure of PS nanoparticles embedded in a CNF framework.
27958712	6	36	theme	simple	926:931	arg1	method					962:967	a simple and environmentally friendly method	924:967	a simple and environmentally friendly method for the preparation of novel CNF/polymer nanocomposite materials	924:1032	Thus, this technique provides a simple and environmentally friendly method for the preparation of novel CNF/polymer nanocomposite materials.
27958712	3	37	theme	radical	574:580	arg1	polymerization					582:595	free radical polymerization	569:595	free radical polymerization	569:595	PS nanoparticles, with a narrow size distribution, were synthesized by free radical polymerization in water using CNF as a stabilizer.
27958712	1	38	theme	facile	320:325	arg1	process					327:333	a facile process	318:333	a facile process	318:333	Cellulose nanofibril (CNF) is a promising nanofiller for polymer nanocomposite materials, and a critical challenge in designing these materials is organization of the nanostructure using a facile process.
27958712	6	39	theme	environmentally	937:951	arg1	method					962:967	a simple and environmentally friendly method	924:967	a simple and environmentally friendly method for the preparation of novel CNF/polymer nanocomposite materials	924:1032	Thus, this technique provides a simple and environmentally friendly method for the preparation of novel CNF/polymer nanocomposite materials.
27958712	4	40	theme	CNF	775:777	arg1	framework					779:787	a CNF framework	773:787	a CNF framework	773:787	The nanoparticles were easily collected by filtration, and the resulting material had a composite structure of PS nanoparticles embedded in a CNF framework.
27958712	2	41	dep	preparation	369:379	arg1	process					381:387	process	381:387	process for nanostructured polystyrene (PS)/CNF composites via the formation of a CNF-stabilized Pickering emulsion	381:495	Here, we report a facile aqueous preparation process for nanostructured polystyrene (PS)/CNF composites via the formation of a CNF-stabilized Pickering emulsion.
27958712	1	42	theme	Cellulose	131:139	arg1	nanofiller					173:182	a promising nanofiller	161:182	a promising nanofiller for polymer nanocomposite materials	161:218	Cellulose nanofibril (CNF) is a promising nanofiller for polymer nanocomposite materials, and a critical challenge in designing these materials is organization of the nanostructure using a facile process.
27958712	1	42	theme	Cellulose	131:139	arg1	CNF					153:155	CNF	153:155	CNF	153:155	Cellulose nanofibril (CNF) is a promising nanofiller for polymer nanocomposite materials, and a critical challenge in designing these materials is organization of the nanostructure using a facile process.
27958712	1	42	theme	Cellulose	131:139	arg1	nanofibril					141:150	Cellulose nanofibril	131:150	Cellulose nanofibril (CNF)	131:156	Cellulose nanofibril (CNF) is a promising nanofiller for polymer nanocomposite materials, and a critical challenge in designing these materials is organization of the nanostructure using a facile process.
27958712	4	43	theme	embedded	761:768	arg1	nanoparticles					747:759	PS nanoparticles	744:759	PS nanoparticles embedded in a CNF framework	744:787	The nanoparticles were easily collected by filtration, and the resulting material had a composite structure of PS nanoparticles embedded in a CNF framework.
27958712	4	44	theme	nanoparticles	747:759	arg1	structure					731:739	a composite structure	719:739	a composite structure of PS nanoparticles embedded in a CNF framework	719:787	The nanoparticles were easily collected by filtration, and the resulting material had a composite structure of PS nanoparticles embedded in a CNF framework.
27958712	0	45	theme	Stable	51:56	arg1	Nanocomposites					80:93	Transparent, Strong, and Thermally Stable Nanocellulose/Polymer Nanocomposites	16:93	Transparent, Strong, and Thermally Stable Nanocellulose/Polymer Nanocomposites from an Aqueous Pickering Emulsion	16:128	Facile Route to Transparent, Strong, and Thermally Stable Nanocellulose/Polymer Nanocomposites from an Aqueous Pickering Emulsion.
27958712	1	46	theme	polymer	188:194	arg1	materials					210:218	polymer nanocomposite materials	188:218	polymer nanocomposite materials	188:218	Cellulose nanofibril (CNF) is a promising nanofiller for polymer nanocomposite materials, and a critical challenge in designing these materials is organization of the nanostructure using a facile process.
27958712	2	47	theme	facile	354:359	arg1	preparation					369:379	a facile aqueous preparation	352:379	a facile aqueous preparation process for nanostructured polystyrene (PS)/CNF composites via the formation of a CNF-stabilized Pickering emulsion	352:495	Here, we report a facile aqueous preparation process for nanostructured polystyrene (PS)/CNF composites via the formation of a CNF-stabilized Pickering emulsion.
27958712	1	48	theme	critical	227:234	arg1	challenge					236:244	a critical challenge	225:244	a critical challenge in designing these materials	225:273	Cellulose nanofibril (CNF) is a promising nanofiller for polymer nanocomposite materials, and a critical challenge in designing these materials is organization of the nanostructure using a facile process.
27958712	1	48	theme	critical	227:234	arg1	organization					278:289	organization	278:289	organization of the nanostructure using a facile process	278:333	Cellulose nanofibril (CNF) is a promising nanofiller for polymer nanocomposite materials, and a critical challenge in designing these materials is organization of the nanostructure using a facile process.
27958712	2	49	theme	emulsion	488:495	arg1	formation					448:456	the formation	444:456	the formation of a CNF-stabilized Pickering emulsion	444:495	Here, we report a facile aqueous preparation process for nanostructured polystyrene (PS)/CNF composites via the formation of a CNF-stabilized Pickering emulsion.
27958712	6	50	theme	nanocomposite	1010:1022	arg1	materials					1024:1032	novel CNF/polymer nanocomposite materials	992:1032	novel CNF/polymer nanocomposite materials	992:1032	Thus, this technique provides a simple and environmentally friendly method for the preparation of novel CNF/polymer nanocomposite materials.
27958712	0	51	from	Emulsion	121:128	arg1	Nanocomposites					80:93	Transparent, Strong, and Thermally Stable Nanocellulose/Polymer Nanocomposites	16:93	Transparent, Strong, and Thermally Stable Nanocellulose/Polymer Nanocomposites from an Aqueous Pickering Emulsion	16:128	Facile Route to Transparent, Strong, and Thermally Stable Nanocellulose/Polymer Nanocomposites from an Aqueous Pickering Emulsion.
28487197	4	0	theme	colorectal	685:694	arg1	cells					711:715	epithelial colorectal adenocarcinoma cells	674:715	epithelial colorectal adenocarcinoma cells (HT-29 cells)	674:729	All samples were extremely cytocompatible with many cell types, such as normal kidney epithelial cells (VERO cells), epithelial colorectal adenocarcinoma cells (HT-29 cells), HPV-16 positive human cervical tumour cells (SiHa cells), kidney epithelial cells (LLCMK2 cells) and murine macrophage cells (J774A1 cells).
28487197	4	0	theme	colorectal	685:694	arg1	cells					724:728	HT-29 cells	718:728	HT-29 cells	718:728	All samples were extremely cytocompatible with many cell types, such as normal kidney epithelial cells (VERO cells), epithelial colorectal adenocarcinoma cells (HT-29 cells), HPV-16 positive human cervical tumour cells (SiHa cells), kidney epithelial cells (LLCMK2 cells) and murine macrophage cells (J774A1 cells).
28487197	4	1	theme	macrophage	840:849	arg1	cells					851:855	murine macrophage cells	833:855	murine macrophage cells (J774A1 cells)	833:870	All samples were extremely cytocompatible with many cell types, such as normal kidney epithelial cells (VERO cells), epithelial colorectal adenocarcinoma cells (HT-29 cells), HPV-16 positive human cervical tumour cells (SiHa cells), kidney epithelial cells (LLCMK2 cells) and murine macrophage cells (J774A1 cells).
28487197	4	1	theme	macrophage	840:849	arg1	cells					865:869	J774A1 cells	858:869	J774A1 cells	858:869	All samples were extremely cytocompatible with many cell types, such as normal kidney epithelial cells (VERO cells), epithelial colorectal adenocarcinoma cells (HT-29 cells), HPV-16 positive human cervical tumour cells (SiHa cells), kidney epithelial cells (LLCMK2 cells) and murine macrophage cells (J774A1 cells).
28487197	4	2	theme	many	604:607	arg1	types					614:618	many cell types	604:618	many cell types	604:618	All samples were extremely cytocompatible with many cell types, such as normal kidney epithelial cells (VERO cells), epithelial colorectal adenocarcinoma cells (HT-29 cells), HPV-16 positive human cervical tumour cells (SiHa cells), kidney epithelial cells (LLCMK2 cells) and murine macrophage cells (J774A1 cells).
28487197	4	2	theme	many	604:607	arg1	cells					654:658	normal kidney epithelial cells	629:658	normal kidney epithelial cells (VERO cells)	629:671	All samples were extremely cytocompatible with many cell types, such as normal kidney epithelial cells (VERO cells), epithelial colorectal adenocarcinoma cells (HT-29 cells), HPV-16 positive human cervical tumour cells (SiHa cells), kidney epithelial cells (LLCMK2 cells) and murine macrophage cells (J774A1 cells).
28487197	4	2	theme	many	604:607	arg1	cells					770:774	HPV-16 positive human cervical tumour cells	732:774	HPV-16 positive human cervical tumour cells (SiHa cells)	732:787	All samples were extremely cytocompatible with many cell types, such as normal kidney epithelial cells (VERO cells), epithelial colorectal adenocarcinoma cells (HT-29 cells), HPV-16 positive human cervical tumour cells (SiHa cells), kidney epithelial cells (LLCMK2 cells) and murine macrophage cells (J774A1 cells).
28487197	4	2	theme	many	604:607	arg1	cells					711:715	epithelial colorectal adenocarcinoma cells	674:715	epithelial colorectal adenocarcinoma cells (HT-29 cells)	674:729	All samples were extremely cytocompatible with many cell types, such as normal kidney epithelial cells (VERO cells), epithelial colorectal adenocarcinoma cells (HT-29 cells), HPV-16 positive human cervical tumour cells (SiHa cells), kidney epithelial cells (LLCMK2 cells) and murine macrophage cells (J774A1 cells).
28487197	4	2	theme	many	604:607	arg1	cells					808:812	kidney epithelial cells	790:812	kidney epithelial cells (LLCMK2 cells)	790:827	All samples were extremely cytocompatible with many cell types, such as normal kidney epithelial cells (VERO cells), epithelial colorectal adenocarcinoma cells (HT-29 cells), HPV-16 positive human cervical tumour cells (SiHa cells), kidney epithelial cells (LLCMK2 cells) and murine macrophage cells (J774A1 cells).
28487197	4	2	theme	many	604:607	arg1	cells					851:855	murine macrophage cells	833:855	murine macrophage cells (J774A1 cells)	833:870	All samples were extremely cytocompatible with many cell types, such as normal kidney epithelial cells (VERO cells), epithelial colorectal adenocarcinoma cells (HT-29 cells), HPV-16 positive human cervical tumour cells (SiHa cells), kidney epithelial cells (LLCMK2 cells) and murine macrophage cells (J774A1 cells).
28487197	5	3	theme	viability	878:886	arg1	assays					888:893	Cell viability assays	873:893	Cell viability assays using the MTT method upon mouse preosteoblastic cells (MC3T3-E1 cells)	873:964	Cell viability assays using the MTT method upon mouse preosteoblastic cells (MC3T3-E1 cells) showed that CS/Pec and CS/Pec/AuNPs composites had the potential to foster proliferation and growth of bone cells, making them possible stimulators for reconstruction of bone tissues.
28487197	4	4	theme	murine	833:838	arg1	cells					851:855	murine macrophage cells	833:855	murine macrophage cells (J774A1 cells)	833:870	All samples were extremely cytocompatible with many cell types, such as normal kidney epithelial cells (VERO cells), epithelial colorectal adenocarcinoma cells (HT-29 cells), HPV-16 positive human cervical tumour cells (SiHa cells), kidney epithelial cells (LLCMK2 cells) and murine macrophage cells (J774A1 cells).
28487197	4	4	theme	murine	833:838	arg1	cells					865:869	J774A1 cells	858:869	J774A1 cells	858:869	All samples were extremely cytocompatible with many cell types, such as normal kidney epithelial cells (VERO cells), epithelial colorectal adenocarcinoma cells (HT-29 cells), HPV-16 positive human cervical tumour cells (SiHa cells), kidney epithelial cells (LLCMK2 cells) and murine macrophage cells (J774A1 cells).
28487197	0	5	contain	containing	61:70	arg1	hydrogels					51:59	chitosan/pectin thermosensitive hydrogels	19:59	chitosan/pectin thermosensitive hydrogels containing gold nanoparticles	19:89	Scaffolds based on chitosan/pectin thermosensitive hydrogels containing gold nanoparticles.
28487197	0	5	contain	containing	61:70	arg2	nanoparticles					77:89	gold nanoparticles	72:89	gold nanoparticles	72:89	Scaffolds based on chitosan/pectin thermosensitive hydrogels containing gold nanoparticles.
28487197	4	6	theme	adenocarcinoma	696:709	arg1	cells					711:715	epithelial colorectal adenocarcinoma cells	674:715	epithelial colorectal adenocarcinoma cells (HT-29 cells)	674:729	All samples were extremely cytocompatible with many cell types, such as normal kidney epithelial cells (VERO cells), epithelial colorectal adenocarcinoma cells (HT-29 cells), HPV-16 positive human cervical tumour cells (SiHa cells), kidney epithelial cells (LLCMK2 cells) and murine macrophage cells (J774A1 cells).
28487197	4	6	theme	adenocarcinoma	696:709	arg1	cells					724:728	HT-29 cells	718:728	HT-29 cells	718:728	All samples were extremely cytocompatible with many cell types, such as normal kidney epithelial cells (VERO cells), epithelial colorectal adenocarcinoma cells (HT-29 cells), HPV-16 positive human cervical tumour cells (SiHa cells), kidney epithelial cells (LLCMK2 cells) and murine macrophage cells (J774A1 cells).
28487197	5	7	theme	possible	1093:1100	arg1	stimulators					1102:1112	possible stimulators	1093:1112	possible stimulators for reconstruction of bone tissues	1093:1147	Cell viability assays using the MTT method upon mouse preosteoblastic cells (MC3T3-E1 cells) showed that CS/Pec and CS/Pec/AuNPs composites had the potential to foster proliferation and growth of bone cells, making them possible stimulators for reconstruction of bone tissues.
28487197	4	8	theme	tumour	763:768	arg1	cells					770:774	HPV-16 positive human cervical tumour cells	732:774	HPV-16 positive human cervical tumour cells (SiHa cells)	732:787	All samples were extremely cytocompatible with many cell types, such as normal kidney epithelial cells (VERO cells), epithelial colorectal adenocarcinoma cells (HT-29 cells), HPV-16 positive human cervical tumour cells (SiHa cells), kidney epithelial cells (LLCMK2 cells) and murine macrophage cells (J774A1 cells).
28487197	4	8	theme	tumour	763:768	arg1	cells					782:786	SiHa cells	777:786	SiHa cells	777:786	All samples were extremely cytocompatible with many cell types, such as normal kidney epithelial cells (VERO cells), epithelial colorectal adenocarcinoma cells (HT-29 cells), HPV-16 positive human cervical tumour cells (SiHa cells), kidney epithelial cells (LLCMK2 cells) and murine macrophage cells (J774A1 cells).
28487197	5	9	theme	mouse	921:925	arg1	cells					943:947	mouse preosteoblastic cells	921:947	mouse preosteoblastic cells (MC3T3-E1 cells)	921:964	Cell viability assays using the MTT method upon mouse preosteoblastic cells (MC3T3-E1 cells) showed that CS/Pec and CS/Pec/AuNPs composites had the potential to foster proliferation and growth of bone cells, making them possible stimulators for reconstruction of bone tissues.
28487197	5	9	theme	mouse	921:925	arg1	cells					959:963	MC3T3-E1 cells	950:963	MC3T3-E1 cells	950:963	Cell viability assays using the MTT method upon mouse preosteoblastic cells (MC3T3-E1 cells) showed that CS/Pec and CS/Pec/AuNPs composites had the potential to foster proliferation and growth of bone cells, making them possible stimulators for reconstruction of bone tissues.
28487197	4	10	theme	HPV-16	732:737	arg1	cells					770:774	HPV-16 positive human cervical tumour cells	732:774	HPV-16 positive human cervical tumour cells (SiHa cells)	732:787	All samples were extremely cytocompatible with many cell types, such as normal kidney epithelial cells (VERO cells), epithelial colorectal adenocarcinoma cells (HT-29 cells), HPV-16 positive human cervical tumour cells (SiHa cells), kidney epithelial cells (LLCMK2 cells) and murine macrophage cells (J774A1 cells).
28487197	4	10	theme	HPV-16	732:737	arg1	cells					782:786	SiHa cells	777:786	SiHa cells	777:786	All samples were extremely cytocompatible with many cell types, such as normal kidney epithelial cells (VERO cells), epithelial colorectal adenocarcinoma cells (HT-29 cells), HPV-16 positive human cervical tumour cells (SiHa cells), kidney epithelial cells (LLCMK2 cells) and murine macrophage cells (J774A1 cells).
28487197	5	11	theme	preosteoblastic	927:941	arg1	cells					943:947	mouse preosteoblastic cells	921:947	mouse preosteoblastic cells (MC3T3-E1 cells)	921:964	Cell viability assays using the MTT method upon mouse preosteoblastic cells (MC3T3-E1 cells) showed that CS/Pec and CS/Pec/AuNPs composites had the potential to foster proliferation and growth of bone cells, making them possible stimulators for reconstruction of bone tissues.
28487197	5	11	theme	preosteoblastic	927:941	arg1	cells					959:963	MC3T3-E1 cells	950:963	MC3T3-E1 cells	950:963	Cell viability assays using the MTT method upon mouse preosteoblastic cells (MC3T3-E1 cells) showed that CS/Pec and CS/Pec/AuNPs composites had the potential to foster proliferation and growth of bone cells, making them possible stimulators for reconstruction of bone tissues.
28487197	4	12	theme	cervical	754:761	arg1	cells					770:774	HPV-16 positive human cervical tumour cells	732:774	HPV-16 positive human cervical tumour cells (SiHa cells)	732:787	All samples were extremely cytocompatible with many cell types, such as normal kidney epithelial cells (VERO cells), epithelial colorectal adenocarcinoma cells (HT-29 cells), HPV-16 positive human cervical tumour cells (SiHa cells), kidney epithelial cells (LLCMK2 cells) and murine macrophage cells (J774A1 cells).
28487197	4	12	theme	cervical	754:761	arg1	cells					782:786	SiHa cells	777:786	SiHa cells	777:786	All samples were extremely cytocompatible with many cell types, such as normal kidney epithelial cells (VERO cells), epithelial colorectal adenocarcinoma cells (HT-29 cells), HPV-16 positive human cervical tumour cells (SiHa cells), kidney epithelial cells (LLCMK2 cells) and murine macrophage cells (J774A1 cells).
28487197	4	13	theme	cell	609:612	arg1	types					614:618	many cell types	604:618	many cell types	604:618	All samples were extremely cytocompatible with many cell types, such as normal kidney epithelial cells (VERO cells), epithelial colorectal adenocarcinoma cells (HT-29 cells), HPV-16 positive human cervical tumour cells (SiHa cells), kidney epithelial cells (LLCMK2 cells) and murine macrophage cells (J774A1 cells).
28487197	4	13	theme	cell	609:612	arg1	cells					654:658	normal kidney epithelial cells	629:658	normal kidney epithelial cells (VERO cells)	629:671	All samples were extremely cytocompatible with many cell types, such as normal kidney epithelial cells (VERO cells), epithelial colorectal adenocarcinoma cells (HT-29 cells), HPV-16 positive human cervical tumour cells (SiHa cells), kidney epithelial cells (LLCMK2 cells) and murine macrophage cells (J774A1 cells).
28487197	4	13	theme	cell	609:612	arg1	cells					770:774	HPV-16 positive human cervical tumour cells	732:774	HPV-16 positive human cervical tumour cells (SiHa cells)	732:787	All samples were extremely cytocompatible with many cell types, such as normal kidney epithelial cells (VERO cells), epithelial colorectal adenocarcinoma cells (HT-29 cells), HPV-16 positive human cervical tumour cells (SiHa cells), kidney epithelial cells (LLCMK2 cells) and murine macrophage cells (J774A1 cells).
28487197	4	13	theme	cell	609:612	arg1	cells					711:715	epithelial colorectal adenocarcinoma cells	674:715	epithelial colorectal adenocarcinoma cells (HT-29 cells)	674:729	All samples were extremely cytocompatible with many cell types, such as normal kidney epithelial cells (VERO cells), epithelial colorectal adenocarcinoma cells (HT-29 cells), HPV-16 positive human cervical tumour cells (SiHa cells), kidney epithelial cells (LLCMK2 cells) and murine macrophage cells (J774A1 cells).
28487197	4	13	theme	cell	609:612	arg1	cells					808:812	kidney epithelial cells	790:812	kidney epithelial cells (LLCMK2 cells)	790:827	All samples were extremely cytocompatible with many cell types, such as normal kidney epithelial cells (VERO cells), epithelial colorectal adenocarcinoma cells (HT-29 cells), HPV-16 positive human cervical tumour cells (SiHa cells), kidney epithelial cells (LLCMK2 cells) and murine macrophage cells (J774A1 cells).
28487197	4	13	theme	cell	609:612	arg1	cells					851:855	murine macrophage cells	833:855	murine macrophage cells (J774A1 cells)	833:870	All samples were extremely cytocompatible with many cell types, such as normal kidney epithelial cells (VERO cells), epithelial colorectal adenocarcinoma cells (HT-29 cells), HPV-16 positive human cervical tumour cells (SiHa cells), kidney epithelial cells (LLCMK2 cells) and murine macrophage cells (J774A1 cells).
28487197	4	14	theme	HT-29	718:722	arg1	cells					711:715	epithelial colorectal adenocarcinoma cells	674:715	epithelial colorectal adenocarcinoma cells (HT-29 cells)	674:729	All samples were extremely cytocompatible with many cell types, such as normal kidney epithelial cells (VERO cells), epithelial colorectal adenocarcinoma cells (HT-29 cells), HPV-16 positive human cervical tumour cells (SiHa cells), kidney epithelial cells (LLCMK2 cells) and murine macrophage cells (J774A1 cells).
28487197	4	14	theme	HT-29	718:722	arg1	cells					724:728	HT-29 cells	718:728	HT-29 cells	718:728	All samples were extremely cytocompatible with many cell types, such as normal kidney epithelial cells (VERO cells), epithelial colorectal adenocarcinoma cells (HT-29 cells), HPV-16 positive human cervical tumour cells (SiHa cells), kidney epithelial cells (LLCMK2 cells) and murine macrophage cells (J774A1 cells).
28487197	4	15	theme	kidney	636:641	arg1	cells					654:658	normal kidney epithelial cells	629:658	normal kidney epithelial cells (VERO cells)	629:671	All samples were extremely cytocompatible with many cell types, such as normal kidney epithelial cells (VERO cells), epithelial colorectal adenocarcinoma cells (HT-29 cells), HPV-16 positive human cervical tumour cells (SiHa cells), kidney epithelial cells (LLCMK2 cells) and murine macrophage cells (J774A1 cells).
28487197	4	15	theme	kidney	636:641	arg1	cells					666:670	VERO cells	661:670	VERO cells	661:670	All samples were extremely cytocompatible with many cell types, such as normal kidney epithelial cells (VERO cells), epithelial colorectal adenocarcinoma cells (HT-29 cells), HPV-16 positive human cervical tumour cells (SiHa cells), kidney epithelial cells (LLCMK2 cells) and murine macrophage cells (J774A1 cells).
28487197	1	16	theme	Thermosensitive	92:106	arg1	hydrogels					108:116	Thermosensitive hydrogels	92:116	Thermosensitive hydrogels based on chitosan/pectin (CS/Pec) and CS/Pec/gold nanoparticles (CS/Pec/AuNPs)	92:195	Thermosensitive hydrogels based on chitosan/pectin (CS/Pec) and CS/Pec/gold nanoparticles (CS/Pec/AuNPs) were successfully prepared with different AuNP levels.
28487197	5	17	theme	bone	1069:1072	arg1	cells					1074:1078	bone cells	1069:1078	bone cells	1069:1078	Cell viability assays using the MTT method upon mouse preosteoblastic cells (MC3T3-E1 cells) showed that CS/Pec and CS/Pec/AuNPs composites had the potential to foster proliferation and growth of bone cells, making them possible stimulators for reconstruction of bone tissues.
28487197	4	18	theme	LLCMK2	815:820	arg1	cells					808:812	kidney epithelial cells	790:812	kidney epithelial cells (LLCMK2 cells)	790:827	All samples were extremely cytocompatible with many cell types, such as normal kidney epithelial cells (VERO cells), epithelial colorectal adenocarcinoma cells (HT-29 cells), HPV-16 positive human cervical tumour cells (SiHa cells), kidney epithelial cells (LLCMK2 cells) and murine macrophage cells (J774A1 cells).
28487197	4	18	theme	LLCMK2	815:820	arg1	cells					822:826	LLCMK2 cells	815:826	LLCMK2 cells	815:826	All samples were extremely cytocompatible with many cell types, such as normal kidney epithelial cells (VERO cells), epithelial colorectal adenocarcinoma cells (HT-29 cells), HPV-16 positive human cervical tumour cells (SiHa cells), kidney epithelial cells (LLCMK2 cells) and murine macrophage cells (J774A1 cells).
28487197	4	19	with	cytocompatible	584:597	arg1	types					614:618	many cell types	604:618	many cell types	604:618	All samples were extremely cytocompatible with many cell types, such as normal kidney epithelial cells (VERO cells), epithelial colorectal adenocarcinoma cells (HT-29 cells), HPV-16 positive human cervical tumour cells (SiHa cells), kidney epithelial cells (LLCMK2 cells) and murine macrophage cells (J774A1 cells).
28487197	4	19	with	cytocompatible	584:597	arg1	cells					654:658	normal kidney epithelial cells	629:658	normal kidney epithelial cells (VERO cells)	629:671	All samples were extremely cytocompatible with many cell types, such as normal kidney epithelial cells (VERO cells), epithelial colorectal adenocarcinoma cells (HT-29 cells), HPV-16 positive human cervical tumour cells (SiHa cells), kidney epithelial cells (LLCMK2 cells) and murine macrophage cells (J774A1 cells).
28487197	4	19	with	cytocompatible	584:597	arg1	cells					770:774	HPV-16 positive human cervical tumour cells	732:774	HPV-16 positive human cervical tumour cells (SiHa cells)	732:787	All samples were extremely cytocompatible with many cell types, such as normal kidney epithelial cells (VERO cells), epithelial colorectal adenocarcinoma cells (HT-29 cells), HPV-16 positive human cervical tumour cells (SiHa cells), kidney epithelial cells (LLCMK2 cells) and murine macrophage cells (J774A1 cells).
28487197	4	19	with	cytocompatible	584:597	arg1	cells					711:715	epithelial colorectal adenocarcinoma cells	674:715	epithelial colorectal adenocarcinoma cells (HT-29 cells)	674:729	All samples were extremely cytocompatible with many cell types, such as normal kidney epithelial cells (VERO cells), epithelial colorectal adenocarcinoma cells (HT-29 cells), HPV-16 positive human cervical tumour cells (SiHa cells), kidney epithelial cells (LLCMK2 cells) and murine macrophage cells (J774A1 cells).
28487197	4	19	with	cytocompatible	584:597	arg1	cells					808:812	kidney epithelial cells	790:812	kidney epithelial cells (LLCMK2 cells)	790:827	All samples were extremely cytocompatible with many cell types, such as normal kidney epithelial cells (VERO cells), epithelial colorectal adenocarcinoma cells (HT-29 cells), HPV-16 positive human cervical tumour cells (SiHa cells), kidney epithelial cells (LLCMK2 cells) and murine macrophage cells (J774A1 cells).
28487197	4	19	with	cytocompatible	584:597	arg1	cells					851:855	murine macrophage cells	833:855	murine macrophage cells (J774A1 cells)	833:870	All samples were extremely cytocompatible with many cell types, such as normal kidney epithelial cells (VERO cells), epithelial colorectal adenocarcinoma cells (HT-29 cells), HPV-16 positive human cervical tumour cells (SiHa cells), kidney epithelial cells (LLCMK2 cells) and murine macrophage cells (J774A1 cells).
28487197	3	20	theme	UV-vis	471:476	arg1	techniques					478:487	UV-vis techniques	471:487	UV-vis techniques	471:487	The presence of AuNPs in the CS/Pec composite was evaluated via WAXS and UV-vis techniques, while SEM analysis assessed the average size of pores (350-600μm).
28487197	5	21	theme	cells	1074:1078	arg1	growth					1059:1064	growth	1059:1064	growth	1059:1064	Cell viability assays using the MTT method upon mouse preosteoblastic cells (MC3T3-E1 cells) showed that CS/Pec and CS/Pec/AuNPs composites had the potential to foster proliferation and growth of bone cells, making them possible stimulators for reconstruction of bone tissues.
28487197	5	21	theme	cells	1074:1078	arg1	proliferation					1041:1053	proliferation	1041:1053	proliferation	1041:1053	Cell viability assays using the MTT method upon mouse preosteoblastic cells (MC3T3-E1 cells) showed that CS/Pec and CS/Pec/AuNPs composites had the potential to foster proliferation and growth of bone cells, making them possible stimulators for reconstruction of bone tissues.
28487197	2	22	theme	AuNPs	345:349	arg1	amount					335:340	the amount	331:340	the amount of AuNPs	331:349	Using a tilting method, gelation temperature was demonstrated to decrease when the amount of AuNPs increased and pectin concentrations decreased.
28487197	2	22	theme	AuNPs	345:349	arg1	AuNPs					345:349	AuNPs	345:349	AuNPs	345:349	Using a tilting method, gelation temperature was demonstrated to decrease when the amount of AuNPs increased and pectin concentrations decreased.
28487197	5	23	theme	bone	1136:1139	arg1	tissues					1141:1147	bone tissues	1136:1147	bone tissues	1136:1147	Cell viability assays using the MTT method upon mouse preosteoblastic cells (MC3T3-E1 cells) showed that CS/Pec and CS/Pec/AuNPs composites had the potential to foster proliferation and growth of bone cells, making them possible stimulators for reconstruction of bone tissues.
28487197	5	24	theme	MTT	905:907	arg1	method					909:914	the MTT method	901:914	the MTT method	901:914	Cell viability assays using the MTT method upon mouse preosteoblastic cells (MC3T3-E1 cells) showed that CS/Pec and CS/Pec/AuNPs composites had the potential to foster proliferation and growth of bone cells, making them possible stimulators for reconstruction of bone tissues.
28487197	5	25	dep	CS/Pec	978:983	arg1	composites					1002:1011	composites	1002:1011	composites	1002:1011	Cell viability assays using the MTT method upon mouse preosteoblastic cells (MC3T3-E1 cells) showed that CS/Pec and CS/Pec/AuNPs composites had the potential to foster proliferation and growth of bone cells, making them possible stimulators for reconstruction of bone tissues.
28487197	0	26	theme	chitosan/pectin	19:33	arg1	hydrogels					51:59	chitosan/pectin thermosensitive hydrogels	19:59	chitosan/pectin thermosensitive hydrogels containing gold nanoparticles	19:89	Scaffolds based on chitosan/pectin thermosensitive hydrogels containing gold nanoparticles.
28487197	5	27	theme	tissues	1141:1147	arg1	reconstruction					1118:1131	reconstruction	1118:1131	reconstruction of bone tissues	1118:1147	Cell viability assays using the MTT method upon mouse preosteoblastic cells (MC3T3-E1 cells) showed that CS/Pec and CS/Pec/AuNPs composites had the potential to foster proliferation and growth of bone cells, making them possible stimulators for reconstruction of bone tissues.
28487197	4	28	theme	human	748:752	arg1	cells					770:774	HPV-16 positive human cervical tumour cells	732:774	HPV-16 positive human cervical tumour cells (SiHa cells)	732:787	All samples were extremely cytocompatible with many cell types, such as normal kidney epithelial cells (VERO cells), epithelial colorectal adenocarcinoma cells (HT-29 cells), HPV-16 positive human cervical tumour cells (SiHa cells), kidney epithelial cells (LLCMK2 cells) and murine macrophage cells (J774A1 cells).
28487197	4	28	theme	human	748:752	arg1	cells					782:786	SiHa cells	777:786	SiHa cells	777:786	All samples were extremely cytocompatible with many cell types, such as normal kidney epithelial cells (VERO cells), epithelial colorectal adenocarcinoma cells (HT-29 cells), HPV-16 positive human cervical tumour cells (SiHa cells), kidney epithelial cells (LLCMK2 cells) and murine macrophage cells (J774A1 cells).
28487197	1	29	theme	different	229:237	arg1	levels					244:249	different AuNP levels	229:249	different AuNP levels	229:249	Thermosensitive hydrogels based on chitosan/pectin (CS/Pec) and CS/Pec/gold nanoparticles (CS/Pec/AuNPs) were successfully prepared with different AuNP levels.
28487197	3	30	theme	AuNPs	414:418	arg1	presence					402:409	The presence	398:409	The presence of AuNPs in the CS/Pec composite	398:442	The presence of AuNPs in the CS/Pec composite was evaluated via WAXS and UV-vis techniques, while SEM analysis assessed the average size of pores (350-600μm).
28487197	4	31	theme	positive	739:746	arg1	cells					770:774	HPV-16 positive human cervical tumour cells	732:774	HPV-16 positive human cervical tumour cells (SiHa cells)	732:787	All samples were extremely cytocompatible with many cell types, such as normal kidney epithelial cells (VERO cells), epithelial colorectal adenocarcinoma cells (HT-29 cells), HPV-16 positive human cervical tumour cells (SiHa cells), kidney epithelial cells (LLCMK2 cells) and murine macrophage cells (J774A1 cells).
28487197	4	31	theme	positive	739:746	arg1	cells					782:786	SiHa cells	777:786	SiHa cells	777:786	All samples were extremely cytocompatible with many cell types, such as normal kidney epithelial cells (VERO cells), epithelial colorectal adenocarcinoma cells (HT-29 cells), HPV-16 positive human cervical tumour cells (SiHa cells), kidney epithelial cells (LLCMK2 cells) and murine macrophage cells (J774A1 cells).
28487197	5	32	contain	had	1013:1015	arg1	CS/Pec					978:983	CS/Pec	978:983	CS/Pec	978:983	Cell viability assays using the MTT method upon mouse preosteoblastic cells (MC3T3-E1 cells) showed that CS/Pec and CS/Pec/AuNPs composites had the potential to foster proliferation and growth of bone cells, making them possible stimulators for reconstruction of bone tissues.
28487197	5	32	contain	had	1013:1015	arg2	potential					1021:1029	the potential to foster proliferation and growth of bone cells	1017:1078	the potential to foster proliferation and growth of bone cells	1017:1078	Cell viability assays using the MTT method upon mouse preosteoblastic cells (MC3T3-E1 cells) showed that CS/Pec and CS/Pec/AuNPs composites had the potential to foster proliferation and growth of bone cells, making them possible stimulators for reconstruction of bone tissues.
28487197	5	32	contain	had	1013:1015	arg1	CS/Pec/AuNPs					989:1000	CS/Pec/AuNPs	989:1000	CS/Pec/AuNPs	989:1000	Cell viability assays using the MTT method upon mouse preosteoblastic cells (MC3T3-E1 cells) showed that CS/Pec and CS/Pec/AuNPs composites had the potential to foster proliferation and growth of bone cells, making them possible stimulators for reconstruction of bone tissues.
28487197	1	33	theme	AuNP	239:242	arg1	levels					244:249	different AuNP levels	229:249	different AuNP levels	229:249	Thermosensitive hydrogels based on chitosan/pectin (CS/Pec) and CS/Pec/gold nanoparticles (CS/Pec/AuNPs) were successfully prepared with different AuNP levels.
28487197	3	34	theme	average	522:528	arg1	size					530:533	the average size	518:533	the average size of pores (350-600μm)	518:554	The presence of AuNPs in the CS/Pec composite was evaluated via WAXS and UV-vis techniques, while SEM analysis assessed the average size of pores (350-600μm).
28487197	4	35	theme	epithelial	797:806	arg1	cells					808:812	kidney epithelial cells	790:812	kidney epithelial cells (LLCMK2 cells)	790:827	All samples were extremely cytocompatible with many cell types, such as normal kidney epithelial cells (VERO cells), epithelial colorectal adenocarcinoma cells (HT-29 cells), HPV-16 positive human cervical tumour cells (SiHa cells), kidney epithelial cells (LLCMK2 cells) and murine macrophage cells (J774A1 cells).
28487197	4	35	theme	epithelial	797:806	arg1	cells					822:826	LLCMK2 cells	815:826	LLCMK2 cells	815:826	All samples were extremely cytocompatible with many cell types, such as normal kidney epithelial cells (VERO cells), epithelial colorectal adenocarcinoma cells (HT-29 cells), HPV-16 positive human cervical tumour cells (SiHa cells), kidney epithelial cells (LLCMK2 cells) and murine macrophage cells (J774A1 cells).
28487197	0	36	theme	thermosensitive	35:49	arg1	hydrogels					51:59	chitosan/pectin thermosensitive hydrogels	19:59	chitosan/pectin thermosensitive hydrogels containing gold nanoparticles	19:89	Scaffolds based on chitosan/pectin thermosensitive hydrogels containing gold nanoparticles.
28487197	2	37	theme	tilting	260:266	arg1	method					268:273	a tilting method	258:273	a tilting method	258:273	Using a tilting method, gelation temperature was demonstrated to decrease when the amount of AuNPs increased and pectin concentrations decreased.
28487197	3	38	theme	CS/Pec	427:432	arg1	composite					434:442	the CS/Pec composite	423:442	the CS/Pec composite	423:442	The presence of AuNPs in the CS/Pec composite was evaluated via WAXS and UV-vis techniques, while SEM analysis assessed the average size of pores (350-600μm).
28487197	3	39	from	presence	402:409	arg1	composite					434:442	the CS/Pec composite	423:442	the CS/Pec composite	423:442	The presence of AuNPs in the CS/Pec composite was evaluated via WAXS and UV-vis techniques, while SEM analysis assessed the average size of pores (350-600μm).
28487197	3	40	theme	SEM	496:498	arg1	analysis					500:507	SEM analysis	496:507	SEM analysis	496:507	The presence of AuNPs in the CS/Pec composite was evaluated via WAXS and UV-vis techniques, while SEM analysis assessed the average size of pores (350-600μm).
28487197	4	41	theme	J774A1	858:863	arg1	cells					851:855	murine macrophage cells	833:855	murine macrophage cells (J774A1 cells)	833:870	All samples were extremely cytocompatible with many cell types, such as normal kidney epithelial cells (VERO cells), epithelial colorectal adenocarcinoma cells (HT-29 cells), HPV-16 positive human cervical tumour cells (SiHa cells), kidney epithelial cells (LLCMK2 cells) and murine macrophage cells (J774A1 cells).
28487197	4	41	theme	J774A1	858:863	arg1	cells					865:869	J774A1 cells	858:869	J774A1 cells	858:869	All samples were extremely cytocompatible with many cell types, such as normal kidney epithelial cells (VERO cells), epithelial colorectal adenocarcinoma cells (HT-29 cells), HPV-16 positive human cervical tumour cells (SiHa cells), kidney epithelial cells (LLCMK2 cells) and murine macrophage cells (J774A1 cells).
28487197	2	42	theme	pectin	365:370	arg1	concentrations					372:385	pectin concentrations	365:385	pectin concentrations	365:385	Using a tilting method, gelation temperature was demonstrated to decrease when the amount of AuNPs increased and pectin concentrations decreased.
28487197	5	43	theme	MC3T3-E1	950:957	arg1	cells					943:947	mouse preosteoblastic cells	921:947	mouse preosteoblastic cells (MC3T3-E1 cells)	921:964	Cell viability assays using the MTT method upon mouse preosteoblastic cells (MC3T3-E1 cells) showed that CS/Pec and CS/Pec/AuNPs composites had the potential to foster proliferation and growth of bone cells, making them possible stimulators for reconstruction of bone tissues.
28487197	5	43	theme	MC3T3-E1	950:957	arg1	cells					959:963	MC3T3-E1 cells	950:963	MC3T3-E1 cells	950:963	Cell viability assays using the MTT method upon mouse preosteoblastic cells (MC3T3-E1 cells) showed that CS/Pec and CS/Pec/AuNPs composites had the potential to foster proliferation and growth of bone cells, making them possible stimulators for reconstruction of bone tissues.
28487197	4	44	theme	kidney	790:795	arg1	cells					808:812	kidney epithelial cells	790:812	kidney epithelial cells (LLCMK2 cells)	790:827	All samples were extremely cytocompatible with many cell types, such as normal kidney epithelial cells (VERO cells), epithelial colorectal adenocarcinoma cells (HT-29 cells), HPV-16 positive human cervical tumour cells (SiHa cells), kidney epithelial cells (LLCMK2 cells) and murine macrophage cells (J774A1 cells).
28487197	4	44	theme	kidney	790:795	arg1	cells					822:826	LLCMK2 cells	815:826	LLCMK2 cells	815:826	All samples were extremely cytocompatible with many cell types, such as normal kidney epithelial cells (VERO cells), epithelial colorectal adenocarcinoma cells (HT-29 cells), HPV-16 positive human cervical tumour cells (SiHa cells), kidney epithelial cells (LLCMK2 cells) and murine macrophage cells (J774A1 cells).
28487197	5	45	theme	Cell	873:876	arg1	assays					888:893	Cell viability assays	873:893	Cell viability assays using the MTT method upon mouse preosteoblastic cells (MC3T3-E1 cells)	873:964	Cell viability assays using the MTT method upon mouse preosteoblastic cells (MC3T3-E1 cells) showed that CS/Pec and CS/Pec/AuNPs composites had the potential to foster proliferation and growth of bone cells, making them possible stimulators for reconstruction of bone tissues.
28487197	4	46	theme	SiHa	777:780	arg1	cells					770:774	HPV-16 positive human cervical tumour cells	732:774	HPV-16 positive human cervical tumour cells (SiHa cells)	732:787	All samples were extremely cytocompatible with many cell types, such as normal kidney epithelial cells (VERO cells), epithelial colorectal adenocarcinoma cells (HT-29 cells), HPV-16 positive human cervical tumour cells (SiHa cells), kidney epithelial cells (LLCMK2 cells) and murine macrophage cells (J774A1 cells).
28487197	4	46	theme	SiHa	777:780	arg1	cells					782:786	SiHa cells	777:786	SiHa cells	777:786	All samples were extremely cytocompatible with many cell types, such as normal kidney epithelial cells (VERO cells), epithelial colorectal adenocarcinoma cells (HT-29 cells), HPV-16 positive human cervical tumour cells (SiHa cells), kidney epithelial cells (LLCMK2 cells) and murine macrophage cells (J774A1 cells).
28487197	1	47	theme	CS/Pec/gold	156:166	arg1	CS/Pec/AuNPs					183:194	CS/Pec/AuNPs	183:194	CS/Pec/AuNPs	183:194	Thermosensitive hydrogels based on chitosan/pectin (CS/Pec) and CS/Pec/gold nanoparticles (CS/Pec/AuNPs) were successfully prepared with different AuNP levels.
28487197	1	47	theme	CS/Pec/gold	156:166	arg1	nanoparticles					168:180	CS/Pec/gold nanoparticles	156:180	CS/Pec/gold nanoparticles (CS/Pec/AuNPs)	156:195	Thermosensitive hydrogels based on chitosan/pectin (CS/Pec) and CS/Pec/gold nanoparticles (CS/Pec/AuNPs) were successfully prepared with different AuNP levels.
28487197	2	48	theme	gelation	276:283	arg1	temperature					285:295	gelation temperature	276:295	gelation temperature	276:295	Using a tilting method, gelation temperature was demonstrated to decrease when the amount of AuNPs increased and pectin concentrations decreased.
28487197	4	49	theme	epithelial	643:652	arg1	cells					654:658	normal kidney epithelial cells	629:658	normal kidney epithelial cells (VERO cells)	629:671	All samples were extremely cytocompatible with many cell types, such as normal kidney epithelial cells (VERO cells), epithelial colorectal adenocarcinoma cells (HT-29 cells), HPV-16 positive human cervical tumour cells (SiHa cells), kidney epithelial cells (LLCMK2 cells) and murine macrophage cells (J774A1 cells).
28487197	4	49	theme	epithelial	643:652	arg1	cells					666:670	VERO cells	661:670	VERO cells	661:670	All samples were extremely cytocompatible with many cell types, such as normal kidney epithelial cells (VERO cells), epithelial colorectal adenocarcinoma cells (HT-29 cells), HPV-16 positive human cervical tumour cells (SiHa cells), kidney epithelial cells (LLCMK2 cells) and murine macrophage cells (J774A1 cells).
28487197	3	50	theme	pores	538:542	arg1	size					530:533	the average size	518:533	the average size of pores (350-600μm)	518:554	The presence of AuNPs in the CS/Pec composite was evaluated via WAXS and UV-vis techniques, while SEM analysis assessed the average size of pores (350-600μm).
28487197	4	51	theme	VERO	661:664	arg1	cells					654:658	normal kidney epithelial cells	629:658	normal kidney epithelial cells (VERO cells)	629:671	All samples were extremely cytocompatible with many cell types, such as normal kidney epithelial cells (VERO cells), epithelial colorectal adenocarcinoma cells (HT-29 cells), HPV-16 positive human cervical tumour cells (SiHa cells), kidney epithelial cells (LLCMK2 cells) and murine macrophage cells (J774A1 cells).
28487197	4	51	theme	VERO	661:664	arg1	cells					666:670	VERO cells	661:670	VERO cells	661:670	All samples were extremely cytocompatible with many cell types, such as normal kidney epithelial cells (VERO cells), epithelial colorectal adenocarcinoma cells (HT-29 cells), HPV-16 positive human cervical tumour cells (SiHa cells), kidney epithelial cells (LLCMK2 cells) and murine macrophage cells (J774A1 cells).
28487197	4	52	theme	normal	629:634	arg1	cells					654:658	normal kidney epithelial cells	629:658	normal kidney epithelial cells (VERO cells)	629:671	All samples were extremely cytocompatible with many cell types, such as normal kidney epithelial cells (VERO cells), epithelial colorectal adenocarcinoma cells (HT-29 cells), HPV-16 positive human cervical tumour cells (SiHa cells), kidney epithelial cells (LLCMK2 cells) and murine macrophage cells (J774A1 cells).
28487197	4	52	theme	normal	629:634	arg1	cells					666:670	VERO cells	661:670	VERO cells	661:670	All samples were extremely cytocompatible with many cell types, such as normal kidney epithelial cells (VERO cells), epithelial colorectal adenocarcinoma cells (HT-29 cells), HPV-16 positive human cervical tumour cells (SiHa cells), kidney epithelial cells (LLCMK2 cells) and murine macrophage cells (J774A1 cells).
28487197	0	53	theme	gold	72:75	arg1	nanoparticles					77:89	gold nanoparticles	72:89	gold nanoparticles	72:89	Scaffolds based on chitosan/pectin thermosensitive hydrogels containing gold nanoparticles.
28487197	3	54	attach	presence	402:409	arg1	composite					434:442	the CS/Pec composite	423:442	the CS/Pec composite	423:442	The presence of AuNPs in the CS/Pec composite was evaluated via WAXS and UV-vis techniques, while SEM analysis assessed the average size of pores (350-600μm).
28487197	3	54	attach	presence	402:409	arg2	AuNPs					414:418	AuNPs	414:418	AuNPs	414:418	The presence of AuNPs in the CS/Pec composite was evaluated via WAXS and UV-vis techniques, while SEM analysis assessed the average size of pores (350-600μm).
28487197	4	55	theme	epithelial	674:683	arg1	cells					711:715	epithelial colorectal adenocarcinoma cells	674:715	epithelial colorectal adenocarcinoma cells (HT-29 cells)	674:729	All samples were extremely cytocompatible with many cell types, such as normal kidney epithelial cells (VERO cells), epithelial colorectal adenocarcinoma cells (HT-29 cells), HPV-16 positive human cervical tumour cells (SiHa cells), kidney epithelial cells (LLCMK2 cells) and murine macrophage cells (J774A1 cells).
28487197	4	55	theme	epithelial	674:683	arg1	cells					724:728	HT-29 cells	718:728	HT-29 cells	718:728	All samples were extremely cytocompatible with many cell types, such as normal kidney epithelial cells (VERO cells), epithelial colorectal adenocarcinoma cells (HT-29 cells), HPV-16 positive human cervical tumour cells (SiHa cells), kidney epithelial cells (LLCMK2 cells) and murine macrophage cells (J774A1 cells).
28103425	6	0	theme	azide	1004:1008	arg1	presence					988:995	the presence	984:995	the presence of the azide	984:1008	Kinetic analysis suggests that poor transglycosylation in the presence of the azide is because of low activity of the ternary complex between enzyme, β-galactosyl azide and acceptor.
28103425	10	1	theme	α-galactosidase	1591:1605	arg1	Trp312					1577:1582	Trp312	1577:1582	Trp312 of the α-galactosidase	1577:1605	Trp312 of the α-galactosidase appears to exclude the equatorial hydroxy group at C4 of glucosides, whereas the corresponding Trp residue in the α-glucoside hydrolase makes a hydrogen bond with this hydroxy group.
28103425	10	2	theme	hydroxy	1775:1781	arg1	group					1783:1787	this hydroxy group	1770:1787	this hydroxy group	1770:1787	Trp312 of the α-galactosidase appears to exclude the equatorial hydroxy group at C4 of glucosides, whereas the corresponding Trp residue in the α-glucoside hydrolase makes a hydrogen bond with this hydroxy group.
28103425	7	3	from	enzyme	1153:1158	arg1	complex					1163:1169	complex	1163:1169	complex with the transglycosylation product, β-lactosyl α-d-galactoside,	1163:1234	A three-dimensional structure of the mutant enzyme in complex with the transglycosylation product, β-lactosyl α-d-galactoside, was solved to elucidate the ligand-binding aspects of the α-galactosidase.
28103425	6	4	from	transglycosylation	962:979	arg1	presence					988:995	the presence	984:995	the presence of the azide	984:1008	Kinetic analysis suggests that poor transglycosylation in the presence of the azide is because of low activity of the ternary complex between enzyme, β-galactosyl azide and acceptor.
28103425	3	5	theme	GH	592:593	arg1	α-galactosidase					599:613	a glycoside hydrolase family (GH) 97 α-galactosidase	562:613	a glycoside hydrolase family (GH) 97 α-galactosidase	562:613	Here, we investigate a transglycosylation catalyzed by a catalytic nucleophile mutant derived from a glycoside hydrolase family (GH) 97 α-galactosidase, using more stable β-galactosyl azide and α-galactosyl fluoride donors.
28103425	1	6	theme	glycosidic	281:290	arg1	linkages					292:299	glycosidic linkages	281:299	glycosidic linkages	281:299	The preparation of a glycosynthase, a catalytic nucleophile mutant of a glycosidase, is a well-established strategy for the effective synthesis of glycosidic linkages.
28103425	8	7	theme	catalytic	1365:1373	arg1	barrel					1379:1384	the catalytic TIM barrel	1361:1384	the catalytic TIM barrel of the α-galactosidase from those of a homologous GH97 α-glucoside hydrolase	1361:1461	Subtle differences at the β→α loops 1, 2 and 3 of the catalytic TIM barrel of the α-galactosidase from those of a homologous GH97 α-glucoside hydrolase seem to be involved in substrate recognitions.
28103425	7	8	theme	transglycosylation	1180:1197	arg1	product					1199:1205	the transglycosylation product	1176:1205	the transglycosylation product	1176:1205	A three-dimensional structure of the mutant enzyme in complex with the transglycosylation product, β-lactosyl α-d-galactoside, was solved to elucidate the ligand-binding aspects of the α-galactosidase.
28103425	7	8	theme	transglycosylation	1180:1197	arg1	α-d-galactoside					1219:1233	β-lactosyl α-d-galactoside	1208:1233	β-lactosyl α-d-galactoside	1208:1233	A three-dimensional structure of the mutant enzyme in complex with the transglycosylation product, β-lactosyl α-d-galactoside, was solved to elucidate the ligand-binding aspects of the α-galactosidase.
28103425	3	9	theme	family	584:589	arg1	α-galactosidase					599:613	a glycoside hydrolase family (GH) 97 α-galactosidase	562:613	a glycoside hydrolase family (GH) 97 α-galactosidase	562:613	Here, we investigate a transglycosylation catalyzed by a catalytic nucleophile mutant derived from a glycoside hydrolase family (GH) 97 α-galactosidase, using more stable β-galactosyl azide and α-galactosyl fluoride donors.
28103425	12	10	dep	DATABASE	1890:1897	arg1	code					1923:1926	code	1923:1926	code	1923:1926	DATABASE The atomic coordinates (code: 5E1Q) have been deposited in the Protein Data Bank.
28103425	12	10	dep	DATABASE	1890:1897	arg1	coordinates					1910:1920	The atomic coordinates	1899:1920	DATABASE The atomic coordinates (code: 5E1Q)	1890:1933	DATABASE The atomic coordinates (code: 5E1Q) have been deposited in the Protein Data Bank.
28103425	4	11	theme	glycosynthase	719:731	arg1	reaction					733:740	the glycosynthase reaction	715:740	the glycosynthase reaction using β-galactosyl azide and α-galactosyl transfer from α-galactosyl fluoride with assistance of external anions	715:853	The mutant enzyme catalyzes the glycosynthase reaction using β-galactosyl azide and α-galactosyl transfer from α-galactosyl fluoride with assistance of external anions.
28103425	9	12	theme	Trp	1529:1531	arg1	residues					1533:1540	the Trp residues	1525:1540	the Trp residues in β→α loop 1	1525:1554	In particular, the Trp residues in β→α loop 1 have separate roles.
28103425	6	13	theme	ternary	1044:1050	arg1	complex					1052:1058	the ternary complex	1040:1058	the ternary complex between enzyme, β-galactosyl azide and acceptor	1040:1106	Kinetic analysis suggests that poor transglycosylation in the presence of the azide is because of low activity of the ternary complex between enzyme, β-galactosyl azide and acceptor.
28103425	8	14	theme	GH97	1436:1439	arg1	hydrolase					1453:1461	a homologous GH97 α-glucoside hydrolase	1423:1461	a homologous GH97 α-glucoside hydrolase	1423:1461	Subtle differences at the β→α loops 1, 2 and 3 of the catalytic TIM barrel of the α-galactosidase from those of a homologous GH97 α-glucoside hydrolase seem to be involved in substrate recognitions.
28103425	2	15	attach	derived	326:332	arg2	glycosynthases					311:324	glycosynthases	311:324	glycosynthases derived from α-glycosidases	311:352	However, glycosynthases derived from α-glycosidases can give poor yields of desired products because they require generally unstable β-glycosyl fluoride donors.
28103425	2	15	attach	derived	326:332	arg1	α-glycosidases					339:352	α-glycosidases	339:352	α-glycosidases	339:352	However, glycosynthases derived from α-glycosidases can give poor yields of desired products because they require generally unstable β-glycosyl fluoride donors.
28103425	4	16	theme	mutant	691:696	arg1	enzyme					698:703	The mutant enzyme	687:703	The mutant enzyme	687:703	The mutant enzyme catalyzes the glycosynthase reaction using β-galactosyl azide and α-galactosyl transfer from α-galactosyl fluoride with assistance of external anions.
28103425	7	17	theme	mutant	1146:1151	arg1	enzyme					1153:1158	the mutant enzyme	1142:1158	the mutant enzyme in complex with the transglycosylation product, β-lactosyl α-d-galactoside,	1142:1234	A three-dimensional structure of the mutant enzyme in complex with the transglycosylation product, β-lactosyl α-d-galactoside, was solved to elucidate the ligand-binding aspects of the α-galactosidase.
28103425	5	18	theme	transfer	896:903	arg1	activity					905:912	transfer activity	896:912	transfer activity than azide	896:923	Formate was more effective at restoring transfer activity than azide.
28103425	6	19	theme	low	1024:1026	arg1	activity					1028:1035	low activity	1024:1035	low activity of the ternary complex between enzyme, β-galactosyl azide and acceptor	1024:1106	Kinetic analysis suggests that poor transglycosylation in the presence of the azide is because of low activity of the ternary complex between enzyme, β-galactosyl azide and acceptor.
28103425	4	20	theme	α-galactosyl	771:782	arg1	transfer					784:791	α-galactosyl transfer	771:791	α-galactosyl transfer	771:791	The mutant enzyme catalyzes the glycosynthase reaction using β-galactosyl azide and α-galactosyl transfer from α-galactosyl fluoride with assistance of external anions.
28103425	4	21	with	fluoride	811:818	arg1	assistance					825:834	assistance	825:834	assistance of external anions	825:853	The mutant enzyme catalyzes the glycosynthase reaction using β-galactosyl azide and α-galactosyl transfer from α-galactosyl fluoride with assistance of external anions.
28103425	8	22	from	loops	1341:1345	arg1	differences					1318:1328	Subtle differences	1311:1328	Subtle differences at the β→α loops 1, 2 and 3 of the catalytic TIM barrel of the α-galactosidase from those of a homologous GH97 α-glucoside hydrolase	1311:1461	Subtle differences at the β→α loops 1, 2 and 3 of the catalytic TIM barrel of the α-galactosidase from those of a homologous GH97 α-glucoside hydrolase seem to be involved in substrate recognitions.
28103425	8	23	theme	barrel	1379:1384	arg1	loops					1341:1345	the β→α loops 1, 2 and 3	1333:1356	the β→α loops 1, 2 and 3 of the catalytic TIM barrel of the α-galactosidase from those of a homologous GH97 α-glucoside hydrolase	1333:1461	Subtle differences at the β→α loops 1, 2 and 3 of the catalytic TIM barrel of the α-galactosidase from those of a homologous GH97 α-glucoside hydrolase seem to be involved in substrate recognitions.
28103425	11	24	theme	α-galactoside	1807:1819	arg1	recognition					1821:1831	α-galactoside recognition	1807:1831	α-galactoside recognition	1807:1831	The mechanism of α-galactoside recognition is conserved among GH27, 31, 36 and 97 α-galactosidases.
28103425	7	25	theme	three-dimensional	1111:1127	arg1	structure					1129:1137	A three-dimensional structure	1109:1137	A three-dimensional structure of the mutant enzyme in complex with the transglycosylation product, β-lactosyl α-d-galactoside,	1109:1234	A three-dimensional structure of the mutant enzyme in complex with the transglycosylation product, β-lactosyl α-d-galactoside, was solved to elucidate the ligand-binding aspects of the α-galactosidase.
28103425	3	26	theme	fluoride	670:677	arg1	donors					679:684	more stable β-galactosyl azide and α-galactosyl fluoride donors	622:684	donors	679:684	Here, we investigate a transglycosylation catalyzed by a catalytic nucleophile mutant derived from a glycoside hydrolase family (GH) 97 α-galactosidase, using more stable β-galactosyl azide and α-galactosyl fluoride donors.
28103425	0	27	theme	Bacteroides	68:78	arg1	thetaiotaomicron					80:95	a mutant Bacteroides thetaiotaomicron	59:95	a mutant Bacteroides thetaiotaomicron	59:95	Efficient synthesis of α-galactosyl oligosaccharides using a mutant Bacteroides thetaiotaomicron retaining α-galactosidase (BtGH97b).
28103425	10	28	theme	hydroxy	1641:1647	arg1	glucosides					1664:1673	glucosides	1664:1673	glucosides	1664:1673	Trp312 of the α-galactosidase appears to exclude the equatorial hydroxy group at C4 of glucosides, whereas the corresponding Trp residue in the α-glucoside hydrolase makes a hydrogen bond with this hydroxy group.
28103425	10	28	theme	hydroxy	1641:1647	arg1	group					1649:1653	the equatorial hydroxy group	1626:1653	the equatorial hydroxy group at C4 of glucosides	1626:1673	Trp312 of the α-galactosidase appears to exclude the equatorial hydroxy group at C4 of glucosides, whereas the corresponding Trp residue in the α-glucoside hydrolase makes a hydrogen bond with this hydroxy group.
28103425	8	29	from	barrel	1379:1384	arg1	those					1414:1418	those	1414:1418	those	1414:1418	Subtle differences at the β→α loops 1, 2 and 3 of the catalytic TIM barrel of the α-galactosidase from those of a homologous GH97 α-glucoside hydrolase seem to be involved in substrate recognitions.
28103425	1	30	theme	catalytic	172:180	arg1	mutant					194:199	a catalytic nucleophile mutant	170:199	a catalytic nucleophile mutant of a glycosidase	170:216	The preparation of a glycosynthase, a catalytic nucleophile mutant of a glycosidase, is a well-established strategy for the effective synthesis of glycosidic linkages.
28103425	1	30	theme	catalytic	172:180	arg1	glycosynthase					155:167	a glycosynthase	153:167	a glycosynthase	153:167	The preparation of a glycosynthase, a catalytic nucleophile mutant of a glycosidase, is a well-established strategy for the effective synthesis of glycosidic linkages.
28103425	10	31	with	bond	1760:1763	arg1	group					1783:1787	this hydroxy group	1770:1787	this hydroxy group	1770:1787	Trp312 of the α-galactosidase appears to exclude the equatorial hydroxy group at C4 of glucosides, whereas the corresponding Trp residue in the α-glucoside hydrolase makes a hydrogen bond with this hydroxy group.
28103425	4	32	theme	external	839:846	arg1	anions					848:853	external anions	839:853	external anions	839:853	The mutant enzyme catalyzes the glycosynthase reaction using β-galactosyl azide and α-galactosyl transfer from α-galactosyl fluoride with assistance of external anions.
28103425	12	33	theme	Protein	1962:1968	arg1	Bank					1975:1978	the Protein Data Bank	1958:1978	the Protein Data Bank	1958:1978	DATABASE The atomic coordinates (code: 5E1Q) have been deposited in the Protein Data Bank.
28103425	8	34	theme	homologous	1425:1434	arg1	hydrolase					1453:1461	a homologous GH97 α-glucoside hydrolase	1423:1461	a homologous GH97 α-glucoside hydrolase	1423:1461	Subtle differences at the β→α loops 1, 2 and 3 of the catalytic TIM barrel of the α-galactosidase from those of a homologous GH97 α-glucoside hydrolase seem to be involved in substrate recognitions.
28103425	2	35	theme	unstable	426:433	arg1	donors					455:460	generally unstable β-glycosyl fluoride donors	416:460	generally unstable β-glycosyl fluoride donors	416:460	However, glycosynthases derived from α-glycosidases can give poor yields of desired products because they require generally unstable β-glycosyl fluoride donors.
28103425	12	36	dep	code	1923:1926	arg1	5E1Q					1929:1932	5E1Q	1929:1932	5E1Q	1929:1932	DATABASE The atomic coordinates (code: 5E1Q) have been deposited in the Protein Data Bank.
28103425	8	37	theme	α-glucoside	1441:1451	arg1	hydrolase					1453:1461	a homologous GH97 α-glucoside hydrolase	1423:1461	a homologous GH97 α-glucoside hydrolase	1423:1461	Subtle differences at the β→α loops 1, 2 and 3 of the catalytic TIM barrel of the α-galactosidase from those of a homologous GH97 α-glucoside hydrolase seem to be involved in substrate recognitions.
28103425	0	38	theme	Efficient	0:8	arg1	synthesis					10:18	Efficient synthesis	0:18	Efficient synthesis of α-galactosyl oligosaccharides using a mutant Bacteroides thetaiotaomicron	0:95	Efficient synthesis of α-galactosyl oligosaccharides using a mutant Bacteroides thetaiotaomicron retaining α-galactosidase (BtGH97b).
28103425	1	39	theme	linkages	292:299	arg1	synthesis					268:276	the effective synthesis	254:276	the effective synthesis of glycosidic linkages	254:299	The preparation of a glycosynthase, a catalytic nucleophile mutant of a glycosidase, is a well-established strategy for the effective synthesis of glycosidic linkages.
28103425	6	40	theme	poor	957:960	arg1	transglycosylation					962:979	poor transglycosylation	957:979	poor transglycosylation in the presence of the azide	957:1008	Kinetic analysis suggests that poor transglycosylation in the presence of the azide is because of low activity of the ternary complex between enzyme, β-galactosyl azide and acceptor.
28103425	9	41	contain	have	1556:1559	arg2	roles					1570:1574	separate roles	1561:1574	separate roles	1561:1574	In particular, the Trp residues in β→α loop 1 have separate roles.
28103425	9	41	contain	have	1556:1559	arg1	residues					1533:1540	the Trp residues	1525:1540	the Trp residues in β→α loop 1	1525:1554	In particular, the Trp residues in β→α loop 1 have separate roles.
28103425	2	42	theme	fluoride	446:453	arg1	donors					455:460	generally unstable β-glycosyl fluoride donors	416:460	generally unstable β-glycosyl fluoride donors	416:460	However, glycosynthases derived from α-glycosidases can give poor yields of desired products because they require generally unstable β-glycosyl fluoride donors.
28103425	0	43	theme	oligosaccharides	36:51	arg1	synthesis					10:18	Efficient synthesis	0:18	Efficient synthesis of α-galactosyl oligosaccharides using a mutant Bacteroides thetaiotaomicron	0:95	Efficient synthesis of α-galactosyl oligosaccharides using a mutant Bacteroides thetaiotaomicron retaining α-galactosidase (BtGH97b).
28103425	10	44	from	residue	1706:1712	arg1	hydrolase					1733:1741	the α-glucoside hydrolase	1717:1741	the α-glucoside hydrolase	1717:1741	Trp312 of the α-galactosidase appears to exclude the equatorial hydroxy group at C4 of glucosides, whereas the corresponding Trp residue in the α-glucoside hydrolase makes a hydrogen bond with this hydroxy group.
28103425	3	45	theme	nucleophile	530:540	arg1	mutant					542:547	a catalytic nucleophile mutant	518:547	a catalytic nucleophile mutant derived from a glycoside hydrolase family (GH) 97 α-galactosidase, using more stable β-galactosyl azide and α-galactosyl fluoride donors	518:684	Here, we investigate a transglycosylation catalyzed by a catalytic nucleophile mutant derived from a glycoside hydrolase family (GH) 97 α-galactosidase, using more stable β-galactosyl azide and α-galactosyl fluoride donors.
28103425	6	46	theme	Kinetic	926:932	arg1	analysis					934:941	Kinetic analysis	926:941	Kinetic analysis	926:941	Kinetic analysis suggests that poor transglycosylation in the presence of the azide is because of low activity of the ternary complex between enzyme, β-galactosyl azide and acceptor.
28103425	9	47	from	residues	1533:1540	arg1	loop					1549:1552	β→α loop 1	1545:1554	β→α loop 1	1545:1554	In particular, the Trp residues in β→α loop 1 have separate roles.
28103425	1	48	theme	well-established	224:239	arg1	strategy					241:248	a well-established strategy	222:248	a well-established strategy for the effective synthesis of glycosidic linkages	222:299	The preparation of a glycosynthase, a catalytic nucleophile mutant of a glycosidase, is a well-established strategy for the effective synthesis of glycosidic linkages.
28103425	1	48	theme	well-established	224:239	arg1	preparation					138:148	The preparation	134:148	The preparation of a glycosynthase, a catalytic nucleophile mutant of a glycosidase,	134:217	The preparation of a glycosynthase, a catalytic nucleophile mutant of a glycosidase, is a well-established strategy for the effective synthesis of glycosidic linkages.
28103425	12	49	theme	atomic	1903:1908	arg1	code					1923:1926	code	1923:1926	code	1923:1926	DATABASE The atomic coordinates (code: 5E1Q) have been deposited in the Protein Data Bank.
28103425	12	49	theme	atomic	1903:1908	arg1	coordinates					1910:1920	The atomic coordinates	1899:1920	DATABASE The atomic coordinates (code: 5E1Q)	1890:1933	DATABASE The atomic coordinates (code: 5E1Q) have been deposited in the Protein Data Bank.
28103425	10	50	theme	α-glucoside	1721:1731	arg1	hydrolase					1733:1741	the α-glucoside hydrolase	1717:1741	the α-glucoside hydrolase	1717:1741	Trp312 of the α-galactosidase appears to exclude the equatorial hydroxy group at C4 of glucosides, whereas the corresponding Trp residue in the α-glucoside hydrolase makes a hydrogen bond with this hydroxy group.
28103425	8	51	from	those	1414:1418	arg1	barrel					1379:1384	the catalytic TIM barrel	1361:1384	the catalytic TIM barrel of the α-galactosidase from those of a homologous GH97 α-glucoside hydrolase	1361:1461	Subtle differences at the β→α loops 1, 2 and 3 of the catalytic TIM barrel of the α-galactosidase from those of a homologous GH97 α-glucoside hydrolase seem to be involved in substrate recognitions.
28103425	8	51	from	those	1414:1418	arg1	α-galactosidase					1393:1407	the α-galactosidase	1389:1407	the α-galactosidase from those of a homologous GH97 α-glucoside hydrolase	1389:1461	Subtle differences at the β→α loops 1, 2 and 3 of the catalytic TIM barrel of the α-galactosidase from those of a homologous GH97 α-glucoside hydrolase seem to be involved in substrate recognitions.
28103425	10	52	from	C4	1658:1659	arg1	glucosides					1664:1673	glucosides	1664:1673	glucosides	1664:1673	Trp312 of the α-galactosidase appears to exclude the equatorial hydroxy group at C4 of glucosides, whereas the corresponding Trp residue in the α-glucoside hydrolase makes a hydrogen bond with this hydroxy group.
28103425	10	52	from	C4	1658:1659	arg1	group					1649:1653	the equatorial hydroxy group	1626:1653	the equatorial hydroxy group at C4 of glucosides	1626:1673	Trp312 of the α-galactosidase appears to exclude the equatorial hydroxy group at C4 of glucosides, whereas the corresponding Trp residue in the α-glucoside hydrolase makes a hydrogen bond with this hydroxy group.
28103425	7	53	theme	β-lactosyl	1208:1217	arg1	product					1199:1205	the transglycosylation product	1176:1205	the transglycosylation product	1176:1205	A three-dimensional structure of the mutant enzyme in complex with the transglycosylation product, β-lactosyl α-d-galactoside, was solved to elucidate the ligand-binding aspects of the α-galactosidase.
28103425	7	53	theme	β-lactosyl	1208:1217	arg1	α-d-galactoside					1219:1233	β-lactosyl α-d-galactoside	1208:1233	β-lactosyl α-d-galactoside	1208:1233	A three-dimensional structure of the mutant enzyme in complex with the transglycosylation product, β-lactosyl α-d-galactoside, was solved to elucidate the ligand-binding aspects of the α-galactosidase.
28103425	2	54	theme	desired	378:384	arg1	products					386:393	desired products	378:393	desired products	378:393	However, glycosynthases derived from α-glycosidases can give poor yields of desired products because they require generally unstable β-glycosyl fluoride donors.
28103425	1	55	theme	effective	258:266	arg1	synthesis					268:276	the effective synthesis	254:276	the effective synthesis of glycosidic linkages	254:299	The preparation of a glycosynthase, a catalytic nucleophile mutant of a glycosidase, is a well-established strategy for the effective synthesis of glycosidic linkages.
28103425	10	56	theme	equatorial	1630:1639	arg1	glucosides					1664:1673	glucosides	1664:1673	glucosides	1664:1673	Trp312 of the α-galactosidase appears to exclude the equatorial hydroxy group at C4 of glucosides, whereas the corresponding Trp residue in the α-glucoside hydrolase makes a hydrogen bond with this hydroxy group.
28103425	10	56	theme	equatorial	1630:1639	arg1	group					1649:1653	the equatorial hydroxy group	1626:1653	the equatorial hydroxy group at C4 of glucosides	1626:1673	Trp312 of the α-galactosidase appears to exclude the equatorial hydroxy group at C4 of glucosides, whereas the corresponding Trp residue in the α-glucoside hydrolase makes a hydrogen bond with this hydroxy group.
28103425	3	57	theme	hydrolase	574:582	arg1	α-galactosidase					599:613	a glycoside hydrolase family (GH) 97 α-galactosidase	562:613	a glycoside hydrolase family (GH) 97 α-galactosidase	562:613	Here, we investigate a transglycosylation catalyzed by a catalytic nucleophile mutant derived from a glycoside hydrolase family (GH) 97 α-galactosidase, using more stable β-galactosyl azide and α-galactosyl fluoride donors.
28103425	1	58	theme	nucleophile	182:192	arg1	mutant					194:199	a catalytic nucleophile mutant	170:199	a catalytic nucleophile mutant of a glycosidase	170:216	The preparation of a glycosynthase, a catalytic nucleophile mutant of a glycosidase, is a well-established strategy for the effective synthesis of glycosidic linkages.
28103425	1	58	theme	nucleophile	182:192	arg1	glycosynthase					155:167	a glycosynthase	153:167	a glycosynthase	153:167	The preparation of a glycosynthase, a catalytic nucleophile mutant of a glycosidase, is a well-established strategy for the effective synthesis of glycosidic linkages.
28103425	8	59	theme	β→α	1337:1339	arg1	loops					1341:1345	the β→α loops 1, 2 and 3	1333:1356	the β→α loops 1, 2 and 3 of the catalytic TIM barrel of the α-galactosidase from those of a homologous GH97 α-glucoside hydrolase	1333:1461	Subtle differences at the β→α loops 1, 2 and 3 of the catalytic TIM barrel of the α-galactosidase from those of a homologous GH97 α-glucoside hydrolase seem to be involved in substrate recognitions.
28103425	10	60	theme	glucosides	1664:1673	arg1	glucosides					1664:1673	glucosides	1664:1673	glucosides	1664:1673	Trp312 of the α-galactosidase appears to exclude the equatorial hydroxy group at C4 of glucosides, whereas the corresponding Trp residue in the α-glucoside hydrolase makes a hydrogen bond with this hydroxy group.
28103425	10	60	theme	glucosides	1664:1673	arg1	group					1649:1653	the equatorial hydroxy group	1626:1653	the equatorial hydroxy group at C4 of glucosides	1626:1673	Trp312 of the α-galactosidase appears to exclude the equatorial hydroxy group at C4 of glucosides, whereas the corresponding Trp residue in the α-glucoside hydrolase makes a hydrogen bond with this hydroxy group.
28103425	6	61	theme	complex	1052:1058	arg1	activity					1028:1035	low activity	1024:1035	low activity of the ternary complex between enzyme, β-galactosyl azide and acceptor	1024:1106	Kinetic analysis suggests that poor transglycosylation in the presence of the azide is because of low activity of the ternary complex between enzyme, β-galactosyl azide and acceptor.
28103425	7	62	theme	enzyme	1153:1158	arg1	structure					1129:1137	A three-dimensional structure	1109:1137	A three-dimensional structure of the mutant enzyme in complex with the transglycosylation product, β-lactosyl α-d-galactoside,	1109:1234	A three-dimensional structure of the mutant enzyme in complex with the transglycosylation product, β-lactosyl α-d-galactoside, was solved to elucidate the ligand-binding aspects of the α-galactosidase.
28103425	1	63	theme	glycosidase	206:216	arg1	mutant					194:199	a catalytic nucleophile mutant	170:199	a catalytic nucleophile mutant of a glycosidase	170:216	The preparation of a glycosynthase, a catalytic nucleophile mutant of a glycosidase, is a well-established strategy for the effective synthesis of glycosidic linkages.
28103425	1	63	theme	glycosidase	206:216	arg1	glycosynthase					155:167	a glycosynthase	153:167	a glycosynthase	153:167	The preparation of a glycosynthase, a catalytic nucleophile mutant of a glycosidase, is a well-established strategy for the effective synthesis of glycosidic linkages.
28103425	4	64	theme	β-galactosyl	748:759	arg1	azide					761:765	β-galactosyl azide	748:765	β-galactosyl azide	748:765	The mutant enzyme catalyzes the glycosynthase reaction using β-galactosyl azide and α-galactosyl transfer from α-galactosyl fluoride with assistance of external anions.
28103425	10	65	theme	corresponding	1688:1700	arg1	residue					1706:1712	the corresponding Trp residue	1684:1712	the corresponding Trp residue in the α-glucoside hydrolase	1684:1741	Trp312 of the α-galactosidase appears to exclude the equatorial hydroxy group at C4 of glucosides, whereas the corresponding Trp residue in the α-glucoside hydrolase makes a hydrogen bond with this hydroxy group.
28103425	3	66	theme	stable	627:632	arg1	azide					647:651	more stable β-galactosyl azide and α-galactosyl fluoride donors	622:684	azide	647:651	Here, we investigate a transglycosylation catalyzed by a catalytic nucleophile mutant derived from a glycoside hydrolase family (GH) 97 α-galactosidase, using more stable β-galactosyl azide and α-galactosyl fluoride donors.
28103425	9	67	theme	β→α	1545:1547	arg1	loop					1549:1552	β→α loop 1	1545:1554	β→α loop 1	1545:1554	In particular, the Trp residues in β→α loop 1 have separate roles.
28103425	8	68	theme	Subtle	1311:1316	arg1	differences					1318:1328	Subtle differences	1311:1328	Subtle differences at the β→α loops 1, 2 and 3 of the catalytic TIM barrel of the α-galactosidase from those of a homologous GH97 α-glucoside hydrolase	1311:1461	Subtle differences at the β→α loops 1, 2 and 3 of the catalytic TIM barrel of the α-galactosidase from those of a homologous GH97 α-glucoside hydrolase seem to be involved in substrate recognitions.
28103425	10	69	dep	appears	1607:1613	arg1	whereas					1676:1682	whereas	1676:1682	whereas	1676:1682	Trp312 of the α-galactosidase appears to exclude the equatorial hydroxy group at C4 of glucosides, whereas the corresponding Trp residue in the α-glucoside hydrolase makes a hydrogen bond with this hydroxy group.
28103425	3	70	theme	α-galactosyl	657:668	arg1	donors					679:684	more stable β-galactosyl azide and α-galactosyl fluoride donors	622:684	donors	679:684	Here, we investigate a transglycosylation catalyzed by a catalytic nucleophile mutant derived from a glycoside hydrolase family (GH) 97 α-galactosidase, using more stable β-galactosyl azide and α-galactosyl fluoride donors.
28103425	9	71	theme	separate	1561:1568	arg1	roles					1570:1574	separate roles	1561:1574	separate roles	1561:1574	In particular, the Trp residues in β→α loop 1 have separate roles.
28103425	3	72	attach	derived	549:555	arg1	α-galactosidase					599:613	a glycoside hydrolase family (GH) 97 α-galactosidase	562:613	a glycoside hydrolase family (GH) 97 α-galactosidase	562:613	Here, we investigate a transglycosylation catalyzed by a catalytic nucleophile mutant derived from a glycoside hydrolase family (GH) 97 α-galactosidase, using more stable β-galactosyl azide and α-galactosyl fluoride donors.
28103425	3	72	attach	derived	549:555	arg2	mutant					542:547	a catalytic nucleophile mutant	518:547	a catalytic nucleophile mutant derived from a glycoside hydrolase family (GH) 97 α-galactosidase, using more stable β-galactosyl azide and α-galactosyl fluoride donors	518:684	Here, we investigate a transglycosylation catalyzed by a catalytic nucleophile mutant derived from a glycoside hydrolase family (GH) 97 α-galactosidase, using more stable β-galactosyl azide and α-galactosyl fluoride donors.
28103425	8	73	theme	TIM	1375:1377	arg1	barrel					1379:1384	the catalytic TIM barrel	1361:1384	the catalytic TIM barrel of the α-galactosidase from those of a homologous GH97 α-glucoside hydrolase	1361:1461	Subtle differences at the β→α loops 1, 2 and 3 of the catalytic TIM barrel of the α-galactosidase from those of a homologous GH97 α-glucoside hydrolase seem to be involved in substrate recognitions.
28103425	6	74	gly	transglycosylation	962:979	arg1	presence					988:995	the presence	984:995	the presence of the azide	984:1008	Kinetic analysis suggests that poor transglycosylation in the presence of the azide is because of low activity of the ternary complex between enzyme, β-galactosyl azide and acceptor.
28103425	11	75	theme	recognition	1821:1831	arg1	mechanism					1794:1802	The mechanism	1790:1802	The mechanism of α-galactoside recognition	1790:1831	The mechanism of α-galactoside recognition is conserved among GH27, 31, 36 and 97 α-galactosidases.
28103425	1	76	theme	glycosynthase	155:167	arg1	preparation					138:148	The preparation	134:148	The preparation of a glycosynthase, a catalytic nucleophile mutant of a glycosidase,	134:217	The preparation of a glycosynthase, a catalytic nucleophile mutant of a glycosidase, is a well-established strategy for the effective synthesis of glycosidic linkages.
28103425	1	76	theme	glycosynthase	155:167	arg1	strategy					241:248	a well-established strategy	222:248	a well-established strategy for the effective synthesis of glycosidic linkages	222:299	The preparation of a glycosynthase, a catalytic nucleophile mutant of a glycosidase, is a well-established strategy for the effective synthesis of glycosidic linkages.
28103425	6	77	theme	β-galactosyl	1076:1087	arg1	azide					1089:1093	β-galactosyl azide	1076:1093	β-galactosyl azide	1076:1093	Kinetic analysis suggests that poor transglycosylation in the presence of the azide is because of low activity of the ternary complex between enzyme, β-galactosyl azide and acceptor.
28103425	8	78	theme	α-galactosidase	1393:1407	arg1	barrel					1379:1384	the catalytic TIM barrel	1361:1384	the catalytic TIM barrel of the α-galactosidase from those of a homologous GH97 α-glucoside hydrolase	1361:1461	Subtle differences at the β→α loops 1, 2 and 3 of the catalytic TIM barrel of the α-galactosidase from those of a homologous GH97 α-glucoside hydrolase seem to be involved in substrate recognitions.
28103425	7	79	theme	α-galactosidase	1294:1308	arg1	aspects					1279:1285	the ligand-binding aspects	1260:1285	the ligand-binding aspects of the α-galactosidase	1260:1308	A three-dimensional structure of the mutant enzyme in complex with the transglycosylation product, β-lactosyl α-d-galactoside, was solved to elucidate the ligand-binding aspects of the α-galactosidase.
28103425	12	80	theme	Data	1970:1973	arg1	Bank					1975:1978	the Protein Data Bank	1958:1978	the Protein Data Bank	1958:1978	DATABASE The atomic coordinates (code: 5E1Q) have been deposited in the Protein Data Bank.
28103425	3	81	theme	catalytic	520:528	arg1	mutant					542:547	a catalytic nucleophile mutant	518:547	a catalytic nucleophile mutant derived from a glycoside hydrolase family (GH) 97 α-galactosidase, using more stable β-galactosyl azide and α-galactosyl fluoride donors	518:684	Here, we investigate a transglycosylation catalyzed by a catalytic nucleophile mutant derived from a glycoside hydrolase family (GH) 97 α-galactosidase, using more stable β-galactosyl azide and α-galactosyl fluoride donors.
28103425	4	82	theme	anions	848:853	arg1	assistance					825:834	assistance	825:834	assistance of external anions	825:853	The mutant enzyme catalyzes the glycosynthase reaction using β-galactosyl azide and α-galactosyl transfer from α-galactosyl fluoride with assistance of external anions.
28103425	7	83	theme	ligand-binding	1264:1277	arg1	aspects					1279:1285	the ligand-binding aspects	1260:1285	the ligand-binding aspects of the α-galactosidase	1260:1308	A three-dimensional structure of the mutant enzyme in complex with the transglycosylation product, β-lactosyl α-d-galactoside, was solved to elucidate the ligand-binding aspects of the α-galactosidase.
28103425	7	84	from	complex	1163:1169	arg1	structure					1129:1137	A three-dimensional structure	1109:1137	A three-dimensional structure of the mutant enzyme in complex with the transglycosylation product, β-lactosyl α-d-galactoside,	1109:1234	A three-dimensional structure of the mutant enzyme in complex with the transglycosylation product, β-lactosyl α-d-galactoside, was solved to elucidate the ligand-binding aspects of the α-galactosidase.
28103425	4	85	theme	α-galactosyl	798:809	arg1	fluoride					811:818	α-galactosyl fluoride	798:818	α-galactosyl fluoride with assistance of external anions	798:853	The mutant enzyme catalyzes the glycosynthase reaction using β-galactosyl azide and α-galactosyl transfer from α-galactosyl fluoride with assistance of external anions.
28103425	10	86	theme	Trp	1702:1704	arg1	residue					1706:1712	the corresponding Trp residue	1684:1712	the corresponding Trp residue in the α-glucoside hydrolase	1684:1741	Trp312 of the α-galactosidase appears to exclude the equatorial hydroxy group at C4 of glucosides, whereas the corresponding Trp residue in the α-glucoside hydrolase makes a hydrogen bond with this hydroxy group.
28103425	0	87	theme	α-galactosyl	23:34	arg1	oligosaccharides					36:51	α-galactosyl oligosaccharides	23:51	α-galactosyl oligosaccharides using a mutant Bacteroides thetaiotaomicron	23:95	Efficient synthesis of α-galactosyl oligosaccharides using a mutant Bacteroides thetaiotaomicron retaining α-galactosidase (BtGH97b).
28103425	2	88	theme	β-glycosyl	435:444	arg1	donors					455:460	generally unstable β-glycosyl fluoride donors	416:460	generally unstable β-glycosyl fluoride donors	416:460	However, glycosynthases derived from α-glycosidases can give poor yields of desired products because they require generally unstable β-glycosyl fluoride donors.
28103425	0	89	theme	mutant	61:66	arg1	thetaiotaomicron					80:95	a mutant Bacteroides thetaiotaomicron	59:95	a mutant Bacteroides thetaiotaomicron	59:95	Efficient synthesis of α-galactosyl oligosaccharides using a mutant Bacteroides thetaiotaomicron retaining α-galactosidase (BtGH97b).
28103425	7	90	with	complex	1163:1169	arg1	product					1199:1205	the transglycosylation product	1176:1205	the transglycosylation product	1176:1205	A three-dimensional structure of the mutant enzyme in complex with the transglycosylation product, β-lactosyl α-d-galactoside, was solved to elucidate the ligand-binding aspects of the α-galactosidase.
28103425	7	90	with	complex	1163:1169	arg1	α-d-galactoside					1219:1233	β-lactosyl α-d-galactoside	1208:1233	β-lactosyl α-d-galactoside	1208:1233	A three-dimensional structure of the mutant enzyme in complex with the transglycosylation product, β-lactosyl α-d-galactoside, was solved to elucidate the ligand-binding aspects of the α-galactosidase.
28103425	2	91	theme	products	386:393	arg1	yields					368:373	poor yields	363:373	poor yields of desired products	363:393	However, glycosynthases derived from α-glycosidases can give poor yields of desired products because they require generally unstable β-glycosyl fluoride donors.
28103425	2	92	theme	poor	363:366	arg1	yields					368:373	poor yields	363:373	poor yields of desired products	363:393	However, glycosynthases derived from α-glycosidases can give poor yields of desired products because they require generally unstable β-glycosyl fluoride donors.
28103425	3	93	theme	β-galactosyl	634:645	arg1	azide					647:651	more stable β-galactosyl azide and α-galactosyl fluoride donors	622:684	azide	647:651	Here, we investigate a transglycosylation catalyzed by a catalytic nucleophile mutant derived from a glycoside hydrolase family (GH) 97 α-galactosidase, using more stable β-galactosyl azide and α-galactosyl fluoride donors.
28103425	8	94	theme	substrate	1486:1494	arg1	recognitions					1496:1507	substrate recognitions	1486:1507	substrate recognitions	1486:1507	Subtle differences at the β→α loops 1, 2 and 3 of the catalytic TIM barrel of the α-galactosidase from those of a homologous GH97 α-glucoside hydrolase seem to be involved in substrate recognitions.
28103425	10	95	theme	hydrogen	1751:1758	arg1	bond					1760:1763	a hydrogen bond	1749:1763	a hydrogen bond with this hydroxy group	1749:1787	Trp312 of the α-galactosidase appears to exclude the equatorial hydroxy group at C4 of glucosides, whereas the corresponding Trp residue in the α-glucoside hydrolase makes a hydrogen bond with this hydroxy group.
28103425	7	96	from	structure	1129:1137	arg1	complex					1163:1169	complex	1163:1169	complex with the transglycosylation product, β-lactosyl α-d-galactoside,	1163:1234	A three-dimensional structure of the mutant enzyme in complex with the transglycosylation product, β-lactosyl α-d-galactoside, was solved to elucidate the ligand-binding aspects of the α-galactosidase.
28103425	3	97	theme	glycoside	564:572	arg1	α-galactosidase					599:613	a glycoside hydrolase family (GH) 97 α-galactosidase	562:613	a glycoside hydrolase family (GH) 97 α-galactosidase	562:613	Here, we investigate a transglycosylation catalyzed by a catalytic nucleophile mutant derived from a glycoside hydrolase family (GH) 97 α-galactosidase, using more stable β-galactosyl azide and α-galactosyl fluoride donors.
24351858	6	0	from	factors	862:868	arg1	growth					887:892	the bacterial growth	873:892	the bacterial growth	873:892	Estimated optimum conditions of the factors on the bacterial growth are as follows: lactose concentration=9·5 g/l; inulin concentration=38·5 mg/l; yeast extract concentration=9·6 g/l and initial pH=6·2.
24351858	1	1	theme	probiotic	236:244	arg1	bacterium					246:254	a probiotic bacterium	234:254	a probiotic bacterium	234:254	This study was aimed to optimise lactose, inulin and yeast extract concentration and also culture pH for maximising the growth of a probiotic bacterium, Bifidobacterium animalis subsp.
24351858	0	2	theme	surface	83:89	arg1	methodology					91:101	response surface methodology	74:101	response surface methodology	74:101	Optimisation of medium composition for probiotic biomass production using response surface methodology.
24351858	6	3	theme	bacterial	877:885	arg1	growth					887:892	the bacterial growth	873:892	the bacterial growth	873:892	Estimated optimum conditions of the factors on the bacterial growth are as follows: lactose concentration=9·5 g/l; inulin concentration=38·5 mg/l; yeast extract concentration=9·6 g/l and initial pH=6·2.
24351858	1	4	theme	yeast	157:161	arg1	extract					163:169	yeast extract	157:169	yeast extract	157:169	This study was aimed to optimise lactose, inulin and yeast extract concentration and also culture pH for maximising the growth of a probiotic bacterium, Bifidobacterium animalis subsp.
24351858	1	5	theme	bacterium	246:254	arg1	growth					224:229	the growth	220:229	the growth of a probiotic bacterium, Bifidobacterium animalis subsp	220:286	This study was aimed to optimise lactose, inulin and yeast extract concentration and also culture pH for maximising the growth of a probiotic bacterium, Bifidobacterium animalis subsp.
24351858	1	6	dep	subsp	282:286	arg1	Bifidobacterium					257:271	Bifidobacterium	257:271	Bifidobacterium	257:271	This study was aimed to optimise lactose, inulin and yeast extract concentration and also culture pH for maximising the growth of a probiotic bacterium, Bifidobacterium animalis subsp.
24351858	6	7	theme	yeast	973:977	arg1	concentration=9·6 g/l					987:1007	yeast extract concentration=9·6 g/l	973:1007	yeast extract concentration=9·6 g/l	973:1007	Estimated optimum conditions of the factors on the bacterial growth are as follows: lactose concentration=9·5 g/l; inulin concentration=38·5 mg/l; yeast extract concentration=9·6 g/l and initial pH=6·2.
24351858	5	8	theme	yeast	689:693	arg1	extract					695:701	yeast extract	689:701	yeast extract	689:701	It was found that the effects involving inulin, yeast extract and pH on growth of the bacterium were significant, and the strongest effect was given by the yeast extract concentration.
24351858	6	9	theme	factors	862:868	arg1	conditions					844:853	Estimated optimum conditions	826:853	Estimated optimum conditions of the factors on the bacterial growth	826:892	Estimated optimum conditions of the factors on the bacterial growth are as follows: lactose concentration=9·5 g/l; inulin concentration=38·5 mg/l; yeast extract concentration=9·6 g/l and initial pH=6·2.
24351858	3	10	theme	microorganism	517:529	arg1	growth					503:508	growth	503:508	growth of the microorganism	503:529	A second-order central composite design was applied to evaluate the effects of these independent variables on growth of the microorganism.
24351858	2	11	theme	response	363:370	arg1	methodology					380:390	response surface methodology	363:390	response surface methodology	363:390	lactis in apple juice and to assess the effects of these factors by using response surface methodology.
24351858	1	12	theme	extract	163:169	arg1	concentration					171:183	lactose, inulin and yeast extract concentration	137:183	lactose, inulin and yeast extract concentration	137:183	This study was aimed to optimise lactose, inulin and yeast extract concentration and also culture pH for maximising the growth of a probiotic bacterium, Bifidobacterium animalis subsp.
24351858	5	13	theme	strongest	763:771	arg1	effect					773:778	the strongest effect	759:778	the strongest effect	759:778	It was found that the effects involving inulin, yeast extract and pH on growth of the bacterium were significant, and the strongest effect was given by the yeast extract concentration.
24351858	3	14	theme	second-order	395:406	arg1	design					426:431	A second-order central composite design	393:431	A second-order central composite design	393:431	A second-order central composite design was applied to evaluate the effects of these independent variables on growth of the microorganism.
24351858	2	15	theme	surface	372:378	arg1	methodology					380:390	response surface methodology	363:390	response surface methodology	363:390	lactis in apple juice and to assess the effects of these factors by using response surface methodology.
24351858	4	16	theme	quadratic	577:585	arg1	terms					587:591	cubic and quadratic terms	567:591	cubic and quadratic terms	567:591	A polynomial regression model with cubic and quadratic terms was used for analysis of the experimental data.
24351858	6	17	from	growth	887:892	arg1	conditions					844:853	Estimated optimum conditions	826:853	Estimated optimum conditions of the factors on the bacterial growth	826:892	Estimated optimum conditions of the factors on the bacterial growth are as follows: lactose concentration=9·5 g/l; inulin concentration=38·5 mg/l; yeast extract concentration=9·6 g/l and initial pH=6·2.
24351858	3	18	theme	composite	416:424	arg1	design					426:431	A second-order central composite design	393:431	A second-order central composite design	393:431	A second-order central composite design was applied to evaluate the effects of these independent variables on growth of the microorganism.
24351858	1	19	theme	animalis	273:280	arg1	subsp					282:286	animalis subsp	273:286	animalis subsp	273:286	This study was aimed to optimise lactose, inulin and yeast extract concentration and also culture pH for maximising the growth of a probiotic bacterium, Bifidobacterium animalis subsp.
24351858	0	20	theme	composition	23:33	arg1	Optimisation					0:11	Optimisation	0:11	Optimisation of medium composition for probiotic biomass production	0:66	Optimisation of medium composition for probiotic biomass production using response surface methodology.
24351858	4	21	theme	regression	545:554	arg1	model					556:560	A polynomial regression model	532:560	A polynomial regression model with cubic and quadratic terms	532:591	A polynomial regression model with cubic and quadratic terms was used for analysis of the experimental data.
24351858	2	22	theme	apple	299:303	arg1	juice					305:309	apple juice	299:309	apple juice	299:309	lactis in apple juice and to assess the effects of these factors by using response surface methodology.
24351858	1	23	theme	culture	194:200	arg1	pH					202:203	culture pH	194:203	culture pH for maximising the growth of a probiotic bacterium, Bifidobacterium animalis subsp	194:286	This study was aimed to optimise lactose, inulin and yeast extract concentration and also culture pH for maximising the growth of a probiotic bacterium, Bifidobacterium animalis subsp.
24351858	0	24	theme	medium	16:21	arg1	composition					23:33	medium composition	16:33	medium composition	16:33	Optimisation of medium composition for probiotic biomass production using response surface methodology.
24351858	4	25	theme	cubic	567:571	arg1	terms					587:591	cubic and quadratic terms	567:591	cubic and quadratic terms	567:591	A polynomial regression model with cubic and quadratic terms was used for analysis of the experimental data.
24351858	4	26	with	model	556:560	arg1	terms					587:591	cubic and quadratic terms	567:591	cubic and quadratic terms	567:591	A polynomial regression model with cubic and quadratic terms was used for analysis of the experimental data.
24351858	6	27	dep	follows	901:907	arg1	concentration=9·6 g/l					987:1007	yeast extract concentration=9·6 g/l	973:1007	yeast extract concentration=9·6 g/l	973:1007	Estimated optimum conditions of the factors on the bacterial growth are as follows: lactose concentration=9·5 g/l; inulin concentration=38·5 mg/l; yeast extract concentration=9·6 g/l and initial pH=6·2.
24351858	6	27	dep	follows	901:907	arg1	pH=6·2					1021:1026	initial pH=6·2	1013:1026	initial pH=6·2	1013:1026	Estimated optimum conditions of the factors on the bacterial growth are as follows: lactose concentration=9·5 g/l; inulin concentration=38·5 mg/l; yeast extract concentration=9·6 g/l and initial pH=6·2.
24351858	6	27	dep	follows	901:907	arg1	concentration=9·5 g/l					918:938	lactose concentration=9·5 g/l	910:938	follows: lactose concentration=9·5 g/l; inulin concentration=38·5 mg/l; yeast extract concentration=9·6 g/l and initial pH=6·2	901:1026	Estimated optimum conditions of the factors on the bacterial growth are as follows: lactose concentration=9·5 g/l; inulin concentration=38·5 mg/l; yeast extract concentration=9·6 g/l and initial pH=6·2.
24351858	6	27	dep	follows	901:907	arg1	concentration=38·5 mg/l					948:970	inulin concentration=38·5 mg/l	941:970	follows: lactose concentration=9·5 g/l; inulin concentration=38·5 mg/l; yeast extract concentration=9·6 g/l and initial pH=6·2	901:1026	Estimated optimum conditions of the factors on the bacterial growth are as follows: lactose concentration=9·5 g/l; inulin concentration=38·5 mg/l; yeast extract concentration=9·6 g/l and initial pH=6·2.
24351858	5	28	theme	yeast	797:801	arg1	concentration					811:823	the yeast extract concentration	793:823	the yeast extract concentration	793:823	It was found that the effects involving inulin, yeast extract and pH on growth of the bacterium were significant, and the strongest effect was given by the yeast extract concentration.
24351858	0	29	theme	probiotic	39:47	arg1	production					57:66	probiotic biomass production	39:66	probiotic biomass production	39:66	Optimisation of medium composition for probiotic biomass production using response surface methodology.
24351858	4	30	used	used	597:600	arg2	model					556:560	A polynomial regression model	532:560	A polynomial regression model with cubic and quadratic terms	532:591	A polynomial regression model with cubic and quadratic terms was used for analysis of the experimental data.
24351858	6	31	theme	optimum	836:842	arg1	conditions					844:853	Estimated optimum conditions	826:853	Estimated optimum conditions of the factors on the bacterial growth	826:892	Estimated optimum conditions of the factors on the bacterial growth are as follows: lactose concentration=9·5 g/l; inulin concentration=38·5 mg/l; yeast extract concentration=9·6 g/l and initial pH=6·2.
24351858	5	32	theme	extract	803:809	arg1	concentration					811:823	the yeast extract concentration	793:823	the yeast extract concentration	793:823	It was found that the effects involving inulin, yeast extract and pH on growth of the bacterium were significant, and the strongest effect was given by the yeast extract concentration.
24351858	3	33	theme	central	408:414	arg1	design					426:431	A second-order central composite design	393:431	A second-order central composite design	393:431	A second-order central composite design was applied to evaluate the effects of these independent variables on growth of the microorganism.
24351858	6	34	theme	lactose	910:916	arg1	concentration=9·5 g/l					918:938	lactose concentration=9·5 g/l	910:938	follows: lactose concentration=9·5 g/l; inulin concentration=38·5 mg/l; yeast extract concentration=9·6 g/l and initial pH=6·2	901:1026	Estimated optimum conditions of the factors on the bacterial growth are as follows: lactose concentration=9·5 g/l; inulin concentration=38·5 mg/l; yeast extract concentration=9·6 g/l and initial pH=6·2.
24351858	6	35	theme	extract	979:985	arg1	concentration=9·6 g/l					987:1007	yeast extract concentration=9·6 g/l	973:1007	yeast extract concentration=9·6 g/l	973:1007	Estimated optimum conditions of the factors on the bacterial growth are as follows: lactose concentration=9·5 g/l; inulin concentration=38·5 mg/l; yeast extract concentration=9·6 g/l and initial pH=6·2.
24351858	6	36	theme	Estimated	826:834	arg1	conditions					844:853	Estimated optimum conditions	826:853	Estimated optimum conditions of the factors on the bacterial growth	826:892	Estimated optimum conditions of the factors on the bacterial growth are as follows: lactose concentration=9·5 g/l; inulin concentration=38·5 mg/l; yeast extract concentration=9·6 g/l and initial pH=6·2.
24351858	6	37	from	conditions	844:853	arg1	growth					887:892	the bacterial growth	873:892	the bacterial growth	873:892	Estimated optimum conditions of the factors on the bacterial growth are as follows: lactose concentration=9·5 g/l; inulin concentration=38·5 mg/l; yeast extract concentration=9·6 g/l and initial pH=6·2.
24351858	4	38	theme	data	635:638	arg1	analysis					606:613	analysis	606:613	analysis of the experimental data	606:638	A polynomial regression model with cubic and quadratic terms was used for analysis of the experimental data.
24351858	6	39	theme	inulin	941:946	arg1	concentration=38·5 mg/l					948:970	inulin concentration=38·5 mg/l	941:970	follows: lactose concentration=9·5 g/l; inulin concentration=38·5 mg/l; yeast extract concentration=9·6 g/l and initial pH=6·2	901:1026	Estimated optimum conditions of the factors on the bacterial growth are as follows: lactose concentration=9·5 g/l; inulin concentration=38·5 mg/l; yeast extract concentration=9·6 g/l and initial pH=6·2.
24351858	3	40	theme	independent	478:488	arg1	variables					490:498	these independent variables	472:498	these independent variables	472:498	A second-order central composite design was applied to evaluate the effects of these independent variables on growth of the microorganism.
24351858	0	41	theme	biomass	49:55	arg1	production					57:66	probiotic biomass production	39:66	probiotic biomass production	39:66	Optimisation of medium composition for probiotic biomass production using response surface methodology.
24351858	4	42	theme	polynomial	534:543	arg1	model					556:560	A polynomial regression model	532:560	A polynomial regression model with cubic and quadratic terms	532:591	A polynomial regression model with cubic and quadratic terms was used for analysis of the experimental data.
24351858	1	43	theme	inulin	146:151	arg1	concentration					171:183	lactose, inulin and yeast extract concentration	137:183	lactose, inulin and yeast extract concentration	137:183	This study was aimed to optimise lactose, inulin and yeast extract concentration and also culture pH for maximising the growth of a probiotic bacterium, Bifidobacterium animalis subsp.
24351858	3	44	theme	variables	490:498	arg1	effects					461:467	the effects	457:467	the effects of these independent variables on growth of the microorganism	457:529	A second-order central composite design was applied to evaluate the effects of these independent variables on growth of the microorganism.
24351858	3	45	from	effects	461:467	arg1	growth					503:508	growth	503:508	growth of the microorganism	503:529	A second-order central composite design was applied to evaluate the effects of these independent variables on growth of the microorganism.
24351858	1	46	theme	lactose	137:143	arg1	concentration					171:183	lactose, inulin and yeast extract concentration	137:183	lactose, inulin and yeast extract concentration	137:183	This study was aimed to optimise lactose, inulin and yeast extract concentration and also culture pH for maximising the growth of a probiotic bacterium, Bifidobacterium animalis subsp.
24351858	2	47	from	lactis	289:294	arg1	juice					305:309	apple juice	299:309	apple juice	299:309	lactis in apple juice and to assess the effects of these factors by using response surface methodology.
24351858	0	48	theme	response	74:81	arg1	methodology					91:101	response surface methodology	74:101	response surface methodology	74:101	Optimisation of medium composition for probiotic biomass production using response surface methodology.
24351858	4	49	theme	experimental	622:633	arg1	data					635:638	the experimental data	618:638	the experimental data	618:638	A polynomial regression model with cubic and quadratic terms was used for analysis of the experimental data.
24351858	6	50	theme	initial	1013:1019	arg1	pH=6·2					1021:1026	initial pH=6·2	1013:1026	initial pH=6·2	1013:1026	Estimated optimum conditions of the factors on the bacterial growth are as follows: lactose concentration=9·5 g/l; inulin concentration=38·5 mg/l; yeast extract concentration=9·6 g/l and initial pH=6·2.
24351858	5	51	theme	bacterium	727:735	arg1	growth					713:718	growth	713:718	growth of the bacterium	713:735	It was found that the effects involving inulin, yeast extract and pH on growth of the bacterium were significant, and the strongest effect was given by the yeast extract concentration.
24351858	2	52	theme	factors	346:352	arg1	effects					329:335	the effects	325:335	the effects of these factors	325:352	lactis in apple juice and to assess the effects of these factors by using response surface methodology.
24351858	5	53	from	effects	663:669	arg1	growth					713:718	growth	713:718	growth of the bacterium	713:735	It was found that the effects involving inulin, yeast extract and pH on growth of the bacterium were significant, and the strongest effect was given by the yeast extract concentration.
28385225	6	0	theme	high	923:926	arg1	8mgBL-1					945:951	8mgBL-1	945:951	8mgBL-1	945:951	Even at the high B concentration (8mgBL-1), B in drinking water could be reduced to less than 2.4mgL -1 when 0.05g plant-based chitosan composite beads and 12 Lemna fronds were used for 50mL test solution.
28385225	6	0	theme	high	923:926	arg1	concentration					930:942	the high B concentration	919:942	the high B concentration (8mgBL-1)	919:952	Even at the high B concentration (8mgBL-1), B in drinking water could be reduced to less than 2.4mgL -1 when 0.05g plant-based chitosan composite beads and 12 Lemna fronds were used for 50mL test solution.
28385225	2	1	theme	effective	326:334	arg1	techniques					346:355	alternative and cost effective treatment techniques	305:355	alternative and cost effective treatment techniques	305:355	Clearly, alternative and cost effective treatment techniques are imperative.
28385225	0	2	theme	chitosan	68:75	arg1	composites					77:86	various chitosan composites	60:86	various chitosan composites	60:86	Evaluation and application of an innovative method based on various chitosan composites and Lemna gibba for boron removal from drinking water.
28385225	6	3	from	B	955:955	arg1	water					969:973	drinking water	960:973	drinking water	960:973	Even at the high B concentration (8mgBL-1), B in drinking water could be reduced to less than 2.4mgL -1 when 0.05g plant-based chitosan composite beads and 12 Lemna fronds were used for 50mL test solution.
28385225	3	4	theme	chitosan	487:494	arg1	beads					506:510	various chitosan composite beads	479:510	various chitosan composite beads	479:510	In the present study, an innovative and environment friendly method based on hybrid systems consisting of various chitosan composite beads and Lemna gibba were evaluated for removal of B from drinking water.
28385225	0	5	theme	Lemna	92:96	arg1	gibba					98:102	Lemna gibba	92:102	Lemna gibba for boron removal from drinking water	92:140	Evaluation and application of an innovative method based on various chitosan composites and Lemna gibba for boron removal from drinking water.
28385225	6	6	theme	Lemna	1070:1074	arg1	fronds					1076:1081	12 Lemna fronds	1067:1081	12 Lemna fronds	1067:1081	Even at the high B concentration (8mgBL-1), B in drinking water could be reduced to less than 2.4mgL -1 when 0.05g plant-based chitosan composite beads and 12 Lemna fronds were used for 50mL test solution.
28385225	4	7	theme	composite	742:750	arg1	beads					752:756	mineral-based chitosan composite beads	719:756	mineral-based chitosan composite beads	719:756	Our results from batch adsorption experiment indicated that a plant-based chitosan composite bead has a higher capacity of B removal than mineral-based chitosan composite beads.
28385225	3	8	theme	various	479:485	arg1	beads					506:510	various chitosan composite beads	479:510	various chitosan composite beads	479:510	In the present study, an innovative and environment friendly method based on hybrid systems consisting of various chitosan composite beads and Lemna gibba were evaluated for removal of B from drinking water.
28385225	0	9	from	water	136:140	arg1	removal					114:120	boron removal	108:120	boron removal from drinking water	108:140	Evaluation and application of an innovative method based on various chitosan composites and Lemna gibba for boron removal from drinking water.
28385225	6	10	theme	0.05g	1020:1024	arg1	beads					1057:1061	0.05g plant-based chitosan composite beads	1020:1061	0.05g plant-based chitosan composite beads	1020:1061	Even at the high B concentration (8mgBL-1), B in drinking water could be reduced to less than 2.4mgL -1 when 0.05g plant-based chitosan composite beads and 12 Lemna fronds were used for 50mL test solution.
28385225	4	11	theme	batch	598:602	arg1	experiment					615:624	batch adsorption experiment	598:624	batch adsorption experiment	598:624	Our results from batch adsorption experiment indicated that a plant-based chitosan composite bead has a higher capacity of B removal than mineral-based chitosan composite beads.
28385225	6	12	theme	50mL	1097:1100	arg1	solution					1107:1114	50mL test solution	1097:1114	50mL test solution	1097:1114	Even at the high B concentration (8mgBL-1), B in drinking water could be reduced to less than 2.4mgL -1 when 0.05g plant-based chitosan composite beads and 12 Lemna fronds were used for 50mL test solution.
28385225	5	13	theme	dried	835:839	arg1	beads					866:870	dried Lemna-chitosan composite beads	835:870	dried Lemna-chitosan composite beads	835:870	Almost 50% of total B removal was achieved using the hybrid system based on dried Lemna-chitosan composite beads and Lemna gibba combination in 4 days.
28385225	3	14	theme	present	380:386	arg1	study					388:392	the present study	376:392	the present study	376:392	In the present study, an innovative and environment friendly method based on hybrid systems consisting of various chitosan composite beads and Lemna gibba were evaluated for removal of B from drinking water.
28385225	5	15	theme	Lemna	876:880	arg1	combination					888:898	Lemna gibba combination	876:898	Lemna gibba combination	876:898	Almost 50% of total B removal was achieved using the hybrid system based on dried Lemna-chitosan composite beads and Lemna gibba combination in 4 days.
28385225	3	16	theme	Lemna	516:520	arg1	gibba					522:526	Lemna gibba	516:526	Lemna gibba	516:526	In the present study, an innovative and environment friendly method based on hybrid systems consisting of various chitosan composite beads and Lemna gibba were evaluated for removal of B from drinking water.
28385225	1	17	theme	various	159:165	arg1	types					167:171	various types	159:171	various types of water environments	159:193	Boron exists in various types of water environments, and it is difficult and costly to remove B with conventional treatment methods from drinking water.
28385225	5	18	theme	B	779:779	arg1	removal					781:787	total B removal	773:787	total B removal	773:787	Almost 50% of total B removal was achieved using the hybrid system based on dried Lemna-chitosan composite beads and Lemna gibba combination in 4 days.
28385225	6	19	used	used	1088:1091	arg2	beads					1057:1061	0.05g plant-based chitosan composite beads	1020:1061	0.05g plant-based chitosan composite beads	1020:1061	Even at the high B concentration (8mgBL-1), B in drinking water could be reduced to less than 2.4mgL -1 when 0.05g plant-based chitosan composite beads and 12 Lemna fronds were used for 50mL test solution.
28385225	6	19	used	used	1088:1091	arg2	fronds					1076:1081	12 Lemna fronds	1067:1081	12 Lemna fronds	1067:1081	Even at the high B concentration (8mgBL-1), B in drinking water could be reduced to less than 2.4mgL -1 when 0.05g plant-based chitosan composite beads and 12 Lemna fronds were used for 50mL test solution.
28385225	4	20	theme	chitosan	655:662	arg1	bead					674:677	a plant-based chitosan composite bead	641:677	a plant-based chitosan composite bead	641:677	Our results from batch adsorption experiment indicated that a plant-based chitosan composite bead has a higher capacity of B removal than mineral-based chitosan composite beads.
28385225	5	21	theme	removal	781:787	arg1	removal					781:787	total B removal	773:787	total B removal	773:787	Almost 50% of total B removal was achieved using the hybrid system based on dried Lemna-chitosan composite beads and Lemna gibba combination in 4 days.
28385225	5	21	theme	removal	781:787	arg1	%					768:768	Almost 50%	759:768	Almost 50% of total B removal	759:787	Almost 50% of total B removal was achieved using the hybrid system based on dried Lemna-chitosan composite beads and Lemna gibba combination in 4 days.
28385225	4	22	theme	removal	706:712	arg1	capacity					692:699	a higher capacity	683:699	a higher capacity of B removal	683:712	Our results from batch adsorption experiment indicated that a plant-based chitosan composite bead has a higher capacity of B removal than mineral-based chitosan composite beads.
28385225	4	23	theme	plant-based	643:653	arg1	bead					674:677	a plant-based chitosan composite bead	641:677	a plant-based chitosan composite bead	641:677	Our results from batch adsorption experiment indicated that a plant-based chitosan composite bead has a higher capacity of B removal than mineral-based chitosan composite beads.
28385225	1	24	theme	conventional	244:255	arg1	methods					267:273	conventional treatment methods	244:273	conventional treatment methods	244:273	Boron exists in various types of water environments, and it is difficult and costly to remove B with conventional treatment methods from drinking water.
28385225	5	25	from	beads	866:870	arg1	days					905:908	4 days	903:908	4 days	903:908	Almost 50% of total B removal was achieved using the hybrid system based on dried Lemna-chitosan composite beads and Lemna gibba combination in 4 days.
28385225	4	26	theme	chitosan	733:740	arg1	beads					752:756	mineral-based chitosan composite beads	719:756	mineral-based chitosan composite beads	719:756	Our results from batch adsorption experiment indicated that a plant-based chitosan composite bead has a higher capacity of B removal than mineral-based chitosan composite beads.
28385225	4	27	from	experiment	615:624	arg1	results					585:591	Our results	581:591	Our results from batch adsorption experiment	581:624	Our results from batch adsorption experiment indicated that a plant-based chitosan composite bead has a higher capacity of B removal than mineral-based chitosan composite beads.
28385225	1	28	theme	water	176:180	arg1	environments					182:193	water environments	176:193	water environments	176:193	Boron exists in various types of water environments, and it is difficult and costly to remove B with conventional treatment methods from drinking water.
28385225	6	29	theme	composite	1047:1055	arg1	beads					1057:1061	0.05g plant-based chitosan composite beads	1020:1061	0.05g plant-based chitosan composite beads	1020:1061	Even at the high B concentration (8mgBL-1), B in drinking water could be reduced to less than 2.4mgL -1 when 0.05g plant-based chitosan composite beads and 12 Lemna fronds were used for 50mL test solution.
28385225	1	30	theme	treatment	257:265	arg1	methods					267:273	conventional treatment methods	244:273	conventional treatment methods	244:273	Boron exists in various types of water environments, and it is difficult and costly to remove B with conventional treatment methods from drinking water.
28385225	0	31	theme	boron	108:112	arg1	removal					114:120	boron removal	108:120	boron removal from drinking water	108:140	Evaluation and application of an innovative method based on various chitosan composites and Lemna gibba for boron removal from drinking water.
28385225	4	32	theme	mineral-based	719:731	arg1	beads					752:756	mineral-based chitosan composite beads	719:756	mineral-based chitosan composite beads	719:756	Our results from batch adsorption experiment indicated that a plant-based chitosan composite bead has a higher capacity of B removal than mineral-based chitosan composite beads.
28385225	1	33	theme	environments	182:193	arg1	types					167:171	various types	159:171	various types of water environments	159:193	Boron exists in various types of water environments, and it is difficult and costly to remove B with conventional treatment methods from drinking water.
28385225	0	34	theme	innovative	33:42	arg1	method					44:49	an innovative method	30:49	an innovative method	30:49	Evaluation and application of an innovative method based on various chitosan composites and Lemna gibba for boron removal from drinking water.
28385225	4	35	theme	higher	685:690	arg1	capacity					692:699	a higher capacity	683:699	a higher capacity of B removal	683:712	Our results from batch adsorption experiment indicated that a plant-based chitosan composite bead has a higher capacity of B removal than mineral-based chitosan composite beads.
28385225	0	36	theme	drinking	127:134	arg1	water					136:140	drinking water	127:140	drinking water	127:140	Evaluation and application of an innovative method based on various chitosan composites and Lemna gibba for boron removal from drinking water.
28385225	5	37	theme	hybrid	812:817	arg1	system					819:824	the hybrid system	808:824	the hybrid system based on dried Lemna-chitosan composite beads and Lemna gibba combination in 4 days	808:908	Almost 50% of total B removal was achieved using the hybrid system based on dried Lemna-chitosan composite beads and Lemna gibba combination in 4 days.
28385225	3	38	theme	drinking	565:572	arg1	water					574:578	drinking water	565:578	drinking water	565:578	In the present study, an innovative and environment friendly method based on hybrid systems consisting of various chitosan composite beads and Lemna gibba were evaluated for removal of B from drinking water.
28385225	3	39	from	water	574:578	arg1	removal					547:553	removal	547:553	removal of B from drinking water	547:578	In the present study, an innovative and environment friendly method based on hybrid systems consisting of various chitosan composite beads and Lemna gibba were evaluated for removal of B from drinking water.
28385225	3	40	theme	friendly	425:432	arg1	method					434:439	an innovative and environment friendly method	395:439	method	434:439	In the present study, an innovative and environment friendly method based on hybrid systems consisting of various chitosan composite beads and Lemna gibba were evaluated for removal of B from drinking water.
28385225	5	41	theme	composite	856:864	arg1	beads					866:870	dried Lemna-chitosan composite beads	835:870	dried Lemna-chitosan composite beads	835:870	Almost 50% of total B removal was achieved using the hybrid system based on dried Lemna-chitosan composite beads and Lemna gibba combination in 4 days.
28385225	5	42	theme	total	773:777	arg1	removal					781:787	total B removal	773:787	total B removal	773:787	Almost 50% of total B removal was achieved using the hybrid system based on dried Lemna-chitosan composite beads and Lemna gibba combination in 4 days.
28385225	6	43	theme	B	928:928	arg1	8mgBL-1					945:951	8mgBL-1	945:951	8mgBL-1	945:951	Even at the high B concentration (8mgBL-1), B in drinking water could be reduced to less than 2.4mgL -1 when 0.05g plant-based chitosan composite beads and 12 Lemna fronds were used for 50mL test solution.
28385225	6	43	theme	B	928:928	arg1	concentration					930:942	the high B concentration	919:942	the high B concentration (8mgBL-1)	919:952	Even at the high B concentration (8mgBL-1), B in drinking water could be reduced to less than 2.4mgL -1 when 0.05g plant-based chitosan composite beads and 12 Lemna fronds were used for 50mL test solution.
28385225	3	44	theme	B	558:558	arg1	removal					547:553	removal	547:553	removal of B from drinking water	547:578	In the present study, an innovative and environment friendly method based on hybrid systems consisting of various chitosan composite beads and Lemna gibba were evaluated for removal of B from drinking water.
28385225	6	45	theme	chitosan	1038:1045	arg1	beads					1057:1061	0.05g plant-based chitosan composite beads	1020:1061	0.05g plant-based chitosan composite beads	1020:1061	Even at the high B concentration (8mgBL-1), B in drinking water could be reduced to less than 2.4mgL -1 when 0.05g plant-based chitosan composite beads and 12 Lemna fronds were used for 50mL test solution.
28385225	5	46	theme	gibba	882:886	arg1	combination					888:898	Lemna gibba combination	876:898	Lemna gibba combination	876:898	Almost 50% of total B removal was achieved using the hybrid system based on dried Lemna-chitosan composite beads and Lemna gibba combination in 4 days.
28385225	0	47	theme	method	44:49	arg1	application					15:25	application	15:25	application	15:25	Evaluation and application of an innovative method based on various chitosan composites and Lemna gibba for boron removal from drinking water.
28385225	0	47	theme	method	44:49	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation	0:9	Evaluation and application of an innovative method based on various chitosan composites and Lemna gibba for boron removal from drinking water.
28385225	6	48	theme	test	1102:1105	arg1	solution					1107:1114	50mL test solution	1097:1114	50mL test solution	1097:1114	Even at the high B concentration (8mgBL-1), B in drinking water could be reduced to less than 2.4mgL -1 when 0.05g plant-based chitosan composite beads and 12 Lemna fronds were used for 50mL test solution.
28385225	2	49	theme	alternative	305:315	arg1	techniques					346:355	alternative and cost effective treatment techniques	305:355	alternative and cost effective treatment techniques	305:355	Clearly, alternative and cost effective treatment techniques are imperative.
28385225	5	50	from	combination	888:898	arg1	days					905:908	4 days	903:908	4 days	903:908	Almost 50% of total B removal was achieved using the hybrid system based on dried Lemna-chitosan composite beads and Lemna gibba combination in 4 days.
28385225	6	51	theme	drinking	960:967	arg1	water					969:973	drinking water	960:973	drinking water	960:973	Even at the high B concentration (8mgBL-1), B in drinking water could be reduced to less than 2.4mgL -1 when 0.05g plant-based chitosan composite beads and 12 Lemna fronds were used for 50mL test solution.
28385225	3	52	theme	hybrid	450:455	arg1	systems					457:463	hybrid systems	450:463	hybrid systems consisting of various chitosan composite beads and Lemna gibba	450:526	In the present study, an innovative and environment friendly method based on hybrid systems consisting of various chitosan composite beads and Lemna gibba were evaluated for removal of B from drinking water.
28385225	5	53	theme	Lemna-chitosan	841:854	arg1	beads					866:870	dried Lemna-chitosan composite beads	835:870	dried Lemna-chitosan composite beads	835:870	Almost 50% of total B removal was achieved using the hybrid system based on dried Lemna-chitosan composite beads and Lemna gibba combination in 4 days.
28385225	4	54	theme	composite	664:672	arg1	bead					674:677	a plant-based chitosan composite bead	641:677	a plant-based chitosan composite bead	641:677	Our results from batch adsorption experiment indicated that a plant-based chitosan composite bead has a higher capacity of B removal than mineral-based chitosan composite beads.
28385225	4	55	theme	B	704:704	arg1	removal					706:712	B removal	704:712	B removal	704:712	Our results from batch adsorption experiment indicated that a plant-based chitosan composite bead has a higher capacity of B removal than mineral-based chitosan composite beads.
28385225	0	56	theme	various	60:66	arg1	composites					77:86	various chitosan composites	60:86	various chitosan composites	60:86	Evaluation and application of an innovative method based on various chitosan composites and Lemna gibba for boron removal from drinking water.
28385225	6	57	theme	plant-based	1026:1036	arg1	beads					1057:1061	0.05g plant-based chitosan composite beads	1020:1061	0.05g plant-based chitosan composite beads	1020:1061	Even at the high B concentration (8mgBL-1), B in drinking water could be reduced to less than 2.4mgL -1 when 0.05g plant-based chitosan composite beads and 12 Lemna fronds were used for 50mL test solution.
28385225	4	58	theme	adsorption	604:613	arg1	experiment					615:624	batch adsorption experiment	598:624	batch adsorption experiment	598:624	Our results from batch adsorption experiment indicated that a plant-based chitosan composite bead has a higher capacity of B removal than mineral-based chitosan composite beads.
28385225	3	59	theme	composite	496:504	arg1	beads					506:510	various chitosan composite beads	479:510	various chitosan composite beads	479:510	In the present study, an innovative and environment friendly method based on hybrid systems consisting of various chitosan composite beads and Lemna gibba were evaluated for removal of B from drinking water.
28385225	3	60	theme	environment	413:423	arg1	method					434:439	an innovative and environment friendly method	395:439	method	434:439	In the present study, an innovative and environment friendly method based on hybrid systems consisting of various chitosan composite beads and Lemna gibba were evaluated for removal of B from drinking water.
28385225	4	61	contain	has	679:681	arg2	capacity					692:699	a higher capacity	683:699	a higher capacity of B removal	683:712	Our results from batch adsorption experiment indicated that a plant-based chitosan composite bead has a higher capacity of B removal than mineral-based chitosan composite beads.
28385225	4	61	contain	has	679:681	arg1	bead					674:677	a plant-based chitosan composite bead	641:677	a plant-based chitosan composite bead	641:677	Our results from batch adsorption experiment indicated that a plant-based chitosan composite bead has a higher capacity of B removal than mineral-based chitosan composite beads.
28385225	2	62	theme	treatment	336:344	arg1	techniques					346:355	alternative and cost effective treatment techniques	305:355	alternative and cost effective treatment techniques	305:355	Clearly, alternative and cost effective treatment techniques are imperative.
28837839	6	0	theme	polymerase	1029:1038	arg1	Wzy					1040:1042	the unusually divergent repeat unit polymerase Wzy	993:1042	the unusually divergent repeat unit polymerase Wzy in the 23A cps biosynthesis locus	993:1076	This is due to the different polymerization position catalysed by the unusually divergent repeat unit polymerase Wzy in the 23A cps biosynthesis locus.
28837839	3	1	theme	linkage	569:575	arg1	analysis					577:584	linkage analysis	569:584	linkage analysis	569:584	Here, we report the previously unknown structures of the pneumococcal capsular polysaccharides serotype 23A and 23B determined using genetic analysis, NMR spectroscopy, composition and linkage analysis and Smith degradation (of polysaccharide 23A).
28837839	4	2	theme	polysaccharide	676:689	arg1	-β-L-Rhap-					761:770	: →4)-β-D-Glcp-(1→3)-[[α-L-Rhap-(1→2)]-[Gro-(2→P→3)]-β-D-Galp-(1→4)]-β-L-Rhap-(1→	693:773	: →4)-β-D-Glcp-(1→3)-[[α-L-Rhap-(1→2)]-[Gro-(2→P→3)]-β-D-Galp-(1→4)]-β-L-Rhap-(1→	693:773	The structure of the serotype 23A capsular polysaccharide is: →4)-β-D-Glcp-(1→3)-[[α-L-Rhap-(1→2)]-[Gro-(2→P→3)]-β-D-Galp-(1→4)]-β-L-Rhap-(1→.
28837839	4	2	theme	polysaccharide	676:689	arg1	structure					637:645	The structure	633:645	The structure of the serotype 23A capsular polysaccharide	633:689	The structure of the serotype 23A capsular polysaccharide is: →4)-β-D-Glcp-(1→3)-[[α-L-Rhap-(1→2)]-[Gro-(2→P→3)]-β-D-Galp-(1→4)]-β-L-Rhap-(1→.
28837839	5	3	theme	polysaccharide	804:817	arg1	23F					819:821	polysaccharide 23F	804:821	polysaccharide 23F	804:821	This structure differs from polysaccharide 23F as it features a disaccharide backbone and the di-substituted β-Gal is linked to β-Rha as a side chain.
28837839	2	4	theme	licensed	329:336	arg1	vaccines					338:345	licensed vaccines	329:345	licensed vaccines	329:345	Serogroup 23 consists of serotype 23F, present in licensed vaccines, and emerging serotypes 23A and 23B.
28837839	1	5	theme	encapsulated	196:207	arg1	pathogen					215:222	a globally important encapsulated human pathogen	175:222	a globally important encapsulated human pathogen with approximately 100 different serotypes recognized	175:276	Streptococcus pneumoniae is a globally important encapsulated human pathogen with approximately 100 different serotypes recognized.
28837839	1	5	theme	encapsulated	196:207	arg1	pneumoniae					161:170	Streptococcus pneumoniae	147:170	Streptococcus pneumoniae	147:170	Streptococcus pneumoniae is a globally important encapsulated human pathogen with approximately 100 different serotypes recognized.
28837839	10	6	with	slight	1571:1576	arg1	23A					1583:1585	23A	1583:1585	23A	1583:1585	This may explain the reported typing cross reactions for serotype 23F: slight with 23A and none with 23B.
28837839	4	7	theme	23A	663:665	arg1	polysaccharide					676:689	the serotype 23A capsular polysaccharide	650:689	the serotype 23A capsular polysaccharide	650:689	The structure of the serotype 23A capsular polysaccharide is: →4)-β-D-Glcp-(1→3)-[[α-L-Rhap-(1→2)]-[Gro-(2→P→3)]-β-D-Galp-(1→4)]-β-L-Rhap-(1→.
28837839	0	8	theme	serotype	133:140	arg1	23F					142:144	serotype 23F	133:144	serotype 23F	133:144	Genetic and structural elucidation of capsular polysaccharides from Streptococcus pneumoniae serotype 23A and 23B, and comparison to serotype 23F.
28837839	3	9	theme	23A	627:629	arg1	spectroscopy					539:550	NMR spectroscopy	535:550	NMR spectroscopy	535:550	Here, we report the previously unknown structures of the pneumococcal capsular polysaccharides serotype 23A and 23B determined using genetic analysis, NMR spectroscopy, composition and linkage analysis and Smith degradation (of polysaccharide 23A).
28837839	3	9	theme	23A	627:629	arg1	degradation					596:606	Smith degradation	590:606	Smith degradation	590:606	Here, we report the previously unknown structures of the pneumococcal capsular polysaccharides serotype 23A and 23B determined using genetic analysis, NMR spectroscopy, composition and linkage analysis and Smith degradation (of polysaccharide 23A).
28837839	3	9	theme	23A	627:629	arg1	analysis					577:584	linkage analysis	569:584	linkage analysis	569:584	Here, we report the previously unknown structures of the pneumococcal capsular polysaccharides serotype 23A and 23B determined using genetic analysis, NMR spectroscopy, composition and linkage analysis and Smith degradation (of polysaccharide 23A).
28837839	3	9	theme	23A	627:629	arg1	analysis					525:532	genetic analysis	517:532	genetic analysis	517:532	Here, we report the previously unknown structures of the pneumococcal capsular polysaccharides serotype 23A and 23B determined using genetic analysis, NMR spectroscopy, composition and linkage analysis and Smith degradation (of polysaccharide 23A).
28837839	3	9	theme	23A	627:629	arg1	composition					553:563	composition	553:563	composition	553:563	Here, we report the previously unknown structures of the pneumococcal capsular polysaccharides serotype 23A and 23B determined using genetic analysis, NMR spectroscopy, composition and linkage analysis and Smith degradation (of polysaccharide 23A).
28837839	9	10	from	absent	1485:1490	arg1	23B					1495:1497	23B	1495:1497	23B	1495:1497	The immunodominant terminal α-Rha of 23F is more sterically crowded in 23A and absent in 23B.
28837839	9	11	from	23B	1495:1497	arg1	absent					1485:1490	absent	1485:1490	absent	1485:1490	The immunodominant terminal α-Rha of 23F is more sterically crowded in 23A and absent in 23B.
28837839	5	12	attach	linked	894:899	arg2	β-Gal					885:889	the di-substituted β-Gal	866:889	the di-substituted β-Gal	866:889	This structure differs from polysaccharide 23F as it features a disaccharide backbone and the di-substituted β-Gal is linked to β-Rha as a side chain.
28837839	5	12	attach	linked	894:899	arg1	β-Rha					904:908	β-Rha	904:908	β-Rha	904:908	This structure differs from polysaccharide 23F as it features a disaccharide backbone and the di-substituted β-Gal is linked to β-Rha as a side chain.
28837839	5	12	attach	linked	894:899	arg2	chain					920:924	a side chain	913:924	a side chain	913:924	This structure differs from polysaccharide 23F as it features a disaccharide backbone and the di-substituted β-Gal is linked to β-Rha as a side chain.
28837839	4	13	theme	-[Gro-	731:736	arg1	-β-L-Rhap-					761:770	: →4)-β-D-Glcp-(1→3)-[[α-L-Rhap-(1→2)]-[Gro-(2→P→3)]-β-D-Galp-(1→4)]-β-L-Rhap-(1→	693:773	: →4)-β-D-Glcp-(1→3)-[[α-L-Rhap-(1→2)]-[Gro-(2→P→3)]-β-D-Galp-(1→4)]-β-L-Rhap-(1→	693:773	The structure of the serotype 23A capsular polysaccharide is: →4)-β-D-Glcp-(1→3)-[[α-L-Rhap-(1→2)]-[Gro-(2→P→3)]-β-D-Galp-(1→4)]-β-L-Rhap-(1→.
28837839	4	13	theme	-[Gro-	731:736	arg1	structure					637:645	The structure	633:645	The structure of the serotype 23A capsular polysaccharide	633:689	The structure of the serotype 23A capsular polysaccharide is: →4)-β-D-Glcp-(1→3)-[[α-L-Rhap-(1→2)]-[Gro-(2→P→3)]-β-D-Galp-(1→4)]-β-L-Rhap-(1→.
28837839	5	14	theme	di-substituted	870:883	arg1	β-Gal					885:889	the di-substituted β-Gal	866:889	the di-substituted β-Gal	866:889	This structure differs from polysaccharide 23F as it features a disaccharide backbone and the di-substituted β-Gal is linked to β-Rha as a side chain.
28837839	5	14	theme	di-substituted	870:883	arg1	chain					920:924	a side chain	913:924	a side chain	913:924	This structure differs from polysaccharide 23F as it features a disaccharide backbone and the di-substituted β-Gal is linked to β-Rha as a side chain.
28837839	0	15	theme	Streptococcus	68:80	arg1	23A					102:104	Streptococcus pneumoniae serotype 23A	68:104	Streptococcus pneumoniae serotype 23A	68:104	Genetic and structural elucidation of capsular polysaccharides from Streptococcus pneumoniae serotype 23A and 23B, and comparison to serotype 23F.
28837839	1	16	theme	Streptococcus	147:159	arg1	pathogen					215:222	a globally important encapsulated human pathogen	175:222	a globally important encapsulated human pathogen with approximately 100 different serotypes recognized	175:276	Streptococcus pneumoniae is a globally important encapsulated human pathogen with approximately 100 different serotypes recognized.
28837839	1	16	theme	Streptococcus	147:159	arg1	pneumoniae					161:170	Streptococcus pneumoniae	147:170	Streptococcus pneumoniae	147:170	Streptococcus pneumoniae is a globally important encapsulated human pathogen with approximately 100 different serotypes recognized.
28837839	0	17	theme	serotype	93:100	arg1	23A					102:104	Streptococcus pneumoniae serotype 23A	68:104	Streptococcus pneumoniae serotype 23A	68:104	Genetic and structural elucidation of capsular polysaccharides from Streptococcus pneumoniae serotype 23A and 23B, and comparison to serotype 23F.
28837839	0	18	from	23B	110:112	arg1	comparison					119:128	comparison	119:128	comparison to serotype 23F	119:144	Genetic and structural elucidation of capsular polysaccharides from Streptococcus pneumoniae serotype 23A and 23B, and comparison to serotype 23F.
28837839	0	18	from	23B	110:112	arg1	elucidation					23:33	Genetic and structural elucidation	0:33	Genetic and structural elucidation of capsular polysaccharides from Streptococcus pneumoniae serotype 23A and 23B	0:112	Genetic and structural elucidation of capsular polysaccharides from Streptococcus pneumoniae serotype 23A and 23B, and comparison to serotype 23F.
28837839	0	18	from	23B	110:112	arg1	polysaccharides					47:61	capsular polysaccharides	38:61	capsular polysaccharides from Streptococcus pneumoniae serotype 23A and 23B	38:112	Genetic and structural elucidation of capsular polysaccharides from Streptococcus pneumoniae serotype 23A and 23B, and comparison to serotype 23F.
28837839	5	19	theme	disaccharide	840:851	arg1	backbone					853:860	a disaccharide backbone	838:860	a disaccharide backbone	838:860	This structure differs from polysaccharide 23F as it features a disaccharide backbone and the di-substituted β-Gal is linked to β-Rha as a side chain.
28837839	2	20	from	vaccines	338:345	arg1	present					318:324	present	318:324	present	318:324	Serogroup 23 consists of serotype 23F, present in licensed vaccines, and emerging serotypes 23A and 23B.
28837839	7	21	from	crowding	1086:1093	arg1	23A					1098:1100	23A	1098:1100	23A	1098:1100	Steric crowding in 23A, confirmed by molecular models, causes the NMR signal for H-1 of the di-substituted 2,3-β-Gal to resonate in the α-anomeric region.
28837839	8	22	theme	serotype	1255:1262	arg1	polysaccharide					1277:1290	the serotype 23B capsular polysaccharide	1251:1290	the serotype 23B capsular polysaccharide	1251:1290	The structure of the serotype 23B capsular polysaccharide is the same as 23F, but without the terminal α-Rha: →4)-β-D-Glcp-(1→4)-[Gro-(2→P→3)]-β-D-Galp-(1→4)-β-L-Rhap-(1→.
28837839	8	23	theme	-β-D-Galp-	1376:1385	arg1	1→					1402:1403	→4)-β-D-Glcp-(1→4)-[Gro-(2→P→3)]-β-D-Galp-(1→4)-β-L-Rhap-(1→	1344:1403	the terminal α-Rha: →4)-β-D-Glcp-(1→4)-[Gro-(2→P→3)]-β-D-Galp-(1→4)-β-L-Rhap-(1→	1324:1403	The structure of the serotype 23B capsular polysaccharide is the same as 23F, but without the terminal α-Rha: →4)-β-D-Glcp-(1→4)-[Gro-(2→P→3)]-β-D-Galp-(1→4)-β-L-Rhap-(1→.
28837839	8	24	theme	capsular	1268:1275	arg1	polysaccharide					1277:1290	the serotype 23B capsular polysaccharide	1251:1290	the serotype 23B capsular polysaccharide	1251:1290	The structure of the serotype 23B capsular polysaccharide is the same as 23F, but without the terminal α-Rha: →4)-β-D-Glcp-(1→4)-[Gro-(2→P→3)]-β-D-Galp-(1→4)-β-L-Rhap-(1→.
28837839	3	25	theme	pneumococcal	441:452	arg1	polysaccharides					463:477	the pneumococcal capsular polysaccharides	437:477	the pneumococcal capsular polysaccharides	437:477	Here, we report the previously unknown structures of the pneumococcal capsular polysaccharides serotype 23A and 23B determined using genetic analysis, NMR spectroscopy, composition and linkage analysis and Smith degradation (of polysaccharide 23A).
28837839	7	26	theme	molecular	1116:1124	arg1	models					1126:1131	molecular models	1116:1131	molecular models	1116:1131	Steric crowding in 23A, confirmed by molecular models, causes the NMR signal for H-1 of the di-substituted 2,3-β-Gal to resonate in the α-anomeric region.
28837839	0	27	theme	capsular	38:45	arg1	polysaccharides					47:61	capsular polysaccharides	38:61	capsular polysaccharides from Streptococcus pneumoniae serotype 23A and 23B	38:112	Genetic and structural elucidation of capsular polysaccharides from Streptococcus pneumoniae serotype 23A and 23B, and comparison to serotype 23F.
28837839	5	28	theme	side	915:918	arg1	chain					920:924	a side chain	913:924	a side chain	913:924	This structure differs from polysaccharide 23F as it features a disaccharide backbone and the di-substituted β-Gal is linked to β-Rha as a side chain.
28837839	5	28	theme	side	915:918	arg1	β-Gal					885:889	the di-substituted β-Gal	866:889	the di-substituted β-Gal	866:889	This structure differs from polysaccharide 23F as it features a disaccharide backbone and the di-substituted β-Gal is linked to β-Rha as a side chain.
28837839	6	29	theme	divergent	1007:1015	arg1	Wzy					1040:1042	the unusually divergent repeat unit polymerase Wzy	993:1042	the unusually divergent repeat unit polymerase Wzy in the 23A cps biosynthesis locus	993:1076	This is due to the different polymerization position catalysed by the unusually divergent repeat unit polymerase Wzy in the 23A cps biosynthesis locus.
28837839	8	30	theme	1→4	1387:1389	arg1	1→					1402:1403	→4)-β-D-Glcp-(1→4)-[Gro-(2→P→3)]-β-D-Galp-(1→4)-β-L-Rhap-(1→	1344:1403	the terminal α-Rha: →4)-β-D-Glcp-(1→4)-[Gro-(2→P→3)]-β-D-Galp-(1→4)-β-L-Rhap-(1→	1324:1403	The structure of the serotype 23B capsular polysaccharide is the same as 23F, but without the terminal α-Rha: →4)-β-D-Glcp-(1→4)-[Gro-(2→P→3)]-β-D-Galp-(1→4)-β-L-Rhap-(1→.
28837839	0	31	theme	Genetic	0:6	arg1	elucidation					23:33	Genetic and structural elucidation	0:33	Genetic and structural elucidation of capsular polysaccharides from Streptococcus pneumoniae serotype 23A and 23B	0:112	Genetic and structural elucidation of capsular polysaccharides from Streptococcus pneumoniae serotype 23A and 23B, and comparison to serotype 23F.
28837839	9	32	theme	immunodominant	1410:1423	arg1	crowded					1466:1472	crowded	1466:1472	crowded	1466:1472	The immunodominant terminal α-Rha of 23F is more sterically crowded in 23A and absent in 23B.
28837839	9	32	theme	immunodominant	1410:1423	arg1	α-Rha					1434:1438	The immunodominant terminal α-Rha	1406:1438	The immunodominant terminal α-Rha of 23F	1406:1445	The immunodominant terminal α-Rha of 23F is more sterically crowded in 23A and absent in 23B.
28837839	8	33	theme	-β-L-Rhap-	1391:1400	arg1	1→					1402:1403	→4)-β-D-Glcp-(1→4)-[Gro-(2→P→3)]-β-D-Galp-(1→4)-β-L-Rhap-(1→	1344:1403	the terminal α-Rha: →4)-β-D-Glcp-(1→4)-[Gro-(2→P→3)]-β-D-Galp-(1→4)-β-L-Rhap-(1→	1324:1403	The structure of the serotype 23B capsular polysaccharide is the same as 23F, but without the terminal α-Rha: →4)-β-D-Glcp-(1→4)-[Gro-(2→P→3)]-β-D-Galp-(1→4)-β-L-Rhap-(1→.
28837839	2	34	dep	serotypes	361:369	arg1	serotypes					361:369	serotypes	361:369	serotypes 23A and 23B	361:381	Serogroup 23 consists of serotype 23F, present in licensed vaccines, and emerging serotypes 23A and 23B.
28837839	2	34	dep	serotypes	361:369	arg1	23B					379:381	23B	379:381	23B	379:381	Serogroup 23 consists of serotype 23F, present in licensed vaccines, and emerging serotypes 23A and 23B.
28837839	2	34	dep	serotypes	361:369	arg1	23A					371:373	23A	371:373	23A	371:373	Serogroup 23 consists of serotype 23F, present in licensed vaccines, and emerging serotypes 23A and 23B.
28837839	0	35	theme	structural	12:21	arg1	elucidation					23:33	Genetic and structural elucidation	0:33	Genetic and structural elucidation of capsular polysaccharides from Streptococcus pneumoniae serotype 23A and 23B	0:112	Genetic and structural elucidation of capsular polysaccharides from Streptococcus pneumoniae serotype 23A and 23B, and comparison to serotype 23F.
28837839	0	36	dep	Streptococcus	68:80	arg1	pneumoniae					82:91	pneumoniae	82:91	pneumoniae	82:91	Genetic and structural elucidation of capsular polysaccharides from Streptococcus pneumoniae serotype 23A and 23B, and comparison to serotype 23F.
28837839	3	37	theme	unknown	415:421	arg1	23B					496:498	23B	496:498	23B	496:498	Here, we report the previously unknown structures of the pneumococcal capsular polysaccharides serotype 23A and 23B determined using genetic analysis, NMR spectroscopy, composition and linkage analysis and Smith degradation (of polysaccharide 23A).
28837839	3	37	theme	unknown	415:421	arg1	23A					488:490	serotype 23A	479:490	serotype 23A	479:490	Here, we report the previously unknown structures of the pneumococcal capsular polysaccharides serotype 23A and 23B determined using genetic analysis, NMR spectroscopy, composition and linkage analysis and Smith degradation (of polysaccharide 23A).
28837839	3	37	theme	unknown	415:421	arg1	structures					423:432	the previously unknown structures	400:432	the previously unknown structures of the pneumococcal capsular polysaccharides serotype 23A and 23B determined using genetic analysis, NMR spectroscopy, composition and linkage analysis and Smith degradation (of polysaccharide 23A)	400:630	Here, we report the previously unknown structures of the pneumococcal capsular polysaccharides serotype 23A and 23B determined using genetic analysis, NMR spectroscopy, composition and linkage analysis and Smith degradation (of polysaccharide 23A).
28837839	3	38	theme	polysaccharides	463:477	arg1	23B					496:498	23B	496:498	23B	496:498	Here, we report the previously unknown structures of the pneumococcal capsular polysaccharides serotype 23A and 23B determined using genetic analysis, NMR spectroscopy, composition and linkage analysis and Smith degradation (of polysaccharide 23A).
28837839	3	38	theme	polysaccharides	463:477	arg1	23A					488:490	serotype 23A	479:490	serotype 23A	479:490	Here, we report the previously unknown structures of the pneumococcal capsular polysaccharides serotype 23A and 23B determined using genetic analysis, NMR spectroscopy, composition and linkage analysis and Smith degradation (of polysaccharide 23A).
28837839	3	38	theme	polysaccharides	463:477	arg1	structures					423:432	the previously unknown structures	400:432	the previously unknown structures of the pneumococcal capsular polysaccharides serotype 23A and 23B determined using genetic analysis, NMR spectroscopy, composition and linkage analysis and Smith degradation (of polysaccharide 23A)	400:630	Here, we report the previously unknown structures of the pneumococcal capsular polysaccharides serotype 23A and 23B determined using genetic analysis, NMR spectroscopy, composition and linkage analysis and Smith degradation (of polysaccharide 23A).
28837839	1	39	with	pathogen	215:222	arg1	serotypes					257:265	approximately 100 different serotypes	229:265	approximately 100 different serotypes recognized	229:276	Streptococcus pneumoniae is a globally important encapsulated human pathogen with approximately 100 different serotypes recognized.
28837839	9	40	theme	23F	1443:1445	arg1	crowded					1466:1472	crowded	1466:1472	crowded	1466:1472	The immunodominant terminal α-Rha of 23F is more sterically crowded in 23A and absent in 23B.
28837839	9	40	theme	23F	1443:1445	arg1	α-Rha					1434:1438	The immunodominant terminal α-Rha	1406:1438	The immunodominant terminal α-Rha of 23F	1406:1445	The immunodominant terminal α-Rha of 23F is more sterically crowded in 23A and absent in 23B.
28837839	6	41	theme	polymerization	956:969	arg1	position					971:978	the different polymerization position	942:978	the different polymerization position catalysed by the unusually divergent repeat unit polymerase Wzy in the 23A cps biosynthesis locus	942:1076	This is due to the different polymerization position catalysed by the unusually divergent repeat unit polymerase Wzy in the 23A cps biosynthesis locus.
28837839	10	42	theme	cross	1537:1541	arg1	reactions					1543:1551	the reported typing cross reactions	1517:1551	the reported typing cross reactions for serotype 23F: slight with 23A and none with 23B	1517:1603	This may explain the reported typing cross reactions for serotype 23F: slight with 23A and none with 23B.
28837839	0	43	theme	polysaccharides	47:61	arg1	comparison					119:128	comparison	119:128	comparison to serotype 23F	119:144	Genetic and structural elucidation of capsular polysaccharides from Streptococcus pneumoniae serotype 23A and 23B, and comparison to serotype 23F.
28837839	0	43	theme	polysaccharides	47:61	arg1	elucidation					23:33	Genetic and structural elucidation	0:33	Genetic and structural elucidation of capsular polysaccharides from Streptococcus pneumoniae serotype 23A and 23B	0:112	Genetic and structural elucidation of capsular polysaccharides from Streptococcus pneumoniae serotype 23A and 23B, and comparison to serotype 23F.
28837839	0	44	from	elucidation	23:33	arg1	23B					110:112	23B	110:112	23B	110:112	Genetic and structural elucidation of capsular polysaccharides from Streptococcus pneumoniae serotype 23A and 23B, and comparison to serotype 23F.
28837839	0	44	from	elucidation	23:33	arg1	23A					102:104	Streptococcus pneumoniae serotype 23A	68:104	Streptococcus pneumoniae serotype 23A	68:104	Genetic and structural elucidation of capsular polysaccharides from Streptococcus pneumoniae serotype 23A and 23B, and comparison to serotype 23F.
28837839	6	45	theme	cps	1055:1057	arg1	locus					1072:1076	the 23A cps biosynthesis locus	1047:1076	the 23A cps biosynthesis locus	1047:1076	This is due to the different polymerization position catalysed by the unusually divergent repeat unit polymerase Wzy in the 23A cps biosynthesis locus.
28837839	10	46	theme	reported	1521:1528	arg1	reactions					1543:1551	the reported typing cross reactions	1517:1551	the reported typing cross reactions for serotype 23F: slight with 23A and none with 23B	1517:1603	This may explain the reported typing cross reactions for serotype 23F: slight with 23A and none with 23B.
28837839	7	47	theme	2,3-β-Gal	1186:1194	arg1	H-1					1160:1162	H-1	1160:1162	H-1 of the di-substituted 2,3-β-Gal	1160:1194	Steric crowding in 23A, confirmed by molecular models, causes the NMR signal for H-1 of the di-substituted 2,3-β-Gal to resonate in the α-anomeric region.
28837839	3	48	theme	NMR	535:537	arg1	spectroscopy					539:550	NMR spectroscopy	535:550	NMR spectroscopy	535:550	Here, we report the previously unknown structures of the pneumococcal capsular polysaccharides serotype 23A and 23B determined using genetic analysis, NMR spectroscopy, composition and linkage analysis and Smith degradation (of polysaccharide 23A).
28837839	0	49	from	comparison	119:128	arg1	23B					110:112	23B	110:112	23B	110:112	Genetic and structural elucidation of capsular polysaccharides from Streptococcus pneumoniae serotype 23A and 23B, and comparison to serotype 23F.
28837839	0	49	from	comparison	119:128	arg1	23A					102:104	Streptococcus pneumoniae serotype 23A	68:104	Streptococcus pneumoniae serotype 23A	68:104	Genetic and structural elucidation of capsular polysaccharides from Streptococcus pneumoniae serotype 23A and 23B, and comparison to serotype 23F.
28837839	6	50	from	Wzy	1040:1042	arg1	locus					1072:1076	the 23A cps biosynthesis locus	1047:1076	the 23A cps biosynthesis locus	1047:1076	This is due to the different polymerization position catalysed by the unusually divergent repeat unit polymerase Wzy in the 23A cps biosynthesis locus.
28837839	6	51	theme	unit	1024:1027	arg1	Wzy					1040:1042	the unusually divergent repeat unit polymerase Wzy	993:1042	the unusually divergent repeat unit polymerase Wzy in the 23A cps biosynthesis locus	993:1076	This is due to the different polymerization position catalysed by the unusually divergent repeat unit polymerase Wzy in the 23A cps biosynthesis locus.
28837839	2	52	theme	serotype	304:311	arg1	23F					313:315	serotype 23F	304:315	serotype 23F	304:315	Serogroup 23 consists of serotype 23F, present in licensed vaccines, and emerging serotypes 23A and 23B.
28837839	4	53	theme	capsular	667:674	arg1	polysaccharide					676:689	the serotype 23A capsular polysaccharide	650:689	the serotype 23A capsular polysaccharide	650:689	The structure of the serotype 23A capsular polysaccharide is: →4)-β-D-Glcp-(1→3)-[[α-L-Rhap-(1→2)]-[Gro-(2→P→3)]-β-D-Galp-(1→4)]-β-L-Rhap-(1→.
28837839	3	54	theme	Smith	590:594	arg1	degradation					596:606	Smith degradation	590:606	Smith degradation	590:606	Here, we report the previously unknown structures of the pneumococcal capsular polysaccharides serotype 23A and 23B determined using genetic analysis, NMR spectroscopy, composition and linkage analysis and Smith degradation (of polysaccharide 23A).
28837839	1	55	theme	important	186:194	arg1	pathogen					215:222	a globally important encapsulated human pathogen	175:222	a globally important encapsulated human pathogen with approximately 100 different serotypes recognized	175:276	Streptococcus pneumoniae is a globally important encapsulated human pathogen with approximately 100 different serotypes recognized.
28837839	1	55	theme	important	186:194	arg1	pneumoniae					161:170	Streptococcus pneumoniae	147:170	Streptococcus pneumoniae	147:170	Streptococcus pneumoniae is a globally important encapsulated human pathogen with approximately 100 different serotypes recognized.
28837839	1	56	theme	human	209:213	arg1	pathogen					215:222	a globally important encapsulated human pathogen	175:222	a globally important encapsulated human pathogen with approximately 100 different serotypes recognized	175:276	Streptococcus pneumoniae is a globally important encapsulated human pathogen with approximately 100 different serotypes recognized.
28837839	1	56	theme	human	209:213	arg1	pneumoniae					161:170	Streptococcus pneumoniae	147:170	Streptococcus pneumoniae	147:170	Streptococcus pneumoniae is a globally important encapsulated human pathogen with approximately 100 different serotypes recognized.
28837839	2	57	attach	present	318:324	arg2	Serogroup					279:287	Serogroup 23	279:290	Serogroup 23	279:290	Serogroup 23 consists of serotype 23F, present in licensed vaccines, and emerging serotypes 23A and 23B.
28837839	2	57	attach	present	318:324	arg1	vaccines					338:345	licensed vaccines	329:345	licensed vaccines	329:345	Serogroup 23 consists of serotype 23F, present in licensed vaccines, and emerging serotypes 23A and 23B.
28837839	8	58	theme	→4	1344:1345	arg1	1→					1402:1403	→4)-β-D-Glcp-(1→4)-[Gro-(2→P→3)]-β-D-Galp-(1→4)-β-L-Rhap-(1→	1344:1403	the terminal α-Rha: →4)-β-D-Glcp-(1→4)-[Gro-(2→P→3)]-β-D-Galp-(1→4)-β-L-Rhap-(1→	1324:1403	The structure of the serotype 23B capsular polysaccharide is the same as 23F, but without the terminal α-Rha: →4)-β-D-Glcp-(1→4)-[Gro-(2→P→3)]-β-D-Galp-(1→4)-β-L-Rhap-(1→.
28837839	10	59	theme	typing	1530:1535	arg1	reactions					1543:1551	the reported typing cross reactions	1517:1551	the reported typing cross reactions for serotype 23F: slight with 23A and none with 23B	1517:1603	This may explain the reported typing cross reactions for serotype 23F: slight with 23A and none with 23B.
28837839	3	60	theme	polysaccharide	612:625	arg1	23A					627:629	polysaccharide 23A	612:629	polysaccharide 23A	612:629	Here, we report the previously unknown structures of the pneumococcal capsular polysaccharides serotype 23A and 23B determined using genetic analysis, NMR spectroscopy, composition and linkage analysis and Smith degradation (of polysaccharide 23A).
28837839	4	61	dep	-β-L-Rhap-	761:770	arg1	1→4					756:758	1→4	756:758	1→4	756:758	The structure of the serotype 23A capsular polysaccharide is: →4)-β-D-Glcp-(1→3)-[[α-L-Rhap-(1→2)]-[Gro-(2→P→3)]-β-D-Galp-(1→4)]-β-L-Rhap-(1→.
28837839	4	61	dep	-β-L-Rhap-	761:770	arg1	2→P→3					738:742	2→P→3	738:742	2→P→3	738:742	The structure of the serotype 23A capsular polysaccharide is: →4)-β-D-Glcp-(1→3)-[[α-L-Rhap-(1→2)]-[Gro-(2→P→3)]-β-D-Galp-(1→4)]-β-L-Rhap-(1→.
28837839	4	61	dep	-β-L-Rhap-	761:770	arg1	1→2					726:728	1→2	726:728	1→2	726:728	The structure of the serotype 23A capsular polysaccharide is: →4)-β-D-Glcp-(1→3)-[[α-L-Rhap-(1→2)]-[Gro-(2→P→3)]-β-D-Galp-(1→4)]-β-L-Rhap-(1→.
28837839	8	62	theme	-β-D-Glcp-	1347:1356	arg1	1→					1402:1403	→4)-β-D-Glcp-(1→4)-[Gro-(2→P→3)]-β-D-Galp-(1→4)-β-L-Rhap-(1→	1344:1403	the terminal α-Rha: →4)-β-D-Glcp-(1→4)-[Gro-(2→P→3)]-β-D-Galp-(1→4)-β-L-Rhap-(1→	1324:1403	The structure of the serotype 23B capsular polysaccharide is the same as 23F, but without the terminal α-Rha: →4)-β-D-Glcp-(1→4)-[Gro-(2→P→3)]-β-D-Galp-(1→4)-β-L-Rhap-(1→.
28837839	4	63	theme	serotype	654:661	arg1	polysaccharide					676:689	the serotype 23A capsular polysaccharide	650:689	the serotype 23A capsular polysaccharide	650:689	The structure of the serotype 23A capsular polysaccharide is: →4)-β-D-Glcp-(1→3)-[[α-L-Rhap-(1→2)]-[Gro-(2→P→3)]-β-D-Galp-(1→4)]-β-L-Rhap-(1→.
28837839	8	64	dep	α-Rha	1337:1341	arg1	1→					1402:1403	→4)-β-D-Glcp-(1→4)-[Gro-(2→P→3)]-β-D-Galp-(1→4)-β-L-Rhap-(1→	1344:1403	the terminal α-Rha: →4)-β-D-Glcp-(1→4)-[Gro-(2→P→3)]-β-D-Galp-(1→4)-β-L-Rhap-(1→	1324:1403	The structure of the serotype 23B capsular polysaccharide is the same as 23F, but without the terminal α-Rha: →4)-β-D-Glcp-(1→4)-[Gro-(2→P→3)]-β-D-Galp-(1→4)-β-L-Rhap-(1→.
28837839	8	65	theme	1→4	1358:1360	arg1	1→					1402:1403	→4)-β-D-Glcp-(1→4)-[Gro-(2→P→3)]-β-D-Galp-(1→4)-β-L-Rhap-(1→	1344:1403	the terminal α-Rha: →4)-β-D-Glcp-(1→4)-[Gro-(2→P→3)]-β-D-Galp-(1→4)-β-L-Rhap-(1→	1324:1403	The structure of the serotype 23B capsular polysaccharide is the same as 23F, but without the terminal α-Rha: →4)-β-D-Glcp-(1→4)-[Gro-(2→P→3)]-β-D-Galp-(1→4)-β-L-Rhap-(1→.
28837839	6	66	theme	different	946:954	arg1	position					971:978	the different polymerization position	942:978	the different polymerization position catalysed by the unusually divergent repeat unit polymerase Wzy in the 23A cps biosynthesis locus	942:1076	This is due to the different polymerization position catalysed by the unusually divergent repeat unit polymerase Wzy in the 23A cps biosynthesis locus.
28837839	8	67	theme	-[Gro-	1362:1367	arg1	1→					1402:1403	→4)-β-D-Glcp-(1→4)-[Gro-(2→P→3)]-β-D-Galp-(1→4)-β-L-Rhap-(1→	1344:1403	the terminal α-Rha: →4)-β-D-Glcp-(1→4)-[Gro-(2→P→3)]-β-D-Galp-(1→4)-β-L-Rhap-(1→	1324:1403	The structure of the serotype 23B capsular polysaccharide is the same as 23F, but without the terminal α-Rha: →4)-β-D-Glcp-(1→4)-[Gro-(2→P→3)]-β-D-Galp-(1→4)-β-L-Rhap-(1→.
28837839	4	68	theme	-[[α-L-Rhap-	713:724	arg1	-β-L-Rhap-					761:770	: →4)-β-D-Glcp-(1→3)-[[α-L-Rhap-(1→2)]-[Gro-(2→P→3)]-β-D-Galp-(1→4)]-β-L-Rhap-(1→	693:773	: →4)-β-D-Glcp-(1→3)-[[α-L-Rhap-(1→2)]-[Gro-(2→P→3)]-β-D-Galp-(1→4)]-β-L-Rhap-(1→	693:773	The structure of the serotype 23A capsular polysaccharide is: →4)-β-D-Glcp-(1→3)-[[α-L-Rhap-(1→2)]-[Gro-(2→P→3)]-β-D-Galp-(1→4)]-β-L-Rhap-(1→.
28837839	4	68	theme	-[[α-L-Rhap-	713:724	arg1	structure					637:645	The structure	633:645	The structure of the serotype 23A capsular polysaccharide	633:689	The structure of the serotype 23A capsular polysaccharide is: →4)-β-D-Glcp-(1→3)-[[α-L-Rhap-(1→2)]-[Gro-(2→P→3)]-β-D-Galp-(1→4)]-β-L-Rhap-(1→.
28837839	6	69	theme	23A	1051:1053	arg1	locus					1072:1076	the 23A cps biosynthesis locus	1047:1076	the 23A cps biosynthesis locus	1047:1076	This is due to the different polymerization position catalysed by the unusually divergent repeat unit polymerase Wzy in the 23A cps biosynthesis locus.
28837839	7	70	theme	NMR	1145:1147	arg1	signal					1149:1154	the NMR signal	1141:1154	the NMR signal for H-1 of the di-substituted 2,3-β-Gal to resonate in the α-anomeric region	1141:1231	Steric crowding in 23A, confirmed by molecular models, causes the NMR signal for H-1 of the di-substituted 2,3-β-Gal to resonate in the α-anomeric region.
28837839	8	71	theme	2→P→3	1369:1373	arg1	1→					1402:1403	→4)-β-D-Glcp-(1→4)-[Gro-(2→P→3)]-β-D-Galp-(1→4)-β-L-Rhap-(1→	1344:1403	the terminal α-Rha: →4)-β-D-Glcp-(1→4)-[Gro-(2→P→3)]-β-D-Galp-(1→4)-β-L-Rhap-(1→	1324:1403	The structure of the serotype 23B capsular polysaccharide is the same as 23F, but without the terminal α-Rha: →4)-β-D-Glcp-(1→4)-[Gro-(2→P→3)]-β-D-Galp-(1→4)-β-L-Rhap-(1→.
28837839	2	72	from	present	318:324	arg1	vaccines					338:345	licensed vaccines	329:345	licensed vaccines	329:345	Serogroup 23 consists of serotype 23F, present in licensed vaccines, and emerging serotypes 23A and 23B.
28837839	4	73	theme	-β-D-Glcp-	698:707	arg1	-β-L-Rhap-					761:770	: →4)-β-D-Glcp-(1→3)-[[α-L-Rhap-(1→2)]-[Gro-(2→P→3)]-β-D-Galp-(1→4)]-β-L-Rhap-(1→	693:773	: →4)-β-D-Glcp-(1→3)-[[α-L-Rhap-(1→2)]-[Gro-(2→P→3)]-β-D-Galp-(1→4)]-β-L-Rhap-(1→	693:773	The structure of the serotype 23A capsular polysaccharide is: →4)-β-D-Glcp-(1→3)-[[α-L-Rhap-(1→2)]-[Gro-(2→P→3)]-β-D-Galp-(1→4)]-β-L-Rhap-(1→.
28837839	4	73	theme	-β-D-Glcp-	698:707	arg1	structure					637:645	The structure	633:645	The structure of the serotype 23A capsular polysaccharide	633:689	The structure of the serotype 23A capsular polysaccharide is: →4)-β-D-Glcp-(1→3)-[[α-L-Rhap-(1→2)]-[Gro-(2→P→3)]-β-D-Galp-(1→4)]-β-L-Rhap-(1→.
28837839	0	74	from	23A	102:104	arg1	comparison					119:128	comparison	119:128	comparison to serotype 23F	119:144	Genetic and structural elucidation of capsular polysaccharides from Streptococcus pneumoniae serotype 23A and 23B, and comparison to serotype 23F.
28837839	0	74	from	23A	102:104	arg1	elucidation					23:33	Genetic and structural elucidation	0:33	Genetic and structural elucidation of capsular polysaccharides from Streptococcus pneumoniae serotype 23A and 23B	0:112	Genetic and structural elucidation of capsular polysaccharides from Streptococcus pneumoniae serotype 23A and 23B, and comparison to serotype 23F.
28837839	0	74	from	23A	102:104	arg1	polysaccharides					47:61	capsular polysaccharides	38:61	capsular polysaccharides from Streptococcus pneumoniae serotype 23A and 23B	38:112	Genetic and structural elucidation of capsular polysaccharides from Streptococcus pneumoniae serotype 23A and 23B, and comparison to serotype 23F.
28837839	10	75	dep	reactions	1543:1551	arg1	none					1591:1594	none	1591:1594	none	1591:1594	This may explain the reported typing cross reactions for serotype 23F: slight with 23A and none with 23B.
28837839	10	75	dep	reactions	1543:1551	arg1	slight					1571:1576	slight	1571:1576	slight	1571:1576	This may explain the reported typing cross reactions for serotype 23F: slight with 23A and none with 23B.
28837839	9	76	from	23A	1477:1479	arg1	crowded					1466:1472	crowded	1466:1472	crowded	1466:1472	The immunodominant terminal α-Rha of 23F is more sterically crowded in 23A and absent in 23B.
28837839	9	76	from	23A	1477:1479	arg1	α-Rha					1434:1438	The immunodominant terminal α-Rha	1406:1438	The immunodominant terminal α-Rha of 23F	1406:1445	The immunodominant terminal α-Rha of 23F is more sterically crowded in 23A and absent in 23B.
28837839	6	77	theme	repeat	1017:1022	arg1	Wzy					1040:1042	the unusually divergent repeat unit polymerase Wzy	993:1042	the unusually divergent repeat unit polymerase Wzy in the 23A cps biosynthesis locus	993:1076	This is due to the different polymerization position catalysed by the unusually divergent repeat unit polymerase Wzy in the 23A cps biosynthesis locus.
28837839	8	78	theme	23B	1264:1266	arg1	polysaccharide					1277:1290	the serotype 23B capsular polysaccharide	1251:1290	the serotype 23B capsular polysaccharide	1251:1290	The structure of the serotype 23B capsular polysaccharide is the same as 23F, but without the terminal α-Rha: →4)-β-D-Glcp-(1→4)-[Gro-(2→P→3)]-β-D-Galp-(1→4)-β-L-Rhap-(1→.
28837839	3	79	theme	capsular	454:461	arg1	polysaccharides					463:477	the pneumococcal capsular polysaccharides	437:477	the pneumococcal capsular polysaccharides	437:477	Here, we report the previously unknown structures of the pneumococcal capsular polysaccharides serotype 23A and 23B determined using genetic analysis, NMR spectroscopy, composition and linkage analysis and Smith degradation (of polysaccharide 23A).
28837839	9	80	from	crowded	1466:1472	arg1	23A					1477:1479	23A	1477:1479	23A	1477:1479	The immunodominant terminal α-Rha of 23F is more sterically crowded in 23A and absent in 23B.
28837839	3	81	dep	structures	423:432	arg1	23B					496:498	23B	496:498	23B	496:498	Here, we report the previously unknown structures of the pneumococcal capsular polysaccharides serotype 23A and 23B determined using genetic analysis, NMR spectroscopy, composition and linkage analysis and Smith degradation (of polysaccharide 23A).
28837839	3	81	dep	structures	423:432	arg1	23A					488:490	serotype 23A	479:490	serotype 23A	479:490	Here, we report the previously unknown structures of the pneumococcal capsular polysaccharides serotype 23A and 23B determined using genetic analysis, NMR spectroscopy, composition and linkage analysis and Smith degradation (of polysaccharide 23A).
28837839	3	81	dep	structures	423:432	arg1	structures					423:432	the previously unknown structures	400:432	the previously unknown structures of the pneumococcal capsular polysaccharides serotype 23A and 23B determined using genetic analysis, NMR spectroscopy, composition and linkage analysis and Smith degradation (of polysaccharide 23A)	400:630	Here, we report the previously unknown structures of the pneumococcal capsular polysaccharides serotype 23A and 23B determined using genetic analysis, NMR spectroscopy, composition and linkage analysis and Smith degradation (of polysaccharide 23A).
28837839	8	82	theme	polysaccharide	1277:1290	arg1	structure					1238:1246	The structure	1234:1246	The structure of the serotype 23B capsular polysaccharide	1234:1290	The structure of the serotype 23B capsular polysaccharide is the same as 23F, but without the terminal α-Rha: →4)-β-D-Glcp-(1→4)-[Gro-(2→P→3)]-β-D-Galp-(1→4)-β-L-Rhap-(1→.
28837839	8	82	theme	polysaccharide	1277:1290	arg1	same					1299:1302	same	1299:1302	same	1299:1302	The structure of the serotype 23B capsular polysaccharide is the same as 23F, but without the terminal α-Rha: →4)-β-D-Glcp-(1→4)-[Gro-(2→P→3)]-β-D-Galp-(1→4)-β-L-Rhap-(1→.
28837839	9	83	theme	terminal	1425:1432	arg1	crowded					1466:1472	crowded	1466:1472	crowded	1466:1472	The immunodominant terminal α-Rha of 23F is more sterically crowded in 23A and absent in 23B.
28837839	9	83	theme	terminal	1425:1432	arg1	α-Rha					1434:1438	The immunodominant terminal α-Rha	1406:1438	The immunodominant terminal α-Rha of 23F	1406:1445	The immunodominant terminal α-Rha of 23F is more sterically crowded in 23A and absent in 23B.
28837839	7	84	theme	α-anomeric	1215:1224	arg1	region					1226:1231	the α-anomeric region	1211:1231	the α-anomeric region	1211:1231	Steric crowding in 23A, confirmed by molecular models, causes the NMR signal for H-1 of the di-substituted 2,3-β-Gal to resonate in the α-anomeric region.
28837839	7	85	theme	Steric	1079:1084	arg1	crowding					1086:1093	Steric crowding	1079:1093	Steric crowding	1079:1093	Steric crowding in 23A, confirmed by molecular models, causes the NMR signal for H-1 of the di-substituted 2,3-β-Gal to resonate in the α-anomeric region.
28837839	3	86	theme	serotype	479:486	arg1	23A					488:490	serotype 23A	479:490	serotype 23A	479:490	Here, we report the previously unknown structures of the pneumococcal capsular polysaccharides serotype 23A and 23B determined using genetic analysis, NMR spectroscopy, composition and linkage analysis and Smith degradation (of polysaccharide 23A).
28837839	3	86	theme	serotype	479:486	arg1	structures					423:432	the previously unknown structures	400:432	the previously unknown structures of the pneumococcal capsular polysaccharides serotype 23A and 23B determined using genetic analysis, NMR spectroscopy, composition and linkage analysis and Smith degradation (of polysaccharide 23A)	400:630	Here, we report the previously unknown structures of the pneumococcal capsular polysaccharides serotype 23A and 23B determined using genetic analysis, NMR spectroscopy, composition and linkage analysis and Smith degradation (of polysaccharide 23A).
28837839	6	87	theme	biosynthesis	1059:1070	arg1	locus					1072:1076	the 23A cps biosynthesis locus	1047:1076	the 23A cps biosynthesis locus	1047:1076	This is due to the different polymerization position catalysed by the unusually divergent repeat unit polymerase Wzy in the 23A cps biosynthesis locus.
28837839	3	88	theme	genetic	517:523	arg1	analysis					525:532	genetic analysis	517:532	genetic analysis	517:532	Here, we report the previously unknown structures of the pneumococcal capsular polysaccharides serotype 23A and 23B determined using genetic analysis, NMR spectroscopy, composition and linkage analysis and Smith degradation (of polysaccharide 23A).
28837839	7	89	theme	di-substituted	1171:1184	arg1	2,3-β-Gal					1186:1194	the di-substituted 2,3-β-Gal	1167:1194	the di-substituted 2,3-β-Gal	1167:1194	Steric crowding in 23A, confirmed by molecular models, causes the NMR signal for H-1 of the di-substituted 2,3-β-Gal to resonate in the α-anomeric region.
28837839	4	90	theme	-β-D-Galp-	745:754	arg1	-β-L-Rhap-					761:770	: →4)-β-D-Glcp-(1→3)-[[α-L-Rhap-(1→2)]-[Gro-(2→P→3)]-β-D-Galp-(1→4)]-β-L-Rhap-(1→	693:773	: →4)-β-D-Glcp-(1→3)-[[α-L-Rhap-(1→2)]-[Gro-(2→P→3)]-β-D-Galp-(1→4)]-β-L-Rhap-(1→	693:773	The structure of the serotype 23A capsular polysaccharide is: →4)-β-D-Glcp-(1→3)-[[α-L-Rhap-(1→2)]-[Gro-(2→P→3)]-β-D-Galp-(1→4)]-β-L-Rhap-(1→.
28837839	4	90	theme	-β-D-Galp-	745:754	arg1	structure					637:645	The structure	633:645	The structure of the serotype 23A capsular polysaccharide	633:689	The structure of the serotype 23A capsular polysaccharide is: →4)-β-D-Glcp-(1→3)-[[α-L-Rhap-(1→2)]-[Gro-(2→P→3)]-β-D-Galp-(1→4)]-β-L-Rhap-(1→.
28837839	1	91	theme	different	247:255	arg1	serotypes					257:265	approximately 100 different serotypes	229:265	approximately 100 different serotypes recognized	229:276	Streptococcus pneumoniae is a globally important encapsulated human pathogen with approximately 100 different serotypes recognized.
28837839	8	92	theme	terminal	1328:1335	arg1	α-Rha					1337:1341	the terminal α-Rha	1324:1341	the terminal α-Rha: →4)-β-D-Glcp-(1→4)-[Gro-(2→P→3)]-β-D-Galp-(1→4)-β-L-Rhap-(1→	1324:1403	The structure of the serotype 23B capsular polysaccharide is the same as 23F, but without the terminal α-Rha: →4)-β-D-Glcp-(1→4)-[Gro-(2→P→3)]-β-D-Galp-(1→4)-β-L-Rhap-(1→.
28837839	10	93	theme	serotype	1557:1564	arg1	23F					1566:1568	serotype 23F	1557:1568	serotype 23F	1557:1568	This may explain the reported typing cross reactions for serotype 23F: slight with 23A and none with 23B.
24998774	5	0	theme	SaOS-2	856:861	arg1	cells					863:867	osteoblastic SaOS-2 cells	843:867	osteoblastic SaOS-2 cells	843:867	Finally, the cultivation of osteoblastic SaOS-2 cells on the surface-functionalized CHS-g-PCL was followed by analyses of cell viability, mineral deposition and alkaline phosphatase activity.
24998774	1	1	theme	tissue	355:360	arg1	engineering					362:372	tissue engineering	355:372	tissue engineering	355:372	Several attempts have been made in the past to fabricate hybrid materials that display the complementary properties of the polyester polycaprolactone (PCL) and the polysaccharide chitosan (CHS) for application in the field of bone regeneration and tissue engineering.
24998774	6	2	theme	used	1236:1239	arg1	composites					1249:1258	conventionally used CHS/PCL composites	1221:1258	conventionally used CHS/PCL composites	1221:1258	These characterizations revealed a composite that combines the versatile properties of CHS-g-PCL with the osteogenic activity of the silicatein/biosilica coating and, hence, represents an innovative alternative to conventionally used CHS/PCL composites for biomedical applications, where stable bone-material interfaces are required.
24998774	1	3	theme	chitosan	286:293	arg1	properties					212:221	the complementary properties	194:221	the complementary properties of the polyester polycaprolactone (PCL) and the polysaccharide chitosan (CHS) for application in the field of bone regeneration and tissue engineering	194:372	Several attempts have been made in the past to fabricate hybrid materials that display the complementary properties of the polyester polycaprolactone (PCL) and the polysaccharide chitosan (CHS) for application in the field of bone regeneration and tissue engineering.
24998774	5	4	theme	cells	863:867	arg1	cultivation					828:838	the cultivation	824:838	the cultivation of osteoblastic SaOS-2 cells on the surface-functionalized CHS-g-PCL	824:907	Finally, the cultivation of osteoblastic SaOS-2 cells on the surface-functionalized CHS-g-PCL was followed by analyses of cell viability, mineral deposition and alkaline phosphatase activity.
24998774	1	5	theme	engineering	362:372	arg1	field					324:328	the field	320:328	the field of bone regeneration and tissue engineering	320:372	Several attempts have been made in the past to fabricate hybrid materials that display the complementary properties of the polyester polycaprolactone (PCL) and the polysaccharide chitosan (CHS) for application in the field of bone regeneration and tissue engineering.
24998774	3	6	theme	subsequent	532:541	arg1	characterization					543:558	its subsequent characterization	528:558	its subsequent characterization	528:558	Here we report the synthesis of a chitosan-graft-polycaprolactone (CHS-g-PCL) and its subsequent characterization, including crystallinity, chemical structure and thermal stability.
24998774	5	7	from	cultivation	828:838	arg1	CHS-g-PCL					899:907	the surface-functionalized CHS-g-PCL	872:907	the surface-functionalized CHS-g-PCL	872:907	Finally, the cultivation of osteoblastic SaOS-2 cells on the surface-functionalized CHS-g-PCL was followed by analyses of cell viability, mineral deposition and alkaline phosphatase activity.
24998774	6	8	theme	osteogenic	1113:1122	arg1	activity					1124:1131	the osteogenic activity	1109:1131	the osteogenic activity of the silicatein/biosilica coating	1109:1167	These characterizations revealed a composite that combines the versatile properties of CHS-g-PCL with the osteogenic activity of the silicatein/biosilica coating and, hence, represents an innovative alternative to conventionally used CHS/PCL composites for biomedical applications, where stable bone-material interfaces are required.
24998774	4	9	theme	surface	765:771	arg1	morphology					773:782	surface morphology	765:782	surface morphology	765:782	Upon surface-functionalization of CHS-g-PCL with osteogenic biosilica via the surface-immobilized enzyme silicatein, protein adsorption, surface morphology and wettability were assessed.
24998774	3	10	theme	chemical	586:593	arg1	structure					595:603	chemical structure	586:603	chemical structure	586:603	Here we report the synthesis of a chitosan-graft-polycaprolactone (CHS-g-PCL) and its subsequent characterization, including crystallinity, chemical structure and thermal stability.
24998774	1	11	dep	Several	107:113	arg1	attempts					115:122	attempts	115:122	attempts	115:122	Several attempts have been made in the past to fabricate hybrid materials that display the complementary properties of the polyester polycaprolactone (PCL) and the polysaccharide chitosan (CHS) for application in the field of bone regeneration and tissue engineering.
24998774	5	12	theme	cell	937:940	arg1	viability					942:950	cell viability	937:950	cell viability	937:950	Finally, the cultivation of osteoblastic SaOS-2 cells on the surface-functionalized CHS-g-PCL was followed by analyses of cell viability, mineral deposition and alkaline phosphatase activity.
24998774	3	13	theme	chitosan-graft-polycaprolactone	480:510	arg1	synthesis					465:473	the synthesis	461:473	the synthesis of a chitosan-graft-polycaprolactone (CHS-g-PCL) and its subsequent characterization, including crystallinity, chemical structure and thermal stability	461:625	Here we report the synthesis of a chitosan-graft-polycaprolactone (CHS-g-PCL) and its subsequent characterization, including crystallinity, chemical structure and thermal stability.
24998774	1	14	theme	complementary	198:210	arg1	properties					212:221	the complementary properties	194:221	the complementary properties of the polyester polycaprolactone (PCL) and the polysaccharide chitosan (CHS) for application in the field of bone regeneration and tissue engineering	194:372	Several attempts have been made in the past to fabricate hybrid materials that display the complementary properties of the polyester polycaprolactone (PCL) and the polysaccharide chitosan (CHS) for application in the field of bone regeneration and tissue engineering.
24998774	6	15	theme	coating	1161:1167	arg1	activity					1124:1131	the osteogenic activity	1109:1131	the osteogenic activity of the silicatein/biosilica coating	1109:1167	These characterizations revealed a composite that combines the versatile properties of CHS-g-PCL with the osteogenic activity of the silicatein/biosilica coating and, hence, represents an innovative alternative to conventionally used CHS/PCL composites for biomedical applications, where stable bone-material interfaces are required.
24998774	3	16	theme	thermal	609:615	arg1	stability					617:625	thermal stability	609:625	thermal stability	609:625	Here we report the synthesis of a chitosan-graft-polycaprolactone (CHS-g-PCL) and its subsequent characterization, including crystallinity, chemical structure and thermal stability.
24998774	6	17	theme	innovative	1195:1204	arg1	alternative					1206:1216	an innovative alternative	1192:1216	an innovative alternative to conventionally used CHS/PCL composites for biomedical applications, where stable bone-material interfaces are required	1192:1338	These characterizations revealed a composite that combines the versatile properties of CHS-g-PCL with the osteogenic activity of the silicatein/biosilica coating and, hence, represents an innovative alternative to conventionally used CHS/PCL composites for biomedical applications, where stable bone-material interfaces are required.
24998774	1	18	from	application	305:315	arg1	field					324:328	the field	320:328	the field of bone regeneration and tissue engineering	320:372	Several attempts have been made in the past to fabricate hybrid materials that display the complementary properties of the polyester polycaprolactone (PCL) and the polysaccharide chitosan (CHS) for application in the field of bone regeneration and tissue engineering.
24998774	5	19	theme	mineral	953:959	arg1	deposition					961:970	mineral deposition	953:970	mineral deposition	953:970	Finally, the cultivation of osteoblastic SaOS-2 cells on the surface-functionalized CHS-g-PCL was followed by analyses of cell viability, mineral deposition and alkaline phosphatase activity.
24998774	5	19	theme	mineral	953:959	arg1	analyses					925:932	analyses	925:932	analyses of cell viability	925:950	Finally, the cultivation of osteoblastic SaOS-2 cells on the surface-functionalized CHS-g-PCL was followed by analyses of cell viability, mineral deposition and alkaline phosphatase activity.
24998774	6	20	theme	bone-material	1302:1314	arg1	interfaces					1316:1325	stable bone-material interfaces	1295:1325	stable bone-material interfaces	1295:1325	These characterizations revealed a composite that combines the versatile properties of CHS-g-PCL with the osteogenic activity of the silicatein/biosilica coating and, hence, represents an innovative alternative to conventionally used CHS/PCL composites for biomedical applications, where stable bone-material interfaces are required.
24998774	4	21	theme	protein	745:751	arg1	adsorption					753:762	protein adsorption	745:762	protein adsorption	745:762	Upon surface-functionalization of CHS-g-PCL with osteogenic biosilica via the surface-immobilized enzyme silicatein, protein adsorption, surface morphology and wettability were assessed.
24998774	5	22	theme	viability	942:950	arg1	deposition					961:970	mineral deposition	953:970	mineral deposition	953:970	Finally, the cultivation of osteoblastic SaOS-2 cells on the surface-functionalized CHS-g-PCL was followed by analyses of cell viability, mineral deposition and alkaline phosphatase activity.
24998774	5	22	theme	viability	942:950	arg1	activity					997:1004	alkaline phosphatase activity	976:1004	alkaline phosphatase activity	976:1004	Finally, the cultivation of osteoblastic SaOS-2 cells on the surface-functionalized CHS-g-PCL was followed by analyses of cell viability, mineral deposition and alkaline phosphatase activity.
24998774	5	22	theme	viability	942:950	arg1	analyses					925:932	analyses	925:932	analyses of cell viability	925:950	Finally, the cultivation of osteoblastic SaOS-2 cells on the surface-functionalized CHS-g-PCL was followed by analyses of cell viability, mineral deposition and alkaline phosphatase activity.
24998774	6	23	theme	CHS-g-PCL	1094:1102	arg1	properties					1080:1089	the versatile properties	1066:1089	the versatile properties of CHS-g-PCL	1066:1102	These characterizations revealed a composite that combines the versatile properties of CHS-g-PCL with the osteogenic activity of the silicatein/biosilica coating and, hence, represents an innovative alternative to conventionally used CHS/PCL composites for biomedical applications, where stable bone-material interfaces are required.
24998774	6	24	theme	stable	1295:1300	arg1	interfaces					1316:1325	stable bone-material interfaces	1295:1325	stable bone-material interfaces	1295:1325	These characterizations revealed a composite that combines the versatile properties of CHS-g-PCL with the osteogenic activity of the silicatein/biosilica coating and, hence, represents an innovative alternative to conventionally used CHS/PCL composites for biomedical applications, where stable bone-material interfaces are required.
24998774	1	25	theme	polyester	230:238	arg1	polycaprolactone					240:255	the polyester polycaprolactone	226:255	the polyester polycaprolactone (PCL)	226:261	Several attempts have been made in the past to fabricate hybrid materials that display the complementary properties of the polyester polycaprolactone (PCL) and the polysaccharide chitosan (CHS) for application in the field of bone regeneration and tissue engineering.
24998774	1	25	theme	polyester	230:238	arg1	PCL					258:260	PCL	258:260	PCL	258:260	Several attempts have been made in the past to fabricate hybrid materials that display the complementary properties of the polyester polycaprolactone (PCL) and the polysaccharide chitosan (CHS) for application in the field of bone regeneration and tissue engineering.
24998774	0	26	theme	osteogenic	21:30	arg1	activity					32:39	osteogenic activity	21:39	osteogenic activity	21:39	Characterization and osteogenic activity of a silicatein/biosilica-coated chitosan-graft-polycaprolactone.
24998774	6	27	dep	combines	1057:1064	arg1	represents					1181:1190	represents	1181:1190	represents an innovative alternative to conventionally used CHS/PCL composites for biomedical applications, where stable bone-material interfaces are required	1181:1338	These characterizations revealed a composite that combines the versatile properties of CHS-g-PCL with the osteogenic activity of the silicatein/biosilica coating and, hence, represents an innovative alternative to conventionally used CHS/PCL composites for biomedical applications, where stable bone-material interfaces are required.
24998774	1	28	theme	polycaprolactone	240:255	arg1	properties					212:221	the complementary properties	194:221	the complementary properties of the polyester polycaprolactone (PCL) and the polysaccharide chitosan (CHS) for application in the field of bone regeneration and tissue engineering	194:372	Several attempts have been made in the past to fabricate hybrid materials that display the complementary properties of the polyester polycaprolactone (PCL) and the polysaccharide chitosan (CHS) for application in the field of bone regeneration and tissue engineering.
24998774	2	29	contain	have	410:413	arg2	activity					429:436	no osteogenic activity	415:436	no osteogenic activity	415:436	However, such composites generally have no osteogenic activity per se.
24998774	2	29	contain	have	410:413	arg1	composites					389:398	such composites	384:398	such composites	384:398	However, such composites generally have no osteogenic activity per se.
24998774	5	30	theme	alkaline	976:983	arg1	phosphatase					985:995	alkaline phosphatase	976:995	alkaline phosphatase activity	976:1004	Finally, the cultivation of osteoblastic SaOS-2 cells on the surface-functionalized CHS-g-PCL was followed by analyses of cell viability, mineral deposition and alkaline phosphatase activity.
24998774	4	31	theme	enzyme	726:731	arg1	silicatein					733:742	the surface-immobilized enzyme silicatein	702:742	the surface-immobilized enzyme silicatein	702:742	Upon surface-functionalization of CHS-g-PCL with osteogenic biosilica via the surface-immobilized enzyme silicatein, protein adsorption, surface morphology and wettability were assessed.
24998774	2	32	theme	osteogenic	418:427	arg1	activity					429:436	no osteogenic activity	415:436	no osteogenic activity	415:436	However, such composites generally have no osteogenic activity per se.
24998774	6	33	theme	versatile	1070:1078	arg1	properties					1080:1089	the versatile properties	1066:1089	the versatile properties of CHS-g-PCL	1066:1102	These characterizations revealed a composite that combines the versatile properties of CHS-g-PCL with the osteogenic activity of the silicatein/biosilica coating and, hence, represents an innovative alternative to conventionally used CHS/PCL composites for biomedical applications, where stable bone-material interfaces are required.
24998774	4	34	theme	surface-immobilized	706:724	arg1	silicatein					733:742	the surface-immobilized enzyme silicatein	702:742	the surface-immobilized enzyme silicatein	702:742	Upon surface-functionalization of CHS-g-PCL with osteogenic biosilica via the surface-immobilized enzyme silicatein, protein adsorption, surface morphology and wettability were assessed.
24998774	0	35	theme	chitosan-graft-polycaprolactone	74:104	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and osteogenic activity of a silicatein/biosilica-coated chitosan-graft-polycaprolactone.
24998774	0	35	theme	chitosan-graft-polycaprolactone	74:104	arg1	activity					32:39	osteogenic activity	21:39	osteogenic activity	21:39	Characterization and osteogenic activity of a silicatein/biosilica-coated chitosan-graft-polycaprolactone.
24998774	5	36	theme	phosphatase	985:995	arg1	activity					997:1004	alkaline phosphatase activity	976:1004	alkaline phosphatase activity	976:1004	Finally, the cultivation of osteoblastic SaOS-2 cells on the surface-functionalized CHS-g-PCL was followed by analyses of cell viability, mineral deposition and alkaline phosphatase activity.
24998774	5	36	theme	phosphatase	985:995	arg1	analyses					925:932	analyses	925:932	analyses of cell viability	925:950	Finally, the cultivation of osteoblastic SaOS-2 cells on the surface-functionalized CHS-g-PCL was followed by analyses of cell viability, mineral deposition and alkaline phosphatase activity.
24998774	5	37	theme	osteoblastic	843:854	arg1	cells					863:867	osteoblastic SaOS-2 cells	843:867	osteoblastic SaOS-2 cells	843:867	Finally, the cultivation of osteoblastic SaOS-2 cells on the surface-functionalized CHS-g-PCL was followed by analyses of cell viability, mineral deposition and alkaline phosphatase activity.
24998774	0	38	theme	silicatein/biosilica-coated	46:72	arg1	chitosan-graft-polycaprolactone					74:104	a silicatein/biosilica-coated chitosan-graft-polycaprolactone	44:104	a silicatein/biosilica-coated chitosan-graft-polycaprolactone	44:104	Characterization and osteogenic activity of a silicatein/biosilica-coated chitosan-graft-polycaprolactone.
24998774	1	39	theme	bone	333:336	arg1	regeneration					338:349	bone regeneration	333:349	bone regeneration	333:349	Several attempts have been made in the past to fabricate hybrid materials that display the complementary properties of the polyester polycaprolactone (PCL) and the polysaccharide chitosan (CHS) for application in the field of bone regeneration and tissue engineering.
24998774	1	40	theme	regeneration	338:349	arg1	field					324:328	the field	320:328	the field of bone regeneration and tissue engineering	320:372	Several attempts have been made in the past to fabricate hybrid materials that display the complementary properties of the polyester polycaprolactone (PCL) and the polysaccharide chitosan (CHS) for application in the field of bone regeneration and tissue engineering.
24998774	6	41	theme	biomedical	1264:1273	arg1	applications					1275:1286	biomedical applications	1264:1286	biomedical applications	1264:1286	These characterizations revealed a composite that combines the versatile properties of CHS-g-PCL with the osteogenic activity of the silicatein/biosilica coating and, hence, represents an innovative alternative to conventionally used CHS/PCL composites for biomedical applications, where stable bone-material interfaces are required.
24998774	4	42	with	surface-functionalization	633:657	arg1	biosilica					688:696	osteogenic biosilica	677:696	osteogenic biosilica via the surface-immobilized enzyme silicatein	677:742	Upon surface-functionalization of CHS-g-PCL with osteogenic biosilica via the surface-immobilized enzyme silicatein, protein adsorption, surface morphology and wettability were assessed.
24998774	1	43	theme	hybrid	164:169	arg1	materials					171:179	hybrid materials	164:179	hybrid materials that display the complementary properties of the polyester polycaprolactone (PCL) and the polysaccharide chitosan (CHS) for application in the field of bone regeneration and tissue engineering	164:372	Several attempts have been made in the past to fabricate hybrid materials that display the complementary properties of the polyester polycaprolactone (PCL) and the polysaccharide chitosan (CHS) for application in the field of bone regeneration and tissue engineering.
24998774	4	44	theme	CHS-g-PCL	662:670	arg1	surface-functionalization					633:657	surface-functionalization	633:657	surface-functionalization of CHS-g-PCL with osteogenic biosilica via the surface-immobilized enzyme silicatein	633:742	Upon surface-functionalization of CHS-g-PCL with osteogenic biosilica via the surface-immobilized enzyme silicatein, protein adsorption, surface morphology and wettability were assessed.
24998774	6	45	theme	CHS/PCL	1241:1247	arg1	composites					1249:1258	conventionally used CHS/PCL composites	1221:1258	conventionally used CHS/PCL composites	1221:1258	These characterizations revealed a composite that combines the versatile properties of CHS-g-PCL with the osteogenic activity of the silicatein/biosilica coating and, hence, represents an innovative alternative to conventionally used CHS/PCL composites for biomedical applications, where stable bone-material interfaces are required.
24998774	3	46	theme	characterization	543:558	arg1	synthesis					465:473	the synthesis	461:473	the synthesis of a chitosan-graft-polycaprolactone (CHS-g-PCL) and its subsequent characterization, including crystallinity, chemical structure and thermal stability	461:625	Here we report the synthesis of a chitosan-graft-polycaprolactone (CHS-g-PCL) and its subsequent characterization, including crystallinity, chemical structure and thermal stability.
24998774	6	47	theme	silicatein/biosilica	1140:1159	arg1	coating					1161:1167	the silicatein/biosilica coating	1136:1167	the silicatein/biosilica coating	1136:1167	These characterizations revealed a composite that combines the versatile properties of CHS-g-PCL with the osteogenic activity of the silicatein/biosilica coating and, hence, represents an innovative alternative to conventionally used CHS/PCL composites for biomedical applications, where stable bone-material interfaces are required.
24998774	5	48	theme	surface-functionalized	876:897	arg1	CHS-g-PCL					899:907	the surface-functionalized CHS-g-PCL	872:907	the surface-functionalized CHS-g-PCL	872:907	Finally, the cultivation of osteoblastic SaOS-2 cells on the surface-functionalized CHS-g-PCL was followed by analyses of cell viability, mineral deposition and alkaline phosphatase activity.
24998774	4	49	theme	osteogenic	677:686	arg1	biosilica					688:696	osteogenic biosilica	677:696	osteogenic biosilica via the surface-immobilized enzyme silicatein	677:742	Upon surface-functionalization of CHS-g-PCL with osteogenic biosilica via the surface-immobilized enzyme silicatein, protein adsorption, surface morphology and wettability were assessed.
24998774	2	50	theme	such	384:387	arg1	composites					389:398	such composites	384:398	such composites	384:398	However, such composites generally have no osteogenic activity per se.
24998774	1	51	theme	polysaccharide	271:284	arg1	CHS					296:298	CHS	296:298	CHS	296:298	Several attempts have been made in the past to fabricate hybrid materials that display the complementary properties of the polyester polycaprolactone (PCL) and the polysaccharide chitosan (CHS) for application in the field of bone regeneration and tissue engineering.
24998774	1	51	theme	polysaccharide	271:284	arg1	chitosan					286:293	the polysaccharide chitosan	267:293	the polysaccharide chitosan (CHS) for application in the field of bone regeneration and tissue engineering	267:372	Several attempts have been made in the past to fabricate hybrid materials that display the complementary properties of the polyester polycaprolactone (PCL) and the polysaccharide chitosan (CHS) for application in the field of bone regeneration and tissue engineering.
29279978	4	0	from	hemicellulose	845:857	arg1	achievement					811:821	the achievement	807:821	the achievement of EB production from hemicellulose through CBP	807:869	Among these annotated proteins, xylanases, xylosidases, and bifunctional alcohol/aldehyde dehydrogenase (AdhE) play key roles in the achievement of EB production from hemicellulose through CBP.
29279978	4	0	from	hemicellulose	845:857	arg1	production					829:838	EB production	826:838	EB production from hemicellulose through CBP	826:869	Among these annotated proteins, xylanases, xylosidases, and bifunctional alcohol/aldehyde dehydrogenase (AdhE) play key roles in the achievement of EB production from hemicellulose through CBP.
29279978	1	1	theme	Thermoanaerobacterium	184:204	arg1	M5					228:229	A novel thermophilic and butanogenic Thermoanaerobacterium thermosaccharolyticum M5	147:229	A novel thermophilic and butanogenic Thermoanaerobacterium thermosaccharolyticum M5	147:229	A novel thermophilic and butanogenic Thermoanaerobacterium thermosaccharolyticum M5 was successfully isolated and characterized, which could produce butanol from hemicellulose via a unique ethanol-butanol (EB) pathway through consolidated bioprocessing (CBP).
29279978	1	2	theme	thermosaccharolyticum	206:226	arg1	M5					228:229	A novel thermophilic and butanogenic Thermoanaerobacterium thermosaccharolyticum M5	147:229	A novel thermophilic and butanogenic Thermoanaerobacterium thermosaccharolyticum M5	147:229	A novel thermophilic and butanogenic Thermoanaerobacterium thermosaccharolyticum M5 was successfully isolated and characterized, which could produce butanol from hemicellulose via a unique ethanol-butanol (EB) pathway through consolidated bioprocessing (CBP).
29279978	4	3	theme	EB	826:827	arg1	production					829:838	EB production	826:838	EB production from hemicellulose through CBP	826:869	Among these annotated proteins, xylanases, xylosidases, and bifunctional alcohol/aldehyde dehydrogenase (AdhE) play key roles in the achievement of EB production from hemicellulose through CBP.
29279978	3	4	theme	M5	576:577	arg1	2.64 Mp					582:588	2.64 Mp	582:588	2.64 Mp	582:588	The assembled draft genome of strain M5 is 2.64 Mp, which contains 2638 genes and 2465 protein-coding sequences with 33.90% G + C content.
29279978	3	4	theme	M5	576:577	arg1	genome					559:564	The assembled draft genome	539:564	The assembled draft genome of strain M5	539:577	The assembled draft genome of strain M5 is 2.64 Mp, which contains 2638 genes and 2465 protein-coding sequences with 33.90% G + C content.
29279978	1	5	theme	unique	329:334	arg1	pathway					357:363	a unique ethanol-butanol (EB) pathway	327:363	a unique ethanol-butanol (EB) pathway through consolidated bioprocessing (CBP)	327:404	A novel thermophilic and butanogenic Thermoanaerobacterium thermosaccharolyticum M5 was successfully isolated and characterized, which could produce butanol from hemicellulose via a unique ethanol-butanol (EB) pathway through consolidated bioprocessing (CBP).
29279978	4	6	theme	annotated	690:698	arg1	proteins					700:707	these annotated proteins	684:707	these annotated proteins	684:707	Among these annotated proteins, xylanases, xylosidases, and bifunctional alcohol/aldehyde dehydrogenase (AdhE) play key roles in the achievement of EB production from hemicellulose through CBP.
29279978	3	7	with	genes	611:615	arg1	content					669:675	33.90% G + C content	656:675	33.90% G + C content	656:675	The assembled draft genome of strain M5 is 2.64 Mp, which contains 2638 genes and 2465 protein-coding sequences with 33.90% G + C content.
29279978	4	8	from	achievement	811:821	arg1	hemicellulose					845:857	hemicellulose	845:857	hemicellulose	845:857	Among these annotated proteins, xylanases, xylosidases, and bifunctional alcohol/aldehyde dehydrogenase (AdhE) play key roles in the achievement of EB production from hemicellulose through CBP.
29279978	4	9	theme	production	829:838	arg1	achievement					811:821	the achievement	807:821	the achievement of EB production from hemicellulose through CBP	807:869	Among these annotated proteins, xylanases, xylosidases, and bifunctional alcohol/aldehyde dehydrogenase (AdhE) play key roles in the achievement of EB production from hemicellulose through CBP.
29279978	2	10	theme	thermophilic	514:525	arg1	conditions					527:536	thermophilic conditions	514:536	thermophilic conditions	514:536	This represents the first wild-type bacterium which could produce butanol from hemicellulose via CBP under thermophilic conditions.
29279978	3	11	theme	protein-coding	626:639	arg1	sequences					641:649	2465 protein-coding sequences	621:649	2465 protein-coding sequences	621:649	The assembled draft genome of strain M5 is 2.64 Mp, which contains 2638 genes and 2465 protein-coding sequences with 33.90% G + C content.
29279978	1	12	theme	ethanol-butanol	336:350	arg1	pathway					357:363	a unique ethanol-butanol (EB) pathway	327:363	a unique ethanol-butanol (EB) pathway through consolidated bioprocessing (CBP)	327:404	A novel thermophilic and butanogenic Thermoanaerobacterium thermosaccharolyticum M5 was successfully isolated and characterized, which could produce butanol from hemicellulose via a unique ethanol-butanol (EB) pathway through consolidated bioprocessing (CBP).
29279978	2	13	theme	wild-type	433:441	arg1	bacterium					443:451	the first wild-type bacterium	423:451	the first wild-type bacterium which could produce butanol from hemicellulose via CBP under thermophilic conditions	423:536	This represents the first wild-type bacterium which could produce butanol from hemicellulose via CBP under thermophilic conditions.
29279978	3	14	theme	33.90	656:660	arg1	%					661:661	%	661:661	%	661:661	The assembled draft genome of strain M5 is 2.64 Mp, which contains 2638 genes and 2465 protein-coding sequences with 33.90% G + C content.
29279978	0	15	theme	Genome	10:15	arg1	Sequence					17:24	The Draft Genome Sequence	0:24	The Draft Genome Sequence of Thermophilic Thermoanaerobacterium thermosaccharolyticum M5 Capable of Directly Producing Butanol from Hemicellulose.	0:145	The Draft Genome Sequence of Thermophilic Thermoanaerobacterium thermosaccharolyticum M5 Capable of Directly Producing Butanol from Hemicellulose.
29279978	1	16	theme	EB	353:354	arg1	pathway					357:363	a unique ethanol-butanol (EB) pathway	327:363	a unique ethanol-butanol (EB) pathway through consolidated bioprocessing (CBP)	327:404	A novel thermophilic and butanogenic Thermoanaerobacterium thermosaccharolyticum M5 was successfully isolated and characterized, which could produce butanol from hemicellulose via a unique ethanol-butanol (EB) pathway through consolidated bioprocessing (CBP).
29279978	3	17	theme	assembled	543:551	arg1	2.64 Mp					582:588	2.64 Mp	582:588	2.64 Mp	582:588	The assembled draft genome of strain M5 is 2.64 Mp, which contains 2638 genes and 2465 protein-coding sequences with 33.90% G + C content.
29279978	3	17	theme	assembled	543:551	arg1	genome					559:564	The assembled draft genome	539:564	The assembled draft genome of strain M5	539:577	The assembled draft genome of strain M5 is 2.64 Mp, which contains 2638 genes and 2465 protein-coding sequences with 33.90% G + C content.
29279978	0	18	theme	Thermophilic	29:40	arg1	M5					86:87	Thermophilic Thermoanaerobacterium thermosaccharolyticum M5	29:87	Thermophilic Thermoanaerobacterium thermosaccharolyticum M5 Capable of Directly Producing Butanol from Hemicellulose	29:144	The Draft Genome Sequence of Thermophilic Thermoanaerobacterium thermosaccharolyticum M5 Capable of Directly Producing Butanol from Hemicellulose.
29279978	1	19	from	hemicellulose	309:321	arg1	butanol					296:302	butanol	296:302	butanol from hemicellulose	296:321	A novel thermophilic and butanogenic Thermoanaerobacterium thermosaccharolyticum M5 was successfully isolated and characterized, which could produce butanol from hemicellulose via a unique ethanol-butanol (EB) pathway through consolidated bioprocessing (CBP).
29279978	3	20	theme	%	661:661	arg1	content					669:675	33.90% G + C content	656:675	33.90% G + C content	656:675	The assembled draft genome of strain M5 is 2.64 Mp, which contains 2638 genes and 2465 protein-coding sequences with 33.90% G + C content.
29279978	4	21	theme	alcohol/aldehyde	751:766	arg1	dehydrogenase					768:780	bifunctional alcohol/aldehyde dehydrogenase	738:780	bifunctional alcohol/aldehyde dehydrogenase (AdhE)	738:787	Among these annotated proteins, xylanases, xylosidases, and bifunctional alcohol/aldehyde dehydrogenase (AdhE) play key roles in the achievement of EB production from hemicellulose through CBP.
29279978	4	21	theme	alcohol/aldehyde	751:766	arg1	AdhE					783:786	AdhE	783:786	AdhE	783:786	Among these annotated proteins, xylanases, xylosidases, and bifunctional alcohol/aldehyde dehydrogenase (AdhE) play key roles in the achievement of EB production from hemicellulose through CBP.
29279978	3	22	contain	contains	597:604	arg2	sequences					641:649	2465 protein-coding sequences	621:649	2465 protein-coding sequences	621:649	The assembled draft genome of strain M5 is 2.64 Mp, which contains 2638 genes and 2465 protein-coding sequences with 33.90% G + C content.
29279978	3	22	contain	contains	597:604	arg2	genes					611:615	2638 genes	606:615	2638 genes	606:615	The assembled draft genome of strain M5 is 2.64 Mp, which contains 2638 genes and 2465 protein-coding sequences with 33.90% G + C content.
29279978	3	22	contain	contains	597:604	arg1	2.64 Mp					582:588	2.64 Mp	582:588	2.64 Mp	582:588	The assembled draft genome of strain M5 is 2.64 Mp, which contains 2638 genes and 2465 protein-coding sequences with 33.90% G + C content.
29279978	3	22	contain	contains	597:604	arg1	genome					559:564	The assembled draft genome	539:564	The assembled draft genome of strain M5	539:577	The assembled draft genome of strain M5 is 2.64 Mp, which contains 2638 genes and 2465 protein-coding sequences with 33.90% G + C content.
29279978	3	23	theme	G + C	663:667	arg1	content					669:675	33.90% G + C content	656:675	33.90% G + C content	656:675	The assembled draft genome of strain M5 is 2.64 Mp, which contains 2638 genes and 2465 protein-coding sequences with 33.90% G + C content.
29279978	0	24	theme	thermosaccharolyticum	64:84	arg1	M5					86:87	Thermophilic Thermoanaerobacterium thermosaccharolyticum M5	29:87	Thermophilic Thermoanaerobacterium thermosaccharolyticum M5 Capable of Directly Producing Butanol from Hemicellulose	29:144	The Draft Genome Sequence of Thermophilic Thermoanaerobacterium thermosaccharolyticum M5 Capable of Directly Producing Butanol from Hemicellulose.
29279978	0	25	theme	Thermoanaerobacterium	42:62	arg1	M5					86:87	Thermophilic Thermoanaerobacterium thermosaccharolyticum M5	29:87	Thermophilic Thermoanaerobacterium thermosaccharolyticum M5 Capable of Directly Producing Butanol from Hemicellulose	29:144	The Draft Genome Sequence of Thermophilic Thermoanaerobacterium thermosaccharolyticum M5 Capable of Directly Producing Butanol from Hemicellulose.
29279978	1	26	theme	consolidated	373:384	arg1	CBP					401:403	CBP	401:403	CBP	401:403	A novel thermophilic and butanogenic Thermoanaerobacterium thermosaccharolyticum M5 was successfully isolated and characterized, which could produce butanol from hemicellulose via a unique ethanol-butanol (EB) pathway through consolidated bioprocessing (CBP).
29279978	1	26	theme	consolidated	373:384	arg1	bioprocessing					386:398	consolidated bioprocessing	373:398	consolidated bioprocessing (CBP)	373:404	A novel thermophilic and butanogenic Thermoanaerobacterium thermosaccharolyticum M5 was successfully isolated and characterized, which could produce butanol from hemicellulose via a unique ethanol-butanol (EB) pathway through consolidated bioprocessing (CBP).
29279978	1	27	theme	butanogenic	172:182	arg1	M5					228:229	A novel thermophilic and butanogenic Thermoanaerobacterium thermosaccharolyticum M5	147:229	A novel thermophilic and butanogenic Thermoanaerobacterium thermosaccharolyticum M5	147:229	A novel thermophilic and butanogenic Thermoanaerobacterium thermosaccharolyticum M5 was successfully isolated and characterized, which could produce butanol from hemicellulose via a unique ethanol-butanol (EB) pathway through consolidated bioprocessing (CBP).
29279978	3	28	theme	strain	569:574	arg1	M5					576:577	strain M5	569:577	strain M5	569:577	The assembled draft genome of strain M5 is 2.64 Mp, which contains 2638 genes and 2465 protein-coding sequences with 33.90% G + C content.
29279978	4	29	theme	bifunctional	738:749	arg1	dehydrogenase					768:780	bifunctional alcohol/aldehyde dehydrogenase	738:780	bifunctional alcohol/aldehyde dehydrogenase (AdhE)	738:787	Among these annotated proteins, xylanases, xylosidases, and bifunctional alcohol/aldehyde dehydrogenase (AdhE) play key roles in the achievement of EB production from hemicellulose through CBP.
29279978	4	29	theme	bifunctional	738:749	arg1	AdhE					783:786	AdhE	783:786	AdhE	783:786	Among these annotated proteins, xylanases, xylosidases, and bifunctional alcohol/aldehyde dehydrogenase (AdhE) play key roles in the achievement of EB production from hemicellulose through CBP.
29279978	1	30	theme	novel	149:153	arg1	M5					228:229	A novel thermophilic and butanogenic Thermoanaerobacterium thermosaccharolyticum M5	147:229	A novel thermophilic and butanogenic Thermoanaerobacterium thermosaccharolyticum M5	147:229	A novel thermophilic and butanogenic Thermoanaerobacterium thermosaccharolyticum M5 was successfully isolated and characterized, which could produce butanol from hemicellulose via a unique ethanol-butanol (EB) pathway through consolidated bioprocessing (CBP).
29279978	4	31	theme	key	794:796	arg1	roles					798:802	key roles	794:802	key roles	794:802	Among these annotated proteins, xylanases, xylosidases, and bifunctional alcohol/aldehyde dehydrogenase (AdhE) play key roles in the achievement of EB production from hemicellulose through CBP.
29279978	1	32	theme	thermophilic	155:166	arg1	M5					228:229	A novel thermophilic and butanogenic Thermoanaerobacterium thermosaccharolyticum M5	147:229	A novel thermophilic and butanogenic Thermoanaerobacterium thermosaccharolyticum M5	147:229	A novel thermophilic and butanogenic Thermoanaerobacterium thermosaccharolyticum M5 was successfully isolated and characterized, which could produce butanol from hemicellulose via a unique ethanol-butanol (EB) pathway through consolidated bioprocessing (CBP).
29279978	3	33	theme	draft	553:557	arg1	2.64 Mp					582:588	2.64 Mp	582:588	2.64 Mp	582:588	The assembled draft genome of strain M5 is 2.64 Mp, which contains 2638 genes and 2465 protein-coding sequences with 33.90% G + C content.
29279978	3	33	theme	draft	553:557	arg1	genome					559:564	The assembled draft genome	539:564	The assembled draft genome of strain M5	539:577	The assembled draft genome of strain M5 is 2.64 Mp, which contains 2638 genes and 2465 protein-coding sequences with 33.90% G + C content.
29279978	0	34	theme	Capable	89:95	arg1	M5					86:87	Thermophilic Thermoanaerobacterium thermosaccharolyticum M5	29:87	Thermophilic Thermoanaerobacterium thermosaccharolyticum M5 Capable of Directly Producing Butanol from Hemicellulose	29:144	The Draft Genome Sequence of Thermophilic Thermoanaerobacterium thermosaccharolyticum M5 Capable of Directly Producing Butanol from Hemicellulose.
29279978	2	35	theme	first	427:431	arg1	bacterium					443:451	the first wild-type bacterium	423:451	the first wild-type bacterium which could produce butanol from hemicellulose via CBP under thermophilic conditions	423:536	This represents the first wild-type bacterium which could produce butanol from hemicellulose via CBP under thermophilic conditions.
29279978	0	36	theme	M5	86:87	arg1	Sequence					17:24	The Draft Genome Sequence	0:24	The Draft Genome Sequence of Thermophilic Thermoanaerobacterium thermosaccharolyticum M5 Capable of Directly Producing Butanol from Hemicellulose.	0:145	The Draft Genome Sequence of Thermophilic Thermoanaerobacterium thermosaccharolyticum M5 Capable of Directly Producing Butanol from Hemicellulose.
29279978	3	37	with	sequences	641:649	arg1	content					669:675	33.90% G + C content	656:675	33.90% G + C content	656:675	The assembled draft genome of strain M5 is 2.64 Mp, which contains 2638 genes and 2465 protein-coding sequences with 33.90% G + C content.
29279978	2	38	from	hemicellulose	486:498	arg1	butanol					473:479	butanol	473:479	butanol from hemicellulose	473:498	This represents the first wild-type bacterium which could produce butanol from hemicellulose via CBP under thermophilic conditions.
29279978	0	39	theme	Draft	4:8	arg1	Sequence					17:24	The Draft Genome Sequence	0:24	The Draft Genome Sequence of Thermophilic Thermoanaerobacterium thermosaccharolyticum M5 Capable of Directly Producing Butanol from Hemicellulose.	0:145	The Draft Genome Sequence of Thermophilic Thermoanaerobacterium thermosaccharolyticum M5 Capable of Directly Producing Butanol from Hemicellulose.
27840213	2	0	theme	disaccharide	383:394	arg1	presence					359:366	the presence	355:366	the presence of nonsulfated disaccharide, monosulfated disaccharides ΔDi6S and ΔDi4S, and disulfated disaccharides in different percentages	355:493	The disaccharide composition produced by chondroitinase ABC treatment showed the presence of nonsulfated disaccharide, monosulfated disaccharides ΔDi6S and ΔDi4S, and disulfated disaccharides in different percentages.
27840213	3	1	from	%	571:571	arg1	particular					499:508	particular	499:508	particular	499:508	In particular, the nonsulfated disaccharide ΔDi0S of GTSG and SHSG were 3.5% and 5.5%, respectively, while monosulfated disaccharides ΔDi6S and ΔDi4S were evaluated to be 18.2%, 59% and 14.6%, 47.0%, respectively.
27840213	0	2	theme	sulfate	107:113	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification, structural characterization and antiproliferative properties of chondroitin sulfate/dermatan sulfate from tunisian fish skins.
27840213	0	2	theme	sulfate	107:113	arg1	characterization					25:40	structural characterization	14:40	structural characterization	14:40	Purification, structural characterization and antiproliferative properties of chondroitin sulfate/dermatan sulfate from tunisian fish skins.
27840213	0	2	theme	sulfate	107:113	arg1	properties					64:73	antiproliferative properties	46:73	antiproliferative properties	46:73	Purification, structural characterization and antiproliferative properties of chondroitin sulfate/dermatan sulfate from tunisian fish skins.
27840213	2	3	theme	nonsulfated	371:381	arg1	disaccharide					383:394	nonsulfated disaccharide	371:394	nonsulfated disaccharide	371:394	The disaccharide composition produced by chondroitinase ABC treatment showed the presence of nonsulfated disaccharide, monosulfated disaccharides ΔDi6S and ΔDi4S, and disulfated disaccharides in different percentages.
27840213	0	4	theme	fish	129:132	arg1	skins					134:138	tunisian fish skins	120:138	tunisian fish skins	120:138	Purification, structural characterization and antiproliferative properties of chondroitin sulfate/dermatan sulfate from tunisian fish skins.
27840213	3	5	theme	SHSG	558:561	arg1	ΔDi0S					540:544	the nonsulfated disaccharide ΔDi0S	511:544	the nonsulfated disaccharide ΔDi0S of GTSG and SHSG	511:561	In particular, the nonsulfated disaccharide ΔDi0S of GTSG and SHSG were 3.5% and 5.5%, respectively, while monosulfated disaccharides ΔDi6S and ΔDi4S were evaluated to be 18.2%, 59% and 14.6%, 47.0%, respectively.
27840213	3	5	theme	SHSG	558:561	arg1	%					571:571	3.5%	568:571	3.5%	568:571	In particular, the nonsulfated disaccharide ΔDi0S of GTSG and SHSG were 3.5% and 5.5%, respectively, while monosulfated disaccharides ΔDi6S and ΔDi4S were evaluated to be 18.2%, 59% and 14.6%, 47.0%, respectively.
27840213	5	6	theme	23.8KDa	939:945	arg1	values					916:921	average values	908:921	average values	908:921	PAGE analysis showed a GTSG and SHSG having molecular masses with average values of 41.72KDa and 23.8KDa, respectively.
27840213	6	7	theme	cell	969:972	arg1	proliferation					974:986	HCT116 cell proliferation	962:986	HCT116 cell proliferation	962:986	HCT116 cell proliferation was inhibited (p<0.05) by 70.6% and 72.65% at 200μg/mL of GTSG and SHSG respectively.
27840213	0	8	theme	tunisian	120:127	arg1	skins					134:138	tunisian fish skins	120:138	tunisian fish skins	120:138	Purification, structural characterization and antiproliferative properties of chondroitin sulfate/dermatan sulfate from tunisian fish skins.
27840213	2	9	attach	presence	359:366	arg2	disaccharides					456:468	disulfated disaccharides	445:468	disulfated disaccharides	445:468	The disaccharide composition produced by chondroitinase ABC treatment showed the presence of nonsulfated disaccharide, monosulfated disaccharides ΔDi6S and ΔDi4S, and disulfated disaccharides in different percentages.
27840213	2	9	attach	presence	359:366	arg1	percentages					483:493	different percentages	473:493	different percentages	473:493	The disaccharide composition produced by chondroitinase ABC treatment showed the presence of nonsulfated disaccharide, monosulfated disaccharides ΔDi6S and ΔDi4S, and disulfated disaccharides in different percentages.
27840213	2	9	attach	presence	359:366	arg2	ΔDi6S					424:428	ΔDi6S	424:428	ΔDi6S	424:428	The disaccharide composition produced by chondroitinase ABC treatment showed the presence of nonsulfated disaccharide, monosulfated disaccharides ΔDi6S and ΔDi4S, and disulfated disaccharides in different percentages.
27840213	2	9	attach	presence	359:366	arg2	disaccharides					410:422	monosulfated disaccharides ΔDi6S and ΔDi4S	397:438	monosulfated disaccharides ΔDi6S and ΔDi4S	397:438	The disaccharide composition produced by chondroitinase ABC treatment showed the presence of nonsulfated disaccharide, monosulfated disaccharides ΔDi6S and ΔDi4S, and disulfated disaccharides in different percentages.
27840213	2	9	attach	presence	359:366	arg2	disaccharide					383:394	nonsulfated disaccharide	371:394	nonsulfated disaccharide	371:394	The disaccharide composition produced by chondroitinase ABC treatment showed the presence of nonsulfated disaccharide, monosulfated disaccharides ΔDi6S and ΔDi4S, and disulfated disaccharides in different percentages.
27840213	2	9	attach	presence	359:366	arg2	ΔDi4S					434:438	ΔDi4S	434:438	ΔDi4S	434:438	The disaccharide composition produced by chondroitinase ABC treatment showed the presence of nonsulfated disaccharide, monosulfated disaccharides ΔDi6S and ΔDi4S, and disulfated disaccharides in different percentages.
27840213	4	10	theme	GTSG	747:750	arg1	analysis					735:742	Capillary elecrophoresis analysis	710:742	Capillary elecrophoresis analysis of GTSG and SHSG	710:759	Capillary elecrophoresis analysis of GTSG and SHSG contained 99.2% and 95.4% of chondroitin sulfate/dermatan sulfate, respectively.
27840213	3	11	theme	GTSG	549:552	arg1	ΔDi0S					540:544	the nonsulfated disaccharide ΔDi0S	511:544	the nonsulfated disaccharide ΔDi0S of GTSG and SHSG	511:561	In particular, the nonsulfated disaccharide ΔDi0S of GTSG and SHSG were 3.5% and 5.5%, respectively, while monosulfated disaccharides ΔDi6S and ΔDi4S were evaluated to be 18.2%, 59% and 14.6%, 47.0%, respectively.
27840213	3	11	theme	GTSG	549:552	arg1	%					571:571	3.5%	568:571	3.5%	568:571	In particular, the nonsulfated disaccharide ΔDi0S of GTSG and SHSG were 3.5% and 5.5%, respectively, while monosulfated disaccharides ΔDi6S and ΔDi4S were evaluated to be 18.2%, 59% and 14.6%, 47.0%, respectively.
27840213	5	12	theme	average	908:914	arg1	values					916:921	average values	908:921	average values	908:921	PAGE analysis showed a GTSG and SHSG having molecular masses with average values of 41.72KDa and 23.8KDa, respectively.
27840213	5	13	theme	molecular	886:894	arg1	masses					896:901	molecular masses	886:901	molecular masses	886:901	PAGE analysis showed a GTSG and SHSG having molecular masses with average values of 41.72KDa and 23.8KDa, respectively.
27840213	6	14	theme	GTSG	1046:1049	arg1	200μg/mL					1034:1041	200μg/mL	1034:1041	200μg/mL of GTSG and SHSG respectively	1034:1071	HCT116 cell proliferation was inhibited (p<0.05) by 70.6% and 72.65% at 200μg/mL of GTSG and SHSG respectively.
27840213	2	15	theme	disulfated	445:454	arg1	disaccharides					456:468	disulfated disaccharides	445:468	disulfated disaccharides	445:468	The disaccharide composition produced by chondroitinase ABC treatment showed the presence of nonsulfated disaccharide, monosulfated disaccharides ΔDi6S and ΔDi4S, and disulfated disaccharides in different percentages.
27840213	2	16	theme	disaccharide	282:293	arg1	composition					295:305	The disaccharide composition	278:305	The disaccharide composition produced by chondroitinase ABC treatment	278:346	The disaccharide composition produced by chondroitinase ABC treatment showed the presence of nonsulfated disaccharide, monosulfated disaccharides ΔDi6S and ΔDi4S, and disulfated disaccharides in different percentages.
27840213	5	17	theme	PAGE	842:845	arg1	analysis					847:854	PAGE analysis	842:854	PAGE analysis	842:854	PAGE analysis showed a GTSG and SHSG having molecular masses with average values of 41.72KDa and 23.8KDa, respectively.
27840213	6	18	theme	HCT116	962:967	arg1	proliferation					974:986	HCT116 cell proliferation	962:986	HCT116 cell proliferation	962:986	HCT116 cell proliferation was inhibited (p<0.05) by 70.6% and 72.65% at 200μg/mL of GTSG and SHSG respectively.
27840213	4	19	theme	sulfate	819:825	arg1	sulfate					819:825	chondroitin sulfate/dermatan sulfate	790:825	chondroitin sulfate/dermatan sulfate	790:825	Capillary elecrophoresis analysis of GTSG and SHSG contained 99.2% and 95.4% of chondroitin sulfate/dermatan sulfate, respectively.
27840213	4	19	theme	sulfate	819:825	arg1	%					775:775	99.2%	771:775	99.2%	771:775	Capillary elecrophoresis analysis of GTSG and SHSG contained 99.2% and 95.4% of chondroitin sulfate/dermatan sulfate, respectively.
27840213	4	19	theme	sulfate	819:825	arg1	%					785:785	95.4%	781:785	95.4% of chondroitin sulfate/dermatan sulfate	781:825	Capillary elecrophoresis analysis of GTSG and SHSG contained 99.2% and 95.4% of chondroitin sulfate/dermatan sulfate, respectively.
27840213	4	20	theme	SHSG	756:759	arg1	analysis					735:742	Capillary elecrophoresis analysis	710:742	Capillary elecrophoresis analysis of GTSG and SHSG	710:759	Capillary elecrophoresis analysis of GTSG and SHSG contained 99.2% and 95.4% of chondroitin sulfate/dermatan sulfate, respectively.
27840213	7	21	theme	novel	1168:1172	arg1	potential					1134:1142	promising antiproliferative potential	1106:1142	promising antiproliferative potential	1106:1142	Both GTSG and SHSG demonstrated promising antiproliferative potential, which may be used as a novel, effective agent.
27840213	7	21	theme	novel	1168:1172	arg1	agent					1185:1189	a novel, effective agent	1166:1189	a novel, effective agent	1166:1189	Both GTSG and SHSG demonstrated promising antiproliferative potential, which may be used as a novel, effective agent.
27840213	4	22	theme	elecrophoresis	720:733	arg1	analysis					735:742	Capillary elecrophoresis analysis	710:742	Capillary elecrophoresis analysis of GTSG and SHSG	710:759	Capillary elecrophoresis analysis of GTSG and SHSG contained 99.2% and 95.4% of chondroitin sulfate/dermatan sulfate, respectively.
27840213	6	23	dep	inhibited	992:1000	arg1	p<0.05					1003:1008	p<0.05	1003:1008	p<0.05	1003:1008	HCT116 cell proliferation was inhibited (p<0.05) by 70.6% and 72.65% at 200μg/mL of GTSG and SHSG respectively.
27840213	3	24	dep	disaccharides	616:628	arg1	disaccharides					616:628	monosulfated disaccharides ΔDi6S and ΔDi4S	603:644	monosulfated disaccharides ΔDi6S and ΔDi4S	603:644	In particular, the nonsulfated disaccharide ΔDi0S of GTSG and SHSG were 3.5% and 5.5%, respectively, while monosulfated disaccharides ΔDi6S and ΔDi4S were evaluated to be 18.2%, 59% and 14.6%, 47.0%, respectively.
27840213	3	24	dep	disaccharides	616:628	arg1	ΔDi4S					640:644	ΔDi4S	640:644	ΔDi4S	640:644	In particular, the nonsulfated disaccharide ΔDi0S of GTSG and SHSG were 3.5% and 5.5%, respectively, while monosulfated disaccharides ΔDi6S and ΔDi4S were evaluated to be 18.2%, 59% and 14.6%, 47.0%, respectively.
27840213	3	24	dep	disaccharides	616:628	arg1	ΔDi6S					630:634	ΔDi6S	630:634	ΔDi6S	630:634	In particular, the nonsulfated disaccharide ΔDi0S of GTSG and SHSG were 3.5% and 5.5%, respectively, while monosulfated disaccharides ΔDi6S and ΔDi4S were evaluated to be 18.2%, 59% and 14.6%, 47.0%, respectively.
27840213	7	25	used	used	1158:1161	arg2	agent					1185:1189	a novel, effective agent	1166:1189	a novel, effective agent	1166:1189	Both GTSG and SHSG demonstrated promising antiproliferative potential, which may be used as a novel, effective agent.
27840213	7	25	used	used	1158:1161	arg2	potential					1134:1142	promising antiproliferative potential	1106:1142	promising antiproliferative potential	1106:1142	Both GTSG and SHSG demonstrated promising antiproliferative potential, which may be used as a novel, effective agent.
27840213	2	26	theme	ABC	334:336	arg1	treatment					338:346	chondroitinase ABC treatment	319:346	chondroitinase ABC treatment	319:346	The disaccharide composition produced by chondroitinase ABC treatment showed the presence of nonsulfated disaccharide, monosulfated disaccharides ΔDi6S and ΔDi4S, and disulfated disaccharides in different percentages.
27840213	2	27	theme	disaccharides	410:422	arg1	presence					359:366	the presence	355:366	the presence of nonsulfated disaccharide, monosulfated disaccharides ΔDi6S and ΔDi4S, and disulfated disaccharides in different percentages	355:493	The disaccharide composition produced by chondroitinase ABC treatment showed the presence of nonsulfated disaccharide, monosulfated disaccharides ΔDi6S and ΔDi4S, and disulfated disaccharides in different percentages.
27840213	2	28	theme	different	473:481	arg1	percentages					483:493	different percentages	473:493	different percentages	473:493	The disaccharide composition produced by chondroitinase ABC treatment showed the presence of nonsulfated disaccharide, monosulfated disaccharides ΔDi6S and ΔDi4S, and disulfated disaccharides in different percentages.
27840213	4	29	theme	Capillary	710:718	arg1	analysis					735:742	Capillary elecrophoresis analysis	710:742	Capillary elecrophoresis analysis of GTSG and SHSG	710:759	Capillary elecrophoresis analysis of GTSG and SHSG contained 99.2% and 95.4% of chondroitin sulfate/dermatan sulfate, respectively.
27840213	2	30	from	presence	359:366	arg1	percentages					483:493	different percentages	473:493	different percentages	473:493	The disaccharide composition produced by chondroitinase ABC treatment showed the presence of nonsulfated disaccharide, monosulfated disaccharides ΔDi6S and ΔDi4S, and disulfated disaccharides in different percentages.
27840213	2	31	theme	chondroitinase	319:332	arg1	ABC					334:336	chondroitinase ABC	319:336	chondroitinase ABC treatment	319:346	The disaccharide composition produced by chondroitinase ABC treatment showed the presence of nonsulfated disaccharide, monosulfated disaccharides ΔDi6S and ΔDi4S, and disulfated disaccharides in different percentages.
27840213	2	32	theme	disaccharides	456:468	arg1	presence					359:366	the presence	355:366	the presence of nonsulfated disaccharide, monosulfated disaccharides ΔDi6S and ΔDi4S, and disulfated disaccharides in different percentages	355:493	The disaccharide composition produced by chondroitinase ABC treatment showed the presence of nonsulfated disaccharide, monosulfated disaccharides ΔDi6S and ΔDi4S, and disulfated disaccharides in different percentages.
27840213	0	33	theme	structural	14:23	arg1	characterization					25:40	structural characterization	14:40	structural characterization	14:40	Purification, structural characterization and antiproliferative properties of chondroitin sulfate/dermatan sulfate from tunisian fish skins.
27840213	7	34	dep	novel	1168:1172	arg1	effective					1175:1183	effective	1175:1183	effective	1175:1183	Both GTSG and SHSG demonstrated promising antiproliferative potential, which may be used as a novel, effective agent.
27840213	1	35	theme	triggerfish	234:244	arg1	skins					220:224	the skins	216:224	the skins of grey triggerfish (GTSG) and smooth hound (SHSG)	216:275	Chondroitin sulfate/dermatan sulfate GAGs were extracted and purified from the skins of grey triggerfish (GTSG) and smooth hound (SHSG).
27840213	0	36	theme	antiproliferative	46:62	arg1	properties					64:73	antiproliferative properties	46:73	antiproliferative properties	46:73	Purification, structural characterization and antiproliferative properties of chondroitin sulfate/dermatan sulfate from tunisian fish skins.
27840213	7	37	theme	promising	1106:1114	arg1	potential					1134:1142	promising antiproliferative potential	1106:1142	promising antiproliferative potential	1106:1142	Both GTSG and SHSG demonstrated promising antiproliferative potential, which may be used as a novel, effective agent.
27840213	7	37	theme	promising	1106:1114	arg1	agent					1185:1189	a novel, effective agent	1166:1189	a novel, effective agent	1166:1189	Both GTSG and SHSG demonstrated promising antiproliferative potential, which may be used as a novel, effective agent.
27840213	7	38	theme	antiproliferative	1116:1132	arg1	potential					1134:1142	promising antiproliferative potential	1106:1142	promising antiproliferative potential	1106:1142	Both GTSG and SHSG demonstrated promising antiproliferative potential, which may be used as a novel, effective agent.
27840213	7	38	theme	antiproliferative	1116:1132	arg1	agent					1185:1189	a novel, effective agent	1166:1189	a novel, effective agent	1166:1189	Both GTSG and SHSG demonstrated promising antiproliferative potential, which may be used as a novel, effective agent.
27840213	4	39	contain	contained	761:769	arg1	analysis					735:742	Capillary elecrophoresis analysis	710:742	Capillary elecrophoresis analysis of GTSG and SHSG	710:759	Capillary elecrophoresis analysis of GTSG and SHSG contained 99.2% and 95.4% of chondroitin sulfate/dermatan sulfate, respectively.
27840213	4	39	contain	contained	761:769	arg2	sulfate					819:825	chondroitin sulfate/dermatan sulfate	790:825	chondroitin sulfate/dermatan sulfate	790:825	Capillary elecrophoresis analysis of GTSG and SHSG contained 99.2% and 95.4% of chondroitin sulfate/dermatan sulfate, respectively.
27840213	4	39	contain	contained	761:769	arg2	%					785:785	95.4%	781:785	95.4% of chondroitin sulfate/dermatan sulfate	781:825	Capillary elecrophoresis analysis of GTSG and SHSG contained 99.2% and 95.4% of chondroitin sulfate/dermatan sulfate, respectively.
27840213	4	39	contain	contained	761:769	arg2	%					775:775	99.2%	771:775	99.2%	771:775	Capillary elecrophoresis analysis of GTSG and SHSG contained 99.2% and 95.4% of chondroitin sulfate/dermatan sulfate, respectively.
27840213	4	40	theme	chondroitin	790:800	arg1	sulfate					819:825	chondroitin sulfate/dermatan sulfate	790:825	chondroitin sulfate/dermatan sulfate	790:825	Capillary elecrophoresis analysis of GTSG and SHSG contained 99.2% and 95.4% of chondroitin sulfate/dermatan sulfate, respectively.
27840213	4	41	theme	sulfate/dermatan	802:817	arg1	sulfate					819:825	chondroitin sulfate/dermatan sulfate	790:825	chondroitin sulfate/dermatan sulfate	790:825	Capillary elecrophoresis analysis of GTSG and SHSG contained 99.2% and 95.4% of chondroitin sulfate/dermatan sulfate, respectively.
27840213	6	42	theme	SHSG	1055:1058	arg1	200μg/mL					1034:1041	200μg/mL	1034:1041	200μg/mL of GTSG and SHSG respectively	1034:1071	HCT116 cell proliferation was inhibited (p<0.05) by 70.6% and 72.65% at 200μg/mL of GTSG and SHSG respectively.
27840213	1	43	theme	Chondroitin	141:151	arg1	GAGs					178:181	Chondroitin sulfate/dermatan sulfate GAGs	141:181	Chondroitin sulfate/dermatan sulfate GAGs	141:181	Chondroitin sulfate/dermatan sulfate GAGs were extracted and purified from the skins of grey triggerfish (GTSG) and smooth hound (SHSG).
27840213	3	44	theme	monosulfated	603:614	arg1	disaccharides					616:628	monosulfated disaccharides ΔDi6S and ΔDi4S	603:644	monosulfated disaccharides ΔDi6S and ΔDi4S	603:644	In particular, the nonsulfated disaccharide ΔDi0S of GTSG and SHSG were 3.5% and 5.5%, respectively, while monosulfated disaccharides ΔDi6S and ΔDi4S were evaluated to be 18.2%, 59% and 14.6%, 47.0%, respectively.
27840213	3	44	theme	monosulfated	603:614	arg1	ΔDi4S					640:644	ΔDi4S	640:644	ΔDi4S	640:644	In particular, the nonsulfated disaccharide ΔDi0S of GTSG and SHSG were 3.5% and 5.5%, respectively, while monosulfated disaccharides ΔDi6S and ΔDi4S were evaluated to be 18.2%, 59% and 14.6%, 47.0%, respectively.
27840213	3	44	theme	monosulfated	603:614	arg1	ΔDi6S					630:634	ΔDi6S	630:634	ΔDi6S	630:634	In particular, the nonsulfated disaccharide ΔDi0S of GTSG and SHSG were 3.5% and 5.5%, respectively, while monosulfated disaccharides ΔDi6S and ΔDi4S were evaluated to be 18.2%, 59% and 14.6%, 47.0%, respectively.
27840213	1	45	theme	sulfate/dermatan	153:168	arg1	GAGs					178:181	Chondroitin sulfate/dermatan sulfate GAGs	141:181	Chondroitin sulfate/dermatan sulfate GAGs	141:181	Chondroitin sulfate/dermatan sulfate GAGs were extracted and purified from the skins of grey triggerfish (GTSG) and smooth hound (SHSG).
27840213	3	46	from	%	580:580	arg1	particular					499:508	particular	499:508	particular	499:508	In particular, the nonsulfated disaccharide ΔDi0S of GTSG and SHSG were 3.5% and 5.5%, respectively, while monosulfated disaccharides ΔDi6S and ΔDi4S were evaluated to be 18.2%, 59% and 14.6%, 47.0%, respectively.
27840213	0	47	from	skins	134:138	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification, structural characterization and antiproliferative properties of chondroitin sulfate/dermatan sulfate from tunisian fish skins.
27840213	0	47	from	skins	134:138	arg1	characterization					25:40	structural characterization	14:40	structural characterization	14:40	Purification, structural characterization and antiproliferative properties of chondroitin sulfate/dermatan sulfate from tunisian fish skins.
27840213	0	47	from	skins	134:138	arg1	properties					64:73	antiproliferative properties	46:73	antiproliferative properties	46:73	Purification, structural characterization and antiproliferative properties of chondroitin sulfate/dermatan sulfate from tunisian fish skins.
27840213	1	48	theme	sulfate	170:176	arg1	GAGs					178:181	Chondroitin sulfate/dermatan sulfate GAGs	141:181	Chondroitin sulfate/dermatan sulfate GAGs	141:181	Chondroitin sulfate/dermatan sulfate GAGs were extracted and purified from the skins of grey triggerfish (GTSG) and smooth hound (SHSG).
27840213	5	49	theme	41.72KDa	926:933	arg1	values					916:921	average values	908:921	average values	908:921	PAGE analysis showed a GTSG and SHSG having molecular masses with average values of 41.72KDa and 23.8KDa, respectively.
27840213	2	50	dep	disaccharides	410:422	arg1	disaccharides					410:422	monosulfated disaccharides ΔDi6S and ΔDi4S	397:438	monosulfated disaccharides ΔDi6S and ΔDi4S	397:438	The disaccharide composition produced by chondroitinase ABC treatment showed the presence of nonsulfated disaccharide, monosulfated disaccharides ΔDi6S and ΔDi4S, and disulfated disaccharides in different percentages.
27840213	2	50	dep	disaccharides	410:422	arg1	ΔDi4S					434:438	ΔDi4S	434:438	ΔDi4S	434:438	The disaccharide composition produced by chondroitinase ABC treatment showed the presence of nonsulfated disaccharide, monosulfated disaccharides ΔDi6S and ΔDi4S, and disulfated disaccharides in different percentages.
27840213	2	50	dep	disaccharides	410:422	arg1	ΔDi6S					424:428	ΔDi6S	424:428	ΔDi6S	424:428	The disaccharide composition produced by chondroitinase ABC treatment showed the presence of nonsulfated disaccharide, monosulfated disaccharides ΔDi6S and ΔDi4S, and disulfated disaccharides in different percentages.
27840213	5	51	contain	having	879:884	arg1	GTSG					865:868	GTSG	865:868	GTSG	865:868	PAGE analysis showed a GTSG and SHSG having molecular masses with average values of 41.72KDa and 23.8KDa, respectively.
27840213	5	51	contain	having	879:884	arg2	masses					896:901	molecular masses	886:901	molecular masses	886:901	PAGE analysis showed a GTSG and SHSG having molecular masses with average values of 41.72KDa and 23.8KDa, respectively.
27840213	3	52	theme	disaccharide	527:538	arg1	ΔDi0S					540:544	the nonsulfated disaccharide ΔDi0S	511:544	the nonsulfated disaccharide ΔDi0S of GTSG and SHSG	511:561	In particular, the nonsulfated disaccharide ΔDi0S of GTSG and SHSG were 3.5% and 5.5%, respectively, while monosulfated disaccharides ΔDi6S and ΔDi4S were evaluated to be 18.2%, 59% and 14.6%, 47.0%, respectively.
27840213	3	52	theme	disaccharide	527:538	arg1	%					571:571	3.5%	568:571	3.5%	568:571	In particular, the nonsulfated disaccharide ΔDi0S of GTSG and SHSG were 3.5% and 5.5%, respectively, while monosulfated disaccharides ΔDi6S and ΔDi4S were evaluated to be 18.2%, 59% and 14.6%, 47.0%, respectively.
27840213	3	53	theme	nonsulfated	515:525	arg1	ΔDi0S					540:544	the nonsulfated disaccharide ΔDi0S	511:544	the nonsulfated disaccharide ΔDi0S of GTSG and SHSG	511:561	In particular, the nonsulfated disaccharide ΔDi0S of GTSG and SHSG were 3.5% and 5.5%, respectively, while monosulfated disaccharides ΔDi6S and ΔDi4S were evaluated to be 18.2%, 59% and 14.6%, 47.0%, respectively.
27840213	3	53	theme	nonsulfated	515:525	arg1	%					571:571	3.5%	568:571	3.5%	568:571	In particular, the nonsulfated disaccharide ΔDi0S of GTSG and SHSG were 3.5% and 5.5%, respectively, while monosulfated disaccharides ΔDi6S and ΔDi4S were evaluated to be 18.2%, 59% and 14.6%, 47.0%, respectively.
27840213	0	54	theme	sulfate/dermatan	90:105	arg1	sulfate					107:113	chondroitin sulfate/dermatan sulfate	78:113	chondroitin sulfate/dermatan sulfate	78:113	Purification, structural characterization and antiproliferative properties of chondroitin sulfate/dermatan sulfate from tunisian fish skins.
27840213	5	55	dep	GTSG	865:868	arg1	a					863:863	a	863:863	a	863:863	PAGE analysis showed a GTSG and SHSG having molecular masses with average values of 41.72KDa and 23.8KDa, respectively.
27840213	1	56	theme	grey	229:232	arg1	triggerfish					234:244	grey triggerfish	229:244	grey triggerfish (GTSG)	229:251	Chondroitin sulfate/dermatan sulfate GAGs were extracted and purified from the skins of grey triggerfish (GTSG) and smooth hound (SHSG).
27840213	1	56	theme	grey	229:232	arg1	GTSG					247:250	GTSG	247:250	GTSG	247:250	Chondroitin sulfate/dermatan sulfate GAGs were extracted and purified from the skins of grey triggerfish (GTSG) and smooth hound (SHSG).
27840213	2	57	theme	monosulfated	397:408	arg1	disaccharides					410:422	monosulfated disaccharides ΔDi6S and ΔDi4S	397:438	monosulfated disaccharides ΔDi6S and ΔDi4S	397:438	The disaccharide composition produced by chondroitinase ABC treatment showed the presence of nonsulfated disaccharide, monosulfated disaccharides ΔDi6S and ΔDi4S, and disulfated disaccharides in different percentages.
27840213	2	57	theme	monosulfated	397:408	arg1	ΔDi4S					434:438	ΔDi4S	434:438	ΔDi4S	434:438	The disaccharide composition produced by chondroitinase ABC treatment showed the presence of nonsulfated disaccharide, monosulfated disaccharides ΔDi6S and ΔDi4S, and disulfated disaccharides in different percentages.
27840213	2	57	theme	monosulfated	397:408	arg1	ΔDi6S					424:428	ΔDi6S	424:428	ΔDi6S	424:428	The disaccharide composition produced by chondroitinase ABC treatment showed the presence of nonsulfated disaccharide, monosulfated disaccharides ΔDi6S and ΔDi4S, and disulfated disaccharides in different percentages.
27840213	1	58	theme	smooth	257:262	arg1	SHSG					271:274	SHSG	271:274	SHSG	271:274	Chondroitin sulfate/dermatan sulfate GAGs were extracted and purified from the skins of grey triggerfish (GTSG) and smooth hound (SHSG).
27840213	1	58	theme	smooth	257:262	arg1	hound					264:268	smooth hound	257:268	smooth hound (SHSG)	257:275	Chondroitin sulfate/dermatan sulfate GAGs were extracted and purified from the skins of grey triggerfish (GTSG) and smooth hound (SHSG).
27840213	0	59	theme	chondroitin	78:88	arg1	sulfate					107:113	chondroitin sulfate/dermatan sulfate	78:113	chondroitin sulfate/dermatan sulfate	78:113	Purification, structural characterization and antiproliferative properties of chondroitin sulfate/dermatan sulfate from tunisian fish skins.
27840213	1	60	theme	hound	264:268	arg1	skins					220:224	the skins	216:224	the skins of grey triggerfish (GTSG) and smooth hound (SHSG)	216:275	Chondroitin sulfate/dermatan sulfate GAGs were extracted and purified from the skins of grey triggerfish (GTSG) and smooth hound (SHSG).
25563942	8	0	theme	stable	947:952	arg1	nanoparticles					968:980	highly stable electroactive nanoparticles	940:980	highly stable electroactive nanoparticles	940:980	Although the use of both polysaccharides (GA and GAD) produced highly stable electroactive nanoparticles, the best combination of properties was achieved for GA-PANI.
25563942	1	1	theme	chemical	230:237	arg1	synthesis					239:247	chemical synthesis	230:247	chemical synthesis	230:247	Electroactive nanoparticles combining gum arabic (GA) and polyaniline (PANI) were prepared by chemical synthesis.
25563942	5	2	theme	uronic	707:712	arg1	content					719:725	lower uronic acid content	701:725	lower uronic acid content	701:725	GAD has lower molecular mass, lower degree of branching and lower uronic acid content.
25563942	8	3	theme	polysaccharides	902:916	arg1	use					890:892	the use	886:892	the use of both polysaccharides (GA and GAD)	886:929	Although the use of both polysaccharides (GA and GAD) produced highly stable electroactive nanoparticles, the best combination of properties was achieved for GA-PANI.
25563942	1	4	theme	gum	174:176	arg1	GA					186:187	GA	186:187	GA	186:187	Electroactive nanoparticles combining gum arabic (GA) and polyaniline (PANI) were prepared by chemical synthesis.
25563942	1	4	theme	gum	174:176	arg1	arabic					178:183	gum arabic	174:183	gum arabic (GA)	174:188	Electroactive nanoparticles combining gum arabic (GA) and polyaniline (PANI) were prepared by chemical synthesis.
25563942	9	5	theme	hydrogen	1223:1230	arg1	bonds					1232:1236	hydrogen bonds	1223:1236	hydrogen bonds between the polymers	1223:1257	The sample GAD was not able to prevent the occurrence of crosslinking between PANI chains, possibly due to its lower microstructural complexity which diminishes the occurrence of hydrogen bonds between the polymers.
25563942	7	6	theme	obtained	803:810	arg1	composites					812:821	The obtained composites	799:821	The obtained composites (GA-PANI and GAD-PANI)	799:844	The obtained composites (GA-PANI and GAD-PANI) were thoroughly characterized.
25563942	7	6	theme	obtained	803:810	arg1	GAD-PANI					836:843	GAD-PANI	836:843	GAD-PANI	836:843	The obtained composites (GA-PANI and GAD-PANI) were thoroughly characterized.
25563942	7	6	theme	obtained	803:810	arg1	GA-PANI					824:830	GA-PANI	824:830	GA-PANI	824:830	The obtained composites (GA-PANI and GAD-PANI) were thoroughly characterized.
25563942	5	7	theme	lower	671:675	arg1	degree					677:682	lower degree	671:682	lower degree of branching	671:695	GAD has lower molecular mass, lower degree of branching and lower uronic acid content.
25563942	9	8	theme	bonds	1232:1236	arg1	occurrence					1209:1218	the occurrence	1205:1218	the occurrence of hydrogen bonds between the polymers	1205:1257	The sample GAD was not able to prevent the occurrence of crosslinking between PANI chains, possibly due to its lower microstructural complexity which diminishes the occurrence of hydrogen bonds between the polymers.
25563942	8	9	theme	electroactive	954:966	arg1	nanoparticles					968:980	highly stable electroactive nanoparticles	940:980	highly stable electroactive nanoparticles	940:980	Although the use of both polysaccharides (GA and GAD) produced highly stable electroactive nanoparticles, the best combination of properties was achieved for GA-PANI.
25563942	9	10	theme	crosslinking	1101:1112	arg1	occurrence					1087:1096	the occurrence	1083:1096	the occurrence of crosslinking between PANI chains	1083:1132	The sample GAD was not able to prevent the occurrence of crosslinking between PANI chains, possibly due to its lower microstructural complexity which diminishes the occurrence of hydrogen bonds between the polymers.
25563942	2	11	with	polysaccharides	294:308	arg1	content					328:334	some protein content	315:334	some protein content	315:334	The gum consists of highly branched anionic polysaccharides with some protein content.
25563942	4	12	theme	C	609:609	arg1	NMR					611:613	(13)C NMR	605:613	(13)C NMR	605:613	The modification was studied by SEC, GC-MS, (13)C NMR and colorimetric methods.
25563942	0	13	theme	microstructure	79:92	arg1	effect					59:64	the effect	55:64	the effect of the gum's microstructure in obtaining electroactive nanoparticles	55:133	Native and structurally modified gum arabic: exploring the effect of the gum's microstructure in obtaining electroactive nanoparticles.
25563942	9	14	theme	PANI	1122:1125	arg1	chains					1127:1132	PANI chains	1122:1132	PANI chains	1122:1132	The sample GAD was not able to prevent the occurrence of crosslinking between PANI chains, possibly due to its lower microstructural complexity which diminishes the occurrence of hydrogen bonds between the polymers.
25563942	3	15	theme	controlled	375:384	arg1	degradation					386:396	Smith controlled degradation	369:396	Smith controlled degradation	369:396	GA was structurally modified by Smith controlled degradation, in order to reduce its degree of branching (GAD), aiming the elucidation of the relationship between the structure and the properties of complex polysaccharides.
25563942	3	16	theme	complex	536:542	arg1	polysaccharides					544:558	complex polysaccharides	536:558	complex polysaccharides	536:558	GA was structurally modified by Smith controlled degradation, in order to reduce its degree of branching (GAD), aiming the elucidation of the relationship between the structure and the properties of complex polysaccharides.
25563942	0	17	theme	Native	0:5	arg1	arabic					37:42	Native and structurally modified gum arabic	0:42	Native and structurally modified gum arabic: exploring the effect of the gum's microstructure in obtaining electroactive nanoparticles.	0:134	Native and structurally modified gum arabic: exploring the effect of the gum's microstructure in obtaining electroactive nanoparticles.
25563942	8	18	theme	best	987:990	arg1	combination					992:1002	the best combination	983:1002	the best combination of properties	983:1016	Although the use of both polysaccharides (GA and GAD) produced highly stable electroactive nanoparticles, the best combination of properties was achieved for GA-PANI.
25563942	7	19	dep	composites	812:821	arg1	composites					812:821	The obtained composites	799:821	The obtained composites (GA-PANI and GAD-PANI)	799:844	The obtained composites (GA-PANI and GAD-PANI) were thoroughly characterized.
25563942	7	19	dep	composites	812:821	arg1	GAD-PANI					836:843	GAD-PANI	836:843	GAD-PANI	836:843	The obtained composites (GA-PANI and GAD-PANI) were thoroughly characterized.
25563942	7	19	dep	composites	812:821	arg1	GA-PANI					824:830	GA-PANI	824:830	GA-PANI	824:830	The obtained composites (GA-PANI and GAD-PANI) were thoroughly characterized.
25563942	3	20	theme	polysaccharides	544:558	arg1	structure					504:512	the structure	500:512	the structure	500:512	GA was structurally modified by Smith controlled degradation, in order to reduce its degree of branching (GAD), aiming the elucidation of the relationship between the structure and the properties of complex polysaccharides.
25563942	3	20	theme	polysaccharides	544:558	arg1	properties					522:531	the properties	518:531	the properties of complex polysaccharides	518:558	GA was structurally modified by Smith controlled degradation, in order to reduce its degree of branching (GAD), aiming the elucidation of the relationship between the structure and the properties of complex polysaccharides.
25563942	3	21	mod	modified	357:364	arg3	degradation					386:396	Smith controlled degradation	369:396	Smith controlled degradation	369:396	GA was structurally modified by Smith controlled degradation, in order to reduce its degree of branching (GAD), aiming the elucidation of the relationship between the structure and the properties of complex polysaccharides.
25563942	3	21	mod	modified	357:364	arg1	GA					337:338	GA	337:338	GA	337:338	GA was structurally modified by Smith controlled degradation, in order to reduce its degree of branching (GAD), aiming the elucidation of the relationship between the structure and the properties of complex polysaccharides.
25563942	0	22	theme	electroactive	107:119	arg1	nanoparticles					121:133	electroactive nanoparticles	107:133	electroactive nanoparticles	107:133	Native and structurally modified gum arabic: exploring the effect of the gum's microstructure in obtaining electroactive nanoparticles.
25563942	2	23	theme	anionic	286:292	arg1	polysaccharides					294:308	highly branched anionic polysaccharides	270:308	highly branched anionic polysaccharides with some protein content	270:334	The gum consists of highly branched anionic polysaccharides with some protein content.
25563942	3	24	theme	Smith	369:373	arg1	degradation					386:396	Smith controlled degradation	369:396	Smith controlled degradation	369:396	GA was structurally modified by Smith controlled degradation, in order to reduce its degree of branching (GAD), aiming the elucidation of the relationship between the structure and the properties of complex polysaccharides.
25563942	5	25	theme	lower	649:653	arg1	mass					665:668	lower molecular mass	649:668	lower molecular mass	649:668	GAD has lower molecular mass, lower degree of branching and lower uronic acid content.
25563942	2	26	theme	branched	277:284	arg1	polysaccharides					294:308	highly branched anionic polysaccharides	270:308	highly branched anionic polysaccharides with some protein content	270:334	The gum consists of highly branched anionic polysaccharides with some protein content.
25563942	8	27	dep	polysaccharides	902:916	arg1	GAD					926:928	GAD	926:928	GAD	926:928	Although the use of both polysaccharides (GA and GAD) produced highly stable electroactive nanoparticles, the best combination of properties was achieved for GA-PANI.
25563942	8	27	dep	polysaccharides	902:916	arg1	GA					919:920	GA	919:920	GA	919:920	Although the use of both polysaccharides (GA and GAD) produced highly stable electroactive nanoparticles, the best combination of properties was achieved for GA-PANI.
25563942	8	27	dep	polysaccharides	902:916	arg1	polysaccharides					902:916	both polysaccharides	897:916	both polysaccharides (GA and GAD)	897:929	Although the use of both polysaccharides (GA and GAD) produced highly stable electroactive nanoparticles, the best combination of properties was achieved for GA-PANI.
25563942	1	28	theme	Electroactive	136:148	arg1	nanoparticles					150:162	Electroactive nanoparticles	136:162	Electroactive nanoparticles combining gum arabic (GA) and polyaniline (PANI)	136:211	Electroactive nanoparticles combining gum arabic (GA) and polyaniline (PANI) were prepared by chemical synthesis.
25563942	5	29	theme	acid	714:717	arg1	content					719:725	lower uronic acid content	701:725	lower uronic acid content	701:725	GAD has lower molecular mass, lower degree of branching and lower uronic acid content.
25563942	5	30	contain	has	645:647	arg2	mass					665:668	lower molecular mass	649:668	lower molecular mass	649:668	GAD has lower molecular mass, lower degree of branching and lower uronic acid content.
25563942	5	30	contain	has	645:647	arg1	GAD					641:643	GAD	641:643	GAD	641:643	GAD has lower molecular mass, lower degree of branching and lower uronic acid content.
25563942	5	30	contain	has	645:647	arg2	content					719:725	lower uronic acid content	701:725	lower uronic acid content	701:725	GAD has lower molecular mass, lower degree of branching and lower uronic acid content.
25563942	5	30	contain	has	645:647	arg2	degree					677:682	lower degree	671:682	lower degree of branching	671:695	GAD has lower molecular mass, lower degree of branching and lower uronic acid content.
25563942	5	31	theme	molecular	655:663	arg1	mass					665:668	lower molecular mass	649:668	lower molecular mass	649:668	GAD has lower molecular mass, lower degree of branching and lower uronic acid content.
25563942	3	32	theme	branching	432:440	arg1	degree					422:427	its degree	418:427	its degree of branching (GAD)	418:446	GA was structurally modified by Smith controlled degradation, in order to reduce its degree of branching (GAD), aiming the elucidation of the relationship between the structure and the properties of complex polysaccharides.
25563942	0	33	theme	gum	33:35	arg1	arabic					37:42	Native and structurally modified gum arabic	0:42	Native and structurally modified gum arabic: exploring the effect of the gum's microstructure in obtaining electroactive nanoparticles.	0:134	Native and structurally modified gum arabic: exploring the effect of the gum's microstructure in obtaining electroactive nanoparticles.
25563942	0	34	theme	modified	24:31	arg1	arabic					37:42	Native and structurally modified gum arabic	0:42	Native and structurally modified gum arabic: exploring the effect of the gum's microstructure in obtaining electroactive nanoparticles.	0:134	Native and structurally modified gum arabic: exploring the effect of the gum's microstructure in obtaining electroactive nanoparticles.
25563942	9	35	theme	sample	1048:1053	arg1	GAD					1055:1057	The sample GAD	1044:1057	The sample GAD	1044:1057	The sample GAD was not able to prevent the occurrence of crosslinking between PANI chains, possibly due to its lower microstructural complexity which diminishes the occurrence of hydrogen bonds between the polymers.
25563942	9	35	theme	sample	1048:1053	arg1	able					1067:1070	able	1067:1070	able	1067:1070	The sample GAD was not able to prevent the occurrence of crosslinking between PANI chains, possibly due to its lower microstructural complexity which diminishes the occurrence of hydrogen bonds between the polymers.
25563942	0	36	dep	arabic	37:42	arg1	exploring					45:53	exploring	45:53	exploring the effect of the gum's microstructure in obtaining electroactive nanoparticles	45:133	Native and structurally modified gum arabic: exploring the effect of the gum's microstructure in obtaining electroactive nanoparticles.
25563942	8	37	theme	properties	1007:1016	arg1	combination					992:1002	the best combination	983:1002	the best combination of properties	983:1016	Although the use of both polysaccharides (GA and GAD) produced highly stable electroactive nanoparticles, the best combination of properties was achieved for GA-PANI.
25563942	9	38	theme	microstructural	1161:1175	arg1	complexity					1177:1186	its lower microstructural complexity	1151:1186	its lower microstructural complexity which diminishes the occurrence of hydrogen bonds between the polymers	1151:1257	The sample GAD was not able to prevent the occurrence of crosslinking between PANI chains, possibly due to its lower microstructural complexity which diminishes the occurrence of hydrogen bonds between the polymers.
25563942	2	39	theme	protein	320:326	arg1	content					328:334	some protein content	315:334	some protein content	315:334	The gum consists of highly branched anionic polysaccharides with some protein content.
25563942	5	40	theme	branching	687:695	arg1	degree					677:682	lower degree	671:682	lower degree of branching	671:695	GAD has lower molecular mass, lower degree of branching and lower uronic acid content.
25563942	5	40	theme	branching	687:695	arg1	content					719:725	lower uronic acid content	701:725	lower uronic acid content	701:725	GAD has lower molecular mass, lower degree of branching and lower uronic acid content.
25563942	5	40	theme	branching	687:695	arg1	mass					665:668	lower molecular mass	649:668	lower molecular mass	649:668	GAD has lower molecular mass, lower degree of branching and lower uronic acid content.
25563942	3	41	theme	relationship	479:490	arg1	elucidation					460:470	the elucidation	456:470	the elucidation of the relationship between the structure and the properties of complex polysaccharides	456:558	GA was structurally modified by Smith controlled degradation, in order to reduce its degree of branching (GAD), aiming the elucidation of the relationship between the structure and the properties of complex polysaccharides.
25563942	4	42	theme	colorimetric	619:630	arg1	methods					632:638	colorimetric methods	619:638	colorimetric methods	619:638	The modification was studied by SEC, GC-MS, (13)C NMR and colorimetric methods.
28744842	10	0	theme	nondestructive	1926:1939	arg1	monitoring					1941:1950	the nondestructive monitoring	1922:1950	the nondestructive monitoring of the structure and composition of the IVD at very high resolutions	1922:2019	Overall, this approach allows for the nondestructive monitoring of the structure and composition of the IVD at very high resolutions.
28744842	6	1	theme	magnetic	1267:1274	arg1	MRI					1295:1297	MRI	1295:1297	MRI	1295:1297	Moreover, we compared the imaging quality and quantitation of these in vitro models to magnetic resonance imaging (MRI) and histology.
28744842	6	1	theme	magnetic	1267:1274	arg1	imaging					1286:1292	magnetic resonance imaging	1267:1292	magnetic resonance imaging (MRI)	1267:1298	Moreover, we compared the imaging quality and quantitation of these in vitro models to magnetic resonance imaging (MRI) and histology.
28744842	7	2	theme	overtime	1464:1471	arg1	composition					1439:1449	composition	1439:1449	composition	1439:1449	Stab puncture, trypsin injection, and collagenase injection all induced detectable and significant changes in structure and composition of the discs overtime.
28744842	7	2	theme	overtime	1464:1471	arg1	structure					1425:1433	structure	1425:1433	structure	1425:1433	Stab puncture, trypsin injection, and collagenase injection all induced detectable and significant changes in structure and composition of the discs overtime.
28744842	4	3	theme	intact	876:881	arg1	IVD					898:900	the intact healthy murine IVD	872:900	the intact healthy murine IVD	872:900	We have previously demonstrated the feasibility of using Ioversol for the contrast-enhanced micro-computed tomography (microCT) to visualize and quantitate the intact healthy murine IVD.
28744842	8	4	from	progression	1586:1596	arg1	models					1607:1612	these models	1601:1612	these models	1601:1612	Compared to MRI and histology, contrast-enhanced microCT produced superior images that capture the degenerative progression in these models.
28744842	6	5	theme	models	1257:1262	arg1	quality					1214:1220	imaging quality	1206:1220	imaging quality	1206:1220	Moreover, we compared the imaging quality and quantitation of these in vitro models to magnetic resonance imaging (MRI) and histology.
28744842	6	5	theme	models	1257:1262	arg1	quantitation					1226:1237	quantitation	1226:1237	quantitation	1226:1237	Moreover, we compared the imaging quality and quantitation of these in vitro models to magnetic resonance imaging (MRI) and histology.
28744842	7	6	theme	significant	1402:1412	arg1	changes					1414:1420	detectable and significant changes	1387:1420	detectable and significant changes in structure and composition of the discs overtime	1387:1471	Stab puncture, trypsin injection, and collagenase injection all induced detectable and significant changes in structure and composition of the discs overtime.
28744842	6	7	theme	in	1248:1249	arg1	models					1257:1262	these in vitro models	1242:1262	these in vitro models	1242:1262	Moreover, we compared the imaging quality and quantitation of these in vitro models to magnetic resonance imaging (MRI) and histology.
28744842	7	8	theme	detectable	1387:1396	arg1	changes					1414:1420	detectable and significant changes	1387:1420	detectable and significant changes in structure and composition of the discs overtime	1387:1471	Stab puncture, trypsin injection, and collagenase injection all induced detectable and significant changes in structure and composition of the discs overtime.
28744842	5	9	dep	in	996:997	arg1	vitro					999:1003	vitro	999:1003	vitro	999:1003	In this work, we demonstrate utility of this approach to monitor the longitudinal changes of in vitro nucleolytic- and mechanical injury- degeneration models of the murine discs and introduce novel quantitative metrics to characterize the structure and composition of the IVD.
28744842	10	10	theme	IVD	1992:1994	arg1	composition					1973:1983	composition	1973:1983	composition	1973:1983	Overall, this approach allows for the nondestructive monitoring of the structure and composition of the IVD at very high resolutions.
28744842	10	10	theme	IVD	1992:1994	arg1	structure					1959:1967	structure	1959:1967	structure	1959:1967	Overall, this approach allows for the nondestructive monitoring of the structure and composition of the IVD at very high resolutions.
28744842	2	11	theme	mechanistic	331:341	arg1	changes					343:349	the mechanistic changes	327:349	the mechanistic changes in the development, aging, and diseased states of the IVD	327:407	Murine models are often used to investigate the mechanistic changes in the development, aging, and diseased states of the IVD, yet the detection of early degenerative changes in structure is challenging because of the minute size of the murine IVDs.
28744842	9	12	from	changes	1710:1716	arg1	height					1726:1731	disc height	1721:1731	disc height	1721:1731	Contrast-enhanced microCT was also capable of monitoring the structural deteriorations via the changes in disc height and volume, and novel the nucleus pulposus intensity/disc intensity (NI/DI) parameter provides a surrogate measure of proteoglycan composition (R = 0.96).
28744842	9	12	from	changes	1710:1716	arg1	volume					1737:1742	volume	1737:1742	volume	1737:1742	Contrast-enhanced microCT was also capable of monitoring the structural deteriorations via the changes in disc height and volume, and novel the nucleus pulposus intensity/disc intensity (NI/DI) parameter provides a surrogate measure of proteoglycan composition (R = 0.96).
28744842	0	13	theme	Micro-Computed	147:160	arg1	Tomography					162:171	Contrast-Enhanced Micro-Computed Tomography	129:171	Contrast-Enhanced Micro-Computed Tomography	129:171	The Quantitative Structural and Compositional Analyses of Degenerating Intervertebral Discs Using Magnetic Resonance Imaging and Contrast-Enhanced Micro-Computed Tomography.
28744842	7	14	theme	collagenase	1353:1363	arg1	injection					1365:1373	collagenase injection	1353:1373	collagenase injection	1353:1373	Stab puncture, trypsin injection, and collagenase injection all induced detectable and significant changes in structure and composition of the discs overtime.
28744842	5	15	theme	murine	1068:1073	arg1	discs					1075:1079	the murine discs	1064:1079	the murine discs	1064:1079	In this work, we demonstrate utility of this approach to monitor the longitudinal changes of in vitro nucleolytic- and mechanical injury- degeneration models of the murine discs and introduce novel quantitative metrics to characterize the structure and composition of the IVD.
28744842	9	16	theme	surrogate	1830:1838	arg1	measure					1840:1846	a surrogate measure	1828:1846	a surrogate measure of proteoglycan composition (R = 0.96)	1828:1885	Contrast-enhanced microCT was also capable of monitoring the structural deteriorations via the changes in disc height and volume, and novel the nucleus pulposus intensity/disc intensity (NI/DI) parameter provides a surrogate measure of proteoglycan composition (R = 0.96).
28744842	4	17	theme	murine	891:896	arg1	IVD					898:900	the intact healthy murine IVD	872:900	the intact healthy murine IVD	872:900	We have previously demonstrated the feasibility of using Ioversol for the contrast-enhanced micro-computed tomography (microCT) to visualize and quantitate the intact healthy murine IVD.
28744842	5	18	theme	mechanical	1022:1031	arg1	models					1054:1059	in vitro nucleolytic- and mechanical injury- degeneration models	996:1059	in vitro nucleolytic- and mechanical injury- degeneration models of the murine discs	996:1079	In this work, we demonstrate utility of this approach to monitor the longitudinal changes of in vitro nucleolytic- and mechanical injury- degeneration models of the murine discs and introduce novel quantitative metrics to characterize the structure and composition of the IVD.
28744842	7	19	theme	trypsin	1330:1336	arg1	injection					1338:1346	trypsin injection	1330:1346	trypsin injection	1330:1346	Stab puncture, trypsin injection, and collagenase injection all induced detectable and significant changes in structure and composition of the discs overtime.
28744842	5	20	theme	degeneration	1041:1052	arg1	models					1054:1059	in vitro nucleolytic- and mechanical injury- degeneration models	996:1059	in vitro nucleolytic- and mechanical injury- degeneration models of the murine discs	996:1079	In this work, we demonstrate utility of this approach to monitor the longitudinal changes of in vitro nucleolytic- and mechanical injury- degeneration models of the murine discs and introduce novel quantitative metrics to characterize the structure and composition of the IVD.
28744842	2	21	theme	Murine	283:288	arg1	models					290:295	Murine models	283:295	Murine models	283:295	Murine models are often used to investigate the mechanistic changes in the development, aging, and diseased states of the IVD, yet the detection of early degenerative changes in structure is challenging because of the minute size of the murine IVDs.
28744842	9	22	theme	nucleus	1759:1765	arg1	parameter					1809:1817	novel the nucleus pulposus intensity/disc intensity (NI/DI) parameter	1749:1817	novel the nucleus pulposus intensity/disc intensity (NI/DI) parameter	1749:1817	Contrast-enhanced microCT was also capable of monitoring the structural deteriorations via the changes in disc height and volume, and novel the nucleus pulposus intensity/disc intensity (NI/DI) parameter provides a surrogate measure of proteoglycan composition (R = 0.96).
28744842	6	23	dep	quality	1214:1220	arg1	the					1202:1204	the	1202:1204	the	1202:1204	Moreover, we compared the imaging quality and quantitation of these in vitro models to magnetic resonance imaging (MRI) and histology.
28744842	0	24	theme	Intervertebral	71:84	arg1	Discs					86:90	Degenerating Intervertebral Discs	58:90	Degenerating Intervertebral Discs	58:90	The Quantitative Structural and Compositional Analyses of Degenerating Intervertebral Discs Using Magnetic Resonance Imaging and Contrast-Enhanced Micro-Computed Tomography.
28744842	9	25	theme	intensity/disc	1776:1789	arg1	parameter					1809:1817	novel the nucleus pulposus intensity/disc intensity (NI/DI) parameter	1749:1817	novel the nucleus pulposus intensity/disc intensity (NI/DI) parameter	1749:1817	Contrast-enhanced microCT was also capable of monitoring the structural deteriorations via the changes in disc height and volume, and novel the nucleus pulposus intensity/disc intensity (NI/DI) parameter provides a surrogate measure of proteoglycan composition (R = 0.96).
28744842	10	26	from	composition	1973:1983	arg1	resolutions					2009:2019	very high resolutions	1999:2019	very high resolutions	1999:2019	Overall, this approach allows for the nondestructive monitoring of the structure and composition of the IVD at very high resolutions.
28744842	2	27	from	changes	343:349	arg1	development					358:368	development	358:368	development	358:368	Murine models are often used to investigate the mechanistic changes in the development, aging, and diseased states of the IVD, yet the detection of early degenerative changes in structure is challenging because of the minute size of the murine IVDs.
28744842	2	27	from	changes	343:349	arg1	states					391:396	diseased states	382:396	diseased states	382:396	Murine models are often used to investigate the mechanistic changes in the development, aging, and diseased states of the IVD, yet the detection of early degenerative changes in structure is challenging because of the minute size of the murine IVDs.
28744842	2	27	from	changes	343:349	arg1	aging					371:375	aging	371:375	aging	371:375	Murine models are often used to investigate the mechanistic changes in the development, aging, and diseased states of the IVD, yet the detection of early degenerative changes in structure is challenging because of the minute size of the murine IVDs.
28744842	2	28	theme	murine	520:525	arg1	IVDs					527:530	the murine IVDs	516:530	the murine IVDs	516:530	Murine models are often used to investigate the mechanistic changes in the development, aging, and diseased states of the IVD, yet the detection of early degenerative changes in structure is challenging because of the minute size of the murine IVDs.
28744842	0	29	theme	Resonance	107:115	arg1	Imaging					117:123	Magnetic Resonance Imaging	98:123	Magnetic Resonance Imaging	98:123	The Quantitative Structural and Compositional Analyses of Degenerating Intervertebral Discs Using Magnetic Resonance Imaging and Contrast-Enhanced Micro-Computed Tomography.
28744842	5	30	theme	approach	948:955	arg1	utility					932:938	utility	932:938	utility of this approach to monitor the longitudinal changes of in vitro nucleolytic- and mechanical injury- degeneration models of the murine discs and introduce novel quantitative metrics to characterize the structure and composition of the IVD	932:1177	In this work, we demonstrate utility of this approach to monitor the longitudinal changes of in vitro nucleolytic- and mechanical injury- degeneration models of the murine discs and introduce novel quantitative metrics to characterize the structure and composition of the IVD.
28744842	2	31	theme	IVD	405:407	arg1	development					358:368	development	358:368	development	358:368	Murine models are often used to investigate the mechanistic changes in the development, aging, and diseased states of the IVD, yet the detection of early degenerative changes in structure is challenging because of the minute size of the murine IVDs.
28744842	2	31	theme	IVD	405:407	arg1	states					391:396	diseased states	382:396	diseased states	382:396	Murine models are often used to investigate the mechanistic changes in the development, aging, and diseased states of the IVD, yet the detection of early degenerative changes in structure is challenging because of the minute size of the murine IVDs.
28744842	2	31	theme	IVD	405:407	arg1	aging					371:375	aging	371:375	aging	371:375	Murine models are often used to investigate the mechanistic changes in the development, aging, and diseased states of the IVD, yet the detection of early degenerative changes in structure is challenging because of the minute size of the murine IVDs.
28744842	5	32	theme	novel	1095:1099	arg1	metrics					1114:1120	novel quantitative metrics	1095:1120	novel quantitative metrics	1095:1120	In this work, we demonstrate utility of this approach to monitor the longitudinal changes of in vitro nucleolytic- and mechanical injury- degeneration models of the murine discs and introduce novel quantitative metrics to characterize the structure and composition of the IVD.
28744842	7	33	theme	Stab	1315:1318	arg1	puncture					1320:1327	Stab puncture	1315:1327	Stab puncture	1315:1327	Stab puncture, trypsin injection, and collagenase injection all induced detectable and significant changes in structure and composition of the discs overtime.
28744842	2	34	theme	changes	450:456	arg1	detection					418:426	the detection	414:426	the detection of early degenerative changes in structure	414:469	Murine models are often used to investigate the mechanistic changes in the development, aging, and diseased states of the IVD, yet the detection of early degenerative changes in structure is challenging because of the minute size of the murine IVDs.
28744842	2	35	theme	minute	501:506	arg1	size					508:511	the minute size	497:511	the minute size of the murine IVDs	497:530	Murine models are often used to investigate the mechanistic changes in the development, aging, and diseased states of the IVD, yet the detection of early degenerative changes in structure is challenging because of the minute size of the murine IVDs.
28744842	10	36	theme	structure	1959:1967	arg1	monitoring					1941:1950	the nondestructive monitoring	1922:1950	the nondestructive monitoring of the structure and composition of the IVD at very high resolutions	1922:2019	Overall, this approach allows for the nondestructive monitoring of the structure and composition of the IVD at very high resolutions.
28744842	2	37	theme	early	431:435	arg1	changes					450:456	early degenerative changes	431:456	early degenerative changes in structure	431:469	Murine models are often used to investigate the mechanistic changes in the development, aging, and diseased states of the IVD, yet the detection of early degenerative changes in structure is challenging because of the minute size of the murine IVDs.
28744842	8	38	theme	superior	1540:1547	arg1	images					1549:1554	superior images	1540:1554	superior images that capture the degenerative progression in these models	1540:1612	Compared to MRI and histology, contrast-enhanced microCT produced superior images that capture the degenerative progression in these models.
28744842	0	39	theme	Structural	17:26	arg1	Analyses					46:53	The Quantitative Structural and Compositional Analyses	0:53	The Quantitative Structural and Compositional Analyses of Degenerating Intervertebral Discs	0:90	The Quantitative Structural and Compositional Analyses of Degenerating Intervertebral Discs Using Magnetic Resonance Imaging and Contrast-Enhanced Micro-Computed Tomography.
28744842	10	40	dep	structure	1959:1967	arg1	the					1955:1957	the	1955:1957	the	1955:1957	Overall, this approach allows for the nondestructive monitoring of the structure and composition of the IVD at very high resolutions.
28744842	4	41	theme	micro-computed	808:821	arg1	microCT					835:841	microCT	835:841	microCT	835:841	We have previously demonstrated the feasibility of using Ioversol for the contrast-enhanced micro-computed tomography (microCT) to visualize and quantitate the intact healthy murine IVD.
28744842	4	41	theme	micro-computed	808:821	arg1	tomography					823:832	the contrast-enhanced micro-computed tomography	786:832	the contrast-enhanced micro-computed tomography (microCT)	786:842	We have previously demonstrated the feasibility of using Ioversol for the contrast-enhanced micro-computed tomography (microCT) to visualize and quantitate the intact healthy murine IVD.
28744842	0	42	theme	Compositional	32:44	arg1	Analyses					46:53	The Quantitative Structural and Compositional Analyses	0:53	The Quantitative Structural and Compositional Analyses of Degenerating Intervertebral Discs	0:90	The Quantitative Structural and Compositional Analyses of Degenerating Intervertebral Discs Using Magnetic Resonance Imaging and Contrast-Enhanced Micro-Computed Tomography.
28744842	9	43	theme	composition	1864:1874	arg1	measure					1840:1846	a surrogate measure	1828:1846	a surrogate measure of proteoglycan composition (R = 0.96)	1828:1885	Contrast-enhanced microCT was also capable of monitoring the structural deteriorations via the changes in disc height and volume, and novel the nucleus pulposus intensity/disc intensity (NI/DI) parameter provides a surrogate measure of proteoglycan composition (R = 0.96).
28744842	9	44	theme	Contrast-enhanced	1615:1631	arg1	microCT					1633:1639	Contrast-enhanced microCT	1615:1639	Contrast-enhanced microCT	1615:1639	Contrast-enhanced microCT was also capable of monitoring the structural deteriorations via the changes in disc height and volume, and novel the nucleus pulposus intensity/disc intensity (NI/DI) parameter provides a surrogate measure of proteoglycan composition (R = 0.96).
28744842	5	45	theme	longitudinal	972:983	arg1	changes					985:991	the longitudinal changes	968:991	the longitudinal changes of in vitro nucleolytic- and mechanical injury- degeneration models of the murine discs	968:1079	In this work, we demonstrate utility of this approach to monitor the longitudinal changes of in vitro nucleolytic- and mechanical injury- degeneration models of the murine discs and introduce novel quantitative metrics to characterize the structure and composition of the IVD.
28744842	3	46	theme	spatial	644:650	arg1	artifacts					633:641	sectioning artifacts	622:641	sectioning artifacts	622:641	Histology is the gold standard for examining the IVD structure, but it is susceptible to sectioning artifacts, spatial biases, and requires the destructive preparation of the sample.
28744842	3	46	theme	spatial	644:650	arg1	biases					652:657	spatial biases	644:657	spatial biases	644:657	Histology is the gold standard for examining the IVD structure, but it is susceptible to sectioning artifacts, spatial biases, and requires the destructive preparation of the sample.
28744842	2	47	theme	diseased	382:389	arg1	states					391:396	diseased states	382:396	diseased states	382:396	Murine models are often used to investigate the mechanistic changes in the development, aging, and diseased states of the IVD, yet the detection of early degenerative changes in structure is challenging because of the minute size of the murine IVDs.
28744842	1	48	theme	low	268:270	arg1	pain					277:280	low back pain	268:280	low back pain	268:280	The intervertebral disc (IVD) is susceptible to degenerative changes that are associated with low back pain.
28744842	2	49	from	detection	418:426	arg1	structure					461:469	structure	461:469	structure	461:469	Murine models are often used to investigate the mechanistic changes in the development, aging, and diseased states of the IVD, yet the detection of early degenerative changes in structure is challenging because of the minute size of the murine IVDs.
28744842	3	50	theme	destructive	677:687	arg1	preparation					689:699	the destructive preparation	673:699	the destructive preparation of the sample	673:713	Histology is the gold standard for examining the IVD structure, but it is susceptible to sectioning artifacts, spatial biases, and requires the destructive preparation of the sample.
28744842	6	51	theme	resonance	1276:1284	arg1	MRI					1295:1297	MRI	1295:1297	MRI	1295:1297	Moreover, we compared the imaging quality and quantitation of these in vitro models to magnetic resonance imaging (MRI) and histology.
28744842	6	51	theme	resonance	1276:1284	arg1	imaging					1286:1292	magnetic resonance imaging	1267:1292	magnetic resonance imaging (MRI)	1267:1298	Moreover, we compared the imaging quality and quantitation of these in vitro models to magnetic resonance imaging (MRI) and histology.
28744842	6	52	dep	in	1248:1249	arg1	vitro					1251:1255	vitro	1251:1255	vitro	1251:1255	Moreover, we compared the imaging quality and quantitation of these in vitro models to magnetic resonance imaging (MRI) and histology.
28744842	7	53	theme	discs	1458:1462	arg1	overtime					1464:1471	the discs overtime	1454:1471	the discs overtime	1454:1471	Stab puncture, trypsin injection, and collagenase injection all induced detectable and significant changes in structure and composition of the discs overtime.
28744842	2	54	used	used	307:310	arg2	models					290:295	Murine models	283:295	Murine models	283:295	Murine models are often used to investigate the mechanistic changes in the development, aging, and diseased states of the IVD, yet the detection of early degenerative changes in structure is challenging because of the minute size of the murine IVDs.
28744842	9	55	theme	structural	1676:1685	arg1	deteriorations					1687:1700	the structural deteriorations	1672:1700	the structural deteriorations	1672:1700	Contrast-enhanced microCT was also capable of monitoring the structural deteriorations via the changes in disc height and volume, and novel the nucleus pulposus intensity/disc intensity (NI/DI) parameter provides a surrogate measure of proteoglycan composition (R = 0.96).
28744842	3	56	theme	sample	708:713	arg1	preparation					689:699	the destructive preparation	673:699	the destructive preparation of the sample	673:713	Histology is the gold standard for examining the IVD structure, but it is susceptible to sectioning artifacts, spatial biases, and requires the destructive preparation of the sample.
28744842	3	57	theme	standard	555:562	arg1	Histology					533:541	Histology	533:541	Histology	533:541	Histology is the gold standard for examining the IVD structure, but it is susceptible to sectioning artifacts, spatial biases, and requires the destructive preparation of the sample.
28744842	3	57	theme	standard	555:562	arg1	gold					550:553	the gold	546:553	the gold standard for examining the IVD structure	546:594	Histology is the gold standard for examining the IVD structure, but it is susceptible to sectioning artifacts, spatial biases, and requires the destructive preparation of the sample.
28744842	1	58	theme	degenerative	222:233	arg1	changes					235:241	degenerative changes	222:241	degenerative changes that are associated with low back pain	222:280	The intervertebral disc (IVD) is susceptible to degenerative changes that are associated with low back pain.
28744842	9	59	theme	disc	1721:1724	arg1	height					1726:1731	disc height	1721:1731	disc height	1721:1731	Contrast-enhanced microCT was also capable of monitoring the structural deteriorations via the changes in disc height and volume, and novel the nucleus pulposus intensity/disc intensity (NI/DI) parameter provides a surrogate measure of proteoglycan composition (R = 0.96).
28744842	10	60	theme	composition	1973:1983	arg1	monitoring					1941:1950	the nondestructive monitoring	1922:1950	the nondestructive monitoring of the structure and composition of the IVD at very high resolutions	1922:2019	Overall, this approach allows for the nondestructive monitoring of the structure and composition of the IVD at very high resolutions.
28744842	3	61	theme	IVD	582:584	arg1	structure					586:594	the IVD structure	578:594	the IVD structure	578:594	Histology is the gold standard for examining the IVD structure, but it is susceptible to sectioning artifacts, spatial biases, and requires the destructive preparation of the sample.
28744842	2	62	theme	IVDs	527:530	arg1	size					508:511	the minute size	497:511	the minute size of the murine IVDs	497:530	Murine models are often used to investigate the mechanistic changes in the development, aging, and diseased states of the IVD, yet the detection of early degenerative changes in structure is challenging because of the minute size of the murine IVDs.
28744842	10	63	theme	high	2004:2007	arg1	resolutions					2009:2019	very high resolutions	1999:2019	very high resolutions	1999:2019	Overall, this approach allows for the nondestructive monitoring of the structure and composition of the IVD at very high resolutions.
28744842	5	64	theme	discs	1075:1079	arg1	models					1054:1059	in vitro nucleolytic- and mechanical injury- degeneration models	996:1059	in vitro nucleolytic- and mechanical injury- degeneration models of the murine discs	996:1079	In this work, we demonstrate utility of this approach to monitor the longitudinal changes of in vitro nucleolytic- and mechanical injury- degeneration models of the murine discs and introduce novel quantitative metrics to characterize the structure and composition of the IVD.
28744842	10	65	from	structure	1959:1967	arg1	resolutions					2009:2019	very high resolutions	1999:2019	very high resolutions	1999:2019	Overall, this approach allows for the nondestructive monitoring of the structure and composition of the IVD at very high resolutions.
28744842	4	66	theme	healthy	883:889	arg1	IVD					898:900	the intact healthy murine IVD	872:900	the intact healthy murine IVD	872:900	We have previously demonstrated the feasibility of using Ioversol for the contrast-enhanced micro-computed tomography (microCT) to visualize and quantitate the intact healthy murine IVD.
28744842	6	67	theme	imaging	1206:1212	arg1	quality					1214:1220	imaging quality	1206:1220	imaging quality	1206:1220	Moreover, we compared the imaging quality and quantitation of these in vitro models to magnetic resonance imaging (MRI) and histology.
28744842	5	68	theme	injury-	1033:1039	arg1	models					1054:1059	in vitro nucleolytic- and mechanical injury- degeneration models	996:1059	in vitro nucleolytic- and mechanical injury- degeneration models of the murine discs	996:1079	In this work, we demonstrate utility of this approach to monitor the longitudinal changes of in vitro nucleolytic- and mechanical injury- degeneration models of the murine discs and introduce novel quantitative metrics to characterize the structure and composition of the IVD.
28744842	9	69	theme	novel the	1749:1757	arg1	parameter					1809:1817	novel the nucleus pulposus intensity/disc intensity (NI/DI) parameter	1749:1817	novel the nucleus pulposus intensity/disc intensity (NI/DI) parameter	1749:1817	Contrast-enhanced microCT was also capable of monitoring the structural deteriorations via the changes in disc height and volume, and novel the nucleus pulposus intensity/disc intensity (NI/DI) parameter provides a surrogate measure of proteoglycan composition (R = 0.96).
28744842	0	70	theme	Discs	86:90	arg1	Analyses					46:53	The Quantitative Structural and Compositional Analyses	0:53	The Quantitative Structural and Compositional Analyses of Degenerating Intervertebral Discs	0:90	The Quantitative Structural and Compositional Analyses of Degenerating Intervertebral Discs Using Magnetic Resonance Imaging and Contrast-Enhanced Micro-Computed Tomography.
28744842	5	71	theme	models	1054:1059	arg1	changes					985:991	the longitudinal changes	968:991	the longitudinal changes of in vitro nucleolytic- and mechanical injury- degeneration models of the murine discs	968:1079	In this work, we demonstrate utility of this approach to monitor the longitudinal changes of in vitro nucleolytic- and mechanical injury- degeneration models of the murine discs and introduce novel quantitative metrics to characterize the structure and composition of the IVD.
28744842	1	72	theme	intervertebral	178:191	arg1	susceptible					207:217	susceptible	207:217	susceptible	207:217	The intervertebral disc (IVD) is susceptible to degenerative changes that are associated with low back pain.
28744842	1	72	theme	intervertebral	178:191	arg1	disc					193:196	The intervertebral disc	174:196	The intervertebral disc (IVD)	174:202	The intervertebral disc (IVD) is susceptible to degenerative changes that are associated with low back pain.
28744842	1	72	theme	intervertebral	178:191	arg1	IVD					199:201	IVD	199:201	IVD	199:201	The intervertebral disc (IVD) is susceptible to degenerative changes that are associated with low back pain.
28744842	9	73	theme	pulposus	1767:1774	arg1	parameter					1809:1817	novel the nucleus pulposus intensity/disc intensity (NI/DI) parameter	1749:1817	novel the nucleus pulposus intensity/disc intensity (NI/DI) parameter	1749:1817	Contrast-enhanced microCT was also capable of monitoring the structural deteriorations via the changes in disc height and volume, and novel the nucleus pulposus intensity/disc intensity (NI/DI) parameter provides a surrogate measure of proteoglycan composition (R = 0.96).
28744842	0	74	theme	Magnetic	98:105	arg1	Resonance					107:115	Magnetic Resonance	98:115	Magnetic Resonance Imaging	98:123	The Quantitative Structural and Compositional Analyses of Degenerating Intervertebral Discs Using Magnetic Resonance Imaging and Contrast-Enhanced Micro-Computed Tomography.
28744842	7	75	from	changes	1414:1420	arg1	composition					1439:1449	composition	1439:1449	composition	1439:1449	Stab puncture, trypsin injection, and collagenase injection all induced detectable and significant changes in structure and composition of the discs overtime.
28744842	7	75	from	changes	1414:1420	arg1	structure					1425:1433	structure	1425:1433	structure	1425:1433	Stab puncture, trypsin injection, and collagenase injection all induced detectable and significant changes in structure and composition of the discs overtime.
28744842	9	76	theme	intensity	1791:1799	arg1	parameter					1809:1817	novel the nucleus pulposus intensity/disc intensity (NI/DI) parameter	1749:1817	novel the nucleus pulposus intensity/disc intensity (NI/DI) parameter	1749:1817	Contrast-enhanced microCT was also capable of monitoring the structural deteriorations via the changes in disc height and volume, and novel the nucleus pulposus intensity/disc intensity (NI/DI) parameter provides a surrogate measure of proteoglycan composition (R = 0.96).
28744842	2	77	from	changes	450:456	arg1	structure					461:469	structure	461:469	structure	461:469	Murine models are often used to investigate the mechanistic changes in the development, aging, and diseased states of the IVD, yet the detection of early degenerative changes in structure is challenging because of the minute size of the murine IVDs.
28744842	9	78	theme	NI/DI	1802:1806	arg1	parameter					1809:1817	novel the nucleus pulposus intensity/disc intensity (NI/DI) parameter	1749:1817	novel the nucleus pulposus intensity/disc intensity (NI/DI) parameter	1749:1817	Contrast-enhanced microCT was also capable of monitoring the structural deteriorations via the changes in disc height and volume, and novel the nucleus pulposus intensity/disc intensity (NI/DI) parameter provides a surrogate measure of proteoglycan composition (R = 0.96).
28744842	2	79	from	structure	461:469	arg1	detection					418:426	the detection	414:426	the detection of early degenerative changes in structure	414:469	Murine models are often used to investigate the mechanistic changes in the development, aging, and diseased states of the IVD, yet the detection of early degenerative changes in structure is challenging because of the minute size of the murine IVDs.
28744842	0	80	theme	Contrast-Enhanced	129:145	arg1	Tomography					162:171	Contrast-Enhanced Micro-Computed Tomography	129:171	Contrast-Enhanced Micro-Computed Tomography	129:171	The Quantitative Structural and Compositional Analyses of Degenerating Intervertebral Discs Using Magnetic Resonance Imaging and Contrast-Enhanced Micro-Computed Tomography.
28744842	5	81	dep	structure	1142:1150	arg1	the					1138:1140	the	1138:1140	the	1138:1140	In this work, we demonstrate utility of this approach to monitor the longitudinal changes of in vitro nucleolytic- and mechanical injury- degeneration models of the murine discs and introduce novel quantitative metrics to characterize the structure and composition of the IVD.
28744842	8	82	theme	contrast-enhanced	1505:1521	arg1	microCT					1523:1529	contrast-enhanced microCT	1505:1529	contrast-enhanced microCT	1505:1529	Compared to MRI and histology, contrast-enhanced microCT produced superior images that capture the degenerative progression in these models.
28744842	0	83	theme	Quantitative	4:15	arg1	Analyses					46:53	The Quantitative Structural and Compositional Analyses	0:53	The Quantitative Structural and Compositional Analyses of Degenerating Intervertebral Discs	0:90	The Quantitative Structural and Compositional Analyses of Degenerating Intervertebral Discs Using Magnetic Resonance Imaging and Contrast-Enhanced Micro-Computed Tomography.
28744842	5	84	theme	quantitative	1101:1112	arg1	metrics					1114:1120	novel quantitative metrics	1095:1120	novel quantitative metrics	1095:1120	In this work, we demonstrate utility of this approach to monitor the longitudinal changes of in vitro nucleolytic- and mechanical injury- degeneration models of the murine discs and introduce novel quantitative metrics to characterize the structure and composition of the IVD.
28744842	10	85	from	resolutions	2009:2019	arg1	composition					1973:1983	composition	1973:1983	composition	1973:1983	Overall, this approach allows for the nondestructive monitoring of the structure and composition of the IVD at very high resolutions.
28744842	10	85	from	resolutions	2009:2019	arg1	IVD					1992:1994	the IVD	1988:1994	the IVD at very high resolutions	1988:2019	Overall, this approach allows for the nondestructive monitoring of the structure and composition of the IVD at very high resolutions.
28744842	10	85	from	resolutions	2009:2019	arg1	structure					1959:1967	structure	1959:1967	structure	1959:1967	Overall, this approach allows for the nondestructive monitoring of the structure and composition of the IVD at very high resolutions.
28744842	2	86	theme	degenerative	437:448	arg1	changes					450:456	early degenerative changes	431:456	early degenerative changes in structure	431:469	Murine models are often used to investigate the mechanistic changes in the development, aging, and diseased states of the IVD, yet the detection of early degenerative changes in structure is challenging because of the minute size of the murine IVDs.
28744842	5	87	theme	IVD	1175:1177	arg1	composition					1156:1166	composition	1156:1166	composition	1156:1166	In this work, we demonstrate utility of this approach to monitor the longitudinal changes of in vitro nucleolytic- and mechanical injury- degeneration models of the murine discs and introduce novel quantitative metrics to characterize the structure and composition of the IVD.
28744842	5	87	theme	IVD	1175:1177	arg1	structure					1142:1150	structure	1142:1150	structure	1142:1150	In this work, we demonstrate utility of this approach to monitor the longitudinal changes of in vitro nucleolytic- and mechanical injury- degeneration models of the murine discs and introduce novel quantitative metrics to characterize the structure and composition of the IVD.
28744842	3	88	theme	sectioning	622:631	arg1	artifacts					633:641	sectioning artifacts	622:641	sectioning artifacts	622:641	Histology is the gold standard for examining the IVD structure, but it is susceptible to sectioning artifacts, spatial biases, and requires the destructive preparation of the sample.
28744842	3	88	theme	sectioning	622:631	arg1	biases					652:657	spatial biases	644:657	spatial biases	644:657	Histology is the gold standard for examining the IVD structure, but it is susceptible to sectioning artifacts, spatial biases, and requires the destructive preparation of the sample.
28744842	2	89	dep	development	358:368	arg1	the					354:356	the	354:356	the	354:356	Murine models are often used to investigate the mechanistic changes in the development, aging, and diseased states of the IVD, yet the detection of early degenerative changes in structure is challenging because of the minute size of the murine IVDs.
28744842	5	90	theme	in	996:997	arg1	models					1054:1059	in vitro nucleolytic- and mechanical injury- degeneration models	996:1059	in vitro nucleolytic- and mechanical injury- degeneration models of the murine discs	996:1079	In this work, we demonstrate utility of this approach to monitor the longitudinal changes of in vitro nucleolytic- and mechanical injury- degeneration models of the murine discs and introduce novel quantitative metrics to characterize the structure and composition of the IVD.
28744842	9	91	theme	proteoglycan	1851:1862	arg1	R = 0.96					1877:1884	R = 0.96	1877:1884	R = 0.96	1877:1884	Contrast-enhanced microCT was also capable of monitoring the structural deteriorations via the changes in disc height and volume, and novel the nucleus pulposus intensity/disc intensity (NI/DI) parameter provides a surrogate measure of proteoglycan composition (R = 0.96).
28744842	9	91	theme	proteoglycan	1851:1862	arg1	composition					1864:1874	proteoglycan composition	1851:1874	proteoglycan composition (R = 0.96)	1851:1885	Contrast-enhanced microCT was also capable of monitoring the structural deteriorations via the changes in disc height and volume, and novel the nucleus pulposus intensity/disc intensity (NI/DI) parameter provides a surrogate measure of proteoglycan composition (R = 0.96).
28744842	8	92	theme	degenerative	1573:1584	arg1	progression					1586:1596	the degenerative progression	1569:1596	the degenerative progression in these models	1569:1612	Compared to MRI and histology, contrast-enhanced microCT produced superior images that capture the degenerative progression in these models.
28744842	4	93	theme	contrast-enhanced	790:806	arg1	microCT					835:841	microCT	835:841	microCT	835:841	We have previously demonstrated the feasibility of using Ioversol for the contrast-enhanced micro-computed tomography (microCT) to visualize and quantitate the intact healthy murine IVD.
28744842	4	93	theme	contrast-enhanced	790:806	arg1	tomography					823:832	the contrast-enhanced micro-computed tomography	786:832	the contrast-enhanced micro-computed tomography (microCT)	786:842	We have previously demonstrated the feasibility of using Ioversol for the contrast-enhanced micro-computed tomography (microCT) to visualize and quantitate the intact healthy murine IVD.
28744842	0	94	theme	Degenerating	58:69	arg1	Discs					86:90	Degenerating Intervertebral Discs	58:90	Degenerating Intervertebral Discs	58:90	The Quantitative Structural and Compositional Analyses of Degenerating Intervertebral Discs Using Magnetic Resonance Imaging and Contrast-Enhanced Micro-Computed Tomography.
28744842	5	95	theme	nucleolytic-	1005:1016	arg1	models					1054:1059	in vitro nucleolytic- and mechanical injury- degeneration models	996:1059	in vitro nucleolytic- and mechanical injury- degeneration models of the murine discs	996:1079	In this work, we demonstrate utility of this approach to monitor the longitudinal changes of in vitro nucleolytic- and mechanical injury- degeneration models of the murine discs and introduce novel quantitative metrics to characterize the structure and composition of the IVD.
26277353	2	0	with	ultrastructure	349:362	arg1	attention					402:410	special attention	394:410	special attention to tapetum cytology in Hovenia dulcis	394:448	The aim of this paper is to describe the ultrastructure of pollen grain ontogeny with special attention to tapetum cytology in Hovenia dulcis.
26277353	3	1	theme	development	482:492	arg1	stages					472:477	different stages	462:477	different stages of development	462:492	Anthers at different stages of development were processed for transmission and scanning electron microscopy, bright-field microscopy, and fluorescence microscopy.
26277353	6	2	contain	containing	795:804	arg2	carbohydrates					806:818	carbohydrates	806:818	carbohydrates	806:818	Large vesicles containing carbohydrates occur in the tapetal cell cytoplasm during the early stages of pollen development.
26277353	6	2	contain	containing	795:804	arg1	vesicles					786:793	Large vesicles	780:793	Large vesicles containing carbohydrates	780:818	Large vesicles containing carbohydrates occur in the tapetal cell cytoplasm during the early stages of pollen development.
26277353	5	3	theme	tapetal	733:739	arg1	cells					741:745	the tapetal cells	729:745	the tapetal cells	729:745	The ultrastructural changes observed during the development of the tapetal cells and pollen grains are described.
26277353	5	4	theme	ultrastructural	670:684	arg1	changes					686:692	The ultrastructural changes	666:692	The ultrastructural changes observed during the development of the tapetal cells and pollen grains	666:763	The ultrastructural changes observed during the development of the tapetal cells and pollen grains are described.
26277353	1	5	theme	microsporogenesis	236:252	arg1	aspects					221:227	general aspects	213:227	general aspects of the microsporogenesis, microgametogenesis, and anther development	213:296	Despite that there is some literature on pollen morphology of Rhamnaceae, studies addressing general aspects of the microsporogenesis, microgametogenesis, and anther development are rare.
26277353	5	6	theme	pollen	751:756	arg1	grains					758:763	pollen grains	751:763	pollen grains	751:763	The ultrastructural changes observed during the development of the tapetal cells and pollen grains are described.
26277353	0	7	from	Pollen	0:5	arg1	Rhamnaceae					58:67	Rhamnaceae	58:67	Rhamnaceae	58:67	Pollen and microsporangium development in Hovenia dulcis (Rhamnaceae): a different type of tapetal cell ultrastructure.
26277353	0	7	from	Pollen	0:5	arg1	dulcis					50:55	Hovenia dulcis	42:55	Hovenia dulcis (Rhamnaceae)	42:68	Pollen and microsporangium development in Hovenia dulcis (Rhamnaceae): a different type of tapetal cell ultrastructure.
26277353	8	8	theme	H.	1077:1078	arg1	ultrastructure					1004:1017	ultrastructure	1004:1017	ultrastructure	1004:1017	This is the first report on the ontogeny and ultrastructure of the pollen grain and related sporophytic structures of H. dulcis.
26277353	8	8	theme	H.	1077:1078	arg1	structures					1063:1072	related sporophytic structures	1043:1072	related sporophytic structures of H. dulcis	1043:1085	This is the first report on the ontogeny and ultrastructure of the pollen grain and related sporophytic structures of H. dulcis.
26277353	8	8	theme	H.	1077:1078	arg1	ontogeny					991:998	ontogeny	991:998	ontogeny	991:998	This is the first report on the ontogeny and ultrastructure of the pollen grain and related sporophytic structures of H. dulcis.
26277353	3	9	theme	scanning	530:537	arg1	microscopy					548:557	transmission and scanning electron microscopy	513:557	microscopy	548:557	Anthers at different stages of development were processed for transmission and scanning electron microscopy, bright-field microscopy, and fluorescence microscopy.
26277353	0	10	theme	different	73:81	arg1	type					83:86	a different type	71:86	Pollen and microsporangium development in Hovenia dulcis (Rhamnaceae): a different type of tapetal cell ultrastructure.	0:118	Pollen and microsporangium development in Hovenia dulcis (Rhamnaceae): a different type of tapetal cell ultrastructure.
26277353	6	11	theme	cell	841:844	arg1	cytoplasm					846:854	the tapetal cell cytoplasm	829:854	the tapetal cell cytoplasm	829:854	Large vesicles containing carbohydrates occur in the tapetal cell cytoplasm during the early stages of pollen development.
26277353	3	12	theme	electron	539:546	arg1	microscopy					548:557	transmission and scanning electron microscopy	513:557	microscopy	548:557	Anthers at different stages of development were processed for transmission and scanning electron microscopy, bright-field microscopy, and fluorescence microscopy.
26277353	1	13	theme	microgametogenesis	255:272	arg1	aspects					221:227	general aspects	213:227	general aspects of the microsporogenesis, microgametogenesis, and anther development	213:296	Despite that there is some literature on pollen morphology of Rhamnaceae, studies addressing general aspects of the microsporogenesis, microgametogenesis, and anther development are rare.
26277353	0	14	theme	tapetal	91:97	arg1	ultrastructure					104:117	tapetal cell ultrastructure	91:117	tapetal cell ultrastructure	91:117	Pollen and microsporangium development in Hovenia dulcis (Rhamnaceae): a different type of tapetal cell ultrastructure.
26277353	2	15	theme	special	394:400	arg1	attention					402:410	special attention	394:410	special attention to tapetum cytology in Hovenia dulcis	394:448	The aim of this paper is to describe the ultrastructure of pollen grain ontogeny with special attention to tapetum cytology in Hovenia dulcis.
26277353	3	16	theme	transmission	513:524	arg1	microscopy					548:557	transmission and scanning electron microscopy	513:557	microscopy	548:557	Anthers at different stages of development were processed for transmission and scanning electron microscopy, bright-field microscopy, and fluorescence microscopy.
26277353	2	17	theme	grain	374:378	arg1	ontogeny					380:387	pollen grain ontogeny	367:387	pollen grain ontogeny	367:387	The aim of this paper is to describe the ultrastructure of pollen grain ontogeny with special attention to tapetum cytology in Hovenia dulcis.
26277353	8	18	theme	grain	1033:1037	arg1	ultrastructure					1004:1017	ultrastructure	1004:1017	ultrastructure	1004:1017	This is the first report on the ontogeny and ultrastructure of the pollen grain and related sporophytic structures of H. dulcis.
26277353	8	18	theme	grain	1033:1037	arg1	structures					1063:1072	related sporophytic structures	1043:1072	related sporophytic structures of H. dulcis	1043:1085	This is the first report on the ontogeny and ultrastructure of the pollen grain and related sporophytic structures of H. dulcis.
26277353	8	18	theme	grain	1033:1037	arg1	ontogeny					991:998	ontogeny	991:998	ontogeny	991:998	This is the first report on the ontogeny and ultrastructure of the pollen grain and related sporophytic structures of H. dulcis.
26277353	2	19	theme	pollen	367:372	arg1	ontogeny					380:387	pollen grain ontogeny	367:387	pollen grain ontogeny	367:387	The aim of this paper is to describe the ultrastructure of pollen grain ontogeny with special attention to tapetum cytology in Hovenia dulcis.
26277353	8	20	theme	pollen	1026:1031	arg1	grain					1033:1037	the pollen grain	1022:1037	the pollen grain	1022:1037	This is the first report on the ontogeny and ultrastructure of the pollen grain and related sporophytic structures of H. dulcis.
26277353	3	21	theme	bright-field	560:571	arg1	microscopy					573:582	bright-field microscopy	560:582	bright-field microscopy	560:582	Anthers at different stages of development were processed for transmission and scanning electron microscopy, bright-field microscopy, and fluorescence microscopy.
26277353	1	22	theme	pollen	161:166	arg1	morphology					168:177	pollen morphology	161:177	pollen morphology of Rhamnaceae	161:191	Despite that there is some literature on pollen morphology of Rhamnaceae, studies addressing general aspects of the microsporogenesis, microgametogenesis, and anther development are rare.
26277353	3	23	from	stages	472:477	arg1	Anthers					451:457	Anthers	451:457	Anthers at different stages of development	451:492	Anthers at different stages of development were processed for transmission and scanning electron microscopy, bright-field microscopy, and fluorescence microscopy.
26277353	8	24	theme	sporophytic	1051:1061	arg1	structures					1063:1072	related sporophytic structures	1043:1072	related sporophytic structures of H. dulcis	1043:1085	This is the first report on the ontogeny and ultrastructure of the pollen grain and related sporophytic structures of H. dulcis.
26277353	2	25	theme	tapetum	415:421	arg1	cytology					423:430	tapetum cytology	415:430	tapetum cytology	415:430	The aim of this paper is to describe the ultrastructure of pollen grain ontogeny with special attention to tapetum cytology in Hovenia dulcis.
26277353	8	26	theme	related	1043:1049	arg1	structures					1063:1072	related sporophytic structures	1043:1072	related sporophytic structures of H. dulcis	1043:1085	This is the first report on the ontogeny and ultrastructure of the pollen grain and related sporophytic structures of H. dulcis.
26277353	0	27	theme	ultrastructure	104:117	arg1	type					83:86	a different type	71:86	Pollen and microsporangium development in Hovenia dulcis (Rhamnaceae): a different type of tapetal cell ultrastructure.	0:118	Pollen and microsporangium development in Hovenia dulcis (Rhamnaceae): a different type of tapetal cell ultrastructure.
26277353	6	28	theme	tapetal	833:839	arg1	cytoplasm					846:854	the tapetal cell cytoplasm	829:854	the tapetal cell cytoplasm	829:854	Large vesicles containing carbohydrates occur in the tapetal cell cytoplasm during the early stages of pollen development.
26277353	0	29	theme	microsporangium	11:25	arg1	development					27:37	microsporangium development	11:37	microsporangium development	11:37	Pollen and microsporangium development in Hovenia dulcis (Rhamnaceae): a different type of tapetal cell ultrastructure.
26277353	1	30	theme	anther	279:284	arg1	development					286:296	anther development	279:296	anther development	279:296	Despite that there is some literature on pollen morphology of Rhamnaceae, studies addressing general aspects of the microsporogenesis, microgametogenesis, and anther development are rare.
26277353	0	31	theme	cell	99:102	arg1	ultrastructure					104:117	tapetal cell ultrastructure	91:117	tapetal cell ultrastructure	91:117	Pollen and microsporangium development in Hovenia dulcis (Rhamnaceae): a different type of tapetal cell ultrastructure.
26277353	1	32	theme	Rhamnaceae	182:191	arg1	morphology					168:177	pollen morphology	161:177	pollen morphology of Rhamnaceae	161:191	Despite that there is some literature on pollen morphology of Rhamnaceae, studies addressing general aspects of the microsporogenesis, microgametogenesis, and anther development are rare.
26277353	0	33	theme	Hovenia	42:48	arg1	Rhamnaceae					58:67	Rhamnaceae	58:67	Rhamnaceae	58:67	Pollen and microsporangium development in Hovenia dulcis (Rhamnaceae): a different type of tapetal cell ultrastructure.
26277353	0	33	theme	Hovenia	42:48	arg1	dulcis					50:55	Hovenia dulcis	42:55	Hovenia dulcis (Rhamnaceae)	42:68	Pollen and microsporangium development in Hovenia dulcis (Rhamnaceae): a different type of tapetal cell ultrastructure.
26277353	1	34	theme	development	286:296	arg1	aspects					221:227	general aspects	213:227	general aspects of the microsporogenesis, microgametogenesis, and anther development	213:296	Despite that there is some literature on pollen morphology of Rhamnaceae, studies addressing general aspects of the microsporogenesis, microgametogenesis, and anther development are rare.
26277353	5	35	theme	cells	741:745	arg1	development					714:724	the development	710:724	the development of the tapetal cells and pollen grains	710:763	The ultrastructural changes observed during the development of the tapetal cells and pollen grains are described.
26277353	3	36	theme	fluorescence	589:600	arg1	microscopy					602:611	fluorescence microscopy	589:611	fluorescence microscopy	589:611	Anthers at different stages of development were processed for transmission and scanning electron microscopy, bright-field microscopy, and fluorescence microscopy.
26277353	5	37	theme	grains	758:763	arg1	development					714:724	the development	710:724	the development of the tapetal cells and pollen grains	710:763	The ultrastructural changes observed during the development of the tapetal cells and pollen grains are described.
26277353	2	38	theme	ontogeny	380:387	arg1	ultrastructure					349:362	the ultrastructure	345:362	the ultrastructure of pollen grain ontogeny with special attention to tapetum cytology in Hovenia dulcis	345:448	The aim of this paper is to describe the ultrastructure of pollen grain ontogeny with special attention to tapetum cytology in Hovenia dulcis.
26277353	8	39	dep	H.	1077:1078	arg1	dulcis					1080:1085	H. dulcis	1077:1085	H. dulcis	1077:1085	This is the first report on the ontogeny and ultrastructure of the pollen grain and related sporophytic structures of H. dulcis.
26277353	8	40	from	report	977:982	arg1	ultrastructure					1004:1017	ultrastructure	1004:1017	ultrastructure	1004:1017	This is the first report on the ontogeny and ultrastructure of the pollen grain and related sporophytic structures of H. dulcis.
26277353	8	40	from	report	977:982	arg1	structures					1063:1072	related sporophytic structures	1043:1072	related sporophytic structures of H. dulcis	1043:1085	This is the first report on the ontogeny and ultrastructure of the pollen grain and related sporophytic structures of H. dulcis.
26277353	8	40	from	report	977:982	arg1	ontogeny					991:998	ontogeny	991:998	ontogeny	991:998	This is the first report on the ontogeny and ultrastructure of the pollen grain and related sporophytic structures of H. dulcis.
26277353	6	41	theme	pollen	883:888	arg1	development					890:900	pollen development	883:900	pollen development	883:900	Large vesicles containing carbohydrates occur in the tapetal cell cytoplasm during the early stages of pollen development.
26277353	2	42	theme	paper	324:328	arg1	aim					312:314	The aim	308:314	The aim of this paper	308:328	The aim of this paper is to describe the ultrastructure of pollen grain ontogeny with special attention to tapetum cytology in Hovenia dulcis.
26277353	2	43	theme	Hovenia	435:441	arg1	dulcis					443:448	Hovenia dulcis	435:448	Hovenia dulcis	435:448	The aim of this paper is to describe the ultrastructure of pollen grain ontogeny with special attention to tapetum cytology in Hovenia dulcis.
26277353	6	44	theme	Large	780:784	arg1	vesicles					786:793	Large vesicles	780:793	Large vesicles containing carbohydrates	780:818	Large vesicles containing carbohydrates occur in the tapetal cell cytoplasm during the early stages of pollen development.
26277353	6	45	theme	development	890:900	arg1	stages					873:878	the early stages	863:878	the early stages of pollen development	863:900	Large vesicles containing carbohydrates occur in the tapetal cell cytoplasm during the early stages of pollen development.
26277353	8	46	dep	ontogeny	991:998	arg1	the					987:989	the	987:989	the	987:989	This is the first report on the ontogeny and ultrastructure of the pollen grain and related sporophytic structures of H. dulcis.
26277353	2	47	from	attention	402:410	arg1	dulcis					443:448	Hovenia dulcis	435:448	Hovenia dulcis	435:448	The aim of this paper is to describe the ultrastructure of pollen grain ontogeny with special attention to tapetum cytology in Hovenia dulcis.
26277353	4	48	theme	Different	614:622	arg1	reactions					638:646	Different histochemical reactions	614:646	Different histochemical reactions	614:646	Different histochemical reactions were carried out.
26277353	6	49	theme	early	867:871	arg1	stages					873:878	the early stages	863:878	the early stages of pollen development	863:900	Large vesicles containing carbohydrates occur in the tapetal cell cytoplasm during the early stages of pollen development.
26277353	3	50	theme	different	462:470	arg1	stages					472:477	different stages	462:477	different stages of development	462:492	Anthers at different stages of development were processed for transmission and scanning electron microscopy, bright-field microscopy, and fluorescence microscopy.
26277353	1	51	theme	general	213:219	arg1	aspects					221:227	general aspects	213:227	general aspects of the microsporogenesis, microgametogenesis, and anther development	213:296	Despite that there is some literature on pollen morphology of Rhamnaceae, studies addressing general aspects of the microsporogenesis, microgametogenesis, and anther development are rare.
26277353	1	52	dep	microsporogenesis	236:252	arg1	the					232:234	the	232:234	the	232:234	Despite that there is some literature on pollen morphology of Rhamnaceae, studies addressing general aspects of the microsporogenesis, microgametogenesis, and anther development are rare.
26277353	0	53	from	development	27:37	arg1	Rhamnaceae					58:67	Rhamnaceae	58:67	Rhamnaceae	58:67	Pollen and microsporangium development in Hovenia dulcis (Rhamnaceae): a different type of tapetal cell ultrastructure.
26277353	0	53	from	development	27:37	arg1	dulcis					50:55	Hovenia dulcis	42:55	Hovenia dulcis (Rhamnaceae)	42:68	Pollen and microsporangium development in Hovenia dulcis (Rhamnaceae): a different type of tapetal cell ultrastructure.
26277353	0	54	dep	Pollen	0:5	arg1	type					83:86	a different type	71:86	Pollen and microsporangium development in Hovenia dulcis (Rhamnaceae): a different type of tapetal cell ultrastructure.	0:118	Pollen and microsporangium development in Hovenia dulcis (Rhamnaceae): a different type of tapetal cell ultrastructure.
26277353	1	55	from	literature	147:156	arg1	morphology					168:177	pollen morphology	161:177	pollen morphology of Rhamnaceae	161:191	Despite that there is some literature on pollen morphology of Rhamnaceae, studies addressing general aspects of the microsporogenesis, microgametogenesis, and anther development are rare.
26277353	8	56	theme	first	971:975	arg1	This					959:962	This	959:962	This	959:962	This is the first report on the ontogeny and ultrastructure of the pollen grain and related sporophytic structures of H. dulcis.
26277353	8	56	theme	first	971:975	arg1	report					977:982	the first report	967:982	the first report on the ontogeny and ultrastructure of the pollen grain and related sporophytic structures of H. dulcis	967:1085	This is the first report on the ontogeny and ultrastructure of the pollen grain and related sporophytic structures of H. dulcis.
26277353	4	57	theme	histochemical	624:636	arg1	reactions					638:646	Different histochemical reactions	614:646	Different histochemical reactions	614:646	Different histochemical reactions were carried out.
26460376	4	0	theme	individual	878:887	arg1	Meanwhile					849:857	Meanwhile	849:857	Meanwhile	849:857	Meanwhile, the corresponding individual selector CSPs were also prepared, respectively, for the sake of comparison with biselector CSPs.
26460376	4	0	theme	individual	878:887	arg1	CSPs					898:901	the corresponding individual selector CSPs	860:901	the corresponding individual selector CSPs	860:901	Meanwhile, the corresponding individual selector CSPs were also prepared, respectively, for the sake of comparison with biselector CSPs.
26460376	1	1	theme	powerful	234:241	arg1	performance					261:271	their powerful enantioseparation performance	228:271	their powerful enantioseparation performance	228:271	Amylose derivatives chiral stationary phases (CSPs), some of which are commercially available, are well known for their powerful enantioseparation performance.
26460376	4	2	theme	corresponding	864:876	arg1	Meanwhile					849:857	Meanwhile	849:857	Meanwhile	849:857	Meanwhile, the corresponding individual selector CSPs were also prepared, respectively, for the sake of comparison with biselector CSPs.
26460376	4	2	theme	corresponding	864:876	arg1	CSPs					898:901	the corresponding individual selector CSPs	860:901	the corresponding individual selector CSPs	860:901	Meanwhile, the corresponding individual selector CSPs were also prepared, respectively, for the sake of comparison with biselector CSPs.
26460376	6	3	theme	elute	1296:1300	arg1	orders					1302:1307	the elute orders	1292:1307	the elute orders of enantiomers	1292:1322	In addition, the influence of composition and interaction of amylose tris(3,5-dimethylphenylcarbamate) and chitin bis(3-chloro-4-methylphenylcarbamate) in the biselector CSPs on the chiral recognition and the elute orders of enantiomers was also discussed in detail.
26460376	1	4	theme	enantioseparation	243:259	arg1	performance					261:271	their powerful enantioseparation performance	228:271	their powerful enantioseparation performance	228:271	Amylose derivatives chiral stationary phases (CSPs), some of which are commercially available, are well known for their powerful enantioseparation performance.
26460376	5	5	theme	recognition	997:1007	arg1	capacity					1009:1016	The chiral recognition capacity	986:1016	The chiral recognition capacity	986:1016	The chiral recognition capacity and solvent tolerance of five CSPs were systematically investigated.
26460376	6	6	theme	tris	1156:1159	arg1	interaction					1133:1143	interaction	1133:1143	interaction	1133:1143	In addition, the influence of composition and interaction of amylose tris(3,5-dimethylphenylcarbamate) and chitin bis(3-chloro-4-methylphenylcarbamate) in the biselector CSPs on the chiral recognition and the elute orders of enantiomers was also discussed in detail.
26460376	6	6	theme	tris	1156:1159	arg1	composition					1117:1127	composition	1117:1127	composition	1117:1127	In addition, the influence of composition and interaction of amylose tris(3,5-dimethylphenylcarbamate) and chitin bis(3-chloro-4-methylphenylcarbamate) in the biselector CSPs on the chiral recognition and the elute orders of enantiomers was also discussed in detail.
26460376	0	7	theme	Chitin	94:99	arg1	Derivatives					101:111	Chitin Derivatives	94:111	Chitin Derivatives	94:111	Preparation and Enantioseparation of Biselector Chiral Stationary Phases Based on Amylose and Chitin Derivatives.
26460376	4	8	theme	selector	889:896	arg1	Meanwhile					849:857	Meanwhile	849:857	Meanwhile	849:857	Meanwhile, the corresponding individual selector CSPs were also prepared, respectively, for the sake of comparison with biselector CSPs.
26460376	4	8	theme	selector	889:896	arg1	CSPs					898:901	the corresponding individual selector CSPs	860:901	the corresponding individual selector CSPs	860:901	Meanwhile, the corresponding individual selector CSPs were also prepared, respectively, for the sake of comparison with biselector CSPs.
26460376	3	9	theme	amylose	533:539	arg1	capability					519:528	the chiral recognition capability	496:528	the chiral recognition capability of amylose tris(3,5-dimethylphenylcarbamate)	496:573	In order to overcome the defect as well as maintain the chiral recognition capability of amylose tris(3,5-dimethylphenylcarbamate), chitin bis(3-chloro-4-methylphenylcarbamate) with good stability and good chiral recognition capability was blended with the amylose derivative at different ratios, and the resulting blends were further coated onto 3-aminopropyl silica gel to obtain three biselector CSPs.
26460376	3	10	with	bis	583:585	arg1	capability					669:678	good chiral recognition capability	645:678	good chiral recognition capability	645:678	In order to overcome the defect as well as maintain the chiral recognition capability of amylose tris(3,5-dimethylphenylcarbamate), chitin bis(3-chloro-4-methylphenylcarbamate) with good stability and good chiral recognition capability was blended with the amylose derivative at different ratios, and the resulting blends were further coated onto 3-aminopropyl silica gel to obtain three biselector CSPs.
26460376	3	10	with	bis	583:585	arg1	stability					631:639	good stability	626:639	good stability	626:639	In order to overcome the defect as well as maintain the chiral recognition capability of amylose tris(3,5-dimethylphenylcarbamate), chitin bis(3-chloro-4-methylphenylcarbamate) with good stability and good chiral recognition capability was blended with the amylose derivative at different ratios, and the resulting blends were further coated onto 3-aminopropyl silica gel to obtain three biselector CSPs.
26460376	6	11	theme	amylose	1148:1154	arg1	3,5-dimethylphenylcarbamate					1161:1187	3,5-dimethylphenylcarbamate	1161:1187	3,5-dimethylphenylcarbamate	1161:1187	In addition, the influence of composition and interaction of amylose tris(3,5-dimethylphenylcarbamate) and chitin bis(3-chloro-4-methylphenylcarbamate) in the biselector CSPs on the chiral recognition and the elute orders of enantiomers was also discussed in detail.
26460376	6	11	theme	amylose	1148:1154	arg1	tris					1156:1159	amylose tris	1148:1159	amylose tris(3,5-dimethylphenylcarbamate)	1148:1188	In addition, the influence of composition and interaction of amylose tris(3,5-dimethylphenylcarbamate) and chitin bis(3-chloro-4-methylphenylcarbamate) in the biselector CSPs on the chiral recognition and the elute orders of enantiomers was also discussed in detail.
26460376	1	12	dep	derivatives	122:132	arg1	CSPs					160:163	CSPs	160:163	CSPs	160:163	Amylose derivatives chiral stationary phases (CSPs), some of which are commercially available, are well known for their powerful enantioseparation performance.
26460376	1	12	dep	derivatives	122:132	arg1	phases					152:157	chiral stationary phases	134:157	chiral stationary phases (CSPs)	134:164	Amylose derivatives chiral stationary phases (CSPs), some of which are commercially available, are well known for their powerful enantioseparation performance.
26460376	6	13	theme	enantiomers	1312:1322	arg1	orders					1302:1307	the elute orders	1292:1307	the elute orders of enantiomers	1292:1322	In addition, the influence of composition and interaction of amylose tris(3,5-dimethylphenylcarbamate) and chitin bis(3-chloro-4-methylphenylcarbamate) in the biselector CSPs on the chiral recognition and the elute orders of enantiomers was also discussed in detail.
26460376	6	13	theme	enantiomers	1312:1322	arg1	recognition					1276:1286	the chiral recognition	1265:1286	the chiral recognition	1265:1286	In addition, the influence of composition and interaction of amylose tris(3,5-dimethylphenylcarbamate) and chitin bis(3-chloro-4-methylphenylcarbamate) in the biselector CSPs on the chiral recognition and the elute orders of enantiomers was also discussed in detail.
26460376	4	14	theme	comparison	953:962	arg1	sake					945:948	the sake	941:948	the sake of comparison with biselector CSPs	941:983	Meanwhile, the corresponding individual selector CSPs were also prepared, respectively, for the sake of comparison with biselector CSPs.
26460376	3	15	theme	biselector	832:841	arg1	CSPs					843:846	three biselector CSPs	826:846	three biselector CSPs	826:846	In order to overcome the defect as well as maintain the chiral recognition capability of amylose tris(3,5-dimethylphenylcarbamate), chitin bis(3-chloro-4-methylphenylcarbamate) with good stability and good chiral recognition capability was blended with the amylose derivative at different ratios, and the resulting blends were further coated onto 3-aminopropyl silica gel to obtain three biselector CSPs.
26460376	3	16	theme	different	723:731	arg1	ratios					733:738	different ratios	723:738	different ratios	723:738	In order to overcome the defect as well as maintain the chiral recognition capability of amylose tris(3,5-dimethylphenylcarbamate), chitin bis(3-chloro-4-methylphenylcarbamate) with good stability and good chiral recognition capability was blended with the amylose derivative at different ratios, and the resulting blends were further coated onto 3-aminopropyl silica gel to obtain three biselector CSPs.
26460376	2	17	theme	swelling	309:316	arg1	properties					318:327	the dissolution or swelling properties	290:327	the dissolution or swelling properties of amylose derivatives	290:350	However, due to the dissolution or swelling properties of amylose derivatives, this type of CSPs prepared by coating method exhibit poor solvent tolerance and stability.
26460376	3	18	theme	resulting	749:757	arg1	blends					759:764	the resulting blends	745:764	the resulting blends	745:764	In order to overcome the defect as well as maintain the chiral recognition capability of amylose tris(3,5-dimethylphenylcarbamate), chitin bis(3-chloro-4-methylphenylcarbamate) with good stability and good chiral recognition capability was blended with the amylose derivative at different ratios, and the resulting blends were further coated onto 3-aminopropyl silica gel to obtain three biselector CSPs.
26460376	6	19	theme	chiral	1269:1274	arg1	recognition					1276:1286	the chiral recognition	1265:1286	the chiral recognition	1265:1286	In addition, the influence of composition and interaction of amylose tris(3,5-dimethylphenylcarbamate) and chitin bis(3-chloro-4-methylphenylcarbamate) in the biselector CSPs on the chiral recognition and the elute orders of enantiomers was also discussed in detail.
26460376	2	20	theme	CSPs	366:369	arg1	type					358:361	this type	353:361	this type of CSPs prepared by coating method	353:396	However, due to the dissolution or swelling properties of amylose derivatives, this type of CSPs prepared by coating method exhibit poor solvent tolerance and stability.
26460376	6	21	theme	biselector	1246:1255	arg1	CSPs					1257:1260	the biselector CSPs	1242:1260	the biselector CSPs	1242:1260	In addition, the influence of composition and interaction of amylose tris(3,5-dimethylphenylcarbamate) and chitin bis(3-chloro-4-methylphenylcarbamate) in the biselector CSPs on the chiral recognition and the elute orders of enantiomers was also discussed in detail.
26460376	2	22	theme	dissolution	294:304	arg1	properties					318:327	the dissolution or swelling properties	290:327	the dissolution or swelling properties of amylose derivatives	290:350	However, due to the dissolution or swelling properties of amylose derivatives, this type of CSPs prepared by coating method exhibit poor solvent tolerance and stability.
26460376	2	23	theme	solvent	411:417	arg1	tolerance					419:427	poor solvent tolerance	406:427	poor solvent tolerance	406:427	However, due to the dissolution or swelling properties of amylose derivatives, this type of CSPs prepared by coating method exhibit poor solvent tolerance and stability.
26460376	3	24	theme	recognition	507:517	arg1	capability					519:528	the chiral recognition capability	496:528	the chiral recognition capability of amylose tris(3,5-dimethylphenylcarbamate)	496:573	In order to overcome the defect as well as maintain the chiral recognition capability of amylose tris(3,5-dimethylphenylcarbamate), chitin bis(3-chloro-4-methylphenylcarbamate) with good stability and good chiral recognition capability was blended with the amylose derivative at different ratios, and the resulting blends were further coated onto 3-aminopropyl silica gel to obtain three biselector CSPs.
26460376	3	25	theme	good	645:648	arg1	capability					669:678	good chiral recognition capability	645:678	good chiral recognition capability	645:678	In order to overcome the defect as well as maintain the chiral recognition capability of amylose tris(3,5-dimethylphenylcarbamate), chitin bis(3-chloro-4-methylphenylcarbamate) with good stability and good chiral recognition capability was blended with the amylose derivative at different ratios, and the resulting blends were further coated onto 3-aminopropyl silica gel to obtain three biselector CSPs.
26460376	1	26	theme	Amylose	114:120	arg1	derivatives					122:132	Amylose derivatives	114:132	Amylose derivatives	114:132	Amylose derivatives chiral stationary phases (CSPs), some of which are commercially available, are well known for their powerful enantioseparation performance.
26460376	2	27	theme	poor	406:409	arg1	tolerance					419:427	poor solvent tolerance	406:427	poor solvent tolerance	406:427	However, due to the dissolution or swelling properties of amylose derivatives, this type of CSPs prepared by coating method exhibit poor solvent tolerance and stability.
26460376	0	28	theme	Chiral	48:53	arg1	Phases					66:71	Biselector Chiral Stationary Phases	37:71	Biselector Chiral Stationary Phases	37:71	Preparation and Enantioseparation of Biselector Chiral Stationary Phases Based on Amylose and Chitin Derivatives.
26460376	2	29	theme	amylose	332:338	arg1	derivatives					340:350	amylose derivatives	332:350	amylose derivatives	332:350	However, due to the dissolution or swelling properties of amylose derivatives, this type of CSPs prepared by coating method exhibit poor solvent tolerance and stability.
26460376	3	30	theme	chiral	650:655	arg1	capability					669:678	good chiral recognition capability	645:678	good chiral recognition capability	645:678	In order to overcome the defect as well as maintain the chiral recognition capability of amylose tris(3,5-dimethylphenylcarbamate), chitin bis(3-chloro-4-methylphenylcarbamate) with good stability and good chiral recognition capability was blended with the amylose derivative at different ratios, and the resulting blends were further coated onto 3-aminopropyl silica gel to obtain three biselector CSPs.
26460376	5	31	theme	solvent	1022:1028	arg1	tolerance					1030:1038	solvent tolerance	1022:1038	solvent tolerance	1022:1038	The chiral recognition capacity and solvent tolerance of five CSPs were systematically investigated.
26460376	0	32	theme	Biselector	37:46	arg1	Phases					66:71	Biselector Chiral Stationary Phases	37:71	Biselector Chiral Stationary Phases	37:71	Preparation and Enantioseparation of Biselector Chiral Stationary Phases Based on Amylose and Chitin Derivatives.
26460376	6	33	from	interaction	1133:1143	arg1	CSPs					1257:1260	the biselector CSPs	1242:1260	the biselector CSPs	1242:1260	In addition, the influence of composition and interaction of amylose tris(3,5-dimethylphenylcarbamate) and chitin bis(3-chloro-4-methylphenylcarbamate) in the biselector CSPs on the chiral recognition and the elute orders of enantiomers was also discussed in detail.
26460376	0	34	theme	Phases	66:71	arg1	Enantioseparation					16:32	Enantioseparation	16:32	Enantioseparation	16:32	Preparation and Enantioseparation of Biselector Chiral Stationary Phases Based on Amylose and Chitin Derivatives.
26460376	0	34	theme	Phases	66:71	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and Enantioseparation of Biselector Chiral Stationary Phases Based on Amylose and Chitin Derivatives.
26460376	2	35	theme	derivatives	340:350	arg1	properties					318:327	the dissolution or swelling properties	290:327	the dissolution or swelling properties of amylose derivatives	290:350	However, due to the dissolution or swelling properties of amylose derivatives, this type of CSPs prepared by coating method exhibit poor solvent tolerance and stability.
26460376	0	36	theme	Stationary	55:64	arg1	Phases					66:71	Biselector Chiral Stationary Phases	37:71	Biselector Chiral Stationary Phases	37:71	Preparation and Enantioseparation of Biselector Chiral Stationary Phases Based on Amylose and Chitin Derivatives.
26460376	6	37	theme	bis	1201:1203	arg1	interaction					1133:1143	interaction	1133:1143	interaction	1133:1143	In addition, the influence of composition and interaction of amylose tris(3,5-dimethylphenylcarbamate) and chitin bis(3-chloro-4-methylphenylcarbamate) in the biselector CSPs on the chiral recognition and the elute orders of enantiomers was also discussed in detail.
26460376	6	37	theme	bis	1201:1203	arg1	composition					1117:1127	composition	1117:1127	composition	1117:1127	In addition, the influence of composition and interaction of amylose tris(3,5-dimethylphenylcarbamate) and chitin bis(3-chloro-4-methylphenylcarbamate) in the biselector CSPs on the chiral recognition and the elute orders of enantiomers was also discussed in detail.
26460376	5	38	theme	CSPs	1048:1051	arg1	capacity					1009:1016	The chiral recognition capacity	986:1016	The chiral recognition capacity	986:1016	The chiral recognition capacity and solvent tolerance of five CSPs were systematically investigated.
26460376	5	38	theme	CSPs	1048:1051	arg1	tolerance					1030:1038	solvent tolerance	1022:1038	solvent tolerance	1022:1038	The chiral recognition capacity and solvent tolerance of five CSPs were systematically investigated.
26460376	3	39	theme	chitin	576:581	arg1	bis					583:585	chitin bis	576:585	chitin bis(3-chloro-4-methylphenylcarbamate) with good stability and good chiral recognition capability	576:678	In order to overcome the defect as well as maintain the chiral recognition capability of amylose tris(3,5-dimethylphenylcarbamate), chitin bis(3-chloro-4-methylphenylcarbamate) with good stability and good chiral recognition capability was blended with the amylose derivative at different ratios, and the resulting blends were further coated onto 3-aminopropyl silica gel to obtain three biselector CSPs.
26460376	3	39	theme	chitin	576:581	arg1	3-chloro-4-methylphenylcarbamate					587:618	3-chloro-4-methylphenylcarbamate	587:618	3-chloro-4-methylphenylcarbamate	587:618	In order to overcome the defect as well as maintain the chiral recognition capability of amylose tris(3,5-dimethylphenylcarbamate), chitin bis(3-chloro-4-methylphenylcarbamate) with good stability and good chiral recognition capability was blended with the amylose derivative at different ratios, and the resulting blends were further coated onto 3-aminopropyl silica gel to obtain three biselector CSPs.
26460376	1	40	theme	chiral	134:139	arg1	CSPs					160:163	CSPs	160:163	CSPs	160:163	Amylose derivatives chiral stationary phases (CSPs), some of which are commercially available, are well known for their powerful enantioseparation performance.
26460376	1	40	theme	chiral	134:139	arg1	phases					152:157	chiral stationary phases	134:157	chiral stationary phases (CSPs)	134:164	Amylose derivatives chiral stationary phases (CSPs), some of which are commercially available, are well known for their powerful enantioseparation performance.
26460376	6	41	from	CSPs	1257:1260	arg1	influence					1104:1112	the influence	1100:1112	the influence of composition and interaction of amylose tris(3,5-dimethylphenylcarbamate) and chitin bis(3-chloro-4-methylphenylcarbamate) in the biselector CSPs on the chiral recognition and the elute orders of enantiomers	1100:1322	In addition, the influence of composition and interaction of amylose tris(3,5-dimethylphenylcarbamate) and chitin bis(3-chloro-4-methylphenylcarbamate) in the biselector CSPs on the chiral recognition and the elute orders of enantiomers was also discussed in detail.
26460376	5	42	theme	chiral	990:995	arg1	capacity					1009:1016	The chiral recognition capacity	986:1016	The chiral recognition capacity	986:1016	The chiral recognition capacity and solvent tolerance of five CSPs were systematically investigated.
26460376	1	43	theme	stationary	141:150	arg1	CSPs					160:163	CSPs	160:163	CSPs	160:163	Amylose derivatives chiral stationary phases (CSPs), some of which are commercially available, are well known for their powerful enantioseparation performance.
26460376	1	43	theme	stationary	141:150	arg1	phases					152:157	chiral stationary phases	134:157	chiral stationary phases (CSPs)	134:164	Amylose derivatives chiral stationary phases (CSPs), some of which are commercially available, are well known for their powerful enantioseparation performance.
26460376	3	44	theme	recognition	657:667	arg1	capability					669:678	good chiral recognition capability	645:678	good chiral recognition capability	645:678	In order to overcome the defect as well as maintain the chiral recognition capability of amylose tris(3,5-dimethylphenylcarbamate), chitin bis(3-chloro-4-methylphenylcarbamate) with good stability and good chiral recognition capability was blended with the amylose derivative at different ratios, and the resulting blends were further coated onto 3-aminopropyl silica gel to obtain three biselector CSPs.
26460376	3	45	theme	good	626:629	arg1	stability					631:639	good stability	626:639	good stability	626:639	In order to overcome the defect as well as maintain the chiral recognition capability of amylose tris(3,5-dimethylphenylcarbamate), chitin bis(3-chloro-4-methylphenylcarbamate) with good stability and good chiral recognition capability was blended with the amylose derivative at different ratios, and the resulting blends were further coated onto 3-aminopropyl silica gel to obtain three biselector CSPs.
26460376	6	46	from	influence	1104:1112	arg1	CSPs					1257:1260	the biselector CSPs	1242:1260	the biselector CSPs	1242:1260	In addition, the influence of composition and interaction of amylose tris(3,5-dimethylphenylcarbamate) and chitin bis(3-chloro-4-methylphenylcarbamate) in the biselector CSPs on the chiral recognition and the elute orders of enantiomers was also discussed in detail.
26460376	6	46	from	influence	1104:1112	arg1	recognition					1276:1286	the chiral recognition	1265:1286	the chiral recognition	1265:1286	In addition, the influence of composition and interaction of amylose tris(3,5-dimethylphenylcarbamate) and chitin bis(3-chloro-4-methylphenylcarbamate) in the biselector CSPs on the chiral recognition and the elute orders of enantiomers was also discussed in detail.
26460376	6	46	from	influence	1104:1112	arg1	orders					1302:1307	the elute orders	1292:1307	the elute orders of enantiomers	1292:1322	In addition, the influence of composition and interaction of amylose tris(3,5-dimethylphenylcarbamate) and chitin bis(3-chloro-4-methylphenylcarbamate) in the biselector CSPs on the chiral recognition and the elute orders of enantiomers was also discussed in detail.
26460376	3	47	dep	amylose	533:539	arg1	3,5-dimethylphenylcarbamate					546:572	3,5-dimethylphenylcarbamate	546:572	3,5-dimethylphenylcarbamate	546:572	In order to overcome the defect as well as maintain the chiral recognition capability of amylose tris(3,5-dimethylphenylcarbamate), chitin bis(3-chloro-4-methylphenylcarbamate) with good stability and good chiral recognition capability was blended with the amylose derivative at different ratios, and the resulting blends were further coated onto 3-aminopropyl silica gel to obtain three biselector CSPs.
26460376	3	47	dep	amylose	533:539	arg1	tris					541:544	tris	541:544	amylose tris(3,5-dimethylphenylcarbamate)	533:573	In order to overcome the defect as well as maintain the chiral recognition capability of amylose tris(3,5-dimethylphenylcarbamate), chitin bis(3-chloro-4-methylphenylcarbamate) with good stability and good chiral recognition capability was blended with the amylose derivative at different ratios, and the resulting blends were further coated onto 3-aminopropyl silica gel to obtain three biselector CSPs.
26460376	6	48	theme	interaction	1133:1143	arg1	influence					1104:1112	the influence	1100:1112	the influence of composition and interaction of amylose tris(3,5-dimethylphenylcarbamate) and chitin bis(3-chloro-4-methylphenylcarbamate) in the biselector CSPs on the chiral recognition and the elute orders of enantiomers	1100:1322	In addition, the influence of composition and interaction of amylose tris(3,5-dimethylphenylcarbamate) and chitin bis(3-chloro-4-methylphenylcarbamate) in the biselector CSPs on the chiral recognition and the elute orders of enantiomers was also discussed in detail.
26460376	4	49	with	comparison	953:962	arg1	CSPs					980:983	biselector CSPs	969:983	biselector CSPs	969:983	Meanwhile, the corresponding individual selector CSPs were also prepared, respectively, for the sake of comparison with biselector CSPs.
26460376	6	50	theme	composition	1117:1127	arg1	influence					1104:1112	the influence	1100:1112	the influence of composition and interaction of amylose tris(3,5-dimethylphenylcarbamate) and chitin bis(3-chloro-4-methylphenylcarbamate) in the biselector CSPs on the chiral recognition and the elute orders of enantiomers	1100:1322	In addition, the influence of composition and interaction of amylose tris(3,5-dimethylphenylcarbamate) and chitin bis(3-chloro-4-methylphenylcarbamate) in the biselector CSPs on the chiral recognition and the elute orders of enantiomers was also discussed in detail.
26460376	6	51	theme	chitin	1194:1199	arg1	3-chloro-4-methylphenylcarbamate					1205:1236	3-chloro-4-methylphenylcarbamate	1205:1236	3-chloro-4-methylphenylcarbamate	1205:1236	In addition, the influence of composition and interaction of amylose tris(3,5-dimethylphenylcarbamate) and chitin bis(3-chloro-4-methylphenylcarbamate) in the biselector CSPs on the chiral recognition and the elute orders of enantiomers was also discussed in detail.
26460376	6	51	theme	chitin	1194:1199	arg1	bis					1201:1203	chitin bis	1194:1203	chitin bis(3-chloro-4-methylphenylcarbamate)	1194:1237	In addition, the influence of composition and interaction of amylose tris(3,5-dimethylphenylcarbamate) and chitin bis(3-chloro-4-methylphenylcarbamate) in the biselector CSPs on the chiral recognition and the elute orders of enantiomers was also discussed in detail.
26460376	3	52	theme	3-aminopropyl	791:803	arg1	gel					812:814	3-aminopropyl silica gel	791:814	3-aminopropyl silica gel	791:814	In order to overcome the defect as well as maintain the chiral recognition capability of amylose tris(3,5-dimethylphenylcarbamate), chitin bis(3-chloro-4-methylphenylcarbamate) with good stability and good chiral recognition capability was blended with the amylose derivative at different ratios, and the resulting blends were further coated onto 3-aminopropyl silica gel to obtain three biselector CSPs.
26460376	4	53	theme	biselector	969:978	arg1	CSPs					980:983	biselector CSPs	969:983	biselector CSPs	969:983	Meanwhile, the corresponding individual selector CSPs were also prepared, respectively, for the sake of comparison with biselector CSPs.
26460376	3	54	theme	amylose	701:707	arg1	derivative					709:718	the amylose derivative	697:718	the amylose derivative	697:718	In order to overcome the defect as well as maintain the chiral recognition capability of amylose tris(3,5-dimethylphenylcarbamate), chitin bis(3-chloro-4-methylphenylcarbamate) with good stability and good chiral recognition capability was blended with the amylose derivative at different ratios, and the resulting blends were further coated onto 3-aminopropyl silica gel to obtain three biselector CSPs.
26460376	3	55	dep	overcome	456:463	arg1	maintain					487:494	maintain	487:494	maintain the chiral recognition capability of amylose tris(3,5-dimethylphenylcarbamate)	487:573	In order to overcome the defect as well as maintain the chiral recognition capability of amylose tris(3,5-dimethylphenylcarbamate), chitin bis(3-chloro-4-methylphenylcarbamate) with good stability and good chiral recognition capability was blended with the amylose derivative at different ratios, and the resulting blends were further coated onto 3-aminopropyl silica gel to obtain three biselector CSPs.
26460376	6	56	from	composition	1117:1127	arg1	CSPs					1257:1260	the biselector CSPs	1242:1260	the biselector CSPs	1242:1260	In addition, the influence of composition and interaction of amylose tris(3,5-dimethylphenylcarbamate) and chitin bis(3-chloro-4-methylphenylcarbamate) in the biselector CSPs on the chiral recognition and the elute orders of enantiomers was also discussed in detail.
26460376	3	57	theme	chiral	500:505	arg1	capability					519:528	the chiral recognition capability	496:528	the chiral recognition capability of amylose tris(3,5-dimethylphenylcarbamate)	496:573	In order to overcome the defect as well as maintain the chiral recognition capability of amylose tris(3,5-dimethylphenylcarbamate), chitin bis(3-chloro-4-methylphenylcarbamate) with good stability and good chiral recognition capability was blended with the amylose derivative at different ratios, and the resulting blends were further coated onto 3-aminopropyl silica gel to obtain three biselector CSPs.
26460376	3	58	theme	silica	805:810	arg1	gel					812:814	3-aminopropyl silica gel	791:814	3-aminopropyl silica gel	791:814	In order to overcome the defect as well as maintain the chiral recognition capability of amylose tris(3,5-dimethylphenylcarbamate), chitin bis(3-chloro-4-methylphenylcarbamate) with good stability and good chiral recognition capability was blended with the amylose derivative at different ratios, and the resulting blends were further coated onto 3-aminopropyl silica gel to obtain three biselector CSPs.
29122714	5	0	dep	demonstrated	735:746	arg1	influenced					874:883	influenced	874:883	were extremely influenced by polymer-blend (ALG:DMP) ratios, low density additives (olive oil/magnesium stearate) and FG-ALG coating inclusion	859:1000	Various formulations demonstrated excellent drug encapsulation efficiency (DEE, 51-70%) and sustained drug eluting behavior (Q8h, 72-96%), which were extremely influenced by polymer-blend (ALG:DMP) ratios, low density additives (olive oil/magnesium stearate) and FG-ALG coating inclusion.
29122714	3	1	theme	ionotropic	509:518	arg1	technique					529:537	ionotropic gelation technique	509:537	ionotropic gelation technique employing zinc acetate as cross-linker	509:576	MFM-loaded core matrices were then fabricated by ionotropic gelation technique employing zinc acetate as cross-linker.
29122714	1	2	theme	HCl	285:287	arg1	delivery					263:270	controlled intragastric delivery	239:270	controlled intragastric delivery of metformin HCl (MFM)	239:293	The current study aimed at developing diethonolamine-modified high-methoxyl pectin (DMP)-alginate (ALG) based core-shell composites for controlled intragastric delivery of metformin HCl (MFM) by combined approach of floating and bioadhesion.
29122714	5	3	theme	excellent	748:756	arg1	efficiency					777:786	excellent drug encapsulation efficiency	748:786	excellent drug encapsulation efficiency (DEE, 51-70%)	748:800	Various formulations demonstrated excellent drug encapsulation efficiency (DEE, 51-70%) and sustained drug eluting behavior (Q8h, 72-96%), which were extremely influenced by polymer-blend (ALG:DMP) ratios, low density additives (olive oil/magnesium stearate) and FG-ALG coating inclusion.
29122714	6	4	theme	core-shell	1035:1044	arg1	F-7					1056:1058	F-7	1056:1058	F-7	1056:1058	The drug release profile of the core-shell matrices (F-7) was best fitted in zero-order kinetic model with case-II transport driven mechanism.
29122714	6	4	theme	core-shell	1035:1044	arg1	matrices					1046:1053	the core-shell matrices	1031:1053	the core-shell matrices (F-7)	1031:1059	The drug release profile of the core-shell matrices (F-7) was best fitted in zero-order kinetic model with case-II transport driven mechanism.
29122714	1	5	theme	-alginate	191:199	arg1	composites					224:233	diethonolamine-modified high-methoxyl pectin (DMP)-alginate (ALG) based core-shell composites	141:233	diethonolamine-modified high-methoxyl pectin (DMP)-alginate (ALG) based core-shell composites for controlled intragastric delivery of metformin HCl (MFM)	141:293	The current study aimed at developing diethonolamine-modified high-methoxyl pectin (DMP)-alginate (ALG) based core-shell composites for controlled intragastric delivery of metformin HCl (MFM) by combined approach of floating and bioadhesion.
29122714	9	6	theme	type	1443:1446	arg1	diabetes					1450:1457	type 2 diabetes	1443:1457	type 2 diabetes management	1443:1468	Thus, the developed composites are appropriate for controlled stomach-specific delivery of MFM for type 2 diabetes management.
29122714	6	7	theme	release	1012:1018	arg1	profile					1020:1026	The drug release profile	1003:1026	The drug release profile of the core-shell matrices (F-7)	1003:1059	The drug release profile of the core-shell matrices (F-7) was best fitted in zero-order kinetic model with case-II transport driven mechanism.
29122714	6	8	with	model	1099:1103	arg1	mechanism					1135:1143	case-II transport driven mechanism	1110:1143	case-II transport driven mechanism	1110:1143	The drug release profile of the core-shell matrices (F-7) was best fitted in zero-order kinetic model with case-II transport driven mechanism.
29122714	5	9	theme	olive	943:947	arg1	additives					932:940	low density additives	920:940	low density additives (olive oil/magnesium stearate)	920:971	Various formulations demonstrated excellent drug encapsulation efficiency (DEE, 51-70%) and sustained drug eluting behavior (Q8h, 72-96%), which were extremely influenced by polymer-blend (ALG:DMP) ratios, low density additives (olive oil/magnesium stearate) and FG-ALG coating inclusion.
29122714	5	9	theme	olive	943:947	arg1	stearate					963:970	olive oil/magnesium stearate	943:970	olive oil/magnesium stearate	943:970	Various formulations demonstrated excellent drug encapsulation efficiency (DEE, 51-70%) and sustained drug eluting behavior (Q8h, 72-96%), which were extremely influenced by polymer-blend (ALG:DMP) ratios, low density additives (olive oil/magnesium stearate) and FG-ALG coating inclusion.
29122714	6	10	theme	case-II	1110:1116	arg1	mechanism					1135:1143	case-II transport driven mechanism	1110:1143	case-II transport driven mechanism	1110:1143	The drug release profile of the core-shell matrices (F-7) was best fitted in zero-order kinetic model with case-II transport driven mechanism.
29122714	5	11	theme	low	920:922	arg1	additives					932:940	low density additives	920:940	low density additives (olive oil/magnesium stearate)	920:971	Various formulations demonstrated excellent drug encapsulation efficiency (DEE, 51-70%) and sustained drug eluting behavior (Q8h, 72-96%), which were extremely influenced by polymer-blend (ALG:DMP) ratios, low density additives (olive oil/magnesium stearate) and FG-ALG coating inclusion.
29122714	5	11	theme	low	920:922	arg1	stearate					963:970	olive oil/magnesium stearate	943:970	olive oil/magnesium stearate	943:970	Various formulations demonstrated excellent drug encapsulation efficiency (DEE, 51-70%) and sustained drug eluting behavior (Q8h, 72-96%), which were extremely influenced by polymer-blend (ALG:DMP) ratios, low density additives (olive oil/magnesium stearate) and FG-ALG coating inclusion.
29122714	6	12	theme	driven	1128:1133	arg1	mechanism					1135:1143	case-II transport driven mechanism	1110:1143	case-II transport driven mechanism	1110:1143	The drug release profile of the core-shell matrices (F-7) was best fitted in zero-order kinetic model with case-II transport driven mechanism.
29122714	5	13	theme	oil/magnesium	949:961	arg1	additives					932:940	low density additives	920:940	low density additives (olive oil/magnesium stearate)	920:971	Various formulations demonstrated excellent drug encapsulation efficiency (DEE, 51-70%) and sustained drug eluting behavior (Q8h, 72-96%), which were extremely influenced by polymer-blend (ALG:DMP) ratios, low density additives (olive oil/magnesium stearate) and FG-ALG coating inclusion.
29122714	5	13	theme	oil/magnesium	949:961	arg1	stearate					963:970	olive oil/magnesium stearate	943:970	olive oil/magnesium stearate	943:970	Various formulations demonstrated excellent drug encapsulation efficiency (DEE, 51-70%) and sustained drug eluting behavior (Q8h, 72-96%), which were extremely influenced by polymer-blend (ALG:DMP) ratios, low density additives (olive oil/magnesium stearate) and FG-ALG coating inclusion.
29122714	6	14	theme	zero-order	1080:1089	arg1	model					1099:1103	zero-order kinetic model	1080:1103	zero-order kinetic model with case-II transport driven mechanism	1080:1143	The drug release profile of the core-shell matrices (F-7) was best fitted in zero-order kinetic model with case-II transport driven mechanism.
29122714	7	15	theme	outstanding	1164:1174	arg1	characteristics					1192:1206	outstanding gastroretentive characteristics	1164:1206	outstanding gastroretentive characteristics	1164:1206	It also portrayed outstanding gastroretentive characteristics.
29122714	8	16	theme	thermal	1303:1309	arg1	behavior					1311:1318	thermal behavior	1303:1318	thermal behavior	1303:1318	Moreover, the composites were analyzed for surface morphology, drug-excipients compatibility, thermal behavior and drug crystallinity.
29122714	3	17	theme	zinc	549:552	arg1	acetate					554:560	zinc acetate	549:560	zinc acetate	549:560	MFM-loaded core matrices were then fabricated by ionotropic gelation technique employing zinc acetate as cross-linker.
29122714	4	18	theme	diffusion-controlled	660:679	arg1	complexation					693:704	diffusion-controlled interfacial complexation	660:704	diffusion-controlled interfacial complexation method	660:711	The core matrices were further coated by fenugreek gum (FG)-ALG gel membrane via diffusion-controlled interfacial complexation method.
29122714	7	19	theme	gastroretentive	1176:1190	arg1	characteristics					1192:1206	outstanding gastroretentive characteristics	1164:1206	outstanding gastroretentive characteristics	1164:1206	It also portrayed outstanding gastroretentive characteristics.
29122714	5	20	dep	behavior	829:836	arg1	Q8h					839:841	Q8h	839:841	Q8h	839:841	Various formulations demonstrated excellent drug encapsulation efficiency (DEE, 51-70%) and sustained drug eluting behavior (Q8h, 72-96%), which were extremely influenced by polymer-blend (ALG:DMP) ratios, low density additives (olive oil/magnesium stearate) and FG-ALG coating inclusion.
29122714	5	20	dep	behavior	829:836	arg1	%					849:849	72-96%	844:849	72-96%	844:849	Various formulations demonstrated excellent drug encapsulation efficiency (DEE, 51-70%) and sustained drug eluting behavior (Q8h, 72-96%), which were extremely influenced by polymer-blend (ALG:DMP) ratios, low density additives (olive oil/magnesium stearate) and FG-ALG coating inclusion.
29122714	0	21	theme	Diethanolamine-modified	0:22	arg1	pectin					24:29	Diethanolamine-modified pectin	0:29	Diethanolamine-modified pectin	0:29	Diethanolamine-modified pectin based core-shell composites as dual working gastroretentive drug-cargo.
29122714	9	22	theme	stomach-specific	1406:1421	arg1	delivery					1423:1430	controlled stomach-specific delivery	1395:1430	controlled stomach-specific delivery of MFM for type 2 diabetes management	1395:1468	Thus, the developed composites are appropriate for controlled stomach-specific delivery of MFM for type 2 diabetes management.
29122714	6	23	theme	kinetic	1091:1097	arg1	model					1099:1103	zero-order kinetic model	1080:1103	zero-order kinetic model with case-II transport driven mechanism	1080:1143	The drug release profile of the core-shell matrices (F-7) was best fitted in zero-order kinetic model with case-II transport driven mechanism.
29122714	1	24	theme	current	107:113	arg1	study					115:119	The current study	103:119	The current study	103:119	The current study aimed at developing diethonolamine-modified high-methoxyl pectin (DMP)-alginate (ALG) based core-shell composites for controlled intragastric delivery of metformin HCl (MFM) by combined approach of floating and bioadhesion.
29122714	8	25	theme	surface	1252:1258	arg1	morphology					1260:1269	surface morphology	1252:1269	surface morphology	1252:1269	Moreover, the composites were analyzed for surface morphology, drug-excipients compatibility, thermal behavior and drug crystallinity.
29122714	2	26	theme	XRD	446:448	arg1	analyses					450:457	FTIR, DSC and XRD analyses	432:457	FTIR, DSC and XRD analyses	432:457	DMP with degree of amidation of 48.72% was initially accomplished and characterized by FTIR, DSC and XRD analyses.
29122714	5	27	dep	ALG	903:905	arg1	DMP					907:909	DMP	907:909	ALG:DMP	903:909	Various formulations demonstrated excellent drug encapsulation efficiency (DEE, 51-70%) and sustained drug eluting behavior (Q8h, 72-96%), which were extremely influenced by polymer-blend (ALG:DMP) ratios, low density additives (olive oil/magnesium stearate) and FG-ALG coating inclusion.
29122714	5	28	theme	density	924:930	arg1	additives					932:940	low density additives	920:940	low density additives (olive oil/magnesium stearate)	920:971	Various formulations demonstrated excellent drug encapsulation efficiency (DEE, 51-70%) and sustained drug eluting behavior (Q8h, 72-96%), which were extremely influenced by polymer-blend (ALG:DMP) ratios, low density additives (olive oil/magnesium stearate) and FG-ALG coating inclusion.
29122714	5	28	theme	density	924:930	arg1	stearate					963:970	olive oil/magnesium stearate	943:970	olive oil/magnesium stearate	943:970	Various formulations demonstrated excellent drug encapsulation efficiency (DEE, 51-70%) and sustained drug eluting behavior (Q8h, 72-96%), which were extremely influenced by polymer-blend (ALG:DMP) ratios, low density additives (olive oil/magnesium stearate) and FG-ALG coating inclusion.
29122714	1	29	theme	based	207:211	arg1	composites					224:233	diethonolamine-modified high-methoxyl pectin (DMP)-alginate (ALG) based core-shell composites	141:233	diethonolamine-modified high-methoxyl pectin (DMP)-alginate (ALG) based core-shell composites for controlled intragastric delivery of metformin HCl (MFM)	141:293	The current study aimed at developing diethonolamine-modified high-methoxyl pectin (DMP)-alginate (ALG) based core-shell composites for controlled intragastric delivery of metformin HCl (MFM) by combined approach of floating and bioadhesion.
29122714	0	30	theme	core-shell	37:46	arg1	composites					48:57	core-shell composites	37:57	core-shell composites	37:57	Diethanolamine-modified pectin based core-shell composites as dual working gastroretentive drug-cargo.
29122714	1	31	theme	combined	298:305	arg1	approach					307:314	combined approach	298:314	combined approach of floating and bioadhesion	298:342	The current study aimed at developing diethonolamine-modified high-methoxyl pectin (DMP)-alginate (ALG) based core-shell composites for controlled intragastric delivery of metformin HCl (MFM) by combined approach of floating and bioadhesion.
29122714	5	32	theme	eluting	821:827	arg1	behavior					829:836	sustained drug eluting behavior	806:836	sustained drug eluting behavior (Q8h, 72-96%)	806:850	Various formulations demonstrated excellent drug encapsulation efficiency (DEE, 51-70%) and sustained drug eluting behavior (Q8h, 72-96%), which were extremely influenced by polymer-blend (ALG:DMP) ratios, low density additives (olive oil/magnesium stearate) and FG-ALG coating inclusion.
29122714	1	33	theme	core-shell	213:222	arg1	composites					224:233	diethonolamine-modified high-methoxyl pectin (DMP)-alginate (ALG) based core-shell composites	141:233	diethonolamine-modified high-methoxyl pectin (DMP)-alginate (ALG) based core-shell composites for controlled intragastric delivery of metformin HCl (MFM)	141:293	The current study aimed at developing diethonolamine-modified high-methoxyl pectin (DMP)-alginate (ALG) based core-shell composites for controlled intragastric delivery of metformin HCl (MFM) by combined approach of floating and bioadhesion.
29122714	2	34	theme	DSC	438:440	arg1	analyses					450:457	FTIR, DSC and XRD analyses	432:457	FTIR, DSC and XRD analyses	432:457	DMP with degree of amidation of 48.72% was initially accomplished and characterized by FTIR, DSC and XRD analyses.
29122714	9	35	theme	developed	1354:1362	arg1	appropriate					1379:1389	appropriate	1379:1389	appropriate	1379:1389	Thus, the developed composites are appropriate for controlled stomach-specific delivery of MFM for type 2 diabetes management.
29122714	9	35	theme	developed	1354:1362	arg1	composites					1364:1373	the developed composites	1350:1373	the developed composites	1350:1373	Thus, the developed composites are appropriate for controlled stomach-specific delivery of MFM for type 2 diabetes management.
29122714	4	36	theme	gum	630:632	arg1	membrane					647:654	fenugreek gum (FG)-ALG gel membrane	620:654	fenugreek gum (FG)-ALG gel membrane	620:654	The core matrices were further coated by fenugreek gum (FG)-ALG gel membrane via diffusion-controlled interfacial complexation method.
29122714	2	37	theme	amidation	364:372	arg1	degree					354:359	degree	354:359	degree of amidation of 48.72%	354:382	DMP with degree of amidation of 48.72% was initially accomplished and characterized by FTIR, DSC and XRD analyses.
29122714	9	38	theme	diabetes	1450:1457	arg1	management					1459:1468	type 2 diabetes management	1443:1468	type 2 diabetes management	1443:1468	Thus, the developed composites are appropriate for controlled stomach-specific delivery of MFM for type 2 diabetes management.
29122714	2	39	theme	FTIR	432:435	arg1	analyses					450:457	FTIR, DSC and XRD analyses	432:457	FTIR, DSC and XRD analyses	432:457	DMP with degree of amidation of 48.72% was initially accomplished and characterized by FTIR, DSC and XRD analyses.
29122714	5	40	theme	sustained	806:814	arg1	behavior					829:836	sustained drug eluting behavior	806:836	sustained drug eluting behavior (Q8h, 72-96%)	806:850	Various formulations demonstrated excellent drug encapsulation efficiency (DEE, 51-70%) and sustained drug eluting behavior (Q8h, 72-96%), which were extremely influenced by polymer-blend (ALG:DMP) ratios, low density additives (olive oil/magnesium stearate) and FG-ALG coating inclusion.
29122714	4	41	theme	core	583:586	arg1	matrices					588:595	The core matrices	579:595	The core matrices	579:595	The core matrices were further coated by fenugreek gum (FG)-ALG gel membrane via diffusion-controlled interfacial complexation method.
29122714	5	42	theme	drug	758:761	arg1	efficiency					777:786	excellent drug encapsulation efficiency	748:786	excellent drug encapsulation efficiency (DEE, 51-70%)	748:800	Various formulations demonstrated excellent drug encapsulation efficiency (DEE, 51-70%) and sustained drug eluting behavior (Q8h, 72-96%), which were extremely influenced by polymer-blend (ALG:DMP) ratios, low density additives (olive oil/magnesium stearate) and FG-ALG coating inclusion.
29122714	2	43	with	DMP	345:347	arg1	degree					354:359	degree	354:359	degree of amidation of 48.72%	354:382	DMP with degree of amidation of 48.72% was initially accomplished and characterized by FTIR, DSC and XRD analyses.
29122714	4	44	theme	gel	643:645	arg1	membrane					647:654	fenugreek gum (FG)-ALG gel membrane	620:654	fenugreek gum (FG)-ALG gel membrane	620:654	The core matrices were further coated by fenugreek gum (FG)-ALG gel membrane via diffusion-controlled interfacial complexation method.
29122714	4	45	theme	interfacial	681:691	arg1	complexation					693:704	diffusion-controlled interfacial complexation	660:704	diffusion-controlled interfacial complexation method	660:711	The core matrices were further coated by fenugreek gum (FG)-ALG gel membrane via diffusion-controlled interfacial complexation method.
29122714	5	46	theme	drug	816:819	arg1	behavior					829:836	sustained drug eluting behavior	806:836	sustained drug eluting behavior (Q8h, 72-96%)	806:850	Various formulations demonstrated excellent drug encapsulation efficiency (DEE, 51-70%) and sustained drug eluting behavior (Q8h, 72-96%), which were extremely influenced by polymer-blend (ALG:DMP) ratios, low density additives (olive oil/magnesium stearate) and FG-ALG coating inclusion.
29122714	1	47	theme	floating	319:326	arg1	approach					307:314	combined approach	298:314	combined approach of floating and bioadhesion	298:342	The current study aimed at developing diethonolamine-modified high-methoxyl pectin (DMP)-alginate (ALG) based core-shell composites for controlled intragastric delivery of metformin HCl (MFM) by combined approach of floating and bioadhesion.
29122714	5	48	theme	encapsulation	763:775	arg1	efficiency					777:786	excellent drug encapsulation efficiency	748:786	excellent drug encapsulation efficiency (DEE, 51-70%)	748:800	Various formulations demonstrated excellent drug encapsulation efficiency (DEE, 51-70%) and sustained drug eluting behavior (Q8h, 72-96%), which were extremely influenced by polymer-blend (ALG:DMP) ratios, low density additives (olive oil/magnesium stearate) and FG-ALG coating inclusion.
29122714	1	49	theme	controlled	239:248	arg1	delivery					263:270	controlled intragastric delivery	239:270	controlled intragastric delivery of metformin HCl (MFM)	239:293	The current study aimed at developing diethonolamine-modified high-methoxyl pectin (DMP)-alginate (ALG) based core-shell composites for controlled intragastric delivery of metformin HCl (MFM) by combined approach of floating and bioadhesion.
29122714	0	50	theme	working	67:73	arg1	drug-cargo					91:100	dual working gastroretentive drug-cargo	62:100	dual working gastroretentive drug-cargo	62:100	Diethanolamine-modified pectin based core-shell composites as dual working gastroretentive drug-cargo.
29122714	5	51	theme	polymer-blend	888:900	arg1	ALG					903:905	ALG	903:905	ALG:DMP	903:909	Various formulations demonstrated excellent drug encapsulation efficiency (DEE, 51-70%) and sustained drug eluting behavior (Q8h, 72-96%), which were extremely influenced by polymer-blend (ALG:DMP) ratios, low density additives (olive oil/magnesium stearate) and FG-ALG coating inclusion.
29122714	5	51	theme	polymer-blend	888:900	arg1	ratios					912:917	polymer-blend (ALG:DMP) ratios	888:917	polymer-blend (ALG:DMP) ratios	888:917	Various formulations demonstrated excellent drug encapsulation efficiency (DEE, 51-70%) and sustained drug eluting behavior (Q8h, 72-96%), which were extremely influenced by polymer-blend (ALG:DMP) ratios, low density additives (olive oil/magnesium stearate) and FG-ALG coating inclusion.
29122714	5	52	dep	efficiency	777:786	arg1	DEE					789:791	DEE	789:791	DEE	789:791	Various formulations demonstrated excellent drug encapsulation efficiency (DEE, 51-70%) and sustained drug eluting behavior (Q8h, 72-96%), which were extremely influenced by polymer-blend (ALG:DMP) ratios, low density additives (olive oil/magnesium stearate) and FG-ALG coating inclusion.
29122714	5	52	dep	efficiency	777:786	arg1	%					799:799	51-70%	794:799	51-70%	794:799	Various formulations demonstrated excellent drug encapsulation efficiency (DEE, 51-70%) and sustained drug eluting behavior (Q8h, 72-96%), which were extremely influenced by polymer-blend (ALG:DMP) ratios, low density additives (olive oil/magnesium stearate) and FG-ALG coating inclusion.
29122714	8	53	theme	drug	1324:1327	arg1	crystallinity					1329:1341	drug crystallinity	1324:1341	drug crystallinity	1324:1341	Moreover, the composites were analyzed for surface morphology, drug-excipients compatibility, thermal behavior and drug crystallinity.
29122714	0	54	theme	dual	62:65	arg1	drug-cargo					91:100	dual working gastroretentive drug-cargo	62:100	dual working gastroretentive drug-cargo	62:100	Diethanolamine-modified pectin based core-shell composites as dual working gastroretentive drug-cargo.
29122714	1	55	theme	bioadhesion	332:342	arg1	approach					307:314	combined approach	298:314	combined approach of floating and bioadhesion	298:342	The current study aimed at developing diethonolamine-modified high-methoxyl pectin (DMP)-alginate (ALG) based core-shell composites for controlled intragastric delivery of metformin HCl (MFM) by combined approach of floating and bioadhesion.
29122714	3	56	theme	gelation	520:527	arg1	technique					529:537	ionotropic gelation technique	509:537	ionotropic gelation technique employing zinc acetate as cross-linker	509:576	MFM-loaded core matrices were then fabricated by ionotropic gelation technique employing zinc acetate as cross-linker.
29122714	1	57	theme	diethonolamine-modified	141:163	arg1	ALG					202:204	ALG	202:204	ALG	202:204	The current study aimed at developing diethonolamine-modified high-methoxyl pectin (DMP)-alginate (ALG) based core-shell composites for controlled intragastric delivery of metformin HCl (MFM) by combined approach of floating and bioadhesion.
29122714	1	57	theme	diethonolamine-modified	141:163	arg1	-alginate					191:199	diethonolamine-modified high-methoxyl pectin (DMP)-alginate	141:199	diethonolamine-modified high-methoxyl pectin (DMP)-alginate (ALG) based core-shell composites for controlled intragastric delivery of metformin HCl (MFM)	141:293	The current study aimed at developing diethonolamine-modified high-methoxyl pectin (DMP)-alginate (ALG) based core-shell composites for controlled intragastric delivery of metformin HCl (MFM) by combined approach of floating and bioadhesion.
29122714	9	58	theme	controlled	1395:1404	arg1	delivery					1423:1430	controlled stomach-specific delivery	1395:1430	controlled stomach-specific delivery of MFM for type 2 diabetes management	1395:1468	Thus, the developed composites are appropriate for controlled stomach-specific delivery of MFM for type 2 diabetes management.
29122714	5	59	theme	Various	714:720	arg1	formulations					722:733	Various formulations	714:733	Various formulations	714:733	Various formulations demonstrated excellent drug encapsulation efficiency (DEE, 51-70%) and sustained drug eluting behavior (Q8h, 72-96%), which were extremely influenced by polymer-blend (ALG:DMP) ratios, low density additives (olive oil/magnesium stearate) and FG-ALG coating inclusion.
29122714	4	60	dep	complexation	693:704	arg1	method					706:711	method	706:711	diffusion-controlled interfacial complexation method	660:711	The core matrices were further coated by fenugreek gum (FG)-ALG gel membrane via diffusion-controlled interfacial complexation method.
29122714	6	61	theme	drug	1007:1010	arg1	profile					1020:1026	The drug release profile	1003:1026	The drug release profile of the core-shell matrices (F-7)	1003:1059	The drug release profile of the core-shell matrices (F-7) was best fitted in zero-order kinetic model with case-II transport driven mechanism.
29122714	1	62	theme	high-methoxyl	165:177	arg1	ALG					202:204	ALG	202:204	ALG	202:204	The current study aimed at developing diethonolamine-modified high-methoxyl pectin (DMP)-alginate (ALG) based core-shell composites for controlled intragastric delivery of metformin HCl (MFM) by combined approach of floating and bioadhesion.
29122714	1	62	theme	high-methoxyl	165:177	arg1	-alginate					191:199	diethonolamine-modified high-methoxyl pectin (DMP)-alginate	141:199	diethonolamine-modified high-methoxyl pectin (DMP)-alginate (ALG) based core-shell composites for controlled intragastric delivery of metformin HCl (MFM)	141:293	The current study aimed at developing diethonolamine-modified high-methoxyl pectin (DMP)-alginate (ALG) based core-shell composites for controlled intragastric delivery of metformin HCl (MFM) by combined approach of floating and bioadhesion.
29122714	6	63	theme	transport	1118:1126	arg1	mechanism					1135:1143	case-II transport driven mechanism	1110:1143	case-II transport driven mechanism	1110:1143	The drug release profile of the core-shell matrices (F-7) was best fitted in zero-order kinetic model with case-II transport driven mechanism.
29122714	1	64	theme	intragastric	250:261	arg1	delivery					263:270	controlled intragastric delivery	239:270	controlled intragastric delivery of metformin HCl (MFM)	239:293	The current study aimed at developing diethonolamine-modified high-methoxyl pectin (DMP)-alginate (ALG) based core-shell composites for controlled intragastric delivery of metformin HCl (MFM) by combined approach of floating and bioadhesion.
29122714	6	65	theme	matrices	1046:1053	arg1	profile					1020:1026	The drug release profile	1003:1026	The drug release profile of the core-shell matrices (F-7)	1003:1059	The drug release profile of the core-shell matrices (F-7) was best fitted in zero-order kinetic model with case-II transport driven mechanism.
29122714	1	66	theme	pectin	179:184	arg1	ALG					202:204	ALG	202:204	ALG	202:204	The current study aimed at developing diethonolamine-modified high-methoxyl pectin (DMP)-alginate (ALG) based core-shell composites for controlled intragastric delivery of metformin HCl (MFM) by combined approach of floating and bioadhesion.
29122714	1	66	theme	pectin	179:184	arg1	-alginate					191:199	diethonolamine-modified high-methoxyl pectin (DMP)-alginate	141:199	diethonolamine-modified high-methoxyl pectin (DMP)-alginate (ALG) based core-shell composites for controlled intragastric delivery of metformin HCl (MFM)	141:293	The current study aimed at developing diethonolamine-modified high-methoxyl pectin (DMP)-alginate (ALG) based core-shell composites for controlled intragastric delivery of metformin HCl (MFM) by combined approach of floating and bioadhesion.
29122714	4	67	theme	fenugreek	620:628	arg1	membrane					647:654	fenugreek gum (FG)-ALG gel membrane	620:654	fenugreek gum (FG)-ALG gel membrane	620:654	The core matrices were further coated by fenugreek gum (FG)-ALG gel membrane via diffusion-controlled interfacial complexation method.
29122714	3	68	theme	MFM-loaded	460:469	arg1	matrices					476:483	MFM-loaded core matrices	460:483	MFM-loaded core matrices	460:483	MFM-loaded core matrices were then fabricated by ionotropic gelation technique employing zinc acetate as cross-linker.
29122714	8	69	theme	drug-excipients	1272:1286	arg1	compatibility					1288:1300	drug-excipients compatibility	1272:1300	drug-excipients compatibility	1272:1300	Moreover, the composites were analyzed for surface morphology, drug-excipients compatibility, thermal behavior and drug crystallinity.
29122714	3	70	theme	core	471:474	arg1	matrices					476:483	MFM-loaded core matrices	460:483	MFM-loaded core matrices	460:483	MFM-loaded core matrices were then fabricated by ionotropic gelation technique employing zinc acetate as cross-linker.
29122714	2	71	theme	%	382:382	arg1	amidation					364:372	amidation	364:372	amidation of 48.72%	364:382	DMP with degree of amidation of 48.72% was initially accomplished and characterized by FTIR, DSC and XRD analyses.
29122714	4	72	theme	FG	635:636	arg1	membrane					647:654	fenugreek gum (FG)-ALG gel membrane	620:654	fenugreek gum (FG)-ALG gel membrane	620:654	The core matrices were further coated by fenugreek gum (FG)-ALG gel membrane via diffusion-controlled interfacial complexation method.
29122714	0	73	theme	gastroretentive	75:89	arg1	drug-cargo					91:100	dual working gastroretentive drug-cargo	62:100	dual working gastroretentive drug-cargo	62:100	Diethanolamine-modified pectin based core-shell composites as dual working gastroretentive drug-cargo.
29122714	4	74	theme	-ALG	638:641	arg1	membrane					647:654	fenugreek gum (FG)-ALG gel membrane	620:654	fenugreek gum (FG)-ALG gel membrane	620:654	The core matrices were further coated by fenugreek gum (FG)-ALG gel membrane via diffusion-controlled interfacial complexation method.
29122714	1	75	theme	DMP	187:189	arg1	ALG					202:204	ALG	202:204	ALG	202:204	The current study aimed at developing diethonolamine-modified high-methoxyl pectin (DMP)-alginate (ALG) based core-shell composites for controlled intragastric delivery of metformin HCl (MFM) by combined approach of floating and bioadhesion.
29122714	1	75	theme	DMP	187:189	arg1	-alginate					191:199	diethonolamine-modified high-methoxyl pectin (DMP)-alginate	141:199	diethonolamine-modified high-methoxyl pectin (DMP)-alginate (ALG) based core-shell composites for controlled intragastric delivery of metformin HCl (MFM)	141:293	The current study aimed at developing diethonolamine-modified high-methoxyl pectin (DMP)-alginate (ALG) based core-shell composites for controlled intragastric delivery of metformin HCl (MFM) by combined approach of floating and bioadhesion.
29122714	9	76	theme	MFM	1435:1437	arg1	delivery					1423:1430	controlled stomach-specific delivery	1395:1430	controlled stomach-specific delivery of MFM for type 2 diabetes management	1395:1468	Thus, the developed composites are appropriate for controlled stomach-specific delivery of MFM for type 2 diabetes management.
29122714	1	77	theme	metformin	275:283	arg1	MFM					290:292	MFM	290:292	MFM	290:292	The current study aimed at developing diethonolamine-modified high-methoxyl pectin (DMP)-alginate (ALG) based core-shell composites for controlled intragastric delivery of metformin HCl (MFM) by combined approach of floating and bioadhesion.
29122714	1	77	theme	metformin	275:283	arg1	HCl					285:287	metformin HCl	275:287	metformin HCl (MFM)	275:293	The current study aimed at developing diethonolamine-modified high-methoxyl pectin (DMP)-alginate (ALG) based core-shell composites for controlled intragastric delivery of metformin HCl (MFM) by combined approach of floating and bioadhesion.
26306633	7	0	theme	analytical	1365:1374	arg1	approach					1376:1383	the standard analytical approach	1352:1383	the standard analytical approach	1352:1383	N-Glycoprotein analysis of B. mori fifth instar larvae revealed that high-mannose-type structure was predominant and GlcNAc-linked and fucosylated structures were observed but endogenous galactosyl-, N-acetylgalactosaminyl- and sialyl-N-glycoproteins were undetectable under the standard analytical approach.
26306633	8	1	theme	sialylation	1498:1508	arg1	potentials					1510:1519	the sialylation potentials	1494:1519	the sialylation potentials	1494:1519	These results indicate that B. mori genome encodes an α2,6-sialyltransferase, but further investigations of the sialylation potentials are necessary.
26306633	7	2	gly	fucosylated	1212:1222	arg1	structures					1224:1233	fucosylated structures	1212:1233	fucosylated structures	1212:1233	N-Glycoprotein analysis of B. mori fifth instar larvae revealed that high-mannose-type structure was predominant and GlcNAc-linked and fucosylated structures were observed but endogenous galactosyl-, N-acetylgalactosaminyl- and sialyl-N-glycoproteins were undetectable under the standard analytical approach.
26306633	0	3	theme	active	74:79	arg1	α2,6-sialyltransferase					81:102	an active α2,6-sialyltransferase	71:102	an active α2,6-sialyltransferase	71:102	Sialylation potentials of the silkworm, Bombyx mori; B. mori possesses an active α2,6-sialyltransferase.
26306633	7	4	gly	sialyl-N-glycoproteins	1305:1326	arg1	sialyl-N-glycoproteins					1305:1326	sialyl-N-glycoproteins	1305:1326	sialyl-N-glycoproteins	1305:1326	N-Glycoprotein analysis of B. mori fifth instar larvae revealed that high-mannose-type structure was predominant and GlcNAc-linked and fucosylated structures were observed but endogenous galactosyl-, N-acetylgalactosaminyl- and sialyl-N-glycoproteins were undetectable under the standard analytical approach.
26306633	7	5	theme	fucosylated	1212:1222	arg1	structures					1224:1233	fucosylated structures	1212:1233	fucosylated structures	1212:1233	N-Glycoprotein analysis of B. mori fifth instar larvae revealed that high-mannose-type structure was predominant and GlcNAc-linked and fucosylated structures were observed but endogenous galactosyl-, N-acetylgalactosaminyl- and sialyl-N-glycoproteins were undetectable under the standard analytical approach.
26306633	3	6	theme	Golgi-localized	506:520	arg1	which					495:499	which	495:499	which	495:499	Here, we identified Bombyx mori sialyltransferase (BmST), which is a Golgi-localized glycosyltransferase and which can modify N-glycans.
26306633	3	6	theme	Golgi-localized	506:520	arg1	glycosyltransferase					522:540	a Golgi-localized glycosyltransferase	504:540	a Golgi-localized glycosyltransferase	504:540	Here, we identified Bombyx mori sialyltransferase (BmST), which is a Golgi-localized glycosyltransferase and which can modify N-glycans.
26306633	4	7	theme	different	609:617	arg1	organs					619:624	different organs	609:624	different organs	609:624	BmST was ubiquitously expressed in different organs and in various stages of development and localized at the Golgi.
26306633	7	8	theme	standard	1356:1363	arg1	approach					1376:1383	the standard analytical approach	1352:1383	the standard analytical approach	1352:1383	N-Glycoprotein analysis of B. mori fifth instar larvae revealed that high-mannose-type structure was predominant and GlcNAc-linked and fucosylated structures were observed but endogenous galactosyl-, N-acetylgalactosaminyl- and sialyl-N-glycoproteins were undetectable under the standard analytical approach.
26306633	8	9	theme	potentials	1510:1519	arg1	investigations					1476:1489	further investigations	1468:1489	further investigations of the sialylation potentials	1468:1519	These results indicate that B. mori genome encodes an α2,6-sialyltransferase, but further investigations of the sialylation potentials are necessary.
26306633	5	10	theme	highest	893:899	arg1	activity					901:908	the highest activity	889:908	the highest activity	889:908	Biochemical analysis using Sf9-expressed BmST revealed that BmST encoded α2,6-sialyltransferase and transferred N-acetylneuraminic acid (NeuAc) to the nonreducing terminus of Galβ1-R, but exhibited the highest activity toward GalNAcβ1,4-GlcNAc-R.
26306633	6	11	theme	human	945:949	arg1	α2,6-sialyltransferase					951:972	human α2,6-sialyltransferase	945:972	human α2,6-sialyltransferase	945:972	Unlike human α2,6-sialyltransferase, BmST required the post-translational modification, especially N-glycosylation, for its full activity.
26306633	1	12	from	modification	156:167	arg1	proteins					205:212	most secreted and membrane-bound proteins	172:212	most secreted and membrane-bound proteins in eukaryotic cells	172:232	N-Glycosylation is an important post-translational modification in most secreted and membrane-bound proteins in eukaryotic cells.
26306633	8	13	theme	B.	1414:1415	arg1	genome					1422:1427	B. mori genome	1414:1427	B. mori genome	1414:1427	These results indicate that B. mori genome encodes an α2,6-sialyltransferase, but further investigations of the sialylation potentials are necessary.
26306633	8	13	theme	B.	1414:1415	arg1	α2,6-sialyltransferase					1440:1461	an α2,6-sialyltransferase	1437:1461	an α2,6-sialyltransferase	1437:1461	These results indicate that B. mori genome encodes an α2,6-sialyltransferase, but further investigations of the sialylation potentials are necessary.
26306633	1	14	theme	secreted	177:184	arg1	proteins					205:212	most secreted and membrane-bound proteins	172:212	most secreted and membrane-bound proteins in eukaryotic cells	172:232	N-Glycosylation is an important post-translational modification in most secreted and membrane-bound proteins in eukaryotic cells.
26306633	6	15	theme	full	1062:1065	arg1	activity					1067:1074	its full activity	1058:1074	its full activity	1058:1074	Unlike human α2,6-sialyltransferase, BmST required the post-translational modification, especially N-glycosylation, for its full activity.
26306633	2	16	from	studies	375:381	arg1	subjects					392:399	these subjects	386:399	these subjects	386:399	However, the insect N-glycosylation pathway and the potentials contributing to the N-glycan synthesis are still unclear because most of the studies on these subjects have focused on mammals and plants.
26306633	7	17	theme	N-Glycoprotein	1077:1090	arg1	analysis					1092:1099	N-Glycoprotein analysis	1077:1099	N-Glycoprotein analysis of B. mori fifth instar larvae	1077:1130	N-Glycoprotein analysis of B. mori fifth instar larvae revealed that high-mannose-type structure was predominant and GlcNAc-linked and fucosylated structures were observed but endogenous galactosyl-, N-acetylgalactosaminyl- and sialyl-N-glycoproteins were undetectable under the standard analytical approach.
26306633	5	18	theme	nonreducing	842:852	arg1	terminus					854:861	the nonreducing terminus	838:861	the nonreducing terminus of Galβ1-R	838:872	Biochemical analysis using Sf9-expressed BmST revealed that BmST encoded α2,6-sialyltransferase and transferred N-acetylneuraminic acid (NeuAc) to the nonreducing terminus of Galβ1-R, but exhibited the highest activity toward GalNAcβ1,4-GlcNAc-R.
26306633	1	19	theme	membrane-bound	190:203	arg1	proteins					205:212	most secreted and membrane-bound proteins	172:212	most secreted and membrane-bound proteins in eukaryotic cells	172:232	N-Glycosylation is an important post-translational modification in most secreted and membrane-bound proteins in eukaryotic cells.
26306633	0	20	theme	Sialylation	0:10	arg1	potentials					12:21	Sialylation potentials	0:21	Sialylation potentials of the silkworm, Bombyx mori; B. mori possesses an active α2,6-sialyltransferase.	0:103	Sialylation potentials of the silkworm, Bombyx mori; B. mori possesses an active α2,6-sialyltransferase.
26306633	8	21	theme	mori	1417:1420	arg1	genome					1422:1427	B. mori genome	1414:1427	B. mori genome	1414:1427	These results indicate that B. mori genome encodes an α2,6-sialyltransferase, but further investigations of the sialylation potentials are necessary.
26306633	8	21	theme	mori	1417:1420	arg1	α2,6-sialyltransferase					1440:1461	an α2,6-sialyltransferase	1437:1461	an α2,6-sialyltransferase	1437:1461	These results indicate that B. mori genome encodes an α2,6-sialyltransferase, but further investigations of the sialylation potentials are necessary.
26306633	5	22	theme	Sf9-expressed	718:730	arg1	BmST					732:735	Sf9-expressed BmST	718:735	Sf9-expressed BmST	718:735	Biochemical analysis using Sf9-expressed BmST revealed that BmST encoded α2,6-sialyltransferase and transferred N-acetylneuraminic acid (NeuAc) to the nonreducing terminus of Galβ1-R, but exhibited the highest activity toward GalNAcβ1,4-GlcNAc-R.
26306633	0	23	dep	silkworm	30:37	arg1	Bombyx					40:45	Bombyx	40:45	Bombyx	40:45	Sialylation potentials of the silkworm, Bombyx mori; B. mori possesses an active α2,6-sialyltransferase.
26306633	1	24	from	proteins	205:212	arg1	cells					228:232	eukaryotic cells	217:232	eukaryotic cells	217:232	N-Glycosylation is an important post-translational modification in most secreted and membrane-bound proteins in eukaryotic cells.
26306633	2	25	theme	N-glycan	318:325	arg1	synthesis					327:335	the N-glycan synthesis	314:335	the N-glycan synthesis	314:335	However, the insect N-glycosylation pathway and the potentials contributing to the N-glycan synthesis are still unclear because most of the studies on these subjects have focused on mammals and plants.
26306633	1	26	theme	eukaryotic	217:226	arg1	cells					228:232	eukaryotic cells	217:232	eukaryotic cells	217:232	N-Glycosylation is an important post-translational modification in most secreted and membrane-bound proteins in eukaryotic cells.
26306633	2	27	theme	insect	248:253	arg1	pathway					271:277	the insect N-glycosylation pathway	244:277	the insect N-glycosylation pathway	244:277	However, the insect N-glycosylation pathway and the potentials contributing to the N-glycan synthesis are still unclear because most of the studies on these subjects have focused on mammals and plants.
26306633	2	27	theme	insect	248:253	arg1	unclear					347:353	unclear	347:353	unclear	347:353	However, the insect N-glycosylation pathway and the potentials contributing to the N-glycan synthesis are still unclear because most of the studies on these subjects have focused on mammals and plants.
26306633	3	28	theme	Bombyx	457:462	arg1	mori					464:467	Bombyx mori	457:467	Bombyx mori sialyltransferase (BmST)	457:492	Here, we identified Bombyx mori sialyltransferase (BmST), which is a Golgi-localized glycosyltransferase and which can modify N-glycans.
26306633	0	29	theme	silkworm	30:37	arg1	mori					47:50	the silkworm, Bombyx mori	26:50	the silkworm, Bombyx mori	26:50	Sialylation potentials of the silkworm, Bombyx mori; B. mori possesses an active α2,6-sialyltransferase.
26306633	7	30	theme	high-mannose-type	1146:1162	arg1	structure					1164:1172	high-mannose-type structure	1146:1172	high-mannose-type structure	1146:1172	N-Glycoprotein analysis of B. mori fifth instar larvae revealed that high-mannose-type structure was predominant and GlcNAc-linked and fucosylated structures were observed but endogenous galactosyl-, N-acetylgalactosaminyl- and sialyl-N-glycoproteins were undetectable under the standard analytical approach.
26306633	7	31	theme	B.	1104:1105	arg1	larvae					1125:1130	B. mori fifth instar larvae	1104:1130	B. mori fifth instar larvae	1104:1130	N-Glycoprotein analysis of B. mori fifth instar larvae revealed that high-mannose-type structure was predominant and GlcNAc-linked and fucosylated structures were observed but endogenous galactosyl-, N-acetylgalactosaminyl- and sialyl-N-glycoproteins were undetectable under the standard analytical approach.
26306633	3	32	theme	mori	464:467	arg1	BmST					488:491	BmST	488:491	BmST	488:491	Here, we identified Bombyx mori sialyltransferase (BmST), which is a Golgi-localized glycosyltransferase and which can modify N-glycans.
26306633	3	32	theme	mori	464:467	arg1	sialyltransferase					469:485	Bombyx mori sialyltransferase	457:485	Bombyx mori sialyltransferase (BmST)	457:492	Here, we identified Bombyx mori sialyltransferase (BmST), which is a Golgi-localized glycosyltransferase and which can modify N-glycans.
26306633	0	33	theme	mori	47:50	arg1	potentials					12:21	Sialylation potentials	0:21	Sialylation potentials of the silkworm, Bombyx mori; B. mori possesses an active α2,6-sialyltransferase.	0:103	Sialylation potentials of the silkworm, Bombyx mori; B. mori possesses an active α2,6-sialyltransferase.
26306633	7	34	theme	larvae	1125:1130	arg1	analysis					1092:1099	N-Glycoprotein analysis	1077:1099	N-Glycoprotein analysis of B. mori fifth instar larvae	1077:1130	N-Glycoprotein analysis of B. mori fifth instar larvae revealed that high-mannose-type structure was predominant and GlcNAc-linked and fucosylated structures were observed but endogenous galactosyl-, N-acetylgalactosaminyl- and sialyl-N-glycoproteins were undetectable under the standard analytical approach.
26306633	7	35	theme	mori	1107:1110	arg1	larvae					1125:1130	B. mori fifth instar larvae	1104:1130	B. mori fifth instar larvae	1104:1130	N-Glycoprotein analysis of B. mori fifth instar larvae revealed that high-mannose-type structure was predominant and GlcNAc-linked and fucosylated structures were observed but endogenous galactosyl-, N-acetylgalactosaminyl- and sialyl-N-glycoproteins were undetectable under the standard analytical approach.
26306633	7	36	theme	endogenous	1253:1262	arg1	galactosyl-					1264:1274	endogenous galactosyl-	1253:1274	endogenous galactosyl-	1253:1274	N-Glycoprotein analysis of B. mori fifth instar larvae revealed that high-mannose-type structure was predominant and GlcNAc-linked and fucosylated structures were observed but endogenous galactosyl-, N-acetylgalactosaminyl- and sialyl-N-glycoproteins were undetectable under the standard analytical approach.
26306633	5	37	theme	Biochemical	691:701	arg1	analysis					703:710	Biochemical analysis	691:710	Biochemical analysis using Sf9-expressed BmST	691:735	Biochemical analysis using Sf9-expressed BmST revealed that BmST encoded α2,6-sialyltransferase and transferred N-acetylneuraminic acid (NeuAc) to the nonreducing terminus of Galβ1-R, but exhibited the highest activity toward GalNAcβ1,4-GlcNAc-R.
26306633	3	38	dep	identified	446:455	arg1	which					495:499	which	495:499	which	495:499	Here, we identified Bombyx mori sialyltransferase (BmST), which is a Golgi-localized glycosyltransferase and which can modify N-glycans.
26306633	3	38	dep	identified	446:455	arg1	modify					556:561	modify	556:561	can modify N-glycans	552:571	Here, we identified Bombyx mori sialyltransferase (BmST), which is a Golgi-localized glycosyltransferase and which can modify N-glycans.
26306633	3	38	dep	identified	446:455	arg1	glycosyltransferase					522:540	a Golgi-localized glycosyltransferase	504:540	a Golgi-localized glycosyltransferase	504:540	Here, we identified Bombyx mori sialyltransferase (BmST), which is a Golgi-localized glycosyltransferase and which can modify N-glycans.
26306633	6	39	theme	post-translational	993:1010	arg1	modification					1012:1023	the post-translational modification	989:1023	the post-translational modification	989:1023	Unlike human α2,6-sialyltransferase, BmST required the post-translational modification, especially N-glycosylation, for its full activity.
26306633	6	39	theme	post-translational	993:1010	arg1	N-glycosylation					1037:1051	N-glycosylation	1037:1051	especially N-glycosylation	1026:1051	Unlike human α2,6-sialyltransferase, BmST required the post-translational modification, especially N-glycosylation, for its full activity.
26306633	7	40	theme	fifth	1112:1116	arg1	larvae					1125:1130	B. mori fifth instar larvae	1104:1130	B. mori fifth instar larvae	1104:1130	N-Glycoprotein analysis of B. mori fifth instar larvae revealed that high-mannose-type structure was predominant and GlcNAc-linked and fucosylated structures were observed but endogenous galactosyl-, N-acetylgalactosaminyl- and sialyl-N-glycoproteins were undetectable under the standard analytical approach.
26306633	8	41	theme	further	1468:1474	arg1	investigations					1476:1489	further investigations	1468:1489	further investigations of the sialylation potentials	1468:1519	These results indicate that B. mori genome encodes an α2,6-sialyltransferase, but further investigations of the sialylation potentials are necessary.
26306633	7	42	theme	instar	1118:1123	arg1	larvae					1125:1130	B. mori fifth instar larvae	1104:1130	B. mori fifth instar larvae	1104:1130	N-Glycoprotein analysis of B. mori fifth instar larvae revealed that high-mannose-type structure was predominant and GlcNAc-linked and fucosylated structures were observed but endogenous galactosyl-, N-acetylgalactosaminyl- and sialyl-N-glycoproteins were undetectable under the standard analytical approach.
26306633	5	43	theme	Galβ1-R	866:872	arg1	terminus					854:861	the nonreducing terminus	838:861	the nonreducing terminus of Galβ1-R	838:872	Biochemical analysis using Sf9-expressed BmST revealed that BmST encoded α2,6-sialyltransferase and transferred N-acetylneuraminic acid (NeuAc) to the nonreducing terminus of Galβ1-R, but exhibited the highest activity toward GalNAcβ1,4-GlcNAc-R.
26306633	1	44	theme	important	127:135	arg1	modification					156:167	an important post-translational modification	124:167	an important post-translational modification in most secreted and membrane-bound proteins in eukaryotic cells	124:232	N-Glycosylation is an important post-translational modification in most secreted and membrane-bound proteins in eukaryotic cells.
26306633	1	44	theme	important	127:135	arg1	N-Glycosylation					105:119	N-Glycosylation	105:119	N-Glycosylation	105:119	N-Glycosylation is an important post-translational modification in most secreted and membrane-bound proteins in eukaryotic cells.
26306633	4	45	theme	development	651:661	arg1	stages					641:646	various stages	633:646	various stages of development	633:661	BmST was ubiquitously expressed in different organs and in various stages of development and localized at the Golgi.
26306633	0	46	dep	potentials	12:21	arg1	possesses					61:69	possesses	61:69	possesses an active α2,6-sialyltransferase	61:102	Sialylation potentials of the silkworm, Bombyx mori; B. mori possesses an active α2,6-sialyltransferase.
26306633	0	47	contain	possesses	61:69	arg1	mori					56:59	B. mori	53:59	B. mori	53:59	Sialylation potentials of the silkworm, Bombyx mori; B. mori possesses an active α2,6-sialyltransferase.
26306633	0	47	contain	possesses	61:69	arg2	α2,6-sialyltransferase					81:102	an active α2,6-sialyltransferase	71:102	an active α2,6-sialyltransferase	71:102	Sialylation potentials of the silkworm, Bombyx mori; B. mori possesses an active α2,6-sialyltransferase.
26306633	1	48	theme	post-translational	137:154	arg1	modification					156:167	an important post-translational modification	124:167	an important post-translational modification in most secreted and membrane-bound proteins in eukaryotic cells	124:232	N-Glycosylation is an important post-translational modification in most secreted and membrane-bound proteins in eukaryotic cells.
26306633	1	48	theme	post-translational	137:154	arg1	N-Glycosylation					105:119	N-Glycosylation	105:119	N-Glycosylation	105:119	N-Glycosylation is an important post-translational modification in most secreted and membrane-bound proteins in eukaryotic cells.
26306633	4	49	theme	various	633:639	arg1	stages					641:646	various stages	633:646	various stages of development	633:661	BmST was ubiquitously expressed in different organs and in various stages of development and localized at the Golgi.
26306633	5	50	theme	N-acetylneuraminic	803:820	arg1	NeuAc					828:832	NeuAc	828:832	NeuAc	828:832	Biochemical analysis using Sf9-expressed BmST revealed that BmST encoded α2,6-sialyltransferase and transferred N-acetylneuraminic acid (NeuAc) to the nonreducing terminus of Galβ1-R, but exhibited the highest activity toward GalNAcβ1,4-GlcNAc-R.
26306633	5	50	theme	N-acetylneuraminic	803:820	arg1	acid					822:825	N-acetylneuraminic acid	803:825	N-acetylneuraminic acid (NeuAc)	803:833	Biochemical analysis using Sf9-expressed BmST revealed that BmST encoded α2,6-sialyltransferase and transferred N-acetylneuraminic acid (NeuAc) to the nonreducing terminus of Galβ1-R, but exhibited the highest activity toward GalNAcβ1,4-GlcNAc-R.
26306633	2	51	theme	N-glycosylation	255:269	arg1	pathway					271:277	the insect N-glycosylation pathway	244:277	the insect N-glycosylation pathway	244:277	However, the insect N-glycosylation pathway and the potentials contributing to the N-glycan synthesis are still unclear because most of the studies on these subjects have focused on mammals and plants.
26306633	2	51	theme	N-glycosylation	255:269	arg1	unclear					347:353	unclear	347:353	unclear	347:353	However, the insect N-glycosylation pathway and the potentials contributing to the N-glycan synthesis are still unclear because most of the studies on these subjects have focused on mammals and plants.
24912815	2	0	theme	cTi	519:521	arg1	effect					509:514	the improvement effect	493:514	the improvement effect of cTi on diabetes-induced impaired implant osteointegration	493:575	We propose that the alterations in the Wnt/β-catenin pathway may play a role in mediating the improvement effect of cTi on diabetes-induced impaired implant osteointegration.
24912815	6	1	dep	cTi	1119:1121	arg1	treatment					1133:1141	treatment	1133:1141	treatment	1133:1141	Importantly, both cTi and Wnt3a treatment ameliorated osteoblastic dysfunction and apoptosis under diabetic condition.
24912815	1	2	theme	better	275:280	arg1	capacity					299:306	better osseointegration capacity	275:306	better osseointegration capacity	275:306	Composited Chitosan/Hydroxyapatite (CS/HA) material coated on titanium surface (cTi) is a promising approach to produce biomaterials with better osseointegration capacity, but its bio-performance under diabetic conditions and the mechanisms involved remain elusive.
24912815	0	3	theme	titanium	118:125	arg1	surfaces					127:134	porous titanium surfaces	111:134	porous titanium surfaces	111:134	The promotion of osteointegration under diabetic conditions using chitosan/hydroxyapatite composite coating on porous titanium surfaces.
24912815	9	4	theme	diabetic	1731:1738	arg1	patients					1740:1747	diabetic patients	1731:1747	diabetic patients	1731:1747	Our study explicitly demonstrates that CS/HA composite material improves diabetes-induced impaired osteointegration of Ti via the reactivation of Wnt/β-catenin pathway and provides a target point for biomaterial modification to attain better clinical performance in diabetic patients.
24912815	9	5	theme	biomaterial	1665:1675	arg1	modification					1677:1688	biomaterial modification	1665:1688	biomaterial modification	1665:1688	Our study explicitly demonstrates that CS/HA composite material improves diabetes-induced impaired osteointegration of Ti via the reactivation of Wnt/β-catenin pathway and provides a target point for biomaterial modification to attain better clinical performance in diabetic patients.
24912815	8	6	theme	aforementioned	1366:1379	arg1	effects					1391:1397	the aforementioned promotive effects	1362:1397	the aforementioned promotive effects afforded by cTi	1362:1413	Moreover, the aforementioned promotive effects afforded by cTi were abolished by blocking Wnt pathway with Dkk1.
24912815	9	7	theme	target	1648:1653	arg1	point					1655:1659	a target point	1646:1659	a target point for biomaterial modification	1646:1688	Our study explicitly demonstrates that CS/HA composite material improves diabetes-induced impaired osteointegration of Ti via the reactivation of Wnt/β-catenin pathway and provides a target point for biomaterial modification to attain better clinical performance in diabetic patients.
24912815	1	8	theme	osseointegration	282:297	arg1	capacity					299:306	better osseointegration capacity	275:306	better osseointegration capacity	275:306	Composited Chitosan/Hydroxyapatite (CS/HA) material coated on titanium surface (cTi) is a promising approach to produce biomaterials with better osseointegration capacity, but its bio-performance under diabetic conditions and the mechanisms involved remain elusive.
24912815	0	9	theme	porous	111:116	arg1	surfaces					127:134	porous titanium surfaces	111:134	porous titanium surfaces	111:134	The promotion of osteointegration under diabetic conditions using chitosan/hydroxyapatite composite coating on porous titanium surfaces.
24912815	6	10	theme	osteoblastic	1155:1166	arg1	dysfunction					1168:1178	osteoblastic dysfunction	1155:1178	osteoblastic dysfunction	1155:1178	Importantly, both cTi and Wnt3a treatment ameliorated osteoblastic dysfunction and apoptosis under diabetic condition.
24912815	9	11	theme	composite	1510:1518	arg1	material					1520:1527	CS/HA composite material	1504:1527	CS/HA composite material	1504:1527	Our study explicitly demonstrates that CS/HA composite material improves diabetes-induced impaired osteointegration of Ti via the reactivation of Wnt/β-catenin pathway and provides a target point for biomaterial modification to attain better clinical performance in diabetic patients.
24912815	2	12	theme	implant	552:558	arg1	osteointegration					560:575	diabetes-induced impaired implant osteointegration	526:575	diabetes-induced impaired implant osteointegration	526:575	We propose that the alterations in the Wnt/β-catenin pathway may play a role in mediating the improvement effect of cTi on diabetes-induced impaired implant osteointegration.
24912815	3	13	theme	specific	725:732	arg1	agonist					738:744	a specific Wnt agonist	723:744	a specific Wnt agonist	723:744	To confirm the hypothesis, primary rat osteoblasts incubated on Ti and cTi were subjected to normal serum (NS), diabetic serum (DS), DS + Wnt3a (a specific Wnt agonist) and DS + Dkk1 (a specific Wnt antagonist) treatment.
24912815	3	13	theme	specific	725:732	arg1	DS + Wnt3a					711:720	DS + Wnt3a	711:720	DS + Wnt3a (a specific Wnt agonist)	711:745	To confirm the hypothesis, primary rat osteoblasts incubated on Ti and cTi were subjected to normal serum (NS), diabetic serum (DS), DS + Wnt3a (a specific Wnt agonist) and DS + Dkk1 (a specific Wnt antagonist) treatment.
24912815	5	14	theme	osteoblast	949:958	arg1	function					960:967	osteoblast function	949:967	osteoblast function evidenced by impaired cell adhesion and morphology	949:1018	Results showed that diabetes depressed osteoblast function evidenced by impaired cell adhesion and morphology, decreased cell proliferation and ALP activity, and higher apoptotic rate on Ti.
24912815	1	15	theme	titanium	199:206	arg1	cTi					217:219	cTi	217:219	cTi	217:219	Composited Chitosan/Hydroxyapatite (CS/HA) material coated on titanium surface (cTi) is a promising approach to produce biomaterials with better osseointegration capacity, but its bio-performance under diabetic conditions and the mechanisms involved remain elusive.
24912815	1	15	theme	titanium	199:206	arg1	surface					208:214	titanium surface	199:214	titanium surface (cTi)	199:220	Composited Chitosan/Hydroxyapatite (CS/HA) material coated on titanium surface (cTi) is a promising approach to produce biomaterials with better osseointegration capacity, but its bio-performance under diabetic conditions and the mechanisms involved remain elusive.
24912815	2	16	theme	impaired	543:550	arg1	osteointegration					560:575	diabetes-induced impaired implant osteointegration	526:575	diabetes-induced impaired implant osteointegration	526:575	We propose that the alterations in the Wnt/β-catenin pathway may play a role in mediating the improvement effect of cTi on diabetes-induced impaired implant osteointegration.
24912815	2	17	from	effect	509:514	arg1	osteointegration					560:575	diabetes-induced impaired implant osteointegration	526:575	diabetes-induced impaired implant osteointegration	526:575	We propose that the alterations in the Wnt/β-catenin pathway may play a role in mediating the improvement effect of cTi on diabetes-induced impaired implant osteointegration.
24912815	8	18	theme	promotive	1381:1389	arg1	effects					1391:1397	the aforementioned promotive effects	1362:1397	the aforementioned promotive effects afforded by cTi	1362:1413	Moreover, the aforementioned promotive effects afforded by cTi were abolished by blocking Wnt pathway with Dkk1.
24912815	5	19	from	rate	1089:1092	arg1	Ti					1097:1098	Ti	1097:1098	Ti	1097:1098	Results showed that diabetes depressed osteoblast function evidenced by impaired cell adhesion and morphology, decreased cell proliferation and ALP activity, and higher apoptotic rate on Ti.
24912815	7	20	theme	histological	1308:1319	arg1	examinations					1321:1332	histological examinations	1308:1332	histological examinations	1308:1332	Implantation with cTi significantly improved osteointegration evidenced by Micro-CT and histological examinations compared with Ti.
24912815	0	21	theme	composite	90:98	arg1	coating					100:106	chitosan/hydroxyapatite composite coating	66:106	chitosan/hydroxyapatite composite coating on porous titanium surfaces	66:134	The promotion of osteointegration under diabetic conditions using chitosan/hydroxyapatite composite coating on porous titanium surfaces.
24912815	3	22	theme	rat	613:615	arg1	osteoblasts					617:627	primary rat osteoblasts	605:627	primary rat osteoblasts incubated on Ti and cTi	605:651	To confirm the hypothesis, primary rat osteoblasts incubated on Ti and cTi were subjected to normal serum (NS), diabetic serum (DS), DS + Wnt3a (a specific Wnt agonist) and DS + Dkk1 (a specific Wnt antagonist) treatment.
24912815	5	23	theme	apoptotic	1079:1087	arg1	rate					1089:1092	apoptotic rate	1079:1092	apoptotic rate	1079:1092	Results showed that diabetes depressed osteoblast function evidenced by impaired cell adhesion and morphology, decreased cell proliferation and ALP activity, and higher apoptotic rate on Ti.
24912815	4	24	theme	diabetic	831:838	arg1	sheep					840:844	diabetic sheep	831:844	diabetic sheep implanted with Ti or cTi into the bone defect on crista iliaca	831:907	In vivo study was performed on diabetic sheep implanted with Ti or cTi into the bone defect on crista iliaca.
24912815	0	25	theme	osteointegration	17:32	arg1	promotion					4:12	The promotion	0:12	The promotion of osteointegration under diabetic conditions using chitosan/hydroxyapatite composite coating on porous titanium surfaces.	0:135	The promotion of osteointegration under diabetic conditions using chitosan/hydroxyapatite composite coating on porous titanium surfaces.
24912815	7	26	with	Implantation	1220:1231	arg1	cTi					1238:1240	cTi	1238:1240	cTi	1238:1240	Implantation with cTi significantly improved osteointegration evidenced by Micro-CT and histological examinations compared with Ti.
24912815	3	27	theme	Wnt	734:736	arg1	agonist					738:744	a specific Wnt agonist	723:744	a specific Wnt agonist	723:744	To confirm the hypothesis, primary rat osteoblasts incubated on Ti and cTi were subjected to normal serum (NS), diabetic serum (DS), DS + Wnt3a (a specific Wnt agonist) and DS + Dkk1 (a specific Wnt antagonist) treatment.
24912815	3	27	theme	Wnt	734:736	arg1	DS + Wnt3a					711:720	DS + Wnt3a	711:720	DS + Wnt3a (a specific Wnt agonist)	711:745	To confirm the hypothesis, primary rat osteoblasts incubated on Ti and cTi were subjected to normal serum (NS), diabetic serum (DS), DS + Wnt3a (a specific Wnt agonist) and DS + Dkk1 (a specific Wnt antagonist) treatment.
24912815	9	28	theme	Wnt/β-catenin	1611:1623	arg1	pathway					1625:1631	Wnt/β-catenin pathway	1611:1631	Wnt/β-catenin pathway	1611:1631	Our study explicitly demonstrates that CS/HA composite material improves diabetes-induced impaired osteointegration of Ti via the reactivation of Wnt/β-catenin pathway and provides a target point for biomaterial modification to attain better clinical performance in diabetic patients.
24912815	5	29	theme	ALP	1054:1056	arg1	activity					1058:1065	ALP activity	1054:1065	ALP activity	1054:1065	Results showed that diabetes depressed osteoblast function evidenced by impaired cell adhesion and morphology, decreased cell proliferation and ALP activity, and higher apoptotic rate on Ti.
24912815	3	30	theme	primary	605:611	arg1	osteoblasts					617:627	primary rat osteoblasts	605:627	primary rat osteoblasts incubated on Ti and cTi	605:651	To confirm the hypothesis, primary rat osteoblasts incubated on Ti and cTi were subjected to normal serum (NS), diabetic serum (DS), DS + Wnt3a (a specific Wnt agonist) and DS + Dkk1 (a specific Wnt antagonist) treatment.
24912815	3	31	theme	normal	671:676	arg1	NS					685:686	NS	685:686	NS	685:686	To confirm the hypothesis, primary rat osteoblasts incubated on Ti and cTi were subjected to normal serum (NS), diabetic serum (DS), DS + Wnt3a (a specific Wnt agonist) and DS + Dkk1 (a specific Wnt antagonist) treatment.
24912815	3	31	theme	normal	671:676	arg1	serum					678:682	normal serum	671:682	normal serum (NS)	671:687	To confirm the hypothesis, primary rat osteoblasts incubated on Ti and cTi were subjected to normal serum (NS), diabetic serum (DS), DS + Wnt3a (a specific Wnt agonist) and DS + Dkk1 (a specific Wnt antagonist) treatment.
24912815	9	32	theme	CS/HA	1504:1508	arg1	material					1520:1527	CS/HA composite material	1504:1527	CS/HA composite material	1504:1527	Our study explicitly demonstrates that CS/HA composite material improves diabetes-induced impaired osteointegration of Ti via the reactivation of Wnt/β-catenin pathway and provides a target point for biomaterial modification to attain better clinical performance in diabetic patients.
24912815	0	33	theme	diabetic	40:47	arg1	conditions					49:58	diabetic conditions	40:58	diabetic conditions using chitosan/hydroxyapatite composite coating on porous titanium surfaces	40:134	The promotion of osteointegration under diabetic conditions using chitosan/hydroxyapatite composite coating on porous titanium surfaces.
24912815	9	34	theme	diabetes-induced	1538:1553	arg1	osteointegration					1564:1579	diabetes-induced impaired osteointegration	1538:1579	diabetes-induced impaired osteointegration of Ti	1538:1585	Our study explicitly demonstrates that CS/HA composite material improves diabetes-induced impaired osteointegration of Ti via the reactivation of Wnt/β-catenin pathway and provides a target point for biomaterial modification to attain better clinical performance in diabetic patients.
24912815	0	35	from	coating	100:106	arg1	surfaces					127:134	porous titanium surfaces	111:134	porous titanium surfaces	111:134	The promotion of osteointegration under diabetic conditions using chitosan/hydroxyapatite composite coating on porous titanium surfaces.
24912815	4	36	theme	In vivo	800:806	arg1	study					808:812	In vivo study	800:812	In vivo study	800:812	In vivo study was performed on diabetic sheep implanted with Ti or cTi into the bone defect on crista iliaca.
24912815	2	37	theme	Wnt/β-catenin	442:454	arg1	pathway					456:462	the Wnt/β-catenin pathway	438:462	the Wnt/β-catenin pathway	438:462	We propose that the alterations in the Wnt/β-catenin pathway may play a role in mediating the improvement effect of cTi on diabetes-induced impaired implant osteointegration.
24912815	2	38	theme	improvement	497:507	arg1	effect					509:514	the improvement effect	493:514	the improvement effect of cTi on diabetes-induced impaired implant osteointegration	493:575	We propose that the alterations in the Wnt/β-catenin pathway may play a role in mediating the improvement effect of cTi on diabetes-induced impaired implant osteointegration.
24912815	5	39	dep	rate	1089:1092	arg1	higher					1072:1077	higher	1072:1077	higher	1072:1077	Results showed that diabetes depressed osteoblast function evidenced by impaired cell adhesion and morphology, decreased cell proliferation and ALP activity, and higher apoptotic rate on Ti.
24912815	4	40	theme	crista	895:900	arg1	iliaca					902:907	crista iliaca	895:907	crista iliaca	895:907	In vivo study was performed on diabetic sheep implanted with Ti or cTi into the bone defect on crista iliaca.
24912815	1	41	dep	approach	237:244	arg1	produce					249:255	produce	249:255	to produce biomaterials with better osseointegration capacity, but its bio-performance under diabetic conditions	246:357	Composited Chitosan/Hydroxyapatite (CS/HA) material coated on titanium surface (cTi) is a promising approach to produce biomaterials with better osseointegration capacity, but its bio-performance under diabetic conditions and the mechanisms involved remain elusive.
24912815	1	42	theme	promising	227:235	arg1	material					180:187	Composited Chitosan/Hydroxyapatite (CS/HA) material	137:187	Composited Chitosan/Hydroxyapatite (CS/HA) material coated on titanium surface (cTi)	137:220	Composited Chitosan/Hydroxyapatite (CS/HA) material coated on titanium surface (cTi) is a promising approach to produce biomaterials with better osseointegration capacity, but its bio-performance under diabetic conditions and the mechanisms involved remain elusive.
24912815	1	42	theme	promising	227:235	arg1	approach					237:244	a promising approach	225:244	a promising approach to produce biomaterials with better osseointegration capacity, but its bio-performance under diabetic conditions	225:357	Composited Chitosan/Hydroxyapatite (CS/HA) material coated on titanium surface (cTi) is a promising approach to produce biomaterials with better osseointegration capacity, but its bio-performance under diabetic conditions and the mechanisms involved remain elusive.
24912815	1	43	theme	diabetic	339:346	arg1	conditions					348:357	diabetic conditions	339:357	diabetic conditions	339:357	Composited Chitosan/Hydroxyapatite (CS/HA) material coated on titanium surface (cTi) is a promising approach to produce biomaterials with better osseointegration capacity, but its bio-performance under diabetic conditions and the mechanisms involved remain elusive.
24912815	4	44	from	defect	885:890	arg1	iliaca					902:907	crista iliaca	895:907	crista iliaca	895:907	In vivo study was performed on diabetic sheep implanted with Ti or cTi into the bone defect on crista iliaca.
24912815	8	45	theme	Wnt	1442:1444	arg1	pathway					1446:1452	Wnt pathway	1442:1452	Wnt pathway	1442:1452	Moreover, the aforementioned promotive effects afforded by cTi were abolished by blocking Wnt pathway with Dkk1.
24912815	1	46	theme	Composited	137:146	arg1	material					180:187	Composited Chitosan/Hydroxyapatite (CS/HA) material	137:187	Composited Chitosan/Hydroxyapatite (CS/HA) material coated on titanium surface (cTi)	137:220	Composited Chitosan/Hydroxyapatite (CS/HA) material coated on titanium surface (cTi) is a promising approach to produce biomaterials with better osseointegration capacity, but its bio-performance under diabetic conditions and the mechanisms involved remain elusive.
24912815	1	46	theme	Composited	137:146	arg1	approach					237:244	a promising approach	225:244	a promising approach to produce biomaterials with better osseointegration capacity, but its bio-performance under diabetic conditions	225:357	Composited Chitosan/Hydroxyapatite (CS/HA) material coated on titanium surface (cTi) is a promising approach to produce biomaterials with better osseointegration capacity, but its bio-performance under diabetic conditions and the mechanisms involved remain elusive.
24912815	3	47	theme	diabetic	690:697	arg1	DS					706:707	DS	706:707	DS	706:707	To confirm the hypothesis, primary rat osteoblasts incubated on Ti and cTi were subjected to normal serum (NS), diabetic serum (DS), DS + Wnt3a (a specific Wnt agonist) and DS + Dkk1 (a specific Wnt antagonist) treatment.
24912815	3	47	theme	diabetic	690:697	arg1	serum					699:703	diabetic serum	690:703	diabetic serum (DS)	690:708	To confirm the hypothesis, primary rat osteoblasts incubated on Ti and cTi were subjected to normal serum (NS), diabetic serum (DS), DS + Wnt3a (a specific Wnt agonist) and DS + Dkk1 (a specific Wnt antagonist) treatment.
24912815	9	48	theme	better	1700:1705	arg1	performance					1716:1726	better clinical performance	1700:1726	better clinical performance	1700:1726	Our study explicitly demonstrates that CS/HA composite material improves diabetes-induced impaired osteointegration of Ti via the reactivation of Wnt/β-catenin pathway and provides a target point for biomaterial modification to attain better clinical performance in diabetic patients.
24912815	1	49	theme	Chitosan/Hydroxyapatite	148:170	arg1	material					180:187	Composited Chitosan/Hydroxyapatite (CS/HA) material	137:187	Composited Chitosan/Hydroxyapatite (CS/HA) material coated on titanium surface (cTi)	137:220	Composited Chitosan/Hydroxyapatite (CS/HA) material coated on titanium surface (cTi) is a promising approach to produce biomaterials with better osseointegration capacity, but its bio-performance under diabetic conditions and the mechanisms involved remain elusive.
24912815	1	49	theme	Chitosan/Hydroxyapatite	148:170	arg1	approach					237:244	a promising approach	225:244	a promising approach to produce biomaterials with better osseointegration capacity, but its bio-performance under diabetic conditions	225:357	Composited Chitosan/Hydroxyapatite (CS/HA) material coated on titanium surface (cTi) is a promising approach to produce biomaterials with better osseointegration capacity, but its bio-performance under diabetic conditions and the mechanisms involved remain elusive.
24912815	9	50	theme	clinical	1707:1714	arg1	performance					1716:1726	better clinical performance	1700:1726	better clinical performance	1700:1726	Our study explicitly demonstrates that CS/HA composite material improves diabetes-induced impaired osteointegration of Ti via the reactivation of Wnt/β-catenin pathway and provides a target point for biomaterial modification to attain better clinical performance in diabetic patients.
24912815	4	51	theme	bone	880:883	arg1	defect					885:890	the bone defect	876:890	the bone defect on crista iliaca	876:907	In vivo study was performed on diabetic sheep implanted with Ti or cTi into the bone defect on crista iliaca.
24912815	5	52	theme	cell	1031:1034	arg1	proliferation					1036:1048	cell proliferation	1031:1048	cell proliferation	1031:1048	Results showed that diabetes depressed osteoblast function evidenced by impaired cell adhesion and morphology, decreased cell proliferation and ALP activity, and higher apoptotic rate on Ti.
24912815	6	53	theme	diabetic	1200:1207	arg1	condition					1209:1217	diabetic condition	1200:1217	diabetic condition	1200:1217	Importantly, both cTi and Wnt3a treatment ameliorated osteoblastic dysfunction and apoptosis under diabetic condition.
24912815	2	54	from	alterations	423:433	arg1	pathway					456:462	the Wnt/β-catenin pathway	438:462	the Wnt/β-catenin pathway	438:462	We propose that the alterations in the Wnt/β-catenin pathway may play a role in mediating the improvement effect of cTi on diabetes-induced impaired implant osteointegration.
24912815	9	55	theme	impaired	1555:1562	arg1	osteointegration					1564:1579	diabetes-induced impaired osteointegration	1538:1579	diabetes-induced impaired osteointegration of Ti	1538:1585	Our study explicitly demonstrates that CS/HA composite material improves diabetes-induced impaired osteointegration of Ti via the reactivation of Wnt/β-catenin pathway and provides a target point for biomaterial modification to attain better clinical performance in diabetic patients.
24912815	9	56	theme	pathway	1625:1631	arg1	reactivation					1595:1606	the reactivation	1591:1606	the reactivation of Wnt/β-catenin pathway	1591:1631	Our study explicitly demonstrates that CS/HA composite material improves diabetes-induced impaired osteointegration of Ti via the reactivation of Wnt/β-catenin pathway and provides a target point for biomaterial modification to attain better clinical performance in diabetic patients.
24912815	1	57	theme	CS/HA	173:177	arg1	material					180:187	Composited Chitosan/Hydroxyapatite (CS/HA) material	137:187	Composited Chitosan/Hydroxyapatite (CS/HA) material coated on titanium surface (cTi)	137:220	Composited Chitosan/Hydroxyapatite (CS/HA) material coated on titanium surface (cTi) is a promising approach to produce biomaterials with better osseointegration capacity, but its bio-performance under diabetic conditions and the mechanisms involved remain elusive.
24912815	1	57	theme	CS/HA	173:177	arg1	approach					237:244	a promising approach	225:244	a promising approach to produce biomaterials with better osseointegration capacity, but its bio-performance under diabetic conditions	225:357	Composited Chitosan/Hydroxyapatite (CS/HA) material coated on titanium surface (cTi) is a promising approach to produce biomaterials with better osseointegration capacity, but its bio-performance under diabetic conditions and the mechanisms involved remain elusive.
24912815	3	58	dep	serum	678:682	arg1	treatment					789:797	treatment	789:797	treatment	789:797	To confirm the hypothesis, primary rat osteoblasts incubated on Ti and cTi were subjected to normal serum (NS), diabetic serum (DS), DS + Wnt3a (a specific Wnt agonist) and DS + Dkk1 (a specific Wnt antagonist) treatment.
24912815	2	59	theme	diabetes-induced	526:541	arg1	osteointegration					560:575	diabetes-induced impaired implant osteointegration	526:575	diabetes-induced impaired implant osteointegration	526:575	We propose that the alterations in the Wnt/β-catenin pathway may play a role in mediating the improvement effect of cTi on diabetes-induced impaired implant osteointegration.
24912815	5	60	theme	impaired	982:989	arg1	adhesion					996:1003	impaired cell adhesion	982:1003	impaired cell adhesion	982:1003	Results showed that diabetes depressed osteoblast function evidenced by impaired cell adhesion and morphology, decreased cell proliferation and ALP activity, and higher apoptotic rate on Ti.
24912815	1	61	with	biomaterials	257:268	arg1	bio-performance					317:331	its bio-performance	313:331	its bio-performance under diabetic conditions	313:357	Composited Chitosan/Hydroxyapatite (CS/HA) material coated on titanium surface (cTi) is a promising approach to produce biomaterials with better osseointegration capacity, but its bio-performance under diabetic conditions and the mechanisms involved remain elusive.
24912815	1	61	with	biomaterials	257:268	arg1	capacity					299:306	better osseointegration capacity	275:306	better osseointegration capacity	275:306	Composited Chitosan/Hydroxyapatite (CS/HA) material coated on titanium surface (cTi) is a promising approach to produce biomaterials with better osseointegration capacity, but its bio-performance under diabetic conditions and the mechanisms involved remain elusive.
24912815	3	62	theme	Wnt	773:775	arg1	antagonist					777:786	a specific Wnt antagonist	762:786	a specific Wnt antagonist	762:786	To confirm the hypothesis, primary rat osteoblasts incubated on Ti and cTi were subjected to normal serum (NS), diabetic serum (DS), DS + Wnt3a (a specific Wnt agonist) and DS + Dkk1 (a specific Wnt antagonist) treatment.
24912815	3	62	theme	Wnt	773:775	arg1	DS + Dkk1					751:759	DS + Dkk1	751:759	DS + Dkk1 (a specific Wnt antagonist)	751:787	To confirm the hypothesis, primary rat osteoblasts incubated on Ti and cTi were subjected to normal serum (NS), diabetic serum (DS), DS + Wnt3a (a specific Wnt agonist) and DS + Dkk1 (a specific Wnt antagonist) treatment.
24912815	0	63	theme	chitosan/hydroxyapatite	66:88	arg1	coating					100:106	chitosan/hydroxyapatite composite coating	66:106	chitosan/hydroxyapatite composite coating on porous titanium surfaces	66:134	The promotion of osteointegration under diabetic conditions using chitosan/hydroxyapatite composite coating on porous titanium surfaces.
24912815	5	64	theme	cell	991:994	arg1	adhesion					996:1003	impaired cell adhesion	982:1003	impaired cell adhesion	982:1003	Results showed that diabetes depressed osteoblast function evidenced by impaired cell adhesion and morphology, decreased cell proliferation and ALP activity, and higher apoptotic rate on Ti.
24912815	9	65	theme	Ti	1584:1585	arg1	osteointegration					1564:1579	diabetes-induced impaired osteointegration	1538:1579	diabetes-induced impaired osteointegration of Ti	1538:1585	Our study explicitly demonstrates that CS/HA composite material improves diabetes-induced impaired osteointegration of Ti via the reactivation of Wnt/β-catenin pathway and provides a target point for biomaterial modification to attain better clinical performance in diabetic patients.
24912815	3	66	theme	specific	764:771	arg1	antagonist					777:786	a specific Wnt antagonist	762:786	a specific Wnt antagonist	762:786	To confirm the hypothesis, primary rat osteoblasts incubated on Ti and cTi were subjected to normal serum (NS), diabetic serum (DS), DS + Wnt3a (a specific Wnt agonist) and DS + Dkk1 (a specific Wnt antagonist) treatment.
24912815	3	66	theme	specific	764:771	arg1	DS + Dkk1					751:759	DS + Dkk1	751:759	DS + Dkk1 (a specific Wnt antagonist)	751:787	To confirm the hypothesis, primary rat osteoblasts incubated on Ti and cTi were subjected to normal serum (NS), diabetic serum (DS), DS + Wnt3a (a specific Wnt agonist) and DS + Dkk1 (a specific Wnt antagonist) treatment.
26868559	8	0	contain	have	1108:1111	arg1	flours					1059:1064	these grape pomace flours	1040:1064	these grape pomace flours	1040:1064	These findings indicate that these grape pomace flours are rich in antioxidant dietary fibre and have a potential use as food ingredients.
26868559	8	0	contain	have	1108:1111	arg2	use					1125:1127	a potential use	1113:1127	a potential use as food ingredients	1113:1147	These findings indicate that these grape pomace flours are rich in antioxidant dietary fibre and have a potential use as food ingredients.
26868559	1	1	theme	factorial	132:140	arg1	experiment					142:151	a factorial experiment	130:151	a factorial experiment	130:151	Brazilian grape pomace was extracted in hot water, and a factorial experiment was used to evaluate polysaccharide recovery.
26868559	5	2	theme	pectic-	712:718	arg1	polysaccharides					738:752	pectic- and glucose-based polysaccharides	712:752	pectic- and glucose-based polysaccharides	712:752	(13)C NMR and HSQC spectra confirmed the presence of pectic- and glucose-based polysaccharides in the extracts.
26868559	8	3	theme	food	1132:1135	arg1	ingredients					1137:1147	food ingredients	1132:1147	food ingredients	1132:1147	These findings indicate that these grape pomace flours are rich in antioxidant dietary fibre and have a potential use as food ingredients.
26868559	4	4	theme	monosaccharide	489:502	arg1	composition					504:514	The monosaccharide composition	485:514	The monosaccharide composition of extracts obtained from flours	485:547	The monosaccharide composition of extracts obtained from flours were, on average, Rha:Ara:Xyl:Man:Gal:Glc:GalA in a 3:32:2:13:11:20:19 M ratio, with varying Glc:GalA ratios.
26868559	4	5	theme	varying	634:640	arg1	ratios					651:656	varying Glc:GalA ratios	634:656	varying Glc:GalA ratios	634:656	The monosaccharide composition of extracts obtained from flours were, on average, Rha:Ara:Xyl:Man:Gal:Glc:GalA in a 3:32:2:13:11:20:19 M ratio, with varying Glc:GalA ratios.
26868559	7	6	theme	radical	936:942	arg1	capacity					955:962	ABTS radical scavenging capacity	931:962	ABTS radical scavenging capacity (from 8.00 to 46.60 mMol Trolox/100 g pomace)	931:1008	The extracts also had ABTS radical scavenging capacity (from 8.00 to 46.60 mMol Trolox/100 g pomace).
26868559	7	6	theme	radical	936:942	arg1	pomace					1002:1007	from 8.00 to 46.60 mMol Trolox/100 g pomace	965:1007	from 8.00 to 46.60 mMol Trolox/100 g pomace	965:1007	The extracts also had ABTS radical scavenging capacity (from 8.00 to 46.60 mMol Trolox/100 g pomace).
26868559	2	7	theme	solvent	270:276	arg1	ratio					278:282	solute:solvent ratio	263:282	solute:solvent ratio	263:282	The dependent variables were the temperature, particle size and solute:solvent ratio.
26868559	4	8	theme	extracts	519:526	arg1	composition					504:514	The monosaccharide composition	485:514	The monosaccharide composition of extracts obtained from flours	485:547	The monosaccharide composition of extracts obtained from flours were, on average, Rha:Ara:Xyl:Man:Gal:Glc:GalA in a 3:32:2:13:11:20:19 M ratio, with varying Glc:GalA ratios.
26868559	7	9	theme	scavenging	944:953	arg1	capacity					955:962	ABTS radical scavenging capacity	931:962	ABTS radical scavenging capacity (from 8.00 to 46.60 mMol Trolox/100 g pomace)	931:1008	The extracts also had ABTS radical scavenging capacity (from 8.00 to 46.60 mMol Trolox/100 g pomace).
26868559	7	9	theme	scavenging	944:953	arg1	pomace					1002:1007	from 8.00 to 46.60 mMol Trolox/100 g pomace	965:1007	from 8.00 to 46.60 mMol Trolox/100 g pomace	965:1007	The extracts also had ABTS radical scavenging capacity (from 8.00 to 46.60 mMol Trolox/100 g pomace).
26868559	5	10	from	presence	700:707	arg1	extracts					761:768	the extracts	757:768	the extracts	757:768	(13)C NMR and HSQC spectra confirmed the presence of pectic- and glucose-based polysaccharides in the extracts.
26868559	4	11	theme	M	620:620	arg1	ratio					622:626	a 3:32:2:13:11:20:19 M ratio	599:626	a 3:32:2:13:11:20:19 M ratio	599:626	The monosaccharide composition of extracts obtained from flours were, on average, Rha:Ara:Xyl:Man:Gal:Glc:GalA in a 3:32:2:13:11:20:19 M ratio, with varying Glc:GalA ratios.
26868559	5	12	attach	presence	700:707	arg2	polysaccharides					738:752	pectic- and glucose-based polysaccharides	712:752	pectic- and glucose-based polysaccharides	712:752	(13)C NMR and HSQC spectra confirmed the presence of pectic- and glucose-based polysaccharides in the extracts.
26868559	5	12	attach	presence	700:707	arg1	extracts					761:768	the extracts	757:768	the extracts	757:768	(13)C NMR and HSQC spectra confirmed the presence of pectic- and glucose-based polysaccharides in the extracts.
26868559	7	13	theme	Trolox/100	989:998	arg1	capacity					955:962	ABTS radical scavenging capacity	931:962	ABTS radical scavenging capacity (from 8.00 to 46.60 mMol Trolox/100 g pomace)	931:1008	The extracts also had ABTS radical scavenging capacity (from 8.00 to 46.60 mMol Trolox/100 g pomace).
26868559	7	13	theme	Trolox/100	989:998	arg1	pomace					1002:1007	from 8.00 to 46.60 mMol Trolox/100 g pomace	965:1007	from 8.00 to 46.60 mMol Trolox/100 g pomace	965:1007	The extracts also had ABTS radical scavenging capacity (from 8.00 to 46.60 mMol Trolox/100 g pomace).
26868559	2	14	theme	solute	263:268	arg1	ratio					278:282	solute:solvent ratio	263:282	solute:solvent ratio	263:282	The dependent variables were the temperature, particle size and solute:solvent ratio.
26868559	5	15	theme	HSQC	673:676	arg1	spectra					678:684	HSQC spectra	673:684	HSQC spectra	673:684	(13)C NMR and HSQC spectra confirmed the presence of pectic- and glucose-based polysaccharides in the extracts.
26868559	3	16	theme	10	325:326	arg1	%					320:320	%	320:320	%	320:320	Polysaccharide yields varied from 3% to 10%, and the highest sugar content was observed when extraction was carried out at 100 °C from finely sized particles (⩽249 μm) in a 1:12 solute:solvent ratio.
26868559	7	17	theme	ABTS	931:934	arg1	capacity					955:962	ABTS radical scavenging capacity	931:962	ABTS radical scavenging capacity (from 8.00 to 46.60 mMol Trolox/100 g pomace)	931:1008	The extracts also had ABTS radical scavenging capacity (from 8.00 to 46.60 mMol Trolox/100 g pomace).
26868559	7	17	theme	ABTS	931:934	arg1	pomace					1002:1007	from 8.00 to 46.60 mMol Trolox/100 g pomace	965:1007	from 8.00 to 46.60 mMol Trolox/100 g pomace	965:1007	The extracts also had ABTS radical scavenging capacity (from 8.00 to 46.60 mMol Trolox/100 g pomace).
26868559	1	18	theme	Brazilian	75:83	arg1	pomace					91:96	Brazilian grape pomace	75:96	Brazilian grape pomace	75:96	Brazilian grape pomace was extracted in hot water, and a factorial experiment was used to evaluate polysaccharide recovery.
26868559	8	19	theme	pomace	1052:1057	arg1	flours					1059:1064	these grape pomace flours	1040:1064	these grape pomace flours	1040:1064	These findings indicate that these grape pomace flours are rich in antioxidant dietary fibre and have a potential use as food ingredients.
26868559	3	20	theme	highest	338:344	arg1	content					352:358	the highest sugar content	334:358	the highest sugar content	334:358	Polysaccharide yields varied from 3% to 10%, and the highest sugar content was observed when extraction was carried out at 100 °C from finely sized particles (⩽249 μm) in a 1:12 solute:solvent ratio.
26868559	1	21	theme	grape	85:89	arg1	pomace					91:96	Brazilian grape pomace	75:96	Brazilian grape pomace	75:96	Brazilian grape pomace was extracted in hot water, and a factorial experiment was used to evaluate polysaccharide recovery.
26868559	3	22	theme	sugar	346:350	arg1	content					352:358	the highest sugar content	334:358	the highest sugar content	334:358	Polysaccharide yields varied from 3% to 10%, and the highest sugar content was observed when extraction was carried out at 100 °C from finely sized particles (⩽249 μm) in a 1:12 solute:solvent ratio.
26868559	6	23	theme	pomace	807:812	arg1	extraction					814:823	pomace extraction	807:823	pomace extraction	807:823	Phenolic compounds were found after pomace extraction, and catechin, gallic acid and epicatechin were the principal compounds identified.
26868559	7	24	dep	46.60	978:982	arg1	to					975:976	to	975:976	to	975:976	The extracts also had ABTS radical scavenging capacity (from 8.00 to 46.60 mMol Trolox/100 g pomace).
26868559	3	25	from	particles	433:441	arg1	°C					412:413	100 °C	408:413	100 °C from finely sized particles (⩽249 μm)	408:451	Polysaccharide yields varied from 3% to 10%, and the highest sugar content was observed when extraction was carried out at 100 °C from finely sized particles (⩽249 μm) in a 1:12 solute:solvent ratio.
26868559	7	26	theme	mMol	984:987	arg1	capacity					955:962	ABTS radical scavenging capacity	931:962	ABTS radical scavenging capacity (from 8.00 to 46.60 mMol Trolox/100 g pomace)	931:1008	The extracts also had ABTS radical scavenging capacity (from 8.00 to 46.60 mMol Trolox/100 g pomace).
26868559	7	26	theme	mMol	984:987	arg1	pomace					1002:1007	from 8.00 to 46.60 mMol Trolox/100 g pomace	965:1007	from 8.00 to 46.60 mMol Trolox/100 g pomace	965:1007	The extracts also had ABTS radical scavenging capacity (from 8.00 to 46.60 mMol Trolox/100 g pomace).
26868559	4	27	theme	GalA	646:649	arg1	ratios					651:656	varying Glc:GalA ratios	634:656	varying Glc:GalA ratios	634:656	The monosaccharide composition of extracts obtained from flours were, on average, Rha:Ara:Xyl:Man:Gal:Glc:GalA in a 3:32:2:13:11:20:19 M ratio, with varying Glc:GalA ratios.
26868559	3	28	dep	varied	307:312	arg1	ratio					478:482	solvent ratio	470:482	solvent ratio	470:482	Polysaccharide yields varied from 3% to 10%, and the highest sugar content was observed when extraction was carried out at 100 °C from finely sized particles (⩽249 μm) in a 1:12 solute:solvent ratio.
26868559	4	29	from	GalA	591:594	arg1	ratio					622:626	a 3:32:2:13:11:20:19 M ratio	599:626	a 3:32:2:13:11:20:19 M ratio	599:626	The monosaccharide composition of extracts obtained from flours were, on average, Rha:Ara:Xyl:Man:Gal:Glc:GalA in a 3:32:2:13:11:20:19 M ratio, with varying Glc:GalA ratios.
26868559	3	30	theme	solvent	470:476	arg1	ratio					478:482	solvent ratio	470:482	solvent ratio	470:482	Polysaccharide yields varied from 3% to 10%, and the highest sugar content was observed when extraction was carried out at 100 °C from finely sized particles (⩽249 μm) in a 1:12 solute:solvent ratio.
26868559	1	31	theme	polysaccharide	174:187	arg1	recovery					189:196	polysaccharide recovery	174:196	polysaccharide recovery	174:196	Brazilian grape pomace was extracted in hot water, and a factorial experiment was used to evaluate polysaccharide recovery.
26868559	0	32	theme	fibre	20:24	arg1	recovery					26:33	fibre recovery	20:33	fibre recovery from Brazilian Pinot noir grape pomace	20:72	Antioxidant dietary fibre recovery from Brazilian Pinot noir grape pomace.
26868559	5	33	theme	C	663:663	arg1	NMR					665:667	(13)C NMR	659:667	(13)C NMR	659:667	(13)C NMR and HSQC spectra confirmed the presence of pectic- and glucose-based polysaccharides in the extracts.
26868559	0	34	theme	Brazilian	40:48	arg1	pomace					67:72	Brazilian Pinot noir grape pomace	40:72	Brazilian Pinot noir grape pomace	40:72	Antioxidant dietary fibre recovery from Brazilian Pinot noir grape pomace.
26868559	5	35	theme	polysaccharides	738:752	arg1	presence					700:707	the presence	696:707	the presence of pectic- and glucose-based polysaccharides in the extracts	696:768	(13)C NMR and HSQC spectra confirmed the presence of pectic- and glucose-based polysaccharides in the extracts.
26868559	3	36	dep	%	320:320	arg1	to					322:323	to	322:323	to	322:323	Polysaccharide yields varied from 3% to 10%, and the highest sugar content was observed when extraction was carried out at 100 °C from finely sized particles (⩽249 μm) in a 1:12 solute:solvent ratio.
26868559	0	37	from	pomace	67:72	arg1	recovery					26:33	fibre recovery	20:33	fibre recovery from Brazilian Pinot noir grape pomace	20:72	Antioxidant dietary fibre recovery from Brazilian Pinot noir grape pomace.
26868559	8	38	from	rich	1070:1073	arg1	fibre					1098:1102	fibre	1098:1102	fibre	1098:1102	These findings indicate that these grape pomace flours are rich in antioxidant dietary fibre and have a potential use as food ingredients.
26868559	0	39	theme	noir	56:59	arg1	pomace					67:72	Brazilian Pinot noir grape pomace	40:72	Brazilian Pinot noir grape pomace	40:72	Antioxidant dietary fibre recovery from Brazilian Pinot noir grape pomace.
26868559	6	40	theme	Phenolic	771:778	arg1	compounds					780:788	Phenolic compounds	771:788	Phenolic compounds	771:788	Phenolic compounds were found after pomace extraction, and catechin, gallic acid and epicatechin were the principal compounds identified.
26868559	3	41	theme	Polysaccharide	285:298	arg1	yields					300:305	Polysaccharide yields	285:305	Polysaccharide yields	285:305	Polysaccharide yields varied from 3% to 10%, and the highest sugar content was observed when extraction was carried out at 100 °C from finely sized particles (⩽249 μm) in a 1:12 solute:solvent ratio.
26868559	5	42	theme	glucose-based	724:736	arg1	polysaccharides					738:752	pectic- and glucose-based polysaccharides	712:752	pectic- and glucose-based polysaccharides	712:752	(13)C NMR and HSQC spectra confirmed the presence of pectic- and glucose-based polysaccharides in the extracts.
26868559	0	43	theme	Pinot	50:54	arg1	pomace					67:72	Brazilian Pinot noir grape pomace	40:72	Brazilian Pinot noir grape pomace	40:72	Antioxidant dietary fibre recovery from Brazilian Pinot noir grape pomace.
26868559	2	44	theme	dependent	203:211	arg1	variables					213:221	The dependent variables	199:221	The dependent variables	199:221	The dependent variables were the temperature, particle size and solute:solvent ratio.
26868559	2	44	theme	dependent	203:211	arg1	temperature					232:242	the temperature	228:242	the temperature	228:242	The dependent variables were the temperature, particle size and solute:solvent ratio.
26868559	1	45	theme	hot	115:117	arg1	water					119:123	hot water	115:123	hot water	115:123	Brazilian grape pomace was extracted in hot water, and a factorial experiment was used to evaluate polysaccharide recovery.
26868559	4	46	with	average	558:564	arg1	ratios					651:656	varying Glc:GalA ratios	634:656	varying Glc:GalA ratios	634:656	The monosaccharide composition of extracts obtained from flours were, on average, Rha:Ara:Xyl:Man:Gal:Glc:GalA in a 3:32:2:13:11:20:19 M ratio, with varying Glc:GalA ratios.
26868559	4	47	theme	Glc	642:644	arg1	ratios					651:656	varying Glc:GalA ratios	634:656	varying Glc:GalA ratios	634:656	The monosaccharide composition of extracts obtained from flours were, on average, Rha:Ara:Xyl:Man:Gal:Glc:GalA in a 3:32:2:13:11:20:19 M ratio, with varying Glc:GalA ratios.
26868559	8	48	theme	potential	1115:1123	arg1	use					1125:1127	a potential use	1113:1127	a potential use as food ingredients	1113:1147	These findings indicate that these grape pomace flours are rich in antioxidant dietary fibre and have a potential use as food ingredients.
26868559	4	49	theme	3:32:2:13:11:20:19	601:618	arg1	ratio					622:626	a 3:32:2:13:11:20:19 M ratio	599:626	a 3:32:2:13:11:20:19 M ratio	599:626	The monosaccharide composition of extracts obtained from flours were, on average, Rha:Ara:Xyl:Man:Gal:Glc:GalA in a 3:32:2:13:11:20:19 M ratio, with varying Glc:GalA ratios.
26868559	3	50	theme	1:12	458:461	arg1	solute					463:468	a 1:12 solute	456:468	a 1:12 solute	456:468	Polysaccharide yields varied from 3% to 10%, and the highest sugar content was observed when extraction was carried out at 100 °C from finely sized particles (⩽249 μm) in a 1:12 solute:solvent ratio.
26868559	2	51	theme	particle	245:252	arg1	size					254:257	particle size	245:257	particle size	245:257	The dependent variables were the temperature, particle size and solute:solvent ratio.
26868559	8	52	from	fibre	1098:1102	arg1	rich					1070:1073	rich	1070:1073	rich	1070:1073	These findings indicate that these grape pomace flours are rich in antioxidant dietary fibre and have a potential use as food ingredients.
26868559	3	53	theme	sized	427:431	arg1	particles					433:441	finely sized particles	420:441	finely sized particles (⩽249 μm)	420:451	Polysaccharide yields varied from 3% to 10%, and the highest sugar content was observed when extraction was carried out at 100 °C from finely sized particles (⩽249 μm) in a 1:12 solute:solvent ratio.
26868559	3	53	theme	sized	427:431	arg1	μm					449:450	⩽249 μm	444:450	⩽249 μm	444:450	Polysaccharide yields varied from 3% to 10%, and the highest sugar content was observed when extraction was carried out at 100 °C from finely sized particles (⩽249 μm) in a 1:12 solute:solvent ratio.
26868559	8	54	theme	grape	1046:1050	arg1	flours					1059:1064	these grape pomace flours	1040:1064	these grape pomace flours	1040:1064	These findings indicate that these grape pomace flours are rich in antioxidant dietary fibre and have a potential use as food ingredients.
26868559	3	55	theme	3	319:319	arg1	%					320:320	%	320:320	%	320:320	Polysaccharide yields varied from 3% to 10%, and the highest sugar content was observed when extraction was carried out at 100 °C from finely sized particles (⩽249 μm) in a 1:12 solute:solvent ratio.
26868559	1	56	used	used	157:160	arg2	experiment					142:151	a factorial experiment	130:151	a factorial experiment	130:151	Brazilian grape pomace was extracted in hot water, and a factorial experiment was used to evaluate polysaccharide recovery.
26868559	6	57	theme	principal	877:885	arg1	epicatechin					856:866	epicatechin	856:866	epicatechin	856:866	Phenolic compounds were found after pomace extraction, and catechin, gallic acid and epicatechin were the principal compounds identified.
26868559	6	57	theme	principal	877:885	arg1	catechin					830:837	catechin	830:837	catechin	830:837	Phenolic compounds were found after pomace extraction, and catechin, gallic acid and epicatechin were the principal compounds identified.
26868559	6	57	theme	principal	877:885	arg1	acid					847:850	gallic acid	840:850	gallic acid	840:850	Phenolic compounds were found after pomace extraction, and catechin, gallic acid and epicatechin were the principal compounds identified.
26868559	6	57	theme	principal	877:885	arg1	compounds					887:895	the principal compounds	873:895	the principal compounds identified	873:906	Phenolic compounds were found after pomace extraction, and catechin, gallic acid and epicatechin were the principal compounds identified.
26868559	7	58	contain	had	927:929	arg2	pomace					1002:1007	from 8.00 to 46.60 mMol Trolox/100 g pomace	965:1007	from 8.00 to 46.60 mMol Trolox/100 g pomace	965:1007	The extracts also had ABTS radical scavenging capacity (from 8.00 to 46.60 mMol Trolox/100 g pomace).
26868559	7	58	contain	had	927:929	arg1	extracts					913:920	The extracts	909:920	The extracts	909:920	The extracts also had ABTS radical scavenging capacity (from 8.00 to 46.60 mMol Trolox/100 g pomace).
26868559	7	58	contain	had	927:929	arg2	capacity					955:962	ABTS radical scavenging capacity	931:962	ABTS radical scavenging capacity (from 8.00 to 46.60 mMol Trolox/100 g pomace)	931:1008	The extracts also had ABTS radical scavenging capacity (from 8.00 to 46.60 mMol Trolox/100 g pomace).
26868559	6	59	theme	gallic	840:845	arg1	epicatechin					856:866	epicatechin	856:866	epicatechin	856:866	Phenolic compounds were found after pomace extraction, and catechin, gallic acid and epicatechin were the principal compounds identified.
26868559	6	59	theme	gallic	840:845	arg1	catechin					830:837	catechin	830:837	catechin	830:837	Phenolic compounds were found after pomace extraction, and catechin, gallic acid and epicatechin were the principal compounds identified.
26868559	6	59	theme	gallic	840:845	arg1	acid					847:850	gallic acid	840:850	gallic acid	840:850	Phenolic compounds were found after pomace extraction, and catechin, gallic acid and epicatechin were the principal compounds identified.
26868559	6	59	theme	gallic	840:845	arg1	compounds					887:895	the principal compounds	873:895	the principal compounds identified	873:906	Phenolic compounds were found after pomace extraction, and catechin, gallic acid and epicatechin were the principal compounds identified.
26868559	0	60	theme	grape	61:65	arg1	pomace					67:72	Brazilian Pinot noir grape pomace	40:72	Brazilian Pinot noir grape pomace	40:72	Antioxidant dietary fibre recovery from Brazilian Pinot noir grape pomace.
26868559	4	61	dep	Rha	567:569	arg1	Glc					587:589	Glc	587:589	Rha:Ara:Xyl:Man:Gal:Glc:GalA in a 3:32:2:13:11:20:19 M ratio	567:626	The monosaccharide composition of extracts obtained from flours were, on average, Rha:Ara:Xyl:Man:Gal:Glc:GalA in a 3:32:2:13:11:20:19 M ratio, with varying Glc:GalA ratios.
26868559	4	61	dep	Rha	567:569	arg1	Xyl					575:577	Xyl	575:577	Rha:Ara:Xyl:Man:Gal:Glc:GalA in a 3:32:2:13:11:20:19 M ratio	567:626	The monosaccharide composition of extracts obtained from flours were, on average, Rha:Ara:Xyl:Man:Gal:Glc:GalA in a 3:32:2:13:11:20:19 M ratio, with varying Glc:GalA ratios.
26868559	4	61	dep	Rha	567:569	arg1	Man					579:581	Man	579:581	Rha:Ara:Xyl:Man:Gal:Glc:GalA in a 3:32:2:13:11:20:19 M ratio	567:626	The monosaccharide composition of extracts obtained from flours were, on average, Rha:Ara:Xyl:Man:Gal:Glc:GalA in a 3:32:2:13:11:20:19 M ratio, with varying Glc:GalA ratios.
26868559	4	61	dep	Rha	567:569	arg1	GalA					591:594	GalA	591:594	Rha:Ara:Xyl:Man:Gal:Glc:GalA in a 3:32:2:13:11:20:19 M ratio	567:626	The monosaccharide composition of extracts obtained from flours were, on average, Rha:Ara:Xyl:Man:Gal:Glc:GalA in a 3:32:2:13:11:20:19 M ratio, with varying Glc:GalA ratios.
26868559	4	61	dep	Rha	567:569	arg1	Ara					571:573	Ara	571:573	Ara	571:573	The monosaccharide composition of extracts obtained from flours were, on average, Rha:Ara:Xyl:Man:Gal:Glc:GalA in a 3:32:2:13:11:20:19 M ratio, with varying Glc:GalA ratios.
26868559	4	61	dep	Rha	567:569	arg1	Gal					583:585	Gal	583:585	Gal	583:585	The monosaccharide composition of extracts obtained from flours were, on average, Rha:Ara:Xyl:Man:Gal:Glc:GalA in a 3:32:2:13:11:20:19 M ratio, with varying Glc:GalA ratios.
26868559	7	62	theme	g	1000:1000	arg1	capacity					955:962	ABTS radical scavenging capacity	931:962	ABTS radical scavenging capacity (from 8.00 to 46.60 mMol Trolox/100 g pomace)	931:1008	The extracts also had ABTS radical scavenging capacity (from 8.00 to 46.60 mMol Trolox/100 g pomace).
26868559	7	62	theme	g	1000:1000	arg1	pomace					1002:1007	from 8.00 to 46.60 mMol Trolox/100 g pomace	965:1007	from 8.00 to 46.60 mMol Trolox/100 g pomace	965:1007	The extracts also had ABTS radical scavenging capacity (from 8.00 to 46.60 mMol Trolox/100 g pomace).
24769083	0	0	theme	RAW264.7	88:95	arg1	cells					97:101	RAW264.7 cells	88:101	RAW264.7 cells	88:101	Protective effect of polysaccharides from Sargassum horneri against oxidative stress in RAW264.7 cells.
24769083	4	1	theme	intracellular	705:717	arg1	NO					719:720	intracellular NO and malonic dialdehyde (MDA) level	705:755	NO	719:720	SHS1 and SHS0.5 decreased intracellular ROS level, intracellular NO and malonic dialdehyde (MDA) level (P<0.05), restoring activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) (P<0.05), decreasing inducible nitric oxide synthase (iNOS) (P<0.05).
24769083	4	1	theme	intracellular	705:717	arg1	P<0.05					758:763	P<0.05	758:763	P<0.05	758:763	SHS1 and SHS0.5 decreased intracellular ROS level, intracellular NO and malonic dialdehyde (MDA) level (P<0.05), restoring activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) (P<0.05), decreasing inducible nitric oxide synthase (iNOS) (P<0.05).
24769083	4	1	theme	intracellular	705:717	arg1	level					698:702	intracellular ROS level	680:702	intracellular ROS level	680:702	SHS1 and SHS0.5 decreased intracellular ROS level, intracellular NO and malonic dialdehyde (MDA) level (P<0.05), restoring activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) (P<0.05), decreasing inducible nitric oxide synthase (iNOS) (P<0.05).
24769083	6	2	theme	food	1533:1536	arg1	industries					1557:1566	the functional food and pharmaceutical industries	1518:1566	the functional food and pharmaceutical industries	1518:1566	These results clearly showed that SHSc and its fractions could attenuate H2O2-induced stress injury in RAW264.7 cells, and a similar efficiency in protecting RAW264.7 cells against H2O2-induced oxidative injury between SHS1 and Vitamin C. Taken together, our findings suggested that SHS1 can effectively protect RAW264.7 cells against oxidative stress by H2O2, which might be used as a potential natural antioxidant in the functional food and pharmaceutical industries.
24769083	5	3	with	preincubation	934:946	arg1	cells					970:974	RAW264.7 cells	961:974	RAW264.7 cells	961:974	Moreover, preincubation of SHS1 with RAW264.7 cells resulted in the increase of the gene expression level of endogenous antioxidant enzymes such as MnSOD and GSH-Px (P<0.05).
24769083	4	4	theme	restoring	767:775	arg1	level					698:702	intracellular ROS level	680:702	intracellular ROS level	680:702	SHS1 and SHS0.5 decreased intracellular ROS level, intracellular NO and malonic dialdehyde (MDA) level (P<0.05), restoring activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) (P<0.05), decreasing inducible nitric oxide synthase (iNOS) (P<0.05).
24769083	4	4	theme	restoring	767:775	arg1	activities					777:786	restoring activities	767:786	restoring activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) (P<0.05)	767:861	SHS1 and SHS0.5 decreased intracellular ROS level, intracellular NO and malonic dialdehyde (MDA) level (P<0.05), restoring activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) (P<0.05), decreasing inducible nitric oxide synthase (iNOS) (P<0.05).
24769083	0	5	from	effect	11:16	arg1	horneri					52:58	Sargassum horneri	42:58	Sargassum horneri	42:58	Protective effect of polysaccharides from Sargassum horneri against oxidative stress in RAW264.7 cells.
24769083	5	6	theme	gene	1008:1011	arg1	level					1024:1028	the gene expression level	1004:1028	the gene expression level of endogenous antioxidant enzymes such as MnSOD and GSH-Px (P<0.05)	1004:1096	Moreover, preincubation of SHS1 with RAW264.7 cells resulted in the increase of the gene expression level of endogenous antioxidant enzymes such as MnSOD and GSH-Px (P<0.05).
24769083	6	7	theme	Vitamin	1327:1333	arg1	C.					1335:1336	Vitamin C.	1327:1336	Vitamin C.	1327:1336	These results clearly showed that SHSc and its fractions could attenuate H2O2-induced stress injury in RAW264.7 cells, and a similar efficiency in protecting RAW264.7 cells against H2O2-induced oxidative injury between SHS1 and Vitamin C. Taken together, our findings suggested that SHS1 can effectively protect RAW264.7 cells against oxidative stress by H2O2, which might be used as a potential natural antioxidant in the functional food and pharmaceutical industries.
24769083	3	8	theme	isolated	527:534	arg1	polysaccharides					536:550	three isolated polysaccharides	521:550	three isolated polysaccharides	521:550	After preincubation with three isolated polysaccharides, RAW264.7 cells viability were significantly restored and decreased in cellular LDH release (P<0.05).
24769083	3	9	theme	cells	562:566	arg1	viability					568:576	RAW264.7 cells viability	553:576	RAW264.7 cells viability	553:576	After preincubation with three isolated polysaccharides, RAW264.7 cells viability were significantly restored and decreased in cellular LDH release (P<0.05).
24769083	4	10	theme	intracellular	680:692	arg1	NO					719:720	intracellular NO and malonic dialdehyde (MDA) level	705:755	NO	719:720	SHS1 and SHS0.5 decreased intracellular ROS level, intracellular NO and malonic dialdehyde (MDA) level (P<0.05), restoring activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) (P<0.05), decreasing inducible nitric oxide synthase (iNOS) (P<0.05).
24769083	4	10	theme	intracellular	680:692	arg1	activities					777:786	restoring activities	767:786	restoring activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) (P<0.05)	767:861	SHS1 and SHS0.5 decreased intracellular ROS level, intracellular NO and malonic dialdehyde (MDA) level (P<0.05), restoring activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) (P<0.05), decreasing inducible nitric oxide synthase (iNOS) (P<0.05).
24769083	4	10	theme	intracellular	680:692	arg1	level					751:755	intracellular NO and malonic dialdehyde (MDA) level	705:755	level	751:755	SHS1 and SHS0.5 decreased intracellular ROS level, intracellular NO and malonic dialdehyde (MDA) level (P<0.05), restoring activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) (P<0.05), decreasing inducible nitric oxide synthase (iNOS) (P<0.05).
24769083	4	10	theme	intracellular	680:692	arg1	level					698:702	intracellular ROS level	680:702	intracellular ROS level	680:702	SHS1 and SHS0.5 decreased intracellular ROS level, intracellular NO and malonic dialdehyde (MDA) level (P<0.05), restoring activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) (P<0.05), decreasing inducible nitric oxide synthase (iNOS) (P<0.05).
24769083	1	11	theme	-induced	274:281	arg1	injury					293:298	hydrogen peroxide (H2O2)-induced oxidative injury	250:298	hydrogen peroxide (H2O2)-induced oxidative injury in RAW264.7 cells	250:316	This study was designed to investigate chemical composition and the protective effects of polysaccharides isolated from Sargassum horneri against hydrogen peroxide (H2O2)-induced oxidative injury in RAW264.7 cells.
24769083	5	12	theme	enzymes	1056:1062	arg1	level					1024:1028	the gene expression level	1004:1028	the gene expression level of endogenous antioxidant enzymes such as MnSOD and GSH-Px (P<0.05)	1004:1096	Moreover, preincubation of SHS1 with RAW264.7 cells resulted in the increase of the gene expression level of endogenous antioxidant enzymes such as MnSOD and GSH-Px (P<0.05).
24769083	5	13	theme	SHS1	951:954	arg1	preincubation					934:946	preincubation	934:946	preincubation of SHS1 with RAW264.7 cells	934:974	Moreover, preincubation of SHS1 with RAW264.7 cells resulted in the increase of the gene expression level of endogenous antioxidant enzymes such as MnSOD and GSH-Px (P<0.05).
24769083	6	14	theme	H2O2-induced	1280:1291	arg1	injury					1303:1308	H2O2-induced oxidative injury	1280:1308	H2O2-induced oxidative injury between SHS1	1280:1321	These results clearly showed that SHSc and its fractions could attenuate H2O2-induced stress injury in RAW264.7 cells, and a similar efficiency in protecting RAW264.7 cells against H2O2-induced oxidative injury between SHS1 and Vitamin C. Taken together, our findings suggested that SHS1 can effectively protect RAW264.7 cells against oxidative stress by H2O2, which might be used as a potential natural antioxidant in the functional food and pharmaceutical industries.
24769083	1	15	theme	oxidative	283:291	arg1	injury					293:298	hydrogen peroxide (H2O2)-induced oxidative injury	250:298	hydrogen peroxide (H2O2)-induced oxidative injury in RAW264.7 cells	250:316	This study was designed to investigate chemical composition and the protective effects of polysaccharides isolated from Sargassum horneri against hydrogen peroxide (H2O2)-induced oxidative injury in RAW264.7 cells.
24769083	0	16	from	horneri	52:58	arg1	polysaccharides					21:35	polysaccharides	21:35	polysaccharides from Sargassum horneri	21:58	Protective effect of polysaccharides from Sargassum horneri against oxidative stress in RAW264.7 cells.
24769083	0	16	from	horneri	52:58	arg1	effect					11:16	Protective effect	0:16	Protective effect of polysaccharides from Sargassum horneri against oxidative stress in RAW264.7 cells	0:101	Protective effect of polysaccharides from Sargassum horneri against oxidative stress in RAW264.7 cells.
24769083	6	17	theme	potential	1485:1493	arg1	antioxidant					1503:1513	a potential natural antioxidant	1483:1513	a potential natural antioxidant in the functional food and pharmaceutical industries	1483:1566	These results clearly showed that SHSc and its fractions could attenuate H2O2-induced stress injury in RAW264.7 cells, and a similar efficiency in protecting RAW264.7 cells against H2O2-induced oxidative injury between SHS1 and Vitamin C. Taken together, our findings suggested that SHS1 can effectively protect RAW264.7 cells against oxidative stress by H2O2, which might be used as a potential natural antioxidant in the functional food and pharmaceutical industries.
24769083	6	17	theme	potential	1485:1493	arg1	H2O2					1454:1457	H2O2	1454:1457	H2O2	1454:1457	These results clearly showed that SHSc and its fractions could attenuate H2O2-induced stress injury in RAW264.7 cells, and a similar efficiency in protecting RAW264.7 cells against H2O2-induced oxidative injury between SHS1 and Vitamin C. Taken together, our findings suggested that SHS1 can effectively protect RAW264.7 cells against oxidative stress by H2O2, which might be used as a potential natural antioxidant in the functional food and pharmaceutical industries.
24769083	1	18	theme	protective	172:181	arg1	effects					183:189	the protective effects	168:189	the protective effects of polysaccharides isolated from Sargassum horneri against hydrogen peroxide (H2O2)-induced oxidative injury in RAW264.7 cells	168:316	This study was designed to investigate chemical composition and the protective effects of polysaccharides isolated from Sargassum horneri against hydrogen peroxide (H2O2)-induced oxidative injury in RAW264.7 cells.
24769083	4	19	theme	peroxidase	834:843	arg1	level					698:702	intracellular ROS level	680:702	intracellular ROS level	680:702	SHS1 and SHS0.5 decreased intracellular ROS level, intracellular NO and malonic dialdehyde (MDA) level (P<0.05), restoring activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) (P<0.05), decreasing inducible nitric oxide synthase (iNOS) (P<0.05).
24769083	4	19	theme	peroxidase	834:843	arg1	activities					777:786	restoring activities	767:786	restoring activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) (P<0.05)	767:861	SHS1 and SHS0.5 decreased intracellular ROS level, intracellular NO and malonic dialdehyde (MDA) level (P<0.05), restoring activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) (P<0.05), decreasing inducible nitric oxide synthase (iNOS) (P<0.05).
24769083	3	20	theme	RAW264.7	553:560	arg1	cells					562:566	RAW264.7 cells	553:566	RAW264.7 cells viability	553:576	After preincubation with three isolated polysaccharides, RAW264.7 cells viability were significantly restored and decreased in cellular LDH release (P<0.05).
24769083	5	21	theme	endogenous	1033:1042	arg1	GSH-Px					1082:1087	GSH-Px	1082:1087	GSH-Px (P<0.05)	1082:1096	Moreover, preincubation of SHS1 with RAW264.7 cells resulted in the increase of the gene expression level of endogenous antioxidant enzymes such as MnSOD and GSH-Px (P<0.05).
24769083	5	21	theme	endogenous	1033:1042	arg1	enzymes					1056:1062	endogenous antioxidant enzymes	1033:1062	endogenous antioxidant enzymes such as MnSOD and GSH-Px (P<0.05)	1033:1096	Moreover, preincubation of SHS1 with RAW264.7 cells resulted in the increase of the gene expression level of endogenous antioxidant enzymes such as MnSOD and GSH-Px (P<0.05).
24769083	5	21	theme	endogenous	1033:1042	arg1	MnSOD					1072:1076	MnSOD	1072:1076	MnSOD	1072:1076	Moreover, preincubation of SHS1 with RAW264.7 cells resulted in the increase of the gene expression level of endogenous antioxidant enzymes such as MnSOD and GSH-Px (P<0.05).
24769083	1	22	from	injury	293:298	arg1	cells					312:316	RAW264.7 cells	303:316	RAW264.7 cells	303:316	This study was designed to investigate chemical composition and the protective effects of polysaccharides isolated from Sargassum horneri against hydrogen peroxide (H2O2)-induced oxidative injury in RAW264.7 cells.
24769083	6	23	used	used	1475:1478	arg2	antioxidant					1503:1513	a potential natural antioxidant	1483:1513	a potential natural antioxidant in the functional food and pharmaceutical industries	1483:1566	These results clearly showed that SHSc and its fractions could attenuate H2O2-induced stress injury in RAW264.7 cells, and a similar efficiency in protecting RAW264.7 cells against H2O2-induced oxidative injury between SHS1 and Vitamin C. Taken together, our findings suggested that SHS1 can effectively protect RAW264.7 cells against oxidative stress by H2O2, which might be used as a potential natural antioxidant in the functional food and pharmaceutical industries.
24769083	6	23	used	used	1475:1478	arg2	H2O2					1454:1457	H2O2	1454:1457	H2O2	1454:1457	These results clearly showed that SHSc and its fractions could attenuate H2O2-induced stress injury in RAW264.7 cells, and a similar efficiency in protecting RAW264.7 cells against H2O2-induced oxidative injury between SHS1 and Vitamin C. Taken together, our findings suggested that SHS1 can effectively protect RAW264.7 cells against oxidative stress by H2O2, which might be used as a potential natural antioxidant in the functional food and pharmaceutical industries.
24769083	0	24	theme	Protective	0:9	arg1	effect					11:16	Protective effect	0:16	Protective effect of polysaccharides from Sargassum horneri against oxidative stress in RAW264.7 cells	0:101	Protective effect of polysaccharides from Sargassum horneri against oxidative stress in RAW264.7 cells.
24769083	3	25	with	preincubation	502:514	arg1	polysaccharides					536:550	three isolated polysaccharides	521:550	three isolated polysaccharides	521:550	After preincubation with three isolated polysaccharides, RAW264.7 cells viability were significantly restored and decreased in cellular LDH release (P<0.05).
24769083	1	26	theme	polysaccharides	194:208	arg1	composition					152:162	chemical composition	143:162	chemical composition	143:162	This study was designed to investigate chemical composition and the protective effects of polysaccharides isolated from Sargassum horneri against hydrogen peroxide (H2O2)-induced oxidative injury in RAW264.7 cells.
24769083	1	26	theme	polysaccharides	194:208	arg1	effects					183:189	the protective effects	168:189	the protective effects of polysaccharides isolated from Sargassum horneri against hydrogen peroxide (H2O2)-induced oxidative injury in RAW264.7 cells	168:316	This study was designed to investigate chemical composition and the protective effects of polysaccharides isolated from Sargassum horneri against hydrogen peroxide (H2O2)-induced oxidative injury in RAW264.7 cells.
24769083	0	27	theme	polysaccharides	21:35	arg1	effect					11:16	Protective effect	0:16	Protective effect of polysaccharides from Sargassum horneri against oxidative stress in RAW264.7 cells	0:101	Protective effect of polysaccharides from Sargassum horneri against oxidative stress in RAW264.7 cells.
24769083	6	28	theme	similar	1224:1230	arg1	efficiency					1232:1241	a similar efficiency	1222:1241	a similar efficiency in protecting RAW264.7 cells against H2O2-induced oxidative injury between SHS1	1222:1321	These results clearly showed that SHSc and its fractions could attenuate H2O2-induced stress injury in RAW264.7 cells, and a similar efficiency in protecting RAW264.7 cells against H2O2-induced oxidative injury between SHS1 and Vitamin C. Taken together, our findings suggested that SHS1 can effectively protect RAW264.7 cells against oxidative stress by H2O2, which might be used as a potential natural antioxidant in the functional food and pharmaceutical industries.
24769083	2	29	theme	sulfate	420:426	arg1	ester					428:432	sulfate ester	420:432	sulfate ester	420:432	Results showed that isolated polysaccharides (SHSc) and the major fractions (SHS1, SHS0.5) contained sulfate ester, and SHS1 was high fucose-containing sulfated polysaccharide.
24769083	1	30	theme	RAW264.7	303:310	arg1	cells					312:316	RAW264.7 cells	303:316	RAW264.7 cells	303:316	This study was designed to investigate chemical composition and the protective effects of polysaccharides isolated from Sargassum horneri against hydrogen peroxide (H2O2)-induced oxidative injury in RAW264.7 cells.
24769083	6	31	theme	oxidative	1293:1301	arg1	injury					1303:1308	H2O2-induced oxidative injury	1280:1308	H2O2-induced oxidative injury between SHS1	1280:1321	These results clearly showed that SHSc and its fractions could attenuate H2O2-induced stress injury in RAW264.7 cells, and a similar efficiency in protecting RAW264.7 cells against H2O2-induced oxidative injury between SHS1 and Vitamin C. Taken together, our findings suggested that SHS1 can effectively protect RAW264.7 cells against oxidative stress by H2O2, which might be used as a potential natural antioxidant in the functional food and pharmaceutical industries.
24769083	5	32	theme	level	1024:1028	arg1	increase					992:999	the increase	988:999	the increase of the gene expression level of endogenous antioxidant enzymes such as MnSOD and GSH-Px (P<0.05)	988:1096	Moreover, preincubation of SHS1 with RAW264.7 cells resulted in the increase of the gene expression level of endogenous antioxidant enzymes such as MnSOD and GSH-Px (P<0.05).
24769083	2	33	theme	major	379:383	arg1	fractions					385:393	the major fractions	375:393	the major fractions (SHS1, SHS0.5)	375:408	Results showed that isolated polysaccharides (SHSc) and the major fractions (SHS1, SHS0.5) contained sulfate ester, and SHS1 was high fucose-containing sulfated polysaccharide.
24769083	2	34	theme	sulfated	471:478	arg1	polysaccharide					480:493	high fucose-containing sulfated polysaccharide	448:493	high fucose-containing sulfated polysaccharide	448:493	Results showed that isolated polysaccharides (SHSc) and the major fractions (SHS1, SHS0.5) contained sulfate ester, and SHS1 was high fucose-containing sulfated polysaccharide.
24769083	6	35	theme	RAW264.7	1411:1418	arg1	cells					1420:1424	RAW264.7 cells	1411:1424	RAW264.7 cells	1411:1424	These results clearly showed that SHSc and its fractions could attenuate H2O2-induced stress injury in RAW264.7 cells, and a similar efficiency in protecting RAW264.7 cells against H2O2-induced oxidative injury between SHS1 and Vitamin C. Taken together, our findings suggested that SHS1 can effectively protect RAW264.7 cells against oxidative stress by H2O2, which might be used as a potential natural antioxidant in the functional food and pharmaceutical industries.
24769083	3	36	theme	cellular	623:630	arg1	P<0.05					645:650	P<0.05	645:650	P<0.05	645:650	After preincubation with three isolated polysaccharides, RAW264.7 cells viability were significantly restored and decreased in cellular LDH release (P<0.05).
24769083	3	36	theme	cellular	623:630	arg1	release					636:642	cellular LDH release	623:642	cellular LDH release (P<0.05)	623:651	After preincubation with three isolated polysaccharides, RAW264.7 cells viability were significantly restored and decreased in cellular LDH release (P<0.05).
24769083	4	37	theme	ROS	694:696	arg1	NO					719:720	intracellular NO and malonic dialdehyde (MDA) level	705:755	NO	719:720	SHS1 and SHS0.5 decreased intracellular ROS level, intracellular NO and malonic dialdehyde (MDA) level (P<0.05), restoring activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) (P<0.05), decreasing inducible nitric oxide synthase (iNOS) (P<0.05).
24769083	4	37	theme	ROS	694:696	arg1	activities					777:786	restoring activities	767:786	restoring activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) (P<0.05)	767:861	SHS1 and SHS0.5 decreased intracellular ROS level, intracellular NO and malonic dialdehyde (MDA) level (P<0.05), restoring activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) (P<0.05), decreasing inducible nitric oxide synthase (iNOS) (P<0.05).
24769083	4	37	theme	ROS	694:696	arg1	level					751:755	intracellular NO and malonic dialdehyde (MDA) level	705:755	level	751:755	SHS1 and SHS0.5 decreased intracellular ROS level, intracellular NO and malonic dialdehyde (MDA) level (P<0.05), restoring activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) (P<0.05), decreasing inducible nitric oxide synthase (iNOS) (P<0.05).
24769083	4	37	theme	ROS	694:696	arg1	level					698:702	intracellular ROS level	680:702	intracellular ROS level	680:702	SHS1 and SHS0.5 decreased intracellular ROS level, intracellular NO and malonic dialdehyde (MDA) level (P<0.05), restoring activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) (P<0.05), decreasing inducible nitric oxide synthase (iNOS) (P<0.05).
24769083	2	38	theme	fucose-containing	453:469	arg1	polysaccharide					480:493	high fucose-containing sulfated polysaccharide	448:493	high fucose-containing sulfated polysaccharide	448:493	Results showed that isolated polysaccharides (SHSc) and the major fractions (SHS1, SHS0.5) contained sulfate ester, and SHS1 was high fucose-containing sulfated polysaccharide.
24769083	0	39	from	stress	78:83	arg1	cells					97:101	RAW264.7 cells	88:101	RAW264.7 cells	88:101	Protective effect of polysaccharides from Sargassum horneri against oxidative stress in RAW264.7 cells.
24769083	3	40	theme	LDH	632:634	arg1	P<0.05					645:650	P<0.05	645:650	P<0.05	645:650	After preincubation with three isolated polysaccharides, RAW264.7 cells viability were significantly restored and decreased in cellular LDH release (P<0.05).
24769083	3	40	theme	LDH	632:634	arg1	release					636:642	cellular LDH release	623:642	cellular LDH release (P<0.05)	623:651	After preincubation with three isolated polysaccharides, RAW264.7 cells viability were significantly restored and decreased in cellular LDH release (P<0.05).
24769083	2	41	theme	SHS0.5	402:407	arg1	SHS1					396:399	SHS1	396:399	SHS1	396:399	Results showed that isolated polysaccharides (SHSc) and the major fractions (SHS1, SHS0.5) contained sulfate ester, and SHS1 was high fucose-containing sulfated polysaccharide.
24769083	2	42	theme	high	448:451	arg1	polysaccharide					480:493	high fucose-containing sulfated polysaccharide	448:493	high fucose-containing sulfated polysaccharide	448:493	Results showed that isolated polysaccharides (SHSc) and the major fractions (SHS1, SHS0.5) contained sulfate ester, and SHS1 was high fucose-containing sulfated polysaccharide.
24769083	4	43	theme	inducible	875:883	arg1	iNOS					908:911	iNOS	908:911	iNOS	908:911	SHS1 and SHS0.5 decreased intracellular ROS level, intracellular NO and malonic dialdehyde (MDA) level (P<0.05), restoring activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) (P<0.05), decreasing inducible nitric oxide synthase (iNOS) (P<0.05).
24769083	4	43	theme	inducible	875:883	arg1	P<0.05					915:920	P<0.05	915:920	P<0.05	915:920	SHS1 and SHS0.5 decreased intracellular ROS level, intracellular NO and malonic dialdehyde (MDA) level (P<0.05), restoring activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) (P<0.05), decreasing inducible nitric oxide synthase (iNOS) (P<0.05).
24769083	4	43	theme	inducible	875:883	arg1	synthase					898:905	inducible nitric oxide synthase	875:905	inducible nitric oxide synthase (iNOS) (P<0.05)	875:921	SHS1 and SHS0.5 decreased intracellular ROS level, intracellular NO and malonic dialdehyde (MDA) level (P<0.05), restoring activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) (P<0.05), decreasing inducible nitric oxide synthase (iNOS) (P<0.05).
24769083	5	44	theme	antioxidant	1044:1054	arg1	GSH-Px					1082:1087	GSH-Px	1082:1087	GSH-Px (P<0.05)	1082:1096	Moreover, preincubation of SHS1 with RAW264.7 cells resulted in the increase of the gene expression level of endogenous antioxidant enzymes such as MnSOD and GSH-Px (P<0.05).
24769083	5	44	theme	antioxidant	1044:1054	arg1	enzymes					1056:1062	endogenous antioxidant enzymes	1033:1062	endogenous antioxidant enzymes such as MnSOD and GSH-Px (P<0.05)	1033:1096	Moreover, preincubation of SHS1 with RAW264.7 cells resulted in the increase of the gene expression level of endogenous antioxidant enzymes such as MnSOD and GSH-Px (P<0.05).
24769083	5	44	theme	antioxidant	1044:1054	arg1	MnSOD					1072:1076	MnSOD	1072:1076	MnSOD	1072:1076	Moreover, preincubation of SHS1 with RAW264.7 cells resulted in the increase of the gene expression level of endogenous antioxidant enzymes such as MnSOD and GSH-Px (P<0.05).
24769083	4	45	theme	glutathione	822:832	arg1	GSH-Px					846:851	GSH-Px	846:851	GSH-Px	846:851	SHS1 and SHS0.5 decreased intracellular ROS level, intracellular NO and malonic dialdehyde (MDA) level (P<0.05), restoring activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) (P<0.05), decreasing inducible nitric oxide synthase (iNOS) (P<0.05).
24769083	4	45	theme	glutathione	822:832	arg1	P<0.05					855:860	P<0.05	855:860	P<0.05	855:860	SHS1 and SHS0.5 decreased intracellular ROS level, intracellular NO and malonic dialdehyde (MDA) level (P<0.05), restoring activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) (P<0.05), decreasing inducible nitric oxide synthase (iNOS) (P<0.05).
24769083	4	45	theme	glutathione	822:832	arg1	peroxidase					834:843	glutathione peroxidase	822:843	glutathione peroxidase (GSH-Px) (P<0.05)	822:861	SHS1 and SHS0.5 decreased intracellular ROS level, intracellular NO and malonic dialdehyde (MDA) level (P<0.05), restoring activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) (P<0.05), decreasing inducible nitric oxide synthase (iNOS) (P<0.05).
24769083	6	46	theme	pharmaceutical	1542:1555	arg1	industries					1557:1566	the functional food and pharmaceutical industries	1518:1566	the functional food and pharmaceutical industries	1518:1566	These results clearly showed that SHSc and its fractions could attenuate H2O2-induced stress injury in RAW264.7 cells, and a similar efficiency in protecting RAW264.7 cells against H2O2-induced oxidative injury between SHS1 and Vitamin C. Taken together, our findings suggested that SHS1 can effectively protect RAW264.7 cells against oxidative stress by H2O2, which might be used as a potential natural antioxidant in the functional food and pharmaceutical industries.
24769083	4	47	theme	dialdehyde	734:743	arg1	level					751:755	intracellular NO and malonic dialdehyde (MDA) level	705:755	level	751:755	SHS1 and SHS0.5 decreased intracellular ROS level, intracellular NO and malonic dialdehyde (MDA) level (P<0.05), restoring activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) (P<0.05), decreasing inducible nitric oxide synthase (iNOS) (P<0.05).
24769083	4	47	theme	dialdehyde	734:743	arg1	level					698:702	intracellular ROS level	680:702	intracellular ROS level	680:702	SHS1 and SHS0.5 decreased intracellular ROS level, intracellular NO and malonic dialdehyde (MDA) level (P<0.05), restoring activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) (P<0.05), decreasing inducible nitric oxide synthase (iNOS) (P<0.05).
24769083	6	48	theme	RAW264.7	1257:1264	arg1	cells					1266:1270	RAW264.7 cells	1257:1270	RAW264.7 cells	1257:1270	These results clearly showed that SHSc and its fractions could attenuate H2O2-induced stress injury in RAW264.7 cells, and a similar efficiency in protecting RAW264.7 cells against H2O2-induced oxidative injury between SHS1 and Vitamin C. Taken together, our findings suggested that SHS1 can effectively protect RAW264.7 cells against oxidative stress by H2O2, which might be used as a potential natural antioxidant in the functional food and pharmaceutical industries.
24769083	4	49	theme	malonic	726:732	arg1	level					751:755	intracellular NO and malonic dialdehyde (MDA) level	705:755	level	751:755	SHS1 and SHS0.5 decreased intracellular ROS level, intracellular NO and malonic dialdehyde (MDA) level (P<0.05), restoring activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) (P<0.05), decreasing inducible nitric oxide synthase (iNOS) (P<0.05).
24769083	4	49	theme	malonic	726:732	arg1	level					698:702	intracellular ROS level	680:702	intracellular ROS level	680:702	SHS1 and SHS0.5 decreased intracellular ROS level, intracellular NO and malonic dialdehyde (MDA) level (P<0.05), restoring activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) (P<0.05), decreasing inducible nitric oxide synthase (iNOS) (P<0.05).
24769083	6	50	theme	natural	1495:1501	arg1	antioxidant					1503:1513	a potential natural antioxidant	1483:1513	a potential natural antioxidant in the functional food and pharmaceutical industries	1483:1566	These results clearly showed that SHSc and its fractions could attenuate H2O2-induced stress injury in RAW264.7 cells, and a similar efficiency in protecting RAW264.7 cells against H2O2-induced oxidative injury between SHS1 and Vitamin C. Taken together, our findings suggested that SHS1 can effectively protect RAW264.7 cells against oxidative stress by H2O2, which might be used as a potential natural antioxidant in the functional food and pharmaceutical industries.
24769083	6	50	theme	natural	1495:1501	arg1	H2O2					1454:1457	H2O2	1454:1457	H2O2	1454:1457	These results clearly showed that SHSc and its fractions could attenuate H2O2-induced stress injury in RAW264.7 cells, and a similar efficiency in protecting RAW264.7 cells against H2O2-induced oxidative injury between SHS1 and Vitamin C. Taken together, our findings suggested that SHS1 can effectively protect RAW264.7 cells against oxidative stress by H2O2, which might be used as a potential natural antioxidant in the functional food and pharmaceutical industries.
24769083	4	51	theme	oxide	892:896	arg1	iNOS					908:911	iNOS	908:911	iNOS	908:911	SHS1 and SHS0.5 decreased intracellular ROS level, intracellular NO and malonic dialdehyde (MDA) level (P<0.05), restoring activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) (P<0.05), decreasing inducible nitric oxide synthase (iNOS) (P<0.05).
24769083	4	51	theme	oxide	892:896	arg1	P<0.05					915:920	P<0.05	915:920	P<0.05	915:920	SHS1 and SHS0.5 decreased intracellular ROS level, intracellular NO and malonic dialdehyde (MDA) level (P<0.05), restoring activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) (P<0.05), decreasing inducible nitric oxide synthase (iNOS) (P<0.05).
24769083	4	51	theme	oxide	892:896	arg1	synthase					898:905	inducible nitric oxide synthase	875:905	inducible nitric oxide synthase (iNOS) (P<0.05)	875:921	SHS1 and SHS0.5 decreased intracellular ROS level, intracellular NO and malonic dialdehyde (MDA) level (P<0.05), restoring activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) (P<0.05), decreasing inducible nitric oxide synthase (iNOS) (P<0.05).
24769083	1	52	attach	isolated	210:217	arg2	polysaccharides					194:208	polysaccharides	194:208	polysaccharides isolated from Sargassum horneri	194:240	This study was designed to investigate chemical composition and the protective effects of polysaccharides isolated from Sargassum horneri against hydrogen peroxide (H2O2)-induced oxidative injury in RAW264.7 cells.
24769083	1	52	attach	isolated	210:217	arg1	horneri					234:240	Sargassum horneri	224:240	Sargassum horneri	224:240	This study was designed to investigate chemical composition and the protective effects of polysaccharides isolated from Sargassum horneri against hydrogen peroxide (H2O2)-induced oxidative injury in RAW264.7 cells.
24769083	4	53	theme	superoxide	791:800	arg1	dismutase					802:810	superoxide dismutase	791:810	superoxide dismutase (SOD)	791:816	SHS1 and SHS0.5 decreased intracellular ROS level, intracellular NO and malonic dialdehyde (MDA) level (P<0.05), restoring activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) (P<0.05), decreasing inducible nitric oxide synthase (iNOS) (P<0.05).
24769083	4	53	theme	superoxide	791:800	arg1	SOD					813:815	SOD	813:815	SOD	813:815	SHS1 and SHS0.5 decreased intracellular ROS level, intracellular NO and malonic dialdehyde (MDA) level (P<0.05), restoring activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) (P<0.05), decreasing inducible nitric oxide synthase (iNOS) (P<0.05).
24769083	6	54	theme	stress	1185:1190	arg1	injury					1192:1197	H2O2-induced stress injury	1172:1197	H2O2-induced stress injury in RAW264.7 cells	1172:1215	These results clearly showed that SHSc and its fractions could attenuate H2O2-induced stress injury in RAW264.7 cells, and a similar efficiency in protecting RAW264.7 cells against H2O2-induced oxidative injury between SHS1 and Vitamin C. Taken together, our findings suggested that SHS1 can effectively protect RAW264.7 cells against oxidative stress by H2O2, which might be used as a potential natural antioxidant in the functional food and pharmaceutical industries.
24769083	4	55	theme	MDA	746:748	arg1	level					751:755	intracellular NO and malonic dialdehyde (MDA) level	705:755	level	751:755	SHS1 and SHS0.5 decreased intracellular ROS level, intracellular NO and malonic dialdehyde (MDA) level (P<0.05), restoring activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) (P<0.05), decreasing inducible nitric oxide synthase (iNOS) (P<0.05).
24769083	4	55	theme	MDA	746:748	arg1	level					698:702	intracellular ROS level	680:702	intracellular ROS level	680:702	SHS1 and SHS0.5 decreased intracellular ROS level, intracellular NO and malonic dialdehyde (MDA) level (P<0.05), restoring activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) (P<0.05), decreasing inducible nitric oxide synthase (iNOS) (P<0.05).
24769083	6	56	theme	RAW264.7	1202:1209	arg1	cells					1211:1215	RAW264.7 cells	1202:1215	RAW264.7 cells	1202:1215	These results clearly showed that SHSc and its fractions could attenuate H2O2-induced stress injury in RAW264.7 cells, and a similar efficiency in protecting RAW264.7 cells against H2O2-induced oxidative injury between SHS1 and Vitamin C. Taken together, our findings suggested that SHS1 can effectively protect RAW264.7 cells against oxidative stress by H2O2, which might be used as a potential natural antioxidant in the functional food and pharmaceutical industries.
24769083	4	57	theme	nitric	885:890	arg1	iNOS					908:911	iNOS	908:911	iNOS	908:911	SHS1 and SHS0.5 decreased intracellular ROS level, intracellular NO and malonic dialdehyde (MDA) level (P<0.05), restoring activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) (P<0.05), decreasing inducible nitric oxide synthase (iNOS) (P<0.05).
24769083	4	57	theme	nitric	885:890	arg1	P<0.05					915:920	P<0.05	915:920	P<0.05	915:920	SHS1 and SHS0.5 decreased intracellular ROS level, intracellular NO and malonic dialdehyde (MDA) level (P<0.05), restoring activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) (P<0.05), decreasing inducible nitric oxide synthase (iNOS) (P<0.05).
24769083	4	57	theme	nitric	885:890	arg1	synthase					898:905	inducible nitric oxide synthase	875:905	inducible nitric oxide synthase (iNOS) (P<0.05)	875:921	SHS1 and SHS0.5 decreased intracellular ROS level, intracellular NO and malonic dialdehyde (MDA) level (P<0.05), restoring activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) (P<0.05), decreasing inducible nitric oxide synthase (iNOS) (P<0.05).
24769083	2	58	contain	contained	410:418	arg2	ester					428:432	sulfate ester	420:432	sulfate ester	420:432	Results showed that isolated polysaccharides (SHSc) and the major fractions (SHS1, SHS0.5) contained sulfate ester, and SHS1 was high fucose-containing sulfated polysaccharide.
24769083	2	58	contain	contained	410:418	arg1	fractions					385:393	the major fractions	375:393	the major fractions (SHS1, SHS0.5)	375:408	Results showed that isolated polysaccharides (SHSc) and the major fractions (SHS1, SHS0.5) contained sulfate ester, and SHS1 was high fucose-containing sulfated polysaccharide.
24769083	2	58	contain	contained	410:418	arg1	SHSc					365:368	SHSc	365:368	SHSc	365:368	Results showed that isolated polysaccharides (SHSc) and the major fractions (SHS1, SHS0.5) contained sulfate ester, and SHS1 was high fucose-containing sulfated polysaccharide.
24769083	2	58	contain	contained	410:418	arg1	polysaccharides					348:362	isolated polysaccharides	339:362	isolated polysaccharides (SHSc)	339:369	Results showed that isolated polysaccharides (SHSc) and the major fractions (SHS1, SHS0.5) contained sulfate ester, and SHS1 was high fucose-containing sulfated polysaccharide.
24769083	2	59	dep	fractions	385:393	arg1	SHS1					396:399	SHS1	396:399	SHS1	396:399	Results showed that isolated polysaccharides (SHSc) and the major fractions (SHS1, SHS0.5) contained sulfate ester, and SHS1 was high fucose-containing sulfated polysaccharide.
24769083	6	60	theme	functional	1522:1531	arg1	industries					1557:1566	the functional food and pharmaceutical industries	1518:1566	the functional food and pharmaceutical industries	1518:1566	These results clearly showed that SHSc and its fractions could attenuate H2O2-induced stress injury in RAW264.7 cells, and a similar efficiency in protecting RAW264.7 cells against H2O2-induced oxidative injury between SHS1 and Vitamin C. Taken together, our findings suggested that SHS1 can effectively protect RAW264.7 cells against oxidative stress by H2O2, which might be used as a potential natural antioxidant in the functional food and pharmaceutical industries.
24769083	2	61	theme	isolated	339:346	arg1	SHSc					365:368	SHSc	365:368	SHSc	365:368	Results showed that isolated polysaccharides (SHSc) and the major fractions (SHS1, SHS0.5) contained sulfate ester, and SHS1 was high fucose-containing sulfated polysaccharide.
24769083	2	61	theme	isolated	339:346	arg1	polysaccharides					348:362	isolated polysaccharides	339:362	isolated polysaccharides (SHSc)	339:369	Results showed that isolated polysaccharides (SHSc) and the major fractions (SHS1, SHS0.5) contained sulfate ester, and SHS1 was high fucose-containing sulfated polysaccharide.
24769083	6	62	from	injury	1192:1197	arg1	cells					1211:1215	RAW264.7 cells	1202:1215	RAW264.7 cells	1202:1215	These results clearly showed that SHSc and its fractions could attenuate H2O2-induced stress injury in RAW264.7 cells, and a similar efficiency in protecting RAW264.7 cells against H2O2-induced oxidative injury between SHS1 and Vitamin C. Taken together, our findings suggested that SHS1 can effectively protect RAW264.7 cells against oxidative stress by H2O2, which might be used as a potential natural antioxidant in the functional food and pharmaceutical industries.
24769083	1	63	theme	hydrogen	250:257	arg1	peroxide					259:266	hydrogen peroxide	250:266	hydrogen peroxide (H2O2)	250:273	This study was designed to investigate chemical composition and the protective effects of polysaccharides isolated from Sargassum horneri against hydrogen peroxide (H2O2)-induced oxidative injury in RAW264.7 cells.
24769083	1	63	theme	hydrogen	250:257	arg1	H2O2					269:272	H2O2	269:272	H2O2	269:272	This study was designed to investigate chemical composition and the protective effects of polysaccharides isolated from Sargassum horneri against hydrogen peroxide (H2O2)-induced oxidative injury in RAW264.7 cells.
24769083	5	64	theme	expression	1013:1022	arg1	level					1024:1028	the gene expression level	1004:1028	the gene expression level of endogenous antioxidant enzymes such as MnSOD and GSH-Px (P<0.05)	1004:1096	Moreover, preincubation of SHS1 with RAW264.7 cells resulted in the increase of the gene expression level of endogenous antioxidant enzymes such as MnSOD and GSH-Px (P<0.05).
24769083	5	65	theme	RAW264.7	961:968	arg1	cells					970:974	RAW264.7 cells	961:974	RAW264.7 cells	961:974	Moreover, preincubation of SHS1 with RAW264.7 cells resulted in the increase of the gene expression level of endogenous antioxidant enzymes such as MnSOD and GSH-Px (P<0.05).
24769083	6	66	theme	H2O2-induced	1172:1183	arg1	injury					1192:1197	H2O2-induced stress injury	1172:1197	H2O2-induced stress injury in RAW264.7 cells	1172:1215	These results clearly showed that SHSc and its fractions could attenuate H2O2-induced stress injury in RAW264.7 cells, and a similar efficiency in protecting RAW264.7 cells against H2O2-induced oxidative injury between SHS1 and Vitamin C. Taken together, our findings suggested that SHS1 can effectively protect RAW264.7 cells against oxidative stress by H2O2, which might be used as a potential natural antioxidant in the functional food and pharmaceutical industries.
24769083	1	67	theme	chemical	143:150	arg1	composition					152:162	chemical composition	143:162	chemical composition	143:162	This study was designed to investigate chemical composition and the protective effects of polysaccharides isolated from Sargassum horneri against hydrogen peroxide (H2O2)-induced oxidative injury in RAW264.7 cells.
24769083	0	68	theme	oxidative	68:76	arg1	stress					78:83	oxidative stress	68:83	oxidative stress in RAW264.7 cells	68:101	Protective effect of polysaccharides from Sargassum horneri against oxidative stress in RAW264.7 cells.
24769083	4	69	theme	dismutase	802:810	arg1	level					698:702	intracellular ROS level	680:702	intracellular ROS level	680:702	SHS1 and SHS0.5 decreased intracellular ROS level, intracellular NO and malonic dialdehyde (MDA) level (P<0.05), restoring activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) (P<0.05), decreasing inducible nitric oxide synthase (iNOS) (P<0.05).
24769083	4	69	theme	dismutase	802:810	arg1	activities					777:786	restoring activities	767:786	restoring activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) (P<0.05)	767:861	SHS1 and SHS0.5 decreased intracellular ROS level, intracellular NO and malonic dialdehyde (MDA) level (P<0.05), restoring activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) (P<0.05), decreasing inducible nitric oxide synthase (iNOS) (P<0.05).
24769083	6	70	from	antioxidant	1503:1513	arg1	industries					1557:1566	the functional food and pharmaceutical industries	1518:1566	the functional food and pharmaceutical industries	1518:1566	These results clearly showed that SHSc and its fractions could attenuate H2O2-induced stress injury in RAW264.7 cells, and a similar efficiency in protecting RAW264.7 cells against H2O2-induced oxidative injury between SHS1 and Vitamin C. Taken together, our findings suggested that SHS1 can effectively protect RAW264.7 cells against oxidative stress by H2O2, which might be used as a potential natural antioxidant in the functional food and pharmaceutical industries.
24769083	6	71	theme	oxidative	1434:1442	arg1	stress					1444:1449	oxidative stress	1434:1449	oxidative stress	1434:1449	These results clearly showed that SHSc and its fractions could attenuate H2O2-induced stress injury in RAW264.7 cells, and a similar efficiency in protecting RAW264.7 cells against H2O2-induced oxidative injury between SHS1 and Vitamin C. Taken together, our findings suggested that SHS1 can effectively protect RAW264.7 cells against oxidative stress by H2O2, which might be used as a potential natural antioxidant in the functional food and pharmaceutical industries.
26616946	8	0	theme	BTPEPS	1241:1246	arg1	microstructures					1203:1217	microstructures	1203:1217	microstructures	1203:1217	Heat treatments applied before extraction affected the properties, composition and microstructures of FDPEPS, ODPEPS and BTPEPS.
26616946	8	0	theme	BTPEPS	1241:1246	arg1	composition					1187:1197	composition	1187:1197	composition	1187:1197	Heat treatments applied before extraction affected the properties, composition and microstructures of FDPEPS, ODPEPS and BTPEPS.
26616946	8	0	theme	BTPEPS	1241:1246	arg1	properties					1175:1184	the properties	1171:1184	the properties	1171:1184	Heat treatments applied before extraction affected the properties, composition and microstructures of FDPEPS, ODPEPS and BTPEPS.
26616946	3	1	dep	galactose	576:584	arg1	%					591:591	5.34%	587:591	5.34%	587:591	High performance liquid chromatography (HPLC) identified that FDPEPS, ODPEPS, and BTPEPS were composed of mannose (5.75%, 5.52%, 6.97%), glucose (88.90%, 89.31%, 87.68%) and galactose (5.34%, 5.17%, 5.35%).
26616946	3	1	dep	galactose	576:584	arg1	%					598:598	5.17%	594:598	5.17%	594:598	High performance liquid chromatography (HPLC) identified that FDPEPS, ODPEPS, and BTPEPS were composed of mannose (5.75%, 5.52%, 6.97%), glucose (88.90%, 89.31%, 87.68%) and galactose (5.34%, 5.17%, 5.35%).
26616946	3	1	dep	galactose	576:584	arg1	%					605:605	5.35%	601:605	5.35%	601:605	High performance liquid chromatography (HPLC) identified that FDPEPS, ODPEPS, and BTPEPS were composed of mannose (5.75%, 5.52%, 6.97%), glucose (88.90%, 89.31%, 87.68%) and galactose (5.34%, 5.17%, 5.35%).
26616946	4	2	theme	typical	642:648	arg1	bands					671:675	typical saccharic absorption bands	642:675	typical saccharic absorption bands	642:675	All three polysaccharides showed typical saccharic absorption bands in FT-IR.
26616946	6	3	located	observed	893:900	arg2	aggregates					854:863	Denser aggregates	847:863	Denser aggregates	847:863	Denser aggregates and larger serum pores were observed in confocal micrographs of soymilk added with ODPEPS.
26616946	6	3	located	observed	893:900	arg1	micrographs					914:924	confocal micrographs	905:924	confocal micrographs of soymilk added with ODPEPS	905:953	Denser aggregates and larger serum pores were observed in confocal micrographs of soymilk added with ODPEPS.
26616946	6	3	located	observed	893:900	arg2	pores					882:886	larger serum pores	869:886	larger serum pores	869:886	Denser aggregates and larger serum pores were observed in confocal micrographs of soymilk added with ODPEPS.
26616946	6	4	theme	larger	869:874	arg1	pores					882:886	larger serum pores	869:886	larger serum pores	869:886	Denser aggregates and larger serum pores were observed in confocal micrographs of soymilk added with ODPEPS.
26616946	0	5	theme	heat	104:107	arg1	treatments					109:118	heat treatments	104:118	heat treatments	104:118	Characterization, antioxidative and bifidogenic effects of polysaccharides from Pleurotus eryngii after heat treatments.
26616946	1	6	dep	freeze-dried-	157:169	arg1	Pleurotus					204:212	Pleurotus	204:212	Pleurotus	204:212	Polysaccharides were extracted from freeze-dried-, oven-dried- and boiling-treated Pleurotus eryngii (FDPEPS, ODPEPS and BTPEPS).
26616946	2	7	theme	total	296:300	arg1	polysaccharides					302:316	total polysaccharides	296:316	total polysaccharides	296:316	Yield of FDPEPS and its total carbohydrates, total polysaccharides, reducing sugars, phenolics and protein content were higher than ODPEPS and BTPEPS.
26616946	7	8	from	Viability	956:964	arg1	soymilk					995:1001	soymilk	995:1001	soymilk added with polysaccharides	995:1028	Viability of Bifidobacterium longum in soymilk added with polysaccharides was significantly higher (p<0.01) than those without polysaccharides during fermentation.
26616946	7	9	theme	longum	985:990	arg1	Viability					956:964	Viability	956:964	Viability of Bifidobacterium longum in soymilk added with polysaccharides	956:1028	Viability of Bifidobacterium longum in soymilk added with polysaccharides was significantly higher (p<0.01) than those without polysaccharides during fermentation.
26616946	6	10	theme	soymilk	929:935	arg1	micrographs					914:924	confocal micrographs	905:924	confocal micrographs of soymilk added with ODPEPS	905:953	Denser aggregates and larger serum pores were observed in confocal micrographs of soymilk added with ODPEPS.
26616946	4	11	theme	absorption	660:669	arg1	bands					671:675	typical saccharic absorption bands	642:675	typical saccharic absorption bands	642:675	All three polysaccharides showed typical saccharic absorption bands in FT-IR.
26616946	5	12	theme	ferric	743:748	arg1	FRAP					778:781	FRAP	778:781	FRAP	778:781	The FDPEPS showed the highest antioxidant activities in ferric reducing antioxidant power (FRAP), ABTS, superoxide anion and hydroxyl radical scavenging tests.
26616946	5	12	theme	ferric	743:748	arg1	power					771:775	ferric reducing antioxidant power	743:775	ferric reducing antioxidant power (FRAP)	743:782	The FDPEPS showed the highest antioxidant activities in ferric reducing antioxidant power (FRAP), ABTS, superoxide anion and hydroxyl radical scavenging tests.
26616946	0	13	from	effects	48:54	arg1	Pleurotus					80:88	Pleurotus	80:88	Pleurotus	80:88	Characterization, antioxidative and bifidogenic effects of polysaccharides from Pleurotus eryngii after heat treatments.
26616946	2	14	theme	carbohydrates	281:293	arg1	Yield					251:255	Yield	251:255	Yield of FDPEPS and its total carbohydrates, total polysaccharides, reducing sugars, phenolics and protein content	251:364	Yield of FDPEPS and its total carbohydrates, total polysaccharides, reducing sugars, phenolics and protein content were higher than ODPEPS and BTPEPS.
26616946	3	15	dep	glucose	539:545	arg1	%					569:569	87.68%	564:569	87.68%	564:569	High performance liquid chromatography (HPLC) identified that FDPEPS, ODPEPS, and BTPEPS were composed of mannose (5.75%, 5.52%, 6.97%), glucose (88.90%, 89.31%, 87.68%) and galactose (5.34%, 5.17%, 5.35%).
26616946	3	15	dep	glucose	539:545	arg1	%					553:553	88.90%	548:553	88.90%	548:553	High performance liquid chromatography (HPLC) identified that FDPEPS, ODPEPS, and BTPEPS were composed of mannose (5.75%, 5.52%, 6.97%), glucose (88.90%, 89.31%, 87.68%) and galactose (5.34%, 5.17%, 5.35%).
26616946	3	15	dep	glucose	539:545	arg1	%					561:561	89.31%	556:561	89.31%	556:561	High performance liquid chromatography (HPLC) identified that FDPEPS, ODPEPS, and BTPEPS were composed of mannose (5.75%, 5.52%, 6.97%), glucose (88.90%, 89.31%, 87.68%) and galactose (5.34%, 5.17%, 5.35%).
26616946	7	16	dep	higher	1048:1053	arg1	p<0.01					1056:1061	p<0.01	1056:1061	p<0.01	1056:1061	Viability of Bifidobacterium longum in soymilk added with polysaccharides was significantly higher (p<0.01) than those without polysaccharides during fermentation.
26616946	5	17	theme	antioxidant	759:769	arg1	FRAP					778:781	FRAP	778:781	FRAP	778:781	The FDPEPS showed the highest antioxidant activities in ferric reducing antioxidant power (FRAP), ABTS, superoxide anion and hydroxyl radical scavenging tests.
26616946	5	17	theme	antioxidant	759:769	arg1	power					771:775	ferric reducing antioxidant power	743:775	ferric reducing antioxidant power (FRAP)	743:782	The FDPEPS showed the highest antioxidant activities in ferric reducing antioxidant power (FRAP), ABTS, superoxide anion and hydroxyl radical scavenging tests.
26616946	3	18	theme	High	402:405	arg1	HPLC					442:445	HPLC	442:445	HPLC	442:445	High performance liquid chromatography (HPLC) identified that FDPEPS, ODPEPS, and BTPEPS were composed of mannose (5.75%, 5.52%, 6.97%), glucose (88.90%, 89.31%, 87.68%) and galactose (5.34%, 5.17%, 5.35%).
26616946	3	18	theme	High	402:405	arg1	chromatography					426:439	High performance liquid chromatography	402:439	High performance liquid chromatography (HPLC)	402:446	High performance liquid chromatography (HPLC) identified that FDPEPS, ODPEPS, and BTPEPS were composed of mannose (5.75%, 5.52%, 6.97%), glucose (88.90%, 89.31%, 87.68%) and galactose (5.34%, 5.17%, 5.35%).
26616946	3	19	dep	mannose	508:514	arg1	%					521:521	5.75%	517:521	5.75%	517:521	High performance liquid chromatography (HPLC) identified that FDPEPS, ODPEPS, and BTPEPS were composed of mannose (5.75%, 5.52%, 6.97%), glucose (88.90%, 89.31%, 87.68%) and galactose (5.34%, 5.17%, 5.35%).
26616946	3	19	dep	mannose	508:514	arg1	%					528:528	5.52%	524:528	5.52%	524:528	High performance liquid chromatography (HPLC) identified that FDPEPS, ODPEPS, and BTPEPS were composed of mannose (5.75%, 5.52%, 6.97%), glucose (88.90%, 89.31%, 87.68%) and galactose (5.34%, 5.17%, 5.35%).
26616946	3	19	dep	mannose	508:514	arg1	%					535:535	6.97%	531:535	6.97%	531:535	High performance liquid chromatography (HPLC) identified that FDPEPS, ODPEPS, and BTPEPS were composed of mannose (5.75%, 5.52%, 6.97%), glucose (88.90%, 89.31%, 87.68%) and galactose (5.34%, 5.17%, 5.35%).
26616946	6	20	theme	Denser	847:852	arg1	aggregates					854:863	Denser aggregates	847:863	Denser aggregates	847:863	Denser aggregates and larger serum pores were observed in confocal micrographs of soymilk added with ODPEPS.
26616946	2	21	theme	reducing	319:326	arg1	sugars					328:333	reducing sugars	319:333	reducing sugars	319:333	Yield of FDPEPS and its total carbohydrates, total polysaccharides, reducing sugars, phenolics and protein content were higher than ODPEPS and BTPEPS.
26616946	5	22	theme	highest	709:715	arg1	activities					729:738	the highest antioxidant activities	705:738	the highest antioxidant activities in ferric reducing antioxidant power (FRAP), ABTS, superoxide anion and hydroxyl radical scavenging tests	705:844	The FDPEPS showed the highest antioxidant activities in ferric reducing antioxidant power (FRAP), ABTS, superoxide anion and hydroxyl radical scavenging tests.
26616946	0	23	theme	antioxidative	18:30	arg1	effects					48:54	antioxidative and bifidogenic effects	18:54	antioxidative and bifidogenic effects of polysaccharides from Pleurotus eryngii after heat treatments	18:118	Characterization, antioxidative and bifidogenic effects of polysaccharides from Pleurotus eryngii after heat treatments.
26616946	0	23	theme	antioxidative	18:30	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization, antioxidative and bifidogenic effects of polysaccharides from Pleurotus eryngii after heat treatments.
26616946	5	24	theme	hydroxyl	812:819	arg1	radical					821:827	hydroxyl radical	812:827	hydroxyl radical	812:827	The FDPEPS showed the highest antioxidant activities in ferric reducing antioxidant power (FRAP), ABTS, superoxide anion and hydroxyl radical scavenging tests.
26616946	5	25	theme	scavenging	829:838	arg1	tests					840:844	scavenging tests	829:844	scavenging tests	829:844	The FDPEPS showed the highest antioxidant activities in ferric reducing antioxidant power (FRAP), ABTS, superoxide anion and hydroxyl radical scavenging tests.
26616946	5	26	theme	antioxidant	717:727	arg1	activities					729:738	the highest antioxidant activities	705:738	the highest antioxidant activities in ferric reducing antioxidant power (FRAP), ABTS, superoxide anion and hydroxyl radical scavenging tests	705:844	The FDPEPS showed the highest antioxidant activities in ferric reducing antioxidant power (FRAP), ABTS, superoxide anion and hydroxyl radical scavenging tests.
26616946	8	27	theme	Heat	1120:1123	arg1	treatments					1125:1134	Heat treatments	1120:1134	Heat treatments applied before extraction	1120:1160	Heat treatments applied before extraction affected the properties, composition and microstructures of FDPEPS, ODPEPS and BTPEPS.
26616946	0	28	theme	bifidogenic	36:46	arg1	effects					48:54	antioxidative and bifidogenic effects	18:54	antioxidative and bifidogenic effects of polysaccharides from Pleurotus eryngii after heat treatments	18:118	Characterization, antioxidative and bifidogenic effects of polysaccharides from Pleurotus eryngii after heat treatments.
26616946	0	28	theme	bifidogenic	36:46	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization, antioxidative and bifidogenic effects of polysaccharides from Pleurotus eryngii after heat treatments.
26616946	6	29	theme	serum	876:880	arg1	pores					882:886	larger serum pores	869:886	larger serum pores	869:886	Denser aggregates and larger serum pores were observed in confocal micrographs of soymilk added with ODPEPS.
26616946	0	30	from	Pleurotus	80:88	arg1	effects					48:54	antioxidative and bifidogenic effects	18:54	antioxidative and bifidogenic effects of polysaccharides from Pleurotus eryngii after heat treatments	18:118	Characterization, antioxidative and bifidogenic effects of polysaccharides from Pleurotus eryngii after heat treatments.
26616946	0	30	from	Pleurotus	80:88	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization, antioxidative and bifidogenic effects of polysaccharides from Pleurotus eryngii after heat treatments.
26616946	0	30	from	Pleurotus	80:88	arg1	polysaccharides					59:73	polysaccharides	59:73	polysaccharides from Pleurotus eryngii	59:96	Characterization, antioxidative and bifidogenic effects of polysaccharides from Pleurotus eryngii after heat treatments.
26616946	5	31	dep	power	771:775	arg1	tests					840:844	scavenging tests	829:844	scavenging tests	829:844	The FDPEPS showed the highest antioxidant activities in ferric reducing antioxidant power (FRAP), ABTS, superoxide anion and hydroxyl radical scavenging tests.
26616946	5	32	from	activities	729:738	arg1	radical					821:827	hydroxyl radical	812:827	hydroxyl radical	812:827	The FDPEPS showed the highest antioxidant activities in ferric reducing antioxidant power (FRAP), ABTS, superoxide anion and hydroxyl radical scavenging tests.
26616946	5	32	from	activities	729:738	arg1	power					771:775	ferric reducing antioxidant power	743:775	ferric reducing antioxidant power (FRAP)	743:782	The FDPEPS showed the highest antioxidant activities in ferric reducing antioxidant power (FRAP), ABTS, superoxide anion and hydroxyl radical scavenging tests.
26616946	5	32	from	activities	729:738	arg1	FRAP					778:781	FRAP	778:781	FRAP	778:781	The FDPEPS showed the highest antioxidant activities in ferric reducing antioxidant power (FRAP), ABTS, superoxide anion and hydroxyl radical scavenging tests.
26616946	5	32	from	activities	729:738	arg1	ABTS					785:788	ABTS	785:788	ABTS	785:788	The FDPEPS showed the highest antioxidant activities in ferric reducing antioxidant power (FRAP), ABTS, superoxide anion and hydroxyl radical scavenging tests.
26616946	5	32	from	activities	729:738	arg1	anion					802:806	superoxide anion	791:806	superoxide anion	791:806	The FDPEPS showed the highest antioxidant activities in ferric reducing antioxidant power (FRAP), ABTS, superoxide anion and hydroxyl radical scavenging tests.
26616946	3	33	theme	performance	407:417	arg1	HPLC					442:445	HPLC	442:445	HPLC	442:445	High performance liquid chromatography (HPLC) identified that FDPEPS, ODPEPS, and BTPEPS were composed of mannose (5.75%, 5.52%, 6.97%), glucose (88.90%, 89.31%, 87.68%) and galactose (5.34%, 5.17%, 5.35%).
26616946	3	33	theme	performance	407:417	arg1	chromatography					426:439	High performance liquid chromatography	402:439	High performance liquid chromatography (HPLC)	402:446	High performance liquid chromatography (HPLC) identified that FDPEPS, ODPEPS, and BTPEPS were composed of mannose (5.75%, 5.52%, 6.97%), glucose (88.90%, 89.31%, 87.68%) and galactose (5.34%, 5.17%, 5.35%).
26616946	1	34	dep	Pleurotus	204:212	arg1	ODPEPS					231:236	ODPEPS	231:236	ODPEPS	231:236	Polysaccharides were extracted from freeze-dried-, oven-dried- and boiling-treated Pleurotus eryngii (FDPEPS, ODPEPS and BTPEPS).
26616946	1	34	dep	Pleurotus	204:212	arg1	FDPEPS					223:228	FDPEPS	223:228	FDPEPS	223:228	Polysaccharides were extracted from freeze-dried-, oven-dried- and boiling-treated Pleurotus eryngii (FDPEPS, ODPEPS and BTPEPS).
26616946	1	34	dep	Pleurotus	204:212	arg1	eryngii					214:220	Pleurotus eryngii (FDPEPS, ODPEPS and BTPEPS)	204:248	Pleurotus eryngii (FDPEPS, ODPEPS and BTPEPS)	204:248	Polysaccharides were extracted from freeze-dried-, oven-dried- and boiling-treated Pleurotus eryngii (FDPEPS, ODPEPS and BTPEPS).
26616946	1	34	dep	Pleurotus	204:212	arg1	BTPEPS					242:247	BTPEPS	242:247	BTPEPS	242:247	Polysaccharides were extracted from freeze-dried-, oven-dried- and boiling-treated Pleurotus eryngii (FDPEPS, ODPEPS and BTPEPS).
26616946	3	35	theme	liquid	419:424	arg1	HPLC					442:445	HPLC	442:445	HPLC	442:445	High performance liquid chromatography (HPLC) identified that FDPEPS, ODPEPS, and BTPEPS were composed of mannose (5.75%, 5.52%, 6.97%), glucose (88.90%, 89.31%, 87.68%) and galactose (5.34%, 5.17%, 5.35%).
26616946	3	35	theme	liquid	419:424	arg1	chromatography					426:439	High performance liquid chromatography	402:439	High performance liquid chromatography (HPLC)	402:446	High performance liquid chromatography (HPLC) identified that FDPEPS, ODPEPS, and BTPEPS were composed of mannose (5.75%, 5.52%, 6.97%), glucose (88.90%, 89.31%, 87.68%) and galactose (5.34%, 5.17%, 5.35%).
26616946	2	36	theme	protein	350:356	arg1	content					358:364	protein content	350:364	protein content	350:364	Yield of FDPEPS and its total carbohydrates, total polysaccharides, reducing sugars, phenolics and protein content were higher than ODPEPS and BTPEPS.
26616946	2	37	theme	total	275:279	arg1	carbohydrates					281:293	its total carbohydrates	271:293	its total carbohydrates	271:293	Yield of FDPEPS and its total carbohydrates, total polysaccharides, reducing sugars, phenolics and protein content were higher than ODPEPS and BTPEPS.
26616946	5	38	theme	superoxide	791:800	arg1	anion					802:806	superoxide anion	791:806	superoxide anion	791:806	The FDPEPS showed the highest antioxidant activities in ferric reducing antioxidant power (FRAP), ABTS, superoxide anion and hydroxyl radical scavenging tests.
26616946	0	39	dep	Pleurotus	80:88	arg1	eryngii					90:96	Pleurotus eryngii	80:96	Pleurotus eryngii	80:96	Characterization, antioxidative and bifidogenic effects of polysaccharides from Pleurotus eryngii after heat treatments.
26616946	6	40	theme	confocal	905:912	arg1	micrographs					914:924	confocal micrographs	905:924	confocal micrographs of soymilk added with ODPEPS	905:953	Denser aggregates and larger serum pores were observed in confocal micrographs of soymilk added with ODPEPS.
26616946	4	41	theme	saccharic	650:658	arg1	bands					671:675	typical saccharic absorption bands	642:675	typical saccharic absorption bands	642:675	All three polysaccharides showed typical saccharic absorption bands in FT-IR.
26616946	8	42	theme	FDPEPS	1222:1227	arg1	microstructures					1203:1217	microstructures	1203:1217	microstructures	1203:1217	Heat treatments applied before extraction affected the properties, composition and microstructures of FDPEPS, ODPEPS and BTPEPS.
26616946	8	42	theme	FDPEPS	1222:1227	arg1	composition					1187:1197	composition	1187:1197	composition	1187:1197	Heat treatments applied before extraction affected the properties, composition and microstructures of FDPEPS, ODPEPS and BTPEPS.
26616946	8	42	theme	FDPEPS	1222:1227	arg1	properties					1175:1184	the properties	1171:1184	the properties	1171:1184	Heat treatments applied before extraction affected the properties, composition and microstructures of FDPEPS, ODPEPS and BTPEPS.
26616946	5	43	theme	reducing	750:757	arg1	FRAP					778:781	FRAP	778:781	FRAP	778:781	The FDPEPS showed the highest antioxidant activities in ferric reducing antioxidant power (FRAP), ABTS, superoxide anion and hydroxyl radical scavenging tests.
26616946	5	43	theme	reducing	750:757	arg1	power					771:775	ferric reducing antioxidant power	743:775	ferric reducing antioxidant power (FRAP)	743:782	The FDPEPS showed the highest antioxidant activities in ferric reducing antioxidant power (FRAP), ABTS, superoxide anion and hydroxyl radical scavenging tests.
26616946	0	44	theme	polysaccharides	59:73	arg1	effects					48:54	antioxidative and bifidogenic effects	18:54	antioxidative and bifidogenic effects of polysaccharides from Pleurotus eryngii after heat treatments	18:118	Characterization, antioxidative and bifidogenic effects of polysaccharides from Pleurotus eryngii after heat treatments.
26616946	0	44	theme	polysaccharides	59:73	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization, antioxidative and bifidogenic effects of polysaccharides from Pleurotus eryngii after heat treatments.
26616946	2	45	theme	FDPEPS	260:265	arg1	Yield					251:255	Yield	251:255	Yield of FDPEPS and its total carbohydrates, total polysaccharides, reducing sugars, phenolics and protein content	251:364	Yield of FDPEPS and its total carbohydrates, total polysaccharides, reducing sugars, phenolics and protein content were higher than ODPEPS and BTPEPS.
26616946	8	46	theme	ODPEPS	1230:1235	arg1	microstructures					1203:1217	microstructures	1203:1217	microstructures	1203:1217	Heat treatments applied before extraction affected the properties, composition and microstructures of FDPEPS, ODPEPS and BTPEPS.
26616946	8	46	theme	ODPEPS	1230:1235	arg1	composition					1187:1197	composition	1187:1197	composition	1187:1197	Heat treatments applied before extraction affected the properties, composition and microstructures of FDPEPS, ODPEPS and BTPEPS.
26616946	8	46	theme	ODPEPS	1230:1235	arg1	properties					1175:1184	the properties	1171:1184	the properties	1171:1184	Heat treatments applied before extraction affected the properties, composition and microstructures of FDPEPS, ODPEPS and BTPEPS.
26457879	5	0	with	consistent	874:883	arg1	observation					894:904	the observation that the components of the dispersion were in intimate contact over a 10-20 nm length scale	890:996	the observation that the components of the dispersion were in intimate contact over a 10-20 nm length scale	890:996	Proton spin-lattice relaxation times in the laboratory and rotating frames ((1)H T1 and T1ρ), as measured by SSNMR, were consistent with the observation that the components of the dispersion were in intimate contact over a 10-20 nm length scale.
26457879	7	1	theme	phase	1313:1317	arg1	separation					1319:1328	phase separation	1313:1328	phase separation at 25 °C	1313:1337	The free energy of mixing was found to be positive for all ASDs, indicating that the drug and polymer are thermodynamically predisposed to phase separation at 25 °C.
26457879	5	2	from	times	784:788	arg1	laboratory					797:806	laboratory	797:806	laboratory	797:806	Proton spin-lattice relaxation times in the laboratory and rotating frames ((1)H T1 and T1ρ), as measured by SSNMR, were consistent with the observation that the components of the dispersion were in intimate contact over a 10-20 nm length scale.
26457879	5	2	from	times	784:788	arg1	frames					821:826	rotating frames	812:826	rotating frames	812:826	Proton spin-lattice relaxation times in the laboratory and rotating frames ((1)H T1 and T1ρ), as measured by SSNMR, were consistent with the observation that the components of the dispersion were in intimate contact over a 10-20 nm length scale.
26457879	5	3	theme	10-20	976:980	arg1	nm					982:983	nm	982:983	nm	982:983	Proton spin-lattice relaxation times in the laboratory and rotating frames ((1)H T1 and T1ρ), as measured by SSNMR, were consistent with the observation that the components of the dispersion were in intimate contact over a 10-20 nm length scale.
26457879	2	4	theme	solid-state	427:437	arg1	SSNMR					444:448	SSNMR	444:448	SSNMR	444:448	In this article, spray dried amorphous dispersions (ASDs) of AMG 517 and HPMC-as were studied by differential scanning calorimetry (DSC), solid-state NMR (SSNMR), and solution calorimetry.
26457879	2	4	theme	solid-state	427:437	arg1	NMR					439:441	solid-state NMR	427:441	solid-state NMR (SSNMR)	427:449	In this article, spray dried amorphous dispersions (ASDs) of AMG 517 and HPMC-as were studied by differential scanning calorimetry (DSC), solid-state NMR (SSNMR), and solution calorimetry.
26457879	6	5	theme	mixing	1151:1156	arg1	energy					1141:1146	the free energy	1132:1146	the free energy of mixing	1132:1156	Based on the heat of mixing calculated from solution calorimetry and the entropy of mixing calculated from the Flory-Huggins theory, the free energy of mixing was calculated.
26457879	1	6	theme	Intimate	137:144	arg1	mixing					152:157	Intimate phase mixing	137:157	Intimate phase mixing between the drug and the polymer	137:190	Intimate phase mixing between the drug and the polymer is considered a prerequisite to achieve good physical stability for amorphous solid dispersions.
26457879	2	7	theme	solution	456:463	arg1	calorimetry					465:475	solution calorimetry	456:475	solution calorimetry	456:475	In this article, spray dried amorphous dispersions (ASDs) of AMG 517 and HPMC-as were studied by differential scanning calorimetry (DSC), solid-state NMR (SSNMR), and solution calorimetry.
26457879	0	8	from	Investigation	0:12	arg1	Dispersions					49:59	Amorphous Solid Dispersions	33:59	Amorphous Solid Dispersions of AMG 517 in HPMC-AS Using DSC, Solid-State NMR, and Solution Calorimetry	33:134	Investigation of Phase Mixing in Amorphous Solid Dispersions of AMG 517 in HPMC-AS Using DSC, Solid-State NMR, and Solution Calorimetry.
26457879	3	9	theme	DSC	478:480	arg1	analysis					482:489	DSC analysis	478:489	DSC analysis	478:489	DSC analysis showed a weakly asymmetric (ΔTg ≈ 13.5) system with a single glass transition for blends of different compositions indicating phase mixing.
26457879	1	10	theme	good	232:235	arg1	stability					246:254	good physical stability	232:254	good physical stability for amorphous solid dispersions	232:286	Intimate phase mixing between the drug and the polymer is considered a prerequisite to achieve good physical stability for amorphous solid dispersions.
26457879	0	11	from	Dispersions	49:59	arg1	HPMC-AS					75:81	HPMC-AS	75:81	HPMC-AS Using DSC, Solid-State NMR, and Solution Calorimetry	75:134	Investigation of Phase Mixing in Amorphous Solid Dispersions of AMG 517 in HPMC-AS Using DSC, Solid-State NMR, and Solution Calorimetry.
26457879	1	12	theme	physical	237:244	arg1	stability					246:254	good physical stability	232:254	good physical stability for amorphous solid dispersions	232:286	Intimate phase mixing between the drug and the polymer is considered a prerequisite to achieve good physical stability for amorphous solid dispersions.
26457879	4	13	theme	Tg-composition	635:648	arg1	data					650:653	The Tg-composition data	631:653	The Tg-composition data	631:653	The Tg-composition data was modeled using the BKCV equation to accommodate the observed negative deviation from ideality.
26457879	6	14	theme	free	1136:1139	arg1	energy					1141:1146	the free energy	1132:1146	the free energy of mixing	1132:1156	Based on the heat of mixing calculated from solution calorimetry and the entropy of mixing calculated from the Flory-Huggins theory, the free energy of mixing was calculated.
26457879	7	15	theme	free	1178:1181	arg1	positive					1216:1223	positive	1216:1223	positive	1216:1223	The free energy of mixing was found to be positive for all ASDs, indicating that the drug and polymer are thermodynamically predisposed to phase separation at 25 °C.
26457879	7	15	theme	free	1178:1181	arg1	energy					1183:1188	The free energy	1174:1188	The free energy of mixing	1174:1198	The free energy of mixing was found to be positive for all ASDs, indicating that the drug and polymer are thermodynamically predisposed to phase separation at 25 °C.
26457879	6	16	theme	solution	1043:1050	arg1	calorimetry					1052:1062	solution calorimetry	1043:1062	solution calorimetry	1043:1062	Based on the heat of mixing calculated from solution calorimetry and the entropy of mixing calculated from the Flory-Huggins theory, the free energy of mixing was calculated.
26457879	5	17	theme	rotating	812:819	arg1	frames					821:826	rotating frames	812:826	rotating frames	812:826	Proton spin-lattice relaxation times in the laboratory and rotating frames ((1)H T1 and T1ρ), as measured by SSNMR, were consistent with the observation that the components of the dispersion were in intimate contact over a 10-20 nm length scale.
26457879	1	18	theme	phase	146:150	arg1	mixing					152:157	Intimate phase mixing	137:157	Intimate phase mixing between the drug and the polymer	137:190	Intimate phase mixing between the drug and the polymer is considered a prerequisite to achieve good physical stability for amorphous solid dispersions.
26457879	5	19	theme	length	985:990	arg1	scale					992:996	a 10-20 nm length scale	974:996	a 10-20 nm length scale	974:996	Proton spin-lattice relaxation times in the laboratory and rotating frames ((1)H T1 and T1ρ), as measured by SSNMR, were consistent with the observation that the components of the dispersion were in intimate contact over a 10-20 nm length scale.
26457879	9	20	theme	kinetic	1517:1523	arg1	mixing					1531:1536	This kinetic phase mixing	1512:1536	This kinetic phase mixing	1512:1536	This kinetic phase mixing is responsible for the physical stability of the ASD.
26457879	5	21	dep	T1	834:835	arg1	1					830:830	1	830:830	1	830:830	Proton spin-lattice relaxation times in the laboratory and rotating frames ((1)H T1 and T1ρ), as measured by SSNMR, were consistent with the observation that the components of the dispersion were in intimate contact over a 10-20 nm length scale.
26457879	2	22	theme	AMG	350:352	arg1	dispersions					328:338	spray dried amorphous dispersions	306:338	spray dried amorphous dispersions (ASDs) of AMG 517 and HPMC-as	306:368	In this article, spray dried amorphous dispersions (ASDs) of AMG 517 and HPMC-as were studied by differential scanning calorimetry (DSC), solid-state NMR (SSNMR), and solution calorimetry.
26457879	2	22	theme	AMG	350:352	arg1	ASDs					341:344	ASDs	341:344	ASDs	341:344	In this article, spray dried amorphous dispersions (ASDs) of AMG 517 and HPMC-as were studied by differential scanning calorimetry (DSC), solid-state NMR (SSNMR), and solution calorimetry.
26457879	9	23	theme	phase	1525:1529	arg1	mixing					1531:1536	This kinetic phase mixing	1512:1536	This kinetic phase mixing	1512:1536	This kinetic phase mixing is responsible for the physical stability of the ASD.
26457879	9	24	theme	ASD	1587:1589	arg1	stability					1570:1578	the physical stability	1557:1578	the physical stability of the ASD	1557:1589	This kinetic phase mixing is responsible for the physical stability of the ASD.
26457879	5	25	theme	nm	982:983	arg1	scale					992:996	a 10-20 nm length scale	974:996	a 10-20 nm length scale	974:996	Proton spin-lattice relaxation times in the laboratory and rotating frames ((1)H T1 and T1ρ), as measured by SSNMR, were consistent with the observation that the components of the dispersion were in intimate contact over a 10-20 nm length scale.
26457879	3	26	theme	phase	617:621	arg1	mixing					623:628	phase mixing	617:628	phase mixing	617:628	DSC analysis showed a weakly asymmetric (ΔTg ≈ 13.5) system with a single glass transition for blends of different compositions indicating phase mixing.
26457879	2	27	theme	scanning	399:406	arg1	DSC					421:423	DSC	421:423	DSC	421:423	In this article, spray dried amorphous dispersions (ASDs) of AMG 517 and HPMC-as were studied by differential scanning calorimetry (DSC), solid-state NMR (SSNMR), and solution calorimetry.
26457879	2	27	theme	scanning	399:406	arg1	calorimetry					408:418	differential scanning calorimetry	386:418	differential scanning calorimetry (DSC)	386:424	In this article, spray dried amorphous dispersions (ASDs) of AMG 517 and HPMC-as were studied by differential scanning calorimetry (DSC), solid-state NMR (SSNMR), and solution calorimetry.
26457879	3	28	theme	asymmetric	507:516	arg1	system					531:536	a weakly asymmetric (ΔTg ≈ 13.5) system	498:536	a weakly asymmetric (ΔTg ≈ 13.5) system with a single glass transition for blends of different compositions	498:604	DSC analysis showed a weakly asymmetric (ΔTg ≈ 13.5) system with a single glass transition for blends of different compositions indicating phase mixing.
26457879	0	29	theme	Mixing	23:28	arg1	Investigation					0:12	Investigation	0:12	Investigation of Phase Mixing in Amorphous Solid Dispersions of AMG 517 in HPMC-AS Using DSC, Solid-State NMR, and Solution Calorimetry.	0:135	Investigation of Phase Mixing in Amorphous Solid Dispersions of AMG 517 in HPMC-AS Using DSC, Solid-State NMR, and Solution Calorimetry.
26457879	4	30	theme	BKCV	677:680	arg1	equation					682:689	the BKCV equation	673:689	the BKCV equation	673:689	The Tg-composition data was modeled using the BKCV equation to accommodate the observed negative deviation from ideality.
26457879	5	31	theme	relaxation	773:782	arg1	times					784:788	Proton spin-lattice relaxation times	753:788	Proton spin-lattice relaxation times	753:788	Proton spin-lattice relaxation times in the laboratory and rotating frames ((1)H T1 and T1ρ), as measured by SSNMR, were consistent with the observation that the components of the dispersion were in intimate contact over a 10-20 nm length scale.
26457879	0	32	theme	Phase	17:21	arg1	Mixing					23:28	Phase Mixing	17:28	Phase Mixing	17:28	Investigation of Phase Mixing in Amorphous Solid Dispersions of AMG 517 in HPMC-AS Using DSC, Solid-State NMR, and Solution Calorimetry.
26457879	1	33	theme	amorphous	260:268	arg1	dispersions					276:286	amorphous solid dispersions	260:286	amorphous solid dispersions	260:286	Intimate phase mixing between the drug and the polymer is considered a prerequisite to achieve good physical stability for amorphous solid dispersions.
26457879	0	34	theme	Solid-State	94:104	arg1	NMR					106:108	Solid-State NMR	94:108	Solid-State NMR	94:108	Investigation of Phase Mixing in Amorphous Solid Dispersions of AMG 517 in HPMC-AS Using DSC, Solid-State NMR, and Solution Calorimetry.
26457879	5	35	theme	intimate	952:959	arg1	contact					961:967	intimate contact	952:967	intimate contact	952:967	Proton spin-lattice relaxation times in the laboratory and rotating frames ((1)H T1 and T1ρ), as measured by SSNMR, were consistent with the observation that the components of the dispersion were in intimate contact over a 10-20 nm length scale.
26457879	2	36	theme	amorphous	318:326	arg1	dispersions					328:338	spray dried amorphous dispersions	306:338	spray dried amorphous dispersions (ASDs) of AMG 517 and HPMC-as	306:368	In this article, spray dried amorphous dispersions (ASDs) of AMG 517 and HPMC-as were studied by differential scanning calorimetry (DSC), solid-state NMR (SSNMR), and solution calorimetry.
26457879	2	36	theme	amorphous	318:326	arg1	ASDs					341:344	ASDs	341:344	ASDs	341:344	In this article, spray dried amorphous dispersions (ASDs) of AMG 517 and HPMC-as were studied by differential scanning calorimetry (DSC), solid-state NMR (SSNMR), and solution calorimetry.
26457879	3	37	dep	asymmetric	507:516	arg1	≈					523:523	ΔTg ≈ 13.5	519:528	ΔTg ≈ 13.5	519:528	DSC analysis showed a weakly asymmetric (ΔTg ≈ 13.5) system with a single glass transition for blends of different compositions indicating phase mixing.
26457879	6	38	theme	mixing	1020:1025	arg1	heat					1012:1015	the heat	1008:1015	the heat of mixing calculated from solution calorimetry	1008:1062	Based on the heat of mixing calculated from solution calorimetry and the entropy of mixing calculated from the Flory-Huggins theory, the free energy of mixing was calculated.
26457879	6	38	theme	mixing	1020:1025	arg1	entropy					1072:1078	the entropy	1068:1078	the entropy of mixing calculated from the Flory-Huggins theory	1068:1129	Based on the heat of mixing calculated from solution calorimetry and the entropy of mixing calculated from the Flory-Huggins theory, the free energy of mixing was calculated.
26457879	0	39	theme	Amorphous	33:41	arg1	Dispersions					49:59	Amorphous Solid Dispersions	33:59	Amorphous Solid Dispersions of AMG 517 in HPMC-AS Using DSC, Solid-State NMR, and Solution Calorimetry	33:134	Investigation of Phase Mixing in Amorphous Solid Dispersions of AMG 517 in HPMC-AS Using DSC, Solid-State NMR, and Solution Calorimetry.
26457879	1	40	theme	solid	270:274	arg1	dispersions					276:286	amorphous solid dispersions	260:286	amorphous solid dispersions	260:286	Intimate phase mixing between the drug and the polymer is considered a prerequisite to achieve good physical stability for amorphous solid dispersions.
26457879	6	41	theme	Flory-Huggins	1110:1122	arg1	theory					1124:1129	the Flory-Huggins theory	1106:1129	the Flory-Huggins theory	1106:1129	Based on the heat of mixing calculated from solution calorimetry and the entropy of mixing calculated from the Flory-Huggins theory, the free energy of mixing was calculated.
26457879	2	42	theme	HPMC-as	362:368	arg1	dispersions					328:338	spray dried amorphous dispersions	306:338	spray dried amorphous dispersions (ASDs) of AMG 517 and HPMC-as	306:368	In this article, spray dried amorphous dispersions (ASDs) of AMG 517 and HPMC-as were studied by differential scanning calorimetry (DSC), solid-state NMR (SSNMR), and solution calorimetry.
26457879	2	42	theme	HPMC-as	362:368	arg1	ASDs					341:344	ASDs	341:344	ASDs	341:344	In this article, spray dried amorphous dispersions (ASDs) of AMG 517 and HPMC-as were studied by differential scanning calorimetry (DSC), solid-state NMR (SSNMR), and solution calorimetry.
26457879	7	43	theme	mixing	1193:1198	arg1	positive					1216:1223	positive	1216:1223	positive	1216:1223	The free energy of mixing was found to be positive for all ASDs, indicating that the drug and polymer are thermodynamically predisposed to phase separation at 25 °C.
26457879	7	43	theme	mixing	1193:1198	arg1	energy					1183:1188	The free energy	1174:1188	The free energy of mixing	1174:1198	The free energy of mixing was found to be positive for all ASDs, indicating that the drug and polymer are thermodynamically predisposed to phase separation at 25 °C.
26457879	3	44	theme	single	545:550	arg1	transition					558:567	a single glass transition	543:567	a single glass transition	543:567	DSC analysis showed a weakly asymmetric (ΔTg ≈ 13.5) system with a single glass transition for blends of different compositions indicating phase mixing.
26457879	2	45	theme	differential	386:397	arg1	DSC					421:423	DSC	421:423	DSC	421:423	In this article, spray dried amorphous dispersions (ASDs) of AMG 517 and HPMC-as were studied by differential scanning calorimetry (DSC), solid-state NMR (SSNMR), and solution calorimetry.
26457879	2	45	theme	differential	386:397	arg1	calorimetry					408:418	differential scanning calorimetry	386:418	differential scanning calorimetry (DSC)	386:424	In this article, spray dried amorphous dispersions (ASDs) of AMG 517 and HPMC-as were studied by differential scanning calorimetry (DSC), solid-state NMR (SSNMR), and solution calorimetry.
26457879	5	46	theme	Proton	753:758	arg1	times					784:788	Proton spin-lattice relaxation times	753:788	Proton spin-lattice relaxation times	753:788	Proton spin-lattice relaxation times in the laboratory and rotating frames ((1)H T1 and T1ρ), as measured by SSNMR, were consistent with the observation that the components of the dispersion were in intimate contact over a 10-20 nm length scale.
26457879	7	47	from	°C	1336:1337	arg1	separation					1319:1328	phase separation	1313:1328	phase separation at 25 °C	1313:1337	The free energy of mixing was found to be positive for all ASDs, indicating that the drug and polymer are thermodynamically predisposed to phase separation at 25 °C.
26457879	9	48	theme	physical	1561:1568	arg1	stability					1570:1578	the physical stability	1557:1578	the physical stability of the ASD	1557:1589	This kinetic phase mixing is responsible for the physical stability of the ASD.
26457879	3	49	theme	glass	552:556	arg1	transition					558:567	a single glass transition	543:567	a single glass transition	543:567	DSC analysis showed a weakly asymmetric (ΔTg ≈ 13.5) system with a single glass transition for blends of different compositions indicating phase mixing.
26457879	5	50	dep	laboratory	797:806	arg1	T1					834:835	(1)H T1	829:835	(1)H T1	829:835	Proton spin-lattice relaxation times in the laboratory and rotating frames ((1)H T1 and T1ρ), as measured by SSNMR, were consistent with the observation that the components of the dispersion were in intimate contact over a 10-20 nm length scale.
26457879	5	50	dep	laboratory	797:806	arg1	the					793:795	the	793:795	the	793:795	Proton spin-lattice relaxation times in the laboratory and rotating frames ((1)H T1 and T1ρ), as measured by SSNMR, were consistent with the observation that the components of the dispersion were in intimate contact over a 10-20 nm length scale.
26457879	5	50	dep	laboratory	797:806	arg1	T1ρ					841:843	T1ρ	841:843	T1ρ	841:843	Proton spin-lattice relaxation times in the laboratory and rotating frames ((1)H T1 and T1ρ), as measured by SSNMR, were consistent with the observation that the components of the dispersion were in intimate contact over a 10-20 nm length scale.
26457879	2	51	theme	dried	312:316	arg1	dispersions					328:338	spray dried amorphous dispersions	306:338	spray dried amorphous dispersions (ASDs) of AMG 517 and HPMC-as	306:368	In this article, spray dried amorphous dispersions (ASDs) of AMG 517 and HPMC-as were studied by differential scanning calorimetry (DSC), solid-state NMR (SSNMR), and solution calorimetry.
26457879	2	51	theme	dried	312:316	arg1	ASDs					341:344	ASDs	341:344	ASDs	341:344	In this article, spray dried amorphous dispersions (ASDs) of AMG 517 and HPMC-as were studied by differential scanning calorimetry (DSC), solid-state NMR (SSNMR), and solution calorimetry.
26457879	0	52	theme	Solid	43:47	arg1	Dispersions					49:59	Amorphous Solid Dispersions	33:59	Amorphous Solid Dispersions of AMG 517 in HPMC-AS Using DSC, Solid-State NMR, and Solution Calorimetry	33:134	Investigation of Phase Mixing in Amorphous Solid Dispersions of AMG 517 in HPMC-AS Using DSC, Solid-State NMR, and Solution Calorimetry.
26457879	0	53	theme	Solution	115:122	arg1	Calorimetry					124:134	Solution Calorimetry	115:134	Solution Calorimetry	115:134	Investigation of Phase Mixing in Amorphous Solid Dispersions of AMG 517 in HPMC-AS Using DSC, Solid-State NMR, and Solution Calorimetry.
26457879	3	54	theme	different	583:591	arg1	compositions					593:604	different compositions	583:604	different compositions	583:604	DSC analysis showed a weakly asymmetric (ΔTg ≈ 13.5) system with a single glass transition for blends of different compositions indicating phase mixing.
26457879	3	55	theme	ΔTg	519:521	arg1	≈					523:523	ΔTg ≈ 13.5	519:528	ΔTg ≈ 13.5	519:528	DSC analysis showed a weakly asymmetric (ΔTg ≈ 13.5) system with a single glass transition for blends of different compositions indicating phase mixing.
26457879	2	56	theme	spray	306:310	arg1	dispersions					328:338	spray dried amorphous dispersions	306:338	spray dried amorphous dispersions (ASDs) of AMG 517 and HPMC-as	306:368	In this article, spray dried amorphous dispersions (ASDs) of AMG 517 and HPMC-as were studied by differential scanning calorimetry (DSC), solid-state NMR (SSNMR), and solution calorimetry.
26457879	2	56	theme	spray	306:310	arg1	ASDs					341:344	ASDs	341:344	ASDs	341:344	In this article, spray dried amorphous dispersions (ASDs) of AMG 517 and HPMC-as were studied by differential scanning calorimetry (DSC), solid-state NMR (SSNMR), and solution calorimetry.
26457879	8	57	theme	drying	1496:1501	arg1	process					1503:1509	the spray drying process	1486:1509	the spray drying process	1486:1509	This suggests that miscibility measured by DSC and SSNMR is achieved kinetically as the result of intimate mixing between drug and polymer during the spray drying process.
26457879	5	58	dep	times	784:788	arg1	measured					850:857	measured	850:857	measured by SSNMR	850:866	Proton spin-lattice relaxation times in the laboratory and rotating frames ((1)H T1 and T1ρ), as measured by SSNMR, were consistent with the observation that the components of the dispersion were in intimate contact over a 10-20 nm length scale.
26457879	3	59	theme	compositions	593:604	arg1	blends					573:578	blends	573:578	blends of different compositions	573:604	DSC analysis showed a weakly asymmetric (ΔTg ≈ 13.5) system with a single glass transition for blends of different compositions indicating phase mixing.
26457879	4	60	theme	negative	719:726	arg1	deviation					728:736	the observed negative deviation	706:736	the observed negative deviation from ideality	706:750	The Tg-composition data was modeled using the BKCV equation to accommodate the observed negative deviation from ideality.
26457879	4	61	from	ideality	743:750	arg1	deviation					728:736	the observed negative deviation	706:736	the observed negative deviation from ideality	706:750	The Tg-composition data was modeled using the BKCV equation to accommodate the observed negative deviation from ideality.
26457879	8	62	theme	spray	1490:1494	arg1	process					1503:1509	the spray drying process	1486:1509	the spray drying process	1486:1509	This suggests that miscibility measured by DSC and SSNMR is achieved kinetically as the result of intimate mixing between drug and polymer during the spray drying process.
26457879	8	63	theme	mixing	1447:1452	arg1	miscibility					1359:1369	miscibility	1359:1369	miscibility measured by DSC and SSNMR	1359:1395	This suggests that miscibility measured by DSC and SSNMR is achieved kinetically as the result of intimate mixing between drug and polymer during the spray drying process.
26457879	8	63	theme	mixing	1447:1452	arg1	result					1428:1433	the result	1424:1433	the result of intimate mixing between drug and polymer	1424:1477	This suggests that miscibility measured by DSC and SSNMR is achieved kinetically as the result of intimate mixing between drug and polymer during the spray drying process.
26457879	5	64	theme	dispersion	933:942	arg1	components					915:924	the components	911:924	the components of the dispersion	911:942	Proton spin-lattice relaxation times in the laboratory and rotating frames ((1)H T1 and T1ρ), as measured by SSNMR, were consistent with the observation that the components of the dispersion were in intimate contact over a 10-20 nm length scale.
26457879	5	65	theme	H	832:832	arg1	T1					834:835	(1)H T1	829:835	(1)H T1	829:835	Proton spin-lattice relaxation times in the laboratory and rotating frames ((1)H T1 and T1ρ), as measured by SSNMR, were consistent with the observation that the components of the dispersion were in intimate contact over a 10-20 nm length scale.
26457879	4	66	theme	observed	710:717	arg1	deviation					728:736	the observed negative deviation	706:736	the observed negative deviation from ideality	706:750	The Tg-composition data was modeled using the BKCV equation to accommodate the observed negative deviation from ideality.
26457879	8	67	theme	intimate	1438:1445	arg1	mixing					1447:1452	intimate mixing	1438:1452	intimate mixing between drug and polymer	1438:1477	This suggests that miscibility measured by DSC and SSNMR is achieved kinetically as the result of intimate mixing between drug and polymer during the spray drying process.
26457879	0	68	theme	AMG	64:66	arg1	Dispersions					49:59	Amorphous Solid Dispersions	33:59	Amorphous Solid Dispersions of AMG 517 in HPMC-AS Using DSC, Solid-State NMR, and Solution Calorimetry	33:134	Investigation of Phase Mixing in Amorphous Solid Dispersions of AMG 517 in HPMC-AS Using DSC, Solid-State NMR, and Solution Calorimetry.
26457879	3	69	with	system	531:536	arg1	transition					558:567	a single glass transition	543:567	a single glass transition	543:567	DSC analysis showed a weakly asymmetric (ΔTg ≈ 13.5) system with a single glass transition for blends of different compositions indicating phase mixing.
26457879	5	70	theme	spin-lattice	760:771	arg1	times					784:788	Proton spin-lattice relaxation times	753:788	Proton spin-lattice relaxation times	753:788	Proton spin-lattice relaxation times in the laboratory and rotating frames ((1)H T1 and T1ρ), as measured by SSNMR, were consistent with the observation that the components of the dispersion were in intimate contact over a 10-20 nm length scale.
26457879	6	71	theme	mixing	1083:1088	arg1	heat					1012:1015	the heat	1008:1015	the heat of mixing calculated from solution calorimetry	1008:1062	Based on the heat of mixing calculated from solution calorimetry and the entropy of mixing calculated from the Flory-Huggins theory, the free energy of mixing was calculated.
26457879	6	71	theme	mixing	1083:1088	arg1	entropy					1072:1078	the entropy	1068:1078	the entropy of mixing calculated from the Flory-Huggins theory	1068:1129	Based on the heat of mixing calculated from solution calorimetry and the entropy of mixing calculated from the Flory-Huggins theory, the free energy of mixing was calculated.
29433252	1	0	theme	cheeses	255:261	arg1	60days					230:235	60days	230:235	60days	230:235	This study aimed at determining the composition and rheology changes during the ripening (60days) of Manchego-type cheeses prepared with Lactococcus lactis subsp.
29433252	1	0	theme	cheeses	255:261	arg1	ripening					220:227	the ripening	216:227	the ripening (60days) of Manchego-type cheeses prepared with Lactococcus lactis subsp	216:300	This study aimed at determining the composition and rheology changes during the ripening (60days) of Manchego-type cheeses prepared with Lactococcus lactis subsp.
29433252	0	1	theme	rheological	75:85	arg1	properties					87:96	rheological properties	75:96	rheological properties	75:96	Effect of free and microencapsulated Lactococcus lactis on composition and rheological properties of Manchego-type cheeses during ripening.
29433252	4	2	theme	ripening	691:698	arg1	beginning					666:674	the beginning	662:674	the beginning	662:674	The composition indicated that microencapsulation had a greater influence than the period of ripening since discrepancies were statistically significant (p<0.05) regarding the levels of protein, moisture, total solids and pH between the two cheeses both at the beginning and end of the ripening.
29433252	4	2	theme	ripening	691:698	arg1	end					680:682	end	680:682	end	680:682	The composition indicated that microencapsulation had a greater influence than the period of ripening since discrepancies were statistically significant (p<0.05) regarding the levels of protein, moisture, total solids and pH between the two cheeses both at the beginning and end of the ripening.
29433252	4	3	theme	moisture	600:607	arg1	levels					581:586	the levels	577:586	the levels of protein, moisture, total solids and pH	577:628	The composition indicated that microencapsulation had a greater influence than the period of ripening since discrepancies were statistically significant (p<0.05) regarding the levels of protein, moisture, total solids and pH between the two cheeses both at the beginning and end of the ripening.
29433252	0	4	theme	Manchego-type	101:113	arg1	composition					59:69	composition	59:69	composition	59:69	Effect of free and microencapsulated Lactococcus lactis on composition and rheological properties of Manchego-type cheeses during ripening.
29433252	0	4	theme	Manchego-type	101:113	arg1	properties					87:96	rheological properties	75:96	rheological properties	75:96	Effect of free and microencapsulated Lactococcus lactis on composition and rheological properties of Manchego-type cheeses during ripening.
29433252	0	5	from	Effect	0:5	arg1	composition					59:69	composition	59:69	composition	59:69	Effect of free and microencapsulated Lactococcus lactis on composition and rheological properties of Manchego-type cheeses during ripening.
29433252	0	5	from	Effect	0:5	arg1	properties					87:96	rheological properties	75:96	rheological properties	75:96	Effect of free and microencapsulated Lactococcus lactis on composition and rheological properties of Manchego-type cheeses during ripening.
29433252	4	6	theme	solids	616:621	arg1	levels					581:586	the levels	577:586	the levels of protein, moisture, total solids and pH	577:628	The composition indicated that microencapsulation had a greater influence than the period of ripening since discrepancies were statistically significant (p<0.05) regarding the levels of protein, moisture, total solids and pH between the two cheeses both at the beginning and end of the ripening.
29433252	4	7	theme	ripening	498:505	arg1	period					488:493	the period	484:493	the period of ripening	484:505	The composition indicated that microencapsulation had a greater influence than the period of ripening since discrepancies were statistically significant (p<0.05) regarding the levels of protein, moisture, total solids and pH between the two cheeses both at the beginning and end of the ripening.
29433252	8	8	dep	they	1226:1229	arg1	ripen					1231:1235	ripen	1231:1235	ripen	1231:1235	The retarded time ranged between 2.32×10-2 and 2.38×10-2s and the Jo values were 29 times higher than the Ji values in the studied cheeses, indicating the loss of elasticity of the cheeses as they ripen.
29433252	1	9	theme	Lactococcus	277:287	arg1	lactis					289:294	Lactococcus lactis	277:294	Lactococcus lactis subsp	277:300	This study aimed at determining the composition and rheology changes during the ripening (60days) of Manchego-type cheeses prepared with Lactococcus lactis subsp.
29433252	3	10	theme	free	356:359	arg1	status					361:366	the free status	352:366	the free status	352:366	cremoris in the free status and microencapsulated in gellan gum.
29433252	8	11	theme	retarded	1038:1045	arg1	time					1047:1050	The retarded time	1034:1050	The retarded time	1034:1050	The retarded time ranged between 2.32×10-2 and 2.38×10-2s and the Jo values were 29 times higher than the Ji values in the studied cheeses, indicating the loss of elasticity of the cheeses as they ripen.
29433252	8	12	theme	studied	1157:1163	arg1	cheeses					1165:1171	the studied cheeses	1153:1171	the studied cheeses	1153:1171	The retarded time ranged between 2.32×10-2 and 2.38×10-2s and the Jo values were 29 times higher than the Ji values in the studied cheeses, indicating the loss of elasticity of the cheeses as they ripen.
29433252	1	13	theme	composition	176:186	arg1	changes					201:207	the composition and rheology changes	172:207	the composition and rheology changes during the ripening (60days) of Manchego-type cheeses prepared with Lactococcus lactis subsp	172:300	This study aimed at determining the composition and rheology changes during the ripening (60days) of Manchego-type cheeses prepared with Lactococcus lactis subsp.
29433252	4	14	theme	total	610:614	arg1	solids					616:621	total solids	610:621	total solids	610:621	The composition indicated that microencapsulation had a greater influence than the period of ripening since discrepancies were statistically significant (p<0.05) regarding the levels of protein, moisture, total solids and pH between the two cheeses both at the beginning and end of the ripening.
29433252	1	15	theme	rheology	192:199	arg1	changes					201:207	the composition and rheology changes	172:207	the composition and rheology changes during the ripening (60days) of Manchego-type cheeses prepared with Lactococcus lactis subsp	172:300	This study aimed at determining the composition and rheology changes during the ripening (60days) of Manchego-type cheeses prepared with Lactococcus lactis subsp.
29433252	1	16	theme	lactis	289:294	arg1	subsp					296:300	Lactococcus lactis subsp	277:300	Lactococcus lactis subsp	277:300	This study aimed at determining the composition and rheology changes during the ripening (60days) of Manchego-type cheeses prepared with Lactococcus lactis subsp.
29433252	8	17	theme	elasticity	1197:1206	arg1	loss					1189:1192	the loss	1185:1192	the loss of elasticity of the cheeses	1185:1221	The retarded time ranged between 2.32×10-2 and 2.38×10-2s and the Jo values were 29 times higher than the Ji values in the studied cheeses, indicating the loss of elasticity of the cheeses as they ripen.
29433252	7	18	theme	creep	923:927	arg1	curve					929:933	the creep curve	919:933	the creep curve	919:933	Conversely, the recovery percentages on the creep curve decreased in both cheeses with an increase in ripening as a result of the degradation of proteins.
29433252	7	19	from	percentages	904:914	arg1	curve					929:933	the creep curve	919:933	the creep curve	919:933	Conversely, the recovery percentages on the creep curve decreased in both cheeses with an increase in ripening as a result of the degradation of proteins.
29433252	5	20	theme	elastic	738:744	arg1	>G″					765:767	G'>G″	763:767	G'>G″	763:767	Both cheeses presented predominantly elastic characteristics (G'>G″).
29433252	5	20	theme	elastic	738:744	arg1	characteristics					746:760	predominantly elastic characteristics	724:760	predominantly elastic characteristics (G'>G″)	724:768	Both cheeses presented predominantly elastic characteristics (G'>G″).
29433252	4	21	contain	had	455:457	arg1	microencapsulation					436:453	microencapsulation	436:453	microencapsulation	436:453	The composition indicated that microencapsulation had a greater influence than the period of ripening since discrepancies were statistically significant (p<0.05) regarding the levels of protein, moisture, total solids and pH between the two cheeses both at the beginning and end of the ripening.
29433252	4	21	contain	had	455:457	arg2	influence					469:477	a greater influence	459:477	a greater influence	459:477	The composition indicated that microencapsulation had a greater influence than the period of ripening since discrepancies were statistically significant (p<0.05) regarding the levels of protein, moisture, total solids and pH between the two cheeses both at the beginning and end of the ripening.
29433252	2	22	theme	Lactococcus	314:324	arg1	lactis					326:331	Lactococcus lactis	314:331	Lactococcus lactis	314:331	lactis and Lactococcus lactis subsp.
29433252	3	23	theme	gellan	393:398	arg1	gum					400:402	gellan gum	393:402	gellan gum	393:402	cremoris in the free status and microencapsulated in gellan gum.
29433252	8	24	from	values	1143:1148	arg1	cheeses					1165:1171	the studied cheeses	1153:1171	the studied cheeses	1153:1171	The retarded time ranged between 2.32×10-2 and 2.38×10-2s and the Jo values were 29 times higher than the Ji values in the studied cheeses, indicating the loss of elasticity of the cheeses as they ripen.
29433252	6	25	theme	time	873:876	arg1	presence					833:840	the presence	829:840	the presence of microcapsules and, ripening time	829:876	The moduli and the viscosity significantly increased with the presence of microcapsules and, ripening time.
29433252	7	26	from	increase	969:976	arg1	ripening					981:988	ripening	981:988	ripening	981:988	Conversely, the recovery percentages on the creep curve decreased in both cheeses with an increase in ripening as a result of the degradation of proteins.
29433252	8	27	theme	cheeses	1215:1221	arg1	elasticity					1197:1206	elasticity	1197:1206	elasticity of the cheeses	1197:1221	The retarded time ranged between 2.32×10-2 and 2.38×10-2s and the Jo values were 29 times higher than the Ji values in the studied cheeses, indicating the loss of elasticity of the cheeses as they ripen.
29433252	0	28	theme	Lactococcus	37:47	arg1	lactis					49:54	free and microencapsulated Lactococcus lactis	10:54	free and microencapsulated Lactococcus lactis	10:54	Effect of free and microencapsulated Lactococcus lactis on composition and rheological properties of Manchego-type cheeses during ripening.
29433252	4	29	theme	protein	591:597	arg1	levels					581:586	the levels	577:586	the levels of protein, moisture, total solids and pH	577:628	The composition indicated that microencapsulation had a greater influence than the period of ripening since discrepancies were statistically significant (p<0.05) regarding the levels of protein, moisture, total solids and pH between the two cheeses both at the beginning and end of the ripening.
29433252	6	30	theme	ripening	864:871	arg1	time					873:876	ripening time	864:876	ripening time	864:876	The moduli and the viscosity significantly increased with the presence of microcapsules and, ripening time.
29433252	0	31	theme	microencapsulated	19:35	arg1	lactis					49:54	free and microencapsulated Lactococcus lactis	10:54	free and microencapsulated Lactococcus lactis	10:54	Effect of free and microencapsulated Lactococcus lactis on composition and rheological properties of Manchego-type cheeses during ripening.
29433252	8	32	theme	Ji	1140:1141	arg1	values					1143:1148	the Ji values	1136:1148	the Ji values in the studied cheeses	1136:1171	The retarded time ranged between 2.32×10-2 and 2.38×10-2s and the Jo values were 29 times higher than the Ji values in the studied cheeses, indicating the loss of elasticity of the cheeses as they ripen.
29433252	4	33	dep	significant	546:556	arg1	p<0.05					559:564	p<0.05	559:564	p<0.05	559:564	The composition indicated that microencapsulation had a greater influence than the period of ripening since discrepancies were statistically significant (p<0.05) regarding the levels of protein, moisture, total solids and pH between the two cheeses both at the beginning and end of the ripening.
29433252	7	34	theme	proteins	1024:1031	arg1	degradation					1009:1019	the degradation	1005:1019	the degradation of proteins	1005:1031	Conversely, the recovery percentages on the creep curve decreased in both cheeses with an increase in ripening as a result of the degradation of proteins.
29433252	4	35	dep	cheeses	646:652	arg1	both					654:657	both	654:657	both	654:657	The composition indicated that microencapsulation had a greater influence than the period of ripening since discrepancies were statistically significant (p<0.05) regarding the levels of protein, moisture, total solids and pH between the two cheeses both at the beginning and end of the ripening.
29433252	0	36	theme	lactis	49:54	arg1	Effect					0:5	Effect	0:5	Effect of free and microencapsulated Lactococcus lactis on composition and rheological properties of Manchego-type	0:113	Effect of free and microencapsulated Lactococcus lactis on composition and rheological properties of Manchego-type cheeses during ripening.
29433252	6	37	theme	microcapsules	845:857	arg1	presence					833:840	the presence	829:840	the presence of microcapsules and, ripening time	829:876	The moduli and the viscosity significantly increased with the presence of microcapsules and, ripening time.
29433252	7	38	theme	degradation	1009:1019	arg1	result					995:1000	a result	993:1000	a result of the degradation of proteins	993:1031	Conversely, the recovery percentages on the creep curve decreased in both cheeses with an increase in ripening as a result of the degradation of proteins.
29433252	3	39	from	cremoris	340:347	arg1	status					361:366	the free status	352:366	the free status	352:366	cremoris in the free status and microencapsulated in gellan gum.
29433252	2	40	dep	subsp	333:337	arg1	lactis					303:308	lactis	303:308	lactis	303:308	lactis and Lactococcus lactis subsp.
29433252	2	40	dep	subsp	333:337	arg1	lactis					326:331	Lactococcus lactis	314:331	Lactococcus lactis	314:331	lactis and Lactococcus lactis subsp.
29433252	4	41	theme	pH	627:628	arg1	levels					581:586	the levels	577:586	the levels of protein, moisture, total solids and pH	577:628	The composition indicated that microencapsulation had a greater influence than the period of ripening since discrepancies were statistically significant (p<0.05) regarding the levels of protein, moisture, total solids and pH between the two cheeses both at the beginning and end of the ripening.
29433252	8	42	theme	Jo	1100:1101	arg1	higher					1124:1129	higher	1124:1129	higher	1124:1129	The retarded time ranged between 2.32×10-2 and 2.38×10-2s and the Jo values were 29 times higher than the Ji values in the studied cheeses, indicating the loss of elasticity of the cheeses as they ripen.
29433252	8	42	theme	Jo	1100:1101	arg1	values					1103:1108	the Jo values	1096:1108	the Jo values	1096:1108	The retarded time ranged between 2.32×10-2 and 2.38×10-2s and the Jo values were 29 times higher than the Ji values in the studied cheeses, indicating the loss of elasticity of the cheeses as they ripen.
29433252	4	43	theme	greater	461:467	arg1	influence					469:477	a greater influence	459:477	a greater influence	459:477	The composition indicated that microencapsulation had a greater influence than the period of ripening since discrepancies were statistically significant (p<0.05) regarding the levels of protein, moisture, total solids and pH between the two cheeses both at the beginning and end of the ripening.
29433252	7	44	theme	recovery	895:902	arg1	percentages					904:914	the recovery percentages	891:914	the recovery percentages on the creep curve	891:933	Conversely, the recovery percentages on the creep curve decreased in both cheeses with an increase in ripening as a result of the degradation of proteins.
29433252	0	45	theme	free	10:13	arg1	lactis					49:54	free and microencapsulated Lactococcus lactis	10:54	free and microencapsulated Lactococcus lactis	10:54	Effect of free and microencapsulated Lactococcus lactis on composition and rheological properties of Manchego-type cheeses during ripening.
29433252	1	46	theme	Manchego-type	241:253	arg1	cheeses					255:261	Manchego-type cheeses	241:261	Manchego-type cheeses prepared with Lactococcus lactis subsp	241:300	This study aimed at determining the composition and rheology changes during the ripening (60days) of Manchego-type cheeses prepared with Lactococcus lactis subsp.
25261509	8	0	from	observations	1615:1626	arg1	structure					1660:1668	the community structure	1646:1668	the community structure	1646:1668	Combining the observations of differences in the community structure and the chemical and physical structure of the biomass, we hypothesize specific roles for individual community members in biomass deconstruction.
25261509	1	1	contain	have	164:167	arg1	communities					121:131	Microbial communities	111:131	Microbial communities that deconstruct plant biomass	111:162	Microbial communities that deconstruct plant biomass have broad relevance in biofuel production and global carbon cycling.
25261509	1	1	contain	have	164:167	arg2	relevance					175:183	broad relevance	169:183	broad relevance	169:183	Microbial communities that deconstruct plant biomass have broad relevance in biofuel production and global carbon cycling.
25261509	1	2	theme	biofuel	188:194	arg1	production					196:205	biofuel production	188:205	biofuel production	188:205	Microbial communities that deconstruct plant biomass have broad relevance in biofuel production and global carbon cycling.
25261509	4	3	dep	ionic-liquid	597:608	arg1	IL					611:612	IL	611:612	IL	611:612	Microbial communities were adapted to untreated, ammonium fiber expansion (AFEX)-pretreated, and ionic-liquid (IL)-pretreated SG under aerobic, thermophilic conditions using green waste compost as the inoculum to study biomass deconstruction by microbial consortia.
25261509	7	4	theme	rRNA	1270:1273	arg1	libraries					1289:1297	Small-subunit (SSU) rRNA gene amplicon libraries	1250:1297	Small-subunit (SSU) rRNA gene amplicon libraries	1250:1297	Small-subunit (SSU) rRNA gene amplicon libraries revealed that although the dominant taxa across these chemical pretreatments were consistently represented by members of the Firmicutes, the Bacteroidetes, and Deinococcus-Thermus, the abundance of selected operational taxonomic units (OTUs) varied, suggesting adaptations to the different substrates.
25261509	4	5	dep	aerobic	635:641	arg1	thermophilic					644:655	thermophilic	644:655	thermophilic	644:655	Microbial communities were adapted to untreated, ammonium fiber expansion (AFEX)-pretreated, and ionic-liquid (IL)-pretreated SG under aerobic, thermophilic conditions using green waste compost as the inoculum to study biomass deconstruction by microbial consortia.
25261509	8	6	theme	chemical	1678:1685	arg1	structure					1700:1708	the chemical and physical structure	1674:1708	the chemical and physical structure of the biomass	1674:1723	Combining the observations of differences in the community structure and the chemical and physical structure of the biomass, we hypothesize specific roles for individual community members in biomass deconstruction.
25261509	4	7	theme	aerobic	635:641	arg1	conditions					657:666	aerobic, thermophilic conditions	635:666	aerobic, thermophilic conditions using green waste compost as the inoculum to study biomass deconstruction by microbial consortia	635:763	Microbial communities were adapted to untreated, ammonium fiber expansion (AFEX)-pretreated, and ionic-liquid (IL)-pretreated SG under aerobic, thermophilic conditions using green waste compost as the inoculum to study biomass deconstruction by microbial consortia.
25261509	8	8	from	structure	1660:1668	arg1	structure					1700:1708	the chemical and physical structure	1674:1708	the chemical and physical structure of the biomass	1674:1723	Combining the observations of differences in the community structure and the chemical and physical structure of the biomass, we hypothesize specific roles for individual community members in biomass deconstruction.
25261509	8	8	from	structure	1660:1668	arg1	observations					1615:1626	the observations	1611:1626	the observations of differences in the community structure	1611:1668	Combining the observations of differences in the community structure and the chemical and physical structure of the biomass, we hypothesize specific roles for individual community members in biomass deconstruction.
25261509	4	9	theme	-pretreated	580:590	arg1	SG					626:627	untreated, ammonium fiber expansion (AFEX)-pretreated, and ionic-liquid (IL)-pretreated SG	538:627	untreated, ammonium fiber expansion (AFEX)-pretreated, and ionic-liquid (IL)-pretreated SG	538:627	Microbial communities were adapted to untreated, ammonium fiber expansion (AFEX)-pretreated, and ionic-liquid (IL)-pretreated SG under aerobic, thermophilic conditions using green waste compost as the inoculum to study biomass deconstruction by microbial consortia.
25261509	8	10	theme	biomass	1792:1798	arg1	deconstruction					1800:1813	biomass deconstruction	1792:1813	biomass deconstruction	1792:1813	Combining the observations of differences in the community structure and the chemical and physical structure of the biomass, we hypothesize specific roles for individual community members in biomass deconstruction.
25261509	7	11	theme	chemical	1353:1360	arg1	pretreatments					1362:1374	these chemical pretreatments	1347:1374	these chemical pretreatments	1347:1374	Small-subunit (SSU) rRNA gene amplicon libraries revealed that although the dominant taxa across these chemical pretreatments were consistently represented by members of the Firmicutes, the Bacteroidetes, and Deinococcus-Thermus, the abundance of selected operational taxonomic units (OTUs) varied, suggesting adaptations to the different substrates.
25261509	7	12	theme	SSU	1265:1267	arg1	rRNA					1270:1273	Small-subunit (SSU) rRNA	1250:1273	Small-subunit (SSU) rRNA gene amplicon libraries	1250:1297	Small-subunit (SSU) rRNA gene amplicon libraries revealed that although the dominant taxa across these chemical pretreatments were consistently represented by members of the Firmicutes, the Bacteroidetes, and Deinococcus-Thermus, the abundance of selected operational taxonomic units (OTUs) varied, suggesting adaptations to the different substrates.
25261509	5	13	theme	biomass	832:838	arg1	analysis					807:814	gravimetric analysis	795:814	gravimetric analysis of the residual biomass	795:838	After microbial cultivation, gravimetric analysis of the residual biomass demonstrated that both AFEX and IL pretreatment enhanced the deconstruction of the SG biomass approximately 2-fold.
25261509	6	14	theme	dominant	1113:1120	arg1	hydrolysis					1094:1103	polysaccharide hydrolysis	1079:1103	polysaccharide hydrolysis	1079:1103	Two-dimensional nuclear magnetic resonance (2D-NMR) experiments and acetyl bromide-reactive-lignin analysis indicated that polysaccharide hydrolysis was the dominant process occurring during microbial biomass deconstruction, and lignin remaining in the residual biomass was largely unmodified.
25261509	6	14	theme	dominant	1113:1120	arg1	process					1122:1128	the dominant process	1109:1128	the dominant process occurring during microbial biomass deconstruction	1109:1178	Two-dimensional nuclear magnetic resonance (2D-NMR) experiments and acetyl bromide-reactive-lignin analysis indicated that polysaccharide hydrolysis was the dominant process occurring during microbial biomass deconstruction, and lignin remaining in the residual biomass was largely unmodified.
25261509	8	15	theme	physical	1691:1698	arg1	structure					1700:1708	the chemical and physical structure	1674:1708	the chemical and physical structure of the biomass	1674:1723	Combining the observations of differences in the community structure and the chemical and physical structure of the biomass, we hypothesize specific roles for individual community members in biomass deconstruction.
25261509	7	16	theme	different	1579:1587	arg1	substrates					1589:1598	the different substrates	1575:1598	the different substrates	1575:1598	Small-subunit (SSU) rRNA gene amplicon libraries revealed that although the dominant taxa across these chemical pretreatments were consistently represented by members of the Firmicutes, the Bacteroidetes, and Deinococcus-Thermus, the abundance of selected operational taxonomic units (OTUs) varied, suggesting adaptations to the different substrates.
25261509	6	17	theme	residual	1209:1216	arg1	biomass					1218:1224	the residual biomass	1205:1224	the residual biomass	1205:1224	Two-dimensional nuclear magnetic resonance (2D-NMR) experiments and acetyl bromide-reactive-lignin analysis indicated that polysaccharide hydrolysis was the dominant process occurring during microbial biomass deconstruction, and lignin remaining in the residual biomass was largely unmodified.
25261509	4	18	theme	biomass	719:725	arg1	deconstruction					727:740	biomass deconstruction	719:740	biomass deconstruction	719:740	Microbial communities were adapted to untreated, ammonium fiber expansion (AFEX)-pretreated, and ionic-liquid (IL)-pretreated SG under aerobic, thermophilic conditions using green waste compost as the inoculum to study biomass deconstruction by microbial consortia.
25261509	5	19	theme	residual	823:830	arg1	biomass					832:838	the residual biomass	819:838	the residual biomass	819:838	After microbial cultivation, gravimetric analysis of the residual biomass demonstrated that both AFEX and IL pretreatment enhanced the deconstruction of the SG biomass approximately 2-fold.
25261509	6	20	theme	resonance	989:997	arg1	experiments					1008:1018	Two-dimensional nuclear magnetic resonance (2D-NMR) experiments	956:1018	Two-dimensional nuclear magnetic resonance (2D-NMR) experiments	956:1018	Two-dimensional nuclear magnetic resonance (2D-NMR) experiments and acetyl bromide-reactive-lignin analysis indicated that polysaccharide hydrolysis was the dominant process occurring during microbial biomass deconstruction, and lignin remaining in the residual biomass was largely unmodified.
25261509	0	21	theme	Consortia	57:65	arg1	Development					19:29	Substrate-Specific Development	0:29	Substrate-Specific Development of Thermophilic Bacterial Consortia	0:65	Substrate-Specific Development of Thermophilic Bacterial Consortia by Using Chemically Pretreated Switchgrass.
25261509	7	22	theme	gene	1275:1278	arg1	libraries					1289:1297	Small-subunit (SSU) rRNA gene amplicon libraries	1250:1297	Small-subunit (SSU) rRNA gene amplicon libraries	1250:1297	Small-subunit (SSU) rRNA gene amplicon libraries revealed that although the dominant taxa across these chemical pretreatments were consistently represented by members of the Firmicutes, the Bacteroidetes, and Deinococcus-Thermus, the abundance of selected operational taxonomic units (OTUs) varied, suggesting adaptations to the different substrates.
25261509	2	23	theme	hydrolysis	329:338	arg1	efficiency					305:314	increased efficiency	295:314	increased efficiency of enzymatic hydrolysis	295:338	Biomass pretreatments reduce plant biomass recalcitrance for increased efficiency of enzymatic hydrolysis.
25261509	1	24	theme	global	211:216	arg1	cycling					225:231	global carbon cycling	211:231	global carbon cycling	211:231	Microbial communities that deconstruct plant biomass have broad relevance in biofuel production and global carbon cycling.
25261509	3	25	theme	SG	463:464	arg1	biomass					467:473	switchgrass (SG) biomass	450:473	switchgrass (SG) biomass of various compositions	450:497	We exploited these chemical pretreatments to study how thermophilic bacterial consortia adapt to deconstruct switchgrass (SG) biomass of various compositions.
25261509	6	26	theme	magnetic	980:987	arg1	2D-NMR					1000:1005	2D-NMR	1000:1005	2D-NMR	1000:1005	Two-dimensional nuclear magnetic resonance (2D-NMR) experiments and acetyl bromide-reactive-lignin analysis indicated that polysaccharide hydrolysis was the dominant process occurring during microbial biomass deconstruction, and lignin remaining in the residual biomass was largely unmodified.
25261509	6	26	theme	magnetic	980:987	arg1	resonance					989:997	nuclear magnetic resonance	972:997	Two-dimensional nuclear magnetic resonance (2D-NMR) experiments	956:1018	Two-dimensional nuclear magnetic resonance (2D-NMR) experiments and acetyl bromide-reactive-lignin analysis indicated that polysaccharide hydrolysis was the dominant process occurring during microbial biomass deconstruction, and lignin remaining in the residual biomass was largely unmodified.
25261509	8	27	from	roles	1750:1754	arg1	deconstruction					1800:1813	biomass deconstruction	1792:1813	biomass deconstruction	1792:1813	Combining the observations of differences in the community structure and the chemical and physical structure of the biomass, we hypothesize specific roles for individual community members in biomass deconstruction.
25261509	3	28	theme	thermophilic	396:407	arg1	consortia					419:427	thermophilic bacterial consortia	396:427	thermophilic bacterial consortia	396:427	We exploited these chemical pretreatments to study how thermophilic bacterial consortia adapt to deconstruct switchgrass (SG) biomass of various compositions.
25261509	1	29	theme	Microbial	111:119	arg1	communities					121:131	Microbial communities	111:131	Microbial communities that deconstruct plant biomass	111:162	Microbial communities that deconstruct plant biomass have broad relevance in biofuel production and global carbon cycling.
25261509	1	30	theme	carbon	218:223	arg1	cycling					225:231	global carbon cycling	211:231	global carbon cycling	211:231	Microbial communities that deconstruct plant biomass have broad relevance in biofuel production and global carbon cycling.
25261509	0	31	theme	Substrate-Specific	0:17	arg1	Development					19:29	Substrate-Specific Development	0:29	Substrate-Specific Development of Thermophilic Bacterial Consortia	0:65	Substrate-Specific Development of Thermophilic Bacterial Consortia by Using Chemically Pretreated Switchgrass.
25261509	4	32	theme	-pretreated	614:624	arg1	SG					626:627	untreated, ammonium fiber expansion (AFEX)-pretreated, and ionic-liquid (IL)-pretreated SG	538:627	untreated, ammonium fiber expansion (AFEX)-pretreated, and ionic-liquid (IL)-pretreated SG	538:627	Microbial communities were adapted to untreated, ammonium fiber expansion (AFEX)-pretreated, and ionic-liquid (IL)-pretreated SG under aerobic, thermophilic conditions using green waste compost as the inoculum to study biomass deconstruction by microbial consortia.
25261509	8	33	from	structure	1700:1708	arg1	structure					1660:1668	the community structure	1646:1668	the community structure	1646:1668	Combining the observations of differences in the community structure and the chemical and physical structure of the biomass, we hypothesize specific roles for individual community members in biomass deconstruction.
25261509	3	34	theme	switchgrass	450:460	arg1	biomass					467:473	switchgrass (SG) biomass	450:473	switchgrass (SG) biomass of various compositions	450:497	We exploited these chemical pretreatments to study how thermophilic bacterial consortia adapt to deconstruct switchgrass (SG) biomass of various compositions.
25261509	8	35	theme	biomass	1717:1723	arg1	structure					1700:1708	the chemical and physical structure	1674:1708	the chemical and physical structure of the biomass	1674:1723	Combining the observations of differences in the community structure and the chemical and physical structure of the biomass, we hypothesize specific roles for individual community members in biomass deconstruction.
25261509	8	35	theme	biomass	1717:1723	arg1	observations					1615:1626	the observations	1611:1626	the observations of differences in the community structure	1611:1668	Combining the observations of differences in the community structure and the chemical and physical structure of the biomass, we hypothesize specific roles for individual community members in biomass deconstruction.
25261509	5	36	theme	microbial	772:780	arg1	cultivation					782:792	microbial cultivation	772:792	microbial cultivation	772:792	After microbial cultivation, gravimetric analysis of the residual biomass demonstrated that both AFEX and IL pretreatment enhanced the deconstruction of the SG biomass approximately 2-fold.
25261509	7	37	theme	taxonomic	1518:1526	arg1	units					1528:1532	selected operational taxonomic units (OTUs)	1497:1539	selected operational taxonomic units (OTUs)	1497:1539	Small-subunit (SSU) rRNA gene amplicon libraries revealed that although the dominant taxa across these chemical pretreatments were consistently represented by members of the Firmicutes, the Bacteroidetes, and Deinococcus-Thermus, the abundance of selected operational taxonomic units (OTUs) varied, suggesting adaptations to the different substrates.
25261509	7	37	theme	taxonomic	1518:1526	arg1	OTUs					1535:1538	OTUs	1535:1538	OTUs	1535:1538	Small-subunit (SSU) rRNA gene amplicon libraries revealed that although the dominant taxa across these chemical pretreatments were consistently represented by members of the Firmicutes, the Bacteroidetes, and Deinococcus-Thermus, the abundance of selected operational taxonomic units (OTUs) varied, suggesting adaptations to the different substrates.
25261509	6	38	theme	microbial	1147:1155	arg1	deconstruction					1165:1178	microbial biomass deconstruction	1147:1178	microbial biomass deconstruction	1147:1178	Two-dimensional nuclear magnetic resonance (2D-NMR) experiments and acetyl bromide-reactive-lignin analysis indicated that polysaccharide hydrolysis was the dominant process occurring during microbial biomass deconstruction, and lignin remaining in the residual biomass was largely unmodified.
25261509	7	39	theme	Small-subunit	1250:1262	arg1	rRNA					1270:1273	Small-subunit (SSU) rRNA	1250:1273	Small-subunit (SSU) rRNA gene amplicon libraries	1250:1297	Small-subunit (SSU) rRNA gene amplicon libraries revealed that although the dominant taxa across these chemical pretreatments were consistently represented by members of the Firmicutes, the Bacteroidetes, and Deinococcus-Thermus, the abundance of selected operational taxonomic units (OTUs) varied, suggesting adaptations to the different substrates.
25261509	7	40	theme	Deinococcus-Thermus	1459:1477	arg1	members					1409:1415	members	1409:1415	members of the Firmicutes, the Bacteroidetes, and Deinococcus-Thermus	1409:1477	Small-subunit (SSU) rRNA gene amplicon libraries revealed that although the dominant taxa across these chemical pretreatments were consistently represented by members of the Firmicutes, the Bacteroidetes, and Deinococcus-Thermus, the abundance of selected operational taxonomic units (OTUs) varied, suggesting adaptations to the different substrates.
25261509	6	41	theme	nuclear	972:978	arg1	2D-NMR					1000:1005	2D-NMR	1000:1005	2D-NMR	1000:1005	Two-dimensional nuclear magnetic resonance (2D-NMR) experiments and acetyl bromide-reactive-lignin analysis indicated that polysaccharide hydrolysis was the dominant process occurring during microbial biomass deconstruction, and lignin remaining in the residual biomass was largely unmodified.
25261509	6	41	theme	nuclear	972:978	arg1	resonance					989:997	nuclear magnetic resonance	972:997	Two-dimensional nuclear magnetic resonance (2D-NMR) experiments	956:1018	Two-dimensional nuclear magnetic resonance (2D-NMR) experiments and acetyl bromide-reactive-lignin analysis indicated that polysaccharide hydrolysis was the dominant process occurring during microbial biomass deconstruction, and lignin remaining in the residual biomass was largely unmodified.
25261509	5	42	theme	SG	923:924	arg1	biomass					926:932	the SG biomass	919:932	the SG biomass	919:932	After microbial cultivation, gravimetric analysis of the residual biomass demonstrated that both AFEX and IL pretreatment enhanced the deconstruction of the SG biomass approximately 2-fold.
25261509	3	43	theme	various	478:484	arg1	compositions					486:497	various compositions	478:497	various compositions	478:497	We exploited these chemical pretreatments to study how thermophilic bacterial consortia adapt to deconstruct switchgrass (SG) biomass of various compositions.
25261509	6	44	theme	polysaccharide	1079:1092	arg1	hydrolysis					1094:1103	polysaccharide hydrolysis	1079:1103	polysaccharide hydrolysis	1079:1103	Two-dimensional nuclear magnetic resonance (2D-NMR) experiments and acetyl bromide-reactive-lignin analysis indicated that polysaccharide hydrolysis was the dominant process occurring during microbial biomass deconstruction, and lignin remaining in the residual biomass was largely unmodified.
25261509	6	44	theme	polysaccharide	1079:1092	arg1	process					1122:1128	the dominant process	1109:1128	the dominant process occurring during microbial biomass deconstruction	1109:1178	Two-dimensional nuclear magnetic resonance (2D-NMR) experiments and acetyl bromide-reactive-lignin analysis indicated that polysaccharide hydrolysis was the dominant process occurring during microbial biomass deconstruction, and lignin remaining in the residual biomass was largely unmodified.
25261509	6	45	theme	Two-dimensional	956:970	arg1	experiments					1008:1018	Two-dimensional nuclear magnetic resonance (2D-NMR) experiments	956:1018	Two-dimensional nuclear magnetic resonance (2D-NMR) experiments	956:1018	Two-dimensional nuclear magnetic resonance (2D-NMR) experiments and acetyl bromide-reactive-lignin analysis indicated that polysaccharide hydrolysis was the dominant process occurring during microbial biomass deconstruction, and lignin remaining in the residual biomass was largely unmodified.
25261509	5	46	theme	biomass	926:932	arg1	deconstruction					901:914	the deconstruction	897:914	the deconstruction of the SG biomass	897:932	After microbial cultivation, gravimetric analysis of the residual biomass demonstrated that both AFEX and IL pretreatment enhanced the deconstruction of the SG biomass approximately 2-fold.
25261509	0	47	theme	Bacterial	47:55	arg1	Consortia					57:65	Thermophilic Bacterial Consortia	34:65	Thermophilic Bacterial Consortia	34:65	Substrate-Specific Development of Thermophilic Bacterial Consortia by Using Chemically Pretreated Switchgrass.
25261509	4	48	theme	ionic-liquid	597:608	arg1	SG					626:627	untreated, ammonium fiber expansion (AFEX)-pretreated, and ionic-liquid (IL)-pretreated SG	538:627	untreated, ammonium fiber expansion (AFEX)-pretreated, and ionic-liquid (IL)-pretreated SG	538:627	Microbial communities were adapted to untreated, ammonium fiber expansion (AFEX)-pretreated, and ionic-liquid (IL)-pretreated SG under aerobic, thermophilic conditions using green waste compost as the inoculum to study biomass deconstruction by microbial consortia.
25261509	3	49	theme	compositions	486:497	arg1	biomass					467:473	switchgrass (SG) biomass	450:473	switchgrass (SG) biomass of various compositions	450:497	We exploited these chemical pretreatments to study how thermophilic bacterial consortia adapt to deconstruct switchgrass (SG) biomass of various compositions.
25261509	8	50	theme	differences	1631:1641	arg1	structure					1700:1708	the chemical and physical structure	1674:1708	the chemical and physical structure of the biomass	1674:1723	Combining the observations of differences in the community structure and the chemical and physical structure of the biomass, we hypothesize specific roles for individual community members in biomass deconstruction.
25261509	8	50	theme	differences	1631:1641	arg1	observations					1615:1626	the observations	1611:1626	the observations of differences in the community structure	1611:1668	Combining the observations of differences in the community structure and the chemical and physical structure of the biomass, we hypothesize specific roles for individual community members in biomass deconstruction.
25261509	0	51	theme	Thermophilic	34:45	arg1	Consortia					57:65	Thermophilic Bacterial Consortia	34:65	Thermophilic Bacterial Consortia	34:65	Substrate-Specific Development of Thermophilic Bacterial Consortia by Using Chemically Pretreated Switchgrass.
25261509	5	52	theme	IL	872:873	arg1	pretreatment					875:886	IL pretreatment	872:886	IL pretreatment	872:886	After microbial cultivation, gravimetric analysis of the residual biomass demonstrated that both AFEX and IL pretreatment enhanced the deconstruction of the SG biomass approximately 2-fold.
25261509	8	53	theme	specific	1741:1748	arg1	roles					1750:1754	specific roles	1741:1754	specific roles for individual community members in biomass deconstruction	1741:1813	Combining the observations of differences in the community structure and the chemical and physical structure of the biomass, we hypothesize specific roles for individual community members in biomass deconstruction.
25261509	4	54	theme	untreated	538:546	arg1	SG					626:627	untreated, ammonium fiber expansion (AFEX)-pretreated, and ionic-liquid (IL)-pretreated SG	538:627	untreated, ammonium fiber expansion (AFEX)-pretreated, and ionic-liquid (IL)-pretreated SG	538:627	Microbial communities were adapted to untreated, ammonium fiber expansion (AFEX)-pretreated, and ionic-liquid (IL)-pretreated SG under aerobic, thermophilic conditions using green waste compost as the inoculum to study biomass deconstruction by microbial consortia.
25261509	2	55	theme	biomass	269:275	arg1	recalcitrance					277:289	plant biomass recalcitrance	263:289	plant biomass recalcitrance	263:289	Biomass pretreatments reduce plant biomass recalcitrance for increased efficiency of enzymatic hydrolysis.
25261509	1	56	theme	plant	150:154	arg1	biomass					156:162	plant biomass	150:162	plant biomass	150:162	Microbial communities that deconstruct plant biomass have broad relevance in biofuel production and global carbon cycling.
25261509	5	57	theme	gravimetric	795:805	arg1	analysis					807:814	gravimetric analysis	795:814	gravimetric analysis of the residual biomass	795:838	After microbial cultivation, gravimetric analysis of the residual biomass demonstrated that both AFEX and IL pretreatment enhanced the deconstruction of the SG biomass approximately 2-fold.
25261509	8	58	theme	individual	1760:1769	arg1	members					1781:1787	individual community members	1760:1787	individual community members	1760:1787	Combining the observations of differences in the community structure and the chemical and physical structure of the biomass, we hypothesize specific roles for individual community members in biomass deconstruction.
25261509	4	59	theme	Microbial	500:508	arg1	communities					510:520	Microbial communities	500:520	Microbial communities	500:520	Microbial communities were adapted to untreated, ammonium fiber expansion (AFEX)-pretreated, and ionic-liquid (IL)-pretreated SG under aerobic, thermophilic conditions using green waste compost as the inoculum to study biomass deconstruction by microbial consortia.
25261509	2	60	theme	plant	263:267	arg1	recalcitrance					277:289	plant biomass recalcitrance	263:289	plant biomass recalcitrance	263:289	Biomass pretreatments reduce plant biomass recalcitrance for increased efficiency of enzymatic hydrolysis.
25261509	7	61	theme	Bacteroidetes	1440:1452	arg1	members					1409:1415	members	1409:1415	members of the Firmicutes, the Bacteroidetes, and Deinococcus-Thermus	1409:1477	Small-subunit (SSU) rRNA gene amplicon libraries revealed that although the dominant taxa across these chemical pretreatments were consistently represented by members of the Firmicutes, the Bacteroidetes, and Deinococcus-Thermus, the abundance of selected operational taxonomic units (OTUs) varied, suggesting adaptations to the different substrates.
25261509	6	62	theme	bromide-reactive-lignin	1031:1053	arg1	analysis					1055:1062	acetyl bromide-reactive-lignin analysis	1024:1062	acetyl bromide-reactive-lignin analysis	1024:1062	Two-dimensional nuclear magnetic resonance (2D-NMR) experiments and acetyl bromide-reactive-lignin analysis indicated that polysaccharide hydrolysis was the dominant process occurring during microbial biomass deconstruction, and lignin remaining in the residual biomass was largely unmodified.
25261509	7	63	theme	amplicon	1280:1287	arg1	libraries					1289:1297	Small-subunit (SSU) rRNA gene amplicon libraries	1250:1297	Small-subunit (SSU) rRNA gene amplicon libraries	1250:1297	Small-subunit (SSU) rRNA gene amplicon libraries revealed that although the dominant taxa across these chemical pretreatments were consistently represented by members of the Firmicutes, the Bacteroidetes, and Deinococcus-Thermus, the abundance of selected operational taxonomic units (OTUs) varied, suggesting adaptations to the different substrates.
25261509	7	64	theme	units	1528:1532	arg1	abundance					1484:1492	the abundance	1480:1492	the abundance of selected operational taxonomic units (OTUs)	1480:1539	Small-subunit (SSU) rRNA gene amplicon libraries revealed that although the dominant taxa across these chemical pretreatments were consistently represented by members of the Firmicutes, the Bacteroidetes, and Deinococcus-Thermus, the abundance of selected operational taxonomic units (OTUs) varied, suggesting adaptations to the different substrates.
25261509	2	65	theme	enzymatic	319:327	arg1	hydrolysis					329:338	enzymatic hydrolysis	319:338	enzymatic hydrolysis	319:338	Biomass pretreatments reduce plant biomass recalcitrance for increased efficiency of enzymatic hydrolysis.
25261509	7	66	theme	Firmicutes	1424:1433	arg1	members					1409:1415	members	1409:1415	members of the Firmicutes, the Bacteroidetes, and Deinococcus-Thermus	1409:1477	Small-subunit (SSU) rRNA gene amplicon libraries revealed that although the dominant taxa across these chemical pretreatments were consistently represented by members of the Firmicutes, the Bacteroidetes, and Deinococcus-Thermus, the abundance of selected operational taxonomic units (OTUs) varied, suggesting adaptations to the different substrates.
25261509	4	67	theme	green	674:678	arg1	inoculum					701:708	the inoculum to study biomass deconstruction by microbial consortia	697:763	the inoculum to study biomass deconstruction by microbial consortia	697:763	Microbial communities were adapted to untreated, ammonium fiber expansion (AFEX)-pretreated, and ionic-liquid (IL)-pretreated SG under aerobic, thermophilic conditions using green waste compost as the inoculum to study biomass deconstruction by microbial consortia.
25261509	4	67	theme	green	674:678	arg1	compost					686:692	green waste compost	674:692	green waste compost	674:692	Microbial communities were adapted to untreated, ammonium fiber expansion (AFEX)-pretreated, and ionic-liquid (IL)-pretreated SG under aerobic, thermophilic conditions using green waste compost as the inoculum to study biomass deconstruction by microbial consortia.
25261509	3	68	theme	chemical	360:367	arg1	pretreatments					369:381	these chemical pretreatments	354:381	these chemical pretreatments	354:381	We exploited these chemical pretreatments to study how thermophilic bacterial consortia adapt to deconstruct switchgrass (SG) biomass of various compositions.
25261509	2	69	theme	Biomass	234:240	arg1	pretreatments					242:254	Biomass pretreatments	234:254	Biomass pretreatments	234:254	Biomass pretreatments reduce plant biomass recalcitrance for increased efficiency of enzymatic hydrolysis.
25261509	8	70	from	differences	1631:1641	arg1	structure					1660:1668	the community structure	1646:1668	the community structure	1646:1668	Combining the observations of differences in the community structure and the chemical and physical structure of the biomass, we hypothesize specific roles for individual community members in biomass deconstruction.
25261509	1	71	theme	broad	169:173	arg1	relevance					175:183	broad relevance	169:183	broad relevance	169:183	Microbial communities that deconstruct plant biomass have broad relevance in biofuel production and global carbon cycling.
25261509	8	72	theme	community	1771:1779	arg1	members					1781:1787	individual community members	1760:1787	individual community members	1760:1787	Combining the observations of differences in the community structure and the chemical and physical structure of the biomass, we hypothesize specific roles for individual community members in biomass deconstruction.
25261509	0	73	theme	Pretreated	87:96	arg1	Switchgrass					98:108	Chemically Pretreated Switchgrass	76:108	Chemically Pretreated Switchgrass	76:108	Substrate-Specific Development of Thermophilic Bacterial Consortia by Using Chemically Pretreated Switchgrass.
25261509	8	74	theme	community	1650:1658	arg1	structure					1660:1668	the community structure	1646:1668	the community structure	1646:1668	Combining the observations of differences in the community structure and the chemical and physical structure of the biomass, we hypothesize specific roles for individual community members in biomass deconstruction.
25261509	7	75	theme	operational	1506:1516	arg1	units					1528:1532	selected operational taxonomic units (OTUs)	1497:1539	selected operational taxonomic units (OTUs)	1497:1539	Small-subunit (SSU) rRNA gene amplicon libraries revealed that although the dominant taxa across these chemical pretreatments were consistently represented by members of the Firmicutes, the Bacteroidetes, and Deinococcus-Thermus, the abundance of selected operational taxonomic units (OTUs) varied, suggesting adaptations to the different substrates.
25261509	7	75	theme	operational	1506:1516	arg1	OTUs					1535:1538	OTUs	1535:1538	OTUs	1535:1538	Small-subunit (SSU) rRNA gene amplicon libraries revealed that although the dominant taxa across these chemical pretreatments were consistently represented by members of the Firmicutes, the Bacteroidetes, and Deinococcus-Thermus, the abundance of selected operational taxonomic units (OTUs) varied, suggesting adaptations to the different substrates.
25261509	7	76	theme	selected	1497:1504	arg1	units					1528:1532	selected operational taxonomic units (OTUs)	1497:1539	selected operational taxonomic units (OTUs)	1497:1539	Small-subunit (SSU) rRNA gene amplicon libraries revealed that although the dominant taxa across these chemical pretreatments were consistently represented by members of the Firmicutes, the Bacteroidetes, and Deinococcus-Thermus, the abundance of selected operational taxonomic units (OTUs) varied, suggesting adaptations to the different substrates.
25261509	7	76	theme	selected	1497:1504	arg1	OTUs					1535:1538	OTUs	1535:1538	OTUs	1535:1538	Small-subunit (SSU) rRNA gene amplicon libraries revealed that although the dominant taxa across these chemical pretreatments were consistently represented by members of the Firmicutes, the Bacteroidetes, and Deinococcus-Thermus, the abundance of selected operational taxonomic units (OTUs) varied, suggesting adaptations to the different substrates.
25261509	6	77	theme	biomass	1157:1163	arg1	deconstruction					1165:1178	microbial biomass deconstruction	1147:1178	microbial biomass deconstruction	1147:1178	Two-dimensional nuclear magnetic resonance (2D-NMR) experiments and acetyl bromide-reactive-lignin analysis indicated that polysaccharide hydrolysis was the dominant process occurring during microbial biomass deconstruction, and lignin remaining in the residual biomass was largely unmodified.
25261509	3	78	theme	bacterial	409:417	arg1	consortia					419:427	thermophilic bacterial consortia	396:427	thermophilic bacterial consortia	396:427	We exploited these chemical pretreatments to study how thermophilic bacterial consortia adapt to deconstruct switchgrass (SG) biomass of various compositions.
25261509	6	79	theme	acetyl	1024:1029	arg1	analysis					1055:1062	acetyl bromide-reactive-lignin analysis	1024:1062	acetyl bromide-reactive-lignin analysis	1024:1062	Two-dimensional nuclear magnetic resonance (2D-NMR) experiments and acetyl bromide-reactive-lignin analysis indicated that polysaccharide hydrolysis was the dominant process occurring during microbial biomass deconstruction, and lignin remaining in the residual biomass was largely unmodified.
25261509	2	80	theme	increased	295:303	arg1	efficiency					305:314	increased efficiency	295:314	increased efficiency of enzymatic hydrolysis	295:338	Biomass pretreatments reduce plant biomass recalcitrance for increased efficiency of enzymatic hydrolysis.
25261509	4	81	theme	microbial	745:753	arg1	consortia					755:763	microbial consortia	745:763	microbial consortia	745:763	Microbial communities were adapted to untreated, ammonium fiber expansion (AFEX)-pretreated, and ionic-liquid (IL)-pretreated SG under aerobic, thermophilic conditions using green waste compost as the inoculum to study biomass deconstruction by microbial consortia.
25261509	7	82	theme	dominant	1326:1333	arg1	taxa					1335:1338	the dominant taxa	1322:1338	the dominant taxa across these chemical pretreatments	1322:1374	Small-subunit (SSU) rRNA gene amplicon libraries revealed that although the dominant taxa across these chemical pretreatments were consistently represented by members of the Firmicutes, the Bacteroidetes, and Deinococcus-Thermus, the abundance of selected operational taxonomic units (OTUs) varied, suggesting adaptations to the different substrates.
25261509	4	83	theme	waste	680:684	arg1	inoculum					701:708	the inoculum to study biomass deconstruction by microbial consortia	697:763	the inoculum to study biomass deconstruction by microbial consortia	697:763	Microbial communities were adapted to untreated, ammonium fiber expansion (AFEX)-pretreated, and ionic-liquid (IL)-pretreated SG under aerobic, thermophilic conditions using green waste compost as the inoculum to study biomass deconstruction by microbial consortia.
25261509	4	83	theme	waste	680:684	arg1	compost					686:692	green waste compost	674:692	green waste compost	674:692	Microbial communities were adapted to untreated, ammonium fiber expansion (AFEX)-pretreated, and ionic-liquid (IL)-pretreated SG under aerobic, thermophilic conditions using green waste compost as the inoculum to study biomass deconstruction by microbial consortia.
26413701	3	0	dep	the	391:393	arg1	both					395:398	both	395:398	both	395:398	The electrical resistivity of the fiber is changed according to the strain variation applied to the both ends of the specimen.
26413701	4	1	theme	suitable	549:556	arg1	material					558:565	a suitable material	547:565	a suitable material for delicate force control in sensing work	547:608	The sensor built by using the CPF has a higher gauge factor (4) compared to conventional metal strain gauges (~2) indicating a suitable material for delicate force control in sensing work.
26413701	1	2	theme	composited	69:78	arg1	fiber					80:84	A chitosan/polypyrrole composited fiber	46:84	A chitosan/polypyrrole composited fiber as bio-compatible materials for artificial muscles	46:135	A chitosan/polypyrrole composited fiber as bio-compatible materials for artificial muscles is investigated.
26413701	4	3	theme	gauge	469:473	arg1	factor					475:480	a higher gauge factor	460:480	a higher gauge factor (4)	460:484	The sensor built by using the CPF has a higher gauge factor (4) compared to conventional metal strain gauges (~2) indicating a suitable material for delicate force control in sensing work.
26413701	3	4	theme	strain	363:368	arg1	variation					370:378	the strain variation	359:378	the strain variation applied to the both ends of the specimen	359:419	The electrical resistivity of the fiber is changed according to the strain variation applied to the both ends of the specimen.
26413701	4	5	theme	higher	462:467	arg1	factor					475:480	a higher gauge factor	460:480	a higher gauge factor (4)	460:484	The sensor built by using the CPF has a higher gauge factor (4) compared to conventional metal strain gauges (~2) indicating a suitable material for delicate force control in sensing work.
26413701	1	6	theme	bio-compatible	89:102	arg1	materials					104:112	bio-compatible materials	89:112	bio-compatible materials for artificial muscles	89:135	A chitosan/polypyrrole composited fiber as bio-compatible materials for artificial muscles is investigated.
26413701	2	7	theme	chitosan/polypyrrole	158:177	arg1	fiber					179:183	The chitosan/polypyrrole fiber	154:183	The chitosan/polypyrrole fiber (CPF)	154:189	The chitosan/polypyrrole fiber (CPF) is fabricated by in-situ chemical polymerization of pyrrole monomer solution using FeCl3 as an oxidant.
26413701	2	7	theme	chitosan/polypyrrole	158:177	arg1	CPF					186:188	CPF	186:188	CPF	186:188	The chitosan/polypyrrole fiber (CPF) is fabricated by in-situ chemical polymerization of pyrrole monomer solution using FeCl3 as an oxidant.
26413701	0	8	theme	Strain	31:36	arg1	Sensor					38:43	Strain Sensor	31:43	Strain Sensor	31:43	Chitosan-Polypyrrole Fiber for Strain Sensor.
26413701	3	9	theme	electrical	299:308	arg1	resistivity					310:320	The electrical resistivity	295:320	The electrical resistivity of the fiber	295:333	The electrical resistivity of the fiber is changed according to the strain variation applied to the both ends of the specimen.
26413701	1	10	theme	artificial	118:127	arg1	muscles					129:135	artificial muscles	118:135	artificial muscles	118:135	A chitosan/polypyrrole composited fiber as bio-compatible materials for artificial muscles is investigated.
26413701	4	11	contain	has	456:458	arg1	sensor					426:431	The sensor	422:431	The sensor built by using the CPF	422:454	The sensor built by using the CPF has a higher gauge factor (4) compared to conventional metal strain gauges (~2) indicating a suitable material for delicate force control in sensing work.
26413701	4	11	contain	has	456:458	arg2	factor					475:480	a higher gauge factor	460:480	a higher gauge factor (4)	460:484	The sensor built by using the CPF has a higher gauge factor (4) compared to conventional metal strain gauges (~2) indicating a suitable material for delicate force control in sensing work.
26413701	4	12	theme	sensing	597:603	arg1	work					605:608	sensing work	597:608	sensing work	597:608	The sensor built by using the CPF has a higher gauge factor (4) compared to conventional metal strain gauges (~2) indicating a suitable material for delicate force control in sensing work.
26413701	4	13	theme	metal	511:515	arg1	~2					532:533	~2	532:533	~2	532:533	The sensor built by using the CPF has a higher gauge factor (4) compared to conventional metal strain gauges (~2) indicating a suitable material for delicate force control in sensing work.
26413701	4	13	theme	metal	511:515	arg1	gauges					524:529	conventional metal strain gauges	498:529	conventional metal strain gauges (~2) indicating a suitable material for delicate force control in sensing work	498:608	The sensor built by using the CPF has a higher gauge factor (4) compared to conventional metal strain gauges (~2) indicating a suitable material for delicate force control in sensing work.
26413701	2	14	theme	solution	259:266	arg1	polymerization					225:238	in-situ chemical polymerization	208:238	in-situ chemical polymerization of pyrrole monomer solution using FeCl3 as an oxidant	208:292	The chitosan/polypyrrole fiber (CPF) is fabricated by in-situ chemical polymerization of pyrrole monomer solution using FeCl3 as an oxidant.
26413701	4	15	theme	conventional	498:509	arg1	~2					532:533	~2	532:533	~2	532:533	The sensor built by using the CPF has a higher gauge factor (4) compared to conventional metal strain gauges (~2) indicating a suitable material for delicate force control in sensing work.
26413701	4	15	theme	conventional	498:509	arg1	gauges					524:529	conventional metal strain gauges	498:529	conventional metal strain gauges (~2) indicating a suitable material for delicate force control in sensing work	498:608	The sensor built by using the CPF has a higher gauge factor (4) compared to conventional metal strain gauges (~2) indicating a suitable material for delicate force control in sensing work.
26413701	3	16	theme	fiber	329:333	arg1	resistivity					310:320	The electrical resistivity	295:320	The electrical resistivity of the fiber	295:333	The electrical resistivity of the fiber is changed according to the strain variation applied to the both ends of the specimen.
26413701	2	17	theme	monomer	251:257	arg1	solution					259:266	pyrrole monomer solution	243:266	pyrrole monomer solution using FeCl3 as an oxidant	243:292	The chitosan/polypyrrole fiber (CPF) is fabricated by in-situ chemical polymerization of pyrrole monomer solution using FeCl3 as an oxidant.
26413701	2	18	theme	pyrrole	243:249	arg1	solution					259:266	pyrrole monomer solution	243:266	pyrrole monomer solution using FeCl3 as an oxidant	243:292	The chitosan/polypyrrole fiber (CPF) is fabricated by in-situ chemical polymerization of pyrrole monomer solution using FeCl3 as an oxidant.
26413701	4	19	theme	strain	517:522	arg1	~2					532:533	~2	532:533	~2	532:533	The sensor built by using the CPF has a higher gauge factor (4) compared to conventional metal strain gauges (~2) indicating a suitable material for delicate force control in sensing work.
26413701	4	19	theme	strain	517:522	arg1	gauges					524:529	conventional metal strain gauges	498:529	conventional metal strain gauges (~2) indicating a suitable material for delicate force control in sensing work	498:608	The sensor built by using the CPF has a higher gauge factor (4) compared to conventional metal strain gauges (~2) indicating a suitable material for delicate force control in sensing work.
26413701	4	20	theme	force	580:584	arg1	control					586:592	delicate force control	571:592	delicate force control in sensing work	571:608	The sensor built by using the CPF has a higher gauge factor (4) compared to conventional metal strain gauges (~2) indicating a suitable material for delicate force control in sensing work.
26413701	2	21	theme	chemical	216:223	arg1	polymerization					225:238	in-situ chemical polymerization	208:238	in-situ chemical polymerization of pyrrole monomer solution using FeCl3 as an oxidant	208:292	The chitosan/polypyrrole fiber (CPF) is fabricated by in-situ chemical polymerization of pyrrole monomer solution using FeCl3 as an oxidant.
26413701	4	22	theme	delicate	571:578	arg1	control					586:592	delicate force control	571:592	delicate force control in sensing work	571:608	The sensor built by using the CPF has a higher gauge factor (4) compared to conventional metal strain gauges (~2) indicating a suitable material for delicate force control in sensing work.
26413701	2	23	theme	in-situ	208:214	arg1	polymerization					225:238	in-situ chemical polymerization	208:238	in-situ chemical polymerization of pyrrole monomer solution using FeCl3 as an oxidant	208:292	The chitosan/polypyrrole fiber (CPF) is fabricated by in-situ chemical polymerization of pyrrole monomer solution using FeCl3 as an oxidant.
26413701	4	24	from	control	586:592	arg1	work					605:608	sensing work	597:608	sensing work	597:608	The sensor built by using the CPF has a higher gauge factor (4) compared to conventional metal strain gauges (~2) indicating a suitable material for delicate force control in sensing work.
26413701	1	25	theme	chitosan/polypyrrole	48:67	arg1	fiber					80:84	A chitosan/polypyrrole composited fiber	46:84	A chitosan/polypyrrole composited fiber as bio-compatible materials for artificial muscles	46:135	A chitosan/polypyrrole composited fiber as bio-compatible materials for artificial muscles is investigated.
25647718	7	0	theme	seaweeds	1645:1652	arg1	characteristics					1618:1632	the chemical characteristics	1605:1632	the chemical characteristics of diverse seaweeds based on large chemical data sets, particularly complicated overlapping spectral data	1605:1738	These results suggest that this integrated analytical strategy is useful for extracting and identifying the chemical characteristics of diverse seaweeds based on large chemical data sets, particularly complicated overlapping spectral data.
25647718	4	1	theme	multivariate	935:946	arg1	methods					1001:1007	multivariate curve resolution-alternating least-squares (MCR-ALS) methods	935:1007	multivariate curve resolution-alternating least-squares (MCR-ALS) methods	935:1007	The spectral data were preprocessed using non-negative matrix factorization (NMF) and NMF combined with multivariate curve resolution-alternating least-squares (MCR-ALS) methods in order to separate individual component information from the overlapping and/or broad spectral peaks.
25647718	6	2	theme	metal	1309:1313	arg1	cellulose					1378:1386	cellulose	1378:1386	cellulose	1378:1386	Further network analysis revealed a close correlation between the heavy metal elements and characteristic components of brown algae, such as cellulose, alginic acid, and sulfated mucopolysaccharides, providing a componential basis for its metal-sorbing potential.
25647718	6	2	theme	metal	1309:1313	arg1	acid					1397:1400	alginic acid	1389:1400	alginic acid	1389:1400	Further network analysis revealed a close correlation between the heavy metal elements and characteristic components of brown algae, such as cellulose, alginic acid, and sulfated mucopolysaccharides, providing a componential basis for its metal-sorbing potential.
25647718	6	2	theme	metal	1309:1313	arg1	elements					1315:1322	heavy metal elements	1303:1322	heavy metal elements	1303:1322	Further network analysis revealed a close correlation between the heavy metal elements and characteristic components of brown algae, such as cellulose, alginic acid, and sulfated mucopolysaccharides, providing a componential basis for its metal-sorbing potential.
25647718	6	2	theme	metal	1309:1313	arg1	mucopolysaccharides					1416:1434	sulfated mucopolysaccharides	1407:1434	sulfated mucopolysaccharides	1407:1434	Further network analysis revealed a close correlation between the heavy metal elements and characteristic components of brown algae, such as cellulose, alginic acid, and sulfated mucopolysaccharides, providing a componential basis for its metal-sorbing potential.
25647718	6	3	theme	alginic	1389:1395	arg1	acid					1397:1400	alginic acid	1389:1400	alginic acid	1389:1400	Further network analysis revealed a close correlation between the heavy metal elements and characteristic components of brown algae, such as cellulose, alginic acid, and sulfated mucopolysaccharides, providing a componential basis for its metal-sorbing potential.
25647718	3	4	theme	resonance	598:606	arg1	spectrometry					809:820	isotope ratio mass spectrometry	790:820	isotope ratio mass spectrometry (IR-MS)	790:828	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	3	4	theme	resonance	598:606	arg1	analysis					666:673	thermogravimetry-differential thermal analysis	628:673	thermogravimetry-differential thermal analysis (TG-DTA)	628:682	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	3	4	theme	resonance	598:606	arg1	spectrometry					729:740	plasma-optical emission spectrometry	705:740	plasma-optical emission spectrometry (ICP-OES)	705:750	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	3	4	theme	resonance	598:606	arg1	analysis					776:783	CHNS/O total elemental analysis	753:783	CHNS/O total elemental analysis	753:783	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	3	4	theme	resonance	598:606	arg1	spectroscopy					614:625	infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy	534:625	infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy	534:625	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	7	5	theme	chemical	1609:1616	arg1	characteristics					1618:1632	the chemical characteristics	1605:1632	the chemical characteristics of diverse seaweeds based on large chemical data sets, particularly complicated overlapping spectral data	1605:1738	These results suggest that this integrated analytical strategy is useful for extracting and identifying the chemical characteristics of diverse seaweeds based on large chemical data sets, particularly complicated overlapping spectral data.
25647718	2	6	theme	novel	277:281	arg1	strategies					305:314	novel integrated analytical strategies	277:314	novel integrated analytical strategies for comprehensively characterizing seaweed similarities based on chemical diversity	277:398	The present study was aimed at developing novel integrated analytical strategies for comprehensively characterizing seaweed similarities based on chemical diversity.
25647718	7	7	theme	analytical	1544:1553	arg1	strategy					1555:1562	this integrated analytical strategy	1528:1562	this integrated analytical strategy	1528:1562	These results suggest that this integrated analytical strategy is useful for extracting and identifying the chemical characteristics of diverse seaweeds based on large chemical data sets, particularly complicated overlapping spectral data.
25647718	1	8	theme	major	191:195	arg1	information					129:139	Extracting useful information	111:139	Extracting useful information from high dimensionality and large data sets	111:184	Extracting useful information from high dimensionality and large data sets is a major challenge for data-driven approaches.
25647718	1	8	theme	major	191:195	arg1	challenge					197:205	a major challenge	189:205	a major challenge for data-driven approaches	189:232	Extracting useful information from high dimensionality and large data sets is a major challenge for data-driven approaches.
25647718	3	9	theme	ratio	798:802	arg1	spectrometry					809:820	isotope ratio mass spectrometry	790:820	isotope ratio mass spectrometry (IR-MS)	790:828	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	3	9	theme	ratio	798:802	arg1	IR-MS					823:827	IR-MS	823:827	IR-MS	823:827	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	3	9	theme	ratio	798:802	arg1	spectroscopy					614:625	infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy	534:625	infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy	534:625	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	5	10	theme	data	1162:1165	arg1	analysis					1124:1131	Integrated analysis	1113:1131	Integrated analysis of the preprocessed chemical data	1113:1165	Integrated analysis of the preprocessed chemical data demonstrated distinct discrimination of differential seaweed species.
25647718	3	11	theme	NMR	609:611	arg1	spectrometry					809:820	isotope ratio mass spectrometry	790:820	isotope ratio mass spectrometry (IR-MS)	790:828	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	3	11	theme	NMR	609:611	arg1	analysis					666:673	thermogravimetry-differential thermal analysis	628:673	thermogravimetry-differential thermal analysis (TG-DTA)	628:682	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	3	11	theme	NMR	609:611	arg1	spectrometry					729:740	plasma-optical emission spectrometry	705:740	plasma-optical emission spectrometry (ICP-OES)	705:750	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	3	11	theme	NMR	609:611	arg1	analysis					776:783	CHNS/O total elemental analysis	753:783	CHNS/O total elemental analysis	753:783	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	3	11	theme	NMR	609:611	arg1	spectroscopy					614:625	infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy	534:625	infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy	534:625	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	5	12	theme	distinct	1180:1187	arg1	discrimination					1189:1202	distinct discrimination	1180:1202	distinct discrimination of differential seaweed species	1180:1234	Integrated analysis of the preprocessed chemical data demonstrated distinct discrimination of differential seaweed species.
25647718	2	13	theme	present	239:245	arg1	study					247:251	The present study	235:251	The present study	235:251	The present study was aimed at developing novel integrated analytical strategies for comprehensively characterizing seaweed similarities based on chemical diversity.
25647718	4	14	theme	resolution-alternating	954:975	arg1	methods					1001:1007	multivariate curve resolution-alternating least-squares (MCR-ALS) methods	935:1007	multivariate curve resolution-alternating least-squares (MCR-ALS) methods	935:1007	The spectral data were preprocessed using non-negative matrix factorization (NMF) and NMF combined with multivariate curve resolution-alternating least-squares (MCR-ALS) methods in order to separate individual component information from the overlapping and/or broad spectral peaks.
25647718	6	15	theme	brown	1357:1361	arg1	algae					1363:1367	brown algae	1357:1367	brown algae	1357:1367	Further network analysis revealed a close correlation between the heavy metal elements and characteristic components of brown algae, such as cellulose, alginic acid, and sulfated mucopolysaccharides, providing a componential basis for its metal-sorbing potential.
25647718	3	16	theme	thermogravimetry-differential	628:656	arg1	TG-DTA					676:681	TG-DTA	676:681	TG-DTA	676:681	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	3	16	theme	thermogravimetry-differential	628:656	arg1	analysis					666:673	thermogravimetry-differential thermal analysis	628:673	thermogravimetry-differential thermal analysis (TG-DTA)	628:682	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	3	16	theme	thermogravimetry-differential	628:656	arg1	spectroscopy					614:625	infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy	534:625	infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy	534:625	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	6	17	theme	sulfated	1407:1414	arg1	mucopolysaccharides					1416:1434	sulfated mucopolysaccharides	1407:1434	sulfated mucopolysaccharides	1407:1434	Further network analysis revealed a close correlation between the heavy metal elements and characteristic components of brown algae, such as cellulose, alginic acid, and sulfated mucopolysaccharides, providing a componential basis for its metal-sorbing potential.
25647718	5	18	theme	preprocessed	1140:1151	arg1	data					1162:1165	the preprocessed chemical data	1136:1165	the preprocessed chemical data	1136:1165	Integrated analysis of the preprocessed chemical data demonstrated distinct discrimination of differential seaweed species.
25647718	1	19	theme	high	146:149	arg1	dimensionality					151:164	high dimensionality	146:164	high dimensionality	146:164	Extracting useful information from high dimensionality and large data sets is a major challenge for data-driven approaches.
25647718	0	20	theme	seaweed	61:67	arg1	similarities					69:80	seaweed similarities	61:80	seaweed similarities based on chemical diversity	61:108	Pretreatment and integrated analysis of spectral data reveal seaweed similarities based on chemical diversity.
25647718	1	21	from	sets	181:184	arg1	information					129:139	Extracting useful information	111:139	Extracting useful information from high dimensionality and large data sets	111:184	Extracting useful information from high dimensionality and large data sets is a major challenge for data-driven approaches.
25647718	1	21	from	sets	181:184	arg1	challenge					197:205	a major challenge	189:205	a major challenge for data-driven approaches	189:232	Extracting useful information from high dimensionality and large data sets is a major challenge for data-driven approaches.
25647718	1	22	theme	data	176:179	arg1	sets					181:184	large data sets	170:184	large data sets	170:184	Extracting useful information from high dimensionality and large data sets is a major challenge for data-driven approaches.
25647718	0	23	theme	chemical	91:98	arg1	diversity					100:108	chemical diversity	91:108	chemical diversity	91:108	Pretreatment and integrated analysis of spectral data reveal seaweed similarities based on chemical diversity.
25647718	2	24	theme	analytical	294:303	arg1	strategies					305:314	novel integrated analytical strategies	277:314	novel integrated analytical strategies for comprehensively characterizing seaweed similarities based on chemical diversity	277:398	The present study was aimed at developing novel integrated analytical strategies for comprehensively characterizing seaweed similarities based on chemical diversity.
25647718	1	25	from	dimensionality	151:164	arg1	information					129:139	Extracting useful information	111:139	Extracting useful information from high dimensionality and large data sets	111:184	Extracting useful information from high dimensionality and large data sets is a major challenge for data-driven approaches.
25647718	1	25	from	dimensionality	151:164	arg1	challenge					197:205	a major challenge	189:205	a major challenge for data-driven approaches	189:232	Extracting useful information from high dimensionality and large data sets is a major challenge for data-driven approaches.
25647718	3	26	dep	infrared	534:541	arg1	FT-IR					544:548	FT-IR	544:548	FT-IR	544:548	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	4	27	theme	individual	1030:1039	arg1	information					1051:1061	separate individual component information	1021:1061	separate individual component information from the overlapping and/or broad spectral peaks	1021:1110	The spectral data were preprocessed using non-negative matrix factorization (NMF) and NMF combined with multivariate curve resolution-alternating least-squares (MCR-ALS) methods in order to separate individual component information from the overlapping and/or broad spectral peaks.
25647718	5	28	theme	seaweed	1220:1226	arg1	species					1228:1234	differential seaweed species	1207:1234	differential seaweed species	1207:1234	Integrated analysis of the preprocessed chemical data demonstrated distinct discrimination of differential seaweed species.
25647718	0	29	theme	integrated	17:26	arg1	analysis					28:35	integrated analysis	17:35	integrated analysis of spectral data	17:52	Pretreatment and integrated analysis of spectral data reveal seaweed similarities based on chemical diversity.
25647718	1	30	theme	Extracting	111:120	arg1	information					129:139	Extracting useful information	111:139	Extracting useful information from high dimensionality and large data sets	111:184	Extracting useful information from high dimensionality and large data sets is a major challenge for data-driven approaches.
25647718	1	30	theme	Extracting	111:120	arg1	challenge					197:205	a major challenge	189:205	a major challenge for data-driven approaches	189:232	Extracting useful information from high dimensionality and large data sets is a major challenge for data-driven approaches.
25647718	3	31	theme	seagrass	448:455	arg1	samples					457:463	107 seaweed and 2 seagrass samples	430:463	samples	457:463	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	3	32	theme	emission	720:727	arg1	ICP-OES					743:749	ICP-OES	743:749	ICP-OES	743:749	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	3	32	theme	emission	720:727	arg1	spectrometry					729:740	plasma-optical emission spectrometry	705:740	plasma-optical emission spectrometry (ICP-OES)	705:750	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	3	32	theme	emission	720:727	arg1	spectroscopy					614:625	infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy	534:625	infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy	534:625	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	4	33	theme	non-negative	873:884	arg1	NMF					908:910	NMF	908:910	NMF	908:910	The spectral data were preprocessed using non-negative matrix factorization (NMF) and NMF combined with multivariate curve resolution-alternating least-squares (MCR-ALS) methods in order to separate individual component information from the overlapping and/or broad spectral peaks.
25647718	4	33	theme	non-negative	873:884	arg1	factorization					893:905	non-negative matrix factorization	873:905	non-negative matrix factorization (NMF)	873:911	The spectral data were preprocessed using non-negative matrix factorization (NMF) and NMF combined with multivariate curve resolution-alternating least-squares (MCR-ALS) methods in order to separate individual component information from the overlapping and/or broad spectral peaks.
25647718	4	34	theme	overlapping	1072:1082	arg1	peaks					1106:1110	the overlapping and/or broad spectral peaks	1068:1110	the overlapping and/or broad spectral peaks	1068:1110	The spectral data were preprocessed using non-negative matrix factorization (NMF) and NMF combined with multivariate curve resolution-alternating least-squares (MCR-ALS) methods in order to separate individual component information from the overlapping and/or broad spectral peaks.
25647718	7	35	theme	data	1678:1681	arg1	sets					1683:1686	large chemical data sets	1663:1686	large chemical data sets	1663:1686	These results suggest that this integrated analytical strategy is useful for extracting and identifying the chemical characteristics of diverse seaweeds based on large chemical data sets, particularly complicated overlapping spectral data.
25647718	4	36	theme	spectral	1097:1104	arg1	peaks					1106:1110	the overlapping and/or broad spectral peaks	1068:1110	the overlapping and/or broad spectral peaks	1068:1110	The spectral data were preprocessed using non-negative matrix factorization (NMF) and NMF combined with multivariate curve resolution-alternating least-squares (MCR-ALS) methods in order to separate individual component information from the overlapping and/or broad spectral peaks.
25647718	3	37	theme	coupled	697:703	arg1	ICP-OES					743:749	ICP-OES	743:749	ICP-OES	743:749	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	3	37	theme	coupled	697:703	arg1	spectrometry					729:740	plasma-optical emission spectrometry	705:740	plasma-optical emission spectrometry (ICP-OES)	705:750	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	3	37	theme	coupled	697:703	arg1	spectroscopy					614:625	infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy	534:625	infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy	534:625	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	2	38	theme	chemical	381:388	arg1	diversity					390:398	chemical diversity	381:398	chemical diversity	381:398	The present study was aimed at developing novel integrated analytical strategies for comprehensively characterizing seaweed similarities based on chemical diversity.
25647718	0	39	theme	data	49:52	arg1	Pretreatment					0:11	Pretreatment	0:11	Pretreatment	0:11	Pretreatment and integrated analysis of spectral data reveal seaweed similarities based on chemical diversity.
25647718	0	39	theme	data	49:52	arg1	analysis					28:35	integrated analysis	17:35	integrated analysis of spectral data	17:52	Pretreatment and integrated analysis of spectral data reveal seaweed similarities based on chemical diversity.
25647718	7	40	theme	large	1663:1667	arg1	sets					1683:1686	large chemical data sets	1663:1686	large chemical data sets	1663:1686	These results suggest that this integrated analytical strategy is useful for extracting and identifying the chemical characteristics of diverse seaweeds based on large chemical data sets, particularly complicated overlapping spectral data.
25647718	6	41	theme	network	1245:1251	arg1	analysis					1253:1260	Further network analysis	1237:1260	Further network analysis	1237:1260	Further network analysis revealed a close correlation between the heavy metal elements and characteristic components of brown algae, such as cellulose, alginic acid, and sulfated mucopolysaccharides, providing a componential basis for its metal-sorbing potential.
25647718	3	42	theme	nuclear	581:587	arg1	spectrometry					809:820	isotope ratio mass spectrometry	790:820	isotope ratio mass spectrometry (IR-MS)	790:828	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	3	42	theme	nuclear	581:587	arg1	analysis					666:673	thermogravimetry-differential thermal analysis	628:673	thermogravimetry-differential thermal analysis (TG-DTA)	628:682	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	3	42	theme	nuclear	581:587	arg1	spectrometry					729:740	plasma-optical emission spectrometry	705:740	plasma-optical emission spectrometry (ICP-OES)	705:750	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	3	42	theme	nuclear	581:587	arg1	analysis					776:783	CHNS/O total elemental analysis	753:783	CHNS/O total elemental analysis	753:783	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	3	42	theme	nuclear	581:587	arg1	spectroscopy					614:625	infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy	534:625	infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy	534:625	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	3	43	theme	solid-	555:560	arg1	spectrometry					809:820	isotope ratio mass spectrometry	790:820	isotope ratio mass spectrometry (IR-MS)	790:828	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	3	43	theme	solid-	555:560	arg1	analysis					666:673	thermogravimetry-differential thermal analysis	628:673	thermogravimetry-differential thermal analysis (TG-DTA)	628:682	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	3	43	theme	solid-	555:560	arg1	spectrometry					729:740	plasma-optical emission spectrometry	705:740	plasma-optical emission spectrometry (ICP-OES)	705:750	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	3	43	theme	solid-	555:560	arg1	analysis					776:783	CHNS/O total elemental analysis	753:783	CHNS/O total elemental analysis	753:783	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	3	43	theme	solid-	555:560	arg1	spectroscopy					614:625	infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy	534:625	infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy	534:625	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	7	44	theme	diverse	1637:1643	arg1	seaweeds					1645:1652	diverse seaweeds	1637:1652	diverse seaweeds based on large chemical data sets, particularly complicated overlapping spectral data	1637:1738	These results suggest that this integrated analytical strategy is useful for extracting and identifying the chemical characteristics of diverse seaweeds based on large chemical data sets, particularly complicated overlapping spectral data.
25647718	3	45	dep	Fourier	516:522	arg1	transform					524:532	transform	524:532	transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS)	524:828	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	3	46	theme	elemental	766:774	arg1	analysis					776:783	CHNS/O total elemental analysis	753:783	CHNS/O total elemental analysis	753:783	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	3	46	theme	elemental	766:774	arg1	spectroscopy					614:625	infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy	534:625	infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy	534:625	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	3	47	theme	CHNS/O	753:758	arg1	analysis					776:783	CHNS/O total elemental analysis	753:783	CHNS/O total elemental analysis	753:783	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	3	47	theme	CHNS/O	753:758	arg1	spectroscopy					614:625	infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy	534:625	infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy	534:625	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	3	48	theme	magnetic	589:596	arg1	spectrometry					809:820	isotope ratio mass spectrometry	790:820	isotope ratio mass spectrometry (IR-MS)	790:828	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	3	48	theme	magnetic	589:596	arg1	analysis					666:673	thermogravimetry-differential thermal analysis	628:673	thermogravimetry-differential thermal analysis (TG-DTA)	628:682	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	3	48	theme	magnetic	589:596	arg1	spectrometry					729:740	plasma-optical emission spectrometry	705:740	plasma-optical emission spectrometry (ICP-OES)	705:750	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	3	48	theme	magnetic	589:596	arg1	analysis					776:783	CHNS/O total elemental analysis	753:783	CHNS/O total elemental analysis	753:783	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	3	48	theme	magnetic	589:596	arg1	spectroscopy					614:625	infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy	534:625	infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy	534:625	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	6	49	theme	heavy	1303:1307	arg1	cellulose					1378:1386	cellulose	1378:1386	cellulose	1378:1386	Further network analysis revealed a close correlation between the heavy metal elements and characteristic components of brown algae, such as cellulose, alginic acid, and sulfated mucopolysaccharides, providing a componential basis for its metal-sorbing potential.
25647718	6	49	theme	heavy	1303:1307	arg1	acid					1397:1400	alginic acid	1389:1400	alginic acid	1389:1400	Further network analysis revealed a close correlation between the heavy metal elements and characteristic components of brown algae, such as cellulose, alginic acid, and sulfated mucopolysaccharides, providing a componential basis for its metal-sorbing potential.
25647718	6	49	theme	heavy	1303:1307	arg1	elements					1315:1322	heavy metal elements	1303:1322	heavy metal elements	1303:1322	Further network analysis revealed a close correlation between the heavy metal elements and characteristic components of brown algae, such as cellulose, alginic acid, and sulfated mucopolysaccharides, providing a componential basis for its metal-sorbing potential.
25647718	6	49	theme	heavy	1303:1307	arg1	mucopolysaccharides					1416:1434	sulfated mucopolysaccharides	1407:1434	sulfated mucopolysaccharides	1407:1434	Further network analysis revealed a close correlation between the heavy metal elements and characteristic components of brown algae, such as cellulose, alginic acid, and sulfated mucopolysaccharides, providing a componential basis for its metal-sorbing potential.
25647718	3	50	theme	isotope	790:796	arg1	spectrometry					809:820	isotope ratio mass spectrometry	790:820	isotope ratio mass spectrometry (IR-MS)	790:828	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	3	50	theme	isotope	790:796	arg1	IR-MS					823:827	IR-MS	823:827	IR-MS	823:827	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	3	50	theme	isotope	790:796	arg1	spectroscopy					614:625	infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy	534:625	infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy	534:625	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	2	51	theme	integrated	283:292	arg1	strategies					305:314	novel integrated analytical strategies	277:314	novel integrated analytical strategies for comprehensively characterizing seaweed similarities based on chemical diversity	277:398	The present study was aimed at developing novel integrated analytical strategies for comprehensively characterizing seaweed similarities based on chemical diversity.
25647718	2	52	theme	seaweed	351:357	arg1	similarities					359:370	seaweed similarities	351:370	seaweed similarities based on chemical diversity	351:398	The present study was aimed at developing novel integrated analytical strategies for comprehensively characterizing seaweed similarities based on chemical diversity.
25647718	6	53	theme	close	1273:1277	arg1	correlation					1279:1289	a close correlation	1271:1289	a close correlation between the heavy metal elements and characteristic components of brown algae, such as cellulose, alginic acid, and sulfated mucopolysaccharides	1271:1434	Further network analysis revealed a close correlation between the heavy metal elements and characteristic components of brown algae, such as cellulose, alginic acid, and sulfated mucopolysaccharides, providing a componential basis for its metal-sorbing potential.
25647718	4	54	theme	MCR-ALS	992:998	arg1	methods					1001:1007	multivariate curve resolution-alternating least-squares (MCR-ALS) methods	935:1007	multivariate curve resolution-alternating least-squares (MCR-ALS) methods	935:1007	The spectral data were preprocessed using non-negative matrix factorization (NMF) and NMF combined with multivariate curve resolution-alternating least-squares (MCR-ALS) methods in order to separate individual component information from the overlapping and/or broad spectral peaks.
25647718	7	55	theme	integrated	1533:1542	arg1	strategy					1555:1562	this integrated analytical strategy	1528:1562	this integrated analytical strategy	1528:1562	These results suggest that this integrated analytical strategy is useful for extracting and identifying the chemical characteristics of diverse seaweeds based on large chemical data sets, particularly complicated overlapping spectral data.
25647718	3	56	theme	mass	804:807	arg1	spectrometry					809:820	isotope ratio mass spectrometry	790:820	isotope ratio mass spectrometry (IR-MS)	790:828	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	3	56	theme	mass	804:807	arg1	IR-MS					823:827	IR-MS	823:827	IR-MS	823:827	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	3	56	theme	mass	804:807	arg1	spectroscopy					614:625	infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy	534:625	infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy	534:625	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	6	57	theme	componential	1449:1460	arg1	basis					1462:1466	a componential basis	1447:1466	a componential basis for its metal-sorbing potential	1447:1498	Further network analysis revealed a close correlation between the heavy metal elements and characteristic components of brown algae, such as cellulose, alginic acid, and sulfated mucopolysaccharides, providing a componential basis for its metal-sorbing potential.
25647718	3	58	theme	thermal	658:664	arg1	TG-DTA					676:681	TG-DTA	676:681	TG-DTA	676:681	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	3	58	theme	thermal	658:664	arg1	analysis					666:673	thermogravimetry-differential thermal analysis	628:673	thermogravimetry-differential thermal analysis (TG-DTA)	628:682	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	3	58	theme	thermal	658:664	arg1	spectroscopy					614:625	infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy	534:625	infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy	534:625	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	1	59	theme	data-driven	211:221	arg1	approaches					223:232	data-driven approaches	211:232	data-driven approaches	211:232	Extracting useful information from high dimensionality and large data sets is a major challenge for data-driven approaches.
25647718	6	60	theme	algae	1363:1367	arg1	cellulose					1378:1386	cellulose	1378:1386	cellulose	1378:1386	Further network analysis revealed a close correlation between the heavy metal elements and characteristic components of brown algae, such as cellulose, alginic acid, and sulfated mucopolysaccharides, providing a componential basis for its metal-sorbing potential.
25647718	6	60	theme	algae	1363:1367	arg1	acid					1397:1400	alginic acid	1389:1400	alginic acid	1389:1400	Further network analysis revealed a close correlation between the heavy metal elements and characteristic components of brown algae, such as cellulose, alginic acid, and sulfated mucopolysaccharides, providing a componential basis for its metal-sorbing potential.
25647718	6	60	theme	algae	1363:1367	arg1	elements					1315:1322	heavy metal elements	1303:1322	heavy metal elements	1303:1322	Further network analysis revealed a close correlation between the heavy metal elements and characteristic components of brown algae, such as cellulose, alginic acid, and sulfated mucopolysaccharides, providing a componential basis for its metal-sorbing potential.
25647718	6	60	theme	algae	1363:1367	arg1	components					1343:1352	characteristic components	1328:1352	characteristic components	1328:1352	Further network analysis revealed a close correlation between the heavy metal elements and characteristic components of brown algae, such as cellulose, alginic acid, and sulfated mucopolysaccharides, providing a componential basis for its metal-sorbing potential.
25647718	6	60	theme	algae	1363:1367	arg1	mucopolysaccharides					1416:1434	sulfated mucopolysaccharides	1407:1434	sulfated mucopolysaccharides	1407:1434	Further network analysis revealed a close correlation between the heavy metal elements and characteristic components of brown algae, such as cellulose, alginic acid, and sulfated mucopolysaccharides, providing a componential basis for its metal-sorbing potential.
25647718	1	61	theme	useful	122:127	arg1	information					129:139	Extracting useful information	111:139	Extracting useful information from high dimensionality and large data sets	111:184	Extracting useful information from high dimensionality and large data sets is a major challenge for data-driven approaches.
25647718	1	61	theme	useful	122:127	arg1	challenge					197:205	a major challenge	189:205	a major challenge for data-driven approaches	189:232	Extracting useful information from high dimensionality and large data sets is a major challenge for data-driven approaches.
25647718	4	62	theme	curve	948:952	arg1	methods					1001:1007	multivariate curve resolution-alternating least-squares (MCR-ALS) methods	935:1007	multivariate curve resolution-alternating least-squares (MCR-ALS) methods	935:1007	The spectral data were preprocessed using non-negative matrix factorization (NMF) and NMF combined with multivariate curve resolution-alternating least-squares (MCR-ALS) methods in order to separate individual component information from the overlapping and/or broad spectral peaks.
25647718	6	63	dep	elements	1315:1322	arg1	the					1299:1301	the	1299:1301	the	1299:1301	Further network analysis revealed a close correlation between the heavy metal elements and characteristic components of brown algae, such as cellulose, alginic acid, and sulfated mucopolysaccharides, providing a componential basis for its metal-sorbing potential.
25647718	4	64	theme	least-squares	977:989	arg1	methods					1001:1007	multivariate curve resolution-alternating least-squares (MCR-ALS) methods	935:1007	multivariate curve resolution-alternating least-squares (MCR-ALS) methods	935:1007	The spectral data were preprocessed using non-negative matrix factorization (NMF) and NMF combined with multivariate curve resolution-alternating least-squares (MCR-ALS) methods in order to separate individual component information from the overlapping and/or broad spectral peaks.
25647718	6	65	theme	characteristic	1328:1341	arg1	cellulose					1378:1386	cellulose	1378:1386	cellulose	1378:1386	Further network analysis revealed a close correlation between the heavy metal elements and characteristic components of brown algae, such as cellulose, alginic acid, and sulfated mucopolysaccharides, providing a componential basis for its metal-sorbing potential.
25647718	6	65	theme	characteristic	1328:1341	arg1	acid					1397:1400	alginic acid	1389:1400	alginic acid	1389:1400	Further network analysis revealed a close correlation between the heavy metal elements and characteristic components of brown algae, such as cellulose, alginic acid, and sulfated mucopolysaccharides, providing a componential basis for its metal-sorbing potential.
25647718	6	65	theme	characteristic	1328:1341	arg1	components					1343:1352	characteristic components	1328:1352	characteristic components	1328:1352	Further network analysis revealed a close correlation between the heavy metal elements and characteristic components of brown algae, such as cellulose, alginic acid, and sulfated mucopolysaccharides, providing a componential basis for its metal-sorbing potential.
25647718	6	65	theme	characteristic	1328:1341	arg1	mucopolysaccharides					1416:1434	sulfated mucopolysaccharides	1407:1434	sulfated mucopolysaccharides	1407:1434	Further network analysis revealed a close correlation between the heavy metal elements and characteristic components of brown algae, such as cellulose, alginic acid, and sulfated mucopolysaccharides, providing a componential basis for its metal-sorbing potential.
25647718	5	66	theme	chemical	1153:1160	arg1	data					1162:1165	the preprocessed chemical data	1136:1165	the preprocessed chemical data	1136:1165	Integrated analysis of the preprocessed chemical data demonstrated distinct discrimination of differential seaweed species.
25647718	4	67	theme	component	1041:1049	arg1	information					1051:1061	separate individual component information	1021:1061	separate individual component information from the overlapping and/or broad spectral peaks	1021:1110	The spectral data were preprocessed using non-negative matrix factorization (NMF) and NMF combined with multivariate curve resolution-alternating least-squares (MCR-ALS) methods in order to separate individual component information from the overlapping and/or broad spectral peaks.
25647718	1	68	theme	large	170:174	arg1	sets					181:184	large data sets	170:184	large data sets	170:184	Extracting useful information from high dimensionality and large data sets is a major challenge for data-driven approaches.
25647718	7	69	theme	spectral	1726:1733	arg1	data					1735:1738	spectral data	1726:1738	spectral data	1726:1738	These results suggest that this integrated analytical strategy is useful for extracting and identifying the chemical characteristics of diverse seaweeds based on large chemical data sets, particularly complicated overlapping spectral data.
25647718	3	70	theme	chemical	405:412	arg1	compositions					414:425	The chemical compositions	401:425	The chemical compositions of 107 seaweed and 2 seagrass samples	401:463	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	6	71	theme	metal-sorbing	1476:1488	arg1	potential					1490:1498	its metal-sorbing potential	1472:1498	its metal-sorbing potential	1472:1498	Further network analysis revealed a close correlation between the heavy metal elements and characteristic components of brown algae, such as cellulose, alginic acid, and sulfated mucopolysaccharides, providing a componential basis for its metal-sorbing potential.
25647718	5	72	theme	differential	1207:1218	arg1	species					1228:1234	differential seaweed species	1207:1234	differential seaweed species	1207:1234	Integrated analysis of the preprocessed chemical data demonstrated distinct discrimination of differential seaweed species.
25647718	4	73	theme	separate	1021:1028	arg1	information					1051:1061	separate individual component information	1021:1061	separate individual component information from the overlapping and/or broad spectral peaks	1021:1110	The spectral data were preprocessed using non-negative matrix factorization (NMF) and NMF combined with multivariate curve resolution-alternating least-squares (MCR-ALS) methods in order to separate individual component information from the overlapping and/or broad spectral peaks.
25647718	3	74	theme	multiple	485:492	arg1	techniques					494:503	multiple techniques	485:503	multiple techniques	485:503	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	3	74	theme	multiple	485:492	arg1	Fourier					516:522	Fourier	516:522	Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS)	516:828	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	5	75	theme	species	1228:1234	arg1	discrimination					1189:1202	distinct discrimination	1180:1202	distinct discrimination of differential seaweed species	1180:1234	Integrated analysis of the preprocessed chemical data demonstrated distinct discrimination of differential seaweed species.
25647718	3	76	theme	seaweed	434:440	arg1	compositions					414:425	The chemical compositions	401:425	The chemical compositions of 107 seaweed and 2 seagrass samples	401:463	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	3	77	theme	plasma-optical	705:718	arg1	ICP-OES					743:749	ICP-OES	743:749	ICP-OES	743:749	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	3	77	theme	plasma-optical	705:718	arg1	spectrometry					729:740	plasma-optical emission spectrometry	705:740	plasma-optical emission spectrometry (ICP-OES)	705:750	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	3	77	theme	plasma-optical	705:718	arg1	spectroscopy					614:625	infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy	534:625	infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy	534:625	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	0	78	theme	spectral	40:47	arg1	data					49:52	spectral data	40:52	spectral data	40:52	Pretreatment and integrated analysis of spectral data reveal seaweed similarities based on chemical diversity.
25647718	4	79	from	peaks	1106:1110	arg1	information					1051:1061	separate individual component information	1021:1061	separate individual component information from the overlapping and/or broad spectral peaks	1021:1110	The spectral data were preprocessed using non-negative matrix factorization (NMF) and NMF combined with multivariate curve resolution-alternating least-squares (MCR-ALS) methods in order to separate individual component information from the overlapping and/or broad spectral peaks.
25647718	3	80	theme	samples	457:463	arg1	compositions					414:425	The chemical compositions	401:425	The chemical compositions of 107 seaweed and 2 seagrass samples	401:463	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	5	81	theme	Integrated	1113:1122	arg1	analysis					1124:1131	Integrated analysis	1113:1131	Integrated analysis of the preprocessed chemical data	1113:1165	Integrated analysis of the preprocessed chemical data demonstrated distinct discrimination of differential seaweed species.
25647718	4	82	theme	broad	1091:1095	arg1	peaks					1106:1110	the overlapping and/or broad spectral peaks	1068:1110	the overlapping and/or broad spectral peaks	1068:1110	The spectral data were preprocessed using non-negative matrix factorization (NMF) and NMF combined with multivariate curve resolution-alternating least-squares (MCR-ALS) methods in order to separate individual component information from the overlapping and/or broad spectral peaks.
25647718	4	83	theme	matrix	886:891	arg1	NMF					908:910	NMF	908:910	NMF	908:910	The spectral data were preprocessed using non-negative matrix factorization (NMF) and NMF combined with multivariate curve resolution-alternating least-squares (MCR-ALS) methods in order to separate individual component information from the overlapping and/or broad spectral peaks.
25647718	4	83	theme	matrix	886:891	arg1	factorization					893:905	non-negative matrix factorization	873:905	non-negative matrix factorization (NMF)	873:911	The spectral data were preprocessed using non-negative matrix factorization (NMF) and NMF combined with multivariate curve resolution-alternating least-squares (MCR-ALS) methods in order to separate individual component information from the overlapping and/or broad spectral peaks.
25647718	7	84	theme	chemical	1669:1676	arg1	sets					1683:1686	large chemical data sets	1663:1686	large chemical data sets	1663:1686	These results suggest that this integrated analytical strategy is useful for extracting and identifying the chemical characteristics of diverse seaweeds based on large chemical data sets, particularly complicated overlapping spectral data.
25647718	6	85	theme	Further	1237:1243	arg1	analysis					1253:1260	Further network analysis	1237:1260	Further network analysis	1237:1260	Further network analysis revealed a close correlation between the heavy metal elements and characteristic components of brown algae, such as cellulose, alginic acid, and sulfated mucopolysaccharides, providing a componential basis for its metal-sorbing potential.
25647718	4	86	theme	spectral	835:842	arg1	data					844:847	The spectral data	831:847	The spectral data	831:847	The spectral data were preprocessed using non-negative matrix factorization (NMF) and NMF combined with multivariate curve resolution-alternating least-squares (MCR-ALS) methods in order to separate individual component information from the overlapping and/or broad spectral peaks.
25647718	3	87	theme	infrared	534:541	arg1	spectrometry					809:820	isotope ratio mass spectrometry	790:820	isotope ratio mass spectrometry (IR-MS)	790:828	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	3	87	theme	infrared	534:541	arg1	analysis					666:673	thermogravimetry-differential thermal analysis	628:673	thermogravimetry-differential thermal analysis (TG-DTA)	628:682	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	3	87	theme	infrared	534:541	arg1	spectrometry					729:740	plasma-optical emission spectrometry	705:740	plasma-optical emission spectrometry (ICP-OES)	705:750	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	3	87	theme	infrared	534:541	arg1	analysis					776:783	CHNS/O total elemental analysis	753:783	CHNS/O total elemental analysis	753:783	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	3	87	theme	infrared	534:541	arg1	spectroscopy					614:625	infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy	534:625	infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy	534:625	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	3	88	theme	total	760:764	arg1	analysis					776:783	CHNS/O total elemental analysis	753:783	CHNS/O total elemental analysis	753:783	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
25647718	3	88	theme	total	760:764	arg1	spectroscopy					614:625	infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy	534:625	infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy	534:625	The chemical compositions of 107 seaweed and 2 seagrass samples were analyzed using multiple techniques, including Fourier transform infrared (FT-IR) and solid- and solution-state nuclear magnetic resonance (NMR) spectroscopy, thermogravimetry-differential thermal analysis (TG-DTA), inductively coupled plasma-optical emission spectrometry (ICP-OES), CHNS/O total elemental analysis, and isotope ratio mass spectrometry (IR-MS).
27427666	1	0	theme	metal	175:179	arg1	composition					181:191	the metal composition	171:191	the metal composition of chitosan (CTS) and metal ions	171:224	Metal-Chitosan (CTS) composite was first synthesized through the metal composition of chitosan (CTS) and metal ions.
27427666	5	1	theme	composite	1056:1064	arg1	surface					1020:1026	the surface	1016:1026	the surface of the multilayer thin-film composite	1016:1064	AFM images showed that the surface of the multilayer thin-film composite had some degree of roughness and metal nanoparticles of 10-20 nm in size were generated on the membrane.
27427666	2	2	theme	metal	497:501	arg1	nanoparticles					503:515	metal nanoparticles	497:515	metal nanoparticles	497:515	The formed composite was alternately deposited on the base with sodium polystyrene sulfonate (PSS) through a layer-by-layer self-assembling technique, followed by an in situ reduction by sodium borohydride to produce a polyelectrolyte nanocomposite thin film containing metal nanoparticles.
27427666	2	3	theme	self-assembling	351:365	arg1	technique					367:375	a layer-by-layer self-assembling technique	334:375	a layer-by-layer self-assembling technique	334:375	The formed composite was alternately deposited on the base with sodium polystyrene sulfonate (PSS) through a layer-by-layer self-assembling technique, followed by an in situ reduction by sodium borohydride to produce a polyelectrolyte nanocomposite thin film containing metal nanoparticles.
27427666	5	4	from	nanoparticles	1105:1117	arg1	size					1134:1137	size	1134:1137	size	1134:1137	AFM images showed that the surface of the multilayer thin-film composite had some degree of roughness and metal nanoparticles of 10-20 nm in size were generated on the membrane.
27427666	4	5	theme	linear	891:896	arg1	relationship					898:909	a good linear relationship	884:909	a good linear relationship	884:909	The UV-Vis results indicated that the absorbance of the multilayer film at the characteristic absorption peak increased as the membrane bilayers increased, in a good linear relationship, which demonstrated that the multilayer film was uniformly assembled on the base.
27427666	2	6	theme	sodium	291:296	arg1	sulfonate					310:318	sodium polystyrene sulfonate	291:318	sodium polystyrene sulfonate (PSS)	291:324	The formed composite was alternately deposited on the base with sodium polystyrene sulfonate (PSS) through a layer-by-layer self-assembling technique, followed by an in situ reduction by sodium borohydride to produce a polyelectrolyte nanocomposite thin film containing metal nanoparticles.
27427666	2	6	theme	sodium	291:296	arg1	PSS					321:323	PSS	321:323	PSS	321:323	The formed composite was alternately deposited on the base with sodium polystyrene sulfonate (PSS) through a layer-by-layer self-assembling technique, followed by an in situ reduction by sodium borohydride to produce a polyelectrolyte nanocomposite thin film containing metal nanoparticles.
27427666	3	7	theme	membrane	595:602	arg1	Assembly					518:525	Assembly	518:525	Assembly	518:525	Assembly, surface morphology and electrochemical properties of the composite membrane were analyzed by UV-visible absorption spectroscopy (UV-vis), atomic force microscopy (AFM) and cyclic voltammetry (CV).
27427666	3	7	theme	membrane	595:602	arg1	morphology					536:545	surface morphology	528:545	surface morphology	528:545	Assembly, surface morphology and electrochemical properties of the composite membrane were analyzed by UV-visible absorption spectroscopy (UV-vis), atomic force microscopy (AFM) and cyclic voltammetry (CV).
27427666	3	7	theme	membrane	595:602	arg1	properties					567:576	electrochemical properties	551:576	electrochemical properties	551:576	Assembly, surface morphology and electrochemical properties of the composite membrane were analyzed by UV-visible absorption spectroscopy (UV-vis), atomic force microscopy (AFM) and cyclic voltammetry (CV).
27427666	5	8	theme	metal	1099:1103	arg1	nanoparticles					1105:1117	metal nanoparticles	1099:1117	metal nanoparticles of 10-20 nm in size	1099:1137	AFM images showed that the surface of the multilayer thin-film composite had some degree of roughness and metal nanoparticles of 10-20 nm in size were generated on the membrane.
27427666	4	9	theme	good	886:889	arg1	relationship					898:909	a good linear relationship	884:909	a good linear relationship	884:909	The UV-Vis results indicated that the absorbance of the multilayer film at the characteristic absorption peak increased as the membrane bilayers increased, in a good linear relationship, which demonstrated that the multilayer film was uniformly assembled on the base.
27427666	6	10	theme	CV	1175:1176	arg1	results					1178:1184	The CV results	1171:1184	The CV results	1171:1184	The CV results indicated that the metal nanocomposite film had excellent electrocatalytic activity to glucose and had a potential for applications in electrochemical sensors.
27427666	3	11	theme	electrochemical	551:565	arg1	properties					567:576	electrochemical properties	551:576	electrochemical properties	551:576	Assembly, surface morphology and electrochemical properties of the composite membrane were analyzed by UV-visible absorption spectroscopy (UV-vis), atomic force microscopy (AFM) and cyclic voltammetry (CV).
27427666	6	12	contain	had	1285:1287	arg2	potential					1291:1299	a potential	1289:1299	a potential for applications in electrochemical sensors	1289:1343	The CV results indicated that the metal nanocomposite film had excellent electrocatalytic activity to glucose and had a potential for applications in electrochemical sensors.
27427666	6	12	contain	had	1285:1287	arg1	film					1225:1228	the metal nanocomposite film	1201:1228	the metal nanocomposite film	1201:1228	The CV results indicated that the metal nanocomposite film had excellent electrocatalytic activity to glucose and had a potential for applications in electrochemical sensors.
27427666	2	13	theme	nanocomposite	462:474	arg1	film					481:484	a polyelectrolyte nanocomposite thin film	444:484	a polyelectrolyte nanocomposite thin film containing metal nanoparticles	444:515	The formed composite was alternately deposited on the base with sodium polystyrene sulfonate (PSS) through a layer-by-layer self-assembling technique, followed by an in situ reduction by sodium borohydride to produce a polyelectrolyte nanocomposite thin film containing metal nanoparticles.
27427666	4	14	theme	membrane	852:859	arg1	bilayers					861:868	the membrane bilayers	848:868	the membrane bilayers	848:868	The UV-Vis results indicated that the absorbance of the multilayer film at the characteristic absorption peak increased as the membrane bilayers increased, in a good linear relationship, which demonstrated that the multilayer film was uniformly assembled on the base.
27427666	4	15	theme	multilayer	781:790	arg1	film					792:795	the multilayer film	777:795	the multilayer film	777:795	The UV-Vis results indicated that the absorbance of the multilayer film at the characteristic absorption peak increased as the membrane bilayers increased, in a good linear relationship, which demonstrated that the multilayer film was uniformly assembled on the base.
27427666	1	16	theme	chitosan	196:203	arg1	composition					181:191	the metal composition	171:191	the metal composition of chitosan (CTS) and metal ions	171:224	Metal-Chitosan (CTS) composite was first synthesized through the metal composition of chitosan (CTS) and metal ions.
27427666	2	17	theme	polyelectrolyte	446:460	arg1	film					481:484	a polyelectrolyte nanocomposite thin film	444:484	a polyelectrolyte nanocomposite thin film containing metal nanoparticles	444:515	The formed composite was alternately deposited on the base with sodium polystyrene sulfonate (PSS) through a layer-by-layer self-assembling technique, followed by an in situ reduction by sodium borohydride to produce a polyelectrolyte nanocomposite thin film containing metal nanoparticles.
27427666	1	18	theme	Metal-Chitosan	110:123	arg1	composite					131:139	Metal-Chitosan (CTS) composite	110:139	Metal-Chitosan (CTS) composite	110:139	Metal-Chitosan (CTS) composite was first synthesized through the metal composition of chitosan (CTS) and metal ions.
27427666	6	19	theme	electrocatalytic	1244:1259	arg1	activity					1261:1268	excellent electrocatalytic activity	1234:1268	excellent electrocatalytic activity to glucose	1234:1279	The CV results indicated that the metal nanocomposite film had excellent electrocatalytic activity to glucose and had a potential for applications in electrochemical sensors.
27427666	5	20	theme	thin-film	1046:1054	arg1	composite					1056:1064	the multilayer thin-film composite	1031:1064	the multilayer thin-film composite	1031:1064	AFM images showed that the surface of the multilayer thin-film composite had some degree of roughness and metal nanoparticles of 10-20 nm in size were generated on the membrane.
27427666	2	21	dep	deposited	264:272	arg1	followed					378:385	followed	378:385	followed by an in situ reduction by sodium borohydride to produce a polyelectrolyte nanocomposite thin film containing metal nanoparticles	378:515	The formed composite was alternately deposited on the base with sodium polystyrene sulfonate (PSS) through a layer-by-layer self-assembling technique, followed by an in situ reduction by sodium borohydride to produce a polyelectrolyte nanocomposite thin film containing metal nanoparticles.
27427666	0	22	theme	Sulfonate	36:44	arg1	Nanoparticles					75:87	Chitosan/Polystyrene Sulfonate Multilayered Composite Metal Nanoparticles	15:87	Chitosan/Polystyrene Sulfonate Multilayered Composite Metal Nanoparticles	15:87	Preparation of Chitosan/Polystyrene Sulfonate Multilayered Composite Metal Nanoparticles and Its Application.
27427666	2	23	contain	containing	486:495	arg1	film					481:484	a polyelectrolyte nanocomposite thin film	444:484	a polyelectrolyte nanocomposite thin film containing metal nanoparticles	444:515	The formed composite was alternately deposited on the base with sodium polystyrene sulfonate (PSS) through a layer-by-layer self-assembling technique, followed by an in situ reduction by sodium borohydride to produce a polyelectrolyte nanocomposite thin film containing metal nanoparticles.
27427666	2	23	contain	containing	486:495	arg2	nanoparticles					503:515	metal nanoparticles	497:515	metal nanoparticles	497:515	The formed composite was alternately deposited on the base with sodium polystyrene sulfonate (PSS) through a layer-by-layer self-assembling technique, followed by an in situ reduction by sodium borohydride to produce a polyelectrolyte nanocomposite thin film containing metal nanoparticles.
27427666	1	24	theme	CTS	126:128	arg1	composite					131:139	Metal-Chitosan (CTS) composite	110:139	Metal-Chitosan (CTS) composite	110:139	Metal-Chitosan (CTS) composite was first synthesized through the metal composition of chitosan (CTS) and metal ions.
27427666	6	25	theme	excellent	1234:1242	arg1	activity					1261:1268	excellent electrocatalytic activity	1234:1268	excellent electrocatalytic activity to glucose	1234:1279	The CV results indicated that the metal nanocomposite film had excellent electrocatalytic activity to glucose and had a potential for applications in electrochemical sensors.
27427666	0	26	theme	Chitosan/Polystyrene	15:34	arg1	Nanoparticles					75:87	Chitosan/Polystyrene Sulfonate Multilayered Composite Metal Nanoparticles	15:87	Chitosan/Polystyrene Sulfonate Multilayered Composite Metal Nanoparticles	15:87	Preparation of Chitosan/Polystyrene Sulfonate Multilayered Composite Metal Nanoparticles and Its Application.
27427666	6	27	contain	had	1230:1232	arg1	film					1225:1228	the metal nanocomposite film	1201:1228	the metal nanocomposite film	1201:1228	The CV results indicated that the metal nanocomposite film had excellent electrocatalytic activity to glucose and had a potential for applications in electrochemical sensors.
27427666	6	27	contain	had	1230:1232	arg2	activity					1261:1268	excellent electrocatalytic activity	1234:1268	excellent electrocatalytic activity to glucose	1234:1279	The CV results indicated that the metal nanocomposite film had excellent electrocatalytic activity to glucose and had a potential for applications in electrochemical sensors.
27427666	4	28	theme	film	792:795	arg1	absorbance					763:772	the absorbance	759:772	the absorbance of the multilayer film at the characteristic absorption peak increased as the membrane bilayers	759:868	The UV-Vis results indicated that the absorbance of the multilayer film at the characteristic absorption peak increased as the membrane bilayers increased, in a good linear relationship, which demonstrated that the multilayer film was uniformly assembled on the base.
27427666	2	29	theme	polystyrene	298:308	arg1	sulfonate					310:318	sodium polystyrene sulfonate	291:318	sodium polystyrene sulfonate (PSS)	291:324	The formed composite was alternately deposited on the base with sodium polystyrene sulfonate (PSS) through a layer-by-layer self-assembling technique, followed by an in situ reduction by sodium borohydride to produce a polyelectrolyte nanocomposite thin film containing metal nanoparticles.
27427666	2	29	theme	polystyrene	298:308	arg1	PSS					321:323	PSS	321:323	PSS	321:323	The formed composite was alternately deposited on the base with sodium polystyrene sulfonate (PSS) through a layer-by-layer self-assembling technique, followed by an in situ reduction by sodium borohydride to produce a polyelectrolyte nanocomposite thin film containing metal nanoparticles.
27427666	2	30	theme	sodium	414:419	arg1	borohydride					421:431	sodium borohydride	414:431	sodium borohydride	414:431	The formed composite was alternately deposited on the base with sodium polystyrene sulfonate (PSS) through a layer-by-layer self-assembling technique, followed by an in situ reduction by sodium borohydride to produce a polyelectrolyte nanocomposite thin film containing metal nanoparticles.
27427666	0	31	theme	Composite	59:67	arg1	Nanoparticles					75:87	Chitosan/Polystyrene Sulfonate Multilayered Composite Metal Nanoparticles	15:87	Chitosan/Polystyrene Sulfonate Multilayered Composite Metal Nanoparticles	15:87	Preparation of Chitosan/Polystyrene Sulfonate Multilayered Composite Metal Nanoparticles and Its Application.
27427666	3	32	theme	absorption	632:641	arg1	spectroscopy					643:654	UV-visible absorption spectroscopy	621:654	UV-visible absorption spectroscopy (UV-vis)	621:663	Assembly, surface morphology and electrochemical properties of the composite membrane were analyzed by UV-visible absorption spectroscopy (UV-vis), atomic force microscopy (AFM) and cyclic voltammetry (CV).
27427666	3	33	dep	spectroscopy	643:654	arg1	UV-vis					657:662	UV-vis	657:662	UV-vis	657:662	Assembly, surface morphology and electrochemical properties of the composite membrane were analyzed by UV-visible absorption spectroscopy (UV-vis), atomic force microscopy (AFM) and cyclic voltammetry (CV).
27427666	1	34	theme	metal	215:219	arg1	ions					221:224	metal ions	215:224	metal ions	215:224	Metal-Chitosan (CTS) composite was first synthesized through the metal composition of chitosan (CTS) and metal ions.
27427666	0	35	theme	Multilayered	46:57	arg1	Nanoparticles					75:87	Chitosan/Polystyrene Sulfonate Multilayered Composite Metal Nanoparticles	15:87	Chitosan/Polystyrene Sulfonate Multilayered Composite Metal Nanoparticles	15:87	Preparation of Chitosan/Polystyrene Sulfonate Multilayered Composite Metal Nanoparticles and Its Application.
27427666	6	36	from	applications	1305:1316	arg1	sensors					1337:1343	electrochemical sensors	1321:1343	electrochemical sensors	1321:1343	The CV results indicated that the metal nanocomposite film had excellent electrocatalytic activity to glucose and had a potential for applications in electrochemical sensors.
27427666	4	37	theme	multilayer	940:949	arg1	film					951:954	the multilayer film	936:954	the multilayer film	936:954	The UV-Vis results indicated that the absorbance of the multilayer film at the characteristic absorption peak increased as the membrane bilayers increased, in a good linear relationship, which demonstrated that the multilayer film was uniformly assembled on the base.
27427666	3	38	theme	atomic	666:671	arg1	microscopy					679:688	atomic force microscopy	666:688	atomic force microscopy (AFM)	666:694	Assembly, surface morphology and electrochemical properties of the composite membrane were analyzed by UV-visible absorption spectroscopy (UV-vis), atomic force microscopy (AFM) and cyclic voltammetry (CV).
27427666	3	38	theme	atomic	666:671	arg1	AFM					691:693	AFM	691:693	AFM	691:693	Assembly, surface morphology and electrochemical properties of the composite membrane were analyzed by UV-visible absorption spectroscopy (UV-vis), atomic force microscopy (AFM) and cyclic voltammetry (CV).
27427666	2	39	theme	formed	231:236	arg1	composite					238:246	The formed composite	227:246	The formed composite	227:246	The formed composite was alternately deposited on the base with sodium polystyrene sulfonate (PSS) through a layer-by-layer self-assembling technique, followed by an in situ reduction by sodium borohydride to produce a polyelectrolyte nanocomposite thin film containing metal nanoparticles.
27427666	1	40	theme	ions	221:224	arg1	composition					181:191	the metal composition	171:191	the metal composition of chitosan (CTS) and metal ions	171:224	Metal-Chitosan (CTS) composite was first synthesized through the metal composition of chitosan (CTS) and metal ions.
27427666	0	41	theme	Nanoparticles	75:87	arg1	Application					97:107	Its Application	93:107	Its Application	93:107	Preparation of Chitosan/Polystyrene Sulfonate Multilayered Composite Metal Nanoparticles and Its Application.
27427666	0	41	theme	Nanoparticles	75:87	arg1	Preparation					0:10	Preparation	0:10	Preparation of Chitosan/Polystyrene Sulfonate Multilayered Composite Metal Nanoparticles	0:87	Preparation of Chitosan/Polystyrene Sulfonate Multilayered Composite Metal Nanoparticles and Its Application.
27427666	6	42	theme	electrochemical	1321:1335	arg1	sensors					1337:1343	electrochemical sensors	1321:1343	electrochemical sensors	1321:1343	The CV results indicated that the metal nanocomposite film had excellent electrocatalytic activity to glucose and had a potential for applications in electrochemical sensors.
27427666	3	43	theme	force	673:677	arg1	microscopy					679:688	atomic force microscopy	666:688	atomic force microscopy (AFM)	666:694	Assembly, surface morphology and electrochemical properties of the composite membrane were analyzed by UV-visible absorption spectroscopy (UV-vis), atomic force microscopy (AFM) and cyclic voltammetry (CV).
27427666	3	43	theme	force	673:677	arg1	AFM					691:693	AFM	691:693	AFM	691:693	Assembly, surface morphology and electrochemical properties of the composite membrane were analyzed by UV-visible absorption spectroscopy (UV-vis), atomic force microscopy (AFM) and cyclic voltammetry (CV).
27427666	2	44	theme	layer-by-layer	336:349	arg1	technique					367:375	a layer-by-layer self-assembling technique	334:375	a layer-by-layer self-assembling technique	334:375	The formed composite was alternately deposited on the base with sodium polystyrene sulfonate (PSS) through a layer-by-layer self-assembling technique, followed by an in situ reduction by sodium borohydride to produce a polyelectrolyte nanocomposite thin film containing metal nanoparticles.
27427666	0	45	theme	Metal	69:73	arg1	Nanoparticles					75:87	Chitosan/Polystyrene Sulfonate Multilayered Composite Metal Nanoparticles	15:87	Chitosan/Polystyrene Sulfonate Multilayered Composite Metal Nanoparticles	15:87	Preparation of Chitosan/Polystyrene Sulfonate Multilayered Composite Metal Nanoparticles and Its Application.
27427666	5	46	theme	multilayer	1035:1044	arg1	composite					1056:1064	the multilayer thin-film composite	1031:1064	the multilayer thin-film composite	1031:1064	AFM images showed that the surface of the multilayer thin-film composite had some degree of roughness and metal nanoparticles of 10-20 nm in size were generated on the membrane.
27427666	3	47	theme	UV-visible	621:630	arg1	spectroscopy					643:654	UV-visible absorption spectroscopy	621:654	UV-visible absorption spectroscopy (UV-vis)	621:663	Assembly, surface morphology and electrochemical properties of the composite membrane were analyzed by UV-visible absorption spectroscopy (UV-vis), atomic force microscopy (AFM) and cyclic voltammetry (CV).
27427666	3	48	theme	surface	528:534	arg1	morphology					536:545	surface morphology	528:545	surface morphology	528:545	Assembly, surface morphology and electrochemical properties of the composite membrane were analyzed by UV-visible absorption spectroscopy (UV-vis), atomic force microscopy (AFM) and cyclic voltammetry (CV).
27427666	5	49	theme	roughness	1085:1093	arg1	degree					1075:1080	some degree	1070:1080	some degree of roughness	1070:1093	AFM images showed that the surface of the multilayer thin-film composite had some degree of roughness and metal nanoparticles of 10-20 nm in size were generated on the membrane.
27427666	6	50	theme	nanocomposite	1211:1223	arg1	film					1225:1228	the metal nanocomposite film	1201:1228	the metal nanocomposite film	1201:1228	The CV results indicated that the metal nanocomposite film had excellent electrocatalytic activity to glucose and had a potential for applications in electrochemical sensors.
27427666	4	51	theme	characteristic	804:817	arg1	peak					830:833	the characteristic absorption peak	800:833	the characteristic absorption peak increased as the membrane bilayers	800:868	The UV-Vis results indicated that the absorbance of the multilayer film at the characteristic absorption peak increased as the membrane bilayers increased, in a good linear relationship, which demonstrated that the multilayer film was uniformly assembled on the base.
27427666	3	52	theme	cyclic	700:705	arg1	CV					720:721	CV	720:721	CV	720:721	Assembly, surface morphology and electrochemical properties of the composite membrane were analyzed by UV-visible absorption spectroscopy (UV-vis), atomic force microscopy (AFM) and cyclic voltammetry (CV).
27427666	3	52	theme	cyclic	700:705	arg1	voltammetry					707:717	cyclic voltammetry	700:717	cyclic voltammetry (CV)	700:722	Assembly, surface morphology and electrochemical properties of the composite membrane were analyzed by UV-visible absorption spectroscopy (UV-vis), atomic force microscopy (AFM) and cyclic voltammetry (CV).
27427666	6	53	theme	metal	1205:1209	arg1	film					1225:1228	the metal nanocomposite film	1201:1228	the metal nanocomposite film	1201:1228	The CV results indicated that the metal nanocomposite film had excellent electrocatalytic activity to glucose and had a potential for applications in electrochemical sensors.
27427666	4	54	from	peak	830:833	arg1	absorbance					763:772	the absorbance	759:772	the absorbance of the multilayer film at the characteristic absorption peak increased as the membrane bilayers	759:868	The UV-Vis results indicated that the absorbance of the multilayer film at the characteristic absorption peak increased as the membrane bilayers increased, in a good linear relationship, which demonstrated that the multilayer film was uniformly assembled on the base.
27427666	3	55	theme	composite	585:593	arg1	membrane					595:602	the composite membrane	581:602	the composite membrane	581:602	Assembly, surface morphology and electrochemical properties of the composite membrane were analyzed by UV-visible absorption spectroscopy (UV-vis), atomic force microscopy (AFM) and cyclic voltammetry (CV).
27427666	2	56	with	base	281:284	arg1	sulfonate					310:318	sodium polystyrene sulfonate	291:318	sodium polystyrene sulfonate (PSS)	291:324	The formed composite was alternately deposited on the base with sodium polystyrene sulfonate (PSS) through a layer-by-layer self-assembling technique, followed by an in situ reduction by sodium borohydride to produce a polyelectrolyte nanocomposite thin film containing metal nanoparticles.
27427666	2	56	with	base	281:284	arg1	PSS					321:323	PSS	321:323	PSS	321:323	The formed composite was alternately deposited on the base with sodium polystyrene sulfonate (PSS) through a layer-by-layer self-assembling technique, followed by an in situ reduction by sodium borohydride to produce a polyelectrolyte nanocomposite thin film containing metal nanoparticles.
27427666	5	57	theme	AFM	993:995	arg1	images					997:1002	AFM images	993:1002	AFM images	993:1002	AFM images showed that the surface of the multilayer thin-film composite had some degree of roughness and metal nanoparticles of 10-20 nm in size were generated on the membrane.
27427666	4	58	theme	UV-Vis	729:734	arg1	results					736:742	The UV-Vis results	725:742	The UV-Vis results	725:742	The UV-Vis results indicated that the absorbance of the multilayer film at the characteristic absorption peak increased as the membrane bilayers increased, in a good linear relationship, which demonstrated that the multilayer film was uniformly assembled on the base.
27427666	5	59	contain	had	1066:1068	arg2	degree					1075:1080	some degree	1070:1080	some degree of roughness	1070:1093	AFM images showed that the surface of the multilayer thin-film composite had some degree of roughness and metal nanoparticles of 10-20 nm in size were generated on the membrane.
27427666	5	59	contain	had	1066:1068	arg1	surface					1020:1026	the surface	1016:1026	the surface of the multilayer thin-film composite	1016:1064	AFM images showed that the surface of the multilayer thin-film composite had some degree of roughness and metal nanoparticles of 10-20 nm in size were generated on the membrane.
27427666	4	60	theme	absorption	819:828	arg1	peak					830:833	the characteristic absorption peak	800:833	the characteristic absorption peak increased as the membrane bilayers	800:868	The UV-Vis results indicated that the absorbance of the multilayer film at the characteristic absorption peak increased as the membrane bilayers increased, in a good linear relationship, which demonstrated that the multilayer film was uniformly assembled on the base.
27427666	2	61	theme	in	393:394	arg1	reduction					401:409	an in situ reduction	390:409	an in situ reduction by sodium borohydride to produce a polyelectrolyte nanocomposite thin film containing metal nanoparticles	390:515	The formed composite was alternately deposited on the base with sodium polystyrene sulfonate (PSS) through a layer-by-layer self-assembling technique, followed by an in situ reduction by sodium borohydride to produce a polyelectrolyte nanocomposite thin film containing metal nanoparticles.
27427666	2	62	theme	thin	476:479	arg1	film					481:484	a polyelectrolyte nanocomposite thin film	444:484	a polyelectrolyte nanocomposite thin film containing metal nanoparticles	444:515	The formed composite was alternately deposited on the base with sodium polystyrene sulfonate (PSS) through a layer-by-layer self-assembling technique, followed by an in situ reduction by sodium borohydride to produce a polyelectrolyte nanocomposite thin film containing metal nanoparticles.
27427666	5	63	theme	nm	1128:1129	arg1	nanoparticles					1105:1117	metal nanoparticles	1099:1117	metal nanoparticles of 10-20 nm in size	1099:1137	AFM images showed that the surface of the multilayer thin-film composite had some degree of roughness and metal nanoparticles of 10-20 nm in size were generated on the membrane.
27427666	2	64	dep	in	393:394	arg1	situ					396:399	situ	396:399	situ	396:399	The formed composite was alternately deposited on the base with sodium polystyrene sulfonate (PSS) through a layer-by-layer self-assembling technique, followed by an in situ reduction by sodium borohydride to produce a polyelectrolyte nanocomposite thin film containing metal nanoparticles.
27522435	12	0	from	similarity	1926:1935	arg1	porcine					2009:2015	porcine	2009:2015	porcine	2009:2015	The similarity of both intestinal physiopathology and milk OS composition in human and porcine species suggests similar effects on gastrointestinal development of early nutrition, reinforcing the use of the pig intestinal model to simulate human intestinal models in the clinical setting.
27522435	12	0	from	similarity	1926:1935	arg1	human					1999:2003	human	1999:2003	human	1999:2003	The similarity of both intestinal physiopathology and milk OS composition in human and porcine species suggests similar effects on gastrointestinal development of early nutrition, reinforcing the use of the pig intestinal model to simulate human intestinal models in the clinical setting.
27522435	6	1	theme	human	1061:1065	arg1	milk					1067:1070	human milk	1061:1070	human milk	1061:1070	Porcine milk had lower OS diversity (number of unique structures) than human milk, and appeared closer to bovine and caprine milk.
27522435	3	2	from	farrowing	496:504	arg1	sows					474:477	3 healthy sows	464:477	3 healthy sows at prefarrowing, farrowing, and d 7 and 14 postpartum	464:531	The evolution of PMO was investigated in the milk from 3 healthy sows at prefarrowing, farrowing, and d 7 and 14 postpartum by Nano-LC Chip Quadrupole-Time-of-Flight mass spectrometer (Agilent Technologies, Santa Clara, CA).
27522435	2	3	theme	striking	348:355	arg1	similarity					357:366	the striking similarity	344:366	the striking similarity in intestinal physiopathology to humans	344:406	Pigs are considered ideal intestinal models to simulate humans because of the striking similarity in intestinal physiopathology to humans.
27522435	3	4	theme	mass	575:578	arg1	spectrometer					580:591	Nano-LC Chip Quadrupole-Time-of-Flight mass spectrometer	536:591	Nano-LC Chip Quadrupole-Time-of-Flight mass spectrometer (Agilent Technologies, Santa Clara, CA)	536:631	The evolution of PMO was investigated in the milk from 3 healthy sows at prefarrowing, farrowing, and d 7 and 14 postpartum by Nano-LC Chip Quadrupole-Time-of-Flight mass spectrometer (Agilent Technologies, Santa Clara, CA).
27522435	12	5	theme	composition	1984:1994	arg1	similarity					1926:1935	The similarity	1922:1935	The similarity of both intestinal physiopathology and milk OS composition in human and porcine species	1922:2023	The similarity of both intestinal physiopathology and milk OS composition in human and porcine species suggests similar effects on gastrointestinal development of early nutrition, reinforcing the use of the pig intestinal model to simulate human intestinal models in the clinical setting.
27522435	4	6	theme	sequenced	645:653	arg1	libraries					666:674	Previously sequenced metagenome libraries	634:674	Previously sequenced metagenome libraries	634:674	Previously sequenced metagenome libraries were reanalyzed to examine changes with specific gut bacterial populations.
27522435	3	7	from	sows	474:477	arg1	milk					454:457	the milk	450:457	the milk from 3 healthy sows at prefarrowing, farrowing, and d 7 and 14 postpartum	450:531	The evolution of PMO was investigated in the milk from 3 healthy sows at prefarrowing, farrowing, and d 7 and 14 postpartum by Nano-LC Chip Quadrupole-Time-of-Flight mass spectrometer (Agilent Technologies, Santa Clara, CA).
27522435	11	8	theme	distal	1899:1904	arg1	microbiome					1910:1919	the distal gut microbiome	1895:1919	the distal gut microbiome	1895:1919	Metagenomic sequencing revealed that fucose-consuming bacterial taxa in the gut microbiota of piglets were qualitatively but not quantitatively different between nursing and weaning stages, suggesting that both the composition and structure of dietary glycans may play a critical role in shaping the distal gut microbiome.
27522435	3	9	dep	spectrometer	580:591	arg1	CA					629:630	CA	629:630	CA	629:630	The evolution of PMO was investigated in the milk from 3 healthy sows at prefarrowing, farrowing, and d 7 and 14 postpartum by Nano-LC Chip Quadrupole-Time-of-Flight mass spectrometer (Agilent Technologies, Santa Clara, CA).
27522435	3	9	dep	spectrometer	580:591	arg1	Technologies					602:613	Agilent Technologies	594:613	Agilent Technologies	594:613	The evolution of PMO was investigated in the milk from 3 healthy sows at prefarrowing, farrowing, and d 7 and 14 postpartum by Nano-LC Chip Quadrupole-Time-of-Flight mass spectrometer (Agilent Technologies, Santa Clara, CA).
27522435	3	9	dep	spectrometer	580:591	arg1	Clara					622:626	Clara	622:626	Clara	622:626	The evolution of PMO was investigated in the milk from 3 healthy sows at prefarrowing, farrowing, and d 7 and 14 postpartum by Nano-LC Chip Quadrupole-Time-of-Flight mass spectrometer (Agilent Technologies, Santa Clara, CA).
27522435	10	10	theme	abundance	1496:1504	arg1	terms					1487:1491	terms	1487:1491	terms of abundance	1487:1504	Taken together, these results suggest that, in terms of abundance, PMO are closer to human milk than other domestic species, such as bovine and caprine milks.
27522435	11	11	theme	piglets	1693:1699	arg1	microbiota					1679:1688	the gut microbiota	1671:1688	the gut microbiota of piglets	1671:1699	Metagenomic sequencing revealed that fucose-consuming bacterial taxa in the gut microbiota of piglets were qualitatively but not quantitatively different between nursing and weaning stages, suggesting that both the composition and structure of dietary glycans may play a critical role in shaping the distal gut microbiome.
27522435	3	12	from	prefarrowing	482:493	arg1	sows					474:477	3 healthy sows	464:477	3 healthy sows at prefarrowing, farrowing, and d 7 and 14 postpartum	464:531	The evolution of PMO was investigated in the milk from 3 healthy sows at prefarrowing, farrowing, and d 7 and 14 postpartum by Nano-LC Chip Quadrupole-Time-of-Flight mass spectrometer (Agilent Technologies, Santa Clara, CA).
27522435	3	13	theme	Quadrupole-Time-of-Flight	549:573	arg1	spectrometer					580:591	Nano-LC Chip Quadrupole-Time-of-Flight mass spectrometer	536:591	Nano-LC Chip Quadrupole-Time-of-Flight mass spectrometer (Agilent Technologies, Santa Clara, CA)	536:631	The evolution of PMO was investigated in the milk from 3 healthy sows at prefarrowing, farrowing, and d 7 and 14 postpartum by Nano-LC Chip Quadrupole-Time-of-Flight mass spectrometer (Agilent Technologies, Santa Clara, CA).
27522435	11	14	from	taxa	1663:1666	arg1	microbiota					1679:1688	the gut microbiota	1671:1688	the gut microbiota of piglets	1671:1699	Metagenomic sequencing revealed that fucose-consuming bacterial taxa in the gut microbiota of piglets were qualitatively but not quantitatively different between nursing and weaning stages, suggesting that both the composition and structure of dietary glycans may play a critical role in shaping the distal gut microbiome.
27522435	7	15	with	agreement	1124:1132	arg1	studies					1148:1154	previous studies	1139:1154	previous studies	1139:1154	In agreement with previous studies, only 3 fucosylated OS were identified.
27522435	3	16	theme	Nano-LC	536:542	arg1	spectrometer					580:591	Nano-LC Chip Quadrupole-Time-of-Flight mass spectrometer	536:591	Nano-LC Chip Quadrupole-Time-of-Flight mass spectrometer (Agilent Technologies, Santa Clara, CA)	536:631	The evolution of PMO was investigated in the milk from 3 healthy sows at prefarrowing, farrowing, and d 7 and 14 postpartum by Nano-LC Chip Quadrupole-Time-of-Flight mass spectrometer (Agilent Technologies, Santa Clara, CA).
27522435	8	17	theme	OS	1238:1239	arg1	abundance					1241:1249	total OS abundance	1232:1249	total OS abundance	1232:1249	Surprisingly, their contribution to total OS abundance was greater than in bovine milk (9 vs. 1%).
27522435	8	18	theme	total	1232:1236	arg1	abundance					1241:1249	total OS abundance	1232:1249	total OS abundance	1232:1249	Surprisingly, their contribution to total OS abundance was greater than in bovine milk (9 vs. 1%).
27522435	3	19	theme	Agilent	594:600	arg1	CA					629:630	CA	629:630	CA	629:630	The evolution of PMO was investigated in the milk from 3 healthy sows at prefarrowing, farrowing, and d 7 and 14 postpartum by Nano-LC Chip Quadrupole-Time-of-Flight mass spectrometer (Agilent Technologies, Santa Clara, CA).
27522435	3	19	theme	Agilent	594:600	arg1	Technologies					602:613	Agilent Technologies	594:613	Agilent Technologies	594:613	The evolution of PMO was investigated in the milk from 3 healthy sows at prefarrowing, farrowing, and d 7 and 14 postpartum by Nano-LC Chip Quadrupole-Time-of-Flight mass spectrometer (Agilent Technologies, Santa Clara, CA).
27522435	3	19	theme	Agilent	594:600	arg1	Clara					622:626	Clara	622:626	Clara	622:626	The evolution of PMO was investigated in the milk from 3 healthy sows at prefarrowing, farrowing, and d 7 and 14 postpartum by Nano-LC Chip Quadrupole-Time-of-Flight mass spectrometer (Agilent Technologies, Santa Clara, CA).
27522435	12	20	theme	OS	1981:1982	arg1	composition					1984:1994	milk OS composition	1976:1994	milk OS composition	1976:1994	The similarity of both intestinal physiopathology and milk OS composition in human and porcine species suggests similar effects on gastrointestinal development of early nutrition, reinforcing the use of the pig intestinal model to simulate human intestinal models in the clinical setting.
27522435	9	21	theme	OS	1405:1406	arg1	content					1408:1414	OS content	1405:1414	OS content	1405:1414	Indeed, fucosylated PMO increased during lactation, mirroring a similar trend observed for neutral and type I OS content during early lactation.
27522435	4	22	theme	gut	725:727	arg1	populations					739:749	specific gut bacterial populations	716:749	specific gut bacterial populations	716:749	Previously sequenced metagenome libraries were reanalyzed to examine changes with specific gut bacterial populations.
27522435	7	23	gly	fucosylated	1164:1174	arg1	OS					1176:1177	only 3 fucosylated OS	1157:1177	only 3 fucosylated OS	1157:1177	In agreement with previous studies, only 3 fucosylated OS were identified.
27522435	11	24	theme	fucose-consuming	1636:1651	arg1	taxa					1663:1666	fucose-consuming bacterial taxa	1636:1666	fucose-consuming bacterial taxa in the gut microbiota of piglets	1636:1699	Metagenomic sequencing revealed that fucose-consuming bacterial taxa in the gut microbiota of piglets were qualitatively but not quantitatively different between nursing and weaning stages, suggesting that both the composition and structure of dietary glycans may play a critical role in shaping the distal gut microbiome.
27522435	11	25	theme	gut	1906:1908	arg1	microbiome					1910:1919	the distal gut microbiome	1895:1919	the distal gut microbiome	1895:1919	Metagenomic sequencing revealed that fucose-consuming bacterial taxa in the gut microbiota of piglets were qualitatively but not quantitatively different between nursing and weaning stages, suggesting that both the composition and structure of dietary glycans may play a critical role in shaping the distal gut microbiome.
27522435	5	26	theme	abundant	934:941	arg1	species					943:949	the most abundant species	925:949	the most abundant species	925:949	Over 30 oligosaccharides (OS) were identified in the milk, with 3'-sialyllactose, lacto-N-tetraose, α1-3,β1-4-d-galactotriose, 2'-fucosyllactose, and 6'-sialyllactose being the most abundant species (accounting for ~70% of the total OS).
27522435	12	27	theme	intestinal	1945:1954	arg1	physiopathology					1956:1970	intestinal physiopathology	1945:1970	intestinal physiopathology	1945:1970	The similarity of both intestinal physiopathology and milk OS composition in human and porcine species suggests similar effects on gastrointestinal development of early nutrition, reinforcing the use of the pig intestinal model to simulate human intestinal models in the clinical setting.
27522435	3	28	theme	14	519:520	arg1	postpartum					522:531	d 7 and 14 postpartum	511:531	d 7 and 14 postpartum	511:531	The evolution of PMO was investigated in the milk from 3 healthy sows at prefarrowing, farrowing, and d 7 and 14 postpartum by Nano-LC Chip Quadrupole-Time-of-Flight mass spectrometer (Agilent Technologies, Santa Clara, CA).
27522435	1	29	theme	oligosaccharides	149:164	arg1	composition					121:131	The composition	117:131	The composition of porcine milk oligosaccharides (PMO)	117:170	The composition of porcine milk oligosaccharides (PMO) was analyzed during early lactation and their relation to piglet gut microbiome was investigated.
27522435	6	30	theme	Porcine	990:996	arg1	milk					998:1001	Porcine milk	990:1001	Porcine milk	990:1001	Porcine milk had lower OS diversity (number of unique structures) than human milk, and appeared closer to bovine and caprine milk.
27522435	7	31	theme	fucosylated	1164:1174	arg1	OS					1176:1177	only 3 fucosylated OS	1157:1177	only 3 fucosylated OS	1157:1177	In agreement with previous studies, only 3 fucosylated OS were identified.
27522435	6	32	dep	diversity	1016:1024	arg1	number					1027:1032	number	1027:1032	number of unique structures	1027:1053	Porcine milk had lower OS diversity (number of unique structures) than human milk, and appeared closer to bovine and caprine milk.
27522435	3	33	from	postpartum	522:531	arg1	sows					474:477	3 healthy sows	464:477	3 healthy sows at prefarrowing, farrowing, and d 7 and 14 postpartum	464:531	The evolution of PMO was investigated in the milk from 3 healthy sows at prefarrowing, farrowing, and d 7 and 14 postpartum by Nano-LC Chip Quadrupole-Time-of-Flight mass spectrometer (Agilent Technologies, Santa Clara, CA).
27522435	1	34	theme	porcine	136:142	arg1	oligosaccharides					149:164	porcine milk oligosaccharides	136:164	porcine milk oligosaccharides (PMO)	136:170	The composition of porcine milk oligosaccharides (PMO) was analyzed during early lactation and their relation to piglet gut microbiome was investigated.
27522435	1	34	theme	porcine	136:142	arg1	PMO					167:169	PMO	167:169	PMO	167:169	The composition of porcine milk oligosaccharides (PMO) was analyzed during early lactation and their relation to piglet gut microbiome was investigated.
27522435	12	35	theme	human	2162:2166	arg1	models					2179:2184	human intestinal models	2162:2184	human intestinal models	2162:2184	The similarity of both intestinal physiopathology and milk OS composition in human and porcine species suggests similar effects on gastrointestinal development of early nutrition, reinforcing the use of the pig intestinal model to simulate human intestinal models in the clinical setting.
27522435	11	36	theme	dietary	1843:1849	arg1	glycans					1851:1857	dietary glycans	1843:1857	dietary glycans	1843:1857	Metagenomic sequencing revealed that fucose-consuming bacterial taxa in the gut microbiota of piglets were qualitatively but not quantitatively different between nursing and weaning stages, suggesting that both the composition and structure of dietary glycans may play a critical role in shaping the distal gut microbiome.
27522435	6	37	contain	had	1003:1005	arg1	milk					998:1001	Porcine milk	990:1001	Porcine milk	990:1001	Porcine milk had lower OS diversity (number of unique structures) than human milk, and appeared closer to bovine and caprine milk.
27522435	6	37	contain	had	1003:1005	arg2	diversity					1016:1024	lower OS diversity	1007:1024	lower OS diversity (number of unique structures)	1007:1054	Porcine milk had lower OS diversity (number of unique structures) than human milk, and appeared closer to bovine and caprine milk.
27522435	10	38	theme	other	1541:1545	arg1	species					1556:1562	other domestic species	1541:1562	other domestic species	1541:1562	Taken together, these results suggest that, in terms of abundance, PMO are closer to human milk than other domestic species, such as bovine and caprine milks.
27522435	10	38	theme	other	1541:1545	arg1	milks					1592:1596	bovine and caprine milks	1573:1596	bovine and caprine milks	1573:1596	Taken together, these results suggest that, in terms of abundance, PMO are closer to human milk than other domestic species, such as bovine and caprine milks.
27522435	6	39	theme	structures	1044:1053	arg1	number					1027:1032	number	1027:1032	number of unique structures	1027:1053	Porcine milk had lower OS diversity (number of unique structures) than human milk, and appeared closer to bovine and caprine milk.
27522435	12	40	theme	pig	2129:2131	arg1	model					2144:2148	the pig intestinal model	2125:2148	the pig intestinal model	2125:2148	The similarity of both intestinal physiopathology and milk OS composition in human and porcine species suggests similar effects on gastrointestinal development of early nutrition, reinforcing the use of the pig intestinal model to simulate human intestinal models in the clinical setting.
27522435	0	41	theme	porcine	20:26	arg1	milk					28:31	porcine milk	20:31	porcine milk	20:31	Characterization of porcine milk oligosaccharides during early lactation and their relation to the fecal microbiome.
27522435	9	42	theme	early	1423:1427	arg1	lactation					1429:1437	early lactation	1423:1437	early lactation	1423:1437	Indeed, fucosylated PMO increased during lactation, mirroring a similar trend observed for neutral and type I OS content during early lactation.
27522435	9	43	gly	fucosylated	1303:1313	arg1	PMO					1315:1317	fucosylated PMO	1303:1317	fucosylated PMO	1303:1317	Indeed, fucosylated PMO increased during lactation, mirroring a similar trend observed for neutral and type I OS content during early lactation.
27522435	12	44	theme	similar	2034:2040	arg1	effects					2042:2048	similar effects	2034:2048	similar effects on gastrointestinal development of early nutrition	2034:2099	The similarity of both intestinal physiopathology and milk OS composition in human and porcine species suggests similar effects on gastrointestinal development of early nutrition, reinforcing the use of the pig intestinal model to simulate human intestinal models in the clinical setting.
27522435	6	45	theme	OS	1013:1014	arg1	diversity					1016:1024	lower OS diversity	1007:1024	lower OS diversity (number of unique structures)	1007:1054	Porcine milk had lower OS diversity (number of unique structures) than human milk, and appeared closer to bovine and caprine milk.
27522435	11	46	theme	nursing	1761:1767	arg1	stages					1781:1786	nursing and weaning stages	1761:1786	nursing and weaning stages	1761:1786	Metagenomic sequencing revealed that fucose-consuming bacterial taxa in the gut microbiota of piglets were qualitatively but not quantitatively different between nursing and weaning stages, suggesting that both the composition and structure of dietary glycans may play a critical role in shaping the distal gut microbiome.
27522435	0	47	theme	early	57:61	arg1	lactation					63:71	early lactation	57:71	early lactation	57:71	Characterization of porcine milk oligosaccharides during early lactation and their relation to the fecal microbiome.
27522435	11	48	dep	composition	1814:1824	arg1	the					1810:1812	the	1810:1812	the	1810:1812	Metagenomic sequencing revealed that fucose-consuming bacterial taxa in the gut microbiota of piglets were qualitatively but not quantitatively different between nursing and weaning stages, suggesting that both the composition and structure of dietary glycans may play a critical role in shaping the distal gut microbiome.
27522435	10	49	theme	bovine	1573:1578	arg1	milks					1592:1596	bovine and caprine milks	1573:1596	bovine and caprine milks	1573:1596	Taken together, these results suggest that, in terms of abundance, PMO are closer to human milk than other domestic species, such as bovine and caprine milks.
27522435	5	50	theme	OS	985:986	arg1	%					970:970	~70%	967:970	~70% of the total OS	967:986	Over 30 oligosaccharides (OS) were identified in the milk, with 3'-sialyllactose, lacto-N-tetraose, α1-3,β1-4-d-galactotriose, 2'-fucosyllactose, and 6'-sialyllactose being the most abundant species (accounting for ~70% of the total OS).
27522435	5	50	theme	OS	985:986	arg1	OS					985:986	the total OS	975:986	the total OS	975:986	Over 30 oligosaccharides (OS) were identified in the milk, with 3'-sialyllactose, lacto-N-tetraose, α1-3,β1-4-d-galactotriose, 2'-fucosyllactose, and 6'-sialyllactose being the most abundant species (accounting for ~70% of the total OS).
27522435	12	51	theme	early	2085:2089	arg1	nutrition					2091:2099	early nutrition	2085:2099	early nutrition	2085:2099	The similarity of both intestinal physiopathology and milk OS composition in human and porcine species suggests similar effects on gastrointestinal development of early nutrition, reinforcing the use of the pig intestinal model to simulate human intestinal models in the clinical setting.
27522435	2	52	theme	intestinal	371:380	arg1	physiopathology					382:396	intestinal physiopathology	371:396	intestinal physiopathology to humans	371:406	Pigs are considered ideal intestinal models to simulate humans because of the striking similarity in intestinal physiopathology to humans.
27522435	9	53	theme	similar	1359:1365	arg1	trend					1367:1371	a similar trend	1357:1371	a similar trend observed for neutral and type I OS content during early lactation	1357:1437	Indeed, fucosylated PMO increased during lactation, mirroring a similar trend observed for neutral and type I OS content during early lactation.
27522435	1	54	theme	piglet	230:235	arg1	microbiome					241:250	piglet gut microbiome	230:250	piglet gut microbiome	230:250	The composition of porcine milk oligosaccharides (PMO) was analyzed during early lactation and their relation to piglet gut microbiome was investigated.
27522435	11	55	theme	weaning	1773:1779	arg1	stages					1781:1786	nursing and weaning stages	1761:1786	nursing and weaning stages	1761:1786	Metagenomic sequencing revealed that fucose-consuming bacterial taxa in the gut microbiota of piglets were qualitatively but not quantitatively different between nursing and weaning stages, suggesting that both the composition and structure of dietary glycans may play a critical role in shaping the distal gut microbiome.
27522435	4	56	with	changes	703:709	arg1	populations					739:749	specific gut bacterial populations	716:749	specific gut bacterial populations	716:749	Previously sequenced metagenome libraries were reanalyzed to examine changes with specific gut bacterial populations.
27522435	2	57	theme	ideal	290:294	arg1	models					307:312	ideal intestinal models	290:312	ideal intestinal models	290:312	Pigs are considered ideal intestinal models to simulate humans because of the striking similarity in intestinal physiopathology to humans.
27522435	10	58	theme	caprine	1584:1590	arg1	milks					1592:1596	bovine and caprine milks	1573:1596	bovine and caprine milks	1573:1596	Taken together, these results suggest that, in terms of abundance, PMO are closer to human milk than other domestic species, such as bovine and caprine milks.
27522435	2	59	from	similarity	357:366	arg1	physiopathology					382:396	intestinal physiopathology	371:396	intestinal physiopathology to humans	371:406	Pigs are considered ideal intestinal models to simulate humans because of the striking similarity in intestinal physiopathology to humans.
27522435	3	60	theme	Chip	544:547	arg1	spectrometer					580:591	Nano-LC Chip Quadrupole-Time-of-Flight mass spectrometer	536:591	Nano-LC Chip Quadrupole-Time-of-Flight mass spectrometer (Agilent Technologies, Santa Clara, CA)	536:631	The evolution of PMO was investigated in the milk from 3 healthy sows at prefarrowing, farrowing, and d 7 and 14 postpartum by Nano-LC Chip Quadrupole-Time-of-Flight mass spectrometer (Agilent Technologies, Santa Clara, CA).
27522435	6	61	theme	caprine	1107:1113	arg1	milk					1115:1118	caprine milk	1107:1118	caprine milk	1107:1118	Porcine milk had lower OS diversity (number of unique structures) than human milk, and appeared closer to bovine and caprine milk.
27522435	4	62	theme	metagenome	655:664	arg1	libraries					666:674	Previously sequenced metagenome libraries	634:674	Previously sequenced metagenome libraries	634:674	Previously sequenced metagenome libraries were reanalyzed to examine changes with specific gut bacterial populations.
27522435	12	63	dep	human	1999:2003	arg1	species					2017:2023	species	2017:2023	species	2017:2023	The similarity of both intestinal physiopathology and milk OS composition in human and porcine species suggests similar effects on gastrointestinal development of early nutrition, reinforcing the use of the pig intestinal model to simulate human intestinal models in the clinical setting.
27522435	1	64	theme	early	192:196	arg1	lactation					198:206	early lactation	192:206	early lactation	192:206	The composition of porcine milk oligosaccharides (PMO) was analyzed during early lactation and their relation to piglet gut microbiome was investigated.
27522435	12	65	theme	physiopathology	1956:1970	arg1	similarity					1926:1935	The similarity	1922:1935	The similarity of both intestinal physiopathology and milk OS composition in human and porcine species	1922:2023	The similarity of both intestinal physiopathology and milk OS composition in human and porcine species suggests similar effects on gastrointestinal development of early nutrition, reinforcing the use of the pig intestinal model to simulate human intestinal models in the clinical setting.
27522435	11	66	theme	glycans	1851:1857	arg1	structure					1830:1838	structure	1830:1838	structure	1830:1838	Metagenomic sequencing revealed that fucose-consuming bacterial taxa in the gut microbiota of piglets were qualitatively but not quantitatively different between nursing and weaning stages, suggesting that both the composition and structure of dietary glycans may play a critical role in shaping the distal gut microbiome.
27522435	11	66	theme	glycans	1851:1857	arg1	composition					1814:1824	composition	1814:1824	composition	1814:1824	Metagenomic sequencing revealed that fucose-consuming bacterial taxa in the gut microbiota of piglets were qualitatively but not quantitatively different between nursing and weaning stages, suggesting that both the composition and structure of dietary glycans may play a critical role in shaping the distal gut microbiome.
27522435	12	67	theme	milk	1976:1979	arg1	composition					1984:1994	milk OS composition	1976:1994	milk OS composition	1976:1994	The similarity of both intestinal physiopathology and milk OS composition in human and porcine species suggests similar effects on gastrointestinal development of early nutrition, reinforcing the use of the pig intestinal model to simulate human intestinal models in the clinical setting.
27522435	5	68	theme	total	979:983	arg1	OS					985:986	the total OS	975:986	the total OS	975:986	Over 30 oligosaccharides (OS) were identified in the milk, with 3'-sialyllactose, lacto-N-tetraose, α1-3,β1-4-d-galactotriose, 2'-fucosyllactose, and 6'-sialyllactose being the most abundant species (accounting for ~70% of the total OS).
27522435	6	69	theme	lower	1007:1011	arg1	diversity					1016:1024	lower OS diversity	1007:1024	lower OS diversity (number of unique structures)	1007:1054	Porcine milk had lower OS diversity (number of unique structures) than human milk, and appeared closer to bovine and caprine milk.
27522435	12	70	theme	model	2144:2148	arg1	use					2118:2120	the use	2114:2120	the use of the pig intestinal model to simulate human intestinal models in the clinical setting	2114:2208	The similarity of both intestinal physiopathology and milk OS composition in human and porcine species suggests similar effects on gastrointestinal development of early nutrition, reinforcing the use of the pig intestinal model to simulate human intestinal models in the clinical setting.
27522435	4	71	theme	specific	716:723	arg1	populations					739:749	specific gut bacterial populations	716:749	specific gut bacterial populations	716:749	Previously sequenced metagenome libraries were reanalyzed to examine changes with specific gut bacterial populations.
27522435	11	72	theme	bacterial	1653:1661	arg1	taxa					1663:1666	fucose-consuming bacterial taxa	1636:1666	fucose-consuming bacterial taxa in the gut microbiota of piglets	1636:1699	Metagenomic sequencing revealed that fucose-consuming bacterial taxa in the gut microbiota of piglets were qualitatively but not quantitatively different between nursing and weaning stages, suggesting that both the composition and structure of dietary glycans may play a critical role in shaping the distal gut microbiome.
27522435	11	73	theme	critical	1870:1877	arg1	role					1879:1882	a critical role	1868:1882	a critical role	1868:1882	Metagenomic sequencing revealed that fucose-consuming bacterial taxa in the gut microbiota of piglets were qualitatively but not quantitatively different between nursing and weaning stages, suggesting that both the composition and structure of dietary glycans may play a critical role in shaping the distal gut microbiome.
27522435	1	74	theme	milk	144:147	arg1	oligosaccharides					149:164	porcine milk oligosaccharides	136:164	porcine milk oligosaccharides (PMO)	136:170	The composition of porcine milk oligosaccharides (PMO) was analyzed during early lactation and their relation to piglet gut microbiome was investigated.
27522435	1	74	theme	milk	144:147	arg1	PMO					167:169	PMO	167:169	PMO	167:169	The composition of porcine milk oligosaccharides (PMO) was analyzed during early lactation and their relation to piglet gut microbiome was investigated.
27522435	12	75	theme	clinical	2193:2200	arg1	setting					2202:2208	the clinical setting	2189:2208	the clinical setting	2189:2208	The similarity of both intestinal physiopathology and milk OS composition in human and porcine species suggests similar effects on gastrointestinal development of early nutrition, reinforcing the use of the pig intestinal model to simulate human intestinal models in the clinical setting.
27522435	8	76	theme	bovine	1271:1276	arg1	milk					1278:1281	bovine milk	1271:1281	bovine milk	1271:1281	Surprisingly, their contribution to total OS abundance was greater than in bovine milk (9 vs. 1%).
27522435	11	77	theme	gut	1675:1677	arg1	microbiota					1679:1688	the gut microbiota	1671:1688	the gut microbiota of piglets	1671:1699	Metagenomic sequencing revealed that fucose-consuming bacterial taxa in the gut microbiota of piglets were qualitatively but not quantitatively different between nursing and weaning stages, suggesting that both the composition and structure of dietary glycans may play a critical role in shaping the distal gut microbiome.
27522435	0	78	theme	fecal	99:103	arg1	microbiome					105:114	the fecal microbiome	95:114	the fecal microbiome	95:114	Characterization of porcine milk oligosaccharides during early lactation and their relation to the fecal microbiome.
27522435	12	79	from	effects	2042:2048	arg1	development					2070:2080	gastrointestinal development	2053:2080	gastrointestinal development of early nutrition	2053:2099	The similarity of both intestinal physiopathology and milk OS composition in human and porcine species suggests similar effects on gastrointestinal development of early nutrition, reinforcing the use of the pig intestinal model to simulate human intestinal models in the clinical setting.
27522435	2	80	theme	intestinal	296:305	arg1	models					307:312	ideal intestinal models	290:312	ideal intestinal models	290:312	Pigs are considered ideal intestinal models to simulate humans because of the striking similarity in intestinal physiopathology to humans.
27522435	12	81	theme	intestinal	2168:2177	arg1	models					2179:2184	human intestinal models	2162:2184	human intestinal models	2162:2184	The similarity of both intestinal physiopathology and milk OS composition in human and porcine species suggests similar effects on gastrointestinal development of early nutrition, reinforcing the use of the pig intestinal model to simulate human intestinal models in the clinical setting.
27522435	12	82	theme	intestinal	2133:2142	arg1	model					2144:2148	the pig intestinal model	2125:2148	the pig intestinal model	2125:2148	The similarity of both intestinal physiopathology and milk OS composition in human and porcine species suggests similar effects on gastrointestinal development of early nutrition, reinforcing the use of the pig intestinal model to simulate human intestinal models in the clinical setting.
27522435	7	83	theme	previous	1139:1146	arg1	studies					1148:1154	previous studies	1139:1154	previous studies	1139:1154	In agreement with previous studies, only 3 fucosylated OS were identified.
27522435	11	84	dep	quantitatively	1728:1741	arg1	not					1724:1726	not	1724:1726	not	1724:1726	Metagenomic sequencing revealed that fucose-consuming bacterial taxa in the gut microbiota of piglets were qualitatively but not quantitatively different between nursing and weaning stages, suggesting that both the composition and structure of dietary glycans may play a critical role in shaping the distal gut microbiome.
27522435	6	85	theme	unique	1037:1042	arg1	structures					1044:1053	unique structures	1037:1053	unique structures	1037:1053	Porcine milk had lower OS diversity (number of unique structures) than human milk, and appeared closer to bovine and caprine milk.
27522435	4	86	theme	bacterial	729:737	arg1	populations					739:749	specific gut bacterial populations	716:749	specific gut bacterial populations	716:749	Previously sequenced metagenome libraries were reanalyzed to examine changes with specific gut bacterial populations.
27522435	10	87	theme	human	1525:1529	arg1	milk					1531:1534	human milk	1525:1534	human milk	1525:1534	Taken together, these results suggest that, in terms of abundance, PMO are closer to human milk than other domestic species, such as bovine and caprine milks.
27522435	0	88	theme	milk	28:31	arg1	Characterization					0:15	Characterization	0:15	Characterization of porcine milk	0:31	Characterization of porcine milk oligosaccharides during early lactation and their relation to the fecal microbiome.
27522435	3	89	theme	healthy	466:472	arg1	sows					474:477	3 healthy sows	464:477	3 healthy sows at prefarrowing, farrowing, and d 7 and 14 postpartum	464:531	The evolution of PMO was investigated in the milk from 3 healthy sows at prefarrowing, farrowing, and d 7 and 14 postpartum by Nano-LC Chip Quadrupole-Time-of-Flight mass spectrometer (Agilent Technologies, Santa Clara, CA).
27522435	3	90	theme	PMO	426:428	arg1	evolution					413:421	The evolution	409:421	The evolution of PMO	409:428	The evolution of PMO was investigated in the milk from 3 healthy sows at prefarrowing, farrowing, and d 7 and 14 postpartum by Nano-LC Chip Quadrupole-Time-of-Flight mass spectrometer (Agilent Technologies, Santa Clara, CA).
27522435	12	91	theme	nutrition	2091:2099	arg1	development					2070:2080	gastrointestinal development	2053:2080	gastrointestinal development of early nutrition	2053:2099	The similarity of both intestinal physiopathology and milk OS composition in human and porcine species suggests similar effects on gastrointestinal development of early nutrition, reinforcing the use of the pig intestinal model to simulate human intestinal models in the clinical setting.
27522435	10	92	theme	domestic	1547:1554	arg1	species					1556:1562	other domestic species	1541:1562	other domestic species	1541:1562	Taken together, these results suggest that, in terms of abundance, PMO are closer to human milk than other domestic species, such as bovine and caprine milks.
27522435	10	92	theme	domestic	1547:1554	arg1	milks					1592:1596	bovine and caprine milks	1573:1596	bovine and caprine milks	1573:1596	Taken together, these results suggest that, in terms of abundance, PMO are closer to human milk than other domestic species, such as bovine and caprine milks.
27522435	11	93	theme	Metagenomic	1599:1609	arg1	sequencing					1611:1620	Metagenomic sequencing	1599:1620	Metagenomic sequencing	1599:1620	Metagenomic sequencing revealed that fucose-consuming bacterial taxa in the gut microbiota of piglets were qualitatively but not quantitatively different between nursing and weaning stages, suggesting that both the composition and structure of dietary glycans may play a critical role in shaping the distal gut microbiome.
27522435	8	94	dep	greater	1255:1261	arg1	%					1291:1291	9 vs. 1%	1284:1291	9 vs. 1%	1284:1291	Surprisingly, their contribution to total OS abundance was greater than in bovine milk (9 vs. 1%).
27522435	12	95	theme	gastrointestinal	2053:2068	arg1	development					2070:2080	gastrointestinal development	2053:2080	gastrointestinal development of early nutrition	2053:2099	The similarity of both intestinal physiopathology and milk OS composition in human and porcine species suggests similar effects on gastrointestinal development of early nutrition, reinforcing the use of the pig intestinal model to simulate human intestinal models in the clinical setting.
27522435	9	96	theme	fucosylated	1303:1313	arg1	PMO					1315:1317	fucosylated PMO	1303:1317	fucosylated PMO	1303:1317	Indeed, fucosylated PMO increased during lactation, mirroring a similar trend observed for neutral and type I OS content during early lactation.
27522435	9	97	dep	neutral	1386:1392	arg1	content					1408:1414	OS content	1405:1414	OS content	1405:1414	Indeed, fucosylated PMO increased during lactation, mirroring a similar trend observed for neutral and type I OS content during early lactation.
27522435	3	98	theme	d	511:511	arg1	postpartum					522:531	d 7 and 14 postpartum	511:531	d 7 and 14 postpartum	511:531	The evolution of PMO was investigated in the milk from 3 healthy sows at prefarrowing, farrowing, and d 7 and 14 postpartum by Nano-LC Chip Quadrupole-Time-of-Flight mass spectrometer (Agilent Technologies, Santa Clara, CA).
27522435	1	99	theme	gut	237:239	arg1	microbiome					241:250	piglet gut microbiome	230:250	piglet gut microbiome	230:250	The composition of porcine milk oligosaccharides (PMO) was analyzed during early lactation and their relation to piglet gut microbiome was investigated.
26598647	6	0	theme	relative	1216:1223	arg1	compositions					1233:1244	the relative protein compositions	1212:1244	the relative protein compositions of isolated MVs and MV-releasing osteoblasts	1212:1289	Furthermore, we used comparative proteomic methods to study the relative protein compositions of isolated MVs and MV-releasing osteoblasts.
26598647	8	1	theme	matrix	1474:1479	arg1	activity					1489:1496	matrix vesicle activity	1474:1496	matrix vesicle activity	1474:1496	GAGs, especially sHA1, were found to severely impact vesicle-extracellular matrix interaction and matrix vesicle activity, leading to stronger extracellular matrix formation and mineralization.
26598647	0	2	theme	Sulfated	79:86	arg1	Glycosaminoglycans					105:122	Sulfated and Non-sulfated Glycosaminoglycans	79:122	Sulfated and Non-sulfated Glycosaminoglycans	79:122	Osteoblast-released Matrix Vesicles, Regulation of Activity and Composition by Sulfated and Non-sulfated Glycosaminoglycans.
26598647	5	3	theme	HA	1081:1082	arg1	effects					1064:1070	the molecular effects	1050:1070	the molecular effects of sHA1, HA, and CS1 on MV activity and on the distribution of marker proteins	1050:1149	Here, we studied the molecular effects of sHA1, HA, and CS1 on MV activity and on the distribution of marker proteins.
26598647	9	4	theme	important	1629:1637	arg1	regulation					1596:1605	the regulation	1592:1605	the regulation of MV activity	1592:1620	This study shows that the regulation of MV activity is one important mode of action of GAGs and provides information on underlying molecular mechanisms.
26598647	9	4	theme	important	1629:1637	arg1	mode					1639:1642	one important mode	1625:1642	one important mode of action of GAGs	1625:1660	This study shows that the regulation of MV activity is one important mode of action of GAGs and provides information on underlying molecular mechanisms.
26598647	2	5	theme	bone	365:368	arg1	environment					370:380	the native bone environment	354:380	the native bone environment	354:380	One promising therapeutic approach involves the coating of implants with modified glycosaminoglycans (GAGs) that mimic the native bone environment and actively facilitate skeletogenesis.
26598647	4	6	theme	osteoblast-released	925:943	arg1	MV					962:963	MV	962:963	MV	962:963	However, it remained open whether GAGs influence the osteoblasts alone or whether they also directly affect the formation, composition, activity, and distribution of osteoblast-released matrix vesicles (MV), which are supposed to be the active machinery for bone formation.
26598647	4	6	theme	osteoblast-released	925:943	arg1	vesicles					952:959	osteoblast-released matrix vesicles	925:959	osteoblast-released matrix vesicles (MV)	925:964	However, it remained open whether GAGs influence the osteoblasts alone or whether they also directly affect the formation, composition, activity, and distribution of osteoblast-released matrix vesicles (MV), which are supposed to be the active machinery for bone formation.
26598647	4	6	theme	osteoblast-released	925:943	arg1	machinery					1003:1011	the active machinery	992:1011	the active machinery for bone formation	992:1030	However, it remained open whether GAGs influence the osteoblasts alone or whether they also directly affect the formation, composition, activity, and distribution of osteoblast-released matrix vesicles (MV), which are supposed to be the active machinery for bone formation.
26598647	8	7	theme	vesicle-extracellular	1429:1449	arg1	interaction					1458:1468	vesicle-extracellular matrix interaction	1429:1468	vesicle-extracellular matrix interaction	1429:1468	GAGs, especially sHA1, were found to severely impact vesicle-extracellular matrix interaction and matrix vesicle activity, leading to stronger extracellular matrix formation and mineralization.
26598647	9	8	theme	MV	1610:1611	arg1	activity					1613:1620	MV activity	1610:1620	MV activity	1610:1620	This study shows that the regulation of MV activity is one important mode of action of GAGs and provides information on underlying molecular mechanisms.
26598647	2	9	theme	modified	308:315	arg1	GAGs					337:340	GAGs	337:340	GAGs	337:340	One promising therapeutic approach involves the coating of implants with modified glycosaminoglycans (GAGs) that mimic the native bone environment and actively facilitate skeletogenesis.
26598647	2	9	theme	modified	308:315	arg1	glycosaminoglycans					317:334	modified glycosaminoglycans	308:334	modified glycosaminoglycans (GAGs) that mimic the native bone environment and actively facilitate skeletogenesis	308:419	One promising therapeutic approach involves the coating of implants with modified glycosaminoglycans (GAGs) that mimic the native bone environment and actively facilitate skeletogenesis.
26598647	5	10	theme	marker	1135:1140	arg1	proteins					1142:1149	marker proteins	1135:1149	marker proteins	1135:1149	Here, we studied the molecular effects of sHA1, HA, and CS1 on MV activity and on the distribution of marker proteins.
26598647	0	11	theme	Non-sulfated	92:103	arg1	Glycosaminoglycans					105:122	Sulfated and Non-sulfated Glycosaminoglycans	79:122	Sulfated and Non-sulfated Glycosaminoglycans	79:122	Osteoblast-released Matrix Vesicles, Regulation of Activity and Composition by Sulfated and Non-sulfated Glycosaminoglycans.
26598647	9	12	theme	activity	1613:1620	arg1	regulation					1596:1605	the regulation	1592:1605	the regulation of MV activity	1592:1620	This study shows that the regulation of MV activity is one important mode of action of GAGs and provides information on underlying molecular mechanisms.
26598647	9	12	theme	activity	1613:1620	arg1	mode					1639:1642	one important mode	1625:1642	one important mode of action of GAGs	1625:1660	This study shows that the regulation of MV activity is one important mode of action of GAGs and provides information on underlying molecular mechanisms.
26598647	5	13	theme	proteins	1142:1149	arg1	distribution					1119:1130	the distribution	1115:1130	the distribution of marker proteins	1115:1149	Here, we studied the molecular effects of sHA1, HA, and CS1 on MV activity and on the distribution of marker proteins.
26598647	4	14	theme	vesicles	952:959	arg1	composition					882:892	composition	882:892	composition	882:892	However, it remained open whether GAGs influence the osteoblasts alone or whether they also directly affect the formation, composition, activity, and distribution of osteoblast-released matrix vesicles (MV), which are supposed to be the active machinery for bone formation.
26598647	4	14	theme	vesicles	952:959	arg1	formation					871:879	formation	871:879	formation	871:879	However, it remained open whether GAGs influence the osteoblasts alone or whether they also directly affect the formation, composition, activity, and distribution of osteoblast-released matrix vesicles (MV), which are supposed to be the active machinery for bone formation.
26598647	4	14	theme	vesicles	952:959	arg1	activity					895:902	activity	895:902	activity	895:902	However, it remained open whether GAGs influence the osteoblasts alone or whether they also directly affect the formation, composition, activity, and distribution of osteoblast-released matrix vesicles (MV), which are supposed to be the active machinery for bone formation.
26598647	4	14	theme	vesicles	952:959	arg1	distribution					909:920	distribution	909:920	distribution	909:920	However, it remained open whether GAGs influence the osteoblasts alone or whether they also directly affect the formation, composition, activity, and distribution of osteoblast-released matrix vesicles (MV), which are supposed to be the active machinery for bone formation.
26598647	1	15	theme	aging	129:133	arg1	population					135:144	Our aging population	125:144	Our aging population	125:144	Our aging population has to deal with the increasing threat of age-related diseases that impair bone healing.
26598647	2	16	theme	implants	294:301	arg1	coating					283:289	the coating	279:289	the coating of implants with modified glycosaminoglycans (GAGs) that mimic the native bone environment and actively facilitate skeletogenesis	279:419	One promising therapeutic approach involves the coating of implants with modified glycosaminoglycans (GAGs) that mimic the native bone environment and actively facilitate skeletogenesis.
26598647	4	17	theme	matrix	945:950	arg1	MV					962:963	MV	962:963	MV	962:963	However, it remained open whether GAGs influence the osteoblasts alone or whether they also directly affect the formation, composition, activity, and distribution of osteoblast-released matrix vesicles (MV), which are supposed to be the active machinery for bone formation.
26598647	4	17	theme	matrix	945:950	arg1	vesicles					952:959	osteoblast-released matrix vesicles	925:959	osteoblast-released matrix vesicles (MV)	925:964	However, it remained open whether GAGs influence the osteoblasts alone or whether they also directly affect the formation, composition, activity, and distribution of osteoblast-released matrix vesicles (MV), which are supposed to be the active machinery for bone formation.
26598647	4	17	theme	matrix	945:950	arg1	machinery					1003:1011	the active machinery	992:1011	the active machinery for bone formation	992:1030	However, it remained open whether GAGs influence the osteoblasts alone or whether they also directly affect the formation, composition, activity, and distribution of osteoblast-released matrix vesicles (MV), which are supposed to be the active machinery for bone formation.
26598647	8	18	theme	vesicle	1481:1487	arg1	activity					1489:1496	matrix vesicle activity	1474:1496	matrix vesicle activity	1474:1496	GAGs, especially sHA1, were found to severely impact vesicle-extracellular matrix interaction and matrix vesicle activity, leading to stronger extracellular matrix formation and mineralization.
26598647	6	19	theme	proteomic	1185:1193	arg1	methods					1195:1201	comparative proteomic methods	1173:1201	comparative proteomic methods	1173:1201	Furthermore, we used comparative proteomic methods to study the relative protein compositions of isolated MVs and MV-releasing osteoblasts.
26598647	7	20	theme	cellular	1357:1364	arg1	proteome					1366:1373	the cellular proteome	1353:1373	the cellular proteome	1353:1373	The MV proteome is much more strongly regulated by GAGs than the cellular proteome.
26598647	0	21	theme	Matrix	20:25	arg1	Vesicles					27:34	Osteoblast-released Matrix Vesicles	0:34	Osteoblast-released Matrix Vesicles	0:34	Osteoblast-released Matrix Vesicles, Regulation of Activity and Composition by Sulfated and Non-sulfated Glycosaminoglycans.
26598647	0	21	theme	Matrix	20:25	arg1	Regulation					37:46	Regulation	37:46	Regulation of Activity and Composition by Sulfated and Non-sulfated Glycosaminoglycans	37:122	Osteoblast-released Matrix Vesicles, Regulation of Activity and Composition by Sulfated and Non-sulfated Glycosaminoglycans.
26598647	4	22	dep	formation	871:879	arg1	the					867:869	the	867:869	the	867:869	However, it remained open whether GAGs influence the osteoblasts alone or whether they also directly affect the formation, composition, activity, and distribution of osteoblast-released matrix vesicles (MV), which are supposed to be the active machinery for bone formation.
26598647	6	23	theme	comparative	1173:1183	arg1	methods					1195:1201	comparative proteomic methods	1173:1201	comparative proteomic methods	1173:1201	Furthermore, we used comparative proteomic methods to study the relative protein compositions of isolated MVs and MV-releasing osteoblasts.
26598647	9	24	theme	action	1647:1652	arg1	regulation					1596:1605	the regulation	1592:1605	the regulation of MV activity	1592:1620	This study shows that the regulation of MV activity is one important mode of action of GAGs and provides information on underlying molecular mechanisms.
26598647	9	24	theme	action	1647:1652	arg1	mode					1639:1642	one important mode	1625:1642	one important mode of action of GAGs	1625:1660	This study shows that the regulation of MV activity is one important mode of action of GAGs and provides information on underlying molecular mechanisms.
26598647	5	25	theme	molecular	1054:1062	arg1	effects					1064:1070	the molecular effects	1050:1070	the molecular effects of sHA1, HA, and CS1 on MV activity and on the distribution of marker proteins	1050:1149	Here, we studied the molecular effects of sHA1, HA, and CS1 on MV activity and on the distribution of marker proteins.
26598647	1	26	theme	age-related	188:198	arg1	diseases					200:207	age-related diseases	188:207	age-related diseases that impair bone healing	188:232	Our aging population has to deal with the increasing threat of age-related diseases that impair bone healing.
26598647	0	27	theme	Osteoblast-released	0:18	arg1	Vesicles					27:34	Osteoblast-released Matrix Vesicles	0:34	Osteoblast-released Matrix Vesicles	0:34	Osteoblast-released Matrix Vesicles, Regulation of Activity and Composition by Sulfated and Non-sulfated Glycosaminoglycans.
26598647	0	27	theme	Osteoblast-released	0:18	arg1	Regulation					37:46	Regulation	37:46	Regulation of Activity and Composition by Sulfated and Non-sulfated Glycosaminoglycans	37:122	Osteoblast-released Matrix Vesicles, Regulation of Activity and Composition by Sulfated and Non-sulfated Glycosaminoglycans.
26598647	9	28	theme	GAGs	1657:1660	arg1	action					1647:1652	action	1647:1652	action of GAGs	1647:1660	This study shows that the regulation of MV activity is one important mode of action of GAGs and provides information on underlying molecular mechanisms.
26598647	9	29	theme	underlying	1690:1699	arg1	mechanisms					1711:1720	underlying molecular mechanisms	1690:1720	underlying molecular mechanisms	1690:1720	This study shows that the regulation of MV activity is one important mode of action of GAGs and provides information on underlying molecular mechanisms.
26598647	6	30	used	used	1168:1171	arg2	we					1165:1166	we	1165:1166	we	1165:1166	Furthermore, we used comparative proteomic methods to study the relative protein compositions of isolated MVs and MV-releasing osteoblasts.
26598647	1	31	theme	diseases	200:207	arg1	threat					178:183	the increasing threat	163:183	the increasing threat of age-related diseases that impair bone healing	163:232	Our aging population has to deal with the increasing threat of age-related diseases that impair bone healing.
26598647	6	32	theme	osteoblasts	1279:1289	arg1	compositions					1233:1244	the relative protein compositions	1212:1244	the relative protein compositions of isolated MVs and MV-releasing osteoblasts	1212:1289	Furthermore, we used comparative proteomic methods to study the relative protein compositions of isolated MVs and MV-releasing osteoblasts.
26598647	3	33	theme	low-sulfated	589:600	arg1	sulfate					618:624	the naturally low-sulfated GAG chondroitin sulfate	575:624	the naturally low-sulfated GAG chondroitin sulfate (CS1)	575:630	In previous studies, we reported that coatings containing GAGs, such as hyaluronic acid (HA) and its synthetically sulfated derivative (sHA1) as well as the naturally low-sulfated GAG chondroitin sulfate (CS1), reduce the activity of bone-resorbing osteoclasts, but they also induce functions of the bone-forming cells, the osteoblasts.
26598647	3	33	theme	low-sulfated	589:600	arg1	CS1					627:629	CS1	627:629	CS1	627:629	In previous studies, we reported that coatings containing GAGs, such as hyaluronic acid (HA) and its synthetically sulfated derivative (sHA1) as well as the naturally low-sulfated GAG chondroitin sulfate (CS1), reduce the activity of bone-resorbing osteoclasts, but they also induce functions of the bone-forming cells, the osteoblasts.
26598647	4	34	theme	active	996:1001	arg1	vesicles					952:959	osteoblast-released matrix vesicles	925:959	osteoblast-released matrix vesicles (MV)	925:964	However, it remained open whether GAGs influence the osteoblasts alone or whether they also directly affect the formation, composition, activity, and distribution of osteoblast-released matrix vesicles (MV), which are supposed to be the active machinery for bone formation.
26598647	4	34	theme	active	996:1001	arg1	machinery					1003:1011	the active machinery	992:1011	the active machinery for bone formation	992:1030	However, it remained open whether GAGs influence the osteoblasts alone or whether they also directly affect the formation, composition, activity, and distribution of osteoblast-released matrix vesicles (MV), which are supposed to be the active machinery for bone formation.
26598647	3	35	theme	bone-forming	722:733	arg1	cells					735:739	the bone-forming cells	718:739	the bone-forming cells	718:739	In previous studies, we reported that coatings containing GAGs, such as hyaluronic acid (HA) and its synthetically sulfated derivative (sHA1) as well as the naturally low-sulfated GAG chondroitin sulfate (CS1), reduce the activity of bone-resorbing osteoclasts, but they also induce functions of the bone-forming cells, the osteoblasts.
26598647	3	35	theme	bone-forming	722:733	arg1	osteoblasts					746:756	the osteoblasts	742:756	the osteoblasts	742:756	In previous studies, we reported that coatings containing GAGs, such as hyaluronic acid (HA) and its synthetically sulfated derivative (sHA1) as well as the naturally low-sulfated GAG chondroitin sulfate (CS1), reduce the activity of bone-resorbing osteoclasts, but they also induce functions of the bone-forming cells, the osteoblasts.
26598647	3	36	theme	sulfated	537:544	arg1	sHA1					558:561	sHA1	558:561	sHA1	558:561	In previous studies, we reported that coatings containing GAGs, such as hyaluronic acid (HA) and its synthetically sulfated derivative (sHA1) as well as the naturally low-sulfated GAG chondroitin sulfate (CS1), reduce the activity of bone-resorbing osteoclasts, but they also induce functions of the bone-forming cells, the osteoblasts.
26598647	3	36	theme	sulfated	537:544	arg1	derivative					546:555	its synthetically sulfated derivative	519:555	its synthetically sulfated derivative (sHA1)	519:562	In previous studies, we reported that coatings containing GAGs, such as hyaluronic acid (HA) and its synthetically sulfated derivative (sHA1) as well as the naturally low-sulfated GAG chondroitin sulfate (CS1), reduce the activity of bone-resorbing osteoclasts, but they also induce functions of the bone-forming cells, the osteoblasts.
26598647	1	37	theme	increasing	167:176	arg1	threat					178:183	the increasing threat	163:183	the increasing threat of age-related diseases that impair bone healing	163:232	Our aging population has to deal with the increasing threat of age-related diseases that impair bone healing.
26598647	3	38	theme	cells	735:739	arg1	functions					705:713	functions	705:713	functions of the bone-forming cells, the osteoblasts	705:756	In previous studies, we reported that coatings containing GAGs, such as hyaluronic acid (HA) and its synthetically sulfated derivative (sHA1) as well as the naturally low-sulfated GAG chondroitin sulfate (CS1), reduce the activity of bone-resorbing osteoclasts, but they also induce functions of the bone-forming cells, the osteoblasts.
26598647	2	39	theme	therapeutic	249:259	arg1	approach					261:268	One promising therapeutic approach	235:268	One promising therapeutic approach	235:268	One promising therapeutic approach involves the coating of implants with modified glycosaminoglycans (GAGs) that mimic the native bone environment and actively facilitate skeletogenesis.
26598647	1	40	theme	bone	221:224	arg1	healing					226:232	bone healing	221:232	bone healing	221:232	Our aging population has to deal with the increasing threat of age-related diseases that impair bone healing.
26598647	8	41	theme	extracellular	1519:1531	arg1	formation					1540:1548	stronger extracellular matrix formation	1510:1548	stronger extracellular matrix formation	1510:1548	GAGs, especially sHA1, were found to severely impact vesicle-extracellular matrix interaction and matrix vesicle activity, leading to stronger extracellular matrix formation and mineralization.
26598647	6	42	theme	MVs	1258:1260	arg1	compositions					1233:1244	the relative protein compositions	1212:1244	the relative protein compositions of isolated MVs and MV-releasing osteoblasts	1212:1289	Furthermore, we used comparative proteomic methods to study the relative protein compositions of isolated MVs and MV-releasing osteoblasts.
26598647	3	43	theme	GAG	602:604	arg1	sulfate					618:624	the naturally low-sulfated GAG chondroitin sulfate	575:624	the naturally low-sulfated GAG chondroitin sulfate (CS1)	575:630	In previous studies, we reported that coatings containing GAGs, such as hyaluronic acid (HA) and its synthetically sulfated derivative (sHA1) as well as the naturally low-sulfated GAG chondroitin sulfate (CS1), reduce the activity of bone-resorbing osteoclasts, but they also induce functions of the bone-forming cells, the osteoblasts.
26598647	3	43	theme	GAG	602:604	arg1	CS1					627:629	CS1	627:629	CS1	627:629	In previous studies, we reported that coatings containing GAGs, such as hyaluronic acid (HA) and its synthetically sulfated derivative (sHA1) as well as the naturally low-sulfated GAG chondroitin sulfate (CS1), reduce the activity of bone-resorbing osteoclasts, but they also induce functions of the bone-forming cells, the osteoblasts.
26598647	8	44	theme	matrix	1533:1538	arg1	formation					1540:1548	stronger extracellular matrix formation	1510:1548	stronger extracellular matrix formation	1510:1548	GAGs, especially sHA1, were found to severely impact vesicle-extracellular matrix interaction and matrix vesicle activity, leading to stronger extracellular matrix formation and mineralization.
26598647	3	45	theme	previous	425:432	arg1	studies					434:440	previous studies	425:440	previous studies	425:440	In previous studies, we reported that coatings containing GAGs, such as hyaluronic acid (HA) and its synthetically sulfated derivative (sHA1) as well as the naturally low-sulfated GAG chondroitin sulfate (CS1), reduce the activity of bone-resorbing osteoclasts, but they also induce functions of the bone-forming cells, the osteoblasts.
26598647	5	46	theme	CS1	1089:1091	arg1	effects					1064:1070	the molecular effects	1050:1070	the molecular effects of sHA1, HA, and CS1 on MV activity and on the distribution of marker proteins	1050:1149	Here, we studied the molecular effects of sHA1, HA, and CS1 on MV activity and on the distribution of marker proteins.
26598647	6	47	theme	isolated	1249:1256	arg1	MVs					1258:1260	isolated MVs	1249:1260	isolated MVs	1249:1260	Furthermore, we used comparative proteomic methods to study the relative protein compositions of isolated MVs and MV-releasing osteoblasts.
26598647	9	48	theme	molecular	1701:1709	arg1	mechanisms					1711:1720	underlying molecular mechanisms	1690:1720	underlying molecular mechanisms	1690:1720	This study shows that the regulation of MV activity is one important mode of action of GAGs and provides information on underlying molecular mechanisms.
26598647	2	49	theme	native	358:363	arg1	environment					370:380	the native bone environment	354:380	the native bone environment	354:380	One promising therapeutic approach involves the coating of implants with modified glycosaminoglycans (GAGs) that mimic the native bone environment and actively facilitate skeletogenesis.
26598647	0	50	theme	Activity	51:58	arg1	Vesicles					27:34	Osteoblast-released Matrix Vesicles	0:34	Osteoblast-released Matrix Vesicles	0:34	Osteoblast-released Matrix Vesicles, Regulation of Activity and Composition by Sulfated and Non-sulfated Glycosaminoglycans.
26598647	0	50	theme	Activity	51:58	arg1	Regulation					37:46	Regulation	37:46	Regulation of Activity and Composition by Sulfated and Non-sulfated Glycosaminoglycans	37:122	Osteoblast-released Matrix Vesicles, Regulation of Activity and Composition by Sulfated and Non-sulfated Glycosaminoglycans.
26598647	9	51	from	information	1675:1685	arg1	mechanisms					1711:1720	underlying molecular mechanisms	1690:1720	underlying molecular mechanisms	1690:1720	This study shows that the regulation of MV activity is one important mode of action of GAGs and provides information on underlying molecular mechanisms.
26598647	3	52	theme	bone-resorbing	656:669	arg1	osteoclasts					671:681	bone-resorbing osteoclasts	656:681	bone-resorbing osteoclasts	656:681	In previous studies, we reported that coatings containing GAGs, such as hyaluronic acid (HA) and its synthetically sulfated derivative (sHA1) as well as the naturally low-sulfated GAG chondroitin sulfate (CS1), reduce the activity of bone-resorbing osteoclasts, but they also induce functions of the bone-forming cells, the osteoblasts.
26598647	5	53	theme	MV	1096:1097	arg1	activity					1099:1106	MV activity	1096:1106	MV activity	1096:1106	Here, we studied the molecular effects of sHA1, HA, and CS1 on MV activity and on the distribution of marker proteins.
26598647	3	54	theme	osteoclasts	671:681	arg1	activity					644:651	the activity	640:651	the activity of bone-resorbing osteoclasts	640:681	In previous studies, we reported that coatings containing GAGs, such as hyaluronic acid (HA) and its synthetically sulfated derivative (sHA1) as well as the naturally low-sulfated GAG chondroitin sulfate (CS1), reduce the activity of bone-resorbing osteoclasts, but they also induce functions of the bone-forming cells, the osteoblasts.
26598647	7	55	theme	MV	1296:1297	arg1	proteome					1299:1306	The MV proteome	1292:1306	The MV proteome	1292:1306	The MV proteome is much more strongly regulated by GAGs than the cellular proteome.
26598647	2	56	theme	promising	239:247	arg1	approach					261:268	One promising therapeutic approach	235:268	One promising therapeutic approach	235:268	One promising therapeutic approach involves the coating of implants with modified glycosaminoglycans (GAGs) that mimic the native bone environment and actively facilitate skeletogenesis.
26598647	3	57	theme	hyaluronic	494:503	arg1	HA					511:512	HA	511:512	HA	511:512	In previous studies, we reported that coatings containing GAGs, such as hyaluronic acid (HA) and its synthetically sulfated derivative (sHA1) as well as the naturally low-sulfated GAG chondroitin sulfate (CS1), reduce the activity of bone-resorbing osteoclasts, but they also induce functions of the bone-forming cells, the osteoblasts.
26598647	3	57	theme	hyaluronic	494:503	arg1	acid					505:508	hyaluronic acid	494:508	hyaluronic acid (HA)	494:513	In previous studies, we reported that coatings containing GAGs, such as hyaluronic acid (HA) and its synthetically sulfated derivative (sHA1) as well as the naturally low-sulfated GAG chondroitin sulfate (CS1), reduce the activity of bone-resorbing osteoclasts, but they also induce functions of the bone-forming cells, the osteoblasts.
26598647	2	58	with	implants	294:301	arg1	GAGs					337:340	GAGs	337:340	GAGs	337:340	One promising therapeutic approach involves the coating of implants with modified glycosaminoglycans (GAGs) that mimic the native bone environment and actively facilitate skeletogenesis.
26598647	2	58	with	implants	294:301	arg1	glycosaminoglycans					317:334	modified glycosaminoglycans	308:334	modified glycosaminoglycans (GAGs) that mimic the native bone environment and actively facilitate skeletogenesis	308:419	One promising therapeutic approach involves the coating of implants with modified glycosaminoglycans (GAGs) that mimic the native bone environment and actively facilitate skeletogenesis.
26598647	6	59	theme	protein	1225:1231	arg1	compositions					1233:1244	the relative protein compositions	1212:1244	the relative protein compositions of isolated MVs and MV-releasing osteoblasts	1212:1289	Furthermore, we used comparative proteomic methods to study the relative protein compositions of isolated MVs and MV-releasing osteoblasts.
26598647	3	60	contain	containing	469:478	arg1	coatings					460:467	coatings	460:467	coatings containing GAGs	460:483	In previous studies, we reported that coatings containing GAGs, such as hyaluronic acid (HA) and its synthetically sulfated derivative (sHA1) as well as the naturally low-sulfated GAG chondroitin sulfate (CS1), reduce the activity of bone-resorbing osteoclasts, but they also induce functions of the bone-forming cells, the osteoblasts.
26598647	3	60	contain	containing	469:478	arg1	sulfate					618:624	the naturally low-sulfated GAG chondroitin sulfate	575:624	the naturally low-sulfated GAG chondroitin sulfate (CS1)	575:630	In previous studies, we reported that coatings containing GAGs, such as hyaluronic acid (HA) and its synthetically sulfated derivative (sHA1) as well as the naturally low-sulfated GAG chondroitin sulfate (CS1), reduce the activity of bone-resorbing osteoclasts, but they also induce functions of the bone-forming cells, the osteoblasts.
26598647	3	60	contain	containing	469:478	arg1	acid					505:508	hyaluronic acid	494:508	hyaluronic acid (HA)	494:513	In previous studies, we reported that coatings containing GAGs, such as hyaluronic acid (HA) and its synthetically sulfated derivative (sHA1) as well as the naturally low-sulfated GAG chondroitin sulfate (CS1), reduce the activity of bone-resorbing osteoclasts, but they also induce functions of the bone-forming cells, the osteoblasts.
26598647	3	60	contain	containing	469:478	arg2	GAGs					480:483	GAGs	480:483	GAGs	480:483	In previous studies, we reported that coatings containing GAGs, such as hyaluronic acid (HA) and its synthetically sulfated derivative (sHA1) as well as the naturally low-sulfated GAG chondroitin sulfate (CS1), reduce the activity of bone-resorbing osteoclasts, but they also induce functions of the bone-forming cells, the osteoblasts.
26598647	3	60	contain	containing	469:478	arg1	derivative					546:555	its synthetically sulfated derivative	519:555	its synthetically sulfated derivative (sHA1)	519:562	In previous studies, we reported that coatings containing GAGs, such as hyaluronic acid (HA) and its synthetically sulfated derivative (sHA1) as well as the naturally low-sulfated GAG chondroitin sulfate (CS1), reduce the activity of bone-resorbing osteoclasts, but they also induce functions of the bone-forming cells, the osteoblasts.
26598647	6	61	theme	MV-releasing	1266:1277	arg1	osteoblasts					1279:1289	MV-releasing osteoblasts	1266:1289	MV-releasing osteoblasts	1266:1289	Furthermore, we used comparative proteomic methods to study the relative protein compositions of isolated MVs and MV-releasing osteoblasts.
26598647	8	62	theme	matrix	1451:1456	arg1	interaction					1458:1468	vesicle-extracellular matrix interaction	1429:1468	vesicle-extracellular matrix interaction	1429:1468	GAGs, especially sHA1, were found to severely impact vesicle-extracellular matrix interaction and matrix vesicle activity, leading to stronger extracellular matrix formation and mineralization.
26598647	3	63	theme	chondroitin	606:616	arg1	sulfate					618:624	the naturally low-sulfated GAG chondroitin sulfate	575:624	the naturally low-sulfated GAG chondroitin sulfate (CS1)	575:630	In previous studies, we reported that coatings containing GAGs, such as hyaluronic acid (HA) and its synthetically sulfated derivative (sHA1) as well as the naturally low-sulfated GAG chondroitin sulfate (CS1), reduce the activity of bone-resorbing osteoclasts, but they also induce functions of the bone-forming cells, the osteoblasts.
26598647	3	63	theme	chondroitin	606:616	arg1	CS1					627:629	CS1	627:629	CS1	627:629	In previous studies, we reported that coatings containing GAGs, such as hyaluronic acid (HA) and its synthetically sulfated derivative (sHA1) as well as the naturally low-sulfated GAG chondroitin sulfate (CS1), reduce the activity of bone-resorbing osteoclasts, but they also induce functions of the bone-forming cells, the osteoblasts.
26598647	0	64	theme	Composition	64:74	arg1	Vesicles					27:34	Osteoblast-released Matrix Vesicles	0:34	Osteoblast-released Matrix Vesicles	0:34	Osteoblast-released Matrix Vesicles, Regulation of Activity and Composition by Sulfated and Non-sulfated Glycosaminoglycans.
26598647	0	64	theme	Composition	64:74	arg1	Regulation					37:46	Regulation	37:46	Regulation of Activity and Composition by Sulfated and Non-sulfated Glycosaminoglycans	37:122	Osteoblast-released Matrix Vesicles, Regulation of Activity and Composition by Sulfated and Non-sulfated Glycosaminoglycans.
26598647	5	65	theme	sHA1	1075:1078	arg1	effects					1064:1070	the molecular effects	1050:1070	the molecular effects of sHA1, HA, and CS1 on MV activity and on the distribution of marker proteins	1050:1149	Here, we studied the molecular effects of sHA1, HA, and CS1 on MV activity and on the distribution of marker proteins.
26598647	5	66	from	effects	1064:1070	arg1	distribution					1119:1130	the distribution	1115:1130	the distribution of marker proteins	1115:1149	Here, we studied the molecular effects of sHA1, HA, and CS1 on MV activity and on the distribution of marker proteins.
26598647	5	66	from	effects	1064:1070	arg1	activity					1099:1106	MV activity	1096:1106	MV activity	1096:1106	Here, we studied the molecular effects of sHA1, HA, and CS1 on MV activity and on the distribution of marker proteins.
26598647	4	67	theme	bone	1017:1020	arg1	formation					1022:1030	bone formation	1017:1030	bone formation	1017:1030	However, it remained open whether GAGs influence the osteoblasts alone or whether they also directly affect the formation, composition, activity, and distribution of osteoblast-released matrix vesicles (MV), which are supposed to be the active machinery for bone formation.
26598647	8	68	theme	stronger	1510:1517	arg1	formation					1540:1548	stronger extracellular matrix formation	1510:1548	stronger extracellular matrix formation	1510:1548	GAGs, especially sHA1, were found to severely impact vesicle-extracellular matrix interaction and matrix vesicle activity, leading to stronger extracellular matrix formation and mineralization.
28533017	6	0	theme	diverse	780:786	arg1	distribution					798:809	diverse pore size distribution	780:809	diverse pore size distribution	780:809	Scanning Electron Microscopy (SEM) showed porous structures with diverse pore size distribution, which was dependent on the enzyme type and starch source, but no differences were observed in the total granule surface occupied by pores.
28533017	6	1	located	observed	894:901	arg1	surface					924:930	the total granule surface	906:930	the total granule surface occupied by pores	906:948	Scanning Electron Microscopy (SEM) showed porous structures with diverse pore size distribution, which was dependent on the enzyme type and starch source, but no differences were observed in the total granule surface occupied by pores.
28533017	6	1	located	observed	894:901	arg2	differences					877:887	no differences	874:887	no differences	874:887	Scanning Electron Microscopy (SEM) showed porous structures with diverse pore size distribution, which was dependent on the enzyme type and starch source, but no differences were observed in the total granule surface occupied by pores.
28533017	9	2	theme	tuber	1500:1504	arg1	starches					1506:1513	cereal and tuber starches	1489:1513	starches	1506:1513	A heatmap illustrated the diverse pasting properties of the different porous starches, which also showed significant different thermal properties, with different behavior between cereal and tuber starches.
28533017	0	3	theme	botanical	94:102	arg1	sources					104:110	different botanical sources	84:110	different botanical sources	84:110	Morphological and physicochemical characterization of porous starches obtained from different botanical sources and amylolytic enzymes.
28533017	9	4	theme	different	1370:1378	arg1	starches					1387:1394	the different porous starches	1366:1394	the different porous starches	1366:1394	A heatmap illustrated the diverse pasting properties of the different porous starches, which also showed significant different thermal properties, with different behavior between cereal and tuber starches.
28533017	8	5	theme	amorphous	1294:1302	arg1	area					1304:1307	attacked amorphous area	1285:1307	attacked amorphous area	1285:1307	Amylose content analysis revealed different hydrolysis pattern of the amylases, suggesting that AMG mainly affected crystalline region meanwhile AM and CGTase attacked amorphous area.
28533017	10	6	theme	starches	1572:1579	arg1	properties					1558:1567	the properties	1554:1567	the properties of starches	1554:1579	Therefore, it is possible to modulate the properties of starches through the use of different enzymes.
28533017	0	7	theme	amylolytic	116:125	arg1	enzymes					127:133	amylolytic enzymes	116:133	amylolytic enzymes	116:133	Morphological and physicochemical characterization of porous starches obtained from different botanical sources and amylolytic enzymes.
28533017	9	8	theme	porous	1380:1385	arg1	starches					1387:1394	the different porous starches	1366:1394	the different porous starches	1366:1394	A heatmap illustrated the diverse pasting properties of the different porous starches, which also showed significant different thermal properties, with different behavior between cereal and tuber starches.
28533017	7	9	theme	high	1020:1023	arg1	capacity					1042:1049	high water absorptive capacity	1020:1049	high water absorptive capacity	1020:1049	The adsorptive capacity analysis revealed that modified starches had high water absorptive capacity and showed different oil adsorptive capacity depending on the enzyme type.
28533017	6	10	theme	starch	855:860	arg1	source					862:867	starch source	855:867	starch source	855:867	Scanning Electron Microscopy (SEM) showed porous structures with diverse pore size distribution, which was dependent on the enzyme type and starch source, but no differences were observed in the total granule surface occupied by pores.
28533017	7	11	theme	absorptive	1031:1040	arg1	capacity					1042:1049	high water absorptive capacity	1020:1049	high water absorptive capacity	1020:1049	The adsorptive capacity analysis revealed that modified starches had high water absorptive capacity and showed different oil adsorptive capacity depending on the enzyme type.
28533017	3	12	theme	amylolytic	401:410	arg1	enzymes					412:418	different amylolytic enzymes	391:418	different amylolytic enzymes	391:418	Objective was to study the action of different amylolytic enzymes for producing porous starches.
28533017	9	13	theme	starches	1387:1394	arg1	properties					1352:1361	the diverse pasting properties	1332:1361	the diverse pasting properties of the different porous starches, which also showed significant different thermal properties, with different behavior between cereal and tuber starches	1332:1513	A heatmap illustrated the diverse pasting properties of the different porous starches, which also showed significant different thermal properties, with different behavior between cereal and tuber starches.
28533017	5	14	theme	adsorptive	649:658	arg1	capacity					660:667	adsorptive capacity	649:667	adsorptive capacity	649:667	Morphological characteristics, chemical composition, adsorptive capacity and pasting/thermal properties were assessed.
28533017	8	15	theme	crystalline	1242:1252	arg1	meanwhile					1261:1269	crystalline region meanwhile AM	1242:1272	crystalline region meanwhile AM	1242:1272	Amylose content analysis revealed different hydrolysis pattern of the amylases, suggesting that AMG mainly affected crystalline region meanwhile AM and CGTase attacked amorphous area.
28533017	4	16	theme	Wheat	451:455	arg1	starches					483:490	Wheat, rice, potato and cassava starches	451:490	Wheat, rice, potato and cassava starches	451:490	Wheat, rice, potato and cassava starches were treated with Amyloglucosidase (AMG), α-amylase (AM) and cyclodextrin-glycosyltransferase (CGTase).
28533017	7	17	contain	had	1016:1018	arg1	starches					1007:1014	modified starches	998:1014	modified starches	998:1014	The adsorptive capacity analysis revealed that modified starches had high water absorptive capacity and showed different oil adsorptive capacity depending on the enzyme type.
28533017	7	17	contain	had	1016:1018	arg2	capacity					1042:1049	high water absorptive capacity	1020:1049	high water absorptive capacity	1020:1049	The adsorptive capacity analysis revealed that modified starches had high water absorptive capacity and showed different oil adsorptive capacity depending on the enzyme type.
28533017	5	18	theme	pasting/thermal	673:687	arg1	properties					689:698	pasting/thermal properties	673:698	pasting/thermal properties	673:698	Morphological characteristics, chemical composition, adsorptive capacity and pasting/thermal properties were assessed.
28533017	5	19	theme	Morphological	596:608	arg1	characteristics					610:624	Morphological characteristics	596:624	Morphological characteristics	596:624	Morphological characteristics, chemical composition, adsorptive capacity and pasting/thermal properties were assessed.
28533017	0	20	theme	Morphological	0:12	arg1	characterization					34:49	Morphological and physicochemical characterization	0:49	Morphological and physicochemical characterization of porous starches	0:68	Morphological and physicochemical characterization of porous starches obtained from different botanical sources and amylolytic enzymes.
28533017	6	21	theme	porous	757:762	arg1	structures					764:773	porous structures	757:773	porous structures	757:773	Scanning Electron Microscopy (SEM) showed porous structures with diverse pore size distribution, which was dependent on the enzyme type and starch source, but no differences were observed in the total granule surface occupied by pores.
28533017	7	22	theme	modified	998:1005	arg1	starches					1007:1014	modified starches	998:1014	modified starches	998:1014	The adsorptive capacity analysis revealed that modified starches had high water absorptive capacity and showed different oil adsorptive capacity depending on the enzyme type.
28533017	6	23	theme	granule	916:922	arg1	surface					924:930	the total granule surface	906:930	the total granule surface occupied by pores	906:948	Scanning Electron Microscopy (SEM) showed porous structures with diverse pore size distribution, which was dependent on the enzyme type and starch source, but no differences were observed in the total granule surface occupied by pores.
28533017	8	24	theme	Amylose	1126:1132	arg1	analysis					1142:1149	Amylose content analysis	1126:1149	Amylose content analysis	1126:1149	Amylose content analysis revealed different hydrolysis pattern of the amylases, suggesting that AMG mainly affected crystalline region meanwhile AM and CGTase attacked amorphous area.
28533017	6	25	theme	enzyme	839:844	arg1	type					846:849	enzyme type	839:849	enzyme type	839:849	Scanning Electron Microscopy (SEM) showed porous structures with diverse pore size distribution, which was dependent on the enzyme type and starch source, but no differences were observed in the total granule surface occupied by pores.
28533017	10	26	theme	different	1600:1608	arg1	enzymes					1610:1616	different enzymes	1600:1616	different enzymes	1600:1616	Therefore, it is possible to modulate the properties of starches through the use of different enzymes.
28533017	3	27	theme	porous	434:439	arg1	starches					441:448	porous starches	434:448	porous starches	434:448	Objective was to study the action of different amylolytic enzymes for producing porous starches.
28533017	7	28	theme	capacity	966:973	arg1	analysis					975:982	The adsorptive capacity analysis	951:982	The adsorptive capacity analysis	951:982	The adsorptive capacity analysis revealed that modified starches had high water absorptive capacity and showed different oil adsorptive capacity depending on the enzyme type.
28533017	1	29	theme	variety	213:219	arg1	bio-adsorbents					193:206	bio-adsorbents	193:206	bio-adsorbents of a variety of compounds	193:232	Porous starches might offer an attractive alternative as bio-adsorbents of a variety of compounds.
28533017	1	29	theme	variety	213:219	arg1	alternative					178:188	an attractive alternative	164:188	an attractive alternative	164:188	Porous starches might offer an attractive alternative as bio-adsorbents of a variety of compounds.
28533017	3	30	theme	enzymes	412:418	arg1	action					381:386	the action	377:386	the action of different amylolytic enzymes for producing porous starches	377:448	Objective was to study the action of different amylolytic enzymes for producing porous starches.
28533017	9	31	theme	significant	1415:1425	arg1	properties					1445:1454	significant different thermal properties	1415:1454	significant different thermal properties	1415:1454	A heatmap illustrated the diverse pasting properties of the different porous starches, which also showed significant different thermal properties, with different behavior between cereal and tuber starches.
28533017	5	32	theme	chemical	627:634	arg1	composition					636:646	chemical composition	627:646	chemical composition	627:646	Morphological characteristics, chemical composition, adsorptive capacity and pasting/thermal properties were assessed.
28533017	8	33	theme	attacked	1285:1292	arg1	area					1304:1307	attacked amorphous area	1285:1307	attacked amorphous area	1285:1307	Amylose content analysis revealed different hydrolysis pattern of the amylases, suggesting that AMG mainly affected crystalline region meanwhile AM and CGTase attacked amorphous area.
28533017	0	34	theme	physicochemical	18:32	arg1	characterization					34:49	Morphological and physicochemical characterization	0:49	Morphological and physicochemical characterization of porous starches	0:68	Morphological and physicochemical characterization of porous starches obtained from different botanical sources and amylolytic enzymes.
28533017	9	35	theme	different	1427:1435	arg1	properties					1445:1454	significant different thermal properties	1415:1454	significant different thermal properties	1415:1454	A heatmap illustrated the diverse pasting properties of the different porous starches, which also showed significant different thermal properties, with different behavior between cereal and tuber starches.
28533017	7	36	theme	water	1025:1029	arg1	capacity					1042:1049	high water absorptive capacity	1020:1049	high water absorptive capacity	1020:1049	The adsorptive capacity analysis revealed that modified starches had high water absorptive capacity and showed different oil adsorptive capacity depending on the enzyme type.
28533017	6	37	theme	total	910:914	arg1	surface					924:930	the total granule surface	906:930	the total granule surface occupied by pores	906:948	Scanning Electron Microscopy (SEM) showed porous structures with diverse pore size distribution, which was dependent on the enzyme type and starch source, but no differences were observed in the total granule surface occupied by pores.
28533017	1	38	theme	compounds	224:232	arg1	compounds					224:232	compounds	224:232	compounds	224:232	Porous starches might offer an attractive alternative as bio-adsorbents of a variety of compounds.
28533017	1	38	theme	compounds	224:232	arg1	variety					213:219	a variety	211:219	a variety of compounds	211:232	Porous starches might offer an attractive alternative as bio-adsorbents of a variety of compounds.
28533017	0	39	theme	porous	54:59	arg1	starches					61:68	porous starches	54:68	porous starches	54:68	Morphological and physicochemical characterization of porous starches obtained from different botanical sources and amylolytic enzymes.
28533017	7	40	theme	enzyme	1113:1118	arg1	type					1120:1123	the enzyme type	1109:1123	the enzyme type	1109:1123	The adsorptive capacity analysis revealed that modified starches had high water absorptive capacity and showed different oil adsorptive capacity depending on the enzyme type.
28533017	4	41	theme	cassava	475:481	arg1	starches					483:490	Wheat, rice, potato and cassava starches	451:490	Wheat, rice, potato and cassava starches	451:490	Wheat, rice, potato and cassava starches were treated with Amyloglucosidase (AMG), α-amylase (AM) and cyclodextrin-glycosyltransferase (CGTase).
28533017	2	42	theme	physicochemical	259:273	arg1	properties					275:284	physicochemical properties	259:284	physicochemical properties	259:284	However, morphology and physicochemical properties of starches must be understood before exploring their applications.
28533017	6	43	with	structures	764:773	arg1	distribution					798:809	diverse pore size distribution	780:809	diverse pore size distribution	780:809	Scanning Electron Microscopy (SEM) showed porous structures with diverse pore size distribution, which was dependent on the enzyme type and starch source, but no differences were observed in the total granule surface occupied by pores.
28533017	8	44	theme	region	1254:1259	arg1	meanwhile					1261:1269	crystalline region meanwhile AM	1242:1272	crystalline region meanwhile AM	1242:1272	Amylose content analysis revealed different hydrolysis pattern of the amylases, suggesting that AMG mainly affected crystalline region meanwhile AM and CGTase attacked amorphous area.
28533017	7	45	theme	oil	1072:1074	arg1	capacity					1087:1094	different oil adsorptive capacity	1062:1094	different oil adsorptive capacity	1062:1094	The adsorptive capacity analysis revealed that modified starches had high water absorptive capacity and showed different oil adsorptive capacity depending on the enzyme type.
28533017	3	46	theme	different	391:399	arg1	enzymes					412:418	different amylolytic enzymes	391:418	different amylolytic enzymes	391:418	Objective was to study the action of different amylolytic enzymes for producing porous starches.
28533017	10	47	theme	enzymes	1610:1616	arg1	use					1593:1595	the use	1589:1595	the use of different enzymes	1589:1616	Therefore, it is possible to modulate the properties of starches through the use of different enzymes.
28533017	7	48	theme	adsorptive	1076:1085	arg1	capacity					1087:1094	different oil adsorptive capacity	1062:1094	different oil adsorptive capacity	1062:1094	The adsorptive capacity analysis revealed that modified starches had high water absorptive capacity and showed different oil adsorptive capacity depending on the enzyme type.
28533017	1	49	theme	Porous	136:141	arg1	starches					143:150	Porous starches	136:150	Porous starches	136:150	Porous starches might offer an attractive alternative as bio-adsorbents of a variety of compounds.
28533017	0	50	theme	starches	61:68	arg1	characterization					34:49	Morphological and physicochemical characterization	0:49	Morphological and physicochemical characterization of porous starches	0:68	Morphological and physicochemical characterization of porous starches obtained from different botanical sources and amylolytic enzymes.
28533017	8	51	theme	hydrolysis	1170:1179	arg1	pattern					1181:1187	different hydrolysis pattern	1160:1187	different hydrolysis pattern of the amylases	1160:1203	Amylose content analysis revealed different hydrolysis pattern of the amylases, suggesting that AMG mainly affected crystalline region meanwhile AM and CGTase attacked amorphous area.
28533017	9	52	theme	diverse	1336:1342	arg1	properties					1352:1361	the diverse pasting properties	1332:1361	the diverse pasting properties of the different porous starches, which also showed significant different thermal properties, with different behavior between cereal and tuber starches	1332:1513	A heatmap illustrated the diverse pasting properties of the different porous starches, which also showed significant different thermal properties, with different behavior between cereal and tuber starches.
28533017	6	53	theme	Electron	724:731	arg1	SEM					745:747	SEM	745:747	SEM	745:747	Scanning Electron Microscopy (SEM) showed porous structures with diverse pore size distribution, which was dependent on the enzyme type and starch source, but no differences were observed in the total granule surface occupied by pores.
28533017	6	53	theme	Electron	724:731	arg1	Microscopy					733:742	Scanning Electron Microscopy	715:742	Scanning Electron Microscopy (SEM)	715:748	Scanning Electron Microscopy (SEM) showed porous structures with diverse pore size distribution, which was dependent on the enzyme type and starch source, but no differences were observed in the total granule surface occupied by pores.
28533017	9	54	theme	different	1462:1470	arg1	behavior					1472:1479	different behavior	1462:1479	different behavior between cereal and tuber starches	1462:1513	A heatmap illustrated the diverse pasting properties of the different porous starches, which also showed significant different thermal properties, with different behavior between cereal and tuber starches.
28533017	8	55	theme	different	1160:1168	arg1	pattern					1181:1187	different hydrolysis pattern	1160:1187	different hydrolysis pattern of the amylases	1160:1203	Amylose content analysis revealed different hydrolysis pattern of the amylases, suggesting that AMG mainly affected crystalline region meanwhile AM and CGTase attacked amorphous area.
28533017	4	56	theme	rice	458:461	arg1	starches					483:490	Wheat, rice, potato and cassava starches	451:490	Wheat, rice, potato and cassava starches	451:490	Wheat, rice, potato and cassava starches were treated with Amyloglucosidase (AMG), α-amylase (AM) and cyclodextrin-glycosyltransferase (CGTase).
28533017	7	57	theme	different	1062:1070	arg1	capacity					1087:1094	different oil adsorptive capacity	1062:1094	different oil adsorptive capacity	1062:1094	The adsorptive capacity analysis revealed that modified starches had high water absorptive capacity and showed different oil adsorptive capacity depending on the enzyme type.
28533017	6	58	theme	Scanning	715:722	arg1	SEM					745:747	SEM	745:747	SEM	745:747	Scanning Electron Microscopy (SEM) showed porous structures with diverse pore size distribution, which was dependent on the enzyme type and starch source, but no differences were observed in the total granule surface occupied by pores.
28533017	6	58	theme	Scanning	715:722	arg1	Microscopy					733:742	Scanning Electron Microscopy	715:742	Scanning Electron Microscopy (SEM)	715:748	Scanning Electron Microscopy (SEM) showed porous structures with diverse pore size distribution, which was dependent on the enzyme type and starch source, but no differences were observed in the total granule surface occupied by pores.
28533017	8	59	theme	content	1134:1140	arg1	analysis					1142:1149	Amylose content analysis	1126:1149	Amylose content analysis	1126:1149	Amylose content analysis revealed different hydrolysis pattern of the amylases, suggesting that AMG mainly affected crystalline region meanwhile AM and CGTase attacked amorphous area.
28533017	8	60	dep	meanwhile	1261:1269	arg1	area					1304:1307	attacked amorphous area	1285:1307	attacked amorphous area	1285:1307	Amylose content analysis revealed different hydrolysis pattern of the amylases, suggesting that AMG mainly affected crystalline region meanwhile AM and CGTase attacked amorphous area.
28533017	9	61	theme	pasting	1344:1350	arg1	properties					1352:1361	the diverse pasting properties	1332:1361	the diverse pasting properties of the different porous starches, which also showed significant different thermal properties, with different behavior between cereal and tuber starches	1332:1513	A heatmap illustrated the diverse pasting properties of the different porous starches, which also showed significant different thermal properties, with different behavior between cereal and tuber starches.
28533017	2	62	theme	starches	289:296	arg1	morphology					244:253	morphology	244:253	morphology	244:253	However, morphology and physicochemical properties of starches must be understood before exploring their applications.
28533017	2	62	theme	starches	289:296	arg1	properties					275:284	physicochemical properties	259:284	physicochemical properties	259:284	However, morphology and physicochemical properties of starches must be understood before exploring their applications.
28533017	6	63	dep	type	846:849	arg1	the					835:837	the	835:837	the	835:837	Scanning Electron Microscopy (SEM) showed porous structures with diverse pore size distribution, which was dependent on the enzyme type and starch source, but no differences were observed in the total granule surface occupied by pores.
28533017	0	64	theme	different	84:92	arg1	sources					104:110	different botanical sources	84:110	different botanical sources	84:110	Morphological and physicochemical characterization of porous starches obtained from different botanical sources and amylolytic enzymes.
28533017	4	65	theme	potato	464:469	arg1	starches					483:490	Wheat, rice, potato and cassava starches	451:490	Wheat, rice, potato and cassava starches	451:490	Wheat, rice, potato and cassava starches were treated with Amyloglucosidase (AMG), α-amylase (AM) and cyclodextrin-glycosyltransferase (CGTase).
28533017	6	66	theme	size	793:796	arg1	distribution					798:809	diverse pore size distribution	780:809	diverse pore size distribution	780:809	Scanning Electron Microscopy (SEM) showed porous structures with diverse pore size distribution, which was dependent on the enzyme type and starch source, but no differences were observed in the total granule surface occupied by pores.
28533017	7	67	theme	adsorptive	955:964	arg1	capacity					966:973	The adsorptive capacity	951:973	The adsorptive capacity analysis	951:982	The adsorptive capacity analysis revealed that modified starches had high water absorptive capacity and showed different oil adsorptive capacity depending on the enzyme type.
28533017	9	68	theme	cereal	1489:1494	arg1	starches					1506:1513	cereal and tuber starches	1489:1513	starches	1506:1513	A heatmap illustrated the diverse pasting properties of the different porous starches, which also showed significant different thermal properties, with different behavior between cereal and tuber starches.
28533017	8	69	theme	amylases	1196:1203	arg1	pattern					1181:1187	different hydrolysis pattern	1160:1187	different hydrolysis pattern of the amylases	1160:1203	Amylose content analysis revealed different hydrolysis pattern of the amylases, suggesting that AMG mainly affected crystalline region meanwhile AM and CGTase attacked amorphous area.
28533017	9	70	dep	different	1427:1435	arg1	thermal					1437:1443	thermal	1437:1443	thermal	1437:1443	A heatmap illustrated the diverse pasting properties of the different porous starches, which also showed significant different thermal properties, with different behavior between cereal and tuber starches.
28533017	6	71	theme	pore	788:791	arg1	distribution					798:809	diverse pore size distribution	780:809	diverse pore size distribution	780:809	Scanning Electron Microscopy (SEM) showed porous structures with diverse pore size distribution, which was dependent on the enzyme type and starch source, but no differences were observed in the total granule surface occupied by pores.
28533017	1	72	theme	attractive	167:176	arg1	bio-adsorbents					193:206	bio-adsorbents	193:206	bio-adsorbents of a variety of compounds	193:232	Porous starches might offer an attractive alternative as bio-adsorbents of a variety of compounds.
28533017	1	72	theme	attractive	167:176	arg1	alternative					178:188	an attractive alternative	164:188	an attractive alternative	164:188	Porous starches might offer an attractive alternative as bio-adsorbents of a variety of compounds.
28929976	4	0	theme	coefficients	681:692	arg1	values					671:676	high values	666:676	high values of coefficients of determination (>0·97)	666:717	The variance analysis shows high values of coefficients of determination (>0·97) while no significant lack of fit was evident.
28929976	1	1	theme	β-galactosidase	161:175	arg1	dose					184:187	β-galactosidase enzyme dose	161:187	β-galactosidase enzyme dose	161:187	The experiments reported in this research paper describe the effects of β-galactosidase enzyme dose and cheese whey amount, on the maximum concentration and yield of galacto-oligosaccahride (GOS) and reaction time.
28929976	5	2	theme	products	861:868	arg1	manufacture					846:856	the manufacture	842:856	the manufacture of products enriched in bioactive compounds with high value-added	842:922	Hence, the models could be employed to select reaction conditions applied in the manufacture of products enriched in bioactive compounds with high value-added.
28929976	1	3	theme	enzyme	177:182	arg1	dose					184:187	β-galactosidase enzyme dose	161:187	β-galactosidase enzyme dose	161:187	The experiments reported in this research paper describe the effects of β-galactosidase enzyme dose and cheese whey amount, on the maximum concentration and yield of galacto-oligosaccahride (GOS) and reaction time.
28929976	3	4	dep	concentration	523:535	arg1	the					519:521	the	519:521	the	519:521	The results indicate that the proposed mathematical models could adequately describe the concentration and yield of GOS and the reaction time within the limits of the factors that are being investigated.
28929976	1	5	theme	galacto-oligosaccahride	255:277	arg1	yield					246:250	yield	246:250	yield	246:250	The experiments reported in this research paper describe the effects of β-galactosidase enzyme dose and cheese whey amount, on the maximum concentration and yield of galacto-oligosaccahride (GOS) and reaction time.
28929976	1	5	theme	galacto-oligosaccahride	255:277	arg1	concentration					228:240	maximum concentration	220:240	maximum concentration	220:240	The experiments reported in this research paper describe the effects of β-galactosidase enzyme dose and cheese whey amount, on the maximum concentration and yield of galacto-oligosaccahride (GOS) and reaction time.
28929976	1	6	from	effects	150:156	arg1	yield					246:250	yield	246:250	yield	246:250	The experiments reported in this research paper describe the effects of β-galactosidase enzyme dose and cheese whey amount, on the maximum concentration and yield of galacto-oligosaccahride (GOS) and reaction time.
28929976	1	6	from	effects	150:156	arg1	concentration					228:240	maximum concentration	220:240	maximum concentration	220:240	The experiments reported in this research paper describe the effects of β-galactosidase enzyme dose and cheese whey amount, on the maximum concentration and yield of galacto-oligosaccahride (GOS) and reaction time.
28929976	2	7	theme	rotational	357:366	arg1	CCRD					376:379	CCRD	376:379	CCRD	376:379	The experimental plan was based on central composite rotational design (CCRD) and modelled by response surface methodology (RSM).
28929976	2	7	theme	rotational	357:366	arg1	design					368:373	central composite rotational design	339:373	central composite rotational design (CCRD)	339:380	The experimental plan was based on central composite rotational design (CCRD) and modelled by response surface methodology (RSM).
28929976	1	8	theme	research	122:129	arg1	paper					131:135	this research paper	117:135	this research paper	117:135	The experiments reported in this research paper describe the effects of β-galactosidase enzyme dose and cheese whey amount, on the maximum concentration and yield of galacto-oligosaccahride (GOS) and reaction time.
28929976	5	9	with	compounds	892:900	arg1	value-added					912:922	value-added	912:922	value-added	912:922	Hence, the models could be employed to select reaction conditions applied in the manufacture of products enriched in bioactive compounds with high value-added.
28929976	1	10	theme	dose	184:187	arg1	effects					150:156	the effects	146:156	the effects	146:156	The experiments reported in this research paper describe the effects of β-galactosidase enzyme dose and cheese whey amount, on the maximum concentration and yield of galacto-oligosaccahride (GOS) and reaction time.
28929976	4	11	theme	fit	748:750	arg1	lack					740:743	no significant lack	725:743	no significant lack of fit	725:750	The variance analysis shows high values of coefficients of determination (>0·97) while no significant lack of fit was evident.
28929976	3	12	theme	mathematical	473:484	arg1	models					486:491	the proposed mathematical models	460:491	the proposed mathematical models	460:491	The results indicate that the proposed mathematical models could adequately describe the concentration and yield of GOS and the reaction time within the limits of the factors that are being investigated.
28929976	0	13	theme	surface	9:15	arg1	methodology					17:27	Response surface methodology	0:27	Response surface methodology	0:27	Response surface methodology as a tool for modelling galacto-oligosaccharide production.
28929976	1	14	theme	cheese	193:198	arg1	amount					205:210	cheese whey amount	193:210	cheese whey amount	193:210	The experiments reported in this research paper describe the effects of β-galactosidase enzyme dose and cheese whey amount, on the maximum concentration and yield of galacto-oligosaccahride (GOS) and reaction time.
28929976	0	15	theme	Response	0:7	arg1	methodology					17:27	Response surface methodology	0:27	Response surface methodology	0:27	Response surface methodology as a tool for modelling galacto-oligosaccharide production.
28929976	2	16	theme	experimental	308:319	arg1	plan					321:324	The experimental plan	304:324	The experimental plan	304:324	The experimental plan was based on central composite rotational design (CCRD) and modelled by response surface methodology (RSM).
28929976	1	17	theme	whey	200:203	arg1	amount					205:210	cheese whey amount	193:210	cheese whey amount	193:210	The experiments reported in this research paper describe the effects of β-galactosidase enzyme dose and cheese whey amount, on the maximum concentration and yield of galacto-oligosaccahride (GOS) and reaction time.
28929976	3	18	theme	GOS	550:552	arg1	yield					541:545	yield	541:545	yield	541:545	The results indicate that the proposed mathematical models could adequately describe the concentration and yield of GOS and the reaction time within the limits of the factors that are being investigated.
28929976	3	18	theme	GOS	550:552	arg1	concentration					523:535	concentration	523:535	concentration	523:535	The results indicate that the proposed mathematical models could adequately describe the concentration and yield of GOS and the reaction time within the limits of the factors that are being investigated.
28929976	3	18	theme	GOS	550:552	arg1	time					571:574	the reaction time	558:574	the reaction time	558:574	The results indicate that the proposed mathematical models could adequately describe the concentration and yield of GOS and the reaction time within the limits of the factors that are being investigated.
28929976	1	19	theme	amount	205:210	arg1	effects					150:156	the effects	146:156	the effects	146:156	The experiments reported in this research paper describe the effects of β-galactosidase enzyme dose and cheese whey amount, on the maximum concentration and yield of galacto-oligosaccahride (GOS) and reaction time.
28929976	3	20	theme	reaction	562:569	arg1	time					571:574	the reaction time	558:574	the reaction time	558:574	The results indicate that the proposed mathematical models could adequately describe the concentration and yield of GOS and the reaction time within the limits of the factors that are being investigated.
28929976	1	21	theme	reaction	289:296	arg1	time					298:301	reaction time	289:301	reaction time	289:301	The experiments reported in this research paper describe the effects of β-galactosidase enzyme dose and cheese whey amount, on the maximum concentration and yield of galacto-oligosaccahride (GOS) and reaction time.
28929976	2	22	theme	composite	347:355	arg1	CCRD					376:379	CCRD	376:379	CCRD	376:379	The experimental plan was based on central composite rotational design (CCRD) and modelled by response surface methodology (RSM).
28929976	2	22	theme	composite	347:355	arg1	design					368:373	central composite rotational design	339:373	central composite rotational design (CCRD)	339:380	The experimental plan was based on central composite rotational design (CCRD) and modelled by response surface methodology (RSM).
28929976	2	23	theme	surface	407:413	arg1	RSM					428:430	RSM	428:430	RSM	428:430	The experimental plan was based on central composite rotational design (CCRD) and modelled by response surface methodology (RSM).
28929976	2	23	theme	surface	407:413	arg1	methodology					415:425	response surface methodology	398:425	response surface methodology (RSM)	398:431	The experimental plan was based on central composite rotational design (CCRD) and modelled by response surface methodology (RSM).
28929976	1	24	theme	time	298:301	arg1	yield					246:250	yield	246:250	yield	246:250	The experiments reported in this research paper describe the effects of β-galactosidase enzyme dose and cheese whey amount, on the maximum concentration and yield of galacto-oligosaccahride (GOS) and reaction time.
28929976	1	24	theme	time	298:301	arg1	concentration					228:240	maximum concentration	220:240	maximum concentration	220:240	The experiments reported in this research paper describe the effects of β-galactosidase enzyme dose and cheese whey amount, on the maximum concentration and yield of galacto-oligosaccahride (GOS) and reaction time.
28929976	2	25	theme	response	398:405	arg1	RSM					428:430	RSM	428:430	RSM	428:430	The experimental plan was based on central composite rotational design (CCRD) and modelled by response surface methodology (RSM).
28929976	2	25	theme	response	398:405	arg1	methodology					415:425	response surface methodology	398:425	response surface methodology (RSM)	398:431	The experimental plan was based on central composite rotational design (CCRD) and modelled by response surface methodology (RSM).
28929976	4	26	theme	variance	642:649	arg1	analysis					651:658	The variance analysis	638:658	The variance analysis	638:658	The variance analysis shows high values of coefficients of determination (>0·97) while no significant lack of fit was evident.
28929976	5	27	theme	reaction	811:818	arg1	conditions					820:829	reaction conditions	811:829	reaction conditions applied in the manufacture of products enriched in bioactive compounds with high value-added	811:922	Hence, the models could be employed to select reaction conditions applied in the manufacture of products enriched in bioactive compounds with high value-added.
28929976	1	28	dep	concentration	228:240	arg1	the					216:218	the	216:218	the	216:218	The experiments reported in this research paper describe the effects of β-galactosidase enzyme dose and cheese whey amount, on the maximum concentration and yield of galacto-oligosaccahride (GOS) and reaction time.
28929976	2	29	theme	central	339:345	arg1	CCRD					376:379	CCRD	376:379	CCRD	376:379	The experimental plan was based on central composite rotational design (CCRD) and modelled by response surface methodology (RSM).
28929976	2	29	theme	central	339:345	arg1	design					368:373	central composite rotational design	339:373	central composite rotational design (CCRD)	339:380	The experimental plan was based on central composite rotational design (CCRD) and modelled by response surface methodology (RSM).
28929976	5	30	theme	bioactive	882:890	arg1	compounds					892:900	bioactive compounds	882:900	bioactive compounds with high value-added	882:922	Hence, the models could be employed to select reaction conditions applied in the manufacture of products enriched in bioactive compounds with high value-added.
28929976	4	31	theme	determination	697:709	arg1	coefficients					681:692	coefficients	681:692	coefficients of determination (>0·97)	681:717	The variance analysis shows high values of coefficients of determination (>0·97) while no significant lack of fit was evident.
28929976	3	32	theme	factors	601:607	arg1	limits					587:592	the limits	583:592	the limits of the factors that are being investigated	583:635	The results indicate that the proposed mathematical models could adequately describe the concentration and yield of GOS and the reaction time within the limits of the factors that are being investigated.
28929976	4	33	theme	significant	728:738	arg1	lack					740:743	no significant lack	725:743	no significant lack of fit	725:750	The variance analysis shows high values of coefficients of determination (>0·97) while no significant lack of fit was evident.
28929976	1	34	theme	maximum	220:226	arg1	concentration					228:240	maximum concentration	220:240	maximum concentration	220:240	The experiments reported in this research paper describe the effects of β-galactosidase enzyme dose and cheese whey amount, on the maximum concentration and yield of galacto-oligosaccahride (GOS) and reaction time.
28929976	0	35	dep	methodology	17:27	arg1	tool					34:37	a tool	32:37	a tool for modelling galacto-oligosaccharide production	32:86	Response surface methodology as a tool for modelling galacto-oligosaccharide production.
28929976	3	36	theme	proposed	464:471	arg1	models					486:491	the proposed mathematical models	460:491	the proposed mathematical models	460:491	The results indicate that the proposed mathematical models could adequately describe the concentration and yield of GOS and the reaction time within the limits of the factors that are being investigated.
28929976	0	37	theme	galacto-oligosaccharide	53:75	arg1	production					77:86	galacto-oligosaccharide production	53:86	galacto-oligosaccharide production	53:86	Response surface methodology as a tool for modelling galacto-oligosaccharide production.
28929976	4	38	theme	high	666:669	arg1	values					671:676	high values	666:676	high values of coefficients of determination (>0·97)	666:717	The variance analysis shows high values of coefficients of determination (>0·97) while no significant lack of fit was evident.
27287105	3	0	theme	moisture	703:710	arg1	absorbance					712:721	lower moisture absorbance	697:721	lower moisture absorbance	697:721	Chitosan films neutralized in citrate buffer showed greater physical integrity resulted from greater thicknesses, lower moisture absorbance, lower tendency to solubility in the acid medium, and better swelling capacities.
27287105	7	1	dep	presence	1410:1417	arg1	the					1406:1408	the	1406:1408	the	1406:1408	The results suggest that the neutralized chitosan citrate film prepared without glycerol promotes a cosmetic effect for skin exfoliation in the presence or absence of hyaluronic acid.
27287105	5	2	theme	hyaluronic	1098:1107	arg1	release					1114:1120	hyaluronic acid release	1098:1120	hyaluronic acid release in a skin model	1098:1136	Since the composition prepared in citrate buffer without glycerol was considered to present better physical integrity, it was applied to investigate hyaluronic acid release in a skin model.
27287105	2	3	from	analysis	535:542	arg1	medium					506:511	acid medium	501:511	acid medium	501:511	To choose the appropriate film composition for this study, neutralized chitosan citrate and acetate films, with and without the plasticizer glycerol, were prepared and characterized by thickness, moisture content, degree of swelling, total soluble matter in acid medium, simultaneous thermal analysis and differential scanning calorimetry.
27287105	0	4	theme	cosmetic	74:81	arg1	application					83:93	cosmetic application	74:93	cosmetic application	74:93	Films based on neutralized chitosan citrate as innovative composition for cosmetic application.
27287105	2	5	from	thickness	428:436	arg1	medium					506:511	acid medium	501:511	acid medium	501:511	To choose the appropriate film composition for this study, neutralized chitosan citrate and acetate films, with and without the plasticizer glycerol, were prepared and characterized by thickness, moisture content, degree of swelling, total soluble matter in acid medium, simultaneous thermal analysis and differential scanning calorimetry.
27287105	4	6	theme	higher	852:857	arg1	interaction					859:869	higher interaction	852:869	higher interaction	852:869	According to thermal analyses, these films had higher interaction with water which is considered an important feature for cosmetic application.
27287105	2	7	theme	acetate	335:341	arg1	films					343:347	acetate films	335:347	acetate films	335:347	To choose the appropriate film composition for this study, neutralized chitosan citrate and acetate films, with and without the plasticizer glycerol, were prepared and characterized by thickness, moisture content, degree of swelling, total soluble matter in acid medium, simultaneous thermal analysis and differential scanning calorimetry.
27287105	5	8	theme	citrate	983:989	arg1	buffer					991:996	citrate buffer	983:996	citrate buffer without glycerol	983:1013	Since the composition prepared in citrate buffer without glycerol was considered to present better physical integrity, it was applied to investigate hyaluronic acid release in a skin model.
27287105	7	9	theme	cosmetic	1366:1373	arg1	effect					1375:1380	a cosmetic effect	1364:1380	a cosmetic effect for skin exfoliation	1364:1401	The results suggest that the neutralized chitosan citrate film prepared without glycerol promotes a cosmetic effect for skin exfoliation in the presence or absence of hyaluronic acid.
27287105	6	10	theme	stratum	1209:1215	arg1	corneum					1217:1223	stratum corneum desquamation and hydration	1209:1250	corneum	1217:1223	Skins treated with those films, with or without hyaluronic acid, show stratum corneum desquamation and hydration within 10min.
27287105	7	11	theme	hyaluronic	1433:1442	arg1	acid					1444:1447	hyaluronic acid	1433:1447	hyaluronic acid	1433:1447	The results suggest that the neutralized chitosan citrate film prepared without glycerol promotes a cosmetic effect for skin exfoliation in the presence or absence of hyaluronic acid.
27287105	3	12	theme	lower	724:728	arg1	tendency					730:737	lower tendency	724:737	lower tendency to solubility in the acid medium	724:770	Chitosan films neutralized in citrate buffer showed greater physical integrity resulted from greater thicknesses, lower moisture absorbance, lower tendency to solubility in the acid medium, and better swelling capacities.
27287105	2	13	theme	scanning	561:568	arg1	calorimetry					570:580	differential scanning calorimetry	548:580	differential scanning calorimetry	548:580	To choose the appropriate film composition for this study, neutralized chitosan citrate and acetate films, with and without the plasticizer glycerol, were prepared and characterized by thickness, moisture content, degree of swelling, total soluble matter in acid medium, simultaneous thermal analysis and differential scanning calorimetry.
27287105	2	14	theme	film	269:272	arg1	composition					274:284	the appropriate film composition	253:284	the appropriate film composition	253:284	To choose the appropriate film composition for this study, neutralized chitosan citrate and acetate films, with and without the plasticizer glycerol, were prepared and characterized by thickness, moisture content, degree of swelling, total soluble matter in acid medium, simultaneous thermal analysis and differential scanning calorimetry.
27287105	2	15	theme	soluble	483:489	arg1	matter					491:496	total soluble matter	477:496	total soluble matter in acid medium	477:511	To choose the appropriate film composition for this study, neutralized chitosan citrate and acetate films, with and without the plasticizer glycerol, were prepared and characterized by thickness, moisture content, degree of swelling, total soluble matter in acid medium, simultaneous thermal analysis and differential scanning calorimetry.
27287105	5	16	theme	acid	1109:1112	arg1	release					1114:1120	hyaluronic acid release	1098:1120	hyaluronic acid release in a skin model	1098:1136	Since the composition prepared in citrate buffer without glycerol was considered to present better physical integrity, it was applied to investigate hyaluronic acid release in a skin model.
27287105	2	17	theme	appropriate	257:267	arg1	composition					274:284	the appropriate film composition	253:284	the appropriate film composition	253:284	To choose the appropriate film composition for this study, neutralized chitosan citrate and acetate films, with and without the plasticizer glycerol, were prepared and characterized by thickness, moisture content, degree of swelling, total soluble matter in acid medium, simultaneous thermal analysis and differential scanning calorimetry.
27287105	7	18	theme	citrate	1316:1322	arg1	film					1324:1327	the neutralized chitosan citrate film	1291:1327	the neutralized chitosan citrate film prepared without glycerol	1291:1353	The results suggest that the neutralized chitosan citrate film prepared without glycerol promotes a cosmetic effect for skin exfoliation in the presence or absence of hyaluronic acid.
27287105	2	19	theme	plasticizer	371:381	arg1	glycerol					383:390	the plasticizer glycerol	367:390	the plasticizer glycerol	367:390	To choose the appropriate film composition for this study, neutralized chitosan citrate and acetate films, with and without the plasticizer glycerol, were prepared and characterized by thickness, moisture content, degree of swelling, total soluble matter in acid medium, simultaneous thermal analysis and differential scanning calorimetry.
27287105	2	20	theme	total	477:481	arg1	matter					491:496	total soluble matter	477:496	total soluble matter in acid medium	477:511	To choose the appropriate film composition for this study, neutralized chitosan citrate and acetate films, with and without the plasticizer glycerol, were prepared and characterized by thickness, moisture content, degree of swelling, total soluble matter in acid medium, simultaneous thermal analysis and differential scanning calorimetry.
27287105	3	21	dep	showed	628:633	arg1	resulted					662:669	resulted	662:669	showed greater physical integrity resulted from greater thicknesses, lower moisture absorbance, lower tendency to solubility in the acid medium, and better swelling capacities	628:802	Chitosan films neutralized in citrate buffer showed greater physical integrity resulted from greater thicknesses, lower moisture absorbance, lower tendency to solubility in the acid medium, and better swelling capacities.
27287105	3	22	theme	physical	643:650	arg1	integrity					652:660	greater physical integrity	635:660	greater physical integrity	635:660	Chitosan films neutralized in citrate buffer showed greater physical integrity resulted from greater thicknesses, lower moisture absorbance, lower tendency to solubility in the acid medium, and better swelling capacities.
27287105	2	23	from	content	448:454	arg1	medium					506:511	acid medium	501:511	acid medium	501:511	To choose the appropriate film composition for this study, neutralized chitosan citrate and acetate films, with and without the plasticizer glycerol, were prepared and characterized by thickness, moisture content, degree of swelling, total soluble matter in acid medium, simultaneous thermal analysis and differential scanning calorimetry.
27287105	2	24	dep	choose	246:251	arg1	neutralized					302:312	neutralized	302:312	neutralized chitosan citrate and acetate films	302:347	To choose the appropriate film composition for this study, neutralized chitosan citrate and acetate films, with and without the plasticizer glycerol, were prepared and characterized by thickness, moisture content, degree of swelling, total soluble matter in acid medium, simultaneous thermal analysis and differential scanning calorimetry.
27287105	2	25	from	calorimetry	570:580	arg1	medium					506:511	acid medium	501:511	acid medium	501:511	To choose the appropriate film composition for this study, neutralized chitosan citrate and acetate films, with and without the plasticizer glycerol, were prepared and characterized by thickness, moisture content, degree of swelling, total soluble matter in acid medium, simultaneous thermal analysis and differential scanning calorimetry.
27287105	1	26	theme	chitosan	176:183	arg1	salts					185:189	chitosan salts	176:189	chitosan salts	176:189	In this work, citrate and acetate buffers, were investigated as neutralizers to chitosan salts in order to provide biocompatible and stable films.
27287105	2	27	theme	simultaneous	514:525	arg1	analysis					535:542	simultaneous thermal analysis	514:542	simultaneous thermal analysis	514:542	To choose the appropriate film composition for this study, neutralized chitosan citrate and acetate films, with and without the plasticizer glycerol, were prepared and characterized by thickness, moisture content, degree of swelling, total soluble matter in acid medium, simultaneous thermal analysis and differential scanning calorimetry.
27287105	5	28	theme	better	1041:1046	arg1	integrity					1057:1065	better physical integrity	1041:1065	better physical integrity	1041:1065	Since the composition prepared in citrate buffer without glycerol was considered to present better physical integrity, it was applied to investigate hyaluronic acid release in a skin model.
27287105	7	29	theme	neutralized	1295:1305	arg1	film					1324:1327	the neutralized chitosan citrate film	1291:1327	the neutralized chitosan citrate film prepared without glycerol	1291:1353	The results suggest that the neutralized chitosan citrate film prepared without glycerol promotes a cosmetic effect for skin exfoliation in the presence or absence of hyaluronic acid.
27287105	3	30	from	tendency	730:737	arg1	medium					765:770	the acid medium	756:770	the acid medium	756:770	Chitosan films neutralized in citrate buffer showed greater physical integrity resulted from greater thicknesses, lower moisture absorbance, lower tendency to solubility in the acid medium, and better swelling capacities.
27287105	5	31	theme	skin	1127:1130	arg1	model					1132:1136	a skin model	1125:1136	a skin model	1125:1136	Since the composition prepared in citrate buffer without glycerol was considered to present better physical integrity, it was applied to investigate hyaluronic acid release in a skin model.
27287105	4	32	theme	important	905:913	arg1	feature					915:921	an important feature	902:921	an important feature for cosmetic application	902:946	According to thermal analyses, these films had higher interaction with water which is considered an important feature for cosmetic application.
27287105	3	33	theme	greater	635:641	arg1	integrity					652:660	greater physical integrity	635:660	greater physical integrity	635:660	Chitosan films neutralized in citrate buffer showed greater physical integrity resulted from greater thicknesses, lower moisture absorbance, lower tendency to solubility in the acid medium, and better swelling capacities.
27287105	6	34	dep	corneum	1217:1223	arg1	desquamation					1225:1236	desquamation	1225:1236	desquamation	1225:1236	Skins treated with those films, with or without hyaluronic acid, show stratum corneum desquamation and hydration within 10min.
27287105	7	35	theme	chitosan	1307:1314	arg1	film					1324:1327	the neutralized chitosan citrate film	1291:1327	the neutralized chitosan citrate film prepared without glycerol	1291:1353	The results suggest that the neutralized chitosan citrate film prepared without glycerol promotes a cosmetic effect for skin exfoliation in the presence or absence of hyaluronic acid.
27287105	3	36	from	thicknesses	684:694	arg1	medium					765:770	the acid medium	756:770	the acid medium	756:770	Chitosan films neutralized in citrate buffer showed greater physical integrity resulted from greater thicknesses, lower moisture absorbance, lower tendency to solubility in the acid medium, and better swelling capacities.
27287105	4	37	theme	cosmetic	927:934	arg1	application					936:946	cosmetic application	927:946	cosmetic application	927:946	According to thermal analyses, these films had higher interaction with water which is considered an important feature for cosmetic application.
27287105	2	38	from	degree	457:462	arg1	medium					506:511	acid medium	501:511	acid medium	501:511	To choose the appropriate film composition for this study, neutralized chitosan citrate and acetate films, with and without the plasticizer glycerol, were prepared and characterized by thickness, moisture content, degree of swelling, total soluble matter in acid medium, simultaneous thermal analysis and differential scanning calorimetry.
27287105	7	39	theme	skin	1386:1389	arg1	exfoliation					1391:1401	skin exfoliation	1386:1401	skin exfoliation	1386:1401	The results suggest that the neutralized chitosan citrate film prepared without glycerol promotes a cosmetic effect for skin exfoliation in the presence or absence of hyaluronic acid.
27287105	7	40	theme	acid	1444:1447	arg1	presence					1410:1417	presence	1410:1417	presence	1410:1417	The results suggest that the neutralized chitosan citrate film prepared without glycerol promotes a cosmetic effect for skin exfoliation in the presence or absence of hyaluronic acid.
27287105	7	40	theme	acid	1444:1447	arg1	absence					1422:1428	absence	1422:1428	absence	1422:1428	The results suggest that the neutralized chitosan citrate film prepared without glycerol promotes a cosmetic effect for skin exfoliation in the presence or absence of hyaluronic acid.
27287105	2	41	theme	acid	501:504	arg1	medium					506:511	acid medium	501:511	acid medium	501:511	To choose the appropriate film composition for this study, neutralized chitosan citrate and acetate films, with and without the plasticizer glycerol, were prepared and characterized by thickness, moisture content, degree of swelling, total soluble matter in acid medium, simultaneous thermal analysis and differential scanning calorimetry.
27287105	3	42	theme	Chitosan	583:590	arg1	films					592:596	Chitosan films	583:596	Chitosan films neutralized in citrate buffer	583:626	Chitosan films neutralized in citrate buffer showed greater physical integrity resulted from greater thicknesses, lower moisture absorbance, lower tendency to solubility in the acid medium, and better swelling capacities.
27287105	0	43	theme	innovative	47:56	arg1	composition					58:68	innovative composition	47:68	innovative composition for cosmetic application	47:93	Films based on neutralized chitosan citrate as innovative composition for cosmetic application.
27287105	2	44	theme	differential	548:559	arg1	calorimetry					570:580	differential scanning calorimetry	548:580	differential scanning calorimetry	548:580	To choose the appropriate film composition for this study, neutralized chitosan citrate and acetate films, with and without the plasticizer glycerol, were prepared and characterized by thickness, moisture content, degree of swelling, total soluble matter in acid medium, simultaneous thermal analysis and differential scanning calorimetry.
27287105	2	45	theme	swelling	467:474	arg1	matter					491:496	total soluble matter	477:496	total soluble matter in acid medium	477:511	To choose the appropriate film composition for this study, neutralized chitosan citrate and acetate films, with and without the plasticizer glycerol, were prepared and characterized by thickness, moisture content, degree of swelling, total soluble matter in acid medium, simultaneous thermal analysis and differential scanning calorimetry.
27287105	2	45	theme	swelling	467:474	arg1	analysis					535:542	simultaneous thermal analysis	514:542	simultaneous thermal analysis	514:542	To choose the appropriate film composition for this study, neutralized chitosan citrate and acetate films, with and without the plasticizer glycerol, were prepared and characterized by thickness, moisture content, degree of swelling, total soluble matter in acid medium, simultaneous thermal analysis and differential scanning calorimetry.
27287105	2	45	theme	swelling	467:474	arg1	degree					457:462	degree	457:462	degree of swelling	457:474	To choose the appropriate film composition for this study, neutralized chitosan citrate and acetate films, with and without the plasticizer glycerol, were prepared and characterized by thickness, moisture content, degree of swelling, total soluble matter in acid medium, simultaneous thermal analysis and differential scanning calorimetry.
27287105	2	45	theme	swelling	467:474	arg1	thickness					428:436	thickness	428:436	thickness	428:436	To choose the appropriate film composition for this study, neutralized chitosan citrate and acetate films, with and without the plasticizer glycerol, were prepared and characterized by thickness, moisture content, degree of swelling, total soluble matter in acid medium, simultaneous thermal analysis and differential scanning calorimetry.
27287105	2	45	theme	swelling	467:474	arg1	content					448:454	moisture content	439:454	moisture content	439:454	To choose the appropriate film composition for this study, neutralized chitosan citrate and acetate films, with and without the plasticizer glycerol, were prepared and characterized by thickness, moisture content, degree of swelling, total soluble matter in acid medium, simultaneous thermal analysis and differential scanning calorimetry.
27287105	2	45	theme	swelling	467:474	arg1	calorimetry					570:580	differential scanning calorimetry	548:580	differential scanning calorimetry	548:580	To choose the appropriate film composition for this study, neutralized chitosan citrate and acetate films, with and without the plasticizer glycerol, were prepared and characterized by thickness, moisture content, degree of swelling, total soluble matter in acid medium, simultaneous thermal analysis and differential scanning calorimetry.
27287105	2	46	dep	prepared	398:405	arg1	choose					246:251	choose	246:251	choose	246:251	To choose the appropriate film composition for this study, neutralized chitosan citrate and acetate films, with and without the plasticizer glycerol, were prepared and characterized by thickness, moisture content, degree of swelling, total soluble matter in acid medium, simultaneous thermal analysis and differential scanning calorimetry.
27287105	3	47	theme	acid	760:763	arg1	medium					765:770	the acid medium	756:770	the acid medium	756:770	Chitosan films neutralized in citrate buffer showed greater physical integrity resulted from greater thicknesses, lower moisture absorbance, lower tendency to solubility in the acid medium, and better swelling capacities.
27287105	5	48	theme	physical	1048:1055	arg1	integrity					1057:1065	better physical integrity	1041:1065	better physical integrity	1041:1065	Since the composition prepared in citrate buffer without glycerol was considered to present better physical integrity, it was applied to investigate hyaluronic acid release in a skin model.
27287105	4	49	theme	thermal	818:824	arg1	analyses					826:833	thermal analyses	818:833	thermal analyses	818:833	According to thermal analyses, these films had higher interaction with water which is considered an important feature for cosmetic application.
27287105	5	50	from	release	1114:1120	arg1	model					1132:1136	a skin model	1125:1136	a skin model	1125:1136	Since the composition prepared in citrate buffer without glycerol was considered to present better physical integrity, it was applied to investigate hyaluronic acid release in a skin model.
27287105	3	51	from	capacities	793:802	arg1	medium					765:770	the acid medium	756:770	the acid medium	756:770	Chitosan films neutralized in citrate buffer showed greater physical integrity resulted from greater thicknesses, lower moisture absorbance, lower tendency to solubility in the acid medium, and better swelling capacities.
27287105	3	52	theme	greater	676:682	arg1	thicknesses					684:694	greater thicknesses	676:694	greater thicknesses	676:694	Chitosan films neutralized in citrate buffer showed greater physical integrity resulted from greater thicknesses, lower moisture absorbance, lower tendency to solubility in the acid medium, and better swelling capacities.
27287105	2	53	theme	chitosan	314:321	arg1	citrate					323:329	chitosan citrate	314:329	chitosan citrate	314:329	To choose the appropriate film composition for this study, neutralized chitosan citrate and acetate films, with and without the plasticizer glycerol, were prepared and characterized by thickness, moisture content, degree of swelling, total soluble matter in acid medium, simultaneous thermal analysis and differential scanning calorimetry.
27287105	4	54	contain	had	848:850	arg1	films					842:846	these films	836:846	these films	836:846	According to thermal analyses, these films had higher interaction with water which is considered an important feature for cosmetic application.
27287105	4	54	contain	had	848:850	arg2	interaction					859:869	higher interaction	852:869	higher interaction	852:869	According to thermal analyses, these films had higher interaction with water which is considered an important feature for cosmetic application.
27287105	2	55	theme	thermal	527:533	arg1	analysis					535:542	simultaneous thermal analysis	514:542	simultaneous thermal analysis	514:542	To choose the appropriate film composition for this study, neutralized chitosan citrate and acetate films, with and without the plasticizer glycerol, were prepared and characterized by thickness, moisture content, degree of swelling, total soluble matter in acid medium, simultaneous thermal analysis and differential scanning calorimetry.
27287105	3	56	theme	citrate	613:619	arg1	buffer					621:626	citrate buffer	613:626	citrate buffer	613:626	Chitosan films neutralized in citrate buffer showed greater physical integrity resulted from greater thicknesses, lower moisture absorbance, lower tendency to solubility in the acid medium, and better swelling capacities.
27287105	1	57	theme	acetate	122:128	arg1	neutralizers					160:171	neutralizers	160:171	neutralizers to chitosan salts	160:189	In this work, citrate and acetate buffers, were investigated as neutralizers to chitosan salts in order to provide biocompatible and stable films.
27287105	1	57	theme	acetate	122:128	arg1	buffers					130:136	acetate buffers	122:136	acetate buffers	122:136	In this work, citrate and acetate buffers, were investigated as neutralizers to chitosan salts in order to provide biocompatible and stable films.
27287105	1	57	theme	acetate	122:128	arg1	citrate					110:116	citrate	110:116	citrate	110:116	In this work, citrate and acetate buffers, were investigated as neutralizers to chitosan salts in order to provide biocompatible and stable films.
27287105	1	58	theme	biocompatible	211:223	arg1	films					236:240	biocompatible and stable films	211:240	biocompatible and stable films	211:240	In this work, citrate and acetate buffers, were investigated as neutralizers to chitosan salts in order to provide biocompatible and stable films.
27287105	2	59	from	matter	491:496	arg1	medium					506:511	acid medium	501:511	acid medium	501:511	To choose the appropriate film composition for this study, neutralized chitosan citrate and acetate films, with and without the plasticizer glycerol, were prepared and characterized by thickness, moisture content, degree of swelling, total soluble matter in acid medium, simultaneous thermal analysis and differential scanning calorimetry.
27287105	6	60	theme	hyaluronic	1187:1196	arg1	acid					1198:1201	hyaluronic acid	1187:1201	hyaluronic acid	1187:1201	Skins treated with those films, with or without hyaluronic acid, show stratum corneum desquamation and hydration within 10min.
27287105	3	61	theme	better	777:782	arg1	capacities					793:802	better swelling capacities	777:802	better swelling capacities	777:802	Chitosan films neutralized in citrate buffer showed greater physical integrity resulted from greater thicknesses, lower moisture absorbance, lower tendency to solubility in the acid medium, and better swelling capacities.
27287105	2	62	theme	moisture	439:446	arg1	content					448:454	moisture content	439:454	moisture content	439:454	To choose the appropriate film composition for this study, neutralized chitosan citrate and acetate films, with and without the plasticizer glycerol, were prepared and characterized by thickness, moisture content, degree of swelling, total soluble matter in acid medium, simultaneous thermal analysis and differential scanning calorimetry.
27287105	3	63	theme	lower	697:701	arg1	absorbance					712:721	lower moisture absorbance	697:721	lower moisture absorbance	697:721	Chitosan films neutralized in citrate buffer showed greater physical integrity resulted from greater thicknesses, lower moisture absorbance, lower tendency to solubility in the acid medium, and better swelling capacities.
27287105	3	64	theme	swelling	784:791	arg1	capacities					793:802	better swelling capacities	777:802	better swelling capacities	777:802	Chitosan films neutralized in citrate buffer showed greater physical integrity resulted from greater thicknesses, lower moisture absorbance, lower tendency to solubility in the acid medium, and better swelling capacities.
27287105	3	65	from	absorbance	712:721	arg1	medium					765:770	the acid medium	756:770	the acid medium	756:770	Chitosan films neutralized in citrate buffer showed greater physical integrity resulted from greater thicknesses, lower moisture absorbance, lower tendency to solubility in the acid medium, and better swelling capacities.
27287105	1	66	theme	stable	229:234	arg1	films					236:240	biocompatible and stable films	211:240	biocompatible and stable films	211:240	In this work, citrate and acetate buffers, were investigated as neutralizers to chitosan salts in order to provide biocompatible and stable films.
28376366	7	0	dep	decreased	1166:1174	arg1	increasing					1137:1146	increasing	1137:1146	increasing HA concentration	1137:1163	Results showed that increasing HA concentration, decreased gelation time, increased degradation rate, and reduced changes that were observed over time in mechanics, water retention, and crystallization.
28376366	8	1	theme	relevant	1369:1376	arg1	system					1378:1383	a biologically relevant system	1354:1383	a biologically relevant system with controllable temporal stiffening and elasticity	1354:1436	These hydrogel composites provide a biologically relevant system with controllable temporal stiffening and elasticity, thus offering enhanced tunable scaffolds for short or long term applications in tissue engineering.
28376366	7	2	theme	gelation	1176:1183	arg1	time					1185:1188	gelation time	1176:1188	gelation time	1176:1188	Results showed that increasing HA concentration, decreased gelation time, increased degradation rate, and reduced changes that were observed over time in mechanics, water retention, and crystallization.
28376366	7	3	located	observed	1249:1256	arg1	retention					1288:1296	water retention	1282:1296	water retention	1282:1296	Results showed that increasing HA concentration, decreased gelation time, increased degradation rate, and reduced changes that were observed over time in mechanics, water retention, and crystallization.
28376366	7	3	located	observed	1249:1256	arg2	changes					1231:1237	changes	1231:1237	changes that were observed over time in mechanics, water retention, and crystallization	1231:1317	Results showed that increasing HA concentration, decreased gelation time, increased degradation rate, and reduced changes that were observed over time in mechanics, water retention, and crystallization.
28376366	7	3	located	observed	1249:1256	arg1	crystallization					1303:1317	crystallization	1303:1317	crystallization	1303:1317	Results showed that increasing HA concentration, decreased gelation time, increased degradation rate, and reduced changes that were observed over time in mechanics, water retention, and crystallization.
28376366	7	3	located	observed	1249:1256	arg1	mechanics					1271:1279	mechanics	1271:1279	mechanics	1271:1279	Results showed that increasing HA concentration, decreased gelation time, increased degradation rate, and reduced changes that were observed over time in mechanics, water retention, and crystallization.
28376366	1	4	theme	biocompatible	150:162	arg1	hydrogels					174:182	biocompatible composite hydrogels	150:182	biocompatible composite hydrogels	150:182	In this study, silk fibroin and hyaluronic acid (HA) were enzymatically crosslinked to form biocompatible composite hydrogels with tunable mechanical properties similar to that of native tissues.
28376366	8	5	theme	long	1493:1496	arg1	applications					1503:1514	short or long term applications	1484:1514	short or long term applications	1484:1514	These hydrogel composites provide a biologically relevant system with controllable temporal stiffening and elasticity, thus offering enhanced tunable scaffolds for short or long term applications in tissue engineering.
28376366	2	6	theme	silk	302:305	arg1	proteins					315:322	silk fibroin proteins	302:322	silk fibroin proteins via horseradish peroxidase	302:349	The formation of di-tyrosine crosslinks between silk fibroin proteins via horseradish peroxidase has resulted in a highly elastic hydrogel but exhibits time-dependent stiffening related to silk self-assembly and crystallization.
28376366	3	7	theme	limited	610:616	arg1	mechanics					618:626	limited mechanics	610:626	limited mechanics	610:626	Utilizing the same method of crosslinking, tyramine-substituted HA forms hydrophilic and bioactive hydrogels that tend to have limited mechanics and degrade rapidly.
28376366	6	8	theme	enzymatic	1073:1081	arg1	degradation					1083:1093	enzymatic degradation	1073:1093	enzymatic degradation	1073:1093	In addition, the hydrogels were characterized by enzymatic degradation and for cytotoxicity.
28376366	0	9	link	crosslinked	14:24	arg1	acid					42:45	Enzymatically crosslinked silk-hyaluronic acid	0:45	Enzymatically crosslinked silk-hyaluronic acid	0:45	Enzymatically crosslinked silk-hyaluronic acid hydrogels.
28376366	8	10	theme	enhanced	1453:1460	arg1	scaffolds					1470:1478	enhanced tunable scaffolds	1453:1478	enhanced tunable scaffolds for short or long term applications in tissue engineering	1453:1536	These hydrogel composites provide a biologically relevant system with controllable temporal stiffening and elasticity, thus offering enhanced tunable scaffolds for short or long term applications in tissue engineering.
28376366	4	11	theme	composite	766:774	arg1	hydrogel					776:783	a composite hydrogel	764:783	a composite hydrogel that exhibited both mechanical integrity and hydrophilicity	764:843	To address the limitations of these singular component scaffolds, HA was covalently crosslinked with silk, forming a composite hydrogel that exhibited both mechanical integrity and hydrophilicity.
28376366	1	12	attach	crosslinked	130:140	arg3	study					66:70	this study	61:70	this study	61:70	In this study, silk fibroin and hyaluronic acid (HA) were enzymatically crosslinked to form biocompatible composite hydrogels with tunable mechanical properties similar to that of native tissues.
28376366	5	13	theme	infrared	917:924	arg1	spectroscopy					926:937	infrared spectroscopy	917:937	infrared spectroscopy	917:937	The composite hydrogels were assessed using unconfined compression and infrared spectroscopy to reveal of the physical properties over time in relation to polymer concentration.
28376366	1	14	theme	silk	73:76	arg1	fibroin					78:84	silk fibroin	73:84	silk fibroin	73:84	In this study, silk fibroin and hyaluronic acid (HA) were enzymatically crosslinked to form biocompatible composite hydrogels with tunable mechanical properties similar to that of native tissues.
28376366	1	15	theme	composite	164:172	arg1	hydrogels					174:182	biocompatible composite hydrogels	150:182	biocompatible composite hydrogels	150:182	In this study, silk fibroin and hyaluronic acid (HA) were enzymatically crosslinked to form biocompatible composite hydrogels with tunable mechanical properties similar to that of native tissues.
28376366	7	16	theme	water	1282:1286	arg1	retention					1288:1296	water retention	1282:1296	water retention	1282:1296	Results showed that increasing HA concentration, decreased gelation time, increased degradation rate, and reduced changes that were observed over time in mechanics, water retention, and crystallization.
28376366	7	17	theme	degradation	1201:1211	arg1	rate					1213:1216	degradation rate	1201:1216	degradation rate	1201:1216	Results showed that increasing HA concentration, decreased gelation time, increased degradation rate, and reduced changes that were observed over time in mechanics, water retention, and crystallization.
28376366	2	18	theme	di-tyrosine	271:281	arg1	crosslinks					283:292	di-tyrosine crosslinks	271:292	di-tyrosine crosslinks	271:292	The formation of di-tyrosine crosslinks between silk fibroin proteins via horseradish peroxidase has resulted in a highly elastic hydrogel but exhibits time-dependent stiffening related to silk self-assembly and crystallization.
28376366	2	19	theme	time-dependent	406:419	arg1	stiffening					421:430	time-dependent stiffening	406:430	time-dependent stiffening related to silk self-assembly and crystallization	406:480	The formation of di-tyrosine crosslinks between silk fibroin proteins via horseradish peroxidase has resulted in a highly elastic hydrogel but exhibits time-dependent stiffening related to silk self-assembly and crystallization.
28376366	0	20	theme	crosslinked	14:24	arg1	acid					42:45	Enzymatically crosslinked silk-hyaluronic acid	0:45	Enzymatically crosslinked silk-hyaluronic acid	0:45	Enzymatically crosslinked silk-hyaluronic acid hydrogels.
28376366	8	21	from	scaffolds	1470:1478	arg1	engineering					1526:1536	tissue engineering	1519:1536	tissue engineering	1519:1536	These hydrogel composites provide a biologically relevant system with controllable temporal stiffening and elasticity, thus offering enhanced tunable scaffolds for short or long term applications in tissue engineering.
28376366	2	22	theme	horseradish	328:338	arg1	peroxidase					340:349	horseradish peroxidase	328:349	horseradish peroxidase	328:349	The formation of di-tyrosine crosslinks between silk fibroin proteins via horseradish peroxidase has resulted in a highly elastic hydrogel but exhibits time-dependent stiffening related to silk self-assembly and crystallization.
28376366	8	23	with	system	1378:1383	arg1	stiffening					1412:1421	controllable temporal stiffening	1390:1421	controllable temporal stiffening	1390:1421	These hydrogel composites provide a biologically relevant system with controllable temporal stiffening and elasticity, thus offering enhanced tunable scaffolds for short or long term applications in tissue engineering.
28376366	8	23	with	system	1378:1383	arg1	elasticity					1427:1436	elasticity	1427:1436	elasticity	1427:1436	These hydrogel composites provide a biologically relevant system with controllable temporal stiffening and elasticity, thus offering enhanced tunable scaffolds for short or long term applications in tissue engineering.
28376366	1	24	theme	hyaluronic	90:99	arg1	HA					107:108	HA	107:108	HA	107:108	In this study, silk fibroin and hyaluronic acid (HA) were enzymatically crosslinked to form biocompatible composite hydrogels with tunable mechanical properties similar to that of native tissues.
28376366	1	24	theme	hyaluronic	90:99	arg1	acid					101:104	hyaluronic acid	90:104	hyaluronic acid (HA)	90:109	In this study, silk fibroin and hyaluronic acid (HA) were enzymatically crosslinked to form biocompatible composite hydrogels with tunable mechanical properties similar to that of native tissues.
28376366	1	25	theme	tunable	189:195	arg1	properties					208:217	tunable mechanical properties	189:217	tunable mechanical properties similar to that of native tissues	189:251	In this study, silk fibroin and hyaluronic acid (HA) were enzymatically crosslinked to form biocompatible composite hydrogels with tunable mechanical properties similar to that of native tissues.
28376366	5	26	theme	unconfined	890:899	arg1	compression					901:911	unconfined compression	890:911	unconfined compression	890:911	The composite hydrogels were assessed using unconfined compression and infrared spectroscopy to reveal of the physical properties over time in relation to polymer concentration.
28376366	3	27	contain	have	605:608	arg1	hydrogels					582:590	hydrophilic and bioactive hydrogels	556:590	hydrophilic and bioactive hydrogels that tend to have limited mechanics and degrade rapidly	556:646	Utilizing the same method of crosslinking, tyramine-substituted HA forms hydrophilic and bioactive hydrogels that tend to have limited mechanics and degrade rapidly.
28376366	3	27	contain	have	605:608	arg2	mechanics					618:626	limited mechanics	610:626	limited mechanics	610:626	Utilizing the same method of crosslinking, tyramine-substituted HA forms hydrophilic and bioactive hydrogels that tend to have limited mechanics and degrade rapidly.
28376366	4	28	theme	mechanical	805:814	arg1	integrity					816:824	mechanical integrity	805:824	mechanical integrity	805:824	To address the limitations of these singular component scaffolds, HA was covalently crosslinked with silk, forming a composite hydrogel that exhibited both mechanical integrity and hydrophilicity.
28376366	1	29	theme	mechanical	197:206	arg1	properties					208:217	tunable mechanical properties	189:217	tunable mechanical properties similar to that of native tissues	189:251	In this study, silk fibroin and hyaluronic acid (HA) were enzymatically crosslinked to form biocompatible composite hydrogels with tunable mechanical properties similar to that of native tissues.
28376366	0	30	theme	silk-hyaluronic	26:40	arg1	acid					42:45	Enzymatically crosslinked silk-hyaluronic acid	0:45	Enzymatically crosslinked silk-hyaluronic acid	0:45	Enzymatically crosslinked silk-hyaluronic acid hydrogels.
28376366	2	31	theme	crosslinks	283:292	arg1	formation					258:266	The formation	254:266	The formation of di-tyrosine crosslinks between silk fibroin proteins via horseradish peroxidase	254:349	The formation of di-tyrosine crosslinks between silk fibroin proteins via horseradish peroxidase has resulted in a highly elastic hydrogel but exhibits time-dependent stiffening related to silk self-assembly and crystallization.
28376366	2	32	theme	fibroin	307:313	arg1	proteins					315:322	silk fibroin proteins	302:322	silk fibroin proteins via horseradish peroxidase	302:349	The formation of di-tyrosine crosslinks between silk fibroin proteins via horseradish peroxidase has resulted in a highly elastic hydrogel but exhibits time-dependent stiffening related to silk self-assembly and crystallization.
28376366	2	33	theme	silk	443:446	arg1	self-assembly					448:460	silk self-assembly	443:460	silk self-assembly	443:460	The formation of di-tyrosine crosslinks between silk fibroin proteins via horseradish peroxidase has resulted in a highly elastic hydrogel but exhibits time-dependent stiffening related to silk self-assembly and crystallization.
28376366	4	34	theme	scaffolds	704:712	arg1	limitations					664:674	the limitations	660:674	the limitations of these singular component scaffolds	660:712	To address the limitations of these singular component scaffolds, HA was covalently crosslinked with silk, forming a composite hydrogel that exhibited both mechanical integrity and hydrophilicity.
28376366	4	35	theme	component	694:702	arg1	scaffolds					704:712	these singular component scaffolds	679:712	these singular component scaffolds	679:712	To address the limitations of these singular component scaffolds, HA was covalently crosslinked with silk, forming a composite hydrogel that exhibited both mechanical integrity and hydrophilicity.
28376366	5	36	theme	physical	956:963	arg1	properties					965:974	the physical properties	952:974	the physical properties	952:974	The composite hydrogels were assessed using unconfined compression and infrared spectroscopy to reveal of the physical properties over time in relation to polymer concentration.
28376366	1	37	theme	similar	219:225	arg1	properties					208:217	tunable mechanical properties	189:217	tunable mechanical properties similar to that of native tissues	189:251	In this study, silk fibroin and hyaluronic acid (HA) were enzymatically crosslinked to form biocompatible composite hydrogels with tunable mechanical properties similar to that of native tissues.
28376366	5	38	theme	composite	850:858	arg1	hydrogels					860:868	The composite hydrogels	846:868	The composite hydrogels	846:868	The composite hydrogels were assessed using unconfined compression and infrared spectroscopy to reveal of the physical properties over time in relation to polymer concentration.
28376366	2	39	theme	related	432:438	arg1	stiffening					421:430	time-dependent stiffening	406:430	time-dependent stiffening related to silk self-assembly and crystallization	406:480	The formation of di-tyrosine crosslinks between silk fibroin proteins via horseradish peroxidase has resulted in a highly elastic hydrogel but exhibits time-dependent stiffening related to silk self-assembly and crystallization.
28376366	8	40	theme	temporal	1403:1410	arg1	stiffening					1412:1421	controllable temporal stiffening	1390:1421	controllable temporal stiffening	1390:1421	These hydrogel composites provide a biologically relevant system with controllable temporal stiffening and elasticity, thus offering enhanced tunable scaffolds for short or long term applications in tissue engineering.
28376366	8	41	theme	short	1484:1488	arg1	applications					1503:1514	short or long term applications	1484:1514	short or long term applications	1484:1514	These hydrogel composites provide a biologically relevant system with controllable temporal stiffening and elasticity, thus offering enhanced tunable scaffolds for short or long term applications in tissue engineering.
28376366	3	42	theme	tyramine-substituted	526:545	arg1	HA					547:548	tyramine-substituted HA	526:548	tyramine-substituted HA	526:548	Utilizing the same method of crosslinking, tyramine-substituted HA forms hydrophilic and bioactive hydrogels that tend to have limited mechanics and degrade rapidly.
28376366	7	43	theme	HA	1148:1149	arg1	concentration					1151:1163	HA concentration	1148:1163	HA concentration	1148:1163	Results showed that increasing HA concentration, decreased gelation time, increased degradation rate, and reduced changes that were observed over time in mechanics, water retention, and crystallization.
28376366	8	44	theme	tissue	1519:1524	arg1	engineering					1526:1536	tissue engineering	1519:1536	tissue engineering	1519:1536	These hydrogel composites provide a biologically relevant system with controllable temporal stiffening and elasticity, thus offering enhanced tunable scaffolds for short or long term applications in tissue engineering.
28376366	3	45	theme	hydrophilic	556:566	arg1	hydrogels					582:590	hydrophilic and bioactive hydrogels	556:590	hydrophilic and bioactive hydrogels that tend to have limited mechanics and degrade rapidly	556:646	Utilizing the same method of crosslinking, tyramine-substituted HA forms hydrophilic and bioactive hydrogels that tend to have limited mechanics and degrade rapidly.
28376366	8	46	theme	controllable	1390:1401	arg1	stiffening					1412:1421	controllable temporal stiffening	1390:1421	controllable temporal stiffening	1390:1421	These hydrogel composites provide a biologically relevant system with controllable temporal stiffening and elasticity, thus offering enhanced tunable scaffolds for short or long term applications in tissue engineering.
28376366	3	47	theme	crosslinking	512:523	arg1	method					502:507	the same method	493:507	the same method of crosslinking	493:523	Utilizing the same method of crosslinking, tyramine-substituted HA forms hydrophilic and bioactive hydrogels that tend to have limited mechanics and degrade rapidly.
28376366	8	48	theme	term	1498:1501	arg1	applications					1503:1514	short or long term applications	1484:1514	short or long term applications	1484:1514	These hydrogel composites provide a biologically relevant system with controllable temporal stiffening and elasticity, thus offering enhanced tunable scaffolds for short or long term applications in tissue engineering.
28376366	8	49	theme	tunable	1462:1468	arg1	scaffolds					1470:1478	enhanced tunable scaffolds	1453:1478	enhanced tunable scaffolds for short or long term applications in tissue engineering	1453:1536	These hydrogel composites provide a biologically relevant system with controllable temporal stiffening and elasticity, thus offering enhanced tunable scaffolds for short or long term applications in tissue engineering.
28376366	3	50	theme	bioactive	572:580	arg1	hydrogels					582:590	hydrophilic and bioactive hydrogels	556:590	hydrophilic and bioactive hydrogels that tend to have limited mechanics and degrade rapidly	556:646	Utilizing the same method of crosslinking, tyramine-substituted HA forms hydrophilic and bioactive hydrogels that tend to have limited mechanics and degrade rapidly.
28376366	5	51	theme	polymer	1001:1007	arg1	concentration					1009:1021	polymer concentration	1001:1021	polymer concentration	1001:1021	The composite hydrogels were assessed using unconfined compression and infrared spectroscopy to reveal of the physical properties over time in relation to polymer concentration.
28376366	3	52	theme	same	497:500	arg1	method					502:507	the same method	493:507	the same method of crosslinking	493:523	Utilizing the same method of crosslinking, tyramine-substituted HA forms hydrophilic and bioactive hydrogels that tend to have limited mechanics and degrade rapidly.
28376366	4	53	theme	singular	685:692	arg1	scaffolds					704:712	these singular component scaffolds	679:712	these singular component scaffolds	679:712	To address the limitations of these singular component scaffolds, HA was covalently crosslinked with silk, forming a composite hydrogel that exhibited both mechanical integrity and hydrophilicity.
28376366	8	54	theme	hydrogel	1326:1333	arg1	composites					1335:1344	These hydrogel composites	1320:1344	These hydrogel composites	1320:1344	These hydrogel composites provide a biologically relevant system with controllable temporal stiffening and elasticity, thus offering enhanced tunable scaffolds for short or long term applications in tissue engineering.
28376366	1	55	theme	native	238:243	arg1	tissues					245:251	native tissues	238:251	native tissues	238:251	In this study, silk fibroin and hyaluronic acid (HA) were enzymatically crosslinked to form biocompatible composite hydrogels with tunable mechanical properties similar to that of native tissues.
28376366	2	56	theme	elastic	376:382	arg1	hydrogel					384:391	a highly elastic hydrogel	367:391	a highly elastic hydrogel	367:391	The formation of di-tyrosine crosslinks between silk fibroin proteins via horseradish peroxidase has resulted in a highly elastic hydrogel but exhibits time-dependent stiffening related to silk self-assembly and crystallization.
25852230	0	0	theme	suis	76:79	arg1	serotype					81:88	Streptococcus suis serotype 14	62:91	Streptococcus suis serotype 14	62:91	Role of the capsular polysaccharide as a virulence factor for Streptococcus suis serotype 14.
25852230	3	1	theme	devoid	447:452	arg1	mutants					439:445	knockout mutants	430:445	knockout mutants devoid of either cps14B, a highly conserved regulatory gene, or neu14C, a gene coding for uridine diphospho-N-acetylglucosamine 2-epimerase, which is involved in sialic acid synthesis	430:629	To study the role of the capsular polysaccharide (CPS) of serotype 14 as a virulence factor, we constructed knockout mutants devoid of either cps14B, a highly conserved regulatory gene, or neu14C, a gene coding for uridine diphospho-N-acetylglucosamine 2-epimerase, which is involved in sialic acid synthesis.
25852230	0	2	theme	Streptococcus	62:74	arg1	serotype					81:88	Streptococcus suis serotype 14	62:91	Streptococcus suis serotype 14	62:91	Role of the capsular polysaccharide as a virulence factor for Streptococcus suis serotype 14.
25852230	7	3	theme	CPS	924:926	arg1	composition					928:938	the CPS composition	920:938	the CPS composition of different serotypes	920:961	Despite the difference in the CPS composition of different serotypes, this study has demonstrated for the first time that the CPS of a serotype other than 2 is also an important antiphagocytic factor and a critical virulence factor.
25852230	6	4	theme	attenuated	840:849	arg1	virulence					851:859	attenuated virulence	840:859	attenuated virulence of this non-encapsulated mutant	840:891	An in vivo murine model was used to demonstrate attenuated virulence of this non-encapsulated mutant.
25852230	3	5	theme	conserved	481:489	arg1	gene					502:505	a highly conserved regulatory gene	472:505	a highly conserved regulatory gene	472:505	To study the role of the capsular polysaccharide (CPS) of serotype 14 as a virulence factor, we constructed knockout mutants devoid of either cps14B, a highly conserved regulatory gene, or neu14C, a gene coding for uridine diphospho-N-acetylglucosamine 2-epimerase, which is involved in sialic acid synthesis.
25852230	3	5	theme	conserved	481:489	arg1	cps14B					464:469	cps14B	464:469	cps14B	464:469	To study the role of the capsular polysaccharide (CPS) of serotype 14 as a virulence factor, we constructed knockout mutants devoid of either cps14B, a highly conserved regulatory gene, or neu14C, a gene coding for uridine diphospho-N-acetylglucosamine 2-epimerase, which is involved in sialic acid synthesis.
25852230	4	6	theme	total	651:655	arg1	loss					657:660	total loss	651:660	total loss of the CPS	651:671	The mutants showed total loss of the CPS with coagglutination assays and electron microscopy.
25852230	7	7	theme	serotype	1029:1036	arg1	factor					1087:1092	an important antiphagocytic factor	1059:1092	an important antiphagocytic factor	1059:1092	Despite the difference in the CPS composition of different serotypes, this study has demonstrated for the first time that the CPS of a serotype other than 2 is also an important antiphagocytic factor and a critical virulence factor.
25852230	7	7	theme	serotype	1029:1036	arg1	CPS					1020:1022	the CPS	1016:1022	the CPS of a serotype other than 2	1016:1049	Despite the difference in the CPS composition of different serotypes, this study has demonstrated for the first time that the CPS of a serotype other than 2 is also an important antiphagocytic factor and a critical virulence factor.
25852230	1	8	theme	zoonotic	150:157	arg1	agent					159:163	a zoonotic agent	148:163	a zoonotic agent causing meningitis and septicemia	148:197	Streptococcus suis is an important swine pathogen and a zoonotic agent causing meningitis and septicemia.
25852230	7	9	theme	other	1038:1042	arg1	serotype					1029:1036	a serotype	1027:1036	a serotype other than 2	1027:1049	Despite the difference in the CPS composition of different serotypes, this study has demonstrated for the first time that the CPS of a serotype other than 2 is also an important antiphagocytic factor and a critical virulence factor.
25852230	4	10	theme	electron	705:712	arg1	microscopy					714:723	electron microscopy	705:723	electron microscopy	705:723	The mutants showed total loss of the CPS with coagglutination assays and electron microscopy.
25852230	5	11	theme	high	753:756	arg1	susceptibility					758:771	high susceptibility	753:771	high susceptibility of mutant Δcps14B	753:789	Phagocytosis assays showed high susceptibility of mutant Δcps14B.
25852230	4	12	theme	CPS	669:671	arg1	loss					657:660	total loss	651:660	total loss of the CPS	651:671	The mutants showed total loss of the CPS with coagglutination assays and electron microscopy.
25852230	7	13	theme	critical	1100:1107	arg1	factor					1119:1124	a critical virulence factor	1098:1124	a critical virulence factor	1098:1124	Despite the difference in the CPS composition of different serotypes, this study has demonstrated for the first time that the CPS of a serotype other than 2 is also an important antiphagocytic factor and a critical virulence factor.
25852230	3	14	theme	capsular	347:354	arg1	CPS					372:374	CPS	372:374	CPS	372:374	To study the role of the capsular polysaccharide (CPS) of serotype 14 as a virulence factor, we constructed knockout mutants devoid of either cps14B, a highly conserved regulatory gene, or neu14C, a gene coding for uridine diphospho-N-acetylglucosamine 2-epimerase, which is involved in sialic acid synthesis.
25852230	3	14	theme	capsular	347:354	arg1	polysaccharide					356:369	the capsular polysaccharide	343:369	the capsular polysaccharide (CPS) of serotype 14	343:390	To study the role of the capsular polysaccharide (CPS) of serotype 14 as a virulence factor, we constructed knockout mutants devoid of either cps14B, a highly conserved regulatory gene, or neu14C, a gene coding for uridine diphospho-N-acetylglucosamine 2-epimerase, which is involved in sialic acid synthesis.
25852230	6	15	theme	in	795:796	arg1	model					810:814	An in vivo murine model	792:814	An in vivo murine model	792:814	An in vivo murine model was used to demonstrate attenuated virulence of this non-encapsulated mutant.
25852230	3	16	theme	serotype	380:387	arg1	CPS					372:374	CPS	372:374	CPS	372:374	To study the role of the capsular polysaccharide (CPS) of serotype 14 as a virulence factor, we constructed knockout mutants devoid of either cps14B, a highly conserved regulatory gene, or neu14C, a gene coding for uridine diphospho-N-acetylglucosamine 2-epimerase, which is involved in sialic acid synthesis.
25852230	3	16	theme	serotype	380:387	arg1	polysaccharide					356:369	the capsular polysaccharide	343:369	the capsular polysaccharide (CPS) of serotype 14	343:390	To study the role of the capsular polysaccharide (CPS) of serotype 14 as a virulence factor, we constructed knockout mutants devoid of either cps14B, a highly conserved regulatory gene, or neu14C, a gene coding for uridine diphospho-N-acetylglucosamine 2-epimerase, which is involved in sialic acid synthesis.
25852230	0	17	theme	capsular	12:19	arg1	polysaccharide					21:34	the capsular polysaccharide	8:34	the capsular polysaccharide	8:34	Role of the capsular polysaccharide as a virulence factor for Streptococcus suis serotype 14.
25852230	3	18	theme	regulatory	491:500	arg1	gene					502:505	a highly conserved regulatory gene	472:505	a highly conserved regulatory gene	472:505	To study the role of the capsular polysaccharide (CPS) of serotype 14 as a virulence factor, we constructed knockout mutants devoid of either cps14B, a highly conserved regulatory gene, or neu14C, a gene coding for uridine diphospho-N-acetylglucosamine 2-epimerase, which is involved in sialic acid synthesis.
25852230	3	18	theme	regulatory	491:500	arg1	cps14B					464:469	cps14B	464:469	cps14B	464:469	To study the role of the capsular polysaccharide (CPS) of serotype 14 as a virulence factor, we constructed knockout mutants devoid of either cps14B, a highly conserved regulatory gene, or neu14C, a gene coding for uridine diphospho-N-acetylglucosamine 2-epimerase, which is involved in sialic acid synthesis.
25852230	5	19	theme	Δcps14B	783:789	arg1	susceptibility					758:771	high susceptibility	753:771	high susceptibility of mutant Δcps14B	753:789	Phagocytosis assays showed high susceptibility of mutant Δcps14B.
25852230	5	20	theme	mutant	776:781	arg1	Δcps14B					783:789	mutant Δcps14B	776:789	mutant Δcps14B	776:789	Phagocytosis assays showed high susceptibility of mutant Δcps14B.
25852230	6	21	dep	in	795:796	arg1	vivo					798:801	vivo	798:801	vivo	798:801	An in vivo murine model was used to demonstrate attenuated virulence of this non-encapsulated mutant.
25852230	1	22	theme	Streptococcus	94:106	arg1	pathogen					135:142	an important swine pathogen	116:142	an important swine pathogen	116:142	Streptococcus suis is an important swine pathogen and a zoonotic agent causing meningitis and septicemia.
25852230	1	22	theme	Streptococcus	94:106	arg1	suis					108:111	Streptococcus suis	94:111	Streptococcus suis	94:111	Streptococcus suis is an important swine pathogen and a zoonotic agent causing meningitis and septicemia.
25852230	7	23	from	difference	906:915	arg1	composition					928:938	the CPS composition	920:938	the CPS composition of different serotypes	920:961	Despite the difference in the CPS composition of different serotypes, this study has demonstrated for the first time that the CPS of a serotype other than 2 is also an important antiphagocytic factor and a critical virulence factor.
25852230	7	24	theme	antiphagocytic	1072:1085	arg1	factor					1087:1092	an important antiphagocytic factor	1059:1092	an important antiphagocytic factor	1059:1092	Despite the difference in the CPS composition of different serotypes, this study has demonstrated for the first time that the CPS of a serotype other than 2 is also an important antiphagocytic factor and a critical virulence factor.
25852230	7	24	theme	antiphagocytic	1072:1085	arg1	CPS					1020:1022	the CPS	1016:1022	the CPS of a serotype other than 2	1016:1049	Despite the difference in the CPS composition of different serotypes, this study has demonstrated for the first time that the CPS of a serotype other than 2 is also an important antiphagocytic factor and a critical virulence factor.
25852230	7	25	theme	virulence	1109:1117	arg1	factor					1119:1124	a critical virulence factor	1098:1124	a critical virulence factor	1098:1124	Despite the difference in the CPS composition of different serotypes, this study has demonstrated for the first time that the CPS of a serotype other than 2 is also an important antiphagocytic factor and a critical virulence factor.
25852230	0	26	theme	polysaccharide	21:34	arg1	Role					0:3	Role	0:3	Role of the capsular polysaccharide as a virulence factor for Streptococcus suis serotype 14.	0:92	Role of the capsular polysaccharide as a virulence factor for Streptococcus suis serotype 14.
25852230	5	27	theme	Phagocytosis	726:737	arg1	assays					739:744	Phagocytosis assays	726:744	Phagocytosis assays	726:744	Phagocytosis assays showed high susceptibility of mutant Δcps14B.
25852230	3	28	theme	2-epimerase	575:585	arg1	diphospho-N-acetylglucosamine					545:573	uridine diphospho-N-acetylglucosamine 2-epimerase	537:585	uridine diphospho-N-acetylglucosamine 2-epimerase	537:585	To study the role of the capsular polysaccharide (CPS) of serotype 14 as a virulence factor, we constructed knockout mutants devoid of either cps14B, a highly conserved regulatory gene, or neu14C, a gene coding for uridine diphospho-N-acetylglucosamine 2-epimerase, which is involved in sialic acid synthesis.
25852230	3	29	theme	virulence	397:405	arg1	factor					407:412	a virulence factor	395:412	a virulence factor	395:412	To study the role of the capsular polysaccharide (CPS) of serotype 14 as a virulence factor, we constructed knockout mutants devoid of either cps14B, a highly conserved regulatory gene, or neu14C, a gene coding for uridine diphospho-N-acetylglucosamine 2-epimerase, which is involved in sialic acid synthesis.
25852230	3	30	theme	polysaccharide	356:369	arg1	role					335:338	the role	331:338	the role of the capsular polysaccharide (CPS) of serotype 14 as a virulence factor	331:412	To study the role of the capsular polysaccharide (CPS) of serotype 14 as a virulence factor, we constructed knockout mutants devoid of either cps14B, a highly conserved regulatory gene, or neu14C, a gene coding for uridine diphospho-N-acetylglucosamine 2-epimerase, which is involved in sialic acid synthesis.
25852230	3	31	theme	neu14C	511:516	arg1	devoid					447:452	devoid	447:452	devoid	447:452	To study the role of the capsular polysaccharide (CPS) of serotype 14 as a virulence factor, we constructed knockout mutants devoid of either cps14B, a highly conserved regulatory gene, or neu14C, a gene coding for uridine diphospho-N-acetylglucosamine 2-epimerase, which is involved in sialic acid synthesis.
25852230	3	32	theme	knockout	430:437	arg1	mutants					439:445	knockout mutants	430:445	knockout mutants devoid of either cps14B, a highly conserved regulatory gene, or neu14C, a gene coding for uridine diphospho-N-acetylglucosamine 2-epimerase, which is involved in sialic acid synthesis	430:629	To study the role of the capsular polysaccharide (CPS) of serotype 14 as a virulence factor, we constructed knockout mutants devoid of either cps14B, a highly conserved regulatory gene, or neu14C, a gene coding for uridine diphospho-N-acetylglucosamine 2-epimerase, which is involved in sialic acid synthesis.
25852230	7	33	theme	important	1062:1070	arg1	factor					1087:1092	an important antiphagocytic factor	1059:1092	an important antiphagocytic factor	1059:1092	Despite the difference in the CPS composition of different serotypes, this study has demonstrated for the first time that the CPS of a serotype other than 2 is also an important antiphagocytic factor and a critical virulence factor.
25852230	7	33	theme	important	1062:1070	arg1	CPS					1020:1022	the CPS	1016:1022	the CPS of a serotype other than 2	1016:1049	Despite the difference in the CPS composition of different serotypes, this study has demonstrated for the first time that the CPS of a serotype other than 2 is also an important antiphagocytic factor and a critical virulence factor.
25852230	7	34	theme	first	1000:1004	arg1	time					1006:1009	the first time that the CPS of a serotype other than 2 is also an important antiphagocytic factor and a critical virulence factor	996:1124	the first time that the CPS of a serotype other than 2 is also an important antiphagocytic factor and a critical virulence factor	996:1124	Despite the difference in the CPS composition of different serotypes, this study has demonstrated for the first time that the CPS of a serotype other than 2 is also an important antiphagocytic factor and a critical virulence factor.
25852230	3	35	theme	sialic	609:614	arg1	acid					616:619	sialic acid	609:619	sialic acid synthesis	609:629	To study the role of the capsular polysaccharide (CPS) of serotype 14 as a virulence factor, we constructed knockout mutants devoid of either cps14B, a highly conserved regulatory gene, or neu14C, a gene coding for uridine diphospho-N-acetylglucosamine 2-epimerase, which is involved in sialic acid synthesis.
25852230	6	36	theme	murine	803:808	arg1	model					810:814	An in vivo murine model	792:814	An in vivo murine model	792:814	An in vivo murine model was used to demonstrate attenuated virulence of this non-encapsulated mutant.
25852230	6	37	theme	mutant	886:891	arg1	virulence					851:859	attenuated virulence	840:859	attenuated virulence of this non-encapsulated mutant	840:891	An in vivo murine model was used to demonstrate attenuated virulence of this non-encapsulated mutant.
25852230	3	38	theme	acid	616:619	arg1	synthesis					621:629	sialic acid synthesis	609:629	sialic acid synthesis	609:629	To study the role of the capsular polysaccharide (CPS) of serotype 14 as a virulence factor, we constructed knockout mutants devoid of either cps14B, a highly conserved regulatory gene, or neu14C, a gene coding for uridine diphospho-N-acetylglucosamine 2-epimerase, which is involved in sialic acid synthesis.
25852230	3	39	theme	uridine	537:543	arg1	diphospho-N-acetylglucosamine					545:573	uridine diphospho-N-acetylglucosamine 2-epimerase	537:585	uridine diphospho-N-acetylglucosamine 2-epimerase	537:585	To study the role of the capsular polysaccharide (CPS) of serotype 14 as a virulence factor, we constructed knockout mutants devoid of either cps14B, a highly conserved regulatory gene, or neu14C, a gene coding for uridine diphospho-N-acetylglucosamine 2-epimerase, which is involved in sialic acid synthesis.
25852230	2	40	theme	pathogenesis	297:308	arg1	understanding					276:288	understanding	276:288	understanding of its pathogenesis	276:308	Although serotype 2 is the most virulent type, serotype 14 is emerging, and understanding of its pathogenesis is limited.
25852230	4	41	theme	coagglutination	678:692	arg1	assays					694:699	coagglutination assays	678:699	coagglutination assays	678:699	The mutants showed total loss of the CPS with coagglutination assays and electron microscopy.
25852230	7	42	theme	different	943:951	arg1	serotypes					953:961	different serotypes	943:961	different serotypes	943:961	Despite the difference in the CPS composition of different serotypes, this study has demonstrated for the first time that the CPS of a serotype other than 2 is also an important antiphagocytic factor and a critical virulence factor.
25852230	1	43	theme	important	119:127	arg1	pathogen					135:142	an important swine pathogen	116:142	an important swine pathogen	116:142	Streptococcus suis is an important swine pathogen and a zoonotic agent causing meningitis and septicemia.
25852230	1	43	theme	important	119:127	arg1	suis					108:111	Streptococcus suis	94:111	Streptococcus suis	94:111	Streptococcus suis is an important swine pathogen and a zoonotic agent causing meningitis and septicemia.
25852230	6	44	used	used	820:823	arg2	model					810:814	An in vivo murine model	792:814	An in vivo murine model	792:814	An in vivo murine model was used to demonstrate attenuated virulence of this non-encapsulated mutant.
25852230	2	45	theme	virulent	232:239	arg1	serotype					209:216	serotype 2	209:218	serotype 2	209:218	Although serotype 2 is the most virulent type, serotype 14 is emerging, and understanding of its pathogenesis is limited.
25852230	2	45	theme	virulent	232:239	arg1	type					241:244	the most virulent type	223:244	the most virulent type	223:244	Although serotype 2 is the most virulent type, serotype 14 is emerging, and understanding of its pathogenesis is limited.
25852230	7	46	theme	serotypes	953:961	arg1	composition					928:938	the CPS composition	920:938	the CPS composition of different serotypes	920:961	Despite the difference in the CPS composition of different serotypes, this study has demonstrated for the first time that the CPS of a serotype other than 2 is also an important antiphagocytic factor and a critical virulence factor.
25852230	1	47	theme	swine	129:133	arg1	pathogen					135:142	an important swine pathogen	116:142	an important swine pathogen	116:142	Streptococcus suis is an important swine pathogen and a zoonotic agent causing meningitis and septicemia.
25852230	1	47	theme	swine	129:133	arg1	suis					108:111	Streptococcus suis	94:111	Streptococcus suis	94:111	Streptococcus suis is an important swine pathogen and a zoonotic agent causing meningitis and septicemia.
25852230	0	48	theme	virulence	41:49	arg1	factor					51:56	a virulence factor	39:56	a virulence factor for Streptococcus suis serotype 14	39:91	Role of the capsular polysaccharide as a virulence factor for Streptococcus suis serotype 14.
25852230	6	49	theme	non-encapsulated	869:884	arg1	mutant					886:891	this non-encapsulated mutant	864:891	this non-encapsulated mutant	864:891	An in vivo murine model was used to demonstrate attenuated virulence of this non-encapsulated mutant.
25852230	3	50	theme	cps14B	464:469	arg1	devoid					447:452	devoid	447:452	devoid	447:452	To study the role of the capsular polysaccharide (CPS) of serotype 14 as a virulence factor, we constructed knockout mutants devoid of either cps14B, a highly conserved regulatory gene, or neu14C, a gene coding for uridine diphospho-N-acetylglucosamine 2-epimerase, which is involved in sialic acid synthesis.
24166004	7	0	theme	G	1552:1552	arg1	alginate					1554:1561	the High G alginate	1543:1561	the High G alginate	1543:1561	The addition of a PLL coating to the High G alginate prevented cell escape from beads for up to 14 days.
24166004	4	1	theme	varying	951:957	arg1	compositions					968:979	varying alginate compositions	951:979	varying alginate compositions	951:979	Therefore, the objective of this study was to systematically examine the effects of varying alginate compositions on microencapsulated ESC expansion and phenotype.
24166004	9	2	theme	stem	1989:1992	arg1	fate					1999:2002	stem cell fate	1989:2002	stem cell fate	1989:2002	Taken together, the findings of this study suggest that distinct ESC expansion capacities and differentiation trajectories emerge depending on the alginate composition employed, indicating that encapsulation material physical properties can be used to control stem cell fate.
24166004	8	3	theme	primitive	1699:1707	arg1	phenotype					1718:1726	a primitive endoderm phenotype	1697:1726	a primitive endoderm phenotype	1697:1726	Furthermore, encapsulation within High M alginate promoted differentiation toward a primitive endoderm phenotype.
24166004	3	4	theme	parameters	795:804	arg1	impact					762:767	the impact	758:767	the impact of alginate encapsulation parameters on stem cell phenotype	758:827	Despite initial studies examining alginate microbeads as a platform for stem cell expansion and directed differentiation, the impact of alginate encapsulation parameters on stem cell phenotype has not been thoroughly investigated.
24166004	1	5	theme	viable	279:284	arg1	populations					306:316	viable and homogenous cell populations	279:316	viable and homogenous cell populations of sufficient numbers	279:338	Pluripotent embryonic stem cells (ESCs) have tremendous potential as tools for regenerative medicine and drug discovery, yet the lack of processes to manufacture viable and homogenous cell populations of sufficient numbers limits the clinical translation of current and future cell therapies.
24166004	7	6	theme	coating	1532:1538	arg1	addition					1514:1521	The addition	1510:1521	The addition of a PLL coating to the High G alginate	1510:1561	The addition of a PLL coating to the High G alginate prevented cell escape from beads for up to 14 days.
24166004	3	7	theme	alginate	772:779	arg1	parameters					795:804	alginate encapsulation parameters	772:804	alginate encapsulation parameters	772:804	Despite initial studies examining alginate microbeads as a platform for stem cell expansion and directed differentiation, the impact of alginate encapsulation parameters on stem cell phenotype has not been thoroughly investigated.
24166004	5	8	theme	PLL	1251:1253	arg1	coating					1256:1262	a poly-L-lysine (PLL) coating	1234:1262	a poly-L-lysine (PLL) coating	1234:1262	Pre-formed aggregates of murine ESCs were encapsulated in alginate microbeads composed of a high or low ratio of guluronic to mannuronic acid residues (High G and High M, respectively), with and without a poly-L-lysine (PLL) coating, thereby providing four distinct alginate bead compositions for analysis.
24166004	9	9	theme	distinct	1785:1792	arg1	capacities					1808:1817	distinct ESC expansion capacities	1785:1817	distinct ESC expansion capacities	1785:1817	Taken together, the findings of this study suggest that distinct ESC expansion capacities and differentiation trajectories emerge depending on the alginate composition employed, indicating that encapsulation material physical properties can be used to control stem cell fate.
24166004	1	10	theme	Pluripotent	117:127	arg1	ESCs					151:154	ESCs	151:154	ESCs	151:154	Pluripotent embryonic stem cells (ESCs) have tremendous potential as tools for regenerative medicine and drug discovery, yet the lack of processes to manufacture viable and homogenous cell populations of sufficient numbers limits the clinical translation of current and future cell therapies.
24166004	1	10	theme	Pluripotent	117:127	arg1	cells					144:148	Pluripotent embryonic stem cells	117:148	Pluripotent embryonic stem cells (ESCs)	117:155	Pluripotent embryonic stem cells (ESCs) have tremendous potential as tools for regenerative medicine and drug discovery, yet the lack of processes to manufacture viable and homogenous cell populations of sufficient numbers limits the clinical translation of current and future cell therapies.
24166004	3	11	theme	stem	809:812	arg1	cell					814:817	stem cell	809:817	stem cell phenotype	809:827	Despite initial studies examining alginate microbeads as a platform for stem cell expansion and directed differentiation, the impact of alginate encapsulation parameters on stem cell phenotype has not been thoroughly investigated.
24166004	1	12	theme	therapies	399:407	arg1	translation					360:370	the clinical translation	347:370	the clinical translation of current and future cell therapies	347:407	Pluripotent embryonic stem cells (ESCs) have tremendous potential as tools for regenerative medicine and drug discovery, yet the lack of processes to manufacture viable and homogenous cell populations of sufficient numbers limits the clinical translation of current and future cell therapies.
24166004	1	13	theme	drug	222:225	arg1	discovery					227:235	drug discovery	222:235	drug discovery	222:235	Pluripotent embryonic stem cells (ESCs) have tremendous potential as tools for regenerative medicine and drug discovery, yet the lack of processes to manufacture viable and homogenous cell populations of sufficient numbers limits the clinical translation of current and future cell therapies.
24166004	9	14	theme	study	1766:1770	arg1	findings					1749:1756	the findings	1745:1756	the findings of this study	1745:1770	Taken together, the findings of this study suggest that distinct ESC expansion capacities and differentiation trajectories emerge depending on the alginate composition employed, indicating that encapsulation material physical properties can be used to control stem cell fate.
24166004	5	15	theme	acid	1168:1171	arg1	G					1188:1188	High G	1183:1188	High G	1183:1188	Pre-formed aggregates of murine ESCs were encapsulated in alginate microbeads composed of a high or low ratio of guluronic to mannuronic acid residues (High G and High M, respectively), with and without a poly-L-lysine (PLL) coating, thereby providing four distinct alginate bead compositions for analysis.
24166004	5	15	theme	acid	1168:1171	arg1	M					1199:1199	High M	1194:1199	High M	1194:1199	Pre-formed aggregates of murine ESCs were encapsulated in alginate microbeads composed of a high or low ratio of guluronic to mannuronic acid residues (High G and High M, respectively), with and without a poly-L-lysine (PLL) coating, thereby providing four distinct alginate bead compositions for analysis.
24166004	5	15	theme	acid	1168:1171	arg1	residues					1173:1180	guluronic to mannuronic acid residues	1144:1180	guluronic to mannuronic acid residues (High G and High M, respectively)	1144:1214	Pre-formed aggregates of murine ESCs were encapsulated in alginate microbeads composed of a high or low ratio of guluronic to mannuronic acid residues (High G and High M, respectively), with and without a poly-L-lysine (PLL) coating, thereby providing four distinct alginate bead compositions for analysis.
24166004	4	16	theme	compositions	968:979	arg1	effects					940:946	the effects	936:946	the effects of varying alginate compositions on microencapsulated ESC expansion and phenotype	936:1028	Therefore, the objective of this study was to systematically examine the effects of varying alginate compositions on microencapsulated ESC expansion and phenotype.
24166004	2	17	theme	encapsulation	612:624	arg1	material					626:633	the encapsulation material	608:633	the encapsulation material	608:633	Microencapsulation of ESCs within microbeads can shield cells from hydrodynamic shear forces found in bioreactor environments while allowing for sufficient diffusion of nutrients and oxygen through the encapsulation material.
24166004	5	18	theme	low	1131:1133	arg1	ratio					1135:1139	a high or low ratio	1121:1139	a high or low ratio of guluronic to mannuronic acid residues (High G and High M, respectively), with and without a poly-L-lysine (PLL) coating, thereby providing four distinct alginate bead compositions for analysis	1121:1335	Pre-formed aggregates of murine ESCs were encapsulated in alginate microbeads composed of a high or low ratio of guluronic to mannuronic acid residues (High G and High M, respectively), with and without a poly-L-lysine (PLL) coating, thereby providing four distinct alginate bead compositions for analysis.
24166004	3	19	theme	initial	644:650	arg1	studies					652:658	initial studies	644:658	initial studies examining alginate microbeads as a platform for stem cell expansion and directed differentiation	644:755	Despite initial studies examining alginate microbeads as a platform for stem cell expansion and directed differentiation, the impact of alginate encapsulation parameters on stem cell phenotype has not been thoroughly investigated.
24166004	4	20	theme	microencapsulated	984:1000	arg1	expansion					1006:1014	expansion	1006:1014	expansion	1006:1014	Therefore, the objective of this study was to systematically examine the effects of varying alginate compositions on microencapsulated ESC expansion and phenotype.
24166004	4	20	theme	microencapsulated	984:1000	arg1	ESC					1002:1004	microencapsulated ESC expansion and phenotype	984:1028	microencapsulated ESC expansion and phenotype	984:1028	Therefore, the objective of this study was to systematically examine the effects of varying alginate compositions on microencapsulated ESC expansion and phenotype.
24166004	4	20	theme	microencapsulated	984:1000	arg1	phenotype					1020:1028	phenotype	1020:1028	phenotype	1020:1028	Therefore, the objective of this study was to systematically examine the effects of varying alginate compositions on microencapsulated ESC expansion and phenotype.
24166004	0	21	theme	embryonic	85:93	arg1	aggregates					105:114	microencapsulated embryonic stem cell aggregates	67:114	microencapsulated embryonic stem cell aggregates	67:114	Alginate encapsulation parameters influence the differentiation of microencapsulated embryonic stem cell aggregates.
24166004	5	22	theme	High	1194:1197	arg1	M					1199:1199	High M	1194:1199	High M	1194:1199	Pre-formed aggregates of murine ESCs were encapsulated in alginate microbeads composed of a high or low ratio of guluronic to mannuronic acid residues (High G and High M, respectively), with and without a poly-L-lysine (PLL) coating, thereby providing four distinct alginate bead compositions for analysis.
24166004	5	22	theme	High	1194:1197	arg1	residues					1173:1180	guluronic to mannuronic acid residues	1144:1180	guluronic to mannuronic acid residues (High G and High M, respectively)	1144:1214	Pre-formed aggregates of murine ESCs were encapsulated in alginate microbeads composed of a high or low ratio of guluronic to mannuronic acid residues (High G and High M, respectively), with and without a poly-L-lysine (PLL) coating, thereby providing four distinct alginate bead compositions for analysis.
24166004	1	23	theme	tremendous	162:171	arg1	tools					186:190	tools	186:190	tools for regenerative medicine and drug discovery	186:235	Pluripotent embryonic stem cells (ESCs) have tremendous potential as tools for regenerative medicine and drug discovery, yet the lack of processes to manufacture viable and homogenous cell populations of sufficient numbers limits the clinical translation of current and future cell therapies.
24166004	1	23	theme	tremendous	162:171	arg1	potential					173:181	tremendous potential	162:181	tremendous potential	162:181	Pluripotent embryonic stem cells (ESCs) have tremendous potential as tools for regenerative medicine and drug discovery, yet the lack of processes to manufacture viable and homogenous cell populations of sufficient numbers limits the clinical translation of current and future cell therapies.
24166004	9	24	theme	differentiation	1823:1837	arg1	trajectories					1839:1850	differentiation trajectories	1823:1850	differentiation trajectories	1823:1850	Taken together, the findings of this study suggest that distinct ESC expansion capacities and differentiation trajectories emerge depending on the alginate composition employed, indicating that encapsulation material physical properties can be used to control stem cell fate.
24166004	1	25	theme	cell	301:304	arg1	populations					306:316	viable and homogenous cell populations	279:316	viable and homogenous cell populations of sufficient numbers	279:338	Pluripotent embryonic stem cells (ESCs) have tremendous potential as tools for regenerative medicine and drug discovery, yet the lack of processes to manufacture viable and homogenous cell populations of sufficient numbers limits the clinical translation of current and future cell therapies.
24166004	5	26	theme	bead	1306:1309	arg1	compositions					1311:1322	four distinct alginate bead compositions	1283:1322	four distinct alginate bead compositions	1283:1322	Pre-formed aggregates of murine ESCs were encapsulated in alginate microbeads composed of a high or low ratio of guluronic to mannuronic acid residues (High G and High M, respectively), with and without a poly-L-lysine (PLL) coating, thereby providing four distinct alginate bead compositions for analysis.
24166004	0	27	theme	cell	100:103	arg1	aggregates					105:114	microencapsulated embryonic stem cell aggregates	67:114	microencapsulated embryonic stem cell aggregates	67:114	Alginate encapsulation parameters influence the differentiation of microencapsulated embryonic stem cell aggregates.
24166004	4	28	from	effects	940:946	arg1	expansion					1006:1014	expansion	1006:1014	expansion	1006:1014	Therefore, the objective of this study was to systematically examine the effects of varying alginate compositions on microencapsulated ESC expansion and phenotype.
24166004	4	28	from	effects	940:946	arg1	ESC					1002:1004	microencapsulated ESC expansion and phenotype	984:1028	microencapsulated ESC expansion and phenotype	984:1028	Therefore, the objective of this study was to systematically examine the effects of varying alginate compositions on microencapsulated ESC expansion and phenotype.
24166004	4	28	from	effects	940:946	arg1	phenotype					1020:1028	phenotype	1020:1028	phenotype	1020:1028	Therefore, the objective of this study was to systematically examine the effects of varying alginate compositions on microencapsulated ESC expansion and phenotype.
24166004	1	29	theme	current	375:381	arg1	therapies					399:407	current and future cell therapies	375:407	current and future cell therapies	375:407	Pluripotent embryonic stem cells (ESCs) have tremendous potential as tools for regenerative medicine and drug discovery, yet the lack of processes to manufacture viable and homogenous cell populations of sufficient numbers limits the clinical translation of current and future cell therapies.
24166004	5	30	theme	ESCs	1063:1066	arg1	aggregates					1042:1051	Pre-formed aggregates	1031:1051	Pre-formed aggregates of murine ESCs	1031:1066	Pre-formed aggregates of murine ESCs were encapsulated in alginate microbeads composed of a high or low ratio of guluronic to mannuronic acid residues (High G and High M, respectively), with and without a poly-L-lysine (PLL) coating, thereby providing four distinct alginate bead compositions for analysis.
24166004	2	31	theme	sufficient	555:564	arg1	diffusion					566:574	sufficient diffusion	555:574	sufficient diffusion of nutrients and oxygen through the encapsulation material	555:633	Microencapsulation of ESCs within microbeads can shield cells from hydrodynamic shear forces found in bioreactor environments while allowing for sufficient diffusion of nutrients and oxygen through the encapsulation material.
24166004	4	32	dep	ESC	1002:1004	arg1	expansion					1006:1014	expansion	1006:1014	expansion	1006:1014	Therefore, the objective of this study was to systematically examine the effects of varying alginate compositions on microencapsulated ESC expansion and phenotype.
24166004	4	32	dep	ESC	1002:1004	arg1	ESC					1002:1004	microencapsulated ESC expansion and phenotype	984:1028	microencapsulated ESC expansion and phenotype	984:1028	Therefore, the objective of this study was to systematically examine the effects of varying alginate compositions on microencapsulated ESC expansion and phenotype.
24166004	4	32	dep	ESC	1002:1004	arg1	phenotype					1020:1028	phenotype	1020:1028	phenotype	1020:1028	Therefore, the objective of this study was to systematically examine the effects of varying alginate compositions on microencapsulated ESC expansion and phenotype.
24166004	1	33	theme	future	387:392	arg1	therapies					399:407	current and future cell therapies	375:407	current and future cell therapies	375:407	Pluripotent embryonic stem cells (ESCs) have tremendous potential as tools for regenerative medicine and drug discovery, yet the lack of processes to manufacture viable and homogenous cell populations of sufficient numbers limits the clinical translation of current and future cell therapies.
24166004	2	34	theme	hydrodynamic	477:488	arg1	forces					496:501	hydrodynamic shear forces	477:501	hydrodynamic shear forces found in bioreactor environments	477:534	Microencapsulation of ESCs within microbeads can shield cells from hydrodynamic shear forces found in bioreactor environments while allowing for sufficient diffusion of nutrients and oxygen through the encapsulation material.
24166004	0	35	theme	Alginate	0:7	arg1	parameters					23:32	Alginate encapsulation parameters	0:32	Alginate encapsulation parameters	0:32	Alginate encapsulation parameters influence the differentiation of microencapsulated embryonic stem cell aggregates.
24166004	5	36	theme	Pre-formed	1031:1040	arg1	aggregates					1042:1051	Pre-formed aggregates	1031:1051	Pre-formed aggregates of murine ESCs	1031:1066	Pre-formed aggregates of murine ESCs were encapsulated in alginate microbeads composed of a high or low ratio of guluronic to mannuronic acid residues (High G and High M, respectively), with and without a poly-L-lysine (PLL) coating, thereby providing four distinct alginate bead compositions for analysis.
24166004	6	37	theme	High	1443:1446	arg1	alginate					1450:1457	High G alginate	1443:1457	High G alginate yielding the least differentiated cell population	1443:1507	Encapsulation in all alginate compositions was found to delay differentiation, with encapsulation within High G alginate yielding the least differentiated cell population.
24166004	5	38	theme	poly-L-lysine	1236:1248	arg1	coating					1256:1262	a poly-L-lysine (PLL) coating	1234:1262	a poly-L-lysine (PLL) coating	1234:1262	Pre-formed aggregates of murine ESCs were encapsulated in alginate microbeads composed of a high or low ratio of guluronic to mannuronic acid residues (High G and High M, respectively), with and without a poly-L-lysine (PLL) coating, thereby providing four distinct alginate bead compositions for analysis.
24166004	1	39	theme	stem	139:142	arg1	ESCs					151:154	ESCs	151:154	ESCs	151:154	Pluripotent embryonic stem cells (ESCs) have tremendous potential as tools for regenerative medicine and drug discovery, yet the lack of processes to manufacture viable and homogenous cell populations of sufficient numbers limits the clinical translation of current and future cell therapies.
24166004	1	39	theme	stem	139:142	arg1	cells					144:148	Pluripotent embryonic stem cells	117:148	Pluripotent embryonic stem cells (ESCs)	117:155	Pluripotent embryonic stem cells (ESCs) have tremendous potential as tools for regenerative medicine and drug discovery, yet the lack of processes to manufacture viable and homogenous cell populations of sufficient numbers limits the clinical translation of current and future cell therapies.
24166004	9	40	theme	ESC	1794:1796	arg1	capacities					1808:1817	distinct ESC expansion capacities	1785:1817	distinct ESC expansion capacities	1785:1817	Taken together, the findings of this study suggest that distinct ESC expansion capacities and differentiation trajectories emerge depending on the alginate composition employed, indicating that encapsulation material physical properties can be used to control stem cell fate.
24166004	3	41	theme	cell	713:716	arg1	expansion					718:726	stem cell expansion	708:726	stem cell expansion	708:726	Despite initial studies examining alginate microbeads as a platform for stem cell expansion and directed differentiation, the impact of alginate encapsulation parameters on stem cell phenotype has not been thoroughly investigated.
24166004	6	42	theme	differentiated	1478:1491	arg1	population					1498:1507	the least differentiated cell population	1468:1507	the least differentiated cell population	1468:1507	Encapsulation in all alginate compositions was found to delay differentiation, with encapsulation within High G alginate yielding the least differentiated cell population.
24166004	9	43	theme	encapsulation	1923:1935	arg1	properties					1955:1964	encapsulation material physical properties	1923:1964	encapsulation material physical properties	1923:1964	Taken together, the findings of this study suggest that distinct ESC expansion capacities and differentiation trajectories emerge depending on the alginate composition employed, indicating that encapsulation material physical properties can be used to control stem cell fate.
24166004	4	44	theme	study	900:904	arg1	objective					882:890	the objective	878:890	the objective of this study	878:904	Therefore, the objective of this study was to systematically examine the effects of varying alginate compositions on microencapsulated ESC expansion and phenotype.
24166004	1	45	theme	numbers	332:338	arg1	populations					306:316	viable and homogenous cell populations	279:316	viable and homogenous cell populations of sufficient numbers	279:338	Pluripotent embryonic stem cells (ESCs) have tremendous potential as tools for regenerative medicine and drug discovery, yet the lack of processes to manufacture viable and homogenous cell populations of sufficient numbers limits the clinical translation of current and future cell therapies.
24166004	7	46	theme	cell	1573:1576	arg1	escape					1578:1583	cell escape	1573:1583	cell escape from beads for up to 14 days	1573:1612	The addition of a PLL coating to the High G alginate prevented cell escape from beads for up to 14 days.
24166004	9	47	theme	physical	1946:1953	arg1	properties					1955:1964	encapsulation material physical properties	1923:1964	encapsulation material physical properties	1923:1964	Taken together, the findings of this study suggest that distinct ESC expansion capacities and differentiation trajectories emerge depending on the alginate composition employed, indicating that encapsulation material physical properties can be used to control stem cell fate.
24166004	8	48	theme	endoderm	1709:1716	arg1	phenotype					1718:1726	a primitive endoderm phenotype	1697:1726	a primitive endoderm phenotype	1697:1726	Furthermore, encapsulation within High M alginate promoted differentiation toward a primitive endoderm phenotype.
24166004	5	49	theme	alginate	1089:1096	arg1	microbeads					1098:1107	alginate microbeads	1089:1107	alginate microbeads composed of a high or low ratio of guluronic to mannuronic acid residues (High G and High M, respectively), with and without a poly-L-lysine (PLL) coating, thereby providing four distinct alginate bead compositions for analysis	1089:1335	Pre-formed aggregates of murine ESCs were encapsulated in alginate microbeads composed of a high or low ratio of guluronic to mannuronic acid residues (High G and High M, respectively), with and without a poly-L-lysine (PLL) coating, thereby providing four distinct alginate bead compositions for analysis.
24166004	9	50	used	used	1973:1976	arg2	properties					1955:1964	encapsulation material physical properties	1923:1964	encapsulation material physical properties	1923:1964	Taken together, the findings of this study suggest that distinct ESC expansion capacities and differentiation trajectories emerge depending on the alginate composition employed, indicating that encapsulation material physical properties can be used to control stem cell fate.
24166004	5	51	dep	residues	1173:1180	arg1	G					1188:1188	High G	1183:1188	High G	1183:1188	Pre-formed aggregates of murine ESCs were encapsulated in alginate microbeads composed of a high or low ratio of guluronic to mannuronic acid residues (High G and High M, respectively), with and without a poly-L-lysine (PLL) coating, thereby providing four distinct alginate bead compositions for analysis.
24166004	5	51	dep	residues	1173:1180	arg1	M					1199:1199	High M	1194:1199	High M	1194:1199	Pre-formed aggregates of murine ESCs were encapsulated in alginate microbeads composed of a high or low ratio of guluronic to mannuronic acid residues (High G and High M, respectively), with and without a poly-L-lysine (PLL) coating, thereby providing four distinct alginate bead compositions for analysis.
24166004	5	51	dep	residues	1173:1180	arg1	residues					1173:1180	guluronic to mannuronic acid residues	1144:1180	guluronic to mannuronic acid residues (High G and High M, respectively)	1144:1214	Pre-formed aggregates of murine ESCs were encapsulated in alginate microbeads composed of a high or low ratio of guluronic to mannuronic acid residues (High G and High M, respectively), with and without a poly-L-lysine (PLL) coating, thereby providing four distinct alginate bead compositions for analysis.
24166004	5	51	dep	residues	1173:1180	arg1	providing					1273:1281	providing	1273:1281	providing four distinct alginate bead compositions for analysis	1273:1335	Pre-formed aggregates of murine ESCs were encapsulated in alginate microbeads composed of a high or low ratio of guluronic to mannuronic acid residues (High G and High M, respectively), with and without a poly-L-lysine (PLL) coating, thereby providing four distinct alginate bead compositions for analysis.
24166004	0	52	theme	microencapsulated	67:83	arg1	aggregates					105:114	microencapsulated embryonic stem cell aggregates	67:114	microencapsulated embryonic stem cell aggregates	67:114	Alginate encapsulation parameters influence the differentiation of microencapsulated embryonic stem cell aggregates.
24166004	7	53	theme	High	1547:1550	arg1	alginate					1554:1561	the High G alginate	1543:1561	the High G alginate	1543:1561	The addition of a PLL coating to the High G alginate prevented cell escape from beads for up to 14 days.
24166004	5	54	dep	mannuronic	1157:1166	arg1	to					1154:1155	to	1154:1155	to	1154:1155	Pre-formed aggregates of murine ESCs were encapsulated in alginate microbeads composed of a high or low ratio of guluronic to mannuronic acid residues (High G and High M, respectively), with and without a poly-L-lysine (PLL) coating, thereby providing four distinct alginate bead compositions for analysis.
24166004	6	55	theme	G	1448:1448	arg1	alginate					1450:1457	High G alginate	1443:1457	High G alginate yielding the least differentiated cell population	1443:1507	Encapsulation in all alginate compositions was found to delay differentiation, with encapsulation within High G alginate yielding the least differentiated cell population.
24166004	3	56	theme	encapsulation	781:793	arg1	parameters					795:804	alginate encapsulation parameters	772:804	alginate encapsulation parameters	772:804	Despite initial studies examining alginate microbeads as a platform for stem cell expansion and directed differentiation, the impact of alginate encapsulation parameters on stem cell phenotype has not been thoroughly investigated.
24166004	1	57	theme	processes	254:262	arg1	lack					246:249	the lack	242:249	the lack of processes to manufacture viable and homogenous cell populations of sufficient numbers	242:338	Pluripotent embryonic stem cells (ESCs) have tremendous potential as tools for regenerative medicine and drug discovery, yet the lack of processes to manufacture viable and homogenous cell populations of sufficient numbers limits the clinical translation of current and future cell therapies.
24166004	2	58	located	found	503:507	arg2	forces					496:501	hydrodynamic shear forces	477:501	hydrodynamic shear forces found in bioreactor environments	477:534	Microencapsulation of ESCs within microbeads can shield cells from hydrodynamic shear forces found in bioreactor environments while allowing for sufficient diffusion of nutrients and oxygen through the encapsulation material.
24166004	2	58	located	found	503:507	arg1	environments					523:534	bioreactor environments	512:534	bioreactor environments	512:534	Microencapsulation of ESCs within microbeads can shield cells from hydrodynamic shear forces found in bioreactor environments while allowing for sufficient diffusion of nutrients and oxygen through the encapsulation material.
24166004	7	59	from	beads	1590:1594	arg1	escape					1578:1583	cell escape	1573:1583	cell escape from beads for up to 14 days	1573:1612	The addition of a PLL coating to the High G alginate prevented cell escape from beads for up to 14 days.
24166004	7	60	theme	PLL	1528:1530	arg1	coating					1532:1538	a PLL coating	1526:1538	a PLL coating	1526:1538	The addition of a PLL coating to the High G alginate prevented cell escape from beads for up to 14 days.
24166004	9	61	theme	cell	1994:1997	arg1	fate					1999:2002	stem cell fate	1989:2002	stem cell fate	1989:2002	Taken together, the findings of this study suggest that distinct ESC expansion capacities and differentiation trajectories emerge depending on the alginate composition employed, indicating that encapsulation material physical properties can be used to control stem cell fate.
24166004	9	62	theme	material	1937:1944	arg1	properties					1955:1964	encapsulation material physical properties	1923:1964	encapsulation material physical properties	1923:1964	Taken together, the findings of this study suggest that distinct ESC expansion capacities and differentiation trajectories emerge depending on the alginate composition employed, indicating that encapsulation material physical properties can be used to control stem cell fate.
24166004	1	63	contain	have	157:160	arg1	ESCs					151:154	ESCs	151:154	ESCs	151:154	Pluripotent embryonic stem cells (ESCs) have tremendous potential as tools for regenerative medicine and drug discovery, yet the lack of processes to manufacture viable and homogenous cell populations of sufficient numbers limits the clinical translation of current and future cell therapies.
24166004	1	63	contain	have	157:160	arg2	tools					186:190	tools	186:190	tools for regenerative medicine and drug discovery	186:235	Pluripotent embryonic stem cells (ESCs) have tremendous potential as tools for regenerative medicine and drug discovery, yet the lack of processes to manufacture viable and homogenous cell populations of sufficient numbers limits the clinical translation of current and future cell therapies.
24166004	1	63	contain	have	157:160	arg1	cells					144:148	Pluripotent embryonic stem cells	117:148	Pluripotent embryonic stem cells (ESCs)	117:155	Pluripotent embryonic stem cells (ESCs) have tremendous potential as tools for regenerative medicine and drug discovery, yet the lack of processes to manufacture viable and homogenous cell populations of sufficient numbers limits the clinical translation of current and future cell therapies.
24166004	1	63	contain	have	157:160	arg2	potential					173:181	tremendous potential	162:181	tremendous potential	162:181	Pluripotent embryonic stem cells (ESCs) have tremendous potential as tools for regenerative medicine and drug discovery, yet the lack of processes to manufacture viable and homogenous cell populations of sufficient numbers limits the clinical translation of current and future cell therapies.
24166004	1	64	theme	regenerative	196:207	arg1	medicine					209:216	regenerative medicine	196:216	regenerative medicine	196:216	Pluripotent embryonic stem cells (ESCs) have tremendous potential as tools for regenerative medicine and drug discovery, yet the lack of processes to manufacture viable and homogenous cell populations of sufficient numbers limits the clinical translation of current and future cell therapies.
24166004	2	65	theme	oxygen	593:598	arg1	diffusion					566:574	sufficient diffusion	555:574	sufficient diffusion of nutrients and oxygen through the encapsulation material	555:633	Microencapsulation of ESCs within microbeads can shield cells from hydrodynamic shear forces found in bioreactor environments while allowing for sufficient diffusion of nutrients and oxygen through the encapsulation material.
24166004	3	66	theme	cell	814:817	arg1	phenotype					819:827	stem cell phenotype	809:827	stem cell phenotype	809:827	Despite initial studies examining alginate microbeads as a platform for stem cell expansion and directed differentiation, the impact of alginate encapsulation parameters on stem cell phenotype has not been thoroughly investigated.
24166004	5	67	theme	mannuronic	1157:1166	arg1	G					1188:1188	High G	1183:1188	High G	1183:1188	Pre-formed aggregates of murine ESCs were encapsulated in alginate microbeads composed of a high or low ratio of guluronic to mannuronic acid residues (High G and High M, respectively), with and without a poly-L-lysine (PLL) coating, thereby providing four distinct alginate bead compositions for analysis.
24166004	5	67	theme	mannuronic	1157:1166	arg1	M					1199:1199	High M	1194:1199	High M	1194:1199	Pre-formed aggregates of murine ESCs were encapsulated in alginate microbeads composed of a high or low ratio of guluronic to mannuronic acid residues (High G and High M, respectively), with and without a poly-L-lysine (PLL) coating, thereby providing four distinct alginate bead compositions for analysis.
24166004	5	67	theme	mannuronic	1157:1166	arg1	residues					1173:1180	guluronic to mannuronic acid residues	1144:1180	guluronic to mannuronic acid residues (High G and High M, respectively)	1144:1214	Pre-formed aggregates of murine ESCs were encapsulated in alginate microbeads composed of a high or low ratio of guluronic to mannuronic acid residues (High G and High M, respectively), with and without a poly-L-lysine (PLL) coating, thereby providing four distinct alginate bead compositions for analysis.
24166004	2	68	theme	nutrients	579:587	arg1	diffusion					566:574	sufficient diffusion	555:574	sufficient diffusion of nutrients and oxygen through the encapsulation material	555:633	Microencapsulation of ESCs within microbeads can shield cells from hydrodynamic shear forces found in bioreactor environments while allowing for sufficient diffusion of nutrients and oxygen through the encapsulation material.
24166004	3	69	from	impact	762:767	arg1	phenotype					819:827	stem cell phenotype	809:827	stem cell phenotype	809:827	Despite initial studies examining alginate microbeads as a platform for stem cell expansion and directed differentiation, the impact of alginate encapsulation parameters on stem cell phenotype has not been thoroughly investigated.
24166004	5	70	theme	residues	1173:1180	arg1	ratio					1135:1139	a high or low ratio	1121:1139	a high or low ratio of guluronic to mannuronic acid residues (High G and High M, respectively), with and without a poly-L-lysine (PLL) coating, thereby providing four distinct alginate bead compositions for analysis	1121:1335	Pre-formed aggregates of murine ESCs were encapsulated in alginate microbeads composed of a high or low ratio of guluronic to mannuronic acid residues (High G and High M, respectively), with and without a poly-L-lysine (PLL) coating, thereby providing four distinct alginate bead compositions for analysis.
24166004	4	71	theme	alginate	959:966	arg1	compositions					968:979	varying alginate compositions	951:979	varying alginate compositions	951:979	Therefore, the objective of this study was to systematically examine the effects of varying alginate compositions on microencapsulated ESC expansion and phenotype.
24166004	6	72	theme	alginate	1359:1366	arg1	compositions					1368:1379	all alginate compositions	1355:1379	all alginate compositions	1355:1379	Encapsulation in all alginate compositions was found to delay differentiation, with encapsulation within High G alginate yielding the least differentiated cell population.
24166004	7	73	dep	14	1606:1607	arg1	to					1603:1604	to	1603:1604	to	1603:1604	The addition of a PLL coating to the High G alginate prevented cell escape from beads for up to 14 days.
24166004	9	74	theme	expansion	1798:1806	arg1	capacities					1808:1817	distinct ESC expansion capacities	1785:1817	distinct ESC expansion capacities	1785:1817	Taken together, the findings of this study suggest that distinct ESC expansion capacities and differentiation trajectories emerge depending on the alginate composition employed, indicating that encapsulation material physical properties can be used to control stem cell fate.
24166004	0	75	theme	stem	95:98	arg1	aggregates					105:114	microencapsulated embryonic stem cell aggregates	67:114	microencapsulated embryonic stem cell aggregates	67:114	Alginate encapsulation parameters influence the differentiation of microencapsulated embryonic stem cell aggregates.
24166004	3	76	theme	alginate	670:677	arg1	microbeads					679:688	alginate microbeads	670:688	alginate microbeads	670:688	Despite initial studies examining alginate microbeads as a platform for stem cell expansion and directed differentiation, the impact of alginate encapsulation parameters on stem cell phenotype has not been thoroughly investigated.
24166004	3	76	theme	alginate	670:677	arg1	platform					695:702	a platform	693:702	a platform for stem cell expansion and directed differentiation	693:755	Despite initial studies examining alginate microbeads as a platform for stem cell expansion and directed differentiation, the impact of alginate encapsulation parameters on stem cell phenotype has not been thoroughly investigated.
24166004	5	77	theme	alginate	1297:1304	arg1	compositions					1311:1322	four distinct alginate bead compositions	1283:1322	four distinct alginate bead compositions	1283:1322	Pre-formed aggregates of murine ESCs were encapsulated in alginate microbeads composed of a high or low ratio of guluronic to mannuronic acid residues (High G and High M, respectively), with and without a poly-L-lysine (PLL) coating, thereby providing four distinct alginate bead compositions for analysis.
24166004	0	78	theme	aggregates	105:114	arg1	differentiation					48:62	the differentiation	44:62	the differentiation of microencapsulated embryonic stem cell aggregates	44:114	Alginate encapsulation parameters influence the differentiation of microencapsulated embryonic stem cell aggregates.
24166004	1	79	theme	clinical	351:358	arg1	translation					360:370	the clinical translation	347:370	the clinical translation of current and future cell therapies	347:407	Pluripotent embryonic stem cells (ESCs) have tremendous potential as tools for regenerative medicine and drug discovery, yet the lack of processes to manufacture viable and homogenous cell populations of sufficient numbers limits the clinical translation of current and future cell therapies.
24166004	2	80	theme	bioreactor	512:521	arg1	environments					523:534	bioreactor environments	512:534	bioreactor environments	512:534	Microencapsulation of ESCs within microbeads can shield cells from hydrodynamic shear forces found in bioreactor environments while allowing for sufficient diffusion of nutrients and oxygen through the encapsulation material.
24166004	5	81	theme	murine	1056:1061	arg1	ESCs					1063:1066	murine ESCs	1056:1066	murine ESCs	1056:1066	Pre-formed aggregates of murine ESCs were encapsulated in alginate microbeads composed of a high or low ratio of guluronic to mannuronic acid residues (High G and High M, respectively), with and without a poly-L-lysine (PLL) coating, thereby providing four distinct alginate bead compositions for analysis.
24166004	2	82	from	forces	496:501	arg1	cells					466:470	cells	466:470	cells from hydrodynamic shear forces found in bioreactor environments	466:534	Microencapsulation of ESCs within microbeads can shield cells from hydrodynamic shear forces found in bioreactor environments while allowing for sufficient diffusion of nutrients and oxygen through the encapsulation material.
24166004	5	83	theme	High	1183:1186	arg1	G					1188:1188	High G	1183:1188	High G	1183:1188	Pre-formed aggregates of murine ESCs were encapsulated in alginate microbeads composed of a high or low ratio of guluronic to mannuronic acid residues (High G and High M, respectively), with and without a poly-L-lysine (PLL) coating, thereby providing four distinct alginate bead compositions for analysis.
24166004	5	83	theme	High	1183:1186	arg1	residues					1173:1180	guluronic to mannuronic acid residues	1144:1180	guluronic to mannuronic acid residues (High G and High M, respectively)	1144:1214	Pre-formed aggregates of murine ESCs were encapsulated in alginate microbeads composed of a high or low ratio of guluronic to mannuronic acid residues (High G and High M, respectively), with and without a poly-L-lysine (PLL) coating, thereby providing four distinct alginate bead compositions for analysis.
24166004	8	84	theme	High	1649:1652	arg1	alginate					1656:1663	High M alginate	1649:1663	High M alginate	1649:1663	Furthermore, encapsulation within High M alginate promoted differentiation toward a primitive endoderm phenotype.
24166004	0	85	theme	encapsulation	9:21	arg1	parameters					23:32	Alginate encapsulation parameters	0:32	Alginate encapsulation parameters	0:32	Alginate encapsulation parameters influence the differentiation of microencapsulated embryonic stem cell aggregates.
24166004	1	86	theme	cell	394:397	arg1	therapies					399:407	current and future cell therapies	375:407	current and future cell therapies	375:407	Pluripotent embryonic stem cells (ESCs) have tremendous potential as tools for regenerative medicine and drug discovery, yet the lack of processes to manufacture viable and homogenous cell populations of sufficient numbers limits the clinical translation of current and future cell therapies.
24166004	1	87	theme	homogenous	290:299	arg1	populations					306:316	viable and homogenous cell populations	279:316	viable and homogenous cell populations of sufficient numbers	279:338	Pluripotent embryonic stem cells (ESCs) have tremendous potential as tools for regenerative medicine and drug discovery, yet the lack of processes to manufacture viable and homogenous cell populations of sufficient numbers limits the clinical translation of current and future cell therapies.
24166004	1	88	theme	embryonic	129:137	arg1	ESCs					151:154	ESCs	151:154	ESCs	151:154	Pluripotent embryonic stem cells (ESCs) have tremendous potential as tools for regenerative medicine and drug discovery, yet the lack of processes to manufacture viable and homogenous cell populations of sufficient numbers limits the clinical translation of current and future cell therapies.
24166004	1	88	theme	embryonic	129:137	arg1	cells					144:148	Pluripotent embryonic stem cells	117:148	Pluripotent embryonic stem cells (ESCs)	117:155	Pluripotent embryonic stem cells (ESCs) have tremendous potential as tools for regenerative medicine and drug discovery, yet the lack of processes to manufacture viable and homogenous cell populations of sufficient numbers limits the clinical translation of current and future cell therapies.
24166004	3	89	theme	stem	708:711	arg1	expansion					718:726	stem cell expansion	708:726	stem cell expansion	708:726	Despite initial studies examining alginate microbeads as a platform for stem cell expansion and directed differentiation, the impact of alginate encapsulation parameters on stem cell phenotype has not been thoroughly investigated.
24166004	6	90	theme	cell	1493:1496	arg1	population					1498:1507	the least differentiated cell population	1468:1507	the least differentiated cell population	1468:1507	Encapsulation in all alginate compositions was found to delay differentiation, with encapsulation within High G alginate yielding the least differentiated cell population.
24166004	2	91	theme	ESCs	432:435	arg1	Microencapsulation					410:427	Microencapsulation	410:427	Microencapsulation of ESCs within microbeads	410:453	Microencapsulation of ESCs within microbeads can shield cells from hydrodynamic shear forces found in bioreactor environments while allowing for sufficient diffusion of nutrients and oxygen through the encapsulation material.
24166004	6	92	theme	least	1472:1476	arg1	population					1498:1507	the least differentiated cell population	1468:1507	the least differentiated cell population	1468:1507	Encapsulation in all alginate compositions was found to delay differentiation, with encapsulation within High G alginate yielding the least differentiated cell population.
24166004	1	93	theme	sufficient	321:330	arg1	numbers					332:338	sufficient numbers	321:338	sufficient numbers	321:338	Pluripotent embryonic stem cells (ESCs) have tremendous potential as tools for regenerative medicine and drug discovery, yet the lack of processes to manufacture viable and homogenous cell populations of sufficient numbers limits the clinical translation of current and future cell therapies.
24166004	5	94	theme	high	1123:1126	arg1	ratio					1135:1139	a high or low ratio	1121:1139	a high or low ratio of guluronic to mannuronic acid residues (High G and High M, respectively), with and without a poly-L-lysine (PLL) coating, thereby providing four distinct alginate bead compositions for analysis	1121:1335	Pre-formed aggregates of murine ESCs were encapsulated in alginate microbeads composed of a high or low ratio of guluronic to mannuronic acid residues (High G and High M, respectively), with and without a poly-L-lysine (PLL) coating, thereby providing four distinct alginate bead compositions for analysis.
24166004	2	95	theme	shear	490:494	arg1	forces					496:501	hydrodynamic shear forces	477:501	hydrodynamic shear forces found in bioreactor environments	477:534	Microencapsulation of ESCs within microbeads can shield cells from hydrodynamic shear forces found in bioreactor environments while allowing for sufficient diffusion of nutrients and oxygen through the encapsulation material.
24166004	3	96	theme	directed	732:739	arg1	differentiation					741:755	directed differentiation	732:755	directed differentiation	732:755	Despite initial studies examining alginate microbeads as a platform for stem cell expansion and directed differentiation, the impact of alginate encapsulation parameters on stem cell phenotype has not been thoroughly investigated.
24166004	8	97	theme	M	1654:1654	arg1	alginate					1656:1663	High M alginate	1649:1663	High M alginate	1649:1663	Furthermore, encapsulation within High M alginate promoted differentiation toward a primitive endoderm phenotype.
24166004	5	98	theme	distinct	1288:1295	arg1	compositions					1311:1322	four distinct alginate bead compositions	1283:1322	four distinct alginate bead compositions	1283:1322	Pre-formed aggregates of murine ESCs were encapsulated in alginate microbeads composed of a high or low ratio of guluronic to mannuronic acid residues (High G and High M, respectively), with and without a poly-L-lysine (PLL) coating, thereby providing four distinct alginate bead compositions for analysis.
24166004	9	99	theme	alginate	1876:1883	arg1	composition					1885:1895	the alginate composition	1872:1895	the alginate composition employed	1872:1904	Taken together, the findings of this study suggest that distinct ESC expansion capacities and differentiation trajectories emerge depending on the alginate composition employed, indicating that encapsulation material physical properties can be used to control stem cell fate.
24166004	6	100	from	Encapsulation	1338:1350	arg1	compositions					1368:1379	all alginate compositions	1355:1379	all alginate compositions	1355:1379	Encapsulation in all alginate compositions was found to delay differentiation, with encapsulation within High G alginate yielding the least differentiated cell population.
25818153	1	0	theme	copper	313:318	arg1	CuAAC					353:357	CuAAC	353:357	CuAAC	353:357	BACKGROUND Poly(N-isopropylacrylamide) conjugated hyaluronan (HA-pN), a brush-like copolymer system which serves as a polymer vehicle for cellular and drug delivery, has been previously synthesized via the copper catalyzed azide-alkyne reaction (CuAAC) using a combination of copper sulfate and ascorbic acid (CuAsc) as the catalytic system of choice.
25818153	1	0	theme	copper	313:318	arg1	reaction					343:350	the copper catalyzed azide-alkyne reaction	309:350	the copper catalyzed azide-alkyne reaction (CuAAC) using a combination of copper sulfate and ascorbic acid (CuAsc) as the catalytic system of choice	309:456	BACKGROUND Poly(N-isopropylacrylamide) conjugated hyaluronan (HA-pN), a brush-like copolymer system which serves as a polymer vehicle for cellular and drug delivery, has been previously synthesized via the copper catalyzed azide-alkyne reaction (CuAAC) using a combination of copper sulfate and ascorbic acid (CuAsc) as the catalytic system of choice.
25818153	2	1	theme	aqueous	635:641	arg1	solvents					643:650	aqueous solvents	635:650	aqueous solvents	635:650	Bromotris(triphenylphosphine) copper(I) (CuBr(PPh3)3) is an alternative catalytic compound containing a phosphorous ligand which stabilizes copper in the +1 oxidative state in aqueous solvents and can be employed at true catalyst concentrations.
25818153	1	2	theme	catalyzed	320:328	arg1	CuAAC					353:357	CuAAC	353:357	CuAAC	353:357	BACKGROUND Poly(N-isopropylacrylamide) conjugated hyaluronan (HA-pN), a brush-like copolymer system which serves as a polymer vehicle for cellular and drug delivery, has been previously synthesized via the copper catalyzed azide-alkyne reaction (CuAAC) using a combination of copper sulfate and ascorbic acid (CuAsc) as the catalytic system of choice.
25818153	1	2	theme	catalyzed	320:328	arg1	reaction					343:350	the copper catalyzed azide-alkyne reaction	309:350	the copper catalyzed azide-alkyne reaction (CuAAC) using a combination of copper sulfate and ascorbic acid (CuAsc) as the catalytic system of choice	309:456	BACKGROUND Poly(N-isopropylacrylamide) conjugated hyaluronan (HA-pN), a brush-like copolymer system which serves as a polymer vehicle for cellular and drug delivery, has been previously synthesized via the copper catalyzed azide-alkyne reaction (CuAAC) using a combination of copper sulfate and ascorbic acid (CuAsc) as the catalytic system of choice.
25818153	1	3	theme	acid	411:414	arg1	combination					368:378	a combination	366:378	a combination of copper sulfate and ascorbic acid (CuAsc)	366:422	BACKGROUND Poly(N-isopropylacrylamide) conjugated hyaluronan (HA-pN), a brush-like copolymer system which serves as a polymer vehicle for cellular and drug delivery, has been previously synthesized via the copper catalyzed azide-alkyne reaction (CuAAC) using a combination of copper sulfate and ascorbic acid (CuAsc) as the catalytic system of choice.
25818153	1	3	theme	acid	411:414	arg1	system					441:446	the catalytic system	427:446	the catalytic system of choice	427:456	BACKGROUND Poly(N-isopropylacrylamide) conjugated hyaluronan (HA-pN), a brush-like copolymer system which serves as a polymer vehicle for cellular and drug delivery, has been previously synthesized via the copper catalyzed azide-alkyne reaction (CuAAC) using a combination of copper sulfate and ascorbic acid (CuAsc) as the catalytic system of choice.
25818153	7	4	theme	final	1434:1438	arg1	polymers					1440:1447	the final polymers	1430:1447	the final polymers	1430:1447	Despite purification via chelation and/or dialysis, residual copper was present in similar concentrations in the final polymers.
25818153	1	5	theme	azide-alkyne	330:341	arg1	CuAAC					353:357	CuAAC	353:357	CuAAC	353:357	BACKGROUND Poly(N-isopropylacrylamide) conjugated hyaluronan (HA-pN), a brush-like copolymer system which serves as a polymer vehicle for cellular and drug delivery, has been previously synthesized via the copper catalyzed azide-alkyne reaction (CuAAC) using a combination of copper sulfate and ascorbic acid (CuAsc) as the catalytic system of choice.
25818153	1	5	theme	azide-alkyne	330:341	arg1	reaction					343:350	the copper catalyzed azide-alkyne reaction	309:350	the copper catalyzed azide-alkyne reaction (CuAAC) using a combination of copper sulfate and ascorbic acid (CuAsc) as the catalytic system of choice	309:456	BACKGROUND Poly(N-isopropylacrylamide) conjugated hyaluronan (HA-pN), a brush-like copolymer system which serves as a polymer vehicle for cellular and drug delivery, has been previously synthesized via the copper catalyzed azide-alkyne reaction (CuAAC) using a combination of copper sulfate and ascorbic acid (CuAsc) as the catalytic system of choice.
25818153	2	6	contain	containing	550:559	arg1	copper					489:494	Bromotris(triphenylphosphine) copper(I)	459:497	Bromotris(triphenylphosphine) copper(I) (CuBr(PPh3)3)	459:511	Bromotris(triphenylphosphine) copper(I) (CuBr(PPh3)3) is an alternative catalytic compound containing a phosphorous ligand which stabilizes copper in the +1 oxidative state in aqueous solvents and can be employed at true catalyst concentrations.
25818153	2	6	contain	containing	550:559	arg1	compound					541:548	an alternative catalytic compound	516:548	an alternative catalytic compound containing a phosphorous ligand which stabilizes copper in the +1 oxidative state in aqueous solvents and can be employed at true catalyst concentrations	516:702	Bromotris(triphenylphosphine) copper(I) (CuBr(PPh3)3) is an alternative catalytic compound containing a phosphorous ligand which stabilizes copper in the +1 oxidative state in aqueous solvents and can be employed at true catalyst concentrations.
25818153	2	6	contain	containing	550:559	arg2	ligand					575:580	a phosphorous ligand	561:580	a phosphorous ligand which stabilizes copper in the +1 oxidative state in aqueous solvents and can be employed at true catalyst concentrations	561:702	Bromotris(triphenylphosphine) copper(I) (CuBr(PPh3)3) is an alternative catalytic compound containing a phosphorous ligand which stabilizes copper in the +1 oxidative state in aqueous solvents and can be employed at true catalyst concentrations.
25818153	1	7	theme	cellular	245:252	arg1	delivery					263:270	cellular and drug delivery	245:270	delivery	263:270	BACKGROUND Poly(N-isopropylacrylamide) conjugated hyaluronan (HA-pN), a brush-like copolymer system which serves as a polymer vehicle for cellular and drug delivery, has been previously synthesized via the copper catalyzed azide-alkyne reaction (CuAAC) using a combination of copper sulfate and ascorbic acid (CuAsc) as the catalytic system of choice.
25818153	7	8	from	present	1393:1399	arg1	concentrations					1412:1425	similar concentrations	1404:1425	similar concentrations in the final polymers	1404:1447	Despite purification via chelation and/or dialysis, residual copper was present in similar concentrations in the final polymers.
25818153	3	9	theme	thermoresponsive	829:844	arg1	compositions					846:857	thermoresponsive compositions and 3) in being extracted from the polymeric compositions	829:915	thermoresponsive compositions and 3) in being extracted from the polymeric compositions	829:915	OBJECTIVE CuAsc and CuBr(PPh3)3 were compared for their efficiency; 1) in the synthesis of HA-pN via CuAAC; 2) in producing thermoresponsive compositions and 3) in being extracted from the polymeric compositions.
25818153	2	10	theme	phosphorous	563:573	arg1	ligand					575:580	a phosphorous ligand	561:580	a phosphorous ligand which stabilizes copper in the +1 oxidative state in aqueous solvents and can be employed at true catalyst concentrations	561:702	Bromotris(triphenylphosphine) copper(I) (CuBr(PPh3)3) is an alternative catalytic compound containing a phosphorous ligand which stabilizes copper in the +1 oxidative state in aqueous solvents and can be employed at true catalyst concentrations.
25818153	2	11	theme	true	675:678	arg1	concentrations					689:702	true catalyst concentrations	675:702	true catalyst concentrations	675:702	Bromotris(triphenylphosphine) copper(I) (CuBr(PPh3)3) is an alternative catalytic compound containing a phosphorous ligand which stabilizes copper in the +1 oxidative state in aqueous solvents and can be employed at true catalyst concentrations.
25818153	2	12	theme	triphenylphosphine	469:486	arg1	PPh3					505:508	CuBr(PPh3)3	500:510	CuBr(PPh3)3	500:510	Bromotris(triphenylphosphine) copper(I) (CuBr(PPh3)3) is an alternative catalytic compound containing a phosphorous ligand which stabilizes copper in the +1 oxidative state in aqueous solvents and can be employed at true catalyst concentrations.
25818153	2	12	theme	triphenylphosphine	469:486	arg1	compound					541:548	an alternative catalytic compound	516:548	an alternative catalytic compound containing a phosphorous ligand which stabilizes copper in the +1 oxidative state in aqueous solvents and can be employed at true catalyst concentrations	516:702	Bromotris(triphenylphosphine) copper(I) (CuBr(PPh3)3) is an alternative catalytic compound containing a phosphorous ligand which stabilizes copper in the +1 oxidative state in aqueous solvents and can be employed at true catalyst concentrations.
25818153	2	12	theme	triphenylphosphine	469:486	arg1	copper					489:494	Bromotris(triphenylphosphine) copper(I)	459:497	Bromotris(triphenylphosphine) copper(I) (CuBr(PPh3)3)	459:511	Bromotris(triphenylphosphine) copper(I) (CuBr(PPh3)3) is an alternative catalytic compound containing a phosphorous ligand which stabilizes copper in the +1 oxidative state in aqueous solvents and can be employed at true catalyst concentrations.
25818153	8	13	dep	driven	1475:1480	arg1	CuAAC					1469:1473	CuAAC	1469:1473	CuAAC	1469:1473	CONCLUSIONS In the CuAAC driven copolymer synthesis of the HA-pN, CuBr(PPh3)3 is a better catalyst than CuAsc.
25818153	4	14	dep	METHODS	918:924	arg1	carried					967:973	carried	967:973	was carried out under strict Schlenk conditions, then characterized by ATR-FTIR, 1H NMR, ICP-SFMS, and rheological measurements	963:1089	METHODS The synthesis of the brush copolymer was carried out under strict Schlenk conditions, then characterized by ATR-FTIR, 1H NMR, ICP-SFMS, and rheological measurements.
25818153	3	15	theme	HA-pN	796:800	arg1	synthesis					783:791	the synthesis	779:791	the synthesis of HA-pN via CuAAC	779:810	OBJECTIVE CuAsc and CuBr(PPh3)3 were compared for their efficiency; 1) in the synthesis of HA-pN via CuAAC; 2) in producing thermoresponsive compositions and 3) in being extracted from the polymeric compositions.
25818153	8	16	from	CONCLUSIONS	1450:1460	arg1	synthesis					1492:1500	the CuAAC driven copolymer synthesis	1465:1500	the CuAAC driven copolymer synthesis of the HA-pN	1465:1513	CONCLUSIONS In the CuAAC driven copolymer synthesis of the HA-pN, CuBr(PPh3)3 is a better catalyst than CuAsc.
25818153	4	17	theme	1H	1044:1045	arg1	NMR					1047:1049	1H NMR	1044:1049	1H NMR	1044:1049	METHODS The synthesis of the brush copolymer was carried out under strict Schlenk conditions, then characterized by ATR-FTIR, 1H NMR, ICP-SFMS, and rheological measurements.
25818153	5	18	theme	true	1168:1171	arg1	concentration					1182:1194	a true catalyst concentration	1166:1194	a true catalyst concentration	1166:1194	RESULTS CuBr(PPh3)3 catalyzed CuAAC leads to better grafting in water, at a true catalyst concentration, compared to CuAsc.
25818153	0	19	theme	catalyst	7:14	arg1	efficiency					16:25	Copper catalyst efficiency	0:25	Copper catalyst efficiency for the CuAAC synthesis of a poly(N-isopropylacrylamide)	0:82	Copper catalyst efficiency for the CuAAC synthesis of a poly(N-isopropylacrylamide) conjugated hyaluronan.
25818153	2	20	theme	catalytic	531:539	arg1	compound					541:548	an alternative catalytic compound	516:548	an alternative catalytic compound containing a phosphorous ligand which stabilizes copper in the +1 oxidative state in aqueous solvents and can be employed at true catalyst concentrations	516:702	Bromotris(triphenylphosphine) copper(I) (CuBr(PPh3)3) is an alternative catalytic compound containing a phosphorous ligand which stabilizes copper in the +1 oxidative state in aqueous solvents and can be employed at true catalyst concentrations.
25818153	2	20	theme	catalytic	531:539	arg1	copper					489:494	Bromotris(triphenylphosphine) copper(I)	459:497	Bromotris(triphenylphosphine) copper(I) (CuBr(PPh3)3)	459:511	Bromotris(triphenylphosphine) copper(I) (CuBr(PPh3)3) is an alternative catalytic compound containing a phosphorous ligand which stabilizes copper in the +1 oxidative state in aqueous solvents and can be employed at true catalyst concentrations.
25818153	2	21	theme	Bromotris	459:467	arg1	PPh3					505:508	CuBr(PPh3)3	500:510	CuBr(PPh3)3	500:510	Bromotris(triphenylphosphine) copper(I) (CuBr(PPh3)3) is an alternative catalytic compound containing a phosphorous ligand which stabilizes copper in the +1 oxidative state in aqueous solvents and can be employed at true catalyst concentrations.
25818153	2	21	theme	Bromotris	459:467	arg1	compound					541:548	an alternative catalytic compound	516:548	an alternative catalytic compound containing a phosphorous ligand which stabilizes copper in the +1 oxidative state in aqueous solvents and can be employed at true catalyst concentrations	516:702	Bromotris(triphenylphosphine) copper(I) (CuBr(PPh3)3) is an alternative catalytic compound containing a phosphorous ligand which stabilizes copper in the +1 oxidative state in aqueous solvents and can be employed at true catalyst concentrations.
25818153	2	21	theme	Bromotris	459:467	arg1	copper					489:494	Bromotris(triphenylphosphine) copper(I)	459:497	Bromotris(triphenylphosphine) copper(I) (CuBr(PPh3)3)	459:511	Bromotris(triphenylphosphine) copper(I) (CuBr(PPh3)3) is an alternative catalytic compound containing a phosphorous ligand which stabilizes copper in the +1 oxidative state in aqueous solvents and can be employed at true catalyst concentrations.
25818153	5	22	theme	catalyst	1173:1180	arg1	concentration					1182:1194	a true catalyst concentration	1166:1194	a true catalyst concentration	1166:1194	RESULTS CuBr(PPh3)3 catalyzed CuAAC leads to better grafting in water, at a true catalyst concentration, compared to CuAsc.
25818153	7	23	theme	residual	1373:1380	arg1	copper					1382:1387	residual copper	1373:1387	residual copper	1373:1387	Despite purification via chelation and/or dialysis, residual copper was present in similar concentrations in the final polymers.
25818153	0	24	theme	Copper	0:5	arg1	efficiency					16:25	Copper catalyst efficiency	0:25	Copper catalyst efficiency for the CuAAC synthesis of a poly(N-isopropylacrylamide)	0:82	Copper catalyst efficiency for the CuAAC synthesis of a poly(N-isopropylacrylamide) conjugated hyaluronan.
25818153	1	25	theme	drug	258:261	arg1	delivery					263:270	cellular and drug delivery	245:270	delivery	263:270	BACKGROUND Poly(N-isopropylacrylamide) conjugated hyaluronan (HA-pN), a brush-like copolymer system which serves as a polymer vehicle for cellular and drug delivery, has been previously synthesized via the copper catalyzed azide-alkyne reaction (CuAAC) using a combination of copper sulfate and ascorbic acid (CuAsc) as the catalytic system of choice.
25818153	2	26	theme	alternative	519:529	arg1	compound					541:548	an alternative catalytic compound	516:548	an alternative catalytic compound containing a phosphorous ligand which stabilizes copper in the +1 oxidative state in aqueous solvents and can be employed at true catalyst concentrations	516:702	Bromotris(triphenylphosphine) copper(I) (CuBr(PPh3)3) is an alternative catalytic compound containing a phosphorous ligand which stabilizes copper in the +1 oxidative state in aqueous solvents and can be employed at true catalyst concentrations.
25818153	2	26	theme	alternative	519:529	arg1	copper					489:494	Bromotris(triphenylphosphine) copper(I)	459:497	Bromotris(triphenylphosphine) copper(I) (CuBr(PPh3)3)	459:511	Bromotris(triphenylphosphine) copper(I) (CuBr(PPh3)3) is an alternative catalytic compound containing a phosphorous ligand which stabilizes copper in the +1 oxidative state in aqueous solvents and can be employed at true catalyst concentrations.
25818153	1	27	theme	catalytic	431:439	arg1	combination					368:378	a combination	366:378	a combination of copper sulfate and ascorbic acid (CuAsc)	366:422	BACKGROUND Poly(N-isopropylacrylamide) conjugated hyaluronan (HA-pN), a brush-like copolymer system which serves as a polymer vehicle for cellular and drug delivery, has been previously synthesized via the copper catalyzed azide-alkyne reaction (CuAAC) using a combination of copper sulfate and ascorbic acid (CuAsc) as the catalytic system of choice.
25818153	1	27	theme	catalytic	431:439	arg1	system					441:446	the catalytic system	427:446	the catalytic system of choice	427:456	BACKGROUND Poly(N-isopropylacrylamide) conjugated hyaluronan (HA-pN), a brush-like copolymer system which serves as a polymer vehicle for cellular and drug delivery, has been previously synthesized via the copper catalyzed azide-alkyne reaction (CuAAC) using a combination of copper sulfate and ascorbic acid (CuAsc) as the catalytic system of choice.
25818153	7	28	theme	similar	1404:1410	arg1	concentrations					1412:1425	similar concentrations	1404:1425	similar concentrations in the final polymers	1404:1447	Despite purification via chelation and/or dialysis, residual copper was present in similar concentrations in the final polymers.
25818153	2	29	from	state	626:630	arg1	solvents					643:650	aqueous solvents	635:650	aqueous solvents	635:650	Bromotris(triphenylphosphine) copper(I) (CuBr(PPh3)3) is an alternative catalytic compound containing a phosphorous ligand which stabilizes copper in the +1 oxidative state in aqueous solvents and can be employed at true catalyst concentrations.
25818153	6	30	theme	mechanical	1275:1284	arg1	stiffness					1286:1294	mechanical stiffness	1275:1294	mechanical stiffness	1275:1294	Polymeric solutions exhibited similar traits of increasing mechanical stiffness with rising temperature.
25818153	7	31	from	concentrations	1412:1425	arg1	present					1393:1399	present	1393:1399	present	1393:1399	Despite purification via chelation and/or dialysis, residual copper was present in similar concentrations in the final polymers.
25818153	7	31	from	concentrations	1412:1425	arg1	polymers					1440:1447	the final polymers	1430:1447	the final polymers	1430:1447	Despite purification via chelation and/or dialysis, residual copper was present in similar concentrations in the final polymers.
25818153	3	32	theme	polymeric	894:902	arg1	compositions					904:915	the polymeric compositions	890:915	the polymeric compositions	890:915	OBJECTIVE CuAsc and CuBr(PPh3)3 were compared for their efficiency; 1) in the synthesis of HA-pN via CuAAC; 2) in producing thermoresponsive compositions and 3) in being extracted from the polymeric compositions.
25818153	3	33	dep	compared	742:749	arg1	1					773:773	1	773:773	1	773:773	OBJECTIVE CuAsc and CuBr(PPh3)3 were compared for their efficiency; 1) in the synthesis of HA-pN via CuAAC; 2) in producing thermoresponsive compositions and 3) in being extracted from the polymeric compositions.
25818153	3	33	dep	compared	742:749	arg1	2					813:813	2	813:813	2	813:813	OBJECTIVE CuAsc and CuBr(PPh3)3 were compared for their efficiency; 1) in the synthesis of HA-pN via CuAAC; 2) in producing thermoresponsive compositions and 3) in being extracted from the polymeric compositions.
25818153	1	34	theme	brush-like	179:188	arg1	hyaluronan					157:166	BACKGROUND Poly(N-isopropylacrylamide) conjugated hyaluronan	107:166	BACKGROUND Poly(N-isopropylacrylamide) conjugated hyaluronan (HA-pN)	107:174	BACKGROUND Poly(N-isopropylacrylamide) conjugated hyaluronan (HA-pN), a brush-like copolymer system which serves as a polymer vehicle for cellular and drug delivery, has been previously synthesized via the copper catalyzed azide-alkyne reaction (CuAAC) using a combination of copper sulfate and ascorbic acid (CuAsc) as the catalytic system of choice.
25818153	1	34	theme	brush-like	179:188	arg1	system					200:205	a brush-like copolymer system	177:205	a brush-like copolymer system which serves as a polymer vehicle for cellular and drug delivery	177:270	BACKGROUND Poly(N-isopropylacrylamide) conjugated hyaluronan (HA-pN), a brush-like copolymer system which serves as a polymer vehicle for cellular and drug delivery, has been previously synthesized via the copper catalyzed azide-alkyne reaction (CuAAC) using a combination of copper sulfate and ascorbic acid (CuAsc) as the catalytic system of choice.
25818153	1	34	theme	brush-like	179:188	arg1	vehicle					233:239	a polymer vehicle	223:239	a polymer vehicle for cellular and drug delivery	223:270	BACKGROUND Poly(N-isopropylacrylamide) conjugated hyaluronan (HA-pN), a brush-like copolymer system which serves as a polymer vehicle for cellular and drug delivery, has been previously synthesized via the copper catalyzed azide-alkyne reaction (CuAAC) using a combination of copper sulfate and ascorbic acid (CuAsc) as the catalytic system of choice.
25818153	4	35	theme	ATR-FTIR	1034:1041	arg1	NMR					1047:1049	1H NMR	1044:1049	1H NMR	1044:1049	METHODS The synthesis of the brush copolymer was carried out under strict Schlenk conditions, then characterized by ATR-FTIR, 1H NMR, ICP-SFMS, and rheological measurements.
25818153	4	36	theme	strict	985:990	arg1	conditions					1000:1009	strict Schlenk conditions	985:1009	strict Schlenk conditions	985:1009	METHODS The synthesis of the brush copolymer was carried out under strict Schlenk conditions, then characterized by ATR-FTIR, 1H NMR, ICP-SFMS, and rheological measurements.
25818153	1	37	theme	copolymer	190:198	arg1	hyaluronan					157:166	BACKGROUND Poly(N-isopropylacrylamide) conjugated hyaluronan	107:166	BACKGROUND Poly(N-isopropylacrylamide) conjugated hyaluronan (HA-pN)	107:174	BACKGROUND Poly(N-isopropylacrylamide) conjugated hyaluronan (HA-pN), a brush-like copolymer system which serves as a polymer vehicle for cellular and drug delivery, has been previously synthesized via the copper catalyzed azide-alkyne reaction (CuAAC) using a combination of copper sulfate and ascorbic acid (CuAsc) as the catalytic system of choice.
25818153	1	37	theme	copolymer	190:198	arg1	system					200:205	a brush-like copolymer system	177:205	a brush-like copolymer system which serves as a polymer vehicle for cellular and drug delivery	177:270	BACKGROUND Poly(N-isopropylacrylamide) conjugated hyaluronan (HA-pN), a brush-like copolymer system which serves as a polymer vehicle for cellular and drug delivery, has been previously synthesized via the copper catalyzed azide-alkyne reaction (CuAAC) using a combination of copper sulfate and ascorbic acid (CuAsc) as the catalytic system of choice.
25818153	1	37	theme	copolymer	190:198	arg1	vehicle					233:239	a polymer vehicle	223:239	a polymer vehicle for cellular and drug delivery	223:270	BACKGROUND Poly(N-isopropylacrylamide) conjugated hyaluronan (HA-pN), a brush-like copolymer system which serves as a polymer vehicle for cellular and drug delivery, has been previously synthesized via the copper catalyzed azide-alkyne reaction (CuAAC) using a combination of copper sulfate and ascorbic acid (CuAsc) as the catalytic system of choice.
25818153	0	38	theme	CuAAC	35:39	arg1	synthesis					41:49	the CuAAC synthesis	31:49	the CuAAC synthesis of a poly(N-isopropylacrylamide)	31:82	Copper catalyst efficiency for the CuAAC synthesis of a poly(N-isopropylacrylamide) conjugated hyaluronan.
25818153	4	39	theme	Schlenk	992:998	arg1	conditions					1000:1009	strict Schlenk conditions	985:1009	strict Schlenk conditions	985:1009	METHODS The synthesis of the brush copolymer was carried out under strict Schlenk conditions, then characterized by ATR-FTIR, 1H NMR, ICP-SFMS, and rheological measurements.
25818153	7	40	located	present	1393:1399	arg1	concentrations					1412:1425	similar concentrations	1404:1425	similar concentrations in the final polymers	1404:1447	Despite purification via chelation and/or dialysis, residual copper was present in similar concentrations in the final polymers.
25818153	7	40	located	present	1393:1399	arg2	copper					1382:1387	residual copper	1373:1387	residual copper	1373:1387	Despite purification via chelation and/or dialysis, residual copper was present in similar concentrations in the final polymers.
25818153	2	41	theme	catalyst	680:687	arg1	concentrations					689:702	true catalyst concentrations	675:702	true catalyst concentrations	675:702	Bromotris(triphenylphosphine) copper(I) (CuBr(PPh3)3) is an alternative catalytic compound containing a phosphorous ligand which stabilizes copper in the +1 oxidative state in aqueous solvents and can be employed at true catalyst concentrations.
25818153	7	42	attach	present	1393:1399	arg1	concentrations					1412:1425	similar concentrations	1404:1425	similar concentrations in the final polymers	1404:1447	Despite purification via chelation and/or dialysis, residual copper was present in similar concentrations in the final polymers.
25818153	7	42	attach	present	1393:1399	arg2	copper					1382:1387	residual copper	1373:1387	residual copper	1373:1387	Despite purification via chelation and/or dialysis, residual copper was present in similar concentrations in the final polymers.
25818153	8	43	theme	CuBr	1516:1519	arg1	PPh3					1521:1524	CuBr(PPh3)3	1516:1526	CuBr(PPh3)3	1516:1526	CONCLUSIONS In the CuAAC driven copolymer synthesis of the HA-pN, CuBr(PPh3)3 is a better catalyst than CuAsc.
25818153	8	43	theme	CuBr	1516:1519	arg1	catalyst					1540:1547	a better catalyst	1531:1547	a better catalyst than CuAsc	1531:1558	CONCLUSIONS In the CuAAC driven copolymer synthesis of the HA-pN, CuBr(PPh3)3 is a better catalyst than CuAsc.
25818153	8	44	theme	copolymer	1482:1490	arg1	synthesis					1492:1500	the CuAAC driven copolymer synthesis	1465:1500	the CuAAC driven copolymer synthesis of the HA-pN	1465:1513	CONCLUSIONS In the CuAAC driven copolymer synthesis of the HA-pN, CuBr(PPh3)3 is a better catalyst than CuAsc.
25818153	6	45	theme	similar	1246:1252	arg1	traits					1254:1259	similar traits	1246:1259	similar traits of increasing mechanical stiffness with rising temperature	1246:1318	Polymeric solutions exhibited similar traits of increasing mechanical stiffness with rising temperature.
25818153	3	46	theme	OBJECTIVE	705:713	arg1	CuAsc					715:719	OBJECTIVE CuAsc	705:719	OBJECTIVE CuAsc	705:719	OBJECTIVE CuAsc and CuBr(PPh3)3 were compared for their efficiency; 1) in the synthesis of HA-pN via CuAAC; 2) in producing thermoresponsive compositions and 3) in being extracted from the polymeric compositions.
25818153	5	47	dep	RESULTS	1092:1098	arg1	leads					1128:1132	leads	1128:1132	leads	1128:1132	RESULTS CuBr(PPh3)3 catalyzed CuAAC leads to better grafting in water, at a true catalyst concentration, compared to CuAsc.
25818153	5	48	from	grafting	1144:1151	arg1	water					1156:1160	water	1156:1160	water	1156:1160	RESULTS CuBr(PPh3)3 catalyzed CuAAC leads to better grafting in water, at a true catalyst concentration, compared to CuAsc.
25818153	1	49	theme	choice	451:456	arg1	combination					368:378	a combination	366:378	a combination of copper sulfate and ascorbic acid (CuAsc)	366:422	BACKGROUND Poly(N-isopropylacrylamide) conjugated hyaluronan (HA-pN), a brush-like copolymer system which serves as a polymer vehicle for cellular and drug delivery, has been previously synthesized via the copper catalyzed azide-alkyne reaction (CuAAC) using a combination of copper sulfate and ascorbic acid (CuAsc) as the catalytic system of choice.
25818153	1	49	theme	choice	451:456	arg1	system					441:446	the catalytic system	427:446	the catalytic system of choice	427:456	BACKGROUND Poly(N-isopropylacrylamide) conjugated hyaluronan (HA-pN), a brush-like copolymer system which serves as a polymer vehicle for cellular and drug delivery, has been previously synthesized via the copper catalyzed azide-alkyne reaction (CuAAC) using a combination of copper sulfate and ascorbic acid (CuAsc) as the catalytic system of choice.
25818153	1	50	theme	BACKGROUND	107:116	arg1	N-isopropylacrylamide					123:143	N-isopropylacrylamide	123:143	N-isopropylacrylamide	123:143	BACKGROUND Poly(N-isopropylacrylamide) conjugated hyaluronan (HA-pN), a brush-like copolymer system which serves as a polymer vehicle for cellular and drug delivery, has been previously synthesized via the copper catalyzed azide-alkyne reaction (CuAAC) using a combination of copper sulfate and ascorbic acid (CuAsc) as the catalytic system of choice.
25818153	1	50	theme	BACKGROUND	107:116	arg1	Poly					118:121	BACKGROUND Poly	107:121	BACKGROUND Poly(N-isopropylacrylamide) conjugated hyaluronan (HA-pN)	107:174	BACKGROUND Poly(N-isopropylacrylamide) conjugated hyaluronan (HA-pN), a brush-like copolymer system which serves as a polymer vehicle for cellular and drug delivery, has been previously synthesized via the copper catalyzed azide-alkyne reaction (CuAAC) using a combination of copper sulfate and ascorbic acid (CuAsc) as the catalytic system of choice.
25818153	5	51	theme	better	1137:1142	arg1	grafting					1144:1151	better grafting	1137:1151	better grafting in water	1137:1160	RESULTS CuBr(PPh3)3 catalyzed CuAAC leads to better grafting in water, at a true catalyst concentration, compared to CuAsc.
25818153	8	52	theme	HA-pN	1509:1513	arg1	synthesis					1492:1500	the CuAAC driven copolymer synthesis	1465:1500	the CuAAC driven copolymer synthesis of the HA-pN	1465:1513	CONCLUSIONS In the CuAAC driven copolymer synthesis of the HA-pN, CuBr(PPh3)3 is a better catalyst than CuAsc.
25818153	8	53	theme	better	1533:1538	arg1	PPh3					1521:1524	CuBr(PPh3)3	1516:1526	CuBr(PPh3)3	1516:1526	CONCLUSIONS In the CuAAC driven copolymer synthesis of the HA-pN, CuBr(PPh3)3 is a better catalyst than CuAsc.
25818153	8	53	theme	better	1533:1538	arg1	catalyst					1540:1547	a better catalyst	1531:1547	a better catalyst than CuAsc	1531:1558	CONCLUSIONS In the CuAAC driven copolymer synthesis of the HA-pN, CuBr(PPh3)3 is a better catalyst than CuAsc.
25818153	1	54	theme	copper	383:388	arg1	sulfate					390:396	copper sulfate	383:396	copper sulfate	383:396	BACKGROUND Poly(N-isopropylacrylamide) conjugated hyaluronan (HA-pN), a brush-like copolymer system which serves as a polymer vehicle for cellular and drug delivery, has been previously synthesized via the copper catalyzed azide-alkyne reaction (CuAAC) using a combination of copper sulfate and ascorbic acid (CuAsc) as the catalytic system of choice.
25818153	2	55	theme	oxidative	616:624	arg1	state					626:630	the +1 oxidative state	609:630	the +1 oxidative state in aqueous solvents	609:650	Bromotris(triphenylphosphine) copper(I) (CuBr(PPh3)3) is an alternative catalytic compound containing a phosphorous ligand which stabilizes copper in the +1 oxidative state in aqueous solvents and can be employed at true catalyst concentrations.
25818153	1	56	theme	Poly	118:121	arg1	system					200:205	a brush-like copolymer system	177:205	a brush-like copolymer system which serves as a polymer vehicle for cellular and drug delivery	177:270	BACKGROUND Poly(N-isopropylacrylamide) conjugated hyaluronan (HA-pN), a brush-like copolymer system which serves as a polymer vehicle for cellular and drug delivery, has been previously synthesized via the copper catalyzed azide-alkyne reaction (CuAAC) using a combination of copper sulfate and ascorbic acid (CuAsc) as the catalytic system of choice.
25818153	1	56	theme	Poly	118:121	arg1	HA-pN					169:173	HA-pN	169:173	HA-pN	169:173	BACKGROUND Poly(N-isopropylacrylamide) conjugated hyaluronan (HA-pN), a brush-like copolymer system which serves as a polymer vehicle for cellular and drug delivery, has been previously synthesized via the copper catalyzed azide-alkyne reaction (CuAAC) using a combination of copper sulfate and ascorbic acid (CuAsc) as the catalytic system of choice.
25818153	1	56	theme	Poly	118:121	arg1	hyaluronan					157:166	BACKGROUND Poly(N-isopropylacrylamide) conjugated hyaluronan	107:166	BACKGROUND Poly(N-isopropylacrylamide) conjugated hyaluronan (HA-pN)	107:174	BACKGROUND Poly(N-isopropylacrylamide) conjugated hyaluronan (HA-pN), a brush-like copolymer system which serves as a polymer vehicle for cellular and drug delivery, has been previously synthesized via the copper catalyzed azide-alkyne reaction (CuAAC) using a combination of copper sulfate and ascorbic acid (CuAsc) as the catalytic system of choice.
25818153	4	57	theme	brush	947:951	arg1	copolymer					953:961	the brush copolymer	943:961	the brush copolymer	943:961	METHODS The synthesis of the brush copolymer was carried out under strict Schlenk conditions, then characterized by ATR-FTIR, 1H NMR, ICP-SFMS, and rheological measurements.
25818153	4	58	theme	rheological	1066:1076	arg1	measurements					1078:1089	rheological measurements	1066:1089	rheological measurements	1066:1089	METHODS The synthesis of the brush copolymer was carried out under strict Schlenk conditions, then characterized by ATR-FTIR, 1H NMR, ICP-SFMS, and rheological measurements.
25818153	1	59	theme	sulfate	390:396	arg1	combination					368:378	a combination	366:378	a combination of copper sulfate and ascorbic acid (CuAsc)	366:422	BACKGROUND Poly(N-isopropylacrylamide) conjugated hyaluronan (HA-pN), a brush-like copolymer system which serves as a polymer vehicle for cellular and drug delivery, has been previously synthesized via the copper catalyzed azide-alkyne reaction (CuAAC) using a combination of copper sulfate and ascorbic acid (CuAsc) as the catalytic system of choice.
25818153	1	59	theme	sulfate	390:396	arg1	system					441:446	the catalytic system	427:446	the catalytic system of choice	427:456	BACKGROUND Poly(N-isopropylacrylamide) conjugated hyaluronan (HA-pN), a brush-like copolymer system which serves as a polymer vehicle for cellular and drug delivery, has been previously synthesized via the copper catalyzed azide-alkyne reaction (CuAAC) using a combination of copper sulfate and ascorbic acid (CuAsc) as the catalytic system of choice.
25818153	2	60	theme	+1	613:614	arg1	state					626:630	the +1 oxidative state	609:630	the +1 oxidative state in aqueous solvents	609:650	Bromotris(triphenylphosphine) copper(I) (CuBr(PPh3)3) is an alternative catalytic compound containing a phosphorous ligand which stabilizes copper in the +1 oxidative state in aqueous solvents and can be employed at true catalyst concentrations.
25818153	5	61	theme	catalyzed	1112:1120	arg1	CuAAC					1122:1126	CuBr(PPh3)3 catalyzed CuAAC	1100:1126	CuBr(PPh3)3 catalyzed CuAAC	1100:1126	RESULTS CuBr(PPh3)3 catalyzed CuAAC leads to better grafting in water, at a true catalyst concentration, compared to CuAsc.
25818153	3	62	theme	CuBr	725:728	arg1	PPh3					730:733	CuBr(PPh3)3	725:735	CuBr(PPh3)3	725:735	OBJECTIVE CuAsc and CuBr(PPh3)3 were compared for their efficiency; 1) in the synthesis of HA-pN via CuAAC; 2) in producing thermoresponsive compositions and 3) in being extracted from the polymeric compositions.
25818153	2	63	theme	CuBr	500:503	arg1	PPh3					505:508	CuBr(PPh3)3	500:510	CuBr(PPh3)3	500:510	Bromotris(triphenylphosphine) copper(I) (CuBr(PPh3)3) is an alternative catalytic compound containing a phosphorous ligand which stabilizes copper in the +1 oxidative state in aqueous solvents and can be employed at true catalyst concentrations.
25818153	2	63	theme	CuBr	500:503	arg1	copper					489:494	Bromotris(triphenylphosphine) copper(I)	459:497	Bromotris(triphenylphosphine) copper(I) (CuBr(PPh3)3)	459:511	Bromotris(triphenylphosphine) copper(I) (CuBr(PPh3)3) is an alternative catalytic compound containing a phosphorous ligand which stabilizes copper in the +1 oxidative state in aqueous solvents and can be employed at true catalyst concentrations.
25818153	3	64	dep	extracted	875:883	arg1	3					863:863	3	863:863	3	863:863	OBJECTIVE CuAsc and CuBr(PPh3)3 were compared for their efficiency; 1) in the synthesis of HA-pN via CuAAC; 2) in producing thermoresponsive compositions and 3) in being extracted from the polymeric compositions.
25818153	0	65	theme	poly	56:59	arg1	synthesis					41:49	the CuAAC synthesis	31:49	the CuAAC synthesis of a poly(N-isopropylacrylamide)	31:82	Copper catalyst efficiency for the CuAAC synthesis of a poly(N-isopropylacrylamide) conjugated hyaluronan.
25818153	1	66	theme	ascorbic	402:409	arg1	CuAsc					417:421	CuAsc	417:421	CuAsc	417:421	BACKGROUND Poly(N-isopropylacrylamide) conjugated hyaluronan (HA-pN), a brush-like copolymer system which serves as a polymer vehicle for cellular and drug delivery, has been previously synthesized via the copper catalyzed azide-alkyne reaction (CuAAC) using a combination of copper sulfate and ascorbic acid (CuAsc) as the catalytic system of choice.
25818153	1	66	theme	ascorbic	402:409	arg1	acid					411:414	ascorbic acid	402:414	ascorbic acid (CuAsc)	402:422	BACKGROUND Poly(N-isopropylacrylamide) conjugated hyaluronan (HA-pN), a brush-like copolymer system which serves as a polymer vehicle for cellular and drug delivery, has been previously synthesized via the copper catalyzed azide-alkyne reaction (CuAAC) using a combination of copper sulfate and ascorbic acid (CuAsc) as the catalytic system of choice.
25818153	2	67	from	copper	599:604	arg1	state					626:630	the +1 oxidative state	609:630	the +1 oxidative state in aqueous solvents	609:650	Bromotris(triphenylphosphine) copper(I) (CuBr(PPh3)3) is an alternative catalytic compound containing a phosphorous ligand which stabilizes copper in the +1 oxidative state in aqueous solvents and can be employed at true catalyst concentrations.
25818153	4	68	theme	copolymer	953:961	arg1	synthesis					930:938	The synthesis	926:938	The synthesis of the brush copolymer	926:961	METHODS The synthesis of the brush copolymer was carried out under strict Schlenk conditions, then characterized by ATR-FTIR, 1H NMR, ICP-SFMS, and rheological measurements.
25818153	5	69	theme	CuBr	1100:1103	arg1	CuAAC					1122:1126	CuBr(PPh3)3 catalyzed CuAAC	1100:1126	CuBr(PPh3)3 catalyzed CuAAC	1100:1126	RESULTS CuBr(PPh3)3 catalyzed CuAAC leads to better grafting in water, at a true catalyst concentration, compared to CuAsc.
25818153	6	70	theme	Polymeric	1216:1224	arg1	solutions					1226:1234	Polymeric solutions	1216:1234	Polymeric solutions	1216:1234	Polymeric solutions exhibited similar traits of increasing mechanical stiffness with rising temperature.
25818153	8	71	theme	driven	1475:1480	arg1	synthesis					1492:1500	the CuAAC driven copolymer synthesis	1465:1500	the CuAAC driven copolymer synthesis of the HA-pN	1465:1513	CONCLUSIONS In the CuAAC driven copolymer synthesis of the HA-pN, CuBr(PPh3)3 is a better catalyst than CuAsc.
25818153	1	72	theme	conjugated	146:155	arg1	system					200:205	a brush-like copolymer system	177:205	a brush-like copolymer system which serves as a polymer vehicle for cellular and drug delivery	177:270	BACKGROUND Poly(N-isopropylacrylamide) conjugated hyaluronan (HA-pN), a brush-like copolymer system which serves as a polymer vehicle for cellular and drug delivery, has been previously synthesized via the copper catalyzed azide-alkyne reaction (CuAAC) using a combination of copper sulfate and ascorbic acid (CuAsc) as the catalytic system of choice.
25818153	1	72	theme	conjugated	146:155	arg1	HA-pN					169:173	HA-pN	169:173	HA-pN	169:173	BACKGROUND Poly(N-isopropylacrylamide) conjugated hyaluronan (HA-pN), a brush-like copolymer system which serves as a polymer vehicle for cellular and drug delivery, has been previously synthesized via the copper catalyzed azide-alkyne reaction (CuAAC) using a combination of copper sulfate and ascorbic acid (CuAsc) as the catalytic system of choice.
25818153	1	72	theme	conjugated	146:155	arg1	hyaluronan					157:166	BACKGROUND Poly(N-isopropylacrylamide) conjugated hyaluronan	107:166	BACKGROUND Poly(N-isopropylacrylamide) conjugated hyaluronan (HA-pN)	107:174	BACKGROUND Poly(N-isopropylacrylamide) conjugated hyaluronan (HA-pN), a brush-like copolymer system which serves as a polymer vehicle for cellular and drug delivery, has been previously synthesized via the copper catalyzed azide-alkyne reaction (CuAAC) using a combination of copper sulfate and ascorbic acid (CuAsc) as the catalytic system of choice.
25818153	5	73	theme	PPh3	1105:1108	arg1	CuAAC					1122:1126	CuBr(PPh3)3 catalyzed CuAAC	1100:1126	CuBr(PPh3)3 catalyzed CuAAC	1100:1126	RESULTS CuBr(PPh3)3 catalyzed CuAAC leads to better grafting in water, at a true catalyst concentration, compared to CuAsc.
25818153	1	74	theme	polymer	225:231	arg1	system					200:205	a brush-like copolymer system	177:205	a brush-like copolymer system which serves as a polymer vehicle for cellular and drug delivery	177:270	BACKGROUND Poly(N-isopropylacrylamide) conjugated hyaluronan (HA-pN), a brush-like copolymer system which serves as a polymer vehicle for cellular and drug delivery, has been previously synthesized via the copper catalyzed azide-alkyne reaction (CuAAC) using a combination of copper sulfate and ascorbic acid (CuAsc) as the catalytic system of choice.
25818153	1	74	theme	polymer	225:231	arg1	vehicle					233:239	a polymer vehicle	223:239	a polymer vehicle for cellular and drug delivery	223:270	BACKGROUND Poly(N-isopropylacrylamide) conjugated hyaluronan (HA-pN), a brush-like copolymer system which serves as a polymer vehicle for cellular and drug delivery, has been previously synthesized via the copper catalyzed azide-alkyne reaction (CuAAC) using a combination of copper sulfate and ascorbic acid (CuAsc) as the catalytic system of choice.
26454111	0	0	theme	CCl₄-induced	105:116	arg1	injury					126:131	CCl₄-induced hepatic injury	105:131	CCl₄-induced hepatic injury in mice	105:139	Hepatoprotective effects of polysaccharide isolated from Agaricus bisporus industrial wastewater against CCl₄-induced hepatic injury in mice.
26454111	4	1	theme	average	739:745	arg1	weights					757:763	average molecular weights	739:763	average molecular weights of 336, 12.8, 330 and 15.8kDa, respectively	739:807	Results of ultraviolet spectra analysis and molecular weight determination showed that Abnp1001, Abnp1002, Abap1001 and Abap1002 were uniform with average molecular weights of 336, 12.8, 330 and 15.8kDa, respectively.
26454111	1	2	theme	canned	178:183	arg1	mushroom					185:192	canned mushroom	178:192	canned mushroom (Agaricus bisporus)	178:212	During the industrial production of canned mushroom (Agaricus bisporus), a large quantity of wastewater is produced.
26454111	4	3	theme	spectra	615:621	arg1	analysis					623:630	analysis	623:630	analysis	623:630	Results of ultraviolet spectra analysis and molecular weight determination showed that Abnp1001, Abnp1002, Abap1001 and Abap1002 were uniform with average molecular weights of 336, 12.8, 330 and 15.8kDa, respectively.
26454111	4	3	theme	spectra	615:621	arg1	determination					653:665	molecular weight determination	636:665	molecular weight determination	636:665	Results of ultraviolet spectra analysis and molecular weight determination showed that Abnp1001, Abnp1002, Abap1001 and Abap1002 were uniform with average molecular weights of 336, 12.8, 330 and 15.8kDa, respectively.
26454111	4	3	theme	spectra	615:621	arg1	Results					592:598	Results	592:598	Results of ultraviolet spectra analysis and molecular weight determination	592:665	Results of ultraviolet spectra analysis and molecular weight determination showed that Abnp1001, Abnp1002, Abap1001 and Abap1002 were uniform with average molecular weights of 336, 12.8, 330 and 15.8kDa, respectively.
26454111	9	4	theme	acute	1743:1747	arg1	injury					1757:1762	acute hepatic injury	1743:1762	acute hepatic injury	1743:1762	These results indicate that these two polysaccharides had protective effects on acute hepatic injury induced by CCl4 in mice and suggest that the polysaccharides extracted from A. bisporus industrial wastewater might have potential in therapeutics of acute hepatic injury.
26454111	3	5	theme	DEAE	509:512	arg1	ultrafiltration					492:506	ultrafiltration	492:506	ultrafiltration	492:506	From this wastewater, four polysaccharide components (Abnp1001, Abnp1002, Abap1001, and Abap1002) with hepatic-protective activity were isolated by ultrafiltration, DEAE cellulose-52 chromatography and Sephadex G-200 size-exclusion chromatography.
26454111	3	5	theme	DEAE	509:512	arg1	chromatography					527:540	DEAE cellulose-52 chromatography	509:540	DEAE cellulose-52 chromatography	509:540	From this wastewater, four polysaccharide components (Abnp1001, Abnp1002, Abap1001, and Abap1002) with hepatic-protective activity were isolated by ultrafiltration, DEAE cellulose-52 chromatography and Sephadex G-200 size-exclusion chromatography.
26454111	9	6	theme	acute	1572:1576	arg1	injury					1586:1591	acute hepatic injury	1572:1591	acute hepatic injury induced by CCl4 in mice	1572:1615	These results indicate that these two polysaccharides had protective effects on acute hepatic injury induced by CCl4 in mice and suggest that the polysaccharides extracted from A. bisporus industrial wastewater might have potential in therapeutics of acute hepatic injury.
26454111	4	7	theme	ultraviolet	603:613	arg1	spectra					615:621	ultraviolet spectra	603:621	ultraviolet spectra	603:621	Results of ultraviolet spectra analysis and molecular weight determination showed that Abnp1001, Abnp1002, Abap1001 and Abap1002 were uniform with average molecular weights of 336, 12.8, 330 and 15.8kDa, respectively.
26454111	3	8	dep	components	386:395	arg1	components					386:395	four polysaccharide components	366:395	four polysaccharide components (Abnp1001, Abnp1002, Abap1001, and Abap1002) with hepatic-protective activity	366:473	From this wastewater, four polysaccharide components (Abnp1001, Abnp1002, Abap1001, and Abap1002) with hepatic-protective activity were isolated by ultrafiltration, DEAE cellulose-52 chromatography and Sephadex G-200 size-exclusion chromatography.
26454111	3	8	dep	components	386:395	arg1	Abap1001					418:425	Abap1001	418:425	Abap1001	418:425	From this wastewater, four polysaccharide components (Abnp1001, Abnp1002, Abap1001, and Abap1002) with hepatic-protective activity were isolated by ultrafiltration, DEAE cellulose-52 chromatography and Sephadex G-200 size-exclusion chromatography.
26454111	3	8	dep	components	386:395	arg1	Abap1002					432:439	Abap1002	432:439	Abap1002	432:439	From this wastewater, four polysaccharide components (Abnp1001, Abnp1002, Abap1001, and Abap1002) with hepatic-protective activity were isolated by ultrafiltration, DEAE cellulose-52 chromatography and Sephadex G-200 size-exclusion chromatography.
26454111	3	8	dep	components	386:395	arg1	Abnp1002					408:415	Abnp1002	408:415	Abnp1002	408:415	From this wastewater, four polysaccharide components (Abnp1001, Abnp1002, Abap1001, and Abap1002) with hepatic-protective activity were isolated by ultrafiltration, DEAE cellulose-52 chromatography and Sephadex G-200 size-exclusion chromatography.
26454111	3	8	dep	components	386:395	arg1	Abnp1001					398:405	Abnp1001	398:405	Abnp1001	398:405	From this wastewater, four polysaccharide components (Abnp1001, Abnp1002, Abap1001, and Abap1002) with hepatic-protective activity were isolated by ultrafiltration, DEAE cellulose-52 chromatography and Sephadex G-200 size-exclusion chromatography.
26454111	3	9	theme	G-200	555:559	arg1	ultrafiltration					492:506	ultrafiltration	492:506	ultrafiltration	492:506	From this wastewater, four polysaccharide components (Abnp1001, Abnp1002, Abap1001, and Abap1002) with hepatic-protective activity were isolated by ultrafiltration, DEAE cellulose-52 chromatography and Sephadex G-200 size-exclusion chromatography.
26454111	3	9	theme	G-200	555:559	arg1	chromatography					576:589	Sephadex G-200 size-exclusion chromatography	546:589	Sephadex G-200 size-exclusion chromatography	546:589	From this wastewater, four polysaccharide components (Abnp1001, Abnp1002, Abap1001, and Abap1002) with hepatic-protective activity were isolated by ultrafiltration, DEAE cellulose-52 chromatography and Sephadex G-200 size-exclusion chromatography.
26454111	9	10	theme	A.	1669:1670	arg1	wastewater					1692:1701	A. bisporus industrial wastewater	1669:1701	A. bisporus industrial wastewater	1669:1701	These results indicate that these two polysaccharides had protective effects on acute hepatic injury induced by CCl4 in mice and suggest that the polysaccharides extracted from A. bisporus industrial wastewater might have potential in therapeutics of acute hepatic injury.
26454111	6	11	theme	isolated	1030:1037	arg1	fractions					1039:1047	the isolated fractions	1026:1047	the isolated fractions	1026:1047	Fourier transform-infrared (FT-IR) analysis showed that the isolated fractions were all composed of β-glycoside linkages.
26454111	0	12	theme	hepatic	118:124	arg1	injury					126:131	CCl₄-induced hepatic injury	105:131	CCl₄-induced hepatic injury in mice	105:139	Hepatoprotective effects of polysaccharide isolated from Agaricus bisporus industrial wastewater against CCl₄-induced hepatic injury in mice.
26454111	7	13	theme	hepatic	1194:1200	arg1	injury					1202:1207	CCl4-induced hepatic injury	1181:1207	CCl4-induced hepatic injury in mice	1181:1215	Additionally, the potential hepatoprotective activities of these polysaccharides against CCl4-induced hepatic injury in mice were studied.
26454111	3	14	theme	size-exclusion	561:574	arg1	ultrafiltration					492:506	ultrafiltration	492:506	ultrafiltration	492:506	From this wastewater, four polysaccharide components (Abnp1001, Abnp1002, Abap1001, and Abap1002) with hepatic-protective activity were isolated by ultrafiltration, DEAE cellulose-52 chromatography and Sephadex G-200 size-exclusion chromatography.
26454111	3	14	theme	size-exclusion	561:574	arg1	chromatography					576:589	Sephadex G-200 size-exclusion chromatography	546:589	Sephadex G-200 size-exclusion chromatography	546:589	From this wastewater, four polysaccharide components (Abnp1001, Abnp1002, Abap1001, and Abap1002) with hepatic-protective activity were isolated by ultrafiltration, DEAE cellulose-52 chromatography and Sephadex G-200 size-exclusion chromatography.
26454111	1	15	theme	industrial	153:162	arg1	production					164:173	the industrial production	149:173	the industrial production of canned mushroom (Agaricus bisporus)	149:212	During the industrial production of canned mushroom (Agaricus bisporus), a large quantity of wastewater is produced.
26454111	4	16	dep	Results	592:598	arg1	analysis					623:630	analysis	623:630	analysis	623:630	Results of ultraviolet spectra analysis and molecular weight determination showed that Abnp1001, Abnp1002, Abap1001 and Abap1002 were uniform with average molecular weights of 336, 12.8, 330 and 15.8kDa, respectively.
26454111	4	16	dep	Results	592:598	arg1	Results					592:598	Results	592:598	Results of ultraviolet spectra analysis and molecular weight determination	592:665	Results of ultraviolet spectra analysis and molecular weight determination showed that Abnp1001, Abnp1002, Abap1001 and Abap1002 were uniform with average molecular weights of 336, 12.8, 330 and 15.8kDa, respectively.
26454111	4	16	dep	Results	592:598	arg1	determination					653:665	molecular weight determination	636:665	molecular weight determination	636:665	Results of ultraviolet spectra analysis and molecular weight determination showed that Abnp1001, Abnp1002, Abap1001 and Abap1002 were uniform with average molecular weights of 336, 12.8, 330 and 15.8kDa, respectively.
26454111	7	17	theme	polysaccharides	1157:1171	arg1	activities					1137:1146	the potential hepatoprotective activities	1106:1146	the potential hepatoprotective activities of these polysaccharides against CCl4-induced hepatic injury in mice	1106:1215	Additionally, the potential hepatoprotective activities of these polysaccharides against CCl4-induced hepatic injury in mice were studied.
26454111	9	18	theme	protective	1550:1559	arg1	effects					1561:1567	protective effects	1550:1567	protective effects	1550:1567	These results indicate that these two polysaccharides had protective effects on acute hepatic injury induced by CCl4 in mice and suggest that the polysaccharides extracted from A. bisporus industrial wastewater might have potential in therapeutics of acute hepatic injury.
26454111	9	19	contain	had	1546:1548	arg1	polysaccharides					1530:1544	these two polysaccharides	1520:1544	these two polysaccharides	1520:1544	These results indicate that these two polysaccharides had protective effects on acute hepatic injury induced by CCl4 in mice and suggest that the polysaccharides extracted from A. bisporus industrial wastewater might have potential in therapeutics of acute hepatic injury.
26454111	9	19	contain	had	1546:1548	arg2	effects					1561:1567	protective effects	1550:1567	protective effects	1550:1567	These results indicate that these two polysaccharides had protective effects on acute hepatic injury induced by CCl4 in mice and suggest that the polysaccharides extracted from A. bisporus industrial wastewater might have potential in therapeutics of acute hepatic injury.
26454111	5	20	theme	composition	829:839	arg1	analysis					841:848	The monosaccharide composition analysis	810:848	The monosaccharide composition analysis using gas chromatography (GC)	810:878	The monosaccharide composition analysis using gas chromatography (GC) showed that the four fractions were heteropolysaccharides and mainly composed of glucose.
26454111	3	21	theme	Sephadex	546:553	arg1	ultrafiltration					492:506	ultrafiltration	492:506	ultrafiltration	492:506	From this wastewater, four polysaccharide components (Abnp1001, Abnp1002, Abap1001, and Abap1002) with hepatic-protective activity were isolated by ultrafiltration, DEAE cellulose-52 chromatography and Sephadex G-200 size-exclusion chromatography.
26454111	3	21	theme	Sephadex	546:553	arg1	chromatography					576:589	Sephadex G-200 size-exclusion chromatography	546:589	Sephadex G-200 size-exclusion chromatography	546:589	From this wastewater, four polysaccharide components (Abnp1001, Abnp1002, Abap1001, and Abap1002) with hepatic-protective activity were isolated by ultrafiltration, DEAE cellulose-52 chromatography and Sephadex G-200 size-exclusion chromatography.
26454111	7	22	from	injury	1202:1207	arg1	mice					1212:1215	mice	1212:1215	mice	1212:1215	Additionally, the potential hepatoprotective activities of these polysaccharides against CCl4-induced hepatic injury in mice were studied.
26454111	4	23	theme	12.8	773:776	arg1	weights					757:763	average molecular weights	739:763	average molecular weights of 336, 12.8, 330 and 15.8kDa, respectively	739:807	Results of ultraviolet spectra analysis and molecular weight determination showed that Abnp1001, Abnp1002, Abap1001 and Abap1002 were uniform with average molecular weights of 336, 12.8, 330 and 15.8kDa, respectively.
26454111	8	24	theme	aminotransferase	1323:1338	arg1	concentrations					1346:1359	the alanine aminotransferase (ALT) and aspartate aminotransferase (AST) concentrations	1274:1359	the alanine aminotransferase (ALT) and aspartate aminotransferase (AST) concentrations in serum	1274:1368	Notably, Abnp1002 and Abap1002 could lower the alanine aminotransferase (ALT) and aspartate aminotransferase (AST) concentrations in serum in a dose dependent manner and reduce the hepatocellular degeneration and necrosis, as well as inflammatory infiltration.
26454111	0	25	theme	Hepatoprotective	0:15	arg1	effects					17:23	Hepatoprotective effects	0:23	Hepatoprotective effects of polysaccharide	0:41	Hepatoprotective effects of polysaccharide isolated from Agaricus bisporus industrial wastewater against CCl₄-induced hepatic injury in mice.
26454111	8	26	from	concentrations	1346:1359	arg1	manner					1390:1395	a dose dependent manner	1373:1395	a dose dependent manner	1373:1395	Notably, Abnp1002 and Abap1002 could lower the alanine aminotransferase (ALT) and aspartate aminotransferase (AST) concentrations in serum in a dose dependent manner and reduce the hepatocellular degeneration and necrosis, as well as inflammatory infiltration.
26454111	8	26	from	concentrations	1346:1359	arg1	serum					1364:1368	serum	1364:1368	serum	1364:1368	Notably, Abnp1002 and Abap1002 could lower the alanine aminotransferase (ALT) and aspartate aminotransferase (AST) concentrations in serum in a dose dependent manner and reduce the hepatocellular degeneration and necrosis, as well as inflammatory infiltration.
26454111	1	27	dep	mushroom	185:192	arg1	bisporus					204:211	bisporus	204:211	bisporus	204:211	During the industrial production of canned mushroom (Agaricus bisporus), a large quantity of wastewater is produced.
26454111	8	28	theme	aspartate	1313:1321	arg1	AST					1341:1343	AST	1341:1343	AST	1341:1343	Notably, Abnp1002 and Abap1002 could lower the alanine aminotransferase (ALT) and aspartate aminotransferase (AST) concentrations in serum in a dose dependent manner and reduce the hepatocellular degeneration and necrosis, as well as inflammatory infiltration.
26454111	8	28	theme	aspartate	1313:1321	arg1	aminotransferase					1323:1338	aspartate aminotransferase	1313:1338	aspartate aminotransferase (AST)	1313:1344	Notably, Abnp1002 and Abap1002 could lower the alanine aminotransferase (ALT) and aspartate aminotransferase (AST) concentrations in serum in a dose dependent manner and reduce the hepatocellular degeneration and necrosis, as well as inflammatory infiltration.
26454111	0	29	theme	polysaccharide	28:41	arg1	effects					17:23	Hepatoprotective effects	0:23	Hepatoprotective effects of polysaccharide	0:41	Hepatoprotective effects of polysaccharide isolated from Agaricus bisporus industrial wastewater against CCl₄-induced hepatic injury in mice.
26454111	7	30	theme	hepatoprotective	1120:1135	arg1	activities					1137:1146	the potential hepatoprotective activities	1106:1146	the potential hepatoprotective activities of these polysaccharides against CCl4-induced hepatic injury in mice	1106:1215	Additionally, the potential hepatoprotective activities of these polysaccharides against CCl4-induced hepatic injury in mice were studied.
26454111	3	31	with	components	386:395	arg1	activity					466:473	hepatic-protective activity	447:473	hepatic-protective activity	447:473	From this wastewater, four polysaccharide components (Abnp1001, Abnp1002, Abap1001, and Abap1002) with hepatic-protective activity were isolated by ultrafiltration, DEAE cellulose-52 chromatography and Sephadex G-200 size-exclusion chromatography.
26454111	3	32	theme	hepatic-protective	447:464	arg1	activity					466:473	hepatic-protective activity	447:473	hepatic-protective activity	447:473	From this wastewater, four polysaccharide components (Abnp1001, Abnp1002, Abap1001, and Abap1002) with hepatic-protective activity were isolated by ultrafiltration, DEAE cellulose-52 chromatography and Sephadex G-200 size-exclusion chromatography.
26454111	3	33	theme	polysaccharide	371:384	arg1	components					386:395	four polysaccharide components	366:395	four polysaccharide components (Abnp1001, Abnp1002, Abap1001, and Abap1002) with hepatic-protective activity	366:473	From this wastewater, four polysaccharide components (Abnp1001, Abnp1002, Abap1001, and Abap1002) with hepatic-protective activity were isolated by ultrafiltration, DEAE cellulose-52 chromatography and Sephadex G-200 size-exclusion chromatography.
26454111	3	33	theme	polysaccharide	371:384	arg1	Abap1001					418:425	Abap1001	418:425	Abap1001	418:425	From this wastewater, four polysaccharide components (Abnp1001, Abnp1002, Abap1001, and Abap1002) with hepatic-protective activity were isolated by ultrafiltration, DEAE cellulose-52 chromatography and Sephadex G-200 size-exclusion chromatography.
26454111	3	33	theme	polysaccharide	371:384	arg1	Abap1002					432:439	Abap1002	432:439	Abap1002	432:439	From this wastewater, four polysaccharide components (Abnp1001, Abnp1002, Abap1001, and Abap1002) with hepatic-protective activity were isolated by ultrafiltration, DEAE cellulose-52 chromatography and Sephadex G-200 size-exclusion chromatography.
26454111	3	33	theme	polysaccharide	371:384	arg1	Abnp1002					408:415	Abnp1002	408:415	Abnp1002	408:415	From this wastewater, four polysaccharide components (Abnp1001, Abnp1002, Abap1001, and Abap1002) with hepatic-protective activity were isolated by ultrafiltration, DEAE cellulose-52 chromatography and Sephadex G-200 size-exclusion chromatography.
26454111	3	33	theme	polysaccharide	371:384	arg1	Abnp1001					398:405	Abnp1001	398:405	Abnp1001	398:405	From this wastewater, four polysaccharide components (Abnp1001, Abnp1002, Abap1001, and Abap1002) with hepatic-protective activity were isolated by ultrafiltration, DEAE cellulose-52 chromatography and Sephadex G-200 size-exclusion chromatography.
26454111	4	34	theme	molecular	636:644	arg1	Results					592:598	Results	592:598	Results of ultraviolet spectra analysis and molecular weight determination	592:665	Results of ultraviolet spectra analysis and molecular weight determination showed that Abnp1001, Abnp1002, Abap1001 and Abap1002 were uniform with average molecular weights of 336, 12.8, 330 and 15.8kDa, respectively.
26454111	4	34	theme	molecular	636:644	arg1	determination					653:665	molecular weight determination	636:665	molecular weight determination	636:665	Results of ultraviolet spectra analysis and molecular weight determination showed that Abnp1001, Abnp1002, Abap1001 and Abap1002 were uniform with average molecular weights of 336, 12.8, 330 and 15.8kDa, respectively.
26454111	9	35	contain	have	1709:1712	arg2	potential					1714:1722	potential	1714:1722	potential	1714:1722	These results indicate that these two polysaccharides had protective effects on acute hepatic injury induced by CCl4 in mice and suggest that the polysaccharides extracted from A. bisporus industrial wastewater might have potential in therapeutics of acute hepatic injury.
26454111	9	35	contain	have	1709:1712	arg1	polysaccharides					1638:1652	the polysaccharides	1634:1652	the polysaccharides extracted from A. bisporus industrial wastewater	1634:1701	These results indicate that these two polysaccharides had protective effects on acute hepatic injury induced by CCl4 in mice and suggest that the polysaccharides extracted from A. bisporus industrial wastewater might have potential in therapeutics of acute hepatic injury.
26454111	1	36	theme	mushroom	185:192	arg1	production					164:173	the industrial production	149:173	the industrial production of canned mushroom (Agaricus bisporus)	149:212	During the industrial production of canned mushroom (Agaricus bisporus), a large quantity of wastewater is produced.
26454111	8	37	theme	dependent	1380:1388	arg1	manner					1390:1395	a dose dependent manner	1373:1395	a dose dependent manner	1373:1395	Notably, Abnp1002 and Abap1002 could lower the alanine aminotransferase (ALT) and aspartate aminotransferase (AST) concentrations in serum in a dose dependent manner and reduce the hepatocellular degeneration and necrosis, as well as inflammatory infiltration.
26454111	8	38	theme	aminotransferase	1286:1301	arg1	concentrations					1346:1359	the alanine aminotransferase (ALT) and aspartate aminotransferase (AST) concentrations	1274:1359	the alanine aminotransferase (ALT) and aspartate aminotransferase (AST) concentrations in serum	1274:1368	Notably, Abnp1002 and Abap1002 could lower the alanine aminotransferase (ALT) and aspartate aminotransferase (AST) concentrations in serum in a dose dependent manner and reduce the hepatocellular degeneration and necrosis, as well as inflammatory infiltration.
26454111	9	39	theme	hepatic	1578:1584	arg1	injury					1586:1591	acute hepatic injury	1572:1591	acute hepatic injury induced by CCl4 in mice	1572:1615	These results indicate that these two polysaccharides had protective effects on acute hepatic injury induced by CCl4 in mice and suggest that the polysaccharides extracted from A. bisporus industrial wastewater might have potential in therapeutics of acute hepatic injury.
26454111	9	40	theme	bisporus	1672:1679	arg1	wastewater					1692:1701	A. bisporus industrial wastewater	1669:1701	A. bisporus industrial wastewater	1669:1701	These results indicate that these two polysaccharides had protective effects on acute hepatic injury induced by CCl4 in mice and suggest that the polysaccharides extracted from A. bisporus industrial wastewater might have potential in therapeutics of acute hepatic injury.
26454111	1	41	theme	large	217:221	arg1	quantity					223:230	a large quantity	215:230	a large quantity of wastewater	215:244	During the industrial production of canned mushroom (Agaricus bisporus), a large quantity of wastewater is produced.
26454111	8	42	theme	alanine	1278:1284	arg1	aminotransferase					1286:1301	alanine aminotransferase	1278:1301	alanine aminotransferase (ALT)	1278:1307	Notably, Abnp1002 and Abap1002 could lower the alanine aminotransferase (ALT) and aspartate aminotransferase (AST) concentrations in serum in a dose dependent manner and reduce the hepatocellular degeneration and necrosis, as well as inflammatory infiltration.
26454111	8	42	theme	alanine	1278:1284	arg1	ALT					1304:1306	ALT	1304:1306	ALT	1304:1306	Notably, Abnp1002 and Abap1002 could lower the alanine aminotransferase (ALT) and aspartate aminotransferase (AST) concentrations in serum in a dose dependent manner and reduce the hepatocellular degeneration and necrosis, as well as inflammatory infiltration.
26454111	6	43	theme	FT-IR	998:1002	arg1	analysis					1005:1012	Fourier transform-infrared (FT-IR) analysis	970:1012	Fourier transform-infrared (FT-IR) analysis	970:1012	Fourier transform-infrared (FT-IR) analysis showed that the isolated fractions were all composed of β-glycoside linkages.
26454111	7	44	theme	potential	1110:1118	arg1	activities					1137:1146	the potential hepatoprotective activities	1106:1146	the potential hepatoprotective activities of these polysaccharides against CCl4-induced hepatic injury in mice	1106:1215	Additionally, the potential hepatoprotective activities of these polysaccharides against CCl4-induced hepatic injury in mice were studied.
26454111	5	45	theme	gas	856:858	arg1	GC					876:877	GC	876:877	GC	876:877	The monosaccharide composition analysis using gas chromatography (GC) showed that the four fractions were heteropolysaccharides and mainly composed of glucose.
26454111	5	45	theme	gas	856:858	arg1	chromatography					860:873	gas chromatography	856:873	gas chromatography (GC)	856:878	The monosaccharide composition analysis using gas chromatography (GC) showed that the four fractions were heteropolysaccharides and mainly composed of glucose.
26454111	0	46	theme	bisporus	66:73	arg1	wastewater					86:95	Agaricus bisporus industrial wastewater	57:95	Agaricus bisporus industrial wastewater against CCl₄-induced hepatic injury in mice	57:139	Hepatoprotective effects of polysaccharide isolated from Agaricus bisporus industrial wastewater against CCl₄-induced hepatic injury in mice.
26454111	8	47	dep	concentrations	1346:1359	arg1	lower					1268:1272	lower	1268:1272	lower	1268:1272	Notably, Abnp1002 and Abap1002 could lower the alanine aminotransferase (ALT) and aspartate aminotransferase (AST) concentrations in serum in a dose dependent manner and reduce the hepatocellular degeneration and necrosis, as well as inflammatory infiltration.
26454111	0	48	theme	Agaricus	57:64	arg1	wastewater					86:95	Agaricus bisporus industrial wastewater	57:95	Agaricus bisporus industrial wastewater against CCl₄-induced hepatic injury in mice	57:139	Hepatoprotective effects of polysaccharide isolated from Agaricus bisporus industrial wastewater against CCl₄-induced hepatic injury in mice.
26454111	6	49	theme	transform-infrared	978:995	arg1	analysis					1005:1012	Fourier transform-infrared (FT-IR) analysis	970:1012	Fourier transform-infrared (FT-IR) analysis	970:1012	Fourier transform-infrared (FT-IR) analysis showed that the isolated fractions were all composed of β-glycoside linkages.
26454111	8	50	theme	inflammatory	1465:1476	arg1	infiltration					1478:1489	inflammatory infiltration	1465:1489	inflammatory infiltration	1465:1489	Notably, Abnp1002 and Abap1002 could lower the alanine aminotransferase (ALT) and aspartate aminotransferase (AST) concentrations in serum in a dose dependent manner and reduce the hepatocellular degeneration and necrosis, as well as inflammatory infiltration.
26454111	9	51	theme	injury	1757:1762	arg1	therapeutics					1727:1738	therapeutics	1727:1738	therapeutics of acute hepatic injury	1727:1762	These results indicate that these two polysaccharides had protective effects on acute hepatic injury induced by CCl4 in mice and suggest that the polysaccharides extracted from A. bisporus industrial wastewater might have potential in therapeutics of acute hepatic injury.
26454111	4	52	theme	molecular	747:755	arg1	weights					757:763	average molecular weights	739:763	average molecular weights of 336, 12.8, 330 and 15.8kDa, respectively	739:807	Results of ultraviolet spectra analysis and molecular weight determination showed that Abnp1001, Abnp1002, Abap1001 and Abap1002 were uniform with average molecular weights of 336, 12.8, 330 and 15.8kDa, respectively.
26454111	6	53	theme	Fourier	970:976	arg1	analysis					1005:1012	Fourier transform-infrared (FT-IR) analysis	970:1012	Fourier transform-infrared (FT-IR) analysis	970:1012	Fourier transform-infrared (FT-IR) analysis showed that the isolated fractions were all composed of β-glycoside linkages.
26454111	5	54	theme	monosaccharide	814:827	arg1	analysis					841:848	The monosaccharide composition analysis	810:848	The monosaccharide composition analysis using gas chromatography (GC)	810:878	The monosaccharide composition analysis using gas chromatography (GC) showed that the four fractions were heteropolysaccharides and mainly composed of glucose.
26454111	9	55	theme	industrial	1681:1690	arg1	wastewater					1692:1701	A. bisporus industrial wastewater	1669:1701	A. bisporus industrial wastewater	1669:1701	These results indicate that these two polysaccharides had protective effects on acute hepatic injury induced by CCl4 in mice and suggest that the polysaccharides extracted from A. bisporus industrial wastewater might have potential in therapeutics of acute hepatic injury.
26454111	4	56	theme	336	768:770	arg1	weights					757:763	average molecular weights	739:763	average molecular weights of 336, 12.8, 330 and 15.8kDa, respectively	739:807	Results of ultraviolet spectra analysis and molecular weight determination showed that Abnp1001, Abnp1002, Abap1001 and Abap1002 were uniform with average molecular weights of 336, 12.8, 330 and 15.8kDa, respectively.
26454111	4	57	theme	330	779:781	arg1	weights					757:763	average molecular weights	739:763	average molecular weights of 336, 12.8, 330 and 15.8kDa, respectively	739:807	Results of ultraviolet spectra analysis and molecular weight determination showed that Abnp1001, Abnp1002, Abap1001 and Abap1002 were uniform with average molecular weights of 336, 12.8, 330 and 15.8kDa, respectively.
26454111	4	58	theme	weight	646:651	arg1	Results					592:598	Results	592:598	Results of ultraviolet spectra analysis and molecular weight determination	592:665	Results of ultraviolet spectra analysis and molecular weight determination showed that Abnp1001, Abnp1002, Abap1001 and Abap1002 were uniform with average molecular weights of 336, 12.8, 330 and 15.8kDa, respectively.
26454111	4	58	theme	weight	646:651	arg1	determination					653:665	molecular weight determination	636:665	molecular weight determination	636:665	Results of ultraviolet spectra analysis and molecular weight determination showed that Abnp1001, Abnp1002, Abap1001 and Abap1002 were uniform with average molecular weights of 336, 12.8, 330 and 15.8kDa, respectively.
26454111	8	59	theme	hepatocellular	1412:1425	arg1	degeneration					1427:1438	the hepatocellular degeneration	1408:1438	the hepatocellular degeneration	1408:1438	Notably, Abnp1002 and Abap1002 could lower the alanine aminotransferase (ALT) and aspartate aminotransferase (AST) concentrations in serum in a dose dependent manner and reduce the hepatocellular degeneration and necrosis, as well as inflammatory infiltration.
26454111	0	60	from	injury	126:131	arg1	mice					136:139	mice	136:139	mice	136:139	Hepatoprotective effects of polysaccharide isolated from Agaricus bisporus industrial wastewater against CCl₄-induced hepatic injury in mice.
26454111	4	61	theme	15.8kDa	787:793	arg1	weights					757:763	average molecular weights	739:763	average molecular weights of 336, 12.8, 330 and 15.8kDa, respectively	739:807	Results of ultraviolet spectra analysis and molecular weight determination showed that Abnp1001, Abnp1002, Abap1001 and Abap1002 were uniform with average molecular weights of 336, 12.8, 330 and 15.8kDa, respectively.
26454111	7	62	theme	CCl4-induced	1181:1192	arg1	injury					1202:1207	CCl4-induced hepatic injury	1181:1207	CCl4-induced hepatic injury in mice	1181:1215	Additionally, the potential hepatoprotective activities of these polysaccharides against CCl4-induced hepatic injury in mice were studied.
26454111	2	63	theme	mushroom	321:328	arg1	canning					310:316	the canning	306:316	the canning of mushroom	306:328	In this study, the wastewater generated during the canning of mushroom was analyzed.
26454111	1	64	theme	wastewater	235:244	arg1	quantity					223:230	a large quantity	215:230	a large quantity of wastewater	215:244	During the industrial production of canned mushroom (Agaricus bisporus), a large quantity of wastewater is produced.
26454111	9	65	theme	hepatic	1749:1755	arg1	injury					1757:1762	acute hepatic injury	1743:1762	acute hepatic injury	1743:1762	These results indicate that these two polysaccharides had protective effects on acute hepatic injury induced by CCl4 in mice and suggest that the polysaccharides extracted from A. bisporus industrial wastewater might have potential in therapeutics of acute hepatic injury.
26454111	3	66	theme	cellulose-52	514:525	arg1	ultrafiltration					492:506	ultrafiltration	492:506	ultrafiltration	492:506	From this wastewater, four polysaccharide components (Abnp1001, Abnp1002, Abap1001, and Abap1002) with hepatic-protective activity were isolated by ultrafiltration, DEAE cellulose-52 chromatography and Sephadex G-200 size-exclusion chromatography.
26454111	3	66	theme	cellulose-52	514:525	arg1	chromatography					527:540	DEAE cellulose-52 chromatography	509:540	DEAE cellulose-52 chromatography	509:540	From this wastewater, four polysaccharide components (Abnp1001, Abnp1002, Abap1001, and Abap1002) with hepatic-protective activity were isolated by ultrafiltration, DEAE cellulose-52 chromatography and Sephadex G-200 size-exclusion chromatography.
26454111	0	67	theme	industrial	75:84	arg1	wastewater					86:95	Agaricus bisporus industrial wastewater	57:95	Agaricus bisporus industrial wastewater against CCl₄-induced hepatic injury in mice	57:139	Hepatoprotective effects of polysaccharide isolated from Agaricus bisporus industrial wastewater against CCl₄-induced hepatic injury in mice.
26454111	3	68	attach	isolated	480:487	arg2	Abnp1002					408:415	Abnp1002	408:415	Abnp1002	408:415	From this wastewater, four polysaccharide components (Abnp1001, Abnp1002, Abap1001, and Abap1002) with hepatic-protective activity were isolated by ultrafiltration, DEAE cellulose-52 chromatography and Sephadex G-200 size-exclusion chromatography.
26454111	3	68	attach	isolated	480:487	arg1	wastewater					354:363	this wastewater	349:363	this wastewater	349:363	From this wastewater, four polysaccharide components (Abnp1001, Abnp1002, Abap1001, and Abap1002) with hepatic-protective activity were isolated by ultrafiltration, DEAE cellulose-52 chromatography and Sephadex G-200 size-exclusion chromatography.
26454111	3	68	attach	isolated	480:487	arg2	components					386:395	four polysaccharide components	366:395	four polysaccharide components (Abnp1001, Abnp1002, Abap1001, and Abap1002) with hepatic-protective activity	366:473	From this wastewater, four polysaccharide components (Abnp1001, Abnp1002, Abap1001, and Abap1002) with hepatic-protective activity were isolated by ultrafiltration, DEAE cellulose-52 chromatography and Sephadex G-200 size-exclusion chromatography.
26454111	3	68	attach	isolated	480:487	arg2	Abap1001					418:425	Abap1001	418:425	Abap1001	418:425	From this wastewater, four polysaccharide components (Abnp1001, Abnp1002, Abap1001, and Abap1002) with hepatic-protective activity were isolated by ultrafiltration, DEAE cellulose-52 chromatography and Sephadex G-200 size-exclusion chromatography.
26454111	3	68	attach	isolated	480:487	arg2	Abap1002					432:439	Abap1002	432:439	Abap1002	432:439	From this wastewater, four polysaccharide components (Abnp1001, Abnp1002, Abap1001, and Abap1002) with hepatic-protective activity were isolated by ultrafiltration, DEAE cellulose-52 chromatography and Sephadex G-200 size-exclusion chromatography.
26454111	3	68	attach	isolated	480:487	arg2	Abnp1001					398:405	Abnp1001	398:405	Abnp1001	398:405	From this wastewater, four polysaccharide components (Abnp1001, Abnp1002, Abap1001, and Abap1002) with hepatic-protective activity were isolated by ultrafiltration, DEAE cellulose-52 chromatography and Sephadex G-200 size-exclusion chromatography.
26454111	6	69	theme	β-glycoside	1070:1080	arg1	linkages					1082:1089	β-glycoside linkages	1070:1089	β-glycoside linkages	1070:1089	Fourier transform-infrared (FT-IR) analysis showed that the isolated fractions were all composed of β-glycoside linkages.
24274499	2	0	dep	morphology	290:299	arg1	The					286:288	The	286:288	The	286:288	The morphology and average diameter of CA mats and the mats coated with LY-REC were investigated by scanning electron microscopy.
24274499	1	1	theme	rectorite	163:171	arg1	composites					179:188	positively charged lysozyme (LY) and rectorite (REC) composites	126:188	positively charged lysozyme (LY) and rectorite (REC) composites	126:188	A mixture of positively charged lysozyme (LY) and rectorite (REC) composites was electrosprayed onto negatively charged electrospun cellulose acetate (CA) nanofibrous mats.
24274499	6	2	from	fibers	1074:1079	arg1	removal					1041:1047	removal	1041:1047	removal of unbound lysozyme from fibers	1041:1079	The protein delivery properties and the bound enzyme activity after removal of unbound lysozyme from fibers were measured and showed that the electrospraying technique was suitable for enzyme immobilization.
24274499	4	3	theme	REC	779:781	arg1	exfoliated					799:808	exfoliated	799:808	exfoliated	799:808	The small-angle X-ray diffraction results showed that the silicate layers of REC were completely exfoliated.
24274499	4	3	theme	REC	779:781	arg1	layers					769:774	the silicate layers	756:774	the silicate layers of REC	756:781	The small-angle X-ray diffraction results showed that the silicate layers of REC were completely exfoliated.
24274499	4	4	theme	silicate	760:767	arg1	exfoliated					799:808	exfoliated	799:808	exfoliated	799:808	The small-angle X-ray diffraction results showed that the silicate layers of REC were completely exfoliated.
24274499	4	4	theme	silicate	760:767	arg1	layers					769:774	the silicate layers	756:774	the silicate layers of REC	756:781	The small-angle X-ray diffraction results showed that the silicate layers of REC were completely exfoliated.
24274499	1	5	theme	nanofibrous	268:278	arg1	mats					280:283	negatively charged electrospun cellulose acetate (CA) nanofibrous mats	214:283	negatively charged electrospun cellulose acetate (CA) nanofibrous mats	214:283	A mixture of positively charged lysozyme (LY) and rectorite (REC) composites was electrosprayed onto negatively charged electrospun cellulose acetate (CA) nanofibrous mats.
24274499	5	6	theme	antimicrobial	883:895	arg1	effect					897:902	the antimicrobial effect	879:902	the antimicrobial effect of the composite fibrous mats	879:932	The enzyme activity and bacterial inhibition analysis verified that the antimicrobial effect of the composite fibrous mats was enhanced with the addition of REC.
24274499	5	7	theme	REC	968:970	arg1	addition					956:963	the addition	952:963	the addition of REC	952:970	The enzyme activity and bacterial inhibition analysis verified that the antimicrobial effect of the composite fibrous mats was enhanced with the addition of REC.
24274499	2	8	theme	scanning	386:393	arg1	microscopy					404:413	scanning electron microscopy	386:413	scanning electron microscopy	386:413	The morphology and average diameter of CA mats and the mats coated with LY-REC were investigated by scanning electron microscopy.
24274499	5	9	theme	composite	911:919	arg1	mats					929:932	the composite fibrous mats	907:932	the composite fibrous mats	907:932	The enzyme activity and bacterial inhibition analysis verified that the antimicrobial effect of the composite fibrous mats was enhanced with the addition of REC.
24274499	5	10	theme	inhibition	845:854	arg1	analysis					856:863	bacterial inhibition analysis	835:863	bacterial inhibition analysis	835:863	The enzyme activity and bacterial inhibition analysis verified that the antimicrobial effect of the composite fibrous mats was enhanced with the addition of REC.
24274499	3	11	theme	composite	420:428	arg1	mats					430:433	The composite mats	416:433	The composite mats	416:433	The composite mats were characterized by Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy and energy-dispersive X-ray spectroscopy, and the results confirmed that LY and REC were successfully immobilized on the surface of CA mats via electrospraying technique.
24274499	0	12	theme	Antibacterial	0:12	arg1	activity					14:21	Antibacterial activity	0:21	Antibacterial activity of nanofibrous	0:36	Antibacterial activity of nanofibrous mats coated with lysozyme-layered silicate composites via electrospraying.
24274499	6	13	theme	enzyme	1019:1024	arg1	activity					1026:1033	the bound enzyme activity	1009:1033	the bound enzyme activity	1009:1033	The protein delivery properties and the bound enzyme activity after removal of unbound lysozyme from fibers were measured and showed that the electrospraying technique was suitable for enzyme immobilization.
24274499	1	14	theme	composites	179:188	arg1	mixture					115:121	A mixture	113:121	A mixture of positively charged lysozyme (LY) and rectorite (REC) composites	113:188	A mixture of positively charged lysozyme (LY) and rectorite (REC) composites was electrosprayed onto negatively charged electrospun cellulose acetate (CA) nanofibrous mats.
24274499	0	15	theme	nanofibrous	26:36	arg1	activity					14:21	Antibacterial activity	0:21	Antibacterial activity of nanofibrous	0:36	Antibacterial activity of nanofibrous mats coated with lysozyme-layered silicate composites via electrospraying.
24274499	6	16	theme	bound	1013:1017	arg1	activity					1026:1033	the bound enzyme activity	1009:1033	the bound enzyme activity	1009:1033	The protein delivery properties and the bound enzyme activity after removal of unbound lysozyme from fibers were measured and showed that the electrospraying technique was suitable for enzyme immobilization.
24274499	2	17	theme	average	305:311	arg1	diameter					313:320	average diameter	305:320	average diameter	305:320	The morphology and average diameter of CA mats and the mats coated with LY-REC were investigated by scanning electron microscopy.
24274499	3	18	theme	mats	666:669	arg1	surface					652:658	the surface	648:658	the surface of CA mats	648:669	The composite mats were characterized by Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy and energy-dispersive X-ray spectroscopy, and the results confirmed that LY and REC were successfully immobilized on the surface of CA mats via electrospraying technique.
24274499	4	19	theme	diffraction	724:734	arg1	results					736:742	The small-angle X-ray diffraction results	702:742	The small-angle X-ray diffraction results	702:742	The small-angle X-ray diffraction results showed that the silicate layers of REC were completely exfoliated.
24274499	4	20	theme	X-ray	718:722	arg1	diffraction					724:734	small-angle X-ray diffraction	706:734	The small-angle X-ray diffraction results	702:742	The small-angle X-ray diffraction results showed that the silicate layers of REC were completely exfoliated.
24274499	5	21	theme	enzyme	815:820	arg1	activity					822:829	The enzyme activity	811:829	The enzyme activity	811:829	The enzyme activity and bacterial inhibition analysis verified that the antimicrobial effect of the composite fibrous mats was enhanced with the addition of REC.
24274499	3	22	theme	photoelectron	504:516	arg1	spectroscopy					518:529	X-ray photoelectron spectroscopy	498:529	X-ray photoelectron spectroscopy	498:529	The composite mats were characterized by Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy and energy-dispersive X-ray spectroscopy, and the results confirmed that LY and REC were successfully immobilized on the surface of CA mats via electrospraying technique.
24274499	5	23	theme	mats	929:932	arg1	effect					897:902	the antimicrobial effect	879:902	the antimicrobial effect of the composite fibrous mats	879:932	The enzyme activity and bacterial inhibition analysis verified that the antimicrobial effect of the composite fibrous mats was enhanced with the addition of REC.
24274499	5	24	theme	fibrous	921:927	arg1	mats					929:932	the composite fibrous mats	907:932	the composite fibrous mats	907:932	The enzyme activity and bacterial inhibition analysis verified that the antimicrobial effect of the composite fibrous mats was enhanced with the addition of REC.
24274499	2	25	theme	electron	395:402	arg1	microscopy					404:413	scanning electron microscopy	386:413	scanning electron microscopy	386:413	The morphology and average diameter of CA mats and the mats coated with LY-REC were investigated by scanning electron microscopy.
24274499	0	26	theme	lysozyme-layered	55:70	arg1	composites					81:90	lysozyme-layered silicate composites	55:90	lysozyme-layered silicate composites	55:90	Antibacterial activity of nanofibrous mats coated with lysozyme-layered silicate composites via electrospraying.
24274499	6	27	theme	delivery	985:992	arg1	properties					994:1003	The protein delivery properties	973:1003	The protein delivery properties	973:1003	The protein delivery properties and the bound enzyme activity after removal of unbound lysozyme from fibers were measured and showed that the electrospraying technique was suitable for enzyme immobilization.
24274499	3	28	theme	CA	663:664	arg1	mats					666:669	CA mats	663:669	CA mats	663:669	The composite mats were characterized by Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy and energy-dispersive X-ray spectroscopy, and the results confirmed that LY and REC were successfully immobilized on the surface of CA mats via electrospraying technique.
24274499	6	29	theme	enzyme	1158:1163	arg1	immobilization					1165:1178	enzyme immobilization	1158:1178	enzyme immobilization	1158:1178	The protein delivery properties and the bound enzyme activity after removal of unbound lysozyme from fibers were measured and showed that the electrospraying technique was suitable for enzyme immobilization.
24274499	3	30	theme	X-ray	498:502	arg1	spectroscopy					518:529	X-ray photoelectron spectroscopy	498:529	X-ray photoelectron spectroscopy	498:529	The composite mats were characterized by Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy and energy-dispersive X-ray spectroscopy, and the results confirmed that LY and REC were successfully immobilized on the surface of CA mats via electrospraying technique.
24274499	5	31	theme	bacterial	835:843	arg1	analysis					856:863	bacterial inhibition analysis	835:863	bacterial inhibition analysis	835:863	The enzyme activity and bacterial inhibition analysis verified that the antimicrobial effect of the composite fibrous mats was enhanced with the addition of REC.
24274499	6	32	theme	protein	977:983	arg1	properties					994:1003	The protein delivery properties	973:1003	The protein delivery properties	973:1003	The protein delivery properties and the bound enzyme activity after removal of unbound lysozyme from fibers were measured and showed that the electrospraying technique was suitable for enzyme immobilization.
24274499	0	33	dep	mats	38:41	arg1	coated					43:48	coated	43:48	mats coated with lysozyme-layered silicate composites via electrospraying	38:110	Antibacterial activity of nanofibrous mats coated with lysozyme-layered silicate composites via electrospraying.
24274499	1	34	theme	charged	137:143	arg1	lysozyme					145:152	positively charged lysozyme	126:152	positively charged lysozyme (LY)	126:157	A mixture of positively charged lysozyme (LY) and rectorite (REC) composites was electrosprayed onto negatively charged electrospun cellulose acetate (CA) nanofibrous mats.
24274499	1	34	theme	charged	137:143	arg1	LY					155:156	LY	155:156	LY	155:156	A mixture of positively charged lysozyme (LY) and rectorite (REC) composites was electrosprayed onto negatively charged electrospun cellulose acetate (CA) nanofibrous mats.
24274499	4	35	theme	small-angle	706:716	arg1	diffraction					724:734	small-angle X-ray diffraction	706:734	The small-angle X-ray diffraction results	702:742	The small-angle X-ray diffraction results showed that the silicate layers of REC were completely exfoliated.
24274499	1	36	theme	lysozyme	145:152	arg1	composites					179:188	positively charged lysozyme (LY) and rectorite (REC) composites	126:188	positively charged lysozyme (LY) and rectorite (REC) composites	126:188	A mixture of positively charged lysozyme (LY) and rectorite (REC) composites was electrosprayed onto negatively charged electrospun cellulose acetate (CA) nanofibrous mats.
24274499	1	37	theme	charged	225:231	arg1	mats					280:283	negatively charged electrospun cellulose acetate (CA) nanofibrous mats	214:283	negatively charged electrospun cellulose acetate (CA) nanofibrous mats	214:283	A mixture of positively charged lysozyme (LY) and rectorite (REC) composites was electrosprayed onto negatively charged electrospun cellulose acetate (CA) nanofibrous mats.
24274499	3	38	dep	Fourier	457:463	arg1	transform					465:473	transform	465:473	transform infrared spectroscopy, X-ray photoelectron spectroscopy and energy-dispersive X-ray spectroscopy	465:570	The composite mats were characterized by Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy and energy-dispersive X-ray spectroscopy, and the results confirmed that LY and REC were successfully immobilized on the surface of CA mats via electrospraying technique.
24274499	2	39	theme	CA	325:326	arg1	mats					328:331	CA mats	325:331	CA mats	325:331	The morphology and average diameter of CA mats and the mats coated with LY-REC were investigated by scanning electron microscopy.
24274499	2	40	theme	mats	328:331	arg1	morphology					290:299	morphology	290:299	morphology	290:299	The morphology and average diameter of CA mats and the mats coated with LY-REC were investigated by scanning electron microscopy.
24274499	2	40	theme	mats	328:331	arg1	diameter					313:320	average diameter	305:320	average diameter	305:320	The morphology and average diameter of CA mats and the mats coated with LY-REC were investigated by scanning electron microscopy.
24274499	2	40	theme	mats	328:331	arg1	mats					341:344	the mats	337:344	the mats coated with LY-REC	337:363	The morphology and average diameter of CA mats and the mats coated with LY-REC were investigated by scanning electron microscopy.
24274499	3	41	dep	transform	465:473	arg1	infrared					475:482	infrared	475:482	transform infrared spectroscopy, X-ray photoelectron spectroscopy and energy-dispersive X-ray spectroscopy	465:570	The composite mats were characterized by Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy and energy-dispersive X-ray spectroscopy, and the results confirmed that LY and REC were successfully immobilized on the surface of CA mats via electrospraying technique.
24274499	3	42	theme	energy-dispersive	535:551	arg1	spectroscopy					559:570	energy-dispersive X-ray spectroscopy	535:570	energy-dispersive X-ray spectroscopy	535:570	The composite mats were characterized by Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy and energy-dispersive X-ray spectroscopy, and the results confirmed that LY and REC were successfully immobilized on the surface of CA mats via electrospraying technique.
24274499	6	43	theme	electrospraying	1115:1129	arg1	suitable					1145:1152	suitable	1145:1152	suitable	1145:1152	The protein delivery properties and the bound enzyme activity after removal of unbound lysozyme from fibers were measured and showed that the electrospraying technique was suitable for enzyme immobilization.
24274499	6	43	theme	electrospraying	1115:1129	arg1	technique					1131:1139	the electrospraying technique	1111:1139	the electrospraying technique	1111:1139	The protein delivery properties and the bound enzyme activity after removal of unbound lysozyme from fibers were measured and showed that the electrospraying technique was suitable for enzyme immobilization.
24274499	6	44	theme	lysozyme	1060:1067	arg1	removal					1041:1047	removal	1041:1047	removal of unbound lysozyme from fibers	1041:1079	The protein delivery properties and the bound enzyme activity after removal of unbound lysozyme from fibers were measured and showed that the electrospraying technique was suitable for enzyme immobilization.
24274499	0	45	theme	silicate	72:79	arg1	composites					81:90	lysozyme-layered silicate composites	55:90	lysozyme-layered silicate composites	55:90	Antibacterial activity of nanofibrous mats coated with lysozyme-layered silicate composites via electrospraying.
24274499	3	46	theme	X-ray	553:557	arg1	spectroscopy					559:570	energy-dispersive X-ray spectroscopy	535:570	energy-dispersive X-ray spectroscopy	535:570	The composite mats were characterized by Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy and energy-dispersive X-ray spectroscopy, and the results confirmed that LY and REC were successfully immobilized on the surface of CA mats via electrospraying technique.
24274499	6	47	theme	unbound	1052:1058	arg1	lysozyme					1060:1067	unbound lysozyme	1052:1067	unbound lysozyme	1052:1067	The protein delivery properties and the bound enzyme activity after removal of unbound lysozyme from fibers were measured and showed that the electrospraying technique was suitable for enzyme immobilization.
28341173	11	0	theme	contact	1453:1459	arg1	surface					1461:1467	a large contact surface	1445:1467	a large contact surface	1445:1467	This would be due to the high aspect ratio of BC that is capable of connecting between the cellulosic fibers and BC nanofibers, enhancing a large contact surface and therefore producing excellent coherence.
28341173	10	1	theme	bonding	1275:1281	arg1	increase					1248:1255	the increase	1244:1255	the increase of interfibrillar bonding which reduced porosity	1244:1304	The reason for the improved mechanical properties can be attributed to the increase of interfibrillar bonding which reduced porosity.
28341173	11	2	theme	BC	1420:1421	arg1	nanofibers					1423:1432	BC nanofibers	1420:1432	BC nanofibers	1420:1432	This would be due to the high aspect ratio of BC that is capable of connecting between the cellulosic fibers and BC nanofibers, enhancing a large contact surface and therefore producing excellent coherence.
28341173	11	3	theme	aspect	1337:1342	arg1	capable					1364:1370	capable	1364:1370	capable	1364:1370	This would be due to the high aspect ratio of BC that is capable of connecting between the cellulosic fibers and BC nanofibers, enhancing a large contact surface and therefore producing excellent coherence.
28341173	11	3	theme	aspect	1337:1342	arg1	ratio					1344:1348	the high aspect ratio	1328:1348	the high aspect ratio of BC that is capable of connecting between the cellulosic fibers and BC nanofibers, enhancing a large contact surface and therefore producing excellent coherence	1328:1511	This would be due to the high aspect ratio of BC that is capable of connecting between the cellulosic fibers and BC nanofibers, enhancing a large contact surface and therefore producing excellent coherence.
28341173	4	4	theme	BC	477:478	arg1	specimens					464:472	The specimens	460:472	The specimens of BC, SP and the reinforced SP with BC	460:512	The specimens of BC, SP and the reinforced SP with BC were characterized using X-ray diffraction (XRD), FT-IR, FESEM, and physico-mechanical testing.
28341173	10	5	theme	interfibrillar	1260:1273	arg1	bonding					1275:1281	interfibrillar bonding	1260:1281	interfibrillar bonding which reduced porosity	1260:1304	The reason for the improved mechanical properties can be attributed to the increase of interfibrillar bonding which reduced porosity.
28341173	11	6	theme	BC	1353:1354	arg1	capable					1364:1370	capable	1364:1370	capable	1364:1370	This would be due to the high aspect ratio of BC that is capable of connecting between the cellulosic fibers and BC nanofibers, enhancing a large contact surface and therefore producing excellent coherence.
28341173	11	6	theme	BC	1353:1354	arg1	ratio					1344:1348	the high aspect ratio	1328:1348	the high aspect ratio of BC that is capable of connecting between the cellulosic fibers and BC nanofibers, enhancing a large contact surface and therefore producing excellent coherence	1328:1511	This would be due to the high aspect ratio of BC that is capable of connecting between the cellulosic fibers and BC nanofibers, enhancing a large contact surface and therefore producing excellent coherence.
28341173	9	7	theme	tear	1078:1081	arg1	index					1083:1087	tear index	1078:1087	tear index	1078:1087	The physical and mechanical properties showed that as the dosage of BC increased, the properties of tensile index, tear index, and burst index greatly improved, while the porosity and the elongation decreased.
28341173	7	8	with	combination	865:875	arg1	structure					894:902	a nonporous structure	882:902	a nonporous structure	882:902	Based on FESEM images, one can conclude that BC and softwood fibers do form a good combination with a nonporous structure.
28341173	8	9	theme	BC	905:906	arg1	fibers					908:913	BC fibers	905:913	BC fibers	905:913	BC fibers fill in among the softwood fibers in the sheet.
28341173	7	10	theme	softwood	834:841	arg1	fibers					843:848	BC and softwood fibers	827:848	BC and softwood fibers	827:848	Based on FESEM images, one can conclude that BC and softwood fibers do form a good combination with a nonporous structure.
28341173	4	11	theme	X-ray	539:543	arg1	XRD					558:560	XRD	558:560	XRD	558:560	The specimens of BC, SP and the reinforced SP with BC were characterized using X-ray diffraction (XRD), FT-IR, FESEM, and physico-mechanical testing.
28341173	4	11	theme	X-ray	539:543	arg1	diffraction					545:555	X-ray diffraction	539:555	X-ray diffraction (XRD)	539:561	The specimens of BC, SP and the reinforced SP with BC were characterized using X-ray diffraction (XRD), FT-IR, FESEM, and physico-mechanical testing.
28341173	2	12	theme	interfacial	276:286	arg1	adhesion					288:295	interfacial adhesion	276:295	interfacial adhesion between the cellulosic fibers and BC	276:332	BC is a nanocellulose, which was anticipated to increase interfacial adhesion between the cellulosic fibers and BC.
28341173	1	13	with	pulp	139:142	arg1	BC					175:176	BC	175:176	BC	175:176	The main goal of this work was to reinforce softwood pulp (SP) with bacterial cellulose (BC) to generate a sustainable biocomposite.
28341173	1	13	with	pulp	139:142	arg1	cellulose					164:172	bacterial cellulose	154:172	bacterial cellulose (BC)	154:177	The main goal of this work was to reinforce softwood pulp (SP) with bacterial cellulose (BC) to generate a sustainable biocomposite.
28341173	9	14	theme	BC	1031:1032	arg1	dosage					1021:1026	the dosage	1017:1026	the dosage of BC	1017:1032	The physical and mechanical properties showed that as the dosage of BC increased, the properties of tensile index, tear index, and burst index greatly improved, while the porosity and the elongation decreased.
28341173	2	15	theme	cellulosic	309:318	arg1	fibers					320:325	the cellulosic fibers	305:325	the cellulosic fibers	305:325	BC is a nanocellulose, which was anticipated to increase interfacial adhesion between the cellulosic fibers and BC.
28341173	7	16	theme	BC	827:828	arg1	fibers					843:848	BC and softwood fibers	827:848	BC and softwood fibers	827:848	Based on FESEM images, one can conclude that BC and softwood fibers do form a good combination with a nonporous structure.
28341173	4	17	with	SP	503:504	arg1	BC					511:512	BC	511:512	BC	511:512	The specimens of BC, SP and the reinforced SP with BC were characterized using X-ray diffraction (XRD), FT-IR, FESEM, and physico-mechanical testing.
28341173	6	18	theme	SP	771:772	arg1	fibers					774:779	SP fibers	771:779	SP fibers	771:779	FT-IR spectra showed that the composition of BC was fully different from that of SP fibers.
28341173	3	19	dep	xylinus	375:381	arg1	incubated					394:402	incubated	394:402	was incubated in a static Hestrin-Schramm culture at 28°C for 14days	390:457	The organism used was Gluconacetobacter xylinus, which was incubated in a static Hestrin-Schramm culture at 28°C for 14days.
28341173	6	20	theme	FT-IR	690:694	arg1	spectra					696:702	FT-IR spectra	690:702	FT-IR spectra	690:702	FT-IR spectra showed that the composition of BC was fully different from that of SP fibers.
28341173	1	21	theme	main	90:93	arg1	goal					95:98	The main goal	86:98	The main goal of this work	86:111	The main goal of this work was to reinforce softwood pulp (SP) with bacterial cellulose (BC) to generate a sustainable biocomposite.
28341173	1	22	theme	bacterial	154:162	arg1	BC					175:176	BC	175:176	BC	175:176	The main goal of this work was to reinforce softwood pulp (SP) with bacterial cellulose (BC) to generate a sustainable biocomposite.
28341173	1	22	theme	bacterial	154:162	arg1	cellulose					164:172	bacterial cellulose	154:172	bacterial cellulose (BC)	154:177	The main goal of this work was to reinforce softwood pulp (SP) with bacterial cellulose (BC) to generate a sustainable biocomposite.
28341173	4	23	theme	SP	503:504	arg1	specimens					464:472	The specimens	460:472	The specimens of BC, SP and the reinforced SP with BC	460:512	The specimens of BC, SP and the reinforced SP with BC were characterized using X-ray diffraction (XRD), FT-IR, FESEM, and physico-mechanical testing.
28341173	12	24	theme	promising	1553:1561	arg1	material					1563:1570	a promising material	1551:1570	a promising material for reinforcing composites at low loading	1551:1612	This study suggests that BC could be a promising material for reinforcing composites at low loading.
28341173	12	24	theme	promising	1553:1561	arg1	BC					1539:1540	BC	1539:1540	BC	1539:1540	This study suggests that BC could be a promising material for reinforcing composites at low loading.
28341173	4	25	theme	physico-mechanical	582:599	arg1	testing					601:607	physico-mechanical testing	582:607	physico-mechanical testing	582:607	The specimens of BC, SP and the reinforced SP with BC were characterized using X-ray diffraction (XRD), FT-IR, FESEM, and physico-mechanical testing.
28341173	4	26	with	BC	477:478	arg1	BC					511:512	BC	511:512	BC	511:512	The specimens of BC, SP and the reinforced SP with BC were characterized using X-ray diffraction (XRD), FT-IR, FESEM, and physico-mechanical testing.
28341173	4	27	theme	reinforced	492:501	arg1	SP					503:504	the reinforced SP	488:504	the reinforced SP	488:504	The specimens of BC, SP and the reinforced SP with BC were characterized using X-ray diffraction (XRD), FT-IR, FESEM, and physico-mechanical testing.
28341173	8	28	theme	softwood	933:940	arg1	fibers					942:947	the softwood fibers	929:947	the softwood fibers in the sheet	929:960	BC fibers fill in among the softwood fibers in the sheet.
28341173	10	29	theme	improved	1192:1199	arg1	properties					1212:1221	the improved mechanical properties	1188:1221	the improved mechanical properties	1188:1221	The reason for the improved mechanical properties can be attributed to the increase of interfibrillar bonding which reduced porosity.
28341173	4	30	with	SP	481:482	arg1	BC					511:512	BC	511:512	BC	511:512	The specimens of BC, SP and the reinforced SP with BC were characterized using X-ray diffraction (XRD), FT-IR, FESEM, and physico-mechanical testing.
28341173	9	31	theme	mechanical	980:989	arg1	properties					991:1000	The physical and mechanical properties	963:1000	The physical and mechanical properties	963:1000	The physical and mechanical properties showed that as the dosage of BC increased, the properties of tensile index, tear index, and burst index greatly improved, while the porosity and the elongation decreased.
28341173	9	32	theme	tensile	1063:1069	arg1	index					1071:1075	tensile index	1063:1075	tensile index	1063:1075	The physical and mechanical properties showed that as the dosage of BC increased, the properties of tensile index, tear index, and burst index greatly improved, while the porosity and the elongation decreased.
28341173	0	33	theme	papers	47:52	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of reinforced papers using nano bacterial cellulose.
28341173	0	33	theme	papers	47:52	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of reinforced papers using nano bacterial cellulose.
28341173	9	34	theme	index	1071:1075	arg1	properties					1049:1058	the properties	1045:1058	the properties of tensile index, tear index, and burst index	1045:1104	The physical and mechanical properties showed that as the dosage of BC increased, the properties of tensile index, tear index, and burst index greatly improved, while the porosity and the elongation decreased.
28341173	3	35	theme	static	409:414	arg1	culture					432:438	a static Hestrin-Schramm culture	407:438	a static Hestrin-Schramm culture	407:438	The organism used was Gluconacetobacter xylinus, which was incubated in a static Hestrin-Schramm culture at 28°C for 14days.
28341173	0	36	theme	reinforced	36:45	arg1	papers					47:52	reinforced papers	36:52	reinforced papers	36:52	Preparation and characterization of reinforced papers using nano bacterial cellulose.
28341173	4	37	theme	SP	481:482	arg1	specimens					464:472	The specimens	460:472	The specimens of BC, SP and the reinforced SP with BC	460:512	The specimens of BC, SP and the reinforced SP with BC were characterized using X-ray diffraction (XRD), FT-IR, FESEM, and physico-mechanical testing.
28341173	11	38	theme	excellent	1493:1501	arg1	coherence					1503:1511	excellent coherence	1493:1511	excellent coherence	1493:1511	This would be due to the high aspect ratio of BC that is capable of connecting between the cellulosic fibers and BC nanofibers, enhancing a large contact surface and therefore producing excellent coherence.
28341173	6	39	from	that	763:766	arg1	different					748:756	different	748:756	different	748:756	FT-IR spectra showed that the composition of BC was fully different from that of SP fibers.
28341173	6	39	from	that	763:766	arg1	composition					720:730	the composition	716:730	the composition of BC	716:736	FT-IR spectra showed that the composition of BC was fully different from that of SP fibers.
28341173	9	40	theme	physical	967:974	arg1	properties					991:1000	The physical and mechanical properties	963:1000	The physical and mechanical properties	963:1000	The physical and mechanical properties showed that as the dosage of BC increased, the properties of tensile index, tear index, and burst index greatly improved, while the porosity and the elongation decreased.
28341173	3	41	theme	Hestrin-Schramm	416:430	arg1	culture					432:438	a static Hestrin-Schramm culture	407:438	a static Hestrin-Schramm culture	407:438	The organism used was Gluconacetobacter xylinus, which was incubated in a static Hestrin-Schramm culture at 28°C for 14days.
28341173	3	42	theme	Gluconacetobacter	357:373	arg1	xylinus					375:381	Gluconacetobacter xylinus	357:381	Gluconacetobacter xylinus	357:381	The organism used was Gluconacetobacter xylinus, which was incubated in a static Hestrin-Schramm culture at 28°C for 14days.
28341173	3	42	theme	Gluconacetobacter	357:373	arg1	organism					339:346	The organism	335:346	The organism used	335:351	The organism used was Gluconacetobacter xylinus, which was incubated in a static Hestrin-Schramm culture at 28°C for 14days.
28341173	0	43	theme	bacterial	65:73	arg1	cellulose					75:83	nano bacterial cellulose	60:83	nano bacterial cellulose	60:83	Preparation and characterization of reinforced papers using nano bacterial cellulose.
28341173	7	44	theme	nonporous	884:892	arg1	structure					894:902	a nonporous structure	882:902	a nonporous structure	882:902	Based on FESEM images, one can conclude that BC and softwood fibers do form a good combination with a nonporous structure.
28341173	1	45	theme	work	108:111	arg1	goal					95:98	The main goal	86:98	The main goal of this work	86:111	The main goal of this work was to reinforce softwood pulp (SP) with bacterial cellulose (BC) to generate a sustainable biocomposite.
28341173	11	46	theme	large	1447:1451	arg1	surface					1461:1467	a large contact surface	1445:1467	a large contact surface	1445:1467	This would be due to the high aspect ratio of BC that is capable of connecting between the cellulosic fibers and BC nanofibers, enhancing a large contact surface and therefore producing excellent coherence.
28341173	9	47	theme	index	1083:1087	arg1	properties					1049:1058	the properties	1045:1058	the properties of tensile index, tear index, and burst index	1045:1104	The physical and mechanical properties showed that as the dosage of BC increased, the properties of tensile index, tear index, and burst index greatly improved, while the porosity and the elongation decreased.
28341173	7	48	theme	good	860:863	arg1	combination					865:875	a good combination	858:875	a good combination with a nonporous structure	858:902	Based on FESEM images, one can conclude that BC and softwood fibers do form a good combination with a nonporous structure.
28341173	7	49	theme	FESEM	791:795	arg1	images					797:802	FESEM images	791:802	FESEM images	791:802	Based on FESEM images, one can conclude that BC and softwood fibers do form a good combination with a nonporous structure.
28341173	5	50	theme	crystallinity	614:626	arg1	index					628:632	The crystallinity index	610:632	The crystallinity index	610:632	The crystallinity index was found to be 83 and 54% for BC and SP, respectively.
28341173	5	50	theme	crystallinity	614:626	arg1	%					659:659	83 and 54%	650:659	83 and 54% for BC and SP	650:673	The crystallinity index was found to be 83 and 54% for BC and SP, respectively.
28341173	6	51	theme	BC	735:736	arg1	different					748:756	different	748:756	different	748:756	FT-IR spectra showed that the composition of BC was fully different from that of SP fibers.
28341173	6	51	theme	BC	735:736	arg1	composition					720:730	the composition	716:730	the composition of BC	716:736	FT-IR spectra showed that the composition of BC was fully different from that of SP fibers.
28341173	10	52	theme	mechanical	1201:1210	arg1	properties					1212:1221	the improved mechanical properties	1188:1221	the improved mechanical properties	1188:1221	The reason for the improved mechanical properties can be attributed to the increase of interfibrillar bonding which reduced porosity.
28341173	11	53	theme	cellulosic	1398:1407	arg1	fibers					1409:1414	the cellulosic fibers	1394:1414	the cellulosic fibers	1394:1414	This would be due to the high aspect ratio of BC that is capable of connecting between the cellulosic fibers and BC nanofibers, enhancing a large contact surface and therefore producing excellent coherence.
28341173	12	54	theme	low	1602:1604	arg1	loading					1606:1612	low loading	1602:1612	low loading	1602:1612	This study suggests that BC could be a promising material for reinforcing composites at low loading.
28341173	8	55	from	fibers	942:947	arg1	sheet					956:960	the sheet	952:960	the sheet	952:960	BC fibers fill in among the softwood fibers in the sheet.
28341173	9	56	theme	burst	1094:1098	arg1	index					1100:1104	burst index	1094:1104	burst index	1094:1104	The physical and mechanical properties showed that as the dosage of BC increased, the properties of tensile index, tear index, and burst index greatly improved, while the porosity and the elongation decreased.
28341173	1	57	theme	softwood	130:137	arg1	pulp					139:142	softwood pulp	130:142	softwood pulp (SP) with bacterial cellulose (BC)	130:177	The main goal of this work was to reinforce softwood pulp (SP) with bacterial cellulose (BC) to generate a sustainable biocomposite.
28341173	1	57	theme	softwood	130:137	arg1	SP					145:146	SP	145:146	SP	145:146	The main goal of this work was to reinforce softwood pulp (SP) with bacterial cellulose (BC) to generate a sustainable biocomposite.
28341173	1	58	theme	sustainable	193:203	arg1	biocomposite					205:216	a sustainable biocomposite	191:216	a sustainable biocomposite	191:216	The main goal of this work was to reinforce softwood pulp (SP) with bacterial cellulose (BC) to generate a sustainable biocomposite.
28341173	0	59	theme	nano	60:63	arg1	cellulose					75:83	nano bacterial cellulose	60:83	nano bacterial cellulose	60:83	Preparation and characterization of reinforced papers using nano bacterial cellulose.
28341173	9	60	theme	index	1100:1104	arg1	properties					1049:1058	the properties	1045:1058	the properties of tensile index, tear index, and burst index	1045:1104	The physical and mechanical properties showed that as the dosage of BC increased, the properties of tensile index, tear index, and burst index greatly improved, while the porosity and the elongation decreased.
28341173	11	61	theme	high	1332:1335	arg1	capable					1364:1370	capable	1364:1370	capable	1364:1370	This would be due to the high aspect ratio of BC that is capable of connecting between the cellulosic fibers and BC nanofibers, enhancing a large contact surface and therefore producing excellent coherence.
28341173	11	61	theme	high	1332:1335	arg1	ratio					1344:1348	the high aspect ratio	1328:1348	the high aspect ratio of BC that is capable of connecting between the cellulosic fibers and BC nanofibers, enhancing a large contact surface and therefore producing excellent coherence	1328:1511	This would be due to the high aspect ratio of BC that is capable of connecting between the cellulosic fibers and BC nanofibers, enhancing a large contact surface and therefore producing excellent coherence.
24551241	6	0	theme	extracellular	965:977	arg1	components					986:995	extracellular matrix components	965:995	extracellular matrix components of the Salmonella biofilm on the surface of human cholesterol gallstones	965:1068	In this work we describe a method for sectioning and direct visualization of extracellular matrix components of the Salmonella biofilm on the surface of human cholesterol gallstones and provide a description of the major matrix components observed therein.
24551241	2	1	theme	protective	356:365	arg1	matrix					382:387	a protective macromolecular matrix	354:387	a protective macromolecular matrix	354:387	Biofilms, by definition, involve the formation of a bacterial community encased within a protective macromolecular matrix.
24551241	3	2	theme	environmental	516:528	arg1	conditions					530:539	altered environmental conditions	508:539	altered environmental conditions	508:539	Previous work has demonstrated the composition of the biofilm matrix to be complex and highly variable in response to altered environmental conditions.
24551241	11	3	theme	matrix	1784:1789	arg1	components					1791:1800	individual matrix components	1773:1800	individual matrix components	1773:1800	A better understanding of the contribution of individual matrix components to the overall biofilm structure will facilitate the development of more effective and specific methods to disrupt these bacterial communities.
24551241	7	4	theme	robust	1175:1180	arg1	formation					1190:1198	robust biofilm formation	1175:1198	robust biofilm formation	1175:1198	Confocal micrographs revealed robust biofilm formation, characterized by abundant but highly heterogeneous expression of polysaccharides such as LPS, Vi and O-antigen capsule.
24551241	4	5	theme	physiological	702:714	arg1	conditions					716:725	physiological conditions	702:725	physiological conditions	702:725	Although known to play an important role in bacterial persistence in a variety of contexts, the Salmonella biofilm matrix remains largely uncharacterized under physiological conditions.
24551241	10	6	theme	biofilm	1718:1724	arg1	architecture					1688:1699	the native architecture	1677:1699	the native architecture of the gallstone biofilm	1677:1724	These results demonstrate the use and feasibility of this method while highlighting the importance of studying the native architecture of the gallstone biofilm.
24551241	4	7	theme	biofilm	649:655	arg1	matrix					657:662	the Salmonella biofilm matrix	634:662	the Salmonella biofilm matrix	634:662	Although known to play an important role in bacterial persistence in a variety of contexts, the Salmonella biofilm matrix remains largely uncharacterized under physiological conditions.
24551241	11	8	theme	bacterial	1923:1931	arg1	communities					1933:1943	these bacterial communities	1917:1943	these bacterial communities	1917:1943	A better understanding of the contribution of individual matrix components to the overall biofilm structure will facilitate the development of more effective and specific methods to disrupt these bacterial communities.
24551241	1	9	theme	biofilms	216:223	arg1	formation					193:201	the formation	189:201	the formation of bacterial biofilms on the surface of cholesterol gallstones	189:264	Chronic carriage of Salmonella Typhi is mediated primarily through the formation of bacterial biofilms on the surface of cholesterol gallstones.
24551241	6	10	theme	biofilm	1015:1021	arg1	components					986:995	extracellular matrix components	965:995	extracellular matrix components of the Salmonella biofilm on the surface of human cholesterol gallstones	965:1068	In this work we describe a method for sectioning and direct visualization of extracellular matrix components of the Salmonella biofilm on the surface of human cholesterol gallstones and provide a description of the major matrix components observed therein.
24551241	8	11	theme	biofilm	1350:1356	arg1	matrix					1358:1363	the biofilm matrix	1346:1363	the biofilm matrix	1346:1363	CsgA was not observed in the biofilm matrix and flagellar expression was tightly restricted to the biofilm-cholesterol interface.
24551241	7	12	theme	heterogeneous	1238:1250	arg1	expression					1252:1261	abundant but highly heterogeneous expression	1218:1261	abundant but highly heterogeneous expression of polysaccharides such as LPS, Vi and O-antigen capsule	1218:1318	Confocal micrographs revealed robust biofilm formation, characterized by abundant but highly heterogeneous expression of polysaccharides such as LPS, Vi and O-antigen capsule.
24551241	0	13	from	biofilms	77:84	arg1	surface					93:99	the surface	89:99	the surface of human gallstones	89:119	Visualization of extracellular matrix components within sectioned Salmonella biofilms on the surface of human gallstones.
24551241	6	14	theme	components	986:995	arg1	sectioning					926:935	sectioning	926:935	sectioning	926:935	In this work we describe a method for sectioning and direct visualization of extracellular matrix components of the Salmonella biofilm on the surface of human cholesterol gallstones and provide a description of the major matrix components observed therein.
24551241	6	14	theme	components	986:995	arg1	visualization					948:960	direct visualization	941:960	direct visualization	941:960	In this work we describe a method for sectioning and direct visualization of extracellular matrix components of the Salmonella biofilm on the surface of human cholesterol gallstones and provide a description of the major matrix components observed therein.
24551241	1	15	from	formation	193:201	arg1	surface					232:238	the surface	228:238	the surface of cholesterol gallstones	228:264	Chronic carriage of Salmonella Typhi is mediated primarily through the formation of bacterial biofilms on the surface of cholesterol gallstones.
24551241	1	16	theme	Salmonella	142:151	arg1	Typhi					153:157	Salmonella Typhi	142:157	Salmonella Typhi	142:157	Chronic carriage of Salmonella Typhi is mediated primarily through the formation of bacterial biofilms on the surface of cholesterol gallstones.
24551241	5	17	theme	matrix	804:809	arg1	architecture					776:787	architecture	776:787	architecture of the biofilm matrix on gallstone surfaces	776:831	Initial attempts to study matrix components and architecture of the biofilm matrix on gallstone surfaces were hindered by the auto-fluorescence of cholesterol.
24551241	5	18	theme	cholesterol	875:885	arg1	auto-fluorescence					854:870	the auto-fluorescence	850:870	the auto-fluorescence of cholesterol	850:885	Initial attempts to study matrix components and architecture of the biofilm matrix on gallstone surfaces were hindered by the auto-fluorescence of cholesterol.
24551241	5	19	theme	gallstone	814:822	arg1	surfaces					824:831	gallstone surfaces	814:831	gallstone surfaces	814:831	Initial attempts to study matrix components and architecture of the biofilm matrix on gallstone surfaces were hindered by the auto-fluorescence of cholesterol.
24551241	5	20	from	surfaces	824:831	arg1	architecture					776:787	architecture	776:787	architecture of the biofilm matrix on gallstone surfaces	776:831	Initial attempts to study matrix components and architecture of the biofilm matrix on gallstone surfaces were hindered by the auto-fluorescence of cholesterol.
24551241	6	21	theme	cholesterol	1047:1057	arg1	gallstones					1059:1068	human cholesterol gallstones	1041:1068	human cholesterol gallstones	1041:1068	In this work we describe a method for sectioning and direct visualization of extracellular matrix components of the Salmonella biofilm on the surface of human cholesterol gallstones and provide a description of the major matrix components observed therein.
24551241	8	22	theme	biofilm-cholesterol	1420:1438	arg1	interface					1440:1448	the biofilm-cholesterol interface	1416:1448	the biofilm-cholesterol interface	1416:1448	CsgA was not observed in the biofilm matrix and flagellar expression was tightly restricted to the biofilm-cholesterol interface.
24551241	11	23	theme	methods	1898:1904	arg1	development					1855:1865	the development	1851:1865	the development of more effective and specific methods to disrupt these bacterial communities	1851:1943	A better understanding of the contribution of individual matrix components to the overall biofilm structure will facilitate the development of more effective and specific methods to disrupt these bacterial communities.
24551241	10	24	dep	use	1596:1598	arg1	the					1592:1594	the	1592:1594	the	1592:1594	These results demonstrate the use and feasibility of this method while highlighting the importance of studying the native architecture of the gallstone biofilm.
24551241	0	25	theme	gallstones	110:119	arg1	surface					93:99	the surface	89:99	the surface of human gallstones	89:119	Visualization of extracellular matrix components within sectioned Salmonella biofilms on the surface of human gallstones.
24551241	5	26	theme	biofilm	796:802	arg1	matrix					804:809	the biofilm matrix	792:809	the biofilm matrix on gallstone surfaces	792:831	Initial attempts to study matrix components and architecture of the biofilm matrix on gallstone surfaces were hindered by the auto-fluorescence of cholesterol.
24551241	3	27	theme	biofilm	444:450	arg1	matrix					452:457	the biofilm matrix	440:457	the biofilm matrix	440:457	Previous work has demonstrated the composition of the biofilm matrix to be complex and highly variable in response to altered environmental conditions.
24551241	7	28	theme	polysaccharides	1266:1280	arg1	expression					1252:1261	abundant but highly heterogeneous expression	1218:1261	abundant but highly heterogeneous expression of polysaccharides such as LPS, Vi and O-antigen capsule	1218:1318	Confocal micrographs revealed robust biofilm formation, characterized by abundant but highly heterogeneous expression of polysaccharides such as LPS, Vi and O-antigen capsule.
24551241	6	29	theme	components	1116:1125	arg1	description					1084:1094	a description	1082:1094	a description of the major matrix components observed therein	1082:1142	In this work we describe a method for sectioning and direct visualization of extracellular matrix components of the Salmonella biofilm on the surface of human cholesterol gallstones and provide a description of the major matrix components observed therein.
24551241	3	30	theme	Previous	390:397	arg1	work					399:402	Previous work	390:402	Previous work	390:402	Previous work has demonstrated the composition of the biofilm matrix to be complex and highly variable in response to altered environmental conditions.
24551241	4	31	theme	important	568:576	arg1	role					578:581	an important role	565:581	an important role	565:581	Although known to play an important role in bacterial persistence in a variety of contexts, the Salmonella biofilm matrix remains largely uncharacterized under physiological conditions.
24551241	0	32	theme	extracellular	17:29	arg1	components					38:47	extracellular matrix components	17:47	extracellular matrix components	17:47	Visualization of extracellular matrix components within sectioned Salmonella biofilms on the surface of human gallstones.
24551241	6	33	theme	major	1103:1107	arg1	components					1116:1125	the major matrix components	1099:1125	the major matrix components observed therein	1099:1142	In this work we describe a method for sectioning and direct visualization of extracellular matrix components of the Salmonella biofilm on the surface of human cholesterol gallstones and provide a description of the major matrix components observed therein.
24551241	6	34	from	surface	1030:1036	arg1	components					986:995	extracellular matrix components	965:995	extracellular matrix components of the Salmonella biofilm on the surface of human cholesterol gallstones	965:1068	In this work we describe a method for sectioning and direct visualization of extracellular matrix components of the Salmonella biofilm on the surface of human cholesterol gallstones and provide a description of the major matrix components observed therein.
24551241	0	35	theme	components	38:47	arg1	Visualization					0:12	Visualization	0:12	Visualization of extracellular matrix components within sectioned Salmonella biofilms on the surface of human gallstones.	0:120	Visualization of extracellular matrix components within sectioned Salmonella biofilms on the surface of human gallstones.
24551241	11	36	theme	biofilm	1817:1823	arg1	structure					1825:1833	the overall biofilm structure	1805:1833	the overall biofilm structure	1805:1833	A better understanding of the contribution of individual matrix components to the overall biofilm structure will facilitate the development of more effective and specific methods to disrupt these bacterial communities.
24551241	0	37	theme	sectioned	56:64	arg1	biofilms					77:84	sectioned Salmonella biofilms	56:84	sectioned Salmonella biofilms on the surface of human gallstones	56:119	Visualization of extracellular matrix components within sectioned Salmonella biofilms on the surface of human gallstones.
24551241	10	38	theme	method	1624:1629	arg1	feasibility					1604:1614	feasibility	1604:1614	feasibility	1604:1614	These results demonstrate the use and feasibility of this method while highlighting the importance of studying the native architecture of the gallstone biofilm.
24551241	10	38	theme	method	1624:1629	arg1	use					1596:1598	use	1596:1598	use	1596:1598	These results demonstrate the use and feasibility of this method while highlighting the importance of studying the native architecture of the gallstone biofilm.
24551241	3	39	from	variable	484:491	arg1	response					496:503	response	496:503	response to altered environmental conditions	496:539	Previous work has demonstrated the composition of the biofilm matrix to be complex and highly variable in response to altered environmental conditions.
24551241	9	40	theme	preexisting	1488:1498	arg1	Enterobacteriaceae					1500:1517	preexisting Enterobacteriaceae	1488:1517	preexisting Enterobacteriaceae	1488:1517	Images also revealed the presence of preexisting Enterobacteriaceae encased within the structure of the gallstone.
24551241	6	41	theme	matrix	979:984	arg1	components					986:995	extracellular matrix components	965:995	extracellular matrix components of the Salmonella biofilm on the surface of human cholesterol gallstones	965:1068	In this work we describe a method for sectioning and direct visualization of extracellular matrix components of the Salmonella biofilm on the surface of human cholesterol gallstones and provide a description of the major matrix components observed therein.
24551241	11	42	theme	individual	1773:1782	arg1	components					1791:1800	individual matrix components	1773:1800	individual matrix components	1773:1800	A better understanding of the contribution of individual matrix components to the overall biofilm structure will facilitate the development of more effective and specific methods to disrupt these bacterial communities.
24551241	11	43	theme	better	1729:1734	arg1	understanding					1736:1748	A better understanding	1727:1748	A better understanding of the contribution of individual matrix components to the overall biofilm structure	1727:1833	A better understanding of the contribution of individual matrix components to the overall biofilm structure will facilitate the development of more effective and specific methods to disrupt these bacterial communities.
24551241	2	44	theme	macromolecular	367:380	arg1	matrix					382:387	a protective macromolecular matrix	354:387	a protective macromolecular matrix	354:387	Biofilms, by definition, involve the formation of a bacterial community encased within a protective macromolecular matrix.
24551241	9	45	theme	encased	1519:1525	arg1	presence					1476:1483	the presence	1472:1483	the presence of preexisting Enterobacteriaceae encased within the structure of the gallstone	1472:1563	Images also revealed the presence of preexisting Enterobacteriaceae encased within the structure of the gallstone.
24551241	11	46	theme	contribution	1757:1768	arg1	understanding					1736:1748	A better understanding	1727:1748	A better understanding of the contribution of individual matrix components to the overall biofilm structure	1727:1833	A better understanding of the contribution of individual matrix components to the overall biofilm structure will facilitate the development of more effective and specific methods to disrupt these bacterial communities.
24551241	7	47	theme	biofilm	1182:1188	arg1	formation					1190:1198	robust biofilm formation	1175:1198	robust biofilm formation	1175:1198	Confocal micrographs revealed robust biofilm formation, characterized by abundant but highly heterogeneous expression of polysaccharides such as LPS, Vi and O-antigen capsule.
24551241	6	48	theme	direct	941:946	arg1	visualization					948:960	direct visualization	941:960	direct visualization	941:960	In this work we describe a method for sectioning and direct visualization of extracellular matrix components of the Salmonella biofilm on the surface of human cholesterol gallstones and provide a description of the major matrix components observed therein.
24551241	10	49	theme	native	1681:1686	arg1	architecture					1688:1699	the native architecture	1677:1699	the native architecture of the gallstone biofilm	1677:1724	These results demonstrate the use and feasibility of this method while highlighting the importance of studying the native architecture of the gallstone biofilm.
24551241	7	50	theme	abundant	1218:1225	arg1	expression					1252:1261	abundant but highly heterogeneous expression	1218:1261	abundant but highly heterogeneous expression of polysaccharides such as LPS, Vi and O-antigen capsule	1218:1318	Confocal micrographs revealed robust biofilm formation, characterized by abundant but highly heterogeneous expression of polysaccharides such as LPS, Vi and O-antigen capsule.
24551241	11	51	theme	components	1791:1800	arg1	contribution					1757:1768	the contribution	1753:1768	the contribution of individual matrix components to the overall biofilm structure	1753:1833	A better understanding of the contribution of individual matrix components to the overall biofilm structure will facilitate the development of more effective and specific methods to disrupt these bacterial communities.
24551241	7	52	theme	Confocal	1145:1152	arg1	micrographs					1154:1164	Confocal micrographs	1145:1164	Confocal micrographs	1145:1164	Confocal micrographs revealed robust biofilm formation, characterized by abundant but highly heterogeneous expression of polysaccharides such as LPS, Vi and O-antigen capsule.
24551241	3	53	from	response	496:503	arg1	variable					484:491	variable	484:491	variable	484:491	Previous work has demonstrated the composition of the biofilm matrix to be complex and highly variable in response to altered environmental conditions.
24551241	2	54	theme	bacterial	319:327	arg1	community					329:337	a bacterial community	317:337	a bacterial community encased within a protective macromolecular matrix	317:387	Biofilms, by definition, involve the formation of a bacterial community encased within a protective macromolecular matrix.
24551241	9	55	theme	gallstone	1555:1563	arg1	structure					1538:1546	the structure	1534:1546	the structure of the gallstone	1534:1563	Images also revealed the presence of preexisting Enterobacteriaceae encased within the structure of the gallstone.
24551241	10	56	theme	gallstone	1708:1716	arg1	biofilm					1718:1724	the gallstone biofilm	1704:1724	the gallstone biofilm	1704:1724	These results demonstrate the use and feasibility of this method while highlighting the importance of studying the native architecture of the gallstone biofilm.
24551241	1	57	theme	bacterial	206:214	arg1	biofilms					216:223	bacterial biofilms	206:223	bacterial biofilms	206:223	Chronic carriage of Salmonella Typhi is mediated primarily through the formation of bacterial biofilms on the surface of cholesterol gallstones.
24551241	4	58	theme	Salmonella	638:647	arg1	matrix					657:662	the Salmonella biofilm matrix	634:662	the Salmonella biofilm matrix	634:662	Although known to play an important role in bacterial persistence in a variety of contexts, the Salmonella biofilm matrix remains largely uncharacterized under physiological conditions.
24551241	5	59	theme	matrix	754:759	arg1	components					761:770	matrix components	754:770	matrix components	754:770	Initial attempts to study matrix components and architecture of the biofilm matrix on gallstone surfaces were hindered by the auto-fluorescence of cholesterol.
24551241	6	60	theme	Salmonella	1004:1013	arg1	biofilm					1015:1021	the Salmonella biofilm	1000:1021	the Salmonella biofilm on the surface of human cholesterol gallstones	1000:1068	In this work we describe a method for sectioning and direct visualization of extracellular matrix components of the Salmonella biofilm on the surface of human cholesterol gallstones and provide a description of the major matrix components observed therein.
24551241	5	61	from	architecture	776:787	arg1	surfaces					824:831	gallstone surfaces	814:831	gallstone surfaces	814:831	Initial attempts to study matrix components and architecture of the biofilm matrix on gallstone surfaces were hindered by the auto-fluorescence of cholesterol.
24551241	1	62	theme	Chronic	122:128	arg1	carriage					130:137	Chronic carriage	122:137	Chronic carriage of Salmonella Typhi	122:157	Chronic carriage of Salmonella Typhi is mediated primarily through the formation of bacterial biofilms on the surface of cholesterol gallstones.
24551241	8	63	theme	flagellar	1369:1377	arg1	expression					1379:1388	flagellar expression	1369:1388	flagellar expression	1369:1388	CsgA was not observed in the biofilm matrix and flagellar expression was tightly restricted to the biofilm-cholesterol interface.
24551241	11	64	theme	specific	1889:1896	arg1	methods					1898:1904	more effective and specific methods	1870:1904	more effective and specific methods	1870:1904	A better understanding of the contribution of individual matrix components to the overall biofilm structure will facilitate the development of more effective and specific methods to disrupt these bacterial communities.
24551241	7	65	theme	O-antigen	1302:1310	arg1	LPS					1290:1292	LPS	1290:1292	LPS	1290:1292	Confocal micrographs revealed robust biofilm formation, characterized by abundant but highly heterogeneous expression of polysaccharides such as LPS, Vi and O-antigen capsule.
24551241	7	65	theme	O-antigen	1302:1310	arg1	capsule					1312:1318	O-antigen capsule	1302:1318	O-antigen capsule	1302:1318	Confocal micrographs revealed robust biofilm formation, characterized by abundant but highly heterogeneous expression of polysaccharides such as LPS, Vi and O-antigen capsule.
24551241	5	66	from	matrix	804:809	arg1	surfaces					824:831	gallstone surfaces	814:831	gallstone surfaces	814:831	Initial attempts to study matrix components and architecture of the biofilm matrix on gallstone surfaces were hindered by the auto-fluorescence of cholesterol.
24551241	1	67	theme	Typhi	153:157	arg1	carriage					130:137	Chronic carriage	122:137	Chronic carriage of Salmonella Typhi	122:157	Chronic carriage of Salmonella Typhi is mediated primarily through the formation of bacterial biofilms on the surface of cholesterol gallstones.
24551241	11	68	theme	effective	1875:1883	arg1	methods					1898:1904	more effective and specific methods	1870:1904	more effective and specific methods	1870:1904	A better understanding of the contribution of individual matrix components to the overall biofilm structure will facilitate the development of more effective and specific methods to disrupt these bacterial communities.
24551241	6	69	theme	gallstones	1059:1068	arg1	surface					1030:1036	the surface	1026:1036	the surface of human cholesterol gallstones	1026:1068	In this work we describe a method for sectioning and direct visualization of extracellular matrix components of the Salmonella biofilm on the surface of human cholesterol gallstones and provide a description of the major matrix components observed therein.
24551241	0	70	theme	human	104:108	arg1	gallstones					110:119	human gallstones	104:119	human gallstones	104:119	Visualization of extracellular matrix components within sectioned Salmonella biofilms on the surface of human gallstones.
24551241	6	71	theme	human	1041:1045	arg1	gallstones					1059:1068	human cholesterol gallstones	1041:1068	human cholesterol gallstones	1041:1068	In this work we describe a method for sectioning and direct visualization of extracellular matrix components of the Salmonella biofilm on the surface of human cholesterol gallstones and provide a description of the major matrix components observed therein.
24551241	5	72	dep	attempts	736:743	arg1	study					748:752	study	748:752	attempts to study matrix components	736:770	Initial attempts to study matrix components and architecture of the biofilm matrix on gallstone surfaces were hindered by the auto-fluorescence of cholesterol.
24551241	8	73	located	observed	1334:1341	arg1	matrix					1358:1363	the biofilm matrix	1346:1363	the biofilm matrix	1346:1363	CsgA was not observed in the biofilm matrix and flagellar expression was tightly restricted to the biofilm-cholesterol interface.
24551241	8	73	located	observed	1334:1341	arg2	CsgA					1321:1324	CsgA	1321:1324	CsgA	1321:1324	CsgA was not observed in the biofilm matrix and flagellar expression was tightly restricted to the biofilm-cholesterol interface.
24551241	3	74	theme	matrix	452:457	arg1	composition					425:435	the composition	421:435	the composition of the biofilm matrix	421:457	Previous work has demonstrated the composition of the biofilm matrix to be complex and highly variable in response to altered environmental conditions.
24551241	0	75	theme	matrix	31:36	arg1	components					38:47	extracellular matrix components	17:47	extracellular matrix components	17:47	Visualization of extracellular matrix components within sectioned Salmonella biofilms on the surface of human gallstones.
24551241	6	76	theme	matrix	1109:1114	arg1	components					1116:1125	the major matrix components	1099:1125	the major matrix components observed therein	1099:1142	In this work we describe a method for sectioning and direct visualization of extracellular matrix components of the Salmonella biofilm on the surface of human cholesterol gallstones and provide a description of the major matrix components observed therein.
24551241	4	77	theme	bacterial	586:594	arg1	persistence					596:606	bacterial persistence	586:606	bacterial persistence	586:606	Although known to play an important role in bacterial persistence in a variety of contexts, the Salmonella biofilm matrix remains largely uncharacterized under physiological conditions.
24551241	6	78	from	biofilm	1015:1021	arg1	surface					1030:1036	the surface	1026:1036	the surface of human cholesterol gallstones	1026:1068	In this work we describe a method for sectioning and direct visualization of extracellular matrix components of the Salmonella biofilm on the surface of human cholesterol gallstones and provide a description of the major matrix components observed therein.
24551241	11	79	theme	overall	1809:1815	arg1	structure					1825:1833	the overall biofilm structure	1805:1833	the overall biofilm structure	1805:1833	A better understanding of the contribution of individual matrix components to the overall biofilm structure will facilitate the development of more effective and specific methods to disrupt these bacterial communities.
24551241	1	80	theme	cholesterol	243:253	arg1	gallstones					255:264	cholesterol gallstones	243:264	cholesterol gallstones	243:264	Chronic carriage of Salmonella Typhi is mediated primarily through the formation of bacterial biofilms on the surface of cholesterol gallstones.
24551241	0	81	theme	Salmonella	66:75	arg1	biofilms					77:84	sectioned Salmonella biofilms	56:84	sectioned Salmonella biofilms on the surface of human gallstones	56:119	Visualization of extracellular matrix components within sectioned Salmonella biofilms on the surface of human gallstones.
24551241	6	82	from	components	986:995	arg1	surface					1030:1036	the surface	1026:1036	the surface of human cholesterol gallstones	1026:1068	In this work we describe a method for sectioning and direct visualization of extracellular matrix components of the Salmonella biofilm on the surface of human cholesterol gallstones and provide a description of the major matrix components observed therein.
24551241	3	83	theme	altered	508:514	arg1	conditions					530:539	altered environmental conditions	508:539	altered environmental conditions	508:539	Previous work has demonstrated the composition of the biofilm matrix to be complex and highly variable in response to altered environmental conditions.
24551241	4	84	theme	contexts	624:631	arg1	contexts					624:631	contexts	624:631	contexts	624:631	Although known to play an important role in bacterial persistence in a variety of contexts, the Salmonella biofilm matrix remains largely uncharacterized under physiological conditions.
24551241	4	84	theme	contexts	624:631	arg1	variety					613:619	a variety	611:619	a variety of contexts	611:631	Although known to play an important role in bacterial persistence in a variety of contexts, the Salmonella biofilm matrix remains largely uncharacterized under physiological conditions.
24551241	2	85	theme	community	329:337	arg1	formation					304:312	the formation	300:312	the formation of a bacterial community encased within a protective macromolecular matrix	300:387	Biofilms, by definition, involve the formation of a bacterial community encased within a protective macromolecular matrix.
24551241	1	86	theme	gallstones	255:264	arg1	surface					232:238	the surface	228:238	the surface of cholesterol gallstones	228:264	Chronic carriage of Salmonella Typhi is mediated primarily through the formation of bacterial biofilms on the surface of cholesterol gallstones.
24551241	9	87	theme	Enterobacteriaceae	1500:1517	arg1	presence					1476:1483	the presence	1472:1483	the presence of preexisting Enterobacteriaceae encased within the structure of the gallstone	1472:1563	Images also revealed the presence of preexisting Enterobacteriaceae encased within the structure of the gallstone.
26595665	5	0	theme	neutral	802:808	arg1	lipids					810:815	neutral lipids	802:815	neutral lipids	802:815	High amounts of glycogen were obtained in the mixotrophic cultures, while the amounts of neutral lipids were similar between mixotrophic and heterotrophic cultures.
26595665	1	1	theme	Galdieria	162:170	arg1	alga					157:160	The unicellular red alga	137:160	The unicellular red alga Galdieria sulphuraria	137:182	The unicellular red alga Galdieria sulphuraria grows efficiently and produces a large amount of biomass in acidic conditions at high temperatures.
26595665	5	2	theme	lipids	810:815	arg1	similar					822:828	similar	822:828	similar	822:828	High amounts of glycogen were obtained in the mixotrophic cultures, while the amounts of neutral lipids were similar between mixotrophic and heterotrophic cultures.
26595665	5	2	theme	lipids	810:815	arg1	amounts					791:797	the amounts	787:797	the amounts of neutral lipids	787:815	High amounts of glycogen were obtained in the mixotrophic cultures, while the amounts of neutral lipids were similar between mixotrophic and heterotrophic cultures.
26595665	5	2	theme	lipids	810:815	arg1	lipids					810:815	neutral lipids	802:815	neutral lipids	802:815	High amounts of glycogen were obtained in the mixotrophic cultures, while the amounts of neutral lipids were similar between mixotrophic and heterotrophic cultures.
26595665	1	3	theme	high	265:268	arg1	temperatures					270:281	high temperatures	265:281	high temperatures	265:281	The unicellular red alga Galdieria sulphuraria grows efficiently and produces a large amount of biomass in acidic conditions at high temperatures.
26595665	0	4	theme	red	105:107	arg1	sulphuraria					124:134	the sulfothermophilic red alga Galdieria sulphuraria	83:134	the sulfothermophilic red alga Galdieria sulphuraria	83:134	Profiling of lipid and glycogen accumulations under different growth conditions in the sulfothermophilic red alga Galdieria sulphuraria.
26595665	3	5	theme	lipids	545:550	arg1	compositions					516:527	compositions	516:527	compositions	516:527	In G. sulphuraria, biomass measurements and glycogen and lipid analyses demonstrated that the amounts and compositions of glycogen and lipids differed when cells were grown under autotrophic, mixotrophic, and heterotrophic conditions.
26595665	3	5	theme	lipids	545:550	arg1	amounts					504:510	amounts	504:510	amounts	504:510	In G. sulphuraria, biomass measurements and glycogen and lipid analyses demonstrated that the amounts and compositions of glycogen and lipids differed when cells were grown under autotrophic, mixotrophic, and heterotrophic conditions.
26595665	3	5	theme	lipids	545:550	arg1	lipids					545:550	lipids	545:550	lipids	545:550	In G. sulphuraria, biomass measurements and glycogen and lipid analyses demonstrated that the amounts and compositions of glycogen and lipids differed when cells were grown under autotrophic, mixotrophic, and heterotrophic conditions.
26595665	3	5	theme	lipids	545:550	arg1	glycogen					532:539	glycogen	532:539	glycogen	532:539	In G. sulphuraria, biomass measurements and glycogen and lipid analyses demonstrated that the amounts and compositions of glycogen and lipids differed when cells were grown under autotrophic, mixotrophic, and heterotrophic conditions.
26595665	3	6	dep	amounts	504:510	arg1	the					500:502	the	500:502	the	500:502	In G. sulphuraria, biomass measurements and glycogen and lipid analyses demonstrated that the amounts and compositions of glycogen and lipids differed when cells were grown under autotrophic, mixotrophic, and heterotrophic conditions.
26595665	0	7	theme	sulfothermophilic	87:103	arg1	sulphuraria					124:134	the sulfothermophilic red alga Galdieria sulphuraria	83:134	the sulfothermophilic red alga Galdieria sulphuraria	83:134	Profiling of lipid and glycogen accumulations under different growth conditions in the sulfothermophilic red alga Galdieria sulphuraria.
26595665	5	8	theme	High	713:716	arg1	glycogen					729:736	glycogen	729:736	glycogen	729:736	High amounts of glycogen were obtained in the mixotrophic cultures, while the amounts of neutral lipids were similar between mixotrophic and heterotrophic cultures.
26595665	5	8	theme	High	713:716	arg1	amounts					718:724	High amounts	713:724	High amounts of glycogen	713:736	High amounts of glycogen were obtained in the mixotrophic cultures, while the amounts of neutral lipids were similar between mixotrophic and heterotrophic cultures.
26595665	2	9	theme	great	291:295	arg1	potential					297:305	great potential	291:305	great potential	291:305	It has great potential to produce biofuels and other beneficial compounds without becoming contaminated with other organisms.
26595665	1	10	theme	unicellular	141:151	arg1	alga					157:160	The unicellular red alga	137:160	The unicellular red alga Galdieria sulphuraria	137:182	The unicellular red alga Galdieria sulphuraria grows efficiently and produces a large amount of biomass in acidic conditions at high temperatures.
26595665	0	11	theme	Galdieria	114:122	arg1	sulphuraria					124:134	the sulfothermophilic red alga Galdieria sulphuraria	83:134	the sulfothermophilic red alga Galdieria sulphuraria	83:134	Profiling of lipid and glycogen accumulations under different growth conditions in the sulfothermophilic red alga Galdieria sulphuraria.
26595665	7	12	dep	structure	968:976	arg1	compositions					994:1005	compositions	994:1005	compositions	994:1005	Glycogen structure and fatty acids compositions largely depended on the growth conditions.
26595665	0	13	theme	alga	109:112	arg1	sulphuraria					124:134	the sulfothermophilic red alga Galdieria sulphuraria	83:134	the sulfothermophilic red alga Galdieria sulphuraria	83:134	Profiling of lipid and glycogen accumulations under different growth conditions in the sulfothermophilic red alga Galdieria sulphuraria.
26595665	3	14	theme	biomass	429:435	arg1	measurements					437:448	biomass measurements	429:448	biomass measurements	429:448	In G. sulphuraria, biomass measurements and glycogen and lipid analyses demonstrated that the amounts and compositions of glycogen and lipids differed when cells were grown under autotrophic, mixotrophic, and heterotrophic conditions.
26595665	5	15	theme	heterotrophic	854:866	arg1	cultures					868:875	mixotrophic and heterotrophic cultures	838:875	mixotrophic and heterotrophic cultures	838:875	High amounts of glycogen were obtained in the mixotrophic cultures, while the amounts of neutral lipids were similar between mixotrophic and heterotrophic cultures.
26595665	3	16	theme	lipid	467:471	arg1	analyses					473:480	glycogen and lipid analyses	454:480	glycogen and lipid analyses	454:480	In G. sulphuraria, biomass measurements and glycogen and lipid analyses demonstrated that the amounts and compositions of glycogen and lipids differed when cells were grown under autotrophic, mixotrophic, and heterotrophic conditions.
26595665	6	17	theme	red	924:926	arg1	algae					928:932	red algae	924:932	red algae	924:932	The amounts of neutral lipids were highest in red algae, including thermophiles.
26595665	1	18	dep	Galdieria	162:170	arg1	sulphuraria					172:182	sulphuraria	172:182	sulphuraria	172:182	The unicellular red alga Galdieria sulphuraria grows efficiently and produces a large amount of biomass in acidic conditions at high temperatures.
26595665	3	19	theme	G.	413:414	arg1	sulphuraria					416:426	G. sulphuraria	413:426	G. sulphuraria	413:426	In G. sulphuraria, biomass measurements and glycogen and lipid analyses demonstrated that the amounts and compositions of glycogen and lipids differed when cells were grown under autotrophic, mixotrophic, and heterotrophic conditions.
26595665	3	20	theme	mixotrophic	602:612	arg1	conditions					633:642	autotrophic, mixotrophic, and heterotrophic conditions	589:642	autotrophic, mixotrophic, and heterotrophic conditions	589:642	In G. sulphuraria, biomass measurements and glycogen and lipid analyses demonstrated that the amounts and compositions of glycogen and lipids differed when cells were grown under autotrophic, mixotrophic, and heterotrophic conditions.
26595665	3	21	theme	glycogen	454:461	arg1	analyses					473:480	glycogen and lipid analyses	454:480	glycogen and lipid analyses	454:480	In G. sulphuraria, biomass measurements and glycogen and lipid analyses demonstrated that the amounts and compositions of glycogen and lipids differed when cells were grown under autotrophic, mixotrophic, and heterotrophic conditions.
26595665	2	22	contain	has	287:289	arg1	It					284:285	It	284:285	It	284:285	It has great potential to produce biofuels and other beneficial compounds without becoming contaminated with other organisms.
26595665	2	22	contain	has	287:289	arg2	potential					297:305	great potential	291:305	great potential	291:305	It has great potential to produce biofuels and other beneficial compounds without becoming contaminated with other organisms.
26595665	7	23	theme	fatty	982:986	arg1	acids					988:992	fatty acids	982:992	fatty acids	982:992	Glycogen structure and fatty acids compositions largely depended on the growth conditions.
26595665	0	24	theme	lipid	13:17	arg1	accumulations					32:44	lipid and glycogen accumulations	13:44	accumulations	32:44	Profiling of lipid and glycogen accumulations under different growth conditions in the sulfothermophilic red alga Galdieria sulphuraria.
26595665	3	25	theme	autotrophic	589:599	arg1	conditions					633:642	autotrophic, mixotrophic, and heterotrophic conditions	589:642	autotrophic, mixotrophic, and heterotrophic conditions	589:642	In G. sulphuraria, biomass measurements and glycogen and lipid analyses demonstrated that the amounts and compositions of glycogen and lipids differed when cells were grown under autotrophic, mixotrophic, and heterotrophic conditions.
26595665	1	26	theme	large	217:221	arg1	biomass					233:239	biomass	233:239	biomass	233:239	The unicellular red alga Galdieria sulphuraria grows efficiently and produces a large amount of biomass in acidic conditions at high temperatures.
26595665	1	26	theme	large	217:221	arg1	amount					223:228	a large amount	215:228	a large amount of biomass	215:239	The unicellular red alga Galdieria sulphuraria grows efficiently and produces a large amount of biomass in acidic conditions at high temperatures.
26595665	0	27	theme	accumulations	32:44	arg1	Profiling					0:8	Profiling	0:8	Profiling of lipid and glycogen accumulations under different growth conditions in the sulfothermophilic red alga Galdieria sulphuraria.	0:135	Profiling of lipid and glycogen accumulations under different growth conditions in the sulfothermophilic red alga Galdieria sulphuraria.
26595665	3	28	theme	heterotrophic	619:631	arg1	conditions					633:642	autotrophic, mixotrophic, and heterotrophic conditions	589:642	autotrophic, mixotrophic, and heterotrophic conditions	589:642	In G. sulphuraria, biomass measurements and glycogen and lipid analyses demonstrated that the amounts and compositions of glycogen and lipids differed when cells were grown under autotrophic, mixotrophic, and heterotrophic conditions.
26595665	0	29	theme	glycogen	23:30	arg1	accumulations					32:44	lipid and glycogen accumulations	13:44	accumulations	32:44	Profiling of lipid and glycogen accumulations under different growth conditions in the sulfothermophilic red alga Galdieria sulphuraria.
26595665	5	30	theme	mixotrophic	759:769	arg1	cultures					771:778	the mixotrophic cultures	755:778	the mixotrophic cultures	755:778	High amounts of glycogen were obtained in the mixotrophic cultures, while the amounts of neutral lipids were similar between mixotrophic and heterotrophic cultures.
26595665	0	31	from	conditions	69:78	arg1	sulphuraria					124:134	the sulfothermophilic red alga Galdieria sulphuraria	83:134	the sulfothermophilic red alga Galdieria sulphuraria	83:134	Profiling of lipid and glycogen accumulations under different growth conditions in the sulfothermophilic red alga Galdieria sulphuraria.
26595665	0	32	theme	different	52:60	arg1	conditions					69:78	different growth conditions	52:78	different growth conditions in the sulfothermophilic red alga Galdieria sulphuraria	52:134	Profiling of lipid and glycogen accumulations under different growth conditions in the sulfothermophilic red alga Galdieria sulphuraria.
26595665	3	33	theme	glycogen	532:539	arg1	compositions					516:527	compositions	516:527	compositions	516:527	In G. sulphuraria, biomass measurements and glycogen and lipid analyses demonstrated that the amounts and compositions of glycogen and lipids differed when cells were grown under autotrophic, mixotrophic, and heterotrophic conditions.
26595665	3	33	theme	glycogen	532:539	arg1	amounts					504:510	amounts	504:510	amounts	504:510	In G. sulphuraria, biomass measurements and glycogen and lipid analyses demonstrated that the amounts and compositions of glycogen and lipids differed when cells were grown under autotrophic, mixotrophic, and heterotrophic conditions.
26595665	3	33	theme	glycogen	532:539	arg1	lipids					545:550	lipids	545:550	lipids	545:550	In G. sulphuraria, biomass measurements and glycogen and lipid analyses demonstrated that the amounts and compositions of glycogen and lipids differed when cells were grown under autotrophic, mixotrophic, and heterotrophic conditions.
26595665	3	33	theme	glycogen	532:539	arg1	glycogen					532:539	glycogen	532:539	glycogen	532:539	In G. sulphuraria, biomass measurements and glycogen and lipid analyses demonstrated that the amounts and compositions of glycogen and lipids differed when cells were grown under autotrophic, mixotrophic, and heterotrophic conditions.
26595665	6	34	theme	lipids	901:906	arg1	highest					913:919	highest	913:919	highest	913:919	The amounts of neutral lipids were highest in red algae, including thermophiles.
26595665	6	34	theme	lipids	901:906	arg1	amounts					882:888	The amounts	878:888	The amounts of neutral lipids	878:906	The amounts of neutral lipids were highest in red algae, including thermophiles.
26595665	6	34	theme	lipids	901:906	arg1	lipids					901:906	neutral lipids	893:906	neutral lipids	893:906	The amounts of neutral lipids were highest in red algae, including thermophiles.
26595665	5	35	theme	mixotrophic	838:848	arg1	cultures					868:875	mixotrophic and heterotrophic cultures	838:875	mixotrophic and heterotrophic cultures	838:875	High amounts of glycogen were obtained in the mixotrophic cultures, while the amounts of neutral lipids were similar between mixotrophic and heterotrophic cultures.
26595665	6	36	theme	neutral	893:899	arg1	lipids					901:906	neutral lipids	893:906	neutral lipids	893:906	The amounts of neutral lipids were highest in red algae, including thermophiles.
26595665	7	37	theme	Glycogen	959:966	arg1	structure					968:976	Glycogen structure	959:976	Glycogen structure	959:976	Glycogen structure and fatty acids compositions largely depended on the growth conditions.
26595665	5	38	theme	glycogen	729:736	arg1	glycogen					729:736	glycogen	729:736	glycogen	729:736	High amounts of glycogen were obtained in the mixotrophic cultures, while the amounts of neutral lipids were similar between mixotrophic and heterotrophic cultures.
26595665	5	38	theme	glycogen	729:736	arg1	amounts					718:724	High amounts	713:724	High amounts of glycogen	713:736	High amounts of glycogen were obtained in the mixotrophic cultures, while the amounts of neutral lipids were similar between mixotrophic and heterotrophic cultures.
26595665	1	39	theme	red	153:155	arg1	alga					157:160	The unicellular red alga	137:160	The unicellular red alga Galdieria sulphuraria	137:182	The unicellular red alga Galdieria sulphuraria grows efficiently and produces a large amount of biomass in acidic conditions at high temperatures.
26595665	2	40	theme	beneficial	337:346	arg1	compounds					348:356	other beneficial compounds	331:356	other beneficial compounds	331:356	It has great potential to produce biofuels and other beneficial compounds without becoming contaminated with other organisms.
26595665	1	41	theme	biomass	233:239	arg1	biomass					233:239	biomass	233:239	biomass	233:239	The unicellular red alga Galdieria sulphuraria grows efficiently and produces a large amount of biomass in acidic conditions at high temperatures.
26595665	1	41	theme	biomass	233:239	arg1	amount					223:228	a large amount	215:228	a large amount of biomass	215:239	The unicellular red alga Galdieria sulphuraria grows efficiently and produces a large amount of biomass in acidic conditions at high temperatures.
26595665	2	42	theme	other	331:335	arg1	compounds					348:356	other beneficial compounds	331:356	other beneficial compounds	331:356	It has great potential to produce biofuels and other beneficial compounds without becoming contaminated with other organisms.
26595665	2	43	theme	other	393:397	arg1	organisms					399:407	other organisms	393:407	other organisms	393:407	It has great potential to produce biofuels and other beneficial compounds without becoming contaminated with other organisms.
26595665	4	44	theme	mixotrophic	692:702	arg1	culture					704:710	the mixotrophic culture	688:710	the mixotrophic culture	688:710	Maximum biomass production was obtained in the mixotrophic culture.
26595665	1	45	theme	acidic	244:249	arg1	conditions					251:260	acidic conditions	244:260	acidic conditions	244:260	The unicellular red alga Galdieria sulphuraria grows efficiently and produces a large amount of biomass in acidic conditions at high temperatures.
26595665	0	46	theme	growth	62:67	arg1	conditions					69:78	different growth conditions	52:78	different growth conditions in the sulfothermophilic red alga Galdieria sulphuraria	52:134	Profiling of lipid and glycogen accumulations under different growth conditions in the sulfothermophilic red alga Galdieria sulphuraria.
26595665	7	47	theme	growth	1031:1036	arg1	conditions					1038:1047	the growth conditions	1027:1047	the growth conditions	1027:1047	Glycogen structure and fatty acids compositions largely depended on the growth conditions.
26595665	4	48	theme	Maximum	645:651	arg1	production					661:670	Maximum biomass production	645:670	Maximum biomass production	645:670	Maximum biomass production was obtained in the mixotrophic culture.
26595665	4	49	theme	biomass	653:659	arg1	production					661:670	Maximum biomass production	645:670	Maximum biomass production	645:670	Maximum biomass production was obtained in the mixotrophic culture.
27315087	10	0	theme	BA	2063:2064	arg1	formation					2066:2074	cecal BA formation	2057:2074	cecal BA formation	2057:2074	These results indicate that the molecular properties of guar gum and pectin are important for their ability to modulate cecal BA formation, gut microbiota composition, and high-fat diet induced inflammation.
27315087	4	1	theme	MiSeq	934:938	arg1	platform					940:947	the Illumina MiSeq platform	921:947	the Illumina MiSeq platform	921:947	Cecal BAs, short-chain fatty acids (SCFA) and microbiota composition, and plasma lipopolysaccharide-binding protein (LBP) levels were analyzed, by using novel methodologies based on gas chromatography (BAs and SCFAs) and 16S rRNA gene sequencing on the Illumina MiSeq platform.
27315087	9	2	theme	unclassified	1908:1919	arg1	family					1921:1926	an unclassified family	1905:1926	an unclassified family in RF32	1905:1934	The inflammatory marker LBP was downregulated in rats fed medium-MW guar gum and HM pectin; these two fibers decreased the cecal abundance of Oscillospira and an unclassified genus in Ruminococcaceae, and increased that of an unclassified family in RF32.
27315087	6	3	theme	cecal	1158:1162	arg1	acids					1223:1227	cholic-, chenodeoxycholic-, and ursodeoxycholic acids	1175:1227	cholic-, chenodeoxycholic-, and ursodeoxycholic acids as well as α-, β-, and ω-muricholic acids	1175:1269	Notably, guar gum consumption with medium-MW increased the cecal amounts of cholic-, chenodeoxycholic-, and ursodeoxycholic acids as well as α-, β-, and ω-muricholic acids to a greater extent than other types of guar gum or the fiber-free control diet.
27315087	6	3	theme	cecal	1158:1162	arg1	acids					1265:1269	α-, β-, and ω-muricholic acids	1240:1269	cholic-, chenodeoxycholic-, and ursodeoxycholic acids as well as α-, β-, and ω-muricholic acids	1175:1269	Notably, guar gum consumption with medium-MW increased the cecal amounts of cholic-, chenodeoxycholic-, and ursodeoxycholic acids as well as α-, β-, and ω-muricholic acids to a greater extent than other types of guar gum or the fiber-free control diet.
27315087	6	3	theme	cecal	1158:1162	arg1	amounts					1164:1170	the cecal amounts	1154:1170	the cecal amounts of cholic-, chenodeoxycholic-, and ursodeoxycholic acids as well as α-, β-, and ω-muricholic acids	1154:1269	Notably, guar gum consumption with medium-MW increased the cecal amounts of cholic-, chenodeoxycholic-, and ursodeoxycholic acids as well as α-, β-, and ω-muricholic acids to a greater extent than other types of guar gum or the fiber-free control diet.
27315087	8	4	theme	pectin	1530:1535	arg1	groups					1537:1542	pectin groups	1530:1542	pectin groups	1530:1542	Differences in BA composition between pectin groups were less obvious, but cecal levels of α- and ω-muricholic acids were higher in rats fed LM as compared to HM pectin or the control diet.
27315087	9	5	theme	marker	1699:1704	arg1	LBP					1706:1708	The inflammatory marker LBP	1682:1708	The inflammatory marker LBP	1682:1708	The inflammatory marker LBP was downregulated in rats fed medium-MW guar gum and HM pectin; these two fibers decreased the cecal abundance of Oscillospira and an unclassified genus in Ruminococcaceae, and increased that of an unclassified family in RF32.
27315087	3	6	theme	low-	648:651	arg1	diets					665:669	either low- or high-fat diets	641:669	either low- or high-fat diets	641:669	Low- (LM) or high-methoxylated (HM) pectin, and low-, medium-, or high-molecular-weight (MW) guar gum were administered to rats that were fed either low- or high-fat diets.
27315087	8	7	theme	control	1668:1674	arg1	diet					1676:1679	the control diet	1664:1679	the control diet	1664:1679	Differences in BA composition between pectin groups were less obvious, but cecal levels of α- and ω-muricholic acids were higher in rats fed LM as compared to HM pectin or the control diet.
27315087	1	8	theme	colonic	235:241	arg1	composition					254:264	colonic microbiota composition	235:264	colonic microbiota composition	235:264	Bile acids (BAs) act as signaling molecules in various physiological processes, and are related to colonic microbiota composition as well as to different types of dietary fat and fiber.
27315087	4	9	theme	plasma	746:751	arg1	LBP					789:791	LBP	789:791	LBP	789:791	Cecal BAs, short-chain fatty acids (SCFA) and microbiota composition, and plasma lipopolysaccharide-binding protein (LBP) levels were analyzed, by using novel methodologies based on gas chromatography (BAs and SCFAs) and 16S rRNA gene sequencing on the Illumina MiSeq platform.
27315087	4	9	theme	plasma	746:751	arg1	protein					780:786	plasma lipopolysaccharide-binding protein	746:786	plasma lipopolysaccharide-binding protein (LBP) levels	746:799	Cecal BAs, short-chain fatty acids (SCFA) and microbiota composition, and plasma lipopolysaccharide-binding protein (LBP) levels were analyzed, by using novel methodologies based on gas chromatography (BAs and SCFAs) and 16S rRNA gene sequencing on the Illumina MiSeq platform.
27315087	8	10	theme	BA	1507:1508	arg1	composition					1510:1520	BA composition	1507:1520	BA composition	1507:1520	Differences in BA composition between pectin groups were less obvious, but cecal levels of α- and ω-muricholic acids were higher in rats fed LM as compared to HM pectin or the control diet.
27315087	5	11	from	rats	1074:1077	arg1	diet					1093:1096	a high-fat diet	1082:1096	a high-fat diet	1082:1096	Strong correlations were observed between cecal BA and SCFA levels, microbiota composition, and portal plasma LBP levels in rats on a high-fat diet.
27315087	2	12	with	fibers	378:383	arg1	metabolites					478:488	gut metabolites	474:488	gut metabolites	474:488	This study investigated whether guar gum and pectin-two fibers with distinct functional characteristics-affect BA profiles, microbiota composition, and gut metabolites in rats.
27315087	2	12	with	fibers	378:383	arg1	profiles					436:443	distinct functional characteristics-affect BA profiles	390:443	distinct functional characteristics-affect BA profiles	390:443	This study investigated whether guar gum and pectin-two fibers with distinct functional characteristics-affect BA profiles, microbiota composition, and gut metabolites in rats.
27315087	2	12	with	fibers	378:383	arg1	composition					457:467	microbiota composition	446:467	microbiota composition	446:467	This study investigated whether guar gum and pectin-two fibers with distinct functional characteristics-affect BA profiles, microbiota composition, and gut metabolites in rats.
27315087	4	13	theme	protein	780:786	arg1	levels					794:799	plasma lipopolysaccharide-binding protein (LBP) levels	746:799	plasma lipopolysaccharide-binding protein (LBP) levels	746:799	Cecal BAs, short-chain fatty acids (SCFA) and microbiota composition, and plasma lipopolysaccharide-binding protein (LBP) levels were analyzed, by using novel methodologies based on gas chromatography (BAs and SCFAs) and 16S rRNA gene sequencing on the Illumina MiSeq platform.
27315087	1	14	theme	microbiota	243:252	arg1	composition					254:264	colonic microbiota composition	235:264	colonic microbiota composition	235:264	Bile acids (BAs) act as signaling molecules in various physiological processes, and are related to colonic microbiota composition as well as to different types of dietary fat and fiber.
27315087	5	15	from	levels	1064:1069	arg1	diet					1093:1096	a high-fat diet	1082:1096	a high-fat diet	1082:1096	Strong correlations were observed between cecal BA and SCFA levels, microbiota composition, and portal plasma LBP levels in rats on a high-fat diet.
27315087	5	15	from	levels	1064:1069	arg1	rats					1074:1077	rats	1074:1077	rats on a high-fat diet	1074:1096	Strong correlations were observed between cecal BA and SCFA levels, microbiota composition, and portal plasma LBP levels in rats on a high-fat diet.
27315087	7	16	theme	chain	1478:1482	arg1	length					1484:1489	chain length	1478:1489	chain length	1478:1489	In contrast, the amounts of cecal deoxycholic- and hyodeoxycholic acid were reduced with all types of guar gum independent of chain length.
27315087	4	17	theme	novel	825:829	arg1	methodologies					831:843	novel methodologies	825:843	novel methodologies based on gas chromatography (BAs and SCFAs) and 16S rRNA gene sequencing on the Illumina MiSeq platform	825:947	Cecal BAs, short-chain fatty acids (SCFA) and microbiota composition, and plasma lipopolysaccharide-binding protein (LBP) levels were analyzed, by using novel methodologies based on gas chromatography (BAs and SCFAs) and 16S rRNA gene sequencing on the Illumina MiSeq platform.
27315087	3	18	theme	high-fat	656:663	arg1	diets					665:669	either low- or high-fat diets	641:669	either low- or high-fat diets	641:669	Low- (LM) or high-methoxylated (HM) pectin, and low-, medium-, or high-molecular-weight (MW) guar gum were administered to rats that were fed either low- or high-fat diets.
27315087	4	19	dep	chromatography	858:871	arg1	SCFAs					882:886	SCFAs	882:886	SCFAs	882:886	Cecal BAs, short-chain fatty acids (SCFA) and microbiota composition, and plasma lipopolysaccharide-binding protein (LBP) levels were analyzed, by using novel methodologies based on gas chromatography (BAs and SCFAs) and 16S rRNA gene sequencing on the Illumina MiSeq platform.
27315087	4	19	dep	chromatography	858:871	arg1	BAs					874:876	BAs	874:876	BAs	874:876	Cecal BAs, short-chain fatty acids (SCFA) and microbiota composition, and plasma lipopolysaccharide-binding protein (LBP) levels were analyzed, by using novel methodologies based on gas chromatography (BAs and SCFAs) and 16S rRNA gene sequencing on the Illumina MiSeq platform.
27315087	7	20	theme	independent	1463:1473	arg1	types					1445:1449	all types	1441:1449	all types of guar gum independent of chain length	1441:1489	In contrast, the amounts of cecal deoxycholic- and hyodeoxycholic acid were reduced with all types of guar gum independent of chain length.
27315087	0	21	from	Acids	62:66	arg1	Rats					130:133	Rats	130:133	Rats	130:133	Molecular Properties of Guar Gum and Pectin Modify Cecal Bile Acids, Microbiota, and Plasma Lipopolysaccharide-Binding Protein in Rats.
27315087	6	22	theme	guar	1108:1111	arg1	consumption					1117:1127	guar gum consumption	1108:1127	guar gum consumption with medium-MW	1108:1142	Notably, guar gum consumption with medium-MW increased the cecal amounts of cholic-, chenodeoxycholic-, and ursodeoxycholic acids as well as α-, β-, and ω-muricholic acids to a greater extent than other types of guar gum or the fiber-free control diet.
27315087	9	23	theme	guar	1750:1753	arg1	gum					1755:1757	medium-MW guar gum	1740:1757	medium-MW guar gum	1740:1757	The inflammatory marker LBP was downregulated in rats fed medium-MW guar gum and HM pectin; these two fibers decreased the cecal abundance of Oscillospira and an unclassified genus in Ruminococcaceae, and increased that of an unclassified family in RF32.
27315087	2	24	theme	functional	399:408	arg1	profiles					436:443	distinct functional characteristics-affect BA profiles	390:443	distinct functional characteristics-affect BA profiles	390:443	This study investigated whether guar gum and pectin-two fibers with distinct functional characteristics-affect BA profiles, microbiota composition, and gut metabolites in rats.
27315087	8	25	theme	α-	1583:1584	arg1	acids					1603:1607	α- and ω-muricholic acids	1583:1607	α- and ω-muricholic acids	1583:1607	Differences in BA composition between pectin groups were less obvious, but cecal levels of α- and ω-muricholic acids were higher in rats fed LM as compared to HM pectin or the control diet.
27315087	1	26	theme	various	183:189	arg1	processes					205:213	various physiological processes	183:213	various physiological processes	183:213	Bile acids (BAs) act as signaling molecules in various physiological processes, and are related to colonic microbiota composition as well as to different types of dietary fat and fiber.
27315087	7	27	theme	guar	1454:1457	arg1	gum					1459:1461	guar gum	1454:1461	guar gum	1454:1461	In contrast, the amounts of cecal deoxycholic- and hyodeoxycholic acid were reduced with all types of guar gum independent of chain length.
27315087	8	28	theme	ω-muricholic	1590:1601	arg1	acids					1603:1607	α- and ω-muricholic acids	1583:1607	α- and ω-muricholic acids	1583:1607	Differences in BA composition between pectin groups were less obvious, but cecal levels of α- and ω-muricholic acids were higher in rats fed LM as compared to HM pectin or the control diet.
27315087	0	29	from	Microbiota	69:78	arg1	Rats					130:133	Rats	130:133	Rats	130:133	Molecular Properties of Guar Gum and Pectin Modify Cecal Bile Acids, Microbiota, and Plasma Lipopolysaccharide-Binding Protein in Rats.
27315087	0	30	theme	Cecal	51:55	arg1	Acids					62:66	Cecal Bile Acids	51:66	Cecal Bile Acids	51:66	Molecular Properties of Guar Gum and Pectin Modify Cecal Bile Acids, Microbiota, and Plasma Lipopolysaccharide-Binding Protein in Rats.
27315087	3	31	theme	medium-	553:559	arg1	MW					588:589	MW	588:589	MW	588:589	Low- (LM) or high-methoxylated (HM) pectin, and low-, medium-, or high-molecular-weight (MW) guar gum were administered to rats that were fed either low- or high-fat diets.
27315087	3	31	theme	medium-	553:559	arg1	gum					597:599	low-, medium-, or high-molecular-weight (MW) guar gum	547:599	low-, medium-, or high-molecular-weight (MW) guar gum	547:599	Low- (LM) or high-methoxylated (HM) pectin, and low-, medium-, or high-molecular-weight (MW) guar gum were administered to rats that were fed either low- or high-fat diets.
27315087	1	32	theme	Bile	136:139	arg1	molecules					170:178	signaling molecules	160:178	signaling molecules in various physiological processes	160:213	Bile acids (BAs) act as signaling molecules in various physiological processes, and are related to colonic microbiota composition as well as to different types of dietary fat and fiber.
27315087	1	32	theme	Bile	136:139	arg1	BAs					148:150	BAs	148:150	BAs	148:150	Bile acids (BAs) act as signaling molecules in various physiological processes, and are related to colonic microbiota composition as well as to different types of dietary fat and fiber.
27315087	1	32	theme	Bile	136:139	arg1	acids					141:145	Bile acids	136:145	Bile acids (BAs)	136:151	Bile acids (BAs) act as signaling molecules in various physiological processes, and are related to colonic microbiota composition as well as to different types of dietary fat and fiber.
27315087	10	33	theme	molecular	1969:1977	arg1	properties					1979:1988	the molecular properties	1965:1988	the molecular properties of guar gum and pectin	1965:2011	These results indicate that the molecular properties of guar gum and pectin are important for their ability to modulate cecal BA formation, gut microbiota composition, and high-fat diet induced inflammation.
27315087	10	33	theme	molecular	1969:1977	arg1	important					2017:2025	important	2017:2025	important	2017:2025	These results indicate that the molecular properties of guar gum and pectin are important for their ability to modulate cecal BA formation, gut microbiota composition, and high-fat diet induced inflammation.
27315087	1	34	theme	fat	307:309	arg1	types					290:294	different types	280:294	different types of dietary fat and fiber	280:319	Bile acids (BAs) act as signaling molecules in various physiological processes, and are related to colonic microbiota composition as well as to different types of dietary fat and fiber.
27315087	7	35	theme	hyodeoxycholic	1403:1416	arg1	acid					1418:1421	hyodeoxycholic acid	1403:1421	hyodeoxycholic acid	1403:1421	In contrast, the amounts of cecal deoxycholic- and hyodeoxycholic acid were reduced with all types of guar gum independent of chain length.
27315087	4	36	theme	gas	854:856	arg1	chromatography					858:871	gas chromatography	854:871	gas chromatography (BAs and SCFAs)	854:887	Cecal BAs, short-chain fatty acids (SCFA) and microbiota composition, and plasma lipopolysaccharide-binding protein (LBP) levels were analyzed, by using novel methodologies based on gas chromatography (BAs and SCFAs) and 16S rRNA gene sequencing on the Illumina MiSeq platform.
27315087	10	37	theme	pectin	2006:2011	arg1	properties					1979:1988	the molecular properties	1965:1988	the molecular properties of guar gum and pectin	1965:2011	These results indicate that the molecular properties of guar gum and pectin are important for their ability to modulate cecal BA formation, gut microbiota composition, and high-fat diet induced inflammation.
27315087	10	37	theme	pectin	2006:2011	arg1	important					2017:2025	important	2017:2025	important	2017:2025	These results indicate that the molecular properties of guar gum and pectin are important for their ability to modulate cecal BA formation, gut microbiota composition, and high-fat diet induced inflammation.
27315087	1	38	theme	fiber	315:319	arg1	types					290:294	different types	280:294	different types of dietary fat and fiber	280:319	Bile acids (BAs) act as signaling molecules in various physiological processes, and are related to colonic microbiota composition as well as to different types of dietary fat and fiber.
27315087	7	39	theme	deoxycholic-	1386:1397	arg1	amounts					1369:1375	the amounts	1365:1375	the amounts of cecal deoxycholic- and hyodeoxycholic acid	1365:1421	In contrast, the amounts of cecal deoxycholic- and hyodeoxycholic acid were reduced with all types of guar gum independent of chain length.
27315087	7	39	theme	deoxycholic-	1386:1397	arg1	deoxycholic-					1386:1397	cecal deoxycholic-	1380:1397	cecal deoxycholic-	1380:1397	In contrast, the amounts of cecal deoxycholic- and hyodeoxycholic acid were reduced with all types of guar gum independent of chain length.
27315087	7	39	theme	deoxycholic-	1386:1397	arg1	acid					1418:1421	hyodeoxycholic acid	1403:1421	hyodeoxycholic acid	1403:1421	In contrast, the amounts of cecal deoxycholic- and hyodeoxycholic acid were reduced with all types of guar gum independent of chain length.
27315087	2	40	from	profiles	436:443	arg1	rats					493:496	rats	493:496	rats	493:496	This study investigated whether guar gum and pectin-two fibers with distinct functional characteristics-affect BA profiles, microbiota composition, and gut metabolites in rats.
27315087	10	41	theme	gum	1998:2000	arg1	properties					1979:1988	the molecular properties	1965:1988	the molecular properties of guar gum and pectin	1965:2011	These results indicate that the molecular properties of guar gum and pectin are important for their ability to modulate cecal BA formation, gut microbiota composition, and high-fat diet induced inflammation.
27315087	10	41	theme	gum	1998:2000	arg1	important					2017:2025	important	2017:2025	important	2017:2025	These results indicate that the molecular properties of guar gum and pectin are important for their ability to modulate cecal BA formation, gut microbiota composition, and high-fat diet induced inflammation.
27315087	4	42	theme	fatty	695:699	arg1	SCFA					708:711	SCFA	708:711	SCFA	708:711	Cecal BAs, short-chain fatty acids (SCFA) and microbiota composition, and plasma lipopolysaccharide-binding protein (LBP) levels were analyzed, by using novel methodologies based on gas chromatography (BAs and SCFAs) and 16S rRNA gene sequencing on the Illumina MiSeq platform.
27315087	4	42	theme	fatty	695:699	arg1	acids					701:705	short-chain fatty acids	683:705	short-chain fatty acids (SCFA)	683:712	Cecal BAs, short-chain fatty acids (SCFA) and microbiota composition, and plasma lipopolysaccharide-binding protein (LBP) levels were analyzed, by using novel methodologies based on gas chromatography (BAs and SCFAs) and 16S rRNA gene sequencing on the Illumina MiSeq platform.
27315087	2	43	theme	gum	359:361	arg1	fibers					378:383	guar gum and pectin-two fibers	354:383	guar gum and pectin-two fibers with distinct functional characteristics-affect BA profiles, microbiota composition, and gut metabolites in rats	354:496	This study investigated whether guar gum and pectin-two fibers with distinct functional characteristics-affect BA profiles, microbiota composition, and gut metabolites in rats.
27315087	9	44	from	genus	1857:1861	arg1	Ruminococcaceae					1866:1880	Ruminococcaceae	1866:1880	Ruminococcaceae	1866:1880	The inflammatory marker LBP was downregulated in rats fed medium-MW guar gum and HM pectin; these two fibers decreased the cecal abundance of Oscillospira and an unclassified genus in Ruminococcaceae, and increased that of an unclassified family in RF32.
27315087	2	45	theme	BA	433:434	arg1	profiles					436:443	distinct functional characteristics-affect BA profiles	390:443	distinct functional characteristics-affect BA profiles	390:443	This study investigated whether guar gum and pectin-two fibers with distinct functional characteristics-affect BA profiles, microbiota composition, and gut metabolites in rats.
27315087	9	46	theme	Oscillospira	1824:1835	arg1	abundance					1811:1819	the cecal abundance	1801:1819	the cecal abundance of Oscillospira	1801:1835	The inflammatory marker LBP was downregulated in rats fed medium-MW guar gum and HM pectin; these two fibers decreased the cecal abundance of Oscillospira and an unclassified genus in Ruminococcaceae, and increased that of an unclassified family in RF32.
27315087	9	46	theme	Oscillospira	1824:1835	arg1	genus					1857:1861	an unclassified genus	1841:1861	an unclassified genus in Ruminococcaceae	1841:1880	The inflammatory marker LBP was downregulated in rats fed medium-MW guar gum and HM pectin; these two fibers decreased the cecal abundance of Oscillospira and an unclassified genus in Ruminococcaceae, and increased that of an unclassified family in RF32.
27315087	0	47	theme	Molecular	0:8	arg1	Properties					10:19	Molecular Properties	0:19	Molecular Properties of Guar Gum and Pectin	0:42	Molecular Properties of Guar Gum and Pectin Modify Cecal Bile Acids, Microbiota, and Plasma Lipopolysaccharide-Binding Protein in Rats.
27315087	5	48	theme	LBP	1060:1062	arg1	levels					1064:1069	portal plasma LBP levels	1046:1069	portal plasma LBP levels	1046:1069	Strong correlations were observed between cecal BA and SCFA levels, microbiota composition, and portal plasma LBP levels in rats on a high-fat diet.
27315087	10	49	theme	microbiota	2081:2090	arg1	composition					2092:2102	gut microbiota composition	2077:2102	gut microbiota composition	2077:2102	These results indicate that the molecular properties of guar gum and pectin are important for their ability to modulate cecal BA formation, gut microbiota composition, and high-fat diet induced inflammation.
27315087	6	50	theme	ursodeoxycholic	1207:1221	arg1	acids					1223:1227	cholic-, chenodeoxycholic-, and ursodeoxycholic acids	1175:1227	cholic-, chenodeoxycholic-, and ursodeoxycholic acids as well as α-, β-, and ω-muricholic acids	1175:1269	Notably, guar gum consumption with medium-MW increased the cecal amounts of cholic-, chenodeoxycholic-, and ursodeoxycholic acids as well as α-, β-, and ω-muricholic acids to a greater extent than other types of guar gum or the fiber-free control diet.
27315087	4	51	theme	Illumina	925:932	arg1	platform					940:947	the Illumina MiSeq platform	921:947	the Illumina MiSeq platform	921:947	Cecal BAs, short-chain fatty acids (SCFA) and microbiota composition, and plasma lipopolysaccharide-binding protein (LBP) levels were analyzed, by using novel methodologies based on gas chromatography (BAs and SCFAs) and 16S rRNA gene sequencing on the Illumina MiSeq platform.
27315087	4	52	theme	Cecal	672:676	arg1	BAs					678:680	Cecal BAs	672:680	Cecal BAs	672:680	Cecal BAs, short-chain fatty acids (SCFA) and microbiota composition, and plasma lipopolysaccharide-binding protein (LBP) levels were analyzed, by using novel methodologies based on gas chromatography (BAs and SCFAs) and 16S rRNA gene sequencing on the Illumina MiSeq platform.
27315087	6	53	theme	greater	1276:1282	arg1	extent					1284:1289	a greater extent	1274:1289	a greater extent than other types of guar gum or the fiber-free control diet	1274:1349	Notably, guar gum consumption with medium-MW increased the cecal amounts of cholic-, chenodeoxycholic-, and ursodeoxycholic acids as well as α-, β-, and ω-muricholic acids to a greater extent than other types of guar gum or the fiber-free control diet.
27315087	3	54	dep	Low-	499:502	arg1	LM					505:506	LM	505:506	LM	505:506	Low- (LM) or high-methoxylated (HM) pectin, and low-, medium-, or high-molecular-weight (MW) guar gum were administered to rats that were fed either low- or high-fat diets.
27315087	7	55	theme	length	1484:1489	arg1	independent					1463:1473	independent	1463:1473	independent	1463:1473	In contrast, the amounts of cecal deoxycholic- and hyodeoxycholic acid were reduced with all types of guar gum independent of chain length.
27315087	4	56	theme	16S	893:895	arg1	sequencing					907:916	16S rRNA gene sequencing	893:916	16S rRNA gene sequencing	893:916	Cecal BAs, short-chain fatty acids (SCFA) and microbiota composition, and plasma lipopolysaccharide-binding protein (LBP) levels were analyzed, by using novel methodologies based on gas chromatography (BAs and SCFAs) and 16S rRNA gene sequencing on the Illumina MiSeq platform.
27315087	4	57	theme	microbiota	718:727	arg1	composition					729:739	microbiota composition	718:739	microbiota composition	718:739	Cecal BAs, short-chain fatty acids (SCFA) and microbiota composition, and plasma lipopolysaccharide-binding protein (LBP) levels were analyzed, by using novel methodologies based on gas chromatography (BAs and SCFAs) and 16S rRNA gene sequencing on the Illumina MiSeq platform.
27315087	2	58	theme	pectin-two	367:376	arg1	fibers					378:383	guar gum and pectin-two fibers	354:383	guar gum and pectin-two fibers with distinct functional characteristics-affect BA profiles, microbiota composition, and gut metabolites in rats	354:496	This study investigated whether guar gum and pectin-two fibers with distinct functional characteristics-affect BA profiles, microbiota composition, and gut metabolites in rats.
27315087	5	59	from	composition	1029:1039	arg1	diet					1093:1096	a high-fat diet	1082:1096	a high-fat diet	1082:1096	Strong correlations were observed between cecal BA and SCFA levels, microbiota composition, and portal plasma LBP levels in rats on a high-fat diet.
27315087	5	59	from	composition	1029:1039	arg1	rats					1074:1077	rats	1074:1077	rats on a high-fat diet	1074:1096	Strong correlations were observed between cecal BA and SCFA levels, microbiota composition, and portal plasma LBP levels in rats on a high-fat diet.
27315087	0	60	theme	Gum	29:31	arg1	Properties					10:19	Molecular Properties	0:19	Molecular Properties of Guar Gum and Pectin	0:42	Molecular Properties of Guar Gum and Pectin Modify Cecal Bile Acids, Microbiota, and Plasma Lipopolysaccharide-Binding Protein in Rats.
27315087	9	61	from	family	1921:1926	arg1	RF32					1931:1934	RF32	1931:1934	RF32	1931:1934	The inflammatory marker LBP was downregulated in rats fed medium-MW guar gum and HM pectin; these two fibers decreased the cecal abundance of Oscillospira and an unclassified genus in Ruminococcaceae, and increased that of an unclassified family in RF32.
27315087	4	62	theme	gene	902:905	arg1	sequencing					907:916	16S rRNA gene sequencing	893:916	16S rRNA gene sequencing	893:916	Cecal BAs, short-chain fatty acids (SCFA) and microbiota composition, and plasma lipopolysaccharide-binding protein (LBP) levels were analyzed, by using novel methodologies based on gas chromatography (BAs and SCFAs) and 16S rRNA gene sequencing on the Illumina MiSeq platform.
27315087	0	63	theme	Pectin	37:42	arg1	Properties					10:19	Molecular Properties	0:19	Molecular Properties of Guar Gum and Pectin	0:42	Molecular Properties of Guar Gum and Pectin Modify Cecal Bile Acids, Microbiota, and Plasma Lipopolysaccharide-Binding Protein in Rats.
27315087	5	64	theme	portal	1046:1051	arg1	levels					1064:1069	portal plasma LBP levels	1046:1069	portal plasma LBP levels	1046:1069	Strong correlations were observed between cecal BA and SCFA levels, microbiota composition, and portal plasma LBP levels in rats on a high-fat diet.
27315087	6	65	theme	fiber-free	1327:1336	arg1	diet					1346:1349	the fiber-free control diet	1323:1349	the fiber-free control diet	1323:1349	Notably, guar gum consumption with medium-MW increased the cecal amounts of cholic-, chenodeoxycholic-, and ursodeoxycholic acids as well as α-, β-, and ω-muricholic acids to a greater extent than other types of guar gum or the fiber-free control diet.
27315087	1	66	theme	physiological	191:203	arg1	processes					205:213	various physiological processes	183:213	various physiological processes	183:213	Bile acids (BAs) act as signaling molecules in various physiological processes, and are related to colonic microbiota composition as well as to different types of dietary fat and fiber.
27315087	5	67	from	levels	1010:1015	arg1	diet					1093:1096	a high-fat diet	1082:1096	a high-fat diet	1082:1096	Strong correlations were observed between cecal BA and SCFA levels, microbiota composition, and portal plasma LBP levels in rats on a high-fat diet.
27315087	5	67	from	levels	1010:1015	arg1	rats					1074:1077	rats	1074:1077	rats on a high-fat diet	1074:1096	Strong correlations were observed between cecal BA and SCFA levels, microbiota composition, and portal plasma LBP levels in rats on a high-fat diet.
27315087	9	68	from	abundance	1811:1819	arg1	Ruminococcaceae					1866:1880	Ruminococcaceae	1866:1880	Ruminococcaceae	1866:1880	The inflammatory marker LBP was downregulated in rats fed medium-MW guar gum and HM pectin; these two fibers decreased the cecal abundance of Oscillospira and an unclassified genus in Ruminococcaceae, and increased that of an unclassified family in RF32.
27315087	6	69	theme	guar	1311:1314	arg1	gum					1316:1318	guar gum	1311:1318	guar gum	1311:1318	Notably, guar gum consumption with medium-MW increased the cecal amounts of cholic-, chenodeoxycholic-, and ursodeoxycholic acids as well as α-, β-, and ω-muricholic acids to a greater extent than other types of guar gum or the fiber-free control diet.
27315087	10	70	theme	cecal	2057:2061	arg1	formation					2066:2074	cecal BA formation	2057:2074	cecal BA formation	2057:2074	These results indicate that the molecular properties of guar gum and pectin are important for their ability to modulate cecal BA formation, gut microbiota composition, and high-fat diet induced inflammation.
27315087	9	71	theme	inflammatory	1686:1697	arg1	LBP					1706:1708	The inflammatory marker LBP	1682:1708	The inflammatory marker LBP	1682:1708	The inflammatory marker LBP was downregulated in rats fed medium-MW guar gum and HM pectin; these two fibers decreased the cecal abundance of Oscillospira and an unclassified genus in Ruminococcaceae, and increased that of an unclassified family in RF32.
27315087	5	72	theme	high-fat	1084:1091	arg1	diet					1093:1096	a high-fat diet	1082:1096	a high-fat diet	1082:1096	Strong correlations were observed between cecal BA and SCFA levels, microbiota composition, and portal plasma LBP levels in rats on a high-fat diet.
27315087	6	73	theme	ω-muricholic	1252:1263	arg1	acids					1265:1269	α-, β-, and ω-muricholic acids	1240:1269	cholic-, chenodeoxycholic-, and ursodeoxycholic acids as well as α-, β-, and ω-muricholic acids	1175:1269	Notably, guar gum consumption with medium-MW increased the cecal amounts of cholic-, chenodeoxycholic-, and ursodeoxycholic acids as well as α-, β-, and ω-muricholic acids to a greater extent than other types of guar gum or the fiber-free control diet.
27315087	10	74	theme	gut	2077:2079	arg1	composition					2092:2102	gut microbiota composition	2077:2102	gut microbiota composition	2077:2102	These results indicate that the molecular properties of guar gum and pectin are important for their ability to modulate cecal BA formation, gut microbiota composition, and high-fat diet induced inflammation.
27315087	1	75	theme	different	280:288	arg1	types					290:294	different types	280:294	different types of dietary fat and fiber	280:319	Bile acids (BAs) act as signaling molecules in various physiological processes, and are related to colonic microbiota composition as well as to different types of dietary fat and fiber.
27315087	5	76	theme	microbiota	1018:1027	arg1	composition					1029:1039	microbiota composition	1018:1039	microbiota composition	1018:1039	Strong correlations were observed between cecal BA and SCFA levels, microbiota composition, and portal plasma LBP levels in rats on a high-fat diet.
27315087	6	77	theme	gum	1113:1115	arg1	consumption					1117:1127	guar gum consumption	1108:1127	guar gum consumption with medium-MW	1108:1142	Notably, guar gum consumption with medium-MW increased the cecal amounts of cholic-, chenodeoxycholic-, and ursodeoxycholic acids as well as α-, β-, and ω-muricholic acids to a greater extent than other types of guar gum or the fiber-free control diet.
27315087	6	78	theme	acids	1223:1227	arg1	acids					1223:1227	cholic-, chenodeoxycholic-, and ursodeoxycholic acids	1175:1227	cholic-, chenodeoxycholic-, and ursodeoxycholic acids as well as α-, β-, and ω-muricholic acids	1175:1269	Notably, guar gum consumption with medium-MW increased the cecal amounts of cholic-, chenodeoxycholic-, and ursodeoxycholic acids as well as α-, β-, and ω-muricholic acids to a greater extent than other types of guar gum or the fiber-free control diet.
27315087	6	78	theme	acids	1223:1227	arg1	acids					1265:1269	α-, β-, and ω-muricholic acids	1240:1269	cholic-, chenodeoxycholic-, and ursodeoxycholic acids as well as α-, β-, and ω-muricholic acids	1175:1269	Notably, guar gum consumption with medium-MW increased the cecal amounts of cholic-, chenodeoxycholic-, and ursodeoxycholic acids as well as α-, β-, and ω-muricholic acids to a greater extent than other types of guar gum or the fiber-free control diet.
27315087	6	78	theme	acids	1223:1227	arg1	amounts					1164:1170	the cecal amounts	1154:1170	the cecal amounts of cholic-, chenodeoxycholic-, and ursodeoxycholic acids as well as α-, β-, and ω-muricholic acids	1154:1269	Notably, guar gum consumption with medium-MW increased the cecal amounts of cholic-, chenodeoxycholic-, and ursodeoxycholic acids as well as α-, β-, and ω-muricholic acids to a greater extent than other types of guar gum or the fiber-free control diet.
27315087	3	79	theme	high-methoxylated	512:528	arg1	pectin					535:540	Low- (LM) or high-methoxylated (HM) pectin	499:540	Low- (LM) or high-methoxylated (HM) pectin	499:540	Low- (LM) or high-methoxylated (HM) pectin, and low-, medium-, or high-molecular-weight (MW) guar gum were administered to rats that were fed either low- or high-fat diets.
27315087	1	80	theme	signaling	160:168	arg1	molecules					170:178	signaling molecules	160:178	signaling molecules in various physiological processes	160:213	Bile acids (BAs) act as signaling molecules in various physiological processes, and are related to colonic microbiota composition as well as to different types of dietary fat and fiber.
27315087	1	80	theme	signaling	160:168	arg1	acids					141:145	Bile acids	136:145	Bile acids (BAs)	136:151	Bile acids (BAs) act as signaling molecules in various physiological processes, and are related to colonic microbiota composition as well as to different types of dietary fat and fiber.
27315087	7	81	theme	gum	1459:1461	arg1	types					1445:1449	all types	1441:1449	all types of guar gum independent of chain length	1441:1489	In contrast, the amounts of cecal deoxycholic- and hyodeoxycholic acid were reduced with all types of guar gum independent of chain length.
27315087	9	82	theme	medium-MW	1740:1748	arg1	gum					1755:1757	medium-MW guar gum	1740:1757	medium-MW guar gum	1740:1757	The inflammatory marker LBP was downregulated in rats fed medium-MW guar gum and HM pectin; these two fibers decreased the cecal abundance of Oscillospira and an unclassified genus in Ruminococcaceae, and increased that of an unclassified family in RF32.
27315087	6	83	theme	diet	1346:1349	arg1	types					1302:1306	other types	1296:1306	other types of guar gum or the fiber-free control diet	1296:1349	Notably, guar gum consumption with medium-MW increased the cecal amounts of cholic-, chenodeoxycholic-, and ursodeoxycholic acids as well as α-, β-, and ω-muricholic acids to a greater extent than other types of guar gum or the fiber-free control diet.
27315087	2	84	from	metabolites	478:488	arg1	rats					493:496	rats	493:496	rats	493:496	This study investigated whether guar gum and pectin-two fibers with distinct functional characteristics-affect BA profiles, microbiota composition, and gut metabolites in rats.
27315087	6	85	theme	chenodeoxycholic-	1184:1200	arg1	acids					1223:1227	cholic-, chenodeoxycholic-, and ursodeoxycholic acids	1175:1227	cholic-, chenodeoxycholic-, and ursodeoxycholic acids as well as α-, β-, and ω-muricholic acids	1175:1269	Notably, guar gum consumption with medium-MW increased the cecal amounts of cholic-, chenodeoxycholic-, and ursodeoxycholic acids as well as α-, β-, and ω-muricholic acids to a greater extent than other types of guar gum or the fiber-free control diet.
27315087	10	86	theme	high-fat	2109:2116	arg1	diet					2118:2121	high-fat diet	2109:2121	high-fat diet induced inflammation	2109:2142	These results indicate that the molecular properties of guar gum and pectin are important for their ability to modulate cecal BA formation, gut microbiota composition, and high-fat diet induced inflammation.
27315087	9	87	theme	HM	1763:1764	arg1	pectin					1766:1771	HM pectin	1763:1771	HM pectin	1763:1771	The inflammatory marker LBP was downregulated in rats fed medium-MW guar gum and HM pectin; these two fibers decreased the cecal abundance of Oscillospira and an unclassified genus in Ruminococcaceae, and increased that of an unclassified family in RF32.
27315087	6	88	theme	cholic-	1175:1181	arg1	acids					1223:1227	cholic-, chenodeoxycholic-, and ursodeoxycholic acids	1175:1227	cholic-, chenodeoxycholic-, and ursodeoxycholic acids as well as α-, β-, and ω-muricholic acids	1175:1269	Notably, guar gum consumption with medium-MW increased the cecal amounts of cholic-, chenodeoxycholic-, and ursodeoxycholic acids as well as α-, β-, and ω-muricholic acids to a greater extent than other types of guar gum or the fiber-free control diet.
27315087	1	89	from	molecules	170:178	arg1	processes					205:213	various physiological processes	183:213	various physiological processes	183:213	Bile acids (BAs) act as signaling molecules in various physiological processes, and are related to colonic microbiota composition as well as to different types of dietary fat and fiber.
27315087	10	90	theme	guar	1993:1996	arg1	gum					1998:2000	guar gum	1993:2000	guar gum	1993:2000	These results indicate that the molecular properties of guar gum and pectin are important for their ability to modulate cecal BA formation, gut microbiota composition, and high-fat diet induced inflammation.
27315087	0	91	theme	Bile	57:60	arg1	Acids					62:66	Cecal Bile Acids	51:66	Cecal Bile Acids	51:66	Molecular Properties of Guar Gum and Pectin Modify Cecal Bile Acids, Microbiota, and Plasma Lipopolysaccharide-Binding Protein in Rats.
27315087	5	92	theme	Strong	950:955	arg1	correlations					957:968	Strong correlations	950:968	Strong correlations	950:968	Strong correlations were observed between cecal BA and SCFA levels, microbiota composition, and portal plasma LBP levels in rats on a high-fat diet.
27315087	8	93	theme	acids	1603:1607	arg1	levels					1573:1578	cecal levels	1567:1578	cecal levels of α- and ω-muricholic acids	1567:1607	Differences in BA composition between pectin groups were less obvious, but cecal levels of α- and ω-muricholic acids were higher in rats fed LM as compared to HM pectin or the control diet.
27315087	3	94	dep	high-methoxylated	512:528	arg1	HM					531:532	HM	531:532	HM	531:532	Low- (LM) or high-methoxylated (HM) pectin, and low-, medium-, or high-molecular-weight (MW) guar gum were administered to rats that were fed either low- or high-fat diets.
27315087	7	95	theme	acid	1418:1421	arg1	amounts					1369:1375	the amounts	1365:1375	the amounts of cecal deoxycholic- and hyodeoxycholic acid	1365:1421	In contrast, the amounts of cecal deoxycholic- and hyodeoxycholic acid were reduced with all types of guar gum independent of chain length.
27315087	7	95	theme	acid	1418:1421	arg1	deoxycholic-					1386:1397	cecal deoxycholic-	1380:1397	cecal deoxycholic-	1380:1397	In contrast, the amounts of cecal deoxycholic- and hyodeoxycholic acid were reduced with all types of guar gum independent of chain length.
27315087	7	95	theme	acid	1418:1421	arg1	acid					1418:1421	hyodeoxycholic acid	1403:1421	hyodeoxycholic acid	1403:1421	In contrast, the amounts of cecal deoxycholic- and hyodeoxycholic acid were reduced with all types of guar gum independent of chain length.
27315087	2	96	theme	gut	474:476	arg1	metabolites					478:488	gut metabolites	474:488	gut metabolites	474:488	This study investigated whether guar gum and pectin-two fibers with distinct functional characteristics-affect BA profiles, microbiota composition, and gut metabolites in rats.
27315087	6	97	theme	β-	1244:1245	arg1	acids					1265:1269	α-, β-, and ω-muricholic acids	1240:1269	cholic-, chenodeoxycholic-, and ursodeoxycholic acids as well as α-, β-, and ω-muricholic acids	1175:1269	Notably, guar gum consumption with medium-MW increased the cecal amounts of cholic-, chenodeoxycholic-, and ursodeoxycholic acids as well as α-, β-, and ω-muricholic acids to a greater extent than other types of guar gum or the fiber-free control diet.
27315087	1	98	theme	dietary	299:305	arg1	fat					307:309	dietary fat	299:309	dietary fat	299:309	Bile acids (BAs) act as signaling molecules in various physiological processes, and are related to colonic microbiota composition as well as to different types of dietary fat and fiber.
27315087	5	99	theme	cecal	992:996	arg1	BA					998:999	cecal BA	992:999	cecal BA	992:999	Strong correlations were observed between cecal BA and SCFA levels, microbiota composition, and portal plasma LBP levels in rats on a high-fat diet.
27315087	5	100	from	diet	1093:1096	arg1	levels					1064:1069	portal plasma LBP levels	1046:1069	portal plasma LBP levels	1046:1069	Strong correlations were observed between cecal BA and SCFA levels, microbiota composition, and portal plasma LBP levels in rats on a high-fat diet.
27315087	5	100	from	diet	1093:1096	arg1	levels					1010:1015	cecal BA and SCFA levels	992:1015	cecal BA and SCFA levels	992:1015	Strong correlations were observed between cecal BA and SCFA levels, microbiota composition, and portal plasma LBP levels in rats on a high-fat diet.
27315087	5	100	from	diet	1093:1096	arg1	composition					1029:1039	microbiota composition	1018:1039	microbiota composition	1018:1039	Strong correlations were observed between cecal BA and SCFA levels, microbiota composition, and portal plasma LBP levels in rats on a high-fat diet.
27315087	6	101	theme	α-	1240:1241	arg1	acids					1265:1269	α-, β-, and ω-muricholic acids	1240:1269	cholic-, chenodeoxycholic-, and ursodeoxycholic acids as well as α-, β-, and ω-muricholic acids	1175:1269	Notably, guar gum consumption with medium-MW increased the cecal amounts of cholic-, chenodeoxycholic-, and ursodeoxycholic acids as well as α-, β-, and ω-muricholic acids to a greater extent than other types of guar gum or the fiber-free control diet.
27315087	7	102	theme	cecal	1380:1384	arg1	deoxycholic-					1386:1397	cecal deoxycholic-	1380:1397	cecal deoxycholic-	1380:1397	In contrast, the amounts of cecal deoxycholic- and hyodeoxycholic acid were reduced with all types of guar gum independent of chain length.
27315087	9	103	theme	cecal	1805:1809	arg1	abundance					1811:1819	the cecal abundance	1801:1819	the cecal abundance of Oscillospira	1801:1835	The inflammatory marker LBP was downregulated in rats fed medium-MW guar gum and HM pectin; these two fibers decreased the cecal abundance of Oscillospira and an unclassified genus in Ruminococcaceae, and increased that of an unclassified family in RF32.
27315087	8	104	from	Differences	1492:1502	arg1	composition					1510:1520	BA composition	1507:1520	BA composition	1507:1520	Differences in BA composition between pectin groups were less obvious, but cecal levels of α- and ω-muricholic acids were higher in rats fed LM as compared to HM pectin or the control diet.
27315087	0	105	theme	Plasma	85:90	arg1	Protein					119:125	Plasma Lipopolysaccharide-Binding Protein	85:125	Plasma Lipopolysaccharide-Binding Protein in Rats	85:133	Molecular Properties of Guar Gum and Pectin Modify Cecal Bile Acids, Microbiota, and Plasma Lipopolysaccharide-Binding Protein in Rats.
27315087	5	106	theme	BA	998:999	arg1	levels					1010:1015	cecal BA and SCFA levels	992:1015	cecal BA and SCFA levels	992:1015	Strong correlations were observed between cecal BA and SCFA levels, microbiota composition, and portal plasma LBP levels in rats on a high-fat diet.
27315087	2	107	from	composition	457:467	arg1	rats					493:496	rats	493:496	rats	493:496	This study investigated whether guar gum and pectin-two fibers with distinct functional characteristics-affect BA profiles, microbiota composition, and gut metabolites in rats.
27315087	4	108	theme	short-chain	683:693	arg1	SCFA					708:711	SCFA	708:711	SCFA	708:711	Cecal BAs, short-chain fatty acids (SCFA) and microbiota composition, and plasma lipopolysaccharide-binding protein (LBP) levels were analyzed, by using novel methodologies based on gas chromatography (BAs and SCFAs) and 16S rRNA gene sequencing on the Illumina MiSeq platform.
27315087	4	108	theme	short-chain	683:693	arg1	acids					701:705	short-chain fatty acids	683:705	short-chain fatty acids (SCFA)	683:712	Cecal BAs, short-chain fatty acids (SCFA) and microbiota composition, and plasma lipopolysaccharide-binding protein (LBP) levels were analyzed, by using novel methodologies based on gas chromatography (BAs and SCFAs) and 16S rRNA gene sequencing on the Illumina MiSeq platform.
27315087	2	109	theme	guar	354:357	arg1	gum					359:361	guar gum	354:361	guar gum	354:361	This study investigated whether guar gum and pectin-two fibers with distinct functional characteristics-affect BA profiles, microbiota composition, and gut metabolites in rats.
27315087	6	110	theme	other	1296:1300	arg1	types					1302:1306	other types	1296:1306	other types of guar gum or the fiber-free control diet	1296:1349	Notably, guar gum consumption with medium-MW increased the cecal amounts of cholic-, chenodeoxycholic-, and ursodeoxycholic acids as well as α-, β-, and ω-muricholic acids to a greater extent than other types of guar gum or the fiber-free control diet.
27315087	5	111	theme	SCFA	1005:1008	arg1	levels					1010:1015	cecal BA and SCFA levels	992:1015	cecal BA and SCFA levels	992:1015	Strong correlations were observed between cecal BA and SCFA levels, microbiota composition, and portal plasma LBP levels in rats on a high-fat diet.
27315087	2	112	theme	characteristics-affect	410:431	arg1	profiles					436:443	distinct functional characteristics-affect BA profiles	390:443	distinct functional characteristics-affect BA profiles	390:443	This study investigated whether guar gum and pectin-two fibers with distinct functional characteristics-affect BA profiles, microbiota composition, and gut metabolites in rats.
27315087	0	113	theme	Guar	24:27	arg1	Gum					29:31	Guar Gum	24:31	Guar Gum	24:31	Molecular Properties of Guar Gum and Pectin Modify Cecal Bile Acids, Microbiota, and Plasma Lipopolysaccharide-Binding Protein in Rats.
27315087	8	114	theme	HM	1651:1652	arg1	pectin					1654:1659	HM pectin	1651:1659	HM pectin	1651:1659	Differences in BA composition between pectin groups were less obvious, but cecal levels of α- and ω-muricholic acids were higher in rats fed LM as compared to HM pectin or the control diet.
27315087	2	115	theme	distinct	390:397	arg1	profiles					436:443	distinct functional characteristics-affect BA profiles	390:443	distinct functional characteristics-affect BA profiles	390:443	This study investigated whether guar gum and pectin-two fibers with distinct functional characteristics-affect BA profiles, microbiota composition, and gut metabolites in rats.
27315087	9	116	theme	unclassified	1844:1855	arg1	genus					1857:1861	an unclassified genus	1841:1861	an unclassified genus in Ruminococcaceae	1841:1880	The inflammatory marker LBP was downregulated in rats fed medium-MW guar gum and HM pectin; these two fibers decreased the cecal abundance of Oscillospira and an unclassified genus in Ruminococcaceae, and increased that of an unclassified family in RF32.
27315087	8	117	theme	cecal	1567:1571	arg1	levels					1573:1578	cecal levels	1567:1578	cecal levels of α- and ω-muricholic acids	1567:1607	Differences in BA composition between pectin groups were less obvious, but cecal levels of α- and ω-muricholic acids were higher in rats fed LM as compared to HM pectin or the control diet.
27315087	2	118	theme	microbiota	446:455	arg1	composition					457:467	microbiota composition	446:467	microbiota composition	446:467	This study investigated whether guar gum and pectin-two fibers with distinct functional characteristics-affect BA profiles, microbiota composition, and gut metabolites in rats.
27315087	3	119	theme	high-molecular-weight	565:585	arg1	MW					588:589	MW	588:589	MW	588:589	Low- (LM) or high-methoxylated (HM) pectin, and low-, medium-, or high-molecular-weight (MW) guar gum were administered to rats that were fed either low- or high-fat diets.
27315087	3	119	theme	high-molecular-weight	565:585	arg1	gum					597:599	low-, medium-, or high-molecular-weight (MW) guar gum	547:599	low-, medium-, or high-molecular-weight (MW) guar gum	547:599	Low- (LM) or high-methoxylated (HM) pectin, and low-, medium-, or high-molecular-weight (MW) guar gum were administered to rats that were fed either low- or high-fat diets.
27315087	6	120	theme	acids	1265:1269	arg1	acids					1223:1227	cholic-, chenodeoxycholic-, and ursodeoxycholic acids	1175:1227	cholic-, chenodeoxycholic-, and ursodeoxycholic acids as well as α-, β-, and ω-muricholic acids	1175:1269	Notably, guar gum consumption with medium-MW increased the cecal amounts of cholic-, chenodeoxycholic-, and ursodeoxycholic acids as well as α-, β-, and ω-muricholic acids to a greater extent than other types of guar gum or the fiber-free control diet.
27315087	6	120	theme	acids	1265:1269	arg1	acids					1265:1269	α-, β-, and ω-muricholic acids	1240:1269	cholic-, chenodeoxycholic-, and ursodeoxycholic acids as well as α-, β-, and ω-muricholic acids	1175:1269	Notably, guar gum consumption with medium-MW increased the cecal amounts of cholic-, chenodeoxycholic-, and ursodeoxycholic acids as well as α-, β-, and ω-muricholic acids to a greater extent than other types of guar gum or the fiber-free control diet.
27315087	6	120	theme	acids	1265:1269	arg1	amounts					1164:1170	the cecal amounts	1154:1170	the cecal amounts of cholic-, chenodeoxycholic-, and ursodeoxycholic acids as well as α-, β-, and ω-muricholic acids	1154:1269	Notably, guar gum consumption with medium-MW increased the cecal amounts of cholic-, chenodeoxycholic-, and ursodeoxycholic acids as well as α-, β-, and ω-muricholic acids to a greater extent than other types of guar gum or the fiber-free control diet.
27315087	4	121	theme	lipopolysaccharide-binding	753:778	arg1	LBP					789:791	LBP	789:791	LBP	789:791	Cecal BAs, short-chain fatty acids (SCFA) and microbiota composition, and plasma lipopolysaccharide-binding protein (LBP) levels were analyzed, by using novel methodologies based on gas chromatography (BAs and SCFAs) and 16S rRNA gene sequencing on the Illumina MiSeq platform.
27315087	4	121	theme	lipopolysaccharide-binding	753:778	arg1	protein					780:786	plasma lipopolysaccharide-binding protein	746:786	plasma lipopolysaccharide-binding protein (LBP) levels	746:799	Cecal BAs, short-chain fatty acids (SCFA) and microbiota composition, and plasma lipopolysaccharide-binding protein (LBP) levels were analyzed, by using novel methodologies based on gas chromatography (BAs and SCFAs) and 16S rRNA gene sequencing on the Illumina MiSeq platform.
27315087	4	122	theme	rRNA	897:900	arg1	sequencing					907:916	16S rRNA gene sequencing	893:916	16S rRNA gene sequencing	893:916	Cecal BAs, short-chain fatty acids (SCFA) and microbiota composition, and plasma lipopolysaccharide-binding protein (LBP) levels were analyzed, by using novel methodologies based on gas chromatography (BAs and SCFAs) and 16S rRNA gene sequencing on the Illumina MiSeq platform.
27315087	6	123	theme	control	1338:1344	arg1	diet					1346:1349	the fiber-free control diet	1323:1349	the fiber-free control diet	1323:1349	Notably, guar gum consumption with medium-MW increased the cecal amounts of cholic-, chenodeoxycholic-, and ursodeoxycholic acids as well as α-, β-, and ω-muricholic acids to a greater extent than other types of guar gum or the fiber-free control diet.
27315087	5	124	theme	plasma	1053:1058	arg1	levels					1064:1069	portal plasma LBP levels	1046:1069	portal plasma LBP levels	1046:1069	Strong correlations were observed between cecal BA and SCFA levels, microbiota composition, and portal plasma LBP levels in rats on a high-fat diet.
27315087	3	125	theme	low-	547:550	arg1	MW					588:589	MW	588:589	MW	588:589	Low- (LM) or high-methoxylated (HM) pectin, and low-, medium-, or high-molecular-weight (MW) guar gum were administered to rats that were fed either low- or high-fat diets.
27315087	3	125	theme	low-	547:550	arg1	gum					597:599	low-, medium-, or high-molecular-weight (MW) guar gum	547:599	low-, medium-, or high-molecular-weight (MW) guar gum	547:599	Low- (LM) or high-methoxylated (HM) pectin, and low-, medium-, or high-molecular-weight (MW) guar gum were administered to rats that were fed either low- or high-fat diets.
27315087	3	126	theme	guar	592:595	arg1	MW					588:589	MW	588:589	MW	588:589	Low- (LM) or high-methoxylated (HM) pectin, and low-, medium-, or high-molecular-weight (MW) guar gum were administered to rats that were fed either low- or high-fat diets.
27315087	3	126	theme	guar	592:595	arg1	gum					597:599	low-, medium-, or high-molecular-weight (MW) guar gum	547:599	low-, medium-, or high-molecular-weight (MW) guar gum	547:599	Low- (LM) or high-methoxylated (HM) pectin, and low-, medium-, or high-molecular-weight (MW) guar gum were administered to rats that were fed either low- or high-fat diets.
27315087	2	127	dep	investigated	333:344	arg1	fibers					378:383	guar gum and pectin-two fibers	354:383	guar gum and pectin-two fibers with distinct functional characteristics-affect BA profiles, microbiota composition, and gut metabolites in rats	354:496	This study investigated whether guar gum and pectin-two fibers with distinct functional characteristics-affect BA profiles, microbiota composition, and gut metabolites in rats.
27315087	6	128	with	consumption	1117:1127	arg1	medium-MW					1134:1142	medium-MW	1134:1142	medium-MW	1134:1142	Notably, guar gum consumption with medium-MW increased the cecal amounts of cholic-, chenodeoxycholic-, and ursodeoxycholic acids as well as α-, β-, and ω-muricholic acids to a greater extent than other types of guar gum or the fiber-free control diet.
27315087	0	129	theme	Lipopolysaccharide-Binding	92:117	arg1	Protein					119:125	Plasma Lipopolysaccharide-Binding Protein	85:125	Plasma Lipopolysaccharide-Binding Protein in Rats	85:133	Molecular Properties of Guar Gum and Pectin Modify Cecal Bile Acids, Microbiota, and Plasma Lipopolysaccharide-Binding Protein in Rats.
27315087	0	130	from	Protein	119:125	arg1	Rats					130:133	Rats	130:133	Rats	130:133	Molecular Properties of Guar Gum and Pectin Modify Cecal Bile Acids, Microbiota, and Plasma Lipopolysaccharide-Binding Protein in Rats.
27315087	3	131	theme	Low-	499:502	arg1	pectin					535:540	Low- (LM) or high-methoxylated (HM) pectin	499:540	Low- (LM) or high-methoxylated (HM) pectin	499:540	Low- (LM) or high-methoxylated (HM) pectin, and low-, medium-, or high-molecular-weight (MW) guar gum were administered to rats that were fed either low- or high-fat diets.
27315087	6	132	theme	gum	1316:1318	arg1	types					1302:1306	other types	1296:1306	other types of guar gum or the fiber-free control diet	1296:1349	Notably, guar gum consumption with medium-MW increased the cecal amounts of cholic-, chenodeoxycholic-, and ursodeoxycholic acids as well as α-, β-, and ω-muricholic acids to a greater extent than other types of guar gum or the fiber-free control diet.
26275826	2	0	theme	combined	377:384	arg1	process					386:392	a combined process	375:392	a combined process consisting of lime treatment, resin adsorption, and gel filtration	375:459	In this study, a combined process consisting of lime treatment, resin adsorption, and gel filtration was developed to separate HDSs from NSCs.
26275826	6	1	with	xylooligosaccharides	908:927	arg1	degree					944:949	the degree	940:949	the degree of depolymerization from 2 to 6	940:981	Chemical composition analysis showed that xylooligosaccharides (XOS) with the degree of depolymerization from 2 to 6 accounted for 28% of the total purified HDSs.
26275826	0	2	theme	combined	90:97	arg1	process					99:105	combined process	90:105	combined process	90:105	Separation and purification of hemicellulose-derived saccharides from wood hydrolysate by combined process.
26275826	6	3	dep	6	981:981	arg1	to					978:979	to	978:979	to	978:979	Chemical composition analysis showed that xylooligosaccharides (XOS) with the degree of depolymerization from 2 to 6 accounted for 28% of the total purified HDSs.
26275826	0	4	from	hydrolysate	75:85	arg1	purification					15:26	purification	15:26	purification	15:26	Separation and purification of hemicellulose-derived saccharides from wood hydrolysate by combined process.
26275826	0	4	from	hydrolysate	75:85	arg1	Separation					0:9	Separation	0:9	Separation	0:9	Separation and purification of hemicellulose-derived saccharides from wood hydrolysate by combined process.
26275826	6	5	theme	composition	875:885	arg1	analysis					887:894	Chemical composition analysis	866:894	Chemical composition analysis	866:894	Chemical composition analysis showed that xylooligosaccharides (XOS) with the degree of depolymerization from 2 to 6 accounted for 28% of the total purified HDSs.
26275826	4	6	theme	NSCs	744:747	arg1	removal					749:755	NSCs removal	744:755	NSCs removal	744:755	The majority of NSCs, lignin-derived phenolics, were eliminated by mixed bed ion exchange resin, elevating NSCs removal to 94.0%.
26275826	3	7	theme	NSCs	559:562	arg1	NSCs					559:562	NSCs	559:562	NSCs	559:562	The macro-lignin impurities that accounted for 32.2% of NSCs were removed by lime treatment at 1.2% dosage with negligible HDSs loss.
26275826	3	7	theme	NSCs	559:562	arg1	%					554:554	32.2%	550:554	32.2% of NSCs	550:562	The macro-lignin impurities that accounted for 32.2% of NSCs were removed by lime treatment at 1.2% dosage with negligible HDSs loss.
26275826	4	8	theme	bed	710:712	arg1	resin					727:731	mixed bed ion exchange resin	704:731	mixed bed ion exchange resin	704:731	The majority of NSCs, lignin-derived phenolics, were eliminated by mixed bed ion exchange resin, elevating NSCs removal to 94.0%.
26275826	4	9	theme	mixed	704:708	arg1	resin					727:731	mixed bed ion exchange resin	704:731	mixed bed ion exchange resin	704:731	The majority of NSCs, lignin-derived phenolics, were eliminated by mixed bed ion exchange resin, elevating NSCs removal to 94.0%.
26275826	4	10	theme	lignin-derived	659:672	arg1	NSCs					653:656	NSCs	653:656	NSCs	653:656	The majority of NSCs, lignin-derived phenolics, were eliminated by mixed bed ion exchange resin, elevating NSCs removal to 94.0%.
26275826	4	10	theme	lignin-derived	659:672	arg1	phenolics					674:682	lignin-derived phenolics	659:682	lignin-derived phenolics	659:682	The majority of NSCs, lignin-derived phenolics, were eliminated by mixed bed ion exchange resin, elevating NSCs removal to 94.0%.
26275826	4	11	theme	NSCs	653:656	arg1	majority					641:648	The majority	637:648	The majority of NSCs, lignin-derived phenolics,	637:683	The majority of NSCs, lignin-derived phenolics, were eliminated by mixed bed ion exchange resin, elevating NSCs removal to 94.0%.
26275826	1	12	theme	hemicellulose-derived	190:210	arg1	saccharides					212:222	hemicellulose-derived saccharides	190:222	hemicellulose-derived saccharides (HDSs) but also undesired non-saccharide compounds (NSCs)	190:280	Prehydrolysis of wood biomass prior to kraft cooking provides a stream containing hemicellulose-derived saccharides (HDSs) but also undesired non-saccharide compounds (NSCs) that were resulted from lignin depolymerization and carbohydrate degradation.
26275826	1	12	theme	hemicellulose-derived	190:210	arg1	HDSs					225:228	HDSs	225:228	HDSs	225:228	Prehydrolysis of wood biomass prior to kraft cooking provides a stream containing hemicellulose-derived saccharides (HDSs) but also undesired non-saccharide compounds (NSCs) that were resulted from lignin depolymerization and carbohydrate degradation.
26275826	6	13	from	6	981:981	arg1	degree					944:949	the degree	940:949	the degree of depolymerization from 2 to 6	940:981	Chemical composition analysis showed that xylooligosaccharides (XOS) with the degree of depolymerization from 2 to 6 accounted for 28% of the total purified HDSs.
26275826	6	13	from	6	981:981	arg1	depolymerization					954:969	depolymerization	954:969	depolymerization from 2 to 6	954:981	Chemical composition analysis showed that xylooligosaccharides (XOS) with the degree of depolymerization from 2 to 6 accounted for 28% of the total purified HDSs.
26275826	6	14	theme	Chemical	866:873	arg1	analysis					887:894	Chemical composition analysis	866:894	Chemical composition analysis	866:894	Chemical composition analysis showed that xylooligosaccharides (XOS) with the degree of depolymerization from 2 to 6 accounted for 28% of the total purified HDSs.
26275826	2	15	theme	lime	408:411	arg1	treatment					413:421	lime treatment	408:421	lime treatment	408:421	In this study, a combined process consisting of lime treatment, resin adsorption, and gel filtration was developed to separate HDSs from NSCs.
26275826	6	16	theme	depolymerization	954:969	arg1	degree					944:949	the degree	940:949	the degree of depolymerization from 2 to 6	940:981	Chemical composition analysis showed that xylooligosaccharides (XOS) with the degree of depolymerization from 2 to 6 accounted for 28% of the total purified HDSs.
26275826	1	17	link	hemicellulose-derived	190:210	arg1	saccharides					212:222	hemicellulose-derived saccharides	190:222	hemicellulose-derived saccharides (HDSs) but also undesired non-saccharide compounds (NSCs)	190:280	Prehydrolysis of wood biomass prior to kraft cooking provides a stream containing hemicellulose-derived saccharides (HDSs) but also undesired non-saccharide compounds (NSCs) that were resulted from lignin depolymerization and carbohydrate degradation.
26275826	1	17	link	hemicellulose-derived	190:210	arg1	HDSs					225:228	HDSs	225:228	HDSs	225:228	Prehydrolysis of wood biomass prior to kraft cooking provides a stream containing hemicellulose-derived saccharides (HDSs) but also undesired non-saccharide compounds (NSCs) that were resulted from lignin depolymerization and carbohydrate degradation.
26275826	3	18	theme	lime	580:583	arg1	treatment					585:593	lime treatment	580:593	lime treatment	580:593	The macro-lignin impurities that accounted for 32.2% of NSCs were removed by lime treatment at 1.2% dosage with negligible HDSs loss.
26275826	5	19	theme	gel	850:852	arg1	filtration					854:863	gel filtration	850:863	gel filtration	850:863	The remaining NSCs, furfural and hydroxymethylfurfural, were excluded from HDSs by gel filtration.
26275826	4	20	theme	exchange	718:725	arg1	resin					727:731	mixed bed ion exchange resin	704:731	mixed bed ion exchange resin	704:731	The majority of NSCs, lignin-derived phenolics, were eliminated by mixed bed ion exchange resin, elevating NSCs removal to 94.0%.
26275826	6	21	from	degree	944:949	arg1	6					981:981	6	981:981	6	981:981	Chemical composition analysis showed that xylooligosaccharides (XOS) with the degree of depolymerization from 2 to 6 accounted for 28% of the total purified HDSs.
26275826	0	22	link	hemicellulose-derived	31:51	arg1	saccharides					53:63	hemicellulose-derived saccharides	31:63	hemicellulose-derived saccharides	31:63	Separation and purification of hemicellulose-derived saccharides from wood hydrolysate by combined process.
26275826	4	23	link	lignin-derived	659:672	arg1	NSCs					653:656	NSCs	653:656	NSCs	653:656	The majority of NSCs, lignin-derived phenolics, were eliminated by mixed bed ion exchange resin, elevating NSCs removal to 94.0%.
26275826	4	23	link	lignin-derived	659:672	arg1	phenolics					674:682	lignin-derived phenolics	659:682	lignin-derived phenolics	659:682	The majority of NSCs, lignin-derived phenolics, were eliminated by mixed bed ion exchange resin, elevating NSCs removal to 94.0%.
26275826	6	24	theme	purified	1014:1021	arg1	HDSs					1023:1026	the total purified HDSs	1004:1026	the total purified HDSs	1004:1026	Chemical composition analysis showed that xylooligosaccharides (XOS) with the degree of depolymerization from 2 to 6 accounted for 28% of the total purified HDSs.
26275826	2	25	theme	gel	446:448	arg1	filtration					450:459	gel filtration	446:459	gel filtration	446:459	In this study, a combined process consisting of lime treatment, resin adsorption, and gel filtration was developed to separate HDSs from NSCs.
26275826	1	26	theme	lignin	306:311	arg1	depolymerization					313:328	lignin depolymerization	306:328	lignin depolymerization	306:328	Prehydrolysis of wood biomass prior to kraft cooking provides a stream containing hemicellulose-derived saccharides (HDSs) but also undesired non-saccharide compounds (NSCs) that were resulted from lignin depolymerization and carbohydrate degradation.
26275826	1	27	theme	wood	125:128	arg1	biomass					130:136	wood biomass	125:136	wood biomass	125:136	Prehydrolysis of wood biomass prior to kraft cooking provides a stream containing hemicellulose-derived saccharides (HDSs) but also undesired non-saccharide compounds (NSCs) that were resulted from lignin depolymerization and carbohydrate degradation.
26275826	6	28	theme	total	1008:1012	arg1	HDSs					1023:1026	the total purified HDSs	1004:1026	the total purified HDSs	1004:1026	Chemical composition analysis showed that xylooligosaccharides (XOS) with the degree of depolymerization from 2 to 6 accounted for 28% of the total purified HDSs.
26275826	0	29	theme	saccharides	53:63	arg1	purification					15:26	purification	15:26	purification	15:26	Separation and purification of hemicellulose-derived saccharides from wood hydrolysate by combined process.
26275826	0	29	theme	saccharides	53:63	arg1	Separation					0:9	Separation	0:9	Separation	0:9	Separation and purification of hemicellulose-derived saccharides from wood hydrolysate by combined process.
26275826	2	30	from	NSCs	497:500	arg1	HDSs					487:490	separate HDSs	478:490	separate HDSs from NSCs	478:500	In this study, a combined process consisting of lime treatment, resin adsorption, and gel filtration was developed to separate HDSs from NSCs.
26275826	3	31	theme	1.2	598:600	arg1	%					601:601	%	601:601	%	601:601	The macro-lignin impurities that accounted for 32.2% of NSCs were removed by lime treatment at 1.2% dosage with negligible HDSs loss.
26275826	6	32	theme	HDSs	1023:1026	arg1	HDSs					1023:1026	the total purified HDSs	1004:1026	the total purified HDSs	1004:1026	Chemical composition analysis showed that xylooligosaccharides (XOS) with the degree of depolymerization from 2 to 6 accounted for 28% of the total purified HDSs.
26275826	6	32	theme	HDSs	1023:1026	arg1	%					999:999	28%	997:999	28% of the total purified HDSs	997:1026	Chemical composition analysis showed that xylooligosaccharides (XOS) with the degree of depolymerization from 2 to 6 accounted for 28% of the total purified HDSs.
26275826	0	33	theme	hemicellulose-derived	31:51	arg1	saccharides					53:63	hemicellulose-derived saccharides	31:63	hemicellulose-derived saccharides	31:63	Separation and purification of hemicellulose-derived saccharides from wood hydrolysate by combined process.
26275826	3	34	theme	%	601:601	arg1	dosage					603:608	1.2% dosage	598:608	1.2% dosage with negligible HDSs loss	598:634	The macro-lignin impurities that accounted for 32.2% of NSCs were removed by lime treatment at 1.2% dosage with negligible HDSs loss.
26275826	2	35	theme	resin	424:428	arg1	adsorption					430:439	resin adsorption	424:439	resin adsorption	424:439	In this study, a combined process consisting of lime treatment, resin adsorption, and gel filtration was developed to separate HDSs from NSCs.
26275826	0	36	theme	wood	70:73	arg1	hydrolysate					75:85	wood hydrolysate	70:85	wood hydrolysate	70:85	Separation and purification of hemicellulose-derived saccharides from wood hydrolysate by combined process.
26275826	1	37	theme	carbohydrate	334:345	arg1	degradation					347:357	carbohydrate degradation	334:357	carbohydrate degradation	334:357	Prehydrolysis of wood biomass prior to kraft cooking provides a stream containing hemicellulose-derived saccharides (HDSs) but also undesired non-saccharide compounds (NSCs) that were resulted from lignin depolymerization and carbohydrate degradation.
26275826	1	38	contain	containing	179:188	arg2	compounds					265:273	undesired non-saccharide compounds	240:273	hemicellulose-derived saccharides (HDSs) but also undesired non-saccharide compounds (NSCs)	190:280	Prehydrolysis of wood biomass prior to kraft cooking provides a stream containing hemicellulose-derived saccharides (HDSs) but also undesired non-saccharide compounds (NSCs) that were resulted from lignin depolymerization and carbohydrate degradation.
26275826	1	38	contain	containing	179:188	arg2	saccharides					212:222	hemicellulose-derived saccharides	190:222	hemicellulose-derived saccharides (HDSs) but also undesired non-saccharide compounds (NSCs)	190:280	Prehydrolysis of wood biomass prior to kraft cooking provides a stream containing hemicellulose-derived saccharides (HDSs) but also undesired non-saccharide compounds (NSCs) that were resulted from lignin depolymerization and carbohydrate degradation.
26275826	1	38	contain	containing	179:188	arg2	NSCs					276:279	NSCs	276:279	NSCs	276:279	Prehydrolysis of wood biomass prior to kraft cooking provides a stream containing hemicellulose-derived saccharides (HDSs) but also undesired non-saccharide compounds (NSCs) that were resulted from lignin depolymerization and carbohydrate degradation.
26275826	1	38	contain	containing	179:188	arg2	HDSs					225:228	HDSs	225:228	HDSs	225:228	Prehydrolysis of wood biomass prior to kraft cooking provides a stream containing hemicellulose-derived saccharides (HDSs) but also undesired non-saccharide compounds (NSCs) that were resulted from lignin depolymerization and carbohydrate degradation.
26275826	1	38	contain	containing	179:188	arg1	stream					172:177	a stream	170:177	a stream containing hemicellulose-derived saccharides (HDSs) but also undesired non-saccharide compounds (NSCs) that were resulted from lignin depolymerization and carbohydrate degradation	170:357	Prehydrolysis of wood biomass prior to kraft cooking provides a stream containing hemicellulose-derived saccharides (HDSs) but also undesired non-saccharide compounds (NSCs) that were resulted from lignin depolymerization and carbohydrate degradation.
26275826	1	39	theme	biomass	130:136	arg1	Prehydrolysis					108:120	Prehydrolysis	108:120	Prehydrolysis of wood biomass prior to kraft cooking	108:159	Prehydrolysis of wood biomass prior to kraft cooking provides a stream containing hemicellulose-derived saccharides (HDSs) but also undesired non-saccharide compounds (NSCs) that were resulted from lignin depolymerization and carbohydrate degradation.
26275826	5	40	theme	remaining	771:779	arg1	hydroxymethylfurfural					800:820	hydroxymethylfurfural	800:820	hydroxymethylfurfural	800:820	The remaining NSCs, furfural and hydroxymethylfurfural, were excluded from HDSs by gel filtration.
26275826	5	40	theme	remaining	771:779	arg1	furfural					787:794	furfural	787:794	furfural	787:794	The remaining NSCs, furfural and hydroxymethylfurfural, were excluded from HDSs by gel filtration.
26275826	5	40	theme	remaining	771:779	arg1	NSCs					781:784	The remaining NSCs	767:784	The remaining NSCs	767:784	The remaining NSCs, furfural and hydroxymethylfurfural, were excluded from HDSs by gel filtration.
26275826	3	41	theme	macro-lignin	507:518	arg1	impurities					520:529	The macro-lignin impurities	503:529	The macro-lignin impurities that accounted for 32.2% of NSCs	503:562	The macro-lignin impurities that accounted for 32.2% of NSCs were removed by lime treatment at 1.2% dosage with negligible HDSs loss.
26275826	3	42	theme	negligible	615:624	arg1	loss					631:634	negligible HDSs loss	615:634	negligible HDSs loss	615:634	The macro-lignin impurities that accounted for 32.2% of NSCs were removed by lime treatment at 1.2% dosage with negligible HDSs loss.
26275826	4	43	theme	ion	714:716	arg1	resin					727:731	mixed bed ion exchange resin	704:731	mixed bed ion exchange resin	704:731	The majority of NSCs, lignin-derived phenolics, were eliminated by mixed bed ion exchange resin, elevating NSCs removal to 94.0%.
26275826	1	44	theme	undesired	240:248	arg1	compounds					265:273	undesired non-saccharide compounds	240:273	hemicellulose-derived saccharides (HDSs) but also undesired non-saccharide compounds (NSCs)	190:280	Prehydrolysis of wood biomass prior to kraft cooking provides a stream containing hemicellulose-derived saccharides (HDSs) but also undesired non-saccharide compounds (NSCs) that were resulted from lignin depolymerization and carbohydrate degradation.
26275826	1	44	theme	undesired	240:248	arg1	NSCs					276:279	NSCs	276:279	NSCs	276:279	Prehydrolysis of wood biomass prior to kraft cooking provides a stream containing hemicellulose-derived saccharides (HDSs) but also undesired non-saccharide compounds (NSCs) that were resulted from lignin depolymerization and carbohydrate degradation.
26275826	3	45	theme	HDSs	626:629	arg1	loss					631:634	negligible HDSs loss	615:634	negligible HDSs loss	615:634	The macro-lignin impurities that accounted for 32.2% of NSCs were removed by lime treatment at 1.2% dosage with negligible HDSs loss.
26275826	1	46	theme	kraft	147:151	arg1	cooking					153:159	kraft cooking	147:159	kraft cooking	147:159	Prehydrolysis of wood biomass prior to kraft cooking provides a stream containing hemicellulose-derived saccharides (HDSs) but also undesired non-saccharide compounds (NSCs) that were resulted from lignin depolymerization and carbohydrate degradation.
26275826	1	47	theme	non-saccharide	250:263	arg1	compounds					265:273	undesired non-saccharide compounds	240:273	hemicellulose-derived saccharides (HDSs) but also undesired non-saccharide compounds (NSCs)	190:280	Prehydrolysis of wood biomass prior to kraft cooking provides a stream containing hemicellulose-derived saccharides (HDSs) but also undesired non-saccharide compounds (NSCs) that were resulted from lignin depolymerization and carbohydrate degradation.
26275826	1	47	theme	non-saccharide	250:263	arg1	NSCs					276:279	NSCs	276:279	NSCs	276:279	Prehydrolysis of wood biomass prior to kraft cooking provides a stream containing hemicellulose-derived saccharides (HDSs) but also undesired non-saccharide compounds (NSCs) that were resulted from lignin depolymerization and carbohydrate degradation.
26275826	3	48	with	dosage	603:608	arg1	loss					631:634	negligible HDSs loss	615:634	negligible HDSs loss	615:634	The macro-lignin impurities that accounted for 32.2% of NSCs were removed by lime treatment at 1.2% dosage with negligible HDSs loss.
26275826	2	49	theme	separate	478:485	arg1	HDSs					487:490	separate HDSs	478:490	separate HDSs from NSCs	478:500	In this study, a combined process consisting of lime treatment, resin adsorption, and gel filtration was developed to separate HDSs from NSCs.
28238909	4	0	theme	diet	517:520	arg1	rats					532:535	High-fat diet (HFD) fed rats	508:535	High-fat diet (HFD) fed rats	508:535	High-fat diet (HFD) fed rats were treated for 14 weeks with AP.
28238909	8	1	theme	chronic	1001:1007	arg1	inflammation					1009:1020	chronic inflammation	1001:1020	chronic inflammation	1001:1020	Moreover, AP dramatically alleviated dysbiosis-associated gut permeability and chronic inflammation with decreased plasma LBP, up-regulation of Occludin, down-regulation of tumor necrosis factor a (TNF-a), monocyte chemotactic protein 1 (MCP-1), chemokine ligand 1 (CXCL-1) and interleukin 1 beta (IL-1β).
28238909	8	2	theme	tumor	1095:1099	arg1	TNF-a					1120:1124	TNF-a	1120:1124	TNF-a	1120:1124	Moreover, AP dramatically alleviated dysbiosis-associated gut permeability and chronic inflammation with decreased plasma LBP, up-regulation of Occludin, down-regulation of tumor necrosis factor a (TNF-a), monocyte chemotactic protein 1 (MCP-1), chemokine ligand 1 (CXCL-1) and interleukin 1 beta (IL-1β).
28238909	8	2	theme	tumor	1095:1099	arg1	a					1117:1117	tumor necrosis factor a	1095:1117	tumor necrosis factor a (TNF-a)	1095:1125	Moreover, AP dramatically alleviated dysbiosis-associated gut permeability and chronic inflammation with decreased plasma LBP, up-regulation of Occludin, down-regulation of tumor necrosis factor a (TNF-a), monocyte chemotactic protein 1 (MCP-1), chemokine ligand 1 (CXCL-1) and interleukin 1 beta (IL-1β).
28238909	0	3	theme	gut	89:91	arg1	permeability					93:104	gut permeability	89:104	gut permeability in HFD-fed rats	89:120	Apple Polysaccharide inhibits microbial dysbiosis and chronic inflammation and modulates gut permeability in HFD-fed rats.
28238909	3	4	theme	apple	463:467	arg1	AP					486:487	AP	486:487	AP	486:487	Therefore, this study investigates the effects of apple polysaccharides (AP) on gut dysbiosis.
28238909	3	4	theme	apple	463:467	arg1	polysaccharides					469:483	apple polysaccharides	463:483	apple polysaccharides (AP)	463:488	Therefore, this study investigates the effects of apple polysaccharides (AP) on gut dysbiosis.
28238909	8	5	theme	dysbiosis-associated	959:978	arg1	permeability					984:995	dysbiosis-associated gut permeability	959:995	dysbiosis-associated gut permeability	959:995	Moreover, AP dramatically alleviated dysbiosis-associated gut permeability and chronic inflammation with decreased plasma LBP, up-regulation of Occludin, down-regulation of tumor necrosis factor a (TNF-a), monocyte chemotactic protein 1 (MCP-1), chemokine ligand 1 (CXCL-1) and interleukin 1 beta (IL-1β).
28238909	1	6	theme	numerous	212:219	arg1	studies					221:227	numerous studies	212:227	numerous studies	212:227	The saying "An apple a day keeps the doctor away" has been known for over 150 years, and numerous studies have shown that apple consumption is closely associated with reduced risks of chronic diseases.
28238909	0	7	theme	HFD-fed	109:115	arg1	rats					117:120	HFD-fed rats	109:120	HFD-fed rats	109:120	Apple Polysaccharide inhibits microbial dysbiosis and chronic inflammation and modulates gut permeability in HFD-fed rats.
28238909	1	8	theme	chronic	307:313	arg1	diseases					315:322	chronic diseases	307:322	chronic diseases	307:322	The saying "An apple a day keeps the doctor away" has been known for over 150 years, and numerous studies have shown that apple consumption is closely associated with reduced risks of chronic diseases.
28238909	5	9	theme	microbiota	576:585	arg1	composition					587:597	The microbiota composition	572:597	The microbiota composition	572:597	The microbiota composition, microbiota-generated short chain fatty acids (SCFAs), gut permeability and chronic inflammation were analyzed.
28238909	10	10	theme	dysbiosis	1506:1514	arg1	rats					1516:1519	HFD-induced dysbiosis rats	1494:1519	HFD-induced dysbiosis rats	1494:1519	Therefore, AP exerts health benefits through inhibiting gut dysbiosis and chronic inflammation and modulating gut permeability in HFD-induced dysbiosis rats.
28238909	4	11	theme	fed	528:530	arg1	rats					532:535	High-fat diet (HFD) fed rats	508:535	High-fat diet (HFD) fed rats	508:535	High-fat diet (HFD) fed rats were treated for 14 weeks with AP.
28238909	10	12	theme	HFD-induced	1494:1504	arg1	rats					1516:1519	HFD-induced dysbiosis rats	1494:1519	HFD-induced dysbiosis rats	1494:1519	Therefore, AP exerts health benefits through inhibiting gut dysbiosis and chronic inflammation and modulating gut permeability in HFD-induced dysbiosis rats.
28238909	1	13	theme	diseases	315:322	arg1	risks					298:302	reduced risks	290:302	reduced risks of chronic diseases	290:322	The saying "An apple a day keeps the doctor away" has been known for over 150 years, and numerous studies have shown that apple consumption is closely associated with reduced risks of chronic diseases.
28238909	6	14	theme	higher	731:736	arg1	abundance					738:746	higher abundance	731:746	higher abundance of Bacteroidetes and Lactobacillus while lower Firmicutes and Fusobacteium	731:821	AP treatment showed higher abundance of Bacteroidetes and Lactobacillus while lower Firmicutes and Fusobacteium.
28238909	3	15	theme	gut	493:495	arg1	dysbiosis					497:505	gut dysbiosis	493:505	gut dysbiosis	493:505	Therefore, this study investigates the effects of apple polysaccharides (AP) on gut dysbiosis.
28238909	9	16	theme	autophagy	1337:1345	arg1	induction					1324:1332	the induction	1320:1332	the induction of autophagy in goblet cells	1320:1361	The potential mechanism is due to the fact that AP reduces gut permeability, which involves the induction of autophagy in goblet cells.
28238909	5	17	theme	gut	654:656	arg1	permeability					658:669	gut permeability	654:669	gut permeability	654:669	The microbiota composition, microbiota-generated short chain fatty acids (SCFAs), gut permeability and chronic inflammation were analyzed.
28238909	9	18	theme	potential	1232:1240	arg1	due					1255:1257	due	1255:1257	due	1255:1257	The potential mechanism is due to the fact that AP reduces gut permeability, which involves the induction of autophagy in goblet cells.
28238909	9	18	theme	potential	1232:1240	arg1	mechanism					1242:1250	The potential mechanism	1228:1250	The potential mechanism	1228:1250	The potential mechanism is due to the fact that AP reduces gut permeability, which involves the induction of autophagy in goblet cells.
28238909	8	19	theme	plasma	1037:1042	arg1	LBP					1044:1046	decreased plasma LBP	1027:1046	decreased plasma LBP	1027:1046	Moreover, AP dramatically alleviated dysbiosis-associated gut permeability and chronic inflammation with decreased plasma LBP, up-regulation of Occludin, down-regulation of tumor necrosis factor a (TNF-a), monocyte chemotactic protein 1 (MCP-1), chemokine ligand 1 (CXCL-1) and interleukin 1 beta (IL-1β).
28238909	7	20	theme	isobutyric	905:914	arg1	acid					916:919	isobutyric acid	905:919	isobutyric acid	905:919	AP significantly increased total SCFAs level that contributed by acetic acid and isobutyric acid.
28238909	8	21	theme	chemokine	1168:1176	arg1	CXCL-1					1188:1193	CXCL-1	1188:1193	CXCL-1	1188:1193	Moreover, AP dramatically alleviated dysbiosis-associated gut permeability and chronic inflammation with decreased plasma LBP, up-regulation of Occludin, down-regulation of tumor necrosis factor a (TNF-a), monocyte chemotactic protein 1 (MCP-1), chemokine ligand 1 (CXCL-1) and interleukin 1 beta (IL-1β).
28238909	8	21	theme	chemokine	1168:1176	arg1	ligand					1178:1183	chemokine ligand 1	1168:1185	chemokine ligand 1 (CXCL-1)	1168:1194	Moreover, AP dramatically alleviated dysbiosis-associated gut permeability and chronic inflammation with decreased plasma LBP, up-regulation of Occludin, down-regulation of tumor necrosis factor a (TNF-a), monocyte chemotactic protein 1 (MCP-1), chemokine ligand 1 (CXCL-1) and interleukin 1 beta (IL-1β).
28238909	8	22	theme	decreased	1027:1035	arg1	LBP					1044:1046	decreased plasma LBP	1027:1046	decreased plasma LBP	1027:1046	Moreover, AP dramatically alleviated dysbiosis-associated gut permeability and chronic inflammation with decreased plasma LBP, up-regulation of Occludin, down-regulation of tumor necrosis factor a (TNF-a), monocyte chemotactic protein 1 (MCP-1), chemokine ligand 1 (CXCL-1) and interleukin 1 beta (IL-1β).
28238909	7	23	theme	acetic	889:894	arg1	acid					896:899	acetic acid	889:899	acetic acid	889:899	AP significantly increased total SCFAs level that contributed by acetic acid and isobutyric acid.
28238909	2	24	theme	chronic	395:401	arg1	diseases					403:410	various chronic diseases	387:410	various chronic diseases	387:410	It has been well accepted that dysbiosis is the reflection of various chronic diseases.
28238909	10	25	theme	gut	1474:1476	arg1	permeability					1478:1489	gut permeability	1474:1489	gut permeability in HFD-induced dysbiosis rats	1474:1519	Therefore, AP exerts health benefits through inhibiting gut dysbiosis and chronic inflammation and modulating gut permeability in HFD-induced dysbiosis rats.
28238909	8	26	theme	chemotactic	1137:1147	arg1	protein					1149:1155	monocyte chemotactic protein 1	1128:1157	monocyte chemotactic protein 1 (MCP-1)	1128:1165	Moreover, AP dramatically alleviated dysbiosis-associated gut permeability and chronic inflammation with decreased plasma LBP, up-regulation of Occludin, down-regulation of tumor necrosis factor a (TNF-a), monocyte chemotactic protein 1 (MCP-1), chemokine ligand 1 (CXCL-1) and interleukin 1 beta (IL-1β).
28238909	8	26	theme	chemotactic	1137:1147	arg1	MCP-1					1160:1164	MCP-1	1160:1164	MCP-1	1160:1164	Moreover, AP dramatically alleviated dysbiosis-associated gut permeability and chronic inflammation with decreased plasma LBP, up-regulation of Occludin, down-regulation of tumor necrosis factor a (TNF-a), monocyte chemotactic protein 1 (MCP-1), chemokine ligand 1 (CXCL-1) and interleukin 1 beta (IL-1β).
28238909	2	27	theme	diseases	403:410	arg1	dysbiosis					356:364	dysbiosis	356:364	dysbiosis	356:364	It has been well accepted that dysbiosis is the reflection of various chronic diseases.
28238909	2	27	theme	diseases	403:410	arg1	reflection					373:382	the reflection	369:382	the reflection of various chronic diseases	369:410	It has been well accepted that dysbiosis is the reflection of various chronic diseases.
28238909	6	28	theme	AP	711:712	arg1	treatment					714:722	AP treatment	711:722	AP treatment	711:722	AP treatment showed higher abundance of Bacteroidetes and Lactobacillus while lower Firmicutes and Fusobacteium.
28238909	0	29	theme	Apple	0:4	arg1	Polysaccharide					6:19	Apple Polysaccharide	0:19	Apple Polysaccharide	0:19	Apple Polysaccharide inhibits microbial dysbiosis and chronic inflammation and modulates gut permeability in HFD-fed rats.
28238909	2	30	theme	various	387:393	arg1	diseases					403:410	various chronic diseases	387:410	various chronic diseases	387:410	It has been well accepted that dysbiosis is the reflection of various chronic diseases.
28238909	10	31	theme	health	1385:1390	arg1	benefits					1392:1399	health benefits	1385:1399	health benefits	1385:1399	Therefore, AP exerts health benefits through inhibiting gut dysbiosis and chronic inflammation and modulating gut permeability in HFD-induced dysbiosis rats.
28238909	5	32	theme	fatty	633:637	arg1	acids					639:643	microbiota-generated short chain fatty acids	600:643	microbiota-generated short chain fatty acids (SCFAs)	600:651	The microbiota composition, microbiota-generated short chain fatty acids (SCFAs), gut permeability and chronic inflammation were analyzed.
28238909	5	32	theme	fatty	633:637	arg1	SCFAs					646:650	SCFAs	646:650	SCFAs	646:650	The microbiota composition, microbiota-generated short chain fatty acids (SCFAs), gut permeability and chronic inflammation were analyzed.
28238909	3	33	from	effects	452:458	arg1	dysbiosis					497:505	gut dysbiosis	493:505	gut dysbiosis	493:505	Therefore, this study investigates the effects of apple polysaccharides (AP) on gut dysbiosis.
28238909	1	34	dep	apple	138:142	arg1	keeps					150:154	keeps	150:154	keeps the doctor away	150:170	The saying "An apple a day keeps the doctor away" has been known for over 150 years, and numerous studies have shown that apple consumption is closely associated with reduced risks of chronic diseases.
28238909	9	35	theme	gut	1287:1289	arg1	permeability					1291:1302	gut permeability	1287:1302	gut permeability	1287:1302	The potential mechanism is due to the fact that AP reduces gut permeability, which involves the induction of autophagy in goblet cells.
28238909	8	36	theme	interleukin	1200:1210	arg1	IL-1β					1220:1224	IL-1β	1220:1224	IL-1β	1220:1224	Moreover, AP dramatically alleviated dysbiosis-associated gut permeability and chronic inflammation with decreased plasma LBP, up-regulation of Occludin, down-regulation of tumor necrosis factor a (TNF-a), monocyte chemotactic protein 1 (MCP-1), chemokine ligand 1 (CXCL-1) and interleukin 1 beta (IL-1β).
28238909	8	36	theme	interleukin	1200:1210	arg1	beta					1214:1217	interleukin 1 beta	1200:1217	interleukin 1 beta (IL-1β)	1200:1225	Moreover, AP dramatically alleviated dysbiosis-associated gut permeability and chronic inflammation with decreased plasma LBP, up-regulation of Occludin, down-regulation of tumor necrosis factor a (TNF-a), monocyte chemotactic protein 1 (MCP-1), chemokine ligand 1 (CXCL-1) and interleukin 1 beta (IL-1β).
28238909	1	37	theme	apple	245:249	arg1	consumption					251:261	apple consumption	245:261	apple consumption	245:261	The saying "An apple a day keeps the doctor away" has been known for over 150 years, and numerous studies have shown that apple consumption is closely associated with reduced risks of chronic diseases.
28238909	0	38	from	permeability	93:104	arg1	rats					117:120	HFD-fed rats	109:120	HFD-fed rats	109:120	Apple Polysaccharide inhibits microbial dysbiosis and chronic inflammation and modulates gut permeability in HFD-fed rats.
28238909	1	39	theme	apple	138:142	arg1	"					171:171	The saying "An apple a day keeps the doctor away"	123:171	The saying "An apple a day keeps the doctor away"	123:171	The saying "An apple a day keeps the doctor away" has been known for over 150 years, and numerous studies have shown that apple consumption is closely associated with reduced risks of chronic diseases.
28238909	4	40	theme	High-fat	508:515	arg1	HFD					523:525	HFD	523:525	HFD	523:525	High-fat diet (HFD) fed rats were treated for 14 weeks with AP.
28238909	4	40	theme	High-fat	508:515	arg1	diet					517:520	High-fat diet	508:520	High-fat diet (HFD) fed rats	508:535	High-fat diet (HFD) fed rats were treated for 14 weeks with AP.
28238909	8	41	theme	Occludin	1066:1073	arg1	MCP-1					1160:1164	MCP-1	1160:1164	MCP-1	1160:1164	Moreover, AP dramatically alleviated dysbiosis-associated gut permeability and chronic inflammation with decreased plasma LBP, up-regulation of Occludin, down-regulation of tumor necrosis factor a (TNF-a), monocyte chemotactic protein 1 (MCP-1), chemokine ligand 1 (CXCL-1) and interleukin 1 beta (IL-1β).
28238909	8	41	theme	Occludin	1066:1073	arg1	CXCL-1					1188:1193	CXCL-1	1188:1193	CXCL-1	1188:1193	Moreover, AP dramatically alleviated dysbiosis-associated gut permeability and chronic inflammation with decreased plasma LBP, up-regulation of Occludin, down-regulation of tumor necrosis factor a (TNF-a), monocyte chemotactic protein 1 (MCP-1), chemokine ligand 1 (CXCL-1) and interleukin 1 beta (IL-1β).
28238909	8	41	theme	Occludin	1066:1073	arg1	up-regulation					1049:1061	up-regulation	1049:1061	up-regulation of Occludin	1049:1073	Moreover, AP dramatically alleviated dysbiosis-associated gut permeability and chronic inflammation with decreased plasma LBP, up-regulation of Occludin, down-regulation of tumor necrosis factor a (TNF-a), monocyte chemotactic protein 1 (MCP-1), chemokine ligand 1 (CXCL-1) and interleukin 1 beta (IL-1β).
28238909	8	41	theme	Occludin	1066:1073	arg1	IL-1β					1220:1224	IL-1β	1220:1224	IL-1β	1220:1224	Moreover, AP dramatically alleviated dysbiosis-associated gut permeability and chronic inflammation with decreased plasma LBP, up-regulation of Occludin, down-regulation of tumor necrosis factor a (TNF-a), monocyte chemotactic protein 1 (MCP-1), chemokine ligand 1 (CXCL-1) and interleukin 1 beta (IL-1β).
28238909	8	41	theme	Occludin	1066:1073	arg1	ligand					1178:1183	chemokine ligand 1	1168:1185	chemokine ligand 1 (CXCL-1)	1168:1194	Moreover, AP dramatically alleviated dysbiosis-associated gut permeability and chronic inflammation with decreased plasma LBP, up-regulation of Occludin, down-regulation of tumor necrosis factor a (TNF-a), monocyte chemotactic protein 1 (MCP-1), chemokine ligand 1 (CXCL-1) and interleukin 1 beta (IL-1β).
28238909	8	41	theme	Occludin	1066:1073	arg1	protein					1149:1155	monocyte chemotactic protein 1	1128:1157	monocyte chemotactic protein 1 (MCP-1)	1128:1165	Moreover, AP dramatically alleviated dysbiosis-associated gut permeability and chronic inflammation with decreased plasma LBP, up-regulation of Occludin, down-regulation of tumor necrosis factor a (TNF-a), monocyte chemotactic protein 1 (MCP-1), chemokine ligand 1 (CXCL-1) and interleukin 1 beta (IL-1β).
28238909	8	41	theme	Occludin	1066:1073	arg1	LBP					1044:1046	decreased plasma LBP	1027:1046	decreased plasma LBP	1027:1046	Moreover, AP dramatically alleviated dysbiosis-associated gut permeability and chronic inflammation with decreased plasma LBP, up-regulation of Occludin, down-regulation of tumor necrosis factor a (TNF-a), monocyte chemotactic protein 1 (MCP-1), chemokine ligand 1 (CXCL-1) and interleukin 1 beta (IL-1β).
28238909	8	41	theme	Occludin	1066:1073	arg1	down-regulation					1076:1090	down-regulation	1076:1090	down-regulation of tumor necrosis factor a (TNF-a)	1076:1125	Moreover, AP dramatically alleviated dysbiosis-associated gut permeability and chronic inflammation with decreased plasma LBP, up-regulation of Occludin, down-regulation of tumor necrosis factor a (TNF-a), monocyte chemotactic protein 1 (MCP-1), chemokine ligand 1 (CXCL-1) and interleukin 1 beta (IL-1β).
28238909	8	41	theme	Occludin	1066:1073	arg1	beta					1214:1217	interleukin 1 beta	1200:1217	interleukin 1 beta (IL-1β)	1200:1225	Moreover, AP dramatically alleviated dysbiosis-associated gut permeability and chronic inflammation with decreased plasma LBP, up-regulation of Occludin, down-regulation of tumor necrosis factor a (TNF-a), monocyte chemotactic protein 1 (MCP-1), chemokine ligand 1 (CXCL-1) and interleukin 1 beta (IL-1β).
28238909	0	42	theme	microbial	30:38	arg1	dysbiosis					40:48	microbial dysbiosis	30:48	microbial dysbiosis	30:48	Apple Polysaccharide inhibits microbial dysbiosis and chronic inflammation and modulates gut permeability in HFD-fed rats.
28238909	8	43	theme	monocyte	1128:1135	arg1	protein					1149:1155	monocyte chemotactic protein 1	1128:1157	monocyte chemotactic protein 1 (MCP-1)	1128:1165	Moreover, AP dramatically alleviated dysbiosis-associated gut permeability and chronic inflammation with decreased plasma LBP, up-regulation of Occludin, down-regulation of tumor necrosis factor a (TNF-a), monocyte chemotactic protein 1 (MCP-1), chemokine ligand 1 (CXCL-1) and interleukin 1 beta (IL-1β).
28238909	8	43	theme	monocyte	1128:1135	arg1	MCP-1					1160:1164	MCP-1	1160:1164	MCP-1	1160:1164	Moreover, AP dramatically alleviated dysbiosis-associated gut permeability and chronic inflammation with decreased plasma LBP, up-regulation of Occludin, down-regulation of tumor necrosis factor a (TNF-a), monocyte chemotactic protein 1 (MCP-1), chemokine ligand 1 (CXCL-1) and interleukin 1 beta (IL-1β).
28238909	6	44	dep	lower	789:793	arg1	Firmicutes					795:804	Firmicutes	795:804	Firmicutes	795:804	AP treatment showed higher abundance of Bacteroidetes and Lactobacillus while lower Firmicutes and Fusobacteium.
28238909	10	45	theme	chronic	1438:1444	arg1	inflammation					1446:1457	chronic inflammation	1438:1457	chronic inflammation	1438:1457	Therefore, AP exerts health benefits through inhibiting gut dysbiosis and chronic inflammation and modulating gut permeability in HFD-induced dysbiosis rats.
28238909	8	46	theme	factor	1110:1115	arg1	TNF-a					1120:1124	TNF-a	1120:1124	TNF-a	1120:1124	Moreover, AP dramatically alleviated dysbiosis-associated gut permeability and chronic inflammation with decreased plasma LBP, up-regulation of Occludin, down-regulation of tumor necrosis factor a (TNF-a), monocyte chemotactic protein 1 (MCP-1), chemokine ligand 1 (CXCL-1) and interleukin 1 beta (IL-1β).
28238909	8	46	theme	factor	1110:1115	arg1	a					1117:1117	tumor necrosis factor a	1095:1117	tumor necrosis factor a (TNF-a)	1095:1125	Moreover, AP dramatically alleviated dysbiosis-associated gut permeability and chronic inflammation with decreased plasma LBP, up-regulation of Occludin, down-regulation of tumor necrosis factor a (TNF-a), monocyte chemotactic protein 1 (MCP-1), chemokine ligand 1 (CXCL-1) and interleukin 1 beta (IL-1β).
28238909	8	47	theme	necrosis	1101:1108	arg1	TNF-a					1120:1124	TNF-a	1120:1124	TNF-a	1120:1124	Moreover, AP dramatically alleviated dysbiosis-associated gut permeability and chronic inflammation with decreased plasma LBP, up-regulation of Occludin, down-regulation of tumor necrosis factor a (TNF-a), monocyte chemotactic protein 1 (MCP-1), chemokine ligand 1 (CXCL-1) and interleukin 1 beta (IL-1β).
28238909	8	47	theme	necrosis	1101:1108	arg1	a					1117:1117	tumor necrosis factor a	1095:1117	tumor necrosis factor a (TNF-a)	1095:1125	Moreover, AP dramatically alleviated dysbiosis-associated gut permeability and chronic inflammation with decreased plasma LBP, up-regulation of Occludin, down-regulation of tumor necrosis factor a (TNF-a), monocyte chemotactic protein 1 (MCP-1), chemokine ligand 1 (CXCL-1) and interleukin 1 beta (IL-1β).
28238909	0	48	theme	chronic	54:60	arg1	inflammation					62:73	chronic inflammation	54:73	chronic inflammation	54:73	Apple Polysaccharide inhibits microbial dysbiosis and chronic inflammation and modulates gut permeability in HFD-fed rats.
28238909	5	49	theme	microbiota-generated	600:619	arg1	acids					639:643	microbiota-generated short chain fatty acids	600:643	microbiota-generated short chain fatty acids (SCFAs)	600:651	The microbiota composition, microbiota-generated short chain fatty acids (SCFAs), gut permeability and chronic inflammation were analyzed.
28238909	5	49	theme	microbiota-generated	600:619	arg1	SCFAs					646:650	SCFAs	646:650	SCFAs	646:650	The microbiota composition, microbiota-generated short chain fatty acids (SCFAs), gut permeability and chronic inflammation were analyzed.
28238909	9	50	from	induction	1324:1332	arg1	cells					1357:1361	goblet cells	1350:1361	goblet cells	1350:1361	The potential mechanism is due to the fact that AP reduces gut permeability, which involves the induction of autophagy in goblet cells.
28238909	10	51	from	permeability	1478:1489	arg1	rats					1516:1519	HFD-induced dysbiosis rats	1494:1519	HFD-induced dysbiosis rats	1494:1519	Therefore, AP exerts health benefits through inhibiting gut dysbiosis and chronic inflammation and modulating gut permeability in HFD-induced dysbiosis rats.
28238909	1	52	theme	saying	127:132	arg1	"					171:171	The saying "An apple a day keeps the doctor away"	123:171	The saying "An apple a day keeps the doctor away"	123:171	The saying "An apple a day keeps the doctor away" has been known for over 150 years, and numerous studies have shown that apple consumption is closely associated with reduced risks of chronic diseases.
28238909	8	53	theme	gut	980:982	arg1	permeability					984:995	dysbiosis-associated gut permeability	959:995	dysbiosis-associated gut permeability	959:995	Moreover, AP dramatically alleviated dysbiosis-associated gut permeability and chronic inflammation with decreased plasma LBP, up-regulation of Occludin, down-regulation of tumor necrosis factor a (TNF-a), monocyte chemotactic protein 1 (MCP-1), chemokine ligand 1 (CXCL-1) and interleukin 1 beta (IL-1β).
28238909	5	54	theme	short	621:625	arg1	acids					639:643	microbiota-generated short chain fatty acids	600:643	microbiota-generated short chain fatty acids (SCFAs)	600:651	The microbiota composition, microbiota-generated short chain fatty acids (SCFAs), gut permeability and chronic inflammation were analyzed.
28238909	5	54	theme	short	621:625	arg1	SCFAs					646:650	SCFAs	646:650	SCFAs	646:650	The microbiota composition, microbiota-generated short chain fatty acids (SCFAs), gut permeability and chronic inflammation were analyzed.
28238909	6	55	theme	Lactobacillus	769:781	arg1	abundance					738:746	higher abundance	731:746	higher abundance of Bacteroidetes and Lactobacillus while lower Firmicutes and Fusobacteium	731:821	AP treatment showed higher abundance of Bacteroidetes and Lactobacillus while lower Firmicutes and Fusobacteium.
28238909	9	56	theme	goblet	1350:1355	arg1	cells					1357:1361	goblet cells	1350:1361	goblet cells	1350:1361	The potential mechanism is due to the fact that AP reduces gut permeability, which involves the induction of autophagy in goblet cells.
28238909	5	57	theme	chain	627:631	arg1	acids					639:643	microbiota-generated short chain fatty acids	600:643	microbiota-generated short chain fatty acids (SCFAs)	600:651	The microbiota composition, microbiota-generated short chain fatty acids (SCFAs), gut permeability and chronic inflammation were analyzed.
28238909	5	57	theme	chain	627:631	arg1	SCFAs					646:650	SCFAs	646:650	SCFAs	646:650	The microbiota composition, microbiota-generated short chain fatty acids (SCFAs), gut permeability and chronic inflammation were analyzed.
28238909	4	58	with	weeks	557:561	arg1	AP					568:569	AP	568:569	AP	568:569	High-fat diet (HFD) fed rats were treated for 14 weeks with AP.
28238909	7	59	theme	total	851:855	arg1	level					863:867	total SCFAs level	851:867	total SCFAs level that contributed by acetic acid and isobutyric acid	851:919	AP significantly increased total SCFAs level that contributed by acetic acid and isobutyric acid.
28238909	8	60	theme	a	1117:1117	arg1	MCP-1					1160:1164	MCP-1	1160:1164	MCP-1	1160:1164	Moreover, AP dramatically alleviated dysbiosis-associated gut permeability and chronic inflammation with decreased plasma LBP, up-regulation of Occludin, down-regulation of tumor necrosis factor a (TNF-a), monocyte chemotactic protein 1 (MCP-1), chemokine ligand 1 (CXCL-1) and interleukin 1 beta (IL-1β).
28238909	8	60	theme	a	1117:1117	arg1	CXCL-1					1188:1193	CXCL-1	1188:1193	CXCL-1	1188:1193	Moreover, AP dramatically alleviated dysbiosis-associated gut permeability and chronic inflammation with decreased plasma LBP, up-regulation of Occludin, down-regulation of tumor necrosis factor a (TNF-a), monocyte chemotactic protein 1 (MCP-1), chemokine ligand 1 (CXCL-1) and interleukin 1 beta (IL-1β).
28238909	8	60	theme	a	1117:1117	arg1	up-regulation					1049:1061	up-regulation	1049:1061	up-regulation of Occludin	1049:1073	Moreover, AP dramatically alleviated dysbiosis-associated gut permeability and chronic inflammation with decreased plasma LBP, up-regulation of Occludin, down-regulation of tumor necrosis factor a (TNF-a), monocyte chemotactic protein 1 (MCP-1), chemokine ligand 1 (CXCL-1) and interleukin 1 beta (IL-1β).
28238909	8	60	theme	a	1117:1117	arg1	IL-1β					1220:1224	IL-1β	1220:1224	IL-1β	1220:1224	Moreover, AP dramatically alleviated dysbiosis-associated gut permeability and chronic inflammation with decreased plasma LBP, up-regulation of Occludin, down-regulation of tumor necrosis factor a (TNF-a), monocyte chemotactic protein 1 (MCP-1), chemokine ligand 1 (CXCL-1) and interleukin 1 beta (IL-1β).
28238909	8	60	theme	a	1117:1117	arg1	ligand					1178:1183	chemokine ligand 1	1168:1185	chemokine ligand 1 (CXCL-1)	1168:1194	Moreover, AP dramatically alleviated dysbiosis-associated gut permeability and chronic inflammation with decreased plasma LBP, up-regulation of Occludin, down-regulation of tumor necrosis factor a (TNF-a), monocyte chemotactic protein 1 (MCP-1), chemokine ligand 1 (CXCL-1) and interleukin 1 beta (IL-1β).
28238909	8	60	theme	a	1117:1117	arg1	protein					1149:1155	monocyte chemotactic protein 1	1128:1157	monocyte chemotactic protein 1 (MCP-1)	1128:1165	Moreover, AP dramatically alleviated dysbiosis-associated gut permeability and chronic inflammation with decreased plasma LBP, up-regulation of Occludin, down-regulation of tumor necrosis factor a (TNF-a), monocyte chemotactic protein 1 (MCP-1), chemokine ligand 1 (CXCL-1) and interleukin 1 beta (IL-1β).
28238909	8	60	theme	a	1117:1117	arg1	LBP					1044:1046	decreased plasma LBP	1027:1046	decreased plasma LBP	1027:1046	Moreover, AP dramatically alleviated dysbiosis-associated gut permeability and chronic inflammation with decreased plasma LBP, up-regulation of Occludin, down-regulation of tumor necrosis factor a (TNF-a), monocyte chemotactic protein 1 (MCP-1), chemokine ligand 1 (CXCL-1) and interleukin 1 beta (IL-1β).
28238909	8	60	theme	a	1117:1117	arg1	down-regulation					1076:1090	down-regulation	1076:1090	down-regulation of tumor necrosis factor a (TNF-a)	1076:1125	Moreover, AP dramatically alleviated dysbiosis-associated gut permeability and chronic inflammation with decreased plasma LBP, up-regulation of Occludin, down-regulation of tumor necrosis factor a (TNF-a), monocyte chemotactic protein 1 (MCP-1), chemokine ligand 1 (CXCL-1) and interleukin 1 beta (IL-1β).
28238909	8	60	theme	a	1117:1117	arg1	beta					1214:1217	interleukin 1 beta	1200:1217	interleukin 1 beta (IL-1β)	1200:1225	Moreover, AP dramatically alleviated dysbiosis-associated gut permeability and chronic inflammation with decreased plasma LBP, up-regulation of Occludin, down-regulation of tumor necrosis factor a (TNF-a), monocyte chemotactic protein 1 (MCP-1), chemokine ligand 1 (CXCL-1) and interleukin 1 beta (IL-1β).
28238909	6	61	theme	Bacteroidetes	751:763	arg1	abundance					738:746	higher abundance	731:746	higher abundance of Bacteroidetes and Lactobacillus while lower Firmicutes and Fusobacteium	731:821	AP treatment showed higher abundance of Bacteroidetes and Lactobacillus while lower Firmicutes and Fusobacteium.
28238909	3	62	theme	polysaccharides	469:483	arg1	effects					452:458	the effects	448:458	the effects of apple polysaccharides (AP) on gut dysbiosis	448:505	Therefore, this study investigates the effects of apple polysaccharides (AP) on gut dysbiosis.
28238909	7	63	theme	SCFAs	857:861	arg1	level					863:867	total SCFAs level	851:867	total SCFAs level that contributed by acetic acid and isobutyric acid	851:919	AP significantly increased total SCFAs level that contributed by acetic acid and isobutyric acid.
28238909	5	64	theme	chronic	675:681	arg1	inflammation					683:694	chronic inflammation	675:694	chronic inflammation	675:694	The microbiota composition, microbiota-generated short chain fatty acids (SCFAs), gut permeability and chronic inflammation were analyzed.
28238909	1	65	theme	reduced	290:296	arg1	risks					298:302	reduced risks	290:302	reduced risks of chronic diseases	290:322	The saying "An apple a day keeps the doctor away" has been known for over 150 years, and numerous studies have shown that apple consumption is closely associated with reduced risks of chronic diseases.
28238909	10	66	theme	gut	1420:1422	arg1	dysbiosis					1424:1432	gut dysbiosis	1420:1432	gut dysbiosis	1420:1432	Therefore, AP exerts health benefits through inhibiting gut dysbiosis and chronic inflammation and modulating gut permeability in HFD-induced dysbiosis rats.
27702505	2	0	theme	free	390:393	arg1	content					407:413	free amino group content	390:413	free amino group content in the glycosylated CPI	390:437	Upon decreasing of free amino group content in the glycosylated CPI to 72%, a new band near the loading end of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and a shift of CPI peak in high performance size exclusion chromatography confirmed that the covalent attachment of CPI to GA was successful.
27702505	2	1	theme	exclusion	589:597	arg1	chromatography					599:612	high performance size exclusion chromatography	567:612	high performance size exclusion chromatography	567:612	Upon decreasing of free amino group content in the glycosylated CPI to 72%, a new band near the loading end of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and a shift of CPI peak in high performance size exclusion chromatography confirmed that the covalent attachment of CPI to GA was successful.
27702505	2	2	from	peak	559:562	arg1	chromatography					599:612	high performance size exclusion chromatography	567:612	high performance size exclusion chromatography	567:612	Upon decreasing of free amino group content in the glycosylated CPI to 72%, a new band near the loading end of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and a shift of CPI peak in high performance size exclusion chromatography confirmed that the covalent attachment of CPI to GA was successful.
27702505	3	3	theme	structure	707:715	arg1	analysis					717:724	secondary structure analysis	697:724	secondary structure analysis	697:724	The results of secondary structure analysis suggested that grafted CPI had decreased α-helix and β-sheet levels and increased random coils level.
27702505	5	4	theme	90°C	1005:1008	arg1	%					996:996	1%	995:996	1% of GA, 90°C and reaction time 15min	995:1032	The optimal conjugation conditions chosen from the further experiments were 1% of GA, 90°C and reaction time 15min.
27702505	5	4	theme	90°C	1005:1008	arg1	15min					1028:1032	reaction time 15min	1014:1032	reaction time 15min	1014:1032	The optimal conjugation conditions chosen from the further experiments were 1% of GA, 90°C and reaction time 15min.
27702505	5	4	theme	90°C	1005:1008	arg1	conditions					943:952	The optimal conjugation conditions	919:952	The optimal conjugation conditions chosen from the further experiments	919:988	The optimal conjugation conditions chosen from the further experiments were 1% of GA, 90°C and reaction time 15min.
27702505	5	4	theme	90°C	1005:1008	arg1	90°C					1005:1008	90°C	1005:1008	90°C	1005:1008	The optimal conjugation conditions chosen from the further experiments were 1% of GA, 90°C and reaction time 15min.
27702505	5	4	theme	90°C	1005:1008	arg1	GA					1001:1002	GA	1001:1002	GA	1001:1002	The optimal conjugation conditions chosen from the further experiments were 1% of GA, 90°C and reaction time 15min.
27702505	2	5	theme	loading	467:473	arg1	end					475:477	the loading end	463:477	the loading end of sodium dodecyl sulfate-polyacrylamide gel electrophoresis	463:538	Upon decreasing of free amino group content in the glycosylated CPI to 72%, a new band near the loading end of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and a shift of CPI peak in high performance size exclusion chromatography confirmed that the covalent attachment of CPI to GA was successful.
27702505	1	6	theme	study	150:154	arg1	aim					138:140	The aim	134:140	The aim of this study	134:154	The aim of this study was to produce covalently attached conjugate between canola protein isolate (CPI) and gum Arabic (GA) in aqueous solutions via the Maillard reaction at 90°C in a model system consisting of 2% CPI and 1, 2 or 4% GA.
27702505	1	7	dep	GA	367:368	arg1	%					365:365	%	365:365	%	365:365	The aim of this study was to produce covalently attached conjugate between canola protein isolate (CPI) and gum Arabic (GA) in aqueous solutions via the Maillard reaction at 90°C in a model system consisting of 2% CPI and 1, 2 or 4% GA.
27702505	3	8	theme	analysis	717:724	arg1	results					686:692	The results	682:692	The results of secondary structure analysis	682:724	The results of secondary structure analysis suggested that grafted CPI had decreased α-helix and β-sheet levels and increased random coils level.
27702505	0	9	theme	Maillard	86:93	arg1	reaction					95:102	Maillard reaction	86:102	Maillard reaction	86:102	Preparation of chemically modified canola protein isolate with gum Arabic by means of Maillard reaction under wet-heating conditions.
27702505	1	10	theme	gum	242:244	arg1	GA					254:255	GA	254:255	GA	254:255	The aim of this study was to produce covalently attached conjugate between canola protein isolate (CPI) and gum Arabic (GA) in aqueous solutions via the Maillard reaction at 90°C in a model system consisting of 2% CPI and 1, 2 or 4% GA.
27702505	1	10	theme	gum	242:244	arg1	Arabic					246:251	gum Arabic	242:251	gum Arabic (GA)	242:256	The aim of this study was to produce covalently attached conjugate between canola protein isolate (CPI) and gum Arabic (GA) in aqueous solutions via the Maillard reaction at 90°C in a model system consisting of 2% CPI and 1, 2 or 4% GA.
27702505	2	11	theme	electrophoresis	524:538	arg1	end					475:477	the loading end	463:477	the loading end of sodium dodecyl sulfate-polyacrylamide gel electrophoresis	463:538	Upon decreasing of free amino group content in the glycosylated CPI to 72%, a new band near the loading end of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and a shift of CPI peak in high performance size exclusion chromatography confirmed that the covalent attachment of CPI to GA was successful.
27702505	2	12	gly	glycosylated	422:433	arg1	CPI					435:437	the glycosylated CPI	418:437	the glycosylated CPI	418:437	Upon decreasing of free amino group content in the glycosylated CPI to 72%, a new band near the loading end of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and a shift of CPI peak in high performance size exclusion chromatography confirmed that the covalent attachment of CPI to GA was successful.
27702505	3	13	theme	secondary	697:705	arg1	analysis					717:724	secondary structure analysis	697:724	secondary structure analysis	697:724	The results of secondary structure analysis suggested that grafted CPI had decreased α-helix and β-sheet levels and increased random coils level.
27702505	4	14	theme	CPI	846:848	arg1	solubility					832:841	The solubility	828:841	The solubility of CPI at isoelectric point	828:869	The solubility of CPI at isoelectric point was improved remarkably after grafting with GA.
27702505	2	15	theme	sulfate-polyacrylamide	497:518	arg1	electrophoresis					524:538	sodium dodecyl sulfate-polyacrylamide gel electrophoresis	482:538	sodium dodecyl sulfate-polyacrylamide gel electrophoresis	482:538	Upon decreasing of free amino group content in the glycosylated CPI to 72%, a new band near the loading end of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and a shift of CPI peak in high performance size exclusion chromatography confirmed that the covalent attachment of CPI to GA was successful.
27702505	2	16	theme	gel	520:522	arg1	electrophoresis					524:538	sodium dodecyl sulfate-polyacrylamide gel electrophoresis	482:538	sodium dodecyl sulfate-polyacrylamide gel electrophoresis	482:538	Upon decreasing of free amino group content in the glycosylated CPI to 72%, a new band near the loading end of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and a shift of CPI peak in high performance size exclusion chromatography confirmed that the covalent attachment of CPI to GA was successful.
27702505	1	17	theme	model	318:322	arg1	system					324:329	a model system	316:329	a model system consisting of 2% CPI and 1, 2 or 4% GA	316:368	The aim of this study was to produce covalently attached conjugate between canola protein isolate (CPI) and gum Arabic (GA) in aqueous solutions via the Maillard reaction at 90°C in a model system consisting of 2% CPI and 1, 2 or 4% GA.
27702505	5	18	theme	GA	1001:1002	arg1	%					996:996	1%	995:996	1% of GA, 90°C and reaction time 15min	995:1032	The optimal conjugation conditions chosen from the further experiments were 1% of GA, 90°C and reaction time 15min.
27702505	5	18	theme	GA	1001:1002	arg1	15min					1028:1032	reaction time 15min	1014:1032	reaction time 15min	1014:1032	The optimal conjugation conditions chosen from the further experiments were 1% of GA, 90°C and reaction time 15min.
27702505	5	18	theme	GA	1001:1002	arg1	conditions					943:952	The optimal conjugation conditions	919:952	The optimal conjugation conditions chosen from the further experiments	919:988	The optimal conjugation conditions chosen from the further experiments were 1% of GA, 90°C and reaction time 15min.
27702505	5	18	theme	GA	1001:1002	arg1	90°C					1005:1008	90°C	1005:1008	90°C	1005:1008	The optimal conjugation conditions chosen from the further experiments were 1% of GA, 90°C and reaction time 15min.
27702505	5	18	theme	GA	1001:1002	arg1	GA					1001:1002	GA	1001:1002	GA	1001:1002	The optimal conjugation conditions chosen from the further experiments were 1% of GA, 90°C and reaction time 15min.
27702505	2	19	theme	peak	559:562	arg1	shift					546:550	a shift	544:550	a shift of CPI peak in high performance size exclusion chromatography	544:612	Upon decreasing of free amino group content in the glycosylated CPI to 72%, a new band near the loading end of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and a shift of CPI peak in high performance size exclusion chromatography confirmed that the covalent attachment of CPI to GA was successful.
27702505	2	19	theme	peak	559:562	arg1	band					453:456	a new band	447:456	a new band near the loading end of sodium dodecyl sulfate-polyacrylamide gel electrophoresis	447:538	Upon decreasing of free amino group content in the glycosylated CPI to 72%, a new band near the loading end of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and a shift of CPI peak in high performance size exclusion chromatography confirmed that the covalent attachment of CPI to GA was successful.
27702505	4	20	from	point	865:869	arg1	solubility					832:841	The solubility	828:841	The solubility of CPI at isoelectric point	828:869	The solubility of CPI at isoelectric point was improved remarkably after grafting with GA.
27702505	2	21	theme	covalent	633:640	arg1	attachment					642:651	the covalent attachment	629:651	the covalent attachment of CPI to GA	629:664	Upon decreasing of free amino group content in the glycosylated CPI to 72%, a new band near the loading end of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and a shift of CPI peak in high performance size exclusion chromatography confirmed that the covalent attachment of CPI to GA was successful.
27702505	2	21	theme	covalent	633:640	arg1	successful					670:679	successful	670:679	successful	670:679	Upon decreasing of free amino group content in the glycosylated CPI to 72%, a new band near the loading end of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and a shift of CPI peak in high performance size exclusion chromatography confirmed that the covalent attachment of CPI to GA was successful.
27702505	5	22	theme	time	1023:1026	arg1	15min					1028:1032	reaction time 15min	1014:1032	reaction time 15min	1014:1032	The optimal conjugation conditions chosen from the further experiments were 1% of GA, 90°C and reaction time 15min.
27702505	2	23	theme	CPI	555:557	arg1	peak					559:562	CPI peak	555:562	CPI peak in high performance size exclusion chromatography	555:612	Upon decreasing of free amino group content in the glycosylated CPI to 72%, a new band near the loading end of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and a shift of CPI peak in high performance size exclusion chromatography confirmed that the covalent attachment of CPI to GA was successful.
27702505	3	24	theme	α-helix	767:773	arg1	levels					787:792	α-helix and β-sheet levels	767:792	α-helix and β-sheet levels	767:792	The results of secondary structure analysis suggested that grafted CPI had decreased α-helix and β-sheet levels and increased random coils level.
27702505	0	25	theme	modified	26:33	arg1	protein					42:48	chemically modified canola protein	15:48	chemically modified canola protein	15:48	Preparation of chemically modified canola protein isolate with gum Arabic by means of Maillard reaction under wet-heating conditions.
27702505	1	26	theme	attached	182:189	arg1	conjugate					191:199	covalently attached conjugate	171:199	covalently attached conjugate between canola protein isolate (CPI) and gum Arabic (GA) in aqueous solutions	171:277	The aim of this study was to produce covalently attached conjugate between canola protein isolate (CPI) and gum Arabic (GA) in aqueous solutions via the Maillard reaction at 90°C in a model system consisting of 2% CPI and 1, 2 or 4% GA.
27702505	0	27	theme	wet-heating	110:120	arg1	conditions					122:131	wet-heating conditions	110:131	wet-heating conditions	110:131	Preparation of chemically modified canola protein isolate with gum Arabic by means of Maillard reaction under wet-heating conditions.
27702505	3	28	theme	coils	815:819	arg1	level					821:825	random coils level	808:825	random coils level	808:825	The results of secondary structure analysis suggested that grafted CPI had decreased α-helix and β-sheet levels and increased random coils level.
27702505	3	29	theme	random	808:813	arg1	level					821:825	random coils level	808:825	random coils level	808:825	The results of secondary structure analysis suggested that grafted CPI had decreased α-helix and β-sheet levels and increased random coils level.
27702505	4	30	theme	isoelectric	853:863	arg1	point					865:869	isoelectric point	853:869	isoelectric point	853:869	The solubility of CPI at isoelectric point was improved remarkably after grafting with GA.
27702505	0	31	theme	protein	42:48	arg1	Preparation					0:10	Preparation	0:10	Preparation of chemically modified canola protein	0:48	Preparation of chemically modified canola protein isolate with gum Arabic by means of Maillard reaction under wet-heating conditions.
27702505	2	32	theme	CPI	656:658	arg1	attachment					642:651	the covalent attachment	629:651	the covalent attachment of CPI to GA	629:664	Upon decreasing of free amino group content in the glycosylated CPI to 72%, a new band near the loading end of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and a shift of CPI peak in high performance size exclusion chromatography confirmed that the covalent attachment of CPI to GA was successful.
27702505	2	32	theme	CPI	656:658	arg1	successful					670:679	successful	670:679	successful	670:679	Upon decreasing of free amino group content in the glycosylated CPI to 72%, a new band near the loading end of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and a shift of CPI peak in high performance size exclusion chromatography confirmed that the covalent attachment of CPI to GA was successful.
27702505	0	33	theme	canola	35:40	arg1	protein					42:48	chemically modified canola protein	15:48	chemically modified canola protein	15:48	Preparation of chemically modified canola protein isolate with gum Arabic by means of Maillard reaction under wet-heating conditions.
27702505	1	34	theme	aqueous	261:267	arg1	solutions					269:277	aqueous solutions	261:277	aqueous solutions	261:277	The aim of this study was to produce covalently attached conjugate between canola protein isolate (CPI) and gum Arabic (GA) in aqueous solutions via the Maillard reaction at 90°C in a model system consisting of 2% CPI and 1, 2 or 4% GA.
27702505	1	35	from	90°C	308:311	arg1	reaction					296:303	the Maillard reaction	283:303	the Maillard reaction at 90°C in a model system consisting of 2% CPI and 1, 2 or 4% GA	283:368	The aim of this study was to produce covalently attached conjugate between canola protein isolate (CPI) and gum Arabic (GA) in aqueous solutions via the Maillard reaction at 90°C in a model system consisting of 2% CPI and 1, 2 or 4% GA.
27702505	1	36	theme	2	345:345	arg1	%					346:346	%	346:346	%	346:346	The aim of this study was to produce covalently attached conjugate between canola protein isolate (CPI) and gum Arabic (GA) in aqueous solutions via the Maillard reaction at 90°C in a model system consisting of 2% CPI and 1, 2 or 4% GA.
27702505	2	37	theme	size	584:587	arg1	chromatography					599:612	high performance size exclusion chromatography	567:612	high performance size exclusion chromatography	567:612	Upon decreasing of free amino group content in the glycosylated CPI to 72%, a new band near the loading end of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and a shift of CPI peak in high performance size exclusion chromatography confirmed that the covalent attachment of CPI to GA was successful.
27702505	1	38	theme	canola	209:214	arg1	CPI					233:235	CPI	233:235	CPI	233:235	The aim of this study was to produce covalently attached conjugate between canola protein isolate (CPI) and gum Arabic (GA) in aqueous solutions via the Maillard reaction at 90°C in a model system consisting of 2% CPI and 1, 2 or 4% GA.
27702505	1	38	theme	canola	209:214	arg1	isolate					224:230	canola protein isolate	209:230	canola protein isolate (CPI)	209:236	The aim of this study was to produce covalently attached conjugate between canola protein isolate (CPI) and gum Arabic (GA) in aqueous solutions via the Maillard reaction at 90°C in a model system consisting of 2% CPI and 1, 2 or 4% GA.
27702505	2	39	theme	glycosylated	422:433	arg1	CPI					435:437	the glycosylated CPI	418:437	the glycosylated CPI	418:437	Upon decreasing of free amino group content in the glycosylated CPI to 72%, a new band near the loading end of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and a shift of CPI peak in high performance size exclusion chromatography confirmed that the covalent attachment of CPI to GA was successful.
27702505	2	40	attach	attachment	642:651	arg1	GA					663:664	GA	663:664	GA	663:664	Upon decreasing of free amino group content in the glycosylated CPI to 72%, a new band near the loading end of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and a shift of CPI peak in high performance size exclusion chromatography confirmed that the covalent attachment of CPI to GA was successful.
27702505	2	40	attach	attachment	642:651	arg2	CPI					656:658	CPI	656:658	CPI	656:658	Upon decreasing of free amino group content in the glycosylated CPI to 72%, a new band near the loading end of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and a shift of CPI peak in high performance size exclusion chromatography confirmed that the covalent attachment of CPI to GA was successful.
27702505	1	41	theme	%	346:346	arg1	CPI					348:350	2% CPI	345:350	2% CPI	345:350	The aim of this study was to produce covalently attached conjugate between canola protein isolate (CPI) and gum Arabic (GA) in aqueous solutions via the Maillard reaction at 90°C in a model system consisting of 2% CPI and 1, 2 or 4% GA.
27702505	2	42	theme	performance	572:582	arg1	chromatography					599:612	high performance size exclusion chromatography	567:612	high performance size exclusion chromatography	567:612	Upon decreasing of free amino group content in the glycosylated CPI to 72%, a new band near the loading end of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and a shift of CPI peak in high performance size exclusion chromatography confirmed that the covalent attachment of CPI to GA was successful.
27702505	1	43	from	reaction	296:303	arg1	system					324:329	a model system	316:329	a model system consisting of 2% CPI and 1, 2 or 4% GA	316:368	The aim of this study was to produce covalently attached conjugate between canola protein isolate (CPI) and gum Arabic (GA) in aqueous solutions via the Maillard reaction at 90°C in a model system consisting of 2% CPI and 1, 2 or 4% GA.
27702505	5	44	theme	15min	1028:1032	arg1	%					996:996	1%	995:996	1% of GA, 90°C and reaction time 15min	995:1032	The optimal conjugation conditions chosen from the further experiments were 1% of GA, 90°C and reaction time 15min.
27702505	5	44	theme	15min	1028:1032	arg1	15min					1028:1032	reaction time 15min	1014:1032	reaction time 15min	1014:1032	The optimal conjugation conditions chosen from the further experiments were 1% of GA, 90°C and reaction time 15min.
27702505	5	44	theme	15min	1028:1032	arg1	conditions					943:952	The optimal conjugation conditions	919:952	The optimal conjugation conditions chosen from the further experiments	919:988	The optimal conjugation conditions chosen from the further experiments were 1% of GA, 90°C and reaction time 15min.
27702505	5	44	theme	15min	1028:1032	arg1	90°C					1005:1008	90°C	1005:1008	90°C	1005:1008	The optimal conjugation conditions chosen from the further experiments were 1% of GA, 90°C and reaction time 15min.
27702505	5	44	theme	15min	1028:1032	arg1	GA					1001:1002	GA	1001:1002	GA	1001:1002	The optimal conjugation conditions chosen from the further experiments were 1% of GA, 90°C and reaction time 15min.
27702505	2	45	theme	new	449:451	arg1	band					453:456	a new band	447:456	a new band near the loading end of sodium dodecyl sulfate-polyacrylamide gel electrophoresis	447:538	Upon decreasing of free amino group content in the glycosylated CPI to 72%, a new band near the loading end of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and a shift of CPI peak in high performance size exclusion chromatography confirmed that the covalent attachment of CPI to GA was successful.
27702505	5	46	theme	optimal	923:929	arg1	conditions					943:952	The optimal conjugation conditions	919:952	The optimal conjugation conditions chosen from the further experiments	919:988	The optimal conjugation conditions chosen from the further experiments were 1% of GA, 90°C and reaction time 15min.
27702505	5	46	theme	optimal	923:929	arg1	%					996:996	1%	995:996	1% of GA, 90°C and reaction time 15min	995:1032	The optimal conjugation conditions chosen from the further experiments were 1% of GA, 90°C and reaction time 15min.
27702505	5	46	theme	optimal	923:929	arg1	15min					1028:1032	reaction time 15min	1014:1032	reaction time 15min	1014:1032	The optimal conjugation conditions chosen from the further experiments were 1% of GA, 90°C and reaction time 15min.
27702505	5	46	theme	optimal	923:929	arg1	90°C					1005:1008	90°C	1005:1008	90°C	1005:1008	The optimal conjugation conditions chosen from the further experiments were 1% of GA, 90°C and reaction time 15min.
27702505	5	46	theme	optimal	923:929	arg1	GA					1001:1002	GA	1001:1002	GA	1001:1002	The optimal conjugation conditions chosen from the further experiments were 1% of GA, 90°C and reaction time 15min.
27702505	5	47	theme	conjugation	931:941	arg1	conditions					943:952	The optimal conjugation conditions	919:952	The optimal conjugation conditions chosen from the further experiments	919:988	The optimal conjugation conditions chosen from the further experiments were 1% of GA, 90°C and reaction time 15min.
27702505	5	47	theme	conjugation	931:941	arg1	%					996:996	1%	995:996	1% of GA, 90°C and reaction time 15min	995:1032	The optimal conjugation conditions chosen from the further experiments were 1% of GA, 90°C and reaction time 15min.
27702505	5	47	theme	conjugation	931:941	arg1	15min					1028:1032	reaction time 15min	1014:1032	reaction time 15min	1014:1032	The optimal conjugation conditions chosen from the further experiments were 1% of GA, 90°C and reaction time 15min.
27702505	5	47	theme	conjugation	931:941	arg1	90°C					1005:1008	90°C	1005:1008	90°C	1005:1008	The optimal conjugation conditions chosen from the further experiments were 1% of GA, 90°C and reaction time 15min.
27702505	5	47	theme	conjugation	931:941	arg1	GA					1001:1002	GA	1001:1002	GA	1001:1002	The optimal conjugation conditions chosen from the further experiments were 1% of GA, 90°C and reaction time 15min.
27702505	2	48	theme	high	567:570	arg1	chromatography					599:612	high performance size exclusion chromatography	567:612	high performance size exclusion chromatography	567:612	Upon decreasing of free amino group content in the glycosylated CPI to 72%, a new band near the loading end of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and a shift of CPI peak in high performance size exclusion chromatography confirmed that the covalent attachment of CPI to GA was successful.
27702505	5	49	theme	further	970:976	arg1	experiments					978:988	the further experiments	966:988	the further experiments	966:988	The optimal conjugation conditions chosen from the further experiments were 1% of GA, 90°C and reaction time 15min.
27702505	3	50	theme	grafted	741:747	arg1	CPI					749:751	grafted CPI	741:751	grafted CPI	741:751	The results of secondary structure analysis suggested that grafted CPI had decreased α-helix and β-sheet levels and increased random coils level.
27702505	2	51	from	content	407:413	arg1	CPI					435:437	the glycosylated CPI	418:437	the glycosylated CPI	418:437	Upon decreasing of free amino group content in the glycosylated CPI to 72%, a new band near the loading end of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and a shift of CPI peak in high performance size exclusion chromatography confirmed that the covalent attachment of CPI to GA was successful.
27702505	1	52	from	conjugate	191:199	arg1	solutions					269:277	aqueous solutions	261:277	aqueous solutions	261:277	The aim of this study was to produce covalently attached conjugate between canola protein isolate (CPI) and gum Arabic (GA) in aqueous solutions via the Maillard reaction at 90°C in a model system consisting of 2% CPI and 1, 2 or 4% GA.
27702505	1	53	dep	%	365:365	arg1	4					364:364	4	364:364	4	364:364	The aim of this study was to produce covalently attached conjugate between canola protein isolate (CPI) and gum Arabic (GA) in aqueous solutions via the Maillard reaction at 90°C in a model system consisting of 2% CPI and 1, 2 or 4% GA.
27702505	1	53	dep	%	365:365	arg1	2					359:359	2	359:359	2	359:359	The aim of this study was to produce covalently attached conjugate between canola protein isolate (CPI) and gum Arabic (GA) in aqueous solutions via the Maillard reaction at 90°C in a model system consisting of 2% CPI and 1, 2 or 4% GA.
27702505	1	54	theme	protein	216:222	arg1	CPI					233:235	CPI	233:235	CPI	233:235	The aim of this study was to produce covalently attached conjugate between canola protein isolate (CPI) and gum Arabic (GA) in aqueous solutions via the Maillard reaction at 90°C in a model system consisting of 2% CPI and 1, 2 or 4% GA.
27702505	1	54	theme	protein	216:222	arg1	isolate					224:230	canola protein isolate	209:230	canola protein isolate (CPI)	209:236	The aim of this study was to produce covalently attached conjugate between canola protein isolate (CPI) and gum Arabic (GA) in aqueous solutions via the Maillard reaction at 90°C in a model system consisting of 2% CPI and 1, 2 or 4% GA.
27702505	2	55	theme	amino	395:399	arg1	content					407:413	free amino group content	390:413	free amino group content in the glycosylated CPI	390:437	Upon decreasing of free amino group content in the glycosylated CPI to 72%, a new band near the loading end of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and a shift of CPI peak in high performance size exclusion chromatography confirmed that the covalent attachment of CPI to GA was successful.
27702505	3	56	theme	β-sheet	779:785	arg1	levels					787:792	α-helix and β-sheet levels	767:792	α-helix and β-sheet levels	767:792	The results of secondary structure analysis suggested that grafted CPI had decreased α-helix and β-sheet levels and increased random coils level.
27702505	2	57	theme	group	401:405	arg1	content					407:413	free amino group content	390:413	free amino group content in the glycosylated CPI	390:437	Upon decreasing of free amino group content in the glycosylated CPI to 72%, a new band near the loading end of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and a shift of CPI peak in high performance size exclusion chromatography confirmed that the covalent attachment of CPI to GA was successful.
27702505	2	58	from	chromatography	599:612	arg1	shift					546:550	a shift	544:550	a shift of CPI peak in high performance size exclusion chromatography	544:612	Upon decreasing of free amino group content in the glycosylated CPI to 72%, a new band near the loading end of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and a shift of CPI peak in high performance size exclusion chromatography confirmed that the covalent attachment of CPI to GA was successful.
27702505	2	58	from	chromatography	599:612	arg1	band					453:456	a new band	447:456	a new band near the loading end of sodium dodecyl sulfate-polyacrylamide gel electrophoresis	447:538	Upon decreasing of free amino group content in the glycosylated CPI to 72%, a new band near the loading end of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and a shift of CPI peak in high performance size exclusion chromatography confirmed that the covalent attachment of CPI to GA was successful.
27702505	0	59	theme	gum	63:65	arg1	Arabic					67:72	gum Arabic	63:72	gum Arabic	63:72	Preparation of chemically modified canola protein isolate with gum Arabic by means of Maillard reaction under wet-heating conditions.
27702505	1	60	theme	Maillard	287:294	arg1	reaction					296:303	the Maillard reaction	283:303	the Maillard reaction at 90°C in a model system consisting of 2% CPI and 1, 2 or 4% GA	283:368	The aim of this study was to produce covalently attached conjugate between canola protein isolate (CPI) and gum Arabic (GA) in aqueous solutions via the Maillard reaction at 90°C in a model system consisting of 2% CPI and 1, 2 or 4% GA.
27702505	2	61	from	shift	546:550	arg1	chromatography					599:612	high performance size exclusion chromatography	567:612	high performance size exclusion chromatography	567:612	Upon decreasing of free amino group content in the glycosylated CPI to 72%, a new band near the loading end of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and a shift of CPI peak in high performance size exclusion chromatography confirmed that the covalent attachment of CPI to GA was successful.
27702505	2	62	from	band	453:456	arg1	chromatography					599:612	high performance size exclusion chromatography	567:612	high performance size exclusion chromatography	567:612	Upon decreasing of free amino group content in the glycosylated CPI to 72%, a new band near the loading end of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and a shift of CPI peak in high performance size exclusion chromatography confirmed that the covalent attachment of CPI to GA was successful.
27702505	5	63	theme	reaction	1014:1021	arg1	15min					1028:1032	reaction time 15min	1014:1032	reaction time 15min	1014:1032	The optimal conjugation conditions chosen from the further experiments were 1% of GA, 90°C and reaction time 15min.
25129727	6	0	with	ISA	792:794	arg1	L					824:824	30, 60, 120 and 240 mg L(-1)	801:828	30, 60, 120 and 240 mg L(-1)	801:828	The treatments were applied as: foliar spray of deionized water only (control), seed soaked with ISA (90 mg L(-1)) and foliar spray of ISA with 30, 60, 120 and 240 mg L(-1).
25129727	7	1	from	L	871:871	arg1	spray					848:852	spray	848:852	spray of ISA at 120 mg L(-1)	848:875	The treatment 6 (spray of ISA at 120 mg L(-1)) showed the highest value for most of the parameters studied.
25129727	3	2	theme	EO	294:295	arg1	need					329:332	the need	325:332	the need of hour	325:340	Hence, escalated EO production of eucalyptus is the need of hour.
25129727	3	2	theme	EO	294:295	arg1	production					297:306	escalated EO production	284:306	escalated EO production of eucalyptus	284:320	Hence, escalated EO production of eucalyptus is the need of hour.
25129727	6	3	theme	mg	762:763	arg1	L					765:765	90 mg L(-1)	759:769	90 mg L(-1)	759:769	The treatments were applied as: foliar spray of deionized water only (control), seed soaked with ISA (90 mg L(-1)) and foliar spray of ISA with 30, 60, 120 and 240 mg L(-1).
25129727	6	3	theme	mg	762:763	arg1	ISA					754:756	ISA	754:756	ISA (90 mg L(-1))	754:770	The treatments were applied as: foliar spray of deionized water only (control), seed soaked with ISA (90 mg L(-1)) and foliar spray of ISA with 30, 60, 120 and 240 mg L(-1).
25129727	0	4	theme	essential	116:124	arg1	oil					126:128	essential oil	116:128	essential oil of Eucalyptus citriodora Hook	116:158	Radiolytically depolymerized sodium alginate improves physiological activities, yield attributes and composition of essential oil of Eucalyptus citriodora Hook.
25129727	5	5	theme	application	556:566	arg1	effect					539:544	the effect	535:544	the effect of foliar application of ISA on growth, biochemical, physiological, EO yield and composition of E. citriodora	535:654	A pot experiment was conducted to study the effect of foliar application of ISA on growth, biochemical, physiological, EO yield and composition of E. citriodora.
25129727	6	6	theme	ISA	792:794	arg1	L					765:765	90 mg L(-1)	759:769	90 mg L(-1)	759:769	The treatments were applied as: foliar spray of deionized water only (control), seed soaked with ISA (90 mg L(-1)) and foliar spray of ISA with 30, 60, 120 and 240 mg L(-1).
25129727	6	6	theme	ISA	792:794	arg1	ISA					754:756	ISA	754:756	ISA (90 mg L(-1))	754:770	The treatments were applied as: foliar spray of deionized water only (control), seed soaked with ISA (90 mg L(-1)) and foliar spray of ISA with 30, 60, 120 and 240 mg L(-1).
25129727	6	6	theme	ISA	792:794	arg1	spray					783:787	foliar spray	776:787	foliar spray of ISA with 30, 60, 120 and 240 mg L(-1)	776:828	The treatments were applied as: foliar spray of deionized water only (control), seed soaked with ISA (90 mg L(-1)) and foliar spray of ISA with 30, 60, 120 and 240 mg L(-1).
25129727	4	7	theme	sodium	472:477	arg1	ISA					489:491	ISA	489:491	ISA	489:491	Marine polysaccharides (sodium alginate) are processed through gamma radiation of particular intensity, to obtain the irradiated sodium alginate (ISA).
25129727	4	7	theme	sodium	472:477	arg1	alginate					479:486	the irradiated sodium alginate	457:486	the irradiated sodium alginate (ISA)	457:492	Marine polysaccharides (sodium alginate) are processed through gamma radiation of particular intensity, to obtain the irradiated sodium alginate (ISA).
25129727	4	8	theme	gamma	406:410	arg1	radiation					412:420	gamma radiation	406:420	gamma radiation of particular intensity	406:444	Marine polysaccharides (sodium alginate) are processed through gamma radiation of particular intensity, to obtain the irradiated sodium alginate (ISA).
25129727	2	9	dep	its	265:267	arg1	leaves					269:274	leaves	269:274	leaves	269:274	is highly valued for its citronellal-rich essential oil (EO) extracted from its leaves.
25129727	0	10	theme	oil	126:128	arg1	composition					101:111	composition	101:111	composition	101:111	Radiolytically depolymerized sodium alginate improves physiological activities, yield attributes and composition of essential oil of Eucalyptus citriodora Hook.
25129727	0	10	theme	oil	126:128	arg1	activities					68:77	physiological activities	54:77	physiological activities	54:77	Radiolytically depolymerized sodium alginate improves physiological activities, yield attributes and composition of essential oil of Eucalyptus citriodora Hook.
25129727	0	10	theme	oil	126:128	arg1	attributes					86:95	yield attributes	80:95	yield attributes	80:95	Radiolytically depolymerized sodium alginate improves physiological activities, yield attributes and composition of essential oil of Eucalyptus citriodora Hook.
25129727	7	11	theme	highest	889:895	arg1	value					897:901	the highest value	885:901	the highest value for most of the parameters studied	885:936	The treatment 6 (spray of ISA at 120 mg L(-1)) showed the highest value for most of the parameters studied.
25129727	5	12	theme	foliar	549:554	arg1	application					556:566	foliar application	549:566	foliar application of ISA	549:573	A pot experiment was conducted to study the effect of foliar application of ISA on growth, biochemical, physiological, EO yield and composition of E. citriodora.
25129727	6	13	dep	seed	737:740	arg1	soaked					742:747	soaked	742:747	seed soaked with ISA (90 mg L(-1)) and foliar spray of ISA with 30, 60, 120 and 240 mg L(-1)	737:828	The treatments were applied as: foliar spray of deionized water only (control), seed soaked with ISA (90 mg L(-1)) and foliar spray of ISA with 30, 60, 120 and 240 mg L(-1).
25129727	0	14	theme	depolymerized	15:27	arg1	alginate					36:43	Radiolytically depolymerized sodium alginate	0:43	Radiolytically depolymerized sodium alginate	0:43	Radiolytically depolymerized sodium alginate improves physiological activities, yield attributes and composition of essential oil of Eucalyptus citriodora Hook.
25129727	6	15	theme	foliar	776:781	arg1	spray					783:787	foliar spray	776:787	foliar spray of ISA with 30, 60, 120 and 240 mg L(-1)	776:828	The treatments were applied as: foliar spray of deionized water only (control), seed soaked with ISA (90 mg L(-1)) and foliar spray of ISA with 30, 60, 120 and 240 mg L(-1).
25129727	7	16	theme	mg	868:869	arg1	-1					873:874	-1	873:874	-1	873:874	The treatment 6 (spray of ISA at 120 mg L(-1)) showed the highest value for most of the parameters studied.
25129727	7	16	theme	mg	868:869	arg1	L					871:871	120 mg L	864:871	120 mg L(-1)	864:875	The treatment 6 (spray of ISA at 120 mg L(-1)) showed the highest value for most of the parameters studied.
25129727	4	17	theme	sodium	367:372	arg1	polysaccharides					350:364	Marine polysaccharides	343:364	Marine polysaccharides (sodium alginate)	343:382	Marine polysaccharides (sodium alginate) are processed through gamma radiation of particular intensity, to obtain the irradiated sodium alginate (ISA).
25129727	4	17	theme	sodium	367:372	arg1	alginate					374:381	sodium alginate	367:381	sodium alginate	367:381	Marine polysaccharides (sodium alginate) are processed through gamma radiation of particular intensity, to obtain the irradiated sodium alginate (ISA).
25129727	5	18	theme	pot	497:499	arg1	experiment					501:510	A pot experiment	495:510	A pot experiment	495:510	A pot experiment was conducted to study the effect of foliar application of ISA on growth, biochemical, physiological, EO yield and composition of E. citriodora.
25129727	8	19	theme	EO	960:961	arg1	yield					983:987	EO yield	980:987	EO yield (86.7%)	980:995	It also enhanced the EO content (33.3%), EO yield (86.7%), citronellal content (63.4%) and citronellal yield (205.5%) as compared to the control.
25129727	8	19	theme	EO	960:961	arg1	yield					1042:1046	citronellal yield	1030:1046	citronellal yield (205.5%)	1030:1055	It also enhanced the EO content (33.3%), EO yield (86.7%), citronellal content (63.4%) and citronellal yield (205.5%) as compared to the control.
25129727	8	19	theme	EO	960:961	arg1	content					963:969	the EO content	956:969	the EO content (33.3%)	956:977	It also enhanced the EO content (33.3%), EO yield (86.7%), citronellal content (63.4%) and citronellal yield (205.5%) as compared to the control.
25129727	8	19	theme	EO	960:961	arg1	%					976:976	33.3%	972:976	33.3%	972:976	It also enhanced the EO content (33.3%), EO yield (86.7%), citronellal content (63.4%) and citronellal yield (205.5%) as compared to the control.
25129727	8	19	theme	EO	960:961	arg1	content					1010:1016	citronellal content	998:1016	citronellal content (63.4%)	998:1024	It also enhanced the EO content (33.3%), EO yield (86.7%), citronellal content (63.4%) and citronellal yield (205.5%) as compared to the control.
25129727	5	20	theme	biochemical	586:596	arg1	yield					617:621	biochemical, physiological, EO yield	586:621	yield	617:621	A pot experiment was conducted to study the effect of foliar application of ISA on growth, biochemical, physiological, EO yield and composition of E. citriodora.
25129727	5	21	dep	yield	617:621	arg1	EO					614:615	biochemical, physiological, EO yield	586:621	EO	614:615	A pot experiment was conducted to study the effect of foliar application of ISA on growth, biochemical, physiological, EO yield and composition of E. citriodora.
25129727	3	22	theme	escalated	284:292	arg1	need					329:332	the need	325:332	the need of hour	325:340	Hence, escalated EO production of eucalyptus is the need of hour.
25129727	3	22	theme	escalated	284:292	arg1	production					297:306	escalated EO production	284:306	escalated EO production of eucalyptus	284:320	Hence, escalated EO production of eucalyptus is the need of hour.
25129727	5	23	theme	ISA	571:573	arg1	application					556:566	foliar application	549:566	foliar application of ISA	549:573	A pot experiment was conducted to study the effect of foliar application of ISA on growth, biochemical, physiological, EO yield and composition of E. citriodora.
25129727	5	24	from	effect	539:544	arg1	growth					578:583	growth	578:583	growth	578:583	A pot experiment was conducted to study the effect of foliar application of ISA on growth, biochemical, physiological, EO yield and composition of E. citriodora.
25129727	5	24	from	effect	539:544	arg1	composition					627:637	composition	627:637	composition	627:637	A pot experiment was conducted to study the effect of foliar application of ISA on growth, biochemical, physiological, EO yield and composition of E. citriodora.
25129727	5	24	from	effect	539:544	arg1	yield					617:621	biochemical, physiological, EO yield	586:621	yield	617:621	A pot experiment was conducted to study the effect of foliar application of ISA on growth, biochemical, physiological, EO yield and composition of E. citriodora.
25129727	0	25	theme	sodium	29:34	arg1	alginate					36:43	Radiolytically depolymerized sodium alginate	0:43	Radiolytically depolymerized sodium alginate	0:43	Radiolytically depolymerized sodium alginate improves physiological activities, yield attributes and composition of essential oil of Eucalyptus citriodora Hook.
25129727	0	26	theme	Eucalyptus	133:142	arg1	Hook					155:158	Eucalyptus citriodora Hook	133:158	Eucalyptus citriodora Hook	133:158	Radiolytically depolymerized sodium alginate improves physiological activities, yield attributes and composition of essential oil of Eucalyptus citriodora Hook.
25129727	8	27	theme	citronellal	1030:1040	arg1	yield					1042:1046	citronellal yield	1030:1046	citronellal yield (205.5%)	1030:1055	It also enhanced the EO content (33.3%), EO yield (86.7%), citronellal content (63.4%) and citronellal yield (205.5%) as compared to the control.
25129727	8	27	theme	citronellal	1030:1040	arg1	%					1054:1054	205.5%	1049:1054	205.5%	1049:1054	It also enhanced the EO content (33.3%), EO yield (86.7%), citronellal content (63.4%) and citronellal yield (205.5%) as compared to the control.
25129727	8	27	theme	citronellal	1030:1040	arg1	content					963:969	the EO content	956:969	the EO content (33.3%)	956:977	It also enhanced the EO content (33.3%), EO yield (86.7%), citronellal content (63.4%) and citronellal yield (205.5%) as compared to the control.
25129727	0	28	theme	physiological	54:66	arg1	activities					68:77	physiological activities	54:77	physiological activities	54:77	Radiolytically depolymerized sodium alginate improves physiological activities, yield attributes and composition of essential oil of Eucalyptus citriodora Hook.
25129727	6	29	theme	mg	821:822	arg1	L					824:824	30, 60, 120 and 240 mg L(-1)	801:828	30, 60, 120 and 240 mg L(-1)	801:828	The treatments were applied as: foliar spray of deionized water only (control), seed soaked with ISA (90 mg L(-1)) and foliar spray of ISA with 30, 60, 120 and 240 mg L(-1).
25129727	7	30	dep	treatment	835:843	arg1	spray					848:852	spray	848:852	spray of ISA at 120 mg L(-1)	848:875	The treatment 6 (spray of ISA at 120 mg L(-1)) showed the highest value for most of the parameters studied.
25129727	0	31	theme	Hook	155:158	arg1	oil					126:128	essential oil	116:128	essential oil of Eucalyptus citriodora Hook	116:158	Radiolytically depolymerized sodium alginate improves physiological activities, yield attributes and composition of essential oil of Eucalyptus citriodora Hook.
25129727	4	32	theme	irradiated	461:470	arg1	ISA					489:491	ISA	489:491	ISA	489:491	Marine polysaccharides (sodium alginate) are processed through gamma radiation of particular intensity, to obtain the irradiated sodium alginate (ISA).
25129727	4	32	theme	irradiated	461:470	arg1	alginate					479:486	the irradiated sodium alginate	457:486	the irradiated sodium alginate (ISA)	457:492	Marine polysaccharides (sodium alginate) are processed through gamma radiation of particular intensity, to obtain the irradiated sodium alginate (ISA).
25129727	4	33	theme	Marine	343:348	arg1	polysaccharides					350:364	Marine polysaccharides	343:364	Marine polysaccharides (sodium alginate)	343:382	Marine polysaccharides (sodium alginate) are processed through gamma radiation of particular intensity, to obtain the irradiated sodium alginate (ISA).
25129727	4	33	theme	Marine	343:348	arg1	alginate					374:381	sodium alginate	367:381	sodium alginate	367:381	Marine polysaccharides (sodium alginate) are processed through gamma radiation of particular intensity, to obtain the irradiated sodium alginate (ISA).
25129727	8	34	theme	citronellal	998:1008	arg1	%					1023:1023	63.4%	1019:1023	63.4%	1019:1023	It also enhanced the EO content (33.3%), EO yield (86.7%), citronellal content (63.4%) and citronellal yield (205.5%) as compared to the control.
25129727	8	34	theme	citronellal	998:1008	arg1	content					963:969	the EO content	956:969	the EO content (33.3%)	956:977	It also enhanced the EO content (33.3%), EO yield (86.7%), citronellal content (63.4%) and citronellal yield (205.5%) as compared to the control.
25129727	8	34	theme	citronellal	998:1008	arg1	content					1010:1016	citronellal content	998:1016	citronellal content (63.4%)	998:1024	It also enhanced the EO content (33.3%), EO yield (86.7%), citronellal content (63.4%) and citronellal yield (205.5%) as compared to the control.
25129727	8	35	theme	EO	980:981	arg1	yield					983:987	EO yield	980:987	EO yield (86.7%)	980:995	It also enhanced the EO content (33.3%), EO yield (86.7%), citronellal content (63.4%) and citronellal yield (205.5%) as compared to the control.
25129727	8	35	theme	EO	980:981	arg1	%					994:994	86.7%	990:994	86.7%	990:994	It also enhanced the EO content (33.3%), EO yield (86.7%), citronellal content (63.4%) and citronellal yield (205.5%) as compared to the control.
25129727	8	35	theme	EO	980:981	arg1	content					963:969	the EO content	956:969	the EO content (33.3%)	956:977	It also enhanced the EO content (33.3%), EO yield (86.7%), citronellal content (63.4%) and citronellal yield (205.5%) as compared to the control.
25129727	3	36	theme	eucalyptus	311:320	arg1	need					329:332	the need	325:332	the need of hour	325:340	Hence, escalated EO production of eucalyptus is the need of hour.
25129727	3	36	theme	eucalyptus	311:320	arg1	production					297:306	escalated EO production	284:306	escalated EO production of eucalyptus	284:320	Hence, escalated EO production of eucalyptus is the need of hour.
25129727	0	37	theme	yield	80:84	arg1	attributes					86:95	yield attributes	80:95	yield attributes	80:95	Radiolytically depolymerized sodium alginate improves physiological activities, yield attributes and composition of essential oil of Eucalyptus citriodora Hook.
25129727	0	38	theme	citriodora	144:153	arg1	Hook					155:158	Eucalyptus citriodora Hook	133:158	Eucalyptus citriodora Hook	133:158	Radiolytically depolymerized sodium alginate improves physiological activities, yield attributes and composition of essential oil of Eucalyptus citriodora Hook.
25129727	4	39	theme	particular	425:434	arg1	intensity					436:444	particular intensity	425:444	particular intensity	425:444	Marine polysaccharides (sodium alginate) are processed through gamma radiation of particular intensity, to obtain the irradiated sodium alginate (ISA).
25129727	6	40	theme	water	715:719	arg1	spray					696:700	foliar spray	689:700	foliar spray	689:700	The treatments were applied as: foliar spray of deionized water only (control), seed soaked with ISA (90 mg L(-1)) and foliar spray of ISA with 30, 60, 120 and 240 mg L(-1).
25129727	7	41	theme	ISA	857:859	arg1	spray					848:852	spray	848:852	spray of ISA at 120 mg L(-1)	848:875	The treatment 6 (spray of ISA at 120 mg L(-1)) showed the highest value for most of the parameters studied.
25129727	2	42	theme	essential	231:239	arg1	EO					246:247	EO	246:247	EO	246:247	is highly valued for its citronellal-rich essential oil (EO) extracted from its leaves.
25129727	2	42	theme	essential	231:239	arg1	oil					241:243	its citronellal-rich essential oil	210:243	its citronellal-rich essential oil (EO) extracted from its leaves	210:274	is highly valued for its citronellal-rich essential oil (EO) extracted from its leaves.
25129727	6	43	theme	deionized	705:713	arg1	water					715:719	deionized water	705:719	deionized water only (control)	705:734	The treatments were applied as: foliar spray of deionized water only (control), seed soaked with ISA (90 mg L(-1)) and foliar spray of ISA with 30, 60, 120 and 240 mg L(-1).
25129727	6	43	theme	deionized	705:713	arg1	control					727:733	control	727:733	control	727:733	The treatments were applied as: foliar spray of deionized water only (control), seed soaked with ISA (90 mg L(-1)) and foliar spray of ISA with 30, 60, 120 and 240 mg L(-1).
25129727	5	44	dep	biochemical	586:596	arg1	physiological					599:611	physiological	599:611	physiological	599:611	A pot experiment was conducted to study the effect of foliar application of ISA on growth, biochemical, physiological, EO yield and composition of E. citriodora.
25129727	2	45	theme	citronellal-rich	214:229	arg1	EO					246:247	EO	246:247	EO	246:247	is highly valued for its citronellal-rich essential oil (EO) extracted from its leaves.
25129727	2	45	theme	citronellal-rich	214:229	arg1	oil					241:243	its citronellal-rich essential oil	210:243	its citronellal-rich essential oil (EO) extracted from its leaves	210:274	is highly valued for its citronellal-rich essential oil (EO) extracted from its leaves.
25129727	4	46	theme	intensity	436:444	arg1	radiation					412:420	gamma radiation	406:420	gamma radiation of particular intensity	406:444	Marine polysaccharides (sodium alginate) are processed through gamma radiation of particular intensity, to obtain the irradiated sodium alginate (ISA).
25129727	1	47	theme	Eucalyptus	161:170	arg1	Hook					183:186	Eucalyptus citriodora Hook	161:186	Eucalyptus citriodora Hook.	161:187	Eucalyptus citriodora Hook.
25129727	3	48	theme	hour	337:340	arg1	need					329:332	the need	325:332	the need of hour	325:340	Hence, escalated EO production of eucalyptus is the need of hour.
25129727	3	48	theme	hour	337:340	arg1	production					297:306	escalated EO production	284:306	escalated EO production of eucalyptus	284:320	Hence, escalated EO production of eucalyptus is the need of hour.
25129727	6	49	theme	foliar	689:694	arg1	spray					696:700	foliar spray	689:700	foliar spray	689:700	The treatments were applied as: foliar spray of deionized water only (control), seed soaked with ISA (90 mg L(-1)) and foliar spray of ISA with 30, 60, 120 and 240 mg L(-1).
25129727	1	50	theme	citriodora	172:181	arg1	Hook					183:186	Eucalyptus citriodora Hook	161:186	Eucalyptus citriodora Hook.	161:187	Eucalyptus citriodora Hook.
25129727	5	51	theme	citriodora	645:654	arg1	growth					578:583	growth	578:583	growth	578:583	A pot experiment was conducted to study the effect of foliar application of ISA on growth, biochemical, physiological, EO yield and composition of E. citriodora.
25129727	5	51	theme	citriodora	645:654	arg1	composition					627:637	composition	627:637	composition	627:637	A pot experiment was conducted to study the effect of foliar application of ISA on growth, biochemical, physiological, EO yield and composition of E. citriodora.
25129727	5	51	theme	citriodora	645:654	arg1	yield					617:621	biochemical, physiological, EO yield	586:621	yield	617:621	A pot experiment was conducted to study the effect of foliar application of ISA on growth, biochemical, physiological, EO yield and composition of E. citriodora.
26002148	0	0	theme	silica	83:88	arg1	characteristics					31:45	Efficient and rapid adsorption characteristics	0:45	Efficient and rapid adsorption characteristics of templating modified guar gum and silica	0:88	Efficient and rapid adsorption characteristics of templating modified guar gum and silica nanocomposite toward removal of toxic reactive blue and Congo red dyes.
26002148	1	1	theme	toxic	332:336	arg1	dyes					378:381	toxic reactive blue 4 (RB) and Congo red (CR) dyes	332:381	toxic reactive blue 4 (RB) and Congo red (CR) dyes from aqueous solution	332:403	The present study highlights the potentiality of sol-gel synthesized guar gum-graft-poly (acrylamide)/silica (g-GG/SiO2) hybrid nanocomposite toward the rapid removal of toxic reactive blue 4 (RB) and Congo red (CR) dyes from aqueous solution.
26002148	4	2	theme	driven	794:799	arg1	process					812:818	the pH driven adsorption process	787:818	the pH driven adsorption process	787:818	Here, the pH driven adsorption process depends strongly on the ionic strength of the salt solution.
26002148	2	3	theme	nanoscale	565:573	arg1	particles					582:590	homogeneous nanoscale silica particles	553:590	homogeneous nanoscale silica particles	553:590	Various physicochemical characterizations support the feasibility of the functionalized guar gum matrix as efficient template for the formation of homogeneous nanoscale silica particles.
26002148	1	4	theme	acrylamide	252:261	arg1	nanocomposite					290:302	sol-gel synthesized guar gum-graft-poly (acrylamide)/silica (g-GG/SiO2) hybrid nanocomposite	211:302	sol-gel synthesized guar gum-graft-poly (acrylamide)/silica (g-GG/SiO2) hybrid nanocomposite	211:302	The present study highlights the potentiality of sol-gel synthesized guar gum-graft-poly (acrylamide)/silica (g-GG/SiO2) hybrid nanocomposite toward the rapid removal of toxic reactive blue 4 (RB) and Congo red (CR) dyes from aqueous solution.
26002148	4	5	theme	solution	871:878	arg1	strength					850:857	the ionic strength	840:857	the ionic strength of the salt solution	840:878	Here, the pH driven adsorption process depends strongly on the ionic strength of the salt solution.
26002148	1	6	theme	reactive	338:345	arg1	dyes					378:381	toxic reactive blue 4 (RB) and Congo red (CR) dyes	332:381	toxic reactive blue 4 (RB) and Congo red (CR) dyes from aqueous solution	332:403	The present study highlights the potentiality of sol-gel synthesized guar gum-graft-poly (acrylamide)/silica (g-GG/SiO2) hybrid nanocomposite toward the rapid removal of toxic reactive blue 4 (RB) and Congo red (CR) dyes from aqueous solution.
26002148	1	7	from	removal	321:327	arg1	solution					396:403	aqueous solution	388:403	aqueous solution	388:403	The present study highlights the potentiality of sol-gel synthesized guar gum-graft-poly (acrylamide)/silica (g-GG/SiO2) hybrid nanocomposite toward the rapid removal of toxic reactive blue 4 (RB) and Congo red (CR) dyes from aqueous solution.
26002148	1	8	theme	aqueous	388:394	arg1	solution					396:403	aqueous solution	388:403	aqueous solution	388:403	The present study highlights the potentiality of sol-gel synthesized guar gum-graft-poly (acrylamide)/silica (g-GG/SiO2) hybrid nanocomposite toward the rapid removal of toxic reactive blue 4 (RB) and Congo red (CR) dyes from aqueous solution.
26002148	1	9	from	solution	396:403	arg1	dyes					378:381	toxic reactive blue 4 (RB) and Congo red (CR) dyes	332:381	toxic reactive blue 4 (RB) and Congo red (CR) dyes from aqueous solution	332:403	The present study highlights the potentiality of sol-gel synthesized guar gum-graft-poly (acrylamide)/silica (g-GG/SiO2) hybrid nanocomposite toward the rapid removal of toxic reactive blue 4 (RB) and Congo red (CR) dyes from aqueous solution.
26002148	1	9	from	solution	396:403	arg1	removal					321:327	the rapid removal	311:327	the rapid removal of toxic reactive blue 4 (RB) and Congo red (CR) dyes from aqueous solution	311:403	The present study highlights the potentiality of sol-gel synthesized guar gum-graft-poly (acrylamide)/silica (g-GG/SiO2) hybrid nanocomposite toward the rapid removal of toxic reactive blue 4 (RB) and Congo red (CR) dyes from aqueous solution.
26002148	4	10	theme	ionic	844:848	arg1	strength					850:857	the ionic strength	840:857	the ionic strength of the salt solution	840:878	Here, the pH driven adsorption process depends strongly on the ionic strength of the salt solution.
26002148	1	11	theme	blue	347:350	arg1	dyes					378:381	toxic reactive blue 4 (RB) and Congo red (CR) dyes	332:381	toxic reactive blue 4 (RB) and Congo red (CR) dyes from aqueous solution	332:403	The present study highlights the potentiality of sol-gel synthesized guar gum-graft-poly (acrylamide)/silica (g-GG/SiO2) hybrid nanocomposite toward the rapid removal of toxic reactive blue 4 (RB) and Congo red (CR) dyes from aqueous solution.
26002148	2	12	theme	particles	582:590	arg1	formation					540:548	the formation	536:548	the formation of homogeneous nanoscale silica particles	536:590	Various physicochemical characterizations support the feasibility of the functionalized guar gum matrix as efficient template for the formation of homogeneous nanoscale silica particles.
26002148	3	13	dep	dyes	750:753	arg1	g					729:729	Qmax: 233.24 mg g(-1)	713:733	Qmax: 233.24 mg g(-1)	713:733	The composite demonstrates rapid and superior adsorption efficiency of RB (Qmax: 579.01 mg g(-1) within 40 min) and CR (Qmax: 233.24 mg g(-1) within 30 min) dyes from aqueous environment.
26002148	3	14	from	environment	768:778	arg1	efficiency					650:659	rapid and superior adsorption efficiency	620:659	rapid and superior adsorption efficiency of RB (Qmax: 579.01 mg g(-1) within 40 min) and CR (Qmax: 233.24 mg g(-1) within 30 min) dyes from aqueous environment	620:778	The composite demonstrates rapid and superior adsorption efficiency of RB (Qmax: 579.01 mg g(-1) within 40 min) and CR (Qmax: 233.24 mg g(-1) within 30 min) dyes from aqueous environment.
26002148	3	14	from	environment	768:778	arg1	dyes					750:753	RB (Qmax: 579.01 mg g(-1) within 40 min) and CR (Qmax: 233.24 mg g(-1) within 30 min) dyes	664:753	dyes	750:753	The composite demonstrates rapid and superior adsorption efficiency of RB (Qmax: 579.01 mg g(-1) within 40 min) and CR (Qmax: 233.24 mg g(-1) within 30 min) dyes from aqueous environment.
26002148	3	14	from	environment	768:778	arg1	RB					664:665	RB (Qmax: 579.01 mg g(-1) within 40 min) and CR (Qmax: 233.24 mg g(-1) within 30 min) dyes	664:753	RB	664:665	The composite demonstrates rapid and superior adsorption efficiency of RB (Qmax: 579.01 mg g(-1) within 40 min) and CR (Qmax: 233.24 mg g(-1) within 30 min) dyes from aqueous environment.
26002148	1	15	theme	/silica	263:269	arg1	nanocomposite					290:302	sol-gel synthesized guar gum-graft-poly (acrylamide)/silica (g-GG/SiO2) hybrid nanocomposite	211:302	sol-gel synthesized guar gum-graft-poly (acrylamide)/silica (g-GG/SiO2) hybrid nanocomposite	211:302	The present study highlights the potentiality of sol-gel synthesized guar gum-graft-poly (acrylamide)/silica (g-GG/SiO2) hybrid nanocomposite toward the rapid removal of toxic reactive blue 4 (RB) and Congo red (CR) dyes from aqueous solution.
26002148	2	16	theme	matrix	503:508	arg1	feasibility					460:470	the feasibility	456:470	the feasibility of the functionalized guar gum matrix	456:508	Various physicochemical characterizations support the feasibility of the functionalized guar gum matrix as efficient template for the formation of homogeneous nanoscale silica particles.
26002148	3	17	from	efficiency	650:659	arg1	environment					768:778	aqueous environment	760:778	aqueous environment	760:778	The composite demonstrates rapid and superior adsorption efficiency of RB (Qmax: 579.01 mg g(-1) within 40 min) and CR (Qmax: 233.24 mg g(-1) within 30 min) dyes from aqueous environment.
26002148	2	18	theme	silica	575:580	arg1	particles					582:590	homogeneous nanoscale silica particles	553:590	homogeneous nanoscale silica particles	553:590	Various physicochemical characterizations support the feasibility of the functionalized guar gum matrix as efficient template for the formation of homogeneous nanoscale silica particles.
26002148	2	19	theme	gum	499:501	arg1	matrix					503:508	the functionalized guar gum matrix	475:508	the functionalized guar gum matrix	475:508	Various physicochemical characterizations support the feasibility of the functionalized guar gum matrix as efficient template for the formation of homogeneous nanoscale silica particles.
26002148	7	20	theme	nanocomposite	1247:1259	arg1	efficacy					1235:1242	the excellent regenerative efficacy	1208:1242	the excellent regenerative efficacy of nanocomposite	1208:1259	Desorption study predicts the excellent regenerative efficacy of nanocomposite.
26002148	2	21	theme	guar	494:497	arg1	gum					499:501	the functionalized guar gum	475:501	the functionalized guar gum matrix	475:508	Various physicochemical characterizations support the feasibility of the functionalized guar gum matrix as efficient template for the formation of homogeneous nanoscale silica particles.
26002148	3	22	dep	RB	664:665	arg1	g					684:684	Qmax: 579.01 mg g(-1)	668:688	Qmax: 579.01 mg g(-1)	668:688	The composite demonstrates rapid and superior adsorption efficiency of RB (Qmax: 579.01 mg g(-1) within 40 min) and CR (Qmax: 233.24 mg g(-1) within 30 min) dyes from aqueous environment.
26002148	4	23	dep	driven	794:799	arg1	pH					791:792	pH	791:792	pH	791:792	Here, the pH driven adsorption process depends strongly on the ionic strength of the salt solution.
26002148	1	24	theme	present	166:172	arg1	study					174:178	The present study	162:178	The present study	162:178	The present study highlights the potentiality of sol-gel synthesized guar gum-graft-poly (acrylamide)/silica (g-GG/SiO2) hybrid nanocomposite toward the rapid removal of toxic reactive blue 4 (RB) and Congo red (CR) dyes from aqueous solution.
26002148	2	25	theme	Various	406:412	arg1	characterizations					430:446	Various physicochemical characterizations	406:446	Various physicochemical characterizations	406:446	Various physicochemical characterizations support the feasibility of the functionalized guar gum matrix as efficient template for the formation of homogeneous nanoscale silica particles.
26002148	6	26	theme	process	1173:1179	arg1	nature					1148:1153	spontaneous nature	1136:1153	spontaneous nature of the adsorption process	1136:1179	The adsorption equilibrium is in good agreement with the Langmuir isotherm, while the thermodynamics study confirms spontaneous nature of the adsorption process.
26002148	3	27	theme	RB	664:665	arg1	efficiency					650:659	rapid and superior adsorption efficiency	620:659	rapid and superior adsorption efficiency of RB (Qmax: 579.01 mg g(-1) within 40 min) and CR (Qmax: 233.24 mg g(-1) within 30 min) dyes from aqueous environment	620:778	The composite demonstrates rapid and superior adsorption efficiency of RB (Qmax: 579.01 mg g(-1) within 40 min) and CR (Qmax: 233.24 mg g(-1) within 30 min) dyes from aqueous environment.
26002148	3	28	theme	mg	681:682	arg1	g					684:684	Qmax: 579.01 mg g(-1)	668:688	Qmax: 579.01 mg g(-1)	668:688	The composite demonstrates rapid and superior adsorption efficiency of RB (Qmax: 579.01 mg g(-1) within 40 min) and CR (Qmax: 233.24 mg g(-1) within 30 min) dyes from aqueous environment.
26002148	0	29	theme	Efficient	0:8	arg1	characteristics					31:45	Efficient and rapid adsorption characteristics	0:45	Efficient and rapid adsorption characteristics of templating modified guar gum and silica	0:88	Efficient and rapid adsorption characteristics of templating modified guar gum and silica nanocomposite toward removal of toxic reactive blue and Congo red dyes.
26002148	1	30	theme	g-GG/SiO2	272:280	arg1	nanocomposite					290:302	sol-gel synthesized guar gum-graft-poly (acrylamide)/silica (g-GG/SiO2) hybrid nanocomposite	211:302	sol-gel synthesized guar gum-graft-poly (acrylamide)/silica (g-GG/SiO2) hybrid nanocomposite	211:302	The present study highlights the potentiality of sol-gel synthesized guar gum-graft-poly (acrylamide)/silica (g-GG/SiO2) hybrid nanocomposite toward the rapid removal of toxic reactive blue 4 (RB) and Congo red (CR) dyes from aqueous solution.
26002148	6	31	theme	adsorption	1162:1171	arg1	process					1173:1179	the adsorption process	1158:1179	the adsorption process	1158:1179	The adsorption equilibrium is in good agreement with the Langmuir isotherm, while the thermodynamics study confirms spontaneous nature of the adsorption process.
26002148	3	32	theme	Qmax	668:671	arg1	g					684:684	Qmax: 579.01 mg g(-1)	668:688	Qmax: 579.01 mg g(-1)	668:688	The composite demonstrates rapid and superior adsorption efficiency of RB (Qmax: 579.01 mg g(-1) within 40 min) and CR (Qmax: 233.24 mg g(-1) within 30 min) dyes from aqueous environment.
26002148	0	33	theme	adsorption	20:29	arg1	characteristics					31:45	Efficient and rapid adsorption characteristics	0:45	Efficient and rapid adsorption characteristics of templating modified guar gum and silica	0:88	Efficient and rapid adsorption characteristics of templating modified guar gum and silica nanocomposite toward removal of toxic reactive blue and Congo red dyes.
26002148	1	34	dep	blue	347:350	arg1	RB					355:356	RB	355:356	RB	355:356	The present study highlights the potentiality of sol-gel synthesized guar gum-graft-poly (acrylamide)/silica (g-GG/SiO2) hybrid nanocomposite toward the rapid removal of toxic reactive blue 4 (RB) and Congo red (CR) dyes from aqueous solution.
26002148	1	34	dep	blue	347:350	arg1	4					352:352	4	352:352	4	352:352	The present study highlights the potentiality of sol-gel synthesized guar gum-graft-poly (acrylamide)/silica (g-GG/SiO2) hybrid nanocomposite toward the rapid removal of toxic reactive blue 4 (RB) and Congo red (CR) dyes from aqueous solution.
26002148	0	35	theme	reactive	128:135	arg1	dyes					156:159	toxic reactive blue and Congo red dyes	122:159	toxic reactive blue and Congo red dyes	122:159	Efficient and rapid adsorption characteristics of templating modified guar gum and silica nanocomposite toward removal of toxic reactive blue and Congo red dyes.
26002148	0	36	theme	rapid	14:18	arg1	characteristics					31:45	Efficient and rapid adsorption characteristics	0:45	Efficient and rapid adsorption characteristics of templating modified guar gum and silica	0:88	Efficient and rapid adsorption characteristics of templating modified guar gum and silica nanocomposite toward removal of toxic reactive blue and Congo red dyes.
26002148	1	37	theme	hybrid	283:288	arg1	nanocomposite					290:302	sol-gel synthesized guar gum-graft-poly (acrylamide)/silica (g-GG/SiO2) hybrid nanocomposite	211:302	sol-gel synthesized guar gum-graft-poly (acrylamide)/silica (g-GG/SiO2) hybrid nanocomposite	211:302	The present study highlights the potentiality of sol-gel synthesized guar gum-graft-poly (acrylamide)/silica (g-GG/SiO2) hybrid nanocomposite toward the rapid removal of toxic reactive blue 4 (RB) and Congo red (CR) dyes from aqueous solution.
26002148	0	38	theme	toxic	122:126	arg1	dyes					156:159	toxic reactive blue and Congo red dyes	122:159	toxic reactive blue and Congo red dyes	122:159	Efficient and rapid adsorption characteristics of templating modified guar gum and silica nanocomposite toward removal of toxic reactive blue and Congo red dyes.
26002148	6	39	theme	good	1053:1056	arg1	agreement					1058:1066	good agreement	1053:1066	good agreement with the Langmuir isotherm	1053:1093	The adsorption equilibrium is in good agreement with the Langmuir isotherm, while the thermodynamics study confirms spontaneous nature of the adsorption process.
26002148	6	40	theme	thermodynamics	1106:1119	arg1	study					1121:1125	the thermodynamics study	1102:1125	the thermodynamics study	1102:1125	The adsorption equilibrium is in good agreement with the Langmuir isotherm, while the thermodynamics study confirms spontaneous nature of the adsorption process.
26002148	4	41	theme	salt	866:869	arg1	solution					871:878	the salt solution	862:878	the salt solution	862:878	Here, the pH driven adsorption process depends strongly on the ionic strength of the salt solution.
26002148	1	42	theme	nanocomposite	290:302	arg1	potentiality					195:206	the potentiality	191:206	the potentiality of sol-gel synthesized guar gum-graft-poly (acrylamide)/silica (g-GG/SiO2) hybrid nanocomposite toward the rapid removal of toxic reactive blue 4 (RB) and Congo red (CR) dyes from aqueous solution	191:403	The present study highlights the potentiality of sol-gel synthesized guar gum-graft-poly (acrylamide)/silica (g-GG/SiO2) hybrid nanocomposite toward the rapid removal of toxic reactive blue 4 (RB) and Congo red (CR) dyes from aqueous solution.
26002148	5	43	theme	intraparticle	969:981	arg1	diffusion					983:991	intraparticle diffusion	969:991	intraparticle diffusion	969:991	The adsorption kinetics data predicts that pseudo second-order (surface adsorption) and intraparticle diffusion take place simultaneously.
26002148	3	44	theme	dyes	750:753	arg1	efficiency					650:659	rapid and superior adsorption efficiency	620:659	rapid and superior adsorption efficiency of RB (Qmax: 579.01 mg g(-1) within 40 min) and CR (Qmax: 233.24 mg g(-1) within 30 min) dyes from aqueous environment	620:778	The composite demonstrates rapid and superior adsorption efficiency of RB (Qmax: 579.01 mg g(-1) within 40 min) and CR (Qmax: 233.24 mg g(-1) within 30 min) dyes from aqueous environment.
26002148	7	45	theme	regenerative	1222:1233	arg1	efficacy					1235:1242	the excellent regenerative efficacy	1208:1242	the excellent regenerative efficacy of nanocomposite	1208:1259	Desorption study predicts the excellent regenerative efficacy of nanocomposite.
26002148	3	46	theme	CR	709:710	arg1	dyes					750:753	RB (Qmax: 579.01 mg g(-1) within 40 min) and CR (Qmax: 233.24 mg g(-1) within 30 min) dyes	664:753	dyes	750:753	The composite demonstrates rapid and superior adsorption efficiency of RB (Qmax: 579.01 mg g(-1) within 40 min) and CR (Qmax: 233.24 mg g(-1) within 30 min) dyes from aqueous environment.
26002148	6	47	with	agreement	1058:1066	arg1	isotherm					1086:1093	the Langmuir isotherm	1073:1093	the Langmuir isotherm	1073:1093	The adsorption equilibrium is in good agreement with the Langmuir isotherm, while the thermodynamics study confirms spontaneous nature of the adsorption process.
26002148	0	48	theme	blue	137:140	arg1	dyes					156:159	toxic reactive blue and Congo red dyes	122:159	toxic reactive blue and Congo red dyes	122:159	Efficient and rapid adsorption characteristics of templating modified guar gum and silica nanocomposite toward removal of toxic reactive blue and Congo red dyes.
26002148	1	49	theme	red	369:371	arg1	dyes					378:381	toxic reactive blue 4 (RB) and Congo red (CR) dyes	332:381	toxic reactive blue 4 (RB) and Congo red (CR) dyes from aqueous solution	332:403	The present study highlights the potentiality of sol-gel synthesized guar gum-graft-poly (acrylamide)/silica (g-GG/SiO2) hybrid nanocomposite toward the rapid removal of toxic reactive blue 4 (RB) and Congo red (CR) dyes from aqueous solution.
26002148	0	50	dep	templating	50:59	arg1	gum					75:77	modified guar gum	61:77	modified guar gum	61:77	Efficient and rapid adsorption characteristics of templating modified guar gum and silica nanocomposite toward removal of toxic reactive blue and Congo red dyes.
26002148	2	51	theme	efficient	513:521	arg1	template					523:530	efficient template	513:530	efficient template for the formation of homogeneous nanoscale silica particles	513:590	Various physicochemical characterizations support the feasibility of the functionalized guar gum matrix as efficient template for the formation of homogeneous nanoscale silica particles.
26002148	1	52	theme	sol-gel	211:217	arg1	nanocomposite					290:302	sol-gel synthesized guar gum-graft-poly (acrylamide)/silica (g-GG/SiO2) hybrid nanocomposite	211:302	sol-gel synthesized guar gum-graft-poly (acrylamide)/silica (g-GG/SiO2) hybrid nanocomposite	211:302	The present study highlights the potentiality of sol-gel synthesized guar gum-graft-poly (acrylamide)/silica (g-GG/SiO2) hybrid nanocomposite toward the rapid removal of toxic reactive blue 4 (RB) and Congo red (CR) dyes from aqueous solution.
26002148	0	53	theme	modified	61:68	arg1	gum					75:77	modified guar gum	61:77	modified guar gum	61:77	Efficient and rapid adsorption characteristics of templating modified guar gum and silica nanocomposite toward removal of toxic reactive blue and Congo red dyes.
26002148	0	54	theme	red	152:154	arg1	dyes					156:159	toxic reactive blue and Congo red dyes	122:159	toxic reactive blue and Congo red dyes	122:159	Efficient and rapid adsorption characteristics of templating modified guar gum and silica nanocomposite toward removal of toxic reactive blue and Congo red dyes.
26002148	3	55	theme	adsorption	639:648	arg1	efficiency					650:659	rapid and superior adsorption efficiency	620:659	rapid and superior adsorption efficiency of RB (Qmax: 579.01 mg g(-1) within 40 min) and CR (Qmax: 233.24 mg g(-1) within 30 min) dyes from aqueous environment	620:778	The composite demonstrates rapid and superior adsorption efficiency of RB (Qmax: 579.01 mg g(-1) within 40 min) and CR (Qmax: 233.24 mg g(-1) within 30 min) dyes from aqueous environment.
26002148	5	56	theme	pseudo	924:929	arg1	adsorption					953:962	surface adsorption	945:962	surface adsorption	945:962	The adsorption kinetics data predicts that pseudo second-order (surface adsorption) and intraparticle diffusion take place simultaneously.
26002148	5	56	theme	pseudo	924:929	arg1	second-order					931:942	pseudo second-order	924:942	pseudo second-order (surface adsorption)	924:963	The adsorption kinetics data predicts that pseudo second-order (surface adsorption) and intraparticle diffusion take place simultaneously.
26002148	0	57	theme	templating	50:59	arg1	characteristics					31:45	Efficient and rapid adsorption characteristics	0:45	Efficient and rapid adsorption characteristics of templating modified guar gum and silica	0:88	Efficient and rapid adsorption characteristics of templating modified guar gum and silica nanocomposite toward removal of toxic reactive blue and Congo red dyes.
26002148	1	58	theme	rapid	315:319	arg1	removal					321:327	the rapid removal	311:327	the rapid removal of toxic reactive blue 4 (RB) and Congo red (CR) dyes from aqueous solution	311:403	The present study highlights the potentiality of sol-gel synthesized guar gum-graft-poly (acrylamide)/silica (g-GG/SiO2) hybrid nanocomposite toward the rapid removal of toxic reactive blue 4 (RB) and Congo red (CR) dyes from aqueous solution.
26002148	2	59	theme	homogeneous	553:563	arg1	particles					582:590	homogeneous nanoscale silica particles	553:590	homogeneous nanoscale silica particles	553:590	Various physicochemical characterizations support the feasibility of the functionalized guar gum matrix as efficient template for the formation of homogeneous nanoscale silica particles.
26002148	2	60	theme	physicochemical	414:428	arg1	characterizations					430:446	Various physicochemical characterizations	406:446	Various physicochemical characterizations	406:446	Various physicochemical characterizations support the feasibility of the functionalized guar gum matrix as efficient template for the formation of homogeneous nanoscale silica particles.
26002148	6	61	theme	spontaneous	1136:1146	arg1	nature					1148:1153	spontaneous nature	1136:1153	spontaneous nature of the adsorption process	1136:1179	The adsorption equilibrium is in good agreement with the Langmuir isotherm, while the thermodynamics study confirms spontaneous nature of the adsorption process.
26002148	3	62	theme	superior	630:637	arg1	efficiency					650:659	rapid and superior adsorption efficiency	620:659	rapid and superior adsorption efficiency of RB (Qmax: 579.01 mg g(-1) within 40 min) and CR (Qmax: 233.24 mg g(-1) within 30 min) dyes from aqueous environment	620:778	The composite demonstrates rapid and superior adsorption efficiency of RB (Qmax: 579.01 mg g(-1) within 40 min) and CR (Qmax: 233.24 mg g(-1) within 30 min) dyes from aqueous environment.
26002148	3	63	theme	Qmax	713:716	arg1	g					729:729	Qmax: 233.24 mg g(-1)	713:733	Qmax: 233.24 mg g(-1)	713:733	The composite demonstrates rapid and superior adsorption efficiency of RB (Qmax: 579.01 mg g(-1) within 40 min) and CR (Qmax: 233.24 mg g(-1) within 30 min) dyes from aqueous environment.
26002148	4	64	theme	adsorption	801:810	arg1	process					812:818	the pH driven adsorption process	787:818	the pH driven adsorption process	787:818	Here, the pH driven adsorption process depends strongly on the ionic strength of the salt solution.
26002148	5	65	theme	surface	945:951	arg1	adsorption					953:962	surface adsorption	945:962	surface adsorption	945:962	The adsorption kinetics data predicts that pseudo second-order (surface adsorption) and intraparticle diffusion take place simultaneously.
26002148	5	65	theme	surface	945:951	arg1	second-order					931:942	pseudo second-order	924:942	pseudo second-order (surface adsorption)	924:963	The adsorption kinetics data predicts that pseudo second-order (surface adsorption) and intraparticle diffusion take place simultaneously.
26002148	3	66	theme	rapid	620:624	arg1	efficiency					650:659	rapid and superior adsorption efficiency	620:659	rapid and superior adsorption efficiency of RB (Qmax: 579.01 mg g(-1) within 40 min) and CR (Qmax: 233.24 mg g(-1) within 30 min) dyes from aqueous environment	620:778	The composite demonstrates rapid and superior adsorption efficiency of RB (Qmax: 579.01 mg g(-1) within 40 min) and CR (Qmax: 233.24 mg g(-1) within 30 min) dyes from aqueous environment.
26002148	7	67	theme	Desorption	1182:1191	arg1	study					1193:1197	Desorption study	1182:1197	Desorption study	1182:1197	Desorption study predicts the excellent regenerative efficacy of nanocomposite.
26002148	3	68	theme	aqueous	760:766	arg1	environment					768:778	aqueous environment	760:778	aqueous environment	760:778	The composite demonstrates rapid and superior adsorption efficiency of RB (Qmax: 579.01 mg g(-1) within 40 min) and CR (Qmax: 233.24 mg g(-1) within 30 min) dyes from aqueous environment.
26002148	1	69	theme	synthesized	219:229	arg1	nanocomposite					290:302	sol-gel synthesized guar gum-graft-poly (acrylamide)/silica (g-GG/SiO2) hybrid nanocomposite	211:302	sol-gel synthesized guar gum-graft-poly (acrylamide)/silica (g-GG/SiO2) hybrid nanocomposite	211:302	The present study highlights the potentiality of sol-gel synthesized guar gum-graft-poly (acrylamide)/silica (g-GG/SiO2) hybrid nanocomposite toward the rapid removal of toxic reactive blue 4 (RB) and Congo red (CR) dyes from aqueous solution.
26002148	0	70	theme	dyes	156:159	arg1	removal					111:117	removal	111:117	removal of toxic reactive blue and Congo red dyes	111:159	Efficient and rapid adsorption characteristics of templating modified guar gum and silica nanocomposite toward removal of toxic reactive blue and Congo red dyes.
26002148	1	71	theme	dyes	378:381	arg1	removal					321:327	the rapid removal	311:327	the rapid removal of toxic reactive blue 4 (RB) and Congo red (CR) dyes from aqueous solution	311:403	The present study highlights the potentiality of sol-gel synthesized guar gum-graft-poly (acrylamide)/silica (g-GG/SiO2) hybrid nanocomposite toward the rapid removal of toxic reactive blue 4 (RB) and Congo red (CR) dyes from aqueous solution.
26002148	5	72	theme	adsorption	885:894	arg1	data					905:908	The adsorption kinetics data	881:908	The adsorption kinetics data	881:908	The adsorption kinetics data predicts that pseudo second-order (surface adsorption) and intraparticle diffusion take place simultaneously.
26002148	6	73	theme	adsorption	1024:1033	arg1	equilibrium					1035:1045	The adsorption equilibrium	1020:1045	The adsorption equilibrium	1020:1045	The adsorption equilibrium is in good agreement with the Langmuir isotherm, while the thermodynamics study confirms spontaneous nature of the adsorption process.
26002148	1	74	theme	guar	231:234	arg1	nanocomposite					290:302	sol-gel synthesized guar gum-graft-poly (acrylamide)/silica (g-GG/SiO2) hybrid nanocomposite	211:302	sol-gel synthesized guar gum-graft-poly (acrylamide)/silica (g-GG/SiO2) hybrid nanocomposite	211:302	The present study highlights the potentiality of sol-gel synthesized guar gum-graft-poly (acrylamide)/silica (g-GG/SiO2) hybrid nanocomposite toward the rapid removal of toxic reactive blue 4 (RB) and Congo red (CR) dyes from aqueous solution.
26002148	2	75	theme	functionalized	479:492	arg1	gum					499:501	the functionalized guar gum	475:501	the functionalized guar gum matrix	475:508	Various physicochemical characterizations support the feasibility of the functionalized guar gum matrix as efficient template for the formation of homogeneous nanoscale silica particles.
26002148	5	76	theme	kinetics	896:903	arg1	data					905:908	The adsorption kinetics data	881:908	The adsorption kinetics data	881:908	The adsorption kinetics data predicts that pseudo second-order (surface adsorption) and intraparticle diffusion take place simultaneously.
26002148	6	77	theme	Langmuir	1077:1084	arg1	isotherm					1086:1093	the Langmuir isotherm	1073:1093	the Langmuir isotherm	1073:1093	The adsorption equilibrium is in good agreement with the Langmuir isotherm, while the thermodynamics study confirms spontaneous nature of the adsorption process.
26002148	3	78	theme	mg	726:727	arg1	g					729:729	Qmax: 233.24 mg g(-1)	713:733	Qmax: 233.24 mg g(-1)	713:733	The composite demonstrates rapid and superior adsorption efficiency of RB (Qmax: 579.01 mg g(-1) within 40 min) and CR (Qmax: 233.24 mg g(-1) within 30 min) dyes from aqueous environment.
26002148	1	79	theme	gum-graft-poly	236:249	arg1	nanocomposite					290:302	sol-gel synthesized guar gum-graft-poly (acrylamide)/silica (g-GG/SiO2) hybrid nanocomposite	211:302	sol-gel synthesized guar gum-graft-poly (acrylamide)/silica (g-GG/SiO2) hybrid nanocomposite	211:302	The present study highlights the potentiality of sol-gel synthesized guar gum-graft-poly (acrylamide)/silica (g-GG/SiO2) hybrid nanocomposite toward the rapid removal of toxic reactive blue 4 (RB) and Congo red (CR) dyes from aqueous solution.
26002148	0	80	theme	guar	70:73	arg1	gum					75:77	modified guar gum	61:77	modified guar gum	61:77	Efficient and rapid adsorption characteristics of templating modified guar gum and silica nanocomposite toward removal of toxic reactive blue and Congo red dyes.
26002148	7	81	theme	excellent	1212:1220	arg1	efficacy					1235:1242	the excellent regenerative efficacy	1208:1242	the excellent regenerative efficacy of nanocomposite	1208:1259	Desorption study predicts the excellent regenerative efficacy of nanocomposite.
26002148	1	82	dep	red	369:371	arg1	CR					374:375	CR	374:375	CR	374:375	The present study highlights the potentiality of sol-gel synthesized guar gum-graft-poly (acrylamide)/silica (g-GG/SiO2) hybrid nanocomposite toward the rapid removal of toxic reactive blue 4 (RB) and Congo red (CR) dyes from aqueous solution.
24268233	0	0	theme	wound	81:85	arg1	dressings					87:95	potential wound dressings	71:95	potential wound dressings	71:95	Poly(vinyl alcohol)/cellulose nanowhiskers nanocomposite hydrogels for potential wound dressings.
24268233	6	1	theme	pore	853:856	arg1	morphology					858:867	the pore morphology	849:867	the pore morphology of the samples	849:882	It was found that the addition of CNW to the hydrogel allows controlling the pore morphology of the samples.
24268233	7	2	theme	water	1037:1041	arg1	vapor					1043:1047	water vapor	1037:1047	the water vapor transmission rate	1033:1065	On the other hand, the transparency of the samples was maintained, the thermal stability was increased, the mechanical properties were improved and the water vapor transmission rate was in the range of wound dressing applications after CNW incorporation inside the PVA hydrogel matrix.
24268233	1	3	theme	CNW	145:147	arg1	hydrogels					164:172	Polyvinyl alcohol (PVA)/cellulose nanowhisker (CNW) nanocomposite hydrogels	98:172	Polyvinyl alcohol (PVA)/cellulose nanowhisker (CNW) nanocomposite hydrogels to be used for wound dressing	98:202	Polyvinyl alcohol (PVA)/cellulose nanowhisker (CNW) nanocomposite hydrogels to be used for wound dressing were obtained by freezing-thawing technique and characterized by means of morphological, physical, thermal, mechanical, barrier and antimicrobial properties.
24268233	0	4	theme	potential	71:79	arg1	dressings					87:95	potential wound dressings	71:95	potential wound dressings	71:95	Poly(vinyl alcohol)/cellulose nanowhiskers nanocomposite hydrogels for potential wound dressings.
24268233	1	5	theme	barrier	324:330	arg1	properties					350:359	morphological, physical, thermal, mechanical, barrier and antimicrobial properties	278:359	properties	350:359	Polyvinyl alcohol (PVA)/cellulose nanowhisker (CNW) nanocomposite hydrogels to be used for wound dressing were obtained by freezing-thawing technique and characterized by means of morphological, physical, thermal, mechanical, barrier and antimicrobial properties.
24268233	7	6	theme	vapor	1043:1047	arg1	rate					1062:1065	the water vapor transmission rate	1033:1065	the water vapor transmission rate	1033:1065	On the other hand, the transparency of the samples was maintained, the thermal stability was increased, the mechanical properties were improved and the water vapor transmission rate was in the range of wound dressing applications after CNW incorporation inside the PVA hydrogel matrix.
24268233	7	7	theme	other	892:896	arg1	hand					898:901	the other hand	888:901	the other hand	888:901	On the other hand, the transparency of the samples was maintained, the thermal stability was increased, the mechanical properties were improved and the water vapor transmission rate was in the range of wound dressing applications after CNW incorporation inside the PVA hydrogel matrix.
24268233	7	8	theme	transmission	1049:1060	arg1	rate					1062:1065	the water vapor transmission rate	1033:1065	the water vapor transmission rate	1033:1065	On the other hand, the transparency of the samples was maintained, the thermal stability was increased, the mechanical properties were improved and the water vapor transmission rate was in the range of wound dressing applications after CNW incorporation inside the PVA hydrogel matrix.
24268233	5	9	theme	thermal	672:678	arg1	characterization					703:718	Morphological, thermal, chemical and physical characterization	657:718	Morphological, thermal, chemical and physical characterization of the PVA/CNW nanocomposite hydrogels	657:757	Morphological, thermal, chemical and physical characterization of the PVA/CNW nanocomposite hydrogels was carried out.
24268233	1	10	theme	antimicrobial	336:348	arg1	properties					350:359	morphological, physical, thermal, mechanical, barrier and antimicrobial properties	278:359	properties	350:359	Polyvinyl alcohol (PVA)/cellulose nanowhisker (CNW) nanocomposite hydrogels to be used for wound dressing were obtained by freezing-thawing technique and characterized by means of morphological, physical, thermal, mechanical, barrier and antimicrobial properties.
24268233	7	11	theme	hydrogel	1154:1161	arg1	matrix					1163:1168	the PVA hydrogel matrix	1146:1168	the PVA hydrogel matrix	1146:1168	On the other hand, the transparency of the samples was maintained, the thermal stability was increased, the mechanical properties were improved and the water vapor transmission rate was in the range of wound dressing applications after CNW incorporation inside the PVA hydrogel matrix.
24268233	7	12	theme	dressing	1093:1100	arg1	applications					1102:1113	wound dressing applications	1087:1113	wound dressing applications	1087:1113	On the other hand, the transparency of the samples was maintained, the thermal stability was increased, the mechanical properties were improved and the water vapor transmission rate was in the range of wound dressing applications after CNW incorporation inside the PVA hydrogel matrix.
24268233	1	13	theme	nanocomposite	150:162	arg1	hydrogels					164:172	Polyvinyl alcohol (PVA)/cellulose nanowhisker (CNW) nanocomposite hydrogels	98:172	Polyvinyl alcohol (PVA)/cellulose nanowhisker (CNW) nanocomposite hydrogels to be used for wound dressing	98:202	Polyvinyl alcohol (PVA)/cellulose nanowhisker (CNW) nanocomposite hydrogels to be used for wound dressing were obtained by freezing-thawing technique and characterized by means of morphological, physical, thermal, mechanical, barrier and antimicrobial properties.
24268233	5	14	theme	hydrogels	749:757	arg1	characterization					703:718	Morphological, thermal, chemical and physical characterization	657:718	Morphological, thermal, chemical and physical characterization of the PVA/CNW nanocomposite hydrogels	657:757	Morphological, thermal, chemical and physical characterization of the PVA/CNW nanocomposite hydrogels was carried out.
24268233	8	15	theme	good	1269:1272	arg1	barrier					1274:1280	a good barrier	1267:1280	a good barrier against different microorganisms	1267:1313	The evaluation of microbial penetration showed that the prepared hydrogels can be considered as a good barrier against different microorganisms.
24268233	8	15	theme	good	1269:1272	arg1	hydrogels					1236:1244	the prepared hydrogels	1223:1244	the prepared hydrogels	1223:1244	The evaluation of microbial penetration showed that the prepared hydrogels can be considered as a good barrier against different microorganisms.
24268233	2	16	theme	commercial	432:441	arg1	MCC					471:473	MCC	471:473	MCC	471:473	First, cellulose nanowhiskers were obtained by the acid hydrolysis of commercial crystalline microcellulose (MCC) and characterized by its size, shape, morphological, structural and thermal properties.
24268233	2	16	theme	commercial	432:441	arg1	microcellulose					455:468	commercial crystalline microcellulose	432:468	commercial crystalline microcellulose (MCC)	432:474	First, cellulose nanowhiskers were obtained by the acid hydrolysis of commercial crystalline microcellulose (MCC) and characterized by its size, shape, morphological, structural and thermal properties.
24268233	2	17	theme	crystalline	443:453	arg1	MCC					471:473	MCC	471:473	MCC	471:473	First, cellulose nanowhiskers were obtained by the acid hydrolysis of commercial crystalline microcellulose (MCC) and characterized by its size, shape, morphological, structural and thermal properties.
24268233	2	17	theme	crystalline	443:453	arg1	microcellulose					455:468	commercial crystalline microcellulose	432:468	commercial crystalline microcellulose (MCC)	432:474	First, cellulose nanowhiskers were obtained by the acid hydrolysis of commercial crystalline microcellulose (MCC) and characterized by its size, shape, morphological, structural and thermal properties.
24268233	7	18	theme	mechanical	993:1002	arg1	properties					1004:1013	the mechanical properties	989:1013	the mechanical properties	989:1013	On the other hand, the transparency of the samples was maintained, the thermal stability was increased, the mechanical properties were improved and the water vapor transmission rate was in the range of wound dressing applications after CNW incorporation inside the PVA hydrogel matrix.
24268233	8	19	theme	different	1290:1298	arg1	microorganisms					1300:1313	different microorganisms	1290:1313	different microorganisms	1290:1313	The evaluation of microbial penetration showed that the prepared hydrogels can be considered as a good barrier against different microorganisms.
24268233	9	20	used	used	1393:1396	arg2	materials					1363:1371	the PVA/CNW materials	1351:1371	the PVA/CNW materials	1351:1371	All obtained results indicate that the PVA/CNW materials are promising to be used as wound dressing.
24268233	9	20	used	used	1393:1396	arg2	dressing					1407:1414	wound dressing	1401:1414	wound dressing	1401:1414	All obtained results indicate that the PVA/CNW materials are promising to be used as wound dressing.
24268233	9	20	used	used	1393:1396	arg2	promising					1377:1385	promising	1377:1385	promising	1377:1385	All obtained results indicate that the PVA/CNW materials are promising to be used as wound dressing.
24268233	2	21	theme	cellulose	369:377	arg1	nanowhiskers					379:390	cellulose nanowhiskers	369:390	cellulose nanowhiskers	369:390	First, cellulose nanowhiskers were obtained by the acid hydrolysis of commercial crystalline microcellulose (MCC) and characterized by its size, shape, morphological, structural and thermal properties.
24268233	0	22	theme	Poly	0:3	arg1	nanowhiskers					30:41	Poly(vinyl alcohol)/cellulose nanowhiskers	0:41	Poly(vinyl alcohol)/cellulose nanowhiskers	0:41	Poly(vinyl alcohol)/cellulose nanowhiskers nanocomposite hydrogels for potential wound dressings.
24268233	1	23	theme	Polyvinyl	98:106	arg1	PVA					117:119	PVA	117:119	PVA	117:119	Polyvinyl alcohol (PVA)/cellulose nanowhisker (CNW) nanocomposite hydrogels to be used for wound dressing were obtained by freezing-thawing technique and characterized by means of morphological, physical, thermal, mechanical, barrier and antimicrobial properties.
24268233	1	23	theme	Polyvinyl	98:106	arg1	alcohol					108:114	Polyvinyl alcohol	98:114	Polyvinyl alcohol (PVA)/cellulose nanowhisker (CNW) nanocomposite hydrogels to be used for wound dressing	98:202	Polyvinyl alcohol (PVA)/cellulose nanowhisker (CNW) nanocomposite hydrogels to be used for wound dressing were obtained by freezing-thawing technique and characterized by means of morphological, physical, thermal, mechanical, barrier and antimicrobial properties.
24268233	2	24	theme	acid	413:416	arg1	hydrolysis					418:427	the acid hydrolysis	409:427	the acid hydrolysis of commercial crystalline microcellulose (MCC)	409:474	First, cellulose nanowhiskers were obtained by the acid hydrolysis of commercial crystalline microcellulose (MCC) and characterized by its size, shape, morphological, structural and thermal properties.
24268233	0	25	theme	vinyl	5:9	arg1	Poly					0:3	Poly	0:3	Poly(vinyl alcohol)/cellulose nanowhiskers	0:41	Poly(vinyl alcohol)/cellulose nanowhiskers nanocomposite hydrogels for potential wound dressings.
24268233	0	25	theme	vinyl	5:9	arg1	alcohol					11:17	vinyl alcohol	5:17	vinyl alcohol	5:17	Poly(vinyl alcohol)/cellulose nanowhiskers nanocomposite hydrogels for potential wound dressings.
24268233	1	26	theme	morphological	278:290	arg1	barrier					324:330	morphological, physical, thermal, mechanical, barrier and antimicrobial properties	278:359	barrier	324:330	Polyvinyl alcohol (PVA)/cellulose nanowhisker (CNW) nanocomposite hydrogels to be used for wound dressing were obtained by freezing-thawing technique and characterized by means of morphological, physical, thermal, mechanical, barrier and antimicrobial properties.
24268233	2	27	theme	structural	529:538	arg1	properties					552:561	morphological, structural and thermal properties	514:561	morphological, structural and thermal properties	514:561	First, cellulose nanowhiskers were obtained by the acid hydrolysis of commercial crystalline microcellulose (MCC) and characterized by its size, shape, morphological, structural and thermal properties.
24268233	2	27	theme	structural	529:538	arg1	size					501:504	its size	497:504	its size	497:504	First, cellulose nanowhiskers were obtained by the acid hydrolysis of commercial crystalline microcellulose (MCC) and characterized by its size, shape, morphological, structural and thermal properties.
24268233	6	28	theme	CNW	810:812	arg1	addition					798:805	the addition	794:805	the addition of CNW to the hydrogel	794:828	It was found that the addition of CNW to the hydrogel allows controlling the pore morphology of the samples.
24268233	1	29	theme	alcohol	108:114	arg1	hydrogels					164:172	Polyvinyl alcohol (PVA)/cellulose nanowhisker (CNW) nanocomposite hydrogels	98:172	Polyvinyl alcohol (PVA)/cellulose nanowhisker (CNW) nanocomposite hydrogels to be used for wound dressing	98:202	Polyvinyl alcohol (PVA)/cellulose nanowhisker (CNW) nanocomposite hydrogels to be used for wound dressing were obtained by freezing-thawing technique and characterized by means of morphological, physical, thermal, mechanical, barrier and antimicrobial properties.
24268233	0	30	theme	/cellulose	19:28	arg1	nanowhiskers					30:41	Poly(vinyl alcohol)/cellulose nanowhiskers	0:41	Poly(vinyl alcohol)/cellulose nanowhiskers	0:41	Poly(vinyl alcohol)/cellulose nanowhiskers nanocomposite hydrogels for potential wound dressings.
24268233	3	31	dep	contents	610:617	arg1	5					629:629	5	629:629	5	629:629	Then, PVA/CNW nanocomposites with several CNW contents (0, 1, 3, 5 and 7wt.
24268233	5	32	theme	Morphological	657:669	arg1	characterization					703:718	Morphological, thermal, chemical and physical characterization	657:718	Morphological, thermal, chemical and physical characterization of the PVA/CNW nanocomposite hydrogels	657:757	Morphological, thermal, chemical and physical characterization of the PVA/CNW nanocomposite hydrogels was carried out.
24268233	1	33	theme	wound	189:193	arg1	dressing					195:202	wound dressing	189:202	wound dressing	189:202	Polyvinyl alcohol (PVA)/cellulose nanowhisker (CNW) nanocomposite hydrogels to be used for wound dressing were obtained by freezing-thawing technique and characterized by means of morphological, physical, thermal, mechanical, barrier and antimicrobial properties.
24268233	2	34	theme	morphological	514:526	arg1	properties					552:561	morphological, structural and thermal properties	514:561	morphological, structural and thermal properties	514:561	First, cellulose nanowhiskers were obtained by the acid hydrolysis of commercial crystalline microcellulose (MCC) and characterized by its size, shape, morphological, structural and thermal properties.
24268233	2	34	theme	morphological	514:526	arg1	size					501:504	its size	497:504	its size	497:504	First, cellulose nanowhiskers were obtained by the acid hydrolysis of commercial crystalline microcellulose (MCC) and characterized by its size, shape, morphological, structural and thermal properties.
24268233	8	35	theme	penetration	1199:1209	arg1	evaluation					1175:1184	The evaluation	1171:1184	The evaluation of microbial penetration	1171:1209	The evaluation of microbial penetration showed that the prepared hydrogels can be considered as a good barrier against different microorganisms.
24268233	5	36	theme	chemical	681:688	arg1	characterization					703:718	Morphological, thermal, chemical and physical characterization	657:718	Morphological, thermal, chemical and physical characterization of the PVA/CNW nanocomposite hydrogels	657:757	Morphological, thermal, chemical and physical characterization of the PVA/CNW nanocomposite hydrogels was carried out.
24268233	5	37	theme	nanocomposite	735:747	arg1	hydrogels					749:757	the PVA/CNW nanocomposite hydrogels	723:757	the PVA/CNW nanocomposite hydrogels	723:757	Morphological, thermal, chemical and physical characterization of the PVA/CNW nanocomposite hydrogels was carried out.
24268233	6	38	theme	samples	876:882	arg1	morphology					858:867	the pore morphology	849:867	the pore morphology of the samples	849:882	It was found that the addition of CNW to the hydrogel allows controlling the pore morphology of the samples.
24268233	7	39	theme	thermal	956:962	arg1	stability					964:972	the thermal stability	952:972	the thermal stability	952:972	On the other hand, the transparency of the samples was maintained, the thermal stability was increased, the mechanical properties were improved and the water vapor transmission rate was in the range of wound dressing applications after CNW incorporation inside the PVA hydrogel matrix.
24268233	7	40	theme	PVA	1150:1152	arg1	matrix					1163:1168	the PVA hydrogel matrix	1146:1168	the PVA hydrogel matrix	1146:1168	On the other hand, the transparency of the samples was maintained, the thermal stability was increased, the mechanical properties were improved and the water vapor transmission rate was in the range of wound dressing applications after CNW incorporation inside the PVA hydrogel matrix.
24268233	9	41	theme	wound	1401:1405	arg1	materials					1363:1371	the PVA/CNW materials	1351:1371	the PVA/CNW materials	1351:1371	All obtained results indicate that the PVA/CNW materials are promising to be used as wound dressing.
24268233	9	41	theme	wound	1401:1405	arg1	dressing					1407:1414	wound dressing	1401:1414	wound dressing	1401:1414	All obtained results indicate that the PVA/CNW materials are promising to be used as wound dressing.
24268233	2	42	theme	microcellulose	455:468	arg1	hydrolysis					418:427	the acid hydrolysis	409:427	the acid hydrolysis of commercial crystalline microcellulose (MCC)	409:474	First, cellulose nanowhiskers were obtained by the acid hydrolysis of commercial crystalline microcellulose (MCC) and characterized by its size, shape, morphological, structural and thermal properties.
24268233	7	43	theme	applications	1102:1113	arg1	range					1078:1082	the range	1074:1082	the range of wound dressing applications	1074:1113	On the other hand, the transparency of the samples was maintained, the thermal stability was increased, the mechanical properties were improved and the water vapor transmission rate was in the range of wound dressing applications after CNW incorporation inside the PVA hydrogel matrix.
24268233	1	44	dep	hydrogels	164:172	arg1	used					180:183	used	180:183	to be used for wound dressing	174:202	Polyvinyl alcohol (PVA)/cellulose nanowhisker (CNW) nanocomposite hydrogels to be used for wound dressing were obtained by freezing-thawing technique and characterized by means of morphological, physical, thermal, mechanical, barrier and antimicrobial properties.
24268233	7	45	theme	CNW	1121:1123	arg1	incorporation					1125:1137	CNW incorporation	1121:1137	CNW incorporation inside the PVA hydrogel matrix	1121:1168	On the other hand, the transparency of the samples was maintained, the thermal stability was increased, the mechanical properties were improved and the water vapor transmission rate was in the range of wound dressing applications after CNW incorporation inside the PVA hydrogel matrix.
24268233	7	46	theme	samples	928:934	arg1	transparency					908:919	the transparency	904:919	the transparency of the samples	904:934	On the other hand, the transparency of the samples was maintained, the thermal stability was increased, the mechanical properties were improved and the water vapor transmission rate was in the range of wound dressing applications after CNW incorporation inside the PVA hydrogel matrix.
24268233	8	47	theme	prepared	1227:1234	arg1	barrier					1274:1280	a good barrier	1267:1280	a good barrier against different microorganisms	1267:1313	The evaluation of microbial penetration showed that the prepared hydrogels can be considered as a good barrier against different microorganisms.
24268233	8	47	theme	prepared	1227:1234	arg1	hydrogels					1236:1244	the prepared hydrogels	1223:1244	the prepared hydrogels	1223:1244	The evaluation of microbial penetration showed that the prepared hydrogels can be considered as a good barrier against different microorganisms.
24268233	2	48	theme	thermal	544:550	arg1	properties					552:561	morphological, structural and thermal properties	514:561	morphological, structural and thermal properties	514:561	First, cellulose nanowhiskers were obtained by the acid hydrolysis of commercial crystalline microcellulose (MCC) and characterized by its size, shape, morphological, structural and thermal properties.
24268233	2	48	theme	thermal	544:550	arg1	size					501:504	its size	497:504	its size	497:504	First, cellulose nanowhiskers were obtained by the acid hydrolysis of commercial crystalline microcellulose (MCC) and characterized by its size, shape, morphological, structural and thermal properties.
24268233	1	49	theme	/cellulose	121:130	arg1	hydrogels					164:172	Polyvinyl alcohol (PVA)/cellulose nanowhisker (CNW) nanocomposite hydrogels	98:172	Polyvinyl alcohol (PVA)/cellulose nanowhisker (CNW) nanocomposite hydrogels to be used for wound dressing	98:202	Polyvinyl alcohol (PVA)/cellulose nanowhisker (CNW) nanocomposite hydrogels to be used for wound dressing were obtained by freezing-thawing technique and characterized by means of morphological, physical, thermal, mechanical, barrier and antimicrobial properties.
24268233	9	50	theme	obtained	1320:1327	arg1	results					1329:1335	All obtained results	1316:1335	All obtained results	1316:1335	All obtained results indicate that the PVA/CNW materials are promising to be used as wound dressing.
24268233	8	51	theme	microbial	1189:1197	arg1	penetration					1199:1209	microbial penetration	1189:1209	microbial penetration	1189:1209	The evaluation of microbial penetration showed that the prepared hydrogels can be considered as a good barrier against different microorganisms.
24268233	3	52	theme	several	598:604	arg1	contents					610:617	several CNW contents	598:617	several CNW contents (0, 1, 3, 5	598:629	Then, PVA/CNW nanocomposites with several CNW contents (0, 1, 3, 5 and 7wt.
24268233	1	53	theme	nanowhisker	132:142	arg1	hydrogels					164:172	Polyvinyl alcohol (PVA)/cellulose nanowhisker (CNW) nanocomposite hydrogels	98:172	Polyvinyl alcohol (PVA)/cellulose nanowhisker (CNW) nanocomposite hydrogels to be used for wound dressing	98:202	Polyvinyl alcohol (PVA)/cellulose nanowhisker (CNW) nanocomposite hydrogels to be used for wound dressing were obtained by freezing-thawing technique and characterized by means of morphological, physical, thermal, mechanical, barrier and antimicrobial properties.
24268233	5	54	theme	physical	694:701	arg1	characterization					703:718	Morphological, thermal, chemical and physical characterization	657:718	Morphological, thermal, chemical and physical characterization of the PVA/CNW nanocomposite hydrogels	657:757	Morphological, thermal, chemical and physical characterization of the PVA/CNW nanocomposite hydrogels was carried out.
24268233	1	55	dep	morphological	278:290	arg1	mechanical					312:321	mechanical	312:321	mechanical	312:321	Polyvinyl alcohol (PVA)/cellulose nanowhisker (CNW) nanocomposite hydrogels to be used for wound dressing were obtained by freezing-thawing technique and characterized by means of morphological, physical, thermal, mechanical, barrier and antimicrobial properties.
24268233	1	55	dep	morphological	278:290	arg1	thermal					303:309	thermal	303:309	thermal	303:309	Polyvinyl alcohol (PVA)/cellulose nanowhisker (CNW) nanocomposite hydrogels to be used for wound dressing were obtained by freezing-thawing technique and characterized by means of morphological, physical, thermal, mechanical, barrier and antimicrobial properties.
24268233	1	55	dep	morphological	278:290	arg1	physical					293:300	physical	293:300	physical	293:300	Polyvinyl alcohol (PVA)/cellulose nanowhisker (CNW) nanocomposite hydrogels to be used for wound dressing were obtained by freezing-thawing technique and characterized by means of morphological, physical, thermal, mechanical, barrier and antimicrobial properties.
24268233	3	56	theme	CNW	606:608	arg1	contents					610:617	several CNW contents	598:617	several CNW contents (0, 1, 3, 5	598:629	Then, PVA/CNW nanocomposites with several CNW contents (0, 1, 3, 5 and 7wt.
24268233	7	57	theme	wound	1087:1091	arg1	applications					1102:1113	wound dressing applications	1087:1113	wound dressing applications	1087:1113	On the other hand, the transparency of the samples was maintained, the thermal stability was increased, the mechanical properties were improved and the water vapor transmission rate was in the range of wound dressing applications after CNW incorporation inside the PVA hydrogel matrix.
24268233	1	58	theme	freezing-thawing	221:236	arg1	technique					238:246	freezing-thawing technique	221:246	freezing-thawing technique	221:246	Polyvinyl alcohol (PVA)/cellulose nanowhisker (CNW) nanocomposite hydrogels to be used for wound dressing were obtained by freezing-thawing technique and characterized by means of morphological, physical, thermal, mechanical, barrier and antimicrobial properties.
27179144	5	0	theme	scaffolds	1101:1109	arg1	surface					1083:1089	the surface	1079:1089	the surface of soaked scaffolds in simulated body fluid	1079:1133	The in vitro bioactivity of the composites was confirmed by the formation of a calcium phosphate rich layer on the surface of soaked scaffolds in simulated body fluid.
27179144	1	1	theme	scanning	321:328	arg1	microscopy					339:348	scanning electron microscopy	321:348	scanning electron microscopy (SEM and FE-SEM)	321:365	The composite scaffolds of the chitosan and multiphasic calcium phosphate (HW) short fibers were prepared by freeze drying and characterized by X-ray diffractometry (XRD), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM and FE-SEM).
27179144	4	2	theme	3.87MPa	945:951	arg1	strength					910:917	a mean strength	903:917	a mean strength	903:917	The strongest composite scaffolds in this study with a chitosan: HW fibers weight ratio of 1:1 showed a mean porosity of 69% and a mean strength and modulus of 420kPa and 3.87MPa, respectively.
27179144	4	2	theme	3.87MPa	945:951	arg1	420kPa					934:939	420kPa	934:939	420kPa	934:939	The strongest composite scaffolds in this study with a chitosan: HW fibers weight ratio of 1:1 showed a mean porosity of 69% and a mean strength and modulus of 420kPa and 3.87MPa, respectively.
27179144	4	2	theme	3.87MPa	945:951	arg1	3.87MPa					945:951	3.87MPa	945:951	3.87MPa	945:951	The strongest composite scaffolds in this study with a chitosan: HW fibers weight ratio of 1:1 showed a mean porosity of 69% and a mean strength and modulus of 420kPa and 3.87MPa, respectively.
27179144	4	2	theme	3.87MPa	945:951	arg1	%					897:897	69%	895:897	69%	895:897	The strongest composite scaffolds in this study with a chitosan: HW fibers weight ratio of 1:1 showed a mean porosity of 69% and a mean strength and modulus of 420kPa and 3.87MPa, respectively.
27179144	4	2	theme	3.87MPa	945:951	arg1	modulus					923:929	modulus	923:929	modulus	923:929	The strongest composite scaffolds in this study with a chitosan: HW fibers weight ratio of 1:1 showed a mean porosity of 69% and a mean strength and modulus of 420kPa and 3.87MPa, respectively.
27179144	3	3	from	increase	641:648	arg1	size					658:661	pore size	653:661	pore size	653:661	The incorporation of HW fibers consisting three phases of hydroxyapatite (HA), beta-tricalcium phosphate (β-TCP) and calcium pyrophosphate (CPP) into the chitosan matrices was associated with an increase in pore size, density and compressive strength and modulus, and a decrease in porosity and swelling ratio of the scaffolds.
27179144	3	3	from	increase	641:648	arg1	ratio					750:754	porosity and swelling ratio	728:754	porosity and swelling ratio of the scaffolds	728:771	The incorporation of HW fibers consisting three phases of hydroxyapatite (HA), beta-tricalcium phosphate (β-TCP) and calcium pyrophosphate (CPP) into the chitosan matrices was associated with an increase in pore size, density and compressive strength and modulus, and a decrease in porosity and swelling ratio of the scaffolds.
27179144	3	3	from	increase	641:648	arg1	density					664:670	density	664:670	density	664:670	The incorporation of HW fibers consisting three phases of hydroxyapatite (HA), beta-tricalcium phosphate (β-TCP) and calcium pyrophosphate (CPP) into the chitosan matrices was associated with an increase in pore size, density and compressive strength and modulus, and a decrease in porosity and swelling ratio of the scaffolds.
27179144	3	3	from	increase	641:648	arg1	strength					688:695	compressive strength	676:695	compressive strength	676:695	The incorporation of HW fibers consisting three phases of hydroxyapatite (HA), beta-tricalcium phosphate (β-TCP) and calcium pyrophosphate (CPP) into the chitosan matrices was associated with an increase in pore size, density and compressive strength and modulus, and a decrease in porosity and swelling ratio of the scaffolds.
27179144	1	4	theme	electron	330:337	arg1	microscopy					339:348	scanning electron microscopy	321:348	scanning electron microscopy (SEM and FE-SEM)	321:365	The composite scaffolds of the chitosan and multiphasic calcium phosphate (HW) short fibers were prepared by freeze drying and characterized by X-ray diffractometry (XRD), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM and FE-SEM).
27179144	3	5	from	decrease	716:723	arg1	ratio					750:754	porosity and swelling ratio	728:754	porosity and swelling ratio of the scaffolds	728:771	The incorporation of HW fibers consisting three phases of hydroxyapatite (HA), beta-tricalcium phosphate (β-TCP) and calcium pyrophosphate (CPP) into the chitosan matrices was associated with an increase in pore size, density and compressive strength and modulus, and a decrease in porosity and swelling ratio of the scaffolds.
27179144	3	5	from	decrease	716:723	arg1	size					658:661	pore size	653:661	pore size	653:661	The incorporation of HW fibers consisting three phases of hydroxyapatite (HA), beta-tricalcium phosphate (β-TCP) and calcium pyrophosphate (CPP) into the chitosan matrices was associated with an increase in pore size, density and compressive strength and modulus, and a decrease in porosity and swelling ratio of the scaffolds.
27179144	3	5	from	decrease	716:723	arg1	density					664:670	density	664:670	density	664:670	The incorporation of HW fibers consisting three phases of hydroxyapatite (HA), beta-tricalcium phosphate (β-TCP) and calcium pyrophosphate (CPP) into the chitosan matrices was associated with an increase in pore size, density and compressive strength and modulus, and a decrease in porosity and swelling ratio of the scaffolds.
27179144	3	5	from	decrease	716:723	arg1	strength					688:695	compressive strength	676:695	compressive strength	676:695	The incorporation of HW fibers consisting three phases of hydroxyapatite (HA), beta-tricalcium phosphate (β-TCP) and calcium pyrophosphate (CPP) into the chitosan matrices was associated with an increase in pore size, density and compressive strength and modulus, and a decrease in porosity and swelling ratio of the scaffolds.
27179144	1	6	dep	Fourier	270:276	arg1	transform					278:286	transform	278:286	transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM and FE-SEM)	278:365	The composite scaffolds of the chitosan and multiphasic calcium phosphate (HW) short fibers were prepared by freeze drying and characterized by X-ray diffractometry (XRD), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM and FE-SEM).
27179144	6	7	theme	initial	1157:1163	arg1	work					1165:1168	this initial work	1152:1168	this initial work	1152:1168	The findings of this initial work indicate that the chitosan-multiphasic calcium phosphate short fibers may be a suitable material for bone scaffolding.
27179144	5	8	theme	layer	1070:1074	arg1	formation					1032:1040	the formation	1028:1040	the formation of a calcium phosphate rich layer on the surface of soaked scaffolds in simulated body fluid	1028:1133	The in vitro bioactivity of the composites was confirmed by the formation of a calcium phosphate rich layer on the surface of soaked scaffolds in simulated body fluid.
27179144	4	9	theme	%	897:897	arg1	porosity					883:890	a mean porosity	876:890	a mean porosity of 69% and a mean strength and modulus of 420kPa and 3.87MPa, respectively	876:965	The strongest composite scaffolds in this study with a chitosan: HW fibers weight ratio of 1:1 showed a mean porosity of 69% and a mean strength and modulus of 420kPa and 3.87MPa, respectively.
27179144	6	10	theme	suitable	1249:1256	arg1	material					1258:1265	a suitable material	1247:1265	a suitable material for bone scaffolding	1247:1286	The findings of this initial work indicate that the chitosan-multiphasic calcium phosphate short fibers may be a suitable material for bone scaffolding.
27179144	6	10	theme	suitable	1249:1256	arg1	phosphate					1217:1225	the chitosan-multiphasic calcium phosphate short fibers	1184:1238	the chitosan-multiphasic calcium phosphate short fibers	1184:1238	The findings of this initial work indicate that the chitosan-multiphasic calcium phosphate short fibers may be a suitable material for bone scaffolding.
27179144	3	11	theme	compressive	676:686	arg1	strength					688:695	compressive strength	676:695	compressive strength	676:695	The incorporation of HW fibers consisting three phases of hydroxyapatite (HA), beta-tricalcium phosphate (β-TCP) and calcium pyrophosphate (CPP) into the chitosan matrices was associated with an increase in pore size, density and compressive strength and modulus, and a decrease in porosity and swelling ratio of the scaffolds.
27179144	6	12	theme	short	1227:1231	arg1	fibers					1233:1238	short fibers	1227:1238	the chitosan-multiphasic calcium phosphate short fibers	1184:1238	The findings of this initial work indicate that the chitosan-multiphasic calcium phosphate short fibers may be a suitable material for bone scaffolding.
27179144	3	13	theme	HW	467:468	arg1	fibers					470:475	HW fibers	467:475	HW fibers consisting three phases of hydroxyapatite (HA), beta-tricalcium phosphate (β-TCP) and calcium pyrophosphate (CPP)	467:589	The incorporation of HW fibers consisting three phases of hydroxyapatite (HA), beta-tricalcium phosphate (β-TCP) and calcium pyrophosphate (CPP) into the chitosan matrices was associated with an increase in pore size, density and compressive strength and modulus, and a decrease in porosity and swelling ratio of the scaffolds.
27179144	4	14	from	scaffolds	798:806	arg1	study					816:820	this study	811:820	this study	811:820	The strongest composite scaffolds in this study with a chitosan: HW fibers weight ratio of 1:1 showed a mean porosity of 69% and a mean strength and modulus of 420kPa and 3.87MPa, respectively.
27179144	4	15	theme	composite	788:796	arg1	scaffolds					798:806	The strongest composite scaffolds	774:806	The strongest composite scaffolds in this study with a chitosan: HW fibers weight ratio of 1:1 showed a mean porosity of 69% and a mean strength and modulus of 420kPa and 3.87MPa, respectively.	774:966	The strongest composite scaffolds in this study with a chitosan: HW fibers weight ratio of 1:1 showed a mean porosity of 69% and a mean strength and modulus of 420kPa and 3.87MPa, respectively.
27179144	4	16	with	scaffolds	798:806	arg1	chitosan					829:836	a chitosan	827:836	a chitosan	827:836	The strongest composite scaffolds in this study with a chitosan: HW fibers weight ratio of 1:1 showed a mean porosity of 69% and a mean strength and modulus of 420kPa and 3.87MPa, respectively.
27179144	6	17	theme	chitosan-multiphasic	1188:1207	arg1	material					1258:1265	a suitable material	1247:1265	a suitable material for bone scaffolding	1247:1286	The findings of this initial work indicate that the chitosan-multiphasic calcium phosphate short fibers may be a suitable material for bone scaffolding.
27179144	6	17	theme	chitosan-multiphasic	1188:1207	arg1	phosphate					1217:1225	the chitosan-multiphasic calcium phosphate short fibers	1184:1238	the chitosan-multiphasic calcium phosphate short fibers	1184:1238	The findings of this initial work indicate that the chitosan-multiphasic calcium phosphate short fibers may be a suitable material for bone scaffolding.
27179144	2	18	theme	mechanical	372:381	arg1	properties					383:392	The mechanical properties	368:392	The mechanical properties of the scaffolds	368:409	The mechanical properties of the scaffolds were assessed by compression test.
27179144	3	19	theme	scaffolds	763:771	arg1	ratio					750:754	porosity and swelling ratio	728:754	porosity and swelling ratio of the scaffolds	728:771	The incorporation of HW fibers consisting three phases of hydroxyapatite (HA), beta-tricalcium phosphate (β-TCP) and calcium pyrophosphate (CPP) into the chitosan matrices was associated with an increase in pore size, density and compressive strength and modulus, and a decrease in porosity and swelling ratio of the scaffolds.
27179144	5	20	theme	composites	1000:1009	arg1	bioactivity					981:991	The in vitro bioactivity	968:991	The in vitro bioactivity of the composites	968:1009	The in vitro bioactivity of the composites was confirmed by the formation of a calcium phosphate rich layer on the surface of soaked scaffolds in simulated body fluid.
27179144	4	21	theme	strongest	778:786	arg1	scaffolds					798:806	The strongest composite scaffolds	774:806	The strongest composite scaffolds in this study with a chitosan: HW fibers weight ratio of 1:1 showed a mean porosity of 69% and a mean strength and modulus of 420kPa and 3.87MPa, respectively.	774:966	The strongest composite scaffolds in this study with a chitosan: HW fibers weight ratio of 1:1 showed a mean porosity of 69% and a mean strength and modulus of 420kPa and 3.87MPa, respectively.
27179144	1	22	dep	chitosan	129:136	arg1	fibers					183:188	short fibers	177:188	short fibers	177:188	The composite scaffolds of the chitosan and multiphasic calcium phosphate (HW) short fibers were prepared by freeze drying and characterized by X-ray diffractometry (XRD), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM and FE-SEM).
27179144	1	22	dep	chitosan	129:136	arg1	the					125:127	the	125:127	the	125:127	The composite scaffolds of the chitosan and multiphasic calcium phosphate (HW) short fibers were prepared by freeze drying and characterized by X-ray diffractometry (XRD), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM and FE-SEM).
27179144	1	23	theme	short	177:181	arg1	fibers					183:188	short fibers	177:188	short fibers	177:188	The composite scaffolds of the chitosan and multiphasic calcium phosphate (HW) short fibers were prepared by freeze drying and characterized by X-ray diffractometry (XRD), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM and FE-SEM).
27179144	2	24	theme	compression	428:438	arg1	test					440:443	compression test	428:443	compression test	428:443	The mechanical properties of the scaffolds were assessed by compression test.
27179144	3	25	theme	hydroxyapatite	504:517	arg1	phases					494:499	three phases	488:499	three phases of hydroxyapatite (HA)	488:522	The incorporation of HW fibers consisting three phases of hydroxyapatite (HA), beta-tricalcium phosphate (β-TCP) and calcium pyrophosphate (CPP) into the chitosan matrices was associated with an increase in pore size, density and compressive strength and modulus, and a decrease in porosity and swelling ratio of the scaffolds.
27179144	3	25	theme	hydroxyapatite	504:517	arg1	pyrophosphate					571:583	calcium pyrophosphate	563:583	calcium pyrophosphate (CPP)	563:589	The incorporation of HW fibers consisting three phases of hydroxyapatite (HA), beta-tricalcium phosphate (β-TCP) and calcium pyrophosphate (CPP) into the chitosan matrices was associated with an increase in pore size, density and compressive strength and modulus, and a decrease in porosity and swelling ratio of the scaffolds.
27179144	3	25	theme	hydroxyapatite	504:517	arg1	phosphate					541:549	beta-tricalcium phosphate	525:549	beta-tricalcium phosphate (β-TCP)	525:557	The incorporation of HW fibers consisting three phases of hydroxyapatite (HA), beta-tricalcium phosphate (β-TCP) and calcium pyrophosphate (CPP) into the chitosan matrices was associated with an increase in pore size, density and compressive strength and modulus, and a decrease in porosity and swelling ratio of the scaffolds.
27179144	5	26	dep	in	972:973	arg1	vitro					975:979	vitro	975:979	vitro	975:979	The in vitro bioactivity of the composites was confirmed by the formation of a calcium phosphate rich layer on the surface of soaked scaffolds in simulated body fluid.
27179144	3	27	theme	pore	653:656	arg1	size					658:661	pore size	653:661	pore size	653:661	The incorporation of HW fibers consisting three phases of hydroxyapatite (HA), beta-tricalcium phosphate (β-TCP) and calcium pyrophosphate (CPP) into the chitosan matrices was associated with an increase in pore size, density and compressive strength and modulus, and a decrease in porosity and swelling ratio of the scaffolds.
27179144	4	28	dep	scaffolds	798:806	arg1	showed					869:874	showed	869:874	showed a mean porosity of 69% and a mean strength and modulus of 420kPa and 3.87MPa, respectively	869:965	The strongest composite scaffolds in this study with a chitosan: HW fibers weight ratio of 1:1 showed a mean porosity of 69% and a mean strength and modulus of 420kPa and 3.87MPa, respectively.
27179144	5	29	theme	rich	1065:1068	arg1	layer					1070:1074	a calcium phosphate rich layer	1045:1074	a calcium phosphate rich layer	1045:1074	The in vitro bioactivity of the composites was confirmed by the formation of a calcium phosphate rich layer on the surface of soaked scaffolds in simulated body fluid.
27179144	1	30	theme	composite	102:110	arg1	scaffolds					112:120	The composite scaffolds	98:120	The composite scaffolds of the chitosan and multiphasic calcium phosphate (HW) short fibers	98:188	The composite scaffolds of the chitosan and multiphasic calcium phosphate (HW) short fibers were prepared by freeze drying and characterized by X-ray diffractometry (XRD), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM and FE-SEM).
27179144	5	31	from	scaffolds	1101:1109	arg1	fluid					1129:1133	simulated body fluid	1114:1133	simulated body fluid	1114:1133	The in vitro bioactivity of the composites was confirmed by the formation of a calcium phosphate rich layer on the surface of soaked scaffolds in simulated body fluid.
27179144	6	32	theme	work	1165:1168	arg1	findings					1140:1147	The findings	1136:1147	The findings of this initial work	1136:1168	The findings of this initial work indicate that the chitosan-multiphasic calcium phosphate short fibers may be a suitable material for bone scaffolding.
27179144	0	33	theme	chitosan	30:37	arg1	Reinforcement					0:12	Reinforcement	0:12	Reinforcement of freeze-dried chitosan	0:37	Reinforcement of freeze-dried chitosan scaffolds with multiphasic calcium phosphate short fibers.
27179144	1	34	dep	microscopy	339:348	arg1	SEM					351:353	SEM	351:353	SEM	351:353	The composite scaffolds of the chitosan and multiphasic calcium phosphate (HW) short fibers were prepared by freeze drying and characterized by X-ray diffractometry (XRD), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM and FE-SEM).
27179144	1	34	dep	microscopy	339:348	arg1	FE-SEM					359:364	FE-SEM	359:364	FE-SEM	359:364	The composite scaffolds of the chitosan and multiphasic calcium phosphate (HW) short fibers were prepared by freeze drying and characterized by X-ray diffractometry (XRD), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM and FE-SEM).
27179144	3	35	theme	beta-tricalcium	525:539	arg1	phosphate					541:549	beta-tricalcium phosphate	525:549	beta-tricalcium phosphate (β-TCP)	525:557	The incorporation of HW fibers consisting three phases of hydroxyapatite (HA), beta-tricalcium phosphate (β-TCP) and calcium pyrophosphate (CPP) into the chitosan matrices was associated with an increase in pore size, density and compressive strength and modulus, and a decrease in porosity and swelling ratio of the scaffolds.
27179144	3	35	theme	beta-tricalcium	525:539	arg1	phases					494:499	three phases	488:499	three phases of hydroxyapatite (HA)	488:522	The incorporation of HW fibers consisting three phases of hydroxyapatite (HA), beta-tricalcium phosphate (β-TCP) and calcium pyrophosphate (CPP) into the chitosan matrices was associated with an increase in pore size, density and compressive strength and modulus, and a decrease in porosity and swelling ratio of the scaffolds.
27179144	3	35	theme	beta-tricalcium	525:539	arg1	β-TCP					552:556	β-TCP	552:556	β-TCP	552:556	The incorporation of HW fibers consisting three phases of hydroxyapatite (HA), beta-tricalcium phosphate (β-TCP) and calcium pyrophosphate (CPP) into the chitosan matrices was associated with an increase in pore size, density and compressive strength and modulus, and a decrease in porosity and swelling ratio of the scaffolds.
27179144	2	36	theme	scaffolds	401:409	arg1	properties					383:392	The mechanical properties	368:392	The mechanical properties of the scaffolds	368:409	The mechanical properties of the scaffolds were assessed by compression test.
27179144	0	37	theme	freeze-dried	17:28	arg1	chitosan					30:37	freeze-dried chitosan	17:37	freeze-dried chitosan	17:37	Reinforcement of freeze-dried chitosan scaffolds with multiphasic calcium phosphate short fibers.
27179144	4	38	theme	weight	849:854	arg1	ratio					856:860	HW fibers weight ratio	839:860	HW fibers weight ratio of 1:1	839:867	The strongest composite scaffolds in this study with a chitosan: HW fibers weight ratio of 1:1 showed a mean porosity of 69% and a mean strength and modulus of 420kPa and 3.87MPa, respectively.
27179144	4	39	theme	mean	905:908	arg1	strength					910:917	a mean strength	903:917	a mean strength	903:917	The strongest composite scaffolds in this study with a chitosan: HW fibers weight ratio of 1:1 showed a mean porosity of 69% and a mean strength and modulus of 420kPa and 3.87MPa, respectively.
27179144	4	40	theme	1:1	865:867	arg1	ratio					856:860	HW fibers weight ratio	839:860	HW fibers weight ratio of 1:1	839:867	The strongest composite scaffolds in this study with a chitosan: HW fibers weight ratio of 1:1 showed a mean porosity of 69% and a mean strength and modulus of 420kPa and 3.87MPa, respectively.
27179144	5	41	theme	in	972:973	arg1	bioactivity					981:991	The in vitro bioactivity	968:991	The in vitro bioactivity of the composites	968:1009	The in vitro bioactivity of the composites was confirmed by the formation of a calcium phosphate rich layer on the surface of soaked scaffolds in simulated body fluid.
27179144	3	42	theme	calcium	563:569	arg1	CPP					586:588	CPP	586:588	CPP	586:588	The incorporation of HW fibers consisting three phases of hydroxyapatite (HA), beta-tricalcium phosphate (β-TCP) and calcium pyrophosphate (CPP) into the chitosan matrices was associated with an increase in pore size, density and compressive strength and modulus, and a decrease in porosity and swelling ratio of the scaffolds.
27179144	3	42	theme	calcium	563:569	arg1	pyrophosphate					571:583	calcium pyrophosphate	563:583	calcium pyrophosphate (CPP)	563:589	The incorporation of HW fibers consisting three phases of hydroxyapatite (HA), beta-tricalcium phosphate (β-TCP) and calcium pyrophosphate (CPP) into the chitosan matrices was associated with an increase in pore size, density and compressive strength and modulus, and a decrease in porosity and swelling ratio of the scaffolds.
27179144	3	42	theme	calcium	563:569	arg1	phases					494:499	three phases	488:499	three phases of hydroxyapatite (HA)	488:522	The incorporation of HW fibers consisting three phases of hydroxyapatite (HA), beta-tricalcium phosphate (β-TCP) and calcium pyrophosphate (CPP) into the chitosan matrices was associated with an increase in pore size, density and compressive strength and modulus, and a decrease in porosity and swelling ratio of the scaffolds.
27179144	1	43	theme	freeze	207:212	arg1	drying					214:219	freeze drying	207:219	freeze drying	207:219	The composite scaffolds of the chitosan and multiphasic calcium phosphate (HW) short fibers were prepared by freeze drying and characterized by X-ray diffractometry (XRD), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM and FE-SEM).
27179144	5	44	from	formation	1032:1040	arg1	surface					1083:1089	the surface	1079:1089	the surface of soaked scaffolds in simulated body fluid	1079:1133	The in vitro bioactivity of the composites was confirmed by the formation of a calcium phosphate rich layer on the surface of soaked scaffolds in simulated body fluid.
27179144	4	45	theme	strength	910:917	arg1	porosity					883:890	a mean porosity	876:890	a mean porosity of 69% and a mean strength and modulus of 420kPa and 3.87MPa, respectively	876:965	The strongest composite scaffolds in this study with a chitosan: HW fibers weight ratio of 1:1 showed a mean porosity of 69% and a mean strength and modulus of 420kPa and 3.87MPa, respectively.
27179144	6	46	dep	phosphate	1217:1225	arg1	fibers					1233:1238	short fibers	1227:1238	the chitosan-multiphasic calcium phosphate short fibers	1184:1238	The findings of this initial work indicate that the chitosan-multiphasic calcium phosphate short fibers may be a suitable material for bone scaffolding.
27179144	5	47	from	surface	1083:1089	arg1	fluid					1129:1133	simulated body fluid	1114:1133	simulated body fluid	1114:1133	The in vitro bioactivity of the composites was confirmed by the formation of a calcium phosphate rich layer on the surface of soaked scaffolds in simulated body fluid.
27179144	4	48	theme	mean	878:881	arg1	porosity					883:890	a mean porosity	876:890	a mean porosity of 69% and a mean strength and modulus of 420kPa and 3.87MPa, respectively	876:965	The strongest composite scaffolds in this study with a chitosan: HW fibers weight ratio of 1:1 showed a mean porosity of 69% and a mean strength and modulus of 420kPa and 3.87MPa, respectively.
27179144	0	49	theme	calcium	66:72	arg1	fibers					90:95	multiphasic calcium phosphate short fibers	54:95	multiphasic calcium phosphate short fibers	54:95	Reinforcement of freeze-dried chitosan scaffolds with multiphasic calcium phosphate short fibers.
27179144	3	50	theme	porosity	728:735	arg1	ratio					750:754	porosity and swelling ratio	728:754	porosity and swelling ratio of the scaffolds	728:771	The incorporation of HW fibers consisting three phases of hydroxyapatite (HA), beta-tricalcium phosphate (β-TCP) and calcium pyrophosphate (CPP) into the chitosan matrices was associated with an increase in pore size, density and compressive strength and modulus, and a decrease in porosity and swelling ratio of the scaffolds.
27179144	6	51	theme	calcium	1209:1215	arg1	material					1258:1265	a suitable material	1247:1265	a suitable material for bone scaffolding	1247:1286	The findings of this initial work indicate that the chitosan-multiphasic calcium phosphate short fibers may be a suitable material for bone scaffolding.
27179144	6	51	theme	calcium	1209:1215	arg1	phosphate					1217:1225	the chitosan-multiphasic calcium phosphate short fibers	1184:1238	the chitosan-multiphasic calcium phosphate short fibers	1184:1238	The findings of this initial work indicate that the chitosan-multiphasic calcium phosphate short fibers may be a suitable material for bone scaffolding.
27179144	3	52	theme	chitosan	600:607	arg1	matrices					609:616	the chitosan matrices	596:616	the chitosan matrices	596:616	The incorporation of HW fibers consisting three phases of hydroxyapatite (HA), beta-tricalcium phosphate (β-TCP) and calcium pyrophosphate (CPP) into the chitosan matrices was associated with an increase in pore size, density and compressive strength and modulus, and a decrease in porosity and swelling ratio of the scaffolds.
27179144	3	53	theme	fibers	470:475	arg1	incorporation					450:462	The incorporation	446:462	The incorporation of HW fibers consisting three phases of hydroxyapatite (HA), beta-tricalcium phosphate (β-TCP) and calcium pyrophosphate (CPP) into the chitosan matrices	446:616	The incorporation of HW fibers consisting three phases of hydroxyapatite (HA), beta-tricalcium phosphate (β-TCP) and calcium pyrophosphate (CPP) into the chitosan matrices was associated with an increase in pore size, density and compressive strength and modulus, and a decrease in porosity and swelling ratio of the scaffolds.
27179144	0	54	theme	multiphasic	54:64	arg1	fibers					90:95	multiphasic calcium phosphate short fibers	54:95	multiphasic calcium phosphate short fibers	54:95	Reinforcement of freeze-dried chitosan scaffolds with multiphasic calcium phosphate short fibers.
27179144	5	55	from	fluid	1129:1133	arg1	surface					1083:1089	the surface	1079:1089	the surface of soaked scaffolds in simulated body fluid	1079:1133	The in vitro bioactivity of the composites was confirmed by the formation of a calcium phosphate rich layer on the surface of soaked scaffolds in simulated body fluid.
27179144	1	56	theme	chitosan	129:136	arg1	scaffolds					112:120	The composite scaffolds	98:120	The composite scaffolds of the chitosan and multiphasic calcium phosphate (HW) short fibers	98:188	The composite scaffolds of the chitosan and multiphasic calcium phosphate (HW) short fibers were prepared by freeze drying and characterized by X-ray diffractometry (XRD), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM and FE-SEM).
27179144	6	57	theme	bone	1271:1274	arg1	scaffolding					1276:1286	bone scaffolding	1271:1286	bone scaffolding	1271:1286	The findings of this initial work indicate that the chitosan-multiphasic calcium phosphate short fibers may be a suitable material for bone scaffolding.
27179144	4	58	theme	fibers	842:847	arg1	ratio					856:860	HW fibers weight ratio	839:860	HW fibers weight ratio of 1:1	839:867	The strongest composite scaffolds in this study with a chitosan: HW fibers weight ratio of 1:1 showed a mean porosity of 69% and a mean strength and modulus of 420kPa and 3.87MPa, respectively.
27179144	4	59	theme	420kPa	934:939	arg1	strength					910:917	a mean strength	903:917	a mean strength	903:917	The strongest composite scaffolds in this study with a chitosan: HW fibers weight ratio of 1:1 showed a mean porosity of 69% and a mean strength and modulus of 420kPa and 3.87MPa, respectively.
27179144	4	59	theme	420kPa	934:939	arg1	420kPa					934:939	420kPa	934:939	420kPa	934:939	The strongest composite scaffolds in this study with a chitosan: HW fibers weight ratio of 1:1 showed a mean porosity of 69% and a mean strength and modulus of 420kPa and 3.87MPa, respectively.
27179144	4	59	theme	420kPa	934:939	arg1	3.87MPa					945:951	3.87MPa	945:951	3.87MPa	945:951	The strongest composite scaffolds in this study with a chitosan: HW fibers weight ratio of 1:1 showed a mean porosity of 69% and a mean strength and modulus of 420kPa and 3.87MPa, respectively.
27179144	4	59	theme	420kPa	934:939	arg1	%					897:897	69%	895:897	69%	895:897	The strongest composite scaffolds in this study with a chitosan: HW fibers weight ratio of 1:1 showed a mean porosity of 69% and a mean strength and modulus of 420kPa and 3.87MPa, respectively.
27179144	4	59	theme	420kPa	934:939	arg1	modulus					923:929	modulus	923:929	modulus	923:929	The strongest composite scaffolds in this study with a chitosan: HW fibers weight ratio of 1:1 showed a mean porosity of 69% and a mean strength and modulus of 420kPa and 3.87MPa, respectively.
27179144	5	60	theme	soaked	1094:1099	arg1	scaffolds					1101:1109	soaked scaffolds	1094:1109	soaked scaffolds in simulated body fluid	1094:1133	The in vitro bioactivity of the composites was confirmed by the formation of a calcium phosphate rich layer on the surface of soaked scaffolds in simulated body fluid.
27179144	1	61	theme	multiphasic	142:152	arg1	phosphate					162:170	multiphasic calcium phosphate	142:170	multiphasic calcium phosphate (HW)	142:175	The composite scaffolds of the chitosan and multiphasic calcium phosphate (HW) short fibers were prepared by freeze drying and characterized by X-ray diffractometry (XRD), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM and FE-SEM).
27179144	1	61	theme	multiphasic	142:152	arg1	HW					173:174	HW	173:174	HW	173:174	The composite scaffolds of the chitosan and multiphasic calcium phosphate (HW) short fibers were prepared by freeze drying and characterized by X-ray diffractometry (XRD), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM and FE-SEM).
27179144	5	62	theme	simulated	1114:1122	arg1	fluid					1129:1133	simulated body fluid	1114:1133	simulated body fluid	1114:1133	The in vitro bioactivity of the composites was confirmed by the formation of a calcium phosphate rich layer on the surface of soaked scaffolds in simulated body fluid.
27179144	0	63	theme	short	84:88	arg1	fibers					90:95	multiphasic calcium phosphate short fibers	54:95	multiphasic calcium phosphate short fibers	54:95	Reinforcement of freeze-dried chitosan scaffolds with multiphasic calcium phosphate short fibers.
27179144	1	64	theme	calcium	154:160	arg1	phosphate					162:170	multiphasic calcium phosphate	142:170	multiphasic calcium phosphate (HW)	142:175	The composite scaffolds of the chitosan and multiphasic calcium phosphate (HW) short fibers were prepared by freeze drying and characterized by X-ray diffractometry (XRD), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM and FE-SEM).
27179144	1	64	theme	calcium	154:160	arg1	HW					173:174	HW	173:174	HW	173:174	The composite scaffolds of the chitosan and multiphasic calcium phosphate (HW) short fibers were prepared by freeze drying and characterized by X-ray diffractometry (XRD), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM and FE-SEM).
27179144	5	65	theme	body	1124:1127	arg1	fluid					1129:1133	simulated body fluid	1114:1133	simulated body fluid	1114:1133	The in vitro bioactivity of the composites was confirmed by the formation of a calcium phosphate rich layer on the surface of soaked scaffolds in simulated body fluid.
27179144	0	66	theme	phosphate	74:82	arg1	fibers					90:95	multiphasic calcium phosphate short fibers	54:95	multiphasic calcium phosphate short fibers	54:95	Reinforcement of freeze-dried chitosan scaffolds with multiphasic calcium phosphate short fibers.
27179144	1	67	dep	transform	278:286	arg1	infrared					288:295	infrared	288:295	transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM and FE-SEM)	278:365	The composite scaffolds of the chitosan and multiphasic calcium phosphate (HW) short fibers were prepared by freeze drying and characterized by X-ray diffractometry (XRD), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM and FE-SEM).
27179144	3	68	theme	swelling	741:748	arg1	ratio					750:754	porosity and swelling ratio	728:754	porosity and swelling ratio of the scaffolds	728:771	The incorporation of HW fibers consisting three phases of hydroxyapatite (HA), beta-tricalcium phosphate (β-TCP) and calcium pyrophosphate (CPP) into the chitosan matrices was associated with an increase in pore size, density and compressive strength and modulus, and a decrease in porosity and swelling ratio of the scaffolds.
27179144	1	69	theme	phosphate	162:170	arg1	scaffolds					112:120	The composite scaffolds	98:120	The composite scaffolds of the chitosan and multiphasic calcium phosphate (HW) short fibers	98:188	The composite scaffolds of the chitosan and multiphasic calcium phosphate (HW) short fibers were prepared by freeze drying and characterized by X-ray diffractometry (XRD), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM and FE-SEM).
27179144	1	70	theme	X-ray	242:246	arg1	XRD					264:266	XRD	264:266	XRD	264:266	The composite scaffolds of the chitosan and multiphasic calcium phosphate (HW) short fibers were prepared by freeze drying and characterized by X-ray diffractometry (XRD), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM and FE-SEM).
27179144	1	70	theme	X-ray	242:246	arg1	Fourier					270:276	Fourier	270:276	Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM and FE-SEM)	270:365	The composite scaffolds of the chitosan and multiphasic calcium phosphate (HW) short fibers were prepared by freeze drying and characterized by X-ray diffractometry (XRD), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM and FE-SEM).
27179144	1	70	theme	X-ray	242:246	arg1	diffractometry					248:261	X-ray diffractometry	242:261	X-ray diffractometry (XRD)	242:267	The composite scaffolds of the chitosan and multiphasic calcium phosphate (HW) short fibers were prepared by freeze drying and characterized by X-ray diffractometry (XRD), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM and FE-SEM).
24370423	6	0	theme	different	859:867	arg1	centuries					869:877	different centuries	859:877	different centuries	859:877	FINDINGS Gellan hydrogel has been used to clean paper samples belonging to different centuries (from XVI to XIX) and therefore, characterized by a different story in terms of degradation condition and paper composition.
24370423	6	1	from	story	941:945	arg1	terms					950:954	terms	950:954	terms of degradation condition and paper composition	950:1001	FINDINGS Gellan hydrogel has been used to clean paper samples belonging to different centuries (from XVI to XIX) and therefore, characterized by a different story in terms of degradation condition and paper composition.
24370423	6	2	theme	condition	971:979	arg1	terms					950:954	terms	950:954	terms of degradation condition and paper composition	950:1001	FINDINGS Gellan hydrogel has been used to clean paper samples belonging to different centuries (from XVI to XIX) and therefore, characterized by a different story in terms of degradation condition and paper composition.
24370423	3	3	theme	water	475:479	arg1	inks					491:494	water sensitive inks	475:494	water sensitive inks	475:494	Nevertheless, washing treatment usually involves a substantial impact on the original morphological structure of paper and can sometimes be dangerous for water sensitive inks and pigments.
24370423	4	4	theme	hydrogel	539:546	arg1	use					526:528	The use	522:528	The use of rigid hydrogel of Gellan gum	522:560	EXPERIMENTS The use of rigid hydrogel of Gellan gum as an alternative paper cleaning treatment is developed.
24370423	3	5	from	impact	384:389	arg1	structure					421:429	the original morphological structure	394:429	the original morphological structure of paper	394:438	Nevertheless, washing treatment usually involves a substantial impact on the original morphological structure of paper and can sometimes be dangerous for water sensitive inks and pigments.
24370423	2	6	theme	cellulose	298:306	arg1	degradation					308:318	cellulose degradation	298:318	cellulose degradation	298:318	It is used to improve the optical qualities of a graphic work and remove dust and by-products resulting from cellulose degradation.
24370423	2	7	theme	graphic	238:244	arg1	work					246:249	a graphic work	236:249	a graphic work	236:249	It is used to improve the optical qualities of a graphic work and remove dust and by-products resulting from cellulose degradation.
24370423	2	8	theme	work	246:249	arg1	qualities					223:231	the optical qualities	211:231	the optical qualities of a graphic work	211:249	It is used to improve the optical qualities of a graphic work and remove dust and by-products resulting from cellulose degradation.
24370423	4	9	theme	gum	558:560	arg1	hydrogel					539:546	rigid hydrogel	533:546	rigid hydrogel of Gellan gum	533:560	EXPERIMENTS The use of rigid hydrogel of Gellan gum as an alternative paper cleaning treatment is developed.
24370423	0	10	theme	detailed	64:71	arg1	study					73:77	a detailed study	62:77	a detailed study	62:77	Gellan hydrogel as a powerful tool in paper cleaning process: a detailed study.
24370423	7	11	theme	liquid	1049:1054	arg1	chromatography					1056:1069	high-performance liquid chromatography	1032:1069	high-performance liquid chromatography	1032:1069	Several techniques, such as high-performance liquid chromatography, Fourier transform infrared spectroscopy, scanning electron microscopy and pH measurements, has been employed to assess the effectiveness and safety of the proposed cleaning method.
24370423	4	12	theme	Gellan	551:556	arg1	gum					558:560	Gellan gum	551:560	Gellan gum	551:560	EXPERIMENTS The use of rigid hydrogel of Gellan gum as an alternative paper cleaning treatment is developed.
24370423	5	13	theme	water	694:698	arg1	use					687:689	the use	683:689	the use of water	683:698	The application of a rigid hydrogel minimizes damages caused by the use of water, and therefore is much more respectful for the original integrity of ancient paper.
24370423	6	14	theme	paper	832:836	arg1	samples					838:844	paper samples	832:844	paper samples	832:844	FINDINGS Gellan hydrogel has been used to clean paper samples belonging to different centuries (from XVI to XIX) and therefore, characterized by a different story in terms of degradation condition and paper composition.
24370423	7	15	theme	scanning	1113:1120	arg1	microscopy					1131:1140	scanning electron microscopy	1113:1140	scanning electron microscopy	1113:1140	Several techniques, such as high-performance liquid chromatography, Fourier transform infrared spectroscopy, scanning electron microscopy and pH measurements, has been employed to assess the effectiveness and safety of the proposed cleaning method.
24370423	4	16	theme	cleaning	586:593	arg1	treatment					595:603	an alternative paper cleaning treatment	565:603	an alternative paper cleaning treatment	565:603	EXPERIMENTS The use of rigid hydrogel of Gellan gum as an alternative paper cleaning treatment is developed.
24370423	0	17	dep	study	73:77	arg1	hydrogel					7:14	Gellan hydrogel	0:14	Gellan hydrogel as a powerful tool in paper cleaning process	0:59	Gellan hydrogel as a powerful tool in paper cleaning process: a detailed study.
24370423	6	18	theme	composition	991:1001	arg1	terms					950:954	terms	950:954	terms of degradation condition and paper composition	950:1001	FINDINGS Gellan hydrogel has been used to clean paper samples belonging to different centuries (from XVI to XIX) and therefore, characterized by a different story in terms of degradation condition and paper composition.
24370423	3	19	theme	original	398:405	arg1	structure					421:429	the original morphological structure	394:429	the original morphological structure of paper	394:438	Nevertheless, washing treatment usually involves a substantial impact on the original morphological structure of paper and can sometimes be dangerous for water sensitive inks and pigments.
24370423	7	20	theme	pH	1146:1147	arg1	measurements					1149:1160	pH measurements	1146:1160	pH measurements	1146:1160	Several techniques, such as high-performance liquid chromatography, Fourier transform infrared spectroscopy, scanning electron microscopy and pH measurements, has been employed to assess the effectiveness and safety of the proposed cleaning method.
24370423	0	21	from	tool	30:33	arg1	process					53:59	paper cleaning process	38:59	paper cleaning process	38:59	Gellan hydrogel as a powerful tool in paper cleaning process: a detailed study.
24370423	7	22	dep	transform	1080:1088	arg1	infrared					1090:1097	infrared	1090:1097	transform infrared spectroscopy	1080:1110	Several techniques, such as high-performance liquid chromatography, Fourier transform infrared spectroscopy, scanning electron microscopy and pH measurements, has been employed to assess the effectiveness and safety of the proposed cleaning method.
24370423	1	23	theme	most	136:139	arg1	steps					150:154	the most critical steps	132:154	the most critical steps	132:154	HYPOTHESIS Wet cleaning of ancient papers is one of the most critical steps during a conservation treatment.
24370423	5	24	theme	original	747:754	arg1	integrity					756:764	the original integrity	743:764	the original integrity of ancient paper	743:781	The application of a rigid hydrogel minimizes damages caused by the use of water, and therefore is much more respectful for the original integrity of ancient paper.
24370423	7	25	theme	cleaning	1236:1243	arg1	method					1245:1250	the proposed cleaning method	1223:1250	the proposed cleaning method	1223:1250	Several techniques, such as high-performance liquid chromatography, Fourier transform infrared spectroscopy, scanning electron microscopy and pH measurements, has been employed to assess the effectiveness and safety of the proposed cleaning method.
24370423	1	26	theme	critical	141:148	arg1	steps					150:154	the most critical steps	132:154	the most critical steps	132:154	HYPOTHESIS Wet cleaning of ancient papers is one of the most critical steps during a conservation treatment.
24370423	0	27	theme	Gellan	0:5	arg1	hydrogel					7:14	Gellan hydrogel	0:14	Gellan hydrogel as a powerful tool in paper cleaning process	0:59	Gellan hydrogel as a powerful tool in paper cleaning process: a detailed study.
24370423	7	28	dep	Fourier	1072:1078	arg1	transform					1080:1088	transform	1080:1088	transform infrared spectroscopy	1080:1110	Several techniques, such as high-performance liquid chromatography, Fourier transform infrared spectroscopy, scanning electron microscopy and pH measurements, has been employed to assess the effectiveness and safety of the proposed cleaning method.
24370423	1	29	theme	steps	150:154	arg1	steps					150:154	the most critical steps	132:154	the most critical steps	132:154	HYPOTHESIS Wet cleaning of ancient papers is one of the most critical steps during a conservation treatment.
24370423	1	29	theme	steps	150:154	arg1	one					125:127	one	125:127	one	125:127	HYPOTHESIS Wet cleaning of ancient papers is one of the most critical steps during a conservation treatment.
24370423	7	30	dep	effectiveness	1195:1207	arg1	the					1191:1193	the	1191:1193	the	1191:1193	Several techniques, such as high-performance liquid chromatography, Fourier transform infrared spectroscopy, scanning electron microscopy and pH measurements, has been employed to assess the effectiveness and safety of the proposed cleaning method.
24370423	3	31	theme	paper	434:438	arg1	structure					421:429	the original morphological structure	394:429	the original morphological structure of paper	394:438	Nevertheless, washing treatment usually involves a substantial impact on the original morphological structure of paper and can sometimes be dangerous for water sensitive inks and pigments.
24370423	5	32	theme	ancient	769:775	arg1	paper					777:781	ancient paper	769:781	ancient paper	769:781	The application of a rigid hydrogel minimizes damages caused by the use of water, and therefore is much more respectful for the original integrity of ancient paper.
24370423	4	33	theme	rigid	533:537	arg1	hydrogel					539:546	rigid hydrogel	533:546	rigid hydrogel of Gellan gum	533:560	EXPERIMENTS The use of rigid hydrogel of Gellan gum as an alternative paper cleaning treatment is developed.
24370423	7	34	theme	Several	1004:1010	arg1	measurements					1149:1160	pH measurements	1146:1160	pH measurements	1146:1160	Several techniques, such as high-performance liquid chromatography, Fourier transform infrared spectroscopy, scanning electron microscopy and pH measurements, has been employed to assess the effectiveness and safety of the proposed cleaning method.
24370423	7	34	theme	Several	1004:1010	arg1	techniques					1012:1021	Several techniques	1004:1021	Several techniques	1004:1021	Several techniques, such as high-performance liquid chromatography, Fourier transform infrared spectroscopy, scanning electron microscopy and pH measurements, has been employed to assess the effectiveness and safety of the proposed cleaning method.
24370423	7	34	theme	Several	1004:1010	arg1	chromatography					1056:1069	high-performance liquid chromatography	1032:1069	high-performance liquid chromatography	1032:1069	Several techniques, such as high-performance liquid chromatography, Fourier transform infrared spectroscopy, scanning electron microscopy and pH measurements, has been employed to assess the effectiveness and safety of the proposed cleaning method.
24370423	7	34	theme	Several	1004:1010	arg1	Fourier					1072:1078	Fourier	1072:1078	Fourier transform infrared spectroscopy	1072:1110	Several techniques, such as high-performance liquid chromatography, Fourier transform infrared spectroscopy, scanning electron microscopy and pH measurements, has been employed to assess the effectiveness and safety of the proposed cleaning method.
24370423	7	34	theme	Several	1004:1010	arg1	microscopy					1131:1140	scanning electron microscopy	1113:1140	scanning electron microscopy	1113:1140	Several techniques, such as high-performance liquid chromatography, Fourier transform infrared spectroscopy, scanning electron microscopy and pH measurements, has been employed to assess the effectiveness and safety of the proposed cleaning method.
24370423	1	35	theme	HYPOTHESIS	80:89	arg1	cleaning					95:102	HYPOTHESIS Wet cleaning	80:102	HYPOTHESIS Wet cleaning of ancient papers	80:120	HYPOTHESIS Wet cleaning of ancient papers is one of the most critical steps during a conservation treatment.
24370423	3	36	theme	sensitive	481:489	arg1	inks					491:494	water sensitive inks	475:494	water sensitive inks	475:494	Nevertheless, washing treatment usually involves a substantial impact on the original morphological structure of paper and can sometimes be dangerous for water sensitive inks and pigments.
24370423	3	37	theme	substantial	372:382	arg1	impact					384:389	a substantial impact	370:389	a substantial impact on the original morphological structure of paper	370:438	Nevertheless, washing treatment usually involves a substantial impact on the original morphological structure of paper and can sometimes be dangerous for water sensitive inks and pigments.
24370423	6	38	theme	degradation	959:969	arg1	condition					971:979	degradation condition	959:979	degradation condition	959:979	FINDINGS Gellan hydrogel has been used to clean paper samples belonging to different centuries (from XVI to XIX) and therefore, characterized by a different story in terms of degradation condition and paper composition.
24370423	2	39	used	used	195:198	arg2	It					189:190	It	189:190	It	189:190	It is used to improve the optical qualities of a graphic work and remove dust and by-products resulting from cellulose degradation.
24370423	1	40	theme	conservation	165:176	arg1	treatment					178:186	a conservation treatment	163:186	a conservation treatment	163:186	HYPOTHESIS Wet cleaning of ancient papers is one of the most critical steps during a conservation treatment.
24370423	0	41	theme	powerful	21:28	arg1	tool					30:33	a powerful tool	19:33	a powerful tool in paper cleaning process	19:59	Gellan hydrogel as a powerful tool in paper cleaning process: a detailed study.
24370423	6	42	dep	belonging	846:854	arg1	XIX					892:894	XIX	892:894	XIX	892:894	FINDINGS Gellan hydrogel has been used to clean paper samples belonging to different centuries (from XVI to XIX) and therefore, characterized by a different story in terms of degradation condition and paper composition.
24370423	6	43	dep	samples	838:844	arg1	belonging					846:854	belonging	846:854	belonging to different centuries (from XVI to XIX)	846:895	FINDINGS Gellan hydrogel has been used to clean paper samples belonging to different centuries (from XVI to XIX) and therefore, characterized by a different story in terms of degradation condition and paper composition.
24370423	7	44	theme	method	1245:1250	arg1	effectiveness					1195:1207	effectiveness	1195:1207	effectiveness	1195:1207	Several techniques, such as high-performance liquid chromatography, Fourier transform infrared spectroscopy, scanning electron microscopy and pH measurements, has been employed to assess the effectiveness and safety of the proposed cleaning method.
24370423	7	44	theme	method	1245:1250	arg1	safety					1213:1218	safety	1213:1218	safety	1213:1218	Several techniques, such as high-performance liquid chromatography, Fourier transform infrared spectroscopy, scanning electron microscopy and pH measurements, has been employed to assess the effectiveness and safety of the proposed cleaning method.
24370423	0	45	theme	paper	38:42	arg1	process					53:59	paper cleaning process	38:59	paper cleaning process	38:59	Gellan hydrogel as a powerful tool in paper cleaning process: a detailed study.
24370423	1	46	theme	Wet	91:93	arg1	cleaning					95:102	HYPOTHESIS Wet cleaning	80:102	HYPOTHESIS Wet cleaning of ancient papers	80:120	HYPOTHESIS Wet cleaning of ancient papers is one of the most critical steps during a conservation treatment.
24370423	6	47	used	used	818:821	arg2	hydrogel					800:807	FINDINGS Gellan hydrogel	784:807	FINDINGS Gellan hydrogel	784:807	FINDINGS Gellan hydrogel has been used to clean paper samples belonging to different centuries (from XVI to XIX) and therefore, characterized by a different story in terms of degradation condition and paper composition.
24370423	5	48	theme	rigid	640:644	arg1	hydrogel					646:653	a rigid hydrogel	638:653	a rigid hydrogel	638:653	The application of a rigid hydrogel minimizes damages caused by the use of water, and therefore is much more respectful for the original integrity of ancient paper.
24370423	6	49	theme	Gellan	793:798	arg1	hydrogel					800:807	FINDINGS Gellan hydrogel	784:807	FINDINGS Gellan hydrogel	784:807	FINDINGS Gellan hydrogel has been used to clean paper samples belonging to different centuries (from XVI to XIX) and therefore, characterized by a different story in terms of degradation condition and paper composition.
24370423	6	50	dep	XIX	892:894	arg1	XVI					885:887	XVI	885:887	XVI	885:887	FINDINGS Gellan hydrogel has been used to clean paper samples belonging to different centuries (from XVI to XIX) and therefore, characterized by a different story in terms of degradation condition and paper composition.
24370423	7	51	theme	proposed	1227:1234	arg1	method					1245:1250	the proposed cleaning method	1223:1250	the proposed cleaning method	1223:1250	Several techniques, such as high-performance liquid chromatography, Fourier transform infrared spectroscopy, scanning electron microscopy and pH measurements, has been employed to assess the effectiveness and safety of the proposed cleaning method.
24370423	5	52	theme	hydrogel	646:653	arg1	application					623:633	The application	619:633	The application of a rigid hydrogel	619:653	The application of a rigid hydrogel minimizes damages caused by the use of water, and therefore is much more respectful for the original integrity of ancient paper.
24370423	5	52	theme	hydrogel	646:653	arg1	respectful					728:737	respectful	728:737	respectful	728:737	The application of a rigid hydrogel minimizes damages caused by the use of water, and therefore is much more respectful for the original integrity of ancient paper.
24370423	6	53	theme	FINDINGS	784:791	arg1	hydrogel					800:807	FINDINGS Gellan hydrogel	784:807	FINDINGS Gellan hydrogel	784:807	FINDINGS Gellan hydrogel has been used to clean paper samples belonging to different centuries (from XVI to XIX) and therefore, characterized by a different story in terms of degradation condition and paper composition.
24370423	2	54	theme	optical	215:221	arg1	qualities					223:231	the optical qualities	211:231	the optical qualities of a graphic work	211:249	It is used to improve the optical qualities of a graphic work and remove dust and by-products resulting from cellulose degradation.
24370423	6	55	theme	paper	985:989	arg1	composition					991:1001	paper composition	985:1001	paper composition	985:1001	FINDINGS Gellan hydrogel has been used to clean paper samples belonging to different centuries (from XVI to XIX) and therefore, characterized by a different story in terms of degradation condition and paper composition.
24370423	7	56	theme	high-performance	1032:1047	arg1	chromatography					1056:1069	high-performance liquid chromatography	1032:1069	high-performance liquid chromatography	1032:1069	Several techniques, such as high-performance liquid chromatography, Fourier transform infrared spectroscopy, scanning electron microscopy and pH measurements, has been employed to assess the effectiveness and safety of the proposed cleaning method.
24370423	1	57	theme	ancient	107:113	arg1	papers					115:120	ancient papers	107:120	ancient papers	107:120	HYPOTHESIS Wet cleaning of ancient papers is one of the most critical steps during a conservation treatment.
24370423	5	58	theme	paper	777:781	arg1	integrity					756:764	the original integrity	743:764	the original integrity of ancient paper	743:781	The application of a rigid hydrogel minimizes damages caused by the use of water, and therefore is much more respectful for the original integrity of ancient paper.
24370423	1	59	theme	papers	115:120	arg1	cleaning					95:102	HYPOTHESIS Wet cleaning	80:102	HYPOTHESIS Wet cleaning of ancient papers	80:120	HYPOTHESIS Wet cleaning of ancient papers is one of the most critical steps during a conservation treatment.
24370423	3	60	theme	morphological	407:419	arg1	structure					421:429	the original morphological structure	394:429	the original morphological structure of paper	394:438	Nevertheless, washing treatment usually involves a substantial impact on the original morphological structure of paper and can sometimes be dangerous for water sensitive inks and pigments.
24370423	4	61	theme	paper	580:584	arg1	treatment					595:603	an alternative paper cleaning treatment	565:603	an alternative paper cleaning treatment	565:603	EXPERIMENTS The use of rigid hydrogel of Gellan gum as an alternative paper cleaning treatment is developed.
24370423	0	62	theme	cleaning	44:51	arg1	process					53:59	paper cleaning process	38:59	paper cleaning process	38:59	Gellan hydrogel as a powerful tool in paper cleaning process: a detailed study.
24370423	6	63	theme	different	931:939	arg1	story					941:945	a different story	929:945	a different story in terms of degradation condition and paper composition	929:1001	FINDINGS Gellan hydrogel has been used to clean paper samples belonging to different centuries (from XVI to XIX) and therefore, characterized by a different story in terms of degradation condition and paper composition.
24370423	7	64	theme	electron	1122:1129	arg1	microscopy					1131:1140	scanning electron microscopy	1113:1140	scanning electron microscopy	1113:1140	Several techniques, such as high-performance liquid chromatography, Fourier transform infrared spectroscopy, scanning electron microscopy and pH measurements, has been employed to assess the effectiveness and safety of the proposed cleaning method.
24370423	4	65	theme	alternative	568:578	arg1	treatment					595:603	an alternative paper cleaning treatment	565:603	an alternative paper cleaning treatment	565:603	EXPERIMENTS The use of rigid hydrogel of Gellan gum as an alternative paper cleaning treatment is developed.
28318250	0	0	theme	Coffee	95:100	arg1	Grounds					102:108	Spent Coffee Grounds	89:108	Spent Coffee Grounds	89:108	Chemical Characterization of Potentially Prebiotic Oligosaccharides in Brewed Coffee and Spent Coffee Grounds.
28318250	0	1	theme	Spent	89:93	arg1	Grounds					102:108	Spent Coffee Grounds	89:108	Spent Coffee Grounds	89:108	Chemical Characterization of Potentially Prebiotic Oligosaccharides in Brewed Coffee and Spent Coffee Grounds.
28318250	1	2	attach	present	166:172	arg1	plants					204:209	some plants	199:209	some plants	199:209	Oligosaccharides are indigestible carbohydrates widely present in mammalian milk and in some plants.
28318250	1	2	attach	present	166:172	arg2	Oligosaccharides					111:126	Oligosaccharides	111:126	Oligosaccharides	111:126	Oligosaccharides are indigestible carbohydrates widely present in mammalian milk and in some plants.
28318250	1	2	attach	present	166:172	arg1	milk					187:190	mammalian milk	177:190	mammalian milk	177:190	Oligosaccharides are indigestible carbohydrates widely present in mammalian milk and in some plants.
28318250	1	2	attach	present	166:172	arg2	carbohydrates					145:157	indigestible carbohydrates	132:157	indigestible carbohydrates widely present in mammalian milk and in some plants	132:209	Oligosaccharides are indigestible carbohydrates widely present in mammalian milk and in some plants.
28318250	1	3	from	milk	187:190	arg1	present					166:172	present	166:172	present	166:172	Oligosaccharides are indigestible carbohydrates widely present in mammalian milk and in some plants.
28318250	3	4	from	composition	408:418	arg1	beans					443:447	dark roasted coffee beans	423:447	dark roasted coffee beans	423:447	We investigated the oligosaccharides and their monomeric composition in dark roasted coffee beans, brewed coffee, and spent coffee grounds.
28318250	3	4	from	composition	408:418	arg1	grounds					482:488	spent coffee grounds	469:488	spent coffee grounds	469:488	We investigated the oligosaccharides and their monomeric composition in dark roasted coffee beans, brewed coffee, and spent coffee grounds.
28318250	3	4	from	composition	408:418	arg1	coffee					457:462	brewed coffee	450:462	brewed coffee	450:462	We investigated the oligosaccharides and their monomeric composition in dark roasted coffee beans, brewed coffee, and spent coffee grounds.
28318250	3	5	theme	monomeric	398:406	arg1	composition					408:418	their monomeric composition	392:418	their monomeric composition	392:418	We investigated the oligosaccharides and their monomeric composition in dark roasted coffee beans, brewed coffee, and spent coffee grounds.
28318250	4	6	dep	15	558:559	arg1	to					555:556	to	555:556	to	555:556	Oligosaccharides with a degree of polymerization ranging from 3 to 15, and their constituent monosaccharides, were characterized and quantified.
28318250	6	7	theme	coffee	895:900	arg1	samples					902:908	these coffee samples	889:908	these coffee samples	889:908	The diversity of oligosaccharides composition found in these coffee samples suggests that they could have selective prebiotic activity toward specific bacterial strains able to deconstruct the glycosidic bonds and utilize them as a carbon source.
28318250	5	8	theme	xylose	812:817	arg1	mixture					781:787	a heterogeneous mixture	765:787	a heterogeneous mixture of glucose, arabinose, xylose, and rhamnose	765:831	The oligosaccharides identified were mainly hexoses (potentially galacto-oligosaccharides and manno-oligosaccharides) containing a heterogeneous mixture of glucose, arabinose, xylose, and rhamnose.
28318250	0	9	theme	Chemical	0:7	arg1	Characterization					9:24	Chemical Characterization	0:24	Chemical Characterization of Potentially Prebiotic Oligosaccharides in Brewed Coffee and Spent Coffee Grounds.	0:109	Chemical Characterization of Potentially Prebiotic Oligosaccharides in Brewed Coffee and Spent Coffee Grounds.
28318250	3	10	from	oligosaccharides	371:386	arg1	beans					443:447	dark roasted coffee beans	423:447	dark roasted coffee beans	423:447	We investigated the oligosaccharides and their monomeric composition in dark roasted coffee beans, brewed coffee, and spent coffee grounds.
28318250	3	10	from	oligosaccharides	371:386	arg1	grounds					482:488	spent coffee grounds	469:488	spent coffee grounds	469:488	We investigated the oligosaccharides and their monomeric composition in dark roasted coffee beans, brewed coffee, and spent coffee grounds.
28318250	3	10	from	oligosaccharides	371:386	arg1	coffee					457:462	brewed coffee	450:462	brewed coffee	450:462	We investigated the oligosaccharides and their monomeric composition in dark roasted coffee beans, brewed coffee, and spent coffee grounds.
28318250	6	11	contain	have	935:938	arg1	they					924:927	they	924:927	they	924:927	The diversity of oligosaccharides composition found in these coffee samples suggests that they could have selective prebiotic activity toward specific bacterial strains able to deconstruct the glycosidic bonds and utilize them as a carbon source.
28318250	6	11	contain	have	935:938	arg2	activity					960:967	selective prebiotic activity	940:967	selective prebiotic activity toward specific bacterial strains able to deconstruct the glycosidic bonds and utilize them as a carbon source	940:1078	The diversity of oligosaccharides composition found in these coffee samples suggests that they could have selective prebiotic activity toward specific bacterial strains able to deconstruct the glycosidic bonds and utilize them as a carbon source.
28318250	4	12	with	monosaccharides	584:598	arg1	degree					515:520	a degree	513:520	a degree of polymerization ranging from 3 to 15	513:559	Oligosaccharides with a degree of polymerization ranging from 3 to 15, and their constituent monosaccharides, were characterized and quantified.
28318250	3	13	theme	spent	469:473	arg1	grounds					482:488	spent coffee grounds	469:488	spent coffee grounds	469:488	We investigated the oligosaccharides and their monomeric composition in dark roasted coffee beans, brewed coffee, and spent coffee grounds.
28318250	5	14	dep	hexoses	680:686	arg1	manno-oligosaccharides					730:751	manno-oligosaccharides	730:751	manno-oligosaccharides	730:751	The oligosaccharides identified were mainly hexoses (potentially galacto-oligosaccharides and manno-oligosaccharides) containing a heterogeneous mixture of glucose, arabinose, xylose, and rhamnose.
28318250	5	14	dep	hexoses	680:686	arg1	galacto-oligosaccharides					701:724	galacto-oligosaccharides	701:724	galacto-oligosaccharides	701:724	The oligosaccharides identified were mainly hexoses (potentially galacto-oligosaccharides and manno-oligosaccharides) containing a heterogeneous mixture of glucose, arabinose, xylose, and rhamnose.
28318250	0	15	from	Oligosaccharides	51:66	arg1	Coffee					78:83	Brewed Coffee	71:83	Brewed Coffee	71:83	Chemical Characterization of Potentially Prebiotic Oligosaccharides in Brewed Coffee and Spent Coffee Grounds.
28318250	0	15	from	Oligosaccharides	51:66	arg1	Grounds					102:108	Spent Coffee Grounds	89:108	Spent Coffee Grounds	89:108	Chemical Characterization of Potentially Prebiotic Oligosaccharides in Brewed Coffee and Spent Coffee Grounds.
28318250	3	16	theme	brewed	450:455	arg1	coffee					457:462	brewed coffee	450:462	brewed coffee	450:462	We investigated the oligosaccharides and their monomeric composition in dark roasted coffee beans, brewed coffee, and spent coffee grounds.
28318250	3	17	theme	coffee	436:441	arg1	beans					443:447	dark roasted coffee beans	423:447	dark roasted coffee beans	423:447	We investigated the oligosaccharides and their monomeric composition in dark roasted coffee beans, brewed coffee, and spent coffee grounds.
28318250	3	18	theme	coffee	475:480	arg1	grounds					482:488	spent coffee grounds	469:488	spent coffee grounds	469:488	We investigated the oligosaccharides and their monomeric composition in dark roasted coffee beans, brewed coffee, and spent coffee grounds.
28318250	3	19	theme	roasted	428:434	arg1	beans					443:447	dark roasted coffee beans	423:447	dark roasted coffee beans	423:447	We investigated the oligosaccharides and their monomeric composition in dark roasted coffee beans, brewed coffee, and spent coffee grounds.
28318250	1	20	from	present	166:172	arg1	plants					204:209	some plants	199:209	some plants	199:209	Oligosaccharides are indigestible carbohydrates widely present in mammalian milk and in some plants.
28318250	1	20	from	present	166:172	arg1	milk					187:190	mammalian milk	177:190	mammalian milk	177:190	Oligosaccharides are indigestible carbohydrates widely present in mammalian milk and in some plants.
28318250	6	21	theme	bacterial	985:993	arg1	strains					995:1001	specific bacterial strains	976:1001	specific bacterial strains able to deconstruct the glycosidic bonds and utilize them as a carbon source	976:1078	The diversity of oligosaccharides composition found in these coffee samples suggests that they could have selective prebiotic activity toward specific bacterial strains able to deconstruct the glycosidic bonds and utilize them as a carbon source.
28318250	0	22	from	Characterization	9:24	arg1	Coffee					78:83	Brewed Coffee	71:83	Brewed Coffee	71:83	Chemical Characterization of Potentially Prebiotic Oligosaccharides in Brewed Coffee and Spent Coffee Grounds.
28318250	0	22	from	Characterization	9:24	arg1	Grounds					102:108	Spent Coffee Grounds	89:108	Spent Coffee Grounds	89:108	Chemical Characterization of Potentially Prebiotic Oligosaccharides in Brewed Coffee and Spent Coffee Grounds.
28318250	6	23	theme	carbon	1066:1071	arg1	source					1073:1078	a carbon source	1064:1078	a carbon source	1064:1078	The diversity of oligosaccharides composition found in these coffee samples suggests that they could have selective prebiotic activity toward specific bacterial strains able to deconstruct the glycosidic bonds and utilize them as a carbon source.
28318250	6	23	theme	carbon	1066:1071	arg1	them					1056:1059	them	1056:1059	them	1056:1059	The diversity of oligosaccharides composition found in these coffee samples suggests that they could have selective prebiotic activity toward specific bacterial strains able to deconstruct the glycosidic bonds and utilize them as a carbon source.
28318250	0	24	from	Grounds	102:108	arg1	Characterization					9:24	Chemical Characterization	0:24	Chemical Characterization of Potentially Prebiotic Oligosaccharides in Brewed Coffee and Spent Coffee Grounds.	0:109	Chemical Characterization of Potentially Prebiotic Oligosaccharides in Brewed Coffee and Spent Coffee Grounds.
28318250	6	25	theme	oligosaccharides	851:866	arg1	composition					868:878	oligosaccharides composition	851:878	oligosaccharides composition	851:878	The diversity of oligosaccharides composition found in these coffee samples suggests that they could have selective prebiotic activity toward specific bacterial strains able to deconstruct the glycosidic bonds and utilize them as a carbon source.
28318250	4	26	theme	constituent	572:582	arg1	monosaccharides					584:598	their constituent monosaccharides	566:598	their constituent monosaccharides	566:598	Oligosaccharides with a degree of polymerization ranging from 3 to 15, and their constituent monosaccharides, were characterized and quantified.
28318250	6	27	theme	able	1003:1006	arg1	strains					995:1001	specific bacterial strains	976:1001	specific bacterial strains able to deconstruct the glycosidic bonds and utilize them as a carbon source	976:1078	The diversity of oligosaccharides composition found in these coffee samples suggests that they could have selective prebiotic activity toward specific bacterial strains able to deconstruct the glycosidic bonds and utilize them as a carbon source.
28318250	3	28	theme	dark	423:426	arg1	beans					443:447	dark roasted coffee beans	423:447	dark roasted coffee beans	423:447	We investigated the oligosaccharides and their monomeric composition in dark roasted coffee beans, brewed coffee, and spent coffee grounds.
28318250	5	29	theme	glucose	792:798	arg1	mixture					781:787	a heterogeneous mixture	765:787	a heterogeneous mixture of glucose, arabinose, xylose, and rhamnose	765:831	The oligosaccharides identified were mainly hexoses (potentially galacto-oligosaccharides and manno-oligosaccharides) containing a heterogeneous mixture of glucose, arabinose, xylose, and rhamnose.
28318250	2	30	from	oligosaccharides	289:304	arg1	coffee					309:314	coffee	309:314	coffee	309:314	Milk oligosaccharides are associated with positive health outcomes; however, oligosaccharides in coffee have not been extensively studied.
28318250	0	31	theme	Oligosaccharides	51:66	arg1	Characterization					9:24	Chemical Characterization	0:24	Chemical Characterization of Potentially Prebiotic Oligosaccharides in Brewed Coffee and Spent Coffee Grounds.	0:109	Chemical Characterization of Potentially Prebiotic Oligosaccharides in Brewed Coffee and Spent Coffee Grounds.
28318250	6	32	theme	specific	976:983	arg1	strains					995:1001	specific bacterial strains	976:1001	specific bacterial strains able to deconstruct the glycosidic bonds and utilize them as a carbon source	976:1078	The diversity of oligosaccharides composition found in these coffee samples suggests that they could have selective prebiotic activity toward specific bacterial strains able to deconstruct the glycosidic bonds and utilize them as a carbon source.
28318250	6	33	theme	prebiotic	950:958	arg1	activity					960:967	selective prebiotic activity	940:967	selective prebiotic activity toward specific bacterial strains able to deconstruct the glycosidic bonds and utilize them as a carbon source	940:1078	The diversity of oligosaccharides composition found in these coffee samples suggests that they could have selective prebiotic activity toward specific bacterial strains able to deconstruct the glycosidic bonds and utilize them as a carbon source.
28318250	0	34	theme	Prebiotic	41:49	arg1	Oligosaccharides					51:66	Potentially Prebiotic Oligosaccharides	29:66	Potentially Prebiotic Oligosaccharides in Brewed Coffee and Spent Coffee Grounds	29:108	Chemical Characterization of Potentially Prebiotic Oligosaccharides in Brewed Coffee and Spent Coffee Grounds.
28318250	3	35	dep	oligosaccharides	371:386	arg1	the					367:369	the	367:369	the	367:369	We investigated the oligosaccharides and their monomeric composition in dark roasted coffee beans, brewed coffee, and spent coffee grounds.
28318250	6	36	theme	selective	940:948	arg1	activity					960:967	selective prebiotic activity	940:967	selective prebiotic activity toward specific bacterial strains able to deconstruct the glycosidic bonds and utilize them as a carbon source	940:1078	The diversity of oligosaccharides composition found in these coffee samples suggests that they could have selective prebiotic activity toward specific bacterial strains able to deconstruct the glycosidic bonds and utilize them as a carbon source.
28318250	2	37	theme	health	263:268	arg1	outcomes					270:277	positive health outcomes	254:277	positive health outcomes	254:277	Milk oligosaccharides are associated with positive health outcomes; however, oligosaccharides in coffee have not been extensively studied.
28318250	6	38	theme	glycosidic	1027:1036	arg1	bonds					1038:1042	the glycosidic bonds	1023:1042	the glycosidic bonds	1023:1042	The diversity of oligosaccharides composition found in these coffee samples suggests that they could have selective prebiotic activity toward specific bacterial strains able to deconstruct the glycosidic bonds and utilize them as a carbon source.
28318250	0	39	theme	Brewed	71:76	arg1	Coffee					78:83	Brewed Coffee	71:83	Brewed Coffee	71:83	Chemical Characterization of Potentially Prebiotic Oligosaccharides in Brewed Coffee and Spent Coffee Grounds.
28318250	0	40	from	Coffee	78:83	arg1	Characterization					9:24	Chemical Characterization	0:24	Chemical Characterization of Potentially Prebiotic Oligosaccharides in Brewed Coffee and Spent Coffee Grounds.	0:109	Chemical Characterization of Potentially Prebiotic Oligosaccharides in Brewed Coffee and Spent Coffee Grounds.
28318250	2	41	theme	positive	254:261	arg1	outcomes					270:277	positive health outcomes	254:277	positive health outcomes	254:277	Milk oligosaccharides are associated with positive health outcomes; however, oligosaccharides in coffee have not been extensively studied.
28318250	4	42	theme	polymerization	525:538	arg1	degree					515:520	a degree	513:520	a degree of polymerization ranging from 3 to 15	513:559	Oligosaccharides with a degree of polymerization ranging from 3 to 15, and their constituent monosaccharides, were characterized and quantified.
28318250	1	43	theme	indigestible	132:143	arg1	Oligosaccharides					111:126	Oligosaccharides	111:126	Oligosaccharides	111:126	Oligosaccharides are indigestible carbohydrates widely present in mammalian milk and in some plants.
28318250	1	43	theme	indigestible	132:143	arg1	carbohydrates					145:157	indigestible carbohydrates	132:157	indigestible carbohydrates widely present in mammalian milk and in some plants	132:209	Oligosaccharides are indigestible carbohydrates widely present in mammalian milk and in some plants.
28318250	5	44	theme	arabinose	801:809	arg1	mixture					781:787	a heterogeneous mixture	765:787	a heterogeneous mixture of glucose, arabinose, xylose, and rhamnose	765:831	The oligosaccharides identified were mainly hexoses (potentially galacto-oligosaccharides and manno-oligosaccharides) containing a heterogeneous mixture of glucose, arabinose, xylose, and rhamnose.
28318250	5	45	contain	containing	754:763	arg1	oligosaccharides					640:655	The oligosaccharides	636:655	The oligosaccharides identified	636:666	The oligosaccharides identified were mainly hexoses (potentially galacto-oligosaccharides and manno-oligosaccharides) containing a heterogeneous mixture of glucose, arabinose, xylose, and rhamnose.
28318250	5	45	contain	containing	754:763	arg2	mixture					781:787	a heterogeneous mixture	765:787	a heterogeneous mixture of glucose, arabinose, xylose, and rhamnose	765:831	The oligosaccharides identified were mainly hexoses (potentially galacto-oligosaccharides and manno-oligosaccharides) containing a heterogeneous mixture of glucose, arabinose, xylose, and rhamnose.
28318250	5	45	contain	containing	754:763	arg1	hexoses					680:686	hexoses	680:686	hexoses (potentially galacto-oligosaccharides and manno-oligosaccharides) containing a heterogeneous mixture of glucose, arabinose, xylose, and rhamnose	680:831	The oligosaccharides identified were mainly hexoses (potentially galacto-oligosaccharides and manno-oligosaccharides) containing a heterogeneous mixture of glucose, arabinose, xylose, and rhamnose.
28318250	5	46	theme	heterogeneous	767:779	arg1	mixture					781:787	a heterogeneous mixture	765:787	a heterogeneous mixture of glucose, arabinose, xylose, and rhamnose	765:831	The oligosaccharides identified were mainly hexoses (potentially galacto-oligosaccharides and manno-oligosaccharides) containing a heterogeneous mixture of glucose, arabinose, xylose, and rhamnose.
28318250	1	47	from	plants	204:209	arg1	present					166:172	present	166:172	present	166:172	Oligosaccharides are indigestible carbohydrates widely present in mammalian milk and in some plants.
28318250	1	48	theme	present	166:172	arg1	Oligosaccharides					111:126	Oligosaccharides	111:126	Oligosaccharides	111:126	Oligosaccharides are indigestible carbohydrates widely present in mammalian milk and in some plants.
28318250	1	48	theme	present	166:172	arg1	carbohydrates					145:157	indigestible carbohydrates	132:157	indigestible carbohydrates widely present in mammalian milk and in some plants	132:209	Oligosaccharides are indigestible carbohydrates widely present in mammalian milk and in some plants.
28318250	5	49	theme	rhamnose	824:831	arg1	mixture					781:787	a heterogeneous mixture	765:787	a heterogeneous mixture of glucose, arabinose, xylose, and rhamnose	765:831	The oligosaccharides identified were mainly hexoses (potentially galacto-oligosaccharides and manno-oligosaccharides) containing a heterogeneous mixture of glucose, arabinose, xylose, and rhamnose.
28318250	2	50	theme	Milk	212:215	arg1	oligosaccharides					217:232	Milk oligosaccharides	212:232	Milk oligosaccharides	212:232	Milk oligosaccharides are associated with positive health outcomes; however, oligosaccharides in coffee have not been extensively studied.
28318250	4	51	with	Oligosaccharides	491:506	arg1	degree					515:520	a degree	513:520	a degree of polymerization ranging from 3 to 15	513:559	Oligosaccharides with a degree of polymerization ranging from 3 to 15, and their constituent monosaccharides, were characterized and quantified.
28318250	6	52	theme	composition	868:878	arg1	diversity					838:846	The diversity	834:846	The diversity of oligosaccharides composition found in these coffee samples	834:908	The diversity of oligosaccharides composition found in these coffee samples suggests that they could have selective prebiotic activity toward specific bacterial strains able to deconstruct the glycosidic bonds and utilize them as a carbon source.
28318250	6	53	located	found	880:884	arg2	diversity					838:846	The diversity	834:846	The diversity of oligosaccharides composition found in these coffee samples	834:908	The diversity of oligosaccharides composition found in these coffee samples suggests that they could have selective prebiotic activity toward specific bacterial strains able to deconstruct the glycosidic bonds and utilize them as a carbon source.
28318250	6	53	located	found	880:884	arg1	samples					902:908	these coffee samples	889:908	these coffee samples	889:908	The diversity of oligosaccharides composition found in these coffee samples suggests that they could have selective prebiotic activity toward specific bacterial strains able to deconstruct the glycosidic bonds and utilize them as a carbon source.
28318250	1	54	theme	mammalian	177:185	arg1	milk					187:190	mammalian milk	177:190	mammalian milk	177:190	Oligosaccharides are indigestible carbohydrates widely present in mammalian milk and in some plants.
24582240	7	0	theme	XnHAp	1120:1124	arg1	nanoparticles					1137:1149	XnHAp or XnHApSr nanoparticles	1120:1149	adding XnHAp or XnHApSr nanoparticles to xanthan hydrogel	1113:1169	Moreover, adding XnHAp or XnHApSr nanoparticles to xanthan hydrogel did not influence its porous morphology, gel content and swelling ratio.
24582240	3	1	theme	zeta	523:526	arg1	values					538:543	the zeta potential values	519:543	the zeta potential values	519:543	Xanthan chains enriched the surface of XnHAp and XnHApSr particles, increasing the zeta potential values from -(7±1)mV, determined for HAp, to -(17±3)mV and -(25±3)mV, respectively.
24582240	3	2	theme	potential	528:536	arg1	values					538:543	the zeta potential values	519:543	the zeta potential values	519:543	Xanthan chains enriched the surface of XnHAp and XnHApSr particles, increasing the zeta potential values from -(7±1)mV, determined for HAp, to -(17±3)mV and -(25±3)mV, respectively.
24582240	8	3	theme	alkaline	1380:1387	arg1	phosphatase					1389:1399	alkaline phosphatase	1380:1399	higher alkaline phosphatase activity	1373:1408	XCA/XnHAp and XCA/XnHApSr composites proved to be suitable for osteoblast growth and particularly XCA/XnHapSr composites induced higher alkaline phosphatase activity.
24582240	4	4	theme	scanning	773:780	arg1	microscopy					791:800	scanning electron microscopy	773:800	scanning electron microscopy	773:800	This effect led to high colloidal stability of XnHAp and XnHApSr dispersions and acicular particles (140±10)nm long and (8±2)nm wide, as determined by scanning electron microscopy and atomic force microscopy.
24582240	6	5	theme	comparable	1047:1056	arg1	values					1040:1045	break values	1034:1045	break values comparable to those determined for bare xanthan matrix	1034:1100	Dried nanocomposites presented surface energy, Young's modulus and stress at break values comparable to those determined for bare xanthan matrix.
24582240	7	6	theme	adding	1113:1118	arg1	nanoparticles					1137:1149	XnHAp or XnHApSr nanoparticles	1120:1149	adding XnHAp or XnHApSr nanoparticles to xanthan hydrogel	1113:1169	Moreover, adding XnHAp or XnHApSr nanoparticles to xanthan hydrogel did not influence its porous morphology, gel content and swelling ratio.
24582240	6	7	theme	xanthan	1087:1093	arg1	matrix					1095:1100	bare xanthan matrix	1082:1100	bare xanthan matrix	1082:1100	Dried nanocomposites presented surface energy, Young's modulus and stress at break values comparable to those determined for bare xanthan matrix.
24582240	7	8	theme	xanthan	1154:1160	arg1	hydrogel					1162:1169	xanthan hydrogel	1154:1169	xanthan hydrogel	1154:1169	Moreover, adding XnHAp or XnHApSr nanoparticles to xanthan hydrogel did not influence its porous morphology, gel content and swelling ratio.
24582240	4	9	theme	dispersions	687:697	arg1	stability					656:664	high colloidal stability	641:664	high colloidal stability of XnHAp and XnHApSr dispersions and acicular particles (140±10)nm long and (8±2)nm wide	641:753	This effect led to high colloidal stability of XnHAp and XnHApSr dispersions and acicular particles (140±10)nm long and (8±2)nm wide, as determined by scanning electron microscopy and atomic force microscopy.
24582240	4	10	theme	colloidal	646:654	arg1	stability					656:664	high colloidal stability	641:664	high colloidal stability of XnHAp and XnHApSr dispersions and acicular particles (140±10)nm long and (8±2)nm wide	641:753	This effect led to high colloidal stability of XnHAp and XnHApSr dispersions and acicular particles (140±10)nm long and (8±2)nm wide, as determined by scanning electron microscopy and atomic force microscopy.
24582240	5	11	dep	nanocomposites	913:926	arg1	XCA/XnHAp					929:937	XCA/XnHAp	929:937	XCA/XnHAp	929:937	XnHAp and XnHApSr particles were added to xanthan hydrogels to produce compatible nanocomposites (XCA/XnHAp and XCA/XnHApSr).
24582240	5	11	dep	nanocomposites	913:926	arg1	nanocomposites					913:926	compatible nanocomposites	902:926	compatible nanocomposites (XCA/XnHAp and XCA/XnHApSr)	902:954	XnHAp and XnHApSr particles were added to xanthan hydrogels to produce compatible nanocomposites (XCA/XnHAp and XCA/XnHApSr).
24582240	5	11	dep	nanocomposites	913:926	arg1	XCA/XnHApSr					943:953	XCA/XnHApSr	943:953	XCA/XnHApSr	943:953	XnHAp and XnHApSr particles were added to xanthan hydrogels to produce compatible nanocomposites (XCA/XnHAp and XCA/XnHApSr).
24582240	4	12	theme	particles	712:720	arg1	stability					656:664	high colloidal stability	641:664	high colloidal stability of XnHAp and XnHApSr dispersions and acicular particles (140±10)nm long and (8±2)nm wide	641:753	This effect led to high colloidal stability of XnHAp and XnHApSr dispersions and acicular particles (140±10)nm long and (8±2)nm wide, as determined by scanning electron microscopy and atomic force microscopy.
24582240	6	13	theme	Dried	957:961	arg1	nanocomposites					963:976	Dried nanocomposites	957:976	Dried nanocomposites	957:976	Dried nanocomposites presented surface energy, Young's modulus and stress at break values comparable to those determined for bare xanthan matrix.
24582240	6	14	attach	presented	978:986	arg2	nanocomposites					963:976	Dried nanocomposites	957:976	Dried nanocomposites	957:976	Dried nanocomposites presented surface energy, Young's modulus and stress at break values comparable to those determined for bare xanthan matrix.
24582240	6	14	attach	presented	978:986	arg1	values					1040:1045	break values	1034:1045	break values comparable to those determined for bare xanthan matrix	1034:1100	Dried nanocomposites presented surface energy, Young's modulus and stress at break values comparable to those determined for bare xanthan matrix.
24582240	4	15	theme	acicular	703:710	arg1	140±10					723:728	140±10	723:728	140±10	723:728	This effect led to high colloidal stability of XnHAp and XnHApSr dispersions and acicular particles (140±10)nm long and (8±2)nm wide, as determined by scanning electron microscopy and atomic force microscopy.
24582240	4	15	theme	acicular	703:710	arg1	particles					712:720	acicular particles	703:720	acicular particles (140±10)	703:729	This effect led to high colloidal stability of XnHAp and XnHApSr dispersions and acicular particles (140±10)nm long and (8±2)nm wide, as determined by scanning electron microscopy and atomic force microscopy.
24582240	6	16	theme	Young	1004:1008	arg1	modulus					1012:1018	Young's modulus	1004:1018	Young's modulus	1004:1018	Dried nanocomposites presented surface energy, Young's modulus and stress at break values comparable to those determined for bare xanthan matrix.
24582240	4	17	theme	XnHAp	669:673	arg1	dispersions					687:697	XnHAp and XnHApSr dispersions	669:697	XnHAp and XnHApSr dispersions	669:697	This effect led to high colloidal stability of XnHAp and XnHApSr dispersions and acicular particles (140±10)nm long and (8±2)nm wide, as determined by scanning electron microscopy and atomic force microscopy.
24582240	6	18	theme	surface	988:994	arg1	energy					996:1001	surface energy	988:1001	surface energy	988:1001	Dried nanocomposites presented surface energy, Young's modulus and stress at break values comparable to those determined for bare xanthan matrix.
24582240	4	19	theme	wide	750:753	arg1	dispersions					687:697	XnHAp and XnHApSr dispersions	669:697	XnHAp and XnHApSr dispersions	669:697	This effect led to high colloidal stability of XnHAp and XnHApSr dispersions and acicular particles (140±10)nm long and (8±2)nm wide, as determined by scanning electron microscopy and atomic force microscopy.
24582240	5	20	theme	XnHApSr	841:847	arg1	particles					849:857	XnHAp and XnHApSr particles	831:857	XnHAp and XnHApSr particles	831:857	XnHAp and XnHApSr particles were added to xanthan hydrogels to produce compatible nanocomposites (XCA/XnHAp and XCA/XnHApSr).
24582240	7	21	theme	swelling	1228:1235	arg1	ratio					1237:1241	swelling ratio	1228:1241	swelling ratio	1228:1241	Moreover, adding XnHAp or XnHApSr nanoparticles to xanthan hydrogel did not influence its porous morphology, gel content and swelling ratio.
24582240	4	22	dep	nm	747:748	arg1	8±2					743:745	8±2	743:745	8±2	743:745	This effect led to high colloidal stability of XnHAp and XnHApSr dispersions and acicular particles (140±10)nm long and (8±2)nm wide, as determined by scanning electron microscopy and atomic force microscopy.
24582240	1	23	theme	conventional	311:322	arg1	HAp					350:352	HAp	350:352	HAp	350:352	In this work xanthan-nanohydroxyapatite (XnHAp) and its equivalent strontium substituted (XnHApSr) were synthesized by the precipitation of nanohydroxyapatite in xanthan aqueous solution, characterized and compared to conventional hydroxyapatite particles (HAp).
24582240	1	23	theme	conventional	311:322	arg1	particles					339:347	conventional hydroxyapatite particles	311:347	conventional hydroxyapatite particles (HAp)	311:353	In this work xanthan-nanohydroxyapatite (XnHAp) and its equivalent strontium substituted (XnHApSr) were synthesized by the precipitation of nanohydroxyapatite in xanthan aqueous solution, characterized and compared to conventional hydroxyapatite particles (HAp).
24582240	5	24	theme	XnHAp	831:835	arg1	particles					849:857	XnHAp and XnHApSr particles	831:857	XnHAp and XnHApSr particles	831:857	XnHAp and XnHApSr particles were added to xanthan hydrogels to produce compatible nanocomposites (XCA/XnHAp and XCA/XnHApSr).
24582240	4	25	theme	XnHApSr	679:685	arg1	dispersions					687:697	XnHAp and XnHApSr dispersions	669:697	XnHAp and XnHApSr dispersions	669:697	This effect led to high colloidal stability of XnHAp and XnHApSr dispersions and acicular particles (140±10)nm long and (8±2)nm wide, as determined by scanning electron microscopy and atomic force microscopy.
24582240	2	26	theme	X-ray	421:425	arg1	diffraction					427:437	X-ray diffraction	421:437	X-ray diffraction	421:437	XnHAp and XnHApSr were less crystalline than HAp, as revealed by X-ray diffraction.
24582240	1	27	theme	hydroxyapatite	324:337	arg1	HAp					350:352	HAp	350:352	HAp	350:352	In this work xanthan-nanohydroxyapatite (XnHAp) and its equivalent strontium substituted (XnHApSr) were synthesized by the precipitation of nanohydroxyapatite in xanthan aqueous solution, characterized and compared to conventional hydroxyapatite particles (HAp).
24582240	1	27	theme	hydroxyapatite	324:337	arg1	particles					339:347	conventional hydroxyapatite particles	311:347	conventional hydroxyapatite particles (HAp)	311:353	In this work xanthan-nanohydroxyapatite (XnHAp) and its equivalent strontium substituted (XnHApSr) were synthesized by the precipitation of nanohydroxyapatite in xanthan aqueous solution, characterized and compared to conventional hydroxyapatite particles (HAp).
24582240	4	28	theme	force	813:817	arg1	microscopy					819:828	atomic force microscopy	806:828	atomic force microscopy	806:828	This effect led to high colloidal stability of XnHAp and XnHApSr dispersions and acicular particles (140±10)nm long and (8±2)nm wide, as determined by scanning electron microscopy and atomic force microscopy.
24582240	1	29	theme	nanohydroxyapatite	233:250	arg1	precipitation					216:228	the precipitation	212:228	the precipitation of nanohydroxyapatite	212:250	In this work xanthan-nanohydroxyapatite (XnHAp) and its equivalent strontium substituted (XnHApSr) were synthesized by the precipitation of nanohydroxyapatite in xanthan aqueous solution, characterized and compared to conventional hydroxyapatite particles (HAp).
24582240	1	30	dep	substituted	170:180	arg1	strontium					160:168	its equivalent strontium	145:168	its equivalent strontium substituted (XnHApSr)	145:190	In this work xanthan-nanohydroxyapatite (XnHAp) and its equivalent strontium substituted (XnHApSr) were synthesized by the precipitation of nanohydroxyapatite in xanthan aqueous solution, characterized and compared to conventional hydroxyapatite particles (HAp).
24582240	1	30	dep	substituted	170:180	arg1	XnHApSr					183:189	XnHApSr	183:189	XnHApSr	183:189	In this work xanthan-nanohydroxyapatite (XnHAp) and its equivalent strontium substituted (XnHApSr) were synthesized by the precipitation of nanohydroxyapatite in xanthan aqueous solution, characterized and compared to conventional hydroxyapatite particles (HAp).
24582240	3	31	theme	XnHApSr	489:495	arg1	particles					497:505	XnHApSr particles	489:505	XnHApSr particles	489:505	Xanthan chains enriched the surface of XnHAp and XnHApSr particles, increasing the zeta potential values from -(7±1)mV, determined for HAp, to -(17±3)mV and -(25±3)mV, respectively.
24582240	7	32	theme	porous	1193:1198	arg1	morphology					1200:1209	its porous morphology	1189:1209	its porous morphology	1189:1209	Moreover, adding XnHAp or XnHApSr nanoparticles to xanthan hydrogel did not influence its porous morphology, gel content and swelling ratio.
24582240	8	33	theme	higher	1373:1378	arg1	activity					1401:1408	higher alkaline phosphatase activity	1373:1408	higher alkaline phosphatase activity	1373:1408	XCA/XnHAp and XCA/XnHApSr composites proved to be suitable for osteoblast growth and particularly XCA/XnHapSr composites induced higher alkaline phosphatase activity.
24582240	8	34	theme	osteoblast	1307:1316	arg1	growth					1318:1323	osteoblast growth	1307:1323	osteoblast growth	1307:1323	XCA/XnHAp and XCA/XnHApSr composites proved to be suitable for osteoblast growth and particularly XCA/XnHapSr composites induced higher alkaline phosphatase activity.
24582240	0	35	theme	xanthan-hydroxyapatite	34:55	arg1	characterization					14:29	characterization	14:29	characterization	14:29	Synthesis and characterization of xanthan-hydroxyapatite nanocomposites for cellular uptake.
24582240	0	35	theme	xanthan-hydroxyapatite	34:55	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis and characterization of xanthan-hydroxyapatite nanocomposites for cellular uptake.
24582240	3	36	theme	Xanthan	440:446	arg1	chains					448:453	Xanthan chains	440:453	Xanthan chains	440:453	Xanthan chains enriched the surface of XnHAp and XnHApSr particles, increasing the zeta potential values from -(7±1)mV, determined for HAp, to -(17±3)mV and -(25±3)mV, respectively.
24582240	4	37	theme	long	733:736	arg1	dispersions					687:697	XnHAp and XnHApSr dispersions	669:697	XnHAp and XnHApSr dispersions	669:697	This effect led to high colloidal stability of XnHAp and XnHApSr dispersions and acicular particles (140±10)nm long and (8±2)nm wide, as determined by scanning electron microscopy and atomic force microscopy.
24582240	3	38	theme	XnHAp	479:483	arg1	surface					468:474	the surface	464:474	the surface of XnHAp and XnHApSr particles	464:505	Xanthan chains enriched the surface of XnHAp and XnHApSr particles, increasing the zeta potential values from -(7±1)mV, determined for HAp, to -(17±3)mV and -(25±3)mV, respectively.
24582240	0	39	theme	cellular	76:83	arg1	uptake					85:90	cellular uptake	76:90	cellular uptake	76:90	Synthesis and characterization of xanthan-hydroxyapatite nanocomposites for cellular uptake.
24582240	8	40	theme	phosphatase	1389:1399	arg1	activity					1401:1408	higher alkaline phosphatase activity	1373:1408	higher alkaline phosphatase activity	1373:1408	XCA/XnHAp and XCA/XnHApSr composites proved to be suitable for osteoblast growth and particularly XCA/XnHapSr composites induced higher alkaline phosphatase activity.
24582240	4	41	theme	high	641:644	arg1	stability					656:664	high colloidal stability	641:664	high colloidal stability of XnHAp and XnHApSr dispersions and acicular particles (140±10)nm long and (8±2)nm wide	641:753	This effect led to high colloidal stability of XnHAp and XnHApSr dispersions and acicular particles (140±10)nm long and (8±2)nm wide, as determined by scanning electron microscopy and atomic force microscopy.
24582240	4	42	theme	electron	782:789	arg1	microscopy					791:800	scanning electron microscopy	773:800	scanning electron microscopy	773:800	This effect led to high colloidal stability of XnHAp and XnHApSr dispersions and acicular particles (140±10)nm long and (8±2)nm wide, as determined by scanning electron microscopy and atomic force microscopy.
24582240	6	43	theme	break	1034:1038	arg1	values					1040:1045	break values	1034:1045	break values comparable to those determined for bare xanthan matrix	1034:1100	Dried nanocomposites presented surface energy, Young's modulus and stress at break values comparable to those determined for bare xanthan matrix.
24582240	4	44	theme	atomic	806:811	arg1	microscopy					819:828	atomic force microscopy	806:828	atomic force microscopy	806:828	This effect led to high colloidal stability of XnHAp and XnHApSr dispersions and acicular particles (140±10)nm long and (8±2)nm wide, as determined by scanning electron microscopy and atomic force microscopy.
24582240	8	45	theme	XCA/XnHapSr	1342:1352	arg1	composites					1354:1363	particularly XCA/XnHapSr composites	1329:1363	particularly XCA/XnHapSr composites	1329:1363	XCA/XnHAp and XCA/XnHApSr composites proved to be suitable for osteoblast growth and particularly XCA/XnHapSr composites induced higher alkaline phosphatase activity.
24582240	3	46	theme	particles	497:505	arg1	surface					468:474	the surface	464:474	the surface of XnHAp and XnHApSr particles	464:505	Xanthan chains enriched the surface of XnHAp and XnHApSr particles, increasing the zeta potential values from -(7±1)mV, determined for HAp, to -(17±3)mV and -(25±3)mV, respectively.
24582240	8	47	theme	XCA/XnHApSr	1258:1268	arg1	composites					1270:1279	XCA/XnHAp and XCA/XnHApSr composites	1244:1279	XCA/XnHAp and XCA/XnHApSr composites	1244:1279	XCA/XnHAp and XCA/XnHApSr composites proved to be suitable for osteoblast growth and particularly XCA/XnHapSr composites induced higher alkaline phosphatase activity.
24582240	5	48	theme	xanthan	873:879	arg1	hydrogels					881:889	xanthan hydrogels	873:889	xanthan hydrogels	873:889	XnHAp and XnHApSr particles were added to xanthan hydrogels to produce compatible nanocomposites (XCA/XnHAp and XCA/XnHApSr).
24582240	1	49	theme	equivalent	149:158	arg1	strontium					160:168	its equivalent strontium	145:168	its equivalent strontium substituted (XnHApSr)	145:190	In this work xanthan-nanohydroxyapatite (XnHAp) and its equivalent strontium substituted (XnHApSr) were synthesized by the precipitation of nanohydroxyapatite in xanthan aqueous solution, characterized and compared to conventional hydroxyapatite particles (HAp).
24582240	1	49	theme	equivalent	149:158	arg1	XnHApSr					183:189	XnHApSr	183:189	XnHApSr	183:189	In this work xanthan-nanohydroxyapatite (XnHAp) and its equivalent strontium substituted (XnHApSr) were synthesized by the precipitation of nanohydroxyapatite in xanthan aqueous solution, characterized and compared to conventional hydroxyapatite particles (HAp).
24582240	8	50	theme	XCA/XnHAp	1244:1252	arg1	composites					1270:1279	XCA/XnHAp and XCA/XnHApSr composites	1244:1279	XCA/XnHAp and XCA/XnHApSr composites	1244:1279	XCA/XnHAp and XCA/XnHApSr composites proved to be suitable for osteoblast growth and particularly XCA/XnHapSr composites induced higher alkaline phosphatase activity.
24582240	1	51	theme	xanthan	255:261	arg1	solution					271:278	xanthan aqueous solution	255:278	xanthan aqueous solution	255:278	In this work xanthan-nanohydroxyapatite (XnHAp) and its equivalent strontium substituted (XnHApSr) were synthesized by the precipitation of nanohydroxyapatite in xanthan aqueous solution, characterized and compared to conventional hydroxyapatite particles (HAp).
24582240	7	52	theme	XnHApSr	1129:1135	arg1	nanoparticles					1137:1149	XnHAp or XnHApSr nanoparticles	1120:1149	adding XnHAp or XnHApSr nanoparticles to xanthan hydrogel	1113:1169	Moreover, adding XnHAp or XnHApSr nanoparticles to xanthan hydrogel did not influence its porous morphology, gel content and swelling ratio.
24582240	3	53	dep	mV	590:591	arg1	17±3					585:588	17±3	585:588	17±3	585:588	Xanthan chains enriched the surface of XnHAp and XnHApSr particles, increasing the zeta potential values from -(7±1)mV, determined for HAp, to -(17±3)mV and -(25±3)mV, respectively.
24582240	5	54	theme	compatible	902:911	arg1	XCA/XnHAp					929:937	XCA/XnHAp	929:937	XCA/XnHAp	929:937	XnHAp and XnHApSr particles were added to xanthan hydrogels to produce compatible nanocomposites (XCA/XnHAp and XCA/XnHApSr).
24582240	5	54	theme	compatible	902:911	arg1	nanocomposites					913:926	compatible nanocomposites	902:926	compatible nanocomposites (XCA/XnHAp and XCA/XnHApSr)	902:954	XnHAp and XnHApSr particles were added to xanthan hydrogels to produce compatible nanocomposites (XCA/XnHAp and XCA/XnHApSr).
24582240	5	54	theme	compatible	902:911	arg1	XCA/XnHApSr					943:953	XCA/XnHApSr	943:953	XCA/XnHApSr	943:953	XnHAp and XnHApSr particles were added to xanthan hydrogels to produce compatible nanocomposites (XCA/XnHAp and XCA/XnHApSr).
24582240	7	55	theme	gel	1212:1214	arg1	content					1216:1222	gel content	1212:1222	gel content	1212:1222	Moreover, adding XnHAp or XnHApSr nanoparticles to xanthan hydrogel did not influence its porous morphology, gel content and swelling ratio.
24582240	3	56	dep	mV	556:557	arg1	7±1					552:554	7±1	552:554	7±1	552:554	Xanthan chains enriched the surface of XnHAp and XnHApSr particles, increasing the zeta potential values from -(7±1)mV, determined for HAp, to -(17±3)mV and -(25±3)mV, respectively.
24582240	6	57	theme	bare	1082:1085	arg1	matrix					1095:1100	bare xanthan matrix	1082:1100	bare xanthan matrix	1082:1100	Dried nanocomposites presented surface energy, Young's modulus and stress at break values comparable to those determined for bare xanthan matrix.
24582240	1	58	theme	aqueous	263:269	arg1	solution					271:278	xanthan aqueous solution	255:278	xanthan aqueous solution	255:278	In this work xanthan-nanohydroxyapatite (XnHAp) and its equivalent strontium substituted (XnHApSr) were synthesized by the precipitation of nanohydroxyapatite in xanthan aqueous solution, characterized and compared to conventional hydroxyapatite particles (HAp).
29042445	3	0	theme	fundamental	715:725	arg1	properties					727:736	the fundamental properties	711:736	the fundamental properties of OST activity	711:752	Trypanosoma brucei encodes three paralogue single-protein OSTs called TbSTT3A, TbSTT3B, and TbSTT3C that can functionally complement the Saccharomyces cerevisiae OST, making it an ideal experimental system to study the fundamental properties of OST activity.
29042445	4	1	from	forms	855:859	arg1	host					892:895	the heterologous expression host	864:895	the heterologous expression host S. cerevisiae where we were able to apply yeast genetic tools and newly developed glycoproteomics methods	864:1001	We characterized the LLO and polypeptide specificity of all three TbOST isoforms and their chimeric forms in the heterologous expression host S. cerevisiae where we were able to apply yeast genetic tools and newly developed glycoproteomics methods.
29042445	2	2	theme	nascent	371:377	arg1	chain					391:395	a nascent polypeptide chain	369:395	a nascent polypeptide chain	369:395	The transfer of a glycan from a lipid-linked oligosaccharide (LLO) donor to the asparagine residue of a nascent polypeptide chain is catalyzed by an oligosaccharyltransferase (OST) in the lumen of the endoplasmic reticulum (ER).
29042445	8	3	theme	molecular	1588:1596	arg1	mechanisms					1598:1607	the molecular mechanisms	1584:1607	the molecular mechanisms	1584:1607	These results provide a basis to further investigate the molecular mechanisms and contribution of single amino acids in OST interaction with its substrates.
29042445	5	4	theme	LLO	1239:1241	arg1	preference					1243:1252	any LLO preference	1235:1252	any LLO preference	1235:1252	We demonstrated that TbSTT3A accepted LLO substrates ranging from Man5GlcNAc2 to Man7GlcNAc2 In contrast, TbSTT3B required more complex precursors ranging from Man6GlcNAc2 to Glc3Man9GlcNAc2 structures, and TbSTT3C did not display any LLO preference.
29042445	4	5	theme	expression	881:890	arg1	host					892:895	the heterologous expression host	864:895	the heterologous expression host S. cerevisiae where we were able to apply yeast genetic tools and newly developed glycoproteomics methods	864:1001	We characterized the LLO and polypeptide specificity of all three TbOST isoforms and their chimeric forms in the heterologous expression host S. cerevisiae where we were able to apply yeast genetic tools and newly developed glycoproteomics methods.
29042445	4	6	from	specificity	796:806	arg1	host					892:895	the heterologous expression host	864:895	the heterologous expression host S. cerevisiae where we were able to apply yeast genetic tools and newly developed glycoproteomics methods	864:1001	We characterized the LLO and polypeptide specificity of all three TbOST isoforms and their chimeric forms in the heterologous expression host S. cerevisiae where we were able to apply yeast genetic tools and newly developed glycoproteomics methods.
29042445	5	7	theme	complex	1132:1138	arg1	precursors					1140:1149	more complex precursors	1127:1149	more complex precursors ranging from Man6GlcNAc2 to Glc3Man9GlcNAc2 structures	1127:1204	We demonstrated that TbSTT3A accepted LLO substrates ranging from Man5GlcNAc2 to Man7GlcNAc2 In contrast, TbSTT3B required more complex precursors ranging from Man6GlcNAc2 to Glc3Man9GlcNAc2 structures, and TbSTT3C did not display any LLO preference.
29042445	5	8	theme	Glc3Man9GlcNAc2	1179:1193	arg1	structures					1195:1204	Glc3Man9GlcNAc2 structures	1179:1204	Glc3Man9GlcNAc2 structures	1179:1204	We demonstrated that TbSTT3A accepted LLO substrates ranging from Man5GlcNAc2 to Man7GlcNAc2 In contrast, TbSTT3B required more complex precursors ranging from Man6GlcNAc2 to Glc3Man9GlcNAc2 structures, and TbSTT3C did not display any LLO preference.
29042445	6	9	theme	Sequence	1255:1262	arg1	differences					1264:1274	Sequence differences	1255:1274	Sequence differences between the isoforms	1255:1295	Sequence differences between the isoforms cluster in three distinct regions.
29042445	2	10	from	donor	334:338	arg1	transfer					271:278	The transfer	267:278	The transfer of a glycan from a lipid-linked oligosaccharide (LLO) donor to the asparagine residue of a nascent polypeptide chain	267:395	The transfer of a glycan from a lipid-linked oligosaccharide (LLO) donor to the asparagine residue of a nascent polypeptide chain is catalyzed by an oligosaccharyltransferase (OST) in the lumen of the endoplasmic reticulum (ER).
29042445	2	10	from	donor	334:338	arg1	glycan					285:290	a glycan	283:290	a glycan from a lipid-linked oligosaccharide (LLO) donor	283:338	The transfer of a glycan from a lipid-linked oligosaccharide (LLO) donor to the asparagine residue of a nascent polypeptide chain is catalyzed by an oligosaccharyltransferase (OST) in the lumen of the endoplasmic reticulum (ER).
29042445	0	11	theme	functional	93:102	arg1	expression					104:113	functional expression	93:113	functional expression of domain-swapped chimeras in yeast	93:149	Analysis of substrate specificity of Trypanosoma brucei oligosaccharyltransferases (OSTs) by functional expression of domain-swapped chimeras in yeast.
29042445	3	12	theme	OST	741:743	arg1	activity					745:752	OST activity	741:752	OST activity	741:752	Trypanosoma brucei encodes three paralogue single-protein OSTs called TbSTT3A, TbSTT3B, and TbSTT3C that can functionally complement the Saccharomyces cerevisiae OST, making it an ideal experimental system to study the fundamental properties of OST activity.
29042445	4	13	theme	LLO	776:778	arg1	specificity					796:806	the LLO and polypeptide specificity	772:806	specificity	796:806	We characterized the LLO and polypeptide specificity of all three TbOST isoforms and their chimeric forms in the heterologous expression host S. cerevisiae where we were able to apply yeast genetic tools and newly developed glycoproteomics methods.
29042445	8	14	from	contribution	1613:1624	arg1	interaction					1655:1665	OST interaction	1651:1665	OST interaction with its substrates	1651:1685	These results provide a basis to further investigate the molecular mechanisms and contribution of single amino acids in OST interaction with its substrates.
29042445	2	15	theme	oligosaccharide	312:326	arg1	donor					334:338	a lipid-linked oligosaccharide (LLO) donor	297:338	a lipid-linked oligosaccharide (LLO) donor	297:338	The transfer of a glycan from a lipid-linked oligosaccharide (LLO) donor to the asparagine residue of a nascent polypeptide chain is catalyzed by an oligosaccharyltransferase (OST) in the lumen of the endoplasmic reticulum (ER).
29042445	0	16	theme	domain-swapped	118:131	arg1	chimeras					133:140	domain-swapped chimeras	118:140	domain-swapped chimeras	118:140	Analysis of substrate specificity of Trypanosoma brucei oligosaccharyltransferases (OSTs) by functional expression of domain-swapped chimeras in yeast.
29042445	3	17	theme	activity	745:752	arg1	properties					727:736	the fundamental properties	711:736	the fundamental properties of OST activity	711:752	Trypanosoma brucei encodes three paralogue single-protein OSTs called TbSTT3A, TbSTT3B, and TbSTT3C that can functionally complement the Saccharomyces cerevisiae OST, making it an ideal experimental system to study the fundamental properties of OST activity.
29042445	6	18	theme	distinct	1314:1321	arg1	regions					1323:1329	three distinct regions	1308:1329	three distinct regions	1308:1329	Sequence differences between the isoforms cluster in three distinct regions.
29042445	2	19	theme	lipid-linked	299:310	arg1	oligosaccharide					312:326	a lipid-linked oligosaccharide	297:326	a lipid-linked oligosaccharide (LLO) donor	297:338	The transfer of a glycan from a lipid-linked oligosaccharide (LLO) donor to the asparagine residue of a nascent polypeptide chain is catalyzed by an oligosaccharyltransferase (OST) in the lumen of the endoplasmic reticulum (ER).
29042445	2	19	theme	lipid-linked	299:310	arg1	LLO					329:331	LLO	329:331	LLO	329:331	The transfer of a glycan from a lipid-linked oligosaccharide (LLO) donor to the asparagine residue of a nascent polypeptide chain is catalyzed by an oligosaccharyltransferase (OST) in the lumen of the endoplasmic reticulum (ER).
29042445	7	20	theme	OST	1389:1391	arg1	proteins					1393:1400	different OST proteins	1379:1400	different OST proteins	1379:1400	We have swapped the individual regions between different OST proteins and identified region 2 to influence the specificity toward the LLO and region 1 to influence polypeptide substrate specificity.
29042445	7	21	theme	polypeptide	1496:1506	arg1	specificity					1518:1528	polypeptide substrate specificity	1496:1528	polypeptide substrate specificity	1496:1528	We have swapped the individual regions between different OST proteins and identified region 2 to influence the specificity toward the LLO and region 1 to influence polypeptide substrate specificity.
29042445	4	22	theme	polypeptide	784:794	arg1	specificity					796:806	the LLO and polypeptide specificity	772:806	specificity	796:806	We characterized the LLO and polypeptide specificity of all three TbOST isoforms and their chimeric forms in the heterologous expression host S. cerevisiae where we were able to apply yeast genetic tools and newly developed glycoproteomics methods.
29042445	2	23	from	transfer	271:278	arg1	donor					334:338	a lipid-linked oligosaccharide (LLO) donor	297:338	a lipid-linked oligosaccharide (LLO) donor	297:338	The transfer of a glycan from a lipid-linked oligosaccharide (LLO) donor to the asparagine residue of a nascent polypeptide chain is catalyzed by an oligosaccharyltransferase (OST) in the lumen of the endoplasmic reticulum (ER).
29042445	1	24	theme	essential	189:197	arg1	glycosylation					169:181	N-Linked protein glycosylation	152:181	N-Linked protein glycosylation	152:181	N-Linked protein glycosylation is an essential and highly conserved post-translational modification in eukaryotes.
29042445	1	24	theme	essential	189:197	arg1	modification					239:250	an essential and highly conserved post-translational modification	186:250	an essential and highly conserved post-translational modification in eukaryotes	186:264	N-Linked protein glycosylation is an essential and highly conserved post-translational modification in eukaryotes.
29042445	3	25	theme	paralogue	529:537	arg1	OSTs					554:557	three paralogue single-protein OSTs	523:557	three paralogue single-protein OSTs called TbSTT3A, TbSTT3B, and TbSTT3C that can functionally complement the Saccharomyces cerevisiae OST	523:660	Trypanosoma brucei encodes three paralogue single-protein OSTs called TbSTT3A, TbSTT3B, and TbSTT3C that can functionally complement the Saccharomyces cerevisiae OST, making it an ideal experimental system to study the fundamental properties of OST activity.
29042445	3	25	theme	paralogue	529:537	arg1	brucei					508:513	Trypanosoma brucei	496:513	Trypanosoma brucei	496:513	Trypanosoma brucei encodes three paralogue single-protein OSTs called TbSTT3A, TbSTT3B, and TbSTT3C that can functionally complement the Saccharomyces cerevisiae OST, making it an ideal experimental system to study the fundamental properties of OST activity.
29042445	4	26	theme	heterologous	868:879	arg1	host					892:895	the heterologous expression host	864:895	the heterologous expression host S. cerevisiae where we were able to apply yeast genetic tools and newly developed glycoproteomics methods	864:1001	We characterized the LLO and polypeptide specificity of all three TbOST isoforms and their chimeric forms in the heterologous expression host S. cerevisiae where we were able to apply yeast genetic tools and newly developed glycoproteomics methods.
29042445	3	27	theme	single-protein	539:552	arg1	OSTs					554:557	three paralogue single-protein OSTs	523:557	three paralogue single-protein OSTs called TbSTT3A, TbSTT3B, and TbSTT3C that can functionally complement the Saccharomyces cerevisiae OST	523:660	Trypanosoma brucei encodes three paralogue single-protein OSTs called TbSTT3A, TbSTT3B, and TbSTT3C that can functionally complement the Saccharomyces cerevisiae OST, making it an ideal experimental system to study the fundamental properties of OST activity.
29042445	3	27	theme	single-protein	539:552	arg1	brucei					508:513	Trypanosoma brucei	496:513	Trypanosoma brucei	496:513	Trypanosoma brucei encodes three paralogue single-protein OSTs called TbSTT3A, TbSTT3B, and TbSTT3C that can functionally complement the Saccharomyces cerevisiae OST, making it an ideal experimental system to study the fundamental properties of OST activity.
29042445	2	28	link	lipid-linked	299:310	arg1	oligosaccharide					312:326	a lipid-linked oligosaccharide	297:326	a lipid-linked oligosaccharide (LLO) donor	297:338	The transfer of a glycan from a lipid-linked oligosaccharide (LLO) donor to the asparagine residue of a nascent polypeptide chain is catalyzed by an oligosaccharyltransferase (OST) in the lumen of the endoplasmic reticulum (ER).
29042445	2	28	link	lipid-linked	299:310	arg1	LLO					329:331	LLO	329:331	LLO	329:331	The transfer of a glycan from a lipid-linked oligosaccharide (LLO) donor to the asparagine residue of a nascent polypeptide chain is catalyzed by an oligosaccharyltransferase (OST) in the lumen of the endoplasmic reticulum (ER).
29042445	7	29	theme	different	1379:1387	arg1	proteins					1393:1400	different OST proteins	1379:1400	different OST proteins	1379:1400	We have swapped the individual regions between different OST proteins and identified region 2 to influence the specificity toward the LLO and region 1 to influence polypeptide substrate specificity.
29042445	0	30	theme	specificity	22:32	arg1	Analysis					0:7	Analysis	0:7	Analysis of substrate specificity of Trypanosoma brucei oligosaccharyltransferases (OSTs) by functional expression of domain-swapped chimeras in yeast.	0:150	Analysis of substrate specificity of Trypanosoma brucei oligosaccharyltransferases (OSTs) by functional expression of domain-swapped chimeras in yeast.
29042445	7	31	theme	substrate	1508:1516	arg1	specificity					1518:1528	polypeptide substrate specificity	1496:1528	polypeptide substrate specificity	1496:1528	We have swapped the individual regions between different OST proteins and identified region 2 to influence the specificity toward the LLO and region 1 to influence polypeptide substrate specificity.
29042445	1	32	theme	conserved	210:218	arg1	glycosylation					169:181	N-Linked protein glycosylation	152:181	N-Linked protein glycosylation	152:181	N-Linked protein glycosylation is an essential and highly conserved post-translational modification in eukaryotes.
29042445	1	32	theme	conserved	210:218	arg1	modification					239:250	an essential and highly conserved post-translational modification	186:250	an essential and highly conserved post-translational modification in eukaryotes	186:264	N-Linked protein glycosylation is an essential and highly conserved post-translational modification in eukaryotes.
29042445	0	33	theme	substrate	12:20	arg1	specificity					22:32	substrate specificity	12:32	substrate specificity of Trypanosoma brucei oligosaccharyltransferases (OSTs)	12:88	Analysis of substrate specificity of Trypanosoma brucei oligosaccharyltransferases (OSTs) by functional expression of domain-swapped chimeras in yeast.
29042445	8	34	theme	amino	1636:1640	arg1	acids					1642:1646	single amino acids	1629:1646	single amino acids	1629:1646	These results provide a basis to further investigate the molecular mechanisms and contribution of single amino acids in OST interaction with its substrates.
29042445	0	35	theme	chimeras	133:140	arg1	expression					104:113	functional expression	93:113	functional expression of domain-swapped chimeras in yeast	93:149	Analysis of substrate specificity of Trypanosoma brucei oligosaccharyltransferases (OSTs) by functional expression of domain-swapped chimeras in yeast.
29042445	8	36	from	mechanisms	1598:1607	arg1	interaction					1655:1665	OST interaction	1651:1665	OST interaction with its substrates	1651:1685	These results provide a basis to further investigate the molecular mechanisms and contribution of single amino acids in OST interaction with its substrates.
29042445	1	37	theme	post-translational	220:237	arg1	glycosylation					169:181	N-Linked protein glycosylation	152:181	N-Linked protein glycosylation	152:181	N-Linked protein glycosylation is an essential and highly conserved post-translational modification in eukaryotes.
29042445	1	37	theme	post-translational	220:237	arg1	modification					239:250	an essential and highly conserved post-translational modification	186:250	an essential and highly conserved post-translational modification in eukaryotes	186:264	N-Linked protein glycosylation is an essential and highly conserved post-translational modification in eukaryotes.
29042445	0	38	theme	Trypanosoma	37:47	arg1	OSTs					84:87	OSTs	84:87	OSTs	84:87	Analysis of substrate specificity of Trypanosoma brucei oligosaccharyltransferases (OSTs) by functional expression of domain-swapped chimeras in yeast.
29042445	0	38	theme	Trypanosoma	37:47	arg1	oligosaccharyltransferases					56:81	Trypanosoma brucei oligosaccharyltransferases	37:81	Trypanosoma brucei oligosaccharyltransferases (OSTs)	37:88	Analysis of substrate specificity of Trypanosoma brucei oligosaccharyltransferases (OSTs) by functional expression of domain-swapped chimeras in yeast.
29042445	8	39	theme	single	1629:1634	arg1	acids					1642:1646	single amino acids	1629:1646	single amino acids	1629:1646	These results provide a basis to further investigate the molecular mechanisms and contribution of single amino acids in OST interaction with its substrates.
29042445	4	40	theme	genetic	945:951	arg1	tools					953:957	yeast genetic tools	939:957	yeast genetic tools	939:957	We characterized the LLO and polypeptide specificity of all three TbOST isoforms and their chimeric forms in the heterologous expression host S. cerevisiae where we were able to apply yeast genetic tools and newly developed glycoproteomics methods.
29042445	5	41	theme	LLO	1042:1044	arg1	substrates					1046:1055	LLO substrates	1042:1055	LLO substrates ranging from Man5GlcNAc2 to Man7GlcNAc2 In contrast	1042:1107	We demonstrated that TbSTT3A accepted LLO substrates ranging from Man5GlcNAc2 to Man7GlcNAc2 In contrast, TbSTT3B required more complex precursors ranging from Man6GlcNAc2 to Glc3Man9GlcNAc2 structures, and TbSTT3C did not display any LLO preference.
29042445	3	42	dep	Saccharomyces	633:645	arg1	cerevisiae					647:656	cerevisiae	647:656	cerevisiae	647:656	Trypanosoma brucei encodes three paralogue single-protein OSTs called TbSTT3A, TbSTT3B, and TbSTT3C that can functionally complement the Saccharomyces cerevisiae OST, making it an ideal experimental system to study the fundamental properties of OST activity.
29042445	2	43	theme	reticulum	480:488	arg1	lumen					455:459	the lumen	451:459	the lumen of the endoplasmic reticulum (ER)	451:493	The transfer of a glycan from a lipid-linked oligosaccharide (LLO) donor to the asparagine residue of a nascent polypeptide chain is catalyzed by an oligosaccharyltransferase (OST) in the lumen of the endoplasmic reticulum (ER).
29042445	4	44	theme	yeast	939:943	arg1	tools					953:957	yeast genetic tools	939:957	yeast genetic tools	939:957	We characterized the LLO and polypeptide specificity of all three TbOST isoforms and their chimeric forms in the heterologous expression host S. cerevisiae where we were able to apply yeast genetic tools and newly developed glycoproteomics methods.
29042445	4	45	theme	isoforms	827:834	arg1	forms					855:859	their chimeric forms	840:859	their chimeric forms in the heterologous expression host S. cerevisiae where we were able to apply yeast genetic tools and newly developed glycoproteomics methods	840:1001	We characterized the LLO and polypeptide specificity of all three TbOST isoforms and their chimeric forms in the heterologous expression host S. cerevisiae where we were able to apply yeast genetic tools and newly developed glycoproteomics methods.
29042445	4	45	theme	isoforms	827:834	arg1	specificity					796:806	the LLO and polypeptide specificity	772:806	specificity	796:806	We characterized the LLO and polypeptide specificity of all three TbOST isoforms and their chimeric forms in the heterologous expression host S. cerevisiae where we were able to apply yeast genetic tools and newly developed glycoproteomics methods.
29042445	1	46	from	modification	239:250	arg1	eukaryotes					255:264	eukaryotes	255:264	eukaryotes	255:264	N-Linked protein glycosylation is an essential and highly conserved post-translational modification in eukaryotes.
29042445	0	47	theme	oligosaccharyltransferases	56:81	arg1	specificity					22:32	substrate specificity	12:32	substrate specificity of Trypanosoma brucei oligosaccharyltransferases (OSTs)	12:88	Analysis of substrate specificity of Trypanosoma brucei oligosaccharyltransferases (OSTs) by functional expression of domain-swapped chimeras in yeast.
29042445	8	48	theme	acids	1642:1646	arg1	contribution					1613:1624	contribution	1613:1624	contribution of single amino acids in OST interaction with its substrates	1613:1685	These results provide a basis to further investigate the molecular mechanisms and contribution of single amino acids in OST interaction with its substrates.
29042445	8	48	theme	acids	1642:1646	arg1	mechanisms					1598:1607	the molecular mechanisms	1584:1607	the molecular mechanisms	1584:1607	These results provide a basis to further investigate the molecular mechanisms and contribution of single amino acids in OST interaction with its substrates.
29042445	4	49	theme	glycoproteomics	979:993	arg1	methods					995:1001	newly developed glycoproteomics methods	963:1001	newly developed glycoproteomics methods	963:1001	We characterized the LLO and polypeptide specificity of all three TbOST isoforms and their chimeric forms in the heterologous expression host S. cerevisiae where we were able to apply yeast genetic tools and newly developed glycoproteomics methods.
29042445	4	50	theme	chimeric	846:853	arg1	forms					855:859	their chimeric forms	840:859	their chimeric forms in the heterologous expression host S. cerevisiae where we were able to apply yeast genetic tools and newly developed glycoproteomics methods	840:1001	We characterized the LLO and polypeptide specificity of all three TbOST isoforms and their chimeric forms in the heterologous expression host S. cerevisiae where we were able to apply yeast genetic tools and newly developed glycoproteomics methods.
29042445	0	51	theme	brucei	49:54	arg1	OSTs					84:87	OSTs	84:87	OSTs	84:87	Analysis of substrate specificity of Trypanosoma brucei oligosaccharyltransferases (OSTs) by functional expression of domain-swapped chimeras in yeast.
29042445	0	51	theme	brucei	49:54	arg1	oligosaccharyltransferases					56:81	Trypanosoma brucei oligosaccharyltransferases	37:81	Trypanosoma brucei oligosaccharyltransferases (OSTs)	37:88	Analysis of substrate specificity of Trypanosoma brucei oligosaccharyltransferases (OSTs) by functional expression of domain-swapped chimeras in yeast.
29042445	3	52	theme	experimental	682:693	arg1	system					695:700	an ideal experimental system	673:700	an ideal experimental system to study the fundamental properties of OST activity	673:752	Trypanosoma brucei encodes three paralogue single-protein OSTs called TbSTT3A, TbSTT3B, and TbSTT3C that can functionally complement the Saccharomyces cerevisiae OST, making it an ideal experimental system to study the fundamental properties of OST activity.
29042445	3	53	theme	Trypanosoma	496:506	arg1	OSTs					554:557	three paralogue single-protein OSTs	523:557	three paralogue single-protein OSTs called TbSTT3A, TbSTT3B, and TbSTT3C that can functionally complement the Saccharomyces cerevisiae OST	523:660	Trypanosoma brucei encodes three paralogue single-protein OSTs called TbSTT3A, TbSTT3B, and TbSTT3C that can functionally complement the Saccharomyces cerevisiae OST, making it an ideal experimental system to study the fundamental properties of OST activity.
29042445	3	53	theme	Trypanosoma	496:506	arg1	brucei					508:513	Trypanosoma brucei	496:513	Trypanosoma brucei	496:513	Trypanosoma brucei encodes three paralogue single-protein OSTs called TbSTT3A, TbSTT3B, and TbSTT3C that can functionally complement the Saccharomyces cerevisiae OST, making it an ideal experimental system to study the fundamental properties of OST activity.
29042445	4	54	theme	developed	969:977	arg1	methods					995:1001	newly developed glycoproteomics methods	963:1001	newly developed glycoproteomics methods	963:1001	We characterized the LLO and polypeptide specificity of all three TbOST isoforms and their chimeric forms in the heterologous expression host S. cerevisiae where we were able to apply yeast genetic tools and newly developed glycoproteomics methods.
29042445	8	55	theme	OST	1651:1653	arg1	interaction					1655:1665	OST interaction	1651:1665	OST interaction with its substrates	1651:1685	These results provide a basis to further investigate the molecular mechanisms and contribution of single amino acids in OST interaction with its substrates.
29042445	1	56	theme	N-Linked	152:159	arg1	modification					239:250	an essential and highly conserved post-translational modification	186:250	an essential and highly conserved post-translational modification in eukaryotes	186:264	N-Linked protein glycosylation is an essential and highly conserved post-translational modification in eukaryotes.
29042445	1	56	theme	N-Linked	152:159	arg1	glycosylation					169:181	N-Linked protein glycosylation	152:181	N-Linked protein glycosylation	152:181	N-Linked protein glycosylation is an essential and highly conserved post-translational modification in eukaryotes.
29042445	3	57	theme	Saccharomyces	633:645	arg1	OST					658:660	the Saccharomyces cerevisiae OST	629:660	the Saccharomyces cerevisiae OST	629:660	Trypanosoma brucei encodes three paralogue single-protein OSTs called TbSTT3A, TbSTT3B, and TbSTT3C that can functionally complement the Saccharomyces cerevisiae OST, making it an ideal experimental system to study the fundamental properties of OST activity.
29042445	2	58	theme	asparagine	347:356	arg1	residue					358:364	the asparagine residue	343:364	the asparagine residue of a nascent polypeptide chain	343:395	The transfer of a glycan from a lipid-linked oligosaccharide (LLO) donor to the asparagine residue of a nascent polypeptide chain is catalyzed by an oligosaccharyltransferase (OST) in the lumen of the endoplasmic reticulum (ER).
29042445	2	58	theme	asparagine	347:356	arg1	chain					391:395	a nascent polypeptide chain	369:395	a nascent polypeptide chain	369:395	The transfer of a glycan from a lipid-linked oligosaccharide (LLO) donor to the asparagine residue of a nascent polypeptide chain is catalyzed by an oligosaccharyltransferase (OST) in the lumen of the endoplasmic reticulum (ER).
29042445	0	59	from	expression	104:113	arg1	yeast					145:149	yeast	145:149	yeast	145:149	Analysis of substrate specificity of Trypanosoma brucei oligosaccharyltransferases (OSTs) by functional expression of domain-swapped chimeras in yeast.
29042445	2	60	theme	glycan	285:290	arg1	transfer					271:278	The transfer	267:278	The transfer of a glycan from a lipid-linked oligosaccharide (LLO) donor to the asparagine residue of a nascent polypeptide chain	267:395	The transfer of a glycan from a lipid-linked oligosaccharide (LLO) donor to the asparagine residue of a nascent polypeptide chain is catalyzed by an oligosaccharyltransferase (OST) in the lumen of the endoplasmic reticulum (ER).
29042445	1	61	theme	protein	161:167	arg1	modification					239:250	an essential and highly conserved post-translational modification	186:250	an essential and highly conserved post-translational modification in eukaryotes	186:264	N-Linked protein glycosylation is an essential and highly conserved post-translational modification in eukaryotes.
29042445	1	61	theme	protein	161:167	arg1	glycosylation					169:181	N-Linked protein glycosylation	152:181	N-Linked protein glycosylation	152:181	N-Linked protein glycosylation is an essential and highly conserved post-translational modification in eukaryotes.
29042445	8	62	with	interaction	1655:1665	arg1	substrates					1676:1685	its substrates	1672:1685	its substrates	1672:1685	These results provide a basis to further investigate the molecular mechanisms and contribution of single amino acids in OST interaction with its substrates.
29042445	3	63	theme	ideal	676:680	arg1	system					695:700	an ideal experimental system	673:700	an ideal experimental system to study the fundamental properties of OST activity	673:752	Trypanosoma brucei encodes three paralogue single-protein OSTs called TbSTT3A, TbSTT3B, and TbSTT3C that can functionally complement the Saccharomyces cerevisiae OST, making it an ideal experimental system to study the fundamental properties of OST activity.
29042445	4	64	theme	TbOST	821:825	arg1	isoforms					827:834	all three TbOST isoforms	811:834	all three TbOST isoforms	811:834	We characterized the LLO and polypeptide specificity of all three TbOST isoforms and their chimeric forms in the heterologous expression host S. cerevisiae where we were able to apply yeast genetic tools and newly developed glycoproteomics methods.
29042445	2	65	from	oligosaccharyltransferase	416:440	arg1	lumen					455:459	the lumen	451:459	the lumen of the endoplasmic reticulum (ER)	451:493	The transfer of a glycan from a lipid-linked oligosaccharide (LLO) donor to the asparagine residue of a nascent polypeptide chain is catalyzed by an oligosaccharyltransferase (OST) in the lumen of the endoplasmic reticulum (ER).
29042445	2	66	theme	chain	391:395	arg1	residue					358:364	the asparagine residue	343:364	the asparagine residue of a nascent polypeptide chain	343:395	The transfer of a glycan from a lipid-linked oligosaccharide (LLO) donor to the asparagine residue of a nascent polypeptide chain is catalyzed by an oligosaccharyltransferase (OST) in the lumen of the endoplasmic reticulum (ER).
29042445	2	66	theme	chain	391:395	arg1	chain					391:395	a nascent polypeptide chain	369:395	a nascent polypeptide chain	369:395	The transfer of a glycan from a lipid-linked oligosaccharide (LLO) donor to the asparagine residue of a nascent polypeptide chain is catalyzed by an oligosaccharyltransferase (OST) in the lumen of the endoplasmic reticulum (ER).
29042445	7	67	theme	individual	1352:1361	arg1	regions					1363:1369	the individual regions	1348:1369	the individual regions between different OST proteins	1348:1400	We have swapped the individual regions between different OST proteins and identified region 2 to influence the specificity toward the LLO and region 1 to influence polypeptide substrate specificity.
29042445	2	68	theme	endoplasmic	468:478	arg1	ER					491:492	ER	491:492	ER	491:492	The transfer of a glycan from a lipid-linked oligosaccharide (LLO) donor to the asparagine residue of a nascent polypeptide chain is catalyzed by an oligosaccharyltransferase (OST) in the lumen of the endoplasmic reticulum (ER).
29042445	2	68	theme	endoplasmic	468:478	arg1	reticulum					480:488	the endoplasmic reticulum	464:488	the endoplasmic reticulum (ER)	464:493	The transfer of a glycan from a lipid-linked oligosaccharide (LLO) donor to the asparagine residue of a nascent polypeptide chain is catalyzed by an oligosaccharyltransferase (OST) in the lumen of the endoplasmic reticulum (ER).
29042445	4	69	dep	host	892:895	arg1	cerevisiae					900:909	S. cerevisiae	897:909	the heterologous expression host S. cerevisiae where we were able to apply yeast genetic tools and newly developed glycoproteomics methods	864:1001	We characterized the LLO and polypeptide specificity of all three TbOST isoforms and their chimeric forms in the heterologous expression host S. cerevisiae where we were able to apply yeast genetic tools and newly developed glycoproteomics methods.
29042445	2	70	theme	polypeptide	379:389	arg1	chain					391:395	a nascent polypeptide chain	369:395	a nascent polypeptide chain	369:395	The transfer of a glycan from a lipid-linked oligosaccharide (LLO) donor to the asparagine residue of a nascent polypeptide chain is catalyzed by an oligosaccharyltransferase (OST) in the lumen of the endoplasmic reticulum (ER).
26123816	8	0	theme	lower	1192:1196	arg1	temperature					1198:1208	lower temperature	1192:1208	lower temperature	1192:1208	Upon addition of sodium alginate to polyvinyl alcohol and increasing silver nanoparticles, the melting peak shifted to lower temperature and crystallinity percent was decreased.
26123816	7	1	theme	SEM	1024:1026	arg1	images					1028:1033	the SEM images	1020:1033	the SEM images	1020:1033	Good miscibility of polyvinyl alcohol and sodium alginate observed on the SEM images was supported with FTIR spectroscopy.
26123816	0	2	theme	gamma	90:94	arg1	irradiation					96:106	gamma irradiation	90:106	gamma irradiation	90:106	Antibacterial silver nanoparticles in polyvinyl alcohol/sodium alginate blend produced by gamma irradiation.
26123816	6	3	theme	PVA/SA	859:864	arg1	stabilizer					882:891	an efficient stabilizer	869:891	an efficient stabilizer in preventing agglomeration of the silver nanoparticles	869:947	Comparing SEM images with DLS results indicated good performance of PVA/SA as an efficient stabilizer in preventing agglomeration of the silver nanoparticles.
26123816	6	3	theme	PVA/SA	859:864	arg1	performance					844:854	good performance	839:854	good performance of PVA/SA	839:864	Comparing SEM images with DLS results indicated good performance of PVA/SA as an efficient stabilizer in preventing agglomeration of the silver nanoparticles.
26123816	10	4	theme	nanoparticles	1465:1477	arg1	level					1449:1453	very low level	1440:1453	very low level of silver nanoparticles	1440:1477	The composites exhibited strong antibacterial activity against Staphylococcus aureus and Escherichia coli even at very low level of silver nanoparticles.
26123816	9	5	from	increase	1297:1304	arg1	rigidity					1309:1316	rigidity	1309:1316	rigidity of PVA	1309:1323	Addition of sodium alginate led to remarkable increase in rigidity of PVA.
26123816	4	6	theme	more	547:550	arg1	nanoparticles					552:564	more nanoparticles	547:564	more nanoparticles	547:564	By increasing irradiation dose, more nanoparticles were synthesized while no PVA hydrogel was formed up to 15 kGy.
26123816	5	7	theme	gamma	689:693	arg1	dose					695:698	gamma dose	689:698	gamma dose evidenced by higher release rate of silver nanoparticles	689:755	Also the size of nanoparticles was reduced with increasing gamma dose evidenced by higher release rate of silver nanoparticles in lukewarm water and SEM images.
26123816	6	8	theme	good	839:842	arg1	stabilizer					882:891	an efficient stabilizer	869:891	an efficient stabilizer in preventing agglomeration of the silver nanoparticles	869:947	Comparing SEM images with DLS results indicated good performance of PVA/SA as an efficient stabilizer in preventing agglomeration of the silver nanoparticles.
26123816	6	8	theme	good	839:842	arg1	performance					844:854	good performance	839:854	good performance of PVA/SA	839:864	Comparing SEM images with DLS results indicated good performance of PVA/SA as an efficient stabilizer in preventing agglomeration of the silver nanoparticles.
26123816	2	9	theme	nitrate	315:321	arg1	reduction					295:303	reduction	295:303	reduction of silver nitrate	295:321	Gamma irradiation was used to synthesize silver nanoparticles in situ via reduction of silver nitrate without using harmful chemical agents for biomedical applications.
26123816	6	10	dep	indicated	829:837	arg1	Comparing					791:799	Comparing	791:799	Comparing SEM images with DLS	791:819	Comparing SEM images with DLS results indicated good performance of PVA/SA as an efficient stabilizer in preventing agglomeration of the silver nanoparticles.
26123816	3	11	theme	XRD	401:403	arg1	results					405:411	UV-vis and XRD results	390:411	UV-vis and XRD results	390:411	UV-vis and XRD results demonstrated that spherical silver nanoparticles were produced even at low irradiation dose of 5 kGy.
26123816	6	12	theme	silver	928:933	arg1	nanoparticles					935:947	the silver nanoparticles	924:947	the silver nanoparticles	924:947	Comparing SEM images with DLS results indicated good performance of PVA/SA as an efficient stabilizer in preventing agglomeration of the silver nanoparticles.
26123816	7	13	theme	sodium	992:997	arg1	alginate					999:1006	sodium alginate	992:1006	sodium alginate	992:1006	Good miscibility of polyvinyl alcohol and sodium alginate observed on the SEM images was supported with FTIR spectroscopy.
26123816	2	14	theme	silver	308:313	arg1	nitrate					315:321	silver nitrate	308:321	silver nitrate	308:321	Gamma irradiation was used to synthesize silver nanoparticles in situ via reduction of silver nitrate without using harmful chemical agents for biomedical applications.
26123816	10	15	theme	strong	1351:1356	arg1	activity					1372:1379	strong antibacterial activity	1351:1379	strong antibacterial activity against Staphylococcus aureus and Escherichia coli	1351:1430	The composites exhibited strong antibacterial activity against Staphylococcus aureus and Escherichia coli even at very low level of silver nanoparticles.
26123816	8	16	theme	alginate	1097:1104	arg1	addition					1078:1085	addition	1078:1085	addition of sodium alginate to polyvinyl alcohol and increasing silver nanoparticles	1078:1161	Upon addition of sodium alginate to polyvinyl alcohol and increasing silver nanoparticles, the melting peak shifted to lower temperature and crystallinity percent was decreased.
26123816	7	17	theme	alginate	999:1006	arg1	miscibility					955:965	Good miscibility	950:965	Good miscibility of polyvinyl alcohol and sodium alginate observed on the SEM images	950:1033	Good miscibility of polyvinyl alcohol and sodium alginate observed on the SEM images was supported with FTIR spectroscopy.
26123816	3	18	theme	low	484:486	arg1	dose					500:503	low irradiation dose	484:503	low irradiation dose of 5 kGy	484:512	UV-vis and XRD results demonstrated that spherical silver nanoparticles were produced even at low irradiation dose of 5 kGy.
26123816	3	19	theme	UV-vis	390:395	arg1	results					405:411	UV-vis and XRD results	390:411	UV-vis and XRD results	390:411	UV-vis and XRD results demonstrated that spherical silver nanoparticles were produced even at low irradiation dose of 5 kGy.
26123816	4	20	theme	irradiation	529:539	arg1	dose					541:544	irradiation dose	529:544	irradiation dose	529:544	By increasing irradiation dose, more nanoparticles were synthesized while no PVA hydrogel was formed up to 15 kGy.
26123816	5	21	theme	nanoparticles	647:659	arg1	size					639:642	the size	635:642	the size of nanoparticles	635:659	Also the size of nanoparticles was reduced with increasing gamma dose evidenced by higher release rate of silver nanoparticles in lukewarm water and SEM images.
26123816	7	22	theme	alcohol	980:986	arg1	miscibility					955:965	Good miscibility	950:965	Good miscibility of polyvinyl alcohol and sodium alginate observed on the SEM images	950:1033	Good miscibility of polyvinyl alcohol and sodium alginate observed on the SEM images was supported with FTIR spectroscopy.
26123816	3	23	theme	spherical	431:439	arg1	nanoparticles					448:460	spherical silver nanoparticles	431:460	spherical silver nanoparticles	431:460	UV-vis and XRD results demonstrated that spherical silver nanoparticles were produced even at low irradiation dose of 5 kGy.
26123816	8	24	theme	silver	1142:1147	arg1	nanoparticles					1149:1161	increasing silver nanoparticles	1131:1161	increasing silver nanoparticles	1131:1161	Upon addition of sodium alginate to polyvinyl alcohol and increasing silver nanoparticles, the melting peak shifted to lower temperature and crystallinity percent was decreased.
26123816	1	25	theme	solution	196:203	arg1	method					213:218	solution casting method	196:218	solution casting method	196:218	Polyvinyl alcohol/sodium alginate/nano silver (PVA/SA/Ag) composite films were made by solution casting method.
26123816	8	26	theme	polyvinyl	1109:1117	arg1	alcohol					1119:1125	polyvinyl alcohol	1109:1125	polyvinyl alcohol	1109:1125	Upon addition of sodium alginate to polyvinyl alcohol and increasing silver nanoparticles, the melting peak shifted to lower temperature and crystallinity percent was decreased.
26123816	9	27	theme	sodium	1263:1268	arg1	alginate					1270:1277	sodium alginate	1263:1277	sodium alginate	1263:1277	Addition of sodium alginate led to remarkable increase in rigidity of PVA.
26123816	2	28	theme	chemical	345:352	arg1	agents					354:359	harmful chemical agents	337:359	harmful chemical agents for biomedical applications	337:387	Gamma irradiation was used to synthesize silver nanoparticles in situ via reduction of silver nitrate without using harmful chemical agents for biomedical applications.
26123816	1	29	theme	casting	205:211	arg1	method					213:218	solution casting method	196:218	solution casting method	196:218	Polyvinyl alcohol/sodium alginate/nano silver (PVA/SA/Ag) composite films were made by solution casting method.
26123816	0	30	theme	Antibacterial	0:12	arg1	silver					14:19	Antibacterial silver	0:19	Antibacterial silver	0:19	Antibacterial silver nanoparticles in polyvinyl alcohol/sodium alginate blend produced by gamma irradiation.
26123816	9	31	theme	alginate	1270:1277	arg1	Addition					1251:1258	Addition	1251:1258	Addition of sodium alginate	1251:1277	Addition of sodium alginate led to remarkable increase in rigidity of PVA.
26123816	1	32	theme	Polyvinyl	109:117	arg1	PVA/SA/Ag					156:164	PVA/SA/Ag	156:164	PVA/SA/Ag	156:164	Polyvinyl alcohol/sodium alginate/nano silver (PVA/SA/Ag) composite films were made by solution casting method.
26123816	1	32	theme	Polyvinyl	109:117	arg1	silver					148:153	Polyvinyl alcohol/sodium alginate/nano silver	109:153	Polyvinyl alcohol/sodium alginate/nano silver (PVA/SA/Ag) composite films	109:181	Polyvinyl alcohol/sodium alginate/nano silver (PVA/SA/Ag) composite films were made by solution casting method.
26123816	2	33	used	used	243:246	arg2	irradiation					227:237	Gamma irradiation	221:237	Gamma irradiation	221:237	Gamma irradiation was used to synthesize silver nanoparticles in situ via reduction of silver nitrate without using harmful chemical agents for biomedical applications.
26123816	4	34	theme	PVA	592:594	arg1	hydrogel					596:603	no PVA hydrogel	589:603	no PVA hydrogel	589:603	By increasing irradiation dose, more nanoparticles were synthesized while no PVA hydrogel was formed up to 15 kGy.
26123816	6	35	theme	SEM	801:803	arg1	images					805:810	SEM images	801:810	SEM images	801:810	Comparing SEM images with DLS results indicated good performance of PVA/SA as an efficient stabilizer in preventing agglomeration of the silver nanoparticles.
26123816	1	36	theme	alcohol/sodium	119:132	arg1	PVA/SA/Ag					156:164	PVA/SA/Ag	156:164	PVA/SA/Ag	156:164	Polyvinyl alcohol/sodium alginate/nano silver (PVA/SA/Ag) composite films were made by solution casting method.
26123816	1	36	theme	alcohol/sodium	119:132	arg1	silver					148:153	Polyvinyl alcohol/sodium alginate/nano silver	109:153	Polyvinyl alcohol/sodium alginate/nano silver (PVA/SA/Ag) composite films	109:181	Polyvinyl alcohol/sodium alginate/nano silver (PVA/SA/Ag) composite films were made by solution casting method.
26123816	7	37	located	observed	1008:1015	arg1	images					1028:1033	the SEM images	1020:1033	the SEM images	1020:1033	Good miscibility of polyvinyl alcohol and sodium alginate observed on the SEM images was supported with FTIR spectroscopy.
26123816	7	37	located	observed	1008:1015	arg2	alcohol					980:986	polyvinyl alcohol	970:986	polyvinyl alcohol	970:986	Good miscibility of polyvinyl alcohol and sodium alginate observed on the SEM images was supported with FTIR spectroscopy.
26123816	2	38	theme	harmful	337:343	arg1	agents					354:359	harmful chemical agents	337:359	harmful chemical agents for biomedical applications	337:387	Gamma irradiation was used to synthesize silver nanoparticles in situ via reduction of silver nitrate without using harmful chemical agents for biomedical applications.
26123816	9	39	theme	PVA	1321:1323	arg1	rigidity					1309:1316	rigidity	1309:1316	rigidity of PVA	1309:1323	Addition of sodium alginate led to remarkable increase in rigidity of PVA.
26123816	4	40	dep	kGy	625:627	arg1	up					616:617	up	616:617	up	616:617	By increasing irradiation dose, more nanoparticles were synthesized while no PVA hydrogel was formed up to 15 kGy.
26123816	1	41	theme	alginate/nano	134:146	arg1	PVA/SA/Ag					156:164	PVA/SA/Ag	156:164	PVA/SA/Ag	156:164	Polyvinyl alcohol/sodium alginate/nano silver (PVA/SA/Ag) composite films were made by solution casting method.
26123816	1	41	theme	alginate/nano	134:146	arg1	silver					148:153	Polyvinyl alcohol/sodium alginate/nano silver	109:153	Polyvinyl alcohol/sodium alginate/nano silver (PVA/SA/Ag) composite films	109:181	Polyvinyl alcohol/sodium alginate/nano silver (PVA/SA/Ag) composite films were made by solution casting method.
26123816	3	42	theme	kGy	510:512	arg1	dose					500:503	low irradiation dose	484:503	low irradiation dose of 5 kGy	484:512	UV-vis and XRD results demonstrated that spherical silver nanoparticles were produced even at low irradiation dose of 5 kGy.
26123816	0	43	theme	alcohol/sodium	48:61	arg1	blend					72:76	polyvinyl alcohol/sodium alginate blend	38:76	polyvinyl alcohol/sodium alginate blend produced by gamma irradiation	38:106	Antibacterial silver nanoparticles in polyvinyl alcohol/sodium alginate blend produced by gamma irradiation.
26123816	5	44	theme	silver	736:741	arg1	nanoparticles					743:755	silver nanoparticles	736:755	silver nanoparticles	736:755	Also the size of nanoparticles was reduced with increasing gamma dose evidenced by higher release rate of silver nanoparticles in lukewarm water and SEM images.
26123816	9	45	theme	remarkable	1286:1295	arg1	increase					1297:1304	remarkable increase	1286:1304	remarkable increase in rigidity of PVA	1286:1323	Addition of sodium alginate led to remarkable increase in rigidity of PVA.
26123816	2	46	theme	silver	262:267	arg1	nanoparticles					269:281	silver nanoparticles	262:281	silver nanoparticles	262:281	Gamma irradiation was used to synthesize silver nanoparticles in situ via reduction of silver nitrate without using harmful chemical agents for biomedical applications.
26123816	10	47	theme	antibacterial	1358:1370	arg1	activity					1372:1379	strong antibacterial activity	1351:1379	strong antibacterial activity against Staphylococcus aureus and Escherichia coli	1351:1430	The composites exhibited strong antibacterial activity against Staphylococcus aureus and Escherichia coli even at very low level of silver nanoparticles.
26123816	0	48	theme	polyvinyl	38:46	arg1	blend					72:76	polyvinyl alcohol/sodium alginate blend	38:76	polyvinyl alcohol/sodium alginate blend produced by gamma irradiation	38:106	Antibacterial silver nanoparticles in polyvinyl alcohol/sodium alginate blend produced by gamma irradiation.
26123816	5	49	theme	nanoparticles	743:755	arg1	rate					728:731	higher release rate	713:731	higher release rate of silver nanoparticles	713:755	Also the size of nanoparticles was reduced with increasing gamma dose evidenced by higher release rate of silver nanoparticles in lukewarm water and SEM images.
26123816	7	50	theme	FTIR	1054:1057	arg1	spectroscopy					1059:1070	FTIR spectroscopy	1054:1070	FTIR spectroscopy	1054:1070	Good miscibility of polyvinyl alcohol and sodium alginate observed on the SEM images was supported with FTIR spectroscopy.
26123816	8	51	theme	crystallinity	1214:1226	arg1	percent					1228:1234	crystallinity percent	1214:1234	crystallinity percent	1214:1234	Upon addition of sodium alginate to polyvinyl alcohol and increasing silver nanoparticles, the melting peak shifted to lower temperature and crystallinity percent was decreased.
26123816	3	52	theme	silver	441:446	arg1	nanoparticles					448:460	spherical silver nanoparticles	431:460	spherical silver nanoparticles	431:460	UV-vis and XRD results demonstrated that spherical silver nanoparticles were produced even at low irradiation dose of 5 kGy.
26123816	5	53	dep	water	769:773	arg1	images					783:788	images	783:788	images	783:788	Also the size of nanoparticles was reduced with increasing gamma dose evidenced by higher release rate of silver nanoparticles in lukewarm water and SEM images.
26123816	6	54	theme	efficient	872:880	arg1	stabilizer					882:891	an efficient stabilizer	869:891	an efficient stabilizer in preventing agglomeration of the silver nanoparticles	869:947	Comparing SEM images with DLS results indicated good performance of PVA/SA as an efficient stabilizer in preventing agglomeration of the silver nanoparticles.
26123816	6	54	theme	efficient	872:880	arg1	performance					844:854	good performance	839:854	good performance of PVA/SA	839:864	Comparing SEM images with DLS results indicated good performance of PVA/SA as an efficient stabilizer in preventing agglomeration of the silver nanoparticles.
26123816	8	55	theme	melting	1168:1174	arg1	peak					1176:1179	the melting peak	1164:1179	the melting peak shifted to lower temperature and crystallinity percent	1164:1234	Upon addition of sodium alginate to polyvinyl alcohol and increasing silver nanoparticles, the melting peak shifted to lower temperature and crystallinity percent was decreased.
26123816	1	56	theme	silver	148:153	arg1	films					177:181	Polyvinyl alcohol/sodium alginate/nano silver (PVA/SA/Ag) composite films	109:181	Polyvinyl alcohol/sodium alginate/nano silver (PVA/SA/Ag) composite films	109:181	Polyvinyl alcohol/sodium alginate/nano silver (PVA/SA/Ag) composite films were made by solution casting method.
26123816	0	57	theme	alginate	63:70	arg1	blend					72:76	polyvinyl alcohol/sodium alginate blend	38:76	polyvinyl alcohol/sodium alginate blend produced by gamma irradiation	38:106	Antibacterial silver nanoparticles in polyvinyl alcohol/sodium alginate blend produced by gamma irradiation.
26123816	8	58	theme	sodium	1090:1095	arg1	alginate					1097:1104	sodium alginate	1090:1104	sodium alginate	1090:1104	Upon addition of sodium alginate to polyvinyl alcohol and increasing silver nanoparticles, the melting peak shifted to lower temperature and crystallinity percent was decreased.
26123816	2	59	theme	biomedical	365:374	arg1	applications					376:387	biomedical applications	365:387	biomedical applications	365:387	Gamma irradiation was used to synthesize silver nanoparticles in situ via reduction of silver nitrate without using harmful chemical agents for biomedical applications.
26123816	7	60	theme	Good	950:953	arg1	miscibility					955:965	Good miscibility	950:965	Good miscibility of polyvinyl alcohol and sodium alginate observed on the SEM images	950:1033	Good miscibility of polyvinyl alcohol and sodium alginate observed on the SEM images was supported with FTIR spectroscopy.
26123816	7	61	theme	polyvinyl	970:978	arg1	alcohol					980:986	polyvinyl alcohol	970:986	polyvinyl alcohol	970:986	Good miscibility of polyvinyl alcohol and sodium alginate observed on the SEM images was supported with FTIR spectroscopy.
26123816	5	62	theme	higher	713:718	arg1	rate					728:731	higher release rate	713:731	higher release rate of silver nanoparticles	713:755	Also the size of nanoparticles was reduced with increasing gamma dose evidenced by higher release rate of silver nanoparticles in lukewarm water and SEM images.
26123816	10	63	theme	silver	1458:1463	arg1	nanoparticles					1465:1477	silver nanoparticles	1458:1477	silver nanoparticles	1458:1477	The composites exhibited strong antibacterial activity against Staphylococcus aureus and Escherichia coli even at very low level of silver nanoparticles.
26123816	5	64	theme	release	720:726	arg1	rate					728:731	higher release rate	713:731	higher release rate of silver nanoparticles	713:755	Also the size of nanoparticles was reduced with increasing gamma dose evidenced by higher release rate of silver nanoparticles in lukewarm water and SEM images.
26123816	2	65	theme	Gamma	221:225	arg1	irradiation					227:237	Gamma irradiation	221:237	Gamma irradiation	221:237	Gamma irradiation was used to synthesize silver nanoparticles in situ via reduction of silver nitrate without using harmful chemical agents for biomedical applications.
26123816	10	66	theme	low	1445:1447	arg1	level					1449:1453	very low level	1440:1453	very low level of silver nanoparticles	1440:1477	The composites exhibited strong antibacterial activity against Staphylococcus aureus and Escherichia coli even at very low level of silver nanoparticles.
26123816	1	67	theme	composite	167:175	arg1	films					177:181	Polyvinyl alcohol/sodium alginate/nano silver (PVA/SA/Ag) composite films	109:181	Polyvinyl alcohol/sodium alginate/nano silver (PVA/SA/Ag) composite films	109:181	Polyvinyl alcohol/sodium alginate/nano silver (PVA/SA/Ag) composite films were made by solution casting method.
26123816	3	68	theme	irradiation	488:498	arg1	dose					500:503	low irradiation dose	484:503	low irradiation dose of 5 kGy	484:512	UV-vis and XRD results demonstrated that spherical silver nanoparticles were produced even at low irradiation dose of 5 kGy.
26123816	6	69	theme	nanoparticles	935:947	arg1	agglomeration					907:919	agglomeration	907:919	agglomeration of the silver nanoparticles	907:947	Comparing SEM images with DLS results indicated good performance of PVA/SA as an efficient stabilizer in preventing agglomeration of the silver nanoparticles.
26123816	8	70	theme	increasing	1131:1140	arg1	nanoparticles					1149:1161	increasing silver nanoparticles	1131:1161	increasing silver nanoparticles	1131:1161	Upon addition of sodium alginate to polyvinyl alcohol and increasing silver nanoparticles, the melting peak shifted to lower temperature and crystallinity percent was decreased.
26123816	5	71	theme	lukewarm	760:767	arg1	water					769:773	lukewarm water	760:773	lukewarm water	760:773	Also the size of nanoparticles was reduced with increasing gamma dose evidenced by higher release rate of silver nanoparticles in lukewarm water and SEM images.
27987892	0	0	theme	transport	58:66	arg1	properties					68:77	the transport properties	54:77	the transport properties of Chitosan-Pectin membranes	54:106	Influence of Pectin as a green polymer electrolyte on the transport properties of Chitosan-Pectin membranes.
27987892	4	1	theme	ionic	486:490	arg1	pairs					492:496	the ionic pairs	482:496	the ionic pairs	482:496	DSC analysis reveals an endothermic peak due to the scission of the ionic pairs between carboxylic groups and ammonium groups, which produces a strong change on physical properties such as methanol permeability and proton conductivity.
27987892	0	2	theme	Chitosan-Pectin	82:96	arg1	membranes					98:106	Chitosan-Pectin membranes	82:106	Chitosan-Pectin membranes	82:106	Influence of Pectin as a green polymer electrolyte on the transport properties of Chitosan-Pectin membranes.
27987892	4	3	theme	strong	562:567	arg1	change					569:574	a strong change	560:574	a strong change on physical properties such as methanol permeability and proton conductivity	560:651	DSC analysis reveals an endothermic peak due to the scission of the ionic pairs between carboxylic groups and ammonium groups, which produces a strong change on physical properties such as methanol permeability and proton conductivity.
27987892	0	4	from	electrolyte	39:49	arg1	properties					68:77	the transport properties	54:77	the transport properties of Chitosan-Pectin membranes	54:106	Influence of Pectin as a green polymer electrolyte on the transport properties of Chitosan-Pectin membranes.
27987892	6	5	theme	similar	920:926	arg1	behavior					928:935	a similar behavior	918:935	a similar behavior	918:935	The proton conductivities of the blend membranes follow a similar behavior.
27987892	5	6	theme	pure	747:750	arg1	membrane					761:768	pure Chitosan membrane	747:768	pure Chitosan membrane	747:768	The methanol permeability decreases with the amount of Pectin from (4.24±0.04)×10-6cm2/s for pure Chitosan membrane to (1.51±0.03)×10-6cm2/s for blend CH/PEC membranes when the amount of Pectin is 50% (v/v).
27987892	7	7	from	drops	1088:1092	arg1	v/v					1056:1058	v/v	1056:1058	v/v	1056:1058	For a pure CH membrane the conductivity is 2.44×10-3S/cm, decreasing with pectin content until the composition 50/50 (v/v), in which the conductivity drops almost one order of magnitude.
27987892	7	7	from	drops	1088:1092	arg1	50/50					1049:1053	the composition 50/50	1033:1053	the composition 50/50 (v/v)	1033:1059	For a pure CH membrane the conductivity is 2.44×10-3S/cm, decreasing with pectin content until the composition 50/50 (v/v), in which the conductivity drops almost one order of magnitude.
27987892	4	8	theme	proton	633:638	arg1	conductivity					640:651	proton conductivity	633:651	proton conductivity	633:651	DSC analysis reveals an endothermic peak due to the scission of the ionic pairs between carboxylic groups and ammonium groups, which produces a strong change on physical properties such as methanol permeability and proton conductivity.
27987892	0	9	theme	membranes	98:106	arg1	properties					68:77	the transport properties	54:77	the transport properties of Chitosan-Pectin membranes	54:106	Influence of Pectin as a green polymer electrolyte on the transport properties of Chitosan-Pectin membranes.
27987892	5	10	theme	Chitosan	752:759	arg1	membrane					761:768	pure Chitosan membrane	747:768	pure Chitosan membrane	747:768	The methanol permeability decreases with the amount of Pectin from (4.24±0.04)×10-6cm2/s for pure Chitosan membrane to (1.51±0.03)×10-6cm2/s for blend CH/PEC membranes when the amount of Pectin is 50% (v/v).
27987892	7	11	theme	magnitude	1114:1122	arg1	order					1105:1109	almost one order	1094:1109	almost one order of magnitude	1094:1122	For a pure CH membrane the conductivity is 2.44×10-3S/cm, decreasing with pectin content until the composition 50/50 (v/v), in which the conductivity drops almost one order of magnitude.
27987892	4	12	from	change	569:574	arg1	permeability					616:627	methanol permeability	607:627	methanol permeability	607:627	DSC analysis reveals an endothermic peak due to the scission of the ionic pairs between carboxylic groups and ammonium groups, which produces a strong change on physical properties such as methanol permeability and proton conductivity.
27987892	4	12	from	change	569:574	arg1	conductivity					640:651	proton conductivity	633:651	proton conductivity	633:651	DSC analysis reveals an endothermic peak due to the scission of the ionic pairs between carboxylic groups and ammonium groups, which produces a strong change on physical properties such as methanol permeability and proton conductivity.
27987892	4	12	from	change	569:574	arg1	properties					588:597	physical properties	579:597	physical properties such as methanol permeability and proton conductivity	579:651	DSC analysis reveals an endothermic peak due to the scission of the ionic pairs between carboxylic groups and ammonium groups, which produces a strong change on physical properties such as methanol permeability and proton conductivity.
27987892	5	13	theme	methanol	658:665	arg1	permeability					667:678	The methanol permeability	654:678	The methanol permeability	654:678	The methanol permeability decreases with the amount of Pectin from (4.24±0.04)×10-6cm2/s for pure Chitosan membrane to (1.51±0.03)×10-6cm2/s for blend CH/PEC membranes when the amount of Pectin is 50% (v/v).
27987892	3	14	theme	physical	368:375	arg1	properties					377:386	the chemical and physical properties	351:386	the chemical and physical properties as a function of composition	351:415	The results show that CH/PEC membranes display structural changes on the chemical and physical properties as a function of composition.
27987892	4	15	theme	ammonium	528:535	arg1	groups					537:542	ammonium groups	528:542	ammonium groups	528:542	DSC analysis reveals an endothermic peak due to the scission of the ionic pairs between carboxylic groups and ammonium groups, which produces a strong change on physical properties such as methanol permeability and proton conductivity.
27987892	6	16	theme	proton	866:871	arg1	conductivities					873:886	The proton conductivities	862:886	The proton conductivities of the blend membranes	862:909	The proton conductivities of the blend membranes follow a similar behavior.
27987892	5	17	theme	Pectin	709:714	arg1	Pectin					709:714	Pectin	709:714	Pectin	709:714	The methanol permeability decreases with the amount of Pectin from (4.24±0.04)×10-6cm2/s for pure Chitosan membrane to (1.51±0.03)×10-6cm2/s for blend CH/PEC membranes when the amount of Pectin is 50% (v/v).
27987892	5	17	theme	Pectin	709:714	arg1	amount					699:704	the amount	695:704	the amount of Pectin from (4.24±0.04)×10-6cm2/s for pure Chitosan membrane to (1.51±0.03)×10-6cm2/s for blend CH/PEC membranes	695:820	The methanol permeability decreases with the amount of Pectin from (4.24±0.04)×10-6cm2/s for pure Chitosan membrane to (1.51±0.03)×10-6cm2/s for blend CH/PEC membranes when the amount of Pectin is 50% (v/v).
27987892	3	18	theme	composition	405:415	arg1	function					393:400	a function	391:400	a function of composition	391:415	The results show that CH/PEC membranes display structural changes on the chemical and physical properties as a function of composition.
27987892	5	19	theme	blend	799:803	arg1	membranes					812:820	blend CH/PEC membranes	799:820	blend CH/PEC membranes	799:820	The methanol permeability decreases with the amount of Pectin from (4.24±0.04)×10-6cm2/s for pure Chitosan membrane to (1.51±0.03)×10-6cm2/s for blend CH/PEC membranes when the amount of Pectin is 50% (v/v).
27987892	3	20	theme	chemical	355:362	arg1	properties					377:386	the chemical and physical properties	351:386	the chemical and physical properties as a function of composition	351:415	The results show that CH/PEC membranes display structural changes on the chemical and physical properties as a function of composition.
27987892	6	21	theme	membranes	901:909	arg1	conductivities					873:886	The proton conductivities	862:886	The proton conductivities of the blend membranes	862:909	The proton conductivities of the blend membranes follow a similar behavior.
27987892	7	22	theme	pectin	1012:1017	arg1	content					1019:1025	pectin content	1012:1025	pectin content	1012:1025	For a pure CH membrane the conductivity is 2.44×10-3S/cm, decreasing with pectin content until the composition 50/50 (v/v), in which the conductivity drops almost one order of magnitude.
27987892	5	23	theme	CH/PEC	805:810	arg1	membranes					812:820	blend CH/PEC membranes	799:820	blend CH/PEC membranes	799:820	The methanol permeability decreases with the amount of Pectin from (4.24±0.04)×10-6cm2/s for pure Chitosan membrane to (1.51±0.03)×10-6cm2/s for blend CH/PEC membranes when the amount of Pectin is 50% (v/v).
27987892	0	24	theme	green	25:29	arg1	electrolyte					39:49	a green polymer electrolyte	23:49	a green polymer electrolyte on the transport properties of Chitosan-Pectin membranes	23:106	Influence of Pectin as a green polymer electrolyte on the transport properties of Chitosan-Pectin membranes.
27987892	6	25	theme	blend	895:899	arg1	membranes					901:909	the blend membranes	891:909	the blend membranes	891:909	The proton conductivities of the blend membranes follow a similar behavior.
27987892	5	26	theme	Pectin	841:846	arg1	%					853:853	50%	851:853	50% (v/v)	851:859	The methanol permeability decreases with the amount of Pectin from (4.24±0.04)×10-6cm2/s for pure Chitosan membrane to (1.51±0.03)×10-6cm2/s for blend CH/PEC membranes when the amount of Pectin is 50% (v/v).
27987892	5	26	theme	Pectin	841:846	arg1	amount					831:836	the amount	827:836	the amount of Pectin	827:846	The methanol permeability decreases with the amount of Pectin from (4.24±0.04)×10-6cm2/s for pure Chitosan membrane to (1.51±0.03)×10-6cm2/s for blend CH/PEC membranes when the amount of Pectin is 50% (v/v).
27987892	5	26	theme	Pectin	841:846	arg1	Pectin					841:846	Pectin	841:846	Pectin	841:846	The methanol permeability decreases with the amount of Pectin from (4.24±0.04)×10-6cm2/s for pure Chitosan membrane to (1.51±0.03)×10-6cm2/s for blend CH/PEC membranes when the amount of Pectin is 50% (v/v).
27987892	4	27	theme	endothermic	442:452	arg1	peak					454:457	an endothermic peak	439:457	an endothermic peak	439:457	DSC analysis reveals an endothermic peak due to the scission of the ionic pairs between carboxylic groups and ammonium groups, which produces a strong change on physical properties such as methanol permeability and proton conductivity.
27987892	7	28	theme	CH	949:950	arg1	membrane					952:959	a pure CH membrane	942:959	a pure CH membrane	942:959	For a pure CH membrane the conductivity is 2.44×10-3S/cm, decreasing with pectin content until the composition 50/50 (v/v), in which the conductivity drops almost one order of magnitude.
27987892	4	29	theme	methanol	607:614	arg1	permeability					616:627	methanol permeability	607:627	methanol permeability	607:627	DSC analysis reveals an endothermic peak due to the scission of the ionic pairs between carboxylic groups and ammonium groups, which produces a strong change on physical properties such as methanol permeability and proton conductivity.
27987892	4	30	theme	pairs	492:496	arg1	scission					470:477	the scission	466:477	the scission	466:477	DSC analysis reveals an endothermic peak due to the scission of the ionic pairs between carboxylic groups and ammonium groups, which produces a strong change on physical properties such as methanol permeability and proton conductivity.
27987892	1	31	theme	Novel	109:113	arg1	membranes					121:129	Novel blend membranes	109:129	Novel blend membranes	109:129	Novel blend membranes have been prepared from Chitosan (CH), Pectin (PEC) and their mixtures.
27987892	0	32	theme	polymer	31:37	arg1	electrolyte					39:49	a green polymer electrolyte	23:49	a green polymer electrolyte on the transport properties of Chitosan-Pectin membranes	23:106	Influence of Pectin as a green polymer electrolyte on the transport properties of Chitosan-Pectin membranes.
27987892	3	33	theme	structural	329:338	arg1	changes					340:346	structural changes	329:346	structural changes	329:346	The results show that CH/PEC membranes display structural changes on the chemical and physical properties as a function of composition.
27987892	7	34	theme	composition	1037:1047	arg1	v/v					1056:1058	v/v	1056:1058	v/v	1056:1058	For a pure CH membrane the conductivity is 2.44×10-3S/cm, decreasing with pectin content until the composition 50/50 (v/v), in which the conductivity drops almost one order of magnitude.
27987892	7	34	theme	composition	1037:1047	arg1	50/50					1049:1053	the composition 50/50	1033:1053	the composition 50/50 (v/v)	1033:1059	For a pure CH membrane the conductivity is 2.44×10-3S/cm, decreasing with pectin content until the composition 50/50 (v/v), in which the conductivity drops almost one order of magnitude.
27987892	1	35	theme	blend	115:119	arg1	membranes					121:129	Novel blend membranes	109:129	Novel blend membranes	109:129	Novel blend membranes have been prepared from Chitosan (CH), Pectin (PEC) and their mixtures.
27987892	4	36	theme	carboxylic	506:515	arg1	groups					517:522	carboxylic groups	506:522	carboxylic groups	506:522	DSC analysis reveals an endothermic peak due to the scission of the ionic pairs between carboxylic groups and ammonium groups, which produces a strong change on physical properties such as methanol permeability and proton conductivity.
27987892	7	37	theme	pure	944:947	arg1	membrane					952:959	a pure CH membrane	942:959	a pure CH membrane	942:959	For a pure CH membrane the conductivity is 2.44×10-3S/cm, decreasing with pectin content until the composition 50/50 (v/v), in which the conductivity drops almost one order of magnitude.
27987892	4	38	theme	DSC	418:420	arg1	analysis					422:429	DSC analysis	418:429	DSC analysis	418:429	DSC analysis reveals an endothermic peak due to the scission of the ionic pairs between carboxylic groups and ammonium groups, which produces a strong change on physical properties such as methanol permeability and proton conductivity.
27987892	3	39	theme	CH/PEC	304:309	arg1	membranes					311:319	CH/PEC membranes	304:319	CH/PEC membranes	304:319	The results show that CH/PEC membranes display structural changes on the chemical and physical properties as a function of composition.
27987892	5	40	from	×10-6cm2/s	732:741	arg1	Pectin					709:714	Pectin	709:714	Pectin	709:714	The methanol permeability decreases with the amount of Pectin from (4.24±0.04)×10-6cm2/s for pure Chitosan membrane to (1.51±0.03)×10-6cm2/s for blend CH/PEC membranes when the amount of Pectin is 50% (v/v).
27987892	5	40	from	×10-6cm2/s	732:741	arg1	amount					699:704	the amount	695:704	the amount of Pectin from (4.24±0.04)×10-6cm2/s for pure Chitosan membrane to (1.51±0.03)×10-6cm2/s for blend CH/PEC membranes	695:820	The methanol permeability decreases with the amount of Pectin from (4.24±0.04)×10-6cm2/s for pure Chitosan membrane to (1.51±0.03)×10-6cm2/s for blend CH/PEC membranes when the amount of Pectin is 50% (v/v).
27987892	2	41	theme	obtained	207:214	arg1	samples					216:222	The obtained samples	203:222	The obtained samples	203:222	The obtained samples were cross-linked and sulfonated before characterization.
27987892	0	42	theme	Pectin	13:18	arg1	Influence					0:8	Influence	0:8	Influence of Pectin as a green polymer electrolyte on the transport properties of Chitosan-Pectin membranes.	0:107	Influence of Pectin as a green polymer electrolyte on the transport properties of Chitosan-Pectin membranes.
27987892	4	43	theme	physical	579:586	arg1	permeability					616:627	methanol permeability	607:627	methanol permeability	607:627	DSC analysis reveals an endothermic peak due to the scission of the ionic pairs between carboxylic groups and ammonium groups, which produces a strong change on physical properties such as methanol permeability and proton conductivity.
27987892	4	43	theme	physical	579:586	arg1	conductivity					640:651	proton conductivity	633:651	proton conductivity	633:651	DSC analysis reveals an endothermic peak due to the scission of the ionic pairs between carboxylic groups and ammonium groups, which produces a strong change on physical properties such as methanol permeability and proton conductivity.
27987892	4	43	theme	physical	579:586	arg1	properties					588:597	physical properties	579:597	physical properties such as methanol permeability and proton conductivity	579:651	DSC analysis reveals an endothermic peak due to the scission of the ionic pairs between carboxylic groups and ammonium groups, which produces a strong change on physical properties such as methanol permeability and proton conductivity.
29108390	8	0	theme	Truncated-O-glycan	1143:1160	arg1	score					1162:1166	high Truncated-O-glycan score	1138:1166	high Truncated-O-glycan score than among the 203 (50.6%) patients with low score	1138:1217	The OS and RFS were shortened among the 198 (49.4%) patients with high Truncated-O-glycan score than among the 203 (50.6%) patients with low score (hazard ratio for OS, 7.060; 95% confidence interval [CI]: 2.765 to 18.027; p <0.001; for RFS, 4.612; 95% CI: 2.141 to 9.931; p <0.001).
29108390	12	1	theme	localized	1798:1806	arg1	patients					1814:1821	localized ccRCC patients	1798:1821	localized ccRCC patients	1798:1821	In summary, the high Truncated-O-glycan score could predict adverse clinical outcome in localized ccRCC patients after surgery.
29108390	2	2	theme	truncated	360:368	arg1	O-glycans					370:378	each truncated O-glycans	355:378	each truncated O-glycans	355:378	The study aims to evaluate the abundance of each truncated O-glycans and its clinical significance in postoperative patients with localized clear-cell renal cell carcinoma (ccRCC).
29108390	8	3	dep	ratio	1227:1231	arg1	[CI					1272:1274	7.060; 95% confidence interval [CI	1241:1274	7.060; 95% confidence interval [CI	1241:1274	The OS and RFS were shortened among the 198 (49.4%) patients with high Truncated-O-glycan score than among the 203 (50.6%) patients with low score (hazard ratio for OS, 7.060; 95% confidence interval [CI]: 2.765 to 18.027; p <0.001; for RFS, 4.612; 95% CI: 2.141 to 9.931; p <0.001).
29108390	3	4	from	expression	543:552	arg1	specimens					586:594	tumor specimens	580:594	tumor specimens from 401 patients with localized ccRCC	580:633	We used immunohistochemical testing to analyze the expression of truncated O-glycans in tumor specimens from 401 patients with localized ccRCC.
29108390	3	4	from	expression	543:552	arg1	patients					605:612	401 patients	601:612	401 patients with localized ccRCC	601:633	We used immunohistochemical testing to analyze the expression of truncated O-glycans in tumor specimens from 401 patients with localized ccRCC.
29108390	3	5	theme	tumor	580:584	arg1	specimens					586:594	tumor specimens	580:594	tumor specimens from 401 patients with localized ccRCC	580:633	We used immunohistochemical testing to analyze the expression of truncated O-glycans in tumor specimens from 401 patients with localized ccRCC.
29108390	12	6	theme	adverse	1770:1776	arg1	outcome					1787:1793	adverse clinical outcome	1770:1793	adverse clinical outcome in localized ccRCC patients	1770:1821	In summary, the high Truncated-O-glycan score could predict adverse clinical outcome in localized ccRCC patients after surgery.
29108390	2	7	with	patients	427:434	arg1	carcinoma					473:481	localized clear-cell renal cell carcinoma	441:481	localized clear-cell renal cell carcinoma (ccRCC)	441:489	The study aims to evaluate the abundance of each truncated O-glycans and its clinical significance in postoperative patients with localized clear-cell renal cell carcinoma (ccRCC).
29108390	2	7	with	patients	427:434	arg1	ccRCC					484:488	ccRCC	484:488	ccRCC	484:488	The study aims to evaluate the abundance of each truncated O-glycans and its clinical significance in postoperative patients with localized clear-cell renal cell carcinoma (ccRCC).
29108390	8	8	dep	shortened	1092:1100	arg1	18.027					1287:1292	18.027	1287:1292	18.027	1287:1292	The OS and RFS were shortened among the 198 (49.4%) patients with high Truncated-O-glycan score than among the 203 (50.6%) patients with low score (hazard ratio for OS, 7.060; 95% confidence interval [CI]: 2.765 to 18.027; p <0.001; for RFS, 4.612; 95% CI: 2.141 to 9.931; p <0.001).
29108390	8	8	dep	shortened	1092:1100	arg1	ratio					1227:1231	hazard ratio	1220:1231	hazard ratio for OS	1220:1238	The OS and RFS were shortened among the 198 (49.4%) patients with high Truncated-O-glycan score than among the 203 (50.6%) patients with low score (hazard ratio for OS, 7.060; 95% confidence interval [CI]: 2.765 to 18.027; p <0.001; for RFS, 4.612; 95% CI: 2.141 to 9.931; p <0.001).
29108390	3	9	theme	O-glycans	567:575	arg1	expression					543:552	the expression	539:552	the expression of truncated O-glycans in tumor specimens from 401 patients with localized ccRCC	539:633	We used immunohistochemical testing to analyze the expression of truncated O-glycans in tumor specimens from 401 patients with localized ccRCC.
29108390	6	10	theme	prognostic	915:924	arg1	factors					926:932	prognostic factors	915:932	prognostic factors	915:932	Receiver operating characteristic (ROC) was applied to assess the impact of prognostic factors on overall survival (OS) and recurrence-free survival (RFS).
29108390	9	11	from	difference	1368:1377	arg1	group					1441:1445	low score group	1431:1445	low score group (p = 0.987)	1431:1457	There is no difference between low-risk patients and high-risk patients in low score group (p = 0.987).
29108390	9	11	from	difference	1368:1377	arg1	p					1448:1448	p = 0.987	1448:1456	p = 0.987	1448:1456	There is no difference between low-risk patients and high-risk patients in low score group (p = 0.987).
29108390	12	12	theme	Truncated-O-glycan	1731:1748	arg1	score					1750:1754	the high Truncated-O-glycan score	1722:1754	the high Truncated-O-glycan score	1722:1754	In summary, the high Truncated-O-glycan score could predict adverse clinical outcome in localized ccRCC patients after surgery.
29108390	6	13	from	impact	905:910	arg1	survival					979:986	recurrence-free survival	963:986	recurrence-free survival (RFS)	963:992	Receiver operating characteristic (ROC) was applied to assess the impact of prognostic factors on overall survival (OS) and recurrence-free survival (RFS).
29108390	6	13	from	impact	905:910	arg1	OS					955:956	OS	955:956	OS	955:956	Receiver operating characteristic (ROC) was applied to assess the impact of prognostic factors on overall survival (OS) and recurrence-free survival (RFS).
29108390	6	13	from	impact	905:910	arg1	RFS					989:991	RFS	989:991	RFS	989:991	Receiver operating characteristic (ROC) was applied to assess the impact of prognostic factors on overall survival (OS) and recurrence-free survival (RFS).
29108390	6	13	from	impact	905:910	arg1	survival					945:952	overall survival	937:952	overall survival (OS)	937:957	Receiver operating characteristic (ROC) was applied to assess the impact of prognostic factors on overall survival (OS) and recurrence-free survival (RFS).
29108390	8	14	dep	18.027	1287:1292	arg1	%					1323:1323	4.612; 95%	1314:1323	4.612; 95% CI: 2.141 to 9.931	1314:1342	The OS and RFS were shortened among the 198 (49.4%) patients with high Truncated-O-glycan score than among the 203 (50.6%) patients with low score (hazard ratio for OS, 7.060; 95% confidence interval [CI]: 2.765 to 18.027; p <0.001; for RFS, 4.612; 95% CI: 2.141 to 9.931; p <0.001).
29108390	8	14	dep	18.027	1287:1292	arg1	p					1345:1345	p <0.001	1345:1352	p <0.001	1345:1352	The OS and RFS were shortened among the 198 (49.4%) patients with high Truncated-O-glycan score than among the 203 (50.6%) patients with low score (hazard ratio for OS, 7.060; 95% confidence interval [CI]: 2.765 to 18.027; p <0.001; for RFS, 4.612; 95% CI: 2.141 to 9.931; p <0.001).
29108390	8	14	dep	18.027	1287:1292	arg1	p					1295:1295	p <0.001	1295:1302	p <0.001; for RFS	1295:1311	The OS and RFS were shortened among the 198 (49.4%) patients with high Truncated-O-glycan score than among the 203 (50.6%) patients with low score (hazard ratio for OS, 7.060; 95% confidence interval [CI]: 2.765 to 18.027; p <0.001; for RFS, 4.612; 95% CI: 2.141 to 9.931; p <0.001).
29108390	8	14	dep	18.027	1287:1292	arg1	to					1284:1285	to	1284:1285	to	1284:1285	The OS and RFS were shortened among the 198 (49.4%) patients with high Truncated-O-glycan score than among the 203 (50.6%) patients with low score (hazard ratio for OS, 7.060; 95% confidence interval [CI]: 2.765 to 18.027; p <0.001; for RFS, 4.612; 95% CI: 2.141 to 9.931; p <0.001).
29108390	4	15	theme	expression	690:699	arg1	level					701:705	the expression level	686:705	the expression level of Tn-, sTn- and sT-antigen	686:733	Truncated-O-glycan score was built by integrating the expression level of Tn-, sTn- and sT-antigen.
29108390	5	16	theme	Cox	762:764	arg1	analysis					777:784	Kaplan-Meier survival and Cox regression analysis	736:784	analysis	777:784	Kaplan-Meier survival and Cox regression analysis were done to compare clinical outcomes in subgroups.
29108390	1	17	theme	incomplete	221:230	arg1	O-glycans					148:156	Truncated O-glycans	138:156	Truncated O-glycans	138:156	Truncated O-glycans, including Tn-antigen, sTn-antigen, T-antigen, sT-antigen, are incomplete glycosylated structures and their expression occur frequently in tumor tissue.
29108390	1	17	theme	incomplete	221:230	arg1	Tn-antigen					169:178	Tn-antigen	169:178	Tn-antigen	169:178	Truncated O-glycans, including Tn-antigen, sTn-antigen, T-antigen, sT-antigen, are incomplete glycosylated structures and their expression occur frequently in tumor tissue.
29108390	1	17	theme	incomplete	221:230	arg1	structures					245:254	incomplete glycosylated structures	221:254	incomplete glycosylated structures	221:254	Truncated O-glycans, including Tn-antigen, sTn-antigen, T-antigen, sT-antigen, are incomplete glycosylated structures and their expression occur frequently in tumor tissue.
29108390	8	18	theme	low	1209:1211	arg1	score					1213:1217	low score	1209:1217	low score	1209:1217	The OS and RFS were shortened among the 198 (49.4%) patients with high Truncated-O-glycan score than among the 203 (50.6%) patients with low score (hazard ratio for OS, 7.060; 95% confidence interval [CI]: 2.765 to 18.027; p <0.001; for RFS, 4.612; 95% CI: 2.141 to 9.931; p <0.001).
29108390	10	19	theme	High-risk	1460:1468	arg1	patients					1470:1477	High-risk patients	1460:1477	High-risk patients with low score	1460:1492	High-risk patients with low score showed a better prognosis than low-risk patient with high score (p = 0.029).
29108390	3	20	theme	localized	619:627	arg1	ccRCC					629:633	localized ccRCC	619:633	localized ccRCC	619:633	We used immunohistochemical testing to analyze the expression of truncated O-glycans in tumor specimens from 401 patients with localized ccRCC.
29108390	4	21	theme	sTn-	715:718	arg1	level					701:705	the expression level	686:705	the expression level of Tn-, sTn- and sT-antigen	686:733	Truncated-O-glycan score was built by integrating the expression level of Tn-, sTn- and sT-antigen.
29108390	8	22	theme	confidence	1252:1261	arg1	[CI					1272:1274	7.060; 95% confidence interval [CI	1241:1274	7.060; 95% confidence interval [CI	1241:1274	The OS and RFS were shortened among the 198 (49.4%) patients with high Truncated-O-glycan score than among the 203 (50.6%) patients with low score (hazard ratio for OS, 7.060; 95% confidence interval [CI]: 2.765 to 18.027; p <0.001; for RFS, 4.612; 95% CI: 2.141 to 9.931; p <0.001).
29108390	11	23	theme	Truncated-O-glycan	1575:1592	arg1	score					1594:1598	The Truncated-O-glycan score	1571:1598	The Truncated-O-glycan score	1571:1598	The Truncated-O-glycan score showed better prognostic value for OS (AUC: 0.739, p = 0.003) and RFS (AUC: 0.719, p = 0.003) than TNM stage.
29108390	1	24	theme	Truncated	138:146	arg1	O-glycans					148:156	Truncated O-glycans	138:156	Truncated O-glycans	138:156	Truncated O-glycans, including Tn-antigen, sTn-antigen, T-antigen, sT-antigen, are incomplete glycosylated structures and their expression occur frequently in tumor tissue.
29108390	1	24	theme	Truncated	138:146	arg1	Tn-antigen					169:178	Tn-antigen	169:178	Tn-antigen	169:178	Truncated O-glycans, including Tn-antigen, sTn-antigen, T-antigen, sT-antigen, are incomplete glycosylated structures and their expression occur frequently in tumor tissue.
29108390	1	24	theme	Truncated	138:146	arg1	structures					245:254	incomplete glycosylated structures	221:254	incomplete glycosylated structures	221:254	Truncated O-glycans, including Tn-antigen, sTn-antigen, T-antigen, sT-antigen, are incomplete glycosylated structures and their expression occur frequently in tumor tissue.
29108390	3	25	from	patients	605:612	arg1	expression					543:552	the expression	539:552	the expression of truncated O-glycans in tumor specimens from 401 patients with localized ccRCC	539:633	We used immunohistochemical testing to analyze the expression of truncated O-glycans in tumor specimens from 401 patients with localized ccRCC.
29108390	3	25	from	patients	605:612	arg1	specimens					586:594	tumor specimens	580:594	tumor specimens from 401 patients with localized ccRCC	580:633	We used immunohistochemical testing to analyze the expression of truncated O-glycans in tumor specimens from 401 patients with localized ccRCC.
29108390	9	26	theme	low-risk	1387:1394	arg1	patients					1396:1403	low-risk patients	1387:1403	low-risk patients	1387:1403	There is no difference between low-risk patients and high-risk patients in low score group (p = 0.987).
29108390	11	27	theme	prognostic	1614:1623	arg1	value					1625:1629	better prognostic value	1607:1629	better prognostic value for OS (AUC: 0.739, p = 0.003) and RFS (AUC: 0.719, p = 0.003)	1607:1692	The Truncated-O-glycan score showed better prognostic value for OS (AUC: 0.739, p = 0.003) and RFS (AUC: 0.719, p = 0.003) than TNM stage.
29108390	0	28	theme	localized	81:89	arg1	carcinoma					113:121	localized clear-cell renal cell carcinoma	81:121	localized clear-cell renal cell carcinoma after surgery	81:135	High truncated-O-glycan score predicts adverse clinical outcome in patients with localized clear-cell renal cell carcinoma after surgery.
29108390	0	29	theme	renal	102:106	arg1	carcinoma					113:121	localized clear-cell renal cell carcinoma	81:121	localized clear-cell renal cell carcinoma after surgery	81:135	High truncated-O-glycan score predicts adverse clinical outcome in patients with localized clear-cell renal cell carcinoma after surgery.
29108390	0	30	from	outcome	56:62	arg1	patients					67:74	patients	67:74	patients with localized clear-cell renal cell carcinoma after surgery	67:135	High truncated-O-glycan score predicts adverse clinical outcome in patients with localized clear-cell renal cell carcinoma after surgery.
29108390	2	31	theme	localized	441:449	arg1	carcinoma					473:481	localized clear-cell renal cell carcinoma	441:481	localized clear-cell renal cell carcinoma (ccRCC)	441:489	The study aims to evaluate the abundance of each truncated O-glycans and its clinical significance in postoperative patients with localized clear-cell renal cell carcinoma (ccRCC).
29108390	2	31	theme	localized	441:449	arg1	ccRCC					484:488	ccRCC	484:488	ccRCC	484:488	The study aims to evaluate the abundance of each truncated O-glycans and its clinical significance in postoperative patients with localized clear-cell renal cell carcinoma (ccRCC).
29108390	2	32	theme	postoperative	413:425	arg1	patients					427:434	postoperative patients	413:434	postoperative patients with localized clear-cell renal cell carcinoma (ccRCC)	413:489	The study aims to evaluate the abundance of each truncated O-glycans and its clinical significance in postoperative patients with localized clear-cell renal cell carcinoma (ccRCC).
29108390	9	33	theme	low	1431:1433	arg1	group					1441:1445	low score group	1431:1445	low score group (p = 0.987)	1431:1457	There is no difference between low-risk patients and high-risk patients in low score group (p = 0.987).
29108390	9	33	theme	low	1431:1433	arg1	p					1448:1448	p = 0.987	1448:1456	p = 0.987	1448:1456	There is no difference between low-risk patients and high-risk patients in low score group (p = 0.987).
29108390	0	34	theme	High	0:3	arg1	score					24:28	High truncated-O-glycan score	0:28	High truncated-O-glycan score	0:28	High truncated-O-glycan score predicts adverse clinical outcome in patients with localized clear-cell renal cell carcinoma after surgery.
29108390	1	35	theme	tumor	297:301	arg1	tissue					303:308	tumor tissue	297:308	tumor tissue	297:308	Truncated O-glycans, including Tn-antigen, sTn-antigen, T-antigen, sT-antigen, are incomplete glycosylated structures and their expression occur frequently in tumor tissue.
29108390	2	36	theme	cell	468:471	arg1	carcinoma					473:481	localized clear-cell renal cell carcinoma	441:481	localized clear-cell renal cell carcinoma (ccRCC)	441:489	The study aims to evaluate the abundance of each truncated O-glycans and its clinical significance in postoperative patients with localized clear-cell renal cell carcinoma (ccRCC).
29108390	2	36	theme	cell	468:471	arg1	ccRCC					484:488	ccRCC	484:488	ccRCC	484:488	The study aims to evaluate the abundance of each truncated O-glycans and its clinical significance in postoperative patients with localized clear-cell renal cell carcinoma (ccRCC).
29108390	2	37	theme	clear-cell	451:460	arg1	carcinoma					473:481	localized clear-cell renal cell carcinoma	441:481	localized clear-cell renal cell carcinoma (ccRCC)	441:489	The study aims to evaluate the abundance of each truncated O-glycans and its clinical significance in postoperative patients with localized clear-cell renal cell carcinoma (ccRCC).
29108390	2	37	theme	clear-cell	451:460	arg1	ccRCC					484:488	ccRCC	484:488	ccRCC	484:488	The study aims to evaluate the abundance of each truncated O-glycans and its clinical significance in postoperative patients with localized clear-cell renal cell carcinoma (ccRCC).
29108390	3	38	with	patients	605:612	arg1	ccRCC					629:633	localized ccRCC	619:633	localized ccRCC	619:633	We used immunohistochemical testing to analyze the expression of truncated O-glycans in tumor specimens from 401 patients with localized ccRCC.
29108390	0	39	theme	adverse	39:45	arg1	outcome					56:62	adverse clinical outcome	39:62	adverse clinical outcome in patients with localized clear-cell renal cell carcinoma after surgery	39:135	High truncated-O-glycan score predicts adverse clinical outcome in patients with localized clear-cell renal cell carcinoma after surgery.
29108390	2	40	from	significance	397:408	arg1	patients					427:434	postoperative patients	413:434	postoperative patients with localized clear-cell renal cell carcinoma (ccRCC)	413:489	The study aims to evaluate the abundance of each truncated O-glycans and its clinical significance in postoperative patients with localized clear-cell renal cell carcinoma (ccRCC).
29108390	10	41	with	patient	1534:1540	arg1	score					1552:1556	high score	1547:1556	high score (p = 0.029)	1547:1568	High-risk patients with low score showed a better prognosis than low-risk patient with high score (p = 0.029).
29108390	10	42	theme	high	1547:1550	arg1	score					1552:1556	high score	1547:1556	high score (p = 0.029)	1547:1568	High-risk patients with low score showed a better prognosis than low-risk patient with high score (p = 0.029).
29108390	1	43	theme	glycosylated	232:243	arg1	O-glycans					148:156	Truncated O-glycans	138:156	Truncated O-glycans	138:156	Truncated O-glycans, including Tn-antigen, sTn-antigen, T-antigen, sT-antigen, are incomplete glycosylated structures and their expression occur frequently in tumor tissue.
29108390	1	43	theme	glycosylated	232:243	arg1	Tn-antigen					169:178	Tn-antigen	169:178	Tn-antigen	169:178	Truncated O-glycans, including Tn-antigen, sTn-antigen, T-antigen, sT-antigen, are incomplete glycosylated structures and their expression occur frequently in tumor tissue.
29108390	1	43	theme	glycosylated	232:243	arg1	structures					245:254	incomplete glycosylated structures	221:254	incomplete glycosylated structures	221:254	Truncated O-glycans, including Tn-antigen, sTn-antigen, T-antigen, sT-antigen, are incomplete glycosylated structures and their expression occur frequently in tumor tissue.
29108390	10	44	with	patients	1470:1477	arg1	score					1488:1492	low score	1484:1492	low score	1484:1492	High-risk patients with low score showed a better prognosis than low-risk patient with high score (p = 0.029).
29108390	8	45	theme	interval	1263:1270	arg1	[CI					1272:1274	7.060; 95% confidence interval [CI	1241:1274	7.060; 95% confidence interval [CI	1241:1274	The OS and RFS were shortened among the 198 (49.4%) patients with high Truncated-O-glycan score than among the 203 (50.6%) patients with low score (hazard ratio for OS, 7.060; 95% confidence interval [CI]: 2.765 to 18.027; p <0.001; for RFS, 4.612; 95% CI: 2.141 to 9.931; p <0.001).
29108390	4	46	theme	Truncated-O-glycan	636:653	arg1	score					655:659	Truncated-O-glycan score	636:659	Truncated-O-glycan score	636:659	Truncated-O-glycan score was built by integrating the expression level of Tn-, sTn- and sT-antigen.
29108390	12	47	theme	clinical	1778:1785	arg1	outcome					1787:1793	adverse clinical outcome	1770:1793	adverse clinical outcome in localized ccRCC patients	1770:1821	In summary, the high Truncated-O-glycan score could predict adverse clinical outcome in localized ccRCC patients after surgery.
29108390	5	48	theme	clinical	807:814	arg1	outcomes					816:823	clinical outcomes	807:823	clinical outcomes in subgroups	807:836	Kaplan-Meier survival and Cox regression analysis were done to compare clinical outcomes in subgroups.
29108390	2	49	theme	O-glycans	370:378	arg1	abundance					342:350	the abundance	338:350	the abundance of each truncated O-glycans	338:378	The study aims to evaluate the abundance of each truncated O-glycans and its clinical significance in postoperative patients with localized clear-cell renal cell carcinoma (ccRCC).
29108390	2	49	theme	O-glycans	370:378	arg1	significance					397:408	its clinical significance	384:408	its clinical significance in postoperative patients with localized clear-cell renal cell carcinoma (ccRCC)	384:489	The study aims to evaluate the abundance of each truncated O-glycans and its clinical significance in postoperative patients with localized clear-cell renal cell carcinoma (ccRCC).
29108390	8	50	theme	high	1138:1141	arg1	score					1162:1166	high Truncated-O-glycan score	1138:1166	high Truncated-O-glycan score than among the 203 (50.6%) patients with low score	1138:1217	The OS and RFS were shortened among the 198 (49.4%) patients with high Truncated-O-glycan score than among the 203 (50.6%) patients with low score (hazard ratio for OS, 7.060; 95% confidence interval [CI]: 2.765 to 18.027; p <0.001; for RFS, 4.612; 95% CI: 2.141 to 9.931; p <0.001).
29108390	12	51	from	outcome	1787:1793	arg1	patients					1814:1821	localized ccRCC patients	1798:1821	localized ccRCC patients	1798:1821	In summary, the high Truncated-O-glycan score could predict adverse clinical outcome in localized ccRCC patients after surgery.
29108390	2	52	from	abundance	342:350	arg1	patients					427:434	postoperative patients	413:434	postoperative patients with localized clear-cell renal cell carcinoma (ccRCC)	413:489	The study aims to evaluate the abundance of each truncated O-glycans and its clinical significance in postoperative patients with localized clear-cell renal cell carcinoma (ccRCC).
29108390	6	53	theme	overall	937:943	arg1	OS					955:956	OS	955:956	OS	955:956	Receiver operating characteristic (ROC) was applied to assess the impact of prognostic factors on overall survival (OS) and recurrence-free survival (RFS).
29108390	6	53	theme	overall	937:943	arg1	survival					945:952	overall survival	937:952	overall survival (OS)	937:957	Receiver operating characteristic (ROC) was applied to assess the impact of prognostic factors on overall survival (OS) and recurrence-free survival (RFS).
29108390	0	54	with	patients	67:74	arg1	carcinoma					113:121	localized clear-cell renal cell carcinoma	81:121	localized clear-cell renal cell carcinoma after surgery	81:135	High truncated-O-glycan score predicts adverse clinical outcome in patients with localized clear-cell renal cell carcinoma after surgery.
29108390	3	55	theme	truncated	557:565	arg1	O-glycans					567:575	truncated O-glycans	557:575	truncated O-glycans	557:575	We used immunohistochemical testing to analyze the expression of truncated O-glycans in tumor specimens from 401 patients with localized ccRCC.
29108390	6	56	theme	factors	926:932	arg1	impact					905:910	the impact	901:910	the impact of prognostic factors on overall survival (OS) and recurrence-free survival (RFS)	901:992	Receiver operating characteristic (ROC) was applied to assess the impact of prognostic factors on overall survival (OS) and recurrence-free survival (RFS).
29108390	8	57	theme	hazard	1220:1225	arg1	ratio					1227:1231	hazard ratio	1220:1231	hazard ratio for OS	1220:1238	The OS and RFS were shortened among the 198 (49.4%) patients with high Truncated-O-glycan score than among the 203 (50.6%) patients with low score (hazard ratio for OS, 7.060; 95% confidence interval [CI]: 2.765 to 18.027; p <0.001; for RFS, 4.612; 95% CI: 2.141 to 9.931; p <0.001).
29108390	5	58	theme	Kaplan-Meier	736:747	arg1	survival					749:756	Kaplan-Meier survival and Cox regression analysis	736:784	survival	749:756	Kaplan-Meier survival and Cox regression analysis were done to compare clinical outcomes in subgroups.
29108390	12	59	theme	high	1726:1729	arg1	score					1750:1754	the high Truncated-O-glycan score	1722:1754	the high Truncated-O-glycan score	1722:1754	In summary, the high Truncated-O-glycan score could predict adverse clinical outcome in localized ccRCC patients after surgery.
29108390	10	60	theme	low	1484:1486	arg1	score					1488:1492	low score	1484:1492	low score	1484:1492	High-risk patients with low score showed a better prognosis than low-risk patient with high score (p = 0.029).
29108390	4	61	theme	Tn-	710:712	arg1	level					701:705	the expression level	686:705	the expression level of Tn-, sTn- and sT-antigen	686:733	Truncated-O-glycan score was built by integrating the expression level of Tn-, sTn- and sT-antigen.
29108390	5	62	theme	regression	766:775	arg1	analysis					777:784	Kaplan-Meier survival and Cox regression analysis	736:784	analysis	777:784	Kaplan-Meier survival and Cox regression analysis were done to compare clinical outcomes in subgroups.
29108390	6	63	theme	recurrence-free	963:977	arg1	survival					979:986	recurrence-free survival	963:986	recurrence-free survival (RFS)	963:992	Receiver operating characteristic (ROC) was applied to assess the impact of prognostic factors on overall survival (OS) and recurrence-free survival (RFS).
29108390	6	63	theme	recurrence-free	963:977	arg1	RFS					989:991	RFS	989:991	RFS	989:991	Receiver operating characteristic (ROC) was applied to assess the impact of prognostic factors on overall survival (OS) and recurrence-free survival (RFS).
29108390	7	64	theme	independent	1043:1053	arg1	Tn-					1018:1020	Tn-	1018:1020	Tn-	1018:1020	The results identified Tn-, sTn-, sT-antigen as independent prognosticators.
29108390	7	64	theme	independent	1043:1053	arg1	prognosticators					1055:1069	independent prognosticators	1043:1069	independent prognosticators	1043:1069	The results identified Tn-, sTn-, sT-antigen as independent prognosticators.
29108390	5	65	from	outcomes	816:823	arg1	subgroups					828:836	subgroups	828:836	subgroups	828:836	Kaplan-Meier survival and Cox regression analysis were done to compare clinical outcomes in subgroups.
29108390	8	66	dep	%	1323:1323	arg1	to					1335:1336	to	1335:1336	to	1335:1336	The OS and RFS were shortened among the 198 (49.4%) patients with high Truncated-O-glycan score than among the 203 (50.6%) patients with low score (hazard ratio for OS, 7.060; 95% confidence interval [CI]: 2.765 to 18.027; p <0.001; for RFS, 4.612; 95% CI: 2.141 to 9.931; p <0.001).
29108390	8	67	theme	%	1250:1250	arg1	[CI					1272:1274	7.060; 95% confidence interval [CI	1241:1274	7.060; 95% confidence interval [CI	1241:1274	The OS and RFS were shortened among the 198 (49.4%) patients with high Truncated-O-glycan score than among the 203 (50.6%) patients with low score (hazard ratio for OS, 7.060; 95% confidence interval [CI]: 2.765 to 18.027; p <0.001; for RFS, 4.612; 95% CI: 2.141 to 9.931; p <0.001).
29108390	11	68	theme	=	1653:1653	arg1	p					1651:1651	p = 0.003	1651:1659	p = 0.003	1651:1659	The Truncated-O-glycan score showed better prognostic value for OS (AUC: 0.739, p = 0.003) and RFS (AUC: 0.719, p = 0.003) than TNM stage.
29108390	11	69	theme	TNM	1699:1701	arg1	stage					1703:1707	TNM stage	1699:1707	TNM stage	1699:1707	The Truncated-O-glycan score showed better prognostic value for OS (AUC: 0.739, p = 0.003) and RFS (AUC: 0.719, p = 0.003) than TNM stage.
29108390	8	70	with	patients	1195:1202	arg1	score					1213:1217	low score	1209:1217	low score	1209:1217	The OS and RFS were shortened among the 198 (49.4%) patients with high Truncated-O-glycan score than among the 203 (50.6%) patients with low score (hazard ratio for OS, 7.060; 95% confidence interval [CI]: 2.765 to 18.027; p <0.001; for RFS, 4.612; 95% CI: 2.141 to 9.931; p <0.001).
29108390	12	71	theme	ccRCC	1808:1812	arg1	patients					1814:1821	localized ccRCC patients	1798:1821	localized ccRCC patients	1798:1821	In summary, the high Truncated-O-glycan score could predict adverse clinical outcome in localized ccRCC patients after surgery.
29108390	0	72	theme	clear-cell	91:100	arg1	carcinoma					113:121	localized clear-cell renal cell carcinoma	81:121	localized clear-cell renal cell carcinoma after surgery	81:135	High truncated-O-glycan score predicts adverse clinical outcome in patients with localized clear-cell renal cell carcinoma after surgery.
29108390	4	73	theme	sT-antigen	724:733	arg1	level					701:705	the expression level	686:705	the expression level of Tn-, sTn- and sT-antigen	686:733	Truncated-O-glycan score was built by integrating the expression level of Tn-, sTn- and sT-antigen.
29108390	0	74	theme	cell	108:111	arg1	carcinoma					113:121	localized clear-cell renal cell carcinoma	81:121	localized clear-cell renal cell carcinoma after surgery	81:135	High truncated-O-glycan score predicts adverse clinical outcome in patients with localized clear-cell renal cell carcinoma after surgery.
29108390	11	75	dep	AUC	1639:1641	arg1	0.739					1644:1648	0.739	1644:1648	0.739	1644:1648	The Truncated-O-glycan score showed better prognostic value for OS (AUC: 0.739, p = 0.003) and RFS (AUC: 0.719, p = 0.003) than TNM stage.
29108390	11	75	dep	AUC	1639:1641	arg1	p					1651:1651	p = 0.003	1651:1659	p = 0.003	1651:1659	The Truncated-O-glycan score showed better prognostic value for OS (AUC: 0.739, p = 0.003) and RFS (AUC: 0.719, p = 0.003) than TNM stage.
29108390	1	76	gly	glycosylated	232:243	arg1	O-glycans					148:156	Truncated O-glycans	138:156	Truncated O-glycans	138:156	Truncated O-glycans, including Tn-antigen, sTn-antigen, T-antigen, sT-antigen, are incomplete glycosylated structures and their expression occur frequently in tumor tissue.
29108390	1	76	gly	glycosylated	232:243	arg1	Tn-antigen					169:178	Tn-antigen	169:178	Tn-antigen	169:178	Truncated O-glycans, including Tn-antigen, sTn-antigen, T-antigen, sT-antigen, are incomplete glycosylated structures and their expression occur frequently in tumor tissue.
29108390	1	76	gly	glycosylated	232:243	arg1	structures					245:254	incomplete glycosylated structures	221:254	incomplete glycosylated structures	221:254	Truncated O-glycans, including Tn-antigen, sTn-antigen, T-antigen, sT-antigen, are incomplete glycosylated structures and their expression occur frequently in tumor tissue.
29108390	9	77	theme	high-risk	1409:1417	arg1	patients					1419:1426	high-risk patients	1409:1426	high-risk patients	1409:1426	There is no difference between low-risk patients and high-risk patients in low score group (p = 0.987).
29108390	11	78	theme	better	1607:1612	arg1	value					1625:1629	better prognostic value	1607:1629	better prognostic value for OS (AUC: 0.739, p = 0.003) and RFS (AUC: 0.719, p = 0.003)	1607:1692	The Truncated-O-glycan score showed better prognostic value for OS (AUC: 0.739, p = 0.003) and RFS (AUC: 0.719, p = 0.003) than TNM stage.
29108390	11	79	theme	=	1685:1685	arg1	p					1683:1683	p = 0.003	1683:1691	p = 0.003	1683:1691	The Truncated-O-glycan score showed better prognostic value for OS (AUC: 0.739, p = 0.003) and RFS (AUC: 0.719, p = 0.003) than TNM stage.
29108390	0	80	theme	truncated-O-glycan	5:22	arg1	score					24:28	High truncated-O-glycan score	0:28	High truncated-O-glycan score	0:28	High truncated-O-glycan score predicts adverse clinical outcome in patients with localized clear-cell renal cell carcinoma after surgery.
29108390	9	81	theme	score	1435:1439	arg1	group					1441:1445	low score group	1431:1445	low score group (p = 0.987)	1431:1457	There is no difference between low-risk patients and high-risk patients in low score group (p = 0.987).
29108390	9	81	theme	score	1435:1439	arg1	p					1448:1448	p = 0.987	1448:1456	p = 0.987	1448:1456	There is no difference between low-risk patients and high-risk patients in low score group (p = 0.987).
29108390	2	82	theme	clinical	388:395	arg1	significance					397:408	its clinical significance	384:408	its clinical significance in postoperative patients with localized clear-cell renal cell carcinoma (ccRCC)	384:489	The study aims to evaluate the abundance of each truncated O-glycans and its clinical significance in postoperative patients with localized clear-cell renal cell carcinoma (ccRCC).
29108390	11	83	dep	AUC	1671:1673	arg1	p					1683:1683	p = 0.003	1683:1691	p = 0.003	1683:1691	The Truncated-O-glycan score showed better prognostic value for OS (AUC: 0.739, p = 0.003) and RFS (AUC: 0.719, p = 0.003) than TNM stage.
29108390	11	83	dep	AUC	1671:1673	arg1	0.719					1676:1680	0.719	1676:1680	0.719	1676:1680	The Truncated-O-glycan score showed better prognostic value for OS (AUC: 0.739, p = 0.003) and RFS (AUC: 0.719, p = 0.003) than TNM stage.
29108390	2	84	theme	renal	462:466	arg1	carcinoma					473:481	localized clear-cell renal cell carcinoma	441:481	localized clear-cell renal cell carcinoma (ccRCC)	441:489	The study aims to evaluate the abundance of each truncated O-glycans and its clinical significance in postoperative patients with localized clear-cell renal cell carcinoma (ccRCC).
29108390	2	84	theme	renal	462:466	arg1	ccRCC					484:488	ccRCC	484:488	ccRCC	484:488	The study aims to evaluate the abundance of each truncated O-glycans and its clinical significance in postoperative patients with localized clear-cell renal cell carcinoma (ccRCC).
29108390	10	85	dep	score	1552:1556	arg1	=					1561:1561	=	1561:1561	=	1561:1561	High-risk patients with low score showed a better prognosis than low-risk patient with high score (p = 0.029).
29108390	10	86	theme	low-risk	1525:1532	arg1	patient					1534:1540	low-risk patient	1525:1540	low-risk patient with high score (p = 0.029)	1525:1568	High-risk patients with low score showed a better prognosis than low-risk patient with high score (p = 0.029).
29108390	0	87	theme	clinical	47:54	arg1	outcome					56:62	adverse clinical outcome	39:62	adverse clinical outcome in patients with localized clear-cell renal cell carcinoma after surgery	39:135	High truncated-O-glycan score predicts adverse clinical outcome in patients with localized clear-cell renal cell carcinoma after surgery.
29108390	9	88	theme	=	1450:1450	arg1	group					1441:1445	low score group	1431:1445	low score group (p = 0.987)	1431:1457	There is no difference between low-risk patients and high-risk patients in low score group (p = 0.987).
29108390	9	88	theme	=	1450:1450	arg1	p					1448:1448	p = 0.987	1448:1456	p = 0.987	1448:1456	There is no difference between low-risk patients and high-risk patients in low score group (p = 0.987).
29108390	10	89	theme	better	1503:1508	arg1	prognosis					1510:1518	a better prognosis	1501:1518	a better prognosis	1501:1518	High-risk patients with low score showed a better prognosis than low-risk patient with high score (p = 0.029).
29108390	3	90	used	used	495:498	arg2	We					492:493	We	492:493	We	492:493	We used immunohistochemical testing to analyze the expression of truncated O-glycans in tumor specimens from 401 patients with localized ccRCC.
29108390	3	91	theme	immunohistochemical	500:518	arg1	testing					520:526	immunohistochemical testing	500:526	immunohistochemical testing	500:526	We used immunohistochemical testing to analyze the expression of truncated O-glycans in tumor specimens from 401 patients with localized ccRCC.
24751274	4	0	theme	PBSA	981:984	arg1	phase					986:990	the PBSA phase	977:990	the PBSA phase	977:990	This property was related to the controlled release of model molecule (fluorescein) previously dispersed in the PLS and revealed that its release depended on the tortuosity of the PLS phase tailored by the polymer blends composition and by the limited swelling of the PLS when entrapped in the PBSA phase.
24751274	4	1	theme	controlled	720:729	arg1	release					731:737	the controlled release	716:737	the controlled release of model molecule (fluorescein) previously dispersed in the PLS	716:801	This property was related to the controlled release of model molecule (fluorescein) previously dispersed in the PLS and revealed that its release depended on the tortuosity of the PLS phase tailored by the polymer blends composition and by the limited swelling of the PLS when entrapped in the PBSA phase.
24751274	5	2	with	contact	1130:1136	arg1	foods					1173:1177	intermediate to high moisture foods	1143:1177	intermediate to high moisture foods	1143:1177	Future applications will focus on food preservatives dispersed in PBSA-PLS blends to obtain active antimicrobial packaging put in direct contact with intermediate to high moisture foods.
24751274	0	3	theme	release	97:103	arg1	films					105:109	controlled release films	86:109	controlled release films	86:109	Polybutylene succinate adipate/starch blends: a morphological study for the design of controlled release films.
24751274	1	4	theme	distinct	328:335	arg1	morphologies					337:348	distinct morphologies	328:348	distinct morphologies	328:348	Films made of plasticized starch (PLS)/poly(butylene succinate co-butylene adipate) (PBSA) blends were prepared by thermomechanical processing varying the PBSA proportions in blends to obtain biphasic materials with distinct morphologies.
24751274	4	5	theme	polymer	893:899	arg1	composition					908:918	the polymer blends composition	889:918	the polymer blends composition	889:918	This property was related to the controlled release of model molecule (fluorescein) previously dispersed in the PLS and revealed that its release depended on the tortuosity of the PLS phase tailored by the polymer blends composition and by the limited swelling of the PLS when entrapped in the PBSA phase.
24751274	1	6	with	materials	313:321	arg1	morphologies					337:348	distinct morphologies	328:348	distinct morphologies	328:348	Films made of plasticized starch (PLS)/poly(butylene succinate co-butylene adipate) (PBSA) blends were prepared by thermomechanical processing varying the PBSA proportions in blends to obtain biphasic materials with distinct morphologies.
24751274	1	7	theme	thermomechanical	227:242	arg1	processing					244:253	thermomechanical processing	227:253	thermomechanical processing varying the PBSA proportions	227:282	Films made of plasticized starch (PLS)/poly(butylene succinate co-butylene adipate) (PBSA) blends were prepared by thermomechanical processing varying the PBSA proportions in blends to obtain biphasic materials with distinct morphologies.
24751274	0	8	theme	controlled	86:95	arg1	films					105:109	controlled release films	86:109	controlled release films	86:109	Polybutylene succinate adipate/starch blends: a morphological study for the design of controlled release films.
24751274	4	9	theme	molecule	748:755	arg1	release					731:737	the controlled release	716:737	the controlled release of model molecule (fluorescein) previously dispersed in the PLS	716:801	This property was related to the controlled release of model molecule (fluorescein) previously dispersed in the PLS and revealed that its release depended on the tortuosity of the PLS phase tailored by the polymer blends composition and by the limited swelling of the PLS when entrapped in the PBSA phase.
24751274	1	10	dep	obtain	297:302	arg1	blends					287:292	blends	287:292	blends	287:292	Films made of plasticized starch (PLS)/poly(butylene succinate co-butylene adipate) (PBSA) blends were prepared by thermomechanical processing varying the PBSA proportions in blends to obtain biphasic materials with distinct morphologies.
24751274	2	11	theme	selective	392:400	arg1	extraction					402:411	selective extraction	392:411	selective extraction of each phase	392:425	These morphologies were characterized by selective extraction of each phase, microscopic observations, and selective water/oxygen permeation properties.
24751274	0	12	theme	films	105:109	arg1	design					76:81	the design	72:81	the design of controlled release films	72:109	Polybutylene succinate adipate/starch blends: a morphological study for the design of controlled release films.
24751274	5	13	dep	high	1159:1162	arg1	to					1156:1157	to	1156:1157	to	1156:1157	Future applications will focus on food preservatives dispersed in PBSA-PLS blends to obtain active antimicrobial packaging put in direct contact with intermediate to high moisture foods.
24751274	0	14	theme	succinate	13:21	arg1	blends					38:43	Polybutylene succinate adipate/starch blends	0:43	Polybutylene succinate adipate/starch blends: a morphological study for the design of controlled release films.	0:110	Polybutylene succinate adipate/starch blends: a morphological study for the design of controlled release films.
24751274	5	15	theme	direct	1123:1128	arg1	contact					1130:1136	direct contact	1123:1136	direct contact with intermediate to high moisture foods	1123:1177	Future applications will focus on food preservatives dispersed in PBSA-PLS blends to obtain active antimicrobial packaging put in direct contact with intermediate to high moisture foods.
24751274	4	16	theme	phase	871:875	arg1	tortuosity					849:858	the tortuosity	845:858	the tortuosity of the PLS phase tailored by the polymer blends composition and by the limited swelling of the PLS	845:957	This property was related to the controlled release of model molecule (fluorescein) previously dispersed in the PLS and revealed that its release depended on the tortuosity of the PLS phase tailored by the polymer blends composition and by the limited swelling of the PLS when entrapped in the PBSA phase.
24751274	3	17	theme	partial	599:605	arg1	percolation					635:645	cluster partial percolation	619:645	cluster partial percolation	619:645	These experiments allowed identifying the blend compositions corresponding to the beginning of partial continuity (cluster partial percolation) until total continuity of each phases.
24751274	3	17	theme	partial	599:605	arg1	continuity					607:616	partial continuity	599:616	partial continuity (cluster partial percolation)	599:646	These experiments allowed identifying the blend compositions corresponding to the beginning of partial continuity (cluster partial percolation) until total continuity of each phases.
24751274	1	18	theme	butylene	156:163	arg1	adipate					187:193	butylene succinate co-butylene adipate	156:193	butylene succinate co-butylene adipate	156:193	Films made of plasticized starch (PLS)/poly(butylene succinate co-butylene adipate) (PBSA) blends were prepared by thermomechanical processing varying the PBSA proportions in blends to obtain biphasic materials with distinct morphologies.
24751274	1	18	theme	butylene	156:163	arg1	starch					138:143	plasticized starch	126:143	plasticized starch (PLS)/poly(butylene succinate co-butylene adipate) (PBSA) blends	126:208	Films made of plasticized starch (PLS)/poly(butylene succinate co-butylene adipate) (PBSA) blends were prepared by thermomechanical processing varying the PBSA proportions in blends to obtain biphasic materials with distinct morphologies.
24751274	0	19	theme	Polybutylene	0:11	arg1	blends					38:43	Polybutylene succinate adipate/starch blends	0:43	Polybutylene succinate adipate/starch blends: a morphological study for the design of controlled release films.	0:110	Polybutylene succinate adipate/starch blends: a morphological study for the design of controlled release films.
24751274	4	20	theme	PLS	867:869	arg1	phase					871:875	the PLS phase	863:875	the PLS phase tailored by the polymer blends composition and by the limited swelling of the PLS	863:957	This property was related to the controlled release of model molecule (fluorescein) previously dispersed in the PLS and revealed that its release depended on the tortuosity of the PLS phase tailored by the polymer blends composition and by the limited swelling of the PLS when entrapped in the PBSA phase.
24751274	3	21	theme	continuity	607:616	arg1	beginning					586:594	the beginning	582:594	the beginning of partial continuity (cluster partial percolation) until total continuity of each phases	582:684	These experiments allowed identifying the blend compositions corresponding to the beginning of partial continuity (cluster partial percolation) until total continuity of each phases.
24751274	1	22	theme	succinate	165:173	arg1	adipate					187:193	butylene succinate co-butylene adipate	156:193	butylene succinate co-butylene adipate	156:193	Films made of plasticized starch (PLS)/poly(butylene succinate co-butylene adipate) (PBSA) blends were prepared by thermomechanical processing varying the PBSA proportions in blends to obtain biphasic materials with distinct morphologies.
24751274	1	22	theme	succinate	165:173	arg1	starch					138:143	plasticized starch	126:143	plasticized starch (PLS)/poly(butylene succinate co-butylene adipate) (PBSA) blends	126:208	Films made of plasticized starch (PLS)/poly(butylene succinate co-butylene adipate) (PBSA) blends were prepared by thermomechanical processing varying the PBSA proportions in blends to obtain biphasic materials with distinct morphologies.
24751274	2	23	theme	microscopic	428:438	arg1	observations					440:451	microscopic observations	428:451	microscopic observations	428:451	These morphologies were characterized by selective extraction of each phase, microscopic observations, and selective water/oxygen permeation properties.
24751274	4	24	theme	limited	931:937	arg1	swelling					939:946	the limited swelling	927:946	the limited swelling of the PLS	927:957	This property was related to the controlled release of model molecule (fluorescein) previously dispersed in the PLS and revealed that its release depended on the tortuosity of the PLS phase tailored by the polymer blends composition and by the limited swelling of the PLS when entrapped in the PBSA phase.
24751274	2	25	theme	phase	421:425	arg1	observations					440:451	microscopic observations	428:451	microscopic observations	428:451	These morphologies were characterized by selective extraction of each phase, microscopic observations, and selective water/oxygen permeation properties.
24751274	2	25	theme	phase	421:425	arg1	properties					492:501	selective water/oxygen permeation properties	458:501	selective water/oxygen permeation properties	458:501	These morphologies were characterized by selective extraction of each phase, microscopic observations, and selective water/oxygen permeation properties.
24751274	2	25	theme	phase	421:425	arg1	extraction					402:411	selective extraction	392:411	selective extraction of each phase	392:425	These morphologies were characterized by selective extraction of each phase, microscopic observations, and selective water/oxygen permeation properties.
24751274	0	26	theme	adipate/starch	23:36	arg1	blends					38:43	Polybutylene succinate adipate/starch blends	0:43	Polybutylene succinate adipate/starch blends: a morphological study for the design of controlled release films.	0:110	Polybutylene succinate adipate/starch blends: a morphological study for the design of controlled release films.
24751274	1	27	theme	PBSA	267:270	arg1	proportions					272:282	the PBSA proportions	263:282	the PBSA proportions	263:282	Films made of plasticized starch (PLS)/poly(butylene succinate co-butylene adipate) (PBSA) blends were prepared by thermomechanical processing varying the PBSA proportions in blends to obtain biphasic materials with distinct morphologies.
24751274	3	28	theme	phases	679:684	arg1	continuity					660:669	total continuity	654:669	total continuity of each phases	654:684	These experiments allowed identifying the blend compositions corresponding to the beginning of partial continuity (cluster partial percolation) until total continuity of each phases.
24751274	2	29	theme	permeation	481:490	arg1	properties					492:501	selective water/oxygen permeation properties	458:501	selective water/oxygen permeation properties	458:501	These morphologies were characterized by selective extraction of each phase, microscopic observations, and selective water/oxygen permeation properties.
24751274	3	30	theme	partial	627:633	arg1	percolation					635:645	cluster partial percolation	619:645	cluster partial percolation	619:645	These experiments allowed identifying the blend compositions corresponding to the beginning of partial continuity (cluster partial percolation) until total continuity of each phases.
24751274	3	30	theme	partial	627:633	arg1	continuity					607:616	partial continuity	599:616	partial continuity (cluster partial percolation)	599:646	These experiments allowed identifying the blend compositions corresponding to the beginning of partial continuity (cluster partial percolation) until total continuity of each phases.
24751274	2	31	theme	water/oxygen	468:479	arg1	properties					492:501	selective water/oxygen permeation properties	458:501	selective water/oxygen permeation properties	458:501	These morphologies were characterized by selective extraction of each phase, microscopic observations, and selective water/oxygen permeation properties.
24751274	5	32	theme	high	1159:1162	arg1	foods					1173:1177	intermediate to high moisture foods	1143:1177	intermediate to high moisture foods	1143:1177	Future applications will focus on food preservatives dispersed in PBSA-PLS blends to obtain active antimicrobial packaging put in direct contact with intermediate to high moisture foods.
24751274	5	33	theme	active	1085:1090	arg1	packaging					1106:1114	active antimicrobial packaging	1085:1114	active antimicrobial packaging put in direct contact with intermediate to high moisture foods	1085:1177	Future applications will focus on food preservatives dispersed in PBSA-PLS blends to obtain active antimicrobial packaging put in direct contact with intermediate to high moisture foods.
24751274	5	34	theme	antimicrobial	1092:1104	arg1	packaging					1106:1114	active antimicrobial packaging	1085:1114	active antimicrobial packaging put in direct contact with intermediate to high moisture foods	1085:1177	Future applications will focus on food preservatives dispersed in PBSA-PLS blends to obtain active antimicrobial packaging put in direct contact with intermediate to high moisture foods.
24751274	2	35	theme	selective	458:466	arg1	properties					492:501	selective water/oxygen permeation properties	458:501	selective water/oxygen permeation properties	458:501	These morphologies were characterized by selective extraction of each phase, microscopic observations, and selective water/oxygen permeation properties.
24751274	4	36	theme	PLS	955:957	arg1	swelling					939:946	the limited swelling	927:946	the limited swelling of the PLS	927:957	This property was related to the controlled release of model molecule (fluorescein) previously dispersed in the PLS and revealed that its release depended on the tortuosity of the PLS phase tailored by the polymer blends composition and by the limited swelling of the PLS when entrapped in the PBSA phase.
24751274	3	37	theme	each	674:677	arg1	phases					679:684	each phases	674:684	each phases	674:684	These experiments allowed identifying the blend compositions corresponding to the beginning of partial continuity (cluster partial percolation) until total continuity of each phases.
24751274	5	38	theme	food	1027:1030	arg1	preservatives					1032:1044	food preservatives	1027:1044	food preservatives dispersed in PBSA-PLS blends	1027:1073	Future applications will focus on food preservatives dispersed in PBSA-PLS blends to obtain active antimicrobial packaging put in direct contact with intermediate to high moisture foods.
24751274	4	39	theme	model	742:746	arg1	fluorescein					758:768	fluorescein	758:768	fluorescein	758:768	This property was related to the controlled release of model molecule (fluorescein) previously dispersed in the PLS and revealed that its release depended on the tortuosity of the PLS phase tailored by the polymer blends composition and by the limited swelling of the PLS when entrapped in the PBSA phase.
24751274	4	39	theme	model	742:746	arg1	molecule					748:755	model molecule	742:755	model molecule (fluorescein) previously dispersed in the PLS	742:801	This property was related to the controlled release of model molecule (fluorescein) previously dispersed in the PLS and revealed that its release depended on the tortuosity of the PLS phase tailored by the polymer blends composition and by the limited swelling of the PLS when entrapped in the PBSA phase.
24751274	0	40	theme	morphological	48:60	arg1	study					62:66	a morphological study	46:66	Polybutylene succinate adipate/starch blends: a morphological study for the design of controlled release films.	0:110	Polybutylene succinate adipate/starch blends: a morphological study for the design of controlled release films.
24751274	5	41	dep	packaging	1106:1114	arg1	put					1116:1118	put	1116:1118	put in direct contact with intermediate to high moisture foods	1116:1177	Future applications will focus on food preservatives dispersed in PBSA-PLS blends to obtain active antimicrobial packaging put in direct contact with intermediate to high moisture foods.
24751274	4	42	theme	blends	901:906	arg1	composition					908:918	the polymer blends composition	889:918	the polymer blends composition	889:918	This property was related to the controlled release of model molecule (fluorescein) previously dispersed in the PLS and revealed that its release depended on the tortuosity of the PLS phase tailored by the polymer blends composition and by the limited swelling of the PLS when entrapped in the PBSA phase.
24751274	3	43	theme	cluster	619:625	arg1	percolation					635:645	cluster partial percolation	619:645	cluster partial percolation	619:645	These experiments allowed identifying the blend compositions corresponding to the beginning of partial continuity (cluster partial percolation) until total continuity of each phases.
24751274	3	43	theme	cluster	619:625	arg1	continuity					607:616	partial continuity	599:616	partial continuity (cluster partial percolation)	599:646	These experiments allowed identifying the blend compositions corresponding to the beginning of partial continuity (cluster partial percolation) until total continuity of each phases.
24751274	3	44	theme	total	654:658	arg1	continuity					660:669	total continuity	654:669	total continuity of each phases	654:684	These experiments allowed identifying the blend compositions corresponding to the beginning of partial continuity (cluster partial percolation) until total continuity of each phases.
24751274	0	45	dep	blends	38:43	arg1	study					62:66	a morphological study	46:66	Polybutylene succinate adipate/starch blends: a morphological study for the design of controlled release films.	0:110	Polybutylene succinate adipate/starch blends: a morphological study for the design of controlled release films.
24751274	5	46	theme	PBSA-PLS	1059:1066	arg1	blends					1068:1073	PBSA-PLS blends	1059:1073	PBSA-PLS blends	1059:1073	Future applications will focus on food preservatives dispersed in PBSA-PLS blends to obtain active antimicrobial packaging put in direct contact with intermediate to high moisture foods.
24751274	5	47	theme	Future	993:998	arg1	applications					1000:1011	Future applications	993:1011	Future applications	993:1011	Future applications will focus on food preservatives dispersed in PBSA-PLS blends to obtain active antimicrobial packaging put in direct contact with intermediate to high moisture foods.
24751274	1	48	theme	plasticized	126:136	arg1	adipate					187:193	butylene succinate co-butylene adipate	156:193	butylene succinate co-butylene adipate	156:193	Films made of plasticized starch (PLS)/poly(butylene succinate co-butylene adipate) (PBSA) blends were prepared by thermomechanical processing varying the PBSA proportions in blends to obtain biphasic materials with distinct morphologies.
24751274	1	48	theme	plasticized	126:136	arg1	PBSA					197:200	PBSA	197:200	PBSA	197:200	Films made of plasticized starch (PLS)/poly(butylene succinate co-butylene adipate) (PBSA) blends were prepared by thermomechanical processing varying the PBSA proportions in blends to obtain biphasic materials with distinct morphologies.
24751274	1	48	theme	plasticized	126:136	arg1	PLS					146:148	PLS	146:148	PLS	146:148	Films made of plasticized starch (PLS)/poly(butylene succinate co-butylene adipate) (PBSA) blends were prepared by thermomechanical processing varying the PBSA proportions in blends to obtain biphasic materials with distinct morphologies.
24751274	1	48	theme	plasticized	126:136	arg1	starch					138:143	plasticized starch	126:143	plasticized starch (PLS)/poly(butylene succinate co-butylene adipate) (PBSA) blends	126:208	Films made of plasticized starch (PLS)/poly(butylene succinate co-butylene adipate) (PBSA) blends were prepared by thermomechanical processing varying the PBSA proportions in blends to obtain biphasic materials with distinct morphologies.
24751274	1	49	theme	co-butylene	175:185	arg1	adipate					187:193	butylene succinate co-butylene adipate	156:193	butylene succinate co-butylene adipate	156:193	Films made of plasticized starch (PLS)/poly(butylene succinate co-butylene adipate) (PBSA) blends were prepared by thermomechanical processing varying the PBSA proportions in blends to obtain biphasic materials with distinct morphologies.
24751274	1	49	theme	co-butylene	175:185	arg1	starch					138:143	plasticized starch	126:143	plasticized starch (PLS)/poly(butylene succinate co-butylene adipate) (PBSA) blends	126:208	Films made of plasticized starch (PLS)/poly(butylene succinate co-butylene adipate) (PBSA) blends were prepared by thermomechanical processing varying the PBSA proportions in blends to obtain biphasic materials with distinct morphologies.
24751274	1	50	theme	starch	138:143	arg1	blends					203:208	plasticized starch (PLS)/poly(butylene succinate co-butylene adipate) (PBSA) blends	126:208	plasticized starch (PLS)/poly(butylene succinate co-butylene adipate) (PBSA) blends	126:208	Films made of plasticized starch (PLS)/poly(butylene succinate co-butylene adipate) (PBSA) blends were prepared by thermomechanical processing varying the PBSA proportions in blends to obtain biphasic materials with distinct morphologies.
24751274	1	51	theme	biphasic	304:311	arg1	materials					313:321	biphasic materials	304:321	biphasic materials with distinct morphologies	304:348	Films made of plasticized starch (PLS)/poly(butylene succinate co-butylene adipate) (PBSA) blends were prepared by thermomechanical processing varying the PBSA proportions in blends to obtain biphasic materials with distinct morphologies.
24751274	3	52	theme	blend	546:550	arg1	compositions					552:563	the blend compositions	542:563	the blend compositions corresponding to the beginning of partial continuity (cluster partial percolation) until total continuity of each phases	542:684	These experiments allowed identifying the blend compositions corresponding to the beginning of partial continuity (cluster partial percolation) until total continuity of each phases.
24751274	5	53	theme	moisture	1164:1171	arg1	foods					1173:1177	intermediate to high moisture foods	1143:1177	intermediate to high moisture foods	1143:1177	Future applications will focus on food preservatives dispersed in PBSA-PLS blends to obtain active antimicrobial packaging put in direct contact with intermediate to high moisture foods.
26359989	0	0	theme	Env	81:83	arg1	States					65:70	Closed and Open States	49:70	Closed and Open States of HIV-1 Env	49:83	Broadly Neutralizing Antibody 8ANC195 Recognizes Closed and Open States of HIV-1 Env.
26359989	4	1	theme	Env/CD4/CD4-induced	918:936	arg1	complex					955:961	an Env/CD4/CD4-induced antibody/8ANC195 complex	915:961	an Env/CD4/CD4-induced antibody/8ANC195 complex	915:961	To determine whether 8ANC195 recognizes the CD4-bound open Env conformation that leads to co-receptor binding and fusion, one of several known conformations of virion-associated Env, we solved EM structures of an Env/CD4/CD4-induced antibody/8ANC195 complex.
26359989	1	2	contain	contains	117:124	arg2	epitopes					134:141	limited epitopes	126:141	limited epitopes for broadly neutralizing antibodies (bNAbs)	126:185	The HIV-1 envelope (Env) spike contains limited epitopes for broadly neutralizing antibodies (bNAbs); thus, most neutralizing antibodies are strain specific.
26359989	1	2	contain	contains	117:124	arg1	spike					111:115	The HIV-1 envelope (Env) spike	86:115	The HIV-1 envelope (Env) spike	86:115	The HIV-1 envelope (Env) spike contains limited epitopes for broadly neutralizing antibodies (bNAbs); thus, most neutralizing antibodies are strain specific.
26359989	2	3	theme	Env	428:430	arg1	subunits					432:439	the gp120 and gp41 Env subunits	409:439	subunits	432:439	The 8ANC195 epitope, defined by crystal and electron microscopy (EM) structures of bNAb 8ANC195 complexed with monomeric gp120 and trimeric Env, respectively, spans the gp120 and gp41 Env subunits.
26359989	4	4	theme	several	834:840	arg1	conformations					848:860	several known conformations	834:860	several known conformations of virion-associated Env	834:885	To determine whether 8ANC195 recognizes the CD4-bound open Env conformation that leads to co-receptor binding and fusion, one of several known conformations of virion-associated Env, we solved EM structures of an Env/CD4/CD4-induced antibody/8ANC195 complex.
26359989	5	5	theme	Env	1055:1057	arg1	plasticity					1041:1050	structural plasticity	1030:1050	structural plasticity of Env	1030:1057	8ANC195 binding partially closed the CD4-bound trimer, confirming structural plasticity of Env by revealing a previously unseen conformation.
26359989	3	6	theme	glycan	631:636	arg1	gap					645:647	a glycan shield gap	629:647	a glycan shield gap	629:647	To investigate 8ANC195's gp41 epitope at higher resolution, we solved a 3.58 Å crystal structure of 8ANC195 complexed with fully glycosylated Env trimer, revealing 8ANC195 insertion into a glycan shield gap to contact gp120 and gp41 glycans and protein residues.
26359989	3	7	theme	higher	483:488	arg1	resolution					490:499	higher resolution	483:499	higher resolution	483:499	To investigate 8ANC195's gp41 epitope at higher resolution, we solved a 3.58 Å crystal structure of 8ANC195 complexed with fully glycosylated Env trimer, revealing 8ANC195 insertion into a glycan shield gap to contact gp120 and gp41 glycans and protein residues.
26359989	3	8	from	resolution	490:499	arg1	epitope					472:478	8ANC195's gp41 epitope	457:478	8ANC195's gp41 epitope at higher resolution	457:499	To investigate 8ANC195's gp41 epitope at higher resolution, we solved a 3.58 Å crystal structure of 8ANC195 complexed with fully glycosylated Env trimer, revealing 8ANC195 insertion into a glycan shield gap to contact gp120 and gp41 glycans and protein residues.
26359989	3	9	theme	contact	652:658	arg1	gp120					660:664	contact gp120	652:664	contact gp120	652:664	To investigate 8ANC195's gp41 epitope at higher resolution, we solved a 3.58 Å crystal structure of 8ANC195 complexed with fully glycosylated Env trimer, revealing 8ANC195 insertion into a glycan shield gap to contact gp120 and gp41 glycans and protein residues.
26359989	3	10	gly	glycosylated	571:582	arg1	trimer					588:593	fully glycosylated Env trimer	565:593	fully glycosylated Env trimer	565:593	To investigate 8ANC195's gp41 epitope at higher resolution, we solved a 3.58 Å crystal structure of 8ANC195 complexed with fully glycosylated Env trimer, revealing 8ANC195 insertion into a glycan shield gap to contact gp120 and gp41 glycans and protein residues.
26359989	4	11	theme	EM	898:899	arg1	structures					901:910	EM structures	898:910	EM structures of an Env/CD4/CD4-induced antibody/8ANC195 complex	898:961	To determine whether 8ANC195 recognizes the CD4-bound open Env conformation that leads to co-receptor binding and fusion, one of several known conformations of virion-associated Env, we solved EM structures of an Env/CD4/CD4-induced antibody/8ANC195 complex.
26359989	2	12	theme	8ANC195	248:254	arg1	epitope					256:262	The 8ANC195 epitope	244:262	The 8ANC195 epitope	244:262	The 8ANC195 epitope, defined by crystal and electron microscopy (EM) structures of bNAb 8ANC195 complexed with monomeric gp120 and trimeric Env, respectively, spans the gp120 and gp41 Env subunits.
26359989	2	13	theme	monomeric	355:363	arg1	gp120					365:369	monomeric gp120	355:369	monomeric gp120	355:369	The 8ANC195 epitope, defined by crystal and electron microscopy (EM) structures of bNAb 8ANC195 complexed with monomeric gp120 and trimeric Env, respectively, spans the gp120 and gp41 Env subunits.
26359989	6	14	dep	potential	1184:1192	arg1	therapeutic					1194:1204	therapeutic	1194:1204	therapeutic	1194:1204	8ANC195's ability to bind different Env conformations suggests advantages for potential therapeutic applications.
26359989	0	15	theme	Closed	49:54	arg1	States					65:70	Closed and Open States	49:70	Closed and Open States of HIV-1 Env	49:83	Broadly Neutralizing Antibody 8ANC195 Recognizes Closed and Open States of HIV-1 Env.
26359989	6	16	theme	different	1132:1140	arg1	conformations					1146:1158	different Env conformations	1132:1158	different Env conformations	1132:1158	8ANC195's ability to bind different Env conformations suggests advantages for potential therapeutic applications.
26359989	1	17	theme	neutralizing	155:166	arg1	antibodies					168:177	broadly neutralizing antibodies	147:177	broadly neutralizing antibodies (bNAbs)	147:185	The HIV-1 envelope (Env) spike contains limited epitopes for broadly neutralizing antibodies (bNAbs); thus, most neutralizing antibodies are strain specific.
26359989	1	17	theme	neutralizing	155:166	arg1	bNAbs					180:184	bNAbs	180:184	bNAbs	180:184	The HIV-1 envelope (Env) spike contains limited epitopes for broadly neutralizing antibodies (bNAbs); thus, most neutralizing antibodies are strain specific.
26359989	0	18	theme	Neutralizing	8:19	arg1	Antibody					21:28	Broadly Neutralizing Antibody 8ANC195	0:36	Broadly Neutralizing Antibody 8ANC195	0:36	Broadly Neutralizing Antibody 8ANC195 Recognizes Closed and Open States of HIV-1 Env.
26359989	0	19	theme	HIV-1	75:79	arg1	Env					81:83	HIV-1 Env	75:83	HIV-1 Env	75:83	Broadly Neutralizing Antibody 8ANC195 Recognizes Closed and Open States of HIV-1 Env.
26359989	4	20	theme	co-receptor	795:805	arg1	one					827:829	one	827:829	one	827:829	To determine whether 8ANC195 recognizes the CD4-bound open Env conformation that leads to co-receptor binding and fusion, one of several known conformations of virion-associated Env, we solved EM structures of an Env/CD4/CD4-induced antibody/8ANC195 complex.
26359989	4	20	theme	co-receptor	795:805	arg1	conformations					848:860	several known conformations	834:860	several known conformations of virion-associated Env	834:885	To determine whether 8ANC195 recognizes the CD4-bound open Env conformation that leads to co-receptor binding and fusion, one of several known conformations of virion-associated Env, we solved EM structures of an Env/CD4/CD4-induced antibody/8ANC195 complex.
26359989	4	20	theme	co-receptor	795:805	arg1	binding					807:813	co-receptor binding	795:813	co-receptor binding	795:813	To determine whether 8ANC195 recognizes the CD4-bound open Env conformation that leads to co-receptor binding and fusion, one of several known conformations of virion-associated Env, we solved EM structures of an Env/CD4/CD4-induced antibody/8ANC195 complex.
26359989	0	21	theme	Broadly	0:6	arg1	Antibody					21:28	Broadly Neutralizing Antibody 8ANC195	0:36	Broadly Neutralizing Antibody 8ANC195	0:36	Broadly Neutralizing Antibody 8ANC195 Recognizes Closed and Open States of HIV-1 Env.
26359989	2	22	theme	crystal	276:282	arg1	structures					313:322	crystal and electron microscopy (EM) structures	276:322	crystal and electron microscopy (EM) structures of bNAb 8ANC195 complexed with monomeric gp120 and trimeric Env, respectively	276:400	The 8ANC195 epitope, defined by crystal and electron microscopy (EM) structures of bNAb 8ANC195 complexed with monomeric gp120 and trimeric Env, respectively, spans the gp120 and gp41 Env subunits.
26359989	3	23	theme	protein	687:693	arg1	residues					695:702	protein residues	687:702	protein residues	687:702	To investigate 8ANC195's gp41 epitope at higher resolution, we solved a 3.58 Å crystal structure of 8ANC195 complexed with fully glycosylated Env trimer, revealing 8ANC195 insertion into a glycan shield gap to contact gp120 and gp41 glycans and protein residues.
26359989	4	24	theme	Env	764:766	arg1	conformation					768:779	the CD4-bound open Env conformation	745:779	the CD4-bound open Env conformation that leads to co-receptor binding and fusion, one of several known conformations of virion-associated Env	745:885	To determine whether 8ANC195 recognizes the CD4-bound open Env conformation that leads to co-receptor binding and fusion, one of several known conformations of virion-associated Env, we solved EM structures of an Env/CD4/CD4-induced antibody/8ANC195 complex.
26359989	1	25	theme	HIV-1	90:94	arg1	spike					111:115	The HIV-1 envelope (Env) spike	86:115	The HIV-1 envelope (Env) spike	86:115	The HIV-1 envelope (Env) spike contains limited epitopes for broadly neutralizing antibodies (bNAbs); thus, most neutralizing antibodies are strain specific.
26359989	3	26	theme	gp41	467:470	arg1	epitope					472:478	8ANC195's gp41 epitope	457:478	8ANC195's gp41 epitope at higher resolution	457:499	To investigate 8ANC195's gp41 epitope at higher resolution, we solved a 3.58 Å crystal structure of 8ANC195 complexed with fully glycosylated Env trimer, revealing 8ANC195 insertion into a glycan shield gap to contact gp120 and gp41 glycans and protein residues.
26359989	3	27	theme	gp41	670:673	arg1	glycans					675:681	gp41 glycans	670:681	gp41 glycans	670:681	To investigate 8ANC195's gp41 epitope at higher resolution, we solved a 3.58 Å crystal structure of 8ANC195 complexed with fully glycosylated Env trimer, revealing 8ANC195 insertion into a glycan shield gap to contact gp120 and gp41 glycans and protein residues.
26359989	4	28	theme	conformations	848:860	arg1	one					827:829	one	827:829	one	827:829	To determine whether 8ANC195 recognizes the CD4-bound open Env conformation that leads to co-receptor binding and fusion, one of several known conformations of virion-associated Env, we solved EM structures of an Env/CD4/CD4-induced antibody/8ANC195 complex.
26359989	4	28	theme	conformations	848:860	arg1	binding					807:813	co-receptor binding	795:813	co-receptor binding	795:813	To determine whether 8ANC195 recognizes the CD4-bound open Env conformation that leads to co-receptor binding and fusion, one of several known conformations of virion-associated Env, we solved EM structures of an Env/CD4/CD4-induced antibody/8ANC195 complex.
26359989	4	28	theme	conformations	848:860	arg1	conformations					848:860	several known conformations	834:860	several known conformations of virion-associated Env	834:885	To determine whether 8ANC195 recognizes the CD4-bound open Env conformation that leads to co-receptor binding and fusion, one of several known conformations of virion-associated Env, we solved EM structures of an Env/CD4/CD4-induced antibody/8ANC195 complex.
26359989	5	29	theme	structural	1030:1039	arg1	plasticity					1041:1050	structural plasticity	1030:1050	structural plasticity of Env	1030:1057	8ANC195 binding partially closed the CD4-bound trimer, confirming structural plasticity of Env by revealing a previously unseen conformation.
26359989	1	30	theme	envelope	96:103	arg1	spike					111:115	The HIV-1 envelope (Env) spike	86:115	The HIV-1 envelope (Env) spike	86:115	The HIV-1 envelope (Env) spike contains limited epitopes for broadly neutralizing antibodies (bNAbs); thus, most neutralizing antibodies are strain specific.
26359989	2	31	theme	trimeric	375:382	arg1	Env					384:386	trimeric Env	375:386	trimeric Env	375:386	The 8ANC195 epitope, defined by crystal and electron microscopy (EM) structures of bNAb 8ANC195 complexed with monomeric gp120 and trimeric Env, respectively, spans the gp120 and gp41 Env subunits.
26359989	2	32	theme	gp41	423:426	arg1	subunits					432:439	the gp120 and gp41 Env subunits	409:439	subunits	432:439	The 8ANC195 epitope, defined by crystal and electron microscopy (EM) structures of bNAb 8ANC195 complexed with monomeric gp120 and trimeric Env, respectively, spans the gp120 and gp41 Env subunits.
26359989	4	33	theme	Env	883:885	arg1	conformations					848:860	several known conformations	834:860	several known conformations of virion-associated Env	834:885	To determine whether 8ANC195 recognizes the CD4-bound open Env conformation that leads to co-receptor binding and fusion, one of several known conformations of virion-associated Env, we solved EM structures of an Env/CD4/CD4-induced antibody/8ANC195 complex.
26359989	4	34	theme	open	759:762	arg1	conformation					768:779	the CD4-bound open Env conformation	745:779	the CD4-bound open Env conformation that leads to co-receptor binding and fusion, one of several known conformations of virion-associated Env	745:885	To determine whether 8ANC195 recognizes the CD4-bound open Env conformation that leads to co-receptor binding and fusion, one of several known conformations of virion-associated Env, we solved EM structures of an Env/CD4/CD4-induced antibody/8ANC195 complex.
26359989	4	35	theme	antibody/8ANC195	938:953	arg1	complex					955:961	an Env/CD4/CD4-induced antibody/8ANC195 complex	915:961	an Env/CD4/CD4-induced antibody/8ANC195 complex	915:961	To determine whether 8ANC195 recognizes the CD4-bound open Env conformation that leads to co-receptor binding and fusion, one of several known conformations of virion-associated Env, we solved EM structures of an Env/CD4/CD4-induced antibody/8ANC195 complex.
26359989	4	36	theme	virion-associated	865:881	arg1	Env					883:885	virion-associated Env	865:885	virion-associated Env	865:885	To determine whether 8ANC195 recognizes the CD4-bound open Env conformation that leads to co-receptor binding and fusion, one of several known conformations of virion-associated Env, we solved EM structures of an Env/CD4/CD4-induced antibody/8ANC195 complex.
26359989	3	37	theme	glycosylated	571:582	arg1	trimer					588:593	fully glycosylated Env trimer	565:593	fully glycosylated Env trimer	565:593	To investigate 8ANC195's gp41 epitope at higher resolution, we solved a 3.58 Å crystal structure of 8ANC195 complexed with fully glycosylated Env trimer, revealing 8ANC195 insertion into a glycan shield gap to contact gp120 and gp41 glycans and protein residues.
26359989	4	38	theme	CD4-bound	749:757	arg1	conformation					768:779	the CD4-bound open Env conformation	745:779	the CD4-bound open Env conformation that leads to co-receptor binding and fusion, one of several known conformations of virion-associated Env	745:885	To determine whether 8ANC195 recognizes the CD4-bound open Env conformation that leads to co-receptor binding and fusion, one of several known conformations of virion-associated Env, we solved EM structures of an Env/CD4/CD4-induced antibody/8ANC195 complex.
26359989	0	39	theme	Open	60:63	arg1	States					65:70	Closed and Open States	49:70	Closed and Open States of HIV-1 Env	49:83	Broadly Neutralizing Antibody 8ANC195 Recognizes Closed and Open States of HIV-1 Env.
26359989	3	40	theme	Env	584:586	arg1	trimer					588:593	fully glycosylated Env trimer	565:593	fully glycosylated Env trimer	565:593	To investigate 8ANC195's gp41 epitope at higher resolution, we solved a 3.58 Å crystal structure of 8ANC195 complexed with fully glycosylated Env trimer, revealing 8ANC195 insertion into a glycan shield gap to contact gp120 and gp41 glycans and protein residues.
26359989	3	41	theme	3.58 Å	514:519	arg1	structure					529:537	a 3.58 Å crystal structure	512:537	a 3.58 Å crystal structure of 8ANC195 complexed with fully glycosylated Env trimer	512:593	To investigate 8ANC195's gp41 epitope at higher resolution, we solved a 3.58 Å crystal structure of 8ANC195 complexed with fully glycosylated Env trimer, revealing 8ANC195 insertion into a glycan shield gap to contact gp120 and gp41 glycans and protein residues.
26359989	5	42	theme	unseen	1085:1090	arg1	conformation					1092:1103	a previously unseen conformation	1072:1103	a previously unseen conformation	1072:1103	8ANC195 binding partially closed the CD4-bound trimer, confirming structural plasticity of Env by revealing a previously unseen conformation.
26359989	5	43	theme	CD4-bound	1001:1009	arg1	trimer					1011:1016	the CD4-bound trimer	997:1016	the CD4-bound trimer	997:1016	8ANC195 binding partially closed the CD4-bound trimer, confirming structural plasticity of Env by revealing a previously unseen conformation.
26359989	3	44	theme	crystal	521:527	arg1	structure					529:537	a 3.58 Å crystal structure	512:537	a 3.58 Å crystal structure of 8ANC195 complexed with fully glycosylated Env trimer	512:593	To investigate 8ANC195's gp41 epitope at higher resolution, we solved a 3.58 Å crystal structure of 8ANC195 complexed with fully glycosylated Env trimer, revealing 8ANC195 insertion into a glycan shield gap to contact gp120 and gp41 glycans and protein residues.
26359989	1	45	theme	Env	106:108	arg1	spike					111:115	The HIV-1 envelope (Env) spike	86:115	The HIV-1 envelope (Env) spike	86:115	The HIV-1 envelope (Env) spike contains limited epitopes for broadly neutralizing antibodies (bNAbs); thus, most neutralizing antibodies are strain specific.
26359989	4	46	theme	known	842:846	arg1	conformations					848:860	several known conformations	834:860	several known conformations of virion-associated Env	834:885	To determine whether 8ANC195 recognizes the CD4-bound open Env conformation that leads to co-receptor binding and fusion, one of several known conformations of virion-associated Env, we solved EM structures of an Env/CD4/CD4-induced antibody/8ANC195 complex.
26359989	2	47	theme	microscopy	297:306	arg1	structures					313:322	crystal and electron microscopy (EM) structures	276:322	crystal and electron microscopy (EM) structures of bNAb 8ANC195 complexed with monomeric gp120 and trimeric Env, respectively	276:400	The 8ANC195 epitope, defined by crystal and electron microscopy (EM) structures of bNAb 8ANC195 complexed with monomeric gp120 and trimeric Env, respectively, spans the gp120 and gp41 Env subunits.
26359989	4	48	theme	complex	955:961	arg1	structures					901:910	EM structures	898:910	EM structures of an Env/CD4/CD4-induced antibody/8ANC195 complex	898:961	To determine whether 8ANC195 recognizes the CD4-bound open Env conformation that leads to co-receptor binding and fusion, one of several known conformations of virion-associated Env, we solved EM structures of an Env/CD4/CD4-induced antibody/8ANC195 complex.
26359989	6	49	theme	potential	1184:1192	arg1	applications					1206:1217	potential therapeutic applications	1184:1217	potential therapeutic applications	1184:1217	8ANC195's ability to bind different Env conformations suggests advantages for potential therapeutic applications.
26359989	2	50	theme	electron	288:295	arg1	EM					309:310	EM	309:310	EM	309:310	The 8ANC195 epitope, defined by crystal and electron microscopy (EM) structures of bNAb 8ANC195 complexed with monomeric gp120 and trimeric Env, respectively, spans the gp120 and gp41 Env subunits.
26359989	2	50	theme	electron	288:295	arg1	microscopy					297:306	electron microscopy	288:306	electron microscopy (EM)	288:311	The 8ANC195 epitope, defined by crystal and electron microscopy (EM) structures of bNAb 8ANC195 complexed with monomeric gp120 and trimeric Env, respectively, spans the gp120 and gp41 Env subunits.
26359989	1	51	theme	most	194:197	arg1	antibodies					212:221	most neutralizing antibodies	194:221	most neutralizing antibodies	194:221	The HIV-1 envelope (Env) spike contains limited epitopes for broadly neutralizing antibodies (bNAbs); thus, most neutralizing antibodies are strain specific.
26359989	3	52	theme	8ANC195	542:548	arg1	structure					529:537	a 3.58 Å crystal structure	512:537	a 3.58 Å crystal structure of 8ANC195 complexed with fully glycosylated Env trimer	512:593	To investigate 8ANC195's gp41 epitope at higher resolution, we solved a 3.58 Å crystal structure of 8ANC195 complexed with fully glycosylated Env trimer, revealing 8ANC195 insertion into a glycan shield gap to contact gp120 and gp41 glycans and protein residues.
26359989	2	53	theme	bNAb	327:330	arg1	structures					313:322	crystal and electron microscopy (EM) structures	276:322	crystal and electron microscopy (EM) structures of bNAb 8ANC195 complexed with monomeric gp120 and trimeric Env, respectively	276:400	The 8ANC195 epitope, defined by crystal and electron microscopy (EM) structures of bNAb 8ANC195 complexed with monomeric gp120 and trimeric Env, respectively, spans the gp120 and gp41 Env subunits.
26359989	6	54	theme	Env	1142:1144	arg1	conformations					1146:1158	different Env conformations	1132:1158	different Env conformations	1132:1158	8ANC195's ability to bind different Env conformations suggests advantages for potential therapeutic applications.
26359989	1	55	theme	neutralizing	199:210	arg1	antibodies					212:221	most neutralizing antibodies	194:221	most neutralizing antibodies	194:221	The HIV-1 envelope (Env) spike contains limited epitopes for broadly neutralizing antibodies (bNAbs); thus, most neutralizing antibodies are strain specific.
26359989	3	56	theme	shield	638:643	arg1	gap					645:647	a glycan shield gap	629:647	a glycan shield gap	629:647	To investigate 8ANC195's gp41 epitope at higher resolution, we solved a 3.58 Å crystal structure of 8ANC195 complexed with fully glycosylated Env trimer, revealing 8ANC195 insertion into a glycan shield gap to contact gp120 and gp41 glycans and protein residues.
26359989	5	57	theme	8ANC195	964:970	arg1	binding					972:978	8ANC195 binding	964:978	8ANC195 binding	964:978	8ANC195 binding partially closed the CD4-bound trimer, confirming structural plasticity of Env by revealing a previously unseen conformation.
26359989	1	58	theme	limited	126:132	arg1	epitopes					134:141	limited epitopes	126:141	limited epitopes for broadly neutralizing antibodies (bNAbs)	126:185	The HIV-1 envelope (Env) spike contains limited epitopes for broadly neutralizing antibodies (bNAbs); thus, most neutralizing antibodies are strain specific.
25329608	10	0	dep	10.4	2081:2084	arg1	determined					2124:2133	determined	2124:2133	were determined using Bragg's relation (d = 2π/q*)	2119:2168	The domain spacing (d) values of the BCC morphology for 2 (ϕMal5 = 0.14) and 3 (ϕMal5 = 0.16) were 10.4 and 9.55 nm, respectively, which were determined using Bragg's relation (d = 2π/q*).
25329608	9	1	theme	amphiphilic	1907:1917	arg1	samples					1923:1929	these Mal5-hybrid amphiphilic BCP samples	1889:1929	these Mal5-hybrid amphiphilic BCP samples	1889:1929	Thus, these Mal5-hybrid amphiphilic BCP samples have a body centered cubic (BCC) phase morphology.
25329608	2	2	theme	N-glycosyl	384:393	arg1	reaction					395:402	the catalyst-free direct N-glycosyl reaction	359:402	the catalyst-free direct N-glycosyl reaction of 5-azidopentylamine onto maltopentaose (Mal5) in dry methanol at room temperature	359:486	The synthesis was started from the catalyst-free direct N-glycosyl reaction of 5-azidopentylamine onto maltopentaose (Mal5) in dry methanol at room temperature and subsequent N-protected reaction with acetic anhydride, producing a stable oligosaccharide-building block, such as Mal5 with an azidopentyl group (Mal5-N3).
25329608	6	3	theme	X-ray	1358:1362	arg1	scattering					1364:1373	The small-angle X-ray scattering	1342:1373	The small-angle X-ray scattering (SAXS) measurements	1342:1393	The small-angle X-ray scattering (SAXS) measurements were accomplished for 2 and 3 to ensure their abilities to form phase separated structures in their bulk states with the increasing temperatures from 30 to 190 °C.
25329608	6	3	theme	X-ray	1358:1362	arg1	SAXS					1376:1379	SAXS	1376:1379	SAXS	1376:1379	The small-angle X-ray scattering (SAXS) measurements were accomplished for 2 and 3 to ensure their abilities to form phase separated structures in their bulk states with the increasing temperatures from 30 to 190 °C.
25329608	1	4	theme	chain	290:294	arg1	polymerization					312:325	the reversible addition-fragmentation chain transfer (RAFT) polymerization	252:325	the reversible addition-fragmentation chain transfer (RAFT) polymerization	252:325	We now describe the synthesis of a new family of oligosaccharide-conjugated functional molecules, which act as chain transfer agents (CTAs) for the reversible addition-fragmentation chain transfer (RAFT) polymerization.
25329608	10	5	theme	BCC	2019:2021	arg1	morphology					2023:2032	the BCC morphology	2015:2032	the BCC morphology	2015:2032	The domain spacing (d) values of the BCC morphology for 2 (ϕMal5 = 0.14) and 3 (ϕMal5 = 0.16) were 10.4 and 9.55 nm, respectively, which were determined using Bragg's relation (d = 2π/q*).
25329608	11	6	theme	polymeric	2259:2267	arg1	materials					2269:2277	the phase separated structural polymeric materials	2228:2277	the phase separated structural polymeric materials in which 5.4 nm bioresource-spherical domains were periodically arrayed at the interval of about 10 nm	2228:2380	The present RAFT agents were shown to eventually provide the phase separated structural polymeric materials in which 5.4 nm bioresource-spherical domains were periodically arrayed at the interval of about 10 nm.
25329608	3	7	theme	aminopentyl	743:753	arg1	Mal5-NH2					762:769	Mal5-NH2	762:769	Mal5-NH2	762:769	The azido group was hydrogenated using platinum dioxide (PtO2) as a catalyst to give Mal5 with aminopentyl group (Mal5-NH2), which was then reacted with CTA molecules bearing activated ester moieties.
25329608	3	7	theme	aminopentyl	743:753	arg1	group					755:759	aminopentyl group	743:759	aminopentyl group (Mal5-NH2)	743:770	The azido group was hydrogenated using platinum dioxide (PtO2) as a catalyst to give Mal5 with aminopentyl group (Mal5-NH2), which was then reacted with CTA molecules bearing activated ester moieties.
25329608	10	8	theme	2π/q	2163:2166	arg1	*					2167:2167	d = 2π/q*	2159:2167	d = 2π/q*	2159:2167	The domain spacing (d) values of the BCC morphology for 2 (ϕMal5 = 0.14) and 3 (ϕMal5 = 0.16) were 10.4 and 9.55 nm, respectively, which were determined using Bragg's relation (d = 2π/q*).
25329608	10	8	theme	2π/q	2163:2166	arg1	relation					2149:2156	Bragg's relation	2141:2156	Bragg's relation (d = 2π/q*)	2141:2168	The domain spacing (d) values of the BCC morphology for 2 (ϕMal5 = 0.14) and 3 (ϕMal5 = 0.16) were 10.4 and 9.55 nm, respectively, which were determined using Bragg's relation (d = 2π/q*).
25329608	4	9	theme	Mal5-modified	874:886	arg1	macro-CTAs					888:897	Mal5-modified macro-CTAs	874:897	Mal5-modified macro-CTAs (Mal5-CTAs, 1)	874:912	These reactions produced Mal5-modified macro-CTAs (Mal5-CTAs, 1), which were used for the RAFT polymerizations of styrene (St) and methyl methacrylate (MMA) in DMF.
25329608	7	10	theme	ϕMal5	1622:1626	arg1	2					1598:1598	2	1598:1598	2	1598:1598	The featured results were observed for 2 (ϕMal5 = 0.14) and 3 (ϕMal5 = 0.16) at temperatures above 100 °C, where ϕMal5 denotes the volume fraction of the Mal5 unit in the BCP sample.
25329608	7	10	theme	ϕMal5	1622:1626	arg1	0.16					1630:1633	ϕMal5 = 0.16	1622:1633	ϕMal5 = 0.16	1622:1633	The featured results were observed for 2 (ϕMal5 = 0.14) and 3 (ϕMal5 = 0.16) at temperatures above 100 °C, where ϕMal5 denotes the volume fraction of the Mal5 unit in the BCP sample.
25329608	10	11	theme	=	2068:2068	arg1	2					2038:2038	2	2038:2038	2	2038:2038	The domain spacing (d) values of the BCC morphology for 2 (ϕMal5 = 0.14) and 3 (ϕMal5 = 0.16) were 10.4 and 9.55 nm, respectively, which were determined using Bragg's relation (d = 2π/q*).
25329608	10	11	theme	=	2068:2068	arg1	0.16					2070:2073	ϕMal5 = 0.16	2062:2073	ϕMal5 = 0.16	2062:2073	The domain spacing (d) values of the BCC morphology for 2 (ϕMal5 = 0.14) and 3 (ϕMal5 = 0.16) were 10.4 and 9.55 nm, respectively, which were determined using Bragg's relation (d = 2π/q*).
25329608	9	12	theme	centered	1943:1950	arg1	morphology					1970:1979	a body centered cubic (BCC) phase morphology	1936:1979	a body centered cubic (BCC) phase morphology	1936:1979	Thus, these Mal5-hybrid amphiphilic BCP samples have a body centered cubic (BCC) phase morphology.
25329608	1	13	theme	chain	219:223	arg1	agents					234:239	chain transfer agents	219:239	chain transfer agents (CTAs) for the reversible addition-fragmentation chain transfer (RAFT) polymerization	219:325	We now describe the synthesis of a new family of oligosaccharide-conjugated functional molecules, which act as chain transfer agents (CTAs) for the reversible addition-fragmentation chain transfer (RAFT) polymerization.
25329608	1	13	theme	chain	219:223	arg1	molecules					195:203	oligosaccharide-conjugated functional molecules	157:203	oligosaccharide-conjugated functional molecules	157:203	We now describe the synthesis of a new family of oligosaccharide-conjugated functional molecules, which act as chain transfer agents (CTAs) for the reversible addition-fragmentation chain transfer (RAFT) polymerization.
25329608	1	13	theme	chain	219:223	arg1	CTAs					242:245	CTAs	242:245	CTAs	242:245	We now describe the synthesis of a new family of oligosaccharide-conjugated functional molecules, which act as chain transfer agents (CTAs) for the reversible addition-fragmentation chain transfer (RAFT) polymerization.
25329608	8	14	dep	peaks	1862:1866	arg1	*					1871:1871	√2q*	1868:1871	√2q*	1868:1871	For both BCP samples, the primary scattering peaks q* were clearly observed together with the higher-ordered scattering peaks √2q* and √3q*.
25329608	8	14	dep	peaks	1862:1866	arg1	*					1880:1880	√3q*	1877:1880	√3q*	1877:1880	For both BCP samples, the primary scattering peaks q* were clearly observed together with the higher-ordered scattering peaks √2q* and √3q*.
25329608	5	15	theme	[M	1059:1060	arg1	0/[1					1062:1065	the [M]0/[1]0 values ranging from 50 to 600, affording the Mal5-hybrid amphiphilic block copolymers (BCPs), such as Mal5-polystyrene (2) and Mal5-poly(methyl methacrylate) (3), with a quantitative end-functionality and the controlled molecular weights between 4310 and 20 300 g mol(-1)	1055:1339	the [M]0/[1]0 values ranging from 50 to 600, affording the Mal5-hybrid amphiphilic block copolymers (BCPs), such as Mal5-polystyrene (2) and Mal5-poly(methyl methacrylate) (3), with a quantitative end-functionality and the controlled molecular weights between 4310 and 20 300 g mol(-1)	1055:1339	The polymerizations were performed using the [M]0/[1]0 values ranging from 50 to 600, affording the Mal5-hybrid amphiphilic block copolymers (BCPs), such as Mal5-polystyrene (2) and Mal5-poly(methyl methacrylate) (3), with a quantitative end-functionality and the controlled molecular weights between 4310 and 20 300 g mol(-1).
25329608	11	16	theme	RAFT	2183:2186	arg1	agents					2188:2193	The present RAFT agents	2171:2193	The present RAFT agents	2171:2193	The present RAFT agents were shown to eventually provide the phase separated structural polymeric materials in which 5.4 nm bioresource-spherical domains were periodically arrayed at the interval of about 10 nm.
25329608	9	17	theme	BCP	1919:1921	arg1	samples					1923:1929	these Mal5-hybrid amphiphilic BCP samples	1889:1929	these Mal5-hybrid amphiphilic BCP samples	1889:1929	Thus, these Mal5-hybrid amphiphilic BCP samples have a body centered cubic (BCC) phase morphology.
25329608	1	18	theme	new	143:145	arg1	family					147:152	a new family	141:152	a new family of oligosaccharide-conjugated functional molecules, which act as chain transfer agents (CTAs) for the reversible addition-fragmentation chain transfer (RAFT) polymerization	141:325	We now describe the synthesis of a new family of oligosaccharide-conjugated functional molecules, which act as chain transfer agents (CTAs) for the reversible addition-fragmentation chain transfer (RAFT) polymerization.
25329608	10	19	theme	ϕMal5	2062:2066	arg1	2					2038:2038	2	2038:2038	2	2038:2038	The domain spacing (d) values of the BCC morphology for 2 (ϕMal5 = 0.14) and 3 (ϕMal5 = 0.16) were 10.4 and 9.55 nm, respectively, which were determined using Bragg's relation (d = 2π/q*).
25329608	10	19	theme	ϕMal5	2062:2066	arg1	0.16					2070:2073	ϕMal5 = 0.16	2062:2073	ϕMal5 = 0.16	2062:2073	The domain spacing (d) values of the BCC morphology for 2 (ϕMal5 = 0.14) and 3 (ϕMal5 = 0.16) were 10.4 and 9.55 nm, respectively, which were determined using Bragg's relation (d = 2π/q*).
25329608	5	20	theme	methyl	1206:1211	arg1	Mal5-poly					1196:1204	Mal5-poly	1196:1204	Mal5-poly(methyl methacrylate) (3)	1196:1229	The polymerizations were performed using the [M]0/[1]0 values ranging from 50 to 600, affording the Mal5-hybrid amphiphilic block copolymers (BCPs), such as Mal5-polystyrene (2) and Mal5-poly(methyl methacrylate) (3), with a quantitative end-functionality and the controlled molecular weights between 4310 and 20 300 g mol(-1).
25329608	5	20	theme	methyl	1206:1211	arg1	methacrylate					1213:1224	methyl methacrylate	1206:1224	methyl methacrylate	1206:1224	The polymerizations were performed using the [M]0/[1]0 values ranging from 50 to 600, affording the Mal5-hybrid amphiphilic block copolymers (BCPs), such as Mal5-polystyrene (2) and Mal5-poly(methyl methacrylate) (3), with a quantitative end-functionality and the controlled molecular weights between 4310 and 20 300 g mol(-1).
25329608	8	21	theme	BCP	1751:1753	arg1	samples					1755:1761	both BCP samples	1746:1761	both BCP samples	1746:1761	For both BCP samples, the primary scattering peaks q* were clearly observed together with the higher-ordered scattering peaks √2q* and √3q*.
25329608	6	22	theme	phase	1459:1463	arg1	structures					1475:1484	phase separated structures	1459:1484	phase separated structures in their bulk states	1459:1505	The small-angle X-ray scattering (SAXS) measurements were accomplished for 2 and 3 to ensure their abilities to form phase separated structures in their bulk states with the increasing temperatures from 30 to 190 °C.
25329608	5	23	with	weights	1299:1305	arg1	end-functionality					1252:1268	a quantitative end-functionality	1237:1268	a quantitative end-functionality	1237:1268	The polymerizations were performed using the [M]0/[1]0 values ranging from 50 to 600, affording the Mal5-hybrid amphiphilic block copolymers (BCPs), such as Mal5-polystyrene (2) and Mal5-poly(methyl methacrylate) (3), with a quantitative end-functionality and the controlled molecular weights between 4310 and 20 300 g mol(-1).
25329608	3	24	theme	azido	652:656	arg1	group					658:662	The azido group	648:662	The azido group	648:662	The azido group was hydrogenated using platinum dioxide (PtO2) as a catalyst to give Mal5 with aminopentyl group (Mal5-NH2), which was then reacted with CTA molecules bearing activated ester moieties.
25329608	1	25	theme	functional	184:193	arg1	agents					234:239	chain transfer agents	219:239	chain transfer agents (CTAs) for the reversible addition-fragmentation chain transfer (RAFT) polymerization	219:325	We now describe the synthesis of a new family of oligosaccharide-conjugated functional molecules, which act as chain transfer agents (CTAs) for the reversible addition-fragmentation chain transfer (RAFT) polymerization.
25329608	1	25	theme	functional	184:193	arg1	molecules					195:203	oligosaccharide-conjugated functional molecules	157:203	oligosaccharide-conjugated functional molecules	157:203	We now describe the synthesis of a new family of oligosaccharide-conjugated functional molecules, which act as chain transfer agents (CTAs) for the reversible addition-fragmentation chain transfer (RAFT) polymerization.
25329608	8	26	theme	primary	1768:1774	arg1	peaks					1787:1791	the primary scattering peaks	1764:1791	the primary scattering peaks q*	1764:1794	For both BCP samples, the primary scattering peaks q* were clearly observed together with the higher-ordered scattering peaks √2q* and √3q*.
25329608	8	27	dep	peaks	1787:1791	arg1	*					1794:1794	q*	1793:1794	the primary scattering peaks q*	1764:1794	For both BCP samples, the primary scattering peaks q* were clearly observed together with the higher-ordered scattering peaks √2q* and √3q*.
25329608	2	28	from	temperature	476:486	arg1	reaction					395:402	the catalyst-free direct N-glycosyl reaction	359:402	the catalyst-free direct N-glycosyl reaction of 5-azidopentylamine onto maltopentaose (Mal5) in dry methanol at room temperature	359:486	The synthesis was started from the catalyst-free direct N-glycosyl reaction of 5-azidopentylamine onto maltopentaose (Mal5) in dry methanol at room temperature and subsequent N-protected reaction with acetic anhydride, producing a stable oligosaccharide-building block, such as Mal5 with an azidopentyl group (Mal5-N3).
25329608	2	28	from	temperature	476:486	arg1	reaction					515:522	subsequent N-protected reaction	492:522	subsequent N-protected reaction with acetic anhydride, producing a stable oligosaccharide-building block, such as Mal5 with an azidopentyl group (Mal5-N3)	492:645	The synthesis was started from the catalyst-free direct N-glycosyl reaction of 5-azidopentylamine onto maltopentaose (Mal5) in dry methanol at room temperature and subsequent N-protected reaction with acetic anhydride, producing a stable oligosaccharide-building block, such as Mal5 with an azidopentyl group (Mal5-N3).
25329608	2	28	from	temperature	476:486	arg1	methanol					459:466	dry methanol	455:466	dry methanol at room temperature	455:486	The synthesis was started from the catalyst-free direct N-glycosyl reaction of 5-azidopentylamine onto maltopentaose (Mal5) in dry methanol at room temperature and subsequent N-protected reaction with acetic anhydride, producing a stable oligosaccharide-building block, such as Mal5 with an azidopentyl group (Mal5-N3).
25329608	10	29	theme	spacing	1993:1999	arg1	10.4					2081:2084	10.4	2081:2084	10.4	2081:2084	The domain spacing (d) values of the BCC morphology for 2 (ϕMal5 = 0.14) and 3 (ϕMal5 = 0.16) were 10.4 and 9.55 nm, respectively, which were determined using Bragg's relation (d = 2π/q*).
25329608	10	29	theme	spacing	1993:1999	arg1	values					2005:2010	The domain spacing (d) values	1982:2010	The domain spacing (d) values of the BCC morphology for 2 (ϕMal5 = 0.14) and 3 (ϕMal5 = 0.16)	1982:2074	The domain spacing (d) values of the BCC morphology for 2 (ϕMal5 = 0.14) and 3 (ϕMal5 = 0.16) were 10.4 and 9.55 nm, respectively, which were determined using Bragg's relation (d = 2π/q*).
25329608	3	30	theme	activated	823:831	arg1	moieties					839:846	activated ester moieties	823:846	activated ester moieties	823:846	The azido group was hydrogenated using platinum dioxide (PtO2) as a catalyst to give Mal5 with aminopentyl group (Mal5-NH2), which was then reacted with CTA molecules bearing activated ester moieties.
25329608	2	31	theme	stable	559:564	arg1	block					591:595	a stable oligosaccharide-building block	557:595	a stable oligosaccharide-building block	557:595	The synthesis was started from the catalyst-free direct N-glycosyl reaction of 5-azidopentylamine onto maltopentaose (Mal5) in dry methanol at room temperature and subsequent N-protected reaction with acetic anhydride, producing a stable oligosaccharide-building block, such as Mal5 with an azidopentyl group (Mal5-N3).
25329608	8	32	theme	√2q	1868:1870	arg1	*					1871:1871	√2q*	1868:1871	√2q*	1868:1871	For both BCP samples, the primary scattering peaks q* were clearly observed together with the higher-ordered scattering peaks √2q* and √3q*.
25329608	2	33	from	reaction	515:522	arg1	temperature					476:486	room temperature	471:486	room temperature	471:486	The synthesis was started from the catalyst-free direct N-glycosyl reaction of 5-azidopentylamine onto maltopentaose (Mal5) in dry methanol at room temperature and subsequent N-protected reaction with acetic anhydride, producing a stable oligosaccharide-building block, such as Mal5 with an azidopentyl group (Mal5-N3).
25329608	2	33	from	reaction	515:522	arg1	methanol					459:466	dry methanol	455:466	dry methanol at room temperature	455:486	The synthesis was started from the catalyst-free direct N-glycosyl reaction of 5-azidopentylamine onto maltopentaose (Mal5) in dry methanol at room temperature and subsequent N-protected reaction with acetic anhydride, producing a stable oligosaccharide-building block, such as Mal5 with an azidopentyl group (Mal5-N3).
25329608	0	34	theme	Maltopentaose-conjugated	0:23	arg1	CTA					25:27	Maltopentaose-conjugated CTA	0:27	Maltopentaose-conjugated CTA for RAFT polymerization	0:51	Maltopentaose-conjugated CTA for RAFT polymerization generating nanostructured bioresource-block copolymer.
25329608	1	35	theme	RAFT	306:309	arg1	polymerization					312:325	the reversible addition-fragmentation chain transfer (RAFT) polymerization	252:325	the reversible addition-fragmentation chain transfer (RAFT) polymerization	252:325	We now describe the synthesis of a new family of oligosaccharide-conjugated functional molecules, which act as chain transfer agents (CTAs) for the reversible addition-fragmentation chain transfer (RAFT) polymerization.
25329608	7	36	theme	BCP	1730:1732	arg1	sample					1734:1739	the BCP sample	1726:1739	the BCP sample	1726:1739	The featured results were observed for 2 (ϕMal5 = 0.14) and 3 (ϕMal5 = 0.16) at temperatures above 100 °C, where ϕMal5 denotes the volume fraction of the Mal5 unit in the BCP sample.
25329608	2	37	theme	room	471:474	arg1	temperature					476:486	room temperature	471:486	room temperature	471:486	The synthesis was started from the catalyst-free direct N-glycosyl reaction of 5-azidopentylamine onto maltopentaose (Mal5) in dry methanol at room temperature and subsequent N-protected reaction with acetic anhydride, producing a stable oligosaccharide-building block, such as Mal5 with an azidopentyl group (Mal5-N3).
25329608	10	38	theme	d	2002:2002	arg1	10.4					2081:2084	10.4	2081:2084	10.4	2081:2084	The domain spacing (d) values of the BCC morphology for 2 (ϕMal5 = 0.14) and 3 (ϕMal5 = 0.16) were 10.4 and 9.55 nm, respectively, which were determined using Bragg's relation (d = 2π/q*).
25329608	10	38	theme	d	2002:2002	arg1	values					2005:2010	The domain spacing (d) values	1982:2010	The domain spacing (d) values of the BCC morphology for 2 (ϕMal5 = 0.14) and 3 (ϕMal5 = 0.16)	1982:2074	The domain spacing (d) values of the BCC morphology for 2 (ϕMal5 = 0.14) and 3 (ϕMal5 = 0.16) were 10.4 and 9.55 nm, respectively, which were determined using Bragg's relation (d = 2π/q*).
25329608	5	39	theme	block	1138:1142	arg1	Mal5-poly					1196:1204	Mal5-poly	1196:1204	Mal5-poly(methyl methacrylate) (3)	1196:1229	The polymerizations were performed using the [M]0/[1]0 values ranging from 50 to 600, affording the Mal5-hybrid amphiphilic block copolymers (BCPs), such as Mal5-polystyrene (2) and Mal5-poly(methyl methacrylate) (3), with a quantitative end-functionality and the controlled molecular weights between 4310 and 20 300 g mol(-1).
25329608	5	39	theme	block	1138:1142	arg1	Mal5-polystyrene					1171:1186	Mal5-polystyrene (2)	1171:1190	Mal5-polystyrene (2)	1171:1190	The polymerizations were performed using the [M]0/[1]0 values ranging from 50 to 600, affording the Mal5-hybrid amphiphilic block copolymers (BCPs), such as Mal5-polystyrene (2) and Mal5-poly(methyl methacrylate) (3), with a quantitative end-functionality and the controlled molecular weights between 4310 and 20 300 g mol(-1).
25329608	5	39	theme	block	1138:1142	arg1	BCPs					1156:1159	BCPs	1156:1159	BCPs	1156:1159	The polymerizations were performed using the [M]0/[1]0 values ranging from 50 to 600, affording the Mal5-hybrid amphiphilic block copolymers (BCPs), such as Mal5-polystyrene (2) and Mal5-poly(methyl methacrylate) (3), with a quantitative end-functionality and the controlled molecular weights between 4310 and 20 300 g mol(-1).
25329608	5	39	theme	block	1138:1142	arg1	copolymers					1144:1153	the Mal5-hybrid amphiphilic block copolymers	1110:1153	the Mal5-hybrid amphiphilic block copolymers (BCPs)	1110:1160	The polymerizations were performed using the [M]0/[1]0 values ranging from 50 to 600, affording the Mal5-hybrid amphiphilic block copolymers (BCPs), such as Mal5-polystyrene (2) and Mal5-poly(methyl methacrylate) (3), with a quantitative end-functionality and the controlled molecular weights between 4310 and 20 300 g mol(-1).
25329608	4	40	theme	methacrylate	987:998	arg1	polymerizations					944:958	the RAFT polymerizations	935:958	the RAFT polymerizations of styrene (St) and methyl methacrylate (MMA)	935:1004	These reactions produced Mal5-modified macro-CTAs (Mal5-CTAs, 1), which were used for the RAFT polymerizations of styrene (St) and methyl methacrylate (MMA) in DMF.
25329608	11	41	theme	nm	2292:2293	arg1	domains					2317:2323	5.4 nm bioresource-spherical domains	2288:2323	5.4 nm bioresource-spherical domains	2288:2323	The present RAFT agents were shown to eventually provide the phase separated structural polymeric materials in which 5.4 nm bioresource-spherical domains were periodically arrayed at the interval of about 10 nm.
25329608	7	42	theme	Mal5	1713:1716	arg1	unit					1718:1721	the Mal5 unit	1709:1721	the Mal5 unit	1709:1721	The featured results were observed for 2 (ϕMal5 = 0.14) and 3 (ϕMal5 = 0.16) at temperatures above 100 °C, where ϕMal5 denotes the volume fraction of the Mal5 unit in the BCP sample.
25329608	2	43	theme	N-protected	503:513	arg1	reaction					515:522	subsequent N-protected reaction	492:522	subsequent N-protected reaction with acetic anhydride, producing a stable oligosaccharide-building block, such as Mal5 with an azidopentyl group (Mal5-N3)	492:645	The synthesis was started from the catalyst-free direct N-glycosyl reaction of 5-azidopentylamine onto maltopentaose (Mal5) in dry methanol at room temperature and subsequent N-protected reaction with acetic anhydride, producing a stable oligosaccharide-building block, such as Mal5 with an azidopentyl group (Mal5-N3).
25329608	2	44	theme	direct	377:382	arg1	reaction					395:402	the catalyst-free direct N-glycosyl reaction	359:402	the catalyst-free direct N-glycosyl reaction of 5-azidopentylamine onto maltopentaose (Mal5) in dry methanol at room temperature	359:486	The synthesis was started from the catalyst-free direct N-glycosyl reaction of 5-azidopentylamine onto maltopentaose (Mal5) in dry methanol at room temperature and subsequent N-protected reaction with acetic anhydride, producing a stable oligosaccharide-building block, such as Mal5 with an azidopentyl group (Mal5-N3).
25329608	0	45	theme	nanostructured	64:77	arg1	copolymer					97:105	nanostructured bioresource-block copolymer	64:105	nanostructured bioresource-block copolymer	64:105	Maltopentaose-conjugated CTA for RAFT polymerization generating nanostructured bioresource-block copolymer.
25329608	2	46	with	reaction	395:402	arg1	anhydride					536:544	acetic anhydride	529:544	acetic anhydride	529:544	The synthesis was started from the catalyst-free direct N-glycosyl reaction of 5-azidopentylamine onto maltopentaose (Mal5) in dry methanol at room temperature and subsequent N-protected reaction with acetic anhydride, producing a stable oligosaccharide-building block, such as Mal5 with an azidopentyl group (Mal5-N3).
25329608	7	47	theme	volume	1690:1695	arg1	fraction					1697:1704	the volume fraction	1686:1704	the volume fraction of the Mal5 unit	1686:1721	The featured results were observed for 2 (ϕMal5 = 0.14) and 3 (ϕMal5 = 0.16) at temperatures above 100 °C, where ϕMal5 denotes the volume fraction of the Mal5 unit in the BCP sample.
25329608	11	48	theme	structural	2248:2257	arg1	materials					2269:2277	the phase separated structural polymeric materials	2228:2277	the phase separated structural polymeric materials in which 5.4 nm bioresource-spherical domains were periodically arrayed at the interval of about 10 nm	2228:2380	The present RAFT agents were shown to eventually provide the phase separated structural polymeric materials in which 5.4 nm bioresource-spherical domains were periodically arrayed at the interval of about 10 nm.
25329608	2	49	theme	5-azidopentylamine	407:424	arg1	reaction					395:402	the catalyst-free direct N-glycosyl reaction	359:402	the catalyst-free direct N-glycosyl reaction of 5-azidopentylamine onto maltopentaose (Mal5) in dry methanol at room temperature	359:486	The synthesis was started from the catalyst-free direct N-glycosyl reaction of 5-azidopentylamine onto maltopentaose (Mal5) in dry methanol at room temperature and subsequent N-protected reaction with acetic anhydride, producing a stable oligosaccharide-building block, such as Mal5 with an azidopentyl group (Mal5-N3).
25329608	2	49	theme	5-azidopentylamine	407:424	arg1	reaction					515:522	subsequent N-protected reaction	492:522	subsequent N-protected reaction with acetic anhydride, producing a stable oligosaccharide-building block, such as Mal5 with an azidopentyl group (Mal5-N3)	492:645	The synthesis was started from the catalyst-free direct N-glycosyl reaction of 5-azidopentylamine onto maltopentaose (Mal5) in dry methanol at room temperature and subsequent N-protected reaction with acetic anhydride, producing a stable oligosaccharide-building block, such as Mal5 with an azidopentyl group (Mal5-N3).
25329608	3	50	theme	CTA	801:803	arg1	molecules					805:813	CTA molecules	801:813	CTA molecules bearing activated ester moieties	801:846	The azido group was hydrogenated using platinum dioxide (PtO2) as a catalyst to give Mal5 with aminopentyl group (Mal5-NH2), which was then reacted with CTA molecules bearing activated ester moieties.
25329608	11	51	theme	phase	2232:2236	arg1	materials					2269:2277	the phase separated structural polymeric materials	2228:2277	the phase separated structural polymeric materials in which 5.4 nm bioresource-spherical domains were periodically arrayed at the interval of about 10 nm	2228:2380	The present RAFT agents were shown to eventually provide the phase separated structural polymeric materials in which 5.4 nm bioresource-spherical domains were periodically arrayed at the interval of about 10 nm.
25329608	9	52	theme	Mal5-hybrid	1895:1905	arg1	samples					1923:1929	these Mal5-hybrid amphiphilic BCP samples	1889:1929	these Mal5-hybrid amphiphilic BCP samples	1889:1929	Thus, these Mal5-hybrid amphiphilic BCP samples have a body centered cubic (BCC) phase morphology.
25329608	5	53	theme	molecular	1289:1297	arg1	Mal5-poly					1196:1204	Mal5-poly	1196:1204	Mal5-poly(methyl methacrylate) (3)	1196:1229	The polymerizations were performed using the [M]0/[1]0 values ranging from 50 to 600, affording the Mal5-hybrid amphiphilic block copolymers (BCPs), such as Mal5-polystyrene (2) and Mal5-poly(methyl methacrylate) (3), with a quantitative end-functionality and the controlled molecular weights between 4310 and 20 300 g mol(-1).
25329608	5	53	theme	molecular	1289:1297	arg1	Mal5-polystyrene					1171:1186	Mal5-polystyrene (2)	1171:1190	Mal5-polystyrene (2)	1171:1190	The polymerizations were performed using the [M]0/[1]0 values ranging from 50 to 600, affording the Mal5-hybrid amphiphilic block copolymers (BCPs), such as Mal5-polystyrene (2) and Mal5-poly(methyl methacrylate) (3), with a quantitative end-functionality and the controlled molecular weights between 4310 and 20 300 g mol(-1).
25329608	5	53	theme	molecular	1289:1297	arg1	weights					1299:1305	the controlled molecular weights	1274:1305	the controlled molecular weights between 4310 and 20 300 g mol(-1)	1274:1339	The polymerizations were performed using the [M]0/[1]0 values ranging from 50 to 600, affording the Mal5-hybrid amphiphilic block copolymers (BCPs), such as Mal5-polystyrene (2) and Mal5-poly(methyl methacrylate) (3), with a quantitative end-functionality and the controlled molecular weights between 4310 and 20 300 g mol(-1).
25329608	6	54	theme	scattering	1364:1373	arg1	measurements					1382:1393	The small-angle X-ray scattering (SAXS) measurements	1342:1393	The small-angle X-ray scattering (SAXS) measurements	1342:1393	The small-angle X-ray scattering (SAXS) measurements were accomplished for 2 and 3 to ensure their abilities to form phase separated structures in their bulk states with the increasing temperatures from 30 to 190 °C.
25329608	1	55	theme	addition-fragmentation	267:288	arg1	polymerization					312:325	the reversible addition-fragmentation chain transfer (RAFT) polymerization	252:325	the reversible addition-fragmentation chain transfer (RAFT) polymerization	252:325	We now describe the synthesis of a new family of oligosaccharide-conjugated functional molecules, which act as chain transfer agents (CTAs) for the reversible addition-fragmentation chain transfer (RAFT) polymerization.
25329608	10	56	theme	morphology	2023:2032	arg1	10.4					2081:2084	10.4	2081:2084	10.4	2081:2084	The domain spacing (d) values of the BCC morphology for 2 (ϕMal5 = 0.14) and 3 (ϕMal5 = 0.16) were 10.4 and 9.55 nm, respectively, which were determined using Bragg's relation (d = 2π/q*).
25329608	10	56	theme	morphology	2023:2032	arg1	values					2005:2010	The domain spacing (d) values	1982:2010	The domain spacing (d) values of the BCC morphology for 2 (ϕMal5 = 0.14) and 3 (ϕMal5 = 0.16)	1982:2074	The domain spacing (d) values of the BCC morphology for 2 (ϕMal5 = 0.14) and 3 (ϕMal5 = 0.16) were 10.4 and 9.55 nm, respectively, which were determined using Bragg's relation (d = 2π/q*).
25329608	5	57	with	copolymers	1144:1153	arg1	end-functionality					1252:1268	a quantitative end-functionality	1237:1268	a quantitative end-functionality	1237:1268	The polymerizations were performed using the [M]0/[1]0 values ranging from 50 to 600, affording the Mal5-hybrid amphiphilic block copolymers (BCPs), such as Mal5-polystyrene (2) and Mal5-poly(methyl methacrylate) (3), with a quantitative end-functionality and the controlled molecular weights between 4310 and 20 300 g mol(-1).
25329608	6	58	theme	small-angle	1346:1356	arg1	scattering					1364:1373	The small-angle X-ray scattering	1342:1373	The small-angle X-ray scattering (SAXS) measurements	1342:1393	The small-angle X-ray scattering (SAXS) measurements were accomplished for 2 and 3 to ensure their abilities to form phase separated structures in their bulk states with the increasing temperatures from 30 to 190 °C.
25329608	6	58	theme	small-angle	1346:1356	arg1	SAXS					1376:1379	SAXS	1376:1379	SAXS	1376:1379	The small-angle X-ray scattering (SAXS) measurements were accomplished for 2 and 3 to ensure their abilities to form phase separated structures in their bulk states with the increasing temperatures from 30 to 190 °C.
25329608	1	59	theme	transfer	296:303	arg1	polymerization					312:325	the reversible addition-fragmentation chain transfer (RAFT) polymerization	252:325	the reversible addition-fragmentation chain transfer (RAFT) polymerization	252:325	We now describe the synthesis of a new family of oligosaccharide-conjugated functional molecules, which act as chain transfer agents (CTAs) for the reversible addition-fragmentation chain transfer (RAFT) polymerization.
25329608	7	60	located	observed	1585:1592	arg1	temperatures					1639:1650	temperatures	1639:1650	temperatures above 100 °C, where ϕMal5 denotes the volume fraction of the Mal5 unit in the BCP sample	1639:1739	The featured results were observed for 2 (ϕMal5 = 0.14) and 3 (ϕMal5 = 0.16) at temperatures above 100 °C, where ϕMal5 denotes the volume fraction of the Mal5 unit in the BCP sample.
25329608	7	60	located	observed	1585:1592	arg2	results					1572:1578	The featured results	1559:1578	The featured results	1559:1578	The featured results were observed for 2 (ϕMal5 = 0.14) and 3 (ϕMal5 = 0.16) at temperatures above 100 °C, where ϕMal5 denotes the volume fraction of the Mal5 unit in the BCP sample.
25329608	7	61	theme	=	1628:1628	arg1	2					1598:1598	2	1598:1598	2	1598:1598	The featured results were observed for 2 (ϕMal5 = 0.14) and 3 (ϕMal5 = 0.16) at temperatures above 100 °C, where ϕMal5 denotes the volume fraction of the Mal5 unit in the BCP sample.
25329608	7	61	theme	=	1628:1628	arg1	0.16					1630:1633	ϕMal5 = 0.16	1622:1633	ϕMal5 = 0.16	1622:1633	The featured results were observed for 2 (ϕMal5 = 0.14) and 3 (ϕMal5 = 0.16) at temperatures above 100 °C, where ϕMal5 denotes the volume fraction of the Mal5 unit in the BCP sample.
25329608	8	62	theme	scattering	1851:1860	arg1	peaks					1862:1866	the higher-ordered scattering peaks	1832:1866	the higher-ordered scattering peaks √2q* and √3q*	1832:1880	For both BCP samples, the primary scattering peaks q* were clearly observed together with the higher-ordered scattering peaks √2q* and √3q*.
25329608	2	63	from	reaction	395:402	arg1	temperature					476:486	room temperature	471:486	room temperature	471:486	The synthesis was started from the catalyst-free direct N-glycosyl reaction of 5-azidopentylamine onto maltopentaose (Mal5) in dry methanol at room temperature and subsequent N-protected reaction with acetic anhydride, producing a stable oligosaccharide-building block, such as Mal5 with an azidopentyl group (Mal5-N3).
25329608	2	63	from	reaction	395:402	arg1	methanol					459:466	dry methanol	455:466	dry methanol at room temperature	455:486	The synthesis was started from the catalyst-free direct N-glycosyl reaction of 5-azidopentylamine onto maltopentaose (Mal5) in dry methanol at room temperature and subsequent N-protected reaction with acetic anhydride, producing a stable oligosaccharide-building block, such as Mal5 with an azidopentyl group (Mal5-N3).
25329608	10	64	theme	=	2161:2161	arg1	*					2167:2167	d = 2π/q*	2159:2167	d = 2π/q*	2159:2167	The domain spacing (d) values of the BCC morphology for 2 (ϕMal5 = 0.14) and 3 (ϕMal5 = 0.16) were 10.4 and 9.55 nm, respectively, which were determined using Bragg's relation (d = 2π/q*).
25329608	10	64	theme	=	2161:2161	arg1	relation					2149:2156	Bragg's relation	2141:2156	Bragg's relation (d = 2π/q*)	2141:2168	The domain spacing (d) values of the BCC morphology for 2 (ϕMal5 = 0.14) and 3 (ϕMal5 = 0.16) were 10.4 and 9.55 nm, respectively, which were determined using Bragg's relation (d = 2π/q*).
25329608	9	65	theme	body	1938:1941	arg1	morphology					1970:1979	a body centered cubic (BCC) phase morphology	1936:1979	a body centered cubic (BCC) phase morphology	1936:1979	Thus, these Mal5-hybrid amphiphilic BCP samples have a body centered cubic (BCC) phase morphology.
25329608	5	66	theme	g	1331:1331	arg1	mol					1333:1335	20 300 g mol	1324:1335	20 300 g mol(-1)	1324:1339	The polymerizations were performed using the [M]0/[1]0 values ranging from 50 to 600, affording the Mal5-hybrid amphiphilic block copolymers (BCPs), such as Mal5-polystyrene (2) and Mal5-poly(methyl methacrylate) (3), with a quantitative end-functionality and the controlled molecular weights between 4310 and 20 300 g mol(-1).
25329608	5	66	theme	g	1331:1331	arg1	-1					1337:1338	-1	1337:1338	-1	1337:1338	The polymerizations were performed using the [M]0/[1]0 values ranging from 50 to 600, affording the Mal5-hybrid amphiphilic block copolymers (BCPs), such as Mal5-polystyrene (2) and Mal5-poly(methyl methacrylate) (3), with a quantitative end-functionality and the controlled molecular weights between 4310 and 20 300 g mol(-1).
25329608	6	67	dep	190	1551:1553	arg1	to					1548:1549	to	1548:1549	to	1548:1549	The small-angle X-ray scattering (SAXS) measurements were accomplished for 2 and 3 to ensure their abilities to form phase separated structures in their bulk states with the increasing temperatures from 30 to 190 °C.
25329608	9	68	theme	cubic	1952:1956	arg1	morphology					1970:1979	a body centered cubic (BCC) phase morphology	1936:1979	a body centered cubic (BCC) phase morphology	1936:1979	Thus, these Mal5-hybrid amphiphilic BCP samples have a body centered cubic (BCC) phase morphology.
25329608	4	69	dep	styrene	963:969	arg1	St					972:973	St	972:973	St	972:973	These reactions produced Mal5-modified macro-CTAs (Mal5-CTAs, 1), which were used for the RAFT polymerizations of styrene (St) and methyl methacrylate (MMA) in DMF.
25329608	1	70	theme	transfer	225:232	arg1	agents					234:239	chain transfer agents	219:239	chain transfer agents (CTAs) for the reversible addition-fragmentation chain transfer (RAFT) polymerization	219:325	We now describe the synthesis of a new family of oligosaccharide-conjugated functional molecules, which act as chain transfer agents (CTAs) for the reversible addition-fragmentation chain transfer (RAFT) polymerization.
25329608	1	70	theme	transfer	225:232	arg1	molecules					195:203	oligosaccharide-conjugated functional molecules	157:203	oligosaccharide-conjugated functional molecules	157:203	We now describe the synthesis of a new family of oligosaccharide-conjugated functional molecules, which act as chain transfer agents (CTAs) for the reversible addition-fragmentation chain transfer (RAFT) polymerization.
25329608	1	70	theme	transfer	225:232	arg1	CTAs					242:245	CTAs	242:245	CTAs	242:245	We now describe the synthesis of a new family of oligosaccharide-conjugated functional molecules, which act as chain transfer agents (CTAs) for the reversible addition-fragmentation chain transfer (RAFT) polymerization.
25329608	9	71	contain	have	1931:1934	arg2	morphology					1970:1979	a body centered cubic (BCC) phase morphology	1936:1979	a body centered cubic (BCC) phase morphology	1936:1979	Thus, these Mal5-hybrid amphiphilic BCP samples have a body centered cubic (BCC) phase morphology.
25329608	9	71	contain	have	1931:1934	arg1	samples					1923:1929	these Mal5-hybrid amphiphilic BCP samples	1889:1929	these Mal5-hybrid amphiphilic BCP samples	1889:1929	Thus, these Mal5-hybrid amphiphilic BCP samples have a body centered cubic (BCC) phase morphology.
25329608	9	72	theme	BCC	1959:1961	arg1	morphology					1970:1979	a body centered cubic (BCC) phase morphology	1936:1979	a body centered cubic (BCC) phase morphology	1936:1979	Thus, these Mal5-hybrid amphiphilic BCP samples have a body centered cubic (BCC) phase morphology.
25329608	11	73	theme	present	2175:2181	arg1	agents					2188:2193	The present RAFT agents	2171:2193	The present RAFT agents	2171:2193	The present RAFT agents were shown to eventually provide the phase separated structural polymeric materials in which 5.4 nm bioresource-spherical domains were periodically arrayed at the interval of about 10 nm.
25329608	9	74	theme	phase	1964:1968	arg1	morphology					1970:1979	a body centered cubic (BCC) phase morphology	1936:1979	a body centered cubic (BCC) phase morphology	1936:1979	Thus, these Mal5-hybrid amphiphilic BCP samples have a body centered cubic (BCC) phase morphology.
25329608	6	75	from	structures	1475:1484	arg1	states					1500:1505	their bulk states	1489:1505	their bulk states	1489:1505	The small-angle X-ray scattering (SAXS) measurements were accomplished for 2 and 3 to ensure their abilities to form phase separated structures in their bulk states with the increasing temperatures from 30 to 190 °C.
25329608	2	76	theme	azidopentyl	619:629	arg1	Mal5-N3					638:644	Mal5-N3	638:644	Mal5-N3	638:644	The synthesis was started from the catalyst-free direct N-glycosyl reaction of 5-azidopentylamine onto maltopentaose (Mal5) in dry methanol at room temperature and subsequent N-protected reaction with acetic anhydride, producing a stable oligosaccharide-building block, such as Mal5 with an azidopentyl group (Mal5-N3).
25329608	2	76	theme	azidopentyl	619:629	arg1	group					631:635	an azidopentyl group	616:635	an azidopentyl group (Mal5-N3)	616:645	The synthesis was started from the catalyst-free direct N-glycosyl reaction of 5-azidopentylamine onto maltopentaose (Mal5) in dry methanol at room temperature and subsequent N-protected reaction with acetic anhydride, producing a stable oligosaccharide-building block, such as Mal5 with an azidopentyl group (Mal5-N3).
25329608	6	77	theme	separated	1465:1473	arg1	structures					1475:1484	phase separated structures	1459:1484	phase separated structures in their bulk states	1459:1505	The small-angle X-ray scattering (SAXS) measurements were accomplished for 2 and 3 to ensure their abilities to form phase separated structures in their bulk states with the increasing temperatures from 30 to 190 °C.
25329608	1	78	theme	family	147:152	arg1	synthesis					128:136	the synthesis	124:136	the synthesis of a new family of oligosaccharide-conjugated functional molecules, which act as chain transfer agents (CTAs) for the reversible addition-fragmentation chain transfer (RAFT) polymerization	124:325	We now describe the synthesis of a new family of oligosaccharide-conjugated functional molecules, which act as chain transfer agents (CTAs) for the reversible addition-fragmentation chain transfer (RAFT) polymerization.
25329608	1	79	theme	oligosaccharide-conjugated	157:182	arg1	agents					234:239	chain transfer agents	219:239	chain transfer agents (CTAs) for the reversible addition-fragmentation chain transfer (RAFT) polymerization	219:325	We now describe the synthesis of a new family of oligosaccharide-conjugated functional molecules, which act as chain transfer agents (CTAs) for the reversible addition-fragmentation chain transfer (RAFT) polymerization.
25329608	1	79	theme	oligosaccharide-conjugated	157:182	arg1	molecules					195:203	oligosaccharide-conjugated functional molecules	157:203	oligosaccharide-conjugated functional molecules	157:203	We now describe the synthesis of a new family of oligosaccharide-conjugated functional molecules, which act as chain transfer agents (CTAs) for the reversible addition-fragmentation chain transfer (RAFT) polymerization.
25329608	6	80	from	°C	1555:1556	arg1	temperatures					1527:1538	the increasing temperatures	1512:1538	the increasing temperatures from 30 to 190 °C	1512:1556	The small-angle X-ray scattering (SAXS) measurements were accomplished for 2 and 3 to ensure their abilities to form phase separated structures in their bulk states with the increasing temperatures from 30 to 190 °C.
25329608	4	81	theme	RAFT	939:942	arg1	polymerizations					944:958	the RAFT polymerizations	935:958	the RAFT polymerizations of styrene (St) and methyl methacrylate (MMA)	935:1004	These reactions produced Mal5-modified macro-CTAs (Mal5-CTAs, 1), which were used for the RAFT polymerizations of styrene (St) and methyl methacrylate (MMA) in DMF.
25329608	8	82	theme	scattering	1776:1785	arg1	peaks					1787:1791	the primary scattering peaks	1764:1791	the primary scattering peaks q*	1764:1794	For both BCP samples, the primary scattering peaks q* were clearly observed together with the higher-ordered scattering peaks √2q* and √3q*.
25329608	2	83	with	reaction	515:522	arg1	anhydride					536:544	acetic anhydride	529:544	acetic anhydride	529:544	The synthesis was started from the catalyst-free direct N-glycosyl reaction of 5-azidopentylamine onto maltopentaose (Mal5) in dry methanol at room temperature and subsequent N-protected reaction with acetic anhydride, producing a stable oligosaccharide-building block, such as Mal5 with an azidopentyl group (Mal5-N3).
25329608	1	84	theme	molecules	195:203	arg1	family					147:152	a new family	141:152	a new family of oligosaccharide-conjugated functional molecules, which act as chain transfer agents (CTAs) for the reversible addition-fragmentation chain transfer (RAFT) polymerization	141:325	We now describe the synthesis of a new family of oligosaccharide-conjugated functional molecules, which act as chain transfer agents (CTAs) for the reversible addition-fragmentation chain transfer (RAFT) polymerization.
25329608	2	85	theme	acetic	529:534	arg1	anhydride					536:544	acetic anhydride	529:544	acetic anhydride	529:544	The synthesis was started from the catalyst-free direct N-glycosyl reaction of 5-azidopentylamine onto maltopentaose (Mal5) in dry methanol at room temperature and subsequent N-protected reaction with acetic anhydride, producing a stable oligosaccharide-building block, such as Mal5 with an azidopentyl group (Mal5-N3).
25329608	11	86	theme	nm	2379:2380	arg1	interval					2358:2365	the interval	2354:2365	the interval of about 10 nm	2354:2380	The present RAFT agents were shown to eventually provide the phase separated structural polymeric materials in which 5.4 nm bioresource-spherical domains were periodically arrayed at the interval of about 10 nm.
25329608	8	87	theme	q	1793:1793	arg1	*					1794:1794	q*	1793:1794	the primary scattering peaks q*	1764:1794	For both BCP samples, the primary scattering peaks q* were clearly observed together with the higher-ordered scattering peaks √2q* and √3q*.
25329608	2	88	theme	oligosaccharide-building	566:589	arg1	block					591:595	a stable oligosaccharide-building block	557:595	a stable oligosaccharide-building block	557:595	The synthesis was started from the catalyst-free direct N-glycosyl reaction of 5-azidopentylamine onto maltopentaose (Mal5) in dry methanol at room temperature and subsequent N-protected reaction with acetic anhydride, producing a stable oligosaccharide-building block, such as Mal5 with an azidopentyl group (Mal5-N3).
25329608	4	89	dep	macro-CTAs	888:897	arg1	Mal5-CTAs					900:908	Mal5-CTAs	900:908	Mal5-CTAs	900:908	These reactions produced Mal5-modified macro-CTAs (Mal5-CTAs, 1), which were used for the RAFT polymerizations of styrene (St) and methyl methacrylate (MMA) in DMF.
25329608	10	90	theme	domain	1986:1991	arg1	10.4					2081:2084	10.4	2081:2084	10.4	2081:2084	The domain spacing (d) values of the BCC morphology for 2 (ϕMal5 = 0.14) and 3 (ϕMal5 = 0.16) were 10.4 and 9.55 nm, respectively, which were determined using Bragg's relation (d = 2π/q*).
25329608	10	90	theme	domain	1986:1991	arg1	values					2005:2010	The domain spacing (d) values	1982:2010	The domain spacing (d) values of the BCC morphology for 2 (ϕMal5 = 0.14) and 3 (ϕMal5 = 0.16)	1982:2074	The domain spacing (d) values of the BCC morphology for 2 (ϕMal5 = 0.14) and 3 (ϕMal5 = 0.16) were 10.4 and 9.55 nm, respectively, which were determined using Bragg's relation (d = 2π/q*).
25329608	3	91	theme	ester	833:837	arg1	moieties					839:846	activated ester moieties	823:846	activated ester moieties	823:846	The azido group was hydrogenated using platinum dioxide (PtO2) as a catalyst to give Mal5 with aminopentyl group (Mal5-NH2), which was then reacted with CTA molecules bearing activated ester moieties.
25329608	6	92	theme	increasing	1516:1525	arg1	temperatures					1527:1538	the increasing temperatures	1512:1538	the increasing temperatures from 30 to 190 °C	1512:1556	The small-angle X-ray scattering (SAXS) measurements were accomplished for 2 and 3 to ensure their abilities to form phase separated structures in their bulk states with the increasing temperatures from 30 to 190 °C.
25329608	5	93	dep	600	1095:1097	arg1	to					1092:1093	to	1092:1093	to	1092:1093	The polymerizations were performed using the [M]0/[1]0 values ranging from 50 to 600, affording the Mal5-hybrid amphiphilic block copolymers (BCPs), such as Mal5-polystyrene (2) and Mal5-poly(methyl methacrylate) (3), with a quantitative end-functionality and the controlled molecular weights between 4310 and 20 300 g mol(-1).
25329608	5	94	theme	quantitative	1239:1250	arg1	end-functionality					1252:1268	a quantitative end-functionality	1237:1268	a quantitative end-functionality	1237:1268	The polymerizations were performed using the [M]0/[1]0 values ranging from 50 to 600, affording the Mal5-hybrid amphiphilic block copolymers (BCPs), such as Mal5-polystyrene (2) and Mal5-poly(methyl methacrylate) (3), with a quantitative end-functionality and the controlled molecular weights between 4310 and 20 300 g mol(-1).
25329608	4	95	theme	styrene	963:969	arg1	polymerizations					944:958	the RAFT polymerizations	935:958	the RAFT polymerizations of styrene (St) and methyl methacrylate (MMA)	935:1004	These reactions produced Mal5-modified macro-CTAs (Mal5-CTAs, 1), which were used for the RAFT polymerizations of styrene (St) and methyl methacrylate (MMA) in DMF.
25329608	7	96	theme	featured	1563:1570	arg1	results					1572:1578	The featured results	1559:1578	The featured results	1559:1578	The featured results were observed for 2 (ϕMal5 = 0.14) and 3 (ϕMal5 = 0.16) at temperatures above 100 °C, where ϕMal5 denotes the volume fraction of the Mal5 unit in the BCP sample.
25329608	8	97	theme	√3q	1877:1879	arg1	*					1880:1880	√3q*	1877:1880	√3q*	1877:1880	For both BCP samples, the primary scattering peaks q* were clearly observed together with the higher-ordered scattering peaks √2q* and √3q*.
25329608	11	98	theme	bioresource-spherical	2295:2315	arg1	domains					2317:2323	5.4 nm bioresource-spherical domains	2288:2323	5.4 nm bioresource-spherical domains	2288:2323	The present RAFT agents were shown to eventually provide the phase separated structural polymeric materials in which 5.4 nm bioresource-spherical domains were periodically arrayed at the interval of about 10 nm.
25329608	0	99	theme	RAFT	33:36	arg1	polymerization					38:51	RAFT polymerization	33:51	RAFT polymerization	33:51	Maltopentaose-conjugated CTA for RAFT polymerization generating nanostructured bioresource-block copolymer.
25329608	5	100	theme	amphiphilic	1126:1136	arg1	Mal5-poly					1196:1204	Mal5-poly	1196:1204	Mal5-poly(methyl methacrylate) (3)	1196:1229	The polymerizations were performed using the [M]0/[1]0 values ranging from 50 to 600, affording the Mal5-hybrid amphiphilic block copolymers (BCPs), such as Mal5-polystyrene (2) and Mal5-poly(methyl methacrylate) (3), with a quantitative end-functionality and the controlled molecular weights between 4310 and 20 300 g mol(-1).
25329608	5	100	theme	amphiphilic	1126:1136	arg1	Mal5-polystyrene					1171:1186	Mal5-polystyrene (2)	1171:1190	Mal5-polystyrene (2)	1171:1190	The polymerizations were performed using the [M]0/[1]0 values ranging from 50 to 600, affording the Mal5-hybrid amphiphilic block copolymers (BCPs), such as Mal5-polystyrene (2) and Mal5-poly(methyl methacrylate) (3), with a quantitative end-functionality and the controlled molecular weights between 4310 and 20 300 g mol(-1).
25329608	5	100	theme	amphiphilic	1126:1136	arg1	BCPs					1156:1159	BCPs	1156:1159	BCPs	1156:1159	The polymerizations were performed using the [M]0/[1]0 values ranging from 50 to 600, affording the Mal5-hybrid amphiphilic block copolymers (BCPs), such as Mal5-polystyrene (2) and Mal5-poly(methyl methacrylate) (3), with a quantitative end-functionality and the controlled molecular weights between 4310 and 20 300 g mol(-1).
25329608	5	100	theme	amphiphilic	1126:1136	arg1	copolymers					1144:1153	the Mal5-hybrid amphiphilic block copolymers	1110:1153	the Mal5-hybrid amphiphilic block copolymers (BCPs)	1110:1160	The polymerizations were performed using the [M]0/[1]0 values ranging from 50 to 600, affording the Mal5-hybrid amphiphilic block copolymers (BCPs), such as Mal5-polystyrene (2) and Mal5-poly(methyl methacrylate) (3), with a quantitative end-functionality and the controlled molecular weights between 4310 and 20 300 g mol(-1).
25329608	7	101	theme	unit	1718:1721	arg1	fraction					1697:1704	the volume fraction	1686:1704	the volume fraction of the Mal5 unit	1686:1721	The featured results were observed for 2 (ϕMal5 = 0.14) and 3 (ϕMal5 = 0.16) at temperatures above 100 °C, where ϕMal5 denotes the volume fraction of the Mal5 unit in the BCP sample.
25329608	6	102	theme	bulk	1495:1498	arg1	states					1500:1505	their bulk states	1489:1505	their bulk states	1489:1505	The small-angle X-ray scattering (SAXS) measurements were accomplished for 2 and 3 to ensure their abilities to form phase separated structures in their bulk states with the increasing temperatures from 30 to 190 °C.
25329608	11	103	theme	5.4	2288:2290	arg1	nm					2292:2293	nm	2292:2293	nm	2292:2293	The present RAFT agents were shown to eventually provide the phase separated structural polymeric materials in which 5.4 nm bioresource-spherical domains were periodically arrayed at the interval of about 10 nm.
25329608	3	104	theme	platinum	687:694	arg1	PtO2					705:708	PtO2	705:708	PtO2	705:708	The azido group was hydrogenated using platinum dioxide (PtO2) as a catalyst to give Mal5 with aminopentyl group (Mal5-NH2), which was then reacted with CTA molecules bearing activated ester moieties.
25329608	3	104	theme	platinum	687:694	arg1	dioxide					696:702	platinum dioxide	687:702	platinum dioxide (PtO2)	687:709	The azido group was hydrogenated using platinum dioxide (PtO2) as a catalyst to give Mal5 with aminopentyl group (Mal5-NH2), which was then reacted with CTA molecules bearing activated ester moieties.
25329608	3	104	theme	platinum	687:694	arg1	catalyst					716:723	a catalyst to give Mal5 with aminopentyl group (Mal5-NH2), which was then reacted with CTA molecules bearing activated ester moieties	714:846	a catalyst to give Mal5 with aminopentyl group (Mal5-NH2), which was then reacted with CTA molecules bearing activated ester moieties	714:846	The azido group was hydrogenated using platinum dioxide (PtO2) as a catalyst to give Mal5 with aminopentyl group (Mal5-NH2), which was then reacted with CTA molecules bearing activated ester moieties.
25329608	2	105	theme	dry	455:457	arg1	methanol					459:466	dry methanol	455:466	dry methanol at room temperature	455:486	The synthesis was started from the catalyst-free direct N-glycosyl reaction of 5-azidopentylamine onto maltopentaose (Mal5) in dry methanol at room temperature and subsequent N-protected reaction with acetic anhydride, producing a stable oligosaccharide-building block, such as Mal5 with an azidopentyl group (Mal5-N3).
25329608	8	106	theme	higher-ordered	1836:1849	arg1	peaks					1862:1866	the higher-ordered scattering peaks	1832:1866	the higher-ordered scattering peaks √2q* and √3q*	1832:1880	For both BCP samples, the primary scattering peaks q* were clearly observed together with the higher-ordered scattering peaks √2q* and √3q*.
25329608	4	107	theme	methyl	980:985	arg1	MMA					1001:1003	MMA	1001:1003	MMA	1001:1003	These reactions produced Mal5-modified macro-CTAs (Mal5-CTAs, 1), which were used for the RAFT polymerizations of styrene (St) and methyl methacrylate (MMA) in DMF.
25329608	4	107	theme	methyl	980:985	arg1	methacrylate					987:998	methyl methacrylate	980:998	methyl methacrylate (MMA)	980:1004	These reactions produced Mal5-modified macro-CTAs (Mal5-CTAs, 1), which were used for the RAFT polymerizations of styrene (St) and methyl methacrylate (MMA) in DMF.
25329608	0	108	theme	bioresource-block	79:95	arg1	copolymer					97:105	nanostructured bioresource-block copolymer	64:105	nanostructured bioresource-block copolymer	64:105	Maltopentaose-conjugated CTA for RAFT polymerization generating nanostructured bioresource-block copolymer.
25329608	2	109	theme	subsequent	492:501	arg1	reaction					515:522	subsequent N-protected reaction	492:522	subsequent N-protected reaction with acetic anhydride, producing a stable oligosaccharide-building block, such as Mal5 with an azidopentyl group (Mal5-N3)	492:645	The synthesis was started from the catalyst-free direct N-glycosyl reaction of 5-azidopentylamine onto maltopentaose (Mal5) in dry methanol at room temperature and subsequent N-protected reaction with acetic anhydride, producing a stable oligosaccharide-building block, such as Mal5 with an azidopentyl group (Mal5-N3).
25329608	5	110	dep	0/[1	1062:1065	arg1	values					1069:1074	0 values	1067:1074	0 values	1067:1074	The polymerizations were performed using the [M]0/[1]0 values ranging from 50 to 600, affording the Mal5-hybrid amphiphilic block copolymers (BCPs), such as Mal5-polystyrene (2) and Mal5-poly(methyl methacrylate) (3), with a quantitative end-functionality and the controlled molecular weights between 4310 and 20 300 g mol(-1).
25329608	2	111	theme	catalyst-free	363:375	arg1	reaction					395:402	the catalyst-free direct N-glycosyl reaction	359:402	the catalyst-free direct N-glycosyl reaction of 5-azidopentylamine onto maltopentaose (Mal5) in dry methanol at room temperature	359:486	The synthesis was started from the catalyst-free direct N-glycosyl reaction of 5-azidopentylamine onto maltopentaose (Mal5) in dry methanol at room temperature and subsequent N-protected reaction with acetic anhydride, producing a stable oligosaccharide-building block, such as Mal5 with an azidopentyl group (Mal5-N3).
25329608	3	112	with	Mal5	733:736	arg1	Mal5-NH2					762:769	Mal5-NH2	762:769	Mal5-NH2	762:769	The azido group was hydrogenated using platinum dioxide (PtO2) as a catalyst to give Mal5 with aminopentyl group (Mal5-NH2), which was then reacted with CTA molecules bearing activated ester moieties.
25329608	3	112	with	Mal5	733:736	arg1	group					755:759	aminopentyl group	743:759	aminopentyl group (Mal5-NH2)	743:770	The azido group was hydrogenated using platinum dioxide (PtO2) as a catalyst to give Mal5 with aminopentyl group (Mal5-NH2), which was then reacted with CTA molecules bearing activated ester moieties.
25329608	11	113	theme	separated	2238:2246	arg1	materials					2269:2277	the phase separated structural polymeric materials	2228:2277	the phase separated structural polymeric materials in which 5.4 nm bioresource-spherical domains were periodically arrayed at the interval of about 10 nm	2228:2380	The present RAFT agents were shown to eventually provide the phase separated structural polymeric materials in which 5.4 nm bioresource-spherical domains were periodically arrayed at the interval of about 10 nm.
25329608	2	114	with	Mal5	606:609	arg1	Mal5-N3					638:644	Mal5-N3	638:644	Mal5-N3	638:644	The synthesis was started from the catalyst-free direct N-glycosyl reaction of 5-azidopentylamine onto maltopentaose (Mal5) in dry methanol at room temperature and subsequent N-protected reaction with acetic anhydride, producing a stable oligosaccharide-building block, such as Mal5 with an azidopentyl group (Mal5-N3).
25329608	2	114	with	Mal5	606:609	arg1	group					631:635	an azidopentyl group	616:635	an azidopentyl group (Mal5-N3)	616:645	The synthesis was started from the catalyst-free direct N-glycosyl reaction of 5-azidopentylamine onto maltopentaose (Mal5) in dry methanol at room temperature and subsequent N-protected reaction with acetic anhydride, producing a stable oligosaccharide-building block, such as Mal5 with an azidopentyl group (Mal5-N3).
25329608	5	115	theme	Mal5-hybrid	1114:1124	arg1	Mal5-poly					1196:1204	Mal5-poly	1196:1204	Mal5-poly(methyl methacrylate) (3)	1196:1229	The polymerizations were performed using the [M]0/[1]0 values ranging from 50 to 600, affording the Mal5-hybrid amphiphilic block copolymers (BCPs), such as Mal5-polystyrene (2) and Mal5-poly(methyl methacrylate) (3), with a quantitative end-functionality and the controlled molecular weights between 4310 and 20 300 g mol(-1).
25329608	5	115	theme	Mal5-hybrid	1114:1124	arg1	Mal5-polystyrene					1171:1186	Mal5-polystyrene (2)	1171:1190	Mal5-polystyrene (2)	1171:1190	The polymerizations were performed using the [M]0/[1]0 values ranging from 50 to 600, affording the Mal5-hybrid amphiphilic block copolymers (BCPs), such as Mal5-polystyrene (2) and Mal5-poly(methyl methacrylate) (3), with a quantitative end-functionality and the controlled molecular weights between 4310 and 20 300 g mol(-1).
25329608	5	115	theme	Mal5-hybrid	1114:1124	arg1	BCPs					1156:1159	BCPs	1156:1159	BCPs	1156:1159	The polymerizations were performed using the [M]0/[1]0 values ranging from 50 to 600, affording the Mal5-hybrid amphiphilic block copolymers (BCPs), such as Mal5-polystyrene (2) and Mal5-poly(methyl methacrylate) (3), with a quantitative end-functionality and the controlled molecular weights between 4310 and 20 300 g mol(-1).
25329608	5	115	theme	Mal5-hybrid	1114:1124	arg1	copolymers					1144:1153	the Mal5-hybrid amphiphilic block copolymers	1110:1153	the Mal5-hybrid amphiphilic block copolymers (BCPs)	1110:1160	The polymerizations were performed using the [M]0/[1]0 values ranging from 50 to 600, affording the Mal5-hybrid amphiphilic block copolymers (BCPs), such as Mal5-polystyrene (2) and Mal5-poly(methyl methacrylate) (3), with a quantitative end-functionality and the controlled molecular weights between 4310 and 20 300 g mol(-1).
25329608	1	116	theme	reversible	256:265	arg1	polymerization					312:325	the reversible addition-fragmentation chain transfer (RAFT) polymerization	252:325	the reversible addition-fragmentation chain transfer (RAFT) polymerization	252:325	We now describe the synthesis of a new family of oligosaccharide-conjugated functional molecules, which act as chain transfer agents (CTAs) for the reversible addition-fragmentation chain transfer (RAFT) polymerization.
25329608	5	117	theme	controlled	1278:1287	arg1	Mal5-poly					1196:1204	Mal5-poly	1196:1204	Mal5-poly(methyl methacrylate) (3)	1196:1229	The polymerizations were performed using the [M]0/[1]0 values ranging from 50 to 600, affording the Mal5-hybrid amphiphilic block copolymers (BCPs), such as Mal5-polystyrene (2) and Mal5-poly(methyl methacrylate) (3), with a quantitative end-functionality and the controlled molecular weights between 4310 and 20 300 g mol(-1).
25329608	5	117	theme	controlled	1278:1287	arg1	Mal5-polystyrene					1171:1186	Mal5-polystyrene (2)	1171:1190	Mal5-polystyrene (2)	1171:1190	The polymerizations were performed using the [M]0/[1]0 values ranging from 50 to 600, affording the Mal5-hybrid amphiphilic block copolymers (BCPs), such as Mal5-polystyrene (2) and Mal5-poly(methyl methacrylate) (3), with a quantitative end-functionality and the controlled molecular weights between 4310 and 20 300 g mol(-1).
25329608	5	117	theme	controlled	1278:1287	arg1	weights					1299:1305	the controlled molecular weights	1274:1305	the controlled molecular weights between 4310 and 20 300 g mol(-1)	1274:1339	The polymerizations were performed using the [M]0/[1]0 values ranging from 50 to 600, affording the Mal5-hybrid amphiphilic block copolymers (BCPs), such as Mal5-polystyrene (2) and Mal5-poly(methyl methacrylate) (3), with a quantitative end-functionality and the controlled molecular weights between 4310 and 20 300 g mol(-1).
25329608	4	118	used	used	926:929	arg2	macro-CTAs					888:897	Mal5-modified macro-CTAs	874:897	Mal5-modified macro-CTAs (Mal5-CTAs, 1)	874:912	These reactions produced Mal5-modified macro-CTAs (Mal5-CTAs, 1), which were used for the RAFT polymerizations of styrene (St) and methyl methacrylate (MMA) in DMF.
26212959	2	0	dep	X3	511:512	arg1	U/mg					521:524	15-75 U/mg	515:524	X3: 15-75 U/mg	511:524	A central composite design (CCD) was employed for experimental design and analysis of the results to obtain the best possible combination of NaOH concentration (X1: 0.4-1.2 mol/l), alkali treatment time (X2: 6-30 h), enzyme concentration (X3: 15-75 U/mg) and hydrolysis time (X4: 12-60 h) for maximum PSC extraction.
26212959	3	1	theme	experimental	593:604	arg1	data					606:609	The experimental data	589:609	The experimental data obtained	589:618	The experimental data obtained were fitted to a second-order polynomial equation using multiple regression analysis and analyzed by appropriate statistical methods.
26212959	4	2	theme	optimum	780:786	arg1	conditions					799:808	optimum extraction conditions	780:808	optimum extraction conditions	780:808	According to the results, optimum extraction conditions were as follows: NaOH concentration of 0.76 mol/l, alkali treatment time of 18 h, enzyme concentration of 50 U/mg and hydrolysis time of 43.42 h.
26212959	2	3	dep	X4	548:549	arg1	h					558:558	12-60 h	552:558	X4: 12-60 h	548:558	A central composite design (CCD) was employed for experimental design and analysis of the results to obtain the best possible combination of NaOH concentration (X1: 0.4-1.2 mol/l), alkali treatment time (X2: 6-30 h), enzyme concentration (X3: 15-75 U/mg) and hydrolysis time (X4: 12-60 h) for maximum PSC extraction.
26212959	0	4	theme	surface	86:92	arg1	RSM					107:109	RSM	107:109	RSM	107:109	Extraction optimization of pepsin-soluble collagen from eggshell membrane by response surface methodology (RSM).
26212959	0	4	theme	surface	86:92	arg1	methodology					94:104	response surface methodology	77:104	response surface methodology (RSM)	77:110	Extraction optimization of pepsin-soluble collagen from eggshell membrane by response surface methodology (RSM).
26212959	0	5	from	optimization	11:22	arg1	membrane					65:72	eggshell membrane	56:72	eggshell membrane	56:72	Extraction optimization of pepsin-soluble collagen from eggshell membrane by response surface methodology (RSM).
26212959	4	6	theme	h	953:953	arg1	concentration					899:911	enzyme concentration	892:911	enzyme concentration of 50 U/mg	892:922	According to the results, optimum extraction conditions were as follows: NaOH concentration of 0.76 mol/l, alkali treatment time of 18 h, enzyme concentration of 50 U/mg and hydrolysis time of 43.42 h.
26212959	4	6	theme	h	953:953	arg1	concentration					832:844	NaOH concentration	827:844	NaOH concentration of 0.76 mol/l	827:858	According to the results, optimum extraction conditions were as follows: NaOH concentration of 0.76 mol/l, alkali treatment time of 18 h, enzyme concentration of 50 U/mg and hydrolysis time of 43.42 h.
26212959	4	6	theme	h	953:953	arg1	time					939:942	hydrolysis time	928:942	hydrolysis time of 43.42 h	928:953	According to the results, optimum extraction conditions were as follows: NaOH concentration of 0.76 mol/l, alkali treatment time of 18 h, enzyme concentration of 50 U/mg and hydrolysis time of 43.42 h.
26212959	4	6	theme	h	953:953	arg1	time					878:881	alkali treatment time	861:881	alkali treatment time of 18 h	861:889	According to the results, optimum extraction conditions were as follows: NaOH concentration of 0.76 mol/l, alkali treatment time of 18 h, enzyme concentration of 50 U/mg and hydrolysis time of 43.42 h.
26212959	4	7	theme	NaOH	827:830	arg1	concentration					832:844	NaOH concentration	827:844	NaOH concentration of 0.76 mol/l	827:858	According to the results, optimum extraction conditions were as follows: NaOH concentration of 0.76 mol/l, alkali treatment time of 18 h, enzyme concentration of 50 U/mg and hydrolysis time of 43.42 h.
26212959	2	8	theme	maximum	565:571	arg1	extraction					577:586	maximum PSC extraction	565:586	maximum PSC extraction	565:586	A central composite design (CCD) was employed for experimental design and analysis of the results to obtain the best possible combination of NaOH concentration (X1: 0.4-1.2 mol/l), alkali treatment time (X2: 6-30 h), enzyme concentration (X3: 15-75 U/mg) and hydrolysis time (X4: 12-60 h) for maximum PSC extraction.
26212959	1	9	used	used	152:155	arg2	RSM					143:145	RSM	143:145	RSM	143:145	Response surface methodology (RSM) was used to investigate the effect of extraction-process variables on pepsin-soluble collagen (PSC) from eggshell membrane.
26212959	1	9	used	used	152:155	arg2	methodology					130:140	Response surface methodology	113:140	Response surface methodology (RSM)	113:146	Response surface methodology (RSM) was used to investigate the effect of extraction-process variables on pepsin-soluble collagen (PSC) from eggshell membrane.
26212959	1	10	theme	eggshell	253:260	arg1	membrane					262:269	eggshell membrane	253:269	eggshell membrane	253:269	Response surface methodology (RSM) was used to investigate the effect of extraction-process variables on pepsin-soluble collagen (PSC) from eggshell membrane.
26212959	4	11	theme	mol/l	854:858	arg1	concentration					899:911	enzyme concentration	892:911	enzyme concentration of 50 U/mg	892:922	According to the results, optimum extraction conditions were as follows: NaOH concentration of 0.76 mol/l, alkali treatment time of 18 h, enzyme concentration of 50 U/mg and hydrolysis time of 43.42 h.
26212959	4	11	theme	mol/l	854:858	arg1	concentration					832:844	NaOH concentration	827:844	NaOH concentration of 0.76 mol/l	827:858	According to the results, optimum extraction conditions were as follows: NaOH concentration of 0.76 mol/l, alkali treatment time of 18 h, enzyme concentration of 50 U/mg and hydrolysis time of 43.42 h.
26212959	4	11	theme	mol/l	854:858	arg1	time					939:942	hydrolysis time	928:942	hydrolysis time of 43.42 h	928:953	According to the results, optimum extraction conditions were as follows: NaOH concentration of 0.76 mol/l, alkali treatment time of 18 h, enzyme concentration of 50 U/mg and hydrolysis time of 43.42 h.
26212959	4	11	theme	mol/l	854:858	arg1	time					878:881	alkali treatment time	861:881	alkali treatment time of 18 h	861:889	According to the results, optimum extraction conditions were as follows: NaOH concentration of 0.76 mol/l, alkali treatment time of 18 h, enzyme concentration of 50 U/mg and hydrolysis time of 43.42 h.
26212959	0	12	from	membrane	65:72	arg1	optimization					11:22	Extraction optimization	0:22	Extraction optimization of pepsin-soluble collagen from eggshell membrane by response surface methodology (RSM).	0:111	Extraction optimization of pepsin-soluble collagen from eggshell membrane by response surface methodology (RSM).
26212959	0	12	from	membrane	65:72	arg1	collagen					42:49	pepsin-soluble collagen	27:49	pepsin-soluble collagen from eggshell membrane	27:72	Extraction optimization of pepsin-soluble collagen from eggshell membrane by response surface methodology (RSM).
26212959	4	13	theme	extraction	788:797	arg1	conditions					799:808	optimum extraction conditions	780:808	optimum extraction conditions	780:808	According to the results, optimum extraction conditions were as follows: NaOH concentration of 0.76 mol/l, alkali treatment time of 18 h, enzyme concentration of 50 U/mg and hydrolysis time of 43.42 h.
26212959	2	14	theme	central	274:280	arg1	CCD					300:302	CCD	300:302	CCD	300:302	A central composite design (CCD) was employed for experimental design and analysis of the results to obtain the best possible combination of NaOH concentration (X1: 0.4-1.2 mol/l), alkali treatment time (X2: 6-30 h), enzyme concentration (X3: 15-75 U/mg) and hydrolysis time (X4: 12-60 h) for maximum PSC extraction.
26212959	2	14	theme	central	274:280	arg1	design					292:297	A central composite design	272:297	A central composite design (CCD)	272:303	A central composite design (CCD) was employed for experimental design and analysis of the results to obtain the best possible combination of NaOH concentration (X1: 0.4-1.2 mol/l), alkali treatment time (X2: 6-30 h), enzyme concentration (X3: 15-75 U/mg) and hydrolysis time (X4: 12-60 h) for maximum PSC extraction.
26212959	3	15	theme	statistical	733:743	arg1	methods					745:751	appropriate statistical methods	721:751	appropriate statistical methods	721:751	The experimental data obtained were fitted to a second-order polynomial equation using multiple regression analysis and analyzed by appropriate statistical methods.
26212959	4	16	theme	treatment	868:876	arg1	time					878:881	alkali treatment time	861:881	alkali treatment time of 18 h	861:889	According to the results, optimum extraction conditions were as follows: NaOH concentration of 0.76 mol/l, alkali treatment time of 18 h, enzyme concentration of 50 U/mg and hydrolysis time of 43.42 h.
26212959	2	17	theme	experimental	322:333	arg1	design					335:340	experimental design	322:340	experimental design	322:340	A central composite design (CCD) was employed for experimental design and analysis of the results to obtain the best possible combination of NaOH concentration (X1: 0.4-1.2 mol/l), alkali treatment time (X2: 6-30 h), enzyme concentration (X3: 15-75 U/mg) and hydrolysis time (X4: 12-60 h) for maximum PSC extraction.
26212959	2	18	theme	possible	389:396	arg1	combination					398:408	the best possible combination	380:408	the best possible combination of NaOH concentration (X1: 0.4-1.2 mol/l), alkali treatment time (X2: 6-30 h), enzyme concentration (X3: 15-75 U/mg) and hydrolysis time (X4: 12-60 h)	380:559	A central composite design (CCD) was employed for experimental design and analysis of the results to obtain the best possible combination of NaOH concentration (X1: 0.4-1.2 mol/l), alkali treatment time (X2: 6-30 h), enzyme concentration (X3: 15-75 U/mg) and hydrolysis time (X4: 12-60 h) for maximum PSC extraction.
26212959	3	19	theme	appropriate	721:731	arg1	methods					745:751	appropriate statistical methods	721:751	appropriate statistical methods	721:751	The experimental data obtained were fitted to a second-order polynomial equation using multiple regression analysis and analyzed by appropriate statistical methods.
26212959	0	20	theme	eggshell	56:63	arg1	membrane					65:72	eggshell membrane	56:72	eggshell membrane	56:72	Extraction optimization of pepsin-soluble collagen from eggshell membrane by response surface methodology (RSM).
26212959	5	21	theme	optimal	996:1002	arg1	conditions					1004:1013	optimal conditions	996:1013	optimal conditions	996:1013	The experimental extraction yield under optimal conditions was found to be 30.049%, which is in good agreement with the predicted value of 30.054%.
26212959	2	22	theme	time	542:545	arg1	combination					398:408	the best possible combination	380:408	the best possible combination of NaOH concentration (X1: 0.4-1.2 mol/l), alkali treatment time (X2: 6-30 h), enzyme concentration (X3: 15-75 U/mg) and hydrolysis time (X4: 12-60 h)	380:559	A central composite design (CCD) was employed for experimental design and analysis of the results to obtain the best possible combination of NaOH concentration (X1: 0.4-1.2 mol/l), alkali treatment time (X2: 6-30 h), enzyme concentration (X3: 15-75 U/mg) and hydrolysis time (X4: 12-60 h) for maximum PSC extraction.
26212959	2	23	theme	PSC	573:575	arg1	extraction					577:586	maximum PSC extraction	565:586	maximum PSC extraction	565:586	A central composite design (CCD) was employed for experimental design and analysis of the results to obtain the best possible combination of NaOH concentration (X1: 0.4-1.2 mol/l), alkali treatment time (X2: 6-30 h), enzyme concentration (X3: 15-75 U/mg) and hydrolysis time (X4: 12-60 h) for maximum PSC extraction.
26212959	2	24	theme	concentration	418:430	arg1	combination					398:408	the best possible combination	380:408	the best possible combination of NaOH concentration (X1: 0.4-1.2 mol/l), alkali treatment time (X2: 6-30 h), enzyme concentration (X3: 15-75 U/mg) and hydrolysis time (X4: 12-60 h)	380:559	A central composite design (CCD) was employed for experimental design and analysis of the results to obtain the best possible combination of NaOH concentration (X1: 0.4-1.2 mol/l), alkali treatment time (X2: 6-30 h), enzyme concentration (X3: 15-75 U/mg) and hydrolysis time (X4: 12-60 h) for maximum PSC extraction.
26212959	4	25	theme	U/mg	919:922	arg1	concentration					899:911	enzyme concentration	892:911	enzyme concentration of 50 U/mg	892:922	According to the results, optimum extraction conditions were as follows: NaOH concentration of 0.76 mol/l, alkali treatment time of 18 h, enzyme concentration of 50 U/mg and hydrolysis time of 43.42 h.
26212959	4	25	theme	U/mg	919:922	arg1	concentration					832:844	NaOH concentration	827:844	NaOH concentration of 0.76 mol/l	827:858	According to the results, optimum extraction conditions were as follows: NaOH concentration of 0.76 mol/l, alkali treatment time of 18 h, enzyme concentration of 50 U/mg and hydrolysis time of 43.42 h.
26212959	4	25	theme	U/mg	919:922	arg1	time					939:942	hydrolysis time	928:942	hydrolysis time of 43.42 h	928:953	According to the results, optimum extraction conditions were as follows: NaOH concentration of 0.76 mol/l, alkali treatment time of 18 h, enzyme concentration of 50 U/mg and hydrolysis time of 43.42 h.
26212959	4	25	theme	U/mg	919:922	arg1	time					878:881	alkali treatment time	861:881	alkali treatment time of 18 h	861:889	According to the results, optimum extraction conditions were as follows: NaOH concentration of 0.76 mol/l, alkali treatment time of 18 h, enzyme concentration of 50 U/mg and hydrolysis time of 43.42 h.
26212959	4	26	dep	were	810:813	arg1	concentration					899:911	enzyme concentration	892:911	enzyme concentration of 50 U/mg	892:922	According to the results, optimum extraction conditions were as follows: NaOH concentration of 0.76 mol/l, alkali treatment time of 18 h, enzyme concentration of 50 U/mg and hydrolysis time of 43.42 h.
26212959	4	26	dep	were	810:813	arg1	concentration					832:844	NaOH concentration	827:844	NaOH concentration of 0.76 mol/l	827:858	According to the results, optimum extraction conditions were as follows: NaOH concentration of 0.76 mol/l, alkali treatment time of 18 h, enzyme concentration of 50 U/mg and hydrolysis time of 43.42 h.
26212959	4	26	dep	were	810:813	arg1	time					939:942	hydrolysis time	928:942	hydrolysis time of 43.42 h	928:953	According to the results, optimum extraction conditions were as follows: NaOH concentration of 0.76 mol/l, alkali treatment time of 18 h, enzyme concentration of 50 U/mg and hydrolysis time of 43.42 h.
26212959	4	26	dep	were	810:813	arg1	time					878:881	alkali treatment time	861:881	alkali treatment time of 18 h	861:889	According to the results, optimum extraction conditions were as follows: NaOH concentration of 0.76 mol/l, alkali treatment time of 18 h, enzyme concentration of 50 U/mg and hydrolysis time of 43.42 h.
26212959	2	27	theme	hydrolysis	531:540	arg1	time					542:545	hydrolysis time	531:545	hydrolysis time (X4: 12-60 h)	531:559	A central composite design (CCD) was employed for experimental design and analysis of the results to obtain the best possible combination of NaOH concentration (X1: 0.4-1.2 mol/l), alkali treatment time (X2: 6-30 h), enzyme concentration (X3: 15-75 U/mg) and hydrolysis time (X4: 12-60 h) for maximum PSC extraction.
26212959	2	28	theme	treatment	460:468	arg1	time					470:473	alkali treatment time	453:473	alkali treatment time (X2: 6-30 h)	453:486	A central composite design (CCD) was employed for experimental design and analysis of the results to obtain the best possible combination of NaOH concentration (X1: 0.4-1.2 mol/l), alkali treatment time (X2: 6-30 h), enzyme concentration (X3: 15-75 U/mg) and hydrolysis time (X4: 12-60 h) for maximum PSC extraction.
26212959	2	29	theme	results	362:368	arg1	analysis					346:353	analysis	346:353	analysis	346:353	A central composite design (CCD) was employed for experimental design and analysis of the results to obtain the best possible combination of NaOH concentration (X1: 0.4-1.2 mol/l), alkali treatment time (X2: 6-30 h), enzyme concentration (X3: 15-75 U/mg) and hydrolysis time (X4: 12-60 h) for maximum PSC extraction.
26212959	2	29	theme	results	362:368	arg1	design					335:340	experimental design	322:340	experimental design	322:340	A central composite design (CCD) was employed for experimental design and analysis of the results to obtain the best possible combination of NaOH concentration (X1: 0.4-1.2 mol/l), alkali treatment time (X2: 6-30 h), enzyme concentration (X3: 15-75 U/mg) and hydrolysis time (X4: 12-60 h) for maximum PSC extraction.
26212959	0	30	theme	Extraction	0:9	arg1	optimization					11:22	Extraction optimization	0:22	Extraction optimization of pepsin-soluble collagen from eggshell membrane by response surface methodology (RSM).	0:111	Extraction optimization of pepsin-soluble collagen from eggshell membrane by response surface methodology (RSM).
26212959	3	31	theme	second-order	637:648	arg1	equation					661:668	a second-order polynomial equation	635:668	a second-order polynomial equation using multiple regression analysis	635:703	The experimental data obtained were fitted to a second-order polynomial equation using multiple regression analysis and analyzed by appropriate statistical methods.
26212959	2	32	theme	NaOH	413:416	arg1	concentration					418:430	NaOH concentration	413:430	NaOH concentration (X1: 0.4-1.2 mol/l)	413:450	A central composite design (CCD) was employed for experimental design and analysis of the results to obtain the best possible combination of NaOH concentration (X1: 0.4-1.2 mol/l), alkali treatment time (X2: 6-30 h), enzyme concentration (X3: 15-75 U/mg) and hydrolysis time (X4: 12-60 h) for maximum PSC extraction.
26212959	2	32	theme	NaOH	413:416	arg1	mol/l					445:449	X1: 0.4-1.2 mol/l	433:449	X1: 0.4-1.2 mol/l	433:449	A central composite design (CCD) was employed for experimental design and analysis of the results to obtain the best possible combination of NaOH concentration (X1: 0.4-1.2 mol/l), alkali treatment time (X2: 6-30 h), enzyme concentration (X3: 15-75 U/mg) and hydrolysis time (X4: 12-60 h) for maximum PSC extraction.
26212959	3	33	theme	polynomial	650:659	arg1	equation					661:668	a second-order polynomial equation	635:668	a second-order polynomial equation using multiple regression analysis	635:703	The experimental data obtained were fitted to a second-order polynomial equation using multiple regression analysis and analyzed by appropriate statistical methods.
26212959	2	34	dep	time	470:473	arg1	X2					476:477	X2	476:477	X2: 6-30 h	476:485	A central composite design (CCD) was employed for experimental design and analysis of the results to obtain the best possible combination of NaOH concentration (X1: 0.4-1.2 mol/l), alkali treatment time (X2: 6-30 h), enzyme concentration (X3: 15-75 U/mg) and hydrolysis time (X4: 12-60 h) for maximum PSC extraction.
26212959	2	35	dep	time	542:545	arg1	X4					548:549	X4	548:549	X4: 12-60 h	548:558	A central composite design (CCD) was employed for experimental design and analysis of the results to obtain the best possible combination of NaOH concentration (X1: 0.4-1.2 mol/l), alkali treatment time (X2: 6-30 h), enzyme concentration (X3: 15-75 U/mg) and hydrolysis time (X4: 12-60 h) for maximum PSC extraction.
26212959	5	36	theme	good	1052:1055	arg1	agreement					1057:1065	good agreement	1052:1065	good agreement with the predicted value of 30.054%	1052:1101	The experimental extraction yield under optimal conditions was found to be 30.049%, which is in good agreement with the predicted value of 30.054%.
26212959	1	37	from	membrane	262:269	arg1	PSC					243:245	PSC	243:245	PSC	243:245	Response surface methodology (RSM) was used to investigate the effect of extraction-process variables on pepsin-soluble collagen (PSC) from eggshell membrane.
26212959	1	37	from	membrane	262:269	arg1	collagen					233:240	pepsin-soluble collagen	218:240	pepsin-soluble collagen (PSC) from eggshell membrane	218:269	Response surface methodology (RSM) was used to investigate the effect of extraction-process variables on pepsin-soluble collagen (PSC) from eggshell membrane.
26212959	1	38	theme	extraction-process	186:203	arg1	variables					205:213	extraction-process variables	186:213	extraction-process variables	186:213	Response surface methodology (RSM) was used to investigate the effect of extraction-process variables on pepsin-soluble collagen (PSC) from eggshell membrane.
26212959	4	39	theme	enzyme	892:897	arg1	concentration					899:911	enzyme concentration	892:911	enzyme concentration of 50 U/mg	892:922	According to the results, optimum extraction conditions were as follows: NaOH concentration of 0.76 mol/l, alkali treatment time of 18 h, enzyme concentration of 50 U/mg and hydrolysis time of 43.42 h.
26212959	2	40	theme	best	384:387	arg1	combination					398:408	the best possible combination	380:408	the best possible combination of NaOH concentration (X1: 0.4-1.2 mol/l), alkali treatment time (X2: 6-30 h), enzyme concentration (X3: 15-75 U/mg) and hydrolysis time (X4: 12-60 h)	380:559	A central composite design (CCD) was employed for experimental design and analysis of the results to obtain the best possible combination of NaOH concentration (X1: 0.4-1.2 mol/l), alkali treatment time (X2: 6-30 h), enzyme concentration (X3: 15-75 U/mg) and hydrolysis time (X4: 12-60 h) for maximum PSC extraction.
26212959	4	41	theme	hydrolysis	928:937	arg1	time					939:942	hydrolysis time	928:942	hydrolysis time of 43.42 h	928:953	According to the results, optimum extraction conditions were as follows: NaOH concentration of 0.76 mol/l, alkali treatment time of 18 h, enzyme concentration of 50 U/mg and hydrolysis time of 43.42 h.
26212959	1	42	theme	variables	205:213	arg1	effect					176:181	the effect	172:181	the effect of extraction-process variables on pepsin-soluble collagen (PSC) from eggshell membrane	172:269	Response surface methodology (RSM) was used to investigate the effect of extraction-process variables on pepsin-soluble collagen (PSC) from eggshell membrane.
26212959	0	43	theme	collagen	42:49	arg1	optimization					11:22	Extraction optimization	0:22	Extraction optimization of pepsin-soluble collagen from eggshell membrane by response surface methodology (RSM).	0:111	Extraction optimization of pepsin-soluble collagen from eggshell membrane by response surface methodology (RSM).
26212959	3	44	theme	multiple	676:683	arg1	analysis					696:703	multiple regression analysis	676:703	multiple regression analysis	676:703	The experimental data obtained were fitted to a second-order polynomial equation using multiple regression analysis and analyzed by appropriate statistical methods.
26212959	2	45	dep	X2	476:477	arg1	h					485:485	6-30 h	480:485	X2: 6-30 h	476:485	A central composite design (CCD) was employed for experimental design and analysis of the results to obtain the best possible combination of NaOH concentration (X1: 0.4-1.2 mol/l), alkali treatment time (X2: 6-30 h), enzyme concentration (X3: 15-75 U/mg) and hydrolysis time (X4: 12-60 h) for maximum PSC extraction.
26212959	5	46	theme	experimental	960:971	arg1	yield					984:988	The experimental extraction yield	956:988	The experimental extraction yield under optimal conditions	956:1013	The experimental extraction yield under optimal conditions was found to be 30.049%, which is in good agreement with the predicted value of 30.054%.
26212959	5	46	theme	experimental	960:971	arg1	%					1037:1037	30.049%	1031:1037	30.049%	1031:1037	The experimental extraction yield under optimal conditions was found to be 30.049%, which is in good agreement with the predicted value of 30.054%.
26212959	0	47	theme	pepsin-soluble	27:40	arg1	collagen					42:49	pepsin-soluble collagen	27:49	pepsin-soluble collagen from eggshell membrane	27:72	Extraction optimization of pepsin-soluble collagen from eggshell membrane by response surface methodology (RSM).
26212959	2	48	theme	time	470:473	arg1	combination					398:408	the best possible combination	380:408	the best possible combination of NaOH concentration (X1: 0.4-1.2 mol/l), alkali treatment time (X2: 6-30 h), enzyme concentration (X3: 15-75 U/mg) and hydrolysis time (X4: 12-60 h)	380:559	A central composite design (CCD) was employed for experimental design and analysis of the results to obtain the best possible combination of NaOH concentration (X1: 0.4-1.2 mol/l), alkali treatment time (X2: 6-30 h), enzyme concentration (X3: 15-75 U/mg) and hydrolysis time (X4: 12-60 h) for maximum PSC extraction.
26212959	3	49	theme	regression	685:694	arg1	analysis					696:703	multiple regression analysis	676:703	multiple regression analysis	676:703	The experimental data obtained were fitted to a second-order polynomial equation using multiple regression analysis and analyzed by appropriate statistical methods.
26212959	5	50	theme	extraction	973:982	arg1	yield					984:988	The experimental extraction yield	956:988	The experimental extraction yield under optimal conditions	956:1013	The experimental extraction yield under optimal conditions was found to be 30.049%, which is in good agreement with the predicted value of 30.054%.
26212959	5	50	theme	extraction	973:982	arg1	%					1037:1037	30.049%	1031:1037	30.049%	1031:1037	The experimental extraction yield under optimal conditions was found to be 30.049%, which is in good agreement with the predicted value of 30.054%.
26212959	1	51	theme	Response	113:120	arg1	methodology					130:140	Response surface methodology	113:140	Response surface methodology (RSM)	113:146	Response surface methodology (RSM) was used to investigate the effect of extraction-process variables on pepsin-soluble collagen (PSC) from eggshell membrane.
26212959	1	51	theme	Response	113:120	arg1	RSM					143:145	RSM	143:145	RSM	143:145	Response surface methodology (RSM) was used to investigate the effect of extraction-process variables on pepsin-soluble collagen (PSC) from eggshell membrane.
26212959	2	52	theme	X1	433:434	arg1	concentration					418:430	NaOH concentration	413:430	NaOH concentration (X1: 0.4-1.2 mol/l)	413:450	A central composite design (CCD) was employed for experimental design and analysis of the results to obtain the best possible combination of NaOH concentration (X1: 0.4-1.2 mol/l), alkali treatment time (X2: 6-30 h), enzyme concentration (X3: 15-75 U/mg) and hydrolysis time (X4: 12-60 h) for maximum PSC extraction.
26212959	2	52	theme	X1	433:434	arg1	mol/l					445:449	X1: 0.4-1.2 mol/l	433:449	X1: 0.4-1.2 mol/l	433:449	A central composite design (CCD) was employed for experimental design and analysis of the results to obtain the best possible combination of NaOH concentration (X1: 0.4-1.2 mol/l), alkali treatment time (X2: 6-30 h), enzyme concentration (X3: 15-75 U/mg) and hydrolysis time (X4: 12-60 h) for maximum PSC extraction.
26212959	1	53	theme	surface	122:128	arg1	methodology					130:140	Response surface methodology	113:140	Response surface methodology (RSM)	113:146	Response surface methodology (RSM) was used to investigate the effect of extraction-process variables on pepsin-soluble collagen (PSC) from eggshell membrane.
26212959	1	53	theme	surface	122:128	arg1	RSM					143:145	RSM	143:145	RSM	143:145	Response surface methodology (RSM) was used to investigate the effect of extraction-process variables on pepsin-soluble collagen (PSC) from eggshell membrane.
26212959	2	54	theme	concentration	496:508	arg1	combination					398:408	the best possible combination	380:408	the best possible combination of NaOH concentration (X1: 0.4-1.2 mol/l), alkali treatment time (X2: 6-30 h), enzyme concentration (X3: 15-75 U/mg) and hydrolysis time (X4: 12-60 h)	380:559	A central composite design (CCD) was employed for experimental design and analysis of the results to obtain the best possible combination of NaOH concentration (X1: 0.4-1.2 mol/l), alkali treatment time (X2: 6-30 h), enzyme concentration (X3: 15-75 U/mg) and hydrolysis time (X4: 12-60 h) for maximum PSC extraction.
26212959	5	55	with	agreement	1057:1065	arg1	value					1086:1090	the predicted value	1072:1090	the predicted value of 30.054%	1072:1101	The experimental extraction yield under optimal conditions was found to be 30.049%, which is in good agreement with the predicted value of 30.054%.
26212959	5	56	theme	predicted	1076:1084	arg1	value					1086:1090	the predicted value	1072:1090	the predicted value of 30.054%	1072:1101	The experimental extraction yield under optimal conditions was found to be 30.049%, which is in good agreement with the predicted value of 30.054%.
26212959	1	57	theme	pepsin-soluble	218:231	arg1	PSC					243:245	PSC	243:245	PSC	243:245	Response surface methodology (RSM) was used to investigate the effect of extraction-process variables on pepsin-soluble collagen (PSC) from eggshell membrane.
26212959	1	57	theme	pepsin-soluble	218:231	arg1	collagen					233:240	pepsin-soluble collagen	218:240	pepsin-soluble collagen (PSC) from eggshell membrane	218:269	Response surface methodology (RSM) was used to investigate the effect of extraction-process variables on pepsin-soluble collagen (PSC) from eggshell membrane.
26212959	2	58	theme	enzyme	489:494	arg1	concentration					496:508	enzyme concentration	489:508	enzyme concentration (X3: 15-75 U/mg)	489:525	A central composite design (CCD) was employed for experimental design and analysis of the results to obtain the best possible combination of NaOH concentration (X1: 0.4-1.2 mol/l), alkali treatment time (X2: 6-30 h), enzyme concentration (X3: 15-75 U/mg) and hydrolysis time (X4: 12-60 h) for maximum PSC extraction.
26212959	2	59	theme	composite	282:290	arg1	CCD					300:302	CCD	300:302	CCD	300:302	A central composite design (CCD) was employed for experimental design and analysis of the results to obtain the best possible combination of NaOH concentration (X1: 0.4-1.2 mol/l), alkali treatment time (X2: 6-30 h), enzyme concentration (X3: 15-75 U/mg) and hydrolysis time (X4: 12-60 h) for maximum PSC extraction.
26212959	2	59	theme	composite	282:290	arg1	design					292:297	A central composite design	272:297	A central composite design (CCD)	272:303	A central composite design (CCD) was employed for experimental design and analysis of the results to obtain the best possible combination of NaOH concentration (X1: 0.4-1.2 mol/l), alkali treatment time (X2: 6-30 h), enzyme concentration (X3: 15-75 U/mg) and hydrolysis time (X4: 12-60 h) for maximum PSC extraction.
26212959	2	60	dep	concentration	496:508	arg1	X3					511:512	X3	511:512	X3: 15-75 U/mg	511:524	A central composite design (CCD) was employed for experimental design and analysis of the results to obtain the best possible combination of NaOH concentration (X1: 0.4-1.2 mol/l), alkali treatment time (X2: 6-30 h), enzyme concentration (X3: 15-75 U/mg) and hydrolysis time (X4: 12-60 h) for maximum PSC extraction.
26212959	0	61	theme	response	77:84	arg1	RSM					107:109	RSM	107:109	RSM	107:109	Extraction optimization of pepsin-soluble collagen from eggshell membrane by response surface methodology (RSM).
26212959	0	61	theme	response	77:84	arg1	methodology					94:104	response surface methodology	77:104	response surface methodology (RSM)	77:110	Extraction optimization of pepsin-soluble collagen from eggshell membrane by response surface methodology (RSM).
26212959	1	62	from	effect	176:181	arg1	PSC					243:245	PSC	243:245	PSC	243:245	Response surface methodology (RSM) was used to investigate the effect of extraction-process variables on pepsin-soluble collagen (PSC) from eggshell membrane.
26212959	1	62	from	effect	176:181	arg1	collagen					233:240	pepsin-soluble collagen	218:240	pepsin-soluble collagen (PSC) from eggshell membrane	218:269	Response surface methodology (RSM) was used to investigate the effect of extraction-process variables on pepsin-soluble collagen (PSC) from eggshell membrane.
26212959	5	63	theme	%	1101:1101	arg1	value					1086:1090	the predicted value	1072:1090	the predicted value of 30.054%	1072:1101	The experimental extraction yield under optimal conditions was found to be 30.049%, which is in good agreement with the predicted value of 30.054%.
26212959	2	64	theme	alkali	453:458	arg1	time					470:473	alkali treatment time	453:473	alkali treatment time (X2: 6-30 h)	453:486	A central composite design (CCD) was employed for experimental design and analysis of the results to obtain the best possible combination of NaOH concentration (X1: 0.4-1.2 mol/l), alkali treatment time (X2: 6-30 h), enzyme concentration (X3: 15-75 U/mg) and hydrolysis time (X4: 12-60 h) for maximum PSC extraction.
26212959	4	65	theme	h	889:889	arg1	concentration					899:911	enzyme concentration	892:911	enzyme concentration of 50 U/mg	892:922	According to the results, optimum extraction conditions were as follows: NaOH concentration of 0.76 mol/l, alkali treatment time of 18 h, enzyme concentration of 50 U/mg and hydrolysis time of 43.42 h.
26212959	4	65	theme	h	889:889	arg1	concentration					832:844	NaOH concentration	827:844	NaOH concentration of 0.76 mol/l	827:858	According to the results, optimum extraction conditions were as follows: NaOH concentration of 0.76 mol/l, alkali treatment time of 18 h, enzyme concentration of 50 U/mg and hydrolysis time of 43.42 h.
26212959	4	65	theme	h	889:889	arg1	time					939:942	hydrolysis time	928:942	hydrolysis time of 43.42 h	928:953	According to the results, optimum extraction conditions were as follows: NaOH concentration of 0.76 mol/l, alkali treatment time of 18 h, enzyme concentration of 50 U/mg and hydrolysis time of 43.42 h.
26212959	4	65	theme	h	889:889	arg1	time					878:881	alkali treatment time	861:881	alkali treatment time of 18 h	861:889	According to the results, optimum extraction conditions were as follows: NaOH concentration of 0.76 mol/l, alkali treatment time of 18 h, enzyme concentration of 50 U/mg and hydrolysis time of 43.42 h.
26212959	4	66	theme	alkali	861:866	arg1	time					878:881	alkali treatment time	861:881	alkali treatment time of 18 h	861:889	According to the results, optimum extraction conditions were as follows: NaOH concentration of 0.76 mol/l, alkali treatment time of 18 h, enzyme concentration of 50 U/mg and hydrolysis time of 43.42 h.
25336511	0	0	theme	bone	95:98	arg1	implants					100:107	non-metallic bone implants	82:107	non-metallic bone implants	82:107	Inkjet printing of Chitlac-nanosilver--a method to create functional coatings for non-metallic bone implants.
25336511	4	1	theme	inkjet	756:761	arg1	printer					763:769	a drop-on-demand inkjet printer	739:769	a drop-on-demand inkjet printer	739:769	Herein, we report the use of inkjet technology (a drop-on-demand inkjet printer) to deposit functional Chitlac-nAg coatings on the thermoset substrates.
25336511	4	1	theme	inkjet	756:761	arg1	technology					727:736	inkjet technology	720:736	inkjet technology (a drop-on-demand inkjet printer)	720:770	Herein, we report the use of inkjet technology (a drop-on-demand inkjet printer) to deposit functional Chitlac-nAg coatings on the thermoset substrates.
25336511	5	2	theme	Characterization	844:859	arg1	methods					861:867	Characterization methods	844:867	Characterization methods	844:867	Characterization methods included scanning electron microscopy, scanning white light interferometry and electro-thermal atomic absorption spectroscopy.
25336511	1	3	theme	triethyleneglycoldimethacrylate	189:219	arg1	matrix					239:244	bisphenol-A-dimethacrylate and triethyleneglycoldimethacrylate thermoset polymer matrix	158:244	bisphenol-A-dimethacrylate and triethyleneglycoldimethacrylate thermoset polymer matrix reinforced with E-glass fibers	158:275	Biostable fiber-reinforced composites, based on bisphenol-A-dimethacrylate and triethyleneglycoldimethacrylate thermoset polymer matrix reinforced with E-glass fibers have been successfully used in cranial reconstructions and the material has been approved for clinical use.
25336511	0	4	theme	non-metallic	82:93	arg1	implants					100:107	non-metallic bone implants	82:107	non-metallic bone implants	82:107	Inkjet printing of Chitlac-nanosilver--a method to create functional coatings for non-metallic bone implants.
25336511	6	5	theme	flexible	1033:1040	arg1	functionalization					1042:1058	the fast and flexible functionalization	1020:1058	the fast and flexible functionalization of the thermoset surfaces with controlled coating patterns	1020:1117	Inkjet printing enabled the fast and flexible functionalization of the thermoset surfaces with controlled coating patterns.
25336511	7	6	theme	conventional	1256:1267	arg1	technique					1279:1287	conventional immersion technique	1256:1287	conventional immersion technique	1256:1287	The coatings were not impaired by the printing process: the kinetics of silver release from the coatings created by inkjet printing and conventional immersion technique was similar.
25336511	1	7	theme	thermoset	221:229	arg1	matrix					239:244	bisphenol-A-dimethacrylate and triethyleneglycoldimethacrylate thermoset polymer matrix	158:244	bisphenol-A-dimethacrylate and triethyleneglycoldimethacrylate thermoset polymer matrix reinforced with E-glass fibers	158:275	Biostable fiber-reinforced composites, based on bisphenol-A-dimethacrylate and triethyleneglycoldimethacrylate thermoset polymer matrix reinforced with E-glass fibers have been successfully used in cranial reconstructions and the material has been approved for clinical use.
25336511	8	8	theme	Further	1302:1308	arg1	research					1310:1317	Further research	1302:1317	Further research	1302:1317	Further research is foreseen to optimize printing parameters and to tailor the characteristics of the coatings for specific clinical applications.
25336511	7	9	theme	inkjet	1236:1241	arg1	printing					1243:1250	inkjet printing	1236:1250	inkjet printing	1236:1250	The coatings were not impaired by the printing process: the kinetics of silver release from the coatings created by inkjet printing and conventional immersion technique was similar.
25336511	3	10	theme	lactose-modified	615:630	arg1	Chitlac					642:648	Chitlac	642:648	Chitlac	642:648	Silver nanoparticles (nAg) were immobilized in lactose-modified chitosan (Chitlac) to prepare the bacteriostatic coatings.
25336511	3	10	theme	lactose-modified	615:630	arg1	chitosan					632:639	lactose-modified chitosan	615:639	lactose-modified chitosan (Chitlac)	615:649	Silver nanoparticles (nAg) were immobilized in lactose-modified chitosan (Chitlac) to prepare the bacteriostatic coatings.
25336511	4	11	theme	technology	727:736	arg1	use					713:715	the use	709:715	the use of inkjet technology (a drop-on-demand inkjet printer) to deposit functional Chitlac-nAg coatings on the thermoset substrates	709:841	Herein, we report the use of inkjet technology (a drop-on-demand inkjet printer) to deposit functional Chitlac-nAg coatings on the thermoset substrates.
25336511	6	12	theme	fast	1024:1027	arg1	functionalization					1042:1058	the fast and flexible functionalization	1020:1058	the fast and flexible functionalization of the thermoset surfaces with controlled coating patterns	1020:1117	Inkjet printing enabled the fast and flexible functionalization of the thermoset surfaces with controlled coating patterns.
25336511	2	13	theme	implants	418:425	arg1	refinement					398:407	a further refinement	388:407	a further refinement of these implants	388:425	As a further refinement of these implants, antimicrobial, non-cytotoxic coatings on the composites were created by an immersion procedure driven by strong electrostatic interactions.
25336511	2	13	theme	implants	418:425	arg1	coatings					457:464	antimicrobial, non-cytotoxic coatings	428:464	antimicrobial, non-cytotoxic coatings on the composites	428:482	As a further refinement of these implants, antimicrobial, non-cytotoxic coatings on the composites were created by an immersion procedure driven by strong electrostatic interactions.
25336511	7	14	from	kinetics	1180:1187	arg1	coatings					1216:1223	the coatings	1212:1223	the coatings created by inkjet printing and conventional immersion technique	1212:1287	The coatings were not impaired by the printing process: the kinetics of silver release from the coatings created by inkjet printing and conventional immersion technique was similar.
25336511	5	15	theme	absorption	971:980	arg1	spectroscopy					982:993	electro-thermal atomic absorption spectroscopy	948:993	electro-thermal atomic absorption spectroscopy	948:993	Characterization methods included scanning electron microscopy, scanning white light interferometry and electro-thermal atomic absorption spectroscopy.
25336511	6	16	with	functionalization	1042:1058	arg1	patterns					1110:1117	controlled coating patterns	1091:1117	controlled coating patterns	1091:1117	Inkjet printing enabled the fast and flexible functionalization of the thermoset surfaces with controlled coating patterns.
25336511	2	17	dep	antimicrobial	428:440	arg1	non-cytotoxic					443:455	non-cytotoxic	443:455	non-cytotoxic	443:455	As a further refinement of these implants, antimicrobial, non-cytotoxic coatings on the composites were created by an immersion procedure driven by strong electrostatic interactions.
25336511	1	18	theme	polymer	231:237	arg1	matrix					239:244	bisphenol-A-dimethacrylate and triethyleneglycoldimethacrylate thermoset polymer matrix	158:244	bisphenol-A-dimethacrylate and triethyleneglycoldimethacrylate thermoset polymer matrix reinforced with E-glass fibers	158:275	Biostable fiber-reinforced composites, based on bisphenol-A-dimethacrylate and triethyleneglycoldimethacrylate thermoset polymer matrix reinforced with E-glass fibers have been successfully used in cranial reconstructions and the material has been approved for clinical use.
25336511	7	19	theme	immersion	1269:1277	arg1	technique					1279:1287	conventional immersion technique	1256:1287	conventional immersion technique	1256:1287	The coatings were not impaired by the printing process: the kinetics of silver release from the coatings created by inkjet printing and conventional immersion technique was similar.
25336511	0	20	theme	Inkjet	0:5	arg1	printing					7:14	Inkjet printing	0:14	Inkjet printing of Chitlac-nanosilver--a method	0:46	Inkjet printing of Chitlac-nanosilver--a method to create functional coatings for non-metallic bone implants.
25336511	4	21	theme	inkjet	720:725	arg1	printer					763:769	a drop-on-demand inkjet printer	739:769	a drop-on-demand inkjet printer	739:769	Herein, we report the use of inkjet technology (a drop-on-demand inkjet printer) to deposit functional Chitlac-nAg coatings on the thermoset substrates.
25336511	4	21	theme	inkjet	720:725	arg1	technology					727:736	inkjet technology	720:736	inkjet technology (a drop-on-demand inkjet printer)	720:770	Herein, we report the use of inkjet technology (a drop-on-demand inkjet printer) to deposit functional Chitlac-nAg coatings on the thermoset substrates.
25336511	2	22	theme	immersion	503:511	arg1	procedure					513:521	an immersion procedure	500:521	an immersion procedure driven by strong electrostatic interactions	500:565	As a further refinement of these implants, antimicrobial, non-cytotoxic coatings on the composites were created by an immersion procedure driven by strong electrostatic interactions.
25336511	8	23	theme	coatings	1404:1411	arg1	characteristics					1381:1395	the characteristics	1377:1395	the characteristics of the coatings for specific clinical applications	1377:1446	Further research is foreseen to optimize printing parameters and to tailor the characteristics of the coatings for specific clinical applications.
25336511	0	24	theme	Chitlac-nanosilver--a	19:39	arg1	method					41:46	Chitlac-nanosilver--a method	19:46	Chitlac-nanosilver--a method	19:46	Inkjet printing of Chitlac-nanosilver--a method to create functional coatings for non-metallic bone implants.
25336511	5	25	theme	scanning	908:915	arg1	interferometry					929:942	scanning white light interferometry	908:942	scanning white light interferometry	908:942	Characterization methods included scanning electron microscopy, scanning white light interferometry and electro-thermal atomic absorption spectroscopy.
25336511	7	26	theme	release	1199:1205	arg1	similar					1293:1299	similar	1293:1299	similar	1293:1299	The coatings were not impaired by the printing process: the kinetics of silver release from the coatings created by inkjet printing and conventional immersion technique was similar.
25336511	7	26	theme	release	1199:1205	arg1	kinetics					1180:1187	the kinetics	1176:1187	the kinetics of silver release from the coatings created by inkjet printing and conventional immersion technique	1176:1287	The coatings were not impaired by the printing process: the kinetics of silver release from the coatings created by inkjet printing and conventional immersion technique was similar.
25336511	8	27	theme	printing	1343:1350	arg1	parameters					1352:1361	printing parameters	1343:1361	printing parameters	1343:1361	Further research is foreseen to optimize printing parameters and to tailor the characteristics of the coatings for specific clinical applications.
25336511	1	28	theme	Biostable	110:118	arg1	composites					137:146	Biostable fiber-reinforced composites	110:146	Biostable fiber-reinforced composites	110:146	Biostable fiber-reinforced composites, based on bisphenol-A-dimethacrylate and triethyleneglycoldimethacrylate thermoset polymer matrix reinforced with E-glass fibers have been successfully used in cranial reconstructions and the material has been approved for clinical use.
25336511	1	29	theme	E-glass	262:268	arg1	fibers					270:275	E-glass fibers	262:275	E-glass fibers	262:275	Biostable fiber-reinforced composites, based on bisphenol-A-dimethacrylate and triethyleneglycoldimethacrylate thermoset polymer matrix reinforced with E-glass fibers have been successfully used in cranial reconstructions and the material has been approved for clinical use.
25336511	5	30	theme	light	923:927	arg1	interferometry					929:942	scanning white light interferometry	908:942	scanning white light interferometry	908:942	Characterization methods included scanning electron microscopy, scanning white light interferometry and electro-thermal atomic absorption spectroscopy.
25336511	6	31	theme	coating	1102:1108	arg1	patterns					1110:1117	controlled coating patterns	1091:1117	controlled coating patterns	1091:1117	Inkjet printing enabled the fast and flexible functionalization of the thermoset surfaces with controlled coating patterns.
25336511	6	32	theme	Inkjet	996:1001	arg1	printing					1003:1010	Inkjet printing	996:1010	Inkjet printing	996:1010	Inkjet printing enabled the fast and flexible functionalization of the thermoset surfaces with controlled coating patterns.
25336511	1	33	theme	fiber-reinforced	120:135	arg1	composites					137:146	Biostable fiber-reinforced composites	110:146	Biostable fiber-reinforced composites	110:146	Biostable fiber-reinforced composites, based on bisphenol-A-dimethacrylate and triethyleneglycoldimethacrylate thermoset polymer matrix reinforced with E-glass fibers have been successfully used in cranial reconstructions and the material has been approved for clinical use.
25336511	1	34	theme	bisphenol-A-dimethacrylate	158:183	arg1	matrix					239:244	bisphenol-A-dimethacrylate and triethyleneglycoldimethacrylate thermoset polymer matrix	158:244	bisphenol-A-dimethacrylate and triethyleneglycoldimethacrylate thermoset polymer matrix reinforced with E-glass fibers	158:275	Biostable fiber-reinforced composites, based on bisphenol-A-dimethacrylate and triethyleneglycoldimethacrylate thermoset polymer matrix reinforced with E-glass fibers have been successfully used in cranial reconstructions and the material has been approved for clinical use.
25336511	2	35	theme	electrostatic	540:552	arg1	interactions					554:565	strong electrostatic interactions	533:565	strong electrostatic interactions	533:565	As a further refinement of these implants, antimicrobial, non-cytotoxic coatings on the composites were created by an immersion procedure driven by strong electrostatic interactions.
25336511	6	36	theme	controlled	1091:1100	arg1	patterns					1110:1117	controlled coating patterns	1091:1117	controlled coating patterns	1091:1117	Inkjet printing enabled the fast and flexible functionalization of the thermoset surfaces with controlled coating patterns.
25336511	8	37	theme	clinical	1426:1433	arg1	applications					1435:1446	specific clinical applications	1417:1446	specific clinical applications	1417:1446	Further research is foreseen to optimize printing parameters and to tailor the characteristics of the coatings for specific clinical applications.
25336511	3	38	theme	bacteriostatic	666:679	arg1	coatings					681:688	the bacteriostatic coatings	662:688	the bacteriostatic coatings	662:688	Silver nanoparticles (nAg) were immobilized in lactose-modified chitosan (Chitlac) to prepare the bacteriostatic coatings.
25336511	5	39	theme	electro-thermal	948:962	arg1	spectroscopy					982:993	electro-thermal atomic absorption spectroscopy	948:993	electro-thermal atomic absorption spectroscopy	948:993	Characterization methods included scanning electron microscopy, scanning white light interferometry and electro-thermal atomic absorption spectroscopy.
25336511	0	40	theme	method	41:46	arg1	printing					7:14	Inkjet printing	0:14	Inkjet printing of Chitlac-nanosilver--a method	0:46	Inkjet printing of Chitlac-nanosilver--a method to create functional coatings for non-metallic bone implants.
25336511	1	41	theme	clinical	371:378	arg1	use					380:382	clinical use	371:382	clinical use	371:382	Biostable fiber-reinforced composites, based on bisphenol-A-dimethacrylate and triethyleneglycoldimethacrylate thermoset polymer matrix reinforced with E-glass fibers have been successfully used in cranial reconstructions and the material has been approved for clinical use.
25336511	2	42	theme	strong	533:538	arg1	interactions					554:565	strong electrostatic interactions	533:565	strong electrostatic interactions	533:565	As a further refinement of these implants, antimicrobial, non-cytotoxic coatings on the composites were created by an immersion procedure driven by strong electrostatic interactions.
25336511	5	43	theme	white	917:921	arg1	interferometry					929:942	scanning white light interferometry	908:942	scanning white light interferometry	908:942	Characterization methods included scanning electron microscopy, scanning white light interferometry and electro-thermal atomic absorption spectroscopy.
25336511	5	44	theme	atomic	964:969	arg1	spectroscopy					982:993	electro-thermal atomic absorption spectroscopy	948:993	electro-thermal atomic absorption spectroscopy	948:993	Characterization methods included scanning electron microscopy, scanning white light interferometry and electro-thermal atomic absorption spectroscopy.
25336511	0	45	theme	functional	58:67	arg1	coatings					69:76	functional coatings	58:76	functional coatings for non-metallic bone implants	58:107	Inkjet printing of Chitlac-nanosilver--a method to create functional coatings for non-metallic bone implants.
25336511	6	46	theme	surfaces	1077:1084	arg1	functionalization					1042:1058	the fast and flexible functionalization	1020:1058	the fast and flexible functionalization of the thermoset surfaces with controlled coating patterns	1020:1117	Inkjet printing enabled the fast and flexible functionalization of the thermoset surfaces with controlled coating patterns.
25336511	7	47	from	coatings	1216:1223	arg1	similar					1293:1299	similar	1293:1299	similar	1293:1299	The coatings were not impaired by the printing process: the kinetics of silver release from the coatings created by inkjet printing and conventional immersion technique was similar.
25336511	7	47	from	coatings	1216:1223	arg1	kinetics					1180:1187	the kinetics	1176:1187	the kinetics of silver release from the coatings created by inkjet printing and conventional immersion technique	1176:1287	The coatings were not impaired by the printing process: the kinetics of silver release from the coatings created by inkjet printing and conventional immersion technique was similar.
25336511	7	47	from	coatings	1216:1223	arg1	release					1199:1205	silver release	1192:1205	silver release from the coatings created by inkjet printing and conventional immersion technique	1192:1287	The coatings were not impaired by the printing process: the kinetics of silver release from the coatings created by inkjet printing and conventional immersion technique was similar.
25336511	7	48	theme	printing	1158:1165	arg1	process					1167:1173	the printing process	1154:1173	the printing process	1154:1173	The coatings were not impaired by the printing process: the kinetics of silver release from the coatings created by inkjet printing and conventional immersion technique was similar.
25336511	3	49	theme	Silver	568:573	arg1	nAg					590:592	nAg	590:592	nAg	590:592	Silver nanoparticles (nAg) were immobilized in lactose-modified chitosan (Chitlac) to prepare the bacteriostatic coatings.
25336511	3	49	theme	Silver	568:573	arg1	nanoparticles					575:587	Silver nanoparticles	568:587	Silver nanoparticles (nAg)	568:593	Silver nanoparticles (nAg) were immobilized in lactose-modified chitosan (Chitlac) to prepare the bacteriostatic coatings.
25336511	7	50	theme	silver	1192:1197	arg1	release					1199:1205	silver release	1192:1205	silver release from the coatings created by inkjet printing and conventional immersion technique	1192:1287	The coatings were not impaired by the printing process: the kinetics of silver release from the coatings created by inkjet printing and conventional immersion technique was similar.
25336511	6	51	theme	thermoset	1067:1075	arg1	surfaces					1077:1084	the thermoset surfaces	1063:1084	the thermoset surfaces	1063:1084	Inkjet printing enabled the fast and flexible functionalization of the thermoset surfaces with controlled coating patterns.
25336511	2	52	from	coatings	457:464	arg1	composites					473:482	the composites	469:482	the composites	469:482	As a further refinement of these implants, antimicrobial, non-cytotoxic coatings on the composites were created by an immersion procedure driven by strong electrostatic interactions.
25336511	2	53	theme	further	390:396	arg1	refinement					398:407	a further refinement	388:407	a further refinement of these implants	388:425	As a further refinement of these implants, antimicrobial, non-cytotoxic coatings on the composites were created by an immersion procedure driven by strong electrostatic interactions.
25336511	2	53	theme	further	390:396	arg1	coatings					457:464	antimicrobial, non-cytotoxic coatings	428:464	antimicrobial, non-cytotoxic coatings on the composites	428:482	As a further refinement of these implants, antimicrobial, non-cytotoxic coatings on the composites were created by an immersion procedure driven by strong electrostatic interactions.
25336511	4	54	theme	thermoset	822:830	arg1	substrates					832:841	the thermoset substrates	818:841	the thermoset substrates	818:841	Herein, we report the use of inkjet technology (a drop-on-demand inkjet printer) to deposit functional Chitlac-nAg coatings on the thermoset substrates.
25336511	1	55	used	used	300:303	arg2	composites					137:146	Biostable fiber-reinforced composites	110:146	Biostable fiber-reinforced composites	110:146	Biostable fiber-reinforced composites, based on bisphenol-A-dimethacrylate and triethyleneglycoldimethacrylate thermoset polymer matrix reinforced with E-glass fibers have been successfully used in cranial reconstructions and the material has been approved for clinical use.
25336511	4	56	theme	functional	783:792	arg1	coatings					806:813	functional Chitlac-nAg coatings	783:813	functional Chitlac-nAg coatings	783:813	Herein, we report the use of inkjet technology (a drop-on-demand inkjet printer) to deposit functional Chitlac-nAg coatings on the thermoset substrates.
25336511	4	57	theme	Chitlac-nAg	794:804	arg1	coatings					806:813	functional Chitlac-nAg coatings	783:813	functional Chitlac-nAg coatings	783:813	Herein, we report the use of inkjet technology (a drop-on-demand inkjet printer) to deposit functional Chitlac-nAg coatings on the thermoset substrates.
25336511	8	58	theme	specific	1417:1424	arg1	applications					1435:1446	specific clinical applications	1417:1446	specific clinical applications	1417:1446	Further research is foreseen to optimize printing parameters and to tailor the characteristics of the coatings for specific clinical applications.
25336511	2	59	theme	antimicrobial	428:440	arg1	coatings					457:464	antimicrobial, non-cytotoxic coatings	428:464	antimicrobial, non-cytotoxic coatings on the composites	428:482	As a further refinement of these implants, antimicrobial, non-cytotoxic coatings on the composites were created by an immersion procedure driven by strong electrostatic interactions.
25336511	2	59	theme	antimicrobial	428:440	arg1	refinement					398:407	a further refinement	388:407	a further refinement of these implants	388:425	As a further refinement of these implants, antimicrobial, non-cytotoxic coatings on the composites were created by an immersion procedure driven by strong electrostatic interactions.
25336511	5	60	theme	electron	887:894	arg1	microscopy					896:905	scanning electron microscopy	878:905	scanning electron microscopy	878:905	Characterization methods included scanning electron microscopy, scanning white light interferometry and electro-thermal atomic absorption spectroscopy.
25336511	5	61	theme	scanning	878:885	arg1	microscopy					896:905	scanning electron microscopy	878:905	scanning electron microscopy	878:905	Characterization methods included scanning electron microscopy, scanning white light interferometry and electro-thermal atomic absorption spectroscopy.
25336511	4	62	theme	drop-on-demand	741:754	arg1	printer					763:769	a drop-on-demand inkjet printer	739:769	a drop-on-demand inkjet printer	739:769	Herein, we report the use of inkjet technology (a drop-on-demand inkjet printer) to deposit functional Chitlac-nAg coatings on the thermoset substrates.
25336511	4	62	theme	drop-on-demand	741:754	arg1	technology					727:736	inkjet technology	720:736	inkjet technology (a drop-on-demand inkjet printer)	720:770	Herein, we report the use of inkjet technology (a drop-on-demand inkjet printer) to deposit functional Chitlac-nAg coatings on the thermoset substrates.
25336511	1	63	theme	cranial	308:314	arg1	reconstructions					316:330	cranial reconstructions	308:330	cranial reconstructions	308:330	Biostable fiber-reinforced composites, based on bisphenol-A-dimethacrylate and triethyleneglycoldimethacrylate thermoset polymer matrix reinforced with E-glass fibers have been successfully used in cranial reconstructions and the material has been approved for clinical use.
26022969	0	0	theme	biomass	85:91	arg1	types					93:97	biomass types	85:97	biomass types	85:97	Properties of biomass-derived biochars: Combined effects of operating conditions and biomass types.
26022969	3	1	from	time	654:657	arg1	structures					617:626	the biomass structures	605:626	the biomass structures at around 400 °C. Heating time and rate	605:666	The X-ray diffraction data confirmed the occurrence of phase transition in the biomass structures at around 400 °C. Heating time and rate showed little effect on the functional group compositions of the biochars within 8h, particularly under N2 atmosphere.
26022969	3	1	from	time	654:657	arg1	occurrence					571:580	the occurrence	567:580	the occurrence of phase transition in the biomass structures at around 400 °C. Heating time and rate	567:666	The X-ray diffraction data confirmed the occurrence of phase transition in the biomass structures at around 400 °C. Heating time and rate showed little effect on the functional group compositions of the biochars within 8h, particularly under N2 atmosphere.
26022969	4	2	theme	dissolved	845:853	arg1	carbon					863:868	the biochar-derived dissolved organic carbon	825:868	the biochar-derived dissolved organic carbon	825:868	In addition, the molecular weights of the biochar-derived dissolved organic carbon tended to increase with increasing temperature.
26022969	1	3	theme	pine	304:307	arg1	sawdust					309:315	pine sawdust	304:315	pine sawdust	304:315	Combined effects of operating conditions including heating temperature (200-700 °C), time (1-8h) and rate, and atmosphere (air-flow, air-limited and N2) on the physicochemical properties of biochars with pine sawdust, maize straw and sugarcane bagasse as feedstocks were investigated.
26022969	4	4	theme	biochar-derived	829:843	arg1	carbon					863:868	the biochar-derived dissolved organic carbon	825:868	the biochar-derived dissolved organic carbon	825:868	In addition, the molecular weights of the biochar-derived dissolved organic carbon tended to increase with increasing temperature.
26022969	3	5	dep	confirmed	557:565	arg1	showed					668:673	showed	668:673	showed	668:673	The X-ray diffraction data confirmed the occurrence of phase transition in the biomass structures at around 400 °C. Heating time and rate showed little effect on the functional group compositions of the biochars within 8h, particularly under N2 atmosphere.
26022969	1	6	with	biochars	290:297	arg1	sugarcane					334:342	sugarcane	334:342	sugarcane	334:342	Combined effects of operating conditions including heating temperature (200-700 °C), time (1-8h) and rate, and atmosphere (air-flow, air-limited and N2) on the physicochemical properties of biochars with pine sawdust, maize straw and sugarcane bagasse as feedstocks were investigated.
26022969	1	6	with	biochars	290:297	arg1	sawdust					309:315	pine sawdust	304:315	pine sawdust	304:315	Combined effects of operating conditions including heating temperature (200-700 °C), time (1-8h) and rate, and atmosphere (air-flow, air-limited and N2) on the physicochemical properties of biochars with pine sawdust, maize straw and sugarcane bagasse as feedstocks were investigated.
26022969	1	6	with	biochars	290:297	arg1	straw					324:328	maize straw	318:328	maize straw	318:328	Combined effects of operating conditions including heating temperature (200-700 °C), time (1-8h) and rate, and atmosphere (air-flow, air-limited and N2) on the physicochemical properties of biochars with pine sawdust, maize straw and sugarcane bagasse as feedstocks were investigated.
26022969	4	7	theme	carbon	863:868	arg1	weights					814:820	the molecular weights	800:820	the molecular weights of the biochar-derived dissolved organic carbon	800:868	In addition, the molecular weights of the biochar-derived dissolved organic carbon tended to increase with increasing temperature.
26022969	0	8	link	biomass-derived	14:28	arg1	biochars					30:37	biomass-derived biochars	14:37	biomass-derived biochars	14:37	Properties of biomass-derived biochars: Combined effects of operating conditions and biomass types.
26022969	5	9	theme	biochar	951:957	arg1	properties					959:968	the biochar properties	947:968	the biochar properties	947:968	Feedstock type also affected the biochar properties by the compositional differences in mineral salts and cellulose/lignin in the three biomass materials.
26022969	0	10	theme	types	93:97	arg1	effects					49:55	Combined effects	40:55	Properties of biomass-derived biochars: Combined effects of operating conditions and biomass types.	0:98	Properties of biomass-derived biochars: Combined effects of operating conditions and biomass types.
26022969	3	11	theme	functional	696:705	arg1	compositions					713:724	the functional group compositions	692:724	the functional group compositions of the biochars within 8h	692:750	The X-ray diffraction data confirmed the occurrence of phase transition in the biomass structures at around 400 °C. Heating time and rate showed little effect on the functional group compositions of the biochars within 8h, particularly under N2 atmosphere.
26022969	3	12	theme	X-ray	534:538	arg1	data					552:555	The X-ray diffraction data	530:555	The X-ray diffraction data	530:555	The X-ray diffraction data confirmed the occurrence of phase transition in the biomass structures at around 400 °C. Heating time and rate showed little effect on the functional group compositions of the biochars within 8h, particularly under N2 atmosphere.
26022969	4	13	link	biochar-derived	829:843	arg1	carbon					863:868	the biochar-derived dissolved organic carbon	825:868	the biochar-derived dissolved organic carbon	825:868	In addition, the molecular weights of the biochar-derived dissolved organic carbon tended to increase with increasing temperature.
26022969	5	14	from	differences	991:1001	arg1	materials					1062:1070	the three biomass materials	1044:1070	the three biomass materials	1044:1070	Feedstock type also affected the biochar properties by the compositional differences in mineral salts and cellulose/lignin in the three biomass materials.
26022969	5	14	from	differences	991:1001	arg1	cellulose/lignin					1024:1039	cellulose/lignin	1024:1039	cellulose/lignin	1024:1039	Feedstock type also affected the biochar properties by the compositional differences in mineral salts and cellulose/lignin in the three biomass materials.
26022969	5	14	from	differences	991:1001	arg1	salts					1014:1018	mineral salts	1006:1018	mineral salts	1006:1018	Feedstock type also affected the biochar properties by the compositional differences in mineral salts and cellulose/lignin in the three biomass materials.
26022969	1	15	theme	maize	318:322	arg1	straw					324:328	maize straw	318:328	maize straw	318:328	Combined effects of operating conditions including heating temperature (200-700 °C), time (1-8h) and rate, and atmosphere (air-flow, air-limited and N2) on the physicochemical properties of biochars with pine sawdust, maize straw and sugarcane bagasse as feedstocks were investigated.
26022969	3	16	from	rate	663:666	arg1	structures					617:626	the biomass structures	605:626	the biomass structures at around 400 °C. Heating time and rate	605:666	The X-ray diffraction data confirmed the occurrence of phase transition in the biomass structures at around 400 °C. Heating time and rate showed little effect on the functional group compositions of the biochars within 8h, particularly under N2 atmosphere.
26022969	3	16	from	rate	663:666	arg1	occurrence					571:580	the occurrence	567:580	the occurrence of phase transition in the biomass structures at around 400 °C. Heating time and rate	567:666	The X-ray diffraction data confirmed the occurrence of phase transition in the biomass structures at around 400 °C. Heating time and rate showed little effect on the functional group compositions of the biochars within 8h, particularly under N2 atmosphere.
26022969	6	17	theme	desired	1168:1174	arg1	properties					1176:1185	desired properties	1168:1185	desired properties	1168:1185	This work provides important information for optimizing procedures for biochar production with desired properties and high yield.
26022969	6	18	theme	high	1191:1194	arg1	yield					1196:1200	high yield	1191:1200	high yield	1191:1200	This work provides important information for optimizing procedures for biochar production with desired properties and high yield.
26022969	6	19	theme	important	1092:1100	arg1	information					1102:1112	important information	1092:1112	important information for optimizing procedures for biochar production with desired properties and high yield	1092:1200	This work provides important information for optimizing procedures for biochar production with desired properties and high yield.
26022969	2	20	theme	production	415:424	arg1	factors					478:484	the predominant factors	462:484	the predominant factors that determined the properties of biochars	462:527	The results demonstrated that production temperature and atmosphere acted as the predominant factors that determined the properties of biochars.
26022969	2	20	theme	production	415:424	arg1	atmosphere					442:451	atmosphere	442:451	atmosphere	442:451	The results demonstrated that production temperature and atmosphere acted as the predominant factors that determined the properties of biochars.
26022969	2	20	theme	production	415:424	arg1	temperature					426:436	production temperature	415:436	production temperature	415:436	The results demonstrated that production temperature and atmosphere acted as the predominant factors that determined the properties of biochars.
26022969	3	21	theme	group	707:711	arg1	compositions					713:724	the functional group compositions	692:724	the functional group compositions of the biochars within 8h	692:750	The X-ray diffraction data confirmed the occurrence of phase transition in the biomass structures at around 400 °C. Heating time and rate showed little effect on the functional group compositions of the biochars within 8h, particularly under N2 atmosphere.
26022969	0	22	theme	biochars	30:37	arg1	Properties					0:9	Properties	0:9	Properties of biomass-derived biochars: Combined effects of operating conditions and biomass types.	0:98	Properties of biomass-derived biochars: Combined effects of operating conditions and biomass types.
26022969	3	23	theme	biochars	733:740	arg1	compositions					713:724	the functional group compositions	692:724	the functional group compositions of the biochars within 8h	692:750	The X-ray diffraction data confirmed the occurrence of phase transition in the biomass structures at around 400 °C. Heating time and rate showed little effect on the functional group compositions of the biochars within 8h, particularly under N2 atmosphere.
26022969	1	24	theme	Combined	100:107	arg1	effects					109:115	Combined effects	100:115	Combined effects of operating conditions including heating temperature (200-700 °C), time (1-8h) and rate, and atmosphere (air-flow, air-limited and N2) on the physicochemical properties of biochars with pine sawdust, maize straw and sugarcane	100:342	Combined effects of operating conditions including heating temperature (200-700 °C), time (1-8h) and rate, and atmosphere (air-flow, air-limited and N2) on the physicochemical properties of biochars with pine sawdust, maize straw and sugarcane bagasse as feedstocks were investigated.
26022969	3	25	from	occurrence	571:580	arg1	rate					663:666	rate	663:666	rate	663:666	The X-ray diffraction data confirmed the occurrence of phase transition in the biomass structures at around 400 °C. Heating time and rate showed little effect on the functional group compositions of the biochars within 8h, particularly under N2 atmosphere.
26022969	3	25	from	occurrence	571:580	arg1	time					654:657	around 400 °C. Heating time	631:657	around 400 °C. Heating time	631:657	The X-ray diffraction data confirmed the occurrence of phase transition in the biomass structures at around 400 °C. Heating time and rate showed little effect on the functional group compositions of the biochars within 8h, particularly under N2 atmosphere.
26022969	3	25	from	occurrence	571:580	arg1	structures					617:626	the biomass structures	605:626	the biomass structures at around 400 °C. Heating time and rate	605:666	The X-ray diffraction data confirmed the occurrence of phase transition in the biomass structures at around 400 °C. Heating time and rate showed little effect on the functional group compositions of the biochars within 8h, particularly under N2 atmosphere.
26022969	0	26	theme	biomass-derived	14:28	arg1	biochars					30:37	biomass-derived biochars	14:37	biomass-derived biochars	14:37	Properties of biomass-derived biochars: Combined effects of operating conditions and biomass types.
26022969	3	27	theme	diffraction	540:550	arg1	data					552:555	The X-ray diffraction data	530:555	The X-ray diffraction data	530:555	The X-ray diffraction data confirmed the occurrence of phase transition in the biomass structures at around 400 °C. Heating time and rate showed little effect on the functional group compositions of the biochars within 8h, particularly under N2 atmosphere.
26022969	0	28	theme	Combined	40:47	arg1	effects					49:55	Combined effects	40:55	Properties of biomass-derived biochars: Combined effects of operating conditions and biomass types.	0:98	Properties of biomass-derived biochars: Combined effects of operating conditions and biomass types.
26022969	6	29	theme	biochar	1144:1150	arg1	production					1152:1161	biochar production	1144:1161	biochar production	1144:1161	This work provides important information for optimizing procedures for biochar production with desired properties and high yield.
26022969	2	30	theme	biochars	520:527	arg1	properties					506:515	the properties	502:515	the properties of biochars	502:527	The results demonstrated that production temperature and atmosphere acted as the predominant factors that determined the properties of biochars.
26022969	1	31	dep	atmosphere	211:220	arg1	air-flow					223:230	air-flow	223:230	air-flow	223:230	Combined effects of operating conditions including heating temperature (200-700 °C), time (1-8h) and rate, and atmosphere (air-flow, air-limited and N2) on the physicochemical properties of biochars with pine sawdust, maize straw and sugarcane bagasse as feedstocks were investigated.
26022969	1	31	dep	atmosphere	211:220	arg1	N2					249:250	N2	249:250	N2	249:250	Combined effects of operating conditions including heating temperature (200-700 °C), time (1-8h) and rate, and atmosphere (air-flow, air-limited and N2) on the physicochemical properties of biochars with pine sawdust, maize straw and sugarcane bagasse as feedstocks were investigated.
26022969	1	31	dep	atmosphere	211:220	arg1	air-limited					233:243	air-limited	233:243	air-limited	233:243	Combined effects of operating conditions including heating temperature (200-700 °C), time (1-8h) and rate, and atmosphere (air-flow, air-limited and N2) on the physicochemical properties of biochars with pine sawdust, maize straw and sugarcane bagasse as feedstocks were investigated.
26022969	3	32	theme	phase	585:589	arg1	transition					591:600	phase transition	585:600	phase transition	585:600	The X-ray diffraction data confirmed the occurrence of phase transition in the biomass structures at around 400 °C. Heating time and rate showed little effect on the functional group compositions of the biochars within 8h, particularly under N2 atmosphere.
26022969	1	33	theme	physicochemical	260:274	arg1	properties					276:285	the physicochemical properties	256:285	the physicochemical properties of biochars with pine sawdust, maize straw and sugarcane	256:342	Combined effects of operating conditions including heating temperature (200-700 °C), time (1-8h) and rate, and atmosphere (air-flow, air-limited and N2) on the physicochemical properties of biochars with pine sawdust, maize straw and sugarcane bagasse as feedstocks were investigated.
26022969	2	34	theme	predominant	466:476	arg1	factors					478:484	the predominant factors	462:484	the predominant factors that determined the properties of biochars	462:527	The results demonstrated that production temperature and atmosphere acted as the predominant factors that determined the properties of biochars.
26022969	2	34	theme	predominant	466:476	arg1	atmosphere					442:451	atmosphere	442:451	atmosphere	442:451	The results demonstrated that production temperature and atmosphere acted as the predominant factors that determined the properties of biochars.
26022969	2	34	theme	predominant	466:476	arg1	temperature					426:436	production temperature	415:436	production temperature	415:436	The results demonstrated that production temperature and atmosphere acted as the predominant factors that determined the properties of biochars.
26022969	3	35	theme	little	675:680	arg1	effect					682:687	little effect	675:687	little effect	675:687	The X-ray diffraction data confirmed the occurrence of phase transition in the biomass structures at around 400 °C. Heating time and rate showed little effect on the functional group compositions of the biochars within 8h, particularly under N2 atmosphere.
26022969	4	36	theme	molecular	804:812	arg1	weights					814:820	the molecular weights	800:820	the molecular weights of the biochar-derived dissolved organic carbon	800:868	In addition, the molecular weights of the biochar-derived dissolved organic carbon tended to increase with increasing temperature.
26022969	1	37	theme	operating	120:128	arg1	conditions					130:139	operating conditions	120:139	operating conditions including heating temperature (200-700 °C), time (1-8h) and rate, and atmosphere (air-flow, air-limited and N2)	120:251	Combined effects of operating conditions including heating temperature (200-700 °C), time (1-8h) and rate, and atmosphere (air-flow, air-limited and N2) on the physicochemical properties of biochars with pine sawdust, maize straw and sugarcane bagasse as feedstocks were investigated.
26022969	1	37	theme	operating	120:128	arg1	temperature					159:169	heating temperature	151:169	heating temperature (200-700 °C)	151:182	Combined effects of operating conditions including heating temperature (200-700 °C), time (1-8h) and rate, and atmosphere (air-flow, air-limited and N2) on the physicochemical properties of biochars with pine sawdust, maize straw and sugarcane bagasse as feedstocks were investigated.
26022969	1	37	theme	operating	120:128	arg1	atmosphere					211:220	atmosphere	211:220	atmosphere (air-flow, air-limited and N2)	211:251	Combined effects of operating conditions including heating temperature (200-700 °C), time (1-8h) and rate, and atmosphere (air-flow, air-limited and N2) on the physicochemical properties of biochars with pine sawdust, maize straw and sugarcane bagasse as feedstocks were investigated.
26022969	1	37	theme	operating	120:128	arg1	time					185:188	time	185:188	time (1-8h)	185:195	Combined effects of operating conditions including heating temperature (200-700 °C), time (1-8h) and rate, and atmosphere (air-flow, air-limited and N2) on the physicochemical properties of biochars with pine sawdust, maize straw and sugarcane bagasse as feedstocks were investigated.
26022969	5	38	theme	mineral	1006:1012	arg1	salts					1014:1018	mineral salts	1006:1018	mineral salts	1006:1018	Feedstock type also affected the biochar properties by the compositional differences in mineral salts and cellulose/lignin in the three biomass materials.
26022969	5	39	theme	compositional	977:989	arg1	differences					991:1001	the compositional differences	973:1001	the compositional differences in mineral salts and cellulose/lignin in the three biomass materials	973:1070	Feedstock type also affected the biochar properties by the compositional differences in mineral salts and cellulose/lignin in the three biomass materials.
26022969	1	40	theme	conditions	130:139	arg1	effects					109:115	Combined effects	100:115	Combined effects of operating conditions including heating temperature (200-700 °C), time (1-8h) and rate, and atmosphere (air-flow, air-limited and N2) on the physicochemical properties of biochars with pine sawdust, maize straw and sugarcane	100:342	Combined effects of operating conditions including heating temperature (200-700 °C), time (1-8h) and rate, and atmosphere (air-flow, air-limited and N2) on the physicochemical properties of biochars with pine sawdust, maize straw and sugarcane bagasse as feedstocks were investigated.
26022969	1	41	from	effects	109:115	arg1	properties					276:285	the physicochemical properties	256:285	the physicochemical properties of biochars with pine sawdust, maize straw and sugarcane	256:342	Combined effects of operating conditions including heating temperature (200-700 °C), time (1-8h) and rate, and atmosphere (air-flow, air-limited and N2) on the physicochemical properties of biochars with pine sawdust, maize straw and sugarcane bagasse as feedstocks were investigated.
26022969	3	42	theme	°C.	642:644	arg1	time					654:657	around 400 °C. Heating time	631:657	around 400 °C. Heating time	631:657	The X-ray diffraction data confirmed the occurrence of phase transition in the biomass structures at around 400 °C. Heating time and rate showed little effect on the functional group compositions of the biochars within 8h, particularly under N2 atmosphere.
26022969	3	43	theme	biomass	609:615	arg1	structures					617:626	the biomass structures	605:626	the biomass structures at around 400 °C. Heating time and rate	605:666	The X-ray diffraction data confirmed the occurrence of phase transition in the biomass structures at around 400 °C. Heating time and rate showed little effect on the functional group compositions of the biochars within 8h, particularly under N2 atmosphere.
26022969	5	44	theme	Feedstock	918:926	arg1	type					928:931	Feedstock type	918:931	Feedstock type	918:931	Feedstock type also affected the biochar properties by the compositional differences in mineral salts and cellulose/lignin in the three biomass materials.
26022969	4	45	theme	organic	855:861	arg1	carbon					863:868	the biochar-derived dissolved organic carbon	825:868	the biochar-derived dissolved organic carbon	825:868	In addition, the molecular weights of the biochar-derived dissolved organic carbon tended to increase with increasing temperature.
26022969	5	46	theme	biomass	1054:1060	arg1	materials					1062:1070	the three biomass materials	1044:1070	the three biomass materials	1044:1070	Feedstock type also affected the biochar properties by the compositional differences in mineral salts and cellulose/lignin in the three biomass materials.
26022969	0	47	theme	conditions	70:79	arg1	effects					49:55	Combined effects	40:55	Properties of biomass-derived biochars: Combined effects of operating conditions and biomass types.	0:98	Properties of biomass-derived biochars: Combined effects of operating conditions and biomass types.
26022969	3	48	theme	Heating	646:652	arg1	time					654:657	around 400 °C. Heating time	631:657	around 400 °C. Heating time	631:657	The X-ray diffraction data confirmed the occurrence of phase transition in the biomass structures at around 400 °C. Heating time and rate showed little effect on the functional group compositions of the biochars within 8h, particularly under N2 atmosphere.
26022969	3	49	theme	transition	591:600	arg1	occurrence					571:580	the occurrence	567:580	the occurrence of phase transition in the biomass structures at around 400 °C. Heating time and rate	567:666	The X-ray diffraction data confirmed the occurrence of phase transition in the biomass structures at around 400 °C. Heating time and rate showed little effect on the functional group compositions of the biochars within 8h, particularly under N2 atmosphere.
26022969	1	50	theme	heating	151:157	arg1	temperature					159:169	heating temperature	151:169	heating temperature (200-700 °C)	151:182	Combined effects of operating conditions including heating temperature (200-700 °C), time (1-8h) and rate, and atmosphere (air-flow, air-limited and N2) on the physicochemical properties of biochars with pine sawdust, maize straw and sugarcane bagasse as feedstocks were investigated.
26022969	1	50	theme	heating	151:157	arg1	°C					180:181	200-700 °C	172:181	200-700 °C	172:181	Combined effects of operating conditions including heating temperature (200-700 °C), time (1-8h) and rate, and atmosphere (air-flow, air-limited and N2) on the physicochemical properties of biochars with pine sawdust, maize straw and sugarcane bagasse as feedstocks were investigated.
26022969	0	51	theme	operating	60:68	arg1	conditions					70:79	operating conditions	60:79	operating conditions	60:79	Properties of biomass-derived biochars: Combined effects of operating conditions and biomass types.
26022969	1	52	theme	biochars	290:297	arg1	properties					276:285	the physicochemical properties	256:285	the physicochemical properties of biochars with pine sawdust, maize straw and sugarcane	256:342	Combined effects of operating conditions including heating temperature (200-700 °C), time (1-8h) and rate, and atmosphere (air-flow, air-limited and N2) on the physicochemical properties of biochars with pine sawdust, maize straw and sugarcane bagasse as feedstocks were investigated.
26022969	0	53	dep	Properties	0:9	arg1	effects					49:55	Combined effects	40:55	Properties of biomass-derived biochars: Combined effects of operating conditions and biomass types.	0:98	Properties of biomass-derived biochars: Combined effects of operating conditions and biomass types.
26022969	3	54	theme	N2	772:773	arg1	atmosphere					775:784	N2 atmosphere	772:784	N2 atmosphere	772:784	The X-ray diffraction data confirmed the occurrence of phase transition in the biomass structures at around 400 °C. Heating time and rate showed little effect on the functional group compositions of the biochars within 8h, particularly under N2 atmosphere.
26389872	3	0	from	mg/mL	814:818	arg1	preincubation					779:791	preincubation	779:791	preincubation of the bacteria at 1 mg/mL	779:818	Ammonium sulfate precipitation of an aqueous extract yielded two fractions at 60% and 90% saturation with significant antiadhesive effects against H. pylori, strain J99, (FE60% 68% ± 15%; FE90% 75% ± 11% inhibition rates) after preincubation of the bacteria at 1 mg/mL.
26389872	3	1	theme	%	730:730	arg1	saturation					641:650	90% saturation	637:650	90% saturation with significant antiadhesive effects against H. pylori	637:706	Ammonium sulfate precipitation of an aqueous extract yielded two fractions at 60% and 90% saturation with significant antiadhesive effects against H. pylori, strain J99, (FE60% 68% ± 15%; FE90% 75% ± 11% inhibition rates) after preincubation of the bacteria at 1 mg/mL.
26389872	3	1	theme	%	730:730	arg1	%					736:736	FE60% 68% ± 15%	722:736	FE60% 68% ± 15%	722:736	Ammonium sulfate precipitation of an aqueous extract yielded two fractions at 60% and 90% saturation with significant antiadhesive effects against H. pylori, strain J99, (FE60% 68% ± 15%; FE90% 75% ± 11% inhibition rates) after preincubation of the bacteria at 1 mg/mL.
26389872	6	2	theme	complementary	1152:1164	arg1	ligands					1144:1150	ligands	1144:1150	ligands complementary to BabA and SabA	1144:1181	A reduction of bacterial binding to ligands complementary to BabA and SabA was observed when bacteria were pretreated with FE90%.
26389872	3	3	theme	aqueous	588:594	arg1	extract					596:602	an aqueous extract	585:602	an aqueous extract	585:602	Ammonium sulfate precipitation of an aqueous extract yielded two fractions at 60% and 90% saturation with significant antiadhesive effects against H. pylori, strain J99, (FE60% 68% ± 15%; FE90% 75% ± 11% inhibition rates) after preincubation of the bacteria at 1 mg/mL.
26389872	3	4	theme	%	726:726	arg1	saturation					641:650	90% saturation	637:650	90% saturation with significant antiadhesive effects against H. pylori	637:706	Ammonium sulfate precipitation of an aqueous extract yielded two fractions at 60% and 90% saturation with significant antiadhesive effects against H. pylori, strain J99, (FE60% 68% ± 15%; FE90% 75% ± 11% inhibition rates) after preincubation of the bacteria at 1 mg/mL.
26389872	3	4	theme	%	726:726	arg1	%					736:736	FE60% 68% ± 15%	722:736	FE60% 68% ± 15%	722:736	Ammonium sulfate precipitation of an aqueous extract yielded two fractions at 60% and 90% saturation with significant antiadhesive effects against H. pylori, strain J99, (FE60% 68% ± 15%; FE90% 75% ± 11% inhibition rates) after preincubation of the bacteria at 1 mg/mL.
26389872	8	5	theme	antiadhesive	1628:1639	arg1	activity					1641:1648	antiadhesive activity	1628:1648	antiadhesive activity	1628:1648	Deacetylation of HBSS and FE90% resulted in loss of the antiadhesive activity, indicating esterification being a prerequisite for antiadhesive activity.
26389872	1	6	theme	Polysaccharide	190:203	arg1	extracts					216:223	Polysaccharide containing extracts	190:223	Polysaccharide containing extracts from immature fruits of okra (Abelmoschus esculentus)	190:277	Polysaccharide containing extracts from immature fruits of okra (Abelmoschus esculentus) are known to exhibit antiadhesive effects against bacterial adhesion of Helicobacter pylori (H. pylori) to stomach tissue.
26389872	7	7	theme	molecular	1295:1303	arg1	polysaccharides					1278:1292	the antiadhesive polysaccharides	1261:1292	the antiadhesive polysaccharides (molecular weight, monomer composition, linkage analysis, stereochemistry, and acetylation)	1261:1384	Structural analysis of the antiadhesive polysaccharides (molecular weight, monomer composition, linkage analysis, stereochemistry, and acetylation) indicated the presence of acetylated rhamnogalacturonan-I polymers, decorated with short galactose side chains.
26389872	7	7	theme	molecular	1295:1303	arg1	weight					1305:1310	molecular weight	1295:1310	molecular weight	1295:1310	Structural analysis of the antiadhesive polysaccharides (molecular weight, monomer composition, linkage analysis, stereochemistry, and acetylation) indicated the presence of acetylated rhamnogalacturonan-I polymers, decorated with short galactose side chains.
26389872	6	8	theme	bacterial	1123:1131	arg1	binding					1133:1139	bacterial binding	1123:1139	bacterial binding to ligands complementary to BabA and SabA	1123:1181	A reduction of bacterial binding to ligands complementary to BabA and SabA was observed when bacteria were pretreated with FE90%.
26389872	4	9	theme	buffer	870:875	arg1	HBSS					893:896	HBSS	893:896	HBSS	893:896	Sequential extraction of okra fruits yielded hot buffer soluble solids (HBSS) with dose dependent antiadhesive effects against strain J99 and three clinical isolates.
26389872	4	9	theme	buffer	870:875	arg1	solids					885:890	hot buffer soluble solids	866:890	hot buffer soluble solids (HBSS)	866:897	Sequential extraction of okra fruits yielded hot buffer soluble solids (HBSS) with dose dependent antiadhesive effects against strain J99 and three clinical isolates.
26389872	7	10	theme	polysaccharides	1278:1292	arg1	analysis					1249:1256	Structural analysis	1238:1256	Structural analysis of the antiadhesive polysaccharides (molecular weight, monomer composition, linkage analysis, stereochemistry, and acetylation)	1238:1384	Structural analysis of the antiadhesive polysaccharides (molecular weight, monomer composition, linkage analysis, stereochemistry, and acetylation) indicated the presence of acetylated rhamnogalacturonan-I polymers, decorated with short galactose side chains.
26389872	0	11	theme	Gastric	131:137	arg1	Cells					139:143	Human Gastric Cells	125:143	Human Gastric Cells	125:143	Acetylated Rhamnogalacturonans from Immature Fruits of Abelmoschus esculentus Inhibit the Adhesion of Helicobacter pylori to Human Gastric Cells by Interaction with Outer Membrane Proteins.
26389872	1	12	theme	immature	230:237	arg1	fruits					239:244	immature fruits	230:244	immature fruits of okra (Abelmoschus esculentus)	230:277	Polysaccharide containing extracts from immature fruits of okra (Abelmoschus esculentus) are known to exhibit antiadhesive effects against bacterial adhesion of Helicobacter pylori (H. pylori) to stomach tissue.
26389872	3	13	theme	%	747:747	arg1	rates					766:770	FE90% 75% ± 11% inhibition rates	739:770	FE90% 75% ± 11% inhibition rates	739:770	Ammonium sulfate precipitation of an aqueous extract yielded two fractions at 60% and 90% saturation with significant antiadhesive effects against H. pylori, strain J99, (FE60% 68% ± 15%; FE90% 75% ± 11% inhibition rates) after preincubation of the bacteria at 1 mg/mL.
26389872	1	14	from	fruits	239:244	arg1	extracts					216:223	Polysaccharide containing extracts	190:223	Polysaccharide containing extracts from immature fruits of okra (Abelmoschus esculentus)	190:277	Polysaccharide containing extracts from immature fruits of okra (Abelmoschus esculentus) are known to exhibit antiadhesive effects against bacterial adhesion of Helicobacter pylori (H. pylori) to stomach tissue.
26389872	4	15	theme	okra	846:849	arg1	fruits					851:856	okra fruits	846:856	okra fruits	846:856	Sequential extraction of okra fruits yielded hot buffer soluble solids (HBSS) with dose dependent antiadhesive effects against strain J99 and three clinical isolates.
26389872	3	16	theme	%	743:743	arg1	rates					766:770	FE90% 75% ± 11% inhibition rates	739:770	FE90% 75% ± 11% inhibition rates	739:770	Ammonium sulfate precipitation of an aqueous extract yielded two fractions at 60% and 90% saturation with significant antiadhesive effects against H. pylori, strain J99, (FE60% 68% ± 15%; FE90% 75% ± 11% inhibition rates) after preincubation of the bacteria at 1 mg/mL.
26389872	3	17	theme	inhibition	755:764	arg1	rates					766:770	FE90% 75% ± 11% inhibition rates	739:770	FE90% 75% ± 11% inhibition rates	739:770	Ammonium sulfate precipitation of an aqueous extract yielded two fractions at 60% and 90% saturation with significant antiadhesive effects against H. pylori, strain J99, (FE60% 68% ± 15%; FE90% 75% ± 11% inhibition rates) after preincubation of the bacteria at 1 mg/mL.
26389872	0	18	theme	Membrane	171:178	arg1	Proteins					180:187	Outer Membrane Proteins	165:187	Outer Membrane Proteins	165:187	Acetylated Rhamnogalacturonans from Immature Fruits of Abelmoschus esculentus Inhibit the Adhesion of Helicobacter pylori to Human Gastric Cells by Interaction with Outer Membrane Proteins.
26389872	5	19	theme	H.	1005:1006	arg1	pylori					1008:1013	H. pylori	1005:1013	H. pylori	1005:1013	Preincubation of H. pylori with HBSS (1 mg/mL) led to reduced binding to 3'-sialyl lactose, sialylated Le(a) and Le(x).
26389872	4	20	theme	dependent	909:917	arg1	effects					932:938	dose dependent antiadhesive effects	904:938	dose dependent antiadhesive effects against strain J99 and three clinical isolates	904:985	Sequential extraction of okra fruits yielded hot buffer soluble solids (HBSS) with dose dependent antiadhesive effects against strain J99 and three clinical isolates.
26389872	5	21	theme	reduced	1042:1048	arg1	binding					1050:1056	reduced binding	1042:1056	reduced binding to 3'-sialyl lactose, sialylated Le(a) and Le(x)	1042:1105	Preincubation of H. pylori with HBSS (1 mg/mL) led to reduced binding to 3'-sialyl lactose, sialylated Le(a) and Le(x).
26389872	3	22	theme	%	639:639	arg1	saturation					641:650	90% saturation	637:650	90% saturation with significant antiadhesive effects against H. pylori	637:706	Ammonium sulfate precipitation of an aqueous extract yielded two fractions at 60% and 90% saturation with significant antiadhesive effects against H. pylori, strain J99, (FE60% 68% ± 15%; FE90% 75% ± 11% inhibition rates) after preincubation of the bacteria at 1 mg/mL.
26389872	3	22	theme	%	639:639	arg1	%					736:736	FE60% 68% ± 15%	722:736	FE60% 68% ± 15%	722:736	Ammonium sulfate precipitation of an aqueous extract yielded two fractions at 60% and 90% saturation with significant antiadhesive effects against H. pylori, strain J99, (FE60% 68% ± 15%; FE90% 75% ± 11% inhibition rates) after preincubation of the bacteria at 1 mg/mL.
26389872	3	22	theme	%	639:639	arg1	strain					709:714	strain J99	709:718	strain J99	709:718	Ammonium sulfate precipitation of an aqueous extract yielded two fractions at 60% and 90% saturation with significant antiadhesive effects against H. pylori, strain J99, (FE60% 68% ± 15%; FE90% 75% ± 11% inhibition rates) after preincubation of the bacteria at 1 mg/mL.
26389872	2	23	theme	attachment	525:534	arg1	inhibition					501:510	this inhibition	496:510	this inhibition of bacterial attachment to host cells	496:548	The present study investigates structural and functional features of polymers responsible for this inhibition of bacterial attachment to host cells.
26389872	5	24	gly	sialylated	1080:1089	arg1	a					1094:1094	a	1094:1094	a	1094:1094	Preincubation of H. pylori with HBSS (1 mg/mL) led to reduced binding to 3'-sialyl lactose, sialylated Le(a) and Le(x).
26389872	5	24	gly	sialylated	1080:1089	arg1	lactose					1071:1077	3'-sialyl lactose	1061:1077	3'-sialyl lactose	1061:1077	Preincubation of H. pylori with HBSS (1 mg/mL) led to reduced binding to 3'-sialyl lactose, sialylated Le(a) and Le(x).
26389872	5	24	gly	sialylated	1080:1089	arg1	Le					1091:1092	sialylated Le	1080:1092	sialylated Le(a)	1080:1095	Preincubation of H. pylori with HBSS (1 mg/mL) led to reduced binding to 3'-sialyl lactose, sialylated Le(a) and Le(x).
26389872	7	25	theme	galactose	1475:1483	arg1	chains					1490:1495	short galactose side chains	1469:1495	short galactose side chains	1469:1495	Structural analysis of the antiadhesive polysaccharides (molecular weight, monomer composition, linkage analysis, stereochemistry, and acetylation) indicated the presence of acetylated rhamnogalacturonan-I polymers, decorated with short galactose side chains.
26389872	0	26	theme	Helicobacter	102:113	arg1	pylori					115:120	Helicobacter pylori	102:120	Helicobacter pylori	102:120	Acetylated Rhamnogalacturonans from Immature Fruits of Abelmoschus esculentus Inhibit the Adhesion of Helicobacter pylori to Human Gastric Cells by Interaction with Outer Membrane Proteins.
26389872	1	27	theme	stomach	386:392	arg1	tissue					394:399	stomach tissue	386:399	stomach tissue	386:399	Polysaccharide containing extracts from immature fruits of okra (Abelmoschus esculentus) are known to exhibit antiadhesive effects against bacterial adhesion of Helicobacter pylori (H. pylori) to stomach tissue.
26389872	4	28	theme	clinical	969:976	arg1	isolates					978:985	three clinical isolates	963:985	three clinical isolates	963:985	Sequential extraction of okra fruits yielded hot buffer soluble solids (HBSS) with dose dependent antiadhesive effects against strain J99 and three clinical isolates.
26389872	0	29	from	Fruits	45:50	arg1	Rhamnogalacturonans					11:29	Acetylated Rhamnogalacturonans	0:29	Acetylated Rhamnogalacturonans from Immature Fruits of Abelmoschus esculentus	0:76	Acetylated Rhamnogalacturonans from Immature Fruits of Abelmoschus esculentus Inhibit the Adhesion of Helicobacter pylori to Human Gastric Cells by Interaction with Outer Membrane Proteins.
26389872	2	30	theme	host	539:542	arg1	cells					544:548	host cells	539:548	host cells	539:548	The present study investigates structural and functional features of polymers responsible for this inhibition of bacterial attachment to host cells.
26389872	0	31	theme	Acetylated	0:9	arg1	Rhamnogalacturonans					11:29	Acetylated Rhamnogalacturonans	0:29	Acetylated Rhamnogalacturonans from Immature Fruits of Abelmoschus esculentus	0:76	Acetylated Rhamnogalacturonans from Immature Fruits of Abelmoschus esculentus Inhibit the Adhesion of Helicobacter pylori to Human Gastric Cells by Interaction with Outer Membrane Proteins.
26389872	2	32	theme	functional	448:457	arg1	features					459:466	structural and functional features	433:466	structural and functional features of polymers responsible for this inhibition of bacterial attachment to host cells	433:548	The present study investigates structural and functional features of polymers responsible for this inhibition of bacterial attachment to host cells.
26389872	7	33	theme	polymers	1444:1451	arg1	presence					1400:1407	the presence	1396:1407	the presence of acetylated rhamnogalacturonan-I polymers, decorated with short galactose side chains	1396:1495	Structural analysis of the antiadhesive polysaccharides (molecular weight, monomer composition, linkage analysis, stereochemistry, and acetylation) indicated the presence of acetylated rhamnogalacturonan-I polymers, decorated with short galactose side chains.
26389872	2	34	theme	structural	433:442	arg1	features					459:466	structural and functional features	433:466	structural and functional features of polymers responsible for this inhibition of bacterial attachment to host cells	433:548	The present study investigates structural and functional features of polymers responsible for this inhibition of bacterial attachment to host cells.
26389872	1	35	theme	bacterial	329:337	arg1	adhesion					339:346	bacterial adhesion	329:346	bacterial adhesion of Helicobacter pylori (H. pylori) to stomach tissue	329:399	Polysaccharide containing extracts from immature fruits of okra (Abelmoschus esculentus) are known to exhibit antiadhesive effects against bacterial adhesion of Helicobacter pylori (H. pylori) to stomach tissue.
26389872	7	36	theme	acetylated	1412:1421	arg1	polymers					1444:1451	acetylated rhamnogalacturonan-I polymers	1412:1451	acetylated rhamnogalacturonan-I polymers	1412:1451	Structural analysis of the antiadhesive polysaccharides (molecular weight, monomer composition, linkage analysis, stereochemistry, and acetylation) indicated the presence of acetylated rhamnogalacturonan-I polymers, decorated with short galactose side chains.
26389872	3	37	theme	antiadhesive	669:680	arg1	effects					682:688	significant antiadhesive effects	657:688	significant antiadhesive effects against H. pylori	657:706	Ammonium sulfate precipitation of an aqueous extract yielded two fractions at 60% and 90% saturation with significant antiadhesive effects against H. pylori, strain J99, (FE60% 68% ± 15%; FE90% 75% ± 11% inhibition rates) after preincubation of the bacteria at 1 mg/mL.
26389872	5	38	theme	sialylated	1080:1089	arg1	a					1094:1094	a	1094:1094	a	1094:1094	Preincubation of H. pylori with HBSS (1 mg/mL) led to reduced binding to 3'-sialyl lactose, sialylated Le(a) and Le(x).
26389872	5	38	theme	sialylated	1080:1089	arg1	lactose					1071:1077	3'-sialyl lactose	1061:1077	3'-sialyl lactose	1061:1077	Preincubation of H. pylori with HBSS (1 mg/mL) led to reduced binding to 3'-sialyl lactose, sialylated Le(a) and Le(x).
26389872	5	38	theme	sialylated	1080:1089	arg1	Le					1091:1092	sialylated Le	1080:1092	sialylated Le(a)	1080:1095	Preincubation of H. pylori with HBSS (1 mg/mL) led to reduced binding to 3'-sialyl lactose, sialylated Le(a) and Le(x).
26389872	1	39	theme	pylori	364:369	arg1	adhesion					339:346	bacterial adhesion	329:346	bacterial adhesion of Helicobacter pylori (H. pylori) to stomach tissue	329:399	Polysaccharide containing extracts from immature fruits of okra (Abelmoschus esculentus) are known to exhibit antiadhesive effects against bacterial adhesion of Helicobacter pylori (H. pylori) to stomach tissue.
26389872	1	40	theme	okra	249:252	arg1	fruits					239:244	immature fruits	230:244	immature fruits of okra (Abelmoschus esculentus)	230:277	Polysaccharide containing extracts from immature fruits of okra (Abelmoschus esculentus) are known to exhibit antiadhesive effects against bacterial adhesion of Helicobacter pylori (H. pylori) to stomach tissue.
26389872	3	41	theme	Ammonium	551:558	arg1	precipitation					568:580	Ammonium sulfate precipitation	551:580	Ammonium sulfate precipitation of an aqueous extract	551:602	Ammonium sulfate precipitation of an aqueous extract yielded two fractions at 60% and 90% saturation with significant antiadhesive effects against H. pylori, strain J99, (FE60% 68% ± 15%; FE90% 75% ± 11% inhibition rates) after preincubation of the bacteria at 1 mg/mL.
26389872	8	42	theme	antiadhesive	1554:1565	arg1	activity					1567:1574	the antiadhesive activity	1550:1574	the antiadhesive activity	1550:1574	Deacetylation of HBSS and FE90% resulted in loss of the antiadhesive activity, indicating esterification being a prerequisite for antiadhesive activity.
26389872	3	43	dep	H.	698:699	arg1	pylori					701:706	H. pylori	698:706	H. pylori	698:706	Ammonium sulfate precipitation of an aqueous extract yielded two fractions at 60% and 90% saturation with significant antiadhesive effects against H. pylori, strain J99, (FE60% 68% ± 15%; FE90% 75% ± 11% inhibition rates) after preincubation of the bacteria at 1 mg/mL.
26389872	8	44	theme	%	1528:1528	arg1	Deacetylation					1498:1510	Deacetylation	1498:1510	Deacetylation of HBSS and FE90%	1498:1528	Deacetylation of HBSS and FE90% resulted in loss of the antiadhesive activity, indicating esterification being a prerequisite for antiadhesive activity.
26389872	4	45	theme	Sequential	821:830	arg1	extraction					832:841	Sequential extraction	821:841	Sequential extraction of okra fruits	821:856	Sequential extraction of okra fruits yielded hot buffer soluble solids (HBSS) with dose dependent antiadhesive effects against strain J99 and three clinical isolates.
26389872	3	46	theme	±	732:732	arg1	saturation					641:650	90% saturation	637:650	90% saturation with significant antiadhesive effects against H. pylori	637:706	Ammonium sulfate precipitation of an aqueous extract yielded two fractions at 60% and 90% saturation with significant antiadhesive effects against H. pylori, strain J99, (FE60% 68% ± 15%; FE90% 75% ± 11% inhibition rates) after preincubation of the bacteria at 1 mg/mL.
26389872	3	46	theme	±	732:732	arg1	%					736:736	FE60% 68% ± 15%	722:736	FE60% 68% ± 15%	722:736	Ammonium sulfate precipitation of an aqueous extract yielded two fractions at 60% and 90% saturation with significant antiadhesive effects against H. pylori, strain J99, (FE60% 68% ± 15%; FE90% 75% ± 11% inhibition rates) after preincubation of the bacteria at 1 mg/mL.
26389872	7	47	theme	linkage	1334:1340	arg1	polysaccharides					1278:1292	the antiadhesive polysaccharides	1261:1292	the antiadhesive polysaccharides (molecular weight, monomer composition, linkage analysis, stereochemistry, and acetylation)	1261:1384	Structural analysis of the antiadhesive polysaccharides (molecular weight, monomer composition, linkage analysis, stereochemistry, and acetylation) indicated the presence of acetylated rhamnogalacturonan-I polymers, decorated with short galactose side chains.
26389872	7	47	theme	linkage	1334:1340	arg1	analysis					1342:1349	linkage analysis	1334:1349	linkage analysis	1334:1349	Structural analysis of the antiadhesive polysaccharides (molecular weight, monomer composition, linkage analysis, stereochemistry, and acetylation) indicated the presence of acetylated rhamnogalacturonan-I polymers, decorated with short galactose side chains.
26389872	7	48	theme	monomer	1313:1319	arg1	polysaccharides					1278:1292	the antiadhesive polysaccharides	1261:1292	the antiadhesive polysaccharides (molecular weight, monomer composition, linkage analysis, stereochemistry, and acetylation)	1261:1384	Structural analysis of the antiadhesive polysaccharides (molecular weight, monomer composition, linkage analysis, stereochemistry, and acetylation) indicated the presence of acetylated rhamnogalacturonan-I polymers, decorated with short galactose side chains.
26389872	7	48	theme	monomer	1313:1319	arg1	composition					1321:1331	monomer composition	1313:1331	monomer composition	1313:1331	Structural analysis of the antiadhesive polysaccharides (molecular weight, monomer composition, linkage analysis, stereochemistry, and acetylation) indicated the presence of acetylated rhamnogalacturonan-I polymers, decorated with short galactose side chains.
26389872	4	49	theme	antiadhesive	919:930	arg1	effects					932:938	dose dependent antiadhesive effects	904:938	dose dependent antiadhesive effects against strain J99 and three clinical isolates	904:985	Sequential extraction of okra fruits yielded hot buffer soluble solids (HBSS) with dose dependent antiadhesive effects against strain J99 and three clinical isolates.
26389872	6	50	theme	binding	1133:1139	arg1	reduction					1110:1118	A reduction	1108:1118	A reduction of bacterial binding to ligands complementary to BabA and SabA	1108:1181	A reduction of bacterial binding to ligands complementary to BabA and SabA was observed when bacteria were pretreated with FE90%.
26389872	1	51	theme	containing	205:214	arg1	extracts					216:223	Polysaccharide containing extracts	190:223	Polysaccharide containing extracts from immature fruits of okra (Abelmoschus esculentus)	190:277	Polysaccharide containing extracts from immature fruits of okra (Abelmoschus esculentus) are known to exhibit antiadhesive effects against bacterial adhesion of Helicobacter pylori (H. pylori) to stomach tissue.
26389872	3	52	theme	FE90	739:742	arg1	rates					766:770	FE90% 75% ± 11% inhibition rates	739:770	FE90% 75% ± 11% inhibition rates	739:770	Ammonium sulfate precipitation of an aqueous extract yielded two fractions at 60% and 90% saturation with significant antiadhesive effects against H. pylori, strain J99, (FE60% 68% ± 15%; FE90% 75% ± 11% inhibition rates) after preincubation of the bacteria at 1 mg/mL.
26389872	3	53	theme	extract	596:602	arg1	precipitation					568:580	Ammonium sulfate precipitation	551:580	Ammonium sulfate precipitation of an aqueous extract	551:602	Ammonium sulfate precipitation of an aqueous extract yielded two fractions at 60% and 90% saturation with significant antiadhesive effects against H. pylori, strain J99, (FE60% 68% ± 15%; FE90% 75% ± 11% inhibition rates) after preincubation of the bacteria at 1 mg/mL.
26389872	4	54	theme	hot	866:868	arg1	HBSS					893:896	HBSS	893:896	HBSS	893:896	Sequential extraction of okra fruits yielded hot buffer soluble solids (HBSS) with dose dependent antiadhesive effects against strain J99 and three clinical isolates.
26389872	4	54	theme	hot	866:868	arg1	solids					885:890	hot buffer soluble solids	866:890	hot buffer soluble solids (HBSS)	866:897	Sequential extraction of okra fruits yielded hot buffer soluble solids (HBSS) with dose dependent antiadhesive effects against strain J99 and three clinical isolates.
26389872	4	55	theme	soluble	877:883	arg1	HBSS					893:896	HBSS	893:896	HBSS	893:896	Sequential extraction of okra fruits yielded hot buffer soluble solids (HBSS) with dose dependent antiadhesive effects against strain J99 and three clinical isolates.
26389872	4	55	theme	soluble	877:883	arg1	solids					885:890	hot buffer soluble solids	866:890	hot buffer soluble solids (HBSS)	866:897	Sequential extraction of okra fruits yielded hot buffer soluble solids (HBSS) with dose dependent antiadhesive effects against strain J99 and three clinical isolates.
26389872	1	56	dep	okra	249:252	arg1	esculentus					267:276	Abelmoschus esculentus	255:276	Abelmoschus esculentus	255:276	Polysaccharide containing extracts from immature fruits of okra (Abelmoschus esculentus) are known to exhibit antiadhesive effects against bacterial adhesion of Helicobacter pylori (H. pylori) to stomach tissue.
26389872	7	57	theme	antiadhesive	1265:1276	arg1	weight					1305:1310	molecular weight	1295:1310	molecular weight	1295:1310	Structural analysis of the antiadhesive polysaccharides (molecular weight, monomer composition, linkage analysis, stereochemistry, and acetylation) indicated the presence of acetylated rhamnogalacturonan-I polymers, decorated with short galactose side chains.
26389872	7	57	theme	antiadhesive	1265:1276	arg1	analysis					1342:1349	linkage analysis	1334:1349	linkage analysis	1334:1349	Structural analysis of the antiadhesive polysaccharides (molecular weight, monomer composition, linkage analysis, stereochemistry, and acetylation) indicated the presence of acetylated rhamnogalacturonan-I polymers, decorated with short galactose side chains.
26389872	7	57	theme	antiadhesive	1265:1276	arg1	stereochemistry					1352:1366	stereochemistry	1352:1366	stereochemistry	1352:1366	Structural analysis of the antiadhesive polysaccharides (molecular weight, monomer composition, linkage analysis, stereochemistry, and acetylation) indicated the presence of acetylated rhamnogalacturonan-I polymers, decorated with short galactose side chains.
26389872	7	57	theme	antiadhesive	1265:1276	arg1	composition					1321:1331	monomer composition	1313:1331	monomer composition	1313:1331	Structural analysis of the antiadhesive polysaccharides (molecular weight, monomer composition, linkage analysis, stereochemistry, and acetylation) indicated the presence of acetylated rhamnogalacturonan-I polymers, decorated with short galactose side chains.
26389872	7	57	theme	antiadhesive	1265:1276	arg1	polysaccharides					1278:1292	the antiadhesive polysaccharides	1261:1292	the antiadhesive polysaccharides (molecular weight, monomer composition, linkage analysis, stereochemistry, and acetylation)	1261:1384	Structural analysis of the antiadhesive polysaccharides (molecular weight, monomer composition, linkage analysis, stereochemistry, and acetylation) indicated the presence of acetylated rhamnogalacturonan-I polymers, decorated with short galactose side chains.
26389872	7	57	theme	antiadhesive	1265:1276	arg1	acetylation					1373:1383	acetylation	1373:1383	acetylation	1373:1383	Structural analysis of the antiadhesive polysaccharides (molecular weight, monomer composition, linkage analysis, stereochemistry, and acetylation) indicated the presence of acetylated rhamnogalacturonan-I polymers, decorated with short galactose side chains.
26389872	3	58	theme	±	749:749	arg1	rates					766:770	FE90% 75% ± 11% inhibition rates	739:770	FE90% 75% ± 11% inhibition rates	739:770	Ammonium sulfate precipitation of an aqueous extract yielded two fractions at 60% and 90% saturation with significant antiadhesive effects against H. pylori, strain J99, (FE60% 68% ± 15%; FE90% 75% ± 11% inhibition rates) after preincubation of the bacteria at 1 mg/mL.
26389872	0	59	theme	Outer	165:169	arg1	Proteins					180:187	Outer Membrane Proteins	165:187	Outer Membrane Proteins	165:187	Acetylated Rhamnogalacturonans from Immature Fruits of Abelmoschus esculentus Inhibit the Adhesion of Helicobacter pylori to Human Gastric Cells by Interaction with Outer Membrane Proteins.
26389872	0	60	with	Interaction	148:158	arg1	Proteins					180:187	Outer Membrane Proteins	165:187	Outer Membrane Proteins	165:187	Acetylated Rhamnogalacturonans from Immature Fruits of Abelmoschus esculentus Inhibit the Adhesion of Helicobacter pylori to Human Gastric Cells by Interaction with Outer Membrane Proteins.
26389872	4	61	theme	fruits	851:856	arg1	extraction					832:841	Sequential extraction	821:841	Sequential extraction of okra fruits	821:856	Sequential extraction of okra fruits yielded hot buffer soluble solids (HBSS) with dose dependent antiadhesive effects against strain J99 and three clinical isolates.
26389872	7	62	theme	Structural	1238:1247	arg1	analysis					1249:1256	Structural analysis	1238:1256	Structural analysis of the antiadhesive polysaccharides (molecular weight, monomer composition, linkage analysis, stereochemistry, and acetylation)	1238:1384	Structural analysis of the antiadhesive polysaccharides (molecular weight, monomer composition, linkage analysis, stereochemistry, and acetylation) indicated the presence of acetylated rhamnogalacturonan-I polymers, decorated with short galactose side chains.
26389872	5	63	theme	pylori	1008:1013	arg1	Preincubation					988:1000	Preincubation	988:1000	Preincubation of H. pylori with HBSS (1 mg/mL)	988:1033	Preincubation of H. pylori with HBSS (1 mg/mL) led to reduced binding to 3'-sialyl lactose, sialylated Le(a) and Le(x).
26389872	3	64	theme	90	637:638	arg1	%					639:639	%	639:639	%	639:639	Ammonium sulfate precipitation of an aqueous extract yielded two fractions at 60% and 90% saturation with significant antiadhesive effects against H. pylori, strain J99, (FE60% 68% ± 15%; FE90% 75% ± 11% inhibition rates) after preincubation of the bacteria at 1 mg/mL.
26389872	7	65	dep	polysaccharides	1278:1292	arg1	weight					1305:1310	molecular weight	1295:1310	molecular weight	1295:1310	Structural analysis of the antiadhesive polysaccharides (molecular weight, monomer composition, linkage analysis, stereochemistry, and acetylation) indicated the presence of acetylated rhamnogalacturonan-I polymers, decorated with short galactose side chains.
26389872	7	65	dep	polysaccharides	1278:1292	arg1	analysis					1342:1349	linkage analysis	1334:1349	linkage analysis	1334:1349	Structural analysis of the antiadhesive polysaccharides (molecular weight, monomer composition, linkage analysis, stereochemistry, and acetylation) indicated the presence of acetylated rhamnogalacturonan-I polymers, decorated with short galactose side chains.
26389872	7	65	dep	polysaccharides	1278:1292	arg1	stereochemistry					1352:1366	stereochemistry	1352:1366	stereochemistry	1352:1366	Structural analysis of the antiadhesive polysaccharides (molecular weight, monomer composition, linkage analysis, stereochemistry, and acetylation) indicated the presence of acetylated rhamnogalacturonan-I polymers, decorated with short galactose side chains.
26389872	7	65	dep	polysaccharides	1278:1292	arg1	composition					1321:1331	monomer composition	1313:1331	monomer composition	1313:1331	Structural analysis of the antiadhesive polysaccharides (molecular weight, monomer composition, linkage analysis, stereochemistry, and acetylation) indicated the presence of acetylated rhamnogalacturonan-I polymers, decorated with short galactose side chains.
26389872	7	65	dep	polysaccharides	1278:1292	arg1	polysaccharides					1278:1292	the antiadhesive polysaccharides	1261:1292	the antiadhesive polysaccharides (molecular weight, monomer composition, linkage analysis, stereochemistry, and acetylation)	1261:1384	Structural analysis of the antiadhesive polysaccharides (molecular weight, monomer composition, linkage analysis, stereochemistry, and acetylation) indicated the presence of acetylated rhamnogalacturonan-I polymers, decorated with short galactose side chains.
26389872	7	65	dep	polysaccharides	1278:1292	arg1	acetylation					1373:1383	acetylation	1373:1383	acetylation	1373:1383	Structural analysis of the antiadhesive polysaccharides (molecular weight, monomer composition, linkage analysis, stereochemistry, and acetylation) indicated the presence of acetylated rhamnogalacturonan-I polymers, decorated with short galactose side chains.
26389872	3	66	theme	bacteria	800:807	arg1	preincubation					779:791	preincubation	779:791	preincubation of the bacteria at 1 mg/mL	779:818	Ammonium sulfate precipitation of an aqueous extract yielded two fractions at 60% and 90% saturation with significant antiadhesive effects against H. pylori, strain J99, (FE60% 68% ± 15%; FE90% 75% ± 11% inhibition rates) after preincubation of the bacteria at 1 mg/mL.
26389872	7	67	theme	side	1485:1488	arg1	chains					1490:1495	short galactose side chains	1469:1495	short galactose side chains	1469:1495	Structural analysis of the antiadhesive polysaccharides (molecular weight, monomer composition, linkage analysis, stereochemistry, and acetylation) indicated the presence of acetylated rhamnogalacturonan-I polymers, decorated with short galactose side chains.
26389872	3	68	theme	%	753:753	arg1	rates					766:770	FE90% 75% ± 11% inhibition rates	739:770	FE90% 75% ± 11% inhibition rates	739:770	Ammonium sulfate precipitation of an aqueous extract yielded two fractions at 60% and 90% saturation with significant antiadhesive effects against H. pylori, strain J99, (FE60% 68% ± 15%; FE90% 75% ± 11% inhibition rates) after preincubation of the bacteria at 1 mg/mL.
26389872	3	69	with	saturation	641:650	arg1	effects					682:688	significant antiadhesive effects	657:688	significant antiadhesive effects against H. pylori	657:706	Ammonium sulfate precipitation of an aqueous extract yielded two fractions at 60% and 90% saturation with significant antiadhesive effects against H. pylori, strain J99, (FE60% 68% ± 15%; FE90% 75% ± 11% inhibition rates) after preincubation of the bacteria at 1 mg/mL.
26389872	3	70	dep	%	736:736	arg1	rates					766:770	FE90% 75% ± 11% inhibition rates	739:770	FE90% 75% ± 11% inhibition rates	739:770	Ammonium sulfate precipitation of an aqueous extract yielded two fractions at 60% and 90% saturation with significant antiadhesive effects against H. pylori, strain J99, (FE60% 68% ± 15%; FE90% 75% ± 11% inhibition rates) after preincubation of the bacteria at 1 mg/mL.
26389872	7	71	theme	short	1469:1473	arg1	chains					1490:1495	short galactose side chains	1469:1495	short galactose side chains	1469:1495	Structural analysis of the antiadhesive polysaccharides (molecular weight, monomer composition, linkage analysis, stereochemistry, and acetylation) indicated the presence of acetylated rhamnogalacturonan-I polymers, decorated with short galactose side chains.
26389872	2	72	theme	bacterial	515:523	arg1	attachment					525:534	bacterial attachment	515:534	bacterial attachment to host cells	515:548	The present study investigates structural and functional features of polymers responsible for this inhibition of bacterial attachment to host cells.
26389872	0	73	theme	pylori	115:120	arg1	Adhesion					90:97	the Adhesion	86:97	the Adhesion of Helicobacter pylori to Human Gastric Cells	86:143	Acetylated Rhamnogalacturonans from Immature Fruits of Abelmoschus esculentus Inhibit the Adhesion of Helicobacter pylori to Human Gastric Cells by Interaction with Outer Membrane Proteins.
26389872	5	74	theme	3'-sialyl	1061:1069	arg1	lactose					1071:1077	3'-sialyl lactose	1061:1077	3'-sialyl lactose	1061:1077	Preincubation of H. pylori with HBSS (1 mg/mL) led to reduced binding to 3'-sialyl lactose, sialylated Le(a) and Le(x).
26389872	5	74	theme	3'-sialyl	1061:1069	arg1	Le					1091:1092	sialylated Le	1080:1092	sialylated Le(a)	1080:1095	Preincubation of H. pylori with HBSS (1 mg/mL) led to reduced binding to 3'-sialyl lactose, sialylated Le(a) and Le(x).
26389872	5	74	theme	3'-sialyl	1061:1069	arg1	Le					1101:1102	Le	1101:1102	Le(x)	1101:1105	Preincubation of H. pylori with HBSS (1 mg/mL) led to reduced binding to 3'-sialyl lactose, sialylated Le(a) and Le(x).
26389872	0	75	theme	Human	125:129	arg1	Cells					139:143	Human Gastric Cells	125:143	Human Gastric Cells	125:143	Acetylated Rhamnogalacturonans from Immature Fruits of Abelmoschus esculentus Inhibit the Adhesion of Helicobacter pylori to Human Gastric Cells by Interaction with Outer Membrane Proteins.
26389872	5	76	with	Preincubation	988:1000	arg1	HBSS					1020:1023	HBSS	1020:1023	HBSS (1 mg/mL)	1020:1033	Preincubation of H. pylori with HBSS (1 mg/mL) led to reduced binding to 3'-sialyl lactose, sialylated Le(a) and Le(x).
26389872	5	76	with	Preincubation	988:1000	arg1	mg/mL					1028:1032	1 mg/mL	1026:1032	1 mg/mL	1026:1032	Preincubation of H. pylori with HBSS (1 mg/mL) led to reduced binding to 3'-sialyl lactose, sialylated Le(a) and Le(x).
26389872	3	77	with	%	631:631	arg1	effects					682:688	significant antiadhesive effects	657:688	significant antiadhesive effects against H. pylori	657:706	Ammonium sulfate precipitation of an aqueous extract yielded two fractions at 60% and 90% saturation with significant antiadhesive effects against H. pylori, strain J99, (FE60% 68% ± 15%; FE90% 75% ± 11% inhibition rates) after preincubation of the bacteria at 1 mg/mL.
26389872	2	78	theme	polymers	471:478	arg1	features					459:466	structural and functional features	433:466	structural and functional features of polymers responsible for this inhibition of bacterial attachment to host cells	433:548	The present study investigates structural and functional features of polymers responsible for this inhibition of bacterial attachment to host cells.
26389872	1	79	dep	H.	372:373	arg1	pylori					375:380	H. pylori	372:380	H. pylori	372:380	Polysaccharide containing extracts from immature fruits of okra (Abelmoschus esculentus) are known to exhibit antiadhesive effects against bacterial adhesion of Helicobacter pylori (H. pylori) to stomach tissue.
26389872	0	80	theme	Immature	36:43	arg1	Fruits					45:50	Immature Fruits	36:50	Immature Fruits of Abelmoschus esculentus	36:76	Acetylated Rhamnogalacturonans from Immature Fruits of Abelmoschus esculentus Inhibit the Adhesion of Helicobacter pylori to Human Gastric Cells by Interaction with Outer Membrane Proteins.
26389872	1	81	theme	antiadhesive	300:311	arg1	effects					313:319	antiadhesive effects	300:319	antiadhesive effects	300:319	Polysaccharide containing extracts from immature fruits of okra (Abelmoschus esculentus) are known to exhibit antiadhesive effects against bacterial adhesion of Helicobacter pylori (H. pylori) to stomach tissue.
26389872	3	82	theme	significant	657:667	arg1	effects					682:688	significant antiadhesive effects	657:688	significant antiadhesive effects against H. pylori	657:706	Ammonium sulfate precipitation of an aqueous extract yielded two fractions at 60% and 90% saturation with significant antiadhesive effects against H. pylori, strain J99, (FE60% 68% ± 15%; FE90% 75% ± 11% inhibition rates) after preincubation of the bacteria at 1 mg/mL.
26389872	0	83	theme	esculentus	67:76	arg1	Fruits					45:50	Immature Fruits	36:50	Immature Fruits of Abelmoschus esculentus	36:76	Acetylated Rhamnogalacturonans from Immature Fruits of Abelmoschus esculentus Inhibit the Adhesion of Helicobacter pylori to Human Gastric Cells by Interaction with Outer Membrane Proteins.
26389872	7	84	theme	rhamnogalacturonan-I	1423:1442	arg1	polymers					1444:1451	acetylated rhamnogalacturonan-I polymers	1412:1451	acetylated rhamnogalacturonan-I polymers	1412:1451	Structural analysis of the antiadhesive polysaccharides (molecular weight, monomer composition, linkage analysis, stereochemistry, and acetylation) indicated the presence of acetylated rhamnogalacturonan-I polymers, decorated with short galactose side chains.
26389872	8	85	theme	HBSS	1515:1518	arg1	Deacetylation					1498:1510	Deacetylation	1498:1510	Deacetylation of HBSS and FE90%	1498:1528	Deacetylation of HBSS and FE90% resulted in loss of the antiadhesive activity, indicating esterification being a prerequisite for antiadhesive activity.
26389872	3	86	theme	sulfate	560:566	arg1	precipitation					568:580	Ammonium sulfate precipitation	551:580	Ammonium sulfate precipitation of an aqueous extract	551:602	Ammonium sulfate precipitation of an aqueous extract yielded two fractions at 60% and 90% saturation with significant antiadhesive effects against H. pylori, strain J99, (FE60% 68% ± 15%; FE90% 75% ± 11% inhibition rates) after preincubation of the bacteria at 1 mg/mL.
26389872	8	87	theme	activity	1567:1574	arg1	loss					1542:1545	loss	1542:1545	loss of the antiadhesive activity	1542:1574	Deacetylation of HBSS and FE90% resulted in loss of the antiadhesive activity, indicating esterification being a prerequisite for antiadhesive activity.
26389872	2	88	theme	present	406:412	arg1	study					414:418	The present study	402:418	The present study	402:418	The present study investigates structural and functional features of polymers responsible for this inhibition of bacterial attachment to host cells.
26389872	2	89	theme	responsible	480:490	arg1	polymers					471:478	polymers	471:478	polymers responsible for this inhibition of bacterial attachment to host cells	471:548	The present study investigates structural and functional features of polymers responsible for this inhibition of bacterial attachment to host cells.
26256374	6	0	from	rats	979:982	arg1	implantation					947:958	implantation	947:958	implantation of the hydrogel in rats	947:982	In vivo biocompatibility and biodegradability of the hydrogel were validated by implantation of the hydrogel in rats.
26256374	1	1	theme	physical	183:190	arg1	XG					214:215	XG	214:215	XG	214:215	Injectable hydrogel precursor solution was prepared by physical blend of xanthan gum (XG) and methylcellulose (MC) in aqueous solution.
26256374	1	1	theme	physical	183:190	arg1	blend					192:196	physical blend	183:196	physical blend of xanthan gum (XG)	183:216	Injectable hydrogel precursor solution was prepared by physical blend of xanthan gum (XG) and methylcellulose (MC) in aqueous solution.
26256374	1	2	theme	aqueous	246:252	arg1	solution					254:261	aqueous solution	246:261	aqueous solution	246:261	Injectable hydrogel precursor solution was prepared by physical blend of xanthan gum (XG) and methylcellulose (MC) in aqueous solution.
26256374	2	3	from	temperature	429:439	arg1	property					412:419	good shear-thinning property	392:419	good shear-thinning property at room temperature	392:439	Due to the formation of XG network composed of XG double helical strand structure, XG/MC blend was a high viscous solution with good shear-thinning property at room temperature.
26256374	7	4	theme	promising	1043:1051	arg1	blend					1032:1036	XG/MC blend	1026:1036	XG/MC blend	1026:1036	In vitro investigation demonstrated that XG/MC blend is a promising injectable hydrogel material for long-term drug delivery.
26256374	7	4	theme	promising	1043:1051	arg1	material					1073:1080	a promising injectable hydrogel material	1041:1080	a promising injectable hydrogel material for long-term drug delivery	1041:1108	In vitro investigation demonstrated that XG/MC blend is a promising injectable hydrogel material for long-term drug delivery.
26256374	7	5	theme	drug	1096:1099	arg1	delivery					1101:1108	long-term drug delivery	1086:1108	long-term drug delivery	1086:1108	In vitro investigation demonstrated that XG/MC blend is a promising injectable hydrogel material for long-term drug delivery.
26256374	5	6	theme	in	676:677	arg1	investigations					697:710	Both in vitro and in vivo investigations	671:710	Both in vitro and in vivo investigations	671:710	Both in vitro and in vivo investigations revealed that the blend solution immediately recovered its high viscosity and rapidly formed hydrogel at body temperature after injection using a syringe.
26256374	0	7	with	networks	89:96	arg1	property					118:125	shear-thinning property	103:125	shear-thinning property	103:125	Injectable thermo-responsive hydrogel composed of xanthan gum and methylcellulose double networks with shear-thinning property.
26256374	2	8	theme	XG	288:289	arg1	network					291:297	XG network	288:297	XG network composed of XG double helical strand structure	288:344	Due to the formation of XG network composed of XG double helical strand structure, XG/MC blend was a high viscous solution with good shear-thinning property at room temperature.
26256374	0	9	theme	shear-thinning	103:116	arg1	property					118:125	shear-thinning property	103:125	shear-thinning property	103:125	Injectable thermo-responsive hydrogel composed of xanthan gum and methylcellulose double networks with shear-thinning property.
26256374	7	10	theme	long-term	1086:1094	arg1	delivery					1101:1108	long-term drug delivery	1086:1108	long-term drug delivery	1086:1108	In vitro investigation demonstrated that XG/MC blend is a promising injectable hydrogel material for long-term drug delivery.
26256374	6	11	dep	In	867:868	arg1	vivo					870:873	vivo	870:873	vivo	870:873	In vivo biocompatibility and biodegradability of the hydrogel were validated by implantation of the hydrogel in rats.
26256374	7	12	dep	In	985:986	arg1	vitro					988:992	vitro	988:992	vitro	988:992	In vitro investigation demonstrated that XG/MC blend is a promising injectable hydrogel material for long-term drug delivery.
26256374	1	13	theme	hydrogel	139:146	arg1	solution					158:165	Injectable hydrogel precursor solution	128:165	Injectable hydrogel precursor solution	128:165	Injectable hydrogel precursor solution was prepared by physical blend of xanthan gum (XG) and methylcellulose (MC) in aqueous solution.
26256374	7	14	theme	hydrogel	1064:1071	arg1	blend					1032:1036	XG/MC blend	1026:1036	XG/MC blend	1026:1036	In vitro investigation demonstrated that XG/MC blend is a promising injectable hydrogel material for long-term drug delivery.
26256374	7	14	theme	hydrogel	1064:1071	arg1	material					1073:1080	a promising injectable hydrogel material	1041:1080	a promising injectable hydrogel material for long-term drug delivery	1041:1108	In vitro investigation demonstrated that XG/MC blend is a promising injectable hydrogel material for long-term drug delivery.
26256374	5	15	theme	body	817:820	arg1	temperature					822:832	body temperature	817:832	body temperature after injection using a syringe	817:864	Both in vitro and in vivo investigations revealed that the blend solution immediately recovered its high viscosity and rapidly formed hydrogel at body temperature after injection using a syringe.
26256374	3	16	theme	thermo-responsive	493:509	arg1	network					514:520	thermo-responsive MC network	493:520	thermo-responsive MC network	493:520	When the temperature was changed from 23 to 37 °C, thermo-responsive MC network formed, which caused XG/MC blend solution to gelate.
26256374	2	17	theme	viscous	370:376	arg1	solution					378:385	a high viscous solution	363:385	a high viscous solution with good shear-thinning property at room temperature	363:439	Due to the formation of XG network composed of XG double helical strand structure, XG/MC blend was a high viscous solution with good shear-thinning property at room temperature.
26256374	2	17	theme	viscous	370:376	arg1	blend					353:357	XG/MC blend	347:357	XG/MC blend	347:357	Due to the formation of XG network composed of XG double helical strand structure, XG/MC blend was a high viscous solution with good shear-thinning property at room temperature.
26256374	5	18	theme	in	689:690	arg1	investigations					697:710	Both in vitro and in vivo investigations	671:710	Both in vitro and in vivo investigations	671:710	Both in vitro and in vivo investigations revealed that the blend solution immediately recovered its high viscosity and rapidly formed hydrogel at body temperature after injection using a syringe.
26256374	6	19	theme	hydrogel	967:974	arg1	implantation					947:958	implantation	947:958	implantation of the hydrogel in rats	947:982	In vivo biocompatibility and biodegradability of the hydrogel were validated by implantation of the hydrogel in rats.
26256374	4	20	theme	storage	597:603	arg1	modulus					605:611	storage modulus	597:611	storage modulus	597:611	The gelation time and storage modulus of the blend can be tuned by XG and/or MC concentrations.
26256374	2	21	theme	room	424:427	arg1	temperature					429:439	room temperature	424:439	room temperature	424:439	Due to the formation of XG network composed of XG double helical strand structure, XG/MC blend was a high viscous solution with good shear-thinning property at room temperature.
26256374	1	22	theme	xanthan	201:207	arg1	gum					209:211	xanthan gum	201:211	xanthan gum	201:211	Injectable hydrogel precursor solution was prepared by physical blend of xanthan gum (XG) and methylcellulose (MC) in aqueous solution.
26256374	0	23	theme	thermo-responsive	11:27	arg1	hydrogel					29:36	Injectable thermo-responsive hydrogel	0:36	Injectable thermo-responsive hydrogel	0:36	Injectable thermo-responsive hydrogel composed of xanthan gum and methylcellulose double networks with shear-thinning property.
26256374	6	24	theme	hydrogel	920:927	arg1	biodegradability					896:911	biodegradability	896:911	biodegradability	896:911	In vivo biocompatibility and biodegradability of the hydrogel were validated by implantation of the hydrogel in rats.
26256374	6	24	theme	hydrogel	920:927	arg1	biocompatibility					875:890	biocompatibility	875:890	biocompatibility	875:890	In vivo biocompatibility and biodegradability of the hydrogel were validated by implantation of the hydrogel in rats.
26256374	6	25	from	hydrogel	967:974	arg1	rats					979:982	rats	979:982	rats	979:982	In vivo biocompatibility and biodegradability of the hydrogel were validated by implantation of the hydrogel in rats.
26256374	6	26	theme	In	867:868	arg1	biocompatibility					875:890	biocompatibility	875:890	biocompatibility	875:890	In vivo biocompatibility and biodegradability of the hydrogel were validated by implantation of the hydrogel in rats.
26256374	2	27	with	solution	378:385	arg1	property					412:419	good shear-thinning property	392:419	good shear-thinning property at room temperature	392:439	Due to the formation of XG network composed of XG double helical strand structure, XG/MC blend was a high viscous solution with good shear-thinning property at room temperature.
26256374	1	28	theme	gum	209:211	arg1	methylcellulose					222:236	methylcellulose	222:236	methylcellulose (MC)	222:241	Injectable hydrogel precursor solution was prepared by physical blend of xanthan gum (XG) and methylcellulose (MC) in aqueous solution.
26256374	1	28	theme	gum	209:211	arg1	MC					239:240	MC	239:240	MC	239:240	Injectable hydrogel precursor solution was prepared by physical blend of xanthan gum (XG) and methylcellulose (MC) in aqueous solution.
26256374	1	28	theme	gum	209:211	arg1	XG					214:215	XG	214:215	XG	214:215	Injectable hydrogel precursor solution was prepared by physical blend of xanthan gum (XG) and methylcellulose (MC) in aqueous solution.
26256374	1	28	theme	gum	209:211	arg1	blend					192:196	physical blend	183:196	physical blend of xanthan gum (XG)	183:216	Injectable hydrogel precursor solution was prepared by physical blend of xanthan gum (XG) and methylcellulose (MC) in aqueous solution.
26256374	0	29	theme	Injectable	0:9	arg1	hydrogel					29:36	Injectable thermo-responsive hydrogel	0:36	Injectable thermo-responsive hydrogel	0:36	Injectable thermo-responsive hydrogel composed of xanthan gum and methylcellulose double networks with shear-thinning property.
26256374	7	30	theme	injectable	1053:1062	arg1	blend					1032:1036	XG/MC blend	1026:1036	XG/MC blend	1026:1036	In vitro investigation demonstrated that XG/MC blend is a promising injectable hydrogel material for long-term drug delivery.
26256374	7	30	theme	injectable	1053:1062	arg1	material					1073:1080	a promising injectable hydrogel material	1041:1080	a promising injectable hydrogel material for long-term drug delivery	1041:1108	In vitro investigation demonstrated that XG/MC blend is a promising injectable hydrogel material for long-term drug delivery.
26256374	3	31	theme	XG/MC	543:547	arg1	solution					555:562	XG/MC blend solution	543:562	XG/MC blend solution	543:562	When the temperature was changed from 23 to 37 °C, thermo-responsive MC network formed, which caused XG/MC blend solution to gelate.
26256374	5	32	theme	high	771:774	arg1	viscosity					776:784	its high viscosity	767:784	its high viscosity	767:784	Both in vitro and in vivo investigations revealed that the blend solution immediately recovered its high viscosity and rapidly formed hydrogel at body temperature after injection using a syringe.
26256374	2	33	theme	network	291:297	arg1	formation					275:283	the formation	271:283	the formation of XG network composed of XG double helical strand structure	271:344	Due to the formation of XG network composed of XG double helical strand structure, XG/MC blend was a high viscous solution with good shear-thinning property at room temperature.
26256374	2	34	theme	shear-thinning	397:410	arg1	property					412:419	good shear-thinning property	392:419	good shear-thinning property at room temperature	392:439	Due to the formation of XG network composed of XG double helical strand structure, XG/MC blend was a high viscous solution with good shear-thinning property at room temperature.
26256374	3	35	dep	37	486:487	arg1	to					483:484	to	483:484	to	483:484	When the temperature was changed from 23 to 37 °C, thermo-responsive MC network formed, which caused XG/MC blend solution to gelate.
26256374	1	36	theme	Injectable	128:137	arg1	solution					158:165	Injectable hydrogel precursor solution	128:165	Injectable hydrogel precursor solution	128:165	Injectable hydrogel precursor solution was prepared by physical blend of xanthan gum (XG) and methylcellulose (MC) in aqueous solution.
26256374	2	37	theme	good	392:395	arg1	property					412:419	good shear-thinning property	392:419	good shear-thinning property at room temperature	392:439	Due to the formation of XG network composed of XG double helical strand structure, XG/MC blend was a high viscous solution with good shear-thinning property at room temperature.
26256374	0	38	theme	xanthan	50:56	arg1	gum					58:60	xanthan gum	50:60	xanthan gum	50:60	Injectable thermo-responsive hydrogel composed of xanthan gum and methylcellulose double networks with shear-thinning property.
26256374	2	39	theme	helical	321:327	arg1	structure					336:344	XG double helical strand structure	311:344	XG double helical strand structure	311:344	Due to the formation of XG network composed of XG double helical strand structure, XG/MC blend was a high viscous solution with good shear-thinning property at room temperature.
26256374	7	40	theme	XG/MC	1026:1030	arg1	blend					1032:1036	XG/MC blend	1026:1036	XG/MC blend	1026:1036	In vitro investigation demonstrated that XG/MC blend is a promising injectable hydrogel material for long-term drug delivery.
26256374	7	40	theme	XG/MC	1026:1030	arg1	material					1073:1080	a promising injectable hydrogel material	1041:1080	a promising injectable hydrogel material for long-term drug delivery	1041:1108	In vitro investigation demonstrated that XG/MC blend is a promising injectable hydrogel material for long-term drug delivery.
26256374	4	41	theme	blend	620:624	arg1	modulus					605:611	storage modulus	597:611	storage modulus	597:611	The gelation time and storage modulus of the blend can be tuned by XG and/or MC concentrations.
26256374	4	41	theme	blend	620:624	arg1	time					588:591	gelation time	579:591	gelation time	579:591	The gelation time and storage modulus of the blend can be tuned by XG and/or MC concentrations.
26256374	5	42	theme	blend	730:734	arg1	solution					736:743	the blend solution	726:743	the blend solution	726:743	Both in vitro and in vivo investigations revealed that the blend solution immediately recovered its high viscosity and rapidly formed hydrogel at body temperature after injection using a syringe.
26256374	3	43	theme	blend	549:553	arg1	solution					555:562	XG/MC blend solution	543:562	XG/MC blend solution	543:562	When the temperature was changed from 23 to 37 °C, thermo-responsive MC network formed, which caused XG/MC blend solution to gelate.
26256374	7	44	theme	In	985:986	arg1	investigation					994:1006	In vitro investigation	985:1006	In vitro investigation	985:1006	In vitro investigation demonstrated that XG/MC blend is a promising injectable hydrogel material for long-term drug delivery.
26256374	2	45	theme	strand	329:334	arg1	structure					336:344	XG double helical strand structure	311:344	XG double helical strand structure	311:344	Due to the formation of XG network composed of XG double helical strand structure, XG/MC blend was a high viscous solution with good shear-thinning property at room temperature.
26256374	5	46	dep	in	689:690	arg1	vivo					692:695	vivo	692:695	vivo	692:695	Both in vitro and in vivo investigations revealed that the blend solution immediately recovered its high viscosity and rapidly formed hydrogel at body temperature after injection using a syringe.
26256374	4	47	dep	time	588:591	arg1	The					575:577	The	575:577	The	575:577	The gelation time and storage modulus of the blend can be tuned by XG and/or MC concentrations.
26256374	6	48	from	implantation	947:958	arg1	rats					979:982	rats	979:982	rats	979:982	In vivo biocompatibility and biodegradability of the hydrogel were validated by implantation of the hydrogel in rats.
26256374	1	49	theme	precursor	148:156	arg1	solution					158:165	Injectable hydrogel precursor solution	128:165	Injectable hydrogel precursor solution	128:165	Injectable hydrogel precursor solution was prepared by physical blend of xanthan gum (XG) and methylcellulose (MC) in aqueous solution.
26256374	5	50	dep	in	676:677	arg1	vitro					679:683	vitro	679:683	vitro	679:683	Both in vitro and in vivo investigations revealed that the blend solution immediately recovered its high viscosity and rapidly formed hydrogel at body temperature after injection using a syringe.
26256374	2	51	theme	double	314:319	arg1	structure					336:344	XG double helical strand structure	311:344	XG double helical strand structure	311:344	Due to the formation of XG network composed of XG double helical strand structure, XG/MC blend was a high viscous solution with good shear-thinning property at room temperature.
26256374	3	52	theme	MC	511:512	arg1	network					514:520	thermo-responsive MC network	493:520	thermo-responsive MC network	493:520	When the temperature was changed from 23 to 37 °C, thermo-responsive MC network formed, which caused XG/MC blend solution to gelate.
26256374	2	53	theme	XG	311:312	arg1	structure					336:344	XG double helical strand structure	311:344	XG double helical strand structure	311:344	Due to the formation of XG network composed of XG double helical strand structure, XG/MC blend was a high viscous solution with good shear-thinning property at room temperature.
26256374	0	54	theme	double	82:87	arg1	networks					89:96	methylcellulose double networks	66:96	methylcellulose double networks with shear-thinning property	66:125	Injectable thermo-responsive hydrogel composed of xanthan gum and methylcellulose double networks with shear-thinning property.
26256374	0	55	with	gum	58:60	arg1	property					118:125	shear-thinning property	103:125	shear-thinning property	103:125	Injectable thermo-responsive hydrogel composed of xanthan gum and methylcellulose double networks with shear-thinning property.
26256374	2	56	theme	high	365:368	arg1	solution					378:385	a high viscous solution	363:385	a high viscous solution with good shear-thinning property at room temperature	363:439	Due to the formation of XG network composed of XG double helical strand structure, XG/MC blend was a high viscous solution with good shear-thinning property at room temperature.
26256374	2	56	theme	high	365:368	arg1	blend					353:357	XG/MC blend	347:357	XG/MC blend	347:357	Due to the formation of XG network composed of XG double helical strand structure, XG/MC blend was a high viscous solution with good shear-thinning property at room temperature.
26256374	0	57	theme	methylcellulose	66:80	arg1	networks					89:96	methylcellulose double networks	66:96	methylcellulose double networks with shear-thinning property	66:125	Injectable thermo-responsive hydrogel composed of xanthan gum and methylcellulose double networks with shear-thinning property.
26256374	4	58	theme	gelation	579:586	arg1	time					588:591	gelation time	579:591	gelation time	579:591	The gelation time and storage modulus of the blend can be tuned by XG and/or MC concentrations.
26256374	2	59	theme	XG/MC	347:351	arg1	solution					378:385	a high viscous solution	363:385	a high viscous solution with good shear-thinning property at room temperature	363:439	Due to the formation of XG network composed of XG double helical strand structure, XG/MC blend was a high viscous solution with good shear-thinning property at room temperature.
26256374	2	59	theme	XG/MC	347:351	arg1	blend					353:357	XG/MC blend	347:357	XG/MC blend	347:357	Due to the formation of XG network composed of XG double helical strand structure, XG/MC blend was a high viscous solution with good shear-thinning property at room temperature.
26256374	4	60	theme	MC	652:653	arg1	concentrations					655:668	MC concentrations	652:668	MC concentrations	652:668	The gelation time and storage modulus of the blend can be tuned by XG and/or MC concentrations.
24763427	1	0	theme	caries	158:163	arg1	factor					141:146	A major aetiological factor	120:146	A major aetiological factor of dental caries	120:163	A major aetiological factor of dental caries is the pathology of the dental plaque biofilms.
24763427	1	0	theme	caries	158:163	arg1	pathology					172:180	the pathology	168:180	the pathology of the dental plaque biofilms	168:210	A major aetiological factor of dental caries is the pathology of the dental plaque biofilms.
24763427	7	1	theme	additional	910:919	arg1	information					932:942	additional structural information	910:942	additional structural information on bacterial cells embedded within the surrounding extracellular matrix	910:1014	High-resolution imaging of biofilm surfaces can reveal additional structural information on bacterial cells embedded within the surrounding extracellular matrix.
24763427	4	2	theme	Arg	543:545	arg1	action					533:538	action	533:538	action of Arg	533:545	Here, we explored an alternative and complementary mechanism of action of Arg using atomic force microscopy.
24763427	0	3	from	characterization	10:25	arg1	adhesion					83:90	Streptococcus mutans biofilm adhesion	54:90	Streptococcus mutans biofilm adhesion	54:90	Nanoscale characterization of effect of L-arginine on Streptococcus mutans biofilm adhesion by atomic force microscopy.
24763427	5	4	theme	biofilm	632:638	arg1	matrix					654:659	Streptococcus mutans biofilm extracellular matrix	611:659	Streptococcus mutans biofilm extracellular matrix	611:659	The nanomechanical properties of Streptococcus mutans biofilm extracellular matrix were characterized under physiological buffer conditions.
24763427	8	5	theme	dense	1019:1023	arg1	matrix					1039:1044	A dense extracellular matrix	1017:1044	A dense extracellular matrix	1017:1044	A dense extracellular matrix was observed in biofilms without Arg compared to those grown in the presence of Arg.
24763427	6	6	from	effect	733:738	arg1	behaviour					763:771	adhesive behaviour	754:771	adhesive behaviour	754:771	We report the effect of Arg on the adhesive behaviour and structural properties of extracellular polysaccharides in S. mutans biofilms.
24763427	6	6	from	effect	733:738	arg1	properties					788:797	structural properties	777:797	structural properties	777:797	We report the effect of Arg on the adhesive behaviour and structural properties of extracellular polysaccharides in S. mutans biofilms.
24763427	7	7	theme	surfaces	890:897	arg1	imaging					871:877	High-resolution imaging	855:877	High-resolution imaging of biofilm surfaces	855:897	High-resolution imaging of biofilm surfaces can reveal additional structural information on bacterial cells embedded within the surrounding extracellular matrix.
24763427	8	8	located	observed	1050:1057	arg2	matrix					1039:1044	A dense extracellular matrix	1017:1044	A dense extracellular matrix	1017:1044	A dense extracellular matrix was observed in biofilms without Arg compared to those grown in the presence of Arg.
24763427	8	8	located	observed	1050:1057	arg1	biofilms					1062:1069	biofilms	1062:1069	biofilms without Arg	1062:1081	A dense extracellular matrix was observed in biofilms without Arg compared to those grown in the presence of Arg.
24763427	0	9	theme	force	102:106	arg1	microscopy					108:117	atomic force microscopy	95:117	atomic force microscopy	95:117	Nanoscale characterization of effect of L-arginine on Streptococcus mutans biofilm adhesion by atomic force microscopy.
24763427	9	10	theme	membrane	1246:1253	arg1	glucans					1255:1261	extracellular membrane glucans	1232:1261	extracellular membrane glucans	1232:1261	S. mutans biofilms grown in the presence of Arg could influence the production and/or composition of extracellular membrane glucans and thereby affect their adhesion properties.
24763427	5	11	theme	extracellular	640:652	arg1	matrix					654:659	Streptococcus mutans biofilm extracellular matrix	611:659	Streptococcus mutans biofilm extracellular matrix	611:659	The nanomechanical properties of Streptococcus mutans biofilm extracellular matrix were characterized under physiological buffer conditions.
24763427	6	12	theme	polysaccharides	816:830	arg1	behaviour					763:771	adhesive behaviour	754:771	adhesive behaviour	754:771	We report the effect of Arg on the adhesive behaviour and structural properties of extracellular polysaccharides in S. mutans biofilms.
24763427	6	12	theme	polysaccharides	816:830	arg1	properties					788:797	structural properties	777:797	structural properties	777:797	We report the effect of Arg on the adhesive behaviour and structural properties of extracellular polysaccharides in S. mutans biofilms.
24763427	4	13	theme	atomic	553:558	arg1	microscopy					566:575	atomic force microscopy	553:575	atomic force microscopy	553:575	Here, we explored an alternative and complementary mechanism of action of Arg using atomic force microscopy.
24763427	0	14	theme	atomic	95:100	arg1	microscopy					108:117	atomic force microscopy	95:117	atomic force microscopy	95:117	Nanoscale characterization of effect of L-arginine on Streptococcus mutans biofilm adhesion by atomic force microscopy.
24763427	10	15	from	presence	1338:1345	arg1	cavity					1366:1371	the oral cavity	1357:1371	the oral cavity	1357:1371	Our results suggest that the presence of Arg in the oral cavity could influence the adhesion properties of S. mutans to the tooth surface.
24763427	5	16	theme	matrix	654:659	arg1	properties					597:606	The nanomechanical properties	578:606	The nanomechanical properties of Streptococcus mutans biofilm extracellular matrix	578:659	The nanomechanical properties of Streptococcus mutans biofilm extracellular matrix were characterized under physiological buffer conditions.
24763427	6	17	theme	extracellular	802:814	arg1	polysaccharides					816:830	extracellular polysaccharides	802:830	extracellular polysaccharides	802:830	We report the effect of Arg on the adhesive behaviour and structural properties of extracellular polysaccharides in S. mutans biofilms.
24763427	2	18	theme	salivary	309:316	arg1	peptides					318:325	salivary peptides	309:325	salivary peptides	309:325	The amino acid L-arginine (Arg) is found naturally in saliva as a free molecule or as a part of salivary peptides and proteins.
24763427	3	19	theme	cariogenous	440:450	arg1	microbiome					457:466	a less cariogenous oral microbiome	433:466	a less cariogenous oral microbiome	433:466	Plaque bacteria metabolize Arg to produce alkali and neutralize glycolytic acids, promoting a less cariogenous oral microbiome.
24763427	10	20	attach	presence	1338:1345	arg2	Arg					1350:1352	Arg	1350:1352	Arg	1350:1352	Our results suggest that the presence of Arg in the oral cavity could influence the adhesion properties of S. mutans to the tooth surface.
24763427	10	20	attach	presence	1338:1345	arg1	cavity					1366:1371	the oral cavity	1357:1371	the oral cavity	1357:1371	Our results suggest that the presence of Arg in the oral cavity could influence the adhesion properties of S. mutans to the tooth surface.
24763427	5	21	theme	nanomechanical	582:595	arg1	properties					597:606	The nanomechanical properties	578:606	The nanomechanical properties of Streptococcus mutans biofilm extracellular matrix	578:659	The nanomechanical properties of Streptococcus mutans biofilm extracellular matrix were characterized under physiological buffer conditions.
24763427	6	22	from	behaviour	763:771	arg1	biofilms					845:852	S. mutans biofilms	835:852	S. mutans biofilms	835:852	We report the effect of Arg on the adhesive behaviour and structural properties of extracellular polysaccharides in S. mutans biofilms.
24763427	6	23	dep	behaviour	763:771	arg1	the					750:752	the	750:752	the	750:752	We report the effect of Arg on the adhesive behaviour and structural properties of extracellular polysaccharides in S. mutans biofilms.
24763427	9	24	theme	extracellular	1232:1244	arg1	glucans					1255:1261	extracellular membrane glucans	1232:1261	extracellular membrane glucans	1232:1261	S. mutans biofilms grown in the presence of Arg could influence the production and/or composition of extracellular membrane glucans and thereby affect their adhesion properties.
24763427	7	25	theme	High-resolution	855:869	arg1	imaging					871:877	High-resolution imaging	855:877	High-resolution imaging of biofilm surfaces	855:897	High-resolution imaging of biofilm surfaces can reveal additional structural information on bacterial cells embedded within the surrounding extracellular matrix.
24763427	3	26	theme	glycolytic	405:414	arg1	acids					416:420	glycolytic acids	405:420	glycolytic acids	405:420	Plaque bacteria metabolize Arg to produce alkali and neutralize glycolytic acids, promoting a less cariogenous oral microbiome.
24763427	3	27	theme	Plaque	341:346	arg1	bacteria					348:355	Plaque bacteria	341:355	Plaque bacteria	341:355	Plaque bacteria metabolize Arg to produce alkali and neutralize glycolytic acids, promoting a less cariogenous oral microbiome.
24763427	6	28	theme	structural	777:786	arg1	properties					788:797	structural properties	777:797	structural properties	777:797	We report the effect of Arg on the adhesive behaviour and structural properties of extracellular polysaccharides in S. mutans biofilms.
24763427	7	29	theme	surrounding	983:993	arg1	matrix					1009:1014	the surrounding extracellular matrix	979:1014	the surrounding extracellular matrix	979:1014	High-resolution imaging of biofilm surfaces can reveal additional structural information on bacterial cells embedded within the surrounding extracellular matrix.
24763427	0	30	theme	Nanoscale	0:8	arg1	characterization					10:25	Nanoscale characterization	0:25	Nanoscale characterization of effect of L-arginine on Streptococcus mutans biofilm adhesion by atomic force microscopy.	0:118	Nanoscale characterization of effect of L-arginine on Streptococcus mutans biofilm adhesion by atomic force microscopy.
24763427	10	31	theme	tooth	1433:1437	arg1	surface					1439:1445	the tooth surface	1429:1445	the tooth surface	1429:1445	Our results suggest that the presence of Arg in the oral cavity could influence the adhesion properties of S. mutans to the tooth surface.
24763427	5	32	theme	Streptococcus	611:623	arg1	matrix					654:659	Streptococcus mutans biofilm extracellular matrix	611:659	Streptococcus mutans biofilm extracellular matrix	611:659	The nanomechanical properties of Streptococcus mutans biofilm extracellular matrix were characterized under physiological buffer conditions.
24763427	9	33	theme	glucans	1255:1261	arg1	production					1199:1208	production	1199:1208	production	1199:1208	S. mutans biofilms grown in the presence of Arg could influence the production and/or composition of extracellular membrane glucans and thereby affect their adhesion properties.
24763427	2	34	theme	free	279:282	arg1	L-arginine					228:237	The amino acid L-arginine	213:237	The amino acid L-arginine (Arg)	213:243	The amino acid L-arginine (Arg) is found naturally in saliva as a free molecule or as a part of salivary peptides and proteins.
24763427	2	34	theme	free	279:282	arg1	molecule					284:291	a free molecule	277:291	a free molecule	277:291	The amino acid L-arginine (Arg) is found naturally in saliva as a free molecule or as a part of salivary peptides and proteins.
24763427	9	35	theme	S.	1131:1132	arg1	biofilms					1141:1148	S. mutans biofilms	1131:1148	S. mutans biofilms grown in the presence of Arg	1131:1177	S. mutans biofilms grown in the presence of Arg could influence the production and/or composition of extracellular membrane glucans and thereby affect their adhesion properties.
24763427	2	36	theme	proteins	331:338	arg1	part					301:304	a part	299:304	a part of salivary peptides and proteins	299:338	The amino acid L-arginine (Arg) is found naturally in saliva as a free molecule or as a part of salivary peptides and proteins.
24763427	2	36	theme	proteins	331:338	arg1	L-arginine					228:237	The amino acid L-arginine	213:237	The amino acid L-arginine (Arg)	213:243	The amino acid L-arginine (Arg) is found naturally in saliva as a free molecule or as a part of salivary peptides and proteins.
24763427	0	37	theme	effect	30:35	arg1	characterization					10:25	Nanoscale characterization	0:25	Nanoscale characterization of effect of L-arginine on Streptococcus mutans biofilm adhesion by atomic force microscopy.	0:118	Nanoscale characterization of effect of L-arginine on Streptococcus mutans biofilm adhesion by atomic force microscopy.
24763427	4	38	theme	complementary	506:518	arg1	mechanism					520:528	an alternative and complementary mechanism	487:528	an alternative and complementary mechanism of action of Arg	487:545	Here, we explored an alternative and complementary mechanism of action of Arg using atomic force microscopy.
24763427	5	39	theme	buffer	700:705	arg1	conditions					707:716	physiological buffer conditions	686:716	physiological buffer conditions	686:716	The nanomechanical properties of Streptococcus mutans biofilm extracellular matrix were characterized under physiological buffer conditions.
24763427	7	40	theme	embedded	963:970	arg1	cells					957:961	bacterial cells	947:961	bacterial cells embedded within the surrounding extracellular matrix	947:1014	High-resolution imaging of biofilm surfaces can reveal additional structural information on bacterial cells embedded within the surrounding extracellular matrix.
24763427	10	41	theme	Arg	1350:1352	arg1	presence					1338:1345	the presence	1334:1345	the presence of Arg in the oral cavity	1334:1371	Our results suggest that the presence of Arg in the oral cavity could influence the adhesion properties of S. mutans to the tooth surface.
24763427	9	42	theme	mutans	1134:1139	arg1	biofilms					1141:1148	S. mutans biofilms	1131:1148	S. mutans biofilms grown in the presence of Arg	1131:1177	S. mutans biofilms grown in the presence of Arg could influence the production and/or composition of extracellular membrane glucans and thereby affect their adhesion properties.
24763427	1	43	theme	dental	189:194	arg1	biofilms					203:210	the dental plaque biofilms	185:210	the dental plaque biofilms	185:210	A major aetiological factor of dental caries is the pathology of the dental plaque biofilms.
24763427	7	44	from	information	932:942	arg1	cells					957:961	bacterial cells	947:961	bacterial cells embedded within the surrounding extracellular matrix	947:1014	High-resolution imaging of biofilm surfaces can reveal additional structural information on bacterial cells embedded within the surrounding extracellular matrix.
24763427	4	45	theme	action	533:538	arg1	mechanism					520:528	an alternative and complementary mechanism	487:528	an alternative and complementary mechanism of action of Arg	487:545	Here, we explored an alternative and complementary mechanism of action of Arg using atomic force microscopy.
24763427	7	46	theme	extracellular	995:1007	arg1	matrix					1009:1014	the surrounding extracellular matrix	979:1014	the surrounding extracellular matrix	979:1014	High-resolution imaging of biofilm surfaces can reveal additional structural information on bacterial cells embedded within the surrounding extracellular matrix.
24763427	1	47	theme	plaque	196:201	arg1	biofilms					203:210	the dental plaque biofilms	185:210	the dental plaque biofilms	185:210	A major aetiological factor of dental caries is the pathology of the dental plaque biofilms.
24763427	0	48	theme	L-arginine	40:49	arg1	effect					30:35	effect	30:35	effect of L-arginine on Streptococcus mutans biofilm adhesion	30:90	Nanoscale characterization of effect of L-arginine on Streptococcus mutans biofilm adhesion by atomic force microscopy.
24763427	7	49	theme	biofilm	882:888	arg1	surfaces					890:897	biofilm surfaces	882:897	biofilm surfaces	882:897	High-resolution imaging of biofilm surfaces can reveal additional structural information on bacterial cells embedded within the surrounding extracellular matrix.
24763427	0	50	from	adhesion	83:90	arg1	characterization					10:25	Nanoscale characterization	0:25	Nanoscale characterization of effect of L-arginine on Streptococcus mutans biofilm adhesion by atomic force microscopy.	0:118	Nanoscale characterization of effect of L-arginine on Streptococcus mutans biofilm adhesion by atomic force microscopy.
24763427	1	51	theme	biofilms	203:210	arg1	factor					141:146	A major aetiological factor	120:146	A major aetiological factor of dental caries	120:163	A major aetiological factor of dental caries is the pathology of the dental plaque biofilms.
24763427	1	51	theme	biofilms	203:210	arg1	pathology					172:180	the pathology	168:180	the pathology of the dental plaque biofilms	168:210	A major aetiological factor of dental caries is the pathology of the dental plaque biofilms.
24763427	5	52	dep	Streptococcus	611:623	arg1	mutans					625:630	mutans	625:630	mutans	625:630	The nanomechanical properties of Streptococcus mutans biofilm extracellular matrix were characterized under physiological buffer conditions.
24763427	8	53	theme	Arg	1126:1128	arg1	presence					1114:1121	the presence	1110:1121	the presence of Arg	1110:1128	A dense extracellular matrix was observed in biofilms without Arg compared to those grown in the presence of Arg.
24763427	2	54	theme	acid	223:226	arg1	Arg					240:242	Arg	240:242	Arg	240:242	The amino acid L-arginine (Arg) is found naturally in saliva as a free molecule or as a part of salivary peptides and proteins.
24763427	2	54	theme	acid	223:226	arg1	L-arginine					228:237	The amino acid L-arginine	213:237	The amino acid L-arginine (Arg)	213:243	The amino acid L-arginine (Arg) is found naturally in saliva as a free molecule or as a part of salivary peptides and proteins.
24763427	2	54	theme	acid	223:226	arg1	part					301:304	a part	299:304	a part of salivary peptides and proteins	299:338	The amino acid L-arginine (Arg) is found naturally in saliva as a free molecule or as a part of salivary peptides and proteins.
24763427	2	54	theme	acid	223:226	arg1	molecule					284:291	a free molecule	277:291	a free molecule	277:291	The amino acid L-arginine (Arg) is found naturally in saliva as a free molecule or as a part of salivary peptides and proteins.
24763427	6	55	theme	adhesive	754:761	arg1	behaviour					763:771	adhesive behaviour	754:771	adhesive behaviour	754:771	We report the effect of Arg on the adhesive behaviour and structural properties of extracellular polysaccharides in S. mutans biofilms.
24763427	0	56	theme	Streptococcus	54:66	arg1	adhesion					83:90	Streptococcus mutans biofilm adhesion	54:90	Streptococcus mutans biofilm adhesion	54:90	Nanoscale characterization of effect of L-arginine on Streptococcus mutans biofilm adhesion by atomic force microscopy.
24763427	9	57	dep	influence	1185:1193	arg1	affect					1275:1280	affect	1275:1280	affect their adhesion properties	1275:1306	S. mutans biofilms grown in the presence of Arg could influence the production and/or composition of extracellular membrane glucans and thereby affect their adhesion properties.
24763427	9	58	theme	adhesion	1288:1295	arg1	properties					1297:1306	their adhesion properties	1282:1306	their adhesion properties	1282:1306	S. mutans biofilms grown in the presence of Arg could influence the production and/or composition of extracellular membrane glucans and thereby affect their adhesion properties.
24763427	2	59	theme	amino	217:221	arg1	Arg					240:242	Arg	240:242	Arg	240:242	The amino acid L-arginine (Arg) is found naturally in saliva as a free molecule or as a part of salivary peptides and proteins.
24763427	2	59	theme	amino	217:221	arg1	L-arginine					228:237	The amino acid L-arginine	213:237	The amino acid L-arginine (Arg)	213:243	The amino acid L-arginine (Arg) is found naturally in saliva as a free molecule or as a part of salivary peptides and proteins.
24763427	2	59	theme	amino	217:221	arg1	part					301:304	a part	299:304	a part of salivary peptides and proteins	299:338	The amino acid L-arginine (Arg) is found naturally in saliva as a free molecule or as a part of salivary peptides and proteins.
24763427	2	59	theme	amino	217:221	arg1	molecule					284:291	a free molecule	277:291	a free molecule	277:291	The amino acid L-arginine (Arg) is found naturally in saliva as a free molecule or as a part of salivary peptides and proteins.
24763427	3	60	theme	oral	452:455	arg1	microbiome					457:466	a less cariogenous oral microbiome	433:466	a less cariogenous oral microbiome	433:466	Plaque bacteria metabolize Arg to produce alkali and neutralize glycolytic acids, promoting a less cariogenous oral microbiome.
24763427	10	61	theme	oral	1361:1364	arg1	cavity					1366:1371	the oral cavity	1357:1371	the oral cavity	1357:1371	Our results suggest that the presence of Arg in the oral cavity could influence the adhesion properties of S. mutans to the tooth surface.
24763427	2	62	located	found	248:252	arg2	molecule					284:291	a free molecule	277:291	a free molecule	277:291	The amino acid L-arginine (Arg) is found naturally in saliva as a free molecule or as a part of salivary peptides and proteins.
24763427	2	62	located	found	248:252	arg2	part					301:304	a part	299:304	a part of salivary peptides and proteins	299:338	The amino acid L-arginine (Arg) is found naturally in saliva as a free molecule or as a part of salivary peptides and proteins.
24763427	2	62	located	found	248:252	arg2	Arg					240:242	Arg	240:242	Arg	240:242	The amino acid L-arginine (Arg) is found naturally in saliva as a free molecule or as a part of salivary peptides and proteins.
24763427	2	62	located	found	248:252	arg1	saliva					267:272	saliva	267:272	saliva	267:272	The amino acid L-arginine (Arg) is found naturally in saliva as a free molecule or as a part of salivary peptides and proteins.
24763427	2	62	located	found	248:252	arg2	L-arginine					228:237	The amino acid L-arginine	213:237	The amino acid L-arginine (Arg)	213:243	The amino acid L-arginine (Arg) is found naturally in saliva as a free molecule or as a part of salivary peptides and proteins.
24763427	10	63	theme	adhesion	1393:1400	arg1	properties					1402:1411	the adhesion properties	1389:1411	the adhesion properties of S. mutans to the tooth surface	1389:1445	Our results suggest that the presence of Arg in the oral cavity could influence the adhesion properties of S. mutans to the tooth surface.
24763427	1	64	theme	major	122:126	arg1	factor					141:146	A major aetiological factor	120:146	A major aetiological factor of dental caries	120:163	A major aetiological factor of dental caries is the pathology of the dental plaque biofilms.
24763427	1	64	theme	major	122:126	arg1	pathology					172:180	the pathology	168:180	the pathology of the dental plaque biofilms	168:210	A major aetiological factor of dental caries is the pathology of the dental plaque biofilms.
24763427	4	65	theme	alternative	490:500	arg1	mechanism					520:528	an alternative and complementary mechanism	487:528	an alternative and complementary mechanism of action of Arg	487:545	Here, we explored an alternative and complementary mechanism of action of Arg using atomic force microscopy.
24763427	6	66	theme	Arg	743:745	arg1	effect					733:738	the effect	729:738	the effect of Arg on the adhesive behaviour and structural properties of extracellular polysaccharides in S. mutans biofilms	729:852	We report the effect of Arg on the adhesive behaviour and structural properties of extracellular polysaccharides in S. mutans biofilms.
24763427	1	67	theme	aetiological	128:139	arg1	factor					141:146	A major aetiological factor	120:146	A major aetiological factor of dental caries	120:163	A major aetiological factor of dental caries is the pathology of the dental plaque biofilms.
24763427	1	67	theme	aetiological	128:139	arg1	pathology					172:180	the pathology	168:180	the pathology of the dental plaque biofilms	168:210	A major aetiological factor of dental caries is the pathology of the dental plaque biofilms.
24763427	7	68	theme	bacterial	947:955	arg1	cells					957:961	bacterial cells	947:961	bacterial cells embedded within the surrounding extracellular matrix	947:1014	High-resolution imaging of biofilm surfaces can reveal additional structural information on bacterial cells embedded within the surrounding extracellular matrix.
24763427	9	69	dep	production	1199:1208	arg1	the					1195:1197	the	1195:1197	the	1195:1197	S. mutans biofilms grown in the presence of Arg could influence the production and/or composition of extracellular membrane glucans and thereby affect their adhesion properties.
24763427	5	70	theme	physiological	686:698	arg1	conditions					707:716	physiological buffer conditions	686:716	physiological buffer conditions	686:716	The nanomechanical properties of Streptococcus mutans biofilm extracellular matrix were characterized under physiological buffer conditions.
24763427	6	71	theme	mutans	838:843	arg1	biofilms					845:852	S. mutans biofilms	835:852	S. mutans biofilms	835:852	We report the effect of Arg on the adhesive behaviour and structural properties of extracellular polysaccharides in S. mutans biofilms.
24763427	9	72	theme	Arg	1175:1177	arg1	presence					1163:1170	the presence	1159:1170	the presence of Arg	1159:1177	S. mutans biofilms grown in the presence of Arg could influence the production and/or composition of extracellular membrane glucans and thereby affect their adhesion properties.
24763427	1	73	theme	dental	151:156	arg1	caries					158:163	dental caries	151:163	dental caries	151:163	A major aetiological factor of dental caries is the pathology of the dental plaque biofilms.
24763427	7	74	theme	structural	921:930	arg1	information					932:942	additional structural information	910:942	additional structural information on bacterial cells embedded within the surrounding extracellular matrix	910:1014	High-resolution imaging of biofilm surfaces can reveal additional structural information on bacterial cells embedded within the surrounding extracellular matrix.
24763427	4	75	theme	force	560:564	arg1	microscopy					566:575	atomic force microscopy	553:575	atomic force microscopy	553:575	Here, we explored an alternative and complementary mechanism of action of Arg using atomic force microscopy.
24763427	0	76	theme	biofilm	75:81	arg1	adhesion					83:90	Streptococcus mutans biofilm adhesion	54:90	Streptococcus mutans biofilm adhesion	54:90	Nanoscale characterization of effect of L-arginine on Streptococcus mutans biofilm adhesion by atomic force microscopy.
24763427	10	77	theme	mutans	1419:1424	arg1	properties					1402:1411	the adhesion properties	1389:1411	the adhesion properties of S. mutans to the tooth surface	1389:1445	Our results suggest that the presence of Arg in the oral cavity could influence the adhesion properties of S. mutans to the tooth surface.
24763427	8	78	theme	extracellular	1025:1037	arg1	matrix					1039:1044	A dense extracellular matrix	1017:1044	A dense extracellular matrix	1017:1044	A dense extracellular matrix was observed in biofilms without Arg compared to those grown in the presence of Arg.
24763427	0	79	theme	mutans	68:73	arg1	adhesion					83:90	Streptococcus mutans biofilm adhesion	54:90	Streptococcus mutans biofilm adhesion	54:90	Nanoscale characterization of effect of L-arginine on Streptococcus mutans biofilm adhesion by atomic force microscopy.
24763427	0	80	from	effect	30:35	arg1	adhesion					83:90	Streptococcus mutans biofilm adhesion	54:90	Streptococcus mutans biofilm adhesion	54:90	Nanoscale characterization of effect of L-arginine on Streptococcus mutans biofilm adhesion by atomic force microscopy.
24763427	6	81	from	properties	788:797	arg1	biofilms					845:852	S. mutans biofilms	835:852	S. mutans biofilms	835:852	We report the effect of Arg on the adhesive behaviour and structural properties of extracellular polysaccharides in S. mutans biofilms.
24763427	2	82	theme	peptides	318:325	arg1	part					301:304	a part	299:304	a part of salivary peptides and proteins	299:338	The amino acid L-arginine (Arg) is found naturally in saliva as a free molecule or as a part of salivary peptides and proteins.
24763427	2	82	theme	peptides	318:325	arg1	L-arginine					228:237	The amino acid L-arginine	213:237	The amino acid L-arginine (Arg)	213:243	The amino acid L-arginine (Arg) is found naturally in saliva as a free molecule or as a part of salivary peptides and proteins.
26794733	8	0	theme	copper	1035:1040	arg1	ions					1042:1045	copper ions	1035:1045	copper ions	1035:1045	Characteristics and physical properties of the prepared CT/HNT beads were also analyzed by SEM, FTIR, TGA and BET the results show that introducing HNT to chitosan increases the adsorption capacity toward copper ions; however HNT loading fraction above 50wt.
26794733	10	1	theme	beads	1231:1235	arg1	capacity					1208:1215	The adsorption capacity	1193:1215	The adsorption capacity of the CT/HNT beads prepared from Dragonite source	1193:1266	The adsorption capacity of the CT/HNT beads prepared from Dragonite source had a larger adsorption capacity of 14.2mg/g as compared to that of Matauri Bay, 10.55mg/g.
26794733	11	2	theme	adsorption	1385:1394	arg1	capacity					1396:1403	the adsorption capacity	1381:1403	the adsorption capacity of the beads toward copper ions	1381:1435	It was observed that the adsorption capacity of the beads toward copper ions decreased when the loading fraction of HNT is increased at constant ultrasound amplitude.
26794733	8	3	theme	CT/HNT	886:891	arg1	beads					893:897	the prepared CT/HNT beads	873:897	the prepared CT/HNT beads	873:897	Characteristics and physical properties of the prepared CT/HNT beads were also analyzed by SEM, FTIR, TGA and BET the results show that introducing HNT to chitosan increases the adsorption capacity toward copper ions; however HNT loading fraction above 50wt.
26794733	7	4	from	frequency	799:807	arg1	amplitude					777:785	the amplitude	773:785	the amplitude at constant frequency of 25%, 50% and 75%	773:827	The effect of ultrasound as a mixing device was also studied by varying the amplitude at constant frequency of 25%, 50% and 75%.
26794733	0	5	theme	ion	119:121	arg1	removal					123:129	copper ion removal	112:129	copper ion removal	112:129	Chitosan/halloysite beads fabricated by ultrasonic-assisted extrusion-dripping and a case study application for copper ion removal.
26794733	9	6	theme	amino	1179:1183	arg1	groups					1185:1190	the amino groups	1175:1190	the amino groups	1175:1190	% resulted in a decrease in adsorption capacity attributed to limited accessibility of the amino groups.
26794733	8	7	theme	prepared	877:884	arg1	beads					893:897	the prepared CT/HNT beads	873:897	the prepared CT/HNT beads	873:897	Characteristics and physical properties of the prepared CT/HNT beads were also analyzed by SEM, FTIR, TGA and BET the results show that introducing HNT to chitosan increases the adsorption capacity toward copper ions; however HNT loading fraction above 50wt.
26794733	11	8	theme	HNT	1476:1478	arg1	fraction					1464:1471	the loading fraction	1452:1471	the loading fraction of HNT	1452:1478	It was observed that the adsorption capacity of the beads toward copper ions decreased when the loading fraction of HNT is increased at constant ultrasound amplitude.
26794733	0	9	theme	copper	112:117	arg1	removal					123:129	copper ion removal	112:129	copper ion removal	112:129	Chitosan/halloysite beads fabricated by ultrasonic-assisted extrusion-dripping and a case study application for copper ion removal.
26794733	9	10	theme	groups	1185:1190	arg1	accessibility					1158:1170	limited accessibility	1150:1170	limited accessibility of the amino groups	1150:1190	% resulted in a decrease in adsorption capacity attributed to limited accessibility of the amino groups.
26794733	12	11	theme	beads	1645:1649	arg1	characteristics					1594:1608	characteristics	1594:1608	characteristics	1594:1608	The result of this study helps to understand the links between the characteristics and adsorption abilities of CT/HNT beads.
26794733	12	11	theme	beads	1645:1649	arg1	abilities					1625:1633	adsorption abilities	1614:1633	adsorption abilities	1614:1633	The result of this study helps to understand the links between the characteristics and adsorption abilities of CT/HNT beads.
26794733	7	12	theme	constant	790:797	arg1	frequency					799:807	constant frequency	790:807	constant frequency of 25%, 50% and 75%	790:827	The effect of ultrasound as a mixing device was also studied by varying the amplitude at constant frequency of 25%, 50% and 75%.
26794733	8	13	theme	HNT	1056:1058	arg1	loading					1060:1066	HNT loading	1056:1066	HNT loading	1056:1066	Characteristics and physical properties of the prepared CT/HNT beads were also analyzed by SEM, FTIR, TGA and BET the results show that introducing HNT to chitosan increases the adsorption capacity toward copper ions; however HNT loading fraction above 50wt.
26794733	8	14	dep	SEM	921:923	arg1	show					956:959	show	956:959	show that introducing HNT to chitosan increases the adsorption capacity toward copper ions; however HNT loading fraction above 50wt	956:1086	Characteristics and physical properties of the prepared CT/HNT beads were also analyzed by SEM, FTIR, TGA and BET the results show that introducing HNT to chitosan increases the adsorption capacity toward copper ions; however HNT loading fraction above 50wt.
26794733	8	15	theme	beads	893:897	arg1	Characteristics					830:844	Characteristics	830:844	Characteristics	830:844	Characteristics and physical properties of the prepared CT/HNT beads were also analyzed by SEM, FTIR, TGA and BET the results show that introducing HNT to chitosan increases the adsorption capacity toward copper ions; however HNT loading fraction above 50wt.
26794733	8	15	theme	beads	893:897	arg1	properties					859:868	physical properties	850:868	physical properties	850:868	Characteristics and physical properties of the prepared CT/HNT beads were also analyzed by SEM, FTIR, TGA and BET the results show that introducing HNT to chitosan increases the adsorption capacity toward copper ions; however HNT loading fraction above 50wt.
26794733	2	16	theme	potential	401:409	arg1	intake					389:394	the copper removal intake	370:394	the copper removal intake	370:394	This study concentrates on development of a biopolymer based on chitosan (CT) and halloysite nanotubes (HNT) and evaluates the copper removal intake as a potential application of this bio-composite.
26794733	2	16	theme	potential	401:409	arg1	application					411:421	a potential application	399:421	a potential application of this bio-composite	399:443	This study concentrates on development of a biopolymer based on chitosan (CT) and halloysite nanotubes (HNT) and evaluates the copper removal intake as a potential application of this bio-composite.
26794733	9	17	from	decrease	1104:1111	arg1	capacity					1127:1134	adsorption capacity	1116:1134	adsorption capacity attributed to limited accessibility of the amino groups	1116:1190	% resulted in a decrease in adsorption capacity attributed to limited accessibility of the amino groups.
26794733	4	18	dep	HNTs	572:575	arg1	HNTs					572:575	HNTs	572:575	HNTs (i.e. Dragonite and Matauri Bay)	572:608	Two sources of HNTs (i.e. Dragonite and Matauri Bay) were added into a chitosan solution (2wt.
26794733	4	18	dep	HNTs	572:575	arg1	Dragonite					583:591	Dragonite	583:591	Dragonite	583:591	Two sources of HNTs (i.e. Dragonite and Matauri Bay) were added into a chitosan solution (2wt.
26794733	4	18	dep	HNTs	572:575	arg1	Bay					605:607	Bay	605:607	Bay	605:607	Two sources of HNTs (i.e. Dragonite and Matauri Bay) were added into a chitosan solution (2wt.
26794733	10	19	theme	14.2mg/g	1304:1311	arg1	capacity					1292:1299	a larger adsorption capacity	1272:1299	a larger adsorption capacity of 14.2mg/g	1272:1311	The adsorption capacity of the CT/HNT beads prepared from Dragonite source had a larger adsorption capacity of 14.2mg/g as compared to that of Matauri Bay, 10.55mg/g.
26794733	12	20	theme	CT/HNT	1638:1643	arg1	beads					1645:1649	CT/HNT beads	1638:1649	CT/HNT beads	1638:1649	The result of this study helps to understand the links between the characteristics and adsorption abilities of CT/HNT beads.
26794733	0	21	theme	Chitosan/halloysite	0:18	arg1	beads					20:24	Chitosan/halloysite beads	0:24	Chitosan/halloysite beads	0:24	Chitosan/halloysite beads fabricated by ultrasonic-assisted extrusion-dripping and a case study application for copper ion removal.
26794733	4	22	theme	HNTs	572:575	arg1	sources					561:567	Two sources	557:567	Two sources of HNTs (i.e. Dragonite and Matauri Bay)	557:608	Two sources of HNTs (i.e. Dragonite and Matauri Bay) were added into a chitosan solution (2wt.
26794733	7	23	theme	%	827:827	arg1	frequency					799:807	constant frequency	790:807	constant frequency of 25%, 50% and 75%	790:827	The effect of ultrasound as a mixing device was also studied by varying the amplitude at constant frequency of 25%, 50% and 75%.
26794733	2	24	theme	halloysite	329:338	arg1	HNT					351:353	HNT	351:353	HNT	351:353	This study concentrates on development of a biopolymer based on chitosan (CT) and halloysite nanotubes (HNT) and evaluates the copper removal intake as a potential application of this bio-composite.
26794733	2	24	theme	halloysite	329:338	arg1	nanotubes					340:348	halloysite nanotubes	329:348	halloysite nanotubes (HNT)	329:354	This study concentrates on development of a biopolymer based on chitosan (CT) and halloysite nanotubes (HNT) and evaluates the copper removal intake as a potential application of this bio-composite.
26794733	10	25	theme	adsorption	1197:1206	arg1	capacity					1208:1215	The adsorption capacity	1193:1215	The adsorption capacity of the CT/HNT beads prepared from Dragonite source	1193:1266	The adsorption capacity of the CT/HNT beads prepared from Dragonite source had a larger adsorption capacity of 14.2mg/g as compared to that of Matauri Bay, 10.55mg/g.
26794733	12	26	theme	adsorption	1614:1623	arg1	abilities					1625:1633	adsorption abilities	1614:1633	adsorption abilities	1614:1633	The result of this study helps to understand the links between the characteristics and adsorption abilities of CT/HNT beads.
26794733	10	27	theme	adsorption	1281:1290	arg1	capacity					1292:1299	a larger adsorption capacity	1272:1299	a larger adsorption capacity of 14.2mg/g	1272:1311	The adsorption capacity of the CT/HNT beads prepared from Dragonite source had a larger adsorption capacity of 14.2mg/g as compared to that of Matauri Bay, 10.55mg/g.
26794733	10	28	theme	larger	1274:1279	arg1	capacity					1292:1299	a larger adsorption capacity	1272:1299	a larger adsorption capacity of 14.2mg/g	1272:1311	The adsorption capacity of the CT/HNT beads prepared from Dragonite source had a larger adsorption capacity of 14.2mg/g as compared to that of Matauri Bay, 10.55mg/g.
26794733	2	29	theme	bio-composite	431:443	arg1	intake					389:394	the copper removal intake	370:394	the copper removal intake	370:394	This study concentrates on development of a biopolymer based on chitosan (CT) and halloysite nanotubes (HNT) and evaluates the copper removal intake as a potential application of this bio-composite.
26794733	2	29	theme	bio-composite	431:443	arg1	application					411:421	a potential application	399:421	a potential application of this bio-composite	399:443	This study concentrates on development of a biopolymer based on chitosan (CT) and halloysite nanotubes (HNT) and evaluates the copper removal intake as a potential application of this bio-composite.
26794733	8	30	theme	physical	850:857	arg1	properties					859:868	physical properties	850:868	physical properties	850:868	Characteristics and physical properties of the prepared CT/HNT beads were also analyzed by SEM, FTIR, TGA and BET the results show that introducing HNT to chitosan increases the adsorption capacity toward copper ions; however HNT loading fraction above 50wt.
26794733	7	31	theme	mixing	731:736	arg1	device					738:743	a mixing device	729:743	a mixing device	729:743	The effect of ultrasound as a mixing device was also studied by varying the amplitude at constant frequency of 25%, 50% and 75%.
26794733	9	32	theme	adsorption	1116:1125	arg1	capacity					1127:1134	adsorption capacity	1116:1134	adsorption capacity attributed to limited accessibility of the amino groups	1116:1190	% resulted in a decrease in adsorption capacity attributed to limited accessibility of the amino groups.
26794733	0	33	theme	extrusion-dripping	60:77	arg1	application					96:106	ultrasonic-assisted extrusion-dripping and a case study application	40:106	ultrasonic-assisted extrusion-dripping and a case study application for copper ion removal	40:129	Chitosan/halloysite beads fabricated by ultrasonic-assisted extrusion-dripping and a case study application for copper ion removal.
26794733	12	34	theme	study	1546:1550	arg1	result					1531:1536	The result	1527:1536	The result of this study	1527:1550	The result of this study helps to understand the links between the characteristics and adsorption abilities of CT/HNT beads.
26794733	0	35	theme	ultrasonic-assisted	40:58	arg1	application					96:106	ultrasonic-assisted extrusion-dripping and a case study application	40:106	ultrasonic-assisted extrusion-dripping and a case study application for copper ion removal	40:129	Chitosan/halloysite beads fabricated by ultrasonic-assisted extrusion-dripping and a case study application for copper ion removal.
26794733	8	36	theme	introducing	966:976	arg1	HNT					978:980	introducing HNT	966:980	introducing HNT to chitosan	966:992	Characteristics and physical properties of the prepared CT/HNT beads were also analyzed by SEM, FTIR, TGA and BET the results show that introducing HNT to chitosan increases the adsorption capacity toward copper ions; however HNT loading fraction above 50wt.
26794733	11	37	theme	constant	1496:1503	arg1	amplitude					1516:1524	constant ultrasound amplitude	1496:1524	constant ultrasound amplitude	1496:1524	It was observed that the adsorption capacity of the beads toward copper ions decreased when the loading fraction of HNT is increased at constant ultrasound amplitude.
26794733	4	38	dep	solution	637:644	arg1	2wt					647:649	2wt	647:649	2wt	647:649	Two sources of HNTs (i.e. Dragonite and Matauri Bay) were added into a chitosan solution (2wt.
26794733	10	39	theme	CT/HNT	1224:1229	arg1	beads					1231:1235	the CT/HNT beads	1220:1235	the CT/HNT beads prepared from Dragonite source	1220:1266	The adsorption capacity of the CT/HNT beads prepared from Dragonite source had a larger adsorption capacity of 14.2mg/g as compared to that of Matauri Bay, 10.55mg/g.
26794733	3	40	theme	CT/HNT	461:466	arg1	beads					468:472	CT/HNT beads	461:472	CT/HNT beads	461:472	In this study, CT/HNT beads were prepared by ultrasonic-assisted extrusion-dripping method for the first time.
26794733	8	41	theme	adsorption	1008:1017	arg1	capacity					1019:1026	the adsorption capacity	1004:1026	the adsorption capacity toward copper ions	1004:1045	Characteristics and physical properties of the prepared CT/HNT beads were also analyzed by SEM, FTIR, TGA and BET the results show that introducing HNT to chitosan increases the adsorption capacity toward copper ions; however HNT loading fraction above 50wt.
26794733	3	42	theme	ultrasonic-assisted	491:509	arg1	method					530:535	ultrasonic-assisted extrusion-dripping method	491:535	ultrasonic-assisted extrusion-dripping method for the first time	491:554	In this study, CT/HNT beads were prepared by ultrasonic-assisted extrusion-dripping method for the first time.
26794733	2	43	theme	copper	374:379	arg1	intake					389:394	the copper removal intake	370:394	the copper removal intake	370:394	This study concentrates on development of a biopolymer based on chitosan (CT) and halloysite nanotubes (HNT) and evaluates the copper removal intake as a potential application of this bio-composite.
26794733	2	43	theme	copper	374:379	arg1	application					411:421	a potential application	399:421	a potential application of this bio-composite	399:443	This study concentrates on development of a biopolymer based on chitosan (CT) and halloysite nanotubes (HNT) and evaluates the copper removal intake as a potential application of this bio-composite.
26794733	5	44	theme	various	657:663	arg1	fractions					673:681	various loading fractions	657:681	various loading fractions (25, 50	657:689	%) at various loading fractions (25, 50, 75wt.
26794733	1	45	theme	different	165:173	arg1	applications					175:186	different applications	165:186	different applications especially as bio-composites	165:215	Development of new materials for different applications especially as bio-composites has received great attention.
26794733	11	46	theme	loading	1456:1462	arg1	fraction					1464:1471	the loading fraction	1452:1471	the loading fraction of HNT	1452:1478	It was observed that the adsorption capacity of the beads toward copper ions decreased when the loading fraction of HNT is increased at constant ultrasound amplitude.
26794733	3	47	theme	extrusion-dripping	511:528	arg1	method					530:535	ultrasonic-assisted extrusion-dripping method	491:535	ultrasonic-assisted extrusion-dripping method for the first time	491:554	In this study, CT/HNT beads were prepared by ultrasonic-assisted extrusion-dripping method for the first time.
26794733	7	48	theme	ultrasound	715:724	arg1	effect					705:710	The effect	701:710	The effect of ultrasound as a mixing device	701:743	The effect of ultrasound as a mixing device was also studied by varying the amplitude at constant frequency of 25%, 50% and 75%.
26794733	5	49	theme	loading	665:671	arg1	fractions					673:681	various loading fractions	657:681	various loading fractions (25, 50	657:689	%) at various loading fractions (25, 50, 75wt.
26794733	11	50	theme	beads	1412:1416	arg1	capacity					1396:1403	the adsorption capacity	1381:1403	the adsorption capacity of the beads toward copper ions	1381:1435	It was observed that the adsorption capacity of the beads toward copper ions decreased when the loading fraction of HNT is increased at constant ultrasound amplitude.
26794733	1	51	theme	new	147:149	arg1	materials					151:159	new materials	147:159	new materials	147:159	Development of new materials for different applications especially as bio-composites has received great attention.
26794733	11	52	theme	copper	1425:1430	arg1	ions					1432:1435	copper ions	1425:1435	copper ions	1425:1435	It was observed that the adsorption capacity of the beads toward copper ions decreased when the loading fraction of HNT is increased at constant ultrasound amplitude.
26794733	5	53	dep	75wt	692:695	arg1	fractions					673:681	various loading fractions	657:681	various loading fractions (25, 50	657:689	%) at various loading fractions (25, 50, 75wt.
26794733	5	53	dep	75wt	692:695	arg1	%					651:651	%	651:651	%	651:651	%) at various loading fractions (25, 50, 75wt.
26794733	2	54	theme	biopolymer	291:300	arg1	development					274:284	development	274:284	development of a biopolymer based on chitosan (CT) and halloysite nanotubes (HNT)	274:354	This study concentrates on development of a biopolymer based on chitosan (CT) and halloysite nanotubes (HNT) and evaluates the copper removal intake as a potential application of this bio-composite.
26794733	10	55	theme	Dragonite	1251:1259	arg1	source					1261:1266	Dragonite source	1251:1266	Dragonite source	1251:1266	The adsorption capacity of the CT/HNT beads prepared from Dragonite source had a larger adsorption capacity of 14.2mg/g as compared to that of Matauri Bay, 10.55mg/g.
26794733	1	56	theme	materials	151:159	arg1	Development					132:142	Development	132:142	Development of new materials for different applications especially as bio-composites	132:215	Development of new materials for different applications especially as bio-composites has received great attention.
26794733	12	57	dep	characteristics	1594:1608	arg1	the					1590:1592	the	1590:1592	the	1590:1592	The result of this study helps to understand the links between the characteristics and adsorption abilities of CT/HNT beads.
26794733	10	58	contain	had	1268:1270	arg1	capacity					1208:1215	The adsorption capacity	1193:1215	The adsorption capacity of the CT/HNT beads prepared from Dragonite source	1193:1266	The adsorption capacity of the CT/HNT beads prepared from Dragonite source had a larger adsorption capacity of 14.2mg/g as compared to that of Matauri Bay, 10.55mg/g.
26794733	10	58	contain	had	1268:1270	arg2	capacity					1292:1299	a larger adsorption capacity	1272:1299	a larger adsorption capacity of 14.2mg/g	1272:1311	The adsorption capacity of the CT/HNT beads prepared from Dragonite source had a larger adsorption capacity of 14.2mg/g as compared to that of Matauri Bay, 10.55mg/g.
26794733	0	59	theme	study	90:94	arg1	application					96:106	ultrasonic-assisted extrusion-dripping and a case study application	40:106	ultrasonic-assisted extrusion-dripping and a case study application for copper ion removal	40:129	Chitosan/halloysite beads fabricated by ultrasonic-assisted extrusion-dripping and a case study application for copper ion removal.
26794733	9	60	theme	limited	1150:1156	arg1	accessibility					1158:1170	limited accessibility	1150:1170	limited accessibility of the amino groups	1150:1190	% resulted in a decrease in adsorption capacity attributed to limited accessibility of the amino groups.
26794733	1	61	theme	great	230:234	arg1	attention					236:244	great attention	230:244	great attention	230:244	Development of new materials for different applications especially as bio-composites has received great attention.
26794733	2	62	theme	removal	381:387	arg1	intake					389:394	the copper removal intake	370:394	the copper removal intake	370:394	This study concentrates on development of a biopolymer based on chitosan (CT) and halloysite nanotubes (HNT) and evaluates the copper removal intake as a potential application of this bio-composite.
26794733	2	62	theme	removal	381:387	arg1	application					411:421	a potential application	399:421	a potential application of this bio-composite	399:443	This study concentrates on development of a biopolymer based on chitosan (CT) and halloysite nanotubes (HNT) and evaluates the copper removal intake as a potential application of this bio-composite.
26794733	7	63	theme	%	819:819	arg1	frequency					799:807	constant frequency	790:807	constant frequency of 25%, 50% and 75%	790:827	The effect of ultrasound as a mixing device was also studied by varying the amplitude at constant frequency of 25%, 50% and 75%.
26794733	0	64	theme	case	85:88	arg1	study					90:94	a case study	83:94	a case study	83:94	Chitosan/halloysite beads fabricated by ultrasonic-assisted extrusion-dripping and a case study application for copper ion removal.
26794733	11	65	theme	ultrasound	1505:1514	arg1	amplitude					1516:1524	constant ultrasound amplitude	1496:1524	constant ultrasound amplitude	1496:1524	It was observed that the adsorption capacity of the beads toward copper ions decreased when the loading fraction of HNT is increased at constant ultrasound amplitude.
26794733	4	66	theme	chitosan	628:635	arg1	solution					637:644	a chitosan solution	626:644	a chitosan solution (2wt	626:649	Two sources of HNTs (i.e. Dragonite and Matauri Bay) were added into a chitosan solution (2wt.
26794733	3	67	theme	first	545:549	arg1	time					551:554	the first time	541:554	the first time	541:554	In this study, CT/HNT beads were prepared by ultrasonic-assisted extrusion-dripping method for the first time.
26794733	7	68	theme	%	814:814	arg1	frequency					799:807	constant frequency	790:807	constant frequency of 25%, 50% and 75%	790:827	The effect of ultrasound as a mixing device was also studied by varying the amplitude at constant frequency of 25%, 50% and 75%.
26794733	5	69	dep	fractions	673:681	arg1	50					688:689	50	688:689	50	688:689	%) at various loading fractions (25, 50, 75wt.
29254032	8	0	theme	potent	1173:1178	arg1	SNPs					1145:1148	SNPs	1145:1148	SNPs	1145:1148	The above results prove that SNPs can be considered as a potent antibacterial and antioxidant drug in future.
29254032	8	0	theme	potent	1173:1178	arg1	drug					1210:1213	a potent antibacterial and antioxidant drug	1171:1213	a potent antibacterial and antioxidant drug in future	1171:1223	The above results prove that SNPs can be considered as a potent antibacterial and antioxidant drug in future.
29254032	3	1	theme	moisture	525:532	arg1	content					534:540	moisture content	525:540	moisture content (1.8%)	525:547	Exopolysaccharide of the Streptomyces violaceus composed of total carbohydrate (61.4%), ash (16.1%), moisture content (1.8%) and NMR confirmed their structural composition.
29254032	3	1	theme	moisture	525:532	arg1	%					546:546	1.8%	543:546	1.8%	543:546	Exopolysaccharide of the Streptomyces violaceus composed of total carbohydrate (61.4%), ash (16.1%), moisture content (1.8%) and NMR confirmed their structural composition.
29254032	0	2	theme	Streptomyces	123:134	arg1	violaceus					136:144	Streptomyces violaceus MM72	123:149	Streptomyces violaceus MM72	123:149	Characterization, antimicrobial and antioxidant property of exopolysaccharide mediated silver nanoparticles synthesized by Streptomyces violaceus MM72.
29254032	6	3	theme	antibacterial	935:947	arg1	activity					949:956	promising antibacterial activity	925:956	promising antibacterial activity	925:956	The SNPs has shown promising antibacterial activity were evaluated for MIC.
29254032	8	4	theme	antibacterial	1180:1192	arg1	SNPs					1145:1148	SNPs	1145:1148	SNPs	1145:1148	The above results prove that SNPs can be considered as a potent antibacterial and antioxidant drug in future.
29254032	8	4	theme	antibacterial	1180:1192	arg1	drug					1210:1213	a potent antibacterial and antioxidant drug	1171:1213	a potent antibacterial and antioxidant drug in future	1171:1223	The above results prove that SNPs can be considered as a potent antibacterial and antioxidant drug in future.
29254032	6	5	theme	promising	925:933	arg1	activity					949:956	promising antibacterial activity	925:956	promising antibacterial activity	925:956	The SNPs has shown promising antibacterial activity were evaluated for MIC.
29254032	2	6	theme	SNPs	386:389	arg1	exopolysaccharide					338:354	exopolysaccharide	338:354	exopolysaccharide	338:354	In this, we account the exopolysaccharide and its mediated synthesis of SNPs and their biological activities.
29254032	2	6	theme	SNPs	386:389	arg1	synthesis					373:381	its mediated synthesis	360:381	its mediated synthesis	360:381	In this, we account the exopolysaccharide and its mediated synthesis of SNPs and their biological activities.
29254032	7	7	contain	have	1060:1063	arg2	activity					1087:1094	promising antioxidant activity	1065:1094	promising antioxidant activity	1065:1094	Furthermore, the SNPs were tested for antioxidant activities and was found to have promising antioxidant activity over the standards.
29254032	7	7	contain	have	1060:1063	arg1	SNPs					999:1002	the SNPs	995:1002	the SNPs	995:1002	Furthermore, the SNPs were tested for antioxidant activities and was found to have promising antioxidant activity over the standards.
29254032	5	8	theme	diffusion	888:896	arg1	method					898:903	disc diffusion method	883:903	disc diffusion method	883:903	Further, the SNPs evaluated for its antibacterial activity against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis using disc diffusion method.
29254032	8	9	theme	antioxidant	1198:1208	arg1	SNPs					1145:1148	SNPs	1145:1148	SNPs	1145:1148	The above results prove that SNPs can be considered as a potent antibacterial and antioxidant drug in future.
29254032	8	9	theme	antioxidant	1198:1208	arg1	drug					1210:1213	a potent antibacterial and antioxidant drug	1171:1213	a potent antibacterial and antioxidant drug in future	1171:1223	The above results prove that SNPs can be considered as a potent antibacterial and antioxidant drug in future.
29254032	5	10	theme	antibacterial	760:772	arg1	activity					774:781	its antibacterial activity	756:781	its antibacterial activity against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis using disc diffusion method	756:903	Further, the SNPs evaluated for its antibacterial activity against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis using disc diffusion method.
29254032	3	11	theme	total	484:488	arg1	carbohydrate					490:501	total carbohydrate	484:501	total carbohydrate (61.4%)	484:509	Exopolysaccharide of the Streptomyces violaceus composed of total carbohydrate (61.4%), ash (16.1%), moisture content (1.8%) and NMR confirmed their structural composition.
29254032	3	11	theme	total	484:488	arg1	%					508:508	61.4%	504:508	61.4%	504:508	Exopolysaccharide of the Streptomyces violaceus composed of total carbohydrate (61.4%), ash (16.1%), moisture content (1.8%) and NMR confirmed their structural composition.
29254032	0	12	dep	nanoparticles	94:106	arg1	synthesized					108:118	synthesized	108:118	nanoparticles synthesized by Streptomyces violaceus MM72	94:149	Characterization, antimicrobial and antioxidant property of exopolysaccharide mediated silver nanoparticles synthesized by Streptomyces violaceus MM72.
29254032	1	13	theme	considerable	209:220	arg1	attention					222:230	considerable attention	209:230	considerable attention	209:230	Green synthesis of silver nanoparticle (SNPs) has gained considerable attention in nano-biotechnology and nano-medicine owing their potency and eco-friendliness.
29254032	2	14	theme	biological	401:410	arg1	activities					412:421	their biological activities	395:421	their biological activities	395:421	In this, we account the exopolysaccharide and its mediated synthesis of SNPs and their biological activities.
29254032	8	15	theme	above	1120:1124	arg1	results					1126:1132	The above results	1116:1132	The above results	1116:1132	The above results prove that SNPs can be considered as a potent antibacterial and antioxidant drug in future.
29254032	3	16	theme	structural	573:582	arg1	composition					584:594	their structural composition	567:594	their structural composition	567:594	Exopolysaccharide of the Streptomyces violaceus composed of total carbohydrate (61.4%), ash (16.1%), moisture content (1.8%) and NMR confirmed their structural composition.
29254032	4	17	theme	XRD	710:712	arg1	analyses					714:721	TEM and XRD analyses	702:721	TEM and XRD analyses	702:721	SNPs synthesized by the exopolysaccharide, confirmed using UV-vis spectral analysis and characterized by TEM and XRD analyses.
29254032	1	18	theme	silver	171:176	arg1	nanoparticle					178:189	silver nanoparticle	171:189	silver nanoparticle (SNPs)	171:196	Green synthesis of silver nanoparticle (SNPs) has gained considerable attention in nano-biotechnology and nano-medicine owing their potency and eco-friendliness.
29254032	1	18	theme	silver	171:176	arg1	SNPs					192:195	SNPs	192:195	SNPs	192:195	Green synthesis of silver nanoparticle (SNPs) has gained considerable attention in nano-biotechnology and nano-medicine owing their potency and eco-friendliness.
29254032	4	19	theme	spectral	663:670	arg1	analysis					672:679	UV-vis spectral analysis	656:679	UV-vis spectral analysis	656:679	SNPs synthesized by the exopolysaccharide, confirmed using UV-vis spectral analysis and characterized by TEM and XRD analyses.
29254032	0	20	theme	antioxidant	36:46	arg1	property					48:55	antioxidant property	36:55	antioxidant property	36:55	Characterization, antimicrobial and antioxidant property of exopolysaccharide mediated silver nanoparticles synthesized by Streptomyces violaceus MM72.
29254032	4	21	theme	UV-vis	656:661	arg1	analysis					672:679	UV-vis spectral analysis	656:679	UV-vis spectral analysis	656:679	SNPs synthesized by the exopolysaccharide, confirmed using UV-vis spectral analysis and characterized by TEM and XRD analyses.
29254032	3	22	theme	Streptomyces	449:460	arg1	violaceus					462:470	the Streptomyces violaceus	445:470	the Streptomyces violaceus	445:470	Exopolysaccharide of the Streptomyces violaceus composed of total carbohydrate (61.4%), ash (16.1%), moisture content (1.8%) and NMR confirmed their structural composition.
29254032	7	23	theme	antioxidant	1075:1085	arg1	activity					1087:1094	promising antioxidant activity	1065:1094	promising antioxidant activity	1065:1094	Furthermore, the SNPs were tested for antioxidant activities and was found to have promising antioxidant activity over the standards.
29254032	2	24	dep	exopolysaccharide	338:354	arg1	the					334:336	the	334:336	the	334:336	In this, we account the exopolysaccharide and its mediated synthesis of SNPs and their biological activities.
29254032	3	25	theme	violaceus	462:470	arg1	Exopolysaccharide					424:440	Exopolysaccharide	424:440	Exopolysaccharide of the Streptomyces violaceus composed of total carbohydrate (61.4%), ash (16.1%), moisture content (1.8%) and NMR	424:555	Exopolysaccharide of the Streptomyces violaceus composed of total carbohydrate (61.4%), ash (16.1%), moisture content (1.8%) and NMR confirmed their structural composition.
29254032	8	26	from	drug	1210:1213	arg1	future					1218:1223	future	1218:1223	future	1218:1223	The above results prove that SNPs can be considered as a potent antibacterial and antioxidant drug in future.
29254032	1	27	theme	nanoparticle	178:189	arg1	synthesis					158:166	Green synthesis	152:166	Green synthesis of silver nanoparticle (SNPs)	152:196	Green synthesis of silver nanoparticle (SNPs) has gained considerable attention in nano-biotechnology and nano-medicine owing their potency and eco-friendliness.
29254032	1	28	theme	Green	152:156	arg1	synthesis					158:166	Green synthesis	152:166	Green synthesis of silver nanoparticle (SNPs)	152:196	Green synthesis of silver nanoparticle (SNPs) has gained considerable attention in nano-biotechnology and nano-medicine owing their potency and eco-friendliness.
29254032	7	29	theme	promising	1065:1073	arg1	activity					1087:1094	promising antioxidant activity	1065:1094	promising antioxidant activity	1065:1094	Furthermore, the SNPs were tested for antioxidant activities and was found to have promising antioxidant activity over the standards.
29254032	7	30	theme	antioxidant	1020:1030	arg1	activities					1032:1041	antioxidant activities	1020:1041	antioxidant activities	1020:1041	Furthermore, the SNPs were tested for antioxidant activities and was found to have promising antioxidant activity over the standards.
29254032	2	31	theme	mediated	364:371	arg1	synthesis					373:381	its mediated synthesis	360:381	its mediated synthesis	360:381	In this, we account the exopolysaccharide and its mediated synthesis of SNPs and their biological activities.
29254032	0	32	theme	silver	87:92	arg1	antimicrobial					18:30	antimicrobial	18:30	antimicrobial	18:30	Characterization, antimicrobial and antioxidant property of exopolysaccharide mediated silver nanoparticles synthesized by Streptomyces violaceus MM72.
29254032	0	32	theme	silver	87:92	arg1	property					48:55	antioxidant property	36:55	antioxidant property	36:55	Characterization, antimicrobial and antioxidant property of exopolysaccharide mediated silver nanoparticles synthesized by Streptomyces violaceus MM72.
29254032	0	32	theme	silver	87:92	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization, antimicrobial and antioxidant property of exopolysaccharide mediated silver nanoparticles synthesized by Streptomyces violaceus MM72.
29254032	5	33	theme	disc	883:886	arg1	method					898:903	disc diffusion method	883:903	disc diffusion method	883:903	Further, the SNPs evaluated for its antibacterial activity against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis using disc diffusion method.
29254032	2	34	theme	activities	412:421	arg1	exopolysaccharide					338:354	exopolysaccharide	338:354	exopolysaccharide	338:354	In this, we account the exopolysaccharide and its mediated synthesis of SNPs and their biological activities.
29254032	2	34	theme	activities	412:421	arg1	synthesis					373:381	its mediated synthesis	360:381	its mediated synthesis	360:381	In this, we account the exopolysaccharide and its mediated synthesis of SNPs and their biological activities.
29254032	0	35	theme	mediated	78:85	arg1	silver					87:92	exopolysaccharide mediated silver	60:92	exopolysaccharide mediated silver	60:92	Characterization, antimicrobial and antioxidant property of exopolysaccharide mediated silver nanoparticles synthesized by Streptomyces violaceus MM72.
29254032	4	36	theme	TEM	702:704	arg1	analyses					714:721	TEM and XRD analyses	702:721	TEM and XRD analyses	702:721	SNPs synthesized by the exopolysaccharide, confirmed using UV-vis spectral analysis and characterized by TEM and XRD analyses.
26808461	4	0	theme	plant-inspired	982:995	arg1	architectures					997:1009	plant-inspired architectures	982:1009	plant-inspired architectures that change shape on immersion in water, yielding complex three-dimensional morphologies	982:1098	When combined with a minimal theoretical framework that allows us to solve the inverse problem of designing the alignment patterns for prescribed target shapes, we can programmably fabricate plant-inspired architectures that change shape on immersion in water, yielding complex three-dimensional morphologies.
26808461	1	1	theme	drug	142:145	arg1	delivery					147:154	drug delivery	142:154	drug delivery	142:154	Shape-morphing systems can be found in many areas, including smart textiles, autonomous robotics, biomedical devices, drug delivery and tissue engineering.
26808461	2	2	dep	composition	486:496	arg1	the					475:477	the	475:477	the	475:477	The natural analogues of such systems are exemplified by nastic plant motions, where a variety of organs such as tendrils, bracts, leaves and flowers respond to environmental stimuli (such as humidity, light or touch) by varying internal turgor, which leads to dynamic conformations governed by the tissue composition and microstructural anisotropy of cell walls.
26808461	2	3	theme	walls	537:541	arg1	composition					486:496	tissue composition	479:496	tissue composition	479:496	The natural analogues of such systems are exemplified by nastic plant motions, where a variety of organs such as tendrils, bracts, leaves and flowers respond to environmental stimuli (such as humidity, light or touch) by varying internal turgor, which leads to dynamic conformations governed by the tissue composition and microstructural anisotropy of cell walls.
26808461	2	3	theme	walls	537:541	arg1	anisotropy					518:527	microstructural anisotropy	502:527	microstructural anisotropy	502:527	The natural analogues of such systems are exemplified by nastic plant motions, where a variety of organs such as tendrils, bracts, leaves and flowers respond to environmental stimuli (such as humidity, light or touch) by varying internal turgor, which leads to dynamic conformations governed by the tissue composition and microstructural anisotropy of cell walls.
26808461	2	4	theme	cell	532:535	arg1	walls					537:541	cell walls	532:541	cell walls	532:541	The natural analogues of such systems are exemplified by nastic plant motions, where a variety of organs such as tendrils, bracts, leaves and flowers respond to environmental stimuli (such as humidity, light or touch) by varying internal turgor, which leads to dynamic conformations governed by the tissue composition and microstructural anisotropy of cell walls.
26808461	2	5	theme	dynamic	441:447	arg1	conformations					449:461	dynamic conformations	441:461	dynamic conformations governed by the tissue composition and microstructural anisotropy of cell walls	441:541	The natural analogues of such systems are exemplified by nastic plant motions, where a variety of organs such as tendrils, bracts, leaves and flowers respond to environmental stimuli (such as humidity, light or touch) by varying internal turgor, which leads to dynamic conformations governed by the tissue composition and microstructural anisotropy of cell walls.
26808461	4	6	theme	theoretical	820:830	arg1	framework					832:840	a minimal theoretical framework	810:840	a minimal theoretical framework that allows us to solve the inverse problem of designing the alignment patterns for prescribed target shapes	810:949	When combined with a minimal theoretical framework that allows us to solve the inverse problem of designing the alignment patterns for prescribed target shapes, we can programmably fabricate plant-inspired architectures that change shape on immersion in water, yielding complex three-dimensional morphologies.
26808461	1	7	theme	many	63:66	arg1	engineering					167:177	tissue engineering	160:177	tissue engineering	160:177	Shape-morphing systems can be found in many areas, including smart textiles, autonomous robotics, biomedical devices, drug delivery and tissue engineering.
26808461	1	7	theme	many	63:66	arg1	textiles					91:98	smart textiles	85:98	smart textiles	85:98	Shape-morphing systems can be found in many areas, including smart textiles, autonomous robotics, biomedical devices, drug delivery and tissue engineering.
26808461	1	7	theme	many	63:66	arg1	devices					133:139	biomedical devices	122:139	biomedical devices	122:139	Shape-morphing systems can be found in many areas, including smart textiles, autonomous robotics, biomedical devices, drug delivery and tissue engineering.
26808461	1	7	theme	many	63:66	arg1	areas					68:72	many areas	63:72	many areas	63:72	Shape-morphing systems can be found in many areas, including smart textiles, autonomous robotics, biomedical devices, drug delivery and tissue engineering.
26808461	1	7	theme	many	63:66	arg1	robotics					112:119	autonomous robotics	101:119	autonomous robotics	101:119	Shape-morphing systems can be found in many areas, including smart textiles, autonomous robotics, biomedical devices, drug delivery and tissue engineering.
26808461	1	7	theme	many	63:66	arg1	delivery					147:154	drug delivery	142:154	drug delivery	142:154	Shape-morphing systems can be found in many areas, including smart textiles, autonomous robotics, biomedical devices, drug delivery and tissue engineering.
26808461	4	8	theme	target	937:942	arg1	shapes					944:949	prescribed target shapes	926:949	prescribed target shapes	926:949	When combined with a minimal theoretical framework that allows us to solve the inverse problem of designing the alignment patterns for prescribed target shapes, we can programmably fabricate plant-inspired architectures that change shape on immersion in water, yielding complex three-dimensional morphologies.
26808461	4	9	theme	minimal	812:818	arg1	framework					832:840	a minimal theoretical framework	810:840	a minimal theoretical framework that allows us to solve the inverse problem of designing the alignment patterns for prescribed target shapes	810:949	When combined with a minimal theoretical framework that allows us to solve the inverse problem of designing the alignment patterns for prescribed target shapes, we can programmably fabricate plant-inspired architectures that change shape on immersion in water, yielding complex three-dimensional morphologies.
26808461	2	10	theme	environmental	341:353	arg1	stimuli					355:361	environmental stimuli	341:361	environmental stimuli (such as humidity, light or touch)	341:396	The natural analogues of such systems are exemplified by nastic plant motions, where a variety of organs such as tendrils, bracts, leaves and flowers respond to environmental stimuli (such as humidity, light or touch) by varying internal turgor, which leads to dynamic conformations governed by the tissue composition and microstructural anisotropy of cell walls.
26808461	0	11	theme	4D	11:12	arg1	printing					14:21	Biomimetic 4D printing	0:21	Biomimetic 4D printing.	0:22	Biomimetic 4D printing.
26808461	3	12	theme	swelling	670:677	arg1	behaviour					679:687	localized, anisotropic swelling behaviour	647:687	localized, anisotropic swelling behaviour controlled by the alignment of cellulose fibrils along prescribed four-dimensional printing pathways	647:788	Inspired by these botanical systems, we printed composite hydrogel architectures that are encoded with localized, anisotropic swelling behaviour controlled by the alignment of cellulose fibrils along prescribed four-dimensional printing pathways.
26808461	4	13	theme	prescribed	926:935	arg1	shapes					944:949	prescribed target shapes	926:949	prescribed target shapes	926:949	When combined with a minimal theoretical framework that allows us to solve the inverse problem of designing the alignment patterns for prescribed target shapes, we can programmably fabricate plant-inspired architectures that change shape on immersion in water, yielding complex three-dimensional morphologies.
26808461	1	14	theme	tissue	160:165	arg1	engineering					167:177	tissue engineering	160:177	tissue engineering	160:177	Shape-morphing systems can be found in many areas, including smart textiles, autonomous robotics, biomedical devices, drug delivery and tissue engineering.
26808461	0	15	theme	Biomimetic	0:9	arg1	printing					14:21	Biomimetic 4D printing	0:21	Biomimetic 4D printing.	0:22	Biomimetic 4D printing.
26808461	2	16	theme	organs	278:283	arg1	organs					278:283	organs	278:283	organs such as tendrils, bracts, leaves and flowers	278:328	The natural analogues of such systems are exemplified by nastic plant motions, where a variety of organs such as tendrils, bracts, leaves and flowers respond to environmental stimuli (such as humidity, light or touch) by varying internal turgor, which leads to dynamic conformations governed by the tissue composition and microstructural anisotropy of cell walls.
26808461	2	16	theme	organs	278:283	arg1	leaves					311:316	leaves	311:316	leaves	311:316	The natural analogues of such systems are exemplified by nastic plant motions, where a variety of organs such as tendrils, bracts, leaves and flowers respond to environmental stimuli (such as humidity, light or touch) by varying internal turgor, which leads to dynamic conformations governed by the tissue composition and microstructural anisotropy of cell walls.
26808461	2	16	theme	organs	278:283	arg1	flowers					322:328	flowers	322:328	flowers	322:328	The natural analogues of such systems are exemplified by nastic plant motions, where a variety of organs such as tendrils, bracts, leaves and flowers respond to environmental stimuli (such as humidity, light or touch) by varying internal turgor, which leads to dynamic conformations governed by the tissue composition and microstructural anisotropy of cell walls.
26808461	2	16	theme	organs	278:283	arg1	tendrils					293:300	tendrils	293:300	tendrils	293:300	The natural analogues of such systems are exemplified by nastic plant motions, where a variety of organs such as tendrils, bracts, leaves and flowers respond to environmental stimuli (such as humidity, light or touch) by varying internal turgor, which leads to dynamic conformations governed by the tissue composition and microstructural anisotropy of cell walls.
26808461	2	16	theme	organs	278:283	arg1	bracts					303:308	bracts	303:308	bracts	303:308	The natural analogues of such systems are exemplified by nastic plant motions, where a variety of organs such as tendrils, bracts, leaves and flowers respond to environmental stimuli (such as humidity, light or touch) by varying internal turgor, which leads to dynamic conformations governed by the tissue composition and microstructural anisotropy of cell walls.
26808461	2	16	theme	organs	278:283	arg1	variety					267:273	a variety	265:273	a variety of organs such as tendrils, bracts, leaves and flowers	265:328	The natural analogues of such systems are exemplified by nastic plant motions, where a variety of organs such as tendrils, bracts, leaves and flowers respond to environmental stimuli (such as humidity, light or touch) by varying internal turgor, which leads to dynamic conformations governed by the tissue composition and microstructural anisotropy of cell walls.
26808461	1	17	located	found	54:58	arg2	systems					39:45	Shape-morphing systems	24:45	Shape-morphing systems	24:45	Shape-morphing systems can be found in many areas, including smart textiles, autonomous robotics, biomedical devices, drug delivery and tissue engineering.
26808461	1	17	located	found	54:58	arg1	engineering					167:177	tissue engineering	160:177	tissue engineering	160:177	Shape-morphing systems can be found in many areas, including smart textiles, autonomous robotics, biomedical devices, drug delivery and tissue engineering.
26808461	1	17	located	found	54:58	arg1	textiles					91:98	smart textiles	85:98	smart textiles	85:98	Shape-morphing systems can be found in many areas, including smart textiles, autonomous robotics, biomedical devices, drug delivery and tissue engineering.
26808461	1	17	located	found	54:58	arg1	devices					133:139	biomedical devices	122:139	biomedical devices	122:139	Shape-morphing systems can be found in many areas, including smart textiles, autonomous robotics, biomedical devices, drug delivery and tissue engineering.
26808461	1	17	located	found	54:58	arg1	areas					68:72	many areas	63:72	many areas	63:72	Shape-morphing systems can be found in many areas, including smart textiles, autonomous robotics, biomedical devices, drug delivery and tissue engineering.
26808461	1	17	located	found	54:58	arg1	robotics					112:119	autonomous robotics	101:119	autonomous robotics	101:119	Shape-morphing systems can be found in many areas, including smart textiles, autonomous robotics, biomedical devices, drug delivery and tissue engineering.
26808461	1	17	located	found	54:58	arg1	delivery					147:154	drug delivery	142:154	drug delivery	142:154	Shape-morphing systems can be found in many areas, including smart textiles, autonomous robotics, biomedical devices, drug delivery and tissue engineering.
26808461	1	18	theme	smart	85:89	arg1	textiles					91:98	smart textiles	85:98	smart textiles	85:98	Shape-morphing systems can be found in many areas, including smart textiles, autonomous robotics, biomedical devices, drug delivery and tissue engineering.
26808461	3	19	dep	localized	647:655	arg1	anisotropic					658:668	anisotropic	658:668	anisotropic	658:668	Inspired by these botanical systems, we printed composite hydrogel architectures that are encoded with localized, anisotropic swelling behaviour controlled by the alignment of cellulose fibrils along prescribed four-dimensional printing pathways.
26808461	2	20	theme	tissue	479:484	arg1	composition					486:496	tissue composition	479:496	tissue composition	479:496	The natural analogues of such systems are exemplified by nastic plant motions, where a variety of organs such as tendrils, bracts, leaves and flowers respond to environmental stimuli (such as humidity, light or touch) by varying internal turgor, which leads to dynamic conformations governed by the tissue composition and microstructural anisotropy of cell walls.
26808461	2	21	theme	internal	409:416	arg1	turgor					418:423	internal turgor	409:423	internal turgor	409:423	The natural analogues of such systems are exemplified by nastic plant motions, where a variety of organs such as tendrils, bracts, leaves and flowers respond to environmental stimuli (such as humidity, light or touch) by varying internal turgor, which leads to dynamic conformations governed by the tissue composition and microstructural anisotropy of cell walls.
26808461	3	22	theme	botanical	562:570	arg1	systems					572:578	these botanical systems	556:578	these botanical systems	556:578	Inspired by these botanical systems, we printed composite hydrogel architectures that are encoded with localized, anisotropic swelling behaviour controlled by the alignment of cellulose fibrils along prescribed four-dimensional printing pathways.
26808461	3	23	theme	composite	592:600	arg1	architectures					611:623	composite hydrogel architectures	592:623	composite hydrogel architectures that are encoded with localized, anisotropic swelling behaviour controlled by the alignment of cellulose fibrils along prescribed four-dimensional printing pathways	592:788	Inspired by these botanical systems, we printed composite hydrogel architectures that are encoded with localized, anisotropic swelling behaviour controlled by the alignment of cellulose fibrils along prescribed four-dimensional printing pathways.
26808461	4	24	theme	alignment	903:911	arg1	patterns					913:920	the alignment patterns	899:920	the alignment patterns for prescribed target shapes	899:949	When combined with a minimal theoretical framework that allows us to solve the inverse problem of designing the alignment patterns for prescribed target shapes, we can programmably fabricate plant-inspired architectures that change shape on immersion in water, yielding complex three-dimensional morphologies.
26808461	2	25	theme	systems	210:216	arg1	analogues					192:200	The natural analogues	180:200	The natural analogues of such systems	180:216	The natural analogues of such systems are exemplified by nastic plant motions, where a variety of organs such as tendrils, bracts, leaves and flowers respond to environmental stimuli (such as humidity, light or touch) by varying internal turgor, which leads to dynamic conformations governed by the tissue composition and microstructural anisotropy of cell walls.
26808461	3	26	theme	prescribed	744:753	arg1	pathways					781:788	prescribed four-dimensional printing pathways	744:788	prescribed four-dimensional printing pathways	744:788	Inspired by these botanical systems, we printed composite hydrogel architectures that are encoded with localized, anisotropic swelling behaviour controlled by the alignment of cellulose fibrils along prescribed four-dimensional printing pathways.
26808461	4	27	theme	inverse	870:876	arg1	problem					878:884	the inverse problem	866:884	the inverse problem of designing the alignment patterns for prescribed target shapes	866:949	When combined with a minimal theoretical framework that allows us to solve the inverse problem of designing the alignment patterns for prescribed target shapes, we can programmably fabricate plant-inspired architectures that change shape on immersion in water, yielding complex three-dimensional morphologies.
26808461	2	28	theme	such	205:208	arg1	systems					210:216	such systems	205:216	such systems	205:216	The natural analogues of such systems are exemplified by nastic plant motions, where a variety of organs such as tendrils, bracts, leaves and flowers respond to environmental stimuli (such as humidity, light or touch) by varying internal turgor, which leads to dynamic conformations governed by the tissue composition and microstructural anisotropy of cell walls.
26808461	3	29	theme	cellulose	720:728	arg1	fibrils					730:736	cellulose fibrils	720:736	cellulose fibrils	720:736	Inspired by these botanical systems, we printed composite hydrogel architectures that are encoded with localized, anisotropic swelling behaviour controlled by the alignment of cellulose fibrils along prescribed four-dimensional printing pathways.
26808461	2	30	theme	natural	184:190	arg1	analogues					192:200	The natural analogues	180:200	The natural analogues of such systems	180:216	The natural analogues of such systems are exemplified by nastic plant motions, where a variety of organs such as tendrils, bracts, leaves and flowers respond to environmental stimuli (such as humidity, light or touch) by varying internal turgor, which leads to dynamic conformations governed by the tissue composition and microstructural anisotropy of cell walls.
26808461	1	31	theme	autonomous	101:110	arg1	robotics					112:119	autonomous robotics	101:119	autonomous robotics	101:119	Shape-morphing systems can be found in many areas, including smart textiles, autonomous robotics, biomedical devices, drug delivery and tissue engineering.
26808461	4	32	theme	three-dimensional	1069:1085	arg1	morphologies					1087:1098	complex three-dimensional morphologies	1061:1098	complex three-dimensional morphologies	1061:1098	When combined with a minimal theoretical framework that allows us to solve the inverse problem of designing the alignment patterns for prescribed target shapes, we can programmably fabricate plant-inspired architectures that change shape on immersion in water, yielding complex three-dimensional morphologies.
26808461	3	33	theme	fibrils	730:736	arg1	alignment					707:715	the alignment	703:715	the alignment of cellulose fibrils along prescribed four-dimensional printing pathways	703:788	Inspired by these botanical systems, we printed composite hydrogel architectures that are encoded with localized, anisotropic swelling behaviour controlled by the alignment of cellulose fibrils along prescribed four-dimensional printing pathways.
26808461	2	34	theme	microstructural	502:516	arg1	anisotropy					518:527	microstructural anisotropy	502:527	microstructural anisotropy	502:527	The natural analogues of such systems are exemplified by nastic plant motions, where a variety of organs such as tendrils, bracts, leaves and flowers respond to environmental stimuli (such as humidity, light or touch) by varying internal turgor, which leads to dynamic conformations governed by the tissue composition and microstructural anisotropy of cell walls.
26808461	1	35	theme	Shape-morphing	24:37	arg1	systems					39:45	Shape-morphing systems	24:45	Shape-morphing systems	24:45	Shape-morphing systems can be found in many areas, including smart textiles, autonomous robotics, biomedical devices, drug delivery and tissue engineering.
26808461	2	36	theme	plant	244:248	arg1	motions					250:256	nastic plant motions	237:256	nastic plant motions	237:256	The natural analogues of such systems are exemplified by nastic plant motions, where a variety of organs such as tendrils, bracts, leaves and flowers respond to environmental stimuli (such as humidity, light or touch) by varying internal turgor, which leads to dynamic conformations governed by the tissue composition and microstructural anisotropy of cell walls.
26808461	4	37	theme	complex	1061:1067	arg1	morphologies					1087:1098	complex three-dimensional morphologies	1061:1098	complex three-dimensional morphologies	1061:1098	When combined with a minimal theoretical framework that allows us to solve the inverse problem of designing the alignment patterns for prescribed target shapes, we can programmably fabricate plant-inspired architectures that change shape on immersion in water, yielding complex three-dimensional morphologies.
26808461	3	38	theme	hydrogel	602:609	arg1	architectures					611:623	composite hydrogel architectures	592:623	composite hydrogel architectures that are encoded with localized, anisotropic swelling behaviour controlled by the alignment of cellulose fibrils along prescribed four-dimensional printing pathways	592:788	Inspired by these botanical systems, we printed composite hydrogel architectures that are encoded with localized, anisotropic swelling behaviour controlled by the alignment of cellulose fibrils along prescribed four-dimensional printing pathways.
26808461	2	39	theme	nastic	237:242	arg1	motions					250:256	nastic plant motions	237:256	nastic plant motions	237:256	The natural analogues of such systems are exemplified by nastic plant motions, where a variety of organs such as tendrils, bracts, leaves and flowers respond to environmental stimuli (such as humidity, light or touch) by varying internal turgor, which leads to dynamic conformations governed by the tissue composition and microstructural anisotropy of cell walls.
26808461	2	40	dep	stimuli	355:361	arg1	such					364:367	such	364:367	such	364:367	The natural analogues of such systems are exemplified by nastic plant motions, where a variety of organs such as tendrils, bracts, leaves and flowers respond to environmental stimuli (such as humidity, light or touch) by varying internal turgor, which leads to dynamic conformations governed by the tissue composition and microstructural anisotropy of cell walls.
26808461	1	41	theme	biomedical	122:131	arg1	devices					133:139	biomedical devices	122:139	biomedical devices	122:139	Shape-morphing systems can be found in many areas, including smart textiles, autonomous robotics, biomedical devices, drug delivery and tissue engineering.
26808461	3	42	theme	four-dimensional	755:770	arg1	pathways					781:788	prescribed four-dimensional printing pathways	744:788	prescribed four-dimensional printing pathways	744:788	Inspired by these botanical systems, we printed composite hydrogel architectures that are encoded with localized, anisotropic swelling behaviour controlled by the alignment of cellulose fibrils along prescribed four-dimensional printing pathways.
26808461	3	43	theme	localized	647:655	arg1	behaviour					679:687	localized, anisotropic swelling behaviour	647:687	localized, anisotropic swelling behaviour controlled by the alignment of cellulose fibrils along prescribed four-dimensional printing pathways	647:788	Inspired by these botanical systems, we printed composite hydrogel architectures that are encoded with localized, anisotropic swelling behaviour controlled by the alignment of cellulose fibrils along prescribed four-dimensional printing pathways.
26808461	3	44	theme	printing	772:779	arg1	pathways					781:788	prescribed four-dimensional printing pathways	744:788	prescribed four-dimensional printing pathways	744:788	Inspired by these botanical systems, we printed composite hydrogel architectures that are encoded with localized, anisotropic swelling behaviour controlled by the alignment of cellulose fibrils along prescribed four-dimensional printing pathways.
27246379	0	0	theme	cellulose	82:90	arg1	biomaterials					45:56	integrated biomaterials	34:56	integrated biomaterials of sodium carboxymethyl cellulose and chitosan	34:103	Removal of toxic indigo blue with integrated biomaterials of sodium carboxymethyl cellulose and chitosan.
27246379	6	1	theme	textile	1244:1250	arg1	wastewater					1252:1261	textile wastewater	1244:1261	textile wastewater	1244:1261	It opens a new avenue for the effective and convenient treatment of textile wastewater with integrated natural biopolymers.
27246379	3	2	theme	mass	634:637	arg1	fraction					639:646	The mass fraction	630:646	The mass fraction of CS in the CMC-CS-A	630:668	The mass fraction of CS in the CMC-CS-A is also investigated to achieve the best adsorption of IB, and it reveals that satisfactory removal of IB can be achieved at the CMC-CS-A (50wt%).
27246379	5	3	theme	isotherm	1118:1125	arg1	model					1127:1131	the Langmuir isotherm model	1105:1131	the Langmuir isotherm model	1105:1131	Finally, adsorption isotherms and adsorption kinetics are investigated and compared by using classic models, and the results indicate that the adsorption fits well with the Langmuir isotherm model and the pseudo-second-order kinetic model.
27246379	0	4	theme	carboxymethyl	68:80	arg1	cellulose					82:90	sodium carboxymethyl cellulose	61:90	sodium carboxymethyl cellulose	61:90	Removal of toxic indigo blue with integrated biomaterials of sodium carboxymethyl cellulose and chitosan.
27246379	2	5	theme	interconnected	561:574	arg1	structure					591:599	the unique interconnected porous network structure	550:599	the unique interconnected porous network structure of the as-prepared CMC-CS-A	550:627	The results of control experiments indicate that the adsorption performance of CMC-CS-A is superior to that of NaCMC, CS, and the composites of NaCMC and CS integrated via electrostatic attractions (CMC-CS-E), which can be attributed to the unique interconnected porous network structure of the as-prepared CMC-CS-A.
27246379	0	6	theme	chitosan	96:103	arg1	biomaterials					45:56	integrated biomaterials	34:56	integrated biomaterials of sodium carboxymethyl cellulose and chitosan	34:103	Removal of toxic indigo blue with integrated biomaterials of sodium carboxymethyl cellulose and chitosan.
27246379	2	7	theme	unique	554:559	arg1	structure					591:599	the unique interconnected porous network structure	550:599	the unique interconnected porous network structure of the as-prepared CMC-CS-A	550:627	The results of control experiments indicate that the adsorption performance of CMC-CS-A is superior to that of NaCMC, CS, and the composites of NaCMC and CS integrated via electrostatic attractions (CMC-CS-E), which can be attributed to the unique interconnected porous network structure of the as-prepared CMC-CS-A.
27246379	4	8	theme	contact	846:852	arg1	time					854:857	contact time	846:857	contact time	846:857	Important parameters such as contact time, temperature and initial concentration are discussed in detail in this work.
27246379	6	9	theme	convenient	1220:1229	arg1	treatment					1231:1239	the effective and convenient treatment	1202:1239	the effective and convenient treatment of textile wastewater with integrated natural biopolymers	1202:1297	It opens a new avenue for the effective and convenient treatment of textile wastewater with integrated natural biopolymers.
27246379	3	10	theme	IB	725:726	arg1	adsorption					711:720	the best adsorption	702:720	the best adsorption of IB	702:726	The mass fraction of CS in the CMC-CS-A is also investigated to achieve the best adsorption of IB, and it reveals that satisfactory removal of IB can be achieved at the CMC-CS-A (50wt%).
27246379	1	11	theme	first	301:305	arg1	time					307:310	the first time	297:310	the first time	297:310	Sodium carboxymethyl cellulose (NaCMC) and chitosan (CS) are successfully combined via amidation, and the obtained product, CMC-CS-A, is used for the adsorption of toxic indigo blue (IB) for the first time.
27246379	2	12	theme	control	328:334	arg1	experiments					336:346	control experiments	328:346	control experiments	328:346	The results of control experiments indicate that the adsorption performance of CMC-CS-A is superior to that of NaCMC, CS, and the composites of NaCMC and CS integrated via electrostatic attractions (CMC-CS-E), which can be attributed to the unique interconnected porous network structure of the as-prepared CMC-CS-A.
27246379	4	13	theme	Important	817:825	arg1	parameters					827:836	Important parameters	817:836	Important parameters such as contact time, temperature and initial concentration	817:896	Important parameters such as contact time, temperature and initial concentration are discussed in detail in this work.
27246379	4	13	theme	Important	817:825	arg1	temperature					860:870	temperature	860:870	temperature	860:870	Important parameters such as contact time, temperature and initial concentration are discussed in detail in this work.
27246379	4	13	theme	Important	817:825	arg1	concentration					884:896	initial concentration	876:896	initial concentration	876:896	Important parameters such as contact time, temperature and initial concentration are discussed in detail in this work.
27246379	4	13	theme	Important	817:825	arg1	time					854:857	contact time	846:857	contact time	846:857	Important parameters such as contact time, temperature and initial concentration are discussed in detail in this work.
27246379	5	14	theme	adsorption	970:979	arg1	kinetics					981:988	adsorption kinetics	970:988	adsorption kinetics	970:988	Finally, adsorption isotherms and adsorption kinetics are investigated and compared by using classic models, and the results indicate that the adsorption fits well with the Langmuir isotherm model and the pseudo-second-order kinetic model.
27246379	6	15	theme	effective	1206:1214	arg1	treatment					1231:1239	the effective and convenient treatment	1202:1239	the effective and convenient treatment of textile wastewater with integrated natural biopolymers	1202:1297	It opens a new avenue for the effective and convenient treatment of textile wastewater with integrated natural biopolymers.
27246379	2	16	theme	CMC-CS-A	392:399	arg1	superior					404:411	superior	404:411	superior	404:411	The results of control experiments indicate that the adsorption performance of CMC-CS-A is superior to that of NaCMC, CS, and the composites of NaCMC and CS integrated via electrostatic attractions (CMC-CS-E), which can be attributed to the unique interconnected porous network structure of the as-prepared CMC-CS-A.
27246379	2	16	theme	CMC-CS-A	392:399	arg1	performance					377:387	the adsorption performance	362:387	the adsorption performance of CMC-CS-A	362:399	The results of control experiments indicate that the adsorption performance of CMC-CS-A is superior to that of NaCMC, CS, and the composites of NaCMC and CS integrated via electrostatic attractions (CMC-CS-E), which can be attributed to the unique interconnected porous network structure of the as-prepared CMC-CS-A.
27246379	1	17	used	used	243:246	arg2	CMC-CS-A					230:237	CMC-CS-A	230:237	CMC-CS-A	230:237	Sodium carboxymethyl cellulose (NaCMC) and chitosan (CS) are successfully combined via amidation, and the obtained product, CMC-CS-A, is used for the adsorption of toxic indigo blue (IB) for the first time.
27246379	1	17	used	used	243:246	arg2	product					221:227	the obtained product	208:227	the obtained product	208:227	Sodium carboxymethyl cellulose (NaCMC) and chitosan (CS) are successfully combined via amidation, and the obtained product, CMC-CS-A, is used for the adsorption of toxic indigo blue (IB) for the first time.
27246379	6	18	theme	natural	1279:1285	arg1	biopolymers					1287:1297	integrated natural biopolymers	1268:1297	integrated natural biopolymers	1268:1297	It opens a new avenue for the effective and convenient treatment of textile wastewater with integrated natural biopolymers.
27246379	2	19	theme	network	583:589	arg1	structure					591:599	the unique interconnected porous network structure	550:599	the unique interconnected porous network structure of the as-prepared CMC-CS-A	550:627	The results of control experiments indicate that the adsorption performance of CMC-CS-A is superior to that of NaCMC, CS, and the composites of NaCMC and CS integrated via electrostatic attractions (CMC-CS-E), which can be attributed to the unique interconnected porous network structure of the as-prepared CMC-CS-A.
27246379	3	20	from	fraction	639:646	arg1	CMC-CS-A					661:668	the CMC-CS-A	657:668	the CMC-CS-A	657:668	The mass fraction of CS in the CMC-CS-A is also investigated to achieve the best adsorption of IB, and it reveals that satisfactory removal of IB can be achieved at the CMC-CS-A (50wt%).
27246379	2	21	theme	CS	467:468	arg1	CS					467:468	CS	467:468	CS	467:468	The results of control experiments indicate that the adsorption performance of CMC-CS-A is superior to that of NaCMC, CS, and the composites of NaCMC and CS integrated via electrostatic attractions (CMC-CS-E), which can be attributed to the unique interconnected porous network structure of the as-prepared CMC-CS-A.
27246379	2	21	theme	CS	467:468	arg1	CS					431:432	CS	431:432	CS	431:432	The results of control experiments indicate that the adsorption performance of CMC-CS-A is superior to that of NaCMC, CS, and the composites of NaCMC and CS integrated via electrostatic attractions (CMC-CS-E), which can be attributed to the unique interconnected porous network structure of the as-prepared CMC-CS-A.
27246379	2	21	theme	CS	467:468	arg1	NaCMC					457:461	NaCMC	457:461	NaCMC	457:461	The results of control experiments indicate that the adsorption performance of CMC-CS-A is superior to that of NaCMC, CS, and the composites of NaCMC and CS integrated via electrostatic attractions (CMC-CS-E), which can be attributed to the unique interconnected porous network structure of the as-prepared CMC-CS-A.
27246379	2	21	theme	CS	467:468	arg1	NaCMC					424:428	NaCMC	424:428	NaCMC	424:428	The results of control experiments indicate that the adsorption performance of CMC-CS-A is superior to that of NaCMC, CS, and the composites of NaCMC and CS integrated via electrostatic attractions (CMC-CS-E), which can be attributed to the unique interconnected porous network structure of the as-prepared CMC-CS-A.
27246379	2	21	theme	CS	467:468	arg1	composites					443:452	the composites	439:452	the composites of NaCMC and CS integrated via electrostatic attractions (CMC-CS-E), which can be attributed to the unique interconnected porous network structure of the as-prepared CMC-CS-A	439:627	The results of control experiments indicate that the adsorption performance of CMC-CS-A is superior to that of NaCMC, CS, and the composites of NaCMC and CS integrated via electrostatic attractions (CMC-CS-E), which can be attributed to the unique interconnected porous network structure of the as-prepared CMC-CS-A.
27246379	2	22	theme	porous	576:581	arg1	structure					591:599	the unique interconnected porous network structure	550:599	the unique interconnected porous network structure of the as-prepared CMC-CS-A	550:627	The results of control experiments indicate that the adsorption performance of CMC-CS-A is superior to that of NaCMC, CS, and the composites of NaCMC and CS integrated via electrostatic attractions (CMC-CS-E), which can be attributed to the unique interconnected porous network structure of the as-prepared CMC-CS-A.
27246379	2	23	theme	adsorption	366:375	arg1	superior					404:411	superior	404:411	superior	404:411	The results of control experiments indicate that the adsorption performance of CMC-CS-A is superior to that of NaCMC, CS, and the composites of NaCMC and CS integrated via electrostatic attractions (CMC-CS-E), which can be attributed to the unique interconnected porous network structure of the as-prepared CMC-CS-A.
27246379	2	23	theme	adsorption	366:375	arg1	performance					377:387	the adsorption performance	362:387	the adsorption performance of CMC-CS-A	362:399	The results of control experiments indicate that the adsorption performance of CMC-CS-A is superior to that of NaCMC, CS, and the composites of NaCMC and CS integrated via electrostatic attractions (CMC-CS-E), which can be attributed to the unique interconnected porous network structure of the as-prepared CMC-CS-A.
27246379	3	24	theme	CS	651:652	arg1	fraction					639:646	The mass fraction	630:646	The mass fraction of CS in the CMC-CS-A	630:668	The mass fraction of CS in the CMC-CS-A is also investigated to achieve the best adsorption of IB, and it reveals that satisfactory removal of IB can be achieved at the CMC-CS-A (50wt%).
27246379	1	25	theme	Sodium	106:111	arg1	NaCMC					138:142	NaCMC	138:142	NaCMC	138:142	Sodium carboxymethyl cellulose (NaCMC) and chitosan (CS) are successfully combined via amidation, and the obtained product, CMC-CS-A, is used for the adsorption of toxic indigo blue (IB) for the first time.
27246379	1	25	theme	Sodium	106:111	arg1	cellulose					127:135	Sodium carboxymethyl cellulose	106:135	Sodium carboxymethyl cellulose (NaCMC)	106:143	Sodium carboxymethyl cellulose (NaCMC) and chitosan (CS) are successfully combined via amidation, and the obtained product, CMC-CS-A, is used for the adsorption of toxic indigo blue (IB) for the first time.
27246379	2	26	theme	NaCMC	457:461	arg1	CS					467:468	CS	467:468	CS	467:468	The results of control experiments indicate that the adsorption performance of CMC-CS-A is superior to that of NaCMC, CS, and the composites of NaCMC and CS integrated via electrostatic attractions (CMC-CS-E), which can be attributed to the unique interconnected porous network structure of the as-prepared CMC-CS-A.
27246379	2	26	theme	NaCMC	457:461	arg1	CS					431:432	CS	431:432	CS	431:432	The results of control experiments indicate that the adsorption performance of CMC-CS-A is superior to that of NaCMC, CS, and the composites of NaCMC and CS integrated via electrostatic attractions (CMC-CS-E), which can be attributed to the unique interconnected porous network structure of the as-prepared CMC-CS-A.
27246379	2	26	theme	NaCMC	457:461	arg1	NaCMC					457:461	NaCMC	457:461	NaCMC	457:461	The results of control experiments indicate that the adsorption performance of CMC-CS-A is superior to that of NaCMC, CS, and the composites of NaCMC and CS integrated via electrostatic attractions (CMC-CS-E), which can be attributed to the unique interconnected porous network structure of the as-prepared CMC-CS-A.
27246379	2	26	theme	NaCMC	457:461	arg1	NaCMC					424:428	NaCMC	424:428	NaCMC	424:428	The results of control experiments indicate that the adsorption performance of CMC-CS-A is superior to that of NaCMC, CS, and the composites of NaCMC and CS integrated via electrostatic attractions (CMC-CS-E), which can be attributed to the unique interconnected porous network structure of the as-prepared CMC-CS-A.
27246379	2	26	theme	NaCMC	457:461	arg1	composites					443:452	the composites	439:452	the composites of NaCMC and CS integrated via electrostatic attractions (CMC-CS-E), which can be attributed to the unique interconnected porous network structure of the as-prepared CMC-CS-A	439:627	The results of control experiments indicate that the adsorption performance of CMC-CS-A is superior to that of NaCMC, CS, and the composites of NaCMC and CS integrated via electrostatic attractions (CMC-CS-E), which can be attributed to the unique interconnected porous network structure of the as-prepared CMC-CS-A.
27246379	0	27	theme	blue	24:27	arg1	Removal					0:6	Removal	0:6	Removal of toxic indigo blue with integrated biomaterials of sodium carboxymethyl cellulose and chitosan.	0:104	Removal of toxic indigo blue with integrated biomaterials of sodium carboxymethyl cellulose and chitosan.
27246379	6	28	theme	new	1187:1189	arg1	avenue					1191:1196	a new avenue	1185:1196	a new avenue for the effective and convenient treatment of textile wastewater with integrated natural biopolymers	1185:1297	It opens a new avenue for the effective and convenient treatment of textile wastewater with integrated natural biopolymers.
27246379	2	29	theme	experiments	336:346	arg1	results					317:323	The results	313:323	The results of control experiments	313:346	The results of control experiments indicate that the adsorption performance of CMC-CS-A is superior to that of NaCMC, CS, and the composites of NaCMC and CS integrated via electrostatic attractions (CMC-CS-E), which can be attributed to the unique interconnected porous network structure of the as-prepared CMC-CS-A.
27246379	4	30	theme	initial	876:882	arg1	concentration					884:896	initial concentration	876:896	initial concentration	876:896	Important parameters such as contact time, temperature and initial concentration are discussed in detail in this work.
27246379	5	31	theme	adsorption	945:954	arg1	isotherms					956:964	adsorption isotherms	945:964	adsorption isotherms	945:964	Finally, adsorption isotherms and adsorption kinetics are investigated and compared by using classic models, and the results indicate that the adsorption fits well with the Langmuir isotherm model and the pseudo-second-order kinetic model.
27246379	2	32	theme	CMC-CS-A	620:627	arg1	structure					591:599	the unique interconnected porous network structure	550:599	the unique interconnected porous network structure of the as-prepared CMC-CS-A	550:627	The results of control experiments indicate that the adsorption performance of CMC-CS-A is superior to that of NaCMC, CS, and the composites of NaCMC and CS integrated via electrostatic attractions (CMC-CS-E), which can be attributed to the unique interconnected porous network structure of the as-prepared CMC-CS-A.
27246379	1	33	theme	carboxymethyl	113:125	arg1	NaCMC					138:142	NaCMC	138:142	NaCMC	138:142	Sodium carboxymethyl cellulose (NaCMC) and chitosan (CS) are successfully combined via amidation, and the obtained product, CMC-CS-A, is used for the adsorption of toxic indigo blue (IB) for the first time.
27246379	1	33	theme	carboxymethyl	113:125	arg1	cellulose					127:135	Sodium carboxymethyl cellulose	106:135	Sodium carboxymethyl cellulose (NaCMC)	106:143	Sodium carboxymethyl cellulose (NaCMC) and chitosan (CS) are successfully combined via amidation, and the obtained product, CMC-CS-A, is used for the adsorption of toxic indigo blue (IB) for the first time.
27246379	0	34	theme	integrated	34:43	arg1	biomaterials					45:56	integrated biomaterials	34:56	integrated biomaterials of sodium carboxymethyl cellulose and chitosan	34:103	Removal of toxic indigo blue with integrated biomaterials of sodium carboxymethyl cellulose and chitosan.
27246379	5	35	theme	classic	1029:1035	arg1	models					1037:1042	classic models	1029:1042	classic models	1029:1042	Finally, adsorption isotherms and adsorption kinetics are investigated and compared by using classic models, and the results indicate that the adsorption fits well with the Langmuir isotherm model and the pseudo-second-order kinetic model.
27246379	2	36	theme	electrostatic	485:497	arg1	CMC-CS-E					512:519	CMC-CS-E	512:519	CMC-CS-E	512:519	The results of control experiments indicate that the adsorption performance of CMC-CS-A is superior to that of NaCMC, CS, and the composites of NaCMC and CS integrated via electrostatic attractions (CMC-CS-E), which can be attributed to the unique interconnected porous network structure of the as-prepared CMC-CS-A.
27246379	2	36	theme	electrostatic	485:497	arg1	attractions					499:509	electrostatic attractions	485:509	electrostatic attractions (CMC-CS-E)	485:520	The results of control experiments indicate that the adsorption performance of CMC-CS-A is superior to that of NaCMC, CS, and the composites of NaCMC and CS integrated via electrostatic attractions (CMC-CS-E), which can be attributed to the unique interconnected porous network structure of the as-prepared CMC-CS-A.
27246379	2	37	theme	as-prepared	608:618	arg1	CMC-CS-A					620:627	the as-prepared CMC-CS-A	604:627	the as-prepared CMC-CS-A	604:627	The results of control experiments indicate that the adsorption performance of CMC-CS-A is superior to that of NaCMC, CS, and the composites of NaCMC and CS integrated via electrostatic attractions (CMC-CS-E), which can be attributed to the unique interconnected porous network structure of the as-prepared CMC-CS-A.
27246379	1	38	theme	blue	283:286	arg1	adsorption					256:265	the adsorption	252:265	the adsorption of toxic indigo blue (IB)	252:291	Sodium carboxymethyl cellulose (NaCMC) and chitosan (CS) are successfully combined via amidation, and the obtained product, CMC-CS-A, is used for the adsorption of toxic indigo blue (IB) for the first time.
27246379	0	39	with	Removal	0:6	arg1	biomaterials					45:56	integrated biomaterials	34:56	integrated biomaterials of sodium carboxymethyl cellulose and chitosan	34:103	Removal of toxic indigo blue with integrated biomaterials of sodium carboxymethyl cellulose and chitosan.
27246379	3	40	theme	satisfactory	749:760	arg1	removal					762:768	satisfactory removal	749:768	satisfactory removal of IB	749:774	The mass fraction of CS in the CMC-CS-A is also investigated to achieve the best adsorption of IB, and it reveals that satisfactory removal of IB can be achieved at the CMC-CS-A (50wt%).
27246379	3	41	theme	best	706:709	arg1	adsorption					711:720	the best adsorption	702:720	the best adsorption of IB	702:726	The mass fraction of CS in the CMC-CS-A is also investigated to achieve the best adsorption of IB, and it reveals that satisfactory removal of IB can be achieved at the CMC-CS-A (50wt%).
27246379	5	42	theme	kinetic	1161:1167	arg1	model					1169:1173	the pseudo-second-order kinetic model	1137:1173	the pseudo-second-order kinetic model	1137:1173	Finally, adsorption isotherms and adsorption kinetics are investigated and compared by using classic models, and the results indicate that the adsorption fits well with the Langmuir isotherm model and the pseudo-second-order kinetic model.
27246379	0	43	theme	sodium	61:66	arg1	cellulose					82:90	sodium carboxymethyl cellulose	61:90	sodium carboxymethyl cellulose	61:90	Removal of toxic indigo blue with integrated biomaterials of sodium carboxymethyl cellulose and chitosan.
27246379	5	44	theme	Langmuir	1109:1116	arg1	model					1127:1131	the Langmuir isotherm model	1105:1131	the Langmuir isotherm model	1105:1131	Finally, adsorption isotherms and adsorption kinetics are investigated and compared by using classic models, and the results indicate that the adsorption fits well with the Langmuir isotherm model and the pseudo-second-order kinetic model.
27246379	6	45	theme	integrated	1268:1277	arg1	biopolymers					1287:1297	integrated natural biopolymers	1268:1297	integrated natural biopolymers	1268:1297	It opens a new avenue for the effective and convenient treatment of textile wastewater with integrated natural biopolymers.
27246379	5	46	theme	pseudo-second-order	1141:1159	arg1	model					1169:1173	the pseudo-second-order kinetic model	1137:1173	the pseudo-second-order kinetic model	1137:1173	Finally, adsorption isotherms and adsorption kinetics are investigated and compared by using classic models, and the results indicate that the adsorption fits well with the Langmuir isotherm model and the pseudo-second-order kinetic model.
27246379	1	47	theme	obtained	212:219	arg1	CMC-CS-A					230:237	CMC-CS-A	230:237	CMC-CS-A	230:237	Sodium carboxymethyl cellulose (NaCMC) and chitosan (CS) are successfully combined via amidation, and the obtained product, CMC-CS-A, is used for the adsorption of toxic indigo blue (IB) for the first time.
27246379	1	47	theme	obtained	212:219	arg1	product					221:227	the obtained product	208:227	the obtained product	208:227	Sodium carboxymethyl cellulose (NaCMC) and chitosan (CS) are successfully combined via amidation, and the obtained product, CMC-CS-A, is used for the adsorption of toxic indigo blue (IB) for the first time.
27246379	6	48	theme	wastewater	1252:1261	arg1	treatment					1231:1239	the effective and convenient treatment	1202:1239	the effective and convenient treatment of textile wastewater with integrated natural biopolymers	1202:1297	It opens a new avenue for the effective and convenient treatment of textile wastewater with integrated natural biopolymers.
27246379	6	49	with	treatment	1231:1239	arg1	biopolymers					1287:1297	integrated natural biopolymers	1268:1297	integrated natural biopolymers	1268:1297	It opens a new avenue for the effective and convenient treatment of textile wastewater with integrated natural biopolymers.
27246379	3	50	theme	IB	773:774	arg1	removal					762:768	satisfactory removal	749:768	satisfactory removal of IB	749:774	The mass fraction of CS in the CMC-CS-A is also investigated to achieve the best adsorption of IB, and it reveals that satisfactory removal of IB can be achieved at the CMC-CS-A (50wt%).
26392244	9	0	from	substitution	1325:1336	arg1	β-cyclodextrins					1359:1373	sulphobutyl ether β-cyclodextrins	1341:1373	sulphobutyl ether β-cyclodextrins	1341:1373	The work is particularly concerned with producing controlled substitution in sulphobutyl ether β-cyclodextrins and novel compositions of highly substituted sulphoalkyl ether β-cyclodextrins are described.
26392244	8	1	theme	batch	1078:1082	arg1	mode					1094:1097	batch synthesis mode	1078:1097	batch synthesis mode	1078:1097	The activation reaction is carried out in batch synthesis mode and the sulphoalkylation reaction is carried out under continuous flow conditions resulting in a novel method for the synthesis of highly derivatised cyclodextrins.
26392244	9	2	theme	sulphoalkyl	1420:1430	arg1	β-cyclodextrins					1438:1452	highly substituted sulphoalkyl ether β-cyclodextrins	1401:1452	highly substituted sulphoalkyl ether β-cyclodextrins	1401:1452	The work is particularly concerned with producing controlled substitution in sulphobutyl ether β-cyclodextrins and novel compositions of highly substituted sulphoalkyl ether β-cyclodextrins are described.
26392244	1	3	theme	reagent	246:252	arg1	dissolution					254:264	initial reagent dissolution	238:264	initial reagent dissolution	238:264	Batch synthesis of sulphobutyl ether β-cyclodextrin (also known as SBE-β-CD or SBECD) is a process effectively divided into three main stages, i.e. initial reagent dissolution, a sulphoalkylation reaction and final reaction quenching.
26392244	1	3	theme	reagent	246:252	arg1	stages					225:230	three main stages	214:230	three main stages	214:230	Batch synthesis of sulphobutyl ether β-cyclodextrin (also known as SBE-β-CD or SBECD) is a process effectively divided into three main stages, i.e. initial reagent dissolution, a sulphoalkylation reaction and final reaction quenching.
26392244	5	4	theme	sulphobutyl	767:777	arg1	ether-cyclodextrins					779:797	sulphobutyl ether-cyclodextrins	767:797	sulphobutyl ether-cyclodextrins	767:797	The authors here present a Continuous Tank Reactor (CTR) method for preparing sulphobutyl ether-cyclodextrins.
26392244	7	5	with	cyclodextrin	956:967	arg1	sultone					988:994	an 1,4-butane sultone	974:994	an 1,4-butane sultone	974:994	The method then involves separately contacting the activated cyclodextrin with an 1,4-butane sultone to form sulphoalkyl ether-cyclodextrin.
26392244	2	6	theme	downstream	354:363	arg1	processing					365:374	processing	365:374	processing	365:374	This reaction is followed by downstream processing and purification, and ultimate isolation of the solid SBECD material.
26392244	5	7	theme	Reactor	732:738	arg1	method					746:751	a Continuous Tank Reactor (CTR) method	714:751	a Continuous Tank Reactor (CTR) method for preparing sulphobutyl ether-cyclodextrins	714:797	The authors here present a Continuous Tank Reactor (CTR) method for preparing sulphobutyl ether-cyclodextrins.
26392244	6	8	theme	contacting	827:836	arg1	cyclodextrin					838:849	first contacting cyclodextrin	821:849	first contacting cyclodextrin with a base to form activated cyclodextrin	821:892	The method comprises first contacting cyclodextrin with a base to form activated cyclodextrin.
26392244	1	9	dep	known	148:152	arg1	also					143:146	also	143:146	also	143:146	Batch synthesis of sulphobutyl ether β-cyclodextrin (also known as SBE-β-CD or SBECD) is a process effectively divided into three main stages, i.e. initial reagent dissolution, a sulphoalkylation reaction and final reaction quenching.
26392244	8	10	theme	synthesis	1084:1092	arg1	mode					1094:1097	batch synthesis mode	1078:1097	batch synthesis mode	1078:1097	The activation reaction is carried out in batch synthesis mode and the sulphoalkylation reaction is carried out under continuous flow conditions resulting in a novel method for the synthesis of highly derivatised cyclodextrins.
26392244	6	11	theme	first	821:825	arg1	cyclodextrin					838:849	first contacting cyclodextrin	821:849	first contacting cyclodextrin with a base to form activated cyclodextrin	821:892	The method comprises first contacting cyclodextrin with a base to form activated cyclodextrin.
26392244	3	12	theme	substituted	544:554	arg1	SBECD					556:560	lower substituted SBECD	538:560	lower substituted SBECD	538:560	However, a feature associated with using this synthetic method is that a high proportion of lower substituted SBECD is observed.
26392244	8	13	theme	derivatised	1237:1247	arg1	cyclodextrins					1249:1261	highly derivatised cyclodextrins	1230:1261	highly derivatised cyclodextrins	1230:1261	The activation reaction is carried out in batch synthesis mode and the sulphoalkylation reaction is carried out under continuous flow conditions resulting in a novel method for the synthesis of highly derivatised cyclodextrins.
26392244	5	14	theme	CTR	741:743	arg1	method					746:751	a Continuous Tank Reactor (CTR) method	714:751	a Continuous Tank Reactor (CTR) method for preparing sulphobutyl ether-cyclodextrins	714:797	The authors here present a Continuous Tank Reactor (CTR) method for preparing sulphobutyl ether-cyclodextrins.
26392244	4	15	theme	improved	615:622	arg1	method					634:639	an improved synthetic method	612:639	an improved synthetic method for producing higher substituted cyclodextrins	612:686	There is therefore a need to provide an improved synthetic method for producing higher substituted cyclodextrins.
26392244	1	16	theme	Batch	90:94	arg1	process					181:187	a process	179:187	a process effectively divided into three main stages, i.e. initial reagent dissolution, a sulphoalkylation reaction and final reaction quenching	179:322	Batch synthesis of sulphobutyl ether β-cyclodextrin (also known as SBE-β-CD or SBECD) is a process effectively divided into three main stages, i.e. initial reagent dissolution, a sulphoalkylation reaction and final reaction quenching.
26392244	1	16	theme	Batch	90:94	arg1	synthesis					96:104	Batch synthesis	90:104	Batch synthesis of sulphobutyl ether β-cyclodextrin (also known as SBE-β-CD or SBECD)	90:174	Batch synthesis of sulphobutyl ether β-cyclodextrin (also known as SBE-β-CD or SBECD) is a process effectively divided into three main stages, i.e. initial reagent dissolution, a sulphoalkylation reaction and final reaction quenching.
26392244	8	17	theme	cyclodextrins	1249:1261	arg1	synthesis					1217:1225	the synthesis	1213:1225	the synthesis of highly derivatised cyclodextrins	1213:1261	The activation reaction is carried out in batch synthesis mode and the sulphoalkylation reaction is carried out under continuous flow conditions resulting in a novel method for the synthesis of highly derivatised cyclodextrins.
26392244	4	18	theme	higher	655:660	arg1	cyclodextrins					674:686	higher substituted cyclodextrins	655:686	higher substituted cyclodextrins	655:686	There is therefore a need to provide an improved synthetic method for producing higher substituted cyclodextrins.
26392244	9	19	theme	substituted	1408:1418	arg1	β-cyclodextrins					1438:1452	highly substituted sulphoalkyl ether β-cyclodextrins	1401:1452	highly substituted sulphoalkyl ether β-cyclodextrins	1401:1452	The work is particularly concerned with producing controlled substitution in sulphobutyl ether β-cyclodextrins and novel compositions of highly substituted sulphoalkyl ether β-cyclodextrins are described.
26392244	2	20	theme	ultimate	398:405	arg1	isolation					407:415	ultimate isolation	398:415	ultimate isolation of the solid SBECD material	398:443	This reaction is followed by downstream processing and purification, and ultimate isolation of the solid SBECD material.
26392244	0	21	theme	tank	11:14	arg1	synthesis					24:32	Continuous tank reactor synthesis	0:32	Continuous tank reactor synthesis of highly substituted sulphobutylether β-cyclodextrins.	0:88	Continuous tank reactor synthesis of highly substituted sulphobutylether β-cyclodextrins.
26392244	8	22	theme	novel	1196:1200	arg1	method					1202:1207	a novel method	1194:1207	a novel method for the synthesis of highly derivatised cyclodextrins	1194:1261	The activation reaction is carried out in batch synthesis mode and the sulphoalkylation reaction is carried out under continuous flow conditions resulting in a novel method for the synthesis of highly derivatised cyclodextrins.
26392244	7	23	theme	1,4-butane	977:986	arg1	sultone					988:994	an 1,4-butane sultone	974:994	an 1,4-butane sultone	974:994	The method then involves separately contacting the activated cyclodextrin with an 1,4-butane sultone to form sulphoalkyl ether-cyclodextrin.
26392244	0	24	theme	Continuous	0:9	arg1	synthesis					24:32	Continuous tank reactor synthesis	0:32	Continuous tank reactor synthesis of highly substituted sulphobutylether β-cyclodextrins.	0:88	Continuous tank reactor synthesis of highly substituted sulphobutylether β-cyclodextrins.
26392244	1	25	theme	sulphoalkylation	269:284	arg1	stages					225:230	three main stages	214:230	three main stages	214:230	Batch synthesis of sulphobutyl ether β-cyclodextrin (also known as SBE-β-CD or SBECD) is a process effectively divided into three main stages, i.e. initial reagent dissolution, a sulphoalkylation reaction and final reaction quenching.
26392244	1	25	theme	sulphoalkylation	269:284	arg1	reaction					286:293	a sulphoalkylation reaction	267:293	a sulphoalkylation reaction	267:293	Batch synthesis of sulphobutyl ether β-cyclodextrin (also known as SBE-β-CD or SBECD) is a process effectively divided into three main stages, i.e. initial reagent dissolution, a sulphoalkylation reaction and final reaction quenching.
26392244	8	26	theme	sulphoalkylation	1107:1122	arg1	reaction					1124:1131	the sulphoalkylation reaction	1103:1131	the sulphoalkylation reaction	1103:1131	The activation reaction is carried out in batch synthesis mode and the sulphoalkylation reaction is carried out under continuous flow conditions resulting in a novel method for the synthesis of highly derivatised cyclodextrins.
26392244	1	27	theme	sulphobutyl	109:119	arg1	β-cyclodextrin					127:140	sulphobutyl ether β-cyclodextrin	109:140	sulphobutyl ether β-cyclodextrin (also known as SBE-β-CD or SBECD)	109:174	Batch synthesis of sulphobutyl ether β-cyclodextrin (also known as SBE-β-CD or SBECD) is a process effectively divided into three main stages, i.e. initial reagent dissolution, a sulphoalkylation reaction and final reaction quenching.
26392244	4	28	theme	substituted	662:672	arg1	cyclodextrins					674:686	higher substituted cyclodextrins	655:686	higher substituted cyclodextrins	655:686	There is therefore a need to provide an improved synthetic method for producing higher substituted cyclodextrins.
26392244	6	29	with	cyclodextrin	838:849	arg1	base					858:861	a base	856:861	a base to form activated cyclodextrin	856:892	The method comprises first contacting cyclodextrin with a base to form activated cyclodextrin.
26392244	9	30	theme	β-cyclodextrins	1438:1452	arg1	compositions					1385:1396	novel compositions	1379:1396	novel compositions of highly substituted sulphoalkyl ether β-cyclodextrins	1379:1452	The work is particularly concerned with producing controlled substitution in sulphobutyl ether β-cyclodextrins and novel compositions of highly substituted sulphoalkyl ether β-cyclodextrins are described.
26392244	7	31	dep	involves	911:918	arg1	contacting					931:940	contacting	931:940	involves separately contacting the activated cyclodextrin with an 1,4-butane sultone to form sulphoalkyl ether-cyclodextrin	911:1033	The method then involves separately contacting the activated cyclodextrin with an 1,4-butane sultone to form sulphoalkyl ether-cyclodextrin.
26392244	1	32	theme	ether	121:125	arg1	β-cyclodextrin					127:140	sulphobutyl ether β-cyclodextrin	109:140	sulphobutyl ether β-cyclodextrin (also known as SBE-β-CD or SBECD)	109:174	Batch synthesis of sulphobutyl ether β-cyclodextrin (also known as SBE-β-CD or SBECD) is a process effectively divided into three main stages, i.e. initial reagent dissolution, a sulphoalkylation reaction and final reaction quenching.
26392244	2	33	theme	SBECD	430:434	arg1	material					436:443	the solid SBECD material	420:443	the solid SBECD material	420:443	This reaction is followed by downstream processing and purification, and ultimate isolation of the solid SBECD material.
26392244	2	34	theme	material	436:443	arg1	processing					365:374	processing	365:374	processing	365:374	This reaction is followed by downstream processing and purification, and ultimate isolation of the solid SBECD material.
26392244	2	34	theme	material	436:443	arg1	isolation					407:415	ultimate isolation	398:415	ultimate isolation of the solid SBECD material	398:443	This reaction is followed by downstream processing and purification, and ultimate isolation of the solid SBECD material.
26392244	2	34	theme	material	436:443	arg1	purification					380:391	purification	380:391	purification	380:391	This reaction is followed by downstream processing and purification, and ultimate isolation of the solid SBECD material.
26392244	9	35	theme	ether	1432:1436	arg1	β-cyclodextrins					1438:1452	highly substituted sulphoalkyl ether β-cyclodextrins	1401:1452	highly substituted sulphoalkyl ether β-cyclodextrins	1401:1452	The work is particularly concerned with producing controlled substitution in sulphobutyl ether β-cyclodextrins and novel compositions of highly substituted sulphoalkyl ether β-cyclodextrins are described.
26392244	1	36	theme	β-cyclodextrin	127:140	arg1	process					181:187	a process	179:187	a process effectively divided into three main stages, i.e. initial reagent dissolution, a sulphoalkylation reaction and final reaction quenching	179:322	Batch synthesis of sulphobutyl ether β-cyclodextrin (also known as SBE-β-CD or SBECD) is a process effectively divided into three main stages, i.e. initial reagent dissolution, a sulphoalkylation reaction and final reaction quenching.
26392244	1	36	theme	β-cyclodextrin	127:140	arg1	synthesis					96:104	Batch synthesis	90:104	Batch synthesis of sulphobutyl ether β-cyclodextrin (also known as SBE-β-CD or SBECD)	90:174	Batch synthesis of sulphobutyl ether β-cyclodextrin (also known as SBE-β-CD or SBECD) is a process effectively divided into three main stages, i.e. initial reagent dissolution, a sulphoalkylation reaction and final reaction quenching.
26392244	2	37	theme	solid	424:428	arg1	material					436:443	the solid SBECD material	420:443	the solid SBECD material	420:443	This reaction is followed by downstream processing and purification, and ultimate isolation of the solid SBECD material.
26392244	1	38	theme	final	299:303	arg1	quenching					314:322	final reaction quenching	299:322	final reaction quenching	299:322	Batch synthesis of sulphobutyl ether β-cyclodextrin (also known as SBE-β-CD or SBECD) is a process effectively divided into three main stages, i.e. initial reagent dissolution, a sulphoalkylation reaction and final reaction quenching.
26392244	1	38	theme	final	299:303	arg1	stages					225:230	three main stages	214:230	three main stages	214:230	Batch synthesis of sulphobutyl ether β-cyclodextrin (also known as SBE-β-CD or SBECD) is a process effectively divided into three main stages, i.e. initial reagent dissolution, a sulphoalkylation reaction and final reaction quenching.
26392244	5	39	theme	Continuous	716:725	arg1	method					746:751	a Continuous Tank Reactor (CTR) method	714:751	a Continuous Tank Reactor (CTR) method for preparing sulphobutyl ether-cyclodextrins	714:797	The authors here present a Continuous Tank Reactor (CTR) method for preparing sulphobutyl ether-cyclodextrins.
26392244	1	40	theme	reaction	305:312	arg1	quenching					314:322	final reaction quenching	299:322	final reaction quenching	299:322	Batch synthesis of sulphobutyl ether β-cyclodextrin (also known as SBE-β-CD or SBECD) is a process effectively divided into three main stages, i.e. initial reagent dissolution, a sulphoalkylation reaction and final reaction quenching.
26392244	1	40	theme	reaction	305:312	arg1	stages					225:230	three main stages	214:230	three main stages	214:230	Batch synthesis of sulphobutyl ether β-cyclodextrin (also known as SBE-β-CD or SBECD) is a process effectively divided into three main stages, i.e. initial reagent dissolution, a sulphoalkylation reaction and final reaction quenching.
26392244	5	41	theme	Tank	727:730	arg1	method					746:751	a Continuous Tank Reactor (CTR) method	714:751	a Continuous Tank Reactor (CTR) method for preparing sulphobutyl ether-cyclodextrins	714:797	The authors here present a Continuous Tank Reactor (CTR) method for preparing sulphobutyl ether-cyclodextrins.
26392244	1	42	dep	stages	225:230	arg1	quenching					314:322	final reaction quenching	299:322	final reaction quenching	299:322	Batch synthesis of sulphobutyl ether β-cyclodextrin (also known as SBE-β-CD or SBECD) is a process effectively divided into three main stages, i.e. initial reagent dissolution, a sulphoalkylation reaction and final reaction quenching.
26392244	1	42	dep	stages	225:230	arg1	stages					225:230	three main stages	214:230	three main stages	214:230	Batch synthesis of sulphobutyl ether β-cyclodextrin (also known as SBE-β-CD or SBECD) is a process effectively divided into three main stages, i.e. initial reagent dissolution, a sulphoalkylation reaction and final reaction quenching.
26392244	1	42	dep	stages	225:230	arg1	reaction					286:293	a sulphoalkylation reaction	267:293	a sulphoalkylation reaction	267:293	Batch synthesis of sulphobutyl ether β-cyclodextrin (also known as SBE-β-CD or SBECD) is a process effectively divided into three main stages, i.e. initial reagent dissolution, a sulphoalkylation reaction and final reaction quenching.
26392244	1	42	dep	stages	225:230	arg1	dissolution					254:264	initial reagent dissolution	238:264	initial reagent dissolution	238:264	Batch synthesis of sulphobutyl ether β-cyclodextrin (also known as SBE-β-CD or SBECD) is a process effectively divided into three main stages, i.e. initial reagent dissolution, a sulphoalkylation reaction and final reaction quenching.
26392244	1	43	theme	main	220:223	arg1	quenching					314:322	final reaction quenching	299:322	final reaction quenching	299:322	Batch synthesis of sulphobutyl ether β-cyclodextrin (also known as SBE-β-CD or SBECD) is a process effectively divided into three main stages, i.e. initial reagent dissolution, a sulphoalkylation reaction and final reaction quenching.
26392244	1	43	theme	main	220:223	arg1	stages					225:230	three main stages	214:230	three main stages	214:230	Batch synthesis of sulphobutyl ether β-cyclodextrin (also known as SBE-β-CD or SBECD) is a process effectively divided into three main stages, i.e. initial reagent dissolution, a sulphoalkylation reaction and final reaction quenching.
26392244	1	43	theme	main	220:223	arg1	reaction					286:293	a sulphoalkylation reaction	267:293	a sulphoalkylation reaction	267:293	Batch synthesis of sulphobutyl ether β-cyclodextrin (also known as SBE-β-CD or SBECD) is a process effectively divided into three main stages, i.e. initial reagent dissolution, a sulphoalkylation reaction and final reaction quenching.
26392244	1	43	theme	main	220:223	arg1	dissolution					254:264	initial reagent dissolution	238:264	initial reagent dissolution	238:264	Batch synthesis of sulphobutyl ether β-cyclodextrin (also known as SBE-β-CD or SBECD) is a process effectively divided into three main stages, i.e. initial reagent dissolution, a sulphoalkylation reaction and final reaction quenching.
26392244	0	44	theme	sulphobutylether	56:71	arg1	β-cyclodextrins					73:87	highly substituted sulphobutylether β-cyclodextrins	37:87	highly substituted sulphobutylether β-cyclodextrins	37:87	Continuous tank reactor synthesis of highly substituted sulphobutylether β-cyclodextrins.
26392244	8	45	theme	flow	1165:1168	arg1	conditions					1170:1179	continuous flow conditions	1154:1179	continuous flow conditions resulting in a novel method for the synthesis of highly derivatised cyclodextrins	1154:1261	The activation reaction is carried out in batch synthesis mode and the sulphoalkylation reaction is carried out under continuous flow conditions resulting in a novel method for the synthesis of highly derivatised cyclodextrins.
26392244	9	46	theme	controlled	1314:1323	arg1	substitution					1325:1336	controlled substitution	1314:1336	controlled substitution in sulphobutyl ether β-cyclodextrins	1314:1373	The work is particularly concerned with producing controlled substitution in sulphobutyl ether β-cyclodextrins and novel compositions of highly substituted sulphoalkyl ether β-cyclodextrins are described.
26392244	3	47	theme	synthetic	492:500	arg1	method					502:507	this synthetic method	487:507	this synthetic method	487:507	However, a feature associated with using this synthetic method is that a high proportion of lower substituted SBECD is observed.
26392244	0	48	theme	substituted	44:54	arg1	β-cyclodextrins					73:87	highly substituted sulphobutylether β-cyclodextrins	37:87	highly substituted sulphobutylether β-cyclodextrins	37:87	Continuous tank reactor synthesis of highly substituted sulphobutylether β-cyclodextrins.
26392244	8	49	theme	continuous	1154:1163	arg1	conditions					1170:1179	continuous flow conditions	1154:1179	continuous flow conditions resulting in a novel method for the synthesis of highly derivatised cyclodextrins	1154:1261	The activation reaction is carried out in batch synthesis mode and the sulphoalkylation reaction is carried out under continuous flow conditions resulting in a novel method for the synthesis of highly derivatised cyclodextrins.
26392244	6	50	theme	activated	871:879	arg1	cyclodextrin					881:892	activated cyclodextrin	871:892	activated cyclodextrin	871:892	The method comprises first contacting cyclodextrin with a base to form activated cyclodextrin.
26392244	8	51	theme	activation	1040:1049	arg1	reaction					1051:1058	The activation reaction	1036:1058	The activation reaction	1036:1058	The activation reaction is carried out in batch synthesis mode and the sulphoalkylation reaction is carried out under continuous flow conditions resulting in a novel method for the synthesis of highly derivatised cyclodextrins.
26392244	7	52	theme	sulphoalkyl	1004:1014	arg1	ether-cyclodextrin					1016:1033	sulphoalkyl ether-cyclodextrin	1004:1033	sulphoalkyl ether-cyclodextrin	1004:1033	The method then involves separately contacting the activated cyclodextrin with an 1,4-butane sultone to form sulphoalkyl ether-cyclodextrin.
26392244	3	53	theme	SBECD	556:560	arg1	proportion					524:533	a high proportion	517:533	a high proportion of lower substituted SBECD	517:560	However, a feature associated with using this synthetic method is that a high proportion of lower substituted SBECD is observed.
26392244	9	54	theme	sulphobutyl	1341:1351	arg1	β-cyclodextrins					1359:1373	sulphobutyl ether β-cyclodextrins	1341:1373	sulphobutyl ether β-cyclodextrins	1341:1373	The work is particularly concerned with producing controlled substitution in sulphobutyl ether β-cyclodextrins and novel compositions of highly substituted sulphoalkyl ether β-cyclodextrins are described.
26392244	3	55	theme	high	519:522	arg1	proportion					524:533	a high proportion	517:533	a high proportion of lower substituted SBECD	517:560	However, a feature associated with using this synthetic method is that a high proportion of lower substituted SBECD is observed.
26392244	9	56	theme	ether	1353:1357	arg1	β-cyclodextrins					1359:1373	sulphobutyl ether β-cyclodextrins	1341:1373	sulphobutyl ether β-cyclodextrins	1341:1373	The work is particularly concerned with producing controlled substitution in sulphobutyl ether β-cyclodextrins and novel compositions of highly substituted sulphoalkyl ether β-cyclodextrins are described.
26392244	4	57	theme	synthetic	624:632	arg1	method					634:639	an improved synthetic method	612:639	an improved synthetic method for producing higher substituted cyclodextrins	612:686	There is therefore a need to provide an improved synthetic method for producing higher substituted cyclodextrins.
26392244	7	58	theme	activated	946:954	arg1	cyclodextrin					956:967	the activated cyclodextrin	942:967	the activated cyclodextrin with an 1,4-butane sultone to form sulphoalkyl ether-cyclodextrin	942:1033	The method then involves separately contacting the activated cyclodextrin with an 1,4-butane sultone to form sulphoalkyl ether-cyclodextrin.
26392244	0	59	theme	β-cyclodextrins	73:87	arg1	synthesis					24:32	Continuous tank reactor synthesis	0:32	Continuous tank reactor synthesis of highly substituted sulphobutylether β-cyclodextrins.	0:88	Continuous tank reactor synthesis of highly substituted sulphobutylether β-cyclodextrins.
26392244	9	60	theme	novel	1379:1383	arg1	compositions					1385:1396	novel compositions	1379:1396	novel compositions of highly substituted sulphoalkyl ether β-cyclodextrins	1379:1452	The work is particularly concerned with producing controlled substitution in sulphobutyl ether β-cyclodextrins and novel compositions of highly substituted sulphoalkyl ether β-cyclodextrins are described.
26392244	0	61	theme	reactor	16:22	arg1	synthesis					24:32	Continuous tank reactor synthesis	0:32	Continuous tank reactor synthesis of highly substituted sulphobutylether β-cyclodextrins.	0:88	Continuous tank reactor synthesis of highly substituted sulphobutylether β-cyclodextrins.
26392244	1	62	dep	β-cyclodextrin	127:140	arg1	known					148:152	known	148:152	known as SBE-β-CD or SBECD	148:173	Batch synthesis of sulphobutyl ether β-cyclodextrin (also known as SBE-β-CD or SBECD) is a process effectively divided into three main stages, i.e. initial reagent dissolution, a sulphoalkylation reaction and final reaction quenching.
26392244	1	63	theme	initial	238:244	arg1	dissolution					254:264	initial reagent dissolution	238:264	initial reagent dissolution	238:264	Batch synthesis of sulphobutyl ether β-cyclodextrin (also known as SBE-β-CD or SBECD) is a process effectively divided into three main stages, i.e. initial reagent dissolution, a sulphoalkylation reaction and final reaction quenching.
26392244	1	63	theme	initial	238:244	arg1	stages					225:230	three main stages	214:230	three main stages	214:230	Batch synthesis of sulphobutyl ether β-cyclodextrin (also known as SBE-β-CD or SBECD) is a process effectively divided into three main stages, i.e. initial reagent dissolution, a sulphoalkylation reaction and final reaction quenching.
28551436	8	0	theme	decreased	1296:1304	arg1	expression					1306:1315	decreased expression	1296:1315	decreased expression of ECM markers- Collagen type II and Glycosaminoglycans	1296:1371	Although there was an increase in the percentage of viable encapsulated cells which also maintained the spherical phenotype in the less stiff gels, decreased expression of ECM markers- Collagen type II and Glycosaminoglycans was observed compared to the stiffer gels.
28551436	7	1	from	decrease	1122:1129	arg1	time					1142:1145	gelling time	1134:1145	gelling time	1134:1145	The results of the study show that as the ratio of hyaluronic acid dialdehyde component was increased, the stiffness of the gels increased from 130.78±19.83kPa to 181.47±19.77kPa which was also evidenced from the decrease in gelling time.
28551436	8	2	from	increase	1170:1177	arg1	percentage					1186:1195	the percentage	1182:1195	the percentage of viable encapsulated cells	1182:1224	Although there was an increase in the percentage of viable encapsulated cells which also maintained the spherical phenotype in the less stiff gels, decreased expression of ECM markers- Collagen type II and Glycosaminoglycans was observed compared to the stiffer gels.
28551436	6	3	theme	ECM	812:814	arg1	production					816:825	ECM production	812:825	ECM production	812:825	The viability and ECM production of encapsulated chondrocytes were assessed at 7day, 14day and 28day post culture.
28551436	7	4	theme	gels	1033:1036	arg1	stiffness					1016:1024	the stiffness	1012:1024	the stiffness of the gels	1012:1036	The results of the study show that as the ratio of hyaluronic acid dialdehyde component was increased, the stiffness of the gels increased from 130.78±19.83kPa to 181.47±19.77kPa which was also evidenced from the decrease in gelling time.
28551436	8	5	theme	stiffer	1402:1408	arg1	gels					1410:1413	the stiffer gels	1398:1413	the stiffer gels	1398:1413	Although there was an increase in the percentage of viable encapsulated cells which also maintained the spherical phenotype in the less stiff gels, decreased expression of ECM markers- Collagen type II and Glycosaminoglycans was observed compared to the stiffer gels.
28551436	8	6	theme	ECM	1320:1322	arg1	Collagen					1333:1340	ECM markers- Collagen type II and Glycosaminoglycans	1320:1371	ECM markers- Collagen type II and Glycosaminoglycans	1320:1371	Although there was an increase in the percentage of viable encapsulated cells which also maintained the spherical phenotype in the less stiff gels, decreased expression of ECM markers- Collagen type II and Glycosaminoglycans was observed compared to the stiffer gels.
28551436	4	7	theme	AFM	662:664	arg1	analysis					666:673	force spectroscopy AFM analysis	643:673	force spectroscopy AFM analysis	643:673	The stiffness of the gels was evaluated from the force displacement curves using force spectroscopy AFM analysis.
28551436	8	8	from	phenotype	1262:1270	arg1	gels					1290:1293	the less stiff gels	1275:1293	the less stiff gels	1275:1293	Although there was an increase in the percentage of viable encapsulated cells which also maintained the spherical phenotype in the less stiff gels, decreased expression of ECM markers- Collagen type II and Glycosaminoglycans was observed compared to the stiffer gels.
28551436	8	9	theme	markers-	1324:1331	arg1	Collagen					1333:1340	ECM markers- Collagen type II and Glycosaminoglycans	1320:1371	ECM markers- Collagen type II and Glycosaminoglycans	1320:1371	Although there was an increase in the percentage of viable encapsulated cells which also maintained the spherical phenotype in the less stiff gels, decreased expression of ECM markers- Collagen type II and Glycosaminoglycans was observed compared to the stiffer gels.
28551436	9	10	theme	production	1561:1570	arg1	maintenance					1517:1527	maintenance	1517:1527	maintenance of phenotypic integrity and ECM production	1517:1570	These findings indicate that gel stiffness strongly impacts the chondrocyte microenvironment both in maintenance of phenotypic integrity and ECM production.
28551436	9	11	theme	gel	1445:1447	arg1	stiffness					1449:1457	gel stiffness	1445:1457	gel stiffness	1445:1457	These findings indicate that gel stiffness strongly impacts the chondrocyte microenvironment both in maintenance of phenotypic integrity and ECM production.
28551436	4	12	theme	gels	583:586	arg1	stiffness					566:574	The stiffness	562:574	The stiffness of the gels	562:586	The stiffness of the gels was evaluated from the force displacement curves using force spectroscopy AFM analysis.
28551436	0	13	theme	chondrocytes	113:124	arg1	viability					76:84	viability	76:84	viability	76:84	Effect of stiffness of chitosan-hyaluronic acid dialdehyde hydrogels on the viability and growth of encapsulated chondrocytes.
28551436	0	13	theme	chondrocytes	113:124	arg1	growth					90:95	growth	90:95	growth	90:95	Effect of stiffness of chitosan-hyaluronic acid dialdehyde hydrogels on the viability and growth of encapsulated chondrocytes.
28551436	4	14	theme	displacement	617:628	arg1	curves					630:635	the force displacement curves	607:635	the force displacement curves using force spectroscopy AFM analysis	607:673	The stiffness of the gels was evaluated from the force displacement curves using force spectroscopy AFM analysis.
28551436	2	15	theme	compositions	295:306	arg1	effect					267:272	the effect	263:272	the effect of varying stiffness compositions of a two-component injectable hydrogel based on chitosan (CH) and oxidized hyaluronic acid (HDA) on the growth and functionality of encapsulated chondrocytes	263:464	This work aimed to study the effect of varying stiffness compositions of a two-component injectable hydrogel based on chitosan (CH) and oxidized hyaluronic acid (HDA) on the growth and functionality of encapsulated chondrocytes.
28551436	1	16	theme	phenotypic	179:188	arg1	characteristics					205:219	the phenotypic and functional characteristics	175:219	the phenotypic and functional characteristics of chondrocytes	175:235	Substrate elasticity or stiffness can influence the phenotypic and functional characteristics of chondrocytes.
28551436	0	17	theme	encapsulated	100:111	arg1	chondrocytes					113:124	encapsulated chondrocytes	100:124	encapsulated chondrocytes	100:124	Effect of stiffness of chitosan-hyaluronic acid dialdehyde hydrogels on the viability and growth of encapsulated chondrocytes.
28551436	5	18	used	used	760:763	arg2	cells					729:733	the cells	725:733	the cells from Passage 2 to 4	725:753	Rabbit articular chondrocytes were harvested and the cells from Passage 2 to 4 were used for the encapsulation study.
28551436	2	19	theme	stiffness	285:293	arg1	compositions					295:306	varying stiffness compositions	277:306	varying stiffness compositions	277:306	This work aimed to study the effect of varying stiffness compositions of a two-component injectable hydrogel based on chitosan (CH) and oxidized hyaluronic acid (HDA) on the growth and functionality of encapsulated chondrocytes.
28551436	4	20	theme	spectroscopy	649:660	arg1	analysis					666:673	force spectroscopy AFM analysis	643:673	force spectroscopy AFM analysis	643:673	The stiffness of the gels was evaluated from the force displacement curves using force spectroscopy AFM analysis.
28551436	2	21	theme	chondrocytes	453:464	arg1	functionality					423:435	functionality	423:435	functionality	423:435	This work aimed to study the effect of varying stiffness compositions of a two-component injectable hydrogel based on chitosan (CH) and oxidized hyaluronic acid (HDA) on the growth and functionality of encapsulated chondrocytes.
28551436	2	21	theme	chondrocytes	453:464	arg1	growth					412:417	growth	412:417	growth	412:417	This work aimed to study the effect of varying stiffness compositions of a two-component injectable hydrogel based on chitosan (CH) and oxidized hyaluronic acid (HDA) on the growth and functionality of encapsulated chondrocytes.
28551436	7	22	theme	study	928:932	arg1	results					913:919	The results	909:919	The results of the study	909:932	The results of the study show that as the ratio of hyaluronic acid dialdehyde component was increased, the stiffness of the gels increased from 130.78±19.83kPa to 181.47±19.77kPa which was also evidenced from the decrease in gelling time.
28551436	3	23	dep	prepared	506:513	arg1	10:1,10:3					516:524	10:1,10:3	516:524	10:1,10:3	516:524	Three different ratios of the gel were prepared (10:1,10:3 and 10:5 CH-HDA) and characterized.
28551436	3	23	dep	prepared	506:513	arg1	CH-HDA					535:540	10:5 CH-HDA	530:540	10:5 CH-HDA	530:540	Three different ratios of the gel were prepared (10:1,10:3 and 10:5 CH-HDA) and characterized.
28551436	1	24	theme	functional	194:203	arg1	characteristics					205:219	the phenotypic and functional characteristics	175:219	the phenotypic and functional characteristics of chondrocytes	175:235	Substrate elasticity or stiffness can influence the phenotypic and functional characteristics of chondrocytes.
28551436	2	25	theme	encapsulated	440:451	arg1	chondrocytes					453:464	encapsulated chondrocytes	440:464	encapsulated chondrocytes	440:464	This work aimed to study the effect of varying stiffness compositions of a two-component injectable hydrogel based on chitosan (CH) and oxidized hyaluronic acid (HDA) on the growth and functionality of encapsulated chondrocytes.
28551436	7	26	theme	gelling	1134:1140	arg1	time					1142:1145	gelling time	1134:1145	gelling time	1134:1145	The results of the study show that as the ratio of hyaluronic acid dialdehyde component was increased, the stiffness of the gels increased from 130.78±19.83kPa to 181.47±19.77kPa which was also evidenced from the decrease in gelling time.
28551436	8	27	theme	cells	1220:1224	arg1	percentage					1186:1195	the percentage	1182:1195	the percentage of viable encapsulated cells	1182:1224	Although there was an increase in the percentage of viable encapsulated cells which also maintained the spherical phenotype in the less stiff gels, decreased expression of ECM markers- Collagen type II and Glycosaminoglycans was observed compared to the stiffer gels.
28551436	0	28	dep	viability	76:84	arg1	the					72:74	the	72:74	the	72:74	Effect of stiffness of chitosan-hyaluronic acid dialdehyde hydrogels on the viability and growth of encapsulated chondrocytes.
28551436	6	29	dep	viability	798:806	arg1	The					794:796	The	794:796	The	794:796	The viability and ECM production of encapsulated chondrocytes were assessed at 7day, 14day and 28day post culture.
28551436	8	30	dep	Collagen	1333:1340	arg1	type					1342:1345	type II	1342:1348	type II	1342:1348	Although there was an increase in the percentage of viable encapsulated cells which also maintained the spherical phenotype in the less stiff gels, decreased expression of ECM markers- Collagen type II and Glycosaminoglycans was observed compared to the stiffer gels.
28551436	8	30	dep	Collagen	1333:1340	arg1	Glycosaminoglycans					1354:1371	Glycosaminoglycans	1354:1371	Glycosaminoglycans	1354:1371	Although there was an increase in the percentage of viable encapsulated cells which also maintained the spherical phenotype in the less stiff gels, decreased expression of ECM markers- Collagen type II and Glycosaminoglycans was observed compared to the stiffer gels.
28551436	2	31	theme	injectable	327:336	arg1	hydrogel					338:345	a two-component injectable hydrogel	311:345	a two-component injectable hydrogel based on chitosan (CH) and oxidized hyaluronic acid (HDA) on the growth and functionality of encapsulated chondrocytes	311:464	This work aimed to study the effect of varying stiffness compositions of a two-component injectable hydrogel based on chitosan (CH) and oxidized hyaluronic acid (HDA) on the growth and functionality of encapsulated chondrocytes.
28551436	3	32	theme	different	473:481	arg1	ratios					483:488	Three different ratios	467:488	Three different ratios of the gel	467:499	Three different ratios of the gel were prepared (10:1,10:3 and 10:5 CH-HDA) and characterized.
28551436	2	33	theme	hydrogel	338:345	arg1	effect					267:272	the effect	263:272	the effect of varying stiffness compositions of a two-component injectable hydrogel based on chitosan (CH) and oxidized hyaluronic acid (HDA) on the growth and functionality of encapsulated chondrocytes	263:464	This work aimed to study the effect of varying stiffness compositions of a two-component injectable hydrogel based on chitosan (CH) and oxidized hyaluronic acid (HDA) on the growth and functionality of encapsulated chondrocytes.
28551436	3	34	theme	10:5	530:533	arg1	CH-HDA					535:540	10:5 CH-HDA	530:540	10:5 CH-HDA	530:540	Three different ratios of the gel were prepared (10:1,10:3 and 10:5 CH-HDA) and characterized.
28551436	2	35	theme	two-component	313:325	arg1	hydrogel					338:345	a two-component injectable hydrogel	311:345	a two-component injectable hydrogel based on chitosan (CH) and oxidized hyaluronic acid (HDA) on the growth and functionality of encapsulated chondrocytes	311:464	This work aimed to study the effect of varying stiffness compositions of a two-component injectable hydrogel based on chitosan (CH) and oxidized hyaluronic acid (HDA) on the growth and functionality of encapsulated chondrocytes.
28551436	9	36	theme	phenotypic	1532:1541	arg1	integrity					1543:1551	phenotypic integrity	1532:1551	phenotypic integrity	1532:1551	These findings indicate that gel stiffness strongly impacts the chondrocyte microenvironment both in maintenance of phenotypic integrity and ECM production.
28551436	1	37	theme	chondrocytes	224:235	arg1	characteristics					205:219	the phenotypic and functional characteristics	175:219	the phenotypic and functional characteristics of chondrocytes	175:235	Substrate elasticity or stiffness can influence the phenotypic and functional characteristics of chondrocytes.
28551436	0	38	theme	acid	43:46	arg1	dialdehyde					48:57	chitosan-hyaluronic acid dialdehyde	23:57	chitosan-hyaluronic acid dialdehyde	23:57	Effect of stiffness of chitosan-hyaluronic acid dialdehyde hydrogels on the viability and growth of encapsulated chondrocytes.
28551436	9	39	theme	chondrocyte	1480:1490	arg1	microenvironment					1492:1507	the chondrocyte microenvironment	1476:1507	the chondrocyte microenvironment	1476:1507	These findings indicate that gel stiffness strongly impacts the chondrocyte microenvironment both in maintenance of phenotypic integrity and ECM production.
28551436	4	40	theme	force	643:647	arg1	analysis					666:673	force spectroscopy AFM analysis	643:673	force spectroscopy AFM analysis	643:673	The stiffness of the gels was evaluated from the force displacement curves using force spectroscopy AFM analysis.
28551436	0	41	theme	chitosan-hyaluronic	23:41	arg1	dialdehyde					48:57	chitosan-hyaluronic acid dialdehyde	23:57	chitosan-hyaluronic acid dialdehyde	23:57	Effect of stiffness of chitosan-hyaluronic acid dialdehyde hydrogels on the viability and growth of encapsulated chondrocytes.
28551436	5	42	theme	encapsulation	773:785	arg1	study					787:791	the encapsulation study	769:791	the encapsulation study	769:791	Rabbit articular chondrocytes were harvested and the cells from Passage 2 to 4 were used for the encapsulation study.
28551436	8	43	theme	spherical	1252:1260	arg1	phenotype					1262:1270	the spherical phenotype	1248:1270	the spherical phenotype in the less stiff gels	1248:1293	Although there was an increase in the percentage of viable encapsulated cells which also maintained the spherical phenotype in the less stiff gels, decreased expression of ECM markers- Collagen type II and Glycosaminoglycans was observed compared to the stiffer gels.
28551436	7	44	dep	181.47±19.77kPa	1072:1086	arg1	130.78±19.83kPa					1053:1067	130.78±19.83kPa	1053:1067	130.78±19.83kPa	1053:1067	The results of the study show that as the ratio of hyaluronic acid dialdehyde component was increased, the stiffness of the gels increased from 130.78±19.83kPa to 181.47±19.77kPa which was also evidenced from the decrease in gelling time.
28551436	5	45	theme	Rabbit	676:681	arg1	chondrocytes					693:704	Rabbit articular chondrocytes	676:704	Rabbit articular chondrocytes	676:704	Rabbit articular chondrocytes were harvested and the cells from Passage 2 to 4 were used for the encapsulation study.
28551436	5	46	from	Passage	740:746	arg1	cells					729:733	the cells	725:733	the cells from Passage 2 to 4	725:753	Rabbit articular chondrocytes were harvested and the cells from Passage 2 to 4 were used for the encapsulation study.
28551436	3	47	theme	gel	497:499	arg1	ratios					483:488	Three different ratios	467:488	Three different ratios of the gel	467:499	Three different ratios of the gel were prepared (10:1,10:3 and 10:5 CH-HDA) and characterized.
28551436	0	48	theme	dialdehyde	48:57	arg1	stiffness					10:18	stiffness	10:18	stiffness of chitosan-hyaluronic acid dialdehyde	10:57	Effect of stiffness of chitosan-hyaluronic acid dialdehyde hydrogels on the viability and growth of encapsulated chondrocytes.
28551436	5	49	theme	articular	683:691	arg1	chondrocytes					693:704	Rabbit articular chondrocytes	676:704	Rabbit articular chondrocytes	676:704	Rabbit articular chondrocytes were harvested and the cells from Passage 2 to 4 were used for the encapsulation study.
28551436	6	50	theme	chondrocytes	843:854	arg1	viability					798:806	viability	798:806	viability	798:806	The viability and ECM production of encapsulated chondrocytes were assessed at 7day, 14day and 28day post culture.
28551436	6	50	theme	chondrocytes	843:854	arg1	production					816:825	ECM production	812:825	ECM production	812:825	The viability and ECM production of encapsulated chondrocytes were assessed at 7day, 14day and 28day post culture.
28551436	7	51	theme	dialdehyde	976:985	arg1	component					987:995	hyaluronic acid dialdehyde component	960:995	hyaluronic acid dialdehyde component	960:995	The results of the study show that as the ratio of hyaluronic acid dialdehyde component was increased, the stiffness of the gels increased from 130.78±19.83kPa to 181.47±19.77kPa which was also evidenced from the decrease in gelling time.
28551436	1	52	theme	Substrate	127:135	arg1	elasticity					137:146	elasticity	137:146	elasticity	137:146	Substrate elasticity or stiffness can influence the phenotypic and functional characteristics of chondrocytes.
28551436	6	53	theme	encapsulated	830:841	arg1	chondrocytes					843:854	encapsulated chondrocytes	830:854	encapsulated chondrocytes	830:854	The viability and ECM production of encapsulated chondrocytes were assessed at 7day, 14day and 28day post culture.
28551436	2	54	theme	varying	277:283	arg1	compositions					295:306	varying stiffness compositions	277:306	varying stiffness compositions	277:306	This work aimed to study the effect of varying stiffness compositions of a two-component injectable hydrogel based on chitosan (CH) and oxidized hyaluronic acid (HDA) on the growth and functionality of encapsulated chondrocytes.
28551436	7	55	theme	component	987:995	arg1	ratio					951:955	the ratio	947:955	the ratio of hyaluronic acid dialdehyde component	947:995	The results of the study show that as the ratio of hyaluronic acid dialdehyde component was increased, the stiffness of the gels increased from 130.78±19.83kPa to 181.47±19.77kPa which was also evidenced from the decrease in gelling time.
28551436	8	56	theme	stiff	1284:1288	arg1	gels					1290:1293	the less stiff gels	1275:1293	the less stiff gels	1275:1293	Although there was an increase in the percentage of viable encapsulated cells which also maintained the spherical phenotype in the less stiff gels, decreased expression of ECM markers- Collagen type II and Glycosaminoglycans was observed compared to the stiffer gels.
28551436	8	57	theme	Collagen	1333:1340	arg1	expression					1306:1315	decreased expression	1296:1315	decreased expression of ECM markers- Collagen type II and Glycosaminoglycans	1296:1371	Although there was an increase in the percentage of viable encapsulated cells which also maintained the spherical phenotype in the less stiff gels, decreased expression of ECM markers- Collagen type II and Glycosaminoglycans was observed compared to the stiffer gels.
28551436	5	58	dep	Passage	740:746	arg1	to					750:751	to	750:751	to	750:751	Rabbit articular chondrocytes were harvested and the cells from Passage 2 to 4 were used for the encapsulation study.
28551436	9	59	theme	integrity	1543:1551	arg1	maintenance					1517:1527	maintenance	1517:1527	maintenance of phenotypic integrity and ECM production	1517:1570	These findings indicate that gel stiffness strongly impacts the chondrocyte microenvironment both in maintenance of phenotypic integrity and ECM production.
28551436	7	60	theme	hyaluronic	960:969	arg1	acid					971:974	hyaluronic acid	960:974	hyaluronic acid dialdehyde component	960:995	The results of the study show that as the ratio of hyaluronic acid dialdehyde component was increased, the stiffness of the gels increased from 130.78±19.83kPa to 181.47±19.77kPa which was also evidenced from the decrease in gelling time.
28551436	7	61	theme	acid	971:974	arg1	component					987:995	hyaluronic acid dialdehyde component	960:995	hyaluronic acid dialdehyde component	960:995	The results of the study show that as the ratio of hyaluronic acid dialdehyde component was increased, the stiffness of the gels increased from 130.78±19.83kPa to 181.47±19.77kPa which was also evidenced from the decrease in gelling time.
28551436	2	62	theme	hyaluronic	383:392	arg1	HDA					400:402	HDA	400:402	HDA	400:402	This work aimed to study the effect of varying stiffness compositions of a two-component injectable hydrogel based on chitosan (CH) and oxidized hyaluronic acid (HDA) on the growth and functionality of encapsulated chondrocytes.
28551436	2	62	theme	hyaluronic	383:392	arg1	acid					394:397	oxidized hyaluronic acid	374:397	oxidized hyaluronic acid (HDA)	374:403	This work aimed to study the effect of varying stiffness compositions of a two-component injectable hydrogel based on chitosan (CH) and oxidized hyaluronic acid (HDA) on the growth and functionality of encapsulated chondrocytes.
28551436	8	63	theme	encapsulated	1207:1218	arg1	cells					1220:1224	viable encapsulated cells	1200:1224	viable encapsulated cells	1200:1224	Although there was an increase in the percentage of viable encapsulated cells which also maintained the spherical phenotype in the less stiff gels, decreased expression of ECM markers- Collagen type II and Glycosaminoglycans was observed compared to the stiffer gels.
28551436	4	64	theme	force	611:615	arg1	curves					630:635	the force displacement curves	607:635	the force displacement curves using force spectroscopy AFM analysis	607:673	The stiffness of the gels was evaluated from the force displacement curves using force spectroscopy AFM analysis.
28551436	8	65	theme	viable	1200:1205	arg1	cells					1220:1224	viable encapsulated cells	1200:1224	viable encapsulated cells	1200:1224	Although there was an increase in the percentage of viable encapsulated cells which also maintained the spherical phenotype in the less stiff gels, decreased expression of ECM markers- Collagen type II and Glycosaminoglycans was observed compared to the stiffer gels.
28551436	0	66	theme	stiffness	10:18	arg1	Effect					0:5	Effect	0:5	Effect of stiffness of chitosan-hyaluronic acid dialdehyde	0:57	Effect of stiffness of chitosan-hyaluronic acid dialdehyde hydrogels on the viability and growth of encapsulated chondrocytes.
28551436	9	67	theme	ECM	1557:1559	arg1	production					1561:1570	ECM production	1557:1570	ECM production	1557:1570	These findings indicate that gel stiffness strongly impacts the chondrocyte microenvironment both in maintenance of phenotypic integrity and ECM production.
28551436	2	68	theme	oxidized	374:381	arg1	HDA					400:402	HDA	400:402	HDA	400:402	This work aimed to study the effect of varying stiffness compositions of a two-component injectable hydrogel based on chitosan (CH) and oxidized hyaluronic acid (HDA) on the growth and functionality of encapsulated chondrocytes.
28551436	2	68	theme	oxidized	374:381	arg1	acid					394:397	oxidized hyaluronic acid	374:397	oxidized hyaluronic acid (HDA)	374:403	This work aimed to study the effect of varying stiffness compositions of a two-component injectable hydrogel based on chitosan (CH) and oxidized hyaluronic acid (HDA) on the growth and functionality of encapsulated chondrocytes.
28551436	9	69	dep	maintenance	1517:1527	arg1	both					1509:1512	both	1509:1512	both	1509:1512	These findings indicate that gel stiffness strongly impacts the chondrocyte microenvironment both in maintenance of phenotypic integrity and ECM production.
25349979	0	0	theme	biomolecules	94:105	arg1	determination					71:83	the determination	67:83	the determination of small biomolecules	67:105	A lithium-rich composite metal oxide used as a SALDI-MS matrix for the determination of small biomolecules.
25349979	2	1	theme	background	278:287	arg1	interference					289:300	The low background interference	270:300	The low background interference	270:300	The low background interference and lithium-rich feature made it a highly efficient matrix for the analysis of small molecules with high reproducibility, sensitivity and strong salt tolerance.
25349979	1	2	theme	novel	164:168	arg1	oxide					139:143	A lithium-rich composite metal oxide	108:143	A lithium-rich composite metal oxide	108:143	A lithium-rich composite metal oxide was evaluated as a novel matrix for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-MS).
25349979	1	2	theme	novel	164:168	arg1	matrix					170:175	a novel matrix	162:175	a novel matrix for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-MS)	162:267	A lithium-rich composite metal oxide was evaluated as a novel matrix for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-MS).
25349979	2	3	theme	high	402:405	arg1	reproducibility					407:421	high reproducibility	402:421	high reproducibility	402:421	The low background interference and lithium-rich feature made it a highly efficient matrix for the analysis of small molecules with high reproducibility, sensitivity and strong salt tolerance.
25349979	0	4	theme	small	88:92	arg1	biomolecules					94:105	small biomolecules	88:105	small biomolecules	88:105	A lithium-rich composite metal oxide used as a SALDI-MS matrix for the determination of small biomolecules.
25349979	2	5	theme	low	274:276	arg1	interference					289:300	The low background interference	270:300	The low background interference	270:300	The low background interference and lithium-rich feature made it a highly efficient matrix for the analysis of small molecules with high reproducibility, sensitivity and strong salt tolerance.
25349979	0	6	theme	lithium-rich	2:13	arg1	oxide					31:35	A lithium-rich composite metal oxide	0:35	A lithium-rich composite metal oxide	0:35	A lithium-rich composite metal oxide used as a SALDI-MS matrix for the determination of small biomolecules.
25349979	0	7	used	used	37:40	arg2	oxide					31:35	A lithium-rich composite metal oxide	0:35	A lithium-rich composite metal oxide	0:35	A lithium-rich composite metal oxide used as a SALDI-MS matrix for the determination of small biomolecules.
25349979	2	8	theme	molecules	387:395	arg1	analysis					369:376	the analysis	365:376	the analysis of small molecules with high reproducibility, sensitivity and strong salt tolerance	365:460	The low background interference and lithium-rich feature made it a highly efficient matrix for the analysis of small molecules with high reproducibility, sensitivity and strong salt tolerance.
25349979	2	9	theme	small	381:385	arg1	molecules					387:395	small molecules	381:395	small molecules with high reproducibility, sensitivity and strong salt tolerance	381:460	The low background interference and lithium-rich feature made it a highly efficient matrix for the analysis of small molecules with high reproducibility, sensitivity and strong salt tolerance.
25349979	0	10	theme	metal	25:29	arg1	oxide					31:35	A lithium-rich composite metal oxide	0:35	A lithium-rich composite metal oxide	0:35	A lithium-rich composite metal oxide used as a SALDI-MS matrix for the determination of small biomolecules.
25349979	0	11	theme	composite	15:23	arg1	oxide					31:35	A lithium-rich composite metal oxide	0:35	A lithium-rich composite metal oxide	0:35	A lithium-rich composite metal oxide used as a SALDI-MS matrix for the determination of small biomolecules.
25349979	2	12	theme	lithium-rich	306:317	arg1	feature					319:325	lithium-rich feature	306:325	lithium-rich feature	306:325	The low background interference and lithium-rich feature made it a highly efficient matrix for the analysis of small molecules with high reproducibility, sensitivity and strong salt tolerance.
25349979	1	13	theme	matrix-assisted	181:195	arg1	spectrometry					245:256	matrix-assisted laser desorption/ionization time-of-flight mass spectrometry	181:256	matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-MS)	181:267	A lithium-rich composite metal oxide was evaluated as a novel matrix for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-MS).
25349979	1	13	theme	matrix-assisted	181:195	arg1	MALDI-MS					259:266	MALDI-MS	259:266	MALDI-MS	259:266	A lithium-rich composite metal oxide was evaluated as a novel matrix for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-MS).
25349979	1	14	theme	laser	197:201	arg1	spectrometry					245:256	matrix-assisted laser desorption/ionization time-of-flight mass spectrometry	181:256	matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-MS)	181:267	A lithium-rich composite metal oxide was evaluated as a novel matrix for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-MS).
25349979	1	14	theme	laser	197:201	arg1	MALDI-MS					259:266	MALDI-MS	259:266	MALDI-MS	259:266	A lithium-rich composite metal oxide was evaluated as a novel matrix for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-MS).
25349979	2	15	theme	salt	447:450	arg1	tolerance					452:460	strong salt tolerance	440:460	strong salt tolerance	440:460	The low background interference and lithium-rich feature made it a highly efficient matrix for the analysis of small molecules with high reproducibility, sensitivity and strong salt tolerance.
25349979	1	16	theme	desorption/ionization	203:223	arg1	spectrometry					245:256	matrix-assisted laser desorption/ionization time-of-flight mass spectrometry	181:256	matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-MS)	181:267	A lithium-rich composite metal oxide was evaluated as a novel matrix for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-MS).
25349979	1	16	theme	desorption/ionization	203:223	arg1	MALDI-MS					259:266	MALDI-MS	259:266	MALDI-MS	259:266	A lithium-rich composite metal oxide was evaluated as a novel matrix for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-MS).
25349979	2	17	theme	efficient	344:352	arg1	matrix					354:359	a highly efficient matrix	335:359	a highly efficient matrix	335:359	The low background interference and lithium-rich feature made it a highly efficient matrix for the analysis of small molecules with high reproducibility, sensitivity and strong salt tolerance.
25349979	1	18	theme	lithium-rich	110:121	arg1	oxide					139:143	A lithium-rich composite metal oxide	108:143	A lithium-rich composite metal oxide	108:143	A lithium-rich composite metal oxide was evaluated as a novel matrix for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-MS).
25349979	1	18	theme	lithium-rich	110:121	arg1	matrix					170:175	a novel matrix	162:175	a novel matrix for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-MS)	162:267	A lithium-rich composite metal oxide was evaluated as a novel matrix for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-MS).
25349979	1	19	theme	time-of-flight	225:238	arg1	spectrometry					245:256	matrix-assisted laser desorption/ionization time-of-flight mass spectrometry	181:256	matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-MS)	181:267	A lithium-rich composite metal oxide was evaluated as a novel matrix for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-MS).
25349979	1	19	theme	time-of-flight	225:238	arg1	MALDI-MS					259:266	MALDI-MS	259:266	MALDI-MS	259:266	A lithium-rich composite metal oxide was evaluated as a novel matrix for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-MS).
25349979	1	20	theme	composite	123:131	arg1	oxide					139:143	A lithium-rich composite metal oxide	108:143	A lithium-rich composite metal oxide	108:143	A lithium-rich composite metal oxide was evaluated as a novel matrix for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-MS).
25349979	1	20	theme	composite	123:131	arg1	matrix					170:175	a novel matrix	162:175	a novel matrix for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-MS)	162:267	A lithium-rich composite metal oxide was evaluated as a novel matrix for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-MS).
25349979	1	21	theme	mass	240:243	arg1	spectrometry					245:256	matrix-assisted laser desorption/ionization time-of-flight mass spectrometry	181:256	matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-MS)	181:267	A lithium-rich composite metal oxide was evaluated as a novel matrix for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-MS).
25349979	1	21	theme	mass	240:243	arg1	MALDI-MS					259:266	MALDI-MS	259:266	MALDI-MS	259:266	A lithium-rich composite metal oxide was evaluated as a novel matrix for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-MS).
25349979	1	22	theme	metal	133:137	arg1	oxide					139:143	A lithium-rich composite metal oxide	108:143	A lithium-rich composite metal oxide	108:143	A lithium-rich composite metal oxide was evaluated as a novel matrix for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-MS).
25349979	1	22	theme	metal	133:137	arg1	matrix					170:175	a novel matrix	162:175	a novel matrix for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-MS)	162:267	A lithium-rich composite metal oxide was evaluated as a novel matrix for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-MS).
25349979	0	23	theme	SALDI-MS	47:54	arg1	matrix					56:61	a SALDI-MS matrix	45:61	a SALDI-MS matrix for the determination of small biomolecules	45:105	A lithium-rich composite metal oxide used as a SALDI-MS matrix for the determination of small biomolecules.
25349979	2	24	with	molecules	387:395	arg1	tolerance					452:460	strong salt tolerance	440:460	strong salt tolerance	440:460	The low background interference and lithium-rich feature made it a highly efficient matrix for the analysis of small molecules with high reproducibility, sensitivity and strong salt tolerance.
25349979	2	24	with	molecules	387:395	arg1	sensitivity					424:434	sensitivity	424:434	sensitivity	424:434	The low background interference and lithium-rich feature made it a highly efficient matrix for the analysis of small molecules with high reproducibility, sensitivity and strong salt tolerance.
25349979	2	24	with	molecules	387:395	arg1	reproducibility					407:421	high reproducibility	402:421	high reproducibility	402:421	The low background interference and lithium-rich feature made it a highly efficient matrix for the analysis of small molecules with high reproducibility, sensitivity and strong salt tolerance.
25349979	2	25	theme	strong	440:445	arg1	tolerance					452:460	strong salt tolerance	440:460	strong salt tolerance	440:460	The low background interference and lithium-rich feature made it a highly efficient matrix for the analysis of small molecules with high reproducibility, sensitivity and strong salt tolerance.
24893649	5	0	theme	hydrophilic	709:719	arg1	fibers					721:726	hydrophilic fibers	709:726	hydrophilic fibers	709:726	Our method is specially suited for the manufacturing of rigid and robust preforms of hydrophilic fibers.
24893649	8	1	theme	adjacent	1080:1087	arg1	cellulose					1099:1107	the adjacent bacterial cellulose	1076:1107	the adjacent bacterial cellulose	1076:1107	When the loose fiber-bacterial cellulose suspension is filtered and dried, the adjacent bacterial cellulose forms a network and hornified to hold the otherwise loose fibers together.
24893649	10	2	theme	bacterial	1393:1401	arg1	network					1413:1419	the bacterial cellulose network	1389:1419	the bacterial cellulose network	1389:1419	This can be attributed to the high stiffness and strength of the bacterial cellulose network.
24893649	11	3	theme	transfer	1612:1619	arg1	RTM					1630:1632	RTM	1630:1632	RTM	1630:1632	With this preform, renewable high performance hierarchical composites can also be manufactured by using conventional composite production methods, such as resin film infusion (RFI) or resin transfer molding (RTM).
24893649	11	3	theme	transfer	1612:1619	arg1	molding					1621:1627	resin transfer molding	1606:1627	resin transfer molding (RTM)	1606:1633	With this preform, renewable high performance hierarchical composites can also be manufactured by using conventional composite production methods, such as resin film infusion (RFI) or resin transfer molding (RTM).
24893649	1	4	theme	preforms	151:158	arg1	method					96:101	A novel method	88:101	A novel method of manufacturing rigid and robust natural fiber preforms	88:158	A novel method of manufacturing rigid and robust natural fiber preforms is presented here.
24893649	5	5	theme	robust	690:695	arg1	preforms					697:704	rigid and robust preforms	680:704	rigid and robust preforms of hydrophilic fibers	680:726	Our method is specially suited for the manufacturing of rigid and robust preforms of hydrophilic fibers.
24893649	5	6	theme	fibers	721:726	arg1	preforms					697:704	rigid and robust preforms	680:704	rigid and robust preforms of hydrophilic fibers	680:726	Our method is specially suited for the manufacturing of rigid and robust preforms of hydrophilic fibers.
24893649	8	7	dep	network	1117:1123	arg1	a					1115:1115	a	1115:1115	a	1115:1115	When the loose fiber-bacterial cellulose suspension is filtered and dried, the adjacent bacterial cellulose forms a network and hornified to hold the otherwise loose fibers together.
24893649	6	8	theme	such	766:769	arg1	fibers					771:776	such fibers	766:776	such fibers	766:776	The porous and hydrophilic nature of such fibers results in significant water uptake, drawing in the bacterial cellulose dispersed in the suspension.
24893649	11	9	theme	high	1451:1454	arg1	composites					1481:1490	renewable high performance hierarchical composites	1441:1490	renewable high performance hierarchical composites	1441:1490	With this preform, renewable high performance hierarchical composites can also be manufactured by using conventional composite production methods, such as resin film infusion (RFI) or resin transfer molding (RTM).
24893649	11	10	theme	composite	1539:1547	arg1	molding					1621:1627	resin transfer molding	1606:1627	resin transfer molding (RTM)	1606:1633	With this preform, renewable high performance hierarchical composites can also be manufactured by using conventional composite production methods, such as resin film infusion (RFI) or resin transfer molding (RTM).
24893649	11	10	theme	composite	1539:1547	arg1	methods					1560:1566	conventional composite production methods	1526:1566	conventional composite production methods	1526:1566	With this preform, renewable high performance hierarchical composites can also be manufactured by using conventional composite production methods, such as resin film infusion (RFI) or resin transfer molding (RTM).
24893649	11	10	theme	composite	1539:1547	arg1	infusion					1588:1595	resin film infusion	1577:1595	resin film infusion (RFI)	1577:1601	With this preform, renewable high performance hierarchical composites can also be manufactured by using conventional composite production methods, such as resin film infusion (RFI) or resin transfer molding (RTM).
24893649	12	11	theme	double	1720:1725	arg1	infusion					1753:1760	double bag vacuum assisted resin infusion	1720:1760	double bag vacuum assisted resin infusion	1720:1760	Here, we also describe the manufacturing of renewable hierarchical composites using double bag vacuum assisted resin infusion.
24893649	3	12	theme	drying	494:499	arg1	step					501:504	a drying step	492:504	a drying step	492:504	The fiber and nanocellulose suspension is then filtered (using vacuum or gravity) and the wet filter cake pressed to squeeze out any excess water, followed by a drying step.
24893649	11	13	theme	performance	1456:1466	arg1	composites					1481:1490	renewable high performance hierarchical composites	1441:1490	renewable high performance hierarchical composites	1441:1490	With this preform, renewable high performance hierarchical composites can also be manufactured by using conventional composite production methods, such as resin film infusion (RFI) or resin transfer molding (RTM).
24893649	3	14	theme	nanocellulose	347:359	arg1	suspension					361:370	The fiber and nanocellulose suspension	333:370	suspension	361:370	The fiber and nanocellulose suspension is then filtered (using vacuum or gravity) and the wet filter cake pressed to squeeze out any excess water, followed by a drying step.
24893649	9	15	theme	significant	1255:1265	arg1	increase					1267:1274	a significant increase	1253:1274	a significant increase of the mechanical properties of the fiber preforms	1253:1325	The introduction of bacterial cellulose into the preform resulted in a significant increase of the mechanical properties of the fiber preforms.
24893649	11	16	theme	resin	1577:1581	arg1	RFI					1598:1600	RFI	1598:1600	RFI	1598:1600	With this preform, renewable high performance hierarchical composites can also be manufactured by using conventional composite production methods, such as resin film infusion (RFI) or resin transfer molding (RTM).
24893649	11	16	theme	resin	1577:1581	arg1	infusion					1588:1595	resin film infusion	1577:1595	resin film infusion (RFI)	1577:1601	With this preform, renewable high performance hierarchical composites can also be manufactured by using conventional composite production methods, such as resin film infusion (RFI) or resin transfer molding (RTM).
24893649	3	17	theme	excess	466:471	arg1	water					473:477	any excess water	462:477	any excess water	462:477	The fiber and nanocellulose suspension is then filtered (using vacuum or gravity) and the wet filter cake pressed to squeeze out any excess water, followed by a drying step.
24893649	12	18	theme	hierarchical	1690:1701	arg1	composites					1703:1712	renewable hierarchical composites	1680:1712	renewable hierarchical composites	1680:1712	Here, we also describe the manufacturing of renewable hierarchical composites using double bag vacuum assisted resin infusion.
24893649	11	19	theme	conventional	1526:1537	arg1	molding					1621:1627	resin transfer molding	1606:1627	resin transfer molding (RTM)	1606:1633	With this preform, renewable high performance hierarchical composites can also be manufactured by using conventional composite production methods, such as resin film infusion (RFI) or resin transfer molding (RTM).
24893649	11	19	theme	conventional	1526:1537	arg1	methods					1560:1566	conventional composite production methods	1526:1566	conventional composite production methods	1526:1566	With this preform, renewable high performance hierarchical composites can also be manufactured by using conventional composite production methods, such as resin film infusion (RFI) or resin transfer molding (RTM).
24893649	11	19	theme	conventional	1526:1537	arg1	infusion					1588:1595	resin film infusion	1577:1595	resin film infusion (RFI)	1577:1601	With this preform, renewable high performance hierarchical composites can also be manufactured by using conventional composite production methods, such as resin film infusion (RFI) or resin transfer molding (RTM).
24893649	6	20	theme	bacterial	830:838	arg1	cellulose					840:848	the bacterial cellulose	826:848	the bacterial cellulose dispersed in the suspension	826:876	The porous and hydrophilic nature of such fibers results in significant water uptake, drawing in the bacterial cellulose dispersed in the suspension.
24893649	6	21	theme	hydrophilic	744:754	arg1	nature					756:761	The porous and hydrophilic nature	729:761	The porous and hydrophilic nature of such fibers	729:776	The porous and hydrophilic nature of such fibers results in significant water uptake, drawing in the bacterial cellulose dispersed in the suspension.
24893649	3	22	theme	fiber	337:341	arg1	suspension					361:370	The fiber and nanocellulose suspension	333:370	suspension	361:370	The fiber and nanocellulose suspension is then filtered (using vacuum or gravity) and the wet filter cake pressed to squeeze out any excess water, followed by a drying step.
24893649	11	23	theme	resin	1606:1610	arg1	RTM					1630:1632	RTM	1630:1632	RTM	1630:1632	With this preform, renewable high performance hierarchical composites can also be manufactured by using conventional composite production methods, such as resin film infusion (RFI) or resin transfer molding (RTM).
24893649	11	23	theme	resin	1606:1610	arg1	molding					1621:1627	resin transfer molding	1606:1627	resin transfer molding (RTM)	1606:1633	With this preform, renewable high performance hierarchical composites can also be manufactured by using conventional composite production methods, such as resin film infusion (RFI) or resin transfer molding (RTM).
24893649	8	24	theme	loose	1161:1165	arg1	fibers					1167:1172	the otherwise loose fibers	1147:1172	the otherwise loose fibers	1147:1172	When the loose fiber-bacterial cellulose suspension is filtered and dried, the adjacent bacterial cellulose forms a network and hornified to hold the otherwise loose fibers together.
24893649	7	25	theme	bacterial	883:891	arg1	cellulose					893:901	The bacterial cellulose	879:901	The bacterial cellulose	879:901	The bacterial cellulose will then be filtered against the surface of these fibers, forming a bacterial cellulose coating.
24893649	2	26	theme	sisal	250:254	arg1	fibers					256:261	loose and short sisal fibers	234:261	loose and short sisal fibers	234:261	This method is based on a papermaking process, whereby loose and short sisal fibers are dispersed into a water suspension containing bacterial cellulose.
24893649	12	27	theme	composites	1703:1712	arg1	manufacturing					1663:1675	the manufacturing	1659:1675	the manufacturing of renewable hierarchical composites	1659:1712	Here, we also describe the manufacturing of renewable hierarchical composites using double bag vacuum assisted resin infusion.
24893649	4	28	theme	natural	599:605	arg1	fibers					607:612	the loose natural fibers	589:612	the loose natural fibers	589:612	This will result in the hornification of the bacterial cellulose network, holding the loose natural fibers together.
24893649	8	29	theme	bacterial	1089:1097	arg1	cellulose					1099:1107	the adjacent bacterial cellulose	1076:1107	the adjacent bacterial cellulose	1076:1107	When the loose fiber-bacterial cellulose suspension is filtered and dried, the adjacent bacterial cellulose forms a network and hornified to hold the otherwise loose fibers together.
24893649	8	30	theme	loose	1010:1014	arg1	suspension					1042:1051	the loose fiber-bacterial cellulose suspension	1006:1051	the loose fiber-bacterial cellulose suspension	1006:1051	When the loose fiber-bacterial cellulose suspension is filtered and dried, the adjacent bacterial cellulose forms a network and hornified to hold the otherwise loose fibers together.
24893649	6	31	theme	porous	733:738	arg1	nature					756:761	The porous and hydrophilic nature	729:761	The porous and hydrophilic nature of such fibers	729:776	The porous and hydrophilic nature of such fibers results in significant water uptake, drawing in the bacterial cellulose dispersed in the suspension.
24893649	9	32	theme	mechanical	1283:1292	arg1	properties					1294:1303	the mechanical properties	1279:1303	the mechanical properties of the fiber preforms	1279:1325	The introduction of bacterial cellulose into the preform resulted in a significant increase of the mechanical properties of the fiber preforms.
24893649	10	33	theme	cellulose	1403:1411	arg1	network					1413:1419	the bacterial cellulose network	1389:1419	the bacterial cellulose network	1389:1419	This can be attributed to the high stiffness and strength of the bacterial cellulose network.
24893649	10	34	theme	high	1358:1361	arg1	stiffness					1363:1371	high stiffness	1358:1371	high stiffness	1358:1371	This can be attributed to the high stiffness and strength of the bacterial cellulose network.
24893649	1	35	theme	novel	90:94	arg1	method					96:101	A novel method	88:101	A novel method of manufacturing rigid and robust natural fiber preforms	88:158	A novel method of manufacturing rigid and robust natural fiber preforms is presented here.
24893649	0	36	theme	natural	24:30	arg1	preforms					38:45	robust natural fiber preforms	17:45	robust natural fiber preforms	17:45	Manufacturing of robust natural fiber preforms utilizing bacterial cellulose as binder.
24893649	9	37	theme	properties	1294:1303	arg1	increase					1267:1274	a significant increase	1253:1274	a significant increase of the mechanical properties of the fiber preforms	1253:1325	The introduction of bacterial cellulose into the preform resulted in a significant increase of the mechanical properties of the fiber preforms.
24893649	8	38	theme	cellulose	1032:1040	arg1	suspension					1042:1051	the loose fiber-bacterial cellulose suspension	1006:1051	the loose fiber-bacterial cellulose suspension	1006:1051	When the loose fiber-bacterial cellulose suspension is filtered and dried, the adjacent bacterial cellulose forms a network and hornified to hold the otherwise loose fibers together.
24893649	10	39	theme	network	1413:1419	arg1	strength					1377:1384	strength	1377:1384	strength	1377:1384	This can be attributed to the high stiffness and strength of the bacterial cellulose network.
24893649	10	39	theme	network	1413:1419	arg1	stiffness					1363:1371	high stiffness	1358:1371	high stiffness	1358:1371	This can be attributed to the high stiffness and strength of the bacterial cellulose network.
24893649	2	40	theme	water	284:288	arg1	suspension					290:299	a water suspension	282:299	a water suspension containing bacterial cellulose	282:330	This method is based on a papermaking process, whereby loose and short sisal fibers are dispersed into a water suspension containing bacterial cellulose.
24893649	12	41	theme	assisted	1738:1745	arg1	infusion					1753:1760	double bag vacuum assisted resin infusion	1720:1760	double bag vacuum assisted resin infusion	1720:1760	Here, we also describe the manufacturing of renewable hierarchical composites using double bag vacuum assisted resin infusion.
24893649	8	42	theme	fiber-bacterial	1016:1030	arg1	suspension					1042:1051	the loose fiber-bacterial cellulose suspension	1006:1051	the loose fiber-bacterial cellulose suspension	1006:1051	When the loose fiber-bacterial cellulose suspension is filtered and dried, the adjacent bacterial cellulose forms a network and hornified to hold the otherwise loose fibers together.
24893649	0	43	theme	preforms	38:45	arg1	Manufacturing					0:12	Manufacturing	0:12	Manufacturing of robust natural fiber preforms	0:45	Manufacturing of robust natural fiber preforms utilizing bacterial cellulose as binder.
24893649	4	44	theme	loose	593:597	arg1	fibers					607:612	the loose natural fibers	589:612	the loose natural fibers	589:612	This will result in the hornification of the bacterial cellulose network, holding the loose natural fibers together.
24893649	3	45	theme	wet	423:425	arg1	cake					434:437	the wet filter cake	419:437	the wet filter cake	419:437	The fiber and nanocellulose suspension is then filtered (using vacuum or gravity) and the wet filter cake pressed to squeeze out any excess water, followed by a drying step.
24893649	0	46	theme	fiber	32:36	arg1	preforms					38:45	robust natural fiber preforms	17:45	robust natural fiber preforms	17:45	Manufacturing of robust natural fiber preforms utilizing bacterial cellulose as binder.
24893649	9	47	theme	fiber	1312:1316	arg1	preforms					1318:1325	the fiber preforms	1308:1325	the fiber preforms	1308:1325	The introduction of bacterial cellulose into the preform resulted in a significant increase of the mechanical properties of the fiber preforms.
24893649	5	48	theme	rigid	680:684	arg1	preforms					697:704	rigid and robust preforms	680:704	rigid and robust preforms of hydrophilic fibers	680:726	Our method is specially suited for the manufacturing of rigid and robust preforms of hydrophilic fibers.
24893649	3	49	theme	filter	427:432	arg1	cake					434:437	the wet filter cake	419:437	the wet filter cake	419:437	The fiber and nanocellulose suspension is then filtered (using vacuum or gravity) and the wet filter cake pressed to squeeze out any excess water, followed by a drying step.
24893649	0	50	theme	bacterial	57:65	arg1	cellulose					67:75	bacterial cellulose	57:75	bacterial cellulose	57:75	Manufacturing of robust natural fiber preforms utilizing bacterial cellulose as binder.
24893649	9	51	theme	preforms	1318:1325	arg1	properties					1294:1303	the mechanical properties	1279:1303	the mechanical properties of the fiber preforms	1279:1325	The introduction of bacterial cellulose into the preform resulted in a significant increase of the mechanical properties of the fiber preforms.
24893649	7	52	theme	cellulose	982:990	arg1	coating					992:998	a bacterial cellulose coating	970:998	a bacterial cellulose coating	970:998	The bacterial cellulose will then be filtered against the surface of these fibers, forming a bacterial cellulose coating.
24893649	6	53	theme	water	801:805	arg1	uptake					807:812	significant water uptake	789:812	significant water uptake	789:812	The porous and hydrophilic nature of such fibers results in significant water uptake, drawing in the bacterial cellulose dispersed in the suspension.
24893649	2	54	theme	papermaking	205:215	arg1	process					217:223	a papermaking process	203:223	a papermaking process	203:223	This method is based on a papermaking process, whereby loose and short sisal fibers are dispersed into a water suspension containing bacterial cellulose.
24893649	1	55	theme	manufacturing	106:118	arg1	preforms					151:158	manufacturing rigid and robust natural fiber preforms	106:158	manufacturing rigid and robust natural fiber preforms	106:158	A novel method of manufacturing rigid and robust natural fiber preforms is presented here.
24893649	9	56	theme	bacterial	1204:1212	arg1	cellulose					1214:1222	bacterial cellulose	1204:1222	bacterial cellulose	1204:1222	The introduction of bacterial cellulose into the preform resulted in a significant increase of the mechanical properties of the fiber preforms.
24893649	6	57	theme	significant	789:799	arg1	uptake					807:812	significant water uptake	789:812	significant water uptake	789:812	The porous and hydrophilic nature of such fibers results in significant water uptake, drawing in the bacterial cellulose dispersed in the suspension.
24893649	11	58	theme	production	1549:1558	arg1	molding					1621:1627	resin transfer molding	1606:1627	resin transfer molding (RTM)	1606:1633	With this preform, renewable high performance hierarchical composites can also be manufactured by using conventional composite production methods, such as resin film infusion (RFI) or resin transfer molding (RTM).
24893649	11	58	theme	production	1549:1558	arg1	methods					1560:1566	conventional composite production methods	1526:1566	conventional composite production methods	1526:1566	With this preform, renewable high performance hierarchical composites can also be manufactured by using conventional composite production methods, such as resin film infusion (RFI) or resin transfer molding (RTM).
24893649	11	58	theme	production	1549:1558	arg1	infusion					1588:1595	resin film infusion	1577:1595	resin film infusion (RFI)	1577:1601	With this preform, renewable high performance hierarchical composites can also be manufactured by using conventional composite production methods, such as resin film infusion (RFI) or resin transfer molding (RTM).
24893649	1	59	theme	rigid	120:124	arg1	preforms					151:158	manufacturing rigid and robust natural fiber preforms	106:158	manufacturing rigid and robust natural fiber preforms	106:158	A novel method of manufacturing rigid and robust natural fiber preforms is presented here.
24893649	3	60	dep	pressed	439:445	arg1	followed					480:487	followed	480:487	followed by a drying step	480:504	The fiber and nanocellulose suspension is then filtered (using vacuum or gravity) and the wet filter cake pressed to squeeze out any excess water, followed by a drying step.
24893649	9	61	theme	cellulose	1214:1222	arg1	introduction					1188:1199	The introduction	1184:1199	The introduction of bacterial cellulose into the preform	1184:1239	The introduction of bacterial cellulose into the preform resulted in a significant increase of the mechanical properties of the fiber preforms.
24893649	2	62	theme	short	244:248	arg1	fibers					256:261	loose and short sisal fibers	234:261	loose and short sisal fibers	234:261	This method is based on a papermaking process, whereby loose and short sisal fibers are dispersed into a water suspension containing bacterial cellulose.
24893649	7	63	theme	fibers	954:959	arg1	surface					937:943	the surface	933:943	the surface of these fibers	933:959	The bacterial cellulose will then be filtered against the surface of these fibers, forming a bacterial cellulose coating.
24893649	2	64	theme	bacterial	312:320	arg1	cellulose					322:330	bacterial cellulose	312:330	bacterial cellulose	312:330	This method is based on a papermaking process, whereby loose and short sisal fibers are dispersed into a water suspension containing bacterial cellulose.
24893649	11	65	theme	renewable	1441:1449	arg1	composites					1481:1490	renewable high performance hierarchical composites	1441:1490	renewable high performance hierarchical composites	1441:1490	With this preform, renewable high performance hierarchical composites can also be manufactured by using conventional composite production methods, such as resin film infusion (RFI) or resin transfer molding (RTM).
24893649	4	66	theme	bacterial	552:560	arg1	network					572:578	the bacterial cellulose network	548:578	the bacterial cellulose network	548:578	This will result in the hornification of the bacterial cellulose network, holding the loose natural fibers together.
24893649	7	67	theme	bacterial	972:980	arg1	coating					992:998	a bacterial cellulose coating	970:998	a bacterial cellulose coating	970:998	The bacterial cellulose will then be filtered against the surface of these fibers, forming a bacterial cellulose coating.
24893649	11	68	theme	film	1583:1586	arg1	RFI					1598:1600	RFI	1598:1600	RFI	1598:1600	With this preform, renewable high performance hierarchical composites can also be manufactured by using conventional composite production methods, such as resin film infusion (RFI) or resin transfer molding (RTM).
24893649	11	68	theme	film	1583:1586	arg1	infusion					1588:1595	resin film infusion	1577:1595	resin film infusion (RFI)	1577:1601	With this preform, renewable high performance hierarchical composites can also be manufactured by using conventional composite production methods, such as resin film infusion (RFI) or resin transfer molding (RTM).
24893649	1	69	theme	robust	130:135	arg1	preforms					151:158	manufacturing rigid and robust natural fiber preforms	106:158	manufacturing rigid and robust natural fiber preforms	106:158	A novel method of manufacturing rigid and robust natural fiber preforms is presented here.
24893649	12	70	theme	vacuum	1731:1736	arg1	infusion					1753:1760	double bag vacuum assisted resin infusion	1720:1760	double bag vacuum assisted resin infusion	1720:1760	Here, we also describe the manufacturing of renewable hierarchical composites using double bag vacuum assisted resin infusion.
24893649	5	71	theme	preforms	697:704	arg1	manufacturing					663:675	the manufacturing	659:675	the manufacturing of rigid and robust preforms of hydrophilic fibers	659:726	Our method is specially suited for the manufacturing of rigid and robust preforms of hydrophilic fibers.
24893649	6	72	theme	fibers	771:776	arg1	nature					756:761	The porous and hydrophilic nature	729:761	The porous and hydrophilic nature of such fibers	729:776	The porous and hydrophilic nature of such fibers results in significant water uptake, drawing in the bacterial cellulose dispersed in the suspension.
24893649	2	73	theme	loose	234:238	arg1	fibers					256:261	loose and short sisal fibers	234:261	loose and short sisal fibers	234:261	This method is based on a papermaking process, whereby loose and short sisal fibers are dispersed into a water suspension containing bacterial cellulose.
24893649	12	74	theme	bag	1727:1729	arg1	infusion					1753:1760	double bag vacuum assisted resin infusion	1720:1760	double bag vacuum assisted resin infusion	1720:1760	Here, we also describe the manufacturing of renewable hierarchical composites using double bag vacuum assisted resin infusion.
24893649	1	75	theme	natural	137:143	arg1	preforms					151:158	manufacturing rigid and robust natural fiber preforms	106:158	manufacturing rigid and robust natural fiber preforms	106:158	A novel method of manufacturing rigid and robust natural fiber preforms is presented here.
24893649	12	76	theme	renewable	1680:1688	arg1	composites					1703:1712	renewable hierarchical composites	1680:1712	renewable hierarchical composites	1680:1712	Here, we also describe the manufacturing of renewable hierarchical composites using double bag vacuum assisted resin infusion.
24893649	4	77	theme	network	572:578	arg1	hornification					531:543	the hornification	527:543	the hornification of the bacterial cellulose network	527:578	This will result in the hornification of the bacterial cellulose network, holding the loose natural fibers together.
24893649	2	78	contain	containing	301:310	arg2	cellulose					322:330	bacterial cellulose	312:330	bacterial cellulose	312:330	This method is based on a papermaking process, whereby loose and short sisal fibers are dispersed into a water suspension containing bacterial cellulose.
24893649	2	78	contain	containing	301:310	arg1	suspension					290:299	a water suspension	282:299	a water suspension containing bacterial cellulose	282:330	This method is based on a papermaking process, whereby loose and short sisal fibers are dispersed into a water suspension containing bacterial cellulose.
24893649	0	79	theme	robust	17:22	arg1	preforms					38:45	robust natural fiber preforms	17:45	robust natural fiber preforms	17:45	Manufacturing of robust natural fiber preforms utilizing bacterial cellulose as binder.
24893649	12	80	theme	resin	1747:1751	arg1	infusion					1753:1760	double bag vacuum assisted resin infusion	1720:1760	double bag vacuum assisted resin infusion	1720:1760	Here, we also describe the manufacturing of renewable hierarchical composites using double bag vacuum assisted resin infusion.
24893649	1	81	theme	fiber	145:149	arg1	preforms					151:158	manufacturing rigid and robust natural fiber preforms	106:158	manufacturing rigid and robust natural fiber preforms	106:158	A novel method of manufacturing rigid and robust natural fiber preforms is presented here.
24893649	11	82	theme	hierarchical	1468:1479	arg1	composites					1481:1490	renewable high performance hierarchical composites	1441:1490	renewable high performance hierarchical composites	1441:1490	With this preform, renewable high performance hierarchical composites can also be manufactured by using conventional composite production methods, such as resin film infusion (RFI) or resin transfer molding (RTM).
24893649	4	83	theme	cellulose	562:570	arg1	network					572:578	the bacterial cellulose network	548:578	the bacterial cellulose network	548:578	This will result in the hornification of the bacterial cellulose network, holding the loose natural fibers together.
25498637	8	0	theme	different	1027:1035	arg1	films					1066:1070	different tunicate CN-GM nanocomposite films	1027:1070	different tunicate CN-GM nanocomposite films	1027:1070	This is the first systematic study of different tunicate CN-GM nanocomposite films and the first ever for spruce GM.
25498637	5	1	theme	ACN	565:567	arg1	fibrils					569:575	TCN and ACN fibrils	557:575	TCN and ACN fibrils	557:575	For CN-films, TCN and ACN fibrils were stretched and parallel to each other due to surface charges.
25498637	0	2	with	preparation	33:43	arg1	glucomannans					86:97	glucomannans	86:97	glucomannans	86:97	Tunicate cellulose nanocrystals: preparation, neat films and nanocomposite films with glucomannans.
25498637	1	3	theme	acid	223:226	arg1	hydrolysis					228:237	acid hydrolysis	223:237	acid hydrolysis (ACN)	223:243	Cellulose nanocrystals (CNs) were prepared from tunicate by enzymatic hydrolysis (ECN), TEMPO-mediated oxidation (TCN) and acid hydrolysis (ACN).
25498637	1	3	theme	acid	223:226	arg1	ACN					240:242	ACN	240:242	ACN	240:242	Cellulose nanocrystals (CNs) were prepared from tunicate by enzymatic hydrolysis (ECN), TEMPO-mediated oxidation (TCN) and acid hydrolysis (ACN).
25498637	7	4	theme	good	857:860	arg1	properties					894:903	good thermal, optical and mechanical properties	857:903	good thermal, optical and mechanical properties; the last ones are apparently better than similar films reported in the literature	857:986	The composite films had good thermal, optical and mechanical properties; the last ones are apparently better than similar films reported in the literature.
25498637	3	5	theme	increased	441:449	arg1	crystallinity					451:463	increased crystallinity	441:463	increased crystallinity	441:463	Different CNs were obtained with a yield of ECN>TCN>ACN with corresponding order of decreased Mw but increased crystallinity.
25498637	8	6	theme	CN-GM	1046:1050	arg1	films					1066:1070	different tunicate CN-GM nanocomposite films	1027:1070	different tunicate CN-GM nanocomposite films	1027:1070	This is the first systematic study of different tunicate CN-GM nanocomposite films and the first ever for spruce GM.
25498637	6	7	theme	angle	803:807	arg1	changes					726:732	changes	726:732	changes of crystallinity, specific surface area, fibril diameter and contact angle compared with CN films	726:830	For CN-GM films, both components interacted strongly with each other, resulting in changes of crystallinity, specific surface area, fibril diameter and contact angle compared with CN films.
25498637	0	8	with	films	75:79	arg1	glucomannans					86:97	glucomannans	86:97	glucomannans	86:97	Tunicate cellulose nanocrystals: preparation, neat films and nanocomposite films with glucomannans.
25498637	7	9	theme	optical	871:877	arg1	properties					894:903	good thermal, optical and mechanical properties	857:903	good thermal, optical and mechanical properties; the last ones are apparently better than similar films reported in the literature	857:986	The composite films had good thermal, optical and mechanical properties; the last ones are apparently better than similar films reported in the literature.
25498637	6	10	theme	CN-GM	647:651	arg1	films					653:657	CN-GM films	647:657	CN-GM films	647:657	For CN-GM films, both components interacted strongly with each other, resulting in changes of crystallinity, specific surface area, fibril diameter and contact angle compared with CN films.
25498637	6	11	theme	contact	795:801	arg1	angle					803:807	contact angle	795:807	contact angle	795:807	For CN-GM films, both components interacted strongly with each other, resulting in changes of crystallinity, specific surface area, fibril diameter and contact angle compared with CN films.
25498637	7	12	theme	last	910:913	arg1	ones					915:918	the last ones	906:918	the last ones	906:918	The composite films had good thermal, optical and mechanical properties; the last ones are apparently better than similar films reported in the literature.
25498637	7	12	theme	last	910:913	arg1	better					935:940	better	935:940	better	935:940	The composite films had good thermal, optical and mechanical properties; the last ones are apparently better than similar films reported in the literature.
25498637	7	13	dep	properties	894:903	arg1	ones					915:918	the last ones	906:918	the last ones	906:918	The composite films had good thermal, optical and mechanical properties; the last ones are apparently better than similar films reported in the literature.
25498637	7	13	dep	properties	894:903	arg1	better					935:940	better	935:940	better	935:940	The composite films had good thermal, optical and mechanical properties; the last ones are apparently better than similar films reported in the literature.
25498637	8	14	theme	films	1066:1070	arg1	study					1018:1022	the first systematic study	997:1022	the first systematic study of different tunicate CN-GM nanocomposite films	997:1070	This is the first systematic study of different tunicate CN-GM nanocomposite films and the first ever for spruce GM.
25498637	8	14	theme	films	1066:1070	arg1	This					989:992	This	989:992	This	989:992	This is the first systematic study of different tunicate CN-GM nanocomposite films and the first ever for spruce GM.
25498637	1	15	theme	enzymatic	160:168	arg1	ECN					182:184	ECN	182:184	ECN	182:184	Cellulose nanocrystals (CNs) were prepared from tunicate by enzymatic hydrolysis (ECN), TEMPO-mediated oxidation (TCN) and acid hydrolysis (ACN).
25498637	1	15	theme	enzymatic	160:168	arg1	hydrolysis					170:179	enzymatic hydrolysis	160:179	enzymatic hydrolysis (ECN)	160:185	Cellulose nanocrystals (CNs) were prepared from tunicate by enzymatic hydrolysis (ECN), TEMPO-mediated oxidation (TCN) and acid hydrolysis (ACN).
25498637	3	16	theme	Different	340:348	arg1	CNs					350:352	Different CNs	340:352	Different CNs	340:352	Different CNs were obtained with a yield of ECN>TCN>ACN with corresponding order of decreased Mw but increased crystallinity.
25498637	6	17	theme	diameter	782:789	arg1	changes					726:732	changes	726:732	changes of crystallinity, specific surface area, fibril diameter and contact angle compared with CN films	726:830	For CN-GM films, both components interacted strongly with each other, resulting in changes of crystallinity, specific surface area, fibril diameter and contact angle compared with CN films.
25498637	8	18	theme	spruce	1095:1100	arg1	GM					1102:1103	spruce GM	1095:1103	spruce GM	1095:1103	This is the first systematic study of different tunicate CN-GM nanocomposite films and the first ever for spruce GM.
25498637	0	19	theme	cellulose	9:17	arg1	nanocrystals					19:30	Tunicate cellulose nanocrystals	0:30	Tunicate cellulose nanocrystals: preparation, neat films and nanocomposite films with glucomannans.	0:98	Tunicate cellulose nanocrystals: preparation, neat films and nanocomposite films with glucomannans.
25498637	0	19	theme	cellulose	9:17	arg1	films					75:79	nanocomposite films	61:79	nanocomposite films with glucomannans	61:97	Tunicate cellulose nanocrystals: preparation, neat films and nanocomposite films with glucomannans.
25498637	0	19	theme	cellulose	9:17	arg1	preparation					33:43	preparation	33:43	preparation	33:43	Tunicate cellulose nanocrystals: preparation, neat films and nanocomposite films with glucomannans.
25498637	0	19	theme	cellulose	9:17	arg1	films					51:55	neat films	46:55	neat films	46:55	Tunicate cellulose nanocrystals: preparation, neat films and nanocomposite films with glucomannans.
25498637	6	20	theme	fibril	775:780	arg1	diameter					782:789	fibril diameter	775:789	fibril diameter	775:789	For CN-GM films, both components interacted strongly with each other, resulting in changes of crystallinity, specific surface area, fibril diameter and contact angle compared with CN films.
25498637	5	21	theme	TCN	557:559	arg1	fibrils					569:575	TCN and ACN fibrils	557:575	TCN and ACN fibrils	557:575	For CN-films, TCN and ACN fibrils were stretched and parallel to each other due to surface charges.
25498637	7	22	theme	composite	837:845	arg1	films					847:851	The composite films	833:851	The composite films	833:851	The composite films had good thermal, optical and mechanical properties; the last ones are apparently better than similar films reported in the literature.
25498637	0	23	theme	Tunicate	0:7	arg1	nanocrystals					19:30	Tunicate cellulose nanocrystals	0:30	Tunicate cellulose nanocrystals: preparation, neat films and nanocomposite films with glucomannans.	0:98	Tunicate cellulose nanocrystals: preparation, neat films and nanocomposite films with glucomannans.
25498637	0	23	theme	Tunicate	0:7	arg1	films					75:79	nanocomposite films	61:79	nanocomposite films with glucomannans	61:97	Tunicate cellulose nanocrystals: preparation, neat films and nanocomposite films with glucomannans.
25498637	0	23	theme	Tunicate	0:7	arg1	preparation					33:43	preparation	33:43	preparation	33:43	Tunicate cellulose nanocrystals: preparation, neat films and nanocomposite films with glucomannans.
25498637	0	23	theme	Tunicate	0:7	arg1	films					51:55	neat films	46:55	neat films	46:55	Tunicate cellulose nanocrystals: preparation, neat films and nanocomposite films with glucomannans.
25498637	7	24	theme	similar	947:953	arg1	films					955:959	similar films	947:959	similar films reported in the literature	947:986	The composite films had good thermal, optical and mechanical properties; the last ones are apparently better than similar films reported in the literature.
25498637	8	25	theme	nanocomposite	1052:1064	arg1	films					1066:1070	different tunicate CN-GM nanocomposite films	1027:1070	different tunicate CN-GM nanocomposite films	1027:1070	This is the first systematic study of different tunicate CN-GM nanocomposite films and the first ever for spruce GM.
25498637	2	26	from	konjac	305:310	arg1	GM					296:297	GM	296:297	GM	296:297	They were cast alone or blended with glucomannan (GM) from konjac or spruce to prepare films.
25498637	2	26	from	konjac	305:310	arg1	glucomannan					283:293	glucomannan	283:293	glucomannan (GM) from konjac or spruce to prepare films	283:337	They were cast alone or blended with glucomannan (GM) from konjac or spruce to prepare films.
25498637	1	27	theme	Cellulose	100:108	arg1	CNs					124:126	CNs	124:126	CNs	124:126	Cellulose nanocrystals (CNs) were prepared from tunicate by enzymatic hydrolysis (ECN), TEMPO-mediated oxidation (TCN) and acid hydrolysis (ACN).
25498637	1	27	theme	Cellulose	100:108	arg1	nanocrystals					110:121	Cellulose nanocrystals	100:121	Cellulose nanocrystals (CNs)	100:127	Cellulose nanocrystals (CNs) were prepared from tunicate by enzymatic hydrolysis (ECN), TEMPO-mediated oxidation (TCN) and acid hydrolysis (ACN).
25498637	4	28	theme	nanometre	497:505	arg1	scale					507:511	the nanometre scale	493:511	the nanometre scale	493:511	The CNs' diameters were on the nanometre scale, with lengths of ECN>TCN>ACN.
25498637	3	29	theme	corresponding	401:413	arg1	order					415:419	corresponding order	401:419	corresponding order of decreased Mw but increased crystallinity	401:463	Different CNs were obtained with a yield of ECN>TCN>ACN with corresponding order of decreased Mw but increased crystallinity.
25498637	7	30	contain	had	853:855	arg2	properties					894:903	good thermal, optical and mechanical properties	857:903	good thermal, optical and mechanical properties; the last ones are apparently better than similar films reported in the literature	857:986	The composite films had good thermal, optical and mechanical properties; the last ones are apparently better than similar films reported in the literature.
25498637	7	30	contain	had	853:855	arg1	films					847:851	The composite films	833:851	The composite films	833:851	The composite films had good thermal, optical and mechanical properties; the last ones are apparently better than similar films reported in the literature.
25498637	6	31	theme	area	769:772	arg1	changes					726:732	changes	726:732	changes of crystallinity, specific surface area, fibril diameter and contact angle compared with CN films	726:830	For CN-GM films, both components interacted strongly with each other, resulting in changes of crystallinity, specific surface area, fibril diameter and contact angle compared with CN films.
25498637	1	32	theme	TEMPO-mediated	188:201	arg1	TCN					214:216	TCN	214:216	TCN	214:216	Cellulose nanocrystals (CNs) were prepared from tunicate by enzymatic hydrolysis (ECN), TEMPO-mediated oxidation (TCN) and acid hydrolysis (ACN).
25498637	1	32	theme	TEMPO-mediated	188:201	arg1	oxidation					203:211	TEMPO-mediated oxidation	188:211	TEMPO-mediated oxidation (TCN)	188:217	Cellulose nanocrystals (CNs) were prepared from tunicate by enzymatic hydrolysis (ECN), TEMPO-mediated oxidation (TCN) and acid hydrolysis (ACN).
25498637	5	33	theme	surface	626:632	arg1	charges					634:640	surface charges	626:640	surface charges	626:640	For CN-films, TCN and ACN fibrils were stretched and parallel to each other due to surface charges.
25498637	6	34	theme	surface	761:767	arg1	area					769:772	specific surface area	752:772	specific surface area	752:772	For CN-GM films, both components interacted strongly with each other, resulting in changes of crystallinity, specific surface area, fibril diameter and contact angle compared with CN films.
25498637	3	35	theme	ECN>TCN>ACN	384:394	arg1	yield					375:379	a yield	373:379	a yield of ECN>TCN>ACN with corresponding order of decreased Mw but increased crystallinity	373:463	Different CNs were obtained with a yield of ECN>TCN>ACN with corresponding order of decreased Mw but increased crystallinity.
25498637	6	36	theme	specific	752:759	arg1	area					769:772	specific surface area	752:772	specific surface area	752:772	For CN-GM films, both components interacted strongly with each other, resulting in changes of crystallinity, specific surface area, fibril diameter and contact angle compared with CN films.
25498637	2	37	from	spruce	315:320	arg1	GM					296:297	GM	296:297	GM	296:297	They were cast alone or blended with glucomannan (GM) from konjac or spruce to prepare films.
25498637	2	37	from	spruce	315:320	arg1	glucomannan					283:293	glucomannan	283:293	glucomannan (GM) from konjac or spruce to prepare films	283:337	They were cast alone or blended with glucomannan (GM) from konjac or spruce to prepare films.
25498637	3	38	theme	decreased	424:432	arg1	Mw					434:435	decreased Mw	424:435	decreased Mw	424:435	Different CNs were obtained with a yield of ECN>TCN>ACN with corresponding order of decreased Mw but increased crystallinity.
25498637	0	39	theme	neat	46:49	arg1	nanocrystals					19:30	Tunicate cellulose nanocrystals	0:30	Tunicate cellulose nanocrystals: preparation, neat films and nanocomposite films with glucomannans.	0:98	Tunicate cellulose nanocrystals: preparation, neat films and nanocomposite films with glucomannans.
25498637	0	39	theme	neat	46:49	arg1	films					51:55	neat films	46:55	neat films	46:55	Tunicate cellulose nanocrystals: preparation, neat films and nanocomposite films with glucomannans.
25498637	3	40	theme	Mw	434:435	arg1	order					415:419	corresponding order	401:419	corresponding order of decreased Mw but increased crystallinity	401:463	Different CNs were obtained with a yield of ECN>TCN>ACN with corresponding order of decreased Mw but increased crystallinity.
25498637	8	41	theme	first	1001:1005	arg1	study					1018:1022	the first systematic study	997:1022	the first systematic study of different tunicate CN-GM nanocomposite films	997:1070	This is the first systematic study of different tunicate CN-GM nanocomposite films and the first ever for spruce GM.
25498637	8	41	theme	first	1001:1005	arg1	This					989:992	This	989:992	This	989:992	This is the first systematic study of different tunicate CN-GM nanocomposite films and the first ever for spruce GM.
25498637	6	42	theme	crystallinity	737:749	arg1	changes					726:732	changes	726:732	changes of crystallinity, specific surface area, fibril diameter and contact angle compared with CN films	726:830	For CN-GM films, both components interacted strongly with each other, resulting in changes of crystallinity, specific surface area, fibril diameter and contact angle compared with CN films.
25498637	7	43	theme	thermal	862:868	arg1	properties					894:903	good thermal, optical and mechanical properties	857:903	good thermal, optical and mechanical properties; the last ones are apparently better than similar films reported in the literature	857:986	The composite films had good thermal, optical and mechanical properties; the last ones are apparently better than similar films reported in the literature.
25498637	4	44	theme	ECN>TCN>ACN	530:540	arg1	lengths					519:525	lengths	519:525	lengths of ECN>TCN>ACN	519:540	The CNs' diameters were on the nanometre scale, with lengths of ECN>TCN>ACN.
25498637	3	45	with	yield	375:379	arg1	order					415:419	corresponding order	401:419	corresponding order of decreased Mw but increased crystallinity	401:463	Different CNs were obtained with a yield of ECN>TCN>ACN with corresponding order of decreased Mw but increased crystallinity.
25498637	0	46	dep	nanocrystals	19:30	arg1	films					75:79	nanocomposite films	61:79	nanocomposite films with glucomannans	61:97	Tunicate cellulose nanocrystals: preparation, neat films and nanocomposite films with glucomannans.
25498637	0	46	dep	nanocrystals	19:30	arg1	nanocrystals					19:30	Tunicate cellulose nanocrystals	0:30	Tunicate cellulose nanocrystals: preparation, neat films and nanocomposite films with glucomannans.	0:98	Tunicate cellulose nanocrystals: preparation, neat films and nanocomposite films with glucomannans.
25498637	0	46	dep	nanocrystals	19:30	arg1	preparation					33:43	preparation	33:43	preparation	33:43	Tunicate cellulose nanocrystals: preparation, neat films and nanocomposite films with glucomannans.
25498637	0	46	dep	nanocrystals	19:30	arg1	films					51:55	neat films	46:55	neat films	46:55	Tunicate cellulose nanocrystals: preparation, neat films and nanocomposite films with glucomannans.
25498637	0	47	with	films	51:55	arg1	glucomannans					86:97	glucomannans	86:97	glucomannans	86:97	Tunicate cellulose nanocrystals: preparation, neat films and nanocomposite films with glucomannans.
25498637	0	48	theme	nanocomposite	61:73	arg1	films					75:79	nanocomposite films	61:79	nanocomposite films with glucomannans	61:97	Tunicate cellulose nanocrystals: preparation, neat films and nanocomposite films with glucomannans.
25498637	0	48	theme	nanocomposite	61:73	arg1	nanocrystals					19:30	Tunicate cellulose nanocrystals	0:30	Tunicate cellulose nanocrystals: preparation, neat films and nanocomposite films with glucomannans.	0:98	Tunicate cellulose nanocrystals: preparation, neat films and nanocomposite films with glucomannans.
25498637	7	49	theme	mechanical	883:892	arg1	properties					894:903	good thermal, optical and mechanical properties	857:903	good thermal, optical and mechanical properties; the last ones are apparently better than similar films reported in the literature	857:986	The composite films had good thermal, optical and mechanical properties; the last ones are apparently better than similar films reported in the literature.
25498637	3	50	theme	crystallinity	451:463	arg1	order					415:419	corresponding order	401:419	corresponding order of decreased Mw but increased crystallinity	401:463	Different CNs were obtained with a yield of ECN>TCN>ACN with corresponding order of decreased Mw but increased crystallinity.
25498637	8	51	theme	systematic	1007:1016	arg1	study					1018:1022	the first systematic study	997:1022	the first systematic study of different tunicate CN-GM nanocomposite films	997:1070	This is the first systematic study of different tunicate CN-GM nanocomposite films and the first ever for spruce GM.
25498637	8	51	theme	systematic	1007:1016	arg1	This					989:992	This	989:992	This	989:992	This is the first systematic study of different tunicate CN-GM nanocomposite films and the first ever for spruce GM.
25498637	6	52	theme	CN	823:824	arg1	films					826:830	CN films	823:830	CN films	823:830	For CN-GM films, both components interacted strongly with each other, resulting in changes of crystallinity, specific surface area, fibril diameter and contact angle compared with CN films.
25498637	4	53	with	scale	507:511	arg1	lengths					519:525	lengths	519:525	lengths of ECN>TCN>ACN	519:540	The CNs' diameters were on the nanometre scale, with lengths of ECN>TCN>ACN.
25498637	8	54	theme	tunicate	1037:1044	arg1	films					1066:1070	different tunicate CN-GM nanocomposite films	1027:1070	different tunicate CN-GM nanocomposite films	1027:1070	This is the first systematic study of different tunicate CN-GM nanocomposite films and the first ever for spruce GM.
27432012	8	0	theme	composite	1150:1158	arg1	constructs					1160:1169	the composite constructs	1146:1169	the composite constructs	1146:1169	Cells were distributed homogeneously within the hydrogel composite, and no differences were found in cell viability between ADA-GEL and the composite constructs, proving that the addition of BGNPs did not influence cell fate.
27432012	0	1	theme	glass	95:99	arg1	nanoparticles					101:113	bioactive glass nanoparticles	85:113	bioactive glass nanoparticles	85:113	Bioplotting of a bioactive alginate dialdehyde-gelatin composite hydrogel containing bioactive glass nanoparticles.
27432012	6	2	theme	high	846:849	arg1	ibuprofen					862:870	high efficiency ibuprofen	846:870	high efficiency ibuprofen	846:870	Moreover, the composite constructs could incorporate with high efficiency ibuprofen as a drug model.
27432012	8	3	theme	BGNPs	1201:1205	arg1	addition					1189:1196	the addition	1185:1196	the addition of BGNPs	1185:1205	Cells were distributed homogeneously within the hydrogel composite, and no differences were found in cell viability between ADA-GEL and the composite constructs, proving that the addition of BGNPs did not influence cell fate.
27432012	6	4	theme	composite	802:810	arg1	constructs					812:821	the composite constructs	798:821	the composite constructs	798:821	Moreover, the composite constructs could incorporate with high efficiency ibuprofen as a drug model.
27432012	5	5	theme	bone-like	688:696	arg1	layer					706:710	bone-like apatite layer	688:710	bone-like apatite layer	688:710	EDS analysis suggested that the BGNPs loading promoted the growth of bone-like apatite layer on the surface when the constructs were immersed in a simulated body fluid.
27432012	5	6	theme	apatite	698:704	arg1	layer					706:710	bone-like apatite layer	688:710	bone-like apatite layer	688:710	EDS analysis suggested that the BGNPs loading promoted the growth of bone-like apatite layer on the surface when the constructs were immersed in a simulated body fluid.
27432012	1	7	theme	ADA-GEL	145:151	arg1	constructs					154:163	Alginate dialdehyde-gelatin (ADA-GEL) constructs	116:163	Alginate dialdehyde-gelatin (ADA-GEL) constructs incorporating bioactive glass nanoparticles (BGNPs)	116:215	Alginate dialdehyde-gelatin (ADA-GEL) constructs incorporating bioactive glass nanoparticles (BGNPs) were produced by biofabrication to obtain a grid-like highly-hydrated composite.
27432012	5	8	theme	BGNPs	651:655	arg1	loading					657:663	the BGNPs loading	647:663	the BGNPs loading	647:663	EDS analysis suggested that the BGNPs loading promoted the growth of bone-like apatite layer on the surface when the constructs were immersed in a simulated body fluid.
27432012	7	9	theme	composite	990:998	arg1	material					1000:1007	the composite material	986:1007	the composite material	986:1007	Furthermore, the biofabrication process allowed the successful incorporation of MG-63 cells into the composite material.
27432012	2	10	dep	cell	418:421	arg1	attachment					423:432	attachment	423:432	attachment	423:432	The material could induce the deposition of an apatite layer upon immersion in a biological-like environment to sustain cell attachment and proliferation.
27432012	5	11	theme	simulated	766:774	arg1	fluid					781:785	a simulated body fluid	764:785	a simulated body fluid	764:785	EDS analysis suggested that the BGNPs loading promoted the growth of bone-like apatite layer on the surface when the constructs were immersed in a simulated body fluid.
27432012	4	12	dep	doped	591:595	arg1	Strontium					581:589	Strontium	581:589	Strontium	581:589	Strontium doped BGNPs were also used.
27432012	7	13	theme	cells	975:979	arg1	incorporation					952:964	the successful incorporation	937:964	the successful incorporation of MG-63 cells into the composite material	937:1007	Furthermore, the biofabrication process allowed the successful incorporation of MG-63 cells into the composite material.
27432012	3	14	theme	BGNPs	513:517	arg1	concentrations					495:508	different concentrations	485:508	different concentrations of BGNPs synthetized from a sol-gel route, namely 0.1% and 0.5% (w/v)	485:578	Composites were formulated with different concentrations of BGNPs synthetized from a sol-gel route, namely 0.1% and 0.5% (w/v).
27432012	8	15	theme	cell	1225:1228	arg1	fate					1230:1233	cell fate	1225:1233	cell fate	1225:1233	Cells were distributed homogeneously within the hydrogel composite, and no differences were found in cell viability between ADA-GEL and the composite constructs, proving that the addition of BGNPs did not influence cell fate.
27432012	9	16	from	applications	1296:1307	arg1	engineering					1324:1334	bone tissue engineering	1312:1334	bone tissue engineering	1312:1334	Overall, the composite material showed potential for future applications in bone tissue engineering.
27432012	8	17	theme	cell	1111:1114	arg1	viability					1116:1124	cell viability	1111:1124	cell viability	1111:1124	Cells were distributed homogeneously within the hydrogel composite, and no differences were found in cell viability between ADA-GEL and the composite constructs, proving that the addition of BGNPs did not influence cell fate.
27432012	5	18	theme	body	776:779	arg1	fluid					781:785	a simulated body fluid	764:785	a simulated body fluid	764:785	EDS analysis suggested that the BGNPs loading promoted the growth of bone-like apatite layer on the surface when the constructs were immersed in a simulated body fluid.
27432012	3	19	theme	different	485:493	arg1	concentrations					495:508	different concentrations	485:508	different concentrations of BGNPs synthetized from a sol-gel route, namely 0.1% and 0.5% (w/v)	485:578	Composites were formulated with different concentrations of BGNPs synthetized from a sol-gel route, namely 0.1% and 0.5% (w/v).
27432012	4	20	theme	doped	591:595	arg1	BGNPs					597:601	Strontium doped BGNPs	581:601	Strontium doped BGNPs	581:601	Strontium doped BGNPs were also used.
27432012	9	21	theme	bone	1312:1315	arg1	engineering					1324:1334	bone tissue engineering	1312:1334	bone tissue engineering	1312:1334	Overall, the composite material showed potential for future applications in bone tissue engineering.
27432012	1	22	theme	Alginate	116:123	arg1	constructs					154:163	Alginate dialdehyde-gelatin (ADA-GEL) constructs	116:163	Alginate dialdehyde-gelatin (ADA-GEL) constructs incorporating bioactive glass nanoparticles (BGNPs)	116:215	Alginate dialdehyde-gelatin (ADA-GEL) constructs incorporating bioactive glass nanoparticles (BGNPs) were produced by biofabrication to obtain a grid-like highly-hydrated composite.
27432012	8	23	located	found	1102:1106	arg1	viability					1116:1124	cell viability	1111:1124	cell viability	1111:1124	Cells were distributed homogeneously within the hydrogel composite, and no differences were found in cell viability between ADA-GEL and the composite constructs, proving that the addition of BGNPs did not influence cell fate.
27432012	8	23	located	found	1102:1106	arg2	differences					1085:1095	no differences	1082:1095	no differences	1082:1095	Cells were distributed homogeneously within the hydrogel composite, and no differences were found in cell viability between ADA-GEL and the composite constructs, proving that the addition of BGNPs did not influence cell fate.
27432012	0	24	theme	bioactive	17:25	arg1	hydrogel					65:72	a bioactive alginate dialdehyde-gelatin composite hydrogel	15:72	a bioactive alginate dialdehyde-gelatin composite hydrogel	15:72	Bioplotting of a bioactive alginate dialdehyde-gelatin composite hydrogel containing bioactive glass nanoparticles.
27432012	5	25	theme	EDS	619:621	arg1	analysis					623:630	EDS analysis	619:630	EDS analysis	619:630	EDS analysis suggested that the BGNPs loading promoted the growth of bone-like apatite layer on the surface when the constructs were immersed in a simulated body fluid.
27432012	2	26	theme	apatite	345:351	arg1	layer					353:357	an apatite layer	342:357	an apatite layer	342:357	The material could induce the deposition of an apatite layer upon immersion in a biological-like environment to sustain cell attachment and proliferation.
27432012	2	27	theme	layer	353:357	arg1	deposition					328:337	the deposition	324:337	the deposition of an apatite layer	324:357	The material could induce the deposition of an apatite layer upon immersion in a biological-like environment to sustain cell attachment and proliferation.
27432012	3	28	theme	sol-gel	538:544	arg1	route					546:550	a sol-gel route	536:550	a sol-gel route	536:550	Composites were formulated with different concentrations of BGNPs synthetized from a sol-gel route, namely 0.1% and 0.5% (w/v).
27432012	0	29	theme	dialdehyde-gelatin	36:53	arg1	hydrogel					65:72	a bioactive alginate dialdehyde-gelatin composite hydrogel	15:72	a bioactive alginate dialdehyde-gelatin composite hydrogel	15:72	Bioplotting of a bioactive alginate dialdehyde-gelatin composite hydrogel containing bioactive glass nanoparticles.
27432012	0	30	theme	alginate	27:34	arg1	hydrogel					65:72	a bioactive alginate dialdehyde-gelatin composite hydrogel	15:72	a bioactive alginate dialdehyde-gelatin composite hydrogel	15:72	Bioplotting of a bioactive alginate dialdehyde-gelatin composite hydrogel containing bioactive glass nanoparticles.
27432012	8	31	theme	hydrogel	1058:1065	arg1	composite					1067:1075	the hydrogel composite	1054:1075	the hydrogel composite	1054:1075	Cells were distributed homogeneously within the hydrogel composite, and no differences were found in cell viability between ADA-GEL and the composite constructs, proving that the addition of BGNPs did not influence cell fate.
27432012	0	32	theme	hydrogel	65:72	arg1	Bioplotting					0:10	Bioplotting	0:10	Bioplotting of a bioactive alginate dialdehyde-gelatin composite hydrogel	0:72	Bioplotting of a bioactive alginate dialdehyde-gelatin composite hydrogel containing bioactive glass nanoparticles.
27432012	5	33	theme	layer	706:710	arg1	growth					678:683	the growth	674:683	the growth of bone-like apatite layer on the surface	674:725	EDS analysis suggested that the BGNPs loading promoted the growth of bone-like apatite layer on the surface when the constructs were immersed in a simulated body fluid.
27432012	6	34	theme	drug	877:880	arg1	model					882:886	a drug model	875:886	a drug model	875:886	Moreover, the composite constructs could incorporate with high efficiency ibuprofen as a drug model.
27432012	9	35	theme	composite	1249:1257	arg1	material					1259:1266	the composite material	1245:1266	the composite material	1245:1266	Overall, the composite material showed potential for future applications in bone tissue engineering.
27432012	2	36	theme	biological-like	379:393	arg1	environment					395:405	a biological-like environment	377:405	a biological-like environment	377:405	The material could induce the deposition of an apatite layer upon immersion in a biological-like environment to sustain cell attachment and proliferation.
27432012	0	37	theme	composite	55:63	arg1	hydrogel					65:72	a bioactive alginate dialdehyde-gelatin composite hydrogel	15:72	a bioactive alginate dialdehyde-gelatin composite hydrogel	15:72	Bioplotting of a bioactive alginate dialdehyde-gelatin composite hydrogel containing bioactive glass nanoparticles.
27432012	5	38	from	growth	678:683	arg1	surface					719:725	the surface	715:725	the surface	715:725	EDS analysis suggested that the BGNPs loading promoted the growth of bone-like apatite layer on the surface when the constructs were immersed in a simulated body fluid.
27432012	9	39	theme	future	1289:1294	arg1	applications					1296:1307	future applications	1289:1307	future applications in bone tissue engineering	1289:1334	Overall, the composite material showed potential for future applications in bone tissue engineering.
27432012	7	40	theme	successful	941:950	arg1	incorporation					952:964	the successful incorporation	937:964	the successful incorporation of MG-63 cells into the composite material	937:1007	Furthermore, the biofabrication process allowed the successful incorporation of MG-63 cells into the composite material.
27432012	7	41	theme	MG-63	969:973	arg1	cells					975:979	MG-63 cells	969:979	MG-63 cells	969:979	Furthermore, the biofabrication process allowed the successful incorporation of MG-63 cells into the composite material.
27432012	1	42	theme	dialdehyde-gelatin	125:142	arg1	constructs					154:163	Alginate dialdehyde-gelatin (ADA-GEL) constructs	116:163	Alginate dialdehyde-gelatin (ADA-GEL) constructs incorporating bioactive glass nanoparticles (BGNPs)	116:215	Alginate dialdehyde-gelatin (ADA-GEL) constructs incorporating bioactive glass nanoparticles (BGNPs) were produced by biofabrication to obtain a grid-like highly-hydrated composite.
27432012	4	43	used	used	613:616	arg2	BGNPs					597:601	Strontium doped BGNPs	581:601	Strontium doped BGNPs	581:601	Strontium doped BGNPs were also used.
27432012	1	44	theme	grid-like	261:269	arg1	composite					287:295	a grid-like highly-hydrated composite	259:295	a grid-like highly-hydrated composite	259:295	Alginate dialdehyde-gelatin (ADA-GEL) constructs incorporating bioactive glass nanoparticles (BGNPs) were produced by biofabrication to obtain a grid-like highly-hydrated composite.
27432012	0	45	theme	bioactive	85:93	arg1	nanoparticles					101:113	bioactive glass nanoparticles	85:113	bioactive glass nanoparticles	85:113	Bioplotting of a bioactive alginate dialdehyde-gelatin composite hydrogel containing bioactive glass nanoparticles.
27432012	6	46	theme	efficiency	851:860	arg1	ibuprofen					862:870	high efficiency ibuprofen	846:870	high efficiency ibuprofen	846:870	Moreover, the composite constructs could incorporate with high efficiency ibuprofen as a drug model.
27432012	1	47	theme	bioactive	179:187	arg1	BGNPs					210:214	BGNPs	210:214	BGNPs	210:214	Alginate dialdehyde-gelatin (ADA-GEL) constructs incorporating bioactive glass nanoparticles (BGNPs) were produced by biofabrication to obtain a grid-like highly-hydrated composite.
27432012	1	47	theme	bioactive	179:187	arg1	nanoparticles					195:207	bioactive glass nanoparticles	179:207	bioactive glass nanoparticles (BGNPs)	179:215	Alginate dialdehyde-gelatin (ADA-GEL) constructs incorporating bioactive glass nanoparticles (BGNPs) were produced by biofabrication to obtain a grid-like highly-hydrated composite.
27432012	1	48	theme	highly-hydrated	271:285	arg1	composite					287:295	a grid-like highly-hydrated composite	259:295	a grid-like highly-hydrated composite	259:295	Alginate dialdehyde-gelatin (ADA-GEL) constructs incorporating bioactive glass nanoparticles (BGNPs) were produced by biofabrication to obtain a grid-like highly-hydrated composite.
27432012	9	49	theme	tissue	1317:1322	arg1	engineering					1324:1334	bone tissue engineering	1312:1334	bone tissue engineering	1312:1334	Overall, the composite material showed potential for future applications in bone tissue engineering.
27432012	1	50	theme	glass	189:193	arg1	BGNPs					210:214	BGNPs	210:214	BGNPs	210:214	Alginate dialdehyde-gelatin (ADA-GEL) constructs incorporating bioactive glass nanoparticles (BGNPs) were produced by biofabrication to obtain a grid-like highly-hydrated composite.
27432012	1	50	theme	glass	189:193	arg1	nanoparticles					195:207	bioactive glass nanoparticles	179:207	bioactive glass nanoparticles (BGNPs)	179:215	Alginate dialdehyde-gelatin (ADA-GEL) constructs incorporating bioactive glass nanoparticles (BGNPs) were produced by biofabrication to obtain a grid-like highly-hydrated composite.
27432012	7	51	theme	biofabrication	906:919	arg1	process					921:927	the biofabrication process	902:927	the biofabrication process	902:927	Furthermore, the biofabrication process allowed the successful incorporation of MG-63 cells into the composite material.
26498298	9	0	theme	compositional	1846:1858	arg1	differences					1860:1870	compositional differences	1846:1870	compositional differences within the trait-positive and trait-negative hybrid variants	1846:1931	Significantly, for each tester set, compositional differences within the trait-positive and trait-negative hybrid variants were greater than differences between the GM and non-GM comparators.
26498298	8	1	theme	magnitudes	1608:1617	arg1	tester					1709:1714	the tester	1705:1714	the tester used in hybrid formation	1705:1739	Assessments of the magnitudes of differences and variance component analysis highlighted that growing location, and the tester used in hybrid formation, had a markedly greater effect on composition than did the GM trait.
26498298	8	1	theme	magnitudes	1608:1617	arg1	Assessments					1589:1599	Assessments	1589:1599	Assessments of the magnitudes of differences and variance component analysis highlighted that growing location	1589:1698	Assessments of the magnitudes of differences and variance component analysis highlighted that growing location, and the tester used in hybrid formation, had a markedly greater effect on composition than did the GM trait.
26498298	7	2	theme	few	1399:1401	arg1	differences					1426:1436	very few significant (p < 0.05) differences	1394:1436	very few significant (p < 0.05) differences between the paired trait-positive and trait-negative hybrids or between the conventional comparators and the trait-positive or trait-negative hybrids	1394:1586	Statistical analysis showed that within each hybrid tester set, there were very few significant (p < 0.05) differences between the paired trait-positive and trait-negative hybrids or between the conventional comparators and the trait-positive or trait-negative hybrids.
26498298	7	3	theme	hybrid	1364:1369	arg1	set					1378:1380	each hybrid tester set	1359:1380	each hybrid tester set	1359:1380	Statistical analysis showed that within each hybrid tester set, there were very few significant (p < 0.05) differences between the paired trait-positive and trait-negative hybrids or between the conventional comparators and the trait-positive or trait-negative hybrids.
26498298	9	4	theme	trait-positive	1883:1896	arg1	variants					1924:1931	the trait-positive and trait-negative hybrid variants	1879:1931	the trait-positive and trait-negative hybrid variants	1879:1931	Significantly, for each tester set, compositional differences within the trait-positive and trait-negative hybrid variants were greater than differences between the GM and non-GM comparators.
26498298	1	5	attach	derived	302:308	arg2	GM					284:285	GM	284:285	GM	284:285	In this study, we show that compositional differences in grain harvested from genetically modified (GM) maize hybrids derived from near-isogenic trait-positive and trait-negative segregant inbreds are more likely related to backcrossing practices than to the GM trait.
26498298	1	5	attach	derived	302:308	arg1	inbreds					373:379	near-isogenic trait-positive and trait-negative segregant inbreds	315:379	near-isogenic trait-positive and trait-negative segregant inbreds	315:379	In this study, we show that compositional differences in grain harvested from genetically modified (GM) maize hybrids derived from near-isogenic trait-positive and trait-negative segregant inbreds are more likely related to backcrossing practices than to the GM trait.
26498298	1	5	attach	derived	302:308	arg2	hybrids					294:300	genetically modified (GM) maize hybrids	262:300	genetically modified (GM) maize hybrids derived from near-isogenic trait-positive and trait-negative segregant inbreds	262:379	In this study, we show that compositional differences in grain harvested from genetically modified (GM) maize hybrids derived from near-isogenic trait-positive and trait-negative segregant inbreds are more likely related to backcrossing practices than to the GM trait.
26498298	2	6	theme	male	573:576	arg1	lines					578:582	four paired GM trait-positive (NK603: herbicide tolerance) and trait-negative near-isogenic inbred male lines	474:582	four paired GM trait-positive (NK603: herbicide tolerance) and trait-negative near-isogenic inbred male lines	474:582	To demonstrate this, four paired GM trait-positive (NK603: herbicide tolerance) and trait-negative near-isogenic inbred male lines were generated.
26498298	9	7	theme	trait-negative	1902:1915	arg1	variants					1924:1931	the trait-positive and trait-negative hybrid variants	1879:1931	the trait-positive and trait-negative hybrid variants	1879:1931	Significantly, for each tester set, compositional differences within the trait-positive and trait-negative hybrid variants were greater than differences between the GM and non-GM comparators.
26498298	8	8	from	effect	1765:1770	arg1	composition					1775:1785	composition	1775:1785	composition	1775:1785	Assessments of the magnitudes of differences and variance component analysis highlighted that growing location, and the tester used in hybrid formation, had a markedly greater effect on composition than did the GM trait.
26498298	2	9	theme	near-isogenic	552:564	arg1	lines					578:582	four paired GM trait-positive (NK603: herbicide tolerance) and trait-negative near-isogenic inbred male lines	474:582	four paired GM trait-positive (NK603: herbicide tolerance) and trait-negative near-isogenic inbred male lines	474:582	To demonstrate this, four paired GM trait-positive (NK603: herbicide tolerance) and trait-negative near-isogenic inbred male lines were generated.
26498298	6	10	theme	phytic	1277:1282	arg1	acid					1284:1287	phytic acid	1277:1287	phytic acid	1277:1287	Grain was harvested for compositional analysis; proximates (protein, starch, and oil), amino acids, fatty acids, minerals, tocopherols (α-, δ-, γ-), β-carotene, phytic acid, and raffinose were measured.
26498298	1	11	theme	compositional	212:224	arg1	differences					226:236	compositional differences	212:236	compositional differences in grain harvested from genetically modified (GM) maize hybrids derived from near-isogenic trait-positive and trait-negative segregant inbreds	212:379	In this study, we show that compositional differences in grain harvested from genetically modified (GM) maize hybrids derived from near-isogenic trait-positive and trait-negative segregant inbreds are more likely related to backcrossing practices than to the GM trait.
26498298	4	12	theme	non-GM	933:938	arg1	hybrids					940:946	non-GM hybrids	933:946	non-GM hybrids	933:946	The hypothesis was, that compositional variation within the hybrid variants would reflect differences associated with backcrossing practices and provide context to any observed differences between GM and non-GM hybrids.
26498298	7	13	theme	Statistical	1319:1329	arg1	analysis					1331:1338	Statistical analysis	1319:1338	Statistical analysis	1319:1338	Statistical analysis showed that within each hybrid tester set, there were very few significant (p < 0.05) differences between the paired trait-positive and trait-negative hybrids or between the conventional comparators and the trait-positive or trait-negative hybrids.
26498298	9	14	theme	greater	1938:1944	arg1	differences					1951:1961	greater than differences	1938:1961	greater than differences between the GM and non-GM comparators	1938:1999	Significantly, for each tester set, compositional differences within the trait-positive and trait-negative hybrid variants were greater than differences between the GM and non-GM comparators.
26498298	0	15	theme	conventional	147:158	arg1	practices					173:181	conventional backcrossing practices	147:181	conventional backcrossing practices	147:181	Maize hybrids derived from GM positive and negative segregant inbreds are compositionally equivalent: any observed differences are associated with conventional backcrossing practices.
26498298	5	16	theme	F1	953:954	arg1	hybrids					956:962	The F1 hybrids	949:962	The F1 hybrids	949:962	The F1 hybrids, as well as corresponding conventional comparator hybrids, were grown concurrently at four field sites across the United States during the 2013 season.
26498298	7	17	theme	trait-negative	1565:1578	arg1	hybrids					1580:1586	the trait-positive or trait-negative hybrids	1543:1586	the trait-positive or trait-negative hybrids	1543:1586	Statistical analysis showed that within each hybrid tester set, there were very few significant (p < 0.05) differences between the paired trait-positive and trait-negative hybrids or between the conventional comparators and the trait-positive or trait-negative hybrids.
26498298	10	18	theme	non-GM	2144:2149	arg1	comparators					2151:2161	non-GM comparators	2144:2161	non-GM comparators	2144:2161	Overall, GM trait insertion is not intrinsically a meaningful contributor to compositional variation, and observed differences between GM and non-GM comparators typically reflect incidental changes associated with conventional breeding practices.
26498298	1	19	theme	GM	443:444	arg1	trait					446:450	the GM trait	439:450	the GM trait	439:450	In this study, we show that compositional differences in grain harvested from genetically modified (GM) maize hybrids derived from near-isogenic trait-positive and trait-negative segregant inbreds are more likely related to backcrossing practices than to the GM trait.
26498298	11	20	from	discussions	2285:2295	arg1	relevance					2304:2312	the relevance	2300:2312	the relevance of negative segregants as comparators in GM assessments	2300:2368	These results contribute to ongoing discussions on the relevance of negative segregants as comparators in GM assessments.
26498298	7	21	theme	trait-positive	1547:1560	arg1	hybrids					1580:1586	the trait-positive or trait-negative hybrids	1543:1586	the trait-positive or trait-negative hybrids	1543:1586	Statistical analysis showed that within each hybrid tester set, there were very few significant (p < 0.05) differences between the paired trait-positive and trait-negative hybrids or between the conventional comparators and the trait-positive or trait-negative hybrids.
26498298	10	22	theme	meaningful	2053:2062	arg1	contributor					2064:2074	a meaningful contributor	2051:2074	a meaningful contributor to compositional variation	2051:2101	Overall, GM trait insertion is not intrinsically a meaningful contributor to compositional variation, and observed differences between GM and non-GM comparators typically reflect incidental changes associated with conventional breeding practices.
26498298	10	22	theme	meaningful	2053:2062	arg1	insertion					2020:2028	GM trait insertion	2011:2028	GM trait insertion	2011:2028	Overall, GM trait insertion is not intrinsically a meaningful contributor to compositional variation, and observed differences between GM and non-GM comparators typically reflect incidental changes associated with conventional breeding practices.
26498298	6	23	theme	amino	1203:1207	arg1	acids					1209:1213	amino acids	1203:1213	amino acids	1203:1213	Grain was harvested for compositional analysis; proximates (protein, starch, and oil), amino acids, fatty acids, minerals, tocopherols (α-, δ-, γ-), β-carotene, phytic acid, and raffinose were measured.
26498298	3	24	theme	different	628:636	arg1	testers					647:653	testers	647:653	testers	647:653	These were crossed with two different females (testers) to create a series of trait-positive and trait-negative hybrid variants.
26498298	3	24	theme	different	628:636	arg1	females					638:644	two different females	624:644	two different females (testers)	624:654	These were crossed with two different females (testers) to create a series of trait-positive and trait-negative hybrid variants.
26498298	10	25	theme	incidental	2181:2190	arg1	changes					2192:2198	incidental changes	2181:2198	incidental changes associated with conventional breeding practices	2181:2246	Overall, GM trait insertion is not intrinsically a meaningful contributor to compositional variation, and observed differences between GM and non-GM comparators typically reflect incidental changes associated with conventional breeding practices.
26498298	1	26	theme	segregant	363:371	arg1	inbreds					373:379	near-isogenic trait-positive and trait-negative segregant inbreds	315:379	near-isogenic trait-positive and trait-negative segregant inbreds	315:379	In this study, we show that compositional differences in grain harvested from genetically modified (GM) maize hybrids derived from near-isogenic trait-positive and trait-negative segregant inbreds are more likely related to backcrossing practices than to the GM trait.
26498298	7	27	theme	conventional	1514:1525	arg1	comparators					1527:1537	the conventional comparators	1510:1537	the conventional comparators	1510:1537	Statistical analysis showed that within each hybrid tester set, there were very few significant (p < 0.05) differences between the paired trait-positive and trait-negative hybrids or between the conventional comparators and the trait-positive or trait-negative hybrids.
26498298	10	28	theme	GM	2011:2012	arg1	contributor					2064:2074	a meaningful contributor	2051:2074	a meaningful contributor to compositional variation	2051:2101	Overall, GM trait insertion is not intrinsically a meaningful contributor to compositional variation, and observed differences between GM and non-GM comparators typically reflect incidental changes associated with conventional breeding practices.
26498298	10	28	theme	GM	2011:2012	arg1	insertion					2020:2028	GM trait insertion	2011:2028	GM trait insertion	2011:2028	Overall, GM trait insertion is not intrinsically a meaningful contributor to compositional variation, and observed differences between GM and non-GM comparators typically reflect incidental changes associated with conventional breeding practices.
26498298	5	29	theme	comparator	1003:1012	arg1	hybrids					1014:1020	corresponding conventional comparator hybrids	976:1020	corresponding conventional comparator hybrids	976:1020	The F1 hybrids, as well as corresponding conventional comparator hybrids, were grown concurrently at four field sites across the United States during the 2013 season.
26498298	10	30	theme	conventional	2216:2227	arg1	practices					2238:2246	conventional breeding practices	2216:2246	conventional breeding practices	2216:2246	Overall, GM trait insertion is not intrinsically a meaningful contributor to compositional variation, and observed differences between GM and non-GM comparators typically reflect incidental changes associated with conventional breeding practices.
26498298	8	31	theme	GM	1800:1801	arg1	trait					1803:1807	the GM trait	1796:1807	the GM trait	1796:1807	Assessments of the magnitudes of differences and variance component analysis highlighted that growing location, and the tester used in hybrid formation, had a markedly greater effect on composition than did the GM trait.
26498298	0	32	theme	Maize	0:4	arg1	hybrids					6:12	Maize hybrids	0:12	Maize hybrids derived from GM positive and negative segregant inbreds	0:68	Maize hybrids derived from GM positive and negative segregant inbreds are compositionally equivalent: any observed differences are associated with conventional backcrossing practices.
26498298	1	33	theme	maize	288:292	arg1	GM					284:285	GM	284:285	GM	284:285	In this study, we show that compositional differences in grain harvested from genetically modified (GM) maize hybrids derived from near-isogenic trait-positive and trait-negative segregant inbreds are more likely related to backcrossing practices than to the GM trait.
26498298	1	33	theme	maize	288:292	arg1	hybrids					294:300	genetically modified (GM) maize hybrids	262:300	genetically modified (GM) maize hybrids derived from near-isogenic trait-positive and trait-negative segregant inbreds	262:379	In this study, we show that compositional differences in grain harvested from genetically modified (GM) maize hybrids derived from near-isogenic trait-positive and trait-negative segregant inbreds are more likely related to backcrossing practices than to the GM trait.
26498298	7	34	theme	trait-negative	1476:1489	arg1	hybrids					1491:1497	the paired trait-positive and trait-negative hybrids	1446:1497	the paired trait-positive and trait-negative hybrids	1446:1497	Statistical analysis showed that within each hybrid tester set, there were very few significant (p < 0.05) differences between the paired trait-positive and trait-negative hybrids or between the conventional comparators and the trait-positive or trait-negative hybrids.
26498298	5	35	theme	corresponding	976:988	arg1	hybrids					1014:1020	corresponding conventional comparator hybrids	976:1020	corresponding conventional comparator hybrids	976:1020	The F1 hybrids, as well as corresponding conventional comparator hybrids, were grown concurrently at four field sites across the United States during the 2013 season.
26498298	0	36	theme	GM	27:28	arg1	inbreds					62:68	GM positive and negative segregant inbreds	27:68	GM positive and negative segregant inbreds	27:68	Maize hybrids derived from GM positive and negative segregant inbreds are compositionally equivalent: any observed differences are associated with conventional backcrossing practices.
26498298	7	37	theme	trait-positive	1457:1470	arg1	hybrids					1491:1497	the paired trait-positive and trait-negative hybrids	1446:1497	the paired trait-positive and trait-negative hybrids	1446:1497	Statistical analysis showed that within each hybrid tester set, there were very few significant (p < 0.05) differences between the paired trait-positive and trait-negative hybrids or between the conventional comparators and the trait-positive or trait-negative hybrids.
26498298	5	38	theme	field	1055:1059	arg1	sites					1061:1065	four field sites	1050:1065	four field sites across the United States	1050:1090	The F1 hybrids, as well as corresponding conventional comparator hybrids, were grown concurrently at four field sites across the United States during the 2013 season.
26498298	7	39	dep	significant	1403:1413	arg1	<					1418:1418	p < 0.05	1416:1423	p < 0.05	1416:1423	Statistical analysis showed that within each hybrid tester set, there were very few significant (p < 0.05) differences between the paired trait-positive and trait-negative hybrids or between the conventional comparators and the trait-positive or trait-negative hybrids.
26498298	2	40	dep	trait-positive	489:502	arg1	tolerance					522:530	NK603: herbicide tolerance	505:530	NK603: herbicide tolerance	505:530	To demonstrate this, four paired GM trait-positive (NK603: herbicide tolerance) and trait-negative near-isogenic inbred male lines were generated.
26498298	3	41	theme	trait-positive	678:691	arg1	variants					719:726	trait-positive and trait-negative hybrid variants	678:726	trait-positive and trait-negative hybrid variants	678:726	These were crossed with two different females (testers) to create a series of trait-positive and trait-negative hybrid variants.
26498298	6	42	theme	compositional	1140:1152	arg1	analysis					1154:1161	compositional analysis	1140:1161	compositional analysis	1140:1161	Grain was harvested for compositional analysis; proximates (protein, starch, and oil), amino acids, fatty acids, minerals, tocopherols (α-, δ-, γ-), β-carotene, phytic acid, and raffinose were measured.
26498298	6	43	dep	proximates	1164:1173	arg1	starch					1185:1190	starch	1185:1190	starch	1185:1190	Grain was harvested for compositional analysis; proximates (protein, starch, and oil), amino acids, fatty acids, minerals, tocopherols (α-, δ-, γ-), β-carotene, phytic acid, and raffinose were measured.
26498298	6	43	dep	proximates	1164:1173	arg1	oil					1197:1199	oil	1197:1199	oil	1197:1199	Grain was harvested for compositional analysis; proximates (protein, starch, and oil), amino acids, fatty acids, minerals, tocopherols (α-, δ-, γ-), β-carotene, phytic acid, and raffinose were measured.
26498298	6	43	dep	proximates	1164:1173	arg1	protein					1176:1182	protein	1176:1182	protein	1176:1182	Grain was harvested for compositional analysis; proximates (protein, starch, and oil), amino acids, fatty acids, minerals, tocopherols (α-, δ-, γ-), β-carotene, phytic acid, and raffinose were measured.
26498298	6	43	dep	proximates	1164:1173	arg1	proximates					1164:1173	proximates	1164:1173	proximates (protein, starch, and oil)	1164:1200	Grain was harvested for compositional analysis; proximates (protein, starch, and oil), amino acids, fatty acids, minerals, tocopherols (α-, δ-, γ-), β-carotene, phytic acid, and raffinose were measured.
26498298	9	44	theme	tester	1834:1839	arg1	set					1841:1843	each tester set	1829:1843	each tester set	1829:1843	Significantly, for each tester set, compositional differences within the trait-positive and trait-negative hybrid variants were greater than differences between the GM and non-GM comparators.
26498298	3	45	theme	variants	719:726	arg1	series					668:673	a series	666:673	a series of trait-positive and trait-negative hybrid variants	666:726	These were crossed with two different females (testers) to create a series of trait-positive and trait-negative hybrid variants.
26498298	8	46	theme	component	1647:1655	arg1	analysis					1657:1664	variance component analysis	1638:1664	variance component analysis	1638:1664	Assessments of the magnitudes of differences and variance component analysis highlighted that growing location, and the tester used in hybrid formation, had a markedly greater effect on composition than did the GM trait.
26498298	7	47	theme	p	1416:1416	arg1	<					1418:1418	p < 0.05	1416:1423	p < 0.05	1416:1423	Statistical analysis showed that within each hybrid tester set, there were very few significant (p < 0.05) differences between the paired trait-positive and trait-negative hybrids or between the conventional comparators and the trait-positive or trait-negative hybrids.
26498298	4	48	theme	observed	897:904	arg1	differences					906:916	any observed differences	893:916	any observed differences between GM and non-GM hybrids	893:946	The hypothesis was, that compositional variation within the hybrid variants would reflect differences associated with backcrossing practices and provide context to any observed differences between GM and non-GM hybrids.
26498298	3	49	theme	trait-negative	697:710	arg1	variants					719:726	trait-positive and trait-negative hybrid variants	678:726	trait-positive and trait-negative hybrid variants	678:726	These were crossed with two different females (testers) to create a series of trait-positive and trait-negative hybrid variants.
26498298	1	50	theme	modified	274:281	arg1	GM					284:285	GM	284:285	GM	284:285	In this study, we show that compositional differences in grain harvested from genetically modified (GM) maize hybrids derived from near-isogenic trait-positive and trait-negative segregant inbreds are more likely related to backcrossing practices than to the GM trait.
26498298	1	50	theme	modified	274:281	arg1	hybrids					294:300	genetically modified (GM) maize hybrids	262:300	genetically modified (GM) maize hybrids derived from near-isogenic trait-positive and trait-negative segregant inbreds	262:379	In this study, we show that compositional differences in grain harvested from genetically modified (GM) maize hybrids derived from near-isogenic trait-positive and trait-negative segregant inbreds are more likely related to backcrossing practices than to the GM trait.
26498298	7	51	theme	significant	1403:1413	arg1	differences					1426:1436	very few significant (p < 0.05) differences	1394:1436	very few significant (p < 0.05) differences between the paired trait-positive and trait-negative hybrids or between the conventional comparators and the trait-positive or trait-negative hybrids	1394:1586	Statistical analysis showed that within each hybrid tester set, there were very few significant (p < 0.05) differences between the paired trait-positive and trait-negative hybrids or between the conventional comparators and the trait-positive or trait-negative hybrids.
26498298	0	52	attach	derived	14:20	arg1	inbreds					62:68	GM positive and negative segregant inbreds	27:68	GM positive and negative segregant inbreds	27:68	Maize hybrids derived from GM positive and negative segregant inbreds are compositionally equivalent: any observed differences are associated with conventional backcrossing practices.
26498298	0	52	attach	derived	14:20	arg2	hybrids					6:12	Maize hybrids	0:12	Maize hybrids derived from GM positive and negative segregant inbreds	0:68	Maize hybrids derived from GM positive and negative segregant inbreds are compositionally equivalent: any observed differences are associated with conventional backcrossing practices.
26498298	10	53	theme	observed	2108:2115	arg1	differences					2117:2127	observed differences	2108:2127	observed differences between GM and non-GM comparators	2108:2161	Overall, GM trait insertion is not intrinsically a meaningful contributor to compositional variation, and observed differences between GM and non-GM comparators typically reflect incidental changes associated with conventional breeding practices.
26498298	8	54	theme	growing	1683:1689	arg1	location					1691:1698	that growing location	1678:1698	that growing location	1678:1698	Assessments of the magnitudes of differences and variance component analysis highlighted that growing location, and the tester used in hybrid formation, had a markedly greater effect on composition than did the GM trait.
26498298	11	55	theme	ongoing	2277:2283	arg1	discussions					2285:2295	ongoing discussions	2277:2295	ongoing discussions on the relevance of negative segregants as comparators in GM assessments	2277:2368	These results contribute to ongoing discussions on the relevance of negative segregants as comparators in GM assessments.
26498298	1	56	theme	near-isogenic	315:327	arg1	inbreds					373:379	near-isogenic trait-positive and trait-negative segregant inbreds	315:379	near-isogenic trait-positive and trait-negative segregant inbreds	315:379	In this study, we show that compositional differences in grain harvested from genetically modified (GM) maize hybrids derived from near-isogenic trait-positive and trait-negative segregant inbreds are more likely related to backcrossing practices than to the GM trait.
26498298	7	57	theme	tester	1371:1376	arg1	set					1378:1380	each hybrid tester set	1359:1380	each hybrid tester set	1359:1380	Statistical analysis showed that within each hybrid tester set, there were very few significant (p < 0.05) differences between the paired trait-positive and trait-negative hybrids or between the conventional comparators and the trait-positive or trait-negative hybrids.
26498298	8	58	dep	did	1792:1794	arg1	than					1787:1790	than	1787:1790	than	1787:1790	Assessments of the magnitudes of differences and variance component analysis highlighted that growing location, and the tester used in hybrid formation, had a markedly greater effect on composition than did the GM trait.
26498298	11	59	from	comparators	2340:2350	arg1	assessments					2358:2368	GM assessments	2355:2368	GM assessments	2355:2368	These results contribute to ongoing discussions on the relevance of negative segregants as comparators in GM assessments.
26498298	2	60	theme	inbred	566:571	arg1	lines					578:582	four paired GM trait-positive (NK603: herbicide tolerance) and trait-negative near-isogenic inbred male lines	474:582	four paired GM trait-positive (NK603: herbicide tolerance) and trait-negative near-isogenic inbred male lines	474:582	To demonstrate this, four paired GM trait-positive (NK603: herbicide tolerance) and trait-negative near-isogenic inbred male lines were generated.
26498298	9	61	theme	hybrid	1917:1922	arg1	variants					1924:1931	the trait-positive and trait-negative hybrid variants	1879:1931	the trait-positive and trait-negative hybrid variants	1879:1931	Significantly, for each tester set, compositional differences within the trait-positive and trait-negative hybrid variants were greater than differences between the GM and non-GM comparators.
26498298	11	62	theme	negative	2317:2324	arg1	segregants					2326:2335	negative segregants	2317:2335	negative segregants	2317:2335	These results contribute to ongoing discussions on the relevance of negative segregants as comparators in GM assessments.
26498298	5	63	theme	2013	1103:1106	arg1	season					1108:1113	the 2013 season	1099:1113	the 2013 season	1099:1113	The F1 hybrids, as well as corresponding conventional comparator hybrids, were grown concurrently at four field sites across the United States during the 2013 season.
26498298	2	64	theme	trait-negative	537:550	arg1	lines					578:582	four paired GM trait-positive (NK603: herbicide tolerance) and trait-negative near-isogenic inbred male lines	474:582	four paired GM trait-positive (NK603: herbicide tolerance) and trait-negative near-isogenic inbred male lines	474:582	To demonstrate this, four paired GM trait-positive (NK603: herbicide tolerance) and trait-negative near-isogenic inbred male lines were generated.
26498298	0	65	theme	backcrossing	160:171	arg1	practices					173:181	conventional backcrossing practices	147:181	conventional backcrossing practices	147:181	Maize hybrids derived from GM positive and negative segregant inbreds are compositionally equivalent: any observed differences are associated with conventional backcrossing practices.
26498298	8	66	theme	hybrid	1724:1729	arg1	formation					1731:1739	hybrid formation	1724:1739	hybrid formation	1724:1739	Assessments of the magnitudes of differences and variance component analysis highlighted that growing location, and the tester used in hybrid formation, had a markedly greater effect on composition than did the GM trait.
26498298	6	67	theme	fatty	1216:1220	arg1	acids					1222:1226	fatty acids	1216:1226	fatty acids	1216:1226	Grain was harvested for compositional analysis; proximates (protein, starch, and oil), amino acids, fatty acids, minerals, tocopherols (α-, δ-, γ-), β-carotene, phytic acid, and raffinose were measured.
26498298	9	68	theme	than	1946:1949	arg1	differences					1951:1961	greater than differences	1938:1961	greater than differences between the GM and non-GM comparators	1938:1999	Significantly, for each tester set, compositional differences within the trait-positive and trait-negative hybrid variants were greater than differences between the GM and non-GM comparators.
26498298	1	69	from	differences	226:236	arg1	grain					241:245	grain	241:245	grain harvested from genetically modified (GM) maize hybrids derived from near-isogenic trait-positive and trait-negative segregant inbreds	241:379	In this study, we show that compositional differences in grain harvested from genetically modified (GM) maize hybrids derived from near-isogenic trait-positive and trait-negative segregant inbreds are more likely related to backcrossing practices than to the GM trait.
26498298	5	70	theme	United	1078:1083	arg1	States					1085:1090	the United States	1074:1090	the United States	1074:1090	The F1 hybrids, as well as corresponding conventional comparator hybrids, were grown concurrently at four field sites across the United States during the 2013 season.
26498298	11	71	theme	segregants	2326:2335	arg1	relevance					2304:2312	the relevance	2300:2312	the relevance of negative segregants as comparators in GM assessments	2300:2368	These results contribute to ongoing discussions on the relevance of negative segregants as comparators in GM assessments.
26498298	9	72	theme	GM	1975:1976	arg1	comparators					1989:1999	the GM and non-GM comparators	1971:1999	comparators	1989:1999	Significantly, for each tester set, compositional differences within the trait-positive and trait-negative hybrid variants were greater than differences between the GM and non-GM comparators.
26498298	1	73	theme	trait-negative	348:361	arg1	inbreds					373:379	near-isogenic trait-positive and trait-negative segregant inbreds	315:379	near-isogenic trait-positive and trait-negative segregant inbreds	315:379	In this study, we show that compositional differences in grain harvested from genetically modified (GM) maize hybrids derived from near-isogenic trait-positive and trait-negative segregant inbreds are more likely related to backcrossing practices than to the GM trait.
26498298	8	74	theme	greater	1757:1763	arg1	effect					1765:1770	a markedly greater effect	1746:1770	a markedly greater effect on composition	1746:1785	Assessments of the magnitudes of differences and variance component analysis highlighted that growing location, and the tester used in hybrid formation, had a markedly greater effect on composition than did the GM trait.
26498298	2	75	theme	trait-positive	489:502	arg1	lines					578:582	four paired GM trait-positive (NK603: herbicide tolerance) and trait-negative near-isogenic inbred male lines	474:582	four paired GM trait-positive (NK603: herbicide tolerance) and trait-negative near-isogenic inbred male lines	474:582	To demonstrate this, four paired GM trait-positive (NK603: herbicide tolerance) and trait-negative near-isogenic inbred male lines were generated.
26498298	9	76	theme	non-GM	1982:1987	arg1	comparators					1989:1999	the GM and non-GM comparators	1971:1999	comparators	1989:1999	Significantly, for each tester set, compositional differences within the trait-positive and trait-negative hybrid variants were greater than differences between the GM and non-GM comparators.
26498298	10	77	theme	trait	2014:2018	arg1	contributor					2064:2074	a meaningful contributor	2051:2074	a meaningful contributor to compositional variation	2051:2101	Overall, GM trait insertion is not intrinsically a meaningful contributor to compositional variation, and observed differences between GM and non-GM comparators typically reflect incidental changes associated with conventional breeding practices.
26498298	10	77	theme	trait	2014:2018	arg1	insertion					2020:2028	GM trait insertion	2011:2028	GM trait insertion	2011:2028	Overall, GM trait insertion is not intrinsically a meaningful contributor to compositional variation, and observed differences between GM and non-GM comparators typically reflect incidental changes associated with conventional breeding practices.
26498298	5	78	theme	conventional	990:1001	arg1	hybrids					1014:1020	corresponding conventional comparator hybrids	976:1020	corresponding conventional comparator hybrids	976:1020	The F1 hybrids, as well as corresponding conventional comparator hybrids, were grown concurrently at four field sites across the United States during the 2013 season.
26498298	10	79	theme	breeding	2229:2236	arg1	practices					2238:2246	conventional breeding practices	2216:2246	conventional breeding practices	2216:2246	Overall, GM trait insertion is not intrinsically a meaningful contributor to compositional variation, and observed differences between GM and non-GM comparators typically reflect incidental changes associated with conventional breeding practices.
26498298	0	80	theme	observed	106:113	arg1	differences					115:125	any observed differences	102:125	any observed differences	102:125	Maize hybrids derived from GM positive and negative segregant inbreds are compositionally equivalent: any observed differences are associated with conventional backcrossing practices.
26498298	2	81	theme	herbicide	512:520	arg1	tolerance					522:530	NK603: herbicide tolerance	505:530	NK603: herbicide tolerance	505:530	To demonstrate this, four paired GM trait-positive (NK603: herbicide tolerance) and trait-negative near-isogenic inbred male lines were generated.
26498298	4	82	theme	hybrid	789:794	arg1	variants					796:803	the hybrid variants	785:803	the hybrid variants	785:803	The hypothesis was, that compositional variation within the hybrid variants would reflect differences associated with backcrossing practices and provide context to any observed differences between GM and non-GM hybrids.
26498298	1	83	theme	backcrossing	408:419	arg1	practices					421:429	backcrossing practices	408:429	backcrossing practices	408:429	In this study, we show that compositional differences in grain harvested from genetically modified (GM) maize hybrids derived from near-isogenic trait-positive and trait-negative segregant inbreds are more likely related to backcrossing practices than to the GM trait.
26498298	4	84	theme	compositional	754:766	arg1	variation					768:776	compositional variation	754:776	compositional variation within the hybrid variants	754:803	The hypothesis was, that compositional variation within the hybrid variants would reflect differences associated with backcrossing practices and provide context to any observed differences between GM and non-GM hybrids.
26498298	0	85	theme	positive	30:37	arg1	inbreds					62:68	GM positive and negative segregant inbreds	27:68	GM positive and negative segregant inbreds	27:68	Maize hybrids derived from GM positive and negative segregant inbreds are compositionally equivalent: any observed differences are associated with conventional backcrossing practices.
26498298	2	86	theme	paired	479:484	arg1	lines					578:582	four paired GM trait-positive (NK603: herbicide tolerance) and trait-negative near-isogenic inbred male lines	474:582	four paired GM trait-positive (NK603: herbicide tolerance) and trait-negative near-isogenic inbred male lines	474:582	To demonstrate this, four paired GM trait-positive (NK603: herbicide tolerance) and trait-negative near-isogenic inbred male lines were generated.
26498298	0	87	theme	negative	43:50	arg1	inbreds					62:68	GM positive and negative segregant inbreds	27:68	GM positive and negative segregant inbreds	27:68	Maize hybrids derived from GM positive and negative segregant inbreds are compositionally equivalent: any observed differences are associated with conventional backcrossing practices.
26498298	8	88	contain	had	1742:1744	arg2	effect					1765:1770	a markedly greater effect	1746:1770	a markedly greater effect on composition	1746:1785	Assessments of the magnitudes of differences and variance component analysis highlighted that growing location, and the tester used in hybrid formation, had a markedly greater effect on composition than did the GM trait.
26498298	8	88	contain	had	1742:1744	arg1	tester					1709:1714	the tester	1705:1714	the tester used in hybrid formation	1705:1739	Assessments of the magnitudes of differences and variance component analysis highlighted that growing location, and the tester used in hybrid formation, had a markedly greater effect on composition than did the GM trait.
26498298	8	88	contain	had	1742:1744	arg1	Assessments					1589:1599	Assessments	1589:1599	Assessments of the magnitudes of differences and variance component analysis highlighted that growing location	1589:1698	Assessments of the magnitudes of differences and variance component analysis highlighted that growing location, and the tester used in hybrid formation, had a markedly greater effect on composition than did the GM trait.
26498298	0	89	dep	positive	30:37	arg1	segregant					52:60	segregant	52:60	segregant	52:60	Maize hybrids derived from GM positive and negative segregant inbreds are compositionally equivalent: any observed differences are associated with conventional backcrossing practices.
26498298	8	90	theme	differences	1622:1632	arg1	magnitudes					1608:1617	the magnitudes	1604:1617	the magnitudes of differences and variance component analysis highlighted that growing location	1604:1698	Assessments of the magnitudes of differences and variance component analysis highlighted that growing location, and the tester used in hybrid formation, had a markedly greater effect on composition than did the GM trait.
26498298	1	91	theme	trait-positive	329:342	arg1	inbreds					373:379	near-isogenic trait-positive and trait-negative segregant inbreds	315:379	near-isogenic trait-positive and trait-negative segregant inbreds	315:379	In this study, we show that compositional differences in grain harvested from genetically modified (GM) maize hybrids derived from near-isogenic trait-positive and trait-negative segregant inbreds are more likely related to backcrossing practices than to the GM trait.
26498298	7	92	theme	paired	1450:1455	arg1	hybrids					1491:1497	the paired trait-positive and trait-negative hybrids	1446:1497	the paired trait-positive and trait-negative hybrids	1446:1497	Statistical analysis showed that within each hybrid tester set, there were very few significant (p < 0.05) differences between the paired trait-positive and trait-negative hybrids or between the conventional comparators and the trait-positive or trait-negative hybrids.
26498298	11	93	theme	GM	2355:2356	arg1	assessments					2358:2368	GM assessments	2355:2368	GM assessments	2355:2368	These results contribute to ongoing discussions on the relevance of negative segregants as comparators in GM assessments.
26498298	8	94	theme	variance	1638:1645	arg1	analysis					1657:1664	variance component analysis	1638:1664	variance component analysis	1638:1664	Assessments of the magnitudes of differences and variance component analysis highlighted that growing location, and the tester used in hybrid formation, had a markedly greater effect on composition than did the GM trait.
26498298	6	95	dep	tocopherols	1239:1249	arg1	γ-					1260:1261	γ-	1260:1261	γ-	1260:1261	Grain was harvested for compositional analysis; proximates (protein, starch, and oil), amino acids, fatty acids, minerals, tocopherols (α-, δ-, γ-), β-carotene, phytic acid, and raffinose were measured.
26498298	10	96	theme	compositional	2079:2091	arg1	variation					2093:2101	compositional variation	2079:2101	compositional variation	2079:2101	Overall, GM trait insertion is not intrinsically a meaningful contributor to compositional variation, and observed differences between GM and non-GM comparators typically reflect incidental changes associated with conventional breeding practices.
26498298	3	97	theme	hybrid	712:717	arg1	variants					719:726	trait-positive and trait-negative hybrid variants	678:726	trait-positive and trait-negative hybrid variants	678:726	These were crossed with two different females (testers) to create a series of trait-positive and trait-negative hybrid variants.
26498298	8	98	theme	analysis	1657:1664	arg1	magnitudes					1608:1617	the magnitudes	1604:1617	the magnitudes of differences and variance component analysis highlighted that growing location	1604:1698	Assessments of the magnitudes of differences and variance component analysis highlighted that growing location, and the tester used in hybrid formation, had a markedly greater effect on composition than did the GM trait.
25199870	5	0	theme	polyelectrolyte	926:940	arg1	formation					902:910	the formation	898:910	the formation of nanometric polyelectrolyte	898:940	The results of optimization study revealed that the formation of nanometric polyelectrolyte is favored at the median level of carboxymethylated arabinoxylan and chitosan concentration.
25199870	0	1	theme	drug	95:98	arg1	delivery					100:107	nanoparticulate drug delivery	79:107	nanoparticulate drug delivery	79:107	Psyllium arabinoxylan: carboxymethylation, characterization and evaluation for nanoparticulate drug delivery.
25199870	4	2	theme	carboxymethylated	622:638	arg1	arabinoxylan					640:651	carboxymethylated arabinoxylan	622:651	carboxymethylated arabinoxylan	622:651	Further, the effect of concentrations of carboxymethylated arabinoxylan and chitosan on the particle size and particle size distribution of ibuprofen loaded polyelectrolyte nanoparticles was screened using two-factor, three-level central composite experimental design.
25199870	5	3	dep	arabinoxylan	994:1005	arg1	concentration					1020:1032	concentration	1020:1032	concentration	1020:1032	The results of optimization study revealed that the formation of nanometric polyelectrolyte is favored at the median level of carboxymethylated arabinoxylan and chitosan concentration.
25199870	7	4	theme	complex	1287:1293	arg1	nanoparticles					1295:1307	polyelectrolyte complex nanoparticles	1271:1307	polyelectrolyte complex nanoparticles	1271:1307	Further, polyelectrolyte complex nanoparticles were found to release ibuprofen over a prolonged period of 10h following Higuchi's square root release kinetics with the mechanism of release being combination of diffusion and erosion of matrix.
25199870	4	5	theme	particle	691:698	arg1	size					700:703	particle size	691:703	particle size	691:703	Further, the effect of concentrations of carboxymethylated arabinoxylan and chitosan on the particle size and particle size distribution of ibuprofen loaded polyelectrolyte nanoparticles was screened using two-factor, three-level central composite experimental design.
25199870	7	6	theme	polyelectrolyte	1271:1285	arg1	nanoparticles					1295:1307	polyelectrolyte complex nanoparticles	1271:1307	polyelectrolyte complex nanoparticles	1271:1307	Further, polyelectrolyte complex nanoparticles were found to release ibuprofen over a prolonged period of 10h following Higuchi's square root release kinetics with the mechanism of release being combination of diffusion and erosion of matrix.
25199870	7	7	theme	erosion	1486:1492	arg1	mechanism					1430:1438	the mechanism	1426:1438	the mechanism of release	1426:1449	Further, polyelectrolyte complex nanoparticles were found to release ibuprofen over a prolonged period of 10h following Higuchi's square root release kinetics with the mechanism of release being combination of diffusion and erosion of matrix.
25199870	7	7	theme	erosion	1486:1492	arg1	combination					1457:1467	combination	1457:1467	combination of diffusion and erosion of matrix	1457:1502	Further, polyelectrolyte complex nanoparticles were found to release ibuprofen over a prolonged period of 10h following Higuchi's square root release kinetics with the mechanism of release being combination of diffusion and erosion of matrix.
25199870	3	8	theme	psyllium	461:468	arg1	arabinoxylan					470:481	psyllium arabinoxylan	461:481	psyllium arabinoxylan	461:481	Carboxymethylation of psyllium arabinoxylan was observed to increase its crystallinity, improve thermal stability and decrease the viscosity.
25199870	6	9	theme	chitosan	1100:1107	arg1	concentrations					1047:1060	The optimal concentrations	1035:1060	The optimal concentrations of carboxymethylated arabinoxylan and chitosan	1035:1107	The optimal concentrations of carboxymethylated arabinoxylan and chitosan were found to be 0.0779% (w/v) and 0.0693% (w/v) respectively, which provided polyelectrolyte particles of size 337.2 nm and polydispersity index 0.335.
25199870	6	9	theme	chitosan	1100:1107	arg1	%					1132:1132	0.0779%	1126:1132	0.0779% (w/v)	1126:1138	The optimal concentrations of carboxymethylated arabinoxylan and chitosan were found to be 0.0779% (w/v) and 0.0693% (w/v) respectively, which provided polyelectrolyte particles of size 337.2 nm and polydispersity index 0.335.
25199870	7	10	theme	square	1392:1397	arg1	kinetics					1412:1419	Higuchi's square root release kinetics	1382:1419	Higuchi's square root release kinetics	1382:1419	Further, polyelectrolyte complex nanoparticles were found to release ibuprofen over a prolonged period of 10h following Higuchi's square root release kinetics with the mechanism of release being combination of diffusion and erosion of matrix.
25199870	4	11	dep	size	682:685	arg1	distribution					705:716	distribution	705:716	distribution	705:716	Further, the effect of concentrations of carboxymethylated arabinoxylan and chitosan on the particle size and particle size distribution of ibuprofen loaded polyelectrolyte nanoparticles was screened using two-factor, three-level central composite experimental design.
25199870	4	11	dep	size	682:685	arg1	the					669:671	the	669:671	the	669:671	Further, the effect of concentrations of carboxymethylated arabinoxylan and chitosan on the particle size and particle size distribution of ibuprofen loaded polyelectrolyte nanoparticles was screened using two-factor, three-level central composite experimental design.
25199870	4	12	from	effect	594:599	arg1	size					682:685	particle size	673:685	particle size	673:685	Further, the effect of concentrations of carboxymethylated arabinoxylan and chitosan on the particle size and particle size distribution of ibuprofen loaded polyelectrolyte nanoparticles was screened using two-factor, three-level central composite experimental design.
25199870	4	12	from	effect	594:599	arg1	size					700:703	particle size	691:703	particle size	691:703	Further, the effect of concentrations of carboxymethylated arabinoxylan and chitosan on the particle size and particle size distribution of ibuprofen loaded polyelectrolyte nanoparticles was screened using two-factor, three-level central composite experimental design.
25199870	6	13	theme	size	1216:1219	arg1	nm					1227:1228	size 337.2 nm	1216:1228	size 337.2 nm	1216:1228	The optimal concentrations of carboxymethylated arabinoxylan and chitosan were found to be 0.0779% (w/v) and 0.0693% (w/v) respectively, which provided polyelectrolyte particles of size 337.2 nm and polydispersity index 0.335.
25199870	1	14	theme	carboxymethylated	189:205	arg1	arabinoxylan					216:227	carboxymethylated psyllium arabinoxylan	189:227	carboxymethylated psyllium arabinoxylan	189:227	The objective of present investigation was to optimize the interaction between carboxymethylated psyllium arabinoxylan and chitosan to prepare polyelectrolyte naoparticles for drug delivery applications.
25199870	7	15	theme	10h	1368:1370	arg1	period					1358:1363	a prolonged period	1346:1363	a prolonged period of 10h following Higuchi's square root release kinetics	1346:1419	Further, polyelectrolyte complex nanoparticles were found to release ibuprofen over a prolonged period of 10h following Higuchi's square root release kinetics with the mechanism of release being combination of diffusion and erosion of matrix.
25199870	6	16	theme	arabinoxylan	1083:1094	arg1	concentrations					1047:1060	The optimal concentrations	1035:1060	The optimal concentrations of carboxymethylated arabinoxylan and chitosan	1035:1107	The optimal concentrations of carboxymethylated arabinoxylan and chitosan were found to be 0.0779% (w/v) and 0.0693% (w/v) respectively, which provided polyelectrolyte particles of size 337.2 nm and polydispersity index 0.335.
25199870	6	16	theme	arabinoxylan	1083:1094	arg1	%					1132:1132	0.0779%	1126:1132	0.0779% (w/v)	1126:1138	The optimal concentrations of carboxymethylated arabinoxylan and chitosan were found to be 0.0779% (w/v) and 0.0693% (w/v) respectively, which provided polyelectrolyte particles of size 337.2 nm and polydispersity index 0.335.
25199870	1	17	theme	psyllium	207:214	arg1	arabinoxylan					216:227	carboxymethylated psyllium arabinoxylan	189:227	carboxymethylated psyllium arabinoxylan	189:227	The objective of present investigation was to optimize the interaction between carboxymethylated psyllium arabinoxylan and chitosan to prepare polyelectrolyte naoparticles for drug delivery applications.
25199870	7	18	theme	prolonged	1348:1356	arg1	period					1358:1363	a prolonged period	1346:1363	a prolonged period of 10h following Higuchi's square root release kinetics	1346:1419	Further, polyelectrolyte complex nanoparticles were found to release ibuprofen over a prolonged period of 10h following Higuchi's square root release kinetics with the mechanism of release being combination of diffusion and erosion of matrix.
25199870	6	19	theme	carboxymethylated	1065:1081	arg1	arabinoxylan					1083:1094	carboxymethylated arabinoxylan	1065:1094	carboxymethylated arabinoxylan	1065:1094	The optimal concentrations of carboxymethylated arabinoxylan and chitosan were found to be 0.0779% (w/v) and 0.0693% (w/v) respectively, which provided polyelectrolyte particles of size 337.2 nm and polydispersity index 0.335.
25199870	6	20	theme	index	1249:1253	arg1	particles					1203:1211	polyelectrolyte particles	1187:1211	polyelectrolyte particles of size 337.2 nm and polydispersity index 0.335	1187:1259	The optimal concentrations of carboxymethylated arabinoxylan and chitosan were found to be 0.0779% (w/v) and 0.0693% (w/v) respectively, which provided polyelectrolyte particles of size 337.2 nm and polydispersity index 0.335.
25199870	7	21	theme	root	1399:1402	arg1	kinetics					1412:1419	Higuchi's square root release kinetics	1382:1419	Higuchi's square root release kinetics	1382:1419	Further, polyelectrolyte complex nanoparticles were found to release ibuprofen over a prolonged period of 10h following Higuchi's square root release kinetics with the mechanism of release being combination of diffusion and erosion of matrix.
25199870	2	22	theme	monochloroacetic	390:405	arg1	acid					407:410	monochloroacetic acid	390:410	monochloroacetic acid	390:410	Arabinoxylan extracted from psyllium was carboxymethylated by reacting with monochloroacetic acid under alkaline conditions.
25199870	4	23	theme	composite	819:827	arg1	design					842:847	two-factor, three-level central composite experimental design	787:847	two-factor, three-level central composite experimental design	787:847	Further, the effect of concentrations of carboxymethylated arabinoxylan and chitosan on the particle size and particle size distribution of ibuprofen loaded polyelectrolyte nanoparticles was screened using two-factor, three-level central composite experimental design.
25199870	0	24	theme	Psyllium	0:7	arg1	arabinoxylan					9:20	Psyllium arabinoxylan	0:20	Psyllium arabinoxylan: carboxymethylation, characterization and evaluation for nanoparticulate drug delivery.	0:108	Psyllium arabinoxylan: carboxymethylation, characterization and evaluation for nanoparticulate drug delivery.
25199870	4	25	theme	particle	673:680	arg1	size					682:685	particle size	673:685	particle size	673:685	Further, the effect of concentrations of carboxymethylated arabinoxylan and chitosan on the particle size and particle size distribution of ibuprofen loaded polyelectrolyte nanoparticles was screened using two-factor, three-level central composite experimental design.
25199870	3	26	theme	thermal	535:541	arg1	stability					543:551	thermal stability	535:551	thermal stability	535:551	Carboxymethylation of psyllium arabinoxylan was observed to increase its crystallinity, improve thermal stability and decrease the viscosity.
25199870	3	27	theme	arabinoxylan	470:481	arg1	Carboxymethylation					439:456	Carboxymethylation	439:456	Carboxymethylation of psyllium arabinoxylan	439:481	Carboxymethylation of psyllium arabinoxylan was observed to increase its crystallinity, improve thermal stability and decrease the viscosity.
25199870	5	28	theme	arabinoxylan	994:1005	arg1	level					967:971	the median level	956:971	the median level of carboxymethylated arabinoxylan and chitosan concentration	956:1032	The results of optimization study revealed that the formation of nanometric polyelectrolyte is favored at the median level of carboxymethylated arabinoxylan and chitosan concentration.
25199870	5	29	theme	carboxymethylated	976:992	arg1	arabinoxylan					994:1005	carboxymethylated arabinoxylan	976:1005	carboxymethylated arabinoxylan	976:1005	The results of optimization study revealed that the formation of nanometric polyelectrolyte is favored at the median level of carboxymethylated arabinoxylan and chitosan concentration.
25199870	5	30	theme	chitosan	1011:1018	arg1	level					967:971	the median level	956:971	the median level of carboxymethylated arabinoxylan and chitosan concentration	956:1032	The results of optimization study revealed that the formation of nanometric polyelectrolyte is favored at the median level of carboxymethylated arabinoxylan and chitosan concentration.
25199870	2	31	theme	alkaline	418:425	arg1	conditions					427:436	alkaline conditions	418:436	alkaline conditions	418:436	Arabinoxylan extracted from psyllium was carboxymethylated by reacting with monochloroacetic acid under alkaline conditions.
25199870	5	32	theme	optimization	865:876	arg1	study					878:882	optimization study	865:882	optimization study	865:882	The results of optimization study revealed that the formation of nanometric polyelectrolyte is favored at the median level of carboxymethylated arabinoxylan and chitosan concentration.
25199870	4	33	dep	two-factor	787:796	arg1	three-level					799:809	three-level	799:809	three-level	799:809	Further, the effect of concentrations of carboxymethylated arabinoxylan and chitosan on the particle size and particle size distribution of ibuprofen loaded polyelectrolyte nanoparticles was screened using two-factor, three-level central composite experimental design.
25199870	4	34	theme	central	811:817	arg1	design					842:847	two-factor, three-level central composite experimental design	787:847	two-factor, three-level central composite experimental design	787:847	Further, the effect of concentrations of carboxymethylated arabinoxylan and chitosan on the particle size and particle size distribution of ibuprofen loaded polyelectrolyte nanoparticles was screened using two-factor, three-level central composite experimental design.
25199870	4	35	theme	two-factor	787:796	arg1	design					842:847	two-factor, three-level central composite experimental design	787:847	two-factor, three-level central composite experimental design	787:847	Further, the effect of concentrations of carboxymethylated arabinoxylan and chitosan on the particle size and particle size distribution of ibuprofen loaded polyelectrolyte nanoparticles was screened using two-factor, three-level central composite experimental design.
25199870	6	36	theme	polyelectrolyte	1187:1201	arg1	particles					1203:1211	polyelectrolyte particles	1187:1211	polyelectrolyte particles of size 337.2 nm and polydispersity index 0.335	1187:1259	The optimal concentrations of carboxymethylated arabinoxylan and chitosan were found to be 0.0779% (w/v) and 0.0693% (w/v) respectively, which provided polyelectrolyte particles of size 337.2 nm and polydispersity index 0.335.
25199870	5	37	theme	study	878:882	arg1	results					854:860	The results	850:860	The results of optimization study	850:882	The results of optimization study revealed that the formation of nanometric polyelectrolyte is favored at the median level of carboxymethylated arabinoxylan and chitosan concentration.
25199870	6	38	theme	optimal	1039:1045	arg1	concentrations					1047:1060	The optimal concentrations	1035:1060	The optimal concentrations of carboxymethylated arabinoxylan and chitosan	1035:1107	The optimal concentrations of carboxymethylated arabinoxylan and chitosan were found to be 0.0779% (w/v) and 0.0693% (w/v) respectively, which provided polyelectrolyte particles of size 337.2 nm and polydispersity index 0.335.
25199870	6	38	theme	optimal	1039:1045	arg1	%					1132:1132	0.0779%	1126:1132	0.0779% (w/v)	1126:1138	The optimal concentrations of carboxymethylated arabinoxylan and chitosan were found to be 0.0779% (w/v) and 0.0693% (w/v) respectively, which provided polyelectrolyte particles of size 337.2 nm and polydispersity index 0.335.
25199870	1	39	theme	drug	286:289	arg1	applications					300:311	drug delivery applications	286:311	drug delivery applications	286:311	The objective of present investigation was to optimize the interaction between carboxymethylated psyllium arabinoxylan and chitosan to prepare polyelectrolyte naoparticles for drug delivery applications.
25199870	1	40	theme	polyelectrolyte	253:267	arg1	naoparticles					269:280	polyelectrolyte naoparticles	253:280	polyelectrolyte naoparticles	253:280	The objective of present investigation was to optimize the interaction between carboxymethylated psyllium arabinoxylan and chitosan to prepare polyelectrolyte naoparticles for drug delivery applications.
25199870	4	41	theme	arabinoxylan	640:651	arg1	concentrations					604:617	concentrations	604:617	concentrations of carboxymethylated arabinoxylan and chitosan	604:664	Further, the effect of concentrations of carboxymethylated arabinoxylan and chitosan on the particle size and particle size distribution of ibuprofen loaded polyelectrolyte nanoparticles was screened using two-factor, three-level central composite experimental design.
25199870	7	42	theme	release	1404:1410	arg1	kinetics					1412:1419	Higuchi's square root release kinetics	1382:1419	Higuchi's square root release kinetics	1382:1419	Further, polyelectrolyte complex nanoparticles were found to release ibuprofen over a prolonged period of 10h following Higuchi's square root release kinetics with the mechanism of release being combination of diffusion and erosion of matrix.
25199870	1	43	theme	present	127:133	arg1	investigation					135:147	present investigation	127:147	present investigation	127:147	The objective of present investigation was to optimize the interaction between carboxymethylated psyllium arabinoxylan and chitosan to prepare polyelectrolyte naoparticles for drug delivery applications.
25199870	5	44	theme	median	960:965	arg1	level					967:971	the median level	956:971	the median level of carboxymethylated arabinoxylan and chitosan concentration	956:1032	The results of optimization study revealed that the formation of nanometric polyelectrolyte is favored at the median level of carboxymethylated arabinoxylan and chitosan concentration.
25199870	7	45	theme	matrix	1497:1502	arg1	erosion					1486:1492	erosion	1486:1492	erosion	1486:1492	Further, polyelectrolyte complex nanoparticles were found to release ibuprofen over a prolonged period of 10h following Higuchi's square root release kinetics with the mechanism of release being combination of diffusion and erosion of matrix.
25199870	7	45	theme	matrix	1497:1502	arg1	diffusion					1472:1480	diffusion	1472:1480	diffusion	1472:1480	Further, polyelectrolyte complex nanoparticles were found to release ibuprofen over a prolonged period of 10h following Higuchi's square root release kinetics with the mechanism of release being combination of diffusion and erosion of matrix.
25199870	1	46	theme	investigation	135:147	arg1	objective					114:122	The objective	110:122	The objective of present investigation	110:147	The objective of present investigation was to optimize the interaction between carboxymethylated psyllium arabinoxylan and chitosan to prepare polyelectrolyte naoparticles for drug delivery applications.
25199870	4	47	theme	chitosan	657:664	arg1	concentrations					604:617	concentrations	604:617	concentrations of carboxymethylated arabinoxylan and chitosan	604:664	Further, the effect of concentrations of carboxymethylated arabinoxylan and chitosan on the particle size and particle size distribution of ibuprofen loaded polyelectrolyte nanoparticles was screened using two-factor, three-level central composite experimental design.
25199870	4	48	theme	loaded	731:736	arg1	nanoparticles					754:766	ibuprofen loaded polyelectrolyte nanoparticles	721:766	ibuprofen loaded polyelectrolyte nanoparticles	721:766	Further, the effect of concentrations of carboxymethylated arabinoxylan and chitosan on the particle size and particle size distribution of ibuprofen loaded polyelectrolyte nanoparticles was screened using two-factor, three-level central composite experimental design.
25199870	4	49	dep	loaded	731:736	arg1	ibuprofen					721:729	ibuprofen	721:729	ibuprofen	721:729	Further, the effect of concentrations of carboxymethylated arabinoxylan and chitosan on the particle size and particle size distribution of ibuprofen loaded polyelectrolyte nanoparticles was screened using two-factor, three-level central composite experimental design.
25199870	6	50	theme	nm	1227:1228	arg1	particles					1203:1211	polyelectrolyte particles	1187:1211	polyelectrolyte particles of size 337.2 nm and polydispersity index 0.335	1187:1259	The optimal concentrations of carboxymethylated arabinoxylan and chitosan were found to be 0.0779% (w/v) and 0.0693% (w/v) respectively, which provided polyelectrolyte particles of size 337.2 nm and polydispersity index 0.335.
25199870	7	51	theme	release	1443:1449	arg1	mechanism					1430:1438	the mechanism	1426:1438	the mechanism of release	1426:1449	Further, polyelectrolyte complex nanoparticles were found to release ibuprofen over a prolonged period of 10h following Higuchi's square root release kinetics with the mechanism of release being combination of diffusion and erosion of matrix.
25199870	7	51	theme	release	1443:1449	arg1	combination					1457:1467	combination	1457:1467	combination of diffusion and erosion of matrix	1457:1502	Further, polyelectrolyte complex nanoparticles were found to release ibuprofen over a prolonged period of 10h following Higuchi's square root release kinetics with the mechanism of release being combination of diffusion and erosion of matrix.
25199870	0	52	theme	nanoparticulate	79:93	arg1	delivery					100:107	nanoparticulate drug delivery	79:107	nanoparticulate drug delivery	79:107	Psyllium arabinoxylan: carboxymethylation, characterization and evaluation for nanoparticulate drug delivery.
25199870	7	53	theme	diffusion	1472:1480	arg1	mechanism					1430:1438	the mechanism	1426:1438	the mechanism of release	1426:1449	Further, polyelectrolyte complex nanoparticles were found to release ibuprofen over a prolonged period of 10h following Higuchi's square root release kinetics with the mechanism of release being combination of diffusion and erosion of matrix.
25199870	7	53	theme	diffusion	1472:1480	arg1	combination					1457:1467	combination	1457:1467	combination of diffusion and erosion of matrix	1457:1502	Further, polyelectrolyte complex nanoparticles were found to release ibuprofen over a prolonged period of 10h following Higuchi's square root release kinetics with the mechanism of release being combination of diffusion and erosion of matrix.
25199870	0	54	dep	arabinoxylan	9:20	arg1	characterization					43:58	characterization	43:58	characterization	43:58	Psyllium arabinoxylan: carboxymethylation, characterization and evaluation for nanoparticulate drug delivery.
25199870	0	54	dep	arabinoxylan	9:20	arg1	carboxymethylation					23:40	carboxymethylation	23:40	carboxymethylation	23:40	Psyllium arabinoxylan: carboxymethylation, characterization and evaluation for nanoparticulate drug delivery.
25199870	0	54	dep	arabinoxylan	9:20	arg1	evaluation					64:73	evaluation	64:73	evaluation	64:73	Psyllium arabinoxylan: carboxymethylation, characterization and evaluation for nanoparticulate drug delivery.
25199870	6	55	theme	polydispersity	1234:1247	arg1	index					1249:1253	polydispersity index 0.335	1234:1259	polydispersity index 0.335	1234:1259	The optimal concentrations of carboxymethylated arabinoxylan and chitosan were found to be 0.0779% (w/v) and 0.0693% (w/v) respectively, which provided polyelectrolyte particles of size 337.2 nm and polydispersity index 0.335.
25199870	4	56	theme	nanoparticles	754:766	arg1	size					682:685	particle size	673:685	particle size	673:685	Further, the effect of concentrations of carboxymethylated arabinoxylan and chitosan on the particle size and particle size distribution of ibuprofen loaded polyelectrolyte nanoparticles was screened using two-factor, three-level central composite experimental design.
25199870	4	56	theme	nanoparticles	754:766	arg1	size					700:703	particle size	691:703	particle size	691:703	Further, the effect of concentrations of carboxymethylated arabinoxylan and chitosan on the particle size and particle size distribution of ibuprofen loaded polyelectrolyte nanoparticles was screened using two-factor, three-level central composite experimental design.
25199870	5	57	theme	nanometric	915:924	arg1	polyelectrolyte					926:940	nanometric polyelectrolyte	915:940	nanometric polyelectrolyte	915:940	The results of optimization study revealed that the formation of nanometric polyelectrolyte is favored at the median level of carboxymethylated arabinoxylan and chitosan concentration.
25199870	4	58	theme	concentrations	604:617	arg1	effect					594:599	the effect	590:599	the effect of concentrations of carboxymethylated arabinoxylan and chitosan on the particle size and particle size distribution of ibuprofen loaded polyelectrolyte nanoparticles	590:766	Further, the effect of concentrations of carboxymethylated arabinoxylan and chitosan on the particle size and particle size distribution of ibuprofen loaded polyelectrolyte nanoparticles was screened using two-factor, three-level central composite experimental design.
25199870	1	59	theme	delivery	291:298	arg1	applications					300:311	drug delivery applications	286:311	drug delivery applications	286:311	The objective of present investigation was to optimize the interaction between carboxymethylated psyllium arabinoxylan and chitosan to prepare polyelectrolyte naoparticles for drug delivery applications.
25199870	4	60	theme	experimental	829:840	arg1	design					842:847	two-factor, three-level central composite experimental design	787:847	two-factor, three-level central composite experimental design	787:847	Further, the effect of concentrations of carboxymethylated arabinoxylan and chitosan on the particle size and particle size distribution of ibuprofen loaded polyelectrolyte nanoparticles was screened using two-factor, three-level central composite experimental design.
25199870	4	61	theme	polyelectrolyte	738:752	arg1	nanoparticles					754:766	ibuprofen loaded polyelectrolyte nanoparticles	721:766	ibuprofen loaded polyelectrolyte nanoparticles	721:766	Further, the effect of concentrations of carboxymethylated arabinoxylan and chitosan on the particle size and particle size distribution of ibuprofen loaded polyelectrolyte nanoparticles was screened using two-factor, three-level central composite experimental design.
26590895	4	0	theme	best	503:506	arg1	BCI					530:532	BCI	530:532	BCI	530:532	Results showed that the best blood-clotting index (BCI) was achieved in vitro by a chitosan/gelatin sponge (CG) ratio of 5/5 (W/W).
26590895	4	0	theme	best	503:506	arg1	index					523:527	the best blood-clotting index	499:527	the best blood-clotting index (BCI)	499:533	Results showed that the best blood-clotting index (BCI) was achieved in vitro by a chitosan/gelatin sponge (CG) ratio of 5/5 (W/W).
26590895	2	1	dep	silkworm	279:286	arg1	pupae					288:292	pupae	288:292	pupae	288:292	The purpose of this study was to test the hemostatic properties of a chitosan composite obtained from silkworm pupae and gelatin.
26590895	4	2	theme	sponge	579:584	arg1	ratio					591:595	a chitosan/gelatin sponge (CG) ratio	560:595	a chitosan/gelatin sponge (CG) ratio of 5/5 (W/W)	560:608	Results showed that the best blood-clotting index (BCI) was achieved in vitro by a chitosan/gelatin sponge (CG) ratio of 5/5 (W/W).
26590895	3	3	theme	spongy	312:317	arg1	material					326:333	This spongy porous material	307:333	This spongy porous material	307:333	This spongy porous material was cross-linked with tannins and then freeze-dried under vacuum to obtain composites containing chitosan and gelatin in different proportions.
26590895	9	4	theme	vascular	1208:1215	arg1	generation					1217:1226	rich vascular generation	1203:1226	rich vascular generation	1203:1226	In addition, subcutaneous transplantation of CG into rabbits resulted in almost complete degradation of CG after 6 weeks, together with rich vascular generation and proliferation in the transplanted region.
26590895	10	5	theme	absorbable	1314:1323	arg1	material					1336:1343	an effective absorbable hemostatic material	1301:1343	an effective absorbable hemostatic material	1301:1343	Thus, CG can be considered an effective absorbable hemostatic material.
26590895	1	6	theme	hemostatic	154:163	arg1	properties					165:174	its hemostatic properties	150:174	its hemostatic properties	150:174	Chitosan is a versatile biological material that is very well known for its hemostatic properties.
26590895	4	7	theme	chitosan/gelatin	562:577	arg1	CG					587:588	CG	587:588	CG	587:588	Results showed that the best blood-clotting index (BCI) was achieved in vitro by a chitosan/gelatin sponge (CG) ratio of 5/5 (W/W).
26590895	4	7	theme	chitosan/gelatin	562:577	arg1	sponge					579:584	chitosan/gelatin sponge	562:584	a chitosan/gelatin sponge (CG) ratio of 5/5 (W/W)	560:608	Results showed that the best blood-clotting index (BCI) was achieved in vitro by a chitosan/gelatin sponge (CG) ratio of 5/5 (W/W).
26590895	7	8	theme	obvious	898:904	arg1	differences					906:916	no obvious differences	895:916	no obvious differences	895:916	However, no obvious differences were observed in thrombin generation with both aPTT and PT tests.
26590895	5	9	theme	liver	688:692	arg1	tests					700:704	liver model tests	688:704	liver model tests	688:704	Furthermore, CG had the best hemostatic effect in rabbit artery bleeding and liver model tests compared to the two components separately.
26590895	6	10	theme	hemostatic	760:769	arg1	due					791:793	due	791:793	due	791:793	The better hemostatic effect of CG may be due to its ability to absorb blood platelets easily and to the higher liquid adsorption ratio.
26590895	6	10	theme	hemostatic	760:769	arg1	effect					771:776	The better hemostatic effect	749:776	The better hemostatic effect of CG	749:782	The better hemostatic effect of CG may be due to its ability to absorb blood platelets easily and to the higher liquid adsorption ratio.
26590895	5	11	theme	model	694:698	arg1	tests					700:704	liver model tests	688:704	liver model tests	688:704	Furthermore, CG had the best hemostatic effect in rabbit artery bleeding and liver model tests compared to the two components separately.
26590895	8	12	theme	Cell	984:987	arg1	tests					998:1002	Cell toxicity tests	984:1002	Cell toxicity tests with L929 cells	984:1018	Cell toxicity tests with L929 cells showed that CG caused no obvious cytotoxicity.
26590895	6	13	theme	blood	820:824	arg1	platelets					826:834	blood platelets	820:834	blood platelets	820:834	The better hemostatic effect of CG may be due to its ability to absorb blood platelets easily and to the higher liquid adsorption ratio.
26590895	2	14	theme	composite	255:263	arg1	properties					230:239	the hemostatic properties	215:239	the hemostatic properties of a chitosan composite obtained from silkworm pupae and gelatin	215:304	The purpose of this study was to test the hemostatic properties of a chitosan composite obtained from silkworm pupae and gelatin.
26590895	0	15	theme	composite	17:25	arg1	sponge					27:32	Chitosan/gelatin composite sponge	0:32	Chitosan/gelatin composite sponge	0:32	Chitosan/gelatin composite sponge is an absorbable surgical hemostatic agent.
26590895	0	15	theme	composite	17:25	arg1	agent					71:75	an absorbable surgical hemostatic agent	37:75	an absorbable surgical hemostatic agent	37:75	Chitosan/gelatin composite sponge is an absorbable surgical hemostatic agent.
26590895	0	16	theme	Chitosan/gelatin	0:15	arg1	sponge					27:32	Chitosan/gelatin composite sponge	0:32	Chitosan/gelatin composite sponge	0:32	Chitosan/gelatin composite sponge is an absorbable surgical hemostatic agent.
26590895	0	16	theme	Chitosan/gelatin	0:15	arg1	agent					71:75	an absorbable surgical hemostatic agent	37:75	an absorbable surgical hemostatic agent	37:75	Chitosan/gelatin composite sponge is an absorbable surgical hemostatic agent.
26590895	9	17	theme	transplanted	1253:1264	arg1	region					1266:1271	the transplanted region	1249:1271	the transplanted region	1249:1271	In addition, subcutaneous transplantation of CG into rabbits resulted in almost complete degradation of CG after 6 weeks, together with rich vascular generation and proliferation in the transplanted region.
26590895	8	18	theme	toxicity	989:996	arg1	tests					998:1002	Cell toxicity tests	984:1002	Cell toxicity tests with L929 cells	984:1018	Cell toxicity tests with L929 cells showed that CG caused no obvious cytotoxicity.
26590895	1	19	theme	versatile	92:100	arg1	Chitosan					78:85	Chitosan	78:85	Chitosan	78:85	Chitosan is a versatile biological material that is very well known for its hemostatic properties.
26590895	1	19	theme	versatile	92:100	arg1	material					113:120	a versatile biological material	90:120	a versatile biological material that is very well known for its hemostatic properties	90:174	Chitosan is a versatile biological material that is very well known for its hemostatic properties.
26590895	8	20	with	tests	998:1002	arg1	cells					1014:1018	L929 cells	1009:1018	L929 cells	1009:1018	Cell toxicity tests with L929 cells showed that CG caused no obvious cytotoxicity.
26590895	8	21	theme	L929	1009:1012	arg1	cells					1014:1018	L929 cells	1009:1018	L929 cells	1009:1018	Cell toxicity tests with L929 cells showed that CG caused no obvious cytotoxicity.
26590895	9	22	theme	complete	1147:1154	arg1	degradation					1156:1166	almost complete degradation	1140:1166	almost complete degradation of CG	1140:1172	In addition, subcutaneous transplantation of CG into rabbits resulted in almost complete degradation of CG after 6 weeks, together with rich vascular generation and proliferation in the transplanted region.
26590895	1	23	theme	biological	102:111	arg1	Chitosan					78:85	Chitosan	78:85	Chitosan	78:85	Chitosan is a versatile biological material that is very well known for its hemostatic properties.
26590895	1	23	theme	biological	102:111	arg1	material					113:120	a versatile biological material	90:120	a versatile biological material that is very well known for its hemostatic properties	90:174	Chitosan is a versatile biological material that is very well known for its hemostatic properties.
26590895	10	24	theme	hemostatic	1325:1334	arg1	material					1336:1343	an effective absorbable hemostatic material	1301:1343	an effective absorbable hemostatic material	1301:1343	Thus, CG can be considered an effective absorbable hemostatic material.
26590895	9	25	from	proliferation	1232:1244	arg1	region					1266:1271	the transplanted region	1249:1271	the transplanted region	1249:1271	In addition, subcutaneous transplantation of CG into rabbits resulted in almost complete degradation of CG after 6 weeks, together with rich vascular generation and proliferation in the transplanted region.
26590895	5	26	theme	rabbit	661:666	arg1	artery					668:673	rabbit artery bleeding	661:682	rabbit artery bleeding	661:682	Furthermore, CG had the best hemostatic effect in rabbit artery bleeding and liver model tests compared to the two components separately.
26590895	0	27	theme	absorbable	40:49	arg1	sponge					27:32	Chitosan/gelatin composite sponge	0:32	Chitosan/gelatin composite sponge	0:32	Chitosan/gelatin composite sponge is an absorbable surgical hemostatic agent.
26590895	0	27	theme	absorbable	40:49	arg1	agent					71:75	an absorbable surgical hemostatic agent	37:75	an absorbable surgical hemostatic agent	37:75	Chitosan/gelatin composite sponge is an absorbable surgical hemostatic agent.
26590895	2	28	theme	hemostatic	219:228	arg1	properties					230:239	the hemostatic properties	215:239	the hemostatic properties of a chitosan composite obtained from silkworm pupae and gelatin	215:304	The purpose of this study was to test the hemostatic properties of a chitosan composite obtained from silkworm pupae and gelatin.
26590895	9	29	from	generation	1217:1226	arg1	region					1266:1271	the transplanted region	1249:1271	the transplanted region	1249:1271	In addition, subcutaneous transplantation of CG into rabbits resulted in almost complete degradation of CG after 6 weeks, together with rich vascular generation and proliferation in the transplanted region.
26590895	5	30	theme	bleeding	675:682	arg1	artery					668:673	rabbit artery bleeding	661:682	rabbit artery bleeding	661:682	Furthermore, CG had the best hemostatic effect in rabbit artery bleeding and liver model tests compared to the two components separately.
26590895	9	31	theme	CG	1171:1172	arg1	degradation					1156:1166	almost complete degradation	1140:1166	almost complete degradation of CG	1140:1172	In addition, subcutaneous transplantation of CG into rabbits resulted in almost complete degradation of CG after 6 weeks, together with rich vascular generation and proliferation in the transplanted region.
26590895	3	32	theme	different	456:464	arg1	proportions					466:476	different proportions	456:476	different proportions	456:476	This spongy porous material was cross-linked with tannins and then freeze-dried under vacuum to obtain composites containing chitosan and gelatin in different proportions.
26590895	0	33	theme	hemostatic	60:69	arg1	sponge					27:32	Chitosan/gelatin composite sponge	0:32	Chitosan/gelatin composite sponge	0:32	Chitosan/gelatin composite sponge is an absorbable surgical hemostatic agent.
26590895	0	33	theme	hemostatic	60:69	arg1	agent					71:75	an absorbable surgical hemostatic agent	37:75	an absorbable surgical hemostatic agent	37:75	Chitosan/gelatin composite sponge is an absorbable surgical hemostatic agent.
26590895	7	34	theme	PT	974:975	arg1	tests					977:981	PT tests	974:981	PT tests	974:981	However, no obvious differences were observed in thrombin generation with both aPTT and PT tests.
26590895	8	35	theme	obvious	1045:1051	arg1	cytotoxicity					1053:1064	no obvious cytotoxicity	1042:1064	no obvious cytotoxicity	1042:1064	Cell toxicity tests with L929 cells showed that CG caused no obvious cytotoxicity.
26590895	9	36	theme	rich	1203:1206	arg1	generation					1217:1226	rich vascular generation	1203:1226	rich vascular generation	1203:1226	In addition, subcutaneous transplantation of CG into rabbits resulted in almost complete degradation of CG after 6 weeks, together with rich vascular generation and proliferation in the transplanted region.
26590895	0	37	theme	surgical	51:58	arg1	sponge					27:32	Chitosan/gelatin composite sponge	0:32	Chitosan/gelatin composite sponge	0:32	Chitosan/gelatin composite sponge is an absorbable surgical hemostatic agent.
26590895	0	37	theme	surgical	51:58	arg1	agent					71:75	an absorbable surgical hemostatic agent	37:75	an absorbable surgical hemostatic agent	37:75	Chitosan/gelatin composite sponge is an absorbable surgical hemostatic agent.
26590895	6	38	theme	adsorption	868:877	arg1	ratio					879:883	the higher liquid adsorption ratio	850:883	the higher liquid adsorption ratio	850:883	The better hemostatic effect of CG may be due to its ability to absorb blood platelets easily and to the higher liquid adsorption ratio.
26590895	6	39	theme	higher	854:859	arg1	ratio					879:883	the higher liquid adsorption ratio	850:883	the higher liquid adsorption ratio	850:883	The better hemostatic effect of CG may be due to its ability to absorb blood platelets easily and to the higher liquid adsorption ratio.
26590895	3	40	contain	containing	421:430	arg2	gelatin					445:451	gelatin	445:451	gelatin	445:451	This spongy porous material was cross-linked with tannins and then freeze-dried under vacuum to obtain composites containing chitosan and gelatin in different proportions.
26590895	3	40	contain	containing	421:430	arg2	chitosan					432:439	chitosan	432:439	chitosan	432:439	This spongy porous material was cross-linked with tannins and then freeze-dried under vacuum to obtain composites containing chitosan and gelatin in different proportions.
26590895	3	40	contain	containing	421:430	arg1	composites					410:419	composites	410:419	composites containing chitosan and gelatin in different proportions	410:476	This spongy porous material was cross-linked with tannins and then freeze-dried under vacuum to obtain composites containing chitosan and gelatin in different proportions.
26590895	7	41	theme	thrombin	935:942	arg1	generation					944:953	thrombin generation	935:953	thrombin generation	935:953	However, no obvious differences were observed in thrombin generation with both aPTT and PT tests.
26590895	6	42	theme	liquid	861:866	arg1	ratio					879:883	the higher liquid adsorption ratio	850:883	the higher liquid adsorption ratio	850:883	The better hemostatic effect of CG may be due to its ability to absorb blood platelets easily and to the higher liquid adsorption ratio.
26590895	5	43	theme	hemostatic	640:649	arg1	effect					651:656	the best hemostatic effect	631:656	the best hemostatic effect in rabbit artery bleeding and liver model tests	631:704	Furthermore, CG had the best hemostatic effect in rabbit artery bleeding and liver model tests compared to the two components separately.
26590895	2	44	theme	study	197:201	arg1	purpose					181:187	The purpose	177:187	The purpose of this study	177:201	The purpose of this study was to test the hemostatic properties of a chitosan composite obtained from silkworm pupae and gelatin.
26590895	2	45	theme	chitosan	246:253	arg1	composite					255:263	a chitosan composite	244:263	a chitosan composite obtained from silkworm pupae and gelatin	244:304	The purpose of this study was to test the hemostatic properties of a chitosan composite obtained from silkworm pupae and gelatin.
26590895	9	46	theme	CG	1112:1113	arg1	transplantation					1093:1107	subcutaneous transplantation	1080:1107	subcutaneous transplantation of CG into rabbits	1080:1126	In addition, subcutaneous transplantation of CG into rabbits resulted in almost complete degradation of CG after 6 weeks, together with rich vascular generation and proliferation in the transplanted region.
26590895	5	47	contain	had	627:629	arg2	effect					651:656	the best hemostatic effect	631:656	the best hemostatic effect in rabbit artery bleeding and liver model tests	631:704	Furthermore, CG had the best hemostatic effect in rabbit artery bleeding and liver model tests compared to the two components separately.
26590895	5	47	contain	had	627:629	arg1	CG					624:625	CG	624:625	CG	624:625	Furthermore, CG had the best hemostatic effect in rabbit artery bleeding and liver model tests compared to the two components separately.
26590895	10	48	theme	effective	1304:1312	arg1	material					1336:1343	an effective absorbable hemostatic material	1301:1343	an effective absorbable hemostatic material	1301:1343	Thus, CG can be considered an effective absorbable hemostatic material.
26590895	5	49	from	effect	651:656	arg1	tests					700:704	liver model tests	688:704	liver model tests	688:704	Furthermore, CG had the best hemostatic effect in rabbit artery bleeding and liver model tests compared to the two components separately.
26590895	5	49	from	effect	651:656	arg1	artery					668:673	rabbit artery bleeding	661:682	rabbit artery bleeding	661:682	Furthermore, CG had the best hemostatic effect in rabbit artery bleeding and liver model tests compared to the two components separately.
26590895	3	50	theme	porous	319:324	arg1	material					326:333	This spongy porous material	307:333	This spongy porous material	307:333	This spongy porous material was cross-linked with tannins and then freeze-dried under vacuum to obtain composites containing chitosan and gelatin in different proportions.
26590895	6	51	theme	CG	781:782	arg1	due					791:793	due	791:793	due	791:793	The better hemostatic effect of CG may be due to its ability to absorb blood platelets easily and to the higher liquid adsorption ratio.
26590895	6	51	theme	CG	781:782	arg1	effect					771:776	The better hemostatic effect	749:776	The better hemostatic effect of CG	749:782	The better hemostatic effect of CG may be due to its ability to absorb blood platelets easily and to the higher liquid adsorption ratio.
26590895	4	52	theme	5/5	600:602	arg1	ratio					591:595	a chitosan/gelatin sponge (CG) ratio	560:595	a chitosan/gelatin sponge (CG) ratio of 5/5 (W/W)	560:608	Results showed that the best blood-clotting index (BCI) was achieved in vitro by a chitosan/gelatin sponge (CG) ratio of 5/5 (W/W).
26590895	7	53	located	observed	923:930	arg1	generation					944:953	thrombin generation	935:953	thrombin generation	935:953	However, no obvious differences were observed in thrombin generation with both aPTT and PT tests.
26590895	7	53	located	observed	923:930	arg2	differences					906:916	no obvious differences	895:916	no obvious differences	895:916	However, no obvious differences were observed in thrombin generation with both aPTT and PT tests.
26590895	4	54	theme	blood-clotting	508:521	arg1	BCI					530:532	BCI	530:532	BCI	530:532	Results showed that the best blood-clotting index (BCI) was achieved in vitro by a chitosan/gelatin sponge (CG) ratio of 5/5 (W/W).
26590895	4	54	theme	blood-clotting	508:521	arg1	index					523:527	the best blood-clotting index	499:527	the best blood-clotting index (BCI)	499:533	Results showed that the best blood-clotting index (BCI) was achieved in vitro by a chitosan/gelatin sponge (CG) ratio of 5/5 (W/W).
26590895	9	55	theme	subcutaneous	1080:1091	arg1	transplantation					1093:1107	subcutaneous transplantation	1080:1107	subcutaneous transplantation of CG into rabbits	1080:1126	In addition, subcutaneous transplantation of CG into rabbits resulted in almost complete degradation of CG after 6 weeks, together with rich vascular generation and proliferation in the transplanted region.
28398127	7	0	theme	culture	1156:1162	arg1	weeks					1147:1151	4 weeks	1145:1151	4 weeks	1145:1151	The NIR-determined matrix absorbance plateaued after 4 weeks of culture, which was in agreement with the biochemical assay results.
28398127	11	1	theme	destructive	1828:1838	arg1	methods					1867:1873	destructive biochemical and mechanical methods	1828:1873	destructive biochemical and mechanical methods	1828:1873	Collectively, these data suggest that NIR spectral data analysis could be an alternative to destructive biochemical and mechanical methods for evaluation of HA-based engineered cartilage construct properties.
28398127	2	2	theme	time	244:247	arg1	point					249:253	the optimal time point	232:253	the optimal time point to harvest HA-based engineered constructs for cartilage repair	232:316	However, the optimal time point to harvest HA-based engineered constructs for cartilage repair is still under investigation.
28398127	7	3	theme	biochemical	1197:1207	arg1	results					1215:1221	the biochemical assay results	1193:1221	the biochemical assay results	1193:1221	The NIR-determined matrix absorbance plateaued after 4 weeks of culture, which was in agreement with the biochemical assay results.
28398127	1	4	theme	tissue	190:195	arg1	applications					209:220	cartilage tissue engineering applications	180:220	cartilage tissue engineering applications	180:220	Hyaluronic acid (HA) has been widely used for cartilage tissue engineering applications.
28398127	7	5	with	agreement	1178:1186	arg1	results					1215:1221	the biochemical assay results	1193:1221	the biochemical assay results	1193:1221	The NIR-determined matrix absorbance plateaued after 4 weeks of culture, which was in agreement with the biochemical assay results.
28398127	2	6	theme	optimal	236:242	arg1	point					249:253	the optimal time point	232:253	the optimal time point to harvest HA-based engineered constructs for cartilage repair	232:316	However, the optimal time point to harvest HA-based engineered constructs for cartilage repair is still under investigation.
28398127	10	7	theme	maximum	1567:1573	arg1	increase					1575:1582	the maximum increase	1563:1582	the maximum increase in moduli	1563:1592	Furthermore, the maximum increase in moduli was calculated from the first derivative of the curve fit of NIR-predicted and actual moduli values over time, and both occurred at ∼2 weeks.
28398127	7	8	theme	NIR-determined	1096:1109	arg1	absorbance					1118:1127	The NIR-determined matrix absorbance	1092:1127	The NIR-determined matrix absorbance	1092:1127	The NIR-determined matrix absorbance plateaued after 4 weeks of culture, which was in agreement with the biochemical assay results.
28398127	3	9	theme	cartilage	539:547	arg1	constructs					549:558	HA-based engineered cartilage constructs	519:558	HA-based engineered cartilage constructs	519:558	In this study, we investigated the ability of a nondestructive modality, near-infrared spectroscopic (NIR) analysis, to predict compositional and mechanical properties of HA-based engineered cartilage constructs.
28398127	6	10	theme	NIR	980:982	arg1	absorbances					984:994	NIR absorbances	980:994	NIR absorbances associated with the scaffold material, water, and engineered cartilage matrix,	980:1073	NIR absorbances associated with the scaffold material, water, and engineered cartilage matrix, were identified.
28398127	11	11	theme	data	1787:1790	arg1	alternative					1813:1823	an alternative	1810:1823	an alternative to destructive biochemical and mechanical methods for evaluation of HA-based engineered cartilage construct properties	1810:1942	Collectively, these data suggest that NIR spectral data analysis could be an alternative to destructive biochemical and mechanical methods for evaluation of HA-based engineered cartilage construct properties.
28398127	11	11	theme	data	1787:1790	arg1	analysis					1792:1799	NIR spectral data analysis	1774:1799	NIR spectral data analysis	1774:1799	Collectively, these data suggest that NIR spectral data analysis could be an alternative to destructive biochemical and mechanical methods for evaluation of HA-based engineered cartilage construct properties.
28398127	11	12	theme	NIR	1774:1776	arg1	alternative					1813:1823	an alternative	1810:1823	an alternative to destructive biochemical and mechanical methods for evaluation of HA-based engineered cartilage construct properties	1810:1942	Collectively, these data suggest that NIR spectral data analysis could be an alternative to destructive biochemical and mechanical methods for evaluation of HA-based engineered cartilage construct properties.
28398127	11	12	theme	NIR	1774:1776	arg1	analysis					1792:1799	NIR spectral data analysis	1774:1799	NIR spectral data analysis	1774:1799	Collectively, these data suggest that NIR spectral data analysis could be an alternative to destructive biochemical and mechanical methods for evaluation of HA-based engineered cartilage construct properties.
28398127	11	13	theme	properties	1933:1942	arg1	evaluation					1879:1888	evaluation	1879:1888	evaluation of HA-based engineered cartilage construct properties	1879:1942	Collectively, these data suggest that NIR spectral data analysis could be an alternative to destructive biochemical and mechanical methods for evaluation of HA-based engineered cartilage construct properties.
28398127	9	14	theme	value	1481:1485	arg1	error					1462:1466	a prediction error	1449:1466	a prediction error of 10% and R value of 0.88 for predicted versus actual values of dynamic modulus	1449:1547	A multivariate partial least square model based on NIR spectral input was developed to predict the moduli of the constructs, which resulted in a prediction error of 10% and R value of 0.88 for predicted versus actual values of dynamic modulus.
28398127	2	15	theme	engineered	275:284	arg1	constructs					286:295	harvest HA-based engineered constructs	258:295	harvest HA-based engineered constructs	258:295	However, the optimal time point to harvest HA-based engineered constructs for cartilage repair is still under investigation.
28398127	9	16	theme	0.88	1490:1493	arg1	0.88					1490:1493	0.88	1490:1493	0.88	1490:1493	A multivariate partial least square model based on NIR spectral input was developed to predict the moduli of the constructs, which resulted in a prediction error of 10% and R value of 0.88 for predicted versus actual values of dynamic modulus.
28398127	9	16	theme	0.88	1490:1493	arg1	%					1473:1473	10%	1471:1473	10%	1471:1473	A multivariate partial least square model based on NIR spectral input was developed to predict the moduli of the constructs, which resulted in a prediction error of 10% and R value of 0.88 for predicted versus actual values of dynamic modulus.
28398127	9	16	theme	0.88	1490:1493	arg1	value					1481:1485	R value	1479:1485	R value of 0.88 for predicted versus actual values of dynamic modulus	1479:1547	A multivariate partial least square model based on NIR spectral input was developed to predict the moduli of the constructs, which resulted in a prediction error of 10% and R value of 0.88 for predicted versus actual values of dynamic modulus.
28398127	10	17	theme	NIR-predicted	1655:1667	arg1	values					1687:1692	NIR-predicted and actual moduli values	1655:1692	NIR-predicted and actual moduli values	1655:1692	Furthermore, the maximum increase in moduli was calculated from the first derivative of the curve fit of NIR-predicted and actual moduli values over time, and both occurred at ∼2 weeks.
28398127	0	18	theme	Hyaluronic	79:88	arg1	Constructs					122:131	Hyaluronic Acid-Based Engineered Cartilage Constructs	79:131	Hyaluronic Acid-Based Engineered Cartilage Constructs	79:131	Near-Infrared Spectroscopy Predicts Compositional and Mechanical Properties of Hyaluronic Acid-Based Engineered Cartilage Constructs.
28398127	2	19	theme	harvest	258:264	arg1	constructs					286:295	harvest HA-based engineered constructs	258:295	harvest HA-based engineered constructs	258:295	However, the optimal time point to harvest HA-based engineered constructs for cartilage repair is still under investigation.
28398127	9	20	theme	predicted	1499:1507	arg1	values					1523:1528	predicted versus actual values	1499:1528	predicted versus actual values of dynamic modulus	1499:1547	A multivariate partial least square model based on NIR spectral input was developed to predict the moduli of the constructs, which resulted in a prediction error of 10% and R value of 0.88 for predicted versus actual values of dynamic modulus.
28398127	0	21	theme	Engineered	101:110	arg1	Constructs					122:131	Hyaluronic Acid-Based Engineered Cartilage Constructs	79:131	Hyaluronic Acid-Based Engineered Cartilage Constructs	79:131	Near-Infrared Spectroscopy Predicts Compositional and Mechanical Properties of Hyaluronic Acid-Based Engineered Cartilage Constructs.
28398127	9	22	theme	multivariate	1308:1319	arg1	model					1342:1346	A multivariate partial least square model	1306:1346	A multivariate partial least square model based on NIR spectral input	1306:1374	A multivariate partial least square model based on NIR spectral input was developed to predict the moduli of the constructs, which resulted in a prediction error of 10% and R value of 0.88 for predicted versus actual values of dynamic modulus.
28398127	9	23	theme	actual	1516:1521	arg1	values					1523:1528	predicted versus actual values	1499:1528	predicted versus actual values of dynamic modulus	1499:1547	A multivariate partial least square model based on NIR spectral input was developed to predict the moduli of the constructs, which resulted in a prediction error of 10% and R value of 0.88 for predicted versus actual values of dynamic modulus.
28398127	0	24	theme	Constructs	122:131	arg1	Compositional					36:48	Compositional	36:48	Compositional	36:48	Near-Infrared Spectroscopy Predicts Compositional and Mechanical Properties of Hyaluronic Acid-Based Engineered Cartilage Constructs.
28398127	0	24	theme	Constructs	122:131	arg1	Properties					65:74	Mechanical Properties	54:74	Mechanical Properties	54:74	Near-Infrared Spectroscopy Predicts Compositional and Mechanical Properties of Hyaluronic Acid-Based Engineered Cartilage Constructs.
28398127	9	25	theme	modulus	1541:1547	arg1	values					1523:1528	predicted versus actual values	1499:1528	predicted versus actual values of dynamic modulus	1499:1547	A multivariate partial least square model based on NIR spectral input was developed to predict the moduli of the constructs, which resulted in a prediction error of 10% and R value of 0.88 for predicted versus actual values of dynamic modulus.
28398127	3	26	theme	nondestructive	396:409	arg1	analysis					455:462	near-infrared spectroscopic (NIR) analysis	421:462	near-infrared spectroscopic (NIR) analysis	421:462	In this study, we investigated the ability of a nondestructive modality, near-infrared spectroscopic (NIR) analysis, to predict compositional and mechanical properties of HA-based engineered cartilage constructs.
28398127	3	26	theme	nondestructive	396:409	arg1	modality					411:418	a nondestructive modality	394:418	a nondestructive modality	394:418	In this study, we investigated the ability of a nondestructive modality, near-infrared spectroscopic (NIR) analysis, to predict compositional and mechanical properties of HA-based engineered cartilage constructs.
28398127	11	27	theme	mechanical	1856:1865	arg1	methods					1867:1873	destructive biochemical and mechanical methods	1828:1873	destructive biochemical and mechanical methods	1828:1873	Collectively, these data suggest that NIR spectral data analysis could be an alternative to destructive biochemical and mechanical methods for evaluation of HA-based engineered cartilage construct properties.
28398127	0	28	theme	Near-Infrared	0:12	arg1	Spectroscopy					14:25	Near-Infrared Spectroscopy	0:25	Near-Infrared Spectroscopy	0:25	Near-Infrared Spectroscopy Predicts Compositional and Mechanical Properties of Hyaluronic Acid-Based Engineered Cartilage Constructs.
28398127	5	29	theme	compression	961:971	arg1	tests					973:977	unconfined compression tests	950:977	unconfined compression tests	950:977	Constructs were harvested at 2, 4, 6, and 8 weeks, collagen and sulfated glycosaminoglycan content measured using biochemical assays, and the mechanical properties of the constructs evaluated using unconfined compression tests.
28398127	9	30	dep	partial	1321:1327	arg1	square					1335:1340	square	1335:1340	square	1335:1340	A multivariate partial least square model based on NIR spectral input was developed to predict the moduli of the constructs, which resulted in a prediction error of 10% and R value of 0.88 for predicted versus actual values of dynamic modulus.
28398127	11	31	theme	engineered	1902:1911	arg1	properties					1933:1942	HA-based engineered cartilage construct properties	1893:1942	HA-based engineered cartilage construct properties	1893:1942	Collectively, these data suggest that NIR spectral data analysis could be an alternative to destructive biochemical and mechanical methods for evaluation of HA-based engineered cartilage construct properties.
28398127	5	32	theme	sulfated	816:823	arg1	content					843:849	sulfated glycosaminoglycan content	816:849	sulfated glycosaminoglycan content	816:849	Constructs were harvested at 2, 4, 6, and 8 weeks, collagen and sulfated glycosaminoglycan content measured using biochemical assays, and the mechanical properties of the constructs evaluated using unconfined compression tests.
28398127	9	33	theme	spectral	1361:1368	arg1	input					1370:1374	NIR spectral input	1357:1374	NIR spectral input	1357:1374	A multivariate partial least square model based on NIR spectral input was developed to predict the moduli of the constructs, which resulted in a prediction error of 10% and R value of 0.88 for predicted versus actual values of dynamic modulus.
28398127	3	34	theme	spectroscopic	435:447	arg1	analysis					455:462	near-infrared spectroscopic (NIR) analysis	421:462	near-infrared spectroscopic (NIR) analysis	421:462	In this study, we investigated the ability of a nondestructive modality, near-infrared spectroscopic (NIR) analysis, to predict compositional and mechanical properties of HA-based engineered cartilage constructs.
28398127	3	34	theme	spectroscopic	435:447	arg1	modality					411:418	a nondestructive modality	394:418	a nondestructive modality	394:418	In this study, we investigated the ability of a nondestructive modality, near-infrared spectroscopic (NIR) analysis, to predict compositional and mechanical properties of HA-based engineered cartilage constructs.
28398127	4	35	theme	control	599:605	arg1	constructs					617:626	control, unseeded constructs	599:626	control, unseeded constructs	599:626	NIR spectral data were collected from control, unseeded constructs, and twice per week by fiber optic from constructs seeded with chondrocytes during their development over an 8-week period.
28398127	10	36	theme	fit	1648:1650	arg1	derivative					1624:1633	the first derivative	1614:1633	the first derivative of the curve fit of NIR-predicted and actual moduli values over time	1614:1702	Furthermore, the maximum increase in moduli was calculated from the first derivative of the curve fit of NIR-predicted and actual moduli values over time, and both occurred at ∼2 weeks.
28398127	5	37	theme	biochemical	866:876	arg1	assays					878:883	biochemical assays	866:883	biochemical assays	866:883	Constructs were harvested at 2, 4, 6, and 8 weeks, collagen and sulfated glycosaminoglycan content measured using biochemical assays, and the mechanical properties of the constructs evaluated using unconfined compression tests.
28398127	6	38	theme	cartilage	1057:1065	arg1	matrix					1067:1072	engineered cartilage matrix	1046:1072	engineered cartilage matrix	1046:1072	NIR absorbances associated with the scaffold material, water, and engineered cartilage matrix, were identified.
28398127	4	39	theme	NIR	561:563	arg1	data					574:577	NIR spectral data	561:577	NIR spectral data	561:577	NIR spectral data were collected from control, unseeded constructs, and twice per week by fiber optic from constructs seeded with chondrocytes during their development over an 8-week period.
28398127	10	40	theme	values	1687:1692	arg1	fit					1648:1650	the curve fit	1638:1650	the curve fit of NIR-predicted and actual moduli values over time	1638:1702	Furthermore, the maximum increase in moduli was calculated from the first derivative of the curve fit of NIR-predicted and actual moduli values over time, and both occurred at ∼2 weeks.
28398127	3	41	theme	mechanical	494:503	arg1	properties					505:514	compositional and mechanical properties	476:514	compositional and mechanical properties of HA-based engineered cartilage constructs	476:558	In this study, we investigated the ability of a nondestructive modality, near-infrared spectroscopic (NIR) analysis, to predict compositional and mechanical properties of HA-based engineered cartilage constructs.
28398127	5	42	theme	mechanical	894:903	arg1	properties					905:914	the mechanical properties	890:914	the mechanical properties of the constructs	890:932	Constructs were harvested at 2, 4, 6, and 8 weeks, collagen and sulfated glycosaminoglycan content measured using biochemical assays, and the mechanical properties of the constructs evaluated using unconfined compression tests.
28398127	10	43	theme	curve	1642:1646	arg1	fit					1648:1650	the curve fit	1638:1650	the curve fit of NIR-predicted and actual moduli values over time	1638:1702	Furthermore, the maximum increase in moduli was calculated from the first derivative of the curve fit of NIR-predicted and actual moduli values over time, and both occurred at ∼2 weeks.
28398127	3	44	theme	constructs	549:558	arg1	properties					505:514	compositional and mechanical properties	476:514	compositional and mechanical properties of HA-based engineered cartilage constructs	476:558	In this study, we investigated the ability of a nondestructive modality, near-infrared spectroscopic (NIR) analysis, to predict compositional and mechanical properties of HA-based engineered cartilage constructs.
28398127	10	45	from	increase	1575:1582	arg1	moduli					1587:1592	moduli	1587:1592	moduli	1587:1592	Furthermore, the maximum increase in moduli was calculated from the first derivative of the curve fit of NIR-predicted and actual moduli values over time, and both occurred at ∼2 weeks.
28398127	3	46	theme	engineered	528:537	arg1	constructs					549:558	HA-based engineered cartilage constructs	519:558	HA-based engineered cartilage constructs	519:558	In this study, we investigated the ability of a nondestructive modality, near-infrared spectroscopic (NIR) analysis, to predict compositional and mechanical properties of HA-based engineered cartilage constructs.
28398127	9	47	theme	constructs	1419:1428	arg1	moduli					1405:1410	the moduli	1401:1410	the moduli of the constructs, which resulted in a prediction error of 10% and R value of 0.88 for predicted versus actual values of dynamic modulus	1401:1547	A multivariate partial least square model based on NIR spectral input was developed to predict the moduli of the constructs, which resulted in a prediction error of 10% and R value of 0.88 for predicted versus actual values of dynamic modulus.
28398127	5	48	theme	constructs	923:932	arg1	properties					905:914	the mechanical properties	890:914	the mechanical properties of the constructs	890:932	Constructs were harvested at 2, 4, 6, and 8 weeks, collagen and sulfated glycosaminoglycan content measured using biochemical assays, and the mechanical properties of the constructs evaluated using unconfined compression tests.
28398127	1	49	used	used	171:174	arg2	acid					145:148	Hyaluronic acid	134:148	Hyaluronic acid (HA)	134:153	Hyaluronic acid (HA) has been widely used for cartilage tissue engineering applications.
28398127	1	49	used	used	171:174	arg2	HA					151:152	HA	151:152	HA	151:152	Hyaluronic acid (HA) has been widely used for cartilage tissue engineering applications.
28398127	2	50	theme	cartilage	301:309	arg1	repair					311:316	cartilage repair	301:316	cartilage repair	301:316	However, the optimal time point to harvest HA-based engineered constructs for cartilage repair is still under investigation.
28398127	1	51	theme	engineering	197:207	arg1	applications					209:220	cartilage tissue engineering applications	180:220	cartilage tissue engineering applications	180:220	Hyaluronic acid (HA) has been widely used for cartilage tissue engineering applications.
28398127	1	52	theme	cartilage	180:188	arg1	applications					209:220	cartilage tissue engineering applications	180:220	cartilage tissue engineering applications	180:220	Hyaluronic acid (HA) has been widely used for cartilage tissue engineering applications.
28398127	7	53	theme	matrix	1111:1116	arg1	absorbance					1118:1127	The NIR-determined matrix absorbance	1092:1127	The NIR-determined matrix absorbance	1092:1127	The NIR-determined matrix absorbance plateaued after 4 weeks of culture, which was in agreement with the biochemical assay results.
28398127	9	54	theme	prediction	1451:1460	arg1	error					1462:1466	a prediction error	1449:1466	a prediction error of 10% and R value of 0.88 for predicted versus actual values of dynamic modulus	1449:1547	A multivariate partial least square model based on NIR spectral input was developed to predict the moduli of the constructs, which resulted in a prediction error of 10% and R value of 0.88 for predicted versus actual values of dynamic modulus.
28398127	8	55	theme	constructs	1268:1277	arg1	properties					1250:1259	the mechanical properties	1235:1259	the mechanical properties of the constructs	1235:1277	Similarly, the mechanical properties of the constructs also plateaued at 4 weeks.
28398127	5	56	theme	glycosaminoglycan	825:841	arg1	content					843:849	sulfated glycosaminoglycan content	816:849	sulfated glycosaminoglycan content	816:849	Constructs were harvested at 2, 4, 6, and 8 weeks, collagen and sulfated glycosaminoglycan content measured using biochemical assays, and the mechanical properties of the constructs evaluated using unconfined compression tests.
28398127	3	57	theme	compositional	476:488	arg1	properties					505:514	compositional and mechanical properties	476:514	compositional and mechanical properties of HA-based engineered cartilage constructs	476:558	In this study, we investigated the ability of a nondestructive modality, near-infrared spectroscopic (NIR) analysis, to predict compositional and mechanical properties of HA-based engineered cartilage constructs.
28398127	4	58	theme	fiber	651:655	arg1	optic					657:661	fiber optic	651:661	fiber optic	651:661	NIR spectral data were collected from control, unseeded constructs, and twice per week by fiber optic from constructs seeded with chondrocytes during their development over an 8-week period.
28398127	3	59	theme	HA-based	519:526	arg1	constructs					549:558	HA-based engineered cartilage constructs	519:558	HA-based engineered cartilage constructs	519:558	In this study, we investigated the ability of a nondestructive modality, near-infrared spectroscopic (NIR) analysis, to predict compositional and mechanical properties of HA-based engineered cartilage constructs.
28398127	9	60	theme	%	1473:1473	arg1	error					1462:1466	a prediction error	1449:1466	a prediction error of 10% and R value of 0.88 for predicted versus actual values of dynamic modulus	1449:1547	A multivariate partial least square model based on NIR spectral input was developed to predict the moduli of the constructs, which resulted in a prediction error of 10% and R value of 0.88 for predicted versus actual values of dynamic modulus.
28398127	11	61	theme	construct	1923:1931	arg1	properties					1933:1942	HA-based engineered cartilage construct properties	1893:1942	HA-based engineered cartilage construct properties	1893:1942	Collectively, these data suggest that NIR spectral data analysis could be an alternative to destructive biochemical and mechanical methods for evaluation of HA-based engineered cartilage construct properties.
28398127	9	62	theme	R	1479:1479	arg1	value					1481:1485	R value	1479:1485	R value of 0.88 for predicted versus actual values of dynamic modulus	1479:1547	A multivariate partial least square model based on NIR spectral input was developed to predict the moduli of the constructs, which resulted in a prediction error of 10% and R value of 0.88 for predicted versus actual values of dynamic modulus.
28398127	8	63	theme	mechanical	1239:1248	arg1	properties					1250:1259	the mechanical properties	1235:1259	the mechanical properties of the constructs	1235:1277	Similarly, the mechanical properties of the constructs also plateaued at 4 weeks.
28398127	4	64	theme	8-week	737:742	arg1	period					744:749	an 8-week period	734:749	an 8-week period	734:749	NIR spectral data were collected from control, unseeded constructs, and twice per week by fiber optic from constructs seeded with chondrocytes during their development over an 8-week period.
28398127	11	65	theme	spectral	1778:1785	arg1	alternative					1813:1823	an alternative	1810:1823	an alternative to destructive biochemical and mechanical methods for evaluation of HA-based engineered cartilage construct properties	1810:1942	Collectively, these data suggest that NIR spectral data analysis could be an alternative to destructive biochemical and mechanical methods for evaluation of HA-based engineered cartilage construct properties.
28398127	11	65	theme	spectral	1778:1785	arg1	analysis					1792:1799	NIR spectral data analysis	1774:1799	NIR spectral data analysis	1774:1799	Collectively, these data suggest that NIR spectral data analysis could be an alternative to destructive biochemical and mechanical methods for evaluation of HA-based engineered cartilage construct properties.
28398127	6	66	theme	engineered	1046:1055	arg1	matrix					1067:1072	engineered cartilage matrix	1046:1072	engineered cartilage matrix	1046:1072	NIR absorbances associated with the scaffold material, water, and engineered cartilage matrix, were identified.
28398127	10	67	theme	actual	1673:1678	arg1	values					1687:1692	NIR-predicted and actual moduli values	1655:1692	NIR-predicted and actual moduli values	1655:1692	Furthermore, the maximum increase in moduli was calculated from the first derivative of the curve fit of NIR-predicted and actual moduli values over time, and both occurred at ∼2 weeks.
28398127	0	68	theme	Acid-Based	90:99	arg1	Constructs					122:131	Hyaluronic Acid-Based Engineered Cartilage Constructs	79:131	Hyaluronic Acid-Based Engineered Cartilage Constructs	79:131	Near-Infrared Spectroscopy Predicts Compositional and Mechanical Properties of Hyaluronic Acid-Based Engineered Cartilage Constructs.
28398127	2	69	theme	HA-based	266:273	arg1	constructs					286:295	harvest HA-based engineered constructs	258:295	harvest HA-based engineered constructs	258:295	However, the optimal time point to harvest HA-based engineered constructs for cartilage repair is still under investigation.
28398127	0	70	theme	Cartilage	112:120	arg1	Constructs					122:131	Hyaluronic Acid-Based Engineered Cartilage Constructs	79:131	Hyaluronic Acid-Based Engineered Cartilage Constructs	79:131	Near-Infrared Spectroscopy Predicts Compositional and Mechanical Properties of Hyaluronic Acid-Based Engineered Cartilage Constructs.
28398127	9	71	theme	partial	1321:1327	arg1	model					1342:1346	A multivariate partial least square model	1306:1346	A multivariate partial least square model based on NIR spectral input	1306:1374	A multivariate partial least square model based on NIR spectral input was developed to predict the moduli of the constructs, which resulted in a prediction error of 10% and R value of 0.88 for predicted versus actual values of dynamic modulus.
28398127	6	72	theme	scaffold	1016:1023	arg1	material					1025:1032	the scaffold material	1012:1032	the scaffold material	1012:1032	NIR absorbances associated with the scaffold material, water, and engineered cartilage matrix, were identified.
28398127	7	73	theme	assay	1209:1213	arg1	results					1215:1221	the biochemical assay results	1193:1221	the biochemical assay results	1193:1221	The NIR-determined matrix absorbance plateaued after 4 weeks of culture, which was in agreement with the biochemical assay results.
28398127	4	74	dep	control	599:605	arg1	unseeded					608:615	unseeded	608:615	unseeded	608:615	NIR spectral data were collected from control, unseeded constructs, and twice per week by fiber optic from constructs seeded with chondrocytes during their development over an 8-week period.
28398127	9	75	theme	dynamic	1533:1539	arg1	modulus					1541:1547	dynamic modulus	1533:1547	dynamic modulus	1533:1547	A multivariate partial least square model based on NIR spectral input was developed to predict the moduli of the constructs, which resulted in a prediction error of 10% and R value of 0.88 for predicted versus actual values of dynamic modulus.
28398127	5	76	theme	unconfined	950:959	arg1	tests					973:977	unconfined compression tests	950:977	unconfined compression tests	950:977	Constructs were harvested at 2, 4, 6, and 8 weeks, collagen and sulfated glycosaminoglycan content measured using biochemical assays, and the mechanical properties of the constructs evaluated using unconfined compression tests.
28398127	3	77	theme	modality	411:418	arg1	ability					383:389	the ability	379:389	the ability of a nondestructive modality, near-infrared spectroscopic (NIR) analysis, to predict compositional and mechanical properties of HA-based engineered cartilage constructs	379:558	In this study, we investigated the ability of a nondestructive modality, near-infrared spectroscopic (NIR) analysis, to predict compositional and mechanical properties of HA-based engineered cartilage constructs.
28398127	10	78	theme	first	1618:1622	arg1	derivative					1624:1633	the first derivative	1614:1633	the first derivative of the curve fit of NIR-predicted and actual moduli values over time	1614:1702	Furthermore, the maximum increase in moduli was calculated from the first derivative of the curve fit of NIR-predicted and actual moduli values over time, and both occurred at ∼2 weeks.
28398127	9	79	theme	NIR	1357:1359	arg1	input					1370:1374	NIR spectral input	1357:1374	NIR spectral input	1357:1374	A multivariate partial least square model based on NIR spectral input was developed to predict the moduli of the constructs, which resulted in a prediction error of 10% and R value of 0.88 for predicted versus actual values of dynamic modulus.
28398127	1	80	theme	Hyaluronic	134:143	arg1	HA					151:152	HA	151:152	HA	151:152	Hyaluronic acid (HA) has been widely used for cartilage tissue engineering applications.
28398127	1	80	theme	Hyaluronic	134:143	arg1	acid					145:148	Hyaluronic acid	134:148	Hyaluronic acid (HA)	134:153	Hyaluronic acid (HA) has been widely used for cartilage tissue engineering applications.
28398127	11	81	theme	cartilage	1913:1921	arg1	properties					1933:1942	HA-based engineered cartilage construct properties	1893:1942	HA-based engineered cartilage construct properties	1893:1942	Collectively, these data suggest that NIR spectral data analysis could be an alternative to destructive biochemical and mechanical methods for evaluation of HA-based engineered cartilage construct properties.
28398127	0	82	theme	Mechanical	54:63	arg1	Properties					65:74	Mechanical Properties	54:74	Mechanical Properties	54:74	Near-Infrared Spectroscopy Predicts Compositional and Mechanical Properties of Hyaluronic Acid-Based Engineered Cartilage Constructs.
28398127	11	83	theme	biochemical	1840:1850	arg1	methods					1867:1873	destructive biochemical and mechanical methods	1828:1873	destructive biochemical and mechanical methods	1828:1873	Collectively, these data suggest that NIR spectral data analysis could be an alternative to destructive biochemical and mechanical methods for evaluation of HA-based engineered cartilage construct properties.
28398127	4	84	theme	spectral	565:572	arg1	data					574:577	NIR spectral data	561:577	NIR spectral data	561:577	NIR spectral data were collected from control, unseeded constructs, and twice per week by fiber optic from constructs seeded with chondrocytes during their development over an 8-week period.
28398127	11	85	theme	HA-based	1893:1900	arg1	properties					1933:1942	HA-based engineered cartilage construct properties	1893:1942	HA-based engineered cartilage construct properties	1893:1942	Collectively, these data suggest that NIR spectral data analysis could be an alternative to destructive biochemical and mechanical methods for evaluation of HA-based engineered cartilage construct properties.
28398127	3	86	theme	near-infrared	421:433	arg1	NIR					450:452	NIR	450:452	NIR	450:452	In this study, we investigated the ability of a nondestructive modality, near-infrared spectroscopic (NIR) analysis, to predict compositional and mechanical properties of HA-based engineered cartilage constructs.
28398127	3	86	theme	near-infrared	421:433	arg1	spectroscopic					435:447	near-infrared spectroscopic	421:447	near-infrared spectroscopic (NIR) analysis	421:462	In this study, we investigated the ability of a nondestructive modality, near-infrared spectroscopic (NIR) analysis, to predict compositional and mechanical properties of HA-based engineered cartilage constructs.
28398127	10	87	theme	moduli	1680:1685	arg1	values					1687:1692	NIR-predicted and actual moduli values	1655:1692	NIR-predicted and actual moduli values	1655:1692	Furthermore, the maximum increase in moduli was calculated from the first derivative of the curve fit of NIR-predicted and actual moduli values over time, and both occurred at ∼2 weeks.
24997793	11	0	theme	inhibitory	2116:2125	arg1	effects					2127:2133	the inhibitory effects	2112:2133	the inhibitory effects of lignin	2112:2143	These data indicate that whereas PcGCE expression in hybrid aspen increases lignin deposition, the inhibitory effects of lignin are efficiently removed during acid pretreatment, and the extent of wood cellulose conversion during hydrolysis after acid pretreatment is improved in the transgenic lines possible due to reduced cell wall cross-links between cell wall biopolymers by PcGCE.
24997793	6	1	theme	lignin	1110:1115	arg1	content					1117:1123	lignin content	1110:1123	lignin content	1110:1123	Diffuse reflectance FT-IR spectroscopy and pyrolysis-GC/MS analyses showed significant alternation in wood chemistry of transgenic plants including an increase in lignin content and S/G ratio, and a decrease in carbohydrate content.
24997793	11	2	theme	cell	2371:2374	arg1	biopolymers					2381:2391	cell wall biopolymers	2371:2391	cell wall biopolymers by PcGCE	2371:2400	These data indicate that whereas PcGCE expression in hybrid aspen increases lignin deposition, the inhibitory effects of lignin are efficiently removed during acid pretreatment, and the extent of wood cellulose conversion during hydrolysis after acid pretreatment is improved in the transgenic lines possible due to reduced cell wall cross-links between cell wall biopolymers by PcGCE.
24997793	1	3	from	glucuronoxylan	322:335	arg1	lignin					359:364	lignin	359:364	lignin	359:364	The secondary walls of angiosperms contain large amounts of glucuronoxylan that is thought to be covalently linked to lignin via ester bonds between 4-O-methyl-α-D-glucuronic acid (4-O-Me-GlcA) moieties in glucuronoxylan and alcohol groups in lignin.
24997793	3	4	from	overexpression	496:509	arg1	carnosa					567:573	the white-rot basidiomycete Phanerochaete carnosa	525:573	the white-rot basidiomycete Phanerochaete carnosa	525:573	We report effects of overexpression of a GCE from the white-rot basidiomycete Phanerochaete carnosa, PcGCE, in hybrid aspen (Populus tremula L. x tremuloides Michx.)
24997793	3	4	from	overexpression	496:509	arg1	aspen					593:597	hybrid aspen	586:597	hybrid aspen	586:597	We report effects of overexpression of a GCE from the white-rot basidiomycete Phanerochaete carnosa, PcGCE, in hybrid aspen (Populus tremula L. x tremuloides Michx.)
24997793	3	4	from	overexpression	496:509	arg1	PcGCE					576:580	PcGCE	576:580	PcGCE	576:580	We report effects of overexpression of a GCE from the white-rot basidiomycete Phanerochaete carnosa, PcGCE, in hybrid aspen (Populus tremula L. x tremuloides Michx.)
24997793	1	5	theme	ester	245:249	arg1	bonds					251:255	ester bonds	245:255	ester bonds between 4-O-methyl-α-D-glucuronic acid (4-O-Me-GlcA) moieties in glucuronoxylan and alcohol groups in lignin	245:364	The secondary walls of angiosperms contain large amounts of glucuronoxylan that is thought to be covalently linked to lignin via ester bonds between 4-O-methyl-α-D-glucuronic acid (4-O-Me-GlcA) moieties in glucuronoxylan and alcohol groups in lignin.
24997793	10	6	theme	Glc	1943:1945	arg1	yields					1947:1952	Glc yields	1943:1952	Glc yields similar to wild-type	1943:1973	In contrast, the enzymatic saccharification after acid pretreatment resulted in Glc yields similar to wild-type despite of their lower cellulose content.
24997793	1	7	from	moieties	310:317	arg1	groups					349:354	alcohol groups	341:354	alcohol groups	341:354	The secondary walls of angiosperms contain large amounts of glucuronoxylan that is thought to be covalently linked to lignin via ester bonds between 4-O-methyl-α-D-glucuronic acid (4-O-Me-GlcA) moieties in glucuronoxylan and alcohol groups in lignin.
24997793	1	7	from	moieties	310:317	arg1	glucuronoxylan					322:335	glucuronoxylan	322:335	glucuronoxylan	322:335	The secondary walls of angiosperms contain large amounts of glucuronoxylan that is thought to be covalently linked to lignin via ester bonds between 4-O-methyl-α-D-glucuronic acid (4-O-Me-GlcA) moieties in glucuronoxylan and alcohol groups in lignin.
24997793	6	8	from	increase	1098:1105	arg1	content					1117:1123	lignin content	1110:1123	lignin content	1110:1123	Diffuse reflectance FT-IR spectroscopy and pyrolysis-GC/MS analyses showed significant alternation in wood chemistry of transgenic plants including an increase in lignin content and S/G ratio, and a decrease in carbohydrate content.
24997793	6	8	from	increase	1098:1105	arg1	ratio					1133:1137	S/G ratio	1129:1137	S/G ratio	1129:1137	Diffuse reflectance FT-IR spectroscopy and pyrolysis-GC/MS analyses showed significant alternation in wood chemistry of transgenic plants including an increase in lignin content and S/G ratio, and a decrease in carbohydrate content.
24997793	6	8	from	increase	1098:1105	arg1	content					1171:1177	carbohydrate content	1158:1177	carbohydrate content	1158:1177	Diffuse reflectance FT-IR spectroscopy and pyrolysis-GC/MS analyses showed significant alternation in wood chemistry of transgenic plants including an increase in lignin content and S/G ratio, and a decrease in carbohydrate content.
24997793	3	9	theme	basidiomycete	539:551	arg1	carnosa					567:573	the white-rot basidiomycete Phanerochaete carnosa	525:573	the white-rot basidiomycete Phanerochaete carnosa	525:573	We report effects of overexpression of a GCE from the white-rot basidiomycete Phanerochaete carnosa, PcGCE, in hybrid aspen (Populus tremula L. x tremuloides Michx.)
24997793	3	9	theme	basidiomycete	539:551	arg1	PcGCE					576:580	PcGCE	576:580	PcGCE	576:580	We report effects of overexpression of a GCE from the white-rot basidiomycete Phanerochaete carnosa, PcGCE, in hybrid aspen (Populus tremula L. x tremuloides Michx.)
24997793	7	10	dep	decrease	1331:1338	arg1	%					1329:1329	%	1329:1329	%	1329:1329	Sequential wood extractions confirmed a massive (+43%) increase of Klason lignin, which was accompanied by a ca. 5% decrease in cellulose, and ca. 20% decrease in wood extractives.
24997793	6	11	theme	carbohydrate	1158:1169	arg1	content					1171:1177	carbohydrate content	1158:1177	carbohydrate content	1158:1177	Diffuse reflectance FT-IR spectroscopy and pyrolysis-GC/MS analyses showed significant alternation in wood chemistry of transgenic plants including an increase in lignin content and S/G ratio, and a decrease in carbohydrate content.
24997793	4	12	theme	wood	648:651	arg1	composition					653:663	the wood composition	644:663	the wood composition	644:663	on the wood composition and the saccharification efficiency.
24997793	3	13	from	effects	485:491	arg1	aspen					593:597	hybrid aspen	586:597	hybrid aspen	586:597	We report effects of overexpression of a GCE from the white-rot basidiomycete Phanerochaete carnosa, PcGCE, in hybrid aspen (Populus tremula L. x tremuloides Michx.)
24997793	6	14	theme	reflectance	955:965	arg1	spectroscopy					973:984	Diffuse reflectance FT-IR spectroscopy	947:984	Diffuse reflectance FT-IR spectroscopy	947:984	Diffuse reflectance FT-IR spectroscopy and pyrolysis-GC/MS analyses showed significant alternation in wood chemistry of transgenic plants including an increase in lignin content and S/G ratio, and a decrease in carbohydrate content.
24997793	11	15	theme	possible	2317:2324	arg1	lines					2311:2315	the transgenic lines	2296:2315	the transgenic lines possible due to reduced cell wall cross-links between cell wall biopolymers by PcGCE	2296:2400	These data indicate that whereas PcGCE expression in hybrid aspen increases lignin deposition, the inhibitory effects of lignin are efficiently removed during acid pretreatment, and the extent of wood cellulose conversion during hydrolysis after acid pretreatment is improved in the transgenic lines possible due to reduced cell wall cross-links between cell wall biopolymers by PcGCE.
24997793	10	16	theme	similar	1954:1960	arg1	yields					1947:1952	Glc yields	1943:1952	Glc yields similar to wild-type	1943:1973	In contrast, the enzymatic saccharification after acid pretreatment resulted in Glc yields similar to wild-type despite of their lower cellulose content.
24997793	5	17	theme	cellulase	811:819	arg1	PttCel9B3					821:829	hybrid aspen cellulase PttCel9B3	798:829	hybrid aspen cellulase PttCel9B3	798:829	The recombinant enzyme, which was targeted to the plant cell wall using the signal peptide from hybrid aspen cellulase PttCel9B3, was constitutively expressed resulting in the appearance of GCE activity in protein extracts from developing wood.
24997793	11	18	theme	PcGCE	2050:2054	arg1	expression					2056:2065	PcGCE expression	2050:2065	PcGCE expression in hybrid aspen	2050:2081	These data indicate that whereas PcGCE expression in hybrid aspen increases lignin deposition, the inhibitory effects of lignin are efficiently removed during acid pretreatment, and the extent of wood cellulose conversion during hydrolysis after acid pretreatment is improved in the transgenic lines possible due to reduced cell wall cross-links between cell wall biopolymers by PcGCE.
24997793	11	19	theme	transgenic	2300:2309	arg1	lines					2311:2315	the transgenic lines	2296:2315	the transgenic lines possible due to reduced cell wall cross-links between cell wall biopolymers by PcGCE	2296:2400	These data indicate that whereas PcGCE expression in hybrid aspen increases lignin deposition, the inhibitory effects of lignin are efficiently removed during acid pretreatment, and the extent of wood cellulose conversion during hydrolysis after acid pretreatment is improved in the transgenic lines possible due to reduced cell wall cross-links between cell wall biopolymers by PcGCE.
24997793	10	20	theme	lower	1992:1996	arg1	content					2008:2014	their lower cellulose content	1986:2014	their lower cellulose content	1986:2014	In contrast, the enzymatic saccharification after acid pretreatment resulted in Glc yields similar to wild-type despite of their lower cellulose content.
24997793	5	21	theme	recombinant	706:716	arg1	enzyme					718:723	The recombinant enzyme	702:723	The recombinant enzyme	702:723	The recombinant enzyme, which was targeted to the plant cell wall using the signal peptide from hybrid aspen cellulase PttCel9B3, was constitutively expressed resulting in the appearance of GCE activity in protein extracts from developing wood.
24997793	8	22	from	change	1475:1480	arg1	contents					1489:1496	Xyl contents	1485:1496	Xyl contents in transgenic lines	1485:1516	Analysis of the monosaccharide composition using methanolysis showed a reduction of 4-O-Me-GlcA content without a change in Xyl contents in transgenic lines, suggesting that the covalent links between 4-O-Me-GlcA moieties and lignin protect these moieties from degradation.
24997793	5	23	from	PttCel9B3	821:829	arg1	peptide					785:791	the signal peptide	774:791	the signal peptide from hybrid aspen cellulase PttCel9B3	774:829	The recombinant enzyme, which was targeted to the plant cell wall using the signal peptide from hybrid aspen cellulase PttCel9B3, was constitutively expressed resulting in the appearance of GCE activity in protein extracts from developing wood.
24997793	7	24	dep	20	1327:1328	arg1	ca.					1323:1325	ca.	1323:1325	ca.	1323:1325	Sequential wood extractions confirmed a massive (+43%) increase of Klason lignin, which was accompanied by a ca. 5% decrease in cellulose, and ca. 20% decrease in wood extractives.
24997793	11	25	theme	lignin	2093:2098	arg1	deposition					2100:2109	lignin deposition	2093:2109	lignin deposition	2093:2109	These data indicate that whereas PcGCE expression in hybrid aspen increases lignin deposition, the inhibitory effects of lignin are efficiently removed during acid pretreatment, and the extent of wood cellulose conversion during hydrolysis after acid pretreatment is improved in the transgenic lines possible due to reduced cell wall cross-links between cell wall biopolymers by PcGCE.
24997793	5	26	theme	activity	896:903	arg1	appearance					878:887	the appearance	874:887	the appearance of GCE activity in protein extracts from developing wood	874:944	The recombinant enzyme, which was targeted to the plant cell wall using the signal peptide from hybrid aspen cellulase PttCel9B3, was constitutively expressed resulting in the appearance of GCE activity in protein extracts from developing wood.
24997793	3	27	from	carnosa	567:573	arg1	overexpression					496:509	overexpression	496:509	overexpression of a GCE from the white-rot basidiomycete Phanerochaete carnosa, PcGCE, in hybrid aspen	496:597	We report effects of overexpression of a GCE from the white-rot basidiomycete Phanerochaete carnosa, PcGCE, in hybrid aspen (Populus tremula L. x tremuloides Michx.)
24997793	3	27	from	carnosa	567:573	arg1	GCE					516:518	a GCE	514:518	a GCE from the white-rot basidiomycete Phanerochaete carnosa, PcGCE,	514:581	We report effects of overexpression of a GCE from the white-rot basidiomycete Phanerochaete carnosa, PcGCE, in hybrid aspen (Populus tremula L. x tremuloides Michx.)
24997793	5	28	theme	protein	908:914	arg1	extracts					916:923	protein extracts	908:923	protein extracts from developing wood	908:944	The recombinant enzyme, which was targeted to the plant cell wall using the signal peptide from hybrid aspen cellulase PttCel9B3, was constitutively expressed resulting in the appearance of GCE activity in protein extracts from developing wood.
24997793	1	29	theme	secondary	120:128	arg1	walls					130:134	The secondary walls	116:134	The secondary walls of angiosperms	116:149	The secondary walls of angiosperms contain large amounts of glucuronoxylan that is thought to be covalently linked to lignin via ester bonds between 4-O-methyl-α-D-glucuronic acid (4-O-Me-GlcA) moieties in glucuronoxylan and alcohol groups in lignin.
24997793	11	30	theme	cellulose	2218:2226	arg1	conversion					2228:2237	wood cellulose conversion	2213:2237	wood cellulose conversion during hydrolysis after acid pretreatment	2213:2279	These data indicate that whereas PcGCE expression in hybrid aspen increases lignin deposition, the inhibitory effects of lignin are efficiently removed during acid pretreatment, and the extent of wood cellulose conversion during hydrolysis after acid pretreatment is improved in the transgenic lines possible due to reduced cell wall cross-links between cell wall biopolymers by PcGCE.
24997793	8	31	theme	content	1457:1463	arg1	reduction					1432:1440	a reduction	1430:1440	a reduction of 4-O-Me-GlcA content	1430:1463	Analysis of the monosaccharide composition using methanolysis showed a reduction of 4-O-Me-GlcA content without a change in Xyl contents in transgenic lines, suggesting that the covalent links between 4-O-Me-GlcA moieties and lignin protect these moieties from degradation.
24997793	7	32	from	decrease	1331:1338	arg1	extractives					1348:1358	wood extractives	1343:1358	wood extractives	1343:1358	Sequential wood extractions confirmed a massive (+43%) increase of Klason lignin, which was accompanied by a ca. 5% decrease in cellulose, and ca. 20% decrease in wood extractives.
24997793	1	33	theme	angiosperms	139:149	arg1	walls					130:134	The secondary walls	116:134	The secondary walls of angiosperms	116:149	The secondary walls of angiosperms contain large amounts of glucuronoxylan that is thought to be covalently linked to lignin via ester bonds between 4-O-methyl-α-D-glucuronic acid (4-O-Me-GlcA) moieties in glucuronoxylan and alcohol groups in lignin.
24997793	9	34	from	Gal	1734:1736	arg1	lines					1770:1774	transgenic lines	1759:1774	transgenic lines	1759:1774	Enzymatic saccharification without pretreatment resulted in significant decreases of the yields of Gal, Glc, Xyl and Man in transgenic lines, consistent with their increased recalcitrance caused by the increased lignin content.
24997793	6	35	dep	spectroscopy	973:984	arg1	analyses					1006:1013	analyses	1006:1013	analyses	1006:1013	Diffuse reflectance FT-IR spectroscopy and pyrolysis-GC/MS analyses showed significant alternation in wood chemistry of transgenic plants including an increase in lignin content and S/G ratio, and a decrease in carbohydrate content.
24997793	4	36	theme	saccharification	673:688	arg1	efficiency					690:699	the saccharification efficiency	669:699	the saccharification efficiency	669:699	on the wood composition and the saccharification efficiency.
24997793	5	37	theme	plant	752:756	arg1	wall					763:766	the plant cell wall	748:766	the plant cell wall	748:766	The recombinant enzyme, which was targeted to the plant cell wall using the signal peptide from hybrid aspen cellulase PttCel9B3, was constitutively expressed resulting in the appearance of GCE activity in protein extracts from developing wood.
24997793	5	38	from	appearance	878:887	arg1	extracts					916:923	protein extracts	908:923	protein extracts from developing wood	908:944	The recombinant enzyme, which was targeted to the plant cell wall using the signal peptide from hybrid aspen cellulase PttCel9B3, was constitutively expressed resulting in the appearance of GCE activity in protein extracts from developing wood.
24997793	5	38	from	appearance	878:887	arg1	wood					941:944	developing wood	930:944	developing wood	930:944	The recombinant enzyme, which was targeted to the plant cell wall using the signal peptide from hybrid aspen cellulase PttCel9B3, was constitutively expressed resulting in the appearance of GCE activity in protein extracts from developing wood.
24997793	1	39	theme	large	159:163	arg1	glucuronoxylan					176:189	glucuronoxylan	176:189	glucuronoxylan	176:189	The secondary walls of angiosperms contain large amounts of glucuronoxylan that is thought to be covalently linked to lignin via ester bonds between 4-O-methyl-α-D-glucuronic acid (4-O-Me-GlcA) moieties in glucuronoxylan and alcohol groups in lignin.
24997793	1	39	theme	large	159:163	arg1	amounts					165:171	large amounts	159:171	large amounts of glucuronoxylan that is thought to be covalently linked to lignin via ester bonds between 4-O-methyl-α-D-glucuronic acid (4-O-Me-GlcA) moieties in glucuronoxylan and alcohol groups in lignin	159:364	The secondary walls of angiosperms contain large amounts of glucuronoxylan that is thought to be covalently linked to lignin via ester bonds between 4-O-methyl-α-D-glucuronic acid (4-O-Me-GlcA) moieties in glucuronoxylan and alcohol groups in lignin.
24997793	9	40	from	yields	1724:1729	arg1	lines					1770:1774	transgenic lines	1759:1774	transgenic lines	1759:1774	Enzymatic saccharification without pretreatment resulted in significant decreases of the yields of Gal, Glc, Xyl and Man in transgenic lines, consistent with their increased recalcitrance caused by the increased lignin content.
24997793	0	41	from	Expression	0:9	arg1	Populus					47:53	Populus	47:53	Populus	47:53	Expression of a fungal glucuronoyl esterase in Populus: effects on wood properties and saccharification efficiency.
24997793	7	42	theme	massive	1220:1226	arg1	increase					1235:1242	a massive (+43%) increase	1218:1242	a massive (+43%) increase of Klason lignin, which was accompanied by a ca. 5% decrease in cellulose,	1218:1317	Sequential wood extractions confirmed a massive (+43%) increase of Klason lignin, which was accompanied by a ca. 5% decrease in cellulose, and ca. 20% decrease in wood extractives.
24997793	7	42	theme	massive	1220:1226	arg1	%					1232:1232	+43%	1229:1232	+43%	1229:1232	Sequential wood extractions confirmed a massive (+43%) increase of Klason lignin, which was accompanied by a ca. 5% decrease in cellulose, and ca. 20% decrease in wood extractives.
24997793	3	43	dep	report	478:483	arg1	Michx					633:637	Michx	633:637	Michx	633:637	We report effects of overexpression of a GCE from the white-rot basidiomycete Phanerochaete carnosa, PcGCE, in hybrid aspen (Populus tremula L. x tremuloides Michx.)
24997793	3	43	dep	report	478:483	arg1	L.					616:617	L.	616:617	L.	616:617	We report effects of overexpression of a GCE from the white-rot basidiomycete Phanerochaete carnosa, PcGCE, in hybrid aspen (Populus tremula L. x tremuloides Michx.)
24997793	0	44	theme	saccharification	87:102	arg1	efficiency					104:113	saccharification efficiency	87:113	saccharification efficiency	87:113	Expression of a fungal glucuronoyl esterase in Populus: effects on wood properties and saccharification efficiency.
24997793	0	45	theme	esterase	35:42	arg1	Expression					0:9	Expression	0:9	Expression of a fungal glucuronoyl esterase in Populus: effects on wood properties and saccharification efficiency.	0:114	Expression of a fungal glucuronoyl esterase in Populus: effects on wood properties and saccharification efficiency.
24997793	9	46	from	Glc	1739:1741	arg1	lines					1770:1774	transgenic lines	1759:1774	transgenic lines	1759:1774	Enzymatic saccharification without pretreatment resulted in significant decreases of the yields of Gal, Glc, Xyl and Man in transgenic lines, consistent with their increased recalcitrance caused by the increased lignin content.
24997793	11	47	theme	acid	2176:2179	arg1	pretreatment					2181:2192	acid pretreatment	2176:2192	acid pretreatment	2176:2192	These data indicate that whereas PcGCE expression in hybrid aspen increases lignin deposition, the inhibitory effects of lignin are efficiently removed during acid pretreatment, and the extent of wood cellulose conversion during hydrolysis after acid pretreatment is improved in the transgenic lines possible due to reduced cell wall cross-links between cell wall biopolymers by PcGCE.
24997793	8	48	theme	transgenic	1501:1510	arg1	lines					1512:1516	transgenic lines	1501:1516	transgenic lines	1501:1516	Analysis of the monosaccharide composition using methanolysis showed a reduction of 4-O-Me-GlcA content without a change in Xyl contents in transgenic lines, suggesting that the covalent links between 4-O-Me-GlcA moieties and lignin protect these moieties from degradation.
24997793	7	49	theme	wood	1191:1194	arg1	extractions					1196:1206	Sequential wood extractions	1180:1206	Sequential wood extractions	1180:1206	Sequential wood extractions confirmed a massive (+43%) increase of Klason lignin, which was accompanied by a ca. 5% decrease in cellulose, and ca. 20% decrease in wood extractives.
24997793	1	50	theme	4-O-methyl-α-D-glucuronic	265:289	arg1	moieties					310:317	4-O-methyl-α-D-glucuronic acid (4-O-Me-GlcA) moieties	265:317	4-O-methyl-α-D-glucuronic acid (4-O-Me-GlcA) moieties in glucuronoxylan and alcohol groups in lignin	265:364	The secondary walls of angiosperms contain large amounts of glucuronoxylan that is thought to be covalently linked to lignin via ester bonds between 4-O-methyl-α-D-glucuronic acid (4-O-Me-GlcA) moieties in glucuronoxylan and alcohol groups in lignin.
24997793	6	51	theme	plants	1078:1083	arg1	chemistry					1054:1062	wood chemistry	1049:1062	wood chemistry of transgenic plants including an increase in lignin content and S/G ratio, and a decrease in carbohydrate content	1049:1177	Diffuse reflectance FT-IR spectroscopy and pyrolysis-GC/MS analyses showed significant alternation in wood chemistry of transgenic plants including an increase in lignin content and S/G ratio, and a decrease in carbohydrate content.
24997793	5	52	theme	hybrid	798:803	arg1	PttCel9B3					821:829	hybrid aspen cellulase PttCel9B3	798:829	hybrid aspen cellulase PttCel9B3	798:829	The recombinant enzyme, which was targeted to the plant cell wall using the signal peptide from hybrid aspen cellulase PttCel9B3, was constitutively expressed resulting in the appearance of GCE activity in protein extracts from developing wood.
24997793	9	53	from	lines	1770:1774	arg1	yields					1724:1729	the yields	1720:1729	the yields of Gal, Glc, Xyl and Man in transgenic lines	1720:1774	Enzymatic saccharification without pretreatment resulted in significant decreases of the yields of Gal, Glc, Xyl and Man in transgenic lines, consistent with their increased recalcitrance caused by the increased lignin content.
24997793	3	54	from	aspen	593:597	arg1	effects					485:491	effects	485:491	effects of overexpression of a GCE from the white-rot basidiomycete Phanerochaete carnosa, PcGCE, in hybrid aspen	485:597	We report effects of overexpression of a GCE from the white-rot basidiomycete Phanerochaete carnosa, PcGCE, in hybrid aspen (Populus tremula L. x tremuloides Michx.)
24997793	0	55	theme	glucuronoyl	23:33	arg1	esterase					35:42	a fungal glucuronoyl esterase	14:42	a fungal glucuronoyl esterase	14:42	Expression of a fungal glucuronoyl esterase in Populus: effects on wood properties and saccharification efficiency.
24997793	7	56	dep	decrease	1296:1303	arg1	%					1294:1294	%	1294:1294	%	1294:1294	Sequential wood extractions confirmed a massive (+43%) increase of Klason lignin, which was accompanied by a ca. 5% decrease in cellulose, and ca. 20% decrease in wood extractives.
24997793	6	57	theme	wood	1049:1052	arg1	chemistry					1054:1062	wood chemistry	1049:1062	wood chemistry of transgenic plants including an increase in lignin content and S/G ratio, and a decrease in carbohydrate content	1049:1177	Diffuse reflectance FT-IR spectroscopy and pyrolysis-GC/MS analyses showed significant alternation in wood chemistry of transgenic plants including an increase in lignin content and S/G ratio, and a decrease in carbohydrate content.
24997793	2	58	theme	wood-degrading	453:466	arg1	fungi					468:472	wood-degrading fungi	453:472	wood-degrading fungi	453:472	This linkage is proposed to be hydrolysed by glucuronoyl esterases (GCEs) secreted by wood-degrading fungi.
24997793	8	59	theme	covalent	1539:1546	arg1	links					1548:1552	the covalent links	1535:1552	the covalent links between 4-O-Me-GlcA moieties and lignin	1535:1592	Analysis of the monosaccharide composition using methanolysis showed a reduction of 4-O-Me-GlcA content without a change in Xyl contents in transgenic lines, suggesting that the covalent links between 4-O-Me-GlcA moieties and lignin protect these moieties from degradation.
24997793	11	60	theme	conversion	2228:2237	arg1	extent					2203:2208	the extent	2199:2208	the extent of wood cellulose conversion during hydrolysis after acid pretreatment	2199:2279	These data indicate that whereas PcGCE expression in hybrid aspen increases lignin deposition, the inhibitory effects of lignin are efficiently removed during acid pretreatment, and the extent of wood cellulose conversion during hydrolysis after acid pretreatment is improved in the transgenic lines possible due to reduced cell wall cross-links between cell wall biopolymers by PcGCE.
24997793	11	61	theme	lignin	2138:2143	arg1	effects					2127:2133	the inhibitory effects	2112:2133	the inhibitory effects of lignin	2112:2143	These data indicate that whereas PcGCE expression in hybrid aspen increases lignin deposition, the inhibitory effects of lignin are efficiently removed during acid pretreatment, and the extent of wood cellulose conversion during hydrolysis after acid pretreatment is improved in the transgenic lines possible due to reduced cell wall cross-links between cell wall biopolymers by PcGCE.
24997793	8	62	from	contents	1489:1496	arg1	lines					1512:1516	transgenic lines	1501:1516	transgenic lines	1501:1516	Analysis of the monosaccharide composition using methanolysis showed a reduction of 4-O-Me-GlcA content without a change in Xyl contents in transgenic lines, suggesting that the covalent links between 4-O-Me-GlcA moieties and lignin protect these moieties from degradation.
24997793	9	63	theme	increased	1837:1845	arg1	content					1854:1860	the increased lignin content	1833:1860	the increased lignin content	1833:1860	Enzymatic saccharification without pretreatment resulted in significant decreases of the yields of Gal, Glc, Xyl and Man in transgenic lines, consistent with their increased recalcitrance caused by the increased lignin content.
24997793	8	64	theme	monosaccharide	1377:1390	arg1	composition					1392:1402	the monosaccharide composition	1373:1402	the monosaccharide composition using methanolysis	1373:1421	Analysis of the monosaccharide composition using methanolysis showed a reduction of 4-O-Me-GlcA content without a change in Xyl contents in transgenic lines, suggesting that the covalent links between 4-O-Me-GlcA moieties and lignin protect these moieties from degradation.
24997793	5	65	from	wood	941:944	arg1	appearance					878:887	the appearance	874:887	the appearance of GCE activity in protein extracts from developing wood	874:944	The recombinant enzyme, which was targeted to the plant cell wall using the signal peptide from hybrid aspen cellulase PttCel9B3, was constitutively expressed resulting in the appearance of GCE activity in protein extracts from developing wood.
24997793	5	65	from	wood	941:944	arg1	extracts					916:923	protein extracts	908:923	protein extracts from developing wood	908:944	The recombinant enzyme, which was targeted to the plant cell wall using the signal peptide from hybrid aspen cellulase PttCel9B3, was constitutively expressed resulting in the appearance of GCE activity in protein extracts from developing wood.
24997793	6	66	theme	S/G	1129:1131	arg1	ratio					1133:1137	S/G ratio	1129:1137	S/G ratio	1129:1137	Diffuse reflectance FT-IR spectroscopy and pyrolysis-GC/MS analyses showed significant alternation in wood chemistry of transgenic plants including an increase in lignin content and S/G ratio, and a decrease in carbohydrate content.
24997793	7	67	dep	5	1293:1293	arg1	ca.					1289:1291	ca.	1289:1291	ca.	1289:1291	Sequential wood extractions confirmed a massive (+43%) increase of Klason lignin, which was accompanied by a ca. 5% decrease in cellulose, and ca. 20% decrease in wood extractives.
24997793	7	68	theme	lignin	1254:1259	arg1	decrease					1331:1338	ca. 20% decrease	1323:1338	ca. 20% decrease in wood extractives	1323:1358	Sequential wood extractions confirmed a massive (+43%) increase of Klason lignin, which was accompanied by a ca. 5% decrease in cellulose, and ca. 20% decrease in wood extractives.
24997793	7	68	theme	lignin	1254:1259	arg1	increase					1235:1242	a massive (+43%) increase	1218:1242	a massive (+43%) increase of Klason lignin, which was accompanied by a ca. 5% decrease in cellulose,	1218:1317	Sequential wood extractions confirmed a massive (+43%) increase of Klason lignin, which was accompanied by a ca. 5% decrease in cellulose, and ca. 20% decrease in wood extractives.
24997793	7	68	theme	lignin	1254:1259	arg1	%					1232:1232	+43%	1229:1232	+43%	1229:1232	Sequential wood extractions confirmed a massive (+43%) increase of Klason lignin, which was accompanied by a ca. 5% decrease in cellulose, and ca. 20% decrease in wood extractives.
24997793	3	69	theme	overexpression	496:509	arg1	effects					485:491	effects	485:491	effects of overexpression of a GCE from the white-rot basidiomycete Phanerochaete carnosa, PcGCE, in hybrid aspen	485:597	We report effects of overexpression of a GCE from the white-rot basidiomycete Phanerochaete carnosa, PcGCE, in hybrid aspen (Populus tremula L. x tremuloides Michx.)
24997793	9	70	theme	Enzymatic	1635:1643	arg1	saccharification					1645:1660	Enzymatic saccharification	1635:1660	Enzymatic saccharification without pretreatment	1635:1681	Enzymatic saccharification without pretreatment resulted in significant decreases of the yields of Gal, Glc, Xyl and Man in transgenic lines, consistent with their increased recalcitrance caused by the increased lignin content.
24997793	7	71	from	increase	1235:1242	arg1	extractives					1348:1358	wood extractives	1343:1358	wood extractives	1343:1358	Sequential wood extractions confirmed a massive (+43%) increase of Klason lignin, which was accompanied by a ca. 5% decrease in cellulose, and ca. 20% decrease in wood extractives.
24997793	11	72	theme	wall	2346:2349	arg1	cross-links					2351:2361	reduced cell wall cross-links	2333:2361	reduced cell wall cross-links between cell wall biopolymers by PcGCE	2333:2400	These data indicate that whereas PcGCE expression in hybrid aspen increases lignin deposition, the inhibitory effects of lignin are efficiently removed during acid pretreatment, and the extent of wood cellulose conversion during hydrolysis after acid pretreatment is improved in the transgenic lines possible due to reduced cell wall cross-links between cell wall biopolymers by PcGCE.
24997793	10	73	theme	enzymatic	1880:1888	arg1	saccharification					1890:1905	the enzymatic saccharification	1876:1905	the enzymatic saccharification after acid pretreatment	1876:1929	In contrast, the enzymatic saccharification after acid pretreatment resulted in Glc yields similar to wild-type despite of their lower cellulose content.
24997793	3	74	theme	white-rot	529:537	arg1	carnosa					567:573	the white-rot basidiomycete Phanerochaete carnosa	525:573	the white-rot basidiomycete Phanerochaete carnosa	525:573	We report effects of overexpression of a GCE from the white-rot basidiomycete Phanerochaete carnosa, PcGCE, in hybrid aspen (Populus tremula L. x tremuloides Michx.)
24997793	3	74	theme	white-rot	529:537	arg1	PcGCE					576:580	PcGCE	576:580	PcGCE	576:580	We report effects of overexpression of a GCE from the white-rot basidiomycete Phanerochaete carnosa, PcGCE, in hybrid aspen (Populus tremula L. x tremuloides Michx.)
24997793	1	75	contain	contain	151:157	arg2	amounts					165:171	large amounts	159:171	large amounts of glucuronoxylan that is thought to be covalently linked to lignin via ester bonds between 4-O-methyl-α-D-glucuronic acid (4-O-Me-GlcA) moieties in glucuronoxylan and alcohol groups in lignin	159:364	The secondary walls of angiosperms contain large amounts of glucuronoxylan that is thought to be covalently linked to lignin via ester bonds between 4-O-methyl-α-D-glucuronic acid (4-O-Me-GlcA) moieties in glucuronoxylan and alcohol groups in lignin.
24997793	1	75	contain	contain	151:157	arg1	walls					130:134	The secondary walls	116:134	The secondary walls of angiosperms	116:149	The secondary walls of angiosperms contain large amounts of glucuronoxylan that is thought to be covalently linked to lignin via ester bonds between 4-O-methyl-α-D-glucuronic acid (4-O-Me-GlcA) moieties in glucuronoxylan and alcohol groups in lignin.
24997793	1	75	contain	contain	151:157	arg2	glucuronoxylan					176:189	glucuronoxylan	176:189	glucuronoxylan	176:189	The secondary walls of angiosperms contain large amounts of glucuronoxylan that is thought to be covalently linked to lignin via ester bonds between 4-O-methyl-α-D-glucuronic acid (4-O-Me-GlcA) moieties in glucuronoxylan and alcohol groups in lignin.
24997793	7	76	dep	%	1329:1329	arg1	20					1327:1328	20	1327:1328	20	1327:1328	Sequential wood extractions confirmed a massive (+43%) increase of Klason lignin, which was accompanied by a ca. 5% decrease in cellulose, and ca. 20% decrease in wood extractives.
24997793	11	77	theme	wood	2213:2216	arg1	conversion					2228:2237	wood cellulose conversion	2213:2237	wood cellulose conversion during hydrolysis after acid pretreatment	2213:2279	These data indicate that whereas PcGCE expression in hybrid aspen increases lignin deposition, the inhibitory effects of lignin are efficiently removed during acid pretreatment, and the extent of wood cellulose conversion during hydrolysis after acid pretreatment is improved in the transgenic lines possible due to reduced cell wall cross-links between cell wall biopolymers by PcGCE.
24997793	1	78	theme	glucuronoxylan	176:189	arg1	glucuronoxylan					176:189	glucuronoxylan	176:189	glucuronoxylan	176:189	The secondary walls of angiosperms contain large amounts of glucuronoxylan that is thought to be covalently linked to lignin via ester bonds between 4-O-methyl-α-D-glucuronic acid (4-O-Me-GlcA) moieties in glucuronoxylan and alcohol groups in lignin.
24997793	1	78	theme	glucuronoxylan	176:189	arg1	amounts					165:171	large amounts	159:171	large amounts of glucuronoxylan that is thought to be covalently linked to lignin via ester bonds between 4-O-methyl-α-D-glucuronic acid (4-O-Me-GlcA) moieties in glucuronoxylan and alcohol groups in lignin	159:364	The secondary walls of angiosperms contain large amounts of glucuronoxylan that is thought to be covalently linked to lignin via ester bonds between 4-O-methyl-α-D-glucuronic acid (4-O-Me-GlcA) moieties in glucuronoxylan and alcohol groups in lignin.
24997793	6	79	theme	FT-IR	967:971	arg1	spectroscopy					973:984	Diffuse reflectance FT-IR spectroscopy	947:984	Diffuse reflectance FT-IR spectroscopy	947:984	Diffuse reflectance FT-IR spectroscopy and pyrolysis-GC/MS analyses showed significant alternation in wood chemistry of transgenic plants including an increase in lignin content and S/G ratio, and a decrease in carbohydrate content.
24997793	9	80	theme	significant	1695:1705	arg1	decreases					1707:1715	significant decreases	1695:1715	significant decreases of the yields of Gal, Glc, Xyl and Man in transgenic lines	1695:1774	Enzymatic saccharification without pretreatment resulted in significant decreases of the yields of Gal, Glc, Xyl and Man in transgenic lines, consistent with their increased recalcitrance caused by the increased lignin content.
24997793	3	81	theme	Phanerochaete	553:565	arg1	carnosa					567:573	the white-rot basidiomycete Phanerochaete carnosa	525:573	the white-rot basidiomycete Phanerochaete carnosa	525:573	We report effects of overexpression of a GCE from the white-rot basidiomycete Phanerochaete carnosa, PcGCE, in hybrid aspen (Populus tremula L. x tremuloides Michx.)
24997793	3	81	theme	Phanerochaete	553:565	arg1	PcGCE					576:580	PcGCE	576:580	PcGCE	576:580	We report effects of overexpression of a GCE from the white-rot basidiomycete Phanerochaete carnosa, PcGCE, in hybrid aspen (Populus tremula L. x tremuloides Michx.)
24997793	5	82	theme	aspen	805:809	arg1	PttCel9B3					821:829	hybrid aspen cellulase PttCel9B3	798:829	hybrid aspen cellulase PttCel9B3	798:829	The recombinant enzyme, which was targeted to the plant cell wall using the signal peptide from hybrid aspen cellulase PttCel9B3, was constitutively expressed resulting in the appearance of GCE activity in protein extracts from developing wood.
24997793	6	83	theme	Diffuse	947:953	arg1	spectroscopy					973:984	Diffuse reflectance FT-IR spectroscopy	947:984	Diffuse reflectance FT-IR spectroscopy	947:984	Diffuse reflectance FT-IR spectroscopy and pyrolysis-GC/MS analyses showed significant alternation in wood chemistry of transgenic plants including an increase in lignin content and S/G ratio, and a decrease in carbohydrate content.
24997793	11	84	theme	hybrid	2070:2075	arg1	aspen					2077:2081	hybrid aspen	2070:2081	hybrid aspen	2070:2081	These data indicate that whereas PcGCE expression in hybrid aspen increases lignin deposition, the inhibitory effects of lignin are efficiently removed during acid pretreatment, and the extent of wood cellulose conversion during hydrolysis after acid pretreatment is improved in the transgenic lines possible due to reduced cell wall cross-links between cell wall biopolymers by PcGCE.
24997793	11	85	theme	acid	2263:2266	arg1	pretreatment					2268:2279	acid pretreatment	2263:2279	acid pretreatment	2263:2279	These data indicate that whereas PcGCE expression in hybrid aspen increases lignin deposition, the inhibitory effects of lignin are efficiently removed during acid pretreatment, and the extent of wood cellulose conversion during hydrolysis after acid pretreatment is improved in the transgenic lines possible due to reduced cell wall cross-links between cell wall biopolymers by PcGCE.
24997793	3	86	theme	hybrid	586:591	arg1	aspen					593:597	hybrid aspen	586:597	hybrid aspen	586:597	We report effects of overexpression of a GCE from the white-rot basidiomycete Phanerochaete carnosa, PcGCE, in hybrid aspen (Populus tremula L. x tremuloides Michx.)
24997793	11	87	theme	reduced	2333:2339	arg1	cross-links					2351:2361	reduced cell wall cross-links	2333:2361	reduced cell wall cross-links between cell wall biopolymers by PcGCE	2333:2400	These data indicate that whereas PcGCE expression in hybrid aspen increases lignin deposition, the inhibitory effects of lignin are efficiently removed during acid pretreatment, and the extent of wood cellulose conversion during hydrolysis after acid pretreatment is improved in the transgenic lines possible due to reduced cell wall cross-links between cell wall biopolymers by PcGCE.
24997793	9	88	theme	yields	1724:1729	arg1	decreases					1707:1715	significant decreases	1695:1715	significant decreases of the yields of Gal, Glc, Xyl and Man in transgenic lines	1695:1774	Enzymatic saccharification without pretreatment resulted in significant decreases of the yields of Gal, Glc, Xyl and Man in transgenic lines, consistent with their increased recalcitrance caused by the increased lignin content.
24997793	0	89	from	effects	56:62	arg1	properties					72:81	wood properties	67:81	wood properties	67:81	Expression of a fungal glucuronoyl esterase in Populus: effects on wood properties and saccharification efficiency.
24997793	0	89	from	effects	56:62	arg1	efficiency					104:113	saccharification efficiency	87:113	saccharification efficiency	87:113	Expression of a fungal glucuronoyl esterase in Populus: effects on wood properties and saccharification efficiency.
24997793	7	90	theme	wood	1343:1346	arg1	extractives					1348:1358	wood extractives	1343:1358	wood extractives	1343:1358	Sequential wood extractions confirmed a massive (+43%) increase of Klason lignin, which was accompanied by a ca. 5% decrease in cellulose, and ca. 20% decrease in wood extractives.
24997793	5	91	theme	GCE	892:894	arg1	activity					896:903	GCE activity	892:903	GCE activity	892:903	The recombinant enzyme, which was targeted to the plant cell wall using the signal peptide from hybrid aspen cellulase PttCel9B3, was constitutively expressed resulting in the appearance of GCE activity in protein extracts from developing wood.
24997793	9	92	theme	Gal	1734:1736	arg1	yields					1724:1729	the yields	1720:1729	the yields of Gal, Glc, Xyl and Man in transgenic lines	1720:1774	Enzymatic saccharification without pretreatment resulted in significant decreases of the yields of Gal, Glc, Xyl and Man in transgenic lines, consistent with their increased recalcitrance caused by the increased lignin content.
24997793	8	93	theme	4-O-Me-GlcA	1445:1455	arg1	content					1457:1463	4-O-Me-GlcA content	1445:1463	4-O-Me-GlcA content	1445:1463	Analysis of the monosaccharide composition using methanolysis showed a reduction of 4-O-Me-GlcA content without a change in Xyl contents in transgenic lines, suggesting that the covalent links between 4-O-Me-GlcA moieties and lignin protect these moieties from degradation.
24997793	1	94	from	groups	349:354	arg1	lignin					359:364	lignin	359:364	lignin	359:364	The secondary walls of angiosperms contain large amounts of glucuronoxylan that is thought to be covalently linked to lignin via ester bonds between 4-O-methyl-α-D-glucuronic acid (4-O-Me-GlcA) moieties in glucuronoxylan and alcohol groups in lignin.
24997793	9	95	with	consistent	1777:1786	arg1	recalcitrance					1809:1821	their increased recalcitrance	1793:1821	their increased recalcitrance caused by the increased lignin content	1793:1860	Enzymatic saccharification without pretreatment resulted in significant decreases of the yields of Gal, Glc, Xyl and Man in transgenic lines, consistent with their increased recalcitrance caused by the increased lignin content.
24997793	9	96	theme	Glc	1739:1741	arg1	yields					1724:1729	the yields	1720:1729	the yields of Gal, Glc, Xyl and Man in transgenic lines	1720:1774	Enzymatic saccharification without pretreatment resulted in significant decreases of the yields of Gal, Glc, Xyl and Man in transgenic lines, consistent with their increased recalcitrance caused by the increased lignin content.
24997793	11	97	theme	cell	2341:2344	arg1	cross-links					2351:2361	reduced cell wall cross-links	2333:2361	reduced cell wall cross-links between cell wall biopolymers by PcGCE	2333:2400	These data indicate that whereas PcGCE expression in hybrid aspen increases lignin deposition, the inhibitory effects of lignin are efficiently removed during acid pretreatment, and the extent of wood cellulose conversion during hydrolysis after acid pretreatment is improved in the transgenic lines possible due to reduced cell wall cross-links between cell wall biopolymers by PcGCE.
24997793	6	98	theme	significant	1022:1032	arg1	alternation					1034:1044	significant alternation	1022:1044	significant alternation in wood chemistry of transgenic plants including an increase in lignin content and S/G ratio, and a decrease in carbohydrate content	1022:1177	Diffuse reflectance FT-IR spectroscopy and pyrolysis-GC/MS analyses showed significant alternation in wood chemistry of transgenic plants including an increase in lignin content and S/G ratio, and a decrease in carbohydrate content.
24997793	9	99	theme	Xyl	1744:1746	arg1	yields					1724:1729	the yields	1720:1729	the yields of Gal, Glc, Xyl and Man in transgenic lines	1720:1774	Enzymatic saccharification without pretreatment resulted in significant decreases of the yields of Gal, Glc, Xyl and Man in transgenic lines, consistent with their increased recalcitrance caused by the increased lignin content.
24997793	10	100	theme	cellulose	1998:2006	arg1	content					2008:2014	their lower cellulose content	1986:2014	their lower cellulose content	1986:2014	In contrast, the enzymatic saccharification after acid pretreatment resulted in Glc yields similar to wild-type despite of their lower cellulose content.
24997793	0	101	theme	wood	67:70	arg1	properties					72:81	wood properties	67:81	wood properties	67:81	Expression of a fungal glucuronoyl esterase in Populus: effects on wood properties and saccharification efficiency.
24997793	1	102	theme	alcohol	341:347	arg1	groups					349:354	alcohol groups	341:354	alcohol groups	341:354	The secondary walls of angiosperms contain large amounts of glucuronoxylan that is thought to be covalently linked to lignin via ester bonds between 4-O-methyl-α-D-glucuronic acid (4-O-Me-GlcA) moieties in glucuronoxylan and alcohol groups in lignin.
24997793	9	103	theme	Man	1752:1754	arg1	yields					1724:1729	the yields	1720:1729	the yields of Gal, Glc, Xyl and Man in transgenic lines	1720:1774	Enzymatic saccharification without pretreatment resulted in significant decreases of the yields of Gal, Glc, Xyl and Man in transgenic lines, consistent with their increased recalcitrance caused by the increased lignin content.
24997793	8	104	theme	Xyl	1485:1487	arg1	contents					1489:1496	Xyl contents	1485:1496	Xyl contents in transgenic lines	1485:1516	Analysis of the monosaccharide composition using methanolysis showed a reduction of 4-O-Me-GlcA content without a change in Xyl contents in transgenic lines, suggesting that the covalent links between 4-O-Me-GlcA moieties and lignin protect these moieties from degradation.
24997793	11	105	from	expression	2056:2065	arg1	aspen					2077:2081	hybrid aspen	2070:2081	hybrid aspen	2070:2081	These data indicate that whereas PcGCE expression in hybrid aspen increases lignin deposition, the inhibitory effects of lignin are efficiently removed during acid pretreatment, and the extent of wood cellulose conversion during hydrolysis after acid pretreatment is improved in the transgenic lines possible due to reduced cell wall cross-links between cell wall biopolymers by PcGCE.
24997793	9	106	theme	transgenic	1759:1768	arg1	lines					1770:1774	transgenic lines	1759:1774	transgenic lines	1759:1774	Enzymatic saccharification without pretreatment resulted in significant decreases of the yields of Gal, Glc, Xyl and Man in transgenic lines, consistent with their increased recalcitrance caused by the increased lignin content.
24997793	6	107	from	alternation	1034:1044	arg1	chemistry					1054:1062	wood chemistry	1049:1062	wood chemistry of transgenic plants including an increase in lignin content and S/G ratio, and a decrease in carbohydrate content	1049:1177	Diffuse reflectance FT-IR spectroscopy and pyrolysis-GC/MS analyses showed significant alternation in wood chemistry of transgenic plants including an increase in lignin content and S/G ratio, and a decrease in carbohydrate content.
24997793	11	108	theme	wall	2376:2379	arg1	biopolymers					2381:2391	cell wall biopolymers	2371:2391	cell wall biopolymers by PcGCE	2371:2400	These data indicate that whereas PcGCE expression in hybrid aspen increases lignin deposition, the inhibitory effects of lignin are efficiently removed during acid pretreatment, and the extent of wood cellulose conversion during hydrolysis after acid pretreatment is improved in the transgenic lines possible due to reduced cell wall cross-links between cell wall biopolymers by PcGCE.
24997793	5	109	theme	signal	778:783	arg1	peptide					785:791	the signal peptide	774:791	the signal peptide from hybrid aspen cellulase PttCel9B3	774:829	The recombinant enzyme, which was targeted to the plant cell wall using the signal peptide from hybrid aspen cellulase PttCel9B3, was constitutively expressed resulting in the appearance of GCE activity in protein extracts from developing wood.
24997793	6	110	from	decrease	1146:1153	arg1	content					1117:1123	lignin content	1110:1123	lignin content	1110:1123	Diffuse reflectance FT-IR spectroscopy and pyrolysis-GC/MS analyses showed significant alternation in wood chemistry of transgenic plants including an increase in lignin content and S/G ratio, and a decrease in carbohydrate content.
24997793	6	110	from	decrease	1146:1153	arg1	ratio					1133:1137	S/G ratio	1129:1137	S/G ratio	1129:1137	Diffuse reflectance FT-IR spectroscopy and pyrolysis-GC/MS analyses showed significant alternation in wood chemistry of transgenic plants including an increase in lignin content and S/G ratio, and a decrease in carbohydrate content.
24997793	6	110	from	decrease	1146:1153	arg1	content					1171:1177	carbohydrate content	1158:1177	carbohydrate content	1158:1177	Diffuse reflectance FT-IR spectroscopy and pyrolysis-GC/MS analyses showed significant alternation in wood chemistry of transgenic plants including an increase in lignin content and S/G ratio, and a decrease in carbohydrate content.
24997793	0	111	theme	fungal	16:21	arg1	esterase					35:42	a fungal glucuronoyl esterase	14:42	a fungal glucuronoyl esterase	14:42	Expression of a fungal glucuronoyl esterase in Populus: effects on wood properties and saccharification efficiency.
24997793	1	112	theme	acid	291:294	arg1	moieties					310:317	4-O-methyl-α-D-glucuronic acid (4-O-Me-GlcA) moieties	265:317	4-O-methyl-α-D-glucuronic acid (4-O-Me-GlcA) moieties in glucuronoxylan and alcohol groups in lignin	265:364	The secondary walls of angiosperms contain large amounts of glucuronoxylan that is thought to be covalently linked to lignin via ester bonds between 4-O-methyl-α-D-glucuronic acid (4-O-Me-GlcA) moieties in glucuronoxylan and alcohol groups in lignin.
24997793	5	113	theme	developing	930:939	arg1	wood					941:944	developing wood	930:944	developing wood	930:944	The recombinant enzyme, which was targeted to the plant cell wall using the signal peptide from hybrid aspen cellulase PttCel9B3, was constitutively expressed resulting in the appearance of GCE activity in protein extracts from developing wood.
24997793	1	114	attach	linked	224:229	arg2	amounts					165:171	large amounts	159:171	large amounts of glucuronoxylan that is thought to be covalently linked to lignin via ester bonds between 4-O-methyl-α-D-glucuronic acid (4-O-Me-GlcA) moieties in glucuronoxylan and alcohol groups in lignin	159:364	The secondary walls of angiosperms contain large amounts of glucuronoxylan that is thought to be covalently linked to lignin via ester bonds between 4-O-methyl-α-D-glucuronic acid (4-O-Me-GlcA) moieties in glucuronoxylan and alcohol groups in lignin.
24997793	1	114	attach	linked	224:229	arg1	lignin					234:239	lignin	234:239	lignin	234:239	The secondary walls of angiosperms contain large amounts of glucuronoxylan that is thought to be covalently linked to lignin via ester bonds between 4-O-methyl-α-D-glucuronic acid (4-O-Me-GlcA) moieties in glucuronoxylan and alcohol groups in lignin.
24997793	1	114	attach	linked	224:229	arg3	bonds					251:255	ester bonds	245:255	ester bonds between 4-O-methyl-α-D-glucuronic acid (4-O-Me-GlcA) moieties in glucuronoxylan and alcohol groups in lignin	245:364	The secondary walls of angiosperms contain large amounts of glucuronoxylan that is thought to be covalently linked to lignin via ester bonds between 4-O-methyl-α-D-glucuronic acid (4-O-Me-GlcA) moieties in glucuronoxylan and alcohol groups in lignin.
24997793	1	114	attach	linked	224:229	arg2	glucuronoxylan					176:189	glucuronoxylan	176:189	glucuronoxylan	176:189	The secondary walls of angiosperms contain large amounts of glucuronoxylan that is thought to be covalently linked to lignin via ester bonds between 4-O-methyl-α-D-glucuronic acid (4-O-Me-GlcA) moieties in glucuronoxylan and alcohol groups in lignin.
24997793	6	115	theme	transgenic	1067:1076	arg1	decrease					1146:1153	a decrease	1144:1153	a decrease in carbohydrate content	1144:1177	Diffuse reflectance FT-IR spectroscopy and pyrolysis-GC/MS analyses showed significant alternation in wood chemistry of transgenic plants including an increase in lignin content and S/G ratio, and a decrease in carbohydrate content.
24997793	6	115	theme	transgenic	1067:1076	arg1	plants					1078:1083	transgenic plants	1067:1083	transgenic plants including an increase in lignin content and S/G ratio, and a decrease in carbohydrate content	1067:1177	Diffuse reflectance FT-IR spectroscopy and pyrolysis-GC/MS analyses showed significant alternation in wood chemistry of transgenic plants including an increase in lignin content and S/G ratio, and a decrease in carbohydrate content.
24997793	6	115	theme	transgenic	1067:1076	arg1	increase					1098:1105	an increase	1095:1105	an increase in lignin content and S/G ratio	1095:1137	Diffuse reflectance FT-IR spectroscopy and pyrolysis-GC/MS analyses showed significant alternation in wood chemistry of transgenic plants including an increase in lignin content and S/G ratio, and a decrease in carbohydrate content.
24997793	9	116	theme	increased	1799:1807	arg1	recalcitrance					1809:1821	their increased recalcitrance	1793:1821	their increased recalcitrance caused by the increased lignin content	1793:1860	Enzymatic saccharification without pretreatment resulted in significant decreases of the yields of Gal, Glc, Xyl and Man in transgenic lines, consistent with their increased recalcitrance caused by the increased lignin content.
24997793	2	117	theme	glucuronoyl	412:422	arg1	GCEs					435:438	GCEs	435:438	GCEs	435:438	This linkage is proposed to be hydrolysed by glucuronoyl esterases (GCEs) secreted by wood-degrading fungi.
24997793	2	117	theme	glucuronoyl	412:422	arg1	esterases					424:432	glucuronoyl esterases	412:432	glucuronoyl esterases (GCEs) secreted by wood-degrading fungi	412:472	This linkage is proposed to be hydrolysed by glucuronoyl esterases (GCEs) secreted by wood-degrading fungi.
24997793	5	118	theme	cell	758:761	arg1	wall					763:766	the plant cell wall	748:766	the plant cell wall	748:766	The recombinant enzyme, which was targeted to the plant cell wall using the signal peptide from hybrid aspen cellulase PttCel9B3, was constitutively expressed resulting in the appearance of GCE activity in protein extracts from developing wood.
24997793	1	119	theme	4-O-Me-GlcA	297:307	arg1	moieties					310:317	4-O-methyl-α-D-glucuronic acid (4-O-Me-GlcA) moieties	265:317	4-O-methyl-α-D-glucuronic acid (4-O-Me-GlcA) moieties in glucuronoxylan and alcohol groups in lignin	265:364	The secondary walls of angiosperms contain large amounts of glucuronoxylan that is thought to be covalently linked to lignin via ester bonds between 4-O-methyl-α-D-glucuronic acid (4-O-Me-GlcA) moieties in glucuronoxylan and alcohol groups in lignin.
24997793	7	120	dep	%	1294:1294	arg1	5					1293:1293	5	1293:1293	5	1293:1293	Sequential wood extractions confirmed a massive (+43%) increase of Klason lignin, which was accompanied by a ca. 5% decrease in cellulose, and ca. 20% decrease in wood extractives.
24997793	8	121	theme	4-O-Me-GlcA	1562:1572	arg1	moieties					1574:1581	4-O-Me-GlcA moieties	1562:1581	4-O-Me-GlcA moieties	1562:1581	Analysis of the monosaccharide composition using methanolysis showed a reduction of 4-O-Me-GlcA content without a change in Xyl contents in transgenic lines, suggesting that the covalent links between 4-O-Me-GlcA moieties and lignin protect these moieties from degradation.
24997793	0	122	dep	Expression	0:9	arg1	effects					56:62	effects	56:62	Expression of a fungal glucuronoyl esterase in Populus: effects on wood properties and saccharification efficiency.	0:114	Expression of a fungal glucuronoyl esterase in Populus: effects on wood properties and saccharification efficiency.
24997793	9	123	from	Man	1752:1754	arg1	lines					1770:1774	transgenic lines	1759:1774	transgenic lines	1759:1774	Enzymatic saccharification without pretreatment resulted in significant decreases of the yields of Gal, Glc, Xyl and Man in transgenic lines, consistent with their increased recalcitrance caused by the increased lignin content.
24997793	9	124	theme	lignin	1847:1852	arg1	content					1854:1860	the increased lignin content	1833:1860	the increased lignin content	1833:1860	Enzymatic saccharification without pretreatment resulted in significant decreases of the yields of Gal, Glc, Xyl and Man in transgenic lines, consistent with their increased recalcitrance caused by the increased lignin content.
24997793	3	125	theme	GCE	516:518	arg1	overexpression					496:509	overexpression	496:509	overexpression of a GCE from the white-rot basidiomycete Phanerochaete carnosa, PcGCE, in hybrid aspen	496:597	We report effects of overexpression of a GCE from the white-rot basidiomycete Phanerochaete carnosa, PcGCE, in hybrid aspen (Populus tremula L. x tremuloides Michx.)
24997793	9	126	from	Xyl	1744:1746	arg1	lines					1770:1774	transgenic lines	1759:1774	transgenic lines	1759:1774	Enzymatic saccharification without pretreatment resulted in significant decreases of the yields of Gal, Glc, Xyl and Man in transgenic lines, consistent with their increased recalcitrance caused by the increased lignin content.
24997793	7	127	from	decrease	1296:1303	arg1	cellulose					1308:1316	cellulose	1308:1316	cellulose	1308:1316	Sequential wood extractions confirmed a massive (+43%) increase of Klason lignin, which was accompanied by a ca. 5% decrease in cellulose, and ca. 20% decrease in wood extractives.
24997793	7	128	theme	Klason	1247:1252	arg1	lignin					1254:1259	Klason lignin	1247:1259	Klason lignin	1247:1259	Sequential wood extractions confirmed a massive (+43%) increase of Klason lignin, which was accompanied by a ca. 5% decrease in cellulose, and ca. 20% decrease in wood extractives.
24997793	10	129	theme	acid	1913:1916	arg1	pretreatment					1918:1929	acid pretreatment	1913:1929	acid pretreatment	1913:1929	In contrast, the enzymatic saccharification after acid pretreatment resulted in Glc yields similar to wild-type despite of their lower cellulose content.
24997793	8	130	theme	composition	1392:1402	arg1	Analysis					1361:1368	Analysis	1361:1368	Analysis of the monosaccharide composition using methanolysis	1361:1421	Analysis of the monosaccharide composition using methanolysis showed a reduction of 4-O-Me-GlcA content without a change in Xyl contents in transgenic lines, suggesting that the covalent links between 4-O-Me-GlcA moieties and lignin protect these moieties from degradation.
24997793	7	131	theme	Sequential	1180:1189	arg1	extractions					1196:1206	Sequential wood extractions	1180:1206	Sequential wood extractions	1180:1206	Sequential wood extractions confirmed a massive (+43%) increase of Klason lignin, which was accompanied by a ca. 5% decrease in cellulose, and ca. 20% decrease in wood extractives.
28963723	5	0	from	WF	998:999	arg1	amylose					956:962	amylose	956:962	amylose	956:962	Furthermore, the dominance of G' than G″ over the range of LVE and high fracture stress at high level of Hylon VII confirmed the high gel strength which can be attributed to the retrogradation of amylose and reduction of amylopectin from WF.
28963723	5	0	from	WF	998:999	arg1	retrogradation					938:951	the retrogradation	934:951	the retrogradation of amylose and reduction of amylopectin from WF	934:999	Furthermore, the dominance of G' than G″ over the range of LVE and high fracture stress at high level of Hylon VII confirmed the high gel strength which can be attributed to the retrogradation of amylose and reduction of amylopectin from WF.
28963723	5	0	from	WF	998:999	arg1	reduction					968:976	reduction	968:976	reduction	968:976	Furthermore, the dominance of G' than G″ over the range of LVE and high fracture stress at high level of Hylon VII confirmed the high gel strength which can be attributed to the retrogradation of amylose and reduction of amylopectin from WF.
28963723	9	1	theme	PRACTICAL	1441:1449	arg1	properties					1492:1501	PRACTICAL APPLICATIONS Small and large deformation properties	1441:1501	PRACTICAL APPLICATIONS Small and large deformation properties	1441:1501	PRACTICAL APPLICATIONS Small and large deformation properties can provide profound insights toward the gel structure.
28963723	7	2	theme	gel	1193:1195	arg1	structures					1205:1214	the gel network structures	1189:1214	the gel network structures	1189:1214	High value of α revealed a lower number of interactions within the gel network structures.
28963723	6	3	theme	frequency	1011:1019	arg1	dependency					1021:1030	The less frequency dependency	1002:1030	The less frequency dependency of BCG	1002:1037	The less frequency dependency of BCG revealed the solid-like response and strong gels structures with more elastic network.
28963723	8	4	theme	textural	1420:1427	arg1	parameters					1429:1438	the special textural parameters	1408:1438	the special textural parameters	1408:1438	Consequently, due to the high gel strength of BCG of WF/H, it can be exploited at high thermal operations such as retort processing as well as it can be utilized for dysphagia therapy due to the special textural parameters.
28963723	13	5	theme	current	2080:2086	arg1	work					2088:2091	the current work	2076:2091	the current work	2076:2091	Therefore, the current work was accomplished to provide some knowledge about the binary composite gel of WF and Hylon VII starch such as the gel strength, fracture stress, fracture strain and material stiffness which enables us to evaluate the nature of starch gels for further applications such as drug delivery systems, elderly diet and further processes.
28963723	6	6	theme	more	1104:1107	arg1	network					1117:1123	more elastic network	1104:1123	more elastic network	1104:1123	The less frequency dependency of BCG revealed the solid-like response and strong gels structures with more elastic network.
28963723	9	7	theme	Small	1464:1468	arg1	properties					1492:1501	PRACTICAL APPLICATIONS Small and large deformation properties	1441:1501	PRACTICAL APPLICATIONS Small and large deformation properties	1441:1501	PRACTICAL APPLICATIONS Small and large deformation properties can provide profound insights toward the gel structure.
28963723	3	8	theme	VII	590:592	arg1	addition					594:601	Hylon VII addition	584:601	Hylon VII addition	584:601	However, the gel firmness was increased by Hylon VII addition, but the springiness was reduced.
28963723	5	9	from	retrogradation	938:951	arg1	WF					998:999	WF	998:999	WF	998:999	Furthermore, the dominance of G' than G″ over the range of LVE and high fracture stress at high level of Hylon VII confirmed the high gel strength which can be attributed to the retrogradation of amylose and reduction of amylopectin from WF.
28963723	1	10	theme	Hylon	150:154	arg1	VII					156:158	Hylon VII	150:158	Hylon VII	150:158	The effect of addition of high amylose corn starch (Hylon VII or H) to wheat flour (WF) on the mechanical properties of the resulting binary composite gels (BCG) under small and large deformations was evaluated.
28963723	2	11	theme	profile	503:509	arg1	analysis					511:518	a texture profile analysis	493:518	a texture profile analysis in compression mode	493:538	To this end, the composite gels at different ratios of WF/H including 95:5, 90:10, and 85:15 were tested under a linear viscoelastic regime (LVE) in oscillatory angular frequency and a texture profile analysis in compression mode.
28963723	6	12	theme	strong	1076:1081	arg1	gels					1083:1086	strong gels	1076:1086	strong gels	1076:1086	The less frequency dependency of BCG revealed the solid-like response and strong gels structures with more elastic network.
28963723	11	13	theme	common	1823:1828	arg1	disorders					1791:1799	chewing and swallowing disorders	1768:1799	chewing and swallowing disorders such as dysphagia	1768:1817	Since, achieving the desired texture of foods has a chief impact on the target consumers, and chewing and swallowing disorders such as dysphagia are common problems in older people, more effort is needed to modify the texture of food with a soft structure.
28963723	11	13	theme	common	1823:1828	arg1	dysphagia					1809:1817	dysphagia	1809:1817	dysphagia	1809:1817	Since, achieving the desired texture of foods has a chief impact on the target consumers, and chewing and swallowing disorders such as dysphagia are common problems in older people, more effort is needed to modify the texture of food with a soft structure.
28963723	11	13	theme	common	1823:1828	arg1	problems					1830:1837	common problems	1823:1837	common problems in older people	1823:1853	Since, achieving the desired texture of foods has a chief impact on the target consumers, and chewing and swallowing disorders such as dysphagia are common problems in older people, more effort is needed to modify the texture of food with a soft structure.
28963723	13	14	theme	fracture	2237:2244	arg1	strain					2246:2251	fracture strain	2237:2251	fracture strain	2237:2251	Therefore, the current work was accomplished to provide some knowledge about the binary composite gel of WF and Hylon VII starch such as the gel strength, fracture stress, fracture strain and material stiffness which enables us to evaluate the nature of starch gels for further applications such as drug delivery systems, elderly diet and further processes.
28963723	11	15	theme	older	1842:1846	arg1	people					1848:1853	older people	1842:1853	older people	1842:1853	Since, achieving the desired texture of foods has a chief impact on the target consumers, and chewing and swallowing disorders such as dysphagia are common problems in older people, more effort is needed to modify the texture of food with a soft structure.
28963723	5	16	from	level	856:860	arg1	dominance					777:785	the dominance	773:785	the dominance of G' than G″ over the range of LVE and high fracture stress at high level of Hylon VII	773:873	Furthermore, the dominance of G' than G″ over the range of LVE and high fracture stress at high level of Hylon VII confirmed the high gel strength which can be attributed to the retrogradation of amylose and reduction of amylopectin from WF.
28963723	2	17	theme	viscoelastic	430:441	arg1	regime					443:448	a linear viscoelastic regime	421:448	a linear viscoelastic regime (LVE)	421:454	To this end, the composite gels at different ratios of WF/H including 95:5, 90:10, and 85:15 were tested under a linear viscoelastic regime (LVE) in oscillatory angular frequency and a texture profile analysis in compression mode.
28963723	2	17	theme	viscoelastic	430:441	arg1	LVE					451:453	LVE	451:453	LVE	451:453	To this end, the composite gels at different ratios of WF/H including 95:5, 90:10, and 85:15 were tested under a linear viscoelastic regime (LVE) in oscillatory angular frequency and a texture profile analysis in compression mode.
28963723	13	18	theme	gel	2206:2208	arg1	strength					2210:2217	gel strength	2206:2217	gel strength	2206:2217	Therefore, the current work was accomplished to provide some knowledge about the binary composite gel of WF and Hylon VII starch such as the gel strength, fracture stress, fracture strain and material stiffness which enables us to evaluate the nature of starch gels for further applications such as drug delivery systems, elderly diet and further processes.
28963723	11	19	theme	target	1746:1751	arg1	consumers					1753:1761	the target consumers	1742:1761	the target consumers	1742:1761	Since, achieving the desired texture of foods has a chief impact on the target consumers, and chewing and swallowing disorders such as dysphagia are common problems in older people, more effort is needed to modify the texture of food with a soft structure.
28963723	1	20	theme	wheat	169:173	arg1	WF					182:183	WF	182:183	WF	182:183	The effect of addition of high amylose corn starch (Hylon VII or H) to wheat flour (WF) on the mechanical properties of the resulting binary composite gels (BCG) under small and large deformations was evaluated.
28963723	1	20	theme	wheat	169:173	arg1	flour					175:179	wheat flour	169:179	wheat flour (WF)	169:184	The effect of addition of high amylose corn starch (Hylon VII or H) to wheat flour (WF) on the mechanical properties of the resulting binary composite gels (BCG) under small and large deformations was evaluated.
28963723	2	21	theme	oscillatory	459:469	arg1	frequency					479:487	oscillatory angular frequency	459:487	oscillatory angular frequency	459:487	To this end, the composite gels at different ratios of WF/H including 95:5, 90:10, and 85:15 were tested under a linear viscoelastic regime (LVE) in oscillatory angular frequency and a texture profile analysis in compression mode.
28963723	6	22	theme	solid-like	1052:1061	arg1	response					1063:1070	the solid-like response	1048:1070	the solid-like response	1048:1070	The less frequency dependency of BCG revealed the solid-like response and strong gels structures with more elastic network.
28963723	13	23	theme	Hylon	2177:2181	arg1	starch					2187:2192	WF and Hylon VII starch	2170:2192	starch	2187:2192	Therefore, the current work was accomplished to provide some knowledge about the binary composite gel of WF and Hylon VII starch such as the gel strength, fracture stress, fracture strain and material stiffness which enables us to evaluate the nature of starch gels for further applications such as drug delivery systems, elderly diet and further processes.
28963723	13	24	theme	starch	2187:2192	arg1	gel					2163:2165	the binary composite gel	2142:2165	the binary composite gel of WF and Hylon VII starch such as the gel strength, fracture stress, fracture strain and material stiffness which enables us to evaluate the nature of starch gels for further applications such as drug delivery systems, elderly diet and further processes	2142:2420	Therefore, the current work was accomplished to provide some knowledge about the binary composite gel of WF and Hylon VII starch such as the gel strength, fracture stress, fracture strain and material stiffness which enables us to evaluate the nature of starch gels for further applications such as drug delivery systems, elderly diet and further processes.
28963723	5	25	theme	high	889:892	arg1	strength					898:905	the high gel strength	885:905	the high gel strength which can be attributed to the retrogradation of amylose and reduction of amylopectin from WF	885:999	Furthermore, the dominance of G' than G″ over the range of LVE and high fracture stress at high level of Hylon VII confirmed the high gel strength which can be attributed to the retrogradation of amylose and reduction of amylopectin from WF.
28963723	10	26	theme	textural	1633:1640	arg1	properties					1642:1651	the textural properties	1629:1651	the textural properties of the gel matrices	1629:1671	The former gets knowledge about dynamic rheology and the latter gives the textural properties of the gel matrices.
28963723	0	27	theme	flour	62:66	arg1	gels					48:51	binary composite gels	31:51	binary composite gels of wheat flour and high amylose corn starch	31:95	Rheological characteristics of binary composite gels of wheat flour and high amylose corn starch.
28963723	1	28	theme	amylose	129:135	arg1	starch					142:147	high amylose corn starch	124:147	high amylose corn starch (Hylon VII or H)	124:164	The effect of addition of high amylose corn starch (Hylon VII or H) to wheat flour (WF) on the mechanical properties of the resulting binary composite gels (BCG) under small and large deformations was evaluated.
28963723	12	29	theme	thermal	2015:2021	arg1	processing					2023:2032	high thermal processing	2010:2032	high thermal processing	2010:2032	On the contrary, supplying a more gel strength network, which can withstand at high thermal processing and not collapse, is so vital.
28963723	11	30	from	problems	1830:1837	arg1	people					1848:1853	older people	1842:1853	older people	1842:1853	Since, achieving the desired texture of foods has a chief impact on the target consumers, and chewing and swallowing disorders such as dysphagia are common problems in older people, more effort is needed to modify the texture of food with a soft structure.
28963723	0	31	theme	high	72:75	arg1	starch					90:95	high amylose corn starch	72:95	high amylose corn starch	72:95	Rheological characteristics of binary composite gels of wheat flour and high amylose corn starch.
28963723	1	32	theme	small	266:270	arg1	deformations					282:293	small and large deformations	266:293	small and large deformations	266:293	The effect of addition of high amylose corn starch (Hylon VII or H) to wheat flour (WF) on the mechanical properties of the resulting binary composite gels (BCG) under small and large deformations was evaluated.
28963723	1	33	theme	starch	142:147	arg1	addition					112:119	addition	112:119	addition of high amylose corn starch (Hylon VII or H) to wheat flour (WF)	112:184	The effect of addition of high amylose corn starch (Hylon VII or H) to wheat flour (WF) on the mechanical properties of the resulting binary composite gels (BCG) under small and large deformations was evaluated.
28963723	8	34	theme	high	1299:1302	arg1	processing					1338:1347	retort processing	1331:1347	retort processing	1331:1347	Consequently, due to the high gel strength of BCG of WF/H, it can be exploited at high thermal operations such as retort processing as well as it can be utilized for dysphagia therapy due to the special textural parameters.
28963723	8	34	theme	high	1299:1302	arg1	operations					1312:1321	high thermal operations	1299:1321	high thermal operations such as retort processing	1299:1347	Consequently, due to the high gel strength of BCG of WF/H, it can be exploited at high thermal operations such as retort processing as well as it can be utilized for dysphagia therapy due to the special textural parameters.
28963723	0	35	theme	corn	85:88	arg1	starch					90:95	high amylose corn starch	72:95	high amylose corn starch	72:95	Rheological characteristics of binary composite gels of wheat flour and high amylose corn starch.
28963723	1	36	theme	large	276:280	arg1	deformations					282:293	small and large deformations	266:293	small and large deformations	266:293	The effect of addition of high amylose corn starch (Hylon VII or H) to wheat flour (WF) on the mechanical properties of the resulting binary composite gels (BCG) under small and large deformations was evaluated.
28963723	5	37	theme	LVE	819:821	arg1	stress					841:846	LVE and high fracture stress	819:846	LVE and high fracture stress	819:846	Furthermore, the dominance of G' than G″ over the range of LVE and high fracture stress at high level of Hylon VII confirmed the high gel strength which can be attributed to the retrogradation of amylose and reduction of amylopectin from WF.
28963723	2	38	theme	compression	523:533	arg1	mode					535:538	compression mode	523:538	compression mode	523:538	To this end, the composite gels at different ratios of WF/H including 95:5, 90:10, and 85:15 were tested under a linear viscoelastic regime (LVE) in oscillatory angular frequency and a texture profile analysis in compression mode.
28963723	13	39	theme	gels	2326:2329	arg1	nature					2309:2314	the nature	2305:2314	the nature of starch gels for further applications such as drug delivery systems, elderly diet and further processes	2305:2420	Therefore, the current work was accomplished to provide some knowledge about the binary composite gel of WF and Hylon VII starch such as the gel strength, fracture stress, fracture strain and material stiffness which enables us to evaluate the nature of starch gels for further applications such as drug delivery systems, elderly diet and further processes.
28963723	13	40	theme	material	2257:2264	arg1	stiffness					2266:2274	material stiffness	2257:2274	material stiffness	2257:2274	Therefore, the current work was accomplished to provide some knowledge about the binary composite gel of WF and Hylon VII starch such as the gel strength, fracture stress, fracture strain and material stiffness which enables us to evaluate the nature of starch gels for further applications such as drug delivery systems, elderly diet and further processes.
28963723	5	41	theme	high	827:830	arg1	stress					841:846	LVE and high fracture stress	819:846	LVE and high fracture stress	819:846	Furthermore, the dominance of G' than G″ over the range of LVE and high fracture stress at high level of Hylon VII confirmed the high gel strength which can be attributed to the retrogradation of amylose and reduction of amylopectin from WF.
28963723	0	42	theme	Rheological	0:10	arg1	characteristics					12:26	Rheological characteristics	0:26	Rheological characteristics of binary composite gels of wheat flour and high amylose corn starch.	0:96	Rheological characteristics of binary composite gels of wheat flour and high amylose corn starch.
28963723	1	43	from	effect	102:107	arg1	properties					204:213	the mechanical properties	189:213	the mechanical properties of the resulting binary composite gels (BCG) under small and large deformations	189:293	The effect of addition of high amylose corn starch (Hylon VII or H) to wheat flour (WF) on the mechanical properties of the resulting binary composite gels (BCG) under small and large deformations was evaluated.
28963723	11	44	theme	food	1903:1906	arg1	texture					1892:1898	the texture	1888:1898	the texture of food with a soft structure	1888:1928	Since, achieving the desired texture of foods has a chief impact on the target consumers, and chewing and swallowing disorders such as dysphagia are common problems in older people, more effort is needed to modify the texture of food with a soft structure.
28963723	2	45	theme	different	345:353	arg1	ratios					355:360	different ratios	345:360	different ratios of WF/H	345:368	To this end, the composite gels at different ratios of WF/H including 95:5, 90:10, and 85:15 were tested under a linear viscoelastic regime (LVE) in oscillatory angular frequency and a texture profile analysis in compression mode.
28963723	12	46	theme	gel	1965:1967	arg1	network					1978:1984	a more gel strength network	1958:1984	a more gel strength network	1958:1984	On the contrary, supplying a more gel strength network, which can withstand at high thermal processing and not collapse, is so vital.
28963723	1	47	theme	resulting	222:230	arg1	gels					249:252	the resulting binary composite gels	218:252	the resulting binary composite gels (BCG) under small and large deformations	218:293	The effect of addition of high amylose corn starch (Hylon VII or H) to wheat flour (WF) on the mechanical properties of the resulting binary composite gels (BCG) under small and large deformations was evaluated.
28963723	1	47	theme	resulting	222:230	arg1	BCG					255:257	BCG	255:257	BCG	255:257	The effect of addition of high amylose corn starch (Hylon VII or H) to wheat flour (WF) on the mechanical properties of the resulting binary composite gels (BCG) under small and large deformations was evaluated.
28963723	13	48	theme	binary	2146:2151	arg1	gel					2163:2165	the binary composite gel	2142:2165	the binary composite gel of WF and Hylon VII starch such as the gel strength, fracture stress, fracture strain and material stiffness which enables us to evaluate the nature of starch gels for further applications such as drug delivery systems, elderly diet and further processes	2142:2420	Therefore, the current work was accomplished to provide some knowledge about the binary composite gel of WF and Hylon VII starch such as the gel strength, fracture stress, fracture strain and material stiffness which enables us to evaluate the nature of starch gels for further applications such as drug delivery systems, elderly diet and further processes.
28963723	7	49	theme	α	1140:1140	arg1	value					1131:1135	High value	1126:1135	High value of α	1126:1140	High value of α revealed a lower number of interactions within the gel network structures.
28963723	10	50	theme	gel	1660:1662	arg1	matrices					1664:1671	the gel matrices	1656:1671	the gel matrices	1656:1671	The former gets knowledge about dynamic rheology and the latter gives the textural properties of the gel matrices.
28963723	11	51	theme	foods	1714:1718	arg1	texture					1703:1709	the desired texture	1691:1709	the desired texture of foods	1691:1718	Since, achieving the desired texture of foods has a chief impact on the target consumers, and chewing and swallowing disorders such as dysphagia are common problems in older people, more effort is needed to modify the texture of food with a soft structure.
28963723	0	52	theme	composite	38:46	arg1	gels					48:51	binary composite gels	31:51	binary composite gels of wheat flour and high amylose corn starch	31:95	Rheological characteristics of binary composite gels of wheat flour and high amylose corn starch.
28963723	1	53	theme	composite	239:247	arg1	gels					249:252	the resulting binary composite gels	218:252	the resulting binary composite gels (BCG) under small and large deformations	218:293	The effect of addition of high amylose corn starch (Hylon VII or H) to wheat flour (WF) on the mechanical properties of the resulting binary composite gels (BCG) under small and large deformations was evaluated.
28963723	1	53	theme	composite	239:247	arg1	BCG					255:257	BCG	255:257	BCG	255:257	The effect of addition of high amylose corn starch (Hylon VII or H) to wheat flour (WF) on the mechanical properties of the resulting binary composite gels (BCG) under small and large deformations was evaluated.
28963723	13	54	dep	strength	2210:2217	arg1	the					2202:2204	the	2202:2204	the	2202:2204	Therefore, the current work was accomplished to provide some knowledge about the binary composite gel of WF and Hylon VII starch such as the gel strength, fracture stress, fracture strain and material stiffness which enables us to evaluate the nature of starch gels for further applications such as drug delivery systems, elderly diet and further processes.
28963723	13	55	theme	further	2335:2341	arg1	diet					2395:2398	elderly diet	2387:2398	elderly diet	2387:2398	Therefore, the current work was accomplished to provide some knowledge about the binary composite gel of WF and Hylon VII starch such as the gel strength, fracture stress, fracture strain and material stiffness which enables us to evaluate the nature of starch gels for further applications such as drug delivery systems, elderly diet and further processes.
28963723	13	55	theme	further	2335:2341	arg1	systems					2378:2384	drug delivery systems	2364:2384	drug delivery systems	2364:2384	Therefore, the current work was accomplished to provide some knowledge about the binary composite gel of WF and Hylon VII starch such as the gel strength, fracture stress, fracture strain and material stiffness which enables us to evaluate the nature of starch gels for further applications such as drug delivery systems, elderly diet and further processes.
28963723	13	55	theme	further	2335:2341	arg1	applications					2343:2354	further applications	2335:2354	further applications such as drug delivery systems, elderly diet and further processes	2335:2420	Therefore, the current work was accomplished to provide some knowledge about the binary composite gel of WF and Hylon VII starch such as the gel strength, fracture stress, fracture strain and material stiffness which enables us to evaluate the nature of starch gels for further applications such as drug delivery systems, elderly diet and further processes.
28963723	13	55	theme	further	2335:2341	arg1	processes					2412:2420	further processes	2404:2420	further processes	2404:2420	Therefore, the current work was accomplished to provide some knowledge about the binary composite gel of WF and Hylon VII starch such as the gel strength, fracture stress, fracture strain and material stiffness which enables us to evaluate the nature of starch gels for further applications such as drug delivery systems, elderly diet and further processes.
28963723	5	56	theme	VII	871:873	arg1	level					856:860	high level	851:860	high level of Hylon VII	851:873	Furthermore, the dominance of G' than G″ over the range of LVE and high fracture stress at high level of Hylon VII confirmed the high gel strength which can be attributed to the retrogradation of amylose and reduction of amylopectin from WF.
28963723	9	57	theme	APPLICATIONS	1451:1462	arg1	properties					1492:1501	PRACTICAL APPLICATIONS Small and large deformation properties	1441:1501	PRACTICAL APPLICATIONS Small and large deformation properties	1441:1501	PRACTICAL APPLICATIONS Small and large deformation properties can provide profound insights toward the gel structure.
28963723	2	58	from	analysis	511:518	arg1	mode					535:538	compression mode	523:538	compression mode	523:538	To this end, the composite gels at different ratios of WF/H including 95:5, 90:10, and 85:15 were tested under a linear viscoelastic regime (LVE) in oscillatory angular frequency and a texture profile analysis in compression mode.
28963723	8	59	used	utilized	1370:1377	arg2	it					1360:1361	it	1360:1361	it	1360:1361	Consequently, due to the high gel strength of BCG of WF/H, it can be exploited at high thermal operations such as retort processing as well as it can be utilized for dysphagia therapy due to the special textural parameters.
28963723	8	60	theme	high	1242:1245	arg1	strength					1251:1258	the high gel strength	1238:1258	the high gel strength of BCG of WF/H	1238:1273	Consequently, due to the high gel strength of BCG of WF/H, it can be exploited at high thermal operations such as retort processing as well as it can be utilized for dysphagia therapy due to the special textural parameters.
28963723	11	61	theme	swallowing	1780:1789	arg1	disorders					1791:1799	chewing and swallowing disorders	1768:1799	chewing and swallowing disorders such as dysphagia	1768:1817	Since, achieving the desired texture of foods has a chief impact on the target consumers, and chewing and swallowing disorders such as dysphagia are common problems in older people, more effort is needed to modify the texture of food with a soft structure.
28963723	11	61	theme	swallowing	1780:1789	arg1	dysphagia					1809:1817	dysphagia	1809:1817	dysphagia	1809:1817	Since, achieving the desired texture of foods has a chief impact on the target consumers, and chewing and swallowing disorders such as dysphagia are common problems in older people, more effort is needed to modify the texture of food with a soft structure.
28963723	11	61	theme	swallowing	1780:1789	arg1	problems					1830:1837	common problems	1823:1837	common problems in older people	1823:1853	Since, achieving the desired texture of foods has a chief impact on the target consumers, and chewing and swallowing disorders such as dysphagia are common problems in older people, more effort is needed to modify the texture of food with a soft structure.
28963723	5	62	theme	stress	841:846	arg1	range					810:814	the range	806:814	the range of LVE and high fracture stress	806:846	Furthermore, the dominance of G' than G″ over the range of LVE and high fracture stress at high level of Hylon VII confirmed the high gel strength which can be attributed to the retrogradation of amylose and reduction of amylopectin from WF.
28963723	5	63	theme	high	851:854	arg1	level					856:860	high level	851:860	high level of Hylon VII	851:873	Furthermore, the dominance of G' than G″ over the range of LVE and high fracture stress at high level of Hylon VII confirmed the high gel strength which can be attributed to the retrogradation of amylose and reduction of amylopectin from WF.
28963723	5	64	theme	amylopectin	981:991	arg1	amylose					956:962	amylose	956:962	amylose	956:962	Furthermore, the dominance of G' than G″ over the range of LVE and high fracture stress at high level of Hylon VII confirmed the high gel strength which can be attributed to the retrogradation of amylose and reduction of amylopectin from WF.
28963723	5	64	theme	amylopectin	981:991	arg1	reduction					968:976	reduction	968:976	reduction	968:976	Furthermore, the dominance of G' than G″ over the range of LVE and high fracture stress at high level of Hylon VII confirmed the high gel strength which can be attributed to the retrogradation of amylose and reduction of amylopectin from WF.
28963723	3	65	theme	Hylon	584:588	arg1	addition					594:601	Hylon VII addition	584:601	Hylon VII addition	584:601	However, the gel firmness was increased by Hylon VII addition, but the springiness was reduced.
28963723	9	66	theme	large	1474:1478	arg1	properties					1492:1501	PRACTICAL APPLICATIONS Small and large deformation properties	1441:1501	PRACTICAL APPLICATIONS Small and large deformation properties	1441:1501	PRACTICAL APPLICATIONS Small and large deformation properties can provide profound insights toward the gel structure.
28963723	9	67	theme	profound	1515:1522	arg1	insights					1524:1531	profound insights	1515:1531	profound insights toward the gel structure	1515:1556	PRACTICAL APPLICATIONS Small and large deformation properties can provide profound insights toward the gel structure.
28963723	6	68	theme	elastic	1109:1115	arg1	network					1117:1123	more elastic network	1104:1123	more elastic network	1104:1123	The less frequency dependency of BCG revealed the solid-like response and strong gels structures with more elastic network.
28963723	1	69	theme	mechanical	193:202	arg1	properties					204:213	the mechanical properties	189:213	the mechanical properties of the resulting binary composite gels (BCG) under small and large deformations	189:293	The effect of addition of high amylose corn starch (Hylon VII or H) to wheat flour (WF) on the mechanical properties of the resulting binary composite gels (BCG) under small and large deformations was evaluated.
28963723	2	70	theme	angular	471:477	arg1	frequency					479:487	oscillatory angular frequency	459:487	oscillatory angular frequency	459:487	To this end, the composite gels at different ratios of WF/H including 95:5, 90:10, and 85:15 were tested under a linear viscoelastic regime (LVE) in oscillatory angular frequency and a texture profile analysis in compression mode.
28963723	6	71	theme	less	1006:1009	arg1	dependency					1021:1030	The less frequency dependency	1002:1030	The less frequency dependency of BCG	1002:1037	The less frequency dependency of BCG revealed the solid-like response and strong gels structures with more elastic network.
28963723	8	72	theme	special	1412:1418	arg1	parameters					1429:1438	the special textural parameters	1408:1438	the special textural parameters	1408:1438	Consequently, due to the high gel strength of BCG of WF/H, it can be exploited at high thermal operations such as retort processing as well as it can be utilized for dysphagia therapy due to the special textural parameters.
28963723	13	73	theme	elderly	2387:2393	arg1	diet					2395:2398	elderly diet	2387:2398	elderly diet	2387:2398	Therefore, the current work was accomplished to provide some knowledge about the binary composite gel of WF and Hylon VII starch such as the gel strength, fracture stress, fracture strain and material stiffness which enables us to evaluate the nature of starch gels for further applications such as drug delivery systems, elderly diet and further processes.
28963723	4	74	theme	dilution	714:721	arg1	effect					723:728	no dilution effect	711:728	no dilution effect	711:728	Since the adhesiveness and cohesiveness were not significantly different, no dilution effect was observed for the samples.
28963723	13	75	theme	fracture	2220:2227	arg1	stress					2229:2234	fracture stress	2220:2234	fracture stress	2220:2234	Therefore, the current work was accomplished to provide some knowledge about the binary composite gel of WF and Hylon VII starch such as the gel strength, fracture stress, fracture strain and material stiffness which enables us to evaluate the nature of starch gels for further applications such as drug delivery systems, elderly diet and further processes.
28963723	10	76	theme	dynamic	1591:1597	arg1	rheology					1599:1606	dynamic rheology	1591:1606	dynamic rheology	1591:1606	The former gets knowledge about dynamic rheology and the latter gives the textural properties of the gel matrices.
28963723	2	77	theme	texture	495:501	arg1	analysis					511:518	a texture profile analysis	493:518	a texture profile analysis in compression mode	493:538	To this end, the composite gels at different ratios of WF/H including 95:5, 90:10, and 85:15 were tested under a linear viscoelastic regime (LVE) in oscillatory angular frequency and a texture profile analysis in compression mode.
28963723	11	78	from	impact	1732:1737	arg1	consumers					1753:1761	the target consumers	1742:1761	the target consumers	1742:1761	Since, achieving the desired texture of foods has a chief impact on the target consumers, and chewing and swallowing disorders such as dysphagia are common problems in older people, more effort is needed to modify the texture of food with a soft structure.
28963723	13	79	theme	drug	2364:2367	arg1	systems					2378:2384	drug delivery systems	2364:2384	drug delivery systems	2364:2384	Therefore, the current work was accomplished to provide some knowledge about the binary composite gel of WF and Hylon VII starch such as the gel strength, fracture stress, fracture strain and material stiffness which enables us to evaluate the nature of starch gels for further applications such as drug delivery systems, elderly diet and further processes.
28963723	8	80	theme	BCG	1263:1265	arg1	strength					1251:1258	the high gel strength	1238:1258	the high gel strength of BCG of WF/H	1238:1273	Consequently, due to the high gel strength of BCG of WF/H, it can be exploited at high thermal operations such as retort processing as well as it can be utilized for dysphagia therapy due to the special textural parameters.
28963723	9	81	theme	deformation	1480:1490	arg1	properties					1492:1501	PRACTICAL APPLICATIONS Small and large deformation properties	1441:1501	PRACTICAL APPLICATIONS Small and large deformation properties	1441:1501	PRACTICAL APPLICATIONS Small and large deformation properties can provide profound insights toward the gel structure.
28963723	2	82	theme	linear	423:428	arg1	regime					443:448	a linear viscoelastic regime	421:448	a linear viscoelastic regime (LVE)	421:454	To this end, the composite gels at different ratios of WF/H including 95:5, 90:10, and 85:15 were tested under a linear viscoelastic regime (LVE) in oscillatory angular frequency and a texture profile analysis in compression mode.
28963723	2	82	theme	linear	423:428	arg1	LVE					451:453	LVE	451:453	LVE	451:453	To this end, the composite gels at different ratios of WF/H including 95:5, 90:10, and 85:15 were tested under a linear viscoelastic regime (LVE) in oscillatory angular frequency and a texture profile analysis in compression mode.
28963723	8	83	theme	WF/H	1270:1273	arg1	BCG					1263:1265	BCG	1263:1265	BCG of WF/H	1263:1273	Consequently, due to the high gel strength of BCG of WF/H, it can be exploited at high thermal operations such as retort processing as well as it can be utilized for dysphagia therapy due to the special textural parameters.
28963723	2	84	from	ratios	355:360	arg1	90:10					386:390	90:10	386:390	90:10	386:390	To this end, the composite gels at different ratios of WF/H including 95:5, 90:10, and 85:15 were tested under a linear viscoelastic regime (LVE) in oscillatory angular frequency and a texture profile analysis in compression mode.
28963723	2	84	from	ratios	355:360	arg1	85:15					397:401	85:15	397:401	85:15	397:401	To this end, the composite gels at different ratios of WF/H including 95:5, 90:10, and 85:15 were tested under a linear viscoelastic regime (LVE) in oscillatory angular frequency and a texture profile analysis in compression mode.
28963723	2	84	from	ratios	355:360	arg1	95:5					380:383	95:5	380:383	95:5	380:383	To this end, the composite gels at different ratios of WF/H including 95:5, 90:10, and 85:15 were tested under a linear viscoelastic regime (LVE) in oscillatory angular frequency and a texture profile analysis in compression mode.
28963723	2	84	from	ratios	355:360	arg1	gels					337:340	the composite gels	323:340	the composite gels at different ratios of WF/H including 95:5, 90:10, and 85:15	323:401	To this end, the composite gels at different ratios of WF/H including 95:5, 90:10, and 85:15 were tested under a linear viscoelastic regime (LVE) in oscillatory angular frequency and a texture profile analysis in compression mode.
28963723	7	85	theme	network	1197:1203	arg1	structures					1205:1214	the gel network structures	1189:1214	the gel network structures	1189:1214	High value of α revealed a lower number of interactions within the gel network structures.
28963723	13	86	theme	VII	2183:2185	arg1	starch					2187:2192	WF and Hylon VII starch	2170:2192	starch	2187:2192	Therefore, the current work was accomplished to provide some knowledge about the binary composite gel of WF and Hylon VII starch such as the gel strength, fracture stress, fracture strain and material stiffness which enables us to evaluate the nature of starch gels for further applications such as drug delivery systems, elderly diet and further processes.
28963723	1	87	theme	addition	112:119	arg1	effect					102:107	The effect	98:107	The effect of addition of high amylose corn starch (Hylon VII or H) to wheat flour (WF) on the mechanical properties of the resulting binary composite gels (BCG) under small and large deformations	98:293	The effect of addition of high amylose corn starch (Hylon VII or H) to wheat flour (WF) on the mechanical properties of the resulting binary composite gels (BCG) under small and large deformations was evaluated.
28963723	9	88	theme	gel	1544:1546	arg1	structure					1548:1556	the gel structure	1540:1556	the gel structure	1540:1556	PRACTICAL APPLICATIONS Small and large deformation properties can provide profound insights toward the gel structure.
28963723	6	89	theme	BCG	1035:1037	arg1	dependency					1021:1030	The less frequency dependency	1002:1030	The less frequency dependency of BCG	1002:1037	The less frequency dependency of BCG revealed the solid-like response and strong gels structures with more elastic network.
28963723	5	90	theme	gel	894:896	arg1	strength					898:905	the high gel strength	885:905	the high gel strength which can be attributed to the retrogradation of amylose and reduction of amylopectin from WF	885:999	Furthermore, the dominance of G' than G″ over the range of LVE and high fracture stress at high level of Hylon VII confirmed the high gel strength which can be attributed to the retrogradation of amylose and reduction of amylopectin from WF.
28963723	1	91	theme	high	124:127	arg1	starch					142:147	high amylose corn starch	124:147	high amylose corn starch (Hylon VII or H)	124:164	The effect of addition of high amylose corn starch (Hylon VII or H) to wheat flour (WF) on the mechanical properties of the resulting binary composite gels (BCG) under small and large deformations was evaluated.
28963723	11	92	theme	chief	1726:1730	arg1	impact					1732:1737	a chief impact	1724:1737	a chief impact on the target consumers	1724:1761	Since, achieving the desired texture of foods has a chief impact on the target consumers, and chewing and swallowing disorders such as dysphagia are common problems in older people, more effort is needed to modify the texture of food with a soft structure.
28963723	7	93	theme	interactions	1169:1180	arg1	number					1159:1164	a lower number	1151:1164	a lower number of interactions within the gel network structures	1151:1214	High value of α revealed a lower number of interactions within the gel network structures.
28963723	0	94	theme	amylose	77:83	arg1	starch					90:95	high amylose corn starch	72:95	high amylose corn starch	72:95	Rheological characteristics of binary composite gels of wheat flour and high amylose corn starch.
28963723	5	95	theme	amylose	956:962	arg1	retrogradation					938:951	the retrogradation	934:951	the retrogradation of amylose and reduction of amylopectin from WF	934:999	Furthermore, the dominance of G' than G″ over the range of LVE and high fracture stress at high level of Hylon VII confirmed the high gel strength which can be attributed to the retrogradation of amylose and reduction of amylopectin from WF.
28963723	1	96	theme	corn	137:140	arg1	starch					142:147	high amylose corn starch	124:147	high amylose corn starch (Hylon VII or H)	124:164	The effect of addition of high amylose corn starch (Hylon VII or H) to wheat flour (WF) on the mechanical properties of the resulting binary composite gels (BCG) under small and large deformations was evaluated.
28963723	8	97	theme	thermal	1304:1310	arg1	processing					1338:1347	retort processing	1331:1347	retort processing	1331:1347	Consequently, due to the high gel strength of BCG of WF/H, it can be exploited at high thermal operations such as retort processing as well as it can be utilized for dysphagia therapy due to the special textural parameters.
28963723	8	97	theme	thermal	1304:1310	arg1	operations					1312:1321	high thermal operations	1299:1321	high thermal operations such as retort processing	1299:1347	Consequently, due to the high gel strength of BCG of WF/H, it can be exploited at high thermal operations such as retort processing as well as it can be utilized for dysphagia therapy due to the special textural parameters.
28963723	6	98	dep	response	1063:1070	arg1	structures					1088:1097	structures	1088:1097	structures	1088:1097	The less frequency dependency of BCG revealed the solid-like response and strong gels structures with more elastic network.
28963723	0	99	theme	starch	90:95	arg1	gels					48:51	binary composite gels	31:51	binary composite gels of wheat flour and high amylose corn starch	31:95	Rheological characteristics of binary composite gels of wheat flour and high amylose corn starch.
28963723	5	100	theme	reduction	968:976	arg1	retrogradation					938:951	the retrogradation	934:951	the retrogradation of amylose and reduction of amylopectin from WF	934:999	Furthermore, the dominance of G' than G″ over the range of LVE and high fracture stress at high level of Hylon VII confirmed the high gel strength which can be attributed to the retrogradation of amylose and reduction of amylopectin from WF.
28963723	13	101	theme	composite	2153:2161	arg1	gel					2163:2165	the binary composite gel	2142:2165	the binary composite gel of WF and Hylon VII starch such as the gel strength, fracture stress, fracture strain and material stiffness which enables us to evaluate the nature of starch gels for further applications such as drug delivery systems, elderly diet and further processes	2142:2420	Therefore, the current work was accomplished to provide some knowledge about the binary composite gel of WF and Hylon VII starch such as the gel strength, fracture stress, fracture strain and material stiffness which enables us to evaluate the nature of starch gels for further applications such as drug delivery systems, elderly diet and further processes.
28963723	12	102	theme	strength	1969:1976	arg1	network					1978:1984	a more gel strength network	1958:1984	a more gel strength network	1958:1984	On the contrary, supplying a more gel strength network, which can withstand at high thermal processing and not collapse, is so vital.
28963723	13	103	theme	starch	2319:2324	arg1	gels					2326:2329	starch gels	2319:2329	starch gels	2319:2329	Therefore, the current work was accomplished to provide some knowledge about the binary composite gel of WF and Hylon VII starch such as the gel strength, fracture stress, fracture strain and material stiffness which enables us to evaluate the nature of starch gels for further applications such as drug delivery systems, elderly diet and further processes.
28963723	7	104	theme	lower	1153:1157	arg1	number					1159:1164	a lower number	1151:1164	a lower number of interactions within the gel network structures	1151:1214	High value of α revealed a lower number of interactions within the gel network structures.
28963723	8	105	theme	retort	1331:1336	arg1	processing					1338:1347	retort processing	1331:1347	retort processing	1331:1347	Consequently, due to the high gel strength of BCG of WF/H, it can be exploited at high thermal operations such as retort processing as well as it can be utilized for dysphagia therapy due to the special textural parameters.
28963723	2	106	from	frequency	479:487	arg1	mode					535:538	compression mode	523:538	compression mode	523:538	To this end, the composite gels at different ratios of WF/H including 95:5, 90:10, and 85:15 were tested under a linear viscoelastic regime (LVE) in oscillatory angular frequency and a texture profile analysis in compression mode.
28963723	1	107	dep	starch	142:147	arg1	VII					156:158	Hylon VII	150:158	Hylon VII	150:158	The effect of addition of high amylose corn starch (Hylon VII or H) to wheat flour (WF) on the mechanical properties of the resulting binary composite gels (BCG) under small and large deformations was evaluated.
28963723	1	107	dep	starch	142:147	arg1	H					163:163	H	163:163	H	163:163	The effect of addition of high amylose corn starch (Hylon VII or H) to wheat flour (WF) on the mechanical properties of the resulting binary composite gels (BCG) under small and large deformations was evaluated.
28963723	11	108	theme	desired	1695:1701	arg1	texture					1703:1709	the desired texture	1691:1709	the desired texture of foods	1691:1718	Since, achieving the desired texture of foods has a chief impact on the target consumers, and chewing and swallowing disorders such as dysphagia are common problems in older people, more effort is needed to modify the texture of food with a soft structure.
28963723	0	109	theme	binary	31:36	arg1	gels					48:51	binary composite gels	31:51	binary composite gels of wheat flour and high amylose corn starch	31:95	Rheological characteristics of binary composite gels of wheat flour and high amylose corn starch.
28963723	11	110	theme	soft	1915:1918	arg1	structure					1920:1928	a soft structure	1913:1928	a soft structure	1913:1928	Since, achieving the desired texture of foods has a chief impact on the target consumers, and chewing and swallowing disorders such as dysphagia are common problems in older people, more effort is needed to modify the texture of food with a soft structure.
28963723	10	111	theme	matrices	1664:1671	arg1	properties					1642:1651	the textural properties	1629:1651	the textural properties of the gel matrices	1629:1671	The former gets knowledge about dynamic rheology and the latter gives the textural properties of the gel matrices.
28963723	12	112	theme	more	1960:1963	arg1	network					1978:1984	a more gel strength network	1958:1984	a more gel strength network	1958:1984	On the contrary, supplying a more gel strength network, which can withstand at high thermal processing and not collapse, is so vital.
28963723	2	113	theme	composite	327:335	arg1	90:10					386:390	90:10	386:390	90:10	386:390	To this end, the composite gels at different ratios of WF/H including 95:5, 90:10, and 85:15 were tested under a linear viscoelastic regime (LVE) in oscillatory angular frequency and a texture profile analysis in compression mode.
28963723	2	113	theme	composite	327:335	arg1	85:15					397:401	85:15	397:401	85:15	397:401	To this end, the composite gels at different ratios of WF/H including 95:5, 90:10, and 85:15 were tested under a linear viscoelastic regime (LVE) in oscillatory angular frequency and a texture profile analysis in compression mode.
28963723	2	113	theme	composite	327:335	arg1	95:5					380:383	95:5	380:383	95:5	380:383	To this end, the composite gels at different ratios of WF/H including 95:5, 90:10, and 85:15 were tested under a linear viscoelastic regime (LVE) in oscillatory angular frequency and a texture profile analysis in compression mode.
28963723	2	113	theme	composite	327:335	arg1	gels					337:340	the composite gels	323:340	the composite gels at different ratios of WF/H including 95:5, 90:10, and 85:15	323:401	To this end, the composite gels at different ratios of WF/H including 95:5, 90:10, and 85:15 were tested under a linear viscoelastic regime (LVE) in oscillatory angular frequency and a texture profile analysis in compression mode.
28963723	0	114	theme	gels	48:51	arg1	characteristics					12:26	Rheological characteristics	0:26	Rheological characteristics of binary composite gels of wheat flour and high amylose corn starch.	0:96	Rheological characteristics of binary composite gels of wheat flour and high amylose corn starch.
28963723	1	115	theme	binary	232:237	arg1	gels					249:252	the resulting binary composite gels	218:252	the resulting binary composite gels (BCG) under small and large deformations	218:293	The effect of addition of high amylose corn starch (Hylon VII or H) to wheat flour (WF) on the mechanical properties of the resulting binary composite gels (BCG) under small and large deformations was evaluated.
28963723	1	115	theme	binary	232:237	arg1	BCG					255:257	BCG	255:257	BCG	255:257	The effect of addition of high amylose corn starch (Hylon VII or H) to wheat flour (WF) on the mechanical properties of the resulting binary composite gels (BCG) under small and large deformations was evaluated.
28963723	7	116	theme	High	1126:1129	arg1	value					1131:1135	High value	1126:1135	High value of α	1126:1140	High value of α revealed a lower number of interactions within the gel network structures.
28963723	0	117	theme	wheat	56:60	arg1	flour					62:66	wheat flour	56:66	wheat flour	56:66	Rheological characteristics of binary composite gels of wheat flour and high amylose corn starch.
28963723	1	118	theme	gels	249:252	arg1	properties					204:213	the mechanical properties	189:213	the mechanical properties of the resulting binary composite gels (BCG) under small and large deformations	189:293	The effect of addition of high amylose corn starch (Hylon VII or H) to wheat flour (WF) on the mechanical properties of the resulting binary composite gels (BCG) under small and large deformations was evaluated.
28963723	5	119	theme	Hylon	865:869	arg1	VII					871:873	Hylon VII	865:873	Hylon VII	865:873	Furthermore, the dominance of G' than G″ over the range of LVE and high fracture stress at high level of Hylon VII confirmed the high gel strength which can be attributed to the retrogradation of amylose and reduction of amylopectin from WF.
28963723	12	120	theme	high	2010:2013	arg1	processing					2023:2032	high thermal processing	2010:2032	high thermal processing	2010:2032	On the contrary, supplying a more gel strength network, which can withstand at high thermal processing and not collapse, is so vital.
28963723	11	121	with	texture	1892:1898	arg1	structure					1920:1928	a soft structure	1913:1928	a soft structure	1913:1928	Since, achieving the desired texture of foods has a chief impact on the target consumers, and chewing and swallowing disorders such as dysphagia are common problems in older people, more effort is needed to modify the texture of food with a soft structure.
28963723	2	122	theme	WF/H	365:368	arg1	ratios					355:360	different ratios	345:360	different ratios of WF/H	345:368	To this end, the composite gels at different ratios of WF/H including 95:5, 90:10, and 85:15 were tested under a linear viscoelastic regime (LVE) in oscillatory angular frequency and a texture profile analysis in compression mode.
28963723	3	123	theme	gel	554:556	arg1	firmness					558:565	the gel firmness	550:565	the gel firmness	550:565	However, the gel firmness was increased by Hylon VII addition, but the springiness was reduced.
28963723	5	124	theme	fracture	832:839	arg1	stress					841:846	LVE and high fracture stress	819:846	LVE and high fracture stress	819:846	Furthermore, the dominance of G' than G″ over the range of LVE and high fracture stress at high level of Hylon VII confirmed the high gel strength which can be attributed to the retrogradation of amylose and reduction of amylopectin from WF.
28963723	8	125	theme	dysphagia	1383:1391	arg1	therapy					1393:1399	dysphagia therapy	1383:1399	dysphagia therapy due to the special textural parameters	1383:1438	Consequently, due to the high gel strength of BCG of WF/H, it can be exploited at high thermal operations such as retort processing as well as it can be utilized for dysphagia therapy due to the special textural parameters.
28963723	13	126	theme	WF	2170:2171	arg1	starch					2187:2192	WF and Hylon VII starch	2170:2192	starch	2187:2192	Therefore, the current work was accomplished to provide some knowledge about the binary composite gel of WF and Hylon VII starch such as the gel strength, fracture stress, fracture strain and material stiffness which enables us to evaluate the nature of starch gels for further applications such as drug delivery systems, elderly diet and further processes.
28963723	11	127	theme	chewing	1768:1774	arg1	disorders					1791:1799	chewing and swallowing disorders	1768:1799	chewing and swallowing disorders such as dysphagia	1768:1817	Since, achieving the desired texture of foods has a chief impact on the target consumers, and chewing and swallowing disorders such as dysphagia are common problems in older people, more effort is needed to modify the texture of food with a soft structure.
28963723	11	127	theme	chewing	1768:1774	arg1	dysphagia					1809:1817	dysphagia	1809:1817	dysphagia	1809:1817	Since, achieving the desired texture of foods has a chief impact on the target consumers, and chewing and swallowing disorders such as dysphagia are common problems in older people, more effort is needed to modify the texture of food with a soft structure.
28963723	11	127	theme	chewing	1768:1774	arg1	problems					1830:1837	common problems	1823:1837	common problems in older people	1823:1853	Since, achieving the desired texture of foods has a chief impact on the target consumers, and chewing and swallowing disorders such as dysphagia are common problems in older people, more effort is needed to modify the texture of food with a soft structure.
28963723	13	128	theme	delivery	2369:2376	arg1	systems					2378:2384	drug delivery systems	2364:2384	drug delivery systems	2364:2384	Therefore, the current work was accomplished to provide some knowledge about the binary composite gel of WF and Hylon VII starch such as the gel strength, fracture stress, fracture strain and material stiffness which enables us to evaluate the nature of starch gels for further applications such as drug delivery systems, elderly diet and further processes.
28963723	8	129	theme	gel	1247:1249	arg1	strength					1251:1258	the high gel strength	1238:1258	the high gel strength of BCG of WF/H	1238:1273	Consequently, due to the high gel strength of BCG of WF/H, it can be exploited at high thermal operations such as retort processing as well as it can be utilized for dysphagia therapy due to the special textural parameters.
28963723	13	130	theme	further	2404:2410	arg1	processes					2412:2420	further processes	2404:2420	further processes	2404:2420	Therefore, the current work was accomplished to provide some knowledge about the binary composite gel of WF and Hylon VII starch such as the gel strength, fracture stress, fracture strain and material stiffness which enables us to evaluate the nature of starch gels for further applications such as drug delivery systems, elderly diet and further processes.
25468375	5	0	theme	drug	852:855	arg1	delivery					857:864	the intracellular drug delivery	834:864	the intracellular drug delivery	834:864	To facilitate the intracellular drug delivery, a nona-arginine cell-penetrating peptide (CPP) was chemically conjugated onto the surface of the nanocomposites by oxime click chemistry.
25468375	1	1	theme	microfluidic	349:360	arg1	approach					376:383	a one-step microfluidic self-assembly approach	338:383	a one-step microfluidic self-assembly approach	338:383	An advanced nanocomposite consisting of an encapsulated porous silicon (PSi) nanoparticle and an acid-degradable acetalated dextran (AcDX) matrix (nano-in-nano), was efficiently fabricated by a one-step microfluidic self-assembly approach.
25468375	7	2	theme	polymer	1228:1234	arg1	platform					1313:1320	a universal platform	1301:1320	a universal platform for nanoparticles encapsulation and precisely controlled combination chemotherapy	1301:1402	Overall, this nano-in-nano PSi@polymer composite prepared by the microfluidic self-assembly approach is a universal platform for nanoparticles encapsulation and precisely controlled combination chemotherapy.
25468375	7	2	theme	polymer	1228:1234	arg1	composite					1236:1244	this nano-in-nano PSi@polymer composite	1206:1244	this nano-in-nano PSi@polymer composite prepared by the microfluidic self-assembly approach	1206:1296	Overall, this nano-in-nano PSi@polymer composite prepared by the microfluidic self-assembly approach is a universal platform for nanoparticles encapsulation and precisely controlled combination chemotherapy.
25468375	3	3	theme	multiple	557:564	arg1	drugs					566:570	multiple drugs	557:570	multiple drugs with different physicochemical properties	557:612	Furthermore, multiple drugs with different physicochemical properties have been simultaneously loaded into the nanocomposites with a ratiometric control.
25468375	1	4	theme	AcDX	279:282	arg1	nano-in-nano					293:304	nano-in-nano	293:304	nano-in-nano	293:304	An advanced nanocomposite consisting of an encapsulated porous silicon (PSi) nanoparticle and an acid-degradable acetalated dextran (AcDX) matrix (nano-in-nano), was efficiently fabricated by a one-step microfluidic self-assembly approach.
25468375	1	4	theme	AcDX	279:282	arg1	matrix					285:290	an acid-degradable acetalated dextran (AcDX) matrix	240:290	an acid-degradable acetalated dextran (AcDX) matrix (nano-in-nano)	240:305	An advanced nanocomposite consisting of an encapsulated porous silicon (PSi) nanoparticle and an acid-degradable acetalated dextran (AcDX) matrix (nano-in-nano), was efficiently fabricated by a one-step microfluidic self-assembly approach.
25468375	7	5	theme	controlled	1368:1377	arg1	nanoparticles					1326:1338	nanoparticles encapsulation and precisely controlled combination chemotherapy	1326:1402	nanoparticles encapsulation and precisely controlled combination chemotherapy	1326:1402	Overall, this nano-in-nano PSi@polymer composite prepared by the microfluidic self-assembly approach is a universal platform for nanoparticles encapsulation and precisely controlled combination chemotherapy.
25468375	7	5	theme	controlled	1368:1377	arg1	chemotherapy					1391:1402	precisely controlled combination chemotherapy	1358:1402	precisely controlled combination chemotherapy	1358:1402	Overall, this nano-in-nano PSi@polymer composite prepared by the microfluidic self-assembly approach is a universal platform for nanoparticles encapsulation and precisely controlled combination chemotherapy.
25468375	1	6	theme	self-assembly	362:374	arg1	approach					376:383	a one-step microfluidic self-assembly approach	338:383	a one-step microfluidic self-assembly approach	338:383	An advanced nanocomposite consisting of an encapsulated porous silicon (PSi) nanoparticle and an acid-degradable acetalated dextran (AcDX) matrix (nano-in-nano), was efficiently fabricated by a one-step microfluidic self-assembly approach.
25468375	3	7	with	drugs	566:570	arg1	properties					603:612	different physicochemical properties	577:612	different physicochemical properties	577:612	Furthermore, multiple drugs with different physicochemical properties have been simultaneously loaded into the nanocomposites with a ratiometric control.
25468375	5	8	theme	oxime	982:986	arg1	chemistry					994:1002	oxime click chemistry	982:1002	oxime click chemistry	982:1002	To facilitate the intracellular drug delivery, a nona-arginine cell-penetrating peptide (CPP) was chemically conjugated onto the surface of the nanocomposites by oxime click chemistry.
25468375	1	9	theme	encapsulated	189:200	arg1	nanoparticle					223:234	an encapsulated porous silicon (PSi) nanoparticle	186:234	an encapsulated porous silicon (PSi) nanoparticle	186:234	An advanced nanocomposite consisting of an encapsulated porous silicon (PSi) nanoparticle and an acid-degradable acetalated dextran (AcDX) matrix (nano-in-nano), was efficiently fabricated by a one-step microfluidic self-assembly approach.
25468375	0	10	theme	controlled	109:118	arg1	chemotherapy					132:143	precisely controlled combination chemotherapy	99:143	precisely controlled combination chemotherapy	99:143	Microfluidic assisted one-step fabrication of porous silicon@acetalated dextran nanocomposites for precisely controlled combination chemotherapy.
25468375	7	11	theme	PSi	1224:1226	arg1	platform					1313:1320	a universal platform	1301:1320	a universal platform for nanoparticles encapsulation and precisely controlled combination chemotherapy	1301:1402	Overall, this nano-in-nano PSi@polymer composite prepared by the microfluidic self-assembly approach is a universal platform for nanoparticles encapsulation and precisely controlled combination chemotherapy.
25468375	7	11	theme	PSi	1224:1226	arg1	composite					1236:1244	this nano-in-nano PSi@polymer composite	1206:1244	this nano-in-nano PSi@polymer composite prepared by the microfluidic self-assembly approach	1206:1296	Overall, this nano-in-nano PSi@polymer composite prepared by the microfluidic self-assembly approach is a universal platform for nanoparticles encapsulation and precisely controlled combination chemotherapy.
25468375	7	12	theme	@	1227:1227	arg1	platform					1313:1320	a universal platform	1301:1320	a universal platform for nanoparticles encapsulation and precisely controlled combination chemotherapy	1301:1402	Overall, this nano-in-nano PSi@polymer composite prepared by the microfluidic self-assembly approach is a universal platform for nanoparticles encapsulation and precisely controlled combination chemotherapy.
25468375	7	12	theme	@	1227:1227	arg1	composite					1236:1244	this nano-in-nano PSi@polymer composite	1206:1244	this nano-in-nano PSi@polymer composite prepared by the microfluidic self-assembly approach	1206:1296	Overall, this nano-in-nano PSi@polymer composite prepared by the microfluidic self-assembly approach is a universal platform for nanoparticles encapsulation and precisely controlled combination chemotherapy.
25468375	4	13	theme	AcDX	807:810	arg1	matrix					812:817	the outer AcDX matrix	797:817	the outer AcDX matrix	797:817	The release kinetics of all the payloads was predominantly controlled by the decomposition rate of the outer AcDX matrix.
25468375	4	14	theme	outer	801:805	arg1	matrix					812:817	the outer AcDX matrix	797:817	the outer AcDX matrix	797:817	The release kinetics of all the payloads was predominantly controlled by the decomposition rate of the outer AcDX matrix.
25468375	7	15	theme	universal	1303:1311	arg1	platform					1313:1320	a universal platform	1301:1320	a universal platform for nanoparticles encapsulation and precisely controlled combination chemotherapy	1301:1402	Overall, this nano-in-nano PSi@polymer composite prepared by the microfluidic self-assembly approach is a universal platform for nanoparticles encapsulation and precisely controlled combination chemotherapy.
25468375	7	15	theme	universal	1303:1311	arg1	composite					1236:1244	this nano-in-nano PSi@polymer composite	1206:1244	this nano-in-nano PSi@polymer composite prepared by the microfluidic self-assembly approach	1206:1296	Overall, this nano-in-nano PSi@polymer composite prepared by the microfluidic self-assembly approach is a universal platform for nanoparticles encapsulation and precisely controlled combination chemotherapy.
25468375	3	16	theme	physicochemical	587:601	arg1	properties					603:612	different physicochemical properties	577:612	different physicochemical properties	577:612	Furthermore, multiple drugs with different physicochemical properties have been simultaneously loaded into the nanocomposites with a ratiometric control.
25468375	6	17	theme	multidrug-loaded	1164:1179	arg1	nanocomposites					1181:1194	the CPP-functionalized multidrug-loaded nanocomposites	1141:1194	the CPP-functionalized multidrug-loaded nanocomposites	1141:1194	Taking advantage of the significantly improved cell uptake, the proliferation of two breast cancer cell lines was markedly inhibited by the CPP-functionalized multidrug-loaded nanocomposites.
25468375	1	18	theme	porous	202:207	arg1	nanoparticle					223:234	an encapsulated porous silicon (PSi) nanoparticle	186:234	an encapsulated porous silicon (PSi) nanoparticle	186:234	An advanced nanocomposite consisting of an encapsulated porous silicon (PSi) nanoparticle and an acid-degradable acetalated dextran (AcDX) matrix (nano-in-nano), was efficiently fabricated by a one-step microfluidic self-assembly approach.
25468375	0	19	theme	combination	120:130	arg1	chemotherapy					132:143	precisely controlled combination chemotherapy	99:143	precisely controlled combination chemotherapy	99:143	Microfluidic assisted one-step fabrication of porous silicon@acetalated dextran nanocomposites for precisely controlled combination chemotherapy.
25468375	5	20	theme	click	988:992	arg1	chemistry					994:1002	oxime click chemistry	982:1002	oxime click chemistry	982:1002	To facilitate the intracellular drug delivery, a nona-arginine cell-penetrating peptide (CPP) was chemically conjugated onto the surface of the nanocomposites by oxime click chemistry.
25468375	2	21	theme	enhanced	516:523	arg1	cytocompatibility					525:541	considerably enhanced cytocompatibility	503:541	considerably enhanced cytocompatibility	503:541	The obtained nano-in-nano PSi@AcDX composites showed improved surface smoothness, homogeneous size distribution, and considerably enhanced cytocompatibility.
25468375	2	22	theme	size	480:483	arg1	distribution					485:496	homogeneous size distribution	468:496	homogeneous size distribution	468:496	The obtained nano-in-nano PSi@AcDX composites showed improved surface smoothness, homogeneous size distribution, and considerably enhanced cytocompatibility.
25468375	1	23	theme	silicon	209:215	arg1	nanoparticle					223:234	an encapsulated porous silicon (PSi) nanoparticle	186:234	an encapsulated porous silicon (PSi) nanoparticle	186:234	An advanced nanocomposite consisting of an encapsulated porous silicon (PSi) nanoparticle and an acid-degradable acetalated dextran (AcDX) matrix (nano-in-nano), was efficiently fabricated by a one-step microfluidic self-assembly approach.
25468375	0	24	theme	assisted	13:20	arg1	fabrication					31:41	Microfluidic assisted one-step fabrication	0:41	Microfluidic assisted one-step fabrication of porous silicon@acetalated dextran	0:78	Microfluidic assisted one-step fabrication of porous silicon@acetalated dextran nanocomposites for precisely controlled combination chemotherapy.
25468375	4	25	theme	payloads	730:737	arg1	kinetics					710:717	The release kinetics	698:717	The release kinetics of all the payloads	698:737	The release kinetics of all the payloads was predominantly controlled by the decomposition rate of the outer AcDX matrix.
25468375	2	26	theme	@	415:415	arg1	composites					421:430	The obtained nano-in-nano PSi@AcDX composites	386:430	The obtained nano-in-nano PSi@AcDX composites	386:430	The obtained nano-in-nano PSi@AcDX composites showed improved surface smoothness, homogeneous size distribution, and considerably enhanced cytocompatibility.
25468375	0	27	theme	Microfluidic	0:11	arg1	fabrication					31:41	Microfluidic assisted one-step fabrication	0:41	Microfluidic assisted one-step fabrication of porous silicon@acetalated dextran	0:78	Microfluidic assisted one-step fabrication of porous silicon@acetalated dextran nanocomposites for precisely controlled combination chemotherapy.
25468375	4	28	theme	matrix	812:817	arg1	rate					789:792	the decomposition rate	771:792	the decomposition rate of the outer AcDX matrix	771:817	The release kinetics of all the payloads was predominantly controlled by the decomposition rate of the outer AcDX matrix.
25468375	6	29	theme	breast	1090:1095	arg1	lines					1109:1113	two breast cancer cell lines	1086:1113	two breast cancer cell lines	1086:1113	Taking advantage of the significantly improved cell uptake, the proliferation of two breast cancer cell lines was markedly inhibited by the CPP-functionalized multidrug-loaded nanocomposites.
25468375	1	30	theme	PSi	218:220	arg1	nanoparticle					223:234	an encapsulated porous silicon (PSi) nanoparticle	186:234	an encapsulated porous silicon (PSi) nanoparticle	186:234	An advanced nanocomposite consisting of an encapsulated porous silicon (PSi) nanoparticle and an acid-degradable acetalated dextran (AcDX) matrix (nano-in-nano), was efficiently fabricated by a one-step microfluidic self-assembly approach.
25468375	2	31	theme	homogeneous	468:478	arg1	distribution					485:496	homogeneous size distribution	468:496	homogeneous size distribution	468:496	The obtained nano-in-nano PSi@AcDX composites showed improved surface smoothness, homogeneous size distribution, and considerably enhanced cytocompatibility.
25468375	5	32	theme	nona-arginine	869:881	arg1	CPP					909:911	CPP	909:911	CPP	909:911	To facilitate the intracellular drug delivery, a nona-arginine cell-penetrating peptide (CPP) was chemically conjugated onto the surface of the nanocomposites by oxime click chemistry.
25468375	5	32	theme	nona-arginine	869:881	arg1	peptide					900:906	a nona-arginine cell-penetrating peptide	867:906	a nona-arginine cell-penetrating peptide (CPP)	867:912	To facilitate the intracellular drug delivery, a nona-arginine cell-penetrating peptide (CPP) was chemically conjugated onto the surface of the nanocomposites by oxime click chemistry.
25468375	0	33	theme	one-step	22:29	arg1	fabrication					31:41	Microfluidic assisted one-step fabrication	0:41	Microfluidic assisted one-step fabrication of porous silicon@acetalated dextran	0:78	Microfluidic assisted one-step fabrication of porous silicon@acetalated dextran nanocomposites for precisely controlled combination chemotherapy.
25468375	6	34	theme	uptake	1057:1062	arg1	advantage					1012:1020	advantage	1012:1020	advantage of the significantly improved cell uptake	1012:1062	Taking advantage of the significantly improved cell uptake, the proliferation of two breast cancer cell lines was markedly inhibited by the CPP-functionalized multidrug-loaded nanocomposites.
25468375	6	35	theme	cell	1104:1107	arg1	lines					1109:1113	two breast cancer cell lines	1086:1113	two breast cancer cell lines	1086:1113	Taking advantage of the significantly improved cell uptake, the proliferation of two breast cancer cell lines was markedly inhibited by the CPP-functionalized multidrug-loaded nanocomposites.
25468375	4	36	theme	decomposition	775:787	arg1	rate					789:792	the decomposition rate	771:792	the decomposition rate of the outer AcDX matrix	771:817	The release kinetics of all the payloads was predominantly controlled by the decomposition rate of the outer AcDX matrix.
25468375	5	37	theme	nanocomposites	964:977	arg1	surface					949:955	the surface	945:955	the surface of the nanocomposites	945:977	To facilitate the intracellular drug delivery, a nona-arginine cell-penetrating peptide (CPP) was chemically conjugated onto the surface of the nanocomposites by oxime click chemistry.
25468375	2	38	theme	surface	448:454	arg1	smoothness					456:465	improved surface smoothness	439:465	improved surface smoothness	439:465	The obtained nano-in-nano PSi@AcDX composites showed improved surface smoothness, homogeneous size distribution, and considerably enhanced cytocompatibility.
25468375	0	39	theme	porous	46:51	arg1	dextran					72:78	porous silicon@acetalated dextran	46:78	porous silicon@acetalated dextran	46:78	Microfluidic assisted one-step fabrication of porous silicon@acetalated dextran nanocomposites for precisely controlled combination chemotherapy.
25468375	6	40	theme	cell	1052:1055	arg1	uptake					1057:1062	the significantly improved cell uptake	1025:1062	the significantly improved cell uptake	1025:1062	Taking advantage of the significantly improved cell uptake, the proliferation of two breast cancer cell lines was markedly inhibited by the CPP-functionalized multidrug-loaded nanocomposites.
25468375	2	41	theme	PSi	412:414	arg1	composites					421:430	The obtained nano-in-nano PSi@AcDX composites	386:430	The obtained nano-in-nano PSi@AcDX composites	386:430	The obtained nano-in-nano PSi@AcDX composites showed improved surface smoothness, homogeneous size distribution, and considerably enhanced cytocompatibility.
25468375	2	42	theme	improved	439:446	arg1	smoothness					456:465	improved surface smoothness	439:465	improved surface smoothness	439:465	The obtained nano-in-nano PSi@AcDX composites showed improved surface smoothness, homogeneous size distribution, and considerably enhanced cytocompatibility.
25468375	7	43	theme	microfluidic	1262:1273	arg1	approach					1289:1296	the microfluidic self-assembly approach	1258:1296	the microfluidic self-assembly approach	1258:1296	Overall, this nano-in-nano PSi@polymer composite prepared by the microfluidic self-assembly approach is a universal platform for nanoparticles encapsulation and precisely controlled combination chemotherapy.
25468375	6	44	theme	improved	1043:1050	arg1	uptake					1057:1062	the significantly improved cell uptake	1025:1062	the significantly improved cell uptake	1025:1062	Taking advantage of the significantly improved cell uptake, the proliferation of two breast cancer cell lines was markedly inhibited by the CPP-functionalized multidrug-loaded nanocomposites.
25468375	3	45	theme	ratiometric	677:687	arg1	control					689:695	a ratiometric control	675:695	a ratiometric control	675:695	Furthermore, multiple drugs with different physicochemical properties have been simultaneously loaded into the nanocomposites with a ratiometric control.
25468375	0	46	theme	@	60:60	arg1	dextran					72:78	porous silicon@acetalated dextran	46:78	porous silicon@acetalated dextran	46:78	Microfluidic assisted one-step fabrication of porous silicon@acetalated dextran nanocomposites for precisely controlled combination chemotherapy.
25468375	3	47	with	nanocomposites	655:668	arg1	control					689:695	a ratiometric control	675:695	a ratiometric control	675:695	Furthermore, multiple drugs with different physicochemical properties have been simultaneously loaded into the nanocomposites with a ratiometric control.
25468375	5	48	theme	cell-penetrating	883:898	arg1	CPP					909:911	CPP	909:911	CPP	909:911	To facilitate the intracellular drug delivery, a nona-arginine cell-penetrating peptide (CPP) was chemically conjugated onto the surface of the nanocomposites by oxime click chemistry.
25468375	5	48	theme	cell-penetrating	883:898	arg1	peptide					900:906	a nona-arginine cell-penetrating peptide	867:906	a nona-arginine cell-penetrating peptide (CPP)	867:912	To facilitate the intracellular drug delivery, a nona-arginine cell-penetrating peptide (CPP) was chemically conjugated onto the surface of the nanocomposites by oxime click chemistry.
25468375	5	49	theme	intracellular	838:850	arg1	delivery					857:864	the intracellular drug delivery	834:864	the intracellular drug delivery	834:864	To facilitate the intracellular drug delivery, a nona-arginine cell-penetrating peptide (CPP) was chemically conjugated onto the surface of the nanocomposites by oxime click chemistry.
25468375	0	50	theme	silicon	53:59	arg1	dextran					72:78	porous silicon@acetalated dextran	46:78	porous silicon@acetalated dextran	46:78	Microfluidic assisted one-step fabrication of porous silicon@acetalated dextran nanocomposites for precisely controlled combination chemotherapy.
25468375	7	51	theme	nano-in-nano	1211:1222	arg1	platform					1313:1320	a universal platform	1301:1320	a universal platform for nanoparticles encapsulation and precisely controlled combination chemotherapy	1301:1402	Overall, this nano-in-nano PSi@polymer composite prepared by the microfluidic self-assembly approach is a universal platform for nanoparticles encapsulation and precisely controlled combination chemotherapy.
25468375	7	51	theme	nano-in-nano	1211:1222	arg1	composite					1236:1244	this nano-in-nano PSi@polymer composite	1206:1244	this nano-in-nano PSi@polymer composite prepared by the microfluidic self-assembly approach	1206:1296	Overall, this nano-in-nano PSi@polymer composite prepared by the microfluidic self-assembly approach is a universal platform for nanoparticles encapsulation and precisely controlled combination chemotherapy.
25468375	7	52	theme	self-assembly	1275:1287	arg1	approach					1289:1296	the microfluidic self-assembly approach	1258:1296	the microfluidic self-assembly approach	1258:1296	Overall, this nano-in-nano PSi@polymer composite prepared by the microfluidic self-assembly approach is a universal platform for nanoparticles encapsulation and precisely controlled combination chemotherapy.
25468375	6	53	theme	lines	1109:1113	arg1	proliferation					1069:1081	the proliferation	1065:1081	the proliferation of two breast cancer cell lines	1065:1113	Taking advantage of the significantly improved cell uptake, the proliferation of two breast cancer cell lines was markedly inhibited by the CPP-functionalized multidrug-loaded nanocomposites.
25468375	1	54	theme	advanced	149:156	arg1	nanocomposite					158:170	An advanced nanocomposite	146:170	An advanced nanocomposite	146:170	An advanced nanocomposite consisting of an encapsulated porous silicon (PSi) nanoparticle and an acid-degradable acetalated dextran (AcDX) matrix (nano-in-nano), was efficiently fabricated by a one-step microfluidic self-assembly approach.
25468375	1	55	theme	acid-degradable	243:257	arg1	nano-in-nano					293:304	nano-in-nano	293:304	nano-in-nano	293:304	An advanced nanocomposite consisting of an encapsulated porous silicon (PSi) nanoparticle and an acid-degradable acetalated dextran (AcDX) matrix (nano-in-nano), was efficiently fabricated by a one-step microfluidic self-assembly approach.
25468375	1	55	theme	acid-degradable	243:257	arg1	matrix					285:290	an acid-degradable acetalated dextran (AcDX) matrix	240:290	an acid-degradable acetalated dextran (AcDX) matrix (nano-in-nano)	240:305	An advanced nanocomposite consisting of an encapsulated porous silicon (PSi) nanoparticle and an acid-degradable acetalated dextran (AcDX) matrix (nano-in-nano), was efficiently fabricated by a one-step microfluidic self-assembly approach.
25468375	7	56	dep	nanoparticles	1326:1338	arg1	nanoparticles					1326:1338	nanoparticles encapsulation and precisely controlled combination chemotherapy	1326:1402	nanoparticles encapsulation and precisely controlled combination chemotherapy	1326:1402	Overall, this nano-in-nano PSi@polymer composite prepared by the microfluidic self-assembly approach is a universal platform for nanoparticles encapsulation and precisely controlled combination chemotherapy.
25468375	7	56	dep	nanoparticles	1326:1338	arg1	chemotherapy					1391:1402	precisely controlled combination chemotherapy	1358:1402	precisely controlled combination chemotherapy	1358:1402	Overall, this nano-in-nano PSi@polymer composite prepared by the microfluidic self-assembly approach is a universal platform for nanoparticles encapsulation and precisely controlled combination chemotherapy.
25468375	7	56	dep	nanoparticles	1326:1338	arg1	encapsulation					1340:1352	encapsulation	1340:1352	encapsulation	1340:1352	Overall, this nano-in-nano PSi@polymer composite prepared by the microfluidic self-assembly approach is a universal platform for nanoparticles encapsulation and precisely controlled combination chemotherapy.
25468375	6	57	theme	CPP-functionalized	1145:1162	arg1	nanocomposites					1181:1194	the CPP-functionalized multidrug-loaded nanocomposites	1141:1194	the CPP-functionalized multidrug-loaded nanocomposites	1141:1194	Taking advantage of the significantly improved cell uptake, the proliferation of two breast cancer cell lines was markedly inhibited by the CPP-functionalized multidrug-loaded nanocomposites.
25468375	3	58	theme	different	577:585	arg1	properties					603:612	different physicochemical properties	577:612	different physicochemical properties	577:612	Furthermore, multiple drugs with different physicochemical properties have been simultaneously loaded into the nanocomposites with a ratiometric control.
25468375	1	59	theme	acetalated	259:268	arg1	nano-in-nano					293:304	nano-in-nano	293:304	nano-in-nano	293:304	An advanced nanocomposite consisting of an encapsulated porous silicon (PSi) nanoparticle and an acid-degradable acetalated dextran (AcDX) matrix (nano-in-nano), was efficiently fabricated by a one-step microfluidic self-assembly approach.
25468375	1	59	theme	acetalated	259:268	arg1	matrix					285:290	an acid-degradable acetalated dextran (AcDX) matrix	240:290	an acid-degradable acetalated dextran (AcDX) matrix (nano-in-nano)	240:305	An advanced nanocomposite consisting of an encapsulated porous silicon (PSi) nanoparticle and an acid-degradable acetalated dextran (AcDX) matrix (nano-in-nano), was efficiently fabricated by a one-step microfluidic self-assembly approach.
25468375	0	60	theme	dextran	72:78	arg1	fabrication					31:41	Microfluidic assisted one-step fabrication	0:41	Microfluidic assisted one-step fabrication of porous silicon@acetalated dextran	0:78	Microfluidic assisted one-step fabrication of porous silicon@acetalated dextran nanocomposites for precisely controlled combination chemotherapy.
25468375	4	61	theme	release	702:708	arg1	kinetics					710:717	The release kinetics	698:717	The release kinetics of all the payloads	698:737	The release kinetics of all the payloads was predominantly controlled by the decomposition rate of the outer AcDX matrix.
25468375	2	62	theme	nano-in-nano	399:410	arg1	composites					421:430	The obtained nano-in-nano PSi@AcDX composites	386:430	The obtained nano-in-nano PSi@AcDX composites	386:430	The obtained nano-in-nano PSi@AcDX composites showed improved surface smoothness, homogeneous size distribution, and considerably enhanced cytocompatibility.
25468375	2	63	theme	AcDX	416:419	arg1	composites					421:430	The obtained nano-in-nano PSi@AcDX composites	386:430	The obtained nano-in-nano PSi@AcDX composites	386:430	The obtained nano-in-nano PSi@AcDX composites showed improved surface smoothness, homogeneous size distribution, and considerably enhanced cytocompatibility.
25468375	1	64	theme	dextran	270:276	arg1	nano-in-nano					293:304	nano-in-nano	293:304	nano-in-nano	293:304	An advanced nanocomposite consisting of an encapsulated porous silicon (PSi) nanoparticle and an acid-degradable acetalated dextran (AcDX) matrix (nano-in-nano), was efficiently fabricated by a one-step microfluidic self-assembly approach.
25468375	1	64	theme	dextran	270:276	arg1	matrix					285:290	an acid-degradable acetalated dextran (AcDX) matrix	240:290	an acid-degradable acetalated dextran (AcDX) matrix (nano-in-nano)	240:305	An advanced nanocomposite consisting of an encapsulated porous silicon (PSi) nanoparticle and an acid-degradable acetalated dextran (AcDX) matrix (nano-in-nano), was efficiently fabricated by a one-step microfluidic self-assembly approach.
25468375	0	65	theme	acetalated	61:70	arg1	dextran					72:78	porous silicon@acetalated dextran	46:78	porous silicon@acetalated dextran	46:78	Microfluidic assisted one-step fabrication of porous silicon@acetalated dextran nanocomposites for precisely controlled combination chemotherapy.
25468375	1	66	theme	one-step	340:347	arg1	approach					376:383	a one-step microfluidic self-assembly approach	338:383	a one-step microfluidic self-assembly approach	338:383	An advanced nanocomposite consisting of an encapsulated porous silicon (PSi) nanoparticle and an acid-degradable acetalated dextran (AcDX) matrix (nano-in-nano), was efficiently fabricated by a one-step microfluidic self-assembly approach.
25468375	2	67	theme	obtained	390:397	arg1	composites					421:430	The obtained nano-in-nano PSi@AcDX composites	386:430	The obtained nano-in-nano PSi@AcDX composites	386:430	The obtained nano-in-nano PSi@AcDX composites showed improved surface smoothness, homogeneous size distribution, and considerably enhanced cytocompatibility.
25468375	7	68	theme	combination	1379:1389	arg1	nanoparticles					1326:1338	nanoparticles encapsulation and precisely controlled combination chemotherapy	1326:1402	nanoparticles encapsulation and precisely controlled combination chemotherapy	1326:1402	Overall, this nano-in-nano PSi@polymer composite prepared by the microfluidic self-assembly approach is a universal platform for nanoparticles encapsulation and precisely controlled combination chemotherapy.
25468375	7	68	theme	combination	1379:1389	arg1	chemotherapy					1391:1402	precisely controlled combination chemotherapy	1358:1402	precisely controlled combination chemotherapy	1358:1402	Overall, this nano-in-nano PSi@polymer composite prepared by the microfluidic self-assembly approach is a universal platform for nanoparticles encapsulation and precisely controlled combination chemotherapy.
25468375	6	69	theme	cancer	1097:1102	arg1	lines					1109:1113	two breast cancer cell lines	1086:1113	two breast cancer cell lines	1086:1113	Taking advantage of the significantly improved cell uptake, the proliferation of two breast cancer cell lines was markedly inhibited by the CPP-functionalized multidrug-loaded nanocomposites.
25002135	7	0	theme	species	1089:1095	arg1	isolate					1046:1052	The new isolate	1038:1052	The new isolate	1038:1052	The new isolate and the type strains of recognized species of the genus Flavobacterium could strongly be distinguished by a number of phenotypic characteristics.
25002135	7	0	theme	species	1089:1095	arg1	strains					1067:1073	the type strains	1058:1073	the type strains of recognized species of the genus Flavobacterium	1058:1123	The new isolate and the type strains of recognized species of the genus Flavobacterium could strongly be distinguished by a number of phenotypic characteristics.
25002135	7	1	theme	Flavobacterium	1110:1123	arg1	species					1089:1095	recognized species	1078:1095	recognized species of the genus Flavobacterium	1078:1123	The new isolate and the type strains of recognized species of the genus Flavobacterium could strongly be distinguished by a number of phenotypic characteristics.
25002135	3	2	theme	ponti	669:673	arg1	T					686:686	T	686:686	T	686:686	The phylogenetic analysis based on 16S rRNA gene sequence placed the novel strain within the genus Flavobacterium, the type genus of the family Flavobacteriaceae, the phylum Bacteroidetes, with sequence similarities of 96.2 and 95.7 % to Flavobacterium jumunjiense KCTC 23618(T) and Flavobacterium ponti CCUG 58402(T), and 95.3-92.5 % to other recognized Flavobacterium species.
25002135	3	2	theme	ponti	669:673	arg1	CCUG					675:678	Flavobacterium ponti CCUG 58402	654:684	Flavobacterium ponti CCUG 58402(T)	654:687	The phylogenetic analysis based on 16S rRNA gene sequence placed the novel strain within the genus Flavobacterium, the type genus of the family Flavobacteriaceae, the phylum Bacteroidetes, with sequence similarities of 96.2 and 95.7 % to Flavobacterium jumunjiense KCTC 23618(T) and Flavobacterium ponti CCUG 58402(T), and 95.3-92.5 % to other recognized Flavobacterium species.
25002135	4	3	theme	10Alg	786:790	arg1	iso-C15:0					804:812	iso-C15:0	804:812	iso-C15:0	804:812	The prevalent fatty acids of strain 10Alg 130(T) were iso-C15:0, iso-C15:0 3-OH, iso-C17:0 3-OH, C15:0 and iso-C17:1ω9c.
25002135	4	3	theme	10Alg	786:790	arg1	acids					770:774	The prevalent fatty acids	750:774	The prevalent fatty acids of strain 10Alg 130(T)	750:797	The prevalent fatty acids of strain 10Alg 130(T) were iso-C15:0, iso-C15:0 3-OH, iso-C17:0 3-OH, C15:0 and iso-C17:1ω9c.
25002135	3	4	theme	genus	464:468	arg1	genus					495:499	the type genus	486:499	the type genus of the family Flavobacteriaceae, the phylum Bacteroidetes, with sequence similarities of 96.2 and 95.7 % to Flavobacterium jumunjiense KCTC 23618(T) and Flavobacterium ponti CCUG 58402(T), and 95.3-92.5 % to other recognized Flavobacterium species	486:747	The phylogenetic analysis based on 16S rRNA gene sequence placed the novel strain within the genus Flavobacterium, the type genus of the family Flavobacteriaceae, the phylum Bacteroidetes, with sequence similarities of 96.2 and 95.7 % to Flavobacterium jumunjiense KCTC 23618(T) and Flavobacterium ponti CCUG 58402(T), and 95.3-92.5 % to other recognized Flavobacterium species.
25002135	3	4	theme	genus	464:468	arg1	Flavobacterium					470:483	the genus Flavobacterium	460:483	the genus Flavobacterium	460:483	The phylogenetic analysis based on 16S rRNA gene sequence placed the novel strain within the genus Flavobacterium, the type genus of the family Flavobacteriaceae, the phylum Bacteroidetes, with sequence similarities of 96.2 and 95.7 % to Flavobacterium jumunjiense KCTC 23618(T) and Flavobacterium ponti CCUG 58402(T), and 95.3-92.5 % to other recognized Flavobacterium species.
25002135	4	5	theme	prevalent	754:762	arg1	iso-C15:0					804:812	iso-C15:0	804:812	iso-C15:0	804:812	The prevalent fatty acids of strain 10Alg 130(T) were iso-C15:0, iso-C15:0 3-OH, iso-C17:0 3-OH, C15:0 and iso-C17:1ω9c.
25002135	4	5	theme	prevalent	754:762	arg1	acids					770:774	The prevalent fatty acids	750:774	The prevalent fatty acids of strain 10Alg 130(T)	750:797	The prevalent fatty acids of strain 10Alg 130(T) were iso-C15:0, iso-C15:0 3-OH, iso-C17:0 3-OH, C15:0 and iso-C17:1ω9c.
25002135	1	6	theme	marine	42:47	arg1	bacterium					74:82	a new marine polysaccharide-degrading bacterium	36:82	a new marine polysaccharide-degrading bacterium	36:82	nov., a new marine polysaccharide-degrading bacterium isolated from a Pacific red alga.
25002135	1	6	theme	marine	42:47	arg1	nov.					30:33	nov.	30:33	nov.	30:33	nov., a new marine polysaccharide-degrading bacterium isolated from a Pacific red alga.
25002135	3	7	theme	family	508:513	arg1	Bacteroidetes					545:557	the phylum Bacteroidetes	534:557	the phylum Bacteroidetes	534:557	The phylogenetic analysis based on 16S rRNA gene sequence placed the novel strain within the genus Flavobacterium, the type genus of the family Flavobacteriaceae, the phylum Bacteroidetes, with sequence similarities of 96.2 and 95.7 % to Flavobacterium jumunjiense KCTC 23618(T) and Flavobacterium ponti CCUG 58402(T), and 95.3-92.5 % to other recognized Flavobacterium species.
25002135	3	7	theme	family	508:513	arg1	Flavobacteriaceae					515:531	the family Flavobacteriaceae	504:531	the family Flavobacteriaceae	504:531	The phylogenetic analysis based on 16S rRNA gene sequence placed the novel strain within the genus Flavobacterium, the type genus of the family Flavobacteriaceae, the phylum Bacteroidetes, with sequence similarities of 96.2 and 95.7 % to Flavobacterium jumunjiense KCTC 23618(T) and Flavobacterium ponti CCUG 58402(T), and 95.3-92.5 % to other recognized Flavobacterium species.
25002135	7	8	theme	recognized	1078:1087	arg1	species					1089:1095	recognized species	1078:1095	recognized species of the genus Flavobacterium	1078:1123	The new isolate and the type strains of recognized species of the genus Flavobacterium could strongly be distinguished by a number of phenotypic characteristics.
25002135	2	9	theme	rod-shaped	144:153	arg1	bacterium					195:203	A Gram-negative, aerobic, rod-shaped, motile by gliding and yellow-pigmented bacterium	118:203	A Gram-negative, aerobic, rod-shaped, motile by gliding and yellow-pigmented bacterium	118:203	A Gram-negative, aerobic, rod-shaped, motile by gliding and yellow-pigmented bacterium, designated strain 10Alg 130(T), that displayed the ability to destroy polysaccharides of red and brown algae, was isolated from the red alga Ahnfeltia tobuchiensis.
25002135	8	10	theme	genotypic	1221:1229	arg1	data					1246:1249	the genotypic and phenotypic data	1217:1249	the genotypic and phenotypic data	1217:1249	A combination of the genotypic and phenotypic data showed that the algal isolate represents a novel species of the genus Flavobacterium, for which the name Flavobacterium ahnfeltiae sp.
25002135	1	11	theme	polysaccharide-degrading	49:72	arg1	bacterium					74:82	a new marine polysaccharide-degrading bacterium	36:82	a new marine polysaccharide-degrading bacterium	36:82	nov., a new marine polysaccharide-degrading bacterium isolated from a Pacific red alga.
25002135	1	11	theme	polysaccharide-degrading	49:72	arg1	nov.					30:33	nov.	30:33	nov.	30:33	nov., a new marine polysaccharide-degrading bacterium isolated from a Pacific red alga.
25002135	3	12	theme	novel	440:444	arg1	strain					446:451	the novel strain	436:451	the novel strain	436:451	The phylogenetic analysis based on 16S rRNA gene sequence placed the novel strain within the genus Flavobacterium, the type genus of the family Flavobacteriaceae, the phylum Bacteroidetes, with sequence similarities of 96.2 and 95.7 % to Flavobacterium jumunjiense KCTC 23618(T) and Flavobacterium ponti CCUG 58402(T), and 95.3-92.5 % to other recognized Flavobacterium species.
25002135	7	13	theme	type	1062:1065	arg1	strains					1067:1073	the type strains	1058:1073	the type strains of recognized species of the genus Flavobacterium	1058:1123	The new isolate and the type strains of recognized species of the genus Flavobacterium could strongly be distinguished by a number of phenotypic characteristics.
25002135	3	14	theme	CCUG	675:678	arg1	similarities					574:585	sequence similarities	565:585	sequence similarities of 96.2 and 95.7 % to Flavobacterium jumunjiense KCTC 23618(T) and Flavobacterium ponti CCUG 58402(T), and 95.3-92.5 % to other recognized Flavobacterium species	565:747	The phylogenetic analysis based on 16S rRNA gene sequence placed the novel strain within the genus Flavobacterium, the type genus of the family Flavobacteriaceae, the phylum Bacteroidetes, with sequence similarities of 96.2 and 95.7 % to Flavobacterium jumunjiense KCTC 23618(T) and Flavobacterium ponti CCUG 58402(T), and 95.3-92.5 % to other recognized Flavobacterium species.
25002135	8	15	theme	data	1246:1249	arg1	combination					1202:1212	A combination	1200:1212	A combination of the genotypic and phenotypic data	1200:1249	A combination of the genotypic and phenotypic data showed that the algal isolate represents a novel species of the genus Flavobacterium, for which the name Flavobacterium ahnfeltiae sp.
25002135	6	16	theme	DNA	988:990	arg1	content					996:1002	The DNA G+C content	984:1002	The DNA G+C content of the type strain	984:1021	The DNA G+C content of the type strain was 34.3 mol%.
25002135	6	16	theme	DNA	988:990	arg1	%					1035:1035	34.3 mol%	1027:1035	34.3 mol%	1027:1035	The DNA G+C content of the type strain was 34.3 mol%.
25002135	8	17	theme	Flavobacterium	1356:1369	arg1	sp					1382:1383	the name Flavobacterium ahnfeltiae sp	1347:1383	the name Flavobacterium ahnfeltiae sp	1347:1383	A combination of the genotypic and phenotypic data showed that the algal isolate represents a novel species of the genus Flavobacterium, for which the name Flavobacterium ahnfeltiae sp.
25002135	2	18	theme	aerobic	135:141	arg1	bacterium					195:203	A Gram-negative, aerobic, rod-shaped, motile by gliding and yellow-pigmented bacterium	118:203	A Gram-negative, aerobic, rod-shaped, motile by gliding and yellow-pigmented bacterium	118:203	A Gram-negative, aerobic, rod-shaped, motile by gliding and yellow-pigmented bacterium, designated strain 10Alg 130(T), that displayed the ability to destroy polysaccharides of red and brown algae, was isolated from the red alga Ahnfeltia tobuchiensis.
25002135	4	19	theme	fatty	764:768	arg1	iso-C15:0					804:812	iso-C15:0	804:812	iso-C15:0	804:812	The prevalent fatty acids of strain 10Alg 130(T) were iso-C15:0, iso-C15:0 3-OH, iso-C17:0 3-OH, C15:0 and iso-C17:1ω9c.
25002135	4	19	theme	fatty	764:768	arg1	acids					770:774	The prevalent fatty acids	750:774	The prevalent fatty acids of strain 10Alg 130(T)	750:797	The prevalent fatty acids of strain 10Alg 130(T) were iso-C15:0, iso-C15:0 3-OH, iso-C17:0 3-OH, C15:0 and iso-C17:1ω9c.
25002135	3	20	theme	type	490:493	arg1	genus					495:499	the type genus	486:499	the type genus of the family Flavobacteriaceae, the phylum Bacteroidetes, with sequence similarities of 96.2 and 95.7 % to Flavobacterium jumunjiense KCTC 23618(T) and Flavobacterium ponti CCUG 58402(T), and 95.3-92.5 % to other recognized Flavobacterium species	486:747	The phylogenetic analysis based on 16S rRNA gene sequence placed the novel strain within the genus Flavobacterium, the type genus of the family Flavobacteriaceae, the phylum Bacteroidetes, with sequence similarities of 96.2 and 95.7 % to Flavobacterium jumunjiense KCTC 23618(T) and Flavobacterium ponti CCUG 58402(T), and 95.3-92.5 % to other recognized Flavobacterium species.
25002135	3	20	theme	type	490:493	arg1	Flavobacterium					470:483	the genus Flavobacterium	460:483	the genus Flavobacterium	460:483	The phylogenetic analysis based on 16S rRNA gene sequence placed the novel strain within the genus Flavobacterium, the type genus of the family Flavobacteriaceae, the phylum Bacteroidetes, with sequence similarities of 96.2 and 95.7 % to Flavobacterium jumunjiense KCTC 23618(T) and Flavobacterium ponti CCUG 58402(T), and 95.3-92.5 % to other recognized Flavobacterium species.
25002135	6	21	theme	G+C	992:994	arg1	content					996:1002	The DNA G+C content	984:1002	The DNA G+C content of the type strain	984:1021	The DNA G+C content of the type strain was 34.3 mol%.
25002135	6	21	theme	G+C	992:994	arg1	%					1035:1035	34.3 mol%	1027:1035	34.3 mol%	1027:1035	The DNA G+C content of the type strain was 34.3 mol%.
25002135	3	22	theme	16S	406:408	arg1	sequence					420:427	16S rRNA gene sequence	406:427	16S rRNA gene sequence	406:427	The phylogenetic analysis based on 16S rRNA gene sequence placed the novel strain within the genus Flavobacterium, the type genus of the family Flavobacteriaceae, the phylum Bacteroidetes, with sequence similarities of 96.2 and 95.7 % to Flavobacterium jumunjiense KCTC 23618(T) and Flavobacterium ponti CCUG 58402(T), and 95.3-92.5 % to other recognized Flavobacterium species.
25002135	0	23	theme	ahnfeltiae	15:24	arg1	sp					26:27	Flavobacterium ahnfeltiae sp	0:27	Flavobacterium ahnfeltiae sp.	0:28	Flavobacterium ahnfeltiae sp.
25002135	3	24	theme	Flavobacterium	654:667	arg1	T					686:686	T	686:686	T	686:686	The phylogenetic analysis based on 16S rRNA gene sequence placed the novel strain within the genus Flavobacterium, the type genus of the family Flavobacteriaceae, the phylum Bacteroidetes, with sequence similarities of 96.2 and 95.7 % to Flavobacterium jumunjiense KCTC 23618(T) and Flavobacterium ponti CCUG 58402(T), and 95.3-92.5 % to other recognized Flavobacterium species.
25002135	3	24	theme	Flavobacterium	654:667	arg1	CCUG					675:678	Flavobacterium ponti CCUG 58402	654:684	Flavobacterium ponti CCUG 58402(T)	654:687	The phylogenetic analysis based on 16S rRNA gene sequence placed the novel strain within the genus Flavobacterium, the type genus of the family Flavobacteriaceae, the phylum Bacteroidetes, with sequence similarities of 96.2 and 95.7 % to Flavobacterium jumunjiense KCTC 23618(T) and Flavobacterium ponti CCUG 58402(T), and 95.3-92.5 % to other recognized Flavobacterium species.
25002135	4	25	theme	strain	779:784	arg1	10Alg					786:790	strain 10Alg 130	779:794	strain 10Alg 130(T)	779:797	The prevalent fatty acids of strain 10Alg 130(T) were iso-C15:0, iso-C15:0 3-OH, iso-C17:0 3-OH, C15:0 and iso-C17:1ω9c.
25002135	4	25	theme	strain	779:784	arg1	T					796:796	T	796:796	T	796:796	The prevalent fatty acids of strain 10Alg 130(T) were iso-C15:0, iso-C15:0 3-OH, iso-C17:0 3-OH, C15:0 and iso-C17:1ω9c.
25002135	3	26	theme	Flavobacteriaceae	515:531	arg1	genus					495:499	the type genus	486:499	the type genus of the family Flavobacteriaceae, the phylum Bacteroidetes, with sequence similarities of 96.2 and 95.7 % to Flavobacterium jumunjiense KCTC 23618(T) and Flavobacterium ponti CCUG 58402(T), and 95.3-92.5 % to other recognized Flavobacterium species	486:747	The phylogenetic analysis based on 16S rRNA gene sequence placed the novel strain within the genus Flavobacterium, the type genus of the family Flavobacteriaceae, the phylum Bacteroidetes, with sequence similarities of 96.2 and 95.7 % to Flavobacterium jumunjiense KCTC 23618(T) and Flavobacterium ponti CCUG 58402(T), and 95.3-92.5 % to other recognized Flavobacterium species.
25002135	3	26	theme	Flavobacteriaceae	515:531	arg1	Flavobacterium					470:483	the genus Flavobacterium	460:483	the genus Flavobacterium	460:483	The phylogenetic analysis based on 16S rRNA gene sequence placed the novel strain within the genus Flavobacterium, the type genus of the family Flavobacteriaceae, the phylum Bacteroidetes, with sequence similarities of 96.2 and 95.7 % to Flavobacterium jumunjiense KCTC 23618(T) and Flavobacterium ponti CCUG 58402(T), and 95.3-92.5 % to other recognized Flavobacterium species.
25002135	2	27	theme	red	338:340	arg1	alga					342:345	the red alga	334:345	the red alga	334:345	A Gram-negative, aerobic, rod-shaped, motile by gliding and yellow-pigmented bacterium, designated strain 10Alg 130(T), that displayed the ability to destroy polysaccharides of red and brown algae, was isolated from the red alga Ahnfeltia tobuchiensis.
25002135	0	28	theme	Flavobacterium	0:13	arg1	sp					26:27	Flavobacterium ahnfeltiae sp	0:27	Flavobacterium ahnfeltiae sp.	0:28	Flavobacterium ahnfeltiae sp.
25002135	5	29	theme	unknown	968:974	arg1	lipids					976:981	three unknown lipids	962:981	three unknown lipids	962:981	The polar lipid profile consisted of phosphatidylethanolamine, two unknown aminolipids and three unknown lipids.
25002135	8	30	theme	name	1351:1354	arg1	sp					1382:1383	the name Flavobacterium ahnfeltiae sp	1347:1383	the name Flavobacterium ahnfeltiae sp	1347:1383	A combination of the genotypic and phenotypic data showed that the algal isolate represents a novel species of the genus Flavobacterium, for which the name Flavobacterium ahnfeltiae sp.
25002135	8	31	theme	Flavobacterium	1321:1334	arg1	species					1300:1306	a novel species	1292:1306	a novel species	1292:1306	A combination of the genotypic and phenotypic data showed that the algal isolate represents a novel species of the genus Flavobacterium, for which the name Flavobacterium ahnfeltiae sp.
25002135	3	32	with	Flavobacteriaceae	515:531	arg1	similarities					574:585	sequence similarities	565:585	sequence similarities of 96.2 and 95.7 % to Flavobacterium jumunjiense KCTC 23618(T) and Flavobacterium ponti CCUG 58402(T), and 95.3-92.5 % to other recognized Flavobacterium species	565:747	The phylogenetic analysis based on 16S rRNA gene sequence placed the novel strain within the genus Flavobacterium, the type genus of the family Flavobacteriaceae, the phylum Bacteroidetes, with sequence similarities of 96.2 and 95.7 % to Flavobacterium jumunjiense KCTC 23618(T) and Flavobacterium ponti CCUG 58402(T), and 95.3-92.5 % to other recognized Flavobacterium species.
25002135	6	33	theme	mol	1032:1034	arg1	%					1035:1035	34.3 mol%	1027:1035	34.3 mol%	1027:1035	The DNA G+C content of the type strain was 34.3 mol%.
25002135	6	33	theme	mol	1032:1034	arg1	content					996:1002	The DNA G+C content	984:1002	The DNA G+C content of the type strain	984:1021	The DNA G+C content of the type strain was 34.3 mol%.
25002135	1	34	theme	Pacific	100:106	arg1	alga					112:115	a Pacific red alga	98:115	a Pacific red alga	98:115	nov., a new marine polysaccharide-degrading bacterium isolated from a Pacific red alga.
25002135	7	35	theme	characteristics	1183:1197	arg1	number					1162:1167	a number	1160:1167	a number of phenotypic characteristics	1160:1197	The new isolate and the type strains of recognized species of the genus Flavobacterium could strongly be distinguished by a number of phenotypic characteristics.
25002135	6	36	theme	type	1011:1014	arg1	strain					1016:1021	the type strain	1007:1021	the type strain	1007:1021	The DNA G+C content of the type strain was 34.3 mol%.
25002135	4	37	theme	iso-C15:0	815:823	arg1	3-OH					825:828	iso-C15:0 3-OH	815:828	iso-C15:0 3-OH	815:828	The prevalent fatty acids of strain 10Alg 130(T) were iso-C15:0, iso-C15:0 3-OH, iso-C17:0 3-OH, C15:0 and iso-C17:1ω9c.
25002135	1	38	theme	red	108:110	arg1	alga					112:115	a Pacific red alga	98:115	a Pacific red alga	98:115	nov., a new marine polysaccharide-degrading bacterium isolated from a Pacific red alga.
25002135	7	39	theme	phenotypic	1172:1181	arg1	characteristics					1183:1197	phenotypic characteristics	1172:1197	phenotypic characteristics	1172:1197	The new isolate and the type strains of recognized species of the genus Flavobacterium could strongly be distinguished by a number of phenotypic characteristics.
25002135	4	40	theme	iso-C17:0	831:839	arg1	3-OH					841:844	iso-C17:0 3-OH	831:844	iso-C17:0 3-OH	831:844	The prevalent fatty acids of strain 10Alg 130(T) were iso-C15:0, iso-C15:0 3-OH, iso-C17:0 3-OH, C15:0 and iso-C17:1ω9c.
25002135	3	41	theme	%	604:604	arg1	similarities					574:585	sequence similarities	565:585	sequence similarities of 96.2 and 95.7 % to Flavobacterium jumunjiense KCTC 23618(T) and Flavobacterium ponti CCUG 58402(T), and 95.3-92.5 % to other recognized Flavobacterium species	565:747	The phylogenetic analysis based on 16S rRNA gene sequence placed the novel strain within the genus Flavobacterium, the type genus of the family Flavobacteriaceae, the phylum Bacteroidetes, with sequence similarities of 96.2 and 95.7 % to Flavobacterium jumunjiense KCTC 23618(T) and Flavobacterium ponti CCUG 58402(T), and 95.3-92.5 % to other recognized Flavobacterium species.
25002135	3	42	theme	rRNA	410:413	arg1	sequence					420:427	16S rRNA gene sequence	406:427	16S rRNA gene sequence	406:427	The phylogenetic analysis based on 16S rRNA gene sequence placed the novel strain within the genus Flavobacterium, the type genus of the family Flavobacteriaceae, the phylum Bacteroidetes, with sequence similarities of 96.2 and 95.7 % to Flavobacterium jumunjiense KCTC 23618(T) and Flavobacterium ponti CCUG 58402(T), and 95.3-92.5 % to other recognized Flavobacterium species.
25002135	3	43	theme	phylogenetic	375:386	arg1	analysis					388:395	The phylogenetic analysis	371:395	The phylogenetic analysis based on 16S rRNA gene sequence	371:427	The phylogenetic analysis based on 16S rRNA gene sequence placed the novel strain within the genus Flavobacterium, the type genus of the family Flavobacteriaceae, the phylum Bacteroidetes, with sequence similarities of 96.2 and 95.7 % to Flavobacterium jumunjiense KCTC 23618(T) and Flavobacterium ponti CCUG 58402(T), and 95.3-92.5 % to other recognized Flavobacterium species.
25002135	10	44	theme	KMM	1454:1456	arg1	10Alg					1423:1427	10Alg 130	1423:1431	10Alg 130(T) (=KCTC 32467(T) = KMM 6686(T))	1423:1465	The type strain is 10Alg 130(T) (=KCTC 32467(T) = KMM 6686(T)).
25002135	10	44	theme	KMM	1454:1456	arg1	T					1463:1463	T	1463:1463	T	1463:1463	The type strain is 10Alg 130(T) (=KCTC 32467(T) = KMM 6686(T)).
25002135	10	44	theme	KMM	1454:1456	arg1	6686					1458:1461	=KCTC 32467(T) = KMM 6686	1437:1461	=KCTC 32467(T) = KMM 6686(T)	1437:1464	The type strain is 10Alg 130(T) (=KCTC 32467(T) = KMM 6686(T)).
25002135	2	45	theme	motile	156:161	arg1	bacterium					195:203	A Gram-negative, aerobic, rod-shaped, motile by gliding and yellow-pigmented bacterium	118:203	A Gram-negative, aerobic, rod-shaped, motile by gliding and yellow-pigmented bacterium	118:203	A Gram-negative, aerobic, rod-shaped, motile by gliding and yellow-pigmented bacterium, designated strain 10Alg 130(T), that displayed the ability to destroy polysaccharides of red and brown algae, was isolated from the red alga Ahnfeltia tobuchiensis.
25002135	7	46	theme	new	1042:1044	arg1	isolate					1046:1052	The new isolate	1038:1052	The new isolate	1038:1052	The new isolate and the type strains of recognized species of the genus Flavobacterium could strongly be distinguished by a number of phenotypic characteristics.
25002135	3	47	theme	gene	415:418	arg1	sequence					420:427	16S rRNA gene sequence	406:427	16S rRNA gene sequence	406:427	The phylogenetic analysis based on 16S rRNA gene sequence placed the novel strain within the genus Flavobacterium, the type genus of the family Flavobacteriaceae, the phylum Bacteroidetes, with sequence similarities of 96.2 and 95.7 % to Flavobacterium jumunjiense KCTC 23618(T) and Flavobacterium ponti CCUG 58402(T), and 95.3-92.5 % to other recognized Flavobacterium species.
25002135	10	48	theme	=	1452:1452	arg1	10Alg					1423:1427	10Alg 130	1423:1431	10Alg 130(T) (=KCTC 32467(T) = KMM 6686(T))	1423:1465	The type strain is 10Alg 130(T) (=KCTC 32467(T) = KMM 6686(T)).
25002135	10	48	theme	=	1452:1452	arg1	T					1463:1463	T	1463:1463	T	1463:1463	The type strain is 10Alg 130(T) (=KCTC 32467(T) = KMM 6686(T)).
25002135	10	48	theme	=	1452:1452	arg1	6686					1458:1461	=KCTC 32467(T) = KMM 6686	1437:1461	=KCTC 32467(T) = KMM 6686(T)	1437:1464	The type strain is 10Alg 130(T) (=KCTC 32467(T) = KMM 6686(T)).
25002135	3	49	theme	%	704:704	arg1	similarities					574:585	sequence similarities	565:585	sequence similarities of 96.2 and 95.7 % to Flavobacterium jumunjiense KCTC 23618(T) and Flavobacterium ponti CCUG 58402(T), and 95.3-92.5 % to other recognized Flavobacterium species	565:747	The phylogenetic analysis based on 16S rRNA gene sequence placed the novel strain within the genus Flavobacterium, the type genus of the family Flavobacteriaceae, the phylum Bacteroidetes, with sequence similarities of 96.2 and 95.7 % to Flavobacterium jumunjiense KCTC 23618(T) and Flavobacterium ponti CCUG 58402(T), and 95.3-92.5 % to other recognized Flavobacterium species.
25002135	10	50	theme	type	1408:1411	arg1	10Alg					1423:1427	10Alg 130	1423:1431	10Alg 130(T) (=KCTC 32467(T) = KMM 6686(T))	1423:1465	The type strain is 10Alg 130(T) (=KCTC 32467(T) = KMM 6686(T)).
25002135	10	50	theme	type	1408:1411	arg1	strain					1413:1418	The type strain	1404:1418	The type strain	1404:1418	The type strain is 10Alg 130(T) (=KCTC 32467(T) = KMM 6686(T)).
25002135	2	51	theme	strain	217:222	arg1	10Alg					224:228	strain 10Alg 130	217:232	strain 10Alg 130(T)	217:235	A Gram-negative, aerobic, rod-shaped, motile by gliding and yellow-pigmented bacterium, designated strain 10Alg 130(T), that displayed the ability to destroy polysaccharides of red and brown algae, was isolated from the red alga Ahnfeltia tobuchiensis.
25002135	2	51	theme	strain	217:222	arg1	T					234:234	T	234:234	T	234:234	A Gram-negative, aerobic, rod-shaped, motile by gliding and yellow-pigmented bacterium, designated strain 10Alg 130(T), that displayed the ability to destroy polysaccharides of red and brown algae, was isolated from the red alga Ahnfeltia tobuchiensis.
25002135	2	52	theme	algae	309:313	arg1	polysaccharides					276:290	polysaccharides	276:290	polysaccharides of red and brown algae	276:313	A Gram-negative, aerobic, rod-shaped, motile by gliding and yellow-pigmented bacterium, designated strain 10Alg 130(T), that displayed the ability to destroy polysaccharides of red and brown algae, was isolated from the red alga Ahnfeltia tobuchiensis.
25002135	8	53	theme	novel	1294:1298	arg1	species					1300:1306	a novel species	1292:1306	a novel species	1292:1306	A combination of the genotypic and phenotypic data showed that the algal isolate represents a novel species of the genus Flavobacterium, for which the name Flavobacterium ahnfeltiae sp.
25002135	2	54	attach	isolated	320:327	arg1	alga					342:345	the red alga	334:345	the red alga	334:345	A Gram-negative, aerobic, rod-shaped, motile by gliding and yellow-pigmented bacterium, designated strain 10Alg 130(T), that displayed the ability to destroy polysaccharides of red and brown algae, was isolated from the red alga Ahnfeltia tobuchiensis.
25002135	2	54	attach	isolated	320:327	arg2	bacterium					195:203	A Gram-negative, aerobic, rod-shaped, motile by gliding and yellow-pigmented bacterium	118:203	A Gram-negative, aerobic, rod-shaped, motile by gliding and yellow-pigmented bacterium	118:203	A Gram-negative, aerobic, rod-shaped, motile by gliding and yellow-pigmented bacterium, designated strain 10Alg 130(T), that displayed the ability to destroy polysaccharides of red and brown algae, was isolated from the red alga Ahnfeltia tobuchiensis.
25002135	3	55	theme	jumunjiense	624:634	arg1	T					647:647	T	647:647	T	647:647	The phylogenetic analysis based on 16S rRNA gene sequence placed the novel strain within the genus Flavobacterium, the type genus of the family Flavobacteriaceae, the phylum Bacteroidetes, with sequence similarities of 96.2 and 95.7 % to Flavobacterium jumunjiense KCTC 23618(T) and Flavobacterium ponti CCUG 58402(T), and 95.3-92.5 % to other recognized Flavobacterium species.
25002135	3	55	theme	jumunjiense	624:634	arg1	KCTC					636:639	Flavobacterium jumunjiense KCTC 23618	609:645	Flavobacterium jumunjiense KCTC 23618(T)	609:648	The phylogenetic analysis based on 16S rRNA gene sequence placed the novel strain within the genus Flavobacterium, the type genus of the family Flavobacteriaceae, the phylum Bacteroidetes, with sequence similarities of 96.2 and 95.7 % to Flavobacterium jumunjiense KCTC 23618(T) and Flavobacterium ponti CCUG 58402(T), and 95.3-92.5 % to other recognized Flavobacterium species.
25002135	5	56	theme	unknown	938:944	arg1	aminolipids					946:956	two unknown aminolipids	934:956	two unknown aminolipids	934:956	The polar lipid profile consisted of phosphatidylethanolamine, two unknown aminolipids and three unknown lipids.
25002135	2	57	theme	brown	303:307	arg1	algae					309:313	red and brown algae	295:313	algae	309:313	A Gram-negative, aerobic, rod-shaped, motile by gliding and yellow-pigmented bacterium, designated strain 10Alg 130(T), that displayed the ability to destroy polysaccharides of red and brown algae, was isolated from the red alga Ahnfeltia tobuchiensis.
25002135	1	58	theme	new	38:40	arg1	bacterium					74:82	a new marine polysaccharide-degrading bacterium	36:82	a new marine polysaccharide-degrading bacterium	36:82	nov., a new marine polysaccharide-degrading bacterium isolated from a Pacific red alga.
25002135	1	58	theme	new	38:40	arg1	nov.					30:33	nov.	30:33	nov.	30:33	nov., a new marine polysaccharide-degrading bacterium isolated from a Pacific red alga.
25002135	5	59	theme	lipid	881:885	arg1	profile					887:893	The polar lipid profile	871:893	The polar lipid profile	871:893	The polar lipid profile consisted of phosphatidylethanolamine, two unknown aminolipids and three unknown lipids.
25002135	6	60	theme	strain	1016:1021	arg1	content					996:1002	The DNA G+C content	984:1002	The DNA G+C content of the type strain	984:1021	The DNA G+C content of the type strain was 34.3 mol%.
25002135	6	60	theme	strain	1016:1021	arg1	%					1035:1035	34.3 mol%	1027:1035	34.3 mol%	1027:1035	The DNA G+C content of the type strain was 34.3 mol%.
25002135	8	61	theme	phenotypic	1235:1244	arg1	data					1246:1249	the genotypic and phenotypic data	1217:1249	the genotypic and phenotypic data	1217:1249	A combination of the genotypic and phenotypic data showed that the algal isolate represents a novel species of the genus Flavobacterium, for which the name Flavobacterium ahnfeltiae sp.
25002135	3	62	theme	recognized	715:724	arg1	species					741:747	other recognized Flavobacterium species	709:747	other recognized Flavobacterium species	709:747	The phylogenetic analysis based on 16S rRNA gene sequence placed the novel strain within the genus Flavobacterium, the type genus of the family Flavobacteriaceae, the phylum Bacteroidetes, with sequence similarities of 96.2 and 95.7 % to Flavobacterium jumunjiense KCTC 23618(T) and Flavobacterium ponti CCUG 58402(T), and 95.3-92.5 % to other recognized Flavobacterium species.
25002135	5	63	theme	polar	875:879	arg1	profile					887:893	The polar lipid profile	871:893	The polar lipid profile	871:893	The polar lipid profile consisted of phosphatidylethanolamine, two unknown aminolipids and three unknown lipids.
25002135	2	64	theme	yellow-pigmented	178:193	arg1	bacterium					195:203	A Gram-negative, aerobic, rod-shaped, motile by gliding and yellow-pigmented bacterium	118:203	A Gram-negative, aerobic, rod-shaped, motile by gliding and yellow-pigmented bacterium	118:203	A Gram-negative, aerobic, rod-shaped, motile by gliding and yellow-pigmented bacterium, designated strain 10Alg 130(T), that displayed the ability to destroy polysaccharides of red and brown algae, was isolated from the red alga Ahnfeltia tobuchiensis.
25002135	2	65	theme	red	295:297	arg1	algae					309:313	red and brown algae	295:313	algae	309:313	A Gram-negative, aerobic, rod-shaped, motile by gliding and yellow-pigmented bacterium, designated strain 10Alg 130(T), that displayed the ability to destroy polysaccharides of red and brown algae, was isolated from the red alga Ahnfeltia tobuchiensis.
25002135	3	66	theme	Flavobacterium	726:739	arg1	species					741:747	other recognized Flavobacterium species	709:747	other recognized Flavobacterium species	709:747	The phylogenetic analysis based on 16S rRNA gene sequence placed the novel strain within the genus Flavobacterium, the type genus of the family Flavobacteriaceae, the phylum Bacteroidetes, with sequence similarities of 96.2 and 95.7 % to Flavobacterium jumunjiense KCTC 23618(T) and Flavobacterium ponti CCUG 58402(T), and 95.3-92.5 % to other recognized Flavobacterium species.
25002135	3	67	theme	Flavobacterium	609:622	arg1	T					647:647	T	647:647	T	647:647	The phylogenetic analysis based on 16S rRNA gene sequence placed the novel strain within the genus Flavobacterium, the type genus of the family Flavobacteriaceae, the phylum Bacteroidetes, with sequence similarities of 96.2 and 95.7 % to Flavobacterium jumunjiense KCTC 23618(T) and Flavobacterium ponti CCUG 58402(T), and 95.3-92.5 % to other recognized Flavobacterium species.
25002135	3	67	theme	Flavobacterium	609:622	arg1	KCTC					636:639	Flavobacterium jumunjiense KCTC 23618	609:645	Flavobacterium jumunjiense KCTC 23618(T)	609:648	The phylogenetic analysis based on 16S rRNA gene sequence placed the novel strain within the genus Flavobacterium, the type genus of the family Flavobacteriaceae, the phylum Bacteroidetes, with sequence similarities of 96.2 and 95.7 % to Flavobacterium jumunjiense KCTC 23618(T) and Flavobacterium ponti CCUG 58402(T), and 95.3-92.5 % to other recognized Flavobacterium species.
25002135	8	68	theme	genus	1315:1319	arg1	Flavobacterium					1321:1334	the genus Flavobacterium	1311:1334	the genus Flavobacterium	1311:1334	A combination of the genotypic and phenotypic data showed that the algal isolate represents a novel species of the genus Flavobacterium, for which the name Flavobacterium ahnfeltiae sp.
25002135	8	69	theme	algal	1267:1271	arg1	isolate					1273:1279	the algal isolate	1263:1279	the algal isolate	1263:1279	A combination of the genotypic and phenotypic data showed that the algal isolate represents a novel species of the genus Flavobacterium, for which the name Flavobacterium ahnfeltiae sp.
25002135	7	70	theme	genus	1104:1108	arg1	Flavobacterium					1110:1123	the genus Flavobacterium	1100:1123	the genus Flavobacterium	1100:1123	The new isolate and the type strains of recognized species of the genus Flavobacterium could strongly be distinguished by a number of phenotypic characteristics.
25002135	2	71	theme	Gram-negative	120:132	arg1	bacterium					195:203	A Gram-negative, aerobic, rod-shaped, motile by gliding and yellow-pigmented bacterium	118:203	A Gram-negative, aerobic, rod-shaped, motile by gliding and yellow-pigmented bacterium	118:203	A Gram-negative, aerobic, rod-shaped, motile by gliding and yellow-pigmented bacterium, designated strain 10Alg 130(T), that displayed the ability to destroy polysaccharides of red and brown algae, was isolated from the red alga Ahnfeltia tobuchiensis.
25002135	3	72	theme	sequence	565:572	arg1	similarities					574:585	sequence similarities	565:585	sequence similarities of 96.2 and 95.7 % to Flavobacterium jumunjiense KCTC 23618(T) and Flavobacterium ponti CCUG 58402(T), and 95.3-92.5 % to other recognized Flavobacterium species	565:747	The phylogenetic analysis based on 16S rRNA gene sequence placed the novel strain within the genus Flavobacterium, the type genus of the family Flavobacteriaceae, the phylum Bacteroidetes, with sequence similarities of 96.2 and 95.7 % to Flavobacterium jumunjiense KCTC 23618(T) and Flavobacterium ponti CCUG 58402(T), and 95.3-92.5 % to other recognized Flavobacterium species.
25002135	8	73	theme	ahnfeltiae	1371:1380	arg1	sp					1382:1383	the name Flavobacterium ahnfeltiae sp	1347:1383	the name Flavobacterium ahnfeltiae sp	1347:1383	A combination of the genotypic and phenotypic data showed that the algal isolate represents a novel species of the genus Flavobacterium, for which the name Flavobacterium ahnfeltiae sp.
25002135	3	74	theme	other	709:713	arg1	species					741:747	other recognized Flavobacterium species	709:747	other recognized Flavobacterium species	709:747	The phylogenetic analysis based on 16S rRNA gene sequence placed the novel strain within the genus Flavobacterium, the type genus of the family Flavobacteriaceae, the phylum Bacteroidetes, with sequence similarities of 96.2 and 95.7 % to Flavobacterium jumunjiense KCTC 23618(T) and Flavobacterium ponti CCUG 58402(T), and 95.3-92.5 % to other recognized Flavobacterium species.
27072676	6	0	theme	sporophyte	893:902	arg1	thalli					904:909	the sporophyte thalli	889:909	the sporophyte thalli	889:909	The lateral cell wall of vegetative cells of the sporophyte thalli had multilayered architecture containing electron-dense and negatively stained fibrils.
27072676	11	1	theme	cell	1905:1908	arg1	wall					1910:1913	the cell wall	1901:1913	the cell wall	1901:1913	It was concluded that the spatial arrangement of electron-dense fibrils was different between the layers and between the directions of the cell wall, and calcium was necessary for maintaining the fibrous layers in the cell wall.
27072676	7	2	theme	outer	1132:1136	arg1	layers					1138:1143	inner and outer layers	1122:1143	inner and outer layers	1122:1143	Electron tomographic analysis showed that the amount of the electron-dense fibrils and the junctions was different between inner and outer layers, and between the perpendicular and tangential directions of the cell wall.
27072676	1	3	theme	brown	269:273	arg1	wall					286:289	the brown algal cell wall	265:289	the brown algal cell wall	265:289	This work investigated a correlation between the three-dimensional architecture and compound-components of the brown algal cell wall.
27072676	6	4	theme	vegetative	869:878	arg1	cells					880:884	vegetative cells	869:884	vegetative cells of the sporophyte thalli	869:909	The lateral cell wall of vegetative cells of the sporophyte thalli had multilayered architecture containing electron-dense and negatively stained fibrils.
27072676	10	5	theme	alginate-calcium	1575:1590	arg1	gels					1600:1603	alginate-calcium fibrous gels	1575:1603	alginate-calcium fibrous gels	1575:1603	The results demonstrated that electron-dense fibrils were composed of alginate-calcium fibrous gels, and electron negatively stained fibrils were crystalline cellulose microfibrils.
27072676	5	6	theme	wall	610:613	arg1	architecture					585:596	the architecture	581:596	the architecture of the cell wall	581:613	Here, we investigated the architecture of the cell wall and the effect of extracellular calcium in the sporophyte and gametophyte of the model brown alga, Ectocarpus siliculosus (Dillwyn) Lyngbye, using transmission electron microscopy, histochemical, and immunohistochemical studies.
27072676	5	6	theme	wall	610:613	arg1	effect					623:628	the effect	619:628	the effect of extracellular calcium	619:653	Here, we investigated the architecture of the cell wall and the effect of extracellular calcium in the sporophyte and gametophyte of the model brown alga, Ectocarpus siliculosus (Dillwyn) Lyngbye, using transmission electron microscopy, histochemical, and immunohistochemical studies.
27072676	9	7	theme	integrity	1449:1457	arg1	Destruction					1424:1434	Destruction	1424:1434	Destruction of cell wall integrity	1424:1457	Destruction of cell wall integrity was also induced by the addition of sorbitol.
27072676	12	8	theme	wall	1995:1998	arg1	principle					1961:1969	the design principle	1950:1969	the design principle of the brown algal cell wall	1950:1998	This study provides insights into the design principle of the brown algal cell wall.
27072676	8	9	theme	low-calcium	1263:1273	arg1	seawater					1327:1334	the low-calcium (one-eighth of the normal concentration) artificial seawater	1259:1334	the low-calcium (one-eighth of the normal concentration) artificial seawater medium	1259:1341	By immersing the gametophyte thalli in the low-calcium (one-eighth of the normal concentration) artificial seawater medium, the fibrous layers of the lateral cell wall of vegetative cells became swollen.
27072676	11	10	theme	wall	1831:1834	arg1	directions					1808:1817	the directions	1804:1817	the directions of the cell wall	1804:1834	It was concluded that the spatial arrangement of electron-dense fibrils was different between the layers and between the directions of the cell wall, and calcium was necessary for maintaining the fibrous layers in the cell wall.
27072676	7	11	theme	junctions	1090:1098	arg1	amount					1045:1050	the amount	1041:1050	the amount of the electron-dense fibrils and the junctions	1041:1098	Electron tomographic analysis showed that the amount of the electron-dense fibrils and the junctions was different between inner and outer layers, and between the perpendicular and tangential directions of the cell wall.
27072676	7	11	theme	junctions	1090:1098	arg1	different					1104:1112	different	1104:1112	different	1104:1112	Electron tomographic analysis showed that the amount of the electron-dense fibrils and the junctions was different between inner and outer layers, and between the perpendicular and tangential directions of the cell wall.
27072676	7	11	theme	junctions	1090:1098	arg1	fibrils					1074:1080	the electron-dense fibrils	1055:1080	the electron-dense fibrils	1055:1080	Electron tomographic analysis showed that the amount of the electron-dense fibrils and the junctions was different between inner and outer layers, and between the perpendicular and tangential directions of the cell wall.
27072676	7	11	theme	junctions	1090:1098	arg1	junctions					1090:1098	the junctions	1086:1098	the junctions	1086:1098	Electron tomographic analysis showed that the amount of the electron-dense fibrils and the junctions was different between inner and outer layers, and between the perpendicular and tangential directions of the cell wall.
27072676	6	12	theme	electron-dense	952:965	arg1	fibrils					990:996	electron-dense and negatively stained fibrils	952:996	electron-dense and negatively stained fibrils	952:996	The lateral cell wall of vegetative cells of the sporophyte thalli had multilayered architecture containing electron-dense and negatively stained fibrils.
27072676	10	13	theme	crystalline	1651:1661	arg1	microfibrils					1673:1684	crystalline cellulose microfibrils	1651:1684	crystalline cellulose microfibrils	1651:1684	The results demonstrated that electron-dense fibrils were composed of alginate-calcium fibrous gels, and electron negatively stained fibrils were crystalline cellulose microfibrils.
27072676	5	14	theme	model	696:700	arg1	Ectocarpus					714:723	Ectocarpus	714:723	Ectocarpus	714:723	Here, we investigated the architecture of the cell wall and the effect of extracellular calcium in the sporophyte and gametophyte of the model brown alga, Ectocarpus siliculosus (Dillwyn) Lyngbye, using transmission electron microscopy, histochemical, and immunohistochemical studies.
27072676	5	14	theme	model	696:700	arg1	alga					708:711	the model brown alga	692:711	the model brown alga	692:711	Here, we investigated the architecture of the cell wall and the effect of extracellular calcium in the sporophyte and gametophyte of the model brown alga, Ectocarpus siliculosus (Dillwyn) Lyngbye, using transmission electron microscopy, histochemical, and immunohistochemical studies.
27072676	8	15	theme	gametophyte	1237:1247	arg1	thalli					1249:1254	the gametophyte thalli	1233:1254	the gametophyte thalli in the low-calcium (one-eighth of the normal concentration) artificial seawater medium	1233:1341	By immersing the gametophyte thalli in the low-calcium (one-eighth of the normal concentration) artificial seawater medium, the fibrous layers of the lateral cell wall of vegetative cells became swollen.
27072676	8	16	theme	concentration	1301:1313	arg1	one-eighth					1276:1285	one-eighth	1276:1285	one-eighth of the normal concentration	1276:1313	By immersing the gametophyte thalli in the low-calcium (one-eighth of the normal concentration) artificial seawater medium, the fibrous layers of the lateral cell wall of vegetative cells became swollen.
27072676	7	17	theme	electron-dense	1059:1072	arg1	fibrils					1074:1080	the electron-dense fibrils	1055:1080	the electron-dense fibrils	1055:1080	Electron tomographic analysis showed that the amount of the electron-dense fibrils and the junctions was different between inner and outer layers, and between the perpendicular and tangential directions of the cell wall.
27072676	9	18	theme	sorbitol	1495:1502	arg1	addition					1483:1490	the addition	1479:1490	the addition of sorbitol	1479:1502	Destruction of cell wall integrity was also induced by the addition of sorbitol.
27072676	8	19	theme	artificial	1316:1325	arg1	seawater					1327:1334	the low-calcium (one-eighth of the normal concentration) artificial seawater	1259:1334	the low-calcium (one-eighth of the normal concentration) artificial seawater medium	1259:1341	By immersing the gametophyte thalli in the low-calcium (one-eighth of the normal concentration) artificial seawater medium, the fibrous layers of the lateral cell wall of vegetative cells became swollen.
27072676	7	20	theme	Electron	999:1006	arg1	analysis					1020:1027	Electron tomographic analysis	999:1027	Electron tomographic analysis	999:1027	Electron tomographic analysis showed that the amount of the electron-dense fibrils and the junctions was different between inner and outer layers, and between the perpendicular and tangential directions of the cell wall.
27072676	0	21	theme	alginate	16:23	arg1	Distribution					0:11	Distribution	0:11	Distribution of alginate and cellulose	0:37	Distribution of alginate and cellulose and regulatory role of calcium in the cell wall of the brown alga Ectocarpus siliculosus (Ectocarpales, Phaeophyceae).
27072676	0	21	theme	alginate	16:23	arg1	role					54:57	regulatory role	43:57	regulatory role of calcium in the cell wall of the brown alga	43:103	Distribution of alginate and cellulose and regulatory role of calcium in the cell wall of the brown alga Ectocarpus siliculosus (Ectocarpales, Phaeophyceae).
27072676	0	22	dep	Ectocarpus	105:114	arg1	Distribution					0:11	Distribution	0:11	Distribution of alginate and cellulose	0:37	Distribution of alginate and cellulose and regulatory role of calcium in the cell wall of the brown alga Ectocarpus siliculosus (Ectocarpales, Phaeophyceae).
27072676	0	22	dep	Ectocarpus	105:114	arg1	siliculosus					116:126	Ectocarpus siliculosus (Ectocarpales, Phaeophyceae)	105:155	Ectocarpus siliculosus (Ectocarpales, Phaeophyceae)	105:155	Distribution of alginate and cellulose and regulatory role of calcium in the cell wall of the brown alga Ectocarpus siliculosus (Ectocarpales, Phaeophyceae).
27072676	0	22	dep	Ectocarpus	105:114	arg1	role					54:57	regulatory role	43:57	regulatory role of calcium in the cell wall of the brown alga	43:103	Distribution of alginate and cellulose and regulatory role of calcium in the cell wall of the brown alga Ectocarpus siliculosus (Ectocarpales, Phaeophyceae).
27072676	5	23	theme	calcium	647:653	arg1	architecture					585:596	the architecture	581:596	the architecture of the cell wall	581:613	Here, we investigated the architecture of the cell wall and the effect of extracellular calcium in the sporophyte and gametophyte of the model brown alga, Ectocarpus siliculosus (Dillwyn) Lyngbye, using transmission electron microscopy, histochemical, and immunohistochemical studies.
27072676	5	23	theme	calcium	647:653	arg1	effect					623:628	the effect	619:628	the effect of extracellular calcium	619:653	Here, we investigated the architecture of the cell wall and the effect of extracellular calcium in the sporophyte and gametophyte of the model brown alga, Ectocarpus siliculosus (Dillwyn) Lyngbye, using transmission electron microscopy, histochemical, and immunohistochemical studies.
27072676	0	24	from	Distribution	0:11	arg1	wall					82:85	the cell wall	73:85	the cell wall of the brown alga	73:103	Distribution of alginate and cellulose and regulatory role of calcium in the cell wall of the brown alga Ectocarpus siliculosus (Ectocarpales, Phaeophyceae).
27072676	0	25	theme	cell	77:80	arg1	wall					82:85	the cell wall	73:85	the cell wall of the brown alga	73:103	Distribution of alginate and cellulose and regulatory role of calcium in the cell wall of the brown alga Ectocarpus siliculosus (Ectocarpales, Phaeophyceae).
27072676	12	26	theme	design	1954:1959	arg1	principle					1961:1969	the design principle	1950:1969	the design principle of the brown algal cell wall	1950:1998	This study provides insights into the design principle of the brown algal cell wall.
27072676	9	27	theme	cell	1439:1442	arg1	integrity					1449:1457	cell wall integrity	1439:1457	cell wall integrity	1439:1457	Destruction of cell wall integrity was also induced by the addition of sorbitol.
27072676	3	28	theme	cell	374:377	arg1	wall					379:382	a unique cell wall	365:382	a unique cell wall consisting of alginate, cellulose, and sulfated polysaccharides	365:446	Brown algae have a unique cell wall consisting of alginate, cellulose, and sulfated polysaccharides.
27072676	8	29	theme	cell	1378:1381	arg1	wall					1383:1386	the lateral cell wall	1366:1386	the lateral cell wall of vegetative cells	1366:1406	By immersing the gametophyte thalli in the low-calcium (one-eighth of the normal concentration) artificial seawater medium, the fibrous layers of the lateral cell wall of vegetative cells became swollen.
27072676	1	30	theme	algal	275:279	arg1	wall					286:289	the brown algal cell wall	265:289	the brown algal cell wall	265:289	This work investigated a correlation between the three-dimensional architecture and compound-components of the brown algal cell wall.
27072676	7	31	theme	inner	1122:1126	arg1	layers					1138:1143	inner and outer layers	1122:1143	inner and outer layers	1122:1143	Electron tomographic analysis showed that the amount of the electron-dense fibrils and the junctions was different between inner and outer layers, and between the perpendicular and tangential directions of the cell wall.
27072676	7	32	theme	tangential	1180:1189	arg1	directions					1191:1200	the perpendicular and tangential directions	1158:1200	the perpendicular and tangential directions of the cell wall	1158:1217	Electron tomographic analysis showed that the amount of the electron-dense fibrils and the junctions was different between inner and outer layers, and between the perpendicular and tangential directions of the cell wall.
27072676	1	33	theme	wall	286:289	arg1	architecture					225:236	three-dimensional architecture	207:236	three-dimensional architecture	207:236	This work investigated a correlation between the three-dimensional architecture and compound-components of the brown algal cell wall.
27072676	1	33	theme	wall	286:289	arg1	compound-components					242:260	compound-components	242:260	compound-components	242:260	This work investigated a correlation between the three-dimensional architecture and compound-components of the brown algal cell wall.
27072676	6	34	theme	cell	856:859	arg1	wall					861:864	The lateral cell wall	844:864	The lateral cell wall of vegetative cells of the sporophyte thalli	844:909	The lateral cell wall of vegetative cells of the sporophyte thalli had multilayered architecture containing electron-dense and negatively stained fibrils.
27072676	11	35	theme	fibrils	1751:1757	arg1	different					1763:1771	different	1763:1771	different	1763:1771	It was concluded that the spatial arrangement of electron-dense fibrils was different between the layers and between the directions of the cell wall, and calcium was necessary for maintaining the fibrous layers in the cell wall.
27072676	11	35	theme	fibrils	1751:1757	arg1	arrangement					1721:1731	the spatial arrangement	1709:1731	the spatial arrangement of electron-dense fibrils	1709:1757	It was concluded that the spatial arrangement of electron-dense fibrils was different between the layers and between the directions of the cell wall, and calcium was necessary for maintaining the fibrous layers in the cell wall.
27072676	8	36	theme	cells	1402:1406	arg1	wall					1383:1386	the lateral cell wall	1366:1386	the lateral cell wall of vegetative cells	1366:1406	By immersing the gametophyte thalli in the low-calcium (one-eighth of the normal concentration) artificial seawater medium, the fibrous layers of the lateral cell wall of vegetative cells became swollen.
27072676	7	37	theme	perpendicular	1162:1174	arg1	directions					1191:1200	the perpendicular and tangential directions	1158:1200	the perpendicular and tangential directions of the cell wall	1158:1217	Electron tomographic analysis showed that the amount of the electron-dense fibrils and the junctions was different between inner and outer layers, and between the perpendicular and tangential directions of the cell wall.
27072676	0	38	theme	cellulose	29:37	arg1	Distribution					0:11	Distribution	0:11	Distribution of alginate and cellulose	0:37	Distribution of alginate and cellulose and regulatory role of calcium in the cell wall of the brown alga Ectocarpus siliculosus (Ectocarpales, Phaeophyceae).
27072676	0	38	theme	cellulose	29:37	arg1	role					54:57	regulatory role	43:57	regulatory role of calcium in the cell wall of the brown alga	43:103	Distribution of alginate and cellulose and regulatory role of calcium in the cell wall of the brown alga Ectocarpus siliculosus (Ectocarpales, Phaeophyceae).
27072676	10	39	theme	electron-dense	1535:1548	arg1	fibrils					1550:1556	electron-dense fibrils	1535:1556	electron-dense fibrils	1535:1556	The results demonstrated that electron-dense fibrils were composed of alginate-calcium fibrous gels, and electron negatively stained fibrils were crystalline cellulose microfibrils.
27072676	5	40	theme	transmission	762:773	arg1	microscopy					784:793	transmission electron microscopy	762:793	transmission electron microscopy	762:793	Here, we investigated the architecture of the cell wall and the effect of extracellular calcium in the sporophyte and gametophyte of the model brown alga, Ectocarpus siliculosus (Dillwyn) Lyngbye, using transmission electron microscopy, histochemical, and immunohistochemical studies.
27072676	8	41	dep	seawater	1327:1334	arg1	one-eighth					1276:1285	one-eighth	1276:1285	one-eighth of the normal concentration	1276:1313	By immersing the gametophyte thalli in the low-calcium (one-eighth of the normal concentration) artificial seawater medium, the fibrous layers of the lateral cell wall of vegetative cells became swollen.
27072676	3	42	contain	have	360:363	arg2	wall					379:382	a unique cell wall	365:382	a unique cell wall consisting of alginate, cellulose, and sulfated polysaccharides	365:446	Brown algae have a unique cell wall consisting of alginate, cellulose, and sulfated polysaccharides.
27072676	3	42	contain	have	360:363	arg1	algae					354:358	Brown algae	348:358	Brown algae	348:358	Brown algae have a unique cell wall consisting of alginate, cellulose, and sulfated polysaccharides.
27072676	4	43	theme	cell	527:530	arg1	wall					532:535	the cell wall	523:535	the cell wall	523:535	However, the relationship between the architecture and the composition of the cell wall is poorly understood.
27072676	6	44	contain	had	911:913	arg1	wall					861:864	The lateral cell wall	844:864	The lateral cell wall of vegetative cells of the sporophyte thalli	844:909	The lateral cell wall of vegetative cells of the sporophyte thalli had multilayered architecture containing electron-dense and negatively stained fibrils.
27072676	6	44	contain	had	911:913	arg2	architecture					928:939	multilayered architecture	915:939	multilayered architecture containing electron-dense and negatively stained fibrils	915:996	The lateral cell wall of vegetative cells of the sporophyte thalli had multilayered architecture containing electron-dense and negatively stained fibrils.
27072676	2	45	theme	cell	327:330	arg1	integrity					337:345	the cell wall integrity	323:345	the cell wall integrity	323:345	Calcium greatly contributes to the cell wall integrity.
27072676	6	46	theme	thalli	904:909	arg1	cells					880:884	vegetative cells	869:884	vegetative cells of the sporophyte thalli	869:909	The lateral cell wall of vegetative cells of the sporophyte thalli had multilayered architecture containing electron-dense and negatively stained fibrils.
27072676	5	47	theme	extracellular	633:645	arg1	calcium					647:653	extracellular calcium	633:653	extracellular calcium	633:653	Here, we investigated the architecture of the cell wall and the effect of extracellular calcium in the sporophyte and gametophyte of the model brown alga, Ectocarpus siliculosus (Dillwyn) Lyngbye, using transmission electron microscopy, histochemical, and immunohistochemical studies.
27072676	6	48	theme	cells	880:884	arg1	wall					861:864	The lateral cell wall	844:864	The lateral cell wall of vegetative cells of the sporophyte thalli	844:909	The lateral cell wall of vegetative cells of the sporophyte thalli had multilayered architecture containing electron-dense and negatively stained fibrils.
27072676	10	49	theme	fibrous	1592:1598	arg1	gels					1600:1603	alginate-calcium fibrous gels	1575:1603	alginate-calcium fibrous gels	1575:1603	The results demonstrated that electron-dense fibrils were composed of alginate-calcium fibrous gels, and electron negatively stained fibrils were crystalline cellulose microfibrils.
27072676	11	50	theme	spatial	1713:1719	arg1	different					1763:1771	different	1763:1771	different	1763:1771	It was concluded that the spatial arrangement of electron-dense fibrils was different between the layers and between the directions of the cell wall, and calcium was necessary for maintaining the fibrous layers in the cell wall.
27072676	11	50	theme	spatial	1713:1719	arg1	arrangement					1721:1731	the spatial arrangement	1709:1731	the spatial arrangement of electron-dense fibrils	1709:1757	It was concluded that the spatial arrangement of electron-dense fibrils was different between the layers and between the directions of the cell wall, and calcium was necessary for maintaining the fibrous layers in the cell wall.
27072676	5	51	theme	cell	605:608	arg1	wall					610:613	the cell wall	601:613	the cell wall	601:613	Here, we investigated the architecture of the cell wall and the effect of extracellular calcium in the sporophyte and gametophyte of the model brown alga, Ectocarpus siliculosus (Dillwyn) Lyngbye, using transmission electron microscopy, histochemical, and immunohistochemical studies.
27072676	9	52	theme	wall	1444:1447	arg1	integrity					1449:1457	cell wall integrity	1439:1457	cell wall integrity	1439:1457	Destruction of cell wall integrity was also induced by the addition of sorbitol.
27072676	0	53	theme	regulatory	43:52	arg1	role					54:57	regulatory role	43:57	regulatory role of calcium in the cell wall of the brown alga	43:103	Distribution of alginate and cellulose and regulatory role of calcium in the cell wall of the brown alga Ectocarpus siliculosus (Ectocarpales, Phaeophyceae).
27072676	10	54	theme	stained	1630:1636	arg1	fibrils					1638:1644	electron negatively stained fibrils	1610:1644	electron negatively stained fibrils	1610:1644	The results demonstrated that electron-dense fibrils were composed of alginate-calcium fibrous gels, and electron negatively stained fibrils were crystalline cellulose microfibrils.
27072676	12	55	theme	cell	1990:1993	arg1	wall					1995:1998	the brown algal cell wall	1974:1998	the brown algal cell wall	1974:1998	This study provides insights into the design principle of the brown algal cell wall.
27072676	6	56	theme	stained	982:988	arg1	fibrils					990:996	electron-dense and negatively stained fibrils	952:996	electron-dense and negatively stained fibrils	952:996	The lateral cell wall of vegetative cells of the sporophyte thalli had multilayered architecture containing electron-dense and negatively stained fibrils.
27072676	10	57	theme	electron	1610:1617	arg1	fibrils					1638:1644	electron negatively stained fibrils	1610:1644	electron negatively stained fibrils	1610:1644	The results demonstrated that electron-dense fibrils were composed of alginate-calcium fibrous gels, and electron negatively stained fibrils were crystalline cellulose microfibrils.
27072676	0	58	theme	alga	100:103	arg1	wall					82:85	the cell wall	73:85	the cell wall of the brown alga	73:103	Distribution of alginate and cellulose and regulatory role of calcium in the cell wall of the brown alga Ectocarpus siliculosus (Ectocarpales, Phaeophyceae).
27072676	1	59	dep	architecture	225:236	arg1	the					203:205	the	203:205	the	203:205	This work investigated a correlation between the three-dimensional architecture and compound-components of the brown algal cell wall.
27072676	10	60	theme	cellulose	1663:1671	arg1	microfibrils					1673:1684	crystalline cellulose microfibrils	1651:1684	crystalline cellulose microfibrils	1651:1684	The results demonstrated that electron-dense fibrils were composed of alginate-calcium fibrous gels, and electron negatively stained fibrils were crystalline cellulose microfibrils.
27072676	7	61	theme	fibrils	1074:1080	arg1	amount					1045:1050	the amount	1041:1050	the amount of the electron-dense fibrils and the junctions	1041:1098	Electron tomographic analysis showed that the amount of the electron-dense fibrils and the junctions was different between inner and outer layers, and between the perpendicular and tangential directions of the cell wall.
27072676	7	61	theme	fibrils	1074:1080	arg1	different					1104:1112	different	1104:1112	different	1104:1112	Electron tomographic analysis showed that the amount of the electron-dense fibrils and the junctions was different between inner and outer layers, and between the perpendicular and tangential directions of the cell wall.
27072676	7	61	theme	fibrils	1074:1080	arg1	fibrils					1074:1080	the electron-dense fibrils	1055:1080	the electron-dense fibrils	1055:1080	Electron tomographic analysis showed that the amount of the electron-dense fibrils and the junctions was different between inner and outer layers, and between the perpendicular and tangential directions of the cell wall.
27072676	7	61	theme	fibrils	1074:1080	arg1	junctions					1090:1098	the junctions	1086:1098	the junctions	1086:1098	Electron tomographic analysis showed that the amount of the electron-dense fibrils and the junctions was different between inner and outer layers, and between the perpendicular and tangential directions of the cell wall.
27072676	5	62	theme	immunohistochemical	815:833	arg1	studies					835:841	immunohistochemical studies	815:841	immunohistochemical studies	815:841	Here, we investigated the architecture of the cell wall and the effect of extracellular calcium in the sporophyte and gametophyte of the model brown alga, Ectocarpus siliculosus (Dillwyn) Lyngbye, using transmission electron microscopy, histochemical, and immunohistochemical studies.
27072676	7	63	theme	wall	1214:1217	arg1	directions					1191:1200	the perpendicular and tangential directions	1158:1200	the perpendicular and tangential directions of the cell wall	1158:1217	Electron tomographic analysis showed that the amount of the electron-dense fibrils and the junctions was different between inner and outer layers, and between the perpendicular and tangential directions of the cell wall.
27072676	12	64	theme	brown	1978:1982	arg1	wall					1995:1998	the brown algal cell wall	1974:1998	the brown algal cell wall	1974:1998	This study provides insights into the design principle of the brown algal cell wall.
27072676	6	65	theme	multilayered	915:926	arg1	architecture					928:939	multilayered architecture	915:939	multilayered architecture containing electron-dense and negatively stained fibrils	915:996	The lateral cell wall of vegetative cells of the sporophyte thalli had multilayered architecture containing electron-dense and negatively stained fibrils.
27072676	5	66	dep	Ectocarpus	714:723	arg1	siliculosus					725:735	Ectocarpus siliculosus	714:735	Ectocarpus siliculosus (Dillwyn) Lyngbye	714:753	Here, we investigated the architecture of the cell wall and the effect of extracellular calcium in the sporophyte and gametophyte of the model brown alga, Ectocarpus siliculosus (Dillwyn) Lyngbye, using transmission electron microscopy, histochemical, and immunohistochemical studies.
27072676	8	67	theme	normal	1294:1299	arg1	concentration					1301:1313	the normal concentration	1290:1313	the normal concentration	1290:1313	By immersing the gametophyte thalli in the low-calcium (one-eighth of the normal concentration) artificial seawater medium, the fibrous layers of the lateral cell wall of vegetative cells became swollen.
27072676	8	68	from	thalli	1249:1254	arg1	medium					1336:1341	the low-calcium (one-eighth of the normal concentration) artificial seawater medium	1259:1341	the low-calcium (one-eighth of the normal concentration) artificial seawater medium	1259:1341	By immersing the gametophyte thalli in the low-calcium (one-eighth of the normal concentration) artificial seawater medium, the fibrous layers of the lateral cell wall of vegetative cells became swollen.
27072676	0	69	theme	calcium	62:68	arg1	Distribution					0:11	Distribution	0:11	Distribution of alginate and cellulose	0:37	Distribution of alginate and cellulose and regulatory role of calcium in the cell wall of the brown alga Ectocarpus siliculosus (Ectocarpales, Phaeophyceae).
27072676	0	69	theme	calcium	62:68	arg1	role					54:57	regulatory role	43:57	regulatory role of calcium in the cell wall of the brown alga	43:103	Distribution of alginate and cellulose and regulatory role of calcium in the cell wall of the brown alga Ectocarpus siliculosus (Ectocarpales, Phaeophyceae).
27072676	7	70	theme	tomographic	1008:1018	arg1	analysis					1020:1027	Electron tomographic analysis	999:1027	Electron tomographic analysis	999:1027	Electron tomographic analysis showed that the amount of the electron-dense fibrils and the junctions was different between inner and outer layers, and between the perpendicular and tangential directions of the cell wall.
27072676	8	71	theme	seawater	1327:1334	arg1	medium					1336:1341	the low-calcium (one-eighth of the normal concentration) artificial seawater medium	1259:1341	the low-calcium (one-eighth of the normal concentration) artificial seawater medium	1259:1341	By immersing the gametophyte thalli in the low-calcium (one-eighth of the normal concentration) artificial seawater medium, the fibrous layers of the lateral cell wall of vegetative cells became swollen.
27072676	5	72	theme	alga	708:711	arg1	sporophyte					662:671	sporophyte	662:671	sporophyte	662:671	Here, we investigated the architecture of the cell wall and the effect of extracellular calcium in the sporophyte and gametophyte of the model brown alga, Ectocarpus siliculosus (Dillwyn) Lyngbye, using transmission electron microscopy, histochemical, and immunohistochemical studies.
27072676	5	72	theme	alga	708:711	arg1	gametophyte					677:687	gametophyte	677:687	gametophyte	677:687	Here, we investigated the architecture of the cell wall and the effect of extracellular calcium in the sporophyte and gametophyte of the model brown alga, Ectocarpus siliculosus (Dillwyn) Lyngbye, using transmission electron microscopy, histochemical, and immunohistochemical studies.
27072676	0	73	theme	brown	94:98	arg1	alga					100:103	the brown alga	90:103	the brown alga	90:103	Distribution of alginate and cellulose and regulatory role of calcium in the cell wall of the brown alga Ectocarpus siliculosus (Ectocarpales, Phaeophyceae).
27072676	11	74	theme	cell	1826:1829	arg1	wall					1831:1834	the cell wall	1822:1834	the cell wall	1822:1834	It was concluded that the spatial arrangement of electron-dense fibrils was different between the layers and between the directions of the cell wall, and calcium was necessary for maintaining the fibrous layers in the cell wall.
27072676	5	75	theme	brown	702:706	arg1	Ectocarpus					714:723	Ectocarpus	714:723	Ectocarpus	714:723	Here, we investigated the architecture of the cell wall and the effect of extracellular calcium in the sporophyte and gametophyte of the model brown alga, Ectocarpus siliculosus (Dillwyn) Lyngbye, using transmission electron microscopy, histochemical, and immunohistochemical studies.
27072676	5	75	theme	brown	702:706	arg1	alga					708:711	the model brown alga	692:711	the model brown alga	692:711	Here, we investigated the architecture of the cell wall and the effect of extracellular calcium in the sporophyte and gametophyte of the model brown alga, Ectocarpus siliculosus (Dillwyn) Lyngbye, using transmission electron microscopy, histochemical, and immunohistochemical studies.
27072676	0	76	from	role	54:57	arg1	wall					82:85	the cell wall	73:85	the cell wall of the brown alga	73:103	Distribution of alginate and cellulose and regulatory role of calcium in the cell wall of the brown alga Ectocarpus siliculosus (Ectocarpales, Phaeophyceae).
27072676	8	77	theme	fibrous	1348:1354	arg1	layers					1356:1361	the fibrous layers	1344:1361	the fibrous layers of the lateral cell wall of vegetative cells	1344:1406	By immersing the gametophyte thalli in the low-calcium (one-eighth of the normal concentration) artificial seawater medium, the fibrous layers of the lateral cell wall of vegetative cells became swollen.
27072676	3	78	theme	Brown	348:352	arg1	algae					354:358	Brown algae	348:358	Brown algae	348:358	Brown algae have a unique cell wall consisting of alginate, cellulose, and sulfated polysaccharides.
27072676	3	79	theme	sulfated	423:430	arg1	polysaccharides					432:446	sulfated polysaccharides	423:446	sulfated polysaccharides	423:446	Brown algae have a unique cell wall consisting of alginate, cellulose, and sulfated polysaccharides.
27072676	5	80	theme	electron	775:782	arg1	microscopy					784:793	transmission electron microscopy	762:793	transmission electron microscopy	762:793	Here, we investigated the architecture of the cell wall and the effect of extracellular calcium in the sporophyte and gametophyte of the model brown alga, Ectocarpus siliculosus (Dillwyn) Lyngbye, using transmission electron microscopy, histochemical, and immunohistochemical studies.
27072676	11	81	from	layers	1891:1896	arg1	wall					1910:1913	the cell wall	1901:1913	the cell wall	1901:1913	It was concluded that the spatial arrangement of electron-dense fibrils was different between the layers and between the directions of the cell wall, and calcium was necessary for maintaining the fibrous layers in the cell wall.
27072676	8	82	theme	lateral	1370:1376	arg1	wall					1383:1386	the lateral cell wall	1366:1386	the lateral cell wall of vegetative cells	1366:1406	By immersing the gametophyte thalli in the low-calcium (one-eighth of the normal concentration) artificial seawater medium, the fibrous layers of the lateral cell wall of vegetative cells became swollen.
27072676	1	83	theme	cell	281:284	arg1	wall					286:289	the brown algal cell wall	265:289	the brown algal cell wall	265:289	This work investigated a correlation between the three-dimensional architecture and compound-components of the brown algal cell wall.
27072676	3	84	theme	unique	367:372	arg1	wall					379:382	a unique cell wall	365:382	a unique cell wall consisting of alginate, cellulose, and sulfated polysaccharides	365:446	Brown algae have a unique cell wall consisting of alginate, cellulose, and sulfated polysaccharides.
27072676	11	85	theme	electron-dense	1736:1749	arg1	fibrils					1751:1757	electron-dense fibrils	1736:1757	electron-dense fibrils	1736:1757	It was concluded that the spatial arrangement of electron-dense fibrils was different between the layers and between the directions of the cell wall, and calcium was necessary for maintaining the fibrous layers in the cell wall.
27072676	8	86	theme	wall	1383:1386	arg1	layers					1356:1361	the fibrous layers	1344:1361	the fibrous layers of the lateral cell wall of vegetative cells	1344:1406	By immersing the gametophyte thalli in the low-calcium (one-eighth of the normal concentration) artificial seawater medium, the fibrous layers of the lateral cell wall of vegetative cells became swollen.
27072676	6	87	theme	lateral	848:854	arg1	wall					861:864	The lateral cell wall	844:864	The lateral cell wall of vegetative cells of the sporophyte thalli	844:909	The lateral cell wall of vegetative cells of the sporophyte thalli had multilayered architecture containing electron-dense and negatively stained fibrils.
27072676	8	88	theme	vegetative	1391:1400	arg1	cells					1402:1406	vegetative cells	1391:1406	vegetative cells	1391:1406	By immersing the gametophyte thalli in the low-calcium (one-eighth of the normal concentration) artificial seawater medium, the fibrous layers of the lateral cell wall of vegetative cells became swollen.
27072676	7	89	theme	cell	1209:1212	arg1	wall					1214:1217	the cell wall	1205:1217	the cell wall	1205:1217	Electron tomographic analysis showed that the amount of the electron-dense fibrils and the junctions was different between inner and outer layers, and between the perpendicular and tangential directions of the cell wall.
27072676	4	90	theme	wall	532:535	arg1	architecture					487:498	the architecture	483:498	the architecture	483:498	However, the relationship between the architecture and the composition of the cell wall is poorly understood.
27072676	4	90	theme	wall	532:535	arg1	composition					508:518	the composition	504:518	the composition	504:518	However, the relationship between the architecture and the composition of the cell wall is poorly understood.
27072676	2	91	theme	wall	332:335	arg1	integrity					337:345	the cell wall integrity	323:345	the cell wall integrity	323:345	Calcium greatly contributes to the cell wall integrity.
27072676	11	92	theme	fibrous	1883:1889	arg1	layers					1891:1896	the fibrous layers	1879:1896	the fibrous layers in the cell wall	1879:1913	It was concluded that the spatial arrangement of electron-dense fibrils was different between the layers and between the directions of the cell wall, and calcium was necessary for maintaining the fibrous layers in the cell wall.
27072676	1	93	theme	three-dimensional	207:223	arg1	architecture					225:236	three-dimensional architecture	207:236	three-dimensional architecture	207:236	This work investigated a correlation between the three-dimensional architecture and compound-components of the brown algal cell wall.
27072676	12	94	theme	algal	1984:1988	arg1	wall					1995:1998	the brown algal cell wall	1974:1998	the brown algal cell wall	1974:1998	This study provides insights into the design principle of the brown algal cell wall.
27072676	6	95	contain	containing	941:950	arg1	architecture					928:939	multilayered architecture	915:939	multilayered architecture containing electron-dense and negatively stained fibrils	915:996	The lateral cell wall of vegetative cells of the sporophyte thalli had multilayered architecture containing electron-dense and negatively stained fibrils.
27072676	6	95	contain	containing	941:950	arg2	fibrils					990:996	electron-dense and negatively stained fibrils	952:996	electron-dense and negatively stained fibrils	952:996	The lateral cell wall of vegetative cells of the sporophyte thalli had multilayered architecture containing electron-dense and negatively stained fibrils.
27771411	8	0	theme	monolayer	1221:1229	arg1	adsorption					1231:1240	Langmuir monolayer adsorption	1212:1240	Langmuir monolayer adsorption	1212:1240	The adsorption results showed that the arsenic for both arsenite [As(III)] and arsenate [As(V)] removal process followed a pseudo-second-order rate equation and Langmuir monolayer adsorption.
27771411	7	1	theme	FMBO	948:951	arg1	aerogels					963:970	Da-KGM/GO loaded FMBO composite aerogels	931:970	Da-KGM/GO loaded FMBO composite aerogels	931:970	Da-KGM/GO loaded FMBO composite aerogels exhibited high adsorption ability for arsenite [As(III)] and arsenate [As(V)].
27771411	2	2	theme	composite	326:334	arg1	matrix					336:341	composite matrix	326:341	composite matrix	326:341	Da-KGM, which was used as supporting composite matrix here, were firstly treated with GO and loaded FMBO.
27771411	9	3	theme	uptake	1273:1278	arg1	capacity					1280:1287	As(V) uptake capacity	1267:1287	As(V) uptake capacity	1267:1287	The maximum As(III) and As(V) uptake capacity of Da-KGM/GO(10%)/FMBO composite aerogels reached 30.21mgg-1 and 12.08mgg-1 respectively according to Langmuir isotherm at pH 7 and 323K.
27771411	0	4	theme	aqueous	81:87	arg1	solution					89:96	aqueous solution	81:96	aqueous solution	81:96	Da-KGM based GO-reinforced FMBO-loaded aerogels for efficient arsenic removal in aqueous solution.
27771411	6	5	theme	adsorption	866:875	arg1	tests					877:881	Batch adsorption tests	860:881	Batch adsorption tests	860:881	Batch adsorption tests were used to evaluate arsenic removal capacity.
27771411	8	6	dep	[As	1139:1141	arg1	V					1143:1143	V	1143:1143	V	1143:1143	The adsorption results showed that the arsenic for both arsenite [As(III)] and arsenate [As(V)] removal process followed a pseudo-second-order rate equation and Langmuir monolayer adsorption.
27771411	1	7	theme	graphene	164:171	arg1	oxide					173:177	graphene oxide	164:177	graphene oxide (GO) aerogels with iron and manganese oxides (FMBO) for effective removal of arsenic from contaminated water	164:286	Composites based on deacetylated konjac glucomannan (Da-KGM) and graphene oxide (GO) aerogels with iron and manganese oxides (FMBO) for effective removal of arsenic from contaminated water.
27771411	8	8	theme	arsenic	1090:1096	arg1	process					1155:1161	the arsenic for both arsenite [As(III)] and arsenate [As(V)] removal process	1086:1161	the arsenic for both arsenite [As(III)] and arsenate [As(V)] removal process	1086:1161	The adsorption results showed that the arsenic for both arsenite [As(III)] and arsenate [As(V)] removal process followed a pseudo-second-order rate equation and Langmuir monolayer adsorption.
27771411	6	9	theme	Batch	860:864	arg1	tests					877:881	Batch adsorption tests	860:881	Batch adsorption tests	860:881	Batch adsorption tests were used to evaluate arsenic removal capacity.
27771411	1	10	from	water	282:286	arg1	removal					245:251	effective removal	235:251	effective removal of arsenic from contaminated water	235:286	Composites based on deacetylated konjac glucomannan (Da-KGM) and graphene oxide (GO) aerogels with iron and manganese oxides (FMBO) for effective removal of arsenic from contaminated water.
27771411	4	11	theme	enhanced	693:700	arg1	properties					713:722	enhanced mechanical properties	693:722	enhanced mechanical properties	693:722	The characteristic results showed that addition of GO exhibited enhanced mechanical properties towards Da-KGM aerogels.
27771411	1	12	theme	effective	235:243	arg1	removal					245:251	effective removal	235:251	effective removal of arsenic from contaminated water	235:286	Composites based on deacetylated konjac glucomannan (Da-KGM) and graphene oxide (GO) aerogels with iron and manganese oxides (FMBO) for effective removal of arsenic from contaminated water.
27771411	9	13	theme	Langmuir	1391:1398	arg1	isotherm					1400:1407	Langmuir isotherm	1391:1407	Langmuir isotherm	1391:1407	The maximum As(III) and As(V) uptake capacity of Da-KGM/GO(10%)/FMBO composite aerogels reached 30.21mgg-1 and 12.08mgg-1 respectively according to Langmuir isotherm at pH 7 and 323K.
27771411	5	14	theme	strong	792:797	arg1	interaction					828:838	the strong intermolecular hydrogen bond interaction	788:838	the strong intermolecular hydrogen bond interaction between KGM and GO	788:857	What's more, results of FTIR indicated the strong intermolecular hydrogen bond interaction between KGM and GO.
27771411	4	15	theme	characteristic	633:646	arg1	results					648:654	The characteristic results	629:654	The characteristic results	629:654	The characteristic results showed that addition of GO exhibited enhanced mechanical properties towards Da-KGM aerogels.
27771411	3	16	theme	compression	464:474	arg1	fourier					542:548	fourier	542:548	fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM)	542:626	The obtained Da-KGM/GO/FMBO composite aerogels were characterized by compression test, thermo gravimetric analysis (TGA), X-ray diffraction (XRD), fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM).
27771411	3	16	theme	compression	464:474	arg1	diffraction					523:533	X-ray diffraction	517:533	X-ray diffraction (XRD)	517:539	The obtained Da-KGM/GO/FMBO composite aerogels were characterized by compression test, thermo gravimetric analysis (TGA), X-ray diffraction (XRD), fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM).
27771411	3	16	theme	compression	464:474	arg1	test					476:479	compression test	464:479	compression test	464:479	The obtained Da-KGM/GO/FMBO composite aerogels were characterized by compression test, thermo gravimetric analysis (TGA), X-ray diffraction (XRD), fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM).
27771411	3	16	theme	compression	464:474	arg1	analysis					501:508	thermo gravimetric analysis	482:508	thermo gravimetric analysis (TGA)	482:514	The obtained Da-KGM/GO/FMBO composite aerogels were characterized by compression test, thermo gravimetric analysis (TGA), X-ray diffraction (XRD), fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM).
27771411	1	17	with	glucomannan	139:149	arg1	FMBO					225:228	FMBO	225:228	FMBO	225:228	Composites based on deacetylated konjac glucomannan (Da-KGM) and graphene oxide (GO) aerogels with iron and manganese oxides (FMBO) for effective removal of arsenic from contaminated water.
27771411	1	17	with	glucomannan	139:149	arg1	oxides					217:222	iron and manganese oxides	198:222	oxides	217:222	Composites based on deacetylated konjac glucomannan (Da-KGM) and graphene oxide (GO) aerogels with iron and manganese oxides (FMBO) for effective removal of arsenic from contaminated water.
27771411	9	18	theme	composite	1312:1320	arg1	aerogels					1322:1329	Da-KGM/GO(10%)/FMBO composite aerogels	1292:1329	Da-KGM/GO(10%)/FMBO composite aerogels	1292:1329	The maximum As(III) and As(V) uptake capacity of Da-KGM/GO(10%)/FMBO composite aerogels reached 30.21mgg-1 and 12.08mgg-1 respectively according to Langmuir isotherm at pH 7 and 323K.
27771411	1	19	theme	oxide	173:177	arg1	aerogels					184:191	graphene oxide (GO) aerogels	164:191	graphene oxide (GO) aerogels with iron and manganese oxides (FMBO) for effective removal of arsenic from contaminated water	164:286	Composites based on deacetylated konjac glucomannan (Da-KGM) and graphene oxide (GO) aerogels with iron and manganese oxides (FMBO) for effective removal of arsenic from contaminated water.
27771411	9	20	theme	Da-KGM/GO	1292:1300	arg1	aerogels					1322:1329	Da-KGM/GO(10%)/FMBO composite aerogels	1292:1329	Da-KGM/GO(10%)/FMBO composite aerogels	1292:1329	The maximum As(III) and As(V) uptake capacity of Da-KGM/GO(10%)/FMBO composite aerogels reached 30.21mgg-1 and 12.08mgg-1 respectively according to Langmuir isotherm at pH 7 and 323K.
27771411	8	21	theme	pseudo-second-order	1174:1192	arg1	equation					1199:1206	a pseudo-second-order rate equation	1172:1206	a pseudo-second-order rate equation	1172:1206	The adsorption results showed that the arsenic for both arsenite [As(III)] and arsenate [As(V)] removal process followed a pseudo-second-order rate equation and Langmuir monolayer adsorption.
27771411	4	22	theme	Da-KGM	732:737	arg1	aerogels					739:746	Da-KGM aerogels	732:746	Da-KGM aerogels	732:746	The characteristic results showed that addition of GO exhibited enhanced mechanical properties towards Da-KGM aerogels.
27771411	3	23	theme	obtained	399:406	arg1	aerogels					433:440	The obtained Da-KGM/GO/FMBO composite aerogels	395:440	The obtained Da-KGM/GO/FMBO composite aerogels	395:440	The obtained Da-KGM/GO/FMBO composite aerogels were characterized by compression test, thermo gravimetric analysis (TGA), X-ray diffraction (XRD), fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM).
27771411	6	24	theme	removal	913:919	arg1	capacity					921:928	arsenic removal capacity	905:928	arsenic removal capacity	905:928	Batch adsorption tests were used to evaluate arsenic removal capacity.
27771411	7	25	theme	arsenate	1033:1040	arg1	[As					1042:1044	arsenate [As	1033:1044	arsenate [As(V)]	1033:1048	Da-KGM/GO loaded FMBO composite aerogels exhibited high adsorption ability for arsenite [As(III)] and arsenate [As(V)].
27771411	4	26	theme	mechanical	702:711	arg1	properties					713:722	enhanced mechanical properties	693:722	enhanced mechanical properties	693:722	The characteristic results showed that addition of GO exhibited enhanced mechanical properties towards Da-KGM aerogels.
27771411	3	27	dep	fourier	542:548	arg1	transform					550:558	transform	550:558	transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM)	550:626	The obtained Da-KGM/GO/FMBO composite aerogels were characterized by compression test, thermo gravimetric analysis (TGA), X-ray diffraction (XRD), fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM).
27771411	8	28	theme	arsenite	1107:1114	arg1	[As					1116:1118	arsenite [As	1107:1118	arsenite [As(III)]	1107:1124	The adsorption results showed that the arsenic for both arsenite [As(III)] and arsenate [As(V)] removal process followed a pseudo-second-order rate equation and Langmuir monolayer adsorption.
27771411	2	29	used	used	307:310	arg2	Da-KGM					289:294	Da-KGM	289:294	Da-KGM	289:294	Da-KGM, which was used as supporting composite matrix here, were firstly treated with GO and loaded FMBO.
27771411	4	30	theme	GO	680:681	arg1	addition					668:675	addition	668:675	addition of GO	668:681	The characteristic results showed that addition of GO exhibited enhanced mechanical properties towards Da-KGM aerogels.
27771411	0	31	theme	FMBO-loaded	27:37	arg1	aerogels					39:46	GO-reinforced FMBO-loaded aerogels	13:46	GO-reinforced FMBO-loaded aerogels	13:46	Da-KGM based GO-reinforced FMBO-loaded aerogels for efficient arsenic removal in aqueous solution.
27771411	1	32	theme	arsenic	256:262	arg1	removal					245:251	effective removal	235:251	effective removal of arsenic from contaminated water	235:286	Composites based on deacetylated konjac glucomannan (Da-KGM) and graphene oxide (GO) aerogels with iron and manganese oxides (FMBO) for effective removal of arsenic from contaminated water.
27771411	6	33	used	used	888:891	arg2	tests					877:881	Batch adsorption tests	860:881	Batch adsorption tests	860:881	Batch adsorption tests were used to evaluate arsenic removal capacity.
27771411	3	34	theme	X-ray	517:521	arg1	diffraction					523:533	X-ray diffraction	517:533	X-ray diffraction (XRD)	517:539	The obtained Da-KGM/GO/FMBO composite aerogels were characterized by compression test, thermo gravimetric analysis (TGA), X-ray diffraction (XRD), fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM).
27771411	3	34	theme	X-ray	517:521	arg1	test					476:479	compression test	464:479	compression test	464:479	The obtained Da-KGM/GO/FMBO composite aerogels were characterized by compression test, thermo gravimetric analysis (TGA), X-ray diffraction (XRD), fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM).
27771411	3	34	theme	X-ray	517:521	arg1	XRD					536:538	XRD	536:538	XRD	536:538	The obtained Da-KGM/GO/FMBO composite aerogels were characterized by compression test, thermo gravimetric analysis (TGA), X-ray diffraction (XRD), fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM).
27771411	8	35	theme	removal	1147:1153	arg1	process					1155:1161	the arsenic for both arsenite [As(III)] and arsenate [As(V)] removal process	1086:1161	the arsenic for both arsenite [As(III)] and arsenate [As(V)] removal process	1086:1161	The adsorption results showed that the arsenic for both arsenite [As(III)] and arsenate [As(V)] removal process followed a pseudo-second-order rate equation and Langmuir monolayer adsorption.
27771411	0	36	theme	GO-reinforced	13:25	arg1	aerogels					39:46	GO-reinforced FMBO-loaded aerogels	13:46	GO-reinforced FMBO-loaded aerogels	13:46	Da-KGM based GO-reinforced FMBO-loaded aerogels for efficient arsenic removal in aqueous solution.
27771411	9	37	theme	aerogels	1322:1329	arg1	III					1258:1260	III	1258:1260	III	1258:1260	The maximum As(III) and As(V) uptake capacity of Da-KGM/GO(10%)/FMBO composite aerogels reached 30.21mgg-1 and 12.08mgg-1 respectively according to Langmuir isotherm at pH 7 and 323K.
27771411	9	37	theme	aerogels	1322:1329	arg1	As					1255:1256	As	1255:1256	As	1255:1256	The maximum As(III) and As(V) uptake capacity of Da-KGM/GO(10%)/FMBO composite aerogels reached 30.21mgg-1 and 12.08mgg-1 respectively according to Langmuir isotherm at pH 7 and 323K.
27771411	3	38	theme	scanning	593:600	arg1	SEM					623:625	SEM	623:625	SEM	623:625	The obtained Da-KGM/GO/FMBO composite aerogels were characterized by compression test, thermo gravimetric analysis (TGA), X-ray diffraction (XRD), fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM).
27771411	3	38	theme	scanning	593:600	arg1	microscope					611:620	scanning electron microscope	593:620	scanning electron microscope (SEM)	593:626	The obtained Da-KGM/GO/FMBO composite aerogels were characterized by compression test, thermo gravimetric analysis (TGA), X-ray diffraction (XRD), fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM).
27771411	3	39	theme	thermo	482:487	arg1	test					476:479	compression test	464:479	compression test	464:479	The obtained Da-KGM/GO/FMBO composite aerogels were characterized by compression test, thermo gravimetric analysis (TGA), X-ray diffraction (XRD), fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM).
27771411	3	39	theme	thermo	482:487	arg1	TGA					511:513	TGA	511:513	TGA	511:513	The obtained Da-KGM/GO/FMBO composite aerogels were characterized by compression test, thermo gravimetric analysis (TGA), X-ray diffraction (XRD), fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM).
27771411	3	39	theme	thermo	482:487	arg1	analysis					501:508	thermo gravimetric analysis	482:508	thermo gravimetric analysis (TGA)	482:514	The obtained Da-KGM/GO/FMBO composite aerogels were characterized by compression test, thermo gravimetric analysis (TGA), X-ray diffraction (XRD), fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM).
27771411	8	40	theme	Langmuir	1212:1219	arg1	adsorption					1231:1240	Langmuir monolayer adsorption	1212:1240	Langmuir monolayer adsorption	1212:1240	The adsorption results showed that the arsenic for both arsenite [As(III)] and arsenate [As(V)] removal process followed a pseudo-second-order rate equation and Langmuir monolayer adsorption.
27771411	1	41	theme	contaminated	269:280	arg1	water					282:286	contaminated water	269:286	contaminated water	269:286	Composites based on deacetylated konjac glucomannan (Da-KGM) and graphene oxide (GO) aerogels with iron and manganese oxides (FMBO) for effective removal of arsenic from contaminated water.
27771411	1	42	with	aerogels	184:191	arg1	FMBO					225:228	FMBO	225:228	FMBO	225:228	Composites based on deacetylated konjac glucomannan (Da-KGM) and graphene oxide (GO) aerogels with iron and manganese oxides (FMBO) for effective removal of arsenic from contaminated water.
27771411	1	42	with	aerogels	184:191	arg1	oxides					217:222	iron and manganese oxides	198:222	oxides	217:222	Composites based on deacetylated konjac glucomannan (Da-KGM) and graphene oxide (GO) aerogels with iron and manganese oxides (FMBO) for effective removal of arsenic from contaminated water.
27771411	3	43	theme	electron	602:609	arg1	SEM					623:625	SEM	623:625	SEM	623:625	The obtained Da-KGM/GO/FMBO composite aerogels were characterized by compression test, thermo gravimetric analysis (TGA), X-ray diffraction (XRD), fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM).
27771411	3	43	theme	electron	602:609	arg1	microscope					611:620	scanning electron microscope	593:620	scanning electron microscope (SEM)	593:626	The obtained Da-KGM/GO/FMBO composite aerogels were characterized by compression test, thermo gravimetric analysis (TGA), X-ray diffraction (XRD), fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM).
27771411	1	44	theme	iron	198:201	arg1	FMBO					225:228	FMBO	225:228	FMBO	225:228	Composites based on deacetylated konjac glucomannan (Da-KGM) and graphene oxide (GO) aerogels with iron and manganese oxides (FMBO) for effective removal of arsenic from contaminated water.
27771411	1	44	theme	iron	198:201	arg1	oxides					217:222	iron and manganese oxides	198:222	oxides	217:222	Composites based on deacetylated konjac glucomannan (Da-KGM) and graphene oxide (GO) aerogels with iron and manganese oxides (FMBO) for effective removal of arsenic from contaminated water.
27771411	5	45	theme	hydrogen	814:821	arg1	interaction					828:838	the strong intermolecular hydrogen bond interaction	788:838	the strong intermolecular hydrogen bond interaction between KGM and GO	788:857	What's more, results of FTIR indicated the strong intermolecular hydrogen bond interaction between KGM and GO.
27771411	6	46	theme	arsenic	905:911	arg1	capacity					921:928	arsenic removal capacity	905:928	arsenic removal capacity	905:928	Batch adsorption tests were used to evaluate arsenic removal capacity.
27771411	7	47	dep	[As	1042:1044	arg1	V					1046:1046	V	1046:1046	V	1046:1046	Da-KGM/GO loaded FMBO composite aerogels exhibited high adsorption ability for arsenite [As(III)] and arsenate [As(V)].
27771411	7	48	theme	adsorption	987:996	arg1	ability					998:1004	high adsorption ability	982:1004	high adsorption ability	982:1004	Da-KGM/GO loaded FMBO composite aerogels exhibited high adsorption ability for arsenite [As(III)] and arsenate [As(V)].
27771411	3	49	theme	composite	423:431	arg1	aerogels					433:440	The obtained Da-KGM/GO/FMBO composite aerogels	395:440	The obtained Da-KGM/GO/FMBO composite aerogels	395:440	The obtained Da-KGM/GO/FMBO composite aerogels were characterized by compression test, thermo gravimetric analysis (TGA), X-ray diffraction (XRD), fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM).
27771411	0	50	theme	arsenic	62:68	arg1	removal					70:76	efficient arsenic removal	52:76	efficient arsenic removal in aqueous solution	52:96	Da-KGM based GO-reinforced FMBO-loaded aerogels for efficient arsenic removal in aqueous solution.
27771411	7	51	dep	[As	1019:1021	arg1	III					1023:1025	III	1023:1025	III	1023:1025	Da-KGM/GO loaded FMBO composite aerogels exhibited high adsorption ability for arsenite [As(III)] and arsenate [As(V)].
27771411	0	52	from	removal	70:76	arg1	solution					89:96	aqueous solution	81:96	aqueous solution	81:96	Da-KGM based GO-reinforced FMBO-loaded aerogels for efficient arsenic removal in aqueous solution.
27771411	1	53	dep	oxide	173:177	arg1	GO					180:181	GO	180:181	GO	180:181	Composites based on deacetylated konjac glucomannan (Da-KGM) and graphene oxide (GO) aerogels with iron and manganese oxides (FMBO) for effective removal of arsenic from contaminated water.
27771411	1	54	theme	deacetylated	119:130	arg1	glucomannan					139:149	deacetylated konjac glucomannan	119:149	deacetylated konjac glucomannan (Da-KGM)	119:158	Composites based on deacetylated konjac glucomannan (Da-KGM) and graphene oxide (GO) aerogels with iron and manganese oxides (FMBO) for effective removal of arsenic from contaminated water.
27771411	1	54	theme	deacetylated	119:130	arg1	Da-KGM					152:157	Da-KGM	152:157	Da-KGM	152:157	Composites based on deacetylated konjac glucomannan (Da-KGM) and graphene oxide (GO) aerogels with iron and manganese oxides (FMBO) for effective removal of arsenic from contaminated water.
27771411	0	55	theme	efficient	52:60	arg1	removal					70:76	efficient arsenic removal	52:76	efficient arsenic removal in aqueous solution	52:96	Da-KGM based GO-reinforced FMBO-loaded aerogels for efficient arsenic removal in aqueous solution.
27771411	1	56	theme	konjac	132:137	arg1	glucomannan					139:149	deacetylated konjac glucomannan	119:149	deacetylated konjac glucomannan (Da-KGM)	119:158	Composites based on deacetylated konjac glucomannan (Da-KGM) and graphene oxide (GO) aerogels with iron and manganese oxides (FMBO) for effective removal of arsenic from contaminated water.
27771411	1	56	theme	konjac	132:137	arg1	Da-KGM					152:157	Da-KGM	152:157	Da-KGM	152:157	Composites based on deacetylated konjac glucomannan (Da-KGM) and graphene oxide (GO) aerogels with iron and manganese oxides (FMBO) for effective removal of arsenic from contaminated water.
27771411	8	57	dep	[As	1116:1118	arg1	III					1120:1122	III	1120:1122	III	1120:1122	The adsorption results showed that the arsenic for both arsenite [As(III)] and arsenate [As(V)] removal process followed a pseudo-second-order rate equation and Langmuir monolayer adsorption.
27771411	9	58	dep	Da-KGM/GO	1292:1300	arg1	%					1304:1304	10%	1302:1304	10%	1302:1304	The maximum As(III) and As(V) uptake capacity of Da-KGM/GO(10%)/FMBO composite aerogels reached 30.21mgg-1 and 12.08mgg-1 respectively according to Langmuir isotherm at pH 7 and 323K.
27771411	7	59	theme	high	982:985	arg1	ability					998:1004	high adsorption ability	982:1004	high adsorption ability	982:1004	Da-KGM/GO loaded FMBO composite aerogels exhibited high adsorption ability for arsenite [As(III)] and arsenate [As(V)].
27771411	3	60	theme	infrared	560:567	arg1	spectroscopy					569:580	infrared spectroscopy	560:580	infrared spectroscopy (FTIR)	560:587	The obtained Da-KGM/GO/FMBO composite aerogels were characterized by compression test, thermo gravimetric analysis (TGA), X-ray diffraction (XRD), fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM).
27771411	3	60	theme	infrared	560:567	arg1	FTIR					583:586	FTIR	583:586	FTIR	583:586	The obtained Da-KGM/GO/FMBO composite aerogels were characterized by compression test, thermo gravimetric analysis (TGA), X-ray diffraction (XRD), fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM).
27771411	1	61	theme	manganese	207:215	arg1	FMBO					225:228	FMBO	225:228	FMBO	225:228	Composites based on deacetylated konjac glucomannan (Da-KGM) and graphene oxide (GO) aerogels with iron and manganese oxides (FMBO) for effective removal of arsenic from contaminated water.
27771411	1	61	theme	manganese	207:215	arg1	oxides					217:222	iron and manganese oxides	198:222	oxides	217:222	Composites based on deacetylated konjac glucomannan (Da-KGM) and graphene oxide (GO) aerogels with iron and manganese oxides (FMBO) for effective removal of arsenic from contaminated water.
27771411	7	62	theme	arsenite	1010:1017	arg1	[As					1019:1021	arsenite [As	1010:1021	arsenite [As(III)]	1010:1027	Da-KGM/GO loaded FMBO composite aerogels exhibited high adsorption ability for arsenite [As(III)] and arsenate [As(V)].
27771411	7	63	theme	composite	953:961	arg1	aerogels					963:970	Da-KGM/GO loaded FMBO composite aerogels	931:970	Da-KGM/GO loaded FMBO composite aerogels	931:970	Da-KGM/GO loaded FMBO composite aerogels exhibited high adsorption ability for arsenite [As(III)] and arsenate [As(V)].
27771411	8	64	theme	rate	1194:1197	arg1	equation					1199:1206	a pseudo-second-order rate equation	1172:1206	a pseudo-second-order rate equation	1172:1206	The adsorption results showed that the arsenic for both arsenite [As(III)] and arsenate [As(V)] removal process followed a pseudo-second-order rate equation and Langmuir monolayer adsorption.
27771411	8	65	theme	arsenate	1130:1137	arg1	[As					1139:1141	arsenate [As	1130:1141	arsenate [As(V)]	1130:1145	The adsorption results showed that the arsenic for both arsenite [As(III)] and arsenate [As(V)] removal process followed a pseudo-second-order rate equation and Langmuir monolayer adsorption.
27771411	5	66	theme	intermolecular	799:812	arg1	interaction					828:838	the strong intermolecular hydrogen bond interaction	788:838	the strong intermolecular hydrogen bond interaction between KGM and GO	788:857	What's more, results of FTIR indicated the strong intermolecular hydrogen bond interaction between KGM and GO.
27771411	3	67	theme	Da-KGM/GO/FMBO	408:421	arg1	aerogels					433:440	The obtained Da-KGM/GO/FMBO composite aerogels	395:440	The obtained Da-KGM/GO/FMBO composite aerogels	395:440	The obtained Da-KGM/GO/FMBO composite aerogels were characterized by compression test, thermo gravimetric analysis (TGA), X-ray diffraction (XRD), fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM).
27771411	9	68	theme	/FMBO	1306:1310	arg1	aerogels					1322:1329	Da-KGM/GO(10%)/FMBO composite aerogels	1292:1329	Da-KGM/GO(10%)/FMBO composite aerogels	1292:1329	The maximum As(III) and As(V) uptake capacity of Da-KGM/GO(10%)/FMBO composite aerogels reached 30.21mgg-1 and 12.08mgg-1 respectively according to Langmuir isotherm at pH 7 and 323K.
27771411	3	69	theme	gravimetric	489:499	arg1	test					476:479	compression test	464:479	compression test	464:479	The obtained Da-KGM/GO/FMBO composite aerogels were characterized by compression test, thermo gravimetric analysis (TGA), X-ray diffraction (XRD), fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM).
27771411	3	69	theme	gravimetric	489:499	arg1	TGA					511:513	TGA	511:513	TGA	511:513	The obtained Da-KGM/GO/FMBO composite aerogels were characterized by compression test, thermo gravimetric analysis (TGA), X-ray diffraction (XRD), fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM).
27771411	3	69	theme	gravimetric	489:499	arg1	analysis					501:508	thermo gravimetric analysis	482:508	thermo gravimetric analysis (TGA)	482:514	The obtained Da-KGM/GO/FMBO composite aerogels were characterized by compression test, thermo gravimetric analysis (TGA), X-ray diffraction (XRD), fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM).
27771411	5	70	theme	bond	823:826	arg1	interaction					828:838	the strong intermolecular hydrogen bond interaction	788:838	the strong intermolecular hydrogen bond interaction between KGM and GO	788:857	What's more, results of FTIR indicated the strong intermolecular hydrogen bond interaction between KGM and GO.
27771411	9	71	theme	V	1270:1270	arg1	capacity					1280:1287	As(V) uptake capacity	1267:1287	As(V) uptake capacity	1267:1287	The maximum As(III) and As(V) uptake capacity of Da-KGM/GO(10%)/FMBO composite aerogels reached 30.21mgg-1 and 12.08mgg-1 respectively according to Langmuir isotherm at pH 7 and 323K.
27771411	8	72	theme	adsorption	1055:1064	arg1	results					1066:1072	The adsorption results	1051:1072	The adsorption results	1051:1072	The adsorption results showed that the arsenic for both arsenite [As(III)] and arsenate [As(V)] removal process followed a pseudo-second-order rate equation and Langmuir monolayer adsorption.
27771411	5	73	theme	FTIR	773:776	arg1	results					762:768	results	762:768	results of FTIR	762:776	What's more, results of FTIR indicated the strong intermolecular hydrogen bond interaction between KGM and GO.
27771411	7	74	theme	loaded	941:946	arg1	aerogels					963:970	Da-KGM/GO loaded FMBO composite aerogels	931:970	Da-KGM/GO loaded FMBO composite aerogels	931:970	Da-KGM/GO loaded FMBO composite aerogels exhibited high adsorption ability for arsenite [As(III)] and arsenate [As(V)].
27771411	9	75	theme	As	1267:1268	arg1	capacity					1280:1287	As(V) uptake capacity	1267:1287	As(V) uptake capacity	1267:1287	The maximum As(III) and As(V) uptake capacity of Da-KGM/GO(10%)/FMBO composite aerogels reached 30.21mgg-1 and 12.08mgg-1 respectively according to Langmuir isotherm at pH 7 and 323K.
24737672	0	0	theme	Reticulum-Associated	77:96	arg1	Degradation					98:108	Endoplasmic Reticulum-Associated Degradation	65:108	Endoplasmic Reticulum-Associated Degradation of Misfolded Glycoproteins	65:135	Arabidopsis Class I α-Mannosidases MNS4 and MNS5 Are Involved in Endoplasmic Reticulum-Associated Degradation of Misfolded Glycoproteins.
24737672	6	1	theme	MNS4	1120:1123	arg1	Overexpression					1102:1115	Overexpression	1102:1115	Overexpression of MNS4 or MNS5 together with ER-retained glycoproteins	1102:1171	Overexpression of MNS4 or MNS5 together with ER-retained glycoproteins indicates further that both enzymes can convert Glc0-1Man8-9GlcNAc2 into N-glycans with a terminal α1,6-linked Man residue in the C-branch.
24737672	0	2	theme	Endoplasmic	65:75	arg1	Degradation					98:108	Endoplasmic Reticulum-Associated Degradation	65:108	Endoplasmic Reticulum-Associated Degradation of Misfolded Glycoproteins	65:135	Arabidopsis Class I α-Mannosidases MNS4 and MNS5 Are Involved in Endoplasmic Reticulum-Associated Degradation of Misfolded Glycoproteins.
24737672	7	3	theme	misfolded	1509:1517	arg1	glycoproteins					1519:1531	misfolded glycoproteins	1509:1531	misfolded glycoproteins	1509:1531	Thus, MNS4 and MNS5 function in the formation of unique N-glycan structures that are specifically recognized by other components of the ERAD machinery, which ultimately results in the disposal of misfolded glycoproteins.
24737672	7	4	theme	glycoproteins	1519:1531	arg1	disposal					1497:1504	the disposal	1493:1504	the disposal of misfolded glycoproteins	1493:1531	Thus, MNS4 and MNS5 function in the formation of unique N-glycan structures that are specifically recognized by other components of the ERAD machinery, which ultimately results in the disposal of misfolded glycoproteins.
24737672	5	5	theme	regular	1030:1036	arg1	processing					1047:1056	regular N-glycan processing	1030:1056	regular N-glycan processing of properly folded secretory glycoproteins	1030:1099	By contrast, N-glycan analysis of different mns mutant combinations revealed that MNS4 and MNS5 are not involved in regular N-glycan processing of properly folded secretory glycoproteins.
24737672	3	6	theme	Arabidopsis	544:554	arg1	thaliana					556:563	Arabidopsis thaliana class I α-mannosidases (MNS1 to MNS5)	544:601	Arabidopsis thaliana class I α-mannosidases (MNS1 to MNS5)	544:601	Here, we investigated the role of Arabidopsis thaliana class I α-mannosidases (MNS1 to MNS5) in glycan-dependent ERAD.
24737672	5	7	theme	mutant	962:967	arg1	combinations					969:980	different mns mutant combinations	948:980	different mns mutant combinations	948:980	By contrast, N-glycan analysis of different mns mutant combinations revealed that MNS4 and MNS5 are not involved in regular N-glycan processing of properly folded secretory glycoproteins.
24737672	4	8	theme	misfolded	750:758	arg1	variants					760:767	misfolded variants	750:767	misfolded variants of the heavily glycosylated brassinosteroid receptor, BRASSINOSTEROID INSENSITIVE1	750:850	Our genetic and biochemical data show that the two ER-resident proteins MNS4 and MNS5 are involved in the degradation of misfolded variants of the heavily glycosylated brassinosteroid receptor, BRASSINOSTEROID INSENSITIVE1, while MNS1 to MNS3 appear dispensable for this ERAD process.
24737672	4	9	dep	proteins	692:699	arg1	proteins					692:699	two ER-resident proteins	676:699	the two ER-resident proteins MNS4 and MNS5	672:713	Our genetic and biochemical data show that the two ER-resident proteins MNS4 and MNS5 are involved in the degradation of misfolded variants of the heavily glycosylated brassinosteroid receptor, BRASSINOSTEROID INSENSITIVE1, while MNS1 to MNS3 appear dispensable for this ERAD process.
24737672	4	9	dep	proteins	692:699	arg1	MNS5					710:713	MNS5	710:713	MNS5	710:713	Our genetic and biochemical data show that the two ER-resident proteins MNS4 and MNS5 are involved in the degradation of misfolded variants of the heavily glycosylated brassinosteroid receptor, BRASSINOSTEROID INSENSITIVE1, while MNS1 to MNS3 appear dispensable for this ERAD process.
24737672	4	9	dep	proteins	692:699	arg1	MNS4					701:704	MNS4	701:704	MNS4	701:704	Our genetic and biochemical data show that the two ER-resident proteins MNS4 and MNS5 are involved in the degradation of misfolded variants of the heavily glycosylated brassinosteroid receptor, BRASSINOSTEROID INSENSITIVE1, while MNS1 to MNS3 appear dispensable for this ERAD process.
24737672	3	10	theme	thaliana	556:563	arg1	role					536:539	the role	532:539	the role of Arabidopsis thaliana class I α-mannosidases (MNS1 to MNS5) in glycan-dependent ERAD	532:626	Here, we investigated the role of Arabidopsis thaliana class I α-mannosidases (MNS1 to MNS5) in glycan-dependent ERAD.
24737672	3	11	dep	thaliana	556:563	arg1	MNS5					597:600	MNS1 to MNS5	589:600	MNS1 to MNS5	589:600	Here, we investigated the role of Arabidopsis thaliana class I α-mannosidases (MNS1 to MNS5) in glycan-dependent ERAD.
24737672	3	11	dep	thaliana	556:563	arg1	α-mannosidases					573:586	class I α-mannosidases	565:586	Arabidopsis thaliana class I α-mannosidases (MNS1 to MNS5)	544:601	Here, we investigated the role of Arabidopsis thaliana class I α-mannosidases (MNS1 to MNS5) in glycan-dependent ERAD.
24737672	0	12	theme	Glycoproteins	123:135	arg1	Degradation					98:108	Endoplasmic Reticulum-Associated Degradation	65:108	Endoplasmic Reticulum-Associated Degradation of Misfolded Glycoproteins	65:135	Arabidopsis Class I α-Mannosidases MNS4 and MNS5 Are Involved in Endoplasmic Reticulum-Associated Degradation of Misfolded Glycoproteins.
24737672	7	13	theme	machinery	1454:1462	arg1	components					1431:1440	other components	1425:1440	other components of the ERAD machinery, which ultimately results in the disposal of misfolded glycoproteins	1425:1531	Thus, MNS4 and MNS5 function in the formation of unique N-glycan structures that are specifically recognized by other components of the ERAD machinery, which ultimately results in the disposal of misfolded glycoproteins.
24737672	3	14	dep	MNS5	597:600	arg1	to					594:595	to	594:595	to	594:595	Here, we investigated the role of Arabidopsis thaliana class I α-mannosidases (MNS1 to MNS5) in glycan-dependent ERAD.
24737672	7	15	theme	unique	1362:1367	arg1	structures					1378:1387	unique N-glycan structures	1362:1387	unique N-glycan structures that are specifically recognized by other components of the ERAD machinery, which ultimately results in the disposal of misfolded glycoproteins	1362:1531	Thus, MNS4 and MNS5 function in the formation of unique N-glycan structures that are specifically recognized by other components of the ERAD machinery, which ultimately results in the disposal of misfolded glycoproteins.
24737672	0	16	theme	Misfolded	113:121	arg1	Glycoproteins					123:135	Misfolded Glycoproteins	113:135	Misfolded Glycoproteins	113:135	Arabidopsis Class I α-Mannosidases MNS4 and MNS5 Are Involved in Endoplasmic Reticulum-Associated Degradation of Misfolded Glycoproteins.
24737672	2	17	from	degradation	487:497	arg1	plants					502:507	plants	502:507	plants	502:507	Many secretory proteins are N-glycosylated, and despite some recent progress, little is known about the mechanism that selects misfolded glycoproteins for degradation in plants.
24737672	6	18	gly	glycoproteins	1159:1171	arg1	glycoproteins					1159:1171	ER-retained glycoproteins	1147:1171	ER-retained glycoproteins	1147:1171	Overexpression of MNS4 or MNS5 together with ER-retained glycoproteins indicates further that both enzymes can convert Glc0-1Man8-9GlcNAc2 into N-glycans with a terminal α1,6-linked Man residue in the C-branch.
24737672	6	19	dep	indicates	1173:1181	arg1	convert					1213:1219	convert	1213:1219	can convert Glc0-1Man8-9GlcNAc2 into N-glycans with a terminal α1,6-linked Man residue in the C-branch	1209:1310	Overexpression of MNS4 or MNS5 together with ER-retained glycoproteins indicates further that both enzymes can convert Glc0-1Man8-9GlcNAc2 into N-glycans with a terminal α1,6-linked Man residue in the C-branch.
24737672	0	20	theme	Class	12:16	arg1	α-Mannosidases					20:33	Arabidopsis Class I α-Mannosidases MNS4 and MNS5	0:47	Arabidopsis Class I α-Mannosidases MNS4 and MNS5	0:47	Arabidopsis Class I α-Mannosidases MNS4 and MNS5 Are Involved in Endoplasmic Reticulum-Associated Degradation of Misfolded Glycoproteins.
24737672	0	20	theme	Class	12:16	arg1	MNS5					44:47	MNS5	44:47	MNS5	44:47	Arabidopsis Class I α-Mannosidases MNS4 and MNS5 Are Involved in Endoplasmic Reticulum-Associated Degradation of Misfolded Glycoproteins.
24737672	0	20	theme	Class	12:16	arg1	MNS4					35:38	MNS4	35:38	MNS4	35:38	Arabidopsis Class I α-Mannosidases MNS4 and MNS5 Are Involved in Endoplasmic Reticulum-Associated Degradation of Misfolded Glycoproteins.
24737672	7	21	theme	ERAD	1449:1452	arg1	machinery					1454:1462	the ERAD machinery	1445:1462	the ERAD machinery	1445:1462	Thus, MNS4 and MNS5 function in the formation of unique N-glycan structures that are specifically recognized by other components of the ERAD machinery, which ultimately results in the disposal of misfolded glycoproteins.
24737672	2	22	theme	recent	393:398	arg1	progress					400:407	some recent progress	388:407	some recent progress	388:407	Many secretory proteins are N-glycosylated, and despite some recent progress, little is known about the mechanism that selects misfolded glycoproteins for degradation in plants.
24737672	0	23	theme	Arabidopsis	0:10	arg1	α-Mannosidases					20:33	Arabidopsis Class I α-Mannosidases MNS4 and MNS5	0:47	Arabidopsis Class I α-Mannosidases MNS4 and MNS5	0:47	Arabidopsis Class I α-Mannosidases MNS4 and MNS5 Are Involved in Endoplasmic Reticulum-Associated Degradation of Misfolded Glycoproteins.
24737672	0	23	theme	Arabidopsis	0:10	arg1	MNS5					44:47	MNS5	44:47	MNS5	44:47	Arabidopsis Class I α-Mannosidases MNS4 and MNS5 Are Involved in Endoplasmic Reticulum-Associated Degradation of Misfolded Glycoproteins.
24737672	0	23	theme	Arabidopsis	0:10	arg1	MNS4					35:38	MNS4	35:38	MNS4	35:38	Arabidopsis Class I α-Mannosidases MNS4 and MNS5 Are Involved in Endoplasmic Reticulum-Associated Degradation of Misfolded Glycoproteins.
24737672	6	24	link	α1,6-linked	1272:1282	arg1	residue					1288:1294	a terminal α1,6-linked Man residue	1261:1294	a terminal α1,6-linked Man residue in the C-branch	1261:1310	Overexpression of MNS4 or MNS5 together with ER-retained glycoproteins indicates further that both enzymes can convert Glc0-1Man8-9GlcNAc2 into N-glycans with a terminal α1,6-linked Man residue in the C-branch.
24737672	5	25	theme	different	948:956	arg1	combinations					969:980	different mns mutant combinations	948:980	different mns mutant combinations	948:980	By contrast, N-glycan analysis of different mns mutant combinations revealed that MNS4 and MNS5 are not involved in regular N-glycan processing of properly folded secretory glycoproteins.
24737672	1	26	theme	endoplasmic	201:211	arg1	ER					224:225	ER	224:225	ER	224:225	To ensure that aberrantly folded proteins are cleared from the endoplasmic reticulum (ER), all eukaryotic cells possess a mechanism known as endoplasmic reticulum-associated degradation (ERAD).
24737672	1	26	theme	endoplasmic	201:211	arg1	reticulum					213:221	the endoplasmic reticulum	197:221	the endoplasmic reticulum (ER)	197:226	To ensure that aberrantly folded proteins are cleared from the endoplasmic reticulum (ER), all eukaryotic cells possess a mechanism known as endoplasmic reticulum-associated degradation (ERAD).
24737672	1	27	theme	endoplasmic	279:289	arg1	ERAD					325:328	ERAD	325:328	ERAD	325:328	To ensure that aberrantly folded proteins are cleared from the endoplasmic reticulum (ER), all eukaryotic cells possess a mechanism known as endoplasmic reticulum-associated degradation (ERAD).
24737672	1	27	theme	endoplasmic	279:289	arg1	degradation					312:322	endoplasmic reticulum-associated degradation	279:322	endoplasmic reticulum-associated degradation (ERAD)	279:329	To ensure that aberrantly folded proteins are cleared from the endoplasmic reticulum (ER), all eukaryotic cells possess a mechanism known as endoplasmic reticulum-associated degradation (ERAD).
24737672	5	28	theme	N-glycan	1038:1045	arg1	processing					1047:1056	regular N-glycan processing	1030:1056	regular N-glycan processing of properly folded secretory glycoproteins	1030:1099	By contrast, N-glycan analysis of different mns mutant combinations revealed that MNS4 and MNS5 are not involved in regular N-glycan processing of properly folded secretory glycoproteins.
24737672	3	29	theme	class	565:569	arg1	MNS5					597:600	MNS1 to MNS5	589:600	MNS1 to MNS5	589:600	Here, we investigated the role of Arabidopsis thaliana class I α-mannosidases (MNS1 to MNS5) in glycan-dependent ERAD.
24737672	3	29	theme	class	565:569	arg1	α-mannosidases					573:586	class I α-mannosidases	565:586	Arabidopsis thaliana class I α-mannosidases (MNS1 to MNS5)	544:601	Here, we investigated the role of Arabidopsis thaliana class I α-mannosidases (MNS1 to MNS5) in glycan-dependent ERAD.
24737672	4	30	theme	BRASSINOSTEROID	823:837	arg1	receptor					813:820	the heavily glycosylated brassinosteroid receptor	772:820	the heavily glycosylated brassinosteroid receptor	772:820	Our genetic and biochemical data show that the two ER-resident proteins MNS4 and MNS5 are involved in the degradation of misfolded variants of the heavily glycosylated brassinosteroid receptor, BRASSINOSTEROID INSENSITIVE1, while MNS1 to MNS3 appear dispensable for this ERAD process.
24737672	4	30	theme	BRASSINOSTEROID	823:837	arg1	INSENSITIVE1					839:850	BRASSINOSTEROID INSENSITIVE1	823:850	BRASSINOSTEROID INSENSITIVE1	823:850	Our genetic and biochemical data show that the two ER-resident proteins MNS4 and MNS5 are involved in the degradation of misfolded variants of the heavily glycosylated brassinosteroid receptor, BRASSINOSTEROID INSENSITIVE1, while MNS1 to MNS3 appear dispensable for this ERAD process.
24737672	4	31	theme	genetic	633:639	arg1	data					657:660	Our genetic and biochemical data	629:660	Our genetic and biochemical data	629:660	Our genetic and biochemical data show that the two ER-resident proteins MNS4 and MNS5 are involved in the degradation of misfolded variants of the heavily glycosylated brassinosteroid receptor, BRASSINOSTEROID INSENSITIVE1, while MNS1 to MNS3 appear dispensable for this ERAD process.
24737672	0	32	theme	I	18:18	arg1	α-Mannosidases					20:33	Arabidopsis Class I α-Mannosidases MNS4 and MNS5	0:47	Arabidopsis Class I α-Mannosidases MNS4 and MNS5	0:47	Arabidopsis Class I α-Mannosidases MNS4 and MNS5 Are Involved in Endoplasmic Reticulum-Associated Degradation of Misfolded Glycoproteins.
24737672	0	32	theme	I	18:18	arg1	MNS5					44:47	MNS5	44:47	MNS5	44:47	Arabidopsis Class I α-Mannosidases MNS4 and MNS5 Are Involved in Endoplasmic Reticulum-Associated Degradation of Misfolded Glycoproteins.
24737672	0	32	theme	I	18:18	arg1	MNS4					35:38	MNS4	35:38	MNS4	35:38	Arabidopsis Class I α-Mannosidases MNS4 and MNS5 Are Involved in Endoplasmic Reticulum-Associated Degradation of Misfolded Glycoproteins.
24737672	1	33	theme	reticulum-associated	291:310	arg1	ERAD					325:328	ERAD	325:328	ERAD	325:328	To ensure that aberrantly folded proteins are cleared from the endoplasmic reticulum (ER), all eukaryotic cells possess a mechanism known as endoplasmic reticulum-associated degradation (ERAD).
24737672	1	33	theme	reticulum-associated	291:310	arg1	degradation					312:322	endoplasmic reticulum-associated degradation	279:322	endoplasmic reticulum-associated degradation (ERAD)	279:329	To ensure that aberrantly folded proteins are cleared from the endoplasmic reticulum (ER), all eukaryotic cells possess a mechanism known as endoplasmic reticulum-associated degradation (ERAD).
24737672	5	34	theme	secretory	1077:1085	arg1	glycoproteins					1087:1099	properly folded secretory glycoproteins	1061:1099	properly folded secretory glycoproteins	1061:1099	By contrast, N-glycan analysis of different mns mutant combinations revealed that MNS4 and MNS5 are not involved in regular N-glycan processing of properly folded secretory glycoproteins.
24737672	3	35	theme	I	571:571	arg1	MNS5					597:600	MNS1 to MNS5	589:600	MNS1 to MNS5	589:600	Here, we investigated the role of Arabidopsis thaliana class I α-mannosidases (MNS1 to MNS5) in glycan-dependent ERAD.
24737672	3	35	theme	I	571:571	arg1	α-mannosidases					573:586	class I α-mannosidases	565:586	Arabidopsis thaliana class I α-mannosidases (MNS1 to MNS5)	544:601	Here, we investigated the role of Arabidopsis thaliana class I α-mannosidases (MNS1 to MNS5) in glycan-dependent ERAD.
24737672	7	36	gly	glycoproteins	1519:1531	arg1	glycoproteins					1519:1531	misfolded glycoproteins	1509:1531	misfolded glycoproteins	1509:1531	Thus, MNS4 and MNS5 function in the formation of unique N-glycan structures that are specifically recognized by other components of the ERAD machinery, which ultimately results in the disposal of misfolded glycoproteins.
24737672	6	37	theme	MNS5	1128:1131	arg1	Overexpression					1102:1115	Overexpression	1102:1115	Overexpression of MNS4 or MNS5 together with ER-retained glycoproteins	1102:1171	Overexpression of MNS4 or MNS5 together with ER-retained glycoproteins indicates further that both enzymes can convert Glc0-1Man8-9GlcNAc2 into N-glycans with a terminal α1,6-linked Man residue in the C-branch.
24737672	5	38	theme	combinations	969:980	arg1	analysis					936:943	N-glycan analysis	927:943	N-glycan analysis of different mns mutant combinations	927:980	By contrast, N-glycan analysis of different mns mutant combinations revealed that MNS4 and MNS5 are not involved in regular N-glycan processing of properly folded secretory glycoproteins.
24737672	2	39	gly	N-glycosylated	360:373	arg1	proteins					347:354	Many secretory proteins	332:354	Many secretory proteins	332:354	Many secretory proteins are N-glycosylated, and despite some recent progress, little is known about the mechanism that selects misfolded glycoproteins for degradation in plants.
24737672	5	40	theme	glycoproteins	1087:1099	arg1	processing					1047:1056	regular N-glycan processing	1030:1056	regular N-glycan processing of properly folded secretory glycoproteins	1030:1099	By contrast, N-glycan analysis of different mns mutant combinations revealed that MNS4 and MNS5 are not involved in regular N-glycan processing of properly folded secretory glycoproteins.
24737672	4	41	theme	brassinosteroid	797:811	arg1	receptor					813:820	the heavily glycosylated brassinosteroid receptor	772:820	the heavily glycosylated brassinosteroid receptor	772:820	Our genetic and biochemical data show that the two ER-resident proteins MNS4 and MNS5 are involved in the degradation of misfolded variants of the heavily glycosylated brassinosteroid receptor, BRASSINOSTEROID INSENSITIVE1, while MNS1 to MNS3 appear dispensable for this ERAD process.
24737672	4	41	theme	brassinosteroid	797:811	arg1	INSENSITIVE1					839:850	BRASSINOSTEROID INSENSITIVE1	823:850	BRASSINOSTEROID INSENSITIVE1	823:850	Our genetic and biochemical data show that the two ER-resident proteins MNS4 and MNS5 are involved in the degradation of misfolded variants of the heavily glycosylated brassinosteroid receptor, BRASSINOSTEROID INSENSITIVE1, while MNS1 to MNS3 appear dispensable for this ERAD process.
24737672	4	42	theme	biochemical	645:655	arg1	data					657:660	Our genetic and biochemical data	629:660	Our genetic and biochemical data	629:660	Our genetic and biochemical data show that the two ER-resident proteins MNS4 and MNS5 are involved in the degradation of misfolded variants of the heavily glycosylated brassinosteroid receptor, BRASSINOSTEROID INSENSITIVE1, while MNS1 to MNS3 appear dispensable for this ERAD process.
24737672	4	43	gly	glycosylated	784:795	arg1	receptor					813:820	the heavily glycosylated brassinosteroid receptor	772:820	the heavily glycosylated brassinosteroid receptor	772:820	Our genetic and biochemical data show that the two ER-resident proteins MNS4 and MNS5 are involved in the degradation of misfolded variants of the heavily glycosylated brassinosteroid receptor, BRASSINOSTEROID INSENSITIVE1, while MNS1 to MNS3 appear dispensable for this ERAD process.
24737672	4	43	gly	glycosylated	784:795	arg1	INSENSITIVE1					839:850	BRASSINOSTEROID INSENSITIVE1	823:850	BRASSINOSTEROID INSENSITIVE1	823:850	Our genetic and biochemical data show that the two ER-resident proteins MNS4 and MNS5 are involved in the degradation of misfolded variants of the heavily glycosylated brassinosteroid receptor, BRASSINOSTEROID INSENSITIVE1, while MNS1 to MNS3 appear dispensable for this ERAD process.
24737672	6	44	theme	Man	1284:1286	arg1	residue					1288:1294	a terminal α1,6-linked Man residue	1261:1294	a terminal α1,6-linked Man residue in the C-branch	1261:1310	Overexpression of MNS4 or MNS5 together with ER-retained glycoproteins indicates further that both enzymes can convert Glc0-1Man8-9GlcNAc2 into N-glycans with a terminal α1,6-linked Man residue in the C-branch.
24737672	7	45	theme	structures	1378:1387	arg1	formation					1349:1357	the formation	1345:1357	the formation of unique N-glycan structures that are specifically recognized by other components of the ERAD machinery, which ultimately results in the disposal of misfolded glycoproteins	1345:1531	Thus, MNS4 and MNS5 function in the formation of unique N-glycan structures that are specifically recognized by other components of the ERAD machinery, which ultimately results in the disposal of misfolded glycoproteins.
24737672	3	46	theme	MNS1	589:592	arg1	MNS5					597:600	MNS1 to MNS5	589:600	MNS1 to MNS5	589:600	Here, we investigated the role of Arabidopsis thaliana class I α-mannosidases (MNS1 to MNS5) in glycan-dependent ERAD.
24737672	3	46	theme	MNS1	589:592	arg1	α-mannosidases					573:586	class I α-mannosidases	565:586	Arabidopsis thaliana class I α-mannosidases (MNS1 to MNS5)	544:601	Here, we investigated the role of Arabidopsis thaliana class I α-mannosidases (MNS1 to MNS5) in glycan-dependent ERAD.
24737672	2	47	theme	misfolded	459:467	arg1	glycoproteins					469:481	misfolded glycoproteins	459:481	misfolded glycoproteins	459:481	Many secretory proteins are N-glycosylated, and despite some recent progress, little is known about the mechanism that selects misfolded glycoproteins for degradation in plants.
24737672	4	48	theme	ERAD	900:903	arg1	process					905:911	this ERAD process	895:911	this ERAD process	895:911	Our genetic and biochemical data show that the two ER-resident proteins MNS4 and MNS5 are involved in the degradation of misfolded variants of the heavily glycosylated brassinosteroid receptor, BRASSINOSTEROID INSENSITIVE1, while MNS1 to MNS3 appear dispensable for this ERAD process.
24737672	7	49	theme	other	1425:1429	arg1	components					1431:1440	other components	1425:1440	other components of the ERAD machinery, which ultimately results in the disposal of misfolded glycoproteins	1425:1531	Thus, MNS4 and MNS5 function in the formation of unique N-glycan structures that are specifically recognized by other components of the ERAD machinery, which ultimately results in the disposal of misfolded glycoproteins.
24737672	2	50	gly	glycoproteins	469:481	arg1	glycoproteins					469:481	misfolded glycoproteins	459:481	misfolded glycoproteins	459:481	Many secretory proteins are N-glycosylated, and despite some recent progress, little is known about the mechanism that selects misfolded glycoproteins for degradation in plants.
24737672	6	51	theme	ER-retained	1147:1157	arg1	glycoproteins					1159:1171	ER-retained glycoproteins	1147:1171	ER-retained glycoproteins	1147:1171	Overexpression of MNS4 or MNS5 together with ER-retained glycoproteins indicates further that both enzymes can convert Glc0-1Man8-9GlcNAc2 into N-glycans with a terminal α1,6-linked Man residue in the C-branch.
24737672	5	52	gly	glycoproteins	1087:1099	arg1	glycoproteins					1087:1099	properly folded secretory glycoproteins	1061:1099	properly folded secretory glycoproteins	1061:1099	By contrast, N-glycan analysis of different mns mutant combinations revealed that MNS4 and MNS5 are not involved in regular N-glycan processing of properly folded secretory glycoproteins.
24737672	3	53	theme	glycan-dependent	606:621	arg1	ERAD					623:626	glycan-dependent ERAD	606:626	glycan-dependent ERAD	606:626	Here, we investigated the role of Arabidopsis thaliana class I α-mannosidases (MNS1 to MNS5) in glycan-dependent ERAD.
24737672	4	54	theme	glycosylated	784:795	arg1	receptor					813:820	the heavily glycosylated brassinosteroid receptor	772:820	the heavily glycosylated brassinosteroid receptor	772:820	Our genetic and biochemical data show that the two ER-resident proteins MNS4 and MNS5 are involved in the degradation of misfolded variants of the heavily glycosylated brassinosteroid receptor, BRASSINOSTEROID INSENSITIVE1, while MNS1 to MNS3 appear dispensable for this ERAD process.
24737672	4	54	theme	glycosylated	784:795	arg1	INSENSITIVE1					839:850	BRASSINOSTEROID INSENSITIVE1	823:850	BRASSINOSTEROID INSENSITIVE1	823:850	Our genetic and biochemical data show that the two ER-resident proteins MNS4 and MNS5 are involved in the degradation of misfolded variants of the heavily glycosylated brassinosteroid receptor, BRASSINOSTEROID INSENSITIVE1, while MNS1 to MNS3 appear dispensable for this ERAD process.
24737672	0	55	dep	α-Mannosidases	20:33	arg1	α-Mannosidases					20:33	Arabidopsis Class I α-Mannosidases MNS4 and MNS5	0:47	Arabidopsis Class I α-Mannosidases MNS4 and MNS5	0:47	Arabidopsis Class I α-Mannosidases MNS4 and MNS5 Are Involved in Endoplasmic Reticulum-Associated Degradation of Misfolded Glycoproteins.
24737672	0	55	dep	α-Mannosidases	20:33	arg1	MNS5					44:47	MNS5	44:47	MNS5	44:47	Arabidopsis Class I α-Mannosidases MNS4 and MNS5 Are Involved in Endoplasmic Reticulum-Associated Degradation of Misfolded Glycoproteins.
24737672	0	55	dep	α-Mannosidases	20:33	arg1	MNS4					35:38	MNS4	35:38	MNS4	35:38	Arabidopsis Class I α-Mannosidases MNS4 and MNS5 Are Involved in Endoplasmic Reticulum-Associated Degradation of Misfolded Glycoproteins.
24737672	2	56	theme	secretory	337:345	arg1	proteins					347:354	Many secretory proteins	332:354	Many secretory proteins	332:354	Many secretory proteins are N-glycosylated, and despite some recent progress, little is known about the mechanism that selects misfolded glycoproteins for degradation in plants.
24737672	5	57	theme	mns	958:960	arg1	combinations					969:980	different mns mutant combinations	948:980	different mns mutant combinations	948:980	By contrast, N-glycan analysis of different mns mutant combinations revealed that MNS4 and MNS5 are not involved in regular N-glycan processing of properly folded secretory glycoproteins.
24737672	6	58	theme	α1,6-linked	1272:1282	arg1	residue					1288:1294	a terminal α1,6-linked Man residue	1261:1294	a terminal α1,6-linked Man residue in the C-branch	1261:1310	Overexpression of MNS4 or MNS5 together with ER-retained glycoproteins indicates further that both enzymes can convert Glc0-1Man8-9GlcNAc2 into N-glycans with a terminal α1,6-linked Man residue in the C-branch.
24737672	4	59	theme	variants	760:767	arg1	degradation					735:745	the degradation	731:745	the degradation of misfolded variants of the heavily glycosylated brassinosteroid receptor, BRASSINOSTEROID INSENSITIVE1	731:850	Our genetic and biochemical data show that the two ER-resident proteins MNS4 and MNS5 are involved in the degradation of misfolded variants of the heavily glycosylated brassinosteroid receptor, BRASSINOSTEROID INSENSITIVE1, while MNS1 to MNS3 appear dispensable for this ERAD process.
24737672	2	60	theme	Many	332:335	arg1	proteins					347:354	Many secretory proteins	332:354	Many secretory proteins	332:354	Many secretory proteins are N-glycosylated, and despite some recent progress, little is known about the mechanism that selects misfolded glycoproteins for degradation in plants.
24737672	6	61	from	residue	1288:1294	arg1	C-branch					1303:1310	the C-branch	1299:1310	the C-branch	1299:1310	Overexpression of MNS4 or MNS5 together with ER-retained glycoproteins indicates further that both enzymes can convert Glc0-1Man8-9GlcNAc2 into N-glycans with a terminal α1,6-linked Man residue in the C-branch.
24737672	5	62	theme	N-glycan	927:934	arg1	analysis					936:943	N-glycan analysis	927:943	N-glycan analysis of different mns mutant combinations	927:980	By contrast, N-glycan analysis of different mns mutant combinations revealed that MNS4 and MNS5 are not involved in regular N-glycan processing of properly folded secretory glycoproteins.
24737672	5	63	theme	folded	1070:1075	arg1	glycoproteins					1087:1099	properly folded secretory glycoproteins	1061:1099	properly folded secretory glycoproteins	1061:1099	By contrast, N-glycan analysis of different mns mutant combinations revealed that MNS4 and MNS5 are not involved in regular N-glycan processing of properly folded secretory glycoproteins.
24737672	4	64	theme	receptor	813:820	arg1	variants					760:767	misfolded variants	750:767	misfolded variants of the heavily glycosylated brassinosteroid receptor, BRASSINOSTEROID INSENSITIVE1	750:850	Our genetic and biochemical data show that the two ER-resident proteins MNS4 and MNS5 are involved in the degradation of misfolded variants of the heavily glycosylated brassinosteroid receptor, BRASSINOSTEROID INSENSITIVE1, while MNS1 to MNS3 appear dispensable for this ERAD process.
24737672	4	65	theme	ER-resident	680:690	arg1	proteins					692:699	two ER-resident proteins	676:699	the two ER-resident proteins MNS4 and MNS5	672:713	Our genetic and biochemical data show that the two ER-resident proteins MNS4 and MNS5 are involved in the degradation of misfolded variants of the heavily glycosylated brassinosteroid receptor, BRASSINOSTEROID INSENSITIVE1, while MNS1 to MNS3 appear dispensable for this ERAD process.
24737672	4	65	theme	ER-resident	680:690	arg1	MNS5					710:713	MNS5	710:713	MNS5	710:713	Our genetic and biochemical data show that the two ER-resident proteins MNS4 and MNS5 are involved in the degradation of misfolded variants of the heavily glycosylated brassinosteroid receptor, BRASSINOSTEROID INSENSITIVE1, while MNS1 to MNS3 appear dispensable for this ERAD process.
24737672	4	65	theme	ER-resident	680:690	arg1	MNS4					701:704	MNS4	701:704	MNS4	701:704	Our genetic and biochemical data show that the two ER-resident proteins MNS4 and MNS5 are involved in the degradation of misfolded variants of the heavily glycosylated brassinosteroid receptor, BRASSINOSTEROID INSENSITIVE1, while MNS1 to MNS3 appear dispensable for this ERAD process.
24737672	7	66	theme	N-glycan	1369:1376	arg1	structures					1378:1387	unique N-glycan structures	1362:1387	unique N-glycan structures that are specifically recognized by other components of the ERAD machinery, which ultimately results in the disposal of misfolded glycoproteins	1362:1531	Thus, MNS4 and MNS5 function in the formation of unique N-glycan structures that are specifically recognized by other components of the ERAD machinery, which ultimately results in the disposal of misfolded glycoproteins.
24737672	1	67	theme	eukaryotic	233:242	arg1	cells					244:248	all eukaryotic cells	229:248	all eukaryotic cells	229:248	To ensure that aberrantly folded proteins are cleared from the endoplasmic reticulum (ER), all eukaryotic cells possess a mechanism known as endoplasmic reticulum-associated degradation (ERAD).
24737672	1	68	contain	possess	250:256	arg1	cells					244:248	all eukaryotic cells	229:248	all eukaryotic cells	229:248	To ensure that aberrantly folded proteins are cleared from the endoplasmic reticulum (ER), all eukaryotic cells possess a mechanism known as endoplasmic reticulum-associated degradation (ERAD).
24737672	1	68	contain	possess	250:256	arg2	mechanism					260:268	a mechanism	258:268	a mechanism known as endoplasmic reticulum-associated degradation (ERAD)	258:329	To ensure that aberrantly folded proteins are cleared from the endoplasmic reticulum (ER), all eukaryotic cells possess a mechanism known as endoplasmic reticulum-associated degradation (ERAD).
24737672	6	69	theme	terminal	1263:1270	arg1	residue					1288:1294	a terminal α1,6-linked Man residue	1261:1294	a terminal α1,6-linked Man residue in the C-branch	1261:1310	Overexpression of MNS4 or MNS5 together with ER-retained glycoproteins indicates further that both enzymes can convert Glc0-1Man8-9GlcNAc2 into N-glycans with a terminal α1,6-linked Man residue in the C-branch.
24737672	1	70	theme	folded	164:169	arg1	proteins					171:178	aberrantly folded proteins	153:178	aberrantly folded proteins	153:178	To ensure that aberrantly folded proteins are cleared from the endoplasmic reticulum (ER), all eukaryotic cells possess a mechanism known as endoplasmic reticulum-associated degradation (ERAD).
24737672	3	71	from	role	536:539	arg1	ERAD					623:626	glycan-dependent ERAD	606:626	glycan-dependent ERAD	606:626	Here, we investigated the role of Arabidopsis thaliana class I α-mannosidases (MNS1 to MNS5) in glycan-dependent ERAD.
25965466	2	0	theme	optimal	498:504	arg1	value					506:510	an optimal value	495:510	an optimal value	495:510	Tensile strength (σf) values of initial and aged TPS100BC0.02GAx and (TPS100BC0.02GAx)75PLA25 specimens improved significantly to a maximal value as GA contents approached an optimal value, while their moisture content and elongation at break values reduced to a minimal value, respectively, as GA contents approached the optimal value.
25965466	4	1	theme	New	849:851	arg1	endotherms					861:870	New melting endotherms	849:870	New melting endotherms	849:870	New melting endotherms and diffraction peaks of VH-type starch crystals were found on DSC thermograms and WAXD patterns of aged TPS or TPS100BC0.02 specimens, respectively, while negligible retrogradation effect was found for most aged TPS100BC0.02GAx and/or (TPS100BC0.02GAx)75PLA25 specimens.
25965466	1	2	with	glutaraldehyde	242:255	arg1	molecules					274:282	starch molecules	267:282	starch molecules	267:282	Water proof and strength retention properties of thermoplastic starch (TPS) resins were successfully improved by reacting glutaraldehyde (GA) with starch molecules during their gelatinization processes.
25965466	1	3	theme	thermoplastic	169:181	arg1	resins					196:201	thermoplastic starch (TPS) resins	169:201	thermoplastic starch (TPS) resins	169:201	Water proof and strength retention properties of thermoplastic starch (TPS) resins were successfully improved by reacting glutaraldehyde (GA) with starch molecules during their gelatinization processes.
25965466	1	4	theme	starch	183:188	arg1	resins					196:201	thermoplastic starch (TPS) resins	169:201	thermoplastic starch (TPS) resins	169:201	Water proof and strength retention properties of thermoplastic starch (TPS) resins were successfully improved by reacting glutaraldehyde (GA) with starch molecules during their gelatinization processes.
25965466	2	5	from	values	566:571	arg1	elongation					546:555	elongation	546:555	elongation	546:555	Tensile strength (σf) values of initial and aged TPS100BC0.02GAx and (TPS100BC0.02GAx)75PLA25 specimens improved significantly to a maximal value as GA contents approached an optimal value, while their moisture content and elongation at break values reduced to a minimal value, respectively, as GA contents approached the optimal value.
25965466	2	5	from	values	566:571	arg1	content					534:540	their moisture content	519:540	their moisture content	519:540	Tensile strength (σf) values of initial and aged TPS100BC0.02GAx and (TPS100BC0.02GAx)75PLA25 specimens improved significantly to a maximal value as GA contents approached an optimal value, while their moisture content and elongation at break values reduced to a minimal value, respectively, as GA contents approached the optimal value.
25965466	2	6	theme	initial	355:361	arg1	TPS100BC0.02GAx					372:386	initial and aged TPS100BC0.02GAx	355:386	initial and aged TPS100BC0.02GAx	355:386	Tensile strength (σf) values of initial and aged TPS100BC0.02GAx and (TPS100BC0.02GAx)75PLA25 specimens improved significantly to a maximal value as GA contents approached an optimal value, while their moisture content and elongation at break values reduced to a minimal value, respectively, as GA contents approached the optimal value.
25965466	2	7	theme	optimal	645:651	arg1	value					653:657	the optimal value	641:657	the optimal value	641:657	Tensile strength (σf) values of initial and aged TPS100BC0.02GAx and (TPS100BC0.02GAx)75PLA25 specimens improved significantly to a maximal value as GA contents approached an optimal value, while their moisture content and elongation at break values reduced to a minimal value, respectively, as GA contents approached the optimal value.
25965466	4	8	theme	retrogradation	1039:1052	arg1	effect					1054:1059	negligible retrogradation effect	1028:1059	negligible retrogradation effect	1028:1059	New melting endotherms and diffraction peaks of VH-type starch crystals were found on DSC thermograms and WAXD patterns of aged TPS or TPS100BC0.02 specimens, respectively, while negligible retrogradation effect was found for most aged TPS100BC0.02GAx and/or (TPS100BC0.02GAx)75PLA25 specimens.
25965466	1	9	theme	starch	267:272	arg1	molecules					274:282	starch molecules	267:282	starch molecules	267:282	Water proof and strength retention properties of thermoplastic starch (TPS) resins were successfully improved by reacting glutaraldehyde (GA) with starch molecules during their gelatinization processes.
25965466	2	10	theme	moisture	525:532	arg1	content					534:540	their moisture content	519:540	their moisture content	519:540	Tensile strength (σf) values of initial and aged TPS100BC0.02GAx and (TPS100BC0.02GAx)75PLA25 specimens improved significantly to a maximal value as GA contents approached an optimal value, while their moisture content and elongation at break values reduced to a minimal value, respectively, as GA contents approached the optimal value.
25965466	3	11	theme	specimen	714:721	arg1	values					677:682	The σf retention values	660:682	The σf retention values of (TPS100BC0.02GA0.5)75PLA25 specimen aged for 56 days	660:738	The σf retention values of (TPS100BC0.02GA0.5)75PLA25 specimen aged for 56 days are more than 50 times higher than those of corresponding aged TPS and TPS100BC0.02 specimens, respectively.
25965466	3	11	theme	specimen	714:721	arg1	times					757:761	times	757:761	times	757:761	The σf retention values of (TPS100BC0.02GA0.5)75PLA25 specimen aged for 56 days are more than 50 times higher than those of corresponding aged TPS and TPS100BC0.02 specimens, respectively.
25965466	2	12	theme	minimal	586:592	arg1	value					594:598	a minimal value	584:598	a minimal value	584:598	Tensile strength (σf) values of initial and aged TPS100BC0.02GAx and (TPS100BC0.02GAx)75PLA25 specimens improved significantly to a maximal value as GA contents approached an optimal value, while their moisture content and elongation at break values reduced to a minimal value, respectively, as GA contents approached the optimal value.
25965466	4	13	theme	melting	853:859	arg1	endotherms					861:870	New melting endotherms	849:870	New melting endotherms	849:870	New melting endotherms and diffraction peaks of VH-type starch crystals were found on DSC thermograms and WAXD patterns of aged TPS or TPS100BC0.02 specimens, respectively, while negligible retrogradation effect was found for most aged TPS100BC0.02GAx and/or (TPS100BC0.02GAx)75PLA25 specimens.
25965466	4	14	theme	aged	972:975	arg1	TPS					977:979	aged TPS	972:979	aged TPS	972:979	New melting endotherms and diffraction peaks of VH-type starch crystals were found on DSC thermograms and WAXD patterns of aged TPS or TPS100BC0.02 specimens, respectively, while negligible retrogradation effect was found for most aged TPS100BC0.02GAx and/or (TPS100BC0.02GAx)75PLA25 specimens.
25965466	3	15	theme	aged	798:801	arg1	TPS					803:805	corresponding aged TPS	784:805	corresponding aged TPS	784:805	The σf retention values of (TPS100BC0.02GA0.5)75PLA25 specimen aged for 56 days are more than 50 times higher than those of corresponding aged TPS and TPS100BC0.02 specimens, respectively.
25965466	4	16	theme	specimens	997:1005	arg1	thermograms					939:949	DSC thermograms	935:949	DSC thermograms	935:949	New melting endotherms and diffraction peaks of VH-type starch crystals were found on DSC thermograms and WAXD patterns of aged TPS or TPS100BC0.02 specimens, respectively, while negligible retrogradation effect was found for most aged TPS100BC0.02GAx and/or (TPS100BC0.02GAx)75PLA25 specimens.
25965466	4	16	theme	specimens	997:1005	arg1	patterns					960:967	WAXD patterns	955:967	WAXD patterns	955:967	New melting endotherms and diffraction peaks of VH-type starch crystals were found on DSC thermograms and WAXD patterns of aged TPS or TPS100BC0.02 specimens, respectively, while negligible retrogradation effect was found for most aged TPS100BC0.02GAx and/or (TPS100BC0.02GAx)75PLA25 specimens.
25965466	3	17	theme	aged	723:726	arg1	values					677:682	The σf retention values	660:682	The σf retention values of (TPS100BC0.02GA0.5)75PLA25 specimen aged for 56 days	660:738	The σf retention values of (TPS100BC0.02GA0.5)75PLA25 specimen aged for 56 days are more than 50 times higher than those of corresponding aged TPS and TPS100BC0.02 specimens, respectively.
25965466	3	17	theme	aged	723:726	arg1	times					757:761	times	757:761	times	757:761	The σf retention values of (TPS100BC0.02GA0.5)75PLA25 specimen aged for 56 days are more than 50 times higher than those of corresponding aged TPS and TPS100BC0.02 specimens, respectively.
25965466	4	18	theme	TPS100BC0.02GAx	1109:1123	arg1	specimens					1133:1141	most aged TPS100BC0.02GAx and/or (TPS100BC0.02GAx)75PLA25 specimens	1075:1141	most aged TPS100BC0.02GAx and/or (TPS100BC0.02GAx)75PLA25 specimens	1075:1141	New melting endotherms and diffraction peaks of VH-type starch crystals were found on DSC thermograms and WAXD patterns of aged TPS or TPS100BC0.02 specimens, respectively, while negligible retrogradation effect was found for most aged TPS100BC0.02GAx and/or (TPS100BC0.02GAx)75PLA25 specimens.
25965466	3	19	theme	corresponding	784:796	arg1	TPS					803:805	corresponding aged TPS	784:805	corresponding aged TPS	784:805	The σf retention values of (TPS100BC0.02GA0.5)75PLA25 specimen aged for 56 days are more than 50 times higher than those of corresponding aged TPS and TPS100BC0.02 specimens, respectively.
25965466	4	20	theme	DSC	935:937	arg1	thermograms					939:949	DSC thermograms	935:949	DSC thermograms	935:949	New melting endotherms and diffraction peaks of VH-type starch crystals were found on DSC thermograms and WAXD patterns of aged TPS or TPS100BC0.02 specimens, respectively, while negligible retrogradation effect was found for most aged TPS100BC0.02GAx and/or (TPS100BC0.02GAx)75PLA25 specimens.
25965466	3	21	dep	times	757:761	arg1	higher					763:768	higher	763:768	higher	763:768	The σf retention values of (TPS100BC0.02GA0.5)75PLA25 specimen aged for 56 days are more than 50 times higher than those of corresponding aged TPS and TPS100BC0.02 specimens, respectively.
25965466	2	22	theme	GA	618:619	arg1	contents					621:628	GA contents	618:628	GA contents	618:628	Tensile strength (σf) values of initial and aged TPS100BC0.02GAx and (TPS100BC0.02GAx)75PLA25 specimens improved significantly to a maximal value as GA contents approached an optimal value, while their moisture content and elongation at break values reduced to a minimal value, respectively, as GA contents approached the optimal value.
25965466	2	23	theme	maximal	455:461	arg1	value					463:467	a maximal value	453:467	a maximal value	453:467	Tensile strength (σf) values of initial and aged TPS100BC0.02GAx and (TPS100BC0.02GAx)75PLA25 specimens improved significantly to a maximal value as GA contents approached an optimal value, while their moisture content and elongation at break values reduced to a minimal value, respectively, as GA contents approached the optimal value.
25965466	1	24	theme	TPS	191:193	arg1	resins					196:201	thermoplastic starch (TPS) resins	169:201	thermoplastic starch (TPS) resins	169:201	Water proof and strength retention properties of thermoplastic starch (TPS) resins were successfully improved by reacting glutaraldehyde (GA) with starch molecules during their gelatinization processes.
25965466	3	25	theme	75PLA25	706:712	arg1	specimen					714:721	(TPS100BC0.02GA0.5)75PLA25 specimen	687:721	(TPS100BC0.02GA0.5)75PLA25 specimen	687:721	The σf retention values of (TPS100BC0.02GA0.5)75PLA25 specimen aged for 56 days are more than 50 times higher than those of corresponding aged TPS and TPS100BC0.02 specimens, respectively.
25965466	4	26	theme	WAXD	955:958	arg1	patterns					960:967	WAXD patterns	955:967	WAXD patterns	955:967	New melting endotherms and diffraction peaks of VH-type starch crystals were found on DSC thermograms and WAXD patterns of aged TPS or TPS100BC0.02 specimens, respectively, while negligible retrogradation effect was found for most aged TPS100BC0.02GAx and/or (TPS100BC0.02GAx)75PLA25 specimens.
25965466	0	27	theme	Water	0:4	arg1	proof					6:10	Water proof	0:10	Water proof	0:10	Water proof and strength retention properties of thermoplastic starch based biocomposites modified with glutaraldehyde.
25965466	4	28	theme	TPS100BC0.02	984:995	arg1	specimens					997:1005	TPS100BC0.02 specimens	984:1005	TPS100BC0.02 specimens	984:1005	New melting endotherms and diffraction peaks of VH-type starch crystals were found on DSC thermograms and WAXD patterns of aged TPS or TPS100BC0.02 specimens, respectively, while negligible retrogradation effect was found for most aged TPS100BC0.02GAx and/or (TPS100BC0.02GAx)75PLA25 specimens.
25965466	0	29	dep	biocomposites	76:88	arg1	modified					90:97	modified	90:97	biocomposites modified with glutaraldehyde	76:117	Water proof and strength retention properties of thermoplastic starch based biocomposites modified with glutaraldehyde.
25965466	4	30	theme	TPS100BC0.02GAx	1085:1099	arg1	specimens					1133:1141	most aged TPS100BC0.02GAx and/or (TPS100BC0.02GAx)75PLA25 specimens	1075:1141	most aged TPS100BC0.02GAx and/or (TPS100BC0.02GAx)75PLA25 specimens	1075:1141	New melting endotherms and diffraction peaks of VH-type starch crystals were found on DSC thermograms and WAXD patterns of aged TPS or TPS100BC0.02 specimens, respectively, while negligible retrogradation effect was found for most aged TPS100BC0.02GAx and/or (TPS100BC0.02GAx)75PLA25 specimens.
25965466	1	31	theme	resins	196:201	arg1	properties					155:164	Water proof and strength retention properties	120:164	Water proof and strength retention properties of thermoplastic starch (TPS) resins	120:201	Water proof and strength retention properties of thermoplastic starch (TPS) resins were successfully improved by reacting glutaraldehyde (GA) with starch molecules during their gelatinization processes.
25965466	0	32	theme	strength	16:23	arg1	properties					35:44	strength retention properties	16:44	strength retention properties	16:44	Water proof and strength retention properties of thermoplastic starch based biocomposites modified with glutaraldehyde.
25965466	2	33	theme	break	560:564	arg1	values					566:571	break values	560:571	break values	560:571	Tensile strength (σf) values of initial and aged TPS100BC0.02GAx and (TPS100BC0.02GAx)75PLA25 specimens improved significantly to a maximal value as GA contents approached an optimal value, while their moisture content and elongation at break values reduced to a minimal value, respectively, as GA contents approached the optimal value.
25965466	2	34	theme	Tensile	323:329	arg1	σf					341:342	σf	341:342	σf	341:342	Tensile strength (σf) values of initial and aged TPS100BC0.02GAx and (TPS100BC0.02GAx)75PLA25 specimens improved significantly to a maximal value as GA contents approached an optimal value, while their moisture content and elongation at break values reduced to a minimal value, respectively, as GA contents approached the optimal value.
25965466	2	34	theme	Tensile	323:329	arg1	strength					331:338	Tensile strength	323:338	Tensile strength (σf) values of initial and aged TPS100BC0.02GAx and (TPS100BC0.02GAx)75PLA25 specimens	323:425	Tensile strength (σf) values of initial and aged TPS100BC0.02GAx and (TPS100BC0.02GAx)75PLA25 specimens improved significantly to a maximal value as GA contents approached an optimal value, while their moisture content and elongation at break values reduced to a minimal value, respectively, as GA contents approached the optimal value.
25965466	4	35	theme	aged	1080:1083	arg1	specimens					1133:1141	most aged TPS100BC0.02GAx and/or (TPS100BC0.02GAx)75PLA25 specimens	1075:1141	most aged TPS100BC0.02GAx and/or (TPS100BC0.02GAx)75PLA25 specimens	1075:1141	New melting endotherms and diffraction peaks of VH-type starch crystals were found on DSC thermograms and WAXD patterns of aged TPS or TPS100BC0.02 specimens, respectively, while negligible retrogradation effect was found for most aged TPS100BC0.02GAx and/or (TPS100BC0.02GAx)75PLA25 specimens.
25965466	2	36	theme	strength	331:338	arg1	values					345:350	Tensile strength (σf) values	323:350	Tensile strength (σf) values of initial and aged TPS100BC0.02GAx and (TPS100BC0.02GAx)75PLA25 specimens	323:425	Tensile strength (σf) values of initial and aged TPS100BC0.02GAx and (TPS100BC0.02GAx)75PLA25 specimens improved significantly to a maximal value as GA contents approached an optimal value, while their moisture content and elongation at break values reduced to a minimal value, respectively, as GA contents approached the optimal value.
25965466	1	37	theme	gelatinization	297:310	arg1	processes					312:320	their gelatinization processes	291:320	their gelatinization processes	291:320	Water proof and strength retention properties of thermoplastic starch (TPS) resins were successfully improved by reacting glutaraldehyde (GA) with starch molecules during their gelatinization processes.
25965466	4	38	theme	VH-type	897:903	arg1	crystals					912:919	VH-type starch crystals	897:919	VH-type starch crystals	897:919	New melting endotherms and diffraction peaks of VH-type starch crystals were found on DSC thermograms and WAXD patterns of aged TPS or TPS100BC0.02 specimens, respectively, while negligible retrogradation effect was found for most aged TPS100BC0.02GAx and/or (TPS100BC0.02GAx)75PLA25 specimens.
25965466	1	39	theme	Water	120:124	arg1	proof					126:130	Water proof	120:130	Water proof	120:130	Water proof and strength retention properties of thermoplastic starch (TPS) resins were successfully improved by reacting glutaraldehyde (GA) with starch molecules during their gelatinization processes.
25965466	4	40	theme	TPS	977:979	arg1	thermograms					939:949	DSC thermograms	935:949	DSC thermograms	935:949	New melting endotherms and diffraction peaks of VH-type starch crystals were found on DSC thermograms and WAXD patterns of aged TPS or TPS100BC0.02 specimens, respectively, while negligible retrogradation effect was found for most aged TPS100BC0.02GAx and/or (TPS100BC0.02GAx)75PLA25 specimens.
25965466	4	40	theme	TPS	977:979	arg1	patterns					960:967	WAXD patterns	955:967	WAXD patterns	955:967	New melting endotherms and diffraction peaks of VH-type starch crystals were found on DSC thermograms and WAXD patterns of aged TPS or TPS100BC0.02 specimens, respectively, while negligible retrogradation effect was found for most aged TPS100BC0.02GAx and/or (TPS100BC0.02GAx)75PLA25 specimens.
25965466	2	41	theme	specimens	417:425	arg1	values					345:350	Tensile strength (σf) values	323:350	Tensile strength (σf) values of initial and aged TPS100BC0.02GAx and (TPS100BC0.02GAx)75PLA25 specimens	323:425	Tensile strength (σf) values of initial and aged TPS100BC0.02GAx and (TPS100BC0.02GAx)75PLA25 specimens improved significantly to a maximal value as GA contents approached an optimal value, while their moisture content and elongation at break values reduced to a minimal value, respectively, as GA contents approached the optimal value.
25965466	0	42	theme	retention	25:33	arg1	properties					35:44	strength retention properties	16:44	strength retention properties	16:44	Water proof and strength retention properties of thermoplastic starch based biocomposites modified with glutaraldehyde.
25965466	3	43	theme	50	754:755	arg1	values					677:682	The σf retention values	660:682	The σf retention values of (TPS100BC0.02GA0.5)75PLA25 specimen aged for 56 days	660:738	The σf retention values of (TPS100BC0.02GA0.5)75PLA25 specimen aged for 56 days are more than 50 times higher than those of corresponding aged TPS and TPS100BC0.02 specimens, respectively.
25965466	3	43	theme	50	754:755	arg1	times					757:761	times	757:761	times	757:761	The σf retention values of (TPS100BC0.02GA0.5)75PLA25 specimen aged for 56 days are more than 50 times higher than those of corresponding aged TPS and TPS100BC0.02 specimens, respectively.
25965466	4	44	theme	crystals	912:919	arg1	peaks					888:892	diffraction peaks	876:892	diffraction peaks	876:892	New melting endotherms and diffraction peaks of VH-type starch crystals were found on DSC thermograms and WAXD patterns of aged TPS or TPS100BC0.02 specimens, respectively, while negligible retrogradation effect was found for most aged TPS100BC0.02GAx and/or (TPS100BC0.02GAx)75PLA25 specimens.
25965466	4	44	theme	crystals	912:919	arg1	endotherms					861:870	New melting endotherms	849:870	New melting endotherms	849:870	New melting endotherms and diffraction peaks of VH-type starch crystals were found on DSC thermograms and WAXD patterns of aged TPS or TPS100BC0.02 specimens, respectively, while negligible retrogradation effect was found for most aged TPS100BC0.02GAx and/or (TPS100BC0.02GAx)75PLA25 specimens.
25965466	2	45	theme	75PLA25	409:415	arg1	specimens					417:425	(TPS100BC0.02GAx)75PLA25 specimens	392:425	(TPS100BC0.02GAx)75PLA25 specimens	392:425	Tensile strength (σf) values of initial and aged TPS100BC0.02GAx and (TPS100BC0.02GAx)75PLA25 specimens improved significantly to a maximal value as GA contents approached an optimal value, while their moisture content and elongation at break values reduced to a minimal value, respectively, as GA contents approached the optimal value.
25965466	2	46	theme	GA	472:473	arg1	contents					475:482	GA contents	472:482	GA contents	472:482	Tensile strength (σf) values of initial and aged TPS100BC0.02GAx and (TPS100BC0.02GAx)75PLA25 specimens improved significantly to a maximal value as GA contents approached an optimal value, while their moisture content and elongation at break values reduced to a minimal value, respectively, as GA contents approached the optimal value.
25965466	4	47	theme	diffraction	876:886	arg1	peaks					888:892	diffraction peaks	876:892	diffraction peaks	876:892	New melting endotherms and diffraction peaks of VH-type starch crystals were found on DSC thermograms and WAXD patterns of aged TPS or TPS100BC0.02 specimens, respectively, while negligible retrogradation effect was found for most aged TPS100BC0.02GAx and/or (TPS100BC0.02GAx)75PLA25 specimens.
25965466	0	48	theme	starch	63:68	arg1	based					70:74	thermoplastic starch based	49:74	thermoplastic starch based	49:74	Water proof and strength retention properties of thermoplastic starch based biocomposites modified with glutaraldehyde.
25965466	1	49	theme	proof	126:130	arg1	properties					155:164	Water proof and strength retention properties	120:164	Water proof and strength retention properties of thermoplastic starch (TPS) resins	120:201	Water proof and strength retention properties of thermoplastic starch (TPS) resins were successfully improved by reacting glutaraldehyde (GA) with starch molecules during their gelatinization processes.
25965466	4	50	theme	starch	905:910	arg1	crystals					912:919	VH-type starch crystals	897:919	VH-type starch crystals	897:919	New melting endotherms and diffraction peaks of VH-type starch crystals were found on DSC thermograms and WAXD patterns of aged TPS or TPS100BC0.02 specimens, respectively, while negligible retrogradation effect was found for most aged TPS100BC0.02GAx and/or (TPS100BC0.02GAx)75PLA25 specimens.
25965466	3	51	theme	σf	664:665	arg1	values					677:682	The σf retention values	660:682	The σf retention values of (TPS100BC0.02GA0.5)75PLA25 specimen aged for 56 days	660:738	The σf retention values of (TPS100BC0.02GA0.5)75PLA25 specimen aged for 56 days are more than 50 times higher than those of corresponding aged TPS and TPS100BC0.02 specimens, respectively.
25965466	3	51	theme	σf	664:665	arg1	times					757:761	times	757:761	times	757:761	The σf retention values of (TPS100BC0.02GA0.5)75PLA25 specimen aged for 56 days are more than 50 times higher than those of corresponding aged TPS and TPS100BC0.02 specimens, respectively.
25965466	3	52	theme	retention	667:675	arg1	values					677:682	The σf retention values	660:682	The σf retention values of (TPS100BC0.02GA0.5)75PLA25 specimen aged for 56 days	660:738	The σf retention values of (TPS100BC0.02GA0.5)75PLA25 specimen aged for 56 days are more than 50 times higher than those of corresponding aged TPS and TPS100BC0.02 specimens, respectively.
25965466	3	52	theme	retention	667:675	arg1	times					757:761	times	757:761	times	757:761	The σf retention values of (TPS100BC0.02GA0.5)75PLA25 specimen aged for 56 days are more than 50 times higher than those of corresponding aged TPS and TPS100BC0.02 specimens, respectively.
25965466	1	53	theme	strength	136:143	arg1	retention					145:153	strength retention	136:153	strength retention	136:153	Water proof and strength retention properties of thermoplastic starch (TPS) resins were successfully improved by reacting glutaraldehyde (GA) with starch molecules during their gelatinization processes.
25965466	4	54	located	found	926:930	arg2	endotherms					861:870	New melting endotherms	849:870	New melting endotherms	849:870	New melting endotherms and diffraction peaks of VH-type starch crystals were found on DSC thermograms and WAXD patterns of aged TPS or TPS100BC0.02 specimens, respectively, while negligible retrogradation effect was found for most aged TPS100BC0.02GAx and/or (TPS100BC0.02GAx)75PLA25 specimens.
25965466	4	54	located	found	926:930	arg1	thermograms					939:949	DSC thermograms	935:949	DSC thermograms	935:949	New melting endotherms and diffraction peaks of VH-type starch crystals were found on DSC thermograms and WAXD patterns of aged TPS or TPS100BC0.02 specimens, respectively, while negligible retrogradation effect was found for most aged TPS100BC0.02GAx and/or (TPS100BC0.02GAx)75PLA25 specimens.
25965466	4	54	located	found	926:930	arg1	patterns					960:967	WAXD patterns	955:967	WAXD patterns	955:967	New melting endotherms and diffraction peaks of VH-type starch crystals were found on DSC thermograms and WAXD patterns of aged TPS or TPS100BC0.02 specimens, respectively, while negligible retrogradation effect was found for most aged TPS100BC0.02GAx and/or (TPS100BC0.02GAx)75PLA25 specimens.
25965466	4	54	located	found	926:930	arg2	peaks					888:892	diffraction peaks	876:892	diffraction peaks	876:892	New melting endotherms and diffraction peaks of VH-type starch crystals were found on DSC thermograms and WAXD patterns of aged TPS or TPS100BC0.02 specimens, respectively, while negligible retrogradation effect was found for most aged TPS100BC0.02GAx and/or (TPS100BC0.02GAx)75PLA25 specimens.
25965466	1	55	theme	retention	145:153	arg1	properties					155:164	Water proof and strength retention properties	120:164	Water proof and strength retention properties of thermoplastic starch (TPS) resins	120:201	Water proof and strength retention properties of thermoplastic starch (TPS) resins were successfully improved by reacting glutaraldehyde (GA) with starch molecules during their gelatinization processes.
25965466	3	56	theme	TPS100BC0.02	811:822	arg1	specimens					824:832	TPS100BC0.02 specimens	811:832	TPS100BC0.02 specimens	811:832	The σf retention values of (TPS100BC0.02GA0.5)75PLA25 specimen aged for 56 days are more than 50 times higher than those of corresponding aged TPS and TPS100BC0.02 specimens, respectively.
25965466	0	57	theme	based	70:74	arg1	properties					35:44	strength retention properties	16:44	strength retention properties	16:44	Water proof and strength retention properties of thermoplastic starch based biocomposites modified with glutaraldehyde.
25965466	0	57	theme	based	70:74	arg1	proof					6:10	Water proof	0:10	Water proof	0:10	Water proof and strength retention properties of thermoplastic starch based biocomposites modified with glutaraldehyde.
25965466	2	58	theme	TPS100BC0.02GAx	372:386	arg1	values					345:350	Tensile strength (σf) values	323:350	Tensile strength (σf) values of initial and aged TPS100BC0.02GAx and (TPS100BC0.02GAx)75PLA25 specimens	323:425	Tensile strength (σf) values of initial and aged TPS100BC0.02GAx and (TPS100BC0.02GAx)75PLA25 specimens improved significantly to a maximal value as GA contents approached an optimal value, while their moisture content and elongation at break values reduced to a minimal value, respectively, as GA contents approached the optimal value.
25965466	2	59	theme	TPS100BC0.02GAx	393:407	arg1	specimens					417:425	(TPS100BC0.02GAx)75PLA25 specimens	392:425	(TPS100BC0.02GAx)75PLA25 specimens	392:425	Tensile strength (σf) values of initial and aged TPS100BC0.02GAx and (TPS100BC0.02GAx)75PLA25 specimens improved significantly to a maximal value as GA contents approached an optimal value, while their moisture content and elongation at break values reduced to a minimal value, respectively, as GA contents approached the optimal value.
25965466	0	60	theme	thermoplastic	49:61	arg1	based					70:74	thermoplastic starch based	49:74	thermoplastic starch based	49:74	Water proof and strength retention properties of thermoplastic starch based biocomposites modified with glutaraldehyde.
25965466	2	61	theme	aged	367:370	arg1	TPS100BC0.02GAx					372:386	initial and aged TPS100BC0.02GAx	355:386	initial and aged TPS100BC0.02GAx	355:386	Tensile strength (σf) values of initial and aged TPS100BC0.02GAx and (TPS100BC0.02GAx)75PLA25 specimens improved significantly to a maximal value as GA contents approached an optimal value, while their moisture content and elongation at break values reduced to a minimal value, respectively, as GA contents approached the optimal value.
25965466	4	62	theme	negligible	1028:1037	arg1	effect					1054:1059	negligible retrogradation effect	1028:1059	negligible retrogradation effect	1028:1059	New melting endotherms and diffraction peaks of VH-type starch crystals were found on DSC thermograms and WAXD patterns of aged TPS or TPS100BC0.02 specimens, respectively, while negligible retrogradation effect was found for most aged TPS100BC0.02GAx and/or (TPS100BC0.02GAx)75PLA25 specimens.
27432416	0	0	theme	composites	108:117	arg1	studies					37:43	in vitro studies	28:43	in vitro studies	28:43	Rheological evaluations and in vitro studies of injectable bioactive glass-polycaprolactone-sodium alginate composites.
27432416	0	0	theme	composites	108:117	arg1	evaluations					12:22	Rheological evaluations	0:22	Rheological evaluations	0:22	Rheological evaluations and in vitro studies of injectable bioactive glass-polycaprolactone-sodium alginate composites.
27432416	1	1	theme	glass	191:195	arg1	MG5					203:205	MG5	203:205	MG5	203:205	Composite pastes composed of various amounts of melt-derived bioactive glass 52S4 (MG5) and polycaprolactone (PCL) microspheres in sodium alginate solution were prepared.
27432416	1	1	theme	glass	191:195	arg1	52S4					197:200	bioactive glass 52S4	181:200	bioactive glass 52S4 (MG5)	181:206	Composite pastes composed of various amounts of melt-derived bioactive glass 52S4 (MG5) and polycaprolactone (PCL) microspheres in sodium alginate solution were prepared.
27432416	10	2	theme	10-40 wt	1290:1297	arg1	PCL					1300:1302	10-40 wt% PCL	1290:1302	10-40 wt% PCL	1290:1302	In vitro calcium phosphate precipitation was well observed on the paste made of MG5 and somewhat on the pastes with 10-40 wt% PCL, however the precipitated layer was amorphous in nature.
27432416	0	3	theme	alginate	99:106	arg1	composites					108:117	injectable bioactive glass-polycaprolactone-sodium alginate composites	48:117	injectable bioactive glass-polycaprolactone-sodium alginate composites	48:117	Rheological evaluations and in vitro studies of injectable bioactive glass-polycaprolactone-sodium alginate composites.
27432416	4	4	theme	scanning	549:556	arg1	microscopy					567:576	scanning electron microscopy	549:576	scanning electron microscopy	549:576	In vitro calcium phosphate precipitation was also studied in simulated body fluid (SBF) and tracked using scanning electron microscopy, X-ray diffraction and FTIR analyses.
27432416	4	5	theme	In	443:444	arg1	precipitation					470:482	In vitro calcium phosphate precipitation	443:482	In vitro calcium phosphate precipitation	443:482	In vitro calcium phosphate precipitation was also studied in simulated body fluid (SBF) and tracked using scanning electron microscopy, X-ray diffraction and FTIR analyses.
27432416	1	6	theme	52S4	197:200	arg1	microspheres					235:246	melt-derived bioactive glass 52S4 (MG5) and polycaprolactone (PCL) microspheres	168:246	melt-derived bioactive glass 52S4 (MG5) and polycaprolactone (PCL) microspheres in sodium alginate solution	168:274	Composite pastes composed of various amounts of melt-derived bioactive glass 52S4 (MG5) and polycaprolactone (PCL) microspheres in sodium alginate solution were prepared.
27432416	4	7	dep	In	443:444	arg1	vitro					446:450	vitro	446:450	vitro	446:450	In vitro calcium phosphate precipitation was also studied in simulated body fluid (SBF) and tracked using scanning electron microscopy, X-ray diffraction and FTIR analyses.
27432416	8	8	theme	reasonable	1068:1077	arg1	injectability					1079:1091	reasonable injectability	1068:1091	reasonable injectability	1068:1091	The pastes which were just made of MG5 or PCL had poor injectability, whereas the composites containing both of these constituents exhibited reasonable injectability.
27432416	7	9	theme	viscous	913:919	arg1	term					921:924	viscous term	913:924	viscous term	913:924	Moreover, the composites were viscoelastic materials in which the elastic modulus was higher than viscous term.
27432416	3	10	theme	injection	399:407	arg1	force					409:413	injection force	399:413	injection force	399:413	Injectability was measured as injection force versus piston displacement.
27432416	3	10	theme	injection	399:407	arg1	Injectability					369:381	Injectability	369:381	Injectability	369:381	Injectability was measured as injection force versus piston displacement.
27432416	4	11	theme	calcium	452:458	arg1	precipitation					470:482	In vitro calcium phosphate precipitation	443:482	In vitro calcium phosphate precipitation	443:482	In vitro calcium phosphate precipitation was also studied in simulated body fluid (SBF) and tracked using scanning electron microscopy, X-ray diffraction and FTIR analyses.
27432416	6	12	theme	thixotropy	720:729	arg1	magnitude					707:715	The magnitude	703:715	The magnitude of thixotropy	703:729	The magnitude of thixotropy decreased by adding 10-30 wt% PCL, while further amounts of PCL increased it again.
27432416	11	13	theme	more	1471:1474	arg1	evaluations					1487:1497	more biological evaluations	1471:1497	more biological evaluations	1471:1497	Overall, the produced composites may be appropriate as injectable biomaterials for non-invasive surgeries but more biological evaluations are essential.
27432416	11	14	theme	injectable	1416:1425	arg1	biomaterials					1427:1438	injectable biomaterials	1416:1438	injectable biomaterials for non-invasive surgeries	1416:1465	Overall, the produced composites may be appropriate as injectable biomaterials for non-invasive surgeries but more biological evaluations are essential.
27432416	9	15	theme	adequate	1114:1121	arg1	stability					1134:1142	adequate structural stability	1114:1142	adequate structural stability	1114:1142	All pastes revealed adequate structural stability in contact with SBF solution.
27432416	6	16	theme	%	759:759	arg1	PCL					761:763	10-30 wt% PCL	751:763	10-30 wt% PCL	751:763	The magnitude of thixotropy decreased by adding 10-30 wt% PCL, while further amounts of PCL increased it again.
27432416	9	17	with	contact	1147:1153	arg1	solution					1164:1171	SBF solution	1160:1171	SBF solution	1160:1171	All pastes revealed adequate structural stability in contact with SBF solution.
27432416	1	18	from	amounts	157:163	arg1	solution					267:274	sodium alginate solution	251:274	sodium alginate solution	251:274	Composite pastes composed of various amounts of melt-derived bioactive glass 52S4 (MG5) and polycaprolactone (PCL) microspheres in sodium alginate solution were prepared.
27432416	0	19	theme	Rheological	0:10	arg1	evaluations					12:22	Rheological evaluations	0:22	Rheological evaluations	0:22	Rheological evaluations and in vitro studies of injectable bioactive glass-polycaprolactone-sodium alginate composites.
27432416	6	20	theme	10-30 wt	751:758	arg1	PCL					761:763	10-30 wt% PCL	751:763	10-30 wt% PCL	751:763	The magnitude of thixotropy decreased by adding 10-30 wt% PCL, while further amounts of PCL increased it again.
27432416	10	21	theme	phosphate	1191:1199	arg1	precipitation					1201:1213	In vitro calcium phosphate precipitation	1174:1213	In vitro calcium phosphate precipitation	1174:1213	In vitro calcium phosphate precipitation was well observed on the paste made of MG5 and somewhat on the pastes with 10-40 wt% PCL, however the precipitated layer was amorphous in nature.
27432416	3	22	theme	piston	422:427	arg1	displacement					429:440	piston displacement	422:440	piston displacement	422:440	Injectability was measured as injection force versus piston displacement.
27432416	3	22	theme	piston	422:427	arg1	Injectability					369:381	Injectability	369:381	Injectability	369:381	Injectability was measured as injection force versus piston displacement.
27432416	10	23	dep	In	1174:1175	arg1	vitro					1177:1181	vitro	1177:1181	vitro	1177:1181	In vitro calcium phosphate precipitation was well observed on the paste made of MG5 and somewhat on the pastes with 10-40 wt% PCL, however the precipitated layer was amorphous in nature.
27432416	5	24	theme	shear	678:682	arg1	behavior					693:700	shear thinning behavior	678:700	shear thinning behavior	678:700	All composite pastes were thixotropic in nature and exhibited shear thinning behavior.
27432416	9	25	theme	structural	1123:1132	arg1	stability					1134:1142	adequate structural stability	1114:1142	adequate structural stability	1114:1142	All pastes revealed adequate structural stability in contact with SBF solution.
27432416	1	26	from	solution	267:274	arg1	amounts					157:163	various amounts	149:163	various amounts of melt-derived bioactive glass 52S4 (MG5) and polycaprolactone (PCL) microspheres in sodium alginate solution	149:274	Composite pastes composed of various amounts of melt-derived bioactive glass 52S4 (MG5) and polycaprolactone (PCL) microspheres in sodium alginate solution were prepared.
27432416	1	26	from	solution	267:274	arg1	microspheres					235:246	melt-derived bioactive glass 52S4 (MG5) and polycaprolactone (PCL) microspheres	168:246	melt-derived bioactive glass 52S4 (MG5) and polycaprolactone (PCL) microspheres in sodium alginate solution	168:274	Composite pastes composed of various amounts of melt-derived bioactive glass 52S4 (MG5) and polycaprolactone (PCL) microspheres in sodium alginate solution were prepared.
27432416	8	27	contain	had	973:975	arg1	pastes					931:936	The pastes	927:936	The pastes which were just made of MG5 or PCL	927:971	The pastes which were just made of MG5 or PCL had poor injectability, whereas the composites containing both of these constituents exhibited reasonable injectability.
27432416	8	27	contain	had	973:975	arg2	injectability					982:994	poor injectability	977:994	poor injectability	977:994	The pastes which were just made of MG5 or PCL had poor injectability, whereas the composites containing both of these constituents exhibited reasonable injectability.
27432416	5	28	theme	thinning	684:691	arg1	behavior					693:700	shear thinning behavior	678:700	shear thinning behavior	678:700	All composite pastes were thixotropic in nature and exhibited shear thinning behavior.
27432416	5	29	theme	composite	620:628	arg1	pastes					630:635	All composite pastes	616:635	All composite pastes	616:635	All composite pastes were thixotropic in nature and exhibited shear thinning behavior.
27432416	1	30	theme	Composite	120:128	arg1	pastes					130:135	Composite pastes	120:135	Composite pastes composed of various amounts of melt-derived bioactive glass 52S4 (MG5) and polycaprolactone (PCL) microspheres in sodium alginate solution	120:274	Composite pastes composed of various amounts of melt-derived bioactive glass 52S4 (MG5) and polycaprolactone (PCL) microspheres in sodium alginate solution were prepared.
27432416	0	31	theme	in	28:29	arg1	studies					37:43	in vitro studies	28:43	in vitro studies	28:43	Rheological evaluations and in vitro studies of injectable bioactive glass-polycaprolactone-sodium alginate composites.
27432416	7	32	theme	viscoelastic	845:856	arg1	composites					829:838	the composites	825:838	the composites	825:838	Moreover, the composites were viscoelastic materials in which the elastic modulus was higher than viscous term.
27432416	7	32	theme	viscoelastic	845:856	arg1	materials					858:866	viscoelastic materials	845:866	viscoelastic materials in which the elastic modulus was higher than viscous term	845:924	Moreover, the composites were viscoelastic materials in which the elastic modulus was higher than viscous term.
27432416	10	33	located	observed	1224:1231	arg2	precipitation					1201:1213	In vitro calcium phosphate precipitation	1174:1213	In vitro calcium phosphate precipitation	1174:1213	In vitro calcium phosphate precipitation was well observed on the paste made of MG5 and somewhat on the pastes with 10-40 wt% PCL, however the precipitated layer was amorphous in nature.
27432416	10	33	located	observed	1224:1231	arg1	paste					1240:1244	the paste	1236:1244	the paste made of MG5	1236:1256	In vitro calcium phosphate precipitation was well observed on the paste made of MG5 and somewhat on the pastes with 10-40 wt% PCL, however the precipitated layer was amorphous in nature.
27432416	1	34	theme	polycaprolactone	212:227	arg1	microspheres					235:246	melt-derived bioactive glass 52S4 (MG5) and polycaprolactone (PCL) microspheres	168:246	melt-derived bioactive glass 52S4 (MG5) and polycaprolactone (PCL) microspheres in sodium alginate solution	168:274	Composite pastes composed of various amounts of melt-derived bioactive glass 52S4 (MG5) and polycaprolactone (PCL) microspheres in sodium alginate solution were prepared.
27432416	2	35	theme	oscillatory	335:345	arg1	modes					347:351	both rotatory and oscillatory modes	317:351	both rotatory and oscillatory modes	317:351	Rheological properties in both rotatory and oscillatory modes were evaluated.
27432416	10	36	dep	PCL	1300:1302	arg1	layer					1330:1334	the precipitated layer	1313:1334	the precipitated layer	1313:1334	In vitro calcium phosphate precipitation was well observed on the paste made of MG5 and somewhat on the pastes with 10-40 wt% PCL, however the precipitated layer was amorphous in nature.
27432416	10	36	dep	PCL	1300:1302	arg1	amorphous					1340:1348	amorphous	1340:1348	amorphous	1340:1348	In vitro calcium phosphate precipitation was well observed on the paste made of MG5 and somewhat on the pastes with 10-40 wt% PCL, however the precipitated layer was amorphous in nature.
27432416	2	37	theme	rotatory	322:329	arg1	modes					347:351	both rotatory and oscillatory modes	317:351	both rotatory and oscillatory modes	317:351	Rheological properties in both rotatory and oscillatory modes were evaluated.
27432416	5	38	from	thixotropic	642:652	arg1	nature					657:662	nature	657:662	nature	657:662	All composite pastes were thixotropic in nature and exhibited shear thinning behavior.
27432416	11	39	theme	produced	1374:1381	arg1	appropriate					1401:1411	appropriate	1401:1411	appropriate	1401:1411	Overall, the produced composites may be appropriate as injectable biomaterials for non-invasive surgeries but more biological evaluations are essential.
27432416	11	39	theme	produced	1374:1381	arg1	composites					1383:1392	the produced composites	1370:1392	the produced composites	1370:1392	Overall, the produced composites may be appropriate as injectable biomaterials for non-invasive surgeries but more biological evaluations are essential.
27432416	4	40	theme	FTIR	601:604	arg1	analyses					606:613	FTIR analyses	601:613	FTIR analyses	601:613	In vitro calcium phosphate precipitation was also studied in simulated body fluid (SBF) and tracked using scanning electron microscopy, X-ray diffraction and FTIR analyses.
27432416	1	41	link	melt-derived	168:179	arg1	microspheres					235:246	melt-derived bioactive glass 52S4 (MG5) and polycaprolactone (PCL) microspheres	168:246	melt-derived bioactive glass 52S4 (MG5) and polycaprolactone (PCL) microspheres in sodium alginate solution	168:274	Composite pastes composed of various amounts of melt-derived bioactive glass 52S4 (MG5) and polycaprolactone (PCL) microspheres in sodium alginate solution were prepared.
27432416	9	42	theme	SBF	1160:1162	arg1	solution					1164:1171	SBF solution	1160:1171	SBF solution	1160:1171	All pastes revealed adequate structural stability in contact with SBF solution.
27432416	11	43	theme	biological	1476:1485	arg1	evaluations					1487:1497	more biological evaluations	1471:1497	more biological evaluations	1471:1497	Overall, the produced composites may be appropriate as injectable biomaterials for non-invasive surgeries but more biological evaluations are essential.
27432416	10	44	from	nature	1353:1358	arg1	layer					1330:1334	the precipitated layer	1313:1334	the precipitated layer	1313:1334	In vitro calcium phosphate precipitation was well observed on the paste made of MG5 and somewhat on the pastes with 10-40 wt% PCL, however the precipitated layer was amorphous in nature.
27432416	10	44	from	nature	1353:1358	arg1	amorphous					1340:1348	amorphous	1340:1348	amorphous	1340:1348	In vitro calcium phosphate precipitation was well observed on the paste made of MG5 and somewhat on the pastes with 10-40 wt% PCL, however the precipitated layer was amorphous in nature.
27432416	4	45	theme	body	514:517	arg1	SBF					526:528	SBF	526:528	SBF	526:528	In vitro calcium phosphate precipitation was also studied in simulated body fluid (SBF) and tracked using scanning electron microscopy, X-ray diffraction and FTIR analyses.
27432416	4	45	theme	body	514:517	arg1	fluid					519:523	simulated body fluid	504:523	simulated body fluid (SBF)	504:529	In vitro calcium phosphate precipitation was also studied in simulated body fluid (SBF) and tracked using scanning electron microscopy, X-ray diffraction and FTIR analyses.
27432416	10	46	theme	In	1174:1175	arg1	precipitation					1201:1213	In vitro calcium phosphate precipitation	1174:1213	In vitro calcium phosphate precipitation	1174:1213	In vitro calcium phosphate precipitation was well observed on the paste made of MG5 and somewhat on the pastes with 10-40 wt% PCL, however the precipitated layer was amorphous in nature.
27432416	0	47	theme	injectable	48:57	arg1	composites					108:117	injectable bioactive glass-polycaprolactone-sodium alginate composites	48:117	injectable bioactive glass-polycaprolactone-sodium alginate composites	48:117	Rheological evaluations and in vitro studies of injectable bioactive glass-polycaprolactone-sodium alginate composites.
27432416	4	48	theme	electron	558:565	arg1	microscopy					567:576	scanning electron microscopy	549:576	scanning electron microscopy	549:576	In vitro calcium phosphate precipitation was also studied in simulated body fluid (SBF) and tracked using scanning electron microscopy, X-ray diffraction and FTIR analyses.
27432416	10	49	theme	precipitated	1317:1328	arg1	layer					1330:1334	the precipitated layer	1313:1334	the precipitated layer	1313:1334	In vitro calcium phosphate precipitation was well observed on the paste made of MG5 and somewhat on the pastes with 10-40 wt% PCL, however the precipitated layer was amorphous in nature.
27432416	10	49	theme	precipitated	1317:1328	arg1	amorphous					1340:1348	amorphous	1340:1348	amorphous	1340:1348	In vitro calcium phosphate precipitation was well observed on the paste made of MG5 and somewhat on the pastes with 10-40 wt% PCL, however the precipitated layer was amorphous in nature.
27432416	4	50	theme	simulated	504:512	arg1	SBF					526:528	SBF	526:528	SBF	526:528	In vitro calcium phosphate precipitation was also studied in simulated body fluid (SBF) and tracked using scanning electron microscopy, X-ray diffraction and FTIR analyses.
27432416	4	50	theme	simulated	504:512	arg1	fluid					519:523	simulated body fluid	504:523	simulated body fluid (SBF)	504:529	In vitro calcium phosphate precipitation was also studied in simulated body fluid (SBF) and tracked using scanning electron microscopy, X-ray diffraction and FTIR analyses.
27432416	4	51	theme	X-ray	579:583	arg1	diffraction					585:595	X-ray diffraction	579:595	X-ray diffraction	579:595	In vitro calcium phosphate precipitation was also studied in simulated body fluid (SBF) and tracked using scanning electron microscopy, X-ray diffraction and FTIR analyses.
27432416	10	52	with	pastes	1278:1283	arg1	PCL					1300:1302	10-40 wt% PCL	1290:1302	10-40 wt% PCL	1290:1302	In vitro calcium phosphate precipitation was well observed on the paste made of MG5 and somewhat on the pastes with 10-40 wt% PCL, however the precipitated layer was amorphous in nature.
27432416	6	53	theme	PCL	791:793	arg1	PCL					791:793	PCL	791:793	PCL	791:793	The magnitude of thixotropy decreased by adding 10-30 wt% PCL, while further amounts of PCL increased it again.
27432416	6	53	theme	PCL	791:793	arg1	amounts					780:786	further amounts	772:786	further amounts of PCL	772:793	The magnitude of thixotropy decreased by adding 10-30 wt% PCL, while further amounts of PCL increased it again.
27432416	1	54	theme	various	149:155	arg1	amounts					157:163	various amounts	149:163	various amounts of melt-derived bioactive glass 52S4 (MG5) and polycaprolactone (PCL) microspheres in sodium alginate solution	149:274	Composite pastes composed of various amounts of melt-derived bioactive glass 52S4 (MG5) and polycaprolactone (PCL) microspheres in sodium alginate solution were prepared.
27432416	1	54	theme	various	149:155	arg1	microspheres					235:246	melt-derived bioactive glass 52S4 (MG5) and polycaprolactone (PCL) microspheres	168:246	melt-derived bioactive glass 52S4 (MG5) and polycaprolactone (PCL) microspheres in sodium alginate solution	168:274	Composite pastes composed of various amounts of melt-derived bioactive glass 52S4 (MG5) and polycaprolactone (PCL) microspheres in sodium alginate solution were prepared.
27432416	2	55	from	properties	303:312	arg1	modes					347:351	both rotatory and oscillatory modes	317:351	both rotatory and oscillatory modes	317:351	Rheological properties in both rotatory and oscillatory modes were evaluated.
27432416	10	56	theme	calcium	1183:1189	arg1	precipitation					1201:1213	In vitro calcium phosphate precipitation	1174:1213	In vitro calcium phosphate precipitation	1174:1213	In vitro calcium phosphate precipitation was well observed on the paste made of MG5 and somewhat on the pastes with 10-40 wt% PCL, however the precipitated layer was amorphous in nature.
27432416	1	57	from	microspheres	235:246	arg1	solution					267:274	sodium alginate solution	251:274	sodium alginate solution	251:274	Composite pastes composed of various amounts of melt-derived bioactive glass 52S4 (MG5) and polycaprolactone (PCL) microspheres in sodium alginate solution were prepared.
27432416	4	58	theme	phosphate	460:468	arg1	precipitation					470:482	In vitro calcium phosphate precipitation	443:482	In vitro calcium phosphate precipitation	443:482	In vitro calcium phosphate precipitation was also studied in simulated body fluid (SBF) and tracked using scanning electron microscopy, X-ray diffraction and FTIR analyses.
27432416	0	59	dep	in	28:29	arg1	vitro					31:35	vitro	31:35	vitro	31:35	Rheological evaluations and in vitro studies of injectable bioactive glass-polycaprolactone-sodium alginate composites.
27432416	1	60	theme	microspheres	235:246	arg1	amounts					157:163	various amounts	149:163	various amounts of melt-derived bioactive glass 52S4 (MG5) and polycaprolactone (PCL) microspheres in sodium alginate solution	149:274	Composite pastes composed of various amounts of melt-derived bioactive glass 52S4 (MG5) and polycaprolactone (PCL) microspheres in sodium alginate solution were prepared.
27432416	1	60	theme	microspheres	235:246	arg1	microspheres					235:246	melt-derived bioactive glass 52S4 (MG5) and polycaprolactone (PCL) microspheres	168:246	melt-derived bioactive glass 52S4 (MG5) and polycaprolactone (PCL) microspheres in sodium alginate solution	168:274	Composite pastes composed of various amounts of melt-derived bioactive glass 52S4 (MG5) and polycaprolactone (PCL) microspheres in sodium alginate solution were prepared.
27432416	2	61	theme	Rheological	291:301	arg1	properties					303:312	Rheological properties	291:312	Rheological properties in both rotatory and oscillatory modes	291:351	Rheological properties in both rotatory and oscillatory modes were evaluated.
27432416	11	62	theme	non-invasive	1444:1455	arg1	surgeries					1457:1465	non-invasive surgeries	1444:1465	non-invasive surgeries	1444:1465	Overall, the produced composites may be appropriate as injectable biomaterials for non-invasive surgeries but more biological evaluations are essential.
27432416	5	63	from	nature	657:662	arg1	thixotropic					642:652	thixotropic	642:652	thixotropic	642:652	All composite pastes were thixotropic in nature and exhibited shear thinning behavior.
27432416	7	64	theme	elastic	881:887	arg1	modulus					889:895	the elastic modulus	877:895	the elastic modulus	877:895	Moreover, the composites were viscoelastic materials in which the elastic modulus was higher than viscous term.
27432416	7	64	theme	elastic	881:887	arg1	higher					901:906	higher	901:906	higher	901:906	Moreover, the composites were viscoelastic materials in which the elastic modulus was higher than viscous term.
27432416	0	65	theme	glass-polycaprolactone-sodium	69:97	arg1	composites					108:117	injectable bioactive glass-polycaprolactone-sodium alginate composites	48:117	injectable bioactive glass-polycaprolactone-sodium alginate composites	48:117	Rheological evaluations and in vitro studies of injectable bioactive glass-polycaprolactone-sodium alginate composites.
27432416	10	66	from	amorphous	1340:1348	arg1	nature					1353:1358	nature	1353:1358	nature	1353:1358	In vitro calcium phosphate precipitation was well observed on the paste made of MG5 and somewhat on the pastes with 10-40 wt% PCL, however the precipitated layer was amorphous in nature.
27432416	6	67	theme	further	772:778	arg1	PCL					791:793	PCL	791:793	PCL	791:793	The magnitude of thixotropy decreased by adding 10-30 wt% PCL, while further amounts of PCL increased it again.
27432416	6	67	theme	further	772:778	arg1	amounts					780:786	further amounts	772:786	further amounts of PCL	772:793	The magnitude of thixotropy decreased by adding 10-30 wt% PCL, while further amounts of PCL increased it again.
27432416	1	68	theme	melt-derived	168:179	arg1	microspheres					235:246	melt-derived bioactive glass 52S4 (MG5) and polycaprolactone (PCL) microspheres	168:246	melt-derived bioactive glass 52S4 (MG5) and polycaprolactone (PCL) microspheres in sodium alginate solution	168:274	Composite pastes composed of various amounts of melt-derived bioactive glass 52S4 (MG5) and polycaprolactone (PCL) microspheres in sodium alginate solution were prepared.
27432416	1	69	theme	sodium	251:256	arg1	solution					267:274	sodium alginate solution	251:274	sodium alginate solution	251:274	Composite pastes composed of various amounts of melt-derived bioactive glass 52S4 (MG5) and polycaprolactone (PCL) microspheres in sodium alginate solution were prepared.
27432416	0	70	theme	bioactive	59:67	arg1	composites					108:117	injectable bioactive glass-polycaprolactone-sodium alginate composites	48:117	injectable bioactive glass-polycaprolactone-sodium alginate composites	48:117	Rheological evaluations and in vitro studies of injectable bioactive glass-polycaprolactone-sodium alginate composites.
27432416	8	71	contain	containing	1020:1029	arg2	both					1031:1034	both	1031:1034	both	1031:1034	The pastes which were just made of MG5 or PCL had poor injectability, whereas the composites containing both of these constituents exhibited reasonable injectability.
27432416	8	71	contain	containing	1020:1029	arg2	constituents					1045:1056	these constituents	1039:1056	these constituents	1039:1056	The pastes which were just made of MG5 or PCL had poor injectability, whereas the composites containing both of these constituents exhibited reasonable injectability.
27432416	8	71	contain	containing	1020:1029	arg1	composites					1009:1018	the composites	1005:1018	the composites containing both of these constituents	1005:1056	The pastes which were just made of MG5 or PCL had poor injectability, whereas the composites containing both of these constituents exhibited reasonable injectability.
27432416	8	72	theme	poor	977:980	arg1	injectability					982:994	poor injectability	977:994	poor injectability	977:994	The pastes which were just made of MG5 or PCL had poor injectability, whereas the composites containing both of these constituents exhibited reasonable injectability.
27432416	1	73	theme	bioactive	181:189	arg1	MG5					203:205	MG5	203:205	MG5	203:205	Composite pastes composed of various amounts of melt-derived bioactive glass 52S4 (MG5) and polycaprolactone (PCL) microspheres in sodium alginate solution were prepared.
27432416	1	73	theme	bioactive	181:189	arg1	52S4					197:200	bioactive glass 52S4	181:200	bioactive glass 52S4 (MG5)	181:206	Composite pastes composed of various amounts of melt-derived bioactive glass 52S4 (MG5) and polycaprolactone (PCL) microspheres in sodium alginate solution were prepared.
27432416	10	74	theme	%	1298:1298	arg1	PCL					1300:1302	10-40 wt% PCL	1290:1302	10-40 wt% PCL	1290:1302	In vitro calcium phosphate precipitation was well observed on the paste made of MG5 and somewhat on the pastes with 10-40 wt% PCL, however the precipitated layer was amorphous in nature.
27432416	1	75	theme	alginate	258:265	arg1	solution					267:274	sodium alginate solution	251:274	sodium alginate solution	251:274	Composite pastes composed of various amounts of melt-derived bioactive glass 52S4 (MG5) and polycaprolactone (PCL) microspheres in sodium alginate solution were prepared.
26376751	10	0	theme	massive	1796:1802	arg1	infusion					1816:1823	massive immune cell infusion	1796:1823	massive immune cell infusion	1796:1823	The histochemical analysis revealed that after 8 weeks, the biodegradation of PLA leads to massive immune cell infusion and inflammation at the site, whereas the PPC composites are well-tolerated in vivo.
26376751	11	1	theme	benign	1993:1998	arg1	biomaterial					2014:2024	a benign biodegradable biomaterial	1991:2024	a benign biodegradable biomaterial for fabrication of biomedical implants	1991:2063	All these results underline the favorable properties of PPC-starch composites as a benign biodegradable biomaterial for fabrication of biomedical implants.
26376751	11	1	theme	benign	1993:1998	arg1	properties					1952:1961	the favorable properties	1938:1961	the favorable properties of PPC-starch composites	1938:1986	All these results underline the favorable properties of PPC-starch composites as a benign biodegradable biomaterial for fabrication of biomedical implants.
26376751	11	2	theme	biomedical	2045:2054	arg1	implants					2056:2063	biomedical implants	2045:2063	biomedical implants	2045:2063	All these results underline the favorable properties of PPC-starch composites as a benign biodegradable biomaterial for fabrication of biomedical implants.
26376751	3	3	theme	PPC-starch	496:505	arg1	composites					507:516	PPC-starch composites	496:516	PPC-starch composites	496:516	The degradation products of PPC-starch composites are mainly carbon dioxide and water; hence, the associated risks to the acidic degradation of polyesters are minimized.
26376751	7	4	theme	cells	1355:1359	arg1	numbers					1333:1339	the numbers	1329:1339	the numbers of osteoblast cells	1329:1359	For instance, the numbers of osteoblast cells were increased 2.5 fold after 7 days post culture.
26376751	1	5	theme	degradation	153:163	arg1	products					165:172	the degradation products	149:172	the degradation products of polyesters	149:186	The acidic nature of the degradation products of polyesters often leads to unpredictable clinical complications, such as necrosis of host tissues and massive immune cell invasions.
26376751	9	6	theme	in	1621:1622	arg1	biodegradation					1629:1642	The long-term in vivo biodegradation	1607:1642	The long-term in vivo biodegradation of PPC composites	1607:1660	The long-term in vivo biodegradation of PPC composites are compared with poly(lactic acid) (PLA).
26376751	8	7	dep	formation	1518:1526	arg1	e.g.					1508:1511	e.g.	1508:1511	e.g.	1508:1511	In addition, PPC composites in subcutaneous mice model resulted in mild inflammatory responses (e.g., the formation of fibrotic tissue) that were diminished from two to 4 weeks postimplantation.
26376751	3	8	theme	carbon	529:534	arg1	dioxide					536:542	carbon dioxide	529:542	carbon dioxide	529:542	The degradation products of PPC-starch composites are mainly carbon dioxide and water; hence, the associated risks to the acidic degradation of polyesters are minimized.
26376751	3	8	theme	carbon	529:534	arg1	products					484:491	The degradation products	468:491	The degradation products of PPC-starch composites	468:516	The degradation products of PPC-starch composites are mainly carbon dioxide and water; hence, the associated risks to the acidic degradation of polyesters are minimized.
26376751	0	9	theme	lactic	114:119	arg1	Poly					109:112	Poly	109:112	Poly(lactic Acid)	109:125	Reinforced Poly(Propylene Carbonate) Composite with Enhanced and Tunable Characteristics, an Alternative for Poly(lactic Acid).
26376751	0	9	theme	lactic	114:119	arg1	Acid					121:124	lactic Acid	114:124	lactic Acid	114:124	Reinforced Poly(Propylene Carbonate) Composite with Enhanced and Tunable Characteristics, an Alternative for Poly(lactic Acid).
26376751	8	10	from	composites	1429:1438	arg1	model					1461:1465	subcutaneous mice model	1443:1465	subcutaneous mice model	1443:1465	In addition, PPC composites in subcutaneous mice model resulted in mild inflammatory responses (e.g., the formation of fibrotic tissue) that were diminished from two to 4 weeks postimplantation.
26376751	6	11	dep	in	1300:1301	arg1	vivo					1303:1306	vivo	1303:1306	vivo	1303:1306	We also demonstrated the cytocompatibility and biocompatibility of these composites by conducting in vitro and in vivo tests.
26376751	9	12	theme	PPC	1647:1649	arg1	composites					1651:1660	PPC composites	1647:1660	PPC composites	1647:1660	The long-term in vivo biodegradation of PPC composites are compared with poly(lactic acid) (PLA).
26376751	10	13	theme	PPC	1867:1869	arg1	well-tolerated					1886:1899	well-tolerated	1886:1899	well-tolerated	1886:1899	The histochemical analysis revealed that after 8 weeks, the biodegradation of PLA leads to massive immune cell infusion and inflammation at the site, whereas the PPC composites are well-tolerated in vivo.
26376751	10	13	theme	PPC	1867:1869	arg1	composites					1871:1880	the PPC composites	1863:1880	the PPC composites	1863:1880	The histochemical analysis revealed that after 8 weeks, the biodegradation of PLA leads to massive immune cell infusion and inflammation at the site, whereas the PPC composites are well-tolerated in vivo.
26376751	11	14	theme	PPC-starch	1966:1975	arg1	composites					1977:1986	PPC-starch composites	1966:1986	PPC-starch composites	1966:1986	All these results underline the favorable properties of PPC-starch composites as a benign biodegradable biomaterial for fabrication of biomedical implants.
26376751	4	15	theme	composites	687:696	arg1	strength					664:671	the compression strength	648:671	the compression strength of PPC-starch composites	648:696	Moreover, the compression strength of PPC-starch composites can be tuned over the range of 0.2±0.03 MPa to 33.9±1.51 MPa by changing the starch contents of composites to address different clinical needs.
26376751	8	16	dep	4	1581:1581	arg1	to					1578:1579	to	1578:1579	to	1578:1579	In addition, PPC composites in subcutaneous mice model resulted in mild inflammatory responses (e.g., the formation of fibrotic tissue) that were diminished from two to 4 weeks postimplantation.
26376751	3	17	theme	associated	566:575	arg1	risks					577:581	the associated risks	562:581	the associated risks to the acidic degradation of polyesters	562:621	The degradation products of PPC-starch composites are mainly carbon dioxide and water; hence, the associated risks to the acidic degradation of polyesters are minimized.
26376751	8	18	theme	PPC	1425:1427	arg1	composites					1429:1438	PPC composites	1425:1438	PPC composites in subcutaneous mice model	1425:1465	In addition, PPC composites in subcutaneous mice model resulted in mild inflammatory responses (e.g., the formation of fibrotic tissue) that were diminished from two to 4 weeks postimplantation.
26376751	4	19	theme	compression	652:662	arg1	strength					664:671	the compression strength	648:671	the compression strength of PPC-starch composites	648:696	Moreover, the compression strength of PPC-starch composites can be tuned over the range of 0.2±0.03 MPa to 33.9±1.51 MPa by changing the starch contents of composites to address different clinical needs.
26376751	0	20	theme	Tunable	65:71	arg1	Characteristics					73:87	Tunable Characteristics	65:87	Tunable Characteristics	65:87	Reinforced Poly(Propylene Carbonate) Composite with Enhanced and Tunable Characteristics, an Alternative for Poly(lactic Acid).
26376751	1	21	theme	acidic	132:137	arg1	nature					139:144	The acidic nature	128:144	The acidic nature of the degradation products of polyesters	128:186	The acidic nature of the degradation products of polyesters often leads to unpredictable clinical complications, such as necrosis of host tissues and massive immune cell invasions.
26376751	5	22	theme	processing	1034:1043	arg1	extrusion					1062:1070	extrusion	1062:1070	extrusion	1062:1070	More importantly, the addition of 50 wt % starch enhances the thermal processing capacity of the composites by elevating their decomposition temperature from 245 to 276 °C. Therefore, thermal processing methods, such as extrusion and hot melt compression methods can be used to generate different shapes and structures from PPC-starch composites.
26376751	5	22	theme	processing	1034:1043	arg1	methods					1045:1051	thermal processing methods	1026:1051	thermal processing methods	1026:1051	More importantly, the addition of 50 wt % starch enhances the thermal processing capacity of the composites by elevating their decomposition temperature from 245 to 276 °C. Therefore, thermal processing methods, such as extrusion and hot melt compression methods can be used to generate different shapes and structures from PPC-starch composites.
26376751	5	22	theme	processing	1034:1043	arg1	compression					1085:1095	hot melt compression	1076:1095	hot melt compression	1076:1095	More importantly, the addition of 50 wt % starch enhances the thermal processing capacity of the composites by elevating their decomposition temperature from 245 to 276 °C. Therefore, thermal processing methods, such as extrusion and hot melt compression methods can be used to generate different shapes and structures from PPC-starch composites.
26376751	5	23	theme	thermal	904:910	arg1	capacity					923:930	the thermal processing capacity	900:930	the thermal processing capacity of the composites	900:948	More importantly, the addition of 50 wt % starch enhances the thermal processing capacity of the composites by elevating their decomposition temperature from 245 to 276 °C. Therefore, thermal processing methods, such as extrusion and hot melt compression methods can be used to generate different shapes and structures from PPC-starch composites.
26376751	8	24	theme	mice	1456:1459	arg1	model					1461:1465	subcutaneous mice model	1443:1465	subcutaneous mice model	1443:1465	In addition, PPC composites in subcutaneous mice model resulted in mild inflammatory responses (e.g., the formation of fibrotic tissue) that were diminished from two to 4 weeks postimplantation.
26376751	5	25	theme	%	882:882	arg1	starch					884:889	50 wt % starch	876:889	50 wt % starch	876:889	More importantly, the addition of 50 wt % starch enhances the thermal processing capacity of the composites by elevating their decomposition temperature from 245 to 276 °C. Therefore, thermal processing methods, such as extrusion and hot melt compression methods can be used to generate different shapes and structures from PPC-starch composites.
26376751	8	26	theme	mild	1479:1482	arg1	responses					1497:1505	mild inflammatory responses	1479:1505	mild inflammatory responses (e.g., the formation of fibrotic tissue) that were diminished from two to 4 weeks postimplantation	1479:1604	In addition, PPC composites in subcutaneous mice model resulted in mild inflammatory responses (e.g., the formation of fibrotic tissue) that were diminished from two to 4 weeks postimplantation.
26376751	2	27	theme	starch	360:365	arg1	composite					367:375	starch composite	360:375	starch composite	360:375	In this study, poly(propylene carbonate) (PPC) and starch composite is introduced with superior characteristics as an alternative to polyester-based polymers.
26376751	2	27	theme	starch	360:365	arg1	poly					324:327	poly	324:327	poly(propylene carbonate) (PPC)	324:354	In this study, poly(propylene carbonate) (PPC) and starch composite is introduced with superior characteristics as an alternative to polyester-based polymers.
26376751	2	27	theme	starch	360:365	arg1	alternative					427:437	an alternative	424:437	an alternative to polyester-based polymers	424:465	In this study, poly(propylene carbonate) (PPC) and starch composite is introduced with superior characteristics as an alternative to polyester-based polymers.
26376751	1	28	theme	host	261:264	arg1	tissues					266:272	host tissues	261:272	host tissues	261:272	The acidic nature of the degradation products of polyesters often leads to unpredictable clinical complications, such as necrosis of host tissues and massive immune cell invasions.
26376751	5	29	dep	extrusion	1062:1070	arg1	methods					1097:1103	methods	1097:1103	methods	1097:1103	More importantly, the addition of 50 wt % starch enhances the thermal processing capacity of the composites by elevating their decomposition temperature from 245 to 276 °C. Therefore, thermal processing methods, such as extrusion and hot melt compression methods can be used to generate different shapes and structures from PPC-starch composites.
26376751	0	30	theme	Carbonate	26:34	arg1	Composite					37:45	Poly(Propylene Carbonate) Composite	11:45	Poly(Propylene Carbonate) Composite with Enhanced and Tunable Characteristics, an Alternative for Poly(lactic Acid)	11:125	Reinforced Poly(Propylene Carbonate) Composite with Enhanced and Tunable Characteristics, an Alternative for Poly(lactic Acid).
26376751	5	31	theme	melt	1080:1083	arg1	compression					1085:1095	hot melt compression	1076:1095	hot melt compression	1076:1095	More importantly, the addition of 50 wt % starch enhances the thermal processing capacity of the composites by elevating their decomposition temperature from 245 to 276 °C. Therefore, thermal processing methods, such as extrusion and hot melt compression methods can be used to generate different shapes and structures from PPC-starch composites.
26376751	4	32	dep	clinical	826:833	arg1	needs					835:839	needs	835:839	needs	835:839	Moreover, the compression strength of PPC-starch composites can be tuned over the range of 0.2±0.03 MPa to 33.9±1.51 MPa by changing the starch contents of composites to address different clinical needs.
26376751	3	33	theme	acidic	590:595	arg1	degradation					597:607	the acidic degradation	586:607	the acidic degradation of polyesters	586:621	The degradation products of PPC-starch composites are mainly carbon dioxide and water; hence, the associated risks to the acidic degradation of polyesters are minimized.
26376751	4	34	theme	starch	775:780	arg1	contents					782:789	the starch contents	771:789	the starch contents of composites to address different clinical needs	771:839	Moreover, the compression strength of PPC-starch composites can be tuned over the range of 0.2±0.03 MPa to 33.9±1.51 MPa by changing the starch contents of composites to address different clinical needs.
26376751	1	35	theme	immune	286:291	arg1	invasions					298:306	massive immune cell invasions	278:306	massive immune cell invasions	278:306	The acidic nature of the degradation products of polyesters often leads to unpredictable clinical complications, such as necrosis of host tissues and massive immune cell invasions.
26376751	10	36	theme	PLA	1783:1785	arg1	biodegradation					1765:1778	the biodegradation	1761:1778	the biodegradation of PLA	1761:1785	The histochemical analysis revealed that after 8 weeks, the biodegradation of PLA leads to massive immune cell infusion and inflammation at the site, whereas the PPC composites are well-tolerated in vivo.
26376751	0	37	with	Composite	37:45	arg1	Enhanced					52:59	Enhanced	52:59	Enhanced	52:59	Reinforced Poly(Propylene Carbonate) Composite with Enhanced and Tunable Characteristics, an Alternative for Poly(lactic Acid).
26376751	0	37	with	Composite	37:45	arg1	Characteristics					73:87	Tunable Characteristics	65:87	Tunable Characteristics	65:87	Reinforced Poly(Propylene Carbonate) Composite with Enhanced and Tunable Characteristics, an Alternative for Poly(lactic Acid).
26376751	0	37	with	Composite	37:45	arg1	Alternative					93:103	an Alternative	90:103	an Alternative for Poly(lactic Acid)	90:125	Reinforced Poly(Propylene Carbonate) Composite with Enhanced and Tunable Characteristics, an Alternative for Poly(lactic Acid).
26376751	3	38	theme	degradation	472:482	arg1	dioxide					536:542	carbon dioxide	529:542	carbon dioxide	529:542	The degradation products of PPC-starch composites are mainly carbon dioxide and water; hence, the associated risks to the acidic degradation of polyesters are minimized.
26376751	3	38	theme	degradation	472:482	arg1	products					484:491	The degradation products	468:491	The degradation products of PPC-starch composites	468:516	The degradation products of PPC-starch composites are mainly carbon dioxide and water; hence, the associated risks to the acidic degradation of polyesters are minimized.
26376751	1	39	theme	unpredictable	203:215	arg1	necrosis					249:256	necrosis	249:256	necrosis of host tissues	249:272	The acidic nature of the degradation products of polyesters often leads to unpredictable clinical complications, such as necrosis of host tissues and massive immune cell invasions.
26376751	1	39	theme	unpredictable	203:215	arg1	invasions					298:306	massive immune cell invasions	278:306	massive immune cell invasions	278:306	The acidic nature of the degradation products of polyesters often leads to unpredictable clinical complications, such as necrosis of host tissues and massive immune cell invasions.
26376751	1	39	theme	unpredictable	203:215	arg1	complications					226:238	unpredictable clinical complications	203:238	unpredictable clinical complications	203:238	The acidic nature of the degradation products of polyesters often leads to unpredictable clinical complications, such as necrosis of host tissues and massive immune cell invasions.
26376751	8	40	theme	tissue	1540:1545	arg1	formation					1518:1526	the formation	1514:1526	the formation of fibrotic tissue	1514:1545	In addition, PPC composites in subcutaneous mice model resulted in mild inflammatory responses (e.g., the formation of fibrotic tissue) that were diminished from two to 4 weeks postimplantation.
26376751	10	41	theme	immune	1804:1809	arg1	infusion					1816:1823	massive immune cell infusion	1796:1823	massive immune cell infusion	1796:1823	The histochemical analysis revealed that after 8 weeks, the biodegradation of PLA leads to massive immune cell infusion and inflammation at the site, whereas the PPC composites are well-tolerated in vivo.
26376751	5	42	theme	different	1129:1137	arg1	shapes					1139:1144	different shapes	1129:1144	different shapes	1129:1144	More importantly, the addition of 50 wt % starch enhances the thermal processing capacity of the composites by elevating their decomposition temperature from 245 to 276 °C. Therefore, thermal processing methods, such as extrusion and hot melt compression methods can be used to generate different shapes and structures from PPC-starch composites.
26376751	3	43	theme	composites	507:516	arg1	dioxide					536:542	carbon dioxide	529:542	carbon dioxide	529:542	The degradation products of PPC-starch composites are mainly carbon dioxide and water; hence, the associated risks to the acidic degradation of polyesters are minimized.
26376751	3	43	theme	composites	507:516	arg1	products					484:491	The degradation products	468:491	The degradation products of PPC-starch composites	468:516	The degradation products of PPC-starch composites are mainly carbon dioxide and water; hence, the associated risks to the acidic degradation of polyesters are minimized.
26376751	6	44	dep	cytocompatibility	1214:1230	arg1	the					1210:1212	the	1210:1212	the	1210:1212	We also demonstrated the cytocompatibility and biocompatibility of these composites by conducting in vitro and in vivo tests.
26376751	11	45	theme	biodegradable	2000:2012	arg1	biomaterial					2014:2024	a benign biodegradable biomaterial	1991:2024	a benign biodegradable biomaterial for fabrication of biomedical implants	1991:2063	All these results underline the favorable properties of PPC-starch composites as a benign biodegradable biomaterial for fabrication of biomedical implants.
26376751	11	45	theme	biodegradable	2000:2012	arg1	properties					1952:1961	the favorable properties	1938:1961	the favorable properties of PPC-starch composites	1938:1986	All these results underline the favorable properties of PPC-starch composites as a benign biodegradable biomaterial for fabrication of biomedical implants.
26376751	11	46	theme	implants	2056:2063	arg1	fabrication					2030:2040	fabrication	2030:2040	fabrication of biomedical implants	2030:2063	All these results underline the favorable properties of PPC-starch composites as a benign biodegradable biomaterial for fabrication of biomedical implants.
26376751	4	47	theme	composites	794:803	arg1	contents					782:789	the starch contents	771:789	the starch contents of composites to address different clinical needs	771:839	Moreover, the compression strength of PPC-starch composites can be tuned over the range of 0.2±0.03 MPa to 33.9±1.51 MPa by changing the starch contents of composites to address different clinical needs.
26376751	6	48	theme	in	1300:1301	arg1	tests					1308:1312	in vitro and in vivo tests	1287:1312	in vitro and in vivo tests	1287:1312	We also demonstrated the cytocompatibility and biocompatibility of these composites by conducting in vitro and in vivo tests.
26376751	1	49	theme	products	165:172	arg1	nature					139:144	The acidic nature	128:144	The acidic nature of the degradation products of polyesters	128:186	The acidic nature of the degradation products of polyesters often leads to unpredictable clinical complications, such as necrosis of host tissues and massive immune cell invasions.
26376751	7	50	theme	osteoblast	1344:1353	arg1	cells					1355:1359	osteoblast cells	1344:1359	osteoblast cells	1344:1359	For instance, the numbers of osteoblast cells were increased 2.5 fold after 7 days post culture.
26376751	9	51	theme	long-term	1611:1619	arg1	biodegradation					1629:1642	The long-term in vivo biodegradation	1607:1642	The long-term in vivo biodegradation of PPC composites	1607:1660	The long-term in vivo biodegradation of PPC composites are compared with poly(lactic acid) (PLA).
26376751	10	52	theme	histochemical	1709:1721	arg1	analysis					1723:1730	The histochemical analysis	1705:1730	The histochemical analysis	1705:1730	The histochemical analysis revealed that after 8 weeks, the biodegradation of PLA leads to massive immune cell infusion and inflammation at the site, whereas the PPC composites are well-tolerated in vivo.
26376751	1	53	theme	polyesters	177:186	arg1	products					165:172	the degradation products	149:172	the degradation products of polyesters	149:186	The acidic nature of the degradation products of polyesters often leads to unpredictable clinical complications, such as necrosis of host tissues and massive immune cell invasions.
26376751	6	54	dep	in	1287:1288	arg1	vitro					1290:1294	vitro	1290:1294	vitro	1290:1294	We also demonstrated the cytocompatibility and biocompatibility of these composites by conducting in vitro and in vivo tests.
26376751	11	55	theme	favorable	1942:1950	arg1	properties					1952:1961	the favorable properties	1938:1961	the favorable properties of PPC-starch composites	1938:1986	All these results underline the favorable properties of PPC-starch composites as a benign biodegradable biomaterial for fabrication of biomedical implants.
26376751	11	55	theme	favorable	1942:1950	arg1	biomaterial					2014:2024	a benign biodegradable biomaterial	1991:2024	a benign biodegradable biomaterial for fabrication of biomedical implants	1991:2063	All these results underline the favorable properties of PPC-starch composites as a benign biodegradable biomaterial for fabrication of biomedical implants.
26376751	5	56	from	composites	1177:1186	arg1	structures					1150:1159	structures	1150:1159	structures	1150:1159	More importantly, the addition of 50 wt % starch enhances the thermal processing capacity of the composites by elevating their decomposition temperature from 245 to 276 °C. Therefore, thermal processing methods, such as extrusion and hot melt compression methods can be used to generate different shapes and structures from PPC-starch composites.
26376751	5	56	from	composites	1177:1186	arg1	shapes					1139:1144	different shapes	1129:1144	different shapes	1129:1144	More importantly, the addition of 50 wt % starch enhances the thermal processing capacity of the composites by elevating their decomposition temperature from 245 to 276 °C. Therefore, thermal processing methods, such as extrusion and hot melt compression methods can be used to generate different shapes and structures from PPC-starch composites.
26376751	5	57	theme	thermal	1026:1032	arg1	extrusion					1062:1070	extrusion	1062:1070	extrusion	1062:1070	More importantly, the addition of 50 wt % starch enhances the thermal processing capacity of the composites by elevating their decomposition temperature from 245 to 276 °C. Therefore, thermal processing methods, such as extrusion and hot melt compression methods can be used to generate different shapes and structures from PPC-starch composites.
26376751	5	57	theme	thermal	1026:1032	arg1	methods					1045:1051	thermal processing methods	1026:1051	thermal processing methods	1026:1051	More importantly, the addition of 50 wt % starch enhances the thermal processing capacity of the composites by elevating their decomposition temperature from 245 to 276 °C. Therefore, thermal processing methods, such as extrusion and hot melt compression methods can be used to generate different shapes and structures from PPC-starch composites.
26376751	5	57	theme	thermal	1026:1032	arg1	compression					1085:1095	hot melt compression	1076:1095	hot melt compression	1076:1095	More importantly, the addition of 50 wt % starch enhances the thermal processing capacity of the composites by elevating their decomposition temperature from 245 to 276 °C. Therefore, thermal processing methods, such as extrusion and hot melt compression methods can be used to generate different shapes and structures from PPC-starch composites.
26376751	9	58	theme	composites	1651:1660	arg1	biodegradation					1629:1642	The long-term in vivo biodegradation	1607:1642	The long-term in vivo biodegradation of PPC composites	1607:1660	The long-term in vivo biodegradation of PPC composites are compared with poly(lactic acid) (PLA).
26376751	6	59	theme	composites	1262:1271	arg1	biocompatibility					1236:1251	biocompatibility	1236:1251	biocompatibility	1236:1251	We also demonstrated the cytocompatibility and biocompatibility of these composites by conducting in vitro and in vivo tests.
26376751	6	59	theme	composites	1262:1271	arg1	cytocompatibility					1214:1230	cytocompatibility	1214:1230	cytocompatibility	1214:1230	We also demonstrated the cytocompatibility and biocompatibility of these composites by conducting in vitro and in vivo tests.
26376751	11	60	theme	composites	1977:1986	arg1	properties					1952:1961	the favorable properties	1938:1961	the favorable properties of PPC-starch composites	1938:1986	All these results underline the favorable properties of PPC-starch composites as a benign biodegradable biomaterial for fabrication of biomedical implants.
26376751	11	60	theme	composites	1977:1986	arg1	biomaterial					2014:2024	a benign biodegradable biomaterial	1991:2024	a benign biodegradable biomaterial for fabrication of biomedical implants	1991:2063	All these results underline the favorable properties of PPC-starch composites as a benign biodegradable biomaterial for fabrication of biomedical implants.
26376751	4	61	theme	PPC-starch	676:685	arg1	composites					687:696	PPC-starch composites	676:696	PPC-starch composites	676:696	Moreover, the compression strength of PPC-starch composites can be tuned over the range of 0.2±0.03 MPa to 33.9±1.51 MPa by changing the starch contents of composites to address different clinical needs.
26376751	2	62	theme	polyester-based	442:456	arg1	polymers					458:465	polyester-based polymers	442:465	polyester-based polymers	442:465	In this study, poly(propylene carbonate) (PPC) and starch composite is introduced with superior characteristics as an alternative to polyester-based polymers.
26376751	8	63	theme	fibrotic	1531:1538	arg1	tissue					1540:1545	fibrotic tissue	1531:1545	fibrotic tissue	1531:1545	In addition, PPC composites in subcutaneous mice model resulted in mild inflammatory responses (e.g., the formation of fibrotic tissue) that were diminished from two to 4 weeks postimplantation.
26376751	9	64	dep	in	1621:1622	arg1	vivo					1624:1627	vivo	1624:1627	vivo	1624:1627	The long-term in vivo biodegradation of PPC composites are compared with poly(lactic acid) (PLA).
26376751	10	65	from	site	1849:1852	arg1	inflammation					1829:1840	inflammation	1829:1840	inflammation	1829:1840	The histochemical analysis revealed that after 8 weeks, the biodegradation of PLA leads to massive immune cell infusion and inflammation at the site, whereas the PPC composites are well-tolerated in vivo.
26376751	10	65	from	site	1849:1852	arg1	infusion					1816:1823	massive immune cell infusion	1796:1823	massive immune cell infusion	1796:1823	The histochemical analysis revealed that after 8 weeks, the biodegradation of PLA leads to massive immune cell infusion and inflammation at the site, whereas the PPC composites are well-tolerated in vivo.
26376751	3	66	dep	dioxide	536:542	arg1	minimized					627:635	minimized	627:635	are minimized	623:635	The degradation products of PPC-starch composites are mainly carbon dioxide and water; hence, the associated risks to the acidic degradation of polyesters are minimized.
26376751	9	67	theme	lactic	1685:1690	arg1	poly					1680:1683	poly	1680:1683	poly(lactic acid) (PLA)	1680:1702	The long-term in vivo biodegradation of PPC composites are compared with poly(lactic acid) (PLA).
26376751	9	67	theme	lactic	1685:1690	arg1	acid					1692:1695	lactic acid	1685:1695	lactic acid	1685:1695	The long-term in vivo biodegradation of PPC composites are compared with poly(lactic acid) (PLA).
26376751	8	68	theme	subcutaneous	1443:1454	arg1	model					1461:1465	subcutaneous mice model	1443:1465	subcutaneous mice model	1443:1465	In addition, PPC composites in subcutaneous mice model resulted in mild inflammatory responses (e.g., the formation of fibrotic tissue) that were diminished from two to 4 weeks postimplantation.
26376751	3	69	theme	polyesters	612:621	arg1	degradation					597:607	the acidic degradation	586:607	the acidic degradation of polyesters	586:621	The degradation products of PPC-starch composites are mainly carbon dioxide and water; hence, the associated risks to the acidic degradation of polyesters are minimized.
26376751	5	70	theme	hot	1076:1078	arg1	compression					1085:1095	hot melt compression	1076:1095	hot melt compression	1076:1095	More importantly, the addition of 50 wt % starch enhances the thermal processing capacity of the composites by elevating their decomposition temperature from 245 to 276 °C. Therefore, thermal processing methods, such as extrusion and hot melt compression methods can be used to generate different shapes and structures from PPC-starch composites.
26376751	5	71	theme	processing	912:921	arg1	capacity					923:930	the thermal processing capacity	900:930	the thermal processing capacity of the composites	900:948	More importantly, the addition of 50 wt % starch enhances the thermal processing capacity of the composites by elevating their decomposition temperature from 245 to 276 °C. Therefore, thermal processing methods, such as extrusion and hot melt compression methods can be used to generate different shapes and structures from PPC-starch composites.
26376751	0	72	theme	Poly	11:14	arg1	Composite					37:45	Poly(Propylene Carbonate) Composite	11:45	Poly(Propylene Carbonate) Composite with Enhanced and Tunable Characteristics, an Alternative for Poly(lactic Acid)	11:125	Reinforced Poly(Propylene Carbonate) Composite with Enhanced and Tunable Characteristics, an Alternative for Poly(lactic Acid).
26376751	5	73	theme	wt	879:880	arg1	%					882:882	50 wt %	876:882	50 wt % starch	876:889	More importantly, the addition of 50 wt % starch enhances the thermal processing capacity of the composites by elevating their decomposition temperature from 245 to 276 °C. Therefore, thermal processing methods, such as extrusion and hot melt compression methods can be used to generate different shapes and structures from PPC-starch composites.
26376751	8	74	theme	inflammatory	1484:1495	arg1	responses					1497:1505	mild inflammatory responses	1479:1505	mild inflammatory responses (e.g., the formation of fibrotic tissue) that were diminished from two to 4 weeks postimplantation	1479:1604	In addition, PPC composites in subcutaneous mice model resulted in mild inflammatory responses (e.g., the formation of fibrotic tissue) that were diminished from two to 4 weeks postimplantation.
26376751	2	75	theme	propylene	329:337	arg1	poly					324:327	poly	324:327	poly(propylene carbonate) (PPC)	324:354	In this study, poly(propylene carbonate) (PPC) and starch composite is introduced with superior characteristics as an alternative to polyester-based polymers.
26376751	2	75	theme	propylene	329:337	arg1	carbonate					339:347	propylene carbonate	329:347	propylene carbonate	329:347	In this study, poly(propylene carbonate) (PPC) and starch composite is introduced with superior characteristics as an alternative to polyester-based polymers.
26376751	0	76	theme	Propylene	16:24	arg1	Composite					37:45	Poly(Propylene Carbonate) Composite	11:45	Poly(Propylene Carbonate) Composite with Enhanced and Tunable Characteristics, an Alternative for Poly(lactic Acid)	11:125	Reinforced Poly(Propylene Carbonate) Composite with Enhanced and Tunable Characteristics, an Alternative for Poly(lactic Acid).
26376751	5	77	theme	starch	884:889	arg1	addition					864:871	the addition	860:871	the addition of 50 wt % starch	860:889	More importantly, the addition of 50 wt % starch enhances the thermal processing capacity of the composites by elevating their decomposition temperature from 245 to 276 °C. Therefore, thermal processing methods, such as extrusion and hot melt compression methods can be used to generate different shapes and structures from PPC-starch composites.
26376751	1	78	theme	tissues	266:272	arg1	necrosis					249:256	necrosis	249:256	necrosis of host tissues	249:272	The acidic nature of the degradation products of polyesters often leads to unpredictable clinical complications, such as necrosis of host tissues and massive immune cell invasions.
26376751	1	78	theme	tissues	266:272	arg1	invasions					298:306	massive immune cell invasions	278:306	massive immune cell invasions	278:306	The acidic nature of the degradation products of polyesters often leads to unpredictable clinical complications, such as necrosis of host tissues and massive immune cell invasions.
26376751	1	79	theme	massive	278:284	arg1	invasions					298:306	massive immune cell invasions	278:306	massive immune cell invasions	278:306	The acidic nature of the degradation products of polyesters often leads to unpredictable clinical complications, such as necrosis of host tissues and massive immune cell invasions.
26376751	5	80	theme	decomposition	969:981	arg1	temperature					983:993	their decomposition temperature	963:993	their decomposition temperature	963:993	More importantly, the addition of 50 wt % starch enhances the thermal processing capacity of the composites by elevating their decomposition temperature from 245 to 276 °C. Therefore, thermal processing methods, such as extrusion and hot melt compression methods can be used to generate different shapes and structures from PPC-starch composites.
26376751	2	81	theme	superior	396:403	arg1	characteristics					405:419	superior characteristics	396:419	superior characteristics	396:419	In this study, poly(propylene carbonate) (PPC) and starch composite is introduced with superior characteristics as an alternative to polyester-based polymers.
26376751	1	82	theme	cell	293:296	arg1	invasions					298:306	massive immune cell invasions	278:306	massive immune cell invasions	278:306	The acidic nature of the degradation products of polyesters often leads to unpredictable clinical complications, such as necrosis of host tissues and massive immune cell invasions.
26376751	6	83	theme	in	1287:1288	arg1	tests					1308:1312	in vitro and in vivo tests	1287:1312	in vitro and in vivo tests	1287:1312	We also demonstrated the cytocompatibility and biocompatibility of these composites by conducting in vitro and in vivo tests.
26376751	5	84	used	used	1112:1115	arg2	extrusion					1062:1070	extrusion	1062:1070	extrusion	1062:1070	More importantly, the addition of 50 wt % starch enhances the thermal processing capacity of the composites by elevating their decomposition temperature from 245 to 276 °C. Therefore, thermal processing methods, such as extrusion and hot melt compression methods can be used to generate different shapes and structures from PPC-starch composites.
26376751	5	84	used	used	1112:1115	arg2	compression					1085:1095	hot melt compression	1076:1095	hot melt compression	1076:1095	More importantly, the addition of 50 wt % starch enhances the thermal processing capacity of the composites by elevating their decomposition temperature from 245 to 276 °C. Therefore, thermal processing methods, such as extrusion and hot melt compression methods can be used to generate different shapes and structures from PPC-starch composites.
26376751	5	84	used	used	1112:1115	arg2	methods					1045:1051	thermal processing methods	1026:1051	thermal processing methods	1026:1051	More importantly, the addition of 50 wt % starch enhances the thermal processing capacity of the composites by elevating their decomposition temperature from 245 to 276 °C. Therefore, thermal processing methods, such as extrusion and hot melt compression methods can be used to generate different shapes and structures from PPC-starch composites.
26376751	4	85	theme	MPa	738:740	arg1	range					720:724	the range	716:724	the range of 0.2±0.03 MPa to 33.9±1.51 MPa	716:757	Moreover, the compression strength of PPC-starch composites can be tuned over the range of 0.2±0.03 MPa to 33.9±1.51 MPa by changing the starch contents of composites to address different clinical needs.
26376751	8	86	theme	weeks	1583:1587	arg1	postimplantation					1589:1604	two to 4 weeks postimplantation	1574:1604	two to 4 weeks postimplantation	1574:1604	In addition, PPC composites in subcutaneous mice model resulted in mild inflammatory responses (e.g., the formation of fibrotic tissue) that were diminished from two to 4 weeks postimplantation.
26376751	5	87	theme	PPC-starch	1166:1175	arg1	composites					1177:1186	PPC-starch composites	1166:1186	PPC-starch composites	1166:1186	More importantly, the addition of 50 wt % starch enhances the thermal processing capacity of the composites by elevating their decomposition temperature from 245 to 276 °C. Therefore, thermal processing methods, such as extrusion and hot melt compression methods can be used to generate different shapes and structures from PPC-starch composites.
26376751	5	88	theme	composites	939:948	arg1	capacity					923:930	the thermal processing capacity	900:930	the thermal processing capacity of the composites	900:948	More importantly, the addition of 50 wt % starch enhances the thermal processing capacity of the composites by elevating their decomposition temperature from 245 to 276 °C. Therefore, thermal processing methods, such as extrusion and hot melt compression methods can be used to generate different shapes and structures from PPC-starch composites.
26376751	5	89	dep	°C.	1011:1013	arg1	to					1004:1005	to	1004:1005	to	1004:1005	More importantly, the addition of 50 wt % starch enhances the thermal processing capacity of the composites by elevating their decomposition temperature from 245 to 276 °C. Therefore, thermal processing methods, such as extrusion and hot melt compression methods can be used to generate different shapes and structures from PPC-starch composites.
26376751	5	89	dep	°C.	1011:1013	arg1	used					1112:1115	used	1112:1115	can be used to generate different shapes and structures from PPC-starch composites	1105:1186	More importantly, the addition of 50 wt % starch enhances the thermal processing capacity of the composites by elevating their decomposition temperature from 245 to 276 °C. Therefore, thermal processing methods, such as extrusion and hot melt compression methods can be used to generate different shapes and structures from PPC-starch composites.
26376751	10	90	theme	cell	1811:1814	arg1	infusion					1816:1823	massive immune cell infusion	1796:1823	massive immune cell infusion	1796:1823	The histochemical analysis revealed that after 8 weeks, the biodegradation of PLA leads to massive immune cell infusion and inflammation at the site, whereas the PPC composites are well-tolerated in vivo.
26376751	1	91	theme	clinical	217:224	arg1	necrosis					249:256	necrosis	249:256	necrosis of host tissues	249:272	The acidic nature of the degradation products of polyesters often leads to unpredictable clinical complications, such as necrosis of host tissues and massive immune cell invasions.
26376751	1	91	theme	clinical	217:224	arg1	invasions					298:306	massive immune cell invasions	278:306	massive immune cell invasions	278:306	The acidic nature of the degradation products of polyesters often leads to unpredictable clinical complications, such as necrosis of host tissues and massive immune cell invasions.
26376751	1	91	theme	clinical	217:224	arg1	complications					226:238	unpredictable clinical complications	203:238	unpredictable clinical complications	203:238	The acidic nature of the degradation products of polyesters often leads to unpredictable clinical complications, such as necrosis of host tissues and massive immune cell invasions.
28554014	4	0	theme	amylolytic	800:809	arg1	enzymes					811:817	other amylolytic enzymes	794:817	other amylolytic enzymes	794:817	These analyses showed that the isoamylase could specifically and efficiently attack α-1,6-glucosidic linkages at branch points, leaving the amylose favored by other amylolytic enzymes.
28554014	2	1	theme	debranching	354:364	arg1	enzyme					366:371	a thermostable isoamylase-type debranching enzyme	323:371	a thermostable isoamylase-type debranching enzyme	323:371	In the present study, the action pattern of a thermostable isoamylase-type debranching enzyme on different types of starch was investigated.
28554014	7	2	theme	NU	1194:1195	arg1	amylase					1197:1203	2 NU amylase	1192:1203	2 NU amylase per g starch	1192:1216	The highest RS yield (53.8%) was achieved under the optimized conditions of 70 °C and pH 5.0, using 7 U isoamylase per g starch and 2 NU amylase per g starch.
28554014	7	3	theme	70 °C	1136:1140	arg1	conditions					1122:1131	the optimized conditions	1108:1131	the optimized conditions of 70 °C and pH 5.0	1108:1151	The highest RS yield (53.8%) was achieved under the optimized conditions of 70 °C and pH 5.0, using 7 U isoamylase per g starch and 2 NU amylase per g starch.
28554014	2	4	theme	isoamylase-type	338:352	arg1	enzyme					366:371	a thermostable isoamylase-type debranching enzyme	323:371	a thermostable isoamylase-type debranching enzyme	323:371	In the present study, the action pattern of a thermostable isoamylase-type debranching enzyme on different types of starch was investigated.
28554014	5	5	theme	preparation	971:981	arg1	process					983:989	the RS preparation process	964:989	the RS preparation process	964:989	Its ability to attack side chains composed of 1-3 glucose residues differentiates it from other isoamylases, a property which is also ideal for the RS preparation process.
28554014	8	6	theme	starch	1346:1351	arg1	materials					1353:1361	highly branched starch materials	1330:1361	highly branched starch materials	1330:1361	These data also help us better understand the application of isoamylase for preparation of other products from highly branched starch materials.
28554014	7	7	theme	optimized	1112:1120	arg1	conditions					1122:1131	the optimized conditions	1108:1131	the optimized conditions of 70 °C and pH 5.0	1108:1151	The highest RS yield (53.8%) was achieved under the optimized conditions of 70 °C and pH 5.0, using 7 U isoamylase per g starch and 2 NU amylase per g starch.
28554014	2	8	from	pattern	312:318	arg1	types					386:390	different types	376:390	different types of starch	376:400	In the present study, the action pattern of a thermostable isoamylase-type debranching enzyme on different types of starch was investigated.
28554014	2	9	theme	present	286:292	arg1	study					294:298	the present study	282:298	the present study	282:298	In the present study, the action pattern of a thermostable isoamylase-type debranching enzyme on different types of starch was investigated.
28554014	3	10	theme	magnetic	596:603	arg1	resonance					605:613	nuclear magnetic resonance	588:613	nuclear magnetic resonance spectroscopy (NMR)	588:632	The molecular weight distribution, glycosidic bond composition and contents of oligosaccharides released were monitored by various liquid chromatography techniques and nuclear magnetic resonance spectroscopy (NMR).
28554014	1	11	theme	amylose	264:270	arg1	yield					272:276	amylose yield	264:276	amylose yield	264:276	Debranching enzymes contribute to the enzymatic production of resistant starch (RS) by reducing substrate molecular weight and increasing amylose yield.
28554014	3	12	theme	resonance	605:613	arg1	NMR					629:631	NMR	629:631	NMR	629:631	The molecular weight distribution, glycosidic bond composition and contents of oligosaccharides released were monitored by various liquid chromatography techniques and nuclear magnetic resonance spectroscopy (NMR).
28554014	3	12	theme	resonance	605:613	arg1	spectroscopy					615:626	nuclear magnetic resonance spectroscopy	588:626	nuclear magnetic resonance spectroscopy (NMR)	588:632	The molecular weight distribution, glycosidic bond composition and contents of oligosaccharides released were monitored by various liquid chromatography techniques and nuclear magnetic resonance spectroscopy (NMR).
28554014	7	13	theme	g	1209:1209	arg1	starch					1211:1216	g starch	1209:1216	g starch	1209:1216	The highest RS yield (53.8%) was achieved under the optimized conditions of 70 °C and pH 5.0, using 7 U isoamylase per g starch and 2 NU amylase per g starch.
28554014	5	14	theme	RS	968:969	arg1	process					983:989	the RS preparation process	964:989	the RS preparation process	964:989	Its ability to attack side chains composed of 1-3 glucose residues differentiates it from other isoamylases, a property which is also ideal for the RS preparation process.
28554014	0	15	theme	resistant	108:116	arg1	starch					118:123	resistant starch	108:123	resistant starch	108:123	Investigation of debranching pattern of a thermostable isoamylase and its application for the production of resistant starch.
28554014	3	16	theme	oligosaccharides	499:514	arg1	distribution					441:452	The molecular weight distribution	420:452	The molecular weight distribution	420:452	The molecular weight distribution, glycosidic bond composition and contents of oligosaccharides released were monitored by various liquid chromatography techniques and nuclear magnetic resonance spectroscopy (NMR).
28554014	3	16	theme	oligosaccharides	499:514	arg1	contents					487:494	contents	487:494	contents	487:494	The molecular weight distribution, glycosidic bond composition and contents of oligosaccharides released were monitored by various liquid chromatography techniques and nuclear magnetic resonance spectroscopy (NMR).
28554014	3	16	theme	oligosaccharides	499:514	arg1	composition					471:481	glycosidic bond composition	455:481	glycosidic bond composition	455:481	The molecular weight distribution, glycosidic bond composition and contents of oligosaccharides released were monitored by various liquid chromatography techniques and nuclear magnetic resonance spectroscopy (NMR).
28554014	3	17	theme	liquid	551:556	arg1	techniques					573:582	various liquid chromatography techniques	543:582	various liquid chromatography techniques	543:582	The molecular weight distribution, glycosidic bond composition and contents of oligosaccharides released were monitored by various liquid chromatography techniques and nuclear magnetic resonance spectroscopy (NMR).
28554014	3	18	theme	molecular	424:432	arg1	distribution					441:452	The molecular weight distribution	420:452	The molecular weight distribution	420:452	The molecular weight distribution, glycosidic bond composition and contents of oligosaccharides released were monitored by various liquid chromatography techniques and nuclear magnetic resonance spectroscopy (NMR).
28554014	2	19	theme	starch	395:400	arg1	types					386:390	different types	376:390	different types of starch	376:400	In the present study, the action pattern of a thermostable isoamylase-type debranching enzyme on different types of starch was investigated.
28554014	7	20	theme	g	1179:1179	arg1	starch					1181:1186	g starch	1179:1186	g starch	1179:1186	The highest RS yield (53.8%) was achieved under the optimized conditions of 70 °C and pH 5.0, using 7 U isoamylase per g starch and 2 NU amylase per g starch.
28554014	3	21	theme	weight	434:439	arg1	distribution					441:452	The molecular weight distribution	420:452	The molecular weight distribution	420:452	The molecular weight distribution, glycosidic bond composition and contents of oligosaccharides released were monitored by various liquid chromatography techniques and nuclear magnetic resonance spectroscopy (NMR).
28554014	3	22	theme	chromatography	558:571	arg1	techniques					573:582	various liquid chromatography techniques	543:582	various liquid chromatography techniques	543:582	The molecular weight distribution, glycosidic bond composition and contents of oligosaccharides released were monitored by various liquid chromatography techniques and nuclear magnetic resonance spectroscopy (NMR).
28554014	7	23	theme	U	1162:1162	arg1	isoamylase					1164:1173	7 U isoamylase	1160:1173	7 U isoamylase per g starch	1160:1186	The highest RS yield (53.8%) was achieved under the optimized conditions of 70 °C and pH 5.0, using 7 U isoamylase per g starch and 2 NU amylase per g starch.
28554014	2	24	theme	action	305:310	arg1	pattern					312:318	the action pattern	301:318	the action pattern of a thermostable isoamylase-type debranching enzyme on different types of starch	301:400	In the present study, the action pattern of a thermostable isoamylase-type debranching enzyme on different types of starch was investigated.
28554014	1	25	theme	resistant	188:196	arg1	RS					206:207	RS	206:207	RS	206:207	Debranching enzymes contribute to the enzymatic production of resistant starch (RS) by reducing substrate molecular weight and increasing amylose yield.
28554014	1	25	theme	resistant	188:196	arg1	starch					198:203	resistant starch	188:203	resistant starch (RS)	188:208	Debranching enzymes contribute to the enzymatic production of resistant starch (RS) by reducing substrate molecular weight and increasing amylose yield.
28554014	6	26	from	enzyme	1028:1033	arg1	stage					1053:1057	the hydrolytic stage	1038:1057	the hydrolytic stage	1038:1057	The enzyme was used as an auxiliary enzyme in the hydrolytic stage.
28554014	5	27	theme	other	910:914	arg1	isoamylases					916:926	other isoamylases	910:926	other isoamylases	910:926	Its ability to attack side chains composed of 1-3 glucose residues differentiates it from other isoamylases, a property which is also ideal for the RS preparation process.
28554014	5	27	theme	other	910:914	arg1	property					931:938	a property	929:938	a property which is also ideal for the RS preparation process	929:989	Its ability to attack side chains composed of 1-3 glucose residues differentiates it from other isoamylases, a property which is also ideal for the RS preparation process.
28554014	5	27	theme	other	910:914	arg1	ideal					954:958	ideal	954:958	ideal	954:958	Its ability to attack side chains composed of 1-3 glucose residues differentiates it from other isoamylases, a property which is also ideal for the RS preparation process.
28554014	2	28	theme	different	376:384	arg1	types					386:390	different types	376:390	different types of starch	376:400	In the present study, the action pattern of a thermostable isoamylase-type debranching enzyme on different types of starch was investigated.
28554014	0	29	theme	pattern	29:35	arg1	application					74:84	its application	70:84	its application for the production of resistant starch	70:123	Investigation of debranching pattern of a thermostable isoamylase and its application for the production of resistant starch.
28554014	0	29	theme	pattern	29:35	arg1	Investigation					0:12	Investigation	0:12	Investigation of debranching pattern of a thermostable isoamylase	0:64	Investigation of debranching pattern of a thermostable isoamylase and its application for the production of resistant starch.
28554014	3	30	theme	nuclear	588:594	arg1	resonance					605:613	nuclear magnetic resonance	588:613	nuclear magnetic resonance spectroscopy (NMR)	588:632	The molecular weight distribution, glycosidic bond composition and contents of oligosaccharides released were monitored by various liquid chromatography techniques and nuclear magnetic resonance spectroscopy (NMR).
28554014	0	31	theme	debranching	17:27	arg1	pattern					29:35	debranching pattern	17:35	debranching pattern of a thermostable isoamylase	17:64	Investigation of debranching pattern of a thermostable isoamylase and its application for the production of resistant starch.
28554014	0	32	theme	starch	118:123	arg1	production					94:103	the production	90:103	the production of resistant starch	90:123	Investigation of debranching pattern of a thermostable isoamylase and its application for the production of resistant starch.
28554014	1	33	theme	enzymatic	164:172	arg1	production					174:183	the enzymatic production	160:183	the enzymatic production of resistant starch (RS)	160:208	Debranching enzymes contribute to the enzymatic production of resistant starch (RS) by reducing substrate molecular weight and increasing amylose yield.
28554014	8	34	theme	branched	1337:1344	arg1	materials					1353:1361	highly branched starch materials	1330:1361	highly branched starch materials	1330:1361	These data also help us better understand the application of isoamylase for preparation of other products from highly branched starch materials.
28554014	8	35	theme	products	1316:1323	arg1	preparation					1295:1305	preparation	1295:1305	preparation of other products from highly branched starch materials	1295:1361	These data also help us better understand the application of isoamylase for preparation of other products from highly branched starch materials.
28554014	4	36	theme	α-1,6-glucosidic	719:734	arg1	linkages					736:743	α-1,6-glucosidic linkages	719:743	α-1,6-glucosidic linkages	719:743	These analyses showed that the isoamylase could specifically and efficiently attack α-1,6-glucosidic linkages at branch points, leaving the amylose favored by other amylolytic enzymes.
28554014	1	37	theme	starch	198:203	arg1	production					174:183	the enzymatic production	160:183	the enzymatic production of resistant starch (RS)	160:208	Debranching enzymes contribute to the enzymatic production of resistant starch (RS) by reducing substrate molecular weight and increasing amylose yield.
28554014	3	38	theme	glycosidic	455:464	arg1	composition					471:481	glycosidic bond composition	455:481	glycosidic bond composition	455:481	The molecular weight distribution, glycosidic bond composition and contents of oligosaccharides released were monitored by various liquid chromatography techniques and nuclear magnetic resonance spectroscopy (NMR).
28554014	5	39	theme	attack	835:840	arg1	chains					847:852	attack side chains	835:852	attack side chains composed of 1-3 glucose residues	835:885	Its ability to attack side chains composed of 1-3 glucose residues differentiates it from other isoamylases, a property which is also ideal for the RS preparation process.
28554014	6	40	theme	hydrolytic	1042:1051	arg1	stage					1053:1057	the hydrolytic stage	1038:1057	the hydrolytic stage	1038:1057	The enzyme was used as an auxiliary enzyme in the hydrolytic stage.
28554014	3	41	theme	bond	466:469	arg1	composition					471:481	glycosidic bond composition	455:481	glycosidic bond composition	455:481	The molecular weight distribution, glycosidic bond composition and contents of oligosaccharides released were monitored by various liquid chromatography techniques and nuclear magnetic resonance spectroscopy (NMR).
28554014	0	42	theme	isoamylase	55:64	arg1	pattern					29:35	debranching pattern	17:35	debranching pattern of a thermostable isoamylase	17:64	Investigation of debranching pattern of a thermostable isoamylase and its application for the production of resistant starch.
28554014	7	43	theme	RS	1072:1073	arg1	yield					1075:1079	The highest RS yield	1060:1079	The highest RS yield (53.8%)	1060:1087	The highest RS yield (53.8%) was achieved under the optimized conditions of 70 °C and pH 5.0, using 7 U isoamylase per g starch and 2 NU amylase per g starch.
28554014	7	43	theme	RS	1072:1073	arg1	%					1086:1086	53.8%	1082:1086	53.8%	1082:1086	The highest RS yield (53.8%) was achieved under the optimized conditions of 70 °C and pH 5.0, using 7 U isoamylase per g starch and 2 NU amylase per g starch.
28554014	2	44	theme	thermostable	325:336	arg1	enzyme					366:371	a thermostable isoamylase-type debranching enzyme	323:371	a thermostable isoamylase-type debranching enzyme	323:371	In the present study, the action pattern of a thermostable isoamylase-type debranching enzyme on different types of starch was investigated.
28554014	0	45	theme	thermostable	42:53	arg1	isoamylase					55:64	a thermostable isoamylase	40:64	a thermostable isoamylase	40:64	Investigation of debranching pattern of a thermostable isoamylase and its application for the production of resistant starch.
28554014	6	46	used	used	1007:1010	arg2	enzyme					996:1001	The enzyme	992:1001	The enzyme	992:1001	The enzyme was used as an auxiliary enzyme in the hydrolytic stage.
28554014	6	46	used	used	1007:1010	arg2	enzyme					1028:1033	an auxiliary enzyme	1015:1033	an auxiliary enzyme in the hydrolytic stage	1015:1057	The enzyme was used as an auxiliary enzyme in the hydrolytic stage.
28554014	7	47	theme	pH	1146:1147	arg1	conditions					1122:1131	the optimized conditions	1108:1131	the optimized conditions of 70 °C and pH 5.0	1108:1151	The highest RS yield (53.8%) was achieved under the optimized conditions of 70 °C and pH 5.0, using 7 U isoamylase per g starch and 2 NU amylase per g starch.
28554014	1	48	theme	Debranching	126:136	arg1	enzymes					138:144	Debranching enzymes	126:144	Debranching enzymes	126:144	Debranching enzymes contribute to the enzymatic production of resistant starch (RS) by reducing substrate molecular weight and increasing amylose yield.
28554014	4	49	theme	branch	748:753	arg1	points					755:760	branch points	748:760	branch points	748:760	These analyses showed that the isoamylase could specifically and efficiently attack α-1,6-glucosidic linkages at branch points, leaving the amylose favored by other amylolytic enzymes.
28554014	6	50	theme	auxiliary	1018:1026	arg1	enzyme					1028:1033	an auxiliary enzyme	1015:1033	an auxiliary enzyme in the hydrolytic stage	1015:1057	The enzyme was used as an auxiliary enzyme in the hydrolytic stage.
28554014	6	50	theme	auxiliary	1018:1026	arg1	enzyme					996:1001	The enzyme	992:1001	The enzyme	992:1001	The enzyme was used as an auxiliary enzyme in the hydrolytic stage.
28554014	7	51	theme	highest	1064:1070	arg1	yield					1075:1079	The highest RS yield	1060:1079	The highest RS yield (53.8%)	1060:1087	The highest RS yield (53.8%) was achieved under the optimized conditions of 70 °C and pH 5.0, using 7 U isoamylase per g starch and 2 NU amylase per g starch.
28554014	7	51	theme	highest	1064:1070	arg1	%					1086:1086	53.8%	1082:1086	53.8%	1082:1086	The highest RS yield (53.8%) was achieved under the optimized conditions of 70 °C and pH 5.0, using 7 U isoamylase per g starch and 2 NU amylase per g starch.
28554014	8	52	theme	other	1310:1314	arg1	products					1316:1323	other products	1310:1323	other products	1310:1323	These data also help us better understand the application of isoamylase for preparation of other products from highly branched starch materials.
28554014	4	53	theme	other	794:798	arg1	enzymes					811:817	other amylolytic enzymes	794:817	other amylolytic enzymes	794:817	These analyses showed that the isoamylase could specifically and efficiently attack α-1,6-glucosidic linkages at branch points, leaving the amylose favored by other amylolytic enzymes.
28554014	3	54	theme	various	543:549	arg1	techniques					573:582	various liquid chromatography techniques	543:582	various liquid chromatography techniques	543:582	The molecular weight distribution, glycosidic bond composition and contents of oligosaccharides released were monitored by various liquid chromatography techniques and nuclear magnetic resonance spectroscopy (NMR).
28554014	1	55	theme	substrate	222:230	arg1	weight					242:247	substrate molecular weight	222:247	substrate molecular weight	222:247	Debranching enzymes contribute to the enzymatic production of resistant starch (RS) by reducing substrate molecular weight and increasing amylose yield.
28554014	8	56	from	materials	1353:1361	arg1	preparation					1295:1305	preparation	1295:1305	preparation of other products from highly branched starch materials	1295:1361	These data also help us better understand the application of isoamylase for preparation of other products from highly branched starch materials.
28554014	8	57	theme	isoamylase	1280:1289	arg1	application					1265:1275	the application	1261:1275	the application of isoamylase for preparation of other products from highly branched starch materials	1261:1361	These data also help us better understand the application of isoamylase for preparation of other products from highly branched starch materials.
28554014	1	58	theme	molecular	232:240	arg1	weight					242:247	substrate molecular weight	222:247	substrate molecular weight	222:247	Debranching enzymes contribute to the enzymatic production of resistant starch (RS) by reducing substrate molecular weight and increasing amylose yield.
28554014	5	59	theme	side	842:845	arg1	chains					847:852	attack side chains	835:852	attack side chains composed of 1-3 glucose residues	835:885	Its ability to attack side chains composed of 1-3 glucose residues differentiates it from other isoamylases, a property which is also ideal for the RS preparation process.
28554014	5	60	theme	glucose	870:876	arg1	residues					878:885	1-3 glucose residues	866:885	1-3 glucose residues	866:885	Its ability to attack side chains composed of 1-3 glucose residues differentiates it from other isoamylases, a property which is also ideal for the RS preparation process.
28554014	2	61	theme	enzyme	366:371	arg1	pattern					312:318	the action pattern	301:318	the action pattern of a thermostable isoamylase-type debranching enzyme on different types of starch	301:400	In the present study, the action pattern of a thermostable isoamylase-type debranching enzyme on different types of starch was investigated.
28554014	4	62	from	points	755:760	arg1	attack					712:717	attack	712:717	attack	712:717	These analyses showed that the isoamylase could specifically and efficiently attack α-1,6-glucosidic linkages at branch points, leaving the amylose favored by other amylolytic enzymes.
26028419	0	0	theme	tablets	79:85	arg1	disintegration					29:42	disintegration	29:42	disintegration	29:42	[Effect of food thickener on disintegration and dissolution of magnesium oxide tablets].
26028419	0	0	theme	tablets	79:85	arg1	dissolution					48:58	dissolution	48:58	dissolution	48:58	[Effect of food thickener on disintegration and dissolution of magnesium oxide tablets].
26028419	2	1	theme	food	327:330	arg1	thickener					332:340	a food thickener	325:340	a food thickener	325:340	Therefore we examined whether immersion in a food thickener affects the pharmacological effect in patients taking magnesium oxide tablets, and whether immersion affects its disintegration and solubility.
26028419	6	2	theme	available	995:1003	arg1	thickeners					1010:1019	commercially available food thickeners	982:1019	commercially available food thickeners	982:1019	This suggests that commercially available food thickeners are likely to be associated with changes in the degradation of magnesium oxide tablets, and they therefore should be carefully used in certain clinical situations.
26028419	0	3	theme	oxide	73:77	arg1	tablets					79:85	magnesium oxide tablets	63:85	magnesium oxide tablets	63:85	[Effect of food thickener on disintegration and dissolution of magnesium oxide tablets].
26028419	5	4	theme	composition	852:862	arg1	concentrations					864:877	composition concentrations	852:877	composition concentrations	852:877	Furthermore, components that constitute the food thickener and differences in composition concentrations differentially affect the disintegration and solubility of magnesium oxide tablets.
26028419	6	5	theme	magnesium	1084:1092	arg1	tablets					1100:1106	magnesium oxide tablets	1084:1106	magnesium oxide tablets	1084:1106	This suggests that commercially available food thickeners are likely to be associated with changes in the degradation of magnesium oxide tablets, and they therefore should be carefully used in certain clinical situations.
26028419	6	6	from	changes	1054:1060	arg1	degradation					1069:1079	the degradation	1065:1079	the degradation of magnesium oxide tablets	1065:1106	This suggests that commercially available food thickeners are likely to be associated with changes in the degradation of magnesium oxide tablets, and they therefore should be carefully used in certain clinical situations.
26028419	5	7	theme	oxide	948:952	arg1	tablets					954:960	magnesium oxide tablets	938:960	magnesium oxide tablets	938:960	Furthermore, components that constitute the food thickener and differences in composition concentrations differentially affect the disintegration and solubility of magnesium oxide tablets.
26028419	5	8	theme	tablets	954:960	arg1	solubility					924:933	solubility	924:933	solubility	924:933	Furthermore, components that constitute the food thickener and differences in composition concentrations differentially affect the disintegration and solubility of magnesium oxide tablets.
26028419	5	8	theme	tablets	954:960	arg1	disintegration					905:918	disintegration	905:918	disintegration	905:918	Furthermore, components that constitute the food thickener and differences in composition concentrations differentially affect the disintegration and solubility of magnesium oxide tablets.
26028419	5	9	from	differences	837:847	arg1	concentrations					864:877	composition concentrations	852:877	composition concentrations	852:877	Furthermore, components that constitute the food thickener and differences in composition concentrations differentially affect the disintegration and solubility of magnesium oxide tablets.
26028419	4	10	theme	tablets	710:716	arg1	time					668:671	disintegration time	653:671	disintegration time	653:671	The disintegration time and dissolution rate of the immersed tablets were lower than those of non-immersed tablets in vitro.
26028419	4	10	theme	tablets	710:716	arg1	rate					689:692	dissolution rate	677:692	dissolution rate	677:692	The disintegration time and dissolution rate of the immersed tablets were lower than those of non-immersed tablets in vitro.
26028419	2	11	from	immersion	312:320	arg1	thickener					332:340	a food thickener	325:340	a food thickener	325:340	Therefore we examined whether immersion in a food thickener affects the pharmacological effect in patients taking magnesium oxide tablets, and whether immersion affects its disintegration and solubility.
26028419	6	12	theme	certain	1156:1162	arg1	situations					1173:1182	certain clinical situations	1156:1182	certain clinical situations	1156:1182	This suggests that commercially available food thickeners are likely to be associated with changes in the degradation of magnesium oxide tablets, and they therefore should be carefully used in certain clinical situations.
26028419	1	13	theme	non-disintegrated	157:173	arg1	state					175:179	a non-disintegrated state	155:179	a non-disintegrated state	155:179	It has been reported that magnesium oxide tablets are excreted in a non-disintegrated state in the stool of patients when the tablets are administered after being immersed in a food thickener.
26028419	5	14	theme	food	818:821	arg1	thickener					823:831	the food thickener	814:831	the food thickener	814:831	Furthermore, components that constitute the food thickener and differences in composition concentrations differentially affect the disintegration and solubility of magnesium oxide tablets.
26028419	6	15	used	used	1148:1151	arg2	they					1113:1116	they	1113:1116	they	1113:1116	This suggests that commercially available food thickeners are likely to be associated with changes in the degradation of magnesium oxide tablets, and they therefore should be carefully used in certain clinical situations.
26028419	2	16	theme	pharmacological	354:368	arg1	effect					370:375	the pharmacological effect	350:375	the pharmacological effect in patients taking magnesium oxide tablets	350:418	Therefore we examined whether immersion in a food thickener affects the pharmacological effect in patients taking magnesium oxide tablets, and whether immersion affects its disintegration and solubility.
26028419	0	17	theme	thickener	16:24	arg1	[Effect					0:6	[Effect	0:6	[Effect	0:6	[Effect of food thickener on disintegration and dissolution of magnesium oxide tablets].
26028419	4	18	theme	immersed	701:708	arg1	tablets					710:716	the immersed tablets	697:716	the immersed tablets	697:716	The disintegration time and dissolution rate of the immersed tablets were lower than those of non-immersed tablets in vitro.
26028419	2	19	theme	oxide	406:410	arg1	tablets					412:418	magnesium oxide tablets	396:418	magnesium oxide tablets	396:418	Therefore we examined whether immersion in a food thickener affects the pharmacological effect in patients taking magnesium oxide tablets, and whether immersion affects its disintegration and solubility.
26028419	0	20	theme	food	11:14	arg1	thickener					16:24	food thickener	11:24	food thickener	11:24	[Effect of food thickener on disintegration and dissolution of magnesium oxide tablets].
26028419	5	21	theme	magnesium	938:946	arg1	tablets					954:960	magnesium oxide tablets	938:960	magnesium oxide tablets	938:960	Furthermore, components that constitute the food thickener and differences in composition concentrations differentially affect the disintegration and solubility of magnesium oxide tablets.
26028419	2	22	theme	magnesium	396:404	arg1	tablets					412:418	magnesium oxide tablets	396:418	magnesium oxide tablets	396:418	Therefore we examined whether immersion in a food thickener affects the pharmacological effect in patients taking magnesium oxide tablets, and whether immersion affects its disintegration and solubility.
26028419	3	23	theme	mean	490:493	arg1	dosage					495:500	The mean dosage	486:500	The mean dosage (1705 mg/d)	486:512	The mean dosage (1705 mg/d) was higher for patients who took tablets after immersion in a food thickener than for those who took non-immersed tablets (1380 mg/d).
26028419	3	23	theme	mean	490:493	arg1	mg/d					508:511	1705 mg/d	503:511	1705 mg/d	503:511	The mean dosage (1705 mg/d) was higher for patients who took tablets after immersion in a food thickener than for those who took non-immersed tablets (1380 mg/d).
26028419	3	23	theme	mean	490:493	arg1	higher					518:523	higher	518:523	higher	518:523	The mean dosage (1705 mg/d) was higher for patients who took tablets after immersion in a food thickener than for those who took non-immersed tablets (1380 mg/d).
26028419	1	24	theme	food	266:269	arg1	thickener					271:279	a food thickener	264:279	a food thickener	264:279	It has been reported that magnesium oxide tablets are excreted in a non-disintegrated state in the stool of patients when the tablets are administered after being immersed in a food thickener.
26028419	6	25	theme	tablets	1100:1106	arg1	degradation					1069:1079	the degradation	1065:1079	the degradation of magnesium oxide tablets	1065:1106	This suggests that commercially available food thickeners are likely to be associated with changes in the degradation of magnesium oxide tablets, and they therefore should be carefully used in certain clinical situations.
26028419	6	26	theme	clinical	1164:1171	arg1	situations					1173:1182	certain clinical situations	1156:1182	certain clinical situations	1156:1182	This suggests that commercially available food thickeners are likely to be associated with changes in the degradation of magnesium oxide tablets, and they therefore should be carefully used in certain clinical situations.
26028419	4	27	theme	disintegration	653:666	arg1	time					668:671	disintegration time	653:671	disintegration time	653:671	The disintegration time and dissolution rate of the immersed tablets were lower than those of non-immersed tablets in vitro.
26028419	4	28	theme	non-immersed	743:754	arg1	tablets					756:762	non-immersed tablets	743:762	non-immersed tablets	743:762	The disintegration time and dissolution rate of the immersed tablets were lower than those of non-immersed tablets in vitro.
26028419	2	29	from	effect	370:375	arg1	patients					380:387	patients	380:387	patients taking magnesium oxide tablets	380:418	Therefore we examined whether immersion in a food thickener affects the pharmacological effect in patients taking magnesium oxide tablets, and whether immersion affects its disintegration and solubility.
26028419	4	30	theme	dissolution	677:687	arg1	rate					689:692	dissolution rate	677:692	dissolution rate	677:692	The disintegration time and dissolution rate of the immersed tablets were lower than those of non-immersed tablets in vitro.
26028419	4	31	dep	time	668:671	arg1	The					649:651	The	649:651	The	649:651	The disintegration time and dissolution rate of the immersed tablets were lower than those of non-immersed tablets in vitro.
26028419	1	32	theme	patients	197:204	arg1	stool					188:192	the stool	184:192	the stool of patients	184:204	It has been reported that magnesium oxide tablets are excreted in a non-disintegrated state in the stool of patients when the tablets are administered after being immersed in a food thickener.
26028419	3	33	theme	non-immersed	615:626	arg1	tablets					628:634	non-immersed tablets	615:634	non-immersed tablets (1380 mg/d)	615:646	The mean dosage (1705 mg/d) was higher for patients who took tablets after immersion in a food thickener than for those who took non-immersed tablets (1380 mg/d).
26028419	3	33	theme	non-immersed	615:626	arg1	mg/d					642:645	1380 mg/d	637:645	1380 mg/d	637:645	The mean dosage (1705 mg/d) was higher for patients who took tablets after immersion in a food thickener than for those who took non-immersed tablets (1380 mg/d).
26028419	5	34	from	thickener	823:831	arg1	concentrations					864:877	composition concentrations	852:877	composition concentrations	852:877	Furthermore, components that constitute the food thickener and differences in composition concentrations differentially affect the disintegration and solubility of magnesium oxide tablets.
26028419	1	35	theme	magnesium	115:123	arg1	tablets					131:137	magnesium oxide tablets	115:137	magnesium oxide tablets	115:137	It has been reported that magnesium oxide tablets are excreted in a non-disintegrated state in the stool of patients when the tablets are administered after being immersed in a food thickener.
26028419	5	36	dep	disintegration	905:918	arg1	the					901:903	the	901:903	the	901:903	Furthermore, components that constitute the food thickener and differences in composition concentrations differentially affect the disintegration and solubility of magnesium oxide tablets.
26028419	0	37	theme	magnesium	63:71	arg1	tablets					79:85	magnesium oxide tablets	63:85	magnesium oxide tablets	63:85	[Effect of food thickener on disintegration and dissolution of magnesium oxide tablets].
26028419	1	38	theme	oxide	125:129	arg1	tablets					131:137	magnesium oxide tablets	115:137	magnesium oxide tablets	115:137	It has been reported that magnesium oxide tablets are excreted in a non-disintegrated state in the stool of patients when the tablets are administered after being immersed in a food thickener.
26028419	3	39	theme	food	576:579	arg1	thickener					581:589	a food thickener	574:589	a food thickener	574:589	The mean dosage (1705 mg/d) was higher for patients who took tablets after immersion in a food thickener than for those who took non-immersed tablets (1380 mg/d).
26028419	6	40	theme	oxide	1094:1098	arg1	tablets					1100:1106	magnesium oxide tablets	1084:1106	magnesium oxide tablets	1084:1106	This suggests that commercially available food thickeners are likely to be associated with changes in the degradation of magnesium oxide tablets, and they therefore should be carefully used in certain clinical situations.
26028419	6	41	theme	food	1005:1008	arg1	thickeners					1010:1019	commercially available food thickeners	982:1019	commercially available food thickeners	982:1019	This suggests that commercially available food thickeners are likely to be associated with changes in the degradation of magnesium oxide tablets, and they therefore should be carefully used in certain clinical situations.
26566837	0	0	theme	fruit	72:76	arg1	softening					78:86	delay fruit softening	66:86	delay fruit softening	66:86	Induced mutations in tomato SlExp1 alter cell wall metabolism and delay fruit softening.
26566837	4	1	theme	shelf	443:447	arg1	life					449:452	long shelf life	438:452	long shelf life	438:452	To engineer tomato plants with long shelf life, we screened for mutant plants impaired in SlExp1 function.
26566837	1	2	theme	many	137:140	arg1	fruit					149:153	many fleshy fruit	137:153	many fleshy fruit	137:153	Fruit ripening and softening are key traits for many fleshy fruit.
26566837	9	3	from	fruits	1432:1437	arg1	understanding					1358:1370	the understanding	1354:1370	the understanding of the cell wall polysaccharide assembly dynamics in fleshy fruits	1354:1437	These SlExp1 mutants represent good tools for breeding long shelf life tomato lines with contrasted fruit texture as well as for the understanding of the cell wall polysaccharide assembly dynamics in fleshy fruits.
26566837	0	4	theme	delay	66:70	arg1	softening					78:86	delay fruit softening	66:86	delay fruit softening	66:86	Induced mutations in tomato SlExp1 alter cell wall metabolism and delay fruit softening.
26566837	0	5	from	mutations	8:16	arg1	SlExp1					28:33	tomato SlExp1	21:33	tomato SlExp1	21:33	Induced mutations in tomato SlExp1 alter cell wall metabolism and delay fruit softening.
26566837	9	6	theme	good	1256:1259	arg1	tools					1261:1265	good tools	1256:1265	good tools for breeding long shelf life tomato lines with contrasted fruit texture as well as for the understanding of the cell wall polysaccharide assembly dynamics in fleshy fruits	1256:1437	These SlExp1 mutants represent good tools for breeding long shelf life tomato lines with contrasted fruit texture as well as for the understanding of the cell wall polysaccharide assembly dynamics in fleshy fruits.
26566837	5	7	theme	induced	538:544	arg1	Slexp1-7_Q213Stop					577:593	Slexp1-7_Q213Stop	577:593	Slexp1-7_Q213Stop	577:593	Characterization of two induced mutations, Slexp1-6_W211S, and Slexp1-7_Q213Stop, showed that SlExp1 loss of function leads to enhanced fruit firmness and delayed fruit ripening.
26566837	5	7	theme	induced	538:544	arg1	mutations					546:554	two induced mutations	534:554	two induced mutations	534:554	Characterization of two induced mutations, Slexp1-6_W211S, and Slexp1-7_Q213Stop, showed that SlExp1 loss of function leads to enhanced fruit firmness and delayed fruit ripening.
26566837	5	7	theme	induced	538:544	arg1	Slexp1-6_W211S					557:570	Slexp1-6_W211S	557:570	Slexp1-6_W211S	557:570	Characterization of two induced mutations, Slexp1-6_W211S, and Slexp1-7_Q213Stop, showed that SlExp1 loss of function leads to enhanced fruit firmness and delayed fruit ripening.
26566837	1	8	theme	fleshy	142:147	arg1	fruit					149:153	many fleshy fruit	137:153	many fleshy fruit	137:153	Fruit ripening and softening are key traits for many fleshy fruit.
26566837	5	9	theme	delayed	669:675	arg1	ripening					683:690	delayed fruit ripening	669:690	delayed fruit ripening	669:690	Characterization of two induced mutations, Slexp1-6_W211S, and Slexp1-7_Q213Stop, showed that SlExp1 loss of function leads to enhanced fruit firmness and delayed fruit ripening.
26566837	5	10	theme	SlExp1	608:613	arg1	loss					615:618	SlExp1 loss	608:618	SlExp1 loss of function	608:630	Characterization of two induced mutations, Slexp1-6_W211S, and Slexp1-7_Q213Stop, showed that SlExp1 loss of function leads to enhanced fruit firmness and delayed fruit ripening.
26566837	5	11	theme	mutations	546:554	arg1	Characterization					514:529	Characterization	514:529	Characterization of two induced mutations, Slexp1-6_W211S, and Slexp1-7_Q213Stop,	514:594	Characterization of two induced mutations, Slexp1-6_W211S, and Slexp1-7_Q213Stop, showed that SlExp1 loss of function leads to enhanced fruit firmness and delayed fruit ripening.
26566837	3	12	theme	Expansin	323:330	arg1	SlExp1					340:345	SlExp1	340:345	SlExp1	340:345	In tomato, expression of Expansin 1 gene, SlExp1, during fruit ripening was associated with fruit softening.
26566837	3	12	theme	Expansin	323:330	arg1	gene					334:337	Expansin 1 gene	323:337	Expansin 1 gene	323:337	In tomato, expression of Expansin 1 gene, SlExp1, during fruit ripening was associated with fruit softening.
26566837	6	13	theme	cell	705:708	arg1	composition					730:740	cell wall polysaccharide composition	705:740	cell wall polysaccharide composition of Slexp1-7_Q213Stop mutant	705:768	Analysis of cell wall polysaccharide composition of Slexp1-7_Q213Stop mutant pointed out significant differences for uronic acid, neutral sugar and total sugar contents.
26566837	4	14	with	plants	426:431	arg1	life					449:452	long shelf life	438:452	long shelf life	438:452	To engineer tomato plants with long shelf life, we screened for mutant plants impaired in SlExp1 function.
26566837	9	15	with	lines	1303:1307	arg1	texture					1331:1337	contrasted fruit texture	1314:1337	contrasted fruit texture	1314:1337	These SlExp1 mutants represent good tools for breeding long shelf life tomato lines with contrasted fruit texture as well as for the understanding of the cell wall polysaccharide assembly dynamics in fleshy fruits.
26566837	8	16	theme	hemicellulose	1200:1212	arg1	structure					1214:1222	hemicellulose structure	1200:1222	hemicellulose structure	1200:1222	Altogether, these results demonstrated that SlExp1 loss of function mutants yield firmer and late ripening fruits through modification of hemicellulose structure.
26566837	4	17	theme	tomato	419:424	arg1	plants					426:431	tomato plants	419:431	tomato plants with long shelf life	419:452	To engineer tomato plants with long shelf life, we screened for mutant plants impaired in SlExp1 function.
26566837	6	18	theme	mutant	763:768	arg1	composition					730:740	cell wall polysaccharide composition	705:740	cell wall polysaccharide composition of Slexp1-7_Q213Stop mutant	705:768	Analysis of cell wall polysaccharide composition of Slexp1-7_Q213Stop mutant pointed out significant differences for uronic acid, neutral sugar and total sugar contents.
26566837	4	19	theme	long	438:441	arg1	life					449:452	long shelf life	438:452	long shelf life	438:452	To engineer tomato plants with long shelf life, we screened for mutant plants impaired in SlExp1 function.
26566837	6	20	theme	Slexp1-7_Q213Stop	745:761	arg1	mutant					763:768	Slexp1-7_Q213Stop mutant	745:768	Slexp1-7_Q213Stop mutant	745:768	Analysis of cell wall polysaccharide composition of Slexp1-7_Q213Stop mutant pointed out significant differences for uronic acid, neutral sugar and total sugar contents.
26566837	8	21	theme	structure	1214:1222	arg1	modification					1184:1195	modification	1184:1195	modification of hemicellulose structure	1184:1222	Altogether, these results demonstrated that SlExp1 loss of function mutants yield firmer and late ripening fruits through modification of hemicellulose structure.
26566837	3	22	theme	fruit	390:394	arg1	softening					396:404	fruit softening	390:404	fruit softening	390:404	In tomato, expression of Expansin 1 gene, SlExp1, during fruit ripening was associated with fruit softening.
26566837	6	23	theme	total	841:845	arg1	contents					853:860	total sugar contents	841:860	total sugar contents	841:860	Analysis of cell wall polysaccharide composition of Slexp1-7_Q213Stop mutant pointed out significant differences for uronic acid, neutral sugar and total sugar contents.
26566837	9	24	from	understanding	1358:1370	arg1	fruits					1432:1437	fleshy fruits	1425:1437	fleshy fruits	1425:1437	These SlExp1 mutants represent good tools for breeding long shelf life tomato lines with contrasted fruit texture as well as for the understanding of the cell wall polysaccharide assembly dynamics in fleshy fruits.
26566837	7	25	theme	MALDI-TOF	938:946	arg1	spectrometry					948:959	MALDI-TOF spectrometry	938:959	MALDI-TOF spectrometry	938:959	Hemicelluloses chemistry analysis by endo-β-1,4-d-glucanase hydrolysis and MALDI-TOF spectrometry revealed that xyloglucan structures were affected in the fruit pericarp of Slexp1-7_Q213Stop mutant.
26566837	8	26	theme	SlExp1	1106:1111	arg1	loss					1113:1116	SlExp1 loss	1106:1116	SlExp1 loss of function mutants	1106:1136	Altogether, these results demonstrated that SlExp1 loss of function mutants yield firmer and late ripening fruits through modification of hemicellulose structure.
26566837	0	27	theme	Induced	0:6	arg1	mutations					8:16	Induced mutations	0:16	Induced mutations in tomato SlExp1	0:33	Induced mutations in tomato SlExp1 alter cell wall metabolism and delay fruit softening.
26566837	8	28	theme	function	1121:1128	arg1	mutants					1130:1136	function mutants	1121:1136	function mutants	1121:1136	Altogether, these results demonstrated that SlExp1 loss of function mutants yield firmer and late ripening fruits through modification of hemicellulose structure.
26566837	6	29	theme	composition	730:740	arg1	Analysis					693:700	Analysis	693:700	Analysis of cell wall polysaccharide composition of Slexp1-7_Q213Stop mutant	693:768	Analysis of cell wall polysaccharide composition of Slexp1-7_Q213Stop mutant pointed out significant differences for uronic acid, neutral sugar and total sugar contents.
26566837	9	30	theme	life	1291:1294	arg1	lines					1303:1307	breeding long shelf life tomato lines	1271:1307	breeding long shelf life tomato lines with contrasted fruit texture as well as for the understanding of the cell wall polysaccharide assembly dynamics in fleshy fruits	1271:1437	These SlExp1 mutants represent good tools for breeding long shelf life tomato lines with contrasted fruit texture as well as for the understanding of the cell wall polysaccharide assembly dynamics in fleshy fruits.
26566837	9	31	from	dynamics	1413:1420	arg1	fruits					1432:1437	fleshy fruits	1425:1437	fleshy fruits	1425:1437	These SlExp1 mutants represent good tools for breeding long shelf life tomato lines with contrasted fruit texture as well as for the understanding of the cell wall polysaccharide assembly dynamics in fleshy fruits.
26566837	0	32	theme	tomato	21:26	arg1	SlExp1					28:33	tomato SlExp1	21:33	tomato SlExp1	21:33	Induced mutations in tomato SlExp1 alter cell wall metabolism and delay fruit softening.
26566837	7	33	theme	endo-β-1,4-d-glucanase	900:921	arg1	hydrolysis					923:932	endo-β-1,4-d-glucanase hydrolysis	900:932	endo-β-1,4-d-glucanase hydrolysis	900:932	Hemicelluloses chemistry analysis by endo-β-1,4-d-glucanase hydrolysis and MALDI-TOF spectrometry revealed that xyloglucan structures were affected in the fruit pericarp of Slexp1-7_Q213Stop mutant.
26566837	5	34	theme	enhanced	641:648	arg1	firmness					656:663	enhanced fruit firmness	641:663	enhanced fruit firmness	641:663	Characterization of two induced mutations, Slexp1-6_W211S, and Slexp1-7_Q213Stop, showed that SlExp1 loss of function leads to enhanced fruit firmness and delayed fruit ripening.
26566837	6	35	theme	polysaccharide	715:728	arg1	composition					730:740	cell wall polysaccharide composition	705:740	cell wall polysaccharide composition of Slexp1-7_Q213Stop mutant	705:768	Analysis of cell wall polysaccharide composition of Slexp1-7_Q213Stop mutant pointed out significant differences for uronic acid, neutral sugar and total sugar contents.
26566837	1	36	theme	Fruit	89:93	arg1	softening					108:116	softening	108:116	softening	108:116	Fruit ripening and softening are key traits for many fleshy fruit.
26566837	1	36	theme	Fruit	89:93	arg1	traits					126:131	key traits	122:131	key traits for many fleshy fruit	122:153	Fruit ripening and softening are key traits for many fleshy fruit.
26566837	1	36	theme	Fruit	89:93	arg1	ripening					95:102	Fruit ripening	89:102	Fruit ripening	89:102	Fruit ripening and softening are key traits for many fleshy fruit.
26566837	6	37	theme	significant	782:792	arg1	differences					794:804	significant differences	782:804	significant differences for uronic acid, neutral sugar and total sugar contents	782:860	Analysis of cell wall polysaccharide composition of Slexp1-7_Q213Stop mutant pointed out significant differences for uronic acid, neutral sugar and total sugar contents.
26566837	3	38	theme	gene	334:337	arg1	expression					309:318	expression	309:318	expression of Expansin 1 gene, SlExp1, during fruit ripening	309:368	In tomato, expression of Expansin 1 gene, SlExp1, during fruit ripening was associated with fruit softening.
26566837	5	39	theme	fruit	650:654	arg1	firmness					656:663	enhanced fruit firmness	641:663	enhanced fruit firmness	641:663	Characterization of two induced mutations, Slexp1-6_W211S, and Slexp1-7_Q213Stop, showed that SlExp1 loss of function leads to enhanced fruit firmness and delayed fruit ripening.
26566837	2	40	theme	cell	162:165	arg1	walls					167:171	cell walls	162:171	cell walls	162:171	Since cell walls play a key role in the softening process, expansins have been investigated to control fruit over ripening and deterioration.
26566837	8	41	theme	ripening	1160:1167	arg1	fruits					1169:1174	firmer and late ripening fruits	1144:1174	firmer and late ripening fruits	1144:1174	Altogether, these results demonstrated that SlExp1 loss of function mutants yield firmer and late ripening fruits through modification of hemicellulose structure.
26566837	3	42	theme	fruit	355:359	arg1	ripening					361:368	fruit ripening	355:368	fruit ripening	355:368	In tomato, expression of Expansin 1 gene, SlExp1, during fruit ripening was associated with fruit softening.
26566837	4	43	theme	SlExp1	497:502	arg1	function					504:511	SlExp1 function	497:511	SlExp1 function	497:511	To engineer tomato plants with long shelf life, we screened for mutant plants impaired in SlExp1 function.
26566837	9	44	theme	breeding	1271:1278	arg1	lines					1303:1307	breeding long shelf life tomato lines	1271:1307	breeding long shelf life tomato lines with contrasted fruit texture as well as for the understanding of the cell wall polysaccharide assembly dynamics in fleshy fruits	1271:1437	These SlExp1 mutants represent good tools for breeding long shelf life tomato lines with contrasted fruit texture as well as for the understanding of the cell wall polysaccharide assembly dynamics in fleshy fruits.
26566837	2	45	theme	softening	196:204	arg1	process					206:212	the softening process	192:212	the softening process	192:212	Since cell walls play a key role in the softening process, expansins have been investigated to control fruit over ripening and deterioration.
26566837	8	46	theme	firmer	1144:1149	arg1	fruits					1169:1174	firmer and late ripening fruits	1144:1174	firmer and late ripening fruits	1144:1174	Altogether, these results demonstrated that SlExp1 loss of function mutants yield firmer and late ripening fruits through modification of hemicellulose structure.
26566837	9	47	theme	wall	1384:1387	arg1	dynamics					1413:1420	the cell wall polysaccharide assembly dynamics	1375:1420	the cell wall polysaccharide assembly dynamics in fleshy fruits	1375:1437	These SlExp1 mutants represent good tools for breeding long shelf life tomato lines with contrasted fruit texture as well as for the understanding of the cell wall polysaccharide assembly dynamics in fleshy fruits.
26566837	9	48	theme	dynamics	1413:1420	arg1	understanding					1358:1370	the understanding	1354:1370	the understanding of the cell wall polysaccharide assembly dynamics in fleshy fruits	1354:1437	These SlExp1 mutants represent good tools for breeding long shelf life tomato lines with contrasted fruit texture as well as for the understanding of the cell wall polysaccharide assembly dynamics in fleshy fruits.
26566837	6	49	theme	neutral	823:829	arg1	sugar					831:835	neutral sugar	823:835	neutral sugar	823:835	Analysis of cell wall polysaccharide composition of Slexp1-7_Q213Stop mutant pointed out significant differences for uronic acid, neutral sugar and total sugar contents.
26566837	6	50	theme	wall	710:713	arg1	composition					730:740	cell wall polysaccharide composition	705:740	cell wall polysaccharide composition of Slexp1-7_Q213Stop mutant	705:768	Analysis of cell wall polysaccharide composition of Slexp1-7_Q213Stop mutant pointed out significant differences for uronic acid, neutral sugar and total sugar contents.
26566837	9	51	theme	SlExp1	1231:1236	arg1	mutants					1238:1244	These SlExp1 mutants	1225:1244	These SlExp1 mutants	1225:1244	These SlExp1 mutants represent good tools for breeding long shelf life tomato lines with contrasted fruit texture as well as for the understanding of the cell wall polysaccharide assembly dynamics in fleshy fruits.
26566837	0	52	theme	wall	46:49	arg1	metabolism					51:60	cell wall metabolism	41:60	cell wall metabolism	41:60	Induced mutations in tomato SlExp1 alter cell wall metabolism and delay fruit softening.
26566837	9	53	theme	polysaccharide	1389:1402	arg1	dynamics					1413:1420	the cell wall polysaccharide assembly dynamics	1375:1420	the cell wall polysaccharide assembly dynamics in fleshy fruits	1375:1437	These SlExp1 mutants represent good tools for breeding long shelf life tomato lines with contrasted fruit texture as well as for the understanding of the cell wall polysaccharide assembly dynamics in fleshy fruits.
26566837	8	54	theme	mutants	1130:1136	arg1	loss					1113:1116	SlExp1 loss	1106:1116	SlExp1 loss of function mutants	1106:1136	Altogether, these results demonstrated that SlExp1 loss of function mutants yield firmer and late ripening fruits through modification of hemicellulose structure.
26566837	9	55	theme	shelf	1285:1289	arg1	lines					1303:1307	breeding long shelf life tomato lines	1271:1307	breeding long shelf life tomato lines with contrasted fruit texture as well as for the understanding of the cell wall polysaccharide assembly dynamics in fleshy fruits	1271:1437	These SlExp1 mutants represent good tools for breeding long shelf life tomato lines with contrasted fruit texture as well as for the understanding of the cell wall polysaccharide assembly dynamics in fleshy fruits.
26566837	6	56	theme	sugar	847:851	arg1	contents					853:860	total sugar contents	841:860	total sugar contents	841:860	Analysis of cell wall polysaccharide composition of Slexp1-7_Q213Stop mutant pointed out significant differences for uronic acid, neutral sugar and total sugar contents.
26566837	0	57	theme	cell	41:44	arg1	metabolism					51:60	cell wall metabolism	41:60	cell wall metabolism	41:60	Induced mutations in tomato SlExp1 alter cell wall metabolism and delay fruit softening.
26566837	9	58	theme	assembly	1404:1411	arg1	dynamics					1413:1420	the cell wall polysaccharide assembly dynamics	1375:1420	the cell wall polysaccharide assembly dynamics in fleshy fruits	1375:1437	These SlExp1 mutants represent good tools for breeding long shelf life tomato lines with contrasted fruit texture as well as for the understanding of the cell wall polysaccharide assembly dynamics in fleshy fruits.
26566837	5	59	theme	function	623:630	arg1	loss					615:618	SlExp1 loss	608:618	SlExp1 loss of function	608:630	Characterization of two induced mutations, Slexp1-6_W211S, and Slexp1-7_Q213Stop, showed that SlExp1 loss of function leads to enhanced fruit firmness and delayed fruit ripening.
26566837	2	60	theme	key	180:182	arg1	role					184:187	a key role	178:187	a key role	178:187	Since cell walls play a key role in the softening process, expansins have been investigated to control fruit over ripening and deterioration.
26566837	9	61	theme	tomato	1296:1301	arg1	lines					1303:1307	breeding long shelf life tomato lines	1271:1307	breeding long shelf life tomato lines with contrasted fruit texture as well as for the understanding of the cell wall polysaccharide assembly dynamics in fleshy fruits	1271:1437	These SlExp1 mutants represent good tools for breeding long shelf life tomato lines with contrasted fruit texture as well as for the understanding of the cell wall polysaccharide assembly dynamics in fleshy fruits.
26566837	9	62	theme	fruit	1325:1329	arg1	texture					1331:1337	contrasted fruit texture	1314:1337	contrasted fruit texture	1314:1337	These SlExp1 mutants represent good tools for breeding long shelf life tomato lines with contrasted fruit texture as well as for the understanding of the cell wall polysaccharide assembly dynamics in fleshy fruits.
26566837	9	63	theme	long	1280:1283	arg1	lines					1303:1307	breeding long shelf life tomato lines	1271:1307	breeding long shelf life tomato lines with contrasted fruit texture as well as for the understanding of the cell wall polysaccharide assembly dynamics in fleshy fruits	1271:1437	These SlExp1 mutants represent good tools for breeding long shelf life tomato lines with contrasted fruit texture as well as for the understanding of the cell wall polysaccharide assembly dynamics in fleshy fruits.
26566837	4	64	theme	mutant	471:476	arg1	plants					478:483	mutant plants	471:483	mutant plants impaired in SlExp1 function	471:511	To engineer tomato plants with long shelf life, we screened for mutant plants impaired in SlExp1 function.
26566837	7	65	theme	Slexp1-7_Q213Stop	1036:1052	arg1	mutant					1054:1059	Slexp1-7_Q213Stop mutant	1036:1059	Slexp1-7_Q213Stop mutant	1036:1059	Hemicelluloses chemistry analysis by endo-β-1,4-d-glucanase hydrolysis and MALDI-TOF spectrometry revealed that xyloglucan structures were affected in the fruit pericarp of Slexp1-7_Q213Stop mutant.
26566837	6	66	theme	uronic	810:815	arg1	acid					817:820	uronic acid	810:820	uronic acid	810:820	Analysis of cell wall polysaccharide composition of Slexp1-7_Q213Stop mutant pointed out significant differences for uronic acid, neutral sugar and total sugar contents.
26566837	9	67	theme	cell	1379:1382	arg1	dynamics					1413:1420	the cell wall polysaccharide assembly dynamics	1375:1420	the cell wall polysaccharide assembly dynamics in fleshy fruits	1375:1437	These SlExp1 mutants represent good tools for breeding long shelf life tomato lines with contrasted fruit texture as well as for the understanding of the cell wall polysaccharide assembly dynamics in fleshy fruits.
26566837	7	68	theme	xyloglucan	975:984	arg1	structures					986:995	xyloglucan structures	975:995	xyloglucan structures	975:995	Hemicelluloses chemistry analysis by endo-β-1,4-d-glucanase hydrolysis and MALDI-TOF spectrometry revealed that xyloglucan structures were affected in the fruit pericarp of Slexp1-7_Q213Stop mutant.
26566837	7	69	theme	mutant	1054:1059	arg1	pericarp					1024:1031	the fruit pericarp	1014:1031	the fruit pericarp of Slexp1-7_Q213Stop mutant	1014:1059	Hemicelluloses chemistry analysis by endo-β-1,4-d-glucanase hydrolysis and MALDI-TOF spectrometry revealed that xyloglucan structures were affected in the fruit pericarp of Slexp1-7_Q213Stop mutant.
26566837	1	70	theme	key	122:124	arg1	softening					108:116	softening	108:116	softening	108:116	Fruit ripening and softening are key traits for many fleshy fruit.
26566837	1	70	theme	key	122:124	arg1	traits					126:131	key traits	122:131	key traits for many fleshy fruit	122:153	Fruit ripening and softening are key traits for many fleshy fruit.
26566837	1	70	theme	key	122:124	arg1	ripening					95:102	Fruit ripening	89:102	Fruit ripening	89:102	Fruit ripening and softening are key traits for many fleshy fruit.
26566837	7	71	theme	Hemicelluloses	863:876	arg1	analysis					888:895	Hemicelluloses chemistry analysis	863:895	Hemicelluloses chemistry analysis by endo-β-1,4-d-glucanase hydrolysis and MALDI-TOF spectrometry	863:959	Hemicelluloses chemistry analysis by endo-β-1,4-d-glucanase hydrolysis and MALDI-TOF spectrometry revealed that xyloglucan structures were affected in the fruit pericarp of Slexp1-7_Q213Stop mutant.
26566837	9	72	theme	fleshy	1425:1430	arg1	fruits					1432:1437	fleshy fruits	1425:1437	fleshy fruits	1425:1437	These SlExp1 mutants represent good tools for breeding long shelf life tomato lines with contrasted fruit texture as well as for the understanding of the cell wall polysaccharide assembly dynamics in fleshy fruits.
26566837	8	73	theme	late	1155:1158	arg1	fruits					1169:1174	firmer and late ripening fruits	1144:1174	firmer and late ripening fruits	1144:1174	Altogether, these results demonstrated that SlExp1 loss of function mutants yield firmer and late ripening fruits through modification of hemicellulose structure.
26566837	7	74	theme	fruit	1018:1022	arg1	pericarp					1024:1031	the fruit pericarp	1014:1031	the fruit pericarp of Slexp1-7_Q213Stop mutant	1014:1059	Hemicelluloses chemistry analysis by endo-β-1,4-d-glucanase hydrolysis and MALDI-TOF spectrometry revealed that xyloglucan structures were affected in the fruit pericarp of Slexp1-7_Q213Stop mutant.
26566837	7	75	theme	chemistry	878:886	arg1	analysis					888:895	Hemicelluloses chemistry analysis	863:895	Hemicelluloses chemistry analysis by endo-β-1,4-d-glucanase hydrolysis and MALDI-TOF spectrometry	863:959	Hemicelluloses chemistry analysis by endo-β-1,4-d-glucanase hydrolysis and MALDI-TOF spectrometry revealed that xyloglucan structures were affected in the fruit pericarp of Slexp1-7_Q213Stop mutant.
26566837	9	76	theme	contrasted	1314:1323	arg1	texture					1331:1337	contrasted fruit texture	1314:1337	contrasted fruit texture	1314:1337	These SlExp1 mutants represent good tools for breeding long shelf life tomato lines with contrasted fruit texture as well as for the understanding of the cell wall polysaccharide assembly dynamics in fleshy fruits.
26566837	5	77	theme	fruit	677:681	arg1	ripening					683:690	delayed fruit ripening	669:690	delayed fruit ripening	669:690	Characterization of two induced mutations, Slexp1-6_W211S, and Slexp1-7_Q213Stop, showed that SlExp1 loss of function leads to enhanced fruit firmness and delayed fruit ripening.
25624239	3	0	theme	-β-d-fructofuranosyl	654:673	arg1	1-kestose					639:647	1-kestose	639:647	1-kestose	639:647	Beside glucose and fructose and lower sucrose content, three short chain fructooligosaccharides were identified in ackee fruit, namely 1-kestose (1(F)-β-d-fructofuranosyl sucrose), nystose (1(F)(1-β-d-fructofuranosyl)2 sucrose) and DP5 (1(F)(1-β-d-fructofuranosyl)3 sucrose), while in carambola and June plum DP5 (1(F)(1-β-d-fructofuranosyl)3 sucrose) was not detected.
25624239	3	0	theme	-β-d-fructofuranosyl	654:673	arg1	sucrose					675:681	1(F)-β-d-fructofuranosyl sucrose	650:681	1(F)-β-d-fructofuranosyl sucrose	650:681	Beside glucose and fructose and lower sucrose content, three short chain fructooligosaccharides were identified in ackee fruit, namely 1-kestose (1(F)-β-d-fructofuranosyl sucrose), nystose (1(F)(1-β-d-fructofuranosyl)2 sucrose) and DP5 (1(F)(1-β-d-fructofuranosyl)3 sucrose), while in carambola and June plum DP5 (1(F)(1-β-d-fructofuranosyl)3 sucrose) was not detected.
25624239	1	1	theme	quality	262:268	arg1	attributes					270:279	their quality attributes	256:279	their quality attributes	256:279	The maturation of fruits is characterized by numerous compositional changes during ripening and these changes contribute in their quality attributes.
25624239	2	2	dep	June	449:452	arg1	plum					454:457	June plum (Spondias dulcis)	449:475	June plum (Spondias dulcis)	449:475	This study aimed to assess the contents of saccharides and potential fructooligosaccharides (FOS) of ackee (Blighia sapida Köenig), carambola (Averrhoa carambola) and June plum (Spondias dulcis), at green and ripe stages.
25624239	2	3	dep	Spondias	460:467	arg1	dulcis					469:474	Spondias dulcis	460:474	Spondias dulcis	460:474	This study aimed to assess the contents of saccharides and potential fructooligosaccharides (FOS) of ackee (Blighia sapida Köenig), carambola (Averrhoa carambola) and June plum (Spondias dulcis), at green and ripe stages.
25624239	0	4	theme	sapida	97:102	arg1	composition					39:49	Saccharides and fructooligosaccharides composition	0:49	Saccharides and fructooligosaccharides composition of green and ripe Averrhoa carambola, Blighia sapida and Spondias dulcis fruits.	0:130	Saccharides and fructooligosaccharides composition of green and ripe Averrhoa carambola, Blighia sapida and Spondias dulcis fruits.
25624239	0	5	theme	dulcis	117:122	arg1	fruits					124:129	dulcis fruits	117:129	dulcis fruits	117:129	Saccharides and fructooligosaccharides composition of green and ripe Averrhoa carambola, Blighia sapida and Spondias dulcis fruits.
25624239	0	6	theme	Spondias	108:115	arg1	composition					39:49	Saccharides and fructooligosaccharides composition	0:49	Saccharides and fructooligosaccharides composition of green and ripe Averrhoa carambola, Blighia sapida and Spondias dulcis fruits.	0:130	Saccharides and fructooligosaccharides composition of green and ripe Averrhoa carambola, Blighia sapida and Spondias dulcis fruits.
25624239	0	7	dep	sapida	97:102	arg1	fruits					124:129	dulcis fruits	117:129	dulcis fruits	117:129	Saccharides and fructooligosaccharides composition of green and ripe Averrhoa carambola, Blighia sapida and Spondias dulcis fruits.
25624239	1	8	theme	numerous	177:184	arg1	changes					200:206	numerous compositional changes	177:206	numerous compositional changes during ripening	177:222	The maturation of fruits is characterized by numerous compositional changes during ripening and these changes contribute in their quality attributes.
25624239	2	9	theme	saccharides	325:335	arg1	contents					313:320	the contents	309:320	the contents of saccharides and potential fructooligosaccharides (FOS) of ackee (Blighia sapida Köenig), carambola (Averrhoa carambola) and June plum (Spondias dulcis)	309:475	This study aimed to assess the contents of saccharides and potential fructooligosaccharides (FOS) of ackee (Blighia sapida Köenig), carambola (Averrhoa carambola) and June plum (Spondias dulcis), at green and ripe stages.
25624239	1	10	theme	compositional	186:198	arg1	changes					200:206	numerous compositional changes	177:206	numerous compositional changes during ripening	177:222	The maturation of fruits is characterized by numerous compositional changes during ripening and these changes contribute in their quality attributes.
25624239	0	11	theme	Saccharides	0:10	arg1	composition					39:49	Saccharides and fructooligosaccharides composition	0:49	Saccharides and fructooligosaccharides composition of green and ripe Averrhoa carambola, Blighia sapida and Spondias dulcis fruits.	0:130	Saccharides and fructooligosaccharides composition of green and ripe Averrhoa carambola, Blighia sapida and Spondias dulcis fruits.
25624239	3	12	theme	sucrose	542:548	arg1	fructooligosaccharides					577:598	three short chain fructooligosaccharides	559:598	three short chain fructooligosaccharides	559:598	Beside glucose and fructose and lower sucrose content, three short chain fructooligosaccharides were identified in ackee fruit, namely 1-kestose (1(F)-β-d-fructofuranosyl sucrose), nystose (1(F)(1-β-d-fructofuranosyl)2 sucrose) and DP5 (1(F)(1-β-d-fructofuranosyl)3 sucrose), while in carambola and June plum DP5 (1(F)(1-β-d-fructofuranosyl)3 sucrose) was not detected.
25624239	3	12	theme	sucrose	542:548	arg1	content					550:556	lower sucrose content	536:556	lower sucrose content	536:556	Beside glucose and fructose and lower sucrose content, three short chain fructooligosaccharides were identified in ackee fruit, namely 1-kestose (1(F)-β-d-fructofuranosyl sucrose), nystose (1(F)(1-β-d-fructofuranosyl)2 sucrose) and DP5 (1(F)(1-β-d-fructofuranosyl)3 sucrose), while in carambola and June plum DP5 (1(F)(1-β-d-fructofuranosyl)3 sucrose) was not detected.
25624239	3	13	theme	plum	808:811	arg1	DP5					813:815	June plum DP5	803:815	June plum DP5	803:815	Beside glucose and fructose and lower sucrose content, three short chain fructooligosaccharides were identified in ackee fruit, namely 1-kestose (1(F)-β-d-fructofuranosyl sucrose), nystose (1(F)(1-β-d-fructofuranosyl)2 sucrose) and DP5 (1(F)(1-β-d-fructofuranosyl)3 sucrose), while in carambola and June plum DP5 (1(F)(1-β-d-fructofuranosyl)3 sucrose) was not detected.
25624239	4	14	theme	Ripening	874:881	arg1	stage					883:887	Ripening stage	874:887	Ripening stage	874:887	Ripening stage also affected significantly the contents of these saccharides and sFOS.
25624239	3	15	theme	F	696:696	arg1	sucrose					723:729	1(F)(1-β-d-fructofuranosyl)2 sucrose	694:729	1(F)(1-β-d-fructofuranosyl)2 sucrose	694:729	Beside glucose and fructose and lower sucrose content, three short chain fructooligosaccharides were identified in ackee fruit, namely 1-kestose (1(F)-β-d-fructofuranosyl sucrose), nystose (1(F)(1-β-d-fructofuranosyl)2 sucrose) and DP5 (1(F)(1-β-d-fructofuranosyl)3 sucrose), while in carambola and June plum DP5 (1(F)(1-β-d-fructofuranosyl)3 sucrose) was not detected.
25624239	3	15	theme	F	696:696	arg1	nystose					685:691	nystose	685:691	nystose (1(F)(1-β-d-fructofuranosyl)2 sucrose)	685:730	Beside glucose and fructose and lower sucrose content, three short chain fructooligosaccharides were identified in ackee fruit, namely 1-kestose (1(F)-β-d-fructofuranosyl sucrose), nystose (1(F)(1-β-d-fructofuranosyl)2 sucrose) and DP5 (1(F)(1-β-d-fructofuranosyl)3 sucrose), while in carambola and June plum DP5 (1(F)(1-β-d-fructofuranosyl)3 sucrose) was not detected.
25624239	4	16	theme	saccharides	939:949	arg1	contents					921:928	the contents	917:928	the contents of these saccharides and sFOS	917:958	Ripening stage also affected significantly the contents of these saccharides and sFOS.
25624239	0	17	theme	fructooligosaccharides	16:37	arg1	composition					39:49	Saccharides and fructooligosaccharides composition	0:49	Saccharides and fructooligosaccharides composition of green and ripe Averrhoa carambola, Blighia sapida and Spondias dulcis fruits.	0:130	Saccharides and fructooligosaccharides composition of green and ripe Averrhoa carambola, Blighia sapida and Spondias dulcis fruits.
25624239	4	18	theme	sFOS	955:958	arg1	contents					921:928	the contents	917:928	the contents of these saccharides and sFOS	917:958	Ripening stage also affected significantly the contents of these saccharides and sFOS.
25624239	3	19	theme	June	803:806	arg1	DP5					813:815	June plum DP5	803:815	June plum DP5	803:815	Beside glucose and fructose and lower sucrose content, three short chain fructooligosaccharides were identified in ackee fruit, namely 1-kestose (1(F)-β-d-fructofuranosyl sucrose), nystose (1(F)(1-β-d-fructofuranosyl)2 sucrose) and DP5 (1(F)(1-β-d-fructofuranosyl)3 sucrose), while in carambola and June plum DP5 (1(F)(1-β-d-fructofuranosyl)3 sucrose) was not detected.
25624239	3	20	theme	1-β-d-fructofuranosyl	699:719	arg1	sucrose					723:729	1(F)(1-β-d-fructofuranosyl)2 sucrose	694:729	1(F)(1-β-d-fructofuranosyl)2 sucrose	694:729	Beside glucose and fructose and lower sucrose content, three short chain fructooligosaccharides were identified in ackee fruit, namely 1-kestose (1(F)-β-d-fructofuranosyl sucrose), nystose (1(F)(1-β-d-fructofuranosyl)2 sucrose) and DP5 (1(F)(1-β-d-fructofuranosyl)3 sucrose), while in carambola and June plum DP5 (1(F)(1-β-d-fructofuranosyl)3 sucrose) was not detected.
25624239	3	20	theme	1-β-d-fructofuranosyl	699:719	arg1	nystose					685:691	nystose	685:691	nystose (1(F)(1-β-d-fructofuranosyl)2 sucrose)	685:730	Beside glucose and fructose and lower sucrose content, three short chain fructooligosaccharides were identified in ackee fruit, namely 1-kestose (1(F)-β-d-fructofuranosyl sucrose), nystose (1(F)(1-β-d-fructofuranosyl)2 sucrose) and DP5 (1(F)(1-β-d-fructofuranosyl)3 sucrose), while in carambola and June plum DP5 (1(F)(1-β-d-fructofuranosyl)3 sucrose) was not detected.
25624239	3	21	theme	short	565:569	arg1	fructooligosaccharides					577:598	three short chain fructooligosaccharides	559:598	three short chain fructooligosaccharides	559:598	Beside glucose and fructose and lower sucrose content, three short chain fructooligosaccharides were identified in ackee fruit, namely 1-kestose (1(F)-β-d-fructofuranosyl sucrose), nystose (1(F)(1-β-d-fructofuranosyl)2 sucrose) and DP5 (1(F)(1-β-d-fructofuranosyl)3 sucrose), while in carambola and June plum DP5 (1(F)(1-β-d-fructofuranosyl)3 sucrose) was not detected.
25624239	3	21	theme	short	565:569	arg1	content					550:556	lower sucrose content	536:556	lower sucrose content	536:556	Beside glucose and fructose and lower sucrose content, three short chain fructooligosaccharides were identified in ackee fruit, namely 1-kestose (1(F)-β-d-fructofuranosyl sucrose), nystose (1(F)(1-β-d-fructofuranosyl)2 sucrose) and DP5 (1(F)(1-β-d-fructofuranosyl)3 sucrose), while in carambola and June plum DP5 (1(F)(1-β-d-fructofuranosyl)3 sucrose) was not detected.
25624239	2	22	theme	green	481:485	arg1	stages					496:501	green and ripe stages	481:501	green and ripe stages	481:501	This study aimed to assess the contents of saccharides and potential fructooligosaccharides (FOS) of ackee (Blighia sapida Köenig), carambola (Averrhoa carambola) and June plum (Spondias dulcis), at green and ripe stages.
25624239	3	23	theme	ackee	619:623	arg1	fruit					625:629	ackee fruit	619:629	ackee fruit	619:629	Beside glucose and fructose and lower sucrose content, three short chain fructooligosaccharides were identified in ackee fruit, namely 1-kestose (1(F)-β-d-fructofuranosyl sucrose), nystose (1(F)(1-β-d-fructofuranosyl)2 sucrose) and DP5 (1(F)(1-β-d-fructofuranosyl)3 sucrose), while in carambola and June plum DP5 (1(F)(1-β-d-fructofuranosyl)3 sucrose) was not detected.
25624239	3	24	theme	F	820:820	arg1	carambola					789:797	carambola	789:797	carambola	789:797	Beside glucose and fructose and lower sucrose content, three short chain fructooligosaccharides were identified in ackee fruit, namely 1-kestose (1(F)-β-d-fructofuranosyl sucrose), nystose (1(F)(1-β-d-fructofuranosyl)2 sucrose) and DP5 (1(F)(1-β-d-fructofuranosyl)3 sucrose), while in carambola and June plum DP5 (1(F)(1-β-d-fructofuranosyl)3 sucrose) was not detected.
25624239	3	24	theme	F	820:820	arg1	sucrose					847:853	1(F)(1-β-d-fructofuranosyl)3 sucrose	818:853	1(F)(1-β-d-fructofuranosyl)3 sucrose	818:853	Beside glucose and fructose and lower sucrose content, three short chain fructooligosaccharides were identified in ackee fruit, namely 1-kestose (1(F)-β-d-fructofuranosyl sucrose), nystose (1(F)(1-β-d-fructofuranosyl)2 sucrose) and DP5 (1(F)(1-β-d-fructofuranosyl)3 sucrose), while in carambola and June plum DP5 (1(F)(1-β-d-fructofuranosyl)3 sucrose) was not detected.
25624239	2	25	theme	June	449:452	arg1	contents					313:320	the contents	309:320	the contents of saccharides and potential fructooligosaccharides (FOS) of ackee (Blighia sapida Köenig), carambola (Averrhoa carambola) and June plum (Spondias dulcis)	309:475	This study aimed to assess the contents of saccharides and potential fructooligosaccharides (FOS) of ackee (Blighia sapida Köenig), carambola (Averrhoa carambola) and June plum (Spondias dulcis), at green and ripe stages.
25624239	2	26	theme	fructooligosaccharides	351:372	arg1	contents					313:320	the contents	309:320	the contents of saccharides and potential fructooligosaccharides (FOS) of ackee (Blighia sapida Köenig), carambola (Averrhoa carambola) and June plum (Spondias dulcis)	309:475	This study aimed to assess the contents of saccharides and potential fructooligosaccharides (FOS) of ackee (Blighia sapida Köenig), carambola (Averrhoa carambola) and June plum (Spondias dulcis), at green and ripe stages.
25624239	3	27	theme	lower	536:540	arg1	fructooligosaccharides					577:598	three short chain fructooligosaccharides	559:598	three short chain fructooligosaccharides	559:598	Beside glucose and fructose and lower sucrose content, three short chain fructooligosaccharides were identified in ackee fruit, namely 1-kestose (1(F)-β-d-fructofuranosyl sucrose), nystose (1(F)(1-β-d-fructofuranosyl)2 sucrose) and DP5 (1(F)(1-β-d-fructofuranosyl)3 sucrose), while in carambola and June plum DP5 (1(F)(1-β-d-fructofuranosyl)3 sucrose) was not detected.
25624239	3	27	theme	lower	536:540	arg1	content					550:556	lower sucrose content	536:556	lower sucrose content	536:556	Beside glucose and fructose and lower sucrose content, three short chain fructooligosaccharides were identified in ackee fruit, namely 1-kestose (1(F)-β-d-fructofuranosyl sucrose), nystose (1(F)(1-β-d-fructofuranosyl)2 sucrose) and DP5 (1(F)(1-β-d-fructofuranosyl)3 sucrose), while in carambola and June plum DP5 (1(F)(1-β-d-fructofuranosyl)3 sucrose) was not detected.
25624239	4	28	dep	saccharides	939:949	arg1	these					933:937	these	933:937	these	933:937	Ripening stage also affected significantly the contents of these saccharides and sFOS.
25624239	2	29	theme	potential	341:349	arg1	fructooligosaccharides					351:372	potential fructooligosaccharides	341:372	potential fructooligosaccharides (FOS)	341:378	This study aimed to assess the contents of saccharides and potential fructooligosaccharides (FOS) of ackee (Blighia sapida Köenig), carambola (Averrhoa carambola) and June plum (Spondias dulcis), at green and ripe stages.
25624239	2	29	theme	potential	341:349	arg1	FOS					375:377	FOS	375:377	FOS	375:377	This study aimed to assess the contents of saccharides and potential fructooligosaccharides (FOS) of ackee (Blighia sapida Köenig), carambola (Averrhoa carambola) and June plum (Spondias dulcis), at green and ripe stages.
25624239	3	30	theme	1-β-d-fructofuranosyl	746:766	arg1	sucrose					770:776	1(F)(1-β-d-fructofuranosyl)3 sucrose	741:776	1(F)(1-β-d-fructofuranosyl)3 sucrose	741:776	Beside glucose and fructose and lower sucrose content, three short chain fructooligosaccharides were identified in ackee fruit, namely 1-kestose (1(F)-β-d-fructofuranosyl sucrose), nystose (1(F)(1-β-d-fructofuranosyl)2 sucrose) and DP5 (1(F)(1-β-d-fructofuranosyl)3 sucrose), while in carambola and June plum DP5 (1(F)(1-β-d-fructofuranosyl)3 sucrose) was not detected.
25624239	3	30	theme	1-β-d-fructofuranosyl	746:766	arg1	DP5					736:738	DP5	736:738	DP5 (1(F)(1-β-d-fructofuranosyl)3 sucrose)	736:777	Beside glucose and fructose and lower sucrose content, three short chain fructooligosaccharides were identified in ackee fruit, namely 1-kestose (1(F)-β-d-fructofuranosyl sucrose), nystose (1(F)(1-β-d-fructofuranosyl)2 sucrose) and DP5 (1(F)(1-β-d-fructofuranosyl)3 sucrose), while in carambola and June plum DP5 (1(F)(1-β-d-fructofuranosyl)3 sucrose) was not detected.
25624239	3	31	theme	Beside	504:509	arg1	glucose					511:517	Beside glucose	504:517	Beside glucose	504:517	Beside glucose and fructose and lower sucrose content, three short chain fructooligosaccharides were identified in ackee fruit, namely 1-kestose (1(F)-β-d-fructofuranosyl sucrose), nystose (1(F)(1-β-d-fructofuranosyl)2 sucrose) and DP5 (1(F)(1-β-d-fructofuranosyl)3 sucrose), while in carambola and June plum DP5 (1(F)(1-β-d-fructofuranosyl)3 sucrose) was not detected.
25624239	3	32	theme	1-β-d-fructofuranosyl	823:843	arg1	carambola					789:797	carambola	789:797	carambola	789:797	Beside glucose and fructose and lower sucrose content, three short chain fructooligosaccharides were identified in ackee fruit, namely 1-kestose (1(F)-β-d-fructofuranosyl sucrose), nystose (1(F)(1-β-d-fructofuranosyl)2 sucrose) and DP5 (1(F)(1-β-d-fructofuranosyl)3 sucrose), while in carambola and June plum DP5 (1(F)(1-β-d-fructofuranosyl)3 sucrose) was not detected.
25624239	3	32	theme	1-β-d-fructofuranosyl	823:843	arg1	sucrose					847:853	1(F)(1-β-d-fructofuranosyl)3 sucrose	818:853	1(F)(1-β-d-fructofuranosyl)3 sucrose	818:853	Beside glucose and fructose and lower sucrose content, three short chain fructooligosaccharides were identified in ackee fruit, namely 1-kestose (1(F)-β-d-fructofuranosyl sucrose), nystose (1(F)(1-β-d-fructofuranosyl)2 sucrose) and DP5 (1(F)(1-β-d-fructofuranosyl)3 sucrose), while in carambola and June plum DP5 (1(F)(1-β-d-fructofuranosyl)3 sucrose) was not detected.
25624239	3	33	theme	F	743:743	arg1	sucrose					770:776	1(F)(1-β-d-fructofuranosyl)3 sucrose	741:776	1(F)(1-β-d-fructofuranosyl)3 sucrose	741:776	Beside glucose and fructose and lower sucrose content, three short chain fructooligosaccharides were identified in ackee fruit, namely 1-kestose (1(F)-β-d-fructofuranosyl sucrose), nystose (1(F)(1-β-d-fructofuranosyl)2 sucrose) and DP5 (1(F)(1-β-d-fructofuranosyl)3 sucrose), while in carambola and June plum DP5 (1(F)(1-β-d-fructofuranosyl)3 sucrose) was not detected.
25624239	3	33	theme	F	743:743	arg1	DP5					736:738	DP5	736:738	DP5 (1(F)(1-β-d-fructofuranosyl)3 sucrose)	736:777	Beside glucose and fructose and lower sucrose content, three short chain fructooligosaccharides were identified in ackee fruit, namely 1-kestose (1(F)-β-d-fructofuranosyl sucrose), nystose (1(F)(1-β-d-fructofuranosyl)2 sucrose) and DP5 (1(F)(1-β-d-fructofuranosyl)3 sucrose), while in carambola and June plum DP5 (1(F)(1-β-d-fructofuranosyl)3 sucrose) was not detected.
25624239	3	34	theme	chain	571:575	arg1	fructooligosaccharides					577:598	three short chain fructooligosaccharides	559:598	three short chain fructooligosaccharides	559:598	Beside glucose and fructose and lower sucrose content, three short chain fructooligosaccharides were identified in ackee fruit, namely 1-kestose (1(F)-β-d-fructofuranosyl sucrose), nystose (1(F)(1-β-d-fructofuranosyl)2 sucrose) and DP5 (1(F)(1-β-d-fructofuranosyl)3 sucrose), while in carambola and June plum DP5 (1(F)(1-β-d-fructofuranosyl)3 sucrose) was not detected.
25624239	3	34	theme	chain	571:575	arg1	content					550:556	lower sucrose content	536:556	lower sucrose content	536:556	Beside glucose and fructose and lower sucrose content, three short chain fructooligosaccharides were identified in ackee fruit, namely 1-kestose (1(F)-β-d-fructofuranosyl sucrose), nystose (1(F)(1-β-d-fructofuranosyl)2 sucrose) and DP5 (1(F)(1-β-d-fructofuranosyl)3 sucrose), while in carambola and June plum DP5 (1(F)(1-β-d-fructofuranosyl)3 sucrose) was not detected.
25624239	2	35	theme	ackee	383:387	arg1	contents					313:320	the contents	309:320	the contents of saccharides and potential fructooligosaccharides (FOS) of ackee (Blighia sapida Köenig), carambola (Averrhoa carambola) and June plum (Spondias dulcis)	309:475	This study aimed to assess the contents of saccharides and potential fructooligosaccharides (FOS) of ackee (Blighia sapida Köenig), carambola (Averrhoa carambola) and June plum (Spondias dulcis), at green and ripe stages.
25624239	1	36	theme	fruits	150:155	arg1	maturation					136:145	The maturation	132:145	The maturation of fruits	132:155	The maturation of fruits is characterized by numerous compositional changes during ripening and these changes contribute in their quality attributes.
25624239	2	37	theme	ripe	491:494	arg1	stages					496:501	green and ripe stages	481:501	green and ripe stages	481:501	This study aimed to assess the contents of saccharides and potential fructooligosaccharides (FOS) of ackee (Blighia sapida Köenig), carambola (Averrhoa carambola) and June plum (Spondias dulcis), at green and ripe stages.
24657376	5	0	theme	scanning	657:664	arg1	FESEM					687:691	FESEM	687:691	FESEM	687:691	The morphology of the nanocomposite prepared was analyzed by field emission scanning electron microscopy (FESEM) and transmission electron microscopy (TEM).
24657376	5	0	theme	scanning	657:664	arg1	microscopy					675:684	field emission scanning electron microscopy	642:684	field emission scanning electron microscopy (FESEM)	642:692	The morphology of the nanocomposite prepared was analyzed by field emission scanning electron microscopy (FESEM) and transmission electron microscopy (TEM).
24657376	1	1	theme	reinforcing	167:177	arg1	nanocrystals					118:129	Potato starch nanocrystals	104:129	Potato starch nanocrystals	104:129	Potato starch nanocrystals were found to serve as an effective reinforcing agent for natural rubber (NR).
24657376	1	1	theme	reinforcing	167:177	arg1	agent					179:183	an effective reinforcing agent	154:183	an effective reinforcing agent for natural rubber (NR)	154:207	Potato starch nanocrystals were found to serve as an effective reinforcing agent for natural rubber (NR).
24657376	11	2	theme	polymer	1424:1430	arg1	chains					1432:1437	the polymer chains	1420:1437	the polymer chains leading to an increase in the modulus and other mechanical properties	1420:1507	This network immobilizes the polymer chains leading to an increase in the modulus and other mechanical properties.
24657376	5	3	theme	electron	666:673	arg1	FESEM					687:691	FESEM	687:691	FESEM	687:691	The morphology of the nanocomposite prepared was analyzed by field emission scanning electron microscopy (FESEM) and transmission electron microscopy (TEM).
24657376	5	3	theme	electron	666:673	arg1	microscopy					675:684	field emission scanning electron microscopy	642:684	field emission scanning electron microscopy (FESEM)	642:692	The morphology of the nanocomposite prepared was analyzed by field emission scanning electron microscopy (FESEM) and transmission electron microscopy (TEM).
24657376	7	4	theme	filler	977:982	arg1	content					984:990	filler content	977:990	filler content	977:990	The crystallinity of the nanocomposites was studied using XRD analysis which indicated an overall increase in crystallinity with filler content.
24657376	7	5	theme	nanocomposites	873:886	arg1	crystallinity					852:864	The crystallinity	848:864	The crystallinity of the nanocomposites	848:886	The crystallinity of the nanocomposites was studied using XRD analysis which indicated an overall increase in crystallinity with filler content.
24657376	8	6	theme	stress-strain	1049:1061	arg1	behavior					1063:1070	stress-strain behavior	1049:1070	stress-strain behavior	1049:1070	The mechanical properties of the nanocomposites such as stress-strain behavior, tensile strength, tensile modulus and elongation at break were measured according to ASTM standards.
24657376	5	7	theme	nanocomposite	603:615	arg1	morphology					585:594	The morphology	581:594	The morphology of the nanocomposite prepared	581:624	The morphology of the nanocomposite prepared was analyzed by field emission scanning electron microscopy (FESEM) and transmission electron microscopy (TEM).
24657376	2	8	theme	granules	293:300	arg1	hydrolysis					265:274	the sulfuric acid hydrolysis	247:274	the sulfuric acid hydrolysis of potato starch granules	247:300	Starch nanocrystals were obtained by the sulfuric acid hydrolysis of potato starch granules.
24657376	3	9	theme	resulting	359:367	arg1	suspension					377:386	the resulting aqueous suspension	355:386	the resulting aqueous suspension	355:386	After mixing the latex and the starch nanocrystals, the resulting aqueous suspension was cast into film by solvent evaporation method.
24657376	2	10	theme	starch	286:291	arg1	granules					293:300	potato starch granules	279:300	potato starch granules	279:300	Starch nanocrystals were obtained by the sulfuric acid hydrolysis of potato starch granules.
24657376	8	11	from	break	1125:1129	arg1	behavior					1063:1070	stress-strain behavior	1049:1070	stress-strain behavior	1049:1070	The mechanical properties of the nanocomposites such as stress-strain behavior, tensile strength, tensile modulus and elongation at break were measured according to ASTM standards.
24657376	8	11	from	break	1125:1129	arg1	strength					1081:1088	tensile strength	1073:1088	tensile strength	1073:1088	The mechanical properties of the nanocomposites such as stress-strain behavior, tensile strength, tensile modulus and elongation at break were measured according to ASTM standards.
24657376	8	11	from	break	1125:1129	arg1	elongation					1111:1120	elongation	1111:1120	elongation at break	1111:1129	The mechanical properties of the nanocomposites such as stress-strain behavior, tensile strength, tensile modulus and elongation at break were measured according to ASTM standards.
24657376	8	11	from	break	1125:1129	arg1	modulus					1099:1105	tensile modulus	1091:1105	tensile modulus	1091:1105	The mechanical properties of the nanocomposites such as stress-strain behavior, tensile strength, tensile modulus and elongation at break were measured according to ASTM standards.
24657376	8	12	theme	mechanical	997:1006	arg1	properties					1008:1017	The mechanical properties	993:1017	The mechanical properties of the nanocomposites such as stress-strain behavior, tensile strength, tensile modulus and elongation at break	993:1129	The mechanical properties of the nanocomposites such as stress-strain behavior, tensile strength, tensile modulus and elongation at break were measured according to ASTM standards.
24657376	2	13	theme	potato	279:284	arg1	granules					293:300	potato starch granules	279:300	potato starch granules	279:300	Starch nanocrystals were obtained by the sulfuric acid hydrolysis of potato starch granules.
24657376	6	14	from	dispersion	818:827	arg1	composites					836:845	the composites	832:845	the composites	832:845	FESEM analysis revealed the size and shape of the crystal and their homogeneous dispersion in the composites.
24657376	4	15	theme	filler	498:503	arg1	loadings					505:512	filler loadings	498:512	filler loadings	498:512	The composite samples were successfully prepared by varying filler loadings, using a colloidal suspension of starch nanocrystals and NR latex.
24657376	8	16	theme	tensile	1073:1079	arg1	strength					1081:1088	tensile strength	1073:1088	tensile strength	1073:1088	The mechanical properties of the nanocomposites such as stress-strain behavior, tensile strength, tensile modulus and elongation at break were measured according to ASTM standards.
24657376	6	17	theme	crystal	788:794	arg1	shape					775:779	shape	775:779	shape	775:779	FESEM analysis revealed the size and shape of the crystal and their homogeneous dispersion in the composites.
24657376	6	17	theme	crystal	788:794	arg1	size					766:769	size	766:769	size	766:769	FESEM analysis revealed the size and shape of the crystal and their homogeneous dispersion in the composites.
24657376	6	17	theme	crystal	788:794	arg1	dispersion					818:827	their homogeneous dispersion	800:827	their homogeneous dispersion in the composites	800:845	FESEM analysis revealed the size and shape of the crystal and their homogeneous dispersion in the composites.
24657376	1	18	theme	natural	189:195	arg1	rubber					197:202	natural rubber	189:202	natural rubber (NR)	189:207	Potato starch nanocrystals were found to serve as an effective reinforcing agent for natural rubber (NR).
24657376	1	18	theme	natural	189:195	arg1	NR					205:206	NR	205:206	NR	205:206	Potato starch nanocrystals were found to serve as an effective reinforcing agent for natural rubber (NR).
24657376	5	19	theme	field	642:646	arg1	FESEM					687:691	FESEM	687:691	FESEM	687:691	The morphology of the nanocomposite prepared was analyzed by field emission scanning electron microscopy (FESEM) and transmission electron microscopy (TEM).
24657376	5	19	theme	field	642:646	arg1	microscopy					675:684	field emission scanning electron microscopy	642:684	field emission scanning electron microscopy (FESEM)	642:692	The morphology of the nanocomposite prepared was analyzed by field emission scanning electron microscopy (FESEM) and transmission electron microscopy (TEM).
24657376	8	20	theme	ASTM	1158:1161	arg1	standards					1163:1171	ASTM standards	1158:1171	ASTM standards	1158:1171	The mechanical properties of the nanocomposites such as stress-strain behavior, tensile strength, tensile modulus and elongation at break were measured according to ASTM standards.
24657376	1	21	theme	Potato	104:109	arg1	nanocrystals					118:129	Potato starch nanocrystals	104:129	Potato starch nanocrystals	104:129	Potato starch nanocrystals were found to serve as an effective reinforcing agent for natural rubber (NR).
24657376	1	21	theme	Potato	104:109	arg1	agent					179:183	an effective reinforcing agent	154:183	an effective reinforcing agent for natural rubber (NR)	154:207	Potato starch nanocrystals were found to serve as an effective reinforcing agent for natural rubber (NR).
24657376	3	22	theme	evaporation	418:428	arg1	method					430:435	solvent evaporation method	410:435	solvent evaporation method	410:435	After mixing the latex and the starch nanocrystals, the resulting aqueous suspension was cast into film by solvent evaporation method.
24657376	5	23	theme	emission	648:655	arg1	FESEM					687:691	FESEM	687:691	FESEM	687:691	The morphology of the nanocomposite prepared was analyzed by field emission scanning electron microscopy (FESEM) and transmission electron microscopy (TEM).
24657376	5	23	theme	emission	648:655	arg1	microscopy					675:684	field emission scanning electron microscopy	642:684	field emission scanning electron microscopy (FESEM)	642:692	The morphology of the nanocomposite prepared was analyzed by field emission scanning electron microscopy (FESEM) and transmission electron microscopy (TEM).
24657376	1	24	theme	starch	111:116	arg1	nanocrystals					118:129	Potato starch nanocrystals	104:129	Potato starch nanocrystals	104:129	Potato starch nanocrystals were found to serve as an effective reinforcing agent for natural rubber (NR).
24657376	1	24	theme	starch	111:116	arg1	agent					179:183	an effective reinforcing agent	154:183	an effective reinforcing agent for natural rubber (NR)	154:207	Potato starch nanocrystals were found to serve as an effective reinforcing agent for natural rubber (NR).
24657376	3	25	theme	aqueous	369:375	arg1	suspension					377:386	the resulting aqueous suspension	355:386	the resulting aqueous suspension	355:386	After mixing the latex and the starch nanocrystals, the resulting aqueous suspension was cast into film by solvent evaporation method.
24657376	4	26	theme	nanocrystals	554:565	arg1	suspension					533:542	a colloidal suspension	521:542	a colloidal suspension of starch nanocrystals and NR latex	521:578	The composite samples were successfully prepared by varying filler loadings, using a colloidal suspension of starch nanocrystals and NR latex.
24657376	3	27	dep	latex	320:324	arg1	nanocrystals					341:352	nanocrystals	341:352	nanocrystals	341:352	After mixing the latex and the starch nanocrystals, the resulting aqueous suspension was cast into film by solvent evaporation method.
24657376	7	28	theme	overall	938:944	arg1	increase					946:953	an overall increase	935:953	an overall increase in crystallinity with filler content	935:990	The crystallinity of the nanocomposites was studied using XRD analysis which indicated an overall increase in crystallinity with filler content.
24657376	9	29	theme	tensile	1178:1184	arg1	strength					1186:1193	tensile strength	1178:1193	tensile strength	1178:1193	The tensile strength and modulus of the composites were found to improve tremendously with increasing nanocrystal content.
24657376	4	30	theme	latex	574:578	arg1	suspension					533:542	a colloidal suspension	521:542	a colloidal suspension of starch nanocrystals and NR latex	521:578	The composite samples were successfully prepared by varying filler loadings, using a colloidal suspension of starch nanocrystals and NR latex.
24657376	11	31	theme	mechanical	1487:1496	arg1	properties					1498:1507	other mechanical properties	1481:1507	other mechanical properties	1481:1507	This network immobilizes the polymer chains leading to an increase in the modulus and other mechanical properties.
24657376	6	32	from	shape	775:779	arg1	composites					836:845	the composites	832:845	the composites	832:845	FESEM analysis revealed the size and shape of the crystal and their homogeneous dispersion in the composites.
24657376	0	33	theme	starch	43:48	arg1	nanocrystal					50:60	potato starch nanocrystal	36:60	potato starch nanocrystal	36:60	Preparation and characterization of potato starch nanocrystal reinforced natural rubber nanocomposites.
24657376	4	34	theme	NR	571:572	arg1	latex					574:578	NR latex	571:578	NR latex	571:578	The composite samples were successfully prepared by varying filler loadings, using a colloidal suspension of starch nanocrystals and NR latex.
24657376	4	35	theme	composite	442:450	arg1	samples					452:458	The composite samples	438:458	The composite samples	438:458	The composite samples were successfully prepared by varying filler loadings, using a colloidal suspension of starch nanocrystals and NR latex.
24657376	11	36	theme	other	1481:1485	arg1	properties					1498:1507	other mechanical properties	1481:1507	other mechanical properties	1481:1507	This network immobilizes the polymer chains leading to an increase in the modulus and other mechanical properties.
24657376	0	37	theme	potato	36:41	arg1	nanocrystal					50:60	potato starch nanocrystal	36:60	potato starch nanocrystal	36:60	Preparation and characterization of potato starch nanocrystal reinforced natural rubber nanocomposites.
24657376	7	38	with	crystallinity	958:970	arg1	content					984:990	filler content	977:990	filler content	977:990	The crystallinity of the nanocomposites was studied using XRD analysis which indicated an overall increase in crystallinity with filler content.
24657376	3	39	theme	solvent	410:416	arg1	method					430:435	solvent evaporation method	410:435	solvent evaporation method	410:435	After mixing the latex and the starch nanocrystals, the resulting aqueous suspension was cast into film by solvent evaporation method.
24657376	11	40	from	increase	1453:1460	arg1	properties					1498:1507	other mechanical properties	1481:1507	other mechanical properties	1481:1507	This network immobilizes the polymer chains leading to an increase in the modulus and other mechanical properties.
24657376	11	40	from	increase	1453:1460	arg1	modulus					1469:1475	the modulus	1465:1475	the modulus	1465:1475	This network immobilizes the polymer chains leading to an increase in the modulus and other mechanical properties.
24657376	10	41	theme	network	1386:1392	arg1	formation					1354:1362	the formation	1350:1362	the formation of starch nanocrystal network	1350:1392	This dramatic increase observed can be attributed to the formation of starch nanocrystal network.
24657376	9	42	theme	composites	1214:1223	arg1	modulus					1199:1205	modulus	1199:1205	modulus	1199:1205	The tensile strength and modulus of the composites were found to improve tremendously with increasing nanocrystal content.
24657376	9	42	theme	composites	1214:1223	arg1	strength					1186:1193	tensile strength	1178:1193	tensile strength	1178:1193	The tensile strength and modulus of the composites were found to improve tremendously with increasing nanocrystal content.
24657376	4	43	theme	colloidal	523:531	arg1	suspension					533:542	a colloidal suspension	521:542	a colloidal suspension of starch nanocrystals and NR latex	521:578	The composite samples were successfully prepared by varying filler loadings, using a colloidal suspension of starch nanocrystals and NR latex.
24657376	9	44	theme	nanocrystal	1276:1286	arg1	content					1288:1294	nanocrystal content	1276:1294	nanocrystal content	1276:1294	The tensile strength and modulus of the composites were found to improve tremendously with increasing nanocrystal content.
24657376	10	45	theme	nanocrystal	1374:1384	arg1	network					1386:1392	starch nanocrystal network	1367:1392	starch nanocrystal network	1367:1392	This dramatic increase observed can be attributed to the formation of starch nanocrystal network.
24657376	0	46	theme	nanocrystal	50:60	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of potato starch nanocrystal reinforced natural rubber nanocomposites.
24657376	0	46	theme	nanocrystal	50:60	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of potato starch nanocrystal reinforced natural rubber nanocomposites.
24657376	4	47	theme	starch	547:552	arg1	nanocrystals					554:565	starch nanocrystals	547:565	starch nanocrystals	547:565	The composite samples were successfully prepared by varying filler loadings, using a colloidal suspension of starch nanocrystals and NR latex.
24657376	5	48	theme	electron	711:718	arg1	microscopy					720:729	transmission electron microscopy	698:729	transmission electron microscopy (TEM)	698:735	The morphology of the nanocomposite prepared was analyzed by field emission scanning electron microscopy (FESEM) and transmission electron microscopy (TEM).
24657376	5	48	theme	electron	711:718	arg1	TEM					732:734	TEM	732:734	TEM	732:734	The morphology of the nanocomposite prepared was analyzed by field emission scanning electron microscopy (FESEM) and transmission electron microscopy (TEM).
24657376	2	49	theme	Starch	210:215	arg1	nanocrystals					217:228	Starch nanocrystals	210:228	Starch nanocrystals	210:228	Starch nanocrystals were obtained by the sulfuric acid hydrolysis of potato starch granules.
24657376	10	50	theme	starch	1367:1372	arg1	network					1386:1392	starch nanocrystal network	1367:1392	starch nanocrystal network	1367:1392	This dramatic increase observed can be attributed to the formation of starch nanocrystal network.
24657376	8	51	theme	nanocomposites	1026:1039	arg1	properties					1008:1017	The mechanical properties	993:1017	The mechanical properties of the nanocomposites such as stress-strain behavior, tensile strength, tensile modulus and elongation at break	993:1129	The mechanical properties of the nanocomposites such as stress-strain behavior, tensile strength, tensile modulus and elongation at break were measured according to ASTM standards.
24657376	6	52	theme	homogeneous	806:816	arg1	dispersion					818:827	their homogeneous dispersion	800:827	their homogeneous dispersion in the composites	800:845	FESEM analysis revealed the size and shape of the crystal and their homogeneous dispersion in the composites.
24657376	7	53	from	increase	946:953	arg1	crystallinity					958:970	crystallinity	958:970	crystallinity with filler content	958:990	The crystallinity of the nanocomposites was studied using XRD analysis which indicated an overall increase in crystallinity with filler content.
24657376	5	54	theme	transmission	698:709	arg1	microscopy					720:729	transmission electron microscopy	698:729	transmission electron microscopy (TEM)	698:735	The morphology of the nanocomposite prepared was analyzed by field emission scanning electron microscopy (FESEM) and transmission electron microscopy (TEM).
24657376	5	54	theme	transmission	698:709	arg1	TEM					732:734	TEM	732:734	TEM	732:734	The morphology of the nanocomposite prepared was analyzed by field emission scanning electron microscopy (FESEM) and transmission electron microscopy (TEM).
24657376	2	55	theme	acid	260:263	arg1	hydrolysis					265:274	the sulfuric acid hydrolysis	247:274	the sulfuric acid hydrolysis of potato starch granules	247:300	Starch nanocrystals were obtained by the sulfuric acid hydrolysis of potato starch granules.
24657376	6	56	from	size	766:769	arg1	composites					836:845	the composites	832:845	the composites	832:845	FESEM analysis revealed the size and shape of the crystal and their homogeneous dispersion in the composites.
24657376	7	57	theme	XRD	906:908	arg1	analysis					910:917	XRD analysis	906:917	XRD analysis which indicated an overall increase in crystallinity with filler content	906:990	The crystallinity of the nanocomposites was studied using XRD analysis which indicated an overall increase in crystallinity with filler content.
24657376	0	58	theme	rubber	81:86	arg1	nanocomposites					88:101	natural rubber nanocomposites	73:101	natural rubber nanocomposites	73:101	Preparation and characterization of potato starch nanocrystal reinforced natural rubber nanocomposites.
24657376	2	59	theme	sulfuric	251:258	arg1	hydrolysis					265:274	the sulfuric acid hydrolysis	247:274	the sulfuric acid hydrolysis of potato starch granules	247:300	Starch nanocrystals were obtained by the sulfuric acid hydrolysis of potato starch granules.
24657376	6	60	theme	FESEM	738:742	arg1	analysis					744:751	FESEM analysis	738:751	FESEM analysis	738:751	FESEM analysis revealed the size and shape of the crystal and their homogeneous dispersion in the composites.
24657376	0	61	theme	natural	73:79	arg1	nanocomposites					88:101	natural rubber nanocomposites	73:101	natural rubber nanocomposites	73:101	Preparation and characterization of potato starch nanocrystal reinforced natural rubber nanocomposites.
24657376	8	62	theme	tensile	1091:1097	arg1	modulus					1099:1105	tensile modulus	1091:1105	tensile modulus	1091:1105	The mechanical properties of the nanocomposites such as stress-strain behavior, tensile strength, tensile modulus and elongation at break were measured according to ASTM standards.
24657376	1	63	theme	effective	157:165	arg1	nanocrystals					118:129	Potato starch nanocrystals	104:129	Potato starch nanocrystals	104:129	Potato starch nanocrystals were found to serve as an effective reinforcing agent for natural rubber (NR).
24657376	1	63	theme	effective	157:165	arg1	agent					179:183	an effective reinforcing agent	154:183	an effective reinforcing agent for natural rubber (NR)	154:207	Potato starch nanocrystals were found to serve as an effective reinforcing agent for natural rubber (NR).
24657376	10	64	theme	dramatic	1302:1309	arg1	increase					1311:1318	This dramatic increase	1297:1318	This dramatic increase observed	1297:1327	This dramatic increase observed can be attributed to the formation of starch nanocrystal network.
27393243	2	0	theme	mitochondrial	438:450	arg1	membrane					452:459	inner mitochondrial membrane	432:459	inner mitochondrial membrane (IMM)	432:465	Liposomes were prepared to simulate the lipid compositions of the inner leaflet of the plasma membrane, outer mitochondrial membrane (OMM), and inner mitochondrial membrane (IMM).
27393243	2	0	theme	mitochondrial	438:450	arg1	IMM					462:464	IMM	462:464	IMM	462:464	Liposomes were prepared to simulate the lipid compositions of the inner leaflet of the plasma membrane, outer mitochondrial membrane (OMM), and inner mitochondrial membrane (IMM).
27393243	7	1	from	freeze-thawed	1061:1073	arg1	solution					1088:1095	trehalose solution	1078:1095	trehalose solution	1078:1095	When mitochondria isolated from rat liver were freeze-thawed in trehalose solution, the OMM remained intact based on the absence of increased oxygen consumption when cytochrome c was added during oxidative phosphorylation (OXPHOS).
27393243	4	2	theme	control	786:792	arg1	%					816:816	29.3%	812:816	29.3% with AfrLEA2	812:829	Only OMM-like liposomes were significantly protected by AfrLEA2 and AfrLEA3m against freeze-thaw damage; at the highest protein:lipid mass ratio tested, leakage of CF was 56.3% of control with AfrLEA3m and 29.3% with AfrLEA2.
27393243	4	2	theme	control	786:792	arg1	control					786:792	control	786:792	control with AfrLEA3m	786:806	Only OMM-like liposomes were significantly protected by AfrLEA2 and AfrLEA3m against freeze-thaw damage; at the highest protein:lipid mass ratio tested, leakage of CF was 56.3% of control with AfrLEA3m and 29.3% with AfrLEA2.
27393243	4	2	theme	control	786:792	arg1	%					781:781	56.3%	777:781	56.3% of control with AfrLEA3m	777:806	Only OMM-like liposomes were significantly protected by AfrLEA2 and AfrLEA3m against freeze-thaw damage; at the highest protein:lipid mass ratio tested, leakage of CF was 56.3% of control with AfrLEA3m and 29.3% with AfrLEA2.
27393243	1	3	theme	proteins	141:148	arg1	capacity					95:102	The capacity	91:102	The capacity of Late Embryogenesis Abundant (LEA) proteins and trehalose to protect liposomes against freezing-induced damage	91:215	The capacity of Late Embryogenesis Abundant (LEA) proteins and trehalose to protect liposomes against freezing-induced damage was examined by measuring the leakage of 5(6)-carboxyfluorescein (CF).
27393243	2	4	theme	inner	432:436	arg1	membrane					452:459	inner mitochondrial membrane	432:459	inner mitochondrial membrane (IMM)	432:465	Liposomes were prepared to simulate the lipid compositions of the inner leaflet of the plasma membrane, outer mitochondrial membrane (OMM), and inner mitochondrial membrane (IMM).
27393243	2	4	theme	inner	432:436	arg1	IMM					462:464	IMM	462:464	IMM	462:464	Liposomes were prepared to simulate the lipid compositions of the inner leaflet of the plasma membrane, outer mitochondrial membrane (OMM), and inner mitochondrial membrane (IMM).
27393243	9	5	theme	pore	1574:1577	arg1	opening					1535:1541	transient opening	1525:1541	transient opening of the permeability transition pore, a procedure optimized for retention of OMM integrity	1525:1631	Trehalose then was loaded into the matrix (0.24 μmol/mg mitochondrial protein) by transient opening of the permeability transition pore, a procedure optimized for retention of OMM integrity.
27393243	6	6	theme	bilayer	1005:1011	arg1	sides					992:996	both sides	987:996	both sides of the bilayer	987:1011	The greatest stabilization during freezing occurred when trehalose was present on both sides of the bilayer.
27393243	6	7	theme	greatest	909:916	arg1	stabilization					918:930	The greatest stabilization	905:930	The greatest stabilization during freezing	905:946	The greatest stabilization during freezing occurred when trehalose was present on both sides of the bilayer.
27393243	10	8	theme	matrix	1733:1738	arg1	trehalose					1740:1748	external plus matrix trehalose	1719:1748	external plus matrix trehalose	1719:1748	Surprisingly, respiratory control ratios were not improved after freeze-thawing with external plus matrix trehalose, when compared to external trehalose alone.
27393243	9	9	theme	transition	1563:1572	arg1	pore					1574:1577	the permeability transition pore	1546:1577	the permeability transition pore	1546:1577	Trehalose then was loaded into the matrix (0.24 μmol/mg mitochondrial protein) by transient opening of the permeability transition pore, a procedure optimized for retention of OMM integrity.
27393243	9	9	theme	transition	1563:1572	arg1	procedure					1582:1590	a procedure	1580:1590	a procedure optimized for retention of OMM integrity	1580:1631	Trehalose then was loaded into the matrix (0.24 μmol/mg mitochondrial protein) by transient opening of the permeability transition pore, a procedure optimized for retention of OMM integrity.
27393243	7	10	attach	isolated	1032:1039	arg2	mitochondria					1019:1030	mitochondria	1019:1030	mitochondria isolated from rat liver	1019:1054	When mitochondria isolated from rat liver were freeze-thawed in trehalose solution, the OMM remained intact based on the absence of increased oxygen consumption when cytochrome c was added during oxidative phosphorylation (OXPHOS).
27393243	7	10	attach	isolated	1032:1039	arg1	liver					1050:1054	rat liver	1046:1054	rat liver	1046:1054	When mitochondria isolated from rat liver were freeze-thawed in trehalose solution, the OMM remained intact based on the absence of increased oxygen consumption when cytochrome c was added during oxidative phosphorylation (OXPHOS).
27393243	10	11	theme	external	1719:1726	arg1	trehalose					1740:1748	external plus matrix trehalose	1719:1748	external plus matrix trehalose	1719:1748	Surprisingly, respiratory control ratios were not improved after freeze-thawing with external plus matrix trehalose, when compared to external trehalose alone.
27393243	7	12	from	solution	1088:1095	arg1	freeze-thawed					1061:1073	freeze-thawed	1061:1073	freeze-thawed	1061:1073	When mitochondria isolated from rat liver were freeze-thawed in trehalose solution, the OMM remained intact based on the absence of increased oxygen consumption when cytochrome c was added during oxidative phosphorylation (OXPHOS).
27393243	7	13	theme	consumption	1163:1173	arg1	absence					1135:1141	the absence	1131:1141	the absence of increased oxygen consumption	1131:1173	When mitochondria isolated from rat liver were freeze-thawed in trehalose solution, the OMM remained intact based on the absence of increased oxygen consumption when cytochrome c was added during oxidative phosphorylation (OXPHOS).
27393243	2	14	theme	lipid	328:332	arg1	compositions					334:345	the lipid compositions	324:345	the lipid compositions of the inner leaflet of the plasma membrane, outer mitochondrial membrane (OMM), and inner mitochondrial membrane (IMM)	324:465	Liposomes were prepared to simulate the lipid compositions of the inner leaflet of the plasma membrane, outer mitochondrial membrane (OMM), and inner mitochondrial membrane (IMM).
27393243	8	15	theme	control	1258:1264	arg1	OXPHOS/LEAK					1274:1284	OXPHOS/LEAK	1274:1284	OXPHOS/LEAK	1274:1284	Respiratory control ratios (OXPHOS/LEAK) were depressed by only 30% after freeze-thawing in trehalose compared to non-frozen controls, which indicated some retention of OXPHOS capacity by the IMM.
27393243	8	15	theme	control	1258:1264	arg1	ratios					1266:1271	Respiratory control ratios	1246:1271	Respiratory control ratios (OXPHOS/LEAK)	1246:1285	Respiratory control ratios (OXPHOS/LEAK) were depressed by only 30% after freeze-thawing in trehalose compared to non-frozen controls, which indicated some retention of OXPHOS capacity by the IMM.
27393243	2	16	theme	membrane	382:389	arg1	leaflet					360:366	the inner leaflet	350:366	the inner leaflet of the plasma membrane, outer mitochondrial membrane (OMM), and inner mitochondrial membrane (IMM)	350:465	Liposomes were prepared to simulate the lipid compositions of the inner leaflet of the plasma membrane, outer mitochondrial membrane (OMM), and inner mitochondrial membrane (IMM).
27393243	3	17	theme	recombinant	472:482	arg1	proteins					488:495	Two recombinant LEA proteins	468:495	Two recombinant LEA proteins belonging to Group 3 (AfrLEA2 and AfrLEA3m)	468:539	Two recombinant LEA proteins belonging to Group 3 (AfrLEA2 and AfrLEA3m) were expressed and purified from embryos of Artemia franciscana.
27393243	4	18	theme	OMM-like	611:618	arg1	liposomes					620:628	Only OMM-like liposomes	606:628	Only OMM-like liposomes	606:628	Only OMM-like liposomes were significantly protected by AfrLEA2 and AfrLEA3m against freeze-thaw damage; at the highest protein:lipid mass ratio tested, leakage of CF was 56.3% of control with AfrLEA3m and 29.3% with AfrLEA2.
27393243	1	19	theme	trehalose	154:162	arg1	capacity					95:102	The capacity	91:102	The capacity of Late Embryogenesis Abundant (LEA) proteins and trehalose to protect liposomes against freezing-induced damage	91:215	The capacity of Late Embryogenesis Abundant (LEA) proteins and trehalose to protect liposomes against freezing-induced damage was examined by measuring the leakage of 5(6)-carboxyfluorescein (CF).
27393243	4	20	theme	CF	770:771	arg1	leakage					759:765	leakage	759:765	leakage of CF	759:771	Only OMM-like liposomes were significantly protected by AfrLEA2 and AfrLEA3m against freeze-thaw damage; at the highest protein:lipid mass ratio tested, leakage of CF was 56.3% of control with AfrLEA3m and 29.3% with AfrLEA2.
27393243	3	21	theme	LEA	484:486	arg1	proteins					488:495	Two recombinant LEA proteins	468:495	Two recombinant LEA proteins belonging to Group 3 (AfrLEA2 and AfrLEA3m)	468:539	Two recombinant LEA proteins belonging to Group 3 (AfrLEA2 and AfrLEA3m) were expressed and purified from embryos of Artemia franciscana.
27393243	2	22	theme	leaflet	360:366	arg1	compositions					334:345	the lipid compositions	324:345	the lipid compositions of the inner leaflet of the plasma membrane, outer mitochondrial membrane (OMM), and inner mitochondrial membrane (IMM)	324:465	Liposomes were prepared to simulate the lipid compositions of the inner leaflet of the plasma membrane, outer mitochondrial membrane (OMM), and inner mitochondrial membrane (IMM).
27393243	7	23	theme	oxidative	1210:1218	arg1	OXPHOS					1237:1242	OXPHOS	1237:1242	OXPHOS	1237:1242	When mitochondria isolated from rat liver were freeze-thawed in trehalose solution, the OMM remained intact based on the absence of increased oxygen consumption when cytochrome c was added during oxidative phosphorylation (OXPHOS).
27393243	7	23	theme	oxidative	1210:1218	arg1	phosphorylation					1220:1234	oxidative phosphorylation	1210:1234	oxidative phosphorylation (OXPHOS)	1210:1243	When mitochondria isolated from rat liver were freeze-thawed in trehalose solution, the OMM remained intact based on the absence of increased oxygen consumption when cytochrome c was added during oxidative phosphorylation (OXPHOS).
27393243	4	24	theme	freeze-thaw	691:701	arg1	damage					703:708	freeze-thaw damage	691:708	freeze-thaw damage	691:708	Only OMM-like liposomes were significantly protected by AfrLEA2 and AfrLEA3m against freeze-thaw damage; at the highest protein:lipid mass ratio tested, leakage of CF was 56.3% of control with AfrLEA3m and 29.3% with AfrLEA2.
27393243	2	25	theme	inner	354:358	arg1	leaflet					360:366	the inner leaflet	350:366	the inner leaflet of the plasma membrane, outer mitochondrial membrane (OMM), and inner mitochondrial membrane (IMM)	350:465	Liposomes were prepared to simulate the lipid compositions of the inner leaflet of the plasma membrane, outer mitochondrial membrane (OMM), and inner mitochondrial membrane (IMM).
27393243	2	26	theme	membrane	412:419	arg1	leaflet					360:366	the inner leaflet	350:366	the inner leaflet of the plasma membrane, outer mitochondrial membrane (OMM), and inner mitochondrial membrane (IMM)	350:465	Liposomes were prepared to simulate the lipid compositions of the inner leaflet of the plasma membrane, outer mitochondrial membrane (OMM), and inner mitochondrial membrane (IMM).
27393243	8	27	theme	non-frozen	1360:1369	arg1	controls					1371:1378	non-frozen controls	1360:1378	non-frozen controls	1360:1378	Respiratory control ratios (OXPHOS/LEAK) were depressed by only 30% after freeze-thawing in trehalose compared to non-frozen controls, which indicated some retention of OXPHOS capacity by the IMM.
27393243	0	28	theme	bilayers	26:33	arg1	Cryopreservation					0:15	Cryopreservation	0:15	Cryopreservation of lipid bilayers by LEA proteins from Artemia franciscana and trehalose	0:88	Cryopreservation of lipid bilayers by LEA proteins from Artemia franciscana and trehalose.
27393243	7	29	theme	rat	1046:1048	arg1	liver					1050:1054	rat liver	1046:1054	rat liver	1046:1054	When mitochondria isolated from rat liver were freeze-thawed in trehalose solution, the OMM remained intact based on the absence of increased oxygen consumption when cytochrome c was added during oxidative phosphorylation (OXPHOS).
27393243	8	30	theme	Respiratory	1246:1256	arg1	OXPHOS/LEAK					1274:1284	OXPHOS/LEAK	1274:1284	OXPHOS/LEAK	1274:1284	Respiratory control ratios (OXPHOS/LEAK) were depressed by only 30% after freeze-thawing in trehalose compared to non-frozen controls, which indicated some retention of OXPHOS capacity by the IMM.
27393243	8	30	theme	Respiratory	1246:1256	arg1	ratios					1266:1271	Respiratory control ratios	1246:1271	Respiratory control ratios (OXPHOS/LEAK)	1246:1285	Respiratory control ratios (OXPHOS/LEAK) were depressed by only 30% after freeze-thawing in trehalose compared to non-frozen controls, which indicated some retention of OXPHOS capacity by the IMM.
27393243	4	31	with	%	816:816	arg1	AfrLEA2					823:829	AfrLEA2	823:829	AfrLEA2	823:829	Only OMM-like liposomes were significantly protected by AfrLEA2 and AfrLEA3m against freeze-thaw damage; at the highest protein:lipid mass ratio tested, leakage of CF was 56.3% of control with AfrLEA3m and 29.3% with AfrLEA2.
27393243	11	32	theme	trehalose	1872:1880	arg1	accumulation					1849:1860	insufficient accumulation	1836:1860	insufficient accumulation of matrix trehalose	1836:1880	This result could perhaps be explained by insufficient accumulation of matrix trehalose.
27393243	2	33	theme	outer	392:396	arg1	membrane					412:419	outer mitochondrial membrane	392:419	outer mitochondrial membrane (OMM)	392:425	Liposomes were prepared to simulate the lipid compositions of the inner leaflet of the plasma membrane, outer mitochondrial membrane (OMM), and inner mitochondrial membrane (IMM).
27393243	2	33	theme	outer	392:396	arg1	OMM					422:424	OMM	422:424	OMM	422:424	Liposomes were prepared to simulate the lipid compositions of the inner leaflet of the plasma membrane, outer mitochondrial membrane (OMM), and inner mitochondrial membrane (IMM).
27393243	1	34	theme	-carboxyfluorescein	262:280	arg1	leakage					247:253	the leakage	243:253	the leakage of 5(6)-carboxyfluorescein (CF)	243:285	The capacity of Late Embryogenesis Abundant (LEA) proteins and trehalose to protect liposomes against freezing-induced damage was examined by measuring the leakage of 5(6)-carboxyfluorescein (CF).
27393243	4	35	theme	protein	726:732	arg1	ratio					745:749	the highest protein:lipid mass ratio	714:749	the highest protein:lipid mass ratio tested	714:756	Only OMM-like liposomes were significantly protected by AfrLEA2 and AfrLEA3m against freeze-thaw damage; at the highest protein:lipid mass ratio tested, leakage of CF was 56.3% of control with AfrLEA3m and 29.3% with AfrLEA2.
27393243	0	36	from	trehalose	80:88	arg1	proteins					42:49	LEA proteins	38:49	LEA proteins from Artemia franciscana and trehalose	38:88	Cryopreservation of lipid bilayers by LEA proteins from Artemia franciscana and trehalose.
27393243	7	37	theme	oxygen	1156:1161	arg1	consumption					1163:1173	increased oxygen consumption	1146:1173	increased oxygen consumption	1146:1173	When mitochondria isolated from rat liver were freeze-thawed in trehalose solution, the OMM remained intact based on the absence of increased oxygen consumption when cytochrome c was added during oxidative phosphorylation (OXPHOS).
27393243	1	38	theme	freezing-induced	193:208	arg1	damage					210:215	freezing-induced damage	193:215	freezing-induced damage	193:215	The capacity of Late Embryogenesis Abundant (LEA) proteins and trehalose to protect liposomes against freezing-induced damage was examined by measuring the leakage of 5(6)-carboxyfluorescein (CF).
27393243	0	39	theme	LEA	38:40	arg1	proteins					42:49	LEA proteins	38:49	LEA proteins from Artemia franciscana and trehalose	38:88	Cryopreservation of lipid bilayers by LEA proteins from Artemia franciscana and trehalose.
27393243	9	40	theme	integrity	1623:1631	arg1	retention					1606:1614	retention	1606:1614	retention of OMM integrity	1606:1631	Trehalose then was loaded into the matrix (0.24 μmol/mg mitochondrial protein) by transient opening of the permeability transition pore, a procedure optimized for retention of OMM integrity.
27393243	3	41	theme	Artemia	585:591	arg1	franciscana					593:603	Artemia franciscana	585:603	Artemia franciscana	585:603	Two recombinant LEA proteins belonging to Group 3 (AfrLEA2 and AfrLEA3m) were expressed and purified from embryos of Artemia franciscana.
27393243	2	42	theme	plasma	375:380	arg1	membrane					382:389	the plasma membrane	371:389	the plasma membrane	371:389	Liposomes were prepared to simulate the lipid compositions of the inner leaflet of the plasma membrane, outer mitochondrial membrane (OMM), and inner mitochondrial membrane (IMM).
27393243	7	43	theme	trehalose	1078:1086	arg1	solution					1088:1095	trehalose solution	1078:1095	trehalose solution	1078:1095	When mitochondria isolated from rat liver were freeze-thawed in trehalose solution, the OMM remained intact based on the absence of increased oxygen consumption when cytochrome c was added during oxidative phosphorylation (OXPHOS).
27393243	0	44	from	franciscana	64:74	arg1	proteins					42:49	LEA proteins	38:49	LEA proteins from Artemia franciscana and trehalose	38:88	Cryopreservation of lipid bilayers by LEA proteins from Artemia franciscana and trehalose.
27393243	2	45	theme	mitochondrial	398:410	arg1	membrane					412:419	outer mitochondrial membrane	392:419	outer mitochondrial membrane (OMM)	392:425	Liposomes were prepared to simulate the lipid compositions of the inner leaflet of the plasma membrane, outer mitochondrial membrane (OMM), and inner mitochondrial membrane (IMM).
27393243	2	45	theme	mitochondrial	398:410	arg1	OMM					422:424	OMM	422:424	OMM	422:424	Liposomes were prepared to simulate the lipid compositions of the inner leaflet of the plasma membrane, outer mitochondrial membrane (OMM), and inner mitochondrial membrane (IMM).
27393243	11	46	theme	matrix	1865:1870	arg1	trehalose					1872:1880	matrix trehalose	1865:1880	matrix trehalose	1865:1880	This result could perhaps be explained by insufficient accumulation of matrix trehalose.
27393243	0	47	theme	Artemia	56:62	arg1	franciscana					64:74	Artemia franciscana	56:74	Artemia franciscana	56:74	Cryopreservation of lipid bilayers by LEA proteins from Artemia franciscana and trehalose.
27393243	8	48	theme	OXPHOS	1415:1420	arg1	capacity					1422:1429	OXPHOS capacity	1415:1429	OXPHOS capacity	1415:1429	Respiratory control ratios (OXPHOS/LEAK) were depressed by only 30% after freeze-thawing in trehalose compared to non-frozen controls, which indicated some retention of OXPHOS capacity by the IMM.
27393243	8	49	theme	capacity	1422:1429	arg1	retention					1402:1410	some retention	1397:1410	some retention of OXPHOS capacity by the IMM	1397:1440	Respiratory control ratios (OXPHOS/LEAK) were depressed by only 30% after freeze-thawing in trehalose compared to non-frozen controls, which indicated some retention of OXPHOS capacity by the IMM.
27393243	1	50	theme	Late	107:110	arg1	Embryogenesis					112:124	Late Embryogenesis	107:124	Late Embryogenesis	107:124	The capacity of Late Embryogenesis Abundant (LEA) proteins and trehalose to protect liposomes against freezing-induced damage was examined by measuring the leakage of 5(6)-carboxyfluorescein (CF).
27393243	10	51	theme	respiratory	1648:1658	arg1	ratios					1668:1673	respiratory control ratios	1648:1673	respiratory control ratios	1648:1673	Surprisingly, respiratory control ratios were not improved after freeze-thawing with external plus matrix trehalose, when compared to external trehalose alone.
27393243	6	52	located	present	976:982	arg1	sides					992:996	both sides	987:996	both sides of the bilayer	987:1011	The greatest stabilization during freezing occurred when trehalose was present on both sides of the bilayer.
27393243	6	52	located	present	976:982	arg2	trehalose					962:970	trehalose	962:970	trehalose	962:970	The greatest stabilization during freezing occurred when trehalose was present on both sides of the bilayer.
27393243	9	53	theme	transient	1525:1533	arg1	opening					1535:1541	transient opening	1525:1541	transient opening of the permeability transition pore, a procedure optimized for retention of OMM integrity	1525:1631	Trehalose then was loaded into the matrix (0.24 μmol/mg mitochondrial protein) by transient opening of the permeability transition pore, a procedure optimized for retention of OMM integrity.
27393243	4	54	theme	mass	740:743	arg1	ratio					745:749	the highest protein:lipid mass ratio	714:749	the highest protein:lipid mass ratio tested	714:756	Only OMM-like liposomes were significantly protected by AfrLEA2 and AfrLEA3m against freeze-thaw damage; at the highest protein:lipid mass ratio tested, leakage of CF was 56.3% of control with AfrLEA3m and 29.3% with AfrLEA2.
27393243	10	55	theme	external	1768:1775	arg1	trehalose					1777:1785	external trehalose	1768:1785	external trehalose	1768:1785	Surprisingly, respiratory control ratios were not improved after freeze-thawing with external plus matrix trehalose, when compared to external trehalose alone.
27393243	4	56	theme	lipid	734:738	arg1	ratio					745:749	the highest protein:lipid mass ratio	714:749	the highest protein:lipid mass ratio tested	714:756	Only OMM-like liposomes were significantly protected by AfrLEA2 and AfrLEA3m against freeze-thaw damage; at the highest protein:lipid mass ratio tested, leakage of CF was 56.3% of control with AfrLEA3m and 29.3% with AfrLEA2.
27393243	9	57	theme	permeability	1550:1561	arg1	pore					1574:1577	the permeability transition pore	1546:1577	the permeability transition pore	1546:1577	Trehalose then was loaded into the matrix (0.24 μmol/mg mitochondrial protein) by transient opening of the permeability transition pore, a procedure optimized for retention of OMM integrity.
27393243	9	57	theme	permeability	1550:1561	arg1	procedure					1582:1590	a procedure	1580:1590	a procedure optimized for retention of OMM integrity	1580:1631	Trehalose then was loaded into the matrix (0.24 μmol/mg mitochondrial protein) by transient opening of the permeability transition pore, a procedure optimized for retention of OMM integrity.
27393243	1	58	theme	Abundant	126:133	arg1	proteins					141:148	Late Embryogenesis Abundant (LEA) proteins	107:148	Late Embryogenesis Abundant (LEA) proteins	107:148	The capacity of Late Embryogenesis Abundant (LEA) proteins and trehalose to protect liposomes against freezing-induced damage was examined by measuring the leakage of 5(6)-carboxyfluorescein (CF).
27393243	1	58	theme	Abundant	126:133	arg1	LEA					136:138	LEA	136:138	LEA	136:138	The capacity of Late Embryogenesis Abundant (LEA) proteins and trehalose to protect liposomes against freezing-induced damage was examined by measuring the leakage of 5(6)-carboxyfluorescein (CF).
27393243	9	59	theme	0.24 μmol/mg	1486:1497	arg1	protein					1513:1519	0.24 μmol/mg mitochondrial protein	1486:1519	0.24 μmol/mg mitochondrial protein	1486:1519	Trehalose then was loaded into the matrix (0.24 μmol/mg mitochondrial protein) by transient opening of the permeability transition pore, a procedure optimized for retention of OMM integrity.
27393243	9	59	theme	0.24 μmol/mg	1486:1497	arg1	matrix					1478:1483	the matrix	1474:1483	the matrix (0.24 μmol/mg mitochondrial protein)	1474:1520	Trehalose then was loaded into the matrix (0.24 μmol/mg mitochondrial protein) by transient opening of the permeability transition pore, a procedure optimized for retention of OMM integrity.
27393243	4	60	theme	highest	718:724	arg1	ratio					745:749	the highest protein:lipid mass ratio	714:749	the highest protein:lipid mass ratio tested	714:756	Only OMM-like liposomes were significantly protected by AfrLEA2 and AfrLEA3m against freeze-thaw damage; at the highest protein:lipid mass ratio tested, leakage of CF was 56.3% of control with AfrLEA3m and 29.3% with AfrLEA2.
27393243	3	61	theme	franciscana	593:603	arg1	embryos					574:580	embryos	574:580	embryos of Artemia franciscana	574:603	Two recombinant LEA proteins belonging to Group 3 (AfrLEA2 and AfrLEA3m) were expressed and purified from embryos of Artemia franciscana.
27393243	9	62	theme	OMM	1619:1621	arg1	integrity					1623:1631	OMM integrity	1619:1631	OMM integrity	1619:1631	Trehalose then was loaded into the matrix (0.24 μmol/mg mitochondrial protein) by transient opening of the permeability transition pore, a procedure optimized for retention of OMM integrity.
27393243	4	63	from	ratio	745:749	arg1	%					816:816	29.3%	812:816	29.3% with AfrLEA2	812:829	Only OMM-like liposomes were significantly protected by AfrLEA2 and AfrLEA3m against freeze-thaw damage; at the highest protein:lipid mass ratio tested, leakage of CF was 56.3% of control with AfrLEA3m and 29.3% with AfrLEA2.
27393243	4	63	from	ratio	745:749	arg1	control					786:792	control	786:792	control with AfrLEA3m	786:806	Only OMM-like liposomes were significantly protected by AfrLEA2 and AfrLEA3m against freeze-thaw damage; at the highest protein:lipid mass ratio tested, leakage of CF was 56.3% of control with AfrLEA3m and 29.3% with AfrLEA2.
27393243	4	63	from	ratio	745:749	arg1	%					781:781	56.3%	777:781	56.3% of control with AfrLEA3m	777:806	Only OMM-like liposomes were significantly protected by AfrLEA2 and AfrLEA3m against freeze-thaw damage; at the highest protein:lipid mass ratio tested, leakage of CF was 56.3% of control with AfrLEA3m and 29.3% with AfrLEA2.
27393243	7	64	theme	cytochrome	1180:1189	arg1	c					1191:1191	cytochrome c	1180:1191	cytochrome c	1180:1191	When mitochondria isolated from rat liver were freeze-thawed in trehalose solution, the OMM remained intact based on the absence of increased oxygen consumption when cytochrome c was added during oxidative phosphorylation (OXPHOS).
27393243	4	65	with	control	786:792	arg1	AfrLEA3m					799:806	AfrLEA3m	799:806	AfrLEA3m	799:806	Only OMM-like liposomes were significantly protected by AfrLEA2 and AfrLEA3m against freeze-thaw damage; at the highest protein:lipid mass ratio tested, leakage of CF was 56.3% of control with AfrLEA3m and 29.3% with AfrLEA2.
27393243	5	66	theme	compositional	884:896	arg1	types					898:902	all compositional types	880:902	all compositional types	880:902	By comparison, trehalose provided protection to all compositional types.
27393243	10	67	theme	control	1660:1666	arg1	ratios					1668:1673	respiratory control ratios	1648:1673	respiratory control ratios	1648:1673	Surprisingly, respiratory control ratios were not improved after freeze-thawing with external plus matrix trehalose, when compared to external trehalose alone.
27393243	7	68	theme	increased	1146:1154	arg1	consumption					1163:1173	increased oxygen consumption	1146:1173	increased oxygen consumption	1146:1173	When mitochondria isolated from rat liver were freeze-thawed in trehalose solution, the OMM remained intact based on the absence of increased oxygen consumption when cytochrome c was added during oxidative phosphorylation (OXPHOS).
27393243	9	69	theme	mitochondrial	1499:1511	arg1	protein					1513:1519	0.24 μmol/mg mitochondrial protein	1486:1519	0.24 μmol/mg mitochondrial protein	1486:1519	Trehalose then was loaded into the matrix (0.24 μmol/mg mitochondrial protein) by transient opening of the permeability transition pore, a procedure optimized for retention of OMM integrity.
27393243	9	69	theme	mitochondrial	1499:1511	arg1	matrix					1478:1483	the matrix	1474:1483	the matrix (0.24 μmol/mg mitochondrial protein)	1474:1520	Trehalose then was loaded into the matrix (0.24 μmol/mg mitochondrial protein) by transient opening of the permeability transition pore, a procedure optimized for retention of OMM integrity.
27393243	2	70	theme	membrane	452:459	arg1	leaflet					360:366	the inner leaflet	350:366	the inner leaflet of the plasma membrane, outer mitochondrial membrane (OMM), and inner mitochondrial membrane (IMM)	350:465	Liposomes were prepared to simulate the lipid compositions of the inner leaflet of the plasma membrane, outer mitochondrial membrane (OMM), and inner mitochondrial membrane (IMM).
27393243	6	71	attach	present	976:982	arg2	trehalose					962:970	trehalose	962:970	trehalose	962:970	The greatest stabilization during freezing occurred when trehalose was present on both sides of the bilayer.
27393243	6	71	attach	present	976:982	arg1	sides					992:996	both sides	987:996	both sides of the bilayer	987:1011	The greatest stabilization during freezing occurred when trehalose was present on both sides of the bilayer.
27393243	0	72	theme	lipid	20:24	arg1	bilayers					26:33	lipid bilayers	20:33	lipid bilayers	20:33	Cryopreservation of lipid bilayers by LEA proteins from Artemia franciscana and trehalose.
27393243	3	73	dep	Group	510:514	arg1	AfrLEA3m					531:538	AfrLEA3m	531:538	AfrLEA3m	531:538	Two recombinant LEA proteins belonging to Group 3 (AfrLEA2 and AfrLEA3m) were expressed and purified from embryos of Artemia franciscana.
27393243	3	73	dep	Group	510:514	arg1	AfrLEA2					519:525	AfrLEA2	519:525	AfrLEA2	519:525	Two recombinant LEA proteins belonging to Group 3 (AfrLEA2 and AfrLEA3m) were expressed and purified from embryos of Artemia franciscana.
27393243	4	74	with	%	781:781	arg1	AfrLEA2					823:829	AfrLEA2	823:829	AfrLEA2	823:829	Only OMM-like liposomes were significantly protected by AfrLEA2 and AfrLEA3m against freeze-thaw damage; at the highest protein:lipid mass ratio tested, leakage of CF was 56.3% of control with AfrLEA3m and 29.3% with AfrLEA2.
27393243	11	75	theme	insufficient	1836:1847	arg1	accumulation					1849:1860	insufficient accumulation	1836:1860	insufficient accumulation of matrix trehalose	1836:1880	This result could perhaps be explained by insufficient accumulation of matrix trehalose.
27281477	8	0	from	increase	1336:1343	arg1	OSP					1370:1372	endomembrane-situated OSP	1348:1372	endomembrane-situated OSP	1348:1372	The BFA-provoked increase in endomembrane-situated OSP is sensitive to nocodazole, and BFA causes partial redistribution of DLODP activity from GA- to ER-containing regions of density gradients.
27281477	1	1	theme	oligosaccharide	168:182	arg1	DLODP					223:227	DLODP	223:227	DLODP	223:227	We reported an oligosaccharide diphosphodolichol (DLO) diphosphatase (DLODP) that generates dolichyl-phosphate and oligosaccharyl phosphates (OSPs) from DLO in vitro.
27281477	1	1	theme	oligosaccharide	168:182	arg1	diphosphatase					208:220	an oligosaccharide diphosphodolichol (DLO) diphosphatase	165:220	an oligosaccharide diphosphodolichol (DLO) diphosphatase (DLODP) that generates dolichyl-phosphate and oligosaccharyl phosphates (OSPs) from DLO in vitro	165:317	We reported an oligosaccharide diphosphodolichol (DLO) diphosphatase (DLODP) that generates dolichyl-phosphate and oligosaccharyl phosphates (OSPs) from DLO in vitro.
27281477	8	2	from	GA-	1463:1465	arg1	redistribution					1425:1438	partial redistribution	1417:1438	partial redistribution of DLODP activity from GA- to ER-containing regions of density gradients	1417:1511	The BFA-provoked increase in endomembrane-situated OSP is sensitive to nocodazole, and BFA causes partial redistribution of DLODP activity from GA- to ER-containing regions of density gradients.
27281477	9	3	theme	reported	1619:1626	arg1	DLODP					1628:1632	a previously reported DLODP	1606:1632	a previously reported DLODP that acts to generate a novel endomembrane-situated OSP population	1606:1699	These findings are consistent with BFA-provoked microtubule-dependent GA-to-ER transport of a previously reported DLODP that acts to generate a novel endomembrane-situated OSP population.
27281477	7	4	theme	Man7GlcNAc2-PP-dolichol	1265:1287	arg1	levels					1255:1260	levels	1255:1260	levels of Man7GlcNAc2-PP-dolichol or cytoplasmic Man7GlcNAc2-P	1255:1316	BFA also strikingly increased OSP derived from mature DLO within the endomembrane system without affecting levels of Man7GlcNAc2-PP-dolichol or cytoplasmic Man7GlcNAc2-P.
27281477	1	5	theme	diphosphodolichol	184:200	arg1	DLODP					223:227	DLODP	223:227	DLODP	223:227	We reported an oligosaccharide diphosphodolichol (DLO) diphosphatase (DLODP) that generates dolichyl-phosphate and oligosaccharyl phosphates (OSPs) from DLO in vitro.
27281477	1	5	theme	diphosphodolichol	184:200	arg1	diphosphatase					208:220	an oligosaccharide diphosphodolichol (DLO) diphosphatase	165:220	an oligosaccharide diphosphodolichol (DLO) diphosphatase (DLODP) that generates dolichyl-phosphate and oligosaccharyl phosphates (OSPs) from DLO in vitro	165:317	We reported an oligosaccharide diphosphodolichol (DLO) diphosphatase (DLODP) that generates dolichyl-phosphate and oligosaccharyl phosphates (OSPs) from DLO in vitro.
27281477	6	6	theme	endomannosidase	993:1007	arg1	trimming					1009:1016	GA endomannosidase trimming	990:1016	GA endomannosidase trimming of Glc3Man9GlcNAc2-PP-dolichol	990:1047	We show that BFA provokes GA endomannosidase trimming of Glc3Man9GlcNAc2-PP-dolichol to yield a Man8GlcNAc2-PP-dolichol structure that does not give rise to cytoplasmic Man8GlcNAc2-P.
27281477	0	7	theme	endomembrane	117:128	arg1	system					130:135	the endomembrane system	113:135	the endomembrane system of HepG2 cells	113:150	Brefeldin A promotes the appearance of oligosaccharyl phosphates derived from Glc3Man9GlcNAc2-PP-dolichol within the endomembrane system of HepG2 cells.
27281477	3	8	theme	apparatus	577:585	arg1	marker					592:597	a Golgi apparatus (GA) marker	569:597	a Golgi apparatus (GA) marker	569:597	However, during subcellular fractionation, DLODP distribution is closer to that of a Golgi apparatus (GA) marker than those of ER markers.
27281477	9	9	theme	endomembrane-situated	1664:1684	arg1	population					1690:1699	a novel endomembrane-situated OSP population	1656:1699	a novel endomembrane-situated OSP population	1656:1699	These findings are consistent with BFA-provoked microtubule-dependent GA-to-ER transport of a previously reported DLODP that acts to generate a novel endomembrane-situated OSP population.
27281477	6	10	theme	GA	990:991	arg1	trimming					1009:1016	GA endomannosidase trimming	990:1016	GA endomannosidase trimming of Glc3Man9GlcNAc2-PP-dolichol	990:1047	We show that BFA provokes GA endomannosidase trimming of Glc3Man9GlcNAc2-PP-dolichol to yield a Man8GlcNAc2-PP-dolichol structure that does not give rise to cytoplasmic Man8GlcNAc2-P.
27281477	9	11	theme	DLODP	1628:1632	arg1	transport					1593:1601	BFA-provoked microtubule-dependent GA-to-ER transport	1549:1601	BFA-provoked microtubule-dependent GA-to-ER transport of a previously reported DLODP that acts to generate a novel endomembrane-situated OSP population	1549:1699	These findings are consistent with BFA-provoked microtubule-dependent GA-to-ER transport of a previously reported DLODP that acts to generate a novel endomembrane-situated OSP population.
27281477	8	12	theme	partial	1417:1423	arg1	redistribution					1425:1438	partial redistribution	1417:1438	partial redistribution of DLODP activity from GA- to ER-containing regions of density gradients	1417:1511	The BFA-provoked increase in endomembrane-situated OSP is sensitive to nocodazole, and BFA causes partial redistribution of DLODP activity from GA- to ER-containing regions of density gradients.
27281477	7	13	attach	derived	1182:1188	arg2	OSP					1178:1180	OSP	1178:1180	OSP derived from mature DLO	1178:1204	BFA also strikingly increased OSP derived from mature DLO within the endomembrane system without affecting levels of Man7GlcNAc2-PP-dolichol or cytoplasmic Man7GlcNAc2-P.
27281477	7	13	attach	derived	1182:1188	arg1	DLO					1202:1204	mature DLO	1195:1204	mature DLO	1195:1204	BFA also strikingly increased OSP derived from mature DLO within the endomembrane system without affecting levels of Man7GlcNAc2-PP-dolichol or cytoplasmic Man7GlcNAc2-P.
27281477	5	14	theme	dolichyl-P-mannose	857:874	arg1	mannosyltransferase					900:918	dolichyl-P-mannose Man7GlcNAc2-PP-dolichol mannosyltransferase	857:918	dolichyl-P-mannose Man7GlcNAc2-PP-dolichol mannosyltransferase	857:918	In order to increase the steady state level of truncated DLO while allowing formation of mature DLO (Glc3Man9GlcNAc2-PP-dolichol), dolichyl-P-mannose Man7GlcNAc2-PP-dolichol mannosyltransferase was partially downregulated in HepG2 cells.
27281477	9	15	theme	microtubule-dependent	1562:1582	arg1	transport					1593:1601	BFA-provoked microtubule-dependent GA-to-ER transport	1549:1601	BFA-provoked microtubule-dependent GA-to-ER transport of a previously reported DLODP that acts to generate a novel endomembrane-situated OSP population	1549:1699	These findings are consistent with BFA-provoked microtubule-dependent GA-to-ER transport of a previously reported DLODP that acts to generate a novel endomembrane-situated OSP population.
27281477	5	16	theme	mature	815:820	arg1	DLO					822:824	mature DLO	815:824	mature DLO (Glc3Man9GlcNAc2-PP-dolichol)	815:854	In order to increase the steady state level of truncated DLO while allowing formation of mature DLO (Glc3Man9GlcNAc2-PP-dolichol), dolichyl-P-mannose Man7GlcNAc2-PP-dolichol mannosyltransferase was partially downregulated in HepG2 cells.
27281477	5	16	theme	mature	815:820	arg1	Glc3Man9GlcNAc2-PP-dolichol					827:853	Glc3Man9GlcNAc2-PP-dolichol	827:853	Glc3Man9GlcNAc2-PP-dolichol	827:853	In order to increase the steady state level of truncated DLO while allowing formation of mature DLO (Glc3Man9GlcNAc2-PP-dolichol), dolichyl-P-mannose Man7GlcNAc2-PP-dolichol mannosyltransferase was partially downregulated in HepG2 cells.
27281477	9	17	with	consistent	1533:1542	arg1	transport					1593:1601	BFA-provoked microtubule-dependent GA-to-ER transport	1549:1601	BFA-provoked microtubule-dependent GA-to-ER transport of a previously reported DLODP that acts to generate a novel endomembrane-situated OSP population	1549:1699	These findings are consistent with BFA-provoked microtubule-dependent GA-to-ER transport of a previously reported DLODP that acts to generate a novel endomembrane-situated OSP population.
27281477	6	18	theme	cytoplasmic	1121:1131	arg1	Man8GlcNAc2-P					1133:1145	cytoplasmic Man8GlcNAc2-P	1121:1145	cytoplasmic Man8GlcNAc2-P	1121:1145	We show that BFA provokes GA endomannosidase trimming of Glc3Man9GlcNAc2-PP-dolichol to yield a Man8GlcNAc2-PP-dolichol structure that does not give rise to cytoplasmic Man8GlcNAc2-P.
27281477	8	19	theme	DLODP	1443:1447	arg1	activity					1449:1456	DLODP activity	1443:1456	DLODP activity	1443:1456	The BFA-provoked increase in endomembrane-situated OSP is sensitive to nocodazole, and BFA causes partial redistribution of DLODP activity from GA- to ER-containing regions of density gradients.
27281477	8	20	theme	BFA-provoked	1323:1334	arg1	sensitive					1377:1385	sensitive	1377:1385	sensitive	1377:1385	The BFA-provoked increase in endomembrane-situated OSP is sensitive to nocodazole, and BFA causes partial redistribution of DLODP activity from GA- to ER-containing regions of density gradients.
27281477	8	20	theme	BFA-provoked	1323:1334	arg1	increase					1336:1343	The BFA-provoked increase	1319:1343	The BFA-provoked increase in endomembrane-situated OSP	1319:1372	The BFA-provoked increase in endomembrane-situated OSP is sensitive to nocodazole, and BFA causes partial redistribution of DLODP activity from GA- to ER-containing regions of density gradients.
27281477	2	21	theme	reticulum	442:450	arg1	intermediates					471:483	truncated endoplasmic reticulum (ER)-generated DLO intermediates	420:483	truncated endoplasmic reticulum (ER)-generated DLO intermediates	420:483	This enzyme could underlie cytoplasmic OSP generation and promote dolichyl-phosphate recycling from truncated endoplasmic reticulum (ER)-generated DLO intermediates.
27281477	5	22	theme	state	758:762	arg1	level					764:768	the steady state level	747:768	the steady state level of truncated DLO	747:785	In order to increase the steady state level of truncated DLO while allowing formation of mature DLO (Glc3Man9GlcNAc2-PP-dolichol), dolichyl-P-mannose Man7GlcNAc2-PP-dolichol mannosyltransferase was partially downregulated in HepG2 cells.
27281477	5	23	theme	Man7GlcNAc2-PP-dolichol	876:898	arg1	mannosyltransferase					900:918	dolichyl-P-mannose Man7GlcNAc2-PP-dolichol mannosyltransferase	857:918	dolichyl-P-mannose Man7GlcNAc2-PP-dolichol mannosyltransferase	857:918	In order to increase the steady state level of truncated DLO while allowing formation of mature DLO (Glc3Man9GlcNAc2-PP-dolichol), dolichyl-P-mannose Man7GlcNAc2-PP-dolichol mannosyltransferase was partially downregulated in HepG2 cells.
27281477	3	24	theme	Golgi	571:575	arg1	marker					592:597	a Golgi apparatus (GA) marker	569:597	a Golgi apparatus (GA) marker	569:597	However, during subcellular fractionation, DLODP distribution is closer to that of a Golgi apparatus (GA) marker than those of ER markers.
27281477	1	25	theme	DLO	203:205	arg1	DLODP					223:227	DLODP	223:227	DLODP	223:227	We reported an oligosaccharide diphosphodolichol (DLO) diphosphatase (DLODP) that generates dolichyl-phosphate and oligosaccharyl phosphates (OSPs) from DLO in vitro.
27281477	1	25	theme	DLO	203:205	arg1	diphosphatase					208:220	an oligosaccharide diphosphodolichol (DLO) diphosphatase	165:220	an oligosaccharide diphosphodolichol (DLO) diphosphatase (DLODP) that generates dolichyl-phosphate and oligosaccharyl phosphates (OSPs) from DLO in vitro	165:317	We reported an oligosaccharide diphosphodolichol (DLO) diphosphatase (DLODP) that generates dolichyl-phosphate and oligosaccharyl phosphates (OSPs) from DLO in vitro.
27281477	4	26	theme	brefeldin	657:665	arg1	A					667:667	brefeldin A	657:667	brefeldin A (BFA)	657:673	Here, we examined the effect of brefeldin A (BFA), which fuses the GA with the ER on OSP metabolism.
27281477	4	26	theme	brefeldin	657:665	arg1	BFA					670:672	BFA	670:672	BFA	670:672	Here, we examined the effect of brefeldin A (BFA), which fuses the GA with the ER on OSP metabolism.
27281477	2	27	theme	truncated	420:428	arg1	intermediates					471:483	truncated endoplasmic reticulum (ER)-generated DLO intermediates	420:483	truncated endoplasmic reticulum (ER)-generated DLO intermediates	420:483	This enzyme could underlie cytoplasmic OSP generation and promote dolichyl-phosphate recycling from truncated endoplasmic reticulum (ER)-generated DLO intermediates.
27281477	0	28	theme	Brefeldin	0:8	arg1	A					10:10	Brefeldin A	0:10	Brefeldin A	0:10	Brefeldin A promotes the appearance of oligosaccharyl phosphates derived from Glc3Man9GlcNAc2-PP-dolichol within the endomembrane system of HepG2 cells.
27281477	1	29	from	DLO	306:308	arg1	OSPs					295:298	OSPs	295:298	OSPs	295:298	We reported an oligosaccharide diphosphodolichol (DLO) diphosphatase (DLODP) that generates dolichyl-phosphate and oligosaccharyl phosphates (OSPs) from DLO in vitro.
27281477	1	29	from	DLO	306:308	arg1	phosphates					283:292	dolichyl-phosphate and oligosaccharyl phosphates	245:292	dolichyl-phosphate and oligosaccharyl phosphates (OSPs) from DLO	245:308	We reported an oligosaccharide diphosphodolichol (DLO) diphosphatase (DLODP) that generates dolichyl-phosphate and oligosaccharyl phosphates (OSPs) from DLO in vitro.
27281477	5	30	theme	HepG2	951:955	arg1	cells					957:961	HepG2 cells	951:961	HepG2 cells	951:961	In order to increase the steady state level of truncated DLO while allowing formation of mature DLO (Glc3Man9GlcNAc2-PP-dolichol), dolichyl-P-mannose Man7GlcNAc2-PP-dolichol mannosyltransferase was partially downregulated in HepG2 cells.
27281477	9	31	theme	BFA-provoked	1549:1560	arg1	transport					1593:1601	BFA-provoked microtubule-dependent GA-to-ER transport	1549:1601	BFA-provoked microtubule-dependent GA-to-ER transport of a previously reported DLODP that acts to generate a novel endomembrane-situated OSP population	1549:1699	These findings are consistent with BFA-provoked microtubule-dependent GA-to-ER transport of a previously reported DLODP that acts to generate a novel endomembrane-situated OSP population.
27281477	5	32	theme	truncated	773:781	arg1	DLO					783:785	truncated DLO	773:785	truncated DLO	773:785	In order to increase the steady state level of truncated DLO while allowing formation of mature DLO (Glc3Man9GlcNAc2-PP-dolichol), dolichyl-P-mannose Man7GlcNAc2-PP-dolichol mannosyltransferase was partially downregulated in HepG2 cells.
27281477	0	33	theme	cells	146:150	arg1	system					130:135	the endomembrane system	113:135	the endomembrane system of HepG2 cells	113:150	Brefeldin A promotes the appearance of oligosaccharyl phosphates derived from Glc3Man9GlcNAc2-PP-dolichol within the endomembrane system of HepG2 cells.
27281477	3	34	theme	GA	588:589	arg1	marker					592:597	a Golgi apparatus (GA) marker	569:597	a Golgi apparatus (GA) marker	569:597	However, during subcellular fractionation, DLODP distribution is closer to that of a Golgi apparatus (GA) marker than those of ER markers.
27281477	4	35	from	effect	647:652	arg1	metabolism					714:723	OSP metabolism	710:723	OSP metabolism	710:723	Here, we examined the effect of brefeldin A (BFA), which fuses the GA with the ER on OSP metabolism.
27281477	2	36	theme	-generated	456:465	arg1	intermediates					471:483	truncated endoplasmic reticulum (ER)-generated DLO intermediates	420:483	truncated endoplasmic reticulum (ER)-generated DLO intermediates	420:483	This enzyme could underlie cytoplasmic OSP generation and promote dolichyl-phosphate recycling from truncated endoplasmic reticulum (ER)-generated DLO intermediates.
27281477	2	37	theme	DLO	467:469	arg1	intermediates					471:483	truncated endoplasmic reticulum (ER)-generated DLO intermediates	420:483	truncated endoplasmic reticulum (ER)-generated DLO intermediates	420:483	This enzyme could underlie cytoplasmic OSP generation and promote dolichyl-phosphate recycling from truncated endoplasmic reticulum (ER)-generated DLO intermediates.
27281477	0	38	theme	HepG2	140:144	arg1	cells					146:150	HepG2 cells	140:150	HepG2 cells	140:150	Brefeldin A promotes the appearance of oligosaccharyl phosphates derived from Glc3Man9GlcNAc2-PP-dolichol within the endomembrane system of HepG2 cells.
27281477	4	39	theme	OSP	710:712	arg1	metabolism					714:723	OSP metabolism	710:723	OSP metabolism	710:723	Here, we examined the effect of brefeldin A (BFA), which fuses the GA with the ER on OSP metabolism.
27281477	5	40	theme	DLO	822:824	arg1	formation					802:810	formation	802:810	formation of mature DLO (Glc3Man9GlcNAc2-PP-dolichol)	802:854	In order to increase the steady state level of truncated DLO while allowing formation of mature DLO (Glc3Man9GlcNAc2-PP-dolichol), dolichyl-P-mannose Man7GlcNAc2-PP-dolichol mannosyltransferase was partially downregulated in HepG2 cells.
27281477	7	41	theme	endomembrane	1217:1228	arg1	system					1230:1235	the endomembrane system	1213:1235	the endomembrane system	1213:1235	BFA also strikingly increased OSP derived from mature DLO within the endomembrane system without affecting levels of Man7GlcNAc2-PP-dolichol or cytoplasmic Man7GlcNAc2-P.
27281477	3	42	theme	ER	613:614	arg1	markers					616:622	ER markers	613:622	ER markers	613:622	However, during subcellular fractionation, DLODP distribution is closer to that of a Golgi apparatus (GA) marker than those of ER markers.
27281477	6	43	theme	Man8GlcNAc2-PP-dolichol	1060:1082	arg1	structure					1084:1092	a Man8GlcNAc2-PP-dolichol structure	1058:1092	a Man8GlcNAc2-PP-dolichol structure that does not give rise to cytoplasmic Man8GlcNAc2-P	1058:1145	We show that BFA provokes GA endomannosidase trimming of Glc3Man9GlcNAc2-PP-dolichol to yield a Man8GlcNAc2-PP-dolichol structure that does not give rise to cytoplasmic Man8GlcNAc2-P.
27281477	4	44	theme	A	667:667	arg1	effect					647:652	the effect	643:652	the effect of brefeldin A (BFA), which fuses the GA with the ER on OSP metabolism	643:723	Here, we examined the effect of brefeldin A (BFA), which fuses the GA with the ER on OSP metabolism.
27281477	7	45	theme	mature	1195:1200	arg1	DLO					1202:1204	mature DLO	1195:1204	mature DLO	1195:1204	BFA also strikingly increased OSP derived from mature DLO within the endomembrane system without affecting levels of Man7GlcNAc2-PP-dolichol or cytoplasmic Man7GlcNAc2-P.
27281477	8	46	theme	ER-containing	1470:1482	arg1	regions					1484:1490	ER-containing regions	1470:1490	ER-containing regions of density gradients	1470:1511	The BFA-provoked increase in endomembrane-situated OSP is sensitive to nocodazole, and BFA causes partial redistribution of DLODP activity from GA- to ER-containing regions of density gradients.
27281477	0	47	attach	derived	65:71	arg2	phosphates					54:63	oligosaccharyl phosphates	39:63	oligosaccharyl phosphates derived from Glc3Man9GlcNAc2-PP-dolichol within the endomembrane system of HepG2 cells	39:150	Brefeldin A promotes the appearance of oligosaccharyl phosphates derived from Glc3Man9GlcNAc2-PP-dolichol within the endomembrane system of HepG2 cells.
27281477	0	47	attach	derived	65:71	arg1	Glc3Man9GlcNAc2-PP-dolichol					78:104	Glc3Man9GlcNAc2-PP-dolichol	78:104	Glc3Man9GlcNAc2-PP-dolichol within the endomembrane system of HepG2 cells	78:150	Brefeldin A promotes the appearance of oligosaccharyl phosphates derived from Glc3Man9GlcNAc2-PP-dolichol within the endomembrane system of HepG2 cells.
27281477	8	48	theme	endomembrane-situated	1348:1368	arg1	OSP					1370:1372	endomembrane-situated OSP	1348:1372	endomembrane-situated OSP	1348:1372	The BFA-provoked increase in endomembrane-situated OSP is sensitive to nocodazole, and BFA causes partial redistribution of DLODP activity from GA- to ER-containing regions of density gradients.
27281477	0	49	theme	phosphates	54:63	arg1	appearance					25:34	the appearance	21:34	the appearance of oligosaccharyl phosphates derived from Glc3Man9GlcNAc2-PP-dolichol within the endomembrane system of HepG2 cells	21:150	Brefeldin A promotes the appearance of oligosaccharyl phosphates derived from Glc3Man9GlcNAc2-PP-dolichol within the endomembrane system of HepG2 cells.
27281477	7	50	theme	cytoplasmic	1292:1302	arg1	Man7GlcNAc2-P					1304:1316	cytoplasmic Man7GlcNAc2-P	1292:1316	cytoplasmic Man7GlcNAc2-P	1292:1316	BFA also strikingly increased OSP derived from mature DLO within the endomembrane system without affecting levels of Man7GlcNAc2-PP-dolichol or cytoplasmic Man7GlcNAc2-P.
27281477	8	51	theme	density	1495:1501	arg1	gradients					1503:1511	density gradients	1495:1511	density gradients	1495:1511	The BFA-provoked increase in endomembrane-situated OSP is sensitive to nocodazole, and BFA causes partial redistribution of DLODP activity from GA- to ER-containing regions of density gradients.
27281477	8	52	theme	activity	1449:1456	arg1	redistribution					1425:1438	partial redistribution	1417:1438	partial redistribution of DLODP activity from GA- to ER-containing regions of density gradients	1417:1511	The BFA-provoked increase in endomembrane-situated OSP is sensitive to nocodazole, and BFA causes partial redistribution of DLODP activity from GA- to ER-containing regions of density gradients.
27281477	0	53	theme	oligosaccharyl	39:52	arg1	phosphates					54:63	oligosaccharyl phosphates	39:63	oligosaccharyl phosphates derived from Glc3Man9GlcNAc2-PP-dolichol within the endomembrane system of HepG2 cells	39:150	Brefeldin A promotes the appearance of oligosaccharyl phosphates derived from Glc3Man9GlcNAc2-PP-dolichol within the endomembrane system of HepG2 cells.
27281477	9	54	theme	GA-to-ER	1584:1591	arg1	transport					1593:1601	BFA-provoked microtubule-dependent GA-to-ER transport	1549:1601	BFA-provoked microtubule-dependent GA-to-ER transport of a previously reported DLODP that acts to generate a novel endomembrane-situated OSP population	1549:1699	These findings are consistent with BFA-provoked microtubule-dependent GA-to-ER transport of a previously reported DLODP that acts to generate a novel endomembrane-situated OSP population.
27281477	2	55	theme	OSP	359:361	arg1	generation					363:372	cytoplasmic OSP generation	347:372	cytoplasmic OSP generation	347:372	This enzyme could underlie cytoplasmic OSP generation and promote dolichyl-phosphate recycling from truncated endoplasmic reticulum (ER)-generated DLO intermediates.
27281477	9	56	theme	novel	1658:1662	arg1	population					1690:1699	a novel endomembrane-situated OSP population	1656:1699	a novel endomembrane-situated OSP population	1656:1699	These findings are consistent with BFA-provoked microtubule-dependent GA-to-ER transport of a previously reported DLODP that acts to generate a novel endomembrane-situated OSP population.
27281477	2	57	theme	cytoplasmic	347:357	arg1	generation					363:372	cytoplasmic OSP generation	347:372	cytoplasmic OSP generation	347:372	This enzyme could underlie cytoplasmic OSP generation and promote dolichyl-phosphate recycling from truncated endoplasmic reticulum (ER)-generated DLO intermediates.
27281477	9	58	theme	OSP	1686:1688	arg1	population					1690:1699	a novel endomembrane-situated OSP population	1656:1699	a novel endomembrane-situated OSP population	1656:1699	These findings are consistent with BFA-provoked microtubule-dependent GA-to-ER transport of a previously reported DLODP that acts to generate a novel endomembrane-situated OSP population.
27281477	8	59	theme	gradients	1503:1511	arg1	regions					1484:1490	ER-containing regions	1470:1490	ER-containing regions of density gradients	1470:1511	The BFA-provoked increase in endomembrane-situated OSP is sensitive to nocodazole, and BFA causes partial redistribution of DLODP activity from GA- to ER-containing regions of density gradients.
27281477	3	60	theme	DLODP	529:533	arg1	distribution					535:546	DLODP distribution	529:546	DLODP distribution	529:546	However, during subcellular fractionation, DLODP distribution is closer to that of a Golgi apparatus (GA) marker than those of ER markers.
27281477	1	61	theme	dolichyl-phosphate	245:262	arg1	OSPs					295:298	OSPs	295:298	OSPs	295:298	We reported an oligosaccharide diphosphodolichol (DLO) diphosphatase (DLODP) that generates dolichyl-phosphate and oligosaccharyl phosphates (OSPs) from DLO in vitro.
27281477	1	61	theme	dolichyl-phosphate	245:262	arg1	phosphates					283:292	dolichyl-phosphate and oligosaccharyl phosphates	245:292	dolichyl-phosphate and oligosaccharyl phosphates (OSPs) from DLO	245:308	We reported an oligosaccharide diphosphodolichol (DLO) diphosphatase (DLODP) that generates dolichyl-phosphate and oligosaccharyl phosphates (OSPs) from DLO in vitro.
27281477	3	62	theme	subcellular	502:512	arg1	fractionation					514:526	subcellular fractionation	502:526	subcellular fractionation	502:526	However, during subcellular fractionation, DLODP distribution is closer to that of a Golgi apparatus (GA) marker than those of ER markers.
27281477	2	63	theme	endoplasmic	430:440	arg1	ER					453:454	ER	453:454	ER	453:454	This enzyme could underlie cytoplasmic OSP generation and promote dolichyl-phosphate recycling from truncated endoplasmic reticulum (ER)-generated DLO intermediates.
27281477	2	63	theme	endoplasmic	430:440	arg1	reticulum					442:450	endoplasmic reticulum	430:450	truncated endoplasmic reticulum (ER)-generated DLO intermediates	420:483	This enzyme could underlie cytoplasmic OSP generation and promote dolichyl-phosphate recycling from truncated endoplasmic reticulum (ER)-generated DLO intermediates.
27281477	6	64	theme	Glc3Man9GlcNAc2-PP-dolichol	1021:1047	arg1	trimming					1009:1016	GA endomannosidase trimming	990:1016	GA endomannosidase trimming of Glc3Man9GlcNAc2-PP-dolichol	990:1047	We show that BFA provokes GA endomannosidase trimming of Glc3Man9GlcNAc2-PP-dolichol to yield a Man8GlcNAc2-PP-dolichol structure that does not give rise to cytoplasmic Man8GlcNAc2-P.
27281477	5	65	theme	steady	751:756	arg1	level					764:768	the steady state level	747:768	the steady state level of truncated DLO	747:785	In order to increase the steady state level of truncated DLO while allowing formation of mature DLO (Glc3Man9GlcNAc2-PP-dolichol), dolichyl-P-mannose Man7GlcNAc2-PP-dolichol mannosyltransferase was partially downregulated in HepG2 cells.
27281477	2	66	from	intermediates	471:483	arg1	recycling					405:413	dolichyl-phosphate recycling	386:413	dolichyl-phosphate recycling from truncated endoplasmic reticulum (ER)-generated DLO intermediates	386:483	This enzyme could underlie cytoplasmic OSP generation and promote dolichyl-phosphate recycling from truncated endoplasmic reticulum (ER)-generated DLO intermediates.
27281477	5	67	theme	DLO	783:785	arg1	level					764:768	the steady state level	747:768	the steady state level of truncated DLO	747:785	In order to increase the steady state level of truncated DLO while allowing formation of mature DLO (Glc3Man9GlcNAc2-PP-dolichol), dolichyl-P-mannose Man7GlcNAc2-PP-dolichol mannosyltransferase was partially downregulated in HepG2 cells.
27281477	2	68	theme	dolichyl-phosphate	386:403	arg1	recycling					405:413	dolichyl-phosphate recycling	386:413	dolichyl-phosphate recycling from truncated endoplasmic reticulum (ER)-generated DLO intermediates	386:483	This enzyme could underlie cytoplasmic OSP generation and promote dolichyl-phosphate recycling from truncated endoplasmic reticulum (ER)-generated DLO intermediates.
27281477	7	69	theme	Man7GlcNAc2-P	1304:1316	arg1	levels					1255:1260	levels	1255:1260	levels of Man7GlcNAc2-PP-dolichol or cytoplasmic Man7GlcNAc2-P	1255:1316	BFA also strikingly increased OSP derived from mature DLO within the endomembrane system without affecting levels of Man7GlcNAc2-PP-dolichol or cytoplasmic Man7GlcNAc2-P.
27281477	1	70	theme	oligosaccharyl	268:281	arg1	OSPs					295:298	OSPs	295:298	OSPs	295:298	We reported an oligosaccharide diphosphodolichol (DLO) diphosphatase (DLODP) that generates dolichyl-phosphate and oligosaccharyl phosphates (OSPs) from DLO in vitro.
27281477	1	70	theme	oligosaccharyl	268:281	arg1	phosphates					283:292	dolichyl-phosphate and oligosaccharyl phosphates	245:292	dolichyl-phosphate and oligosaccharyl phosphates (OSPs) from DLO	245:308	We reported an oligosaccharide diphosphodolichol (DLO) diphosphatase (DLODP) that generates dolichyl-phosphate and oligosaccharyl phosphates (OSPs) from DLO in vitro.
25129803	5	0	theme	%	683:683	arg1	degree					664:669	a crosslinking degree	649:669	a crosslinking degree of around 90%	649:683	The obtained hydrogel, with a crosslinking degree of around 90%, showed interesting swelling properties.
25129803	2	1	theme	di-alkyne	325:333	arg1	PEO					335:337	a di-alkyne PEO	323:337	a di-alkyne PEO by a regioselective Cu(I)-catalyzed cycloaddition	323:387	Azide functionalities were introduced onto chitosan, through mesylation of C-6 hydroxyl groups, and reacted with a di-alkyne PEO by a regioselective Cu(I)-catalyzed cycloaddition.
25129803	4	2	from	amount	546:551	arg1	chitosan					572:579	chitosan	572:579	chitosan	572:579	In fact, the extent of coupling is strictly dependent on the amount of azido groups on chitosan, which in turn can be easily modulated.
25129803	7	3	theme	controlled	1030:1039	arg1	release					1046:1052	controlled drug release	1030:1052	controlled drug release	1030:1052	The possibility to control the crosslinking degree of hydrogel and its capability to rapidly absorb high amounts of water make this material suitable for several applications, such as controlled drug release and wound healing.
25129803	3	4	theme	crosslinking	464:475	arg1	degree					477:482	a controlled crosslinking degree	451:482	a controlled crosslinking degree	451:482	This synthetic approach allowed us to obtain a hydrogel with a controlled crosslinking degree.
25129803	6	5	dep	reached	830:836	arg1	%					842:842	940%	839:842	940%	839:842	With respect to chitosan hydrogels reported in literature, a considerably higher equilibrium uptake was reached (940%).
25129803	7	6	theme	crosslinking	877:888	arg1	degree					890:895	the crosslinking degree	873:895	the crosslinking degree of hydrogel	873:907	The possibility to control the crosslinking degree of hydrogel and its capability to rapidly absorb high amounts of water make this material suitable for several applications, such as controlled drug release and wound healing.
25129803	5	7	theme	swelling	705:712	arg1	properties					714:723	interesting swelling properties	693:723	interesting swelling properties	693:723	The obtained hydrogel, with a crosslinking degree of around 90%, showed interesting swelling properties.
25129803	0	8	theme	-catalyzed	75:84	arg1	cycloaddition					86:98	regioselective Cu(I)-catalyzed cycloaddition	55:98	regioselective Cu(I)-catalyzed cycloaddition	55:98	Synthesis of chitosan-PEO hydrogels via mesylation and regioselective Cu(I)-catalyzed cycloaddition.
25129803	3	9	theme	controlled	453:462	arg1	degree					477:482	a controlled crosslinking degree	451:482	a controlled crosslinking degree	451:482	This synthetic approach allowed us to obtain a hydrogel with a controlled crosslinking degree.
25129803	4	10	from	dependent	529:537	arg1	fact					488:491	fact	488:491	fact	488:491	In fact, the extent of coupling is strictly dependent on the amount of azido groups on chitosan, which in turn can be easily modulated.
25129803	7	11	theme	several	1000:1006	arg1	applications					1008:1019	several applications	1000:1019	several applications	1000:1019	The possibility to control the crosslinking degree of hydrogel and its capability to rapidly absorb high amounts of water make this material suitable for several applications, such as controlled drug release and wound healing.
25129803	7	11	theme	several	1000:1006	arg1	release					1046:1052	controlled drug release	1030:1052	controlled drug release	1030:1052	The possibility to control the crosslinking degree of hydrogel and its capability to rapidly absorb high amounts of water make this material suitable for several applications, such as controlled drug release and wound healing.
25129803	7	11	theme	several	1000:1006	arg1	healing					1064:1070	wound healing	1058:1070	wound healing	1058:1070	The possibility to control the crosslinking degree of hydrogel and its capability to rapidly absorb high amounts of water make this material suitable for several applications, such as controlled drug release and wound healing.
25129803	7	12	dep	capability	917:926	arg1	absorb					939:944	absorb	939:944	to rapidly absorb high amounts of water	928:966	The possibility to control the crosslinking degree of hydrogel and its capability to rapidly absorb high amounts of water make this material suitable for several applications, such as controlled drug release and wound healing.
25129803	3	13	theme	synthetic	395:403	arg1	approach					405:412	This synthetic approach	390:412	This synthetic approach	390:412	This synthetic approach allowed us to obtain a hydrogel with a controlled crosslinking degree.
25129803	6	14	theme	equilibrium	807:817	arg1	uptake					819:824	a considerably higher equilibrium uptake	785:824	a considerably higher equilibrium uptake	785:824	With respect to chitosan hydrogels reported in literature, a considerably higher equilibrium uptake was reached (940%).
25129803	3	15	with	hydrogel	437:444	arg1	degree					477:482	a controlled crosslinking degree	451:482	a controlled crosslinking degree	451:482	This synthetic approach allowed us to obtain a hydrogel with a controlled crosslinking degree.
25129803	1	16	theme	coupling	156:163	arg1	chitosan					165:172	coupling chitosan	156:172	coupling chitosan with PEO through "click chemistry"	156:207	In this work, a well-defined hydrogel was developed by coupling chitosan with PEO through "click chemistry".
25129803	6	17	theme	higher	800:805	arg1	uptake					819:824	a considerably higher equilibrium uptake	785:824	a considerably higher equilibrium uptake	785:824	With respect to chitosan hydrogels reported in literature, a considerably higher equilibrium uptake was reached (940%).
25129803	2	18	theme	groups	298:303	arg1	mesylation					271:280	mesylation	271:280	mesylation of C-6 hydroxyl groups	271:303	Azide functionalities were introduced onto chitosan, through mesylation of C-6 hydroxyl groups, and reacted with a di-alkyne PEO by a regioselective Cu(I)-catalyzed cycloaddition.
25129803	6	19	dep	hydrogels	751:759	arg1	respect					731:737	respect	731:737	respect	731:737	With respect to chitosan hydrogels reported in literature, a considerably higher equilibrium uptake was reached (940%).
25129803	6	20	theme	chitosan	742:749	arg1	hydrogels					751:759	chitosan hydrogels	742:759	chitosan hydrogels reported in literature	742:782	With respect to chitosan hydrogels reported in literature, a considerably higher equilibrium uptake was reached (940%).
25129803	2	21	theme	hydroxyl	289:296	arg1	groups					298:303	C-6 hydroxyl groups	285:303	C-6 hydroxyl groups	285:303	Azide functionalities were introduced onto chitosan, through mesylation of C-6 hydroxyl groups, and reacted with a di-alkyne PEO by a regioselective Cu(I)-catalyzed cycloaddition.
25129803	5	22	theme	obtained	625:632	arg1	hydrogel					634:641	The obtained hydrogel	621:641	The obtained hydrogel	621:641	The obtained hydrogel, with a crosslinking degree of around 90%, showed interesting swelling properties.
25129803	2	23	theme	C-6	285:287	arg1	groups					298:303	C-6 hydroxyl groups	285:303	C-6 hydroxyl groups	285:303	Azide functionalities were introduced onto chitosan, through mesylation of C-6 hydroxyl groups, and reacted with a di-alkyne PEO by a regioselective Cu(I)-catalyzed cycloaddition.
25129803	0	24	theme	chitosan-PEO	13:24	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of chitosan-PEO	0:24	Synthesis of chitosan-PEO hydrogels via mesylation and regioselective Cu(I)-catalyzed cycloaddition.
25129803	4	25	from	chitosan	572:579	arg1	amount					546:551	the amount	542:551	the amount	542:551	In fact, the extent of coupling is strictly dependent on the amount of azido groups on chitosan, which in turn can be easily modulated.
25129803	4	25	from	chitosan	572:579	arg1	groups					562:567	azido groups	556:567	azido groups on chitosan	556:579	In fact, the extent of coupling is strictly dependent on the amount of azido groups on chitosan, which in turn can be easily modulated.
25129803	2	26	theme	-catalyzed	364:373	arg1	cycloaddition					375:387	a regioselective Cu(I)-catalyzed cycloaddition	342:387	a regioselective Cu(I)-catalyzed cycloaddition	342:387	Azide functionalities were introduced onto chitosan, through mesylation of C-6 hydroxyl groups, and reacted with a di-alkyne PEO by a regioselective Cu(I)-catalyzed cycloaddition.
25129803	4	27	theme	coupling	508:515	arg1	dependent					529:537	dependent	529:537	dependent	529:537	In fact, the extent of coupling is strictly dependent on the amount of azido groups on chitosan, which in turn can be easily modulated.
25129803	4	27	theme	coupling	508:515	arg1	extent					498:503	the extent	494:503	the extent of coupling	494:515	In fact, the extent of coupling is strictly dependent on the amount of azido groups on chitosan, which in turn can be easily modulated.
25129803	7	28	theme	water	962:966	arg1	amounts					951:957	high amounts	946:957	high amounts of water	946:966	The possibility to control the crosslinking degree of hydrogel and its capability to rapidly absorb high amounts of water make this material suitable for several applications, such as controlled drug release and wound healing.
25129803	7	28	theme	water	962:966	arg1	water					962:966	water	962:966	water	962:966	The possibility to control the crosslinking degree of hydrogel and its capability to rapidly absorb high amounts of water make this material suitable for several applications, such as controlled drug release and wound healing.
25129803	2	29	theme	Azide	210:214	arg1	functionalities					216:230	Azide functionalities	210:230	Azide functionalities	210:230	Azide functionalities were introduced onto chitosan, through mesylation of C-6 hydroxyl groups, and reacted with a di-alkyne PEO by a regioselective Cu(I)-catalyzed cycloaddition.
25129803	7	30	theme	drug	1041:1044	arg1	release					1046:1052	controlled drug release	1030:1052	controlled drug release	1030:1052	The possibility to control the crosslinking degree of hydrogel and its capability to rapidly absorb high amounts of water make this material suitable for several applications, such as controlled drug release and wound healing.
25129803	5	31	theme	interesting	693:703	arg1	properties					714:723	interesting swelling properties	693:723	interesting swelling properties	693:723	The obtained hydrogel, with a crosslinking degree of around 90%, showed interesting swelling properties.
25129803	7	32	theme	wound	1058:1062	arg1	healing					1064:1070	wound healing	1058:1070	wound healing	1058:1070	The possibility to control the crosslinking degree of hydrogel and its capability to rapidly absorb high amounts of water make this material suitable for several applications, such as controlled drug release and wound healing.
25129803	5	33	theme	crosslinking	651:662	arg1	degree					664:669	a crosslinking degree	649:669	a crosslinking degree of around 90%	649:683	The obtained hydrogel, with a crosslinking degree of around 90%, showed interesting swelling properties.
25129803	1	34	theme	click	192:196	arg1	"					207:207	"click chemistry"	191:207	"click chemistry"	191:207	In this work, a well-defined hydrogel was developed by coupling chitosan with PEO through "click chemistry".
25129803	0	35	theme	regioselective	55:68	arg1	cycloaddition					86:98	regioselective Cu(I)-catalyzed cycloaddition	55:98	regioselective Cu(I)-catalyzed cycloaddition	55:98	Synthesis of chitosan-PEO hydrogels via mesylation and regioselective Cu(I)-catalyzed cycloaddition.
25129803	1	36	with	chitosan	165:172	arg1	PEO					179:181	PEO	179:181	PEO	179:181	In this work, a well-defined hydrogel was developed by coupling chitosan with PEO through "click chemistry".
25129803	1	37	theme	chemistry	198:206	arg1	"					207:207	"click chemistry"	191:207	"click chemistry"	191:207	In this work, a well-defined hydrogel was developed by coupling chitosan with PEO through "click chemistry".
25129803	7	38	theme	high	946:949	arg1	amounts					951:957	high amounts	946:957	high amounts of water	946:966	The possibility to control the crosslinking degree of hydrogel and its capability to rapidly absorb high amounts of water make this material suitable for several applications, such as controlled drug release and wound healing.
25129803	7	38	theme	high	946:949	arg1	water					962:966	water	962:966	water	962:966	The possibility to control the crosslinking degree of hydrogel and its capability to rapidly absorb high amounts of water make this material suitable for several applications, such as controlled drug release and wound healing.
25129803	7	39	theme	hydrogel	900:907	arg1	degree					890:895	the crosslinking degree	873:895	the crosslinking degree of hydrogel	873:907	The possibility to control the crosslinking degree of hydrogel and its capability to rapidly absorb high amounts of water make this material suitable for several applications, such as controlled drug release and wound healing.
25129803	4	40	from	groups	562:567	arg1	chitosan					572:579	chitosan	572:579	chitosan	572:579	In fact, the extent of coupling is strictly dependent on the amount of azido groups on chitosan, which in turn can be easily modulated.
25129803	5	41	with	hydrogel	634:641	arg1	degree					664:669	a crosslinking degree	649:669	a crosslinking degree of around 90%	649:683	The obtained hydrogel, with a crosslinking degree of around 90%, showed interesting swelling properties.
25129803	1	42	theme	well-defined	117:128	arg1	hydrogel					130:137	a well-defined hydrogel	115:137	a well-defined hydrogel	115:137	In this work, a well-defined hydrogel was developed by coupling chitosan with PEO through "click chemistry".
25129803	4	43	theme	groups	562:567	arg1	amount					546:551	the amount	542:551	the amount	542:551	In fact, the extent of coupling is strictly dependent on the amount of azido groups on chitosan, which in turn can be easily modulated.
25129803	4	43	theme	groups	562:567	arg1	groups					562:567	azido groups	556:567	azido groups on chitosan	556:579	In fact, the extent of coupling is strictly dependent on the amount of azido groups on chitosan, which in turn can be easily modulated.
25129803	4	44	from	fact	488:491	arg1	dependent					529:537	dependent	529:537	dependent	529:537	In fact, the extent of coupling is strictly dependent on the amount of azido groups on chitosan, which in turn can be easily modulated.
25129803	4	44	from	fact	488:491	arg1	extent					498:503	the extent	494:503	the extent of coupling	494:515	In fact, the extent of coupling is strictly dependent on the amount of azido groups on chitosan, which in turn can be easily modulated.
25129803	4	45	theme	azido	556:560	arg1	groups					562:567	azido groups	556:567	azido groups on chitosan	556:579	In fact, the extent of coupling is strictly dependent on the amount of azido groups on chitosan, which in turn can be easily modulated.
27854436	1	0	theme	experimental	294:305	arg1	study					307:311	this experimental study	289:311	this experimental study	289:311	Characteristics of the buccal mucoadhesive films (film thickness, film weight, uniformity of mass and moisture content) prepared by solvent casting method were tested in this experimental study.
27854436	4	1	theme	carboxymethylcellulose	855:876	arg1	content					884:890	buccal mucoadhesive films solvent casting method sodium hyaluronate sodium carboxymethylcellulose water content	780:890	other tested parameters.Key words: buccal mucoadhesive films solvent casting method sodium hyaluronate sodium carboxymethylcellulose water content	745:890	The composition of the films influences also other tested parameters.Key words: buccal mucoadhesive films solvent casting method sodium hyaluronate sodium carboxymethylcellulose water content.
27854436	3	2	theme	polymers	671:678	arg1	ratio					649:653	the ratio	645:653	the ratio of mucoadhesive polymers in the composition	645:697	On the basis of the aforementioned tests, it was determined that water content was influenced by the molecular weight of sodium hyaluronate as well as by the ratio of mucoadhesive polymers in the composition.
27854436	0	3	from	influence	4:12	arg1	uniformity					59:68	uniformity	59:68	uniformity of mass	59:76	The influence of hyaluronan addition on thickness, weight, uniformity of mass and water content of mucoadhesive films.
27854436	0	3	from	influence	4:12	arg1	weight					51:56	weight	51:56	weight	51:56	The influence of hyaluronan addition on thickness, weight, uniformity of mass and water content of mucoadhesive films.
27854436	0	3	from	influence	4:12	arg1	thickness					40:48	thickness	40:48	thickness	40:48	The influence of hyaluronan addition on thickness, weight, uniformity of mass and water content of mucoadhesive films.
27854436	0	3	from	influence	4:12	arg1	content					88:94	water content	82:94	water content of mucoadhesive films	82:116	The influence of hyaluronan addition on thickness, weight, uniformity of mass and water content of mucoadhesive films.
27854436	4	4	theme	sodium	848:853	arg1	content					884:890	buccal mucoadhesive films solvent casting method sodium hyaluronate sodium carboxymethylcellulose water content	780:890	other tested parameters.Key words: buccal mucoadhesive films solvent casting method sodium hyaluronate sodium carboxymethylcellulose water content	745:890	The composition of the films influences also other tested parameters.Key words: buccal mucoadhesive films solvent casting method sodium hyaluronate sodium carboxymethylcellulose water content.
27854436	1	5	theme	buccal	142:147	arg1	uniformity					198:207	uniformity	198:207	uniformity of mass	198:215	Characteristics of the buccal mucoadhesive films (film thickness, film weight, uniformity of mass and moisture content) prepared by solvent casting method were tested in this experimental study.
27854436	1	5	theme	buccal	142:147	arg1	content					230:236	moisture content	221:236	moisture content	221:236	Characteristics of the buccal mucoadhesive films (film thickness, film weight, uniformity of mass and moisture content) prepared by solvent casting method were tested in this experimental study.
27854436	1	5	theme	buccal	142:147	arg1	films					162:166	the buccal mucoadhesive films	138:166	the buccal mucoadhesive films (film thickness, film weight, uniformity of mass and moisture content) prepared by solvent casting method	138:272	Characteristics of the buccal mucoadhesive films (film thickness, film weight, uniformity of mass and moisture content) prepared by solvent casting method were tested in this experimental study.
27854436	1	5	theme	buccal	142:147	arg1	thickness					174:182	film thickness	169:182	film thickness	169:182	Characteristics of the buccal mucoadhesive films (film thickness, film weight, uniformity of mass and moisture content) prepared by solvent casting method were tested in this experimental study.
27854436	1	5	theme	buccal	142:147	arg1	weight					190:195	film weight	185:195	film weight	185:195	Characteristics of the buccal mucoadhesive films (film thickness, film weight, uniformity of mass and moisture content) prepared by solvent casting method were tested in this experimental study.
27854436	1	6	theme	moisture	221:228	arg1	content					230:236	moisture content	221:236	moisture content	221:236	Characteristics of the buccal mucoadhesive films (film thickness, film weight, uniformity of mass and moisture content) prepared by solvent casting method were tested in this experimental study.
27854436	1	6	theme	moisture	221:228	arg1	films					162:166	the buccal mucoadhesive films	138:166	the buccal mucoadhesive films (film thickness, film weight, uniformity of mass and moisture content) prepared by solvent casting method	138:272	Characteristics of the buccal mucoadhesive films (film thickness, film weight, uniformity of mass and moisture content) prepared by solvent casting method were tested in this experimental study.
27854436	4	7	theme	solvent	806:812	arg1	content					884:890	buccal mucoadhesive films solvent casting method sodium hyaluronate sodium carboxymethylcellulose water content	780:890	other tested parameters.Key words: buccal mucoadhesive films solvent casting method sodium hyaluronate sodium carboxymethylcellulose water content	745:890	The composition of the films influences also other tested parameters.Key words: buccal mucoadhesive films solvent casting method sodium hyaluronate sodium carboxymethylcellulose water content.
27854436	0	8	theme	mass	73:76	arg1	uniformity					59:68	uniformity	59:68	uniformity of mass	59:76	The influence of hyaluronan addition on thickness, weight, uniformity of mass and water content of mucoadhesive films.
27854436	0	8	theme	mass	73:76	arg1	weight					51:56	weight	51:56	weight	51:56	The influence of hyaluronan addition on thickness, weight, uniformity of mass and water content of mucoadhesive films.
27854436	0	8	theme	mass	73:76	arg1	thickness					40:48	thickness	40:48	thickness	40:48	The influence of hyaluronan addition on thickness, weight, uniformity of mass and water content of mucoadhesive films.
27854436	0	8	theme	mass	73:76	arg1	content					88:94	water content	82:94	water content of mucoadhesive films	82:116	The influence of hyaluronan addition on thickness, weight, uniformity of mass and water content of mucoadhesive films.
27854436	4	9	theme	water	878:882	arg1	content					884:890	buccal mucoadhesive films solvent casting method sodium hyaluronate sodium carboxymethylcellulose water content	780:890	other tested parameters.Key words: buccal mucoadhesive films solvent casting method sodium hyaluronate sodium carboxymethylcellulose water content	745:890	The composition of the films influences also other tested parameters.Key words: buccal mucoadhesive films solvent casting method sodium hyaluronate sodium carboxymethylcellulose water content.
27854436	4	10	theme	films	723:727	arg1	composition					704:714	The composition	700:714	The composition of the films	700:727	The composition of the films influences also other tested parameters.Key words: buccal mucoadhesive films solvent casting method sodium hyaluronate sodium carboxymethylcellulose water content.
27854436	4	11	theme	method	822:827	arg1	content					884:890	buccal mucoadhesive films solvent casting method sodium hyaluronate sodium carboxymethylcellulose water content	780:890	other tested parameters.Key words: buccal mucoadhesive films solvent casting method sodium hyaluronate sodium carboxymethylcellulose water content	745:890	The composition of the films influences also other tested parameters.Key words: buccal mucoadhesive films solvent casting method sodium hyaluronate sodium carboxymethylcellulose water content.
27854436	4	12	theme	other	745:749	arg1	words					773:777	other tested parameters.Key words	745:777	other tested parameters.Key words: buccal mucoadhesive films solvent casting method sodium hyaluronate sodium carboxymethylcellulose water content	745:890	The composition of the films influences also other tested parameters.Key words: buccal mucoadhesive films solvent casting method sodium hyaluronate sodium carboxymethylcellulose water content.
27854436	1	13	theme	mucoadhesive	149:160	arg1	uniformity					198:207	uniformity	198:207	uniformity of mass	198:215	Characteristics of the buccal mucoadhesive films (film thickness, film weight, uniformity of mass and moisture content) prepared by solvent casting method were tested in this experimental study.
27854436	1	13	theme	mucoadhesive	149:160	arg1	content					230:236	moisture content	221:236	moisture content	221:236	Characteristics of the buccal mucoadhesive films (film thickness, film weight, uniformity of mass and moisture content) prepared by solvent casting method were tested in this experimental study.
27854436	1	13	theme	mucoadhesive	149:160	arg1	films					162:166	the buccal mucoadhesive films	138:166	the buccal mucoadhesive films (film thickness, film weight, uniformity of mass and moisture content) prepared by solvent casting method	138:272	Characteristics of the buccal mucoadhesive films (film thickness, film weight, uniformity of mass and moisture content) prepared by solvent casting method were tested in this experimental study.
27854436	1	13	theme	mucoadhesive	149:160	arg1	thickness					174:182	film thickness	169:182	film thickness	169:182	Characteristics of the buccal mucoadhesive films (film thickness, film weight, uniformity of mass and moisture content) prepared by solvent casting method were tested in this experimental study.
27854436	1	13	theme	mucoadhesive	149:160	arg1	weight					190:195	film weight	185:195	film weight	185:195	Characteristics of the buccal mucoadhesive films (film thickness, film weight, uniformity of mass and moisture content) prepared by solvent casting method were tested in this experimental study.
27854436	0	14	theme	water	82:86	arg1	content					88:94	water content	82:94	water content of mucoadhesive films	82:116	The influence of hyaluronan addition on thickness, weight, uniformity of mass and water content of mucoadhesive films.
27854436	4	15	theme	casting	814:820	arg1	content					884:890	buccal mucoadhesive films solvent casting method sodium hyaluronate sodium carboxymethylcellulose water content	780:890	other tested parameters.Key words: buccal mucoadhesive films solvent casting method sodium hyaluronate sodium carboxymethylcellulose water content	745:890	The composition of the films influences also other tested parameters.Key words: buccal mucoadhesive films solvent casting method sodium hyaluronate sodium carboxymethylcellulose water content.
27854436	3	16	theme	aforementioned	511:524	arg1	tests					526:530	the aforementioned tests	507:530	the aforementioned tests	507:530	On the basis of the aforementioned tests, it was determined that water content was influenced by the molecular weight of sodium hyaluronate as well as by the ratio of mucoadhesive polymers in the composition.
27854436	1	17	theme	films	162:166	arg1	Characteristics					119:133	Characteristics	119:133	Characteristics of the buccal mucoadhesive films (film thickness, film weight, uniformity of mass and moisture content) prepared by solvent casting method	119:272	Characteristics of the buccal mucoadhesive films (film thickness, film weight, uniformity of mass and moisture content) prepared by solvent casting method were tested in this experimental study.
27854436	2	18	theme	weights	423:429	arg1	hyaluronate					384:394	sodium hyaluronate	377:394	sodium hyaluronate of two different molecular weights and sodium carboxymethylcellulose	377:463	The formulations consisted either of one mucoadhesive polymer (sodium hyaluronate of two different molecular weights and sodium carboxymethylcellulose) or combinations thereof.
27854436	4	19	theme	hyaluronate	836:846	arg1	content					884:890	buccal mucoadhesive films solvent casting method sodium hyaluronate sodium carboxymethylcellulose water content	780:890	other tested parameters.Key words: buccal mucoadhesive films solvent casting method sodium hyaluronate sodium carboxymethylcellulose water content	745:890	The composition of the films influences also other tested parameters.Key words: buccal mucoadhesive films solvent casting method sodium hyaluronate sodium carboxymethylcellulose water content.
27854436	2	20	theme	molecular	413:421	arg1	weights					423:429	two different molecular weights	399:429	two different molecular weights	399:429	The formulations consisted either of one mucoadhesive polymer (sodium hyaluronate of two different molecular weights and sodium carboxymethylcellulose) or combinations thereof.
27854436	4	21	theme	sodium	829:834	arg1	content					884:890	buccal mucoadhesive films solvent casting method sodium hyaluronate sodium carboxymethylcellulose water content	780:890	other tested parameters.Key words: buccal mucoadhesive films solvent casting method sodium hyaluronate sodium carboxymethylcellulose water content	745:890	The composition of the films influences also other tested parameters.Key words: buccal mucoadhesive films solvent casting method sodium hyaluronate sodium carboxymethylcellulose water content.
27854436	1	22	theme	film	169:172	arg1	films					162:166	the buccal mucoadhesive films	138:166	the buccal mucoadhesive films (film thickness, film weight, uniformity of mass and moisture content) prepared by solvent casting method	138:272	Characteristics of the buccal mucoadhesive films (film thickness, film weight, uniformity of mass and moisture content) prepared by solvent casting method were tested in this experimental study.
27854436	1	22	theme	film	169:172	arg1	thickness					174:182	film thickness	169:182	film thickness	169:182	Characteristics of the buccal mucoadhesive films (film thickness, film weight, uniformity of mass and moisture content) prepared by solvent casting method were tested in this experimental study.
27854436	0	23	theme	hyaluronan	17:26	arg1	addition					28:35	hyaluronan addition	17:35	hyaluronan addition	17:35	The influence of hyaluronan addition on thickness, weight, uniformity of mass and water content of mucoadhesive films.
27854436	0	24	theme	mucoadhesive	99:110	arg1	films					112:116	mucoadhesive films	99:116	mucoadhesive films	99:116	The influence of hyaluronan addition on thickness, weight, uniformity of mass and water content of mucoadhesive films.
27854436	4	25	theme	films	800:804	arg1	content					884:890	buccal mucoadhesive films solvent casting method sodium hyaluronate sodium carboxymethylcellulose water content	780:890	other tested parameters.Key words: buccal mucoadhesive films solvent casting method sodium hyaluronate sodium carboxymethylcellulose water content	745:890	The composition of the films influences also other tested parameters.Key words: buccal mucoadhesive films solvent casting method sodium hyaluronate sodium carboxymethylcellulose water content.
27854436	1	26	theme	solvent	251:257	arg1	method					267:272	solvent casting method	251:272	solvent casting method	251:272	Characteristics of the buccal mucoadhesive films (film thickness, film weight, uniformity of mass and moisture content) prepared by solvent casting method were tested in this experimental study.
27854436	3	27	theme	hyaluronate	619:629	arg1	weight					602:607	the molecular weight	588:607	the molecular weight of sodium hyaluronate	588:629	On the basis of the aforementioned tests, it was determined that water content was influenced by the molecular weight of sodium hyaluronate as well as by the ratio of mucoadhesive polymers in the composition.
27854436	2	28	theme	carboxymethylcellulose	442:463	arg1	hyaluronate					384:394	sodium hyaluronate	377:394	sodium hyaluronate of two different molecular weights and sodium carboxymethylcellulose	377:463	The formulations consisted either of one mucoadhesive polymer (sodium hyaluronate of two different molecular weights and sodium carboxymethylcellulose) or combinations thereof.
27854436	3	29	from	ratio	649:653	arg1	composition					687:697	the composition	683:697	the composition	683:697	On the basis of the aforementioned tests, it was determined that water content was influenced by the molecular weight of sodium hyaluronate as well as by the ratio of mucoadhesive polymers in the composition.
27854436	1	30	theme	casting	259:265	arg1	method					267:272	solvent casting method	251:272	solvent casting method	251:272	Characteristics of the buccal mucoadhesive films (film thickness, film weight, uniformity of mass and moisture content) prepared by solvent casting method were tested in this experimental study.
27854436	1	31	theme	film	185:188	arg1	films					162:166	the buccal mucoadhesive films	138:166	the buccal mucoadhesive films (film thickness, film weight, uniformity of mass and moisture content) prepared by solvent casting method	138:272	Characteristics of the buccal mucoadhesive films (film thickness, film weight, uniformity of mass and moisture content) prepared by solvent casting method were tested in this experimental study.
27854436	1	31	theme	film	185:188	arg1	weight					190:195	film weight	185:195	film weight	185:195	Characteristics of the buccal mucoadhesive films (film thickness, film weight, uniformity of mass and moisture content) prepared by solvent casting method were tested in this experimental study.
27854436	4	32	theme	mucoadhesive	787:798	arg1	content					884:890	buccal mucoadhesive films solvent casting method sodium hyaluronate sodium carboxymethylcellulose water content	780:890	other tested parameters.Key words: buccal mucoadhesive films solvent casting method sodium hyaluronate sodium carboxymethylcellulose water content	745:890	The composition of the films influences also other tested parameters.Key words: buccal mucoadhesive films solvent casting method sodium hyaluronate sodium carboxymethylcellulose water content.
27854436	2	33	theme	sodium	377:382	arg1	hyaluronate					384:394	sodium hyaluronate	377:394	sodium hyaluronate of two different molecular weights and sodium carboxymethylcellulose	377:463	The formulations consisted either of one mucoadhesive polymer (sodium hyaluronate of two different molecular weights and sodium carboxymethylcellulose) or combinations thereof.
27854436	0	34	theme	addition	28:35	arg1	influence					4:12	The influence	0:12	The influence of hyaluronan addition on thickness, weight, uniformity of mass and water content of mucoadhesive films.	0:117	The influence of hyaluronan addition on thickness, weight, uniformity of mass and water content of mucoadhesive films.
27854436	0	35	theme	films	112:116	arg1	uniformity					59:68	uniformity	59:68	uniformity of mass	59:76	The influence of hyaluronan addition on thickness, weight, uniformity of mass and water content of mucoadhesive films.
27854436	0	35	theme	films	112:116	arg1	weight					51:56	weight	51:56	weight	51:56	The influence of hyaluronan addition on thickness, weight, uniformity of mass and water content of mucoadhesive films.
27854436	0	35	theme	films	112:116	arg1	thickness					40:48	thickness	40:48	thickness	40:48	The influence of hyaluronan addition on thickness, weight, uniformity of mass and water content of mucoadhesive films.
27854436	0	35	theme	films	112:116	arg1	content					88:94	water content	82:94	water content of mucoadhesive films	82:116	The influence of hyaluronan addition on thickness, weight, uniformity of mass and water content of mucoadhesive films.
27854436	2	36	theme	different	403:411	arg1	weights					423:429	two different molecular weights	399:429	two different molecular weights	399:429	The formulations consisted either of one mucoadhesive polymer (sodium hyaluronate of two different molecular weights and sodium carboxymethylcellulose) or combinations thereof.
27854436	2	37	theme	sodium	435:440	arg1	carboxymethylcellulose					442:463	sodium carboxymethylcellulose	435:463	sodium carboxymethylcellulose	435:463	The formulations consisted either of one mucoadhesive polymer (sodium hyaluronate of two different molecular weights and sodium carboxymethylcellulose) or combinations thereof.
27854436	3	38	theme	sodium	612:617	arg1	hyaluronate					619:629	sodium hyaluronate	612:629	sodium hyaluronate	612:629	On the basis of the aforementioned tests, it was determined that water content was influenced by the molecular weight of sodium hyaluronate as well as by the ratio of mucoadhesive polymers in the composition.
27854436	3	39	theme	water	556:560	arg1	content					562:568	water content	556:568	water content	556:568	On the basis of the aforementioned tests, it was determined that water content was influenced by the molecular weight of sodium hyaluronate as well as by the ratio of mucoadhesive polymers in the composition.
27854436	1	40	dep	films	162:166	arg1	films					162:166	the buccal mucoadhesive films	138:166	the buccal mucoadhesive films (film thickness, film weight, uniformity of mass and moisture content) prepared by solvent casting method	138:272	Characteristics of the buccal mucoadhesive films (film thickness, film weight, uniformity of mass and moisture content) prepared by solvent casting method were tested in this experimental study.
27854436	1	40	dep	films	162:166	arg1	content					230:236	moisture content	221:236	moisture content	221:236	Characteristics of the buccal mucoadhesive films (film thickness, film weight, uniformity of mass and moisture content) prepared by solvent casting method were tested in this experimental study.
27854436	1	40	dep	films	162:166	arg1	uniformity					198:207	uniformity	198:207	uniformity of mass	198:215	Characteristics of the buccal mucoadhesive films (film thickness, film weight, uniformity of mass and moisture content) prepared by solvent casting method were tested in this experimental study.
27854436	1	40	dep	films	162:166	arg1	thickness					174:182	film thickness	169:182	film thickness	169:182	Characteristics of the buccal mucoadhesive films (film thickness, film weight, uniformity of mass and moisture content) prepared by solvent casting method were tested in this experimental study.
27854436	1	40	dep	films	162:166	arg1	weight					190:195	film weight	185:195	film weight	185:195	Characteristics of the buccal mucoadhesive films (film thickness, film weight, uniformity of mass and moisture content) prepared by solvent casting method were tested in this experimental study.
27854436	4	41	theme	parameters.Key	758:771	arg1	words					773:777	other tested parameters.Key words	745:777	other tested parameters.Key words: buccal mucoadhesive films solvent casting method sodium hyaluronate sodium carboxymethylcellulose water content	745:890	The composition of the films influences also other tested parameters.Key words: buccal mucoadhesive films solvent casting method sodium hyaluronate sodium carboxymethylcellulose water content.
27854436	3	42	theme	molecular	592:600	arg1	weight					602:607	the molecular weight	588:607	the molecular weight of sodium hyaluronate	588:629	On the basis of the aforementioned tests, it was determined that water content was influenced by the molecular weight of sodium hyaluronate as well as by the ratio of mucoadhesive polymers in the composition.
27854436	4	43	theme	buccal	780:785	arg1	content					884:890	buccal mucoadhesive films solvent casting method sodium hyaluronate sodium carboxymethylcellulose water content	780:890	other tested parameters.Key words: buccal mucoadhesive films solvent casting method sodium hyaluronate sodium carboxymethylcellulose water content	745:890	The composition of the films influences also other tested parameters.Key words: buccal mucoadhesive films solvent casting method sodium hyaluronate sodium carboxymethylcellulose water content.
27854436	3	44	dep	tests	526:530	arg1	the					494:496	the	494:496	the	494:496	On the basis of the aforementioned tests, it was determined that water content was influenced by the molecular weight of sodium hyaluronate as well as by the ratio of mucoadhesive polymers in the composition.
27854436	3	44	dep	tests	526:530	arg1	basis					498:502	basis	498:502	basis	498:502	On the basis of the aforementioned tests, it was determined that water content was influenced by the molecular weight of sodium hyaluronate as well as by the ratio of mucoadhesive polymers in the composition.
27854436	4	45	theme	tested	751:756	arg1	words					773:777	other tested parameters.Key words	745:777	other tested parameters.Key words: buccal mucoadhesive films solvent casting method sodium hyaluronate sodium carboxymethylcellulose water content	745:890	The composition of the films influences also other tested parameters.Key words: buccal mucoadhesive films solvent casting method sodium hyaluronate sodium carboxymethylcellulose water content.
27854436	2	46	dep	polymer	368:374	arg1	hyaluronate					384:394	sodium hyaluronate	377:394	sodium hyaluronate of two different molecular weights and sodium carboxymethylcellulose	377:463	The formulations consisted either of one mucoadhesive polymer (sodium hyaluronate of two different molecular weights and sodium carboxymethylcellulose) or combinations thereof.
27854436	4	47	dep	words	773:777	arg1	content					884:890	buccal mucoadhesive films solvent casting method sodium hyaluronate sodium carboxymethylcellulose water content	780:890	other tested parameters.Key words: buccal mucoadhesive films solvent casting method sodium hyaluronate sodium carboxymethylcellulose water content	745:890	The composition of the films influences also other tested parameters.Key words: buccal mucoadhesive films solvent casting method sodium hyaluronate sodium carboxymethylcellulose water content.
27854436	2	48	theme	mucoadhesive	355:366	arg1	polymer					368:374	one mucoadhesive polymer	351:374	one mucoadhesive polymer (sodium hyaluronate of two different molecular weights and sodium carboxymethylcellulose)	351:464	The formulations consisted either of one mucoadhesive polymer (sodium hyaluronate of two different molecular weights and sodium carboxymethylcellulose) or combinations thereof.
27854436	3	49	theme	mucoadhesive	658:669	arg1	polymers					671:678	mucoadhesive polymers	658:678	mucoadhesive polymers	658:678	On the basis of the aforementioned tests, it was determined that water content was influenced by the molecular weight of sodium hyaluronate as well as by the ratio of mucoadhesive polymers in the composition.
27854436	1	50	theme	mass	212:215	arg1	films					162:166	the buccal mucoadhesive films	138:166	the buccal mucoadhesive films (film thickness, film weight, uniformity of mass and moisture content) prepared by solvent casting method	138:272	Characteristics of the buccal mucoadhesive films (film thickness, film weight, uniformity of mass and moisture content) prepared by solvent casting method were tested in this experimental study.
27854436	1	50	theme	mass	212:215	arg1	content					230:236	moisture content	221:236	moisture content	221:236	Characteristics of the buccal mucoadhesive films (film thickness, film weight, uniformity of mass and moisture content) prepared by solvent casting method were tested in this experimental study.
27854436	1	50	theme	mass	212:215	arg1	uniformity					198:207	uniformity	198:207	uniformity of mass	198:215	Characteristics of the buccal mucoadhesive films (film thickness, film weight, uniformity of mass and moisture content) prepared by solvent casting method were tested in this experimental study.
27854436	1	50	theme	mass	212:215	arg1	thickness					174:182	film thickness	169:182	film thickness	169:182	Characteristics of the buccal mucoadhesive films (film thickness, film weight, uniformity of mass and moisture content) prepared by solvent casting method were tested in this experimental study.
27854436	1	50	theme	mass	212:215	arg1	weight					190:195	film weight	185:195	film weight	185:195	Characteristics of the buccal mucoadhesive films (film thickness, film weight, uniformity of mass and moisture content) prepared by solvent casting method were tested in this experimental study.
26478423	5	0	theme	chitosan	985:992	arg1	hydrogels					994:1002	the chitosan hydrogels	981:1002	the chitosan hydrogels	981:1002	In addition, scanning electron microscopy (SEM) analysis indicated that the chitosan hydrogels exhibited 3D-porous structures, and the incorporation of collagen in the system caused increase on the average pore size.
26478423	1	1	dep	bone	286:289	arg1	repair					305:310	repair	305:310	repair	305:310	Recently, stimuli-responsive nanocomposite-derived hydrogels have gained prominence in tissue engineering because they can be applied as injectable scaffolds in bone and cartilage repair.
26478423	12	2	theme	embryo	2039:2044	arg1	effect					1937:1942	no toxic effect	1928:1942	no toxic effect of the composites on the human osteosarcoma cell culture (SAOS) and kidney cells line of human embryo (HEK 293 T)	1928:2056	The cytotoxicity and cell viability of the hydrogels were assessed by MTT and LIVE/DEAD® assays, where the results demonstrated no toxic effect of the composites on the human osteosarcoma cell culture (SAOS) and kidney cells line of human embryo (HEK 293 T).
26478423	6	3	theme	interactions	1280:1291	arg1	component					1227:1235	each component	1222:1235	each component of the composite system and their chemical interactions forming the scaffold	1222:1312	Fourier transform infrared spectroscopy (FTIR) analysis indicated the main functional groups of each component of the composite system and their chemical interactions forming the scaffold.
26478423	12	4	theme	HEK	2047:2049	arg1	T					2055:2055	HEK 293 T	2047:2055	HEK 293 T	2047:2055	The cytotoxicity and cell viability of the hydrogels were assessed by MTT and LIVE/DEAD® assays, where the results demonstrated no toxic effect of the composites on the human osteosarcoma cell culture (SAOS) and kidney cells line of human embryo (HEK 293 T).
26478423	12	4	theme	HEK	2047:2049	arg1	embryo					2039:2044	human embryo	2033:2044	human embryo (HEK 293 T)	2033:2056	The cytotoxicity and cell viability of the hydrogels were assessed by MTT and LIVE/DEAD® assays, where the results demonstrated no toxic effect of the composites on the human osteosarcoma cell culture (SAOS) and kidney cells line of human embryo (HEK 293 T).
26478423	12	5	theme	cells	2019:2023	arg1	line					2025:2028	the human osteosarcoma cell culture (SAOS) and kidney cells line	1965:2028	line	2025:2028	The cytotoxicity and cell viability of the hydrogels were assessed by MTT and LIVE/DEAD® assays, where the results demonstrated no toxic effect of the composites on the human osteosarcoma cell culture (SAOS) and kidney cells line of human embryo (HEK 293 T).
26478423	6	6	theme	system	1254:1259	arg1	component					1227:1235	each component	1222:1235	each component of the composite system and their chemical interactions forming the scaffold	1222:1312	Fourier transform infrared spectroscopy (FTIR) analysis indicated the main functional groups of each component of the composite system and their chemical interactions forming the scaffold.
26478423	10	7	theme	pure	1698:1701	arg1	chitosan					1703:1710	chitosan	1703:1710	chitosan	1703:1710	% of BG nanoparticles caused an increase of approximately 39% on stiffness compared to pure chitosan and the addition of 30 wt.
26478423	12	8	from	effect	1937:1942	arg1	line					2025:2028	the human osteosarcoma cell culture (SAOS) and kidney cells line	1965:2028	line	2025:2028	The cytotoxicity and cell viability of the hydrogels were assessed by MTT and LIVE/DEAD® assays, where the results demonstrated no toxic effect of the composites on the human osteosarcoma cell culture (SAOS) and kidney cells line of human embryo (HEK 293 T).
26478423	12	8	from	effect	1937:1942	arg1	SAOS					2002:2005	the human osteosarcoma cell culture (SAOS) and kidney cells line	1965:2028	SAOS	2002:2005	The cytotoxicity and cell viability of the hydrogels were assessed by MTT and LIVE/DEAD® assays, where the results demonstrated no toxic effect of the composites on the human osteosarcoma cell culture (SAOS) and kidney cells line of human embryo (HEK 293 T).
26478423	5	9	theme	electron	931:938	arg1	microscopy					940:949	scanning electron microscopy	922:949	scanning electron microscopy (SEM) analysis	922:964	In addition, scanning electron microscopy (SEM) analysis indicated that the chitosan hydrogels exhibited 3D-porous structures, and the incorporation of collagen in the system caused increase on the average pore size.
26478423	5	9	theme	electron	931:938	arg1	SEM					952:954	SEM	952:954	SEM	952:954	In addition, scanning electron microscopy (SEM) analysis indicated that the chitosan hydrogels exhibited 3D-porous structures, and the incorporation of collagen in the system caused increase on the average pore size.
26478423	5	10	theme	3D-porous	1014:1022	arg1	structures					1024:1033	3D-porous structures	1014:1033	3D-porous structures	1014:1033	In addition, scanning electron microscopy (SEM) analysis indicated that the chitosan hydrogels exhibited 3D-porous structures, and the incorporation of collagen in the system caused increase on the average pore size.
26478423	8	11	theme	gelation	1572:1579	arg1	process					1581:1587	the gelation process	1568:1587	the gelation process	1568:1587	The results demonstrated that the addition of collagen and bioactive glass increases the mechanical properties after the gelation process.
26478423	4	12	theme	gelation	811:818	arg1	compatible					865:874	compatible	865:874	compatible	865:874	The composites showed thermosensitive response with the gelation temperature at approximately 37 °C, which is compatible with the human body temperature.
26478423	4	12	theme	gelation	811:818	arg1	temperature					820:830	the gelation temperature	807:830	the gelation temperature	807:830	The composites showed thermosensitive response with the gelation temperature at approximately 37 °C, which is compatible with the human body temperature.
26478423	9	13	theme	wt	1608:1609	arg1	addition					1594:1601	The addition	1590:1601	The addition of 2 wt	1590:1609	The addition of 2 wt.
26478423	1	14	link	nanocomposite-derived	154:174	arg1	hydrogels					176:184	stimuli-responsive nanocomposite-derived hydrogels	135:184	stimuli-responsive nanocomposite-derived hydrogels	135:184	Recently, stimuli-responsive nanocomposite-derived hydrogels have gained prominence in tissue engineering because they can be applied as injectable scaffolds in bone and cartilage repair.
26478423	4	15	theme	thermosensitive	777:791	arg1	response					793:800	thermosensitive response	777:800	thermosensitive response	777:800	The composites showed thermosensitive response with the gelation temperature at approximately 37 °C, which is compatible with the human body temperature.
26478423	0	16	theme	tissue	105:110	arg1	engineering					112:122	tissue engineering	105:122	tissue engineering	105:122	Thermogelling chitosan-collagen-bioactive glass nanoparticle hybrids as potential injectable systems for tissue engineering.
26478423	12	17	theme	human	1969:1973	arg1	SAOS					2002:2005	the human osteosarcoma cell culture (SAOS) and kidney cells line	1965:2028	SAOS	2002:2005	The cytotoxicity and cell viability of the hydrogels were assessed by MTT and LIVE/DEAD® assays, where the results demonstrated no toxic effect of the composites on the human osteosarcoma cell culture (SAOS) and kidney cells line of human embryo (HEK 293 T).
26478423	2	18	theme	regenerative	591:602	arg1	applications					613:624	regenerative medicine applications	591:624	regenerative medicine applications	591:624	Due to the great potential of these systems, this study aimed to synthesize and characterize novel thermosensitive chitosan-based composites, chemically modified with collagen and reinforced by bioactive glass nanoparticles (BG) on the development of injectable nanohybrids for regenerative medicine applications.
26478423	6	19	dep	transform	1134:1142	arg1	infrared					1144:1151	infrared	1144:1151	transform infrared spectroscopy (FTIR) analysis	1134:1180	Fourier transform infrared spectroscopy (FTIR) analysis indicated the main functional groups of each component of the composite system and their chemical interactions forming the scaffold.
26478423	12	20	theme	cell	1988:1991	arg1	SAOS					2002:2005	the human osteosarcoma cell culture (SAOS) and kidney cells line	1965:2028	SAOS	2002:2005	The cytotoxicity and cell viability of the hydrogels were assessed by MTT and LIVE/DEAD® assays, where the results demonstrated no toxic effect of the composites on the human osteosarcoma cell culture (SAOS) and kidney cells line of human embryo (HEK 293 T).
26478423	2	21	theme	nanohybrids	575:585	arg1	development					549:559	the development	545:559	the development of injectable nanohybrids for regenerative medicine applications	545:624	Due to the great potential of these systems, this study aimed to synthesize and characterize novel thermosensitive chitosan-based composites, chemically modified with collagen and reinforced by bioactive glass nanoparticles (BG) on the development of injectable nanohybrids for regenerative medicine applications.
26478423	2	22	from	nanoparticles	523:535	arg1	development					549:559	the development	545:559	the development of injectable nanohybrids for regenerative medicine applications	545:624	Due to the great potential of these systems, this study aimed to synthesize and characterize novel thermosensitive chitosan-based composites, chemically modified with collagen and reinforced by bioactive glass nanoparticles (BG) on the development of injectable nanohybrids for regenerative medicine applications.
26478423	7	23	theme	temperature	1438:1448	arg1	behavior					1391:1398	the viscoelastic behavior	1374:1398	the viscoelastic behavior of the hydrogels	1374:1415	Moreover, rheological measurements were employed to assess the viscoelastic behavior of the hydrogels as a function of the temperature.
26478423	7	23	theme	temperature	1438:1448	arg1	function					1422:1429	a function	1420:1429	a function of the temperature	1420:1448	Moreover, rheological measurements were employed to assess the viscoelastic behavior of the hydrogels as a function of the temperature.
26478423	2	24	theme	glass	517:521	arg1	BG					538:539	BG	538:539	BG	538:539	Due to the great potential of these systems, this study aimed to synthesize and characterize novel thermosensitive chitosan-based composites, chemically modified with collagen and reinforced by bioactive glass nanoparticles (BG) on the development of injectable nanohybrids for regenerative medicine applications.
26478423	2	24	theme	glass	517:521	arg1	nanoparticles					523:535	bioactive glass nanoparticles	507:535	bioactive glass nanoparticles (BG) on the development of injectable nanohybrids for regenerative medicine applications	507:624	Due to the great potential of these systems, this study aimed to synthesize and characterize novel thermosensitive chitosan-based composites, chemically modified with collagen and reinforced by bioactive glass nanoparticles (BG) on the development of injectable nanohybrids for regenerative medicine applications.
26478423	10	25	theme	wt	1735:1736	arg1	chitosan					1703:1710	chitosan	1703:1710	chitosan	1703:1710	% of BG nanoparticles caused an increase of approximately 39% on stiffness compared to pure chitosan and the addition of 30 wt.
26478423	10	25	theme	wt	1735:1736	arg1	addition					1720:1727	the addition	1716:1727	the addition of 30 wt	1716:1736	% of BG nanoparticles caused an increase of approximately 39% on stiffness compared to pure chitosan and the addition of 30 wt.
26478423	0	26	theme	glass	42:46	arg1	systems					93:99	potential injectable systems	72:99	potential injectable systems for tissue engineering	72:122	Thermogelling chitosan-collagen-bioactive glass nanoparticle hybrids as potential injectable systems for tissue engineering.
26478423	0	26	theme	glass	42:46	arg1	hybrids					61:67	chitosan-collagen-bioactive glass nanoparticle hybrids	14:67	chitosan-collagen-bioactive glass nanoparticle hybrids	14:67	Thermogelling chitosan-collagen-bioactive glass nanoparticle hybrids as potential injectable systems for tissue engineering.
26478423	1	27	theme	stimuli-responsive	135:152	arg1	hydrogels					176:184	stimuli-responsive nanocomposite-derived hydrogels	135:184	stimuli-responsive nanocomposite-derived hydrogels	135:184	Recently, stimuli-responsive nanocomposite-derived hydrogels have gained prominence in tissue engineering because they can be applied as injectable scaffolds in bone and cartilage repair.
26478423	3	28	theme	biological	735:744	arg1	testing					746:752	structural, morphological, rheological, and biological testing	691:752	structural, morphological, rheological, and biological testing	691:752	Thus, the composite hydrogels were extensively characterized by structural, morphological, rheological, and biological testing.
26478423	13	29	theme	thermoresponsive	2209:2224	arg1	biomaterials					2226:2237	thermoresponsive biomaterials	2209:2237	thermoresponsive biomaterials for bone-tissue bioapplications	2209:2269	Hence, it can be stated that innovative composites were successfully designed and synthesized in this research with promising potential to be used as thermoresponsive biomaterials for bone-tissue bioapplications.
26478423	5	30	theme	pore	1115:1118	arg1	size					1120:1123	the average pore size	1103:1123	the average pore size	1103:1123	In addition, scanning electron microscopy (SEM) analysis indicated that the chitosan hydrogels exhibited 3D-porous structures, and the incorporation of collagen in the system caused increase on the average pore size.
26478423	12	31	theme	LIVE/DEAD®	1878:1887	arg1	assays					1889:1894	MTT and LIVE/DEAD® assays	1870:1894	assays	1889:1894	The cytotoxicity and cell viability of the hydrogels were assessed by MTT and LIVE/DEAD® assays, where the results demonstrated no toxic effect of the composites on the human osteosarcoma cell culture (SAOS) and kidney cells line of human embryo (HEK 293 T).
26478423	7	32	theme	hydrogels	1407:1415	arg1	behavior					1391:1398	the viscoelastic behavior	1374:1398	the viscoelastic behavior of the hydrogels	1374:1415	Moreover, rheological measurements were employed to assess the viscoelastic behavior of the hydrogels as a function of the temperature.
26478423	7	32	theme	hydrogels	1407:1415	arg1	function					1422:1429	a function	1420:1429	a function of the temperature	1420:1448	Moreover, rheological measurements were employed to assess the viscoelastic behavior of the hydrogels as a function of the temperature.
26478423	8	33	theme	glass	1520:1524	arg1	addition					1485:1492	the addition	1481:1492	the addition of collagen and bioactive glass	1481:1524	The results demonstrated that the addition of collagen and bioactive glass increases the mechanical properties after the gelation process.
26478423	0	34	theme	potential	72:80	arg1	systems					93:99	potential injectable systems	72:99	potential injectable systems for tissue engineering	72:122	Thermogelling chitosan-collagen-bioactive glass nanoparticle hybrids as potential injectable systems for tissue engineering.
26478423	0	34	theme	potential	72:80	arg1	hybrids					61:67	chitosan-collagen-bioactive glass nanoparticle hybrids	14:67	chitosan-collagen-bioactive glass nanoparticle hybrids	14:67	Thermogelling chitosan-collagen-bioactive glass nanoparticle hybrids as potential injectable systems for tissue engineering.
26478423	10	35	theme	BG	1616:1617	arg1	nanoparticles					1619:1631	BG nanoparticles	1616:1631	BG nanoparticles	1616:1631	% of BG nanoparticles caused an increase of approximately 39% on stiffness compared to pure chitosan and the addition of 30 wt.
26478423	4	36	theme	human	885:889	arg1	temperature					896:906	the human body temperature	881:906	the human body temperature	881:906	The composites showed thermosensitive response with the gelation temperature at approximately 37 °C, which is compatible with the human body temperature.
26478423	7	37	theme	viscoelastic	1378:1389	arg1	behavior					1391:1398	the viscoelastic behavior	1374:1398	the viscoelastic behavior of the hydrogels	1374:1415	Moreover, rheological measurements were employed to assess the viscoelastic behavior of the hydrogels as a function of the temperature.
26478423	7	37	theme	viscoelastic	1378:1389	arg1	function					1422:1429	a function	1420:1429	a function of the temperature	1420:1448	Moreover, rheological measurements were employed to assess the viscoelastic behavior of the hydrogels as a function of the temperature.
26478423	2	38	theme	thermosensitive	412:426	arg1	composites					443:452	novel thermosensitive chitosan-based composites	406:452	novel thermosensitive chitosan-based composites	406:452	Due to the great potential of these systems, this study aimed to synthesize and characterize novel thermosensitive chitosan-based composites, chemically modified with collagen and reinforced by bioactive glass nanoparticles (BG) on the development of injectable nanohybrids for regenerative medicine applications.
26478423	12	39	theme	MTT	1870:1872	arg1	assays					1889:1894	MTT and LIVE/DEAD® assays	1870:1894	assays	1889:1894	The cytotoxicity and cell viability of the hydrogels were assessed by MTT and LIVE/DEAD® assays, where the results demonstrated no toxic effect of the composites on the human osteosarcoma cell culture (SAOS) and kidney cells line of human embryo (HEK 293 T).
26478423	6	40	theme	main	1196:1199	arg1	groups					1212:1217	the main functional groups	1192:1217	the main functional groups of each component of the composite system and their chemical interactions forming the scaffold	1192:1312	Fourier transform infrared spectroscopy (FTIR) analysis indicated the main functional groups of each component of the composite system and their chemical interactions forming the scaffold.
26478423	6	40	theme	main	1196:1199	arg1	component					1227:1235	each component	1222:1235	each component of the composite system and their chemical interactions forming the scaffold	1222:1312	Fourier transform infrared spectroscopy (FTIR) analysis indicated the main functional groups of each component of the composite system and their chemical interactions forming the scaffold.
26478423	11	41	theme	%	1738:1738	arg1	collagen					1740:1747	% collagen	1738:1747	% collagen	1738:1747	% collagen caused a further increase on the stiffness by 95%.
26478423	13	42	theme	promising	2175:2183	arg1	potential					2185:2193	promising potential	2175:2193	promising potential to be used as thermoresponsive biomaterials for bone-tissue bioapplications	2175:2269	Hence, it can be stated that innovative composites were successfully designed and synthesized in this research with promising potential to be used as thermoresponsive biomaterials for bone-tissue bioapplications.
26478423	12	43	theme	human	2033:2037	arg1	T					2055:2055	HEK 293 T	2047:2055	HEK 293 T	2047:2055	The cytotoxicity and cell viability of the hydrogels were assessed by MTT and LIVE/DEAD® assays, where the results demonstrated no toxic effect of the composites on the human osteosarcoma cell culture (SAOS) and kidney cells line of human embryo (HEK 293 T).
26478423	12	43	theme	human	2033:2037	arg1	embryo					2039:2044	human embryo	2033:2044	human embryo (HEK 293 T)	2033:2056	The cytotoxicity and cell viability of the hydrogels were assessed by MTT and LIVE/DEAD® assays, where the results demonstrated no toxic effect of the composites on the human osteosarcoma cell culture (SAOS) and kidney cells line of human embryo (HEK 293 T).
26478423	12	44	theme	hydrogels	1843:1851	arg1	cytotoxicity					1804:1815	cytotoxicity	1804:1815	cytotoxicity	1804:1815	The cytotoxicity and cell viability of the hydrogels were assessed by MTT and LIVE/DEAD® assays, where the results demonstrated no toxic effect of the composites on the human osteosarcoma cell culture (SAOS) and kidney cells line of human embryo (HEK 293 T).
26478423	12	44	theme	hydrogels	1843:1851	arg1	viability					1826:1834	cell viability	1821:1834	cell viability	1821:1834	The cytotoxicity and cell viability of the hydrogels were assessed by MTT and LIVE/DEAD® assays, where the results demonstrated no toxic effect of the composites on the human osteosarcoma cell culture (SAOS) and kidney cells line of human embryo (HEK 293 T).
26478423	6	45	theme	chemical	1271:1278	arg1	interactions					1280:1291	their chemical interactions	1265:1291	their chemical interactions forming the scaffold	1265:1312	Fourier transform infrared spectroscopy (FTIR) analysis indicated the main functional groups of each component of the composite system and their chemical interactions forming the scaffold.
26478423	10	46	theme	%	1671:1671	arg1	increase					1643:1650	an increase	1640:1650	an increase of approximately 39%	1640:1671	% of BG nanoparticles caused an increase of approximately 39% on stiffness compared to pure chitosan and the addition of 30 wt.
26478423	11	47	theme	further	1758:1764	arg1	increase					1766:1773	a further increase	1756:1773	a further increase on the stiffness by 95%	1756:1797	% collagen caused a further increase on the stiffness by 95%.
26478423	2	48	theme	great	324:328	arg1	potential					330:338	the great potential	320:338	the great potential of these systems	320:355	Due to the great potential of these systems, this study aimed to synthesize and characterize novel thermosensitive chitosan-based composites, chemically modified with collagen and reinforced by bioactive glass nanoparticles (BG) on the development of injectable nanohybrids for regenerative medicine applications.
26478423	3	49	theme	composite	637:645	arg1	hydrogels					647:655	the composite hydrogels	633:655	the composite hydrogels	633:655	Thus, the composite hydrogels were extensively characterized by structural, morphological, rheological, and biological testing.
26478423	5	50	from	increase	1091:1098	arg1	size					1120:1123	the average pore size	1103:1123	the average pore size	1103:1123	In addition, scanning electron microscopy (SEM) analysis indicated that the chitosan hydrogels exhibited 3D-porous structures, and the incorporation of collagen in the system caused increase on the average pore size.
26478423	12	51	theme	kidney	2012:2017	arg1	line					2025:2028	the human osteosarcoma cell culture (SAOS) and kidney cells line	1965:2028	line	2025:2028	The cytotoxicity and cell viability of the hydrogels were assessed by MTT and LIVE/DEAD® assays, where the results demonstrated no toxic effect of the composites on the human osteosarcoma cell culture (SAOS) and kidney cells line of human embryo (HEK 293 T).
26478423	12	52	theme	cell	1821:1824	arg1	viability					1826:1834	cell viability	1821:1834	cell viability	1821:1834	The cytotoxicity and cell viability of the hydrogels were assessed by MTT and LIVE/DEAD® assays, where the results demonstrated no toxic effect of the composites on the human osteosarcoma cell culture (SAOS) and kidney cells line of human embryo (HEK 293 T).
26478423	6	53	theme	composite	1244:1252	arg1	system					1254:1259	the composite system	1240:1259	the composite system	1240:1259	Fourier transform infrared spectroscopy (FTIR) analysis indicated the main functional groups of each component of the composite system and their chemical interactions forming the scaffold.
26478423	4	54	with	compatible	865:874	arg1	temperature					896:906	the human body temperature	881:906	the human body temperature	881:906	The composites showed thermosensitive response with the gelation temperature at approximately 37 °C, which is compatible with the human body temperature.
26478423	2	55	theme	systems	349:355	arg1	potential					330:338	the great potential	320:338	the great potential of these systems	320:355	Due to the great potential of these systems, this study aimed to synthesize and characterize novel thermosensitive chitosan-based composites, chemically modified with collagen and reinforced by bioactive glass nanoparticles (BG) on the development of injectable nanohybrids for regenerative medicine applications.
26478423	5	56	theme	scanning	922:929	arg1	microscopy					940:949	scanning electron microscopy	922:949	scanning electron microscopy (SEM) analysis	922:964	In addition, scanning electron microscopy (SEM) analysis indicated that the chitosan hydrogels exhibited 3D-porous structures, and the incorporation of collagen in the system caused increase on the average pore size.
26478423	5	56	theme	scanning	922:929	arg1	SEM					952:954	SEM	952:954	SEM	952:954	In addition, scanning electron microscopy (SEM) analysis indicated that the chitosan hydrogels exhibited 3D-porous structures, and the incorporation of collagen in the system caused increase on the average pore size.
26478423	5	57	theme	microscopy	940:949	arg1	analysis					957:964	scanning electron microscopy (SEM) analysis	922:964	scanning electron microscopy (SEM) analysis	922:964	In addition, scanning electron microscopy (SEM) analysis indicated that the chitosan hydrogels exhibited 3D-porous structures, and the incorporation of collagen in the system caused increase on the average pore size.
26478423	2	58	theme	medicine	604:611	arg1	applications					613:624	regenerative medicine applications	591:624	regenerative medicine applications	591:624	Due to the great potential of these systems, this study aimed to synthesize and characterize novel thermosensitive chitosan-based composites, chemically modified with collagen and reinforced by bioactive glass nanoparticles (BG) on the development of injectable nanohybrids for regenerative medicine applications.
26478423	13	59	theme	bone-tissue	2243:2253	arg1	bioapplications					2255:2269	bone-tissue bioapplications	2243:2269	bone-tissue bioapplications	2243:2269	Hence, it can be stated that innovative composites were successfully designed and synthesized in this research with promising potential to be used as thermoresponsive biomaterials for bone-tissue bioapplications.
26478423	11	60	from	increase	1766:1773	arg1	stiffness					1782:1790	the stiffness	1778:1790	the stiffness by 95%	1778:1797	% collagen caused a further increase on the stiffness by 95%.
26478423	1	61	from	scaffolds	273:281	arg1	cartilage					295:303	cartilage	295:303	cartilage	295:303	Recently, stimuli-responsive nanocomposite-derived hydrogels have gained prominence in tissue engineering because they can be applied as injectable scaffolds in bone and cartilage repair.
26478423	1	61	from	scaffolds	273:281	arg1	bone					286:289	bone	286:289	bone	286:289	Recently, stimuli-responsive nanocomposite-derived hydrogels have gained prominence in tissue engineering because they can be applied as injectable scaffolds in bone and cartilage repair.
26478423	1	62	theme	nanocomposite-derived	154:174	arg1	hydrogels					176:184	stimuli-responsive nanocomposite-derived hydrogels	135:184	stimuli-responsive nanocomposite-derived hydrogels	135:184	Recently, stimuli-responsive nanocomposite-derived hydrogels have gained prominence in tissue engineering because they can be applied as injectable scaffolds in bone and cartilage repair.
26478423	3	63	theme	morphological	703:715	arg1	testing					746:752	structural, morphological, rheological, and biological testing	691:752	structural, morphological, rheological, and biological testing	691:752	Thus, the composite hydrogels were extensively characterized by structural, morphological, rheological, and biological testing.
26478423	12	64	dep	cytotoxicity	1804:1815	arg1	The					1800:1802	The	1800:1802	The	1800:1802	The cytotoxicity and cell viability of the hydrogels were assessed by MTT and LIVE/DEAD® assays, where the results demonstrated no toxic effect of the composites on the human osteosarcoma cell culture (SAOS) and kidney cells line of human embryo (HEK 293 T).
26478423	7	65	theme	rheological	1325:1335	arg1	measurements					1337:1348	rheological measurements	1325:1348	rheological measurements	1325:1348	Moreover, rheological measurements were employed to assess the viscoelastic behavior of the hydrogels as a function of the temperature.
26478423	3	66	theme	structural	691:700	arg1	testing					746:752	structural, morphological, rheological, and biological testing	691:752	structural, morphological, rheological, and biological testing	691:752	Thus, the composite hydrogels were extensively characterized by structural, morphological, rheological, and biological testing.
26478423	12	67	theme	osteosarcoma	1975:1986	arg1	SAOS					2002:2005	the human osteosarcoma cell culture (SAOS) and kidney cells line	1965:2028	SAOS	2002:2005	The cytotoxicity and cell viability of the hydrogels were assessed by MTT and LIVE/DEAD® assays, where the results demonstrated no toxic effect of the composites on the human osteosarcoma cell culture (SAOS) and kidney cells line of human embryo (HEK 293 T).
26478423	1	68	theme	tissue	212:217	arg1	engineering					219:229	tissue engineering	212:229	tissue engineering	212:229	Recently, stimuli-responsive nanocomposite-derived hydrogels have gained prominence in tissue engineering because they can be applied as injectable scaffolds in bone and cartilage repair.
26478423	13	69	theme	innovative	2088:2097	arg1	composites					2099:2108	innovative composites	2088:2108	innovative composites	2088:2108	Hence, it can be stated that innovative composites were successfully designed and synthesized in this research with promising potential to be used as thermoresponsive biomaterials for bone-tissue bioapplications.
26478423	12	70	theme	culture	1993:1999	arg1	SAOS					2002:2005	the human osteosarcoma cell culture (SAOS) and kidney cells line	1965:2028	SAOS	2002:2005	The cytotoxicity and cell viability of the hydrogels were assessed by MTT and LIVE/DEAD® assays, where the results demonstrated no toxic effect of the composites on the human osteosarcoma cell culture (SAOS) and kidney cells line of human embryo (HEK 293 T).
26478423	5	71	theme	collagen	1061:1068	arg1	incorporation					1044:1056	the incorporation	1040:1056	the incorporation of collagen in the system	1040:1082	In addition, scanning electron microscopy (SEM) analysis indicated that the chitosan hydrogels exhibited 3D-porous structures, and the incorporation of collagen in the system caused increase on the average pore size.
26478423	6	72	dep	Fourier	1126:1132	arg1	transform					1134:1142	transform	1134:1142	transform infrared spectroscopy (FTIR) analysis	1134:1180	Fourier transform infrared spectroscopy (FTIR) analysis indicated the main functional groups of each component of the composite system and their chemical interactions forming the scaffold.
26478423	12	73	theme	composites	1951:1960	arg1	effect					1937:1942	no toxic effect	1928:1942	no toxic effect of the composites on the human osteosarcoma cell culture (SAOS) and kidney cells line of human embryo (HEK 293 T)	1928:2056	The cytotoxicity and cell viability of the hydrogels were assessed by MTT and LIVE/DEAD® assays, where the results demonstrated no toxic effect of the composites on the human osteosarcoma cell culture (SAOS) and kidney cells line of human embryo (HEK 293 T).
26478423	2	74	theme	injectable	564:573	arg1	nanohybrids					575:585	injectable nanohybrids	564:585	injectable nanohybrids for regenerative medicine applications	564:624	Due to the great potential of these systems, this study aimed to synthesize and characterize novel thermosensitive chitosan-based composites, chemically modified with collagen and reinforced by bioactive glass nanoparticles (BG) on the development of injectable nanohybrids for regenerative medicine applications.
26478423	0	75	theme	chitosan-collagen-bioactive	14:40	arg1	systems					93:99	potential injectable systems	72:99	potential injectable systems for tissue engineering	72:122	Thermogelling chitosan-collagen-bioactive glass nanoparticle hybrids as potential injectable systems for tissue engineering.
26478423	0	75	theme	chitosan-collagen-bioactive	14:40	arg1	hybrids					61:67	chitosan-collagen-bioactive glass nanoparticle hybrids	14:67	chitosan-collagen-bioactive glass nanoparticle hybrids	14:67	Thermogelling chitosan-collagen-bioactive glass nanoparticle hybrids as potential injectable systems for tissue engineering.
26478423	4	76	from	°C	852:853	arg1	compatible					865:874	compatible	865:874	compatible	865:874	The composites showed thermosensitive response with the gelation temperature at approximately 37 °C, which is compatible with the human body temperature.
26478423	4	76	from	°C	852:853	arg1	temperature					820:830	the gelation temperature	807:830	the gelation temperature	807:830	The composites showed thermosensitive response with the gelation temperature at approximately 37 °C, which is compatible with the human body temperature.
26478423	2	77	theme	bioactive	507:515	arg1	BG					538:539	BG	538:539	BG	538:539	Due to the great potential of these systems, this study aimed to synthesize and characterize novel thermosensitive chitosan-based composites, chemically modified with collagen and reinforced by bioactive glass nanoparticles (BG) on the development of injectable nanohybrids for regenerative medicine applications.
26478423	2	77	theme	bioactive	507:515	arg1	nanoparticles					523:535	bioactive glass nanoparticles	507:535	bioactive glass nanoparticles (BG) on the development of injectable nanohybrids for regenerative medicine applications	507:624	Due to the great potential of these systems, this study aimed to synthesize and characterize novel thermosensitive chitosan-based composites, chemically modified with collagen and reinforced by bioactive glass nanoparticles (BG) on the development of injectable nanohybrids for regenerative medicine applications.
26478423	2	78	theme	chitosan-based	428:441	arg1	composites					443:452	novel thermosensitive chitosan-based composites	406:452	novel thermosensitive chitosan-based composites	406:452	Due to the great potential of these systems, this study aimed to synthesize and characterize novel thermosensitive chitosan-based composites, chemically modified with collagen and reinforced by bioactive glass nanoparticles (BG) on the development of injectable nanohybrids for regenerative medicine applications.
26478423	0	79	theme	nanoparticle	48:59	arg1	systems					93:99	potential injectable systems	72:99	potential injectable systems for tissue engineering	72:122	Thermogelling chitosan-collagen-bioactive glass nanoparticle hybrids as potential injectable systems for tissue engineering.
26478423	0	79	theme	nanoparticle	48:59	arg1	hybrids					61:67	chitosan-collagen-bioactive glass nanoparticle hybrids	14:67	chitosan-collagen-bioactive glass nanoparticle hybrids	14:67	Thermogelling chitosan-collagen-bioactive glass nanoparticle hybrids as potential injectable systems for tissue engineering.
26478423	12	80	theme	toxic	1931:1935	arg1	effect					1937:1942	no toxic effect	1928:1942	no toxic effect of the composites on the human osteosarcoma cell culture (SAOS) and kidney cells line of human embryo (HEK 293 T)	1928:2056	The cytotoxicity and cell viability of the hydrogels were assessed by MTT and LIVE/DEAD® assays, where the results demonstrated no toxic effect of the composites on the human osteosarcoma cell culture (SAOS) and kidney cells line of human embryo (HEK 293 T).
26478423	6	81	theme	spectroscopy	1153:1164	arg1	analysis					1173:1180	spectroscopy (FTIR) analysis	1153:1180	spectroscopy (FTIR) analysis	1153:1180	Fourier transform infrared spectroscopy (FTIR) analysis indicated the main functional groups of each component of the composite system and their chemical interactions forming the scaffold.
26478423	1	82	theme	injectable	262:271	arg1	they					239:242	they	239:242	they	239:242	Recently, stimuli-responsive nanocomposite-derived hydrogels have gained prominence in tissue engineering because they can be applied as injectable scaffolds in bone and cartilage repair.
26478423	1	82	theme	injectable	262:271	arg1	scaffolds					273:281	injectable scaffolds	262:281	injectable scaffolds in bone and cartilage repair	262:310	Recently, stimuli-responsive nanocomposite-derived hydrogels have gained prominence in tissue engineering because they can be applied as injectable scaffolds in bone and cartilage repair.
26478423	5	83	theme	average	1107:1113	arg1	size					1120:1123	the average pore size	1103:1123	the average pore size	1103:1123	In addition, scanning electron microscopy (SEM) analysis indicated that the chitosan hydrogels exhibited 3D-porous structures, and the incorporation of collagen in the system caused increase on the average pore size.
26478423	3	84	theme	rheological	718:728	arg1	testing					746:752	structural, morphological, rheological, and biological testing	691:752	structural, morphological, rheological, and biological testing	691:752	Thus, the composite hydrogels were extensively characterized by structural, morphological, rheological, and biological testing.
26478423	8	85	theme	collagen	1497:1504	arg1	addition					1485:1492	the addition	1481:1492	the addition of collagen and bioactive glass	1481:1524	The results demonstrated that the addition of collagen and bioactive glass increases the mechanical properties after the gelation process.
26478423	0	86	theme	injectable	82:91	arg1	systems					93:99	potential injectable systems	72:99	potential injectable systems for tissue engineering	72:122	Thermogelling chitosan-collagen-bioactive glass nanoparticle hybrids as potential injectable systems for tissue engineering.
26478423	0	86	theme	injectable	82:91	arg1	hybrids					61:67	chitosan-collagen-bioactive glass nanoparticle hybrids	14:67	chitosan-collagen-bioactive glass nanoparticle hybrids	14:67	Thermogelling chitosan-collagen-bioactive glass nanoparticle hybrids as potential injectable systems for tissue engineering.
26478423	6	87	theme	component	1227:1235	arg1	groups					1212:1217	the main functional groups	1192:1217	the main functional groups of each component of the composite system and their chemical interactions forming the scaffold	1192:1312	Fourier transform infrared spectroscopy (FTIR) analysis indicated the main functional groups of each component of the composite system and their chemical interactions forming the scaffold.
26478423	6	87	theme	component	1227:1235	arg1	component					1227:1235	each component	1222:1235	each component of the composite system and their chemical interactions forming the scaffold	1222:1312	Fourier transform infrared spectroscopy (FTIR) analysis indicated the main functional groups of each component of the composite system and their chemical interactions forming the scaffold.
26478423	10	88	theme	nanoparticles	1619:1631	arg1	%					1611:1611	%	1611:1611	% of BG nanoparticles	1611:1631	% of BG nanoparticles caused an increase of approximately 39% on stiffness compared to pure chitosan and the addition of 30 wt.
26478423	10	88	theme	nanoparticles	1619:1631	arg1	nanoparticles					1619:1631	BG nanoparticles	1616:1631	BG nanoparticles	1616:1631	% of BG nanoparticles caused an increase of approximately 39% on stiffness compared to pure chitosan and the addition of 30 wt.
26478423	8	89	theme	bioactive	1510:1518	arg1	glass					1520:1524	bioactive glass	1510:1524	bioactive glass	1510:1524	The results demonstrated that the addition of collagen and bioactive glass increases the mechanical properties after the gelation process.
26478423	5	90	from	incorporation	1044:1056	arg1	system					1077:1082	the system	1073:1082	the system	1073:1082	In addition, scanning electron microscopy (SEM) analysis indicated that the chitosan hydrogels exhibited 3D-porous structures, and the incorporation of collagen in the system caused increase on the average pore size.
26478423	4	91	theme	body	891:894	arg1	temperature					896:906	the human body temperature	881:906	the human body temperature	881:906	The composites showed thermosensitive response with the gelation temperature at approximately 37 °C, which is compatible with the human body temperature.
26478423	6	92	theme	functional	1201:1210	arg1	groups					1212:1217	the main functional groups	1192:1217	the main functional groups of each component of the composite system and their chemical interactions forming the scaffold	1192:1312	Fourier transform infrared spectroscopy (FTIR) analysis indicated the main functional groups of each component of the composite system and their chemical interactions forming the scaffold.
26478423	6	92	theme	functional	1201:1210	arg1	component					1227:1235	each component	1222:1235	each component of the composite system and their chemical interactions forming the scaffold	1222:1312	Fourier transform infrared spectroscopy (FTIR) analysis indicated the main functional groups of each component of the composite system and their chemical interactions forming the scaffold.
26478423	8	93	theme	mechanical	1540:1549	arg1	properties					1551:1560	the mechanical properties	1536:1560	the mechanical properties after the gelation process	1536:1587	The results demonstrated that the addition of collagen and bioactive glass increases the mechanical properties after the gelation process.
26478423	2	94	theme	novel	406:410	arg1	composites					443:452	novel thermosensitive chitosan-based composites	406:452	novel thermosensitive chitosan-based composites	406:452	Due to the great potential of these systems, this study aimed to synthesize and characterize novel thermosensitive chitosan-based composites, chemically modified with collagen and reinforced by bioactive glass nanoparticles (BG) on the development of injectable nanohybrids for regenerative medicine applications.
27238483	0	0	theme	Intestine	90:98	arg1	Heparin					100:106	Porcine Intestine Heparin	82:106	Porcine Intestine Heparin	82:106	Comparison of Low-Molecular-Weight Heparins Prepared From Bovine Lung Heparin and Porcine Intestine Heparin.
27238483	6	1	theme	2-dimensional	987:999	arg1	resonance					1018:1026	2-dimensional nuclear magnetic resonance	987:1026	2-dimensional nuclear magnetic resonance spectroscopy	987:1039	Three batches of BLEs were prepared and their detailed structures were compared with PIEs using modern analytical techniques, including disaccharide composition, intact chain mapping by liquid chromatography-mass spectrometry and 2-dimensional nuclear magnetic resonance spectroscopy.
27238483	1	2	from	heparin	194:200	arg1	countries					210:218	most countries	205:218	most countries	205:218	Currently porcine intestine is the only approved source for producing pharmaceutical heparin in most countries.
27238483	2	3	from	prevalent	322:330	arg1	market					358:363	the anticoagulant drug market	335:363	the anticoagulant drug market	335:363	Enoxaparin, prepared by benzylation and alkaline depolymerization from porcine intestine heparin, is prevalent in the anticoagulant drug market.
27238483	4	4	theme	potential	597:605	arg1	source					619:624	a potential alternative source	595:624	a potential alternative source for producing enoxaparin	595:649	Bovine lung heparin is a potential alternative source for producing enoxaparin.
27238483	4	4	theme	potential	597:605	arg1	heparin					584:590	Bovine lung heparin	572:590	Bovine lung heparin	572:590	Bovine lung heparin is a potential alternative source for producing enoxaparin.
27238483	0	5	theme	Porcine	82:88	arg1	Heparin					100:106	Porcine Intestine Heparin	82:106	Porcine Intestine Heparin	82:106	Comparison of Low-Molecular-Weight Heparins Prepared From Bovine Lung Heparin and Porcine Intestine Heparin.
27238483	5	6	theme	bovine	697:702	arg1	BLE					737:739	BLE	737:739	BLE	737:739	Critical processing parameters for producing bovine lung heparin-derived enoxaparin (BLE) are discussed.
27238483	5	6	theme	bovine	697:702	arg1	enoxaparin					725:734	bovine lung heparin-derived enoxaparin	697:734	bovine lung heparin-derived enoxaparin (BLE)	697:740	Critical processing parameters for producing bovine lung heparin-derived enoxaparin (BLE) are discussed.
27238483	2	7	from	market	358:363	arg1	prevalent					322:330	prevalent	322:330	prevalent	322:330	Enoxaparin, prepared by benzylation and alkaline depolymerization from porcine intestine heparin, is prevalent in the anticoagulant drug market.
27238483	5	8	theme	lung	704:707	arg1	BLE					737:739	BLE	737:739	BLE	737:739	Critical processing parameters for producing bovine lung heparin-derived enoxaparin (BLE) are discussed.
27238483	5	8	theme	lung	704:707	arg1	enoxaparin					725:734	bovine lung heparin-derived enoxaparin	697:734	bovine lung heparin-derived enoxaparin (BLE)	697:740	Critical processing parameters for producing bovine lung heparin-derived enoxaparin (BLE) are discussed.
27238483	6	9	theme	modern	853:858	arg1	spectroscopy					1028:1039	2-dimensional nuclear magnetic resonance spectroscopy	987:1039	2-dimensional nuclear magnetic resonance spectroscopy	987:1039	Three batches of BLEs were prepared and their detailed structures were compared with PIEs using modern analytical techniques, including disaccharide composition, intact chain mapping by liquid chromatography-mass spectrometry and 2-dimensional nuclear magnetic resonance spectroscopy.
27238483	6	9	theme	modern	853:858	arg1	techniques					871:880	modern analytical techniques	853:880	modern analytical techniques	853:880	Three batches of BLEs were prepared and their detailed structures were compared with PIEs using modern analytical techniques, including disaccharide composition, intact chain mapping by liquid chromatography-mass spectrometry and 2-dimensional nuclear magnetic resonance spectroscopy.
27238483	6	9	theme	modern	853:858	arg1	mapping					932:938	intact chain mapping	919:938	intact chain mapping by liquid chromatography-mass spectrometry	919:981	Three batches of BLEs were prepared and their detailed structures were compared with PIEs using modern analytical techniques, including disaccharide composition, intact chain mapping by liquid chromatography-mass spectrometry and 2-dimensional nuclear magnetic resonance spectroscopy.
27238483	6	9	theme	modern	853:858	arg1	composition					906:916	disaccharide composition	893:916	disaccharide composition	893:916	Three batches of BLEs were prepared and their detailed structures were compared with PIEs using modern analytical techniques, including disaccharide composition, intact chain mapping by liquid chromatography-mass spectrometry and 2-dimensional nuclear magnetic resonance spectroscopy.
27238483	3	10	theme	porcine	387:393	arg1	intestine					395:403	porcine intestine	387:403	porcine intestine heparin-derived enoxaparin (PIE)	387:436	It is predicted that porcine intestine heparin-derived enoxaparin (PIE) will encounter shortage, and expanding its production from heparins obtained from other animal tissues may, therefore, be inevitable.
27238483	6	11	theme	chromatography-mass	950:968	arg1	spectrometry					970:981	liquid chromatography-mass spectrometry	943:981	liquid chromatography-mass spectrometry	943:981	Three batches of BLEs were prepared and their detailed structures were compared with PIEs using modern analytical techniques, including disaccharide composition, intact chain mapping by liquid chromatography-mass spectrometry and 2-dimensional nuclear magnetic resonance spectroscopy.
27238483	5	12	theme	heparin-derived	709:723	arg1	BLE					737:739	BLE	737:739	BLE	737:739	Critical processing parameters for producing bovine lung heparin-derived enoxaparin (BLE) are discussed.
27238483	5	12	theme	heparin-derived	709:723	arg1	enoxaparin					725:734	bovine lung heparin-derived enoxaparin	697:734	bovine lung heparin-derived enoxaparin (BLE)	697:740	Critical processing parameters for producing bovine lung heparin-derived enoxaparin (BLE) are discussed.
27238483	7	13	attach	derived	1152:1158	arg1	heparins					1176:1183	the parent heparins	1165:1183	the parent heparins	1165:1183	The results suggested that the differences between PIEs and BLEs mainly result from N-acetylation differences derived from the parent heparins.
27238483	7	13	attach	derived	1152:1158	arg2	differences					1140:1150	N-acetylation differences	1126:1150	N-acetylation differences derived from the parent heparins	1126:1183	The results suggested that the differences between PIEs and BLEs mainly result from N-acetylation differences derived from the parent heparins.
27238483	4	14	theme	alternative	607:617	arg1	source					619:624	a potential alternative source	595:624	a potential alternative source for producing enoxaparin	595:649	Bovine lung heparin is a potential alternative source for producing enoxaparin.
27238483	4	14	theme	alternative	607:617	arg1	heparin					584:590	Bovine lung heparin	572:590	Bovine lung heparin	572:590	Bovine lung heparin is a potential alternative source for producing enoxaparin.
27238483	8	15	theme	anti-factor	1253:1263	arg1	IIa					1265:1267	anti-factor IIa	1253:1267	anti-factor IIa	1253:1267	In addition, bioactivities of BLEs were about 70% of PIEs based on anti-factor IIa and Xa chromogenic assays.
27238483	6	16	theme	liquid	943:948	arg1	spectrometry					970:981	liquid chromatography-mass spectrometry	943:981	liquid chromatography-mass spectrometry	943:981	Three batches of BLEs were prepared and their detailed structures were compared with PIEs using modern analytical techniques, including disaccharide composition, intact chain mapping by liquid chromatography-mass spectrometry and 2-dimensional nuclear magnetic resonance spectroscopy.
27238483	5	17	theme	Critical	652:659	arg1	parameters					672:681	Critical processing parameters	652:681	Critical processing parameters for producing bovine lung heparin-derived enoxaparin (BLE)	652:740	Critical processing parameters for producing bovine lung heparin-derived enoxaparin (BLE) are discussed.
27238483	8	18	theme	Xa	1273:1274	arg1	assays					1288:1293	Xa chromogenic assays	1273:1293	Xa chromogenic assays	1273:1293	In addition, bioactivities of BLEs were about 70% of PIEs based on anti-factor IIa and Xa chromogenic assays.
27238483	1	19	theme	pharmaceutical	179:192	arg1	heparin					194:200	pharmaceutical heparin	179:200	pharmaceutical heparin in most countries	179:218	Currently porcine intestine is the only approved source for producing pharmaceutical heparin in most countries.
27238483	5	20	theme	processing	661:670	arg1	parameters					672:681	Critical processing parameters	652:681	Critical processing parameters for producing bovine lung heparin-derived enoxaparin (BLE)	652:740	Critical processing parameters for producing bovine lung heparin-derived enoxaparin (BLE) are discussed.
27238483	2	21	theme	intestine	300:308	arg1	heparin					310:316	porcine intestine heparin	292:316	porcine intestine heparin	292:316	Enoxaparin, prepared by benzylation and alkaline depolymerization from porcine intestine heparin, is prevalent in the anticoagulant drug market.
27238483	2	22	theme	anticoagulant	339:351	arg1	market					358:363	the anticoagulant drug market	335:363	the anticoagulant drug market	335:363	Enoxaparin, prepared by benzylation and alkaline depolymerization from porcine intestine heparin, is prevalent in the anticoagulant drug market.
27238483	4	23	theme	lung	579:582	arg1	source					619:624	a potential alternative source	595:624	a potential alternative source for producing enoxaparin	595:649	Bovine lung heparin is a potential alternative source for producing enoxaparin.
27238483	4	23	theme	lung	579:582	arg1	heparin					584:590	Bovine lung heparin	572:590	Bovine lung heparin	572:590	Bovine lung heparin is a potential alternative source for producing enoxaparin.
27238483	1	24	theme	most	205:208	arg1	countries					210:218	most countries	205:218	most countries	205:218	Currently porcine intestine is the only approved source for producing pharmaceutical heparin in most countries.
27238483	3	25	theme	intestine	395:403	arg1	enoxaparin					421:430	porcine intestine heparin-derived enoxaparin	387:430	porcine intestine heparin-derived enoxaparin (PIE)	387:436	It is predicted that porcine intestine heparin-derived enoxaparin (PIE) will encounter shortage, and expanding its production from heparins obtained from other animal tissues may, therefore, be inevitable.
27238483	3	25	theme	intestine	395:403	arg1	PIE					433:435	PIE	433:435	PIE	433:435	It is predicted that porcine intestine heparin-derived enoxaparin (PIE) will encounter shortage, and expanding its production from heparins obtained from other animal tissues may, therefore, be inevitable.
27238483	4	26	theme	Bovine	572:577	arg1	source					619:624	a potential alternative source	595:624	a potential alternative source for producing enoxaparin	595:649	Bovine lung heparin is a potential alternative source for producing enoxaparin.
27238483	4	26	theme	Bovine	572:577	arg1	heparin					584:590	Bovine lung heparin	572:590	Bovine lung heparin	572:590	Bovine lung heparin is a potential alternative source for producing enoxaparin.
27238483	7	27	theme	parent	1169:1174	arg1	heparins					1176:1183	the parent heparins	1165:1183	the parent heparins	1165:1183	The results suggested that the differences between PIEs and BLEs mainly result from N-acetylation differences derived from the parent heparins.
27238483	8	28	theme	chromogenic	1276:1286	arg1	assays					1288:1293	Xa chromogenic assays	1273:1293	Xa chromogenic assays	1273:1293	In addition, bioactivities of BLEs were about 70% of PIEs based on anti-factor IIa and Xa chromogenic assays.
27238483	6	29	theme	analytical	860:869	arg1	spectroscopy					1028:1039	2-dimensional nuclear magnetic resonance spectroscopy	987:1039	2-dimensional nuclear magnetic resonance spectroscopy	987:1039	Three batches of BLEs were prepared and their detailed structures were compared with PIEs using modern analytical techniques, including disaccharide composition, intact chain mapping by liquid chromatography-mass spectrometry and 2-dimensional nuclear magnetic resonance spectroscopy.
27238483	6	29	theme	analytical	860:869	arg1	techniques					871:880	modern analytical techniques	853:880	modern analytical techniques	853:880	Three batches of BLEs were prepared and their detailed structures were compared with PIEs using modern analytical techniques, including disaccharide composition, intact chain mapping by liquid chromatography-mass spectrometry and 2-dimensional nuclear magnetic resonance spectroscopy.
27238483	6	29	theme	analytical	860:869	arg1	mapping					932:938	intact chain mapping	919:938	intact chain mapping by liquid chromatography-mass spectrometry	919:981	Three batches of BLEs were prepared and their detailed structures were compared with PIEs using modern analytical techniques, including disaccharide composition, intact chain mapping by liquid chromatography-mass spectrometry and 2-dimensional nuclear magnetic resonance spectroscopy.
27238483	6	29	theme	analytical	860:869	arg1	composition					906:916	disaccharide composition	893:916	disaccharide composition	893:916	Three batches of BLEs were prepared and their detailed structures were compared with PIEs using modern analytical techniques, including disaccharide composition, intact chain mapping by liquid chromatography-mass spectrometry and 2-dimensional nuclear magnetic resonance spectroscopy.
27238483	3	30	theme	heparin-derived	405:419	arg1	enoxaparin					421:430	porcine intestine heparin-derived enoxaparin	387:430	porcine intestine heparin-derived enoxaparin (PIE)	387:436	It is predicted that porcine intestine heparin-derived enoxaparin (PIE) will encounter shortage, and expanding its production from heparins obtained from other animal tissues may, therefore, be inevitable.
27238483	3	30	theme	heparin-derived	405:419	arg1	PIE					433:435	PIE	433:435	PIE	433:435	It is predicted that porcine intestine heparin-derived enoxaparin (PIE) will encounter shortage, and expanding its production from heparins obtained from other animal tissues may, therefore, be inevitable.
27238483	6	31	theme	chain	926:930	arg1	mapping					932:938	intact chain mapping	919:938	intact chain mapping by liquid chromatography-mass spectrometry	919:981	Three batches of BLEs were prepared and their detailed structures were compared with PIEs using modern analytical techniques, including disaccharide composition, intact chain mapping by liquid chromatography-mass spectrometry and 2-dimensional nuclear magnetic resonance spectroscopy.
27238483	1	32	theme	porcine	119:125	arg1	source					158:163	the only approved source	140:163	the only approved source for producing pharmaceutical heparin in most countries	140:218	Currently porcine intestine is the only approved source for producing pharmaceutical heparin in most countries.
27238483	1	32	theme	porcine	119:125	arg1	intestine					127:135	porcine intestine	119:135	porcine intestine	119:135	Currently porcine intestine is the only approved source for producing pharmaceutical heparin in most countries.
27238483	5	33	link	heparin-derived	709:723	arg1	BLE					737:739	BLE	737:739	BLE	737:739	Critical processing parameters for producing bovine lung heparin-derived enoxaparin (BLE) are discussed.
27238483	5	33	link	heparin-derived	709:723	arg1	enoxaparin					725:734	bovine lung heparin-derived enoxaparin	697:734	bovine lung heparin-derived enoxaparin (BLE)	697:740	Critical processing parameters for producing bovine lung heparin-derived enoxaparin (BLE) are discussed.
27238483	6	34	theme	intact	919:924	arg1	mapping					932:938	intact chain mapping	919:938	intact chain mapping by liquid chromatography-mass spectrometry	919:981	Three batches of BLEs were prepared and their detailed structures were compared with PIEs using modern analytical techniques, including disaccharide composition, intact chain mapping by liquid chromatography-mass spectrometry and 2-dimensional nuclear magnetic resonance spectroscopy.
27238483	0	35	theme	Low-Molecular-Weight	14:33	arg1	Heparins					35:42	Low-Molecular-Weight Heparins	14:42	Low-Molecular-Weight Heparins	14:42	Comparison of Low-Molecular-Weight Heparins Prepared From Bovine Lung Heparin and Porcine Intestine Heparin.
27238483	9	36	theme	PIE	1369:1371	arg1	analogue					1357:1364	an analogue	1354:1364	an analogue of PIE	1354:1371	We conclude that BLE has the potential to be developed as an analogue of PIE, although some challenges still remain.
27238483	0	37	theme	Heparins	35:42	arg1	Comparison					0:9	Comparison	0:9	Comparison of Low-Molecular-Weight Heparins	0:42	Comparison of Low-Molecular-Weight Heparins Prepared From Bovine Lung Heparin and Porcine Intestine Heparin.
27238483	6	38	theme	detailed	803:810	arg1	structures					812:821	their detailed structures	797:821	their detailed structures	797:821	Three batches of BLEs were prepared and their detailed structures were compared with PIEs using modern analytical techniques, including disaccharide composition, intact chain mapping by liquid chromatography-mass spectrometry and 2-dimensional nuclear magnetic resonance spectroscopy.
27238483	0	39	theme	Lung	65:68	arg1	Heparin					70:76	Bovine Lung Heparin	58:76	Bovine Lung Heparin	58:76	Comparison of Low-Molecular-Weight Heparins Prepared From Bovine Lung Heparin and Porcine Intestine Heparin.
27238483	3	40	theme	other	520:524	arg1	tissues					533:539	other animal tissues	520:539	other animal tissues	520:539	It is predicted that porcine intestine heparin-derived enoxaparin (PIE) will encounter shortage, and expanding its production from heparins obtained from other animal tissues may, therefore, be inevitable.
27238483	0	41	theme	Bovine	58:63	arg1	Heparin					70:76	Bovine Lung Heparin	58:76	Bovine Lung Heparin	58:76	Comparison of Low-Molecular-Weight Heparins Prepared From Bovine Lung Heparin and Porcine Intestine Heparin.
27238483	6	42	theme	resonance	1018:1026	arg1	spectroscopy					1028:1039	2-dimensional nuclear magnetic resonance spectroscopy	987:1039	2-dimensional nuclear magnetic resonance spectroscopy	987:1039	Three batches of BLEs were prepared and their detailed structures were compared with PIEs using modern analytical techniques, including disaccharide composition, intact chain mapping by liquid chromatography-mass spectrometry and 2-dimensional nuclear magnetic resonance spectroscopy.
27238483	6	43	theme	disaccharide	893:904	arg1	composition					906:916	disaccharide composition	893:916	disaccharide composition	893:916	Three batches of BLEs were prepared and their detailed structures were compared with PIEs using modern analytical techniques, including disaccharide composition, intact chain mapping by liquid chromatography-mass spectrometry and 2-dimensional nuclear magnetic resonance spectroscopy.
27238483	3	44	theme	animal	526:531	arg1	tissues					533:539	other animal tissues	520:539	other animal tissues	520:539	It is predicted that porcine intestine heparin-derived enoxaparin (PIE) will encounter shortage, and expanding its production from heparins obtained from other animal tissues may, therefore, be inevitable.
27238483	6	45	theme	magnetic	1009:1016	arg1	resonance					1018:1026	2-dimensional nuclear magnetic resonance	987:1026	2-dimensional nuclear magnetic resonance spectroscopy	987:1039	Three batches of BLEs were prepared and their detailed structures were compared with PIEs using modern analytical techniques, including disaccharide composition, intact chain mapping by liquid chromatography-mass spectrometry and 2-dimensional nuclear magnetic resonance spectroscopy.
27238483	2	46	theme	porcine	292:298	arg1	heparin					310:316	porcine intestine heparin	292:316	porcine intestine heparin	292:316	Enoxaparin, prepared by benzylation and alkaline depolymerization from porcine intestine heparin, is prevalent in the anticoagulant drug market.
27238483	3	47	from	heparins	497:504	arg1	production					481:490	its production	477:490	its production from heparins obtained from other animal tissues	477:539	It is predicted that porcine intestine heparin-derived enoxaparin (PIE) will encounter shortage, and expanding its production from heparins obtained from other animal tissues may, therefore, be inevitable.
27238483	9	48	contain	has	1317:1319	arg1	BLE					1313:1315	BLE	1313:1315	BLE	1313:1315	We conclude that BLE has the potential to be developed as an analogue of PIE, although some challenges still remain.
27238483	9	48	contain	has	1317:1319	arg2	potential					1325:1333	the potential to be developed as an analogue of PIE	1321:1371	the potential to be developed as an analogue of PIE	1321:1371	We conclude that BLE has the potential to be developed as an analogue of PIE, although some challenges still remain.
27238483	7	49	theme	N-acetylation	1126:1138	arg1	differences					1140:1150	N-acetylation differences	1126:1150	N-acetylation differences derived from the parent heparins	1126:1183	The results suggested that the differences between PIEs and BLEs mainly result from N-acetylation differences derived from the parent heparins.
27238483	3	50	link	heparin-derived	405:419	arg1	enoxaparin					421:430	porcine intestine heparin-derived enoxaparin	387:430	porcine intestine heparin-derived enoxaparin (PIE)	387:436	It is predicted that porcine intestine heparin-derived enoxaparin (PIE) will encounter shortage, and expanding its production from heparins obtained from other animal tissues may, therefore, be inevitable.
27238483	3	50	link	heparin-derived	405:419	arg1	PIE					433:435	PIE	433:435	PIE	433:435	It is predicted that porcine intestine heparin-derived enoxaparin (PIE) will encounter shortage, and expanding its production from heparins obtained from other animal tissues may, therefore, be inevitable.
27238483	2	51	theme	drug	353:356	arg1	market					358:363	the anticoagulant drug market	335:363	the anticoagulant drug market	335:363	Enoxaparin, prepared by benzylation and alkaline depolymerization from porcine intestine heparin, is prevalent in the anticoagulant drug market.
27238483	8	52	from	%	1234:1234	arg1	addition					1189:1196	addition	1189:1196	addition	1189:1196	In addition, bioactivities of BLEs were about 70% of PIEs based on anti-factor IIa and Xa chromogenic assays.
27238483	6	53	theme	BLEs	774:777	arg1	batches					763:769	Three batches	757:769	Three batches of BLEs	757:777	Three batches of BLEs were prepared and their detailed structures were compared with PIEs using modern analytical techniques, including disaccharide composition, intact chain mapping by liquid chromatography-mass spectrometry and 2-dimensional nuclear magnetic resonance spectroscopy.
27238483	1	54	theme	approved	149:156	arg1	source					158:163	the only approved source	140:163	the only approved source for producing pharmaceutical heparin in most countries	140:218	Currently porcine intestine is the only approved source for producing pharmaceutical heparin in most countries.
27238483	1	54	theme	approved	149:156	arg1	intestine					127:135	porcine intestine	119:135	porcine intestine	119:135	Currently porcine intestine is the only approved source for producing pharmaceutical heparin in most countries.
27238483	6	55	theme	nuclear	1001:1007	arg1	resonance					1018:1026	2-dimensional nuclear magnetic resonance	987:1026	2-dimensional nuclear magnetic resonance spectroscopy	987:1039	Three batches of BLEs were prepared and their detailed structures were compared with PIEs using modern analytical techniques, including disaccharide composition, intact chain mapping by liquid chromatography-mass spectrometry and 2-dimensional nuclear magnetic resonance spectroscopy.
27238483	2	56	theme	alkaline	261:268	arg1	depolymerization					270:285	alkaline depolymerization	261:285	alkaline depolymerization	261:285	Enoxaparin, prepared by benzylation and alkaline depolymerization from porcine intestine heparin, is prevalent in the anticoagulant drug market.
27238483	8	57	theme	BLEs	1216:1219	arg1	bioactivities					1199:1211	bioactivities	1199:1211	bioactivities of BLEs	1199:1219	In addition, bioactivities of BLEs were about 70% of PIEs based on anti-factor IIa and Xa chromogenic assays.
27238483	8	58	theme	PIEs	1239:1242	arg1	%					1234:1234	about 70%	1226:1234	about 70% of PIEs based on anti-factor IIa and Xa chromogenic assays	1226:1293	In addition, bioactivities of BLEs were about 70% of PIEs based on anti-factor IIa and Xa chromogenic assays.
27238483	8	58	theme	PIEs	1239:1242	arg1	PIEs					1239:1242	PIEs	1239:1242	PIEs based on anti-factor IIa and Xa chromogenic assays	1239:1293	In addition, bioactivities of BLEs were about 70% of PIEs based on anti-factor IIa and Xa chromogenic assays.
26794090	6	0	theme	same	1112:1115	arg1	type					1117:1120	the same type	1108:1120	the same type of tumors	1108:1130	RESULTS Ten glyco-genes (GALNT3, GALNT6, GALNT7, GALNT14, MGAT3, MAN1A1, MAN1C1, ST3GAL2, ST6GAL1, ST8SIA3) showing changes in both methylation and expression in the same type of cancer belong to GalNAc transferases, GlcNAc transferases, mannosidases and sialyltransferases, which is in line with changes in glycan structures already reported in the same type of tumors.
26794090	5	1	theme	lymph	729:733	arg1	node					735:738	lymph node	729:738	lymph node	729:738	We also analyzed methylation datasets without the available matching expression data for glyco-genes in lung cancer, and progression of melanoma into lymph node and brain metastases.
26794090	6	2	theme	same	928:931	arg1	type					933:936	the same type	924:936	the same type of cancer	924:946	RESULTS Ten glyco-genes (GALNT3, GALNT6, GALNT7, GALNT14, MGAT3, MAN1A1, MAN1C1, ST3GAL2, ST6GAL1, ST8SIA3) showing changes in both methylation and expression in the same type of cancer belong to GalNAc transferases, GlcNAc transferases, mannosidases and sialyltransferases, which is in line with changes in glycan structures already reported in the same type of tumors.
26794090	1	3	theme	covalent	122:129	arg1	addition					131:138	covalent addition	122:138	covalent addition of glycan molecules that considerably influence their function	122:201	BACKGROUND Most eukaryotic proteins are modified by covalent addition of glycan molecules that considerably influence their function.
26794090	4	4	theme	glyco-genes	464:474	arg1	expression					447:456	expression	447:456	expression	447:456	METHODS We analyzed DNA methylation and expression of 86 glyco-genes in melanoma, hepatocellular, breast and cervical cancers using data from publicly available databases.
26794090	4	4	theme	glyco-genes	464:474	arg1	methylation					431:441	DNA methylation	427:441	DNA methylation	427:441	METHODS We analyzed DNA methylation and expression of 86 glyco-genes in melanoma, hepatocellular, breast and cervical cancers using data from publicly available databases.
26794090	6	5	from	changes	1059:1065	arg1	structures					1077:1086	glycan structures	1070:1086	glycan structures already reported in the same type of tumors	1070:1130	RESULTS Ten glyco-genes (GALNT3, GALNT6, GALNT7, GALNT14, MGAT3, MAN1A1, MAN1C1, ST3GAL2, ST6GAL1, ST8SIA3) showing changes in both methylation and expression in the same type of cancer belong to GalNAc transferases, GlcNAc transferases, mannosidases and sialyltransferases, which is in line with changes in glycan structures already reported in the same type of tumors.
26794090	8	6	dep	other	1391:1395	arg1	than					1397:1400	than	1397:1400	than	1397:1400	The MGAT5B gene, so far identified as specifically expressed in brain, emerged as a novel candidate gene that is epigenetically dysregulated in different cancers other than brain cancer.
26794090	4	7	theme	DNA	427:429	arg1	methylation					431:441	DNA methylation	427:441	DNA methylation	427:441	METHODS We analyzed DNA methylation and expression of 86 glyco-genes in melanoma, hepatocellular, breast and cervical cancers using data from publicly available databases.
26794090	4	8	from	expression	447:456	arg1	hepatocellular					489:502	hepatocellular	489:502	hepatocellular	489:502	METHODS We analyzed DNA methylation and expression of 86 glyco-genes in melanoma, hepatocellular, breast and cervical cancers using data from publicly available databases.
26794090	4	8	from	expression	447:456	arg1	melanoma					479:486	melanoma	479:486	melanoma	479:486	METHODS We analyzed DNA methylation and expression of 86 glyco-genes in melanoma, hepatocellular, breast and cervical cancers using data from publicly available databases.
26794090	4	8	from	expression	447:456	arg1	breast					505:510	breast	505:510	breast	505:510	METHODS We analyzed DNA methylation and expression of 86 glyco-genes in melanoma, hepatocellular, breast and cervical cancers using data from publicly available databases.
26794090	4	8	from	expression	447:456	arg1	cancers					525:531	cervical cancers	516:531	cervical cancers	516:531	METHODS We analyzed DNA methylation and expression of 86 glyco-genes in melanoma, hepatocellular, breast and cervical cancers using data from publicly available databases.
26794090	6	9	from	changes	878:884	arg1	type					933:936	the same type	924:936	the same type of cancer	924:946	RESULTS Ten glyco-genes (GALNT3, GALNT6, GALNT7, GALNT14, MGAT3, MAN1A1, MAN1C1, ST3GAL2, ST6GAL1, ST8SIA3) showing changes in both methylation and expression in the same type of cancer belong to GalNAc transferases, GlcNAc transferases, mannosidases and sialyltransferases, which is in line with changes in glycan structures already reported in the same type of tumors.
26794090	6	9	from	changes	878:884	arg1	expression					910:919	expression	910:919	expression	910:919	RESULTS Ten glyco-genes (GALNT3, GALNT6, GALNT7, GALNT14, MGAT3, MAN1A1, MAN1C1, ST3GAL2, ST6GAL1, ST8SIA3) showing changes in both methylation and expression in the same type of cancer belong to GalNAc transferases, GlcNAc transferases, mannosidases and sialyltransferases, which is in line with changes in glycan structures already reported in the same type of tumors.
26794090	6	9	from	changes	878:884	arg1	methylation					894:904	methylation	894:904	methylation	894:904	RESULTS Ten glyco-genes (GALNT3, GALNT6, GALNT7, GALNT14, MGAT3, MAN1A1, MAN1C1, ST3GAL2, ST6GAL1, ST8SIA3) showing changes in both methylation and expression in the same type of cancer belong to GalNAc transferases, GlcNAc transferases, mannosidases and sialyltransferases, which is in line with changes in glycan structures already reported in the same type of tumors.
26794090	5	10	theme	melanoma	715:722	arg1	datasets					608:615	methylation datasets	596:615	methylation datasets without the available matching expression data for glyco-genes in lung cancer	596:693	We also analyzed methylation datasets without the available matching expression data for glyco-genes in lung cancer, and progression of melanoma into lymph node and brain metastases.
26794090	5	10	theme	melanoma	715:722	arg1	progression					700:710	progression	700:710	progression of melanoma into lymph node and brain metastases	700:759	We also analyzed methylation datasets without the available matching expression data for glyco-genes in lung cancer, and progression of melanoma into lymph node and brain metastases.
26794090	3	11	theme	glycan	318:323	arg1	structures					325:334	Some glycan structures	313:334	Some glycan structures	313:334	Some glycan structures are tumor-specific and reflect disturbed glycan biosynthesis pathways.
26794090	9	12	from	genes	1491:1495	arg1	cancer					1500:1505	cancer	1500:1505	cancer	1500:1505	We also report for the first time aberrant expression of the GALNT and MAN genes in cancer by aberrant promoter methylation.
26794090	4	13	from	methylation	431:441	arg1	hepatocellular					489:502	hepatocellular	489:502	hepatocellular	489:502	METHODS We analyzed DNA methylation and expression of 86 glyco-genes in melanoma, hepatocellular, breast and cervical cancers using data from publicly available databases.
26794090	4	13	from	methylation	431:441	arg1	melanoma					479:486	melanoma	479:486	melanoma	479:486	METHODS We analyzed DNA methylation and expression of 86 glyco-genes in melanoma, hepatocellular, breast and cervical cancers using data from publicly available databases.
26794090	4	13	from	methylation	431:441	arg1	breast					505:510	breast	505:510	breast	505:510	METHODS We analyzed DNA methylation and expression of 86 glyco-genes in melanoma, hepatocellular, breast and cervical cancers using data from publicly available databases.
26794090	4	13	from	methylation	431:441	arg1	cancers					525:531	cervical cancers	516:531	cervical cancers	516:531	METHODS We analyzed DNA methylation and expression of 86 glyco-genes in melanoma, hepatocellular, breast and cervical cancers using data from publicly available databases.
26794090	10	14	theme	aberrant	1595:1602	arg1	methylation					1613:1623	aberrant promoter methylation	1595:1623	aberrant promoter methylation	1595:1623	CONCLUSIONS Aberrant expression of glyco-genes due to aberrant promoter methylation could be a way leading to characteristic glycosylation profiles commonly described in cancer.
26794090	11	15	theme	specific	1835:1842	arg1	tumors					1844:1849	specific tumors	1835:1849	specific tumors	1835:1849	GENERAL SIGNIFICANCE Methylation status in promoters of candidate glyco-genes might serve as prognostic markers for specific tumors and point to potential novel targets for epigenetic drugs.
26794090	8	16	theme	MGAT5B	1233:1238	arg1	gene					1240:1243	The MGAT5B gene	1229:1243	The MGAT5B gene	1229:1243	The MGAT5B gene, so far identified as specifically expressed in brain, emerged as a novel candidate gene that is epigenetically dysregulated in different cancers other than brain cancer.
26794090	5	17	from	glyco-genes	668:678	arg1	cancer					688:693	lung cancer	683:693	lung cancer	683:693	We also analyzed methylation datasets without the available matching expression data for glyco-genes in lung cancer, and progression of melanoma into lymph node and brain metastases.
26794090	12	18	dep	Glycans	1960:1966	arg1	Lauc					2025:2028	Lauc	2025:2028	Lauc	2025:2028	This article is part of a Special Issue entitled "Glycans in personalised medicine" Guest Editor: Professor Gordan Lauc.
26794090	11	19	theme	novel	1874:1878	arg1	targets					1880:1886	potential novel targets	1864:1886	potential novel targets for epigenetic drugs	1864:1907	GENERAL SIGNIFICANCE Methylation status in promoters of candidate glyco-genes might serve as prognostic markers for specific tumors and point to potential novel targets for epigenetic drugs.
26794090	4	20	from	breast	505:510	arg1	expression					447:456	expression	447:456	expression	447:456	METHODS We analyzed DNA methylation and expression of 86 glyco-genes in melanoma, hepatocellular, breast and cervical cancers using data from publicly available databases.
26794090	4	20	from	breast	505:510	arg1	methylation					431:441	DNA methylation	427:441	DNA methylation	427:441	METHODS We analyzed DNA methylation and expression of 86 glyco-genes in melanoma, hepatocellular, breast and cervical cancers using data from publicly available databases.
26794090	12	21	theme	Guest	1994:1998	arg1	Editor					2000:2005	Guest Editor	1994:2005	Guest Editor	1994:2005	This article is part of a Special Issue entitled "Glycans in personalised medicine" Guest Editor: Professor Gordan Lauc.
26794090	6	22	theme	GalNAc	958:963	arg1	transferases					965:976	GalNAc transferases	958:976	GalNAc transferases	958:976	RESULTS Ten glyco-genes (GALNT3, GALNT6, GALNT7, GALNT14, MGAT3, MAN1A1, MAN1C1, ST3GAL2, ST6GAL1, ST8SIA3) showing changes in both methylation and expression in the same type of cancer belong to GalNAc transferases, GlcNAc transferases, mannosidases and sialyltransferases, which is in line with changes in glycan structures already reported in the same type of tumors.
26794090	4	23	theme	cervical	516:523	arg1	cancers					525:531	cervical cancers	516:531	cervical cancers	516:531	METHODS We analyzed DNA methylation and expression of 86 glyco-genes in melanoma, hepatocellular, breast and cervical cancers using data from publicly available databases.
26794090	6	24	with	line	1049:1052	arg1	changes					1059:1065	changes	1059:1065	changes in glycan structures already reported in the same type of tumors	1059:1130	RESULTS Ten glyco-genes (GALNT3, GALNT6, GALNT7, GALNT14, MGAT3, MAN1A1, MAN1C1, ST3GAL2, ST6GAL1, ST8SIA3) showing changes in both methylation and expression in the same type of cancer belong to GalNAc transferases, GlcNAc transferases, mannosidases and sialyltransferases, which is in line with changes in glycan structures already reported in the same type of tumors.
26794090	4	25	from	hepatocellular	489:502	arg1	expression					447:456	expression	447:456	expression	447:456	METHODS We analyzed DNA methylation and expression of 86 glyco-genes in melanoma, hepatocellular, breast and cervical cancers using data from publicly available databases.
26794090	4	25	from	hepatocellular	489:502	arg1	methylation					431:441	DNA methylation	427:441	DNA methylation	427:441	METHODS We analyzed DNA methylation and expression of 86 glyco-genes in melanoma, hepatocellular, breast and cervical cancers using data from publicly available databases.
26794090	5	26	theme	available	629:637	arg1	data					659:662	the available matching expression data	625:662	the available matching expression data for glyco-genes in lung cancer	625:693	We also analyzed methylation datasets without the available matching expression data for glyco-genes in lung cancer, and progression of melanoma into lymph node and brain metastases.
26794090	9	27	theme	genes	1491:1495	arg1	expression					1459:1468	aberrant expression	1450:1468	aberrant expression of the GALNT and MAN genes in cancer by aberrant promoter methylation	1450:1538	We also report for the first time aberrant expression of the GALNT and MAN genes in cancer by aberrant promoter methylation.
26794090	4	28	from	melanoma	479:486	arg1	expression					447:456	expression	447:456	expression	447:456	METHODS We analyzed DNA methylation and expression of 86 glyco-genes in melanoma, hepatocellular, breast and cervical cancers using data from publicly available databases.
26794090	4	28	from	melanoma	479:486	arg1	methylation					431:441	DNA methylation	427:441	DNA methylation	427:441	METHODS We analyzed DNA methylation and expression of 86 glyco-genes in melanoma, hepatocellular, breast and cervical cancers using data from publicly available databases.
26794090	1	29	theme	glycan	143:148	arg1	molecules					150:158	glycan molecules	143:158	glycan molecules that considerably influence their function	143:201	BACKGROUND Most eukaryotic proteins are modified by covalent addition of glycan molecules that considerably influence their function.
26794090	12	30	from	Glycans	1960:1966	arg1	medicine					1984:1991	personalised medicine	1971:1991	personalised medicine" Guest Editor	1971:2005	This article is part of a Special Issue entitled "Glycans in personalised medicine" Guest Editor: Professor Gordan Lauc.
26794090	3	31	theme	biosynthesis	384:395	arg1	pathways					397:404	disturbed glycan biosynthesis pathways	367:404	disturbed glycan biosynthesis pathways	367:404	Some glycan structures are tumor-specific and reflect disturbed glycan biosynthesis pathways.
26794090	8	32	theme	candidate	1319:1327	arg1	gene					1329:1332	a novel candidate gene	1311:1332	a novel candidate gene that is epigenetically dysregulated in different cancers other than brain cancer	1311:1413	The MGAT5B gene, so far identified as specifically expressed in brain, emerged as a novel candidate gene that is epigenetically dysregulated in different cancers other than brain cancer.
26794090	11	33	theme	candidate	1775:1783	arg1	glyco-genes					1785:1795	candidate glyco-genes	1775:1795	candidate glyco-genes	1775:1795	GENERAL SIGNIFICANCE Methylation status in promoters of candidate glyco-genes might serve as prognostic markers for specific tumors and point to potential novel targets for epigenetic drugs.
26794090	2	34	theme	tumor	279:283	arg1	progression					285:295	tumor progression	279:295	tumor progression	279:295	Aberrant glycosylation is profoundly involved in malignant transformation, tumor progression and metastasis.
26794090	5	35	theme	methylation	596:606	arg1	datasets					608:615	methylation datasets	596:615	methylation datasets without the available matching expression data for glyco-genes in lung cancer	596:693	We also analyzed methylation datasets without the available matching expression data for glyco-genes in lung cancer, and progression of melanoma into lymph node and brain metastases.
26794090	9	36	theme	aberrant	1510:1517	arg1	methylation					1528:1538	aberrant promoter methylation	1510:1538	aberrant promoter methylation	1510:1538	We also report for the first time aberrant expression of the GALNT and MAN genes in cancer by aberrant promoter methylation.
26794090	3	37	theme	disturbed	367:375	arg1	pathways					397:404	disturbed glycan biosynthesis pathways	367:404	disturbed glycan biosynthesis pathways	367:404	Some glycan structures are tumor-specific and reflect disturbed glycan biosynthesis pathways.
26794090	0	38	from	expression	19:28	arg1	cancer					33:38	cancer	33:38	cancer	33:38	Glyco-genes change expression in cancer through aberrant methylation.
26794090	2	39	theme	Aberrant	204:211	arg1	glycosylation					213:225	Aberrant glycosylation	204:225	Aberrant glycosylation	204:225	Aberrant glycosylation is profoundly involved in malignant transformation, tumor progression and metastasis.
26794090	1	40	theme	BACKGROUND	70:79	arg1	proteins					97:104	BACKGROUND Most eukaryotic proteins	70:104	BACKGROUND Most eukaryotic proteins	70:104	BACKGROUND Most eukaryotic proteins are modified by covalent addition of glycan molecules that considerably influence their function.
26794090	5	41	theme	expression	648:657	arg1	data					659:662	the available matching expression data	625:662	the available matching expression data for glyco-genes in lung cancer	625:693	We also analyzed methylation datasets without the available matching expression data for glyco-genes in lung cancer, and progression of melanoma into lymph node and brain metastases.
26794090	1	42	theme	eukaryotic	86:95	arg1	proteins					97:104	BACKGROUND Most eukaryotic proteins	70:104	BACKGROUND Most eukaryotic proteins	70:104	BACKGROUND Most eukaryotic proteins are modified by covalent addition of glycan molecules that considerably influence their function.
26794090	11	43	from	status	1752:1757	arg1	promoters					1762:1770	promoters	1762:1770	promoters of candidate glyco-genes	1762:1795	GENERAL SIGNIFICANCE Methylation status in promoters of candidate glyco-genes might serve as prognostic markers for specific tumors and point to potential novel targets for epigenetic drugs.
26794090	10	44	theme	characteristic	1651:1664	arg1	profiles					1680:1687	characteristic glycosylation profiles	1651:1687	characteristic glycosylation profiles commonly described in cancer	1651:1716	CONCLUSIONS Aberrant expression of glyco-genes due to aberrant promoter methylation could be a way leading to characteristic glycosylation profiles commonly described in cancer.
26794090	7	45	theme	valuable	1197:1204	arg1	prognosis					1218:1226	potentially valuable for disease prognosis	1185:1226	potentially valuable for disease prognosis	1185:1226	Some of those genes were additionally identified as potentially valuable for disease prognosis.
26794090	7	45	theme	valuable	1197:1204	arg1	Some					1133:1136	Some	1133:1136	Some	1133:1136	Some of those genes were additionally identified as potentially valuable for disease prognosis.
26794090	7	45	theme	valuable	1197:1204	arg1	genes					1147:1151	those genes	1141:1151	those genes	1141:1151	Some of those genes were additionally identified as potentially valuable for disease prognosis.
26794090	12	46	theme	Special	1936:1942	arg1	Issue					1944:1948	a Special Issue	1934:1948	a Special Issue entitled "Glycans in personalised medicine" Guest Editor: Professor Gordan Lauc	1934:2028	This article is part of a Special Issue entitled "Glycans in personalised medicine" Guest Editor: Professor Gordan Lauc.
26794090	11	47	theme	Methylation	1740:1750	arg1	markers					1823:1829	prognostic markers	1812:1829	prognostic markers for specific tumors	1812:1849	GENERAL SIGNIFICANCE Methylation status in promoters of candidate glyco-genes might serve as prognostic markers for specific tumors and point to potential novel targets for epigenetic drugs.
26794090	11	47	theme	Methylation	1740:1750	arg1	status					1752:1757	GENERAL SIGNIFICANCE Methylation status	1719:1757	GENERAL SIGNIFICANCE Methylation status in promoters of candidate glyco-genes	1719:1795	GENERAL SIGNIFICANCE Methylation status in promoters of candidate glyco-genes might serve as prognostic markers for specific tumors and point to potential novel targets for epigenetic drugs.
26794090	11	48	theme	GENERAL	1719:1725	arg1	markers					1823:1829	prognostic markers	1812:1829	prognostic markers for specific tumors	1812:1849	GENERAL SIGNIFICANCE Methylation status in promoters of candidate glyco-genes might serve as prognostic markers for specific tumors and point to potential novel targets for epigenetic drugs.
26794090	11	48	theme	GENERAL	1719:1725	arg1	status					1752:1757	GENERAL SIGNIFICANCE Methylation status	1719:1757	GENERAL SIGNIFICANCE Methylation status in promoters of candidate glyco-genes	1719:1795	GENERAL SIGNIFICANCE Methylation status in promoters of candidate glyco-genes might serve as prognostic markers for specific tumors and point to potential novel targets for epigenetic drugs.
26794090	0	49	theme	aberrant	48:55	arg1	methylation					57:67	aberrant methylation	48:67	aberrant methylation	48:67	Glyco-genes change expression in cancer through aberrant methylation.
26794090	6	50	theme	tumors	1125:1130	arg1	type					1117:1120	the same type	1108:1120	the same type of tumors	1108:1130	RESULTS Ten glyco-genes (GALNT3, GALNT6, GALNT7, GALNT14, MGAT3, MAN1A1, MAN1C1, ST3GAL2, ST6GAL1, ST8SIA3) showing changes in both methylation and expression in the same type of cancer belong to GalNAc transferases, GlcNAc transferases, mannosidases and sialyltransferases, which is in line with changes in glycan structures already reported in the same type of tumors.
26794090	6	51	theme	cancer	941:946	arg1	type					933:936	the same type	924:936	the same type of cancer	924:946	RESULTS Ten glyco-genes (GALNT3, GALNT6, GALNT7, GALNT14, MGAT3, MAN1A1, MAN1C1, ST3GAL2, ST6GAL1, ST8SIA3) showing changes in both methylation and expression in the same type of cancer belong to GalNAc transferases, GlcNAc transferases, mannosidases and sialyltransferases, which is in line with changes in glycan structures already reported in the same type of tumors.
26794090	10	52	theme	CONCLUSIONS	1541:1551	arg1	expression					1562:1571	CONCLUSIONS Aberrant expression	1541:1571	CONCLUSIONS Aberrant expression of glyco-genes due to aberrant promoter methylation	1541:1623	CONCLUSIONS Aberrant expression of glyco-genes due to aberrant promoter methylation could be a way leading to characteristic glycosylation profiles commonly described in cancer.
26794090	10	52	theme	CONCLUSIONS	1541:1551	arg1	way					1636:1638	a way	1634:1638	a way leading to characteristic glycosylation profiles commonly described in cancer	1634:1716	CONCLUSIONS Aberrant expression of glyco-genes due to aberrant promoter methylation could be a way leading to characteristic glycosylation profiles commonly described in cancer.
26794090	4	53	theme	available	558:566	arg1	databases					568:576	publicly available databases	549:576	publicly available databases	549:576	METHODS We analyzed DNA methylation and expression of 86 glyco-genes in melanoma, hepatocellular, breast and cervical cancers using data from publicly available databases.
26794090	9	54	from	cancer	1500:1505	arg1	expression					1459:1468	aberrant expression	1450:1468	aberrant expression of the GALNT and MAN genes in cancer by aberrant promoter methylation	1450:1538	We also report for the first time aberrant expression of the GALNT and MAN genes in cancer by aberrant promoter methylation.
26794090	4	55	from	databases	568:576	arg1	data					539:542	data	539:542	data from publicly available databases	539:576	METHODS We analyzed DNA methylation and expression of 86 glyco-genes in melanoma, hepatocellular, breast and cervical cancers using data from publicly available databases.
26794090	4	56	from	glyco-genes	464:474	arg1	hepatocellular					489:502	hepatocellular	489:502	hepatocellular	489:502	METHODS We analyzed DNA methylation and expression of 86 glyco-genes in melanoma, hepatocellular, breast and cervical cancers using data from publicly available databases.
26794090	4	56	from	glyco-genes	464:474	arg1	melanoma					479:486	melanoma	479:486	melanoma	479:486	METHODS We analyzed DNA methylation and expression of 86 glyco-genes in melanoma, hepatocellular, breast and cervical cancers using data from publicly available databases.
26794090	4	56	from	glyco-genes	464:474	arg1	breast					505:510	breast	505:510	breast	505:510	METHODS We analyzed DNA methylation and expression of 86 glyco-genes in melanoma, hepatocellular, breast and cervical cancers using data from publicly available databases.
26794090	4	56	from	glyco-genes	464:474	arg1	cancers					525:531	cervical cancers	516:531	cervical cancers	516:531	METHODS We analyzed DNA methylation and expression of 86 glyco-genes in melanoma, hepatocellular, breast and cervical cancers using data from publicly available databases.
26794090	10	57	theme	glyco-genes	1576:1586	arg1	expression					1562:1571	CONCLUSIONS Aberrant expression	1541:1571	CONCLUSIONS Aberrant expression of glyco-genes due to aberrant promoter methylation	1541:1623	CONCLUSIONS Aberrant expression of glyco-genes due to aberrant promoter methylation could be a way leading to characteristic glycosylation profiles commonly described in cancer.
26794090	10	57	theme	glyco-genes	1576:1586	arg1	way					1636:1638	a way	1634:1638	a way leading to characteristic glycosylation profiles commonly described in cancer	1634:1716	CONCLUSIONS Aberrant expression of glyco-genes due to aberrant promoter methylation could be a way leading to characteristic glycosylation profiles commonly described in cancer.
26794090	9	58	theme	first	1439:1443	arg1	time					1445:1448	the first time	1435:1448	the first time	1435:1448	We also report for the first time aberrant expression of the GALNT and MAN genes in cancer by aberrant promoter methylation.
26794090	9	59	theme	aberrant	1450:1457	arg1	expression					1459:1468	aberrant expression	1450:1468	aberrant expression of the GALNT and MAN genes in cancer by aberrant promoter methylation	1450:1538	We also report for the first time aberrant expression of the GALNT and MAN genes in cancer by aberrant promoter methylation.
26794090	10	60	theme	promoter	1604:1611	arg1	methylation					1613:1623	aberrant promoter methylation	1595:1623	aberrant promoter methylation	1595:1623	CONCLUSIONS Aberrant expression of glyco-genes due to aberrant promoter methylation could be a way leading to characteristic glycosylation profiles commonly described in cancer.
26794090	4	61	from	cancers	525:531	arg1	expression					447:456	expression	447:456	expression	447:456	METHODS We analyzed DNA methylation and expression of 86 glyco-genes in melanoma, hepatocellular, breast and cervical cancers using data from publicly available databases.
26794090	4	61	from	cancers	525:531	arg1	methylation					431:441	DNA methylation	427:441	DNA methylation	427:441	METHODS We analyzed DNA methylation and expression of 86 glyco-genes in melanoma, hepatocellular, breast and cervical cancers using data from publicly available databases.
26794090	6	62	theme	GlcNAc	979:984	arg1	transferases					986:997	GlcNAc transferases	979:997	GlcNAc transferases	979:997	RESULTS Ten glyco-genes (GALNT3, GALNT6, GALNT7, GALNT14, MGAT3, MAN1A1, MAN1C1, ST3GAL2, ST6GAL1, ST8SIA3) showing changes in both methylation and expression in the same type of cancer belong to GalNAc transferases, GlcNAc transferases, mannosidases and sialyltransferases, which is in line with changes in glycan structures already reported in the same type of tumors.
26794090	9	63	theme	GALNT	1477:1481	arg1	genes					1491:1495	the GALNT and MAN genes	1473:1495	the GALNT and MAN genes in cancer	1473:1505	We also report for the first time aberrant expression of the GALNT and MAN genes in cancer by aberrant promoter methylation.
26794090	12	64	theme	personalised	1971:1982	arg1	medicine					1984:1991	personalised medicine	1971:1991	personalised medicine" Guest Editor	1971:2005	This article is part of a Special Issue entitled "Glycans in personalised medicine" Guest Editor: Professor Gordan Lauc.
26794090	5	65	theme	brain	744:748	arg1	metastases					750:759	lymph node and brain metastases	729:759	lymph node and brain metastases	729:759	We also analyzed methylation datasets without the available matching expression data for glyco-genes in lung cancer, and progression of melanoma into lymph node and brain metastases.
26794090	2	66	theme	malignant	253:261	arg1	transformation					263:276	malignant transformation	253:276	malignant transformation	253:276	Aberrant glycosylation is profoundly involved in malignant transformation, tumor progression and metastasis.
26794090	9	67	theme	MAN	1487:1489	arg1	genes					1491:1495	the GALNT and MAN genes	1473:1495	the GALNT and MAN genes in cancer	1473:1505	We also report for the first time aberrant expression of the GALNT and MAN genes in cancer by aberrant promoter methylation.
26794090	11	68	theme	potential	1864:1872	arg1	targets					1880:1886	potential novel targets	1864:1886	potential novel targets for epigenetic drugs	1864:1907	GENERAL SIGNIFICANCE Methylation status in promoters of candidate glyco-genes might serve as prognostic markers for specific tumors and point to potential novel targets for epigenetic drugs.
26794090	5	69	theme	matching	639:646	arg1	data					659:662	the available matching expression data	625:662	the available matching expression data for glyco-genes in lung cancer	625:693	We also analyzed methylation datasets without the available matching expression data for glyco-genes in lung cancer, and progression of melanoma into lymph node and brain metastases.
26794090	1	70	mod	modified	110:117	arg3	addition					131:138	covalent addition	122:138	covalent addition of glycan molecules that considerably influence their function	122:201	BACKGROUND Most eukaryotic proteins are modified by covalent addition of glycan molecules that considerably influence their function.
26794090	1	70	mod	modified	110:117	arg1	proteins					97:104	BACKGROUND Most eukaryotic proteins	70:104	BACKGROUND Most eukaryotic proteins	70:104	BACKGROUND Most eukaryotic proteins are modified by covalent addition of glycan molecules that considerably influence their function.
26794090	2	71	gly	glycosylation	213:225	arg1	transformation					263:276	malignant transformation	253:276	malignant transformation	253:276	Aberrant glycosylation is profoundly involved in malignant transformation, tumor progression and metastasis.
26794090	2	71	gly	glycosylation	213:225	arg1	progression					285:295	tumor progression	279:295	tumor progression	279:295	Aberrant glycosylation is profoundly involved in malignant transformation, tumor progression and metastasis.
26794090	2	71	gly	glycosylation	213:225	arg1	metastasis					301:310	metastasis	301:310	metastasis	301:310	Aberrant glycosylation is profoundly involved in malignant transformation, tumor progression and metastasis.
26794090	1	72	theme	molecules	150:158	arg1	addition					131:138	covalent addition	122:138	covalent addition of glycan molecules that considerably influence their function	122:201	BACKGROUND Most eukaryotic proteins are modified by covalent addition of glycan molecules that considerably influence their function.
26794090	8	73	theme	novel	1313:1317	arg1	gene					1329:1332	a novel candidate gene	1311:1332	a novel candidate gene that is epigenetically dysregulated in different cancers other than brain cancer	1311:1413	The MGAT5B gene, so far identified as specifically expressed in brain, emerged as a novel candidate gene that is epigenetically dysregulated in different cancers other than brain cancer.
26794090	11	74	theme	glyco-genes	1785:1795	arg1	promoters					1762:1770	promoters	1762:1770	promoters of candidate glyco-genes	1762:1795	GENERAL SIGNIFICANCE Methylation status in promoters of candidate glyco-genes might serve as prognostic markers for specific tumors and point to potential novel targets for epigenetic drugs.
26794090	9	75	theme	promoter	1519:1526	arg1	methylation					1528:1538	aberrant promoter methylation	1510:1538	aberrant promoter methylation	1510:1538	We also report for the first time aberrant expression of the GALNT and MAN genes in cancer by aberrant promoter methylation.
26794090	3	76	theme	glycan	377:382	arg1	pathways					397:404	disturbed glycan biosynthesis pathways	367:404	disturbed glycan biosynthesis pathways	367:404	Some glycan structures are tumor-specific and reflect disturbed glycan biosynthesis pathways.
26794090	5	77	theme	lung	683:686	arg1	cancer					688:693	lung cancer	683:693	lung cancer	683:693	We also analyzed methylation datasets without the available matching expression data for glyco-genes in lung cancer, and progression of melanoma into lymph node and brain metastases.
26794090	6	78	dep	RESULTS	762:768	arg1	glyco-genes					774:784	Ten glyco-genes	770:784	RESULTS Ten glyco-genes (GALNT3, GALNT6, GALNT7, GALNT14, MGAT3, MAN1A1, MAN1C1, ST3GAL2, ST6GAL1, ST8SIA3) showing changes in both methylation and expression in the same type of cancer	762:946	RESULTS Ten glyco-genes (GALNT3, GALNT6, GALNT7, GALNT14, MGAT3, MAN1A1, MAN1C1, ST3GAL2, ST6GAL1, ST8SIA3) showing changes in both methylation and expression in the same type of cancer belong to GalNAc transferases, GlcNAc transferases, mannosidases and sialyltransferases, which is in line with changes in glycan structures already reported in the same type of tumors.
26794090	11	79	theme	prognostic	1812:1821	arg1	markers					1823:1829	prognostic markers	1812:1829	prognostic markers for specific tumors	1812:1849	GENERAL SIGNIFICANCE Methylation status in promoters of candidate glyco-genes might serve as prognostic markers for specific tumors and point to potential novel targets for epigenetic drugs.
26794090	11	79	theme	prognostic	1812:1821	arg1	status					1752:1757	GENERAL SIGNIFICANCE Methylation status	1719:1757	GENERAL SIGNIFICANCE Methylation status in promoters of candidate glyco-genes	1719:1795	GENERAL SIGNIFICANCE Methylation status in promoters of candidate glyco-genes might serve as prognostic markers for specific tumors and point to potential novel targets for epigenetic drugs.
26794090	1	80	theme	Most	81:84	arg1	proteins					97:104	BACKGROUND Most eukaryotic proteins	70:104	BACKGROUND Most eukaryotic proteins	70:104	BACKGROUND Most eukaryotic proteins are modified by covalent addition of glycan molecules that considerably influence their function.
26794090	6	81	dep	glyco-genes	774:784	arg1	MAN1C1					835:840	MAN1C1	835:840	MAN1C1	835:840	RESULTS Ten glyco-genes (GALNT3, GALNT6, GALNT7, GALNT14, MGAT3, MAN1A1, MAN1C1, ST3GAL2, ST6GAL1, ST8SIA3) showing changes in both methylation and expression in the same type of cancer belong to GalNAc transferases, GlcNAc transferases, mannosidases and sialyltransferases, which is in line with changes in glycan structures already reported in the same type of tumors.
26794090	6	81	dep	glyco-genes	774:784	arg1	ST8SIA3					861:867	ST8SIA3	861:867	ST8SIA3	861:867	RESULTS Ten glyco-genes (GALNT3, GALNT6, GALNT7, GALNT14, MGAT3, MAN1A1, MAN1C1, ST3GAL2, ST6GAL1, ST8SIA3) showing changes in both methylation and expression in the same type of cancer belong to GalNAc transferases, GlcNAc transferases, mannosidases and sialyltransferases, which is in line with changes in glycan structures already reported in the same type of tumors.
26794090	6	81	dep	glyco-genes	774:784	arg1	GALNT3					787:792	GALNT3	787:792	GALNT3	787:792	RESULTS Ten glyco-genes (GALNT3, GALNT6, GALNT7, GALNT14, MGAT3, MAN1A1, MAN1C1, ST3GAL2, ST6GAL1, ST8SIA3) showing changes in both methylation and expression in the same type of cancer belong to GalNAc transferases, GlcNAc transferases, mannosidases and sialyltransferases, which is in line with changes in glycan structures already reported in the same type of tumors.
26794090	6	81	dep	glyco-genes	774:784	arg1	GALNT14					811:817	GALNT14	811:817	GALNT14	811:817	RESULTS Ten glyco-genes (GALNT3, GALNT6, GALNT7, GALNT14, MGAT3, MAN1A1, MAN1C1, ST3GAL2, ST6GAL1, ST8SIA3) showing changes in both methylation and expression in the same type of cancer belong to GalNAc transferases, GlcNAc transferases, mannosidases and sialyltransferases, which is in line with changes in glycan structures already reported in the same type of tumors.
26794090	6	81	dep	glyco-genes	774:784	arg1	MGAT3					820:824	MGAT3	820:824	MGAT3	820:824	RESULTS Ten glyco-genes (GALNT3, GALNT6, GALNT7, GALNT14, MGAT3, MAN1A1, MAN1C1, ST3GAL2, ST6GAL1, ST8SIA3) showing changes in both methylation and expression in the same type of cancer belong to GalNAc transferases, GlcNAc transferases, mannosidases and sialyltransferases, which is in line with changes in glycan structures already reported in the same type of tumors.
26794090	6	81	dep	glyco-genes	774:784	arg1	ST3GAL2					843:849	ST3GAL2	843:849	ST3GAL2	843:849	RESULTS Ten glyco-genes (GALNT3, GALNT6, GALNT7, GALNT14, MGAT3, MAN1A1, MAN1C1, ST3GAL2, ST6GAL1, ST8SIA3) showing changes in both methylation and expression in the same type of cancer belong to GalNAc transferases, GlcNAc transferases, mannosidases and sialyltransferases, which is in line with changes in glycan structures already reported in the same type of tumors.
26794090	6	81	dep	glyco-genes	774:784	arg1	GALNT6					795:800	GALNT6	795:800	GALNT6	795:800	RESULTS Ten glyco-genes (GALNT3, GALNT6, GALNT7, GALNT14, MGAT3, MAN1A1, MAN1C1, ST3GAL2, ST6GAL1, ST8SIA3) showing changes in both methylation and expression in the same type of cancer belong to GalNAc transferases, GlcNAc transferases, mannosidases and sialyltransferases, which is in line with changes in glycan structures already reported in the same type of tumors.
26794090	6	81	dep	glyco-genes	774:784	arg1	ST6GAL1					852:858	ST6GAL1	852:858	ST6GAL1	852:858	RESULTS Ten glyco-genes (GALNT3, GALNT6, GALNT7, GALNT14, MGAT3, MAN1A1, MAN1C1, ST3GAL2, ST6GAL1, ST8SIA3) showing changes in both methylation and expression in the same type of cancer belong to GalNAc transferases, GlcNAc transferases, mannosidases and sialyltransferases, which is in line with changes in glycan structures already reported in the same type of tumors.
26794090	6	81	dep	glyco-genes	774:784	arg1	GALNT7					803:808	GALNT7	803:808	GALNT7	803:808	RESULTS Ten glyco-genes (GALNT3, GALNT6, GALNT7, GALNT14, MGAT3, MAN1A1, MAN1C1, ST3GAL2, ST6GAL1, ST8SIA3) showing changes in both methylation and expression in the same type of cancer belong to GalNAc transferases, GlcNAc transferases, mannosidases and sialyltransferases, which is in line with changes in glycan structures already reported in the same type of tumors.
26794090	6	81	dep	glyco-genes	774:784	arg1	MAN1A1					827:832	MAN1A1	827:832	MAN1A1	827:832	RESULTS Ten glyco-genes (GALNT3, GALNT6, GALNT7, GALNT14, MGAT3, MAN1A1, MAN1C1, ST3GAL2, ST6GAL1, ST8SIA3) showing changes in both methylation and expression in the same type of cancer belong to GalNAc transferases, GlcNAc transferases, mannosidases and sialyltransferases, which is in line with changes in glycan structures already reported in the same type of tumors.
26794090	10	82	theme	glycosylation	1666:1678	arg1	profiles					1680:1687	characteristic glycosylation profiles	1651:1687	characteristic glycosylation profiles commonly described in cancer	1651:1716	CONCLUSIONS Aberrant expression of glyco-genes due to aberrant promoter methylation could be a way leading to characteristic glycosylation profiles commonly described in cancer.
26794090	12	83	theme	Issue	1944:1948	arg1	part					1926:1929	part	1926:1929	part of a Special Issue entitled "Glycans in personalised medicine" Guest Editor: Professor Gordan Lauc	1926:2028	This article is part of a Special Issue entitled "Glycans in personalised medicine" Guest Editor: Professor Gordan Lauc.
26794090	8	84	theme	different	1373:1381	arg1	cancers					1383:1389	different cancers	1373:1389	different cancers other than brain cancer	1373:1413	The MGAT5B gene, so far identified as specifically expressed in brain, emerged as a novel candidate gene that is epigenetically dysregulated in different cancers other than brain cancer.
26794090	6	85	theme	glycan	1070:1075	arg1	structures					1077:1086	glycan structures	1070:1086	glycan structures already reported in the same type of tumors	1070:1130	RESULTS Ten glyco-genes (GALNT3, GALNT6, GALNT7, GALNT14, MGAT3, MAN1A1, MAN1C1, ST3GAL2, ST6GAL1, ST8SIA3) showing changes in both methylation and expression in the same type of cancer belong to GalNAc transferases, GlcNAc transferases, mannosidases and sialyltransferases, which is in line with changes in glycan structures already reported in the same type of tumors.
26794090	11	86	theme	SIGNIFICANCE	1727:1738	arg1	markers					1823:1829	prognostic markers	1812:1829	prognostic markers for specific tumors	1812:1849	GENERAL SIGNIFICANCE Methylation status in promoters of candidate glyco-genes might serve as prognostic markers for specific tumors and point to potential novel targets for epigenetic drugs.
26794090	11	86	theme	SIGNIFICANCE	1727:1738	arg1	status					1752:1757	GENERAL SIGNIFICANCE Methylation status	1719:1757	GENERAL SIGNIFICANCE Methylation status in promoters of candidate glyco-genes	1719:1795	GENERAL SIGNIFICANCE Methylation status in promoters of candidate glyco-genes might serve as prognostic markers for specific tumors and point to potential novel targets for epigenetic drugs.
26794090	4	87	dep	METHODS	407:413	arg1	analyzed					418:425	analyzed	418:425	analyzed DNA methylation and expression of 86 glyco-genes in melanoma, hepatocellular, breast and cervical cancers using data from publicly available databases	418:576	METHODS We analyzed DNA methylation and expression of 86 glyco-genes in melanoma, hepatocellular, breast and cervical cancers using data from publicly available databases.
26794090	8	88	theme	brain	1402:1406	arg1	cancer					1408:1413	brain cancer	1402:1413	different cancers other than brain cancer	1373:1413	The MGAT5B gene, so far identified as specifically expressed in brain, emerged as a novel candidate gene that is epigenetically dysregulated in different cancers other than brain cancer.
26794090	5	89	theme	node	735:738	arg1	metastases					750:759	lymph node and brain metastases	729:759	lymph node and brain metastases	729:759	We also analyzed methylation datasets without the available matching expression data for glyco-genes in lung cancer, and progression of melanoma into lymph node and brain metastases.
26794090	11	90	theme	epigenetic	1892:1901	arg1	drugs					1903:1907	epigenetic drugs	1892:1907	epigenetic drugs	1892:1907	GENERAL SIGNIFICANCE Methylation status in promoters of candidate glyco-genes might serve as prognostic markers for specific tumors and point to potential novel targets for epigenetic drugs.
26794090	9	91	from	expression	1459:1468	arg1	cancer					1500:1505	cancer	1500:1505	cancer	1500:1505	We also report for the first time aberrant expression of the GALNT and MAN genes in cancer by aberrant promoter methylation.
26794090	10	92	theme	Aberrant	1553:1560	arg1	expression					1562:1571	CONCLUSIONS Aberrant expression	1541:1571	CONCLUSIONS Aberrant expression of glyco-genes due to aberrant promoter methylation	1541:1623	CONCLUSIONS Aberrant expression of glyco-genes due to aberrant promoter methylation could be a way leading to characteristic glycosylation profiles commonly described in cancer.
26794090	10	92	theme	Aberrant	1553:1560	arg1	way					1636:1638	a way	1634:1638	a way leading to characteristic glycosylation profiles commonly described in cancer	1634:1716	CONCLUSIONS Aberrant expression of glyco-genes due to aberrant promoter methylation could be a way leading to characteristic glycosylation profiles commonly described in cancer.
24909906	3	0	theme	true	465:468	arg1	method					441:446	the one-step/two-step method	419:446	the one-step/two-step method	419:446	Despite these differences, the one-step/two-step method can be seen as a true alternative to the more labor-intensive Updegraff method.
24909906	3	0	theme	true	465:468	arg1	alternative					470:480	a true alternative	463:480	a true alternative to the more labor-intensive Updegraff method	463:525	Despite these differences, the one-step/two-step method can be seen as a true alternative to the more labor-intensive Updegraff method.
24909906	1	1	theme	one-step/two-step	109:125	arg1	method					138:143	a one-step/two-step hydrolysis method	107:143	a one-step/two-step hydrolysis method	107:143	The cellulose analysis results of four feedstocks and Avicel obtained by a one-step/two-step hydrolysis method were compared to the conventional cellulose assay according to Updegraff.
24909906	1	2	theme	conventional	166:177	arg1	assay					189:193	the conventional cellulose assay	162:193	the conventional cellulose assay	162:193	The cellulose analysis results of four feedstocks and Avicel obtained by a one-step/two-step hydrolysis method were compared to the conventional cellulose assay according to Updegraff.
24909906	2	3	theme	corn	283:286	arg1	stover					288:293	corn stover	283:293	corn stover (97%)	283:299	Slightly lower cellulose levels were observed for Avicel (97%), corn stover (97%), poplar (96%), and Miscanthus (94%) but for pine the amounts were almost identical (101%).
24909906	2	3	theme	corn	283:286	arg1	%					298:298	97%	296:298	97%	296:298	Slightly lower cellulose levels were observed for Avicel (97%), corn stover (97%), poplar (96%), and Miscanthus (94%) but for pine the amounts were almost identical (101%).
24909906	3	4	theme	Updegraff	510:518	arg1	method					520:525	the more labor-intensive Updegraff method	485:525	the more labor-intensive Updegraff method	485:525	Despite these differences, the one-step/two-step method can be seen as a true alternative to the more labor-intensive Updegraff method.
24909906	1	5	theme	feedstocks	73:82	arg1	results					57:63	The cellulose analysis results	34:63	The cellulose analysis results of four feedstocks and Avicel obtained by a one-step/two-step hydrolysis method	34:143	The cellulose analysis results of four feedstocks and Avicel obtained by a one-step/two-step hydrolysis method were compared to the conventional cellulose assay according to Updegraff.
24909906	1	6	theme	hydrolysis	127:136	arg1	method					138:143	a one-step/two-step hydrolysis method	107:143	a one-step/two-step hydrolysis method	107:143	The cellulose analysis results of four feedstocks and Avicel obtained by a one-step/two-step hydrolysis method were compared to the conventional cellulose assay according to Updegraff.
24909906	1	7	theme	cellulose	179:187	arg1	assay					189:193	the conventional cellulose assay	162:193	the conventional cellulose assay	162:193	The cellulose analysis results of four feedstocks and Avicel obtained by a one-step/two-step hydrolysis method were compared to the conventional cellulose assay according to Updegraff.
24909906	3	8	theme	one-step/two-step	423:439	arg1	method					441:446	the one-step/two-step method	419:446	the one-step/two-step method	419:446	Despite these differences, the one-step/two-step method can be seen as a true alternative to the more labor-intensive Updegraff method.
24909906	3	8	theme	one-step/two-step	423:439	arg1	alternative					470:480	a true alternative	463:480	a true alternative to the more labor-intensive Updegraff method	463:525	Despite these differences, the one-step/two-step method can be seen as a true alternative to the more labor-intensive Updegraff method.
24909906	2	9	theme	cellulose	234:242	arg1	levels					244:249	Slightly lower cellulose levels	219:249	Slightly lower cellulose levels	219:249	Slightly lower cellulose levels were observed for Avicel (97%), corn stover (97%), poplar (96%), and Miscanthus (94%) but for pine the amounts were almost identical (101%).
24909906	0	10	theme	Rapid	0:4	arg1	determination					6:18	Rapid determination	0:18	Rapid determination of cellulose.	0:32	Rapid determination of cellulose.
24909906	1	11	theme	cellulose	38:46	arg1	results					57:63	The cellulose analysis results	34:63	The cellulose analysis results of four feedstocks and Avicel obtained by a one-step/two-step hydrolysis method	34:143	The cellulose analysis results of four feedstocks and Avicel obtained by a one-step/two-step hydrolysis method were compared to the conventional cellulose assay according to Updegraff.
24909906	1	12	theme	Avicel	88:93	arg1	results					57:63	The cellulose analysis results	34:63	The cellulose analysis results of four feedstocks and Avicel obtained by a one-step/two-step hydrolysis method	34:143	The cellulose analysis results of four feedstocks and Avicel obtained by a one-step/two-step hydrolysis method were compared to the conventional cellulose assay according to Updegraff.
24909906	3	13	theme	labor-intensive	494:508	arg1	method					520:525	the more labor-intensive Updegraff method	485:525	the more labor-intensive Updegraff method	485:525	Despite these differences, the one-step/two-step method can be seen as a true alternative to the more labor-intensive Updegraff method.
24909906	2	14	dep	identical	374:382	arg1	amounts					354:360	the amounts	350:360	the amounts	350:360	Slightly lower cellulose levels were observed for Avicel (97%), corn stover (97%), poplar (96%), and Miscanthus (94%) but for pine the amounts were almost identical (101%).
24909906	2	14	dep	identical	374:382	arg1	identical					374:382	identical	374:382	identical	374:382	Slightly lower cellulose levels were observed for Avicel (97%), corn stover (97%), poplar (96%), and Miscanthus (94%) but for pine the amounts were almost identical (101%).
24909906	2	14	dep	identical	374:382	arg1	%					388:388	101%	385:388	101%	385:388	Slightly lower cellulose levels were observed for Avicel (97%), corn stover (97%), poplar (96%), and Miscanthus (94%) but for pine the amounts were almost identical (101%).
24909906	0	15	theme	cellulose	23:31	arg1	determination					6:18	Rapid determination	0:18	Rapid determination of cellulose.	0:32	Rapid determination of cellulose.
24909906	1	16	theme	analysis	48:55	arg1	results					57:63	The cellulose analysis results	34:63	The cellulose analysis results of four feedstocks and Avicel obtained by a one-step/two-step hydrolysis method	34:143	The cellulose analysis results of four feedstocks and Avicel obtained by a one-step/two-step hydrolysis method were compared to the conventional cellulose assay according to Updegraff.
24909906	2	17	theme	lower	228:232	arg1	levels					244:249	Slightly lower cellulose levels	219:249	Slightly lower cellulose levels	219:249	Slightly lower cellulose levels were observed for Avicel (97%), corn stover (97%), poplar (96%), and Miscanthus (94%) but for pine the amounts were almost identical (101%).
25263900	2	0	with	reduction	401:409	arg1	dithiothreitol					433:446	dithiothreitol	433:446	dithiothreitol	433:446	Thiolation was achieved by the use of the disulfide containing crosslinker 3,3'-dithiobis(propanoic hydrazide) and subsequent reduction of the disulfide with dithiothreitol.
25263900	8	1	theme	contact	1007:1013	arg1	measurement					1021:1031	water contact angle measurement	1001:1031	water contact angle measurement (WCA)	1001:1037	The results of water contact angle measurement (WCA), ellipsometry and confocal laser scanning microscopy (CLSM) demonstrated that the resulting surface coverage was dependent on the degree of thiolation of GAGs.
25263900	8	1	theme	contact	1007:1013	arg1	WCA					1034:1036	WCA	1034:1036	WCA	1034:1036	The results of water contact angle measurement (WCA), ellipsometry and confocal laser scanning microscopy (CLSM) demonstrated that the resulting surface coverage was dependent on the degree of thiolation of GAGs.
25263900	5	2	theme	thiol	739:743	arg1	concentration					745:757	the thiol concentration	735:757	the thiol concentration of tGAGs	735:766	Ellman's reagent was used to quantify the thiol concentration of tGAGs.
25263900	8	3	theme	ellipsometry	1040:1051	arg1	results					990:996	The results	986:996	The results of water contact angle measurement (WCA), ellipsometry and confocal laser scanning microscopy (CLSM)	986:1097	The results of water contact angle measurement (WCA), ellipsometry and confocal laser scanning microscopy (CLSM) demonstrated that the resulting surface coverage was dependent on the degree of thiolation of GAGs.
25263900	7	4	theme	UV	976:977	arg1	light					979:983	UV light	976:983	UV light	976:983	This was achieved by homogeneous immobilization from solution as well as with microcontact printing and exposure to UV light.
25263900	8	5	theme	thiolation	1179:1188	arg1	degree					1169:1174	the degree	1165:1174	the degree of thiolation of GAGs	1165:1196	The results of water contact angle measurement (WCA), ellipsometry and confocal laser scanning microscopy (CLSM) demonstrated that the resulting surface coverage was dependent on the degree of thiolation of GAGs.
25263900	8	6	theme	laser	1066:1070	arg1	CLSM					1093:1096	CLSM	1093:1096	CLSM	1093:1096	The results of water contact angle measurement (WCA), ellipsometry and confocal laser scanning microscopy (CLSM) demonstrated that the resulting surface coverage was dependent on the degree of thiolation of GAGs.
25263900	8	6	theme	laser	1066:1070	arg1	microscopy					1081:1090	confocal laser scanning microscopy	1057:1090	confocal laser scanning microscopy (CLSM)	1057:1097	The results of water contact angle measurement (WCA), ellipsometry and confocal laser scanning microscopy (CLSM) demonstrated that the resulting surface coverage was dependent on the degree of thiolation of GAGs.
25263900	8	7	theme	water	1001:1005	arg1	measurement					1021:1031	water contact angle measurement	1001:1031	water contact angle measurement (WCA)	1001:1037	The results of water contact angle measurement (WCA), ellipsometry and confocal laser scanning microscopy (CLSM) demonstrated that the resulting surface coverage was dependent on the degree of thiolation of GAGs.
25263900	8	7	theme	water	1001:1005	arg1	WCA					1034:1036	WCA	1034:1036	WCA	1034:1036	The results of water contact angle measurement (WCA), ellipsometry and confocal laser scanning microscopy (CLSM) demonstrated that the resulting surface coverage was dependent on the degree of thiolation of GAGs.
25263900	7	8	from	solution	913:920	arg1	immobilization					893:906	homogeneous immobilization	881:906	homogeneous immobilization from solution as well as with microcontact printing and exposure to UV light	881:983	This was achieved by homogeneous immobilization from solution as well as with microcontact printing and exposure to UV light.
25263900	4	9	theme	products	687:694	arg1	structure					649:657	structure	649:657	structure	649:657	The tGAGs were characterized by (1)H-NMR, Raman and flow-field-flow-fractionation (A4F) to determine the chemical composition, structure and molecular weight of the products.
25263900	4	9	theme	products	687:694	arg1	weight					673:678	molecular weight	663:678	molecular weight	663:678	The tGAGs were characterized by (1)H-NMR, Raman and flow-field-flow-fractionation (A4F) to determine the chemical composition, structure and molecular weight of the products.
25263900	4	9	theme	products	687:694	arg1	composition					636:646	the chemical composition	623:646	the chemical composition	623:646	The tGAGs were characterized by (1)H-NMR, Raman and flow-field-flow-fractionation (A4F) to determine the chemical composition, structure and molecular weight of the products.
25263900	1	10	theme	thiolated	168:176	arg1	tGAGs					189:193	tGAGs	189:193	tGAGs	189:193	Glycosaminoglycans (GAGs) with varying degree of sulfation were chemically modified to obtain thiolated analogues (tGAGs) for subsequent surface grafting on vinyl-terminated self-assembled monolayers.
25263900	1	10	theme	thiolated	168:176	arg1	analogues					178:186	thiolated analogues	168:186	thiolated analogues (tGAGs)	168:194	Glycosaminoglycans (GAGs) with varying degree of sulfation were chemically modified to obtain thiolated analogues (tGAGs) for subsequent surface grafting on vinyl-terminated self-assembled monolayers.
25263900	8	11	theme	confocal	1057:1064	arg1	CLSM					1093:1096	CLSM	1093:1096	CLSM	1093:1096	The results of water contact angle measurement (WCA), ellipsometry and confocal laser scanning microscopy (CLSM) demonstrated that the resulting surface coverage was dependent on the degree of thiolation of GAGs.
25263900	8	11	theme	confocal	1057:1064	arg1	microscopy					1081:1090	confocal laser scanning microscopy	1057:1090	confocal laser scanning microscopy (CLSM)	1057:1097	The results of water contact angle measurement (WCA), ellipsometry and confocal laser scanning microscopy (CLSM) demonstrated that the resulting surface coverage was dependent on the degree of thiolation of GAGs.
25263900	6	12	theme	vinyl-terminated	801:816	arg1	glass					818:822	vinyl-terminated glass	801:822	vinyl-terminated glass	801:822	The tGAGs were immobilized onto vinyl-terminated glass and silicon via thiol-ene reaction.
25263900	8	13	theme	GAGs	1193:1196	arg1	thiolation					1179:1188	thiolation	1179:1188	thiolation of GAGs	1179:1196	The results of water contact angle measurement (WCA), ellipsometry and confocal laser scanning microscopy (CLSM) demonstrated that the resulting surface coverage was dependent on the degree of thiolation of GAGs.
25263900	8	14	theme	microscopy	1081:1090	arg1	results					990:996	The results	986:996	The results of water contact angle measurement (WCA), ellipsometry and confocal laser scanning microscopy (CLSM)	986:1097	The results of water contact angle measurement (WCA), ellipsometry and confocal laser scanning microscopy (CLSM) demonstrated that the resulting surface coverage was dependent on the degree of thiolation of GAGs.
25263900	8	15	theme	angle	1015:1019	arg1	measurement					1021:1031	water contact angle measurement	1001:1031	water contact angle measurement (WCA)	1001:1037	The results of water contact angle measurement (WCA), ellipsometry and confocal laser scanning microscopy (CLSM) demonstrated that the resulting surface coverage was dependent on the degree of thiolation of GAGs.
25263900	8	15	theme	angle	1015:1019	arg1	WCA					1034:1036	WCA	1034:1036	WCA	1034:1036	The results of water contact angle measurement (WCA), ellipsometry and confocal laser scanning microscopy (CLSM) demonstrated that the resulting surface coverage was dependent on the degree of thiolation of GAGs.
25263900	3	16	theme	different	453:461	arg1	ratios					469:474	Two different molar ratios	449:474	Two different molar ratios of the crosslinker	449:493	Two different molar ratios of the crosslinker were used for conjugation.
25263900	6	17	theme	thiol-ene	840:848	arg1	reaction					850:857	thiol-ene reaction	840:857	thiol-ene reaction	840:857	The tGAGs were immobilized onto vinyl-terminated glass and silicon via thiol-ene reaction.
25263900	2	18	theme	disulfide	317:325	arg1	use					306:308	the use	302:308	the use of the disulfide containing crosslinker 3,3'-dithiobis(propanoic hydrazide) and subsequent reduction of the disulfide with dithiothreitol	302:446	Thiolation was achieved by the use of the disulfide containing crosslinker 3,3'-dithiobis(propanoic hydrazide) and subsequent reduction of the disulfide with dithiothreitol.
25263900	3	19	theme	molar	463:467	arg1	ratios					469:474	Two different molar ratios	449:474	Two different molar ratios of the crosslinker	449:493	Two different molar ratios of the crosslinker were used for conjugation.
25263900	0	20	theme	glycosaminoglycans	23:40	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of thiolated glycosaminoglycans	0:40	Synthesis of thiolated glycosaminoglycans and grafting to solid surfaces.
25263900	3	21	used	used	500:503	arg2	ratios					469:474	Two different molar ratios	449:474	Two different molar ratios of the crosslinker	449:493	Two different molar ratios of the crosslinker were used for conjugation.
25263900	0	22	theme	thiolated	13:21	arg1	glycosaminoglycans					23:40	thiolated glycosaminoglycans	13:40	thiolated glycosaminoglycans	13:40	Synthesis of thiolated glycosaminoglycans and grafting to solid surfaces.
25263900	2	23	with	3,3'-dithiobis	350:363	arg1	dithiothreitol					433:446	dithiothreitol	433:446	dithiothreitol	433:446	Thiolation was achieved by the use of the disulfide containing crosslinker 3,3'-dithiobis(propanoic hydrazide) and subsequent reduction of the disulfide with dithiothreitol.
25263900	1	24	theme	varying	105:111	arg1	degree					113:118	varying degree	105:118	varying degree of sulfation	105:131	Glycosaminoglycans (GAGs) with varying degree of sulfation were chemically modified to obtain thiolated analogues (tGAGs) for subsequent surface grafting on vinyl-terminated self-assembled monolayers.
25263900	1	25	with	Glycosaminoglycans	74:91	arg1	degree					113:118	varying degree	105:118	varying degree of sulfation	105:131	Glycosaminoglycans (GAGs) with varying degree of sulfation were chemically modified to obtain thiolated analogues (tGAGs) for subsequent surface grafting on vinyl-terminated self-assembled monolayers.
25263900	7	26	theme	homogeneous	881:891	arg1	immobilization					893:906	homogeneous immobilization	881:906	homogeneous immobilization from solution as well as with microcontact printing and exposure to UV light	881:983	This was achieved by homogeneous immobilization from solution as well as with microcontact printing and exposure to UV light.
25263900	4	27	theme	molecular	663:671	arg1	weight					673:678	molecular weight	663:678	molecular weight	663:678	The tGAGs were characterized by (1)H-NMR, Raman and flow-field-flow-fractionation (A4F) to determine the chemical composition, structure and molecular weight of the products.
25263900	1	28	theme	subsequent	200:209	arg1	grafting					219:226	subsequent surface grafting	200:226	subsequent surface grafting on vinyl-terminated self-assembled monolayers	200:272	Glycosaminoglycans (GAGs) with varying degree of sulfation were chemically modified to obtain thiolated analogues (tGAGs) for subsequent surface grafting on vinyl-terminated self-assembled monolayers.
25263900	2	29	theme	disulfide	418:426	arg1	reduction					401:409	subsequent reduction	390:409	subsequent reduction of the disulfide with dithiothreitol	390:446	Thiolation was achieved by the use of the disulfide containing crosslinker 3,3'-dithiobis(propanoic hydrazide) and subsequent reduction of the disulfide with dithiothreitol.
25263900	2	29	theme	disulfide	418:426	arg1	3,3'-dithiobis					350:363	crosslinker 3,3'-dithiobis	338:363	crosslinker 3,3'-dithiobis(propanoic hydrazide)	338:384	Thiolation was achieved by the use of the disulfide containing crosslinker 3,3'-dithiobis(propanoic hydrazide) and subsequent reduction of the disulfide with dithiothreitol.
25263900	2	29	theme	disulfide	418:426	arg1	hydrazide					375:383	propanoic hydrazide	365:383	propanoic hydrazide	365:383	Thiolation was achieved by the use of the disulfide containing crosslinker 3,3'-dithiobis(propanoic hydrazide) and subsequent reduction of the disulfide with dithiothreitol.
25263900	3	30	theme	crosslinker	483:493	arg1	ratios					469:474	Two different molar ratios	449:474	Two different molar ratios of the crosslinker	449:493	Two different molar ratios of the crosslinker were used for conjugation.
25263900	0	31	theme	solid	58:62	arg1	surfaces					64:71	solid surfaces	58:71	solid surfaces	58:71	Synthesis of thiolated glycosaminoglycans and grafting to solid surfaces.
25263900	8	32	theme	surface	1131:1137	arg1	dependent					1152:1160	dependent	1152:1160	dependent	1152:1160	The results of water contact angle measurement (WCA), ellipsometry and confocal laser scanning microscopy (CLSM) demonstrated that the resulting surface coverage was dependent on the degree of thiolation of GAGs.
25263900	8	32	theme	surface	1131:1137	arg1	coverage					1139:1146	the resulting surface coverage	1117:1146	the resulting surface coverage	1117:1146	The results of water contact angle measurement (WCA), ellipsometry and confocal laser scanning microscopy (CLSM) demonstrated that the resulting surface coverage was dependent on the degree of thiolation of GAGs.
25263900	2	33	theme	propanoic	365:373	arg1	3,3'-dithiobis					350:363	crosslinker 3,3'-dithiobis	338:363	crosslinker 3,3'-dithiobis(propanoic hydrazide)	338:384	Thiolation was achieved by the use of the disulfide containing crosslinker 3,3'-dithiobis(propanoic hydrazide) and subsequent reduction of the disulfide with dithiothreitol.
25263900	2	33	theme	propanoic	365:373	arg1	hydrazide					375:383	propanoic hydrazide	365:383	propanoic hydrazide	365:383	Thiolation was achieved by the use of the disulfide containing crosslinker 3,3'-dithiobis(propanoic hydrazide) and subsequent reduction of the disulfide with dithiothreitol.
25263900	7	34	with	immobilization	893:906	arg1	exposure					964:971	exposure	964:971	exposure to UV light	964:983	This was achieved by homogeneous immobilization from solution as well as with microcontact printing and exposure to UV light.
25263900	7	34	with	immobilization	893:906	arg1	printing					951:958	microcontact printing	938:958	microcontact printing	938:958	This was achieved by homogeneous immobilization from solution as well as with microcontact printing and exposure to UV light.
25263900	8	35	theme	scanning	1072:1079	arg1	CLSM					1093:1096	CLSM	1093:1096	CLSM	1093:1096	The results of water contact angle measurement (WCA), ellipsometry and confocal laser scanning microscopy (CLSM) demonstrated that the resulting surface coverage was dependent on the degree of thiolation of GAGs.
25263900	8	35	theme	scanning	1072:1079	arg1	microscopy					1081:1090	confocal laser scanning microscopy	1057:1090	confocal laser scanning microscopy (CLSM)	1057:1097	The results of water contact angle measurement (WCA), ellipsometry and confocal laser scanning microscopy (CLSM) demonstrated that the resulting surface coverage was dependent on the degree of thiolation of GAGs.
25263900	2	36	contain	containing	327:336	arg2	reduction					401:409	subsequent reduction	390:409	subsequent reduction of the disulfide with dithiothreitol	390:446	Thiolation was achieved by the use of the disulfide containing crosslinker 3,3'-dithiobis(propanoic hydrazide) and subsequent reduction of the disulfide with dithiothreitol.
25263900	2	36	contain	containing	327:336	arg1	disulfide					317:325	the disulfide	313:325	the disulfide containing crosslinker 3,3'-dithiobis(propanoic hydrazide) and subsequent reduction of the disulfide with dithiothreitol	313:446	Thiolation was achieved by the use of the disulfide containing crosslinker 3,3'-dithiobis(propanoic hydrazide) and subsequent reduction of the disulfide with dithiothreitol.
25263900	2	36	contain	containing	327:336	arg2	hydrazide					375:383	propanoic hydrazide	365:383	propanoic hydrazide	365:383	Thiolation was achieved by the use of the disulfide containing crosslinker 3,3'-dithiobis(propanoic hydrazide) and subsequent reduction of the disulfide with dithiothreitol.
25263900	2	36	contain	containing	327:336	arg2	3,3'-dithiobis					350:363	crosslinker 3,3'-dithiobis	338:363	crosslinker 3,3'-dithiobis(propanoic hydrazide)	338:384	Thiolation was achieved by the use of the disulfide containing crosslinker 3,3'-dithiobis(propanoic hydrazide) and subsequent reduction of the disulfide with dithiothreitol.
25263900	1	37	from	grafting	219:226	arg1	monolayers					263:272	vinyl-terminated self-assembled monolayers	231:272	vinyl-terminated self-assembled monolayers	231:272	Glycosaminoglycans (GAGs) with varying degree of sulfation were chemically modified to obtain thiolated analogues (tGAGs) for subsequent surface grafting on vinyl-terminated self-assembled monolayers.
25263900	8	38	theme	measurement	1021:1031	arg1	results					990:996	The results	986:996	The results of water contact angle measurement (WCA), ellipsometry and confocal laser scanning microscopy (CLSM)	986:1097	The results of water contact angle measurement (WCA), ellipsometry and confocal laser scanning microscopy (CLSM) demonstrated that the resulting surface coverage was dependent on the degree of thiolation of GAGs.
25263900	1	39	theme	sulfation	123:131	arg1	degree					113:118	varying degree	105:118	varying degree of sulfation	105:131	Glycosaminoglycans (GAGs) with varying degree of sulfation were chemically modified to obtain thiolated analogues (tGAGs) for subsequent surface grafting on vinyl-terminated self-assembled monolayers.
25263900	7	40	theme	microcontact	938:949	arg1	printing					951:958	microcontact printing	938:958	microcontact printing	938:958	This was achieved by homogeneous immobilization from solution as well as with microcontact printing and exposure to UV light.
25263900	8	41	theme	resulting	1121:1129	arg1	dependent					1152:1160	dependent	1152:1160	dependent	1152:1160	The results of water contact angle measurement (WCA), ellipsometry and confocal laser scanning microscopy (CLSM) demonstrated that the resulting surface coverage was dependent on the degree of thiolation of GAGs.
25263900	8	41	theme	resulting	1121:1129	arg1	coverage					1139:1146	the resulting surface coverage	1117:1146	the resulting surface coverage	1117:1146	The results of water contact angle measurement (WCA), ellipsometry and confocal laser scanning microscopy (CLSM) demonstrated that the resulting surface coverage was dependent on the degree of thiolation of GAGs.
25263900	2	42	theme	crosslinker	338:348	arg1	3,3'-dithiobis					350:363	crosslinker 3,3'-dithiobis	338:363	crosslinker 3,3'-dithiobis(propanoic hydrazide)	338:384	Thiolation was achieved by the use of the disulfide containing crosslinker 3,3'-dithiobis(propanoic hydrazide) and subsequent reduction of the disulfide with dithiothreitol.
25263900	2	42	theme	crosslinker	338:348	arg1	hydrazide					375:383	propanoic hydrazide	365:383	propanoic hydrazide	365:383	Thiolation was achieved by the use of the disulfide containing crosslinker 3,3'-dithiobis(propanoic hydrazide) and subsequent reduction of the disulfide with dithiothreitol.
25263900	5	43	theme	tGAGs	762:766	arg1	concentration					745:757	the thiol concentration	735:757	the thiol concentration of tGAGs	735:766	Ellman's reagent was used to quantify the thiol concentration of tGAGs.
25263900	1	44	theme	surface	211:217	arg1	grafting					219:226	subsequent surface grafting	200:226	subsequent surface grafting on vinyl-terminated self-assembled monolayers	200:272	Glycosaminoglycans (GAGs) with varying degree of sulfation were chemically modified to obtain thiolated analogues (tGAGs) for subsequent surface grafting on vinyl-terminated self-assembled monolayers.
25263900	2	45	theme	subsequent	390:399	arg1	reduction					401:409	subsequent reduction	390:409	subsequent reduction of the disulfide with dithiothreitol	390:446	Thiolation was achieved by the use of the disulfide containing crosslinker 3,3'-dithiobis(propanoic hydrazide) and subsequent reduction of the disulfide with dithiothreitol.
25263900	1	46	theme	vinyl-terminated	231:246	arg1	monolayers					263:272	vinyl-terminated self-assembled monolayers	231:272	vinyl-terminated self-assembled monolayers	231:272	Glycosaminoglycans (GAGs) with varying degree of sulfation were chemically modified to obtain thiolated analogues (tGAGs) for subsequent surface grafting on vinyl-terminated self-assembled monolayers.
25263900	5	47	used	used	718:721	arg2	reagent					706:712	Ellman's reagent	697:712	Ellman's reagent	697:712	Ellman's reagent was used to quantify the thiol concentration of tGAGs.
25263900	4	48	dep	H-NMR	557:561	arg1	1					555:555	1	555:555	1	555:555	The tGAGs were characterized by (1)H-NMR, Raman and flow-field-flow-fractionation (A4F) to determine the chemical composition, structure and molecular weight of the products.
25263900	4	49	theme	chemical	627:634	arg1	composition					636:646	the chemical composition	623:646	the chemical composition	623:646	The tGAGs were characterized by (1)H-NMR, Raman and flow-field-flow-fractionation (A4F) to determine the chemical composition, structure and molecular weight of the products.
25263900	1	50	theme	self-assembled	248:261	arg1	monolayers					263:272	vinyl-terminated self-assembled monolayers	231:272	vinyl-terminated self-assembled monolayers	231:272	Glycosaminoglycans (GAGs) with varying degree of sulfation were chemically modified to obtain thiolated analogues (tGAGs) for subsequent surface grafting on vinyl-terminated self-assembled monolayers.
25285759	3	0	theme	carbohydrate	650:661	arg1	contents					663:670	the carbohydrate contents	646:670	the carbohydrate contents	646:670	After 10-day culture, addition of 0.5-5.0 g L(-1) glucose and galactose significantly reduced the cellular protein contents by 27.7-63.7% and 22.6-60.5%, respectively, and significantly increased the carbohydrate contents by 103.2-266.5% and 91.9-240.0%, respectively.
25285759	1	1	from	effects	155:161	arg1	growth					273:278	growth	273:278	growth	273:278	The aim of this study was to investigate the effects of various organic carbon sources (glucose, galactose, fructose, sucrose, maltose, lactose and starch) on the growth and biochemical composition of Chlorella pyrenoidosa.
25285759	1	1	from	effects	155:161	arg1	composition					296:306	biochemical composition	284:306	biochemical composition	284:306	The aim of this study was to investigate the effects of various organic carbon sources (glucose, galactose, fructose, sucrose, maltose, lactose and starch) on the growth and biochemical composition of Chlorella pyrenoidosa.
25285759	0	2	theme	biochemical	60:70	arg1	composition					72:82	biochemical composition	60:82	biochemical composition	60:82	Effects of various organic carbon sources on the growth and biochemical composition of Chlorella pyrenoidosa.
25285759	1	3	theme	biochemical	284:294	arg1	composition					296:306	biochemical composition	284:306	biochemical composition	284:306	The aim of this study was to investigate the effects of various organic carbon sources (glucose, galactose, fructose, sucrose, maltose, lactose and starch) on the growth and biochemical composition of Chlorella pyrenoidosa.
25285759	0	4	theme	Chlorella	87:95	arg1	pyrenoidosa					97:107	Chlorella pyrenoidosa	87:107	Chlorella pyrenoidosa	87:107	Effects of various organic carbon sources on the growth and biochemical composition of Chlorella pyrenoidosa.
25285759	0	5	from	Effects	0:6	arg1	growth					49:54	growth	49:54	growth	49:54	Effects of various organic carbon sources on the growth and biochemical composition of Chlorella pyrenoidosa.
25285759	0	5	from	Effects	0:6	arg1	composition					72:82	biochemical composition	60:82	biochemical composition	60:82	Effects of various organic carbon sources on the growth and biochemical composition of Chlorella pyrenoidosa.
25285759	5	6	theme	nitrogen	881:888	arg1	condition					890:898	the normal nitrogen condition	870:898	the normal nitrogen condition	870:898	Similar to the normal nitrogen condition, the cellular biochemical composition was not significantly affected by addition of 3.0 g L(-1) disaccharides and starch under the low nitrogen condition.
25285759	3	7	theme	glucose	500:506	arg1	addition					472:479	addition	472:479	addition of 0.5-5.0 g L(-1) glucose and galactose	472:520	After 10-day culture, addition of 0.5-5.0 g L(-1) glucose and galactose significantly reduced the cellular protein contents by 27.7-63.7% and 22.6-60.5%, respectively, and significantly increased the carbohydrate contents by 103.2-266.5% and 91.9-240.0%, respectively.
25285759	5	8	theme	disaccharides	996:1008	arg1	addition					972:979	addition	972:979	addition of 3.0 g L(-1) disaccharides and starch	972:1019	Similar to the normal nitrogen condition, the cellular biochemical composition was not significantly affected by addition of 3.0 g L(-1) disaccharides and starch under the low nitrogen condition.
25285759	6	9	theme	biotechnological	1101:1116	arg1	application					1118:1128	the biotechnological application	1097:1128	the biotechnological application of mixotrophic cultivation of C. pyrenoidosa	1097:1173	Finally, the significance of this work in the biotechnological application of mixotrophic cultivation of C. pyrenoidosa was further discussed.
25285759	1	10	theme	Chlorella	311:319	arg1	pyrenoidosa					321:331	Chlorella pyrenoidosa	311:331	Chlorella pyrenoidosa	311:331	The aim of this study was to investigate the effects of various organic carbon sources (glucose, galactose, fructose, sucrose, maltose, lactose and starch) on the growth and biochemical composition of Chlorella pyrenoidosa.
25285759	2	11	theme	stimulative	379:389	arg1	effects					391:397	stronger stimulative effects	370:397	stronger stimulative effects	370:397	Monosaccharides were found to exert stronger stimulative effects on the algal growth than disaccharides and starch.
25285759	3	12	theme	g	492:492	arg1	-1					496:497	-1	496:497	-1	496:497	After 10-day culture, addition of 0.5-5.0 g L(-1) glucose and galactose significantly reduced the cellular protein contents by 27.7-63.7% and 22.6-60.5%, respectively, and significantly increased the carbohydrate contents by 103.2-266.5% and 91.9-240.0%, respectively.
25285759	3	12	theme	g	492:492	arg1	L					494:494	0.5-5.0 g L	484:494	0.5-5.0 g L(-1) glucose	484:506	After 10-day culture, addition of 0.5-5.0 g L(-1) glucose and galactose significantly reduced the cellular protein contents by 27.7-63.7% and 22.6-60.5%, respectively, and significantly increased the carbohydrate contents by 103.2-266.5% and 91.9-240.0%, respectively.
25285759	6	13	from	significance	1068:1079	arg1	application					1118:1128	the biotechnological application	1097:1128	the biotechnological application of mixotrophic cultivation of C. pyrenoidosa	1097:1173	Finally, the significance of this work in the biotechnological application of mixotrophic cultivation of C. pyrenoidosa was further discussed.
25285759	0	14	theme	pyrenoidosa	97:107	arg1	growth					49:54	growth	49:54	growth	49:54	Effects of various organic carbon sources on the growth and biochemical composition of Chlorella pyrenoidosa.
25285759	0	14	theme	pyrenoidosa	97:107	arg1	composition					72:82	biochemical composition	60:82	biochemical composition	60:82	Effects of various organic carbon sources on the growth and biochemical composition of Chlorella pyrenoidosa.
25285759	1	15	theme	pyrenoidosa	321:331	arg1	growth					273:278	growth	273:278	growth	273:278	The aim of this study was to investigate the effects of various organic carbon sources (glucose, galactose, fructose, sucrose, maltose, lactose and starch) on the growth and biochemical composition of Chlorella pyrenoidosa.
25285759	1	15	theme	pyrenoidosa	321:331	arg1	composition					296:306	biochemical composition	284:306	biochemical composition	284:306	The aim of this study was to investigate the effects of various organic carbon sources (glucose, galactose, fructose, sucrose, maltose, lactose and starch) on the growth and biochemical composition of Chlorella pyrenoidosa.
25285759	4	16	theme	disaccharides	756:768	arg1	addition					728:735	addition	728:735	addition of 0.5-5.0 g L(-1) disaccharides and starch	728:779	However, addition of 0.5-5.0 g L(-1) disaccharides and starch did not significantly affect the contents of lipid, protein and carbohydrate.
25285759	3	17	theme	L	494:494	arg1	glucose					500:506	0.5-5.0 g L(-1) glucose	484:506	0.5-5.0 g L(-1) glucose	484:506	After 10-day culture, addition of 0.5-5.0 g L(-1) glucose and galactose significantly reduced the cellular protein contents by 27.7-63.7% and 22.6-60.5%, respectively, and significantly increased the carbohydrate contents by 103.2-266.5% and 91.9-240.0%, respectively.
25285759	6	18	theme	pyrenoidosa	1163:1173	arg1	cultivation					1145:1155	mixotrophic cultivation	1133:1155	mixotrophic cultivation of C. pyrenoidosa	1133:1173	Finally, the significance of this work in the biotechnological application of mixotrophic cultivation of C. pyrenoidosa was further discussed.
25285759	6	19	theme	cultivation	1145:1155	arg1	application					1118:1128	the biotechnological application	1097:1128	the biotechnological application of mixotrophic cultivation of C. pyrenoidosa	1097:1173	Finally, the significance of this work in the biotechnological application of mixotrophic cultivation of C. pyrenoidosa was further discussed.
25285759	2	20	theme	algal	406:410	arg1	growth					412:417	the algal growth	402:417	the algal growth than disaccharides and starch	402:447	Monosaccharides were found to exert stronger stimulative effects on the algal growth than disaccharides and starch.
25285759	4	21	theme	starch	774:779	arg1	addition					728:735	addition	728:735	addition of 0.5-5.0 g L(-1) disaccharides and starch	728:779	However, addition of 0.5-5.0 g L(-1) disaccharides and starch did not significantly affect the contents of lipid, protein and carbohydrate.
25285759	3	22	theme	galactose	512:520	arg1	addition					472:479	addition	472:479	addition of 0.5-5.0 g L(-1) glucose and galactose	472:520	After 10-day culture, addition of 0.5-5.0 g L(-1) glucose and galactose significantly reduced the cellular protein contents by 27.7-63.7% and 22.6-60.5%, respectively, and significantly increased the carbohydrate contents by 103.2-266.5% and 91.9-240.0%, respectively.
25285759	5	23	theme	nitrogen	1035:1042	arg1	condition					1044:1052	the low nitrogen condition	1027:1052	the low nitrogen condition	1027:1052	Similar to the normal nitrogen condition, the cellular biochemical composition was not significantly affected by addition of 3.0 g L(-1) disaccharides and starch under the low nitrogen condition.
25285759	1	24	theme	various	166:172	arg1	glucose					198:204	glucose	198:204	glucose	198:204	The aim of this study was to investigate the effects of various organic carbon sources (glucose, galactose, fructose, sucrose, maltose, lactose and starch) on the growth and biochemical composition of Chlorella pyrenoidosa.
25285759	1	24	theme	various	166:172	arg1	galactose					207:215	galactose	207:215	galactose	207:215	The aim of this study was to investigate the effects of various organic carbon sources (glucose, galactose, fructose, sucrose, maltose, lactose and starch) on the growth and biochemical composition of Chlorella pyrenoidosa.
25285759	1	24	theme	various	166:172	arg1	maltose					237:243	maltose	237:243	maltose	237:243	The aim of this study was to investigate the effects of various organic carbon sources (glucose, galactose, fructose, sucrose, maltose, lactose and starch) on the growth and biochemical composition of Chlorella pyrenoidosa.
25285759	1	24	theme	various	166:172	arg1	lactose					246:252	lactose	246:252	lactose	246:252	The aim of this study was to investigate the effects of various organic carbon sources (glucose, galactose, fructose, sucrose, maltose, lactose and starch) on the growth and biochemical composition of Chlorella pyrenoidosa.
25285759	1	24	theme	various	166:172	arg1	sources					189:195	various organic carbon sources	166:195	various organic carbon sources (glucose, galactose, fructose, sucrose, maltose, lactose and starch)	166:264	The aim of this study was to investigate the effects of various organic carbon sources (glucose, galactose, fructose, sucrose, maltose, lactose and starch) on the growth and biochemical composition of Chlorella pyrenoidosa.
25285759	1	24	theme	various	166:172	arg1	fructose					218:225	fructose	218:225	fructose	218:225	The aim of this study was to investigate the effects of various organic carbon sources (glucose, galactose, fructose, sucrose, maltose, lactose and starch) on the growth and biochemical composition of Chlorella pyrenoidosa.
25285759	1	24	theme	various	166:172	arg1	sucrose					228:234	sucrose	228:234	sucrose	228:234	The aim of this study was to investigate the effects of various organic carbon sources (glucose, galactose, fructose, sucrose, maltose, lactose and starch) on the growth and biochemical composition of Chlorella pyrenoidosa.
25285759	1	24	theme	various	166:172	arg1	starch					258:263	starch	258:263	starch	258:263	The aim of this study was to investigate the effects of various organic carbon sources (glucose, galactose, fructose, sucrose, maltose, lactose and starch) on the growth and biochemical composition of Chlorella pyrenoidosa.
25285759	0	25	theme	organic	19:25	arg1	sources					34:40	various organic carbon sources	11:40	various organic carbon sources	11:40	Effects of various organic carbon sources on the growth and biochemical composition of Chlorella pyrenoidosa.
25285759	4	26	theme	g	748:748	arg1	-1					752:753	-1	752:753	-1	752:753	However, addition of 0.5-5.0 g L(-1) disaccharides and starch did not significantly affect the contents of lipid, protein and carbohydrate.
25285759	4	26	theme	g	748:748	arg1	L					750:750	0.5-5.0 g L	740:750	0.5-5.0 g L(-1) disaccharides	740:768	However, addition of 0.5-5.0 g L(-1) disaccharides and starch did not significantly affect the contents of lipid, protein and carbohydrate.
25285759	5	27	theme	normal	874:879	arg1	condition					890:898	the normal nitrogen condition	870:898	the normal nitrogen condition	870:898	Similar to the normal nitrogen condition, the cellular biochemical composition was not significantly affected by addition of 3.0 g L(-1) disaccharides and starch under the low nitrogen condition.
25285759	1	28	theme	organic	174:180	arg1	glucose					198:204	glucose	198:204	glucose	198:204	The aim of this study was to investigate the effects of various organic carbon sources (glucose, galactose, fructose, sucrose, maltose, lactose and starch) on the growth and biochemical composition of Chlorella pyrenoidosa.
25285759	1	28	theme	organic	174:180	arg1	galactose					207:215	galactose	207:215	galactose	207:215	The aim of this study was to investigate the effects of various organic carbon sources (glucose, galactose, fructose, sucrose, maltose, lactose and starch) on the growth and biochemical composition of Chlorella pyrenoidosa.
25285759	1	28	theme	organic	174:180	arg1	maltose					237:243	maltose	237:243	maltose	237:243	The aim of this study was to investigate the effects of various organic carbon sources (glucose, galactose, fructose, sucrose, maltose, lactose and starch) on the growth and biochemical composition of Chlorella pyrenoidosa.
25285759	1	28	theme	organic	174:180	arg1	lactose					246:252	lactose	246:252	lactose	246:252	The aim of this study was to investigate the effects of various organic carbon sources (glucose, galactose, fructose, sucrose, maltose, lactose and starch) on the growth and biochemical composition of Chlorella pyrenoidosa.
25285759	1	28	theme	organic	174:180	arg1	sources					189:195	various organic carbon sources	166:195	various organic carbon sources (glucose, galactose, fructose, sucrose, maltose, lactose and starch)	166:264	The aim of this study was to investigate the effects of various organic carbon sources (glucose, galactose, fructose, sucrose, maltose, lactose and starch) on the growth and biochemical composition of Chlorella pyrenoidosa.
25285759	1	28	theme	organic	174:180	arg1	fructose					218:225	fructose	218:225	fructose	218:225	The aim of this study was to investigate the effects of various organic carbon sources (glucose, galactose, fructose, sucrose, maltose, lactose and starch) on the growth and biochemical composition of Chlorella pyrenoidosa.
25285759	1	28	theme	organic	174:180	arg1	sucrose					228:234	sucrose	228:234	sucrose	228:234	The aim of this study was to investigate the effects of various organic carbon sources (glucose, galactose, fructose, sucrose, maltose, lactose and starch) on the growth and biochemical composition of Chlorella pyrenoidosa.
25285759	1	28	theme	organic	174:180	arg1	starch					258:263	starch	258:263	starch	258:263	The aim of this study was to investigate the effects of various organic carbon sources (glucose, galactose, fructose, sucrose, maltose, lactose and starch) on the growth and biochemical composition of Chlorella pyrenoidosa.
25285759	0	29	theme	various	11:17	arg1	sources					34:40	various organic carbon sources	11:40	various organic carbon sources	11:40	Effects of various organic carbon sources on the growth and biochemical composition of Chlorella pyrenoidosa.
25285759	1	30	dep	growth	273:278	arg1	the					269:271	the	269:271	the	269:271	The aim of this study was to investigate the effects of various organic carbon sources (glucose, galactose, fructose, sucrose, maltose, lactose and starch) on the growth and biochemical composition of Chlorella pyrenoidosa.
25285759	4	31	theme	protein	833:839	arg1	contents					814:821	the contents	810:821	the contents of lipid, protein and carbohydrate	810:856	However, addition of 0.5-5.0 g L(-1) disaccharides and starch did not significantly affect the contents of lipid, protein and carbohydrate.
25285759	5	32	theme	L	990:990	arg1	disaccharides					996:1008	3.0 g L(-1) disaccharides	984:1008	3.0 g L(-1) disaccharides	984:1008	Similar to the normal nitrogen condition, the cellular biochemical composition was not significantly affected by addition of 3.0 g L(-1) disaccharides and starch under the low nitrogen condition.
25285759	0	33	dep	growth	49:54	arg1	the					45:47	the	45:47	the	45:47	Effects of various organic carbon sources on the growth and biochemical composition of Chlorella pyrenoidosa.
25285759	1	34	theme	carbon	182:187	arg1	glucose					198:204	glucose	198:204	glucose	198:204	The aim of this study was to investigate the effects of various organic carbon sources (glucose, galactose, fructose, sucrose, maltose, lactose and starch) on the growth and biochemical composition of Chlorella pyrenoidosa.
25285759	1	34	theme	carbon	182:187	arg1	galactose					207:215	galactose	207:215	galactose	207:215	The aim of this study was to investigate the effects of various organic carbon sources (glucose, galactose, fructose, sucrose, maltose, lactose and starch) on the growth and biochemical composition of Chlorella pyrenoidosa.
25285759	1	34	theme	carbon	182:187	arg1	maltose					237:243	maltose	237:243	maltose	237:243	The aim of this study was to investigate the effects of various organic carbon sources (glucose, galactose, fructose, sucrose, maltose, lactose and starch) on the growth and biochemical composition of Chlorella pyrenoidosa.
25285759	1	34	theme	carbon	182:187	arg1	lactose					246:252	lactose	246:252	lactose	246:252	The aim of this study was to investigate the effects of various organic carbon sources (glucose, galactose, fructose, sucrose, maltose, lactose and starch) on the growth and biochemical composition of Chlorella pyrenoidosa.
25285759	1	34	theme	carbon	182:187	arg1	sources					189:195	various organic carbon sources	166:195	various organic carbon sources (glucose, galactose, fructose, sucrose, maltose, lactose and starch)	166:264	The aim of this study was to investigate the effects of various organic carbon sources (glucose, galactose, fructose, sucrose, maltose, lactose and starch) on the growth and biochemical composition of Chlorella pyrenoidosa.
25285759	1	34	theme	carbon	182:187	arg1	fructose					218:225	fructose	218:225	fructose	218:225	The aim of this study was to investigate the effects of various organic carbon sources (glucose, galactose, fructose, sucrose, maltose, lactose and starch) on the growth and biochemical composition of Chlorella pyrenoidosa.
25285759	1	34	theme	carbon	182:187	arg1	sucrose					228:234	sucrose	228:234	sucrose	228:234	The aim of this study was to investigate the effects of various organic carbon sources (glucose, galactose, fructose, sucrose, maltose, lactose and starch) on the growth and biochemical composition of Chlorella pyrenoidosa.
25285759	1	34	theme	carbon	182:187	arg1	starch					258:263	starch	258:263	starch	258:263	The aim of this study was to investigate the effects of various organic carbon sources (glucose, galactose, fructose, sucrose, maltose, lactose and starch) on the growth and biochemical composition of Chlorella pyrenoidosa.
25285759	0	35	theme	sources	34:40	arg1	Effects					0:6	Effects	0:6	Effects of various organic carbon sources on the growth and biochemical composition of Chlorella pyrenoidosa	0:107	Effects of various organic carbon sources on the growth and biochemical composition of Chlorella pyrenoidosa.
25285759	1	36	theme	sources	189:195	arg1	effects					155:161	the effects	151:161	the effects of various organic carbon sources (glucose, galactose, fructose, sucrose, maltose, lactose and starch) on the growth and biochemical composition of Chlorella pyrenoidosa	151:331	The aim of this study was to investigate the effects of various organic carbon sources (glucose, galactose, fructose, sucrose, maltose, lactose and starch) on the growth and biochemical composition of Chlorella pyrenoidosa.
25285759	2	37	theme	stronger	370:377	arg1	effects					391:397	stronger stimulative effects	370:397	stronger stimulative effects	370:397	Monosaccharides were found to exert stronger stimulative effects on the algal growth than disaccharides and starch.
25285759	0	38	theme	carbon	27:32	arg1	sources					34:40	various organic carbon sources	11:40	various organic carbon sources	11:40	Effects of various organic carbon sources on the growth and biochemical composition of Chlorella pyrenoidosa.
25285759	4	39	theme	carbohydrate	845:856	arg1	contents					814:821	the contents	810:821	the contents of lipid, protein and carbohydrate	810:856	However, addition of 0.5-5.0 g L(-1) disaccharides and starch did not significantly affect the contents of lipid, protein and carbohydrate.
25285759	3	40	theme	cellular	548:555	arg1	contents					565:572	the cellular protein contents	544:572	the cellular protein contents	544:572	After 10-day culture, addition of 0.5-5.0 g L(-1) glucose and galactose significantly reduced the cellular protein contents by 27.7-63.7% and 22.6-60.5%, respectively, and significantly increased the carbohydrate contents by 103.2-266.5% and 91.9-240.0%, respectively.
25285759	5	41	theme	low	1031:1033	arg1	condition					1044:1052	the low nitrogen condition	1027:1052	the low nitrogen condition	1027:1052	Similar to the normal nitrogen condition, the cellular biochemical composition was not significantly affected by addition of 3.0 g L(-1) disaccharides and starch under the low nitrogen condition.
25285759	6	42	theme	mixotrophic	1133:1143	arg1	cultivation					1145:1155	mixotrophic cultivation	1133:1155	mixotrophic cultivation of C. pyrenoidosa	1133:1173	Finally, the significance of this work in the biotechnological application of mixotrophic cultivation of C. pyrenoidosa was further discussed.
25285759	5	43	theme	starch	1014:1019	arg1	addition					972:979	addition	972:979	addition of 3.0 g L(-1) disaccharides and starch	972:1019	Similar to the normal nitrogen condition, the cellular biochemical composition was not significantly affected by addition of 3.0 g L(-1) disaccharides and starch under the low nitrogen condition.
25285759	3	44	theme	10-day	456:461	arg1	culture					463:469	10-day culture	456:469	10-day culture	456:469	After 10-day culture, addition of 0.5-5.0 g L(-1) glucose and galactose significantly reduced the cellular protein contents by 27.7-63.7% and 22.6-60.5%, respectively, and significantly increased the carbohydrate contents by 103.2-266.5% and 91.9-240.0%, respectively.
25285759	5	45	theme	cellular	905:912	arg1	composition					926:936	the cellular biochemical composition	901:936	the cellular biochemical composition	901:936	Similar to the normal nitrogen condition, the cellular biochemical composition was not significantly affected by addition of 3.0 g L(-1) disaccharides and starch under the low nitrogen condition.
25285759	1	46	dep	sources	189:195	arg1	glucose					198:204	glucose	198:204	glucose	198:204	The aim of this study was to investigate the effects of various organic carbon sources (glucose, galactose, fructose, sucrose, maltose, lactose and starch) on the growth and biochemical composition of Chlorella pyrenoidosa.
25285759	1	46	dep	sources	189:195	arg1	galactose					207:215	galactose	207:215	galactose	207:215	The aim of this study was to investigate the effects of various organic carbon sources (glucose, galactose, fructose, sucrose, maltose, lactose and starch) on the growth and biochemical composition of Chlorella pyrenoidosa.
25285759	1	46	dep	sources	189:195	arg1	maltose					237:243	maltose	237:243	maltose	237:243	The aim of this study was to investigate the effects of various organic carbon sources (glucose, galactose, fructose, sucrose, maltose, lactose and starch) on the growth and biochemical composition of Chlorella pyrenoidosa.
25285759	1	46	dep	sources	189:195	arg1	lactose					246:252	lactose	246:252	lactose	246:252	The aim of this study was to investigate the effects of various organic carbon sources (glucose, galactose, fructose, sucrose, maltose, lactose and starch) on the growth and biochemical composition of Chlorella pyrenoidosa.
25285759	1	46	dep	sources	189:195	arg1	sources					189:195	various organic carbon sources	166:195	various organic carbon sources (glucose, galactose, fructose, sucrose, maltose, lactose and starch)	166:264	The aim of this study was to investigate the effects of various organic carbon sources (glucose, galactose, fructose, sucrose, maltose, lactose and starch) on the growth and biochemical composition of Chlorella pyrenoidosa.
25285759	1	46	dep	sources	189:195	arg1	fructose					218:225	fructose	218:225	fructose	218:225	The aim of this study was to investigate the effects of various organic carbon sources (glucose, galactose, fructose, sucrose, maltose, lactose and starch) on the growth and biochemical composition of Chlorella pyrenoidosa.
25285759	1	46	dep	sources	189:195	arg1	sucrose					228:234	sucrose	228:234	sucrose	228:234	The aim of this study was to investigate the effects of various organic carbon sources (glucose, galactose, fructose, sucrose, maltose, lactose and starch) on the growth and biochemical composition of Chlorella pyrenoidosa.
25285759	1	46	dep	sources	189:195	arg1	starch					258:263	starch	258:263	starch	258:263	The aim of this study was to investigate the effects of various organic carbon sources (glucose, galactose, fructose, sucrose, maltose, lactose and starch) on the growth and biochemical composition of Chlorella pyrenoidosa.
25285759	4	47	theme	L	750:750	arg1	disaccharides					756:768	0.5-5.0 g L(-1) disaccharides	740:768	0.5-5.0 g L(-1) disaccharides	740:768	However, addition of 0.5-5.0 g L(-1) disaccharides and starch did not significantly affect the contents of lipid, protein and carbohydrate.
25285759	4	48	theme	lipid	826:830	arg1	contents					814:821	the contents	810:821	the contents of lipid, protein and carbohydrate	810:856	However, addition of 0.5-5.0 g L(-1) disaccharides and starch did not significantly affect the contents of lipid, protein and carbohydrate.
25285759	5	49	theme	biochemical	914:924	arg1	composition					926:936	the cellular biochemical composition	901:936	the cellular biochemical composition	901:936	Similar to the normal nitrogen condition, the cellular biochemical composition was not significantly affected by addition of 3.0 g L(-1) disaccharides and starch under the low nitrogen condition.
25285759	5	50	theme	g	988:988	arg1	-1					992:993	-1	992:993	-1	992:993	Similar to the normal nitrogen condition, the cellular biochemical composition was not significantly affected by addition of 3.0 g L(-1) disaccharides and starch under the low nitrogen condition.
25285759	5	50	theme	g	988:988	arg1	L					990:990	3.0 g L	984:990	3.0 g L(-1) disaccharides	984:1008	Similar to the normal nitrogen condition, the cellular biochemical composition was not significantly affected by addition of 3.0 g L(-1) disaccharides and starch under the low nitrogen condition.
25285759	3	51	theme	protein	557:563	arg1	contents					565:572	the cellular protein contents	544:572	the cellular protein contents	544:572	After 10-day culture, addition of 0.5-5.0 g L(-1) glucose and galactose significantly reduced the cellular protein contents by 27.7-63.7% and 22.6-60.5%, respectively, and significantly increased the carbohydrate contents by 103.2-266.5% and 91.9-240.0%, respectively.
25285759	1	52	theme	study	126:130	arg1	aim					114:116	The aim	110:116	The aim of this study	110:130	The aim of this study was to investigate the effects of various organic carbon sources (glucose, galactose, fructose, sucrose, maltose, lactose and starch) on the growth and biochemical composition of Chlorella pyrenoidosa.
25285759	6	53	theme	work	1089:1092	arg1	significance					1068:1079	the significance	1064:1079	the significance of this work in the biotechnological application of mixotrophic cultivation of C. pyrenoidosa	1064:1173	Finally, the significance of this work in the biotechnological application of mixotrophic cultivation of C. pyrenoidosa was further discussed.
29054524	1	0	theme	Plantago	263:270	arg1	solutions					304:312	Plantago lanceolata seed mucilage (PLSM) solutions	263:312	Plantago lanceolata seed mucilage (PLSM) solutions	263:312	In this study, the effect of concentration (0.5, 1, 1.5 and 2%) and heating-cooling rate (1, 5 and 10 °C min-1) on the rheological properties of Plantago lanceolata seed mucilage (PLSM) solutions were investigated.
29054524	1	1	theme	rate	202:205	arg1	effect					137:142	the effect	133:142	the effect of concentration (0.5, 1, 1.5 and 2%) and heating-cooling rate (1, 5 and 10 °C min-1) on the rheological properties of Plantago lanceolata seed mucilage (PLSM) solutions	133:312	In this study, the effect of concentration (0.5, 1, 1.5 and 2%) and heating-cooling rate (1, 5 and 10 °C min-1) on the rheological properties of Plantago lanceolata seed mucilage (PLSM) solutions were investigated.
29054524	2	2	theme	given	418:422	arg1	conditions					424:433	the given conditions	414:433	the given conditions	414:433	It was observed that the gum dispersions exhibited viscoelastic properties under the given conditions.
29054524	0	3	from	effects	4:10	arg1	properties					69:78	rheological properties	57:78	rheological properties of Plantago lanceolata	57:101	The effects of concentration and heating-cooling rate on rheological properties of Plantago lanceolata seed mucilage.
29054524	1	4	theme	concentration	147:159	arg1	effect					137:142	the effect	133:142	the effect of concentration (0.5, 1, 1.5 and 2%) and heating-cooling rate (1, 5 and 10 °C min-1) on the rheological properties of Plantago lanceolata seed mucilage (PLSM) solutions	133:312	In this study, the effect of concentration (0.5, 1, 1.5 and 2%) and heating-cooling rate (1, 5 and 10 °C min-1) on the rheological properties of Plantago lanceolata seed mucilage (PLSM) solutions were investigated.
29054524	3	5	theme	δ	553:553	arg1	results					555:561	the frequency sweep, complex viscosity (η*) and tan δ results	501:561	the frequency sweep, complex viscosity (η*) and tan δ results	501:561	Mechanical spectra of PLSM were classified as weak gels based on the frequency sweep, complex viscosity (η*) and tan δ results.
29054524	1	6	theme	seed	283:286	arg1	solutions					304:312	Plantago lanceolata seed mucilage (PLSM) solutions	263:312	Plantago lanceolata seed mucilage (PLSM) solutions	263:312	In this study, the effect of concentration (0.5, 1, 1.5 and 2%) and heating-cooling rate (1, 5 and 10 °C min-1) on the rheological properties of Plantago lanceolata seed mucilage (PLSM) solutions were investigated.
29054524	3	7	theme	weak	482:485	arg1	gels					487:490	weak gels	482:490	weak gels based on the frequency sweep, complex viscosity (η*) and tan δ results	482:561	Mechanical spectra of PLSM were classified as weak gels based on the frequency sweep, complex viscosity (η*) and tan δ results.
29054524	3	7	theme	weak	482:485	arg1	spectra					447:453	Mechanical spectra	436:453	Mechanical spectra of PLSM	436:461	Mechanical spectra of PLSM were classified as weak gels based on the frequency sweep, complex viscosity (η*) and tan δ results.
29054524	0	8	theme	lanceolata	92:101	arg1	properties					69:78	rheological properties	57:78	rheological properties of Plantago lanceolata	57:101	The effects of concentration and heating-cooling rate on rheological properties of Plantago lanceolata seed mucilage.
29054524	1	9	theme	mucilage	288:295	arg1	solutions					304:312	Plantago lanceolata seed mucilage (PLSM) solutions	263:312	Plantago lanceolata seed mucilage (PLSM) solutions	263:312	In this study, the effect of concentration (0.5, 1, 1.5 and 2%) and heating-cooling rate (1, 5 and 10 °C min-1) on the rheological properties of Plantago lanceolata seed mucilage (PLSM) solutions were investigated.
29054524	2	10	theme	gum	358:360	arg1	dispersions					362:372	the gum dispersions	354:372	the gum dispersions	354:372	It was observed that the gum dispersions exhibited viscoelastic properties under the given conditions.
29054524	1	11	from	effect	137:142	arg1	properties					249:258	the rheological properties	233:258	the rheological properties of Plantago lanceolata seed mucilage (PLSM) solutions	233:312	In this study, the effect of concentration (0.5, 1, 1.5 and 2%) and heating-cooling rate (1, 5 and 10 °C min-1) on the rheological properties of Plantago lanceolata seed mucilage (PLSM) solutions were investigated.
29054524	0	12	theme	rate	49:52	arg1	effects					4:10	The effects	0:10	The effects of concentration and heating-cooling rate on rheological properties of Plantago lanceolata	0:101	The effects of concentration and heating-cooling rate on rheological properties of Plantago lanceolata seed mucilage.
29054524	1	13	theme	PLSM	298:301	arg1	solutions					304:312	Plantago lanceolata seed mucilage (PLSM) solutions	263:312	Plantago lanceolata seed mucilage (PLSM) solutions	263:312	In this study, the effect of concentration (0.5, 1, 1.5 and 2%) and heating-cooling rate (1, 5 and 10 °C min-1) on the rheological properties of Plantago lanceolata seed mucilage (PLSM) solutions were investigated.
29054524	5	14	theme	more	706:709	arg1	information					722:732	more structural information	706:732	more structural information	706:732	Chemical and monosaccharide compositions were also determined to provide more structural information.
29054524	6	15	theme	sugar	781:785	arg1	content					787:793	high total sugar content	770:793	high total sugar content (87.35%)	770:802	The results revealed that PLSM had high total sugar content (87.35%), and it is likely an arabinoxylomannan-type polysaccharide.
29054524	6	15	theme	sugar	781:785	arg1	%					801:801	87.35%	796:801	87.35%	796:801	The results revealed that PLSM had high total sugar content (87.35%), and it is likely an arabinoxylomannan-type polysaccharide.
29054524	1	16	theme	lanceolata	272:281	arg1	solutions					304:312	Plantago lanceolata seed mucilage (PLSM) solutions	263:312	Plantago lanceolata seed mucilage (PLSM) solutions	263:312	In this study, the effect of concentration (0.5, 1, 1.5 and 2%) and heating-cooling rate (1, 5 and 10 °C min-1) on the rheological properties of Plantago lanceolata seed mucilage (PLSM) solutions were investigated.
29054524	3	17	theme	frequency	505:513	arg1	sweep					515:519	frequency sweep	505:519	frequency sweep	505:519	Mechanical spectra of PLSM were classified as weak gels based on the frequency sweep, complex viscosity (η*) and tan δ results.
29054524	0	18	theme	concentration	15:27	arg1	effects					4:10	The effects	0:10	The effects of concentration and heating-cooling rate on rheological properties of Plantago lanceolata	0:101	The effects of concentration and heating-cooling rate on rheological properties of Plantago lanceolata seed mucilage.
29054524	1	19	theme	solutions	304:312	arg1	properties					249:258	the rheological properties	233:258	the rheological properties of Plantago lanceolata seed mucilage (PLSM) solutions	233:312	In this study, the effect of concentration (0.5, 1, 1.5 and 2%) and heating-cooling rate (1, 5 and 10 °C min-1) on the rheological properties of Plantago lanceolata seed mucilage (PLSM) solutions were investigated.
29054524	0	20	theme	heating-cooling	33:47	arg1	rate					49:52	heating-cooling rate	33:52	heating-cooling rate	33:52	The effects of concentration and heating-cooling rate on rheological properties of Plantago lanceolata seed mucilage.
29054524	4	21	theme	rheological	609:619	arg1	parameters					621:630	the rheological parameters	605:630	the rheological parameters	605:630	All variables had significant impacts on the rheological parameters.
29054524	3	22	theme	complex	522:528	arg1	viscosity					530:538	complex viscosity	522:538	complex viscosity (η*)	522:543	Mechanical spectra of PLSM were classified as weak gels based on the frequency sweep, complex viscosity (η*) and tan δ results.
29054524	3	22	theme	complex	522:528	arg1	*					542:542	η*	541:542	η*	541:542	Mechanical spectra of PLSM were classified as weak gels based on the frequency sweep, complex viscosity (η*) and tan δ results.
29054524	5	23	theme	structural	711:720	arg1	information					722:732	more structural information	706:732	more structural information	706:732	Chemical and monosaccharide compositions were also determined to provide more structural information.
29054524	5	24	theme	monosaccharide	646:659	arg1	compositions					661:672	monosaccharide compositions	646:672	monosaccharide compositions	646:672	Chemical and monosaccharide compositions were also determined to provide more structural information.
29054524	3	25	theme	viscosity	530:538	arg1	results					555:561	the frequency sweep, complex viscosity (η*) and tan δ results	501:561	the frequency sweep, complex viscosity (η*) and tan δ results	501:561	Mechanical spectra of PLSM were classified as weak gels based on the frequency sweep, complex viscosity (η*) and tan δ results.
29054524	3	26	theme	tan	549:551	arg1	δ					553:553	tan δ	549:553	tan δ	549:553	Mechanical spectra of PLSM were classified as weak gels based on the frequency sweep, complex viscosity (η*) and tan δ results.
29054524	3	27	theme	sweep	515:519	arg1	results					555:561	the frequency sweep, complex viscosity (η*) and tan δ results	501:561	the frequency sweep, complex viscosity (η*) and tan δ results	501:561	Mechanical spectra of PLSM were classified as weak gels based on the frequency sweep, complex viscosity (η*) and tan δ results.
29054524	2	28	theme	viscoelastic	384:395	arg1	properties					397:406	viscoelastic properties	384:406	viscoelastic properties	384:406	It was observed that the gum dispersions exhibited viscoelastic properties under the given conditions.
29054524	1	29	theme	rheological	237:247	arg1	properties					249:258	the rheological properties	233:258	the rheological properties of Plantago lanceolata seed mucilage (PLSM) solutions	233:312	In this study, the effect of concentration (0.5, 1, 1.5 and 2%) and heating-cooling rate (1, 5 and 10 °C min-1) on the rheological properties of Plantago lanceolata seed mucilage (PLSM) solutions were investigated.
29054524	6	30	theme	total	775:779	arg1	content					787:793	high total sugar content	770:793	high total sugar content (87.35%)	770:802	The results revealed that PLSM had high total sugar content (87.35%), and it is likely an arabinoxylomannan-type polysaccharide.
29054524	6	30	theme	total	775:779	arg1	%					801:801	87.35%	796:801	87.35%	796:801	The results revealed that PLSM had high total sugar content (87.35%), and it is likely an arabinoxylomannan-type polysaccharide.
29054524	6	31	theme	high	770:773	arg1	content					787:793	high total sugar content	770:793	high total sugar content (87.35%)	770:802	The results revealed that PLSM had high total sugar content (87.35%), and it is likely an arabinoxylomannan-type polysaccharide.
29054524	6	31	theme	high	770:773	arg1	%					801:801	87.35%	796:801	87.35%	796:801	The results revealed that PLSM had high total sugar content (87.35%), and it is likely an arabinoxylomannan-type polysaccharide.
29054524	3	32	theme	Mechanical	436:445	arg1	gels					487:490	weak gels	482:490	weak gels based on the frequency sweep, complex viscosity (η*) and tan δ results	482:561	Mechanical spectra of PLSM were classified as weak gels based on the frequency sweep, complex viscosity (η*) and tan δ results.
29054524	3	32	theme	Mechanical	436:445	arg1	spectra					447:453	Mechanical spectra	436:453	Mechanical spectra of PLSM	436:461	Mechanical spectra of PLSM were classified as weak gels based on the frequency sweep, complex viscosity (η*) and tan δ results.
29054524	6	33	theme	arabinoxylomannan-type	825:846	arg1	polysaccharide					848:861	an arabinoxylomannan-type polysaccharide	822:861	an arabinoxylomannan-type polysaccharide	822:861	The results revealed that PLSM had high total sugar content (87.35%), and it is likely an arabinoxylomannan-type polysaccharide.
29054524	6	33	theme	arabinoxylomannan-type	825:846	arg1	it					809:810	it	809:810	it	809:810	The results revealed that PLSM had high total sugar content (87.35%), and it is likely an arabinoxylomannan-type polysaccharide.
29054524	4	34	contain	had	578:580	arg1	variables					568:576	All variables	564:576	All variables	564:576	All variables had significant impacts on the rheological parameters.
29054524	4	34	contain	had	578:580	arg2	impacts					594:600	significant impacts	582:600	significant impacts	582:600	All variables had significant impacts on the rheological parameters.
29054524	6	35	contain	had	766:768	arg2	%					801:801	87.35%	796:801	87.35%	796:801	The results revealed that PLSM had high total sugar content (87.35%), and it is likely an arabinoxylomannan-type polysaccharide.
29054524	6	35	contain	had	766:768	arg1	PLSM					761:764	PLSM	761:764	PLSM	761:764	The results revealed that PLSM had high total sugar content (87.35%), and it is likely an arabinoxylomannan-type polysaccharide.
29054524	6	35	contain	had	766:768	arg2	content					787:793	high total sugar content	770:793	high total sugar content (87.35%)	770:802	The results revealed that PLSM had high total sugar content (87.35%), and it is likely an arabinoxylomannan-type polysaccharide.
29054524	3	36	theme	PLSM	458:461	arg1	gels					487:490	weak gels	482:490	weak gels based on the frequency sweep, complex viscosity (η*) and tan δ results	482:561	Mechanical spectra of PLSM were classified as weak gels based on the frequency sweep, complex viscosity (η*) and tan δ results.
29054524	3	36	theme	PLSM	458:461	arg1	spectra					447:453	Mechanical spectra	436:453	Mechanical spectra of PLSM	436:461	Mechanical spectra of PLSM were classified as weak gels based on the frequency sweep, complex viscosity (η*) and tan δ results.
29054524	0	37	theme	rheological	57:67	arg1	properties					69:78	rheological properties	57:78	rheological properties of Plantago lanceolata	57:101	The effects of concentration and heating-cooling rate on rheological properties of Plantago lanceolata seed mucilage.
29054524	4	38	theme	significant	582:592	arg1	impacts					594:600	significant impacts	582:600	significant impacts	582:600	All variables had significant impacts on the rheological parameters.
29054524	1	39	theme	heating-cooling	186:200	arg1	10 °C min-1					217:227	10 °C min-1	217:227	10 °C min-1	217:227	In this study, the effect of concentration (0.5, 1, 1.5 and 2%) and heating-cooling rate (1, 5 and 10 °C min-1) on the rheological properties of Plantago lanceolata seed mucilage (PLSM) solutions were investigated.
29054524	1	39	theme	heating-cooling	186:200	arg1	1					208:208	1	208:208	1	208:208	In this study, the effect of concentration (0.5, 1, 1.5 and 2%) and heating-cooling rate (1, 5 and 10 °C min-1) on the rheological properties of Plantago lanceolata seed mucilage (PLSM) solutions were investigated.
29054524	1	39	theme	heating-cooling	186:200	arg1	rate					202:205	heating-cooling rate	186:205	heating-cooling rate (1, 5 and 10 °C min-1)	186:228	In this study, the effect of concentration (0.5, 1, 1.5 and 2%) and heating-cooling rate (1, 5 and 10 °C min-1) on the rheological properties of Plantago lanceolata seed mucilage (PLSM) solutions were investigated.
25358771	7	0	theme	determination	1073:1085	arg1	synthesis					1020:1028	the synthesis	1016:1028	the synthesis of nanoparticle-protein cage composite and determination of their magnetic relaxivities by in vitro MR image acquisition and data processing	1016:1169	Here, we describe techniques for the synthesis of nanoparticle-protein cage composite and determination of their magnetic relaxivities by in vitro MR image acquisition and data processing.
25358771	4	1	theme	potential	599:607	arg1	application					609:619	their potential application	593:619	their potential application	593:619	The magnetic nanoparticle-protein cage composites have been reported to have high magnetic relaxivity, which implies their potential application as an MRI contrast agent.
25358771	4	1	theme	potential	599:607	arg1	agent					640:644	an MRI contrast agent	624:644	an MRI contrast agent	624:644	The magnetic nanoparticle-protein cage composites have been reported to have high magnetic relaxivity, which implies their potential application as an MRI contrast agent.
25358771	0	2	theme	resonance	89:97	arg1	imaging					99:105	magnetic resonance imaging	80:105	magnetic resonance imaging	80:105	Determining the relaxivity values of protein cage-templated nanoparticles using magnetic resonance imaging.
25358771	7	3	dep	in	1121:1122	arg1	vitro					1124:1128	vitro	1124:1128	vitro	1124:1128	Here, we describe techniques for the synthesis of nanoparticle-protein cage composite and determination of their magnetic relaxivities by in vitro MR image acquisition and data processing.
25358771	6	4	theme	field	864:868	arg1	relaxometry					870:880	variable field relaxometry	855:880	variable field relaxometry	855:880	The commonly performed techniques are field-cycling NMR relaxometry (also known as variable field relaxometry or nuclear magnetic relaxation dispersion (NMRD) profiling) and in vitro or in vivo MRI relaxometry.
25358771	2	5	theme	complex	349:355	arg1	nanoparticles					369:381	metal complex or metallic nanoparticles	343:381	metal complex or metallic nanoparticles	343:381	This problem can be addressed by developing more sensitive contrast agents by synthesizing new types of metal complex or metallic nanoparticles.
25358771	8	6	theme	nuclei	1292:1297	arg1	rates					1268:1272	relaxation rates	1257:1272	relaxation rates of water hydrogen nuclei	1257:1297	In this method, longitudinal and transverse relaxivities are calculated by measuring relaxation rates of water hydrogen nuclei at different nanoparticle-protein cage composite concentrations.
25358771	6	7	dep	in	946:947	arg1	vitro					949:953	vitro	949:953	vitro	949:953	The commonly performed techniques are field-cycling NMR relaxometry (also known as variable field relaxometry or nuclear magnetic relaxation dispersion (NMRD) profiling) and in vitro or in vivo MRI relaxometry.
25358771	5	8	theme	agent	765:769	arg1	longitudinal					698:709	longitudinal	698:709	longitudinal	698:709	The magnetic relaxivity is determined by measuring longitudinal and transverse magnetic relaxivities of the potential agent.
25358771	6	9	theme	in	946:947	arg1	relaxometry					970:980	in vitro or in vivo MRI relaxometry	946:980	in vitro or in vivo MRI relaxometry	946:980	The commonly performed techniques are field-cycling NMR relaxometry (also known as variable field relaxometry or nuclear magnetic relaxation dispersion (NMRD) profiling) and in vitro or in vivo MRI relaxometry.
25358771	6	10	theme	variable	855:862	arg1	relaxometry					870:880	variable field relaxometry	855:880	variable field relaxometry	855:880	The commonly performed techniques are field-cycling NMR relaxometry (also known as variable field relaxometry or nuclear magnetic relaxation dispersion (NMRD) profiling) and in vitro or in vivo MRI relaxometry.
25358771	2	11	theme	sensitive	288:296	arg1	agents					307:312	more sensitive contrast agents	283:312	more sensitive contrast agents	283:312	This problem can be addressed by developing more sensitive contrast agents by synthesizing new types of metal complex or metallic nanoparticles.
25358771	6	12	dep	in	958:959	arg1	vivo					961:964	vivo	961:964	vivo	961:964	The commonly performed techniques are field-cycling NMR relaxometry (also known as variable field relaxometry or nuclear magnetic relaxation dispersion (NMRD) profiling) and in vitro or in vivo MRI relaxometry.
25358771	8	13	theme	longitudinal	1188:1199	arg1	relaxivities					1216:1227	longitudinal and transverse relaxivities	1188:1227	longitudinal and transverse relaxivities	1188:1227	In this method, longitudinal and transverse relaxivities are calculated by measuring relaxation rates of water hydrogen nuclei at different nanoparticle-protein cage composite concentrations.
25358771	4	14	theme	magnetic	480:487	arg1	composites					515:524	The magnetic nanoparticle-protein cage composites	476:524	The magnetic nanoparticle-protein cage composites	476:524	The magnetic nanoparticle-protein cage composites have been reported to have high magnetic relaxivity, which implies their potential application as an MRI contrast agent.
25358771	7	15	theme	data	1155:1158	arg1	processing					1160:1169	data processing	1155:1169	data processing	1155:1169	Here, we describe techniques for the synthesis of nanoparticle-protein cage composite and determination of their magnetic relaxivities by in vitro MR image acquisition and data processing.
25358771	6	16	dep	relaxometry	828:838	arg1	known					846:850	known	846:850	known as variable field relaxometry or nuclear magnetic relaxation dispersion (NMRD) profiling	846:939	The commonly performed techniques are field-cycling NMR relaxometry (also known as variable field relaxometry or nuclear magnetic relaxation dispersion (NMRD) profiling) and in vitro or in vivo MRI relaxometry.
25358771	5	17	dep	longitudinal	698:709	arg1	relaxivities					735:746	magnetic relaxivities	726:746	magnetic relaxivities	726:746	The magnetic relaxivity is determined by measuring longitudinal and transverse magnetic relaxivities of the potential agent.
25358771	2	18	theme	metal	343:347	arg1	nanoparticles					369:381	metal complex or metallic nanoparticles	343:381	metal complex or metallic nanoparticles	343:381	This problem can be addressed by developing more sensitive contrast agents by synthesizing new types of metal complex or metallic nanoparticles.
25358771	7	19	theme	image	1133:1137	arg1	acquisition					1139:1149	in vitro MR image acquisition	1121:1149	in vitro MR image acquisition	1121:1149	Here, we describe techniques for the synthesis of nanoparticle-protein cage composite and determination of their magnetic relaxivities by in vitro MR image acquisition and data processing.
25358771	8	20	theme	different	1302:1310	arg1	concentrations					1348:1361	different nanoparticle-protein cage composite concentrations	1302:1361	different nanoparticle-protein cage composite concentrations	1302:1361	In this method, longitudinal and transverse relaxivities are calculated by measuring relaxation rates of water hydrogen nuclei at different nanoparticle-protein cage composite concentrations.
25358771	3	21	theme	biological	428:437	arg1	synthesis					439:447	biological synthesis	428:447	biological synthesis of magnetic nanoparticles	428:473	Protein cage has been used as a template in biological synthesis of magnetic nanoparticles.
25358771	8	22	theme	transverse	1205:1214	arg1	relaxivities					1216:1227	longitudinal and transverse relaxivities	1188:1227	longitudinal and transverse relaxivities	1188:1227	In this method, longitudinal and transverse relaxivities are calculated by measuring relaxation rates of water hydrogen nuclei at different nanoparticle-protein cage composite concentrations.
25358771	4	23	theme	cage	510:513	arg1	composites					515:524	The magnetic nanoparticle-protein cage composites	476:524	The magnetic nanoparticle-protein cage composites	476:524	The magnetic nanoparticle-protein cage composites have been reported to have high magnetic relaxivity, which implies their potential application as an MRI contrast agent.
25358771	7	24	theme	in	1121:1122	arg1	acquisition					1139:1149	in vitro MR image acquisition	1121:1149	in vitro MR image acquisition	1121:1149	Here, we describe techniques for the synthesis of nanoparticle-protein cage composite and determination of their magnetic relaxivities by in vitro MR image acquisition and data processing.
25358771	4	25	contain	have	548:551	arg2	relaxivity					567:576	high magnetic relaxivity	553:576	high magnetic relaxivity	553:576	The magnetic nanoparticle-protein cage composites have been reported to have high magnetic relaxivity, which implies their potential application as an MRI contrast agent.
25358771	4	25	contain	have	548:551	arg1	composites					515:524	The magnetic nanoparticle-protein cage composites	476:524	The magnetic nanoparticle-protein cage composites	476:524	The magnetic nanoparticle-protein cage composites have been reported to have high magnetic relaxivity, which implies their potential application as an MRI contrast agent.
25358771	6	26	theme	MRI	966:968	arg1	relaxometry					970:980	in vitro or in vivo MRI relaxometry	946:980	in vitro or in vivo MRI relaxometry	946:980	The commonly performed techniques are field-cycling NMR relaxometry (also known as variable field relaxometry or nuclear magnetic relaxation dispersion (NMRD) profiling) and in vitro or in vivo MRI relaxometry.
25358771	4	27	theme	nanoparticle-protein	489:508	arg1	composites					515:524	The magnetic nanoparticle-protein cage composites	476:524	The magnetic nanoparticle-protein cage composites	476:524	The magnetic nanoparticle-protein cage composites have been reported to have high magnetic relaxivity, which implies their potential application as an MRI contrast agent.
25358771	5	28	theme	magnetic	651:658	arg1	relaxivity					660:669	The magnetic relaxivity	647:669	The magnetic relaxivity	647:669	The magnetic relaxivity is determined by measuring longitudinal and transverse magnetic relaxivities of the potential agent.
25358771	1	29	theme	low	180:182	arg1	relaxivity					193:202	low magnetic relaxivity	180:202	low magnetic relaxivity of currently used contrast agents	180:236	The application of magnetic resonance imaging (MRI) is often limited by low magnetic relaxivity of currently used contrast agents.
25358771	7	30	theme	MR	1130:1131	arg1	acquisition					1139:1149	in vitro MR image acquisition	1121:1149	in vitro MR image acquisition	1121:1149	Here, we describe techniques for the synthesis of nanoparticle-protein cage composite and determination of their magnetic relaxivities by in vitro MR image acquisition and data processing.
25358771	7	31	theme	relaxivities	1105:1116	arg1	relaxivities					1105:1116	their magnetic relaxivities	1090:1116	their magnetic relaxivities	1090:1116	Here, we describe techniques for the synthesis of nanoparticle-protein cage composite and determination of their magnetic relaxivities by in vitro MR image acquisition and data processing.
25358771	7	31	theme	relaxivities	1105:1116	arg1	determination					1073:1085	determination	1073:1085	determination of their magnetic relaxivities	1073:1116	Here, we describe techniques for the synthesis of nanoparticle-protein cage composite and determination of their magnetic relaxivities by in vitro MR image acquisition and data processing.
25358771	7	31	theme	relaxivities	1105:1116	arg1	composite					1059:1067	nanoparticle-protein cage composite	1033:1067	nanoparticle-protein cage composite	1033:1067	Here, we describe techniques for the synthesis of nanoparticle-protein cage composite and determination of their magnetic relaxivities by in vitro MR image acquisition and data processing.
25358771	1	32	theme	magnetic	184:191	arg1	relaxivity					193:202	low magnetic relaxivity	180:202	low magnetic relaxivity of currently used contrast agents	180:236	The application of magnetic resonance imaging (MRI) is often limited by low magnetic relaxivity of currently used contrast agents.
25358771	6	33	theme	NMR	824:826	arg1	techniques					795:804	The commonly performed techniques	772:804	The commonly performed techniques	772:804	The commonly performed techniques are field-cycling NMR relaxometry (also known as variable field relaxometry or nuclear magnetic relaxation dispersion (NMRD) profiling) and in vitro or in vivo MRI relaxometry.
25358771	6	33	theme	NMR	824:826	arg1	relaxometry					828:838	field-cycling NMR relaxometry	810:838	field-cycling NMR relaxometry (also known as variable field relaxometry or nuclear magnetic relaxation dispersion (NMRD) profiling)	810:940	The commonly performed techniques are field-cycling NMR relaxometry (also known as variable field relaxometry or nuclear magnetic relaxation dispersion (NMRD) profiling) and in vitro or in vivo MRI relaxometry.
25358771	6	34	dep	known	846:850	arg1	also					841:844	also	841:844	also	841:844	The commonly performed techniques are field-cycling NMR relaxometry (also known as variable field relaxometry or nuclear magnetic relaxation dispersion (NMRD) profiling) and in vitro or in vivo MRI relaxometry.
25358771	3	35	theme	nanoparticles	461:473	arg1	synthesis					439:447	biological synthesis	428:447	biological synthesis of magnetic nanoparticles	428:473	Protein cage has been used as a template in biological synthesis of magnetic nanoparticles.
25358771	0	36	theme	relaxivity	16:25	arg1	values					27:32	the relaxivity values	12:32	the relaxivity values of protein cage-templated nanoparticles	12:72	Determining the relaxivity values of protein cage-templated nanoparticles using magnetic resonance imaging.
25358771	4	37	theme	MRI	627:629	arg1	application					609:619	their potential application	593:619	their potential application	593:619	The magnetic nanoparticle-protein cage composites have been reported to have high magnetic relaxivity, which implies their potential application as an MRI contrast agent.
25358771	4	37	theme	MRI	627:629	arg1	agent					640:644	an MRI contrast agent	624:644	an MRI contrast agent	624:644	The magnetic nanoparticle-protein cage composites have been reported to have high magnetic relaxivity, which implies their potential application as an MRI contrast agent.
25358771	7	38	theme	nanoparticle-protein	1033:1052	arg1	relaxivities					1105:1116	their magnetic relaxivities	1090:1116	their magnetic relaxivities	1090:1116	Here, we describe techniques for the synthesis of nanoparticle-protein cage composite and determination of their magnetic relaxivities by in vitro MR image acquisition and data processing.
25358771	7	38	theme	nanoparticle-protein	1033:1052	arg1	composite					1059:1067	nanoparticle-protein cage composite	1033:1067	nanoparticle-protein cage composite	1033:1067	Here, we describe techniques for the synthesis of nanoparticle-protein cage composite and determination of their magnetic relaxivities by in vitro MR image acquisition and data processing.
25358771	2	39	theme	contrast	298:305	arg1	agents					307:312	more sensitive contrast agents	283:312	more sensitive contrast agents	283:312	This problem can be addressed by developing more sensitive contrast agents by synthesizing new types of metal complex or metallic nanoparticles.
25358771	8	40	theme	nanoparticle-protein	1312:1331	arg1	concentrations					1348:1361	different nanoparticle-protein cage composite concentrations	1302:1361	different nanoparticle-protein cage composite concentrations	1302:1361	In this method, longitudinal and transverse relaxivities are calculated by measuring relaxation rates of water hydrogen nuclei at different nanoparticle-protein cage composite concentrations.
25358771	0	41	theme	protein	37:43	arg1	nanoparticles					60:72	protein cage-templated nanoparticles	37:72	protein cage-templated nanoparticles	37:72	Determining the relaxivity values of protein cage-templated nanoparticles using magnetic resonance imaging.
25358771	7	42	theme	magnetic	1096:1103	arg1	relaxivities					1105:1116	their magnetic relaxivities	1090:1116	their magnetic relaxivities	1090:1116	Here, we describe techniques for the synthesis of nanoparticle-protein cage composite and determination of their magnetic relaxivities by in vitro MR image acquisition and data processing.
25358771	8	43	theme	relaxation	1257:1266	arg1	rates					1268:1272	relaxation rates	1257:1272	relaxation rates of water hydrogen nuclei	1257:1297	In this method, longitudinal and transverse relaxivities are calculated by measuring relaxation rates of water hydrogen nuclei at different nanoparticle-protein cage composite concentrations.
25358771	5	44	theme	potential	755:763	arg1	agent					765:769	the potential agent	751:769	the potential agent	751:769	The magnetic relaxivity is determined by measuring longitudinal and transverse magnetic relaxivities of the potential agent.
25358771	6	45	theme	dispersion	913:922	arg1	profiling					931:939	nuclear magnetic relaxation dispersion (NMRD) profiling	885:939	nuclear magnetic relaxation dispersion (NMRD) profiling	885:939	The commonly performed techniques are field-cycling NMR relaxometry (also known as variable field relaxometry or nuclear magnetic relaxation dispersion (NMRD) profiling) and in vitro or in vivo MRI relaxometry.
25358771	6	46	theme	field-cycling	810:822	arg1	techniques					795:804	The commonly performed techniques	772:804	The commonly performed techniques	772:804	The commonly performed techniques are field-cycling NMR relaxometry (also known as variable field relaxometry or nuclear magnetic relaxation dispersion (NMRD) profiling) and in vitro or in vivo MRI relaxometry.
25358771	6	46	theme	field-cycling	810:822	arg1	relaxometry					828:838	field-cycling NMR relaxometry	810:838	field-cycling NMR relaxometry (also known as variable field relaxometry or nuclear magnetic relaxation dispersion (NMRD) profiling)	810:940	The commonly performed techniques are field-cycling NMR relaxometry (also known as variable field relaxometry or nuclear magnetic relaxation dispersion (NMRD) profiling) and in vitro or in vivo MRI relaxometry.
25358771	1	47	theme	used	217:220	arg1	agents					231:236	currently used contrast agents	207:236	currently used contrast agents	207:236	The application of magnetic resonance imaging (MRI) is often limited by low magnetic relaxivity of currently used contrast agents.
25358771	0	48	theme	nanoparticles	60:72	arg1	values					27:32	the relaxivity values	12:32	the relaxivity values of protein cage-templated nanoparticles	12:72	Determining the relaxivity values of protein cage-templated nanoparticles using magnetic resonance imaging.
25358771	3	49	used	used	406:409	arg2	template					416:423	a template	414:423	a template in biological synthesis of magnetic nanoparticles	414:473	Protein cage has been used as a template in biological synthesis of magnetic nanoparticles.
25358771	3	49	used	used	406:409	arg2	cage					392:395	Protein cage	384:395	Protein cage	384:395	Protein cage has been used as a template in biological synthesis of magnetic nanoparticles.
25358771	6	50	theme	relaxation	902:911	arg1	dispersion					913:922	magnetic relaxation dispersion	893:922	nuclear magnetic relaxation dispersion (NMRD) profiling	885:939	The commonly performed techniques are field-cycling NMR relaxometry (also known as variable field relaxometry or nuclear magnetic relaxation dispersion (NMRD) profiling) and in vitro or in vivo MRI relaxometry.
25358771	6	50	theme	relaxation	902:911	arg1	NMRD					925:928	NMRD	925:928	NMRD	925:928	The commonly performed techniques are field-cycling NMR relaxometry (also known as variable field relaxometry or nuclear magnetic relaxation dispersion (NMRD) profiling) and in vitro or in vivo MRI relaxometry.
25358771	7	51	theme	composite	1059:1067	arg1	synthesis					1020:1028	the synthesis	1016:1028	the synthesis of nanoparticle-protein cage composite and determination of their magnetic relaxivities by in vitro MR image acquisition and data processing	1016:1169	Here, we describe techniques for the synthesis of nanoparticle-protein cage composite and determination of their magnetic relaxivities by in vitro MR image acquisition and data processing.
25358771	1	52	theme	magnetic	127:134	arg1	imaging					146:152	magnetic resonance imaging	127:152	magnetic resonance imaging (MRI)	127:158	The application of magnetic resonance imaging (MRI) is often limited by low magnetic relaxivity of currently used contrast agents.
25358771	1	52	theme	magnetic	127:134	arg1	MRI					155:157	MRI	155:157	MRI	155:157	The application of magnetic resonance imaging (MRI) is often limited by low magnetic relaxivity of currently used contrast agents.
25358771	0	53	theme	cage-templated	45:58	arg1	nanoparticles					60:72	protein cage-templated nanoparticles	37:72	protein cage-templated nanoparticles	37:72	Determining the relaxivity values of protein cage-templated nanoparticles using magnetic resonance imaging.
25358771	4	54	theme	magnetic	558:565	arg1	relaxivity					567:576	high magnetic relaxivity	553:576	high magnetic relaxivity	553:576	The magnetic nanoparticle-protein cage composites have been reported to have high magnetic relaxivity, which implies their potential application as an MRI contrast agent.
25358771	6	55	theme	magnetic	893:900	arg1	dispersion					913:922	magnetic relaxation dispersion	893:922	nuclear magnetic relaxation dispersion (NMRD) profiling	885:939	The commonly performed techniques are field-cycling NMR relaxometry (also known as variable field relaxometry or nuclear magnetic relaxation dispersion (NMRD) profiling) and in vitro or in vivo MRI relaxometry.
25358771	6	55	theme	magnetic	893:900	arg1	NMRD					925:928	NMRD	925:928	NMRD	925:928	The commonly performed techniques are field-cycling NMR relaxometry (also known as variable field relaxometry or nuclear magnetic relaxation dispersion (NMRD) profiling) and in vitro or in vivo MRI relaxometry.
25358771	1	56	theme	resonance	136:144	arg1	imaging					146:152	magnetic resonance imaging	127:152	magnetic resonance imaging (MRI)	127:158	The application of magnetic resonance imaging (MRI) is often limited by low magnetic relaxivity of currently used contrast agents.
25358771	1	56	theme	resonance	136:144	arg1	MRI					155:157	MRI	155:157	MRI	155:157	The application of magnetic resonance imaging (MRI) is often limited by low magnetic relaxivity of currently used contrast agents.
25358771	4	57	theme	high	553:556	arg1	relaxivity					567:576	high magnetic relaxivity	553:576	high magnetic relaxivity	553:576	The magnetic nanoparticle-protein cage composites have been reported to have high magnetic relaxivity, which implies their potential application as an MRI contrast agent.
25358771	6	58	theme	nuclear	885:891	arg1	profiling					931:939	nuclear magnetic relaxation dispersion (NMRD) profiling	885:939	nuclear magnetic relaxation dispersion (NMRD) profiling	885:939	The commonly performed techniques are field-cycling NMR relaxometry (also known as variable field relaxometry or nuclear magnetic relaxation dispersion (NMRD) profiling) and in vitro or in vivo MRI relaxometry.
25358771	6	59	theme	performed	785:793	arg1	techniques					795:804	The commonly performed techniques	772:804	The commonly performed techniques	772:804	The commonly performed techniques are field-cycling NMR relaxometry (also known as variable field relaxometry or nuclear magnetic relaxation dispersion (NMRD) profiling) and in vitro or in vivo MRI relaxometry.
25358771	6	59	theme	performed	785:793	arg1	relaxometry					828:838	field-cycling NMR relaxometry	810:838	field-cycling NMR relaxometry (also known as variable field relaxometry or nuclear magnetic relaxation dispersion (NMRD) profiling)	810:940	The commonly performed techniques are field-cycling NMR relaxometry (also known as variable field relaxometry or nuclear magnetic relaxation dispersion (NMRD) profiling) and in vitro or in vivo MRI relaxometry.
25358771	1	60	theme	imaging	146:152	arg1	application					112:122	The application	108:122	The application of magnetic resonance imaging (MRI)	108:158	The application of magnetic resonance imaging (MRI) is often limited by low magnetic relaxivity of currently used contrast agents.
25358771	1	61	theme	contrast	222:229	arg1	agents					231:236	currently used contrast agents	207:236	currently used contrast agents	207:236	The application of magnetic resonance imaging (MRI) is often limited by low magnetic relaxivity of currently used contrast agents.
25358771	5	62	theme	magnetic	726:733	arg1	relaxivities					735:746	magnetic relaxivities	726:746	magnetic relaxivities	726:746	The magnetic relaxivity is determined by measuring longitudinal and transverse magnetic relaxivities of the potential agent.
25358771	7	63	theme	cage	1054:1057	arg1	relaxivities					1105:1116	their magnetic relaxivities	1090:1116	their magnetic relaxivities	1090:1116	Here, we describe techniques for the synthesis of nanoparticle-protein cage composite and determination of their magnetic relaxivities by in vitro MR image acquisition and data processing.
25358771	7	63	theme	cage	1054:1057	arg1	composite					1059:1067	nanoparticle-protein cage composite	1033:1067	nanoparticle-protein cage composite	1033:1067	Here, we describe techniques for the synthesis of nanoparticle-protein cage composite and determination of their magnetic relaxivities by in vitro MR image acquisition and data processing.
25358771	3	64	from	template	416:423	arg1	synthesis					439:447	biological synthesis	428:447	biological synthesis of magnetic nanoparticles	428:473	Protein cage has been used as a template in biological synthesis of magnetic nanoparticles.
25358771	2	65	theme	new	330:332	arg1	types					334:338	new types	330:338	new types of metal complex or metallic nanoparticles	330:381	This problem can be addressed by developing more sensitive contrast agents by synthesizing new types of metal complex or metallic nanoparticles.
25358771	1	66	theme	agents	231:236	arg1	relaxivity					193:202	low magnetic relaxivity	180:202	low magnetic relaxivity of currently used contrast agents	180:236	The application of magnetic resonance imaging (MRI) is often limited by low magnetic relaxivity of currently used contrast agents.
25358771	0	67	theme	magnetic	80:87	arg1	imaging					99:105	magnetic resonance imaging	80:105	magnetic resonance imaging	80:105	Determining the relaxivity values of protein cage-templated nanoparticles using magnetic resonance imaging.
25358771	3	68	theme	magnetic	452:459	arg1	nanoparticles					461:473	magnetic nanoparticles	452:473	magnetic nanoparticles	452:473	Protein cage has been used as a template in biological synthesis of magnetic nanoparticles.
25358771	8	69	theme	composite	1338:1346	arg1	concentrations					1348:1361	different nanoparticle-protein cage composite concentrations	1302:1361	different nanoparticle-protein cage composite concentrations	1302:1361	In this method, longitudinal and transverse relaxivities are calculated by measuring relaxation rates of water hydrogen nuclei at different nanoparticle-protein cage composite concentrations.
25358771	8	70	theme	cage	1333:1336	arg1	concentrations					1348:1361	different nanoparticle-protein cage composite concentrations	1302:1361	different nanoparticle-protein cage composite concentrations	1302:1361	In this method, longitudinal and transverse relaxivities are calculated by measuring relaxation rates of water hydrogen nuclei at different nanoparticle-protein cage composite concentrations.
25358771	2	71	theme	nanoparticles	369:381	arg1	types					334:338	new types	330:338	new types of metal complex or metallic nanoparticles	330:381	This problem can be addressed by developing more sensitive contrast agents by synthesizing new types of metal complex or metallic nanoparticles.
25358771	8	72	theme	hydrogen	1283:1290	arg1	nuclei					1292:1297	water hydrogen nuclei	1277:1297	water hydrogen nuclei	1277:1297	In this method, longitudinal and transverse relaxivities are calculated by measuring relaxation rates of water hydrogen nuclei at different nanoparticle-protein cage composite concentrations.
25358771	8	73	theme	water	1277:1281	arg1	nuclei					1292:1297	water hydrogen nuclei	1277:1297	water hydrogen nuclei	1277:1297	In this method, longitudinal and transverse relaxivities are calculated by measuring relaxation rates of water hydrogen nuclei at different nanoparticle-protein cage composite concentrations.
25358771	6	74	theme	in	958:959	arg1	relaxometry					970:980	in vitro or in vivo MRI relaxometry	946:980	in vitro or in vivo MRI relaxometry	946:980	The commonly performed techniques are field-cycling NMR relaxometry (also known as variable field relaxometry or nuclear magnetic relaxation dispersion (NMRD) profiling) and in vitro or in vivo MRI relaxometry.
25358771	3	75	theme	Protein	384:390	arg1	cage					392:395	Protein cage	384:395	Protein cage	384:395	Protein cage has been used as a template in biological synthesis of magnetic nanoparticles.
25358771	3	75	theme	Protein	384:390	arg1	template					416:423	a template	414:423	a template in biological synthesis of magnetic nanoparticles	414:473	Protein cage has been used as a template in biological synthesis of magnetic nanoparticles.
25358771	2	76	theme	metallic	360:367	arg1	nanoparticles					369:381	metal complex or metallic nanoparticles	343:381	metal complex or metallic nanoparticles	343:381	This problem can be addressed by developing more sensitive contrast agents by synthesizing new types of metal complex or metallic nanoparticles.
25358771	4	77	theme	contrast	631:638	arg1	application					609:619	their potential application	593:619	their potential application	593:619	The magnetic nanoparticle-protein cage composites have been reported to have high magnetic relaxivity, which implies their potential application as an MRI contrast agent.
25358771	4	77	theme	contrast	631:638	arg1	agent					640:644	an MRI contrast agent	624:644	an MRI contrast agent	624:644	The magnetic nanoparticle-protein cage composites have been reported to have high magnetic relaxivity, which implies their potential application as an MRI contrast agent.
27464990	8	0	theme	LER	1125:1127	arg1	phenomenon					1129:1138	the LER phenomenon	1121:1138	the LER phenomenon	1121:1138	In conclusion, the LER phenomenon is caused by endotoxin masking and not by test interference.
27464990	6	1	theme	nonionic	876:883	arg1	surfactants					885:895	nonionic surfactants	876:895	nonionic surfactants	876:895	Furthermore, only the simultaneous presence of nonionic surfactants and components capable of forming metal complexes resulted in LER.
27464990	10	2	theme	masking	1422:1428	arg1	mechanism					1399:1407	a two-step mechanism	1388:1407	a two-step mechanism of endotoxin masking by complex forming agents and nonionic surfactants	1388:1479	We propose a two-step mechanism of endotoxin masking by complex forming agents and nonionic surfactants.
27464990	4	3	theme	endotoxin	628:636	arg1	detection					638:646	endotoxin detection	628:646	endotoxin detection in samples containing nonionic surfactants in various buffer systems	628:715	In order to analyze the driving forces of LER, endotoxin detection in samples containing nonionic surfactants in various buffer systems was investigated.
27464990	6	4	theme	simultaneous	851:862	arg1	presence					864:871	only the simultaneous presence	842:871	only the simultaneous presence of nonionic surfactants and components capable of forming metal complexes	842:945	Furthermore, only the simultaneous presence of nonionic surfactants and components capable of forming metal complexes resulted in LER.
27464990	0	5	theme	detection	69:77	arg1	systems					79:85	Limulus-based detection systems	55:85	Limulus-based detection systems	55:85	Masking of endotoxin in surfactant samples: Effects on Limulus-based detection systems.
27464990	10	6	theme	endotoxin	1412:1420	arg1	masking					1422:1428	endotoxin masking	1412:1428	endotoxin masking	1412:1428	We propose a two-step mechanism of endotoxin masking by complex forming agents and nonionic surfactants.
27464990	2	7	theme	Endotoxin	303:311	arg1	Recovery					313:320	Low Endotoxin Recovery	299:320	Low Endotoxin Recovery (LER) in biopharmaceutical drug products	299:361	Recently, Low Endotoxin Recovery (LER) in biopharmaceutical drug products has been noticed, whereby the detection of potential endotoxin contaminations is not ensured.
27464990	2	7	theme	Endotoxin	303:311	arg1	LER					323:325	LER	323:325	LER	323:325	Recently, Low Endotoxin Recovery (LER) in biopharmaceutical drug products has been noticed, whereby the detection of potential endotoxin contaminations is not ensured.
27464990	3	8	contain	have	525:528	arg1	surfactants					502:512	surfactants	502:512	surfactants	502:512	Notably, most of these drug products contain surfactants, which can have crucial effects on the detectability of endotoxin.
27464990	3	8	contain	have	525:528	arg2	effects					538:544	crucial effects	530:544	crucial effects	530:544	Notably, most of these drug products contain surfactants, which can have crucial effects on the detectability of endotoxin.
27464990	0	9	from	Masking	0:6	arg1	samples					35:41	surfactant samples	24:41	surfactant samples	24:41	Masking of endotoxin in surfactant samples: Effects on Limulus-based detection systems.
27464990	7	10	theme	formulation	1080:1090	arg1	compositions					1092:1103	such formulation compositions	1075:1103	such formulation compositions	1075:1103	In addition, capacity experiments show that even hazardous amounts of endotoxin can remain undetectable within such formulation compositions.
27464990	0	11	from	Effects	44:50	arg1	systems					79:85	Limulus-based detection systems	55:85	Limulus-based detection systems	55:85	Masking of endotoxin in surfactant samples: Effects on Limulus-based detection systems.
27464990	6	12	theme	components	901:910	arg1	presence					864:871	only the simultaneous presence	842:871	only the simultaneous presence of nonionic surfactants and components capable of forming metal complexes	842:945	Furthermore, only the simultaneous presence of nonionic surfactants and components capable of forming metal complexes resulted in LER.
27464990	4	13	theme	buffer	702:707	arg1	systems					709:715	various buffer systems	694:715	various buffer systems	694:715	In order to analyze the driving forces of LER, endotoxin detection in samples containing nonionic surfactants in various buffer systems was investigated.
27464990	10	14	theme	two-step	1390:1397	arg1	mechanism					1399:1407	a two-step mechanism	1388:1407	a two-step mechanism of endotoxin masking by complex forming agents and nonionic surfactants	1388:1479	We propose a two-step mechanism of endotoxin masking by complex forming agents and nonionic surfactants.
27464990	2	15	theme	Low	299:301	arg1	Recovery					313:320	Low Endotoxin Recovery	299:320	Low Endotoxin Recovery (LER) in biopharmaceutical drug products	299:361	Recently, Low Endotoxin Recovery (LER) in biopharmaceutical drug products has been noticed, whereby the detection of potential endotoxin contaminations is not ensured.
27464990	2	15	theme	Low	299:301	arg1	LER					323:325	LER	323:325	LER	323:325	Recently, Low Endotoxin Recovery (LER) in biopharmaceutical drug products has been noticed, whereby the detection of potential endotoxin contaminations is not ensured.
27464990	2	16	theme	drug	349:352	arg1	products					354:361	biopharmaceutical drug products	331:361	biopharmaceutical drug products	331:361	Recently, Low Endotoxin Recovery (LER) in biopharmaceutical drug products has been noticed, whereby the detection of potential endotoxin contaminations is not ensured.
27464990	7	17	theme	such	1075:1078	arg1	compositions					1092:1103	such formulation compositions	1075:1103	such formulation compositions	1075:1103	In addition, capacity experiments show that even hazardous amounts of endotoxin can remain undetectable within such formulation compositions.
27464990	2	18	from	Recovery	313:320	arg1	products					354:361	biopharmaceutical drug products	331:361	biopharmaceutical drug products	331:361	Recently, Low Endotoxin Recovery (LER) in biopharmaceutical drug products has been noticed, whereby the detection of potential endotoxin contaminations is not ensured.
27464990	3	19	theme	drug	480:483	arg1	products					485:492	these drug products	474:492	these drug products	474:492	Notably, most of these drug products contain surfactants, which can have crucial effects on the detectability of endotoxin.
27464990	2	20	theme	biopharmaceutical	331:347	arg1	products					354:361	biopharmaceutical drug products	331:361	biopharmaceutical drug products	331:361	Recently, Low Endotoxin Recovery (LER) in biopharmaceutical drug products has been noticed, whereby the detection of potential endotoxin contaminations is not ensured.
27464990	9	21	theme	limited	1291:1297	arg1	susceptibility					1299:1312	only a limited susceptibility	1284:1312	only a limited susceptibility	1284:1312	In this process, the supramolecular structure of endotoxin is altered and exhibits only a limited susceptibility in binding to the Factor C of Limulus-based detection systems.
27464990	10	22	theme	complex	1433:1439	arg1	agents					1449:1454	complex forming agents	1433:1454	complex forming agents	1433:1454	We propose a two-step mechanism of endotoxin masking by complex forming agents and nonionic surfactants.
27464990	4	23	contain	containing	659:668	arg1	samples					651:657	samples	651:657	samples containing nonionic surfactants in various buffer systems	651:715	In order to analyze the driving forces of LER, endotoxin detection in samples containing nonionic surfactants in various buffer systems was investigated.
27464990	4	23	contain	containing	659:668	arg2	surfactants					679:689	nonionic surfactants	670:689	nonionic surfactants	670:689	In order to analyze the driving forces of LER, endotoxin detection in samples containing nonionic surfactants in various buffer systems was investigated.
27464990	8	24	theme	endotoxin	1153:1161	arg1	masking					1163:1169	endotoxin masking	1153:1169	endotoxin masking	1153:1169	In conclusion, the LER phenomenon is caused by endotoxin masking and not by test interference.
27464990	4	25	theme	nonionic	670:677	arg1	surfactants					679:689	nonionic surfactants	670:689	nonionic surfactants	670:689	In order to analyze the driving forces of LER, endotoxin detection in samples containing nonionic surfactants in various buffer systems was investigated.
27464990	4	26	theme	driving	605:611	arg1	forces					613:618	the driving forces	601:618	the driving forces of LER	601:625	In order to analyze the driving forces of LER, endotoxin detection in samples containing nonionic surfactants in various buffer systems was investigated.
27464990	7	27	theme	endotoxin	1034:1042	arg1	endotoxin					1034:1042	endotoxin	1034:1042	endotoxin	1034:1042	In addition, capacity experiments show that even hazardous amounts of endotoxin can remain undetectable within such formulation compositions.
27464990	7	27	theme	endotoxin	1034:1042	arg1	amounts					1023:1029	even hazardous amounts	1008:1029	even hazardous amounts of endotoxin	1008:1042	In addition, capacity experiments show that even hazardous amounts of endotoxin can remain undetectable within such formulation compositions.
27464990	4	28	theme	various	694:700	arg1	systems					709:715	various buffer systems	694:715	various buffer systems	694:715	In order to analyze the driving forces of LER, endotoxin detection in samples containing nonionic surfactants in various buffer systems was investigated.
27464990	1	29	theme	sensitive	163:171	arg1	method					173:178	the most sensitive method	154:178	the most sensitive method for the detection of endotoxins (Lipopolysaccharides)	154:232	Over the last few decades Limulus Amebocyte Lysate (LAL) has been the most sensitive method for the detection of endotoxins (Lipopolysaccharides) and is well accepted in a broad field of applications.
27464990	1	29	theme	sensitive	163:171	arg1	Lysate					132:137	Limulus Amebocyte Lysate	114:137	Limulus Amebocyte Lysate (LAL)	114:143	Over the last few decades Limulus Amebocyte Lysate (LAL) has been the most sensitive method for the detection of endotoxins (Lipopolysaccharides) and is well accepted in a broad field of applications.
27464990	0	30	theme	endotoxin	11:19	arg1	Masking					0:6	Masking	0:6	Masking of endotoxin in surfactant samples: Effects on Limulus-based detection systems.	0:86	Masking of endotoxin in surfactant samples: Effects on Limulus-based detection systems.
27464990	6	31	theme	metal	931:935	arg1	complexes					937:945	metal complexes	931:945	metal complexes	931:945	Furthermore, only the simultaneous presence of nonionic surfactants and components capable of forming metal complexes resulted in LER.
27464990	1	32	theme	last	97:100	arg1	decades					106:112	the last few decades	93:112	the last few decades	93:112	Over the last few decades Limulus Amebocyte Lysate (LAL) has been the most sensitive method for the detection of endotoxins (Lipopolysaccharides) and is well accepted in a broad field of applications.
27464990	9	33	theme	endotoxin	1250:1258	arg1	structure					1237:1245	the supramolecular structure	1218:1245	the supramolecular structure of endotoxin	1218:1258	In this process, the supramolecular structure of endotoxin is altered and exhibits only a limited susceptibility in binding to the Factor C of Limulus-based detection systems.
27464990	2	34	theme	contaminations	426:439	arg1	detection					393:401	the detection	389:401	the detection of potential endotoxin contaminations	389:439	Recently, Low Endotoxin Recovery (LER) in biopharmaceutical drug products has been noticed, whereby the detection of potential endotoxin contaminations is not ensured.
27464990	10	35	theme	nonionic	1460:1467	arg1	surfactants					1469:1479	nonionic surfactants	1460:1479	nonionic surfactants	1460:1479	We propose a two-step mechanism of endotoxin masking by complex forming agents and nonionic surfactants.
27464990	2	36	theme	endotoxin	416:424	arg1	contaminations					426:439	potential endotoxin contaminations	406:439	potential endotoxin contaminations	406:439	Recently, Low Endotoxin Recovery (LER) in biopharmaceutical drug products has been noticed, whereby the detection of potential endotoxin contaminations is not ensured.
27464990	1	37	theme	broad	260:264	arg1	field					266:270	a broad field	258:270	a broad field of applications	258:286	Over the last few decades Limulus Amebocyte Lysate (LAL) has been the most sensitive method for the detection of endotoxins (Lipopolysaccharides) and is well accepted in a broad field of applications.
27464990	0	38	theme	surfactant	24:33	arg1	samples					35:41	surfactant samples	24:41	surfactant samples	24:41	Masking of endotoxin in surfactant samples: Effects on Limulus-based detection systems.
27464990	9	39	theme	systems	1368:1374	arg1	C					1339:1339	the Factor C	1328:1339	the Factor C of Limulus-based detection systems	1328:1374	In this process, the supramolecular structure of endotoxin is altered and exhibits only a limited susceptibility in binding to the Factor C of Limulus-based detection systems.
27464990	7	40	theme	capacity	977:984	arg1	experiments					986:996	capacity experiments	977:996	capacity experiments	977:996	In addition, capacity experiments show that even hazardous amounts of endotoxin can remain undetectable within such formulation compositions.
27464990	10	41	theme	forming	1441:1447	arg1	agents					1449:1454	complex forming agents	1433:1454	complex forming agents	1433:1454	We propose a two-step mechanism of endotoxin masking by complex forming agents and nonionic surfactants.
27464990	2	42	theme	potential	406:414	arg1	contaminations					426:439	potential endotoxin contaminations	406:439	potential endotoxin contaminations	406:439	Recently, Low Endotoxin Recovery (LER) in biopharmaceutical drug products has been noticed, whereby the detection of potential endotoxin contaminations is not ensured.
27464990	6	43	theme	capable	912:918	arg1	surfactants					885:895	nonionic surfactants	876:895	nonionic surfactants	876:895	Furthermore, only the simultaneous presence of nonionic surfactants and components capable of forming metal complexes resulted in LER.
27464990	1	44	theme	few	102:104	arg1	decades					106:112	the last few decades	93:112	the last few decades	93:112	Over the last few decades Limulus Amebocyte Lysate (LAL) has been the most sensitive method for the detection of endotoxins (Lipopolysaccharides) and is well accepted in a broad field of applications.
27464990	3	45	contain	contain	494:500	arg1	products					485:492	these drug products	474:492	these drug products	474:492	Notably, most of these drug products contain surfactants, which can have crucial effects on the detectability of endotoxin.
27464990	3	45	contain	contain	494:500	arg1	most					466:469	most	466:469	most	466:469	Notably, most of these drug products contain surfactants, which can have crucial effects on the detectability of endotoxin.
27464990	3	45	contain	contain	494:500	arg2	surfactants					502:512	surfactants	502:512	surfactants	502:512	Notably, most of these drug products contain surfactants, which can have crucial effects on the detectability of endotoxin.
27464990	7	46	theme	hazardous	1013:1021	arg1	endotoxin					1034:1042	endotoxin	1034:1042	endotoxin	1034:1042	In addition, capacity experiments show that even hazardous amounts of endotoxin can remain undetectable within such formulation compositions.
27464990	7	46	theme	hazardous	1013:1021	arg1	amounts					1023:1029	even hazardous amounts	1008:1029	even hazardous amounts of endotoxin	1008:1042	In addition, capacity experiments show that even hazardous amounts of endotoxin can remain undetectable within such formulation compositions.
27464990	3	47	theme	crucial	530:536	arg1	effects					538:544	crucial effects	530:544	crucial effects	530:544	Notably, most of these drug products contain surfactants, which can have crucial effects on the detectability of endotoxin.
27464990	8	48	theme	test	1182:1185	arg1	interference					1187:1198	test interference	1182:1198	test interference	1182:1198	In conclusion, the LER phenomenon is caused by endotoxin masking and not by test interference.
27464990	1	49	theme	applications	275:286	arg1	field					266:270	a broad field	258:270	a broad field of applications	258:286	Over the last few decades Limulus Amebocyte Lysate (LAL) has been the most sensitive method for the detection of endotoxins (Lipopolysaccharides) and is well accepted in a broad field of applications.
27464990	9	50	theme	supramolecular	1222:1235	arg1	structure					1237:1245	the supramolecular structure	1218:1245	the supramolecular structure of endotoxin	1218:1258	In this process, the supramolecular structure of endotoxin is altered and exhibits only a limited susceptibility in binding to the Factor C of Limulus-based detection systems.
27464990	1	51	theme	Limulus	114:120	arg1	method					173:178	the most sensitive method	154:178	the most sensitive method for the detection of endotoxins (Lipopolysaccharides)	154:232	Over the last few decades Limulus Amebocyte Lysate (LAL) has been the most sensitive method for the detection of endotoxins (Lipopolysaccharides) and is well accepted in a broad field of applications.
27464990	1	51	theme	Limulus	114:120	arg1	LAL					140:142	LAL	140:142	LAL	140:142	Over the last few decades Limulus Amebocyte Lysate (LAL) has been the most sensitive method for the detection of endotoxins (Lipopolysaccharides) and is well accepted in a broad field of applications.
27464990	1	51	theme	Limulus	114:120	arg1	Lysate					132:137	Limulus Amebocyte Lysate	114:137	Limulus Amebocyte Lysate (LAL)	114:143	Over the last few decades Limulus Amebocyte Lysate (LAL) has been the most sensitive method for the detection of endotoxins (Lipopolysaccharides) and is well accepted in a broad field of applications.
27464990	9	52	theme	Limulus-based	1344:1356	arg1	systems					1368:1374	Limulus-based detection systems	1344:1374	Limulus-based detection systems	1344:1374	In this process, the supramolecular structure of endotoxin is altered and exhibits only a limited susceptibility in binding to the Factor C of Limulus-based detection systems.
27464990	3	53	theme	endotoxin	570:578	arg1	detectability					553:565	the detectability	549:565	the detectability of endotoxin	549:578	Notably, most of these drug products contain surfactants, which can have crucial effects on the detectability of endotoxin.
27464990	1	54	theme	Amebocyte	122:130	arg1	method					173:178	the most sensitive method	154:178	the most sensitive method for the detection of endotoxins (Lipopolysaccharides)	154:232	Over the last few decades Limulus Amebocyte Lysate (LAL) has been the most sensitive method for the detection of endotoxins (Lipopolysaccharides) and is well accepted in a broad field of applications.
27464990	1	54	theme	Amebocyte	122:130	arg1	LAL					140:142	LAL	140:142	LAL	140:142	Over the last few decades Limulus Amebocyte Lysate (LAL) has been the most sensitive method for the detection of endotoxins (Lipopolysaccharides) and is well accepted in a broad field of applications.
27464990	1	54	theme	Amebocyte	122:130	arg1	Lysate					132:137	Limulus Amebocyte Lysate	114:137	Limulus Amebocyte Lysate (LAL)	114:143	Over the last few decades Limulus Amebocyte Lysate (LAL) has been the most sensitive method for the detection of endotoxins (Lipopolysaccharides) and is well accepted in a broad field of applications.
27464990	9	55	theme	detection	1358:1366	arg1	systems					1368:1374	Limulus-based detection systems	1344:1374	Limulus-based detection systems	1344:1374	In this process, the supramolecular structure of endotoxin is altered and exhibits only a limited susceptibility in binding to the Factor C of Limulus-based detection systems.
27464990	0	56	theme	Limulus-based	55:67	arg1	systems					79:85	Limulus-based detection systems	55:85	Limulus-based detection systems	55:85	Masking of endotoxin in surfactant samples: Effects on Limulus-based detection systems.
27464990	5	57	theme	LER	772:774	arg1	controlled					791:800	controlled	791:800	controlled	791:800	The results show that the process of LER is kinetically controlled and temperature-dependent.
27464990	5	57	theme	LER	772:774	arg1	process					761:767	the process	757:767	the process of LER	757:774	The results show that the process of LER is kinetically controlled and temperature-dependent.
27464990	4	58	theme	LER	623:625	arg1	forces					613:618	the driving forces	601:618	the driving forces of LER	601:625	In order to analyze the driving forces of LER, endotoxin detection in samples containing nonionic surfactants in various buffer systems was investigated.
27464990	4	59	from	detection	638:646	arg1	samples					651:657	samples	651:657	samples containing nonionic surfactants in various buffer systems	651:715	In order to analyze the driving forces of LER, endotoxin detection in samples containing nonionic surfactants in various buffer systems was investigated.
27464990	1	60	theme	endotoxins	201:210	arg1	detection					188:196	the detection	184:196	the detection of endotoxins (Lipopolysaccharides)	184:232	Over the last few decades Limulus Amebocyte Lysate (LAL) has been the most sensitive method for the detection of endotoxins (Lipopolysaccharides) and is well accepted in a broad field of applications.
27464990	6	61	theme	surfactants	885:895	arg1	presence					864:871	only the simultaneous presence	842:871	only the simultaneous presence of nonionic surfactants and components capable of forming metal complexes	842:945	Furthermore, only the simultaneous presence of nonionic surfactants and components capable of forming metal complexes resulted in LER.
27464990	0	62	dep	Masking	0:6	arg1	Effects					44:50	Effects	44:50	Masking of endotoxin in surfactant samples: Effects on Limulus-based detection systems.	0:86	Masking of endotoxin in surfactant samples: Effects on Limulus-based detection systems.
27464990	9	63	theme	Factor	1332:1337	arg1	C					1339:1339	the Factor C	1328:1339	the Factor C of Limulus-based detection systems	1328:1374	In this process, the supramolecular structure of endotoxin is altered and exhibits only a limited susceptibility in binding to the Factor C of Limulus-based detection systems.
27270599	0	0	theme	microbial	63:71	arg1	community					73:81	the microbial community	59:81	the microbial community	59:81	Impact of sludge retention time on the fine composition of the microbial community and extracellular polymeric substances in a membrane bioreactor.
27270599	6	1	theme	microbial	778:786	arg1	structure					798:806	the microbial community structure	774:806	the microbial community structure	774:806	A comprehensive analysis of the microbial community structure and EPS proteins and polysaccharide profiles of the mixed liquor and cake layer was carried out throughout both operation periods.
27270599	1	2	theme	Membrane	148:155	arg1	technology					192:201	an advanced technology	180:201	an advanced technology for wastewater treatment whose wide application has been hindered by rapid fouling of the membranes	180:301	Membrane bioreactors (MBRs) are an advanced technology for wastewater treatment whose wide application has been hindered by rapid fouling of the membranes.
27270599	1	2	theme	Membrane	148:155	arg1	MBRs					170:173	MBRs	170:173	MBRs	170:173	Membrane bioreactors (MBRs) are an advanced technology for wastewater treatment whose wide application has been hindered by rapid fouling of the membranes.
27270599	1	2	theme	Membrane	148:155	arg1	bioreactors					157:167	Membrane bioreactors	148:167	Membrane bioreactors (MBRs)	148:174	Membrane bioreactors (MBRs) are an advanced technology for wastewater treatment whose wide application has been hindered by rapid fouling of the membranes.
27270599	8	3	theme	cake	1084:1087	arg1	communities					1095:1105	The mixed liquor and cake layer communities	1063:1105	The mixed liquor and cake layer communities	1063:1105	The mixed liquor and cake layer communities were very different, with Actinomycetales order standing out in the cake layer at SRT of 20 days.
27270599	10	4	theme	polysaccharide	1485:1498	arg1	monomers					1506:1513	polysaccharide sugar monomers	1485:1513	polysaccharide sugar monomers	1485:1513	Furthermore, EPS profiles were clearly affected by the SRT: it was possible to correlate a group of soluble EPS proteins with the SRT of 60 days, and a lower sludge age led to a lower diversity of polysaccharide sugar monomers, with an increase of glucose and galactose in the cake layer.
27270599	2	5	theme	long	330:333	arg1	parameter					374:382	a crucial parameter	364:382	a crucial parameter impacting microbial selection in the reactor	364:427	MBRs can be operated with long sludge retention time (SRT), a crucial parameter impacting microbial selection in the reactor.
27270599	2	5	theme	long	330:333	arg1	SRT					358:360	SRT	358:360	SRT	358:360	MBRs can be operated with long sludge retention time (SRT), a crucial parameter impacting microbial selection in the reactor.
27270599	2	5	theme	long	330:333	arg1	time					352:355	long sludge retention time	330:355	long sludge retention time (SRT)	330:361	MBRs can be operated with long sludge retention time (SRT), a crucial parameter impacting microbial selection in the reactor.
27270599	10	6	theme	proteins	1400:1407	arg1	group					1379:1383	a group	1377:1383	a group of soluble EPS proteins	1377:1407	Furthermore, EPS profiles were clearly affected by the SRT: it was possible to correlate a group of soluble EPS proteins with the SRT of 60 days, and a lower sludge age led to a lower diversity of polysaccharide sugar monomers, with an increase of glucose and galactose in the cake layer.
27270599	10	6	theme	proteins	1400:1407	arg1	proteins					1400:1407	soluble EPS proteins	1388:1407	soluble EPS proteins	1388:1407	Furthermore, EPS profiles were clearly affected by the SRT: it was possible to correlate a group of soluble EPS proteins with the SRT of 60 days, and a lower sludge age led to a lower diversity of polysaccharide sugar monomers, with an increase of glucose and galactose in the cake layer.
27270599	0	7	from	Impact	0:5	arg1	substances					111:120	extracellular polymeric substances	87:120	extracellular polymeric substances in a membrane bioreactor	87:145	Impact of sludge retention time on the fine composition of the microbial community and extracellular polymeric substances in a membrane bioreactor.
27270599	0	7	from	Impact	0:5	arg1	composition					44:54	the fine composition	35:54	the fine composition of the microbial community	35:81	Impact of sludge retention time on the fine composition of the microbial community and extracellular polymeric substances in a membrane bioreactor.
27270599	7	8	theme	SRT	1005:1007	arg1	imposition					981:990	the imposition	977:990	the imposition of a shorter SRT	977:1007	The results of this study showed that the imposition of a shorter SRT led to a shift in the dominant bacterial populations.
27270599	10	9	theme	cake	1565:1568	arg1	layer					1570:1574	the cake layer	1561:1574	the cake layer	1561:1574	Furthermore, EPS profiles were clearly affected by the SRT: it was possible to correlate a group of soluble EPS proteins with the SRT of 60 days, and a lower sludge age led to a lower diversity of polysaccharide sugar monomers, with an increase of glucose and galactose in the cake layer.
27270599	8	10	dep	different	1117:1125	arg1	standing					1155:1162	standing	1155:1162	standing out in the cake layer at SRT of 20 days	1155:1202	The mixed liquor and cake layer communities were very different, with Actinomycetales order standing out in the cake layer at SRT of 20 days.
27270599	0	11	theme	community	73:81	arg1	composition					44:54	the fine composition	35:54	the fine composition of the microbial community	35:81	Impact of sludge retention time on the fine composition of the microbial community and extracellular polymeric substances in a membrane bioreactor.
27270599	0	11	theme	community	73:81	arg1	substances					111:120	extracellular polymeric substances	87:120	extracellular polymeric substances in a membrane bioreactor	87:145	Impact of sludge retention time on the fine composition of the microbial community and extracellular polymeric substances in a membrane bioreactor.
27270599	10	12	theme	glucose	1536:1542	arg1	increase					1524:1531	an increase	1521:1531	an increase of glucose and galactose in the cake layer	1521:1574	Furthermore, EPS profiles were clearly affected by the SRT: it was possible to correlate a group of soluble EPS proteins with the SRT of 60 days, and a lower sludge age led to a lower diversity of polysaccharide sugar monomers, with an increase of glucose and galactose in the cake layer.
27270599	3	13	theme	extracellular	508:520	arg1	substances					532:541	the extracellular polymeric substances	504:541	the extracellular polymeric substances (EPS)	504:547	This also affects filtration performance, since a major fouling agent are the extracellular polymeric substances (EPS).
27270599	3	13	theme	extracellular	508:520	arg1	agent					494:498	a major fouling agent	478:498	a major fouling agent	478:498	This also affects filtration performance, since a major fouling agent are the extracellular polymeric substances (EPS).
27270599	3	13	theme	extracellular	508:520	arg1	EPS					544:546	EPS	544:546	EPS	544:546	This also affects filtration performance, since a major fouling agent are the extracellular polymeric substances (EPS).
27270599	10	14	theme	lower	1440:1444	arg1	age					1453:1455	a lower sludge age	1438:1455	a lower sludge age	1438:1455	Furthermore, EPS profiles were clearly affected by the SRT: it was possible to correlate a group of soluble EPS proteins with the SRT of 60 days, and a lower sludge age led to a lower diversity of polysaccharide sugar monomers, with an increase of glucose and galactose in the cake layer.
27270599	2	15	theme	sludge	335:340	arg1	parameter					374:382	a crucial parameter	364:382	a crucial parameter impacting microbial selection in the reactor	364:427	MBRs can be operated with long sludge retention time (SRT), a crucial parameter impacting microbial selection in the reactor.
27270599	2	15	theme	sludge	335:340	arg1	SRT					358:360	SRT	358:360	SRT	358:360	MBRs can be operated with long sludge retention time (SRT), a crucial parameter impacting microbial selection in the reactor.
27270599	2	15	theme	sludge	335:340	arg1	time					352:355	long sludge retention time	330:355	long sludge retention time (SRT)	330:361	MBRs can be operated with long sludge retention time (SRT), a crucial parameter impacting microbial selection in the reactor.
27270599	4	16	theme	membrane	642:649	arg1	fouling					651:657	membrane fouling	642:657	membrane fouling	642:657	In this study, the impact of the SRT on the ecophysiology of the MBRs and, consequently, on membrane fouling was evaluated.
27270599	0	17	theme	polymeric	101:109	arg1	substances					111:120	extracellular polymeric substances	87:120	extracellular polymeric substances in a membrane bioreactor	87:145	Impact of sludge retention time on the fine composition of the microbial community and extracellular polymeric substances in a membrane bioreactor.
27270599	6	18	theme	structure	798:806	arg1	analysis					762:769	A comprehensive analysis	746:769	A comprehensive analysis of the microbial community structure	746:806	A comprehensive analysis of the microbial community structure and EPS proteins and polysaccharide profiles of the mixed liquor and cake layer was carried out throughout both operation periods.
27270599	6	18	theme	structure	798:806	arg1	profiles					844:851	polysaccharide profiles	829:851	polysaccharide profiles	829:851	A comprehensive analysis of the microbial community structure and EPS proteins and polysaccharide profiles of the mixed liquor and cake layer was carried out throughout both operation periods.
27270599	6	18	theme	structure	798:806	arg1	proteins					816:823	EPS proteins	812:823	EPS proteins	812:823	A comprehensive analysis of the microbial community structure and EPS proteins and polysaccharide profiles of the mixed liquor and cake layer was carried out throughout both operation periods.
27270599	5	19	theme	20 days	737:743	arg1	SRT					730:732	a SRT	728:732	a SRT of 20 days	728:743	A MBR was operated under a SRT of 60 days followed by a SRT of 20 days.
27270599	10	20	theme	lower	1466:1470	arg1	diversity					1472:1480	a lower diversity	1464:1480	a lower diversity of polysaccharide sugar monomers	1464:1513	Furthermore, EPS profiles were clearly affected by the SRT: it was possible to correlate a group of soluble EPS proteins with the SRT of 60 days, and a lower sludge age led to a lower diversity of polysaccharide sugar monomers, with an increase of glucose and galactose in the cake layer.
27270599	0	21	theme	extracellular	87:99	arg1	substances					111:120	extracellular polymeric substances	87:120	extracellular polymeric substances in a membrane bioreactor	87:145	Impact of sludge retention time on the fine composition of the microbial community and extracellular polymeric substances in a membrane bioreactor.
27270599	6	22	theme	community	788:796	arg1	structure					798:806	the microbial community structure	774:806	the microbial community structure	774:806	A comprehensive analysis of the microbial community structure and EPS proteins and polysaccharide profiles of the mixed liquor and cake layer was carried out throughout both operation periods.
27270599	6	23	theme	cake	877:880	arg1	layer					882:886	cake layer	877:886	cake layer	877:886	A comprehensive analysis of the microbial community structure and EPS proteins and polysaccharide profiles of the mixed liquor and cake layer was carried out throughout both operation periods.
27270599	6	24	theme	comprehensive	748:760	arg1	analysis					762:769	A comprehensive analysis	746:769	A comprehensive analysis of the microbial community structure	746:806	A comprehensive analysis of the microbial community structure and EPS proteins and polysaccharide profiles of the mixed liquor and cake layer was carried out throughout both operation periods.
27270599	8	25	theme	mixed	1067:1071	arg1	liquor					1073:1078	The mixed liquor	1063:1078	The mixed liquor	1063:1078	The mixed liquor and cake layer communities were very different, with Actinomycetales order standing out in the cake layer at SRT of 20 days.
27270599	8	26	theme	liquor	1073:1078	arg1	communities					1095:1105	The mixed liquor and cake layer communities	1063:1105	The mixed liquor and cake layer communities	1063:1105	The mixed liquor and cake layer communities were very different, with Actinomycetales order standing out in the cake layer at SRT of 20 days.
27270599	3	27	theme	fouling	486:492	arg1	substances					532:541	the extracellular polymeric substances	504:541	the extracellular polymeric substances (EPS)	504:547	This also affects filtration performance, since a major fouling agent are the extracellular polymeric substances (EPS).
27270599	3	27	theme	fouling	486:492	arg1	agent					494:498	a major fouling agent	478:498	a major fouling agent	478:498	This also affects filtration performance, since a major fouling agent are the extracellular polymeric substances (EPS).
27270599	10	28	from	increase	1524:1531	arg1	layer					1570:1574	the cake layer	1561:1574	the cake layer	1561:1574	Furthermore, EPS profiles were clearly affected by the SRT: it was possible to correlate a group of soluble EPS proteins with the SRT of 60 days, and a lower sludge age led to a lower diversity of polysaccharide sugar monomers, with an increase of glucose and galactose in the cake layer.
27270599	10	29	theme	60 days	1425:1431	arg1	SRT					1418:1420	the SRT	1414:1420	the SRT of 60 days	1414:1431	Furthermore, EPS profiles were clearly affected by the SRT: it was possible to correlate a group of soluble EPS proteins with the SRT of 60 days, and a lower sludge age led to a lower diversity of polysaccharide sugar monomers, with an increase of glucose and galactose in the cake layer.
27270599	7	30	theme	study	959:963	arg1	results					943:949	The results	939:949	The results of this study	939:963	The results of this study showed that the imposition of a shorter SRT led to a shift in the dominant bacterial populations.
27270599	6	31	theme	layer	882:886	arg1	analysis					762:769	A comprehensive analysis	746:769	A comprehensive analysis of the microbial community structure	746:806	A comprehensive analysis of the microbial community structure and EPS proteins and polysaccharide profiles of the mixed liquor and cake layer was carried out throughout both operation periods.
27270599	6	31	theme	layer	882:886	arg1	profiles					844:851	polysaccharide profiles	829:851	polysaccharide profiles	829:851	A comprehensive analysis of the microbial community structure and EPS proteins and polysaccharide profiles of the mixed liquor and cake layer was carried out throughout both operation periods.
27270599	6	31	theme	layer	882:886	arg1	proteins					816:823	EPS proteins	812:823	EPS proteins	812:823	A comprehensive analysis of the microbial community structure and EPS proteins and polysaccharide profiles of the mixed liquor and cake layer was carried out throughout both operation periods.
27270599	10	32	theme	monomers	1506:1513	arg1	diversity					1472:1480	a lower diversity	1464:1480	a lower diversity of polysaccharide sugar monomers	1464:1513	Furthermore, EPS profiles were clearly affected by the SRT: it was possible to correlate a group of soluble EPS proteins with the SRT of 60 days, and a lower sludge age led to a lower diversity of polysaccharide sugar monomers, with an increase of glucose and galactose in the cake layer.
27270599	0	33	theme	retention	17:25	arg1	time					27:30	sludge retention time	10:30	sludge retention time	10:30	Impact of sludge retention time on the fine composition of the microbial community and extracellular polymeric substances in a membrane bioreactor.
27270599	1	34	theme	rapid	272:276	arg1	fouling					278:284	rapid fouling	272:284	rapid fouling of the membranes	272:301	Membrane bioreactors (MBRs) are an advanced technology for wastewater treatment whose wide application has been hindered by rapid fouling of the membranes.
27270599	9	35	located	found	1269:1273	arg1	SRT					1283:1285	this SRT	1278:1285	this SRT	1278:1285	Overall, higher EPS concentrations (particularly proteins) were found at this SRT.
27270599	9	35	located	found	1269:1273	arg2	concentrations					1225:1238	higher EPS concentrations	1214:1238	higher EPS concentrations (particularly proteins)	1214:1262	Overall, higher EPS concentrations (particularly proteins) were found at this SRT.
27270599	3	36	theme	polymeric	522:530	arg1	substances					532:541	the extracellular polymeric substances	504:541	the extracellular polymeric substances (EPS)	504:547	This also affects filtration performance, since a major fouling agent are the extracellular polymeric substances (EPS).
27270599	3	36	theme	polymeric	522:530	arg1	agent					494:498	a major fouling agent	478:498	a major fouling agent	478:498	This also affects filtration performance, since a major fouling agent are the extracellular polymeric substances (EPS).
27270599	3	36	theme	polymeric	522:530	arg1	EPS					544:546	EPS	544:546	EPS	544:546	This also affects filtration performance, since a major fouling agent are the extracellular polymeric substances (EPS).
27270599	6	37	theme	EPS	812:814	arg1	proteins					816:823	EPS proteins	812:823	EPS proteins	812:823	A comprehensive analysis of the microbial community structure and EPS proteins and polysaccharide profiles of the mixed liquor and cake layer was carried out throughout both operation periods.
27270599	0	38	theme	sludge	10:15	arg1	time					27:30	sludge retention time	10:30	sludge retention time	10:30	Impact of sludge retention time on the fine composition of the microbial community and extracellular polymeric substances in a membrane bioreactor.
27270599	9	39	theme	higher	1214:1219	arg1	concentrations					1225:1238	higher EPS concentrations	1214:1238	higher EPS concentrations (particularly proteins)	1214:1262	Overall, higher EPS concentrations (particularly proteins) were found at this SRT.
27270599	8	40	theme	20 days	1196:1202	arg1	SRT					1189:1191	SRT	1189:1191	SRT of 20 days	1189:1202	The mixed liquor and cake layer communities were very different, with Actinomycetales order standing out in the cake layer at SRT of 20 days.
27270599	1	41	theme	advanced	183:190	arg1	technology					192:201	an advanced technology	180:201	an advanced technology for wastewater treatment whose wide application has been hindered by rapid fouling of the membranes	180:301	Membrane bioreactors (MBRs) are an advanced technology for wastewater treatment whose wide application has been hindered by rapid fouling of the membranes.
27270599	1	41	theme	advanced	183:190	arg1	bioreactors					157:167	Membrane bioreactors	148:167	Membrane bioreactors (MBRs)	148:174	Membrane bioreactors (MBRs) are an advanced technology for wastewater treatment whose wide application has been hindered by rapid fouling of the membranes.
27270599	6	42	theme	liquor	866:871	arg1	analysis					762:769	A comprehensive analysis	746:769	A comprehensive analysis of the microbial community structure	746:806	A comprehensive analysis of the microbial community structure and EPS proteins and polysaccharide profiles of the mixed liquor and cake layer was carried out throughout both operation periods.
27270599	6	42	theme	liquor	866:871	arg1	profiles					844:851	polysaccharide profiles	829:851	polysaccharide profiles	829:851	A comprehensive analysis of the microbial community structure and EPS proteins and polysaccharide profiles of the mixed liquor and cake layer was carried out throughout both operation periods.
27270599	6	42	theme	liquor	866:871	arg1	proteins					816:823	EPS proteins	812:823	EPS proteins	812:823	A comprehensive analysis of the microbial community structure and EPS proteins and polysaccharide profiles of the mixed liquor and cake layer was carried out throughout both operation periods.
27270599	0	43	theme	membrane	127:134	arg1	bioreactor					136:145	a membrane bioreactor	125:145	a membrane bioreactor	125:145	Impact of sludge retention time on the fine composition of the microbial community and extracellular polymeric substances in a membrane bioreactor.
27270599	10	44	theme	sugar	1500:1504	arg1	monomers					1506:1513	polysaccharide sugar monomers	1485:1513	polysaccharide sugar monomers	1485:1513	Furthermore, EPS profiles were clearly affected by the SRT: it was possible to correlate a group of soluble EPS proteins with the SRT of 60 days, and a lower sludge age led to a lower diversity of polysaccharide sugar monomers, with an increase of glucose and galactose in the cake layer.
27270599	11	45	theme	MBR	1688:1690	arg1	design					1692:1697	MBR design	1688:1697	MBR design	1688:1697	This study improves our knowledge regarding the molecular reasons for fouling, which may contribute to improve MBR design and operation.
27270599	2	46	theme	crucial	366:372	arg1	parameter					374:382	a crucial parameter	364:382	a crucial parameter impacting microbial selection in the reactor	364:427	MBRs can be operated with long sludge retention time (SRT), a crucial parameter impacting microbial selection in the reactor.
27270599	2	46	theme	crucial	366:372	arg1	time					352:355	long sludge retention time	330:355	long sludge retention time (SRT)	330:361	MBRs can be operated with long sludge retention time (SRT), a crucial parameter impacting microbial selection in the reactor.
27270599	0	47	theme	time	27:30	arg1	Impact					0:5	Impact	0:5	Impact of sludge retention time on the fine composition of the microbial community and extracellular polymeric substances in a membrane bioreactor.	0:146	Impact of sludge retention time on the fine composition of the microbial community and extracellular polymeric substances in a membrane bioreactor.
27270599	6	48	theme	mixed	860:864	arg1	liquor					866:871	the mixed liquor	856:871	the mixed liquor	856:871	A comprehensive analysis of the microbial community structure and EPS proteins and polysaccharide profiles of the mixed liquor and cake layer was carried out throughout both operation periods.
27270599	7	49	theme	dominant	1031:1038	arg1	populations					1050:1060	the dominant bacterial populations	1027:1060	the dominant bacterial populations	1027:1060	The results of this study showed that the imposition of a shorter SRT led to a shift in the dominant bacterial populations.
27270599	7	50	theme	bacterial	1040:1048	arg1	populations					1050:1060	the dominant bacterial populations	1027:1060	the dominant bacterial populations	1027:1060	The results of this study showed that the imposition of a shorter SRT led to a shift in the dominant bacterial populations.
27270599	4	51	theme	SRT	583:585	arg1	impact					569:574	the impact	565:574	the impact of the SRT on the ecophysiology of the MBRs and, consequently, on membrane fouling	565:657	In this study, the impact of the SRT on the ecophysiology of the MBRs and, consequently, on membrane fouling was evaluated.
27270599	0	52	theme	fine	39:42	arg1	composition					44:54	the fine composition	35:54	the fine composition of the microbial community	35:81	Impact of sludge retention time on the fine composition of the microbial community and extracellular polymeric substances in a membrane bioreactor.
27270599	1	53	theme	membranes	293:301	arg1	fouling					278:284	rapid fouling	272:284	rapid fouling of the membranes	272:301	Membrane bioreactors (MBRs) are an advanced technology for wastewater treatment whose wide application has been hindered by rapid fouling of the membranes.
27270599	8	54	theme	layer	1089:1093	arg1	communities					1095:1105	The mixed liquor and cake layer communities	1063:1105	The mixed liquor and cake layer communities	1063:1105	The mixed liquor and cake layer communities were very different, with Actinomycetales order standing out in the cake layer at SRT of 20 days.
27270599	0	55	from	composition	44:54	arg1	bioreactor					136:145	a membrane bioreactor	125:145	a membrane bioreactor	125:145	Impact of sludge retention time on the fine composition of the microbial community and extracellular polymeric substances in a membrane bioreactor.
27270599	9	56	dep	concentrations	1225:1238	arg1	proteins					1254:1261	proteins	1254:1261	particularly proteins	1241:1261	Overall, higher EPS concentrations (particularly proteins) were found at this SRT.
27270599	8	57	theme	cake	1175:1178	arg1	layer					1180:1184	the cake layer	1171:1184	the cake layer	1171:1184	The mixed liquor and cake layer communities were very different, with Actinomycetales order standing out in the cake layer at SRT of 20 days.
27270599	5	58	theme	60 days	708:714	arg1	SRT					701:703	a SRT	699:703	a SRT of 60 days followed by a SRT of 20 days	699:743	A MBR was operated under a SRT of 60 days followed by a SRT of 20 days.
27270599	7	59	from	shift	1018:1022	arg1	populations					1050:1060	the dominant bacterial populations	1027:1060	the dominant bacterial populations	1027:1060	The results of this study showed that the imposition of a shorter SRT led to a shift in the dominant bacterial populations.
27270599	7	60	theme	shorter	997:1003	arg1	SRT					1005:1007	a shorter SRT	995:1007	a shorter SRT	995:1007	The results of this study showed that the imposition of a shorter SRT led to a shift in the dominant bacterial populations.
27270599	10	61	theme	EPS	1301:1303	arg1	profiles					1305:1312	EPS profiles	1301:1312	EPS profiles	1301:1312	Furthermore, EPS profiles were clearly affected by the SRT: it was possible to correlate a group of soluble EPS proteins with the SRT of 60 days, and a lower sludge age led to a lower diversity of polysaccharide sugar monomers, with an increase of glucose and galactose in the cake layer.
27270599	11	62	theme	molecular	1625:1633	arg1	reasons					1635:1641	the molecular reasons	1621:1641	the molecular reasons for fouling, which may contribute to improve MBR design and operation	1621:1711	This study improves our knowledge regarding the molecular reasons for fouling, which may contribute to improve MBR design and operation.
27270599	9	63	theme	EPS	1221:1223	arg1	concentrations					1225:1238	higher EPS concentrations	1214:1238	higher EPS concentrations (particularly proteins)	1214:1262	Overall, higher EPS concentrations (particularly proteins) were found at this SRT.
27270599	4	64	theme	MBRs	615:618	arg1	ecophysiology					594:606	the ecophysiology	590:606	the ecophysiology of the MBRs	590:618	In this study, the impact of the SRT on the ecophysiology of the MBRs and, consequently, on membrane fouling was evaluated.
27270599	1	65	theme	wastewater	207:216	arg1	treatment					218:226	wastewater treatment	207:226	wastewater treatment	207:226	Membrane bioreactors (MBRs) are an advanced technology for wastewater treatment whose wide application has been hindered by rapid fouling of the membranes.
27270599	10	66	theme	galactose	1548:1556	arg1	increase					1524:1531	an increase	1521:1531	an increase of glucose and galactose in the cake layer	1521:1574	Furthermore, EPS profiles were clearly affected by the SRT: it was possible to correlate a group of soluble EPS proteins with the SRT of 60 days, and a lower sludge age led to a lower diversity of polysaccharide sugar monomers, with an increase of glucose and galactose in the cake layer.
27270599	3	67	theme	filtration	448:457	arg1	performance					459:469	filtration performance	448:469	filtration performance	448:469	This also affects filtration performance, since a major fouling agent are the extracellular polymeric substances (EPS).
27270599	10	68	theme	EPS	1396:1398	arg1	proteins					1400:1407	soluble EPS proteins	1388:1407	soluble EPS proteins	1388:1407	Furthermore, EPS profiles were clearly affected by the SRT: it was possible to correlate a group of soluble EPS proteins with the SRT of 60 days, and a lower sludge age led to a lower diversity of polysaccharide sugar monomers, with an increase of glucose and galactose in the cake layer.
27270599	2	69	theme	microbial	394:402	arg1	selection					404:412	microbial selection	394:412	microbial selection	394:412	MBRs can be operated with long sludge retention time (SRT), a crucial parameter impacting microbial selection in the reactor.
27270599	3	70	theme	major	480:484	arg1	substances					532:541	the extracellular polymeric substances	504:541	the extracellular polymeric substances (EPS)	504:547	This also affects filtration performance, since a major fouling agent are the extracellular polymeric substances (EPS).
27270599	3	70	theme	major	480:484	arg1	agent					494:498	a major fouling agent	478:498	a major fouling agent	478:498	This also affects filtration performance, since a major fouling agent are the extracellular polymeric substances (EPS).
27270599	6	71	theme	operation	920:928	arg1	periods					930:936	both operation periods	915:936	both operation periods	915:936	A comprehensive analysis of the microbial community structure and EPS proteins and polysaccharide profiles of the mixed liquor and cake layer was carried out throughout both operation periods.
27270599	10	72	theme	soluble	1388:1394	arg1	proteins					1400:1407	soluble EPS proteins	1388:1407	soluble EPS proteins	1388:1407	Furthermore, EPS profiles were clearly affected by the SRT: it was possible to correlate a group of soluble EPS proteins with the SRT of 60 days, and a lower sludge age led to a lower diversity of polysaccharide sugar monomers, with an increase of glucose and galactose in the cake layer.
27270599	6	73	theme	polysaccharide	829:842	arg1	profiles					844:851	polysaccharide profiles	829:851	polysaccharide profiles	829:851	A comprehensive analysis of the microbial community structure and EPS proteins and polysaccharide profiles of the mixed liquor and cake layer was carried out throughout both operation periods.
27270599	0	74	from	substances	111:120	arg1	bioreactor					136:145	a membrane bioreactor	125:145	a membrane bioreactor	125:145	Impact of sludge retention time on the fine composition of the microbial community and extracellular polymeric substances in a membrane bioreactor.
27270599	2	75	theme	retention	342:350	arg1	parameter					374:382	a crucial parameter	364:382	a crucial parameter impacting microbial selection in the reactor	364:427	MBRs can be operated with long sludge retention time (SRT), a crucial parameter impacting microbial selection in the reactor.
27270599	2	75	theme	retention	342:350	arg1	SRT					358:360	SRT	358:360	SRT	358:360	MBRs can be operated with long sludge retention time (SRT), a crucial parameter impacting microbial selection in the reactor.
27270599	2	75	theme	retention	342:350	arg1	time					352:355	long sludge retention time	330:355	long sludge retention time (SRT)	330:361	MBRs can be operated with long sludge retention time (SRT), a crucial parameter impacting microbial selection in the reactor.
27270599	10	76	theme	sludge	1446:1451	arg1	age					1453:1455	a lower sludge age	1438:1455	a lower sludge age	1438:1455	Furthermore, EPS profiles were clearly affected by the SRT: it was possible to correlate a group of soluble EPS proteins with the SRT of 60 days, and a lower sludge age led to a lower diversity of polysaccharide sugar monomers, with an increase of glucose and galactose in the cake layer.
27270599	1	77	theme	wide	234:237	arg1	application					239:249	application	239:249	application	239:249	Membrane bioreactors (MBRs) are an advanced technology for wastewater treatment whose wide application has been hindered by rapid fouling of the membranes.
28867229	2	0	theme	network	373:379	arg1	structure					381:389	three dimensional network structure	355:389	three dimensional network structure	355:389	Microfibrillated cellulose with three dimensional network structure was prepared from microcrystalline cellulose by high-pressure homogenization.
28867229	1	1	dep	chitosan	258:265	arg1	fibrils					314:320	microfibrillated cellulose nano fibrils	282:320	microfibrillated cellulose nano fibrils	282:320	A novel composite film with efficient removal of anionic dye (Congo red) was developed using chitosan and dialdehyde microfibrillated cellulose nano fibrils.
28867229	8	2	theme	contact	1078:1084	arg1	time					1086:1089	contact time	1078:1089	contact time	1078:1089	The adsorption performance was investigated with respect to pH, temperature, contact time, and the initial dye concentration.
28867229	5	3	theme	DAMFC	660:664	arg1	compounding					645:655	the compounding	641:655	the compounding of DAMFC with chitosan	641:678	During the compounding of DAMFC with chitosan, a Schiff base was formed through the reaction between the aldehyde groups of DAMFC and amino groups of chitosan.
28867229	2	4	theme	dimensional	361:371	arg1	structure					381:389	three dimensional network structure	355:389	three dimensional network structure	355:389	Microfibrillated cellulose with three dimensional network structure was prepared from microcrystalline cellulose by high-pressure homogenization.
28867229	7	5	theme	chitosan	924:931	arg1	material					933:940	the chitosan material	920:940	the chitosan material	920:940	The addition of DAMFC resulted in remarkably increased adsorption capacity of the chitosan material as well as drastically improved stability in dye solution.
28867229	9	6	theme	possible	1131:1138	arg1	mechanism					1151:1159	The possible adsorption mechanism	1127:1159	The possible adsorption mechanism	1127:1159	The possible adsorption mechanism was proposed.
28867229	0	7	theme	composite	95:103	arg1	film					105:108	cellulose/chitosan composite film	76:108	cellulose/chitosan composite film	76:108	Efficient removal of anionic dye (Congo red) by dialdehyde microfibrillated cellulose/chitosan composite film with significantly improved stability in dye solution.
28867229	7	8	theme	material	933:940	arg1	capacity					908:915	remarkably increased adsorption capacity	876:915	remarkably increased adsorption capacity of the chitosan material as well as drastically improved stability in dye solution	876:998	The addition of DAMFC resulted in remarkably increased adsorption capacity of the chitosan material as well as drastically improved stability in dye solution.
28867229	7	8	theme	material	933:940	arg1	stability					974:982	drastically improved stability	953:982	remarkably increased adsorption capacity of the chitosan material as well as drastically improved stability in dye solution	876:998	The addition of DAMFC resulted in remarkably increased adsorption capacity of the chitosan material as well as drastically improved stability in dye solution.
28867229	0	9	theme	cellulose/chitosan	76:93	arg1	film					105:108	cellulose/chitosan composite film	76:108	cellulose/chitosan composite film	76:108	Efficient removal of anionic dye (Congo red) by dialdehyde microfibrillated cellulose/chitosan composite film with significantly improved stability in dye solution.
28867229	3	10	theme	dialdehyde	522:531	arg1	DAMFC					561:565	DAMFC	561:565	DAMFC	561:565	Then it was surface modified by periodate to prepare dialdehyde microfibrillated cellulose (DAMFC).
28867229	3	10	theme	dialdehyde	522:531	arg1	cellulose					550:558	dialdehyde microfibrillated cellulose	522:558	dialdehyde microfibrillated cellulose (DAMFC)	522:566	Then it was surface modified by periodate to prepare dialdehyde microfibrillated cellulose (DAMFC).
28867229	5	11	theme	chitosan	784:791	arg1	chitosan					784:791	chitosan	784:791	chitosan	784:791	During the compounding of DAMFC with chitosan, a Schiff base was formed through the reaction between the aldehyde groups of DAMFC and amino groups of chitosan.
28867229	5	11	theme	chitosan	784:791	arg1	DAMFC					758:762	DAMFC	758:762	DAMFC	758:762	During the compounding of DAMFC with chitosan, a Schiff base was formed through the reaction between the aldehyde groups of DAMFC and amino groups of chitosan.
28867229	5	11	theme	chitosan	784:791	arg1	groups					774:779	amino groups	768:779	amino groups of chitosan	768:791	During the compounding of DAMFC with chitosan, a Schiff base was formed through the reaction between the aldehyde groups of DAMFC and amino groups of chitosan.
28867229	5	11	theme	chitosan	784:791	arg1	groups					748:753	the aldehyde groups	735:753	the aldehyde groups of DAMFC	735:762	During the compounding of DAMFC with chitosan, a Schiff base was formed through the reaction between the aldehyde groups of DAMFC and amino groups of chitosan.
28867229	8	12	theme	initial	1100:1106	arg1	concentration					1112:1124	the initial dye concentration	1096:1124	the initial dye concentration	1096:1124	The adsorption performance was investigated with respect to pH, temperature, contact time, and the initial dye concentration.
28867229	1	13	theme	novel	167:171	arg1	film					183:186	A novel composite film	165:186	A novel composite film with efficient removal of anionic dye (Congo red)	165:236	A novel composite film with efficient removal of anionic dye (Congo red) was developed using chitosan and dialdehyde microfibrillated cellulose nano fibrils.
28867229	10	14	theme	kinetic	1235:1241	arg1	parameters					1243:1252	kinetic parameters	1235:1252	kinetic parameters	1235:1252	Various isotherm models have been used to fit the data, and kinetic parameters were evaluated.
28867229	5	15	theme	Schiff	683:688	arg1	base					690:693	a Schiff base	681:693	a Schiff base	681:693	During the compounding of DAMFC with chitosan, a Schiff base was formed through the reaction between the aldehyde groups of DAMFC and amino groups of chitosan.
28867229	7	16	theme	adsorption	897:906	arg1	capacity					908:915	remarkably increased adsorption capacity	876:915	remarkably increased adsorption capacity of the chitosan material as well as drastically improved stability in dye solution	876:998	The addition of DAMFC resulted in remarkably increased adsorption capacity of the chitosan material as well as drastically improved stability in dye solution.
28867229	1	17	theme	composite	173:181	arg1	film					183:186	A novel composite film	165:186	A novel composite film with efficient removal of anionic dye (Congo red)	165:236	A novel composite film with efficient removal of anionic dye (Congo red) was developed using chitosan and dialdehyde microfibrillated cellulose nano fibrils.
28867229	10	18	theme	Various	1175:1181	arg1	models					1192:1197	Various isotherm models	1175:1197	Various isotherm models	1175:1197	Various isotherm models have been used to fit the data, and kinetic parameters were evaluated.
28867229	9	19	theme	adsorption	1140:1149	arg1	mechanism					1151:1159	The possible adsorption mechanism	1127:1159	The possible adsorption mechanism	1127:1159	The possible adsorption mechanism was proposed.
28867229	0	20	theme	Efficient	0:8	arg1	removal					10:16	Efficient removal	0:16	Efficient removal of anionic dye (Congo red) by dialdehyde	0:57	Efficient removal of anionic dye (Congo red) by dialdehyde microfibrillated cellulose/chitosan composite film with significantly improved stability in dye solution.
28867229	5	21	with	compounding	645:655	arg1	chitosan					671:678	chitosan	671:678	chitosan	671:678	During the compounding of DAMFC with chitosan, a Schiff base was formed through the reaction between the aldehyde groups of DAMFC and amino groups of chitosan.
28867229	8	22	theme	dye	1108:1110	arg1	concentration					1112:1124	the initial dye concentration	1096:1124	the initial dye concentration	1096:1124	The adsorption performance was investigated with respect to pH, temperature, contact time, and the initial dye concentration.
28867229	0	23	theme	anionic	21:27	arg1	dye					29:31	anionic dye	21:31	anionic dye (Congo red)	21:43	Efficient removal of anionic dye (Congo red) by dialdehyde microfibrillated cellulose/chitosan composite film with significantly improved stability in dye solution.
28867229	7	24	theme	DAMFC	858:862	arg1	addition					846:853	The addition	842:853	The addition of DAMFC	842:862	The addition of DAMFC resulted in remarkably increased adsorption capacity of the chitosan material as well as drastically improved stability in dye solution.
28867229	0	25	theme	improved	129:136	arg1	stability					138:146	significantly improved stability	115:146	significantly improved stability in dye solution	115:162	Efficient removal of anionic dye (Congo red) by dialdehyde microfibrillated cellulose/chitosan composite film with significantly improved stability in dye solution.
28867229	7	26	theme	dye	987:989	arg1	solution					991:998	dye solution	987:998	dye solution	987:998	The addition of DAMFC resulted in remarkably increased adsorption capacity of the chitosan material as well as drastically improved stability in dye solution.
28867229	7	27	theme	increased	887:895	arg1	capacity					908:915	remarkably increased adsorption capacity	876:915	remarkably increased adsorption capacity of the chitosan material as well as drastically improved stability in dye solution	876:998	The addition of DAMFC resulted in remarkably increased adsorption capacity of the chitosan material as well as drastically improved stability in dye solution.
28867229	1	28	theme	efficient	193:201	arg1	removal					203:209	efficient removal	193:209	efficient removal of anionic dye (Congo red)	193:236	A novel composite film with efficient removal of anionic dye (Congo red) was developed using chitosan and dialdehyde microfibrillated cellulose nano fibrils.
28867229	10	29	theme	isotherm	1183:1190	arg1	models					1192:1197	Various isotherm models	1175:1197	Various isotherm models	1175:1197	Various isotherm models have been used to fit the data, and kinetic parameters were evaluated.
28867229	5	30	theme	DAMFC	758:762	arg1	chitosan					784:791	chitosan	784:791	chitosan	784:791	During the compounding of DAMFC with chitosan, a Schiff base was formed through the reaction between the aldehyde groups of DAMFC and amino groups of chitosan.
28867229	5	30	theme	DAMFC	758:762	arg1	DAMFC					758:762	DAMFC	758:762	DAMFC	758:762	During the compounding of DAMFC with chitosan, a Schiff base was formed through the reaction between the aldehyde groups of DAMFC and amino groups of chitosan.
28867229	5	30	theme	DAMFC	758:762	arg1	groups					774:779	amino groups	768:779	amino groups of chitosan	768:791	During the compounding of DAMFC with chitosan, a Schiff base was formed through the reaction between the aldehyde groups of DAMFC and amino groups of chitosan.
28867229	5	30	theme	DAMFC	758:762	arg1	groups					748:753	the aldehyde groups	735:753	the aldehyde groups of DAMFC	735:762	During the compounding of DAMFC with chitosan, a Schiff base was formed through the reaction between the aldehyde groups of DAMFC and amino groups of chitosan.
28867229	2	31	with	cellulose	340:348	arg1	structure					381:389	three dimensional network structure	355:389	three dimensional network structure	355:389	Microfibrillated cellulose with three dimensional network structure was prepared from microcrystalline cellulose by high-pressure homogenization.
28867229	7	32	from	stability	974:982	arg1	solution					991:998	dye solution	987:998	dye solution	987:998	The addition of DAMFC resulted in remarkably increased adsorption capacity of the chitosan material as well as drastically improved stability in dye solution.
28867229	1	33	theme	microfibrillated	282:297	arg1	fibrils					314:320	microfibrillated cellulose nano fibrils	282:320	microfibrillated cellulose nano fibrils	282:320	A novel composite film with efficient removal of anionic dye (Congo red) was developed using chitosan and dialdehyde microfibrillated cellulose nano fibrils.
28867229	10	34	used	used	1209:1212	arg2	models					1192:1197	Various isotherm models	1175:1197	Various isotherm models	1175:1197	Various isotherm models have been used to fit the data, and kinetic parameters were evaluated.
28867229	2	35	theme	high-pressure	439:451	arg1	homogenization					453:466	high-pressure homogenization	439:466	high-pressure homogenization	439:466	Microfibrillated cellulose with three dimensional network structure was prepared from microcrystalline cellulose by high-pressure homogenization.
28867229	0	36	theme	dye	29:31	arg1	removal					10:16	Efficient removal	0:16	Efficient removal of anionic dye (Congo red) by dialdehyde	0:57	Efficient removal of anionic dye (Congo red) by dialdehyde microfibrillated cellulose/chitosan composite film with significantly improved stability in dye solution.
28867229	1	37	theme	cellulose	299:307	arg1	fibrils					314:320	microfibrillated cellulose nano fibrils	282:320	microfibrillated cellulose nano fibrils	282:320	A novel composite film with efficient removal of anionic dye (Congo red) was developed using chitosan and dialdehyde microfibrillated cellulose nano fibrils.
28867229	5	38	theme	amino	768:772	arg1	chitosan					784:791	chitosan	784:791	chitosan	784:791	During the compounding of DAMFC with chitosan, a Schiff base was formed through the reaction between the aldehyde groups of DAMFC and amino groups of chitosan.
28867229	5	38	theme	amino	768:772	arg1	DAMFC					758:762	DAMFC	758:762	DAMFC	758:762	During the compounding of DAMFC with chitosan, a Schiff base was formed through the reaction between the aldehyde groups of DAMFC and amino groups of chitosan.
28867229	5	38	theme	amino	768:772	arg1	groups					774:779	amino groups	768:779	amino groups of chitosan	768:791	During the compounding of DAMFC with chitosan, a Schiff base was formed through the reaction between the aldehyde groups of DAMFC and amino groups of chitosan.
28867229	1	39	dep	dye	222:224	arg1	red					233:235	red	233:235	red	233:235	A novel composite film with efficient removal of anionic dye (Congo red) was developed using chitosan and dialdehyde microfibrillated cellulose nano fibrils.
28867229	1	40	theme	anionic	214:220	arg1	dye					222:224	anionic dye	214:224	anionic dye (Congo red)	214:236	A novel composite film with efficient removal of anionic dye (Congo red) was developed using chitosan and dialdehyde microfibrillated cellulose nano fibrils.
28867229	1	41	theme	nano	309:312	arg1	fibrils					314:320	microfibrillated cellulose nano fibrils	282:320	microfibrillated cellulose nano fibrils	282:320	A novel composite film with efficient removal of anionic dye (Congo red) was developed using chitosan and dialdehyde microfibrillated cellulose nano fibrils.
28867229	1	42	with	film	183:186	arg1	removal					203:209	efficient removal	193:209	efficient removal of anionic dye (Congo red)	193:236	A novel composite film with efficient removal of anionic dye (Congo red) was developed using chitosan and dialdehyde microfibrillated cellulose nano fibrils.
28867229	4	43	theme	composite	584:592	arg1	films					594:598	DAMFC/chitosan composite films	569:598	DAMFC/chitosan composite films	569:598	DAMFC/chitosan composite films were prepared by solvent-casting.
28867229	6	44	theme	network	802:808	arg1	structure					810:818	A giant network structure	794:818	A giant network structure	794:818	A giant network structure was therefore formed.
28867229	3	45	theme	microfibrillated	533:548	arg1	DAMFC					561:565	DAMFC	561:565	DAMFC	561:565	Then it was surface modified by periodate to prepare dialdehyde microfibrillated cellulose (DAMFC).
28867229	3	45	theme	microfibrillated	533:548	arg1	cellulose					550:558	dialdehyde microfibrillated cellulose	522:558	dialdehyde microfibrillated cellulose (DAMFC)	522:566	Then it was surface modified by periodate to prepare dialdehyde microfibrillated cellulose (DAMFC).
28867229	0	46	theme	dye	151:153	arg1	solution					155:162	dye solution	151:162	dye solution	151:162	Efficient removal of anionic dye (Congo red) by dialdehyde microfibrillated cellulose/chitosan composite film with significantly improved stability in dye solution.
28867229	0	47	dep	dye	29:31	arg1	red					40:42	red	40:42	red	40:42	Efficient removal of anionic dye (Congo red) by dialdehyde microfibrillated cellulose/chitosan composite film with significantly improved stability in dye solution.
28867229	6	48	theme	giant	796:800	arg1	structure					810:818	A giant network structure	794:818	A giant network structure	794:818	A giant network structure was therefore formed.
28867229	3	49	mod	modified	489:496	arg1	it					474:475	it	474:475	it	474:475	Then it was surface modified by periodate to prepare dialdehyde microfibrillated cellulose (DAMFC).
28867229	3	49	mod	modified	489:496	arg3	periodate					501:509	periodate	501:509	periodate	501:509	Then it was surface modified by periodate to prepare dialdehyde microfibrillated cellulose (DAMFC).
28867229	7	50	theme	improved	965:972	arg1	stability					974:982	drastically improved stability	953:982	remarkably increased adsorption capacity of the chitosan material as well as drastically improved stability in dye solution	876:998	The addition of DAMFC resulted in remarkably increased adsorption capacity of the chitosan material as well as drastically improved stability in dye solution.
28867229	5	51	theme	aldehyde	739:746	arg1	chitosan					784:791	chitosan	784:791	chitosan	784:791	During the compounding of DAMFC with chitosan, a Schiff base was formed through the reaction between the aldehyde groups of DAMFC and amino groups of chitosan.
28867229	5	51	theme	aldehyde	739:746	arg1	DAMFC					758:762	DAMFC	758:762	DAMFC	758:762	During the compounding of DAMFC with chitosan, a Schiff base was formed through the reaction between the aldehyde groups of DAMFC and amino groups of chitosan.
28867229	5	51	theme	aldehyde	739:746	arg1	groups					748:753	the aldehyde groups	735:753	the aldehyde groups of DAMFC	735:762	During the compounding of DAMFC with chitosan, a Schiff base was formed through the reaction between the aldehyde groups of DAMFC and amino groups of chitosan.
28867229	3	52	dep	modified	489:496	arg1	surface					481:487	surface	481:487	surface	481:487	Then it was surface modified by periodate to prepare dialdehyde microfibrillated cellulose (DAMFC).
28867229	1	53	theme	dye	222:224	arg1	removal					203:209	efficient removal	193:209	efficient removal of anionic dye (Congo red)	193:236	A novel composite film with efficient removal of anionic dye (Congo red) was developed using chitosan and dialdehyde microfibrillated cellulose nano fibrils.
28867229	7	54	from	capacity	908:915	arg1	solution					991:998	dye solution	987:998	dye solution	987:998	The addition of DAMFC resulted in remarkably increased adsorption capacity of the chitosan material as well as drastically improved stability in dye solution.
28867229	8	55	theme	adsorption	1005:1014	arg1	performance					1016:1026	The adsorption performance	1001:1026	The adsorption performance	1001:1026	The adsorption performance was investigated with respect to pH, temperature, contact time, and the initial dye concentration.
28867229	2	56	theme	Microfibrillated	323:338	arg1	cellulose					340:348	Microfibrillated cellulose	323:348	Microfibrillated cellulose with three dimensional network structure	323:389	Microfibrillated cellulose with three dimensional network structure was prepared from microcrystalline cellulose by high-pressure homogenization.
28867229	0	57	from	stability	138:146	arg1	solution					155:162	dye solution	151:162	dye solution	151:162	Efficient removal of anionic dye (Congo red) by dialdehyde microfibrillated cellulose/chitosan composite film with significantly improved stability in dye solution.
28867229	2	58	theme	microcrystalline	409:424	arg1	cellulose					426:434	microcrystalline cellulose	409:434	microcrystalline cellulose	409:434	Microfibrillated cellulose with three dimensional network structure was prepared from microcrystalline cellulose by high-pressure homogenization.
28867229	4	59	theme	DAMFC/chitosan	569:582	arg1	films					594:598	DAMFC/chitosan composite films	569:598	DAMFC/chitosan composite films	569:598	DAMFC/chitosan composite films were prepared by solvent-casting.
26501272	7	0	theme	X-ray	1125:1129	arg1	XRD					1144:1146	XRD	1144:1146	XRD	1144:1146	Turbidity measurements and X-ray diffraction (XRD) data also indicated that the optimum complexation ratio for IPC between SDCH-NF/HA is 1/1, resulting in a good relationship between turbidity or XRD of the complex and the release ratio of FMT.
26501272	7	0	theme	X-ray	1125:1129	arg1	diffraction					1131:1141	X-ray diffraction	1125:1141	X-ray diffraction (XRD) data	1125:1152	Turbidity measurements and X-ray diffraction (XRD) data also indicated that the optimum complexation ratio for IPC between SDCH-NF/HA is 1/1, resulting in a good relationship between turbidity or XRD of the complex and the release ratio of FMT.
26501272	8	1	with	tablet	1444:1449	arg1	activity					1475:1482	a high antioxidant activity	1456:1482	a high antioxidant activity	1456:1482	These results suggest that an SDCH-NF/HA tablet has the potential for use in an extended-release IPC tablet with a high antioxidant activity.
26501272	7	2	theme	complexation	1186:1197	arg1	ratio					1199:1203	the optimum complexation ratio	1174:1203	the optimum complexation ratio for IPC between SDCH-NF/HA	1174:1230	Turbidity measurements and X-ray diffraction (XRD) data also indicated that the optimum complexation ratio for IPC between SDCH-NF/HA is 1/1, resulting in a good relationship between turbidity or XRD of the complex and the release ratio of FMT.
26501272	7	2	theme	complexation	1186:1197	arg1	1/1					1235:1237	1/1	1235:1237	1/1	1235:1237	Turbidity measurements and X-ray diffraction (XRD) data also indicated that the optimum complexation ratio for IPC between SDCH-NF/HA is 1/1, resulting in a good relationship between turbidity or XRD of the complex and the release ratio of FMT.
26501272	8	3	contain	has	1391:1393	arg1	tablet					1384:1389	an SDCH-NF/HA tablet	1370:1389	an SDCH-NF/HA tablet	1370:1389	These results suggest that an SDCH-NF/HA tablet has the potential for use in an extended-release IPC tablet with a high antioxidant activity.
26501272	8	3	contain	has	1391:1393	arg2	potential					1399:1407	the potential	1395:1407	the potential for use	1395:1415	These results suggest that an SDCH-NF/HA tablet has the potential for use in an extended-release IPC tablet with a high antioxidant activity.
26501272	2	4	theme	N-centered	466:475	arg1	radicals					477:484	N-centered radicals	466:484	N-centered radicals derived from 1,1'-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS)	466:601	The antioxidant properties of untreated chitin (UCH), SDCH-NF, and HA were examined using N-centered radicals derived from 1,1'-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS).
26501272	8	5	theme	IPC	1440:1442	arg1	tablet					1444:1449	an extended-release IPC tablet	1420:1449	an extended-release IPC tablet with a high antioxidant activity	1420:1482	These results suggest that an SDCH-NF/HA tablet has the potential for use in an extended-release IPC tablet with a high antioxidant activity.
26501272	1	6	theme	possible	176:183	arg1	use					185:187	a possible use	174:187	a possible use of a surface-deacetylated chitin nano-fiber (SDCH-NF) and hyaluronic acid (HA) interpolymer complex (IPC) tablet as a potential antioxidative compound in extended-release matrix tablets	174:373	In this study, we examined a possible use of a surface-deacetylated chitin nano-fiber (SDCH-NF) and hyaluronic acid (HA) interpolymer complex (IPC) tablet as a potential antioxidative compound in extended-release matrix tablets.
26501272	8	7	theme	extended-release	1423:1438	arg1	tablet					1444:1449	an extended-release IPC tablet	1420:1449	an extended-release IPC tablet with a high antioxidant activity	1420:1482	These results suggest that an SDCH-NF/HA tablet has the potential for use in an extended-release IPC tablet with a high antioxidant activity.
26501272	0	8	theme	Matrix	131:136	arg1	Tablets					138:144	Extended-Release Matrix Tablets	114:144	Extended-Release Matrix Tablets	114:144	Surface-Deacetylated Chitin Nano-Fiber/Hyaluronic Acid Composites as Potential Antioxidative Compounds for Use in Extended-Release Matrix Tablets.
26501272	1	9	theme	extended-release	343:358	arg1	tablets					367:373	extended-release matrix tablets	343:373	extended-release matrix tablets	343:373	In this study, we examined a possible use of a surface-deacetylated chitin nano-fiber (SDCH-NF) and hyaluronic acid (HA) interpolymer complex (IPC) tablet as a potential antioxidative compound in extended-release matrix tablets.
26501272	0	10	theme	Extended-Release	114:129	arg1	Tablets					138:144	Extended-Release Matrix Tablets	114:144	Extended-Release Matrix Tablets	114:144	Surface-Deacetylated Chitin Nano-Fiber/Hyaluronic Acid Composites as Potential Antioxidative Compounds for Use in Extended-Release Matrix Tablets.
26501272	1	11	theme	matrix	360:365	arg1	tablets					367:373	extended-release matrix tablets	343:373	extended-release matrix tablets	343:373	In this study, we examined a possible use of a surface-deacetylated chitin nano-fiber (SDCH-NF) and hyaluronic acid (HA) interpolymer complex (IPC) tablet as a potential antioxidative compound in extended-release matrix tablets.
26501272	1	12	theme	interpolymer	268:279	arg1	tablet					295:300	interpolymer complex (IPC) tablet	268:300	interpolymer complex (IPC) tablet	268:300	In this study, we examined a possible use of a surface-deacetylated chitin nano-fiber (SDCH-NF) and hyaluronic acid (HA) interpolymer complex (IPC) tablet as a potential antioxidative compound in extended-release matrix tablets.
26501272	4	13	theme	oxidative	847:855	arg1	stress					857:862	oxidative stress	847:862	oxidative stress	847:862	The results suggest that SDCH-NF and HA could serve as scavengers of compounds related to the development of oxidative stress.
26501272	7	14	theme	good	1255:1258	arg1	relationship					1260:1271	a good relationship	1253:1271	a good relationship between turbidity or XRD of the complex and the release ratio of FMT	1253:1340	Turbidity measurements and X-ray diffraction (XRD) data also indicated that the optimum complexation ratio for IPC between SDCH-NF/HA is 1/1, resulting in a good relationship between turbidity or XRD of the complex and the release ratio of FMT.
26501272	2	15	theme	untreated	406:414	arg1	UCH					424:426	UCH	424:426	UCH	424:426	The antioxidant properties of untreated chitin (UCH), SDCH-NF, and HA were examined using N-centered radicals derived from 1,1'-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS).
26501272	2	15	theme	untreated	406:414	arg1	chitin					416:421	untreated chitin	406:421	untreated chitin (UCH)	406:427	The antioxidant properties of untreated chitin (UCH), SDCH-NF, and HA were examined using N-centered radicals derived from 1,1'-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS).
26501272	1	16	from	compound	331:338	arg1	tablets					367:373	extended-release matrix tablets	343:373	extended-release matrix tablets	343:373	In this study, we examined a possible use of a surface-deacetylated chitin nano-fiber (SDCH-NF) and hyaluronic acid (HA) interpolymer complex (IPC) tablet as a potential antioxidative compound in extended-release matrix tablets.
26501272	8	17	theme	high	1458:1461	arg1	activity					1475:1482	a high antioxidant activity	1456:1482	a high antioxidant activity	1456:1482	These results suggest that an SDCH-NF/HA tablet has the potential for use in an extended-release IPC tablet with a high antioxidant activity.
26501272	5	18	theme	SDCH-NF/HA	868:877	arg1	matrix					947:952	an extended-release tablet matrix	920:952	an extended-release tablet matrix using famotidine (FMT) as a model drug	920:991	An SDCH-NF/HA IPC tablet was prepared and evaluated as an extended-release tablet matrix using famotidine (FMT) as a model drug.
26501272	5	18	theme	SDCH-NF/HA	868:877	arg1	tablet					883:888	An SDCH-NF/HA IPC tablet	865:888	An SDCH-NF/HA IPC tablet	865:888	An SDCH-NF/HA IPC tablet was prepared and evaluated as an extended-release tablet matrix using famotidine (FMT) as a model drug.
26501272	0	19	theme	Chitin	21:26	arg1	Composites					55:64	Surface-Deacetylated Chitin Nano-Fiber/Hyaluronic Acid Composites	0:64	Surface-Deacetylated Chitin Nano-Fiber/Hyaluronic Acid Composites as Potential Antioxidative Compounds for Use in Extended-Release Matrix Tablets.	0:145	Surface-Deacetylated Chitin Nano-Fiber/Hyaluronic Acid Composites as Potential Antioxidative Compounds for Use in Extended-Release Matrix Tablets.
26501272	7	20	theme	FMT	1338:1340	arg1	ratio					1329:1333	the release ratio	1317:1333	the release ratio of FMT	1317:1340	Turbidity measurements and X-ray diffraction (XRD) data also indicated that the optimum complexation ratio for IPC between SDCH-NF/HA is 1/1, resulting in a good relationship between turbidity or XRD of the complex and the release ratio of FMT.
26501272	7	20	theme	FMT	1338:1340	arg1	XRD					1294:1296	XRD	1294:1296	XRD	1294:1296	Turbidity measurements and X-ray diffraction (XRD) data also indicated that the optimum complexation ratio for IPC between SDCH-NF/HA is 1/1, resulting in a good relationship between turbidity or XRD of the complex and the release ratio of FMT.
26501272	7	20	theme	FMT	1338:1340	arg1	turbidity					1281:1289	turbidity	1281:1289	turbidity	1281:1289	Turbidity measurements and X-ray diffraction (XRD) data also indicated that the optimum complexation ratio for IPC between SDCH-NF/HA is 1/1, resulting in a good relationship between turbidity or XRD of the complex and the release ratio of FMT.
26501272	5	21	theme	IPC	879:881	arg1	matrix					947:952	an extended-release tablet matrix	920:952	an extended-release tablet matrix using famotidine (FMT) as a model drug	920:991	An SDCH-NF/HA IPC tablet was prepared and evaluated as an extended-release tablet matrix using famotidine (FMT) as a model drug.
26501272	5	21	theme	IPC	879:881	arg1	tablet					883:888	An SDCH-NF/HA IPC tablet	865:888	An SDCH-NF/HA IPC tablet	865:888	An SDCH-NF/HA IPC tablet was prepared and evaluated as an extended-release tablet matrix using famotidine (FMT) as a model drug.
26501272	0	22	theme	Surface-Deacetylated	0:19	arg1	Composites					55:64	Surface-Deacetylated Chitin Nano-Fiber/Hyaluronic Acid Composites	0:64	Surface-Deacetylated Chitin Nano-Fiber/Hyaluronic Acid Composites as Potential Antioxidative Compounds for Use in Extended-Release Matrix Tablets.	0:145	Surface-Deacetylated Chitin Nano-Fiber/Hyaluronic Acid Composites as Potential Antioxidative Compounds for Use in Extended-Release Matrix Tablets.
26501272	1	23	theme	complex	281:287	arg1	tablet					295:300	interpolymer complex (IPC) tablet	268:300	interpolymer complex (IPC) tablet	268:300	In this study, we examined a possible use of a surface-deacetylated chitin nano-fiber (SDCH-NF) and hyaluronic acid (HA) interpolymer complex (IPC) tablet as a potential antioxidative compound in extended-release matrix tablets.
26501272	4	24	theme	stress	857:862	arg1	development					832:842	the development	828:842	the development of oxidative stress	828:862	The results suggest that SDCH-NF and HA could serve as scavengers of compounds related to the development of oxidative stress.
26501272	6	25	dep	DCF-NF	1034:1039	arg1	HA=1:1					1041:1046	HA=1:1	1041:1046	DCF-NF:HA=1:1	1034:1046	The release of FMT from the IPC tablet (DCF-NF:HA=1:1) was slower than that from a SDCH-NF only tablet.
26501272	1	26	theme	surface-deacetylated	194:213	arg1	nano-fiber					222:231	a surface-deacetylated chitin nano-fiber	192:231	a surface-deacetylated chitin nano-fiber (SDCH-NF)	192:241	In this study, we examined a possible use of a surface-deacetylated chitin nano-fiber (SDCH-NF) and hyaluronic acid (HA) interpolymer complex (IPC) tablet as a potential antioxidative compound in extended-release matrix tablets.
26501272	1	26	theme	surface-deacetylated	194:213	arg1	SDCH-NF					234:240	SDCH-NF	234:240	SDCH-NF	234:240	In this study, we examined a possible use of a surface-deacetylated chitin nano-fiber (SDCH-NF) and hyaluronic acid (HA) interpolymer complex (IPC) tablet as a potential antioxidative compound in extended-release matrix tablets.
26501272	0	27	theme	Acid	50:53	arg1	Composites					55:64	Surface-Deacetylated Chitin Nano-Fiber/Hyaluronic Acid Composites	0:64	Surface-Deacetylated Chitin Nano-Fiber/Hyaluronic Acid Composites as Potential Antioxidative Compounds for Use in Extended-Release Matrix Tablets.	0:145	Surface-Deacetylated Chitin Nano-Fiber/Hyaluronic Acid Composites as Potential Antioxidative Compounds for Use in Extended-Release Matrix Tablets.
26501272	8	28	theme	antioxidant	1463:1473	arg1	activity					1475:1482	a high antioxidant activity	1456:1482	a high antioxidant activity	1456:1482	These results suggest that an SDCH-NF/HA tablet has the potential for use in an extended-release IPC tablet with a high antioxidant activity.
26501272	2	29	theme	3-ethylbenzothiazoline-6-sulfonic	556:588	arg1	2,2'-azinobis					541:553	2,2'-azinobis	541:553	2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS)	541:601	The antioxidant properties of untreated chitin (UCH), SDCH-NF, and HA were examined using N-centered radicals derived from 1,1'-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS).
26501272	2	29	theme	3-ethylbenzothiazoline-6-sulfonic	556:588	arg1	acid					590:593	3-ethylbenzothiazoline-6-sulfonic acid	556:593	3-ethylbenzothiazoline-6-sulfonic acid	556:593	The antioxidant properties of untreated chitin (UCH), SDCH-NF, and HA were examined using N-centered radicals derived from 1,1'-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS).
26501272	6	30	from	tablet	1026:1031	arg1	slower					1053:1058	slower	1053:1058	slower	1053:1058	The release of FMT from the IPC tablet (DCF-NF:HA=1:1) was slower than that from a SDCH-NF only tablet.
26501272	6	30	from	tablet	1026:1031	arg1	release					998:1004	The release	994:1004	The release of FMT from the IPC tablet (DCF-NF:HA=1:1)	994:1047	The release of FMT from the IPC tablet (DCF-NF:HA=1:1) was slower than that from a SDCH-NF only tablet.
26501272	1	31	theme	chitin	215:220	arg1	nano-fiber					222:231	a surface-deacetylated chitin nano-fiber	192:231	a surface-deacetylated chitin nano-fiber (SDCH-NF)	192:241	In this study, we examined a possible use of a surface-deacetylated chitin nano-fiber (SDCH-NF) and hyaluronic acid (HA) interpolymer complex (IPC) tablet as a potential antioxidative compound in extended-release matrix tablets.
26501272	1	31	theme	chitin	215:220	arg1	SDCH-NF					234:240	SDCH-NF	234:240	SDCH-NF	234:240	In this study, we examined a possible use of a surface-deacetylated chitin nano-fiber (SDCH-NF) and hyaluronic acid (HA) interpolymer complex (IPC) tablet as a potential antioxidative compound in extended-release matrix tablets.
26501272	0	32	theme	Nano-Fiber/Hyaluronic	28:48	arg1	Composites					55:64	Surface-Deacetylated Chitin Nano-Fiber/Hyaluronic Acid Composites	0:64	Surface-Deacetylated Chitin Nano-Fiber/Hyaluronic Acid Composites as Potential Antioxidative Compounds for Use in Extended-Release Matrix Tablets.	0:145	Surface-Deacetylated Chitin Nano-Fiber/Hyaluronic Acid Composites as Potential Antioxidative Compounds for Use in Extended-Release Matrix Tablets.
26501272	1	33	theme	IPC	290:292	arg1	tablet					295:300	interpolymer complex (IPC) tablet	268:300	interpolymer complex (IPC) tablet	268:300	In this study, we examined a possible use of a surface-deacetylated chitin nano-fiber (SDCH-NF) and hyaluronic acid (HA) interpolymer complex (IPC) tablet as a potential antioxidative compound in extended-release matrix tablets.
26501272	8	34	theme	SDCH-NF/HA	1373:1382	arg1	tablet					1384:1389	an SDCH-NF/HA tablet	1370:1389	an SDCH-NF/HA tablet	1370:1389	These results suggest that an SDCH-NF/HA tablet has the potential for use in an extended-release IPC tablet with a high antioxidant activity.
26501272	1	35	theme	nano-fiber	222:231	arg1	use					185:187	a possible use	174:187	a possible use of a surface-deacetylated chitin nano-fiber (SDCH-NF) and hyaluronic acid (HA) interpolymer complex (IPC) tablet as a potential antioxidative compound in extended-release matrix tablets	174:373	In this study, we examined a possible use of a surface-deacetylated chitin nano-fiber (SDCH-NF) and hyaluronic acid (HA) interpolymer complex (IPC) tablet as a potential antioxidative compound in extended-release matrix tablets.
26501272	7	36	theme	Turbidity	1098:1106	arg1	measurements					1108:1119	Turbidity measurements	1098:1119	Turbidity measurements	1098:1119	Turbidity measurements and X-ray diffraction (XRD) data also indicated that the optimum complexation ratio for IPC between SDCH-NF/HA is 1/1, resulting in a good relationship between turbidity or XRD of the complex and the release ratio of FMT.
26501272	0	37	from	Use	107:109	arg1	Tablets					138:144	Extended-Release Matrix Tablets	114:144	Extended-Release Matrix Tablets	114:144	Surface-Deacetylated Chitin Nano-Fiber/Hyaluronic Acid Composites as Potential Antioxidative Compounds for Use in Extended-Release Matrix Tablets.
26501272	2	38	theme	antioxidant	380:390	arg1	properties					392:401	The antioxidant properties	376:401	The antioxidant properties of untreated chitin (UCH), SDCH-NF, and HA	376:444	The antioxidant properties of untreated chitin (UCH), SDCH-NF, and HA were examined using N-centered radicals derived from 1,1'-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS).
26501272	6	39	theme	IPC	1022:1024	arg1	tablet					1026:1031	the IPC tablet	1018:1031	the IPC tablet (DCF-NF:HA=1:1)	1018:1047	The release of FMT from the IPC tablet (DCF-NF:HA=1:1) was slower than that from a SDCH-NF only tablet.
26501272	6	39	theme	IPC	1022:1024	arg1	DCF-NF					1034:1039	DCF-NF	1034:1039	DCF-NF:HA=1:1	1034:1046	The release of FMT from the IPC tablet (DCF-NF:HA=1:1) was slower than that from a SDCH-NF only tablet.
26501272	2	40	theme	SDCH-NF	430:436	arg1	properties					392:401	The antioxidant properties	376:401	The antioxidant properties of untreated chitin (UCH), SDCH-NF, and HA	376:444	The antioxidant properties of untreated chitin (UCH), SDCH-NF, and HA were examined using N-centered radicals derived from 1,1'-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS).
26501272	2	41	theme	chitin	416:421	arg1	properties					392:401	The antioxidant properties	376:401	The antioxidant properties of untreated chitin (UCH), SDCH-NF, and HA	376:444	The antioxidant properties of untreated chitin (UCH), SDCH-NF, and HA were examined using N-centered radicals derived from 1,1'-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS).
26501272	0	42	theme	Antioxidative	79:91	arg1	Compounds					93:101	Potential Antioxidative Compounds	69:101	Potential Antioxidative Compounds for Use in Extended-Release Matrix Tablets	69:144	Surface-Deacetylated Chitin Nano-Fiber/Hyaluronic Acid Composites as Potential Antioxidative Compounds for Use in Extended-Release Matrix Tablets.
26501272	7	43	theme	release	1321:1327	arg1	ratio					1329:1333	the release ratio	1317:1333	the release ratio of FMT	1317:1340	Turbidity measurements and X-ray diffraction (XRD) data also indicated that the optimum complexation ratio for IPC between SDCH-NF/HA is 1/1, resulting in a good relationship between turbidity or XRD of the complex and the release ratio of FMT.
26501272	7	44	theme	complex	1305:1311	arg1	ratio					1329:1333	the release ratio	1317:1333	the release ratio of FMT	1317:1340	Turbidity measurements and X-ray diffraction (XRD) data also indicated that the optimum complexation ratio for IPC between SDCH-NF/HA is 1/1, resulting in a good relationship between turbidity or XRD of the complex and the release ratio of FMT.
26501272	7	44	theme	complex	1305:1311	arg1	XRD					1294:1296	XRD	1294:1296	XRD	1294:1296	Turbidity measurements and X-ray diffraction (XRD) data also indicated that the optimum complexation ratio for IPC between SDCH-NF/HA is 1/1, resulting in a good relationship between turbidity or XRD of the complex and the release ratio of FMT.
26501272	7	44	theme	complex	1305:1311	arg1	turbidity					1281:1289	turbidity	1281:1289	turbidity	1281:1289	Turbidity measurements and X-ray diffraction (XRD) data also indicated that the optimum complexation ratio for IPC between SDCH-NF/HA is 1/1, resulting in a good relationship between turbidity or XRD of the complex and the release ratio of FMT.
26501272	6	45	theme	only	1085:1088	arg1	tablet					1090:1095	a SDCH-NF only tablet	1075:1095	a SDCH-NF only tablet	1075:1095	The release of FMT from the IPC tablet (DCF-NF:HA=1:1) was slower than that from a SDCH-NF only tablet.
26501272	0	46	theme	Potential	69:77	arg1	Compounds					93:101	Potential Antioxidative Compounds	69:101	Potential Antioxidative Compounds for Use in Extended-Release Matrix Tablets	69:144	Surface-Deacetylated Chitin Nano-Fiber/Hyaluronic Acid Composites as Potential Antioxidative Compounds for Use in Extended-Release Matrix Tablets.
26501272	2	47	theme	HA	443:444	arg1	properties					392:401	The antioxidant properties	376:401	The antioxidant properties of untreated chitin (UCH), SDCH-NF, and HA	376:444	The antioxidant properties of untreated chitin (UCH), SDCH-NF, and HA were examined using N-centered radicals derived from 1,1'-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS).
26501272	6	48	theme	SDCH-NF	1077:1083	arg1	tablet					1090:1095	a SDCH-NF only tablet	1075:1095	a SDCH-NF only tablet	1075:1095	The release of FMT from the IPC tablet (DCF-NF:HA=1:1) was slower than that from a SDCH-NF only tablet.
26501272	1	49	theme	potential	307:315	arg1	compound					331:338	a potential antioxidative compound	305:338	a potential antioxidative compound in extended-release matrix tablets	305:373	In this study, we examined a possible use of a surface-deacetylated chitin nano-fiber (SDCH-NF) and hyaluronic acid (HA) interpolymer complex (IPC) tablet as a potential antioxidative compound in extended-release matrix tablets.
26501272	5	50	theme	model	982:986	arg1	famotidine					960:969	famotidine	960:969	famotidine (FMT)	960:975	An SDCH-NF/HA IPC tablet was prepared and evaluated as an extended-release tablet matrix using famotidine (FMT) as a model drug.
26501272	5	50	theme	model	982:986	arg1	drug					988:991	a model drug	980:991	a model drug	980:991	An SDCH-NF/HA IPC tablet was prepared and evaluated as an extended-release tablet matrix using famotidine (FMT) as a model drug.
26501272	3	51	theme	acceptable	623:632	arg1	abilities					645:653	acceptable scavenging abilities	623:653	acceptable scavenging abilities	623:653	SDCH-NF and HA had acceptable scavenging abilities and were relatively efficient radical scavengers, but UCH was much less effective.
26501272	4	52	theme	related	817:823	arg1	compounds					807:815	compounds	807:815	compounds related to the development of oxidative stress	807:862	The results suggest that SDCH-NF and HA could serve as scavengers of compounds related to the development of oxidative stress.
26501272	5	53	theme	extended-release	923:938	arg1	matrix					947:952	an extended-release tablet matrix	920:952	an extended-release tablet matrix using famotidine (FMT) as a model drug	920:991	An SDCH-NF/HA IPC tablet was prepared and evaluated as an extended-release tablet matrix using famotidine (FMT) as a model drug.
26501272	5	53	theme	extended-release	923:938	arg1	tablet					883:888	An SDCH-NF/HA IPC tablet	865:888	An SDCH-NF/HA IPC tablet	865:888	An SDCH-NF/HA IPC tablet was prepared and evaluated as an extended-release tablet matrix using famotidine (FMT) as a model drug.
26501272	3	54	contain	had	619:621	arg1	SDCH-NF					604:610	SDCH-NF	604:610	SDCH-NF	604:610	SDCH-NF and HA had acceptable scavenging abilities and were relatively efficient radical scavengers, but UCH was much less effective.
26501272	3	54	contain	had	619:621	arg2	abilities					645:653	acceptable scavenging abilities	623:653	acceptable scavenging abilities	623:653	SDCH-NF and HA had acceptable scavenging abilities and were relatively efficient radical scavengers, but UCH was much less effective.
26501272	3	54	contain	had	619:621	arg1	HA					616:617	HA	616:617	HA	616:617	SDCH-NF and HA had acceptable scavenging abilities and were relatively efficient radical scavengers, but UCH was much less effective.
26501272	3	55	theme	efficient	675:683	arg1	scavengers					693:702	relatively efficient radical scavengers	664:702	relatively efficient radical scavengers	664:702	SDCH-NF and HA had acceptable scavenging abilities and were relatively efficient radical scavengers, but UCH was much less effective.
26501272	3	56	theme	scavenging	634:643	arg1	abilities					645:653	acceptable scavenging abilities	623:653	acceptable scavenging abilities	623:653	SDCH-NF and HA had acceptable scavenging abilities and were relatively efficient radical scavengers, but UCH was much less effective.
26501272	2	57	attach	derived	486:492	arg1	DPPH					531:534	DPPH	531:534	DPPH	531:534	The antioxidant properties of untreated chitin (UCH), SDCH-NF, and HA were examined using N-centered radicals derived from 1,1'-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS).
26501272	2	57	attach	derived	486:492	arg1	acid					590:593	3-ethylbenzothiazoline-6-sulfonic acid	556:593	3-ethylbenzothiazoline-6-sulfonic acid	556:593	The antioxidant properties of untreated chitin (UCH), SDCH-NF, and HA were examined using N-centered radicals derived from 1,1'-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS).
26501272	2	57	attach	derived	486:492	arg1	ABTS					597:600	ABTS	597:600	ABTS	597:600	The antioxidant properties of untreated chitin (UCH), SDCH-NF, and HA were examined using N-centered radicals derived from 1,1'-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS).
26501272	2	57	attach	derived	486:492	arg2	radicals					477:484	N-centered radicals	466:484	N-centered radicals derived from 1,1'-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS)	466:601	The antioxidant properties of untreated chitin (UCH), SDCH-NF, and HA were examined using N-centered radicals derived from 1,1'-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS).
26501272	2	57	attach	derived	486:492	arg1	1,1'-diphenyl-2-picrylhydrazyl					499:528	1,1'-diphenyl-2-picrylhydrazyl	499:528	1,1'-diphenyl-2-picrylhydrazyl (DPPH)	499:535	The antioxidant properties of untreated chitin (UCH), SDCH-NF, and HA were examined using N-centered radicals derived from 1,1'-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS).
26501272	2	57	attach	derived	486:492	arg1	2,2'-azinobis					541:553	2,2'-azinobis	541:553	2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS)	541:601	The antioxidant properties of untreated chitin (UCH), SDCH-NF, and HA were examined using N-centered radicals derived from 1,1'-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS).
26501272	5	58	theme	tablet	940:945	arg1	matrix					947:952	an extended-release tablet matrix	920:952	an extended-release tablet matrix using famotidine (FMT) as a model drug	920:991	An SDCH-NF/HA IPC tablet was prepared and evaluated as an extended-release tablet matrix using famotidine (FMT) as a model drug.
26501272	5	58	theme	tablet	940:945	arg1	tablet					883:888	An SDCH-NF/HA IPC tablet	865:888	An SDCH-NF/HA IPC tablet	865:888	An SDCH-NF/HA IPC tablet was prepared and evaluated as an extended-release tablet matrix using famotidine (FMT) as a model drug.
26501272	6	59	theme	FMT	1009:1011	arg1	slower					1053:1058	slower	1053:1058	slower	1053:1058	The release of FMT from the IPC tablet (DCF-NF:HA=1:1) was slower than that from a SDCH-NF only tablet.
26501272	6	59	theme	FMT	1009:1011	arg1	release					998:1004	The release	994:1004	The release of FMT from the IPC tablet (DCF-NF:HA=1:1)	994:1047	The release of FMT from the IPC tablet (DCF-NF:HA=1:1) was slower than that from a SDCH-NF only tablet.
26501272	3	60	theme	radical	685:691	arg1	scavengers					693:702	relatively efficient radical scavengers	664:702	relatively efficient radical scavengers	664:702	SDCH-NF and HA had acceptable scavenging abilities and were relatively efficient radical scavengers, but UCH was much less effective.
26501272	4	61	theme	compounds	807:815	arg1	HA					775:776	HA	775:776	HA	775:776	The results suggest that SDCH-NF and HA could serve as scavengers of compounds related to the development of oxidative stress.
26501272	4	61	theme	compounds	807:815	arg1	SDCH-NF					763:769	SDCH-NF	763:769	SDCH-NF	763:769	The results suggest that SDCH-NF and HA could serve as scavengers of compounds related to the development of oxidative stress.
26501272	4	61	theme	compounds	807:815	arg1	scavengers					793:802	scavengers	793:802	scavengers of compounds related to the development of oxidative stress	793:862	The results suggest that SDCH-NF and HA could serve as scavengers of compounds related to the development of oxidative stress.
26501272	1	62	theme	hyaluronic	247:256	arg1	HA					264:265	HA	264:265	HA	264:265	In this study, we examined a possible use of a surface-deacetylated chitin nano-fiber (SDCH-NF) and hyaluronic acid (HA) interpolymer complex (IPC) tablet as a potential antioxidative compound in extended-release matrix tablets.
26501272	1	62	theme	hyaluronic	247:256	arg1	acid					258:261	hyaluronic acid	247:261	hyaluronic acid (HA)	247:266	In this study, we examined a possible use of a surface-deacetylated chitin nano-fiber (SDCH-NF) and hyaluronic acid (HA) interpolymer complex (IPC) tablet as a potential antioxidative compound in extended-release matrix tablets.
26501272	7	63	theme	optimum	1178:1184	arg1	ratio					1199:1203	the optimum complexation ratio	1174:1203	the optimum complexation ratio for IPC between SDCH-NF/HA	1174:1230	Turbidity measurements and X-ray diffraction (XRD) data also indicated that the optimum complexation ratio for IPC between SDCH-NF/HA is 1/1, resulting in a good relationship between turbidity or XRD of the complex and the release ratio of FMT.
26501272	7	63	theme	optimum	1178:1184	arg1	1/1					1235:1237	1/1	1235:1237	1/1	1235:1237	Turbidity measurements and X-ray diffraction (XRD) data also indicated that the optimum complexation ratio for IPC between SDCH-NF/HA is 1/1, resulting in a good relationship between turbidity or XRD of the complex and the release ratio of FMT.
26501272	1	64	theme	antioxidative	317:329	arg1	compound					331:338	a potential antioxidative compound	305:338	a potential antioxidative compound in extended-release matrix tablets	305:373	In this study, we examined a possible use of a surface-deacetylated chitin nano-fiber (SDCH-NF) and hyaluronic acid (HA) interpolymer complex (IPC) tablet as a potential antioxidative compound in extended-release matrix tablets.
26501272	1	65	dep	nano-fiber	222:231	arg1	tablet					295:300	interpolymer complex (IPC) tablet	268:300	interpolymer complex (IPC) tablet	268:300	In this study, we examined a possible use of a surface-deacetylated chitin nano-fiber (SDCH-NF) and hyaluronic acid (HA) interpolymer complex (IPC) tablet as a potential antioxidative compound in extended-release matrix tablets.
26501272	7	66	theme	diffraction	1131:1141	arg1	data					1149:1152	X-ray diffraction (XRD) data	1125:1152	X-ray diffraction (XRD) data	1125:1152	Turbidity measurements and X-ray diffraction (XRD) data also indicated that the optimum complexation ratio for IPC between SDCH-NF/HA is 1/1, resulting in a good relationship between turbidity or XRD of the complex and the release ratio of FMT.
26501272	1	67	theme	acid	258:261	arg1	use					185:187	a possible use	174:187	a possible use of a surface-deacetylated chitin nano-fiber (SDCH-NF) and hyaluronic acid (HA) interpolymer complex (IPC) tablet as a potential antioxidative compound in extended-release matrix tablets	174:373	In this study, we examined a possible use of a surface-deacetylated chitin nano-fiber (SDCH-NF) and hyaluronic acid (HA) interpolymer complex (IPC) tablet as a potential antioxidative compound in extended-release matrix tablets.
24956839	0	0	theme	release	81:87	arg1	unit					89:92	micro pill drug release unit	65:92	[Study on preparation of ginkgolides component solid dispersions micro pill drug release unit	0:92	[Study on preparation of ginkgolides component solid dispersions micro pill drug release unit].
24956839	4	1	theme	principle	548:556	arg1	agent					558:562	principle agent	548:562	principle agent	548:562	Ginkgolides component solid dispersions as principle agent were prepared for micro-pellet.
24956839	5	2	theme	micro-pellet	843:854	arg1	unit					869:872	micro-pellet drug release unit	843:872	micro-pellet drug release unit	843:872	Comparison of different types, different doses of the adhesive, drug-polymer interactions, and disintegrating agent for the preparation of ginkgolides components of micro-pellet drug release unit, the optimum preparation ginkgolides components of micro-pellet drug release unit was screened by orthogonal design experiment.
24956839	5	3	theme	micro-pellet	761:772	arg1	ginkgolides					817:827	the optimum preparation ginkgolides	793:827	the optimum preparation ginkgolides	793:827	Comparison of different types, different doses of the adhesive, drug-polymer interactions, and disintegrating agent for the preparation of ginkgolides components of micro-pellet drug release unit, the optimum preparation ginkgolides components of micro-pellet drug release unit was screened by orthogonal design experiment.
24956839	5	3	theme	micro-pellet	761:772	arg1	unit					787:790	micro-pellet drug release unit	761:790	micro-pellet drug release unit	761:790	Comparison of different types, different doses of the adhesive, drug-polymer interactions, and disintegrating agent for the preparation of ginkgolides components of micro-pellet drug release unit, the optimum preparation ginkgolides components of micro-pellet drug release unit was screened by orthogonal design experiment.
24956839	2	4	theme	ginkgolides	357:367	arg1	dispersions					385:395	ginkgolides component solid dispersions	357:395	ginkgolides component solid dispersions	357:395	Microcrystalline cellulose, chitosan as composite carrier, solvent method was used to prepare ginkgolides component solid dispersions.
24956839	6	5	dep	ratio	1024:1028	arg1	3					1033:1033	3	1033:1033	3	1033:1033	Preparation of ginkgolides components solid dispersions with microcrystalline cellulose and chitosan at ratio 1: 3.
24956839	0	6	theme	drug	76:79	arg1	unit					89:92	micro pill drug release unit	65:92	[Study on preparation of ginkgolides component solid dispersions micro pill drug release unit	0:92	[Study on preparation of ginkgolides component solid dispersions micro pill drug release unit].
24956839	8	7	theme	drug	1123:1126	arg1	unit					1136:1139	Solid dispersion-micro-pellet drug release unit	1093:1139	Solid dispersion-micro-pellet drug release unit	1093:1139	Solid dispersion-micro-pellet drug release unit can significantly improve the dissolution of ginkgolides components, it has practical application value.
24956839	5	8	theme	adhesive	650:657	arg1	interactions					673:684	the adhesive, drug-polymer interactions	646:684	interactions	673:684	Comparison of different types, different doses of the adhesive, drug-polymer interactions, and disintegrating agent for the preparation of ginkgolides components of micro-pellet drug release unit, the optimum preparation ginkgolides components of micro-pellet drug release unit was screened by orthogonal design experiment.
24956839	1	9	theme	ginkgolides	160:170	arg1	dispersions					188:198	ginkgolides component solid dispersions micro pill drug release unit	160:227	ginkgolides component solid dispersions micro pill drug release unit	160:227	Microcrystalline cellulose and chitosan were applied to prepare ginkgolides component solid dispersions micro pill drug release unit and study the dissolution of GKS.
24956839	6	10	theme	solid	958:962	arg1	dispersions					964:974	solid dispersions	958:974	solid dispersions	958:974	Preparation of ginkgolides components solid dispersions with microcrystalline cellulose and chitosan at ratio 1: 3.
24956839	1	11	theme	GKS	258:260	arg1	dissolution					243:253	the dissolution	239:253	the dissolution of GKS	239:260	Microcrystalline cellulose and chitosan were applied to prepare ginkgolides component solid dispersions micro pill drug release unit and study the dissolution of GKS.
24956839	0	12	from	[Study	0:5	arg1	preparation					10:20	preparation	10:20	preparation of ginkgolides component solid dispersions	10:63	[Study on preparation of ginkgolides component solid dispersions micro pill drug release unit].
24956839	2	13	theme	composite	303:311	arg1	carrier					313:319	composite carrier	303:319	composite carrier	303:319	Microcrystalline cellulose, chitosan as composite carrier, solvent method was used to prepare ginkgolides component solid dispersions.
24956839	5	14	theme	optimum	797:803	arg1	ginkgolides					817:827	the optimum preparation ginkgolides	793:827	the optimum preparation ginkgolides	793:827	Comparison of different types, different doses of the adhesive, drug-polymer interactions, and disintegrating agent for the preparation of ginkgolides components of micro-pellet drug release unit, the optimum preparation ginkgolides components of micro-pellet drug release unit was screened by orthogonal design experiment.
24956839	5	14	theme	optimum	797:803	arg1	unit					787:790	micro-pellet drug release unit	761:790	micro-pellet drug release unit	761:790	Comparison of different types, different doses of the adhesive, drug-polymer interactions, and disintegrating agent for the preparation of ginkgolides components of micro-pellet drug release unit, the optimum preparation ginkgolides components of micro-pellet drug release unit was screened by orthogonal design experiment.
24956839	4	15	theme	solid	527:531	arg1	dispersions					533:543	Ginkgolides component solid dispersions	505:543	Ginkgolides component solid dispersions as principle agent	505:562	Ginkgolides component solid dispersions as principle agent were prepared for micro-pellet.
24956839	5	16	theme	drug-polymer	660:671	arg1	interactions					673:684	the adhesive, drug-polymer interactions	646:684	interactions	673:684	Comparison of different types, different doses of the adhesive, drug-polymer interactions, and disintegrating agent for the preparation of ginkgolides components of micro-pellet drug release unit, the optimum preparation ginkgolides components of micro-pellet drug release unit was screened by orthogonal design experiment.
24956839	6	17	with	components	947:956	arg1	chitosan					1012:1019	chitosan	1012:1019	chitosan	1012:1019	Preparation of ginkgolides components solid dispersions with microcrystalline cellulose and chitosan at ratio 1: 3.
24956839	6	17	with	components	947:956	arg1	cellulose					998:1006	microcrystalline cellulose	981:1006	microcrystalline cellulose	981:1006	Preparation of ginkgolides components solid dispersions with microcrystalline cellulose and chitosan at ratio 1: 3.
24956839	6	18	from	ratio	1024:1028	arg1	components					947:956	components	947:956	components	947:956	Preparation of ginkgolides components solid dispersions with microcrystalline cellulose and chitosan at ratio 1: 3.
24956839	5	19	theme	preparation	805:815	arg1	ginkgolides					817:827	the optimum preparation ginkgolides	793:827	the optimum preparation ginkgolides	793:827	Comparison of different types, different doses of the adhesive, drug-polymer interactions, and disintegrating agent for the preparation of ginkgolides components of micro-pellet drug release unit, the optimum preparation ginkgolides components of micro-pellet drug release unit was screened by orthogonal design experiment.
24956839	5	19	theme	preparation	805:815	arg1	unit					787:790	micro-pellet drug release unit	761:790	micro-pellet drug release unit	761:790	Comparison of different types, different doses of the adhesive, drug-polymer interactions, and disintegrating agent for the preparation of ginkgolides components of micro-pellet drug release unit, the optimum preparation ginkgolides components of micro-pellet drug release unit was screened by orthogonal design experiment.
24956839	4	20	theme	component	517:525	arg1	dispersions					533:543	Ginkgolides component solid dispersions	505:543	Ginkgolides component solid dispersions as principle agent	505:562	Ginkgolides component solid dispersions as principle agent were prepared for micro-pellet.
24956839	5	21	theme	interactions	673:684	arg1	agent					706:710	disintegrating agent	691:710	disintegrating agent for the preparation of ginkgolides components of micro-pellet drug release unit, the optimum preparation ginkgolides components of micro-pellet drug release unit	691:872	Comparison of different types, different doses of the adhesive, drug-polymer interactions, and disintegrating agent for the preparation of ginkgolides components of micro-pellet drug release unit, the optimum preparation ginkgolides components of micro-pellet drug release unit was screened by orthogonal design experiment.
24956839	5	21	theme	interactions	673:684	arg1	Comparison					596:605	Comparison	596:605	Comparison of different types	596:624	Comparison of different types, different doses of the adhesive, drug-polymer interactions, and disintegrating agent for the preparation of ginkgolides components of micro-pellet drug release unit, the optimum preparation ginkgolides components of micro-pellet drug release unit was screened by orthogonal design experiment.
24956839	5	21	theme	interactions	673:684	arg1	doses					637:641	different doses	627:641	different doses of the adhesive, drug-polymer interactions	627:684	Comparison of different types, different doses of the adhesive, drug-polymer interactions, and disintegrating agent for the preparation of ginkgolides components of micro-pellet drug release unit, the optimum preparation ginkgolides components of micro-pellet drug release unit was screened by orthogonal design experiment.
24956839	1	22	theme	component	172:180	arg1	dispersions					188:198	ginkgolides component solid dispersions micro pill drug release unit	160:227	ginkgolides component solid dispersions micro pill drug release unit	160:227	Microcrystalline cellulose and chitosan were applied to prepare ginkgolides component solid dispersions micro pill drug release unit and study the dissolution of GKS.
24956839	5	23	theme	release	861:867	arg1	unit					869:872	micro-pellet drug release unit	843:872	micro-pellet drug release unit	843:872	Comparison of different types, different doses of the adhesive, drug-polymer interactions, and disintegrating agent for the preparation of ginkgolides components of micro-pellet drug release unit, the optimum preparation ginkgolides components of micro-pellet drug release unit was screened by orthogonal design experiment.
24956839	5	24	theme	different	627:635	arg1	doses					637:641	different doses	627:641	different doses of the adhesive, drug-polymer interactions	627:684	Comparison of different types, different doses of the adhesive, drug-polymer interactions, and disintegrating agent for the preparation of ginkgolides components of micro-pellet drug release unit, the optimum preparation ginkgolides components of micro-pellet drug release unit was screened by orthogonal design experiment.
24956839	5	25	theme	orthogonal	890:899	arg1	experiment					908:917	orthogonal design experiment	890:917	orthogonal design experiment	890:917	Comparison of different types, different doses of the adhesive, drug-polymer interactions, and disintegrating agent for the preparation of ginkgolides components of micro-pellet drug release unit, the optimum preparation ginkgolides components of micro-pellet drug release unit was screened by orthogonal design experiment.
24956839	1	26	theme	solid	182:186	arg1	dispersions					188:198	ginkgolides component solid dispersions micro pill drug release unit	160:227	ginkgolides component solid dispersions micro pill drug release unit	160:227	Microcrystalline cellulose and chitosan were applied to prepare ginkgolides component solid dispersions micro pill drug release unit and study the dissolution of GKS.
24956839	3	27	theme	Differential	398:409	arg1	calorimetry					420:430	Differential scanning calorimetry	398:430	Differential scanning calorimetry	398:430	Differential scanning calorimetry was used to Characterization of ginkgolides component solid dispersions.
24956839	5	28	theme	drug	856:859	arg1	unit					869:872	micro-pellet drug release unit	843:872	micro-pellet drug release unit	843:872	Comparison of different types, different doses of the adhesive, drug-polymer interactions, and disintegrating agent for the preparation of ginkgolides components of micro-pellet drug release unit, the optimum preparation ginkgolides components of micro-pellet drug release unit was screened by orthogonal design experiment.
24956839	5	29	theme	disintegrating	691:704	arg1	agent					706:710	disintegrating agent	691:710	disintegrating agent for the preparation of ginkgolides components of micro-pellet drug release unit, the optimum preparation ginkgolides components of micro-pellet drug release unit	691:872	Comparison of different types, different doses of the adhesive, drug-polymer interactions, and disintegrating agent for the preparation of ginkgolides components of micro-pellet drug release unit, the optimum preparation ginkgolides components of micro-pellet drug release unit was screened by orthogonal design experiment.
24956839	5	30	theme	components	829:838	arg1	components					747:756	ginkgolides components	735:756	ginkgolides components of micro-pellet drug release unit, the optimum preparation ginkgolides components of micro-pellet drug release unit	735:872	Comparison of different types, different doses of the adhesive, drug-polymer interactions, and disintegrating agent for the preparation of ginkgolides components of micro-pellet drug release unit, the optimum preparation ginkgolides components of micro-pellet drug release unit was screened by orthogonal design experiment.
24956839	2	31	theme	solvent	322:328	arg1	method					330:335	solvent method	322:335	solvent method	322:335	Microcrystalline cellulose, chitosan as composite carrier, solvent method was used to prepare ginkgolides component solid dispersions.
24956839	2	31	theme	solvent	322:328	arg1	cellulose					280:288	Microcrystalline cellulose	263:288	Microcrystalline cellulose	263:288	Microcrystalline cellulose, chitosan as composite carrier, solvent method was used to prepare ginkgolides component solid dispersions.
24956839	8	32	theme	ginkgolides	1186:1196	arg1	components					1198:1207	ginkgolides components	1186:1207	ginkgolides components	1186:1207	Solid dispersion-micro-pellet drug release unit can significantly improve the dissolution of ginkgolides components, it has practical application value.
24956839	3	33	theme	scanning	411:418	arg1	calorimetry					420:430	Differential scanning calorimetry	398:430	Differential scanning calorimetry	398:430	Differential scanning calorimetry was used to Characterization of ginkgolides component solid dispersions.
24956839	2	34	used	used	341:344	arg2	method					330:335	solvent method	322:335	solvent method	322:335	Microcrystalline cellulose, chitosan as composite carrier, solvent method was used to prepare ginkgolides component solid dispersions.
24956839	2	34	used	used	341:344	arg2	cellulose					280:288	Microcrystalline cellulose	263:288	Microcrystalline cellulose	263:288	Microcrystalline cellulose, chitosan as composite carrier, solvent method was used to prepare ginkgolides component solid dispersions.
24956839	2	34	used	used	341:344	arg2	chitosan					291:298	chitosan	291:298	chitosan as composite carrier	291:319	Microcrystalline cellulose, chitosan as composite carrier, solvent method was used to prepare ginkgolides component solid dispersions.
24956839	1	35	theme	micro	200:204	arg1	unit					224:227	micro pill drug release unit	200:227	ginkgolides component solid dispersions micro pill drug release unit	160:227	Microcrystalline cellulose and chitosan were applied to prepare ginkgolides component solid dispersions micro pill drug release unit and study the dissolution of GKS.
24956839	5	36	theme	design	901:906	arg1	experiment					908:917	orthogonal design experiment	890:917	orthogonal design experiment	890:917	Comparison of different types, different doses of the adhesive, drug-polymer interactions, and disintegrating agent for the preparation of ginkgolides components of micro-pellet drug release unit, the optimum preparation ginkgolides components of micro-pellet drug release unit was screened by orthogonal design experiment.
24956839	8	37	theme	dispersion-micro-pellet	1099:1121	arg1	unit					1136:1139	Solid dispersion-micro-pellet drug release unit	1093:1139	Solid dispersion-micro-pellet drug release unit	1093:1139	Solid dispersion-micro-pellet drug release unit can significantly improve the dissolution of ginkgolides components, it has practical application value.
24956839	5	38	theme	drug	774:777	arg1	ginkgolides					817:827	the optimum preparation ginkgolides	793:827	the optimum preparation ginkgolides	793:827	Comparison of different types, different doses of the adhesive, drug-polymer interactions, and disintegrating agent for the preparation of ginkgolides components of micro-pellet drug release unit, the optimum preparation ginkgolides components of micro-pellet drug release unit was screened by orthogonal design experiment.
24956839	5	38	theme	drug	774:777	arg1	unit					787:790	micro-pellet drug release unit	761:790	micro-pellet drug release unit	761:790	Comparison of different types, different doses of the adhesive, drug-polymer interactions, and disintegrating agent for the preparation of ginkgolides components of micro-pellet drug release unit, the optimum preparation ginkgolides components of micro-pellet drug release unit was screened by orthogonal design experiment.
24956839	8	39	theme	Solid	1093:1097	arg1	unit					1136:1139	Solid dispersion-micro-pellet drug release unit	1093:1139	Solid dispersion-micro-pellet drug release unit	1093:1139	Solid dispersion-micro-pellet drug release unit can significantly improve the dissolution of ginkgolides components, it has practical application value.
24956839	1	40	theme	pill	206:209	arg1	unit					224:227	micro pill drug release unit	200:227	ginkgolides component solid dispersions micro pill drug release unit	160:227	Microcrystalline cellulose and chitosan were applied to prepare ginkgolides component solid dispersions micro pill drug release unit and study the dissolution of GKS.
24956839	5	41	theme	release	779:785	arg1	ginkgolides					817:827	the optimum preparation ginkgolides	793:827	the optimum preparation ginkgolides	793:827	Comparison of different types, different doses of the adhesive, drug-polymer interactions, and disintegrating agent for the preparation of ginkgolides components of micro-pellet drug release unit, the optimum preparation ginkgolides components of micro-pellet drug release unit was screened by orthogonal design experiment.
24956839	5	41	theme	release	779:785	arg1	unit					787:790	micro-pellet drug release unit	761:790	micro-pellet drug release unit	761:790	Comparison of different types, different doses of the adhesive, drug-polymer interactions, and disintegrating agent for the preparation of ginkgolides components of micro-pellet drug release unit, the optimum preparation ginkgolides components of micro-pellet drug release unit was screened by orthogonal design experiment.
24956839	1	42	theme	Microcrystalline	96:111	arg1	cellulose					113:121	Microcrystalline cellulose	96:121	Microcrystalline cellulose	96:121	Microcrystalline cellulose and chitosan were applied to prepare ginkgolides component solid dispersions micro pill drug release unit and study the dissolution of GKS.
24956839	1	43	theme	drug	211:214	arg1	unit					224:227	micro pill drug release unit	200:227	ginkgolides component solid dispersions micro pill drug release unit	160:227	Microcrystalline cellulose and chitosan were applied to prepare ginkgolides component solid dispersions micro pill drug release unit and study the dissolution of GKS.
24956839	0	44	theme	component	37:45	arg1	dispersions					53:63	ginkgolides component solid dispersions	25:63	ginkgolides component solid dispersions	25:63	[Study on preparation of ginkgolides component solid dispersions micro pill drug release unit].
24956839	5	45	theme	unit	787:790	arg1	components					829:838	micro-pellet drug release unit, the optimum preparation ginkgolides components	761:838	micro-pellet drug release unit, the optimum preparation ginkgolides components of micro-pellet drug release unit	761:872	Comparison of different types, different doses of the adhesive, drug-polymer interactions, and disintegrating agent for the preparation of ginkgolides components of micro-pellet drug release unit, the optimum preparation ginkgolides components of micro-pellet drug release unit was screened by orthogonal design experiment.
24956839	3	46	theme	solid	486:490	arg1	dispersions					492:502	ginkgolides component solid dispersions	464:502	ginkgolides component solid dispersions	464:502	Differential scanning calorimetry was used to Characterization of ginkgolides component solid dispersions.
24956839	1	47	dep	dispersions	188:198	arg1	unit					224:227	micro pill drug release unit	200:227	ginkgolides component solid dispersions micro pill drug release unit	160:227	Microcrystalline cellulose and chitosan were applied to prepare ginkgolides component solid dispersions micro pill drug release unit and study the dissolution of GKS.
24956839	1	48	theme	release	216:222	arg1	unit					224:227	micro pill drug release unit	200:227	ginkgolides component solid dispersions micro pill drug release unit	160:227	Microcrystalline cellulose and chitosan were applied to prepare ginkgolides component solid dispersions micro pill drug release unit and study the dissolution of GKS.
24956839	0	49	theme	ginkgolides	25:35	arg1	dispersions					53:63	ginkgolides component solid dispersions	25:63	ginkgolides component solid dispersions	25:63	[Study on preparation of ginkgolides component solid dispersions micro pill drug release unit].
24956839	5	50	theme	unit	869:872	arg1	components					829:838	micro-pellet drug release unit, the optimum preparation ginkgolides components	761:838	micro-pellet drug release unit, the optimum preparation ginkgolides components of micro-pellet drug release unit	761:872	Comparison of different types, different doses of the adhesive, drug-polymer interactions, and disintegrating agent for the preparation of ginkgolides components of micro-pellet drug release unit, the optimum preparation ginkgolides components of micro-pellet drug release unit was screened by orthogonal design experiment.
24956839	4	51	theme	Ginkgolides	505:515	arg1	dispersions					533:543	Ginkgolides component solid dispersions	505:543	Ginkgolides component solid dispersions as principle agent	505:562	Ginkgolides component solid dispersions as principle agent were prepared for micro-pellet.
24956839	3	52	theme	dispersions	492:502	arg1	Characterization					444:459	Characterization	444:459	Characterization of ginkgolides component solid dispersions	444:502	Differential scanning calorimetry was used to Characterization of ginkgolides component solid dispersions.
24956839	0	53	theme	dispersions	53:63	arg1	preparation					10:20	preparation	10:20	preparation of ginkgolides component solid dispersions	10:63	[Study on preparation of ginkgolides component solid dispersions micro pill drug release unit].
24956839	5	54	theme	ginkgolides	735:745	arg1	components					747:756	ginkgolides components	735:756	ginkgolides components of micro-pellet drug release unit, the optimum preparation ginkgolides components of micro-pellet drug release unit	735:872	Comparison of different types, different doses of the adhesive, drug-polymer interactions, and disintegrating agent for the preparation of ginkgolides components of micro-pellet drug release unit, the optimum preparation ginkgolides components of micro-pellet drug release unit was screened by orthogonal design experiment.
24956839	3	55	theme	ginkgolides	464:474	arg1	dispersions					492:502	ginkgolides component solid dispersions	464:502	ginkgolides component solid dispersions	464:502	Differential scanning calorimetry was used to Characterization of ginkgolides component solid dispersions.
24956839	5	56	theme	different	610:618	arg1	types					620:624	different types	610:624	different types	610:624	Comparison of different types, different doses of the adhesive, drug-polymer interactions, and disintegrating agent for the preparation of ginkgolides components of micro-pellet drug release unit, the optimum preparation ginkgolides components of micro-pellet drug release unit was screened by orthogonal design experiment.
24956839	0	57	theme	solid	47:51	arg1	dispersions					53:63	ginkgolides component solid dispersions	25:63	ginkgolides component solid dispersions	25:63	[Study on preparation of ginkgolides component solid dispersions micro pill drug release unit].
24956839	5	58	theme	components	747:756	arg1	preparation					720:730	the preparation	716:730	the preparation of ginkgolides components of micro-pellet drug release unit, the optimum preparation ginkgolides components of micro-pellet drug release unit	716:872	Comparison of different types, different doses of the adhesive, drug-polymer interactions, and disintegrating agent for the preparation of ginkgolides components of micro-pellet drug release unit, the optimum preparation ginkgolides components of micro-pellet drug release unit was screened by orthogonal design experiment.
24956839	6	59	theme	ginkgolides	935:945	arg1	Preparation					920:930	Preparation	920:930	Preparation of ginkgolides	920:945	Preparation of ginkgolides components solid dispersions with microcrystalline cellulose and chitosan at ratio 1: 3.
24956839	5	60	theme	types	620:624	arg1	agent					706:710	disintegrating agent	691:710	disintegrating agent for the preparation of ginkgolides components of micro-pellet drug release unit, the optimum preparation ginkgolides components of micro-pellet drug release unit	691:872	Comparison of different types, different doses of the adhesive, drug-polymer interactions, and disintegrating agent for the preparation of ginkgolides components of micro-pellet drug release unit, the optimum preparation ginkgolides components of micro-pellet drug release unit was screened by orthogonal design experiment.
24956839	5	60	theme	types	620:624	arg1	Comparison					596:605	Comparison	596:605	Comparison of different types	596:624	Comparison of different types, different doses of the adhesive, drug-polymer interactions, and disintegrating agent for the preparation of ginkgolides components of micro-pellet drug release unit, the optimum preparation ginkgolides components of micro-pellet drug release unit was screened by orthogonal design experiment.
24956839	5	60	theme	types	620:624	arg1	doses					637:641	different doses	627:641	different doses of the adhesive, drug-polymer interactions	627:684	Comparison of different types, different doses of the adhesive, drug-polymer interactions, and disintegrating agent for the preparation of ginkgolides components of micro-pellet drug release unit, the optimum preparation ginkgolides components of micro-pellet drug release unit was screened by orthogonal design experiment.
24956839	6	61	theme	microcrystalline	981:996	arg1	cellulose					998:1006	microcrystalline cellulose	981:1006	microcrystalline cellulose	981:1006	Preparation of ginkgolides components solid dispersions with microcrystalline cellulose and chitosan at ratio 1: 3.
24956839	8	62	theme	application	1227:1237	arg1	value					1239:1243	practical application value	1217:1243	practical application value	1217:1243	Solid dispersion-micro-pellet drug release unit can significantly improve the dissolution of ginkgolides components, it has practical application value.
24956839	7	63	theme	Drug	1036:1039	arg1	dissolution					1052:1062	Drug cumulative dissolution	1036:1062	Drug cumulative dissolution	1036:1062	Drug cumulative dissolution was more than 80% in 60 min.
24956839	2	64	theme	Microcrystalline	263:278	arg1	chitosan					291:298	chitosan	291:298	chitosan as composite carrier	291:319	Microcrystalline cellulose, chitosan as composite carrier, solvent method was used to prepare ginkgolides component solid dispersions.
24956839	2	64	theme	Microcrystalline	263:278	arg1	cellulose					280:288	Microcrystalline cellulose	263:288	Microcrystalline cellulose	263:288	Microcrystalline cellulose, chitosan as composite carrier, solvent method was used to prepare ginkgolides component solid dispersions.
24956839	2	64	theme	Microcrystalline	263:278	arg1	method					330:335	solvent method	322:335	solvent method	322:335	Microcrystalline cellulose, chitosan as composite carrier, solvent method was used to prepare ginkgolides component solid dispersions.
24956839	8	65	theme	practical	1217:1225	arg1	value					1239:1243	practical application value	1217:1243	practical application value	1217:1243	Solid dispersion-micro-pellet drug release unit can significantly improve the dissolution of ginkgolides components, it has practical application value.
24956839	3	66	used	used	436:439	arg2	calorimetry					420:430	Differential scanning calorimetry	398:430	Differential scanning calorimetry	398:430	Differential scanning calorimetry was used to Characterization of ginkgolides component solid dispersions.
24956839	8	67	contain	has	1213:1215	arg2	value					1239:1243	practical application value	1217:1243	practical application value	1217:1243	Solid dispersion-micro-pellet drug release unit can significantly improve the dissolution of ginkgolides components, it has practical application value.
24956839	8	67	contain	has	1213:1215	arg1	it					1210:1211	it	1210:1211	it	1210:1211	Solid dispersion-micro-pellet drug release unit can significantly improve the dissolution of ginkgolides components, it has practical application value.
24956839	0	68	theme	pill	71:74	arg1	unit					89:92	micro pill drug release unit	65:92	[Study on preparation of ginkgolides component solid dispersions micro pill drug release unit	0:92	[Study on preparation of ginkgolides component solid dispersions micro pill drug release unit].
24956839	8	69	theme	release	1128:1134	arg1	unit					1136:1139	Solid dispersion-micro-pellet drug release unit	1093:1139	Solid dispersion-micro-pellet drug release unit	1093:1139	Solid dispersion-micro-pellet drug release unit can significantly improve the dissolution of ginkgolides components, it has practical application value.
24956839	2	70	theme	solid	379:383	arg1	dispersions					385:395	ginkgolides component solid dispersions	357:395	ginkgolides component solid dispersions	357:395	Microcrystalline cellulose, chitosan as composite carrier, solvent method was used to prepare ginkgolides component solid dispersions.
24956839	3	71	theme	component	476:484	arg1	dispersions					492:502	ginkgolides component solid dispersions	464:502	ginkgolides component solid dispersions	464:502	Differential scanning calorimetry was used to Characterization of ginkgolides component solid dispersions.
24956839	7	72	from	%	1080:1080	arg1	min					1088:1090	60 min	1085:1090	60 min	1085:1090	Drug cumulative dissolution was more than 80% in 60 min.
24956839	7	73	theme	cumulative	1041:1050	arg1	dissolution					1052:1062	Drug cumulative dissolution	1036:1062	Drug cumulative dissolution	1036:1062	Drug cumulative dissolution was more than 80% in 60 min.
24956839	0	74	dep	[Study	0:5	arg1	unit					89:92	micro pill drug release unit	65:92	[Study on preparation of ginkgolides component solid dispersions micro pill drug release unit	0:92	[Study on preparation of ginkgolides component solid dispersions micro pill drug release unit].
24956839	2	75	theme	component	369:377	arg1	dispersions					385:395	ginkgolides component solid dispersions	357:395	ginkgolides component solid dispersions	357:395	Microcrystalline cellulose, chitosan as composite carrier, solvent method was used to prepare ginkgolides component solid dispersions.
24956839	8	76	theme	components	1198:1207	arg1	dissolution					1171:1181	the dissolution	1167:1181	the dissolution of ginkgolides components	1167:1207	Solid dispersion-micro-pellet drug release unit can significantly improve the dissolution of ginkgolides components, it has practical application value.
28161851	0	0	theme	Multiple	84:91	arg1	Polysaccharides					93:107	Multiple Polysaccharides	84:107	Multiple Polysaccharides	84:107	Genome Sequence of Microbulbifer mangrovi DD-13T Reveals Its Versatility to Degrade Multiple Polysaccharides.
28161851	10	1	theme	concomitant	1768:1778	arg1	production					1780:1789	concomitant production	1768:1789	concomitant production of homologous carbohydrase	1768:1816	Additionally, several other polysaccharides such as carrageenan, xylan, pullulan, pectin, starch, and carboxymethyl cellulose were also corroborated as growth substrate for strain DD-13 and were associated with concomitant production of homologous carbohydrase.
28161851	5	2	theme	Carbohydrate-Active	611:629	arg1	Toolkit					658:664	a Carbohydrate-Active Enzymes (CAZymes) Analysis Toolkit	609:664	a Carbohydrate-Active Enzymes (CAZymes) Analysis Toolkit	609:664	Analysis of nucleotide sequence of predicted gene using a Carbohydrate-Active Enzymes (CAZymes) Analysis Toolkit indicates that strain DD-13 encodes a large set of CAZymes including 255 glycoside hydrolases, 76 carbohydrate esterases, 17 polysaccharide lyases, and 113 carbohydrate-binding modules (CBMs).
28161851	9	3	theme	sugars	1549:1554	arg1	production					1512:1521	the production	1508:1521	the production of metabolizable reducing sugars	1508:1554	The homologous carbohydrase produced by strain DD-13 during growth degraded the polysaccharide, ensuring the production of metabolizable reducing sugars.
28161851	7	4	theme	strain	1173:1178	arg1	versatility					1158:1168	the versatility	1154:1168	the versatility of strain DD-13 to degrade complex polysaccharides	1154:1219	Some of polysaccharide-degrading genes were highly modular and were appended at least with one CBM indicating the versatility of strain DD-13 to degrade complex polysaccharides.
28161851	5	5	theme	glycoside	739:747	arg1	hydrolases					749:758	255 glycoside hydrolases	735:758	255 glycoside hydrolases	735:758	Analysis of nucleotide sequence of predicted gene using a Carbohydrate-Active Enzymes (CAZymes) Analysis Toolkit indicates that strain DD-13 encodes a large set of CAZymes including 255 glycoside hydrolases, 76 carbohydrate esterases, 17 polysaccharide lyases, and 113 carbohydrate-binding modules (CBMs).
28161851	5	6	theme	Enzymes	631:637	arg1	Toolkit					658:664	a Carbohydrate-Active Enzymes (CAZymes) Analysis Toolkit	609:664	a Carbohydrate-Active Enzymes (CAZymes) Analysis Toolkit	609:664	Analysis of nucleotide sequence of predicted gene using a Carbohydrate-Active Enzymes (CAZymes) Analysis Toolkit indicates that strain DD-13 encodes a large set of CAZymes including 255 glycoside hydrolases, 76 carbohydrate esterases, 17 polysaccharide lyases, and 113 carbohydrate-binding modules (CBMs).
28161851	10	7	theme	homologous	1794:1803	arg1	carbohydrase					1805:1816	homologous carbohydrase	1794:1816	homologous carbohydrase	1794:1816	Additionally, several other polysaccharides such as carrageenan, xylan, pullulan, pectin, starch, and carboxymethyl cellulose were also corroborated as growth substrate for strain DD-13 and were associated with concomitant production of homologous carbohydrase.
28161851	9	8	theme	homologous	1407:1416	arg1	carbohydrase					1418:1429	The homologous carbohydrase	1403:1429	The homologous carbohydrase produced by strain DD-13 during growth	1403:1468	The homologous carbohydrase produced by strain DD-13 during growth degraded the polysaccharide, ensuring the production of metabolizable reducing sugars.
28161851	4	9	theme	coding	489:494	arg1	sequences					496:504	protein coding sequences	481:504	protein coding sequences	481:504	In addition to protein coding sequences, 34 tRNA genes and 3 rRNA genes were detected.
28161851	5	10	theme	nucleotide	565:574	arg1	sequence					576:583	nucleotide sequence	565:583	nucleotide sequence of predicted gene	565:601	Analysis of nucleotide sequence of predicted gene using a Carbohydrate-Active Enzymes (CAZymes) Analysis Toolkit indicates that strain DD-13 encodes a large set of CAZymes including 255 glycoside hydrolases, 76 carbohydrate esterases, 17 polysaccharide lyases, and 113 carbohydrate-binding modules (CBMs).
28161851	10	11	theme	growth	1709:1714	arg1	polysaccharides					1585:1599	several other polysaccharides	1571:1599	several other polysaccharides such as carrageenan, xylan, pullulan, pectin, starch, and carboxymethyl cellulose	1571:1681	Additionally, several other polysaccharides such as carrageenan, xylan, pullulan, pectin, starch, and carboxymethyl cellulose were also corroborated as growth substrate for strain DD-13 and were associated with concomitant production of homologous carbohydrase.
28161851	10	11	theme	growth	1709:1714	arg1	cellulose					1673:1681	carboxymethyl cellulose	1659:1681	carboxymethyl cellulose	1659:1681	Additionally, several other polysaccharides such as carrageenan, xylan, pullulan, pectin, starch, and carboxymethyl cellulose were also corroborated as growth substrate for strain DD-13 and were associated with concomitant production of homologous carbohydrase.
28161851	10	11	theme	growth	1709:1714	arg1	substrate					1716:1724	growth substrate	1709:1724	growth substrate for strain DD-13	1709:1741	Additionally, several other polysaccharides such as carrageenan, xylan, pullulan, pectin, starch, and carboxymethyl cellulose were also corroborated as growth substrate for strain DD-13 and were associated with concomitant production of homologous carbohydrase.
28161851	10	11	theme	growth	1709:1714	arg1	carrageenan					1609:1619	carrageenan	1609:1619	carrageenan	1609:1619	Additionally, several other polysaccharides such as carrageenan, xylan, pullulan, pectin, starch, and carboxymethyl cellulose were also corroborated as growth substrate for strain DD-13 and were associated with concomitant production of homologous carbohydrase.
28161851	10	11	theme	growth	1709:1714	arg1	pectin					1639:1644	pectin	1639:1644	pectin	1639:1644	Additionally, several other polysaccharides such as carrageenan, xylan, pullulan, pectin, starch, and carboxymethyl cellulose were also corroborated as growth substrate for strain DD-13 and were associated with concomitant production of homologous carbohydrase.
28161851	10	11	theme	growth	1709:1714	arg1	starch					1647:1652	starch	1647:1652	starch	1647:1652	Additionally, several other polysaccharides such as carrageenan, xylan, pullulan, pectin, starch, and carboxymethyl cellulose were also corroborated as growth substrate for strain DD-13 and were associated with concomitant production of homologous carbohydrase.
28161851	10	11	theme	growth	1709:1714	arg1	xylan					1622:1626	xylan	1622:1626	xylan	1622:1626	Additionally, several other polysaccharides such as carrageenan, xylan, pullulan, pectin, starch, and carboxymethyl cellulose were also corroborated as growth substrate for strain DD-13 and were associated with concomitant production of homologous carbohydrase.
28161851	10	11	theme	growth	1709:1714	arg1	pullulan					1629:1636	pullulan	1629:1636	pullulan	1629:1636	Additionally, several other polysaccharides such as carrageenan, xylan, pullulan, pectin, starch, and carboxymethyl cellulose were also corroborated as growth substrate for strain DD-13 and were associated with concomitant production of homologous carbohydrase.
28161851	5	12	theme	large	704:708	arg1	lyases					806:811	17 polysaccharide lyases	788:811	17 polysaccharide lyases	788:811	Analysis of nucleotide sequence of predicted gene using a Carbohydrate-Active Enzymes (CAZymes) Analysis Toolkit indicates that strain DD-13 encodes a large set of CAZymes including 255 glycoside hydrolases, 76 carbohydrate esterases, 17 polysaccharide lyases, and 113 carbohydrate-binding modules (CBMs).
28161851	5	12	theme	large	704:708	arg1	DD-13					688:692	strain DD-13	681:692	strain DD-13	681:692	Analysis of nucleotide sequence of predicted gene using a Carbohydrate-Active Enzymes (CAZymes) Analysis Toolkit indicates that strain DD-13 encodes a large set of CAZymes including 255 glycoside hydrolases, 76 carbohydrate esterases, 17 polysaccharide lyases, and 113 carbohydrate-binding modules (CBMs).
28161851	5	12	theme	large	704:708	arg1	CAZymes					717:723	CAZymes	717:723	CAZymes including 255 glycoside hydrolases, 76 carbohydrate esterases, 17 polysaccharide lyases, and 113 carbohydrate-binding modules (CBMs)	717:856	Analysis of nucleotide sequence of predicted gene using a Carbohydrate-Active Enzymes (CAZymes) Analysis Toolkit indicates that strain DD-13 encodes a large set of CAZymes including 255 glycoside hydrolases, 76 carbohydrate esterases, 17 polysaccharide lyases, and 113 carbohydrate-binding modules (CBMs).
28161851	5	12	theme	large	704:708	arg1	esterases					777:785	76 carbohydrate esterases	761:785	76 carbohydrate esterases	761:785	Analysis of nucleotide sequence of predicted gene using a Carbohydrate-Active Enzymes (CAZymes) Analysis Toolkit indicates that strain DD-13 encodes a large set of CAZymes including 255 glycoside hydrolases, 76 carbohydrate esterases, 17 polysaccharide lyases, and 113 carbohydrate-binding modules (CBMs).
28161851	5	12	theme	large	704:708	arg1	set					710:712	a large set	702:712	a large set of CAZymes including 255 glycoside hydrolases, 76 carbohydrate esterases, 17 polysaccharide lyases, and 113 carbohydrate-binding modules (CBMs)	702:856	Analysis of nucleotide sequence of predicted gene using a Carbohydrate-Active Enzymes (CAZymes) Analysis Toolkit indicates that strain DD-13 encodes a large set of CAZymes including 255 glycoside hydrolases, 76 carbohydrate esterases, 17 polysaccharide lyases, and 113 carbohydrate-binding modules (CBMs).
28161851	5	12	theme	large	704:708	arg1	modules					843:849	113 carbohydrate-binding modules	818:849	113 carbohydrate-binding modules (CBMs)	818:856	Analysis of nucleotide sequence of predicted gene using a Carbohydrate-Active Enzymes (CAZymes) Analysis Toolkit indicates that strain DD-13 encodes a large set of CAZymes including 255 glycoside hydrolases, 76 carbohydrate esterases, 17 polysaccharide lyases, and 113 carbohydrate-binding modules (CBMs).
28161851	5	12	theme	large	704:708	arg1	hydrolases					749:758	255 glycoside hydrolases	735:758	255 glycoside hydrolases	735:758	Analysis of nucleotide sequence of predicted gene using a Carbohydrate-Active Enzymes (CAZymes) Analysis Toolkit indicates that strain DD-13 encodes a large set of CAZymes including 255 glycoside hydrolases, 76 carbohydrate esterases, 17 polysaccharide lyases, and 113 carbohydrate-binding modules (CBMs).
28161851	5	13	theme	CAZymes	640:646	arg1	Toolkit					658:664	a Carbohydrate-Active Enzymes (CAZymes) Analysis Toolkit	609:664	a Carbohydrate-Active Enzymes (CAZymes) Analysis Toolkit	609:664	Analysis of nucleotide sequence of predicted gene using a Carbohydrate-Active Enzymes (CAZymes) Analysis Toolkit indicates that strain DD-13 encodes a large set of CAZymes including 255 glycoside hydrolases, 76 carbohydrate esterases, 17 polysaccharide lyases, and 113 carbohydrate-binding modules (CBMs).
28161851	5	14	theme	sequence	576:583	arg1	Analysis					553:560	Analysis	553:560	Analysis of nucleotide sequence of predicted gene using a Carbohydrate-Active Enzymes (CAZymes) Analysis Toolkit	553:664	Analysis of nucleotide sequence of predicted gene using a Carbohydrate-Active Enzymes (CAZymes) Analysis Toolkit indicates that strain DD-13 encodes a large set of CAZymes including 255 glycoside hydrolases, 76 carbohydrate esterases, 17 polysaccharide lyases, and 113 carbohydrate-binding modules (CBMs).
28161851	8	15	theme	pure	1274:1277	arg1	alginate					1314:1321	alginate	1314:1321	alginate	1314:1321	The cell growth of strain DD-13 was validated using pure polysaccharides such as agarose or alginate as carbon source as well as by using red and brown seaweed powder as substrate.
28161851	8	15	theme	pure	1274:1277	arg1	polysaccharides					1279:1293	pure polysaccharides	1274:1293	pure polysaccharides such as agarose or alginate	1274:1321	The cell growth of strain DD-13 was validated using pure polysaccharides such as agarose or alginate as carbon source as well as by using red and brown seaweed powder as substrate.
28161851	8	15	theme	pure	1274:1277	arg1	agarose					1303:1309	agarose	1303:1309	agarose	1303:1309	The cell growth of strain DD-13 was validated using pure polysaccharides such as agarose or alginate as carbon source as well as by using red and brown seaweed powder as substrate.
28161851	7	16	with	appended	1112:1119	arg1	CBM					1139:1141	one CBM	1135:1141	one CBM indicating the versatility of strain DD-13 to degrade complex polysaccharides	1135:1219	Some of polysaccharide-degrading genes were highly modular and were appended at least with one CBM indicating the versatility of strain DD-13 to degrade complex polysaccharides.
28161851	5	17	theme	carbohydrate-binding	822:841	arg1	modules					843:849	113 carbohydrate-binding modules	818:849	113 carbohydrate-binding modules (CBMs)	818:856	Analysis of nucleotide sequence of predicted gene using a Carbohydrate-Active Enzymes (CAZymes) Analysis Toolkit indicates that strain DD-13 encodes a large set of CAZymes including 255 glycoside hydrolases, 76 carbohydrate esterases, 17 polysaccharide lyases, and 113 carbohydrate-binding modules (CBMs).
28161851	5	17	theme	carbohydrate-binding	822:841	arg1	CBMs					852:855	CBMs	852:855	CBMs	852:855	Analysis of nucleotide sequence of predicted gene using a Carbohydrate-Active Enzymes (CAZymes) Analysis Toolkit indicates that strain DD-13 encodes a large set of CAZymes including 255 glycoside hydrolases, 76 carbohydrate esterases, 17 polysaccharide lyases, and 113 carbohydrate-binding modules (CBMs).
28161851	6	18	theme	Many	859:862	arg1	carbohydrases					906:918	carbohydrases	906:918	carbohydrases specific for degradation of agar, alginate, carrageenan, chitin, xylan, pullulan, cellulose, starch, β-glucan, pectin, etc	906:1041	Many genes from strain DD-13 were annotated as carbohydrases specific for degradation of agar, alginate, carrageenan, chitin, xylan, pullulan, cellulose, starch, β-glucan, pectin, etc.
28161851	6	18	theme	Many	859:862	arg1	genes					864:868	Many genes	859:868	Many genes from strain DD-13	859:886	Many genes from strain DD-13 were annotated as carbohydrases specific for degradation of agar, alginate, carrageenan, chitin, xylan, pullulan, cellulose, starch, β-glucan, pectin, etc.
28161851	5	19	theme	predicted	588:596	arg1	gene					598:601	predicted gene	588:601	predicted gene	588:601	Analysis of nucleotide sequence of predicted gene using a Carbohydrate-Active Enzymes (CAZymes) Analysis Toolkit indicates that strain DD-13 encodes a large set of CAZymes including 255 glycoside hydrolases, 76 carbohydrate esterases, 17 polysaccharide lyases, and 113 carbohydrate-binding modules (CBMs).
28161851	2	20	theme	G+C	284:286	arg1	content					288:294	G+C content	284:294	G+C content of 57.15%	284:304	The draft genome sequence of strain DD-13 comprised 4,528,106 bp with G+C content of 57.15%.
28161851	3	21	theme	similarity	415:424	arg1	basis					406:410	the basis	402:410	the basis of similarity with the cluster of orthologous groups	402:463	Out of 3479 open reading frames, functions for 3488 protein coding sequences were predicted on the basis of similarity with the cluster of orthologous groups.
28161851	5	22	theme	gene	598:601	arg1	sequence					576:583	nucleotide sequence	565:583	nucleotide sequence of predicted gene	565:601	Analysis of nucleotide sequence of predicted gene using a Carbohydrate-Active Enzymes (CAZymes) Analysis Toolkit indicates that strain DD-13 encodes a large set of CAZymes including 255 glycoside hydrolases, 76 carbohydrate esterases, 17 polysaccharide lyases, and 113 carbohydrate-binding modules (CBMs).
28161851	3	23	theme	coding	367:372	arg1	sequences					374:382	3488 protein coding sequences	354:382	3488 protein coding sequences	354:382	Out of 3479 open reading frames, functions for 3488 protein coding sequences were predicted on the basis of similarity with the cluster of orthologous groups.
28161851	1	24	attach	isolated	171:178	arg2	species					163:169	a novel-type species	150:169	a novel-type species isolated from the mangroves of Goa, India	150:211	Microbulbifer mangrovi strain DD-13T is a novel-type species isolated from the mangroves of Goa, India.
28161851	1	24	attach	isolated	171:178	arg1	mangroves					189:197	the mangroves	185:197	the mangroves of Goa, India	185:211	Microbulbifer mangrovi strain DD-13T is a novel-type species isolated from the mangroves of Goa, India.
28161851	1	24	attach	isolated	171:178	arg2	DD-13T					140:145	Microbulbifer mangrovi strain DD-13T	110:145	Microbulbifer mangrovi strain DD-13T	110:145	Microbulbifer mangrovi strain DD-13T is a novel-type species isolated from the mangroves of Goa, India.
28161851	5	25	theme	CAZymes	717:723	arg1	lyases					806:811	17 polysaccharide lyases	788:811	17 polysaccharide lyases	788:811	Analysis of nucleotide sequence of predicted gene using a Carbohydrate-Active Enzymes (CAZymes) Analysis Toolkit indicates that strain DD-13 encodes a large set of CAZymes including 255 glycoside hydrolases, 76 carbohydrate esterases, 17 polysaccharide lyases, and 113 carbohydrate-binding modules (CBMs).
28161851	5	25	theme	CAZymes	717:723	arg1	DD-13					688:692	strain DD-13	681:692	strain DD-13	681:692	Analysis of nucleotide sequence of predicted gene using a Carbohydrate-Active Enzymes (CAZymes) Analysis Toolkit indicates that strain DD-13 encodes a large set of CAZymes including 255 glycoside hydrolases, 76 carbohydrate esterases, 17 polysaccharide lyases, and 113 carbohydrate-binding modules (CBMs).
28161851	5	25	theme	CAZymes	717:723	arg1	CAZymes					717:723	CAZymes	717:723	CAZymes including 255 glycoside hydrolases, 76 carbohydrate esterases, 17 polysaccharide lyases, and 113 carbohydrate-binding modules (CBMs)	717:856	Analysis of nucleotide sequence of predicted gene using a Carbohydrate-Active Enzymes (CAZymes) Analysis Toolkit indicates that strain DD-13 encodes a large set of CAZymes including 255 glycoside hydrolases, 76 carbohydrate esterases, 17 polysaccharide lyases, and 113 carbohydrate-binding modules (CBMs).
28161851	5	25	theme	CAZymes	717:723	arg1	esterases					777:785	76 carbohydrate esterases	761:785	76 carbohydrate esterases	761:785	Analysis of nucleotide sequence of predicted gene using a Carbohydrate-Active Enzymes (CAZymes) Analysis Toolkit indicates that strain DD-13 encodes a large set of CAZymes including 255 glycoside hydrolases, 76 carbohydrate esterases, 17 polysaccharide lyases, and 113 carbohydrate-binding modules (CBMs).
28161851	5	25	theme	CAZymes	717:723	arg1	set					710:712	a large set	702:712	a large set of CAZymes including 255 glycoside hydrolases, 76 carbohydrate esterases, 17 polysaccharide lyases, and 113 carbohydrate-binding modules (CBMs)	702:856	Analysis of nucleotide sequence of predicted gene using a Carbohydrate-Active Enzymes (CAZymes) Analysis Toolkit indicates that strain DD-13 encodes a large set of CAZymes including 255 glycoside hydrolases, 76 carbohydrate esterases, 17 polysaccharide lyases, and 113 carbohydrate-binding modules (CBMs).
28161851	5	25	theme	CAZymes	717:723	arg1	modules					843:849	113 carbohydrate-binding modules	818:849	113 carbohydrate-binding modules (CBMs)	818:856	Analysis of nucleotide sequence of predicted gene using a Carbohydrate-Active Enzymes (CAZymes) Analysis Toolkit indicates that strain DD-13 encodes a large set of CAZymes including 255 glycoside hydrolases, 76 carbohydrate esterases, 17 polysaccharide lyases, and 113 carbohydrate-binding modules (CBMs).
28161851	5	25	theme	CAZymes	717:723	arg1	hydrolases					749:758	255 glycoside hydrolases	735:758	255 glycoside hydrolases	735:758	Analysis of nucleotide sequence of predicted gene using a Carbohydrate-Active Enzymes (CAZymes) Analysis Toolkit indicates that strain DD-13 encodes a large set of CAZymes including 255 glycoside hydrolases, 76 carbohydrate esterases, 17 polysaccharide lyases, and 113 carbohydrate-binding modules (CBMs).
28161851	0	26	theme	Genome	0:5	arg1	Sequence					7:14	Genome Sequence	0:14	Genome Sequence of Microbulbifer mangrovi DD-13T	0:47	Genome Sequence of Microbulbifer mangrovi DD-13T Reveals Its Versatility to Degrade Multiple Polysaccharides.
28161851	6	27	theme	agar	948:951	arg1	degradation					933:943	degradation	933:943	degradation of agar, alginate, carrageenan, chitin, xylan, pullulan, cellulose, starch, β-glucan, pectin, etc	933:1041	Many genes from strain DD-13 were annotated as carbohydrases specific for degradation of agar, alginate, carrageenan, chitin, xylan, pullulan, cellulose, starch, β-glucan, pectin, etc.
28161851	1	28	theme	Goa	202:204	arg1	mangroves					189:197	the mangroves	185:197	the mangroves of Goa, India	185:211	Microbulbifer mangrovi strain DD-13T is a novel-type species isolated from the mangroves of Goa, India.
28161851	0	29	theme	Microbulbifer	19:31	arg1	DD-13T					42:47	Microbulbifer mangrovi DD-13T	19:47	Microbulbifer mangrovi DD-13T	19:47	Genome Sequence of Microbulbifer mangrovi DD-13T Reveals Its Versatility to Degrade Multiple Polysaccharides.
28161851	8	30	theme	strain	1241:1246	arg1	growth					1231:1236	The cell growth	1222:1236	The cell growth of strain DD-13	1222:1252	The cell growth of strain DD-13 was validated using pure polysaccharides such as agarose or alginate as carbon source as well as by using red and brown seaweed powder as substrate.
28161851	2	31	theme	%	304:304	arg1	content					288:294	G+C content	284:294	G+C content of 57.15%	284:304	The draft genome sequence of strain DD-13 comprised 4,528,106 bp with G+C content of 57.15%.
28161851	3	32	theme	protein	359:365	arg1	sequences					374:382	3488 protein coding sequences	354:382	3488 protein coding sequences	354:382	Out of 3479 open reading frames, functions for 3488 protein coding sequences were predicted on the basis of similarity with the cluster of orthologous groups.
28161851	5	33	theme	strain	681:686	arg1	set					710:712	a large set	702:712	a large set of CAZymes including 255 glycoside hydrolases, 76 carbohydrate esterases, 17 polysaccharide lyases, and 113 carbohydrate-binding modules (CBMs)	702:856	Analysis of nucleotide sequence of predicted gene using a Carbohydrate-Active Enzymes (CAZymes) Analysis Toolkit indicates that strain DD-13 encodes a large set of CAZymes including 255 glycoside hydrolases, 76 carbohydrate esterases, 17 polysaccharide lyases, and 113 carbohydrate-binding modules (CBMs).
28161851	5	33	theme	strain	681:686	arg1	DD-13					688:692	strain DD-13	681:692	strain DD-13	681:692	Analysis of nucleotide sequence of predicted gene using a Carbohydrate-Active Enzymes (CAZymes) Analysis Toolkit indicates that strain DD-13 encodes a large set of CAZymes including 255 glycoside hydrolases, 76 carbohydrate esterases, 17 polysaccharide lyases, and 113 carbohydrate-binding modules (CBMs).
28161851	5	33	theme	strain	681:686	arg1	CAZymes					717:723	CAZymes	717:723	CAZymes including 255 glycoside hydrolases, 76 carbohydrate esterases, 17 polysaccharide lyases, and 113 carbohydrate-binding modules (CBMs)	717:856	Analysis of nucleotide sequence of predicted gene using a Carbohydrate-Active Enzymes (CAZymes) Analysis Toolkit indicates that strain DD-13 encodes a large set of CAZymes including 255 glycoside hydrolases, 76 carbohydrate esterases, 17 polysaccharide lyases, and 113 carbohydrate-binding modules (CBMs).
28161851	8	34	theme	brown	1368:1372	arg1	powder					1382:1387	red and brown seaweed powder	1360:1387	red and brown seaweed powder	1360:1387	The cell growth of strain DD-13 was validated using pure polysaccharides such as agarose or alginate as carbon source as well as by using red and brown seaweed powder as substrate.
28161851	1	35	theme	mangrovi	124:131	arg1	species					163:169	a novel-type species	150:169	a novel-type species isolated from the mangroves of Goa, India	150:211	Microbulbifer mangrovi strain DD-13T is a novel-type species isolated from the mangroves of Goa, India.
28161851	1	35	theme	mangrovi	124:131	arg1	DD-13T					140:145	Microbulbifer mangrovi strain DD-13T	110:145	Microbulbifer mangrovi strain DD-13T	110:145	Microbulbifer mangrovi strain DD-13T is a novel-type species isolated from the mangroves of Goa, India.
28161851	3	36	theme	orthologous	446:456	arg1	groups					458:463	orthologous groups	446:463	orthologous groups	446:463	Out of 3479 open reading frames, functions for 3488 protein coding sequences were predicted on the basis of similarity with the cluster of orthologous groups.
28161851	0	37	theme	DD-13T	42:47	arg1	Sequence					7:14	Genome Sequence	0:14	Genome Sequence of Microbulbifer mangrovi DD-13T	0:47	Genome Sequence of Microbulbifer mangrovi DD-13T Reveals Its Versatility to Degrade Multiple Polysaccharides.
28161851	4	38	theme	rRNA	527:530	arg1	genes					532:536	3 rRNA genes	525:536	3 rRNA genes	525:536	In addition to protein coding sequences, 34 tRNA genes and 3 rRNA genes were detected.
28161851	6	39	from	strain	875:880	arg1	carbohydrases					906:918	carbohydrases	906:918	carbohydrases specific for degradation of agar, alginate, carrageenan, chitin, xylan, pullulan, cellulose, starch, β-glucan, pectin, etc	906:1041	Many genes from strain DD-13 were annotated as carbohydrases specific for degradation of agar, alginate, carrageenan, chitin, xylan, pullulan, cellulose, starch, β-glucan, pectin, etc.
28161851	6	39	from	strain	875:880	arg1	genes					864:868	Many genes	859:868	Many genes from strain DD-13	859:886	Many genes from strain DD-13 were annotated as carbohydrases specific for degradation of agar, alginate, carrageenan, chitin, xylan, pullulan, cellulose, starch, β-glucan, pectin, etc.
28161851	9	40	theme	metabolizable	1526:1538	arg1	sugars					1549:1554	metabolizable reducing sugars	1526:1554	metabolizable reducing sugars	1526:1554	The homologous carbohydrase produced by strain DD-13 during growth degraded the polysaccharide, ensuring the production of metabolizable reducing sugars.
28161851	0	41	theme	mangrovi	33:40	arg1	DD-13T					42:47	Microbulbifer mangrovi DD-13T	19:47	Microbulbifer mangrovi DD-13T	19:47	Genome Sequence of Microbulbifer mangrovi DD-13T Reveals Its Versatility to Degrade Multiple Polysaccharides.
28161851	6	42	theme	specific	920:927	arg1	carbohydrases					906:918	carbohydrases	906:918	carbohydrases specific for degradation of agar, alginate, carrageenan, chitin, xylan, pullulan, cellulose, starch, β-glucan, pectin, etc	906:1041	Many genes from strain DD-13 were annotated as carbohydrases specific for degradation of agar, alginate, carrageenan, chitin, xylan, pullulan, cellulose, starch, β-glucan, pectin, etc.
28161851	6	42	theme	specific	920:927	arg1	genes					864:868	Many genes	859:868	Many genes from strain DD-13	859:886	Many genes from strain DD-13 were annotated as carbohydrases specific for degradation of agar, alginate, carrageenan, chitin, xylan, pullulan, cellulose, starch, β-glucan, pectin, etc.
28161851	10	43	theme	several	1571:1577	arg1	polysaccharides					1585:1599	several other polysaccharides	1571:1599	several other polysaccharides such as carrageenan, xylan, pullulan, pectin, starch, and carboxymethyl cellulose	1571:1681	Additionally, several other polysaccharides such as carrageenan, xylan, pullulan, pectin, starch, and carboxymethyl cellulose were also corroborated as growth substrate for strain DD-13 and were associated with concomitant production of homologous carbohydrase.
28161851	10	43	theme	several	1571:1577	arg1	cellulose					1673:1681	carboxymethyl cellulose	1659:1681	carboxymethyl cellulose	1659:1681	Additionally, several other polysaccharides such as carrageenan, xylan, pullulan, pectin, starch, and carboxymethyl cellulose were also corroborated as growth substrate for strain DD-13 and were associated with concomitant production of homologous carbohydrase.
28161851	10	43	theme	several	1571:1577	arg1	substrate					1716:1724	growth substrate	1709:1724	growth substrate for strain DD-13	1709:1741	Additionally, several other polysaccharides such as carrageenan, xylan, pullulan, pectin, starch, and carboxymethyl cellulose were also corroborated as growth substrate for strain DD-13 and were associated with concomitant production of homologous carbohydrase.
28161851	10	43	theme	several	1571:1577	arg1	carrageenan					1609:1619	carrageenan	1609:1619	carrageenan	1609:1619	Additionally, several other polysaccharides such as carrageenan, xylan, pullulan, pectin, starch, and carboxymethyl cellulose were also corroborated as growth substrate for strain DD-13 and were associated with concomitant production of homologous carbohydrase.
28161851	10	43	theme	several	1571:1577	arg1	pectin					1639:1644	pectin	1639:1644	pectin	1639:1644	Additionally, several other polysaccharides such as carrageenan, xylan, pullulan, pectin, starch, and carboxymethyl cellulose were also corroborated as growth substrate for strain DD-13 and were associated with concomitant production of homologous carbohydrase.
28161851	10	43	theme	several	1571:1577	arg1	starch					1647:1652	starch	1647:1652	starch	1647:1652	Additionally, several other polysaccharides such as carrageenan, xylan, pullulan, pectin, starch, and carboxymethyl cellulose were also corroborated as growth substrate for strain DD-13 and were associated with concomitant production of homologous carbohydrase.
28161851	10	43	theme	several	1571:1577	arg1	xylan					1622:1626	xylan	1622:1626	xylan	1622:1626	Additionally, several other polysaccharides such as carrageenan, xylan, pullulan, pectin, starch, and carboxymethyl cellulose were also corroborated as growth substrate for strain DD-13 and were associated with concomitant production of homologous carbohydrase.
28161851	10	43	theme	several	1571:1577	arg1	pullulan					1629:1636	pullulan	1629:1636	pullulan	1629:1636	Additionally, several other polysaccharides such as carrageenan, xylan, pullulan, pectin, starch, and carboxymethyl cellulose were also corroborated as growth substrate for strain DD-13 and were associated with concomitant production of homologous carbohydrase.
28161851	10	44	theme	carbohydrase	1805:1816	arg1	production					1780:1789	concomitant production	1768:1789	concomitant production of homologous carbohydrase	1768:1816	Additionally, several other polysaccharides such as carrageenan, xylan, pullulan, pectin, starch, and carboxymethyl cellulose were also corroborated as growth substrate for strain DD-13 and were associated with concomitant production of homologous carbohydrase.
28161851	8	45	theme	carbon	1326:1331	arg1	source					1333:1338	carbon source	1326:1338	carbon source	1326:1338	The cell growth of strain DD-13 was validated using pure polysaccharides such as agarose or alginate as carbon source as well as by using red and brown seaweed powder as substrate.
28161851	7	46	theme	polysaccharide-degrading	1052:1075	arg1	genes					1077:1081	polysaccharide-degrading genes	1052:1081	polysaccharide-degrading genes	1052:1081	Some of polysaccharide-degrading genes were highly modular and were appended at least with one CBM indicating the versatility of strain DD-13 to degrade complex polysaccharides.
28161851	8	47	theme	seaweed	1374:1380	arg1	powder					1382:1387	red and brown seaweed powder	1360:1387	red and brown seaweed powder	1360:1387	The cell growth of strain DD-13 was validated using pure polysaccharides such as agarose or alginate as carbon source as well as by using red and brown seaweed powder as substrate.
28161851	2	48	theme	genome	224:229	arg1	sequence					231:238	The draft genome sequence	214:238	The draft genome sequence of strain DD-13	214:254	The draft genome sequence of strain DD-13 comprised 4,528,106 bp with G+C content of 57.15%.
28161851	1	49	theme	strain	133:138	arg1	species					163:169	a novel-type species	150:169	a novel-type species isolated from the mangroves of Goa, India	150:211	Microbulbifer mangrovi strain DD-13T is a novel-type species isolated from the mangroves of Goa, India.
28161851	1	49	theme	strain	133:138	arg1	DD-13T					140:145	Microbulbifer mangrovi strain DD-13T	110:145	Microbulbifer mangrovi strain DD-13T	110:145	Microbulbifer mangrovi strain DD-13T is a novel-type species isolated from the mangroves of Goa, India.
28161851	5	50	theme	carbohydrate	764:775	arg1	esterases					777:785	76 carbohydrate esterases	761:785	76 carbohydrate esterases	761:785	Analysis of nucleotide sequence of predicted gene using a Carbohydrate-Active Enzymes (CAZymes) Analysis Toolkit indicates that strain DD-13 encodes a large set of CAZymes including 255 glycoside hydrolases, 76 carbohydrate esterases, 17 polysaccharide lyases, and 113 carbohydrate-binding modules (CBMs).
28161851	5	51	theme	Analysis	649:656	arg1	Toolkit					658:664	a Carbohydrate-Active Enzymes (CAZymes) Analysis Toolkit	609:664	a Carbohydrate-Active Enzymes (CAZymes) Analysis Toolkit	609:664	Analysis of nucleotide sequence of predicted gene using a Carbohydrate-Active Enzymes (CAZymes) Analysis Toolkit indicates that strain DD-13 encodes a large set of CAZymes including 255 glycoside hydrolases, 76 carbohydrate esterases, 17 polysaccharide lyases, and 113 carbohydrate-binding modules (CBMs).
28161851	2	52	theme	draft	218:222	arg1	sequence					231:238	The draft genome sequence	214:238	The draft genome sequence of strain DD-13	214:254	The draft genome sequence of strain DD-13 comprised 4,528,106 bp with G+C content of 57.15%.
28161851	8	53	theme	cell	1226:1229	arg1	growth					1231:1236	The cell growth	1222:1236	The cell growth of strain DD-13	1222:1252	The cell growth of strain DD-13 was validated using pure polysaccharides such as agarose or alginate as carbon source as well as by using red and brown seaweed powder as substrate.
28161851	10	54	theme	other	1579:1583	arg1	polysaccharides					1585:1599	several other polysaccharides	1571:1599	several other polysaccharides such as carrageenan, xylan, pullulan, pectin, starch, and carboxymethyl cellulose	1571:1681	Additionally, several other polysaccharides such as carrageenan, xylan, pullulan, pectin, starch, and carboxymethyl cellulose were also corroborated as growth substrate for strain DD-13 and were associated with concomitant production of homologous carbohydrase.
28161851	10	54	theme	other	1579:1583	arg1	cellulose					1673:1681	carboxymethyl cellulose	1659:1681	carboxymethyl cellulose	1659:1681	Additionally, several other polysaccharides such as carrageenan, xylan, pullulan, pectin, starch, and carboxymethyl cellulose were also corroborated as growth substrate for strain DD-13 and were associated with concomitant production of homologous carbohydrase.
28161851	10	54	theme	other	1579:1583	arg1	substrate					1716:1724	growth substrate	1709:1724	growth substrate for strain DD-13	1709:1741	Additionally, several other polysaccharides such as carrageenan, xylan, pullulan, pectin, starch, and carboxymethyl cellulose were also corroborated as growth substrate for strain DD-13 and were associated with concomitant production of homologous carbohydrase.
28161851	10	54	theme	other	1579:1583	arg1	carrageenan					1609:1619	carrageenan	1609:1619	carrageenan	1609:1619	Additionally, several other polysaccharides such as carrageenan, xylan, pullulan, pectin, starch, and carboxymethyl cellulose were also corroborated as growth substrate for strain DD-13 and were associated with concomitant production of homologous carbohydrase.
28161851	10	54	theme	other	1579:1583	arg1	pectin					1639:1644	pectin	1639:1644	pectin	1639:1644	Additionally, several other polysaccharides such as carrageenan, xylan, pullulan, pectin, starch, and carboxymethyl cellulose were also corroborated as growth substrate for strain DD-13 and were associated with concomitant production of homologous carbohydrase.
28161851	10	54	theme	other	1579:1583	arg1	starch					1647:1652	starch	1647:1652	starch	1647:1652	Additionally, several other polysaccharides such as carrageenan, xylan, pullulan, pectin, starch, and carboxymethyl cellulose were also corroborated as growth substrate for strain DD-13 and were associated with concomitant production of homologous carbohydrase.
28161851	10	54	theme	other	1579:1583	arg1	xylan					1622:1626	xylan	1622:1626	xylan	1622:1626	Additionally, several other polysaccharides such as carrageenan, xylan, pullulan, pectin, starch, and carboxymethyl cellulose were also corroborated as growth substrate for strain DD-13 and were associated with concomitant production of homologous carbohydrase.
28161851	10	54	theme	other	1579:1583	arg1	pullulan					1629:1636	pullulan	1629:1636	pullulan	1629:1636	Additionally, several other polysaccharides such as carrageenan, xylan, pullulan, pectin, starch, and carboxymethyl cellulose were also corroborated as growth substrate for strain DD-13 and were associated with concomitant production of homologous carbohydrase.
28161851	4	55	theme	tRNA	510:513	arg1	genes					515:519	34 tRNA genes	507:519	34 tRNA genes	507:519	In addition to protein coding sequences, 34 tRNA genes and 3 rRNA genes were detected.
28161851	3	56	with	similarity	415:424	arg1	cluster					435:441	the cluster	431:441	the cluster of orthologous groups	431:463	Out of 3479 open reading frames, functions for 3488 protein coding sequences were predicted on the basis of similarity with the cluster of orthologous groups.
28161851	9	57	theme	reducing	1540:1547	arg1	sugars					1549:1554	metabolizable reducing sugars	1526:1554	metabolizable reducing sugars	1526:1554	The homologous carbohydrase produced by strain DD-13 during growth degraded the polysaccharide, ensuring the production of metabolizable reducing sugars.
28161851	3	58	theme	groups	458:463	arg1	cluster					435:441	the cluster	431:441	the cluster of orthologous groups	431:463	Out of 3479 open reading frames, functions for 3488 protein coding sequences were predicted on the basis of similarity with the cluster of orthologous groups.
28161851	1	59	theme	Microbulbifer	110:122	arg1	species					163:169	a novel-type species	150:169	a novel-type species isolated from the mangroves of Goa, India	150:211	Microbulbifer mangrovi strain DD-13T is a novel-type species isolated from the mangroves of Goa, India.
28161851	1	59	theme	Microbulbifer	110:122	arg1	DD-13T					140:145	Microbulbifer mangrovi strain DD-13T	110:145	Microbulbifer mangrovi strain DD-13T	110:145	Microbulbifer mangrovi strain DD-13T is a novel-type species isolated from the mangroves of Goa, India.
28161851	5	60	theme	polysaccharide	791:804	arg1	lyases					806:811	17 polysaccharide lyases	788:811	17 polysaccharide lyases	788:811	Analysis of nucleotide sequence of predicted gene using a Carbohydrate-Active Enzymes (CAZymes) Analysis Toolkit indicates that strain DD-13 encodes a large set of CAZymes including 255 glycoside hydrolases, 76 carbohydrate esterases, 17 polysaccharide lyases, and 113 carbohydrate-binding modules (CBMs).
28161851	7	61	theme	complex	1197:1203	arg1	polysaccharides					1205:1219	complex polysaccharides	1197:1219	complex polysaccharides	1197:1219	Some of polysaccharide-degrading genes were highly modular and were appended at least with one CBM indicating the versatility of strain DD-13 to degrade complex polysaccharides.
28161851	3	62	theme	open	319:322	arg1	frames					332:337	3479 open reading frames	314:337	3479 open reading frames	314:337	Out of 3479 open reading frames, functions for 3488 protein coding sequences were predicted on the basis of similarity with the cluster of orthologous groups.
28161851	8	63	theme	red	1360:1362	arg1	powder					1382:1387	red and brown seaweed powder	1360:1387	red and brown seaweed powder	1360:1387	The cell growth of strain DD-13 was validated using pure polysaccharides such as agarose or alginate as carbon source as well as by using red and brown seaweed powder as substrate.
28161851	1	64	theme	novel-type	152:161	arg1	species					163:169	a novel-type species	150:169	a novel-type species isolated from the mangroves of Goa, India	150:211	Microbulbifer mangrovi strain DD-13T is a novel-type species isolated from the mangroves of Goa, India.
28161851	1	64	theme	novel-type	152:161	arg1	DD-13T					140:145	Microbulbifer mangrovi strain DD-13T	110:145	Microbulbifer mangrovi strain DD-13T	110:145	Microbulbifer mangrovi strain DD-13T is a novel-type species isolated from the mangroves of Goa, India.
28161851	3	65	theme	reading	324:330	arg1	frames					332:337	3479 open reading frames	314:337	3479 open reading frames	314:337	Out of 3479 open reading frames, functions for 3488 protein coding sequences were predicted on the basis of similarity with the cluster of orthologous groups.
28161851	4	66	theme	protein	481:487	arg1	sequences					496:504	protein coding sequences	481:504	protein coding sequences	481:504	In addition to protein coding sequences, 34 tRNA genes and 3 rRNA genes were detected.
28161851	2	67	theme	strain	243:248	arg1	sequence					231:238	The draft genome sequence	214:238	The draft genome sequence of strain DD-13	214:254	The draft genome sequence of strain DD-13 comprised 4,528,106 bp with G+C content of 57.15%.
28161851	10	68	theme	carboxymethyl	1659:1671	arg1	cellulose					1673:1681	carboxymethyl cellulose	1659:1681	carboxymethyl cellulose	1659:1681	Additionally, several other polysaccharides such as carrageenan, xylan, pullulan, pectin, starch, and carboxymethyl cellulose were also corroborated as growth substrate for strain DD-13 and were associated with concomitant production of homologous carbohydrase.
26167280	8	0	theme	animals	1476:1482	arg1	metagenome					1453:1462	the microbial metagenome	1439:1462	the microbial metagenome of milk-fed animals	1439:1482	Predicted enzymes active on milk-derived glycans that are otherwise indigestible to the host animal were enriched in the microbial metagenome of milk-fed animals.
26167280	1	1	dep	BACKGROUND	67:76	arg1	colonized					108:116	colonized	108:116	is rapidly colonized by a complex microbial community, whose importance for host health is becoming increasingly clear	97:214	BACKGROUND The newborn mammal is rapidly colonized by a complex microbial community, whose importance for host health is becoming increasingly clear.
26167280	7	2	used	used	1171:1174	arg2	sequencing					1156:1165	Metagenomic sequencing	1144:1165	Metagenomic sequencing	1144:1165	Metagenomic sequencing was used to characterize a subset of animals before and after weaning, which identified glycan degradation pathways differing significantly between diets.
26167280	6	3	theme	Microbial	1030:1038	arg1	communities					1040:1050	Microbial communities	1030:1050	Microbial communities	1030:1050	Microbial communities were clearly distinguishable based on diet, being relatively stable absent dietary changes.
26167280	5	4	theme	exclusive	938:946	arg1	diet					948:951	an exclusive diet	935:951	an exclusive diet of sow milk to a starter diet composed of plant and animal-based components	935:1027	RESULTS We characterized the fecal microbiome of pigs from birth through 7 weeks of age, during which the animals were transitioned from an exclusive diet of sow milk to a starter diet composed of plant and animal-based components.
26167280	3	5	from	similarity	482:491	arg1	studies					555:561	experimental studies	542:561	experimental studies	542:561	Pigs present an attractive model for nursing humans, given physiological and compositional similarity of pig and human milk and the utility of pigs in experimental studies.
26167280	3	6	theme	milk	510:513	arg1	similarity					482:491	physiological and compositional similarity	450:491	physiological and compositional similarity of pig and human milk	450:513	Pigs present an attractive model for nursing humans, given physiological and compositional similarity of pig and human milk and the utility of pigs in experimental studies.
26167280	3	6	theme	milk	510:513	arg1	utility					523:529	the utility	519:529	the utility of pigs in experimental studies	519:561	Pigs present an attractive model for nursing humans, given physiological and compositional similarity of pig and human milk and the utility of pigs in experimental studies.
26167280	9	7	theme	animals	1533:1539	arg1	metagenome					1512:1521	the bacterial metagenome	1498:1521	the bacterial metagenome of weaned animals	1498:1539	In contrast, the bacterial metagenome of weaned animals was enriched in functional pathways involved in plant glycan deconstruction and consumption.
26167280	4	8	theme	molecular	685:693	arg1	methods					695:701	modern molecular methods	678:701	modern molecular methods	678:701	However, there is a paucity of data examining the gut microbiome in nursing pigs from birth through weaning using modern molecular methods and fewer experimental studies that examine the impact of diet on these microbial communities.
26167280	7	9	theme	glycan	1255:1260	arg1	pathways					1274:1281	glycan degradation pathways	1255:1281	glycan degradation pathways differing significantly between diets	1255:1319	Metagenomic sequencing was used to characterize a subset of animals before and after weaning, which identified glycan degradation pathways differing significantly between diets.
26167280	4	10	theme	fewer	707:711	arg1	studies					726:732	fewer experimental studies	707:732	fewer experimental studies	707:732	However, there is a paucity of data examining the gut microbiome in nursing pigs from birth through weaning using modern molecular methods and fewer experimental studies that examine the impact of diet on these microbial communities.
26167280	3	11	theme	compositional	468:480	arg1	similarity					482:491	physiological and compositional similarity	450:491	physiological and compositional similarity of pig and human milk	450:513	Pigs present an attractive model for nursing humans, given physiological and compositional similarity of pig and human milk and the utility of pigs in experimental studies.
26167280	4	12	from	microbiome	618:627	arg1	pigs					640:643	nursing pigs	632:643	nursing pigs	632:643	However, there is a paucity of data examining the gut microbiome in nursing pigs from birth through weaning using modern molecular methods and fewer experimental studies that examine the impact of diet on these microbial communities.
26167280	5	13	theme	age	882:884	arg1	7 weeks					871:877	7 weeks	871:877	7 weeks	871:877	RESULTS We characterized the fecal microbiome of pigs from birth through 7 weeks of age, during which the animals were transitioned from an exclusive diet of sow milk to a starter diet composed of plant and animal-based components.
26167280	2	14	theme	early	257:261	arg1	community					263:271	the early community	253:271	the early community	253:271	Understanding the forces that shape the early community, especially during the nursing period, is critical to gain insight into how this consortium of microbes is assembled.
26167280	10	15	theme	gut	1650:1652	arg1	microbiome					1654:1663	The gut microbiome	1646:1663	The gut microbiome in young pigs	1646:1677	CONCLUSIONS The gut microbiome in young pigs is dramatically shaped by the composition of dietary glycans, reflected by the different functional capacities of the microbiome before and after weaning.
26167280	7	16	theme	Metagenomic	1144:1154	arg1	sequencing					1156:1165	Metagenomic sequencing	1144:1165	Metagenomic sequencing	1144:1165	Metagenomic sequencing was used to characterize a subset of animals before and after weaning, which identified glycan degradation pathways differing significantly between diets.
26167280	10	17	theme	young	1668:1672	arg1	pigs					1674:1677	young pigs	1668:1677	young pigs	1668:1677	CONCLUSIONS The gut microbiome in young pigs is dramatically shaped by the composition of dietary glycans, reflected by the different functional capacities of the microbiome before and after weaning.
26167280	8	18	theme	Predicted	1322:1330	arg1	enzymes					1332:1338	Predicted enzymes	1322:1338	Predicted enzymes active on milk-derived glycans that are otherwise indigestible to the host animal	1322:1420	Predicted enzymes active on milk-derived glycans that are otherwise indigestible to the host animal were enriched in the microbial metagenome of milk-fed animals.
26167280	3	19	theme	human	504:508	arg1	milk					510:513	human milk	504:513	human milk	504:513	Pigs present an attractive model for nursing humans, given physiological and compositional similarity of pig and human milk and the utility of pigs in experimental studies.
26167280	3	20	theme	attractive	407:416	arg1	model					418:422	an attractive model	404:422	an attractive model for nursing humans	404:441	Pigs present an attractive model for nursing humans, given physiological and compositional similarity of pig and human milk and the utility of pigs in experimental studies.
26167280	9	21	theme	bacterial	1502:1510	arg1	metagenome					1512:1521	the bacterial metagenome	1498:1521	the bacterial metagenome of weaned animals	1498:1539	In contrast, the bacterial metagenome of weaned animals was enriched in functional pathways involved in plant glycan deconstruction and consumption.
26167280	10	22	theme	glycans	1732:1738	arg1	composition					1709:1719	the composition	1705:1719	the composition	1705:1719	CONCLUSIONS The gut microbiome in young pigs is dramatically shaped by the composition of dietary glycans, reflected by the different functional capacities of the microbiome before and after weaning.
26167280	2	23	theme	nursing	296:302	arg1	period					304:309	the nursing period	292:309	the nursing period	292:309	Understanding the forces that shape the early community, especially during the nursing period, is critical to gain insight into how this consortium of microbes is assembled.
26167280	5	24	theme	fecal	827:831	arg1	microbiome					833:842	the fecal microbiome	823:842	the fecal microbiome of pigs	823:850	RESULTS We characterized the fecal microbiome of pigs from birth through 7 weeks of age, during which the animals were transitioned from an exclusive diet of sow milk to a starter diet composed of plant and animal-based components.
26167280	10	25	theme	microbiome	1797:1806	arg1	capacities					1779:1788	the different functional capacities	1754:1788	the different functional capacities of the microbiome before and after weaning	1754:1831	CONCLUSIONS The gut microbiome in young pigs is dramatically shaped by the composition of dietary glycans, reflected by the different functional capacities of the microbiome before and after weaning.
26167280	9	26	theme	functional	1557:1566	arg1	pathways					1568:1575	functional pathways	1557:1575	functional pathways involved in plant glycan deconstruction and consumption	1557:1631	In contrast, the bacterial metagenome of weaned animals was enriched in functional pathways involved in plant glycan deconstruction and consumption.
26167280	3	27	theme	pigs	534:537	arg1	similarity					482:491	physiological and compositional similarity	450:491	physiological and compositional similarity of pig and human milk	450:513	Pigs present an attractive model for nursing humans, given physiological and compositional similarity of pig and human milk and the utility of pigs in experimental studies.
26167280	3	27	theme	pigs	534:537	arg1	utility					523:529	the utility	519:529	the utility of pigs in experimental studies	519:561	Pigs present an attractive model for nursing humans, given physiological and compositional similarity of pig and human milk and the utility of pigs in experimental studies.
26167280	4	28	theme	gut	614:616	arg1	microbiome					618:627	the gut microbiome	610:627	the gut microbiome in nursing pigs from birth	610:654	However, there is a paucity of data examining the gut microbiome in nursing pigs from birth through weaning using modern molecular methods and fewer experimental studies that examine the impact of diet on these microbial communities.
26167280	0	29	theme	gut	16:18	arg1	microbiome					20:29	the gut microbiome	12:29	the gut microbiome of pigs	12:37	Diet shapes the gut microbiome of pigs during nursing and weaning.
26167280	10	30	theme	dietary	1724:1730	arg1	glycans					1732:1738	dietary glycans	1724:1738	dietary glycans	1724:1738	CONCLUSIONS The gut microbiome in young pigs is dramatically shaped by the composition of dietary glycans, reflected by the different functional capacities of the microbiome before and after weaning.
26167280	9	31	theme	glycan	1595:1600	arg1	deconstruction					1602:1615	plant glycan deconstruction	1589:1615	plant glycan deconstruction	1589:1615	In contrast, the bacterial metagenome of weaned animals was enriched in functional pathways involved in plant glycan deconstruction and consumption.
26167280	2	32	dep	critical	315:322	arg1	Understanding					217:229	Understanding	217:229	Understanding the forces that shape the early community	217:271	Understanding the forces that shape the early community, especially during the nursing period, is critical to gain insight into how this consortium of microbes is assembled.
26167280	3	33	theme	nursing	428:434	arg1	humans					436:441	nursing humans	428:441	nursing humans	428:441	Pigs present an attractive model for nursing humans, given physiological and compositional similarity of pig and human milk and the utility of pigs in experimental studies.
26167280	4	34	theme	modern	678:683	arg1	methods					695:701	modern molecular methods	678:701	modern molecular methods	678:701	However, there is a paucity of data examining the gut microbiome in nursing pigs from birth through weaning using modern molecular methods and fewer experimental studies that examine the impact of diet on these microbial communities.
26167280	6	35	theme	absent	1120:1125	arg1	changes					1135:1141	relatively stable absent dietary changes	1102:1141	relatively stable absent dietary changes	1102:1141	Microbial communities were clearly distinguishable based on diet, being relatively stable absent dietary changes.
26167280	8	36	theme	milk-derived	1350:1361	arg1	glycans					1363:1369	milk-derived glycans	1350:1369	milk-derived glycans that are otherwise indigestible to the host animal	1350:1420	Predicted enzymes active on milk-derived glycans that are otherwise indigestible to the host animal were enriched in the microbial metagenome of milk-fed animals.
26167280	7	37	theme	degradation	1262:1272	arg1	pathways					1274:1281	glycan degradation pathways	1255:1281	glycan degradation pathways differing significantly between diets	1255:1319	Metagenomic sequencing was used to characterize a subset of animals before and after weaning, which identified glycan degradation pathways differing significantly between diets.
26167280	10	38	theme	functional	1768:1777	arg1	capacities					1779:1788	the different functional capacities	1754:1788	the different functional capacities of the microbiome before and after weaning	1754:1831	CONCLUSIONS The gut microbiome in young pigs is dramatically shaped by the composition of dietary glycans, reflected by the different functional capacities of the microbiome before and after weaning.
26167280	7	39	theme	animals	1204:1210	arg1	subset					1194:1199	a subset	1192:1199	a subset	1192:1199	Metagenomic sequencing was used to characterize a subset of animals before and after weaning, which identified glycan degradation pathways differing significantly between diets.
26167280	5	40	theme	sow	956:958	arg1	milk					960:963	sow milk	956:963	sow milk	956:963	RESULTS We characterized the fecal microbiome of pigs from birth through 7 weeks of age, during which the animals were transitioned from an exclusive diet of sow milk to a starter diet composed of plant and animal-based components.
26167280	10	41	dep	CONCLUSIONS	1634:1644	arg1	shaped					1695:1700	shaped	1695:1700	is dramatically shaped by the composition of dietary glycans, reflected by the different functional capacities of the microbiome before and after weaning	1679:1831	CONCLUSIONS The gut microbiome in young pigs is dramatically shaped by the composition of dietary glycans, reflected by the different functional capacities of the microbiome before and after weaning.
26167280	4	42	theme	data	595:598	arg1	paucity					584:590	a paucity	582:590	a paucity of data examining the gut microbiome in nursing pigs from birth through weaning using modern molecular methods and fewer experimental studies that examine the impact of diet on these microbial communities	582:795	However, there is a paucity of data examining the gut microbiome in nursing pigs from birth through weaning using modern molecular methods and fewer experimental studies that examine the impact of diet on these microbial communities.
26167280	5	43	theme	animal-based	1005:1016	arg1	components					1018:1027	animal-based components	1005:1027	animal-based components	1005:1027	RESULTS We characterized the fecal microbiome of pigs from birth through 7 weeks of age, during which the animals were transitioned from an exclusive diet of sow milk to a starter diet composed of plant and animal-based components.
26167280	4	44	theme	diet	761:764	arg1	impact					751:756	the impact	747:756	the impact of diet on these microbial communities	747:795	However, there is a paucity of data examining the gut microbiome in nursing pigs from birth through weaning using modern molecular methods and fewer experimental studies that examine the impact of diet on these microbial communities.
26167280	4	45	from	impact	751:756	arg1	communities					785:795	these microbial communities	769:795	these microbial communities	769:795	However, there is a paucity of data examining the gut microbiome in nursing pigs from birth through weaning using modern molecular methods and fewer experimental studies that examine the impact of diet on these microbial communities.
26167280	2	46	theme	microbes	368:375	arg1	consortium					354:363	this consortium	349:363	this consortium of microbes	349:375	Understanding the forces that shape the early community, especially during the nursing period, is critical to gain insight into how this consortium of microbes is assembled.
26167280	8	47	link	milk-derived	1350:1361	arg1	glycans					1363:1369	milk-derived glycans	1350:1369	milk-derived glycans that are otherwise indigestible to the host animal	1350:1420	Predicted enzymes active on milk-derived glycans that are otherwise indigestible to the host animal were enriched in the microbial metagenome of milk-fed animals.
26167280	4	48	theme	microbial	775:783	arg1	communities					785:795	these microbial communities	769:795	these microbial communities	769:795	However, there is a paucity of data examining the gut microbiome in nursing pigs from birth through weaning using modern molecular methods and fewer experimental studies that examine the impact of diet on these microbial communities.
26167280	6	49	theme	stable	1113:1118	arg1	changes					1135:1141	relatively stable absent dietary changes	1102:1141	relatively stable absent dietary changes	1102:1141	Microbial communities were clearly distinguishable based on diet, being relatively stable absent dietary changes.
26167280	3	50	theme	physiological	450:462	arg1	similarity					482:491	physiological and compositional similarity	450:491	physiological and compositional similarity of pig and human milk	450:513	Pigs present an attractive model for nursing humans, given physiological and compositional similarity of pig and human milk and the utility of pigs in experimental studies.
26167280	0	51	theme	pigs	34:37	arg1	microbiome					20:29	the gut microbiome	12:29	the gut microbiome of pigs	12:37	Diet shapes the gut microbiome of pigs during nursing and weaning.
26167280	6	52	theme	dietary	1127:1133	arg1	changes					1135:1141	relatively stable absent dietary changes	1102:1141	relatively stable absent dietary changes	1102:1141	Microbial communities were clearly distinguishable based on diet, being relatively stable absent dietary changes.
26167280	5	53	theme	starter	970:976	arg1	diet					978:981	a starter diet	968:981	a starter diet composed of plant and animal-based components	968:1027	RESULTS We characterized the fecal microbiome of pigs from birth through 7 weeks of age, during which the animals were transitioned from an exclusive diet of sow milk to a starter diet composed of plant and animal-based components.
26167280	3	54	theme	experimental	542:553	arg1	studies					555:561	experimental studies	542:561	experimental studies	542:561	Pigs present an attractive model for nursing humans, given physiological and compositional similarity of pig and human milk and the utility of pigs in experimental studies.
26167280	10	55	from	microbiome	1654:1663	arg1	pigs					1674:1677	young pigs	1668:1677	young pigs	1668:1677	CONCLUSIONS The gut microbiome in young pigs is dramatically shaped by the composition of dietary glycans, reflected by the different functional capacities of the microbiome before and after weaning.
26167280	10	56	theme	different	1758:1766	arg1	capacities					1779:1788	the different functional capacities	1754:1788	the different functional capacities of the microbiome before and after weaning	1754:1831	CONCLUSIONS The gut microbiome in young pigs is dramatically shaped by the composition of dietary glycans, reflected by the different functional capacities of the microbiome before and after weaning.
26167280	9	57	theme	plant	1589:1593	arg1	deconstruction					1602:1615	plant glycan deconstruction	1589:1615	plant glycan deconstruction	1589:1615	In contrast, the bacterial metagenome of weaned animals was enriched in functional pathways involved in plant glycan deconstruction and consumption.
26167280	8	58	theme	microbial	1443:1451	arg1	metagenome					1453:1462	the microbial metagenome	1439:1462	the microbial metagenome of milk-fed animals	1439:1482	Predicted enzymes active on milk-derived glycans that are otherwise indigestible to the host animal were enriched in the microbial metagenome of milk-fed animals.
26167280	5	59	dep	RESULTS	798:804	arg1	characterized					809:821	characterized	809:821	characterized the fecal microbiome of pigs from birth through 7 weeks of age, during which the animals were transitioned from an exclusive diet of sow milk to a starter diet composed of plant and animal-based components	809:1027	RESULTS We characterized the fecal microbiome of pigs from birth through 7 weeks of age, during which the animals were transitioned from an exclusive diet of sow milk to a starter diet composed of plant and animal-based components.
26167280	5	60	theme	milk	960:963	arg1	diet					948:951	an exclusive diet	935:951	an exclusive diet of sow milk to a starter diet composed of plant and animal-based components	935:1027	RESULTS We characterized the fecal microbiome of pigs from birth through 7 weeks of age, during which the animals were transitioned from an exclusive diet of sow milk to a starter diet composed of plant and animal-based components.
26167280	3	61	from	utility	523:529	arg1	studies					555:561	experimental studies	542:561	experimental studies	542:561	Pigs present an attractive model for nursing humans, given physiological and compositional similarity of pig and human milk and the utility of pigs in experimental studies.
26167280	4	62	theme	studies	726:732	arg1	paucity					584:590	a paucity	582:590	a paucity of data examining the gut microbiome in nursing pigs from birth through weaning using modern molecular methods and fewer experimental studies that examine the impact of diet on these microbial communities	582:795	However, there is a paucity of data examining the gut microbiome in nursing pigs from birth through weaning using modern molecular methods and fewer experimental studies that examine the impact of diet on these microbial communities.
26167280	8	63	theme	milk-fed	1467:1474	arg1	animals					1476:1482	milk-fed animals	1467:1482	milk-fed animals	1467:1482	Predicted enzymes active on milk-derived glycans that are otherwise indigestible to the host animal were enriched in the microbial metagenome of milk-fed animals.
26167280	4	64	theme	experimental	713:724	arg1	studies					726:732	fewer experimental studies	707:732	fewer experimental studies	707:732	However, there is a paucity of data examining the gut microbiome in nursing pigs from birth through weaning using modern molecular methods and fewer experimental studies that examine the impact of diet on these microbial communities.
26167280	1	65	theme	complex	123:129	arg1	community					141:149	a complex microbial community	121:149	a complex microbial community	121:149	BACKGROUND The newborn mammal is rapidly colonized by a complex microbial community, whose importance for host health is becoming increasingly clear.
26167280	8	66	theme	active	1340:1345	arg1	enzymes					1332:1338	Predicted enzymes	1322:1338	Predicted enzymes active on milk-derived glycans that are otherwise indigestible to the host animal	1322:1420	Predicted enzymes active on milk-derived glycans that are otherwise indigestible to the host animal were enriched in the microbial metagenome of milk-fed animals.
26167280	5	67	theme	pigs	847:850	arg1	microbiome					833:842	the fecal microbiome	823:842	the fecal microbiome of pigs	823:850	RESULTS We characterized the fecal microbiome of pigs from birth through 7 weeks of age, during which the animals were transitioned from an exclusive diet of sow milk to a starter diet composed of plant and animal-based components.
26167280	1	68	theme	host	173:176	arg1	health					178:183	host health	173:183	host health	173:183	BACKGROUND The newborn mammal is rapidly colonized by a complex microbial community, whose importance for host health is becoming increasingly clear.
26167280	4	69	theme	nursing	632:638	arg1	pigs					640:643	nursing pigs	632:643	nursing pigs	632:643	However, there is a paucity of data examining the gut microbiome in nursing pigs from birth through weaning using modern molecular methods and fewer experimental studies that examine the impact of diet on these microbial communities.
26167280	4	70	from	birth	650:654	arg1	microbiome					618:627	the gut microbiome	610:627	the gut microbiome in nursing pigs from birth	610:654	However, there is a paucity of data examining the gut microbiome in nursing pigs from birth through weaning using modern molecular methods and fewer experimental studies that examine the impact of diet on these microbial communities.
26167280	9	71	theme	weaned	1526:1531	arg1	animals					1533:1539	weaned animals	1526:1539	weaned animals	1526:1539	In contrast, the bacterial metagenome of weaned animals was enriched in functional pathways involved in plant glycan deconstruction and consumption.
26167280	1	72	theme	microbial	131:139	arg1	community					141:149	a complex microbial community	121:149	a complex microbial community	121:149	BACKGROUND The newborn mammal is rapidly colonized by a complex microbial community, whose importance for host health is becoming increasingly clear.
26167280	8	73	theme	host	1410:1413	arg1	animal					1415:1420	the host animal	1406:1420	the host animal	1406:1420	Predicted enzymes active on milk-derived glycans that are otherwise indigestible to the host animal were enriched in the microbial metagenome of milk-fed animals.
26167280	3	74	theme	pig	496:498	arg1	similarity					482:491	physiological and compositional similarity	450:491	physiological and compositional similarity of pig and human milk	450:513	Pigs present an attractive model for nursing humans, given physiological and compositional similarity of pig and human milk and the utility of pigs in experimental studies.
26167280	3	74	theme	pig	496:498	arg1	utility					523:529	the utility	519:529	the utility of pigs in experimental studies	519:561	Pigs present an attractive model for nursing humans, given physiological and compositional similarity of pig and human milk and the utility of pigs in experimental studies.
24290815	3	0	from	determinant	461:471	arg1	plants					514:519	cheese-manufacturing plants	493:519	cheese-manufacturing plants	493:519	Cheese yield is a crucial determinant of profitability in cheese-manufacturing plants; therefore, different methods have been developed to increase it.
24290815	11	1	theme	bacteria	1483:1490	arg1	Incorporation					1452:1464	Incorporation	1452:1464	Incorporation of EPS-producing bacteria in the manufacture of cheese	1452:1519	Incorporation of EPS-producing bacteria in the manufacture of cheese increased the moisture content and water activity.
24290815	9	2	theme	electron	1354:1361	arg1	microscopy					1372:1381	electron scanning microscopy	1354:1381	electron scanning microscopy	1354:1381	Micrographs were obtained by electron scanning microscopy.
24290815	4	3	theme	cheese	628:633	arg1	model					635:639	a miniature Chihuahua-type cheese model	601:639	a miniature Chihuahua-type cheese model	601:639	In this work, a miniature Chihuahua-type cheese model was used to assess the effect of a phospholipase A1 (PL-A1) and exopolysaccharide (EPS)-producing bacteria (separately and in combination) on the yield, microstructure, and texture of cheese.
24290815	5	4	with	cheese	940:945	arg1	PL-A1					957:961	PL-A1	957:961	PL-A1	957:961	Four different cheeses were manufactured: cheese made with PL-A1, cheese made with EPS-producing bacteria, cheese with both PL-A1 and EPS-producing bacteria, and a cheese control without PL-A1 or EPS-producing bacteria.
24290815	5	4	with	cheese	940:945	arg1	bacteria					981:988	EPS-producing bacteria	967:988	EPS-producing bacteria	967:988	Four different cheeses were manufactured: cheese made with PL-A1, cheese made with EPS-producing bacteria, cheese with both PL-A1 and EPS-producing bacteria, and a cheese control without PL-A1 or EPS-producing bacteria.
24290815	1	5	theme	Mennonite	194:202	arg1	cheese					204:209	Mennonite cheese	194:209	Mennonite cheese	194:209	Chihuahua cheese or Mennonite cheese is one of the most popular and consumed cheeses in Mexico and by the Hispanic community in the United States.
24290815	6	6	theme	cheese	1083:1088	arg1	analysis					1071:1078	The compositional analysis	1053:1078	The compositional analysis of cheese	1053:1088	The compositional analysis of cheese was carried out using methods of AOAC International (Washington, DC).
24290815	13	7	theme	cheese	1689:1694	arg1	yield					1696:1700	the cheese yield	1685:1700	the cheese yield	1685:1700	The use of EPS alone improved the cheese yield by increasing water and fat retention, but also caused a negative effect on the texture and flavor of Chihuahua cheese.
24290815	15	8	with	cheeses	1956:1962	arg1	texture					1989:1995	texture	1989:1995	texture	1989:1995	The cheeses with the best flavor and texture were those manufactured with PL-A1 and the cheeses manufactured with the combination of PL-A1 and EPS-producing culture.
24290815	15	8	with	cheeses	1956:1962	arg1	flavor					1978:1983	flavor	1978:1983	flavor	1978:1983	The cheeses with the best flavor and texture were those manufactured with PL-A1 and the cheeses manufactured with the combination of PL-A1 and EPS-producing culture.
24290815	0	9	theme	phospholipase	115:127	arg1	A1					129:130	a phospholipase A1	113:130	a phospholipase A1	113:130	Assessing the yield, microstructure, and texture properties of miniature Chihuahua-type cheese manufactured with a phospholipase A1 and exopolysaccharide-producing bacteria.
24290815	3	10	theme	different	533:541	arg1	methods					543:549	different methods	533:549	different methods	533:549	Cheese yield is a crucial determinant of profitability in cheese-manufacturing plants; therefore, different methods have been developed to increase it.
24290815	4	11	theme	miniature	603:611	arg1	model					635:639	a miniature Chihuahua-type cheese model	601:639	a miniature Chihuahua-type cheese model	601:639	In this work, a miniature Chihuahua-type cheese model was used to assess the effect of a phospholipase A1 (PL-A1) and exopolysaccharide (EPS)-producing bacteria (separately and in combination) on the yield, microstructure, and texture of cheese.
24290815	13	12	theme	EPS	1666:1668	arg1	use					1659:1661	The use	1655:1661	The use of EPS	1655:1668	The use of EPS alone improved the cheese yield by increasing water and fat retention, but also caused a negative effect on the texture and flavor of Chihuahua cheese.
24290815	5	13	with	PL-A1	892:896	arg1	PL-A1					957:961	PL-A1	957:961	PL-A1	957:961	Four different cheeses were manufactured: cheese made with PL-A1, cheese made with EPS-producing bacteria, cheese with both PL-A1 and EPS-producing bacteria, and a cheese control without PL-A1 or EPS-producing bacteria.
24290815	5	13	with	PL-A1	892:896	arg1	bacteria					981:988	EPS-producing bacteria	967:988	EPS-producing bacteria	967:988	Four different cheeses were manufactured: cheese made with PL-A1, cheese made with EPS-producing bacteria, cheese with both PL-A1 and EPS-producing bacteria, and a cheese control without PL-A1 or EPS-producing bacteria.
24290815	1	14	from	cheeses	251:257	arg1	Mexico					262:267	Mexico	262:267	Mexico	262:267	Chihuahua cheese or Mennonite cheese is one of the most popular and consumed cheeses in Mexico and by the Hispanic community in the United States.
24290815	5	15	theme	different	838:846	arg1	cheeses					848:854	Four different cheeses	833:854	Four different cheeses	833:854	Four different cheeses were manufactured: cheese made with PL-A1, cheese made with EPS-producing bacteria, cheese with both PL-A1 and EPS-producing bacteria, and a cheese control without PL-A1 or EPS-producing bacteria.
24290815	6	16	theme	International	1128:1140	arg1	methods					1112:1118	methods	1112:1118	methods of AOAC International (Washington, DC)	1112:1157	The compositional analysis of cheese was carried out using methods of AOAC International (Washington, DC).
24290815	4	17	theme	phospholipase	676:688	arg1	PL-A1					694:698	PL-A1	694:698	PL-A1	694:698	In this work, a miniature Chihuahua-type cheese model was used to assess the effect of a phospholipase A1 (PL-A1) and exopolysaccharide (EPS)-producing bacteria (separately and in combination) on the yield, microstructure, and texture of cheese.
24290815	4	17	theme	phospholipase	676:688	arg1	A1					690:691	a phospholipase A1	674:691	a phospholipase A1 (PL-A1)	674:699	In this work, a miniature Chihuahua-type cheese model was used to assess the effect of a phospholipase A1 (PL-A1) and exopolysaccharide (EPS)-producing bacteria (separately and in combination) on the yield, microstructure, and texture of cheese.
24290815	5	18	theme	EPS-producing	967:979	arg1	bacteria					981:988	EPS-producing bacteria	967:988	EPS-producing bacteria	967:988	Four different cheeses were manufactured: cheese made with PL-A1, cheese made with EPS-producing bacteria, cheese with both PL-A1 and EPS-producing bacteria, and a cheese control without PL-A1 or EPS-producing bacteria.
24290815	6	19	dep	International	1128:1140	arg1	Washington					1143:1152	Washington	1143:1152	Washington	1143:1152	The compositional analysis of cheese was carried out using methods of AOAC International (Washington, DC).
24290815	6	19	dep	International	1128:1140	arg1	DC					1155:1156	DC	1155:1156	DC	1155:1156	The compositional analysis of cheese was carried out using methods of AOAC International (Washington, DC).
24290815	4	20	dep	bacteria	739:746	arg1	and					760:762	and	760:762	and	760:762	In this work, a miniature Chihuahua-type cheese model was used to assess the effect of a phospholipase A1 (PL-A1) and exopolysaccharide (EPS)-producing bacteria (separately and in combination) on the yield, microstructure, and texture of cheese.
24290815	5	21	theme	EPS-producing	1029:1041	arg1	bacteria					1043:1050	EPS-producing bacteria	1029:1050	EPS-producing bacteria	1029:1050	Four different cheeses were manufactured: cheese made with PL-A1, cheese made with EPS-producing bacteria, cheese with both PL-A1 and EPS-producing bacteria, and a cheese control without PL-A1 or EPS-producing bacteria.
24290815	8	22	theme	Texture	1248:1254	arg1	analyses					1264:1271	Texture profile analyses	1248:1271	Texture profile analyses of cheeses	1248:1282	Texture profile analyses of cheeses were performed using a texture analyzer.
24290815	11	23	theme	EPS-producing	1469:1481	arg1	bacteria					1483:1490	EPS-producing bacteria	1469:1490	EPS-producing bacteria	1469:1490	Incorporation of EPS-producing bacteria in the manufacture of cheese increased the moisture content and water activity.
24290815	0	24	theme	miniature	63:71	arg1	cheese					88:93	miniature Chihuahua-type cheese	63:93	miniature Chihuahua-type cheese manufactured with a phospholipase A1 and exopolysaccharide-producing bacteria	63:171	Assessing the yield, microstructure, and texture properties of miniature Chihuahua-type cheese manufactured with a phospholipase A1 and exopolysaccharide-producing bacteria.
24290815	15	25	theme	EPS-producing	2095:2107	arg1	culture					2109:2115	EPS-producing culture	2095:2115	EPS-producing culture	2095:2115	The cheeses with the best flavor and texture were those manufactured with PL-A1 and the cheeses manufactured with the combination of PL-A1 and EPS-producing culture.
24290815	12	26	theme	cheese	1641:1646	arg1	yield					1648:1652	cheese yield	1641:1652	cheese yield	1641:1652	In contrast, the addition of PL-A1 did not increase fat retention or cheese yield.
24290815	4	27	theme	exopolysaccharide	705:721	arg1	bacteria					739:746	a phospholipase A1 (PL-A1) and exopolysaccharide (EPS)-producing bacteria	674:746	a phospholipase A1 (PL-A1) and exopolysaccharide (EPS)-producing bacteria (separately and in combination)	674:778	In this work, a miniature Chihuahua-type cheese model was used to assess the effect of a phospholipase A1 (PL-A1) and exopolysaccharide (EPS)-producing bacteria (separately and in combination) on the yield, microstructure, and texture of cheese.
24290815	13	28	theme	cheese	1814:1819	arg1	flavor					1794:1799	flavor	1794:1799	flavor	1794:1799	The use of EPS alone improved the cheese yield by increasing water and fat retention, but also caused a negative effect on the texture and flavor of Chihuahua cheese.
24290815	13	28	theme	cheese	1814:1819	arg1	texture					1782:1788	texture	1782:1788	texture	1782:1788	The use of EPS alone improved the cheese yield by increasing water and fat retention, but also caused a negative effect on the texture and flavor of Chihuahua cheese.
24290815	0	29	theme	cheese	88:93	arg1	properties					49:58	the yield, microstructure, and texture properties	10:58	the yield, microstructure, and texture properties of miniature Chihuahua-type cheese manufactured with a phospholipase A1 and exopolysaccharide-producing bacteria	10:171	Assessing the yield, microstructure, and texture properties of miniature Chihuahua-type cheese manufactured with a phospholipase A1 and exopolysaccharide-producing bacteria.
24290815	11	30	from	Incorporation	1452:1464	arg1	manufacture					1499:1509	the manufacture	1495:1509	the manufacture of cheese	1495:1519	Incorporation of EPS-producing bacteria in the manufacture of cheese increased the moisture content and water activity.
24290815	10	31	theme	sensorial	1412:1420	arg1	analysis					1422:1429	sensorial analysis	1412:1429	sensorial analysis using ranking tests	1412:1449	Fifty panelists carried out sensorial analysis using ranking tests.
24290815	8	32	theme	cheeses	1276:1282	arg1	analyses					1264:1271	Texture profile analyses	1248:1271	Texture profile analyses of cheeses	1248:1282	Texture profile analyses of cheeses were performed using a texture analyzer.
24290815	14	33	with	combination	1859:1869	arg1	PL-A1					1876:1880	PL-A1	1876:1880	PL-A1	1876:1880	The use of EPS-producing bacteria in combination with PL-A1 improved the cheese yield and increased the moisture and fat content.
24290815	4	34	used	used	645:648	arg2	model					635:639	a miniature Chihuahua-type cheese model	601:639	a miniature Chihuahua-type cheese model	601:639	In this work, a miniature Chihuahua-type cheese model was used to assess the effect of a phospholipase A1 (PL-A1) and exopolysaccharide (EPS)-producing bacteria (separately and in combination) on the yield, microstructure, and texture of cheese.
24290815	0	35	theme	yield	14:18	arg1	properties					49:58	the yield, microstructure, and texture properties	10:58	the yield, microstructure, and texture properties of miniature Chihuahua-type cheese manufactured with a phospholipase A1 and exopolysaccharide-producing bacteria	10:171	Assessing the yield, microstructure, and texture properties of miniature Chihuahua-type cheese manufactured with a phospholipase A1 and exopolysaccharide-producing bacteria.
24290815	14	36	theme	cheese	1895:1900	arg1	yield					1902:1906	the cheese yield	1891:1906	the cheese yield	1891:1906	The use of EPS-producing bacteria in combination with PL-A1 improved the cheese yield and increased the moisture and fat content.
24290815	2	37	dep	9.5	397:399	arg1	to					394:395	to	394:395	to	394:395	According to local producers the yield of Chihuahua cheese ranges from 9 to 9.5 kg of cheese from 100 kg of milk.
24290815	0	38	theme	microstructure	21:34	arg1	properties					49:58	the yield, microstructure, and texture properties	10:58	the yield, microstructure, and texture properties of miniature Chihuahua-type cheese manufactured with a phospholipase A1 and exopolysaccharide-producing bacteria	10:171	Assessing the yield, microstructure, and texture properties of miniature Chihuahua-type cheese manufactured with a phospholipase A1 and exopolysaccharide-producing bacteria.
24290815	13	39	from	effect	1768:1773	arg1	flavor					1794:1799	flavor	1794:1799	flavor	1794:1799	The use of EPS alone improved the cheese yield by increasing water and fat retention, but also caused a negative effect on the texture and flavor of Chihuahua cheese.
24290815	13	39	from	effect	1768:1773	arg1	texture					1782:1788	texture	1782:1788	texture	1782:1788	The use of EPS alone improved the cheese yield by increasing water and fat retention, but also caused a negative effect on the texture and flavor of Chihuahua cheese.
24290815	2	40	theme	milk	429:432	arg1	kg					423:424	100 kg	419:424	100 kg of milk	419:432	According to local producers the yield of Chihuahua cheese ranges from 9 to 9.5 kg of cheese from 100 kg of milk.
24290815	11	41	theme	water	1556:1560	arg1	activity					1562:1569	water activity	1556:1569	water activity	1556:1569	Incorporation of EPS-producing bacteria in the manufacture of cheese increased the moisture content and water activity.
24290815	15	42	theme	PL-A1	2085:2089	arg1	combination					2070:2080	the combination	2066:2080	the combination of PL-A1 and EPS-producing culture	2066:2115	The cheeses with the best flavor and texture were those manufactured with PL-A1 and the cheeses manufactured with the combination of PL-A1 and EPS-producing culture.
24290815	4	43	theme	-producing	728:737	arg1	bacteria					739:746	a phospholipase A1 (PL-A1) and exopolysaccharide (EPS)-producing bacteria	674:746	a phospholipase A1 (PL-A1) and exopolysaccharide (EPS)-producing bacteria (separately and in combination)	674:778	In this work, a miniature Chihuahua-type cheese model was used to assess the effect of a phospholipase A1 (PL-A1) and exopolysaccharide (EPS)-producing bacteria (separately and in combination) on the yield, microstructure, and texture of cheese.
24290815	1	44	theme	popular	230:236	arg1	cheeses					251:257	the most popular and consumed cheeses	221:257	the most popular and consumed cheeses in Mexico and by the Hispanic community in the United States	221:318	Chihuahua cheese or Mennonite cheese is one of the most popular and consumed cheeses in Mexico and by the Hispanic community in the United States.
24290815	13	45	dep	water	1716:1720	arg1	retention					1730:1738	retention	1730:1738	retention	1730:1738	The use of EPS alone improved the cheese yield by increasing water and fat retention, but also caused a negative effect on the texture and flavor of Chihuahua cheese.
24290815	3	46	theme	profitability	476:488	arg1	yield					442:446	Cheese yield	435:446	Cheese yield	435:446	Cheese yield is a crucial determinant of profitability in cheese-manufacturing plants; therefore, different methods have been developed to increase it.
24290815	3	46	theme	profitability	476:488	arg1	determinant					461:471	a crucial determinant	451:471	a crucial determinant of profitability in cheese-manufacturing plants	451:519	Cheese yield is a crucial determinant of profitability in cheese-manufacturing plants; therefore, different methods have been developed to increase it.
24290815	4	47	from	effect	664:669	arg1	microstructure					794:807	microstructure	794:807	microstructure	794:807	In this work, a miniature Chihuahua-type cheese model was used to assess the effect of a phospholipase A1 (PL-A1) and exopolysaccharide (EPS)-producing bacteria (separately and in combination) on the yield, microstructure, and texture of cheese.
24290815	4	47	from	effect	664:669	arg1	texture					814:820	texture	814:820	texture	814:820	In this work, a miniature Chihuahua-type cheese model was used to assess the effect of a phospholipase A1 (PL-A1) and exopolysaccharide (EPS)-producing bacteria (separately and in combination) on the yield, microstructure, and texture of cheese.
24290815	4	47	from	effect	664:669	arg1	yield					787:791	the yield	783:791	the yield	783:791	In this work, a miniature Chihuahua-type cheese model was used to assess the effect of a phospholipase A1 (PL-A1) and exopolysaccharide (EPS)-producing bacteria (separately and in combination) on the yield, microstructure, and texture of cheese.
24290815	1	48	theme	consumed	242:249	arg1	cheeses					251:257	the most popular and consumed cheeses	221:257	the most popular and consumed cheeses in Mexico and by the Hispanic community in the United States	221:318	Chihuahua cheese or Mennonite cheese is one of the most popular and consumed cheeses in Mexico and by the Hispanic community in the United States.
24290815	14	49	theme	EPS-producing	1833:1845	arg1	bacteria					1847:1854	EPS-producing bacteria	1833:1854	EPS-producing bacteria	1833:1854	The use of EPS-producing bacteria in combination with PL-A1 improved the cheese yield and increased the moisture and fat content.
24290815	7	50	theme	actual	1164:1169	arg1	yield					1171:1175	The actual yield	1160:1175	The actual yield	1160:1175	The actual yield and moisture-adjusted yield were calculated for all cheese treatments.
24290815	1	51	from	community	289:297	arg1	States					313:318	the United States	302:318	the United States	302:318	Chihuahua cheese or Mennonite cheese is one of the most popular and consumed cheeses in Mexico and by the Hispanic community in the United States.
24290815	3	52	theme	cheese-manufacturing	493:512	arg1	plants					514:519	cheese-manufacturing plants	493:519	cheese-manufacturing plants	493:519	Cheese yield is a crucial determinant of profitability in cheese-manufacturing plants; therefore, different methods have been developed to increase it.
24290815	9	53	theme	scanning	1363:1370	arg1	microscopy					1372:1381	electron scanning microscopy	1354:1381	electron scanning microscopy	1354:1381	Micrographs were obtained by electron scanning microscopy.
24290815	4	54	theme	Chihuahua-type	613:626	arg1	model					635:639	a miniature Chihuahua-type cheese model	601:639	a miniature Chihuahua-type cheese model	601:639	In this work, a miniature Chihuahua-type cheese model was used to assess the effect of a phospholipase A1 (PL-A1) and exopolysaccharide (EPS)-producing bacteria (separately and in combination) on the yield, microstructure, and texture of cheese.
24290815	14	55	from	use	1826:1828	arg1	combination					1859:1869	combination	1859:1869	combination with PL-A1	1859:1880	The use of EPS-producing bacteria in combination with PL-A1 improved the cheese yield and increased the moisture and fat content.
24290815	11	56	theme	cheese	1514:1519	arg1	manufacture					1499:1509	the manufacture	1495:1509	the manufacture of cheese	1495:1519	Incorporation of EPS-producing bacteria in the manufacture of cheese increased the moisture content and water activity.
24290815	2	57	theme	Chihuahua	363:371	arg1	cheese					373:378	Chihuahua cheese	363:378	Chihuahua cheese	363:378	According to local producers the yield of Chihuahua cheese ranges from 9 to 9.5 kg of cheese from 100 kg of milk.
24290815	6	58	theme	compositional	1057:1069	arg1	analysis					1071:1078	The compositional analysis	1053:1078	The compositional analysis of cheese	1053:1088	The compositional analysis of cheese was carried out using methods of AOAC International (Washington, DC).
24290815	2	59	theme	local	334:338	arg1	producers					340:348	local producers	334:348	local producers	334:348	According to local producers the yield of Chihuahua cheese ranges from 9 to 9.5 kg of cheese from 100 kg of milk.
24290815	0	60	theme	exopolysaccharide-producing	136:162	arg1	bacteria					164:171	exopolysaccharide-producing bacteria	136:171	exopolysaccharide-producing bacteria	136:171	Assessing the yield, microstructure, and texture properties of miniature Chihuahua-type cheese manufactured with a phospholipase A1 and exopolysaccharide-producing bacteria.
24290815	5	61	theme	cheese	997:1002	arg1	control					1004:1010	a cheese control	995:1010	a cheese control without PL-A1 or EPS-producing bacteria	995:1050	Four different cheeses were manufactured: cheese made with PL-A1, cheese made with EPS-producing bacteria, cheese with both PL-A1 and EPS-producing bacteria, and a cheese control without PL-A1 or EPS-producing bacteria.
24290815	4	62	theme	cheese	825:830	arg1	microstructure					794:807	microstructure	794:807	microstructure	794:807	In this work, a miniature Chihuahua-type cheese model was used to assess the effect of a phospholipase A1 (PL-A1) and exopolysaccharide (EPS)-producing bacteria (separately and in combination) on the yield, microstructure, and texture of cheese.
24290815	4	62	theme	cheese	825:830	arg1	texture					814:820	texture	814:820	texture	814:820	In this work, a miniature Chihuahua-type cheese model was used to assess the effect of a phospholipase A1 (PL-A1) and exopolysaccharide (EPS)-producing bacteria (separately and in combination) on the yield, microstructure, and texture of cheese.
24290815	4	62	theme	cheese	825:830	arg1	yield					787:791	the yield	783:791	the yield	783:791	In this work, a miniature Chihuahua-type cheese model was used to assess the effect of a phospholipase A1 (PL-A1) and exopolysaccharide (EPS)-producing bacteria (separately and in combination) on the yield, microstructure, and texture of cheese.
24290815	15	63	theme	best	1973:1976	arg1	flavor					1978:1983	flavor	1978:1983	flavor	1978:1983	The cheeses with the best flavor and texture were those manufactured with PL-A1 and the cheeses manufactured with the combination of PL-A1 and EPS-producing culture.
24290815	5	64	with	control	1004:1010	arg1	PL-A1					957:961	PL-A1	957:961	PL-A1	957:961	Four different cheeses were manufactured: cheese made with PL-A1, cheese made with EPS-producing bacteria, cheese with both PL-A1 and EPS-producing bacteria, and a cheese control without PL-A1 or EPS-producing bacteria.
24290815	5	64	with	control	1004:1010	arg1	bacteria					981:988	EPS-producing bacteria	967:988	EPS-producing bacteria	967:988	Four different cheeses were manufactured: cheese made with PL-A1, cheese made with EPS-producing bacteria, cheese with both PL-A1 and EPS-producing bacteria, and a cheese control without PL-A1 or EPS-producing bacteria.
24290815	15	65	theme	culture	2109:2115	arg1	combination					2070:2080	the combination	2066:2080	the combination of PL-A1 and EPS-producing culture	2066:2115	The cheeses with the best flavor and texture were those manufactured with PL-A1 and the cheeses manufactured with the combination of PL-A1 and EPS-producing culture.
24290815	0	66	theme	Chihuahua-type	73:86	arg1	cheese					88:93	miniature Chihuahua-type cheese	63:93	miniature Chihuahua-type cheese manufactured with a phospholipase A1 and exopolysaccharide-producing bacteria	63:171	Assessing the yield, microstructure, and texture properties of miniature Chihuahua-type cheese manufactured with a phospholipase A1 and exopolysaccharide-producing bacteria.
24290815	1	67	theme	Chihuahua	174:182	arg1	cheese					184:189	Chihuahua cheese	174:189	Chihuahua cheese	174:189	Chihuahua cheese or Mennonite cheese is one of the most popular and consumed cheeses in Mexico and by the Hispanic community in the United States.
24290815	12	68	theme	fat	1624:1626	arg1	retention					1628:1636	fat retention	1624:1636	fat retention	1624:1636	In contrast, the addition of PL-A1 did not increase fat retention or cheese yield.
24290815	13	69	theme	Chihuahua	1804:1812	arg1	cheese					1814:1819	Chihuahua cheese	1804:1819	Chihuahua cheese	1804:1819	The use of EPS alone improved the cheese yield by increasing water and fat retention, but also caused a negative effect on the texture and flavor of Chihuahua cheese.
24290815	14	70	theme	fat	1939:1941	arg1	content					1943:1949	the moisture and fat content	1922:1949	content	1943:1949	The use of EPS-producing bacteria in combination with PL-A1 improved the cheese yield and increased the moisture and fat content.
24290815	8	71	theme	profile	1256:1262	arg1	analyses					1264:1271	Texture profile analyses	1248:1271	Texture profile analyses of cheeses	1248:1282	Texture profile analyses of cheeses were performed using a texture analyzer.
24290815	14	72	theme	moisture	1926:1933	arg1	content					1943:1949	the moisture and fat content	1922:1949	content	1943:1949	The use of EPS-producing bacteria in combination with PL-A1 improved the cheese yield and increased the moisture and fat content.
24290815	12	73	theme	PL-A1	1601:1605	arg1	addition					1589:1596	the addition	1585:1596	the addition of PL-A1	1585:1605	In contrast, the addition of PL-A1 did not increase fat retention or cheese yield.
24290815	5	74	theme	EPS-producing	916:928	arg1	bacteria					930:937	EPS-producing bacteria	916:937	EPS-producing bacteria	916:937	Four different cheeses were manufactured: cheese made with PL-A1, cheese made with EPS-producing bacteria, cheese with both PL-A1 and EPS-producing bacteria, and a cheese control without PL-A1 or EPS-producing bacteria.
24290815	7	75	theme	cheese	1229:1234	arg1	treatments					1236:1245	all cheese treatments	1225:1245	all cheese treatments	1225:1245	The actual yield and moisture-adjusted yield were calculated for all cheese treatments.
24290815	4	76	theme	A1	690:691	arg1	bacteria					739:746	a phospholipase A1 (PL-A1) and exopolysaccharide (EPS)-producing bacteria	674:746	a phospholipase A1 (PL-A1) and exopolysaccharide (EPS)-producing bacteria (separately and in combination)	674:778	In this work, a miniature Chihuahua-type cheese model was used to assess the effect of a phospholipase A1 (PL-A1) and exopolysaccharide (EPS)-producing bacteria (separately and in combination) on the yield, microstructure, and texture of cheese.
24290815	2	77	theme	cheese	373:378	arg1	yield					354:358	the yield	350:358	the yield of Chihuahua cheese	350:378	According to local producers the yield of Chihuahua cheese ranges from 9 to 9.5 kg of cheese from 100 kg of milk.
24290815	1	78	theme	Hispanic	280:287	arg1	community					289:297	the Hispanic community	276:297	the Hispanic community in the United States	276:318	Chihuahua cheese or Mennonite cheese is one of the most popular and consumed cheeses in Mexico and by the Hispanic community in the United States.
24290815	2	79	theme	cheese	407:412	arg1	kg					401:402	9 to 9.5 kg	392:402	9 to 9.5 kg of cheese	392:412	According to local producers the yield of Chihuahua cheese ranges from 9 to 9.5 kg of cheese from 100 kg of milk.
24290815	10	80	theme	ranking	1437:1443	arg1	tests					1445:1449	ranking tests	1437:1449	ranking tests	1437:1449	Fifty panelists carried out sensorial analysis using ranking tests.
24290815	13	81	theme	negative	1759:1766	arg1	effect					1768:1773	a negative effect	1757:1773	a negative effect on the texture and flavor of Chihuahua cheese	1757:1819	The use of EPS alone improved the cheese yield by increasing water and fat retention, but also caused a negative effect on the texture and flavor of Chihuahua cheese.
24290815	3	82	theme	Cheese	435:440	arg1	yield					442:446	Cheese yield	435:446	Cheese yield	435:446	Cheese yield is a crucial determinant of profitability in cheese-manufacturing plants; therefore, different methods have been developed to increase it.
24290815	3	82	theme	Cheese	435:440	arg1	determinant					461:471	a crucial determinant	451:471	a crucial determinant of profitability in cheese-manufacturing plants	451:519	Cheese yield is a crucial determinant of profitability in cheese-manufacturing plants; therefore, different methods have been developed to increase it.
24290815	5	83	with	cheese	899:904	arg1	PL-A1					957:961	PL-A1	957:961	PL-A1	957:961	Four different cheeses were manufactured: cheese made with PL-A1, cheese made with EPS-producing bacteria, cheese with both PL-A1 and EPS-producing bacteria, and a cheese control without PL-A1 or EPS-producing bacteria.
24290815	5	83	with	cheese	899:904	arg1	bacteria					981:988	EPS-producing bacteria	967:988	EPS-producing bacteria	967:988	Four different cheeses were manufactured: cheese made with PL-A1, cheese made with EPS-producing bacteria, cheese with both PL-A1 and EPS-producing bacteria, and a cheese control without PL-A1 or EPS-producing bacteria.
24290815	8	84	theme	texture	1307:1313	arg1	analyzer					1315:1322	a texture analyzer	1305:1322	a texture analyzer	1305:1322	Texture profile analyses of cheeses were performed using a texture analyzer.
24290815	0	85	theme	texture	41:47	arg1	properties					49:58	the yield, microstructure, and texture properties	10:58	the yield, microstructure, and texture properties of miniature Chihuahua-type cheese manufactured with a phospholipase A1 and exopolysaccharide-producing bacteria	10:171	Assessing the yield, microstructure, and texture properties of miniature Chihuahua-type cheese manufactured with a phospholipase A1 and exopolysaccharide-producing bacteria.
24290815	1	86	theme	United	306:311	arg1	States					313:318	the United States	302:318	the United States	302:318	Chihuahua cheese or Mennonite cheese is one of the most popular and consumed cheeses in Mexico and by the Hispanic community in the United States.
24290815	7	87	theme	moisture-adjusted	1181:1197	arg1	yield					1199:1203	moisture-adjusted yield	1181:1203	moisture-adjusted yield	1181:1203	The actual yield and moisture-adjusted yield were calculated for all cheese treatments.
24290815	11	88	theme	moisture	1535:1542	arg1	content					1544:1550	the moisture content	1531:1550	the moisture content	1531:1550	Incorporation of EPS-producing bacteria in the manufacture of cheese increased the moisture content and water activity.
24290815	3	89	theme	crucial	453:459	arg1	yield					442:446	Cheese yield	435:446	Cheese yield	435:446	Cheese yield is a crucial determinant of profitability in cheese-manufacturing plants; therefore, different methods have been developed to increase it.
24290815	3	89	theme	crucial	453:459	arg1	determinant					461:471	a crucial determinant	451:471	a crucial determinant of profitability in cheese-manufacturing plants	451:519	Cheese yield is a crucial determinant of profitability in cheese-manufacturing plants; therefore, different methods have been developed to increase it.
24290815	14	90	theme	bacteria	1847:1854	arg1	use					1826:1828	The use	1822:1828	The use of EPS-producing bacteria in combination with PL-A1	1822:1880	The use of EPS-producing bacteria in combination with PL-A1 improved the cheese yield and increased the moisture and fat content.
24290815	4	91	theme	bacteria	739:746	arg1	effect					664:669	the effect	660:669	the effect of a phospholipase A1 (PL-A1) and exopolysaccharide (EPS)-producing bacteria (separately and in combination) on the yield, microstructure, and texture of cheese	660:830	In this work, a miniature Chihuahua-type cheese model was used to assess the effect of a phospholipase A1 (PL-A1) and exopolysaccharide (EPS)-producing bacteria (separately and in combination) on the yield, microstructure, and texture of cheese.
24290815	1	92	theme	cheeses	251:257	arg1	cheeses					251:257	the most popular and consumed cheeses	221:257	the most popular and consumed cheeses in Mexico and by the Hispanic community in the United States	221:318	Chihuahua cheese or Mennonite cheese is one of the most popular and consumed cheeses in Mexico and by the Hispanic community in the United States.
24290815	1	92	theme	cheeses	251:257	arg1	one					214:216	one	214:216	one	214:216	Chihuahua cheese or Mennonite cheese is one of the most popular and consumed cheeses in Mexico and by the Hispanic community in the United States.
25079210	2	0	theme	enzymatic	536:544	arg1	hydrolysis					546:555	enzymatic hydrolysis	536:555	enzymatic hydrolysis	536:555	Mfl26 samples with elevated G-levels exhibited significantly increased hexose yields of up to 1.61-fold compared to paired samples derived from enzymatic hydrolysis, whereas Msa29 samples with high H-levels displayed increased hexose yields of only up to 1.32-fold.
25079210	1	1	dep	monolignol	357:366	arg1	G					369:369	G	369:369	G	369:369	In this study, various alkali-pretreated lignocellulose enzymatic hydrolyses were evaluated by using three standard pairs of Miscanthus accessions that showed three distinct monolignol (G, S, H) compositions.
25079210	1	1	dep	monolignol	357:366	arg1	H					375:375	H	375:375	H	375:375	In this study, various alkali-pretreated lignocellulose enzymatic hydrolyses were evaluated by using three standard pairs of Miscanthus accessions that showed three distinct monolignol (G, S, H) compositions.
25079210	1	1	dep	monolignol	357:366	arg1	S					372:372	S	372:372	S	372:372	In this study, various alkali-pretreated lignocellulose enzymatic hydrolyses were evaluated by using three standard pairs of Miscanthus accessions that showed three distinct monolignol (G, S, H) compositions.
25079210	5	2	theme	lignin	1090:1095	arg1	inhibitors					1097:1106	lignin inhibitors	1090:1106	lignin inhibitors	1090:1106	Furthermore, the G-rich samples showed more effective extraction of lignin-hemicellulose complexes than the S- and H-rich samples upon NaOH pretreatment, resulting in large removal of lignin inhibitors to yeast fermentation.
25079210	3	3	theme	paired	753:758	arg1	sample					760:765	the paired sample	749:765	the paired sample of 0.89-0.98 folds at p<0.01	749:794	In contrast, Mfl30 samples with elevated S-levels showed reduced hexose yields compared to the paired sample of 0.89-0.98 folds at p<0.01.
25079210	2	4	theme	1.32-fold	647:655	arg1	yields					626:631	increased hexose yields	609:631	increased hexose yields of only up to 1.32-fold	609:655	Mfl26 samples with elevated G-levels exhibited significantly increased hexose yields of up to 1.61-fold compared to paired samples derived from enzymatic hydrolysis, whereas Msa29 samples with high H-levels displayed increased hexose yields of only up to 1.32-fold.
25079210	5	5	theme	effective	950:958	arg1	extraction					960:969	more effective extraction	945:969	more effective extraction of lignin-hemicellulose complexes	945:1003	Furthermore, the G-rich samples showed more effective extraction of lignin-hemicellulose complexes than the S- and H-rich samples upon NaOH pretreatment, resulting in large removal of lignin inhibitors to yeast fermentation.
25079210	1	6	theme	Miscanthus	308:317	arg1	accessions					319:328	Miscanthus accessions	308:328	Miscanthus accessions	308:328	In this study, various alkali-pretreated lignocellulose enzymatic hydrolyses were evaluated by using three standard pairs of Miscanthus accessions that showed three distinct monolignol (G, S, H) compositions.
25079210	0	7	theme	diminishing	126:136	arg1	inhibitors					157:166	largely diminishing yeast fermentation inhibitors	118:166	largely diminishing yeast fermentation inhibitors	118:166	Mild alkali-pretreatment effectively extracts guaiacyl-rich lignin for high lignocellulose digestibility coupled with largely diminishing yeast fermentation inhibitors in Miscanthus.
25079210	4	8	theme	biomass	822:828	arg1	samples					830:836	only the G-rich biomass samples	806:836	only the G-rich biomass samples	806:836	Notably, only the G-rich biomass samples exhibited complete enzymatic hydrolysis under 4% NaOH pretreatment.
25079210	2	9	attach	derived	523:529	arg1	hydrolysis					546:555	enzymatic hydrolysis	536:555	enzymatic hydrolysis	536:555	Mfl26 samples with elevated G-levels exhibited significantly increased hexose yields of up to 1.61-fold compared to paired samples derived from enzymatic hydrolysis, whereas Msa29 samples with high H-levels displayed increased hexose yields of only up to 1.32-fold.
25079210	2	9	attach	derived	523:529	arg2	samples					515:521	paired samples	508:521	paired samples derived from enzymatic hydrolysis	508:555	Mfl26 samples with elevated G-levels exhibited significantly increased hexose yields of up to 1.61-fold compared to paired samples derived from enzymatic hydrolysis, whereas Msa29 samples with high H-levels displayed increased hexose yields of only up to 1.32-fold.
25079210	5	10	theme	H-rich	1021:1026	arg1	samples					1028:1034	the S- and H-rich samples	1010:1034	the S- and H-rich samples	1010:1034	Furthermore, the G-rich samples showed more effective extraction of lignin-hemicellulose complexes than the S- and H-rich samples upon NaOH pretreatment, resulting in large removal of lignin inhibitors to yeast fermentation.
25079210	1	11	theme	accessions	319:328	arg1	pairs					299:303	three standard pairs	284:303	three standard pairs of Miscanthus accessions that showed three distinct monolignol (G, S, H) compositions	284:389	In this study, various alkali-pretreated lignocellulose enzymatic hydrolyses were evaluated by using three standard pairs of Miscanthus accessions that showed three distinct monolignol (G, S, H) compositions.
25079210	3	12	theme	0.89-0.98	770:778	arg1	sample					760:765	the paired sample	749:765	the paired sample of 0.89-0.98 folds at p<0.01	749:794	In contrast, Mfl30 samples with elevated S-levels showed reduced hexose yields compared to the paired sample of 0.89-0.98 folds at p<0.01.
25079210	6	13	from	process	1251:1257	arg1	Miscanthus					1262:1271	Miscanthus	1262:1271	Miscanthus	1262:1271	Therefore, this study proposes an optimal approach for minor genetic lignin modification towards cost-effective biomass process in Miscanthus.
25079210	2	14	theme	Msa29	566:570	arg1	samples					572:578	Msa29 samples	566:578	Msa29 samples with high H-levels	566:597	Mfl26 samples with elevated G-levels exhibited significantly increased hexose yields of up to 1.61-fold compared to paired samples derived from enzymatic hydrolysis, whereas Msa29 samples with high H-levels displayed increased hexose yields of only up to 1.32-fold.
25079210	4	15	theme	NaOH	887:890	arg1	pretreatment					892:903	4% NaOH pretreatment	884:903	4% NaOH pretreatment	884:903	Notably, only the G-rich biomass samples exhibited complete enzymatic hydrolysis under 4% NaOH pretreatment.
25079210	4	16	theme	G-rich	815:820	arg1	samples					830:836	only the G-rich biomass samples	806:836	only the G-rich biomass samples	806:836	Notably, only the G-rich biomass samples exhibited complete enzymatic hydrolysis under 4% NaOH pretreatment.
25079210	1	17	theme	various	198:204	arg1	hydrolyses					249:258	various alkali-pretreated lignocellulose enzymatic hydrolyses	198:258	various alkali-pretreated lignocellulose enzymatic hydrolyses	198:258	In this study, various alkali-pretreated lignocellulose enzymatic hydrolyses were evaluated by using three standard pairs of Miscanthus accessions that showed three distinct monolignol (G, S, H) compositions.
25079210	0	18	theme	yeast	138:142	arg1	inhibitors					157:166	largely diminishing yeast fermentation inhibitors	118:166	largely diminishing yeast fermentation inhibitors	118:166	Mild alkali-pretreatment effectively extracts guaiacyl-rich lignin for high lignocellulose digestibility coupled with largely diminishing yeast fermentation inhibitors in Miscanthus.
25079210	3	19	from	p<0.01	789:794	arg1	0.89-0.98					770:778	0.89-0.98 folds	770:784	0.89-0.98 folds at p<0.01	770:794	In contrast, Mfl30 samples with elevated S-levels showed reduced hexose yields compared to the paired sample of 0.89-0.98 folds at p<0.01.
25079210	3	19	from	p<0.01	789:794	arg1	sample					760:765	the paired sample	749:765	the paired sample of 0.89-0.98 folds at p<0.01	749:794	In contrast, Mfl30 samples with elevated S-levels showed reduced hexose yields compared to the paired sample of 0.89-0.98 folds at p<0.01.
25079210	5	20	theme	NaOH	1041:1044	arg1	pretreatment					1046:1057	NaOH pretreatment	1041:1057	NaOH pretreatment	1041:1057	Furthermore, the G-rich samples showed more effective extraction of lignin-hemicellulose complexes than the S- and H-rich samples upon NaOH pretreatment, resulting in large removal of lignin inhibitors to yeast fermentation.
25079210	1	21	theme	alkali-pretreated	206:222	arg1	hydrolyses					249:258	various alkali-pretreated lignocellulose enzymatic hydrolyses	198:258	various alkali-pretreated lignocellulose enzymatic hydrolyses	198:258	In this study, various alkali-pretreated lignocellulose enzymatic hydrolyses were evaluated by using three standard pairs of Miscanthus accessions that showed three distinct monolignol (G, S, H) compositions.
25079210	3	22	from	sample	760:765	arg1	p<0.01					789:794	p<0.01	789:794	p<0.01	789:794	In contrast, Mfl30 samples with elevated S-levels showed reduced hexose yields compared to the paired sample of 0.89-0.98 folds at p<0.01.
25079210	6	23	theme	cost-effective	1228:1241	arg1	process					1251:1257	cost-effective biomass process	1228:1257	cost-effective biomass process in Miscanthus	1228:1271	Therefore, this study proposes an optimal approach for minor genetic lignin modification towards cost-effective biomass process in Miscanthus.
25079210	1	24	theme	lignocellulose	224:237	arg1	hydrolyses					249:258	various alkali-pretreated lignocellulose enzymatic hydrolyses	198:258	various alkali-pretreated lignocellulose enzymatic hydrolyses	198:258	In this study, various alkali-pretreated lignocellulose enzymatic hydrolyses were evaluated by using three standard pairs of Miscanthus accessions that showed three distinct monolignol (G, S, H) compositions.
25079210	0	25	theme	Mild	0:3	arg1	alkali-pretreatment					5:23	Mild alkali-pretreatment	0:23	Mild alkali-pretreatment	0:23	Mild alkali-pretreatment effectively extracts guaiacyl-rich lignin for high lignocellulose digestibility coupled with largely diminishing yeast fermentation inhibitors in Miscanthus.
25079210	3	26	theme	reduced	715:721	arg1	yields					730:735	reduced hexose yields	715:735	reduced hexose yields	715:735	In contrast, Mfl30 samples with elevated S-levels showed reduced hexose yields compared to the paired sample of 0.89-0.98 folds at p<0.01.
25079210	5	27	theme	inhibitors	1097:1106	arg1	removal					1079:1085	large removal	1073:1085	large removal of lignin inhibitors to yeast fermentation	1073:1128	Furthermore, the G-rich samples showed more effective extraction of lignin-hemicellulose complexes than the S- and H-rich samples upon NaOH pretreatment, resulting in large removal of lignin inhibitors to yeast fermentation.
25079210	3	28	with	samples	677:683	arg1	S-levels					699:706	elevated S-levels	690:706	elevated S-levels	690:706	In contrast, Mfl30 samples with elevated S-levels showed reduced hexose yields compared to the paired sample of 0.89-0.98 folds at p<0.01.
25079210	1	29	theme	enzymatic	239:247	arg1	hydrolyses					249:258	various alkali-pretreated lignocellulose enzymatic hydrolyses	198:258	various alkali-pretreated lignocellulose enzymatic hydrolyses	198:258	In this study, various alkali-pretreated lignocellulose enzymatic hydrolyses were evaluated by using three standard pairs of Miscanthus accessions that showed three distinct monolignol (G, S, H) compositions.
25079210	2	30	theme	increased	609:617	arg1	yields					626:631	increased hexose yields	609:631	increased hexose yields of only up to 1.32-fold	609:655	Mfl26 samples with elevated G-levels exhibited significantly increased hexose yields of up to 1.61-fold compared to paired samples derived from enzymatic hydrolysis, whereas Msa29 samples with high H-levels displayed increased hexose yields of only up to 1.32-fold.
25079210	2	31	with	samples	572:578	arg1	H-levels					590:597	high H-levels	585:597	high H-levels	585:597	Mfl26 samples with elevated G-levels exhibited significantly increased hexose yields of up to 1.61-fold compared to paired samples derived from enzymatic hydrolysis, whereas Msa29 samples with high H-levels displayed increased hexose yields of only up to 1.32-fold.
25079210	5	32	theme	S-	1014:1015	arg1	samples					1028:1034	the S- and H-rich samples	1010:1034	the S- and H-rich samples	1010:1034	Furthermore, the G-rich samples showed more effective extraction of lignin-hemicellulose complexes than the S- and H-rich samples upon NaOH pretreatment, resulting in large removal of lignin inhibitors to yeast fermentation.
25079210	5	33	theme	large	1073:1077	arg1	removal					1079:1085	large removal	1073:1085	large removal of lignin inhibitors to yeast fermentation	1073:1128	Furthermore, the G-rich samples showed more effective extraction of lignin-hemicellulose complexes than the S- and H-rich samples upon NaOH pretreatment, resulting in large removal of lignin inhibitors to yeast fermentation.
25079210	2	34	theme	up	480:481	arg1	yields					470:475	hexose yields	463:475	hexose yields of up to 1.61-fold	463:494	Mfl26 samples with elevated G-levels exhibited significantly increased hexose yields of up to 1.61-fold compared to paired samples derived from enzymatic hydrolysis, whereas Msa29 samples with high H-levels displayed increased hexose yields of only up to 1.32-fold.
25079210	1	35	theme	distinct	348:355	arg1	compositions					378:389	three distinct monolignol (G, S, H) compositions	342:389	three distinct monolignol (G, S, H) compositions	342:389	In this study, various alkali-pretreated lignocellulose enzymatic hydrolyses were evaluated by using three standard pairs of Miscanthus accessions that showed three distinct monolignol (G, S, H) compositions.
25079210	3	36	theme	elevated	690:697	arg1	S-levels					699:706	elevated S-levels	690:706	elevated S-levels	690:706	In contrast, Mfl30 samples with elevated S-levels showed reduced hexose yields compared to the paired sample of 0.89-0.98 folds at p<0.01.
25079210	6	37	theme	lignin	1200:1205	arg1	modification					1207:1218	minor genetic lignin modification	1186:1218	minor genetic lignin modification towards cost-effective biomass process in Miscanthus	1186:1271	Therefore, this study proposes an optimal approach for minor genetic lignin modification towards cost-effective biomass process in Miscanthus.
25079210	2	38	theme	paired	508:513	arg1	samples					515:521	paired samples	508:521	paired samples derived from enzymatic hydrolysis	508:555	Mfl26 samples with elevated G-levels exhibited significantly increased hexose yields of up to 1.61-fold compared to paired samples derived from enzymatic hydrolysis, whereas Msa29 samples with high H-levels displayed increased hexose yields of only up to 1.32-fold.
25079210	1	39	theme	monolignol	357:366	arg1	compositions					378:389	three distinct monolignol (G, S, H) compositions	342:389	three distinct monolignol (G, S, H) compositions	342:389	In this study, various alkali-pretreated lignocellulose enzymatic hydrolyses were evaluated by using three standard pairs of Miscanthus accessions that showed three distinct monolignol (G, S, H) compositions.
25079210	0	40	theme	fermentation	144:155	arg1	inhibitors					157:166	largely diminishing yeast fermentation inhibitors	118:166	largely diminishing yeast fermentation inhibitors	118:166	Mild alkali-pretreatment effectively extracts guaiacyl-rich lignin for high lignocellulose digestibility coupled with largely diminishing yeast fermentation inhibitors in Miscanthus.
25079210	2	41	theme	elevated	411:418	arg1	G-levels					420:427	elevated G-levels	411:427	elevated G-levels	411:427	Mfl26 samples with elevated G-levels exhibited significantly increased hexose yields of up to 1.61-fold compared to paired samples derived from enzymatic hydrolysis, whereas Msa29 samples with high H-levels displayed increased hexose yields of only up to 1.32-fold.
25079210	2	42	theme	hexose	463:468	arg1	yields					470:475	hexose yields	463:475	hexose yields of up to 1.61-fold	463:494	Mfl26 samples with elevated G-levels exhibited significantly increased hexose yields of up to 1.61-fold compared to paired samples derived from enzymatic hydrolysis, whereas Msa29 samples with high H-levels displayed increased hexose yields of only up to 1.32-fold.
25079210	0	43	theme	guaiacyl-rich	46:58	arg1	lignin					60:65	guaiacyl-rich lignin	46:65	guaiacyl-rich lignin for high lignocellulose digestibility coupled with largely diminishing yeast fermentation inhibitors in Miscanthus	46:180	Mild alkali-pretreatment effectively extracts guaiacyl-rich lignin for high lignocellulose digestibility coupled with largely diminishing yeast fermentation inhibitors in Miscanthus.
25079210	2	44	theme	high	585:588	arg1	H-levels					590:597	high H-levels	585:597	high H-levels	585:597	Mfl26 samples with elevated G-levels exhibited significantly increased hexose yields of up to 1.61-fold compared to paired samples derived from enzymatic hydrolysis, whereas Msa29 samples with high H-levels displayed increased hexose yields of only up to 1.32-fold.
25079210	2	45	dep	increased	453:461	arg1	compared					496:503	compared	496:503	compared to paired samples derived from enzymatic hydrolysis	496:555	Mfl26 samples with elevated G-levels exhibited significantly increased hexose yields of up to 1.61-fold compared to paired samples derived from enzymatic hydrolysis, whereas Msa29 samples with high H-levels displayed increased hexose yields of only up to 1.32-fold.
25079210	0	46	theme	high	71:74	arg1	digestibility					91:103	high lignocellulose digestibility	71:103	high lignocellulose digestibility coupled with largely diminishing yeast fermentation inhibitors in Miscanthus	71:180	Mild alkali-pretreatment effectively extracts guaiacyl-rich lignin for high lignocellulose digestibility coupled with largely diminishing yeast fermentation inhibitors in Miscanthus.
25079210	4	47	theme	4	884:884	arg1	%					885:885	%	885:885	%	885:885	Notably, only the G-rich biomass samples exhibited complete enzymatic hydrolysis under 4% NaOH pretreatment.
25079210	6	48	theme	genetic	1192:1198	arg1	modification					1207:1218	minor genetic lignin modification	1186:1218	minor genetic lignin modification towards cost-effective biomass process in Miscanthus	1186:1271	Therefore, this study proposes an optimal approach for minor genetic lignin modification towards cost-effective biomass process in Miscanthus.
25079210	2	49	dep	1.32-fold	647:655	arg1	to					644:645	to	644:645	to	644:645	Mfl26 samples with elevated G-levels exhibited significantly increased hexose yields of up to 1.61-fold compared to paired samples derived from enzymatic hydrolysis, whereas Msa29 samples with high H-levels displayed increased hexose yields of only up to 1.32-fold.
25079210	3	50	dep	0.89-0.98	770:778	arg1	folds					780:784	folds	780:784	folds	780:784	In contrast, Mfl30 samples with elevated S-levels showed reduced hexose yields compared to the paired sample of 0.89-0.98 folds at p<0.01.
25079210	3	51	theme	hexose	723:728	arg1	yields					730:735	reduced hexose yields	715:735	reduced hexose yields	715:735	In contrast, Mfl30 samples with elevated S-levels showed reduced hexose yields compared to the paired sample of 0.89-0.98 folds at p<0.01.
25079210	4	52	theme	%	885:885	arg1	pretreatment					892:903	4% NaOH pretreatment	884:903	4% NaOH pretreatment	884:903	Notably, only the G-rich biomass samples exhibited complete enzymatic hydrolysis under 4% NaOH pretreatment.
25079210	6	53	theme	minor	1186:1190	arg1	modification					1207:1218	minor genetic lignin modification	1186:1218	minor genetic lignin modification towards cost-effective biomass process in Miscanthus	1186:1271	Therefore, this study proposes an optimal approach for minor genetic lignin modification towards cost-effective biomass process in Miscanthus.
25079210	4	54	theme	enzymatic	857:865	arg1	hydrolysis					867:876	complete enzymatic hydrolysis	848:876	complete enzymatic hydrolysis	848:876	Notably, only the G-rich biomass samples exhibited complete enzymatic hydrolysis under 4% NaOH pretreatment.
25079210	5	55	theme	lignin-hemicellulose	974:993	arg1	complexes					995:1003	lignin-hemicellulose complexes	974:1003	lignin-hemicellulose complexes	974:1003	Furthermore, the G-rich samples showed more effective extraction of lignin-hemicellulose complexes than the S- and H-rich samples upon NaOH pretreatment, resulting in large removal of lignin inhibitors to yeast fermentation.
25079210	5	56	theme	G-rich	923:928	arg1	samples					930:936	the G-rich samples	919:936	the G-rich samples	919:936	Furthermore, the G-rich samples showed more effective extraction of lignin-hemicellulose complexes than the S- and H-rich samples upon NaOH pretreatment, resulting in large removal of lignin inhibitors to yeast fermentation.
25079210	5	57	attach	removal	1079:1085	arg2	inhibitors					1097:1106	lignin inhibitors	1090:1106	lignin inhibitors	1090:1106	Furthermore, the G-rich samples showed more effective extraction of lignin-hemicellulose complexes than the S- and H-rich samples upon NaOH pretreatment, resulting in large removal of lignin inhibitors to yeast fermentation.
25079210	5	57	attach	removal	1079:1085	arg1	fermentation					1117:1128	yeast fermentation	1111:1128	yeast fermentation	1111:1128	Furthermore, the G-rich samples showed more effective extraction of lignin-hemicellulose complexes than the S- and H-rich samples upon NaOH pretreatment, resulting in large removal of lignin inhibitors to yeast fermentation.
25079210	2	58	theme	hexose	619:624	arg1	yields					626:631	increased hexose yields	609:631	increased hexose yields of only up to 1.32-fold	609:655	Mfl26 samples with elevated G-levels exhibited significantly increased hexose yields of up to 1.61-fold compared to paired samples derived from enzymatic hydrolysis, whereas Msa29 samples with high H-levels displayed increased hexose yields of only up to 1.32-fold.
25079210	5	59	theme	yeast	1111:1115	arg1	fermentation					1117:1128	yeast fermentation	1111:1128	yeast fermentation	1111:1128	Furthermore, the G-rich samples showed more effective extraction of lignin-hemicellulose complexes than the S- and H-rich samples upon NaOH pretreatment, resulting in large removal of lignin inhibitors to yeast fermentation.
25079210	3	60	theme	Mfl30	671:675	arg1	samples					677:683	Mfl30 samples	671:683	Mfl30 samples with elevated S-levels	671:706	In contrast, Mfl30 samples with elevated S-levels showed reduced hexose yields compared to the paired sample of 0.89-0.98 folds at p<0.01.
25079210	5	61	theme	complexes	995:1003	arg1	extraction					960:969	more effective extraction	945:969	more effective extraction of lignin-hemicellulose complexes	945:1003	Furthermore, the G-rich samples showed more effective extraction of lignin-hemicellulose complexes than the S- and H-rich samples upon NaOH pretreatment, resulting in large removal of lignin inhibitors to yeast fermentation.
25079210	2	62	with	samples	398:404	arg1	G-levels					420:427	elevated G-levels	411:427	elevated G-levels	411:427	Mfl26 samples with elevated G-levels exhibited significantly increased hexose yields of up to 1.61-fold compared to paired samples derived from enzymatic hydrolysis, whereas Msa29 samples with high H-levels displayed increased hexose yields of only up to 1.32-fold.
25079210	4	63	theme	complete	848:855	arg1	hydrolysis					867:876	complete enzymatic hydrolysis	848:876	complete enzymatic hydrolysis	848:876	Notably, only the G-rich biomass samples exhibited complete enzymatic hydrolysis under 4% NaOH pretreatment.
25079210	6	64	theme	biomass	1243:1249	arg1	process					1251:1257	cost-effective biomass process	1228:1257	cost-effective biomass process in Miscanthus	1228:1271	Therefore, this study proposes an optimal approach for minor genetic lignin modification towards cost-effective biomass process in Miscanthus.
25079210	6	65	theme	optimal	1165:1171	arg1	approach					1173:1180	an optimal approach	1162:1180	an optimal approach for minor genetic lignin modification towards cost-effective biomass process in Miscanthus	1162:1271	Therefore, this study proposes an optimal approach for minor genetic lignin modification towards cost-effective biomass process in Miscanthus.
25079210	1	66	theme	standard	290:297	arg1	pairs					299:303	three standard pairs	284:303	three standard pairs of Miscanthus accessions that showed three distinct monolignol (G, S, H) compositions	284:389	In this study, various alkali-pretreated lignocellulose enzymatic hydrolyses were evaluated by using three standard pairs of Miscanthus accessions that showed three distinct monolignol (G, S, H) compositions.
25079210	0	67	theme	lignocellulose	76:89	arg1	digestibility					91:103	high lignocellulose digestibility	71:103	high lignocellulose digestibility coupled with largely diminishing yeast fermentation inhibitors in Miscanthus	71:180	Mild alkali-pretreatment effectively extracts guaiacyl-rich lignin for high lignocellulose digestibility coupled with largely diminishing yeast fermentation inhibitors in Miscanthus.
25079210	2	68	dep	exhibited	429:437	arg1	whereas					558:564	whereas	558:564	whereas	558:564	Mfl26 samples with elevated G-levels exhibited significantly increased hexose yields of up to 1.61-fold compared to paired samples derived from enzymatic hydrolysis, whereas Msa29 samples with high H-levels displayed increased hexose yields of only up to 1.32-fold.
25079210	2	68	dep	exhibited	429:437	arg1	increased					453:461	increased	453:461	exhibited significantly increased hexose yields of up to 1.61-fold compared to paired samples derived from enzymatic hydrolysis	429:555	Mfl26 samples with elevated G-levels exhibited significantly increased hexose yields of up to 1.61-fold compared to paired samples derived from enzymatic hydrolysis, whereas Msa29 samples with high H-levels displayed increased hexose yields of only up to 1.32-fold.
27974859	4	0	theme	fiber	514:518	arg1	diameters					520:528	bead-free average fiber diameters	496:528	bead-free average fiber diameters of 307±31 nm	496:541	The nanofibers exhibited bead-free average fiber diameters of 307±31 nm and VRC was released in vitro in a sustained manner.
27974859	5	1	theme	infrared	637:644	arg1	FTIR					660:663	FTIR	660:663	FTIR	660:663	The VRC nanofibers were characterized by infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and scanning electron microscopy (SEM).
27974859	5	1	theme	infrared	637:644	arg1	spectroscopy					646:657	infrared spectroscopy	637:657	infrared spectroscopy (FTIR)	637:664	The VRC nanofibers were characterized by infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and scanning electron microscopy (SEM).
27974859	1	2	theme	HPβCD	207:211	arg1	nanofibers					222:231	composited polyvinyl alcohol (PVA)/hydroxypropyl-β-cyclodextrin (HPβCD) blended nanofibers	142:231	composited polyvinyl alcohol (PVA)/hydroxypropyl-β-cyclodextrin (HPβCD) blended nanofibers	142:231	Voriconazole (VRC) incorporated in composited polyvinyl alcohol (PVA)/hydroxypropyl-β-cyclodextrin (HPβCD) blended nanofibers were produced via electrospinning for efficient ophthalmic delivery.
27974859	7	3	from	bioavailability	998:1012	arg1	tears					1031:1035	rabbit tears	1024:1035	rabbit tears	1024:1035	Compared with a VRC solution, the nanofibers significantly prolonged the half life, and increased the bioavailability of VRC in rabbit tears.
27974859	3	4	theme	%	423:423	arg1	PVA					431:433	8% (w/v) PVA	422:433	8% (w/v) PVA	422:433	The optimal solution for electrospinning consisted of 8% (w/v) PVA, 4% (w/v) HPβCD and 0.5% (w/v) VRC.
27974859	4	5	theme	average	506:512	arg1	diameters					520:528	bead-free average fiber diameters	496:528	bead-free average fiber diameters of 307±31 nm	496:541	The nanofibers exhibited bead-free average fiber diameters of 307±31 nm and VRC was released in vitro in a sustained manner.
27974859	7	6	theme	VRC	912:914	arg1	solution					916:923	a VRC solution	910:923	a VRC solution	910:923	Compared with a VRC solution, the nanofibers significantly prolonged the half life, and increased the bioavailability of VRC in rabbit tears.
27974859	2	7	theme	HPβCD	353:357	arg1	content					359:365	HPβCD content	353:365	HPβCD content	353:365	The VRC loading capacity increased with increasing HPβCD content.
27974859	5	8	theme	differential	701:712	arg1	DSC					736:738	DSC	736:738	DSC	736:738	The VRC nanofibers were characterized by infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and scanning electron microscopy (SEM).
27974859	5	8	theme	differential	701:712	arg1	calorimetry					723:733	differential scanning calorimetry	701:733	differential scanning calorimetry (DSC)	701:739	The VRC nanofibers were characterized by infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and scanning electron microscopy (SEM).
27974859	9	9	theme	VRC	1126:1128	arg1	nanofibers					1130:1139	VRC nanofibers	1126:1139	VRC nanofibers	1126:1139	VRC nanofibers are promising for ophthalmic drug delivery and further pharmacodynamics studies are needed.
27974859	5	10	theme	scanning	714:721	arg1	DSC					736:738	DSC	736:738	DSC	736:738	The VRC nanofibers were characterized by infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and scanning electron microscopy (SEM).
27974859	5	10	theme	scanning	714:721	arg1	calorimetry					723:733	differential scanning calorimetry	701:733	differential scanning calorimetry (DSC)	701:739	The VRC nanofibers were characterized by infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and scanning electron microscopy (SEM).
27974859	5	11	theme	VRC	600:602	arg1	nanofibers					604:613	The VRC nanofibers	596:613	The VRC nanofibers	596:613	The VRC nanofibers were characterized by infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and scanning electron microscopy (SEM).
27974859	8	12	located	observed	1074:1081	arg1	sac					1121:1123	the conjunctival sac	1104:1123	the conjunctival sac	1104:1123	No obvious signs of irritation were observed after application in the conjunctival sac.
27974859	8	12	located	observed	1074:1081	arg2	signs					1049:1053	No obvious signs	1038:1053	No obvious signs of irritation	1038:1067	No obvious signs of irritation were observed after application in the conjunctival sac.
27974859	3	13	theme	8	422:422	arg1	%					423:423	%	423:423	%	423:423	The optimal solution for electrospinning consisted of 8% (w/v) PVA, 4% (w/v) HPβCD and 0.5% (w/v) VRC.
27974859	3	14	dep	%	437:437	arg1	w/v					440:442	w/v	440:442	w/v	440:442	The optimal solution for electrospinning consisted of 8% (w/v) PVA, 4% (w/v) HPβCD and 0.5% (w/v) VRC.
27974859	4	15	theme	sustained	578:586	arg1	manner					588:593	a sustained manner	576:593	a sustained manner	576:593	The nanofibers exhibited bead-free average fiber diameters of 307±31 nm and VRC was released in vitro in a sustained manner.
27974859	1	16	theme	blended	214:220	arg1	nanofibers					222:231	composited polyvinyl alcohol (PVA)/hydroxypropyl-β-cyclodextrin (HPβCD) blended nanofibers	142:231	composited polyvinyl alcohol (PVA)/hydroxypropyl-β-cyclodextrin (HPβCD) blended nanofibers	142:231	Voriconazole (VRC) incorporated in composited polyvinyl alcohol (PVA)/hydroxypropyl-β-cyclodextrin (HPβCD) blended nanofibers were produced via electrospinning for efficient ophthalmic delivery.
27974859	6	17	from	ratio	858:862	arg1	nanofibers					884:893	the nanofibers	880:893	the nanofibers	880:893	The proton nuclear magnetic resonance (1H-NMR) was used to analyze the molar ratio of HPβCD/VRC in the nanofibers.
27974859	7	18	theme	rabbit	1024:1029	arg1	tears					1031:1035	rabbit tears	1024:1035	rabbit tears	1024:1035	Compared with a VRC solution, the nanofibers significantly prolonged the half life, and increased the bioavailability of VRC in rabbit tears.
27974859	0	19	theme	Ophthalmic	86:95	arg1	Delivery					97:104	Ophthalmic Delivery	86:104	Ophthalmic Delivery	86:104	Voriconazole Composited Polyvinyl Alcohol/Hydroxypropyl-β-Cyclodextrin Nanofibers for Ophthalmic Delivery.
27974859	0	20	theme	Alcohol/Hydroxypropyl-β-Cyclodextrin	34:69	arg1	Nanofibers					71:80	Polyvinyl Alcohol/Hydroxypropyl-β-Cyclodextrin Nanofibers	24:80	Polyvinyl Alcohol/Hydroxypropyl-β-Cyclodextrin Nanofibers for Ophthalmic Delivery	24:104	Voriconazole Composited Polyvinyl Alcohol/Hydroxypropyl-β-Cyclodextrin Nanofibers for Ophthalmic Delivery.
27974859	9	21	theme	ophthalmic	1159:1168	arg1	delivery					1175:1182	ophthalmic drug delivery	1159:1182	ophthalmic drug delivery	1159:1182	VRC nanofibers are promising for ophthalmic drug delivery and further pharmacodynamics studies are needed.
27974859	0	22	theme	Polyvinyl	24:32	arg1	Nanofibers					71:80	Polyvinyl Alcohol/Hydroxypropyl-β-Cyclodextrin Nanofibers	24:80	Polyvinyl Alcohol/Hydroxypropyl-β-Cyclodextrin Nanofibers for Ophthalmic Delivery	24:104	Voriconazole Composited Polyvinyl Alcohol/Hydroxypropyl-β-Cyclodextrin Nanofibers for Ophthalmic Delivery.
27974859	7	23	theme	VRC	1017:1019	arg1	bioavailability					998:1012	the bioavailability	994:1012	the bioavailability of VRC in rabbit tears	994:1035	Compared with a VRC solution, the nanofibers significantly prolonged the half life, and increased the bioavailability of VRC in rabbit tears.
27974859	9	24	theme	drug	1170:1173	arg1	delivery					1175:1182	ophthalmic drug delivery	1159:1182	ophthalmic drug delivery	1159:1182	VRC nanofibers are promising for ophthalmic drug delivery and further pharmacodynamics studies are needed.
27974859	1	25	theme	composited	142:151	arg1	PVA					172:174	PVA	172:174	PVA	172:174	Voriconazole (VRC) incorporated in composited polyvinyl alcohol (PVA)/hydroxypropyl-β-cyclodextrin (HPβCD) blended nanofibers were produced via electrospinning for efficient ophthalmic delivery.
27974859	1	25	theme	composited	142:151	arg1	alcohol					163:169	composited polyvinyl alcohol	142:169	composited polyvinyl alcohol (PVA)/hydroxypropyl-β-cyclodextrin (HPβCD) blended nanofibers	142:231	Voriconazole (VRC) incorporated in composited polyvinyl alcohol (PVA)/hydroxypropyl-β-cyclodextrin (HPβCD) blended nanofibers were produced via electrospinning for efficient ophthalmic delivery.
27974859	5	26	theme	scanning	745:752	arg1	microscopy					763:772	scanning electron microscopy	745:772	scanning electron microscopy (SEM)	745:778	The VRC nanofibers were characterized by infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and scanning electron microscopy (SEM).
27974859	5	26	theme	scanning	745:752	arg1	SEM					775:777	SEM	775:777	SEM	775:777	The VRC nanofibers were characterized by infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and scanning electron microscopy (SEM).
27974859	3	27	theme	%	458:458	arg1	VRC					466:468	0.5% (w/v) VRC	455:468	0.5% (w/v) VRC	455:468	The optimal solution for electrospinning consisted of 8% (w/v) PVA, 4% (w/v) HPβCD and 0.5% (w/v) VRC.
27974859	5	28	theme	electron	754:761	arg1	microscopy					763:772	scanning electron microscopy	745:772	scanning electron microscopy (SEM)	745:778	The VRC nanofibers were characterized by infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and scanning electron microscopy (SEM).
27974859	5	28	theme	electron	754:761	arg1	SEM					775:777	SEM	775:777	SEM	775:777	The VRC nanofibers were characterized by infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and scanning electron microscopy (SEM).
27974859	3	29	theme	4	436:436	arg1	%					437:437	%	437:437	%	437:437	The optimal solution for electrospinning consisted of 8% (w/v) PVA, 4% (w/v) HPβCD and 0.5% (w/v) VRC.
27974859	8	30	theme	obvious	1041:1047	arg1	signs					1049:1053	No obvious signs	1038:1053	No obvious signs of irritation	1038:1067	No obvious signs of irritation were observed after application in the conjunctival sac.
27974859	9	31	theme	further	1188:1194	arg1	studies					1213:1219	further pharmacodynamics studies	1188:1219	further pharmacodynamics studies	1188:1219	VRC nanofibers are promising for ophthalmic drug delivery and further pharmacodynamics studies are needed.
27974859	7	32	theme	half	969:972	arg1	life					974:977	the half life	965:977	the half life	965:977	Compared with a VRC solution, the nanofibers significantly prolonged the half life, and increased the bioavailability of VRC in rabbit tears.
27974859	1	33	theme	polyvinyl	153:161	arg1	PVA					172:174	PVA	172:174	PVA	172:174	Voriconazole (VRC) incorporated in composited polyvinyl alcohol (PVA)/hydroxypropyl-β-cyclodextrin (HPβCD) blended nanofibers were produced via electrospinning for efficient ophthalmic delivery.
27974859	1	33	theme	polyvinyl	153:161	arg1	alcohol					163:169	composited polyvinyl alcohol	142:169	composited polyvinyl alcohol (PVA)/hydroxypropyl-β-cyclodextrin (HPβCD) blended nanofibers	142:231	Voriconazole (VRC) incorporated in composited polyvinyl alcohol (PVA)/hydroxypropyl-β-cyclodextrin (HPβCD) blended nanofibers were produced via electrospinning for efficient ophthalmic delivery.
27974859	3	34	theme	%	437:437	arg1	HPβCD					445:449	4% (w/v) HPβCD	436:449	4% (w/v) HPβCD	436:449	The optimal solution for electrospinning consisted of 8% (w/v) PVA, 4% (w/v) HPβCD and 0.5% (w/v) VRC.
27974859	4	35	theme	nm	540:541	arg1	diameters					520:528	bead-free average fiber diameters	496:528	bead-free average fiber diameters of 307±31 nm	496:541	The nanofibers exhibited bead-free average fiber diameters of 307±31 nm and VRC was released in vitro in a sustained manner.
27974859	3	36	theme	optimal	372:378	arg1	solution					380:387	The optimal solution	368:387	The optimal solution for electrospinning	368:407	The optimal solution for electrospinning consisted of 8% (w/v) PVA, 4% (w/v) HPβCD and 0.5% (w/v) VRC.
27974859	9	37	theme	pharmacodynamics	1196:1211	arg1	studies					1213:1219	further pharmacodynamics studies	1188:1219	further pharmacodynamics studies	1188:1219	VRC nanofibers are promising for ophthalmic drug delivery and further pharmacodynamics studies are needed.
27974859	6	38	theme	magnetic	800:807	arg1	1H-NMR					820:825	1H-NMR	820:825	1H-NMR	820:825	The proton nuclear magnetic resonance (1H-NMR) was used to analyze the molar ratio of HPβCD/VRC in the nanofibers.
27974859	6	38	theme	magnetic	800:807	arg1	resonance					809:817	The proton nuclear magnetic resonance	781:817	The proton nuclear magnetic resonance (1H-NMR)	781:826	The proton nuclear magnetic resonance (1H-NMR) was used to analyze the molar ratio of HPβCD/VRC in the nanofibers.
27974859	1	39	theme	alcohol	163:169	arg1	nanofibers					222:231	composited polyvinyl alcohol (PVA)/hydroxypropyl-β-cyclodextrin (HPβCD) blended nanofibers	142:231	composited polyvinyl alcohol (PVA)/hydroxypropyl-β-cyclodextrin (HPβCD) blended nanofibers	142:231	Voriconazole (VRC) incorporated in composited polyvinyl alcohol (PVA)/hydroxypropyl-β-cyclodextrin (HPβCD) blended nanofibers were produced via electrospinning for efficient ophthalmic delivery.
27974859	3	40	dep	%	423:423	arg1	w/v					426:428	w/v	426:428	w/v	426:428	The optimal solution for electrospinning consisted of 8% (w/v) PVA, 4% (w/v) HPβCD and 0.5% (w/v) VRC.
27974859	6	41	theme	HPβCD/VRC	867:875	arg1	ratio					858:862	the molar ratio	848:862	the molar ratio of HPβCD/VRC in the nanofibers	848:893	The proton nuclear magnetic resonance (1H-NMR) was used to analyze the molar ratio of HPβCD/VRC in the nanofibers.
27974859	3	42	dep	%	458:458	arg1	w/v					461:463	w/v	461:463	w/v	461:463	The optimal solution for electrospinning consisted of 8% (w/v) PVA, 4% (w/v) HPβCD and 0.5% (w/v) VRC.
27974859	1	43	theme	efficient	271:279	arg1	delivery					292:299	efficient ophthalmic delivery	271:299	efficient ophthalmic delivery	271:299	Voriconazole (VRC) incorporated in composited polyvinyl alcohol (PVA)/hydroxypropyl-β-cyclodextrin (HPβCD) blended nanofibers were produced via electrospinning for efficient ophthalmic delivery.
27974859	5	44	theme	thermogravimetric	667:683	arg1	TGA					695:697	TGA	695:697	TGA	695:697	The VRC nanofibers were characterized by infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and scanning electron microscopy (SEM).
27974859	5	44	theme	thermogravimetric	667:683	arg1	analysis					685:692	thermogravimetric analysis	667:692	thermogravimetric analysis (TGA)	667:698	The VRC nanofibers were characterized by infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and scanning electron microscopy (SEM).
27974859	6	45	theme	nuclear	792:798	arg1	1H-NMR					820:825	1H-NMR	820:825	1H-NMR	820:825	The proton nuclear magnetic resonance (1H-NMR) was used to analyze the molar ratio of HPβCD/VRC in the nanofibers.
27974859	6	45	theme	nuclear	792:798	arg1	resonance					809:817	The proton nuclear magnetic resonance	781:817	The proton nuclear magnetic resonance (1H-NMR)	781:826	The proton nuclear magnetic resonance (1H-NMR) was used to analyze the molar ratio of HPβCD/VRC in the nanofibers.
27974859	2	46	theme	loading	310:316	arg1	capacity					318:325	The VRC loading capacity	302:325	The VRC loading capacity	302:325	The VRC loading capacity increased with increasing HPβCD content.
27974859	1	47	theme	ophthalmic	281:290	arg1	delivery					292:299	efficient ophthalmic delivery	271:299	efficient ophthalmic delivery	271:299	Voriconazole (VRC) incorporated in composited polyvinyl alcohol (PVA)/hydroxypropyl-β-cyclodextrin (HPβCD) blended nanofibers were produced via electrospinning for efficient ophthalmic delivery.
27974859	6	48	used	used	832:835	arg2	1H-NMR					820:825	1H-NMR	820:825	1H-NMR	820:825	The proton nuclear magnetic resonance (1H-NMR) was used to analyze the molar ratio of HPβCD/VRC in the nanofibers.
27974859	6	48	used	used	832:835	arg2	resonance					809:817	The proton nuclear magnetic resonance	781:817	The proton nuclear magnetic resonance (1H-NMR)	781:826	The proton nuclear magnetic resonance (1H-NMR) was used to analyze the molar ratio of HPβCD/VRC in the nanofibers.
27974859	6	49	theme	proton	785:790	arg1	1H-NMR					820:825	1H-NMR	820:825	1H-NMR	820:825	The proton nuclear magnetic resonance (1H-NMR) was used to analyze the molar ratio of HPβCD/VRC in the nanofibers.
27974859	6	49	theme	proton	785:790	arg1	resonance					809:817	The proton nuclear magnetic resonance	781:817	The proton nuclear magnetic resonance (1H-NMR)	781:826	The proton nuclear magnetic resonance (1H-NMR) was used to analyze the molar ratio of HPβCD/VRC in the nanofibers.
27974859	2	50	theme	VRC	306:308	arg1	capacity					318:325	The VRC loading capacity	302:325	The VRC loading capacity	302:325	The VRC loading capacity increased with increasing HPβCD content.
27974859	8	51	theme	conjunctival	1108:1119	arg1	sac					1121:1123	the conjunctival sac	1104:1123	the conjunctival sac	1104:1123	No obvious signs of irritation were observed after application in the conjunctival sac.
27974859	3	52	theme	0.5	455:457	arg1	%					458:458	%	458:458	%	458:458	The optimal solution for electrospinning consisted of 8% (w/v) PVA, 4% (w/v) HPβCD and 0.5% (w/v) VRC.
27974859	6	53	theme	molar	852:856	arg1	ratio					858:862	the molar ratio	848:862	the molar ratio of HPβCD/VRC in the nanofibers	848:893	The proton nuclear magnetic resonance (1H-NMR) was used to analyze the molar ratio of HPβCD/VRC in the nanofibers.
27974859	8	54	theme	irritation	1058:1067	arg1	signs					1049:1053	No obvious signs	1038:1053	No obvious signs of irritation	1038:1067	No obvious signs of irritation were observed after application in the conjunctival sac.
27974859	4	55	theme	bead-free	496:504	arg1	diameters					520:528	bead-free average fiber diameters	496:528	bead-free average fiber diameters of 307±31 nm	496:541	The nanofibers exhibited bead-free average fiber diameters of 307±31 nm and VRC was released in vitro in a sustained manner.
27974859	1	56	theme	/hydroxypropyl-β-cyclodextrin	176:204	arg1	nanofibers					222:231	composited polyvinyl alcohol (PVA)/hydroxypropyl-β-cyclodextrin (HPβCD) blended nanofibers	142:231	composited polyvinyl alcohol (PVA)/hydroxypropyl-β-cyclodextrin (HPβCD) blended nanofibers	142:231	Voriconazole (VRC) incorporated in composited polyvinyl alcohol (PVA)/hydroxypropyl-β-cyclodextrin (HPβCD) blended nanofibers were produced via electrospinning for efficient ophthalmic delivery.
28493885	4	0	theme	rhamnosus	544:552	arg1	isolates					529:536	16 clinical blood isolates	511:536	16 clinical blood isolates of L. rhamnosus	511:552	The aim of the study was to examine the genomic and immunological properties of 16 clinical blood isolates of L. rhamnosus and to compare them to the well-studied L. rhamnosus probiotic strain GG.
28493885	0	1	with	comparison	87:96	arg1	strain					103:108	strain GG	103:111	strain GG	103:111	Genotypic and phenotypic diversity of Lactobacillus rhamnosus clinical isolates, their comparison with strain GG and their recognition by complement system.
28493885	5	2	theme	Blood	628:632	arg1	cultures					634:641	Blood cultures	628:641	Blood cultures from bacteremic patients	628:666	Blood cultures from bacteremic patients were collected at the Helsinki University Hospital laboratory in 2005-2011 and L. rhamnosus strains were isolated and characterized by genomic sequencing.
28493885	12	3	theme	considerable	1758:1769	arg1	heterogeneity					1771:1783	considerable heterogeneity	1758:1783	considerable heterogeneity	1758:1783	These findings suggest that clinical L. rhamnosus isolates show considerable heterogeneity but are clearly different from L. rhamnosus GG at the genomic level.
28493885	7	4	dep	regulators	1031:1040	arg1	H					1049:1049	factor H	1042:1049	factor H	1042:1049	Binding of complement regulators factor H and C4bp was also determined using radioligand assays.
28493885	7	4	dep	regulators	1031:1040	arg1	regulators					1031:1040	complement regulators	1020:1040	complement regulators factor H and C4bp	1020:1058	Binding of complement regulators factor H and C4bp was also determined using radioligand assays.
28493885	7	4	dep	regulators	1031:1040	arg1	C4bp					1055:1058	C4bp	1055:1058	C4bp	1055:1058	Binding of complement regulators factor H and C4bp was also determined using radioligand assays.
28493885	11	5	theme	platelet	1627:1634	arg1	aggregation					1636:1646	platelet aggregation	1627:1646	platelet aggregation activity	1627:1655	Four out of 16 clinical blood isolates induced platelet aggregation and/or formed more biofilms than L. rhamnosus GG, which did not display platelet aggregation activity nor showed strong biofilm formation.
28493885	10	6	theme	rhamnosus	1393:1401	arg1	strains					1403:1409	All L. rhamnosus strains	1386:1409	All L. rhamnosus strains	1386:1409	All L. rhamnosus strains activated complement in serum and none of them bound complement regulators.
28493885	0	7	theme	Genotypic	0:8	arg1	diversity					25:33	Genotypic and phenotypic diversity	0:33	Genotypic and phenotypic diversity of Lactobacillus rhamnosus clinical isolates	0:78	Genotypic and phenotypic diversity of Lactobacillus rhamnosus clinical isolates, their comparison with strain GG and their recognition by complement system.
28493885	6	8	theme	strains	856:862	arg1	capacity					827:834	The capacity	823:834	The capacity of the L. rhamnosus strains to activate serum complement	823:891	The capacity of the L. rhamnosus strains to activate serum complement was studied using immunological assays for complement factor C3a and the terminal pathway complement complex (TCC).
28493885	1	9	from	body	241:244	arg1	ubiquitous					193:202	ubiquitous	193:202	ubiquitous	193:202	Lactobacillus rhamnosus strains are ubiquitous in fermented foods, and in the human body where they are commensals naturally present in the normal microbiota composition of gut, vagina and skin.
28493885	1	10	from	present	282:288	arg1	composition					315:325	the normal microbiota composition	293:325	the normal microbiota composition of gut, vagina and skin	293:349	Lactobacillus rhamnosus strains are ubiquitous in fermented foods, and in the human body where they are commensals naturally present in the normal microbiota composition of gut, vagina and skin.
28493885	12	11	theme	L.	1731:1732	arg1	isolates					1744:1751	clinical L. rhamnosus isolates	1722:1751	clinical L. rhamnosus isolates	1722:1751	These findings suggest that clinical L. rhamnosus isolates show considerable heterogeneity but are clearly different from L. rhamnosus GG at the genomic level.
28493885	11	12	theme	blood	1511:1515	arg1	isolates					1517:1524	16 clinical blood isolates	1499:1524	16 clinical blood isolates	1499:1524	Four out of 16 clinical blood isolates induced platelet aggregation and/or formed more biofilms than L. rhamnosus GG, which did not display platelet aggregation activity nor showed strong biofilm formation.
28493885	4	13	theme	L.	594:595	arg1	GG					624:625	the well-studied L. rhamnosus probiotic strain GG	577:625	the well-studied L. rhamnosus probiotic strain GG	577:625	The aim of the study was to examine the genomic and immunological properties of 16 clinical blood isolates of L. rhamnosus and to compare them to the well-studied L. rhamnosus probiotic strain GG.
28493885	6	14	theme	factor	947:952	arg1	C3a					954:956	complement factor C3a	936:956	complement factor C3a	936:956	The capacity of the L. rhamnosus strains to activate serum complement was studied using immunological assays for complement factor C3a and the terminal pathway complement complex (TCC).
28493885	4	15	theme	probiotic	607:615	arg1	GG					624:625	the well-studied L. rhamnosus probiotic strain GG	577:625	the well-studied L. rhamnosus probiotic strain GG	577:625	The aim of the study was to examine the genomic and immunological properties of 16 clinical blood isolates of L. rhamnosus and to compare them to the well-studied L. rhamnosus probiotic strain GG.
28493885	7	16	theme	regulators	1031:1040	arg1	Binding					1009:1015	Binding	1009:1015	Binding of complement regulators factor H and C4bp	1009:1058	Binding of complement regulators factor H and C4bp was also determined using radioligand assays.
28493885	6	17	theme	immunological	911:923	arg1	assays					925:930	immunological assays	911:930	immunological assays for complement factor C3a and the terminal pathway complement complex (TCC)	911:1006	The capacity of the L. rhamnosus strains to activate serum complement was studied using immunological assays for complement factor C3a and the terminal pathway complement complex (TCC).
28493885	12	18	dep	L.	1731:1732	arg1	rhamnosus					1734:1742	rhamnosus	1734:1742	rhamnosus	1734:1742	These findings suggest that clinical L. rhamnosus isolates show considerable heterogeneity but are clearly different from L. rhamnosus GG at the genomic level.
28493885	1	19	from	ubiquitous	193:202	arg1	foods					217:221	fermented foods	207:221	fermented foods	207:221	Lactobacillus rhamnosus strains are ubiquitous in fermented foods, and in the human body where they are commensals naturally present in the normal microbiota composition of gut, vagina and skin.
28493885	1	19	from	ubiquitous	193:202	arg1	body					241:244	the human body	231:244	the human body where they are commensals naturally present in the normal microbiota composition of gut, vagina and skin	231:349	Lactobacillus rhamnosus strains are ubiquitous in fermented foods, and in the human body where they are commensals naturally present in the normal microbiota composition of gut, vagina and skin.
28493885	13	20	theme	human	1916:1920	arg1	system					1933:1938	the human complement system	1912:1938	the human complement system	1912:1938	All L. rhamnosus strains are still normally recognized by the human complement system.
28493885	0	21	theme	isolates	71:78	arg1	diversity					25:33	Genotypic and phenotypic diversity	0:33	Genotypic and phenotypic diversity of Lactobacillus rhamnosus clinical isolates	0:78	Genotypic and phenotypic diversity of Lactobacillus rhamnosus clinical isolates, their comparison with strain GG and their recognition by complement system.
28493885	0	21	theme	isolates	71:78	arg1	comparison					87:96	their comparison	81:96	their comparison with strain GG	81:111	Genotypic and phenotypic diversity of Lactobacillus rhamnosus clinical isolates, their comparison with strain GG and their recognition by complement system.
28493885	0	21	theme	isolates	71:78	arg1	recognition					123:133	their recognition	117:133	their recognition by complement system	117:154	Genotypic and phenotypic diversity of Lactobacillus rhamnosus clinical isolates, their comparison with strain GG and their recognition by complement system.
28493885	9	22	theme	L.	1267:1268	arg1	strains					1280:1286	the clinical L. rhamnosus strains	1254:1286	the clinical L. rhamnosus strains	1254:1286	Genomic comparison between the clinical L. rhamnosus strains showed them to be clearly different from L. rhamnosus GG and to cluster in two distinct lineages.
28493885	5	23	theme	genomic	803:809	arg1	sequencing					811:820	genomic sequencing	803:820	genomic sequencing	803:820	Blood cultures from bacteremic patients were collected at the Helsinki University Hospital laboratory in 2005-2011 and L. rhamnosus strains were isolated and characterized by genomic sequencing.
28493885	5	24	theme	L.	747:748	arg1	strains					760:766	L. rhamnosus strains	747:766	L. rhamnosus strains	747:766	Blood cultures from bacteremic patients were collected at the Helsinki University Hospital laboratory in 2005-2011 and L. rhamnosus strains were isolated and characterized by genomic sequencing.
28493885	1	25	theme	vagina	335:340	arg1	composition					315:325	the normal microbiota composition	293:325	the normal microbiota composition of gut, vagina and skin	293:349	Lactobacillus rhamnosus strains are ubiquitous in fermented foods, and in the human body where they are commensals naturally present in the normal microbiota composition of gut, vagina and skin.
28493885	1	26	theme	rhamnosus	171:179	arg1	strains					181:187	Lactobacillus rhamnosus strains	157:187	Lactobacillus rhamnosus strains	157:187	Lactobacillus rhamnosus strains are ubiquitous in fermented foods, and in the human body where they are commensals naturally present in the normal microbiota composition of gut, vagina and skin.
28493885	6	27	theme	complement	983:992	arg1	TCC					1003:1005	TCC	1003:1005	TCC	1003:1005	The capacity of the L. rhamnosus strains to activate serum complement was studied using immunological assays for complement factor C3a and the terminal pathway complement complex (TCC).
28493885	6	27	theme	complement	983:992	arg1	complex					994:1000	the terminal pathway complement complex	962:1000	the terminal pathway complement complex (TCC)	962:1006	The capacity of the L. rhamnosus strains to activate serum complement was studied using immunological assays for complement factor C3a and the terminal pathway complement complex (TCC).
28493885	4	28	theme	genomic	471:477	arg1	properties					497:506	the genomic and immunological properties	467:506	the genomic and immunological properties of 16 clinical blood isolates of L. rhamnosus	467:552	The aim of the study was to examine the genomic and immunological properties of 16 clinical blood isolates of L. rhamnosus and to compare them to the well-studied L. rhamnosus probiotic strain GG.
28493885	1	29	theme	skin	346:349	arg1	composition					315:325	the normal microbiota composition	293:325	the normal microbiota composition of gut, vagina and skin	293:349	Lactobacillus rhamnosus strains are ubiquitous in fermented foods, and in the human body where they are commensals naturally present in the normal microbiota composition of gut, vagina and skin.
28493885	6	30	theme	terminal	966:973	arg1	TCC					1003:1005	TCC	1003:1005	TCC	1003:1005	The capacity of the L. rhamnosus strains to activate serum complement was studied using immunological assays for complement factor C3a and the terminal pathway complement complex (TCC).
28493885	6	30	theme	terminal	966:973	arg1	complex					994:1000	the terminal pathway complement complex	962:1000	the terminal pathway complement complex (TCC)	962:1006	The capacity of the L. rhamnosus strains to activate serum complement was studied using immunological assays for complement factor C3a and the terminal pathway complement complex (TCC).
28493885	0	31	theme	Lactobacillus	38:50	arg1	isolates					71:78	Lactobacillus rhamnosus clinical isolates	38:78	Lactobacillus rhamnosus clinical isolates	38:78	Genotypic and phenotypic diversity of Lactobacillus rhamnosus clinical isolates, their comparison with strain GG and their recognition by complement system.
28493885	13	32	theme	rhamnosus	1861:1869	arg1	strains					1871:1877	All L. rhamnosus strains	1854:1877	All L. rhamnosus strains	1854:1877	All L. rhamnosus strains are still normally recognized by the human complement system.
28493885	10	33	dep	serum	1435:1439	arg1	regulators					1475:1484	bound complement regulators	1458:1484	bound complement regulators	1458:1484	All L. rhamnosus strains activated complement in serum and none of them bound complement regulators.
28493885	5	34	from	2005-2011	733:741	arg1	laboratory					719:728	laboratory	719:728	laboratory	719:728	Blood cultures from bacteremic patients were collected at the Helsinki University Hospital laboratory in 2005-2011 and L. rhamnosus strains were isolated and characterized by genomic sequencing.
28493885	4	35	theme	clinical	514:521	arg1	isolates					529:536	16 clinical blood isolates	511:536	16 clinical blood isolates of L. rhamnosus	511:552	The aim of the study was to examine the genomic and immunological properties of 16 clinical blood isolates of L. rhamnosus and to compare them to the well-studied L. rhamnosus probiotic strain GG.
28493885	0	36	theme	phenotypic	14:23	arg1	diversity					25:33	Genotypic and phenotypic diversity	0:33	Genotypic and phenotypic diversity of Lactobacillus rhamnosus clinical isolates	0:78	Genotypic and phenotypic diversity of Lactobacillus rhamnosus clinical isolates, their comparison with strain GG and their recognition by complement system.
28493885	6	37	theme	rhamnosus	846:854	arg1	strains					856:862	the L. rhamnosus strains	839:862	the L. rhamnosus strains	839:862	The capacity of the L. rhamnosus strains to activate serum complement was studied using immunological assays for complement factor C3a and the terminal pathway complement complex (TCC).
28493885	4	38	theme	isolates	529:536	arg1	properties					497:506	the genomic and immunological properties	467:506	the genomic and immunological properties of 16 clinical blood isolates of L. rhamnosus	467:552	The aim of the study was to examine the genomic and immunological properties of 16 clinical blood isolates of L. rhamnosus and to compare them to the well-studied L. rhamnosus probiotic strain GG.
28493885	10	39	theme	bound	1458:1462	arg1	regulators					1475:1484	bound complement regulators	1458:1484	bound complement regulators	1458:1484	All L. rhamnosus strains activated complement in serum and none of them bound complement regulators.
28493885	1	40	theme	normal	297:302	arg1	composition					315:325	the normal microbiota composition	293:325	the normal microbiota composition of gut, vagina and skin	293:349	Lactobacillus rhamnosus strains are ubiquitous in fermented foods, and in the human body where they are commensals naturally present in the normal microbiota composition of gut, vagina and skin.
28493885	4	41	theme	immunological	483:495	arg1	properties					497:506	the genomic and immunological properties	467:506	the genomic and immunological properties of 16 clinical blood isolates of L. rhamnosus	467:552	The aim of the study was to examine the genomic and immunological properties of 16 clinical blood isolates of L. rhamnosus and to compare them to the well-studied L. rhamnosus probiotic strain GG.
28493885	11	42	theme	strong	1668:1673	arg1	formation					1683:1691	strong biofilm formation	1668:1691	strong biofilm formation	1668:1691	Four out of 16 clinical blood isolates induced platelet aggregation and/or formed more biofilms than L. rhamnosus GG, which did not display platelet aggregation activity nor showed strong biofilm formation.
28493885	1	43	theme	gut	330:332	arg1	composition					315:325	the normal microbiota composition	293:325	the normal microbiota composition of gut, vagina and skin	293:349	Lactobacillus rhamnosus strains are ubiquitous in fermented foods, and in the human body where they are commensals naturally present in the normal microbiota composition of gut, vagina and skin.
28493885	12	44	from	rhamnosus	1819:1827	arg1	different					1801:1809	different	1801:1809	different	1801:1809	These findings suggest that clinical L. rhamnosus isolates show considerable heterogeneity but are clearly different from L. rhamnosus GG at the genomic level.
28493885	13	45	theme	complement	1922:1931	arg1	system					1933:1938	the human complement system	1912:1938	the human complement system	1912:1938	All L. rhamnosus strains are still normally recognized by the human complement system.
28493885	11	46	theme	more	1569:1572	arg1	biofilms					1574:1581	more biofilms	1569:1581	more biofilms	1569:1581	Four out of 16 clinical blood isolates induced platelet aggregation and/or formed more biofilms than L. rhamnosus GG, which did not display platelet aggregation activity nor showed strong biofilm formation.
28493885	12	47	theme	genomic	1839:1845	arg1	level					1847:1851	the genomic level	1835:1851	the genomic level	1835:1851	These findings suggest that clinical L. rhamnosus isolates show considerable heterogeneity but are clearly different from L. rhamnosus GG at the genomic level.
28493885	9	48	theme	distinct	1367:1374	arg1	lineages					1376:1383	two distinct lineages	1363:1383	two distinct lineages	1363:1383	Genomic comparison between the clinical L. rhamnosus strains showed them to be clearly different from L. rhamnosus GG and to cluster in two distinct lineages.
28493885	1	49	from	composition	315:325	arg1	present					282:288	present	282:288	present	282:288	Lactobacillus rhamnosus strains are ubiquitous in fermented foods, and in the human body where they are commensals naturally present in the normal microbiota composition of gut, vagina and skin.
28493885	8	50	theme	isolated	1123:1130	arg1	strains					1132:1138	the isolated strains	1119:1138	the isolated strains	1119:1138	Furthermore, the isolated strains were evaluated for their ability to aggregate platelets and to form biofilms in vitro.
28493885	7	51	theme	radioligand	1086:1096	arg1	assays					1098:1103	radioligand assays	1086:1103	radioligand assays	1086:1103	Binding of complement regulators factor H and C4bp was also determined using radioligand assays.
28493885	0	52	dep	Lactobacillus	38:50	arg1	rhamnosus					52:60	Lactobacillus rhamnosus	38:60	Lactobacillus rhamnosus clinical isolates	38:78	Genotypic and phenotypic diversity of Lactobacillus rhamnosus clinical isolates, their comparison with strain GG and their recognition by complement system.
28493885	1	53	attach	present	282:288	arg2	commensals					261:270	commensals	261:270	commensals naturally present in the normal microbiota composition of gut, vagina and skin	261:349	Lactobacillus rhamnosus strains are ubiquitous in fermented foods, and in the human body where they are commensals naturally present in the normal microbiota composition of gut, vagina and skin.
28493885	1	53	attach	present	282:288	arg2	they					252:255	they	252:255	they	252:255	Lactobacillus rhamnosus strains are ubiquitous in fermented foods, and in the human body where they are commensals naturally present in the normal microbiota composition of gut, vagina and skin.
28493885	1	53	attach	present	282:288	arg1	composition					315:325	the normal microbiota composition	293:325	the normal microbiota composition of gut, vagina and skin	293:349	Lactobacillus rhamnosus strains are ubiquitous in fermented foods, and in the human body where they are commensals naturally present in the normal microbiota composition of gut, vagina and skin.
28493885	6	54	theme	serum	876:880	arg1	complement					882:891	serum complement	876:891	serum complement	876:891	The capacity of the L. rhamnosus strains to activate serum complement was studied using immunological assays for complement factor C3a and the terminal pathway complement complex (TCC).
28493885	11	55	theme	clinical	1502:1509	arg1	isolates					1517:1524	16 clinical blood isolates	1499:1524	16 clinical blood isolates	1499:1524	Four out of 16 clinical blood isolates induced platelet aggregation and/or formed more biofilms than L. rhamnosus GG, which did not display platelet aggregation activity nor showed strong biofilm formation.
28493885	12	56	theme	clinical	1722:1729	arg1	isolates					1744:1751	clinical L. rhamnosus isolates	1722:1751	clinical L. rhamnosus isolates	1722:1751	These findings suggest that clinical L. rhamnosus isolates show considerable heterogeneity but are clearly different from L. rhamnosus GG at the genomic level.
28493885	4	57	theme	strain	617:622	arg1	GG					624:625	the well-studied L. rhamnosus probiotic strain GG	577:625	the well-studied L. rhamnosus probiotic strain GG	577:625	The aim of the study was to examine the genomic and immunological properties of 16 clinical blood isolates of L. rhamnosus and to compare them to the well-studied L. rhamnosus probiotic strain GG.
28493885	11	58	theme	aggregation	1636:1646	arg1	activity					1648:1655	platelet aggregation activity	1627:1655	platelet aggregation activity	1627:1655	Four out of 16 clinical blood isolates induced platelet aggregation and/or formed more biofilms than L. rhamnosus GG, which did not display platelet aggregation activity nor showed strong biofilm formation.
28493885	10	59	theme	L.	1390:1391	arg1	strains					1403:1409	All L. rhamnosus strains	1386:1409	All L. rhamnosus strains	1386:1409	All L. rhamnosus strains activated complement in serum and none of them bound complement regulators.
28493885	11	60	theme	platelet	1534:1541	arg1	aggregation					1543:1553	platelet aggregation	1534:1553	platelet aggregation	1534:1553	Four out of 16 clinical blood isolates induced platelet aggregation and/or formed more biofilms than L. rhamnosus GG, which did not display platelet aggregation activity nor showed strong biofilm formation.
28493885	1	61	theme	fermented	207:215	arg1	foods					217:221	fermented foods	207:221	fermented foods	207:221	Lactobacillus rhamnosus strains are ubiquitous in fermented foods, and in the human body where they are commensals naturally present in the normal microbiota composition of gut, vagina and skin.
28493885	4	62	theme	well-studied	581:592	arg1	GG					624:625	the well-studied L. rhamnosus probiotic strain GG	577:625	the well-studied L. rhamnosus probiotic strain GG	577:625	The aim of the study was to examine the genomic and immunological properties of 16 clinical blood isolates of L. rhamnosus and to compare them to the well-studied L. rhamnosus probiotic strain GG.
28493885	7	63	theme	factor	1042:1047	arg1	H					1049:1049	factor H	1042:1049	factor H	1042:1049	Binding of complement regulators factor H and C4bp was also determined using radioligand assays.
28493885	7	63	theme	factor	1042:1047	arg1	regulators					1031:1040	complement regulators	1020:1040	complement regulators factor H and C4bp	1020:1058	Binding of complement regulators factor H and C4bp was also determined using radioligand assays.
28493885	5	64	from	laboratory	719:728	arg1	2005-2011					733:741	2005-2011	733:741	2005-2011	733:741	Blood cultures from bacteremic patients were collected at the Helsinki University Hospital laboratory in 2005-2011 and L. rhamnosus strains were isolated and characterized by genomic sequencing.
28493885	6	65	theme	complement	936:945	arg1	C3a					954:956	complement factor C3a	936:956	complement factor C3a	936:956	The capacity of the L. rhamnosus strains to activate serum complement was studied using immunological assays for complement factor C3a and the terminal pathway complement complex (TCC).
28493885	7	66	theme	complement	1020:1029	arg1	H					1049:1049	factor H	1042:1049	factor H	1042:1049	Binding of complement regulators factor H and C4bp was also determined using radioligand assays.
28493885	7	66	theme	complement	1020:1029	arg1	regulators					1031:1040	complement regulators	1020:1040	complement regulators factor H and C4bp	1020:1058	Binding of complement regulators factor H and C4bp was also determined using radioligand assays.
28493885	7	66	theme	complement	1020:1029	arg1	C4bp					1055:1058	C4bp	1055:1058	C4bp	1055:1058	Binding of complement regulators factor H and C4bp was also determined using radioligand assays.
28493885	9	67	theme	Genomic	1227:1233	arg1	comparison					1235:1244	Genomic comparison	1227:1244	Genomic comparison between the clinical L. rhamnosus strains	1227:1286	Genomic comparison between the clinical L. rhamnosus strains showed them to be clearly different from L. rhamnosus GG and to cluster in two distinct lineages.
28493885	5	68	theme	bacteremic	648:657	arg1	patients					659:666	bacteremic patients	648:666	bacteremic patients	648:666	Blood cultures from bacteremic patients were collected at the Helsinki University Hospital laboratory in 2005-2011 and L. rhamnosus strains were isolated and characterized by genomic sequencing.
28493885	1	69	from	foods	217:221	arg1	ubiquitous					193:202	ubiquitous	193:202	ubiquitous	193:202	Lactobacillus rhamnosus strains are ubiquitous in fermented foods, and in the human body where they are commensals naturally present in the normal microbiota composition of gut, vagina and skin.
28493885	0	70	with	recognition	123:133	arg1	strain					103:108	strain GG	103:111	strain GG	103:111	Genotypic and phenotypic diversity of Lactobacillus rhamnosus clinical isolates, their comparison with strain GG and their recognition by complement system.
28493885	9	71	theme	clinical	1258:1265	arg1	strains					1280:1286	the clinical L. rhamnosus strains	1254:1286	the clinical L. rhamnosus strains	1254:1286	Genomic comparison between the clinical L. rhamnosus strains showed them to be clearly different from L. rhamnosus GG and to cluster in two distinct lineages.
28493885	9	72	theme	rhamnosus	1270:1278	arg1	strains					1280:1286	the clinical L. rhamnosus strains	1254:1286	the clinical L. rhamnosus strains	1254:1286	Genomic comparison between the clinical L. rhamnosus strains showed them to be clearly different from L. rhamnosus GG and to cluster in two distinct lineages.
28493885	1	73	theme	Lactobacillus	157:169	arg1	strains					181:187	Lactobacillus rhamnosus strains	157:187	Lactobacillus rhamnosus strains	157:187	Lactobacillus rhamnosus strains are ubiquitous in fermented foods, and in the human body where they are commensals naturally present in the normal microbiota composition of gut, vagina and skin.
28493885	6	74	theme	pathway	975:981	arg1	TCC					1003:1005	TCC	1003:1005	TCC	1003:1005	The capacity of the L. rhamnosus strains to activate serum complement was studied using immunological assays for complement factor C3a and the terminal pathway complement complex (TCC).
28493885	6	74	theme	pathway	975:981	arg1	complex					994:1000	the terminal pathway complement complex	962:1000	the terminal pathway complement complex (TCC)	962:1006	The capacity of the L. rhamnosus strains to activate serum complement was studied using immunological assays for complement factor C3a and the terminal pathway complement complex (TCC).
28493885	4	75	theme	study	446:450	arg1	aim					435:437	The aim	431:437	The aim of the study	431:450	The aim of the study was to examine the genomic and immunological properties of 16 clinical blood isolates of L. rhamnosus and to compare them to the well-studied L. rhamnosus probiotic strain GG.
28493885	9	76	from	L.	1329:1330	arg1	different					1314:1322	different	1314:1322	different	1314:1322	Genomic comparison between the clinical L. rhamnosus strains showed them to be clearly different from L. rhamnosus GG and to cluster in two distinct lineages.
28493885	13	77	theme	L.	1858:1859	arg1	strains					1871:1877	All L. rhamnosus strains	1854:1877	All L. rhamnosus strains	1854:1877	All L. rhamnosus strains are still normally recognized by the human complement system.
28493885	0	78	theme	complement	138:147	arg1	system					149:154	complement system	138:154	complement system	138:154	Genotypic and phenotypic diversity of Lactobacillus rhamnosus clinical isolates, their comparison with strain GG and their recognition by complement system.
28493885	4	79	theme	blood	523:527	arg1	isolates					529:536	16 clinical blood isolates	511:536	16 clinical blood isolates of L. rhamnosus	511:552	The aim of the study was to examine the genomic and immunological properties of 16 clinical blood isolates of L. rhamnosus and to compare them to the well-studied L. rhamnosus probiotic strain GG.
28493885	1	80	theme	present	282:288	arg1	they					252:255	they	252:255	they	252:255	Lactobacillus rhamnosus strains are ubiquitous in fermented foods, and in the human body where they are commensals naturally present in the normal microbiota composition of gut, vagina and skin.
28493885	1	80	theme	present	282:288	arg1	commensals					261:270	commensals	261:270	commensals naturally present in the normal microbiota composition of gut, vagina and skin	261:349	Lactobacillus rhamnosus strains are ubiquitous in fermented foods, and in the human body where they are commensals naturally present in the normal microbiota composition of gut, vagina and skin.
28493885	10	81	theme	complement	1464:1473	arg1	regulators					1475:1484	bound complement regulators	1458:1484	bound complement regulators	1458:1484	All L. rhamnosus strains activated complement in serum and none of them bound complement regulators.
28493885	11	82	theme	biofilm	1675:1681	arg1	formation					1683:1691	strong biofilm formation	1668:1691	strong biofilm formation	1668:1691	Four out of 16 clinical blood isolates induced platelet aggregation and/or formed more biofilms than L. rhamnosus GG, which did not display platelet aggregation activity nor showed strong biofilm formation.
28493885	5	83	theme	rhamnosus	750:758	arg1	strains					760:766	L. rhamnosus strains	747:766	L. rhamnosus strains	747:766	Blood cultures from bacteremic patients were collected at the Helsinki University Hospital laboratory in 2005-2011 and L. rhamnosus strains were isolated and characterized by genomic sequencing.
28493885	6	84	theme	L.	843:844	arg1	strains					856:862	the L. rhamnosus strains	839:862	the L. rhamnosus strains	839:862	The capacity of the L. rhamnosus strains to activate serum complement was studied using immunological assays for complement factor C3a and the terminal pathway complement complex (TCC).
28493885	1	85	theme	human	235:239	arg1	body					241:244	the human body	231:244	the human body where they are commensals naturally present in the normal microbiota composition of gut, vagina and skin	231:349	Lactobacillus rhamnosus strains are ubiquitous in fermented foods, and in the human body where they are commensals naturally present in the normal microbiota composition of gut, vagina and skin.
28493885	0	86	with	diversity	25:33	arg1	strain					103:108	strain GG	103:111	strain GG	103:111	Genotypic and phenotypic diversity of Lactobacillus rhamnosus clinical isolates, their comparison with strain GG and their recognition by complement system.
28493885	0	87	theme	clinical	62:69	arg1	isolates					71:78	Lactobacillus rhamnosus clinical isolates	38:78	Lactobacillus rhamnosus clinical isolates	38:78	Genotypic and phenotypic diversity of Lactobacillus rhamnosus clinical isolates, their comparison with strain GG and their recognition by complement system.
28493885	1	88	theme	microbiota	304:313	arg1	composition					315:325	the normal microbiota composition	293:325	the normal microbiota composition of gut, vagina and skin	293:349	Lactobacillus rhamnosus strains are ubiquitous in fermented foods, and in the human body where they are commensals naturally present in the normal microbiota composition of gut, vagina and skin.
28493885	4	89	dep	L.	594:595	arg1	rhamnosus					597:605	rhamnosus	597:605	rhamnosus	597:605	The aim of the study was to examine the genomic and immunological properties of 16 clinical blood isolates of L. rhamnosus and to compare them to the well-studied L. rhamnosus probiotic strain GG.
28493885	8	90	dep	ability	1165:1171	arg1	aggregate					1176:1184	aggregate	1176:1184	aggregate	1176:1184	Furthermore, the isolated strains were evaluated for their ability to aggregate platelets and to form biofilms in vitro.
28493885	8	90	dep	ability	1165:1171	arg1	form					1203:1206	form	1203:1206	to form biofilms in vitro	1200:1224	Furthermore, the isolated strains were evaluated for their ability to aggregate platelets and to form biofilms in vitro.
28493885	5	91	from	patients	659:666	arg1	cultures					634:641	Blood cultures	628:641	Blood cultures from bacteremic patients	628:666	Blood cultures from bacteremic patients were collected at the Helsinki University Hospital laboratory in 2005-2011 and L. rhamnosus strains were isolated and characterized by genomic sequencing.
26120938	4	0	theme	biosynthetic	942:953	arg1	system					955:960	HS biosynthetic system	939:960	HS biosynthetic system	939:960	Real-time RT-PCR and Western-blot analyses revealed specific transcriptional patterns and expression levels of HS biosynthetic system both in different cell lines in vitro and cancers in vivo.
26120938	6	1	theme	human	1226:1230	arg1	fibroblasts					1232:1242	Normal human fibroblasts	1219:1242	Normal human fibroblasts	1219:1242	Normal human fibroblasts showed elongation-oriented HS biosynthesis, while PNT2 prostate epithelial cells had modification-oriented one.
26120938	8	2	theme	surface	1701:1707	arg1	pattern					1723:1729	like-to-normal cell surface glycosylation pattern	1681:1729	like-to-normal cell surface glycosylation pattern	1681:1729	Surprisingly, aggressive metastatic cancer cells (U2020, DU145, KRC/Y) retained modification-oriented HS biosynthesis similar to normal PNT2 cells, possibly enabling the cells to keep like-to-normal cell surface glycosylation pattern to escape antimetastatic control.
26120938	7	3	theme	biosynthetic	1476:1487	arg1	system					1489:1494	HS biosynthetic system	1473:1494	HS biosynthetic system	1473:1494	However, cancer epithelial cells demonstrated common tendency to acquire fibroblast-like elongation-oriented mode of HS biosynthetic system.
26120938	3	4	theme	cancer	692:697	arg1	lines					774:778	human normal (fibroblasts, PNT2) and cancer (MCF7, LNCaP, PC3, DU145, H157, H647, A549, U2020, U87, HT116, KRC/Y) cell lines	655:778	lines	774:778	To reveal cancer-related changes in transcriptional pattern of HS biosynthetic system, the expression of HS metabolism-involved genes (EXT1/2, NDST1/2, GLCE, 3OST1/HS3ST1, SULF1/2, HPSE) in human normal (fibroblasts, PNT2) and cancer (MCF7, LNCaP, PC3, DU145, H157, H647, A549, U2020, U87, HT116, KRC/Y) cell lines and breast, prostate, colon tumors was studied.
26120938	3	5	dep	normal	661:666	arg1	PNT2					682:685	PNT2	682:685	PNT2	682:685	To reveal cancer-related changes in transcriptional pattern of HS biosynthetic system, the expression of HS metabolism-involved genes (EXT1/2, NDST1/2, GLCE, 3OST1/HS3ST1, SULF1/2, HPSE) in human normal (fibroblasts, PNT2) and cancer (MCF7, LNCaP, PC3, DU145, H157, H647, A549, U2020, U87, HT116, KRC/Y) cell lines and breast, prostate, colon tumors was studied.
26120938	3	5	dep	normal	661:666	arg1	fibroblasts					669:679	fibroblasts	669:679	fibroblasts	669:679	To reveal cancer-related changes in transcriptional pattern of HS biosynthetic system, the expression of HS metabolism-involved genes (EXT1/2, NDST1/2, GLCE, 3OST1/HS3ST1, SULF1/2, HPSE) in human normal (fibroblasts, PNT2) and cancer (MCF7, LNCaP, PC3, DU145, H157, H647, A549, U2020, U87, HT116, KRC/Y) cell lines and breast, prostate, colon tumors was studied.
26120938	7	6	theme	common	1402:1407	arg1	tendency					1409:1416	common tendency	1402:1416	common tendency to acquire fibroblast-like elongation-oriented mode of HS biosynthetic system	1402:1494	However, cancer epithelial cells demonstrated common tendency to acquire fibroblast-like elongation-oriented mode of HS biosynthetic system.
26120938	9	7	theme	machinery	1841:1849	arg1	changes					1814:1820	the cell type-specific changes	1791:1820	the cell type-specific changes of HS-biosynthetic machinery in cancer cells	1791:1865	The obtained results show the cell type-specific changes of HS-biosynthetic machinery in cancer cells in vitro and tissue-specific changes in different cancers in vivo, supporting a close involvement of HS biosynthetic system in carcinogenesis.
26120938	9	7	theme	machinery	1841:1849	arg1	changes					1896:1902	tissue-specific changes	1880:1902	tissue-specific changes in different cancers	1880:1923	The obtained results show the cell type-specific changes of HS-biosynthetic machinery in cancer cells in vitro and tissue-specific changes in different cancers in vivo, supporting a close involvement of HS biosynthetic system in carcinogenesis.
26120938	3	8	dep	EXT1/2	600:605	arg1	GLCE					617:620	GLCE	617:620	GLCE	617:620	To reveal cancer-related changes in transcriptional pattern of HS biosynthetic system, the expression of HS metabolism-involved genes (EXT1/2, NDST1/2, GLCE, 3OST1/HS3ST1, SULF1/2, HPSE) in human normal (fibroblasts, PNT2) and cancer (MCF7, LNCaP, PC3, DU145, H157, H647, A549, U2020, U87, HT116, KRC/Y) cell lines and breast, prostate, colon tumors was studied.
26120938	3	8	dep	EXT1/2	600:605	arg1	HPSE					646:649	HPSE	646:649	HPSE	646:649	To reveal cancer-related changes in transcriptional pattern of HS biosynthetic system, the expression of HS metabolism-involved genes (EXT1/2, NDST1/2, GLCE, 3OST1/HS3ST1, SULF1/2, HPSE) in human normal (fibroblasts, PNT2) and cancer (MCF7, LNCaP, PC3, DU145, H157, H647, A549, U2020, U87, HT116, KRC/Y) cell lines and breast, prostate, colon tumors was studied.
26120938	3	8	dep	EXT1/2	600:605	arg1	NDST1/2					608:614	NDST1/2	608:614	NDST1/2	608:614	To reveal cancer-related changes in transcriptional pattern of HS biosynthetic system, the expression of HS metabolism-involved genes (EXT1/2, NDST1/2, GLCE, 3OST1/HS3ST1, SULF1/2, HPSE) in human normal (fibroblasts, PNT2) and cancer (MCF7, LNCaP, PC3, DU145, H157, H647, A549, U2020, U87, HT116, KRC/Y) cell lines and breast, prostate, colon tumors was studied.
26120938	3	8	dep	EXT1/2	600:605	arg1	SULF1/2					637:643	SULF1/2	637:643	SULF1/2	637:643	To reveal cancer-related changes in transcriptional pattern of HS biosynthetic system, the expression of HS metabolism-involved genes (EXT1/2, NDST1/2, GLCE, 3OST1/HS3ST1, SULF1/2, HPSE) in human normal (fibroblasts, PNT2) and cancer (MCF7, LNCaP, PC3, DU145, H157, H647, A549, U2020, U87, HT116, KRC/Y) cell lines and breast, prostate, colon tumors was studied.
26120938	3	8	dep	EXT1/2	600:605	arg1	3OST1/HS3ST1					623:634	3OST1/HS3ST1	623:634	3OST1/HS3ST1	623:634	To reveal cancer-related changes in transcriptional pattern of HS biosynthetic system, the expression of HS metabolism-involved genes (EXT1/2, NDST1/2, GLCE, 3OST1/HS3ST1, SULF1/2, HPSE) in human normal (fibroblasts, PNT2) and cancer (MCF7, LNCaP, PC3, DU145, H157, H647, A549, U2020, U87, HT116, KRC/Y) cell lines and breast, prostate, colon tumors was studied.
26120938	7	9	theme	elongation-oriented	1445:1463	arg1	mode					1465:1468	fibroblast-like elongation-oriented mode	1429:1468	fibroblast-like elongation-oriented mode of HS biosynthetic system	1429:1494	However, cancer epithelial cells demonstrated common tendency to acquire fibroblast-like elongation-oriented mode of HS biosynthetic system.
26120938	3	10	theme	genes	593:597	arg1	expression					556:565	the expression	552:565	the expression of HS metabolism-involved genes (EXT1/2, NDST1/2, GLCE, 3OST1/HS3ST1, SULF1/2, HPSE) in human normal (fibroblasts, PNT2) and cancer (MCF7, LNCaP, PC3, DU145, H157, H647, A549, U2020, U87, HT116, KRC/Y) cell lines and breast, prostate, colon tumors	552:813	To reveal cancer-related changes in transcriptional pattern of HS biosynthetic system, the expression of HS metabolism-involved genes (EXT1/2, NDST1/2, GLCE, 3OST1/HS3ST1, SULF1/2, HPSE) in human normal (fibroblasts, PNT2) and cancer (MCF7, LNCaP, PC3, DU145, H157, H647, A549, U2020, U87, HT116, KRC/Y) cell lines and breast, prostate, colon tumors was studied.
26120938	1	11	theme	HS	194:195	arg1	chains					197:202	their polysaccharide HS chains	173:202	their polysaccharide HS chains	173:202	Heparan sulfate (HS) proteoglycans are key components of cell microenvironment and fine structure of their polysaccharide HS chains plays an important role in cell-cell interactions, adhesion, migration and signaling.
26120938	7	12	theme	cancer	1365:1370	arg1	cells					1383:1387	cancer epithelial cells	1365:1387	cancer epithelial cells	1365:1387	However, cancer epithelial cells demonstrated common tendency to acquire fibroblast-like elongation-oriented mode of HS biosynthetic system.
26120938	9	13	theme	HS	1968:1969	arg1	system					1984:1989	HS biosynthetic system	1968:1989	HS biosynthetic system	1968:1989	The obtained results show the cell type-specific changes of HS-biosynthetic machinery in cancer cells in vitro and tissue-specific changes in different cancers in vivo, supporting a close involvement of HS biosynthetic system in carcinogenesis.
26120938	3	14	theme	MCF7	700:703	arg1	lines					774:778	human normal (fibroblasts, PNT2) and cancer (MCF7, LNCaP, PC3, DU145, H157, H647, A549, U2020, U87, HT116, KRC/Y) cell lines	655:778	lines	774:778	To reveal cancer-related changes in transcriptional pattern of HS biosynthetic system, the expression of HS metabolism-involved genes (EXT1/2, NDST1/2, GLCE, 3OST1/HS3ST1, SULF1/2, HPSE) in human normal (fibroblasts, PNT2) and cancer (MCF7, LNCaP, PC3, DU145, H157, H647, A549, U2020, U87, HT116, KRC/Y) cell lines and breast, prostate, colon tumors was studied.
26120938	6	15	theme	PNT2	1294:1297	arg1	cells					1319:1323	PNT2 prostate epithelial cells	1294:1323	PNT2 prostate epithelial cells	1294:1323	Normal human fibroblasts showed elongation-oriented HS biosynthesis, while PNT2 prostate epithelial cells had modification-oriented one.
26120938	3	16	theme	HS	570:571	arg1	genes					593:597	HS metabolism-involved genes	570:597	HS metabolism-involved genes (EXT1/2, NDST1/2, GLCE, 3OST1/HS3ST1, SULF1/2, HPSE)	570:650	To reveal cancer-related changes in transcriptional pattern of HS biosynthetic system, the expression of HS metabolism-involved genes (EXT1/2, NDST1/2, GLCE, 3OST1/HS3ST1, SULF1/2, HPSE) in human normal (fibroblasts, PNT2) and cancer (MCF7, LNCaP, PC3, DU145, H157, H647, A549, U2020, U87, HT116, KRC/Y) cell lines and breast, prostate, colon tumors was studied.
26120938	9	17	theme	system	1984:1989	arg1	involvement					1953:1963	a close involvement	1945:1963	a close involvement of HS biosynthetic system in carcinogenesis	1945:2007	The obtained results show the cell type-specific changes of HS-biosynthetic machinery in cancer cells in vitro and tissue-specific changes in different cancers in vivo, supporting a close involvement of HS biosynthetic system in carcinogenesis.
26120938	1	18	theme	important	213:221	arg1	role					223:226	an important role	210:226	an important role	210:226	Heparan sulfate (HS) proteoglycans are key components of cell microenvironment and fine structure of their polysaccharide HS chains plays an important role in cell-cell interactions, adhesion, migration and signaling.
26120938	6	19	theme	HS	1271:1272	arg1	biosynthesis					1274:1285	elongation-oriented HS biosynthesis	1251:1285	elongation-oriented HS biosynthesis	1251:1285	Normal human fibroblasts showed elongation-oriented HS biosynthesis, while PNT2 prostate epithelial cells had modification-oriented one.
26120938	1	20	theme	cell	129:132	arg1	microenvironment					134:149	cell microenvironment	129:149	cell microenvironment	129:149	Heparan sulfate (HS) proteoglycans are key components of cell microenvironment and fine structure of their polysaccharide HS chains plays an important role in cell-cell interactions, adhesion, migration and signaling.
26120938	4	21	theme	Western-blot	849:860	arg1	analyses					862:869	Western-blot analyses	849:869	Western-blot analyses	849:869	Real-time RT-PCR and Western-blot analyses revealed specific transcriptional patterns and expression levels of HS biosynthetic system both in different cell lines in vitro and cancers in vivo.
26120938	9	22	theme	cancer	1854:1859	arg1	cells					1861:1865	cancer cells	1854:1865	cancer cells	1854:1865	The obtained results show the cell type-specific changes of HS-biosynthetic machinery in cancer cells in vitro and tissue-specific changes in different cancers in vivo, supporting a close involvement of HS biosynthetic system in carcinogenesis.
26120938	3	23	theme	cell	769:772	arg1	lines					774:778	human normal (fibroblasts, PNT2) and cancer (MCF7, LNCaP, PC3, DU145, H157, H647, A549, U2020, U87, HT116, KRC/Y) cell lines	655:778	lines	774:778	To reveal cancer-related changes in transcriptional pattern of HS biosynthetic system, the expression of HS metabolism-involved genes (EXT1/2, NDST1/2, GLCE, 3OST1/HS3ST1, SULF1/2, HPSE) in human normal (fibroblasts, PNT2) and cancer (MCF7, LNCaP, PC3, DU145, H157, H647, A549, U2020, U87, HT116, KRC/Y) cell lines and breast, prostate, colon tumors was studied.
26120938	5	24	theme	biosynthetic	1178:1189	arg1	characterization					1201:1216	HS biosynthetic machinery characterization	1175:1216	HS biosynthetic machinery characterization	1175:1216	Balance between transcriptional activities of elongation- and post-synthetic modification- involved genes was suggested as most informative parameter for HS biosynthetic machinery characterization.
26120938	3	25	dep	genes	593:597	arg1	EXT1/2					600:605	EXT1/2	600:605	EXT1/2	600:605	To reveal cancer-related changes in transcriptional pattern of HS biosynthetic system, the expression of HS metabolism-involved genes (EXT1/2, NDST1/2, GLCE, 3OST1/HS3ST1, SULF1/2, HPSE) in human normal (fibroblasts, PNT2) and cancer (MCF7, LNCaP, PC3, DU145, H157, H647, A549, U2020, U87, HT116, KRC/Y) cell lines and breast, prostate, colon tumors was studied.
26120938	0	26	from	Tissue-specificity	0:17	arg1	cancer					64:69	cancer	64:69	cancer	64:69	Tissue-specificity of heparan sulfate biosynthetic machinery in cancer.
26120938	3	27	from	expression	556:565	arg1	prostate					792:799	prostate	792:799	prostate	792:799	To reveal cancer-related changes in transcriptional pattern of HS biosynthetic system, the expression of HS metabolism-involved genes (EXT1/2, NDST1/2, GLCE, 3OST1/HS3ST1, SULF1/2, HPSE) in human normal (fibroblasts, PNT2) and cancer (MCF7, LNCaP, PC3, DU145, H157, H647, A549, U2020, U87, HT116, KRC/Y) cell lines and breast, prostate, colon tumors was studied.
26120938	3	27	from	expression	556:565	arg1	lines					774:778	human normal (fibroblasts, PNT2) and cancer (MCF7, LNCaP, PC3, DU145, H157, H647, A549, U2020, U87, HT116, KRC/Y) cell lines	655:778	lines	774:778	To reveal cancer-related changes in transcriptional pattern of HS biosynthetic system, the expression of HS metabolism-involved genes (EXT1/2, NDST1/2, GLCE, 3OST1/HS3ST1, SULF1/2, HPSE) in human normal (fibroblasts, PNT2) and cancer (MCF7, LNCaP, PC3, DU145, H157, H647, A549, U2020, U87, HT116, KRC/Y) cell lines and breast, prostate, colon tumors was studied.
26120938	3	27	from	expression	556:565	arg1	tumors					808:813	colon tumors	802:813	colon tumors	802:813	To reveal cancer-related changes in transcriptional pattern of HS biosynthetic system, the expression of HS metabolism-involved genes (EXT1/2, NDST1/2, GLCE, 3OST1/HS3ST1, SULF1/2, HPSE) in human normal (fibroblasts, PNT2) and cancer (MCF7, LNCaP, PC3, DU145, H157, H647, A549, U2020, U87, HT116, KRC/Y) cell lines and breast, prostate, colon tumors was studied.
26120938	3	27	from	expression	556:565	arg1	breast					784:789	breast	784:789	breast	784:789	To reveal cancer-related changes in transcriptional pattern of HS biosynthetic system, the expression of HS metabolism-involved genes (EXT1/2, NDST1/2, GLCE, 3OST1/HS3ST1, SULF1/2, HPSE) in human normal (fibroblasts, PNT2) and cancer (MCF7, LNCaP, PC3, DU145, H157, H647, A549, U2020, U87, HT116, KRC/Y) cell lines and breast, prostate, colon tumors was studied.
26120938	3	27	from	expression	556:565	arg1	normal					661:666	normal	661:666	normal	661:666	To reveal cancer-related changes in transcriptional pattern of HS biosynthetic system, the expression of HS metabolism-involved genes (EXT1/2, NDST1/2, GLCE, 3OST1/HS3ST1, SULF1/2, HPSE) in human normal (fibroblasts, PNT2) and cancer (MCF7, LNCaP, PC3, DU145, H157, H647, A549, U2020, U87, HT116, KRC/Y) cell lines and breast, prostate, colon tumors was studied.
26120938	4	28	dep	system	955:960	arg1	both					962:965	both	962:965	both	962:965	Real-time RT-PCR and Western-blot analyses revealed specific transcriptional patterns and expression levels of HS biosynthetic system both in different cell lines in vitro and cancers in vivo.
26120938	1	29	theme	Heparan	72:78	arg1	sulfate					80:86	Heparan sulfate	72:86	Heparan sulfate (HS) proteoglycans	72:105	Heparan sulfate (HS) proteoglycans are key components of cell microenvironment and fine structure of their polysaccharide HS chains plays an important role in cell-cell interactions, adhesion, migration and signaling.
26120938	1	29	theme	Heparan	72:78	arg1	HS					89:90	HS	89:90	HS	89:90	Heparan sulfate (HS) proteoglycans are key components of cell microenvironment and fine structure of their polysaccharide HS chains plays an important role in cell-cell interactions, adhesion, migration and signaling.
26120938	8	30	theme	cancer	1533:1538	arg1	cells					1540:1544	aggressive metastatic cancer cells	1511:1544	aggressive metastatic cancer cells (U2020, DU145, KRC/Y)	1511:1566	Surprisingly, aggressive metastatic cancer cells (U2020, DU145, KRC/Y) retained modification-oriented HS biosynthesis similar to normal PNT2 cells, possibly enabling the cells to keep like-to-normal cell surface glycosylation pattern to escape antimetastatic control.
26120938	2	31	theme	machinery	383:391	arg1	structure					330:338	structure	330:338	structure	330:338	It is formed on non-template basis, so, structure and functional activity of HS biosynthetic machinery is crucial for correct HS biosynthesis and post-synthetic modification.
26120938	2	31	theme	machinery	383:391	arg1	activity					355:362	functional activity	344:362	functional activity	344:362	It is formed on non-template basis, so, structure and functional activity of HS biosynthetic machinery is crucial for correct HS biosynthesis and post-synthetic modification.
26120938	6	32	theme	epithelial	1308:1317	arg1	cells					1319:1323	PNT2 prostate epithelial cells	1294:1323	PNT2 prostate epithelial cells	1294:1323	Normal human fibroblasts showed elongation-oriented HS biosynthesis, while PNT2 prostate epithelial cells had modification-oriented one.
26120938	2	33	theme	post-synthetic	436:449	arg1	modification					451:462	post-synthetic modification	436:462	post-synthetic modification	436:462	It is formed on non-template basis, so, structure and functional activity of HS biosynthetic machinery is crucial for correct HS biosynthesis and post-synthetic modification.
26120938	9	34	theme	obtained	1769:1776	arg1	results					1778:1784	The obtained results	1765:1784	The obtained results	1765:1784	The obtained results show the cell type-specific changes of HS-biosynthetic machinery in cancer cells in vitro and tissue-specific changes in different cancers in vivo, supporting a close involvement of HS biosynthetic system in carcinogenesis.
26120938	2	35	theme	HS	367:368	arg1	machinery					383:391	HS biosynthetic machinery	367:391	HS biosynthetic machinery	367:391	It is formed on non-template basis, so, structure and functional activity of HS biosynthetic machinery is crucial for correct HS biosynthesis and post-synthetic modification.
26120938	4	36	theme	HS	939:940	arg1	system					955:960	HS biosynthetic system	939:960	HS biosynthetic system	939:960	Real-time RT-PCR and Western-blot analyses revealed specific transcriptional patterns and expression levels of HS biosynthetic system both in different cell lines in vitro and cancers in vivo.
26120938	2	37	theme	correct	408:414	arg1	biosynthesis					419:430	correct HS biosynthesis	408:430	correct HS biosynthesis	408:430	It is formed on non-template basis, so, structure and functional activity of HS biosynthetic machinery is crucial for correct HS biosynthesis and post-synthetic modification.
26120938	0	38	theme	heparan	22:28	arg1	machinery					51:59	heparan sulfate biosynthetic machinery	22:59	heparan sulfate biosynthetic machinery	22:59	Tissue-specificity of heparan sulfate biosynthetic machinery in cancer.
26120938	5	39	theme	modification-	1098:1110	arg1	genes					1121:1125	elongation- and post-synthetic modification- involved genes	1067:1125	elongation- and post-synthetic modification- involved genes	1067:1125	Balance between transcriptional activities of elongation- and post-synthetic modification- involved genes was suggested as most informative parameter for HS biosynthetic machinery characterization.
26120938	9	40	from	changes	1814:1820	arg1	cells					1861:1865	cancer cells	1854:1865	cancer cells	1854:1865	The obtained results show the cell type-specific changes of HS-biosynthetic machinery in cancer cells in vitro and tissue-specific changes in different cancers in vivo, supporting a close involvement of HS biosynthetic system in carcinogenesis.
26120938	9	40	from	changes	1814:1820	arg1	cancers					1917:1923	different cancers	1907:1923	different cancers	1907:1923	The obtained results show the cell type-specific changes of HS-biosynthetic machinery in cancer cells in vitro and tissue-specific changes in different cancers in vivo, supporting a close involvement of HS biosynthetic system in carcinogenesis.
26120938	4	41	theme	transcriptional	889:903	arg1	patterns					905:912	specific transcriptional patterns	880:912	specific transcriptional patterns	880:912	Real-time RT-PCR and Western-blot analyses revealed specific transcriptional patterns and expression levels of HS biosynthetic system both in different cell lines in vitro and cancers in vivo.
26120938	0	42	theme	biosynthetic	38:49	arg1	machinery					51:59	heparan sulfate biosynthetic machinery	22:59	heparan sulfate biosynthetic machinery	22:59	Tissue-specificity of heparan sulfate biosynthetic machinery in cancer.
26120938	5	43	theme	transcriptional	1037:1051	arg1	activities					1053:1062	transcriptional activities	1037:1062	transcriptional activities of elongation- and post-synthetic modification- involved genes	1037:1125	Balance between transcriptional activities of elongation- and post-synthetic modification- involved genes was suggested as most informative parameter for HS biosynthetic machinery characterization.
26120938	4	44	theme	cell	980:983	arg1	lines					985:989	different cell lines	970:989	different cell lines	970:989	Real-time RT-PCR and Western-blot analyses revealed specific transcriptional patterns and expression levels of HS biosynthetic system both in different cell lines in vitro and cancers in vivo.
26120938	8	45	theme	modification-oriented	1577:1597	arg1	biosynthesis					1602:1613	modification-oriented HS biosynthesis	1577:1613	modification-oriented HS biosynthesis similar to normal PNT2 cells	1577:1642	Surprisingly, aggressive metastatic cancer cells (U2020, DU145, KRC/Y) retained modification-oriented HS biosynthesis similar to normal PNT2 cells, possibly enabling the cells to keep like-to-normal cell surface glycosylation pattern to escape antimetastatic control.
26120938	3	46	theme	cancer-related	475:488	arg1	changes					490:496	cancer-related changes	475:496	cancer-related changes in transcriptional pattern of HS biosynthetic system	475:549	To reveal cancer-related changes in transcriptional pattern of HS biosynthetic system, the expression of HS metabolism-involved genes (EXT1/2, NDST1/2, GLCE, 3OST1/HS3ST1, SULF1/2, HPSE) in human normal (fibroblasts, PNT2) and cancer (MCF7, LNCaP, PC3, DU145, H157, H647, A549, U2020, U87, HT116, KRC/Y) cell lines and breast, prostate, colon tumors was studied.
26120938	3	47	theme	HS	528:529	arg1	system					544:549	HS biosynthetic system	528:549	HS biosynthetic system	528:549	To reveal cancer-related changes in transcriptional pattern of HS biosynthetic system, the expression of HS metabolism-involved genes (EXT1/2, NDST1/2, GLCE, 3OST1/HS3ST1, SULF1/2, HPSE) in human normal (fibroblasts, PNT2) and cancer (MCF7, LNCaP, PC3, DU145, H157, H647, A549, U2020, U87, HT116, KRC/Y) cell lines and breast, prostate, colon tumors was studied.
26120938	1	48	theme	cell-cell	231:239	arg1	adhesion					255:262	adhesion	255:262	adhesion	255:262	Heparan sulfate (HS) proteoglycans are key components of cell microenvironment and fine structure of their polysaccharide HS chains plays an important role in cell-cell interactions, adhesion, migration and signaling.
26120938	1	48	theme	cell-cell	231:239	arg1	interactions					241:252	cell-cell interactions	231:252	cell-cell interactions	231:252	Heparan sulfate (HS) proteoglycans are key components of cell microenvironment and fine structure of their polysaccharide HS chains plays an important role in cell-cell interactions, adhesion, migration and signaling.
26120938	1	48	theme	cell-cell	231:239	arg1	signaling					279:287	signaling	279:287	signaling	279:287	Heparan sulfate (HS) proteoglycans are key components of cell microenvironment and fine structure of their polysaccharide HS chains plays an important role in cell-cell interactions, adhesion, migration and signaling.
26120938	1	48	theme	cell-cell	231:239	arg1	migration					265:273	migration	265:273	migration	265:273	Heparan sulfate (HS) proteoglycans are key components of cell microenvironment and fine structure of their polysaccharide HS chains plays an important role in cell-cell interactions, adhesion, migration and signaling.
26120938	7	49	theme	system	1489:1494	arg1	mode					1465:1468	fibroblast-like elongation-oriented mode	1429:1468	fibroblast-like elongation-oriented mode of HS biosynthetic system	1429:1494	However, cancer epithelial cells demonstrated common tendency to acquire fibroblast-like elongation-oriented mode of HS biosynthetic system.
26120938	4	50	theme	system	955:960	arg1	patterns					905:912	specific transcriptional patterns	880:912	specific transcriptional patterns	880:912	Real-time RT-PCR and Western-blot analyses revealed specific transcriptional patterns and expression levels of HS biosynthetic system both in different cell lines in vitro and cancers in vivo.
26120938	4	50	theme	system	955:960	arg1	levels					929:934	expression levels	918:934	expression levels	918:934	Real-time RT-PCR and Western-blot analyses revealed specific transcriptional patterns and expression levels of HS biosynthetic system both in different cell lines in vitro and cancers in vivo.
26120938	4	50	theme	system	955:960	arg1	cancers					1004:1010	cancers	1004:1010	cancers	1004:1010	Real-time RT-PCR and Western-blot analyses revealed specific transcriptional patterns and expression levels of HS biosynthetic system both in different cell lines in vitro and cancers in vivo.
26120938	5	51	theme	genes	1121:1125	arg1	activities					1053:1062	transcriptional activities	1037:1062	transcriptional activities of elongation- and post-synthetic modification- involved genes	1037:1125	Balance between transcriptional activities of elongation- and post-synthetic modification- involved genes was suggested as most informative parameter for HS biosynthetic machinery characterization.
26120938	7	52	theme	HS	1473:1474	arg1	system					1489:1494	HS biosynthetic system	1473:1494	HS biosynthetic system	1473:1494	However, cancer epithelial cells demonstrated common tendency to acquire fibroblast-like elongation-oriented mode of HS biosynthetic system.
26120938	8	53	theme	PNT2	1633:1636	arg1	cells					1638:1642	normal PNT2 cells	1626:1642	normal PNT2 cells	1626:1642	Surprisingly, aggressive metastatic cancer cells (U2020, DU145, KRC/Y) retained modification-oriented HS biosynthesis similar to normal PNT2 cells, possibly enabling the cells to keep like-to-normal cell surface glycosylation pattern to escape antimetastatic control.
26120938	6	54	theme	Normal	1219:1224	arg1	fibroblasts					1232:1242	Normal human fibroblasts	1219:1242	Normal human fibroblasts	1219:1242	Normal human fibroblasts showed elongation-oriented HS biosynthesis, while PNT2 prostate epithelial cells had modification-oriented one.
26120938	8	55	theme	cell	1696:1699	arg1	pattern					1723:1729	like-to-normal cell surface glycosylation pattern	1681:1729	like-to-normal cell surface glycosylation pattern	1681:1729	Surprisingly, aggressive metastatic cancer cells (U2020, DU145, KRC/Y) retained modification-oriented HS biosynthesis similar to normal PNT2 cells, possibly enabling the cells to keep like-to-normal cell surface glycosylation pattern to escape antimetastatic control.
26120938	2	56	theme	non-template	306:317	arg1	basis					319:323	non-template basis	306:323	non-template basis	306:323	It is formed on non-template basis, so, structure and functional activity of HS biosynthetic machinery is crucial for correct HS biosynthesis and post-synthetic modification.
26120938	3	57	theme	system	544:549	arg1	pattern					517:523	transcriptional pattern	501:523	transcriptional pattern of HS biosynthetic system	501:549	To reveal cancer-related changes in transcriptional pattern of HS biosynthetic system, the expression of HS metabolism-involved genes (EXT1/2, NDST1/2, GLCE, 3OST1/HS3ST1, SULF1/2, HPSE) in human normal (fibroblasts, PNT2) and cancer (MCF7, LNCaP, PC3, DU145, H157, H647, A549, U2020, U87, HT116, KRC/Y) cell lines and breast, prostate, colon tumors was studied.
26120938	8	58	theme	glycosylation	1709:1721	arg1	pattern					1723:1729	like-to-normal cell surface glycosylation pattern	1681:1729	like-to-normal cell surface glycosylation pattern	1681:1729	Surprisingly, aggressive metastatic cancer cells (U2020, DU145, KRC/Y) retained modification-oriented HS biosynthesis similar to normal PNT2 cells, possibly enabling the cells to keep like-to-normal cell surface glycosylation pattern to escape antimetastatic control.
26120938	7	59	theme	fibroblast-like	1429:1443	arg1	mode					1465:1468	fibroblast-like elongation-oriented mode	1429:1468	fibroblast-like elongation-oriented mode of HS biosynthetic system	1429:1494	However, cancer epithelial cells demonstrated common tendency to acquire fibroblast-like elongation-oriented mode of HS biosynthetic system.
26120938	9	60	theme	close	1947:1951	arg1	involvement					1953:1963	a close involvement	1945:1963	a close involvement of HS biosynthetic system in carcinogenesis	1945:2007	The obtained results show the cell type-specific changes of HS-biosynthetic machinery in cancer cells in vitro and tissue-specific changes in different cancers in vivo, supporting a close involvement of HS biosynthetic system in carcinogenesis.
26120938	3	61	from	changes	490:496	arg1	pattern					517:523	transcriptional pattern	501:523	transcriptional pattern of HS biosynthetic system	501:549	To reveal cancer-related changes in transcriptional pattern of HS biosynthetic system, the expression of HS metabolism-involved genes (EXT1/2, NDST1/2, GLCE, 3OST1/HS3ST1, SULF1/2, HPSE) in human normal (fibroblasts, PNT2) and cancer (MCF7, LNCaP, PC3, DU145, H157, H647, A549, U2020, U87, HT116, KRC/Y) cell lines and breast, prostate, colon tumors was studied.
26120938	3	62	theme	metabolism-involved	573:591	arg1	genes					593:597	HS metabolism-involved genes	570:597	HS metabolism-involved genes (EXT1/2, NDST1/2, GLCE, 3OST1/HS3ST1, SULF1/2, HPSE)	570:650	To reveal cancer-related changes in transcriptional pattern of HS biosynthetic system, the expression of HS metabolism-involved genes (EXT1/2, NDST1/2, GLCE, 3OST1/HS3ST1, SULF1/2, HPSE) in human normal (fibroblasts, PNT2) and cancer (MCF7, LNCaP, PC3, DU145, H157, H647, A549, U2020, U87, HT116, KRC/Y) cell lines and breast, prostate, colon tumors was studied.
26120938	1	63	theme	polysaccharide	179:192	arg1	chains					197:202	their polysaccharide HS chains	173:202	their polysaccharide HS chains	173:202	Heparan sulfate (HS) proteoglycans are key components of cell microenvironment and fine structure of their polysaccharide HS chains plays an important role in cell-cell interactions, adhesion, migration and signaling.
26120938	6	64	contain	had	1325:1327	arg1	cells					1319:1323	PNT2 prostate epithelial cells	1294:1323	PNT2 prostate epithelial cells	1294:1323	Normal human fibroblasts showed elongation-oriented HS biosynthesis, while PNT2 prostate epithelial cells had modification-oriented one.
26120938	6	64	contain	had	1325:1327	arg2	one					1351:1353	modification-oriented one	1329:1353	modification-oriented one	1329:1353	Normal human fibroblasts showed elongation-oriented HS biosynthesis, while PNT2 prostate epithelial cells had modification-oriented one.
26120938	6	65	theme	prostate	1299:1306	arg1	cells					1319:1323	PNT2 prostate epithelial cells	1294:1323	PNT2 prostate epithelial cells	1294:1323	Normal human fibroblasts showed elongation-oriented HS biosynthesis, while PNT2 prostate epithelial cells had modification-oriented one.
26120938	8	66	theme	antimetastatic	1741:1754	arg1	control					1756:1762	antimetastatic control	1741:1762	antimetastatic control	1741:1762	Surprisingly, aggressive metastatic cancer cells (U2020, DU145, KRC/Y) retained modification-oriented HS biosynthesis similar to normal PNT2 cells, possibly enabling the cells to keep like-to-normal cell surface glycosylation pattern to escape antimetastatic control.
26120938	1	67	theme	chains	197:202	arg1	structure					160:168	fine structure	155:168	fine structure of their polysaccharide HS chains	155:202	Heparan sulfate (HS) proteoglycans are key components of cell microenvironment and fine structure of their polysaccharide HS chains plays an important role in cell-cell interactions, adhesion, migration and signaling.
26120938	7	68	theme	epithelial	1372:1381	arg1	cells					1383:1387	cancer epithelial cells	1365:1387	cancer epithelial cells	1365:1387	However, cancer epithelial cells demonstrated common tendency to acquire fibroblast-like elongation-oriented mode of HS biosynthetic system.
26120938	9	69	theme	biosynthetic	1971:1982	arg1	system					1984:1989	HS biosynthetic system	1968:1989	HS biosynthetic system	1968:1989	The obtained results show the cell type-specific changes of HS-biosynthetic machinery in cancer cells in vitro and tissue-specific changes in different cancers in vivo, supporting a close involvement of HS biosynthetic system in carcinogenesis.
26120938	9	70	theme	tissue-specific	1880:1894	arg1	changes					1896:1902	tissue-specific changes	1880:1902	tissue-specific changes in different cancers	1880:1923	The obtained results show the cell type-specific changes of HS-biosynthetic machinery in cancer cells in vitro and tissue-specific changes in different cancers in vivo, supporting a close involvement of HS biosynthetic system in carcinogenesis.
26120938	1	71	theme	key	111:113	arg1	proteoglycans					93:105	Heparan sulfate (HS) proteoglycans	72:105	Heparan sulfate (HS) proteoglycans	72:105	Heparan sulfate (HS) proteoglycans are key components of cell microenvironment and fine structure of their polysaccharide HS chains plays an important role in cell-cell interactions, adhesion, migration and signaling.
26120938	1	71	theme	key	111:113	arg1	components					115:124	key components	111:124	key components of cell microenvironment	111:149	Heparan sulfate (HS) proteoglycans are key components of cell microenvironment and fine structure of their polysaccharide HS chains plays an important role in cell-cell interactions, adhesion, migration and signaling.
26120938	8	72	theme	aggressive	1511:1520	arg1	cells					1540:1544	aggressive metastatic cancer cells	1511:1544	aggressive metastatic cancer cells (U2020, DU145, KRC/Y)	1511:1566	Surprisingly, aggressive metastatic cancer cells (U2020, DU145, KRC/Y) retained modification-oriented HS biosynthesis similar to normal PNT2 cells, possibly enabling the cells to keep like-to-normal cell surface glycosylation pattern to escape antimetastatic control.
26120938	6	73	theme	elongation-oriented	1251:1269	arg1	biosynthesis					1274:1285	elongation-oriented HS biosynthesis	1251:1285	elongation-oriented HS biosynthesis	1251:1285	Normal human fibroblasts showed elongation-oriented HS biosynthesis, while PNT2 prostate epithelial cells had modification-oriented one.
26120938	9	74	from	involvement	1953:1963	arg1	carcinogenesis					1994:2007	carcinogenesis	1994:2007	carcinogenesis	1994:2007	The obtained results show the cell type-specific changes of HS-biosynthetic machinery in cancer cells in vitro and tissue-specific changes in different cancers in vivo, supporting a close involvement of HS biosynthetic system in carcinogenesis.
26120938	1	75	theme	microenvironment	134:149	arg1	proteoglycans					93:105	Heparan sulfate (HS) proteoglycans	72:105	Heparan sulfate (HS) proteoglycans	72:105	Heparan sulfate (HS) proteoglycans are key components of cell microenvironment and fine structure of their polysaccharide HS chains plays an important role in cell-cell interactions, adhesion, migration and signaling.
26120938	1	75	theme	microenvironment	134:149	arg1	components					115:124	key components	111:124	key components of cell microenvironment	111:149	Heparan sulfate (HS) proteoglycans are key components of cell microenvironment and fine structure of their polysaccharide HS chains plays an important role in cell-cell interactions, adhesion, migration and signaling.
26120938	5	76	theme	HS	1175:1176	arg1	characterization					1201:1216	HS biosynthetic machinery characterization	1175:1216	HS biosynthetic machinery characterization	1175:1216	Balance between transcriptional activities of elongation- and post-synthetic modification- involved genes was suggested as most informative parameter for HS biosynthetic machinery characterization.
26120938	1	77	theme	fine	155:158	arg1	structure					160:168	fine structure	155:168	fine structure of their polysaccharide HS chains	155:202	Heparan sulfate (HS) proteoglycans are key components of cell microenvironment and fine structure of their polysaccharide HS chains plays an important role in cell-cell interactions, adhesion, migration and signaling.
26120938	9	78	theme	HS-biosynthetic	1825:1839	arg1	machinery					1841:1849	HS-biosynthetic machinery	1825:1849	HS-biosynthetic machinery	1825:1849	The obtained results show the cell type-specific changes of HS-biosynthetic machinery in cancer cells in vitro and tissue-specific changes in different cancers in vivo, supporting a close involvement of HS biosynthetic system in carcinogenesis.
26120938	8	79	theme	metastatic	1522:1531	arg1	cells					1540:1544	aggressive metastatic cancer cells	1511:1544	aggressive metastatic cancer cells (U2020, DU145, KRC/Y)	1511:1566	Surprisingly, aggressive metastatic cancer cells (U2020, DU145, KRC/Y) retained modification-oriented HS biosynthesis similar to normal PNT2 cells, possibly enabling the cells to keep like-to-normal cell surface glycosylation pattern to escape antimetastatic control.
26120938	5	80	theme	machinery	1191:1199	arg1	characterization					1201:1216	HS biosynthetic machinery characterization	1175:1216	HS biosynthetic machinery characterization	1175:1216	Balance between transcriptional activities of elongation- and post-synthetic modification- involved genes was suggested as most informative parameter for HS biosynthetic machinery characterization.
26120938	6	81	theme	modification-oriented	1329:1349	arg1	one					1351:1353	modification-oriented one	1329:1353	modification-oriented one	1329:1353	Normal human fibroblasts showed elongation-oriented HS biosynthesis, while PNT2 prostate epithelial cells had modification-oriented one.
26120938	9	82	from	changes	1896:1902	arg1	cells					1861:1865	cancer cells	1854:1865	cancer cells	1854:1865	The obtained results show the cell type-specific changes of HS-biosynthetic machinery in cancer cells in vitro and tissue-specific changes in different cancers in vivo, supporting a close involvement of HS biosynthetic system in carcinogenesis.
26120938	9	82	from	changes	1896:1902	arg1	cancers					1917:1923	different cancers	1907:1923	different cancers	1907:1923	The obtained results show the cell type-specific changes of HS-biosynthetic machinery in cancer cells in vitro and tissue-specific changes in different cancers in vivo, supporting a close involvement of HS biosynthetic system in carcinogenesis.
26120938	9	83	theme	type-specific	1800:1812	arg1	changes					1814:1820	the cell type-specific changes	1791:1820	the cell type-specific changes of HS-biosynthetic machinery in cancer cells	1791:1865	The obtained results show the cell type-specific changes of HS-biosynthetic machinery in cancer cells in vitro and tissue-specific changes in different cancers in vivo, supporting a close involvement of HS biosynthetic system in carcinogenesis.
26120938	1	84	theme	sulfate	80:86	arg1	proteoglycans					93:105	Heparan sulfate (HS) proteoglycans	72:105	Heparan sulfate (HS) proteoglycans	72:105	Heparan sulfate (HS) proteoglycans are key components of cell microenvironment and fine structure of their polysaccharide HS chains plays an important role in cell-cell interactions, adhesion, migration and signaling.
26120938	1	84	theme	sulfate	80:86	arg1	components					115:124	key components	111:124	key components of cell microenvironment	111:149	Heparan sulfate (HS) proteoglycans are key components of cell microenvironment and fine structure of their polysaccharide HS chains plays an important role in cell-cell interactions, adhesion, migration and signaling.
26120938	4	85	theme	Real-time	828:836	arg1	RT-PCR					838:843	Real-time RT-PCR	828:843	Real-time RT-PCR	828:843	Real-time RT-PCR and Western-blot analyses revealed specific transcriptional patterns and expression levels of HS biosynthetic system both in different cell lines in vitro and cancers in vivo.
26120938	2	86	theme	biosynthetic	370:381	arg1	machinery					383:391	HS biosynthetic machinery	367:391	HS biosynthetic machinery	367:391	It is formed on non-template basis, so, structure and functional activity of HS biosynthetic machinery is crucial for correct HS biosynthesis and post-synthetic modification.
26120938	4	87	theme	expression	918:927	arg1	levels					929:934	expression levels	918:934	expression levels	918:934	Real-time RT-PCR and Western-blot analyses revealed specific transcriptional patterns and expression levels of HS biosynthetic system both in different cell lines in vitro and cancers in vivo.
26120938	0	88	theme	sulfate	30:36	arg1	machinery					51:59	heparan sulfate biosynthetic machinery	22:59	heparan sulfate biosynthetic machinery	22:59	Tissue-specificity of heparan sulfate biosynthetic machinery in cancer.
26120938	5	89	theme	post-synthetic	1083:1096	arg1	genes					1121:1125	elongation- and post-synthetic modification- involved genes	1067:1125	elongation- and post-synthetic modification- involved genes	1067:1125	Balance between transcriptional activities of elongation- and post-synthetic modification- involved genes was suggested as most informative parameter for HS biosynthetic machinery characterization.
26120938	3	90	dep	lines	774:778	arg1	H647					731:734	human normal (fibroblasts, PNT2) and cancer (MCF7, LNCaP, PC3, DU145, H157, H647, A549, U2020, U87, HT116, KRC/Y) cell lines	655:778	H647	731:734	To reveal cancer-related changes in transcriptional pattern of HS biosynthetic system, the expression of HS metabolism-involved genes (EXT1/2, NDST1/2, GLCE, 3OST1/HS3ST1, SULF1/2, HPSE) in human normal (fibroblasts, PNT2) and cancer (MCF7, LNCaP, PC3, DU145, H157, H647, A549, U2020, U87, HT116, KRC/Y) cell lines and breast, prostate, colon tumors was studied.
26120938	3	90	dep	lines	774:778	arg1	U87					750:752	human normal (fibroblasts, PNT2) and cancer (MCF7, LNCaP, PC3, DU145, H157, H647, A549, U2020, U87, HT116, KRC/Y) cell lines	655:778	U87	750:752	To reveal cancer-related changes in transcriptional pattern of HS biosynthetic system, the expression of HS metabolism-involved genes (EXT1/2, NDST1/2, GLCE, 3OST1/HS3ST1, SULF1/2, HPSE) in human normal (fibroblasts, PNT2) and cancer (MCF7, LNCaP, PC3, DU145, H157, H647, A549, U2020, U87, HT116, KRC/Y) cell lines and breast, prostate, colon tumors was studied.
26120938	3	90	dep	lines	774:778	arg1	PC3					713:715	human normal (fibroblasts, PNT2) and cancer (MCF7, LNCaP, PC3, DU145, H157, H647, A549, U2020, U87, HT116, KRC/Y) cell lines	655:778	PC3	713:715	To reveal cancer-related changes in transcriptional pattern of HS biosynthetic system, the expression of HS metabolism-involved genes (EXT1/2, NDST1/2, GLCE, 3OST1/HS3ST1, SULF1/2, HPSE) in human normal (fibroblasts, PNT2) and cancer (MCF7, LNCaP, PC3, DU145, H157, H647, A549, U2020, U87, HT116, KRC/Y) cell lines and breast, prostate, colon tumors was studied.
26120938	3	90	dep	lines	774:778	arg1	HT116					755:759	human normal (fibroblasts, PNT2) and cancer (MCF7, LNCaP, PC3, DU145, H157, H647, A549, U2020, U87, HT116, KRC/Y) cell lines	655:778	HT116	755:759	To reveal cancer-related changes in transcriptional pattern of HS biosynthetic system, the expression of HS metabolism-involved genes (EXT1/2, NDST1/2, GLCE, 3OST1/HS3ST1, SULF1/2, HPSE) in human normal (fibroblasts, PNT2) and cancer (MCF7, LNCaP, PC3, DU145, H157, H647, A549, U2020, U87, HT116, KRC/Y) cell lines and breast, prostate, colon tumors was studied.
26120938	3	90	dep	lines	774:778	arg1	DU145					718:722	human normal (fibroblasts, PNT2) and cancer (MCF7, LNCaP, PC3, DU145, H157, H647, A549, U2020, U87, HT116, KRC/Y) cell lines	655:778	DU145	718:722	To reveal cancer-related changes in transcriptional pattern of HS biosynthetic system, the expression of HS metabolism-involved genes (EXT1/2, NDST1/2, GLCE, 3OST1/HS3ST1, SULF1/2, HPSE) in human normal (fibroblasts, PNT2) and cancer (MCF7, LNCaP, PC3, DU145, H157, H647, A549, U2020, U87, HT116, KRC/Y) cell lines and breast, prostate, colon tumors was studied.
26120938	3	90	dep	lines	774:778	arg1	A549					737:740	human normal (fibroblasts, PNT2) and cancer (MCF7, LNCaP, PC3, DU145, H157, H647, A549, U2020, U87, HT116, KRC/Y) cell lines	655:778	A549	737:740	To reveal cancer-related changes in transcriptional pattern of HS biosynthetic system, the expression of HS metabolism-involved genes (EXT1/2, NDST1/2, GLCE, 3OST1/HS3ST1, SULF1/2, HPSE) in human normal (fibroblasts, PNT2) and cancer (MCF7, LNCaP, PC3, DU145, H157, H647, A549, U2020, U87, HT116, KRC/Y) cell lines and breast, prostate, colon tumors was studied.
26120938	3	90	dep	lines	774:778	arg1	U2020					743:747	human normal (fibroblasts, PNT2) and cancer (MCF7, LNCaP, PC3, DU145, H157, H647, A549, U2020, U87, HT116, KRC/Y) cell lines	655:778	U2020	743:747	To reveal cancer-related changes in transcriptional pattern of HS biosynthetic system, the expression of HS metabolism-involved genes (EXT1/2, NDST1/2, GLCE, 3OST1/HS3ST1, SULF1/2, HPSE) in human normal (fibroblasts, PNT2) and cancer (MCF7, LNCaP, PC3, DU145, H157, H647, A549, U2020, U87, HT116, KRC/Y) cell lines and breast, prostate, colon tumors was studied.
26120938	3	90	dep	lines	774:778	arg1	KRC/Y					762:766	human normal (fibroblasts, PNT2) and cancer (MCF7, LNCaP, PC3, DU145, H157, H647, A549, U2020, U87, HT116, KRC/Y) cell lines	655:778	KRC/Y	762:766	To reveal cancer-related changes in transcriptional pattern of HS biosynthetic system, the expression of HS metabolism-involved genes (EXT1/2, NDST1/2, GLCE, 3OST1/HS3ST1, SULF1/2, HPSE) in human normal (fibroblasts, PNT2) and cancer (MCF7, LNCaP, PC3, DU145, H157, H647, A549, U2020, U87, HT116, KRC/Y) cell lines and breast, prostate, colon tumors was studied.
26120938	3	90	dep	lines	774:778	arg1	LNCaP					706:710	human normal (fibroblasts, PNT2) and cancer (MCF7, LNCaP, PC3, DU145, H157, H647, A549, U2020, U87, HT116, KRC/Y) cell lines	655:778	LNCaP	706:710	To reveal cancer-related changes in transcriptional pattern of HS biosynthetic system, the expression of HS metabolism-involved genes (EXT1/2, NDST1/2, GLCE, 3OST1/HS3ST1, SULF1/2, HPSE) in human normal (fibroblasts, PNT2) and cancer (MCF7, LNCaP, PC3, DU145, H157, H647, A549, U2020, U87, HT116, KRC/Y) cell lines and breast, prostate, colon tumors was studied.
26120938	3	90	dep	lines	774:778	arg1	H157					725:728	human normal (fibroblasts, PNT2) and cancer (MCF7, LNCaP, PC3, DU145, H157, H647, A549, U2020, U87, HT116, KRC/Y) cell lines	655:778	H157	725:728	To reveal cancer-related changes in transcriptional pattern of HS biosynthetic system, the expression of HS metabolism-involved genes (EXT1/2, NDST1/2, GLCE, 3OST1/HS3ST1, SULF1/2, HPSE) in human normal (fibroblasts, PNT2) and cancer (MCF7, LNCaP, PC3, DU145, H157, H647, A549, U2020, U87, HT116, KRC/Y) cell lines and breast, prostate, colon tumors was studied.
26120938	2	91	theme	HS	416:417	arg1	biosynthesis					419:430	correct HS biosynthesis	408:430	correct HS biosynthesis	408:430	It is formed on non-template basis, so, structure and functional activity of HS biosynthetic machinery is crucial for correct HS biosynthesis and post-synthetic modification.
26120938	0	92	theme	machinery	51:59	arg1	Tissue-specificity					0:17	Tissue-specificity	0:17	Tissue-specificity of heparan sulfate biosynthetic machinery in cancer.	0:70	Tissue-specificity of heparan sulfate biosynthetic machinery in cancer.
26120938	5	93	theme	involved	1112:1119	arg1	genes					1121:1125	elongation- and post-synthetic modification- involved genes	1067:1125	elongation- and post-synthetic modification- involved genes	1067:1125	Balance between transcriptional activities of elongation- and post-synthetic modification- involved genes was suggested as most informative parameter for HS biosynthetic machinery characterization.
26120938	3	94	theme	colon	802:806	arg1	tumors					808:813	colon tumors	802:813	colon tumors	802:813	To reveal cancer-related changes in transcriptional pattern of HS biosynthetic system, the expression of HS metabolism-involved genes (EXT1/2, NDST1/2, GLCE, 3OST1/HS3ST1, SULF1/2, HPSE) in human normal (fibroblasts, PNT2) and cancer (MCF7, LNCaP, PC3, DU145, H157, H647, A549, U2020, U87, HT116, KRC/Y) cell lines and breast, prostate, colon tumors was studied.
26120938	3	94	theme	colon	802:806	arg1	breast					784:789	breast	784:789	breast	784:789	To reveal cancer-related changes in transcriptional pattern of HS biosynthetic system, the expression of HS metabolism-involved genes (EXT1/2, NDST1/2, GLCE, 3OST1/HS3ST1, SULF1/2, HPSE) in human normal (fibroblasts, PNT2) and cancer (MCF7, LNCaP, PC3, DU145, H157, H647, A549, U2020, U87, HT116, KRC/Y) cell lines and breast, prostate, colon tumors was studied.
26120938	4	95	theme	specific	880:887	arg1	patterns					905:912	specific transcriptional patterns	880:912	specific transcriptional patterns	880:912	Real-time RT-PCR and Western-blot analyses revealed specific transcriptional patterns and expression levels of HS biosynthetic system both in different cell lines in vitro and cancers in vivo.
26120938	8	96	dep	cells	1540:1544	arg1	KRC/Y					1561:1565	KRC/Y	1561:1565	KRC/Y	1561:1565	Surprisingly, aggressive metastatic cancer cells (U2020, DU145, KRC/Y) retained modification-oriented HS biosynthesis similar to normal PNT2 cells, possibly enabling the cells to keep like-to-normal cell surface glycosylation pattern to escape antimetastatic control.
26120938	8	96	dep	cells	1540:1544	arg1	DU145					1554:1558	DU145	1554:1558	DU145	1554:1558	Surprisingly, aggressive metastatic cancer cells (U2020, DU145, KRC/Y) retained modification-oriented HS biosynthesis similar to normal PNT2 cells, possibly enabling the cells to keep like-to-normal cell surface glycosylation pattern to escape antimetastatic control.
26120938	8	96	dep	cells	1540:1544	arg1	U2020					1547:1551	U2020	1547:1551	U2020	1547:1551	Surprisingly, aggressive metastatic cancer cells (U2020, DU145, KRC/Y) retained modification-oriented HS biosynthesis similar to normal PNT2 cells, possibly enabling the cells to keep like-to-normal cell surface glycosylation pattern to escape antimetastatic control.
26120938	8	97	theme	HS	1599:1600	arg1	biosynthesis					1602:1613	modification-oriented HS biosynthesis	1577:1613	modification-oriented HS biosynthesis similar to normal PNT2 cells	1577:1642	Surprisingly, aggressive metastatic cancer cells (U2020, DU145, KRC/Y) retained modification-oriented HS biosynthesis similar to normal PNT2 cells, possibly enabling the cells to keep like-to-normal cell surface glycosylation pattern to escape antimetastatic control.
26120938	3	98	theme	transcriptional	501:515	arg1	pattern					517:523	transcriptional pattern	501:523	transcriptional pattern of HS biosynthetic system	501:549	To reveal cancer-related changes in transcriptional pattern of HS biosynthetic system, the expression of HS metabolism-involved genes (EXT1/2, NDST1/2, GLCE, 3OST1/HS3ST1, SULF1/2, HPSE) in human normal (fibroblasts, PNT2) and cancer (MCF7, LNCaP, PC3, DU145, H157, H647, A549, U2020, U87, HT116, KRC/Y) cell lines and breast, prostate, colon tumors was studied.
26120938	5	99	theme	elongation-	1067:1077	arg1	genes					1121:1125	elongation- and post-synthetic modification- involved genes	1067:1125	elongation- and post-synthetic modification- involved genes	1067:1125	Balance between transcriptional activities of elongation- and post-synthetic modification- involved genes was suggested as most informative parameter for HS biosynthetic machinery characterization.
26120938	4	100	theme	different	970:978	arg1	lines					985:989	different cell lines	970:989	different cell lines	970:989	Real-time RT-PCR and Western-blot analyses revealed specific transcriptional patterns and expression levels of HS biosynthetic system both in different cell lines in vitro and cancers in vivo.
26120938	8	101	theme	similar	1615:1621	arg1	biosynthesis					1602:1613	modification-oriented HS biosynthesis	1577:1613	modification-oriented HS biosynthesis similar to normal PNT2 cells	1577:1642	Surprisingly, aggressive metastatic cancer cells (U2020, DU145, KRC/Y) retained modification-oriented HS biosynthesis similar to normal PNT2 cells, possibly enabling the cells to keep like-to-normal cell surface glycosylation pattern to escape antimetastatic control.
26120938	2	102	theme	functional	344:353	arg1	activity					355:362	functional activity	344:362	functional activity	344:362	It is formed on non-template basis, so, structure and functional activity of HS biosynthetic machinery is crucial for correct HS biosynthesis and post-synthetic modification.
26120938	5	103	theme	informative	1149:1159	arg1	Balance					1021:1027	Balance	1021:1027	Balance between transcriptional activities of elongation- and post-synthetic modification- involved genes	1021:1125	Balance between transcriptional activities of elongation- and post-synthetic modification- involved genes was suggested as most informative parameter for HS biosynthetic machinery characterization.
26120938	5	103	theme	informative	1149:1159	arg1	parameter					1161:1169	most informative parameter	1144:1169	most informative parameter for HS biosynthetic machinery characterization	1144:1216	Balance between transcriptional activities of elongation- and post-synthetic modification- involved genes was suggested as most informative parameter for HS biosynthetic machinery characterization.
26120938	9	104	theme	different	1907:1915	arg1	cancers					1917:1923	different cancers	1907:1923	different cancers	1907:1923	The obtained results show the cell type-specific changes of HS-biosynthetic machinery in cancer cells in vitro and tissue-specific changes in different cancers in vivo, supporting a close involvement of HS biosynthetic system in carcinogenesis.
26120938	8	105	theme	normal	1626:1631	arg1	cells					1638:1642	normal PNT2 cells	1626:1642	normal PNT2 cells	1626:1642	Surprisingly, aggressive metastatic cancer cells (U2020, DU145, KRC/Y) retained modification-oriented HS biosynthesis similar to normal PNT2 cells, possibly enabling the cells to keep like-to-normal cell surface glycosylation pattern to escape antimetastatic control.
26120938	3	106	theme	biosynthetic	531:542	arg1	system					544:549	HS biosynthetic system	528:549	HS biosynthetic system	528:549	To reveal cancer-related changes in transcriptional pattern of HS biosynthetic system, the expression of HS metabolism-involved genes (EXT1/2, NDST1/2, GLCE, 3OST1/HS3ST1, SULF1/2, HPSE) in human normal (fibroblasts, PNT2) and cancer (MCF7, LNCaP, PC3, DU145, H157, H647, A549, U2020, U87, HT116, KRC/Y) cell lines and breast, prostate, colon tumors was studied.
26120938	8	107	theme	like-to-normal	1681:1694	arg1	pattern					1723:1729	like-to-normal cell surface glycosylation pattern	1681:1729	like-to-normal cell surface glycosylation pattern	1681:1729	Surprisingly, aggressive metastatic cancer cells (U2020, DU145, KRC/Y) retained modification-oriented HS biosynthesis similar to normal PNT2 cells, possibly enabling the cells to keep like-to-normal cell surface glycosylation pattern to escape antimetastatic control.
25158570	7	0	theme	medium	1347:1352	arg1	hardness					1359:1366	medium core hardness	1347:1366	medium core hardness	1347:1366	The results also show that the adhesion force, regardless of the tablet core formulation, is highest at medium core hardness.
25158570	4	1	theme	microcrystalline	657:672	arg1	cellulose					674:682	microcrystalline cellulose	657:682	microcrystalline cellulose	657:682	Tablet cores composed of microcrystalline cellulose and lactose with and without Mg stearate and compressed at three different compression forces were tested for wettability with coating formulations containing PEG 400 and polysorbate 80.
25158570	1	2	theme	different	154:162	arg1	additives					164:172	different additives	154:172	different additives	154:172	The influence of different additives on the mechanical properties of hydroxypropyl methylcellulose (HPMC) free films was studied using tensile testing.
25158570	0	3	theme	mechanical	62:71	arg1	properties					86:95	the mechanical and adhesion properties	58:95	properties	86:95	The effect of cores and coating dispersion composition on the mechanical and adhesion properties of hydroxypropyl methylcellulose films.
25158570	0	4	from	effect	4:9	arg1	properties					86:95	the mechanical and adhesion properties	58:95	properties	86:95	The effect of cores and coating dispersion composition on the mechanical and adhesion properties of hydroxypropyl methylcellulose films.
25158570	1	5	theme	additives	164:172	arg1	influence					141:149	The influence	137:149	The influence of different additives on the mechanical properties of hydroxypropyl methylcellulose (HPMC) free films	137:252	The influence of different additives on the mechanical properties of hydroxypropyl methylcellulose (HPMC) free films was studied using tensile testing.
25158570	6	6	from	influence	1149:1157	arg1	force					1175:1179	the adhesion force	1162:1179	the adhesion force	1162:1179	The addition of Mg stearate in the core led to reduced adhesion of the film coating with PEG 400, whereas the influence on the adhesion force of the film coating containing polysorbate 80 was negligible.
25158570	5	7	theme	core	959:962	arg1	hardness					964:971	core hardness	959:971	core hardness	959:971	For formulations with no Mg stearate added, the contact angle decreased with increasing core hardness and it also coincided with greater adhesion force of the coating.
25158570	6	8	theme	stearate	1058:1065	arg1	addition					1043:1050	The addition	1039:1050	The addition of Mg stearate in the core	1039:1077	The addition of Mg stearate in the core led to reduced adhesion of the film coating with PEG 400, whereas the influence on the adhesion force of the film coating containing polysorbate 80 was negligible.
25158570	7	9	theme	core	1354:1357	arg1	hardness					1359:1366	medium core hardness	1347:1366	medium core hardness	1347:1366	The results also show that the adhesion force, regardless of the tablet core formulation, is highest at medium core hardness.
25158570	3	10	theme	most	536:539	arg1	influence					541:549	the most influence	532:549	the most influence on the films' mechanical properties	532:585	The results showed that the addition of PEG 400 and polysorbate 80 into the coating formulation had the most influence on the films' mechanical properties compared to the HPMC film used as a control.
25158570	0	11	theme	adhesion	77:84	arg1	properties					86:95	the mechanical and adhesion properties	58:95	properties	86:95	The effect of cores and coating dispersion composition on the mechanical and adhesion properties of hydroxypropyl methylcellulose films.
25158570	1	12	from	influence	141:149	arg1	properties					192:201	the mechanical properties	177:201	the mechanical properties of hydroxypropyl methylcellulose (HPMC) free films	177:252	The influence of different additives on the mechanical properties of hydroxypropyl methylcellulose (HPMC) free films was studied using tensile testing.
25158570	2	13	theme	Free	289:292	arg1	films					294:298	Free films	289:298	Free films	289:298	Free films were prepared using the cast method and sliced into bands, and their tensile strength and maximal elongation at break was measured.
25158570	6	14	contain	containing	1201:1210	arg2	polysorbate					1212:1222	polysorbate 80	1212:1225	polysorbate 80	1212:1225	The addition of Mg stearate in the core led to reduced adhesion of the film coating with PEG 400, whereas the influence on the adhesion force of the film coating containing polysorbate 80 was negligible.
25158570	6	14	contain	containing	1201:1210	arg1	coating					1193:1199	the film coating	1184:1199	the film coating containing polysorbate 80	1184:1225	The addition of Mg stearate in the core led to reduced adhesion of the film coating with PEG 400, whereas the influence on the adhesion force of the film coating containing polysorbate 80 was negligible.
25158570	1	15	theme	tensile	272:278	arg1	testing					280:286	tensile testing	272:286	tensile testing	272:286	The influence of different additives on the mechanical properties of hydroxypropyl methylcellulose (HPMC) free films was studied using tensile testing.
25158570	0	16	theme	hydroxypropyl	100:112	arg1	films					130:134	hydroxypropyl methylcellulose films	100:134	hydroxypropyl methylcellulose films	100:134	The effect of cores and coating dispersion composition on the mechanical and adhesion properties of hydroxypropyl methylcellulose films.
25158570	6	17	theme	reduced	1086:1092	arg1	adhesion					1094:1101	reduced adhesion	1086:1101	reduced adhesion of the film coating with PEG 400	1086:1134	The addition of Mg stearate in the core led to reduced adhesion of the film coating with PEG 400, whereas the influence on the adhesion force of the film coating containing polysorbate 80 was negligible.
25158570	6	18	theme	coating	1193:1199	arg1	negligible					1231:1240	negligible	1231:1240	negligible	1231:1240	The addition of Mg stearate in the core led to reduced adhesion of the film coating with PEG 400, whereas the influence on the adhesion force of the film coating containing polysorbate 80 was negligible.
25158570	6	18	theme	coating	1193:1199	arg1	influence					1149:1157	the influence	1145:1157	the influence on the adhesion force of the film coating containing polysorbate 80	1145:1225	The addition of Mg stearate in the core led to reduced adhesion of the film coating with PEG 400, whereas the influence on the adhesion force of the film coating containing polysorbate 80 was negligible.
25158570	6	19	from	addition	1043:1050	arg1	core					1074:1077	the core	1070:1077	the core	1070:1077	The addition of Mg stearate in the core led to reduced adhesion of the film coating with PEG 400, whereas the influence on the adhesion force of the film coating containing polysorbate 80 was negligible.
25158570	2	20	theme	tensile	369:375	arg1	strength					377:384	their tensile strength	363:384	their tensile strength	363:384	Free films were prepared using the cast method and sliced into bands, and their tensile strength and maximal elongation at break was measured.
25158570	4	21	theme	coating	811:817	arg1	formulations					819:830	coating formulations	811:830	coating formulations containing PEG 400 and polysorbate 80	811:868	Tablet cores composed of microcrystalline cellulose and lactose with and without Mg stearate and compressed at three different compression forces were tested for wettability with coating formulations containing PEG 400 and polysorbate 80.
25158570	5	22	with	formulations	875:886	arg1	stearate					899:906	no Mg stearate	893:906	no Mg stearate added	893:912	For formulations with no Mg stearate added, the contact angle decreased with increasing core hardness and it also coincided with greater adhesion force of the coating.
25158570	3	23	theme	coating	508:514	arg1	formulation					516:526	the coating formulation	504:526	the coating formulation	504:526	The results showed that the addition of PEG 400 and polysorbate 80 into the coating formulation had the most influence on the films' mechanical properties compared to the HPMC film used as a control.
25158570	6	24	theme	Mg	1055:1056	arg1	stearate					1058:1065	Mg stearate	1055:1065	Mg stearate	1055:1065	The addition of Mg stearate in the core led to reduced adhesion of the film coating with PEG 400, whereas the influence on the adhesion force of the film coating containing polysorbate 80 was negligible.
25158570	6	25	theme	film	1110:1113	arg1	adhesion					1094:1101	reduced adhesion	1086:1101	reduced adhesion of the film coating with PEG 400	1086:1134	The addition of Mg stearate in the core led to reduced adhesion of the film coating with PEG 400, whereas the influence on the adhesion force of the film coating containing polysorbate 80 was negligible.
25158570	1	26	theme	mechanical	181:190	arg1	properties					192:201	the mechanical properties	177:201	the mechanical properties of hydroxypropyl methylcellulose (HPMC) free films	177:252	The influence of different additives on the mechanical properties of hydroxypropyl methylcellulose (HPMC) free films was studied using tensile testing.
25158570	0	27	theme	cores	14:18	arg1	effect					4:9	The effect	0:9	The effect of cores and coating dispersion composition on the mechanical and adhesion properties of hydroxypropyl methylcellulose films.	0:135	The effect of cores and coating dispersion composition on the mechanical and adhesion properties of hydroxypropyl methylcellulose films.
25158570	0	28	theme	films	130:134	arg1	properties					86:95	the mechanical and adhesion properties	58:95	properties	86:95	The effect of cores and coating dispersion composition on the mechanical and adhesion properties of hydroxypropyl methylcellulose films.
25158570	5	29	theme	contact	919:925	arg1	angle					927:931	the contact angle	915:931	the contact angle	915:931	For formulations with no Mg stearate added, the contact angle decreased with increasing core hardness and it also coincided with greater adhesion force of the coating.
25158570	0	30	theme	methylcellulose	114:128	arg1	films					130:134	hydroxypropyl methylcellulose films	100:134	hydroxypropyl methylcellulose films	100:134	The effect of cores and coating dispersion composition on the mechanical and adhesion properties of hydroxypropyl methylcellulose films.
25158570	3	31	theme	polysorbate	484:494	arg1	addition					460:467	the addition	456:467	the addition of PEG 400 and polysorbate 80 into the coating formulation	456:526	The results showed that the addition of PEG 400 and polysorbate 80 into the coating formulation had the most influence on the films' mechanical properties compared to the HPMC film used as a control.
25158570	0	32	theme	coating	24:30	arg1	composition					43:53	coating dispersion composition	24:53	coating dispersion composition	24:53	The effect of cores and coating dispersion composition on the mechanical and adhesion properties of hydroxypropyl methylcellulose films.
25158570	4	33	theme	Tablet	632:637	arg1	cores					639:643	Tablet cores	632:643	Tablet cores composed of microcrystalline cellulose and lactose with and without Mg stearate and compressed at three different compression forces	632:776	Tablet cores composed of microcrystalline cellulose and lactose with and without Mg stearate and compressed at three different compression forces were tested for wettability with coating formulations containing PEG 400 and polysorbate 80.
25158570	7	34	theme	tablet	1308:1313	arg1	formulation					1320:1330	the tablet core formulation	1304:1330	the tablet core formulation	1304:1330	The results also show that the adhesion force, regardless of the tablet core formulation, is highest at medium core hardness.
25158570	6	35	theme	film	1188:1191	arg1	coating					1193:1199	the film coating	1184:1199	the film coating containing polysorbate 80	1184:1225	The addition of Mg stearate in the core led to reduced adhesion of the film coating with PEG 400, whereas the influence on the adhesion force of the film coating containing polysorbate 80 was negligible.
25158570	3	36	theme	HPMC	603:606	arg1	film					608:611	the HPMC film	599:611	the HPMC film used as a control	599:629	The results showed that the addition of PEG 400 and polysorbate 80 into the coating formulation had the most influence on the films' mechanical properties compared to the HPMC film used as a control.
25158570	3	37	theme	mechanical	565:574	arg1	properties					576:585	the films' mechanical properties	554:585	the films' mechanical properties	554:585	The results showed that the addition of PEG 400 and polysorbate 80 into the coating formulation had the most influence on the films' mechanical properties compared to the HPMC film used as a control.
25158570	2	38	theme	maximal	390:396	arg1	elongation					398:407	maximal elongation	390:407	maximal elongation	390:407	Free films were prepared using the cast method and sliced into bands, and their tensile strength and maximal elongation at break was measured.
25158570	0	39	theme	composition	43:53	arg1	effect					4:9	The effect	0:9	The effect of cores and coating dispersion composition on the mechanical and adhesion properties of hydroxypropyl methylcellulose films.	0:135	The effect of cores and coating dispersion composition on the mechanical and adhesion properties of hydroxypropyl methylcellulose films.
25158570	7	40	theme	core	1315:1318	arg1	formulation					1320:1330	the tablet core formulation	1304:1330	the tablet core formulation	1304:1330	The results also show that the adhesion force, regardless of the tablet core formulation, is highest at medium core hardness.
25158570	5	41	theme	greater	1000:1006	arg1	force					1017:1021	greater adhesion force	1000:1021	greater adhesion force of the coating	1000:1036	For formulations with no Mg stearate added, the contact angle decreased with increasing core hardness and it also coincided with greater adhesion force of the coating.
25158570	3	42	theme	PEG	472:474	arg1	addition					460:467	the addition	456:467	the addition of PEG 400 and polysorbate 80 into the coating formulation	456:526	The results showed that the addition of PEG 400 and polysorbate 80 into the coating formulation had the most influence on the films' mechanical properties compared to the HPMC film used as a control.
25158570	0	43	theme	dispersion	32:41	arg1	composition					43:53	coating dispersion composition	24:53	coating dispersion composition	24:53	The effect of cores and coating dispersion composition on the mechanical and adhesion properties of hydroxypropyl methylcellulose films.
25158570	3	44	contain	had	528:530	arg1	addition					460:467	the addition	456:467	the addition of PEG 400 and polysorbate 80 into the coating formulation	456:526	The results showed that the addition of PEG 400 and polysorbate 80 into the coating formulation had the most influence on the films' mechanical properties compared to the HPMC film used as a control.
25158570	3	44	contain	had	528:530	arg2	influence					541:549	the most influence	532:549	the most influence on the films' mechanical properties	532:585	The results showed that the addition of PEG 400 and polysorbate 80 into the coating formulation had the most influence on the films' mechanical properties compared to the HPMC film used as a control.
25158570	5	45	theme	adhesion	1008:1015	arg1	force					1017:1021	greater adhesion force	1000:1021	greater adhesion force of the coating	1000:1036	For formulations with no Mg stearate added, the contact angle decreased with increasing core hardness and it also coincided with greater adhesion force of the coating.
25158570	5	46	theme	Mg	896:897	arg1	stearate					899:906	no Mg stearate	893:906	no Mg stearate added	893:912	For formulations with no Mg stearate added, the contact angle decreased with increasing core hardness and it also coincided with greater adhesion force of the coating.
25158570	4	47	contain	containing	832:841	arg2	polysorbate					855:865	polysorbate 80	855:868	polysorbate 80	855:868	Tablet cores composed of microcrystalline cellulose and lactose with and without Mg stearate and compressed at three different compression forces were tested for wettability with coating formulations containing PEG 400 and polysorbate 80.
25158570	4	47	contain	containing	832:841	arg2	PEG					843:845	PEG 400	843:849	PEG 400	843:849	Tablet cores composed of microcrystalline cellulose and lactose with and without Mg stearate and compressed at three different compression forces were tested for wettability with coating formulations containing PEG 400 and polysorbate 80.
25158570	4	47	contain	containing	832:841	arg1	formulations					819:830	coating formulations	811:830	coating formulations containing PEG 400 and polysorbate 80	811:868	Tablet cores composed of microcrystalline cellulose and lactose with and without Mg stearate and compressed at three different compression forces were tested for wettability with coating formulations containing PEG 400 and polysorbate 80.
25158570	4	48	theme	compression	759:769	arg1	forces					771:776	three different compression forces	743:776	three different compression forces	743:776	Tablet cores composed of microcrystalline cellulose and lactose with and without Mg stearate and compressed at three different compression forces were tested for wettability with coating formulations containing PEG 400 and polysorbate 80.
25158570	6	49	theme	adhesion	1166:1173	arg1	force					1175:1179	the adhesion force	1162:1179	the adhesion force	1162:1179	The addition of Mg stearate in the core led to reduced adhesion of the film coating with PEG 400, whereas the influence on the adhesion force of the film coating containing polysorbate 80 was negligible.
25158570	4	50	dep	cores	639:643	arg1	compressed					729:738	compressed	729:738	compressed at three different compression forces	729:776	Tablet cores composed of microcrystalline cellulose and lactose with and without Mg stearate and compressed at three different compression forces were tested for wettability with coating formulations containing PEG 400 and polysorbate 80.
25158570	4	50	dep	cores	639:643	arg1	composed					645:652	composed	645:652	composed of microcrystalline cellulose and lactose with and without Mg stearate	645:723	Tablet cores composed of microcrystalline cellulose and lactose with and without Mg stearate and compressed at three different compression forces were tested for wettability with coating formulations containing PEG 400 and polysorbate 80.
25158570	4	51	theme	different	749:757	arg1	forces					771:776	three different compression forces	743:776	three different compression forces	743:776	Tablet cores composed of microcrystalline cellulose and lactose with and without Mg stearate and compressed at three different compression forces were tested for wettability with coating formulations containing PEG 400 and polysorbate 80.
25158570	4	52	theme	Mg	713:714	arg1	stearate					716:723	Mg stearate	713:723	Mg stearate	713:723	Tablet cores composed of microcrystalline cellulose and lactose with and without Mg stearate and compressed at three different compression forces were tested for wettability with coating formulations containing PEG 400 and polysorbate 80.
25158570	2	53	theme	cast	324:327	arg1	method					329:334	the cast method	320:334	the cast method	320:334	Free films were prepared using the cast method and sliced into bands, and their tensile strength and maximal elongation at break was measured.
25158570	3	54	from	influence	541:549	arg1	properties					576:585	the films' mechanical properties	554:585	the films' mechanical properties	554:585	The results showed that the addition of PEG 400 and polysorbate 80 into the coating formulation had the most influence on the films' mechanical properties compared to the HPMC film used as a control.
25158570	4	55	with	wettability	794:804	arg1	formulations					819:830	coating formulations	811:830	coating formulations containing PEG 400 and polysorbate 80	811:868	Tablet cores composed of microcrystalline cellulose and lactose with and without Mg stearate and compressed at three different compression forces were tested for wettability with coating formulations containing PEG 400 and polysorbate 80.
25158570	5	56	theme	coating	1030:1036	arg1	force					1017:1021	greater adhesion force	1000:1021	greater adhesion force of the coating	1000:1036	For formulations with no Mg stearate added, the contact angle decreased with increasing core hardness and it also coincided with greater adhesion force of the coating.
25158570	7	57	theme	adhesion	1274:1281	arg1	highest					1336:1342	highest	1336:1342	highest	1336:1342	The results also show that the adhesion force, regardless of the tablet core formulation, is highest at medium core hardness.
25158570	7	57	theme	adhesion	1274:1281	arg1	force					1283:1287	the adhesion force	1270:1287	the adhesion force	1270:1287	The results also show that the adhesion force, regardless of the tablet core formulation, is highest at medium core hardness.
25158570	1	58	theme	free	243:246	arg1	films					248:252	hydroxypropyl methylcellulose (HPMC) free films	206:252	hydroxypropyl methylcellulose (HPMC) free films	206:252	The influence of different additives on the mechanical properties of hydroxypropyl methylcellulose (HPMC) free films was studied using tensile testing.
25158570	2	59	from	break	412:416	arg1	strength					377:384	their tensile strength	363:384	their tensile strength	363:384	Free films were prepared using the cast method and sliced into bands, and their tensile strength and maximal elongation at break was measured.
25158570	2	59	from	break	412:416	arg1	elongation					398:407	maximal elongation	390:407	maximal elongation	390:407	Free films were prepared using the cast method and sliced into bands, and their tensile strength and maximal elongation at break was measured.
25158570	1	60	theme	films	248:252	arg1	properties					192:201	the mechanical properties	177:201	the mechanical properties of hydroxypropyl methylcellulose (HPMC) free films	177:252	The influence of different additives on the mechanical properties of hydroxypropyl methylcellulose (HPMC) free films was studied using tensile testing.
28375595	6	0	theme	practical	1207:1215	arg1	application					1217:1227	practical application	1207:1227	practical application of oligochitosan in pharmaceutical, cosmetic, and food compositions	1207:1295	These results are promising for expanding practical application of oligochitosan in pharmaceutical, cosmetic, and food compositions.
28375595	1	1	theme	acidic	156:161	arg1	media					171:175	acidic aqueous media	156:175	acidic aqueous media	156:175	Oligochitosan (short chain chitosan) is more soluble in acidic aqueous media than a high molecular weight (MW) chitosan, but its antimicrobial activity decreases with increase in degree of acetylation (DA) and increase in pH above a critical pH threshold point.
28375595	0	2	theme	Activity	90:97	arg1	Dependence					33:42	pH Dependence	30:42	N-Reacetylated Oligochitosan: pH Dependence of Self-Assembly Properties and Antibacterial Activity.	0:98	N-Reacetylated Oligochitosan: pH Dependence of Self-Assembly Properties and Antibacterial Activity.
28375595	2	3	theme	pH	584:585	arg1	range					587:591	a wide pH range	577:591	a wide pH range as a function of MW and DA	577:618	In the present study, oligochitosans varying in MW were additionally N-acetylated and their self-assembly properties and antibacterial activity toward Staphylococcus aureus and Escherichia coli were investigated in a wide pH range as a function of MW and DA.
28375595	2	4	theme	present	369:375	arg1	study					377:381	the present study	365:381	the present study	365:381	In the present study, oligochitosans varying in MW were additionally N-acetylated and their self-assembly properties and antibacterial activity toward Staphylococcus aureus and Escherichia coli were investigated in a wide pH range as a function of MW and DA.
28375595	1	5	theme	aqueous	163:169	arg1	media					171:175	acidic aqueous media	156:175	acidic aqueous media	156:175	Oligochitosan (short chain chitosan) is more soluble in acidic aqueous media than a high molecular weight (MW) chitosan, but its antimicrobial activity decreases with increase in degree of acetylation (DA) and increase in pH above a critical pH threshold point.
28375595	3	6	with	oligochitosan	672:684	arg1	Mw					691:692	Mw	691:692	Mw	691:692	Light scattering studies reveals that reacetyleted oligochitosan with Mw ≤ 11 kDa is completely soluble in alkaline media (up to pH 12.5), if its DA is not less than 16%.
28375595	1	7	theme	antimicrobial	229:241	arg1	activity					243:250	its antimicrobial activity	225:250	its antimicrobial activity	225:250	Oligochitosan (short chain chitosan) is more soluble in acidic aqueous media than a high molecular weight (MW) chitosan, but its antimicrobial activity decreases with increase in degree of acetylation (DA) and increase in pH above a critical pH threshold point.
28375595	3	8	theme	reacetyleted	659:670	arg1	oligochitosan					672:684	reacetyleted oligochitosan	659:684	reacetyleted oligochitosan with Mw ≤ 11 kDa	659:701	Light scattering studies reveals that reacetyleted oligochitosan with Mw ≤ 11 kDa is completely soluble in alkaline media (up to pH 12.5), if its DA is not less than 16%.
28375595	2	9	theme	wide	579:582	arg1	range					587:591	a wide pH range	577:591	a wide pH range as a function of MW and DA	577:618	In the present study, oligochitosans varying in MW were additionally N-acetylated and their self-assembly properties and antibacterial activity toward Staphylococcus aureus and Escherichia coli were investigated in a wide pH range as a function of MW and DA.
28375595	2	10	theme	DA	617:618	arg1	function					598:605	a function	596:605	a function of MW and DA	596:618	In the present study, oligochitosans varying in MW were additionally N-acetylated and their self-assembly properties and antibacterial activity toward Staphylococcus aureus and Escherichia coli were investigated in a wide pH range as a function of MW and DA.
28375595	5	11	theme	reacetylated	1080:1091	arg1	oligochitosans					1093:1106	reacetylated oligochitosans	1080:1106	reacetylated oligochitosans	1080:1106	Considering the influence of DA and MW, the antibacterial activity of reacetylated oligochitosans is maximal in the short interval of DA 16-28% at pH 7.4.
28375595	1	12	from	soluble	145:151	arg1	media					171:175	acidic aqueous media	156:175	acidic aqueous media	156:175	Oligochitosan (short chain chitosan) is more soluble in acidic aqueous media than a high molecular weight (MW) chitosan, but its antimicrobial activity decreases with increase in degree of acetylation (DA) and increase in pH above a critical pH threshold point.
28375595	5	13	theme	DA	1144:1145	arg1	%					1152:1152	DA 16-28%	1144:1152	DA 16-28%	1144:1152	Considering the influence of DA and MW, the antibacterial activity of reacetylated oligochitosans is maximal in the short interval of DA 16-28% at pH 7.4.
28375595	5	14	theme	DA	1039:1040	arg1	influence					1026:1034	the influence	1022:1034	the influence of DA and MW	1022:1047	Considering the influence of DA and MW, the antibacterial activity of reacetylated oligochitosans is maximal in the short interval of DA 16-28% at pH 7.4.
28375595	2	15	theme	antibacterial	483:495	arg1	activity					497:504	antibacterial activity	483:504	antibacterial activity toward Staphylococcus aureus and Escherichia coli	483:554	In the present study, oligochitosans varying in MW were additionally N-acetylated and their self-assembly properties and antibacterial activity toward Staphylococcus aureus and Escherichia coli were investigated in a wide pH range as a function of MW and DA.
28375595	3	16	dep	media	737:741	arg1	up					744:745	up	744:745	up to pH 12.5	744:756	Light scattering studies reveals that reacetyleted oligochitosan with Mw ≤ 11 kDa is completely soluble in alkaline media (up to pH 12.5), if its DA is not less than 16%.
28375595	2	17	theme	MW	610:611	arg1	function					598:605	a function	596:605	a function of MW and DA	596:618	In the present study, oligochitosans varying in MW were additionally N-acetylated and their self-assembly properties and antibacterial activity toward Staphylococcus aureus and Escherichia coli were investigated in a wide pH range as a function of MW and DA.
28375595	4	18	theme	pH	972:973	arg1	≥					975:975	pH ≥ 8	972:977	pH ≥ 8	972:977	Reacetylated chitosans with DA ∼ 30% are solubile in the entire pH range up to 12.5, if their Mw is not higher than 25 kDa, but they aggregate and precipitate from the solution at pH ≥ 8 when their Mw is above 25 kDa.
28375595	4	19	theme	entire	849:854	arg1	range					859:863	the entire pH range	845:863	the entire pH range up to 12.5	845:874	Reacetylated chitosans with DA ∼ 30% are solubile in the entire pH range up to 12.5, if their Mw is not higher than 25 kDa, but they aggregate and precipitate from the solution at pH ≥ 8 when their Mw is above 25 kDa.
28375595	2	20	from	N-acetylated	431:442	arg1	study					377:381	the present study	365:381	the present study	365:381	In the present study, oligochitosans varying in MW were additionally N-acetylated and their self-assembly properties and antibacterial activity toward Staphylococcus aureus and Escherichia coli were investigated in a wide pH range as a function of MW and DA.
28375595	5	21	theme	%	1152:1152	arg1	interval					1132:1139	the short interval	1122:1139	the short interval of DA 16-28% at pH 7.4	1122:1162	Considering the influence of DA and MW, the antibacterial activity of reacetylated oligochitosans is maximal in the short interval of DA 16-28% at pH 7.4.
28375595	4	22	theme	pH	856:857	arg1	range					859:863	the entire pH range	845:863	the entire pH range up to 12.5	845:874	Reacetylated chitosans with DA ∼ 30% are solubile in the entire pH range up to 12.5, if their Mw is not higher than 25 kDa, but they aggregate and precipitate from the solution at pH ≥ 8 when their Mw is above 25 kDa.
28375595	1	23	theme	critical	333:340	arg1	point					355:359	a critical pH threshold point	331:359	a critical pH threshold point	331:359	Oligochitosan (short chain chitosan) is more soluble in acidic aqueous media than a high molecular weight (MW) chitosan, but its antimicrobial activity decreases with increase in degree of acetylation (DA) and increase in pH above a critical pH threshold point.
28375595	3	24	from	soluble	717:723	arg1	media					737:741	alkaline media	728:741	alkaline media (up to pH 12.5)	728:757	Light scattering studies reveals that reacetyleted oligochitosan with Mw ≤ 11 kDa is completely soluble in alkaline media (up to pH 12.5), if its DA is not less than 16%.
28375595	5	25	from	maximal	1111:1117	arg1	interval					1132:1139	the short interval	1122:1139	the short interval of DA 16-28% at pH 7.4	1122:1162	Considering the influence of DA and MW, the antibacterial activity of reacetylated oligochitosans is maximal in the short interval of DA 16-28% at pH 7.4.
28375595	4	26	from	≥	975:975	arg1	solution					960:967	the solution	956:967	the solution at pH ≥ 8	956:977	Reacetylated chitosans with DA ∼ 30% are solubile in the entire pH range up to 12.5, if their Mw is not higher than 25 kDa, but they aggregate and precipitate from the solution at pH ≥ 8 when their Mw is above 25 kDa.
28375595	0	27	theme	N-Reacetylated	0:13	arg1	Oligochitosan					15:27	N-Reacetylated Oligochitosan	0:27	N-Reacetylated Oligochitosan: pH Dependence of Self-Assembly Properties and Antibacterial Activity.	0:98	N-Reacetylated Oligochitosan: pH Dependence of Self-Assembly Properties and Antibacterial Activity.
28375595	1	28	from	increase	267:274	arg1	degree					279:284	degree	279:284	degree of acetylation (DA)	279:304	Oligochitosan (short chain chitosan) is more soluble in acidic aqueous media than a high molecular weight (MW) chitosan, but its antimicrobial activity decreases with increase in degree of acetylation (DA) and increase in pH above a critical pH threshold point.
28375595	1	28	from	increase	267:274	arg1	pH					322:323	pH	322:323	pH above a critical pH threshold point	322:359	Oligochitosan (short chain chitosan) is more soluble in acidic aqueous media than a high molecular weight (MW) chitosan, but its antimicrobial activity decreases with increase in degree of acetylation (DA) and increase in pH above a critical pH threshold point.
28375595	1	29	theme	high	184:187	arg1	weight					199:204	a high molecular weight	182:204	a high molecular weight (MW) chitosan	182:218	Oligochitosan (short chain chitosan) is more soluble in acidic aqueous media than a high molecular weight (MW) chitosan, but its antimicrobial activity decreases with increase in degree of acetylation (DA) and increase in pH above a critical pH threshold point.
28375595	1	29	theme	high	184:187	arg1	MW					207:208	MW	207:208	MW	207:208	Oligochitosan (short chain chitosan) is more soluble in acidic aqueous media than a high molecular weight (MW) chitosan, but its antimicrobial activity decreases with increase in degree of acetylation (DA) and increase in pH above a critical pH threshold point.
28375595	0	30	theme	pH	30:31	arg1	Dependence					33:42	pH Dependence	30:42	N-Reacetylated Oligochitosan: pH Dependence of Self-Assembly Properties and Antibacterial Activity.	0:98	N-Reacetylated Oligochitosan: pH Dependence of Self-Assembly Properties and Antibacterial Activity.
28375595	1	31	theme	short	115:119	arg1	chitosan					127:134	short chain chitosan	115:134	short chain chitosan	115:134	Oligochitosan (short chain chitosan) is more soluble in acidic aqueous media than a high molecular weight (MW) chitosan, but its antimicrobial activity decreases with increase in degree of acetylation (DA) and increase in pH above a critical pH threshold point.
28375595	1	31	theme	short	115:119	arg1	Oligochitosan					100:112	Oligochitosan	100:112	Oligochitosan (short chain chitosan)	100:135	Oligochitosan (short chain chitosan) is more soluble in acidic aqueous media than a high molecular weight (MW) chitosan, but its antimicrobial activity decreases with increase in degree of acetylation (DA) and increase in pH above a critical pH threshold point.
28375595	5	32	theme	short	1126:1130	arg1	interval					1132:1139	the short interval	1122:1139	the short interval of DA 16-28% at pH 7.4	1122:1162	Considering the influence of DA and MW, the antibacterial activity of reacetylated oligochitosans is maximal in the short interval of DA 16-28% at pH 7.4.
28375595	1	33	theme	molecular	189:197	arg1	weight					199:204	a high molecular weight	182:204	a high molecular weight (MW) chitosan	182:218	Oligochitosan (short chain chitosan) is more soluble in acidic aqueous media than a high molecular weight (MW) chitosan, but its antimicrobial activity decreases with increase in degree of acetylation (DA) and increase in pH above a critical pH threshold point.
28375595	1	33	theme	molecular	189:197	arg1	MW					207:208	MW	207:208	MW	207:208	Oligochitosan (short chain chitosan) is more soluble in acidic aqueous media than a high molecular weight (MW) chitosan, but its antimicrobial activity decreases with increase in degree of acetylation (DA) and increase in pH above a critical pH threshold point.
28375595	6	34	theme	oligochitosan	1232:1244	arg1	application					1217:1227	practical application	1207:1227	practical application of oligochitosan in pharmaceutical, cosmetic, and food compositions	1207:1295	These results are promising for expanding practical application of oligochitosan in pharmaceutical, cosmetic, and food compositions.
28375595	4	35	from	solubile	833:840	arg1	range					859:863	the entire pH range	845:863	the entire pH range up to 12.5	845:874	Reacetylated chitosans with DA ∼ 30% are solubile in the entire pH range up to 12.5, if their Mw is not higher than 25 kDa, but they aggregate and precipitate from the solution at pH ≥ 8 when their Mw is above 25 kDa.
28375595	1	36	theme	pH	342:343	arg1	point					355:359	a critical pH threshold point	331:359	a critical pH threshold point	331:359	Oligochitosan (short chain chitosan) is more soluble in acidic aqueous media than a high molecular weight (MW) chitosan, but its antimicrobial activity decreases with increase in degree of acetylation (DA) and increase in pH above a critical pH threshold point.
28375595	1	37	theme	chain	121:125	arg1	chitosan					127:134	short chain chitosan	115:134	short chain chitosan	115:134	Oligochitosan (short chain chitosan) is more soluble in acidic aqueous media than a high molecular weight (MW) chitosan, but its antimicrobial activity decreases with increase in degree of acetylation (DA) and increase in pH above a critical pH threshold point.
28375595	1	37	theme	chain	121:125	arg1	Oligochitosan					100:112	Oligochitosan	100:112	Oligochitosan (short chain chitosan)	100:135	Oligochitosan (short chain chitosan) is more soluble in acidic aqueous media than a high molecular weight (MW) chitosan, but its antimicrobial activity decreases with increase in degree of acetylation (DA) and increase in pH above a critical pH threshold point.
28375595	1	38	theme	weight	199:204	arg1	chitosan					211:218	a high molecular weight (MW) chitosan	182:218	a high molecular weight (MW) chitosan	182:218	Oligochitosan (short chain chitosan) is more soluble in acidic aqueous media than a high molecular weight (MW) chitosan, but its antimicrobial activity decreases with increase in degree of acetylation (DA) and increase in pH above a critical pH threshold point.
28375595	1	39	from	increase	310:317	arg1	degree					279:284	degree	279:284	degree of acetylation (DA)	279:304	Oligochitosan (short chain chitosan) is more soluble in acidic aqueous media than a high molecular weight (MW) chitosan, but its antimicrobial activity decreases with increase in degree of acetylation (DA) and increase in pH above a critical pH threshold point.
28375595	1	39	from	increase	310:317	arg1	pH					322:323	pH	322:323	pH above a critical pH threshold point	322:359	Oligochitosan (short chain chitosan) is more soluble in acidic aqueous media than a high molecular weight (MW) chitosan, but its antimicrobial activity decreases with increase in degree of acetylation (DA) and increase in pH above a critical pH threshold point.
28375595	1	40	theme	threshold	345:353	arg1	point					355:359	a critical pH threshold point	331:359	a critical pH threshold point	331:359	Oligochitosan (short chain chitosan) is more soluble in acidic aqueous media than a high molecular weight (MW) chitosan, but its antimicrobial activity decreases with increase in degree of acetylation (DA) and increase in pH above a critical pH threshold point.
28375595	5	41	from	interval	1132:1139	arg1	maximal					1111:1117	maximal	1111:1117	maximal	1111:1117	Considering the influence of DA and MW, the antibacterial activity of reacetylated oligochitosans is maximal in the short interval of DA 16-28% at pH 7.4.
28375595	5	41	from	interval	1132:1139	arg1	activity					1068:1075	the antibacterial activity	1050:1075	the antibacterial activity of reacetylated oligochitosans	1050:1106	Considering the influence of DA and MW, the antibacterial activity of reacetylated oligochitosans is maximal in the short interval of DA 16-28% at pH 7.4.
28375595	4	42	with	chitosans	805:813	arg1	%					827:827	DA ∼ 30%	820:827	DA ∼ 30%	820:827	Reacetylated chitosans with DA ∼ 30% are solubile in the entire pH range up to 12.5, if their Mw is not higher than 25 kDa, but they aggregate and precipitate from the solution at pH ≥ 8 when their Mw is above 25 kDa.
28375595	5	43	from	pH	1157:1158	arg1	interval					1132:1139	the short interval	1122:1139	the short interval of DA 16-28% at pH 7.4	1122:1162	Considering the influence of DA and MW, the antibacterial activity of reacetylated oligochitosans is maximal in the short interval of DA 16-28% at pH 7.4.
28375595	0	44	theme	Properties	61:70	arg1	Dependence					33:42	pH Dependence	30:42	N-Reacetylated Oligochitosan: pH Dependence of Self-Assembly Properties and Antibacterial Activity.	0:98	N-Reacetylated Oligochitosan: pH Dependence of Self-Assembly Properties and Antibacterial Activity.
28375595	1	45	theme	acetylation	289:299	arg1	degree					279:284	degree	279:284	degree of acetylation (DA)	279:304	Oligochitosan (short chain chitosan) is more soluble in acidic aqueous media than a high molecular weight (MW) chitosan, but its antimicrobial activity decreases with increase in degree of acetylation (DA) and increase in pH above a critical pH threshold point.
28375595	4	46	dep	12.5	871:874	arg1	to					868:869	to	868:869	to	868:869	Reacetylated chitosans with DA ∼ 30% are solubile in the entire pH range up to 12.5, if their Mw is not higher than 25 kDa, but they aggregate and precipitate from the solution at pH ≥ 8 when their Mw is above 25 kDa.
28375595	0	47	theme	Self-Assembly	47:59	arg1	Properties					61:70	Self-Assembly Properties	47:70	Self-Assembly Properties	47:70	N-Reacetylated Oligochitosan: pH Dependence of Self-Assembly Properties and Antibacterial Activity.
28375595	3	48	theme	alkaline	728:735	arg1	media					737:741	alkaline media	728:741	alkaline media (up to pH 12.5)	728:757	Light scattering studies reveals that reacetyleted oligochitosan with Mw ≤ 11 kDa is completely soluble in alkaline media (up to pH 12.5), if its DA is not less than 16%.
28375595	6	49	dep	pharmaceutical	1249:1262	arg1	compositions					1284:1295	compositions	1284:1295	compositions	1284:1295	These results are promising for expanding practical application of oligochitosan in pharmaceutical, cosmetic, and food compositions.
28375595	4	50	theme	Reacetylated	792:803	arg1	chitosans					805:813	Reacetylated chitosans	792:813	Reacetylated chitosans with DA ∼ 30%	792:827	Reacetylated chitosans with DA ∼ 30% are solubile in the entire pH range up to 12.5, if their Mw is not higher than 25 kDa, but they aggregate and precipitate from the solution at pH ≥ 8 when their Mw is above 25 kDa.
28375595	5	51	theme	oligochitosans	1093:1106	arg1	maximal					1111:1117	maximal	1111:1117	maximal	1111:1117	Considering the influence of DA and MW, the antibacterial activity of reacetylated oligochitosans is maximal in the short interval of DA 16-28% at pH 7.4.
28375595	5	51	theme	oligochitosans	1093:1106	arg1	activity					1068:1075	the antibacterial activity	1050:1075	the antibacterial activity of reacetylated oligochitosans	1050:1106	Considering the influence of DA and MW, the antibacterial activity of reacetylated oligochitosans is maximal in the short interval of DA 16-28% at pH 7.4.
28375595	6	52	from	application	1217:1227	arg1	cosmetic					1265:1272	cosmetic	1265:1272	cosmetic	1265:1272	These results are promising for expanding practical application of oligochitosan in pharmaceutical, cosmetic, and food compositions.
28375595	6	52	from	application	1217:1227	arg1	food					1279:1282	food	1279:1282	food	1279:1282	These results are promising for expanding practical application of oligochitosan in pharmaceutical, cosmetic, and food compositions.
28375595	6	52	from	application	1217:1227	arg1	pharmaceutical					1249:1262	pharmaceutical	1249:1262	pharmaceutical	1249:1262	These results are promising for expanding practical application of oligochitosan in pharmaceutical, cosmetic, and food compositions.
28375595	4	53	theme	∼	823:823	arg1	%					827:827	DA ∼ 30%	820:827	DA ∼ 30%	820:827	Reacetylated chitosans with DA ∼ 30% are solubile in the entire pH range up to 12.5, if their Mw is not higher than 25 kDa, but they aggregate and precipitate from the solution at pH ≥ 8 when their Mw is above 25 kDa.
28375595	2	54	from	study	377:381	arg1	N-acetylated					431:442	N-acetylated	431:442	N-acetylated	431:442	In the present study, oligochitosans varying in MW were additionally N-acetylated and their self-assembly properties and antibacterial activity toward Staphylococcus aureus and Escherichia coli were investigated in a wide pH range as a function of MW and DA.
28375595	4	55	theme	DA	820:821	arg1	%					827:827	DA ∼ 30%	820:827	DA ∼ 30%	820:827	Reacetylated chitosans with DA ∼ 30% are solubile in the entire pH range up to 12.5, if their Mw is not higher than 25 kDa, but they aggregate and precipitate from the solution at pH ≥ 8 when their Mw is above 25 kDa.
28375595	3	56	theme	Light	621:625	arg1	studies					638:644	Light scattering studies	621:644	Light scattering studies	621:644	Light scattering studies reveals that reacetyleted oligochitosan with Mw ≤ 11 kDa is completely soluble in alkaline media (up to pH 12.5), if its DA is not less than 16%.
28375595	3	57	from	media	737:741	arg1	soluble					717:723	soluble	717:723	soluble	717:723	Light scattering studies reveals that reacetyleted oligochitosan with Mw ≤ 11 kDa is completely soluble in alkaline media (up to pH 12.5), if its DA is not less than 16%.
28375595	5	58	theme	MW	1046:1047	arg1	influence					1026:1034	the influence	1022:1034	the influence of DA and MW	1022:1047	Considering the influence of DA and MW, the antibacterial activity of reacetylated oligochitosans is maximal in the short interval of DA 16-28% at pH 7.4.
28375595	0	59	theme	Antibacterial	76:88	arg1	Activity					90:97	Antibacterial Activity	76:97	Antibacterial Activity	76:97	N-Reacetylated Oligochitosan: pH Dependence of Self-Assembly Properties and Antibacterial Activity.
28375595	3	60	theme	scattering	627:636	arg1	studies					638:644	Light scattering studies	621:644	Light scattering studies	621:644	Light scattering studies reveals that reacetyleted oligochitosan with Mw ≤ 11 kDa is completely soluble in alkaline media (up to pH 12.5), if its DA is not less than 16%.
28375595	0	61	dep	Oligochitosan	15:27	arg1	Dependence					33:42	pH Dependence	30:42	N-Reacetylated Oligochitosan: pH Dependence of Self-Assembly Properties and Antibacterial Activity.	0:98	N-Reacetylated Oligochitosan: pH Dependence of Self-Assembly Properties and Antibacterial Activity.
28375595	2	62	theme	self-assembly	454:466	arg1	properties					468:477	their self-assembly properties	448:477	their self-assembly properties	448:477	In the present study, oligochitosans varying in MW were additionally N-acetylated and their self-assembly properties and antibacterial activity toward Staphylococcus aureus and Escherichia coli were investigated in a wide pH range as a function of MW and DA.
28375595	1	63	from	media	171:175	arg1	soluble					145:151	soluble	145:151	soluble	145:151	Oligochitosan (short chain chitosan) is more soluble in acidic aqueous media than a high molecular weight (MW) chitosan, but its antimicrobial activity decreases with increase in degree of acetylation (DA) and increase in pH above a critical pH threshold point.
28375595	5	64	theme	antibacterial	1054:1066	arg1	maximal					1111:1117	maximal	1111:1117	maximal	1111:1117	Considering the influence of DA and MW, the antibacterial activity of reacetylated oligochitosans is maximal in the short interval of DA 16-28% at pH 7.4.
28375595	5	64	theme	antibacterial	1054:1066	arg1	activity					1068:1075	the antibacterial activity	1050:1075	the antibacterial activity of reacetylated oligochitosans	1050:1106	Considering the influence of DA and MW, the antibacterial activity of reacetylated oligochitosans is maximal in the short interval of DA 16-28% at pH 7.4.
28375595	4	65	from	range	859:863	arg1	solubile					833:840	solubile	833:840	solubile	833:840	Reacetylated chitosans with DA ∼ 30% are solubile in the entire pH range up to 12.5, if their Mw is not higher than 25 kDa, but they aggregate and precipitate from the solution at pH ≥ 8 when their Mw is above 25 kDa.
28732886	0	0	theme	enhanced	112:119	arg1	healing					127:133	enhanced wound healing	112:133	enhanced wound healing	112:133	Chitosan-hyaluronic acid composite sponge scaffold enriched with Andrographolide-loaded lipid nanoparticles for enhanced wound healing.
28732886	8	1	with	healing	1020:1026	arg1	scar					1036:1039	no scar	1033:1039	no scar	1033:1039	The in vivo evaluation in rats reveals that the chitosan-hyaluronic acid/NLC4 sponge enhances the wound healing with no scar and improved tissue quality.
28732886	8	1	with	healing	1020:1026	arg1	quality					1061:1067	improved tissue quality	1045:1067	improved tissue quality	1045:1067	The in vivo evaluation in rats reveals that the chitosan-hyaluronic acid/NLC4 sponge enhances the wound healing with no scar and improved tissue quality.
28732886	4	2	theme	prolonged	540:548	arg1	release					550:556	a prolonged release	538:556	a prolonged release of AND up to 48-h	538:574	Itexhibited spherical shape with 253nm, 83.04% entrapment efficiency and a prolonged release of AND up to 48-h.
28732886	5	3	theme	wound	760:764	arg1	sites					766:770	wound sites	760:770	wound sites	760:770	Consecutively, NLC4 was incorporated in a chitosan-hyaluronic acid gel and lyophilized for 24h to obtain chitosan-hyaluronic acid/NLC4 nanocomposite sponge to enhance AND delivery to wound sites.
28732886	8	4	dep	in	920:921	arg1	vivo					923:926	vivo	923:926	vivo	923:926	The in vivo evaluation in rats reveals that the chitosan-hyaluronic acid/NLC4 sponge enhances the wound healing with no scar and improved tissue quality.
28732886	0	5	theme	wound	121:125	arg1	healing					127:133	enhanced wound healing	112:133	enhanced wound healing	112:133	Chitosan-hyaluronic acid composite sponge scaffold enriched with Andrographolide-loaded lipid nanoparticles for enhanced wound healing.
28732886	4	6	theme	83.04	505:509	arg1	%					510:510	%	510:510	%	510:510	Itexhibited spherical shape with 253nm, 83.04% entrapment efficiency and a prolonged release of AND up to 48-h.
28732886	5	7	theme	chitosan-hyaluronic	682:700	arg1	sponge					726:731	chitosan-hyaluronic acid/NLC4 nanocomposite sponge	682:731	chitosan-hyaluronic acid/NLC4 nanocomposite sponge	682:731	Consecutively, NLC4 was incorporated in a chitosan-hyaluronic acid gel and lyophilized for 24h to obtain chitosan-hyaluronic acid/NLC4 nanocomposite sponge to enhance AND delivery to wound sites.
28732886	5	8	theme	chitosan-hyaluronic	619:637	arg1	gel					644:646	a chitosan-hyaluronic acid gel	617:646	a chitosan-hyaluronic acid gel	617:646	Consecutively, NLC4 was incorporated in a chitosan-hyaluronic acid gel and lyophilized for 24h to obtain chitosan-hyaluronic acid/NLC4 nanocomposite sponge to enhance AND delivery to wound sites.
28732886	7	9	theme	enhanced	897:904	arg1	swelling					906:913	enhanced swelling	897:913	enhanced swelling	897:913	Nanocomposites showed a porosity of 56.22% with enhanced swelling.
28732886	9	10	theme	chitosan-hyaluronic	1137:1155	arg1	sponge					1167:1172	this novel chitosan-hyaluronic acid/NLC4 sponge	1126:1172	this novel chitosan-hyaluronic acid/NLC4 sponge for wound care application	1126:1199	These results strongly support the possibility of using this novel chitosan-hyaluronic acid/NLC4 sponge for wound care application.
28732886	5	11	theme	acid	639:642	arg1	gel					644:646	a chitosan-hyaluronic acid gel	617:646	a chitosan-hyaluronic acid gel	617:646	Consecutively, NLC4 was incorporated in a chitosan-hyaluronic acid gel and lyophilized for 24h to obtain chitosan-hyaluronic acid/NLC4 nanocomposite sponge to enhance AND delivery to wound sites.
28732886	4	12	theme	entrapment	512:521	arg1	efficiency					523:532	83.04% entrapment efficiency	505:532	83.04% entrapment efficiency	505:532	Itexhibited spherical shape with 253nm, 83.04% entrapment efficiency and a prolonged release of AND up to 48-h.
28732886	6	13	theme	electron	828:835	arg1	microscopy					837:846	scanning electron microscopy	819:846	scanning electron microscopy	819:846	The morphology of sponge was characterized by scanning electron microscopy.
28732886	7	14	theme	%	890:890	arg1	porosity					873:880	a porosity	871:880	a porosity of 56.22% with enhanced swelling	871:913	Nanocomposites showed a porosity of 56.22% with enhanced swelling.
28732886	0	15	theme	acid	20:23	arg1	scaffold					42:49	Chitosan-hyaluronic acid composite sponge scaffold	0:49	Chitosan-hyaluronic acid composite sponge scaffold	0:49	Chitosan-hyaluronic acid composite sponge scaffold enriched with Andrographolide-loaded lipid nanoparticles for enhanced wound healing.
28732886	4	16	theme	%	510:510	arg1	efficiency					523:532	83.04% entrapment efficiency	505:532	83.04% entrapment efficiency	505:532	Itexhibited spherical shape with 253nm, 83.04% entrapment efficiency and a prolonged release of AND up to 48-h.
28732886	6	17	theme	scanning	819:826	arg1	microscopy					837:846	scanning electron microscopy	819:846	scanning electron microscopy	819:846	The morphology of sponge was characterized by scanning electron microscopy.
28732886	1	18	dep	scaffold	191:198	arg1	enriched					200:207	enriched	200:207	enriched with Andrographolide (AND) lipid nanocarriers	200:253	In this work chitosan-hyaluronic acid composite sponge scaffold enriched with Andrographolide (AND) lipid nanocarriers was developed.
28732886	8	19	theme	in	920:921	arg1	evaluation					928:937	The in vivo evaluation	916:937	The in vivo evaluation in rats	916:945	The in vivo evaluation in rats reveals that the chitosan-hyaluronic acid/NLC4 sponge enhances the wound healing with no scar and improved tissue quality.
28732886	3	20	theme	optimal	444:450	arg1	it					424:425	it	424:425	it	424:425	NLC4 had the highest desirability value (0.882) and therefore, it was chosen as an optimal nanocarrier.
28732886	3	20	theme	optimal	444:450	arg1	nanocarrier					452:462	an optimal nanocarrier	441:462	an optimal nanocarrier	441:462	NLC4 had the highest desirability value (0.882) and therefore, it was chosen as an optimal nanocarrier.
28732886	0	21	theme	Chitosan-hyaluronic	0:18	arg1	scaffold					42:49	Chitosan-hyaluronic acid composite sponge scaffold	0:49	Chitosan-hyaluronic acid composite sponge scaffold	0:49	Chitosan-hyaluronic acid composite sponge scaffold enriched with Andrographolide-loaded lipid nanoparticles for enhanced wound healing.
28732886	9	22	theme	acid/NLC4	1157:1165	arg1	sponge					1167:1172	this novel chitosan-hyaluronic acid/NLC4 sponge	1126:1172	this novel chitosan-hyaluronic acid/NLC4 sponge for wound care application	1126:1199	These results strongly support the possibility of using this novel chitosan-hyaluronic acid/NLC4 sponge for wound care application.
28732886	0	23	theme	sponge	35:40	arg1	scaffold					42:49	Chitosan-hyaluronic acid composite sponge scaffold	0:49	Chitosan-hyaluronic acid composite sponge scaffold	0:49	Chitosan-hyaluronic acid composite sponge scaffold enriched with Andrographolide-loaded lipid nanoparticles for enhanced wound healing.
28732886	8	24	theme	tissue	1054:1059	arg1	quality					1061:1067	improved tissue quality	1045:1067	improved tissue quality	1045:1067	The in vivo evaluation in rats reveals that the chitosan-hyaluronic acid/NLC4 sponge enhances the wound healing with no scar and improved tissue quality.
28732886	0	25	theme	composite	25:33	arg1	scaffold					42:49	Chitosan-hyaluronic acid composite sponge scaffold	0:49	Chitosan-hyaluronic acid composite sponge scaffold	0:49	Chitosan-hyaluronic acid composite sponge scaffold enriched with Andrographolide-loaded lipid nanoparticles for enhanced wound healing.
28732886	2	26	theme	factorial	343:351	arg1	design					353:358	23 factorial design	340:358	23 factorial design	340:358	Nanocarriers were prepared using solvent diffusion method by applying 23 factorial design.
28732886	1	27	theme	lipid	236:240	arg1	nanocarriers					242:253	Andrographolide (AND) lipid nanocarriers	214:253	nanocarriers	242:253	In this work chitosan-hyaluronic acid composite sponge scaffold enriched with Andrographolide (AND) lipid nanocarriers was developed.
28732886	7	28	with	porosity	873:880	arg1	swelling					906:913	enhanced swelling	897:913	enhanced swelling	897:913	Nanocomposites showed a porosity of 56.22% with enhanced swelling.
28732886	9	29	theme	wound	1178:1182	arg1	application					1189:1199	wound care application	1178:1199	wound care application	1178:1199	These results strongly support the possibility of using this novel chitosan-hyaluronic acid/NLC4 sponge for wound care application.
28732886	9	30	theme	care	1184:1187	arg1	application					1189:1199	wound care application	1178:1199	wound care application	1178:1199	These results strongly support the possibility of using this novel chitosan-hyaluronic acid/NLC4 sponge for wound care application.
28732886	0	31	theme	Andrographolide-loaded	65:86	arg1	nanoparticles					94:106	Andrographolide-loaded lipid nanoparticles	65:106	Andrographolide-loaded lipid nanoparticles	65:106	Chitosan-hyaluronic acid composite sponge scaffold enriched with Andrographolide-loaded lipid nanoparticles for enhanced wound healing.
28732886	5	32	theme	acid/NLC4	702:710	arg1	sponge					726:731	chitosan-hyaluronic acid/NLC4 nanocomposite sponge	682:731	chitosan-hyaluronic acid/NLC4 nanocomposite sponge	682:731	Consecutively, NLC4 was incorporated in a chitosan-hyaluronic acid gel and lyophilized for 24h to obtain chitosan-hyaluronic acid/NLC4 nanocomposite sponge to enhance AND delivery to wound sites.
28732886	0	33	theme	lipid	88:92	arg1	nanoparticles					94:106	Andrographolide-loaded lipid nanoparticles	65:106	Andrographolide-loaded lipid nanoparticles	65:106	Chitosan-hyaluronic acid composite sponge scaffold enriched with Andrographolide-loaded lipid nanoparticles for enhanced wound healing.
28732886	4	34	dep	48-h	571:574	arg1	up					565:566	up	565:566	up	565:566	Itexhibited spherical shape with 253nm, 83.04% entrapment efficiency and a prolonged release of AND up to 48-h.
28732886	5	35	theme	nanocomposite	712:724	arg1	sponge					726:731	chitosan-hyaluronic acid/NLC4 nanocomposite sponge	682:731	chitosan-hyaluronic acid/NLC4 nanocomposite sponge	682:731	Consecutively, NLC4 was incorporated in a chitosan-hyaluronic acid gel and lyophilized for 24h to obtain chitosan-hyaluronic acid/NLC4 nanocomposite sponge to enhance AND delivery to wound sites.
28732886	8	36	theme	acid/NLC4	984:992	arg1	sponge					994:999	the chitosan-hyaluronic acid/NLC4 sponge	960:999	the chitosan-hyaluronic acid/NLC4 sponge	960:999	The in vivo evaluation in rats reveals that the chitosan-hyaluronic acid/NLC4 sponge enhances the wound healing with no scar and improved tissue quality.
28732886	4	37	theme	AND	561:563	arg1	release					550:556	a prolonged release	538:556	a prolonged release of AND up to 48-h	538:574	Itexhibited spherical shape with 253nm, 83.04% entrapment efficiency and a prolonged release of AND up to 48-h.
28732886	4	37	theme	AND	561:563	arg1	efficiency					523:532	83.04% entrapment efficiency	505:532	83.04% entrapment efficiency	505:532	Itexhibited spherical shape with 253nm, 83.04% entrapment efficiency and a prolonged release of AND up to 48-h.
28732886	4	37	theme	AND	561:563	arg1	253nm					498:502	253nm	498:502	253nm	498:502	Itexhibited spherical shape with 253nm, 83.04% entrapment efficiency and a prolonged release of AND up to 48-h.
28732886	8	38	theme	improved	1045:1052	arg1	quality					1061:1067	improved tissue quality	1045:1067	improved tissue quality	1045:1067	The in vivo evaluation in rats reveals that the chitosan-hyaluronic acid/NLC4 sponge enhances the wound healing with no scar and improved tissue quality.
28732886	2	39	theme	diffusion	311:319	arg1	method					321:326	solvent diffusion method	303:326	solvent diffusion method	303:326	Nanocarriers were prepared using solvent diffusion method by applying 23 factorial design.
28732886	8	40	theme	chitosan-hyaluronic	964:982	arg1	sponge					994:999	the chitosan-hyaluronic acid/NLC4 sponge	960:999	the chitosan-hyaluronic acid/NLC4 sponge	960:999	The in vivo evaluation in rats reveals that the chitosan-hyaluronic acid/NLC4 sponge enhances the wound healing with no scar and improved tissue quality.
28732886	1	41	theme	chitosan-hyaluronic	149:167	arg1	scaffold					191:198	chitosan-hyaluronic acid composite sponge scaffold enriched with Andrographolide (AND) lipid nanocarriers	149:253	chitosan-hyaluronic acid composite sponge scaffold enriched with Andrographolide (AND) lipid nanocarriers	149:253	In this work chitosan-hyaluronic acid composite sponge scaffold enriched with Andrographolide (AND) lipid nanocarriers was developed.
28732886	6	42	theme	sponge	791:796	arg1	morphology					777:786	The morphology	773:786	The morphology of sponge	773:796	The morphology of sponge was characterized by scanning electron microscopy.
28732886	2	43	theme	solvent	303:309	arg1	method					321:326	solvent diffusion method	303:326	solvent diffusion method	303:326	Nanocarriers were prepared using solvent diffusion method by applying 23 factorial design.
28732886	1	44	theme	acid	169:172	arg1	scaffold					191:198	chitosan-hyaluronic acid composite sponge scaffold enriched with Andrographolide (AND) lipid nanocarriers	149:253	chitosan-hyaluronic acid composite sponge scaffold enriched with Andrographolide (AND) lipid nanocarriers	149:253	In this work chitosan-hyaluronic acid composite sponge scaffold enriched with Andrographolide (AND) lipid nanocarriers was developed.
28732886	9	45	theme	novel	1131:1135	arg1	sponge					1167:1172	this novel chitosan-hyaluronic acid/NLC4 sponge	1126:1172	this novel chitosan-hyaluronic acid/NLC4 sponge for wound care application	1126:1199	These results strongly support the possibility of using this novel chitosan-hyaluronic acid/NLC4 sponge for wound care application.
28732886	3	46	theme	highest	374:380	arg1	0.882					402:406	0.882	402:406	0.882	402:406	NLC4 had the highest desirability value (0.882) and therefore, it was chosen as an optimal nanocarrier.
28732886	3	46	theme	highest	374:380	arg1	value					395:399	the highest desirability value	370:399	the highest desirability value (0.882)	370:407	NLC4 had the highest desirability value (0.882) and therefore, it was chosen as an optimal nanocarrier.
28732886	3	47	contain	had	366:368	arg2	0.882					402:406	0.882	402:406	0.882	402:406	NLC4 had the highest desirability value (0.882) and therefore, it was chosen as an optimal nanocarrier.
28732886	3	47	contain	had	366:368	arg2	value					395:399	the highest desirability value	370:399	the highest desirability value (0.882)	370:407	NLC4 had the highest desirability value (0.882) and therefore, it was chosen as an optimal nanocarrier.
28732886	3	47	contain	had	366:368	arg1	NLC4					361:364	NLC4	361:364	NLC4	361:364	NLC4 had the highest desirability value (0.882) and therefore, it was chosen as an optimal nanocarrier.
28732886	1	48	theme	composite	174:182	arg1	scaffold					191:198	chitosan-hyaluronic acid composite sponge scaffold enriched with Andrographolide (AND) lipid nanocarriers	149:253	chitosan-hyaluronic acid composite sponge scaffold enriched with Andrographolide (AND) lipid nanocarriers	149:253	In this work chitosan-hyaluronic acid composite sponge scaffold enriched with Andrographolide (AND) lipid nanocarriers was developed.
28732886	3	49	theme	desirability	382:393	arg1	0.882					402:406	0.882	402:406	0.882	402:406	NLC4 had the highest desirability value (0.882) and therefore, it was chosen as an optimal nanocarrier.
28732886	3	49	theme	desirability	382:393	arg1	value					395:399	the highest desirability value	370:399	the highest desirability value (0.882)	370:407	NLC4 had the highest desirability value (0.882) and therefore, it was chosen as an optimal nanocarrier.
28732886	8	50	from	evaluation	928:937	arg1	rats					942:945	rats	942:945	rats	942:945	The in vivo evaluation in rats reveals that the chitosan-hyaluronic acid/NLC4 sponge enhances the wound healing with no scar and improved tissue quality.
28732886	8	51	theme	wound	1014:1018	arg1	healing					1020:1026	the wound healing	1010:1026	the wound healing with no scar and improved tissue quality	1010:1067	The in vivo evaluation in rats reveals that the chitosan-hyaluronic acid/NLC4 sponge enhances the wound healing with no scar and improved tissue quality.
28732886	1	52	theme	sponge	184:189	arg1	scaffold					191:198	chitosan-hyaluronic acid composite sponge scaffold enriched with Andrographolide (AND) lipid nanocarriers	149:253	chitosan-hyaluronic acid composite sponge scaffold enriched with Andrographolide (AND) lipid nanocarriers	149:253	In this work chitosan-hyaluronic acid composite sponge scaffold enriched with Andrographolide (AND) lipid nanocarriers was developed.
28860056	8	0	dep	%	1019:1019	arg1	%					1056:1056	±8.53%	1051:1056	±8.53%	1051:1056	On the fifth day, the healing rates of CCA composite dressing, gauze, and chitosan were 48.49%±1.07%, 28.02%±6.4%, and 38.97%±8.53%, respectively.
28860056	8	0	dep	%	1019:1019	arg1	%					1025:1025	±1.07%	1020:1025	48.49%±1.07%	1014:1025	On the fifth day, the healing rates of CCA composite dressing, gauze, and chitosan were 48.49%±1.07%, 28.02%±6.4%, and 38.97%±8.53%, respectively.
28860056	4	1	theme	seawater	562:569	arg1	prevention					571:580	seawater prevention	562:580	seawater prevention	562:580	The effects on wound healing and seawater prevention of CCA composite dressing were tested by rat wound model.
28860056	0	2	theme	wound	90:94	arg1	healing					96:102	wound healing	90:102	wound healing	90:102	Preparation of chitosan-collagen-alginate composite dressing and its promoting effects on wound healing.
28860056	9	3	theme	histological	1138:1149	arg1	images					1151:1156	histological images	1138:1156	histological images of CCA composite dressing treated rats	1138:1195	More fibroblast and intact re-epithelialization were observed in histological images of CCA composite dressing treated rats, and the expressions of EGF, bFGF, TGF-β, and CD31 increased significantly.
28860056	7	4	dep	gauze	894:898	arg1	ones					920:923	treated ones	912:923	treated ones	912:923	A higher wound healing ratio was observed in CCA composite dressing treated rats than in gauze or chitosan treated ones.
28860056	8	5	theme	chitosan	1000:1007	arg1	%					1019:1019	48.49%	1014:1019	48.49%±1.07%	1014:1025	On the fifth day, the healing rates of CCA composite dressing, gauze, and chitosan were 48.49%±1.07%, 28.02%±6.4%, and 38.97%±8.53%, respectively.
28860056	8	5	theme	chitosan	1000:1007	arg1	rates					956:960	the healing rates	944:960	the healing rates of CCA composite dressing, gauze, and chitosan	944:1007	On the fifth day, the healing rates of CCA composite dressing, gauze, and chitosan were 48.49%±1.07%, 28.02%±6.4%, and 38.97%±8.53%, respectively.
28860056	7	6	theme	treated	873:879	arg1	rats					881:884	CCA composite dressing treated rats	850:884	CCA composite dressing treated rats than in gauze or chitosan treated ones	850:923	A higher wound healing ratio was observed in CCA composite dressing treated rats than in gauze or chitosan treated ones.
28860056	9	7	located	observed	1126:1133	arg2	fibroblast					1078:1087	More fibroblast	1073:1087	More fibroblast	1073:1087	More fibroblast and intact re-epithelialization were observed in histological images of CCA composite dressing treated rats, and the expressions of EGF, bFGF, TGF-β, and CD31 increased significantly.
28860056	9	7	located	observed	1126:1133	arg2	re-epithelialization					1100:1119	intact re-epithelialization	1093:1119	intact re-epithelialization	1093:1119	More fibroblast and intact re-epithelialization were observed in histological images of CCA composite dressing treated rats, and the expressions of EGF, bFGF, TGF-β, and CD31 increased significantly.
28860056	9	7	located	observed	1126:1133	arg1	images					1151:1156	histological images	1138:1156	histological images of CCA composite dressing treated rats	1138:1195	More fibroblast and intact re-epithelialization were observed in histological images of CCA composite dressing treated rats, and the expressions of EGF, bFGF, TGF-β, and CD31 increased significantly.
28860056	0	8	from	Preparation	0:10	arg1	healing					96:102	wound healing	90:102	wound healing	90:102	Preparation of chitosan-collagen-alginate composite dressing and its promoting effects on wound healing.
28860056	5	9	theme	Preliminary	640:650	arg1	biosecurity					652:662	Preliminary biosecurity	640:662	Preliminary biosecurity	640:662	Preliminary biosecurity was tested by cytotoxicity and hemocompatibility.
28860056	8	10	theme	healing	948:954	arg1	%					1019:1019	48.49%	1014:1019	48.49%±1.07%	1014:1025	On the fifth day, the healing rates of CCA composite dressing, gauze, and chitosan were 48.49%±1.07%, 28.02%±6.4%, and 38.97%±8.53%, respectively.
28860056	8	10	theme	healing	948:954	arg1	rates					956:960	the healing rates	944:960	the healing rates of CCA composite dressing, gauze, and chitosan	944:1007	On the fifth day, the healing rates of CCA composite dressing, gauze, and chitosan were 48.49%±1.07%, 28.02%±6.4%, and 38.97%±8.53%, respectively.
28860056	10	11	theme	composite	1277:1285	arg1	dressing					1287:1294	CCA composite dressing	1273:1294	CCA composite dressing	1273:1294	CCA composite dressing showed no significant cytotoxicity, and favorable hemocompatibility.
28860056	11	12	theme	composite	1398:1406	arg1	dressing					1408:1415	CCA composite dressing	1394:1415	CCA composite dressing	1394:1415	These results suggested that CCA composite dressing could prevent against seawater immersion and promote wound healing while having a good biosecurity.
28860056	7	13	from	rats	881:884	arg1	chitosan					903:910	chitosan	903:910	chitosan	903:910	A higher wound healing ratio was observed in CCA composite dressing treated rats than in gauze or chitosan treated ones.
28860056	7	13	from	rats	881:884	arg1	gauze					894:898	gauze	894:898	gauze	894:898	A higher wound healing ratio was observed in CCA composite dressing treated rats than in gauze or chitosan treated ones.
28860056	8	14	theme	CCA	965:967	arg1	dressing					979:986	CCA composite dressing	965:986	CCA composite dressing	965:986	On the fifth day, the healing rates of CCA composite dressing, gauze, and chitosan were 48.49%±1.07%, 28.02%±6.4%, and 38.97%±8.53%, respectively.
28860056	2	15	theme	Chitosan-collagen-alginate	276:301	arg1	cushion					309:315	Chitosan-collagen-alginate (CCA) cushion	276:315	Chitosan-collagen-alginate (CCA) cushion	276:315	Chitosan-collagen-alginate (CCA) cushion was prepared by paintcoat and freeze-drying, and it was attached to a polyurethane to compose CCA composite dressing.
28860056	7	16	theme	composite	854:862	arg1	rats					881:884	CCA composite dressing treated rats	850:884	CCA composite dressing treated rats than in gauze or chitosan treated ones	850:923	A higher wound healing ratio was observed in CCA composite dressing treated rats than in gauze or chitosan treated ones.
28860056	1	17	theme	seawater	256:263	arg1	immersion					265:273	seawater immersion	256:273	seawater immersion	256:273	The present study aimed to prepare a composite dressing composed of collagen, chitosan, and alginate, which may promote wound healing and prevent from seawater immersion.
28860056	8	18	theme	composite	969:977	arg1	dressing					979:986	CCA composite dressing	965:986	CCA composite dressing	965:986	On the fifth day, the healing rates of CCA composite dressing, gauze, and chitosan were 48.49%±1.07%, 28.02%±6.4%, and 38.97%±8.53%, respectively.
28860056	7	19	theme	dressing	864:871	arg1	rats					881:884	CCA composite dressing treated rats	850:884	CCA composite dressing treated rats than in gauze or chitosan treated ones	850:923	A higher wound healing ratio was observed in CCA composite dressing treated rats than in gauze or chitosan treated ones.
28860056	8	20	theme	dressing	979:986	arg1	%					1019:1019	48.49%	1014:1019	48.49%±1.07%	1014:1025	On the fifth day, the healing rates of CCA composite dressing, gauze, and chitosan were 48.49%±1.07%, 28.02%±6.4%, and 38.97%±8.53%, respectively.
28860056	8	20	theme	dressing	979:986	arg1	rates					956:960	the healing rates	944:960	the healing rates of CCA composite dressing, gauze, and chitosan	944:1007	On the fifth day, the healing rates of CCA composite dressing, gauze, and chitosan were 48.49%±1.07%, 28.02%±6.4%, and 38.97%±8.53%, respectively.
28860056	8	21	from	%	1033:1033	arg1	day					939:941	the fifth day	929:941	the fifth day	929:941	On the fifth day, the healing rates of CCA composite dressing, gauze, and chitosan were 48.49%±1.07%, 28.02%±6.4%, and 38.97%±8.53%, respectively.
28860056	9	22	theme	TGF-β	1232:1236	arg1	expressions					1206:1216	the expressions	1202:1216	the expressions of EGF, bFGF, TGF-β, and CD31	1202:1246	More fibroblast and intact re-epithelialization were observed in histological images of CCA composite dressing treated rats, and the expressions of EGF, bFGF, TGF-β, and CD31 increased significantly.
28860056	6	23	theme	mechanical	782:791	arg1	properties					793:802	mechanical properties	782:802	mechanical properties	782:802	The results revealed that CCA cushion had good water absorption and mechanical properties.
28860056	7	24	theme	CCA	850:852	arg1	rats					881:884	CCA composite dressing treated rats	850:884	CCA composite dressing treated rats than in gauze or chitosan treated ones	850:923	A higher wound healing ratio was observed in CCA composite dressing treated rats than in gauze or chitosan treated ones.
28860056	6	25	contain	had	752:754	arg2	absorption					767:776	good water absorption	756:776	good water absorption	756:776	The results revealed that CCA cushion had good water absorption and mechanical properties.
28860056	6	25	contain	had	752:754	arg2	properties					793:802	mechanical properties	782:802	mechanical properties	782:802	The results revealed that CCA cushion had good water absorption and mechanical properties.
28860056	6	25	contain	had	752:754	arg1	cushion					744:750	CCA cushion	740:750	CCA cushion	740:750	The results revealed that CCA cushion had good water absorption and mechanical properties.
28860056	7	26	theme	higher	807:812	arg1	healing					820:826	A higher wound healing	805:826	A higher wound healing ratio	805:832	A higher wound healing ratio was observed in CCA composite dressing treated rats than in gauze or chitosan treated ones.
28860056	8	27	theme	gauze	989:993	arg1	%					1019:1019	48.49%	1014:1019	48.49%±1.07%	1014:1025	On the fifth day, the healing rates of CCA composite dressing, gauze, and chitosan were 48.49%±1.07%, 28.02%±6.4%, and 38.97%±8.53%, respectively.
28860056	8	27	theme	gauze	989:993	arg1	rates					956:960	the healing rates	944:960	the healing rates of CCA composite dressing, gauze, and chitosan	944:1007	On the fifth day, the healing rates of CCA composite dressing, gauze, and chitosan were 48.49%±1.07%, 28.02%±6.4%, and 38.97%±8.53%, respectively.
28860056	8	28	from	%	1050:1050	arg1	day					939:941	the fifth day	929:941	the fifth day	929:941	On the fifth day, the healing rates of CCA composite dressing, gauze, and chitosan were 48.49%±1.07%, 28.02%±6.4%, and 38.97%±8.53%, respectively.
28860056	0	29	theme	composite	42:50	arg1	dressing					52:59	chitosan-collagen-alginate composite dressing	15:59	chitosan-collagen-alginate composite dressing	15:59	Preparation of chitosan-collagen-alginate composite dressing and its promoting effects on wound healing.
28860056	7	30	theme	wound	814:818	arg1	healing					820:826	A higher wound healing	805:826	A higher wound healing ratio	805:832	A higher wound healing ratio was observed in CCA composite dressing treated rats than in gauze or chitosan treated ones.
28860056	7	31	located	observed	838:845	arg1	rats					881:884	CCA composite dressing treated rats	850:884	CCA composite dressing treated rats than in gauze or chitosan treated ones	850:923	A higher wound healing ratio was observed in CCA composite dressing treated rats than in gauze or chitosan treated ones.
28860056	7	31	located	observed	838:845	arg2	ratio					828:832	A higher wound healing ratio	805:832	A higher wound healing ratio	805:832	A higher wound healing ratio was observed in CCA composite dressing treated rats than in gauze or chitosan treated ones.
28860056	11	32	theme	good	1499:1502	arg1	biosecurity					1504:1514	a good biosecurity	1497:1514	a good biosecurity	1497:1514	These results suggested that CCA composite dressing could prevent against seawater immersion and promote wound healing while having a good biosecurity.
28860056	1	33	theme	present	109:115	arg1	study					117:121	The present study	105:121	The present study	105:121	The present study aimed to prepare a composite dressing composed of collagen, chitosan, and alginate, which may promote wound healing and prevent from seawater immersion.
28860056	10	34	theme	significant	1306:1316	arg1	cytotoxicity					1318:1329	no significant cytotoxicity	1303:1329	no significant cytotoxicity	1303:1329	CCA composite dressing showed no significant cytotoxicity, and favorable hemocompatibility.
28860056	2	35	theme	CCA	411:413	arg1	dressing					425:432	CCA composite dressing	411:432	CCA composite dressing	411:432	Chitosan-collagen-alginate (CCA) cushion was prepared by paintcoat and freeze-drying, and it was attached to a polyurethane to compose CCA composite dressing.
28860056	2	36	theme	composite	415:423	arg1	dressing					425:432	CCA composite dressing	411:432	CCA composite dressing	411:432	Chitosan-collagen-alginate (CCA) cushion was prepared by paintcoat and freeze-drying, and it was attached to a polyurethane to compose CCA composite dressing.
28860056	9	37	theme	CD31	1243:1246	arg1	expressions					1206:1216	the expressions	1202:1216	the expressions of EGF, bFGF, TGF-β, and CD31	1202:1246	More fibroblast and intact re-epithelialization were observed in histological images of CCA composite dressing treated rats, and the expressions of EGF, bFGF, TGF-β, and CD31 increased significantly.
28860056	9	38	theme	dressing	1175:1182	arg1	rats					1192:1195	CCA composite dressing treated rats	1161:1195	CCA composite dressing treated rats	1161:1195	More fibroblast and intact re-epithelialization were observed in histological images of CCA composite dressing treated rats, and the expressions of EGF, bFGF, TGF-β, and CD31 increased significantly.
28860056	2	39	theme	CCA	304:306	arg1	cushion					309:315	Chitosan-collagen-alginate (CCA) cushion	276:315	Chitosan-collagen-alginate (CCA) cushion	276:315	Chitosan-collagen-alginate (CCA) cushion was prepared by paintcoat and freeze-drying, and it was attached to a polyurethane to compose CCA composite dressing.
28860056	7	40	theme	healing	820:826	arg1	ratio					828:832	A higher wound healing ratio	805:832	A higher wound healing ratio	805:832	A higher wound healing ratio was observed in CCA composite dressing treated rats than in gauze or chitosan treated ones.
28860056	1	41	theme	composite	142:150	arg1	dressing					152:159	a composite dressing	140:159	a composite dressing composed of collagen, chitosan, and alginate	140:204	The present study aimed to prepare a composite dressing composed of collagen, chitosan, and alginate, which may promote wound healing and prevent from seawater immersion.
28860056	4	42	theme	wound	544:548	arg1	healing					550:556	wound healing	544:556	wound healing	544:556	The effects on wound healing and seawater prevention of CCA composite dressing were tested by rat wound model.
28860056	9	43	theme	bFGF	1226:1229	arg1	expressions					1206:1216	the expressions	1202:1216	the expressions of EGF, bFGF, TGF-β, and CD31	1202:1246	More fibroblast and intact re-epithelialization were observed in histological images of CCA composite dressing treated rats, and the expressions of EGF, bFGF, TGF-β, and CD31 increased significantly.
28860056	9	44	theme	CCA	1161:1163	arg1	rats					1192:1195	CCA composite dressing treated rats	1161:1195	CCA composite dressing treated rats	1161:1195	More fibroblast and intact re-epithelialization were observed in histological images of CCA composite dressing treated rats, and the expressions of EGF, bFGF, TGF-β, and CD31 increased significantly.
28860056	4	45	theme	wound	627:631	arg1	model					633:637	rat wound model	623:637	rat wound model	623:637	The effects on wound healing and seawater prevention of CCA composite dressing were tested by rat wound model.
28860056	0	46	theme	dressing	52:59	arg1	Preparation					0:10	Preparation	0:10	Preparation of chitosan-collagen-alginate composite dressing	0:59	Preparation of chitosan-collagen-alginate composite dressing and its promoting effects on wound healing.
28860056	0	46	theme	dressing	52:59	arg1	effects					79:85	its promoting effects	65:85	its promoting effects on wound healing	65:102	Preparation of chitosan-collagen-alginate composite dressing and its promoting effects on wound healing.
28860056	8	47	dep	%	1033:1033	arg1	%					1038:1038	±6.4%	1034:1038	28.02%±6.4%	1028:1038	On the fifth day, the healing rates of CCA composite dressing, gauze, and chitosan were 48.49%±1.07%, 28.02%±6.4%, and 38.97%±8.53%, respectively.
28860056	9	48	theme	composite	1165:1173	arg1	rats					1192:1195	CCA composite dressing treated rats	1161:1195	CCA composite dressing treated rats	1161:1195	More fibroblast and intact re-epithelialization were observed in histological images of CCA composite dressing treated rats, and the expressions of EGF, bFGF, TGF-β, and CD31 increased significantly.
28860056	6	49	theme	water	761:765	arg1	absorption					767:776	good water absorption	756:776	good water absorption	756:776	The results revealed that CCA cushion had good water absorption and mechanical properties.
28860056	4	50	theme	rat	623:625	arg1	model					633:637	rat wound model	623:637	rat wound model	623:637	The effects on wound healing and seawater prevention of CCA composite dressing were tested by rat wound model.
28860056	0	51	theme	promoting	69:77	arg1	effects					79:85	its promoting effects	65:85	its promoting effects on wound healing	65:102	Preparation of chitosan-collagen-alginate composite dressing and its promoting effects on wound healing.
28860056	0	52	from	effects	79:85	arg1	healing					96:102	wound healing	90:102	wound healing	90:102	Preparation of chitosan-collagen-alginate composite dressing and its promoting effects on wound healing.
28860056	6	53	theme	good	756:759	arg1	absorption					767:776	good water absorption	756:776	good water absorption	756:776	The results revealed that CCA cushion had good water absorption and mechanical properties.
28860056	11	54	theme	seawater	1439:1446	arg1	immersion					1448:1456	seawater immersion	1439:1456	seawater immersion	1439:1456	These results suggested that CCA composite dressing could prevent against seawater immersion and promote wound healing while having a good biosecurity.
28860056	9	55	theme	EGF	1221:1223	arg1	expressions					1206:1216	the expressions	1202:1216	the expressions of EGF, bFGF, TGF-β, and CD31	1202:1246	More fibroblast and intact re-epithelialization were observed in histological images of CCA composite dressing treated rats, and the expressions of EGF, bFGF, TGF-β, and CD31 increased significantly.
28860056	8	56	from	%	1019:1019	arg1	day					939:941	the fifth day	929:941	the fifth day	929:941	On the fifth day, the healing rates of CCA composite dressing, gauze, and chitosan were 48.49%±1.07%, 28.02%±6.4%, and 38.97%±8.53%, respectively.
28860056	9	57	theme	treated	1184:1190	arg1	rats					1192:1195	CCA composite dressing treated rats	1161:1195	CCA composite dressing treated rats	1161:1195	More fibroblast and intact re-epithelialization were observed in histological images of CCA composite dressing treated rats, and the expressions of EGF, bFGF, TGF-β, and CD31 increased significantly.
28860056	3	58	theme	mechanical	476:485	arg1	properties					487:496	mechanical properties	476:496	mechanical properties	476:496	The swelling, porosity, degradation, and mechanical properties of CCA cushion were evaluated.
28860056	10	59	theme	CCA	1273:1275	arg1	dressing					1287:1294	CCA composite dressing	1273:1294	CCA composite dressing	1273:1294	CCA composite dressing showed no significant cytotoxicity, and favorable hemocompatibility.
28860056	9	60	theme	rats	1192:1195	arg1	images					1151:1156	histological images	1138:1156	histological images of CCA composite dressing treated rats	1138:1195	More fibroblast and intact re-epithelialization were observed in histological images of CCA composite dressing treated rats, and the expressions of EGF, bFGF, TGF-β, and CD31 increased significantly.
28860056	3	61	dep	swelling	439:446	arg1	The					435:437	The	435:437	The	435:437	The swelling, porosity, degradation, and mechanical properties of CCA cushion were evaluated.
28860056	9	62	theme	intact	1093:1098	arg1	re-epithelialization					1100:1119	intact re-epithelialization	1093:1119	intact re-epithelialization	1093:1119	More fibroblast and intact re-epithelialization were observed in histological images of CCA composite dressing treated rats, and the expressions of EGF, bFGF, TGF-β, and CD31 increased significantly.
28860056	10	63	theme	favorable	1336:1344	arg1	hemocompatibility					1346:1362	favorable hemocompatibility	1336:1362	favorable hemocompatibility	1336:1362	CCA composite dressing showed no significant cytotoxicity, and favorable hemocompatibility.
28860056	7	64	theme	treated	912:918	arg1	ones					920:923	treated ones	912:923	treated ones	912:923	A higher wound healing ratio was observed in CCA composite dressing treated rats than in gauze or chitosan treated ones.
28860056	4	65	theme	composite	589:597	arg1	dressing					599:606	CCA composite dressing	585:606	CCA composite dressing	585:606	The effects on wound healing and seawater prevention of CCA composite dressing were tested by rat wound model.
28860056	4	66	from	effects	533:539	arg1	prevention					571:580	seawater prevention	562:580	seawater prevention	562:580	The effects on wound healing and seawater prevention of CCA composite dressing were tested by rat wound model.
28860056	4	66	from	effects	533:539	arg1	healing					550:556	wound healing	544:556	wound healing	544:556	The effects on wound healing and seawater prevention of CCA composite dressing were tested by rat wound model.
28860056	6	67	theme	CCA	740:742	arg1	cushion					744:750	CCA cushion	740:750	CCA cushion	740:750	The results revealed that CCA cushion had good water absorption and mechanical properties.
28860056	11	68	theme	wound	1470:1474	arg1	healing					1476:1482	wound healing	1470:1482	wound healing	1470:1482	These results suggested that CCA composite dressing could prevent against seawater immersion and promote wound healing while having a good biosecurity.
28860056	4	69	theme	CCA	585:587	arg1	dressing					599:606	CCA composite dressing	585:606	CCA composite dressing	585:606	The effects on wound healing and seawater prevention of CCA composite dressing were tested by rat wound model.
28860056	2	70	attach	attached	373:380	arg2	it					366:367	it	366:367	it	366:367	Chitosan-collagen-alginate (CCA) cushion was prepared by paintcoat and freeze-drying, and it was attached to a polyurethane to compose CCA composite dressing.
28860056	2	70	attach	attached	373:380	arg1	polyurethane					387:398	a polyurethane	385:398	a polyurethane	385:398	Chitosan-collagen-alginate (CCA) cushion was prepared by paintcoat and freeze-drying, and it was attached to a polyurethane to compose CCA composite dressing.
28860056	8	71	theme	fifth	933:937	arg1	day					939:941	the fifth day	929:941	the fifth day	929:941	On the fifth day, the healing rates of CCA composite dressing, gauze, and chitosan were 48.49%±1.07%, 28.02%±6.4%, and 38.97%±8.53%, respectively.
28860056	3	72	theme	CCA	501:503	arg1	cushion					505:511	CCA cushion	501:511	CCA cushion	501:511	The swelling, porosity, degradation, and mechanical properties of CCA cushion were evaluated.
28860056	11	73	theme	CCA	1394:1396	arg1	dressing					1408:1415	CCA composite dressing	1394:1415	CCA composite dressing	1394:1415	These results suggested that CCA composite dressing could prevent against seawater immersion and promote wound healing while having a good biosecurity.
28860056	1	74	theme	wound	225:229	arg1	healing					231:237	wound healing	225:237	wound healing	225:237	The present study aimed to prepare a composite dressing composed of collagen, chitosan, and alginate, which may promote wound healing and prevent from seawater immersion.
28860056	0	75	theme	chitosan-collagen-alginate	15:40	arg1	dressing					52:59	chitosan-collagen-alginate composite dressing	15:59	chitosan-collagen-alginate composite dressing	15:59	Preparation of chitosan-collagen-alginate composite dressing and its promoting effects on wound healing.
28860056	9	76	theme	More	1073:1076	arg1	fibroblast					1078:1087	More fibroblast	1073:1087	More fibroblast	1073:1087	More fibroblast and intact re-epithelialization were observed in histological images of CCA composite dressing treated rats, and the expressions of EGF, bFGF, TGF-β, and CD31 increased significantly.
28860056	3	77	theme	cushion	505:511	arg1	degradation					459:469	degradation	459:469	degradation	459:469	The swelling, porosity, degradation, and mechanical properties of CCA cushion were evaluated.
28860056	3	77	theme	cushion	505:511	arg1	porosity					449:456	porosity	449:456	porosity	449:456	The swelling, porosity, degradation, and mechanical properties of CCA cushion were evaluated.
28860056	3	77	theme	cushion	505:511	arg1	properties					487:496	mechanical properties	476:496	mechanical properties	476:496	The swelling, porosity, degradation, and mechanical properties of CCA cushion were evaluated.
28860056	3	77	theme	cushion	505:511	arg1	swelling					439:446	swelling	439:446	swelling	439:446	The swelling, porosity, degradation, and mechanical properties of CCA cushion were evaluated.
28860056	4	78	theme	dressing	599:606	arg1	effects					533:539	The effects	529:539	The effects on wound healing and seawater prevention of CCA composite dressing	529:606	The effects on wound healing and seawater prevention of CCA composite dressing were tested by rat wound model.
26986023	0	0	theme	hybrid	89:94	arg1	chain					96:100	the chondroitin sulfate/dermatan sulfate hybrid chain	48:100	the chondroitin sulfate/dermatan sulfate hybrid chain	48:100	Facile analysis of contents and compositions of the chondroitin sulfate/dermatan sulfate hybrid chain in shark and ray tissues.
26986023	5	1	located	observed	553:560	arg1	contents					569:576	the contents	565:576	the contents of the CS/DS hybrid chain, which were the highest in Mako and Blue sharks and Sharpspine skates, but were lower in Hammerhead sharks	565:709	Species-specific differences were observed in the contents of the CS/DS hybrid chain, which were the highest in Mako and Blue sharks and Sharpspine skates, but were lower in Hammerhead sharks.
26986023	5	1	located	observed	553:560	arg2	differences					536:546	Species-specific differences	519:546	Species-specific differences	519:546	Species-specific differences were observed in the contents of the CS/DS hybrid chain, which were the highest in Mako and Blue sharks and Sharpspine skates, but were lower in Hammerhead sharks.
26986023	2	2	theme	hybrid	314:319	arg1	chain					321:325	the CS/DS hybrid chain	304:325	the CS/DS hybrid chain	304:325	The contents and sulfation patterns of the CS/DS hybrid chain were precisely analyzed by digestion with chondroitinases ABC and AC.
26986023	0	3	theme	sulfate	81:87	arg1	chain					96:100	the chondroitin sulfate/dermatan sulfate hybrid chain	48:100	the chondroitin sulfate/dermatan sulfate hybrid chain	48:100	Facile analysis of contents and compositions of the chondroitin sulfate/dermatan sulfate hybrid chain in shark and ray tissues.
26986023	0	4	from	analysis	7:14	arg1	shark					105:109	shark	105:109	shark	105:109	Facile analysis of contents and compositions of the chondroitin sulfate/dermatan sulfate hybrid chain in shark and ray tissues.
26986023	0	4	from	analysis	7:14	arg1	tissues					119:125	ray tissues	115:125	ray tissues	115:125	Facile analysis of contents and compositions of the chondroitin sulfate/dermatan sulfate hybrid chain in shark and ray tissues.
26986023	7	5	theme	oxidative	899:907	arg1	ability					927:933	an oxidative stress-decreasing ability	896:933	an oxidative stress-decreasing ability	896:933	The contents of the CS/DS hybrid chain and the ratio of the C-unit/A-unit may be related to an oxidative stress-decreasing ability.
26986023	2	6	theme	CS/DS	308:312	arg1	chain					321:325	the CS/DS hybrid chain	304:325	the CS/DS hybrid chain	304:325	The contents and sulfation patterns of the CS/DS hybrid chain were precisely analyzed by digestion with chondroitinases ABC and AC.
26986023	7	7	theme	CS/DS	824:828	arg1	chain					837:841	the CS/DS hybrid chain	820:841	the CS/DS hybrid chain	820:841	The contents of the CS/DS hybrid chain and the ratio of the C-unit/A-unit may be related to an oxidative stress-decreasing ability.
26986023	7	8	theme	stress-decreasing	909:925	arg1	ability					927:933	an oxidative stress-decreasing ability	896:933	an oxidative stress-decreasing ability	896:933	The contents of the CS/DS hybrid chain and the ratio of the C-unit/A-unit may be related to an oxidative stress-decreasing ability.
26986023	5	9	theme	CS/DS	585:589	arg1	lower					684:688	lower	684:688	lower	684:688	Species-specific differences were observed in the contents of the CS/DS hybrid chain, which were the highest in Mako and Blue sharks and Sharpspine skates, but were lower in Hammerhead sharks.
26986023	5	9	theme	CS/DS	585:589	arg1	highest					620:626	highest	620:626	highest	620:626	Species-specific differences were observed in the contents of the CS/DS hybrid chain, which were the highest in Mako and Blue sharks and Sharpspine skates, but were lower in Hammerhead sharks.
26986023	5	9	theme	CS/DS	585:589	arg1	chain					598:602	the CS/DS hybrid chain	581:602	the CS/DS hybrid chain	581:602	Species-specific differences were observed in the contents of the CS/DS hybrid chain, which were the highest in Mako and Blue sharks and Sharpspine skates, but were lower in Hammerhead sharks.
26986023	7	10	theme	hybrid	830:835	arg1	chain					837:841	the CS/DS hybrid chain	820:841	the CS/DS hybrid chain	820:841	The contents of the CS/DS hybrid chain and the ratio of the C-unit/A-unit may be related to an oxidative stress-decreasing ability.
26986023	1	11	theme	several	231:237	arg1	kinds					239:243	several kinds	231:243	several kinds of sharks and rays	231:262	The chondroitin sulfate (CS)/dermatan sulfate (DS) hybrid chain was extracted from specific tissues of several kinds of sharks and rays.
26986023	0	12	theme	chain	96:100	arg1	compositions					32:43	compositions	32:43	compositions	32:43	Facile analysis of contents and compositions of the chondroitin sulfate/dermatan sulfate hybrid chain in shark and ray tissues.
26986023	0	12	theme	chain	96:100	arg1	contents					19:26	contents	19:26	contents	19:26	Facile analysis of contents and compositions of the chondroitin sulfate/dermatan sulfate hybrid chain in shark and ray tissues.
26986023	5	13	theme	hybrid	591:596	arg1	lower					684:688	lower	684:688	lower	684:688	Species-specific differences were observed in the contents of the CS/DS hybrid chain, which were the highest in Mako and Blue sharks and Sharpspine skates, but were lower in Hammerhead sharks.
26986023	5	13	theme	hybrid	591:596	arg1	highest					620:626	highest	620:626	highest	620:626	Species-specific differences were observed in the contents of the CS/DS hybrid chain, which were the highest in Mako and Blue sharks and Sharpspine skates, but were lower in Hammerhead sharks.
26986023	5	13	theme	hybrid	591:596	arg1	chain					598:602	the CS/DS hybrid chain	581:602	the CS/DS hybrid chain	581:602	Species-specific differences were observed in the contents of the CS/DS hybrid chain, which were the highest in Mako and Blue sharks and Sharpspine skates, but were lower in Hammerhead sharks.
26986023	1	14	theme	kinds	239:243	arg1	tissues					220:226	specific tissues	211:226	specific tissues of several kinds of sharks and rays	211:262	The chondroitin sulfate (CS)/dermatan sulfate (DS) hybrid chain was extracted from specific tissues of several kinds of sharks and rays.
26986023	5	15	theme	Hammerhead	693:702	arg1	sharks					704:709	Hammerhead sharks	693:709	Hammerhead sharks	693:709	Species-specific differences were observed in the contents of the CS/DS hybrid chain, which were the highest in Mako and Blue sharks and Sharpspine skates, but were lower in Hammerhead sharks.
26986023	5	16	theme	chain	598:602	arg1	contents					569:576	the contents	565:576	the contents of the CS/DS hybrid chain, which were the highest in Mako and Blue sharks and Sharpspine skates, but were lower in Hammerhead sharks	565:709	Species-specific differences were observed in the contents of the CS/DS hybrid chain, which were the highest in Mako and Blue sharks and Sharpspine skates, but were lower in Hammerhead sharks.
26986023	4	17	contain	contained	497:505	arg1	samples					470:476	all samples	466:476	all samples	466:476	Furthermore, all samples characteristically contained the D-unit.
26986023	4	17	contain	contained	497:505	arg2	D-unit					511:516	the D-unit	507:516	the D-unit	507:516	Furthermore, all samples characteristically contained the D-unit.
26986023	0	18	from	compositions	32:43	arg1	shark					105:109	shark	105:109	shark	105:109	Facile analysis of contents and compositions of the chondroitin sulfate/dermatan sulfate hybrid chain in shark and ray tissues.
26986023	0	18	from	compositions	32:43	arg1	tissues					119:125	ray tissues	115:125	ray tissues	115:125	Facile analysis of contents and compositions of the chondroitin sulfate/dermatan sulfate hybrid chain in shark and ray tissues.
26986023	1	19	theme	/dermatan	156:164	arg1	chain					186:190	The chondroitin sulfate (CS)/dermatan sulfate (DS) hybrid chain	128:190	The chondroitin sulfate (CS)/dermatan sulfate (DS) hybrid chain	128:190	The chondroitin sulfate (CS)/dermatan sulfate (DS) hybrid chain was extracted from specific tissues of several kinds of sharks and rays.
26986023	2	20	with	digestion	354:362	arg1	chondroitinases					369:383	chondroitinases ABC and AC	369:394	chondroitinases ABC and AC	369:394	The contents and sulfation patterns of the CS/DS hybrid chain were precisely analyzed by digestion with chondroitinases ABC and AC.
26986023	2	20	with	digestion	354:362	arg1	AC					393:394	AC	393:394	AC	393:394	The contents and sulfation patterns of the CS/DS hybrid chain were precisely analyzed by digestion with chondroitinases ABC and AC.
26986023	2	20	with	digestion	354:362	arg1	ABC					385:387	ABC	385:387	ABC	385:387	The contents and sulfation patterns of the CS/DS hybrid chain were precisely analyzed by digestion with chondroitinases ABC and AC.
26986023	1	21	theme	sulfate	166:172	arg1	chain					186:190	The chondroitin sulfate (CS)/dermatan sulfate (DS) hybrid chain	128:190	The chondroitin sulfate (CS)/dermatan sulfate (DS) hybrid chain	128:190	The chondroitin sulfate (CS)/dermatan sulfate (DS) hybrid chain was extracted from specific tissues of several kinds of sharks and rays.
26986023	0	22	theme	Facile	0:5	arg1	analysis					7:14	Facile analysis	0:14	Facile analysis of contents and compositions of the chondroitin sulfate/dermatan sulfate hybrid chain in shark and ray tissues.	0:126	Facile analysis of contents and compositions of the chondroitin sulfate/dermatan sulfate hybrid chain in shark and ray tissues.
26986023	7	23	theme	C-unit/A-unit	864:876	arg1	chain					837:841	the CS/DS hybrid chain	820:841	the CS/DS hybrid chain	820:841	The contents of the CS/DS hybrid chain and the ratio of the C-unit/A-unit may be related to an oxidative stress-decreasing ability.
26986023	7	23	theme	C-unit/A-unit	864:876	arg1	ratio					851:855	the ratio	847:855	the ratio of the C-unit/A-unit	847:876	The contents of the CS/DS hybrid chain and the ratio of the C-unit/A-unit may be related to an oxidative stress-decreasing ability.
26986023	0	24	theme	contents	19:26	arg1	analysis					7:14	Facile analysis	0:14	Facile analysis of contents and compositions of the chondroitin sulfate/dermatan sulfate hybrid chain in shark and ray tissues.	0:126	Facile analysis of contents and compositions of the chondroitin sulfate/dermatan sulfate hybrid chain in shark and ray tissues.
26986023	1	25	theme	sharks	248:253	arg1	kinds					239:243	several kinds	231:243	several kinds of sharks and rays	231:262	The chondroitin sulfate (CS)/dermatan sulfate (DS) hybrid chain was extracted from specific tissues of several kinds of sharks and rays.
26986023	1	26	theme	DS	175:176	arg1	chain					186:190	The chondroitin sulfate (CS)/dermatan sulfate (DS) hybrid chain	128:190	The chondroitin sulfate (CS)/dermatan sulfate (DS) hybrid chain	128:190	The chondroitin sulfate (CS)/dermatan sulfate (DS) hybrid chain was extracted from specific tissues of several kinds of sharks and rays.
26986023	0	27	theme	ray	115:117	arg1	tissues					119:125	ray tissues	115:125	ray tissues	115:125	Facile analysis of contents and compositions of the chondroitin sulfate/dermatan sulfate hybrid chain in shark and ray tissues.
26986023	3	28	contain	contained	423:431	arg2	A-					437:438	A-	437:438	A-	437:438	All samples predominantly contained the A- and C-units.
26986023	3	28	contain	contained	423:431	arg1	samples					401:407	All samples	397:407	All samples	397:407	All samples predominantly contained the A- and C-units.
26986023	3	28	contain	contained	423:431	arg2	C-units					444:450	C-units	444:450	C-units	444:450	All samples predominantly contained the A- and C-units.
26986023	2	29	dep	contents	269:276	arg1	The					265:267	The	265:267	The	265:267	The contents and sulfation patterns of the CS/DS hybrid chain were precisely analyzed by digestion with chondroitinases ABC and AC.
26986023	0	30	theme	compositions	32:43	arg1	analysis					7:14	Facile analysis	0:14	Facile analysis of contents and compositions of the chondroitin sulfate/dermatan sulfate hybrid chain in shark and ray tissues.	0:126	Facile analysis of contents and compositions of the chondroitin sulfate/dermatan sulfate hybrid chain in shark and ray tissues.
26986023	1	31	theme	rays	259:262	arg1	kinds					239:243	several kinds	231:243	several kinds of sharks and rays	231:262	The chondroitin sulfate (CS)/dermatan sulfate (DS) hybrid chain was extracted from specific tissues of several kinds of sharks and rays.
26986023	2	32	dep	chondroitinases	369:383	arg1	chondroitinases					369:383	chondroitinases ABC and AC	369:394	chondroitinases ABC and AC	369:394	The contents and sulfation patterns of the CS/DS hybrid chain were precisely analyzed by digestion with chondroitinases ABC and AC.
26986023	2	32	dep	chondroitinases	369:383	arg1	AC					393:394	AC	393:394	AC	393:394	The contents and sulfation patterns of the CS/DS hybrid chain were precisely analyzed by digestion with chondroitinases ABC and AC.
26986023	2	32	dep	chondroitinases	369:383	arg1	ABC					385:387	ABC	385:387	ABC	385:387	The contents and sulfation patterns of the CS/DS hybrid chain were precisely analyzed by digestion with chondroitinases ABC and AC.
26986023	1	33	theme	hybrid	179:184	arg1	chain					186:190	The chondroitin sulfate (CS)/dermatan sulfate (DS) hybrid chain	128:190	The chondroitin sulfate (CS)/dermatan sulfate (DS) hybrid chain	128:190	The chondroitin sulfate (CS)/dermatan sulfate (DS) hybrid chain was extracted from specific tissues of several kinds of sharks and rays.
26986023	5	34	theme	Species-specific	519:534	arg1	differences					536:546	Species-specific differences	519:546	Species-specific differences	519:546	Species-specific differences were observed in the contents of the CS/DS hybrid chain, which were the highest in Mako and Blue sharks and Sharpspine skates, but were lower in Hammerhead sharks.
26986023	5	35	theme	Blue	640:643	arg1	sharks					645:650	sharks	645:650	sharks	645:650	Species-specific differences were observed in the contents of the CS/DS hybrid chain, which were the highest in Mako and Blue sharks and Sharpspine skates, but were lower in Hammerhead sharks.
26986023	2	36	theme	chain	321:325	arg1	contents					269:276	contents	269:276	contents	269:276	The contents and sulfation patterns of the CS/DS hybrid chain were precisely analyzed by digestion with chondroitinases ABC and AC.
26986023	2	36	theme	chain	321:325	arg1	patterns					292:299	sulfation patterns	282:299	sulfation patterns	282:299	The contents and sulfation patterns of the CS/DS hybrid chain were precisely analyzed by digestion with chondroitinases ABC and AC.
26986023	6	37	theme	Marked	712:717	arg1	differences					719:729	Marked differences	712:729	Marked differences	712:729	Marked differences were observed in the ratio of the C-unit/A-unit between sharks and rays.
26986023	0	38	from	shark	105:109	arg1	analysis					7:14	Facile analysis	0:14	Facile analysis of contents and compositions of the chondroitin sulfate/dermatan sulfate hybrid chain in shark and ray tissues.	0:126	Facile analysis of contents and compositions of the chondroitin sulfate/dermatan sulfate hybrid chain in shark and ray tissues.
26986023	6	39	theme	C-unit/A-unit	765:777	arg1	ratio					752:756	the ratio	748:756	the ratio of the C-unit/A-unit between sharks and rays	748:801	Marked differences were observed in the ratio of the C-unit/A-unit between sharks and rays.
26986023	6	40	located	observed	736:743	arg1	ratio					752:756	the ratio	748:756	the ratio of the C-unit/A-unit between sharks and rays	748:801	Marked differences were observed in the ratio of the C-unit/A-unit between sharks and rays.
26986023	6	40	located	observed	736:743	arg2	differences					719:729	Marked differences	712:729	Marked differences	712:729	Marked differences were observed in the ratio of the C-unit/A-unit between sharks and rays.
26986023	5	41	theme	Sharpspine	656:665	arg1	skates					667:672	Sharpspine skates	656:672	Sharpspine skates	656:672	Species-specific differences were observed in the contents of the CS/DS hybrid chain, which were the highest in Mako and Blue sharks and Sharpspine skates, but were lower in Hammerhead sharks.
26986023	2	42	theme	sulfation	282:290	arg1	patterns					292:299	sulfation patterns	282:299	sulfation patterns	282:299	The contents and sulfation patterns of the CS/DS hybrid chain were precisely analyzed by digestion with chondroitinases ABC and AC.
26986023	0	43	theme	sulfate/dermatan	64:79	arg1	chain					96:100	the chondroitin sulfate/dermatan sulfate hybrid chain	48:100	the chondroitin sulfate/dermatan sulfate hybrid chain	48:100	Facile analysis of contents and compositions of the chondroitin sulfate/dermatan sulfate hybrid chain in shark and ray tissues.
26986023	1	44	theme	chondroitin	132:142	arg1	sulfate					144:150	chondroitin sulfate	132:150	The chondroitin sulfate (CS)/dermatan sulfate (DS) hybrid chain	128:190	The chondroitin sulfate (CS)/dermatan sulfate (DS) hybrid chain was extracted from specific tissues of several kinds of sharks and rays.
26986023	1	44	theme	chondroitin	132:142	arg1	CS					153:154	CS	153:154	CS	153:154	The chondroitin sulfate (CS)/dermatan sulfate (DS) hybrid chain was extracted from specific tissues of several kinds of sharks and rays.
26986023	7	45	theme	ratio	851:855	arg1	contents					808:815	The contents	804:815	The contents of the CS/DS hybrid chain and the ratio of the C-unit/A-unit	804:876	The contents of the CS/DS hybrid chain and the ratio of the C-unit/A-unit may be related to an oxidative stress-decreasing ability.
26986023	7	45	theme	ratio	851:855	arg1	related					885:891	related	885:891	related	885:891	The contents of the CS/DS hybrid chain and the ratio of the C-unit/A-unit may be related to an oxidative stress-decreasing ability.
26986023	0	46	from	tissues	119:125	arg1	analysis					7:14	Facile analysis	0:14	Facile analysis of contents and compositions of the chondroitin sulfate/dermatan sulfate hybrid chain in shark and ray tissues.	0:126	Facile analysis of contents and compositions of the chondroitin sulfate/dermatan sulfate hybrid chain in shark and ray tissues.
26986023	0	47	theme	chondroitin	52:62	arg1	chain					96:100	the chondroitin sulfate/dermatan sulfate hybrid chain	48:100	the chondroitin sulfate/dermatan sulfate hybrid chain	48:100	Facile analysis of contents and compositions of the chondroitin sulfate/dermatan sulfate hybrid chain in shark and ray tissues.
26986023	0	48	from	contents	19:26	arg1	shark					105:109	shark	105:109	shark	105:109	Facile analysis of contents and compositions of the chondroitin sulfate/dermatan sulfate hybrid chain in shark and ray tissues.
26986023	0	48	from	contents	19:26	arg1	tissues					119:125	ray tissues	115:125	ray tissues	115:125	Facile analysis of contents and compositions of the chondroitin sulfate/dermatan sulfate hybrid chain in shark and ray tissues.
26986023	1	49	theme	sulfate	144:150	arg1	chain					186:190	The chondroitin sulfate (CS)/dermatan sulfate (DS) hybrid chain	128:190	The chondroitin sulfate (CS)/dermatan sulfate (DS) hybrid chain	128:190	The chondroitin sulfate (CS)/dermatan sulfate (DS) hybrid chain was extracted from specific tissues of several kinds of sharks and rays.
26986023	7	50	theme	chain	837:841	arg1	contents					808:815	The contents	804:815	The contents of the CS/DS hybrid chain and the ratio of the C-unit/A-unit	804:876	The contents of the CS/DS hybrid chain and the ratio of the C-unit/A-unit may be related to an oxidative stress-decreasing ability.
26986023	7	50	theme	chain	837:841	arg1	related					885:891	related	885:891	related	885:891	The contents of the CS/DS hybrid chain and the ratio of the C-unit/A-unit may be related to an oxidative stress-decreasing ability.
26986023	1	51	theme	specific	211:218	arg1	tissues					220:226	specific tissues	211:226	specific tissues of several kinds of sharks and rays	211:262	The chondroitin sulfate (CS)/dermatan sulfate (DS) hybrid chain was extracted from specific tissues of several kinds of sharks and rays.
26829372	0	0	theme	Two-Dimensional	64:78	arg1	Systems					80:86	Two-Dimensional Systems	64:86	Two-Dimensional Systems	64:86	Mimicking the Humidity Response of the Plant Cell Wall by Using Two-Dimensional Systems: The Critical Role of Amorphous and Crystalline Polysaccharides.
26829372	1	1	theme	microfibrils	315:326	arg1	arrangement					274:284	a complex arrangement	264:284	a complex arrangement of semicrystalline cellulose microfibrils in a dissipative matrix of lignin and hemicelluloses	264:379	Of the composite materials occurring in nature, the plant cell wall is among the most intricate, consisting of a complex arrangement of semicrystalline cellulose microfibrils in a dissipative matrix of lignin and hemicelluloses.
26829372	4	2	theme	Quantitative	714:725	arg1	analysis					727:734	Quantitative analysis	714:734	Quantitative analysis	714:734	Quantitative analysis shows that the vapor-induced swelling of these ultrathin films can be controlled by varying ratios of the chemically identical ordered and unordered cellulose components.
26829372	5	3	theme	amorphous	1142:1150	arg1	overlayer					1152:1160	an amorphous overlayer	1139:1160	an amorphous overlayer	1139:1160	Intriguingly, increasing the share of crystalline cellulose appeared to increase the vapor uptake but only in cases for which the interfacial area between the crystalline and amorphous area was relatively large and the thickness of an amorphous overlayer was relatively small.
26829372	3	4	used	used	647:650	arg2	QCM-D					637:641	QCM-D	637:641	QCM-D	637:641	Spectroscopic ellipsometry and QCM-D are used to study the water vapor uptake of several two-layer systems.
26829372	3	4	used	used	647:650	arg2	ellipsometry					620:631	Spectroscopic ellipsometry	606:631	Spectroscopic ellipsometry	606:631	Spectroscopic ellipsometry and QCM-D are used to study the water vapor uptake of several two-layer systems.
26829372	3	5	theme	two-layer	695:703	arg1	systems					705:711	several two-layer systems	687:711	several two-layer systems	687:711	Spectroscopic ellipsometry and QCM-D are used to study the water vapor uptake of several two-layer systems.
26829372	4	6	theme	components	895:904	arg1	ratios					828:833	varying ratios	820:833	varying ratios of the chemically identical ordered and unordered cellulose components	820:904	Quantitative analysis shows that the vapor-induced swelling of these ultrathin films can be controlled by varying ratios of the chemically identical ordered and unordered cellulose components.
26829372	2	7	theme	crystalline	517:527	arg1	portion					529:535	the crystalline portion	513:535	the crystalline portion	513:535	Here, a biomimetic, two-dimensional cellulose system of the cell wall structure is introduced where cellulose nanocrystals compose the crystalline portion and regenerated amorphous cellulose composes the dissipative matrix.
26829372	3	8	theme	systems	705:711	arg1	uptake					677:682	the water vapor uptake	661:682	the water vapor uptake of several two-layer systems	661:711	Spectroscopic ellipsometry and QCM-D are used to study the water vapor uptake of several two-layer systems.
26829372	5	9	theme	overlayer	1152:1160	arg1	thickness					1126:1134	the thickness	1122:1134	the thickness of an amorphous overlayer	1122:1160	Intriguingly, increasing the share of crystalline cellulose appeared to increase the vapor uptake but only in cases for which the interfacial area between the crystalline and amorphous area was relatively large and the thickness of an amorphous overlayer was relatively small.
26829372	5	9	theme	overlayer	1152:1160	arg1	small					1177:1181	small	1177:1181	small	1177:1181	Intriguingly, increasing the share of crystalline cellulose appeared to increase the vapor uptake but only in cases for which the interfacial area between the crystalline and amorphous area was relatively large and the thickness of an amorphous overlayer was relatively small.
26829372	4	10	theme	films	793:797	arg1	swelling					765:772	the vapor-induced swelling	747:772	the vapor-induced swelling of these ultrathin films	747:797	Quantitative analysis shows that the vapor-induced swelling of these ultrathin films can be controlled by varying ratios of the chemically identical ordered and unordered cellulose components.
26829372	2	11	dep	portion	529:535	arg1	matrix					598:603	the dissipative matrix	582:603	the dissipative matrix	582:603	Here, a biomimetic, two-dimensional cellulose system of the cell wall structure is introduced where cellulose nanocrystals compose the crystalline portion and regenerated amorphous cellulose composes the dissipative matrix.
26829372	1	12	theme	dissipative	333:343	arg1	matrix					345:350	a dissipative matrix	331:350	a dissipative matrix of lignin and hemicelluloses	331:379	Of the composite materials occurring in nature, the plant cell wall is among the most intricate, consisting of a complex arrangement of semicrystalline cellulose microfibrils in a dissipative matrix of lignin and hemicelluloses.
26829372	1	13	from	arrangement	274:284	arg1	matrix					345:350	a dissipative matrix	331:350	a dissipative matrix of lignin and hemicelluloses	331:379	Of the composite materials occurring in nature, the plant cell wall is among the most intricate, consisting of a complex arrangement of semicrystalline cellulose microfibrils in a dissipative matrix of lignin and hemicelluloses.
26829372	4	14	theme	ultrathin	783:791	arg1	films					793:797	these ultrathin films	777:797	these ultrathin films	777:797	Quantitative analysis shows that the vapor-induced swelling of these ultrathin films can be controlled by varying ratios of the chemically identical ordered and unordered cellulose components.
26829372	0	15	theme	Critical	93:100	arg1	Role					102:105	The Critical Role	89:105	The Critical Role of Amorphous and Crystalline Polysaccharides	89:150	Mimicking the Humidity Response of the Plant Cell Wall by Using Two-Dimensional Systems: The Critical Role of Amorphous and Crystalline Polysaccharides.
26829372	6	16	theme	native	1279:1284	arg1	structures					1286:1295	certain native structures	1271:1295	certain native structures	1271:1295	The results show that a biomimetic approach may occasionally provide answers as to why certain native structures exist.
26829372	1	17	from	microfibrils	315:326	arg1	matrix					345:350	a dissipative matrix	331:350	a dissipative matrix of lignin and hemicelluloses	331:379	Of the composite materials occurring in nature, the plant cell wall is among the most intricate, consisting of a complex arrangement of semicrystalline cellulose microfibrils in a dissipative matrix of lignin and hemicelluloses.
26829372	4	18	theme	ordered	863:869	arg1	components					895:904	the chemically identical ordered and unordered cellulose components	838:904	the chemically identical ordered and unordered cellulose components	838:904	Quantitative analysis shows that the vapor-induced swelling of these ultrathin films can be controlled by varying ratios of the chemically identical ordered and unordered cellulose components.
26829372	1	19	theme	composite	160:168	arg1	materials					170:178	the composite materials	156:178	the composite materials occurring in nature	156:198	Of the composite materials occurring in nature, the plant cell wall is among the most intricate, consisting of a complex arrangement of semicrystalline cellulose microfibrils in a dissipative matrix of lignin and hemicelluloses.
26829372	2	20	theme	wall	447:450	arg1	structure					452:460	the cell wall structure	438:460	the cell wall structure	438:460	Here, a biomimetic, two-dimensional cellulose system of the cell wall structure is introduced where cellulose nanocrystals compose the crystalline portion and regenerated amorphous cellulose composes the dissipative matrix.
26829372	1	21	theme	lignin	355:360	arg1	matrix					345:350	a dissipative matrix	331:350	a dissipative matrix of lignin and hemicelluloses	331:379	Of the composite materials occurring in nature, the plant cell wall is among the most intricate, consisting of a complex arrangement of semicrystalline cellulose microfibrils in a dissipative matrix of lignin and hemicelluloses.
26829372	4	22	theme	cellulose	885:893	arg1	components					895:904	the chemically identical ordered and unordered cellulose components	838:904	the chemically identical ordered and unordered cellulose components	838:904	Quantitative analysis shows that the vapor-induced swelling of these ultrathin films can be controlled by varying ratios of the chemically identical ordered and unordered cellulose components.
26829372	4	23	dep	ordered	863:869	arg1	identical					853:861	identical	853:861	identical	853:861	Quantitative analysis shows that the vapor-induced swelling of these ultrathin films can be controlled by varying ratios of the chemically identical ordered and unordered cellulose components.
26829372	1	24	theme	materials	170:178	arg1	intricate					239:247	intricate	239:247	intricate	239:247	Of the composite materials occurring in nature, the plant cell wall is among the most intricate, consisting of a complex arrangement of semicrystalline cellulose microfibrils in a dissipative matrix of lignin and hemicelluloses.
26829372	4	25	theme	unordered	875:883	arg1	components					895:904	the chemically identical ordered and unordered cellulose components	838:904	the chemically identical ordered and unordered cellulose components	838:904	Quantitative analysis shows that the vapor-induced swelling of these ultrathin films can be controlled by varying ratios of the chemically identical ordered and unordered cellulose components.
26829372	4	26	theme	vapor-induced	751:763	arg1	swelling					765:772	the vapor-induced swelling	747:772	the vapor-induced swelling of these ultrathin films	747:797	Quantitative analysis shows that the vapor-induced swelling of these ultrathin films can be controlled by varying ratios of the chemically identical ordered and unordered cellulose components.
26829372	3	27	theme	water	665:669	arg1	uptake					677:682	the water vapor uptake	661:682	the water vapor uptake of several two-layer systems	661:711	Spectroscopic ellipsometry and QCM-D are used to study the water vapor uptake of several two-layer systems.
26829372	2	28	theme	cellulose	418:426	arg1	system					428:433	a biomimetic, two-dimensional cellulose system	388:433	a biomimetic, two-dimensional cellulose system of the cell wall structure	388:460	Here, a biomimetic, two-dimensional cellulose system of the cell wall structure is introduced where cellulose nanocrystals compose the crystalline portion and regenerated amorphous cellulose composes the dissipative matrix.
26829372	2	29	theme	amorphous	553:561	arg1	composes					573:580	regenerated amorphous cellulose composes	541:580	regenerated amorphous cellulose composes	541:580	Here, a biomimetic, two-dimensional cellulose system of the cell wall structure is introduced where cellulose nanocrystals compose the crystalline portion and regenerated amorphous cellulose composes the dissipative matrix.
26829372	5	30	theme	vapor	992:996	arg1	uptake					998:1003	the vapor uptake	988:1003	the vapor uptake	988:1003	Intriguingly, increasing the share of crystalline cellulose appeared to increase the vapor uptake but only in cases for which the interfacial area between the crystalline and amorphous area was relatively large and the thickness of an amorphous overlayer was relatively small.
26829372	6	31	theme	certain	1271:1277	arg1	structures					1286:1295	certain native structures	1271:1295	certain native structures	1271:1295	The results show that a biomimetic approach may occasionally provide answers as to why certain native structures exist.
26829372	1	32	from	matrix	345:350	arg1	arrangement					274:284	a complex arrangement	264:284	a complex arrangement of semicrystalline cellulose microfibrils in a dissipative matrix of lignin and hemicelluloses	264:379	Of the composite materials occurring in nature, the plant cell wall is among the most intricate, consisting of a complex arrangement of semicrystalline cellulose microfibrils in a dissipative matrix of lignin and hemicelluloses.
26829372	2	33	theme	regenerated	541:551	arg1	composes					573:580	regenerated amorphous cellulose composes	541:580	regenerated amorphous cellulose composes	541:580	Here, a biomimetic, two-dimensional cellulose system of the cell wall structure is introduced where cellulose nanocrystals compose the crystalline portion and regenerated amorphous cellulose composes the dissipative matrix.
26829372	2	34	theme	cell	442:445	arg1	structure					452:460	the cell wall structure	438:460	the cell wall structure	438:460	Here, a biomimetic, two-dimensional cellulose system of the cell wall structure is introduced where cellulose nanocrystals compose the crystalline portion and regenerated amorphous cellulose composes the dissipative matrix.
26829372	2	35	theme	biomimetic	390:399	arg1	system					428:433	a biomimetic, two-dimensional cellulose system	388:433	a biomimetic, two-dimensional cellulose system of the cell wall structure	388:460	Here, a biomimetic, two-dimensional cellulose system of the cell wall structure is introduced where cellulose nanocrystals compose the crystalline portion and regenerated amorphous cellulose composes the dissipative matrix.
26829372	2	36	theme	structure	452:460	arg1	system					428:433	a biomimetic, two-dimensional cellulose system	388:433	a biomimetic, two-dimensional cellulose system of the cell wall structure	388:460	Here, a biomimetic, two-dimensional cellulose system of the cell wall structure is introduced where cellulose nanocrystals compose the crystalline portion and regenerated amorphous cellulose composes the dissipative matrix.
26829372	5	37	theme	interfacial	1037:1047	arg1	large					1112:1116	large	1112:1116	large	1112:1116	Intriguingly, increasing the share of crystalline cellulose appeared to increase the vapor uptake but only in cases for which the interfacial area between the crystalline and amorphous area was relatively large and the thickness of an amorphous overlayer was relatively small.
26829372	5	37	theme	interfacial	1037:1047	arg1	area					1049:1052	the interfacial area	1033:1052	the interfacial area between the crystalline and amorphous area	1033:1095	Intriguingly, increasing the share of crystalline cellulose appeared to increase the vapor uptake but only in cases for which the interfacial area between the crystalline and amorphous area was relatively large and the thickness of an amorphous overlayer was relatively small.
26829372	1	38	theme	hemicelluloses	366:379	arg1	matrix					345:350	a dissipative matrix	331:350	a dissipative matrix of lignin and hemicelluloses	331:379	Of the composite materials occurring in nature, the plant cell wall is among the most intricate, consisting of a complex arrangement of semicrystalline cellulose microfibrils in a dissipative matrix of lignin and hemicelluloses.
26829372	3	39	theme	Spectroscopic	606:618	arg1	ellipsometry					620:631	Spectroscopic ellipsometry	606:631	Spectroscopic ellipsometry	606:631	Spectroscopic ellipsometry and QCM-D are used to study the water vapor uptake of several two-layer systems.
26829372	0	40	theme	Humidity	14:21	arg1	Response					23:30	the Humidity Response	10:30	the Humidity Response of the Plant Cell Wall	10:53	Mimicking the Humidity Response of the Plant Cell Wall by Using Two-Dimensional Systems: The Critical Role of Amorphous and Crystalline Polysaccharides.
26829372	1	41	theme	complex	266:272	arg1	arrangement					274:284	a complex arrangement	264:284	a complex arrangement of semicrystalline cellulose microfibrils in a dissipative matrix of lignin and hemicelluloses	264:379	Of the composite materials occurring in nature, the plant cell wall is among the most intricate, consisting of a complex arrangement of semicrystalline cellulose microfibrils in a dissipative matrix of lignin and hemicelluloses.
26829372	0	42	theme	Amorphous	110:118	arg1	Role					102:105	The Critical Role	89:105	The Critical Role of Amorphous and Crystalline Polysaccharides	89:150	Mimicking the Humidity Response of the Plant Cell Wall by Using Two-Dimensional Systems: The Critical Role of Amorphous and Crystalline Polysaccharides.
26829372	0	43	theme	Cell	45:48	arg1	Wall					50:53	the Plant Cell Wall	35:53	the Plant Cell Wall	35:53	Mimicking the Humidity Response of the Plant Cell Wall by Using Two-Dimensional Systems: The Critical Role of Amorphous and Crystalline Polysaccharides.
26829372	2	44	theme	dissipative	586:596	arg1	matrix					598:603	the dissipative matrix	582:603	the dissipative matrix	582:603	Here, a biomimetic, two-dimensional cellulose system of the cell wall structure is introduced where cellulose nanocrystals compose the crystalline portion and regenerated amorphous cellulose composes the dissipative matrix.
26829372	5	45	theme	crystalline	945:955	arg1	cellulose					957:965	crystalline cellulose	945:965	crystalline cellulose	945:965	Intriguingly, increasing the share of crystalline cellulose appeared to increase the vapor uptake but only in cases for which the interfacial area between the crystalline and amorphous area was relatively large and the thickness of an amorphous overlayer was relatively small.
26829372	0	46	theme	Plant	39:43	arg1	Wall					50:53	the Plant Cell Wall	35:53	the Plant Cell Wall	35:53	Mimicking the Humidity Response of the Plant Cell Wall by Using Two-Dimensional Systems: The Critical Role of Amorphous and Crystalline Polysaccharides.
26829372	6	47	theme	biomimetic	1208:1217	arg1	approach					1219:1226	a biomimetic approach	1206:1226	a biomimetic approach	1206:1226	The results show that a biomimetic approach may occasionally provide answers as to why certain native structures exist.
26829372	0	48	theme	Crystalline	124:134	arg1	Role					102:105	The Critical Role	89:105	The Critical Role of Amorphous and Crystalline Polysaccharides	89:150	Mimicking the Humidity Response of the Plant Cell Wall by Using Two-Dimensional Systems: The Critical Role of Amorphous and Crystalline Polysaccharides.
26829372	2	49	dep	biomimetic	390:399	arg1	two-dimensional					402:416	two-dimensional	402:416	two-dimensional	402:416	Here, a biomimetic, two-dimensional cellulose system of the cell wall structure is introduced where cellulose nanocrystals compose the crystalline portion and regenerated amorphous cellulose composes the dissipative matrix.
26829372	5	50	theme	cellulose	957:965	arg1	share					936:940	the share	932:940	the share of crystalline cellulose	932:965	Intriguingly, increasing the share of crystalline cellulose appeared to increase the vapor uptake but only in cases for which the interfacial area between the crystalline and amorphous area was relatively large and the thickness of an amorphous overlayer was relatively small.
26829372	1	51	theme	semicrystalline	289:303	arg1	microfibrils					315:326	semicrystalline cellulose microfibrils	289:326	semicrystalline cellulose microfibrils in a dissipative matrix of lignin and hemicelluloses	289:379	Of the composite materials occurring in nature, the plant cell wall is among the most intricate, consisting of a complex arrangement of semicrystalline cellulose microfibrils in a dissipative matrix of lignin and hemicelluloses.
26829372	3	52	theme	several	687:693	arg1	systems					705:711	several two-layer systems	687:711	several two-layer systems	687:711	Spectroscopic ellipsometry and QCM-D are used to study the water vapor uptake of several two-layer systems.
26829372	1	53	theme	plant	205:209	arg1	wall					216:219	the plant cell wall	201:219	the plant cell wall	201:219	Of the composite materials occurring in nature, the plant cell wall is among the most intricate, consisting of a complex arrangement of semicrystalline cellulose microfibrils in a dissipative matrix of lignin and hemicelluloses.
26829372	2	54	theme	cellulose	563:571	arg1	composes					573:580	regenerated amorphous cellulose composes	541:580	regenerated amorphous cellulose composes	541:580	Here, a biomimetic, two-dimensional cellulose system of the cell wall structure is introduced where cellulose nanocrystals compose the crystalline portion and regenerated amorphous cellulose composes the dissipative matrix.
26829372	3	55	theme	vapor	671:675	arg1	uptake					677:682	the water vapor uptake	661:682	the water vapor uptake of several two-layer systems	661:711	Spectroscopic ellipsometry and QCM-D are used to study the water vapor uptake of several two-layer systems.
26829372	1	56	theme	cell	211:214	arg1	wall					216:219	the plant cell wall	201:219	the plant cell wall	201:219	Of the composite materials occurring in nature, the plant cell wall is among the most intricate, consisting of a complex arrangement of semicrystalline cellulose microfibrils in a dissipative matrix of lignin and hemicelluloses.
26829372	2	57	theme	cellulose	482:490	arg1	nanocrystals					492:503	cellulose nanocrystals	482:503	cellulose nanocrystals	482:503	Here, a biomimetic, two-dimensional cellulose system of the cell wall structure is introduced where cellulose nanocrystals compose the crystalline portion and regenerated amorphous cellulose composes the dissipative matrix.
26829372	4	58	theme	varying	820:826	arg1	ratios					828:833	varying ratios	820:833	varying ratios of the chemically identical ordered and unordered cellulose components	820:904	Quantitative analysis shows that the vapor-induced swelling of these ultrathin films can be controlled by varying ratios of the chemically identical ordered and unordered cellulose components.
26829372	5	59	dep	crystalline	1066:1076	arg1	area					1092:1095	area	1092:1095	area	1092:1095	Intriguingly, increasing the share of crystalline cellulose appeared to increase the vapor uptake but only in cases for which the interfacial area between the crystalline and amorphous area was relatively large and the thickness of an amorphous overlayer was relatively small.
26829372	5	59	dep	crystalline	1066:1076	arg1	the					1062:1064	the	1062:1064	the	1062:1064	Intriguingly, increasing the share of crystalline cellulose appeared to increase the vapor uptake but only in cases for which the interfacial area between the crystalline and amorphous area was relatively large and the thickness of an amorphous overlayer was relatively small.
26829372	0	60	theme	Wall	50:53	arg1	Response					23:30	the Humidity Response	10:30	the Humidity Response of the Plant Cell Wall	10:53	Mimicking the Humidity Response of the Plant Cell Wall by Using Two-Dimensional Systems: The Critical Role of Amorphous and Crystalline Polysaccharides.
26829372	1	61	theme	cellulose	305:313	arg1	microfibrils					315:326	semicrystalline cellulose microfibrils	289:326	semicrystalline cellulose microfibrils in a dissipative matrix of lignin and hemicelluloses	289:379	Of the composite materials occurring in nature, the plant cell wall is among the most intricate, consisting of a complex arrangement of semicrystalline cellulose microfibrils in a dissipative matrix of lignin and hemicelluloses.
26829372	0	62	dep	Mimicking	0:8	arg1	Role					102:105	The Critical Role	89:105	The Critical Role of Amorphous and Crystalline Polysaccharides	89:150	Mimicking the Humidity Response of the Plant Cell Wall by Using Two-Dimensional Systems: The Critical Role of Amorphous and Crystalline Polysaccharides.
26829372	0	63	dep	Amorphous	110:118	arg1	Polysaccharides					136:150	Polysaccharides	136:150	Polysaccharides	136:150	Mimicking the Humidity Response of the Plant Cell Wall by Using Two-Dimensional Systems: The Critical Role of Amorphous and Crystalline Polysaccharides.
25542790	0	0	theme	carboxymethyl	29:41	arg1	derivatives					50:60	carboxymethyl chitin derivatives	29:60	carboxymethyl chitin derivatives	29:60	Biological adhesive based on carboxymethyl chitin derivatives and chitin nanofibers.
25542790	5	1	located	observed	820:827	arg1	HMA-CM-chitin/S-DACNF					854:874	HMA-CM-chitin/S-DACNF	854:874	HMA-CM-chitin/S-DACNF	854:874	Moreover, quick adhesion and induction of inflammatory cells migration were observed in HMA-CM-chitin/CNF and HMA-CM-chitin/S-DACNF.
25542790	5	1	located	observed	820:827	arg2	induction					773:781	induction	773:781	induction of inflammatory cells	773:803	Moreover, quick adhesion and induction of inflammatory cells migration were observed in HMA-CM-chitin/CNF and HMA-CM-chitin/S-DACNF.
25542790	5	1	located	observed	820:827	arg1	HMA-CM-chitin/CNF					832:848	HMA-CM-chitin/CNF	832:848	HMA-CM-chitin/CNF	832:848	Moreover, quick adhesion and induction of inflammatory cells migration were observed in HMA-CM-chitin/CNF and HMA-CM-chitin/S-DACNF.
25542790	5	1	located	observed	820:827	arg2	adhesion					760:767	quick adhesion	754:767	quick adhesion	754:767	Moreover, quick adhesion and induction of inflammatory cells migration were observed in HMA-CM-chitin/CNF and HMA-CM-chitin/S-DACNF.
25542790	2	2	theme	aqueous	394:400	arg1	peroxide					411:418	an aqueous hydrogen peroxide	391:418	an aqueous hydrogen peroxide solution	391:427	Chitin derivatives with acrylic groups, such as 2-hydroxy-3-methacryloyloxypropylated carboxymethyl chitin (HMA-CM-chitin), were synthesized and cured by the addition of an aqueous hydrogen peroxide solution as a radical initiator.
25542790	2	3	theme	radical	434:440	arg1	derivatives					228:238	Chitin derivatives	221:238	Chitin derivatives	221:238	Chitin derivatives with acrylic groups, such as 2-hydroxy-3-methacryloyloxypropylated carboxymethyl chitin (HMA-CM-chitin), were synthesized and cured by the addition of an aqueous hydrogen peroxide solution as a radical initiator.
25542790	2	3	theme	radical	434:440	arg1	initiator					442:450	a radical initiator	432:450	a radical initiator	432:450	Chitin derivatives with acrylic groups, such as 2-hydroxy-3-methacryloyloxypropylated carboxymethyl chitin (HMA-CM-chitin), were synthesized and cured by the addition of an aqueous hydrogen peroxide solution as a radical initiator.
25542790	2	3	theme	radical	434:440	arg1	chitin					321:326	2-hydroxy-3-methacryloyloxypropylated carboxymethyl chitin	269:326	2-hydroxy-3-methacryloyloxypropylated carboxymethyl chitin (HMA-CM-chitin)	269:342	Chitin derivatives with acrylic groups, such as 2-hydroxy-3-methacryloyloxypropylated carboxymethyl chitin (HMA-CM-chitin), were synthesized and cured by the addition of an aqueous hydrogen peroxide solution as a radical initiator.
25542790	2	4	with	derivatives	228:238	arg1	groups					253:258	acrylic groups	245:258	acrylic groups	245:258	Chitin derivatives with acrylic groups, such as 2-hydroxy-3-methacryloyloxypropylated carboxymethyl chitin (HMA-CM-chitin), were synthesized and cured by the addition of an aqueous hydrogen peroxide solution as a radical initiator.
25542790	5	5	dep	adhesion	760:767	arg1	migration					805:813	migration	805:813	migration	805:813	Moreover, quick adhesion and induction of inflammatory cells migration were observed in HMA-CM-chitin/CNF and HMA-CM-chitin/S-DACNF.
25542790	4	6	theme	adhesive	734:741	arg1	cyanoacrylate					720:732	a commercial cyanoacrylate adhesive	707:741	a commercial cyanoacrylate adhesive	707:741	HMA-CM-chitin/CNFs or HMA-CM-chitin/S-DACNFs have almost equal adhesive strength compared to that of a commercial cyanoacrylate adhesive.
25542790	4	7	theme	equal	663:667	arg1	strength					678:685	almost equal adhesive strength	656:685	almost equal adhesive strength	656:685	HMA-CM-chitin/CNFs or HMA-CM-chitin/S-DACNFs have almost equal adhesive strength compared to that of a commercial cyanoacrylate adhesive.
25542790	3	8	theme	chitin	576:581	arg1	S-DACNFs					595:602	S-DACNFs	595:602	S-DACNFs	595:602	The adhesive strength of HMA-CM-chitin increased when it was blended with chitin nanofibers (CNFs) or surface-deacetylated chitin nanofibers (S-DACNFs).
25542790	3	8	theme	chitin	576:581	arg1	nanofibers					583:592	surface-deacetylated chitin nanofibers	555:592	surface-deacetylated chitin nanofibers (S-DACNFs)	555:603	The adhesive strength of HMA-CM-chitin increased when it was blended with chitin nanofibers (CNFs) or surface-deacetylated chitin nanofibers (S-DACNFs).
25542790	1	9	theme	adhesive	179:186	arg1	properties					188:197	their adhesive properties	173:197	their adhesive properties	173:197	Novel biological adhesives made from chitin derivatives were prepared and evaluated for their adhesive properties and biocompatibility.
25542790	1	10	theme	Novel	85:89	arg1	adhesives					102:110	Novel biological adhesives	85:110	Novel biological adhesives made from chitin derivatives	85:139	Novel biological adhesives made from chitin derivatives were prepared and evaluated for their adhesive properties and biocompatibility.
25542790	1	11	theme	biological	91:100	arg1	adhesives					102:110	Novel biological adhesives	85:110	Novel biological adhesives made from chitin derivatives	85:139	Novel biological adhesives made from chitin derivatives were prepared and evaluated for their adhesive properties and biocompatibility.
25542790	2	12	theme	solution	420:427	arg1	addition					379:386	the addition	375:386	the addition of an aqueous hydrogen peroxide solution	375:427	Chitin derivatives with acrylic groups, such as 2-hydroxy-3-methacryloyloxypropylated carboxymethyl chitin (HMA-CM-chitin), were synthesized and cured by the addition of an aqueous hydrogen peroxide solution as a radical initiator.
25542790	4	13	contain	have	651:654	arg2	strength					678:685	almost equal adhesive strength	656:685	almost equal adhesive strength	656:685	HMA-CM-chitin/CNFs or HMA-CM-chitin/S-DACNFs have almost equal adhesive strength compared to that of a commercial cyanoacrylate adhesive.
25542790	4	13	contain	have	651:654	arg1	HMA-CM-chitin/CNFs					606:623	HMA-CM-chitin/CNFs	606:623	HMA-CM-chitin/CNFs	606:623	HMA-CM-chitin/CNFs or HMA-CM-chitin/S-DACNFs have almost equal adhesive strength compared to that of a commercial cyanoacrylate adhesive.
25542790	4	13	contain	have	651:654	arg1	HMA-CM-chitin/S-DACNFs					628:649	HMA-CM-chitin/S-DACNFs	628:649	HMA-CM-chitin/S-DACNFs	628:649	HMA-CM-chitin/CNFs or HMA-CM-chitin/S-DACNFs have almost equal adhesive strength compared to that of a commercial cyanoacrylate adhesive.
25542790	6	14	theme	biological	975:984	arg1	adhesives					986:994	new biological adhesives	971:994	new biological adhesives	971:994	These findings indicate that the composites prepared in this study are promising materials as new biological adhesives.
25542790	0	15	theme	chitin	43:48	arg1	derivatives					50:60	carboxymethyl chitin derivatives	29:60	carboxymethyl chitin derivatives	29:60	Biological adhesive based on carboxymethyl chitin derivatives and chitin nanofibers.
25542790	5	16	theme	inflammatory	786:797	arg1	cells					799:803	inflammatory cells	786:803	inflammatory cells	786:803	Moreover, quick adhesion and induction of inflammatory cells migration were observed in HMA-CM-chitin/CNF and HMA-CM-chitin/S-DACNF.
25542790	6	17	theme	new	971:973	arg1	adhesives					986:994	new biological adhesives	971:994	new biological adhesives	971:994	These findings indicate that the composites prepared in this study are promising materials as new biological adhesives.
25542790	5	18	theme	cells	799:803	arg1	adhesion					760:767	quick adhesion	754:767	quick adhesion	754:767	Moreover, quick adhesion and induction of inflammatory cells migration were observed in HMA-CM-chitin/CNF and HMA-CM-chitin/S-DACNF.
25542790	5	18	theme	cells	799:803	arg1	induction					773:781	induction	773:781	induction of inflammatory cells	773:803	Moreover, quick adhesion and induction of inflammatory cells migration were observed in HMA-CM-chitin/CNF and HMA-CM-chitin/S-DACNF.
25542790	4	19	theme	commercial	709:718	arg1	cyanoacrylate					720:732	a commercial cyanoacrylate adhesive	707:741	a commercial cyanoacrylate adhesive	707:741	HMA-CM-chitin/CNFs or HMA-CM-chitin/S-DACNFs have almost equal adhesive strength compared to that of a commercial cyanoacrylate adhesive.
25542790	3	20	theme	HMA-CM-chitin	478:490	arg1	strength					466:473	The adhesive strength	453:473	The adhesive strength of HMA-CM-chitin	453:490	The adhesive strength of HMA-CM-chitin increased when it was blended with chitin nanofibers (CNFs) or surface-deacetylated chitin nanofibers (S-DACNFs).
25542790	3	21	theme	adhesive	457:464	arg1	strength					466:473	The adhesive strength	453:473	The adhesive strength of HMA-CM-chitin	453:490	The adhesive strength of HMA-CM-chitin increased when it was blended with chitin nanofibers (CNFs) or surface-deacetylated chitin nanofibers (S-DACNFs).
25542790	1	22	theme	chitin	122:127	arg1	derivatives					129:139	chitin derivatives	122:139	chitin derivatives	122:139	Novel biological adhesives made from chitin derivatives were prepared and evaluated for their adhesive properties and biocompatibility.
25542790	2	23	theme	hydrogen	402:409	arg1	peroxide					411:418	an aqueous hydrogen peroxide	391:418	an aqueous hydrogen peroxide solution	391:427	Chitin derivatives with acrylic groups, such as 2-hydroxy-3-methacryloyloxypropylated carboxymethyl chitin (HMA-CM-chitin), were synthesized and cured by the addition of an aqueous hydrogen peroxide solution as a radical initiator.
25542790	5	24	theme	quick	754:758	arg1	adhesion					760:767	quick adhesion	754:767	quick adhesion	754:767	Moreover, quick adhesion and induction of inflammatory cells migration were observed in HMA-CM-chitin/CNF and HMA-CM-chitin/S-DACNF.
25542790	6	25	theme	promising	948:956	arg1	composites					910:919	the composites	906:919	the composites prepared in this study	906:942	These findings indicate that the composites prepared in this study are promising materials as new biological adhesives.
25542790	6	25	theme	promising	948:956	arg1	materials					958:966	promising materials	948:966	promising materials	948:966	These findings indicate that the composites prepared in this study are promising materials as new biological adhesives.
25542790	2	26	theme	acrylic	245:251	arg1	groups					253:258	acrylic groups	245:258	acrylic groups	245:258	Chitin derivatives with acrylic groups, such as 2-hydroxy-3-methacryloyloxypropylated carboxymethyl chitin (HMA-CM-chitin), were synthesized and cured by the addition of an aqueous hydrogen peroxide solution as a radical initiator.
25542790	2	27	theme	Chitin	221:226	arg1	derivatives					228:238	Chitin derivatives	221:238	Chitin derivatives	221:238	Chitin derivatives with acrylic groups, such as 2-hydroxy-3-methacryloyloxypropylated carboxymethyl chitin (HMA-CM-chitin), were synthesized and cured by the addition of an aqueous hydrogen peroxide solution as a radical initiator.
25542790	2	27	theme	Chitin	221:226	arg1	initiator					442:450	a radical initiator	432:450	a radical initiator	432:450	Chitin derivatives with acrylic groups, such as 2-hydroxy-3-methacryloyloxypropylated carboxymethyl chitin (HMA-CM-chitin), were synthesized and cured by the addition of an aqueous hydrogen peroxide solution as a radical initiator.
25542790	2	27	theme	Chitin	221:226	arg1	chitin					321:326	2-hydroxy-3-methacryloyloxypropylated carboxymethyl chitin	269:326	2-hydroxy-3-methacryloyloxypropylated carboxymethyl chitin (HMA-CM-chitin)	269:342	Chitin derivatives with acrylic groups, such as 2-hydroxy-3-methacryloyloxypropylated carboxymethyl chitin (HMA-CM-chitin), were synthesized and cured by the addition of an aqueous hydrogen peroxide solution as a radical initiator.
25542790	2	28	theme	carboxymethyl	307:319	arg1	HMA-CM-chitin					329:341	HMA-CM-chitin	329:341	HMA-CM-chitin	329:341	Chitin derivatives with acrylic groups, such as 2-hydroxy-3-methacryloyloxypropylated carboxymethyl chitin (HMA-CM-chitin), were synthesized and cured by the addition of an aqueous hydrogen peroxide solution as a radical initiator.
25542790	2	28	theme	carboxymethyl	307:319	arg1	chitin					321:326	2-hydroxy-3-methacryloyloxypropylated carboxymethyl chitin	269:326	2-hydroxy-3-methacryloyloxypropylated carboxymethyl chitin (HMA-CM-chitin)	269:342	Chitin derivatives with acrylic groups, such as 2-hydroxy-3-methacryloyloxypropylated carboxymethyl chitin (HMA-CM-chitin), were synthesized and cured by the addition of an aqueous hydrogen peroxide solution as a radical initiator.
25542790	3	29	theme	chitin	527:532	arg1	nanofibers					534:543	chitin nanofibers	527:543	chitin nanofibers (CNFs)	527:550	The adhesive strength of HMA-CM-chitin increased when it was blended with chitin nanofibers (CNFs) or surface-deacetylated chitin nanofibers (S-DACNFs).
25542790	3	29	theme	chitin	527:532	arg1	CNFs					546:549	CNFs	546:549	CNFs	546:549	The adhesive strength of HMA-CM-chitin increased when it was blended with chitin nanofibers (CNFs) or surface-deacetylated chitin nanofibers (S-DACNFs).
25542790	4	30	theme	adhesive	669:676	arg1	strength					678:685	almost equal adhesive strength	656:685	almost equal adhesive strength	656:685	HMA-CM-chitin/CNFs or HMA-CM-chitin/S-DACNFs have almost equal adhesive strength compared to that of a commercial cyanoacrylate adhesive.
25542790	2	31	theme	2-hydroxy-3-methacryloyloxypropylated	269:305	arg1	HMA-CM-chitin					329:341	HMA-CM-chitin	329:341	HMA-CM-chitin	329:341	Chitin derivatives with acrylic groups, such as 2-hydroxy-3-methacryloyloxypropylated carboxymethyl chitin (HMA-CM-chitin), were synthesized and cured by the addition of an aqueous hydrogen peroxide solution as a radical initiator.
25542790	2	31	theme	2-hydroxy-3-methacryloyloxypropylated	269:305	arg1	chitin					321:326	2-hydroxy-3-methacryloyloxypropylated carboxymethyl chitin	269:326	2-hydroxy-3-methacryloyloxypropylated carboxymethyl chitin (HMA-CM-chitin)	269:342	Chitin derivatives with acrylic groups, such as 2-hydroxy-3-methacryloyloxypropylated carboxymethyl chitin (HMA-CM-chitin), were synthesized and cured by the addition of an aqueous hydrogen peroxide solution as a radical initiator.
25542790	2	32	theme	peroxide	411:418	arg1	solution					420:427	an aqueous hydrogen peroxide solution	391:427	an aqueous hydrogen peroxide solution	391:427	Chitin derivatives with acrylic groups, such as 2-hydroxy-3-methacryloyloxypropylated carboxymethyl chitin (HMA-CM-chitin), were synthesized and cured by the addition of an aqueous hydrogen peroxide solution as a radical initiator.
25542790	0	33	theme	chitin	66:71	arg1	nanofibers					73:82	chitin nanofibers	66:82	chitin nanofibers	66:82	Biological adhesive based on carboxymethyl chitin derivatives and chitin nanofibers.
25542790	3	34	theme	surface-deacetylated	555:574	arg1	S-DACNFs					595:602	S-DACNFs	595:602	S-DACNFs	595:602	The adhesive strength of HMA-CM-chitin increased when it was blended with chitin nanofibers (CNFs) or surface-deacetylated chitin nanofibers (S-DACNFs).
25542790	3	34	theme	surface-deacetylated	555:574	arg1	nanofibers					583:592	surface-deacetylated chitin nanofibers	555:592	surface-deacetylated chitin nanofibers (S-DACNFs)	555:603	The adhesive strength of HMA-CM-chitin increased when it was blended with chitin nanofibers (CNFs) or surface-deacetylated chitin nanofibers (S-DACNFs).
24727117	5	0	theme	significant	818:828	arg1	degree					830:835	a significant degree	816:835	a significant degree of corrosion protection of stainless steel	816:878	Electrochemical testing demonstrated that a significant degree of corrosion protection of stainless steel could be achieved when CNs-containing coatings were present.
24727117	6	1	theme	free-standing	992:1004	arg1	membranes					1019:1027	free-standing CNs/alginate membranes	992:1027	free-standing CNs/alginate membranes	992:1027	Additionally, the one-step EPD-based processing of free-standing CNs/alginate membranes was demonstrated confirming the versatility of EPD to fabricate free-standing membrane structures compared to a layer-by-layer deposition technique.
24727117	4	2	theme	coatings	736:743	arg1	roughness					690:698	nanotopographic surface roughness	666:698	nanotopographic surface roughness	666:698	The nanotopographic surface roughness and hydrophilicity of the deposited coatings were measured and discussed.
24727117	4	2	theme	coatings	736:743	arg1	hydrophilicity					704:717	hydrophilicity	704:717	hydrophilicity	704:717	The nanotopographic surface roughness and hydrophilicity of the deposited coatings were measured and discussed.
24727117	1	3	theme	coatings	250:257	arg1	EPD					180:182	EPD	180:182	EPD	180:182	This study presents the electrophoretic deposition (EPD) of cellulose nanocrystals (CNs) and CNs-based alginate composite coatings for biomedical applications.
24727117	1	3	theme	coatings	250:257	arg1	deposition					168:177	the electrophoretic deposition	148:177	the electrophoretic deposition (EPD) of cellulose nanocrystals (CNs) and CNs-based alginate composite coatings for biomedical applications	148:285	This study presents the electrophoretic deposition (EPD) of cellulose nanocrystals (CNs) and CNs-based alginate composite coatings for biomedical applications.
24727117	2	4	theme	composites	364:373	arg1	co-deposition					334:346	co-deposition	334:346	co-deposition	334:346	The mechanism of anodic deposition of CNs and co-deposition of CNs/alginate composites was analyzed based on the results of zeta-potential, Fourier transform infrared spectroscopy and scanning electron microscopy (SEM) analyses.
24727117	2	4	theme	composites	364:373	arg1	CNs					326:328	CNs	326:328	CNs	326:328	The mechanism of anodic deposition of CNs and co-deposition of CNs/alginate composites was analyzed based on the results of zeta-potential, Fourier transform infrared spectroscopy and scanning electron microscopy (SEM) analyses.
24727117	6	5	theme	free-standing	1093:1105	arg1	structures					1116:1125	free-standing membrane structures	1093:1125	free-standing membrane structures	1093:1125	Additionally, the one-step EPD-based processing of free-standing CNs/alginate membranes was demonstrated confirming the versatility of EPD to fabricate free-standing membrane structures compared to a layer-by-layer deposition technique.
24727117	0	6	theme	free	103:106	arg1	membranes					117:125	free standing membranes	103:125	free standing membranes	103:125	Electrophoretic deposition of cellulose nanocrystals (CNs) and CNs/alginate nanocomposite coatings and free standing membranes.
24727117	6	7	theme	deposition	1156:1165	arg1	technique					1167:1175	a layer-by-layer deposition technique	1139:1175	a layer-by-layer deposition technique	1139:1175	Additionally, the one-step EPD-based processing of free-standing CNs/alginate membranes was demonstrated confirming the versatility of EPD to fabricate free-standing membrane structures compared to a layer-by-layer deposition technique.
24727117	1	8	theme	biomedical	263:272	arg1	applications					274:285	biomedical applications	263:285	biomedical applications	263:285	This study presents the electrophoretic deposition (EPD) of cellulose nanocrystals (CNs) and CNs-based alginate composite coatings for biomedical applications.
24727117	3	9	theme	CNs	589:591	arg1	orientation					574:584	the orientation	570:584	the orientation of CNs	570:591	The capability of the EPD technique for manipulating the orientation of CNs and for the preparation of multilayer CNs coatings was demonstrated.
24727117	6	10	theme	EPD-based	968:976	arg1	processing					978:987	the one-step EPD-based processing	955:987	the one-step EPD-based processing of free-standing CNs/alginate membranes	955:1027	Additionally, the one-step EPD-based processing of free-standing CNs/alginate membranes was demonstrated confirming the versatility of EPD to fabricate free-standing membrane structures compared to a layer-by-layer deposition technique.
24727117	7	11	theme	potential	1242:1250	arg1	coatings					1213:1220	CNs and CNs/alginate nanocomposite coatings	1178:1220	CNs and CNs/alginate nanocomposite coatings produced by EPD	1178:1236	CNs and CNs/alginate nanocomposite coatings produced by EPD are potential candidates for biomedical, cell technology and drug delivery applications.
24727117	7	11	theme	potential	1242:1250	arg1	candidates					1252:1261	potential candidates	1242:1261	potential candidates for biomedical, cell technology and drug delivery applications	1242:1324	CNs and CNs/alginate nanocomposite coatings produced by EPD are potential candidates for biomedical, cell technology and drug delivery applications.
24727117	4	12	theme	deposited	726:734	arg1	coatings					736:743	the deposited coatings	722:743	the deposited coatings	722:743	The nanotopographic surface roughness and hydrophilicity of the deposited coatings were measured and discussed.
24727117	6	13	theme	one-step	959:966	arg1	processing					978:987	the one-step EPD-based processing	955:987	the one-step EPD-based processing of free-standing CNs/alginate membranes	955:1027	Additionally, the one-step EPD-based processing of free-standing CNs/alginate membranes was demonstrated confirming the versatility of EPD to fabricate free-standing membrane structures compared to a layer-by-layer deposition technique.
24727117	2	14	dep	spectroscopy	455:466	arg1	analyses					507:514	analyses	507:514	analyses	507:514	The mechanism of anodic deposition of CNs and co-deposition of CNs/alginate composites was analyzed based on the results of zeta-potential, Fourier transform infrared spectroscopy and scanning electron microscopy (SEM) analyses.
24727117	5	15	theme	stainless	864:872	arg1	steel					874:878	stainless steel	864:878	stainless steel	864:878	Electrochemical testing demonstrated that a significant degree of corrosion protection of stainless steel could be achieved when CNs-containing coatings were present.
24727117	3	16	theme	technique	543:551	arg1	capability					521:530	The capability	517:530	The capability of the EPD technique for manipulating the orientation of CNs and for the preparation of multilayer CNs coatings	517:642	The capability of the EPD technique for manipulating the orientation of CNs and for the preparation of multilayer CNs coatings was demonstrated.
24727117	0	17	theme	standing	108:115	arg1	membranes					117:125	free standing membranes	103:125	free standing membranes	103:125	Electrophoretic deposition of cellulose nanocrystals (CNs) and CNs/alginate nanocomposite coatings and free standing membranes.
24727117	6	18	theme	membrane	1107:1114	arg1	structures					1116:1125	free-standing membrane structures	1093:1125	free-standing membrane structures	1093:1125	Additionally, the one-step EPD-based processing of free-standing CNs/alginate membranes was demonstrated confirming the versatility of EPD to fabricate free-standing membrane structures compared to a layer-by-layer deposition technique.
24727117	5	19	theme	steel	874:878	arg1	protection					850:859	corrosion protection	840:859	corrosion protection of stainless steel	840:878	Electrochemical testing demonstrated that a significant degree of corrosion protection of stainless steel could be achieved when CNs-containing coatings were present.
24727117	4	20	theme	surface	682:688	arg1	roughness					690:698	nanotopographic surface roughness	666:698	nanotopographic surface roughness	666:698	The nanotopographic surface roughness and hydrophilicity of the deposited coatings were measured and discussed.
24727117	2	21	theme	scanning	472:479	arg1	microscopy					490:499	scanning electron microscopy	472:499	scanning electron microscopy (SEM)	472:505	The mechanism of anodic deposition of CNs and co-deposition of CNs/alginate composites was analyzed based on the results of zeta-potential, Fourier transform infrared spectroscopy and scanning electron microscopy (SEM) analyses.
24727117	2	21	theme	scanning	472:479	arg1	SEM					502:504	SEM	502:504	SEM	502:504	The mechanism of anodic deposition of CNs and co-deposition of CNs/alginate composites was analyzed based on the results of zeta-potential, Fourier transform infrared spectroscopy and scanning electron microscopy (SEM) analyses.
24727117	2	22	theme	CNs	326:328	arg1	deposition					312:321	anodic deposition	305:321	anodic deposition of CNs and co-deposition of CNs/alginate composites	305:373	The mechanism of anodic deposition of CNs and co-deposition of CNs/alginate composites was analyzed based on the results of zeta-potential, Fourier transform infrared spectroscopy and scanning electron microscopy (SEM) analyses.
24727117	0	23	theme	Electrophoretic	0:14	arg1	deposition					16:25	Electrophoretic deposition	0:25	Electrophoretic deposition of cellulose nanocrystals (CNs)	0:57	Electrophoretic deposition of cellulose nanocrystals (CNs) and CNs/alginate nanocomposite coatings and free standing membranes.
24727117	6	24	theme	EPD	1076:1078	arg1	versatility					1061:1071	the versatility	1057:1071	the versatility of EPD to fabricate free-standing membrane structures compared to a layer-by-layer deposition technique	1057:1175	Additionally, the one-step EPD-based processing of free-standing CNs/alginate membranes was demonstrated confirming the versatility of EPD to fabricate free-standing membrane structures compared to a layer-by-layer deposition technique.
24727117	1	25	theme	cellulose	188:196	arg1	CNs					212:214	CNs	212:214	CNs	212:214	This study presents the electrophoretic deposition (EPD) of cellulose nanocrystals (CNs) and CNs-based alginate composite coatings for biomedical applications.
24727117	1	25	theme	cellulose	188:196	arg1	nanocrystals					198:209	cellulose nanocrystals	188:209	cellulose nanocrystals (CNs)	188:215	This study presents the electrophoretic deposition (EPD) of cellulose nanocrystals (CNs) and CNs-based alginate composite coatings for biomedical applications.
24727117	0	26	theme	cellulose	30:38	arg1	CNs					54:56	CNs	54:56	CNs	54:56	Electrophoretic deposition of cellulose nanocrystals (CNs) and CNs/alginate nanocomposite coatings and free standing membranes.
24727117	0	26	theme	cellulose	30:38	arg1	nanocrystals					40:51	cellulose nanocrystals	30:51	cellulose nanocrystals (CNs)	30:57	Electrophoretic deposition of cellulose nanocrystals (CNs) and CNs/alginate nanocomposite coatings and free standing membranes.
24727117	4	27	dep	roughness	690:698	arg1	The					662:664	The	662:664	The	662:664	The nanotopographic surface roughness and hydrophilicity of the deposited coatings were measured and discussed.
24727117	7	28	theme	CNs/alginate	1186:1197	arg1	coatings					1213:1220	CNs and CNs/alginate nanocomposite coatings	1178:1220	CNs and CNs/alginate nanocomposite coatings produced by EPD	1178:1236	CNs and CNs/alginate nanocomposite coatings produced by EPD are potential candidates for biomedical, cell technology and drug delivery applications.
24727117	7	28	theme	CNs/alginate	1186:1197	arg1	candidates					1252:1261	potential candidates	1242:1261	potential candidates for biomedical, cell technology and drug delivery applications	1242:1324	CNs and CNs/alginate nanocomposite coatings produced by EPD are potential candidates for biomedical, cell technology and drug delivery applications.
24727117	2	29	theme	deposition	312:321	arg1	mechanism					292:300	The mechanism	288:300	The mechanism of anodic deposition of CNs and co-deposition of CNs/alginate composites	288:373	The mechanism of anodic deposition of CNs and co-deposition of CNs/alginate composites was analyzed based on the results of zeta-potential, Fourier transform infrared spectroscopy and scanning electron microscopy (SEM) analyses.
24727117	1	30	theme	nanocrystals	198:209	arg1	EPD					180:182	EPD	180:182	EPD	180:182	This study presents the electrophoretic deposition (EPD) of cellulose nanocrystals (CNs) and CNs-based alginate composite coatings for biomedical applications.
24727117	1	30	theme	nanocrystals	198:209	arg1	deposition					168:177	the electrophoretic deposition	148:177	the electrophoretic deposition (EPD) of cellulose nanocrystals (CNs) and CNs-based alginate composite coatings for biomedical applications	148:285	This study presents the electrophoretic deposition (EPD) of cellulose nanocrystals (CNs) and CNs-based alginate composite coatings for biomedical applications.
24727117	7	31	theme	drug	1299:1302	arg1	delivery					1304:1311	drug delivery	1299:1311	drug delivery	1299:1311	CNs and CNs/alginate nanocomposite coatings produced by EPD are potential candidates for biomedical, cell technology and drug delivery applications.
24727117	7	31	theme	drug	1299:1302	arg1	applications					1313:1324	biomedical, cell technology and drug delivery applications	1267:1324	applications	1313:1324	CNs and CNs/alginate nanocomposite coatings produced by EPD are potential candidates for biomedical, cell technology and drug delivery applications.
24727117	7	32	theme	nanocomposite	1199:1211	arg1	coatings					1213:1220	CNs and CNs/alginate nanocomposite coatings	1178:1220	CNs and CNs/alginate nanocomposite coatings produced by EPD	1178:1236	CNs and CNs/alginate nanocomposite coatings produced by EPD are potential candidates for biomedical, cell technology and drug delivery applications.
24727117	7	32	theme	nanocomposite	1199:1211	arg1	candidates					1252:1261	potential candidates	1242:1261	potential candidates for biomedical, cell technology and drug delivery applications	1242:1324	CNs and CNs/alginate nanocomposite coatings produced by EPD are potential candidates for biomedical, cell technology and drug delivery applications.
24727117	2	33	theme	anodic	305:310	arg1	deposition					312:321	anodic deposition	305:321	anodic deposition of CNs and co-deposition of CNs/alginate composites	305:373	The mechanism of anodic deposition of CNs and co-deposition of CNs/alginate composites was analyzed based on the results of zeta-potential, Fourier transform infrared spectroscopy and scanning electron microscopy (SEM) analyses.
24727117	7	34	theme	cell	1279:1282	arg1	technology					1284:1293	cell technology	1279:1293	cell technology	1279:1293	CNs and CNs/alginate nanocomposite coatings produced by EPD are potential candidates for biomedical, cell technology and drug delivery applications.
24727117	7	34	theme	cell	1279:1282	arg1	applications					1313:1324	biomedical, cell technology and drug delivery applications	1267:1324	applications	1313:1324	CNs and CNs/alginate nanocomposite coatings produced by EPD are potential candidates for biomedical, cell technology and drug delivery applications.
24727117	7	35	theme	CNs	1178:1180	arg1	coatings					1213:1220	CNs and CNs/alginate nanocomposite coatings	1178:1220	CNs and CNs/alginate nanocomposite coatings produced by EPD	1178:1236	CNs and CNs/alginate nanocomposite coatings produced by EPD are potential candidates for biomedical, cell technology and drug delivery applications.
24727117	7	35	theme	CNs	1178:1180	arg1	candidates					1252:1261	potential candidates	1242:1261	potential candidates for biomedical, cell technology and drug delivery applications	1242:1324	CNs and CNs/alginate nanocomposite coatings produced by EPD are potential candidates for biomedical, cell technology and drug delivery applications.
24727117	5	36	theme	CNs-containing	903:916	arg1	coatings					918:925	CNs-containing coatings	903:925	CNs-containing coatings	903:925	Electrochemical testing demonstrated that a significant degree of corrosion protection of stainless steel could be achieved when CNs-containing coatings were present.
24727117	4	37	theme	nanotopographic	666:680	arg1	roughness					690:698	nanotopographic surface roughness	666:698	nanotopographic surface roughness	666:698	The nanotopographic surface roughness and hydrophilicity of the deposited coatings were measured and discussed.
24727117	0	38	theme	nanocrystals	40:51	arg1	deposition					16:25	Electrophoretic deposition	0:25	Electrophoretic deposition of cellulose nanocrystals (CNs)	0:57	Electrophoretic deposition of cellulose nanocrystals (CNs) and CNs/alginate nanocomposite coatings and free standing membranes.
24727117	0	38	theme	nanocrystals	40:51	arg1	CNs/alginate					63:74	CNs/alginate	63:74	CNs/alginate	63:74	Electrophoretic deposition of cellulose nanocrystals (CNs) and CNs/alginate nanocomposite coatings and free standing membranes.
24727117	5	39	theme	corrosion	840:848	arg1	protection					850:859	corrosion protection	840:859	corrosion protection of stainless steel	840:878	Electrochemical testing demonstrated that a significant degree of corrosion protection of stainless steel could be achieved when CNs-containing coatings were present.
24727117	5	40	theme	Electrochemical	774:788	arg1	testing					790:796	Electrochemical testing	774:796	Electrochemical testing	774:796	Electrochemical testing demonstrated that a significant degree of corrosion protection of stainless steel could be achieved when CNs-containing coatings were present.
24727117	2	41	theme	zeta-potential	412:425	arg1	results					401:407	the results	397:407	the results of zeta-potential, Fourier transform infrared spectroscopy and scanning electron microscopy (SEM) analyses	397:514	The mechanism of anodic deposition of CNs and co-deposition of CNs/alginate composites was analyzed based on the results of zeta-potential, Fourier transform infrared spectroscopy and scanning electron microscopy (SEM) analyses.
24727117	5	42	theme	protection	850:859	arg1	degree					830:835	a significant degree	816:835	a significant degree of corrosion protection of stainless steel	816:878	Electrochemical testing demonstrated that a significant degree of corrosion protection of stainless steel could be achieved when CNs-containing coatings were present.
24727117	2	43	dep	transform	436:444	arg1	infrared					446:453	infrared	446:453	transform infrared spectroscopy and scanning electron microscopy (SEM) analyses	436:514	The mechanism of anodic deposition of CNs and co-deposition of CNs/alginate composites was analyzed based on the results of zeta-potential, Fourier transform infrared spectroscopy and scanning electron microscopy (SEM) analyses.
24727117	2	44	theme	CNs/alginate	351:362	arg1	composites					364:373	CNs/alginate composites	351:373	CNs/alginate composites	351:373	The mechanism of anodic deposition of CNs and co-deposition of CNs/alginate composites was analyzed based on the results of zeta-potential, Fourier transform infrared spectroscopy and scanning electron microscopy (SEM) analyses.
24727117	2	45	theme	electron	481:488	arg1	microscopy					490:499	scanning electron microscopy	472:499	scanning electron microscopy (SEM)	472:505	The mechanism of anodic deposition of CNs and co-deposition of CNs/alginate composites was analyzed based on the results of zeta-potential, Fourier transform infrared spectroscopy and scanning electron microscopy (SEM) analyses.
24727117	2	45	theme	electron	481:488	arg1	SEM					502:504	SEM	502:504	SEM	502:504	The mechanism of anodic deposition of CNs and co-deposition of CNs/alginate composites was analyzed based on the results of zeta-potential, Fourier transform infrared spectroscopy and scanning electron microscopy (SEM) analyses.
24727117	2	46	dep	Fourier	428:434	arg1	transform					436:444	transform	436:444	transform infrared spectroscopy and scanning electron microscopy (SEM) analyses	436:514	The mechanism of anodic deposition of CNs and co-deposition of CNs/alginate composites was analyzed based on the results of zeta-potential, Fourier transform infrared spectroscopy and scanning electron microscopy (SEM) analyses.
24727117	6	47	theme	membranes	1019:1027	arg1	processing					978:987	the one-step EPD-based processing	955:987	the one-step EPD-based processing of free-standing CNs/alginate membranes	955:1027	Additionally, the one-step EPD-based processing of free-standing CNs/alginate membranes was demonstrated confirming the versatility of EPD to fabricate free-standing membrane structures compared to a layer-by-layer deposition technique.
24727117	7	48	dep	applications	1313:1324	arg1	technology					1284:1293	cell technology	1279:1293	cell technology	1279:1293	CNs and CNs/alginate nanocomposite coatings produced by EPD are potential candidates for biomedical, cell technology and drug delivery applications.
24727117	7	48	dep	applications	1313:1324	arg1	delivery					1304:1311	drug delivery	1299:1311	drug delivery	1299:1311	CNs and CNs/alginate nanocomposite coatings produced by EPD are potential candidates for biomedical, cell technology and drug delivery applications.
24727117	7	48	dep	applications	1313:1324	arg1	applications					1313:1324	biomedical, cell technology and drug delivery applications	1267:1324	applications	1313:1324	CNs and CNs/alginate nanocomposite coatings produced by EPD are potential candidates for biomedical, cell technology and drug delivery applications.
24727117	3	49	theme	coatings	635:642	arg1	preparation					605:615	the preparation	601:615	the preparation of multilayer CNs coatings	601:642	The capability of the EPD technique for manipulating the orientation of CNs and for the preparation of multilayer CNs coatings was demonstrated.
24727117	2	50	theme	co-deposition	334:346	arg1	deposition					312:321	anodic deposition	305:321	anodic deposition of CNs and co-deposition of CNs/alginate composites	305:373	The mechanism of anodic deposition of CNs and co-deposition of CNs/alginate composites was analyzed based on the results of zeta-potential, Fourier transform infrared spectroscopy and scanning electron microscopy (SEM) analyses.
24727117	3	51	theme	EPD	539:541	arg1	technique					543:551	the EPD technique	535:551	the EPD technique	535:551	The capability of the EPD technique for manipulating the orientation of CNs and for the preparation of multilayer CNs coatings was demonstrated.
24727117	1	52	theme	CNs-based	221:229	arg1	coatings					250:257	CNs-based alginate composite coatings	221:257	CNs-based alginate composite coatings	221:257	This study presents the electrophoretic deposition (EPD) of cellulose nanocrystals (CNs) and CNs-based alginate composite coatings for biomedical applications.
24727117	6	53	theme	layer-by-layer	1141:1154	arg1	technique					1167:1175	a layer-by-layer deposition technique	1139:1175	a layer-by-layer deposition technique	1139:1175	Additionally, the one-step EPD-based processing of free-standing CNs/alginate membranes was demonstrated confirming the versatility of EPD to fabricate free-standing membrane structures compared to a layer-by-layer deposition technique.
24727117	3	54	theme	multilayer	620:629	arg1	coatings					635:642	multilayer CNs coatings	620:642	multilayer CNs coatings	620:642	The capability of the EPD technique for manipulating the orientation of CNs and for the preparation of multilayer CNs coatings was demonstrated.
24727117	1	55	theme	alginate	231:238	arg1	coatings					250:257	CNs-based alginate composite coatings	221:257	CNs-based alginate composite coatings	221:257	This study presents the electrophoretic deposition (EPD) of cellulose nanocrystals (CNs) and CNs-based alginate composite coatings for biomedical applications.
24727117	7	56	theme	biomedical	1267:1276	arg1	technology					1284:1293	cell technology	1279:1293	cell technology	1279:1293	CNs and CNs/alginate nanocomposite coatings produced by EPD are potential candidates for biomedical, cell technology and drug delivery applications.
24727117	7	56	theme	biomedical	1267:1276	arg1	delivery					1304:1311	drug delivery	1299:1311	drug delivery	1299:1311	CNs and CNs/alginate nanocomposite coatings produced by EPD are potential candidates for biomedical, cell technology and drug delivery applications.
24727117	7	56	theme	biomedical	1267:1276	arg1	applications					1313:1324	biomedical, cell technology and drug delivery applications	1267:1324	applications	1313:1324	CNs and CNs/alginate nanocomposite coatings produced by EPD are potential candidates for biomedical, cell technology and drug delivery applications.
24727117	6	57	theme	CNs/alginate	1006:1017	arg1	membranes					1019:1027	free-standing CNs/alginate membranes	992:1027	free-standing CNs/alginate membranes	992:1027	Additionally, the one-step EPD-based processing of free-standing CNs/alginate membranes was demonstrated confirming the versatility of EPD to fabricate free-standing membrane structures compared to a layer-by-layer deposition technique.
24727117	3	58	theme	CNs	631:633	arg1	coatings					635:642	multilayer CNs coatings	620:642	multilayer CNs coatings	620:642	The capability of the EPD technique for manipulating the orientation of CNs and for the preparation of multilayer CNs coatings was demonstrated.
24727117	1	59	theme	electrophoretic	152:166	arg1	EPD					180:182	EPD	180:182	EPD	180:182	This study presents the electrophoretic deposition (EPD) of cellulose nanocrystals (CNs) and CNs-based alginate composite coatings for biomedical applications.
24727117	1	59	theme	electrophoretic	152:166	arg1	deposition					168:177	the electrophoretic deposition	148:177	the electrophoretic deposition (EPD) of cellulose nanocrystals (CNs) and CNs-based alginate composite coatings for biomedical applications	148:285	This study presents the electrophoretic deposition (EPD) of cellulose nanocrystals (CNs) and CNs-based alginate composite coatings for biomedical applications.
24727117	1	60	theme	composite	240:248	arg1	coatings					250:257	CNs-based alginate composite coatings	221:257	CNs-based alginate composite coatings	221:257	This study presents the electrophoretic deposition (EPD) of cellulose nanocrystals (CNs) and CNs-based alginate composite coatings for biomedical applications.
27616696	4	0	theme	bacteria	869:876	arg1	abundance					841:849	the relative abundance	828:849	the relative abundance of the beneficial bacteria such as Lactobacillales, Roseburia and Lachnospiraceae	828:931	At the genus level, supplementation of GLPS3 increased the relative abundance of the beneficial bacteria such as Lactobacillales, Roseburia and Lachnospiraceae.
27616696	1	1	from	S3	187:188	arg1	effects					130:136	the effects	126:136	the effects of polysaccharides from Ganoderma lucidum strain S3 (GLP S3) on chronic pancreatitis (CP) therapy and intestinal microbiota modulation in mice induced by diethyldithiocarbamate (DDC)	126:319	This study manifested the effects of polysaccharides from Ganoderma lucidum strain S3 (GLP S3) on chronic pancreatitis (CP) therapy and intestinal microbiota modulation in mice induced by diethyldithiocarbamate (DDC).
27616696	1	1	from	S3	187:188	arg1	polysaccharides					141:155	polysaccharides	141:155	polysaccharides from Ganoderma lucidum strain S3 (GLP S3)	141:197	This study manifested the effects of polysaccharides from Ganoderma lucidum strain S3 (GLP S3) on chronic pancreatitis (CP) therapy and intestinal microbiota modulation in mice induced by diethyldithiocarbamate (DDC).
27616696	1	2	theme	Ganoderma	162:170	arg1	S3					187:188	Ganoderma lucidum strain S3	162:188	Ganoderma lucidum strain S3 (GLP S3)	162:197	This study manifested the effects of polysaccharides from Ganoderma lucidum strain S3 (GLP S3) on chronic pancreatitis (CP) therapy and intestinal microbiota modulation in mice induced by diethyldithiocarbamate (DDC).
27616696	1	2	theme	Ganoderma	162:170	arg1	S3					195:196	GLP S3	191:196	GLP S3	191:196	This study manifested the effects of polysaccharides from Ganoderma lucidum strain S3 (GLP S3) on chronic pancreatitis (CP) therapy and intestinal microbiota modulation in mice induced by diethyldithiocarbamate (DDC).
27616696	3	3	theme	Bacteroidetes	717:729	arg1	abundance					697:705	the relative abundance	684:705	the relative abundance of phylum Bacteroidetes	684:729	Furthermore, high throughput sequencing analysis revealed that GLPS3 altered the composition and diversity of intestinal microbiota, especially, decreased the relative abundance of phylum Bacteroidetes and increased that of phylum Firmictutes.
27616696	2	4	theme	TNF-α	459:463	arg1	level					465:469	TNF-α level	459:469	TNF-α level	459:469	The GLPS3 was prepared from cultured mycelium and markedly alleviated the pancreatitis in mice through decreasing lipase, AMS, IFN-γ and TNF-α level as well as increasing SOD and total antioxidant activity.
27616696	4	5	theme	beneficial	858:867	arg1	Roseburia					903:911	Roseburia	903:911	Roseburia	903:911	At the genus level, supplementation of GLPS3 increased the relative abundance of the beneficial bacteria such as Lactobacillales, Roseburia and Lachnospiraceae.
27616696	4	5	theme	beneficial	858:867	arg1	bacteria					869:876	the beneficial bacteria	854:876	the beneficial bacteria such as Lactobacillales, Roseburia and Lachnospiraceae	854:931	At the genus level, supplementation of GLPS3 increased the relative abundance of the beneficial bacteria such as Lactobacillales, Roseburia and Lachnospiraceae.
27616696	4	5	theme	beneficial	858:867	arg1	Lactobacillales					886:900	Lactobacillales	886:900	Lactobacillales	886:900	At the genus level, supplementation of GLPS3 increased the relative abundance of the beneficial bacteria such as Lactobacillales, Roseburia and Lachnospiraceae.
27616696	4	5	theme	beneficial	858:867	arg1	Lachnospiraceae					917:931	Lachnospiraceae	917:931	Lachnospiraceae	917:931	At the genus level, supplementation of GLPS3 increased the relative abundance of the beneficial bacteria such as Lactobacillales, Roseburia and Lachnospiraceae.
27616696	1	6	theme	lucidum	172:178	arg1	S3					187:188	Ganoderma lucidum strain S3	162:188	Ganoderma lucidum strain S3 (GLP S3)	162:197	This study manifested the effects of polysaccharides from Ganoderma lucidum strain S3 (GLP S3) on chronic pancreatitis (CP) therapy and intestinal microbiota modulation in mice induced by diethyldithiocarbamate (DDC).
27616696	1	6	theme	lucidum	172:178	arg1	S3					195:196	GLP S3	191:196	GLP S3	191:196	This study manifested the effects of polysaccharides from Ganoderma lucidum strain S3 (GLP S3) on chronic pancreatitis (CP) therapy and intestinal microbiota modulation in mice induced by diethyldithiocarbamate (DDC).
27616696	5	7	from	mechanism	980:988	arg1	pancreatitis					1010:1021	chronic pancreatitis	1002:1021	chronic pancreatitis	1002:1021	These results disclosed the potential therapy mechanism of GLPS3 on chronic pancreatitis might be intestinal microbiota dependent.
27616696	0	8	from	Effects	0:6	arg1	microbiota					84:93	intestinal microbiota	73:93	intestinal microbiota	73:93	Effects of Ganoderma lucidum polysaccharides on chronic pancreatitis and intestinal microbiota in mice.
27616696	0	8	from	Effects	0:6	arg1	pancreatitis					56:67	chronic pancreatitis	48:67	chronic pancreatitis	48:67	Effects of Ganoderma lucidum polysaccharides on chronic pancreatitis and intestinal microbiota in mice.
27616696	1	9	theme	intestinal	240:249	arg1	modulation					262:271	intestinal microbiota modulation	240:271	intestinal microbiota modulation in mice induced by diethyldithiocarbamate (DDC)	240:319	This study manifested the effects of polysaccharides from Ganoderma lucidum strain S3 (GLP S3) on chronic pancreatitis (CP) therapy and intestinal microbiota modulation in mice induced by diethyldithiocarbamate (DDC).
27616696	0	10	from	pancreatitis	56:67	arg1	mice					98:101	mice	98:101	mice	98:101	Effects of Ganoderma lucidum polysaccharides on chronic pancreatitis and intestinal microbiota in mice.
27616696	1	11	theme	microbiota	251:260	arg1	modulation					262:271	intestinal microbiota modulation	240:271	intestinal microbiota modulation in mice induced by diethyldithiocarbamate (DDC)	240:319	This study manifested the effects of polysaccharides from Ganoderma lucidum strain S3 (GLP S3) on chronic pancreatitis (CP) therapy and intestinal microbiota modulation in mice induced by diethyldithiocarbamate (DDC).
27616696	5	12	theme	GLPS3	993:997	arg1	dependent					1054:1062	dependent	1054:1062	dependent	1054:1062	These results disclosed the potential therapy mechanism of GLPS3 on chronic pancreatitis might be intestinal microbiota dependent.
27616696	5	12	theme	GLPS3	993:997	arg1	mechanism					980:988	the potential therapy mechanism	958:988	the potential therapy mechanism of GLPS3 on chronic pancreatitis	958:1021	These results disclosed the potential therapy mechanism of GLPS3 on chronic pancreatitis might be intestinal microbiota dependent.
27616696	1	13	theme	strain	180:185	arg1	S3					187:188	Ganoderma lucidum strain S3	162:188	Ganoderma lucidum strain S3 (GLP S3)	162:197	This study manifested the effects of polysaccharides from Ganoderma lucidum strain S3 (GLP S3) on chronic pancreatitis (CP) therapy and intestinal microbiota modulation in mice induced by diethyldithiocarbamate (DDC).
27616696	1	13	theme	strain	180:185	arg1	S3					195:196	GLP S3	191:196	GLP S3	191:196	This study manifested the effects of polysaccharides from Ganoderma lucidum strain S3 (GLP S3) on chronic pancreatitis (CP) therapy and intestinal microbiota modulation in mice induced by diethyldithiocarbamate (DDC).
27616696	4	14	theme	relative	832:839	arg1	abundance					841:849	the relative abundance	828:849	the relative abundance of the beneficial bacteria such as Lactobacillales, Roseburia and Lachnospiraceae	828:931	At the genus level, supplementation of GLPS3 increased the relative abundance of the beneficial bacteria such as Lactobacillales, Roseburia and Lachnospiraceae.
27616696	1	15	from	effects	130:136	arg1	modulation					262:271	intestinal microbiota modulation	240:271	intestinal microbiota modulation in mice induced by diethyldithiocarbamate (DDC)	240:319	This study manifested the effects of polysaccharides from Ganoderma lucidum strain S3 (GLP S3) on chronic pancreatitis (CP) therapy and intestinal microbiota modulation in mice induced by diethyldithiocarbamate (DDC).
27616696	1	15	from	effects	130:136	arg1	S3					195:196	GLP S3	191:196	GLP S3	191:196	This study manifested the effects of polysaccharides from Ganoderma lucidum strain S3 (GLP S3) on chronic pancreatitis (CP) therapy and intestinal microbiota modulation in mice induced by diethyldithiocarbamate (DDC).
27616696	1	15	from	effects	130:136	arg1	S3					187:188	Ganoderma lucidum strain S3	162:188	Ganoderma lucidum strain S3 (GLP S3)	162:197	This study manifested the effects of polysaccharides from Ganoderma lucidum strain S3 (GLP S3) on chronic pancreatitis (CP) therapy and intestinal microbiota modulation in mice induced by diethyldithiocarbamate (DDC).
27616696	1	15	from	effects	130:136	arg1	therapy					228:234	chronic pancreatitis (CP) therapy	202:234	chronic pancreatitis (CP) therapy	202:234	This study manifested the effects of polysaccharides from Ganoderma lucidum strain S3 (GLP S3) on chronic pancreatitis (CP) therapy and intestinal microbiota modulation in mice induced by diethyldithiocarbamate (DDC).
27616696	3	16	theme	intestinal	639:648	arg1	microbiota					650:659	intestinal microbiota	639:659	intestinal microbiota	639:659	Furthermore, high throughput sequencing analysis revealed that GLPS3 altered the composition and diversity of intestinal microbiota, especially, decreased the relative abundance of phylum Bacteroidetes and increased that of phylum Firmictutes.
27616696	1	17	from	modulation	262:271	arg1	mice					276:279	mice	276:279	mice induced by diethyldithiocarbamate (DDC)	276:319	This study manifested the effects of polysaccharides from Ganoderma lucidum strain S3 (GLP S3) on chronic pancreatitis (CP) therapy and intestinal microbiota modulation in mice induced by diethyldithiocarbamate (DDC).
27616696	3	18	theme	high	542:545	arg1	analysis					569:576	high throughput sequencing analysis	542:576	high throughput sequencing analysis	542:576	Furthermore, high throughput sequencing analysis revealed that GLPS3 altered the composition and diversity of intestinal microbiota, especially, decreased the relative abundance of phylum Bacteroidetes and increased that of phylum Firmictutes.
27616696	2	19	theme	antioxidant	507:517	arg1	activity					519:526	total antioxidant activity	501:526	total antioxidant activity	501:526	The GLPS3 was prepared from cultured mycelium and markedly alleviated the pancreatitis in mice through decreasing lipase, AMS, IFN-γ and TNF-α level as well as increasing SOD and total antioxidant activity.
27616696	3	20	theme	throughput	547:556	arg1	analysis					569:576	high throughput sequencing analysis	542:576	high throughput sequencing analysis	542:576	Furthermore, high throughput sequencing analysis revealed that GLPS3 altered the composition and diversity of intestinal microbiota, especially, decreased the relative abundance of phylum Bacteroidetes and increased that of phylum Firmictutes.
27616696	1	21	theme	GLP	191:193	arg1	S3					187:188	Ganoderma lucidum strain S3	162:188	Ganoderma lucidum strain S3 (GLP S3)	162:197	This study manifested the effects of polysaccharides from Ganoderma lucidum strain S3 (GLP S3) on chronic pancreatitis (CP) therapy and intestinal microbiota modulation in mice induced by diethyldithiocarbamate (DDC).
27616696	1	21	theme	GLP	191:193	arg1	S3					195:196	GLP S3	191:196	GLP S3	191:196	This study manifested the effects of polysaccharides from Ganoderma lucidum strain S3 (GLP S3) on chronic pancreatitis (CP) therapy and intestinal microbiota modulation in mice induced by diethyldithiocarbamate (DDC).
27616696	0	22	theme	lucidum	21:27	arg1	polysaccharides					29:43	Ganoderma lucidum polysaccharides	11:43	Ganoderma lucidum polysaccharides	11:43	Effects of Ganoderma lucidum polysaccharides on chronic pancreatitis and intestinal microbiota in mice.
27616696	3	23	theme	phylum	753:758	arg1	Firmictutes					760:770	phylum Firmictutes	753:770	phylum Firmictutes	753:770	Furthermore, high throughput sequencing analysis revealed that GLPS3 altered the composition and diversity of intestinal microbiota, especially, decreased the relative abundance of phylum Bacteroidetes and increased that of phylum Firmictutes.
27616696	5	24	theme	potential	962:970	arg1	dependent					1054:1062	dependent	1054:1062	dependent	1054:1062	These results disclosed the potential therapy mechanism of GLPS3 on chronic pancreatitis might be intestinal microbiota dependent.
27616696	5	24	theme	potential	962:970	arg1	mechanism					980:988	the potential therapy mechanism	958:988	the potential therapy mechanism of GLPS3 on chronic pancreatitis	958:1021	These results disclosed the potential therapy mechanism of GLPS3 on chronic pancreatitis might be intestinal microbiota dependent.
27616696	5	25	theme	intestinal	1032:1041	arg1	microbiota					1043:1052	intestinal microbiota	1032:1052	intestinal microbiota	1032:1052	These results disclosed the potential therapy mechanism of GLPS3 on chronic pancreatitis might be intestinal microbiota dependent.
27616696	0	26	theme	Ganoderma	11:19	arg1	lucidum					21:27	Ganoderma lucidum	11:27	Ganoderma lucidum polysaccharides	11:43	Effects of Ganoderma lucidum polysaccharides on chronic pancreatitis and intestinal microbiota in mice.
27616696	5	27	theme	therapy	972:978	arg1	dependent					1054:1062	dependent	1054:1062	dependent	1054:1062	These results disclosed the potential therapy mechanism of GLPS3 on chronic pancreatitis might be intestinal microbiota dependent.
27616696	5	27	theme	therapy	972:978	arg1	mechanism					980:988	the potential therapy mechanism	958:988	the potential therapy mechanism of GLPS3 on chronic pancreatitis	958:1021	These results disclosed the potential therapy mechanism of GLPS3 on chronic pancreatitis might be intestinal microbiota dependent.
27616696	2	28	theme	cultured	350:357	arg1	mycelium					359:366	cultured mycelium	350:366	cultured mycelium	350:366	The GLPS3 was prepared from cultured mycelium and markedly alleviated the pancreatitis in mice through decreasing lipase, AMS, IFN-γ and TNF-α level as well as increasing SOD and total antioxidant activity.
27616696	1	29	from	therapy	228:234	arg1	mice					276:279	mice	276:279	mice induced by diethyldithiocarbamate (DDC)	276:319	This study manifested the effects of polysaccharides from Ganoderma lucidum strain S3 (GLP S3) on chronic pancreatitis (CP) therapy and intestinal microbiota modulation in mice induced by diethyldithiocarbamate (DDC).
27616696	0	30	from	microbiota	84:93	arg1	mice					98:101	mice	98:101	mice	98:101	Effects of Ganoderma lucidum polysaccharides on chronic pancreatitis and intestinal microbiota in mice.
27616696	4	31	theme	GLPS3	812:816	arg1	supplementation					793:807	supplementation	793:807	supplementation of GLPS3	793:816	At the genus level, supplementation of GLPS3 increased the relative abundance of the beneficial bacteria such as Lactobacillales, Roseburia and Lachnospiraceae.
27616696	0	32	theme	polysaccharides	29:43	arg1	Effects					0:6	Effects	0:6	Effects of Ganoderma lucidum polysaccharides on chronic pancreatitis and intestinal microbiota in mice	0:101	Effects of Ganoderma lucidum polysaccharides on chronic pancreatitis and intestinal microbiota in mice.
27616696	2	33	theme	total	501:505	arg1	activity					519:526	total antioxidant activity	501:526	total antioxidant activity	501:526	The GLPS3 was prepared from cultured mycelium and markedly alleviated the pancreatitis in mice through decreasing lipase, AMS, IFN-γ and TNF-α level as well as increasing SOD and total antioxidant activity.
27616696	1	34	theme	chronic	202:208	arg1	CP					224:225	CP	224:225	CP	224:225	This study manifested the effects of polysaccharides from Ganoderma lucidum strain S3 (GLP S3) on chronic pancreatitis (CP) therapy and intestinal microbiota modulation in mice induced by diethyldithiocarbamate (DDC).
27616696	1	34	theme	chronic	202:208	arg1	pancreatitis					210:221	chronic pancreatitis	202:221	chronic pancreatitis (CP) therapy	202:234	This study manifested the effects of polysaccharides from Ganoderma lucidum strain S3 (GLP S3) on chronic pancreatitis (CP) therapy and intestinal microbiota modulation in mice induced by diethyldithiocarbamate (DDC).
27616696	3	35	theme	sequencing	558:567	arg1	analysis					569:576	high throughput sequencing analysis	542:576	high throughput sequencing analysis	542:576	Furthermore, high throughput sequencing analysis revealed that GLPS3 altered the composition and diversity of intestinal microbiota, especially, decreased the relative abundance of phylum Bacteroidetes and increased that of phylum Firmictutes.
27616696	0	36	theme	chronic	48:54	arg1	pancreatitis					56:67	chronic pancreatitis	48:67	chronic pancreatitis	48:67	Effects of Ganoderma lucidum polysaccharides on chronic pancreatitis and intestinal microbiota in mice.
27616696	5	37	theme	chronic	1002:1008	arg1	pancreatitis					1010:1021	chronic pancreatitis	1002:1021	chronic pancreatitis	1002:1021	These results disclosed the potential therapy mechanism of GLPS3 on chronic pancreatitis might be intestinal microbiota dependent.
27616696	1	38	theme	pancreatitis	210:221	arg1	therapy					228:234	chronic pancreatitis (CP) therapy	202:234	chronic pancreatitis (CP) therapy	202:234	This study manifested the effects of polysaccharides from Ganoderma lucidum strain S3 (GLP S3) on chronic pancreatitis (CP) therapy and intestinal microbiota modulation in mice induced by diethyldithiocarbamate (DDC).
27616696	3	39	theme	microbiota	650:659	arg1	diversity					626:634	diversity	626:634	diversity of intestinal microbiota	626:659	Furthermore, high throughput sequencing analysis revealed that GLPS3 altered the composition and diversity of intestinal microbiota, especially, decreased the relative abundance of phylum Bacteroidetes and increased that of phylum Firmictutes.
27616696	3	39	theme	microbiota	650:659	arg1	composition					610:620	the composition	606:620	the composition	606:620	Furthermore, high throughput sequencing analysis revealed that GLPS3 altered the composition and diversity of intestinal microbiota, especially, decreased the relative abundance of phylum Bacteroidetes and increased that of phylum Firmictutes.
27616696	4	40	theme	genus	780:784	arg1	level					786:790	the genus level	776:790	the genus level	776:790	At the genus level, supplementation of GLPS3 increased the relative abundance of the beneficial bacteria such as Lactobacillales, Roseburia and Lachnospiraceae.
27616696	2	41	from	pancreatitis	396:407	arg1	mice					412:415	mice	412:415	mice	412:415	The GLPS3 was prepared from cultured mycelium and markedly alleviated the pancreatitis in mice through decreasing lipase, AMS, IFN-γ and TNF-α level as well as increasing SOD and total antioxidant activity.
27616696	0	42	theme	intestinal	73:82	arg1	microbiota					84:93	intestinal microbiota	73:93	intestinal microbiota	73:93	Effects of Ganoderma lucidum polysaccharides on chronic pancreatitis and intestinal microbiota in mice.
27616696	1	43	theme	polysaccharides	141:155	arg1	effects					130:136	the effects	126:136	the effects of polysaccharides from Ganoderma lucidum strain S3 (GLP S3) on chronic pancreatitis (CP) therapy and intestinal microbiota modulation in mice induced by diethyldithiocarbamate (DDC)	126:319	This study manifested the effects of polysaccharides from Ganoderma lucidum strain S3 (GLP S3) on chronic pancreatitis (CP) therapy and intestinal microbiota modulation in mice induced by diethyldithiocarbamate (DDC).
27616696	3	44	theme	relative	688:695	arg1	abundance					697:705	the relative abundance	684:705	the relative abundance of phylum Bacteroidetes	684:729	Furthermore, high throughput sequencing analysis revealed that GLPS3 altered the composition and diversity of intestinal microbiota, especially, decreased the relative abundance of phylum Bacteroidetes and increased that of phylum Firmictutes.
25627882	7	0	theme	G2	1098:1099	arg1	surfaces					1108:1115	the G2 and G3 surfaces	1094:1115	the G2 and G3 surfaces	1094:1115	Heterogeneously shaped particles were found on the G2 and G3 surfaces, and the cement matrices were randomly interspersed with long intermingled fibers.
25627882	9	1	dep	concentration	1367:1379	arg1	especially					1342:1351	especially	1342:1351	especially	1342:1351	The physically processed cellulosic fibers (especially at the higher concentration) increased the compressive and diametral tensile strengths of the GIC, and demonstrated acceptable elastic modulus and stiffness.
25627882	5	2	from	differences	771:781	arg1	modulus					794:800	elastic modulus	786:800	elastic modulus	786:800	Statistically significant differences in elastic modulus were also found between G2 and G1 and between G2 and G3, whereas the stiffness significantly differed between G1 and G2.
25627882	9	3	theme	compressive	1396:1406	arg1	strengths					1430:1438	the compressive and diametral tensile strengths	1392:1438	the compressive and diametral tensile strengths of the GIC	1392:1449	The physically processed cellulosic fibers (especially at the higher concentration) increased the compressive and diametral tensile strengths of the GIC, and demonstrated acceptable elastic modulus and stiffness.
25627882	0	4	theme	mechanical	72:81	arg1	properties					83:92	mechanical properties	72:92	mechanical properties	72:92	Addition of mechanically processed cellulosic fibers to ionomer cement: mechanical properties.
25627882	6	5	theme	dispersive	1010:1019	arg1	EDS					1041:1043	EDS	1041:1043	EDS	1041:1043	The materials were then characterized by scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS).
25627882	6	5	theme	dispersive	1010:1019	arg1	spectroscopy					1027:1038	energy dispersive X-ray spectroscopy	1003:1038	energy dispersive X-ray spectroscopy (EDS)	1003:1044	The materials were then characterized by scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS).
25627882	4	6	theme	tensile	683:689	arg1	strengths					691:699	The compressive and diametral tensile strengths	653:699	The compressive and diametral tensile strengths	653:699	The compressive and diametral tensile strengths were significantly higher in G3 than in G1.
25627882	4	6	theme	tensile	683:689	arg1	higher					720:725	higher	720:725	higher	720:725	The compressive and diametral tensile strengths were significantly higher in G3 than in G1.
25627882	2	7	dep	2	359:359	arg1	G2					362:363	G2	362:363	G2	362:363	Two concentrations of fibers were weighed and agglutinated into the GIC during manipulation, yielding Experimental Groups 2 (G2; 3.62 wt% of fibers) and 3 (G3; 7.24 wt% of fibers), which were compared against a control group containing no fibers (G1).
25627882	9	8	theme	processed	1313:1321	arg1	fibers					1334:1339	The physically processed cellulosic fibers	1298:1339	The physically processed cellulosic fibers (especially at the higher concentration)	1298:1380	The physically processed cellulosic fibers (especially at the higher concentration) increased the compressive and diametral tensile strengths of the GIC, and demonstrated acceptable elastic modulus and stiffness.
25627882	8	9	theme	elemental	1247:1255	arg1	compositions					1257:1268	the elemental compositions	1243:1268	the elemental compositions of the precursor materials	1243:1295	The EDS spectra of the composites revealed the elemental compositions of the precursor materials.
25627882	9	10	theme	GIC	1447:1449	arg1	strengths					1430:1438	the compressive and diametral tensile strengths	1392:1438	the compressive and diametral tensile strengths of the GIC	1392:1449	The physically processed cellulosic fibers (especially at the higher concentration) increased the compressive and diametral tensile strengths of the GIC, and demonstrated acceptable elastic modulus and stiffness.
25627882	2	11	dep	Groups	352:357	arg1	2					359:359	2	359:359	2	359:359	Two concentrations of fibers were weighed and agglutinated into the GIC during manipulation, yielding Experimental Groups 2 (G2; 3.62 wt% of fibers) and 3 (G3; 7.24 wt% of fibers), which were compared against a control group containing no fibers (G1).
25627882	2	11	dep	Groups	352:357	arg1	3					390:390	3	390:390	3	390:390	Two concentrations of fibers were weighed and agglutinated into the GIC during manipulation, yielding Experimental Groups 2 (G2; 3.62 wt% of fibers) and 3 (G3; 7.24 wt% of fibers), which were compared against a control group containing no fibers (G1).
25627882	9	12	theme	cellulosic	1323:1332	arg1	fibers					1334:1339	The physically processed cellulosic fibers	1298:1339	The physically processed cellulosic fibers (especially at the higher concentration)	1298:1380	The physically processed cellulosic fibers (especially at the higher concentration) increased the compressive and diametral tensile strengths of the GIC, and demonstrated acceptable elastic modulus and stiffness.
25627882	6	13	theme	X-ray	1021:1025	arg1	EDS					1041:1043	EDS	1041:1043	EDS	1041:1043	The materials were then characterized by scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS).
25627882	6	13	theme	X-ray	1021:1025	arg1	spectroscopy					1027:1038	energy dispersive X-ray spectroscopy	1003:1038	energy dispersive X-ray spectroscopy (EDS)	1003:1044	The materials were then characterized by scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS).
25627882	6	14	theme	electron	973:980	arg1	SEM					994:996	SEM	994:996	SEM	994:996	The materials were then characterized by scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS).
25627882	6	14	theme	electron	973:980	arg1	microscopy					982:991	scanning electron microscopy	964:991	scanning electron microscopy (SEM)	964:997	The materials were then characterized by scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS).
25627882	2	15	dep	G2	362:363	arg1	fibers					378:383	fibers	378:383	fibers	378:383	Two concentrations of fibers were weighed and agglutinated into the GIC during manipulation, yielding Experimental Groups 2 (G2; 3.62 wt% of fibers) and 3 (G3; 7.24 wt% of fibers), which were compared against a control group containing no fibers (G1).
25627882	2	15	dep	G2	362:363	arg1	%					373:373	3.62 wt%	366:373	3.62 wt% of fibers	366:383	Two concentrations of fibers were weighed and agglutinated into the GIC during manipulation, yielding Experimental Groups 2 (G2; 3.62 wt% of fibers) and 3 (G3; 7.24 wt% of fibers), which were compared against a control group containing no fibers (G1).
25627882	5	16	theme	significant	759:769	arg1	differences					771:781	Statistically significant differences	745:781	Statistically significant differences in elastic modulus	745:800	Statistically significant differences in elastic modulus were also found between G2 and G1 and between G2 and G3, whereas the stiffness significantly differed between G1 and G2.
25627882	2	17	dep	3	390:390	arg1	G3					393:394	G3	393:394	G3	393:394	Two concentrations of fibers were weighed and agglutinated into the GIC during manipulation, yielding Experimental Groups 2 (G2; 3.62 wt% of fibers) and 3 (G3; 7.24 wt% of fibers), which were compared against a control group containing no fibers (G1).
25627882	8	18	theme	composites	1223:1232	arg1	spectra					1208:1214	The EDS spectra	1200:1214	The EDS spectra of the composites	1200:1232	The EDS spectra of the composites revealed the elemental compositions of the precursor materials.
25627882	2	19	contain	containing	462:471	arg2	G1					484:485	G1	484:485	G1	484:485	Two concentrations of fibers were weighed and agglutinated into the GIC during manipulation, yielding Experimental Groups 2 (G2; 3.62 wt% of fibers) and 3 (G3; 7.24 wt% of fibers), which were compared against a control group containing no fibers (G1).
25627882	2	19	contain	containing	462:471	arg1	group					456:460	a control group	446:460	a control group containing no fibers (G1)	446:486	Two concentrations of fibers were weighed and agglutinated into the GIC during manipulation, yielding Experimental Groups 2 (G2; 3.62 wt% of fibers) and 3 (G3; 7.24 wt% of fibers), which were compared against a control group containing no fibers (G1).
25627882	2	19	contain	containing	462:471	arg2	fibers					476:481	no fibers	473:481	no fibers (G1)	473:486	Two concentrations of fibers were weighed and agglutinated into the GIC during manipulation, yielding Experimental Groups 2 (G2; 3.62 wt% of fibers) and 3 (G3; 7.24 wt% of fibers), which were compared against a control group containing no fibers (G1).
25627882	2	20	theme	wt	371:372	arg1	fibers					378:383	fibers	378:383	fibers	378:383	Two concentrations of fibers were weighed and agglutinated into the GIC during manipulation, yielding Experimental Groups 2 (G2; 3.62 wt% of fibers) and 3 (G3; 7.24 wt% of fibers), which were compared against a control group containing no fibers (G1).
25627882	2	20	theme	wt	371:372	arg1	%					373:373	3.62 wt%	366:373	3.62 wt% of fibers	366:383	Two concentrations of fibers were weighed and agglutinated into the GIC during manipulation, yielding Experimental Groups 2 (G2; 3.62 wt% of fibers) and 3 (G3; 7.24 wt% of fibers), which were compared against a control group containing no fibers (G1).
25627882	7	21	theme	shaped	1063:1068	arg1	particles					1070:1078	Heterogeneously shaped particles	1047:1078	Heterogeneously shaped particles	1047:1078	Heterogeneously shaped particles were found on the G2 and G3 surfaces, and the cement matrices were randomly interspersed with long intermingled fibers.
25627882	2	22	theme	fibers	409:414	arg1	fibers					409:414	fibers	409:414	fibers	409:414	Two concentrations of fibers were weighed and agglutinated into the GIC during manipulation, yielding Experimental Groups 2 (G2; 3.62 wt% of fibers) and 3 (G3; 7.24 wt% of fibers), which were compared against a control group containing no fibers (G1).
25627882	2	22	theme	fibers	409:414	arg1	%					404:404	7.24 wt%	397:404	7.24 wt% of fibers	397:414	Two concentrations of fibers were weighed and agglutinated into the GIC during manipulation, yielding Experimental Groups 2 (G2; 3.62 wt% of fibers) and 3 (G3; 7.24 wt% of fibers), which were compared against a control group containing no fibers (G1).
25627882	0	23	dep	properties	83:92	arg1	Addition					0:7	Addition	0:7	Addition of mechanically processed cellulosic fibers to ionomer cement	0:69	Addition of mechanically processed cellulosic fibers to ionomer cement: mechanical properties.
25627882	9	24	theme	higher	1360:1365	arg1	concentration					1367:1379	the higher concentration	1356:1379	the higher concentration	1356:1379	The physically processed cellulosic fibers (especially at the higher concentration) increased the compressive and diametral tensile strengths of the GIC, and demonstrated acceptable elastic modulus and stiffness.
25627882	2	25	dep	G3	393:394	arg1	fibers					409:414	fibers	409:414	fibers	409:414	Two concentrations of fibers were weighed and agglutinated into the GIC during manipulation, yielding Experimental Groups 2 (G2; 3.62 wt% of fibers) and 3 (G3; 7.24 wt% of fibers), which were compared against a control group containing no fibers (G1).
25627882	2	25	dep	G3	393:394	arg1	%					404:404	7.24 wt%	397:404	7.24 wt% of fibers	397:414	Two concentrations of fibers were weighed and agglutinated into the GIC during manipulation, yielding Experimental Groups 2 (G2; 3.62 wt% of fibers) and 3 (G3; 7.24 wt% of fibers), which were compared against a control group containing no fibers (G1).
25627882	9	26	theme	acceptable	1469:1478	arg1	modulus					1488:1494	acceptable elastic modulus	1469:1494	acceptable elastic modulus	1469:1494	The physically processed cellulosic fibers (especially at the higher concentration) increased the compressive and diametral tensile strengths of the GIC, and demonstrated acceptable elastic modulus and stiffness.
25627882	3	27	dep	strengths	505:513	arg1	The					489:491	The	489:491	The	489:491	The compressive strengths and elastic modulus of the three groups, and their diametral tensile strengths and stiffness, were evaluated on a universal test machine.
25627882	0	28	theme	processed	25:33	arg1	fibers					46:51	mechanically processed cellulosic fibers	12:51	mechanically processed cellulosic fibers	12:51	Addition of mechanically processed cellulosic fibers to ionomer cement: mechanical properties.
25627882	9	29	theme	elastic	1480:1486	arg1	modulus					1488:1494	acceptable elastic modulus	1469:1494	acceptable elastic modulus	1469:1494	The physically processed cellulosic fibers (especially at the higher concentration) increased the compressive and diametral tensile strengths of the GIC, and demonstrated acceptable elastic modulus and stiffness.
25627882	8	30	theme	precursor	1277:1285	arg1	materials					1287:1295	the precursor materials	1273:1295	the precursor materials	1273:1295	The EDS spectra of the composites revealed the elemental compositions of the precursor materials.
25627882	7	31	theme	intermingled	1179:1190	arg1	fibers					1192:1197	long intermingled fibers	1174:1197	long intermingled fibers	1174:1197	Heterogeneously shaped particles were found on the G2 and G3 surfaces, and the cement matrices were randomly interspersed with long intermingled fibers.
25627882	3	32	theme	universal	629:637	arg1	machine					644:650	a universal test machine	627:650	a universal test machine	627:650	The compressive strengths and elastic modulus of the three groups, and their diametral tensile strengths and stiffness, were evaluated on a universal test machine.
25627882	4	33	theme	diametral	673:681	arg1	strengths					691:699	The compressive and diametral tensile strengths	653:699	The compressive and diametral tensile strengths	653:699	The compressive and diametral tensile strengths were significantly higher in G3 than in G1.
25627882	4	33	theme	diametral	673:681	arg1	higher					720:725	higher	720:725	higher	720:725	The compressive and diametral tensile strengths were significantly higher in G3 than in G1.
25627882	3	34	theme	groups	548:553	arg1	stiffness					598:606	stiffness	598:606	stiffness	598:606	The compressive strengths and elastic modulus of the three groups, and their diametral tensile strengths and stiffness, were evaluated on a universal test machine.
25627882	3	34	theme	groups	548:553	arg1	strengths					584:592	their diametral tensile strengths	560:592	their diametral tensile strengths	560:592	The compressive strengths and elastic modulus of the three groups, and their diametral tensile strengths and stiffness, were evaluated on a universal test machine.
25627882	3	34	theme	groups	548:553	arg1	strengths					505:513	compressive strengths	493:513	compressive strengths	493:513	The compressive strengths and elastic modulus of the three groups, and their diametral tensile strengths and stiffness, were evaluated on a universal test machine.
25627882	3	34	theme	groups	548:553	arg1	modulus					527:533	elastic modulus	519:533	elastic modulus	519:533	The compressive strengths and elastic modulus of the three groups, and their diametral tensile strengths and stiffness, were evaluated on a universal test machine.
25627882	8	35	theme	EDS	1204:1206	arg1	spectra					1208:1214	The EDS spectra	1200:1214	The EDS spectra of the composites	1200:1232	The EDS spectra of the composites revealed the elemental compositions of the precursor materials.
25627882	9	36	theme	diametral	1412:1420	arg1	strengths					1430:1438	the compressive and diametral tensile strengths	1392:1438	the compressive and diametral tensile strengths of the GIC	1392:1449	The physically processed cellulosic fibers (especially at the higher concentration) increased the compressive and diametral tensile strengths of the GIC, and demonstrated acceptable elastic modulus and stiffness.
25627882	0	37	theme	fibers	46:51	arg1	Addition					0:7	Addition	0:7	Addition of mechanically processed cellulosic fibers to ionomer cement	0:69	Addition of mechanically processed cellulosic fibers to ionomer cement: mechanical properties.
25627882	3	38	theme	test	639:642	arg1	machine					644:650	a universal test machine	627:650	a universal test machine	627:650	The compressive strengths and elastic modulus of the three groups, and their diametral tensile strengths and stiffness, were evaluated on a universal test machine.
25627882	3	39	theme	compressive	493:503	arg1	stiffness					598:606	stiffness	598:606	stiffness	598:606	The compressive strengths and elastic modulus of the three groups, and their diametral tensile strengths and stiffness, were evaluated on a universal test machine.
25627882	3	39	theme	compressive	493:503	arg1	strengths					584:592	their diametral tensile strengths	560:592	their diametral tensile strengths	560:592	The compressive strengths and elastic modulus of the three groups, and their diametral tensile strengths and stiffness, were evaluated on a universal test machine.
25627882	3	39	theme	compressive	493:503	arg1	strengths					505:513	compressive strengths	493:513	compressive strengths	493:513	The compressive strengths and elastic modulus of the three groups, and their diametral tensile strengths and stiffness, were evaluated on a universal test machine.
25627882	5	40	theme	elastic	786:792	arg1	modulus					794:800	elastic modulus	786:800	elastic modulus	786:800	Statistically significant differences in elastic modulus were also found between G2 and G1 and between G2 and G3, whereas the stiffness significantly differed between G1 and G2.
25627882	2	41	theme	fibers	378:383	arg1	fibers					378:383	fibers	378:383	fibers	378:383	Two concentrations of fibers were weighed and agglutinated into the GIC during manipulation, yielding Experimental Groups 2 (G2; 3.62 wt% of fibers) and 3 (G3; 7.24 wt% of fibers), which were compared against a control group containing no fibers (G1).
25627882	2	41	theme	fibers	378:383	arg1	%					373:373	3.62 wt%	366:373	3.62 wt% of fibers	366:383	Two concentrations of fibers were weighed and agglutinated into the GIC during manipulation, yielding Experimental Groups 2 (G2; 3.62 wt% of fibers) and 3 (G3; 7.24 wt% of fibers), which were compared against a control group containing no fibers (G1).
25627882	0	42	theme	cellulosic	35:44	arg1	fibers					46:51	mechanically processed cellulosic fibers	12:51	mechanically processed cellulosic fibers	12:51	Addition of mechanically processed cellulosic fibers to ionomer cement: mechanical properties.
25627882	2	43	theme	control	448:454	arg1	group					456:460	a control group	446:460	a control group containing no fibers (G1)	446:486	Two concentrations of fibers were weighed and agglutinated into the GIC during manipulation, yielding Experimental Groups 2 (G2; 3.62 wt% of fibers) and 3 (G3; 7.24 wt% of fibers), which were compared against a control group containing no fibers (G1).
25627882	9	44	from	concentration	1367:1379	arg1	fibers					1334:1339	The physically processed cellulosic fibers	1298:1339	The physically processed cellulosic fibers (especially at the higher concentration)	1298:1380	The physically processed cellulosic fibers (especially at the higher concentration) increased the compressive and diametral tensile strengths of the GIC, and demonstrated acceptable elastic modulus and stiffness.
25627882	0	45	theme	ionomer	56:62	arg1	cement					64:69	ionomer cement	56:69	ionomer cement	56:69	Addition of mechanically processed cellulosic fibers to ionomer cement: mechanical properties.
25627882	6	46	theme	energy	1003:1008	arg1	EDS					1041:1043	EDS	1041:1043	EDS	1041:1043	The materials were then characterized by scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS).
25627882	6	46	theme	energy	1003:1008	arg1	spectroscopy					1027:1038	energy dispersive X-ray spectroscopy	1003:1038	energy dispersive X-ray spectroscopy (EDS)	1003:1044	The materials were then characterized by scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS).
25627882	2	47	theme	Experimental	339:350	arg1	Groups					352:357	Experimental Groups 2 (G2; 3.62 wt% of fibers) and 3 (G3; 7.24 wt% of fibers)	339:415	Experimental Groups 2 (G2; 3.62 wt% of fibers) and 3 (G3; 7.24 wt% of fibers)	339:415	Two concentrations of fibers were weighed and agglutinated into the GIC during manipulation, yielding Experimental Groups 2 (G2; 3.62 wt% of fibers) and 3 (G3; 7.24 wt% of fibers), which were compared against a control group containing no fibers (G1).
25627882	3	48	theme	diametral	566:574	arg1	strengths					584:592	their diametral tensile strengths	560:592	their diametral tensile strengths	560:592	The compressive strengths and elastic modulus of the three groups, and their diametral tensile strengths and stiffness, were evaluated on a universal test machine.
25627882	3	48	theme	diametral	566:574	arg1	strengths					505:513	compressive strengths	493:513	compressive strengths	493:513	The compressive strengths and elastic modulus of the three groups, and their diametral tensile strengths and stiffness, were evaluated on a universal test machine.
25627882	8	49	theme	materials	1287:1295	arg1	compositions					1257:1268	the elemental compositions	1243:1268	the elemental compositions of the precursor materials	1243:1295	The EDS spectra of the composites revealed the elemental compositions of the precursor materials.
25627882	7	50	located	found	1085:1089	arg2	particles					1070:1078	Heterogeneously shaped particles	1047:1078	Heterogeneously shaped particles	1047:1078	Heterogeneously shaped particles were found on the G2 and G3 surfaces, and the cement matrices were randomly interspersed with long intermingled fibers.
25627882	7	50	located	found	1085:1089	arg1	surfaces					1108:1115	the G2 and G3 surfaces	1094:1115	the G2 and G3 surfaces	1094:1115	Heterogeneously shaped particles were found on the G2 and G3 surfaces, and the cement matrices were randomly interspersed with long intermingled fibers.
25627882	1	51	theme	conventional	110:121	arg1	cement					149:154	conventional restorative glass ionomer cement	110:154	conventional restorative glass ionomer cement (GIC)	110:160	In this study, conventional restorative glass ionomer cement (GIC) was modified by embedding it with mechanically processed cellulose fibers.
25627882	1	51	theme	conventional	110:121	arg1	GIC					157:159	GIC	157:159	GIC	157:159	In this study, conventional restorative glass ionomer cement (GIC) was modified by embedding it with mechanically processed cellulose fibers.
25627882	3	52	theme	tensile	576:582	arg1	strengths					584:592	their diametral tensile strengths	560:592	their diametral tensile strengths	560:592	The compressive strengths and elastic modulus of the three groups, and their diametral tensile strengths and stiffness, were evaluated on a universal test machine.
25627882	3	52	theme	tensile	576:582	arg1	strengths					505:513	compressive strengths	493:513	compressive strengths	493:513	The compressive strengths and elastic modulus of the three groups, and their diametral tensile strengths and stiffness, were evaluated on a universal test machine.
25627882	2	53	theme	fibers	259:264	arg1	concentrations					241:254	Two concentrations	237:254	Two concentrations of fibers	237:264	Two concentrations of fibers were weighed and agglutinated into the GIC during manipulation, yielding Experimental Groups 2 (G2; 3.62 wt% of fibers) and 3 (G3; 7.24 wt% of fibers), which were compared against a control group containing no fibers (G1).
25627882	9	54	theme	tensile	1422:1428	arg1	strengths					1430:1438	the compressive and diametral tensile strengths	1392:1438	the compressive and diametral tensile strengths of the GIC	1392:1449	The physically processed cellulosic fibers (especially at the higher concentration) increased the compressive and diametral tensile strengths of the GIC, and demonstrated acceptable elastic modulus and stiffness.
25627882	1	55	theme	restorative	123:133	arg1	cement					149:154	conventional restorative glass ionomer cement	110:154	conventional restorative glass ionomer cement (GIC)	110:160	In this study, conventional restorative glass ionomer cement (GIC) was modified by embedding it with mechanically processed cellulose fibers.
25627882	1	55	theme	restorative	123:133	arg1	GIC					157:159	GIC	157:159	GIC	157:159	In this study, conventional restorative glass ionomer cement (GIC) was modified by embedding it with mechanically processed cellulose fibers.
25627882	1	56	theme	processed	209:217	arg1	fibers					229:234	mechanically processed cellulose fibers	196:234	mechanically processed cellulose fibers	196:234	In this study, conventional restorative glass ionomer cement (GIC) was modified by embedding it with mechanically processed cellulose fibers.
25627882	4	57	theme	compressive	657:667	arg1	strengths					691:699	The compressive and diametral tensile strengths	653:699	The compressive and diametral tensile strengths	653:699	The compressive and diametral tensile strengths were significantly higher in G3 than in G1.
25627882	4	57	theme	compressive	657:667	arg1	higher					720:725	higher	720:725	higher	720:725	The compressive and diametral tensile strengths were significantly higher in G3 than in G1.
25627882	1	58	theme	glass	135:139	arg1	cement					149:154	conventional restorative glass ionomer cement	110:154	conventional restorative glass ionomer cement (GIC)	110:160	In this study, conventional restorative glass ionomer cement (GIC) was modified by embedding it with mechanically processed cellulose fibers.
25627882	1	58	theme	glass	135:139	arg1	GIC					157:159	GIC	157:159	GIC	157:159	In this study, conventional restorative glass ionomer cement (GIC) was modified by embedding it with mechanically processed cellulose fibers.
25627882	1	59	theme	cellulose	219:227	arg1	fibers					229:234	mechanically processed cellulose fibers	196:234	mechanically processed cellulose fibers	196:234	In this study, conventional restorative glass ionomer cement (GIC) was modified by embedding it with mechanically processed cellulose fibers.
25627882	7	60	theme	G3	1105:1106	arg1	surfaces					1108:1115	the G2 and G3 surfaces	1094:1115	the G2 and G3 surfaces	1094:1115	Heterogeneously shaped particles were found on the G2 and G3 surfaces, and the cement matrices were randomly interspersed with long intermingled fibers.
25627882	1	61	theme	ionomer	141:147	arg1	cement					149:154	conventional restorative glass ionomer cement	110:154	conventional restorative glass ionomer cement (GIC)	110:160	In this study, conventional restorative glass ionomer cement (GIC) was modified by embedding it with mechanically processed cellulose fibers.
25627882	1	61	theme	ionomer	141:147	arg1	GIC					157:159	GIC	157:159	GIC	157:159	In this study, conventional restorative glass ionomer cement (GIC) was modified by embedding it with mechanically processed cellulose fibers.
25627882	6	62	theme	scanning	964:971	arg1	SEM					994:996	SEM	994:996	SEM	994:996	The materials were then characterized by scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS).
25627882	6	62	theme	scanning	964:971	arg1	microscopy					982:991	scanning electron microscopy	964:991	scanning electron microscopy (SEM)	964:997	The materials were then characterized by scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS).
25627882	2	63	theme	wt	402:403	arg1	fibers					409:414	fibers	409:414	fibers	409:414	Two concentrations of fibers were weighed and agglutinated into the GIC during manipulation, yielding Experimental Groups 2 (G2; 3.62 wt% of fibers) and 3 (G3; 7.24 wt% of fibers), which were compared against a control group containing no fibers (G1).
25627882	2	63	theme	wt	402:403	arg1	%					404:404	7.24 wt%	397:404	7.24 wt% of fibers	397:414	Two concentrations of fibers were weighed and agglutinated into the GIC during manipulation, yielding Experimental Groups 2 (G2; 3.62 wt% of fibers) and 3 (G3; 7.24 wt% of fibers), which were compared against a control group containing no fibers (G1).
25627882	3	64	theme	elastic	519:525	arg1	modulus					527:533	elastic modulus	519:533	elastic modulus	519:533	The compressive strengths and elastic modulus of the three groups, and their diametral tensile strengths and stiffness, were evaluated on a universal test machine.
25627882	7	65	theme	cement	1126:1131	arg1	matrices					1133:1140	the cement matrices	1122:1140	the cement matrices	1122:1140	Heterogeneously shaped particles were found on the G2 and G3 surfaces, and the cement matrices were randomly interspersed with long intermingled fibers.
28153461	2	0	theme	chondroitin	475:485	arg1	backbone					441:448	The backbone	437:448	The backbone of HmG	437:455	The backbone of HmG was identified as chondroitin 6-O sulfate, while the major O-4 sulfated fucose branches linked to O-3 position of glucuronic acid in almost every disaccharide unit.
28153461	2	0	theme	chondroitin	475:485	arg1	sulfate					491:497	chondroitin 6-O sulfate	475:497	chondroitin 6-O sulfate	475:497	The backbone of HmG was identified as chondroitin 6-O sulfate, while the major O-4 sulfated fucose branches linked to O-3 position of glucuronic acid in almost every disaccharide unit.
28153461	3	1	theme	molecular	715:723	arg1	heparin					732:738	low molecular weight heparin	711:738	low molecular weight heparin	711:738	The anticoagulant activities of HmG and DHmG were assessed and compared with heparin and low molecular weight heparin.
28153461	0	2	theme	sulfate	98:104	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification, structural characterization and anticoagulant properties of fucosylated chondroitin sulfate isolated from Holothuria mexicana.
28153461	0	2	theme	sulfate	98:104	arg1	characterization					25:40	structural characterization	14:40	structural characterization	14:40	Purification, structural characterization and anticoagulant properties of fucosylated chondroitin sulfate isolated from Holothuria mexicana.
28153461	0	2	theme	sulfate	98:104	arg1	properties					60:69	anticoagulant properties	46:69	anticoagulant properties	46:69	Purification, structural characterization and anticoagulant properties of fucosylated chondroitin sulfate isolated from Holothuria mexicana.
28153461	1	3	theme	NMR	352:354	arg1	spectrum					356:363	NMR spectrum	352:363	NMR spectrum	352:363	A novel fucosylated chondroitin sulfate (HmG) was isolated from sea cucumber Holothuria mexicana, the structure of which was characterized by monosaccharide composition, disaccharide composition, IR, 1H and 13C NMR spectrum, additionally with two dimensional NMR spectrum of degraded HmG (DHmG).
28153461	2	4	theme	acid	582:585	arg1	position					559:566	O-3 position	555:566	O-3 position of glucuronic acid in almost every disaccharide unit	555:619	The backbone of HmG was identified as chondroitin 6-O sulfate, while the major O-4 sulfated fucose branches linked to O-3 position of glucuronic acid in almost every disaccharide unit.
28153461	5	5	theme	anticoagulant	1041:1053	arg1	drug					1055:1058	a safer anticoagulant drug	1033:1058	a safer anticoagulant drug	1033:1058	DHmG showed similar anticoagulant properties to low molecular weight heparin with less bleeding risks, making it a safer anticoagulant drug.
28153461	1	6	theme	dimensional	388:398	arg1	spectrum					404:411	two dimensional NMR spectrum	384:411	two dimensional NMR spectrum of degraded HmG (DHmG)	384:434	A novel fucosylated chondroitin sulfate (HmG) was isolated from sea cucumber Holothuria mexicana, the structure of which was characterized by monosaccharide composition, disaccharide composition, IR, 1H and 13C NMR spectrum, additionally with two dimensional NMR spectrum of degraded HmG (DHmG).
28153461	2	7	theme	major	510:514	arg1	branches					536:543	the major O-4 sulfated fucose branches	506:543	the major O-4 sulfated fucose branches	506:543	The backbone of HmG was identified as chondroitin 6-O sulfate, while the major O-4 sulfated fucose branches linked to O-3 position of glucuronic acid in almost every disaccharide unit.
28153461	3	8	theme	anticoagulant	626:638	arg1	activities					640:649	The anticoagulant activities	622:649	The anticoagulant activities of HmG and DHmG	622:665	The anticoagulant activities of HmG and DHmG were assessed and compared with heparin and low molecular weight heparin.
28153461	2	9	theme	glucuronic	571:580	arg1	acid					582:585	glucuronic acid	571:585	glucuronic acid	571:585	The backbone of HmG was identified as chondroitin 6-O sulfate, while the major O-4 sulfated fucose branches linked to O-3 position of glucuronic acid in almost every disaccharide unit.
28153461	0	10	gly	fucosylated	74:84	arg1	sulfate					98:104	fucosylated chondroitin sulfate	74:104	fucosylated chondroitin sulfate	74:104	Purification, structural characterization and anticoagulant properties of fucosylated chondroitin sulfate isolated from Holothuria mexicana.
28153461	2	11	from	position	559:566	arg1	unit					616:619	almost every disaccharide unit	590:619	almost every disaccharide unit	590:619	The backbone of HmG was identified as chondroitin 6-O sulfate, while the major O-4 sulfated fucose branches linked to O-3 position of glucuronic acid in almost every disaccharide unit.
28153461	1	12	theme	monosaccharide	283:296	arg1	IR					337:338	IR	337:338	IR	337:338	A novel fucosylated chondroitin sulfate (HmG) was isolated from sea cucumber Holothuria mexicana, the structure of which was characterized by monosaccharide composition, disaccharide composition, IR, 1H and 13C NMR spectrum, additionally with two dimensional NMR spectrum of degraded HmG (DHmG).
28153461	1	12	theme	monosaccharide	283:296	arg1	composition					298:308	monosaccharide composition	283:308	monosaccharide composition	283:308	A novel fucosylated chondroitin sulfate (HmG) was isolated from sea cucumber Holothuria mexicana, the structure of which was characterized by monosaccharide composition, disaccharide composition, IR, 1H and 13C NMR spectrum, additionally with two dimensional NMR spectrum of degraded HmG (DHmG).
28153461	1	12	theme	monosaccharide	283:296	arg1	composition					324:334	disaccharide composition	311:334	disaccharide composition	311:334	A novel fucosylated chondroitin sulfate (HmG) was isolated from sea cucumber Holothuria mexicana, the structure of which was characterized by monosaccharide composition, disaccharide composition, IR, 1H and 13C NMR spectrum, additionally with two dimensional NMR spectrum of degraded HmG (DHmG).
28153461	1	12	theme	monosaccharide	283:296	arg1	13C					348:350	13C	348:350	13C	348:350	A novel fucosylated chondroitin sulfate (HmG) was isolated from sea cucumber Holothuria mexicana, the structure of which was characterized by monosaccharide composition, disaccharide composition, IR, 1H and 13C NMR spectrum, additionally with two dimensional NMR spectrum of degraded HmG (DHmG).
28153461	1	12	theme	monosaccharide	283:296	arg1	1H					341:342	1H	341:342	1H	341:342	A novel fucosylated chondroitin sulfate (HmG) was isolated from sea cucumber Holothuria mexicana, the structure of which was characterized by monosaccharide composition, disaccharide composition, IR, 1H and 13C NMR spectrum, additionally with two dimensional NMR spectrum of degraded HmG (DHmG).
28153461	1	13	dep	1H	341:342	arg1	spectrum					356:363	NMR spectrum	352:363	NMR spectrum	352:363	A novel fucosylated chondroitin sulfate (HmG) was isolated from sea cucumber Holothuria mexicana, the structure of which was characterized by monosaccharide composition, disaccharide composition, IR, 1H and 13C NMR spectrum, additionally with two dimensional NMR spectrum of degraded HmG (DHmG).
28153461	4	14	theme	partial	828:834	arg1	time					852:855	the activated partial thrombo-plastin time	814:855	the activated partial thrombo-plastin time	814:855	The results indicated that HmG and DHmG both could significantly prolong the activated partial thrombo-plastin time, and the properties were well related to its molecular weight.
28153461	0	15	dep	Holothuria	120:129	arg1	mexicana					131:138	Holothuria mexicana	120:138	Holothuria mexicana	120:138	Purification, structural characterization and anticoagulant properties of fucosylated chondroitin sulfate isolated from Holothuria mexicana.
28153461	2	16	theme	disaccharide	603:614	arg1	unit					616:619	almost every disaccharide unit	590:619	almost every disaccharide unit	590:619	The backbone of HmG was identified as chondroitin 6-O sulfate, while the major O-4 sulfated fucose branches linked to O-3 position of glucuronic acid in almost every disaccharide unit.
28153461	5	17	theme	molecular	972:980	arg1	heparin					989:995	low molecular weight heparin	968:995	low molecular weight heparin with less bleeding risks	968:1020	DHmG showed similar anticoagulant properties to low molecular weight heparin with less bleeding risks, making it a safer anticoagulant drug.
28153461	2	18	attach	linked	545:550	arg2	branches					536:543	the major O-4 sulfated fucose branches	506:543	the major O-4 sulfated fucose branches	506:543	The backbone of HmG was identified as chondroitin 6-O sulfate, while the major O-4 sulfated fucose branches linked to O-3 position of glucuronic acid in almost every disaccharide unit.
28153461	2	18	attach	linked	545:550	arg1	position					559:566	O-3 position	555:566	O-3 position of glucuronic acid in almost every disaccharide unit	555:619	The backbone of HmG was identified as chondroitin 6-O sulfate, while the major O-4 sulfated fucose branches linked to O-3 position of glucuronic acid in almost every disaccharide unit.
28153461	5	19	with	heparin	989:995	arg1	risks					1016:1020	less bleeding risks	1002:1020	less bleeding risks	1002:1020	DHmG showed similar anticoagulant properties to low molecular weight heparin with less bleeding risks, making it a safer anticoagulant drug.
28153461	2	20	theme	every	597:601	arg1	unit					616:619	almost every disaccharide unit	590:619	almost every disaccharide unit	590:619	The backbone of HmG was identified as chondroitin 6-O sulfate, while the major O-4 sulfated fucose branches linked to O-3 position of glucuronic acid in almost every disaccharide unit.
28153461	3	21	theme	low	711:713	arg1	heparin					732:738	low molecular weight heparin	711:738	low molecular weight heparin	711:738	The anticoagulant activities of HmG and DHmG were assessed and compared with heparin and low molecular weight heparin.
28153461	4	22	theme	thrombo-plastin	836:850	arg1	time					852:855	the activated partial thrombo-plastin time	814:855	the activated partial thrombo-plastin time	814:855	The results indicated that HmG and DHmG both could significantly prolong the activated partial thrombo-plastin time, and the properties were well related to its molecular weight.
28153461	1	23	theme	sea	205:207	arg1	cucumber					209:216	sea cucumber Holothuria mexicana, the structure of which was characterized by monosaccharide composition, disaccharide composition, IR, 1H and 13C NMR spectrum, additionally with two dimensional NMR spectrum of degraded HmG (DHmG)	205:434	cucumber	209:216	A novel fucosylated chondroitin sulfate (HmG) was isolated from sea cucumber Holothuria mexicana, the structure of which was characterized by monosaccharide composition, disaccharide composition, IR, 1H and 13C NMR spectrum, additionally with two dimensional NMR spectrum of degraded HmG (DHmG).
28153461	4	24	theme	molecular	902:910	arg1	weight					912:917	its molecular weight	898:917	its molecular weight	898:917	The results indicated that HmG and DHmG both could significantly prolong the activated partial thrombo-plastin time, and the properties were well related to its molecular weight.
28153461	5	25	theme	similar	932:938	arg1	properties					954:963	similar anticoagulant properties	932:963	similar anticoagulant properties	932:963	DHmG showed similar anticoagulant properties to low molecular weight heparin with less bleeding risks, making it a safer anticoagulant drug.
28153461	0	26	theme	structural	14:23	arg1	characterization					25:40	structural characterization	14:40	structural characterization	14:40	Purification, structural characterization and anticoagulant properties of fucosylated chondroitin sulfate isolated from Holothuria mexicana.
28153461	1	27	theme	disaccharide	311:322	arg1	composition					298:308	monosaccharide composition	283:308	monosaccharide composition	283:308	A novel fucosylated chondroitin sulfate (HmG) was isolated from sea cucumber Holothuria mexicana, the structure of which was characterized by monosaccharide composition, disaccharide composition, IR, 1H and 13C NMR spectrum, additionally with two dimensional NMR spectrum of degraded HmG (DHmG).
28153461	1	27	theme	disaccharide	311:322	arg1	composition					324:334	disaccharide composition	311:334	disaccharide composition	311:334	A novel fucosylated chondroitin sulfate (HmG) was isolated from sea cucumber Holothuria mexicana, the structure of which was characterized by monosaccharide composition, disaccharide composition, IR, 1H and 13C NMR spectrum, additionally with two dimensional NMR spectrum of degraded HmG (DHmG).
28153461	2	28	theme	fucose	529:534	arg1	branches					536:543	the major O-4 sulfated fucose branches	506:543	the major O-4 sulfated fucose branches	506:543	The backbone of HmG was identified as chondroitin 6-O sulfate, while the major O-4 sulfated fucose branches linked to O-3 position of glucuronic acid in almost every disaccharide unit.
28153461	1	29	attach	isolated	191:198	arg2	HmG					182:184	HmG	182:184	HmG	182:184	A novel fucosylated chondroitin sulfate (HmG) was isolated from sea cucumber Holothuria mexicana, the structure of which was characterized by monosaccharide composition, disaccharide composition, IR, 1H and 13C NMR spectrum, additionally with two dimensional NMR spectrum of degraded HmG (DHmG).
28153461	1	29	attach	isolated	191:198	arg2	sulfate					173:179	A novel fucosylated chondroitin sulfate	141:179	A novel fucosylated chondroitin sulfate (HmG)	141:185	A novel fucosylated chondroitin sulfate (HmG) was isolated from sea cucumber Holothuria mexicana, the structure of which was characterized by monosaccharide composition, disaccharide composition, IR, 1H and 13C NMR spectrum, additionally with two dimensional NMR spectrum of degraded HmG (DHmG).
28153461	1	29	attach	isolated	191:198	arg1	cucumber					209:216	sea cucumber Holothuria mexicana, the structure of which was characterized by monosaccharide composition, disaccharide composition, IR, 1H and 13C NMR spectrum, additionally with two dimensional NMR spectrum of degraded HmG (DHmG)	205:434	cucumber	209:216	A novel fucosylated chondroitin sulfate (HmG) was isolated from sea cucumber Holothuria mexicana, the structure of which was characterized by monosaccharide composition, disaccharide composition, IR, 1H and 13C NMR spectrum, additionally with two dimensional NMR spectrum of degraded HmG (DHmG).
28153461	5	30	theme	anticoagulant	940:952	arg1	properties					954:963	similar anticoagulant properties	932:963	similar anticoagulant properties	932:963	DHmG showed similar anticoagulant properties to low molecular weight heparin with less bleeding risks, making it a safer anticoagulant drug.
28153461	1	31	theme	Holothuria	218:227	arg1	cucumber					209:216	sea cucumber Holothuria mexicana, the structure of which was characterized by monosaccharide composition, disaccharide composition, IR, 1H and 13C NMR spectrum, additionally with two dimensional NMR spectrum of degraded HmG (DHmG)	205:434	cucumber	209:216	A novel fucosylated chondroitin sulfate (HmG) was isolated from sea cucumber Holothuria mexicana, the structure of which was characterized by monosaccharide composition, disaccharide composition, IR, 1H and 13C NMR spectrum, additionally with two dimensional NMR spectrum of degraded HmG (DHmG).
28153461	0	32	theme	anticoagulant	46:58	arg1	properties					60:69	anticoagulant properties	46:69	anticoagulant properties	46:69	Purification, structural characterization and anticoagulant properties of fucosylated chondroitin sulfate isolated from Holothuria mexicana.
28153461	3	33	theme	weight	725:730	arg1	heparin					732:738	low molecular weight heparin	711:738	low molecular weight heparin	711:738	The anticoagulant activities of HmG and DHmG were assessed and compared with heparin and low molecular weight heparin.
28153461	2	34	theme	sulfated	520:527	arg1	branches					536:543	the major O-4 sulfated fucose branches	506:543	the major O-4 sulfated fucose branches	506:543	The backbone of HmG was identified as chondroitin 6-O sulfate, while the major O-4 sulfated fucose branches linked to O-3 position of glucuronic acid in almost every disaccharide unit.
28153461	1	35	theme	NMR	400:402	arg1	spectrum					404:411	two dimensional NMR spectrum	384:411	two dimensional NMR spectrum of degraded HmG (DHmG)	384:434	A novel fucosylated chondroitin sulfate (HmG) was isolated from sea cucumber Holothuria mexicana, the structure of which was characterized by monosaccharide composition, disaccharide composition, IR, 1H and 13C NMR spectrum, additionally with two dimensional NMR spectrum of degraded HmG (DHmG).
28153461	5	36	theme	bleeding	1007:1014	arg1	risks					1016:1020	less bleeding risks	1002:1020	less bleeding risks	1002:1020	DHmG showed similar anticoagulant properties to low molecular weight heparin with less bleeding risks, making it a safer anticoagulant drug.
28153461	4	37	dep	HmG	768:770	arg1	both					781:784	both	781:784	both	781:784	The results indicated that HmG and DHmG both could significantly prolong the activated partial thrombo-plastin time, and the properties were well related to its molecular weight.
28153461	1	38	gly	fucosylated	149:159	arg1	HmG					182:184	HmG	182:184	HmG	182:184	A novel fucosylated chondroitin sulfate (HmG) was isolated from sea cucumber Holothuria mexicana, the structure of which was characterized by monosaccharide composition, disaccharide composition, IR, 1H and 13C NMR spectrum, additionally with two dimensional NMR spectrum of degraded HmG (DHmG).
28153461	1	38	gly	fucosylated	149:159	arg1	sulfate					173:179	A novel fucosylated chondroitin sulfate	141:179	A novel fucosylated chondroitin sulfate (HmG)	141:185	A novel fucosylated chondroitin sulfate (HmG) was isolated from sea cucumber Holothuria mexicana, the structure of which was characterized by monosaccharide composition, disaccharide composition, IR, 1H and 13C NMR spectrum, additionally with two dimensional NMR spectrum of degraded HmG (DHmG).
28153461	2	39	theme	HmG	453:455	arg1	backbone					441:448	The backbone	437:448	The backbone of HmG	437:455	The backbone of HmG was identified as chondroitin 6-O sulfate, while the major O-4 sulfated fucose branches linked to O-3 position of glucuronic acid in almost every disaccharide unit.
28153461	2	39	theme	HmG	453:455	arg1	sulfate					491:497	chondroitin 6-O sulfate	475:497	chondroitin 6-O sulfate	475:497	The backbone of HmG was identified as chondroitin 6-O sulfate, while the major O-4 sulfated fucose branches linked to O-3 position of glucuronic acid in almost every disaccharide unit.
28153461	2	40	theme	6-O	487:489	arg1	backbone					441:448	The backbone	437:448	The backbone of HmG	437:455	The backbone of HmG was identified as chondroitin 6-O sulfate, while the major O-4 sulfated fucose branches linked to O-3 position of glucuronic acid in almost every disaccharide unit.
28153461	2	40	theme	6-O	487:489	arg1	sulfate					491:497	chondroitin 6-O sulfate	475:497	chondroitin 6-O sulfate	475:497	The backbone of HmG was identified as chondroitin 6-O sulfate, while the major O-4 sulfated fucose branches linked to O-3 position of glucuronic acid in almost every disaccharide unit.
28153461	2	41	theme	O-4	516:518	arg1	branches					536:543	the major O-4 sulfated fucose branches	506:543	the major O-4 sulfated fucose branches	506:543	The backbone of HmG was identified as chondroitin 6-O sulfate, while the major O-4 sulfated fucose branches linked to O-3 position of glucuronic acid in almost every disaccharide unit.
28153461	4	42	theme	activated	818:826	arg1	time					852:855	the activated partial thrombo-plastin time	814:855	the activated partial thrombo-plastin time	814:855	The results indicated that HmG and DHmG both could significantly prolong the activated partial thrombo-plastin time, and the properties were well related to its molecular weight.
28153461	2	43	theme	O-3	555:557	arg1	position					559:566	O-3 position	555:566	O-3 position of glucuronic acid in almost every disaccharide unit	555:619	The backbone of HmG was identified as chondroitin 6-O sulfate, while the major O-4 sulfated fucose branches linked to O-3 position of glucuronic acid in almost every disaccharide unit.
28153461	1	44	theme	degraded	416:423	arg1	HmG					425:427	degraded HmG	416:427	degraded HmG (DHmG)	416:434	A novel fucosylated chondroitin sulfate (HmG) was isolated from sea cucumber Holothuria mexicana, the structure of which was characterized by monosaccharide composition, disaccharide composition, IR, 1H and 13C NMR spectrum, additionally with two dimensional NMR spectrum of degraded HmG (DHmG).
28153461	1	44	theme	degraded	416:423	arg1	DHmG					430:433	DHmG	430:433	DHmG	430:433	A novel fucosylated chondroitin sulfate (HmG) was isolated from sea cucumber Holothuria mexicana, the structure of which was characterized by monosaccharide composition, disaccharide composition, IR, 1H and 13C NMR spectrum, additionally with two dimensional NMR spectrum of degraded HmG (DHmG).
28153461	3	45	theme	DHmG	662:665	arg1	activities					640:649	The anticoagulant activities	622:649	The anticoagulant activities of HmG and DHmG	622:665	The anticoagulant activities of HmG and DHmG were assessed and compared with heparin and low molecular weight heparin.
28153461	1	46	theme	novel	143:147	arg1	HmG					182:184	HmG	182:184	HmG	182:184	A novel fucosylated chondroitin sulfate (HmG) was isolated from sea cucumber Holothuria mexicana, the structure of which was characterized by monosaccharide composition, disaccharide composition, IR, 1H and 13C NMR spectrum, additionally with two dimensional NMR spectrum of degraded HmG (DHmG).
28153461	1	46	theme	novel	143:147	arg1	sulfate					173:179	A novel fucosylated chondroitin sulfate	141:179	A novel fucosylated chondroitin sulfate (HmG)	141:185	A novel fucosylated chondroitin sulfate (HmG) was isolated from sea cucumber Holothuria mexicana, the structure of which was characterized by monosaccharide composition, disaccharide composition, IR, 1H and 13C NMR spectrum, additionally with two dimensional NMR spectrum of degraded HmG (DHmG).
28153461	1	47	theme	HmG	425:427	arg1	spectrum					404:411	two dimensional NMR spectrum	384:411	two dimensional NMR spectrum of degraded HmG (DHmG)	384:434	A novel fucosylated chondroitin sulfate (HmG) was isolated from sea cucumber Holothuria mexicana, the structure of which was characterized by monosaccharide composition, disaccharide composition, IR, 1H and 13C NMR spectrum, additionally with two dimensional NMR spectrum of degraded HmG (DHmG).
28153461	5	48	theme	weight	982:987	arg1	heparin					989:995	low molecular weight heparin	968:995	low molecular weight heparin with less bleeding risks	968:1020	DHmG showed similar anticoagulant properties to low molecular weight heparin with less bleeding risks, making it a safer anticoagulant drug.
28153461	1	49	theme	fucosylated	149:159	arg1	HmG					182:184	HmG	182:184	HmG	182:184	A novel fucosylated chondroitin sulfate (HmG) was isolated from sea cucumber Holothuria mexicana, the structure of which was characterized by monosaccharide composition, disaccharide composition, IR, 1H and 13C NMR spectrum, additionally with two dimensional NMR spectrum of degraded HmG (DHmG).
28153461	1	49	theme	fucosylated	149:159	arg1	sulfate					173:179	A novel fucosylated chondroitin sulfate	141:179	A novel fucosylated chondroitin sulfate (HmG)	141:185	A novel fucosylated chondroitin sulfate (HmG) was isolated from sea cucumber Holothuria mexicana, the structure of which was characterized by monosaccharide composition, disaccharide composition, IR, 1H and 13C NMR spectrum, additionally with two dimensional NMR spectrum of degraded HmG (DHmG).
28153461	5	50	theme	safer	1035:1039	arg1	drug					1055:1058	a safer anticoagulant drug	1033:1058	a safer anticoagulant drug	1033:1058	DHmG showed similar anticoagulant properties to low molecular weight heparin with less bleeding risks, making it a safer anticoagulant drug.
28153461	5	51	theme	low	968:970	arg1	heparin					989:995	low molecular weight heparin	968:995	low molecular weight heparin with less bleeding risks	968:1020	DHmG showed similar anticoagulant properties to low molecular weight heparin with less bleeding risks, making it a safer anticoagulant drug.
28153461	1	52	theme	chondroitin	161:171	arg1	HmG					182:184	HmG	182:184	HmG	182:184	A novel fucosylated chondroitin sulfate (HmG) was isolated from sea cucumber Holothuria mexicana, the structure of which was characterized by monosaccharide composition, disaccharide composition, IR, 1H and 13C NMR spectrum, additionally with two dimensional NMR spectrum of degraded HmG (DHmG).
28153461	1	52	theme	chondroitin	161:171	arg1	sulfate					173:179	A novel fucosylated chondroitin sulfate	141:179	A novel fucosylated chondroitin sulfate (HmG)	141:185	A novel fucosylated chondroitin sulfate (HmG) was isolated from sea cucumber Holothuria mexicana, the structure of which was characterized by monosaccharide composition, disaccharide composition, IR, 1H and 13C NMR spectrum, additionally with two dimensional NMR spectrum of degraded HmG (DHmG).
28153461	0	53	theme	chondroitin	86:96	arg1	sulfate					98:104	fucosylated chondroitin sulfate	74:104	fucosylated chondroitin sulfate	74:104	Purification, structural characterization and anticoagulant properties of fucosylated chondroitin sulfate isolated from Holothuria mexicana.
28153461	3	54	theme	HmG	654:656	arg1	activities					640:649	The anticoagulant activities	622:649	The anticoagulant activities of HmG and DHmG	622:665	The anticoagulant activities of HmG and DHmG were assessed and compared with heparin and low molecular weight heparin.
28153461	1	55	dep	cucumber	209:216	arg1	mexicana					229:236	sea cucumber Holothuria mexicana, the structure of which was characterized by monosaccharide composition, disaccharide composition, IR, 1H and 13C NMR spectrum, additionally with two dimensional NMR spectrum of degraded HmG (DHmG)	205:434	mexicana	229:236	A novel fucosylated chondroitin sulfate (HmG) was isolated from sea cucumber Holothuria mexicana, the structure of which was characterized by monosaccharide composition, disaccharide composition, IR, 1H and 13C NMR spectrum, additionally with two dimensional NMR spectrum of degraded HmG (DHmG).
28153461	0	56	theme	fucosylated	74:84	arg1	sulfate					98:104	fucosylated chondroitin sulfate	74:104	fucosylated chondroitin sulfate	74:104	Purification, structural characterization and anticoagulant properties of fucosylated chondroitin sulfate isolated from Holothuria mexicana.
28153461	1	57	theme	which	256:260	arg1	structure					243:251	the structure	239:251	the structure	239:251	A novel fucosylated chondroitin sulfate (HmG) was isolated from sea cucumber Holothuria mexicana, the structure of which was characterized by monosaccharide composition, disaccharide composition, IR, 1H and 13C NMR spectrum, additionally with two dimensional NMR spectrum of degraded HmG (DHmG).
25924363	0	0	theme	laccase	95:101	arg1	immobilization					103:116	laccase immobilization	95:116	laccase immobilization	95:116	Synthesis of novel magnetic cellulose-chitosan composite microspheres and their application in laccase immobilization.
25924363	4	1	with	property	794:801	arg1	magnetization					819:831	saturation magnetization	808:831	saturation magnetization of 30.1 emu g(-1)	808:849	The microshpheres were examined to be with the mean size of 20 μm and good magnetic property with saturation magnetization of 30.1 emu g(-1).
25924363	7	2	theme	reaction	1171:1178	arg1	cycles					1180:1185	12 reaction cycles	1168:1185	12 reaction cycles	1168:1185	Immobilized laccase retained 88.9% activity after 12 reaction cycles.
25924363	1	3	theme	sol-gel	193:199	arg1	method					212:217	sol-gel transition method	193:217	sol-gel transition method using ionic liquids as solvent	193:248	Novel magnetic cellulose-chitosan composite microspheres were prepared by sol-gel transition method using ionic liquids as solvent for dissolution and regeneration.
25924363	6	4	theme	free	955:958	arg1	laccase					960:966	free laccase	955:966	free laccase	955:966	Compared with free laccase, the pH, thermal and operational stabilities of the immobilized laccase were improved and the activity recovery of immobilized laccase reached 80.6%.
25924363	6	4	theme	free	955:958	arg1	pH					973:974	the pH	969:974	the pH	969:974	Compared with free laccase, the pH, thermal and operational stabilities of the immobilized laccase were improved and the activity recovery of immobilized laccase reached 80.6%.
25924363	5	5	from	effect	856:861	arg1	immobilization					892:905	the immobilization	888:905	the immobilization of laccase	888:916	The effect of pH and temperature on the immobilization of laccase was also investigated.
25924363	1	6	theme	transition	201:210	arg1	method					212:217	sol-gel transition method	193:217	sol-gel transition method using ionic liquids as solvent	193:248	Novel magnetic cellulose-chitosan composite microspheres were prepared by sol-gel transition method using ionic liquids as solvent for dissolution and regeneration.
25924363	8	7	theme	composite	1222:1230	arg1	support					1273:1279	a novel support	1265:1279	a novel support for enzyme immobilization	1265:1305	Therefore, the cellulose-chitosan composite microspheres were expected to be a novel support for enzyme immobilization.
25924363	8	7	theme	composite	1222:1230	arg1	microspheres					1232:1243	the cellulose-chitosan composite microspheres	1199:1243	the cellulose-chitosan composite microspheres	1199:1243	Therefore, the cellulose-chitosan composite microspheres were expected to be a novel support for enzyme immobilization.
25924363	0	8	from	Synthesis	0:8	arg1	immobilization					103:116	laccase immobilization	95:116	laccase immobilization	95:116	Synthesis of novel magnetic cellulose-chitosan composite microspheres and their application in laccase immobilization.
25924363	4	9	theme	g	845:845	arg1	magnetization					819:831	saturation magnetization	808:831	saturation magnetization of 30.1 emu g(-1)	808:849	The microshpheres were examined to be with the mean size of 20 μm and good magnetic property with saturation magnetization of 30.1 emu g(-1).
25924363	3	10	theme	nm	649:650	arg1	size					637:640	mean size	632:640	mean size of -10 nm	632:650	Which of their structure, properties and morphology were studied by scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, and vibrating-sample magnetometer showed Fe3O4 nanoparticles with mean size of -10 nm were successfully embedded in the composite microspheres.
25924363	8	11	theme	cellulose-chitosan	1203:1220	arg1	support					1273:1279	a novel support	1265:1279	a novel support for enzyme immobilization	1265:1305	Therefore, the cellulose-chitosan composite microspheres were expected to be a novel support for enzyme immobilization.
25924363	8	11	theme	cellulose-chitosan	1203:1220	arg1	microspheres					1232:1243	the cellulose-chitosan composite microspheres	1199:1243	the cellulose-chitosan composite microspheres	1199:1243	Therefore, the cellulose-chitosan composite microspheres were expected to be a novel support for enzyme immobilization.
25924363	5	12	theme	pH	866:867	arg1	effect					856:861	The effect	852:861	The effect of pH and temperature on the immobilization of laccase	852:916	The effect of pH and temperature on the immobilization of laccase was also investigated.
25924363	3	13	with	nanoparticles	613:625	arg1	size					637:640	mean size	632:640	mean size of -10 nm	632:650	Which of their structure, properties and morphology were studied by scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, and vibrating-sample magnetometer showed Fe3O4 nanoparticles with mean size of -10 nm were successfully embedded in the composite microspheres.
25924363	4	14	theme	emu	841:843	arg1	g					845:845	30.1 emu g	836:845	30.1 emu g(-1)	836:849	The microshpheres were examined to be with the mean size of 20 μm and good magnetic property with saturation magnetization of 30.1 emu g(-1).
25924363	4	14	theme	emu	841:843	arg1	-1					847:848	-1	847:848	-1	847:848	The microshpheres were examined to be with the mean size of 20 μm and good magnetic property with saturation magnetization of 30.1 emu g(-1).
25924363	3	15	from	microspheres	696:707	arg1	embedded					670:677	embedded	670:677	embedded	670:677	Which of their structure, properties and morphology were studied by scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, and vibrating-sample magnetometer showed Fe3O4 nanoparticles with mean size of -10 nm were successfully embedded in the composite microspheres.
25924363	6	16	theme	laccase	1032:1038	arg1	stabilities					1001:1011	thermal and operational stabilities	977:1011	thermal and operational stabilities of the immobilized laccase	977:1038	Compared with free laccase, the pH, thermal and operational stabilities of the immobilized laccase were improved and the activity recovery of immobilized laccase reached 80.6%.
25924363	4	17	theme	μm	773:774	arg1	property					794:801	good magnetic property	780:801	good magnetic property	780:801	The microshpheres were examined to be with the mean size of 20 μm and good magnetic property with saturation magnetization of 30.1 emu g(-1).
25924363	4	17	theme	μm	773:774	arg1	size					762:765	the mean size	753:765	the mean size of 20 μm	753:774	The microshpheres were examined to be with the mean size of 20 μm and good magnetic property with saturation magnetization of 30.1 emu g(-1).
25924363	4	18	theme	magnetic	785:792	arg1	property					794:801	good magnetic property	780:801	good magnetic property	780:801	The microshpheres were examined to be with the mean size of 20 μm and good magnetic property with saturation magnetization of 30.1 emu g(-1).
25924363	7	19	theme	%	1151:1151	arg1	activity					1153:1160	88.9% activity	1147:1160	88.9% activity	1147:1160	Immobilized laccase retained 88.9% activity after 12 reaction cycles.
25924363	5	20	theme	laccase	910:916	arg1	immobilization					892:905	the immobilization	888:905	the immobilization of laccase	888:916	The effect of pH and temperature on the immobilization of laccase was also investigated.
25924363	0	21	theme	composite	47:55	arg1	microspheres					57:68	novel magnetic cellulose-chitosan composite microspheres	13:68	novel magnetic cellulose-chitosan composite microspheres	13:68	Synthesis of novel magnetic cellulose-chitosan composite microspheres and their application in laccase immobilization.
25924363	3	22	theme	mean	632:635	arg1	size					637:640	mean size	632:640	mean size of -10 nm	632:650	Which of their structure, properties and morphology were studied by scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, and vibrating-sample magnetometer showed Fe3O4 nanoparticles with mean size of -10 nm were successfully embedded in the composite microspheres.
25924363	3	23	theme	scanning	442:449	arg1	microscopy					460:469	scanning electron microscopy	442:469	scanning electron microscopy	442:469	Which of their structure, properties and morphology were studied by scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, and vibrating-sample magnetometer showed Fe3O4 nanoparticles with mean size of -10 nm were successfully embedded in the composite microspheres.
25924363	1	24	theme	ionic	225:229	arg1	liquids					231:237	ionic liquids	225:237	ionic liquids	225:237	Novel magnetic cellulose-chitosan composite microspheres were prepared by sol-gel transition method using ionic liquids as solvent for dissolution and regeneration.
25924363	3	25	theme	electron	451:458	arg1	microscopy					460:469	scanning electron microscopy	442:469	scanning electron microscopy	442:469	Which of their structure, properties and morphology were studied by scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, and vibrating-sample magnetometer showed Fe3O4 nanoparticles with mean size of -10 nm were successfully embedded in the composite microspheres.
25924363	6	26	theme	immobilized	1020:1030	arg1	laccase					1032:1038	the immobilized laccase	1016:1038	the immobilized laccase	1016:1038	Compared with free laccase, the pH, thermal and operational stabilities of the immobilized laccase were improved and the activity recovery of immobilized laccase reached 80.6%.
25924363	7	27	theme	88.9	1147:1150	arg1	%					1151:1151	%	1151:1151	%	1151:1151	Immobilized laccase retained 88.9% activity after 12 reaction cycles.
25924363	3	28	dep	showed	600:605	arg1	embedded					670:677	embedded	670:677	embedded	670:677	Which of their structure, properties and morphology were studied by scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, and vibrating-sample magnetometer showed Fe3O4 nanoparticles with mean size of -10 nm were successfully embedded in the composite microspheres.
25924363	3	29	from	embedded	670:677	arg1	microspheres					696:707	the composite microspheres	682:707	the composite microspheres	682:707	Which of their structure, properties and morphology were studied by scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, and vibrating-sample magnetometer showed Fe3O4 nanoparticles with mean size of -10 nm were successfully embedded in the composite microspheres.
25924363	4	30	theme	mean	757:760	arg1	size					762:765	the mean size	753:765	the mean size of 20 μm	753:774	The microshpheres were examined to be with the mean size of 20 μm and good magnetic property with saturation magnetization of 30.1 emu g(-1).
25924363	0	31	theme	magnetic	19:26	arg1	microspheres					57:68	novel magnetic cellulose-chitosan composite microspheres	13:68	novel magnetic cellulose-chitosan composite microspheres	13:68	Synthesis of novel magnetic cellulose-chitosan composite microspheres and their application in laccase immobilization.
25924363	4	32	theme	saturation	808:817	arg1	magnetization					819:831	saturation magnetization	808:831	saturation magnetization of 30.1 emu g(-1)	808:849	The microshpheres were examined to be with the mean size of 20 μm and good magnetic property with saturation magnetization of 30.1 emu g(-1).
25924363	7	33	theme	Immobilized	1118:1128	arg1	laccase					1130:1136	Immobilized laccase	1118:1136	Immobilized laccase	1118:1136	Immobilized laccase retained 88.9% activity after 12 reaction cycles.
25924363	1	34	theme	Novel	119:123	arg1	microspheres					163:174	Novel magnetic cellulose-chitosan composite microspheres	119:174	Novel magnetic cellulose-chitosan composite microspheres	119:174	Novel magnetic cellulose-chitosan composite microspheres were prepared by sol-gel transition method using ionic liquids as solvent for dissolution and regeneration.
25924363	0	35	theme	novel	13:17	arg1	microspheres					57:68	novel magnetic cellulose-chitosan composite microspheres	13:68	novel magnetic cellulose-chitosan composite microspheres	13:68	Synthesis of novel magnetic cellulose-chitosan composite microspheres and their application in laccase immobilization.
25924363	4	36	with	size	762:765	arg1	magnetization					819:831	saturation magnetization	808:831	saturation magnetization of 30.1 emu g(-1)	808:849	The microshpheres were examined to be with the mean size of 20 μm and good magnetic property with saturation magnetization of 30.1 emu g(-1).
25924363	1	37	theme	magnetic	125:132	arg1	microspheres					163:174	Novel magnetic cellulose-chitosan composite microspheres	119:174	Novel magnetic cellulose-chitosan composite microspheres	119:174	Novel magnetic cellulose-chitosan composite microspheres were prepared by sol-gel transition method using ionic liquids as solvent for dissolution and regeneration.
25924363	3	38	theme	X-ray	547:551	arg1	diffraction					553:563	X-ray diffraction	547:563	X-ray diffraction	547:563	Which of their structure, properties and morphology were studied by scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, and vibrating-sample magnetometer showed Fe3O4 nanoparticles with mean size of -10 nm were successfully embedded in the composite microspheres.
25924363	3	39	theme	transmission	472:483	arg1	microscopy					494:503	transmission electron microscopy	472:503	transmission electron microscopy	472:503	Which of their structure, properties and morphology were studied by scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, and vibrating-sample magnetometer showed Fe3O4 nanoparticles with mean size of -10 nm were successfully embedded in the composite microspheres.
25924363	3	40	theme	vibrating-sample	570:585	arg1	magnetometer					587:598	vibrating-sample magnetometer	570:598	vibrating-sample magnetometer	570:598	Which of their structure, properties and morphology were studied by scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, and vibrating-sample magnetometer showed Fe3O4 nanoparticles with mean size of -10 nm were successfully embedded in the composite microspheres.
25924363	6	41	theme	laccase	1095:1101	arg1	recovery					1071:1078	the activity recovery	1058:1078	the activity recovery of immobilized laccase	1058:1101	Compared with free laccase, the pH, thermal and operational stabilities of the immobilized laccase were improved and the activity recovery of immobilized laccase reached 80.6%.
25924363	3	42	theme	infrared	524:531	arg1	spectroscopy					533:544	infrared spectroscopy	524:544	infrared spectroscopy	524:544	Which of their structure, properties and morphology were studied by scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, and vibrating-sample magnetometer showed Fe3O4 nanoparticles with mean size of -10 nm were successfully embedded in the composite microspheres.
25924363	3	43	theme	electron	485:492	arg1	microscopy					494:503	transmission electron microscopy	472:503	transmission electron microscopy	472:503	Which of their structure, properties and morphology were studied by scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, and vibrating-sample magnetometer showed Fe3O4 nanoparticles with mean size of -10 nm were successfully embedded in the composite microspheres.
25924363	6	44	theme	operational	989:999	arg1	stabilities					1001:1011	thermal and operational stabilities	977:1011	thermal and operational stabilities of the immobilized laccase	977:1038	Compared with free laccase, the pH, thermal and operational stabilities of the immobilized laccase were improved and the activity recovery of immobilized laccase reached 80.6%.
25924363	0	45	theme	cellulose-chitosan	28:45	arg1	microspheres					57:68	novel magnetic cellulose-chitosan composite microspheres	13:68	novel magnetic cellulose-chitosan composite microspheres	13:68	Synthesis of novel magnetic cellulose-chitosan composite microspheres and their application in laccase immobilization.
25924363	0	46	from	application	80:90	arg1	immobilization					103:116	laccase immobilization	95:116	laccase immobilization	95:116	Synthesis of novel magnetic cellulose-chitosan composite microspheres and their application in laccase immobilization.
25924363	3	47	theme	composite	686:694	arg1	microspheres					696:707	the composite microspheres	682:707	the composite microspheres	682:707	Which of their structure, properties and morphology were studied by scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, and vibrating-sample magnetometer showed Fe3O4 nanoparticles with mean size of -10 nm were successfully embedded in the composite microspheres.
25924363	6	48	theme	immobilized	1083:1093	arg1	laccase					1095:1101	immobilized laccase	1083:1101	immobilized laccase	1083:1101	Compared with free laccase, the pH, thermal and operational stabilities of the immobilized laccase were improved and the activity recovery of immobilized laccase reached 80.6%.
25924363	8	49	theme	enzyme	1285:1290	arg1	immobilization					1292:1305	enzyme immobilization	1285:1305	enzyme immobilization	1285:1305	Therefore, the cellulose-chitosan composite microspheres were expected to be a novel support for enzyme immobilization.
25924363	8	50	theme	novel	1267:1271	arg1	support					1273:1279	a novel support	1265:1279	a novel support for enzyme immobilization	1265:1305	Therefore, the cellulose-chitosan composite microspheres were expected to be a novel support for enzyme immobilization.
25924363	8	50	theme	novel	1267:1271	arg1	microspheres					1232:1243	the cellulose-chitosan composite microspheres	1199:1243	the cellulose-chitosan composite microspheres	1199:1243	Therefore, the cellulose-chitosan composite microspheres were expected to be a novel support for enzyme immobilization.
25924363	3	51	dep	Fourier	506:512	arg1	transform					514:522	transform	514:522	transform infrared spectroscopy	514:544	Which of their structure, properties and morphology were studied by scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, and vibrating-sample magnetometer showed Fe3O4 nanoparticles with mean size of -10 nm were successfully embedded in the composite microspheres.
25924363	1	52	theme	cellulose-chitosan	134:151	arg1	microspheres					163:174	Novel magnetic cellulose-chitosan composite microspheres	119:174	Novel magnetic cellulose-chitosan composite microspheres	119:174	Novel magnetic cellulose-chitosan composite microspheres were prepared by sol-gel transition method using ionic liquids as solvent for dissolution and regeneration.
25924363	0	53	theme	microspheres	57:68	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of novel magnetic cellulose-chitosan composite microspheres	0:68	Synthesis of novel magnetic cellulose-chitosan composite microspheres and their application in laccase immobilization.
25924363	0	53	theme	microspheres	57:68	arg1	application					80:90	their application	74:90	their application in laccase immobilization	74:116	Synthesis of novel magnetic cellulose-chitosan composite microspheres and their application in laccase immobilization.
25924363	6	54	theme	thermal	977:983	arg1	stabilities					1001:1011	thermal and operational stabilities	977:1011	thermal and operational stabilities of the immobilized laccase	977:1038	Compared with free laccase, the pH, thermal and operational stabilities of the immobilized laccase were improved and the activity recovery of immobilized laccase reached 80.6%.
25924363	1	55	theme	composite	153:161	arg1	microspheres					163:174	Novel magnetic cellulose-chitosan composite microspheres	119:174	Novel magnetic cellulose-chitosan composite microspheres	119:174	Novel magnetic cellulose-chitosan composite microspheres were prepared by sol-gel transition method using ionic liquids as solvent for dissolution and regeneration.
25924363	2	56	theme	composite	302:310	arg1	microspheres					312:323	the composite microspheres	298:323	the composite microspheres activated by glutaradehyde	298:350	Subsequently, the composite microspheres activated by glutaradehyde to immobilize enzyme.
25924363	3	57	theme	Fe3O4	607:611	arg1	nanoparticles					613:625	Fe3O4 nanoparticles	607:625	Fe3O4 nanoparticles with mean size of -10 nm	607:650	Which of their structure, properties and morphology were studied by scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, and vibrating-sample magnetometer showed Fe3O4 nanoparticles with mean size of -10 nm were successfully embedded in the composite microspheres.
25924363	6	58	theme	activity	1062:1069	arg1	recovery					1071:1078	the activity recovery	1058:1078	the activity recovery of immobilized laccase	1058:1101	Compared with free laccase, the pH, thermal and operational stabilities of the immobilized laccase were improved and the activity recovery of immobilized laccase reached 80.6%.
25924363	5	59	theme	temperature	873:883	arg1	effect					856:861	The effect	852:861	The effect of pH and temperature on the immobilization of laccase	852:916	The effect of pH and temperature on the immobilization of laccase was also investigated.
25924363	4	60	theme	good	780:783	arg1	property					794:801	good magnetic property	780:801	good magnetic property	780:801	The microshpheres were examined to be with the mean size of 20 μm and good magnetic property with saturation magnetization of 30.1 emu g(-1).
26794743	0	0	theme	durable	94:100	arg1	resistance					105:114	durable UV resistance	94:114	durable UV resistance	94:114	Cotton fabric with plasma pretreatment and ZnO/Carboxymethyl chitosan composite finishing for durable UV resistance and antibacterial property.
26794743	1	1	theme	Scanning	338:345	arg1	SEM					368:370	SEM	368:370	SEM	368:370	ZnO/carboxymethyl chitosan (ZnO/CMCS) composite was prepared and confirmed by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Ultraviolet-visible (UV-vis) spectroscopy, Scanning electron microscope (SEM), Transmission electron microscope (TEM).
26794743	1	1	theme	Scanning	338:345	arg1	spectroscopy					249:260	infrared spectroscopy	240:260	infrared spectroscopy (FTIR)	240:267	ZnO/carboxymethyl chitosan (ZnO/CMCS) composite was prepared and confirmed by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Ultraviolet-visible (UV-vis) spectroscopy, Scanning electron microscope (SEM), Transmission electron microscope (TEM).
26794743	1	1	theme	Scanning	338:345	arg1	microscope					356:365	Scanning electron microscope	338:365	Scanning electron microscope (SEM)	338:371	ZnO/carboxymethyl chitosan (ZnO/CMCS) composite was prepared and confirmed by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Ultraviolet-visible (UV-vis) spectroscopy, Scanning electron microscope (SEM), Transmission electron microscope (TEM).
26794743	6	2	theme	increasing	1102:1111	arg1	concentration					1113:1125	the increasing concentration	1098:1125	the increasing concentration of ZnO/CMCS composite	1098:1147	UV resistance and antibacterial activity of the finished cotton fabric were greatly improved, which increased with the increasing concentration of ZnO/CMCS composite.
26794743	7	3	theme	ZnO/CMCS	1246:1253	arg1	composite					1255:1263	ZnO/CMCS composite	1246:1263	ZnO/CMCS composite	1246:1263	TG analysis indicated that the combined finishing of cotton fabric with plasma pretreatment and ZnO/CMCS composite could improve its thermal property.
26794743	1	4	theme	electron	347:354	arg1	SEM					368:370	SEM	368:370	SEM	368:370	ZnO/carboxymethyl chitosan (ZnO/CMCS) composite was prepared and confirmed by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Ultraviolet-visible (UV-vis) spectroscopy, Scanning electron microscope (SEM), Transmission electron microscope (TEM).
26794743	1	4	theme	electron	347:354	arg1	spectroscopy					249:260	infrared spectroscopy	240:260	infrared spectroscopy (FTIR)	240:267	ZnO/carboxymethyl chitosan (ZnO/CMCS) composite was prepared and confirmed by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Ultraviolet-visible (UV-vis) spectroscopy, Scanning electron microscope (SEM), Transmission electron microscope (TEM).
26794743	1	4	theme	electron	347:354	arg1	microscope					356:365	Scanning electron microscope	338:365	Scanning electron microscope (SEM)	338:371	ZnO/carboxymethyl chitosan (ZnO/CMCS) composite was prepared and confirmed by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Ultraviolet-visible (UV-vis) spectroscopy, Scanning electron microscope (SEM), Transmission electron microscope (TEM).
26794743	7	5	theme	TG	1150:1151	arg1	analysis					1153:1160	TG analysis	1150:1160	TG analysis	1150:1160	TG analysis indicated that the combined finishing of cotton fabric with plasma pretreatment and ZnO/CMCS composite could improve its thermal property.
26794743	1	6	theme	X-ray	270:274	arg1	XRD					289:291	XRD	289:291	XRD	289:291	ZnO/carboxymethyl chitosan (ZnO/CMCS) composite was prepared and confirmed by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Ultraviolet-visible (UV-vis) spectroscopy, Scanning electron microscope (SEM), Transmission electron microscope (TEM).
26794743	1	6	theme	X-ray	270:274	arg1	diffraction					276:286	X-ray diffraction	270:286	X-ray diffraction (XRD)	270:292	ZnO/carboxymethyl chitosan (ZnO/CMCS) composite was prepared and confirmed by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Ultraviolet-visible (UV-vis) spectroscopy, Scanning electron microscope (SEM), Transmission electron microscope (TEM).
26794743	1	6	theme	X-ray	270:274	arg1	spectroscopy					249:260	infrared spectroscopy	240:260	infrared spectroscopy (FTIR)	240:267	ZnO/carboxymethyl chitosan (ZnO/CMCS) composite was prepared and confirmed by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Ultraviolet-visible (UV-vis) spectroscopy, Scanning electron microscope (SEM), Transmission electron microscope (TEM).
26794743	7	7	theme	plasma	1222:1227	arg1	pretreatment					1229:1240	plasma pretreatment	1222:1240	plasma pretreatment	1222:1240	TG analysis indicated that the combined finishing of cotton fabric with plasma pretreatment and ZnO/CMCS composite could improve its thermal property.
26794743	3	8	theme	finishing	655:663	arg1	ZnO/CMCS					636:643	the ZnO/CMCS composite finishing	632:663	the ZnO/CMCS composite finishing	632:663	Cotton fabric was pretreated by cold oxygen plasma and the ZnO/CMCS composite finishing was carried out by pad-dry-cure.
26794743	4	9	theme	antibacterial	759:771	arg1	activity					773:780	antibacterial activity	759:780	antibacterial activity	759:780	Cotton fabric was characterized by SEM, FTIR, UV resistance, antibacterial activity and Thermogravimetry (TG).
26794743	0	10	theme	UV	102:103	arg1	resistance					105:114	durable UV resistance	94:114	durable UV resistance	94:114	Cotton fabric with plasma pretreatment and ZnO/Carboxymethyl chitosan composite finishing for durable UV resistance and antibacterial property.
26794743	8	11	theme	UV	1376:1377	arg1	resistance					1379:1388	UV resistance	1376:1388	UV resistance	1376:1388	The finished cotton fabric exhibited an excellent laundering durability in UV resistance and antibacterial activity.
26794743	6	12	theme	ZnO/CMCS	1130:1137	arg1	composite					1139:1147	ZnO/CMCS composite	1130:1147	ZnO/CMCS composite	1130:1147	UV resistance and antibacterial activity of the finished cotton fabric were greatly improved, which increased with the increasing concentration of ZnO/CMCS composite.
26794743	2	13	theme	UV	517:518	arg1	resistance					520:529	durable UV resistance	509:529	durable UV resistance	509:529	The combination of plasma pretreatment and ZnO/CMCS composite finishing was applied to provide durable UV resistance and antibacterial activity for cotton fabric.
26794743	7	14	theme	thermal	1283:1289	arg1	property					1291:1298	its thermal property	1279:1298	its thermal property	1279:1298	TG analysis indicated that the combined finishing of cotton fabric with plasma pretreatment and ZnO/CMCS composite could improve its thermal property.
26794743	2	15	theme	durable	509:515	arg1	resistance					520:529	durable UV resistance	509:529	durable UV resistance	509:529	The combination of plasma pretreatment and ZnO/CMCS composite finishing was applied to provide durable UV resistance and antibacterial activity for cotton fabric.
26794743	7	16	with	composite	1255:1263	arg1	pretreatment					1229:1240	plasma pretreatment	1222:1240	plasma pretreatment	1222:1240	TG analysis indicated that the combined finishing of cotton fabric with plasma pretreatment and ZnO/CMCS composite could improve its thermal property.
26794743	5	17	theme	ZnO/CMCS	937:944	arg1	composite					946:954	ZnO/CMCS composite	937:954	ZnO/CMCS composite owing to plasma treatment	937:980	SEM and FTIR analysis demonstrated the presence of ZnO/CMCS composite on cotton fabric and the increasing loading efficiency of ZnO/CMCS composite owing to plasma treatment.
26794743	8	18	theme	finished	1305:1312	arg1	fabric					1321:1326	The finished cotton fabric	1301:1326	The finished cotton fabric	1301:1326	The finished cotton fabric exhibited an excellent laundering durability in UV resistance and antibacterial activity.
26794743	0	19	theme	antibacterial	120:132	arg1	property					134:141	antibacterial property	120:141	antibacterial property	120:141	Cotton fabric with plasma pretreatment and ZnO/Carboxymethyl chitosan composite finishing for durable UV resistance and antibacterial property.
26794743	2	20	theme	pretreatment	440:451	arg1	combination					418:428	The combination	414:428	The combination of plasma pretreatment	414:451	The combination of plasma pretreatment and ZnO/CMCS composite finishing was applied to provide durable UV resistance and antibacterial activity for cotton fabric.
26794743	2	20	theme	pretreatment	440:451	arg1	pretreatment					440:451	plasma pretreatment	433:451	plasma pretreatment	433:451	The combination of plasma pretreatment and ZnO/CMCS composite finishing was applied to provide durable UV resistance and antibacterial activity for cotton fabric.
26794743	2	20	theme	pretreatment	440:451	arg1	composite					466:474	ZnO/CMCS composite finishing	457:484	ZnO/CMCS composite finishing	457:484	The combination of plasma pretreatment and ZnO/CMCS composite finishing was applied to provide durable UV resistance and antibacterial activity for cotton fabric.
26794743	3	21	theme	Cotton	577:582	arg1	fabric					584:589	Cotton fabric	577:589	Cotton fabric	577:589	Cotton fabric was pretreated by cold oxygen plasma and the ZnO/CMCS composite finishing was carried out by pad-dry-cure.
26794743	5	22	theme	composite	869:877	arg1	presence					848:855	the presence	844:855	the presence of ZnO/CMCS composite on cotton fabric and the increasing loading efficiency of ZnO/CMCS composite owing to plasma treatment	844:980	SEM and FTIR analysis demonstrated the presence of ZnO/CMCS composite on cotton fabric and the increasing loading efficiency of ZnO/CMCS composite owing to plasma treatment.
26794743	2	23	theme	plasma	433:438	arg1	pretreatment					440:451	plasma pretreatment	433:451	plasma pretreatment	433:451	The combination of plasma pretreatment and ZnO/CMCS composite finishing was applied to provide durable UV resistance and antibacterial activity for cotton fabric.
26794743	6	24	theme	UV	983:984	arg1	resistance					986:995	UV resistance	983:995	UV resistance	983:995	UV resistance and antibacterial activity of the finished cotton fabric were greatly improved, which increased with the increasing concentration of ZnO/CMCS composite.
26794743	8	25	from	durability	1362:1371	arg1	resistance					1379:1388	UV resistance	1376:1388	UV resistance	1376:1388	The finished cotton fabric exhibited an excellent laundering durability in UV resistance and antibacterial activity.
26794743	8	25	from	durability	1362:1371	arg1	activity					1408:1415	antibacterial activity	1394:1415	antibacterial activity	1394:1415	The finished cotton fabric exhibited an excellent laundering durability in UV resistance and antibacterial activity.
26794743	5	26	theme	composite	946:954	arg1	efficiency					923:932	the increasing loading efficiency	900:932	the increasing loading efficiency of ZnO/CMCS composite owing to plasma treatment	900:980	SEM and FTIR analysis demonstrated the presence of ZnO/CMCS composite on cotton fabric and the increasing loading efficiency of ZnO/CMCS composite owing to plasma treatment.
26794743	5	26	theme	composite	946:954	arg1	fabric					889:894	cotton fabric	882:894	cotton fabric	882:894	SEM and FTIR analysis demonstrated the presence of ZnO/CMCS composite on cotton fabric and the increasing loading efficiency of ZnO/CMCS composite owing to plasma treatment.
26794743	0	27	theme	plasma	19:24	arg1	pretreatment					26:37	plasma pretreatment	19:37	plasma pretreatment	19:37	Cotton fabric with plasma pretreatment and ZnO/Carboxymethyl chitosan composite finishing for durable UV resistance and antibacterial property.
26794743	5	28	theme	SEM	809:811	arg1	analysis					822:829	SEM and FTIR analysis	809:829	analysis	822:829	SEM and FTIR analysis demonstrated the presence of ZnO/CMCS composite on cotton fabric and the increasing loading efficiency of ZnO/CMCS composite owing to plasma treatment.
26794743	2	29	theme	cotton	562:567	arg1	fabric					569:574	cotton fabric	562:574	cotton fabric	562:574	The combination of plasma pretreatment and ZnO/CMCS composite finishing was applied to provide durable UV resistance and antibacterial activity for cotton fabric.
26794743	5	30	theme	FTIR	817:820	arg1	analysis					822:829	SEM and FTIR analysis	809:829	analysis	822:829	SEM and FTIR analysis demonstrated the presence of ZnO/CMCS composite on cotton fabric and the increasing loading efficiency of ZnO/CMCS composite owing to plasma treatment.
26794743	1	31	theme	Transmission	374:385	arg1	microscope					396:405	Transmission electron microscope	374:405	Transmission electron microscope (TEM)	374:411	ZnO/carboxymethyl chitosan (ZnO/CMCS) composite was prepared and confirmed by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Ultraviolet-visible (UV-vis) spectroscopy, Scanning electron microscope (SEM), Transmission electron microscope (TEM).
26794743	1	31	theme	Transmission	374:385	arg1	spectroscopy					249:260	infrared spectroscopy	240:260	infrared spectroscopy (FTIR)	240:267	ZnO/carboxymethyl chitosan (ZnO/CMCS) composite was prepared and confirmed by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Ultraviolet-visible (UV-vis) spectroscopy, Scanning electron microscope (SEM), Transmission electron microscope (TEM).
26794743	1	31	theme	Transmission	374:385	arg1	TEM					408:410	TEM	408:410	TEM	408:410	ZnO/carboxymethyl chitosan (ZnO/CMCS) composite was prepared and confirmed by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Ultraviolet-visible (UV-vis) spectroscopy, Scanning electron microscope (SEM), Transmission electron microscope (TEM).
26794743	4	32	theme	UV	744:745	arg1	resistance					747:756	UV resistance	744:756	UV resistance	744:756	Cotton fabric was characterized by SEM, FTIR, UV resistance, antibacterial activity and Thermogravimetry (TG).
26794743	5	33	theme	cotton	882:887	arg1	fabric					889:894	cotton fabric	882:894	cotton fabric	882:894	SEM and FTIR analysis demonstrated the presence of ZnO/CMCS composite on cotton fabric and the increasing loading efficiency of ZnO/CMCS composite owing to plasma treatment.
26794743	2	34	theme	finishing	476:484	arg1	pretreatment					440:451	plasma pretreatment	433:451	plasma pretreatment	433:451	The combination of plasma pretreatment and ZnO/CMCS composite finishing was applied to provide durable UV resistance and antibacterial activity for cotton fabric.
26794743	2	34	theme	finishing	476:484	arg1	composite					466:474	ZnO/CMCS composite finishing	457:484	ZnO/CMCS composite finishing	457:484	The combination of plasma pretreatment and ZnO/CMCS composite finishing was applied to provide durable UV resistance and antibacterial activity for cotton fabric.
26794743	1	35	theme	Ultraviolet-visible	295:313	arg1	spectroscopy					249:260	infrared spectroscopy	240:260	infrared spectroscopy (FTIR)	240:267	ZnO/carboxymethyl chitosan (ZnO/CMCS) composite was prepared and confirmed by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Ultraviolet-visible (UV-vis) spectroscopy, Scanning electron microscope (SEM), Transmission electron microscope (TEM).
26794743	1	35	theme	Ultraviolet-visible	295:313	arg1	spectroscopy					324:335	Ultraviolet-visible (UV-vis) spectroscopy	295:335	Ultraviolet-visible (UV-vis) spectroscopy	295:335	ZnO/carboxymethyl chitosan (ZnO/CMCS) composite was prepared and confirmed by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Ultraviolet-visible (UV-vis) spectroscopy, Scanning electron microscope (SEM), Transmission electron microscope (TEM).
26794743	1	36	theme	electron	387:394	arg1	microscope					396:405	Transmission electron microscope	374:405	Transmission electron microscope (TEM)	374:411	ZnO/carboxymethyl chitosan (ZnO/CMCS) composite was prepared and confirmed by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Ultraviolet-visible (UV-vis) spectroscopy, Scanning electron microscope (SEM), Transmission electron microscope (TEM).
26794743	1	36	theme	electron	387:394	arg1	spectroscopy					249:260	infrared spectroscopy	240:260	infrared spectroscopy (FTIR)	240:267	ZnO/carboxymethyl chitosan (ZnO/CMCS) composite was prepared and confirmed by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Ultraviolet-visible (UV-vis) spectroscopy, Scanning electron microscope (SEM), Transmission electron microscope (TEM).
26794743	1	36	theme	electron	387:394	arg1	TEM					408:410	TEM	408:410	TEM	408:410	ZnO/carboxymethyl chitosan (ZnO/CMCS) composite was prepared and confirmed by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Ultraviolet-visible (UV-vis) spectroscopy, Scanning electron microscope (SEM), Transmission electron microscope (TEM).
26794743	7	37	with	fabric	1210:1215	arg1	pretreatment					1229:1240	plasma pretreatment	1222:1240	plasma pretreatment	1222:1240	TG analysis indicated that the combined finishing of cotton fabric with plasma pretreatment and ZnO/CMCS composite could improve its thermal property.
26794743	8	38	theme	excellent	1341:1349	arg1	durability					1362:1371	an excellent laundering durability	1338:1371	an excellent laundering durability in UV resistance and antibacterial activity	1338:1415	The finished cotton fabric exhibited an excellent laundering durability in UV resistance and antibacterial activity.
26794743	8	39	theme	cotton	1314:1319	arg1	fabric					1321:1326	The finished cotton fabric	1301:1326	The finished cotton fabric	1301:1326	The finished cotton fabric exhibited an excellent laundering durability in UV resistance and antibacterial activity.
26794743	2	40	theme	antibacterial	535:547	arg1	activity					549:556	antibacterial activity	535:556	antibacterial activity	535:556	The combination of plasma pretreatment and ZnO/CMCS composite finishing was applied to provide durable UV resistance and antibacterial activity for cotton fabric.
26794743	6	41	theme	antibacterial	1001:1013	arg1	activity					1015:1022	antibacterial activity	1001:1022	antibacterial activity	1001:1022	UV resistance and antibacterial activity of the finished cotton fabric were greatly improved, which increased with the increasing concentration of ZnO/CMCS composite.
26794743	3	42	theme	cold	609:612	arg1	plasma					621:626	cold oxygen plasma	609:626	cold oxygen plasma	609:626	Cotton fabric was pretreated by cold oxygen plasma and the ZnO/CMCS composite finishing was carried out by pad-dry-cure.
26794743	1	43	theme	infrared	240:247	arg1	microscope					396:405	Transmission electron microscope	374:405	Transmission electron microscope (TEM)	374:411	ZnO/carboxymethyl chitosan (ZnO/CMCS) composite was prepared and confirmed by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Ultraviolet-visible (UV-vis) spectroscopy, Scanning electron microscope (SEM), Transmission electron microscope (TEM).
26794743	1	43	theme	infrared	240:247	arg1	spectroscopy					249:260	infrared spectroscopy	240:260	infrared spectroscopy (FTIR)	240:267	ZnO/carboxymethyl chitosan (ZnO/CMCS) composite was prepared and confirmed by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Ultraviolet-visible (UV-vis) spectroscopy, Scanning electron microscope (SEM), Transmission electron microscope (TEM).
26794743	1	43	theme	infrared	240:247	arg1	spectroscopy					324:335	Ultraviolet-visible (UV-vis) spectroscopy	295:335	Ultraviolet-visible (UV-vis) spectroscopy	295:335	ZnO/carboxymethyl chitosan (ZnO/CMCS) composite was prepared and confirmed by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Ultraviolet-visible (UV-vis) spectroscopy, Scanning electron microscope (SEM), Transmission electron microscope (TEM).
26794743	1	43	theme	infrared	240:247	arg1	FTIR					263:266	FTIR	263:266	FTIR	263:266	ZnO/carboxymethyl chitosan (ZnO/CMCS) composite was prepared and confirmed by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Ultraviolet-visible (UV-vis) spectroscopy, Scanning electron microscope (SEM), Transmission electron microscope (TEM).
26794743	1	43	theme	infrared	240:247	arg1	microscope					356:365	Scanning electron microscope	338:365	Scanning electron microscope (SEM)	338:371	ZnO/carboxymethyl chitosan (ZnO/CMCS) composite was prepared and confirmed by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Ultraviolet-visible (UV-vis) spectroscopy, Scanning electron microscope (SEM), Transmission electron microscope (TEM).
26794743	1	43	theme	infrared	240:247	arg1	diffraction					276:286	X-ray diffraction	270:286	X-ray diffraction (XRD)	270:292	ZnO/carboxymethyl chitosan (ZnO/CMCS) composite was prepared and confirmed by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Ultraviolet-visible (UV-vis) spectroscopy, Scanning electron microscope (SEM), Transmission electron microscope (TEM).
26794743	2	44	theme	ZnO/CMCS	457:464	arg1	pretreatment					440:451	plasma pretreatment	433:451	plasma pretreatment	433:451	The combination of plasma pretreatment and ZnO/CMCS composite finishing was applied to provide durable UV resistance and antibacterial activity for cotton fabric.
26794743	2	44	theme	ZnO/CMCS	457:464	arg1	composite					466:474	ZnO/CMCS composite finishing	457:484	ZnO/CMCS composite finishing	457:484	The combination of plasma pretreatment and ZnO/CMCS composite finishing was applied to provide durable UV resistance and antibacterial activity for cotton fabric.
26794743	1	45	theme	UV-vis	316:321	arg1	spectroscopy					249:260	infrared spectroscopy	240:260	infrared spectroscopy (FTIR)	240:267	ZnO/carboxymethyl chitosan (ZnO/CMCS) composite was prepared and confirmed by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Ultraviolet-visible (UV-vis) spectroscopy, Scanning electron microscope (SEM), Transmission electron microscope (TEM).
26794743	1	45	theme	UV-vis	316:321	arg1	spectroscopy					324:335	Ultraviolet-visible (UV-vis) spectroscopy	295:335	Ultraviolet-visible (UV-vis) spectroscopy	295:335	ZnO/carboxymethyl chitosan (ZnO/CMCS) composite was prepared and confirmed by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Ultraviolet-visible (UV-vis) spectroscopy, Scanning electron microscope (SEM), Transmission electron microscope (TEM).
26794743	3	46	theme	composite	645:653	arg1	ZnO/CMCS					636:643	the ZnO/CMCS composite finishing	632:663	the ZnO/CMCS composite finishing	632:663	Cotton fabric was pretreated by cold oxygen plasma and the ZnO/CMCS composite finishing was carried out by pad-dry-cure.
26794743	5	47	from	presence	848:855	arg1	efficiency					923:932	the increasing loading efficiency	900:932	the increasing loading efficiency of ZnO/CMCS composite owing to plasma treatment	900:980	SEM and FTIR analysis demonstrated the presence of ZnO/CMCS composite on cotton fabric and the increasing loading efficiency of ZnO/CMCS composite owing to plasma treatment.
26794743	5	47	from	presence	848:855	arg1	fabric					889:894	cotton fabric	882:894	cotton fabric	882:894	SEM and FTIR analysis demonstrated the presence of ZnO/CMCS composite on cotton fabric and the increasing loading efficiency of ZnO/CMCS composite owing to plasma treatment.
26794743	5	48	theme	increasing	904:913	arg1	efficiency					923:932	the increasing loading efficiency	900:932	the increasing loading efficiency of ZnO/CMCS composite owing to plasma treatment	900:980	SEM and FTIR analysis demonstrated the presence of ZnO/CMCS composite on cotton fabric and the increasing loading efficiency of ZnO/CMCS composite owing to plasma treatment.
26794743	0	49	theme	ZnO/Carboxymethyl	43:59	arg1	chitosan					61:68	ZnO/Carboxymethyl chitosan composite	43:78	ZnO/Carboxymethyl chitosan composite	43:78	Cotton fabric with plasma pretreatment and ZnO/Carboxymethyl chitosan composite finishing for durable UV resistance and antibacterial property.
26794743	0	50	with	fabric	7:12	arg1	pretreatment					26:37	plasma pretreatment	19:37	plasma pretreatment	19:37	Cotton fabric with plasma pretreatment and ZnO/Carboxymethyl chitosan composite finishing for durable UV resistance and antibacterial property.
26794743	0	50	with	fabric	7:12	arg1	chitosan					61:68	ZnO/Carboxymethyl chitosan composite	43:78	ZnO/Carboxymethyl chitosan composite	43:78	Cotton fabric with plasma pretreatment and ZnO/Carboxymethyl chitosan composite finishing for durable UV resistance and antibacterial property.
26794743	5	51	theme	loading	915:921	arg1	efficiency					923:932	the increasing loading efficiency	900:932	the increasing loading efficiency of ZnO/CMCS composite owing to plasma treatment	900:980	SEM and FTIR analysis demonstrated the presence of ZnO/CMCS composite on cotton fabric and the increasing loading efficiency of ZnO/CMCS composite owing to plasma treatment.
26794743	6	52	theme	finished	1031:1038	arg1	fabric					1047:1052	the finished cotton fabric	1027:1052	the finished cotton fabric	1027:1052	UV resistance and antibacterial activity of the finished cotton fabric were greatly improved, which increased with the increasing concentration of ZnO/CMCS composite.
26794743	1	53	theme	ZnO/carboxymethyl	144:160	arg1	ZnO/CMCS					172:179	ZnO/CMCS	172:179	ZnO/CMCS	172:179	ZnO/carboxymethyl chitosan (ZnO/CMCS) composite was prepared and confirmed by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Ultraviolet-visible (UV-vis) spectroscopy, Scanning electron microscope (SEM), Transmission electron microscope (TEM).
26794743	1	53	theme	ZnO/carboxymethyl	144:160	arg1	chitosan					162:169	ZnO/carboxymethyl chitosan	144:169	ZnO/carboxymethyl chitosan (ZnO/CMCS) composite	144:190	ZnO/carboxymethyl chitosan (ZnO/CMCS) composite was prepared and confirmed by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Ultraviolet-visible (UV-vis) spectroscopy, Scanning electron microscope (SEM), Transmission electron microscope (TEM).
26794743	6	54	theme	cotton	1040:1045	arg1	fabric					1047:1052	the finished cotton fabric	1027:1052	the finished cotton fabric	1027:1052	UV resistance and antibacterial activity of the finished cotton fabric were greatly improved, which increased with the increasing concentration of ZnO/CMCS composite.
26794743	8	55	theme	antibacterial	1394:1406	arg1	activity					1408:1415	antibacterial activity	1394:1415	antibacterial activity	1394:1415	The finished cotton fabric exhibited an excellent laundering durability in UV resistance and antibacterial activity.
26794743	5	56	attach	presence	848:855	arg2	composite					869:877	ZnO/CMCS composite	860:877	ZnO/CMCS composite	860:877	SEM and FTIR analysis demonstrated the presence of ZnO/CMCS composite on cotton fabric and the increasing loading efficiency of ZnO/CMCS composite owing to plasma treatment.
26794743	5	56	attach	presence	848:855	arg1	efficiency					923:932	the increasing loading efficiency	900:932	the increasing loading efficiency of ZnO/CMCS composite owing to plasma treatment	900:980	SEM and FTIR analysis demonstrated the presence of ZnO/CMCS composite on cotton fabric and the increasing loading efficiency of ZnO/CMCS composite owing to plasma treatment.
26794743	5	56	attach	presence	848:855	arg1	fabric					889:894	cotton fabric	882:894	cotton fabric	882:894	SEM and FTIR analysis demonstrated the presence of ZnO/CMCS composite on cotton fabric and the increasing loading efficiency of ZnO/CMCS composite owing to plasma treatment.
26794743	5	57	theme	plasma	965:970	arg1	treatment					972:980	plasma treatment	965:980	plasma treatment	965:980	SEM and FTIR analysis demonstrated the presence of ZnO/CMCS composite on cotton fabric and the increasing loading efficiency of ZnO/CMCS composite owing to plasma treatment.
26794743	1	58	theme	chitosan	162:169	arg1	composite					182:190	ZnO/carboxymethyl chitosan (ZnO/CMCS) composite	144:190	ZnO/carboxymethyl chitosan (ZnO/CMCS) composite	144:190	ZnO/carboxymethyl chitosan (ZnO/CMCS) composite was prepared and confirmed by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Ultraviolet-visible (UV-vis) spectroscopy, Scanning electron microscope (SEM), Transmission electron microscope (TEM).
26794743	6	59	theme	composite	1139:1147	arg1	concentration					1113:1125	the increasing concentration	1098:1125	the increasing concentration of ZnO/CMCS composite	1098:1147	UV resistance and antibacterial activity of the finished cotton fabric were greatly improved, which increased with the increasing concentration of ZnO/CMCS composite.
26794743	7	60	theme	composite	1255:1263	arg1	finishing					1190:1198	finishing	1190:1198	finishing	1190:1198	TG analysis indicated that the combined finishing of cotton fabric with plasma pretreatment and ZnO/CMCS composite could improve its thermal property.
26794743	6	61	theme	fabric	1047:1052	arg1	resistance					986:995	UV resistance	983:995	UV resistance	983:995	UV resistance and antibacterial activity of the finished cotton fabric were greatly improved, which increased with the increasing concentration of ZnO/CMCS composite.
26794743	6	61	theme	fabric	1047:1052	arg1	activity					1015:1022	antibacterial activity	1001:1022	antibacterial activity	1001:1022	UV resistance and antibacterial activity of the finished cotton fabric were greatly improved, which increased with the increasing concentration of ZnO/CMCS composite.
26794743	3	62	theme	oxygen	614:619	arg1	plasma					621:626	cold oxygen plasma	609:626	cold oxygen plasma	609:626	Cotton fabric was pretreated by cold oxygen plasma and the ZnO/CMCS composite finishing was carried out by pad-dry-cure.
26794743	4	63	theme	Cotton	698:703	arg1	fabric					705:710	Cotton fabric	698:710	Cotton fabric	698:710	Cotton fabric was characterized by SEM, FTIR, UV resistance, antibacterial activity and Thermogravimetry (TG).
26794743	5	64	theme	ZnO/CMCS	860:867	arg1	composite					869:877	ZnO/CMCS composite	860:877	ZnO/CMCS composite	860:877	SEM and FTIR analysis demonstrated the presence of ZnO/CMCS composite on cotton fabric and the increasing loading efficiency of ZnO/CMCS composite owing to plasma treatment.
26794743	0	65	theme	composite	70:78	arg1	chitosan					61:68	ZnO/Carboxymethyl chitosan composite	43:78	ZnO/Carboxymethyl chitosan composite	43:78	Cotton fabric with plasma pretreatment and ZnO/Carboxymethyl chitosan composite finishing for durable UV resistance and antibacterial property.
26794743	7	66	theme	fabric	1210:1215	arg1	finishing					1190:1198	finishing	1190:1198	finishing	1190:1198	TG analysis indicated that the combined finishing of cotton fabric with plasma pretreatment and ZnO/CMCS composite could improve its thermal property.
26794743	1	67	dep	Fourier	222:228	arg1	transform					230:238	transform	230:238	transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Ultraviolet-visible (UV-vis) spectroscopy, Scanning electron microscope (SEM), Transmission electron microscope (TEM)	230:411	ZnO/carboxymethyl chitosan (ZnO/CMCS) composite was prepared and confirmed by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Ultraviolet-visible (UV-vis) spectroscopy, Scanning electron microscope (SEM), Transmission electron microscope (TEM).
26794743	8	68	theme	laundering	1351:1360	arg1	durability					1362:1371	an excellent laundering durability	1338:1371	an excellent laundering durability in UV resistance and antibacterial activity	1338:1415	The finished cotton fabric exhibited an excellent laundering durability in UV resistance and antibacterial activity.
26794743	7	69	theme	cotton	1203:1208	arg1	fabric					1210:1215	cotton fabric	1203:1215	cotton fabric with plasma pretreatment	1203:1240	TG analysis indicated that the combined finishing of cotton fabric with plasma pretreatment and ZnO/CMCS composite could improve its thermal property.
27599938	8	0	theme	blood	1593:1597	arg1	assay					1623:1627	a peripheral blood neutrophil opsonisation assay	1580:1627	a peripheral blood neutrophil opsonisation assay	1580:1627	Moreover, whilst antibodies raised in fish against the wild type cross-reacted in whole cell and cps ELISA, they did not cross-opsonise the mutant in a peripheral blood neutrophil opsonisation assay, consistent with reported vaccine escape.
27599938	6	1	theme	CpsG	1281:1284	arg1	activity					1269:1276	epimerase activity	1259:1276	epimerase activity of CpsG	1259:1284	Deletion of cpsG resulted in changes to colony morphology and cell buoyant density, and also significantly decreased galactose content relative to glucose in the capsular polysaccharide as determined by GC-MS, consistent with epimerase activity of CpsG.
27599938	7	2	theme	fish	1418:1421	arg1	blood					1423:1427	whole fish blood	1412:1427	whole fish blood	1412:1427	There was also a metabolic penalty of cpsG knockout revealed by slower growth in complex media, and reduced proliferation in whole fish blood.
27599938	8	3	theme	opsonisation	1610:1621	arg1	assay					1623:1627	a peripheral blood neutrophil opsonisation assay	1580:1627	a peripheral blood neutrophil opsonisation assay	1580:1627	Moreover, whilst antibodies raised in fish against the wild type cross-reacted in whole cell and cps ELISA, they did not cross-opsonise the mutant in a peripheral blood neutrophil opsonisation assay, consistent with reported vaccine escape.
27599938	6	4	theme	relative	1168:1175	arg1	content					1160:1166	galactose content	1150:1166	galactose content relative to glucose in the capsular polysaccharide	1150:1217	Deletion of cpsG resulted in changes to colony morphology and cell buoyant density, and also significantly decreased galactose content relative to glucose in the capsular polysaccharide as determined by GC-MS, consistent with epimerase activity of CpsG.
27599938	1	5	theme	marine	180:185	arg1	fish					202:205	marine and freshwater fish	180:205	fish	202:205	Streptococcus iniae causes septicaemia and meningitis in marine and freshwater fish wherever they are farmed in warm-temperate and tropical regions.
27599938	6	6	theme	galactose	1150:1158	arg1	content					1160:1166	galactose content	1150:1166	galactose content relative to glucose in the capsular polysaccharide	1150:1217	Deletion of cpsG resulted in changes to colony morphology and cell buoyant density, and also significantly decreased galactose content relative to glucose in the capsular polysaccharide as determined by GC-MS, consistent with epimerase activity of CpsG.
27599938	2	7	dep	Although	272:279	arg1	specific					290:297	specific	290:297	specific	290:297	Although serotype specific, vaccination with bacterins (killed bacterial cultures) is largely successful and vaccine failure occurs only occasionally through emergence of new capsular serotypes.
27599938	3	8	theme	polysaccharide	598:611	arg1	operon					619:624	the 20-gene capsular polysaccharide (cps) operon	577:624	the 20-gene capsular polysaccharide (cps) operon	577:624	Previously we showed that mutations in vaccine escapes are restricted to a limited repertoire of genes within the 20-gene capsular polysaccharide (cps) operon.
27599938	1	9	theme	freshwater	191:200	arg1	fish					202:205	marine and freshwater fish	180:205	fish	202:205	Streptococcus iniae causes septicaemia and meningitis in marine and freshwater fish wherever they are farmed in warm-temperate and tropical regions.
27599938	4	10	theme	UDP-galactose	644:656	arg1	4-epimerase					658:668	a putative UDP-galactose 4-epimerase	633:668	a putative UDP-galactose 4-epimerase	633:668	cpsG, a putative UDP-galactose 4-epimerase, has three sequence types based on the insertion or deletion of the three amino acids leucine, serine and lysine in the substrate binding site of the protein.
27599938	4	10	theme	UDP-galactose	644:656	arg1	cpsG					627:630	cpsG	627:630	cpsG	627:630	cpsG, a putative UDP-galactose 4-epimerase, has three sequence types based on the insertion or deletion of the three amino acids leucine, serine and lysine in the substrate binding site of the protein.
27599938	3	11	theme	20-gene	581:587	arg1	cps					614:616	cps	614:616	cps	614:616	Previously we showed that mutations in vaccine escapes are restricted to a limited repertoire of genes within the 20-gene capsular polysaccharide (cps) operon.
27599938	3	11	theme	20-gene	581:587	arg1	polysaccharide					598:611	20-gene capsular polysaccharide	581:611	the 20-gene capsular polysaccharide (cps) operon	577:624	Previously we showed that mutations in vaccine escapes are restricted to a limited repertoire of genes within the 20-gene capsular polysaccharide (cps) operon.
27599938	0	12	from	cause	75:79	arg1	fish					117:120	vaccinated fish	106:120	vaccinated fish	106:120	Streptococcus iniae cpsG alters capsular carbohydrate composition and is a cause of serotype switching in vaccinated fish.
27599938	7	13	theme	complex	1368:1374	arg1	media					1376:1380	complex media	1368:1380	complex media	1368:1380	There was also a metabolic penalty of cpsG knockout revealed by slower growth in complex media, and reduced proliferation in whole fish blood.
27599938	1	14	from	regions	263:269	arg1	farmed					225:230	farmed	225:230	farmed	225:230	Streptococcus iniae causes septicaemia and meningitis in marine and freshwater fish wherever they are farmed in warm-temperate and tropical regions.
27599938	4	15	theme	leucine	756:762	arg1	deletion					722:729	deletion	722:729	deletion	722:729	cpsG, a putative UDP-galactose 4-epimerase, has three sequence types based on the insertion or deletion of the three amino acids leucine, serine and lysine in the substrate binding site of the protein.
27599938	4	15	theme	leucine	756:762	arg1	insertion					709:717	insertion	709:717	insertion	709:717	cpsG, a putative UDP-galactose 4-epimerase, has three sequence types based on the insertion or deletion of the three amino acids leucine, serine and lysine in the substrate binding site of the protein.
27599938	5	16	theme	capsular	909:916	arg1	composition					918:928	capsular composition	909:928	capsular composition	909:928	To elucidate the role of cpsG in capsular polysaccharide (CPS) biosynthesis and capsular composition, we first prepared isogenic knockout and complemented mutants of cpsG by allelic exchange mutagenesis.
27599938	7	17	theme	knockout	1330:1337	arg1	cpsG					1325:1328	cpsG knockout	1325:1337	cpsG knockout revealed by slower growth in complex media	1325:1380	There was also a metabolic penalty of cpsG knockout revealed by slower growth in complex media, and reduced proliferation in whole fish blood.
27599938	9	18	theme	poor	1775:1778	arg1	cross-opsonisation					1780:1797	poor cross-opsonisation	1775:1797	poor cross-opsonisation that explains some of the historic vaccination failure on fish farms in Australia	1775:1879	We have shown here that mutation in cpsG results in altered CPS composition and this in turn results in poor cross-opsonisation that explains some of the historic vaccination failure on fish farms in Australia.
27599938	5	19	from	role	846:849	arg1	biosynthesis					892:903	capsular polysaccharide (CPS) biosynthesis	862:903	capsular polysaccharide (CPS) biosynthesis	862:903	To elucidate the role of cpsG in capsular polysaccharide (CPS) biosynthesis and capsular composition, we first prepared isogenic knockout and complemented mutants of cpsG by allelic exchange mutagenesis.
27599938	5	19	from	role	846:849	arg1	composition					918:928	capsular composition	909:928	capsular composition	909:928	To elucidate the role of cpsG in capsular polysaccharide (CPS) biosynthesis and capsular composition, we first prepared isogenic knockout and complemented mutants of cpsG by allelic exchange mutagenesis.
27599938	5	20	theme	polysaccharide	871:884	arg1	biosynthesis					892:903	capsular polysaccharide (CPS) biosynthesis	862:903	capsular polysaccharide (CPS) biosynthesis	862:903	To elucidate the role of cpsG in capsular polysaccharide (CPS) biosynthesis and capsular composition, we first prepared isogenic knockout and complemented mutants of cpsG by allelic exchange mutagenesis.
27599938	9	21	from	mutation	1695:1702	arg1	results					1712:1718	cpsG results	1707:1718	cpsG results	1707:1718	We have shown here that mutation in cpsG results in altered CPS composition and this in turn results in poor cross-opsonisation that explains some of the historic vaccination failure on fish farms in Australia.
27599938	9	21	from	mutation	1695:1702	arg1	this					1751:1754	this	1751:1754	this	1751:1754	We have shown here that mutation in cpsG results in altered CPS composition and this in turn results in poor cross-opsonisation that explains some of the historic vaccination failure on fish farms in Australia.
27599938	9	21	from	mutation	1695:1702	arg1	composition					1735:1745	altered CPS composition	1723:1745	altered CPS composition	1723:1745	We have shown here that mutation in cpsG results in altered CPS composition and this in turn results in poor cross-opsonisation that explains some of the historic vaccination failure on fish farms in Australia.
27599938	9	22	theme	altered	1723:1729	arg1	composition					1735:1745	altered CPS composition	1723:1745	altered CPS composition	1723:1745	We have shown here that mutation in cpsG results in altered CPS composition and this in turn results in poor cross-opsonisation that explains some of the historic vaccination failure on fish farms in Australia.
27599938	4	23	from	lysine	776:781	arg1	site					808:811	the substrate binding site	786:811	the substrate binding site of the protein	786:826	cpsG, a putative UDP-galactose 4-epimerase, has three sequence types based on the insertion or deletion of the three amino acids leucine, serine and lysine in the substrate binding site of the protein.
27599938	4	23	from	lysine	776:781	arg1	protein					820:826	the protein	816:826	the protein	816:826	cpsG, a putative UDP-galactose 4-epimerase, has three sequence types based on the insertion or deletion of the three amino acids leucine, serine and lysine in the substrate binding site of the protein.
27599938	7	24	theme	slower	1351:1356	arg1	growth					1358:1363	slower growth	1351:1363	slower growth in complex media	1351:1380	There was also a metabolic penalty of cpsG knockout revealed by slower growth in complex media, and reduced proliferation in whole fish blood.
27599938	0	25	theme	serotype	84:91	arg1	switching					93:101	serotype switching	84:101	serotype switching in vaccinated fish	84:120	Streptococcus iniae cpsG alters capsular carbohydrate composition and is a cause of serotype switching in vaccinated fish.
27599938	4	26	from	serine	765:770	arg1	site					808:811	the substrate binding site	786:811	the substrate binding site of the protein	786:826	cpsG, a putative UDP-galactose 4-epimerase, has three sequence types based on the insertion or deletion of the three amino acids leucine, serine and lysine in the substrate binding site of the protein.
27599938	4	26	from	serine	765:770	arg1	protein					820:826	the protein	816:826	the protein	816:826	cpsG, a putative UDP-galactose 4-epimerase, has three sequence types based on the insertion or deletion of the three amino acids leucine, serine and lysine in the substrate binding site of the protein.
27599938	3	27	dep	vaccine	506:512	arg1	escapes					514:520	escapes	514:520	escapes	514:520	Previously we showed that mutations in vaccine escapes are restricted to a limited repertoire of genes within the 20-gene capsular polysaccharide (cps) operon.
27599938	7	28	theme	cpsG	1325:1328	arg1	penalty					1314:1320	a metabolic penalty	1302:1320	a metabolic penalty of cpsG knockout revealed by slower growth in complex media	1302:1380	There was also a metabolic penalty of cpsG knockout revealed by slower growth in complex media, and reduced proliferation in whole fish blood.
27599938	7	28	theme	cpsG	1325:1328	arg1	proliferation					1395:1407	proliferation	1395:1407	proliferation	1395:1407	There was also a metabolic penalty of cpsG knockout revealed by slower growth in complex media, and reduced proliferation in whole fish blood.
27599938	8	29	theme	whole	1512:1516	arg1	cell					1518:1521	whole cell	1512:1521	whole cell	1512:1521	Moreover, whilst antibodies raised in fish against the wild type cross-reacted in whole cell and cps ELISA, they did not cross-opsonise the mutant in a peripheral blood neutrophil opsonisation assay, consistent with reported vaccine escape.
27599938	4	30	theme	amino	744:748	arg1	acids					750:754	three amino acids	738:754	the three amino acids leucine	734:762	cpsG, a putative UDP-galactose 4-epimerase, has three sequence types based on the insertion or deletion of the three amino acids leucine, serine and lysine in the substrate binding site of the protein.
27599938	0	31	theme	Streptococcus	0:12	arg1	cpsG					20:23	Streptococcus iniae cpsG	0:23	Streptococcus iniae cpsG	0:23	Streptococcus iniae cpsG alters capsular carbohydrate composition and is a cause of serotype switching in vaccinated fish.
27599938	7	32	from	growth	1358:1363	arg1	media					1376:1380	complex media	1368:1380	complex media	1368:1380	There was also a metabolic penalty of cpsG knockout revealed by slower growth in complex media, and reduced proliferation in whole fish blood.
27599938	2	33	theme	serotypes	456:464	arg1	emergence					430:438	emergence	430:438	emergence of new capsular serotypes	430:464	Although serotype specific, vaccination with bacterins (killed bacterial cultures) is largely successful and vaccine failure occurs only occasionally through emergence of new capsular serotypes.
27599938	1	34	dep	causes	143:148	arg1	farmed					225:230	farmed	225:230	farmed	225:230	Streptococcus iniae causes septicaemia and meningitis in marine and freshwater fish wherever they are farmed in warm-temperate and tropical regions.
27599938	2	35	theme	new	443:445	arg1	serotypes					456:464	new capsular serotypes	443:464	new capsular serotypes	443:464	Although serotype specific, vaccination with bacterins (killed bacterial cultures) is largely successful and vaccine failure occurs only occasionally through emergence of new capsular serotypes.
27599938	0	36	theme	capsular	32:39	arg1	composition					54:64	capsular carbohydrate composition	32:64	capsular carbohydrate composition	32:64	Streptococcus iniae cpsG alters capsular carbohydrate composition and is a cause of serotype switching in vaccinated fish.
27599938	5	37	theme	allelic	1003:1009	arg1	mutagenesis					1020:1030	allelic exchange mutagenesis	1003:1030	allelic exchange mutagenesis	1003:1030	To elucidate the role of cpsG in capsular polysaccharide (CPS) biosynthesis and capsular composition, we first prepared isogenic knockout and complemented mutants of cpsG by allelic exchange mutagenesis.
27599938	4	38	theme	protein	820:826	arg1	site					808:811	the substrate binding site	786:811	the substrate binding site of the protein	786:826	cpsG, a putative UDP-galactose 4-epimerase, has three sequence types based on the insertion or deletion of the three amino acids leucine, serine and lysine in the substrate binding site of the protein.
27599938	4	38	theme	protein	820:826	arg1	protein					820:826	the protein	816:826	the protein	816:826	cpsG, a putative UDP-galactose 4-epimerase, has three sequence types based on the insertion or deletion of the three amino acids leucine, serine and lysine in the substrate binding site of the protein.
27599938	3	39	from	mutations	493:501	arg1	vaccine					506:512	vaccine escapes	506:520	vaccine escapes	506:520	Previously we showed that mutations in vaccine escapes are restricted to a limited repertoire of genes within the 20-gene capsular polysaccharide (cps) operon.
27599938	6	40	theme	buoyant	1100:1106	arg1	density					1108:1114	cell buoyant density	1095:1114	cell buoyant density	1095:1114	Deletion of cpsG resulted in changes to colony morphology and cell buoyant density, and also significantly decreased galactose content relative to glucose in the capsular polysaccharide as determined by GC-MS, consistent with epimerase activity of CpsG.
27599938	9	41	theme	historic	1825:1832	arg1	failure					1846:1852	the historic vaccination failure	1821:1852	the historic vaccination failure	1821:1852	We have shown here that mutation in cpsG results in altered CPS composition and this in turn results in poor cross-opsonisation that explains some of the historic vaccination failure on fish farms in Australia.
27599938	4	42	theme	binding	800:806	arg1	site					808:811	the substrate binding site	786:811	the substrate binding site of the protein	786:826	cpsG, a putative UDP-galactose 4-epimerase, has three sequence types based on the insertion or deletion of the three amino acids leucine, serine and lysine in the substrate binding site of the protein.
27599938	4	42	theme	binding	800:806	arg1	protein					820:826	the protein	816:826	the protein	816:826	cpsG, a putative UDP-galactose 4-epimerase, has three sequence types based on the insertion or deletion of the three amino acids leucine, serine and lysine in the substrate binding site of the protein.
27599938	2	43	theme	vaccine	381:387	arg1	failure					389:395	vaccine failure	381:395	vaccine failure	381:395	Although serotype specific, vaccination with bacterins (killed bacterial cultures) is largely successful and vaccine failure occurs only occasionally through emergence of new capsular serotypes.
27599938	1	44	theme	warm-temperate	235:248	arg1	regions					263:269	warm-temperate and tropical regions	235:269	warm-temperate and tropical regions	235:269	Streptococcus iniae causes septicaemia and meningitis in marine and freshwater fish wherever they are farmed in warm-temperate and tropical regions.
27599938	5	45	theme	cpsG	995:998	arg1	mutants					984:990	mutants	984:990	mutants of cpsG	984:998	To elucidate the role of cpsG in capsular polysaccharide (CPS) biosynthesis and capsular composition, we first prepared isogenic knockout and complemented mutants of cpsG by allelic exchange mutagenesis.
27599938	0	46	from	fish	117:120	arg1	cause					75:79	a cause	73:79	a cause of serotype switching in vaccinated fish	73:120	Streptococcus iniae cpsG alters capsular carbohydrate composition and is a cause of serotype switching in vaccinated fish.
27599938	8	47	theme	reported	1646:1653	arg1	escape					1663:1668	reported vaccine escape	1646:1668	reported vaccine escape	1646:1668	Moreover, whilst antibodies raised in fish against the wild type cross-reacted in whole cell and cps ELISA, they did not cross-opsonise the mutant in a peripheral blood neutrophil opsonisation assay, consistent with reported vaccine escape.
27599938	1	48	theme	tropical	254:261	arg1	regions					263:269	warm-temperate and tropical regions	235:269	warm-temperate and tropical regions	235:269	Streptococcus iniae causes septicaemia and meningitis in marine and freshwater fish wherever they are farmed in warm-temperate and tropical regions.
27599938	6	49	theme	colony	1073:1078	arg1	morphology					1080:1089	colony morphology	1073:1089	colony morphology	1073:1089	Deletion of cpsG resulted in changes to colony morphology and cell buoyant density, and also significantly decreased galactose content relative to glucose in the capsular polysaccharide as determined by GC-MS, consistent with epimerase activity of CpsG.
27599938	9	50	theme	fish	1857:1860	arg1	farms					1862:1866	fish farms	1857:1866	fish farms in Australia	1857:1879	We have shown here that mutation in cpsG results in altered CPS composition and this in turn results in poor cross-opsonisation that explains some of the historic vaccination failure on fish farms in Australia.
27599938	8	51	theme	peripheral	1582:1591	arg1	assay					1623:1627	a peripheral blood neutrophil opsonisation assay	1580:1627	a peripheral blood neutrophil opsonisation assay	1580:1627	Moreover, whilst antibodies raised in fish against the wild type cross-reacted in whole cell and cps ELISA, they did not cross-opsonise the mutant in a peripheral blood neutrophil opsonisation assay, consistent with reported vaccine escape.
27599938	3	52	theme	genes	564:568	arg1	genes					564:568	genes	564:568	genes within the 20-gene capsular polysaccharide (cps) operon	564:624	Previously we showed that mutations in vaccine escapes are restricted to a limited repertoire of genes within the 20-gene capsular polysaccharide (cps) operon.
27599938	3	52	theme	genes	564:568	arg1	repertoire					550:559	a limited repertoire	540:559	a limited repertoire of genes within the 20-gene capsular polysaccharide (cps) operon	540:624	Previously we showed that mutations in vaccine escapes are restricted to a limited repertoire of genes within the 20-gene capsular polysaccharide (cps) operon.
27599938	3	53	theme	capsular	589:596	arg1	cps					614:616	cps	614:616	cps	614:616	Previously we showed that mutations in vaccine escapes are restricted to a limited repertoire of genes within the 20-gene capsular polysaccharide (cps) operon.
27599938	3	53	theme	capsular	589:596	arg1	polysaccharide					598:611	20-gene capsular polysaccharide	581:611	the 20-gene capsular polysaccharide (cps) operon	577:624	Previously we showed that mutations in vaccine escapes are restricted to a limited repertoire of genes within the 20-gene capsular polysaccharide (cps) operon.
27599938	9	54	from	farms	1862:1866	arg1	Australia					1871:1879	Australia	1871:1879	Australia	1871:1879	We have shown here that mutation in cpsG results in altered CPS composition and this in turn results in poor cross-opsonisation that explains some of the historic vaccination failure on fish farms in Australia.
27599938	8	55	theme	neutrophil	1599:1608	arg1	assay					1623:1627	a peripheral blood neutrophil opsonisation assay	1580:1627	a peripheral blood neutrophil opsonisation assay	1580:1627	Moreover, whilst antibodies raised in fish against the wild type cross-reacted in whole cell and cps ELISA, they did not cross-opsonise the mutant in a peripheral blood neutrophil opsonisation assay, consistent with reported vaccine escape.
27599938	6	56	theme	epimerase	1259:1267	arg1	activity					1269:1276	epimerase activity	1259:1276	epimerase activity of CpsG	1259:1284	Deletion of cpsG resulted in changes to colony morphology and cell buoyant density, and also significantly decreased galactose content relative to glucose in the capsular polysaccharide as determined by GC-MS, consistent with epimerase activity of CpsG.
27599938	7	57	theme	whole	1412:1416	arg1	blood					1423:1427	whole fish blood	1412:1427	whole fish blood	1412:1427	There was also a metabolic penalty of cpsG knockout revealed by slower growth in complex media, and reduced proliferation in whole fish blood.
27599938	2	58	theme	killed	328:333	arg1	bacterins					317:325	bacterins	317:325	bacterins (killed bacterial cultures)	317:353	Although serotype specific, vaccination with bacterins (killed bacterial cultures) is largely successful and vaccine failure occurs only occasionally through emergence of new capsular serotypes.
27599938	2	58	theme	killed	328:333	arg1	cultures					345:352	killed bacterial cultures	328:352	killed bacterial cultures	328:352	Although serotype specific, vaccination with bacterins (killed bacterial cultures) is largely successful and vaccine failure occurs only occasionally through emergence of new capsular serotypes.
27599938	5	59	theme	cpsG	854:857	arg1	role					846:849	the role	842:849	the role of cpsG in capsular polysaccharide (CPS) biosynthesis and capsular composition	842:928	To elucidate the role of cpsG in capsular polysaccharide (CPS) biosynthesis and capsular composition, we first prepared isogenic knockout and complemented mutants of cpsG by allelic exchange mutagenesis.
27599938	4	60	theme	sequence	681:688	arg1	types					690:694	three sequence types	675:694	three sequence types	675:694	cpsG, a putative UDP-galactose 4-epimerase, has three sequence types based on the insertion or deletion of the three amino acids leucine, serine and lysine in the substrate binding site of the protein.
27599938	4	61	theme	putative	635:642	arg1	4-epimerase					658:668	a putative UDP-galactose 4-epimerase	633:668	a putative UDP-galactose 4-epimerase	633:668	cpsG, a putative UDP-galactose 4-epimerase, has three sequence types based on the insertion or deletion of the three amino acids leucine, serine and lysine in the substrate binding site of the protein.
27599938	4	61	theme	putative	635:642	arg1	cpsG					627:630	cpsG	627:630	cpsG	627:630	cpsG, a putative UDP-galactose 4-epimerase, has three sequence types based on the insertion or deletion of the three amino acids leucine, serine and lysine in the substrate binding site of the protein.
27599938	8	62	theme	wild	1485:1488	arg1	type					1490:1493	the wild type	1481:1493	the wild type	1481:1493	Moreover, whilst antibodies raised in fish against the wild type cross-reacted in whole cell and cps ELISA, they did not cross-opsonise the mutant in a peripheral blood neutrophil opsonisation assay, consistent with reported vaccine escape.
27599938	1	63	from	farmed	225:230	arg1	regions					263:269	warm-temperate and tropical regions	235:269	warm-temperate and tropical regions	235:269	Streptococcus iniae causes septicaemia and meningitis in marine and freshwater fish wherever they are farmed in warm-temperate and tropical regions.
27599938	9	64	theme	cpsG	1707:1710	arg1	results					1712:1718	cpsG results	1707:1718	cpsG results	1707:1718	We have shown here that mutation in cpsG results in altered CPS composition and this in turn results in poor cross-opsonisation that explains some of the historic vaccination failure on fish farms in Australia.
27599938	0	65	from	switching	93:101	arg1	fish					117:120	vaccinated fish	106:120	vaccinated fish	106:120	Streptococcus iniae cpsG alters capsular carbohydrate composition and is a cause of serotype switching in vaccinated fish.
27599938	6	66	theme	capsular	1195:1202	arg1	polysaccharide					1204:1217	the capsular polysaccharide	1191:1217	the capsular polysaccharide	1191:1217	Deletion of cpsG resulted in changes to colony morphology and cell buoyant density, and also significantly decreased galactose content relative to glucose in the capsular polysaccharide as determined by GC-MS, consistent with epimerase activity of CpsG.
27599938	5	67	theme	capsular	862:869	arg1	CPS					887:889	CPS	887:889	CPS	887:889	To elucidate the role of cpsG in capsular polysaccharide (CPS) biosynthesis and capsular composition, we first prepared isogenic knockout and complemented mutants of cpsG by allelic exchange mutagenesis.
27599938	5	67	theme	capsular	862:869	arg1	polysaccharide					871:884	capsular polysaccharide	862:884	capsular polysaccharide (CPS) biosynthesis	862:903	To elucidate the role of cpsG in capsular polysaccharide (CPS) biosynthesis and capsular composition, we first prepared isogenic knockout and complemented mutants of cpsG by allelic exchange mutagenesis.
27599938	2	68	with	vaccination	300:310	arg1	bacterins					317:325	bacterins	317:325	bacterins (killed bacterial cultures)	317:353	Although serotype specific, vaccination with bacterins (killed bacterial cultures) is largely successful and vaccine failure occurs only occasionally through emergence of new capsular serotypes.
27599938	2	68	with	vaccination	300:310	arg1	cultures					345:352	killed bacterial cultures	328:352	killed bacterial cultures	328:352	Although serotype specific, vaccination with bacterins (killed bacterial cultures) is largely successful and vaccine failure occurs only occasionally through emergence of new capsular serotypes.
27599938	9	69	theme	CPS	1731:1733	arg1	composition					1735:1745	altered CPS composition	1723:1745	altered CPS composition	1723:1745	We have shown here that mutation in cpsG results in altered CPS composition and this in turn results in poor cross-opsonisation that explains some of the historic vaccination failure on fish farms in Australia.
27599938	0	70	theme	switching	93:101	arg1	cause					75:79	a cause	73:79	a cause of serotype switching in vaccinated fish	73:120	Streptococcus iniae cpsG alters capsular carbohydrate composition and is a cause of serotype switching in vaccinated fish.
27599938	4	71	dep	insertion	709:717	arg1	the					705:707	the	705:707	the	705:707	cpsG, a putative UDP-galactose 4-epimerase, has three sequence types based on the insertion or deletion of the three amino acids leucine, serine and lysine in the substrate binding site of the protein.
27599938	0	72	theme	vaccinated	106:115	arg1	fish					117:120	vaccinated fish	106:120	vaccinated fish	106:120	Streptococcus iniae cpsG alters capsular carbohydrate composition and is a cause of serotype switching in vaccinated fish.
27599938	6	73	from	glucose	1180:1186	arg1	polysaccharide					1204:1217	the capsular polysaccharide	1191:1217	the capsular polysaccharide	1191:1217	Deletion of cpsG resulted in changes to colony morphology and cell buoyant density, and also significantly decreased galactose content relative to glucose in the capsular polysaccharide as determined by GC-MS, consistent with epimerase activity of CpsG.
27599938	7	74	theme	metabolic	1304:1312	arg1	penalty					1314:1320	a metabolic penalty	1302:1320	a metabolic penalty of cpsG knockout revealed by slower growth in complex media	1302:1380	There was also a metabolic penalty of cpsG knockout revealed by slower growth in complex media, and reduced proliferation in whole fish blood.
27599938	7	74	theme	metabolic	1304:1312	arg1	proliferation					1395:1407	proliferation	1395:1407	proliferation	1395:1407	There was also a metabolic penalty of cpsG knockout revealed by slower growth in complex media, and reduced proliferation in whole fish blood.
27599938	8	75	theme	cps	1527:1529	arg1	ELISA					1531:1535	cps ELISA	1527:1535	cps ELISA	1527:1535	Moreover, whilst antibodies raised in fish against the wild type cross-reacted in whole cell and cps ELISA, they did not cross-opsonise the mutant in a peripheral blood neutrophil opsonisation assay, consistent with reported vaccine escape.
27599938	6	76	dep	resulted	1050:1057	arg1	decreased					1140:1148	decreased	1140:1148	decreased galactose content relative to glucose in the capsular polysaccharide as determined by GC-MS	1140:1240	Deletion of cpsG resulted in changes to colony morphology and cell buoyant density, and also significantly decreased galactose content relative to glucose in the capsular polysaccharide as determined by GC-MS, consistent with epimerase activity of CpsG.
27599938	6	77	theme	cpsG	1045:1048	arg1	Deletion					1033:1040	Deletion	1033:1040	Deletion of cpsG	1033:1048	Deletion of cpsG resulted in changes to colony morphology and cell buoyant density, and also significantly decreased galactose content relative to glucose in the capsular polysaccharide as determined by GC-MS, consistent with epimerase activity of CpsG.
27599938	2	78	theme	capsular	447:454	arg1	serotypes					456:464	new capsular serotypes	443:464	new capsular serotypes	443:464	Although serotype specific, vaccination with bacterins (killed bacterial cultures) is largely successful and vaccine failure occurs only occasionally through emergence of new capsular serotypes.
27599938	8	79	with	consistent	1630:1639	arg1	escape					1663:1668	reported vaccine escape	1646:1668	reported vaccine escape	1646:1668	Moreover, whilst antibodies raised in fish against the wild type cross-reacted in whole cell and cps ELISA, they did not cross-opsonise the mutant in a peripheral blood neutrophil opsonisation assay, consistent with reported vaccine escape.
27599938	4	80	theme	acids	750:754	arg1	serine					765:770	serine	765:770	serine	765:770	cpsG, a putative UDP-galactose 4-epimerase, has three sequence types based on the insertion or deletion of the three amino acids leucine, serine and lysine in the substrate binding site of the protein.
27599938	4	80	theme	acids	750:754	arg1	leucine					756:762	the three amino acids leucine	734:762	the three amino acids leucine	734:762	cpsG, a putative UDP-galactose 4-epimerase, has three sequence types based on the insertion or deletion of the three amino acids leucine, serine and lysine in the substrate binding site of the protein.
27599938	4	80	theme	acids	750:754	arg1	lysine					776:781	lysine	776:781	lysine	776:781	cpsG, a putative UDP-galactose 4-epimerase, has three sequence types based on the insertion or deletion of the three amino acids leucine, serine and lysine in the substrate binding site of the protein.
27599938	0	81	theme	carbohydrate	41:52	arg1	composition					54:64	capsular carbohydrate composition	32:64	capsular carbohydrate composition	32:64	Streptococcus iniae cpsG alters capsular carbohydrate composition and is a cause of serotype switching in vaccinated fish.
27599938	5	82	theme	exchange	1011:1018	arg1	mutagenesis					1020:1030	allelic exchange mutagenesis	1003:1030	allelic exchange mutagenesis	1003:1030	To elucidate the role of cpsG in capsular polysaccharide (CPS) biosynthesis and capsular composition, we first prepared isogenic knockout and complemented mutants of cpsG by allelic exchange mutagenesis.
27599938	0	83	dep	Streptococcus	0:12	arg1	iniae					14:18	iniae	14:18	iniae	14:18	Streptococcus iniae cpsG alters capsular carbohydrate composition and is a cause of serotype switching in vaccinated fish.
27599938	4	84	contain	has	671:673	arg2	types					690:694	three sequence types	675:694	three sequence types	675:694	cpsG, a putative UDP-galactose 4-epimerase, has three sequence types based on the insertion or deletion of the three amino acids leucine, serine and lysine in the substrate binding site of the protein.
27599938	4	84	contain	has	671:673	arg1	cpsG					627:630	cpsG	627:630	cpsG	627:630	cpsG, a putative UDP-galactose 4-epimerase, has three sequence types based on the insertion or deletion of the three amino acids leucine, serine and lysine in the substrate binding site of the protein.
27599938	4	84	contain	has	671:673	arg1	4-epimerase					658:668	a putative UDP-galactose 4-epimerase	633:668	a putative UDP-galactose 4-epimerase	633:668	cpsG, a putative UDP-galactose 4-epimerase, has three sequence types based on the insertion or deletion of the three amino acids leucine, serine and lysine in the substrate binding site of the protein.
27599938	2	85	theme	bacterial	335:343	arg1	bacterins					317:325	bacterins	317:325	bacterins (killed bacterial cultures)	317:353	Although serotype specific, vaccination with bacterins (killed bacterial cultures) is largely successful and vaccine failure occurs only occasionally through emergence of new capsular serotypes.
27599938	2	85	theme	bacterial	335:343	arg1	cultures					345:352	killed bacterial cultures	328:352	killed bacterial cultures	328:352	Although serotype specific, vaccination with bacterins (killed bacterial cultures) is largely successful and vaccine failure occurs only occasionally through emergence of new capsular serotypes.
27599938	6	86	with	consistent	1243:1252	arg1	activity					1269:1276	epimerase activity	1259:1276	epimerase activity of CpsG	1259:1284	Deletion of cpsG resulted in changes to colony morphology and cell buoyant density, and also significantly decreased galactose content relative to glucose in the capsular polysaccharide as determined by GC-MS, consistent with epimerase activity of CpsG.
27599938	6	87	theme	cell	1095:1098	arg1	density					1108:1114	cell buoyant density	1095:1114	cell buoyant density	1095:1114	Deletion of cpsG resulted in changes to colony morphology and cell buoyant density, and also significantly decreased galactose content relative to glucose in the capsular polysaccharide as determined by GC-MS, consistent with epimerase activity of CpsG.
27599938	9	88	theme	vaccination	1834:1844	arg1	failure					1846:1852	the historic vaccination failure	1821:1852	the historic vaccination failure	1821:1852	We have shown here that mutation in cpsG results in altered CPS composition and this in turn results in poor cross-opsonisation that explains some of the historic vaccination failure on fish farms in Australia.
27599938	4	89	theme	substrate	790:798	arg1	site					808:811	the substrate binding site	786:811	the substrate binding site of the protein	786:826	cpsG, a putative UDP-galactose 4-epimerase, has three sequence types based on the insertion or deletion of the three amino acids leucine, serine and lysine in the substrate binding site of the protein.
27599938	4	89	theme	substrate	790:798	arg1	protein					820:826	the protein	816:826	the protein	816:826	cpsG, a putative UDP-galactose 4-epimerase, has three sequence types based on the insertion or deletion of the three amino acids leucine, serine and lysine in the substrate binding site of the protein.
27599938	3	90	theme	limited	542:548	arg1	genes					564:568	genes	564:568	genes within the 20-gene capsular polysaccharide (cps) operon	564:624	Previously we showed that mutations in vaccine escapes are restricted to a limited repertoire of genes within the 20-gene capsular polysaccharide (cps) operon.
27599938	3	90	theme	limited	542:548	arg1	repertoire					550:559	a limited repertoire	540:559	a limited repertoire of genes within the 20-gene capsular polysaccharide (cps) operon	540:624	Previously we showed that mutations in vaccine escapes are restricted to a limited repertoire of genes within the 20-gene capsular polysaccharide (cps) operon.
27599938	8	91	theme	vaccine	1655:1661	arg1	escape					1663:1668	reported vaccine escape	1646:1668	reported vaccine escape	1646:1668	Moreover, whilst antibodies raised in fish against the wild type cross-reacted in whole cell and cps ELISA, they did not cross-opsonise the mutant in a peripheral blood neutrophil opsonisation assay, consistent with reported vaccine escape.
28602986	11	0	theme	relaxation	1419:1428	arg1	process					1430:1436	a completed singular relaxation process	1398:1436	a completed singular relaxation process	1398:1436	The CNF-Si also exhibited a completed singular relaxation process, while the CNF and the CNF-P processes were uncompleted.
28602986	9	1	theme	highest	1237:1243	arg1	strength					1253:1260	the highest tensile strength	1233:1260	the highest tensile strength	1233:1260	The CNF-P displayed the highest tensile strength.
28602986	7	2	theme	permittivity	959:970	arg1	parts					950:954	The real and imaginary parts	927:954	The real and imaginary parts of permittivity and electric modulus	927:991	The real and imaginary parts of permittivity and electric modulus were evaluated using an impedance analyzer.
28602986	0	3	theme	electrical	63:72	arg1	properties					74:83	chemical, thermo-mechanical and electrical properties	31:83	chemical, thermo-mechanical and electrical properties of Celluclast-treated nanofibrillated cellulose composites	31:142	Influence of PVA and silica on chemical, thermo-mechanical and electrical properties of Celluclast-treated nanofibrillated cellulose composites.
28602986	6	4	theme	universal	891:899	arg1	machine					909:915	a universal testing machine	889:915	a universal testing machine	889:915	The Young's and storage moduli of the nanocomposites were determined via a universal testing machine and DTMA.
28602986	3	5	theme	nanofibrillated	569:583	arg1	CNF					596:598	CNF	596:598	CNF	596:598	Three nanocomposite films were fabricated via the casting process: nanofibrillated cellulose (CNF), nanocellulose-PVA (CNF-P) and nanocellulose-silica (CNF-Si).
28602986	3	5	theme	nanofibrillated	569:583	arg1	cellulose					585:593	nanofibrillated cellulose	569:593	nanofibrillated cellulose (CNF)	569:599	Three nanocomposite films were fabricated via the casting process: nanofibrillated cellulose (CNF), nanocellulose-PVA (CNF-P) and nanocellulose-silica (CNF-Si).
28602986	7	6	theme	imaginary	940:948	arg1	parts					950:954	The real and imaginary parts	927:954	The real and imaginary parts of permittivity and electric modulus	927:991	The real and imaginary parts of permittivity and electric modulus were evaluated using an impedance analyzer.
28602986	10	7	theme	temperature	1273:1283	arg1	interval					1285:1292	a high temperature interval	1266:1292	a high temperature interval	1266:1292	At a high temperature interval, the storage modulus of the CNF-Si was greater than that of the CNF or CNF-P.
28602986	0	8	theme	Celluclast-treated	88:105	arg1	composites					133:142	Celluclast-treated nanofibrillated cellulose composites	88:142	Celluclast-treated nanofibrillated cellulose composites	88:142	Influence of PVA and silica on chemical, thermo-mechanical and electrical properties of Celluclast-treated nanofibrillated cellulose composites.
28602986	0	9	from	Influence	0:8	arg1	properties					74:83	chemical, thermo-mechanical and electrical properties	31:83	chemical, thermo-mechanical and electrical properties of Celluclast-treated nanofibrillated cellulose composites	31:142	Influence of PVA and silica on chemical, thermo-mechanical and electrical properties of Celluclast-treated nanofibrillated cellulose composites.
28602986	1	10	from	effects	171:177	arg1	properties					246:255	properties	246:255	properties of Celluclast-treated nanofibrillated cellulose composites	246:314	This study reports on the effects of organic polyvinyl alcohol (PVA) and inorganic silica polymer on properties of Celluclast-treated nanofibrillated cellulose composites.
28602986	2	11	theme	Nanofibrillated	317:331	arg1	cellulose					333:341	Nanofibrillated cellulose	317:341	Nanofibrillated cellulose	317:341	Nanofibrillated cellulose was isolated from Eucalyptus camaldulensis and prior to high-pressure homogenizing was pretreated with Celluclast enzyme in order to lower energy consumption.
28602986	1	12	theme	Celluclast-treated	260:277	arg1	composites					305:314	Celluclast-treated nanofibrillated cellulose composites	260:314	Celluclast-treated nanofibrillated cellulose composites	260:314	This study reports on the effects of organic polyvinyl alcohol (PVA) and inorganic silica polymer on properties of Celluclast-treated nanofibrillated cellulose composites.
28602986	8	13	theme	nanocomposites	1069:1082	arg1	values					1055:1060	The crystallinity values	1037:1060	The crystallinity values of the nanocomposites calculated from the FT-IR	1037:1108	The crystallinity values of the nanocomposites calculated from the FT-IR were in agreement with the TGA results, showing that the lowest crystallinity value was in the CNF-Si.
28602986	7	14	theme	real	931:934	arg1	parts					950:954	The real and imaginary parts	927:954	The real and imaginary parts of permittivity and electric modulus	927:991	The real and imaginary parts of permittivity and electric modulus were evaluated using an impedance analyzer.
28602986	1	15	theme	nanofibrillated	279:293	arg1	composites					305:314	Celluclast-treated nanofibrillated cellulose composites	260:314	Celluclast-treated nanofibrillated cellulose composites	260:314	This study reports on the effects of organic polyvinyl alcohol (PVA) and inorganic silica polymer on properties of Celluclast-treated nanofibrillated cellulose composites.
28602986	0	16	theme	cellulose	123:131	arg1	composites					133:142	Celluclast-treated nanofibrillated cellulose composites	88:142	Celluclast-treated nanofibrillated cellulose composites	88:142	Influence of PVA and silica on chemical, thermo-mechanical and electrical properties of Celluclast-treated nanofibrillated cellulose composites.
28602986	8	17	theme	TGA	1137:1139	arg1	results					1141:1147	the TGA results	1133:1147	the TGA results	1133:1147	The crystallinity values of the nanocomposites calculated from the FT-IR were in agreement with the TGA results, showing that the lowest crystallinity value was in the CNF-Si.
28602986	10	18	theme	CNF-Si	1322:1327	arg1	greater					1333:1339	greater	1333:1339	greater	1333:1339	At a high temperature interval, the storage modulus of the CNF-Si was greater than that of the CNF or CNF-P.
28602986	10	18	theme	CNF-Si	1322:1327	arg1	modulus					1307:1313	the storage modulus	1295:1313	the storage modulus of the CNF-Si	1295:1327	At a high temperature interval, the storage modulus of the CNF-Si was greater than that of the CNF or CNF-P.
28602986	8	19	with	agreement	1118:1126	arg1	results					1141:1147	the TGA results	1133:1147	the TGA results	1133:1147	The crystallinity values of the nanocomposites calculated from the FT-IR were in agreement with the TGA results, showing that the lowest crystallinity value was in the CNF-Si.
28602986	0	20	theme	nanofibrillated	107:121	arg1	composites					133:142	Celluclast-treated nanofibrillated cellulose composites	88:142	Celluclast-treated nanofibrillated cellulose composites	88:142	Influence of PVA and silica on chemical, thermo-mechanical and electrical properties of Celluclast-treated nanofibrillated cellulose composites.
28602986	6	21	theme	nanocomposites	854:867	arg1	's					825:826	The Young's	816:826	The Young's	816:826	The Young's and storage moduli of the nanocomposites were determined via a universal testing machine and DTMA.
28602986	6	21	theme	nanocomposites	854:867	arg1	moduli					840:845	storage moduli	832:845	storage moduli	832:845	The Young's and storage moduli of the nanocomposites were determined via a universal testing machine and DTMA.
28602986	10	22	theme	high	1268:1271	arg1	interval					1285:1292	a high temperature interval	1266:1292	a high temperature interval	1266:1292	At a high temperature interval, the storage modulus of the CNF-Si was greater than that of the CNF or CNF-P.
28602986	11	23	theme	CNF-P	1461:1465	arg1	processes					1467:1475	the CNF and the CNF-P processes	1445:1475	the CNF and the CNF-P processes	1445:1475	The CNF-Si also exhibited a completed singular relaxation process, while the CNF and the CNF-P processes were uncompleted.
28602986	1	24	theme	organic	182:188	arg1	PVA					209:211	PVA	209:211	PVA	209:211	This study reports on the effects of organic polyvinyl alcohol (PVA) and inorganic silica polymer on properties of Celluclast-treated nanofibrillated cellulose composites.
28602986	1	24	theme	organic	182:188	arg1	alcohol					200:206	organic polyvinyl alcohol	182:206	organic polyvinyl alcohol (PVA)	182:212	This study reports on the effects of organic polyvinyl alcohol (PVA) and inorganic silica polymer on properties of Celluclast-treated nanofibrillated cellulose composites.
28602986	1	25	theme	cellulose	295:303	arg1	composites					305:314	Celluclast-treated nanofibrillated cellulose composites	260:314	Celluclast-treated nanofibrillated cellulose composites	260:314	This study reports on the effects of organic polyvinyl alcohol (PVA) and inorganic silica polymer on properties of Celluclast-treated nanofibrillated cellulose composites.
28602986	1	26	theme	polyvinyl	190:198	arg1	PVA					209:211	PVA	209:211	PVA	209:211	This study reports on the effects of organic polyvinyl alcohol (PVA) and inorganic silica polymer on properties of Celluclast-treated nanofibrillated cellulose composites.
28602986	1	26	theme	polyvinyl	190:198	arg1	alcohol					200:206	organic polyvinyl alcohol	182:206	organic polyvinyl alcohol (PVA)	182:212	This study reports on the effects of organic polyvinyl alcohol (PVA) and inorganic silica polymer on properties of Celluclast-treated nanofibrillated cellulose composites.
28602986	2	27	attach	isolated	347:354	arg2	cellulose					333:341	Nanofibrillated cellulose	317:341	Nanofibrillated cellulose	317:341	Nanofibrillated cellulose was isolated from Eucalyptus camaldulensis and prior to high-pressure homogenizing was pretreated with Celluclast enzyme in order to lower energy consumption.
28602986	2	27	attach	isolated	347:354	arg1	camaldulensis					372:384	Eucalyptus camaldulensis	361:384	Eucalyptus camaldulensis	361:384	Nanofibrillated cellulose was isolated from Eucalyptus camaldulensis and prior to high-pressure homogenizing was pretreated with Celluclast enzyme in order to lower energy consumption.
28602986	12	28	theme	industrial	1521:1530	arg1	applications					1532:1543	industrial applications	1521:1543	industrial applications	1521:1543	Consequently, in terms of industrial applications, although the CNF-P composite had mechanical advantages, the CNF-Si composite displayed the best thermo-mechanical properties.
28602986	1	29	theme	alcohol	200:206	arg1	effects					171:177	the effects	167:177	the effects of organic polyvinyl alcohol (PVA) and inorganic silica polymer on properties of Celluclast-treated nanofibrillated cellulose composites	167:314	This study reports on the effects of organic polyvinyl alcohol (PVA) and inorganic silica polymer on properties of Celluclast-treated nanofibrillated cellulose composites.
28602986	1	30	theme	composites	305:314	arg1	properties					246:255	properties	246:255	properties of Celluclast-treated nanofibrillated cellulose composites	246:314	This study reports on the effects of organic polyvinyl alcohol (PVA) and inorganic silica polymer on properties of Celluclast-treated nanofibrillated cellulose composites.
28602986	12	31	contain	had	1575:1577	arg1	composite					1565:1573	the CNF-P composite	1555:1573	the CNF-P composite	1555:1573	Consequently, in terms of industrial applications, although the CNF-P composite had mechanical advantages, the CNF-Si composite displayed the best thermo-mechanical properties.
28602986	12	31	contain	had	1575:1577	arg2	advantages					1590:1599	mechanical advantages	1579:1599	mechanical advantages	1579:1599	Consequently, in terms of industrial applications, although the CNF-P composite had mechanical advantages, the CNF-Si composite displayed the best thermo-mechanical properties.
28602986	9	32	theme	tensile	1245:1251	arg1	strength					1253:1260	the highest tensile strength	1233:1260	the highest tensile strength	1233:1260	The CNF-P displayed the highest tensile strength.
28602986	2	33	theme	lower	476:480	arg1	consumption					489:499	lower energy consumption	476:499	lower energy consumption	476:499	Nanofibrillated cellulose was isolated from Eucalyptus camaldulensis and prior to high-pressure homogenizing was pretreated with Celluclast enzyme in order to lower energy consumption.
28602986	0	34	theme	PVA	13:15	arg1	Influence					0:8	Influence	0:8	Influence of PVA and silica on chemical, thermo-mechanical and electrical properties of Celluclast-treated nanofibrillated cellulose composites.	0:143	Influence of PVA and silica on chemical, thermo-mechanical and electrical properties of Celluclast-treated nanofibrillated cellulose composites.
28602986	0	35	theme	composites	133:142	arg1	properties					74:83	chemical, thermo-mechanical and electrical properties	31:83	chemical, thermo-mechanical and electrical properties of Celluclast-treated nanofibrillated cellulose composites	31:142	Influence of PVA and silica on chemical, thermo-mechanical and electrical properties of Celluclast-treated nanofibrillated cellulose composites.
28602986	11	36	theme	completed	1400:1408	arg1	process					1430:1436	a completed singular relaxation process	1398:1436	a completed singular relaxation process	1398:1436	The CNF-Si also exhibited a completed singular relaxation process, while the CNF and the CNF-P processes were uncompleted.
28602986	8	37	theme	lowest	1167:1172	arg1	value					1188:1192	the lowest crystallinity value	1163:1192	the lowest crystallinity value	1163:1192	The crystallinity values of the nanocomposites calculated from the FT-IR were in agreement with the TGA results, showing that the lowest crystallinity value was in the CNF-Si.
28602986	7	38	theme	electric	976:983	arg1	modulus					985:991	electric modulus	976:991	electric modulus	976:991	The real and imaginary parts of permittivity and electric modulus were evaluated using an impedance analyzer.
28602986	3	39	theme	nanocomposite	508:520	arg1	films					522:526	Three nanocomposite films	502:526	Three nanocomposite films	502:526	Three nanocomposite films were fabricated via the casting process: nanofibrillated cellulose (CNF), nanocellulose-PVA (CNF-P) and nanocellulose-silica (CNF-Si).
28602986	0	40	theme	silica	21:26	arg1	Influence					0:8	Influence	0:8	Influence of PVA and silica on chemical, thermo-mechanical and electrical properties of Celluclast-treated nanofibrillated cellulose composites.	0:143	Influence of PVA and silica on chemical, thermo-mechanical and electrical properties of Celluclast-treated nanofibrillated cellulose composites.
28602986	11	41	theme	singular	1410:1417	arg1	process					1430:1436	a completed singular relaxation process	1398:1436	a completed singular relaxation process	1398:1436	The CNF-Si also exhibited a completed singular relaxation process, while the CNF and the CNF-P processes were uncompleted.
28602986	12	42	theme	CNF-P	1559:1563	arg1	composite					1565:1573	the CNF-P composite	1555:1573	the CNF-P composite	1555:1573	Consequently, in terms of industrial applications, although the CNF-P composite had mechanical advantages, the CNF-Si composite displayed the best thermo-mechanical properties.
28602986	11	43	theme	CNF	1449:1451	arg1	processes					1467:1475	the CNF and the CNF-P processes	1445:1475	the CNF and the CNF-P processes	1445:1475	The CNF-Si also exhibited a completed singular relaxation process, while the CNF and the CNF-P processes were uncompleted.
28602986	10	44	dep	CNF	1358:1360	arg1	the					1354:1356	the	1354:1356	the	1354:1356	At a high temperature interval, the storage modulus of the CNF-Si was greater than that of the CNF or CNF-P.
28602986	4	45	theme	thermal	710:716	arg1	stability					718:726	thermal stability	710:726	thermal stability	710:726	Chemical characterization, crystallization and thermal stability were determined using FT-IR and TGA.
28602986	6	46	theme	storage	832:838	arg1	moduli					840:845	storage moduli	832:845	storage moduli	832:845	The Young's and storage moduli of the nanocomposites were determined via a universal testing machine and DTMA.
28602986	3	47	theme	casting	552:558	arg1	process					560:566	the casting process	548:566	the casting process	548:566	Three nanocomposite films were fabricated via the casting process: nanofibrillated cellulose (CNF), nanocellulose-PVA (CNF-P) and nanocellulose-silica (CNF-Si).
28602986	0	48	theme	chemical	31:38	arg1	properties					74:83	chemical, thermo-mechanical and electrical properties	31:83	chemical, thermo-mechanical and electrical properties of Celluclast-treated nanofibrillated cellulose composites	31:142	Influence of PVA and silica on chemical, thermo-mechanical and electrical properties of Celluclast-treated nanofibrillated cellulose composites.
28602986	12	49	theme	thermo-mechanical	1642:1658	arg1	properties					1660:1669	the best thermo-mechanical properties	1633:1669	the best thermo-mechanical properties	1633:1669	Consequently, in terms of industrial applications, although the CNF-P composite had mechanical advantages, the CNF-Si composite displayed the best thermo-mechanical properties.
28602986	8	50	theme	crystallinity	1174:1186	arg1	value					1188:1192	the lowest crystallinity value	1163:1192	the lowest crystallinity value	1163:1192	The crystallinity values of the nanocomposites calculated from the FT-IR were in agreement with the TGA results, showing that the lowest crystallinity value was in the CNF-Si.
28602986	7	51	theme	modulus	985:991	arg1	parts					950:954	The real and imaginary parts	927:954	The real and imaginary parts of permittivity and electric modulus	927:991	The real and imaginary parts of permittivity and electric modulus were evaluated using an impedance analyzer.
28602986	12	52	theme	best	1637:1640	arg1	properties					1660:1669	the best thermo-mechanical properties	1633:1669	the best thermo-mechanical properties	1633:1669	Consequently, in terms of industrial applications, although the CNF-P composite had mechanical advantages, the CNF-Si composite displayed the best thermo-mechanical properties.
28602986	10	53	theme	storage	1299:1305	arg1	greater					1333:1339	greater	1333:1339	greater	1333:1339	At a high temperature interval, the storage modulus of the CNF-Si was greater than that of the CNF or CNF-P.
28602986	10	53	theme	storage	1299:1305	arg1	modulus					1307:1313	the storage modulus	1295:1313	the storage modulus of the CNF-Si	1295:1327	At a high temperature interval, the storage modulus of the CNF-Si was greater than that of the CNF or CNF-P.
28602986	1	54	theme	inorganic	218:226	arg1	polymer					235:241	inorganic silica polymer	218:241	inorganic silica polymer	218:241	This study reports on the effects of organic polyvinyl alcohol (PVA) and inorganic silica polymer on properties of Celluclast-treated nanofibrillated cellulose composites.
28602986	5	55	theme	Morphological	765:777	arg1	alterations					779:789	Morphological alterations	765:789	Morphological alterations	765:789	Morphological alterations were monitored with SEM.
28602986	12	56	theme	applications	1532:1543	arg1	terms					1512:1516	terms	1512:1516	terms of industrial applications	1512:1543	Consequently, in terms of industrial applications, although the CNF-P composite had mechanical advantages, the CNF-Si composite displayed the best thermo-mechanical properties.
28602986	6	57	theme	Young	820:824	arg1	's					825:826	The Young's	816:826	The Young's	816:826	The Young's and storage moduli of the nanocomposites were determined via a universal testing machine and DTMA.
28602986	7	58	theme	impedance	1017:1025	arg1	analyzer					1027:1034	an impedance analyzer	1014:1034	an impedance analyzer	1014:1034	The real and imaginary parts of permittivity and electric modulus were evaluated using an impedance analyzer.
28602986	1	59	theme	silica	228:233	arg1	polymer					235:241	inorganic silica polymer	218:241	inorganic silica polymer	218:241	This study reports on the effects of organic polyvinyl alcohol (PVA) and inorganic silica polymer on properties of Celluclast-treated nanofibrillated cellulose composites.
28602986	6	60	theme	testing	901:907	arg1	machine					909:915	a universal testing machine	889:915	a universal testing machine	889:915	The Young's and storage moduli of the nanocomposites were determined via a universal testing machine and DTMA.
28602986	12	61	theme	mechanical	1579:1588	arg1	advantages					1590:1599	mechanical advantages	1579:1599	mechanical advantages	1579:1599	Consequently, in terms of industrial applications, although the CNF-P composite had mechanical advantages, the CNF-Si composite displayed the best thermo-mechanical properties.
28602986	2	62	theme	Celluclast	446:455	arg1	enzyme					457:462	Celluclast enzyme	446:462	Celluclast enzyme	446:462	Nanofibrillated cellulose was isolated from Eucalyptus camaldulensis and prior to high-pressure homogenizing was pretreated with Celluclast enzyme in order to lower energy consumption.
28602986	1	63	theme	polymer	235:241	arg1	effects					171:177	the effects	167:177	the effects of organic polyvinyl alcohol (PVA) and inorganic silica polymer on properties of Celluclast-treated nanofibrillated cellulose composites	167:314	This study reports on the effects of organic polyvinyl alcohol (PVA) and inorganic silica polymer on properties of Celluclast-treated nanofibrillated cellulose composites.
28602986	0	64	theme	thermo-mechanical	41:57	arg1	properties					74:83	chemical, thermo-mechanical and electrical properties	31:83	chemical, thermo-mechanical and electrical properties of Celluclast-treated nanofibrillated cellulose composites	31:142	Influence of PVA and silica on chemical, thermo-mechanical and electrical properties of Celluclast-treated nanofibrillated cellulose composites.
28602986	2	65	theme	energy	482:487	arg1	consumption					489:499	lower energy consumption	476:499	lower energy consumption	476:499	Nanofibrillated cellulose was isolated from Eucalyptus camaldulensis and prior to high-pressure homogenizing was pretreated with Celluclast enzyme in order to lower energy consumption.
28602986	8	66	theme	crystallinity	1041:1053	arg1	values					1055:1060	The crystallinity values	1037:1060	The crystallinity values of the nanocomposites calculated from the FT-IR	1037:1108	The crystallinity values of the nanocomposites calculated from the FT-IR were in agreement with the TGA results, showing that the lowest crystallinity value was in the CNF-Si.
28602986	4	67	theme	Chemical	663:670	arg1	characterization					672:687	Chemical characterization	663:687	Chemical characterization	663:687	Chemical characterization, crystallization and thermal stability were determined using FT-IR and TGA.
28602986	12	68	theme	CNF-Si	1606:1611	arg1	composite					1613:1621	the CNF-Si composite	1602:1621	the CNF-Si composite	1602:1621	Consequently, in terms of industrial applications, although the CNF-P composite had mechanical advantages, the CNF-Si composite displayed the best thermo-mechanical properties.
24423535	2	0	theme	centesimal	311:320	arg1	composition					322:332	their centesimal composition	305:332	their centesimal composition	305:332	The dietetic use of the flour made from fruit seeds depends on their centesimal composition and other characteristics.
24423535	0	1	theme	fruit	80:84	arg1	seeds					86:90	fruit seeds	80:90	fruit seeds	80:90	Determination of the centesimal composition and characterization of flours from fruit seeds.
24423535	8	2	theme	cherry	924:929	arg1	seeds					931:935	cherry seeds	924:935	cherry seeds	924:935	The X-ray diffraction analyses showed that the starches from jackfruit and Surinam cherry seeds presented A-type crystallinity and the thermogravimetric tests showed they were the most thermally resistant.
24423535	6	3	theme	Surinam	757:763	arg1	%					754:754	3%	753:754	3% (Surinam cherry)	753:771	The lipid content varied from 3% (Surinam cherry) to 39% (peach).
24423535	6	3	theme	Surinam	757:763	arg1	cherry					765:770	Surinam cherry	757:770	Surinam cherry	757:770	The lipid content varied from 3% (Surinam cherry) to 39% (peach).
24423535	1	4	theme	human	230:234	arg1	diet					236:239	the human diet	226:239	the human diet	226:239	Fruit seeds, common byproducts of the food industry, are generally discarded despite their potential use as a source of nutrients in the human diet.
24423535	9	5	theme	hydrogen	1078:1085	arg1	nucleus					1087:1093	the hydrogen nucleus	1074:1093	the hydrogen nucleus	1074:1093	The relaxometry studies of the hydrogen nucleus determined the mobility domains of each sample.
24423535	5	6	theme	seeds	648:652	arg1	content					633:639	The protein content	621:639	The protein content of the seeds	621:652	The protein content of the seeds ranged from a low of 10% (Surinam cherry) to a high of 32% (orange).
24423535	9	7	theme	relaxometry	1051:1061	arg1	studies					1063:1069	The relaxometry studies	1047:1069	The relaxometry studies of the hydrogen nucleus	1047:1093	The relaxometry studies of the hydrogen nucleus determined the mobility domains of each sample.
24423535	6	8	theme	lipid	727:731	arg1	content					733:739	The lipid content	723:739	The lipid content	723:739	The lipid content varied from 3% (Surinam cherry) to 39% (peach).
24423535	6	9	dep	varied	741:746	arg1	peach					781:785	peach	781:785	peach	781:785	The lipid content varied from 3% (Surinam cherry) to 39% (peach).
24423535	0	10	from	characterization	48:63	arg1	seeds					86:90	fruit seeds	80:90	fruit seeds	80:90	Determination of the centesimal composition and characterization of flours from fruit seeds.
24423535	2	11	theme	dietetic	246:253	arg1	use					255:257	The dietetic use	242:257	The dietetic use of the flour made from fruit seeds	242:292	The dietetic use of the flour made from fruit seeds depends on their centesimal composition and other characteristics.
24423535	8	12	theme	diffraction	851:861	arg1	analyses					863:870	The X-ray diffraction analyses	841:870	The X-ray diffraction analyses	841:870	The X-ray diffraction analyses showed that the starches from jackfruit and Surinam cherry seeds presented A-type crystallinity and the thermogravimetric tests showed they were the most thermally resistant.
24423535	0	13	from	composition	32:42	arg1	seeds					86:90	fruit seeds	80:90	fruit seeds	80:90	Determination of the centesimal composition and characterization of flours from fruit seeds.
24423535	5	14	theme	protein	625:631	arg1	content					633:639	The protein content	621:639	The protein content of the seeds	621:652	The protein content of the seeds ranged from a low of 10% (Surinam cherry) to a high of 32% (orange).
24423535	8	15	theme	X-ray	845:849	arg1	diffraction					851:861	The X-ray diffraction	841:861	The X-ray diffraction analyses	841:870	The X-ray diffraction analyses showed that the starches from jackfruit and Surinam cherry seeds presented A-type crystallinity and the thermogravimetric tests showed they were the most thermally resistant.
24423535	4	16	theme	X-ray	532:536	arg1	diffraction					538:548	X-ray diffraction	532:548	X-ray diffraction	532:548	The flours obtained from these seeds were characterized by infrared absorption spectrometry, X-ray diffraction, thermogravimetric analysis, and low-field nuclear magnetic resonance.
24423535	8	17	from	jackfruit	902:910	arg1	starches					888:895	the starches	884:895	the starches from jackfruit and Surinam cherry seeds	884:935	The X-ray diffraction analyses showed that the starches from jackfruit and Surinam cherry seeds presented A-type crystallinity and the thermogravimetric tests showed they were the most thermally resistant.
24423535	7	18	theme	ash	801:803	arg1	content					805:811	The highest ash content	789:811	The highest ash content	789:811	The highest ash content was 3.9% (melon and peach).
24423535	7	18	theme	ash	801:803	arg1	%					820:820	3.9%	817:820	3.9% (melon and peach)	817:838	The highest ash content was 3.9% (melon and peach).
24423535	1	19	theme	Fruit	93:97	arg1	seeds					99:103	Fruit seeds	93:103	Fruit seeds	93:103	Fruit seeds, common byproducts of the food industry, are generally discarded despite their potential use as a source of nutrients in the human diet.
24423535	1	19	theme	Fruit	93:97	arg1	byproducts					113:122	common byproducts	106:122	common byproducts of the food industry	106:143	Fruit seeds, common byproducts of the food industry, are generally discarded despite their potential use as a source of nutrients in the human diet.
24423535	2	20	theme	other	338:342	arg1	characteristics					344:358	other characteristics	338:358	other characteristics	338:358	The dietetic use of the flour made from fruit seeds depends on their centesimal composition and other characteristics.
24423535	0	21	theme	centesimal	21:30	arg1	composition					32:42	centesimal composition	21:42	centesimal composition	21:42	Determination of the centesimal composition and characterization of flours from fruit seeds.
24423535	9	22	theme	nucleus	1087:1093	arg1	studies					1063:1069	The relaxometry studies	1047:1069	The relaxometry studies of the hydrogen nucleus	1047:1093	The relaxometry studies of the hydrogen nucleus determined the mobility domains of each sample.
24423535	2	23	theme	flour	266:270	arg1	use					255:257	The dietetic use	242:257	The dietetic use of the flour made from fruit seeds	242:292	The dietetic use of the flour made from fruit seeds depends on their centesimal composition and other characteristics.
24423535	1	24	theme	potential	184:192	arg1	use					194:196	their potential use	178:196	their potential use as a source of nutrients in the human diet	178:239	Fruit seeds, common byproducts of the food industry, are generally discarded despite their potential use as a source of nutrients in the human diet.
24423535	0	25	from	seeds	86:90	arg1	flours					68:73	flours	68:73	flours from fruit seeds	68:90	Determination of the centesimal composition and characterization of flours from fruit seeds.
24423535	0	25	from	seeds	86:90	arg1	characterization					48:63	characterization	48:63	characterization	48:63	Determination of the centesimal composition and characterization of flours from fruit seeds.
24423535	0	25	from	seeds	86:90	arg1	composition					32:42	centesimal composition	21:42	centesimal composition	21:42	Determination of the centesimal composition and characterization of flours from fruit seeds.
24423535	4	26	theme	thermogravimetric	551:567	arg1	analysis					569:576	thermogravimetric analysis	551:576	thermogravimetric analysis	551:576	The flours obtained from these seeds were characterized by infrared absorption spectrometry, X-ray diffraction, thermogravimetric analysis, and low-field nuclear magnetic resonance.
24423535	9	27	theme	mobility	1110:1117	arg1	domains					1119:1125	the mobility domains	1106:1125	the mobility domains of each sample	1106:1140	The relaxometry studies of the hydrogen nucleus determined the mobility domains of each sample.
24423535	9	27	theme	mobility	1110:1117	arg1	sample					1135:1140	each sample	1130:1140	each sample	1130:1140	The relaxometry studies of the hydrogen nucleus determined the mobility domains of each sample.
24423535	5	28	theme	Surinam	680:686	arg1	%					677:677	a low of 10%	666:677	a low of 10% (Surinam cherry)	666:694	The protein content of the seeds ranged from a low of 10% (Surinam cherry) to a high of 32% (orange).
24423535	5	28	theme	Surinam	680:686	arg1	cherry					688:693	Surinam cherry	680:693	Surinam cherry	680:693	The protein content of the seeds ranged from a low of 10% (Surinam cherry) to a high of 32% (orange).
24423535	0	29	theme	composition	32:42	arg1	Determination					0:12	Determination	0:12	Determination of the centesimal composition and characterization of flours from fruit seeds.	0:91	Determination of the centesimal composition and characterization of flours from fruit seeds.
24423535	4	30	theme	infrared	498:505	arg1	spectrometry					518:529	infrared absorption spectrometry	498:529	infrared absorption spectrometry	498:529	The flours obtained from these seeds were characterized by infrared absorption spectrometry, X-ray diffraction, thermogravimetric analysis, and low-field nuclear magnetic resonance.
24423535	3	31	theme	centesimal	378:387	arg1	compositions					389:400	the centesimal compositions	374:400	the centesimal compositions	374:400	In this work the centesimal compositions were determined of six fruits seeds.
24423535	8	32	dep	showed	1000:1005	arg1	resistant					1036:1044	resistant	1036:1044	resistant	1036:1044	The X-ray diffraction analyses showed that the starches from jackfruit and Surinam cherry seeds presented A-type crystallinity and the thermogravimetric tests showed they were the most thermally resistant.
24423535	3	33	theme	fruits	425:430	arg1	seeds					432:436	six fruits seeds	421:436	six fruits seeds	421:436	In this work the centesimal compositions were determined of six fruits seeds.
24423535	8	34	from	Surinam	916:922	arg1	starches					888:895	the starches	884:895	the starches from jackfruit and Surinam cherry seeds	884:935	The X-ray diffraction analyses showed that the starches from jackfruit and Surinam cherry seeds presented A-type crystallinity and the thermogravimetric tests showed they were the most thermally resistant.
24423535	5	35	theme	of	706:707	arg1	orange					714:719	orange	714:719	orange	714:719	The protein content of the seeds ranged from a low of 10% (Surinam cherry) to a high of 32% (orange).
24423535	5	35	theme	of	706:707	arg1	%					711:711	a high of 32%	699:711	a high of 32% (orange)	699:720	The protein content of the seeds ranged from a low of 10% (Surinam cherry) to a high of 32% (orange).
24423535	1	36	theme	common	106:111	arg1	seeds					99:103	Fruit seeds	93:103	Fruit seeds	93:103	Fruit seeds, common byproducts of the food industry, are generally discarded despite their potential use as a source of nutrients in the human diet.
24423535	1	36	theme	common	106:111	arg1	byproducts					113:122	common byproducts	106:122	common byproducts of the food industry	106:143	Fruit seeds, common byproducts of the food industry, are generally discarded despite their potential use as a source of nutrients in the human diet.
24423535	0	37	theme	characterization	48:63	arg1	Determination					0:12	Determination	0:12	Determination of the centesimal composition and characterization of flours from fruit seeds.	0:91	Determination of the centesimal composition and characterization of flours from fruit seeds.
24423535	0	38	dep	composition	32:42	arg1	the					17:19	the	17:19	the	17:19	Determination of the centesimal composition and characterization of flours from fruit seeds.
24423535	7	39	dep	%	820:820	arg1	peach					833:837	peach	833:837	peach	833:837	The highest ash content was 3.9% (melon and peach).
24423535	7	39	dep	%	820:820	arg1	melon					823:827	melon	823:827	melon	823:827	The highest ash content was 3.9% (melon and peach).
24423535	4	40	theme	absorption	507:516	arg1	spectrometry					518:529	infrared absorption spectrometry	498:529	infrared absorption spectrometry	498:529	The flours obtained from these seeds were characterized by infrared absorption spectrometry, X-ray diffraction, thermogravimetric analysis, and low-field nuclear magnetic resonance.
24423535	1	41	from	source	203:208	arg1	diet					236:239	the human diet	226:239	the human diet	226:239	Fruit seeds, common byproducts of the food industry, are generally discarded despite their potential use as a source of nutrients in the human diet.
24423535	9	42	theme	sample	1135:1140	arg1	domains					1119:1125	the mobility domains	1106:1125	the mobility domains of each sample	1106:1140	The relaxometry studies of the hydrogen nucleus determined the mobility domains of each sample.
24423535	9	42	theme	sample	1135:1140	arg1	sample					1135:1140	each sample	1130:1140	each sample	1130:1140	The relaxometry studies of the hydrogen nucleus determined the mobility domains of each sample.
24423535	8	43	dep	jackfruit	902:910	arg1	seeds					931:935	cherry seeds	924:935	cherry seeds	924:935	The X-ray diffraction analyses showed that the starches from jackfruit and Surinam cherry seeds presented A-type crystallinity and the thermogravimetric tests showed they were the most thermally resistant.
24423535	7	44	theme	highest	793:799	arg1	content					805:811	The highest ash content	789:811	The highest ash content	789:811	The highest ash content was 3.9% (melon and peach).
24423535	7	44	theme	highest	793:799	arg1	%					820:820	3.9%	817:820	3.9% (melon and peach)	817:838	The highest ash content was 3.9% (melon and peach).
24423535	4	45	theme	nuclear	593:599	arg1	resonance					610:618	low-field nuclear magnetic resonance	583:618	low-field nuclear magnetic resonance	583:618	The flours obtained from these seeds were characterized by infrared absorption spectrometry, X-ray diffraction, thermogravimetric analysis, and low-field nuclear magnetic resonance.
24423535	8	46	theme	thermogravimetric	976:992	arg1	tests					994:998	the thermogravimetric tests	972:998	the thermogravimetric tests	972:998	The X-ray diffraction analyses showed that the starches from jackfruit and Surinam cherry seeds presented A-type crystallinity and the thermogravimetric tests showed they were the most thermally resistant.
24423535	2	47	theme	fruit	282:286	arg1	seeds					288:292	fruit seeds	282:292	fruit seeds	282:292	The dietetic use of the flour made from fruit seeds depends on their centesimal composition and other characteristics.
24423535	4	48	theme	low-field	583:591	arg1	resonance					610:618	low-field nuclear magnetic resonance	583:618	low-field nuclear magnetic resonance	583:618	The flours obtained from these seeds were characterized by infrared absorption spectrometry, X-ray diffraction, thermogravimetric analysis, and low-field nuclear magnetic resonance.
24423535	5	49	theme	high	701:704	arg1	orange					714:719	orange	714:719	orange	714:719	The protein content of the seeds ranged from a low of 10% (Surinam cherry) to a high of 32% (orange).
24423535	5	49	theme	high	701:704	arg1	%					711:711	a high of 32%	699:711	a high of 32% (orange)	699:720	The protein content of the seeds ranged from a low of 10% (Surinam cherry) to a high of 32% (orange).
24423535	1	50	theme	food	131:134	arg1	industry					136:143	the food industry	127:143	the food industry	127:143	Fruit seeds, common byproducts of the food industry, are generally discarded despite their potential use as a source of nutrients in the human diet.
24423535	1	51	theme	nutrients	213:221	arg1	source					203:208	a source	201:208	a source of nutrients in the human diet	201:239	Fruit seeds, common byproducts of the food industry, are generally discarded despite their potential use as a source of nutrients in the human diet.
24423535	0	52	theme	flours	68:73	arg1	characterization					48:63	characterization	48:63	characterization	48:63	Determination of the centesimal composition and characterization of flours from fruit seeds.
24423535	0	52	theme	flours	68:73	arg1	composition					32:42	centesimal composition	21:42	centesimal composition	21:42	Determination of the centesimal composition and characterization of flours from fruit seeds.
24423535	1	53	theme	industry	136:143	arg1	seeds					99:103	Fruit seeds	93:103	Fruit seeds	93:103	Fruit seeds, common byproducts of the food industry, are generally discarded despite their potential use as a source of nutrients in the human diet.
24423535	1	53	theme	industry	136:143	arg1	byproducts					113:122	common byproducts	106:122	common byproducts of the food industry	106:143	Fruit seeds, common byproducts of the food industry, are generally discarded despite their potential use as a source of nutrients in the human diet.
24423535	8	54	theme	A-type	947:952	arg1	crystallinity					954:966	A-type crystallinity	947:966	A-type crystallinity	947:966	The X-ray diffraction analyses showed that the starches from jackfruit and Surinam cherry seeds presented A-type crystallinity and the thermogravimetric tests showed they were the most thermally resistant.
24423535	4	55	theme	magnetic	601:608	arg1	resonance					610:618	low-field nuclear magnetic resonance	583:618	low-field nuclear magnetic resonance	583:618	The flours obtained from these seeds were characterized by infrared absorption spectrometry, X-ray diffraction, thermogravimetric analysis, and low-field nuclear magnetic resonance.
26920320	0	0	theme	fructo-oligosaccharides	92:114	arg1	composition					39:49	the composition	35:49	the composition of enzymatically synthesized short-chain fructo-oligosaccharides	35:114	Effect of sucrose concentration on the composition of enzymatically synthesized short-chain fructo-oligosaccharides as determined by FTIR and multivariate analysis.
26920320	2	1	theme	reaction	568:575	arg1	medium					577:582	the reaction medium	564:582	the reaction medium directly from the FTIR spectra	564:613	As their composition is determined by the synthesis conditions, the goals of this work were: (a) to engineer FOS of different composition by adjusting the sucrose concentration used as initial substrate; (b) to define partial least square (PLS) based-models to quantify all the sugars present in the reaction medium directly from the FTIR spectra.
26920320	0	2	theme	short-chain	80:90	arg1	fructo-oligosaccharides					92:114	enzymatically synthesized short-chain fructo-oligosaccharides	54:114	enzymatically synthesized short-chain fructo-oligosaccharides	54:114	Effect of sucrose concentration on the composition of enzymatically synthesized short-chain fructo-oligosaccharides as determined by FTIR and multivariate analysis.
26920320	2	3	attach	present	553:559	arg2	sugars					546:551	all the sugars	538:551	all the sugars present in the reaction medium directly from the FTIR spectra	538:613	As their composition is determined by the synthesis conditions, the goals of this work were: (a) to engineer FOS of different composition by adjusting the sucrose concentration used as initial substrate; (b) to define partial least square (PLS) based-models to quantify all the sugars present in the reaction medium directly from the FTIR spectra.
26920320	2	3	attach	present	553:559	arg1	medium					577:582	the reaction medium	564:582	the reaction medium directly from the FTIR spectra	564:613	As their composition is determined by the synthesis conditions, the goals of this work were: (a) to engineer FOS of different composition by adjusting the sucrose concentration used as initial substrate; (b) to define partial least square (PLS) based-models to quantify all the sugars present in the reaction medium directly from the FTIR spectra.
26920320	7	4	theme	FTIR	1077:1080	arg1	spectra					1082:1088	the FTIR spectra	1073:1088	the FTIR spectra	1073:1088	Determining FOS composition directly from the FTIR spectra represents a useful tool to monitor enzymatic synthesis, with strong impact at both an academic and an industrial level.
26920320	7	5	theme	strong	1152:1157	arg1	impact					1159:1164	strong impact	1152:1164	strong impact at both an academic and an industrial level	1152:1208	Determining FOS composition directly from the FTIR spectra represents a useful tool to monitor enzymatic synthesis, with strong impact at both an academic and an industrial level.
26920320	0	6	from	Effect	0:5	arg1	composition					39:49	the composition	35:49	the composition of enzymatically synthesized short-chain fructo-oligosaccharides	35:114	Effect of sucrose concentration on the composition of enzymatically synthesized short-chain fructo-oligosaccharides as determined by FTIR and multivariate analysis.
26920320	2	7	theme	square	500:505	arg1	based-models					513:524	partial least square (PLS) based-models	486:524	partial least square (PLS) based-models	486:524	As their composition is determined by the synthesis conditions, the goals of this work were: (a) to engineer FOS of different composition by adjusting the sucrose concentration used as initial substrate; (b) to define partial least square (PLS) based-models to quantify all the sugars present in the reaction medium directly from the FTIR spectra.
26920320	3	8	theme	initial	679:685	arg1	sucrose					687:693	initial sucrose	679:693	initial sucrose	679:693	The yield of each reaction was calculated as the percentage of initial sucrose converted to each oligosaccharide, as monitored by HPLC.
26920320	2	9	theme	present	553:559	arg1	sugars					546:551	all the sugars	538:551	all the sugars present in the reaction medium directly from the FTIR spectra	538:613	As their composition is determined by the synthesis conditions, the goals of this work were: (a) to engineer FOS of different composition by adjusting the sucrose concentration used as initial substrate; (b) to define partial least square (PLS) based-models to quantify all the sugars present in the reaction medium directly from the FTIR spectra.
26920320	2	10	theme	synthesis	310:318	arg1	conditions					320:329	the synthesis conditions	306:329	the synthesis conditions	306:329	As their composition is determined by the synthesis conditions, the goals of this work were: (a) to engineer FOS of different composition by adjusting the sucrose concentration used as initial substrate; (b) to define partial least square (PLS) based-models to quantify all the sugars present in the reaction medium directly from the FTIR spectra.
26920320	3	11	theme	sucrose	687:693	arg1	percentage					665:674	the percentage	661:674	the percentage of initial sucrose	661:693	The yield of each reaction was calculated as the percentage of initial sucrose converted to each oligosaccharide, as monitored by HPLC.
26920320	2	12	theme	FTIR	602:605	arg1	spectra					607:613	the FTIR spectra	598:613	the FTIR spectra	598:613	As their composition is determined by the synthesis conditions, the goals of this work were: (a) to engineer FOS of different composition by adjusting the sucrose concentration used as initial substrate; (b) to define partial least square (PLS) based-models to quantify all the sugars present in the reaction medium directly from the FTIR spectra.
26920320	2	13	theme	different	384:392	arg1	composition					394:404	different composition	384:404	different composition	384:404	As their composition is determined by the synthesis conditions, the goals of this work were: (a) to engineer FOS of different composition by adjusting the sucrose concentration used as initial substrate; (b) to define partial least square (PLS) based-models to quantify all the sugars present in the reaction medium directly from the FTIR spectra.
26920320	2	14	theme	partial	486:492	arg1	based-models					513:524	partial least square (PLS) based-models	486:524	partial least square (PLS) based-models	486:524	As their composition is determined by the synthesis conditions, the goals of this work were: (a) to engineer FOS of different composition by adjusting the sucrose concentration used as initial substrate; (b) to define partial least square (PLS) based-models to quantify all the sugars present in the reaction medium directly from the FTIR spectra.
26920320	5	15	theme	different	806:814	arg1	models					820:825	Six different PLS models	802:825	Six different PLS models aiming to determine the concentration of each carbohydrate present in the reaction medium	802:915	Six different PLS models aiming to determine the concentration of each carbohydrate present in the reaction medium were calibrated and independently validated.
26920320	7	16	theme	enzymatic	1126:1134	arg1	synthesis					1136:1144	enzymatic synthesis	1126:1144	enzymatic synthesis	1126:1144	Determining FOS composition directly from the FTIR spectra represents a useful tool to monitor enzymatic synthesis, with strong impact at both an academic and an industrial level.
26920320	5	17	theme	present	886:892	arg1	concentration					851:863	the concentration	847:863	the concentration of each carbohydrate present in the reaction medium	847:915	Six different PLS models aiming to determine the concentration of each carbohydrate present in the reaction medium were calibrated and independently validated.
26920320	5	18	theme	PLS	816:818	arg1	models					820:825	Six different PLS models	802:825	Six different PLS models aiming to determine the concentration of each carbohydrate present in the reaction medium	802:915	Six different PLS models aiming to determine the concentration of each carbohydrate present in the reaction medium were calibrated and independently validated.
26920320	7	19	theme	FOS	1043:1045	arg1	composition					1047:1057	FOS composition	1043:1057	FOS composition	1043:1057	Determining FOS composition directly from the FTIR spectra represents a useful tool to monitor enzymatic synthesis, with strong impact at both an academic and an industrial level.
26920320	5	20	from	concentration	851:863	arg1	medium					910:915	the reaction medium	897:915	the reaction medium	897:915	Six different PLS models aiming to determine the concentration of each carbohydrate present in the reaction medium were calibrated and independently validated.
26920320	7	21	dep	academic	1177:1184	arg1	an					1174:1175	an	1174:1175	an	1174:1175	Determining FOS composition directly from the FTIR spectra represents a useful tool to monitor enzymatic synthesis, with strong impact at both an academic and an industrial level.
26920320	1	22	theme	oligosaccharides	211:226	arg1	mixtures					199:206	mixtures	199:206	mixtures of oligosaccharides composed of fructose and glucose units	199:265	Fructo-oligosaccharides (FOS) are mixtures of oligosaccharides composed of fructose and glucose units.
26920320	1	22	theme	oligosaccharides	211:226	arg1	Fructo-oligosaccharides					165:187	Fructo-oligosaccharides	165:187	Fructo-oligosaccharides (FOS)	165:193	Fructo-oligosaccharides (FOS) are mixtures of oligosaccharides composed of fructose and glucose units.
26920320	0	23	theme	concentration	18:30	arg1	Effect					0:5	Effect	0:5	Effect of sucrose concentration on the composition of enzymatically synthesized short-chain fructo-oligosaccharides	0:114	Effect of sucrose concentration on the composition of enzymatically synthesized short-chain fructo-oligosaccharides as determined by FTIR and multivariate analysis.
26920320	2	24	from	present	553:559	arg1	medium					577:582	the reaction medium	564:582	the reaction medium directly from the FTIR spectra	564:613	As their composition is determined by the synthesis conditions, the goals of this work were: (a) to engineer FOS of different composition by adjusting the sucrose concentration used as initial substrate; (b) to define partial least square (PLS) based-models to quantify all the sugars present in the reaction medium directly from the FTIR spectra.
26920320	0	25	theme	multivariate	142:153	arg1	analysis					155:162	multivariate analysis	142:162	multivariate analysis	142:162	Effect of sucrose concentration on the composition of enzymatically synthesized short-chain fructo-oligosaccharides as determined by FTIR and multivariate analysis.
26920320	7	26	from	level	1204:1208	arg1	impact					1159:1164	strong impact	1152:1164	strong impact at both an academic and an industrial level	1152:1208	Determining FOS composition directly from the FTIR spectra represents a useful tool to monitor enzymatic synthesis, with strong impact at both an academic and an industrial level.
26920320	2	27	theme	sucrose	423:429	arg1	concentration					431:443	the sucrose concentration	419:443	the sucrose concentration used as initial substrate	419:469	As their composition is determined by the synthesis conditions, the goals of this work were: (a) to engineer FOS of different composition by adjusting the sucrose concentration used as initial substrate; (b) to define partial least square (PLS) based-models to quantify all the sugars present in the reaction medium directly from the FTIR spectra.
26920320	5	28	theme	reaction	901:908	arg1	medium					910:915	the reaction medium	897:915	the reaction medium	897:915	Six different PLS models aiming to determine the concentration of each carbohydrate present in the reaction medium were calibrated and independently validated.
26920320	7	29	theme	useful	1103:1108	arg1	tool					1110:1113	a useful tool	1101:1113	a useful tool to monitor enzymatic synthesis, with strong impact at both an academic and an industrial level	1101:1208	Determining FOS composition directly from the FTIR spectra represents a useful tool to monitor enzymatic synthesis, with strong impact at both an academic and an industrial level.
26920320	2	30	dep	square	500:505	arg1	PLS					508:510	PLS	508:510	PLS	508:510	As their composition is determined by the synthesis conditions, the goals of this work were: (a) to engineer FOS of different composition by adjusting the sucrose concentration used as initial substrate; (b) to define partial least square (PLS) based-models to quantify all the sugars present in the reaction medium directly from the FTIR spectra.
26920320	2	31	theme	composition	394:404	arg1	FOS					377:379	FOS	377:379	FOS of different composition	377:404	As their composition is determined by the synthesis conditions, the goals of this work were: (a) to engineer FOS of different composition by adjusting the sucrose concentration used as initial substrate; (b) to define partial least square (PLS) based-models to quantify all the sugars present in the reaction medium directly from the FTIR spectra.
26920320	2	32	from	medium	577:582	arg1	present					553:559	present	553:559	present	553:559	As their composition is determined by the synthesis conditions, the goals of this work were: (a) to engineer FOS of different composition by adjusting the sucrose concentration used as initial substrate; (b) to define partial least square (PLS) based-models to quantify all the sugars present in the reaction medium directly from the FTIR spectra.
26920320	2	33	from	spectra	607:613	arg1	medium					577:582	the reaction medium	564:582	the reaction medium directly from the FTIR spectra	564:613	As their composition is determined by the synthesis conditions, the goals of this work were: (a) to engineer FOS of different composition by adjusting the sucrose concentration used as initial substrate; (b) to define partial least square (PLS) based-models to quantify all the sugars present in the reaction medium directly from the FTIR spectra.
26920320	2	34	theme	initial	453:459	arg1	substrate					461:469	initial substrate	453:469	initial substrate	453:469	As their composition is determined by the synthesis conditions, the goals of this work were: (a) to engineer FOS of different composition by adjusting the sucrose concentration used as initial substrate; (b) to define partial least square (PLS) based-models to quantify all the sugars present in the reaction medium directly from the FTIR spectra.
26920320	3	35	theme	reaction	634:641	arg1	yield					620:624	The yield	616:624	The yield of each reaction	616:641	The yield of each reaction was calculated as the percentage of initial sucrose converted to each oligosaccharide, as monitored by HPLC.
26920320	2	36	theme	work	350:353	arg1	goals					336:340	the goals	332:340	the goals of this work	332:353	As their composition is determined by the synthesis conditions, the goals of this work were: (a) to engineer FOS of different composition by adjusting the sucrose concentration used as initial substrate; (b) to define partial least square (PLS) based-models to quantify all the sugars present in the reaction medium directly from the FTIR spectra.
26920320	1	37	theme	glucose	253:259	arg1	units					261:265	glucose units	253:265	glucose units	253:265	Fructo-oligosaccharides (FOS) are mixtures of oligosaccharides composed of fructose and glucose units.
26920320	2	38	dep	engineer	368:375	arg1	a					362:362	a	362:362	a	362:362	As their composition is determined by the synthesis conditions, the goals of this work were: (a) to engineer FOS of different composition by adjusting the sucrose concentration used as initial substrate; (b) to define partial least square (PLS) based-models to quantify all the sugars present in the reaction medium directly from the FTIR spectra.
26920320	2	38	dep	engineer	368:375	arg1	b					473:473	b	473:473	b	473:473	As their composition is determined by the synthesis conditions, the goals of this work were: (a) to engineer FOS of different composition by adjusting the sucrose concentration used as initial substrate; (b) to define partial least square (PLS) based-models to quantify all the sugars present in the reaction medium directly from the FTIR spectra.
26920320	2	38	dep	engineer	368:375	arg1	define					479:484	define	479:484	to define partial least square (PLS) based-models to quantify all the sugars present in the reaction medium directly from the FTIR spectra	476:613	As their composition is determined by the synthesis conditions, the goals of this work were: (a) to engineer FOS of different composition by adjusting the sucrose concentration used as initial substrate; (b) to define partial least square (PLS) based-models to quantify all the sugars present in the reaction medium directly from the FTIR spectra.
26920320	5	39	theme	carbohydrate	873:884	arg1	present					886:892	each carbohydrate present	868:892	each carbohydrate present	868:892	Six different PLS models aiming to determine the concentration of each carbohydrate present in the reaction medium were calibrated and independently validated.
26920320	6	40	theme	predicted	975:983	arg1	values					985:990	predicted values	975:990	predicted values	975:990	The means of predicted values fitted well to those obtained by HPLC.
26920320	0	41	theme	synthesized	68:78	arg1	fructo-oligosaccharides					92:114	enzymatically synthesized short-chain fructo-oligosaccharides	54:114	enzymatically synthesized short-chain fructo-oligosaccharides	54:114	Effect of sucrose concentration on the composition of enzymatically synthesized short-chain fructo-oligosaccharides as determined by FTIR and multivariate analysis.
26920320	7	42	from	academic	1177:1184	arg1	impact					1159:1164	strong impact	1152:1164	strong impact at both an academic and an industrial level	1152:1208	Determining FOS composition directly from the FTIR spectra represents a useful tool to monitor enzymatic synthesis, with strong impact at both an academic and an industrial level.
26920320	7	43	theme	industrial	1193:1202	arg1	level					1204:1208	an industrial level	1190:1208	an industrial level	1190:1208	Determining FOS composition directly from the FTIR spectra represents a useful tool to monitor enzymatic synthesis, with strong impact at both an academic and an industrial level.
26920320	0	44	theme	sucrose	10:16	arg1	concentration					18:30	sucrose concentration	10:30	sucrose concentration	10:30	Effect of sucrose concentration on the composition of enzymatically synthesized short-chain fructo-oligosaccharides as determined by FTIR and multivariate analysis.
26920320	6	45	theme	values	985:990	arg1	means					966:970	The means	962:970	The means of predicted values	962:990	The means of predicted values fitted well to those obtained by HPLC.
27855893	6	0	theme	thermal	900:906	arg1	stability					908:916	good thermal stability	895:916	good thermal stability of the starch	895:930	Thermogravimetric analysis showed a weight loss of 85.81±0.52% and a decomposition temperature of 320.16±0.35°C, which indicated that there was good thermal stability of the starch.
27855893	5	1	theme	height	723:728	arg1	1.09±0.05					740:748	1.09±0.05	740:748	1.09±0.05	740:748	Initial, peak and finishing gelatinization temperatures for the starch were 71.51±0.07, 75.05±0.15, and 78.25±0.18°C, respectively; the gelatinization enthalpy was 3.86±0.02J/g, and the peak height index was 1.09±0.05.
27855893	5	1	theme	height	723:728	arg1	index					730:734	the peak height index	714:734	the peak height index	714:734	Initial, peak and finishing gelatinization temperatures for the starch were 71.51±0.07, 75.05±0.15, and 78.25±0.18°C, respectively; the gelatinization enthalpy was 3.86±0.02J/g, and the peak height index was 1.09±0.05.
27855893	3	2	theme	X-ray	329:333	arg1	diffraction					335:345	X-ray diffraction	329:345	X-ray diffraction (XRD) analysis	329:360	X-ray diffraction (XRD) analysis showed a type-C starch with a relative crystallinity of 23.31±2.41%.
27855893	3	2	theme	X-ray	329:333	arg1	XRD					348:350	XRD	348:350	XRD	348:350	X-ray diffraction (XRD) analysis showed a type-C starch with a relative crystallinity of 23.31±2.41%.
27855893	4	3	theme	5.6μm	493:497	arg1	size					511:514	average size	503:514	average size of 3.93±1.47μm	503:529	The starch granules are polyhedral, with a diameter of 2.8 to 5.6μm and average size of 3.93±1.47μm.
27855893	4	3	theme	5.6μm	493:497	arg1	diameter					474:481	a diameter	472:481	a diameter of 2.8 to 5.6μm	472:497	The starch granules are polyhedral, with a diameter of 2.8 to 5.6μm and average size of 3.93±1.47μm.
27855893	2	4	dep	%	287:287	arg1	proteins					289:296	proteins	289:296	1.34±0.11% proteins	278:296	This starch contains 44.47±1.86% amylose, 4.84±0.29% moisture, 0.88±0.21% ash, 1.34±0.11% proteins and 92.73±0.48% carbohydrates.
27855893	6	5	theme	320.16±0.35°C	849:861	arg1	loss					794:797	a weight loss	785:797	a weight loss of 85.81±0.52%	785:812	Thermogravimetric analysis showed a weight loss of 85.81±0.52% and a decomposition temperature of 320.16±0.35°C, which indicated that there was good thermal stability of the starch.
27855893	6	5	theme	320.16±0.35°C	849:861	arg1	temperature					834:844	a decomposition temperature	818:844	a decomposition temperature of 320.16±0.35°C, which indicated that there was good thermal stability of the starch	818:930	Thermogravimetric analysis showed a weight loss of 85.81±0.52% and a decomposition temperature of 320.16±0.35°C, which indicated that there was good thermal stability of the starch.
27855893	0	6	from	tubers	62:67	arg1	Properties					16:25	Physicochemical Properties	0:25	Physicochemical Properties of Starch from Dioscorea pyrifolia tubers	0:67	Physicochemical Properties of Starch from Dioscorea pyrifolia tubers.
27855893	7	7	theme	Fish	933:936	arg1	FET					955:957	FET	955:957	FET	955:957	Fish embryo toxicity (FET) showed that the starch was not toxic and that it was suitable for food and non-food industries.
27855893	7	7	theme	Fish	933:936	arg1	toxicity					945:952	Fish embryo toxicity	933:952	Fish embryo toxicity (FET)	933:958	Fish embryo toxicity (FET) showed that the starch was not toxic and that it was suitable for food and non-food industries.
27855893	5	8	theme	peak	718:721	arg1	1.09±0.05					740:748	1.09±0.05	740:748	1.09±0.05	740:748	Initial, peak and finishing gelatinization temperatures for the starch were 71.51±0.07, 75.05±0.15, and 78.25±0.18°C, respectively; the gelatinization enthalpy was 3.86±0.02J/g, and the peak height index was 1.09±0.05.
27855893	5	8	theme	peak	718:721	arg1	index					730:734	the peak height index	714:734	the peak height index	714:734	Initial, peak and finishing gelatinization temperatures for the starch were 71.51±0.07, 75.05±0.15, and 78.25±0.18°C, respectively; the gelatinization enthalpy was 3.86±0.02J/g, and the peak height index was 1.09±0.05.
27855893	6	9	theme	weight	787:792	arg1	loss					794:797	a weight loss	785:797	a weight loss of 85.81±0.52%	785:812	Thermogravimetric analysis showed a weight loss of 85.81±0.52% and a decomposition temperature of 320.16±0.35°C, which indicated that there was good thermal stability of the starch.
27855893	4	10	with	polyhedral	455:464	arg1	size					511:514	average size	503:514	average size of 3.93±1.47μm	503:529	The starch granules are polyhedral, with a diameter of 2.8 to 5.6μm and average size of 3.93±1.47μm.
27855893	4	10	with	polyhedral	455:464	arg1	diameter					474:481	a diameter	472:481	a diameter of 2.8 to 5.6μm	472:497	The starch granules are polyhedral, with a diameter of 2.8 to 5.6μm and average size of 3.93±1.47μm.
27855893	0	11	theme	pyrifolia	52:60	arg1	tubers					62:67	Dioscorea pyrifolia tubers	42:67	Dioscorea pyrifolia tubers	42:67	Physicochemical Properties of Starch from Dioscorea pyrifolia tubers.
27855893	5	12	theme	peak	541:544	arg1	temperatures					575:586	Initial, peak and finishing gelatinization temperatures	532:586	Initial, peak and finishing gelatinization temperatures for the starch	532:601	Initial, peak and finishing gelatinization temperatures for the starch were 71.51±0.07, 75.05±0.15, and 78.25±0.18°C, respectively; the gelatinization enthalpy was 3.86±0.02J/g, and the peak height index was 1.09±0.05.
27855893	1	13	theme	Dioscorea	82:90	arg1	tubers					102:107	Dioscorea pyrifolia tubers	82:107	Dioscorea pyrifolia tubers	82:107	Starch from Dioscorea pyrifolia tubers was characterized for its proximate composition, physicochemical properties and toxicity.
27855893	1	14	theme	pyrifolia	92:100	arg1	tubers					102:107	Dioscorea pyrifolia tubers	82:107	Dioscorea pyrifolia tubers	82:107	Starch from Dioscorea pyrifolia tubers was characterized for its proximate composition, physicochemical properties and toxicity.
27855893	0	15	theme	Physicochemical	0:14	arg1	Properties					16:25	Physicochemical Properties	0:25	Physicochemical Properties of Starch from Dioscorea pyrifolia tubers	0:67	Physicochemical Properties of Starch from Dioscorea pyrifolia tubers.
27855893	7	16	theme	non-food	1035:1042	arg1	industries					1044:1053	food and non-food industries	1026:1053	food and non-food industries	1026:1053	Fish embryo toxicity (FET) showed that the starch was not toxic and that it was suitable for food and non-food industries.
27855893	5	17	theme	finishing	550:558	arg1	temperatures					575:586	Initial, peak and finishing gelatinization temperatures	532:586	Initial, peak and finishing gelatinization temperatures for the starch	532:601	Initial, peak and finishing gelatinization temperatures for the starch were 71.51±0.07, 75.05±0.15, and 78.25±0.18°C, respectively; the gelatinization enthalpy was 3.86±0.02J/g, and the peak height index was 1.09±0.05.
27855893	3	18	theme	%	428:428	arg1	crystallinity					401:413	a relative crystallinity	390:413	a relative crystallinity of 23.31±2.41%	390:428	X-ray diffraction (XRD) analysis showed a type-C starch with a relative crystallinity of 23.31±2.41%.
27855893	0	19	theme	Starch	30:35	arg1	Properties					16:25	Physicochemical Properties	0:25	Physicochemical Properties of Starch from Dioscorea pyrifolia tubers	0:67	Physicochemical Properties of Starch from Dioscorea pyrifolia tubers.
27855893	4	20	theme	3.93±1.47μm	519:529	arg1	size					511:514	average size	503:514	average size of 3.93±1.47μm	503:529	The starch granules are polyhedral, with a diameter of 2.8 to 5.6μm and average size of 3.93±1.47μm.
27855893	4	20	theme	3.93±1.47μm	519:529	arg1	diameter					474:481	a diameter	472:481	a diameter of 2.8 to 5.6μm	472:497	The starch granules are polyhedral, with a diameter of 2.8 to 5.6μm and average size of 3.93±1.47μm.
27855893	5	21	theme	gelatinization	560:573	arg1	temperatures					575:586	Initial, peak and finishing gelatinization temperatures	532:586	Initial, peak and finishing gelatinization temperatures for the starch	532:601	Initial, peak and finishing gelatinization temperatures for the starch were 71.51±0.07, 75.05±0.15, and 78.25±0.18°C, respectively; the gelatinization enthalpy was 3.86±0.02J/g, and the peak height index was 1.09±0.05.
27855893	3	22	with	starch	378:383	arg1	crystallinity					401:413	a relative crystallinity	390:413	a relative crystallinity of 23.31±2.41%	390:428	X-ray diffraction (XRD) analysis showed a type-C starch with a relative crystallinity of 23.31±2.41%.
27855893	2	23	contain	contains	211:218	arg2	%					230:230	44.47±1.86%	220:230	44.47±1.86% amylose	220:238	This starch contains 44.47±1.86% amylose, 4.84±0.29% moisture, 0.88±0.21% ash, 1.34±0.11% proteins and 92.73±0.48% carbohydrates.
27855893	2	23	contain	contains	211:218	arg2	%					287:287	1.34±0.11%	278:287	1.34±0.11% proteins	278:296	This starch contains 44.47±1.86% amylose, 4.84±0.29% moisture, 0.88±0.21% ash, 1.34±0.11% proteins and 92.73±0.48% carbohydrates.
27855893	2	23	contain	contains	211:218	arg2	%					271:271	0.88±0.21%	262:271	0.88±0.21% ash	262:275	This starch contains 44.47±1.86% amylose, 4.84±0.29% moisture, 0.88±0.21% ash, 1.34±0.11% proteins and 92.73±0.48% carbohydrates.
27855893	2	23	contain	contains	211:218	arg2	%					312:312	92.73±0.48%	302:312	92.73±0.48% carbohydrates	302:326	This starch contains 44.47±1.86% amylose, 4.84±0.29% moisture, 0.88±0.21% ash, 1.34±0.11% proteins and 92.73±0.48% carbohydrates.
27855893	2	23	contain	contains	211:218	arg1	starch					204:209	This starch	199:209	This starch	199:209	This starch contains 44.47±1.86% amylose, 4.84±0.29% moisture, 0.88±0.21% ash, 1.34±0.11% proteins and 92.73±0.48% carbohydrates.
27855893	2	23	contain	contains	211:218	arg2	%					250:250	4.84±0.29%	241:250	4.84±0.29% moisture	241:259	This starch contains 44.47±1.86% amylose, 4.84±0.29% moisture, 0.88±0.21% ash, 1.34±0.11% proteins and 92.73±0.48% carbohydrates.
27855893	3	24	theme	type-C	371:376	arg1	starch					378:383	a type-C starch	369:383	a type-C starch with a relative crystallinity of 23.31±2.41%	369:428	X-ray diffraction (XRD) analysis showed a type-C starch with a relative crystallinity of 23.31±2.41%.
27855893	7	25	theme	food	1026:1029	arg1	industries					1044:1053	food and non-food industries	1026:1053	food and non-food industries	1026:1053	Fish embryo toxicity (FET) showed that the starch was not toxic and that it was suitable for food and non-food industries.
27855893	7	26	theme	embryo	938:943	arg1	FET					955:957	FET	955:957	FET	955:957	Fish embryo toxicity (FET) showed that the starch was not toxic and that it was suitable for food and non-food industries.
27855893	7	26	theme	embryo	938:943	arg1	toxicity					945:952	Fish embryo toxicity	933:952	Fish embryo toxicity (FET)	933:958	Fish embryo toxicity (FET) showed that the starch was not toxic and that it was suitable for food and non-food industries.
27855893	0	27	theme	Dioscorea	42:50	arg1	tubers					62:67	Dioscorea pyrifolia tubers	42:67	Dioscorea pyrifolia tubers	42:67	Physicochemical Properties of Starch from Dioscorea pyrifolia tubers.
27855893	4	28	theme	starch	435:440	arg1	granules					442:449	The starch granules	431:449	The starch granules	431:449	The starch granules are polyhedral, with a diameter of 2.8 to 5.6μm and average size of 3.93±1.47μm.
27855893	4	28	theme	starch	435:440	arg1	polyhedral					455:464	polyhedral	455:464	polyhedral	455:464	The starch granules are polyhedral, with a diameter of 2.8 to 5.6μm and average size of 3.93±1.47μm.
27855893	2	29	dep	%	271:271	arg1	ash					273:275	ash	273:275	0.88±0.21% ash	262:275	This starch contains 44.47±1.86% amylose, 4.84±0.29% moisture, 0.88±0.21% ash, 1.34±0.11% proteins and 92.73±0.48% carbohydrates.
27855893	4	30	theme	average	503:509	arg1	size					511:514	average size	503:514	average size of 3.93±1.47μm	503:529	The starch granules are polyhedral, with a diameter of 2.8 to 5.6μm and average size of 3.93±1.47μm.
27855893	2	31	dep	%	250:250	arg1	moisture					252:259	moisture	252:259	4.84±0.29% moisture	241:259	This starch contains 44.47±1.86% amylose, 4.84±0.29% moisture, 0.88±0.21% ash, 1.34±0.11% proteins and 92.73±0.48% carbohydrates.
27855893	4	32	dep	5.6μm	493:497	arg1	to					490:491	to	490:491	to	490:491	The starch granules are polyhedral, with a diameter of 2.8 to 5.6μm and average size of 3.93±1.47μm.
27855893	3	33	theme	relative	392:399	arg1	crystallinity					401:413	a relative crystallinity	390:413	a relative crystallinity of 23.31±2.41%	390:428	X-ray diffraction (XRD) analysis showed a type-C starch with a relative crystallinity of 23.31±2.41%.
27855893	6	34	theme	decomposition	820:832	arg1	temperature					834:844	a decomposition temperature	818:844	a decomposition temperature of 320.16±0.35°C, which indicated that there was good thermal stability of the starch	818:930	Thermogravimetric analysis showed a weight loss of 85.81±0.52% and a decomposition temperature of 320.16±0.35°C, which indicated that there was good thermal stability of the starch.
27855893	5	35	theme	Initial	532:538	arg1	temperatures					575:586	Initial, peak and finishing gelatinization temperatures	532:586	Initial, peak and finishing gelatinization temperatures for the starch	532:601	Initial, peak and finishing gelatinization temperatures for the starch were 71.51±0.07, 75.05±0.15, and 78.25±0.18°C, respectively; the gelatinization enthalpy was 3.86±0.02J/g, and the peak height index was 1.09±0.05.
27855893	6	36	theme	Thermogravimetric	751:767	arg1	analysis					769:776	Thermogravimetric analysis	751:776	Thermogravimetric analysis	751:776	Thermogravimetric analysis showed a weight loss of 85.81±0.52% and a decomposition temperature of 320.16±0.35°C, which indicated that there was good thermal stability of the starch.
27855893	1	37	theme	proximate	135:143	arg1	composition					145:155	its proximate composition	131:155	its proximate composition	131:155	Starch from Dioscorea pyrifolia tubers was characterized for its proximate composition, physicochemical properties and toxicity.
27855893	6	38	theme	good	895:898	arg1	stability					908:916	good thermal stability	895:916	good thermal stability of the starch	895:930	Thermogravimetric analysis showed a weight loss of 85.81±0.52% and a decomposition temperature of 320.16±0.35°C, which indicated that there was good thermal stability of the starch.
27855893	5	39	theme	gelatinization	668:681	arg1	3.86±0.02J/g					696:707	3.86±0.02J/g	696:707	3.86±0.02J/g	696:707	Initial, peak and finishing gelatinization temperatures for the starch were 71.51±0.07, 75.05±0.15, and 78.25±0.18°C, respectively; the gelatinization enthalpy was 3.86±0.02J/g, and the peak height index was 1.09±0.05.
27855893	5	39	theme	gelatinization	668:681	arg1	enthalpy					683:690	the gelatinization enthalpy	664:690	the gelatinization enthalpy	664:690	Initial, peak and finishing gelatinization temperatures for the starch were 71.51±0.07, 75.05±0.15, and 78.25±0.18°C, respectively; the gelatinization enthalpy was 3.86±0.02J/g, and the peak height index was 1.09±0.05.
27855893	2	40	dep	%	312:312	arg1	carbohydrates					314:326	carbohydrates	314:326	92.73±0.48% carbohydrates	302:326	This starch contains 44.47±1.86% amylose, 4.84±0.29% moisture, 0.88±0.21% ash, 1.34±0.11% proteins and 92.73±0.48% carbohydrates.
27855893	3	41	theme	diffraction	335:345	arg1	analysis					353:360	X-ray diffraction (XRD) analysis	329:360	X-ray diffraction (XRD) analysis	329:360	X-ray diffraction (XRD) analysis showed a type-C starch with a relative crystallinity of 23.31±2.41%.
27855893	6	42	theme	%	812:812	arg1	loss					794:797	a weight loss	785:797	a weight loss of 85.81±0.52%	785:812	Thermogravimetric analysis showed a weight loss of 85.81±0.52% and a decomposition temperature of 320.16±0.35°C, which indicated that there was good thermal stability of the starch.
27855893	6	42	theme	%	812:812	arg1	temperature					834:844	a decomposition temperature	818:844	a decomposition temperature of 320.16±0.35°C, which indicated that there was good thermal stability of the starch	818:930	Thermogravimetric analysis showed a weight loss of 85.81±0.52% and a decomposition temperature of 320.16±0.35°C, which indicated that there was good thermal stability of the starch.
27855893	1	43	from	tubers	102:107	arg1	Starch					70:75	Starch	70:75	Starch from Dioscorea pyrifolia tubers	70:107	Starch from Dioscorea pyrifolia tubers was characterized for its proximate composition, physicochemical properties and toxicity.
27855893	1	44	theme	physicochemical	158:172	arg1	properties					174:183	physicochemical properties	158:183	physicochemical properties	158:183	Starch from Dioscorea pyrifolia tubers was characterized for its proximate composition, physicochemical properties and toxicity.
27855893	6	45	theme	starch	925:930	arg1	stability					908:916	good thermal stability	895:916	good thermal stability of the starch	895:930	Thermogravimetric analysis showed a weight loss of 85.81±0.52% and a decomposition temperature of 320.16±0.35°C, which indicated that there was good thermal stability of the starch.
27855893	2	46	dep	%	230:230	arg1	amylose					232:238	amylose	232:238	44.47±1.86% amylose	220:238	This starch contains 44.47±1.86% amylose, 4.84±0.29% moisture, 0.88±0.21% ash, 1.34±0.11% proteins and 92.73±0.48% carbohydrates.
27425182	4	0	theme	smooth	608:613	arg1	fibroblasts					629:639	fibroblasts	629:639	fibroblasts	629:639	METHODS Collagen-chitosan composite material was used as the cell matrix, and smooth muscle cells, fibroblasts and BMSCs were used as seed cells.
27425182	4	0	theme	smooth	608:613	arg1	cells					622:626	smooth muscle cells	608:626	smooth muscle cells	608:626	METHODS Collagen-chitosan composite material was used as the cell matrix, and smooth muscle cells, fibroblasts and BMSCs were used as seed cells.
27425182	4	0	theme	smooth	608:613	arg1	cells					669:673	seed cells	664:673	seed cells	664:673	METHODS Collagen-chitosan composite material was used as the cell matrix, and smooth muscle cells, fibroblasts and BMSCs were used as seed cells.
27425182	4	0	theme	smooth	608:613	arg1	BMSCs					645:649	BMSCs	645:649	BMSCs	645:649	METHODS Collagen-chitosan composite material was used as the cell matrix, and smooth muscle cells, fibroblasts and BMSCs were used as seed cells.
27425182	2	1	theme	mesenchymal	384:394	arg1	BMSCs					408:412	BMSCs	408:412	BMSCs	408:412	The aims of this study were to demonstrate that the collagen-chitosan composite scaffolds are cytocompatible, and endothelial cells can be differentiated from bone marrow mesenchymal stem cells (BMSCs) when seeded onto the scaffolds.
27425182	2	1	theme	mesenchymal	384:394	arg1	cells					401:405	mesenchymal stem cells	384:405	bone marrow mesenchymal stem cells (BMSCs)	372:413	The aims of this study were to demonstrate that the collagen-chitosan composite scaffolds are cytocompatible, and endothelial cells can be differentiated from bone marrow mesenchymal stem cells (BMSCs) when seeded onto the scaffolds.
27425182	7	2	theme	smooth	1136:1141	arg1	actin					1150:1154	smooth muscle actin	1136:1154	smooth muscle actin	1136:1154	RESULTS The tissue engineered heart valves stained positively for both smooth muscle actin and endothelial cell factor VIII, suggesting that the seeded cells were in fact smooth muscle cells, fibroblasts, and endothelial cells.
27425182	7	3	dep	RESULTS	1065:1071	arg1	valves					1101:1106	The tissue engineered heart valves	1073:1106	RESULTS The tissue engineered heart valves	1065:1106	RESULTS The tissue engineered heart valves stained positively for both smooth muscle actin and endothelial cell factor VIII, suggesting that the seeded cells were in fact smooth muscle cells, fibroblasts, and endothelial cells.
27425182	0	4	theme	Degradable	0:9	arg1	Composites					29:38	Degradable Chitosan-Collagen Composites	0:38	Degradable Chitosan-Collagen Composites	0:38	Degradable Chitosan-Collagen Composites Seeded with Cells as Tissue Engineered Heart Valves.
27425182	1	5	used	used	163:166	arg2	materials					143:151	BACKGROUND Degradable collagen-chitosan composite materials	93:151	BACKGROUND Degradable collagen-chitosan composite materials	93:151	BACKGROUND Degradable collagen-chitosan composite materials have been used to fabricate tissue engineered heart valves.
27425182	1	6	theme	Degradable	104:113	arg1	materials					143:151	BACKGROUND Degradable collagen-chitosan composite materials	93:151	BACKGROUND Degradable collagen-chitosan composite materials	93:151	BACKGROUND Degradable collagen-chitosan composite materials have been used to fabricate tissue engineered heart valves.
27425182	7	7	theme	heart	1095:1099	arg1	valves					1101:1106	The tissue engineered heart valves	1073:1106	RESULTS The tissue engineered heart valves	1065:1106	RESULTS The tissue engineered heart valves stained positively for both smooth muscle actin and endothelial cell factor VIII, suggesting that the seeded cells were in fact smooth muscle cells, fibroblasts, and endothelial cells.
27425182	2	8	theme	endothelial	327:337	arg1	cells					339:343	endothelial cells	327:343	endothelial cells	327:343	The aims of this study were to demonstrate that the collagen-chitosan composite scaffolds are cytocompatible, and endothelial cells can be differentiated from bone marrow mesenchymal stem cells (BMSCs) when seeded onto the scaffolds.
27425182	6	9	theme	valve	954:958	arg1	scaffolds					960:968	heart valve scaffolds	948:968	heart valve scaffolds	948:968	After four weeks in culture, the cellular density and activity were assessed on segments of the tissue engineered heart valve scaffolds to determine the cell viability and proliferation in the collagen-chitosan composite material.
27425182	4	10	theme	seed	664:667	arg1	fibroblasts					629:639	fibroblasts	629:639	fibroblasts	629:639	METHODS Collagen-chitosan composite material was used as the cell matrix, and smooth muscle cells, fibroblasts and BMSCs were used as seed cells.
27425182	4	10	theme	seed	664:667	arg1	cells					622:626	smooth muscle cells	608:626	smooth muscle cells	608:626	METHODS Collagen-chitosan composite material was used as the cell matrix, and smooth muscle cells, fibroblasts and BMSCs were used as seed cells.
27425182	4	10	theme	seed	664:667	arg1	cells					669:673	seed cells	664:673	seed cells	664:673	METHODS Collagen-chitosan composite material was used as the cell matrix, and smooth muscle cells, fibroblasts and BMSCs were used as seed cells.
27425182	4	10	theme	seed	664:667	arg1	BMSCs					645:649	BMSCs	645:649	BMSCs	645:649	METHODS Collagen-chitosan composite material was used as the cell matrix, and smooth muscle cells, fibroblasts and BMSCs were used as seed cells.
27425182	5	11	theme	smooth	718:723	arg1	cells					732:736	the smooth muscle cells	714:736	the smooth muscle cells	714:736	After four weeks of in vitro culture, the smooth muscle cells, fibroblasts, and BMSCs were sequentially seeded into the collagen-chitosan composite material.
27425182	9	12	theme	electron	1473:1480	arg1	microscopy					1482:1491	electron microscopy	1473:1491	electron microscopy	1473:1491	Light and electron microscopy showed that the seeded cells had shapes similar to the morphology of smooth muscle cells, fibroblasts, and endothelial cells.
27425182	8	13	theme	collagen-chitosan	1369:1385	arg1	fluid					1400:1404	the collagen-chitosan cell culture fluid	1365:1404	the collagen-chitosan cell culture fluid	1365:1404	The 6-ketone prostaglandin content, as measured by radioimmunoassay, of the collagen-chitosan cell culture fluid was higher than that of the serum-free medium (P <0.01).
27425182	8	14	theme	6-ketone	1297:1304	arg1	content					1320:1326	The 6-ketone prostaglandin content	1293:1326	The 6-ketone prostaglandin content, as measured by radioimmunoassay, of the collagen-chitosan cell culture fluid	1293:1404	The 6-ketone prostaglandin content, as measured by radioimmunoassay, of the collagen-chitosan cell culture fluid was higher than that of the serum-free medium (P <0.01).
27425182	8	14	theme	6-ketone	1297:1304	arg1	higher					1410:1415	higher	1410:1415	higher	1410:1415	The 6-ketone prostaglandin content, as measured by radioimmunoassay, of the collagen-chitosan cell culture fluid was higher than that of the serum-free medium (P <0.01).
27425182	6	15	from	weeks	845:849	arg1	culture					854:860	culture	854:860	culture	854:860	After four weeks in culture, the cellular density and activity were assessed on segments of the tissue engineered heart valve scaffolds to determine the cell viability and proliferation in the collagen-chitosan composite material.
27425182	1	16	theme	collagen-chitosan	115:131	arg1	materials					143:151	BACKGROUND Degradable collagen-chitosan composite materials	93:151	BACKGROUND Degradable collagen-chitosan composite materials	93:151	BACKGROUND Degradable collagen-chitosan composite materials have been used to fabricate tissue engineered heart valves.
27425182	2	17	theme	composite	283:291	arg1	cytocompatible					307:320	cytocompatible	307:320	cytocompatible	307:320	The aims of this study were to demonstrate that the collagen-chitosan composite scaffolds are cytocompatible, and endothelial cells can be differentiated from bone marrow mesenchymal stem cells (BMSCs) when seeded onto the scaffolds.
27425182	2	17	theme	composite	283:291	arg1	scaffolds					293:301	the collagen-chitosan composite scaffolds	261:301	the collagen-chitosan composite scaffolds	261:301	The aims of this study were to demonstrate that the collagen-chitosan composite scaffolds are cytocompatible, and endothelial cells can be differentiated from bone marrow mesenchymal stem cells (BMSCs) when seeded onto the scaffolds.
27425182	11	18	theme	seeded	1746:1751	arg1	cells					1753:1757	The seeded cells	1742:1757	The seeded cells	1742:1757	The seeded cells retained their biological activity after being cultured in vitro and seeded into the collagen-chitosan composite material.
27425182	7	19	theme	muscle	1243:1248	arg1	cells					1250:1254	fact smooth muscle cells	1231:1254	fact smooth muscle cells	1231:1254	RESULTS The tissue engineered heart valves stained positively for both smooth muscle actin and endothelial cell factor VIII, suggesting that the seeded cells were in fact smooth muscle cells, fibroblasts, and endothelial cells.
27425182	7	20	theme	fact	1231:1234	arg1	cells					1250:1254	fact smooth muscle cells	1231:1254	fact smooth muscle cells	1231:1254	RESULTS The tissue engineered heart valves stained positively for both smooth muscle actin and endothelial cell factor VIII, suggesting that the seeded cells were in fact smooth muscle cells, fibroblasts, and endothelial cells.
27425182	1	21	theme	engineered	188:197	arg1	valves					205:210	tissue engineered heart valves	181:210	tissue engineered heart valves	181:210	BACKGROUND Degradable collagen-chitosan composite materials have been used to fabricate tissue engineered heart valves.
27425182	5	22	dep	in	696:697	arg1	vitro					699:703	vitro	699:703	vitro	699:703	After four weeks of in vitro culture, the smooth muscle cells, fibroblasts, and BMSCs were sequentially seeded into the collagen-chitosan composite material.
27425182	4	23	theme	Collagen-chitosan	538:554	arg1	material					566:573	METHODS Collagen-chitosan composite material	530:573	METHODS Collagen-chitosan composite material	530:573	METHODS Collagen-chitosan composite material was used as the cell matrix, and smooth muscle cells, fibroblasts and BMSCs were used as seed cells.
27425182	4	23	theme	Collagen-chitosan	538:554	arg1	matrix					596:601	the cell matrix	587:601	the cell matrix	587:601	METHODS Collagen-chitosan composite material was used as the cell matrix, and smooth muscle cells, fibroblasts and BMSCs were used as seed cells.
27425182	10	24	theme	composite	1721:1729	arg1	scaffolds					1731:1739	the collagen-chitosan composite scaffolds	1699:1739	the collagen-chitosan composite scaffolds	1699:1739	CONCLUSIONS Endothelial cells can be differentiated from BMSCs when seeded onto the collagen-chitosan composite scaffolds.
27425182	0	25	dep	Engineered	68:77	arg1	Tissue					61:66	Tissue	61:66	Tissue	61:66	Degradable Chitosan-Collagen Composites Seeded with Cells as Tissue Engineered Heart Valves.
27425182	11	26	theme	biological	1774:1783	arg1	activity					1785:1792	their biological activity	1768:1792	their biological activity	1768:1792	The seeded cells retained their biological activity after being cultured in vitro and seeded into the collagen-chitosan composite material.
27425182	7	27	theme	seeded	1210:1215	arg1	cells					1217:1221	the seeded cells	1206:1221	the seeded cells	1206:1221	RESULTS The tissue engineered heart valves stained positively for both smooth muscle actin and endothelial cell factor VIII, suggesting that the seeded cells were in fact smooth muscle cells, fibroblasts, and endothelial cells.
27425182	0	28	theme	Heart	79:83	arg1	Valves					85:90	Tissue Engineered Heart Valves	61:90	Tissue Engineered Heart Valves	61:90	Degradable Chitosan-Collagen Composites Seeded with Cells as Tissue Engineered Heart Valves.
27425182	8	29	theme	cell	1387:1390	arg1	fluid					1400:1404	the collagen-chitosan cell culture fluid	1365:1404	the collagen-chitosan cell culture fluid	1365:1404	The 6-ketone prostaglandin content, as measured by radioimmunoassay, of the collagen-chitosan cell culture fluid was higher than that of the serum-free medium (P <0.01).
27425182	5	30	theme	composite	814:822	arg1	material					824:831	the collagen-chitosan composite material	792:831	the collagen-chitosan composite material	792:831	After four weeks of in vitro culture, the smooth muscle cells, fibroblasts, and BMSCs were sequentially seeded into the collagen-chitosan composite material.
27425182	9	31	theme	smooth	1562:1567	arg1	cells					1576:1580	smooth muscle cells	1562:1580	smooth muscle cells	1562:1580	Light and electron microscopy showed that the seeded cells had shapes similar to the morphology of smooth muscle cells, fibroblasts, and endothelial cells.
27425182	2	32	dep	marrow	377:382	arg1	BMSCs					408:412	BMSCs	408:412	BMSCs	408:412	The aims of this study were to demonstrate that the collagen-chitosan composite scaffolds are cytocompatible, and endothelial cells can be differentiated from bone marrow mesenchymal stem cells (BMSCs) when seeded onto the scaffolds.
27425182	2	32	dep	marrow	377:382	arg1	cells					401:405	mesenchymal stem cells	384:405	bone marrow mesenchymal stem cells (BMSCs)	372:413	The aims of this study were to demonstrate that the collagen-chitosan composite scaffolds are cytocompatible, and endothelial cells can be differentiated from bone marrow mesenchymal stem cells (BMSCs) when seeded onto the scaffolds.
27425182	6	33	theme	cellular	867:874	arg1	density					876:882	the cellular density	863:882	the cellular density	863:882	After four weeks in culture, the cellular density and activity were assessed on segments of the tissue engineered heart valve scaffolds to determine the cell viability and proliferation in the collagen-chitosan composite material.
27425182	9	34	theme	cells	1576:1580	arg1	morphology					1548:1557	the morphology	1544:1557	the morphology of smooth muscle cells, fibroblasts, and endothelial cells	1544:1616	Light and electron microscopy showed that the seeded cells had shapes similar to the morphology of smooth muscle cells, fibroblasts, and endothelial cells.
27425182	7	35	theme	factor	1177:1182	arg1	VIII					1184:1187	endothelial cell factor VIII	1160:1187	endothelial cell factor VIII	1160:1187	RESULTS The tissue engineered heart valves stained positively for both smooth muscle actin and endothelial cell factor VIII, suggesting that the seeded cells were in fact smooth muscle cells, fibroblasts, and endothelial cells.
27425182	3	36	dep	adhesion	451:458	arg1	The					447:449	The	447:449	The	447:449	The adhesion and biological activities of the seeded cells were also investigated.
27425182	6	37	theme	composite	1045:1053	arg1	material					1055:1062	the collagen-chitosan composite material	1023:1062	the collagen-chitosan composite material	1023:1062	After four weeks in culture, the cellular density and activity were assessed on segments of the tissue engineered heart valve scaffolds to determine the cell viability and proliferation in the collagen-chitosan composite material.
27425182	0	38	theme	Engineered	68:77	arg1	Valves					85:90	Tissue Engineered Heart Valves	61:90	Tissue Engineered Heart Valves	61:90	Degradable Chitosan-Collagen Composites Seeded with Cells as Tissue Engineered Heart Valves.
27425182	10	39	theme	collagen-chitosan	1703:1719	arg1	scaffolds					1731:1739	the collagen-chitosan composite scaffolds	1699:1739	the collagen-chitosan composite scaffolds	1699:1739	CONCLUSIONS Endothelial cells can be differentiated from BMSCs when seeded onto the collagen-chitosan composite scaffolds.
27425182	7	40	theme	endothelial	1160:1170	arg1	VIII					1184:1187	endothelial cell factor VIII	1160:1187	endothelial cell factor VIII	1160:1187	RESULTS The tissue engineered heart valves stained positively for both smooth muscle actin and endothelial cell factor VIII, suggesting that the seeded cells were in fact smooth muscle cells, fibroblasts, and endothelial cells.
27425182	9	41	theme	fibroblasts	1583:1593	arg1	morphology					1548:1557	the morphology	1544:1557	the morphology of smooth muscle cells, fibroblasts, and endothelial cells	1544:1616	Light and electron microscopy showed that the seeded cells had shapes similar to the morphology of smooth muscle cells, fibroblasts, and endothelial cells.
27425182	3	42	theme	cells	500:504	arg1	adhesion					451:458	adhesion	451:458	adhesion	451:458	The adhesion and biological activities of the seeded cells were also investigated.
27425182	3	42	theme	cells	500:504	arg1	activities					475:484	biological activities	464:484	biological activities	464:484	The adhesion and biological activities of the seeded cells were also investigated.
27425182	9	43	theme	cells	1612:1616	arg1	morphology					1548:1557	the morphology	1544:1557	the morphology of smooth muscle cells, fibroblasts, and endothelial cells	1544:1616	Light and electron microscopy showed that the seeded cells had shapes similar to the morphology of smooth muscle cells, fibroblasts, and endothelial cells.
27425182	7	44	theme	endothelial	1274:1284	arg1	cells					1286:1290	endothelial cells	1274:1290	endothelial cells	1274:1290	RESULTS The tissue engineered heart valves stained positively for both smooth muscle actin and endothelial cell factor VIII, suggesting that the seeded cells were in fact smooth muscle cells, fibroblasts, and endothelial cells.
27425182	8	45	theme	fluid	1400:1404	arg1	content					1320:1326	The 6-ketone prostaglandin content	1293:1326	The 6-ketone prostaglandin content, as measured by radioimmunoassay, of the collagen-chitosan cell culture fluid	1293:1404	The 6-ketone prostaglandin content, as measured by radioimmunoassay, of the collagen-chitosan cell culture fluid was higher than that of the serum-free medium (P <0.01).
27425182	8	45	theme	fluid	1400:1404	arg1	higher					1410:1415	higher	1410:1415	higher	1410:1415	The 6-ketone prostaglandin content, as measured by radioimmunoassay, of the collagen-chitosan cell culture fluid was higher than that of the serum-free medium (P <0.01).
27425182	7	46	theme	muscle	1143:1148	arg1	actin					1150:1154	smooth muscle actin	1136:1154	smooth muscle actin	1136:1154	RESULTS The tissue engineered heart valves stained positively for both smooth muscle actin and endothelial cell factor VIII, suggesting that the seeded cells were in fact smooth muscle cells, fibroblasts, and endothelial cells.
27425182	2	47	theme	stem	396:399	arg1	BMSCs					408:412	BMSCs	408:412	BMSCs	408:412	The aims of this study were to demonstrate that the collagen-chitosan composite scaffolds are cytocompatible, and endothelial cells can be differentiated from bone marrow mesenchymal stem cells (BMSCs) when seeded onto the scaffolds.
27425182	2	47	theme	stem	396:399	arg1	cells					401:405	mesenchymal stem cells	384:405	bone marrow mesenchymal stem cells (BMSCs)	372:413	The aims of this study were to demonstrate that the collagen-chitosan composite scaffolds are cytocompatible, and endothelial cells can be differentiated from bone marrow mesenchymal stem cells (BMSCs) when seeded onto the scaffolds.
27425182	10	48	dep	CONCLUSIONS	1619:1629	arg1	differentiated					1656:1669	differentiated	1656:1669	can be differentiated from BMSCs when seeded onto the collagen-chitosan composite scaffolds	1649:1739	CONCLUSIONS Endothelial cells can be differentiated from BMSCs when seeded onto the collagen-chitosan composite scaffolds.
27425182	4	49	theme	muscle	615:620	arg1	fibroblasts					629:639	fibroblasts	629:639	fibroblasts	629:639	METHODS Collagen-chitosan composite material was used as the cell matrix, and smooth muscle cells, fibroblasts and BMSCs were used as seed cells.
27425182	4	49	theme	muscle	615:620	arg1	cells					622:626	smooth muscle cells	608:626	smooth muscle cells	608:626	METHODS Collagen-chitosan composite material was used as the cell matrix, and smooth muscle cells, fibroblasts and BMSCs were used as seed cells.
27425182	4	49	theme	muscle	615:620	arg1	cells					669:673	seed cells	664:673	seed cells	664:673	METHODS Collagen-chitosan composite material was used as the cell matrix, and smooth muscle cells, fibroblasts and BMSCs were used as seed cells.
27425182	4	49	theme	muscle	615:620	arg1	BMSCs					645:649	BMSCs	645:649	BMSCs	645:649	METHODS Collagen-chitosan composite material was used as the cell matrix, and smooth muscle cells, fibroblasts and BMSCs were used as seed cells.
27425182	4	50	used	used	579:582	arg2	material					566:573	METHODS Collagen-chitosan composite material	530:573	METHODS Collagen-chitosan composite material	530:573	METHODS Collagen-chitosan composite material was used as the cell matrix, and smooth muscle cells, fibroblasts and BMSCs were used as seed cells.
27425182	4	50	used	used	579:582	arg2	matrix					596:601	the cell matrix	587:601	the cell matrix	587:601	METHODS Collagen-chitosan composite material was used as the cell matrix, and smooth muscle cells, fibroblasts and BMSCs were used as seed cells.
27425182	1	51	theme	heart	199:203	arg1	valves					205:210	tissue engineered heart valves	181:210	tissue engineered heart valves	181:210	BACKGROUND Degradable collagen-chitosan composite materials have been used to fabricate tissue engineered heart valves.
27425182	1	52	theme	composite	133:141	arg1	materials					143:151	BACKGROUND Degradable collagen-chitosan composite materials	93:151	BACKGROUND Degradable collagen-chitosan composite materials	93:151	BACKGROUND Degradable collagen-chitosan composite materials have been used to fabricate tissue engineered heart valves.
27425182	5	53	theme	culture	705:711	arg1	weeks					687:691	four weeks	682:691	four weeks of in vitro culture	682:711	After four weeks of in vitro culture, the smooth muscle cells, fibroblasts, and BMSCs were sequentially seeded into the collagen-chitosan composite material.
27425182	8	54	theme	P	1453:1453	arg1	<0.01					1455:1459	P <0.01	1453:1459	P <0.01	1453:1459	The 6-ketone prostaglandin content, as measured by radioimmunoassay, of the collagen-chitosan cell culture fluid was higher than that of the serum-free medium (P <0.01).
27425182	8	54	theme	P	1453:1453	arg1	medium					1445:1450	the serum-free medium	1430:1450	the serum-free medium (P <0.01)	1430:1460	The 6-ketone prostaglandin content, as measured by radioimmunoassay, of the collagen-chitosan cell culture fluid was higher than that of the serum-free medium (P <0.01).
27425182	6	55	theme	heart	948:952	arg1	scaffolds					960:968	heart valve scaffolds	948:968	heart valve scaffolds	948:968	After four weeks in culture, the cellular density and activity were assessed on segments of the tissue engineered heart valve scaffolds to determine the cell viability and proliferation in the collagen-chitosan composite material.
27425182	11	56	theme	collagen-chitosan	1844:1860	arg1	material					1872:1879	the collagen-chitosan composite material	1840:1879	the collagen-chitosan composite material	1840:1879	The seeded cells retained their biological activity after being cultured in vitro and seeded into the collagen-chitosan composite material.
27425182	6	57	dep	viability	992:1000	arg1	the					983:985	the	983:985	the	983:985	After four weeks in culture, the cellular density and activity were assessed on segments of the tissue engineered heart valve scaffolds to determine the cell viability and proliferation in the collagen-chitosan composite material.
27425182	6	58	theme	tissue	930:935	arg1	segments					914:921	segments	914:921	segments of the tissue engineered heart valve scaffolds to determine the cell viability and proliferation in the collagen-chitosan composite material	914:1062	After four weeks in culture, the cellular density and activity were assessed on segments of the tissue engineered heart valve scaffolds to determine the cell viability and proliferation in the collagen-chitosan composite material.
27425182	7	59	theme	engineered	1084:1093	arg1	valves					1101:1106	The tissue engineered heart valves	1073:1106	RESULTS The tissue engineered heart valves	1065:1106	RESULTS The tissue engineered heart valves stained positively for both smooth muscle actin and endothelial cell factor VIII, suggesting that the seeded cells were in fact smooth muscle cells, fibroblasts, and endothelial cells.
27425182	5	60	theme	in	696:697	arg1	culture					705:711	in vitro culture	696:711	in vitro culture	696:711	After four weeks of in vitro culture, the smooth muscle cells, fibroblasts, and BMSCs were sequentially seeded into the collagen-chitosan composite material.
27425182	8	61	dep	content	1320:1326	arg1	measured					1332:1339	measured	1332:1339	measured by radioimmunoassay	1332:1359	The 6-ketone prostaglandin content, as measured by radioimmunoassay, of the collagen-chitosan cell culture fluid was higher than that of the serum-free medium (P <0.01).
27425182	6	62	theme	collagen-chitosan	1027:1043	arg1	material					1055:1062	the collagen-chitosan composite material	1023:1062	the collagen-chitosan composite material	1023:1062	After four weeks in culture, the cellular density and activity were assessed on segments of the tissue engineered heart valve scaffolds to determine the cell viability and proliferation in the collagen-chitosan composite material.
27425182	8	63	theme	prostaglandin	1306:1318	arg1	content					1320:1326	The 6-ketone prostaglandin content	1293:1326	The 6-ketone prostaglandin content, as measured by radioimmunoassay, of the collagen-chitosan cell culture fluid	1293:1404	The 6-ketone prostaglandin content, as measured by radioimmunoassay, of the collagen-chitosan cell culture fluid was higher than that of the serum-free medium (P <0.01).
27425182	8	63	theme	prostaglandin	1306:1318	arg1	higher					1410:1415	higher	1410:1415	higher	1410:1415	The 6-ketone prostaglandin content, as measured by radioimmunoassay, of the collagen-chitosan cell culture fluid was higher than that of the serum-free medium (P <0.01).
27425182	11	64	theme	composite	1862:1870	arg1	material					1872:1879	the collagen-chitosan composite material	1840:1879	the collagen-chitosan composite material	1840:1879	The seeded cells retained their biological activity after being cultured in vitro and seeded into the collagen-chitosan composite material.
27425182	7	65	theme	smooth	1236:1241	arg1	cells					1250:1254	fact smooth muscle cells	1231:1254	fact smooth muscle cells	1231:1254	RESULTS The tissue engineered heart valves stained positively for both smooth muscle actin and endothelial cell factor VIII, suggesting that the seeded cells were in fact smooth muscle cells, fibroblasts, and endothelial cells.
27425182	2	66	theme	collagen-chitosan	265:281	arg1	cytocompatible					307:320	cytocompatible	307:320	cytocompatible	307:320	The aims of this study were to demonstrate that the collagen-chitosan composite scaffolds are cytocompatible, and endothelial cells can be differentiated from bone marrow mesenchymal stem cells (BMSCs) when seeded onto the scaffolds.
27425182	2	66	theme	collagen-chitosan	265:281	arg1	scaffolds					293:301	the collagen-chitosan composite scaffolds	261:301	the collagen-chitosan composite scaffolds	261:301	The aims of this study were to demonstrate that the collagen-chitosan composite scaffolds are cytocompatible, and endothelial cells can be differentiated from bone marrow mesenchymal stem cells (BMSCs) when seeded onto the scaffolds.
27425182	9	67	theme	seeded	1509:1514	arg1	cells					1516:1520	the seeded cells	1505:1520	the seeded cells	1505:1520	Light and electron microscopy showed that the seeded cells had shapes similar to the morphology of smooth muscle cells, fibroblasts, and endothelial cells.
27425182	4	68	theme	METHODS	530:536	arg1	material					566:573	METHODS Collagen-chitosan composite material	530:573	METHODS Collagen-chitosan composite material	530:573	METHODS Collagen-chitosan composite material was used as the cell matrix, and smooth muscle cells, fibroblasts and BMSCs were used as seed cells.
27425182	4	68	theme	METHODS	530:536	arg1	matrix					596:601	the cell matrix	587:601	the cell matrix	587:601	METHODS Collagen-chitosan composite material was used as the cell matrix, and smooth muscle cells, fibroblasts and BMSCs were used as seed cells.
27425182	4	69	used	used	656:659	arg2	BMSCs					645:649	BMSCs	645:649	BMSCs	645:649	METHODS Collagen-chitosan composite material was used as the cell matrix, and smooth muscle cells, fibroblasts and BMSCs were used as seed cells.
27425182	4	69	used	used	656:659	arg2	fibroblasts					629:639	fibroblasts	629:639	fibroblasts	629:639	METHODS Collagen-chitosan composite material was used as the cell matrix, and smooth muscle cells, fibroblasts and BMSCs were used as seed cells.
27425182	4	69	used	used	656:659	arg2	cells					622:626	smooth muscle cells	608:626	smooth muscle cells	608:626	METHODS Collagen-chitosan composite material was used as the cell matrix, and smooth muscle cells, fibroblasts and BMSCs were used as seed cells.
27425182	4	69	used	used	656:659	arg2	cells					669:673	seed cells	664:673	seed cells	664:673	METHODS Collagen-chitosan composite material was used as the cell matrix, and smooth muscle cells, fibroblasts and BMSCs were used as seed cells.
27425182	4	70	theme	composite	556:564	arg1	material					566:573	METHODS Collagen-chitosan composite material	530:573	METHODS Collagen-chitosan composite material	530:573	METHODS Collagen-chitosan composite material was used as the cell matrix, and smooth muscle cells, fibroblasts and BMSCs were used as seed cells.
27425182	4	70	theme	composite	556:564	arg1	matrix					596:601	the cell matrix	587:601	the cell matrix	587:601	METHODS Collagen-chitosan composite material was used as the cell matrix, and smooth muscle cells, fibroblasts and BMSCs were used as seed cells.
27425182	6	71	from	proliferation	1006:1018	arg1	material					1055:1062	the collagen-chitosan composite material	1023:1062	the collagen-chitosan composite material	1023:1062	After four weeks in culture, the cellular density and activity were assessed on segments of the tissue engineered heart valve scaffolds to determine the cell viability and proliferation in the collagen-chitosan composite material.
27425182	5	72	theme	muscle	725:730	arg1	cells					732:736	the smooth muscle cells	714:736	the smooth muscle cells	714:736	After four weeks of in vitro culture, the smooth muscle cells, fibroblasts, and BMSCs were sequentially seeded into the collagen-chitosan composite material.
27425182	9	73	theme	similar	1533:1539	arg1	shapes					1526:1531	shapes	1526:1531	shapes similar to the morphology of smooth muscle cells, fibroblasts, and endothelial cells	1526:1616	Light and electron microscopy showed that the seeded cells had shapes similar to the morphology of smooth muscle cells, fibroblasts, and endothelial cells.
27425182	6	74	theme	cell	987:990	arg1	viability					992:1000	cell viability	987:1000	cell viability	987:1000	After four weeks in culture, the cellular density and activity were assessed on segments of the tissue engineered heart valve scaffolds to determine the cell viability and proliferation in the collagen-chitosan composite material.
27425182	3	75	theme	biological	464:473	arg1	activities					475:484	biological activities	464:484	biological activities	464:484	The adhesion and biological activities of the seeded cells were also investigated.
27425182	0	76	theme	Chitosan-Collagen	11:27	arg1	Composites					29:38	Degradable Chitosan-Collagen Composites	0:38	Degradable Chitosan-Collagen Composites	0:38	Degradable Chitosan-Collagen Composites Seeded with Cells as Tissue Engineered Heart Valves.
27425182	1	77	theme	BACKGROUND	93:102	arg1	materials					143:151	BACKGROUND Degradable collagen-chitosan composite materials	93:151	BACKGROUND Degradable collagen-chitosan composite materials	93:151	BACKGROUND Degradable collagen-chitosan composite materials have been used to fabricate tissue engineered heart valves.
27425182	4	78	theme	cell	591:594	arg1	material					566:573	METHODS Collagen-chitosan composite material	530:573	METHODS Collagen-chitosan composite material	530:573	METHODS Collagen-chitosan composite material was used as the cell matrix, and smooth muscle cells, fibroblasts and BMSCs were used as seed cells.
27425182	4	78	theme	cell	591:594	arg1	matrix					596:601	the cell matrix	587:601	the cell matrix	587:601	METHODS Collagen-chitosan composite material was used as the cell matrix, and smooth muscle cells, fibroblasts and BMSCs were used as seed cells.
27425182	6	79	from	viability	992:1000	arg1	material					1055:1062	the collagen-chitosan composite material	1023:1062	the collagen-chitosan composite material	1023:1062	After four weeks in culture, the cellular density and activity were assessed on segments of the tissue engineered heart valve scaffolds to determine the cell viability and proliferation in the collagen-chitosan composite material.
27425182	1	80	dep	engineered	188:197	arg1	tissue					181:186	tissue	181:186	tissue	181:186	BACKGROUND Degradable collagen-chitosan composite materials have been used to fabricate tissue engineered heart valves.
27425182	10	81	theme	Endothelial	1631:1641	arg1	cells					1643:1647	Endothelial cells	1631:1647	Endothelial cells	1631:1647	CONCLUSIONS Endothelial cells can be differentiated from BMSCs when seeded onto the collagen-chitosan composite scaffolds.
27425182	9	82	theme	muscle	1569:1574	arg1	cells					1576:1580	smooth muscle cells	1562:1580	smooth muscle cells	1562:1580	Light and electron microscopy showed that the seeded cells had shapes similar to the morphology of smooth muscle cells, fibroblasts, and endothelial cells.
27425182	8	83	theme	culture	1392:1398	arg1	fluid					1400:1404	the collagen-chitosan cell culture fluid	1365:1404	the collagen-chitosan cell culture fluid	1365:1404	The 6-ketone prostaglandin content, as measured by radioimmunoassay, of the collagen-chitosan cell culture fluid was higher than that of the serum-free medium (P <0.01).
27425182	3	84	theme	seeded	493:498	arg1	cells					500:504	the seeded cells	489:504	the seeded cells	489:504	The adhesion and biological activities of the seeded cells were also investigated.
27425182	7	85	theme	cell	1172:1175	arg1	VIII					1184:1187	endothelial cell factor VIII	1160:1187	endothelial cell factor VIII	1160:1187	RESULTS The tissue engineered heart valves stained positively for both smooth muscle actin and endothelial cell factor VIII, suggesting that the seeded cells were in fact smooth muscle cells, fibroblasts, and endothelial cells.
27425182	7	86	theme	tissue	1077:1082	arg1	valves					1101:1106	The tissue engineered heart valves	1073:1106	RESULTS The tissue engineered heart valves	1065:1106	RESULTS The tissue engineered heart valves stained positively for both smooth muscle actin and endothelial cell factor VIII, suggesting that the seeded cells were in fact smooth muscle cells, fibroblasts, and endothelial cells.
27425182	9	87	contain	had	1522:1524	arg2	shapes					1526:1531	shapes	1526:1531	shapes similar to the morphology of smooth muscle cells, fibroblasts, and endothelial cells	1526:1616	Light and electron microscopy showed that the seeded cells had shapes similar to the morphology of smooth muscle cells, fibroblasts, and endothelial cells.
27425182	9	87	contain	had	1522:1524	arg1	cells					1516:1520	the seeded cells	1505:1520	the seeded cells	1505:1520	Light and electron microscopy showed that the seeded cells had shapes similar to the morphology of smooth muscle cells, fibroblasts, and endothelial cells.
27425182	2	88	theme	study	230:234	arg1	aims					217:220	The aims	213:220	The aims of this study	213:234	The aims of this study were to demonstrate that the collagen-chitosan composite scaffolds are cytocompatible, and endothelial cells can be differentiated from bone marrow mesenchymal stem cells (BMSCs) when seeded onto the scaffolds.
27425182	2	89	theme	bone	372:375	arg1	marrow					377:382	bone marrow mesenchymal stem cells (BMSCs)	372:413	bone marrow mesenchymal stem cells (BMSCs)	372:413	The aims of this study were to demonstrate that the collagen-chitosan composite scaffolds are cytocompatible, and endothelial cells can be differentiated from bone marrow mesenchymal stem cells (BMSCs) when seeded onto the scaffolds.
27425182	9	90	theme	endothelial	1600:1610	arg1	cells					1612:1616	endothelial cells	1600:1616	endothelial cells	1600:1616	Light and electron microscopy showed that the seeded cells had shapes similar to the morphology of smooth muscle cells, fibroblasts, and endothelial cells.
27425182	8	91	theme	serum-free	1434:1443	arg1	<0.01					1455:1459	P <0.01	1453:1459	P <0.01	1453:1459	The 6-ketone prostaglandin content, as measured by radioimmunoassay, of the collagen-chitosan cell culture fluid was higher than that of the serum-free medium (P <0.01).
27425182	8	91	theme	serum-free	1434:1443	arg1	medium					1445:1450	the serum-free medium	1430:1450	the serum-free medium (P <0.01)	1430:1460	The 6-ketone prostaglandin content, as measured by radioimmunoassay, of the collagen-chitosan cell culture fluid was higher than that of the serum-free medium (P <0.01).
27425182	5	92	theme	collagen-chitosan	796:812	arg1	material					824:831	the collagen-chitosan composite material	792:831	the collagen-chitosan composite material	792:831	After four weeks of in vitro culture, the smooth muscle cells, fibroblasts, and BMSCs were sequentially seeded into the collagen-chitosan composite material.
24415556	7	0	with	complex	1303:1309	arg1	isoforms					1321:1328	both isoforms	1316:1328	both isoforms of UGGT	1316:1336	Finally, Sep15 was found to form the heterodimeric complex with both isoforms of UGGT and markedly enhanced its glucosyltransferase activity.
24415556	4	1	theme	other	657:661	arg1	hand					663:666	the other hand	653:666	the other hand	653:666	On the other hand, direct evidence to support the glucosyltransferase activity of UGGT2 has been lacking, leaving it unclear as to whether it has any function in the glycoprotein folding process.
24415556	4	2	theme	glycoprotein	816:827	arg1	process					837:843	the glycoprotein folding process	812:843	the glycoprotein folding process	812:843	On the other hand, direct evidence to support the glucosyltransferase activity of UGGT2 has been lacking, leaving it unclear as to whether it has any function in the glycoprotein folding process.
24415556	1	3	theme	cycle	167:171	arg1	constituent					127:137	an important constituent	114:137	an important constituent of the glycoprotein folding cycle	114:171	Being recognized as an important constituent of the glycoprotein folding cycle, uridine diphosphate-glucose:glycoprotein glucosyltransferase (UGGT) has been a subject of intense study.
24415556	1	3	theme	cycle	167:171	arg1	diphosphate-glucose					182:200	uridine diphosphate-glucose	174:200	uridine diphosphate-glucose:glycoprotein glucosyltransferase (UGGT) has been a subject of intense study	174:276	Being recognized as an important constituent of the glycoprotein folding cycle, uridine diphosphate-glucose:glycoprotein glucosyltransferase (UGGT) has been a subject of intense study.
24415556	6	4	theme	non-native	1226:1235	arg1	conformations					1237:1249	non-native conformations	1226:1249	non-native conformations	1226:1249	The analysis, for the first time, revealed the glucosyltransferase activity of UGGT2, whose specificity was shown to be quite similar to UGGT1, in terms of both glycan specificity and preferential recognition of proteins having non-native conformations.
24415556	2	5	dep	now	285:287	arg1	Up					279:280	Up	279:280	Up	279:280	Up to now, it is two isoforms, UGGT1 and 2 have been identified, which share ∼ 50% amino acid identity.
24415556	3	6	gly	glycoproteins	600:612	arg1	glycoproteins					600:612	immature glycoproteins	591:612	immature glycoproteins exhibiting non-native conformation	591:647	UGGT1 is a well-documented enzyme which functions as a folding sensor in the endoplasmic reticulum, by the virtue of its ability to transfer a glucose residue to non-glucosylated high-mannose-type glycans of immature glycoproteins exhibiting non-native conformation.
24415556	1	7	gly	glycoprotein	202:213	arg1	glycoprotein					202:213	glycoprotein glucosyltransferase	202:233	glycoprotein glucosyltransferase (UGGT)	202:240	Being recognized as an important constituent of the glycoprotein folding cycle, uridine diphosphate-glucose:glycoprotein glucosyltransferase (UGGT) has been a subject of intense study.
24415556	3	8	theme	endoplasmic	460:470	arg1	reticulum					472:480	the endoplasmic reticulum	456:480	the endoplasmic reticulum	456:480	UGGT1 is a well-documented enzyme which functions as a folding sensor in the endoplasmic reticulum, by the virtue of its ability to transfer a glucose residue to non-glucosylated high-mannose-type glycans of immature glycoproteins exhibiting non-native conformation.
24415556	1	9	theme	study	272:276	arg1	subject					253:259	a subject	251:259	a subject of intense study	251:276	Being recognized as an important constituent of the glycoprotein folding cycle, uridine diphosphate-glucose:glycoprotein glucosyltransferase (UGGT) has been a subject of intense study.
24415556	1	9	theme	study	272:276	arg1	glucosyltransferase					215:233	glycoprotein glucosyltransferase	202:233	glycoprotein glucosyltransferase (UGGT)	202:240	Being recognized as an important constituent of the glycoprotein folding cycle, uridine diphosphate-glucose:glycoprotein glucosyltransferase (UGGT) has been a subject of intense study.
24415556	4	10	theme	glucosyltransferase	700:718	arg1	activity					720:727	the glucosyltransferase activity	696:727	the glucosyltransferase activity of UGGT2	696:736	On the other hand, direct evidence to support the glucosyltransferase activity of UGGT2 has been lacking, leaving it unclear as to whether it has any function in the glycoprotein folding process.
24415556	4	11	theme	folding	829:835	arg1	process					837:843	the glycoprotein folding process	812:843	the glycoprotein folding process	812:843	On the other hand, direct evidence to support the glucosyltransferase activity of UGGT2 has been lacking, leaving it unclear as to whether it has any function in the glycoprotein folding process.
24415556	6	12	theme	proteins	1210:1217	arg1	recognition					1195:1205	preferential recognition	1182:1205	preferential recognition	1182:1205	The analysis, for the first time, revealed the glucosyltransferase activity of UGGT2, whose specificity was shown to be quite similar to UGGT1, in terms of both glycan specificity and preferential recognition of proteins having non-native conformations.
24415556	6	12	theme	proteins	1210:1217	arg1	specificity					1166:1176	glycan specificity	1159:1176	glycan specificity	1159:1176	The analysis, for the first time, revealed the glucosyltransferase activity of UGGT2, whose specificity was shown to be quite similar to UGGT1, in terms of both glycan specificity and preferential recognition of proteins having non-native conformations.
24415556	4	13	theme	UGGT2	732:736	arg1	activity					720:727	the glucosyltransferase activity	696:727	the glucosyltransferase activity of UGGT2	696:736	On the other hand, direct evidence to support the glucosyltransferase activity of UGGT2 has been lacking, leaving it unclear as to whether it has any function in the glycoprotein folding process.
24415556	3	14	theme	folding	438:444	arg1	enzyme					410:415	a well-documented enzyme	392:415	a well-documented enzyme which functions as a folding sensor in the endoplasmic reticulum, by the virtue of its ability to transfer a glucose residue to non-glucosylated high-mannose-type glycans of immature glycoproteins exhibiting non-native conformation	392:647	UGGT1 is a well-documented enzyme which functions as a folding sensor in the endoplasmic reticulum, by the virtue of its ability to transfer a glucose residue to non-glucosylated high-mannose-type glycans of immature glycoproteins exhibiting non-native conformation.
24415556	3	14	theme	folding	438:444	arg1	sensor					446:451	a folding sensor	436:451	a folding sensor in the endoplasmic reticulum	436:480	UGGT1 is a well-documented enzyme which functions as a folding sensor in the endoplasmic reticulum, by the virtue of its ability to transfer a glucose residue to non-glucosylated high-mannose-type glycans of immature glycoproteins exhibiting non-native conformation.
24415556	7	15	theme	UGGT	1333:1336	arg1	isoforms					1321:1328	both isoforms	1316:1328	both isoforms of UGGT	1316:1336	Finally, Sep15 was found to form the heterodimeric complex with both isoforms of UGGT and markedly enhanced its glucosyltransferase activity.
24415556	7	16	theme	glucosyltransferase	1364:1382	arg1	activity					1384:1391	its glucosyltransferase activity	1360:1391	its glucosyltransferase activity	1360:1391	Finally, Sep15 was found to form the heterodimeric complex with both isoforms of UGGT and markedly enhanced its glucosyltransferase activity.
24415556	6	17	contain	having	1219:1224	arg2	conformations					1237:1249	non-native conformations	1226:1249	non-native conformations	1226:1249	The analysis, for the first time, revealed the glucosyltransferase activity of UGGT2, whose specificity was shown to be quite similar to UGGT1, in terms of both glycan specificity and preferential recognition of proteins having non-native conformations.
24415556	6	17	contain	having	1219:1224	arg1	proteins					1210:1217	proteins	1210:1217	proteins having non-native conformations	1210:1249	The analysis, for the first time, revealed the glucosyltransferase activity of UGGT2, whose specificity was shown to be quite similar to UGGT1, in terms of both glycan specificity and preferential recognition of proteins having non-native conformations.
24415556	1	18	theme	uridine	174:180	arg1	diphosphate-glucose					182:200	uridine diphosphate-glucose	174:200	uridine diphosphate-glucose:glycoprotein glucosyltransferase (UGGT) has been a subject of intense study	174:276	Being recognized as an important constituent of the glycoprotein folding cycle, uridine diphosphate-glucose:glycoprotein glucosyltransferase (UGGT) has been a subject of intense study.
24415556	1	18	theme	uridine	174:180	arg1	constituent					127:137	an important constituent	114:137	an important constituent of the glycoprotein folding cycle	114:171	Being recognized as an important constituent of the glycoprotein folding cycle, uridine diphosphate-glucose:glycoprotein glucosyltransferase (UGGT) has been a subject of intense study.
24415556	6	19	theme	recognition	1195:1205	arg1	terms					1145:1149	terms	1145:1149	terms of both glycan specificity and preferential recognition of proteins having non-native conformations	1145:1249	The analysis, for the first time, revealed the glucosyltransferase activity of UGGT2, whose specificity was shown to be quite similar to UGGT1, in terms of both glycan specificity and preferential recognition of proteins having non-native conformations.
24415556	4	20	gly	glycoprotein	816:827	arg1	glycoprotein					816:827	the glycoprotein folding process	812:843	the glycoprotein folding process	812:843	On the other hand, direct evidence to support the glucosyltransferase activity of UGGT2 has been lacking, leaving it unclear as to whether it has any function in the glycoprotein folding process.
24415556	5	21	gly	N-glycosylated	973:986	arg1	proteins					988:995	N-glycosylated proteins	973:995	N-glycosylated proteins	973:995	This study aimed to reveal the property of human UGGT2 by using synthetic substrates such as fluorescently labeled glycans and N-glycosylated proteins.
24415556	6	22	theme	preferential	1182:1193	arg1	recognition					1195:1205	preferential recognition	1182:1205	preferential recognition	1182:1205	The analysis, for the first time, revealed the glucosyltransferase activity of UGGT2, whose specificity was shown to be quite similar to UGGT1, in terms of both glycan specificity and preferential recognition of proteins having non-native conformations.
24415556	3	23	theme	ability	504:510	arg1	virtue					490:495	the virtue	486:495	the virtue of its ability to transfer a glucose residue to non-glucosylated high-mannose-type glycans of immature glycoproteins exhibiting non-native conformation	486:647	UGGT1 is a well-documented enzyme which functions as a folding sensor in the endoplasmic reticulum, by the virtue of its ability to transfer a glucose residue to non-glucosylated high-mannose-type glycans of immature glycoproteins exhibiting non-native conformation.
24415556	4	24	from	function	800:807	arg1	process					837:843	the glycoprotein folding process	812:843	the glycoprotein folding process	812:843	On the other hand, direct evidence to support the glucosyltransferase activity of UGGT2 has been lacking, leaving it unclear as to whether it has any function in the glycoprotein folding process.
24415556	6	25	theme	first	1020:1024	arg1	time					1026:1029	the first time	1016:1029	the first time	1016:1029	The analysis, for the first time, revealed the glucosyltransferase activity of UGGT2, whose specificity was shown to be quite similar to UGGT1, in terms of both glycan specificity and preferential recognition of proteins having non-native conformations.
24415556	5	26	theme	human	889:893	arg1	UGGT2					895:899	human UGGT2	889:899	human UGGT2	889:899	This study aimed to reveal the property of human UGGT2 by using synthetic substrates such as fluorescently labeled glycans and N-glycosylated proteins.
24415556	1	27	theme	glycoprotein	202:213	arg1	subject					253:259	a subject	251:259	a subject of intense study	251:276	Being recognized as an important constituent of the glycoprotein folding cycle, uridine diphosphate-glucose:glycoprotein glucosyltransferase (UGGT) has been a subject of intense study.
24415556	1	27	theme	glycoprotein	202:213	arg1	UGGT					236:239	UGGT	236:239	UGGT	236:239	Being recognized as an important constituent of the glycoprotein folding cycle, uridine diphosphate-glucose:glycoprotein glucosyltransferase (UGGT) has been a subject of intense study.
24415556	1	27	theme	glycoprotein	202:213	arg1	glucosyltransferase					215:233	glycoprotein glucosyltransferase	202:233	glycoprotein glucosyltransferase (UGGT)	202:240	Being recognized as an important constituent of the glycoprotein folding cycle, uridine diphosphate-glucose:glycoprotein glucosyltransferase (UGGT) has been a subject of intense study.
24415556	0	28	theme	human	17:21	arg1	glucosyltransferase					48:66	human UDP-glucose:glycoprotein glucosyltransferase	17:66	human UDP-glucose:glycoprotein glucosyltransferase	17:66	Both isoforms of human UDP-glucose:glycoprotein glucosyltransferase are enzymatically active.
24415556	5	29	theme	labeled	953:959	arg1	glycans					961:967	fluorescently labeled glycans	939:967	fluorescently labeled glycans	939:967	This study aimed to reveal the property of human UGGT2 by using synthetic substrates such as fluorescently labeled glycans and N-glycosylated proteins.
24415556	3	30	from	sensor	446:451	arg1	reticulum					472:480	the endoplasmic reticulum	456:480	the endoplasmic reticulum	456:480	UGGT1 is a well-documented enzyme which functions as a folding sensor in the endoplasmic reticulum, by the virtue of its ability to transfer a glucose residue to non-glucosylated high-mannose-type glycans of immature glycoproteins exhibiting non-native conformation.
24415556	4	31	dep	evidence	676:683	arg1	support					688:694	support	688:694	to support the glucosyltransferase activity of UGGT2	685:736	On the other hand, direct evidence to support the glucosyltransferase activity of UGGT2 has been lacking, leaving it unclear as to whether it has any function in the glycoprotein folding process.
24415556	6	32	theme	specificity	1166:1176	arg1	terms					1145:1149	terms	1145:1149	terms of both glycan specificity and preferential recognition of proteins having non-native conformations	1145:1249	The analysis, for the first time, revealed the glucosyltransferase activity of UGGT2, whose specificity was shown to be quite similar to UGGT1, in terms of both glycan specificity and preferential recognition of proteins having non-native conformations.
24415556	3	33	theme	glycoproteins	600:612	arg1	glycans					580:586	non-glucosylated high-mannose-type glycans	545:586	non-glucosylated high-mannose-type glycans of immature glycoproteins exhibiting non-native conformation	545:647	UGGT1 is a well-documented enzyme which functions as a folding sensor in the endoplasmic reticulum, by the virtue of its ability to transfer a glucose residue to non-glucosylated high-mannose-type glycans of immature glycoproteins exhibiting non-native conformation.
24415556	2	34	theme	acid	368:371	arg1	identity					373:380	∼ 50% amino acid identity	356:380	∼ 50% amino acid identity	356:380	Up to now, it is two isoforms, UGGT1 and 2 have been identified, which share ∼ 50% amino acid identity.
24415556	3	35	theme	well-documented	394:408	arg1	enzyme					410:415	a well-documented enzyme	392:415	a well-documented enzyme which functions as a folding sensor in the endoplasmic reticulum, by the virtue of its ability to transfer a glucose residue to non-glucosylated high-mannose-type glycans of immature glycoproteins exhibiting non-native conformation	392:647	UGGT1 is a well-documented enzyme which functions as a folding sensor in the endoplasmic reticulum, by the virtue of its ability to transfer a glucose residue to non-glucosylated high-mannose-type glycans of immature glycoproteins exhibiting non-native conformation.
24415556	3	35	theme	well-documented	394:408	arg1	sensor					446:451	a folding sensor	436:451	a folding sensor in the endoplasmic reticulum	436:480	UGGT1 is a well-documented enzyme which functions as a folding sensor in the endoplasmic reticulum, by the virtue of its ability to transfer a glucose residue to non-glucosylated high-mannose-type glycans of immature glycoproteins exhibiting non-native conformation.
24415556	3	35	theme	well-documented	394:408	arg1	UGGT1					383:387	UGGT1	383:387	UGGT1	383:387	UGGT1 is a well-documented enzyme which functions as a folding sensor in the endoplasmic reticulum, by the virtue of its ability to transfer a glucose residue to non-glucosylated high-mannose-type glycans of immature glycoproteins exhibiting non-native conformation.
24415556	3	36	theme	glucose	526:532	arg1	residue					534:540	a glucose residue	524:540	a glucose residue	524:540	UGGT1 is a well-documented enzyme which functions as a folding sensor in the endoplasmic reticulum, by the virtue of its ability to transfer a glucose residue to non-glucosylated high-mannose-type glycans of immature glycoproteins exhibiting non-native conformation.
24415556	2	37	theme	amino	362:366	arg1	identity					373:380	∼ 50% amino acid identity	356:380	∼ 50% amino acid identity	356:380	Up to now, it is two isoforms, UGGT1 and 2 have been identified, which share ∼ 50% amino acid identity.
24415556	0	38	theme	UDP-glucose	23:33	arg1	glucosyltransferase					48:66	human UDP-glucose:glycoprotein glucosyltransferase	17:66	human UDP-glucose:glycoprotein glucosyltransferase	17:66	Both isoforms of human UDP-glucose:glycoprotein glucosyltransferase are enzymatically active.
24415556	1	39	theme	intense	264:270	arg1	study					272:276	intense study	264:276	intense study	264:276	Being recognized as an important constituent of the glycoprotein folding cycle, uridine diphosphate-glucose:glycoprotein glucosyltransferase (UGGT) has been a subject of intense study.
24415556	2	40	dep	%	360:360	arg1	50					358:359	50	358:359	50	358:359	Up to now, it is two isoforms, UGGT1 and 2 have been identified, which share ∼ 50% amino acid identity.
24415556	0	41	theme	glucosyltransferase	48:66	arg1	isoforms					5:12	Both isoforms	0:12	Both isoforms of human UDP-glucose:glycoprotein glucosyltransferase	0:66	Both isoforms of human UDP-glucose:glycoprotein glucosyltransferase are enzymatically active.
24415556	3	42	theme	immature	591:598	arg1	glycoproteins					600:612	immature glycoproteins	591:612	immature glycoproteins exhibiting non-native conformation	591:647	UGGT1 is a well-documented enzyme which functions as a folding sensor in the endoplasmic reticulum, by the virtue of its ability to transfer a glucose residue to non-glucosylated high-mannose-type glycans of immature glycoproteins exhibiting non-native conformation.
24415556	2	43	dep	identity	373:380	arg1	%					360:360	%	360:360	%	360:360	Up to now, it is two isoforms, UGGT1 and 2 have been identified, which share ∼ 50% amino acid identity.
24415556	1	44	theme	important	117:125	arg1	constituent					127:137	an important constituent	114:137	an important constituent of the glycoprotein folding cycle	114:171	Being recognized as an important constituent of the glycoprotein folding cycle, uridine diphosphate-glucose:glycoprotein glucosyltransferase (UGGT) has been a subject of intense study.
24415556	1	44	theme	important	117:125	arg1	diphosphate-glucose					182:200	uridine diphosphate-glucose	174:200	uridine diphosphate-glucose:glycoprotein glucosyltransferase (UGGT) has been a subject of intense study	174:276	Being recognized as an important constituent of the glycoprotein folding cycle, uridine diphosphate-glucose:glycoprotein glucosyltransferase (UGGT) has been a subject of intense study.
24415556	0	45	theme	glycoprotein	35:46	arg1	glucosyltransferase					48:66	human UDP-glucose:glycoprotein glucosyltransferase	17:66	human UDP-glucose:glycoprotein glucosyltransferase	17:66	Both isoforms of human UDP-glucose:glycoprotein glucosyltransferase are enzymatically active.
24415556	6	46	theme	glycan	1159:1164	arg1	specificity					1166:1176	glycan specificity	1159:1176	glycan specificity	1159:1176	The analysis, for the first time, revealed the glucosyltransferase activity of UGGT2, whose specificity was shown to be quite similar to UGGT1, in terms of both glycan specificity and preferential recognition of proteins having non-native conformations.
24415556	1	47	gly	glycoprotein	146:157	arg1	glycoprotein					146:157	the glycoprotein folding cycle	142:171	the glycoprotein folding cycle	142:171	Being recognized as an important constituent of the glycoprotein folding cycle, uridine diphosphate-glucose:glycoprotein glucosyltransferase (UGGT) has been a subject of intense study.
24415556	1	48	dep	diphosphate-glucose	182:200	arg1	subject					253:259	a subject	251:259	a subject of intense study	251:276	Being recognized as an important constituent of the glycoprotein folding cycle, uridine diphosphate-glucose:glycoprotein glucosyltransferase (UGGT) has been a subject of intense study.
24415556	1	48	dep	diphosphate-glucose	182:200	arg1	glucosyltransferase					215:233	glycoprotein glucosyltransferase	202:233	glycoprotein glucosyltransferase (UGGT)	202:240	Being recognized as an important constituent of the glycoprotein folding cycle, uridine diphosphate-glucose:glycoprotein glucosyltransferase (UGGT) has been a subject of intense study.
24415556	4	49	theme	direct	669:674	arg1	evidence					676:683	direct evidence	669:683	direct evidence to support the glucosyltransferase activity of UGGT2	669:736	On the other hand, direct evidence to support the glucosyltransferase activity of UGGT2 has been lacking, leaving it unclear as to whether it has any function in the glycoprotein folding process.
24415556	7	50	theme	heterodimeric	1289:1301	arg1	complex					1303:1309	the heterodimeric complex	1285:1309	the heterodimeric complex with both isoforms of UGGT	1285:1336	Finally, Sep15 was found to form the heterodimeric complex with both isoforms of UGGT and markedly enhanced its glucosyltransferase activity.
24415556	3	51	theme	high-mannose-type	562:578	arg1	glycans					580:586	non-glucosylated high-mannose-type glycans	545:586	non-glucosylated high-mannose-type glycans of immature glycoproteins exhibiting non-native conformation	545:647	UGGT1 is a well-documented enzyme which functions as a folding sensor in the endoplasmic reticulum, by the virtue of its ability to transfer a glucose residue to non-glucosylated high-mannose-type glycans of immature glycoproteins exhibiting non-native conformation.
24415556	5	52	theme	N-glycosylated	973:986	arg1	proteins					988:995	N-glycosylated proteins	973:995	N-glycosylated proteins	973:995	This study aimed to reveal the property of human UGGT2 by using synthetic substrates such as fluorescently labeled glycans and N-glycosylated proteins.
24415556	6	53	theme	UGGT2	1077:1081	arg1	activity					1065:1072	the glucosyltransferase activity	1041:1072	the glucosyltransferase activity	1041:1072	The analysis, for the first time, revealed the glucosyltransferase activity of UGGT2, whose specificity was shown to be quite similar to UGGT1, in terms of both glycan specificity and preferential recognition of proteins having non-native conformations.
24415556	5	54	theme	UGGT2	895:899	arg1	property					877:884	the property	873:884	the property of human UGGT2	873:899	This study aimed to reveal the property of human UGGT2 by using synthetic substrates such as fluorescently labeled glycans and N-glycosylated proteins.
24415556	3	55	theme	non-native	625:634	arg1	conformation					636:647	non-native conformation	625:647	non-native conformation	625:647	UGGT1 is a well-documented enzyme which functions as a folding sensor in the endoplasmic reticulum, by the virtue of its ability to transfer a glucose residue to non-glucosylated high-mannose-type glycans of immature glycoproteins exhibiting non-native conformation.
24415556	6	56	theme	glucosyltransferase	1045:1063	arg1	activity					1065:1072	the glucosyltransferase activity	1041:1072	the glucosyltransferase activity	1041:1072	The analysis, for the first time, revealed the glucosyltransferase activity of UGGT2, whose specificity was shown to be quite similar to UGGT1, in terms of both glycan specificity and preferential recognition of proteins having non-native conformations.
24415556	4	57	contain	has	792:794	arg2	function					800:807	any function	796:807	any function in the glycoprotein folding process	796:843	On the other hand, direct evidence to support the glucosyltransferase activity of UGGT2 has been lacking, leaving it unclear as to whether it has any function in the glycoprotein folding process.
24415556	4	57	contain	has	792:794	arg1	it					789:790	it	789:790	it	789:790	On the other hand, direct evidence to support the glucosyltransferase activity of UGGT2 has been lacking, leaving it unclear as to whether it has any function in the glycoprotein folding process.
24415556	0	58	gly	glycoprotein	35:46	arg1	glycoprotein					35:46	human UDP-glucose:glycoprotein glucosyltransferase	17:66	human UDP-glucose:glycoprotein glucosyltransferase	17:66	Both isoforms of human UDP-glucose:glycoprotein glucosyltransferase are enzymatically active.
24415556	1	59	theme	glycoprotein	146:157	arg1	cycle					167:171	the glycoprotein folding cycle	142:171	the glycoprotein folding cycle	142:171	Being recognized as an important constituent of the glycoprotein folding cycle, uridine diphosphate-glucose:glycoprotein glucosyltransferase (UGGT) has been a subject of intense study.
24415556	5	60	theme	synthetic	910:918	arg1	proteins					988:995	N-glycosylated proteins	973:995	N-glycosylated proteins	973:995	This study aimed to reveal the property of human UGGT2 by using synthetic substrates such as fluorescently labeled glycans and N-glycosylated proteins.
24415556	5	60	theme	synthetic	910:918	arg1	glycans					961:967	fluorescently labeled glycans	939:967	fluorescently labeled glycans	939:967	This study aimed to reveal the property of human UGGT2 by using synthetic substrates such as fluorescently labeled glycans and N-glycosylated proteins.
24415556	5	60	theme	synthetic	910:918	arg1	substrates					920:929	synthetic substrates	910:929	synthetic substrates such as fluorescently labeled glycans and N-glycosylated proteins	910:995	This study aimed to reveal the property of human UGGT2 by using synthetic substrates such as fluorescently labeled glycans and N-glycosylated proteins.
24415556	3	61	theme	non-glucosylated	545:560	arg1	glycans					580:586	non-glucosylated high-mannose-type glycans	545:586	non-glucosylated high-mannose-type glycans of immature glycoproteins exhibiting non-native conformation	545:647	UGGT1 is a well-documented enzyme which functions as a folding sensor in the endoplasmic reticulum, by the virtue of its ability to transfer a glucose residue to non-glucosylated high-mannose-type glycans of immature glycoproteins exhibiting non-native conformation.
24415556	1	62	theme	folding	159:165	arg1	cycle					167:171	the glycoprotein folding cycle	142:171	the glycoprotein folding cycle	142:171	Being recognized as an important constituent of the glycoprotein folding cycle, uridine diphosphate-glucose:glycoprotein glucosyltransferase (UGGT) has been a subject of intense study.
26404269	7	0	theme	current	1179:1185	arg1	response					1187:1194	The current response	1175:1194	The current response	1175:1194	The current response was maintained over 95% of the initial value after 17 days, and the current difference measurement obtained using different electrodes provided a relative standard deviation (RSD) of 4.47%.
26404269	5	1	theme	real	1085:1088	arg1	samples					1090:1096	real samples	1085:1096	real samples	1085:1096	The modified glucose biosensor exhibits a limit of detection (LOD) of (50 ± 10) µM and also excludes interfering species, such as ascorbic acid, uric acid, and cholesterol, which makes this sensor suitable for glucose determination in real samples.
26404269	7	2	theme	relative	1342:1349	arg1	RSD					1371:1373	RSD	1371:1373	RSD	1371:1373	The current response was maintained over 95% of the initial value after 17 days, and the current difference measurement obtained using different electrodes provided a relative standard deviation (RSD) of 4.47%.
26404269	7	2	theme	relative	1342:1349	arg1	deviation					1360:1368	a relative standard deviation	1340:1368	a relative standard deviation (RSD) of 4.47%	1340:1383	The current response was maintained over 95% of the initial value after 17 days, and the current difference measurement obtained using different electrodes provided a relative standard deviation (RSD) of 4.47%.
26404269	0	3	theme	Glucose	76:82	arg1	Detection					63:71	the Biosensing Detection	48:71	the Biosensing Detection of Glucose	48:82	Synergy Effect of Nanocrystalline Cellulose for the Biosensing Detection of Glucose.
26404269	4	4	theme	native	631:636	arg1	structure					638:646	the native structure	627:646	the native structure	627:646	The voltammetric results showed that the native structure and biocatalytic activity of GOx immobilized on the PPy-CNC nanocomposite remained and exhibited a high sensitivity (ca. 0.73 μA·mM(-1)), with a high dynamic response ranging from 1.0 to 20 mM glucose.
26404269	3	5	theme	emission	401:408	arg1	FESEM					440:444	FESEM	440:444	FESEM	440:444	Field emission scanning electron microscopy (FESEM) images showed that fibers were nanosized and porous, which is appropriate for accommodating enzymes and increasing electron transfer kinetics.
26404269	3	5	theme	emission	401:408	arg1	microscopy					428:437	Field emission scanning electron microscopy	395:437	Field emission scanning electron microscopy (FESEM) images	395:452	Field emission scanning electron microscopy (FESEM) images showed that fibers were nanosized and porous, which is appropriate for accommodating enzymes and increasing electron transfer kinetics.
26404269	7	6	theme	difference	1272:1281	arg1	measurement					1283:1293	the current difference measurement	1260:1293	the current difference measurement obtained using different electrodes	1260:1329	The current response was maintained over 95% of the initial value after 17 days, and the current difference measurement obtained using different electrodes provided a relative standard deviation (RSD) of 4.47%.
26404269	6	7	theme	acceptable	1123:1132	arg1	reproducibility					1134:1148	reproducibility	1134:1148	reproducibility	1134:1148	This sensor displays an acceptable reproducibility and stability over time.
26404269	2	8	theme	unique	317:322	arg1	properties					340:349	unique physicochemical properties	317:349	unique physicochemical properties	317:349	Polypyrrole-cellulose nanocrystal (PPy-CNC)-based composite as a novel immobilization membrane with unique physicochemical properties was found to enhance biosensor performance.
26404269	4	9	theme	biocatalytic	652:663	arg1	activity					665:672	biocatalytic activity	652:672	biocatalytic activity	652:672	The voltammetric results showed that the native structure and biocatalytic activity of GOx immobilized on the PPy-CNC nanocomposite remained and exhibited a high sensitivity (ca. 0.73 μA·mM(-1)), with a high dynamic response ranging from 1.0 to 20 mM glucose.
26404269	3	10	theme	scanning	410:417	arg1	FESEM					440:444	FESEM	440:444	FESEM	440:444	Field emission scanning electron microscopy (FESEM) images showed that fibers were nanosized and porous, which is appropriate for accommodating enzymes and increasing electron transfer kinetics.
26404269	3	10	theme	scanning	410:417	arg1	microscopy					428:437	Field emission scanning electron microscopy	395:437	Field emission scanning electron microscopy (FESEM) images	395:452	Field emission scanning electron microscopy (FESEM) images showed that fibers were nanosized and porous, which is appropriate for accommodating enzymes and increasing electron transfer kinetics.
26404269	0	11	theme	Synergy	0:6	arg1	Effect					8:13	Synergy Effect	0:13	Synergy Effect of Nanocrystalline Cellulose for the Biosensing Detection of Glucose.	0:83	Synergy Effect of Nanocrystalline Cellulose for the Biosensing Detection of Glucose.
26404269	7	12	theme	initial	1227:1233	arg1	value					1235:1239	the initial value	1223:1239	the initial value after 17 days	1223:1253	The current response was maintained over 95% of the initial value after 17 days, and the current difference measurement obtained using different electrodes provided a relative standard deviation (RSD) of 4.47%.
26404269	7	13	theme	standard	1351:1358	arg1	RSD					1371:1373	RSD	1371:1373	RSD	1371:1373	The current response was maintained over 95% of the initial value after 17 days, and the current difference measurement obtained using different electrodes provided a relative standard deviation (RSD) of 4.47%.
26404269	7	13	theme	standard	1351:1358	arg1	deviation					1360:1368	a relative standard deviation	1340:1368	a relative standard deviation (RSD) of 4.47%	1340:1383	The current response was maintained over 95% of the initial value after 17 days, and the current difference measurement obtained using different electrodes provided a relative standard deviation (RSD) of 4.47%.
26404269	5	14	theme	modified	854:861	arg1	biosensor					871:879	The modified glucose biosensor	850:879	The modified glucose biosensor	850:879	The modified glucose biosensor exhibits a limit of detection (LOD) of (50 ± 10) µM and also excludes interfering species, such as ascorbic acid, uric acid, and cholesterol, which makes this sensor suitable for glucose determination in real samples.
26404269	4	15	dep	sensitivity	752:762	arg1	μA·mM					774:778	0.73 μA·mM	769:778	0.73 μA·mM(-1)	769:782	The voltammetric results showed that the native structure and biocatalytic activity of GOx immobilized on the PPy-CNC nanocomposite remained and exhibited a high sensitivity (ca. 0.73 μA·mM(-1)), with a high dynamic response ranging from 1.0 to 20 mM glucose.
26404269	4	15	dep	sensitivity	752:762	arg1	-1					780:781	-1	780:781	-1	780:781	The voltammetric results showed that the native structure and biocatalytic activity of GOx immobilized on the PPy-CNC nanocomposite remained and exhibited a high sensitivity (ca. 0.73 μA·mM(-1)), with a high dynamic response ranging from 1.0 to 20 mM glucose.
26404269	1	16	theme	new	180:182	arg1	regime					192:197	a new sensing regime	178:197	a new sensing regime	178:197	Integrating polypyrrole-cellulose nanocrystal-based composites with glucose oxidase (GOx) as a new sensing regime was investigated.
26404269	1	16	theme	new	180:182	arg1	composites					137:146	polypyrrole-cellulose nanocrystal-based composites	97:146	polypyrrole-cellulose nanocrystal-based composites with glucose oxidase (GOx)	97:173	Integrating polypyrrole-cellulose nanocrystal-based composites with glucose oxidase (GOx) as a new sensing regime was investigated.
26404269	3	17	theme	Field	395:399	arg1	FESEM					440:444	FESEM	440:444	FESEM	440:444	Field emission scanning electron microscopy (FESEM) images showed that fibers were nanosized and porous, which is appropriate for accommodating enzymes and increasing electron transfer kinetics.
26404269	3	17	theme	Field	395:399	arg1	microscopy					428:437	Field emission scanning electron microscopy	395:437	Field emission scanning electron microscopy (FESEM) images	395:452	Field emission scanning electron microscopy (FESEM) images showed that fibers were nanosized and porous, which is appropriate for accommodating enzymes and increasing electron transfer kinetics.
26404269	5	18	theme	glucose	1060:1066	arg1	determination					1068:1080	glucose determination	1060:1080	glucose determination in real samples	1060:1096	The modified glucose biosensor exhibits a limit of detection (LOD) of (50 ± 10) µM and also excludes interfering species, such as ascorbic acid, uric acid, and cholesterol, which makes this sensor suitable for glucose determination in real samples.
26404269	2	19	theme	immobilization	288:301	arg1	membrane					303:310	a novel immobilization membrane	280:310	a novel immobilization membrane with unique physicochemical properties	280:349	Polypyrrole-cellulose nanocrystal (PPy-CNC)-based composite as a novel immobilization membrane with unique physicochemical properties was found to enhance biosensor performance.
26404269	1	20	theme	sensing	184:190	arg1	regime					192:197	a new sensing regime	178:197	a new sensing regime	178:197	Integrating polypyrrole-cellulose nanocrystal-based composites with glucose oxidase (GOx) as a new sensing regime was investigated.
26404269	1	20	theme	sensing	184:190	arg1	composites					137:146	polypyrrole-cellulose nanocrystal-based composites	97:146	polypyrrole-cellulose nanocrystal-based composites with glucose oxidase (GOx)	97:173	Integrating polypyrrole-cellulose nanocrystal-based composites with glucose oxidase (GOx) as a new sensing regime was investigated.
26404269	1	21	with	composites	137:146	arg1	oxidase					161:167	glucose oxidase	153:167	glucose oxidase (GOx)	153:173	Integrating polypyrrole-cellulose nanocrystal-based composites with glucose oxidase (GOx) as a new sensing regime was investigated.
26404269	1	21	with	composites	137:146	arg1	GOx					170:172	GOx	170:172	GOx	170:172	Integrating polypyrrole-cellulose nanocrystal-based composites with glucose oxidase (GOx) as a new sensing regime was investigated.
26404269	7	22	theme	%	1383:1383	arg1	RSD					1371:1373	RSD	1371:1373	RSD	1371:1373	The current response was maintained over 95% of the initial value after 17 days, and the current difference measurement obtained using different electrodes provided a relative standard deviation (RSD) of 4.47%.
26404269	7	22	theme	%	1383:1383	arg1	deviation					1360:1368	a relative standard deviation	1340:1368	a relative standard deviation (RSD) of 4.47%	1340:1383	The current response was maintained over 95% of the initial value after 17 days, and the current difference measurement obtained using different electrodes provided a relative standard deviation (RSD) of 4.47%.
26404269	1	23	theme	polypyrrole-cellulose	97:117	arg1	regime					192:197	a new sensing regime	178:197	a new sensing regime	178:197	Integrating polypyrrole-cellulose nanocrystal-based composites with glucose oxidase (GOx) as a new sensing regime was investigated.
26404269	1	23	theme	polypyrrole-cellulose	97:117	arg1	composites					137:146	polypyrrole-cellulose nanocrystal-based composites	97:146	polypyrrole-cellulose nanocrystal-based composites with glucose oxidase (GOx)	97:173	Integrating polypyrrole-cellulose nanocrystal-based composites with glucose oxidase (GOx) as a new sensing regime was investigated.
26404269	7	24	theme	current	1264:1270	arg1	measurement					1283:1293	the current difference measurement	1260:1293	the current difference measurement obtained using different electrodes	1260:1329	The current response was maintained over 95% of the initial value after 17 days, and the current difference measurement obtained using different electrodes provided a relative standard deviation (RSD) of 4.47%.
26404269	0	25	theme	Nanocrystalline	18:32	arg1	Cellulose					34:42	Nanocrystalline Cellulose	18:42	Nanocrystalline Cellulose	18:42	Synergy Effect of Nanocrystalline Cellulose for the Biosensing Detection of Glucose.
26404269	1	26	theme	nanocrystal-based	119:135	arg1	regime					192:197	a new sensing regime	178:197	a new sensing regime	178:197	Integrating polypyrrole-cellulose nanocrystal-based composites with glucose oxidase (GOx) as a new sensing regime was investigated.
26404269	1	26	theme	nanocrystal-based	119:135	arg1	composites					137:146	polypyrrole-cellulose nanocrystal-based composites	97:146	polypyrrole-cellulose nanocrystal-based composites with glucose oxidase (GOx)	97:173	Integrating polypyrrole-cellulose nanocrystal-based composites with glucose oxidase (GOx) as a new sensing regime was investigated.
26404269	4	27	theme	mM	838:839	arg1	glucose					841:847	20 mM glucose	835:847	20 mM glucose	835:847	The voltammetric results showed that the native structure and biocatalytic activity of GOx immobilized on the PPy-CNC nanocomposite remained and exhibited a high sensitivity (ca. 0.73 μA·mM(-1)), with a high dynamic response ranging from 1.0 to 20 mM glucose.
26404269	3	28	theme	microscopy	428:437	arg1	images					447:452	Field emission scanning electron microscopy (FESEM) images	395:452	Field emission scanning electron microscopy (FESEM) images	395:452	Field emission scanning electron microscopy (FESEM) images showed that fibers were nanosized and porous, which is appropriate for accommodating enzymes and increasing electron transfer kinetics.
26404269	5	29	theme	interfering	951:961	arg1	cholesterol					1010:1020	cholesterol	1010:1020	cholesterol	1010:1020	The modified glucose biosensor exhibits a limit of detection (LOD) of (50 ± 10) µM and also excludes interfering species, such as ascorbic acid, uric acid, and cholesterol, which makes this sensor suitable for glucose determination in real samples.
26404269	5	29	theme	interfering	951:961	arg1	acid					1000:1003	uric acid	995:1003	uric acid	995:1003	The modified glucose biosensor exhibits a limit of detection (LOD) of (50 ± 10) µM and also excludes interfering species, such as ascorbic acid, uric acid, and cholesterol, which makes this sensor suitable for glucose determination in real samples.
26404269	5	29	theme	interfering	951:961	arg1	acid					989:992	ascorbic acid	980:992	ascorbic acid	980:992	The modified glucose biosensor exhibits a limit of detection (LOD) of (50 ± 10) µM and also excludes interfering species, such as ascorbic acid, uric acid, and cholesterol, which makes this sensor suitable for glucose determination in real samples.
26404269	5	29	theme	interfering	951:961	arg1	species					963:969	interfering species	951:969	interfering species	951:969	The modified glucose biosensor exhibits a limit of detection (LOD) of (50 ± 10) µM and also excludes interfering species, such as ascorbic acid, uric acid, and cholesterol, which makes this sensor suitable for glucose determination in real samples.
26404269	2	30	theme	physicochemical	324:338	arg1	properties					340:349	unique physicochemical properties	317:349	unique physicochemical properties	317:349	Polypyrrole-cellulose nanocrystal (PPy-CNC)-based composite as a novel immobilization membrane with unique physicochemical properties was found to enhance biosensor performance.
26404269	5	31	theme	detection	901:909	arg1	limit					892:896	a limit	890:896	a limit of detection (LOD) of (50 ± 10) µM	890:931	The modified glucose biosensor exhibits a limit of detection (LOD) of (50 ± 10) µM and also excludes interfering species, such as ascorbic acid, uric acid, and cholesterol, which makes this sensor suitable for glucose determination in real samples.
26404269	2	32	theme	biosensor	372:380	arg1	performance					382:392	biosensor performance	372:392	biosensor performance	372:392	Polypyrrole-cellulose nanocrystal (PPy-CNC)-based composite as a novel immobilization membrane with unique physicochemical properties was found to enhance biosensor performance.
26404269	2	33	theme	-based	260:265	arg1	composite					267:275	Polypyrrole-cellulose nanocrystal (PPy-CNC)-based composite	217:275	Polypyrrole-cellulose nanocrystal (PPy-CNC)-based composite as a novel immobilization membrane with unique physicochemical properties	217:349	Polypyrrole-cellulose nanocrystal (PPy-CNC)-based composite as a novel immobilization membrane with unique physicochemical properties was found to enhance biosensor performance.
26404269	5	34	theme	glucose	863:869	arg1	biosensor					871:879	The modified glucose biosensor	850:879	The modified glucose biosensor	850:879	The modified glucose biosensor exhibits a limit of detection (LOD) of (50 ± 10) µM and also excludes interfering species, such as ascorbic acid, uric acid, and cholesterol, which makes this sensor suitable for glucose determination in real samples.
26404269	0	35	theme	Cellulose	34:42	arg1	Effect					8:13	Synergy Effect	0:13	Synergy Effect of Nanocrystalline Cellulose for the Biosensing Detection of Glucose.	0:83	Synergy Effect of Nanocrystalline Cellulose for the Biosensing Detection of Glucose.
26404269	3	36	theme	electron	419:426	arg1	FESEM					440:444	FESEM	440:444	FESEM	440:444	Field emission scanning electron microscopy (FESEM) images showed that fibers were nanosized and porous, which is appropriate for accommodating enzymes and increasing electron transfer kinetics.
26404269	3	36	theme	electron	419:426	arg1	microscopy					428:437	Field emission scanning electron microscopy	395:437	Field emission scanning electron microscopy (FESEM) images	395:452	Field emission scanning electron microscopy (FESEM) images showed that fibers were nanosized and porous, which is appropriate for accommodating enzymes and increasing electron transfer kinetics.
26404269	4	37	theme	PPy-CNC	700:706	arg1	nanocomposite					708:720	the PPy-CNC nanocomposite	696:720	the PPy-CNC nanocomposite	696:720	The voltammetric results showed that the native structure and biocatalytic activity of GOx immobilized on the PPy-CNC nanocomposite remained and exhibited a high sensitivity (ca. 0.73 μA·mM(-1)), with a high dynamic response ranging from 1.0 to 20 mM glucose.
26404269	0	38	theme	Biosensing	52:61	arg1	Detection					63:71	the Biosensing Detection	48:71	the Biosensing Detection of Glucose	48:82	Synergy Effect of Nanocrystalline Cellulose for the Biosensing Detection of Glucose.
26404269	5	39	theme	uric	995:998	arg1	acid					1000:1003	uric acid	995:1003	uric acid	995:1003	The modified glucose biosensor exhibits a limit of detection (LOD) of (50 ± 10) µM and also excludes interfering species, such as ascorbic acid, uric acid, and cholesterol, which makes this sensor suitable for glucose determination in real samples.
26404269	5	40	from	determination	1068:1080	arg1	samples					1090:1096	real samples	1085:1096	real samples	1085:1096	The modified glucose biosensor exhibits a limit of detection (LOD) of (50 ± 10) µM and also excludes interfering species, such as ascorbic acid, uric acid, and cholesterol, which makes this sensor suitable for glucose determination in real samples.
26404269	7	41	theme	value	1235:1239	arg1	%					1218:1218	95%	1216:1218	95% of the initial value after 17 days	1216:1253	The current response was maintained over 95% of the initial value after 17 days, and the current difference measurement obtained using different electrodes provided a relative standard deviation (RSD) of 4.47%.
26404269	7	41	theme	value	1235:1239	arg1	value					1235:1239	the initial value	1223:1239	the initial value after 17 days	1223:1253	The current response was maintained over 95% of the initial value after 17 days, and the current difference measurement obtained using different electrodes provided a relative standard deviation (RSD) of 4.47%.
26404269	5	42	theme	µM	930:931	arg1	LOD					912:914	LOD	912:914	LOD	912:914	The modified glucose biosensor exhibits a limit of detection (LOD) of (50 ± 10) µM and also excludes interfering species, such as ascorbic acid, uric acid, and cholesterol, which makes this sensor suitable for glucose determination in real samples.
26404269	5	42	theme	µM	930:931	arg1	detection					901:909	detection	901:909	detection (LOD) of (50 ± 10) µM	901:931	The modified glucose biosensor exhibits a limit of detection (LOD) of (50 ± 10) µM and also excludes interfering species, such as ascorbic acid, uric acid, and cholesterol, which makes this sensor suitable for glucose determination in real samples.
26404269	4	43	theme	voltammetric	594:605	arg1	results					607:613	The voltammetric results	590:613	The voltammetric results	590:613	The voltammetric results showed that the native structure and biocatalytic activity of GOx immobilized on the PPy-CNC nanocomposite remained and exhibited a high sensitivity (ca. 0.73 μA·mM(-1)), with a high dynamic response ranging from 1.0 to 20 mM glucose.
26404269	1	44	theme	glucose	153:159	arg1	oxidase					161:167	glucose oxidase	153:167	glucose oxidase (GOx)	153:173	Integrating polypyrrole-cellulose nanocrystal-based composites with glucose oxidase (GOx) as a new sensing regime was investigated.
26404269	1	44	theme	glucose	153:159	arg1	GOx					170:172	GOx	170:172	GOx	170:172	Integrating polypyrrole-cellulose nanocrystal-based composites with glucose oxidase (GOx) as a new sensing regime was investigated.
26404269	7	45	theme	different	1310:1318	arg1	electrodes					1320:1329	different electrodes	1310:1329	different electrodes	1310:1329	The current response was maintained over 95% of the initial value after 17 days, and the current difference measurement obtained using different electrodes provided a relative standard deviation (RSD) of 4.47%.
26404269	2	46	theme	novel	282:286	arg1	membrane					303:310	a novel immobilization membrane	280:310	a novel immobilization membrane with unique physicochemical properties	280:349	Polypyrrole-cellulose nanocrystal (PPy-CNC)-based composite as a novel immobilization membrane with unique physicochemical properties was found to enhance biosensor performance.
26404269	4	47	theme	20	835:836	arg1	mM					838:839	mM	838:839	mM	838:839	The voltammetric results showed that the native structure and biocatalytic activity of GOx immobilized on the PPy-CNC nanocomposite remained and exhibited a high sensitivity (ca. 0.73 μA·mM(-1)), with a high dynamic response ranging from 1.0 to 20 mM glucose.
26404269	4	48	dep	μA·mM	774:778	arg1	ca.					765:767	ca.	765:767	ca.	765:767	The voltammetric results showed that the native structure and biocatalytic activity of GOx immobilized on the PPy-CNC nanocomposite remained and exhibited a high sensitivity (ca. 0.73 μA·mM(-1)), with a high dynamic response ranging from 1.0 to 20 mM glucose.
26404269	2	49	with	membrane	303:310	arg1	properties					340:349	unique physicochemical properties	317:349	unique physicochemical properties	317:349	Polypyrrole-cellulose nanocrystal (PPy-CNC)-based composite as a novel immobilization membrane with unique physicochemical properties was found to enhance biosensor performance.
26404269	4	50	theme	dynamic	798:804	arg1	response					806:813	a high dynamic response	791:813	a high dynamic response ranging from 1.0 to 20 mM glucose	791:847	The voltammetric results showed that the native structure and biocatalytic activity of GOx immobilized on the PPy-CNC nanocomposite remained and exhibited a high sensitivity (ca. 0.73 μA·mM(-1)), with a high dynamic response ranging from 1.0 to 20 mM glucose.
26404269	4	51	theme	high	793:796	arg1	response					806:813	a high dynamic response	791:813	a high dynamic response ranging from 1.0 to 20 mM glucose	791:847	The voltammetric results showed that the native structure and biocatalytic activity of GOx immobilized on the PPy-CNC nanocomposite remained and exhibited a high sensitivity (ca. 0.73 μA·mM(-1)), with a high dynamic response ranging from 1.0 to 20 mM glucose.
26404269	4	52	theme	high	747:750	arg1	sensitivity					752:762	a high sensitivity	745:762	a high sensitivity (ca. 0.73 μA·mM(-1))	745:783	The voltammetric results showed that the native structure and biocatalytic activity of GOx immobilized on the PPy-CNC nanocomposite remained and exhibited a high sensitivity (ca. 0.73 μA·mM(-1)), with a high dynamic response ranging from 1.0 to 20 mM glucose.
26404269	3	53	theme	electron	562:569	arg1	kinetics					580:587	electron transfer kinetics	562:587	electron transfer kinetics	562:587	Field emission scanning electron microscopy (FESEM) images showed that fibers were nanosized and porous, which is appropriate for accommodating enzymes and increasing electron transfer kinetics.
26404269	5	54	theme	ascorbic	980:987	arg1	acid					989:992	ascorbic acid	980:992	ascorbic acid	980:992	The modified glucose biosensor exhibits a limit of detection (LOD) of (50 ± 10) µM and also excludes interfering species, such as ascorbic acid, uric acid, and cholesterol, which makes this sensor suitable for glucose determination in real samples.
26404269	3	55	theme	transfer	571:578	arg1	kinetics					580:587	electron transfer kinetics	562:587	electron transfer kinetics	562:587	Field emission scanning electron microscopy (FESEM) images showed that fibers were nanosized and porous, which is appropriate for accommodating enzymes and increasing electron transfer kinetics.
26404269	4	56	theme	GOx	677:679	arg1	structure					638:646	the native structure	627:646	the native structure	627:646	The voltammetric results showed that the native structure and biocatalytic activity of GOx immobilized on the PPy-CNC nanocomposite remained and exhibited a high sensitivity (ca. 0.73 μA·mM(-1)), with a high dynamic response ranging from 1.0 to 20 mM glucose.
26404269	4	56	theme	GOx	677:679	arg1	activity					665:672	biocatalytic activity	652:672	biocatalytic activity	652:672	The voltammetric results showed that the native structure and biocatalytic activity of GOx immobilized on the PPy-CNC nanocomposite remained and exhibited a high sensitivity (ca. 0.73 μA·mM(-1)), with a high dynamic response ranging from 1.0 to 20 mM glucose.
25065649	4	0	contain	containing	1036:1045	arg2	A2					1066:1067	A2	1066:1067	A2	1066:1067	Mechanical testing revealed an increase in compressive modulus in samples containing S2 and NBG but not A2.
25065649	4	0	contain	containing	1036:1045	arg2	NBG					1054:1056	NBG	1054:1056	NBG	1054:1056	Mechanical testing revealed an increase in compressive modulus in samples containing S2 and NBG but not A2.
25065649	4	0	contain	containing	1036:1045	arg2	S2					1047:1048	S2	1047:1048	S2	1047:1048	Mechanical testing revealed an increase in compressive modulus in samples containing S2 and NBG but not A2.
25065649	4	0	contain	containing	1036:1045	arg1	samples					1028:1034	samples	1028:1034	samples containing S2 and NBG but not A2	1028:1067	Mechanical testing revealed an increase in compressive modulus in samples containing S2 and NBG but not A2.
25065649	6	1	theme	Cell	1297:1300	arg1	characterization					1313:1328	Cell biological characterization	1297:1328	Cell biological characterization using rat mesenchymal stem cells (rMSCs)	1297:1369	Cell biological characterization using rat mesenchymal stem cells (rMSCs) revealed a stimulatory effect of NBG on rMSC differentiation.
25065649	1	2	theme	gellan	192:197	arg1	GG					204:205	GG	204:205	GG	204:205	Hydrogels of biocompatible calcium-crosslinkable polysaccharide gellan gum (GG) were enriched with bioglass particles to enhance (i) mineralization with calcium phosphate (CaP); (ii) antibacterial properties and (iii) growth of bone-forming cells for future bone regeneration applications.
25065649	1	2	theme	gellan	192:197	arg1	gum					199:201	biocompatible calcium-crosslinkable polysaccharide gellan gum	141:201	biocompatible calcium-crosslinkable polysaccharide gellan gum (GG)	141:206	Hydrogels of biocompatible calcium-crosslinkable polysaccharide gellan gum (GG) were enriched with bioglass particles to enhance (i) mineralization with calcium phosphate (CaP); (ii) antibacterial properties and (iii) growth of bone-forming cells for future bone regeneration applications.
25065649	3	3	theme	bioglass-free	807:819	arg1	samples					821:827	bioglass-free samples	807:827	bioglass-free samples	807:827	Incubation in SBF for 7 d, 14 d and 21 d caused apatite formation in bioglass-containing but not in bioglass-free samples, as confirmed by FTIR, XRD, SEM, ICP-OES, and measurements of dry mass, i.e. mass attributable to polymer and mineral and not water.
25065649	5	4	theme	Antibacterial	1070:1082	arg1	testing					1084:1090	Antibacterial testing	1070:1090	Antibacterial testing using biofilm-forming meticillin-resistant staphylococcus aureus (MRSA)	1070:1162	Antibacterial testing using biofilm-forming meticillin-resistant staphylococcus aureus (MRSA) showed markedly higher antibacterial activity of samples containing A2 and S2 than samples containing NBG and bioglass-free samples.
25065649	0	5	theme	gum	79:81	arg1	hydrogel					83:90	gellan gum hydrogel	72:90	gellan gum hydrogel enriched with different bioglasses	72:125	Injectable self-gelling composites for bone tissue engineering based on gellan gum hydrogel enriched with different bioglasses.
25065649	1	6	theme	gum	199:201	arg1	Hydrogels					128:136	Hydrogels	128:136	Hydrogels of biocompatible calcium-crosslinkable polysaccharide gellan gum (GG)	128:206	Hydrogels of biocompatible calcium-crosslinkable polysaccharide gellan gum (GG) were enriched with bioglass particles to enhance (i) mineralization with calcium phosphate (CaP); (ii) antibacterial properties and (iii) growth of bone-forming cells for future bone regeneration applications.
25065649	5	7	theme	higher	1180:1185	arg1	activity					1201:1208	markedly higher antibacterial activity	1171:1208	markedly higher antibacterial activity of samples containing A2 and S2 than samples containing NBG and bioglass-free samples	1171:1294	Antibacterial testing using biofilm-forming meticillin-resistant staphylococcus aureus (MRSA) showed markedly higher antibacterial activity of samples containing A2 and S2 than samples containing NBG and bioglass-free samples.
25065649	2	8	dep	technique	534:542	arg1	referred					555:562	referred	555:562	referred to as A2 and S2, respectively	555:592	Three bioglasses were compared, namely one calcium-rich and one calcium-poor preparation both produced by a sol-gel technique (hereafter referred to as A2 and S2, respectively) and one preparation of composition close to that of the commonly used 45S5 type (hereafter referred to as NBG).
25065649	3	9	theme	i.e.	901:904	arg1	mass					895:898	dry mass	891:898	dry mass	891:898	Incubation in SBF for 7 d, 14 d and 21 d caused apatite formation in bioglass-containing but not in bioglass-free samples, as confirmed by FTIR, XRD, SEM, ICP-OES, and measurements of dry mass, i.e. mass attributable to polymer and mineral and not water.
25065649	3	9	theme	i.e.	901:904	arg1	mass					906:909	i.e. mass	901:909	i.e. mass attributable to polymer and mineral and not water	901:959	Incubation in SBF for 7 d, 14 d and 21 d caused apatite formation in bioglass-containing but not in bioglass-free samples, as confirmed by FTIR, XRD, SEM, ICP-OES, and measurements of dry mass, i.e. mass attributable to polymer and mineral and not water.
25065649	5	10	contain	containing	1255:1264	arg2	NBG					1266:1268	NBG	1266:1268	NBG	1266:1268	Antibacterial testing using biofilm-forming meticillin-resistant staphylococcus aureus (MRSA) showed markedly higher antibacterial activity of samples containing A2 and S2 than samples containing NBG and bioglass-free samples.
25065649	5	10	contain	containing	1255:1264	arg1	samples					1247:1253	samples	1247:1253	samples containing NBG	1247:1268	Antibacterial testing using biofilm-forming meticillin-resistant staphylococcus aureus (MRSA) showed markedly higher antibacterial activity of samples containing A2 and S2 than samples containing NBG and bioglass-free samples.
25065649	6	11	theme	stimulatory	1382:1392	arg1	effect					1394:1399	a stimulatory effect	1380:1399	a stimulatory effect of NBG on rMSC differentiation	1380:1430	Cell biological characterization using rat mesenchymal stem cells (rMSCs) revealed a stimulatory effect of NBG on rMSC differentiation.
25065649	7	12	theme	bioglass	1449:1456	arg1	addition					1437:1444	The addition	1433:1444	The addition of bioglass	1433:1456	The addition of bioglass thus promotes GG mineralizability and, depending on bioglass type, antibacterial properties and rMSC differentiation.
25065649	2	13	dep	one	457:459	arg1	both					507:510	both	507:510	both	507:510	Three bioglasses were compared, namely one calcium-rich and one calcium-poor preparation both produced by a sol-gel technique (hereafter referred to as A2 and S2, respectively) and one preparation of composition close to that of the commonly used 45S5 type (hereafter referred to as NBG).
25065649	2	14	theme	composition	618:628	arg1	technique					534:542	a sol-gel technique	524:542	a sol-gel technique (hereafter referred to as A2 and S2, respectively)	524:593	Three bioglasses were compared, namely one calcium-rich and one calcium-poor preparation both produced by a sol-gel technique (hereafter referred to as A2 and S2, respectively) and one preparation of composition close to that of the commonly used 45S5 type (hereafter referred to as NBG).
25065649	2	14	theme	composition	618:628	arg1	preparation					603:613	one preparation	599:613	one preparation of composition close to that of the commonly used 45S5 type (hereafter referred to as NBG)	599:704	Three bioglasses were compared, namely one calcium-rich and one calcium-poor preparation both produced by a sol-gel technique (hereafter referred to as A2 and S2, respectively) and one preparation of composition close to that of the commonly used 45S5 type (hereafter referred to as NBG).
25065649	2	15	dep	referred	686:693	arg1	hereafter					676:684	hereafter	676:684	hereafter	676:684	Three bioglasses were compared, namely one calcium-rich and one calcium-poor preparation both produced by a sol-gel technique (hereafter referred to as A2 and S2, respectively) and one preparation of composition close to that of the commonly used 45S5 type (hereafter referred to as NBG).
25065649	1	16	theme	calcium	281:287	arg1	CaP					300:302	CaP	300:302	CaP	300:302	Hydrogels of biocompatible calcium-crosslinkable polysaccharide gellan gum (GG) were enriched with bioglass particles to enhance (i) mineralization with calcium phosphate (CaP); (ii) antibacterial properties and (iii) growth of bone-forming cells for future bone regeneration applications.
25065649	1	16	theme	calcium	281:287	arg1	phosphate					289:297	calcium phosphate	281:297	calcium phosphate (CaP)	281:303	Hydrogels of biocompatible calcium-crosslinkable polysaccharide gellan gum (GG) were enriched with bioglass particles to enhance (i) mineralization with calcium phosphate (CaP); (ii) antibacterial properties and (iii) growth of bone-forming cells for future bone regeneration applications.
25065649	3	17	theme	mass	895:898	arg1	FTIR					846:849	FTIR	846:849	FTIR	846:849	Incubation in SBF for 7 d, 14 d and 21 d caused apatite formation in bioglass-containing but not in bioglass-free samples, as confirmed by FTIR, XRD, SEM, ICP-OES, and measurements of dry mass, i.e. mass attributable to polymer and mineral and not water.
25065649	3	17	theme	mass	895:898	arg1	ICP-OES					862:868	ICP-OES	862:868	ICP-OES	862:868	Incubation in SBF for 7 d, 14 d and 21 d caused apatite formation in bioglass-containing but not in bioglass-free samples, as confirmed by FTIR, XRD, SEM, ICP-OES, and measurements of dry mass, i.e. mass attributable to polymer and mineral and not water.
25065649	3	17	theme	mass	895:898	arg1	measurements					875:886	measurements	875:886	measurements of dry mass, i.e. mass attributable to polymer and mineral and not water	875:959	Incubation in SBF for 7 d, 14 d and 21 d caused apatite formation in bioglass-containing but not in bioglass-free samples, as confirmed by FTIR, XRD, SEM, ICP-OES, and measurements of dry mass, i.e. mass attributable to polymer and mineral and not water.
25065649	3	17	theme	mass	895:898	arg1	SEM					857:859	SEM	857:859	SEM	857:859	Incubation in SBF for 7 d, 14 d and 21 d caused apatite formation in bioglass-containing but not in bioglass-free samples, as confirmed by FTIR, XRD, SEM, ICP-OES, and measurements of dry mass, i.e. mass attributable to polymer and mineral and not water.
25065649	3	17	theme	mass	895:898	arg1	XRD					852:854	XRD	852:854	XRD	852:854	Incubation in SBF for 7 d, 14 d and 21 d caused apatite formation in bioglass-containing but not in bioglass-free samples, as confirmed by FTIR, XRD, SEM, ICP-OES, and measurements of dry mass, i.e. mass attributable to polymer and mineral and not water.
25065649	6	18	theme	mesenchymal	1340:1350	arg1	rMSCs					1364:1368	rMSCs	1364:1368	rMSCs	1364:1368	Cell biological characterization using rat mesenchymal stem cells (rMSCs) revealed a stimulatory effect of NBG on rMSC differentiation.
25065649	6	18	theme	mesenchymal	1340:1350	arg1	cells					1357:1361	rat mesenchymal stem cells	1336:1361	rat mesenchymal stem cells (rMSCs)	1336:1369	Cell biological characterization using rat mesenchymal stem cells (rMSCs) revealed a stimulatory effect of NBG on rMSC differentiation.
25065649	4	19	theme	compressive	1005:1015	arg1	modulus					1017:1023	compressive modulus	1005:1023	compressive modulus in samples containing S2 and NBG but not A2	1005:1067	Mechanical testing revealed an increase in compressive modulus in samples containing S2 and NBG but not A2.
25065649	0	20	theme	different	106:114	arg1	bioglasses					116:125	different bioglasses	106:125	different bioglasses	106:125	Injectable self-gelling composites for bone tissue engineering based on gellan gum hydrogel enriched with different bioglasses.
25065649	6	21	theme	stem	1352:1355	arg1	rMSCs					1364:1368	rMSCs	1364:1368	rMSCs	1364:1368	Cell biological characterization using rat mesenchymal stem cells (rMSCs) revealed a stimulatory effect of NBG on rMSC differentiation.
25065649	6	21	theme	stem	1352:1355	arg1	cells					1357:1361	rat mesenchymal stem cells	1336:1361	rat mesenchymal stem cells (rMSCs)	1336:1369	Cell biological characterization using rat mesenchymal stem cells (rMSCs) revealed a stimulatory effect of NBG on rMSC differentiation.
25065649	0	22	theme	self-gelling	11:22	arg1	composites					24:33	Injectable self-gelling composites	0:33	Injectable self-gelling composites for bone tissue engineering	0:61	Injectable self-gelling composites for bone tissue engineering based on gellan gum hydrogel enriched with different bioglasses.
25065649	6	23	theme	biological	1302:1311	arg1	characterization					1313:1328	Cell biological characterization	1297:1328	Cell biological characterization using rat mesenchymal stem cells (rMSCs)	1297:1369	Cell biological characterization using rat mesenchymal stem cells (rMSCs) revealed a stimulatory effect of NBG on rMSC differentiation.
25065649	4	24	from	modulus	1017:1023	arg1	samples					1028:1034	samples	1028:1034	samples containing S2 and NBG but not A2	1028:1067	Mechanical testing revealed an increase in compressive modulus in samples containing S2 and NBG but not A2.
25065649	0	25	theme	Injectable	0:9	arg1	composites					24:33	Injectable self-gelling composites	0:33	Injectable self-gelling composites for bone tissue engineering	0:61	Injectable self-gelling composites for bone tissue engineering based on gellan gum hydrogel enriched with different bioglasses.
25065649	2	26	dep	referred	555:562	arg1	hereafter					545:553	hereafter	545:553	hereafter	545:553	Three bioglasses were compared, namely one calcium-rich and one calcium-poor preparation both produced by a sol-gel technique (hereafter referred to as A2 and S2, respectively) and one preparation of composition close to that of the commonly used 45S5 type (hereafter referred to as NBG).
25065649	3	27	from	Incubation	707:716	arg1	SBF					721:723	SBF	721:723	SBF	721:723	Incubation in SBF for 7 d, 14 d and 21 d caused apatite formation in bioglass-containing but not in bioglass-free samples, as confirmed by FTIR, XRD, SEM, ICP-OES, and measurements of dry mass, i.e. mass attributable to polymer and mineral and not water.
25065649	5	28	theme	samples	1213:1219	arg1	activity					1201:1208	markedly higher antibacterial activity	1171:1208	markedly higher antibacterial activity of samples containing A2 and S2 than samples containing NBG and bioglass-free samples	1171:1294	Antibacterial testing using biofilm-forming meticillin-resistant staphylococcus aureus (MRSA) showed markedly higher antibacterial activity of samples containing A2 and S2 than samples containing NBG and bioglass-free samples.
25065649	6	29	from	effect	1394:1399	arg1	differentiation					1416:1430	rMSC differentiation	1411:1430	rMSC differentiation	1411:1430	Cell biological characterization using rat mesenchymal stem cells (rMSCs) revealed a stimulatory effect of NBG on rMSC differentiation.
25065649	3	30	theme	attributable	911:922	arg1	mass					895:898	dry mass	891:898	dry mass	891:898	Incubation in SBF for 7 d, 14 d and 21 d caused apatite formation in bioglass-containing but not in bioglass-free samples, as confirmed by FTIR, XRD, SEM, ICP-OES, and measurements of dry mass, i.e. mass attributable to polymer and mineral and not water.
25065649	3	30	theme	attributable	911:922	arg1	mass					906:909	i.e. mass	901:909	i.e. mass attributable to polymer and mineral and not water	901:959	Incubation in SBF for 7 d, 14 d and 21 d caused apatite formation in bioglass-containing but not in bioglass-free samples, as confirmed by FTIR, XRD, SEM, ICP-OES, and measurements of dry mass, i.e. mass attributable to polymer and mineral and not water.
25065649	5	31	contain	containing	1221:1230	arg1	samples					1213:1219	samples	1213:1219	samples containing A2 and S2 than samples containing NBG and bioglass-free samples	1213:1294	Antibacterial testing using biofilm-forming meticillin-resistant staphylococcus aureus (MRSA) showed markedly higher antibacterial activity of samples containing A2 and S2 than samples containing NBG and bioglass-free samples.
25065649	5	31	contain	containing	1221:1230	arg2	S2					1239:1240	S2	1239:1240	S2	1239:1240	Antibacterial testing using biofilm-forming meticillin-resistant staphylococcus aureus (MRSA) showed markedly higher antibacterial activity of samples containing A2 and S2 than samples containing NBG and bioglass-free samples.
25065649	5	31	contain	containing	1221:1230	arg2	A2					1232:1233	A2	1232:1233	A2	1232:1233	Antibacterial testing using biofilm-forming meticillin-resistant staphylococcus aureus (MRSA) showed markedly higher antibacterial activity of samples containing A2 and S2 than samples containing NBG and bioglass-free samples.
25065649	7	32	theme	rMSC	1554:1557	arg1	differentiation					1559:1573	rMSC differentiation	1554:1573	rMSC differentiation	1554:1573	The addition of bioglass thus promotes GG mineralizability and, depending on bioglass type, antibacterial properties and rMSC differentiation.
25065649	1	33	theme	bone-forming	356:367	arg1	cells					369:373	bone-forming cells	356:373	bone-forming cells	356:373	Hydrogels of biocompatible calcium-crosslinkable polysaccharide gellan gum (GG) were enriched with bioglass particles to enhance (i) mineralization with calcium phosphate (CaP); (ii) antibacterial properties and (iii) growth of bone-forming cells for future bone regeneration applications.
25065649	6	34	theme	rMSC	1411:1414	arg1	differentiation					1416:1430	rMSC differentiation	1411:1430	rMSC differentiation	1411:1430	Cell biological characterization using rat mesenchymal stem cells (rMSCs) revealed a stimulatory effect of NBG on rMSC differentiation.
25065649	4	35	from	increase	993:1000	arg1	modulus					1017:1023	compressive modulus	1005:1023	compressive modulus in samples containing S2 and NBG but not A2	1005:1067	Mechanical testing revealed an increase in compressive modulus in samples containing S2 and NBG but not A2.
25065649	0	36	theme	tissue	44:49	arg1	engineering					51:61	bone tissue engineering	39:61	bone tissue engineering	39:61	Injectable self-gelling composites for bone tissue engineering based on gellan gum hydrogel enriched with different bioglasses.
25065649	1	37	dep	properties	325:334	arg1	ii					307:308	ii	307:308	ii	307:308	Hydrogels of biocompatible calcium-crosslinkable polysaccharide gellan gum (GG) were enriched with bioglass particles to enhance (i) mineralization with calcium phosphate (CaP); (ii) antibacterial properties and (iii) growth of bone-forming cells for future bone regeneration applications.
25065649	1	38	theme	cells	369:373	arg1	growth					346:351	(iii) growth	340:351	(iii) growth	340:351	Hydrogels of biocompatible calcium-crosslinkable polysaccharide gellan gum (GG) were enriched with bioglass particles to enhance (i) mineralization with calcium phosphate (CaP); (ii) antibacterial properties and (iii) growth of bone-forming cells for future bone regeneration applications.
25065649	1	38	theme	cells	369:373	arg1	properties					325:334	antibacterial properties	311:334	antibacterial properties	311:334	Hydrogels of biocompatible calcium-crosslinkable polysaccharide gellan gum (GG) were enriched with bioglass particles to enhance (i) mineralization with calcium phosphate (CaP); (ii) antibacterial properties and (iii) growth of bone-forming cells for future bone regeneration applications.
25065649	2	39	dep	type	670:673	arg1	referred					686:693	referred	686:693	referred to as NBG	686:703	Three bioglasses were compared, namely one calcium-rich and one calcium-poor preparation both produced by a sol-gel technique (hereafter referred to as A2 and S2, respectively) and one preparation of composition close to that of the commonly used 45S5 type (hereafter referred to as NBG).
25065649	1	40	theme	bioglass	227:234	arg1	particles					236:244	bioglass particles	227:244	bioglass particles	227:244	Hydrogels of biocompatible calcium-crosslinkable polysaccharide gellan gum (GG) were enriched with bioglass particles to enhance (i) mineralization with calcium phosphate (CaP); (ii) antibacterial properties and (iii) growth of bone-forming cells for future bone regeneration applications.
25065649	0	41	theme	bone	39:42	arg1	engineering					51:61	bone tissue engineering	39:61	bone tissue engineering	39:61	Injectable self-gelling composites for bone tissue engineering based on gellan gum hydrogel enriched with different bioglasses.
25065649	1	42	dep	growth	346:351	arg1	iii					341:343	iii	341:343	iii	341:343	Hydrogels of biocompatible calcium-crosslinkable polysaccharide gellan gum (GG) were enriched with bioglass particles to enhance (i) mineralization with calcium phosphate (CaP); (ii) antibacterial properties and (iii) growth of bone-forming cells for future bone regeneration applications.
25065649	2	43	theme	sol-gel	526:532	arg1	technique					534:542	a sol-gel technique	524:542	a sol-gel technique (hereafter referred to as A2 and S2, respectively)	524:593	Three bioglasses were compared, namely one calcium-rich and one calcium-poor preparation both produced by a sol-gel technique (hereafter referred to as A2 and S2, respectively) and one preparation of composition close to that of the commonly used 45S5 type (hereafter referred to as NBG).
25065649	6	44	theme	rat	1336:1338	arg1	rMSCs					1364:1368	rMSCs	1364:1368	rMSCs	1364:1368	Cell biological characterization using rat mesenchymal stem cells (rMSCs) revealed a stimulatory effect of NBG on rMSC differentiation.
25065649	6	44	theme	rat	1336:1338	arg1	cells					1357:1361	rat mesenchymal stem cells	1336:1361	rat mesenchymal stem cells (rMSCs)	1336:1369	Cell biological characterization using rat mesenchymal stem cells (rMSCs) revealed a stimulatory effect of NBG on rMSC differentiation.
25065649	2	45	dep	one	478:480	arg1	preparation					495:505	calcium-poor preparation	482:505	one calcium-poor preparation	478:505	Three bioglasses were compared, namely one calcium-rich and one calcium-poor preparation both produced by a sol-gel technique (hereafter referred to as A2 and S2, respectively) and one preparation of composition close to that of the commonly used 45S5 type (hereafter referred to as NBG).
25065649	1	46	theme	future	379:384	arg1	applications					404:415	future bone regeneration applications	379:415	future bone regeneration applications	379:415	Hydrogels of biocompatible calcium-crosslinkable polysaccharide gellan gum (GG) were enriched with bioglass particles to enhance (i) mineralization with calcium phosphate (CaP); (ii) antibacterial properties and (iii) growth of bone-forming cells for future bone regeneration applications.
25065649	7	47	theme	GG	1472:1473	arg1	mineralizability					1475:1490	GG mineralizability	1472:1490	GG mineralizability	1472:1490	The addition of bioglass thus promotes GG mineralizability and, depending on bioglass type, antibacterial properties and rMSC differentiation.
25065649	1	48	theme	bone	386:389	arg1	applications					404:415	future bone regeneration applications	379:415	future bone regeneration applications	379:415	Hydrogels of biocompatible calcium-crosslinkable polysaccharide gellan gum (GG) were enriched with bioglass particles to enhance (i) mineralization with calcium phosphate (CaP); (ii) antibacterial properties and (iii) growth of bone-forming cells for future bone regeneration applications.
25065649	6	49	theme	NBG	1404:1406	arg1	effect					1394:1399	a stimulatory effect	1380:1399	a stimulatory effect of NBG on rMSC differentiation	1380:1430	Cell biological characterization using rat mesenchymal stem cells (rMSCs) revealed a stimulatory effect of NBG on rMSC differentiation.
25065649	2	50	theme	45S5	665:668	arg1	type					670:673	the commonly used 45S5 type	647:673	the commonly used 45S5 type (hereafter referred to as NBG)	647:704	Three bioglasses were compared, namely one calcium-rich and one calcium-poor preparation both produced by a sol-gel technique (hereafter referred to as A2 and S2, respectively) and one preparation of composition close to that of the commonly used 45S5 type (hereafter referred to as NBG).
25065649	3	51	theme	dry	891:893	arg1	mass					895:898	dry mass	891:898	dry mass	891:898	Incubation in SBF for 7 d, 14 d and 21 d caused apatite formation in bioglass-containing but not in bioglass-free samples, as confirmed by FTIR, XRD, SEM, ICP-OES, and measurements of dry mass, i.e. mass attributable to polymer and mineral and not water.
25065649	3	51	theme	dry	891:893	arg1	mass					906:909	i.e. mass	901:909	i.e. mass attributable to polymer and mineral and not water	901:959	Incubation in SBF for 7 d, 14 d and 21 d caused apatite formation in bioglass-containing but not in bioglass-free samples, as confirmed by FTIR, XRD, SEM, ICP-OES, and measurements of dry mass, i.e. mass attributable to polymer and mineral and not water.
25065649	7	52	theme	antibacterial	1525:1537	arg1	properties					1539:1548	antibacterial properties	1525:1548	antibacterial properties	1525:1548	The addition of bioglass thus promotes GG mineralizability and, depending on bioglass type, antibacterial properties and rMSC differentiation.
25065649	1	53	theme	regeneration	391:402	arg1	applications					404:415	future bone regeneration applications	379:415	future bone regeneration applications	379:415	Hydrogels of biocompatible calcium-crosslinkable polysaccharide gellan gum (GG) were enriched with bioglass particles to enhance (i) mineralization with calcium phosphate (CaP); (ii) antibacterial properties and (iii) growth of bone-forming cells for future bone regeneration applications.
25065649	1	54	with	mineralization	261:274	arg1	CaP					300:302	CaP	300:302	CaP	300:302	Hydrogels of biocompatible calcium-crosslinkable polysaccharide gellan gum (GG) were enriched with bioglass particles to enhance (i) mineralization with calcium phosphate (CaP); (ii) antibacterial properties and (iii) growth of bone-forming cells for future bone regeneration applications.
25065649	1	54	with	mineralization	261:274	arg1	phosphate					289:297	calcium phosphate	281:297	calcium phosphate (CaP)	281:303	Hydrogels of biocompatible calcium-crosslinkable polysaccharide gellan gum (GG) were enriched with bioglass particles to enhance (i) mineralization with calcium phosphate (CaP); (ii) antibacterial properties and (iii) growth of bone-forming cells for future bone regeneration applications.
25065649	5	55	theme	antibacterial	1187:1199	arg1	activity					1201:1208	markedly higher antibacterial activity	1171:1208	markedly higher antibacterial activity of samples containing A2 and S2 than samples containing NBG and bioglass-free samples	1171:1294	Antibacterial testing using biofilm-forming meticillin-resistant staphylococcus aureus (MRSA) showed markedly higher antibacterial activity of samples containing A2 and S2 than samples containing NBG and bioglass-free samples.
25065649	7	56	theme	bioglass	1510:1517	arg1	type					1519:1522	bioglass type	1510:1522	bioglass type	1510:1522	The addition of bioglass thus promotes GG mineralizability and, depending on bioglass type, antibacterial properties and rMSC differentiation.
25065649	2	57	theme	used	660:663	arg1	type					670:673	the commonly used 45S5 type	647:673	the commonly used 45S5 type (hereafter referred to as NBG)	647:704	Three bioglasses were compared, namely one calcium-rich and one calcium-poor preparation both produced by a sol-gel technique (hereafter referred to as A2 and S2, respectively) and one preparation of composition close to that of the commonly used 45S5 type (hereafter referred to as NBG).
25065649	1	58	theme	biocompatible	141:153	arg1	GG					204:205	GG	204:205	GG	204:205	Hydrogels of biocompatible calcium-crosslinkable polysaccharide gellan gum (GG) were enriched with bioglass particles to enhance (i) mineralization with calcium phosphate (CaP); (ii) antibacterial properties and (iii) growth of bone-forming cells for future bone regeneration applications.
25065649	1	58	theme	biocompatible	141:153	arg1	gum					199:201	biocompatible calcium-crosslinkable polysaccharide gellan gum	141:201	biocompatible calcium-crosslinkable polysaccharide gellan gum (GG)	141:206	Hydrogels of biocompatible calcium-crosslinkable polysaccharide gellan gum (GG) were enriched with bioglass particles to enhance (i) mineralization with calcium phosphate (CaP); (ii) antibacterial properties and (iii) growth of bone-forming cells for future bone regeneration applications.
25065649	3	59	theme	apatite	755:761	arg1	formation					763:771	apatite formation	755:771	apatite formation	755:771	Incubation in SBF for 7 d, 14 d and 21 d caused apatite formation in bioglass-containing but not in bioglass-free samples, as confirmed by FTIR, XRD, SEM, ICP-OES, and measurements of dry mass, i.e. mass attributable to polymer and mineral and not water.
25065649	0	60	theme	gellan	72:77	arg1	hydrogel					83:90	gellan gum hydrogel	72:90	gellan gum hydrogel enriched with different bioglasses	72:125	Injectable self-gelling composites for bone tissue engineering based on gellan gum hydrogel enriched with different bioglasses.
25065649	1	61	dep	mineralization	261:274	arg1	i					258:258	i	258:258	i	258:258	Hydrogels of biocompatible calcium-crosslinkable polysaccharide gellan gum (GG) were enriched with bioglass particles to enhance (i) mineralization with calcium phosphate (CaP); (ii) antibacterial properties and (iii) growth of bone-forming cells for future bone regeneration applications.
25065649	1	62	dep	enriched	213:220	arg1	growth					346:351	(iii) growth	340:351	(iii) growth	340:351	Hydrogels of biocompatible calcium-crosslinkable polysaccharide gellan gum (GG) were enriched with bioglass particles to enhance (i) mineralization with calcium phosphate (CaP); (ii) antibacterial properties and (iii) growth of bone-forming cells for future bone regeneration applications.
25065649	1	62	dep	enriched	213:220	arg1	properties					325:334	antibacterial properties	311:334	antibacterial properties	311:334	Hydrogels of biocompatible calcium-crosslinkable polysaccharide gellan gum (GG) were enriched with bioglass particles to enhance (i) mineralization with calcium phosphate (CaP); (ii) antibacterial properties and (iii) growth of bone-forming cells for future bone regeneration applications.
25065649	1	63	theme	calcium-crosslinkable	155:175	arg1	GG					204:205	GG	204:205	GG	204:205	Hydrogels of biocompatible calcium-crosslinkable polysaccharide gellan gum (GG) were enriched with bioglass particles to enhance (i) mineralization with calcium phosphate (CaP); (ii) antibacterial properties and (iii) growth of bone-forming cells for future bone regeneration applications.
25065649	1	63	theme	calcium-crosslinkable	155:175	arg1	gum					199:201	biocompatible calcium-crosslinkable polysaccharide gellan gum	141:201	biocompatible calcium-crosslinkable polysaccharide gellan gum (GG)	141:206	Hydrogels of biocompatible calcium-crosslinkable polysaccharide gellan gum (GG) were enriched with bioglass particles to enhance (i) mineralization with calcium phosphate (CaP); (ii) antibacterial properties and (iii) growth of bone-forming cells for future bone regeneration applications.
25065649	4	64	theme	Mechanical	962:971	arg1	testing					973:979	Mechanical testing	962:979	Mechanical testing	962:979	Mechanical testing revealed an increase in compressive modulus in samples containing S2 and NBG but not A2.
25065649	3	65	theme	d	731:731	arg1	d					737:737	7 d, 14 d and 21 d	729:746	d	737:737	Incubation in SBF for 7 d, 14 d and 21 d caused apatite formation in bioglass-containing but not in bioglass-free samples, as confirmed by FTIR, XRD, SEM, ICP-OES, and measurements of dry mass, i.e. mass attributable to polymer and mineral and not water.
25065649	2	66	theme	calcium-poor	482:493	arg1	preparation					495:505	calcium-poor preparation	482:505	one calcium-poor preparation	478:505	Three bioglasses were compared, namely one calcium-rich and one calcium-poor preparation both produced by a sol-gel technique (hereafter referred to as A2 and S2, respectively) and one preparation of composition close to that of the commonly used 45S5 type (hereafter referred to as NBG).
25065649	1	67	theme	antibacterial	311:323	arg1	properties					325:334	antibacterial properties	311:334	antibacterial properties	311:334	Hydrogels of biocompatible calcium-crosslinkable polysaccharide gellan gum (GG) were enriched with bioglass particles to enhance (i) mineralization with calcium phosphate (CaP); (ii) antibacterial properties and (iii) growth of bone-forming cells for future bone regeneration applications.
25065649	5	68	theme	bioglass-free	1274:1286	arg1	samples					1288:1294	bioglass-free samples	1274:1294	bioglass-free samples	1274:1294	Antibacterial testing using biofilm-forming meticillin-resistant staphylococcus aureus (MRSA) showed markedly higher antibacterial activity of samples containing A2 and S2 than samples containing NBG and bioglass-free samples.
25065649	1	69	theme	polysaccharide	177:190	arg1	GG					204:205	GG	204:205	GG	204:205	Hydrogels of biocompatible calcium-crosslinkable polysaccharide gellan gum (GG) were enriched with bioglass particles to enhance (i) mineralization with calcium phosphate (CaP); (ii) antibacterial properties and (iii) growth of bone-forming cells for future bone regeneration applications.
25065649	1	69	theme	polysaccharide	177:190	arg1	gum					199:201	biocompatible calcium-crosslinkable polysaccharide gellan gum	141:201	biocompatible calcium-crosslinkable polysaccharide gellan gum (GG)	141:206	Hydrogels of biocompatible calcium-crosslinkable polysaccharide gellan gum (GG) were enriched with bioglass particles to enhance (i) mineralization with calcium phosphate (CaP); (ii) antibacterial properties and (iii) growth of bone-forming cells for future bone regeneration applications.
26627983	5	0	theme	final	775:779	arg1	step					781:784	The final step	771:784	The final step	771:784	The final step was accompanied by the most significant reduction in the yield of alcohol-insoluble solids as well as in the composition of uronic acids and neutral sugars; pectinases increased their activity and depolymerization of hemicellulosic fractions occurred.
26627983	1	1	theme	noni	149:152	arg1	fruit					154:158	noni fruit	149:158	noni fruit harvested at five ripening stages	149:192	Quality and compositional changes were determined in noni fruit harvested at five ripening stages, from dark-green to thaslucent-grayish.
26627983	0	2	theme	Fruit	80:84	arg1	Ripening					86:93	Noni (Morinda citrifolia L.) Fruit Ripening	51:93	Noni (Morinda citrifolia L.) Fruit Ripening	51:93	Biochemistry and Cell Wall Changes Associated with Noni (Morinda citrifolia L.) Fruit Ripening.
26627983	2	3	theme	softening	339:347	arg1	profile					349:355	the softening profile	335:355	the softening profile	335:355	Fruit ripening was accompanied by acidity and soluble solids accumulation but pH diminution, whereas the softening profile presented three differential steps named early (no significant softening), intermediate (significant softening), and final (dramatic softening).
26627983	2	4	theme	soluble	280:286	arg1	solids					288:293	soluble solids	280:293	soluble solids	280:293	Fruit ripening was accompanied by acidity and soluble solids accumulation but pH diminution, whereas the softening profile presented three differential steps named early (no significant softening), intermediate (significant softening), and final (dramatic softening).
26627983	4	5	theme	hemicellulases	744:757	arg1	increment					716:724	an increment	713:724	an increment of pectinases and hemicellulases activities	713:768	The intermediate step showed an increment of pectinases and hemicellulases activities.
26627983	3	6	theme	hydrosoluble	550:561	arg1	pectins					563:569	hydrosoluble pectins	550:569	hydrosoluble pectins	550:569	At early step the extensive depolymerization of hydrosoluble pectins and the significantly increment of pectinase activities did not correlate with the slight reduction in firmness.
26627983	5	7	from	reduction	826:834	arg1	composition					895:905	the composition	891:905	the composition of uronic acids and neutral sugars	891:940	The final step was accompanied by the most significant reduction in the yield of alcohol-insoluble solids as well as in the composition of uronic acids and neutral sugars; pectinases increased their activity and depolymerization of hemicellulosic fractions occurred.
26627983	5	7	from	reduction	826:834	arg1	yield					843:847	the yield	839:847	the yield of alcohol-insoluble solids	839:875	The final step was accompanied by the most significant reduction in the yield of alcohol-insoluble solids as well as in the composition of uronic acids and neutral sugars; pectinases increased their activity and depolymerization of hemicellulosic fractions occurred.
26627983	3	8	theme	extensive	520:528	arg1	depolymerization					530:545	the extensive depolymerization	516:545	the extensive depolymerization of hydrosoluble pectins	516:569	At early step the extensive depolymerization of hydrosoluble pectins and the significantly increment of pectinase activities did not correlate with the slight reduction in firmness.
26627983	5	9	theme	neutral	927:933	arg1	sugars					935:940	neutral sugars	927:940	neutral sugars	927:940	The final step was accompanied by the most significant reduction in the yield of alcohol-insoluble solids as well as in the composition of uronic acids and neutral sugars; pectinases increased their activity and depolymerization of hemicellulosic fractions occurred.
26627983	4	10	theme	intermediate	688:699	arg1	step					701:704	The intermediate step	684:704	The intermediate step	684:704	The intermediate step showed an increment of pectinases and hemicellulases activities.
26627983	2	11	theme	acidity	268:274	arg1	accumulation					295:306	acidity and soluble solids accumulation	268:306	acidity and soluble solids accumulation	268:306	Fruit ripening was accompanied by acidity and soluble solids accumulation but pH diminution, whereas the softening profile presented three differential steps named early (no significant softening), intermediate (significant softening), and final (dramatic softening).
26627983	5	12	theme	solids	870:875	arg1	yield					843:847	the yield	839:847	the yield of alcohol-insoluble solids	839:875	The final step was accompanied by the most significant reduction in the yield of alcohol-insoluble solids as well as in the composition of uronic acids and neutral sugars; pectinases increased their activity and depolymerization of hemicellulosic fractions occurred.
26627983	5	13	theme	sugars	935:940	arg1	composition					895:905	the composition	891:905	the composition of uronic acids and neutral sugars	891:940	The final step was accompanied by the most significant reduction in the yield of alcohol-insoluble solids as well as in the composition of uronic acids and neutral sugars; pectinases increased their activity and depolymerization of hemicellulosic fractions occurred.
26627983	6	14	theme	pectinases	1104:1113	arg1	action					1094:1099	the coordinated action	1078:1099	the coordinated action of pectinases and hemicellulases that promote the differential dissasembly of cell wall polymers	1078:1196	Noni ripening is a process conducted by the coordinated action of pectinases and hemicellulases that promote the differential dissasembly of cell wall polymers.
26627983	6	15	theme	polymers	1189:1196	arg1	dissasembly					1164:1174	the differential dissasembly	1147:1174	the differential dissasembly of cell wall polymers	1147:1196	Noni ripening is a process conducted by the coordinated action of pectinases and hemicellulases that promote the differential dissasembly of cell wall polymers.
26627983	5	16	theme	fractions	1018:1026	arg1	depolymerization					983:998	depolymerization	983:998	depolymerization of hemicellulosic fractions	983:1026	The final step was accompanied by the most significant reduction in the yield of alcohol-insoluble solids as well as in the composition of uronic acids and neutral sugars; pectinases increased their activity and depolymerization of hemicellulosic fractions occurred.
26627983	2	17	theme	pH	312:313	arg1	diminution					315:324	pH diminution	312:324	pH diminution	312:324	Fruit ripening was accompanied by acidity and soluble solids accumulation but pH diminution, whereas the softening profile presented three differential steps named early (no significant softening), intermediate (significant softening), and final (dramatic softening).
26627983	6	18	theme	coordinated	1082:1092	arg1	action					1094:1099	the coordinated action	1078:1099	the coordinated action of pectinases and hemicellulases that promote the differential dissasembly of cell wall polymers	1078:1196	Noni ripening is a process conducted by the coordinated action of pectinases and hemicellulases that promote the differential dissasembly of cell wall polymers.
26627983	5	19	theme	significant	814:824	arg1	reduction					826:834	the most significant reduction	805:834	the most significant reduction in the yield of alcohol-insoluble solids as well as in the composition of uronic acids and neutral sugars	805:940	The final step was accompanied by the most significant reduction in the yield of alcohol-insoluble solids as well as in the composition of uronic acids and neutral sugars; pectinases increased their activity and depolymerization of hemicellulosic fractions occurred.
26627983	2	20	dep	intermediate	432:443	arg1	softening					458:466	significant softening	446:466	significant softening	446:466	Fruit ripening was accompanied by acidity and soluble solids accumulation but pH diminution, whereas the softening profile presented three differential steps named early (no significant softening), intermediate (significant softening), and final (dramatic softening).
26627983	3	21	theme	pectins	563:569	arg1	increment					593:601	the significantly increment	575:601	the significantly increment of pectinase activities	575:625	At early step the extensive depolymerization of hydrosoluble pectins and the significantly increment of pectinase activities did not correlate with the slight reduction in firmness.
26627983	3	21	theme	pectins	563:569	arg1	depolymerization					530:545	the extensive depolymerization	516:545	the extensive depolymerization of hydrosoluble pectins	516:569	At early step the extensive depolymerization of hydrosoluble pectins and the significantly increment of pectinase activities did not correlate with the slight reduction in firmness.
26627983	2	22	theme	differential	373:384	arg1	steps					386:390	three differential steps	367:390	three differential steps named early (no significant softening), intermediate (significant softening), and final (dramatic softening)	367:499	Fruit ripening was accompanied by acidity and soluble solids accumulation but pH diminution, whereas the softening profile presented three differential steps named early (no significant softening), intermediate (significant softening), and final (dramatic softening).
26627983	0	23	theme	Wall	22:25	arg1	Changes					27:33	Cell Wall Changes	17:33	Cell Wall Changes	17:33	Biochemistry and Cell Wall Changes Associated with Noni (Morinda citrifolia L.) Fruit Ripening.
26627983	4	24	theme	pectinases	729:738	arg1	increment					716:724	an increment	713:724	an increment of pectinases and hemicellulases activities	713:768	The intermediate step showed an increment of pectinases and hemicellulases activities.
26627983	0	25	dep	Morinda	57:63	arg1	L.					76:77	Morinda citrifolia L.	57:77	Morinda citrifolia L.	57:77	Biochemistry and Cell Wall Changes Associated with Noni (Morinda citrifolia L.) Fruit Ripening.
26627983	1	26	theme	ripening	178:185	arg1	stages					187:192	five ripening stages	173:192	five ripening stages	173:192	Quality and compositional changes were determined in noni fruit harvested at five ripening stages, from dark-green to thaslucent-grayish.
26627983	0	27	theme	Cell	17:20	arg1	Changes					27:33	Cell Wall Changes	17:33	Cell Wall Changes	17:33	Biochemistry and Cell Wall Changes Associated with Noni (Morinda citrifolia L.) Fruit Ripening.
26627983	3	28	theme	early	505:509	arg1	step					511:514	early step	505:514	early step	505:514	At early step the extensive depolymerization of hydrosoluble pectins and the significantly increment of pectinase activities did not correlate with the slight reduction in firmness.
26627983	2	29	theme	solids	288:293	arg1	accumulation					295:306	acidity and soluble solids accumulation	268:306	acidity and soluble solids accumulation	268:306	Fruit ripening was accompanied by acidity and soluble solids accumulation but pH diminution, whereas the softening profile presented three differential steps named early (no significant softening), intermediate (significant softening), and final (dramatic softening).
26627983	2	30	theme	significant	408:418	arg1	softening					420:428	no significant softening	405:428	no significant softening	405:428	Fruit ripening was accompanied by acidity and soluble solids accumulation but pH diminution, whereas the softening profile presented three differential steps named early (no significant softening), intermediate (significant softening), and final (dramatic softening).
26627983	6	31	theme	differential	1151:1162	arg1	dissasembly					1164:1174	the differential dissasembly	1147:1174	the differential dissasembly of cell wall polymers	1147:1196	Noni ripening is a process conducted by the coordinated action of pectinases and hemicellulases that promote the differential dissasembly of cell wall polymers.
26627983	3	32	theme	significantly	579:591	arg1	increment					593:601	the significantly increment	575:601	the significantly increment of pectinase activities	575:625	At early step the extensive depolymerization of hydrosoluble pectins and the significantly increment of pectinase activities did not correlate with the slight reduction in firmness.
26627983	6	33	theme	Noni	1038:1041	arg1	ripening					1043:1050	Noni ripening	1038:1050	Noni ripening	1038:1050	Noni ripening is a process conducted by the coordinated action of pectinases and hemicellulases that promote the differential dissasembly of cell wall polymers.
26627983	6	33	theme	Noni	1038:1041	arg1	process					1057:1063	a process	1055:1063	a process conducted by the coordinated action of pectinases and hemicellulases that promote the differential dissasembly of cell wall polymers	1055:1196	Noni ripening is a process conducted by the coordinated action of pectinases and hemicellulases that promote the differential dissasembly of cell wall polymers.
26627983	5	34	theme	alcohol-insoluble	852:868	arg1	solids					870:875	alcohol-insoluble solids	852:875	alcohol-insoluble solids	852:875	The final step was accompanied by the most significant reduction in the yield of alcohol-insoluble solids as well as in the composition of uronic acids and neutral sugars; pectinases increased their activity and depolymerization of hemicellulosic fractions occurred.
26627983	0	35	theme	Noni	51:54	arg1	Ripening					86:93	Noni (Morinda citrifolia L.) Fruit Ripening	51:93	Noni (Morinda citrifolia L.) Fruit Ripening	51:93	Biochemistry and Cell Wall Changes Associated with Noni (Morinda citrifolia L.) Fruit Ripening.
26627983	5	36	theme	uronic	910:915	arg1	acids					917:921	uronic acids	910:921	uronic acids	910:921	The final step was accompanied by the most significant reduction in the yield of alcohol-insoluble solids as well as in the composition of uronic acids and neutral sugars; pectinases increased their activity and depolymerization of hemicellulosic fractions occurred.
26627983	2	37	theme	significant	446:456	arg1	softening					458:466	significant softening	446:466	significant softening	446:466	Fruit ripening was accompanied by acidity and soluble solids accumulation but pH diminution, whereas the softening profile presented three differential steps named early (no significant softening), intermediate (significant softening), and final (dramatic softening).
26627983	5	38	theme	acids	917:921	arg1	composition					895:905	the composition	891:905	the composition of uronic acids and neutral sugars	891:940	The final step was accompanied by the most significant reduction in the yield of alcohol-insoluble solids as well as in the composition of uronic acids and neutral sugars; pectinases increased their activity and depolymerization of hemicellulosic fractions occurred.
26627983	2	39	theme	Fruit	234:238	arg1	ripening					240:247	Fruit ripening	234:247	Fruit ripening	234:247	Fruit ripening was accompanied by acidity and soluble solids accumulation but pH diminution, whereas the softening profile presented three differential steps named early (no significant softening), intermediate (significant softening), and final (dramatic softening).
26627983	3	40	theme	activities	616:625	arg1	increment					593:601	the significantly increment	575:601	the significantly increment of pectinase activities	575:625	At early step the extensive depolymerization of hydrosoluble pectins and the significantly increment of pectinase activities did not correlate with the slight reduction in firmness.
26627983	3	40	theme	activities	616:625	arg1	depolymerization					530:545	the extensive depolymerization	516:545	the extensive depolymerization of hydrosoluble pectins	516:569	At early step the extensive depolymerization of hydrosoluble pectins and the significantly increment of pectinase activities did not correlate with the slight reduction in firmness.
26627983	1	41	theme	compositional	108:120	arg1	changes					122:128	compositional changes	108:128	compositional changes	108:128	Quality and compositional changes were determined in noni fruit harvested at five ripening stages, from dark-green to thaslucent-grayish.
26627983	6	42	theme	cell	1179:1182	arg1	polymers					1189:1196	cell wall polymers	1179:1196	cell wall polymers	1179:1196	Noni ripening is a process conducted by the coordinated action of pectinases and hemicellulases that promote the differential dissasembly of cell wall polymers.
26627983	3	43	theme	slight	654:659	arg1	reduction					661:669	the slight reduction	650:669	the slight reduction in firmness	650:681	At early step the extensive depolymerization of hydrosoluble pectins and the significantly increment of pectinase activities did not correlate with the slight reduction in firmness.
26627983	5	44	theme	hemicellulosic	1003:1016	arg1	fractions					1018:1026	hemicellulosic fractions	1003:1026	hemicellulosic fractions	1003:1026	The final step was accompanied by the most significant reduction in the yield of alcohol-insoluble solids as well as in the composition of uronic acids and neutral sugars; pectinases increased their activity and depolymerization of hemicellulosic fractions occurred.
26627983	4	45	dep	pectinases	729:738	arg1	activities					759:768	activities	759:768	activities	759:768	The intermediate step showed an increment of pectinases and hemicellulases activities.
26627983	2	46	theme	dramatic	481:488	arg1	early					398:402	early	398:402	early	398:402	Fruit ripening was accompanied by acidity and soluble solids accumulation but pH diminution, whereas the softening profile presented three differential steps named early (no significant softening), intermediate (significant softening), and final (dramatic softening).
26627983	2	46	theme	dramatic	481:488	arg1	softening					490:498	dramatic softening	481:498	dramatic softening	481:498	Fruit ripening was accompanied by acidity and soluble solids accumulation but pH diminution, whereas the softening profile presented three differential steps named early (no significant softening), intermediate (significant softening), and final (dramatic softening).
26627983	2	47	dep	early	398:402	arg1	softening					420:428	no significant softening	405:428	no significant softening	405:428	Fruit ripening was accompanied by acidity and soluble solids accumulation but pH diminution, whereas the softening profile presented three differential steps named early (no significant softening), intermediate (significant softening), and final (dramatic softening).
26627983	6	48	theme	wall	1184:1187	arg1	polymers					1189:1196	cell wall polymers	1179:1196	cell wall polymers	1179:1196	Noni ripening is a process conducted by the coordinated action of pectinases and hemicellulases that promote the differential dissasembly of cell wall polymers.
26627983	3	49	from	reduction	661:669	arg1	firmness					674:681	firmness	674:681	firmness	674:681	At early step the extensive depolymerization of hydrosoluble pectins and the significantly increment of pectinase activities did not correlate with the slight reduction in firmness.
26627983	3	50	theme	pectinase	606:614	arg1	activities					616:625	pectinase activities	606:625	pectinase activities	606:625	At early step the extensive depolymerization of hydrosoluble pectins and the significantly increment of pectinase activities did not correlate with the slight reduction in firmness.
26627983	6	51	theme	hemicellulases	1119:1132	arg1	action					1094:1099	the coordinated action	1078:1099	the coordinated action of pectinases and hemicellulases that promote the differential dissasembly of cell wall polymers	1078:1196	Noni ripening is a process conducted by the coordinated action of pectinases and hemicellulases that promote the differential dissasembly of cell wall polymers.
26189325	1	0	theme	blocks	349:354	arg1	properties					261:270	physiochemical properties	246:270	physiochemical properties	246:270	The U.S. Food and Drug Administration defines criteria for the equivalence of Enoxaparin with Lovenox, comprising the equivalence of physiochemical properties, heparin source material and mode of depolymerization, disaccharide building blocks, fragment mapping and sequence of oligosaccharide species, biological and biochemical assays, and in vivo pharmacodynamic profile.
26189325	1	0	theme	blocks	349:354	arg1	mode					301:304	mode	301:304	mode of depolymerization, disaccharide building blocks, fragment mapping and sequence of oligosaccharide species, biological and biochemical assays, and in vivo pharmacodynamic profile	301:484	The U.S. Food and Drug Administration defines criteria for the equivalence of Enoxaparin with Lovenox, comprising the equivalence of physiochemical properties, heparin source material and mode of depolymerization, disaccharide building blocks, fragment mapping and sequence of oligosaccharide species, biological and biochemical assays, and in vivo pharmacodynamic profile.
26189325	1	0	theme	blocks	349:354	arg1	material					288:295	heparin source material	273:295	heparin source material	273:295	The U.S. Food and Drug Administration defines criteria for the equivalence of Enoxaparin with Lovenox, comprising the equivalence of physiochemical properties, heparin source material and mode of depolymerization, disaccharide building blocks, fragment mapping and sequence of oligosaccharide species, biological and biochemical assays, and in vivo pharmacodynamic profile.
26189325	2	1	theme	C	561:561	arg1	experiments					572:582	both (1)H and (1)H-(13)C HSQC NMR experiments	538:582	both (1)H and (1)H-(13)C HSQC NMR experiments	538:582	Chemometric analysis of the NMR spectra, utilizing both (1)H and (1)H-(13)C HSQC NMR experiments, of Lovenox and Enoxaparin, the latter being the generic version of the former, revealed that Lovenox and the four Enoxaparin compounds produced by Sandoz (Enoxaparin and Fibrinox), Winthrop, and Amphastar exhibit dissimilarities in terms of their composition.
26189325	2	2	from	dissimilarities	798:812	arg1	terms					817:821	terms	817:821	terms of their composition	817:842	Chemometric analysis of the NMR spectra, utilizing both (1)H and (1)H-(13)C HSQC NMR experiments, of Lovenox and Enoxaparin, the latter being the generic version of the former, revealed that Lovenox and the four Enoxaparin compounds produced by Sandoz (Enoxaparin and Fibrinox), Winthrop, and Amphastar exhibit dissimilarities in terms of their composition.
26189325	1	3	theme	physiochemical	246:259	arg1	properties					261:270	physiochemical properties	246:270	physiochemical properties	246:270	The U.S. Food and Drug Administration defines criteria for the equivalence of Enoxaparin with Lovenox, comprising the equivalence of physiochemical properties, heparin source material and mode of depolymerization, disaccharide building blocks, fragment mapping and sequence of oligosaccharide species, biological and biochemical assays, and in vivo pharmacodynamic profile.
26189325	2	4	theme	Enoxaparin	600:609	arg1	version					641:647	the generic version	629:647	the generic version of the former	629:661	Chemometric analysis of the NMR spectra, utilizing both (1)H and (1)H-(13)C HSQC NMR experiments, of Lovenox and Enoxaparin, the latter being the generic version of the former, revealed that Lovenox and the four Enoxaparin compounds produced by Sandoz (Enoxaparin and Fibrinox), Winthrop, and Amphastar exhibit dissimilarities in terms of their composition.
26189325	2	4	theme	Enoxaparin	600:609	arg1	latter					616:621	latter	616:621	latter	616:621	Chemometric analysis of the NMR spectra, utilizing both (1)H and (1)H-(13)C HSQC NMR experiments, of Lovenox and Enoxaparin, the latter being the generic version of the former, revealed that Lovenox and the four Enoxaparin compounds produced by Sandoz (Enoxaparin and Fibrinox), Winthrop, and Amphastar exhibit dissimilarities in terms of their composition.
26189325	1	5	theme	properties	261:270	arg1	equivalence					231:241	the equivalence	227:241	the equivalence of physiochemical properties, heparin source material and mode of depolymerization, disaccharide building blocks, fragment mapping and sequence of oligosaccharide species, biological and biochemical assays, and in vivo pharmacodynamic profile	227:484	The U.S. Food and Drug Administration defines criteria for the equivalence of Enoxaparin with Lovenox, comprising the equivalence of physiochemical properties, heparin source material and mode of depolymerization, disaccharide building blocks, fragment mapping and sequence of oligosaccharide species, biological and biochemical assays, and in vivo pharmacodynamic profile.
26189325	0	6	theme	multivariate	90:101	arg1	analysis					103:110	multivariate analysis	90:110	multivariate analysis	90:110	Differentiation of generic enoxaparins marketed in the United States by employing NMR and multivariate analysis.
26189325	1	7	theme	fragment	357:364	arg1	mapping					366:372	fragment mapping	357:372	fragment mapping	357:372	The U.S. Food and Drug Administration defines criteria for the equivalence of Enoxaparin with Lovenox, comprising the equivalence of physiochemical properties, heparin source material and mode of depolymerization, disaccharide building blocks, fragment mapping and sequence of oligosaccharide species, biological and biochemical assays, and in vivo pharmacodynamic profile.
26189325	1	8	theme	in	454:455	arg1	profile					478:484	in vivo pharmacodynamic profile	454:484	in vivo pharmacodynamic profile	454:484	The U.S. Food and Drug Administration defines criteria for the equivalence of Enoxaparin with Lovenox, comprising the equivalence of physiochemical properties, heparin source material and mode of depolymerization, disaccharide building blocks, fragment mapping and sequence of oligosaccharide species, biological and biochemical assays, and in vivo pharmacodynamic profile.
26189325	1	9	dep	Food	122:125	arg1	The					113:115	The	113:115	The	113:115	The U.S. Food and Drug Administration defines criteria for the equivalence of Enoxaparin with Lovenox, comprising the equivalence of physiochemical properties, heparin source material and mode of depolymerization, disaccharide building blocks, fragment mapping and sequence of oligosaccharide species, biological and biochemical assays, and in vivo pharmacodynamic profile.
26189325	2	10	dep	C	561:561	arg1	1					553:553	1	553:553	1	553:553	Chemometric analysis of the NMR spectra, utilizing both (1)H and (1)H-(13)C HSQC NMR experiments, of Lovenox and Enoxaparin, the latter being the generic version of the former, revealed that Lovenox and the four Enoxaparin compounds produced by Sandoz (Enoxaparin and Fibrinox), Winthrop, and Amphastar exhibit dissimilarities in terms of their composition.
26189325	1	11	theme	mapping	366:372	arg1	properties					261:270	physiochemical properties	246:270	physiochemical properties	246:270	The U.S. Food and Drug Administration defines criteria for the equivalence of Enoxaparin with Lovenox, comprising the equivalence of physiochemical properties, heparin source material and mode of depolymerization, disaccharide building blocks, fragment mapping and sequence of oligosaccharide species, biological and biochemical assays, and in vivo pharmacodynamic profile.
26189325	1	11	theme	mapping	366:372	arg1	mode					301:304	mode	301:304	mode of depolymerization, disaccharide building blocks, fragment mapping and sequence of oligosaccharide species, biological and biochemical assays, and in vivo pharmacodynamic profile	301:484	The U.S. Food and Drug Administration defines criteria for the equivalence of Enoxaparin with Lovenox, comprising the equivalence of physiochemical properties, heparin source material and mode of depolymerization, disaccharide building blocks, fragment mapping and sequence of oligosaccharide species, biological and biochemical assays, and in vivo pharmacodynamic profile.
26189325	1	11	theme	mapping	366:372	arg1	material					288:295	heparin source material	273:295	heparin source material	273:295	The U.S. Food and Drug Administration defines criteria for the equivalence of Enoxaparin with Lovenox, comprising the equivalence of physiochemical properties, heparin source material and mode of depolymerization, disaccharide building blocks, fragment mapping and sequence of oligosaccharide species, biological and biochemical assays, and in vivo pharmacodynamic profile.
26189325	2	12	theme	Lovenox	588:594	arg1	version					641:647	the generic version	629:647	the generic version of the former	629:661	Chemometric analysis of the NMR spectra, utilizing both (1)H and (1)H-(13)C HSQC NMR experiments, of Lovenox and Enoxaparin, the latter being the generic version of the former, revealed that Lovenox and the four Enoxaparin compounds produced by Sandoz (Enoxaparin and Fibrinox), Winthrop, and Amphastar exhibit dissimilarities in terms of their composition.
26189325	2	12	theme	Lovenox	588:594	arg1	latter					616:621	latter	616:621	latter	616:621	Chemometric analysis of the NMR spectra, utilizing both (1)H and (1)H-(13)C HSQC NMR experiments, of Lovenox and Enoxaparin, the latter being the generic version of the former, revealed that Lovenox and the four Enoxaparin compounds produced by Sandoz (Enoxaparin and Fibrinox), Winthrop, and Amphastar exhibit dissimilarities in terms of their composition.
26189325	2	13	theme	composition	832:842	arg1	terms					817:821	terms	817:821	terms of their composition	817:842	Chemometric analysis of the NMR spectra, utilizing both (1)H and (1)H-(13)C HSQC NMR experiments, of Lovenox and Enoxaparin, the latter being the generic version of the former, revealed that Lovenox and the four Enoxaparin compounds produced by Sandoz (Enoxaparin and Fibrinox), Winthrop, and Amphastar exhibit dissimilarities in terms of their composition.
26189325	1	14	theme	heparin	273:279	arg1	material					288:295	heparin source material	273:295	heparin source material	273:295	The U.S. Food and Drug Administration defines criteria for the equivalence of Enoxaparin with Lovenox, comprising the equivalence of physiochemical properties, heparin source material and mode of depolymerization, disaccharide building blocks, fragment mapping and sequence of oligosaccharide species, biological and biochemical assays, and in vivo pharmacodynamic profile.
26189325	1	15	theme	pharmacodynamic	462:476	arg1	profile					478:484	in vivo pharmacodynamic profile	454:484	in vivo pharmacodynamic profile	454:484	The U.S. Food and Drug Administration defines criteria for the equivalence of Enoxaparin with Lovenox, comprising the equivalence of physiochemical properties, heparin source material and mode of depolymerization, disaccharide building blocks, fragment mapping and sequence of oligosaccharide species, biological and biochemical assays, and in vivo pharmacodynamic profile.
26189325	2	16	dep	H	546:546	arg1	1					544:544	1	544:544	1	544:544	Chemometric analysis of the NMR spectra, utilizing both (1)H and (1)H-(13)C HSQC NMR experiments, of Lovenox and Enoxaparin, the latter being the generic version of the former, revealed that Lovenox and the four Enoxaparin compounds produced by Sandoz (Enoxaparin and Fibrinox), Winthrop, and Amphastar exhibit dissimilarities in terms of their composition.
26189325	1	17	theme	sequence	378:385	arg1	properties					261:270	physiochemical properties	246:270	physiochemical properties	246:270	The U.S. Food and Drug Administration defines criteria for the equivalence of Enoxaparin with Lovenox, comprising the equivalence of physiochemical properties, heparin source material and mode of depolymerization, disaccharide building blocks, fragment mapping and sequence of oligosaccharide species, biological and biochemical assays, and in vivo pharmacodynamic profile.
26189325	1	17	theme	sequence	378:385	arg1	mode					301:304	mode	301:304	mode of depolymerization, disaccharide building blocks, fragment mapping and sequence of oligosaccharide species, biological and biochemical assays, and in vivo pharmacodynamic profile	301:484	The U.S. Food and Drug Administration defines criteria for the equivalence of Enoxaparin with Lovenox, comprising the equivalence of physiochemical properties, heparin source material and mode of depolymerization, disaccharide building blocks, fragment mapping and sequence of oligosaccharide species, biological and biochemical assays, and in vivo pharmacodynamic profile.
26189325	1	17	theme	sequence	378:385	arg1	material					288:295	heparin source material	273:295	heparin source material	273:295	The U.S. Food and Drug Administration defines criteria for the equivalence of Enoxaparin with Lovenox, comprising the equivalence of physiochemical properties, heparin source material and mode of depolymerization, disaccharide building blocks, fragment mapping and sequence of oligosaccharide species, biological and biochemical assays, and in vivo pharmacodynamic profile.
26189325	1	18	theme	profile	478:484	arg1	depolymerization					309:324	depolymerization	309:324	depolymerization	309:324	The U.S. Food and Drug Administration defines criteria for the equivalence of Enoxaparin with Lovenox, comprising the equivalence of physiochemical properties, heparin source material and mode of depolymerization, disaccharide building blocks, fragment mapping and sequence of oligosaccharide species, biological and biochemical assays, and in vivo pharmacodynamic profile.
26189325	1	18	theme	profile	478:484	arg1	sequence					378:385	sequence	378:385	sequence	378:385	The U.S. Food and Drug Administration defines criteria for the equivalence of Enoxaparin with Lovenox, comprising the equivalence of physiochemical properties, heparin source material and mode of depolymerization, disaccharide building blocks, fragment mapping and sequence of oligosaccharide species, biological and biochemical assays, and in vivo pharmacodynamic profile.
26189325	1	18	theme	profile	478:484	arg1	blocks					349:354	disaccharide building blocks	327:354	disaccharide building blocks	327:354	The U.S. Food and Drug Administration defines criteria for the equivalence of Enoxaparin with Lovenox, comprising the equivalence of physiochemical properties, heparin source material and mode of depolymerization, disaccharide building blocks, fragment mapping and sequence of oligosaccharide species, biological and biochemical assays, and in vivo pharmacodynamic profile.
26189325	1	18	theme	profile	478:484	arg1	mapping					366:372	fragment mapping	357:372	fragment mapping	357:372	The U.S. Food and Drug Administration defines criteria for the equivalence of Enoxaparin with Lovenox, comprising the equivalence of physiochemical properties, heparin source material and mode of depolymerization, disaccharide building blocks, fragment mapping and sequence of oligosaccharide species, biological and biochemical assays, and in vivo pharmacodynamic profile.
26189325	2	19	theme	NMR	568:570	arg1	experiments					572:582	both (1)H and (1)H-(13)C HSQC NMR experiments	538:582	both (1)H and (1)H-(13)C HSQC NMR experiments	538:582	Chemometric analysis of the NMR spectra, utilizing both (1)H and (1)H-(13)C HSQC NMR experiments, of Lovenox and Enoxaparin, the latter being the generic version of the former, revealed that Lovenox and the four Enoxaparin compounds produced by Sandoz (Enoxaparin and Fibrinox), Winthrop, and Amphastar exhibit dissimilarities in terms of their composition.
26189325	1	20	theme	source	281:286	arg1	material					288:295	heparin source material	273:295	heparin source material	273:295	The U.S. Food and Drug Administration defines criteria for the equivalence of Enoxaparin with Lovenox, comprising the equivalence of physiochemical properties, heparin source material and mode of depolymerization, disaccharide building blocks, fragment mapping and sequence of oligosaccharide species, biological and biochemical assays, and in vivo pharmacodynamic profile.
26189325	2	21	theme	HSQC	563:566	arg1	experiments					572:582	both (1)H and (1)H-(13)C HSQC NMR experiments	538:582	both (1)H and (1)H-(13)C HSQC NMR experiments	538:582	Chemometric analysis of the NMR spectra, utilizing both (1)H and (1)H-(13)C HSQC NMR experiments, of Lovenox and Enoxaparin, the latter being the generic version of the former, revealed that Lovenox and the four Enoxaparin compounds produced by Sandoz (Enoxaparin and Fibrinox), Winthrop, and Amphastar exhibit dissimilarities in terms of their composition.
26189325	4	22	from	products	1051:1058	arg1	material					1025:1032	the branded material	1013:1032	the branded material from the generic products	1013:1058	These studies, in addition to chromatographic analysis, highlighted signatures that differentiated the branded material from the generic products.
26189325	0	23	theme	enoxaparins	27:37	arg1	Differentiation					0:14	Differentiation	0:14	Differentiation of generic enoxaparins	0:37	Differentiation of generic enoxaparins marketed in the United States by employing NMR and multivariate analysis.
26189325	1	24	theme	material	288:295	arg1	equivalence					231:241	the equivalence	227:241	the equivalence of physiochemical properties, heparin source material and mode of depolymerization, disaccharide building blocks, fragment mapping and sequence of oligosaccharide species, biological and biochemical assays, and in vivo pharmacodynamic profile	227:484	The U.S. Food and Drug Administration defines criteria for the equivalence of Enoxaparin with Lovenox, comprising the equivalence of physiochemical properties, heparin source material and mode of depolymerization, disaccharide building blocks, fragment mapping and sequence of oligosaccharide species, biological and biochemical assays, and in vivo pharmacodynamic profile.
26189325	4	25	dep	analysis	960:967	arg1	addition					932:939	addition	932:939	addition	932:939	These studies, in addition to chromatographic analysis, highlighted signatures that differentiated the branded material from the generic products.
26189325	3	26	theme	collected	856:864	arg1	samples					866:872	the collected samples	852:872	the collected samples	852:872	All of the collected samples had expiry dates between 2012 and 2015.
26189325	1	27	theme	Enoxaparin	191:200	arg1	equivalence					176:186	the equivalence	172:186	the equivalence of Enoxaparin with Lovenox	172:213	The U.S. Food and Drug Administration defines criteria for the equivalence of Enoxaparin with Lovenox, comprising the equivalence of physiochemical properties, heparin source material and mode of depolymerization, disaccharide building blocks, fragment mapping and sequence of oligosaccharide species, biological and biochemical assays, and in vivo pharmacodynamic profile.
26189325	0	28	theme	generic	19:25	arg1	enoxaparins					27:37	generic enoxaparins	19:37	generic enoxaparins	19:37	Differentiation of generic enoxaparins marketed in the United States by employing NMR and multivariate analysis.
26189325	2	29	theme	H	546:546	arg1	experiments					572:582	both (1)H and (1)H-(13)C HSQC NMR experiments	538:582	both (1)H and (1)H-(13)C HSQC NMR experiments	538:582	Chemometric analysis of the NMR spectra, utilizing both (1)H and (1)H-(13)C HSQC NMR experiments, of Lovenox and Enoxaparin, the latter being the generic version of the former, revealed that Lovenox and the four Enoxaparin compounds produced by Sandoz (Enoxaparin and Fibrinox), Winthrop, and Amphastar exhibit dissimilarities in terms of their composition.
26189325	1	30	theme	oligosaccharide	390:404	arg1	species					406:412	oligosaccharide species	390:412	oligosaccharide species	390:412	The U.S. Food and Drug Administration defines criteria for the equivalence of Enoxaparin with Lovenox, comprising the equivalence of physiochemical properties, heparin source material and mode of depolymerization, disaccharide building blocks, fragment mapping and sequence of oligosaccharide species, biological and biochemical assays, and in vivo pharmacodynamic profile.
26189325	2	31	theme	Enoxaparin	699:708	arg1	compounds					710:718	the four Enoxaparin compounds	690:718	the four Enoxaparin compounds produced by Sandoz (Enoxaparin and Fibrinox), Winthrop, and Amphastar	690:788	Chemometric analysis of the NMR spectra, utilizing both (1)H and (1)H-(13)C HSQC NMR experiments, of Lovenox and Enoxaparin, the latter being the generic version of the former, revealed that Lovenox and the four Enoxaparin compounds produced by Sandoz (Enoxaparin and Fibrinox), Winthrop, and Amphastar exhibit dissimilarities in terms of their composition.
26189325	1	32	theme	mode	301:304	arg1	equivalence					231:241	the equivalence	227:241	the equivalence of physiochemical properties, heparin source material and mode of depolymerization, disaccharide building blocks, fragment mapping and sequence of oligosaccharide species, biological and biochemical assays, and in vivo pharmacodynamic profile	227:484	The U.S. Food and Drug Administration defines criteria for the equivalence of Enoxaparin with Lovenox, comprising the equivalence of physiochemical properties, heparin source material and mode of depolymerization, disaccharide building blocks, fragment mapping and sequence of oligosaccharide species, biological and biochemical assays, and in vivo pharmacodynamic profile.
26189325	1	33	with	Enoxaparin	191:200	arg1	Lovenox					207:213	Lovenox	207:213	Lovenox	207:213	The U.S. Food and Drug Administration defines criteria for the equivalence of Enoxaparin with Lovenox, comprising the equivalence of physiochemical properties, heparin source material and mode of depolymerization, disaccharide building blocks, fragment mapping and sequence of oligosaccharide species, biological and biochemical assays, and in vivo pharmacodynamic profile.
26189325	1	34	theme	species	406:412	arg1	depolymerization					309:324	depolymerization	309:324	depolymerization	309:324	The U.S. Food and Drug Administration defines criteria for the equivalence of Enoxaparin with Lovenox, comprising the equivalence of physiochemical properties, heparin source material and mode of depolymerization, disaccharide building blocks, fragment mapping and sequence of oligosaccharide species, biological and biochemical assays, and in vivo pharmacodynamic profile.
26189325	1	34	theme	species	406:412	arg1	sequence					378:385	sequence	378:385	sequence	378:385	The U.S. Food and Drug Administration defines criteria for the equivalence of Enoxaparin with Lovenox, comprising the equivalence of physiochemical properties, heparin source material and mode of depolymerization, disaccharide building blocks, fragment mapping and sequence of oligosaccharide species, biological and biochemical assays, and in vivo pharmacodynamic profile.
26189325	1	34	theme	species	406:412	arg1	blocks					349:354	disaccharide building blocks	327:354	disaccharide building blocks	327:354	The U.S. Food and Drug Administration defines criteria for the equivalence of Enoxaparin with Lovenox, comprising the equivalence of physiochemical properties, heparin source material and mode of depolymerization, disaccharide building blocks, fragment mapping and sequence of oligosaccharide species, biological and biochemical assays, and in vivo pharmacodynamic profile.
26189325	1	34	theme	species	406:412	arg1	mapping					366:372	fragment mapping	357:372	fragment mapping	357:372	The U.S. Food and Drug Administration defines criteria for the equivalence of Enoxaparin with Lovenox, comprising the equivalence of physiochemical properties, heparin source material and mode of depolymerization, disaccharide building blocks, fragment mapping and sequence of oligosaccharide species, biological and biochemical assays, and in vivo pharmacodynamic profile.
26189325	2	35	dep	version	641:647	arg1	utilizing					528:536	utilizing	528:536	utilizing both (1)H and (1)H-(13)C HSQC NMR experiments	528:582	Chemometric analysis of the NMR spectra, utilizing both (1)H and (1)H-(13)C HSQC NMR experiments, of Lovenox and Enoxaparin, the latter being the generic version of the former, revealed that Lovenox and the four Enoxaparin compounds produced by Sandoz (Enoxaparin and Fibrinox), Winthrop, and Amphastar exhibit dissimilarities in terms of their composition.
26189325	2	36	theme	Chemometric	487:497	arg1	analysis					499:506	Chemometric analysis	487:506	Chemometric analysis of the NMR spectra, utilizing both (1)H and (1)H-(13)C HSQC NMR experiments, of Lovenox and Enoxaparin, the latter being the generic version of the former,	487:662	Chemometric analysis of the NMR spectra, utilizing both (1)H and (1)H-(13)C HSQC NMR experiments, of Lovenox and Enoxaparin, the latter being the generic version of the former, revealed that Lovenox and the four Enoxaparin compounds produced by Sandoz (Enoxaparin and Fibrinox), Winthrop, and Amphastar exhibit dissimilarities in terms of their composition.
26189325	4	37	theme	chromatographic	944:958	arg1	analysis					960:967	chromatographic analysis	944:967	chromatographic analysis	944:967	These studies, in addition to chromatographic analysis, highlighted signatures that differentiated the branded material from the generic products.
26189325	3	38	theme	expiry	878:883	arg1	dates					885:889	expiry dates	878:889	expiry dates between 2012 and 2015	878:911	All of the collected samples had expiry dates between 2012 and 2015.
26189325	0	39	theme	United	55:60	arg1	States					62:67	the United States	51:67	the United States	51:67	Differentiation of generic enoxaparins marketed in the United States by employing NMR and multivariate analysis.
26189325	1	40	theme	depolymerization	309:324	arg1	properties					261:270	physiochemical properties	246:270	physiochemical properties	246:270	The U.S. Food and Drug Administration defines criteria for the equivalence of Enoxaparin with Lovenox, comprising the equivalence of physiochemical properties, heparin source material and mode of depolymerization, disaccharide building blocks, fragment mapping and sequence of oligosaccharide species, biological and biochemical assays, and in vivo pharmacodynamic profile.
26189325	1	40	theme	depolymerization	309:324	arg1	mode					301:304	mode	301:304	mode of depolymerization, disaccharide building blocks, fragment mapping and sequence of oligosaccharide species, biological and biochemical assays, and in vivo pharmacodynamic profile	301:484	The U.S. Food and Drug Administration defines criteria for the equivalence of Enoxaparin with Lovenox, comprising the equivalence of physiochemical properties, heparin source material and mode of depolymerization, disaccharide building blocks, fragment mapping and sequence of oligosaccharide species, biological and biochemical assays, and in vivo pharmacodynamic profile.
26189325	1	40	theme	depolymerization	309:324	arg1	material					288:295	heparin source material	273:295	heparin source material	273:295	The U.S. Food and Drug Administration defines criteria for the equivalence of Enoxaparin with Lovenox, comprising the equivalence of physiochemical properties, heparin source material and mode of depolymerization, disaccharide building blocks, fragment mapping and sequence of oligosaccharide species, biological and biochemical assays, and in vivo pharmacodynamic profile.
26189325	2	41	theme	H-	555:556	arg1	C					561:561	(1)H-(13)C	552:561	(1)H-(13)C	552:561	Chemometric analysis of the NMR spectra, utilizing both (1)H and (1)H-(13)C HSQC NMR experiments, of Lovenox and Enoxaparin, the latter being the generic version of the former, revealed that Lovenox and the four Enoxaparin compounds produced by Sandoz (Enoxaparin and Fibrinox), Winthrop, and Amphastar exhibit dissimilarities in terms of their composition.
26189325	1	42	theme	biological	415:424	arg1	assays					442:447	biological and biochemical assays	415:447	biological and biochemical assays	415:447	The U.S. Food and Drug Administration defines criteria for the equivalence of Enoxaparin with Lovenox, comprising the equivalence of physiochemical properties, heparin source material and mode of depolymerization, disaccharide building blocks, fragment mapping and sequence of oligosaccharide species, biological and biochemical assays, and in vivo pharmacodynamic profile.
26189325	4	43	theme	generic	1043:1049	arg1	products					1051:1058	the generic products	1039:1058	the generic products	1039:1058	These studies, in addition to chromatographic analysis, highlighted signatures that differentiated the branded material from the generic products.
26189325	2	44	theme	generic	633:639	arg1	version					641:647	the generic version	629:647	the generic version of the former	629:661	Chemometric analysis of the NMR spectra, utilizing both (1)H and (1)H-(13)C HSQC NMR experiments, of Lovenox and Enoxaparin, the latter being the generic version of the former, revealed that Lovenox and the four Enoxaparin compounds produced by Sandoz (Enoxaparin and Fibrinox), Winthrop, and Amphastar exhibit dissimilarities in terms of their composition.
26189325	2	44	theme	generic	633:639	arg1	latter					616:621	latter	616:621	latter	616:621	Chemometric analysis of the NMR spectra, utilizing both (1)H and (1)H-(13)C HSQC NMR experiments, of Lovenox and Enoxaparin, the latter being the generic version of the former, revealed that Lovenox and the four Enoxaparin compounds produced by Sandoz (Enoxaparin and Fibrinox), Winthrop, and Amphastar exhibit dissimilarities in terms of their composition.
26189325	1	45	theme	U.S.	117:120	arg1	Food					122:125	U.S. Food	117:125	U.S. Food	117:125	The U.S. Food and Drug Administration defines criteria for the equivalence of Enoxaparin with Lovenox, comprising the equivalence of physiochemical properties, heparin source material and mode of depolymerization, disaccharide building blocks, fragment mapping and sequence of oligosaccharide species, biological and biochemical assays, and in vivo pharmacodynamic profile.
26189325	1	46	theme	disaccharide	327:338	arg1	blocks					349:354	disaccharide building blocks	327:354	disaccharide building blocks	327:354	The U.S. Food and Drug Administration defines criteria for the equivalence of Enoxaparin with Lovenox, comprising the equivalence of physiochemical properties, heparin source material and mode of depolymerization, disaccharide building blocks, fragment mapping and sequence of oligosaccharide species, biological and biochemical assays, and in vivo pharmacodynamic profile.
26189325	2	47	dep	spectra	519:525	arg1	version					641:647	the generic version	629:647	the generic version of the former	629:661	Chemometric analysis of the NMR spectra, utilizing both (1)H and (1)H-(13)C HSQC NMR experiments, of Lovenox and Enoxaparin, the latter being the generic version of the former, revealed that Lovenox and the four Enoxaparin compounds produced by Sandoz (Enoxaparin and Fibrinox), Winthrop, and Amphastar exhibit dissimilarities in terms of their composition.
26189325	2	47	dep	spectra	519:525	arg1	latter					616:621	latter	616:621	latter	616:621	Chemometric analysis of the NMR spectra, utilizing both (1)H and (1)H-(13)C HSQC NMR experiments, of Lovenox and Enoxaparin, the latter being the generic version of the former, revealed that Lovenox and the four Enoxaparin compounds produced by Sandoz (Enoxaparin and Fibrinox), Winthrop, and Amphastar exhibit dissimilarities in terms of their composition.
26189325	1	48	theme	biochemical	430:440	arg1	assays					442:447	biological and biochemical assays	415:447	biological and biochemical assays	415:447	The U.S. Food and Drug Administration defines criteria for the equivalence of Enoxaparin with Lovenox, comprising the equivalence of physiochemical properties, heparin source material and mode of depolymerization, disaccharide building blocks, fragment mapping and sequence of oligosaccharide species, biological and biochemical assays, and in vivo pharmacodynamic profile.
26189325	1	49	theme	assays	442:447	arg1	depolymerization					309:324	depolymerization	309:324	depolymerization	309:324	The U.S. Food and Drug Administration defines criteria for the equivalence of Enoxaparin with Lovenox, comprising the equivalence of physiochemical properties, heparin source material and mode of depolymerization, disaccharide building blocks, fragment mapping and sequence of oligosaccharide species, biological and biochemical assays, and in vivo pharmacodynamic profile.
26189325	1	49	theme	assays	442:447	arg1	sequence					378:385	sequence	378:385	sequence	378:385	The U.S. Food and Drug Administration defines criteria for the equivalence of Enoxaparin with Lovenox, comprising the equivalence of physiochemical properties, heparin source material and mode of depolymerization, disaccharide building blocks, fragment mapping and sequence of oligosaccharide species, biological and biochemical assays, and in vivo pharmacodynamic profile.
26189325	1	49	theme	assays	442:447	arg1	blocks					349:354	disaccharide building blocks	327:354	disaccharide building blocks	327:354	The U.S. Food and Drug Administration defines criteria for the equivalence of Enoxaparin with Lovenox, comprising the equivalence of physiochemical properties, heparin source material and mode of depolymerization, disaccharide building blocks, fragment mapping and sequence of oligosaccharide species, biological and biochemical assays, and in vivo pharmacodynamic profile.
26189325	1	49	theme	assays	442:447	arg1	mapping					366:372	fragment mapping	357:372	fragment mapping	357:372	The U.S. Food and Drug Administration defines criteria for the equivalence of Enoxaparin with Lovenox, comprising the equivalence of physiochemical properties, heparin source material and mode of depolymerization, disaccharide building blocks, fragment mapping and sequence of oligosaccharide species, biological and biochemical assays, and in vivo pharmacodynamic profile.
26189325	4	50	theme	branded	1017:1023	arg1	material					1025:1032	the branded material	1013:1032	the branded material from the generic products	1013:1058	These studies, in addition to chromatographic analysis, highlighted signatures that differentiated the branded material from the generic products.
26189325	2	51	theme	spectra	519:525	arg1	analysis					499:506	Chemometric analysis	487:506	Chemometric analysis of the NMR spectra, utilizing both (1)H and (1)H-(13)C HSQC NMR experiments, of Lovenox and Enoxaparin, the latter being the generic version of the former,	487:662	Chemometric analysis of the NMR spectra, utilizing both (1)H and (1)H-(13)C HSQC NMR experiments, of Lovenox and Enoxaparin, the latter being the generic version of the former, revealed that Lovenox and the four Enoxaparin compounds produced by Sandoz (Enoxaparin and Fibrinox), Winthrop, and Amphastar exhibit dissimilarities in terms of their composition.
26189325	2	52	dep	Sandoz	732:737	arg1	Fibrinox					755:762	Fibrinox	755:762	Fibrinox	755:762	Chemometric analysis of the NMR spectra, utilizing both (1)H and (1)H-(13)C HSQC NMR experiments, of Lovenox and Enoxaparin, the latter being the generic version of the former, revealed that Lovenox and the four Enoxaparin compounds produced by Sandoz (Enoxaparin and Fibrinox), Winthrop, and Amphastar exhibit dissimilarities in terms of their composition.
26189325	2	52	dep	Sandoz	732:737	arg1	Enoxaparin					740:749	Enoxaparin	740:749	Enoxaparin	740:749	Chemometric analysis of the NMR spectra, utilizing both (1)H and (1)H-(13)C HSQC NMR experiments, of Lovenox and Enoxaparin, the latter being the generic version of the former, revealed that Lovenox and the four Enoxaparin compounds produced by Sandoz (Enoxaparin and Fibrinox), Winthrop, and Amphastar exhibit dissimilarities in terms of their composition.
26189325	1	53	theme	Drug	131:134	arg1	Administration					136:149	Drug Administration	131:149	Drug Administration	131:149	The U.S. Food and Drug Administration defines criteria for the equivalence of Enoxaparin with Lovenox, comprising the equivalence of physiochemical properties, heparin source material and mode of depolymerization, disaccharide building blocks, fragment mapping and sequence of oligosaccharide species, biological and biochemical assays, and in vivo pharmacodynamic profile.
26189325	1	54	dep	in	454:455	arg1	vivo					457:460	vivo	457:460	vivo	457:460	The U.S. Food and Drug Administration defines criteria for the equivalence of Enoxaparin with Lovenox, comprising the equivalence of physiochemical properties, heparin source material and mode of depolymerization, disaccharide building blocks, fragment mapping and sequence of oligosaccharide species, biological and biochemical assays, and in vivo pharmacodynamic profile.
26189325	2	55	theme	NMR	515:517	arg1	spectra					519:525	the NMR spectra	511:525	the NMR spectra	511:525	Chemometric analysis of the NMR spectra, utilizing both (1)H and (1)H-(13)C HSQC NMR experiments, of Lovenox and Enoxaparin, the latter being the generic version of the former, revealed that Lovenox and the four Enoxaparin compounds produced by Sandoz (Enoxaparin and Fibrinox), Winthrop, and Amphastar exhibit dissimilarities in terms of their composition.
26189325	1	56	theme	building	340:347	arg1	blocks					349:354	disaccharide building blocks	327:354	disaccharide building blocks	327:354	The U.S. Food and Drug Administration defines criteria for the equivalence of Enoxaparin with Lovenox, comprising the equivalence of physiochemical properties, heparin source material and mode of depolymerization, disaccharide building blocks, fragment mapping and sequence of oligosaccharide species, biological and biochemical assays, and in vivo pharmacodynamic profile.
26189325	2	57	theme	former	656:661	arg1	version					641:647	the generic version	629:647	the generic version of the former	629:661	Chemometric analysis of the NMR spectra, utilizing both (1)H and (1)H-(13)C HSQC NMR experiments, of Lovenox and Enoxaparin, the latter being the generic version of the former, revealed that Lovenox and the four Enoxaparin compounds produced by Sandoz (Enoxaparin and Fibrinox), Winthrop, and Amphastar exhibit dissimilarities in terms of their composition.
26189325	2	57	theme	former	656:661	arg1	latter					616:621	latter	616:621	latter	616:621	Chemometric analysis of the NMR spectra, utilizing both (1)H and (1)H-(13)C HSQC NMR experiments, of Lovenox and Enoxaparin, the latter being the generic version of the former, revealed that Lovenox and the four Enoxaparin compounds produced by Sandoz (Enoxaparin and Fibrinox), Winthrop, and Amphastar exhibit dissimilarities in terms of their composition.
26189325	3	58	contain	had	874:876	arg1	samples					866:872	the collected samples	852:872	the collected samples	852:872	All of the collected samples had expiry dates between 2012 and 2015.
26189325	3	58	contain	had	874:876	arg2	dates					885:889	expiry dates	878:889	expiry dates between 2012 and 2015	878:911	All of the collected samples had expiry dates between 2012 and 2015.
26189325	3	58	contain	had	874:876	arg1	All					845:847	All	845:847	All	845:847	All of the collected samples had expiry dates between 2012 and 2015.
27693339	8	0	theme	MTT	1014:1016	arg1	assay					1018:1022	MTT assay	1014:1022	MTT assay	1014:1022	Finally, cytotoxicity was studied by MTT assay and the films were non-toxic.
27693339	3	1	theme	films	542:546	arg1	surface					531:537	the surface	527:537	the surface of films	527:546	The morphology studies confirmed that the presence of Mimosa tenuiflora change the surface of films.
27693339	1	2	theme	different	173:181	arg1	ratios					190:195	different weight ratios	173:195	different weight ratios of chitosan	173:207	Bio-composites films were prepared by casting and drying of aqueous solutions containing different weight ratios of chitosan and bark of Mimosa tenuiflora.
27693339	5	3	theme	polymeric	758:766	arg1	composition					768:778	polymeric composition	758:778	polymeric composition of film	758:786	Water-uptake ability changed in relation to polymeric composition of film.
27693339	9	4	theme	chitosan/Mimosa	1111:1125	arg1	films					1138:1142	chitosan/Mimosa tenuiflora films	1111:1142	chitosan/Mimosa tenuiflora films	1111:1142	These preliminary results provide a cheap way to prepare chitosan/Mimosa tenuiflora films for wound healing and skin regeneration.
27693339	4	5	theme	films	636:640	arg1	stability					619:627	the thermal stability	607:627	the thermal stability of the films	607:640	Moreover, the incorporation of Mimosa tenuiflora improved the thermal stability of the films, as it was indicated by the changes in the glass temperatures obtained.
27693339	6	6	theme	tenuiflora	839:848	arg1	addition					820:827	the addition	816:827	the addition of Mimosa tenuiflora to the film	816:860	This property increased by the addition of Mimosa tenuiflora to the film.
27693339	9	7	theme	tenuiflora	1127:1136	arg1	films					1138:1142	chitosan/Mimosa tenuiflora films	1111:1142	chitosan/Mimosa tenuiflora films	1111:1142	These preliminary results provide a cheap way to prepare chitosan/Mimosa tenuiflora films for wound healing and skin regeneration.
27693339	1	8	contain	containing	162:171	arg1	solutions					152:160	aqueous solutions	144:160	aqueous solutions containing different weight ratios of chitosan and bark of Mimosa tenuiflora	144:237	Bio-composites films were prepared by casting and drying of aqueous solutions containing different weight ratios of chitosan and bark of Mimosa tenuiflora.
27693339	1	8	contain	containing	162:171	arg2	bark					213:216	bark	213:216	bark of Mimosa tenuiflora	213:237	Bio-composites films were prepared by casting and drying of aqueous solutions containing different weight ratios of chitosan and bark of Mimosa tenuiflora.
27693339	1	8	contain	containing	162:171	arg2	ratios					190:195	different weight ratios	173:195	different weight ratios of chitosan	173:207	Bio-composites films were prepared by casting and drying of aqueous solutions containing different weight ratios of chitosan and bark of Mimosa tenuiflora.
27693339	6	9	theme	Mimosa	832:837	arg1	tenuiflora					839:848	Mimosa tenuiflora	832:848	Mimosa tenuiflora	832:848	This property increased by the addition of Mimosa tenuiflora to the film.
27693339	2	10	theme	films	294:298	arg1	properties					276:285	The physico-chemical and functional properties	240:285	The physico-chemical and functional properties of the films	240:298	The physico-chemical and functional properties of the films were characterized by scanning electron microscopy, dynamical mechanical analysis, wettability, cytotoxicity and in vitro antibacterial activities.
27693339	1	11	theme	weight	183:188	arg1	ratios					190:195	different weight ratios	173:195	different weight ratios of chitosan	173:207	Bio-composites films were prepared by casting and drying of aqueous solutions containing different weight ratios of chitosan and bark of Mimosa tenuiflora.
27693339	5	12	theme	film	783:786	arg1	composition					768:778	polymeric composition	758:778	polymeric composition of film	758:786	Water-uptake ability changed in relation to polymeric composition of film.
27693339	1	13	theme	Bio-composites	84:97	arg1	films					99:103	Bio-composites films	84:103	Bio-composites films	84:103	Bio-composites films were prepared by casting and drying of aqueous solutions containing different weight ratios of chitosan and bark of Mimosa tenuiflora.
27693339	9	14	theme	wound	1148:1152	arg1	healing					1154:1160	wound healing	1148:1160	wound healing	1148:1160	These preliminary results provide a cheap way to prepare chitosan/Mimosa tenuiflora films for wound healing and skin regeneration.
27693339	2	15	theme	in	413:414	arg1	activities					436:445	in vitro antibacterial activities	413:445	in vitro antibacterial activities	413:445	The physico-chemical and functional properties of the films were characterized by scanning electron microscopy, dynamical mechanical analysis, wettability, cytotoxicity and in vitro antibacterial activities.
27693339	0	16	theme	Chitosan/Mimosa	0:14	arg1	tenuiflora					16:25	Chitosan/Mimosa tenuiflora	0:25	Chitosan/Mimosa tenuiflora	0:25	Chitosan/Mimosa tenuiflora films as potential cellular patch for skin regeneration.
27693339	2	17	theme	electron	331:338	arg1	microscopy					340:349	scanning electron microscopy	322:349	scanning electron microscopy	322:349	The physico-chemical and functional properties of the films were characterized by scanning electron microscopy, dynamical mechanical analysis, wettability, cytotoxicity and in vitro antibacterial activities.
27693339	4	18	from	changes	670:676	arg1	temperatures					691:702	the glass temperatures	681:702	the glass temperatures obtained	681:711	Moreover, the incorporation of Mimosa tenuiflora improved the thermal stability of the films, as it was indicated by the changes in the glass temperatures obtained.
27693339	1	19	theme	chitosan	200:207	arg1	bark					213:216	bark	213:216	bark of Mimosa tenuiflora	213:237	Bio-composites films were prepared by casting and drying of aqueous solutions containing different weight ratios of chitosan and bark of Mimosa tenuiflora.
27693339	1	19	theme	chitosan	200:207	arg1	ratios					190:195	different weight ratios	173:195	different weight ratios of chitosan	173:207	Bio-composites films were prepared by casting and drying of aqueous solutions containing different weight ratios of chitosan and bark of Mimosa tenuiflora.
27693339	4	20	theme	tenuiflora	587:596	arg1	incorporation					563:575	the incorporation	559:575	the incorporation of Mimosa tenuiflora	559:596	Moreover, the incorporation of Mimosa tenuiflora improved the thermal stability of the films, as it was indicated by the changes in the glass temperatures obtained.
27693339	7	21	theme	Improved	863:870	arg1	properties					886:895	Improved antibacterial properties	863:895	Improved antibacterial properties	863:895	Improved antibacterial properties were measured against Escherichia Coli and Micrococcus lysodeikticus or luteus.
27693339	2	22	theme	scanning	322:329	arg1	microscopy					340:349	scanning electron microscopy	322:349	scanning electron microscopy	322:349	The physico-chemical and functional properties of the films were characterized by scanning electron microscopy, dynamical mechanical analysis, wettability, cytotoxicity and in vitro antibacterial activities.
27693339	7	23	theme	antibacterial	872:884	arg1	properties					886:895	Improved antibacterial properties	863:895	Improved antibacterial properties	863:895	Improved antibacterial properties were measured against Escherichia Coli and Micrococcus lysodeikticus or luteus.
27693339	3	24	theme	Mimosa	502:507	arg1	tenuiflora					509:518	Mimosa tenuiflora	502:518	Mimosa tenuiflora	502:518	The morphology studies confirmed that the presence of Mimosa tenuiflora change the surface of films.
27693339	4	25	theme	thermal	611:617	arg1	stability					619:627	the thermal stability	607:627	the thermal stability of the films	607:640	Moreover, the incorporation of Mimosa tenuiflora improved the thermal stability of the films, as it was indicated by the changes in the glass temperatures obtained.
27693339	9	26	theme	skin	1166:1169	arg1	regeneration					1171:1182	skin regeneration	1166:1182	skin regeneration	1166:1182	These preliminary results provide a cheap way to prepare chitosan/Mimosa tenuiflora films for wound healing and skin regeneration.
27693339	3	27	theme	tenuiflora	509:518	arg1	presence					490:497	the presence	486:497	the presence of Mimosa tenuiflora	486:518	The morphology studies confirmed that the presence of Mimosa tenuiflora change the surface of films.
27693339	0	28	theme	cellular	46:53	arg1	patch					55:59	potential cellular patch	36:59	potential cellular patch for skin regeneration	36:81	Chitosan/Mimosa tenuiflora films as potential cellular patch for skin regeneration.
27693339	2	29	theme	physico-chemical	244:259	arg1	properties					276:285	The physico-chemical and functional properties	240:285	The physico-chemical and functional properties of the films	240:298	The physico-chemical and functional properties of the films were characterized by scanning electron microscopy, dynamical mechanical analysis, wettability, cytotoxicity and in vitro antibacterial activities.
27693339	4	30	theme	glass	685:689	arg1	temperatures					691:702	the glass temperatures	681:702	the glass temperatures obtained	681:711	Moreover, the incorporation of Mimosa tenuiflora improved the thermal stability of the films, as it was indicated by the changes in the glass temperatures obtained.
27693339	2	31	theme	antibacterial	422:434	arg1	activities					436:445	in vitro antibacterial activities	413:445	in vitro antibacterial activities	413:445	The physico-chemical and functional properties of the films were characterized by scanning electron microscopy, dynamical mechanical analysis, wettability, cytotoxicity and in vitro antibacterial activities.
27693339	0	32	theme	potential	36:44	arg1	patch					55:59	potential cellular patch	36:59	potential cellular patch for skin regeneration	36:81	Chitosan/Mimosa tenuiflora films as potential cellular patch for skin regeneration.
27693339	1	33	theme	Mimosa	221:226	arg1	tenuiflora					228:237	Mimosa tenuiflora	221:237	Mimosa tenuiflora	221:237	Bio-composites films were prepared by casting and drying of aqueous solutions containing different weight ratios of chitosan and bark of Mimosa tenuiflora.
27693339	9	34	theme	preliminary	1060:1070	arg1	results					1072:1078	These preliminary results	1054:1078	These preliminary results	1054:1078	These preliminary results provide a cheap way to prepare chitosan/Mimosa tenuiflora films for wound healing and skin regeneration.
27693339	4	35	theme	Mimosa	580:585	arg1	tenuiflora					587:596	Mimosa tenuiflora	580:596	Mimosa tenuiflora	580:596	Moreover, the incorporation of Mimosa tenuiflora improved the thermal stability of the films, as it was indicated by the changes in the glass temperatures obtained.
27693339	2	36	theme	mechanical	362:371	arg1	analysis					373:380	dynamical mechanical analysis	352:380	dynamical mechanical analysis	352:380	The physico-chemical and functional properties of the films were characterized by scanning electron microscopy, dynamical mechanical analysis, wettability, cytotoxicity and in vitro antibacterial activities.
27693339	5	37	theme	Water-uptake	714:725	arg1	ability					727:733	Water-uptake ability	714:733	Water-uptake ability	714:733	Water-uptake ability changed in relation to polymeric composition of film.
27693339	2	38	dep	in	413:414	arg1	vitro					416:420	vitro	416:420	vitro	416:420	The physico-chemical and functional properties of the films were characterized by scanning electron microscopy, dynamical mechanical analysis, wettability, cytotoxicity and in vitro antibacterial activities.
27693339	2	39	theme	dynamical	352:360	arg1	analysis					373:380	dynamical mechanical analysis	352:380	dynamical mechanical analysis	352:380	The physico-chemical and functional properties of the films were characterized by scanning electron microscopy, dynamical mechanical analysis, wettability, cytotoxicity and in vitro antibacterial activities.
27693339	1	40	theme	tenuiflora	228:237	arg1	bark					213:216	bark	213:216	bark of Mimosa tenuiflora	213:237	Bio-composites films were prepared by casting and drying of aqueous solutions containing different weight ratios of chitosan and bark of Mimosa tenuiflora.
27693339	1	40	theme	tenuiflora	228:237	arg1	ratios					190:195	different weight ratios	173:195	different weight ratios of chitosan	173:207	Bio-composites films were prepared by casting and drying of aqueous solutions containing different weight ratios of chitosan and bark of Mimosa tenuiflora.
27693339	2	41	theme	functional	265:274	arg1	properties					276:285	The physico-chemical and functional properties	240:285	The physico-chemical and functional properties of the films	240:298	The physico-chemical and functional properties of the films were characterized by scanning electron microscopy, dynamical mechanical analysis, wettability, cytotoxicity and in vitro antibacterial activities.
27693339	1	42	theme	aqueous	144:150	arg1	solutions					152:160	aqueous solutions	144:160	aqueous solutions containing different weight ratios of chitosan and bark of Mimosa tenuiflora	144:237	Bio-composites films were prepared by casting and drying of aqueous solutions containing different weight ratios of chitosan and bark of Mimosa tenuiflora.
27693339	9	43	theme	cheap	1090:1094	arg1	way					1096:1098	a cheap way	1088:1098	a cheap way to prepare chitosan/Mimosa tenuiflora films for wound healing and skin regeneration	1088:1182	These preliminary results provide a cheap way to prepare chitosan/Mimosa tenuiflora films for wound healing and skin regeneration.
27693339	0	44	theme	skin	65:68	arg1	regeneration					70:81	skin regeneration	65:81	skin regeneration	65:81	Chitosan/Mimosa tenuiflora films as potential cellular patch for skin regeneration.
27693339	3	45	theme	morphology	452:461	arg1	studies					463:469	The morphology studies	448:469	The morphology studies	448:469	The morphology studies confirmed that the presence of Mimosa tenuiflora change the surface of films.
27693339	1	46	theme	solutions	152:160	arg1	drying					134:139	drying	134:139	drying	134:139	Bio-composites films were prepared by casting and drying of aqueous solutions containing different weight ratios of chitosan and bark of Mimosa tenuiflora.
27693339	1	46	theme	solutions	152:160	arg1	casting					122:128	casting	122:128	casting	122:128	Bio-composites films were prepared by casting and drying of aqueous solutions containing different weight ratios of chitosan and bark of Mimosa tenuiflora.
28163228	4	0	theme	Ag0	786:788	arg1	stability					842:850	thermal stability	834:850	thermal stability of the wound dressing	834:872	UV/vis spectroscopy, SEM, TEM, DLS, XPS, XRD and TGA were employed to characterize the structure and composition of the nanocomposite, surface morphology of fiber/fabrics, particle size of Ag-NPs, chemical interactions of Ag0 and HA functional groups, crystallinity and thermal stability of the wound dressing, respectively.
28163228	4	0	theme	Ag0	786:788	arg1	interactions					770:781	chemical interactions	761:781	chemical interactions of Ag0 and HA functional groups	761:813	UV/vis spectroscopy, SEM, TEM, DLS, XPS, XRD and TGA were employed to characterize the structure and composition of the nanocomposite, surface morphology of fiber/fabrics, particle size of Ag-NPs, chemical interactions of Ag0 and HA functional groups, crystallinity and thermal stability of the wound dressing, respectively.
28163228	4	0	theme	Ag0	786:788	arg1	crystallinity					816:828	crystallinity	816:828	crystallinity	816:828	UV/vis spectroscopy, SEM, TEM, DLS, XPS, XRD and TGA were employed to characterize the structure and composition of the nanocomposite, surface morphology of fiber/fabrics, particle size of Ag-NPs, chemical interactions of Ag0 and HA functional groups, crystallinity and thermal stability of the wound dressing, respectively.
28163228	4	0	theme	Ag0	786:788	arg1	size					745:748	particle size	736:748	particle size of Ag-NPs	736:758	UV/vis spectroscopy, SEM, TEM, DLS, XPS, XRD and TGA were employed to characterize the structure and composition of the nanocomposite, surface morphology of fiber/fabrics, particle size of Ag-NPs, chemical interactions of Ag0 and HA functional groups, crystallinity and thermal stability of the wound dressing, respectively.
28163228	4	0	theme	Ag0	786:788	arg1	structure					651:659	the structure	647:659	the structure	647:659	UV/vis spectroscopy, SEM, TEM, DLS, XPS, XRD and TGA were employed to characterize the structure and composition of the nanocomposite, surface morphology of fiber/fabrics, particle size of Ag-NPs, chemical interactions of Ag0 and HA functional groups, crystallinity and thermal stability of the wound dressing, respectively.
28163228	4	0	theme	Ag0	786:788	arg1	composition					665:675	composition	665:675	composition	665:675	UV/vis spectroscopy, SEM, TEM, DLS, XPS, XRD and TGA were employed to characterize the structure and composition of the nanocomposite, surface morphology of fiber/fabrics, particle size of Ag-NPs, chemical interactions of Ag0 and HA functional groups, crystallinity and thermal stability of the wound dressing, respectively.
28163228	4	0	theme	Ag0	786:788	arg1	morphology					707:716	surface morphology	699:716	surface morphology of fiber/fabrics	699:733	UV/vis spectroscopy, SEM, TEM, DLS, XPS, XRD and TGA were employed to characterize the structure and composition of the nanocomposite, surface morphology of fiber/fabrics, particle size of Ag-NPs, chemical interactions of Ag0 and HA functional groups, crystallinity and thermal stability of the wound dressing, respectively.
28163228	7	1	theme	bactericidal	1395:1406	arg1	activity					1408:1415	significant bactericidal activity	1383:1415	significant bactericidal activity	1383:1415	The antibacterial performance of medical HA/Ag-NPs fabrics was evaluated against gram negative bacteria E. coli K12, exhibiting significant bactericidal activity.
28163228	4	2	theme	HA	794:795	arg1	stability					842:850	thermal stability	834:850	thermal stability of the wound dressing	834:872	UV/vis spectroscopy, SEM, TEM, DLS, XPS, XRD and TGA were employed to characterize the structure and composition of the nanocomposite, surface morphology of fiber/fabrics, particle size of Ag-NPs, chemical interactions of Ag0 and HA functional groups, crystallinity and thermal stability of the wound dressing, respectively.
28163228	4	2	theme	HA	794:795	arg1	interactions					770:781	chemical interactions	761:781	chemical interactions of Ag0 and HA functional groups	761:813	UV/vis spectroscopy, SEM, TEM, DLS, XPS, XRD and TGA were employed to characterize the structure and composition of the nanocomposite, surface morphology of fiber/fabrics, particle size of Ag-NPs, chemical interactions of Ag0 and HA functional groups, crystallinity and thermal stability of the wound dressing, respectively.
28163228	4	2	theme	HA	794:795	arg1	crystallinity					816:828	crystallinity	816:828	crystallinity	816:828	UV/vis spectroscopy, SEM, TEM, DLS, XPS, XRD and TGA were employed to characterize the structure and composition of the nanocomposite, surface morphology of fiber/fabrics, particle size of Ag-NPs, chemical interactions of Ag0 and HA functional groups, crystallinity and thermal stability of the wound dressing, respectively.
28163228	4	2	theme	HA	794:795	arg1	size					745:748	particle size	736:748	particle size of Ag-NPs	736:758	UV/vis spectroscopy, SEM, TEM, DLS, XPS, XRD and TGA were employed to characterize the structure and composition of the nanocomposite, surface morphology of fiber/fabrics, particle size of Ag-NPs, chemical interactions of Ag0 and HA functional groups, crystallinity and thermal stability of the wound dressing, respectively.
28163228	4	2	theme	HA	794:795	arg1	structure					651:659	the structure	647:659	the structure	647:659	UV/vis spectroscopy, SEM, TEM, DLS, XPS, XRD and TGA were employed to characterize the structure and composition of the nanocomposite, surface morphology of fiber/fabrics, particle size of Ag-NPs, chemical interactions of Ag0 and HA functional groups, crystallinity and thermal stability of the wound dressing, respectively.
28163228	4	2	theme	HA	794:795	arg1	composition					665:675	composition	665:675	composition	665:675	UV/vis spectroscopy, SEM, TEM, DLS, XPS, XRD and TGA were employed to characterize the structure and composition of the nanocomposite, surface morphology of fiber/fabrics, particle size of Ag-NPs, chemical interactions of Ag0 and HA functional groups, crystallinity and thermal stability of the wound dressing, respectively.
28163228	4	2	theme	HA	794:795	arg1	morphology					707:716	surface morphology	699:716	surface morphology of fiber/fabrics	699:733	UV/vis spectroscopy, SEM, TEM, DLS, XPS, XRD and TGA were employed to characterize the structure and composition of the nanocomposite, surface morphology of fiber/fabrics, particle size of Ag-NPs, chemical interactions of Ag0 and HA functional groups, crystallinity and thermal stability of the wound dressing, respectively.
28163228	7	3	theme	bacteria	1350:1357	arg1	K12					1367:1369	gram negative bacteria E. coli K12	1336:1369	gram negative bacteria E. coli K12	1336:1369	The antibacterial performance of medical HA/Ag-NPs fabrics was evaluated against gram negative bacteria E. coli K12, exhibiting significant bactericidal activity.
28163228	10	4	theme	cell	1848:1851	arg1	carrier					1853:1859	cell carrier	1848:1859	wounds and chronic ulcers as well as cell carrier in different biological research and tissue engineering	1811:1915	Based on our results, the new HA/Ag-NPs-2mg nonwoven wound dressing fabrics can be used in treating wounds and chronic ulcers as well as cell carrier in different biological research and tissue engineering.
28163228	2	5	theme	Different	259:267	arg1	concentrations					269:282	Different concentrations	259:282	Different concentrations of silver nitrate (1, 2mg/100ml)	259:315	Different concentrations of silver nitrate (1, 2mg/100ml) were added at ambient temperature to sodium hyaluronate solution (40mg/ml), then the pH was increased to 8 by adding sodium hydroxide.
28163228	1	6	theme	first	247:251	arg1	time					253:256	the first time	243:256	the first time	243:256	In-situ formed hyaluronan/silver (HA/Ag) nanoparticles (NPs) were used to prepare composite fibers/fabrics for the first time.
28163228	7	7	theme	gram	1336:1339	arg1	K12					1367:1369	gram negative bacteria E. coli K12	1336:1369	gram negative bacteria E. coli K12	1336:1369	The antibacterial performance of medical HA/Ag-NPs fabrics was evaluated against gram negative bacteria E. coli K12, exhibiting significant bactericidal activity.
28163228	9	8	theme	non-diabetics/diabetics	1598:1620	arg1	model					1626:1630	non-diabetics/diabetics rat model	1598:1630	non-diabetics/diabetics rat model	1598:1630	In-vivo animal tests indicated that the prepared wound dressing has strong healing efficacy (non-diabetics/diabetics rat model) compared to the plain HA fabrics and greatly accelerated the healing process.
28163228	9	8	theme	non-diabetics/diabetics	1598:1620	arg1	efficacy					1588:1595	strong healing efficacy	1573:1595	strong healing efficacy (non-diabetics/diabetics rat model)	1573:1631	In-vivo animal tests indicated that the prepared wound dressing has strong healing efficacy (non-diabetics/diabetics rat model) compared to the plain HA fabrics and greatly accelerated the healing process.
28163228	9	9	theme	plain	1649:1653	arg1	fabrics					1658:1664	the plain HA fabrics	1645:1664	the plain HA fabrics	1645:1664	In-vivo animal tests indicated that the prepared wound dressing has strong healing efficacy (non-diabetics/diabetics rat model) compared to the plain HA fabrics and greatly accelerated the healing process.
28163228	5	10	dep	showed	939:944	arg1	dispersed					956:964	dispersed	956:964	showed uniformly dispersed throughout HA fiber/fabrics (SEM), an excellent distribution of Ag-NPs with 25±2, nm size (TEM, DLS) and acceptable mechanical properties	939:1102	The resultant HA/Ag-NPs1 and HA/Ag-NPs2 composite showed uniformly dispersed throughout HA fiber/fabrics (SEM), an excellent distribution of Ag-NPs with 25±2, nm size (TEM, DLS) and acceptable mechanical properties.
28163228	10	11	theme	biological	1874:1883	arg1	research					1885:1892	different biological research	1864:1892	different biological research	1864:1892	Based on our results, the new HA/Ag-NPs-2mg nonwoven wound dressing fabrics can be used in treating wounds and chronic ulcers as well as cell carrier in different biological research and tissue engineering.
28163228	6	12	theme	in-situ	1138:1144	arg1	preparation					1146:1156	the in-situ preparation	1134:1156	the in-situ preparation of Ag-NPs	1134:1166	The XRD analysis showed that the in-situ preparation of Ag-NPs increased the crystallinity of the resultant fabrics as well as the thermal stability.
28163228	2	13	theme	nitrate	294:300	arg1	concentrations					269:282	Different concentrations	259:282	Different concentrations of silver nitrate (1, 2mg/100ml)	259:315	Different concentrations of silver nitrate (1, 2mg/100ml) were added at ambient temperature to sodium hyaluronate solution (40mg/ml), then the pH was increased to 8 by adding sodium hydroxide.
28163228	10	14	from	ulcers	1830:1835	arg1	research					1885:1892	different biological research	1864:1892	different biological research	1864:1892	Based on our results, the new HA/Ag-NPs-2mg nonwoven wound dressing fabrics can be used in treating wounds and chronic ulcers as well as cell carrier in different biological research and tissue engineering.
28163228	10	14	from	ulcers	1830:1835	arg1	engineering					1905:1915	tissue engineering	1898:1915	tissue engineering	1898:1915	Based on our results, the new HA/Ag-NPs-2mg nonwoven wound dressing fabrics can be used in treating wounds and chronic ulcers as well as cell carrier in different biological research and tissue engineering.
28163228	4	15	theme	thermal	834:840	arg1	stability					842:850	thermal stability	834:850	thermal stability of the wound dressing	834:872	UV/vis spectroscopy, SEM, TEM, DLS, XPS, XRD and TGA were employed to characterize the structure and composition of the nanocomposite, surface morphology of fiber/fabrics, particle size of Ag-NPs, chemical interactions of Ag0 and HA functional groups, crystallinity and thermal stability of the wound dressing, respectively.
28163228	7	16	theme	fabrics	1306:1312	arg1	performance					1273:1283	The antibacterial performance	1255:1283	The antibacterial performance of medical HA/Ag-NPs fabrics	1255:1312	The antibacterial performance of medical HA/Ag-NPs fabrics was evaluated against gram negative bacteria E. coli K12, exhibiting significant bactericidal activity.
28163228	3	17	theme	fibers/nonwoven	502:516	arg1	fabrics					518:524	fibers/nonwoven fabrics	502:524	fibers/nonwoven fabrics	502:524	The in-situ formed HA/Ag-NPs were used to prepare fibers/nonwoven fabrics by wet-dry-spinning technique (WDST).
28163228	7	18	theme	medical	1288:1294	arg1	fabrics					1306:1312	medical HA/Ag-NPs fabrics	1288:1312	medical HA/Ag-NPs fabrics	1288:1312	The antibacterial performance of medical HA/Ag-NPs fabrics was evaluated against gram negative bacteria E. coli K12, exhibiting significant bactericidal activity.
28163228	10	19	used	used	1794:1797	arg2	fabrics					1779:1785	the new HA/Ag-NPs-2mg nonwoven wound dressing fabrics	1733:1785	the new HA/Ag-NPs-2mg nonwoven wound dressing fabrics	1733:1785	Based on our results, the new HA/Ag-NPs-2mg nonwoven wound dressing fabrics can be used in treating wounds and chronic ulcers as well as cell carrier in different biological research and tissue engineering.
28163228	6	20	theme	fabrics	1213:1219	arg1	crystallinity					1182:1194	the crystallinity	1178:1194	the crystallinity of the resultant fabrics as well as the thermal stability	1178:1252	The XRD analysis showed that the in-situ preparation of Ag-NPs increased the crystallinity of the resultant fabrics as well as the thermal stability.
28163228	5	21	theme	HA	977:978	arg1	SEM					995:997	SEM	995:997	SEM	995:997	The resultant HA/Ag-NPs1 and HA/Ag-NPs2 composite showed uniformly dispersed throughout HA fiber/fabrics (SEM), an excellent distribution of Ag-NPs with 25±2, nm size (TEM, DLS) and acceptable mechanical properties.
28163228	5	21	theme	HA	977:978	arg1	fiber/fabrics					980:992	HA fiber/fabrics	977:992	HA fiber/fabrics (SEM)	977:998	The resultant HA/Ag-NPs1 and HA/Ag-NPs2 composite showed uniformly dispersed throughout HA fiber/fabrics (SEM), an excellent distribution of Ag-NPs with 25±2, nm size (TEM, DLS) and acceptable mechanical properties.
28163228	9	22	theme	strong	1573:1578	arg1	model					1626:1630	non-diabetics/diabetics rat model	1598:1630	non-diabetics/diabetics rat model	1598:1630	In-vivo animal tests indicated that the prepared wound dressing has strong healing efficacy (non-diabetics/diabetics rat model) compared to the plain HA fabrics and greatly accelerated the healing process.
28163228	9	22	theme	strong	1573:1578	arg1	efficacy					1588:1595	strong healing efficacy	1573:1595	strong healing efficacy (non-diabetics/diabetics rat model)	1573:1631	In-vivo animal tests indicated that the prepared wound dressing has strong healing efficacy (non-diabetics/diabetics rat model) compared to the plain HA fabrics and greatly accelerated the healing process.
28163228	9	23	theme	HA	1655:1656	arg1	fabrics					1658:1664	the plain HA fabrics	1645:1664	the plain HA fabrics	1645:1664	In-vivo animal tests indicated that the prepared wound dressing has strong healing efficacy (non-diabetics/diabetics rat model) compared to the plain HA fabrics and greatly accelerated the healing process.
28163228	6	24	theme	XRD	1109:1111	arg1	analysis					1113:1120	The XRD analysis	1105:1120	The XRD analysis	1105:1120	The XRD analysis showed that the in-situ preparation of Ag-NPs increased the crystallinity of the resultant fabrics as well as the thermal stability.
28163228	10	25	theme	HA/Ag-NPs-2mg	1741:1753	arg1	fabrics					1779:1785	the new HA/Ag-NPs-2mg nonwoven wound dressing fabrics	1733:1785	the new HA/Ag-NPs-2mg nonwoven wound dressing fabrics	1733:1785	Based on our results, the new HA/Ag-NPs-2mg nonwoven wound dressing fabrics can be used in treating wounds and chronic ulcers as well as cell carrier in different biological research and tissue engineering.
28163228	7	26	dep	E.	1359:1360	arg1	coli					1362:1365	coli	1362:1365	coli	1362:1365	The antibacterial performance of medical HA/Ag-NPs fabrics was evaluated against gram negative bacteria E. coli K12, exhibiting significant bactericidal activity.
28163228	0	27	theme	chronic	74:80	arg1	dressing					88:95	chronic ulcer dressing	74:95	chronic ulcer dressing	74:95	A novel in situ silver/hyaluronan bio-nanocomposite fabrics for wound and chronic ulcer dressing: In vitro and in vivo evaluations.
28163228	5	28	theme	 nm	1047:1049	arg1	TEM					1057:1059	TEM	1057:1059	TEM	1057:1059	The resultant HA/Ag-NPs1 and HA/Ag-NPs2 composite showed uniformly dispersed throughout HA fiber/fabrics (SEM), an excellent distribution of Ag-NPs with 25±2, nm size (TEM, DLS) and acceptable mechanical properties.
28163228	5	28	theme	 nm	1047:1049	arg1	size					1051:1054	 nm size	1047:1054	 nm size (TEM, DLS)	1047:1065	The resultant HA/Ag-NPs1 and HA/Ag-NPs2 composite showed uniformly dispersed throughout HA fiber/fabrics (SEM), an excellent distribution of Ag-NPs with 25±2, nm size (TEM, DLS) and acceptable mechanical properties.
28163228	10	29	from	carrier	1853:1859	arg1	research					1885:1892	different biological research	1864:1892	different biological research	1864:1892	Based on our results, the new HA/Ag-NPs-2mg nonwoven wound dressing fabrics can be used in treating wounds and chronic ulcers as well as cell carrier in different biological research and tissue engineering.
28163228	10	29	from	carrier	1853:1859	arg1	engineering					1905:1915	tissue engineering	1898:1915	tissue engineering	1898:1915	Based on our results, the new HA/Ag-NPs-2mg nonwoven wound dressing fabrics can be used in treating wounds and chronic ulcers as well as cell carrier in different biological research and tissue engineering.
28163228	1	30	theme	hyaluronan/silver	147:163	arg1	NPs					188:190	NPs	188:190	NPs	188:190	In-situ formed hyaluronan/silver (HA/Ag) nanoparticles (NPs) were used to prepare composite fibers/fabrics for the first time.
28163228	1	30	theme	hyaluronan/silver	147:163	arg1	nanoparticles					173:185	formed hyaluronan/silver (HA/Ag) nanoparticles	140:185	In-situ formed hyaluronan/silver (HA/Ag) nanoparticles (NPs)	132:191	In-situ formed hyaluronan/silver (HA/Ag) nanoparticles (NPs) were used to prepare composite fibers/fabrics for the first time.
28163228	4	31	theme	wound	859:863	arg1	dressing					865:872	the wound dressing	855:872	the wound dressing	855:872	UV/vis spectroscopy, SEM, TEM, DLS, XPS, XRD and TGA were employed to characterize the structure and composition of the nanocomposite, surface morphology of fiber/fabrics, particle size of Ag-NPs, chemical interactions of Ag0 and HA functional groups, crystallinity and thermal stability of the wound dressing, respectively.
28163228	7	32	theme	E.	1359:1360	arg1	K12					1367:1369	gram negative bacteria E. coli K12	1336:1369	gram negative bacteria E. coli K12	1336:1369	The antibacterial performance of medical HA/Ag-NPs fabrics was evaluated against gram negative bacteria E. coli K12, exhibiting significant bactericidal activity.
28163228	0	33	dep	In	98:99	arg1	vitro					101:105	vitro	101:105	vitro	101:105	A novel in situ silver/hyaluronan bio-nanocomposite fabrics for wound and chronic ulcer dressing: In vitro and in vivo evaluations.
28163228	2	34	theme	hyaluronate	361:371	arg1	40mg/ml					383:389	40mg/ml	383:389	40mg/ml	383:389	Different concentrations of silver nitrate (1, 2mg/100ml) were added at ambient temperature to sodium hyaluronate solution (40mg/ml), then the pH was increased to 8 by adding sodium hydroxide.
28163228	2	34	theme	hyaluronate	361:371	arg1	solution					373:380	sodium hyaluronate solution	354:380	sodium hyaluronate solution (40mg/ml)	354:390	Different concentrations of silver nitrate (1, 2mg/100ml) were added at ambient temperature to sodium hyaluronate solution (40mg/ml), then the pH was increased to 8 by adding sodium hydroxide.
28163228	0	35	theme	In	98:99	arg1	evaluations					119:129	In vitro and in vivo evaluations	98:129	A novel in situ silver/hyaluronan bio-nanocomposite fabrics for wound and chronic ulcer dressing: In vitro and in vivo evaluations.	0:130	A novel in situ silver/hyaluronan bio-nanocomposite fabrics for wound and chronic ulcer dressing: In vitro and in vivo evaluations.
28163228	5	36	theme	Ag-NPs	1030:1035	arg1	fiber/fabrics					980:992	HA fiber/fabrics	977:992	HA fiber/fabrics (SEM)	977:998	The resultant HA/Ag-NPs1 and HA/Ag-NPs2 composite showed uniformly dispersed throughout HA fiber/fabrics (SEM), an excellent distribution of Ag-NPs with 25±2, nm size (TEM, DLS) and acceptable mechanical properties.
28163228	5	36	theme	Ag-NPs	1030:1035	arg1	size					1051:1054	 nm size	1047:1054	 nm size (TEM, DLS)	1047:1065	The resultant HA/Ag-NPs1 and HA/Ag-NPs2 composite showed uniformly dispersed throughout HA fiber/fabrics (SEM), an excellent distribution of Ag-NPs with 25±2, nm size (TEM, DLS) and acceptable mechanical properties.
28163228	5	36	theme	Ag-NPs	1030:1035	arg1	distribution					1014:1025	an excellent distribution	1001:1025	an excellent distribution of Ag-NPs with 25±2	1001:1045	The resultant HA/Ag-NPs1 and HA/Ag-NPs2 composite showed uniformly dispersed throughout HA fiber/fabrics (SEM), an excellent distribution of Ag-NPs with 25±2, nm size (TEM, DLS) and acceptable mechanical properties.
28163228	5	36	theme	Ag-NPs	1030:1035	arg1	SEM					995:997	SEM	995:997	SEM	995:997	The resultant HA/Ag-NPs1 and HA/Ag-NPs2 composite showed uniformly dispersed throughout HA fiber/fabrics (SEM), an excellent distribution of Ag-NPs with 25±2, nm size (TEM, DLS) and acceptable mechanical properties.
28163228	5	36	theme	Ag-NPs	1030:1035	arg1	TEM					1057:1059	TEM	1057:1059	TEM	1057:1059	The resultant HA/Ag-NPs1 and HA/Ag-NPs2 composite showed uniformly dispersed throughout HA fiber/fabrics (SEM), an excellent distribution of Ag-NPs with 25±2, nm size (TEM, DLS) and acceptable mechanical properties.
28163228	5	36	theme	Ag-NPs	1030:1035	arg1	properties					1093:1102	acceptable mechanical properties	1071:1102	acceptable mechanical properties	1071:1102	The resultant HA/Ag-NPs1 and HA/Ag-NPs2 composite showed uniformly dispersed throughout HA fiber/fabrics (SEM), an excellent distribution of Ag-NPs with 25±2, nm size (TEM, DLS) and acceptable mechanical properties.
28163228	5	37	theme	resultant	893:901	arg1	HA/Ag-NPs1					903:912	The resultant HA/Ag-NPs1	889:912	The resultant HA/Ag-NPs1	889:912	The resultant HA/Ag-NPs1 and HA/Ag-NPs2 composite showed uniformly dispersed throughout HA fiber/fabrics (SEM), an excellent distribution of Ag-NPs with 25±2, nm size (TEM, DLS) and acceptable mechanical properties.
28163228	5	38	dep	TEM	1057:1059	arg1	DLS					1062:1064	DLS	1062:1064	DLS	1062:1064	The resultant HA/Ag-NPs1 and HA/Ag-NPs2 composite showed uniformly dispersed throughout HA fiber/fabrics (SEM), an excellent distribution of Ag-NPs with 25±2, nm size (TEM, DLS) and acceptable mechanical properties.
28163228	9	39	contain	has	1569:1571	arg2	model					1626:1630	non-diabetics/diabetics rat model	1598:1630	non-diabetics/diabetics rat model	1598:1630	In-vivo animal tests indicated that the prepared wound dressing has strong healing efficacy (non-diabetics/diabetics rat model) compared to the plain HA fabrics and greatly accelerated the healing process.
28163228	9	39	contain	has	1569:1571	arg1	dressing					1560:1567	the prepared wound dressing	1541:1567	the prepared wound dressing	1541:1567	In-vivo animal tests indicated that the prepared wound dressing has strong healing efficacy (non-diabetics/diabetics rat model) compared to the plain HA fabrics and greatly accelerated the healing process.
28163228	9	39	contain	has	1569:1571	arg2	efficacy					1588:1595	strong healing efficacy	1573:1595	strong healing efficacy (non-diabetics/diabetics rat model)	1573:1631	In-vivo animal tests indicated that the prepared wound dressing has strong healing efficacy (non-diabetics/diabetics rat model) compared to the plain HA fabrics and greatly accelerated the healing process.
28163228	8	40	theme	human	1467:1471	arg1	HaCaT					1497:1501	HaCaT	1497:1501	HaCaT	1497:1501	The fibers did not show any cytotoxicity against human keratinocyte cell line (HaCaT).
28163228	8	40	theme	human	1467:1471	arg1	line					1491:1494	human keratinocyte cell line	1467:1494	human keratinocyte cell line (HaCaT)	1467:1502	The fibers did not show any cytotoxicity against human keratinocyte cell line (HaCaT).
28163228	0	41	theme	in	8:9	arg1	fabrics					52:58	A novel in situ silver/hyaluronan bio-nanocomposite fabrics	0:58	A novel in situ silver/hyaluronan bio-nanocomposite fabrics for wound and chronic ulcer dressing: In vitro and in vivo evaluations.	0:130	A novel in situ silver/hyaluronan bio-nanocomposite fabrics for wound and chronic ulcer dressing: In vitro and in vivo evaluations.
28163228	5	42	theme	acceptable	1071:1080	arg1	properties					1093:1102	acceptable mechanical properties	1071:1102	acceptable mechanical properties	1071:1102	The resultant HA/Ag-NPs1 and HA/Ag-NPs2 composite showed uniformly dispersed throughout HA fiber/fabrics (SEM), an excellent distribution of Ag-NPs with 25±2, nm size (TEM, DLS) and acceptable mechanical properties.
28163228	8	43	theme	cell	1486:1489	arg1	HaCaT					1497:1501	HaCaT	1497:1501	HaCaT	1497:1501	The fibers did not show any cytotoxicity against human keratinocyte cell line (HaCaT).
28163228	8	43	theme	cell	1486:1489	arg1	line					1491:1494	human keratinocyte cell line	1467:1494	human keratinocyte cell line (HaCaT)	1467:1502	The fibers did not show any cytotoxicity against human keratinocyte cell line (HaCaT).
28163228	4	44	theme	particle	736:743	arg1	size					745:748	particle size	736:748	particle size of Ag-NPs	736:758	UV/vis spectroscopy, SEM, TEM, DLS, XPS, XRD and TGA were employed to characterize the structure and composition of the nanocomposite, surface morphology of fiber/fabrics, particle size of Ag-NPs, chemical interactions of Ag0 and HA functional groups, crystallinity and thermal stability of the wound dressing, respectively.
28163228	0	45	theme	silver/hyaluronan	16:32	arg1	fabrics					52:58	A novel in situ silver/hyaluronan bio-nanocomposite fabrics	0:58	A novel in situ silver/hyaluronan bio-nanocomposite fabrics for wound and chronic ulcer dressing: In vitro and in vivo evaluations.	0:130	A novel in situ silver/hyaluronan bio-nanocomposite fabrics for wound and chronic ulcer dressing: In vitro and in vivo evaluations.
28163228	9	46	theme	animal	1513:1518	arg1	tests					1520:1524	In-vivo animal tests	1505:1524	In-vivo animal tests	1505:1524	In-vivo animal tests indicated that the prepared wound dressing has strong healing efficacy (non-diabetics/diabetics rat model) compared to the plain HA fabrics and greatly accelerated the healing process.
28163228	10	47	theme	wound	1764:1768	arg1	fabrics					1779:1785	the new HA/Ag-NPs-2mg nonwoven wound dressing fabrics	1733:1785	the new HA/Ag-NPs-2mg nonwoven wound dressing fabrics	1733:1785	Based on our results, the new HA/Ag-NPs-2mg nonwoven wound dressing fabrics can be used in treating wounds and chronic ulcers as well as cell carrier in different biological research and tissue engineering.
28163228	10	48	theme	tissue	1898:1903	arg1	engineering					1905:1915	tissue engineering	1898:1915	tissue engineering	1898:1915	Based on our results, the new HA/Ag-NPs-2mg nonwoven wound dressing fabrics can be used in treating wounds and chronic ulcers as well as cell carrier in different biological research and tissue engineering.
28163228	0	49	dep	fabrics	52:58	arg1	evaluations					119:129	In vitro and in vivo evaluations	98:129	A novel in situ silver/hyaluronan bio-nanocomposite fabrics for wound and chronic ulcer dressing: In vitro and in vivo evaluations.	0:130	A novel in situ silver/hyaluronan bio-nanocomposite fabrics for wound and chronic ulcer dressing: In vitro and in vivo evaluations.
28163228	0	50	dep	in	8:9	arg1	situ					11:14	situ	11:14	situ	11:14	A novel in situ silver/hyaluronan bio-nanocomposite fabrics for wound and chronic ulcer dressing: In vitro and in vivo evaluations.
28163228	4	51	theme	fiber/fabrics	721:733	arg1	stability					842:850	thermal stability	834:850	thermal stability of the wound dressing	834:872	UV/vis spectroscopy, SEM, TEM, DLS, XPS, XRD and TGA were employed to characterize the structure and composition of the nanocomposite, surface morphology of fiber/fabrics, particle size of Ag-NPs, chemical interactions of Ag0 and HA functional groups, crystallinity and thermal stability of the wound dressing, respectively.
28163228	4	51	theme	fiber/fabrics	721:733	arg1	interactions					770:781	chemical interactions	761:781	chemical interactions of Ag0 and HA functional groups	761:813	UV/vis spectroscopy, SEM, TEM, DLS, XPS, XRD and TGA were employed to characterize the structure and composition of the nanocomposite, surface morphology of fiber/fabrics, particle size of Ag-NPs, chemical interactions of Ag0 and HA functional groups, crystallinity and thermal stability of the wound dressing, respectively.
28163228	4	51	theme	fiber/fabrics	721:733	arg1	crystallinity					816:828	crystallinity	816:828	crystallinity	816:828	UV/vis spectroscopy, SEM, TEM, DLS, XPS, XRD and TGA were employed to characterize the structure and composition of the nanocomposite, surface morphology of fiber/fabrics, particle size of Ag-NPs, chemical interactions of Ag0 and HA functional groups, crystallinity and thermal stability of the wound dressing, respectively.
28163228	4	51	theme	fiber/fabrics	721:733	arg1	size					745:748	particle size	736:748	particle size of Ag-NPs	736:758	UV/vis spectroscopy, SEM, TEM, DLS, XPS, XRD and TGA were employed to characterize the structure and composition of the nanocomposite, surface morphology of fiber/fabrics, particle size of Ag-NPs, chemical interactions of Ag0 and HA functional groups, crystallinity and thermal stability of the wound dressing, respectively.
28163228	4	51	theme	fiber/fabrics	721:733	arg1	structure					651:659	the structure	647:659	the structure	647:659	UV/vis spectroscopy, SEM, TEM, DLS, XPS, XRD and TGA were employed to characterize the structure and composition of the nanocomposite, surface morphology of fiber/fabrics, particle size of Ag-NPs, chemical interactions of Ag0 and HA functional groups, crystallinity and thermal stability of the wound dressing, respectively.
28163228	4	51	theme	fiber/fabrics	721:733	arg1	composition					665:675	composition	665:675	composition	665:675	UV/vis spectroscopy, SEM, TEM, DLS, XPS, XRD and TGA were employed to characterize the structure and composition of the nanocomposite, surface morphology of fiber/fabrics, particle size of Ag-NPs, chemical interactions of Ag0 and HA functional groups, crystallinity and thermal stability of the wound dressing, respectively.
28163228	4	51	theme	fiber/fabrics	721:733	arg1	morphology					707:716	surface morphology	699:716	surface morphology of fiber/fabrics	699:733	UV/vis spectroscopy, SEM, TEM, DLS, XPS, XRD and TGA were employed to characterize the structure and composition of the nanocomposite, surface morphology of fiber/fabrics, particle size of Ag-NPs, chemical interactions of Ag0 and HA functional groups, crystallinity and thermal stability of the wound dressing, respectively.
28163228	3	52	theme	in-situ	456:462	arg1	HA/Ag-NPs					471:479	The in-situ formed HA/Ag-NPs	452:479	The in-situ formed HA/Ag-NPs	452:479	The in-situ formed HA/Ag-NPs were used to prepare fibers/nonwoven fabrics by wet-dry-spinning technique (WDST).
28163228	9	53	theme	wound	1554:1558	arg1	dressing					1560:1567	the prepared wound dressing	1541:1567	the prepared wound dressing	1541:1567	In-vivo animal tests indicated that the prepared wound dressing has strong healing efficacy (non-diabetics/diabetics rat model) compared to the plain HA fabrics and greatly accelerated the healing process.
28163228	4	54	theme	UV/vis	564:569	arg1	spectroscopy					571:582	UV/vis spectroscopy	564:582	UV/vis spectroscopy	564:582	UV/vis spectroscopy, SEM, TEM, DLS, XPS, XRD and TGA were employed to characterize the structure and composition of the nanocomposite, surface morphology of fiber/fabrics, particle size of Ag-NPs, chemical interactions of Ag0 and HA functional groups, crystallinity and thermal stability of the wound dressing, respectively.
28163228	10	55	theme	chronic	1822:1828	arg1	ulcers					1830:1835	chronic ulcers	1822:1835	chronic ulcers	1822:1835	Based on our results, the new HA/Ag-NPs-2mg nonwoven wound dressing fabrics can be used in treating wounds and chronic ulcers as well as cell carrier in different biological research and tissue engineering.
28163228	7	56	theme	significant	1383:1393	arg1	activity					1408:1415	significant bactericidal activity	1383:1415	significant bactericidal activity	1383:1415	The antibacterial performance of medical HA/Ag-NPs fabrics was evaluated against gram negative bacteria E. coli K12, exhibiting significant bactericidal activity.
28163228	9	57	theme	healing	1580:1586	arg1	model					1626:1630	non-diabetics/diabetics rat model	1598:1630	non-diabetics/diabetics rat model	1598:1630	In-vivo animal tests indicated that the prepared wound dressing has strong healing efficacy (non-diabetics/diabetics rat model) compared to the plain HA fabrics and greatly accelerated the healing process.
28163228	9	57	theme	healing	1580:1586	arg1	efficacy					1588:1595	strong healing efficacy	1573:1595	strong healing efficacy (non-diabetics/diabetics rat model)	1573:1631	In-vivo animal tests indicated that the prepared wound dressing has strong healing efficacy (non-diabetics/diabetics rat model) compared to the plain HA fabrics and greatly accelerated the healing process.
28163228	1	58	theme	formed	140:145	arg1	NPs					188:190	NPs	188:190	NPs	188:190	In-situ formed hyaluronan/silver (HA/Ag) nanoparticles (NPs) were used to prepare composite fibers/fabrics for the first time.
28163228	1	58	theme	formed	140:145	arg1	nanoparticles					173:185	formed hyaluronan/silver (HA/Ag) nanoparticles	140:185	In-situ formed hyaluronan/silver (HA/Ag) nanoparticles (NPs)	132:191	In-situ formed hyaluronan/silver (HA/Ag) nanoparticles (NPs) were used to prepare composite fibers/fabrics for the first time.
28163228	6	59	theme	Ag-NPs	1161:1166	arg1	preparation					1146:1156	the in-situ preparation	1134:1156	the in-situ preparation of Ag-NPs	1134:1166	The XRD analysis showed that the in-situ preparation of Ag-NPs increased the crystallinity of the resultant fabrics as well as the thermal stability.
28163228	4	60	theme	Ag-NPs	753:758	arg1	stability					842:850	thermal stability	834:850	thermal stability of the wound dressing	834:872	UV/vis spectroscopy, SEM, TEM, DLS, XPS, XRD and TGA were employed to characterize the structure and composition of the nanocomposite, surface morphology of fiber/fabrics, particle size of Ag-NPs, chemical interactions of Ag0 and HA functional groups, crystallinity and thermal stability of the wound dressing, respectively.
28163228	4	60	theme	Ag-NPs	753:758	arg1	interactions					770:781	chemical interactions	761:781	chemical interactions of Ag0 and HA functional groups	761:813	UV/vis spectroscopy, SEM, TEM, DLS, XPS, XRD and TGA were employed to characterize the structure and composition of the nanocomposite, surface morphology of fiber/fabrics, particle size of Ag-NPs, chemical interactions of Ag0 and HA functional groups, crystallinity and thermal stability of the wound dressing, respectively.
28163228	4	60	theme	Ag-NPs	753:758	arg1	crystallinity					816:828	crystallinity	816:828	crystallinity	816:828	UV/vis spectroscopy, SEM, TEM, DLS, XPS, XRD and TGA were employed to characterize the structure and composition of the nanocomposite, surface morphology of fiber/fabrics, particle size of Ag-NPs, chemical interactions of Ag0 and HA functional groups, crystallinity and thermal stability of the wound dressing, respectively.
28163228	4	60	theme	Ag-NPs	753:758	arg1	size					745:748	particle size	736:748	particle size of Ag-NPs	736:758	UV/vis spectroscopy, SEM, TEM, DLS, XPS, XRD and TGA were employed to characterize the structure and composition of the nanocomposite, surface morphology of fiber/fabrics, particle size of Ag-NPs, chemical interactions of Ag0 and HA functional groups, crystallinity and thermal stability of the wound dressing, respectively.
28163228	4	60	theme	Ag-NPs	753:758	arg1	structure					651:659	the structure	647:659	the structure	647:659	UV/vis spectroscopy, SEM, TEM, DLS, XPS, XRD and TGA were employed to characterize the structure and composition of the nanocomposite, surface morphology of fiber/fabrics, particle size of Ag-NPs, chemical interactions of Ag0 and HA functional groups, crystallinity and thermal stability of the wound dressing, respectively.
28163228	4	60	theme	Ag-NPs	753:758	arg1	composition					665:675	composition	665:675	composition	665:675	UV/vis spectroscopy, SEM, TEM, DLS, XPS, XRD and TGA were employed to characterize the structure and composition of the nanocomposite, surface morphology of fiber/fabrics, particle size of Ag-NPs, chemical interactions of Ag0 and HA functional groups, crystallinity and thermal stability of the wound dressing, respectively.
28163228	4	60	theme	Ag-NPs	753:758	arg1	morphology					707:716	surface morphology	699:716	surface morphology of fiber/fabrics	699:733	UV/vis spectroscopy, SEM, TEM, DLS, XPS, XRD and TGA were employed to characterize the structure and composition of the nanocomposite, surface morphology of fiber/fabrics, particle size of Ag-NPs, chemical interactions of Ag0 and HA functional groups, crystallinity and thermal stability of the wound dressing, respectively.
28163228	7	61	theme	negative	1341:1348	arg1	K12					1367:1369	gram negative bacteria E. coli K12	1336:1369	gram negative bacteria E. coli K12	1336:1369	The antibacterial performance of medical HA/Ag-NPs fabrics was evaluated against gram negative bacteria E. coli K12, exhibiting significant bactericidal activity.
28163228	10	62	from	wounds	1811:1816	arg1	research					1885:1892	different biological research	1864:1892	different biological research	1864:1892	Based on our results, the new HA/Ag-NPs-2mg nonwoven wound dressing fabrics can be used in treating wounds and chronic ulcers as well as cell carrier in different biological research and tissue engineering.
28163228	10	62	from	wounds	1811:1816	arg1	engineering					1905:1915	tissue engineering	1898:1915	tissue engineering	1898:1915	Based on our results, the new HA/Ag-NPs-2mg nonwoven wound dressing fabrics can be used in treating wounds and chronic ulcers as well as cell carrier in different biological research and tissue engineering.
28163228	4	63	theme	chemical	761:768	arg1	interactions					770:781	chemical interactions	761:781	chemical interactions of Ag0 and HA functional groups	761:813	UV/vis spectroscopy, SEM, TEM, DLS, XPS, XRD and TGA were employed to characterize the structure and composition of the nanocomposite, surface morphology of fiber/fabrics, particle size of Ag-NPs, chemical interactions of Ag0 and HA functional groups, crystallinity and thermal stability of the wound dressing, respectively.
28163228	3	64	used	used	486:489	arg2	HA/Ag-NPs					471:479	The in-situ formed HA/Ag-NPs	452:479	The in-situ formed HA/Ag-NPs	452:479	The in-situ formed HA/Ag-NPs were used to prepare fibers/nonwoven fabrics by wet-dry-spinning technique (WDST).
28163228	10	65	theme	different	1864:1872	arg1	research					1885:1892	different biological research	1864:1892	different biological research	1864:1892	Based on our results, the new HA/Ag-NPs-2mg nonwoven wound dressing fabrics can be used in treating wounds and chronic ulcers as well as cell carrier in different biological research and tissue engineering.
28163228	6	66	theme	thermal	1236:1242	arg1	stability					1244:1252	the thermal stability	1232:1252	the resultant fabrics as well as the thermal stability	1199:1252	The XRD analysis showed that the in-situ preparation of Ag-NPs increased the crystallinity of the resultant fabrics as well as the thermal stability.
28163228	2	67	theme	silver	287:292	arg1	nitrate					294:300	silver nitrate	287:300	silver nitrate (1, 2mg/100ml)	287:315	Different concentrations of silver nitrate (1, 2mg/100ml) were added at ambient temperature to sodium hyaluronate solution (40mg/ml), then the pH was increased to 8 by adding sodium hydroxide.
28163228	9	68	theme	rat	1622:1624	arg1	model					1626:1630	non-diabetics/diabetics rat model	1598:1630	non-diabetics/diabetics rat model	1598:1630	In-vivo animal tests indicated that the prepared wound dressing has strong healing efficacy (non-diabetics/diabetics rat model) compared to the plain HA fabrics and greatly accelerated the healing process.
28163228	9	68	theme	rat	1622:1624	arg1	efficacy					1588:1595	strong healing efficacy	1573:1595	strong healing efficacy (non-diabetics/diabetics rat model)	1573:1631	In-vivo animal tests indicated that the prepared wound dressing has strong healing efficacy (non-diabetics/diabetics rat model) compared to the plain HA fabrics and greatly accelerated the healing process.
28163228	7	69	theme	HA/Ag-NPs	1296:1304	arg1	fabrics					1306:1312	medical HA/Ag-NPs fabrics	1288:1312	medical HA/Ag-NPs fabrics	1288:1312	The antibacterial performance of medical HA/Ag-NPs fabrics was evaluated against gram negative bacteria E. coli K12, exhibiting significant bactericidal activity.
28163228	9	70	theme	healing	1694:1700	arg1	process					1702:1708	the healing process	1690:1708	the healing process	1690:1708	In-vivo animal tests indicated that the prepared wound dressing has strong healing efficacy (non-diabetics/diabetics rat model) compared to the plain HA fabrics and greatly accelerated the healing process.
28163228	5	71	theme	excellent	1004:1012	arg1	distribution					1014:1025	an excellent distribution	1001:1025	an excellent distribution of Ag-NPs with 25±2	1001:1045	The resultant HA/Ag-NPs1 and HA/Ag-NPs2 composite showed uniformly dispersed throughout HA fiber/fabrics (SEM), an excellent distribution of Ag-NPs with 25±2, nm size (TEM, DLS) and acceptable mechanical properties.
28163228	0	72	theme	in	111:112	arg1	evaluations					119:129	In vitro and in vivo evaluations	98:129	A novel in situ silver/hyaluronan bio-nanocomposite fabrics for wound and chronic ulcer dressing: In vitro and in vivo evaluations.	0:130	A novel in situ silver/hyaluronan bio-nanocomposite fabrics for wound and chronic ulcer dressing: In vitro and in vivo evaluations.
28163228	5	73	with	Ag-NPs	1030:1035	arg1	25±2					1042:1045	25±2	1042:1045	25±2	1042:1045	The resultant HA/Ag-NPs1 and HA/Ag-NPs2 composite showed uniformly dispersed throughout HA fiber/fabrics (SEM), an excellent distribution of Ag-NPs with 25±2, nm size (TEM, DLS) and acceptable mechanical properties.
28163228	6	74	theme	resultant	1203:1211	arg1	fabrics					1213:1219	the resultant fabrics	1199:1219	the resultant fabrics as well as the thermal stability	1199:1252	The XRD analysis showed that the in-situ preparation of Ag-NPs increased the crystallinity of the resultant fabrics as well as the thermal stability.
28163228	10	75	theme	nonwoven	1755:1762	arg1	fabrics					1779:1785	the new HA/Ag-NPs-2mg nonwoven wound dressing fabrics	1733:1785	the new HA/Ag-NPs-2mg nonwoven wound dressing fabrics	1733:1785	Based on our results, the new HA/Ag-NPs-2mg nonwoven wound dressing fabrics can be used in treating wounds and chronic ulcers as well as cell carrier in different biological research and tissue engineering.
28163228	7	76	theme	antibacterial	1259:1271	arg1	performance					1273:1283	The antibacterial performance	1255:1283	The antibacterial performance of medical HA/Ag-NPs fabrics	1255:1312	The antibacterial performance of medical HA/Ag-NPs fabrics was evaluated against gram negative bacteria E. coli K12, exhibiting significant bactericidal activity.
28163228	2	77	dep	nitrate	294:300	arg1	2mg/100ml					306:314	2mg/100ml	306:314	2mg/100ml	306:314	Different concentrations of silver nitrate (1, 2mg/100ml) were added at ambient temperature to sodium hyaluronate solution (40mg/ml), then the pH was increased to 8 by adding sodium hydroxide.
28163228	2	77	dep	nitrate	294:300	arg1	1					303:303	1	303:303	1	303:303	Different concentrations of silver nitrate (1, 2mg/100ml) were added at ambient temperature to sodium hyaluronate solution (40mg/ml), then the pH was increased to 8 by adding sodium hydroxide.
28163228	10	78	theme	new	1737:1739	arg1	fabrics					1779:1785	the new HA/Ag-NPs-2mg nonwoven wound dressing fabrics	1733:1785	the new HA/Ag-NPs-2mg nonwoven wound dressing fabrics	1733:1785	Based on our results, the new HA/Ag-NPs-2mg nonwoven wound dressing fabrics can be used in treating wounds and chronic ulcers as well as cell carrier in different biological research and tissue engineering.
28163228	4	79	theme	functional	797:806	arg1	groups					808:813	functional groups	797:813	functional groups	797:813	UV/vis spectroscopy, SEM, TEM, DLS, XPS, XRD and TGA were employed to characterize the structure and composition of the nanocomposite, surface morphology of fiber/fabrics, particle size of Ag-NPs, chemical interactions of Ag0 and HA functional groups, crystallinity and thermal stability of the wound dressing, respectively.
28163228	2	80	theme	sodium	434:439	arg1	hydroxide					441:449	sodium hydroxide	434:449	sodium hydroxide	434:449	Different concentrations of silver nitrate (1, 2mg/100ml) were added at ambient temperature to sodium hyaluronate solution (40mg/ml), then the pH was increased to 8 by adding sodium hydroxide.
28163228	0	81	theme	ulcer	82:86	arg1	dressing					88:95	chronic ulcer dressing	74:95	chronic ulcer dressing	74:95	A novel in situ silver/hyaluronan bio-nanocomposite fabrics for wound and chronic ulcer dressing: In vitro and in vivo evaluations.
28163228	0	82	dep	in	111:112	arg1	vivo					114:117	vivo	114:117	vivo	114:117	A novel in situ silver/hyaluronan bio-nanocomposite fabrics for wound and chronic ulcer dressing: In vitro and in vivo evaluations.
28163228	4	83	theme	dressing	865:872	arg1	stability					842:850	thermal stability	834:850	thermal stability of the wound dressing	834:872	UV/vis spectroscopy, SEM, TEM, DLS, XPS, XRD and TGA were employed to characterize the structure and composition of the nanocomposite, surface morphology of fiber/fabrics, particle size of Ag-NPs, chemical interactions of Ag0 and HA functional groups, crystallinity and thermal stability of the wound dressing, respectively.
28163228	4	83	theme	dressing	865:872	arg1	interactions					770:781	chemical interactions	761:781	chemical interactions of Ag0 and HA functional groups	761:813	UV/vis spectroscopy, SEM, TEM, DLS, XPS, XRD and TGA were employed to characterize the structure and composition of the nanocomposite, surface morphology of fiber/fabrics, particle size of Ag-NPs, chemical interactions of Ag0 and HA functional groups, crystallinity and thermal stability of the wound dressing, respectively.
28163228	4	83	theme	dressing	865:872	arg1	crystallinity					816:828	crystallinity	816:828	crystallinity	816:828	UV/vis spectroscopy, SEM, TEM, DLS, XPS, XRD and TGA were employed to characterize the structure and composition of the nanocomposite, surface morphology of fiber/fabrics, particle size of Ag-NPs, chemical interactions of Ag0 and HA functional groups, crystallinity and thermal stability of the wound dressing, respectively.
28163228	4	83	theme	dressing	865:872	arg1	size					745:748	particle size	736:748	particle size of Ag-NPs	736:758	UV/vis spectroscopy, SEM, TEM, DLS, XPS, XRD and TGA were employed to characterize the structure and composition of the nanocomposite, surface morphology of fiber/fabrics, particle size of Ag-NPs, chemical interactions of Ag0 and HA functional groups, crystallinity and thermal stability of the wound dressing, respectively.
28163228	4	83	theme	dressing	865:872	arg1	structure					651:659	the structure	647:659	the structure	647:659	UV/vis spectroscopy, SEM, TEM, DLS, XPS, XRD and TGA were employed to characterize the structure and composition of the nanocomposite, surface morphology of fiber/fabrics, particle size of Ag-NPs, chemical interactions of Ag0 and HA functional groups, crystallinity and thermal stability of the wound dressing, respectively.
28163228	4	83	theme	dressing	865:872	arg1	composition					665:675	composition	665:675	composition	665:675	UV/vis spectroscopy, SEM, TEM, DLS, XPS, XRD and TGA were employed to characterize the structure and composition of the nanocomposite, surface morphology of fiber/fabrics, particle size of Ag-NPs, chemical interactions of Ag0 and HA functional groups, crystallinity and thermal stability of the wound dressing, respectively.
28163228	4	83	theme	dressing	865:872	arg1	morphology					707:716	surface morphology	699:716	surface morphology of fiber/fabrics	699:733	UV/vis spectroscopy, SEM, TEM, DLS, XPS, XRD and TGA were employed to characterize the structure and composition of the nanocomposite, surface morphology of fiber/fabrics, particle size of Ag-NPs, chemical interactions of Ag0 and HA functional groups, crystallinity and thermal stability of the wound dressing, respectively.
28163228	4	84	theme	nanocomposite	684:696	arg1	stability					842:850	thermal stability	834:850	thermal stability of the wound dressing	834:872	UV/vis spectroscopy, SEM, TEM, DLS, XPS, XRD and TGA were employed to characterize the structure and composition of the nanocomposite, surface morphology of fiber/fabrics, particle size of Ag-NPs, chemical interactions of Ag0 and HA functional groups, crystallinity and thermal stability of the wound dressing, respectively.
28163228	4	84	theme	nanocomposite	684:696	arg1	interactions					770:781	chemical interactions	761:781	chemical interactions of Ag0 and HA functional groups	761:813	UV/vis spectroscopy, SEM, TEM, DLS, XPS, XRD and TGA were employed to characterize the structure and composition of the nanocomposite, surface morphology of fiber/fabrics, particle size of Ag-NPs, chemical interactions of Ag0 and HA functional groups, crystallinity and thermal stability of the wound dressing, respectively.
28163228	4	84	theme	nanocomposite	684:696	arg1	crystallinity					816:828	crystallinity	816:828	crystallinity	816:828	UV/vis spectroscopy, SEM, TEM, DLS, XPS, XRD and TGA were employed to characterize the structure and composition of the nanocomposite, surface morphology of fiber/fabrics, particle size of Ag-NPs, chemical interactions of Ag0 and HA functional groups, crystallinity and thermal stability of the wound dressing, respectively.
28163228	4	84	theme	nanocomposite	684:696	arg1	size					745:748	particle size	736:748	particle size of Ag-NPs	736:758	UV/vis spectroscopy, SEM, TEM, DLS, XPS, XRD and TGA were employed to characterize the structure and composition of the nanocomposite, surface morphology of fiber/fabrics, particle size of Ag-NPs, chemical interactions of Ag0 and HA functional groups, crystallinity and thermal stability of the wound dressing, respectively.
28163228	4	84	theme	nanocomposite	684:696	arg1	structure					651:659	the structure	647:659	the structure	647:659	UV/vis spectroscopy, SEM, TEM, DLS, XPS, XRD and TGA were employed to characterize the structure and composition of the nanocomposite, surface morphology of fiber/fabrics, particle size of Ag-NPs, chemical interactions of Ag0 and HA functional groups, crystallinity and thermal stability of the wound dressing, respectively.
28163228	4	84	theme	nanocomposite	684:696	arg1	composition					665:675	composition	665:675	composition	665:675	UV/vis spectroscopy, SEM, TEM, DLS, XPS, XRD and TGA were employed to characterize the structure and composition of the nanocomposite, surface morphology of fiber/fabrics, particle size of Ag-NPs, chemical interactions of Ag0 and HA functional groups, crystallinity and thermal stability of the wound dressing, respectively.
28163228	4	84	theme	nanocomposite	684:696	arg1	morphology					707:716	surface morphology	699:716	surface morphology of fiber/fabrics	699:733	UV/vis spectroscopy, SEM, TEM, DLS, XPS, XRD and TGA were employed to characterize the structure and composition of the nanocomposite, surface morphology of fiber/fabrics, particle size of Ag-NPs, chemical interactions of Ag0 and HA functional groups, crystallinity and thermal stability of the wound dressing, respectively.
28163228	2	85	theme	sodium	354:359	arg1	40mg/ml					383:389	40mg/ml	383:389	40mg/ml	383:389	Different concentrations of silver nitrate (1, 2mg/100ml) were added at ambient temperature to sodium hyaluronate solution (40mg/ml), then the pH was increased to 8 by adding sodium hydroxide.
28163228	2	85	theme	sodium	354:359	arg1	solution					373:380	sodium hyaluronate solution	354:380	sodium hyaluronate solution (40mg/ml)	354:390	Different concentrations of silver nitrate (1, 2mg/100ml) were added at ambient temperature to sodium hyaluronate solution (40mg/ml), then the pH was increased to 8 by adding sodium hydroxide.
28163228	0	86	theme	novel	2:6	arg1	fabrics					52:58	A novel in situ silver/hyaluronan bio-nanocomposite fabrics	0:58	A novel in situ silver/hyaluronan bio-nanocomposite fabrics for wound and chronic ulcer dressing: In vitro and in vivo evaluations.	0:130	A novel in situ silver/hyaluronan bio-nanocomposite fabrics for wound and chronic ulcer dressing: In vitro and in vivo evaluations.
28163228	8	87	theme	keratinocyte	1473:1484	arg1	HaCaT					1497:1501	HaCaT	1497:1501	HaCaT	1497:1501	The fibers did not show any cytotoxicity against human keratinocyte cell line (HaCaT).
28163228	8	87	theme	keratinocyte	1473:1484	arg1	line					1491:1494	human keratinocyte cell line	1467:1494	human keratinocyte cell line (HaCaT)	1467:1502	The fibers did not show any cytotoxicity against human keratinocyte cell line (HaCaT).
28163228	9	88	theme	prepared	1545:1552	arg1	dressing					1560:1567	the prepared wound dressing	1541:1567	the prepared wound dressing	1541:1567	In-vivo animal tests indicated that the prepared wound dressing has strong healing efficacy (non-diabetics/diabetics rat model) compared to the plain HA fabrics and greatly accelerated the healing process.
28163228	2	89	theme	ambient	331:337	arg1	temperature					339:349	ambient temperature	331:349	ambient temperature	331:349	Different concentrations of silver nitrate (1, 2mg/100ml) were added at ambient temperature to sodium hyaluronate solution (40mg/ml), then the pH was increased to 8 by adding sodium hydroxide.
28163228	0	90	theme	bio-nanocomposite	34:50	arg1	fabrics					52:58	A novel in situ silver/hyaluronan bio-nanocomposite fabrics	0:58	A novel in situ silver/hyaluronan bio-nanocomposite fabrics for wound and chronic ulcer dressing: In vitro and in vivo evaluations.	0:130	A novel in situ silver/hyaluronan bio-nanocomposite fabrics for wound and chronic ulcer dressing: In vitro and in vivo evaluations.
28163228	5	91	theme	mechanical	1082:1091	arg1	properties					1093:1102	acceptable mechanical properties	1071:1102	acceptable mechanical properties	1071:1102	The resultant HA/Ag-NPs1 and HA/Ag-NPs2 composite showed uniformly dispersed throughout HA fiber/fabrics (SEM), an excellent distribution of Ag-NPs with 25±2, nm size (TEM, DLS) and acceptable mechanical properties.
28163228	9	92	theme	In-vivo	1505:1511	arg1	tests					1520:1524	In-vivo animal tests	1505:1524	In-vivo animal tests	1505:1524	In-vivo animal tests indicated that the prepared wound dressing has strong healing efficacy (non-diabetics/diabetics rat model) compared to the plain HA fabrics and greatly accelerated the healing process.
28163228	10	93	theme	dressing	1770:1777	arg1	fabrics					1779:1785	the new HA/Ag-NPs-2mg nonwoven wound dressing fabrics	1733:1785	the new HA/Ag-NPs-2mg nonwoven wound dressing fabrics	1733:1785	Based on our results, the new HA/Ag-NPs-2mg nonwoven wound dressing fabrics can be used in treating wounds and chronic ulcers as well as cell carrier in different biological research and tissue engineering.
28163228	1	94	dep	nanoparticles	173:185	arg1	In-situ					132:138	In-situ formed hyaluronan/silver (HA/Ag) nanoparticles (NPs)	132:191	In-situ formed hyaluronan/silver (HA/Ag) nanoparticles (NPs)	132:191	In-situ formed hyaluronan/silver (HA/Ag) nanoparticles (NPs) were used to prepare composite fibers/fabrics for the first time.
28163228	6	95	theme	stability	1244:1252	arg1	crystallinity					1182:1194	the crystallinity	1178:1194	the crystallinity of the resultant fabrics as well as the thermal stability	1178:1252	The XRD analysis showed that the in-situ preparation of Ag-NPs increased the crystallinity of the resultant fabrics as well as the thermal stability.
28163228	4	96	dep	Ag0	786:788	arg1	groups					808:813	functional groups	797:813	functional groups	797:813	UV/vis spectroscopy, SEM, TEM, DLS, XPS, XRD and TGA were employed to characterize the structure and composition of the nanocomposite, surface morphology of fiber/fabrics, particle size of Ag-NPs, chemical interactions of Ag0 and HA functional groups, crystallinity and thermal stability of the wound dressing, respectively.
28163228	4	97	theme	surface	699:705	arg1	morphology					707:716	surface morphology	699:716	surface morphology of fiber/fabrics	699:733	UV/vis spectroscopy, SEM, TEM, DLS, XPS, XRD and TGA were employed to characterize the structure and composition of the nanocomposite, surface morphology of fiber/fabrics, particle size of Ag-NPs, chemical interactions of Ag0 and HA functional groups, crystallinity and thermal stability of the wound dressing, respectively.
28163228	1	98	used	used	198:201	arg2	NPs					188:190	NPs	188:190	NPs	188:190	In-situ formed hyaluronan/silver (HA/Ag) nanoparticles (NPs) were used to prepare composite fibers/fabrics for the first time.
28163228	1	98	used	used	198:201	arg2	nanoparticles					173:185	formed hyaluronan/silver (HA/Ag) nanoparticles	140:185	In-situ formed hyaluronan/silver (HA/Ag) nanoparticles (NPs)	132:191	In-situ formed hyaluronan/silver (HA/Ag) nanoparticles (NPs) were used to prepare composite fibers/fabrics for the first time.
28163228	5	99	theme	HA/Ag-NPs2	918:927	arg1	composite					929:937	HA/Ag-NPs2 composite	918:937	HA/Ag-NPs2 composite	918:937	The resultant HA/Ag-NPs1 and HA/Ag-NPs2 composite showed uniformly dispersed throughout HA fiber/fabrics (SEM), an excellent distribution of Ag-NPs with 25±2, nm size (TEM, DLS) and acceptable mechanical properties.
28163228	3	100	theme	formed	464:469	arg1	HA/Ag-NPs					471:479	The in-situ formed HA/Ag-NPs	452:479	The in-situ formed HA/Ag-NPs	452:479	The in-situ formed HA/Ag-NPs were used to prepare fibers/nonwoven fabrics by wet-dry-spinning technique (WDST).
28163228	1	101	theme	composite	214:222	arg1	fibers/fabrics					224:237	composite fibers/fabrics	214:237	composite fibers/fabrics	214:237	In-situ formed hyaluronan/silver (HA/Ag) nanoparticles (NPs) were used to prepare composite fibers/fabrics for the first time.
26256336	7	0	theme	pharmaceutical	1187:1200	arg1	industry					1210:1217	pharmaceutical capsule industry	1187:1217	pharmaceutical capsule industry	1187:1217	These results illustrate that by manipulation of the constituents of sago starch-based composite system, a suitable alternative to gelatin can be produced with comparable properties and this could find potential application in pharmaceutical capsule industry.
26256336	1	1	theme	starch-based	153:164	arg1	system					166:171	Composite sago starch-based system	138:171	Composite sago starch-based system	138:171	Composite sago starch-based system was developed and characterized with the aim to find an alternative to gelatin in the processing of pharmaceutical capsules.
26256336	0	2	theme	starch	75:80	arg1	properties					40:49	rheological properties	28:49	rheological properties of dually modified sago starch: Towards finding gelatin alternative for hard capsules	28:135	Effects of κ-carrageenan on rheological properties of dually modified sago starch: Towards finding gelatin alternative for hard capsules.
26256336	6	3	theme	G	898:898	arg1	moduli					883:888	The viscoelastic moduli	866:888	The viscoelastic moduli (G' and G") for the proposed system	866:924	The viscoelastic moduli (G' and G") for the proposed system were lower than those of gelatin.
26256336	6	3	theme	G	898:898	arg1	"					899:899	G' and G"	891:899	G' and G"	891:899	The viscoelastic moduli (G' and G") for the proposed system were lower than those of gelatin.
26256336	4	4	theme	rheological	682:692	arg1	behavior					694:701	gelatin rheological behavior	674:701	gelatin rheological behavior in pharmaceutical capsule processing	674:738	Results show that combination of HHSS12 (Hydrolysed-hydroxypropylated sago starch at 12h) with 0.5% κ-carrageenan was comparable to gelatin rheological behavior in pharmaceutical capsule processing.
26256336	0	5	from	Effects	0:6	arg1	properties					40:49	rheological properties	28:49	rheological properties of dually modified sago starch: Towards finding gelatin alternative for hard capsules	28:135	Effects of κ-carrageenan on rheological properties of dually modified sago starch: Towards finding gelatin alternative for hard capsules.
26256336	3	6	theme	reference	522:530	arg1	properties					435:444	The rheological properties	419:444	The rheological properties of the proposed composite system	419:477	The rheological properties of the proposed composite system were measured and compared with gelatin as reference material.
26256336	3	6	theme	reference	522:530	arg1	material					532:539	reference material	522:539	reference material	522:539	The rheological properties of the proposed composite system were measured and compared with gelatin as reference material.
26256336	4	7	theme	capsule	721:727	arg1	processing					729:738	pharmaceutical capsule processing	706:738	pharmaceutical capsule processing	706:738	Results show that combination of HHSS12 (Hydrolysed-hydroxypropylated sago starch at 12h) with 0.5% κ-carrageenan was comparable to gelatin rheological behavior in pharmaceutical capsule processing.
26256336	5	8	theme	solution	745:752	arg1	comparable					834:843	comparable	834:843	comparable	834:843	The solution viscosity at 50 °C and sol-gel transition of the proposed composite system were comparable to those of gelatin.
26256336	5	8	theme	solution	745:752	arg1	viscosity					754:762	The solution viscosity	741:762	The solution viscosity at 50 °C and sol-gel transition of the proposed composite system	741:827	The solution viscosity at 50 °C and sol-gel transition of the proposed composite system were comparable to those of gelatin.
26256336	4	9	theme	pharmaceutical	706:719	arg1	processing					729:738	pharmaceutical capsule processing	706:738	pharmaceutical capsule processing	706:738	Results show that combination of HHSS12 (Hydrolysed-hydroxypropylated sago starch at 12h) with 0.5% κ-carrageenan was comparable to gelatin rheological behavior in pharmaceutical capsule processing.
26256336	0	10	theme	gelatin	99:105	arg1	alternative					107:117	gelatin alternative	99:117	gelatin alternative for hard capsules	99:135	Effects of κ-carrageenan on rheological properties of dually modified sago starch: Towards finding gelatin alternative for hard capsules.
26256336	7	11	theme	suitable	1067:1074	arg1	alternative					1076:1086	a suitable alternative	1065:1086	a suitable alternative to gelatin	1065:1097	These results illustrate that by manipulation of the constituents of sago starch-based composite system, a suitable alternative to gelatin can be produced with comparable properties and this could find potential application in pharmaceutical capsule industry.
26256336	4	12	theme	Hydrolysed-hydroxypropylated	583:610	arg1	starch					617:622	Hydrolysed-hydroxypropylated sago starch	583:622	Hydrolysed-hydroxypropylated sago starch at 12h	583:629	Results show that combination of HHSS12 (Hydrolysed-hydroxypropylated sago starch at 12h) with 0.5% κ-carrageenan was comparable to gelatin rheological behavior in pharmaceutical capsule processing.
26256336	4	13	theme	%	640:640	arg1	κ-carrageenan					642:654	0.5% κ-carrageenan	637:654	0.5% κ-carrageenan	637:654	Results show that combination of HHSS12 (Hydrolysed-hydroxypropylated sago starch at 12h) with 0.5% κ-carrageenan was comparable to gelatin rheological behavior in pharmaceutical capsule processing.
26256336	5	14	theme	proposed	803:810	arg1	system					822:827	the proposed composite system	799:827	the proposed composite system	799:827	The solution viscosity at 50 °C and sol-gel transition of the proposed composite system were comparable to those of gelatin.
26256336	7	15	theme	capsule	1202:1208	arg1	industry					1210:1217	pharmaceutical capsule industry	1187:1217	pharmaceutical capsule industry	1187:1217	These results illustrate that by manipulation of the constituents of sago starch-based composite system, a suitable alternative to gelatin can be produced with comparable properties and this could find potential application in pharmaceutical capsule industry.
26256336	7	16	theme	system	1057:1062	arg1	constituents					1013:1024	the constituents	1009:1024	the constituents of sago starch-based composite system	1009:1062	These results illustrate that by manipulation of the constituents of sago starch-based composite system, a suitable alternative to gelatin can be produced with comparable properties and this could find potential application in pharmaceutical capsule industry.
26256336	2	17	theme	Hydrolyzed-Hydroxypropylated	315:342	arg1	starches					350:357	Dually modified (Hydrolyzed-Hydroxypropylated) sago starches	298:357	Dually modified (Hydrolyzed-Hydroxypropylated) sago starches	298:357	Dually modified (Hydrolyzed-Hydroxypropylated) sago starches were combined with κ-carrageenan (0.25, 0.5, 0.75, and 1%).
26256336	6	18	theme	G	891:891	arg1	moduli					883:888	The viscoelastic moduli	866:888	The viscoelastic moduli (G' and G") for the proposed system	866:924	The viscoelastic moduli (G' and G") for the proposed system were lower than those of gelatin.
26256336	6	18	theme	G	891:891	arg1	"					899:899	G' and G"	891:899	G' and G"	891:899	The viscoelastic moduli (G' and G") for the proposed system were lower than those of gelatin.
26256336	5	19	theme	composite	812:820	arg1	system					822:827	the proposed composite system	799:827	the proposed composite system	799:827	The solution viscosity at 50 °C and sol-gel transition of the proposed composite system were comparable to those of gelatin.
26256336	7	20	theme	comparable	1120:1129	arg1	properties					1131:1140	comparable properties	1120:1140	comparable properties	1120:1140	These results illustrate that by manipulation of the constituents of sago starch-based composite system, a suitable alternative to gelatin can be produced with comparable properties and this could find potential application in pharmaceutical capsule industry.
26256336	7	21	theme	starch-based	1034:1045	arg1	system					1057:1062	sago starch-based composite system	1029:1062	sago starch-based composite system	1029:1062	These results illustrate that by manipulation of the constituents of sago starch-based composite system, a suitable alternative to gelatin can be produced with comparable properties and this could find potential application in pharmaceutical capsule industry.
26256336	4	22	dep	HHSS12	575:580	arg1	starch					617:622	Hydrolysed-hydroxypropylated sago starch	583:622	Hydrolysed-hydroxypropylated sago starch at 12h	583:629	Results show that combination of HHSS12 (Hydrolysed-hydroxypropylated sago starch at 12h) with 0.5% κ-carrageenan was comparable to gelatin rheological behavior in pharmaceutical capsule processing.
26256336	5	23	theme	system	822:827	arg1	°C					770:771	50 °C	767:771	50 °C	767:771	The solution viscosity at 50 °C and sol-gel transition of the proposed composite system were comparable to those of gelatin.
26256336	5	23	theme	system	822:827	arg1	transition					785:794	sol-gel transition	777:794	sol-gel transition	777:794	The solution viscosity at 50 °C and sol-gel transition of the proposed composite system were comparable to those of gelatin.
26256336	1	24	theme	pharmaceutical	273:286	arg1	capsules					288:295	pharmaceutical capsules	273:295	pharmaceutical capsules	273:295	Composite sago starch-based system was developed and characterized with the aim to find an alternative to gelatin in the processing of pharmaceutical capsules.
26256336	0	25	theme	hard	123:126	arg1	capsules					128:135	hard capsules	123:135	hard capsules	123:135	Effects of κ-carrageenan on rheological properties of dually modified sago starch: Towards finding gelatin alternative for hard capsules.
26256336	2	26	theme	modified	305:312	arg1	starches					350:357	Dually modified (Hydrolyzed-Hydroxypropylated) sago starches	298:357	Dually modified (Hydrolyzed-Hydroxypropylated) sago starches	298:357	Dually modified (Hydrolyzed-Hydroxypropylated) sago starches were combined with κ-carrageenan (0.25, 0.5, 0.75, and 1%).
26256336	0	27	theme	κ-carrageenan	11:23	arg1	Effects					0:6	Effects	0:6	Effects of κ-carrageenan on rheological properties of dually modified sago starch: Towards finding gelatin alternative for hard capsules.	0:136	Effects of κ-carrageenan on rheological properties of dually modified sago starch: Towards finding gelatin alternative for hard capsules.
26256336	1	28	theme	capsules	288:295	arg1	processing					259:268	the processing	255:268	the processing of pharmaceutical capsules	255:295	Composite sago starch-based system was developed and characterized with the aim to find an alternative to gelatin in the processing of pharmaceutical capsules.
26256336	7	29	theme	composite	1047:1055	arg1	system					1057:1062	sago starch-based composite system	1029:1062	sago starch-based composite system	1029:1062	These results illustrate that by manipulation of the constituents of sago starch-based composite system, a suitable alternative to gelatin can be produced with comparable properties and this could find potential application in pharmaceutical capsule industry.
26256336	6	30	theme	viscoelastic	870:881	arg1	lower					931:935	lower	931:935	lower	931:935	The viscoelastic moduli (G' and G") for the proposed system were lower than those of gelatin.
26256336	6	30	theme	viscoelastic	870:881	arg1	moduli					883:888	The viscoelastic moduli	866:888	The viscoelastic moduli (G' and G") for the proposed system	866:924	The viscoelastic moduli (G' and G") for the proposed system were lower than those of gelatin.
26256336	6	30	theme	viscoelastic	870:881	arg1	"					899:899	G' and G"	891:899	G' and G"	891:899	The viscoelastic moduli (G' and G") for the proposed system were lower than those of gelatin.
26256336	1	31	theme	Composite	138:146	arg1	system					166:171	Composite sago starch-based system	138:171	Composite sago starch-based system	138:171	Composite sago starch-based system was developed and characterized with the aim to find an alternative to gelatin in the processing of pharmaceutical capsules.
26256336	5	32	theme	sol-gel	777:783	arg1	transition					785:794	sol-gel transition	777:794	sol-gel transition	777:794	The solution viscosity at 50 °C and sol-gel transition of the proposed composite system were comparable to those of gelatin.
26256336	4	33	theme	0.5	637:639	arg1	%					640:640	%	640:640	%	640:640	Results show that combination of HHSS12 (Hydrolysed-hydroxypropylated sago starch at 12h) with 0.5% κ-carrageenan was comparable to gelatin rheological behavior in pharmaceutical capsule processing.
26256336	4	34	from	12h	627:629	arg1	starch					617:622	Hydrolysed-hydroxypropylated sago starch	583:622	Hydrolysed-hydroxypropylated sago starch at 12h	583:629	Results show that combination of HHSS12 (Hydrolysed-hydroxypropylated sago starch at 12h) with 0.5% κ-carrageenan was comparable to gelatin rheological behavior in pharmaceutical capsule processing.
26256336	3	35	theme	rheological	423:433	arg1	properties					435:444	The rheological properties	419:444	The rheological properties of the proposed composite system	419:477	The rheological properties of the proposed composite system were measured and compared with gelatin as reference material.
26256336	3	35	theme	rheological	423:433	arg1	material					532:539	reference material	522:539	reference material	522:539	The rheological properties of the proposed composite system were measured and compared with gelatin as reference material.
26256336	0	36	theme	rheological	28:38	arg1	properties					40:49	rheological properties	28:49	rheological properties of dually modified sago starch: Towards finding gelatin alternative for hard capsules	28:135	Effects of κ-carrageenan on rheological properties of dually modified sago starch: Towards finding gelatin alternative for hard capsules.
26256336	7	37	theme	sago	1029:1032	arg1	system					1057:1062	sago starch-based composite system	1029:1062	sago starch-based composite system	1029:1062	These results illustrate that by manipulation of the constituents of sago starch-based composite system, a suitable alternative to gelatin can be produced with comparable properties and this could find potential application in pharmaceutical capsule industry.
26256336	7	38	from	application	1172:1182	arg1	industry					1210:1217	pharmaceutical capsule industry	1187:1217	pharmaceutical capsule industry	1187:1217	These results illustrate that by manipulation of the constituents of sago starch-based composite system, a suitable alternative to gelatin can be produced with comparable properties and this could find potential application in pharmaceutical capsule industry.
26256336	7	39	theme	potential	1162:1170	arg1	application					1172:1182	potential application	1162:1182	potential application in pharmaceutical capsule industry	1162:1217	These results illustrate that by manipulation of the constituents of sago starch-based composite system, a suitable alternative to gelatin can be produced with comparable properties and this could find potential application in pharmaceutical capsule industry.
26256336	6	40	theme	proposed	910:917	arg1	system					919:924	the proposed system	906:924	the proposed system	906:924	The viscoelastic moduli (G' and G") for the proposed system were lower than those of gelatin.
26256336	3	41	theme	system	472:477	arg1	properties					435:444	The rheological properties	419:444	The rheological properties of the proposed composite system	419:477	The rheological properties of the proposed composite system were measured and compared with gelatin as reference material.
26256336	3	41	theme	system	472:477	arg1	material					532:539	reference material	522:539	reference material	522:539	The rheological properties of the proposed composite system were measured and compared with gelatin as reference material.
26256336	5	42	from	transition	785:794	arg1	comparable					834:843	comparable	834:843	comparable	834:843	The solution viscosity at 50 °C and sol-gel transition of the proposed composite system were comparable to those of gelatin.
26256336	5	42	from	transition	785:794	arg1	viscosity					754:762	The solution viscosity	741:762	The solution viscosity at 50 °C and sol-gel transition of the proposed composite system	741:827	The solution viscosity at 50 °C and sol-gel transition of the proposed composite system were comparable to those of gelatin.
26256336	7	43	theme	constituents	1013:1024	arg1	manipulation					993:1004	manipulation	993:1004	manipulation of the constituents of sago starch-based composite system	993:1062	These results illustrate that by manipulation of the constituents of sago starch-based composite system, a suitable alternative to gelatin can be produced with comparable properties and this could find potential application in pharmaceutical capsule industry.
26256336	5	44	from	°C	770:771	arg1	comparable					834:843	comparable	834:843	comparable	834:843	The solution viscosity at 50 °C and sol-gel transition of the proposed composite system were comparable to those of gelatin.
26256336	5	44	from	°C	770:771	arg1	viscosity					754:762	The solution viscosity	741:762	The solution viscosity at 50 °C and sol-gel transition of the proposed composite system	741:827	The solution viscosity at 50 °C and sol-gel transition of the proposed composite system were comparable to those of gelatin.
26256336	2	45	theme	sago	345:348	arg1	starches					350:357	Dually modified (Hydrolyzed-Hydroxypropylated) sago starches	298:357	Dually modified (Hydrolyzed-Hydroxypropylated) sago starches	298:357	Dually modified (Hydrolyzed-Hydroxypropylated) sago starches were combined with κ-carrageenan (0.25, 0.5, 0.75, and 1%).
26256336	4	46	theme	sago	612:615	arg1	starch					617:622	Hydrolysed-hydroxypropylated sago starch	583:622	Hydrolysed-hydroxypropylated sago starch at 12h	583:629	Results show that combination of HHSS12 (Hydrolysed-hydroxypropylated sago starch at 12h) with 0.5% κ-carrageenan was comparable to gelatin rheological behavior in pharmaceutical capsule processing.
26256336	3	47	theme	composite	462:470	arg1	system					472:477	the proposed composite system	449:477	the proposed composite system	449:477	The rheological properties of the proposed composite system were measured and compared with gelatin as reference material.
26256336	3	48	theme	proposed	453:460	arg1	system					472:477	the proposed composite system	449:477	the proposed composite system	449:477	The rheological properties of the proposed composite system were measured and compared with gelatin as reference material.
26256336	0	49	theme	sago	70:73	arg1	starch					75:80	dually modified sago starch:	54:81	dually modified sago starch: Towards finding gelatin alternative for hard capsules	54:135	Effects of κ-carrageenan on rheological properties of dually modified sago starch: Towards finding gelatin alternative for hard capsules.
26256336	4	50	theme	gelatin	674:680	arg1	behavior					694:701	gelatin rheological behavior	674:701	gelatin rheological behavior in pharmaceutical capsule processing	674:738	Results show that combination of HHSS12 (Hydrolysed-hydroxypropylated sago starch at 12h) with 0.5% κ-carrageenan was comparable to gelatin rheological behavior in pharmaceutical capsule processing.
26256336	4	51	theme	HHSS12	575:580	arg1	combination					560:570	combination	560:570	combination of HHSS12 (Hydrolysed-hydroxypropylated sago starch at 12h) with 0.5% κ-carrageenan	560:654	Results show that combination of HHSS12 (Hydrolysed-hydroxypropylated sago starch at 12h) with 0.5% κ-carrageenan was comparable to gelatin rheological behavior in pharmaceutical capsule processing.
26256336	0	52	theme	modified	61:68	arg1	starch					75:80	dually modified sago starch:	54:81	dually modified sago starch: Towards finding gelatin alternative for hard capsules	54:135	Effects of κ-carrageenan on rheological properties of dually modified sago starch: Towards finding gelatin alternative for hard capsules.
26256336	4	53	with	combination	560:570	arg1	κ-carrageenan					642:654	0.5% κ-carrageenan	637:654	0.5% κ-carrageenan	637:654	Results show that combination of HHSS12 (Hydrolysed-hydroxypropylated sago starch at 12h) with 0.5% κ-carrageenan was comparable to gelatin rheological behavior in pharmaceutical capsule processing.
26256336	1	54	theme	sago	148:151	arg1	system					166:171	Composite sago starch-based system	138:171	Composite sago starch-based system	138:171	Composite sago starch-based system was developed and characterized with the aim to find an alternative to gelatin in the processing of pharmaceutical capsules.
26256336	4	55	from	behavior	694:701	arg1	processing					729:738	pharmaceutical capsule processing	706:738	pharmaceutical capsule processing	706:738	Results show that combination of HHSS12 (Hydrolysed-hydroxypropylated sago starch at 12h) with 0.5% κ-carrageenan was comparable to gelatin rheological behavior in pharmaceutical capsule processing.
28554545	4	0	theme	strategies	483:492	arg1	binding					504:510	covalent binding	495:510	covalent binding	495:510	A variety of CDs (unfunctionalized, positively charged and carboxymethylated) and three different coupling strategies (covalent binding, electrostatic adsorption and inclusion complexation) were systematically investigated for their ability to control the release of two antibiotic drugs, metronidazole and clofazimine.
28554545	4	0	theme	strategies	483:492	arg1	complexation					552:563	inclusion complexation	542:563	inclusion complexation	542:563	A variety of CDs (unfunctionalized, positively charged and carboxymethylated) and three different coupling strategies (covalent binding, electrostatic adsorption and inclusion complexation) were systematically investigated for their ability to control the release of two antibiotic drugs, metronidazole and clofazimine.
28554545	4	0	theme	strategies	483:492	arg1	variety					378:384	A variety	376:384	A variety of CDs (unfunctionalized, positively charged and carboxymethylated) and three different coupling strategies (covalent binding, electrostatic adsorption and inclusion complexation)	376:564	A variety of CDs (unfunctionalized, positively charged and carboxymethylated) and three different coupling strategies (covalent binding, electrostatic adsorption and inclusion complexation) were systematically investigated for their ability to control the release of two antibiotic drugs, metronidazole and clofazimine.
28554545	4	0	theme	strategies	483:492	arg1	CDs					389:391	CDs	389:391	CDs (unfunctionalized, positively charged and carboxymethylated)	389:452	A variety of CDs (unfunctionalized, positively charged and carboxymethylated) and three different coupling strategies (covalent binding, electrostatic adsorption and inclusion complexation) were systematically investigated for their ability to control the release of two antibiotic drugs, metronidazole and clofazimine.
28554545	4	0	theme	strategies	483:492	arg1	adsorption					527:536	electrostatic adsorption	513:536	electrostatic adsorption	513:536	A variety of CDs (unfunctionalized, positively charged and carboxymethylated) and three different coupling strategies (covalent binding, electrostatic adsorption and inclusion complexation) were systematically investigated for their ability to control the release of two antibiotic drugs, metronidazole and clofazimine.
28554545	4	0	theme	strategies	483:492	arg1	strategies					483:492	three different coupling strategies	458:492	three different coupling strategies (covalent binding, electrostatic adsorption and inclusion complexation)	458:564	A variety of CDs (unfunctionalized, positively charged and carboxymethylated) and three different coupling strategies (covalent binding, electrostatic adsorption and inclusion complexation) were systematically investigated for their ability to control the release of two antibiotic drugs, metronidazole and clofazimine.
28554545	10	1	theme	antibiotic	1498:1507	arg1	compounds					1509:1517	both hydrophobic and hydrophilic antibiotic compounds	1465:1517	both hydrophobic and hydrophilic antibiotic compounds with potential translation to triggered release formulation targeting bacterial infections in the colon and lower intestine	1465:1641	Using pharmaceutical manufacturing processes, our study shows that CD-MSP composites can retain both hydrophobic and hydrophilic antibiotic compounds with potential translation to triggered release formulation targeting bacterial infections in the colon and lower intestine.
28554545	6	2	theme	metronidazole-loaded-MSP	904:927	arg1	characterization					884:899	characterization	884:899	characterization	884:899	We report for the first time on the encapsulation and characterization of metronidazole-loaded-MSP.
28554545	6	2	theme	metronidazole-loaded-MSP	904:927	arg1	encapsulation					866:878	encapsulation	866:878	encapsulation	866:878	We report for the first time on the encapsulation and characterization of metronidazole-loaded-MSP.
28554545	7	3	theme	CD	935:936	arg1	strategy					946:953	Each CD coating strategy	930:953	Each CD coating strategy	930:953	Each CD coating strategy reduced the drug release rate in phosphate buffer compared to unmodified MSP (from 20% to 100% retained drug).
28554545	10	4	theme	release	1559:1565	arg1	formulation					1567:1577	triggered release formulation	1549:1577	triggered release formulation targeting bacterial infections in the colon and lower intestine	1549:1641	Using pharmaceutical manufacturing processes, our study shows that CD-MSP composites can retain both hydrophobic and hydrophilic antibiotic compounds with potential translation to triggered release formulation targeting bacterial infections in the colon and lower intestine.
28554545	10	5	theme	hydrophilic	1486:1496	arg1	compounds					1509:1517	both hydrophobic and hydrophilic antibiotic compounds	1465:1517	both hydrophobic and hydrophilic antibiotic compounds with potential translation to triggered release formulation targeting bacterial infections in the colon and lower intestine	1465:1641	Using pharmaceutical manufacturing processes, our study shows that CD-MSP composites can retain both hydrophobic and hydrophilic antibiotic compounds with potential translation to triggered release formulation targeting bacterial infections in the colon and lower intestine.
28554545	9	6	theme	novel	1205:1209	arg1	complex					1221:1227	the novel inclusion complex	1201:1227	the novel inclusion complex based on host/guest interaction between benzyl-modified silica surface and α-CD	1201:1307	In particular, the novel inclusion complex based on host/guest interaction between benzyl-modified silica surface and α-CD proved to be very effective (60-100% retained drug amount).
28554545	9	6	theme	novel	1205:1209	arg1	effective					1327:1335	effective	1327:1335	effective	1327:1335	In particular, the novel inclusion complex based on host/guest interaction between benzyl-modified silica surface and α-CD proved to be very effective (60-100% retained drug amount).
28554545	9	6	theme	novel	1205:1209	arg1	%					1344:1344	60-100%	1338:1344	60-100% retained drug amount	1338:1365	In particular, the novel inclusion complex based on host/guest interaction between benzyl-modified silica surface and α-CD proved to be very effective (60-100% retained drug amount).
28554545	10	7	theme	triggered	1549:1557	arg1	formulation					1567:1577	triggered release formulation	1549:1577	triggered release formulation targeting bacterial infections in the colon and lower intestine	1549:1641	Using pharmaceutical manufacturing processes, our study shows that CD-MSP composites can retain both hydrophobic and hydrophilic antibiotic compounds with potential translation to triggered release formulation targeting bacterial infections in the colon and lower intestine.
28554545	7	8	dep	%	1048:1048	arg1	to					1042:1043	to	1042:1043	to	1042:1043	Each CD coating strategy reduced the drug release rate in phosphate buffer compared to unmodified MSP (from 20% to 100% retained drug).
28554545	4	9	theme	covalent	495:502	arg1	strategies					483:492	three different coupling strategies	458:492	three different coupling strategies (covalent binding, electrostatic adsorption and inclusion complexation)	458:564	A variety of CDs (unfunctionalized, positively charged and carboxymethylated) and three different coupling strategies (covalent binding, electrostatic adsorption and inclusion complexation) were systematically investigated for their ability to control the release of two antibiotic drugs, metronidazole and clofazimine.
28554545	4	9	theme	covalent	495:502	arg1	binding					504:510	covalent binding	495:510	covalent binding	495:510	A variety of CDs (unfunctionalized, positively charged and carboxymethylated) and three different coupling strategies (covalent binding, electrostatic adsorption and inclusion complexation) were systematically investigated for their ability to control the release of two antibiotic drugs, metronidazole and clofazimine.
28554545	10	10	theme	lower	1627:1631	arg1	intestine					1633:1641	lower intestine	1627:1641	lower intestine	1627:1641	Using pharmaceutical manufacturing processes, our study shows that CD-MSP composites can retain both hydrophobic and hydrophilic antibiotic compounds with potential translation to triggered release formulation targeting bacterial infections in the colon and lower intestine.
28554545	7	11	theme	phosphate	988:996	arg1	buffer					998:1003	phosphate buffer	988:1003	phosphate buffer	988:1003	Each CD coating strategy reduced the drug release rate in phosphate buffer compared to unmodified MSP (from 20% to 100% retained drug).
28554545	10	12	theme	manufacturing	1390:1402	arg1	processes					1404:1412	pharmaceutical manufacturing processes	1375:1412	pharmaceutical manufacturing processes	1375:1412	Using pharmaceutical manufacturing processes, our study shows that CD-MSP composites can retain both hydrophobic and hydrophilic antibiotic compounds with potential translation to triggered release formulation targeting bacterial infections in the colon and lower intestine.
28554545	7	13	dep	retained	1050:1057	arg1	%					1048:1048	20% to 100%	1038:1048	20% to 100%	1038:1048	Each CD coating strategy reduced the drug release rate in phosphate buffer compared to unmodified MSP (from 20% to 100% retained drug).
28554545	2	14	theme	silica	164:169	arg1	MSPs					182:185	MSPs	182:185	MSPs	182:185	Cyclodextrins (CDs) and mesoporous silica particles (MSPs) have been combined as composite carriers for controlled antibiotic release.
28554545	2	14	theme	silica	164:169	arg1	particles					171:179	mesoporous silica particles	153:179	mesoporous silica particles (MSPs)	153:186	Cyclodextrins (CDs) and mesoporous silica particles (MSPs) have been combined as composite carriers for controlled antibiotic release.
28554545	2	14	theme	silica	164:169	arg1	carriers					220:227	composite carriers	210:227	composite carriers for controlled antibiotic release	210:261	Cyclodextrins (CDs) and mesoporous silica particles (MSPs) have been combined as composite carriers for controlled antibiotic release.
28554545	2	14	theme	silica	164:169	arg1	Cyclodextrins					129:141	Cyclodextrins	129:141	Cyclodextrins (CDs)	129:147	Cyclodextrins (CDs) and mesoporous silica particles (MSPs) have been combined as composite carriers for controlled antibiotic release.
28554545	10	15	theme	hydrophobic	1470:1480	arg1	compounds					1509:1517	both hydrophobic and hydrophilic antibiotic compounds	1465:1517	both hydrophobic and hydrophilic antibiotic compounds with potential translation to triggered release formulation targeting bacterial infections in the colon and lower intestine	1465:1641	Using pharmaceutical manufacturing processes, our study shows that CD-MSP composites can retain both hydrophobic and hydrophilic antibiotic compounds with potential translation to triggered release formulation targeting bacterial infections in the colon and lower intestine.
28554545	4	16	theme	CDs	389:391	arg1	binding					504:510	covalent binding	495:510	covalent binding	495:510	A variety of CDs (unfunctionalized, positively charged and carboxymethylated) and three different coupling strategies (covalent binding, electrostatic adsorption and inclusion complexation) were systematically investigated for their ability to control the release of two antibiotic drugs, metronidazole and clofazimine.
28554545	4	16	theme	CDs	389:391	arg1	complexation					552:563	inclusion complexation	542:563	inclusion complexation	542:563	A variety of CDs (unfunctionalized, positively charged and carboxymethylated) and three different coupling strategies (covalent binding, electrostatic adsorption and inclusion complexation) were systematically investigated for their ability to control the release of two antibiotic drugs, metronidazole and clofazimine.
28554545	4	16	theme	CDs	389:391	arg1	variety					378:384	A variety	376:384	A variety of CDs (unfunctionalized, positively charged and carboxymethylated) and three different coupling strategies (covalent binding, electrostatic adsorption and inclusion complexation)	376:564	A variety of CDs (unfunctionalized, positively charged and carboxymethylated) and three different coupling strategies (covalent binding, electrostatic adsorption and inclusion complexation) were systematically investigated for their ability to control the release of two antibiotic drugs, metronidazole and clofazimine.
28554545	4	16	theme	CDs	389:391	arg1	CDs					389:391	CDs	389:391	CDs (unfunctionalized, positively charged and carboxymethylated)	389:452	A variety of CDs (unfunctionalized, positively charged and carboxymethylated) and three different coupling strategies (covalent binding, electrostatic adsorption and inclusion complexation) were systematically investigated for their ability to control the release of two antibiotic drugs, metronidazole and clofazimine.
28554545	4	16	theme	CDs	389:391	arg1	adsorption					527:536	electrostatic adsorption	513:536	electrostatic adsorption	513:536	A variety of CDs (unfunctionalized, positively charged and carboxymethylated) and three different coupling strategies (covalent binding, electrostatic adsorption and inclusion complexation) were systematically investigated for their ability to control the release of two antibiotic drugs, metronidazole and clofazimine.
28554545	4	16	theme	CDs	389:391	arg1	strategies					483:492	three different coupling strategies	458:492	three different coupling strategies (covalent binding, electrostatic adsorption and inclusion complexation)	458:564	A variety of CDs (unfunctionalized, positively charged and carboxymethylated) and three different coupling strategies (covalent binding, electrostatic adsorption and inclusion complexation) were systematically investigated for their ability to control the release of two antibiotic drugs, metronidazole and clofazimine.
28554545	10	17	from	infections	1599:1608	arg1	colon					1617:1621	colon	1617:1621	colon	1617:1621	Using pharmaceutical manufacturing processes, our study shows that CD-MSP composites can retain both hydrophobic and hydrophilic antibiotic compounds with potential translation to triggered release formulation targeting bacterial infections in the colon and lower intestine.
28554545	10	17	from	infections	1599:1608	arg1	intestine					1633:1641	lower intestine	1627:1641	lower intestine	1627:1641	Using pharmaceutical manufacturing processes, our study shows that CD-MSP composites can retain both hydrophobic and hydrophilic antibiotic compounds with potential translation to triggered release formulation targeting bacterial infections in the colon and lower intestine.
28554545	2	18	theme	mesoporous	153:162	arg1	MSPs					182:185	MSPs	182:185	MSPs	182:185	Cyclodextrins (CDs) and mesoporous silica particles (MSPs) have been combined as composite carriers for controlled antibiotic release.
28554545	2	18	theme	mesoporous	153:162	arg1	particles					171:179	mesoporous silica particles	153:179	mesoporous silica particles (MSPs)	153:186	Cyclodextrins (CDs) and mesoporous silica particles (MSPs) have been combined as composite carriers for controlled antibiotic release.
28554545	2	18	theme	mesoporous	153:162	arg1	carriers					220:227	composite carriers	210:227	composite carriers for controlled antibiotic release	210:261	Cyclodextrins (CDs) and mesoporous silica particles (MSPs) have been combined as composite carriers for controlled antibiotic release.
28554545	2	18	theme	mesoporous	153:162	arg1	Cyclodextrins					129:141	Cyclodextrins	129:141	Cyclodextrins (CDs)	129:147	Cyclodextrins (CDs) and mesoporous silica particles (MSPs) have been combined as composite carriers for controlled antibiotic release.
28554545	8	19	theme	Covalent	1066:1073	arg1	binding					1075:1081	Covalent binding	1066:1081	Covalent binding	1066:1081	Covalent binding and inclusion complex approaches were significantly more effective than electrostatically adsorbed CD.
28554545	3	20	theme	drug	337:340	arg1	molecules					342:350	drug molecules	337:350	drug molecules	337:350	CDs were employed as "gatekeeper" agents and grafted onto MSPs to retain drug molecules inside the MSP carrier.
28554545	4	21	theme	coupling	474:481	arg1	adsorption					527:536	electrostatic adsorption	513:536	electrostatic adsorption	513:536	A variety of CDs (unfunctionalized, positively charged and carboxymethylated) and three different coupling strategies (covalent binding, electrostatic adsorption and inclusion complexation) were systematically investigated for their ability to control the release of two antibiotic drugs, metronidazole and clofazimine.
28554545	4	21	theme	coupling	474:481	arg1	complexation					552:563	inclusion complexation	542:563	inclusion complexation	542:563	A variety of CDs (unfunctionalized, positively charged and carboxymethylated) and three different coupling strategies (covalent binding, electrostatic adsorption and inclusion complexation) were systematically investigated for their ability to control the release of two antibiotic drugs, metronidazole and clofazimine.
28554545	4	21	theme	coupling	474:481	arg1	strategies					483:492	three different coupling strategies	458:492	three different coupling strategies (covalent binding, electrostatic adsorption and inclusion complexation)	458:564	A variety of CDs (unfunctionalized, positively charged and carboxymethylated) and three different coupling strategies (covalent binding, electrostatic adsorption and inclusion complexation) were systematically investigated for their ability to control the release of two antibiotic drugs, metronidazole and clofazimine.
28554545	4	21	theme	coupling	474:481	arg1	binding					504:510	covalent binding	495:510	covalent binding	495:510	A variety of CDs (unfunctionalized, positively charged and carboxymethylated) and three different coupling strategies (covalent binding, electrostatic adsorption and inclusion complexation) were systematically investigated for their ability to control the release of two antibiotic drugs, metronidazole and clofazimine.
28554545	10	22	theme	potential	1524:1532	arg1	translation					1534:1544	potential translation	1524:1544	potential translation to triggered release formulation targeting bacterial infections in the colon and lower intestine	1524:1641	Using pharmaceutical manufacturing processes, our study shows that CD-MSP composites can retain both hydrophobic and hydrophilic antibiotic compounds with potential translation to triggered release formulation targeting bacterial infections in the colon and lower intestine.
28554545	4	23	theme	drugs	658:662	arg1	release					632:638	the release	628:638	the release of two antibiotic drugs, metronidazole and clofazimine	628:693	A variety of CDs (unfunctionalized, positively charged and carboxymethylated) and three different coupling strategies (covalent binding, electrostatic adsorption and inclusion complexation) were systematically investigated for their ability to control the release of two antibiotic drugs, metronidazole and clofazimine.
28554545	0	24	theme	silica	24:29	arg1	composites					40:49	Cyclodextrin-mesoporous silica particle composites	0:49	Cyclodextrin-mesoporous silica particle composites for controlled antibiotic release	0:83	Cyclodextrin-mesoporous silica particle composites for controlled antibiotic release.
28554545	4	25	theme	different	464:472	arg1	adsorption					527:536	electrostatic adsorption	513:536	electrostatic adsorption	513:536	A variety of CDs (unfunctionalized, positively charged and carboxymethylated) and three different coupling strategies (covalent binding, electrostatic adsorption and inclusion complexation) were systematically investigated for their ability to control the release of two antibiotic drugs, metronidazole and clofazimine.
28554545	4	25	theme	different	464:472	arg1	complexation					552:563	inclusion complexation	542:563	inclusion complexation	542:563	A variety of CDs (unfunctionalized, positively charged and carboxymethylated) and three different coupling strategies (covalent binding, electrostatic adsorption and inclusion complexation) were systematically investigated for their ability to control the release of two antibiotic drugs, metronidazole and clofazimine.
28554545	4	25	theme	different	464:472	arg1	strategies					483:492	three different coupling strategies	458:492	three different coupling strategies (covalent binding, electrostatic adsorption and inclusion complexation)	458:564	A variety of CDs (unfunctionalized, positively charged and carboxymethylated) and three different coupling strategies (covalent binding, electrostatic adsorption and inclusion complexation) were systematically investigated for their ability to control the release of two antibiotic drugs, metronidazole and clofazimine.
28554545	4	25	theme	different	464:472	arg1	binding					504:510	covalent binding	495:510	covalent binding	495:510	A variety of CDs (unfunctionalized, positively charged and carboxymethylated) and three different coupling strategies (covalent binding, electrostatic adsorption and inclusion complexation) were systematically investigated for their ability to control the release of two antibiotic drugs, metronidazole and clofazimine.
28554545	9	26	theme	host/guest	1238:1247	arg1	interaction					1249:1259	host/guest interaction	1238:1259	host/guest interaction between benzyl-modified silica surface and α-CD	1238:1307	In particular, the novel inclusion complex based on host/guest interaction between benzyl-modified silica surface and α-CD proved to be very effective (60-100% retained drug amount).
28554545	1	27	theme	concept	97:103	arg1	proof					88:92	A proof	86:92	A proof of concept toward colon targeting	86:126	A proof of concept toward colon targeting.
28554545	0	28	theme	Cyclodextrin-mesoporous	0:22	arg1	composites					40:49	Cyclodextrin-mesoporous silica particle composites	0:49	Cyclodextrin-mesoporous silica particle composites for controlled antibiotic release	0:83	Cyclodextrin-mesoporous silica particle composites for controlled antibiotic release.
28554545	9	29	theme	inclusion	1211:1219	arg1	complex					1221:1227	the novel inclusion complex	1201:1227	the novel inclusion complex based on host/guest interaction between benzyl-modified silica surface and α-CD	1201:1307	In particular, the novel inclusion complex based on host/guest interaction between benzyl-modified silica surface and α-CD proved to be very effective (60-100% retained drug amount).
28554545	9	29	theme	inclusion	1211:1219	arg1	effective					1327:1335	effective	1327:1335	effective	1327:1335	In particular, the novel inclusion complex based on host/guest interaction between benzyl-modified silica surface and α-CD proved to be very effective (60-100% retained drug amount).
28554545	9	29	theme	inclusion	1211:1219	arg1	%					1344:1344	60-100%	1338:1344	60-100% retained drug amount	1338:1365	In particular, the novel inclusion complex based on host/guest interaction between benzyl-modified silica surface and α-CD proved to be very effective (60-100% retained drug amount).
28554545	7	30	theme	drug	967:970	arg1	rate					980:983	the drug release rate	963:983	the drug release rate in phosphate buffer	963:1003	Each CD coating strategy reduced the drug release rate in phosphate buffer compared to unmodified MSP (from 20% to 100% retained drug).
28554545	4	31	theme	inclusion	542:550	arg1	complexation					552:563	inclusion complexation	542:563	inclusion complexation	542:563	A variety of CDs (unfunctionalized, positively charged and carboxymethylated) and three different coupling strategies (covalent binding, electrostatic adsorption and inclusion complexation) were systematically investigated for their ability to control the release of two antibiotic drugs, metronidazole and clofazimine.
28554545	4	31	theme	inclusion	542:550	arg1	strategies					483:492	three different coupling strategies	458:492	three different coupling strategies (covalent binding, electrostatic adsorption and inclusion complexation)	458:564	A variety of CDs (unfunctionalized, positively charged and carboxymethylated) and three different coupling strategies (covalent binding, electrostatic adsorption and inclusion complexation) were systematically investigated for their ability to control the release of two antibiotic drugs, metronidazole and clofazimine.
28554545	8	32	theme	complex	1097:1103	arg1	approaches					1105:1114	inclusion complex approaches	1087:1114	inclusion complex approaches	1087:1114	Covalent binding and inclusion complex approaches were significantly more effective than electrostatically adsorbed CD.
28554545	7	33	theme	release	972:978	arg1	rate					980:983	the drug release rate	963:983	the drug release rate in phosphate buffer	963:1003	Each CD coating strategy reduced the drug release rate in phosphate buffer compared to unmodified MSP (from 20% to 100% retained drug).
28554545	1	34	theme	colon	112:116	arg1	targeting					118:126	colon targeting	112:126	colon targeting	112:126	A proof of concept toward colon targeting.
28554545	10	35	theme	CD-MSP	1436:1441	arg1	composites					1443:1452	CD-MSP composites	1436:1452	CD-MSP composites	1436:1452	Using pharmaceutical manufacturing processes, our study shows that CD-MSP composites can retain both hydrophobic and hydrophilic antibiotic compounds with potential translation to triggered release formulation targeting bacterial infections in the colon and lower intestine.
28554545	3	36	theme	MSP	363:365	arg1	carrier					367:373	the MSP carrier	359:373	the MSP carrier	359:373	CDs were employed as "gatekeeper" agents and grafted onto MSPs to retain drug molecules inside the MSP carrier.
28554545	8	37	theme	inclusion	1087:1095	arg1	approaches					1105:1114	inclusion complex approaches	1087:1114	inclusion complex approaches	1087:1114	Covalent binding and inclusion complex approaches were significantly more effective than electrostatically adsorbed CD.
28554545	9	38	theme	benzyl-modified	1269:1283	arg1	surface					1292:1298	benzyl-modified silica surface	1269:1298	benzyl-modified silica surface	1269:1298	In particular, the novel inclusion complex based on host/guest interaction between benzyl-modified silica surface and α-CD proved to be very effective (60-100% retained drug amount).
28554545	7	39	theme	retained	1050:1057	arg1	drug					1059:1062	20% to 100% retained drug	1038:1062	20% to 100% retained drug	1038:1062	Each CD coating strategy reduced the drug release rate in phosphate buffer compared to unmodified MSP (from 20% to 100% retained drug).
28554545	0	40	theme	controlled	55:64	arg1	release					77:83	controlled antibiotic release	55:83	controlled antibiotic release	55:83	Cyclodextrin-mesoporous silica particle composites for controlled antibiotic release.
28554545	5	41	contain	had	706:708	arg2	properties					750:759	significantly different physicochemical properties	710:759	significantly different physicochemical properties (metronidazole - small hydrophilic, clofazimine- large hydrophobic)	710:827	The drugs had significantly different physicochemical properties (metronidazole - small hydrophilic, clofazimine- large hydrophobic).
28554545	5	41	contain	had	706:708	arg1	drugs					700:704	The drugs	696:704	The drugs	696:704	The drugs had significantly different physicochemical properties (metronidazole - small hydrophilic, clofazimine- large hydrophobic).
28554545	5	42	theme	different	724:732	arg1	properties					750:759	significantly different physicochemical properties	710:759	significantly different physicochemical properties (metronidazole - small hydrophilic, clofazimine- large hydrophobic)	710:827	The drugs had significantly different physicochemical properties (metronidazole - small hydrophilic, clofazimine- large hydrophobic).
28554545	9	43	theme	silica	1285:1290	arg1	surface					1292:1298	benzyl-modified silica surface	1269:1298	benzyl-modified silica surface	1269:1298	In particular, the novel inclusion complex based on host/guest interaction between benzyl-modified silica surface and α-CD proved to be very effective (60-100% retained drug amount).
28554545	2	44	theme	controlled	233:242	arg1	release					255:261	controlled antibiotic release	233:261	controlled antibiotic release	233:261	Cyclodextrins (CDs) and mesoporous silica particles (MSPs) have been combined as composite carriers for controlled antibiotic release.
28554545	4	45	theme	antibiotic	647:656	arg1	clofazimine					683:693	clofazimine	683:693	clofazimine	683:693	A variety of CDs (unfunctionalized, positively charged and carboxymethylated) and three different coupling strategies (covalent binding, electrostatic adsorption and inclusion complexation) were systematically investigated for their ability to control the release of two antibiotic drugs, metronidazole and clofazimine.
28554545	4	45	theme	antibiotic	647:656	arg1	metronidazole					665:677	metronidazole	665:677	metronidazole	665:677	A variety of CDs (unfunctionalized, positively charged and carboxymethylated) and three different coupling strategies (covalent binding, electrostatic adsorption and inclusion complexation) were systematically investigated for their ability to control the release of two antibiotic drugs, metronidazole and clofazimine.
28554545	4	45	theme	antibiotic	647:656	arg1	drugs					658:662	two antibiotic drugs	643:662	two antibiotic drugs	643:662	A variety of CDs (unfunctionalized, positively charged and carboxymethylated) and three different coupling strategies (covalent binding, electrostatic adsorption and inclusion complexation) were systematically investigated for their ability to control the release of two antibiotic drugs, metronidazole and clofazimine.
28554545	0	46	theme	particle	31:38	arg1	composites					40:49	Cyclodextrin-mesoporous silica particle composites	0:49	Cyclodextrin-mesoporous silica particle composites for controlled antibiotic release	0:83	Cyclodextrin-mesoporous silica particle composites for controlled antibiotic release.
28554545	4	47	theme	electrostatic	513:525	arg1	adsorption					527:536	electrostatic adsorption	513:536	electrostatic adsorption	513:536	A variety of CDs (unfunctionalized, positively charged and carboxymethylated) and three different coupling strategies (covalent binding, electrostatic adsorption and inclusion complexation) were systematically investigated for their ability to control the release of two antibiotic drugs, metronidazole and clofazimine.
28554545	4	47	theme	electrostatic	513:525	arg1	strategies					483:492	three different coupling strategies	458:492	three different coupling strategies (covalent binding, electrostatic adsorption and inclusion complexation)	458:564	A variety of CDs (unfunctionalized, positively charged and carboxymethylated) and three different coupling strategies (covalent binding, electrostatic adsorption and inclusion complexation) were systematically investigated for their ability to control the release of two antibiotic drugs, metronidazole and clofazimine.
28554545	5	48	theme	physicochemical	734:748	arg1	properties					750:759	significantly different physicochemical properties	710:759	significantly different physicochemical properties (metronidazole - small hydrophilic, clofazimine- large hydrophobic)	710:827	The drugs had significantly different physicochemical properties (metronidazole - small hydrophilic, clofazimine- large hydrophobic).
28554545	10	49	theme	pharmaceutical	1375:1388	arg1	processes					1404:1412	pharmaceutical manufacturing processes	1375:1412	pharmaceutical manufacturing processes	1375:1412	Using pharmaceutical manufacturing processes, our study shows that CD-MSP composites can retain both hydrophobic and hydrophilic antibiotic compounds with potential translation to triggered release formulation targeting bacterial infections in the colon and lower intestine.
28554545	10	50	dep	colon	1617:1621	arg1	the					1613:1615	the	1613:1615	the	1613:1615	Using pharmaceutical manufacturing processes, our study shows that CD-MSP composites can retain both hydrophobic and hydrophilic antibiotic compounds with potential translation to triggered release formulation targeting bacterial infections in the colon and lower intestine.
28554545	6	51	dep	encapsulation	866:878	arg1	the					862:864	the	862:864	the	862:864	We report for the first time on the encapsulation and characterization of metronidazole-loaded-MSP.
28554545	5	52	dep	metronidazole	762:774	arg1	hydrophilic					784:794	hydrophilic	784:794	hydrophilic	784:794	The drugs had significantly different physicochemical properties (metronidazole - small hydrophilic, clofazimine- large hydrophobic).
28554545	9	53	theme	drug	1355:1358	arg1	amount					1360:1365	drug amount	1355:1365	drug amount	1355:1365	In particular, the novel inclusion complex based on host/guest interaction between benzyl-modified silica surface and α-CD proved to be very effective (60-100% retained drug amount).
28554545	0	54	theme	antibiotic	66:75	arg1	release					77:83	controlled antibiotic release	55:83	controlled antibiotic release	55:83	Cyclodextrin-mesoporous silica particle composites for controlled antibiotic release.
28554545	2	55	theme	composite	210:218	arg1	particles					171:179	mesoporous silica particles	153:179	mesoporous silica particles (MSPs)	153:186	Cyclodextrins (CDs) and mesoporous silica particles (MSPs) have been combined as composite carriers for controlled antibiotic release.
28554545	2	55	theme	composite	210:218	arg1	carriers					220:227	composite carriers	210:227	composite carriers for controlled antibiotic release	210:261	Cyclodextrins (CDs) and mesoporous silica particles (MSPs) have been combined as composite carriers for controlled antibiotic release.
28554545	2	55	theme	composite	210:218	arg1	Cyclodextrins					129:141	Cyclodextrins	129:141	Cyclodextrins (CDs)	129:147	Cyclodextrins (CDs) and mesoporous silica particles (MSPs) have been combined as composite carriers for controlled antibiotic release.
28554545	9	56	dep	effective	1327:1335	arg1	complex					1221:1227	the novel inclusion complex	1201:1227	the novel inclusion complex based on host/guest interaction between benzyl-modified silica surface and α-CD	1201:1307	In particular, the novel inclusion complex based on host/guest interaction between benzyl-modified silica surface and α-CD proved to be very effective (60-100% retained drug amount).
28554545	9	56	dep	effective	1327:1335	arg1	effective					1327:1335	effective	1327:1335	effective	1327:1335	In particular, the novel inclusion complex based on host/guest interaction between benzyl-modified silica surface and α-CD proved to be very effective (60-100% retained drug amount).
28554545	9	56	dep	effective	1327:1335	arg1	%					1344:1344	60-100%	1338:1344	60-100% retained drug amount	1338:1365	In particular, the novel inclusion complex based on host/guest interaction between benzyl-modified silica surface and α-CD proved to be very effective (60-100% retained drug amount).
28554545	4	57	dep	strategies	483:492	arg1	adsorption					527:536	electrostatic adsorption	513:536	electrostatic adsorption	513:536	A variety of CDs (unfunctionalized, positively charged and carboxymethylated) and three different coupling strategies (covalent binding, electrostatic adsorption and inclusion complexation) were systematically investigated for their ability to control the release of two antibiotic drugs, metronidazole and clofazimine.
28554545	4	57	dep	strategies	483:492	arg1	complexation					552:563	inclusion complexation	542:563	inclusion complexation	542:563	A variety of CDs (unfunctionalized, positively charged and carboxymethylated) and three different coupling strategies (covalent binding, electrostatic adsorption and inclusion complexation) were systematically investigated for their ability to control the release of two antibiotic drugs, metronidazole and clofazimine.
28554545	4	57	dep	strategies	483:492	arg1	strategies					483:492	three different coupling strategies	458:492	three different coupling strategies (covalent binding, electrostatic adsorption and inclusion complexation)	458:564	A variety of CDs (unfunctionalized, positively charged and carboxymethylated) and three different coupling strategies (covalent binding, electrostatic adsorption and inclusion complexation) were systematically investigated for their ability to control the release of two antibiotic drugs, metronidazole and clofazimine.
28554545	4	57	dep	strategies	483:492	arg1	binding					504:510	covalent binding	495:510	covalent binding	495:510	A variety of CDs (unfunctionalized, positively charged and carboxymethylated) and three different coupling strategies (covalent binding, electrostatic adsorption and inclusion complexation) were systematically investigated for their ability to control the release of two antibiotic drugs, metronidazole and clofazimine.
28554545	5	58	dep	properties	750:759	arg1	metronidazole					762:774	metronidazole	762:774	metronidazole	762:774	The drugs had significantly different physicochemical properties (metronidazole - small hydrophilic, clofazimine- large hydrophobic).
28554545	5	58	dep	properties	750:759	arg1	hydrophobic					816:826	hydrophobic	816:826	hydrophobic	816:826	The drugs had significantly different physicochemical properties (metronidazole - small hydrophilic, clofazimine- large hydrophobic).
28554545	7	59	theme	%	1040:1040	arg1	%					1048:1048	20% to 100%	1038:1048	20% to 100%	1038:1048	Each CD coating strategy reduced the drug release rate in phosphate buffer compared to unmodified MSP (from 20% to 100% retained drug).
28554545	7	60	from	rate	980:983	arg1	buffer					998:1003	phosphate buffer	988:1003	phosphate buffer	988:1003	Each CD coating strategy reduced the drug release rate in phosphate buffer compared to unmodified MSP (from 20% to 100% retained drug).
28554545	3	61	theme	"	296:296	arg1	agents					298:303	"gatekeeper" agents	285:303	"gatekeeper" agents	285:303	CDs were employed as "gatekeeper" agents and grafted onto MSPs to retain drug molecules inside the MSP carrier.
28554545	3	61	theme	"	296:296	arg1	CDs					264:266	CDs	264:266	CDs	264:266	CDs were employed as "gatekeeper" agents and grafted onto MSPs to retain drug molecules inside the MSP carrier.
28554545	10	62	with	compounds	1509:1517	arg1	translation					1534:1544	potential translation	1524:1544	potential translation to triggered release formulation targeting bacterial infections in the colon and lower intestine	1524:1641	Using pharmaceutical manufacturing processes, our study shows that CD-MSP composites can retain both hydrophobic and hydrophilic antibiotic compounds with potential translation to triggered release formulation targeting bacterial infections in the colon and lower intestine.
28554545	7	63	theme	coating	938:944	arg1	strategy					946:953	Each CD coating strategy	930:953	Each CD coating strategy	930:953	Each CD coating strategy reduced the drug release rate in phosphate buffer compared to unmodified MSP (from 20% to 100% retained drug).
28554545	8	64	theme	adsorbed	1173:1180	arg1	CD					1182:1183	electrostatically adsorbed CD	1155:1183	electrostatically adsorbed CD	1155:1183	Covalent binding and inclusion complex approaches were significantly more effective than electrostatically adsorbed CD.
28554545	6	65	theme	first	848:852	arg1	time					854:857	the first time	844:857	the first time	844:857	We report for the first time on the encapsulation and characterization of metronidazole-loaded-MSP.
28554545	10	66	theme	bacterial	1589:1597	arg1	infections					1599:1608	bacterial infections	1589:1608	bacterial infections in the colon and lower intestine	1589:1641	Using pharmaceutical manufacturing processes, our study shows that CD-MSP composites can retain both hydrophobic and hydrophilic antibiotic compounds with potential translation to triggered release formulation targeting bacterial infections in the colon and lower intestine.
28554545	7	67	from	drug	1059:1062	arg1	MSP					1028:1030	unmodified MSP	1017:1030	unmodified MSP (from 20% to 100% retained drug)	1017:1063	Each CD coating strategy reduced the drug release rate in phosphate buffer compared to unmodified MSP (from 20% to 100% retained drug).
28554545	2	68	theme	antibiotic	244:253	arg1	release					255:261	controlled antibiotic release	233:261	controlled antibiotic release	233:261	Cyclodextrins (CDs) and mesoporous silica particles (MSPs) have been combined as composite carriers for controlled antibiotic release.
28554545	7	69	theme	unmodified	1017:1026	arg1	MSP					1028:1030	unmodified MSP	1017:1030	unmodified MSP (from 20% to 100% retained drug)	1017:1063	Each CD coating strategy reduced the drug release rate in phosphate buffer compared to unmodified MSP (from 20% to 100% retained drug).
28554545	4	70	dep	CDs	389:391	arg1	carboxymethylated					435:451	carboxymethylated	435:451	carboxymethylated	435:451	A variety of CDs (unfunctionalized, positively charged and carboxymethylated) and three different coupling strategies (covalent binding, electrostatic adsorption and inclusion complexation) were systematically investigated for their ability to control the release of two antibiotic drugs, metronidazole and clofazimine.
28554545	4	70	dep	CDs	389:391	arg1	unfunctionalized					394:409	unfunctionalized	394:409	unfunctionalized	394:409	A variety of CDs (unfunctionalized, positively charged and carboxymethylated) and three different coupling strategies (covalent binding, electrostatic adsorption and inclusion complexation) were systematically investigated for their ability to control the release of two antibiotic drugs, metronidazole and clofazimine.
28554545	4	70	dep	CDs	389:391	arg1	charged					423:429	charged	423:429	charged	423:429	A variety of CDs (unfunctionalized, positively charged and carboxymethylated) and three different coupling strategies (covalent binding, electrostatic adsorption and inclusion complexation) were systematically investigated for their ability to control the release of two antibiotic drugs, metronidazole and clofazimine.
28246387	0	0	theme	Components	91:100	arg1	Regulation					62:71	Coordinated Regulation	50:71	Coordinated Regulation of Secondary Wall Components in Tobacco	50:111	Functional Characterization of Populus PsnSHN2 in Coordinated Regulation of Secondary Wall Components in Tobacco.
28246387	8	1	theme	wall	1563:1566	arg1	formation					1568:1576	secondary wall formation	1553:1576	secondary wall formation	1553:1576	Taken together, our results suggest PsnSHN2 coordinately regulate secondary wall formation through selective up/down-regulation of its downstream transcription factors that control secondary wall formation.
28246387	5	2	theme	wall	863:866	arg1	formation					868:876	secondary wall formation	853:876	secondary wall formation	853:876	Overexpression of PsnSHN2 in tobacco significantly altered the expression of both transcription factors and biosynthesis genes involved in secondary wall formation, leading to the thickened secondary walls and the changed cell wall composition.
28246387	6	3	theme	hemicellulose	1030:1042	arg1	contents					1004:1011	the contents	1000:1011	the contents of cellulose and hemicellulose that increased 37% and 28%, respectively	1000:1083	The most significant changes occurred in the contents of cellulose and hemicellulose that increased 37% and 28%, respectively, whereas the content of lignin that decreased 34%.
28246387	0	4	theme	Wall	86:89	arg1	Components					91:100	Secondary Wall Components	76:100	Secondary Wall Components	76:100	Functional Characterization of Populus PsnSHN2 in Coordinated Regulation of Secondary Wall Components in Tobacco.
28246387	0	5	from	Characterization	11:26	arg1	Regulation					62:71	Coordinated Regulation	50:71	Coordinated Regulation of Secondary Wall Components in Tobacco	50:111	Functional Characterization of Populus PsnSHN2 in Coordinated Regulation of Secondary Wall Components in Tobacco.
28246387	8	6	theme	selective	1471:1479	arg1	up/down-regulation					1481:1498	selective up/down-regulation	1471:1498	selective up/down-regulation of its downstream transcription factors that control secondary wall formation	1471:1576	Taken together, our results suggest PsnSHN2 coordinately regulate secondary wall formation through selective up/down-regulation of its downstream transcription factors that control secondary wall formation.
28246387	2	7	theme	them	343:346	arg1	none					335:338	none	335:338	none of them	335:346	Although a number of transcription factors have been linked to the regulation of wood formation process, none of them has been demonstrated to be a higher hierarchical regulator that coordinately regulates secondary wall biosynthesis genes.
28246387	2	7	theme	them	343:346	arg1	regulator					398:406	a higher hierarchical regulator	376:406	a higher hierarchical regulator that coordinately regulates secondary wall biosynthesis genes	376:468	Although a number of transcription factors have been linked to the regulation of wood formation process, none of them has been demonstrated to be a higher hierarchical regulator that coordinately regulates secondary wall biosynthesis genes.
28246387	7	8	theme	transcription	1207:1219	arg1	factors					1221:1227	transcription factors	1207:1227	transcription factors involved in secondary wall biosynthesis	1207:1267	Furthermore, PsnSHN2 activated or repressed the promoter activities of transcription factors involved in secondary wall biosynthesis and bound to five cis-acting elements enriched in the promoter regions of these transcription factors.
28246387	7	9	theme	transcription	1349:1361	arg1	factors					1363:1369	these transcription factors	1343:1369	these transcription factors	1343:1369	Furthermore, PsnSHN2 activated or repressed the promoter activities of transcription factors involved in secondary wall biosynthesis and bound to five cis-acting elements enriched in the promoter regions of these transcription factors.
28246387	8	10	theme	wall	1448:1451	arg1	formation					1453:1461	secondary wall formation	1438:1461	secondary wall formation	1438:1461	Taken together, our results suggest PsnSHN2 coordinately regulate secondary wall formation through selective up/down-regulation of its downstream transcription factors that control secondary wall formation.
28246387	3	11	theme	AP2/ERF	549:555	arg1	factor					576:581	the Arabidopsis AP2/ERF type transcription factor	533:581	the Arabidopsis AP2/ERF type transcription factor	533:581	Here, we identified a Populus gene, PsnSHN2, a counterpart of the Arabidopsis AP2/ERF type transcription factor, SHINE2.
28246387	6	12	theme	cellulose	1016:1024	arg1	contents					1004:1011	the contents	1000:1011	the contents of cellulose and hemicellulose that increased 37% and 28%, respectively	1000:1083	The most significant changes occurred in the contents of cellulose and hemicellulose that increased 37% and 28%, respectively, whereas the content of lignin that decreased 34%.
28246387	3	13	theme	type	557:560	arg1	factor					576:581	the Arabidopsis AP2/ERF type transcription factor	533:581	the Arabidopsis AP2/ERF type transcription factor	533:581	Here, we identified a Populus gene, PsnSHN2, a counterpart of the Arabidopsis AP2/ERF type transcription factor, SHINE2.
28246387	0	14	from	Regulation	62:71	arg1	Tobacco					105:111	Tobacco	105:111	Tobacco	105:111	Functional Characterization of Populus PsnSHN2 in Coordinated Regulation of Secondary Wall Components in Tobacco.
28246387	1	15	theme	biological	134:143	arg1	formation					119:127	Wood formation	114:127	Wood formation	114:127	Wood formation is a biological process during which the most abundant lignocellulosic biomass on earth is produced.
28246387	1	15	theme	biological	134:143	arg1	process					145:151	a biological process	132:151	a biological process during which the most abundant lignocellulosic biomass on earth is produced	132:227	Wood formation is a biological process during which the most abundant lignocellulosic biomass on earth is produced.
28246387	4	16	theme	transcriptional	687:701	arg1	activator					703:711	a high hierarchical transcriptional activator	667:711	a high hierarchical transcriptional activator	667:711	PsnSHN2 is predominantly expressed in xylem tissues and acted evidently as a high hierarchical transcriptional activator.
28246387	5	17	theme	genes	835:839	arg1	expression					777:786	the expression	773:786	the expression of both transcription factors and biosynthesis genes involved in secondary wall formation	773:876	Overexpression of PsnSHN2 in tobacco significantly altered the expression of both transcription factors and biosynthesis genes involved in secondary wall formation, leading to the thickened secondary walls and the changed cell wall composition.
28246387	5	18	from	Overexpression	714:727	arg1	tobacco					743:749	tobacco	743:749	tobacco	743:749	Overexpression of PsnSHN2 in tobacco significantly altered the expression of both transcription factors and biosynthesis genes involved in secondary wall formation, leading to the thickened secondary walls and the changed cell wall composition.
28246387	5	19	theme	thickened	894:902	arg1	walls					914:918	the thickened secondary walls	890:918	the thickened secondary walls	890:918	Overexpression of PsnSHN2 in tobacco significantly altered the expression of both transcription factors and biosynthesis genes involved in secondary wall formation, leading to the thickened secondary walls and the changed cell wall composition.
28246387	2	20	theme	higher	378:383	arg1	none					335:338	none	335:338	none of them	335:346	Although a number of transcription factors have been linked to the regulation of wood formation process, none of them has been demonstrated to be a higher hierarchical regulator that coordinately regulates secondary wall biosynthesis genes.
28246387	2	20	theme	higher	378:383	arg1	regulator					398:406	a higher hierarchical regulator	376:406	a higher hierarchical regulator that coordinately regulates secondary wall biosynthesis genes	376:468	Although a number of transcription factors have been linked to the regulation of wood formation process, none of them has been demonstrated to be a higher hierarchical regulator that coordinately regulates secondary wall biosynthesis genes.
28246387	5	21	theme	biosynthesis	822:833	arg1	genes					835:839	biosynthesis genes	822:839	biosynthesis genes	822:839	Overexpression of PsnSHN2 in tobacco significantly altered the expression of both transcription factors and biosynthesis genes involved in secondary wall formation, leading to the thickened secondary walls and the changed cell wall composition.
28246387	5	22	theme	changed	928:934	arg1	composition					946:956	the changed cell wall composition	924:956	the changed cell wall composition	924:956	Overexpression of PsnSHN2 in tobacco significantly altered the expression of both transcription factors and biosynthesis genes involved in secondary wall formation, leading to the thickened secondary walls and the changed cell wall composition.
28246387	0	23	theme	Functional	0:9	arg1	Characterization					11:26	Functional Characterization	0:26	Functional Characterization of Populus PsnSHN2 in Coordinated Regulation of Secondary Wall Components in Tobacco.	0:112	Functional Characterization of Populus PsnSHN2 in Coordinated Regulation of Secondary Wall Components in Tobacco.
28246387	1	24	theme	abundant	175:182	arg1	biomass					200:206	the most abundant lignocellulosic biomass	166:206	the most abundant lignocellulosic biomass on earth	166:215	Wood formation is a biological process during which the most abundant lignocellulosic biomass on earth is produced.
28246387	2	25	theme	biosynthesis	451:462	arg1	genes					464:468	secondary wall biosynthesis genes	436:468	secondary wall biosynthesis genes	436:468	Although a number of transcription factors have been linked to the regulation of wood formation process, none of them has been demonstrated to be a higher hierarchical regulator that coordinately regulates secondary wall biosynthesis genes.
28246387	7	26	theme	secondary	1241:1249	arg1	biosynthesis					1256:1267	secondary wall biosynthesis	1241:1267	secondary wall biosynthesis	1241:1267	Furthermore, PsnSHN2 activated or repressed the promoter activities of transcription factors involved in secondary wall biosynthesis and bound to five cis-acting elements enriched in the promoter regions of these transcription factors.
28246387	2	27	theme	formation	316:324	arg1	process					326:332	wood formation process	311:332	wood formation process	311:332	Although a number of transcription factors have been linked to the regulation of wood formation process, none of them has been demonstrated to be a higher hierarchical regulator that coordinately regulates secondary wall biosynthesis genes.
28246387	7	28	theme	cis-acting	1287:1296	arg1	elements					1298:1305	five cis-acting elements	1282:1305	five cis-acting elements enriched in the promoter regions of these transcription factors	1282:1369	Furthermore, PsnSHN2 activated or repressed the promoter activities of transcription factors involved in secondary wall biosynthesis and bound to five cis-acting elements enriched in the promoter regions of these transcription factors.
28246387	1	29	theme	lignocellulosic	184:198	arg1	biomass					200:206	the most abundant lignocellulosic biomass	166:206	the most abundant lignocellulosic biomass on earth	166:215	Wood formation is a biological process during which the most abundant lignocellulosic biomass on earth is produced.
28246387	2	30	theme	wall	446:449	arg1	genes					464:468	secondary wall biosynthesis genes	436:468	secondary wall biosynthesis genes	436:468	Although a number of transcription factors have been linked to the regulation of wood formation process, none of them has been demonstrated to be a higher hierarchical regulator that coordinately regulates secondary wall biosynthesis genes.
28246387	2	31	theme	wood	311:314	arg1	process					326:332	wood formation process	311:332	wood formation process	311:332	Although a number of transcription factors have been linked to the regulation of wood formation process, none of them has been demonstrated to be a higher hierarchical regulator that coordinately regulates secondary wall biosynthesis genes.
28246387	5	32	theme	secondary	904:912	arg1	walls					914:918	the thickened secondary walls	890:918	the thickened secondary walls	890:918	Overexpression of PsnSHN2 in tobacco significantly altered the expression of both transcription factors and biosynthesis genes involved in secondary wall formation, leading to the thickened secondary walls and the changed cell wall composition.
28246387	0	33	theme	Populus	31:37	arg1	PsnSHN2					39:45	Populus PsnSHN2	31:45	Populus PsnSHN2	31:45	Functional Characterization of Populus PsnSHN2 in Coordinated Regulation of Secondary Wall Components in Tobacco.
28246387	3	34	theme	Arabidopsis	537:547	arg1	factor					576:581	the Arabidopsis AP2/ERF type transcription factor	533:581	the Arabidopsis AP2/ERF type transcription factor	533:581	Here, we identified a Populus gene, PsnSHN2, a counterpart of the Arabidopsis AP2/ERF type transcription factor, SHINE2.
28246387	4	35	theme	xylem	630:634	arg1	tissues					636:642	xylem tissues	630:642	xylem tissues	630:642	PsnSHN2 is predominantly expressed in xylem tissues and acted evidently as a high hierarchical transcriptional activator.
28246387	3	36	theme	transcription	562:574	arg1	factor					576:581	the Arabidopsis AP2/ERF type transcription factor	533:581	the Arabidopsis AP2/ERF type transcription factor	533:581	Here, we identified a Populus gene, PsnSHN2, a counterpart of the Arabidopsis AP2/ERF type transcription factor, SHINE2.
28246387	1	37	theme	Wood	114:117	arg1	formation					119:127	Wood formation	114:127	Wood formation	114:127	Wood formation is a biological process during which the most abundant lignocellulosic biomass on earth is produced.
28246387	1	37	theme	Wood	114:117	arg1	process					145:151	a biological process	132:151	a biological process during which the most abundant lignocellulosic biomass on earth is produced	132:227	Wood formation is a biological process during which the most abundant lignocellulosic biomass on earth is produced.
28246387	5	38	theme	transcription	796:808	arg1	factors					810:816	transcription factors	796:816	transcription factors	796:816	Overexpression of PsnSHN2 in tobacco significantly altered the expression of both transcription factors and biosynthesis genes involved in secondary wall formation, leading to the thickened secondary walls and the changed cell wall composition.
28246387	5	39	theme	wall	941:944	arg1	composition					946:956	the changed cell wall composition	924:956	the changed cell wall composition	924:956	Overexpression of PsnSHN2 in tobacco significantly altered the expression of both transcription factors and biosynthesis genes involved in secondary wall formation, leading to the thickened secondary walls and the changed cell wall composition.
28246387	7	40	theme	promoter	1184:1191	arg1	activities					1193:1202	the promoter activities	1180:1202	the promoter activities of transcription factors involved in secondary wall biosynthesis	1180:1267	Furthermore, PsnSHN2 activated or repressed the promoter activities of transcription factors involved in secondary wall biosynthesis and bound to five cis-acting elements enriched in the promoter regions of these transcription factors.
28246387	5	41	theme	PsnSHN2	732:738	arg1	Overexpression					714:727	Overexpression	714:727	Overexpression of PsnSHN2 in tobacco	714:749	Overexpression of PsnSHN2 in tobacco significantly altered the expression of both transcription factors and biosynthesis genes involved in secondary wall formation, leading to the thickened secondary walls and the changed cell wall composition.
28246387	0	42	theme	PsnSHN2	39:45	arg1	Characterization					11:26	Functional Characterization	0:26	Functional Characterization of Populus PsnSHN2 in Coordinated Regulation of Secondary Wall Components in Tobacco.	0:112	Functional Characterization of Populus PsnSHN2 in Coordinated Regulation of Secondary Wall Components in Tobacco.
28246387	5	43	theme	factors	810:816	arg1	expression					777:786	the expression	773:786	the expression of both transcription factors and biosynthesis genes involved in secondary wall formation	773:876	Overexpression of PsnSHN2 in tobacco significantly altered the expression of both transcription factors and biosynthesis genes involved in secondary wall formation, leading to the thickened secondary walls and the changed cell wall composition.
28246387	8	44	theme	transcription	1518:1530	arg1	factors					1532:1538	its downstream transcription factors	1503:1538	its downstream transcription factors that control secondary wall formation	1503:1576	Taken together, our results suggest PsnSHN2 coordinately regulate secondary wall formation through selective up/down-regulation of its downstream transcription factors that control secondary wall formation.
28246387	2	45	theme	secondary	436:444	arg1	genes					464:468	secondary wall biosynthesis genes	436:468	secondary wall biosynthesis genes	436:468	Although a number of transcription factors have been linked to the regulation of wood formation process, none of them has been demonstrated to be a higher hierarchical regulator that coordinately regulates secondary wall biosynthesis genes.
28246387	2	46	theme	hierarchical	385:396	arg1	none					335:338	none	335:338	none of them	335:346	Although a number of transcription factors have been linked to the regulation of wood formation process, none of them has been demonstrated to be a higher hierarchical regulator that coordinately regulates secondary wall biosynthesis genes.
28246387	2	46	theme	hierarchical	385:396	arg1	regulator					398:406	a higher hierarchical regulator	376:406	a higher hierarchical regulator that coordinately regulates secondary wall biosynthesis genes	376:468	Although a number of transcription factors have been linked to the regulation of wood formation process, none of them has been demonstrated to be a higher hierarchical regulator that coordinately regulates secondary wall biosynthesis genes.
28246387	6	47	theme	significant	968:978	arg1	changes					980:986	The most significant changes	959:986	The most significant changes	959:986	The most significant changes occurred in the contents of cellulose and hemicellulose that increased 37% and 28%, respectively, whereas the content of lignin that decreased 34%.
28246387	8	48	theme	downstream	1507:1516	arg1	factors					1532:1538	its downstream transcription factors	1503:1538	its downstream transcription factors that control secondary wall formation	1503:1576	Taken together, our results suggest PsnSHN2 coordinately regulate secondary wall formation through selective up/down-regulation of its downstream transcription factors that control secondary wall formation.
28246387	3	49	theme	factor	576:581	arg1	SHINE2					584:589	SHINE2	584:589	SHINE2	584:589	Here, we identified a Populus gene, PsnSHN2, a counterpart of the Arabidopsis AP2/ERF type transcription factor, SHINE2.
28246387	3	49	theme	factor	576:581	arg1	counterpart					518:528	a counterpart	516:528	a counterpart of the Arabidopsis AP2/ERF type transcription factor	516:581	Here, we identified a Populus gene, PsnSHN2, a counterpart of the Arabidopsis AP2/ERF type transcription factor, SHINE2.
28246387	3	49	theme	factor	576:581	arg1	gene					501:504	a Populus gene	491:504	a Populus gene	491:504	Here, we identified a Populus gene, PsnSHN2, a counterpart of the Arabidopsis AP2/ERF type transcription factor, SHINE2.
28246387	5	50	theme	cell	936:939	arg1	composition					946:956	the changed cell wall composition	924:956	the changed cell wall composition	924:956	Overexpression of PsnSHN2 in tobacco significantly altered the expression of both transcription factors and biosynthesis genes involved in secondary wall formation, leading to the thickened secondary walls and the changed cell wall composition.
28246387	0	51	theme	Coordinated	50:60	arg1	Regulation					62:71	Coordinated Regulation	50:71	Coordinated Regulation of Secondary Wall Components in Tobacco	50:111	Functional Characterization of Populus PsnSHN2 in Coordinated Regulation of Secondary Wall Components in Tobacco.
28246387	6	52	theme	most	963:966	arg1	changes					980:986	The most significant changes	959:986	The most significant changes	959:986	The most significant changes occurred in the contents of cellulose and hemicellulose that increased 37% and 28%, respectively, whereas the content of lignin that decreased 34%.
28246387	8	53	theme	secondary	1438:1446	arg1	formation					1453:1461	secondary wall formation	1438:1461	secondary wall formation	1438:1461	Taken together, our results suggest PsnSHN2 coordinately regulate secondary wall formation through selective up/down-regulation of its downstream transcription factors that control secondary wall formation.
28246387	7	54	theme	factors	1363:1369	arg1	regions					1332:1338	the promoter regions	1319:1338	the promoter regions of these transcription factors	1319:1369	Furthermore, PsnSHN2 activated or repressed the promoter activities of transcription factors involved in secondary wall biosynthesis and bound to five cis-acting elements enriched in the promoter regions of these transcription factors.
28246387	3	55	theme	Populus	493:499	arg1	PsnSHN2					507:513	PsnSHN2	507:513	PsnSHN2	507:513	Here, we identified a Populus gene, PsnSHN2, a counterpart of the Arabidopsis AP2/ERF type transcription factor, SHINE2.
28246387	3	55	theme	Populus	493:499	arg1	counterpart					518:528	a counterpart	516:528	a counterpart of the Arabidopsis AP2/ERF type transcription factor	516:581	Here, we identified a Populus gene, PsnSHN2, a counterpart of the Arabidopsis AP2/ERF type transcription factor, SHINE2.
28246387	3	55	theme	Populus	493:499	arg1	gene					501:504	a Populus gene	491:504	a Populus gene	491:504	Here, we identified a Populus gene, PsnSHN2, a counterpart of the Arabidopsis AP2/ERF type transcription factor, SHINE2.
28246387	2	56	attach	linked	283:288	arg2	number					241:246	a number	239:246	a number of transcription factors	239:271	Although a number of transcription factors have been linked to the regulation of wood formation process, none of them has been demonstrated to be a higher hierarchical regulator that coordinately regulates secondary wall biosynthesis genes.
28246387	2	56	attach	linked	283:288	arg1	regulation					297:306	the regulation	293:306	the regulation of wood formation process	293:332	Although a number of transcription factors have been linked to the regulation of wood formation process, none of them has been demonstrated to be a higher hierarchical regulator that coordinately regulates secondary wall biosynthesis genes.
28246387	8	57	theme	factors	1532:1538	arg1	up/down-regulation					1481:1498	selective up/down-regulation	1471:1498	selective up/down-regulation of its downstream transcription factors that control secondary wall formation	1471:1576	Taken together, our results suggest PsnSHN2 coordinately regulate secondary wall formation through selective up/down-regulation of its downstream transcription factors that control secondary wall formation.
28246387	8	58	theme	secondary	1553:1561	arg1	formation					1568:1576	secondary wall formation	1553:1576	secondary wall formation	1553:1576	Taken together, our results suggest PsnSHN2 coordinately regulate secondary wall formation through selective up/down-regulation of its downstream transcription factors that control secondary wall formation.
28246387	2	59	theme	factors	265:271	arg1	number					241:246	a number	239:246	a number of transcription factors	239:271	Although a number of transcription factors have been linked to the regulation of wood formation process, none of them has been demonstrated to be a higher hierarchical regulator that coordinately regulates secondary wall biosynthesis genes.
28246387	2	60	theme	process	326:332	arg1	regulation					297:306	the regulation	293:306	the regulation of wood formation process	293:332	Although a number of transcription factors have been linked to the regulation of wood formation process, none of them has been demonstrated to be a higher hierarchical regulator that coordinately regulates secondary wall biosynthesis genes.
28246387	0	61	theme	Secondary	76:84	arg1	Components					91:100	Secondary Wall Components	76:100	Secondary Wall Components	76:100	Functional Characterization of Populus PsnSHN2 in Coordinated Regulation of Secondary Wall Components in Tobacco.
28246387	6	62	theme	lignin	1109:1114	arg1	content					1098:1104	the content	1094:1104	the content of lignin that decreased 34%	1094:1133	The most significant changes occurred in the contents of cellulose and hemicellulose that increased 37% and 28%, respectively, whereas the content of lignin that decreased 34%.
28246387	2	63	theme	transcription	251:263	arg1	factors					265:271	transcription factors	251:271	transcription factors	251:271	Although a number of transcription factors have been linked to the regulation of wood formation process, none of them has been demonstrated to be a higher hierarchical regulator that coordinately regulates secondary wall biosynthesis genes.
28246387	1	64	from	biomass	200:206	arg1	earth					211:215	earth	211:215	earth	211:215	Wood formation is a biological process during which the most abundant lignocellulosic biomass on earth is produced.
28246387	4	65	theme	hierarchical	674:685	arg1	activator					703:711	a high hierarchical transcriptional activator	667:711	a high hierarchical transcriptional activator	667:711	PsnSHN2 is predominantly expressed in xylem tissues and acted evidently as a high hierarchical transcriptional activator.
28246387	7	66	theme	promoter	1323:1330	arg1	regions					1332:1338	the promoter regions	1319:1338	the promoter regions of these transcription factors	1319:1369	Furthermore, PsnSHN2 activated or repressed the promoter activities of transcription factors involved in secondary wall biosynthesis and bound to five cis-acting elements enriched in the promoter regions of these transcription factors.
28246387	7	67	theme	wall	1251:1254	arg1	biosynthesis					1256:1267	secondary wall biosynthesis	1241:1267	secondary wall biosynthesis	1241:1267	Furthermore, PsnSHN2 activated or repressed the promoter activities of transcription factors involved in secondary wall biosynthesis and bound to five cis-acting elements enriched in the promoter regions of these transcription factors.
28246387	5	68	theme	secondary	853:861	arg1	formation					868:876	secondary wall formation	853:876	secondary wall formation	853:876	Overexpression of PsnSHN2 in tobacco significantly altered the expression of both transcription factors and biosynthesis genes involved in secondary wall formation, leading to the thickened secondary walls and the changed cell wall composition.
28246387	4	69	theme	high	669:672	arg1	activator					703:711	a high hierarchical transcriptional activator	667:711	a high hierarchical transcriptional activator	667:711	PsnSHN2 is predominantly expressed in xylem tissues and acted evidently as a high hierarchical transcriptional activator.
28246387	7	70	theme	factors	1221:1227	arg1	activities					1193:1202	the promoter activities	1180:1202	the promoter activities of transcription factors involved in secondary wall biosynthesis	1180:1267	Furthermore, PsnSHN2 activated or repressed the promoter activities of transcription factors involved in secondary wall biosynthesis and bound to five cis-acting elements enriched in the promoter regions of these transcription factors.
25831127	0	0	theme	surface	109:115	arg1	methodology					117:127	response surface methodology	100:127	response surface methodology	100:127	Optimization of anticancer exopolysaccharide production from probiotic Lactobacillus acidophilus by response surface methodology.
25831127	5	1	theme	EPS	732:734	arg1	treatment					736:744	EPS treatment	732:744	EPS treatment	732:744	Quantitative polymerase chain reaction (qPCR) studies demonstrated that EPS treatment upregulated the expression of peroxisome proliferator activator receptor-γ (PPAR-γ) in both normoxia and hypoxia conditions, whereas it upregulated the expression of erythropoietin (EPO) in the normoxic condition, but there was no significant expression under hypoxic conditions.
25831127	7	2	theme	EPS	1167:1169	arg1	production					1153:1162	The optimized production	1139:1162	The optimized production of EPS at 24 hr	1139:1178	The optimized production of EPS at 24 hr was found to be 400 mg/L.
25831127	7	2	theme	EPS	1167:1169	arg1	mg/L					1200:1203	400 mg/L	1196:1203	400 mg/L	1196:1203	The optimized production of EPS at 24 hr was found to be 400 mg/L.
25831127	0	3	theme	response	100:107	arg1	methodology					117:127	response surface methodology	100:127	response surface methodology	100:127	Optimization of anticancer exopolysaccharide production from probiotic Lactobacillus acidophilus by response surface methodology.
25831127	1	4	theme	leading	168:174	arg1	causes					176:181	the leading causes	164:181	the leading causes of cancer-related deaths in the Western world	164:227	Colorectal cancer (CRC) is one of the leading causes of cancer-related deaths in the Western world.
25831127	4	5	theme	hypoxic	556:562	arg1	conditions					564:573	both normoxic and hypoxic conditions	538:573	both normoxic and hypoxic conditions	538:573	The experiment was performed in both normoxic and hypoxic conditions, and EPS was found to reduce the survival of CaCo2 cell line in both the conditions.
25831127	0	6	from	Optimization	0:11	arg1	acidophilus					85:95	probiotic Lactobacillus acidophilus	61:95	probiotic Lactobacillus acidophilus	61:95	Optimization of anticancer exopolysaccharide production from probiotic Lactobacillus acidophilus by response surface methodology.
25831127	4	7	theme	cell	626:629	arg1	line					631:634	CaCo2 cell line	620:634	CaCo2 cell line	620:634	The experiment was performed in both normoxic and hypoxic conditions, and EPS was found to reduce the survival of CaCo2 cell line in both the conditions.
25831127	8	8	theme	EPS	1279:1281	arg1	concentration					1283:1295	an EPS concentration	1276:1295	an EPS concentration of 597 mg/L	1276:1307	During batch cultivation the production peaked at 21 hr, resulting in an EPS concentration of 597 mg/L.
25831127	3	9	theme	present	334:340	arg1	study					342:346	the present study	330:346	the present study	330:346	In the present study, exopolysaccharide (EPS) extracted from Lactobacillus acidophilus was found to inhibit the growth of CaCo2 colon cancer cell line in a dose-dependent manner.
25831127	6	10	theme	central	1104:1110	arg1	design					1131:1136	central composite rotatory design	1104:1136	central composite rotatory design	1104:1136	Hence, the EPS production was optimized by Plackett-Burman design followed by central composite rotatory design.
25831127	8	11	theme	batch	1213:1217	arg1	cultivation					1219:1229	batch cultivation	1213:1229	batch cultivation	1213:1229	During batch cultivation the production peaked at 21 hr, resulting in an EPS concentration of 597 mg/L.
25831127	5	12	theme	normoxic	940:947	arg1	condition					949:957	the normoxic condition	936:957	the normoxic condition	936:957	Quantitative polymerase chain reaction (qPCR) studies demonstrated that EPS treatment upregulated the expression of peroxisome proliferator activator receptor-γ (PPAR-γ) in both normoxia and hypoxia conditions, whereas it upregulated the expression of erythropoietin (EPO) in the normoxic condition, but there was no significant expression under hypoxic conditions.
25831127	1	13	theme	causes	176:181	arg1	causes					176:181	the leading causes	164:181	the leading causes of cancer-related deaths in the Western world	164:227	Colorectal cancer (CRC) is one of the leading causes of cancer-related deaths in the Western world.
25831127	1	13	theme	causes	176:181	arg1	one					157:159	one	157:159	one	157:159	Colorectal cancer (CRC) is one of the leading causes of cancer-related deaths in the Western world.
25831127	6	14	theme	rotatory	1122:1129	arg1	design					1131:1136	central composite rotatory design	1104:1136	central composite rotatory design	1104:1136	Hence, the EPS production was optimized by Plackett-Burman design followed by central composite rotatory design.
25831127	5	15	from	expression	898:907	arg1	condition					949:957	the normoxic condition	936:957	the normoxic condition	936:957	Quantitative polymerase chain reaction (qPCR) studies demonstrated that EPS treatment upregulated the expression of peroxisome proliferator activator receptor-γ (PPAR-γ) in both normoxia and hypoxia conditions, whereas it upregulated the expression of erythropoietin (EPO) in the normoxic condition, but there was no significant expression under hypoxic conditions.
25831127	4	16	theme	CaCo2	620:624	arg1	line					631:634	CaCo2 cell line	620:634	CaCo2 cell line	620:634	The experiment was performed in both normoxic and hypoxic conditions, and EPS was found to reduce the survival of CaCo2 cell line in both the conditions.
25831127	5	17	theme	peroxisome	776:785	arg1	PPAR-γ					822:827	PPAR-γ	822:827	PPAR-γ	822:827	Quantitative polymerase chain reaction (qPCR) studies demonstrated that EPS treatment upregulated the expression of peroxisome proliferator activator receptor-γ (PPAR-γ) in both normoxia and hypoxia conditions, whereas it upregulated the expression of erythropoietin (EPO) in the normoxic condition, but there was no significant expression under hypoxic conditions.
25831127	5	17	theme	peroxisome	776:785	arg1	receptor-γ					810:819	peroxisome proliferator activator receptor-γ	776:819	peroxisome proliferator activator receptor-γ (PPAR-γ)	776:828	Quantitative polymerase chain reaction (qPCR) studies demonstrated that EPS treatment upregulated the expression of peroxisome proliferator activator receptor-γ (PPAR-γ) in both normoxia and hypoxia conditions, whereas it upregulated the expression of erythropoietin (EPO) in the normoxic condition, but there was no significant expression under hypoxic conditions.
25831127	5	18	theme	erythropoietin	912:925	arg1	expression					898:907	the expression	894:907	the expression of erythropoietin (EPO) in the normoxic condition	894:957	Quantitative polymerase chain reaction (qPCR) studies demonstrated that EPS treatment upregulated the expression of peroxisome proliferator activator receptor-γ (PPAR-γ) in both normoxia and hypoxia conditions, whereas it upregulated the expression of erythropoietin (EPO) in the normoxic condition, but there was no significant expression under hypoxic conditions.
25831127	1	19	theme	cancer-related	186:199	arg1	deaths					201:206	cancer-related deaths	186:206	cancer-related deaths in the Western world	186:227	Colorectal cancer (CRC) is one of the leading causes of cancer-related deaths in the Western world.
25831127	5	20	theme	proliferator	787:798	arg1	PPAR-γ					822:827	PPAR-γ	822:827	PPAR-γ	822:827	Quantitative polymerase chain reaction (qPCR) studies demonstrated that EPS treatment upregulated the expression of peroxisome proliferator activator receptor-γ (PPAR-γ) in both normoxia and hypoxia conditions, whereas it upregulated the expression of erythropoietin (EPO) in the normoxic condition, but there was no significant expression under hypoxic conditions.
25831127	5	20	theme	proliferator	787:798	arg1	receptor-γ					810:819	peroxisome proliferator activator receptor-γ	776:819	peroxisome proliferator activator receptor-γ (PPAR-γ)	776:828	Quantitative polymerase chain reaction (qPCR) studies demonstrated that EPS treatment upregulated the expression of peroxisome proliferator activator receptor-γ (PPAR-γ) in both normoxia and hypoxia conditions, whereas it upregulated the expression of erythropoietin (EPO) in the normoxic condition, but there was no significant expression under hypoxic conditions.
25831127	8	21	theme	mg/L	1304:1307	arg1	concentration					1283:1295	an EPS concentration	1276:1295	an EPS concentration of 597 mg/L	1276:1307	During batch cultivation the production peaked at 21 hr, resulting in an EPS concentration of 597 mg/L.
25831127	1	22	theme	deaths	201:206	arg1	causes					176:181	the leading causes	164:181	the leading causes of cancer-related deaths in the Western world	164:227	Colorectal cancer (CRC) is one of the leading causes of cancer-related deaths in the Western world.
25831127	0	23	theme	exopolysaccharide	27:43	arg1	production					45:54	anticancer exopolysaccharide production	16:54	anticancer exopolysaccharide production from probiotic Lactobacillus acidophilus	16:95	Optimization of anticancer exopolysaccharide production from probiotic Lactobacillus acidophilus by response surface methodology.
25831127	5	24	theme	Quantitative	660:671	arg1	qPCR					700:703	qPCR	700:703	qPCR	700:703	Quantitative polymerase chain reaction (qPCR) studies demonstrated that EPS treatment upregulated the expression of peroxisome proliferator activator receptor-γ (PPAR-γ) in both normoxia and hypoxia conditions, whereas it upregulated the expression of erythropoietin (EPO) in the normoxic condition, but there was no significant expression under hypoxic conditions.
25831127	5	24	theme	Quantitative	660:671	arg1	reaction					690:697	Quantitative polymerase chain reaction	660:697	Quantitative polymerase chain reaction (qPCR) studies	660:712	Quantitative polymerase chain reaction (qPCR) studies demonstrated that EPS treatment upregulated the expression of peroxisome proliferator activator receptor-γ (PPAR-γ) in both normoxia and hypoxia conditions, whereas it upregulated the expression of erythropoietin (EPO) in the normoxic condition, but there was no significant expression under hypoxic conditions.
25831127	3	25	located	found	418:422	arg2	exopolysaccharide					349:365	exopolysaccharide	349:365	exopolysaccharide (EPS) extracted from Lactobacillus acidophilus	349:412	In the present study, exopolysaccharide (EPS) extracted from Lactobacillus acidophilus was found to inhibit the growth of CaCo2 colon cancer cell line in a dose-dependent manner.
25831127	3	25	located	found	418:422	arg1	study					342:346	the present study	330:346	the present study	330:346	In the present study, exopolysaccharide (EPS) extracted from Lactobacillus acidophilus was found to inhibit the growth of CaCo2 colon cancer cell line in a dose-dependent manner.
25831127	3	25	located	found	418:422	arg2	EPS					368:370	EPS	368:370	EPS	368:370	In the present study, exopolysaccharide (EPS) extracted from Lactobacillus acidophilus was found to inhibit the growth of CaCo2 colon cancer cell line in a dose-dependent manner.
25831127	2	26	theme	CRC	298:300	arg1	risk					290:293	the risk	286:293	the risk of CRC	286:300	Recently, much attention has been focused on decreasing the risk of CRC by consuming probiotics.
25831127	4	27	from	survival	608:615	arg1	conditions					648:657	both the conditions	639:657	both the conditions	639:657	The experiment was performed in both normoxic and hypoxic conditions, and EPS was found to reduce the survival of CaCo2 cell line in both the conditions.
25831127	3	28	theme	CaCo2	449:453	arg1	line					473:476	CaCo2 colon cancer cell line	449:476	CaCo2 colon cancer cell line	449:476	In the present study, exopolysaccharide (EPS) extracted from Lactobacillus acidophilus was found to inhibit the growth of CaCo2 colon cancer cell line in a dose-dependent manner.
25831127	0	29	theme	anticancer	16:25	arg1	production					45:54	anticancer exopolysaccharide production	16:54	anticancer exopolysaccharide production from probiotic Lactobacillus acidophilus	16:95	Optimization of anticancer exopolysaccharide production from probiotic Lactobacillus acidophilus by response surface methodology.
25831127	5	30	theme	polymerase	673:682	arg1	qPCR					700:703	qPCR	700:703	qPCR	700:703	Quantitative polymerase chain reaction (qPCR) studies demonstrated that EPS treatment upregulated the expression of peroxisome proliferator activator receptor-γ (PPAR-γ) in both normoxia and hypoxia conditions, whereas it upregulated the expression of erythropoietin (EPO) in the normoxic condition, but there was no significant expression under hypoxic conditions.
25831127	5	30	theme	polymerase	673:682	arg1	reaction					690:697	Quantitative polymerase chain reaction	660:697	Quantitative polymerase chain reaction (qPCR) studies	660:712	Quantitative polymerase chain reaction (qPCR) studies demonstrated that EPS treatment upregulated the expression of peroxisome proliferator activator receptor-γ (PPAR-γ) in both normoxia and hypoxia conditions, whereas it upregulated the expression of erythropoietin (EPO) in the normoxic condition, but there was no significant expression under hypoxic conditions.
25831127	3	31	theme	dose-dependent	483:496	arg1	manner					498:503	a dose-dependent manner	481:503	a dose-dependent manner	481:503	In the present study, exopolysaccharide (EPS) extracted from Lactobacillus acidophilus was found to inhibit the growth of CaCo2 colon cancer cell line in a dose-dependent manner.
25831127	3	32	theme	colon	455:459	arg1	line					473:476	CaCo2 colon cancer cell line	449:476	CaCo2 colon cancer cell line	449:476	In the present study, exopolysaccharide (EPS) extracted from Lactobacillus acidophilus was found to inhibit the growth of CaCo2 colon cancer cell line in a dose-dependent manner.
25831127	4	33	theme	normoxic	543:550	arg1	conditions					564:573	both normoxic and hypoxic conditions	538:573	both normoxic and hypoxic conditions	538:573	The experiment was performed in both normoxic and hypoxic conditions, and EPS was found to reduce the survival of CaCo2 cell line in both the conditions.
25831127	5	34	theme	chain	684:688	arg1	qPCR					700:703	qPCR	700:703	qPCR	700:703	Quantitative polymerase chain reaction (qPCR) studies demonstrated that EPS treatment upregulated the expression of peroxisome proliferator activator receptor-γ (PPAR-γ) in both normoxia and hypoxia conditions, whereas it upregulated the expression of erythropoietin (EPO) in the normoxic condition, but there was no significant expression under hypoxic conditions.
25831127	5	34	theme	chain	684:688	arg1	reaction					690:697	Quantitative polymerase chain reaction	660:697	Quantitative polymerase chain reaction (qPCR) studies	660:712	Quantitative polymerase chain reaction (qPCR) studies demonstrated that EPS treatment upregulated the expression of peroxisome proliferator activator receptor-γ (PPAR-γ) in both normoxia and hypoxia conditions, whereas it upregulated the expression of erythropoietin (EPO) in the normoxic condition, but there was no significant expression under hypoxic conditions.
25831127	2	35	theme	much	240:243	arg1	attention					245:253	much attention	240:253	much attention	240:253	Recently, much attention has been focused on decreasing the risk of CRC by consuming probiotics.
25831127	4	36	theme	line	631:634	arg1	survival					608:615	the survival	604:615	the survival of CaCo2 cell line in both the conditions	604:657	The experiment was performed in both normoxic and hypoxic conditions, and EPS was found to reduce the survival of CaCo2 cell line in both the conditions.
25831127	1	37	theme	Western	215:221	arg1	world					223:227	the Western world	211:227	the Western world	211:227	Colorectal cancer (CRC) is one of the leading causes of cancer-related deaths in the Western world.
25831127	0	38	theme	production	45:54	arg1	Optimization					0:11	Optimization	0:11	Optimization of anticancer exopolysaccharide production from probiotic Lactobacillus acidophilus by response surface methodology.	0:128	Optimization of anticancer exopolysaccharide production from probiotic Lactobacillus acidophilus by response surface methodology.
25831127	5	39	theme	reaction	690:697	arg1	studies					706:712	Quantitative polymerase chain reaction (qPCR) studies	660:712	Quantitative polymerase chain reaction (qPCR) studies	660:712	Quantitative polymerase chain reaction (qPCR) studies demonstrated that EPS treatment upregulated the expression of peroxisome proliferator activator receptor-γ (PPAR-γ) in both normoxia and hypoxia conditions, whereas it upregulated the expression of erythropoietin (EPO) in the normoxic condition, but there was no significant expression under hypoxic conditions.
25831127	5	40	theme	activator	800:808	arg1	PPAR-γ					822:827	PPAR-γ	822:827	PPAR-γ	822:827	Quantitative polymerase chain reaction (qPCR) studies demonstrated that EPS treatment upregulated the expression of peroxisome proliferator activator receptor-γ (PPAR-γ) in both normoxia and hypoxia conditions, whereas it upregulated the expression of erythropoietin (EPO) in the normoxic condition, but there was no significant expression under hypoxic conditions.
25831127	5	40	theme	activator	800:808	arg1	receptor-γ					810:819	peroxisome proliferator activator receptor-γ	776:819	peroxisome proliferator activator receptor-γ (PPAR-γ)	776:828	Quantitative polymerase chain reaction (qPCR) studies demonstrated that EPS treatment upregulated the expression of peroxisome proliferator activator receptor-γ (PPAR-γ) in both normoxia and hypoxia conditions, whereas it upregulated the expression of erythropoietin (EPO) in the normoxic condition, but there was no significant expression under hypoxic conditions.
25831127	0	41	from	acidophilus	85:95	arg1	Optimization					0:11	Optimization	0:11	Optimization of anticancer exopolysaccharide production from probiotic Lactobacillus acidophilus by response surface methodology.	0:128	Optimization of anticancer exopolysaccharide production from probiotic Lactobacillus acidophilus by response surface methodology.
25831127	0	41	from	acidophilus	85:95	arg1	production					45:54	anticancer exopolysaccharide production	16:54	anticancer exopolysaccharide production from probiotic Lactobacillus acidophilus	16:95	Optimization of anticancer exopolysaccharide production from probiotic Lactobacillus acidophilus by response surface methodology.
25831127	1	42	from	world	223:227	arg1	causes					176:181	the leading causes	164:181	the leading causes of cancer-related deaths in the Western world	164:227	Colorectal cancer (CRC) is one of the leading causes of cancer-related deaths in the Western world.
25831127	3	43	theme	line	473:476	arg1	growth					439:444	the growth	435:444	the growth of CaCo2 colon cancer cell line	435:476	In the present study, exopolysaccharide (EPS) extracted from Lactobacillus acidophilus was found to inhibit the growth of CaCo2 colon cancer cell line in a dose-dependent manner.
25831127	5	44	theme	hypoxic	1006:1012	arg1	conditions					1014:1023	hypoxic conditions	1006:1023	hypoxic conditions	1006:1023	Quantitative polymerase chain reaction (qPCR) studies demonstrated that EPS treatment upregulated the expression of peroxisome proliferator activator receptor-γ (PPAR-γ) in both normoxia and hypoxia conditions, whereas it upregulated the expression of erythropoietin (EPO) in the normoxic condition, but there was no significant expression under hypoxic conditions.
25831127	5	45	dep	normoxia	838:845	arg1	conditions					859:868	conditions	859:868	conditions	859:868	Quantitative polymerase chain reaction (qPCR) studies demonstrated that EPS treatment upregulated the expression of peroxisome proliferator activator receptor-γ (PPAR-γ) in both normoxia and hypoxia conditions, whereas it upregulated the expression of erythropoietin (EPO) in the normoxic condition, but there was no significant expression under hypoxic conditions.
25831127	5	46	theme	significant	977:987	arg1	expression					989:998	no significant expression	974:998	no significant expression	974:998	Quantitative polymerase chain reaction (qPCR) studies demonstrated that EPS treatment upregulated the expression of peroxisome proliferator activator receptor-γ (PPAR-γ) in both normoxia and hypoxia conditions, whereas it upregulated the expression of erythropoietin (EPO) in the normoxic condition, but there was no significant expression under hypoxic conditions.
25831127	3	47	theme	cancer	461:466	arg1	line					473:476	CaCo2 colon cancer cell line	449:476	CaCo2 colon cancer cell line	449:476	In the present study, exopolysaccharide (EPS) extracted from Lactobacillus acidophilus was found to inhibit the growth of CaCo2 colon cancer cell line in a dose-dependent manner.
25831127	1	48	theme	Colorectal	130:139	arg1	CRC					149:151	CRC	149:151	CRC	149:151	Colorectal cancer (CRC) is one of the leading causes of cancer-related deaths in the Western world.
25831127	1	48	theme	Colorectal	130:139	arg1	cancer					141:146	Colorectal cancer	130:146	Colorectal cancer (CRC)	130:152	Colorectal cancer (CRC) is one of the leading causes of cancer-related deaths in the Western world.
25831127	6	49	theme	composite	1112:1120	arg1	design					1131:1136	central composite rotatory design	1104:1136	central composite rotatory design	1104:1136	Hence, the EPS production was optimized by Plackett-Burman design followed by central composite rotatory design.
25831127	7	50	from	hr	1177:1178	arg1	production					1153:1162	The optimized production	1139:1162	The optimized production of EPS at 24 hr	1139:1178	The optimized production of EPS at 24 hr was found to be 400 mg/L.
25831127	7	50	from	hr	1177:1178	arg1	mg/L					1200:1203	400 mg/L	1196:1203	400 mg/L	1196:1203	The optimized production of EPS at 24 hr was found to be 400 mg/L.
25831127	3	51	theme	cell	468:471	arg1	line					473:476	CaCo2 colon cancer cell line	449:476	CaCo2 colon cancer cell line	449:476	In the present study, exopolysaccharide (EPS) extracted from Lactobacillus acidophilus was found to inhibit the growth of CaCo2 colon cancer cell line in a dose-dependent manner.
25831127	1	52	from	deaths	201:206	arg1	world					223:227	the Western world	211:227	the Western world	211:227	Colorectal cancer (CRC) is one of the leading causes of cancer-related deaths in the Western world.
25831127	6	53	theme	Plackett-Burman	1069:1083	arg1	design					1085:1090	Plackett-Burman design	1069:1090	Plackett-Burman design followed by central composite rotatory design	1069:1136	Hence, the EPS production was optimized by Plackett-Burman design followed by central composite rotatory design.
25831127	6	54	theme	EPS	1037:1039	arg1	production					1041:1050	the EPS production	1033:1050	the EPS production	1033:1050	Hence, the EPS production was optimized by Plackett-Burman design followed by central composite rotatory design.
25831127	5	55	from	expression	762:771	arg1	normoxia					838:845	normoxia	838:845	normoxia	838:845	Quantitative polymerase chain reaction (qPCR) studies demonstrated that EPS treatment upregulated the expression of peroxisome proliferator activator receptor-γ (PPAR-γ) in both normoxia and hypoxia conditions, whereas it upregulated the expression of erythropoietin (EPO) in the normoxic condition, but there was no significant expression under hypoxic conditions.
25831127	5	55	from	expression	762:771	arg1	hypoxia					851:857	hypoxia	851:857	hypoxia	851:857	Quantitative polymerase chain reaction (qPCR) studies demonstrated that EPS treatment upregulated the expression of peroxisome proliferator activator receptor-γ (PPAR-γ) in both normoxia and hypoxia conditions, whereas it upregulated the expression of erythropoietin (EPO) in the normoxic condition, but there was no significant expression under hypoxic conditions.
25831127	5	56	theme	receptor-γ	810:819	arg1	expression					762:771	the expression	758:771	the expression of peroxisome proliferator activator receptor-γ (PPAR-γ) in both normoxia and hypoxia conditions	758:868	Quantitative polymerase chain reaction (qPCR) studies demonstrated that EPS treatment upregulated the expression of peroxisome proliferator activator receptor-γ (PPAR-γ) in both normoxia and hypoxia conditions, whereas it upregulated the expression of erythropoietin (EPO) in the normoxic condition, but there was no significant expression under hypoxic conditions.
25831127	1	57	from	causes	176:181	arg1	world					223:227	the Western world	211:227	the Western world	211:227	Colorectal cancer (CRC) is one of the leading causes of cancer-related deaths in the Western world.
25831127	7	58	theme	optimized	1143:1151	arg1	production					1153:1162	The optimized production	1139:1162	The optimized production of EPS at 24 hr	1139:1178	The optimized production of EPS at 24 hr was found to be 400 mg/L.
25831127	7	58	theme	optimized	1143:1151	arg1	mg/L					1200:1203	400 mg/L	1196:1203	400 mg/L	1196:1203	The optimized production of EPS at 24 hr was found to be 400 mg/L.
25498660	0	0	theme	carrageenan	91:101	arg1	films					117:121	carrageenan nanocomposite films	91:121	carrageenan nanocomposite films	91:121	Preparation, characterization, and antimicrobial activity of chitin nanofibrils reinforced carrageenan nanocomposite films.
25498660	5	1	theme	crystallite	548:558	arg1	4.73					587:590	4.73	587:590	4.73	587:590	The crystallite size of chitin and CNF was 4.73 and 6.27 nm, respectively.
25498660	5	1	theme	crystallite	548:558	arg1	size					560:563	The crystallite size	544:563	The crystallite size of chitin and CNF	544:581	The crystallite size of chitin and CNF was 4.73 and 6.27 nm, respectively.
25498660	1	2	theme	nanofibrils	176:186	arg1	preparation					154:164	the preparation	150:164	the preparation of chitin nanofibrils (CNF) reinforced carrageenan nanocomposite films by the solution-casting technique	150:269	Present study illustrates the preparation of chitin nanofibrils (CNF) reinforced carrageenan nanocomposite films by the solution-casting technique.
25498660	6	3	theme	char	623:626	arg1	content					628:634	The char content	619:634	The char content at 600 °C of chitin (19.2%)	619:662	The char content at 600 °C of chitin (19.2%) was lower than the CNF (25%).
25498660	6	3	theme	char	623:626	arg1	lower					668:672	lower	668:672	lower	668:672	The char content at 600 °C of chitin (19.2%) was lower than the CNF (25%).
25498660	8	4	theme	wt	954:955	arg1	%					956:956	up to 5 wt%	946:956	up to 5 wt%	946:956	The tensile strength and modulus of carrageenan film were increased significantly (p<0.05) after CNF reinforcement with up to 5 wt%, however, elongation at break, water vapor permeability, and transparency decreased slightly.
25498660	7	5	theme	polymer	810:816	arg1	matrix					818:823	the carrageenan polymer matrix	794:823	the carrageenan polymer matrix	794:823	The carrageenan/CNF composite films were smooth and flexible and the CNF was dispersed uniformly in the carrageenan polymer matrix.
25498660	8	6	from	transparency	1019:1030	arg1	elongation					968:977	elongation	968:977	elongation at break, water vapor permeability, and transparency	968:1030	The tensile strength and modulus of carrageenan film were increased significantly (p<0.05) after CNF reinforcement with up to 5 wt%, however, elongation at break, water vapor permeability, and transparency decreased slightly.
25498660	9	7	theme	antibacterial	1102:1114	arg1	activity					1116:1123	strong antibacterial activity	1095:1123	strong antibacterial activity	1095:1123	Carrageenan/CNF nanocomposite films showed strong antibacterial activity against a Gram-positive food-borne pathogen, Listeria monocytogenes.
25498660	0	8	theme	nanocomposite	103:115	arg1	films					117:121	carrageenan nanocomposite films	91:121	carrageenan nanocomposite films	91:121	Preparation, characterization, and antimicrobial activity of chitin nanofibrils reinforced carrageenan nanocomposite films.
25498660	4	9	theme	CNF	504:506	arg1	higher					524:529	higher	524:529	higher	524:529	However, the crystalinity of CNF was found to be higher than chitin.
25498660	4	9	theme	CNF	504:506	arg1	crystalinity					488:499	the crystalinity	484:499	the crystalinity of CNF	484:506	However, the crystalinity of CNF was found to be higher than chitin.
25498660	2	10	theme	chitin	311:316	arg1	hydrolysis					297:306	acid hydrolysis	292:306	acid hydrolysis of chitin	292:316	CNF was prepared by acid hydrolysis of chitin, followed by high speed homogenization and sonication.
25498660	8	11	dep	5	952:952	arg1	to					949:950	to	949:950	to	949:950	The tensile strength and modulus of carrageenan film were increased significantly (p<0.05) after CNF reinforcement with up to 5 wt%, however, elongation at break, water vapor permeability, and transparency decreased slightly.
25498660	3	12	theme	FTIR	373:376	arg1	result					378:383	FTIR result	373:383	FTIR result	373:383	FTIR result demonstrated that the chemical structure of chitin had not changed after acid hydrolysis.
25498660	8	13	theme	CNF	923:925	arg1	reinforcement					927:939	CNF reinforcement	923:939	CNF reinforcement	923:939	The tensile strength and modulus of carrageenan film were increased significantly (p<0.05) after CNF reinforcement with up to 5 wt%, however, elongation at break, water vapor permeability, and transparency decreased slightly.
25498660	7	14	theme	carrageenan	798:808	arg1	matrix					818:823	the carrageenan polymer matrix	794:823	the carrageenan polymer matrix	794:823	The carrageenan/CNF composite films were smooth and flexible and the CNF was dispersed uniformly in the carrageenan polymer matrix.
25498660	2	15	dep	prepared	280:287	arg1	followed					319:326	followed	319:326	followed by high speed homogenization and sonication	319:370	CNF was prepared by acid hydrolysis of chitin, followed by high speed homogenization and sonication.
25498660	5	16	theme	CNF	579:581	arg1	4.73					587:590	4.73	587:590	4.73	587:590	The crystallite size of chitin and CNF was 4.73 and 6.27 nm, respectively.
25498660	5	16	theme	CNF	579:581	arg1	size					560:563	The crystallite size	544:563	The crystallite size of chitin and CNF	544:581	The crystallite size of chitin and CNF was 4.73 and 6.27 nm, respectively.
25498660	2	17	theme	speed	336:340	arg1	homogenization					342:355	high speed homogenization	331:355	high speed homogenization	331:355	CNF was prepared by acid hydrolysis of chitin, followed by high speed homogenization and sonication.
25498660	2	18	theme	high	331:334	arg1	homogenization					342:355	high speed homogenization	331:355	high speed homogenization	331:355	CNF was prepared by acid hydrolysis of chitin, followed by high speed homogenization and sonication.
25498660	6	19	theme	chitin	649:654	arg1	°C					643:644	600 °C	639:644	600 °C of chitin (19.2%)	639:662	The char content at 600 °C of chitin (19.2%) was lower than the CNF (25%).
25498660	3	20	theme	acid	458:461	arg1	hydrolysis					463:472	acid hydrolysis	458:472	acid hydrolysis	458:472	FTIR result demonstrated that the chemical structure of chitin had not changed after acid hydrolysis.
25498660	8	21	theme	water	989:993	arg1	permeability					1001:1012	water vapor permeability	989:1012	water vapor permeability	989:1012	The tensile strength and modulus of carrageenan film were increased significantly (p<0.05) after CNF reinforcement with up to 5 wt%, however, elongation at break, water vapor permeability, and transparency decreased slightly.
25498660	6	22	dep	lower	668:672	arg1	%					690:690	25%	688:690	25%	688:690	The char content at 600 °C of chitin (19.2%) was lower than the CNF (25%).
25498660	8	23	theme	tensile	830:836	arg1	strength					838:845	The tensile strength	826:845	The tensile strength	826:845	The tensile strength and modulus of carrageenan film were increased significantly (p<0.05) after CNF reinforcement with up to 5 wt%, however, elongation at break, water vapor permeability, and transparency decreased slightly.
25498660	1	24	theme	nanocomposite	217:229	arg1	films					231:235	carrageenan nanocomposite films	205:235	carrageenan nanocomposite films	205:235	Present study illustrates the preparation of chitin nanofibrils (CNF) reinforced carrageenan nanocomposite films by the solution-casting technique.
25498660	3	25	theme	chemical	407:414	arg1	structure					416:424	the chemical structure	403:424	the chemical structure of chitin	403:434	FTIR result demonstrated that the chemical structure of chitin had not changed after acid hydrolysis.
25498660	9	26	theme	Carrageenan/CNF	1052:1066	arg1	films					1082:1086	Carrageenan/CNF nanocomposite films	1052:1086	Carrageenan/CNF nanocomposite films	1052:1086	Carrageenan/CNF nanocomposite films showed strong antibacterial activity against a Gram-positive food-borne pathogen, Listeria monocytogenes.
25498660	1	27	theme	Present	124:130	arg1	study					132:136	Present study	124:136	Present study	124:136	Present study illustrates the preparation of chitin nanofibrils (CNF) reinforced carrageenan nanocomposite films by the solution-casting technique.
25498660	0	28	theme	antimicrobial	35:47	arg1	activity					49:56	antimicrobial activity	35:56	antimicrobial activity	35:56	Preparation, characterization, and antimicrobial activity of chitin nanofibrils reinforced carrageenan nanocomposite films.
25498660	1	29	theme	carrageenan	205:215	arg1	films					231:235	carrageenan nanocomposite films	205:235	carrageenan nanocomposite films	205:235	Present study illustrates the preparation of chitin nanofibrils (CNF) reinforced carrageenan nanocomposite films by the solution-casting technique.
25498660	8	30	from	permeability	1001:1012	arg1	elongation					968:977	elongation	968:977	elongation at break, water vapor permeability, and transparency	968:1030	The tensile strength and modulus of carrageenan film were increased significantly (p<0.05) after CNF reinforcement with up to 5 wt%, however, elongation at break, water vapor permeability, and transparency decreased slightly.
25498660	8	31	from	break	982:986	arg1	elongation					968:977	elongation	968:977	elongation at break, water vapor permeability, and transparency	968:1030	The tensile strength and modulus of carrageenan film were increased significantly (p<0.05) after CNF reinforcement with up to 5 wt%, however, elongation at break, water vapor permeability, and transparency decreased slightly.
25498660	8	32	theme	vapor	995:999	arg1	permeability					1001:1012	water vapor permeability	989:1012	water vapor permeability	989:1012	The tensile strength and modulus of carrageenan film were increased significantly (p<0.05) after CNF reinforcement with up to 5 wt%, however, elongation at break, water vapor permeability, and transparency decreased slightly.
25498660	8	33	theme	carrageenan	862:872	arg1	film					874:877	carrageenan film	862:877	carrageenan film	862:877	The tensile strength and modulus of carrageenan film were increased significantly (p<0.05) after CNF reinforcement with up to 5 wt%, however, elongation at break, water vapor permeability, and transparency decreased slightly.
25498660	3	34	theme	chitin	429:434	arg1	structure					416:424	the chemical structure	403:424	the chemical structure of chitin	403:434	FTIR result demonstrated that the chemical structure of chitin had not changed after acid hydrolysis.
25498660	9	35	theme	Listeria	1170:1177	arg1	pathogen					1160:1167	a Gram-positive food-borne pathogen	1133:1167	a Gram-positive food-borne pathogen	1133:1167	Carrageenan/CNF nanocomposite films showed strong antibacterial activity against a Gram-positive food-borne pathogen, Listeria monocytogenes.
25498660	9	35	theme	Listeria	1170:1177	arg1	monocytogenes					1179:1191	Listeria monocytogenes	1170:1191	Listeria monocytogenes	1170:1191	Carrageenan/CNF nanocomposite films showed strong antibacterial activity against a Gram-positive food-borne pathogen, Listeria monocytogenes.
25498660	7	36	theme	carrageenan/CNF	698:712	arg1	films					724:728	The carrageenan/CNF composite films	694:728	The carrageenan/CNF composite films	694:728	The carrageenan/CNF composite films were smooth and flexible and the CNF was dispersed uniformly in the carrageenan polymer matrix.
25498660	7	36	theme	carrageenan/CNF	698:712	arg1	smooth					735:740	smooth	735:740	smooth	735:740	The carrageenan/CNF composite films were smooth and flexible and the CNF was dispersed uniformly in the carrageenan polymer matrix.
25498660	6	37	from	°C	643:644	arg1	content					628:634	The char content	619:634	The char content at 600 °C of chitin (19.2%)	619:662	The char content at 600 °C of chitin (19.2%) was lower than the CNF (25%).
25498660	6	37	from	°C	643:644	arg1	lower					668:672	lower	668:672	lower	668:672	The char content at 600 °C of chitin (19.2%) was lower than the CNF (25%).
25498660	5	38	theme	chitin	568:573	arg1	4.73					587:590	4.73	587:590	4.73	587:590	The crystallite size of chitin and CNF was 4.73 and 6.27 nm, respectively.
25498660	5	38	theme	chitin	568:573	arg1	size					560:563	The crystallite size	544:563	The crystallite size of chitin and CNF	544:581	The crystallite size of chitin and CNF was 4.73 and 6.27 nm, respectively.
25498660	7	39	theme	composite	714:722	arg1	films					724:728	The carrageenan/CNF composite films	694:728	The carrageenan/CNF composite films	694:728	The carrageenan/CNF composite films were smooth and flexible and the CNF was dispersed uniformly in the carrageenan polymer matrix.
25498660	7	39	theme	composite	714:722	arg1	smooth					735:740	smooth	735:740	smooth	735:740	The carrageenan/CNF composite films were smooth and flexible and the CNF was dispersed uniformly in the carrageenan polymer matrix.
25498660	9	40	theme	food-borne	1149:1158	arg1	pathogen					1160:1167	a Gram-positive food-borne pathogen	1133:1167	a Gram-positive food-borne pathogen	1133:1167	Carrageenan/CNF nanocomposite films showed strong antibacterial activity against a Gram-positive food-borne pathogen, Listeria monocytogenes.
25498660	9	40	theme	food-borne	1149:1158	arg1	monocytogenes					1179:1191	Listeria monocytogenes	1170:1191	Listeria monocytogenes	1170:1191	Carrageenan/CNF nanocomposite films showed strong antibacterial activity against a Gram-positive food-borne pathogen, Listeria monocytogenes.
25498660	2	41	theme	acid	292:295	arg1	hydrolysis					297:306	acid hydrolysis	292:306	acid hydrolysis of chitin	292:316	CNF was prepared by acid hydrolysis of chitin, followed by high speed homogenization and sonication.
25498660	8	42	theme	film	874:877	arg1	strength					838:845	The tensile strength	826:845	The tensile strength	826:845	The tensile strength and modulus of carrageenan film were increased significantly (p<0.05) after CNF reinforcement with up to 5 wt%, however, elongation at break, water vapor permeability, and transparency decreased slightly.
25498660	8	42	theme	film	874:877	arg1	modulus					851:857	modulus	851:857	modulus	851:857	The tensile strength and modulus of carrageenan film were increased significantly (p<0.05) after CNF reinforcement with up to 5 wt%, however, elongation at break, water vapor permeability, and transparency decreased slightly.
25498660	1	43	theme	solution-casting	244:259	arg1	technique					261:269	the solution-casting technique	240:269	the solution-casting technique	240:269	Present study illustrates the preparation of chitin nanofibrils (CNF) reinforced carrageenan nanocomposite films by the solution-casting technique.
25498660	9	44	theme	nanocomposite	1068:1080	arg1	films					1082:1086	Carrageenan/CNF nanocomposite films	1052:1086	Carrageenan/CNF nanocomposite films	1052:1086	Carrageenan/CNF nanocomposite films showed strong antibacterial activity against a Gram-positive food-borne pathogen, Listeria monocytogenes.
25498660	9	45	theme	Gram-positive	1135:1147	arg1	pathogen					1160:1167	a Gram-positive food-borne pathogen	1133:1167	a Gram-positive food-borne pathogen	1133:1167	Carrageenan/CNF nanocomposite films showed strong antibacterial activity against a Gram-positive food-borne pathogen, Listeria monocytogenes.
25498660	9	45	theme	Gram-positive	1135:1147	arg1	monocytogenes					1179:1191	Listeria monocytogenes	1170:1191	Listeria monocytogenes	1170:1191	Carrageenan/CNF nanocomposite films showed strong antibacterial activity against a Gram-positive food-borne pathogen, Listeria monocytogenes.
25498660	0	46	theme	nanofibrils	68:78	arg1	characterization					13:28	characterization	13:28	characterization	13:28	Preparation, characterization, and antimicrobial activity of chitin nanofibrils reinforced carrageenan nanocomposite films.
25498660	0	46	theme	nanofibrils	68:78	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation, characterization, and antimicrobial activity of chitin nanofibrils reinforced carrageenan nanocomposite films.
25498660	0	46	theme	nanofibrils	68:78	arg1	activity					49:56	antimicrobial activity	35:56	antimicrobial activity	35:56	Preparation, characterization, and antimicrobial activity of chitin nanofibrils reinforced carrageenan nanocomposite films.
25498660	8	47	dep	increased	884:892	arg1	p<0.05					909:914	p<0.05	909:914	p<0.05	909:914	The tensile strength and modulus of carrageenan film were increased significantly (p<0.05) after CNF reinforcement with up to 5 wt%, however, elongation at break, water vapor permeability, and transparency decreased slightly.
25498660	0	48	theme	chitin	61:66	arg1	nanofibrils					68:78	chitin nanofibrils	61:78	chitin nanofibrils	61:78	Preparation, characterization, and antimicrobial activity of chitin nanofibrils reinforced carrageenan nanocomposite films.
25498660	9	49	theme	strong	1095:1100	arg1	activity					1116:1123	strong antibacterial activity	1095:1123	strong antibacterial activity	1095:1123	Carrageenan/CNF nanocomposite films showed strong antibacterial activity against a Gram-positive food-borne pathogen, Listeria monocytogenes.
25498660	1	50	theme	chitin	169:174	arg1	CNF					189:191	CNF	189:191	CNF	189:191	Present study illustrates the preparation of chitin nanofibrils (CNF) reinforced carrageenan nanocomposite films by the solution-casting technique.
25498660	1	50	theme	chitin	169:174	arg1	nanofibrils					176:186	chitin nanofibrils	169:186	chitin nanofibrils (CNF) reinforced carrageenan nanocomposite films by the solution-casting technique	169:269	Present study illustrates the preparation of chitin nanofibrils (CNF) reinforced carrageenan nanocomposite films by the solution-casting technique.
28655919	5	0	theme	ChNF	552:555	arg1	scaffolds					557:565	ChNF scaffolds	552:565	ChNF scaffolds	552:565	Ag2O nanoparticles were evenly anchored on the surfaces of ChNF scaffolds via strong interactions with -NHCOCH3 groups, subsequently yielding Ag2O@ChNF heterostructured aerogels.
28655919	8	1	from	applications	1017:1028	arg1	disposal					1051:1058	radioactive waste disposal	1033:1058	radioactive waste disposal	1033:1058	The high adsorbability of the composite monolithic aerogel signifies its potential applications in radioactive waste disposal.
28655919	0	2	theme	Capture	93:99	arg1	Efficiency					101:110	High Iodine Capture Efficiency	81:110	High Iodine Capture Efficiency	81:110	Facile Fabrication of Nanofibrillated Chitin/Ag2O Heterostructured Aerogels with High Iodine Capture Efficiency.
28655919	4	3	theme	high	450:453	arg1	area					472:475	a high specific surface area	448:475	a high specific surface area of 179.71 m2/g	448:490	The ChNF aerogels exhibited a low bulk density of 2.19 mg/cm3 and a high specific surface area of 179.71 m2/g.
28655919	4	4	theme	bulk	416:419	arg1	density					421:427	a low bulk density	410:427	a low bulk density of 2.19 mg/cm3	410:442	The ChNF aerogels exhibited a low bulk density of 2.19 mg/cm3 and a high specific surface area of 179.71 m2/g.
28655919	7	5	theme	monoliths	882:890	arg1	capacity					856:863	The adsorption capacity	841:863	The adsorption capacity of the composite monoliths	841:890	The adsorption capacity of the composite monoliths can reach up to 2.81 mmol/g of I- anions.
28655919	5	6	with	interactions	578:589	arg1	groups					605:610	-NHCOCH3 groups	596:610	-NHCOCH3 groups	596:610	Ag2O nanoparticles were evenly anchored on the surfaces of ChNF scaffolds via strong interactions with -NHCOCH3 groups, subsequently yielding Ag2O@ChNF heterostructured aerogels.
28655919	5	7	theme	-NHCOCH3	596:603	arg1	groups					605:610	-NHCOCH3 groups	596:610	-NHCOCH3 groups	596:610	Ag2O nanoparticles were evenly anchored on the surfaces of ChNF scaffolds via strong interactions with -NHCOCH3 groups, subsequently yielding Ag2O@ChNF heterostructured aerogels.
28655919	4	8	theme	low	412:414	arg1	density					421:427	a low bulk density	410:427	a low bulk density of 2.19 mg/cm3	410:442	The ChNF aerogels exhibited a low bulk density of 2.19 mg/cm3 and a high specific surface area of 179.71 m2/g.
28655919	5	9	theme	Ag2O	635:638	arg1	aerogels					662:669	Ag2O@ChNF heterostructured aerogels	635:669	Ag2O@ChNF heterostructured aerogels	635:669	Ag2O nanoparticles were evenly anchored on the surfaces of ChNF scaffolds via strong interactions with -NHCOCH3 groups, subsequently yielding Ag2O@ChNF heterostructured aerogels.
28655919	6	10	theme	efficient	700:708	arg1	absorbents					710:719	efficient absorbents	700:719	efficient absorbents	700:719	The composites were used as efficient absorbents to remove radioiodine anions from water and capture a high amount of I2 vapor in the forms of AgI and iodine molecules.
28655919	6	10	theme	efficient	700:708	arg1	composites					676:685	The composites	672:685	The composites	672:685	The composites were used as efficient absorbents to remove radioiodine anions from water and capture a high amount of I2 vapor in the forms of AgI and iodine molecules.
28655919	8	11	theme	aerogel	985:991	arg1	adsorbability					943:955	The high adsorbability	934:955	The high adsorbability of the composite monolithic aerogel	934:991	The high adsorbability of the composite monolithic aerogel signifies its potential applications in radioactive waste disposal.
28655919	8	12	theme	potential	1007:1015	arg1	applications					1017:1028	its potential applications	1003:1028	its potential applications in radioactive waste disposal	1003:1058	The high adsorbability of the composite monolithic aerogel signifies its potential applications in radioactive waste disposal.
28655919	4	13	theme	179.71 m2/g	480:490	arg1	area					472:475	a high specific surface area	448:475	a high specific surface area of 179.71 m2/g	448:490	The ChNF aerogels exhibited a low bulk density of 2.19 mg/cm3 and a high specific surface area of 179.71 m2/g.
28655919	4	13	theme	179.71 m2/g	480:490	arg1	density					421:427	a low bulk density	410:427	a low bulk density of 2.19 mg/cm3	410:442	The ChNF aerogels exhibited a low bulk density of 2.19 mg/cm3 and a high specific surface area of 179.71 m2/g.
28655919	5	14	theme	ChNF	640:643	arg1	aerogels					662:669	Ag2O@ChNF heterostructured aerogels	635:669	Ag2O@ChNF heterostructured aerogels	635:669	Ag2O nanoparticles were evenly anchored on the surfaces of ChNF scaffolds via strong interactions with -NHCOCH3 groups, subsequently yielding Ag2O@ChNF heterostructured aerogels.
28655919	5	15	theme	@	639:639	arg1	aerogels					662:669	Ag2O@ChNF heterostructured aerogels	635:669	Ag2O@ChNF heterostructured aerogels	635:669	Ag2O nanoparticles were evenly anchored on the surfaces of ChNF scaffolds via strong interactions with -NHCOCH3 groups, subsequently yielding Ag2O@ChNF heterostructured aerogels.
28655919	2	16	from	groups	262:267	arg1	surface					287:293	the surface	283:293	the surface	283:293	Chitin was first fabricated into nanofibers with abundant acetyl amino groups (-NHCOCH3) on the surface.
28655919	5	17	theme	heterostructured	645:660	arg1	aerogels					662:669	Ag2O@ChNF heterostructured aerogels	635:669	Ag2O@ChNF heterostructured aerogels	635:669	Ag2O nanoparticles were evenly anchored on the surfaces of ChNF scaffolds via strong interactions with -NHCOCH3 groups, subsequently yielding Ag2O@ChNF heterostructured aerogels.
28655919	6	18	theme	radioiodine	731:741	arg1	anions					743:748	radioiodine anions	731:748	radioiodine anions	731:748	The composites were used as efficient absorbents to remove radioiodine anions from water and capture a high amount of I2 vapor in the forms of AgI and iodine molecules.
28655919	4	19	theme	ChNF	386:389	arg1	aerogels					391:398	The ChNF aerogels	382:398	The ChNF aerogels	382:398	The ChNF aerogels exhibited a low bulk density of 2.19 mg/cm3 and a high specific surface area of 179.71 m2/g.
28655919	0	20	theme	Facile	0:5	arg1	Fabrication					7:17	Facile Fabrication	0:17	Facile Fabrication of Nanofibrillated Chitin/Ag2O Heterostructured Aerogels with High Iodine Capture Efficiency	0:110	Facile Fabrication of Nanofibrillated Chitin/Ag2O Heterostructured Aerogels with High Iodine Capture Efficiency.
28655919	7	21	theme	anions	926:931	arg1	2.81 mmol/g					908:918	2.81 mmol/g	908:918	2.81 mmol/g of I- anions	908:931	The adsorption capacity of the composite monoliths can reach up to 2.81 mmol/g of I- anions.
28655919	1	22	theme	Nanofibrillated	113:127	arg1	aerogels					141:148	Nanofibrillated chitin/Ag2O aerogels	113:148	Nanofibrillated chitin/Ag2O aerogels	113:148	Nanofibrillated chitin/Ag2O aerogels were fabricated for radioiodine removal.
28655919	8	23	theme	waste	1045:1049	arg1	disposal					1051:1058	radioactive waste disposal	1033:1058	radioactive waste disposal	1033:1058	The high adsorbability of the composite monolithic aerogel signifies its potential applications in radioactive waste disposal.
28655919	8	24	theme	radioactive	1033:1043	arg1	disposal					1051:1058	radioactive waste disposal	1033:1058	radioactive waste disposal	1033:1058	The high adsorbability of the composite monolithic aerogel signifies its potential applications in radioactive waste disposal.
28655919	6	25	theme	vapor	793:797	arg1	amount					780:785	a high amount	773:785	a high amount of I2 vapor	773:797	The composites were used as efficient absorbents to remove radioiodine anions from water and capture a high amount of I2 vapor in the forms of AgI and iodine molecules.
28655919	6	25	theme	vapor	793:797	arg1	vapor					793:797	I2 vapor	790:797	I2 vapor	790:797	The composites were used as efficient absorbents to remove radioiodine anions from water and capture a high amount of I2 vapor in the forms of AgI and iodine molecules.
28655919	1	26	theme	chitin/Ag2O	129:139	arg1	aerogels					141:148	Nanofibrillated chitin/Ag2O aerogels	113:148	Nanofibrillated chitin/Ag2O aerogels	113:148	Nanofibrillated chitin/Ag2O aerogels were fabricated for radioiodine removal.
28655919	6	27	theme	iodine	823:828	arg1	molecules					830:838	iodine molecules	823:838	iodine molecules	823:838	The composites were used as efficient absorbents to remove radioiodine anions from water and capture a high amount of I2 vapor in the forms of AgI and iodine molecules.
28655919	3	28	theme	porous	309:314	arg1	nanofiber					323:331	highly porous chitin nanofiber	302:331	highly porous chitin nanofiber (ChNF) aerogels	302:347	Then, highly porous chitin nanofiber (ChNF) aerogels were obtained via freeze-drying.
28655919	3	28	theme	porous	309:314	arg1	ChNF					334:337	ChNF	334:337	ChNF	334:337	Then, highly porous chitin nanofiber (ChNF) aerogels were obtained via freeze-drying.
28655919	4	29	theme	surface	464:470	arg1	area					472:475	a high specific surface area	448:475	a high specific surface area of 179.71 m2/g	448:490	The ChNF aerogels exhibited a low bulk density of 2.19 mg/cm3 and a high specific surface area of 179.71 m2/g.
28655919	5	30	theme	Ag2O	493:496	arg1	nanoparticles					498:510	Ag2O nanoparticles	493:510	Ag2O nanoparticles	493:510	Ag2O nanoparticles were evenly anchored on the surfaces of ChNF scaffolds via strong interactions with -NHCOCH3 groups, subsequently yielding Ag2O@ChNF heterostructured aerogels.
28655919	7	31	theme	composite	872:880	arg1	monoliths					882:890	the composite monoliths	868:890	the composite monoliths	868:890	The adsorption capacity of the composite monoliths can reach up to 2.81 mmol/g of I- anions.
28655919	4	32	theme	specific	455:462	arg1	area					472:475	a high specific surface area	448:475	a high specific surface area of 179.71 m2/g	448:490	The ChNF aerogels exhibited a low bulk density of 2.19 mg/cm3 and a high specific surface area of 179.71 m2/g.
28655919	5	33	theme	scaffolds	557:565	arg1	surfaces					540:547	the surfaces	536:547	the surfaces of ChNF scaffolds	536:565	Ag2O nanoparticles were evenly anchored on the surfaces of ChNF scaffolds via strong interactions with -NHCOCH3 groups, subsequently yielding Ag2O@ChNF heterostructured aerogels.
28655919	7	34	theme	adsorption	845:854	arg1	capacity					856:863	The adsorption capacity	841:863	The adsorption capacity of the composite monoliths	841:890	The adsorption capacity of the composite monoliths can reach up to 2.81 mmol/g of I- anions.
28655919	6	35	theme	AgI	815:817	arg1	forms					806:810	the forms	802:810	the forms of AgI and iodine molecules	802:838	The composites were used as efficient absorbents to remove radioiodine anions from water and capture a high amount of I2 vapor in the forms of AgI and iodine molecules.
28655919	0	36	theme	Aerogels	67:74	arg1	Fabrication					7:17	Facile Fabrication	0:17	Facile Fabrication of Nanofibrillated Chitin/Ag2O Heterostructured Aerogels with High Iodine Capture Efficiency	0:110	Facile Fabrication of Nanofibrillated Chitin/Ag2O Heterostructured Aerogels with High Iodine Capture Efficiency.
28655919	2	37	theme	amino	256:260	arg1	-NHCOCH3					270:277	-NHCOCH3	270:277	-NHCOCH3	270:277	Chitin was first fabricated into nanofibers with abundant acetyl amino groups (-NHCOCH3) on the surface.
28655919	2	37	theme	amino	256:260	arg1	groups					262:267	abundant acetyl amino groups	240:267	abundant acetyl amino groups (-NHCOCH3) on the surface	240:293	Chitin was first fabricated into nanofibers with abundant acetyl amino groups (-NHCOCH3) on the surface.
28655919	4	38	theme	2.19 mg/cm3	432:442	arg1	area					472:475	a high specific surface area	448:475	a high specific surface area of 179.71 m2/g	448:490	The ChNF aerogels exhibited a low bulk density of 2.19 mg/cm3 and a high specific surface area of 179.71 m2/g.
28655919	4	38	theme	2.19 mg/cm3	432:442	arg1	density					421:427	a low bulk density	410:427	a low bulk density of 2.19 mg/cm3	410:442	The ChNF aerogels exhibited a low bulk density of 2.19 mg/cm3 and a high specific surface area of 179.71 m2/g.
28655919	0	39	with	Fabrication	7:17	arg1	Efficiency					101:110	High Iodine Capture Efficiency	81:110	High Iodine Capture Efficiency	81:110	Facile Fabrication of Nanofibrillated Chitin/Ag2O Heterostructured Aerogels with High Iodine Capture Efficiency.
28655919	3	40	theme	chitin	316:321	arg1	nanofiber					323:331	highly porous chitin nanofiber	302:331	highly porous chitin nanofiber (ChNF) aerogels	302:347	Then, highly porous chitin nanofiber (ChNF) aerogels were obtained via freeze-drying.
28655919	3	40	theme	chitin	316:321	arg1	ChNF					334:337	ChNF	334:337	ChNF	334:337	Then, highly porous chitin nanofiber (ChNF) aerogels were obtained via freeze-drying.
28655919	2	41	theme	acetyl	249:254	arg1	-NHCOCH3					270:277	-NHCOCH3	270:277	-NHCOCH3	270:277	Chitin was first fabricated into nanofibers with abundant acetyl amino groups (-NHCOCH3) on the surface.
28655919	2	41	theme	acetyl	249:254	arg1	groups					262:267	abundant acetyl amino groups	240:267	abundant acetyl amino groups (-NHCOCH3) on the surface	240:293	Chitin was first fabricated into nanofibers with abundant acetyl amino groups (-NHCOCH3) on the surface.
28655919	6	42	theme	high	775:778	arg1	amount					780:785	a high amount	773:785	a high amount of I2 vapor	773:797	The composites were used as efficient absorbents to remove radioiodine anions from water and capture a high amount of I2 vapor in the forms of AgI and iodine molecules.
28655919	6	42	theme	high	775:778	arg1	vapor					793:797	I2 vapor	790:797	I2 vapor	790:797	The composites were used as efficient absorbents to remove radioiodine anions from water and capture a high amount of I2 vapor in the forms of AgI and iodine molecules.
28655919	2	43	with	nanofibers	224:233	arg1	-NHCOCH3					270:277	-NHCOCH3	270:277	-NHCOCH3	270:277	Chitin was first fabricated into nanofibers with abundant acetyl amino groups (-NHCOCH3) on the surface.
28655919	2	43	with	nanofibers	224:233	arg1	groups					262:267	abundant acetyl amino groups	240:267	abundant acetyl amino groups (-NHCOCH3) on the surface	240:293	Chitin was first fabricated into nanofibers with abundant acetyl amino groups (-NHCOCH3) on the surface.
28655919	7	44	theme	I-	923:924	arg1	anions					926:931	I- anions	923:931	I- anions	923:931	The adsorption capacity of the composite monoliths can reach up to 2.81 mmol/g of I- anions.
28655919	5	45	theme	strong	571:576	arg1	interactions					578:589	strong interactions	571:589	strong interactions with -NHCOCH3 groups	571:610	Ag2O nanoparticles were evenly anchored on the surfaces of ChNF scaffolds via strong interactions with -NHCOCH3 groups, subsequently yielding Ag2O@ChNF heterostructured aerogels.
28655919	3	46	theme	nanofiber	323:331	arg1	aerogels					340:347	highly porous chitin nanofiber (ChNF) aerogels	302:347	highly porous chitin nanofiber (ChNF) aerogels	302:347	Then, highly porous chitin nanofiber (ChNF) aerogels were obtained via freeze-drying.
28655919	2	47	theme	abundant	240:247	arg1	-NHCOCH3					270:277	-NHCOCH3	270:277	-NHCOCH3	270:277	Chitin was first fabricated into nanofibers with abundant acetyl amino groups (-NHCOCH3) on the surface.
28655919	2	47	theme	abundant	240:247	arg1	groups					262:267	abundant acetyl amino groups	240:267	abundant acetyl amino groups (-NHCOCH3) on the surface	240:293	Chitin was first fabricated into nanofibers with abundant acetyl amino groups (-NHCOCH3) on the surface.
28655919	6	48	theme	I2	790:791	arg1	vapor					793:797	I2 vapor	790:797	I2 vapor	790:797	The composites were used as efficient absorbents to remove radioiodine anions from water and capture a high amount of I2 vapor in the forms of AgI and iodine molecules.
28655919	1	49	theme	radioiodine	170:180	arg1	removal					182:188	radioiodine removal	170:188	radioiodine removal	170:188	Nanofibrillated chitin/Ag2O aerogels were fabricated for radioiodine removal.
28655919	0	50	theme	Iodine	86:91	arg1	Efficiency					101:110	High Iodine Capture Efficiency	81:110	High Iodine Capture Efficiency	81:110	Facile Fabrication of Nanofibrillated Chitin/Ag2O Heterostructured Aerogels with High Iodine Capture Efficiency.
28655919	6	51	theme	molecules	830:838	arg1	forms					806:810	the forms	802:810	the forms of AgI and iodine molecules	802:838	The composites were used as efficient absorbents to remove radioiodine anions from water and capture a high amount of I2 vapor in the forms of AgI and iodine molecules.
28655919	8	52	theme	monolithic	974:983	arg1	aerogel					985:991	the composite monolithic aerogel	960:991	the composite monolithic aerogel	960:991	The high adsorbability of the composite monolithic aerogel signifies its potential applications in radioactive waste disposal.
28655919	0	53	theme	High	81:84	arg1	Efficiency					101:110	High Iodine Capture Efficiency	81:110	High Iodine Capture Efficiency	81:110	Facile Fabrication of Nanofibrillated Chitin/Ag2O Heterostructured Aerogels with High Iodine Capture Efficiency.
28655919	6	54	used	used	692:695	arg2	composites					676:685	The composites	672:685	The composites	672:685	The composites were used as efficient absorbents to remove radioiodine anions from water and capture a high amount of I2 vapor in the forms of AgI and iodine molecules.
28655919	6	54	used	used	692:695	arg2	absorbents					710:719	efficient absorbents	700:719	efficient absorbents	700:719	The composites were used as efficient absorbents to remove radioiodine anions from water and capture a high amount of I2 vapor in the forms of AgI and iodine molecules.
28655919	8	55	theme	composite	964:972	arg1	aerogel					985:991	the composite monolithic aerogel	960:991	the composite monolithic aerogel	960:991	The high adsorbability of the composite monolithic aerogel signifies its potential applications in radioactive waste disposal.
28655919	8	56	theme	high	938:941	arg1	adsorbability					943:955	The high adsorbability	934:955	The high adsorbability of the composite monolithic aerogel	934:991	The high adsorbability of the composite monolithic aerogel signifies its potential applications in radioactive waste disposal.
27148706	0	0	theme	polymeric	52:60	arg1	EPS					74:76	EPS	74:76	EPS	74:76	Effects of Fe(III) on biofilm and its extracellular polymeric substances (EPS) in fixed bed biofilm reactors.
27148706	0	0	theme	polymeric	52:60	arg1	substances					62:71	its extracellular polymeric substances	34:71	its extracellular polymeric substances (EPS)	34:77	Effects of Fe(III) on biofilm and its extracellular polymeric substances (EPS) in fixed bed biofilm reactors.
27148706	1	1	theme	extracellular	263:275	arg1	EPS					299:301	EPS	299:301	EPS	299:301	The effects of Fe(III) on the biofilm mass and activity, the biofilm micromorphology as well as the composition and functional groups characteristics of extracellular polymeric substances (EPS) in biofilm were investigated in laboratory-scale fixed bed biofilm reactors.
27148706	1	1	theme	extracellular	263:275	arg1	substances					287:296	extracellular polymeric substances	263:296	extracellular polymeric substances (EPS) in biofilm	263:313	The effects of Fe(III) on the biofilm mass and activity, the biofilm micromorphology as well as the composition and functional groups characteristics of extracellular polymeric substances (EPS) in biofilm were investigated in laboratory-scale fixed bed biofilm reactors.
27148706	1	2	from	groups	237:242	arg1	biofilm					307:313	biofilm	307:313	biofilm	307:313	The effects of Fe(III) on the biofilm mass and activity, the biofilm micromorphology as well as the composition and functional groups characteristics of extracellular polymeric substances (EPS) in biofilm were investigated in laboratory-scale fixed bed biofilm reactors.
27148706	4	3	theme	unfavourable	951:962	arg1	production					940:949	tightly bound EPS production	922:949	tightly bound EPS production unfavourable for substrate mass transfer	922:990	The result of the EPS composition suggested 2 mg/L of Fe(III) increased soluble EPS and loosely bound EPS which contributed to the microbial aggregation, while 16 mg/L of Fe(III) promoted tightly bound EPS production unfavourable for substrate mass transfer.
27148706	3	4	theme	filaments	675:682	arg1	reduction					658:666	significant reduction	647:666	significant reduction of the filaments, great promotion of the EPS secretion in biofilm	647:731	Scanning electron microscopy (SEM) study indicated a high concentration (16 mg/L) of Fe(III) led to significant reduction of the filaments, great promotion of the EPS secretion in biofilm.
27148706	0	5	theme	extracellular	38:50	arg1	EPS					74:76	EPS	74:76	EPS	74:76	Effects of Fe(III) on biofilm and its extracellular polymeric substances (EPS) in fixed bed biofilm reactors.
27148706	0	5	theme	extracellular	38:50	arg1	substances					62:71	its extracellular polymeric substances	34:71	its extracellular polymeric substances (EPS)	34:77	Effects of Fe(III) on biofilm and its extracellular polymeric substances (EPS) in fixed bed biofilm reactors.
27148706	1	6	theme	polymeric	277:285	arg1	EPS					299:301	EPS	299:301	EPS	299:301	The effects of Fe(III) on the biofilm mass and activity, the biofilm micromorphology as well as the composition and functional groups characteristics of extracellular polymeric substances (EPS) in biofilm were investigated in laboratory-scale fixed bed biofilm reactors.
27148706	1	6	theme	polymeric	277:285	arg1	substances					287:296	extracellular polymeric substances	263:296	extracellular polymeric substances (EPS) in biofilm	263:313	The effects of Fe(III) on the biofilm mass and activity, the biofilm micromorphology as well as the composition and functional groups characteristics of extracellular polymeric substances (EPS) in biofilm were investigated in laboratory-scale fixed bed biofilm reactors.
27148706	3	7	theme	Scanning	547:554	arg1	SEM					577:579	SEM	577:579	SEM	577:579	Scanning electron microscopy (SEM) study indicated a high concentration (16 mg/L) of Fe(III) led to significant reduction of the filaments, great promotion of the EPS secretion in biofilm.
27148706	3	7	theme	Scanning	547:554	arg1	microscopy					565:574	Scanning electron microscopy	547:574	Scanning electron microscopy (SEM) study	547:586	Scanning electron microscopy (SEM) study indicated a high concentration (16 mg/L) of Fe(III) led to significant reduction of the filaments, great promotion of the EPS secretion in biofilm.
27148706	2	8	theme	Fe	415:416	arg1	mg/L					407:410	2 mg/L	405:410	2 mg/L of Fe(III)	405:421	The results showed that 2 mg/L of Fe(III) promoted the biofilm mass and improved the biofilm activity, but 16 mg/L of Fe(III) adversely affected biofilm development.
27148706	3	9	theme	EPS	708:710	arg1	secretion					712:720	the EPS secretion	704:720	the EPS secretion	704:720	Scanning electron microscopy (SEM) study indicated a high concentration (16 mg/L) of Fe(III) led to significant reduction of the filaments, great promotion of the EPS secretion in biofilm.
27148706	1	10	theme	biofilm	171:177	arg1	micromorphology					179:193	the biofilm micromorphology	167:193	the biofilm micromorphology	167:193	The effects of Fe(III) on the biofilm mass and activity, the biofilm micromorphology as well as the composition and functional groups characteristics of extracellular polymeric substances (EPS) in biofilm were investigated in laboratory-scale fixed bed biofilm reactors.
27148706	2	11	theme	biofilm	436:442	arg1	mass					444:447	the biofilm mass	432:447	the biofilm mass	432:447	The results showed that 2 mg/L of Fe(III) promoted the biofilm mass and improved the biofilm activity, but 16 mg/L of Fe(III) adversely affected biofilm development.
27148706	1	12	theme	substances	287:296	arg1	mass					148:151	biofilm mass	140:151	biofilm mass	140:151	The effects of Fe(III) on the biofilm mass and activity, the biofilm micromorphology as well as the composition and functional groups characteristics of extracellular polymeric substances (EPS) in biofilm were investigated in laboratory-scale fixed bed biofilm reactors.
27148706	1	12	theme	substances	287:296	arg1	groups					237:242	functional groups	226:242	functional groups	226:242	The effects of Fe(III) on the biofilm mass and activity, the biofilm micromorphology as well as the composition and functional groups characteristics of extracellular polymeric substances (EPS) in biofilm were investigated in laboratory-scale fixed bed biofilm reactors.
27148706	1	12	theme	substances	287:296	arg1	activity					157:164	activity	157:164	activity	157:164	The effects of Fe(III) on the biofilm mass and activity, the biofilm micromorphology as well as the composition and functional groups characteristics of extracellular polymeric substances (EPS) in biofilm were investigated in laboratory-scale fixed bed biofilm reactors.
27148706	1	12	theme	substances	287:296	arg1	EPS					299:301	EPS	299:301	EPS	299:301	The effects of Fe(III) on the biofilm mass and activity, the biofilm micromorphology as well as the composition and functional groups characteristics of extracellular polymeric substances (EPS) in biofilm were investigated in laboratory-scale fixed bed biofilm reactors.
27148706	1	12	theme	substances	287:296	arg1	composition					210:220	composition	210:220	composition	210:220	The effects of Fe(III) on the biofilm mass and activity, the biofilm micromorphology as well as the composition and functional groups characteristics of extracellular polymeric substances (EPS) in biofilm were investigated in laboratory-scale fixed bed biofilm reactors.
27148706	1	12	theme	substances	287:296	arg1	substances					287:296	extracellular polymeric substances	263:296	extracellular polymeric substances (EPS) in biofilm	263:313	The effects of Fe(III) on the biofilm mass and activity, the biofilm micromorphology as well as the composition and functional groups characteristics of extracellular polymeric substances (EPS) in biofilm were investigated in laboratory-scale fixed bed biofilm reactors.
27148706	1	12	theme	substances	287:296	arg1	micromorphology					179:193	the biofilm micromorphology	167:193	the biofilm micromorphology	167:193	The effects of Fe(III) on the biofilm mass and activity, the biofilm micromorphology as well as the composition and functional groups characteristics of extracellular polymeric substances (EPS) in biofilm were investigated in laboratory-scale fixed bed biofilm reactors.
27148706	5	13	dep	Fourier	993:999	arg1	transform					1001:1009	transform	1001:1009	transform infrared spectroscopy and X-ray photoelectron spectroscopy analysis	1001:1077	Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy analysis demonstrated that Fe(III) exerted a significant influence on the -CONH- groups of proteins and the C-O groups of polysaccharides in EPS.
27148706	1	14	from	effects	114:120	arg1	activity					157:164	activity	157:164	activity	157:164	The effects of Fe(III) on the biofilm mass and activity, the biofilm micromorphology as well as the composition and functional groups characteristics of extracellular polymeric substances (EPS) in biofilm were investigated in laboratory-scale fixed bed biofilm reactors.
27148706	1	14	from	effects	114:120	arg1	mass					148:151	biofilm mass	140:151	biofilm mass	140:151	The effects of Fe(III) on the biofilm mass and activity, the biofilm micromorphology as well as the composition and functional groups characteristics of extracellular polymeric substances (EPS) in biofilm were investigated in laboratory-scale fixed bed biofilm reactors.
27148706	1	14	from	effects	114:120	arg1	composition					210:220	composition	210:220	composition	210:220	The effects of Fe(III) on the biofilm mass and activity, the biofilm micromorphology as well as the composition and functional groups characteristics of extracellular polymeric substances (EPS) in biofilm were investigated in laboratory-scale fixed bed biofilm reactors.
27148706	1	14	from	effects	114:120	arg1	micromorphology					179:193	the biofilm micromorphology	167:193	the biofilm micromorphology	167:193	The effects of Fe(III) on the biofilm mass and activity, the biofilm micromorphology as well as the composition and functional groups characteristics of extracellular polymeric substances (EPS) in biofilm were investigated in laboratory-scale fixed bed biofilm reactors.
27148706	3	15	theme	secretion	712:720	arg1	filaments					675:682	the filaments	671:682	the filaments	671:682	Scanning electron microscopy (SEM) study indicated a high concentration (16 mg/L) of Fe(III) led to significant reduction of the filaments, great promotion of the EPS secretion in biofilm.
27148706	3	15	theme	secretion	712:720	arg1	promotion					691:699	great promotion	685:699	great promotion of the EPS secretion in biofilm	685:731	Scanning electron microscopy (SEM) study indicated a high concentration (16 mg/L) of Fe(III) led to significant reduction of the filaments, great promotion of the EPS secretion in biofilm.
27148706	5	16	theme	infrared	1011:1018	arg1	spectroscopy					1020:1031	infrared spectroscopy	1011:1031	infrared spectroscopy	1011:1031	Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy analysis demonstrated that Fe(III) exerted a significant influence on the -CONH- groups of proteins and the C-O groups of polysaccharides in EPS.
27148706	6	17	theme	wastewater	1467:1476	arg1	treatment					1478:1486	iron ion containing wastewater treatment	1447:1486	iron ion containing wastewater treatment	1447:1486	This study reveals that Fe(III) influences biofilm development and activity not only by directly impacting the microbial physiology but by indirectly affecting the EPS constituents, and it helps to provide theoretical guidance for iron ion containing wastewater treatment.
27148706	0	18	from	biofilm	22:28	arg1	reactors					100:107	fixed bed biofilm reactors	82:107	fixed bed biofilm reactors	82:107	Effects of Fe(III) on biofilm and its extracellular polymeric substances (EPS) in fixed bed biofilm reactors.
27148706	6	19	theme	microbial	1327:1335	arg1	physiology					1337:1346	the microbial physiology	1323:1346	the microbial physiology	1323:1346	This study reveals that Fe(III) influences biofilm development and activity not only by directly impacting the microbial physiology but by indirectly affecting the EPS constituents, and it helps to provide theoretical guidance for iron ion containing wastewater treatment.
27148706	2	20	theme	biofilm	526:532	arg1	development					534:544	biofilm development	526:544	biofilm development	526:544	The results showed that 2 mg/L of Fe(III) promoted the biofilm mass and improved the biofilm activity, but 16 mg/L of Fe(III) adversely affected biofilm development.
27148706	4	21	theme	soluble	806:812	arg1	EPS					814:816	soluble EPS	806:816	soluble EPS	806:816	The result of the EPS composition suggested 2 mg/L of Fe(III) increased soluble EPS and loosely bound EPS which contributed to the microbial aggregation, while 16 mg/L of Fe(III) promoted tightly bound EPS production unfavourable for substrate mass transfer.
27148706	1	22	from	biofilm	307:313	arg1	mass					148:151	biofilm mass	140:151	biofilm mass	140:151	The effects of Fe(III) on the biofilm mass and activity, the biofilm micromorphology as well as the composition and functional groups characteristics of extracellular polymeric substances (EPS) in biofilm were investigated in laboratory-scale fixed bed biofilm reactors.
27148706	1	22	from	biofilm	307:313	arg1	groups					237:242	functional groups	226:242	functional groups	226:242	The effects of Fe(III) on the biofilm mass and activity, the biofilm micromorphology as well as the composition and functional groups characteristics of extracellular polymeric substances (EPS) in biofilm were investigated in laboratory-scale fixed bed biofilm reactors.
27148706	1	22	from	biofilm	307:313	arg1	activity					157:164	activity	157:164	activity	157:164	The effects of Fe(III) on the biofilm mass and activity, the biofilm micromorphology as well as the composition and functional groups characteristics of extracellular polymeric substances (EPS) in biofilm were investigated in laboratory-scale fixed bed biofilm reactors.
27148706	1	22	from	biofilm	307:313	arg1	EPS					299:301	EPS	299:301	EPS	299:301	The effects of Fe(III) on the biofilm mass and activity, the biofilm micromorphology as well as the composition and functional groups characteristics of extracellular polymeric substances (EPS) in biofilm were investigated in laboratory-scale fixed bed biofilm reactors.
27148706	1	22	from	biofilm	307:313	arg1	composition					210:220	composition	210:220	composition	210:220	The effects of Fe(III) on the biofilm mass and activity, the biofilm micromorphology as well as the composition and functional groups characteristics of extracellular polymeric substances (EPS) in biofilm were investigated in laboratory-scale fixed bed biofilm reactors.
27148706	1	22	from	biofilm	307:313	arg1	substances					287:296	extracellular polymeric substances	263:296	extracellular polymeric substances (EPS) in biofilm	263:313	The effects of Fe(III) on the biofilm mass and activity, the biofilm micromorphology as well as the composition and functional groups characteristics of extracellular polymeric substances (EPS) in biofilm were investigated in laboratory-scale fixed bed biofilm reactors.
27148706	1	22	from	biofilm	307:313	arg1	micromorphology					179:193	the biofilm micromorphology	167:193	the biofilm micromorphology	167:193	The effects of Fe(III) on the biofilm mass and activity, the biofilm micromorphology as well as the composition and functional groups characteristics of extracellular polymeric substances (EPS) in biofilm were investigated in laboratory-scale fixed bed biofilm reactors.
27148706	6	23	theme	theoretical	1422:1432	arg1	guidance					1434:1441	theoretical guidance	1422:1441	theoretical guidance for iron ion containing wastewater treatment	1422:1486	This study reveals that Fe(III) influences biofilm development and activity not only by directly impacting the microbial physiology but by indirectly affecting the EPS constituents, and it helps to provide theoretical guidance for iron ion containing wastewater treatment.
27148706	4	24	theme	microbial	865:873	arg1	aggregation					875:885	the microbial aggregation	861:885	the microbial aggregation	861:885	The result of the EPS composition suggested 2 mg/L of Fe(III) increased soluble EPS and loosely bound EPS which contributed to the microbial aggregation, while 16 mg/L of Fe(III) promoted tightly bound EPS production unfavourable for substrate mass transfer.
27148706	5	25	theme	-CONH-	1144:1149	arg1	polysaccharides					1192:1206	polysaccharides	1192:1206	polysaccharides in EPS	1192:1213	Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy analysis demonstrated that Fe(III) exerted a significant influence on the -CONH- groups of proteins and the C-O groups of polysaccharides in EPS.
27148706	5	25	theme	-CONH-	1144:1149	arg1	proteins					1161:1168	proteins	1161:1168	proteins	1161:1168	Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy analysis demonstrated that Fe(III) exerted a significant influence on the -CONH- groups of proteins and the C-O groups of polysaccharides in EPS.
27148706	5	25	theme	-CONH-	1144:1149	arg1	groups					1151:1156	the -CONH- groups	1140:1156	the -CONH- groups of proteins	1140:1168	Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy analysis demonstrated that Fe(III) exerted a significant influence on the -CONH- groups of proteins and the C-O groups of polysaccharides in EPS.
27148706	1	26	from	composition	210:220	arg1	biofilm					307:313	biofilm	307:313	biofilm	307:313	The effects of Fe(III) on the biofilm mass and activity, the biofilm micromorphology as well as the composition and functional groups characteristics of extracellular polymeric substances (EPS) in biofilm were investigated in laboratory-scale fixed bed biofilm reactors.
27148706	5	27	theme	X-ray	1037:1041	arg1	spectroscopy					1057:1068	X-ray photoelectron spectroscopy	1037:1068	X-ray photoelectron spectroscopy	1037:1068	Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy analysis demonstrated that Fe(III) exerted a significant influence on the -CONH- groups of proteins and the C-O groups of polysaccharides in EPS.
27148706	1	28	dep	composition	210:220	arg1	the					206:208	the	206:208	the	206:208	The effects of Fe(III) on the biofilm mass and activity, the biofilm micromorphology as well as the composition and functional groups characteristics of extracellular polymeric substances (EPS) in biofilm were investigated in laboratory-scale fixed bed biofilm reactors.
27148706	1	28	dep	composition	210:220	arg1	characteristics					244:258	characteristics	244:258	characteristics	244:258	The effects of Fe(III) on the biofilm mass and activity, the biofilm micromorphology as well as the composition and functional groups characteristics of extracellular polymeric substances (EPS) in biofilm were investigated in laboratory-scale fixed bed biofilm reactors.
27148706	1	29	theme	Fe	125:126	arg1	effects					114:120	The effects	110:120	The effects of Fe(III) on the biofilm mass and activity, the biofilm micromorphology as well as the composition and functional groups characteristics of extracellular polymeric substances (EPS) in biofilm	110:313	The effects of Fe(III) on the biofilm mass and activity, the biofilm micromorphology as well as the composition and functional groups characteristics of extracellular polymeric substances (EPS) in biofilm were investigated in laboratory-scale fixed bed biofilm reactors.
27148706	3	30	theme	high	600:603	arg1	concentration					605:617	a high concentration	598:617	a high concentration (16 mg/L) of Fe(III) led to significant reduction of the filaments, great promotion of the EPS secretion in biofilm	598:731	Scanning electron microscopy (SEM) study indicated a high concentration (16 mg/L) of Fe(III) led to significant reduction of the filaments, great promotion of the EPS secretion in biofilm.
27148706	3	30	theme	high	600:603	arg1	mg/L					623:626	16 mg/L	620:626	16 mg/L	620:626	Scanning electron microscopy (SEM) study indicated a high concentration (16 mg/L) of Fe(III) led to significant reduction of the filaments, great promotion of the EPS secretion in biofilm.
27148706	4	31	theme	EPS	752:754	arg1	composition					756:766	the EPS composition	748:766	the EPS composition	748:766	The result of the EPS composition suggested 2 mg/L of Fe(III) increased soluble EPS and loosely bound EPS which contributed to the microbial aggregation, while 16 mg/L of Fe(III) promoted tightly bound EPS production unfavourable for substrate mass transfer.
27148706	5	32	from	EPS	1211:1213	arg1	polysaccharides					1192:1206	polysaccharides	1192:1206	polysaccharides in EPS	1192:1213	Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy analysis demonstrated that Fe(III) exerted a significant influence on the -CONH- groups of proteins and the C-O groups of polysaccharides in EPS.
27148706	5	32	from	EPS	1211:1213	arg1	proteins					1161:1168	proteins	1161:1168	proteins	1161:1168	Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy analysis demonstrated that Fe(III) exerted a significant influence on the -CONH- groups of proteins and the C-O groups of polysaccharides in EPS.
27148706	5	32	from	EPS	1211:1213	arg1	groups					1151:1156	the -CONH- groups	1140:1156	the -CONH- groups of proteins	1140:1168	Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy analysis demonstrated that Fe(III) exerted a significant influence on the -CONH- groups of proteins and the C-O groups of polysaccharides in EPS.
27148706	5	32	from	EPS	1211:1213	arg1	groups					1182:1187	the C-O groups	1174:1187	the C-O groups of polysaccharides in EPS	1174:1213	Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy analysis demonstrated that Fe(III) exerted a significant influence on the -CONH- groups of proteins and the C-O groups of polysaccharides in EPS.
27148706	4	33	theme	bound	830:834	arg1	EPS					836:838	loosely bound EPS	822:838	loosely bound EPS	822:838	The result of the EPS composition suggested 2 mg/L of Fe(III) increased soluble EPS and loosely bound EPS which contributed to the microbial aggregation, while 16 mg/L of Fe(III) promoted tightly bound EPS production unfavourable for substrate mass transfer.
27148706	2	34	theme	Fe	499:500	arg1	mg/L					491:494	16 mg/L	488:494	16 mg/L of Fe(III)	488:505	The results showed that 2 mg/L of Fe(III) promoted the biofilm mass and improved the biofilm activity, but 16 mg/L of Fe(III) adversely affected biofilm development.
27148706	0	35	from	substances	62:71	arg1	reactors					100:107	fixed bed biofilm reactors	82:107	fixed bed biofilm reactors	82:107	Effects of Fe(III) on biofilm and its extracellular polymeric substances (EPS) in fixed bed biofilm reactors.
27148706	3	36	theme	microscopy	565:574	arg1	study					582:586	Scanning electron microscopy (SEM) study	547:586	Scanning electron microscopy (SEM) study	547:586	Scanning electron microscopy (SEM) study indicated a high concentration (16 mg/L) of Fe(III) led to significant reduction of the filaments, great promotion of the EPS secretion in biofilm.
27148706	3	37	from	promotion	691:699	arg1	biofilm					725:731	biofilm	725:731	biofilm	725:731	Scanning electron microscopy (SEM) study indicated a high concentration (16 mg/L) of Fe(III) led to significant reduction of the filaments, great promotion of the EPS secretion in biofilm.
27148706	6	38	theme	iron	1447:1450	arg1	treatment					1478:1486	iron ion containing wastewater treatment	1447:1486	iron ion containing wastewater treatment	1447:1486	This study reveals that Fe(III) influences biofilm development and activity not only by directly impacting the microbial physiology but by indirectly affecting the EPS constituents, and it helps to provide theoretical guidance for iron ion containing wastewater treatment.
27148706	0	39	theme	fixed	82:86	arg1	reactors					100:107	fixed bed biofilm reactors	82:107	fixed bed biofilm reactors	82:107	Effects of Fe(III) on biofilm and its extracellular polymeric substances (EPS) in fixed bed biofilm reactors.
27148706	6	40	theme	containing	1456:1465	arg1	treatment					1478:1486	iron ion containing wastewater treatment	1447:1486	iron ion containing wastewater treatment	1447:1486	This study reveals that Fe(III) influences biofilm development and activity not only by directly impacting the microbial physiology but by indirectly affecting the EPS constituents, and it helps to provide theoretical guidance for iron ion containing wastewater treatment.
27148706	5	41	theme	significant	1115:1125	arg1	influence					1127:1135	a significant influence	1113:1135	a significant influence on the -CONH- groups of proteins and the C-O groups of polysaccharides in EPS	1113:1213	Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy analysis demonstrated that Fe(III) exerted a significant influence on the -CONH- groups of proteins and the C-O groups of polysaccharides in EPS.
27148706	5	42	from	polysaccharides	1192:1206	arg1	EPS					1211:1213	EPS	1211:1213	EPS	1211:1213	Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy analysis demonstrated that Fe(III) exerted a significant influence on the -CONH- groups of proteins and the C-O groups of polysaccharides in EPS.
27148706	0	43	theme	Fe	11:12	arg1	Effects					0:6	Effects	0:6	Effects of Fe(III) on biofilm and its extracellular polymeric substances (EPS) in fixed bed biofilm reactors.	0:108	Effects of Fe(III) on biofilm and its extracellular polymeric substances (EPS) in fixed bed biofilm reactors.
27148706	4	44	theme	composition	756:766	arg1	result					738:743	The result	734:743	The result of the EPS composition	734:766	The result of the EPS composition suggested 2 mg/L of Fe(III) increased soluble EPS and loosely bound EPS which contributed to the microbial aggregation, while 16 mg/L of Fe(III) promoted tightly bound EPS production unfavourable for substrate mass transfer.
27148706	4	45	theme	Fe	905:906	arg1	mg/L					897:900	16 mg/L	894:900	16 mg/L of Fe(III)	894:911	The result of the EPS composition suggested 2 mg/L of Fe(III) increased soluble EPS and loosely bound EPS which contributed to the microbial aggregation, while 16 mg/L of Fe(III) promoted tightly bound EPS production unfavourable for substrate mass transfer.
27148706	3	46	theme	electron	556:563	arg1	SEM					577:579	SEM	577:579	SEM	577:579	Scanning electron microscopy (SEM) study indicated a high concentration (16 mg/L) of Fe(III) led to significant reduction of the filaments, great promotion of the EPS secretion in biofilm.
27148706	3	46	theme	electron	556:563	arg1	microscopy					565:574	Scanning electron microscopy	547:574	Scanning electron microscopy (SEM) study	547:586	Scanning electron microscopy (SEM) study indicated a high concentration (16 mg/L) of Fe(III) led to significant reduction of the filaments, great promotion of the EPS secretion in biofilm.
27148706	0	47	theme	biofilm	92:98	arg1	reactors					100:107	fixed bed biofilm reactors	82:107	fixed bed biofilm reactors	82:107	Effects of Fe(III) on biofilm and its extracellular polymeric substances (EPS) in fixed bed biofilm reactors.
27148706	5	48	from	influence	1127:1135	arg1	polysaccharides					1192:1206	polysaccharides	1192:1206	polysaccharides in EPS	1192:1213	Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy analysis demonstrated that Fe(III) exerted a significant influence on the -CONH- groups of proteins and the C-O groups of polysaccharides in EPS.
27148706	5	48	from	influence	1127:1135	arg1	proteins					1161:1168	proteins	1161:1168	proteins	1161:1168	Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy analysis demonstrated that Fe(III) exerted a significant influence on the -CONH- groups of proteins and the C-O groups of polysaccharides in EPS.
27148706	5	48	from	influence	1127:1135	arg1	groups					1151:1156	the -CONH- groups	1140:1156	the -CONH- groups of proteins	1140:1168	Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy analysis demonstrated that Fe(III) exerted a significant influence on the -CONH- groups of proteins and the C-O groups of polysaccharides in EPS.
27148706	5	48	from	influence	1127:1135	arg1	groups					1182:1187	the C-O groups	1174:1187	the C-O groups of polysaccharides in EPS	1174:1213	Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy analysis demonstrated that Fe(III) exerted a significant influence on the -CONH- groups of proteins and the C-O groups of polysaccharides in EPS.
27148706	6	49	theme	ion	1452:1454	arg1	treatment					1478:1486	iron ion containing wastewater treatment	1447:1486	iron ion containing wastewater treatment	1447:1486	This study reveals that Fe(III) influences biofilm development and activity not only by directly impacting the microbial physiology but by indirectly affecting the EPS constituents, and it helps to provide theoretical guidance for iron ion containing wastewater treatment.
27148706	5	50	from	groups	1182:1187	arg1	EPS					1211:1213	EPS	1211:1213	EPS	1211:1213	Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy analysis demonstrated that Fe(III) exerted a significant influence on the -CONH- groups of proteins and the C-O groups of polysaccharides in EPS.
27148706	1	51	from	substances	287:296	arg1	biofilm					307:313	biofilm	307:313	biofilm	307:313	The effects of Fe(III) on the biofilm mass and activity, the biofilm micromorphology as well as the composition and functional groups characteristics of extracellular polymeric substances (EPS) in biofilm were investigated in laboratory-scale fixed bed biofilm reactors.
27148706	6	52	dep	influences	1248:1257	arg1	affecting					1366:1374	affecting	1366:1374	indirectly affecting the EPS constituents	1355:1395	This study reveals that Fe(III) influences biofilm development and activity not only by directly impacting the microbial physiology but by indirectly affecting the EPS constituents, and it helps to provide theoretical guidance for iron ion containing wastewater treatment.
27148706	6	52	dep	influences	1248:1257	arg1	impacting					1313:1321	impacting	1313:1321	directly impacting the microbial physiology	1304:1346	This study reveals that Fe(III) influences biofilm development and activity not only by directly impacting the microbial physiology but by indirectly affecting the EPS constituents, and it helps to provide theoretical guidance for iron ion containing wastewater treatment.
27148706	0	53	theme	bed	88:90	arg1	reactors					100:107	fixed bed biofilm reactors	82:107	fixed bed biofilm reactors	82:107	Effects of Fe(III) on biofilm and its extracellular polymeric substances (EPS) in fixed bed biofilm reactors.
27148706	1	54	dep	mass	148:151	arg1	the					136:138	the	136:138	the	136:138	The effects of Fe(III) on the biofilm mass and activity, the biofilm micromorphology as well as the composition and functional groups characteristics of extracellular polymeric substances (EPS) in biofilm were investigated in laboratory-scale fixed bed biofilm reactors.
27148706	4	55	theme	mass	978:981	arg1	transfer					983:990	substrate mass transfer	968:990	substrate mass transfer	968:990	The result of the EPS composition suggested 2 mg/L of Fe(III) increased soluble EPS and loosely bound EPS which contributed to the microbial aggregation, while 16 mg/L of Fe(III) promoted tightly bound EPS production unfavourable for substrate mass transfer.
27148706	1	56	theme	biofilm	140:146	arg1	mass					148:151	biofilm mass	140:151	biofilm mass	140:151	The effects of Fe(III) on the biofilm mass and activity, the biofilm micromorphology as well as the composition and functional groups characteristics of extracellular polymeric substances (EPS) in biofilm were investigated in laboratory-scale fixed bed biofilm reactors.
27148706	1	57	theme	functional	226:235	arg1	EPS					299:301	EPS	299:301	EPS	299:301	The effects of Fe(III) on the biofilm mass and activity, the biofilm micromorphology as well as the composition and functional groups characteristics of extracellular polymeric substances (EPS) in biofilm were investigated in laboratory-scale fixed bed biofilm reactors.
27148706	1	57	theme	functional	226:235	arg1	substances					287:296	extracellular polymeric substances	263:296	extracellular polymeric substances (EPS) in biofilm	263:313	The effects of Fe(III) on the biofilm mass and activity, the biofilm micromorphology as well as the composition and functional groups characteristics of extracellular polymeric substances (EPS) in biofilm were investigated in laboratory-scale fixed bed biofilm reactors.
27148706	1	57	theme	functional	226:235	arg1	groups					237:242	functional groups	226:242	functional groups	226:242	The effects of Fe(III) on the biofilm mass and activity, the biofilm micromorphology as well as the composition and functional groups characteristics of extracellular polymeric substances (EPS) in biofilm were investigated in laboratory-scale fixed bed biofilm reactors.
27148706	1	58	from	activity	157:164	arg1	biofilm					307:313	biofilm	307:313	biofilm	307:313	The effects of Fe(III) on the biofilm mass and activity, the biofilm micromorphology as well as the composition and functional groups characteristics of extracellular polymeric substances (EPS) in biofilm were investigated in laboratory-scale fixed bed biofilm reactors.
27148706	4	59	theme	substrate	968:976	arg1	transfer					983:990	substrate mass transfer	968:990	substrate mass transfer	968:990	The result of the EPS composition suggested 2 mg/L of Fe(III) increased soluble EPS and loosely bound EPS which contributed to the microbial aggregation, while 16 mg/L of Fe(III) promoted tightly bound EPS production unfavourable for substrate mass transfer.
27148706	0	60	from	Effects	0:6	arg1	EPS					74:76	EPS	74:76	EPS	74:76	Effects of Fe(III) on biofilm and its extracellular polymeric substances (EPS) in fixed bed biofilm reactors.
27148706	0	60	from	Effects	0:6	arg1	substances					62:71	its extracellular polymeric substances	34:71	its extracellular polymeric substances (EPS)	34:77	Effects of Fe(III) on biofilm and its extracellular polymeric substances (EPS) in fixed bed biofilm reactors.
27148706	0	60	from	Effects	0:6	arg1	biofilm					22:28	biofilm	22:28	biofilm	22:28	Effects of Fe(III) on biofilm and its extracellular polymeric substances (EPS) in fixed bed biofilm reactors.
27148706	3	61	theme	Fe	632:633	arg1	concentration					605:617	a high concentration	598:617	a high concentration (16 mg/L) of Fe(III) led to significant reduction of the filaments, great promotion of the EPS secretion in biofilm	598:731	Scanning electron microscopy (SEM) study indicated a high concentration (16 mg/L) of Fe(III) led to significant reduction of the filaments, great promotion of the EPS secretion in biofilm.
27148706	3	61	theme	Fe	632:633	arg1	mg/L					623:626	16 mg/L	620:626	16 mg/L	620:626	Scanning electron microscopy (SEM) study indicated a high concentration (16 mg/L) of Fe(III) led to significant reduction of the filaments, great promotion of the EPS secretion in biofilm.
27148706	1	62	from	micromorphology	179:193	arg1	biofilm					307:313	biofilm	307:313	biofilm	307:313	The effects of Fe(III) on the biofilm mass and activity, the biofilm micromorphology as well as the composition and functional groups characteristics of extracellular polymeric substances (EPS) in biofilm were investigated in laboratory-scale fixed bed biofilm reactors.
27148706	1	63	theme	laboratory-scale	336:351	arg1	reactors					371:378	laboratory-scale fixed bed biofilm reactors	336:378	laboratory-scale fixed bed biofilm reactors	336:378	The effects of Fe(III) on the biofilm mass and activity, the biofilm micromorphology as well as the composition and functional groups characteristics of extracellular polymeric substances (EPS) in biofilm were investigated in laboratory-scale fixed bed biofilm reactors.
27148706	5	64	dep	spectroscopy	1020:1031	arg1	analysis					1070:1077	analysis	1070:1077	analysis	1070:1077	Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy analysis demonstrated that Fe(III) exerted a significant influence on the -CONH- groups of proteins and the C-O groups of polysaccharides in EPS.
27148706	5	65	theme	polysaccharides	1192:1206	arg1	polysaccharides					1192:1206	polysaccharides	1192:1206	polysaccharides in EPS	1192:1213	Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy analysis demonstrated that Fe(III) exerted a significant influence on the -CONH- groups of proteins and the C-O groups of polysaccharides in EPS.
27148706	5	65	theme	polysaccharides	1192:1206	arg1	proteins					1161:1168	proteins	1161:1168	proteins	1161:1168	Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy analysis demonstrated that Fe(III) exerted a significant influence on the -CONH- groups of proteins and the C-O groups of polysaccharides in EPS.
27148706	5	65	theme	polysaccharides	1192:1206	arg1	groups					1151:1156	the -CONH- groups	1140:1156	the -CONH- groups of proteins	1140:1168	Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy analysis demonstrated that Fe(III) exerted a significant influence on the -CONH- groups of proteins and the C-O groups of polysaccharides in EPS.
27148706	5	65	theme	polysaccharides	1192:1206	arg1	groups					1182:1187	the C-O groups	1174:1187	the C-O groups of polysaccharides in EPS	1174:1213	Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy analysis demonstrated that Fe(III) exerted a significant influence on the -CONH- groups of proteins and the C-O groups of polysaccharides in EPS.
27148706	4	66	theme	Fe	788:789	arg1	mg/L					780:783	2 mg/L	778:783	2 mg/L of Fe(III)	778:794	The result of the EPS composition suggested 2 mg/L of Fe(III) increased soluble EPS and loosely bound EPS which contributed to the microbial aggregation, while 16 mg/L of Fe(III) promoted tightly bound EPS production unfavourable for substrate mass transfer.
27148706	1	67	from	mass	148:151	arg1	biofilm					307:313	biofilm	307:313	biofilm	307:313	The effects of Fe(III) on the biofilm mass and activity, the biofilm micromorphology as well as the composition and functional groups characteristics of extracellular polymeric substances (EPS) in biofilm were investigated in laboratory-scale fixed bed biofilm reactors.
27148706	1	68	theme	fixed	353:357	arg1	reactors					371:378	laboratory-scale fixed bed biofilm reactors	336:378	laboratory-scale fixed bed biofilm reactors	336:378	The effects of Fe(III) on the biofilm mass and activity, the biofilm micromorphology as well as the composition and functional groups characteristics of extracellular polymeric substances (EPS) in biofilm were investigated in laboratory-scale fixed bed biofilm reactors.
27148706	5	69	from	groups	1151:1156	arg1	EPS					1211:1213	EPS	1211:1213	EPS	1211:1213	Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy analysis demonstrated that Fe(III) exerted a significant influence on the -CONH- groups of proteins and the C-O groups of polysaccharides in EPS.
27148706	6	70	theme	EPS	1380:1382	arg1	constituents					1384:1395	the EPS constituents	1376:1395	the EPS constituents	1376:1395	This study reveals that Fe(III) influences biofilm development and activity not only by directly impacting the microbial physiology but by indirectly affecting the EPS constituents, and it helps to provide theoretical guidance for iron ion containing wastewater treatment.
27148706	5	71	theme	C-O	1178:1180	arg1	proteins					1161:1168	proteins	1161:1168	proteins	1161:1168	Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy analysis demonstrated that Fe(III) exerted a significant influence on the -CONH- groups of proteins and the C-O groups of polysaccharides in EPS.
27148706	5	71	theme	C-O	1178:1180	arg1	polysaccharides					1192:1206	polysaccharides	1192:1206	polysaccharides in EPS	1192:1213	Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy analysis demonstrated that Fe(III) exerted a significant influence on the -CONH- groups of proteins and the C-O groups of polysaccharides in EPS.
27148706	5	71	theme	C-O	1178:1180	arg1	groups					1182:1187	the C-O groups	1174:1187	the C-O groups of polysaccharides in EPS	1174:1213	Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy analysis demonstrated that Fe(III) exerted a significant influence on the -CONH- groups of proteins and the C-O groups of polysaccharides in EPS.
27148706	3	72	theme	significant	647:656	arg1	reduction					658:666	significant reduction	647:666	significant reduction of the filaments, great promotion of the EPS secretion in biofilm	647:731	Scanning electron microscopy (SEM) study indicated a high concentration (16 mg/L) of Fe(III) led to significant reduction of the filaments, great promotion of the EPS secretion in biofilm.
27148706	2	73	theme	biofilm	466:472	arg1	activity					474:481	the biofilm activity	462:481	the biofilm activity	462:481	The results showed that 2 mg/L of Fe(III) promoted the biofilm mass and improved the biofilm activity, but 16 mg/L of Fe(III) adversely affected biofilm development.
27148706	1	74	theme	bed	359:361	arg1	reactors					371:378	laboratory-scale fixed bed biofilm reactors	336:378	laboratory-scale fixed bed biofilm reactors	336:378	The effects of Fe(III) on the biofilm mass and activity, the biofilm micromorphology as well as the composition and functional groups characteristics of extracellular polymeric substances (EPS) in biofilm were investigated in laboratory-scale fixed bed biofilm reactors.
27148706	5	75	theme	proteins	1161:1168	arg1	polysaccharides					1192:1206	polysaccharides	1192:1206	polysaccharides in EPS	1192:1213	Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy analysis demonstrated that Fe(III) exerted a significant influence on the -CONH- groups of proteins and the C-O groups of polysaccharides in EPS.
27148706	5	75	theme	proteins	1161:1168	arg1	proteins					1161:1168	proteins	1161:1168	proteins	1161:1168	Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy analysis demonstrated that Fe(III) exerted a significant influence on the -CONH- groups of proteins and the C-O groups of polysaccharides in EPS.
27148706	5	75	theme	proteins	1161:1168	arg1	groups					1151:1156	the -CONH- groups	1140:1156	the -CONH- groups of proteins	1140:1168	Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy analysis demonstrated that Fe(III) exerted a significant influence on the -CONH- groups of proteins and the C-O groups of polysaccharides in EPS.
27148706	5	75	theme	proteins	1161:1168	arg1	groups					1182:1187	the C-O groups	1174:1187	the C-O groups of polysaccharides in EPS	1174:1213	Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy analysis demonstrated that Fe(III) exerted a significant influence on the -CONH- groups of proteins and the C-O groups of polysaccharides in EPS.
27148706	3	76	theme	great	685:689	arg1	filaments					675:682	the filaments	671:682	the filaments	671:682	Scanning electron microscopy (SEM) study indicated a high concentration (16 mg/L) of Fe(III) led to significant reduction of the filaments, great promotion of the EPS secretion in biofilm.
27148706	3	76	theme	great	685:689	arg1	promotion					691:699	great promotion	685:699	great promotion of the EPS secretion in biofilm	685:731	Scanning electron microscopy (SEM) study indicated a high concentration (16 mg/L) of Fe(III) led to significant reduction of the filaments, great promotion of the EPS secretion in biofilm.
27148706	4	77	theme	bound	930:934	arg1	production					940:949	tightly bound EPS production	922:949	tightly bound EPS production unfavourable for substrate mass transfer	922:990	The result of the EPS composition suggested 2 mg/L of Fe(III) increased soluble EPS and loosely bound EPS which contributed to the microbial aggregation, while 16 mg/L of Fe(III) promoted tightly bound EPS production unfavourable for substrate mass transfer.
27148706	1	78	theme	biofilm	363:369	arg1	reactors					371:378	laboratory-scale fixed bed biofilm reactors	336:378	laboratory-scale fixed bed biofilm reactors	336:378	The effects of Fe(III) on the biofilm mass and activity, the biofilm micromorphology as well as the composition and functional groups characteristics of extracellular polymeric substances (EPS) in biofilm were investigated in laboratory-scale fixed bed biofilm reactors.
27148706	4	79	theme	EPS	936:938	arg1	production					940:949	tightly bound EPS production	922:949	tightly bound EPS production unfavourable for substrate mass transfer	922:990	The result of the EPS composition suggested 2 mg/L of Fe(III) increased soluble EPS and loosely bound EPS which contributed to the microbial aggregation, while 16 mg/L of Fe(III) promoted tightly bound EPS production unfavourable for substrate mass transfer.
27148706	4	80	dep	suggested	768:776	arg1	increased					796:804	increased	796:804	suggested 2 mg/L of Fe(III) increased soluble EPS and loosely bound EPS which contributed to the microbial aggregation, while 16 mg/L of Fe(III) promoted tightly bound EPS production unfavourable for substrate mass transfer	768:990	The result of the EPS composition suggested 2 mg/L of Fe(III) increased soluble EPS and loosely bound EPS which contributed to the microbial aggregation, while 16 mg/L of Fe(III) promoted tightly bound EPS production unfavourable for substrate mass transfer.
27148706	5	81	theme	photoelectron	1043:1055	arg1	spectroscopy					1057:1068	X-ray photoelectron spectroscopy	1037:1068	X-ray photoelectron spectroscopy	1037:1068	Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy analysis demonstrated that Fe(III) exerted a significant influence on the -CONH- groups of proteins and the C-O groups of polysaccharides in EPS.
27148706	6	82	theme	biofilm	1259:1265	arg1	development					1267:1277	biofilm development	1259:1277	biofilm development	1259:1277	This study reveals that Fe(III) influences biofilm development and activity not only by directly impacting the microbial physiology but by indirectly affecting the EPS constituents, and it helps to provide theoretical guidance for iron ion containing wastewater treatment.
28385239	10	0	theme	potential	1552:1560	arg1	membrane					1523:1530	the obtained membrane	1510:1530	the obtained membrane	1510:1530	Therefore, the obtained membrane was considered as a potential candidate for wound dressing materials.
28385239	10	0	theme	potential	1552:1560	arg1	candidate					1562:1570	a potential candidate	1550:1570	a potential candidate for wound dressing materials	1550:1599	Therefore, the obtained membrane was considered as a potential candidate for wound dressing materials.
28385239	4	1	theme	water	730:734	arg1	rate					755:758	water vapor transmission rate	730:758	water vapor transmission rate	730:758	In addition, tensile strength, transparence, water absorption, water retention, water vapor transmission rate, adsorption of BSA, and antibacterial properties were thoroughly investigated.
28385239	6	2	theme	absorption	1109:1118	arg1	capacity					1140:1147	the water absorption and water retention capacity	1099:1147	the water absorption and water retention capacity of the membrane	1099:1163	Moreover, the incorporation enhanced the tensile strength and the water absorption and water retention capacity of the membrane.
28385239	5	3	dep	strengthened	888:899	arg1	omitted					955:961	omitted	955:961	omitted the any subsequent crosslinking processes between chitosan and cellulose	955:1034	Incorporation of citrate-modified nano-maghemite strengthened the binding of chitosan onto cellulose membranes thus omitted the any subsequent crosslinking processes between chitosan and cellulose.
28385239	8	4	theme	ideal	1321:1325	arg1	dressings					1333:1341	ideal wound dressings	1321:1341	ideal wound dressings	1321:1341	The water vapor transmission rate was determined and exhibited suitable for ideal wound dressings.
28385239	2	5	theme	wound	411:415	arg1	dressings					417:425	wound dressings	411:425	wound dressings	411:425	Nano-maghemite-incorporated chitosan-coated cellulose porous membranes were prepared and evaluated in terms of those critical requirements as wound dressings.
28385239	8	6	theme	vapor	1255:1259	arg1	rate					1274:1277	The water vapor transmission rate	1245:1277	The water vapor transmission rate	1245:1277	The water vapor transmission rate was determined and exhibited suitable for ideal wound dressings.
28385239	7	7	theme	cellulose	1197:1205	arg1	membranes					1207:1215	cellulose membranes	1197:1215	cellulose membranes	1197:1215	Chitosan was tightly coated on cellulose membranes without blocking the pores.
28385239	3	8	dep	transform	541:549	arg1	infrared					551:558	infrared	551:558	transform infrared spectroscopy (FTIR)	541:578	The composite membranes were characterized using elemental analysis, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffractometry (XRD), and vibrating sample magnetometer (VSM).
28385239	6	9	theme	tensile	1078:1084	arg1	strength					1086:1093	the tensile strength	1074:1093	the tensile strength	1074:1093	Moreover, the incorporation enhanced the tensile strength and the water absorption and water retention capacity of the membrane.
28385239	0	10	theme	potential	98:106	arg1	application					108:118	potential application	98:118	potential application as wound dressing	98:136	Citrate-modified maghemite enhanced binding of chitosan coating on cellulose porous membranes for potential application as wound dressing.
28385239	9	11	theme	chitosan	1414:1421	arg1	coating					1403:1409	the coating	1399:1409	the coating of chitosan	1399:1421	Especially, the antibacterial evaluation revealed that the coating of chitosan significantly enhanced the growth inhibition of both S. aureus and E. coli.
28385239	0	12	theme	wound	123:127	arg1	dressing					129:136	wound dressing	123:136	wound dressing	123:136	Citrate-modified maghemite enhanced binding of chitosan coating on cellulose porous membranes for potential application as wound dressing.
28385239	5	13	theme	crosslinking	982:993	arg1	processes					995:1003	any subsequent crosslinking processes	967:1003	the any subsequent crosslinking processes between chitosan and cellulose	963:1034	Incorporation of citrate-modified nano-maghemite strengthened the binding of chitosan onto cellulose membranes thus omitted the any subsequent crosslinking processes between chitosan and cellulose.
28385239	10	14	theme	dressing	1582:1589	arg1	materials					1591:1599	wound dressing materials	1576:1599	wound dressing materials	1576:1599	Therefore, the obtained membrane was considered as a potential candidate for wound dressing materials.
28385239	1	15	theme	chemical	197:204	arg1	processes					206:214	chemical processes	197:214	chemical processes	197:214	To fabricate ideal wound dressings without involvement of chemical processes is attracting the interest in clinical requirements.
28385239	4	16	theme	water	695:699	arg1	absorption					701:710	water absorption	695:710	water absorption	695:710	In addition, tensile strength, transparence, water absorption, water retention, water vapor transmission rate, adsorption of BSA, and antibacterial properties were thoroughly investigated.
28385239	8	17	theme	transmission	1261:1272	arg1	rate					1274:1277	The water vapor transmission rate	1245:1277	The water vapor transmission rate	1245:1277	The water vapor transmission rate was determined and exhibited suitable for ideal wound dressings.
28385239	5	18	theme	subsequent	971:980	arg1	processes					995:1003	any subsequent crosslinking processes	967:1003	the any subsequent crosslinking processes between chitosan and cellulose	963:1034	Incorporation of citrate-modified nano-maghemite strengthened the binding of chitosan onto cellulose membranes thus omitted the any subsequent crosslinking processes between chitosan and cellulose.
28385239	10	19	theme	wound	1576:1580	arg1	materials					1591:1599	wound dressing materials	1576:1599	wound dressing materials	1576:1599	Therefore, the obtained membrane was considered as a potential candidate for wound dressing materials.
28385239	5	20	theme	citrate-modified	856:871	arg1	nano-maghemite					873:886	citrate-modified nano-maghemite	856:886	citrate-modified nano-maghemite	856:886	Incorporation of citrate-modified nano-maghemite strengthened the binding of chitosan onto cellulose membranes thus omitted the any subsequent crosslinking processes between chitosan and cellulose.
28385239	5	21	theme	cellulose	930:938	arg1	membranes					940:948	cellulose membranes	930:948	cellulose membranes	930:948	Incorporation of citrate-modified nano-maghemite strengthened the binding of chitosan onto cellulose membranes thus omitted the any subsequent crosslinking processes between chitosan and cellulose.
28385239	3	22	theme	vibrating	613:621	arg1	VSM					644:646	VSM	644:646	VSM	644:646	The composite membranes were characterized using elemental analysis, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffractometry (XRD), and vibrating sample magnetometer (VSM).
28385239	3	22	theme	vibrating	613:621	arg1	magnetometer					630:641	vibrating sample magnetometer	613:641	vibrating sample magnetometer (VSM)	613:647	The composite membranes were characterized using elemental analysis, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffractometry (XRD), and vibrating sample magnetometer (VSM).
28385239	3	23	theme	scanning	497:504	arg1	microscopy					515:524	scanning electron microscopy	497:524	scanning electron microscopy (SEM)	497:530	The composite membranes were characterized using elemental analysis, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffractometry (XRD), and vibrating sample magnetometer (VSM).
28385239	3	23	theme	scanning	497:504	arg1	SEM					527:529	SEM	527:529	SEM	527:529	The composite membranes were characterized using elemental analysis, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffractometry (XRD), and vibrating sample magnetometer (VSM).
28385239	0	24	theme	Citrate-modified	0:15	arg1	maghemite					17:25	Citrate-modified maghemite	0:25	Citrate-modified maghemite	0:25	Citrate-modified maghemite enhanced binding of chitosan coating on cellulose porous membranes for potential application as wound dressing.
28385239	9	25	theme	growth	1450:1455	arg1	inhibition					1457:1466	the growth inhibition	1446:1466	the growth inhibition of both S. aureus and E. coli	1446:1496	Especially, the antibacterial evaluation revealed that the coating of chitosan significantly enhanced the growth inhibition of both S. aureus and E. coli.
28385239	10	26	theme	obtained	1514:1521	arg1	membrane					1523:1530	the obtained membrane	1510:1530	the obtained membrane	1510:1530	Therefore, the obtained membrane was considered as a potential candidate for wound dressing materials.
28385239	10	26	theme	obtained	1514:1521	arg1	candidate					1562:1570	a potential candidate	1550:1570	a potential candidate for wound dressing materials	1550:1599	Therefore, the obtained membrane was considered as a potential candidate for wound dressing materials.
28385239	3	27	theme	sample	623:628	arg1	VSM					644:646	VSM	644:646	VSM	644:646	The composite membranes were characterized using elemental analysis, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffractometry (XRD), and vibrating sample magnetometer (VSM).
28385239	3	27	theme	sample	623:628	arg1	magnetometer					630:641	vibrating sample magnetometer	613:641	vibrating sample magnetometer (VSM)	613:647	The composite membranes were characterized using elemental analysis, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffractometry (XRD), and vibrating sample magnetometer (VSM).
28385239	4	28	theme	antibacterial	784:796	arg1	properties					798:807	antibacterial properties	784:807	antibacterial properties	784:807	In addition, tensile strength, transparence, water absorption, water retention, water vapor transmission rate, adsorption of BSA, and antibacterial properties were thoroughly investigated.
28385239	3	29	theme	electron	506:513	arg1	microscopy					515:524	scanning electron microscopy	497:524	scanning electron microscopy (SEM)	497:530	The composite membranes were characterized using elemental analysis, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffractometry (XRD), and vibrating sample magnetometer (VSM).
28385239	3	29	theme	electron	506:513	arg1	SEM					527:529	SEM	527:529	SEM	527:529	The composite membranes were characterized using elemental analysis, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffractometry (XRD), and vibrating sample magnetometer (VSM).
28385239	3	30	theme	composite	432:440	arg1	membranes					442:450	The composite membranes	428:450	The composite membranes	428:450	The composite membranes were characterized using elemental analysis, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffractometry (XRD), and vibrating sample magnetometer (VSM).
28385239	4	31	theme	BSA	775:777	arg1	rate					755:758	water vapor transmission rate	730:758	water vapor transmission rate	730:758	In addition, tensile strength, transparence, water absorption, water retention, water vapor transmission rate, adsorption of BSA, and antibacterial properties were thoroughly investigated.
28385239	4	31	theme	BSA	775:777	arg1	strength					671:678	tensile strength	663:678	tensile strength	663:678	In addition, tensile strength, transparence, water absorption, water retention, water vapor transmission rate, adsorption of BSA, and antibacterial properties were thoroughly investigated.
28385239	4	31	theme	BSA	775:777	arg1	transparence					681:692	transparence	681:692	transparence	681:692	In addition, tensile strength, transparence, water absorption, water retention, water vapor transmission rate, adsorption of BSA, and antibacterial properties were thoroughly investigated.
28385239	4	31	theme	BSA	775:777	arg1	properties					798:807	antibacterial properties	784:807	antibacterial properties	784:807	In addition, tensile strength, transparence, water absorption, water retention, water vapor transmission rate, adsorption of BSA, and antibacterial properties were thoroughly investigated.
28385239	4	31	theme	BSA	775:777	arg1	retention					719:727	water retention	713:727	water retention	713:727	In addition, tensile strength, transparence, water absorption, water retention, water vapor transmission rate, adsorption of BSA, and antibacterial properties were thoroughly investigated.
28385239	4	31	theme	BSA	775:777	arg1	adsorption					761:770	adsorption	761:770	adsorption of BSA	761:777	In addition, tensile strength, transparence, water absorption, water retention, water vapor transmission rate, adsorption of BSA, and antibacterial properties were thoroughly investigated.
28385239	4	31	theme	BSA	775:777	arg1	absorption					701:710	water absorption	695:710	water absorption	695:710	In addition, tensile strength, transparence, water absorption, water retention, water vapor transmission rate, adsorption of BSA, and antibacterial properties were thoroughly investigated.
28385239	4	32	theme	tensile	663:669	arg1	strength					671:678	tensile strength	663:678	tensile strength	663:678	In addition, tensile strength, transparence, water absorption, water retention, water vapor transmission rate, adsorption of BSA, and antibacterial properties were thoroughly investigated.
28385239	0	33	theme	chitosan	47:54	arg1	binding					36:42	binding	36:42	binding of chitosan	36:54	Citrate-modified maghemite enhanced binding of chitosan coating on cellulose porous membranes for potential application as wound dressing.
28385239	4	34	theme	vapor	736:740	arg1	rate					755:758	water vapor transmission rate	730:758	water vapor transmission rate	730:758	In addition, tensile strength, transparence, water absorption, water retention, water vapor transmission rate, adsorption of BSA, and antibacterial properties were thoroughly investigated.
28385239	1	35	theme	processes	206:214	arg1	involvement					182:192	involvement	182:192	involvement of chemical processes	182:214	To fabricate ideal wound dressings without involvement of chemical processes is attracting the interest in clinical requirements.
28385239	6	36	theme	retention	1130:1138	arg1	capacity					1140:1147	the water absorption and water retention capacity	1099:1147	the water absorption and water retention capacity of the membrane	1099:1163	Moreover, the incorporation enhanced the tensile strength and the water absorption and water retention capacity of the membrane.
28385239	6	37	theme	membrane	1156:1163	arg1	strength					1086:1093	the tensile strength	1074:1093	the tensile strength	1074:1093	Moreover, the incorporation enhanced the tensile strength and the water absorption and water retention capacity of the membrane.
28385239	6	37	theme	membrane	1156:1163	arg1	capacity					1140:1147	the water absorption and water retention capacity	1099:1147	the water absorption and water retention capacity of the membrane	1099:1163	Moreover, the incorporation enhanced the tensile strength and the water absorption and water retention capacity of the membrane.
28385239	5	38	theme	nano-maghemite	873:886	arg1	Incorporation					839:851	Incorporation	839:851	Incorporation of citrate-modified nano-maghemite	839:886	Incorporation of citrate-modified nano-maghemite strengthened the binding of chitosan onto cellulose membranes thus omitted the any subsequent crosslinking processes between chitosan and cellulose.
28385239	5	39	theme	chitosan	916:923	arg1	binding					905:911	the binding	901:911	the binding of chitosan onto cellulose membranes	901:948	Incorporation of citrate-modified nano-maghemite strengthened the binding of chitosan onto cellulose membranes thus omitted the any subsequent crosslinking processes between chitosan and cellulose.
28385239	8	40	theme	wound	1327:1331	arg1	dressings					1333:1341	ideal wound dressings	1321:1341	ideal wound dressings	1321:1341	The water vapor transmission rate was determined and exhibited suitable for ideal wound dressings.
28385239	9	41	theme	aureus	1479:1484	arg1	inhibition					1457:1466	the growth inhibition	1446:1466	the growth inhibition of both S. aureus and E. coli	1446:1496	Especially, the antibacterial evaluation revealed that the coating of chitosan significantly enhanced the growth inhibition of both S. aureus and E. coli.
28385239	1	42	from	interest	234:241	arg1	requirements					255:266	clinical requirements	246:266	clinical requirements	246:266	To fabricate ideal wound dressings without involvement of chemical processes is attracting the interest in clinical requirements.
28385239	4	43	theme	transmission	742:753	arg1	rate					755:758	water vapor transmission rate	730:758	water vapor transmission rate	730:758	In addition, tensile strength, transparence, water absorption, water retention, water vapor transmission rate, adsorption of BSA, and antibacterial properties were thoroughly investigated.
28385239	3	44	theme	elemental	477:485	arg1	analysis					487:494	elemental analysis	477:494	elemental analysis	477:494	The composite membranes were characterized using elemental analysis, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffractometry (XRD), and vibrating sample magnetometer (VSM).
28385239	2	45	theme	requirements	395:406	arg1	terms					371:375	terms	371:375	terms of those critical requirements as wound dressings	371:425	Nano-maghemite-incorporated chitosan-coated cellulose porous membranes were prepared and evaluated in terms of those critical requirements as wound dressings.
28385239	3	46	dep	Fourier	533:539	arg1	transform					541:549	transform	541:549	transform infrared spectroscopy (FTIR)	541:578	The composite membranes were characterized using elemental analysis, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffractometry (XRD), and vibrating sample magnetometer (VSM).
28385239	9	47	theme	antibacterial	1360:1372	arg1	evaluation					1374:1383	the antibacterial evaluation	1356:1383	the antibacterial evaluation	1356:1383	Especially, the antibacterial evaluation revealed that the coating of chitosan significantly enhanced the growth inhibition of both S. aureus and E. coli.
28385239	2	48	theme	porous	323:328	arg1	membranes					330:338	Nano-maghemite-incorporated chitosan-coated cellulose porous membranes	269:338	Nano-maghemite-incorporated chitosan-coated cellulose porous membranes	269:338	Nano-maghemite-incorporated chitosan-coated cellulose porous membranes were prepared and evaluated in terms of those critical requirements as wound dressings.
28385239	4	49	theme	water	713:717	arg1	retention					719:727	water retention	713:727	water retention	713:727	In addition, tensile strength, transparence, water absorption, water retention, water vapor transmission rate, adsorption of BSA, and antibacterial properties were thoroughly investigated.
28385239	2	50	theme	critical	386:393	arg1	requirements					395:406	those critical requirements	380:406	those critical requirements as wound dressings	380:425	Nano-maghemite-incorporated chitosan-coated cellulose porous membranes were prepared and evaluated in terms of those critical requirements as wound dressings.
28385239	9	51	theme	coli	1493:1496	arg1	inhibition					1457:1466	the growth inhibition	1446:1466	the growth inhibition of both S. aureus and E. coli	1446:1496	Especially, the antibacterial evaluation revealed that the coating of chitosan significantly enhanced the growth inhibition of both S. aureus and E. coli.
28385239	2	52	theme	cellulose	313:321	arg1	membranes					330:338	Nano-maghemite-incorporated chitosan-coated cellulose porous membranes	269:338	Nano-maghemite-incorporated chitosan-coated cellulose porous membranes	269:338	Nano-maghemite-incorporated chitosan-coated cellulose porous membranes were prepared and evaluated in terms of those critical requirements as wound dressings.
28385239	1	53	theme	wound	158:162	arg1	dressings					164:172	ideal wound dressings	152:172	ideal wound dressings	152:172	To fabricate ideal wound dressings without involvement of chemical processes is attracting the interest in clinical requirements.
28385239	1	54	theme	clinical	246:253	arg1	requirements					255:266	clinical requirements	246:266	clinical requirements	246:266	To fabricate ideal wound dressings without involvement of chemical processes is attracting the interest in clinical requirements.
28385239	0	55	theme	porous	77:82	arg1	membranes					84:92	cellulose porous membranes	67:92	cellulose porous membranes for potential application as wound dressing	67:136	Citrate-modified maghemite enhanced binding of chitosan coating on cellulose porous membranes for potential application as wound dressing.
28385239	6	56	theme	water	1124:1128	arg1	retention					1130:1138	water retention	1124:1138	water retention	1124:1138	Moreover, the incorporation enhanced the tensile strength and the water absorption and water retention capacity of the membrane.
28385239	2	57	theme	chitosan-coated	297:311	arg1	membranes					330:338	Nano-maghemite-incorporated chitosan-coated cellulose porous membranes	269:338	Nano-maghemite-incorporated chitosan-coated cellulose porous membranes	269:338	Nano-maghemite-incorporated chitosan-coated cellulose porous membranes were prepared and evaluated in terms of those critical requirements as wound dressings.
28385239	1	58	theme	ideal	152:156	arg1	dressings					164:172	ideal wound dressings	152:172	ideal wound dressings	152:172	To fabricate ideal wound dressings without involvement of chemical processes is attracting the interest in clinical requirements.
28385239	6	59	theme	water	1103:1107	arg1	absorption					1109:1118	water absorption	1103:1118	water absorption	1103:1118	Moreover, the incorporation enhanced the tensile strength and the water absorption and water retention capacity of the membrane.
28385239	0	60	theme	cellulose	67:75	arg1	membranes					84:92	cellulose porous membranes	67:92	cellulose porous membranes for potential application as wound dressing	67:136	Citrate-modified maghemite enhanced binding of chitosan coating on cellulose porous membranes for potential application as wound dressing.
28385239	2	61	theme	Nano-maghemite-incorporated	269:295	arg1	membranes					330:338	Nano-maghemite-incorporated chitosan-coated cellulose porous membranes	269:338	Nano-maghemite-incorporated chitosan-coated cellulose porous membranes	269:338	Nano-maghemite-incorporated chitosan-coated cellulose porous membranes were prepared and evaluated in terms of those critical requirements as wound dressings.
28385239	3	62	theme	X-ray	581:585	arg1	XRD					603:605	XRD	603:605	XRD	603:605	The composite membranes were characterized using elemental analysis, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffractometry (XRD), and vibrating sample magnetometer (VSM).
28385239	3	62	theme	X-ray	581:585	arg1	diffractometry					587:600	X-ray diffractometry	581:600	X-ray diffractometry (XRD)	581:606	The composite membranes were characterized using elemental analysis, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffractometry (XRD), and vibrating sample magnetometer (VSM).
28385239	8	63	theme	water	1249:1253	arg1	rate					1274:1277	The water vapor transmission rate	1245:1277	The water vapor transmission rate	1245:1277	The water vapor transmission rate was determined and exhibited suitable for ideal wound dressings.
25439862	5	0	theme	ternary	758:764	arg1	membrane					776:783	The ternary composite membrane	754:783	The ternary composite membrane	754:783	The ternary composite membrane exhibits good water absorption ability with rapid uptake rate.
25439862	4	1	theme	water	672:676	arg1	capability					689:698	water absorption capability	672:698	water absorption capability	672:698	The morphology, structure, water solubility, water absorption capability and thermal properties of the NFs were characterized.
25439862	6	2	theme	wound	1153:1157	arg1	healing					1159:1165	intricate wound healing	1143:1165	intricate wound healing	1143:1165	This novel membrane favors fibroblast cell attachment and growth by providing a 3D environment which mimics the extracellular matrix (ECM) in skin and allows cells to move through the fibrous structure resulting in interlayer growth throughout the membrane, thus favoring potential for deep and intricate wound healing.
25439862	0	3	theme	potential	92:100	arg1	applications					102:113	potential applications	92:113	potential applications as wound dressing	92:131	Development of tannic acid/chitosan/pullulan composite nanofibers from aqueous solution for potential applications as wound dressing.
25439862	1	4	theme	Escherichia	345:355	arg1	bacteria					336:343	the Gram-negative bacteria	318:343	the Gram-negative bacteria Escherichia coli	318:360	This study presents the successful development of biocompatible tannic acid (TA)/chitosan (CS)/pullulan (PL) composite nanofibers (NFs) with synergistic antibacterial activity against the Gram-negative bacteria Escherichia coli.
25439862	1	5	theme	biocompatible	184:196	arg1	PL					239:240	PL	239:240	PL	239:240	This study presents the successful development of biocompatible tannic acid (TA)/chitosan (CS)/pullulan (PL) composite nanofibers (NFs) with synergistic antibacterial activity against the Gram-negative bacteria Escherichia coli.
25439862	1	5	theme	biocompatible	184:196	arg1	/pullulan					228:236	biocompatible tannic acid (TA)/chitosan (CS)/pullulan	184:236	biocompatible tannic acid (TA)/chitosan (CS)/pullulan (PL) composite nanofibers (NFs) with synergistic antibacterial activity against the Gram-negative bacteria Escherichia coli	184:360	This study presents the successful development of biocompatible tannic acid (TA)/chitosan (CS)/pullulan (PL) composite nanofibers (NFs) with synergistic antibacterial activity against the Gram-negative bacteria Escherichia coli.
25439862	1	6	theme	composite	243:251	arg1	NFs					265:267	NFs	265:267	NFs	265:267	This study presents the successful development of biocompatible tannic acid (TA)/chitosan (CS)/pullulan (PL) composite nanofibers (NFs) with synergistic antibacterial activity against the Gram-negative bacteria Escherichia coli.
25439862	1	6	theme	composite	243:251	arg1	nanofibers					253:262	biocompatible tannic acid (TA)/chitosan (CS)/pullulan (PL) composite nanofibers	184:262	biocompatible tannic acid (TA)/chitosan (CS)/pullulan (PL) composite nanofibers (NFs) with synergistic antibacterial activity against the Gram-negative bacteria Escherichia coli	184:360	This study presents the successful development of biocompatible tannic acid (TA)/chitosan (CS)/pullulan (PL) composite nanofibers (NFs) with synergistic antibacterial activity against the Gram-negative bacteria Escherichia coli.
25439862	0	7	theme	wound	118:122	arg1	dressing					124:131	wound dressing	118:131	wound dressing	118:131	Development of tannic acid/chitosan/pullulan composite nanofibers from aqueous solution for potential applications as wound dressing.
25439862	3	8	theme	nanofibrous	519:529	arg1	membranes					531:539	The ternary nanofibrous membranes	507:539	The ternary nanofibrous membranes	507:539	The ternary nanofibrous membranes were crosslinked to become water stable for potential applications as wound dressing.
25439862	1	9	theme	nanofibers	253:262	arg1	development					169:179	the successful development	154:179	the successful development of biocompatible tannic acid (TA)/chitosan (CS)/pullulan (PL) composite nanofibers (NFs) with synergistic antibacterial activity against the Gram-negative bacteria Escherichia coli	154:360	This study presents the successful development of biocompatible tannic acid (TA)/chitosan (CS)/pullulan (PL) composite nanofibers (NFs) with synergistic antibacterial activity against the Gram-negative bacteria Escherichia coli.
25439862	2	10	theme	solvents	497:504	arg1	usage					474:478	the usage	470:478	the usage of toxic organic solvents	470:504	The NFs were developed utilizing the forcespinning(®) (FS) technique from CS-CA aqueous solutions to avoid the usage of toxic organic solvents.
25439862	2	11	theme	organic	489:495	arg1	solvents					497:504	toxic organic solvents	483:504	toxic organic solvents	483:504	The NFs were developed utilizing the forcespinning(®) (FS) technique from CS-CA aqueous solutions to avoid the usage of toxic organic solvents.
25439862	6	12	theme	fibrous	1032:1038	arg1	structure					1040:1048	the fibrous structure	1028:1048	the fibrous structure resulting in interlayer growth	1028:1079	This novel membrane favors fibroblast cell attachment and growth by providing a 3D environment which mimics the extracellular matrix (ECM) in skin and allows cells to move through the fibrous structure resulting in interlayer growth throughout the membrane, thus favoring potential for deep and intricate wound healing.
25439862	1	13	theme	tannic	198:203	arg1	PL					239:240	PL	239:240	PL	239:240	This study presents the successful development of biocompatible tannic acid (TA)/chitosan (CS)/pullulan (PL) composite nanofibers (NFs) with synergistic antibacterial activity against the Gram-negative bacteria Escherichia coli.
25439862	1	13	theme	tannic	198:203	arg1	/pullulan					228:236	biocompatible tannic acid (TA)/chitosan (CS)/pullulan	184:236	biocompatible tannic acid (TA)/chitosan (CS)/pullulan (PL) composite nanofibers (NFs) with synergistic antibacterial activity against the Gram-negative bacteria Escherichia coli	184:360	This study presents the successful development of biocompatible tannic acid (TA)/chitosan (CS)/pullulan (PL) composite nanofibers (NFs) with synergistic antibacterial activity against the Gram-negative bacteria Escherichia coli.
25439862	2	14	theme	toxic	483:487	arg1	solvents					497:504	toxic organic solvents	483:504	toxic organic solvents	483:504	The NFs were developed utilizing the forcespinning(®) (FS) technique from CS-CA aqueous solutions to avoid the usage of toxic organic solvents.
25439862	3	15	theme	ternary	511:517	arg1	membranes					531:539	The ternary nanofibrous membranes	507:539	The ternary nanofibrous membranes	507:539	The ternary nanofibrous membranes were crosslinked to become water stable for potential applications as wound dressing.
25439862	1	16	theme	acid	205:208	arg1	PL					239:240	PL	239:240	PL	239:240	This study presents the successful development of biocompatible tannic acid (TA)/chitosan (CS)/pullulan (PL) composite nanofibers (NFs) with synergistic antibacterial activity against the Gram-negative bacteria Escherichia coli.
25439862	1	16	theme	acid	205:208	arg1	/pullulan					228:236	biocompatible tannic acid (TA)/chitosan (CS)/pullulan	184:236	biocompatible tannic acid (TA)/chitosan (CS)/pullulan (PL) composite nanofibers (NFs) with synergistic antibacterial activity against the Gram-negative bacteria Escherichia coli	184:360	This study presents the successful development of biocompatible tannic acid (TA)/chitosan (CS)/pullulan (PL) composite nanofibers (NFs) with synergistic antibacterial activity against the Gram-negative bacteria Escherichia coli.
25439862	5	17	theme	absorption	805:814	arg1	ability					816:822	good water absorption ability	794:822	good water absorption ability	794:822	The ternary composite membrane exhibits good water absorption ability with rapid uptake rate.
25439862	2	18	from	solutions	451:459	arg1	technique					422:430	the forcespinning(®) (FS) technique	396:430	the forcespinning(®) (FS) technique from CS-CA aqueous solutions to avoid the usage of toxic organic solvents	396:504	The NFs were developed utilizing the forcespinning(®) (FS) technique from CS-CA aqueous solutions to avoid the usage of toxic organic solvents.
25439862	4	19	theme	NFs	730:732	arg1	morphology					631:640	morphology	631:640	morphology	631:640	The morphology, structure, water solubility, water absorption capability and thermal properties of the NFs were characterized.
25439862	4	19	theme	NFs	730:732	arg1	capability					689:698	water absorption capability	672:698	water absorption capability	672:698	The morphology, structure, water solubility, water absorption capability and thermal properties of the NFs were characterized.
25439862	4	19	theme	NFs	730:732	arg1	properties					712:721	thermal properties	704:721	thermal properties	704:721	The morphology, structure, water solubility, water absorption capability and thermal properties of the NFs were characterized.
25439862	4	19	theme	NFs	730:732	arg1	structure					643:651	structure	643:651	structure	643:651	The morphology, structure, water solubility, water absorption capability and thermal properties of the NFs were characterized.
25439862	4	19	theme	NFs	730:732	arg1	solubility					660:669	water solubility	654:669	water solubility	654:669	The morphology, structure, water solubility, water absorption capability and thermal properties of the NFs were characterized.
25439862	1	20	dep	Escherichia	345:355	arg1	coli					357:360	coli	357:360	coli	357:360	This study presents the successful development of biocompatible tannic acid (TA)/chitosan (CS)/pullulan (PL) composite nanofibers (NFs) with synergistic antibacterial activity against the Gram-negative bacteria Escherichia coli.
25439862	6	21	theme	intricate	1143:1151	arg1	healing					1159:1165	intricate wound healing	1143:1165	intricate wound healing	1143:1165	This novel membrane favors fibroblast cell attachment and growth by providing a 3D environment which mimics the extracellular matrix (ECM) in skin and allows cells to move through the fibrous structure resulting in interlayer growth throughout the membrane, thus favoring potential for deep and intricate wound healing.
25439862	1	22	theme	TA	211:212	arg1	PL					239:240	PL	239:240	PL	239:240	This study presents the successful development of biocompatible tannic acid (TA)/chitosan (CS)/pullulan (PL) composite nanofibers (NFs) with synergistic antibacterial activity against the Gram-negative bacteria Escherichia coli.
25439862	1	22	theme	TA	211:212	arg1	/pullulan					228:236	biocompatible tannic acid (TA)/chitosan (CS)/pullulan	184:236	biocompatible tannic acid (TA)/chitosan (CS)/pullulan (PL) composite nanofibers (NFs) with synergistic antibacterial activity against the Gram-negative bacteria Escherichia coli	184:360	This study presents the successful development of biocompatible tannic acid (TA)/chitosan (CS)/pullulan (PL) composite nanofibers (NFs) with synergistic antibacterial activity against the Gram-negative bacteria Escherichia coli.
25439862	0	23	theme	acid/chitosan/pullulan	22:43	arg1	composite					45:53	tannic acid/chitosan/pullulan composite	15:53	tannic acid/chitosan/pullulan composite	15:53	Development of tannic acid/chitosan/pullulan composite nanofibers from aqueous solution for potential applications as wound dressing.
25439862	6	24	from	matrix	974:979	arg1	skin					990:993	skin	990:993	skin	990:993	This novel membrane favors fibroblast cell attachment and growth by providing a 3D environment which mimics the extracellular matrix (ECM) in skin and allows cells to move through the fibrous structure resulting in interlayer growth throughout the membrane, thus favoring potential for deep and intricate wound healing.
25439862	5	25	theme	good	794:797	arg1	ability					816:822	good water absorption ability	794:822	good water absorption ability	794:822	The ternary composite membrane exhibits good water absorption ability with rapid uptake rate.
25439862	5	26	theme	uptake	835:840	arg1	rate					842:845	rapid uptake rate	829:845	rapid uptake rate	829:845	The ternary composite membrane exhibits good water absorption ability with rapid uptake rate.
25439862	0	27	theme	tannic	15:20	arg1	composite					45:53	tannic acid/chitosan/pullulan composite	15:53	tannic acid/chitosan/pullulan composite	15:53	Development of tannic acid/chitosan/pullulan composite nanofibers from aqueous solution for potential applications as wound dressing.
25439862	6	28	theme	fibroblast	875:884	arg1	cell					886:889	fibroblast cell attachment and growth	875:911	cell	886:889	This novel membrane favors fibroblast cell attachment and growth by providing a 3D environment which mimics the extracellular matrix (ECM) in skin and allows cells to move through the fibrous structure resulting in interlayer growth throughout the membrane, thus favoring potential for deep and intricate wound healing.
25439862	4	29	theme	absorption	678:687	arg1	capability					689:698	water absorption capability	672:698	water absorption capability	672:698	The morphology, structure, water solubility, water absorption capability and thermal properties of the NFs were characterized.
25439862	1	30	theme	/chitosan	214:222	arg1	PL					239:240	PL	239:240	PL	239:240	This study presents the successful development of biocompatible tannic acid (TA)/chitosan (CS)/pullulan (PL) composite nanofibers (NFs) with synergistic antibacterial activity against the Gram-negative bacteria Escherichia coli.
25439862	1	30	theme	/chitosan	214:222	arg1	/pullulan					228:236	biocompatible tannic acid (TA)/chitosan (CS)/pullulan	184:236	biocompatible tannic acid (TA)/chitosan (CS)/pullulan (PL) composite nanofibers (NFs) with synergistic antibacterial activity against the Gram-negative bacteria Escherichia coli	184:360	This study presents the successful development of biocompatible tannic acid (TA)/chitosan (CS)/pullulan (PL) composite nanofibers (NFs) with synergistic antibacterial activity against the Gram-negative bacteria Escherichia coli.
25439862	1	31	theme	synergistic	275:285	arg1	activity					301:308	synergistic antibacterial activity	275:308	synergistic antibacterial activity against the Gram-negative bacteria Escherichia coli	275:360	This study presents the successful development of biocompatible tannic acid (TA)/chitosan (CS)/pullulan (PL) composite nanofibers (NFs) with synergistic antibacterial activity against the Gram-negative bacteria Escherichia coli.
25439862	6	32	theme	extracellular	960:972	arg1	matrix					974:979	the extracellular matrix	956:979	the extracellular matrix (ECM) in skin	956:993	This novel membrane favors fibroblast cell attachment and growth by providing a 3D environment which mimics the extracellular matrix (ECM) in skin and allows cells to move through the fibrous structure resulting in interlayer growth throughout the membrane, thus favoring potential for deep and intricate wound healing.
25439862	6	32	theme	extracellular	960:972	arg1	ECM					982:984	ECM	982:984	ECM	982:984	This novel membrane favors fibroblast cell attachment and growth by providing a 3D environment which mimics the extracellular matrix (ECM) in skin and allows cells to move through the fibrous structure resulting in interlayer growth throughout the membrane, thus favoring potential for deep and intricate wound healing.
25439862	4	33	theme	water	654:658	arg1	solubility					660:669	water solubility	654:669	water solubility	654:669	The morphology, structure, water solubility, water absorption capability and thermal properties of the NFs were characterized.
25439862	0	34	theme	composite	45:53	arg1	Development					0:10	Development	0:10	Development of tannic acid/chitosan/pullulan composite	0:53	Development of tannic acid/chitosan/pullulan composite nanofibers from aqueous solution for potential applications as wound dressing.
25439862	1	35	theme	antibacterial	287:299	arg1	activity					301:308	synergistic antibacterial activity	275:308	synergistic antibacterial activity against the Gram-negative bacteria Escherichia coli	275:360	This study presents the successful development of biocompatible tannic acid (TA)/chitosan (CS)/pullulan (PL) composite nanofibers (NFs) with synergistic antibacterial activity against the Gram-negative bacteria Escherichia coli.
25439862	5	36	theme	composite	766:774	arg1	membrane					776:783	The ternary composite membrane	754:783	The ternary composite membrane	754:783	The ternary composite membrane exhibits good water absorption ability with rapid uptake rate.
25439862	3	37	theme	potential	585:593	arg1	applications					595:606	potential applications	585:606	potential applications as wound dressing	585:624	The ternary nanofibrous membranes were crosslinked to become water stable for potential applications as wound dressing.
25439862	1	38	with	nanofibers	253:262	arg1	activity					301:308	synergistic antibacterial activity	275:308	synergistic antibacterial activity against the Gram-negative bacteria Escherichia coli	275:360	This study presents the successful development of biocompatible tannic acid (TA)/chitosan (CS)/pullulan (PL) composite nanofibers (NFs) with synergistic antibacterial activity against the Gram-negative bacteria Escherichia coli.
25439862	1	39	theme	CS	225:226	arg1	PL					239:240	PL	239:240	PL	239:240	This study presents the successful development of biocompatible tannic acid (TA)/chitosan (CS)/pullulan (PL) composite nanofibers (NFs) with synergistic antibacterial activity against the Gram-negative bacteria Escherichia coli.
25439862	1	39	theme	CS	225:226	arg1	/pullulan					228:236	biocompatible tannic acid (TA)/chitosan (CS)/pullulan	184:236	biocompatible tannic acid (TA)/chitosan (CS)/pullulan (PL) composite nanofibers (NFs) with synergistic antibacterial activity against the Gram-negative bacteria Escherichia coli	184:360	This study presents the successful development of biocompatible tannic acid (TA)/chitosan (CS)/pullulan (PL) composite nanofibers (NFs) with synergistic antibacterial activity against the Gram-negative bacteria Escherichia coli.
25439862	0	40	theme	aqueous	71:77	arg1	solution					79:86	aqueous solution	71:86	aqueous solution for potential applications as wound dressing	71:131	Development of tannic acid/chitosan/pullulan composite nanofibers from aqueous solution for potential applications as wound dressing.
25439862	5	41	theme	water	799:803	arg1	ability					816:822	good water absorption ability	794:822	good water absorption ability	794:822	The ternary composite membrane exhibits good water absorption ability with rapid uptake rate.
25439862	6	42	theme	novel	853:857	arg1	membrane					859:866	This novel membrane	848:866	This novel membrane	848:866	This novel membrane favors fibroblast cell attachment and growth by providing a 3D environment which mimics the extracellular matrix (ECM) in skin and allows cells to move through the fibrous structure resulting in interlayer growth throughout the membrane, thus favoring potential for deep and intricate wound healing.
25439862	4	43	theme	thermal	704:710	arg1	properties					712:721	thermal properties	704:721	thermal properties	704:721	The morphology, structure, water solubility, water absorption capability and thermal properties of the NFs were characterized.
25439862	2	44	theme	CS-CA	437:441	arg1	solutions					451:459	CS-CA aqueous solutions	437:459	CS-CA aqueous solutions	437:459	The NFs were developed utilizing the forcespinning(®) (FS) technique from CS-CA aqueous solutions to avoid the usage of toxic organic solvents.
25439862	2	45	theme	forcespinning	400:412	arg1	technique					422:430	the forcespinning(®) (FS) technique	396:430	the forcespinning(®) (FS) technique from CS-CA aqueous solutions to avoid the usage of toxic organic solvents	396:504	The NFs were developed utilizing the forcespinning(®) (FS) technique from CS-CA aqueous solutions to avoid the usage of toxic organic solvents.
25439862	6	46	theme	3D	928:929	arg1	environment					931:941	a 3D environment	926:941	a 3D environment which mimics the extracellular matrix (ECM) in skin and allows cells to move through the fibrous structure resulting in interlayer growth throughout the membrane, thus favoring potential for deep and intricate wound healing	926:1165	This novel membrane favors fibroblast cell attachment and growth by providing a 3D environment which mimics the extracellular matrix (ECM) in skin and allows cells to move through the fibrous structure resulting in interlayer growth throughout the membrane, thus favoring potential for deep and intricate wound healing.
25439862	6	47	dep	cell	886:889	arg1	attachment					891:900	attachment	891:900	attachment	891:900	This novel membrane favors fibroblast cell attachment and growth by providing a 3D environment which mimics the extracellular matrix (ECM) in skin and allows cells to move through the fibrous structure resulting in interlayer growth throughout the membrane, thus favoring potential for deep and intricate wound healing.
25439862	3	48	theme	wound	611:615	arg1	dressing					617:624	wound dressing	611:624	wound dressing	611:624	The ternary nanofibrous membranes were crosslinked to become water stable for potential applications as wound dressing.
25439862	4	49	dep	morphology	631:640	arg1	The					627:629	The	627:629	The	627:629	The morphology, structure, water solubility, water absorption capability and thermal properties of the NFs were characterized.
25439862	1	50	theme	/pullulan	228:236	arg1	NFs					265:267	NFs	265:267	NFs	265:267	This study presents the successful development of biocompatible tannic acid (TA)/chitosan (CS)/pullulan (PL) composite nanofibers (NFs) with synergistic antibacterial activity against the Gram-negative bacteria Escherichia coli.
25439862	1	50	theme	/pullulan	228:236	arg1	nanofibers					253:262	biocompatible tannic acid (TA)/chitosan (CS)/pullulan (PL) composite nanofibers	184:262	biocompatible tannic acid (TA)/chitosan (CS)/pullulan (PL) composite nanofibers (NFs) with synergistic antibacterial activity against the Gram-negative bacteria Escherichia coli	184:360	This study presents the successful development of biocompatible tannic acid (TA)/chitosan (CS)/pullulan (PL) composite nanofibers (NFs) with synergistic antibacterial activity against the Gram-negative bacteria Escherichia coli.
25439862	2	51	dep	forcespinning	400:412	arg1	®					414:414	®	414:414	®	414:414	The NFs were developed utilizing the forcespinning(®) (FS) technique from CS-CA aqueous solutions to avoid the usage of toxic organic solvents.
25439862	2	51	dep	forcespinning	400:412	arg1	FS					418:419	FS	418:419	FS	418:419	The NFs were developed utilizing the forcespinning(®) (FS) technique from CS-CA aqueous solutions to avoid the usage of toxic organic solvents.
25439862	1	52	theme	successful	158:167	arg1	development					169:179	the successful development	154:179	the successful development of biocompatible tannic acid (TA)/chitosan (CS)/pullulan (PL) composite nanofibers (NFs) with synergistic antibacterial activity against the Gram-negative bacteria Escherichia coli	154:360	This study presents the successful development of biocompatible tannic acid (TA)/chitosan (CS)/pullulan (PL) composite nanofibers (NFs) with synergistic antibacterial activity against the Gram-negative bacteria Escherichia coli.
25439862	6	53	theme	interlayer	1063:1072	arg1	growth					1074:1079	interlayer growth	1063:1079	interlayer growth	1063:1079	This novel membrane favors fibroblast cell attachment and growth by providing a 3D environment which mimics the extracellular matrix (ECM) in skin and allows cells to move through the fibrous structure resulting in interlayer growth throughout the membrane, thus favoring potential for deep and intricate wound healing.
25439862	2	54	theme	aqueous	443:449	arg1	solutions					451:459	CS-CA aqueous solutions	437:459	CS-CA aqueous solutions	437:459	The NFs were developed utilizing the forcespinning(®) (FS) technique from CS-CA aqueous solutions to avoid the usage of toxic organic solvents.
25439862	1	55	theme	Gram-negative	322:334	arg1	bacteria					336:343	the Gram-negative bacteria	318:343	the Gram-negative bacteria Escherichia coli	318:360	This study presents the successful development of biocompatible tannic acid (TA)/chitosan (CS)/pullulan (PL) composite nanofibers (NFs) with synergistic antibacterial activity against the Gram-negative bacteria Escherichia coli.
25439862	5	56	theme	rapid	829:833	arg1	rate					842:845	rapid uptake rate	829:845	rapid uptake rate	829:845	The ternary composite membrane exhibits good water absorption ability with rapid uptake rate.
25542105	5	0	dep	Fourier	797:803	arg1	transform					805:813	transform	805:813	transform infrared spectrum, monosaccharide composition, and antioxidant activity	805:885	The oligosaccharides were partially characterized by Fourier transform infrared spectrum, monosaccharide composition, and antioxidant activity.
25542105	0	1	theme	antioxidant	80:90	arg1	activities					92:101	their antioxidant activities	74:101	their antioxidant activities	74:101	Preparation of the oligosaccharides derived from Flammulina velutipes and their antioxidant activities.
25542105	5	2	dep	transform	805:813	arg1	infrared					815:822	infrared	815:822	transform infrared spectrum, monosaccharide composition, and antioxidant activity	805:885	The oligosaccharides were partially characterized by Fourier transform infrared spectrum, monosaccharide composition, and antioxidant activity.
25542105	1	3	theme	F.	182:183	arg1	polysaccharides					195:209	F. velutipes polysaccharides	182:209	F. velutipes polysaccharides with hydrogen peroxide (H2O2)	182:239	The oligosaccharides were prepared from Flammulina velutipes by hydrolysis of F. velutipes polysaccharides with hydrogen peroxide (H2O2).
25542105	6	4	theme	reducing	1005:1012	arg1	capacity					1014:1021	reducing capacity	1005:1021	reducing capacity	1005:1021	The results indicate that the oligosaccharides derived from F. velutipes showed strong hydroxyl radical activity and reducing capacity at the concentration of 100 μg/mL.
25542105	4	5	theme	extraction	725:734	arg1	method					736:741	hot water extraction method	715:741	hot water extraction method	715:741	Under these optimum conditions, the maximal yield of the oligosaccharides reached 17.10%, which was higher than that of hot water extraction method.
25542105	4	6	theme	water	719:723	arg1	method					736:741	hot water extraction method	715:741	hot water extraction method	715:741	Under these optimum conditions, the maximal yield of the oligosaccharides reached 17.10%, which was higher than that of hot water extraction method.
25542105	3	7	dep	temperature	405:415	arg1	i.e.					391:394	i.e.	391:394	i.e.	391:394	FVOs yields were affected by three factors, i.e. reaction temperature, H2O2 concentration, and time, which were optimized by using an orthogonal design experiments as follows: reaction temperature 70°C, H2O2 concentration 3%, and reaction time 6h.
25542105	3	8	theme	reaction	523:530	arg1	70°C					544:547	reaction temperature 70°C	523:547	reaction temperature 70°C	523:547	FVOs yields were affected by three factors, i.e. reaction temperature, H2O2 concentration, and time, which were optimized by using an orthogonal design experiments as follows: reaction temperature 70°C, H2O2 concentration 3%, and reaction time 6h.
25542105	1	9	theme	velutipes	185:193	arg1	polysaccharides					195:209	F. velutipes polysaccharides	182:209	F. velutipes polysaccharides with hydrogen peroxide (H2O2)	182:239	The oligosaccharides were prepared from Flammulina velutipes by hydrolysis of F. velutipes polysaccharides with hydrogen peroxide (H2O2).
25542105	3	10	theme	temperature	532:542	arg1	70°C					544:547	reaction temperature 70°C	523:547	reaction temperature 70°C	523:547	FVOs yields were affected by three factors, i.e. reaction temperature, H2O2 concentration, and time, which were optimized by using an orthogonal design experiments as follows: reaction temperature 70°C, H2O2 concentration 3%, and reaction time 6h.
25542105	1	11	theme	polysaccharides	195:209	arg1	hydrolysis					168:177	hydrolysis	168:177	hydrolysis of F. velutipes polysaccharides with hydrogen peroxide (H2O2)	168:239	The oligosaccharides were prepared from Flammulina velutipes by hydrolysis of F. velutipes polysaccharides with hydrogen peroxide (H2O2).
25542105	2	12	theme	hydrolysis	327:336	arg1	process					338:344	the hydrolysis process	323:344	the hydrolysis process	323:344	The yields of F. velutipes derived oligosaccharides (FVOs) were monitored during the hydrolysis process.
25542105	3	13	theme	reaction	396:403	arg1	temperature					405:415	reaction temperature	396:415	reaction temperature	396:415	FVOs yields were affected by three factors, i.e. reaction temperature, H2O2 concentration, and time, which were optimized by using an orthogonal design experiments as follows: reaction temperature 70°C, H2O2 concentration 3%, and reaction time 6h.
25542105	6	14	theme	radical	984:990	arg1	activity					992:999	strong hydroxyl radical activity	968:999	strong hydroxyl radical activity	968:999	The results indicate that the oligosaccharides derived from F. velutipes showed strong hydroxyl radical activity and reducing capacity at the concentration of 100 μg/mL.
25542105	1	15	theme	hydrogen	216:223	arg1	H2O2					235:238	H2O2	235:238	H2O2	235:238	The oligosaccharides were prepared from Flammulina velutipes by hydrolysis of F. velutipes polysaccharides with hydrogen peroxide (H2O2).
25542105	1	15	theme	hydrogen	216:223	arg1	peroxide					225:232	hydrogen peroxide	216:232	hydrogen peroxide (H2O2)	216:239	The oligosaccharides were prepared from Flammulina velutipes by hydrolysis of F. velutipes polysaccharides with hydrogen peroxide (H2O2).
25542105	0	16	theme	oligosaccharides	19:34	arg1	Preparation					0:10	Preparation	0:10	Preparation of the oligosaccharides	0:34	Preparation of the oligosaccharides derived from Flammulina velutipes and their antioxidant activities.
25542105	6	17	theme	hydroxyl	975:982	arg1	activity					992:999	strong hydroxyl radical activity	968:999	strong hydroxyl radical activity	968:999	The results indicate that the oligosaccharides derived from F. velutipes showed strong hydroxyl radical activity and reducing capacity at the concentration of 100 μg/mL.
25542105	3	18	theme	H2O2	550:553	arg1	%					570:570	H2O2 concentration 3%	550:570	H2O2 concentration 3%	550:570	FVOs yields were affected by three factors, i.e. reaction temperature, H2O2 concentration, and time, which were optimized by using an orthogonal design experiments as follows: reaction temperature 70°C, H2O2 concentration 3%, and reaction time 6h.
25542105	6	19	theme	strong	968:973	arg1	activity					992:999	strong hydroxyl radical activity	968:999	strong hydroxyl radical activity	968:999	The results indicate that the oligosaccharides derived from F. velutipes showed strong hydroxyl radical activity and reducing capacity at the concentration of 100 μg/mL.
25542105	3	20	theme	concentration	555:567	arg1	%					570:570	H2O2 concentration 3%	550:570	H2O2 concentration 3%	550:570	FVOs yields were affected by three factors, i.e. reaction temperature, H2O2 concentration, and time, which were optimized by using an orthogonal design experiments as follows: reaction temperature 70°C, H2O2 concentration 3%, and reaction time 6h.
25542105	4	21	theme	optimum	607:613	arg1	conditions					615:624	these optimum conditions	601:624	these optimum conditions	601:624	Under these optimum conditions, the maximal yield of the oligosaccharides reached 17.10%, which was higher than that of hot water extraction method.
25542105	3	22	theme	H2O2	418:421	arg1	concentration					423:435	H2O2 concentration	418:435	H2O2 concentration	418:435	FVOs yields were affected by three factors, i.e. reaction temperature, H2O2 concentration, and time, which were optimized by using an orthogonal design experiments as follows: reaction temperature 70°C, H2O2 concentration 3%, and reaction time 6h.
25542105	3	23	theme	reaction	577:584	arg1	6h					591:592	reaction time 6h	577:592	reaction time 6h	577:592	FVOs yields were affected by three factors, i.e. reaction temperature, H2O2 concentration, and time, which were optimized by using an orthogonal design experiments as follows: reaction temperature 70°C, H2O2 concentration 3%, and reaction time 6h.
25542105	5	24	theme	antioxidant	866:876	arg1	activity					878:885	antioxidant activity	866:885	antioxidant activity	866:885	The oligosaccharides were partially characterized by Fourier transform infrared spectrum, monosaccharide composition, and antioxidant activity.
25542105	6	25	theme	μg/mL	1051:1055	arg1	concentration					1030:1042	the concentration	1026:1042	the concentration of 100 μg/mL	1026:1055	The results indicate that the oligosaccharides derived from F. velutipes showed strong hydroxyl radical activity and reducing capacity at the concentration of 100 μg/mL.
25542105	3	26	theme	time	586:589	arg1	6h					591:592	reaction time 6h	577:592	reaction time 6h	577:592	FVOs yields were affected by three factors, i.e. reaction temperature, H2O2 concentration, and time, which were optimized by using an orthogonal design experiments as follows: reaction temperature 70°C, H2O2 concentration 3%, and reaction time 6h.
25542105	4	27	theme	hot	715:717	arg1	method					736:741	hot water extraction method	715:741	hot water extraction method	715:741	Under these optimum conditions, the maximal yield of the oligosaccharides reached 17.10%, which was higher than that of hot water extraction method.
25542105	6	28	theme	F.	948:949	arg1	velutipes					951:959	F. velutipes	948:959	F. velutipes	948:959	The results indicate that the oligosaccharides derived from F. velutipes showed strong hydroxyl radical activity and reducing capacity at the concentration of 100 μg/mL.
25542105	6	29	attach	derived	935:941	arg2	oligosaccharides					918:933	the oligosaccharides	914:933	the oligosaccharides derived from F. velutipes	914:959	The results indicate that the oligosaccharides derived from F. velutipes showed strong hydroxyl radical activity and reducing capacity at the concentration of 100 μg/mL.
25542105	6	29	attach	derived	935:941	arg1	velutipes					951:959	F. velutipes	948:959	F. velutipes	948:959	The results indicate that the oligosaccharides derived from F. velutipes showed strong hydroxyl radical activity and reducing capacity at the concentration of 100 μg/mL.
25542105	3	30	theme	orthogonal	481:490	arg1	design					492:497	an orthogonal design	478:497	an orthogonal design experiments	478:509	FVOs yields were affected by three factors, i.e. reaction temperature, H2O2 concentration, and time, which were optimized by using an orthogonal design experiments as follows: reaction temperature 70°C, H2O2 concentration 3%, and reaction time 6h.
25542105	0	31	theme	Flammulina	49:58	arg1	velutipes					60:68	Flammulina velutipes	49:68	Flammulina velutipes	49:68	Preparation of the oligosaccharides derived from Flammulina velutipes and their antioxidant activities.
25542105	3	32	theme	design	492:497	arg1	experiments					499:509	an orthogonal design experiments	478:509	an orthogonal design experiments	478:509	FVOs yields were affected by three factors, i.e. reaction temperature, H2O2 concentration, and time, which were optimized by using an orthogonal design experiments as follows: reaction temperature 70°C, H2O2 concentration 3%, and reaction time 6h.
25542105	1	33	theme	Flammulina	144:153	arg1	velutipes					155:163	Flammulina velutipes	144:163	Flammulina velutipes	144:163	The oligosaccharides were prepared from Flammulina velutipes by hydrolysis of F. velutipes polysaccharides with hydrogen peroxide (H2O2).
25542105	3	34	dep	follows	514:520	arg1	70°C					544:547	reaction temperature 70°C	523:547	reaction temperature 70°C	523:547	FVOs yields were affected by three factors, i.e. reaction temperature, H2O2 concentration, and time, which were optimized by using an orthogonal design experiments as follows: reaction temperature 70°C, H2O2 concentration 3%, and reaction time 6h.
25542105	3	34	dep	follows	514:520	arg1	6h					591:592	reaction time 6h	577:592	reaction time 6h	577:592	FVOs yields were affected by three factors, i.e. reaction temperature, H2O2 concentration, and time, which were optimized by using an orthogonal design experiments as follows: reaction temperature 70°C, H2O2 concentration 3%, and reaction time 6h.
25542105	3	34	dep	follows	514:520	arg1	%					570:570	H2O2 concentration 3%	550:570	H2O2 concentration 3%	550:570	FVOs yields were affected by three factors, i.e. reaction temperature, H2O2 concentration, and time, which were optimized by using an orthogonal design experiments as follows: reaction temperature 70°C, H2O2 concentration 3%, and reaction time 6h.
25542105	1	35	with	polysaccharides	195:209	arg1	H2O2					235:238	H2O2	235:238	H2O2	235:238	The oligosaccharides were prepared from Flammulina velutipes by hydrolysis of F. velutipes polysaccharides with hydrogen peroxide (H2O2).
25542105	1	35	with	polysaccharides	195:209	arg1	peroxide					225:232	hydrogen peroxide	216:232	hydrogen peroxide (H2O2)	216:239	The oligosaccharides were prepared from Flammulina velutipes by hydrolysis of F. velutipes polysaccharides with hydrogen peroxide (H2O2).
25542105	3	36	theme	FVOs	347:350	arg1	yields					352:357	FVOs yields	347:357	FVOs yields	347:357	FVOs yields were affected by three factors, i.e. reaction temperature, H2O2 concentration, and time, which were optimized by using an orthogonal design experiments as follows: reaction temperature 70°C, H2O2 concentration 3%, and reaction time 6h.
25542105	5	37	theme	monosaccharide	834:847	arg1	composition					849:859	monosaccharide composition	834:859	monosaccharide composition	834:859	The oligosaccharides were partially characterized by Fourier transform infrared spectrum, monosaccharide composition, and antioxidant activity.
25542105	2	38	theme	velutipes	259:267	arg1	yields					246:251	The yields	242:251	The yields of F. velutipes derived oligosaccharides (FVOs)	242:299	The yields of F. velutipes derived oligosaccharides (FVOs) were monitored during the hydrolysis process.
25542105	4	39	theme	maximal	631:637	arg1	yield					639:643	the maximal yield	627:643	the maximal yield of the oligosaccharides	627:667	Under these optimum conditions, the maximal yield of the oligosaccharides reached 17.10%, which was higher than that of hot water extraction method.
25542105	2	40	theme	F.	256:257	arg1	velutipes					259:267	F. velutipes	256:267	F. velutipes derived oligosaccharides (FVOs)	256:299	The yields of F. velutipes derived oligosaccharides (FVOs) were monitored during the hydrolysis process.
25542105	4	41	theme	oligosaccharides	652:667	arg1	yield					639:643	the maximal yield	627:643	the maximal yield of the oligosaccharides	627:667	Under these optimum conditions, the maximal yield of the oligosaccharides reached 17.10%, which was higher than that of hot water extraction method.
27630120	11	0	theme	concomitant	1768:1778	arg1	mechanisms					1780:1789	concomitant mechanisms	1768:1789	concomitant mechanisms involved in cell wall assembly/disassembly during fruit development and storage	1768:1869	Moreover, new insights into the potential role of peroxidases and transporters, along with cell wall metabolism open the way to further studies on concomitant mechanisms involved in cell wall assembly/disassembly during fruit development and storage.
27630120	11	1	theme	transporters	1687:1698	arg1	role					1663:1666	the potential role	1649:1666	the potential role of peroxidases and transporters	1649:1698	Moreover, new insights into the potential role of peroxidases and transporters, along with cell wall metabolism open the way to further studies on concomitant mechanisms involved in cell wall assembly/disassembly during fruit development and storage.
27630120	6	2	theme	galactoglucomannan	989:1006	arg1	evolutions					975:984	Fine structural evolutions	959:984	Fine structural evolutions of galactoglucomannan	959:1006	Fine structural evolutions of galactoglucomannan were strongly correlated with mannan synthase, glucanase (GH9) and β-galactosidase gene expression.
27630120	5	3	with	related	865:871	arg1	target					933:938	a particular target	920:938	a particular target on hemicelluloses	920:956	The early development genes were mainly related to cell wall biosynthesis and degradation with a particular target on hemicelluloses.
27630120	4	4	theme	putative	649:656	arg1	enzymes					658:664	putative enzymes	649:664	putative enzymes	649:664	RESULTS Combined transcriptomic and biochemical analyses allowed the identification of putative enzymes and related annotated members of gene families involved in cell wall polysaccharide composition and structural changes during apple fruit growth and ripening.
27630120	11	5	theme	peroxidases	1671:1681	arg1	role					1663:1666	the potential role	1649:1666	the potential role of peroxidases and transporters	1649:1698	Moreover, new insights into the potential role of peroxidases and transporters, along with cell wall metabolism open the way to further studies on concomitant mechanisms involved in cell wall assembly/disassembly during fruit development and storage.
27630120	11	6	theme	cell	1803:1806	arg1	assembly/disassembly					1813:1832	cell wall assembly/disassembly	1803:1832	cell wall assembly/disassembly during fruit development and storage	1803:1869	Moreover, new insights into the potential role of peroxidases and transporters, along with cell wall metabolism open the way to further studies on concomitant mechanisms involved in cell wall assembly/disassembly during fruit development and storage.
27630120	11	7	theme	cell	1712:1715	arg1	metabolism					1722:1731	cell wall metabolism	1712:1731	cell wall metabolism	1712:1731	Moreover, new insights into the potential role of peroxidases and transporters, along with cell wall metabolism open the way to further studies on concomitant mechanisms involved in cell wall assembly/disassembly during fruit development and storage.
27630120	2	8	theme	wall	281:284	arg1	remodelling					301:311	Cell wall polysaccharide remodelling	276:311	Cell wall polysaccharide remodelling	276:311	Cell wall polysaccharide remodelling largely contributes to the elaboration of fleshy fruit texture.
27630120	1	9	theme	events	182:187	arg1	series					160:165	a series	158:165	a series of biochemical events that modify appearance, flavour and texture throughout fruit development and ripening	158:273	BACKGROUND Fruit quality depends on a series of biochemical events that modify appearance, flavour and texture throughout fruit development and ripening.
27630120	6	10	theme	Fine	959:962	arg1	evolutions					975:984	Fine structural evolutions	959:984	Fine structural evolutions of galactoglucomannan	959:1006	Fine structural evolutions of galactoglucomannan were strongly correlated with mannan synthase, glucanase (GH9) and β-galactosidase gene expression.
27630120	8	11	theme	fruit	1388:1392	arg1	development					1394:1404	early fruit development	1382:1404	early fruit development	1382:1404	CONCLUSIONS Hemicelluloses undergo major structural changes particularly during early fruit development.
27630120	7	12	theme	cell	1166:1169	arg1	expansion					1171:1179	cell expansion	1166:1179	cell expansion	1166:1179	In contrast, fewer genes related to pectin metabolism and cell expansion (expansin genes) were observed in ripening fruit combined with expected changes in cell wall polysaccharide composition.
27630120	4	13	theme	families	704:711	arg1	members					688:694	related annotated members	670:694	related annotated members of gene families involved in cell wall polysaccharide composition and structural changes during apple fruit growth and ripening	670:822	RESULTS Combined transcriptomic and biochemical analyses allowed the identification of putative enzymes and related annotated members of gene families involved in cell wall polysaccharide composition and structural changes during apple fruit growth and ripening.
27630120	4	13	theme	families	704:711	arg1	enzymes					658:664	putative enzymes	649:664	putative enzymes	649:664	RESULTS Combined transcriptomic and biochemical analyses allowed the identification of putative enzymes and related annotated members of gene families involved in cell wall polysaccharide composition and structural changes during apple fruit growth and ripening.
27630120	5	14	theme	particular	922:931	arg1	target					933:938	a particular target	920:938	a particular target on hemicelluloses	920:956	The early development genes were mainly related to cell wall biosynthesis and degradation with a particular target on hemicelluloses.
27630120	8	15	theme	major	1337:1341	arg1	changes					1354:1360	major structural changes	1337:1360	major structural changes	1337:1360	CONCLUSIONS Hemicelluloses undergo major structural changes particularly during early fruit development.
27630120	1	16	theme	Fruit	133:137	arg1	quality					139:145	BACKGROUND Fruit quality	122:145	BACKGROUND Fruit quality	122:145	BACKGROUND Fruit quality depends on a series of biochemical events that modify appearance, flavour and texture throughout fruit development and ripening.
27630120	4	17	theme	fruit	798:802	arg1	growth					804:809	apple fruit growth	792:809	apple fruit growth	792:809	RESULTS Combined transcriptomic and biochemical analyses allowed the identification of putative enzymes and related annotated members of gene families involved in cell wall polysaccharide composition and structural changes during apple fruit growth and ripening.
27630120	11	18	theme	new	1631:1633	arg1	insights					1635:1642	new insights	1631:1642	new insights into the potential role of peroxidases and transporters, along with cell wall metabolism	1631:1731	Moreover, new insights into the potential role of peroxidases and transporters, along with cell wall metabolism open the way to further studies on concomitant mechanisms involved in cell wall assembly/disassembly during fruit development and storage.
27630120	5	19	theme	early	829:833	arg1	genes					847:851	The early development genes	825:851	The early development genes	825:851	The early development genes were mainly related to cell wall biosynthesis and degradation with a particular target on hemicelluloses.
27630120	5	20	from	target	933:938	arg1	hemicelluloses					943:956	hemicelluloses	943:956	hemicelluloses	943:956	The early development genes were mainly related to cell wall biosynthesis and degradation with a particular target on hemicelluloses.
27630120	4	21	theme	structural	766:775	arg1	changes					777:783	structural changes	766:783	structural changes during apple fruit growth and ripening	766:822	RESULTS Combined transcriptomic and biochemical analyses allowed the identification of putative enzymes and related annotated members of gene families involved in cell wall polysaccharide composition and structural changes during apple fruit growth and ripening.
27630120	7	22	located	observed	1203:1210	arg2	genes					1191:1195	expansin genes	1182:1195	expansin genes	1182:1195	In contrast, fewer genes related to pectin metabolism and cell expansion (expansin genes) were observed in ripening fruit combined with expected changes in cell wall polysaccharide composition.
27630120	7	22	located	observed	1203:1210	arg1	contrast					1111:1118	contrast	1111:1118	contrast	1111:1118	In contrast, fewer genes related to pectin metabolism and cell expansion (expansin genes) were observed in ripening fruit combined with expected changes in cell wall polysaccharide composition.
27630120	7	22	located	observed	1203:1210	arg2	genes					1127:1131	fewer genes	1121:1131	fewer genes related to pectin metabolism and cell expansion (expansin genes)	1121:1196	In contrast, fewer genes related to pectin metabolism and cell expansion (expansin genes) were observed in ripening fruit combined with expected changes in cell wall polysaccharide composition.
27630120	6	23	theme	gene	1091:1094	arg1	expression					1096:1105	β-galactosidase gene expression	1075:1105	β-galactosidase gene expression	1075:1105	Fine structural evolutions of galactoglucomannan were strongly correlated with mannan synthase, glucanase (GH9) and β-galactosidase gene expression.
27630120	3	24	theme	several	386:392	arg1	genes					394:398	several genes	386:398	several genes	386:398	Although several genes and enzymes involved in cell wall polysaccharide biosynthesis and modifications are known, their coordinated activity in these processes is yet to be discovered.
27630120	11	25	theme	potential	1653:1661	arg1	role					1663:1666	the potential role	1649:1666	the potential role of peroxidases and transporters	1649:1698	Moreover, new insights into the potential role of peroxidases and transporters, along with cell wall metabolism open the way to further studies on concomitant mechanisms involved in cell wall assembly/disassembly during fruit development and storage.
27630120	0	26	theme	related	97:103	arg1	genes					115:119	related expressed genes	97:119	related expressed genes	97:119	Cell wall dynamics during apple development and storage involves hemicellulose modifications and related expressed genes.
27630120	9	27	theme	early	1426:1430	arg1	genes					1458:1462	early expressed β-galactosidase genes	1426:1462	early expressed β-galactosidase genes	1426:1462	The high number of early expressed β-galactosidase genes questions their function on galactosylated structures during fruit development and storage.
27630120	7	28	from	changes	1253:1259	arg1	composition					1289:1299	cell wall polysaccharide composition	1264:1299	cell wall polysaccharide composition	1264:1299	In contrast, fewer genes related to pectin metabolism and cell expansion (expansin genes) were observed in ripening fruit combined with expected changes in cell wall polysaccharide composition.
27630120	7	29	theme	polysaccharide	1274:1287	arg1	composition					1289:1299	cell wall polysaccharide composition	1264:1299	cell wall polysaccharide composition	1264:1299	In contrast, fewer genes related to pectin metabolism and cell expansion (expansin genes) were observed in ripening fruit combined with expected changes in cell wall polysaccharide composition.
27630120	9	30	theme	β-galactosidase	1442:1456	arg1	genes					1458:1462	early expressed β-galactosidase genes	1426:1462	early expressed β-galactosidase genes	1426:1462	The high number of early expressed β-galactosidase genes questions their function on galactosylated structures during fruit development and storage.
27630120	0	31	theme	Cell	0:3	arg1	dynamics					10:17	Cell wall dynamics	0:17	Cell wall dynamics during apple development and storage	0:54	Cell wall dynamics during apple development and storage involves hemicellulose modifications and related expressed genes.
27630120	3	32	theme	coordinated	497:507	arg1	activity					509:516	their coordinated activity	491:516	their coordinated activity in these processes	491:535	Although several genes and enzymes involved in cell wall polysaccharide biosynthesis and modifications are known, their coordinated activity in these processes is yet to be discovered.
27630120	3	33	theme	polysaccharide	434:447	arg1	biosynthesis					449:460	cell wall polysaccharide biosynthesis	424:460	cell wall polysaccharide biosynthesis	424:460	Although several genes and enzymes involved in cell wall polysaccharide biosynthesis and modifications are known, their coordinated activity in these processes is yet to be discovered.
27630120	7	34	theme	cell	1264:1267	arg1	composition					1289:1299	cell wall polysaccharide composition	1264:1299	cell wall polysaccharide composition	1264:1299	In contrast, fewer genes related to pectin metabolism and cell expansion (expansin genes) were observed in ripening fruit combined with expected changes in cell wall polysaccharide composition.
27630120	0	35	theme	apple	26:30	arg1	development					32:42	apple development	26:42	apple development	26:42	Cell wall dynamics during apple development and storage involves hemicellulose modifications and related expressed genes.
27630120	9	36	theme	galactosylated	1492:1505	arg1	structures					1507:1516	galactosylated structures	1492:1516	galactosylated structures	1492:1516	The high number of early expressed β-galactosidase genes questions their function on galactosylated structures during fruit development and storage.
27630120	3	37	theme	cell	424:427	arg1	biosynthesis					449:460	cell wall polysaccharide biosynthesis	424:460	cell wall polysaccharide biosynthesis	424:460	Although several genes and enzymes involved in cell wall polysaccharide biosynthesis and modifications are known, their coordinated activity in these processes is yet to be discovered.
27630120	2	38	theme	texture	368:374	arg1	elaboration					340:350	the elaboration	336:350	the elaboration of fleshy fruit texture	336:374	Cell wall polysaccharide remodelling largely contributes to the elaboration of fleshy fruit texture.
27630120	10	39	theme	cell	1575:1578	arg1	substrate					1585:1593	cell wall substrate	1575:1593	cell wall substrate	1575:1593	Their activity and cell wall substrate remains to be identified.
27630120	3	40	from	activity	509:516	arg1	processes					527:535	these processes	521:535	these processes	521:535	Although several genes and enzymes involved in cell wall polysaccharide biosynthesis and modifications are known, their coordinated activity in these processes is yet to be discovered.
27630120	2	41	theme	fleshy	355:360	arg1	texture					368:374	fleshy fruit texture	355:374	fleshy fruit texture	355:374	Cell wall polysaccharide remodelling largely contributes to the elaboration of fleshy fruit texture.
27630120	4	42	theme	enzymes	658:664	arg1	identification					631:644	the identification	627:644	the identification of putative enzymes and related annotated members of gene families involved in cell wall polysaccharide composition and structural changes during apple fruit growth and ripening	627:822	RESULTS Combined transcriptomic and biochemical analyses allowed the identification of putative enzymes and related annotated members of gene families involved in cell wall polysaccharide composition and structural changes during apple fruit growth and ripening.
27630120	1	43	theme	fruit	244:248	arg1	development					250:260	fruit development	244:260	fruit development	244:260	BACKGROUND Fruit quality depends on a series of biochemical events that modify appearance, flavour and texture throughout fruit development and ripening.
27630120	11	44	dep	along	1701:1705	arg1	with					1707:1710	with	1707:1710	with	1707:1710	Moreover, new insights into the potential role of peroxidases and transporters, along with cell wall metabolism open the way to further studies on concomitant mechanisms involved in cell wall assembly/disassembly during fruit development and storage.
27630120	4	45	theme	related	670:676	arg1	members					688:694	related annotated members	670:694	related annotated members of gene families involved in cell wall polysaccharide composition and structural changes during apple fruit growth and ripening	670:822	RESULTS Combined transcriptomic and biochemical analyses allowed the identification of putative enzymes and related annotated members of gene families involved in cell wall polysaccharide composition and structural changes during apple fruit growth and ripening.
27630120	11	46	theme	further	1749:1755	arg1	studies					1757:1763	further studies	1749:1763	further studies on concomitant mechanisms involved in cell wall assembly/disassembly during fruit development and storage	1749:1869	Moreover, new insights into the potential role of peroxidases and transporters, along with cell wall metabolism open the way to further studies on concomitant mechanisms involved in cell wall assembly/disassembly during fruit development and storage.
27630120	5	47	dep	wall	881:884	arg1	degradation					903:913	degradation	903:913	degradation	903:913	The early development genes were mainly related to cell wall biosynthesis and degradation with a particular target on hemicelluloses.
27630120	5	47	dep	wall	881:884	arg1	biosynthesis					886:897	biosynthesis	886:897	biosynthesis	886:897	The early development genes were mainly related to cell wall biosynthesis and degradation with a particular target on hemicelluloses.
27630120	7	48	theme	expansin	1182:1189	arg1	genes					1191:1195	expansin genes	1182:1195	expansin genes	1182:1195	In contrast, fewer genes related to pectin metabolism and cell expansion (expansin genes) were observed in ripening fruit combined with expected changes in cell wall polysaccharide composition.
27630120	7	48	theme	expansin	1182:1189	arg1	genes					1127:1131	fewer genes	1121:1131	fewer genes related to pectin metabolism and cell expansion (expansin genes)	1121:1196	In contrast, fewer genes related to pectin metabolism and cell expansion (expansin genes) were observed in ripening fruit combined with expected changes in cell wall polysaccharide composition.
27630120	2	49	theme	polysaccharide	286:299	arg1	remodelling					301:311	Cell wall polysaccharide remodelling	276:311	Cell wall polysaccharide remodelling	276:311	Cell wall polysaccharide remodelling largely contributes to the elaboration of fleshy fruit texture.
27630120	1	50	theme	biochemical	170:180	arg1	events					182:187	biochemical events	170:187	biochemical events that modify appearance, flavour and texture throughout fruit development and ripening	170:273	BACKGROUND Fruit quality depends on a series of biochemical events that modify appearance, flavour and texture throughout fruit development and ripening.
27630120	6	51	theme	structural	964:973	arg1	evolutions					975:984	Fine structural evolutions	959:984	Fine structural evolutions of galactoglucomannan	959:1006	Fine structural evolutions of galactoglucomannan were strongly correlated with mannan synthase, glucanase (GH9) and β-galactosidase gene expression.
27630120	8	52	theme	structural	1343:1352	arg1	changes					1354:1360	major structural changes	1337:1360	major structural changes	1337:1360	CONCLUSIONS Hemicelluloses undergo major structural changes particularly during early fruit development.
27630120	11	53	theme	wall	1808:1811	arg1	assembly/disassembly					1813:1832	cell wall assembly/disassembly	1803:1832	cell wall assembly/disassembly during fruit development and storage	1803:1869	Moreover, new insights into the potential role of peroxidases and transporters, along with cell wall metabolism open the way to further studies on concomitant mechanisms involved in cell wall assembly/disassembly during fruit development and storage.
27630120	11	54	theme	wall	1717:1720	arg1	metabolism					1722:1731	cell wall metabolism	1712:1731	cell wall metabolism	1712:1731	Moreover, new insights into the potential role of peroxidases and transporters, along with cell wall metabolism open the way to further studies on concomitant mechanisms involved in cell wall assembly/disassembly during fruit development and storage.
27630120	11	55	from	studies	1757:1763	arg1	mechanisms					1780:1789	concomitant mechanisms	1768:1789	concomitant mechanisms involved in cell wall assembly/disassembly during fruit development and storage	1768:1869	Moreover, new insights into the potential role of peroxidases and transporters, along with cell wall metabolism open the way to further studies on concomitant mechanisms involved in cell wall assembly/disassembly during fruit development and storage.
27630120	4	56	theme	cell	725:728	arg1	wall					730:733	cell wall polysaccharide composition and structural changes during apple fruit growth and ripening	725:822	wall	730:733	RESULTS Combined transcriptomic and biochemical analyses allowed the identification of putative enzymes and related annotated members of gene families involved in cell wall polysaccharide composition and structural changes during apple fruit growth and ripening.
27630120	8	57	theme	early	1382:1386	arg1	development					1394:1404	early fruit development	1382:1404	early fruit development	1382:1404	CONCLUSIONS Hemicelluloses undergo major structural changes particularly during early fruit development.
27630120	7	58	theme	pectin	1144:1149	arg1	metabolism					1151:1160	pectin metabolism	1144:1160	pectin metabolism	1144:1160	In contrast, fewer genes related to pectin metabolism and cell expansion (expansin genes) were observed in ripening fruit combined with expected changes in cell wall polysaccharide composition.
27630120	8	59	theme	CONCLUSIONS	1302:1312	arg1	Hemicelluloses					1314:1327	CONCLUSIONS Hemicelluloses	1302:1327	CONCLUSIONS Hemicelluloses	1302:1327	CONCLUSIONS Hemicelluloses undergo major structural changes particularly during early fruit development.
27630120	4	60	theme	gene	699:702	arg1	families					704:711	gene families	699:711	gene families involved in cell wall polysaccharide composition and structural changes during apple fruit growth and ripening	699:822	RESULTS Combined transcriptomic and biochemical analyses allowed the identification of putative enzymes and related annotated members of gene families involved in cell wall polysaccharide composition and structural changes during apple fruit growth and ripening.
27630120	7	61	theme	related	1133:1139	arg1	genes					1191:1195	expansin genes	1182:1195	expansin genes	1182:1195	In contrast, fewer genes related to pectin metabolism and cell expansion (expansin genes) were observed in ripening fruit combined with expected changes in cell wall polysaccharide composition.
27630120	7	61	theme	related	1133:1139	arg1	genes					1127:1131	fewer genes	1121:1131	fewer genes related to pectin metabolism and cell expansion (expansin genes)	1121:1196	In contrast, fewer genes related to pectin metabolism and cell expansion (expansin genes) were observed in ripening fruit combined with expected changes in cell wall polysaccharide composition.
27630120	1	62	theme	BACKGROUND	122:131	arg1	quality					139:145	BACKGROUND Fruit quality	122:145	BACKGROUND Fruit quality	122:145	BACKGROUND Fruit quality depends on a series of biochemical events that modify appearance, flavour and texture throughout fruit development and ripening.
27630120	6	63	theme	mannan	1038:1043	arg1	synthase					1045:1052	mannan synthase	1038:1052	mannan synthase	1038:1052	Fine structural evolutions of galactoglucomannan were strongly correlated with mannan synthase, glucanase (GH9) and β-galactosidase gene expression.
27630120	7	64	theme	fewer	1121:1125	arg1	genes					1191:1195	expansin genes	1182:1195	expansin genes	1182:1195	In contrast, fewer genes related to pectin metabolism and cell expansion (expansin genes) were observed in ripening fruit combined with expected changes in cell wall polysaccharide composition.
27630120	7	64	theme	fewer	1121:1125	arg1	genes					1127:1131	fewer genes	1121:1131	fewer genes related to pectin metabolism and cell expansion (expansin genes)	1121:1196	In contrast, fewer genes related to pectin metabolism and cell expansion (expansin genes) were observed in ripening fruit combined with expected changes in cell wall polysaccharide composition.
27630120	2	65	theme	Cell	276:279	arg1	remodelling					301:311	Cell wall polysaccharide remodelling	276:311	Cell wall polysaccharide remodelling	276:311	Cell wall polysaccharide remodelling largely contributes to the elaboration of fleshy fruit texture.
27630120	4	66	theme	apple	792:796	arg1	growth					804:809	apple fruit growth	792:809	apple fruit growth	792:809	RESULTS Combined transcriptomic and biochemical analyses allowed the identification of putative enzymes and related annotated members of gene families involved in cell wall polysaccharide composition and structural changes during apple fruit growth and ripening.
27630120	0	67	theme	hemicellulose	65:77	arg1	modifications					79:91	hemicellulose modifications	65:91	hemicellulose modifications	65:91	Cell wall dynamics during apple development and storage involves hemicellulose modifications and related expressed genes.
27630120	4	68	theme	polysaccharide	735:748	arg1	composition					750:760	polysaccharide composition	735:760	polysaccharide composition	735:760	RESULTS Combined transcriptomic and biochemical analyses allowed the identification of putative enzymes and related annotated members of gene families involved in cell wall polysaccharide composition and structural changes during apple fruit growth and ripening.
27630120	5	69	theme	development	835:845	arg1	genes					847:851	The early development genes	825:851	The early development genes	825:851	The early development genes were mainly related to cell wall biosynthesis and degradation with a particular target on hemicelluloses.
27630120	9	70	theme	high	1411:1414	arg1	number					1416:1421	The high number	1407:1421	The high number of early expressed β-galactosidase genes	1407:1462	The high number of early expressed β-galactosidase genes questions their function on galactosylated structures during fruit development and storage.
27630120	0	71	theme	expressed	105:113	arg1	genes					115:119	related expressed genes	97:119	related expressed genes	97:119	Cell wall dynamics during apple development and storage involves hemicellulose modifications and related expressed genes.
27630120	6	72	theme	β-galactosidase	1075:1089	arg1	expression					1096:1105	β-galactosidase gene expression	1075:1105	β-galactosidase gene expression	1075:1105	Fine structural evolutions of galactoglucomannan were strongly correlated with mannan synthase, glucanase (GH9) and β-galactosidase gene expression.
27630120	10	73	theme	wall	1580:1583	arg1	substrate					1585:1593	cell wall substrate	1575:1593	cell wall substrate	1575:1593	Their activity and cell wall substrate remains to be identified.
27630120	7	74	theme	wall	1269:1272	arg1	composition					1289:1299	cell wall polysaccharide composition	1264:1299	cell wall polysaccharide composition	1264:1299	In contrast, fewer genes related to pectin metabolism and cell expansion (expansin genes) were observed in ripening fruit combined with expected changes in cell wall polysaccharide composition.
27630120	9	75	theme	expressed	1432:1440	arg1	genes					1458:1462	early expressed β-galactosidase genes	1426:1462	early expressed β-galactosidase genes	1426:1462	The high number of early expressed β-galactosidase genes questions their function on galactosylated structures during fruit development and storage.
27630120	9	76	from	function	1480:1487	arg1	structures					1507:1516	galactosylated structures	1492:1516	galactosylated structures	1492:1516	The high number of early expressed β-galactosidase genes questions their function on galactosylated structures during fruit development and storage.
27630120	4	77	theme	members	688:694	arg1	identification					631:644	the identification	627:644	the identification of putative enzymes and related annotated members of gene families involved in cell wall polysaccharide composition and structural changes during apple fruit growth and ripening	627:822	RESULTS Combined transcriptomic and biochemical analyses allowed the identification of putative enzymes and related annotated members of gene families involved in cell wall polysaccharide composition and structural changes during apple fruit growth and ripening.
27630120	5	78	theme	cell	876:879	arg1	wall					881:884	cell wall biosynthesis and degradation	876:913	cell wall biosynthesis and degradation	876:913	The early development genes were mainly related to cell wall biosynthesis and degradation with a particular target on hemicelluloses.
27630120	9	79	theme	genes	1458:1462	arg1	number					1416:1421	The high number	1407:1421	The high number of early expressed β-galactosidase genes	1407:1462	The high number of early expressed β-galactosidase genes questions their function on galactosylated structures during fruit development and storage.
27630120	4	80	theme	biochemical	598:608	arg1	analyses					610:617	biochemical analyses	598:617	biochemical analyses	598:617	RESULTS Combined transcriptomic and biochemical analyses allowed the identification of putative enzymes and related annotated members of gene families involved in cell wall polysaccharide composition and structural changes during apple fruit growth and ripening.
27630120	7	81	theme	expected	1244:1251	arg1	changes					1253:1259	expected changes	1244:1259	expected changes in cell wall polysaccharide composition	1244:1299	In contrast, fewer genes related to pectin metabolism and cell expansion (expansin genes) were observed in ripening fruit combined with expected changes in cell wall polysaccharide composition.
27630120	11	82	theme	fruit	1841:1845	arg1	development					1847:1857	fruit development	1841:1857	fruit development	1841:1857	Moreover, new insights into the potential role of peroxidases and transporters, along with cell wall metabolism open the way to further studies on concomitant mechanisms involved in cell wall assembly/disassembly during fruit development and storage.
27630120	3	83	theme	wall	429:432	arg1	biosynthesis					449:460	cell wall polysaccharide biosynthesis	424:460	cell wall polysaccharide biosynthesis	424:460	Although several genes and enzymes involved in cell wall polysaccharide biosynthesis and modifications are known, their coordinated activity in these processes is yet to be discovered.
27630120	4	84	theme	transcriptomic	579:592	arg1	RESULTS					562:568	RESULTS	562:568	RESULTS Combined transcriptomic	562:592	RESULTS Combined transcriptomic and biochemical analyses allowed the identification of putative enzymes and related annotated members of gene families involved in cell wall polysaccharide composition and structural changes during apple fruit growth and ripening.
27630120	2	85	theme	fruit	362:366	arg1	texture					368:374	fleshy fruit texture	355:374	fleshy fruit texture	355:374	Cell wall polysaccharide remodelling largely contributes to the elaboration of fleshy fruit texture.
27630120	9	86	theme	fruit	1525:1529	arg1	development					1531:1541	fruit development	1525:1541	fruit development	1525:1541	The high number of early expressed β-galactosidase genes questions their function on galactosylated structures during fruit development and storage.
27630120	4	87	dep	wall	730:733	arg1	composition					750:760	polysaccharide composition	735:760	polysaccharide composition	735:760	RESULTS Combined transcriptomic and biochemical analyses allowed the identification of putative enzymes and related annotated members of gene families involved in cell wall polysaccharide composition and structural changes during apple fruit growth and ripening.
27630120	4	88	theme	annotated	678:686	arg1	members					688:694	related annotated members	670:694	related annotated members of gene families involved in cell wall polysaccharide composition and structural changes during apple fruit growth and ripening	670:822	RESULTS Combined transcriptomic and biochemical analyses allowed the identification of putative enzymes and related annotated members of gene families involved in cell wall polysaccharide composition and structural changes during apple fruit growth and ripening.
27630120	0	89	theme	wall	5:8	arg1	dynamics					10:17	Cell wall dynamics	0:17	Cell wall dynamics during apple development and storage	0:54	Cell wall dynamics during apple development and storage involves hemicellulose modifications and related expressed genes.
24859193	7	0	theme	restricted	1130:1139	arg1	mobility					1151:1158	the restricted molecular mobility	1126:1158	the restricted molecular mobility of water, which acted as a catalytic species for the degradation,	1126:1224	These results suggested that the stabilization of Limaprost by β-CD was at least partly due to the restricted molecular mobility of water, which acted as a catalytic species for the degradation, and also to the protection of the five-membered ring of Limaprost from water catalytic dehydration through inclusion complex formation with β-CD.
24859193	2	1	theme	Limaprost	319:327	arg1	alfadex/dextran					329:343	Limaprost alfadex/dextran 40	319:346	Limaprost alfadex/dextran 40	319:346	Aqueous solutions of Limaprost alfadex/dextran 40 were lyophilized with and without β-CD.
24859193	6	2	theme	spectroscopic	928:940	arg1	studies					942:948	Solid (2)H-NMR spectroscopic studies	913:948	Solid (2)H-NMR spectroscopic studies	913:948	Solid (2)H-NMR spectroscopic studies showed that β-CD constrained the molecular mobility of water in the solid state.
24859193	7	3	theme	molecular	1141:1149	arg1	mobility					1151:1158	the restricted molecular mobility	1126:1158	the restricted molecular mobility of water, which acted as a catalytic species for the degradation,	1126:1224	These results suggested that the stabilization of Limaprost by β-CD was at least partly due to the restricted molecular mobility of water, which acted as a catalytic species for the degradation, and also to the protection of the five-membered ring of Limaprost from water catalytic dehydration through inclusion complex formation with β-CD.
24859193	0	4	from	effect	12:17	arg1	Limaprost					40:48	Limaprost	40:48	Limaprost	40:48	Stabilizing effect of β-cyclodextrin on Limaprost, a PGE₁ derivative, in Limaprost alfadex tablets (Opalmon) in highly humid conditions.
24859193	0	4	from	effect	12:17	arg1	derivative					58:67	a PGE₁ derivative	51:67	a PGE₁ derivative	51:67	Stabilizing effect of β-cyclodextrin on Limaprost, a PGE₁ derivative, in Limaprost alfadex tablets (Opalmon) in highly humid conditions.
24859193	4	5	theme	moisture-resistant	659:676	arg1	formulation					678:688	this moisture-resistant formulation	654:688	this moisture-resistant formulation	654:688	Moreover, the addition of β-CD as an excipient to tablets of these lyophilized composites remarkably improved the stability of Limaprost, and Limaprost in this moisture-resistant formulation was chemically stable for 19 weeks at 30°C, 75% relative humidity (R.H.).
24859193	6	6	theme	H-NMR	922:926	arg1	studies					942:948	Solid (2)H-NMR spectroscopic studies	913:948	Solid (2)H-NMR spectroscopic studies	913:948	Solid (2)H-NMR spectroscopic studies showed that β-CD constrained the molecular mobility of water in the solid state.
24859193	0	7	theme	alfadex	83:89	arg1	Opalmon					100:106	Opalmon	100:106	Opalmon	100:106	Stabilizing effect of β-cyclodextrin on Limaprost, a PGE₁ derivative, in Limaprost alfadex tablets (Opalmon) in highly humid conditions.
24859193	0	7	theme	alfadex	83:89	arg1	tablets					91:97	Limaprost alfadex tablets	73:97	Limaprost alfadex tablets (Opalmon)	73:107	Stabilizing effect of β-cyclodextrin on Limaprost, a PGE₁ derivative, in Limaprost alfadex tablets (Opalmon) in highly humid conditions.
24859193	4	8	theme	75	734:735	arg1	%					736:736	%	736:736	%	736:736	Moreover, the addition of β-CD as an excipient to tablets of these lyophilized composites remarkably improved the stability of Limaprost, and Limaprost in this moisture-resistant formulation was chemically stable for 19 weeks at 30°C, 75% relative humidity (R.H.).
24859193	7	9	theme	catalytic	1303:1311	arg1	dehydration					1313:1323	water catalytic dehydration	1297:1323	water catalytic dehydration through inclusion complex formation with β-CD	1297:1369	These results suggested that the stabilization of Limaprost by β-CD was at least partly due to the restricted molecular mobility of water, which acted as a catalytic species for the degradation, and also to the protection of the five-membered ring of Limaprost from water catalytic dehydration through inclusion complex formation with β-CD.
24859193	0	10	theme	Limaprost	73:81	arg1	Opalmon					100:106	Opalmon	100:106	Opalmon	100:106	Stabilizing effect of β-cyclodextrin on Limaprost, a PGE₁ derivative, in Limaprost alfadex tablets (Opalmon) in highly humid conditions.
24859193	0	10	theme	Limaprost	73:81	arg1	tablets					91:97	Limaprost alfadex tablets	73:97	Limaprost alfadex tablets (Opalmon)	73:107	Stabilizing effect of β-cyclodextrin on Limaprost, a PGE₁ derivative, in Limaprost alfadex tablets (Opalmon) in highly humid conditions.
24859193	7	11	theme	water	1163:1167	arg1	mobility					1151:1158	the restricted molecular mobility	1126:1158	the restricted molecular mobility of water, which acted as a catalytic species for the degradation,	1126:1224	These results suggested that the stabilization of Limaprost by β-CD was at least partly due to the restricted molecular mobility of water, which acted as a catalytic species for the degradation, and also to the protection of the five-membered ring of Limaprost from water catalytic dehydration through inclusion complex formation with β-CD.
24859193	4	12	theme	relative	738:745	arg1	R.H.					757:760	R.H.	757:760	R.H.	757:760	Moreover, the addition of β-CD as an excipient to tablets of these lyophilized composites remarkably improved the stability of Limaprost, and Limaprost in this moisture-resistant formulation was chemically stable for 19 weeks at 30°C, 75% relative humidity (R.H.).
24859193	4	12	theme	relative	738:745	arg1	humidity					747:754	75% relative humidity	734:754	75% relative humidity (R.H.)	734:761	Moreover, the addition of β-CD as an excipient to tablets of these lyophilized composites remarkably improved the stability of Limaprost, and Limaprost in this moisture-resistant formulation was chemically stable for 19 weeks at 30°C, 75% relative humidity (R.H.).
24859193	4	12	theme	relative	738:745	arg1	30°C					728:731	30°C	728:731	30°C	728:731	Moreover, the addition of β-CD as an excipient to tablets of these lyophilized composites remarkably improved the stability of Limaprost, and Limaprost in this moisture-resistant formulation was chemically stable for 19 weeks at 30°C, 75% relative humidity (R.H.).
24859193	6	13	theme	water	1005:1009	arg1	mobility					993:1000	the molecular mobility	979:1000	the molecular mobility of water in the solid state	979:1028	Solid (2)H-NMR spectroscopic studies showed that β-CD constrained the molecular mobility of water in the solid state.
24859193	5	14	theme	products	815:822	arg1	analysis					773:780	Chemical analysis	764:780	Chemical analysis of Limaprost and its degradation products	764:822	Chemical analysis of Limaprost and its degradation products indicated that degradation proceeded in the inclusion form (i.e., within the CD cavity).
24859193	0	15	from	Limaprost	40:48	arg1	Opalmon					100:106	Opalmon	100:106	Opalmon	100:106	Stabilizing effect of β-cyclodextrin on Limaprost, a PGE₁ derivative, in Limaprost alfadex tablets (Opalmon) in highly humid conditions.
24859193	0	15	from	Limaprost	40:48	arg1	tablets					91:97	Limaprost alfadex tablets	73:97	Limaprost alfadex tablets (Opalmon)	73:107	Stabilizing effect of β-cyclodextrin on Limaprost, a PGE₁ derivative, in Limaprost alfadex tablets (Opalmon) in highly humid conditions.
24859193	4	16	from	30°C	728:731	arg1	stable					705:710	stable	705:710	stable	705:710	Moreover, the addition of β-CD as an excipient to tablets of these lyophilized composites remarkably improved the stability of Limaprost, and Limaprost in this moisture-resistant formulation was chemically stable for 19 weeks at 30°C, 75% relative humidity (R.H.).
24859193	4	17	theme	%	736:736	arg1	R.H.					757:760	R.H.	757:760	R.H.	757:760	Moreover, the addition of β-CD as an excipient to tablets of these lyophilized composites remarkably improved the stability of Limaprost, and Limaprost in this moisture-resistant formulation was chemically stable for 19 weeks at 30°C, 75% relative humidity (R.H.).
24859193	4	17	theme	%	736:736	arg1	humidity					747:754	75% relative humidity	734:754	75% relative humidity (R.H.)	734:761	Moreover, the addition of β-CD as an excipient to tablets of these lyophilized composites remarkably improved the stability of Limaprost, and Limaprost in this moisture-resistant formulation was chemically stable for 19 weeks at 30°C, 75% relative humidity (R.H.).
24859193	4	17	theme	%	736:736	arg1	30°C					728:731	30°C	728:731	30°C	728:731	Moreover, the addition of β-CD as an excipient to tablets of these lyophilized composites remarkably improved the stability of Limaprost, and Limaprost in this moisture-resistant formulation was chemically stable for 19 weeks at 30°C, 75% relative humidity (R.H.).
24859193	6	18	theme	solid	1018:1022	arg1	state					1024:1028	the solid state	1014:1028	the solid state	1014:1028	Solid (2)H-NMR spectroscopic studies showed that β-CD constrained the molecular mobility of water in the solid state.
24859193	4	19	theme	Limaprost	626:634	arg1	stability					613:621	the stability	609:621	the stability of Limaprost, and Limaprost in this moisture-resistant formulation was chemically stable for 19 weeks at 30°C, 75% relative humidity (R.H.)	609:761	Moreover, the addition of β-CD as an excipient to tablets of these lyophilized composites remarkably improved the stability of Limaprost, and Limaprost in this moisture-resistant formulation was chemically stable for 19 weeks at 30°C, 75% relative humidity (R.H.).
24859193	4	20	theme	Limaprost	641:649	arg1	stability					613:621	the stability	609:621	the stability of Limaprost, and Limaprost in this moisture-resistant formulation was chemically stable for 19 weeks at 30°C, 75% relative humidity (R.H.)	609:761	Moreover, the addition of β-CD as an excipient to tablets of these lyophilized composites remarkably improved the stability of Limaprost, and Limaprost in this moisture-resistant formulation was chemically stable for 19 weeks at 30°C, 75% relative humidity (R.H.).
24859193	5	21	theme	Chemical	764:771	arg1	analysis					773:780	Chemical analysis	764:780	Chemical analysis of Limaprost and its degradation products	764:822	Chemical analysis of Limaprost and its degradation products indicated that degradation proceeded in the inclusion form (i.e., within the CD cavity).
24859193	4	22	theme	β-CD	525:528	arg1	addition					513:520	the addition	509:520	the addition of β-CD as an excipient to tablets of these lyophilized composites	509:587	Moreover, the addition of β-CD as an excipient to tablets of these lyophilized composites remarkably improved the stability of Limaprost, and Limaprost in this moisture-resistant formulation was chemically stable for 19 weeks at 30°C, 75% relative humidity (R.H.).
24859193	3	23	from	conditions	464:473	arg1	stable					448:453	stable	448:453	stable	448:453	Limaprost alfadex lyophilized with β-CD was more chemically stable in humid conditions than that without β-CD.
24859193	1	24	theme	Limaprost	171:179	arg1	Stabilization					137:149	Stabilization	137:149	Stabilization against humidity of Limaprost (a prostaglandin E₁ derivative), which is currently marketed as Opalmon,	137:252	Stabilization against humidity of Limaprost (a prostaglandin E₁ derivative), which is currently marketed as Opalmon, was undertaken using β-cyclodextrin (β-CD).
24859193	0	25	theme	β-cyclodextrin	22:35	arg1	effect					12:17	effect	12:17	effect of β-cyclodextrin on Limaprost, a PGE₁ derivative, in Limaprost alfadex tablets (Opalmon)	12:107	Stabilizing effect of β-cyclodextrin on Limaprost, a PGE₁ derivative, in Limaprost alfadex tablets (Opalmon) in highly humid conditions.
24859193	6	26	theme	Solid	913:917	arg1	studies					942:948	Solid (2)H-NMR spectroscopic studies	913:948	Solid (2)H-NMR spectroscopic studies	913:948	Solid (2)H-NMR spectroscopic studies showed that β-CD constrained the molecular mobility of water in the solid state.
24859193	5	27	theme	CD	901:902	arg1	cavity					904:909	the CD cavity	897:909	the CD cavity	897:909	Chemical analysis of Limaprost and its degradation products indicated that degradation proceeded in the inclusion form (i.e., within the CD cavity).
24859193	5	28	theme	Limaprost	785:793	arg1	analysis					773:780	Chemical analysis	764:780	Chemical analysis of Limaprost and its degradation products	764:822	Chemical analysis of Limaprost and its degradation products indicated that degradation proceeded in the inclusion form (i.e., within the CD cavity).
24859193	7	29	theme	inclusion	1333:1341	arg1	formation					1351:1359	inclusion complex formation	1333:1359	inclusion complex formation with β-CD	1333:1369	These results suggested that the stabilization of Limaprost by β-CD was at least partly due to the restricted molecular mobility of water, which acted as a catalytic species for the degradation, and also to the protection of the five-membered ring of Limaprost from water catalytic dehydration through inclusion complex formation with β-CD.
24859193	5	30	theme	inclusion	868:876	arg1	form					878:881	the inclusion form	864:881	the inclusion form	864:881	Chemical analysis of Limaprost and its degradation products indicated that degradation proceeded in the inclusion form (i.e., within the CD cavity).
24859193	6	31	theme	molecular	983:991	arg1	mobility					993:1000	the molecular mobility	979:1000	the molecular mobility of water in the solid state	979:1028	Solid (2)H-NMR spectroscopic studies showed that β-CD constrained the molecular mobility of water in the solid state.
24859193	7	32	theme	Limaprost	1081:1089	arg1	stabilization					1064:1076	the stabilization	1060:1076	the stabilization of Limaprost by β-CD	1060:1097	These results suggested that the stabilization of Limaprost by β-CD was at least partly due to the restricted molecular mobility of water, which acted as a catalytic species for the degradation, and also to the protection of the five-membered ring of Limaprost from water catalytic dehydration through inclusion complex formation with β-CD.
24859193	7	32	theme	Limaprost	1081:1089	arg1	due					1119:1121	due	1119:1121	due	1119:1121	These results suggested that the stabilization of Limaprost by β-CD was at least partly due to the restricted molecular mobility of water, which acted as a catalytic species for the degradation, and also to the protection of the five-membered ring of Limaprost from water catalytic dehydration through inclusion complex formation with β-CD.
24859193	6	33	from	mobility	993:1000	arg1	state					1024:1028	the solid state	1014:1028	the solid state	1014:1028	Solid (2)H-NMR spectroscopic studies showed that β-CD constrained the molecular mobility of water in the solid state.
24859193	7	34	theme	Limaprost	1282:1290	arg1	ring					1274:1277	the five-membered ring	1256:1277	the five-membered ring of Limaprost	1256:1290	These results suggested that the stabilization of Limaprost by β-CD was at least partly due to the restricted molecular mobility of water, which acted as a catalytic species for the degradation, and also to the protection of the five-membered ring of Limaprost from water catalytic dehydration through inclusion complex formation with β-CD.
24859193	1	35	theme	prostaglandin	184:196	arg1	derivative					201:210	a prostaglandin E₁ derivative	182:210	a prostaglandin E₁ derivative	182:210	Stabilization against humidity of Limaprost (a prostaglandin E₁ derivative), which is currently marketed as Opalmon, was undertaken using β-cyclodextrin (β-CD).
24859193	1	35	theme	prostaglandin	184:196	arg1	Limaprost					171:179	Limaprost	171:179	Limaprost (a prostaglandin E₁ derivative)	171:211	Stabilization against humidity of Limaprost (a prostaglandin E₁ derivative), which is currently marketed as Opalmon, was undertaken using β-cyclodextrin (β-CD).
24859193	3	36	theme	Limaprost	388:396	arg1	alfadex					398:404	Limaprost alfadex	388:404	Limaprost alfadex lyophilized with β-CD	388:426	Limaprost alfadex lyophilized with β-CD was more chemically stable in humid conditions than that without β-CD.
24859193	7	37	theme	water	1297:1301	arg1	dehydration					1313:1323	water catalytic dehydration	1297:1323	water catalytic dehydration through inclusion complex formation with β-CD	1297:1369	These results suggested that the stabilization of Limaprost by β-CD was at least partly due to the restricted molecular mobility of water, which acted as a catalytic species for the degradation, and also to the protection of the five-membered ring of Limaprost from water catalytic dehydration through inclusion complex formation with β-CD.
24859193	1	38	theme	E₁	198:199	arg1	derivative					201:210	a prostaglandin E₁ derivative	182:210	a prostaglandin E₁ derivative	182:210	Stabilization against humidity of Limaprost (a prostaglandin E₁ derivative), which is currently marketed as Opalmon, was undertaken using β-cyclodextrin (β-CD).
24859193	1	38	theme	E₁	198:199	arg1	Limaprost					171:179	Limaprost	171:179	Limaprost (a prostaglandin E₁ derivative)	171:211	Stabilization against humidity of Limaprost (a prostaglandin E₁ derivative), which is currently marketed as Opalmon, was undertaken using β-cyclodextrin (β-CD).
24859193	7	39	with	formation	1351:1359	arg1	β-CD					1366:1369	β-CD	1366:1369	β-CD	1366:1369	These results suggested that the stabilization of Limaprost by β-CD was at least partly due to the restricted molecular mobility of water, which acted as a catalytic species for the degradation, and also to the protection of the five-membered ring of Limaprost from water catalytic dehydration through inclusion complex formation with β-CD.
24859193	7	40	theme	ring	1274:1277	arg1	protection					1242:1251	the protection	1238:1251	the protection of the five-membered ring of Limaprost from water catalytic dehydration through inclusion complex formation with β-CD	1238:1369	These results suggested that the stabilization of Limaprost by β-CD was at least partly due to the restricted molecular mobility of water, which acted as a catalytic species for the degradation, and also to the protection of the five-membered ring of Limaprost from water catalytic dehydration through inclusion complex formation with β-CD.
24859193	7	41	theme	catalytic	1187:1195	arg1	water					1163:1167	water	1163:1167	water	1163:1167	These results suggested that the stabilization of Limaprost by β-CD was at least partly due to the restricted molecular mobility of water, which acted as a catalytic species for the degradation, and also to the protection of the five-membered ring of Limaprost from water catalytic dehydration through inclusion complex formation with β-CD.
24859193	7	41	theme	catalytic	1187:1195	arg1	species					1197:1203	a catalytic species	1185:1203	a catalytic species for the degradation	1185:1223	These results suggested that the stabilization of Limaprost by β-CD was at least partly due to the restricted molecular mobility of water, which acted as a catalytic species for the degradation, and also to the protection of the five-membered ring of Limaprost from water catalytic dehydration through inclusion complex formation with β-CD.
24859193	0	42	theme	humid	119:123	arg1	conditions					125:134	highly humid conditions	112:134	highly humid conditions	112:134	Stabilizing effect of β-cyclodextrin on Limaprost, a PGE₁ derivative, in Limaprost alfadex tablets (Opalmon) in highly humid conditions.
24859193	2	43	theme	Aqueous	298:304	arg1	solutions					306:314	Aqueous solutions	298:314	Aqueous solutions of Limaprost alfadex/dextran 40	298:346	Aqueous solutions of Limaprost alfadex/dextran 40 were lyophilized with and without β-CD.
24859193	3	44	from	stable	448:453	arg1	conditions					464:473	humid conditions	458:473	humid conditions	458:473	Limaprost alfadex lyophilized with β-CD was more chemically stable in humid conditions than that without β-CD.
24859193	5	45	dep	proceeded	851:859	arg1	i.e.					884:887	i.e.	884:887	i.e.	884:887	Chemical analysis of Limaprost and its degradation products indicated that degradation proceeded in the inclusion form (i.e., within the CD cavity).
24859193	4	46	theme	lyophilized	566:576	arg1	composites					578:587	these lyophilized composites	560:587	these lyophilized composites	560:587	Moreover, the addition of β-CD as an excipient to tablets of these lyophilized composites remarkably improved the stability of Limaprost, and Limaprost in this moisture-resistant formulation was chemically stable for 19 weeks at 30°C, 75% relative humidity (R.H.).
24859193	7	47	theme	complex	1343:1349	arg1	formation					1351:1359	inclusion complex formation	1333:1359	inclusion complex formation with β-CD	1333:1369	These results suggested that the stabilization of Limaprost by β-CD was at least partly due to the restricted molecular mobility of water, which acted as a catalytic species for the degradation, and also to the protection of the five-membered ring of Limaprost from water catalytic dehydration through inclusion complex formation with β-CD.
24859193	7	48	theme	five-membered	1260:1272	arg1	ring					1274:1277	the five-membered ring	1256:1277	the five-membered ring of Limaprost	1256:1290	These results suggested that the stabilization of Limaprost by β-CD was at least partly due to the restricted molecular mobility of water, which acted as a catalytic species for the degradation, and also to the protection of the five-membered ring of Limaprost from water catalytic dehydration through inclusion complex formation with β-CD.
24859193	3	49	theme	humid	458:462	arg1	conditions					464:473	humid conditions	458:473	humid conditions	458:473	Limaprost alfadex lyophilized with β-CD was more chemically stable in humid conditions than that without β-CD.
24859193	7	50	from	dehydration	1313:1323	arg1	protection					1242:1251	the protection	1238:1251	the protection of the five-membered ring of Limaprost from water catalytic dehydration through inclusion complex formation with β-CD	1238:1369	These results suggested that the stabilization of Limaprost by β-CD was at least partly due to the restricted molecular mobility of water, which acted as a catalytic species for the degradation, and also to the protection of the five-membered ring of Limaprost from water catalytic dehydration through inclusion complex formation with β-CD.
24859193	5	51	theme	degradation	803:813	arg1	products					815:822	its degradation products	799:822	its degradation products	799:822	Chemical analysis of Limaprost and its degradation products indicated that degradation proceeded in the inclusion form (i.e., within the CD cavity).
24859193	0	52	theme	PGE₁	53:56	arg1	Limaprost					40:48	Limaprost	40:48	Limaprost	40:48	Stabilizing effect of β-cyclodextrin on Limaprost, a PGE₁ derivative, in Limaprost alfadex tablets (Opalmon) in highly humid conditions.
24859193	0	52	theme	PGE₁	53:56	arg1	derivative					58:67	a PGE₁ derivative	51:67	a PGE₁ derivative	51:67	Stabilizing effect of β-cyclodextrin on Limaprost, a PGE₁ derivative, in Limaprost alfadex tablets (Opalmon) in highly humid conditions.
24859193	4	53	dep	Limaprost	626:634	arg1	stable					705:710	stable	705:710	stable	705:710	Moreover, the addition of β-CD as an excipient to tablets of these lyophilized composites remarkably improved the stability of Limaprost, and Limaprost in this moisture-resistant formulation was chemically stable for 19 weeks at 30°C, 75% relative humidity (R.H.).
24859193	2	54	theme	alfadex/dextran	329:343	arg1	solutions					306:314	Aqueous solutions	298:314	Aqueous solutions of Limaprost alfadex/dextran 40	298:346	Aqueous solutions of Limaprost alfadex/dextran 40 were lyophilized with and without β-CD.
24859193	4	55	theme	composites	578:587	arg1	tablets					549:555	tablets	549:555	tablets of these lyophilized composites	549:587	Moreover, the addition of β-CD as an excipient to tablets of these lyophilized composites remarkably improved the stability of Limaprost, and Limaprost in this moisture-resistant formulation was chemically stable for 19 weeks at 30°C, 75% relative humidity (R.H.).
27374542	2	0	theme	flours	347:352	arg1	composition					362:372	flours surface composition	347:372	flours surface composition	347:372	The uncooked kernels and flours surface composition of the two selected rice varieties, Thadokkham-11 (TDK11) and Doongara (DG) demonstrated an over-expression of lipids and proteins and an under-expression of starch compared to the bulk composition.
27374542	4	1	theme	gelatinized	857:867	arg1	proteins					887:894	gelatinized starch, denatured proteins	857:894	gelatinized starch, denatured proteins	857:894	Nevertheless, it was unable to distinguish components in cooked rice samples possibly due to complex interactions between gelatinized starch, denatured proteins and lipids.
27374542	2	2	theme	lipids	485:490	arg1	over-expression					466:480	an over-expression	463:480	an over-expression of lipids and proteins	463:503	The uncooked kernels and flours surface composition of the two selected rice varieties, Thadokkham-11 (TDK11) and Doongara (DG) demonstrated an over-expression of lipids and proteins and an under-expression of starch compared to the bulk composition.
27374542	2	2	theme	lipids	485:490	arg1	under-expression					512:527	an under-expression	509:527	an under-expression of starch	509:537	The uncooked kernels and flours surface composition of the two selected rice varieties, Thadokkham-11 (TDK11) and Doongara (DG) demonstrated an over-expression of lipids and proteins and an under-expression of starch compared to the bulk composition.
27374542	0	3	theme	chemical	94:101	arg1	composition					103:113	surface chemical composition	86:113	surface chemical composition	86:113	X-ray photoelectron spectroscopic analysis of rice kernels and flours: Measurement of surface chemical composition.
27374542	0	4	theme	surface	86:92	arg1	composition					103:113	surface chemical composition	86:113	surface chemical composition	86:113	X-ray photoelectron spectroscopic analysis of rice kernels and flours: Measurement of surface chemical composition.
27374542	4	5	theme	complex	828:834	arg1	interactions					836:847	complex interactions	828:847	complex interactions between gelatinized starch, denatured proteins and lipids	828:905	Nevertheless, it was unable to distinguish components in cooked rice samples possibly due to complex interactions between gelatinized starch, denatured proteins and lipids.
27374542	3	6	dep	polysaccharides	645:659	arg1	mainly					662:667	mainly	662:667	mainly	662:667	The results of the study showed that XPS was able to differentiate rice polysaccharides (mainly starch), proteins and lipids in uncooked rice kernels and flours.
27374542	4	7	theme	rice	799:802	arg1	samples					804:810	cooked rice samples	792:810	cooked rice samples	792:810	Nevertheless, it was unable to distinguish components in cooked rice samples possibly due to complex interactions between gelatinized starch, denatured proteins and lipids.
27374542	5	8	theme	Scanning	941:948	arg1	Microscopy					959:968	Scanning Electron Microscopy	941:968	Scanning Electron Microscopy	941:968	High resolution imaging methods (Scanning Electron Microscopy and Confocal Laser Scanning Microscopy) were employed to obtain complementary information about the properties and location of starch, proteins and lipids in rice kernels and flours.
27374542	5	8	theme	Scanning	941:948	arg1	methods					932:938	High resolution imaging methods	908:938	High resolution imaging methods (Scanning Electron Microscopy and Confocal Laser Scanning Microscopy)	908:1008	High resolution imaging methods (Scanning Electron Microscopy and Confocal Laser Scanning Microscopy) were employed to obtain complementary information about the properties and location of starch, proteins and lipids in rice kernels and flours.
27374542	2	9	theme	selected	385:392	arg1	varieties					399:407	the two selected rice varieties	377:407	the two selected rice varieties	377:407	The uncooked kernels and flours surface composition of the two selected rice varieties, Thadokkham-11 (TDK11) and Doongara (DG) demonstrated an over-expression of lipids and proteins and an under-expression of starch compared to the bulk composition.
27374542	2	9	theme	selected	385:392	arg1	Doongara					436:443	Doongara	436:443	Doongara (DG)	436:448	The uncooked kernels and flours surface composition of the two selected rice varieties, Thadokkham-11 (TDK11) and Doongara (DG) demonstrated an over-expression of lipids and proteins and an under-expression of starch compared to the bulk composition.
27374542	2	9	theme	selected	385:392	arg1	Thadokkham-11					410:422	Thadokkham-11	410:422	Thadokkham-11 (TDK11)	410:430	The uncooked kernels and flours surface composition of the two selected rice varieties, Thadokkham-11 (TDK11) and Doongara (DG) demonstrated an over-expression of lipids and proteins and an under-expression of starch compared to the bulk composition.
27374542	0	10	theme	composition	103:113	arg1	Measurement					71:81	Measurement	71:81	X-ray photoelectron spectroscopic analysis of rice kernels and flours: Measurement of surface chemical composition.	0:114	X-ray photoelectron spectroscopic analysis of rice kernels and flours: Measurement of surface chemical composition.
27374542	4	11	theme	cooked	792:797	arg1	samples					804:810	cooked rice samples	792:810	cooked rice samples	792:810	Nevertheless, it was unable to distinguish components in cooked rice samples possibly due to complex interactions between gelatinized starch, denatured proteins and lipids.
27374542	3	12	from	proteins	678:685	arg1	flours					727:732	flours	727:732	flours	727:732	The results of the study showed that XPS was able to differentiate rice polysaccharides (mainly starch), proteins and lipids in uncooked rice kernels and flours.
27374542	3	12	from	proteins	678:685	arg1	kernels					715:721	uncooked rice kernels	701:721	uncooked rice kernels	701:721	The results of the study showed that XPS was able to differentiate rice polysaccharides (mainly starch), proteins and lipids in uncooked rice kernels and flours.
27374542	0	13	theme	photoelectron	6:18	arg1	analysis					34:41	X-ray photoelectron spectroscopic analysis	0:41	X-ray photoelectron spectroscopic analysis of rice kernels and flours: Measurement of surface chemical composition.	0:114	X-ray photoelectron spectroscopic analysis of rice kernels and flours: Measurement of surface chemical composition.
27374542	5	14	theme	starch	1097:1102	arg1	location					1085:1092	location	1085:1092	location	1085:1092	High resolution imaging methods (Scanning Electron Microscopy and Confocal Laser Scanning Microscopy) were employed to obtain complementary information about the properties and location of starch, proteins and lipids in rice kernels and flours.
27374542	5	14	theme	starch	1097:1102	arg1	properties					1070:1079	properties	1070:1079	properties	1070:1079	High resolution imaging methods (Scanning Electron Microscopy and Confocal Laser Scanning Microscopy) were employed to obtain complementary information about the properties and location of starch, proteins and lipids in rice kernels and flours.
27374542	0	15	theme	X-ray	0:4	arg1	analysis					34:41	X-ray photoelectron spectroscopic analysis	0:41	X-ray photoelectron spectroscopic analysis of rice kernels and flours: Measurement of surface chemical composition.	0:114	X-ray photoelectron spectroscopic analysis of rice kernels and flours: Measurement of surface chemical composition.
27374542	3	16	theme	rice	710:713	arg1	kernels					715:721	uncooked rice kernels	701:721	uncooked rice kernels	701:721	The results of the study showed that XPS was able to differentiate rice polysaccharides (mainly starch), proteins and lipids in uncooked rice kernels and flours.
27374542	5	17	theme	High	908:911	arg1	Microscopy					998:1007	Confocal Laser Scanning Microscopy	974:1007	Confocal Laser Scanning Microscopy	974:1007	High resolution imaging methods (Scanning Electron Microscopy and Confocal Laser Scanning Microscopy) were employed to obtain complementary information about the properties and location of starch, proteins and lipids in rice kernels and flours.
27374542	5	17	theme	High	908:911	arg1	Microscopy					959:968	Scanning Electron Microscopy	941:968	Scanning Electron Microscopy	941:968	High resolution imaging methods (Scanning Electron Microscopy and Confocal Laser Scanning Microscopy) were employed to obtain complementary information about the properties and location of starch, proteins and lipids in rice kernels and flours.
27374542	5	17	theme	High	908:911	arg1	methods					932:938	High resolution imaging methods	908:938	High resolution imaging methods (Scanning Electron Microscopy and Confocal Laser Scanning Microscopy)	908:1008	High resolution imaging methods (Scanning Electron Microscopy and Confocal Laser Scanning Microscopy) were employed to obtain complementary information about the properties and location of starch, proteins and lipids in rice kernels and flours.
27374542	5	18	from	proteins	1105:1112	arg1	kernels					1133:1139	rice kernels	1128:1139	rice kernels	1128:1139	High resolution imaging methods (Scanning Electron Microscopy and Confocal Laser Scanning Microscopy) were employed to obtain complementary information about the properties and location of starch, proteins and lipids in rice kernels and flours.
27374542	5	18	from	proteins	1105:1112	arg1	flours					1145:1150	flours	1145:1150	flours	1145:1150	High resolution imaging methods (Scanning Electron Microscopy and Confocal Laser Scanning Microscopy) were employed to obtain complementary information about the properties and location of starch, proteins and lipids in rice kernels and flours.
27374542	1	19	theme	X-ray	177:181	arg1	spectroscopy					197:208	X-ray photoelectron spectroscopy	177:208	X-ray photoelectron spectroscopy (XPS)	177:214	The objectives of this study were to evaluate the ability of X-ray photoelectron spectroscopy (XPS) to differentiate rice macromolecules and to calculate the surface composition of rice kernels and flours.
27374542	1	19	theme	X-ray	177:181	arg1	XPS					211:213	XPS	211:213	XPS	211:213	The objectives of this study were to evaluate the ability of X-ray photoelectron spectroscopy (XPS) to differentiate rice macromolecules and to calculate the surface composition of rice kernels and flours.
27374542	1	20	theme	surface	274:280	arg1	composition					282:292	the surface composition	270:292	the surface composition of rice kernels and flours	270:319	The objectives of this study were to evaluate the ability of X-ray photoelectron spectroscopy (XPS) to differentiate rice macromolecules and to calculate the surface composition of rice kernels and flours.
27374542	2	21	theme	starch	532:537	arg1	over-expression					466:480	an over-expression	463:480	an over-expression of lipids and proteins	463:503	The uncooked kernels and flours surface composition of the two selected rice varieties, Thadokkham-11 (TDK11) and Doongara (DG) demonstrated an over-expression of lipids and proteins and an under-expression of starch compared to the bulk composition.
27374542	2	21	theme	starch	532:537	arg1	under-expression					512:527	an under-expression	509:527	an under-expression of starch	509:537	The uncooked kernels and flours surface composition of the two selected rice varieties, Thadokkham-11 (TDK11) and Doongara (DG) demonstrated an over-expression of lipids and proteins and an under-expression of starch compared to the bulk composition.
27374542	2	22	dep	kernels	335:341	arg1	The					322:324	The	322:324	The	322:324	The uncooked kernels and flours surface composition of the two selected rice varieties, Thadokkham-11 (TDK11) and Doongara (DG) demonstrated an over-expression of lipids and proteins and an under-expression of starch compared to the bulk composition.
27374542	5	23	theme	Scanning	989:996	arg1	Microscopy					998:1007	Confocal Laser Scanning Microscopy	974:1007	Confocal Laser Scanning Microscopy	974:1007	High resolution imaging methods (Scanning Electron Microscopy and Confocal Laser Scanning Microscopy) were employed to obtain complementary information about the properties and location of starch, proteins and lipids in rice kernels and flours.
27374542	5	23	theme	Scanning	989:996	arg1	methods					932:938	High resolution imaging methods	908:938	High resolution imaging methods (Scanning Electron Microscopy and Confocal Laser Scanning Microscopy)	908:1008	High resolution imaging methods (Scanning Electron Microscopy and Confocal Laser Scanning Microscopy) were employed to obtain complementary information about the properties and location of starch, proteins and lipids in rice kernels and flours.
27374542	1	24	theme	photoelectron	183:195	arg1	spectroscopy					197:208	X-ray photoelectron spectroscopy	177:208	X-ray photoelectron spectroscopy (XPS)	177:214	The objectives of this study were to evaluate the ability of X-ray photoelectron spectroscopy (XPS) to differentiate rice macromolecules and to calculate the surface composition of rice kernels and flours.
27374542	1	24	theme	photoelectron	183:195	arg1	XPS					211:213	XPS	211:213	XPS	211:213	The objectives of this study were to evaluate the ability of X-ray photoelectron spectroscopy (XPS) to differentiate rice macromolecules and to calculate the surface composition of rice kernels and flours.
27374542	0	25	theme	spectroscopic	20:32	arg1	analysis					34:41	X-ray photoelectron spectroscopic analysis	0:41	X-ray photoelectron spectroscopic analysis of rice kernels and flours: Measurement of surface chemical composition.	0:114	X-ray photoelectron spectroscopic analysis of rice kernels and flours: Measurement of surface chemical composition.
27374542	5	26	theme	imaging	924:930	arg1	Microscopy					998:1007	Confocal Laser Scanning Microscopy	974:1007	Confocal Laser Scanning Microscopy	974:1007	High resolution imaging methods (Scanning Electron Microscopy and Confocal Laser Scanning Microscopy) were employed to obtain complementary information about the properties and location of starch, proteins and lipids in rice kernels and flours.
27374542	5	26	theme	imaging	924:930	arg1	Microscopy					959:968	Scanning Electron Microscopy	941:968	Scanning Electron Microscopy	941:968	High resolution imaging methods (Scanning Electron Microscopy and Confocal Laser Scanning Microscopy) were employed to obtain complementary information about the properties and location of starch, proteins and lipids in rice kernels and flours.
27374542	5	26	theme	imaging	924:930	arg1	methods					932:938	High resolution imaging methods	908:938	High resolution imaging methods (Scanning Electron Microscopy and Confocal Laser Scanning Microscopy)	908:1008	High resolution imaging methods (Scanning Electron Microscopy and Confocal Laser Scanning Microscopy) were employed to obtain complementary information about the properties and location of starch, proteins and lipids in rice kernels and flours.
27374542	1	27	theme	spectroscopy	197:208	arg1	ability					166:172	the ability	162:172	the ability of X-ray photoelectron spectroscopy (XPS) to differentiate rice macromolecules	162:251	The objectives of this study were to evaluate the ability of X-ray photoelectron spectroscopy (XPS) to differentiate rice macromolecules and to calculate the surface composition of rice kernels and flours.
27374542	0	28	theme	rice	46:49	arg1	kernels					51:57	rice kernels	46:57	rice kernels	46:57	X-ray photoelectron spectroscopic analysis of rice kernels and flours: Measurement of surface chemical composition.
27374542	3	29	theme	uncooked	701:708	arg1	kernels					715:721	uncooked rice kernels	701:721	uncooked rice kernels	701:721	The results of the study showed that XPS was able to differentiate rice polysaccharides (mainly starch), proteins and lipids in uncooked rice kernels and flours.
27374542	5	30	theme	Laser	983:987	arg1	Microscopy					998:1007	Confocal Laser Scanning Microscopy	974:1007	Confocal Laser Scanning Microscopy	974:1007	High resolution imaging methods (Scanning Electron Microscopy and Confocal Laser Scanning Microscopy) were employed to obtain complementary information about the properties and location of starch, proteins and lipids in rice kernels and flours.
27374542	5	30	theme	Laser	983:987	arg1	methods					932:938	High resolution imaging methods	908:938	High resolution imaging methods (Scanning Electron Microscopy and Confocal Laser Scanning Microscopy)	908:1008	High resolution imaging methods (Scanning Electron Microscopy and Confocal Laser Scanning Microscopy) were employed to obtain complementary information about the properties and location of starch, proteins and lipids in rice kernels and flours.
27374542	2	31	theme	varieties	399:407	arg1	kernels					335:341	uncooked kernels	326:341	uncooked kernels	326:341	The uncooked kernels and flours surface composition of the two selected rice varieties, Thadokkham-11 (TDK11) and Doongara (DG) demonstrated an over-expression of lipids and proteins and an under-expression of starch compared to the bulk composition.
27374542	2	31	theme	varieties	399:407	arg1	composition					362:372	flours surface composition	347:372	flours surface composition	347:372	The uncooked kernels and flours surface composition of the two selected rice varieties, Thadokkham-11 (TDK11) and Doongara (DG) demonstrated an over-expression of lipids and proteins and an under-expression of starch compared to the bulk composition.
27374542	5	32	from	lipids	1118:1123	arg1	kernels					1133:1139	rice kernels	1128:1139	rice kernels	1128:1139	High resolution imaging methods (Scanning Electron Microscopy and Confocal Laser Scanning Microscopy) were employed to obtain complementary information about the properties and location of starch, proteins and lipids in rice kernels and flours.
27374542	5	32	from	lipids	1118:1123	arg1	flours					1145:1150	flours	1145:1150	flours	1145:1150	High resolution imaging methods (Scanning Electron Microscopy and Confocal Laser Scanning Microscopy) were employed to obtain complementary information about the properties and location of starch, proteins and lipids in rice kernels and flours.
27374542	1	33	theme	rice	297:300	arg1	kernels					302:308	rice kernels	297:308	rice kernels	297:308	The objectives of this study were to evaluate the ability of X-ray photoelectron spectroscopy (XPS) to differentiate rice macromolecules and to calculate the surface composition of rice kernels and flours.
27374542	2	34	theme	rice	394:397	arg1	varieties					399:407	the two selected rice varieties	377:407	the two selected rice varieties	377:407	The uncooked kernels and flours surface composition of the two selected rice varieties, Thadokkham-11 (TDK11) and Doongara (DG) demonstrated an over-expression of lipids and proteins and an under-expression of starch compared to the bulk composition.
27374542	2	34	theme	rice	394:397	arg1	Doongara					436:443	Doongara	436:443	Doongara (DG)	436:448	The uncooked kernels and flours surface composition of the two selected rice varieties, Thadokkham-11 (TDK11) and Doongara (DG) demonstrated an over-expression of lipids and proteins and an under-expression of starch compared to the bulk composition.
27374542	2	34	theme	rice	394:397	arg1	Thadokkham-11					410:422	Thadokkham-11	410:422	Thadokkham-11 (TDK11)	410:430	The uncooked kernels and flours surface composition of the two selected rice varieties, Thadokkham-11 (TDK11) and Doongara (DG) demonstrated an over-expression of lipids and proteins and an under-expression of starch compared to the bulk composition.
27374542	1	35	theme	kernels	302:308	arg1	composition					282:292	the surface composition	270:292	the surface composition of rice kernels and flours	270:319	The objectives of this study were to evaluate the ability of X-ray photoelectron spectroscopy (XPS) to differentiate rice macromolecules and to calculate the surface composition of rice kernels and flours.
27374542	2	36	theme	bulk	555:558	arg1	composition					560:570	the bulk composition	551:570	the bulk composition	551:570	The uncooked kernels and flours surface composition of the two selected rice varieties, Thadokkham-11 (TDK11) and Doongara (DG) demonstrated an over-expression of lipids and proteins and an under-expression of starch compared to the bulk composition.
27374542	3	37	dep	mainly	662:667	arg1	starch					669:674	starch	669:674	starch	669:674	The results of the study showed that XPS was able to differentiate rice polysaccharides (mainly starch), proteins and lipids in uncooked rice kernels and flours.
27374542	2	38	theme	proteins	496:503	arg1	over-expression					466:480	an over-expression	463:480	an over-expression of lipids and proteins	463:503	The uncooked kernels and flours surface composition of the two selected rice varieties, Thadokkham-11 (TDK11) and Doongara (DG) demonstrated an over-expression of lipids and proteins and an under-expression of starch compared to the bulk composition.
27374542	2	38	theme	proteins	496:503	arg1	under-expression					512:527	an under-expression	509:527	an under-expression of starch	509:537	The uncooked kernels and flours surface composition of the two selected rice varieties, Thadokkham-11 (TDK11) and Doongara (DG) demonstrated an over-expression of lipids and proteins and an under-expression of starch compared to the bulk composition.
27374542	4	39	from	components	778:787	arg1	samples					804:810	cooked rice samples	792:810	cooked rice samples	792:810	Nevertheless, it was unable to distinguish components in cooked rice samples possibly due to complex interactions between gelatinized starch, denatured proteins and lipids.
27374542	0	40	theme	kernels	51:57	arg1	analysis					34:41	X-ray photoelectron spectroscopic analysis	0:41	X-ray photoelectron spectroscopic analysis of rice kernels and flours: Measurement of surface chemical composition.	0:114	X-ray photoelectron spectroscopic analysis of rice kernels and flours: Measurement of surface chemical composition.
27374542	3	41	theme	rice	640:643	arg1	proteins					678:685	proteins	678:685	proteins	678:685	The results of the study showed that XPS was able to differentiate rice polysaccharides (mainly starch), proteins and lipids in uncooked rice kernels and flours.
27374542	3	41	theme	rice	640:643	arg1	lipids					691:696	lipids	691:696	lipids	691:696	The results of the study showed that XPS was able to differentiate rice polysaccharides (mainly starch), proteins and lipids in uncooked rice kernels and flours.
27374542	3	41	theme	rice	640:643	arg1	polysaccharides					645:659	rice polysaccharides	640:659	rice polysaccharides (mainly starch)	640:675	The results of the study showed that XPS was able to differentiate rice polysaccharides (mainly starch), proteins and lipids in uncooked rice kernels and flours.
27374542	2	42	theme	uncooked	326:333	arg1	kernels					335:341	uncooked kernels	326:341	uncooked kernels	326:341	The uncooked kernels and flours surface composition of the two selected rice varieties, Thadokkham-11 (TDK11) and Doongara (DG) demonstrated an over-expression of lipids and proteins and an under-expression of starch compared to the bulk composition.
27374542	1	43	theme	flours	314:319	arg1	composition					282:292	the surface composition	270:292	the surface composition of rice kernels and flours	270:319	The objectives of this study were to evaluate the ability of X-ray photoelectron spectroscopy (XPS) to differentiate rice macromolecules and to calculate the surface composition of rice kernels and flours.
27374542	3	44	theme	study	592:596	arg1	results					577:583	The results	573:583	The results of the study	573:596	The results of the study showed that XPS was able to differentiate rice polysaccharides (mainly starch), proteins and lipids in uncooked rice kernels and flours.
27374542	0	45	dep	analysis	34:41	arg1	Measurement					71:81	Measurement	71:81	X-ray photoelectron spectroscopic analysis of rice kernels and flours: Measurement of surface chemical composition.	0:114	X-ray photoelectron spectroscopic analysis of rice kernels and flours: Measurement of surface chemical composition.
27374542	5	46	dep	methods	932:938	arg1	Microscopy					998:1007	Confocal Laser Scanning Microscopy	974:1007	Confocal Laser Scanning Microscopy	974:1007	High resolution imaging methods (Scanning Electron Microscopy and Confocal Laser Scanning Microscopy) were employed to obtain complementary information about the properties and location of starch, proteins and lipids in rice kernels and flours.
27374542	5	46	dep	methods	932:938	arg1	Microscopy					959:968	Scanning Electron Microscopy	941:968	Scanning Electron Microscopy	941:968	High resolution imaging methods (Scanning Electron Microscopy and Confocal Laser Scanning Microscopy) were employed to obtain complementary information about the properties and location of starch, proteins and lipids in rice kernels and flours.
27374542	5	46	dep	methods	932:938	arg1	methods					932:938	High resolution imaging methods	908:938	High resolution imaging methods (Scanning Electron Microscopy and Confocal Laser Scanning Microscopy)	908:1008	High resolution imaging methods (Scanning Electron Microscopy and Confocal Laser Scanning Microscopy) were employed to obtain complementary information about the properties and location of starch, proteins and lipids in rice kernels and flours.
27374542	5	47	theme	resolution	913:922	arg1	Microscopy					998:1007	Confocal Laser Scanning Microscopy	974:1007	Confocal Laser Scanning Microscopy	974:1007	High resolution imaging methods (Scanning Electron Microscopy and Confocal Laser Scanning Microscopy) were employed to obtain complementary information about the properties and location of starch, proteins and lipids in rice kernels and flours.
27374542	5	47	theme	resolution	913:922	arg1	Microscopy					959:968	Scanning Electron Microscopy	941:968	Scanning Electron Microscopy	941:968	High resolution imaging methods (Scanning Electron Microscopy and Confocal Laser Scanning Microscopy) were employed to obtain complementary information about the properties and location of starch, proteins and lipids in rice kernels and flours.
27374542	5	47	theme	resolution	913:922	arg1	methods					932:938	High resolution imaging methods	908:938	High resolution imaging methods (Scanning Electron Microscopy and Confocal Laser Scanning Microscopy)	908:1008	High resolution imaging methods (Scanning Electron Microscopy and Confocal Laser Scanning Microscopy) were employed to obtain complementary information about the properties and location of starch, proteins and lipids in rice kernels and flours.
27374542	4	48	theme	starch	869:874	arg1	proteins					887:894	gelatinized starch, denatured proteins	857:894	gelatinized starch, denatured proteins	857:894	Nevertheless, it was unable to distinguish components in cooked rice samples possibly due to complex interactions between gelatinized starch, denatured proteins and lipids.
27374542	5	49	theme	Electron	950:957	arg1	Microscopy					959:968	Scanning Electron Microscopy	941:968	Scanning Electron Microscopy	941:968	High resolution imaging methods (Scanning Electron Microscopy and Confocal Laser Scanning Microscopy) were employed to obtain complementary information about the properties and location of starch, proteins and lipids in rice kernels and flours.
27374542	5	49	theme	Electron	950:957	arg1	methods					932:938	High resolution imaging methods	908:938	High resolution imaging methods (Scanning Electron Microscopy and Confocal Laser Scanning Microscopy)	908:1008	High resolution imaging methods (Scanning Electron Microscopy and Confocal Laser Scanning Microscopy) were employed to obtain complementary information about the properties and location of starch, proteins and lipids in rice kernels and flours.
27374542	5	50	dep	properties	1070:1079	arg1	the					1066:1068	the	1066:1068	the	1066:1068	High resolution imaging methods (Scanning Electron Microscopy and Confocal Laser Scanning Microscopy) were employed to obtain complementary information about the properties and location of starch, proteins and lipids in rice kernels and flours.
27374542	5	51	theme	rice	1128:1131	arg1	kernels					1133:1139	rice kernels	1128:1139	rice kernels	1128:1139	High resolution imaging methods (Scanning Electron Microscopy and Confocal Laser Scanning Microscopy) were employed to obtain complementary information about the properties and location of starch, proteins and lipids in rice kernels and flours.
27374542	5	52	theme	complementary	1034:1046	arg1	information					1048:1058	complementary information	1034:1058	complementary information about the properties and location of starch, proteins and lipids in rice kernels and flours	1034:1150	High resolution imaging methods (Scanning Electron Microscopy and Confocal Laser Scanning Microscopy) were employed to obtain complementary information about the properties and location of starch, proteins and lipids in rice kernels and flours.
27374542	5	53	theme	Confocal	974:981	arg1	Microscopy					998:1007	Confocal Laser Scanning Microscopy	974:1007	Confocal Laser Scanning Microscopy	974:1007	High resolution imaging methods (Scanning Electron Microscopy and Confocal Laser Scanning Microscopy) were employed to obtain complementary information about the properties and location of starch, proteins and lipids in rice kernels and flours.
27374542	5	53	theme	Confocal	974:981	arg1	methods					932:938	High resolution imaging methods	908:938	High resolution imaging methods (Scanning Electron Microscopy and Confocal Laser Scanning Microscopy)	908:1008	High resolution imaging methods (Scanning Electron Microscopy and Confocal Laser Scanning Microscopy) were employed to obtain complementary information about the properties and location of starch, proteins and lipids in rice kernels and flours.
27374542	1	54	theme	study	139:143	arg1	objectives					120:129	The objectives	116:129	The objectives of this study	116:143	The objectives of this study were to evaluate the ability of X-ray photoelectron spectroscopy (XPS) to differentiate rice macromolecules and to calculate the surface composition of rice kernels and flours.
27374542	0	55	theme	flours	63:68	arg1	analysis					34:41	X-ray photoelectron spectroscopic analysis	0:41	X-ray photoelectron spectroscopic analysis of rice kernels and flours: Measurement of surface chemical composition.	0:114	X-ray photoelectron spectroscopic analysis of rice kernels and flours: Measurement of surface chemical composition.
27374542	4	56	dep	starch	869:874	arg1	denatured					877:885	denatured	877:885	denatured	877:885	Nevertheless, it was unable to distinguish components in cooked rice samples possibly due to complex interactions between gelatinized starch, denatured proteins and lipids.
27374542	3	57	from	lipids	691:696	arg1	flours					727:732	flours	727:732	flours	727:732	The results of the study showed that XPS was able to differentiate rice polysaccharides (mainly starch), proteins and lipids in uncooked rice kernels and flours.
27374542	3	57	from	lipids	691:696	arg1	kernels					715:721	uncooked rice kernels	701:721	uncooked rice kernels	701:721	The results of the study showed that XPS was able to differentiate rice polysaccharides (mainly starch), proteins and lipids in uncooked rice kernels and flours.
27374542	2	58	theme	surface	354:360	arg1	composition					362:372	flours surface composition	347:372	flours surface composition	347:372	The uncooked kernels and flours surface composition of the two selected rice varieties, Thadokkham-11 (TDK11) and Doongara (DG) demonstrated an over-expression of lipids and proteins and an under-expression of starch compared to the bulk composition.
27374542	1	59	theme	rice	233:236	arg1	macromolecules					238:251	rice macromolecules	233:251	rice macromolecules	233:251	The objectives of this study were to evaluate the ability of X-ray photoelectron spectroscopy (XPS) to differentiate rice macromolecules and to calculate the surface composition of rice kernels and flours.
28950170	0	0	theme	nanoparticle	81:92	arg1	composites					94:103	gold nanoparticle composites	76:103	gold nanoparticle composites	76:103	Facile synthesis, structure, biocompatibility and antimicrobial property of gold nanoparticle composites from cellulose and keratin.
28950170	5	1	theme	Encapsulating	745:757	arg1	composite					781:789	Encapsulating Au0NPs into [CEL+KER] composite	745:789	Encapsulating Au0NPs into [CEL+KER] composite	745:789	Encapsulating Au0NPs into [CEL+KER] composite made the composite fully biocompatible and their bactericidal capabilities were increased by the antibacterial activity of Au0NPs.
28950170	2	2	used	used	395:398	arg2	liquids					286:292	Two ionic liquids	276:292	Two ionic liquids	276:292	Two ionic liquids, butylmethylimmidazolium chloride and ethylmethylimmidazolium bis(trifluoromethylsulfonyl)imide were used to dissolve CEL, KER and HAuCl4.
28950170	2	2	used	used	395:398	arg2	imide					384:388	ethylmethylimmidazolium bis(trifluoromethylsulfonyl)imide	332:388	ethylmethylimmidazolium bis(trifluoromethylsulfonyl)imide	332:388	Two ionic liquids, butylmethylimmidazolium chloride and ethylmethylimmidazolium bis(trifluoromethylsulfonyl)imide were used to dissolve CEL, KER and HAuCl4.
28950170	2	2	used	used	395:398	arg2	chloride					319:326	butylmethylimmidazolium chloride	295:326	butylmethylimmidazolium chloride	295:326	Two ionic liquids, butylmethylimmidazolium chloride and ethylmethylimmidazolium bis(trifluoromethylsulfonyl)imide were used to dissolve CEL, KER and HAuCl4.
28950170	2	3	theme	bis	356:358	arg1	imide					384:388	ethylmethylimmidazolium bis(trifluoromethylsulfonyl)imide	332:388	ethylmethylimmidazolium bis(trifluoromethylsulfonyl)imide	332:388	Two ionic liquids, butylmethylimmidazolium chloride and ethylmethylimmidazolium bis(trifluoromethylsulfonyl)imide were used to dissolve CEL, KER and HAuCl4.
28950170	2	3	theme	bis	356:358	arg1	liquids					286:292	Two ionic liquids	276:292	Two ionic liquids	276:292	Two ionic liquids, butylmethylimmidazolium chloride and ethylmethylimmidazolium bis(trifluoromethylsulfonyl)imide were used to dissolve CEL, KER and HAuCl4.
28950170	1	4	theme	wool	235:238	arg1	keratin					240:246	wool keratin	235:246	wool keratin (KER)	235:252	A novel, one-pot method was developed to synthesize gold nanoparticle composite from cellulose (CEL), wool keratin (KER) and chloroauric acid.
28950170	1	4	theme	wool	235:238	arg1	KER					249:251	KER	249:251	KER	249:251	A novel, one-pot method was developed to synthesize gold nanoparticle composite from cellulose (CEL), wool keratin (KER) and chloroauric acid.
28950170	0	5	theme	gold	76:79	arg1	composites					94:103	gold nanoparticle composites	76:103	gold nanoparticle composites	76:103	Facile synthesis, structure, biocompatibility and antimicrobial property of gold nanoparticle composites from cellulose and keratin.
28950170	8	6	theme	required	1526:1533	arg1	properties					1535:1544	all required properties	1522:1544	all required properties for successful use as dressing to treat chronic ulcerous infected wounds	1522:1617	These results together with our previous finding that [CEL+KER] composites can be used for controlled delivery of drugs clearly indicate that the [CEL+KER+Au0NPs] composites possess all required properties for successful use as dressing to treat chronic ulcerous infected wounds.
28950170	6	7	from	reduction	995:1003	arg1	growth					1008:1013	growth	1008:1013	growth of antibiotic resistant bacteria such as vancomycin resistant Enterococcus faecalis and methicillin resistant Staphylococcus aureus	1008:1145	Specifically, the [CEL+KER+Au0NPs] composite exhibited up to 97% and 98% reduction in growth of antibiotic resistant bacteria such as vancomycin resistant Enterococcus faecalis and methicillin resistant Staphylococcus aureus, and was not cytotoxic to human fibroblasts.
28950170	2	8	theme	ethylmethylimmidazolium	332:354	arg1	imide					384:388	ethylmethylimmidazolium bis(trifluoromethylsulfonyl)imide	332:388	ethylmethylimmidazolium bis(trifluoromethylsulfonyl)imide	332:388	Two ionic liquids, butylmethylimmidazolium chloride and ethylmethylimmidazolium bis(trifluoromethylsulfonyl)imide were used to dissolve CEL, KER and HAuCl4.
28950170	2	8	theme	ethylmethylimmidazolium	332:354	arg1	liquids					286:292	Two ionic liquids	276:292	Two ionic liquids	276:292	Two ionic liquids, butylmethylimmidazolium chloride and ethylmethylimmidazolium bis(trifluoromethylsulfonyl)imide were used to dissolve CEL, KER and HAuCl4.
28950170	6	9	theme	98	991:992	arg1	%					993:993	%	993:993	%	993:993	Specifically, the [CEL+KER+Au0NPs] composite exhibited up to 97% and 98% reduction in growth of antibiotic resistant bacteria such as vancomycin resistant Enterococcus faecalis and methicillin resistant Staphylococcus aureus, and was not cytotoxic to human fibroblasts.
28950170	8	10	theme	chronic	1586:1592	arg1	wounds					1612:1617	chronic ulcerous infected wounds	1586:1617	chronic ulcerous infected wounds	1586:1617	These results together with our previous finding that [CEL+KER] composites can be used for controlled delivery of drugs clearly indicate that the [CEL+KER+Au0NPs] composites possess all required properties for successful use as dressing to treat chronic ulcerous infected wounds.
28950170	7	11	contain	possess	1230:1236	arg1	composite					1208:1216	[CEL+KER] composite	1198:1216	[CEL+KER] composite	1198:1216	While [CEL+KER] composite is known to possess some antibacterial activity, the enhanced antibacterial observed here was due solely to added Au0NPs.
28950170	7	11	contain	possess	1230:1236	arg2	activity					1257:1264	some antibacterial activity	1238:1264	some antibacterial activity	1238:1264	While [CEL+KER] composite is known to possess some antibacterial activity, the enhanced antibacterial observed here was due solely to added Au0NPs.
28950170	0	12	theme	composites	94:103	arg1	biocompatibility					29:44	biocompatibility	29:44	biocompatibility	29:44	Facile synthesis, structure, biocompatibility and antimicrobial property of gold nanoparticle composites from cellulose and keratin.
28950170	0	12	theme	composites	94:103	arg1	property					64:71	antimicrobial property	50:71	antimicrobial property	50:71	Facile synthesis, structure, biocompatibility and antimicrobial property of gold nanoparticle composites from cellulose and keratin.
28950170	0	12	theme	composites	94:103	arg1	structure					18:26	structure	18:26	structure	18:26	Facile synthesis, structure, biocompatibility and antimicrobial property of gold nanoparticle composites from cellulose and keratin.
28950170	0	12	theme	composites	94:103	arg1	synthesis					7:15	Facile synthesis	0:15	Facile synthesis	0:15	Facile synthesis, structure, biocompatibility and antimicrobial property of gold nanoparticle composites from cellulose and keratin.
28950170	1	13	dep	novel	135:139	arg1	one-pot					142:148	one-pot	142:148	one-pot	142:148	A novel, one-pot method was developed to synthesize gold nanoparticle composite from cellulose (CEL), wool keratin (KER) and chloroauric acid.
28950170	6	14	theme	%	985:985	arg1	reduction					995:1003	up to 97% and 98% reduction	977:1003	up to 97% and 98% reduction in growth of antibiotic resistant bacteria such as vancomycin resistant Enterococcus faecalis and methicillin resistant Staphylococcus aureus	977:1145	Specifically, the [CEL+KER+Au0NPs] composite exhibited up to 97% and 98% reduction in growth of antibiotic resistant bacteria such as vancomycin resistant Enterococcus faecalis and methicillin resistant Staphylococcus aureus, and was not cytotoxic to human fibroblasts.
28950170	7	15	theme	[CEL+KER	1198:1205	arg1	composite					1208:1216	[CEL+KER] composite	1198:1216	[CEL+KER] composite	1198:1216	While [CEL+KER] composite is known to possess some antibacterial activity, the enhanced antibacterial observed here was due solely to added Au0NPs.
28950170	4	16	theme	Spectroscopy	593:604	arg1	results					618:624	Spectroscopy and imaging results	593:624	results	618:624	Spectroscopy and imaging results indicate that CEL and KER remained chemically intact and were homogeneously distributed in the composites with Au0NPs.
28950170	3	17	dep	nm	552:553	arg1	5.5±1					545:549	5.5±1	545:549	5.5±1	545:549	X-ray diffraction and X-ray photoelectron results show that Au3+ was completely reduced to Au0NPs with size of (5.5±1) nm directly in the composite with NaBH4.
28950170	6	18	theme	bacteria	1039:1046	arg1	growth					1008:1013	growth	1008:1013	growth of antibiotic resistant bacteria such as vancomycin resistant Enterococcus faecalis and methicillin resistant Staphylococcus aureus	1008:1145	Specifically, the [CEL+KER+Au0NPs] composite exhibited up to 97% and 98% reduction in growth of antibiotic resistant bacteria such as vancomycin resistant Enterococcus faecalis and methicillin resistant Staphylococcus aureus, and was not cytotoxic to human fibroblasts.
28950170	8	19	used	used	1422:1425	arg2	finding					1381:1387	our previous finding	1368:1387	our previous finding that [CEL+KER] composites	1368:1413	These results together with our previous finding that [CEL+KER] composites can be used for controlled delivery of drugs clearly indicate that the [CEL+KER+Au0NPs] composites possess all required properties for successful use as dressing to treat chronic ulcerous infected wounds.
28950170	6	20	theme	human	1173:1177	arg1	fibroblasts					1179:1189	human fibroblasts	1173:1189	human fibroblasts	1173:1189	Specifically, the [CEL+KER+Au0NPs] composite exhibited up to 97% and 98% reduction in growth of antibiotic resistant bacteria such as vancomycin resistant Enterococcus faecalis and methicillin resistant Staphylococcus aureus, and was not cytotoxic to human fibroblasts.
28950170	6	21	dep	%	985:985	arg1	97					983:984	97	983:984	97	983:984	Specifically, the [CEL+KER+Au0NPs] composite exhibited up to 97% and 98% reduction in growth of antibiotic resistant bacteria such as vancomycin resistant Enterococcus faecalis and methicillin resistant Staphylococcus aureus, and was not cytotoxic to human fibroblasts.
28950170	5	22	theme	Au0NPs	759:764	arg1	composite					781:789	Encapsulating Au0NPs into [CEL+KER] composite	745:789	Encapsulating Au0NPs into [CEL+KER] composite	745:789	Encapsulating Au0NPs into [CEL+KER] composite made the composite fully biocompatible and their bactericidal capabilities were increased by the antibacterial activity of Au0NPs.
28950170	7	23	theme	added	1326:1330	arg1	Au0NPs					1332:1337	added Au0NPs	1326:1337	added Au0NPs	1326:1337	While [CEL+KER] composite is known to possess some antibacterial activity, the enhanced antibacterial observed here was due solely to added Au0NPs.
28950170	0	24	theme	Facile	0:5	arg1	synthesis					7:15	Facile synthesis	0:15	Facile synthesis	0:15	Facile synthesis, structure, biocompatibility and antimicrobial property of gold nanoparticle composites from cellulose and keratin.
28950170	8	25	theme	[CEL+KER+Au0NPs	1486:1500	arg1	composites					1503:1512	the [CEL+KER+Au0NPs] composites	1482:1512	the [CEL+KER+Au0NPs] composites	1482:1512	These results together with our previous finding that [CEL+KER] composites can be used for controlled delivery of drugs clearly indicate that the [CEL+KER+Au0NPs] composites possess all required properties for successful use as dressing to treat chronic ulcerous infected wounds.
28950170	2	26	theme	ionic	280:284	arg1	chloride					319:326	butylmethylimmidazolium chloride	295:326	butylmethylimmidazolium chloride	295:326	Two ionic liquids, butylmethylimmidazolium chloride and ethylmethylimmidazolium bis(trifluoromethylsulfonyl)imide were used to dissolve CEL, KER and HAuCl4.
28950170	2	26	theme	ionic	280:284	arg1	imide					384:388	ethylmethylimmidazolium bis(trifluoromethylsulfonyl)imide	332:388	ethylmethylimmidazolium bis(trifluoromethylsulfonyl)imide	332:388	Two ionic liquids, butylmethylimmidazolium chloride and ethylmethylimmidazolium bis(trifluoromethylsulfonyl)imide were used to dissolve CEL, KER and HAuCl4.
28950170	2	26	theme	ionic	280:284	arg1	liquids					286:292	Two ionic liquids	276:292	Two ionic liquids	276:292	Two ionic liquids, butylmethylimmidazolium chloride and ethylmethylimmidazolium bis(trifluoromethylsulfonyl)imide were used to dissolve CEL, KER and HAuCl4.
28950170	6	27	theme	%	993:993	arg1	reduction					995:1003	up to 97% and 98% reduction	977:1003	up to 97% and 98% reduction in growth of antibiotic resistant bacteria such as vancomycin resistant Enterococcus faecalis and methicillin resistant Staphylococcus aureus	977:1145	Specifically, the [CEL+KER+Au0NPs] composite exhibited up to 97% and 98% reduction in growth of antibiotic resistant bacteria such as vancomycin resistant Enterococcus faecalis and methicillin resistant Staphylococcus aureus, and was not cytotoxic to human fibroblasts.
28950170	1	28	theme	gold	185:188	arg1	composite					203:211	gold nanoparticle composite	185:211	gold nanoparticle composite	185:211	A novel, one-pot method was developed to synthesize gold nanoparticle composite from cellulose (CEL), wool keratin (KER) and chloroauric acid.
28950170	6	29	theme	resistant	1029:1037	arg1	bacteria					1039:1046	antibiotic resistant bacteria	1018:1046	antibiotic resistant bacteria such as vancomycin resistant Enterococcus faecalis and methicillin resistant Staphylococcus aureus	1018:1145	Specifically, the [CEL+KER+Au0NPs] composite exhibited up to 97% and 98% reduction in growth of antibiotic resistant bacteria such as vancomycin resistant Enterococcus faecalis and methicillin resistant Staphylococcus aureus, and was not cytotoxic to human fibroblasts.
28950170	6	29	theme	resistant	1029:1037	arg1	faecalis					1090:1097	Enterococcus faecalis	1077:1097	Enterococcus faecalis	1077:1097	Specifically, the [CEL+KER+Au0NPs] composite exhibited up to 97% and 98% reduction in growth of antibiotic resistant bacteria such as vancomycin resistant Enterococcus faecalis and methicillin resistant Staphylococcus aureus, and was not cytotoxic to human fibroblasts.
28950170	6	29	theme	resistant	1029:1037	arg1	resistant					1115:1123	resistant	1115:1123	resistant	1115:1123	Specifically, the [CEL+KER+Au0NPs] composite exhibited up to 97% and 98% reduction in growth of antibiotic resistant bacteria such as vancomycin resistant Enterococcus faecalis and methicillin resistant Staphylococcus aureus, and was not cytotoxic to human fibroblasts.
28950170	1	30	theme	nanoparticle	190:201	arg1	composite					203:211	gold nanoparticle composite	185:211	gold nanoparticle composite	185:211	A novel, one-pot method was developed to synthesize gold nanoparticle composite from cellulose (CEL), wool keratin (KER) and chloroauric acid.
28950170	8	31	theme	previous	1372:1379	arg1	finding					1381:1387	our previous finding	1368:1387	our previous finding that [CEL+KER] composites	1368:1413	These results together with our previous finding that [CEL+KER] composites can be used for controlled delivery of drugs clearly indicate that the [CEL+KER+Au0NPs] composites possess all required properties for successful use as dressing to treat chronic ulcerous infected wounds.
28950170	1	32	theme	chloroauric	258:268	arg1	acid					270:273	chloroauric acid	258:273	chloroauric acid	258:273	A novel, one-pot method was developed to synthesize gold nanoparticle composite from cellulose (CEL), wool keratin (KER) and chloroauric acid.
28950170	8	33	theme	drugs	1454:1458	arg1	delivery					1442:1449	controlled delivery	1431:1449	controlled delivery of drugs	1431:1458	These results together with our previous finding that [CEL+KER] composites can be used for controlled delivery of drugs clearly indicate that the [CEL+KER+Au0NPs] composites possess all required properties for successful use as dressing to treat chronic ulcerous infected wounds.
28950170	3	34	theme	X-ray	455:459	arg1	photoelectron					461:473	X-ray photoelectron	455:473	X-ray photoelectron	455:473	X-ray diffraction and X-ray photoelectron results show that Au3+ was completely reduced to Au0NPs with size of (5.5±1) nm directly in the composite with NaBH4.
28950170	5	35	theme	into	766:769	arg1	composite					781:789	Encapsulating Au0NPs into [CEL+KER] composite	745:789	Encapsulating Au0NPs into [CEL+KER] composite	745:789	Encapsulating Au0NPs into [CEL+KER] composite made the composite fully biocompatible and their bactericidal capabilities were increased by the antibacterial activity of Au0NPs.
28950170	3	36	dep	NaBH4	586:590	arg1	the					567:569	the	567:569	the	567:569	X-ray diffraction and X-ray photoelectron results show that Au3+ was completely reduced to Au0NPs with size of (5.5±1) nm directly in the composite with NaBH4.
28950170	3	36	dep	NaBH4	586:590	arg1	composite					571:579	composite	571:579	composite	571:579	X-ray diffraction and X-ray photoelectron results show that Au3+ was completely reduced to Au0NPs with size of (5.5±1) nm directly in the composite with NaBH4.
28950170	8	37	contain	possess	1514:1520	arg1	composites					1503:1512	the [CEL+KER+Au0NPs] composites	1482:1512	the [CEL+KER+Au0NPs] composites	1482:1512	These results together with our previous finding that [CEL+KER] composites can be used for controlled delivery of drugs clearly indicate that the [CEL+KER+Au0NPs] composites possess all required properties for successful use as dressing to treat chronic ulcerous infected wounds.
28950170	8	37	contain	possess	1514:1520	arg2	properties					1535:1544	all required properties	1522:1544	all required properties for successful use as dressing to treat chronic ulcerous infected wounds	1522:1617	These results together with our previous finding that [CEL+KER] composites can be used for controlled delivery of drugs clearly indicate that the [CEL+KER+Au0NPs] composites possess all required properties for successful use as dressing to treat chronic ulcerous infected wounds.
28950170	3	38	theme	X-ray	433:437	arg1	diffraction					439:449	X-ray diffraction	433:449	X-ray diffraction	433:449	X-ray diffraction and X-ray photoelectron results show that Au3+ was completely reduced to Au0NPs with size of (5.5±1) nm directly in the composite with NaBH4.
28950170	3	39	with	Au0NPs	524:529	arg1	size					536:539	size	536:539	size	536:539	X-ray diffraction and X-ray photoelectron results show that Au3+ was completely reduced to Au0NPs with size of (5.5±1) nm directly in the composite with NaBH4.
28950170	8	40	theme	ulcerous	1594:1601	arg1	wounds					1612:1617	chronic ulcerous infected wounds	1586:1617	chronic ulcerous infected wounds	1586:1617	These results together with our previous finding that [CEL+KER] composites can be used for controlled delivery of drugs clearly indicate that the [CEL+KER+Au0NPs] composites possess all required properties for successful use as dressing to treat chronic ulcerous infected wounds.
28950170	5	41	theme	[CEL+KER	771:778	arg1	composite					781:789	Encapsulating Au0NPs into [CEL+KER] composite	745:789	Encapsulating Au0NPs into [CEL+KER] composite	745:789	Encapsulating Au0NPs into [CEL+KER] composite made the composite fully biocompatible and their bactericidal capabilities were increased by the antibacterial activity of Au0NPs.
28950170	6	42	dep	faecalis	1090:1097	arg1	aureus					1140:1145	Staphylococcus aureus	1125:1145	Staphylococcus aureus	1125:1145	Specifically, the [CEL+KER+Au0NPs] composite exhibited up to 97% and 98% reduction in growth of antibiotic resistant bacteria such as vancomycin resistant Enterococcus faecalis and methicillin resistant Staphylococcus aureus, and was not cytotoxic to human fibroblasts.
28950170	5	43	theme	bactericidal	840:851	arg1	capabilities					853:864	their bactericidal capabilities	834:864	their bactericidal capabilities	834:864	Encapsulating Au0NPs into [CEL+KER] composite made the composite fully biocompatible and their bactericidal capabilities were increased by the antibacterial activity of Au0NPs.
28950170	3	44	theme	diffraction	439:449	arg1	results					475:481	X-ray diffraction and X-ray photoelectron results	433:481	X-ray diffraction and X-ray photoelectron results	433:481	X-ray diffraction and X-ray photoelectron results show that Au3+ was completely reduced to Au0NPs with size of (5.5±1) nm directly in the composite with NaBH4.
28950170	0	45	theme	antimicrobial	50:62	arg1	property					64:71	antimicrobial property	50:71	antimicrobial property	50:71	Facile synthesis, structure, biocompatibility and antimicrobial property of gold nanoparticle composites from cellulose and keratin.
28950170	4	46	with	composites	721:730	arg1	Au0NPs					737:742	Au0NPs	737:742	Au0NPs	737:742	Spectroscopy and imaging results indicate that CEL and KER remained chemically intact and were homogeneously distributed in the composites with Au0NPs.
28950170	6	47	theme	antibiotic	1018:1027	arg1	bacteria					1039:1046	antibiotic resistant bacteria	1018:1046	antibiotic resistant bacteria such as vancomycin resistant Enterococcus faecalis and methicillin resistant Staphylococcus aureus	1018:1145	Specifically, the [CEL+KER+Au0NPs] composite exhibited up to 97% and 98% reduction in growth of antibiotic resistant bacteria such as vancomycin resistant Enterococcus faecalis and methicillin resistant Staphylococcus aureus, and was not cytotoxic to human fibroblasts.
28950170	6	47	theme	antibiotic	1018:1027	arg1	faecalis					1090:1097	Enterococcus faecalis	1077:1097	Enterococcus faecalis	1077:1097	Specifically, the [CEL+KER+Au0NPs] composite exhibited up to 97% and 98% reduction in growth of antibiotic resistant bacteria such as vancomycin resistant Enterococcus faecalis and methicillin resistant Staphylococcus aureus, and was not cytotoxic to human fibroblasts.
28950170	6	47	theme	antibiotic	1018:1027	arg1	resistant					1115:1123	resistant	1115:1123	resistant	1115:1123	Specifically, the [CEL+KER+Au0NPs] composite exhibited up to 97% and 98% reduction in growth of antibiotic resistant bacteria such as vancomycin resistant Enterococcus faecalis and methicillin resistant Staphylococcus aureus, and was not cytotoxic to human fibroblasts.
28950170	3	48	theme	nm	552:553	arg1	Au0NPs					524:529	Au0NPs	524:529	Au0NPs with size of (5.5±1) nm	524:553	X-ray diffraction and X-ray photoelectron results show that Au3+ was completely reduced to Au0NPs with size of (5.5±1) nm directly in the composite with NaBH4.
28950170	5	49	theme	Au0NPs	914:919	arg1	activity					902:909	the antibacterial activity	884:909	the antibacterial activity of Au0NPs	884:919	Encapsulating Au0NPs into [CEL+KER] composite made the composite fully biocompatible and their bactericidal capabilities were increased by the antibacterial activity of Au0NPs.
28950170	6	50	theme	[CEL+KER+Au0NPs	940:954	arg1	cytotoxic					1160:1168	cytotoxic	1160:1168	cytotoxic	1160:1168	Specifically, the [CEL+KER+Au0NPs] composite exhibited up to 97% and 98% reduction in growth of antibiotic resistant bacteria such as vancomycin resistant Enterococcus faecalis and methicillin resistant Staphylococcus aureus, and was not cytotoxic to human fibroblasts.
28950170	6	50	theme	[CEL+KER+Au0NPs	940:954	arg1	composite					957:965	the [CEL+KER+Au0NPs] composite	936:965	the [CEL+KER+Au0NPs] composite	936:965	Specifically, the [CEL+KER+Au0NPs] composite exhibited up to 97% and 98% reduction in growth of antibiotic resistant bacteria such as vancomycin resistant Enterococcus faecalis and methicillin resistant Staphylococcus aureus, and was not cytotoxic to human fibroblasts.
28950170	8	51	dep	together	1354:1361	arg1	used					1422:1425	used	1422:1425	can be used for controlled delivery of drugs	1415:1458	These results together with our previous finding that [CEL+KER] composites can be used for controlled delivery of drugs clearly indicate that the [CEL+KER+Au0NPs] composites possess all required properties for successful use as dressing to treat chronic ulcerous infected wounds.
28950170	8	52	theme	successful	1550:1559	arg1	use					1561:1563	successful use	1550:1563	successful use as dressing to treat chronic ulcerous infected wounds	1550:1617	These results together with our previous finding that [CEL+KER] composites can be used for controlled delivery of drugs clearly indicate that the [CEL+KER+Au0NPs] composites possess all required properties for successful use as dressing to treat chronic ulcerous infected wounds.
28950170	8	53	theme	infected	1603:1610	arg1	wounds					1612:1617	chronic ulcerous infected wounds	1586:1617	chronic ulcerous infected wounds	1586:1617	These results together with our previous finding that [CEL+KER] composites can be used for controlled delivery of drugs clearly indicate that the [CEL+KER+Au0NPs] composites possess all required properties for successful use as dressing to treat chronic ulcerous infected wounds.
28950170	0	54	from	cellulose	110:118	arg1	biocompatibility					29:44	biocompatibility	29:44	biocompatibility	29:44	Facile synthesis, structure, biocompatibility and antimicrobial property of gold nanoparticle composites from cellulose and keratin.
28950170	0	54	from	cellulose	110:118	arg1	property					64:71	antimicrobial property	50:71	antimicrobial property	50:71	Facile synthesis, structure, biocompatibility and antimicrobial property of gold nanoparticle composites from cellulose and keratin.
28950170	0	54	from	cellulose	110:118	arg1	structure					18:26	structure	18:26	structure	18:26	Facile synthesis, structure, biocompatibility and antimicrobial property of gold nanoparticle composites from cellulose and keratin.
28950170	0	54	from	cellulose	110:118	arg1	synthesis					7:15	Facile synthesis	0:15	Facile synthesis	0:15	Facile synthesis, structure, biocompatibility and antimicrobial property of gold nanoparticle composites from cellulose and keratin.
28950170	2	55	theme	butylmethylimmidazolium	295:317	arg1	chloride					319:326	butylmethylimmidazolium chloride	295:326	butylmethylimmidazolium chloride	295:326	Two ionic liquids, butylmethylimmidazolium chloride and ethylmethylimmidazolium bis(trifluoromethylsulfonyl)imide were used to dissolve CEL, KER and HAuCl4.
28950170	2	55	theme	butylmethylimmidazolium	295:317	arg1	liquids					286:292	Two ionic liquids	276:292	Two ionic liquids	276:292	Two ionic liquids, butylmethylimmidazolium chloride and ethylmethylimmidazolium bis(trifluoromethylsulfonyl)imide were used to dissolve CEL, KER and HAuCl4.
28950170	8	56	theme	[CEL+KER	1394:1401	arg1	composites					1404:1413	[CEL+KER] composites	1394:1413	[CEL+KER] composites	1394:1413	These results together with our previous finding that [CEL+KER] composites can be used for controlled delivery of drugs clearly indicate that the [CEL+KER+Au0NPs] composites possess all required properties for successful use as dressing to treat chronic ulcerous infected wounds.
28950170	6	57	dep	97	983:984	arg1	to					980:981	to	980:981	to	980:981	Specifically, the [CEL+KER+Au0NPs] composite exhibited up to 97% and 98% reduction in growth of antibiotic resistant bacteria such as vancomycin resistant Enterococcus faecalis and methicillin resistant Staphylococcus aureus, and was not cytotoxic to human fibroblasts.
28950170	1	58	theme	novel	135:139	arg1	method					150:155	A novel, one-pot method	133:155	A novel, one-pot method	133:155	A novel, one-pot method was developed to synthesize gold nanoparticle composite from cellulose (CEL), wool keratin (KER) and chloroauric acid.
28950170	3	59	theme	photoelectron	461:473	arg1	results					475:481	X-ray diffraction and X-ray photoelectron results	433:481	X-ray diffraction and X-ray photoelectron results	433:481	X-ray diffraction and X-ray photoelectron results show that Au3+ was completely reduced to Au0NPs with size of (5.5±1) nm directly in the composite with NaBH4.
28950170	4	60	theme	imaging	610:616	arg1	results					618:624	Spectroscopy and imaging results	593:624	results	618:624	Spectroscopy and imaging results indicate that CEL and KER remained chemically intact and were homogeneously distributed in the composites with Au0NPs.
28950170	5	61	theme	biocompatible	816:828	arg1	composite					800:808	the composite	796:808	the composite fully biocompatible	796:828	Encapsulating Au0NPs into [CEL+KER] composite made the composite fully biocompatible and their bactericidal capabilities were increased by the antibacterial activity of Au0NPs.
28950170	2	62	theme	trifluoromethylsulfonyl	360:382	arg1	imide					384:388	ethylmethylimmidazolium bis(trifluoromethylsulfonyl)imide	332:388	ethylmethylimmidazolium bis(trifluoromethylsulfonyl)imide	332:388	Two ionic liquids, butylmethylimmidazolium chloride and ethylmethylimmidazolium bis(trifluoromethylsulfonyl)imide were used to dissolve CEL, KER and HAuCl4.
28950170	2	62	theme	trifluoromethylsulfonyl	360:382	arg1	liquids					286:292	Two ionic liquids	276:292	Two ionic liquids	276:292	Two ionic liquids, butylmethylimmidazolium chloride and ethylmethylimmidazolium bis(trifluoromethylsulfonyl)imide were used to dissolve CEL, KER and HAuCl4.
28950170	8	63	theme	controlled	1431:1440	arg1	delivery					1442:1449	controlled delivery	1431:1449	controlled delivery of drugs	1431:1458	These results together with our previous finding that [CEL+KER] composites can be used for controlled delivery of drugs clearly indicate that the [CEL+KER+Au0NPs] composites possess all required properties for successful use as dressing to treat chronic ulcerous infected wounds.
28950170	7	64	theme	enhanced	1271:1278	arg1	antibacterial					1280:1292	the enhanced antibacterial	1267:1292	the enhanced antibacterial observed here	1267:1306	While [CEL+KER] composite is known to possess some antibacterial activity, the enhanced antibacterial observed here was due solely to added Au0NPs.
28950170	7	64	theme	enhanced	1271:1278	arg1	due					1312:1314	due	1312:1314	due	1312:1314	While [CEL+KER] composite is known to possess some antibacterial activity, the enhanced antibacterial observed here was due solely to added Au0NPs.
28950170	5	65	theme	antibacterial	888:900	arg1	activity					902:909	the antibacterial activity	884:909	the antibacterial activity of Au0NPs	884:919	Encapsulating Au0NPs into [CEL+KER] composite made the composite fully biocompatible and their bactericidal capabilities were increased by the antibacterial activity of Au0NPs.
28950170	7	66	theme	antibacterial	1243:1255	arg1	activity					1257:1264	some antibacterial activity	1238:1264	some antibacterial activity	1238:1264	While [CEL+KER] composite is known to possess some antibacterial activity, the enhanced antibacterial observed here was due solely to added Au0NPs.
28950170	0	67	from	keratin	124:130	arg1	biocompatibility					29:44	biocompatibility	29:44	biocompatibility	29:44	Facile synthesis, structure, biocompatibility and antimicrobial property of gold nanoparticle composites from cellulose and keratin.
28950170	0	67	from	keratin	124:130	arg1	property					64:71	antimicrobial property	50:71	antimicrobial property	50:71	Facile synthesis, structure, biocompatibility and antimicrobial property of gold nanoparticle composites from cellulose and keratin.
28950170	0	67	from	keratin	124:130	arg1	structure					18:26	structure	18:26	structure	18:26	Facile synthesis, structure, biocompatibility and antimicrobial property of gold nanoparticle composites from cellulose and keratin.
28950170	0	67	from	keratin	124:130	arg1	synthesis					7:15	Facile synthesis	0:15	Facile synthesis	0:15	Facile synthesis, structure, biocompatibility and antimicrobial property of gold nanoparticle composites from cellulose and keratin.
25659682	5	0	theme	policosanol	588:598	arg1	addition					576:583	Stepwise addition	567:583	Stepwise addition of policosanol and longer time complex formation	567:632	Stepwise addition of policosanol and longer time complex formation increased the dispersible policosanol: about 95% by 30 min interval, 1.0% dextrin solution and 12h complex formation time.
25659682	6	1	with	agent	980:984	arg1	policosanol					991:1001	policosanol	991:1001	policosanol	991:1001	Among the dispersible policosanol (95%), 70% policosanol resided in the precipitates and 25% was in the supernatant, indicating the dextrin behaved as a stabilizer for the dispersed policosanol as well as a complex forming agent with policosanol.
25659682	6	2	theme	forming	972:978	arg1	agent					980:984	a complex forming agent	962:984	the dispersed policosanol as well as a complex forming agent with policosanol	925:1001	Among the dispersible policosanol (95%), 70% policosanol resided in the precipitates and 25% was in the supernatant, indicating the dextrin behaved as a stabilizer for the dispersed policosanol as well as a complex forming agent with policosanol.
25659682	1	3	theme	dextrin	209:215	arg1	solution					146:153	an aqueous solution	135:153	an aqueous solution (0.5-2.0% solids, w/v, 50 mL) of an amylomaize starch dextrin at 90°C under different conditions	135:250	Policosanol (50-100mg) was dispersed in an aqueous solution (0.5-2.0% solids, w/v, 50 mL) of an amylomaize starch dextrin at 90°C under different conditions.
25659682	0	4	theme	composites	83:92	arg1	dispersions					44:54	aqueous dispersions	36:54	aqueous dispersions of dextrin and policosanol composites	36:92	Preparation and characterization of aqueous dispersions of dextrin and policosanol composites.
25659682	7	5	theme	policosanol	1008:1018	arg1	dispersion					1020:1029	The policosanol dispersion	1004:1029	The policosanol dispersion	1004:1029	The policosanol dispersion was sonicated up to 30s to evaluate physical stability.
25659682	6	6	theme	complex	964:970	arg1	agent					980:984	a complex forming agent	962:984	the dispersed policosanol as well as a complex forming agent with policosanol	925:1001	Among the dispersible policosanol (95%), 70% policosanol resided in the precipitates and 25% was in the supernatant, indicating the dextrin behaved as a stabilizer for the dispersed policosanol as well as a complex forming agent with policosanol.
25659682	3	7	contain	contained	439:447	arg1	precipitates					366:377	The precipitates	362:377	The precipitates obtained by centrifuging the dispersion (5,000 × g, 30 min)	362:437	The precipitates obtained by centrifuging the dispersion (5,000 × g, 30 min) contained crystalline V-amylose complex of policosanol.
25659682	3	7	contain	contained	439:447	arg2	complex					471:477	crystalline V-amylose complex	449:477	crystalline V-amylose complex of policosanol	449:492	The precipitates obtained by centrifuging the dispersion (5,000 × g, 30 min) contained crystalline V-amylose complex of policosanol.
25659682	5	8	theme	longer	604:609	arg1	time					611:614	longer time	604:614	longer time complex formation	604:632	Stepwise addition of policosanol and longer time complex formation increased the dispersible policosanol: about 95% by 30 min interval, 1.0% dextrin solution and 12h complex formation time.
25659682	4	9	contain	contained	516:524	arg1	supernatant					499:509	The supernatant	495:509	The supernatant	495:509	The supernatant also contained policosanol but not in the complex form.
25659682	4	9	contain	contained	516:524	arg2	policosanol					526:536	policosanol	526:536	policosanol	526:536	The supernatant also contained policosanol but not in the complex form.
25659682	6	10	theme	%	800:800	arg1	policosanol					802:812	70% policosanol	798:812	70% policosanol	798:812	Among the dispersible policosanol (95%), 70% policosanol resided in the precipitates and 25% was in the supernatant, indicating the dextrin behaved as a stabilizer for the dispersed policosanol as well as a complex forming agent with policosanol.
25659682	5	11	theme	time	611:614	arg1	addition					576:583	Stepwise addition	567:583	Stepwise addition of policosanol and longer time complex formation	567:632	Stepwise addition of policosanol and longer time complex formation increased the dispersible policosanol: about 95% by 30 min interval, 1.0% dextrin solution and 12h complex formation time.
25659682	3	12	theme	policosanol	482:492	arg1	complex					471:477	crystalline V-amylose complex	449:477	crystalline V-amylose complex of policosanol	449:492	The precipitates obtained by centrifuging the dispersion (5,000 × g, 30 min) contained crystalline V-amylose complex of policosanol.
25659682	7	13	theme	physical	1067:1074	arg1	stability					1076:1084	physical stability	1067:1084	physical stability	1067:1084	The policosanol dispersion was sonicated up to 30s to evaluate physical stability.
25659682	6	14	theme	70	798:799	arg1	%					800:800	%	800:800	%	800:800	Among the dispersible policosanol (95%), 70% policosanol resided in the precipitates and 25% was in the supernatant, indicating the dextrin behaved as a stabilizer for the dispersed policosanol as well as a complex forming agent with policosanol.
25659682	1	15	from	solution	146:153	arg1	90°C					220:223	90°C	220:223	90°C under different conditions	220:250	Policosanol (50-100mg) was dispersed in an aqueous solution (0.5-2.0% solids, w/v, 50 mL) of an amylomaize starch dextrin at 90°C under different conditions.
25659682	3	16	theme	V-amylose	461:469	arg1	complex					471:477	crystalline V-amylose complex	449:477	crystalline V-amylose complex of policosanol	449:492	The precipitates obtained by centrifuging the dispersion (5,000 × g, 30 min) contained crystalline V-amylose complex of policosanol.
25659682	6	17	theme	dispersed	929:937	arg1	policosanol					939:949	the dispersed policosanol	925:949	the dispersed policosanol as well as a complex forming agent with policosanol	925:1001	Among the dispersible policosanol (95%), 70% policosanol resided in the precipitates and 25% was in the supernatant, indicating the dextrin behaved as a stabilizer for the dispersed policosanol as well as a complex forming agent with policosanol.
25659682	5	18	theme	30	686:687	arg1	min					689:691	min	689:691	min	689:691	Stepwise addition of policosanol and longer time complex formation increased the dispersible policosanol: about 95% by 30 min interval, 1.0% dextrin solution and 12h complex formation time.
25659682	5	19	theme	%	706:706	arg1	solution					716:723	1.0% dextrin solution	703:723	1.0% dextrin solution	703:723	Stepwise addition of policosanol and longer time complex formation increased the dispersible policosanol: about 95% by 30 min interval, 1.0% dextrin solution and 12h complex formation time.
25659682	5	20	dep	time	611:614	arg1	formation					624:632	complex formation	616:632	longer time complex formation	604:632	Stepwise addition of policosanol and longer time complex formation increased the dispersible policosanol: about 95% by 30 min interval, 1.0% dextrin solution and 12h complex formation time.
25659682	5	21	theme	12h	729:731	arg1	formation					741:749	12h complex formation	729:749	12h complex formation	729:749	Stepwise addition of policosanol and longer time complex formation increased the dispersible policosanol: about 95% by 30 min interval, 1.0% dextrin solution and 12h complex formation time.
25659682	5	22	theme	1.0	703:705	arg1	%					706:706	%	706:706	%	706:706	Stepwise addition of policosanol and longer time complex formation increased the dispersible policosanol: about 95% by 30 min interval, 1.0% dextrin solution and 12h complex formation time.
25659682	5	23	theme	min	689:691	arg1	interval					693:700	30 min interval	686:700	30 min interval	686:700	Stepwise addition of policosanol and longer time complex formation increased the dispersible policosanol: about 95% by 30 min interval, 1.0% dextrin solution and 12h complex formation time.
25659682	2	24	theme	mixture	275:281	arg1	opaque					287:292	opaque	287:292	opaque	287:292	The dispersion of the mixture was opaque but remained homogenous for up to 7 days at an ambient temperature.
25659682	2	24	theme	mixture	275:281	arg1	dispersion					257:266	The dispersion	253:266	The dispersion of the mixture	253:281	The dispersion of the mixture was opaque but remained homogenous for up to 7 days at an ambient temperature.
25659682	8	25	from	policosanol	1098:1108	arg1	dispersion					1117:1126	the dispersion	1113:1126	the dispersion	1113:1126	Around 70% policosanol in the dispersion remained stable against the sonication treatment.
25659682	5	26	theme	complex	733:739	arg1	formation					741:749	12h complex formation	729:749	12h complex formation	729:749	Stepwise addition of policosanol and longer time complex formation increased the dispersible policosanol: about 95% by 30 min interval, 1.0% dextrin solution and 12h complex formation time.
25659682	1	27	theme	different	231:239	arg1	conditions					241:250	different conditions	231:250	different conditions	231:250	Policosanol (50-100mg) was dispersed in an aqueous solution (0.5-2.0% solids, w/v, 50 mL) of an amylomaize starch dextrin at 90°C under different conditions.
25659682	3	28	dep	dispersion	408:417	arg1	g					428:428	5,000 × g	420:428	5,000 × g	420:428	The precipitates obtained by centrifuging the dispersion (5,000 × g, 30 min) contained crystalline V-amylose complex of policosanol.
25659682	3	28	dep	dispersion	408:417	arg1	min					434:436	30 min	431:436	30 min	431:436	The precipitates obtained by centrifuging the dispersion (5,000 × g, 30 min) contained crystalline V-amylose complex of policosanol.
25659682	5	29	dep	policosanol	660:670	arg1	%					681:681	about 95%	673:681	the dispersible policosanol: about 95%	644:681	Stepwise addition of policosanol and longer time complex formation increased the dispersible policosanol: about 95% by 30 min interval, 1.0% dextrin solution and 12h complex formation time.
25659682	8	30	theme	Around	1087:1092	arg1	policosanol					1098:1108	70% policosanol	1094:1108	Around 70% policosanol in the dispersion	1087:1126	Around 70% policosanol in the dispersion remained stable against the sonication treatment.
25659682	1	31	from	90°C	220:223	arg1	solution					146:153	an aqueous solution	135:153	an aqueous solution (0.5-2.0% solids, w/v, 50 mL) of an amylomaize starch dextrin at 90°C under different conditions	135:250	Policosanol (50-100mg) was dispersed in an aqueous solution (0.5-2.0% solids, w/v, 50 mL) of an amylomaize starch dextrin at 90°C under different conditions.
25659682	1	31	from	90°C	220:223	arg1	dextrin					209:215	an amylomaize starch dextrin	188:215	an amylomaize starch dextrin at 90°C under different conditions	188:250	Policosanol (50-100mg) was dispersed in an aqueous solution (0.5-2.0% solids, w/v, 50 mL) of an amylomaize starch dextrin at 90°C under different conditions.
25659682	8	32	theme	%	1096:1096	arg1	policosanol					1098:1108	70% policosanol	1094:1108	Around 70% policosanol in the dispersion	1087:1126	Around 70% policosanol in the dispersion remained stable against the sonication treatment.
25659682	1	33	dep	solution	146:153	arg1	%					163:163	0.5-2.0% solids	156:170	0.5-2.0% solids	156:170	Policosanol (50-100mg) was dispersed in an aqueous solution (0.5-2.0% solids, w/v, 50 mL) of an amylomaize starch dextrin at 90°C under different conditions.
25659682	1	33	dep	solution	146:153	arg1	w/v					173:175	w/v	173:175	w/v	173:175	Policosanol (50-100mg) was dispersed in an aqueous solution (0.5-2.0% solids, w/v, 50 mL) of an amylomaize starch dextrin at 90°C under different conditions.
25659682	1	33	dep	solution	146:153	arg1	mL					181:182	50 mL	178:182	50 mL	178:182	Policosanol (50-100mg) was dispersed in an aqueous solution (0.5-2.0% solids, w/v, 50 mL) of an amylomaize starch dextrin at 90°C under different conditions.
25659682	3	34	theme	5,000	420:424	arg1	×					426:426	×	426:426	×	426:426	The precipitates obtained by centrifuging the dispersion (5,000 × g, 30 min) contained crystalline V-amylose complex of policosanol.
25659682	0	35	theme	dispersions	44:54	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of aqueous dispersions of dextrin and policosanol composites.
25659682	0	35	theme	dispersions	44:54	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of aqueous dispersions of dextrin and policosanol composites.
25659682	0	36	theme	aqueous	36:42	arg1	dispersions					44:54	aqueous dispersions	36:54	aqueous dispersions of dextrin and policosanol composites	36:92	Preparation and characterization of aqueous dispersions of dextrin and policosanol composites.
25659682	5	37	theme	dextrin	708:714	arg1	solution					716:723	1.0% dextrin solution	703:723	1.0% dextrin solution	703:723	Stepwise addition of policosanol and longer time complex formation increased the dispersible policosanol: about 95% by 30 min interval, 1.0% dextrin solution and 12h complex formation time.
25659682	2	38	theme	ambient	341:347	arg1	temperature					349:359	an ambient temperature	338:359	an ambient temperature	338:359	The dispersion of the mixture was opaque but remained homogenous for up to 7 days at an ambient temperature.
25659682	0	39	theme	dextrin	59:65	arg1	composites					83:92	dextrin and policosanol composites	59:92	dextrin and policosanol composites	59:92	Preparation and characterization of aqueous dispersions of dextrin and policosanol composites.
25659682	5	40	theme	complex	616:622	arg1	formation					624:632	complex formation	616:632	longer time complex formation	604:632	Stepwise addition of policosanol and longer time complex formation increased the dispersible policosanol: about 95% by 30 min interval, 1.0% dextrin solution and 12h complex formation time.
25659682	1	41	dep	%	163:163	arg1	solids					165:170	solids	165:170	0.5-2.0% solids	156:170	Policosanol (50-100mg) was dispersed in an aqueous solution (0.5-2.0% solids, w/v, 50 mL) of an amylomaize starch dextrin at 90°C under different conditions.
25659682	5	42	dep	interval	693:700	arg1	time					751:754	time	751:754	time	751:754	Stepwise addition of policosanol and longer time complex formation increased the dispersible policosanol: about 95% by 30 min interval, 1.0% dextrin solution and 12h complex formation time.
25659682	5	43	theme	dispersible	648:658	arg1	policosanol					660:670	the dispersible policosanol	644:670	the dispersible policosanol: about 95%	644:681	Stepwise addition of policosanol and longer time complex formation increased the dispersible policosanol: about 95% by 30 min interval, 1.0% dextrin solution and 12h complex formation time.
25659682	6	44	theme	dispersible	767:777	arg1	policosanol					779:789	the dispersible policosanol	763:789	the dispersible policosanol (95%)	763:795	Among the dispersible policosanol (95%), 70% policosanol resided in the precipitates and 25% was in the supernatant, indicating the dextrin behaved as a stabilizer for the dispersed policosanol as well as a complex forming agent with policosanol.
25659682	6	44	theme	dispersible	767:777	arg1	%					794:794	95%	792:794	95%	792:794	Among the dispersible policosanol (95%), 70% policosanol resided in the precipitates and 25% was in the supernatant, indicating the dextrin behaved as a stabilizer for the dispersed policosanol as well as a complex forming agent with policosanol.
25659682	5	45	theme	Stepwise	567:574	arg1	addition					576:583	Stepwise addition	567:583	Stepwise addition of policosanol and longer time complex formation	567:632	Stepwise addition of policosanol and longer time complex formation increased the dispersible policosanol: about 95% by 30 min interval, 1.0% dextrin solution and 12h complex formation time.
25659682	3	46	theme	×	426:426	arg1	g					428:428	5,000 × g	420:428	5,000 × g	420:428	The precipitates obtained by centrifuging the dispersion (5,000 × g, 30 min) contained crystalline V-amylose complex of policosanol.
25659682	3	46	theme	×	426:426	arg1	min					434:436	30 min	431:436	30 min	431:436	The precipitates obtained by centrifuging the dispersion (5,000 × g, 30 min) contained crystalline V-amylose complex of policosanol.
25659682	6	47	with	policosanol	939:949	arg1	policosanol					991:1001	policosanol	991:1001	policosanol	991:1001	Among the dispersible policosanol (95%), 70% policosanol resided in the precipitates and 25% was in the supernatant, indicating the dextrin behaved as a stabilizer for the dispersed policosanol as well as a complex forming agent with policosanol.
25659682	2	48	dep	7	328:328	arg1	to					325:326	to	325:326	to	325:326	The dispersion of the mixture was opaque but remained homogenous for up to 7 days at an ambient temperature.
25659682	8	49	theme	70	1094:1095	arg1	%					1096:1096	%	1096:1096	%	1096:1096	Around 70% policosanol in the dispersion remained stable against the sonication treatment.
25659682	0	50	theme	policosanol	71:81	arg1	composites					83:92	dextrin and policosanol composites	59:92	dextrin and policosanol composites	59:92	Preparation and characterization of aqueous dispersions of dextrin and policosanol composites.
25659682	8	51	theme	sonication	1156:1165	arg1	treatment					1167:1175	the sonication treatment	1152:1175	the sonication treatment	1152:1175	Around 70% policosanol in the dispersion remained stable against the sonication treatment.
25659682	1	52	theme	aqueous	138:144	arg1	solution					146:153	an aqueous solution	135:153	an aqueous solution (0.5-2.0% solids, w/v, 50 mL) of an amylomaize starch dextrin at 90°C under different conditions	135:250	Policosanol (50-100mg) was dispersed in an aqueous solution (0.5-2.0% solids, w/v, 50 mL) of an amylomaize starch dextrin at 90°C under different conditions.
25659682	1	53	theme	amylomaize	191:200	arg1	dextrin					209:215	an amylomaize starch dextrin	188:215	an amylomaize starch dextrin at 90°C under different conditions	188:250	Policosanol (50-100mg) was dispersed in an aqueous solution (0.5-2.0% solids, w/v, 50 mL) of an amylomaize starch dextrin at 90°C under different conditions.
25659682	4	54	theme	complex	553:559	arg1	form					561:564	the complex form	549:564	the complex form	549:564	The supernatant also contained policosanol but not in the complex form.
25659682	3	55	theme	crystalline	449:459	arg1	complex					471:477	crystalline V-amylose complex	449:477	crystalline V-amylose complex of policosanol	449:492	The precipitates obtained by centrifuging the dispersion (5,000 × g, 30 min) contained crystalline V-amylose complex of policosanol.
25659682	1	56	theme	starch	202:207	arg1	dextrin					209:215	an amylomaize starch dextrin	188:215	an amylomaize starch dextrin at 90°C under different conditions	188:250	Policosanol (50-100mg) was dispersed in an aqueous solution (0.5-2.0% solids, w/v, 50 mL) of an amylomaize starch dextrin at 90°C under different conditions.
26311190	2	0	theme	large	509:513	arg1	granules					529:536	large A-type starch granules	509:536	large A-type starch granules at 12 d after anthesis (DAA) and a high percentage of B-type starch granules at 18 DAA in endosperm cells of the two wheat cultivars	509:669	RESULTS DS resulted in large A-type starch granules at 12 d after anthesis (DAA) and a high percentage of B-type starch granules at 18 DAA in endosperm cells of the two wheat cultivars.
26311190	8	1	theme	final	1483:1487	arg1	products					1495:1502	the final wheat products	1479:1502	the final wheat products	1479:1502	CONCLUSION Overall, DS can affect the development of endosperm starch granules and the physicochemical properties of starches, thus affecting the qualities of the final wheat products.
26311190	7	2	theme	gelatinised	1281:1291	arg1	starches					1310:1317	native, gelatinised, and retrograded starches	1273:1317	native, gelatinised, and retrograded starches	1273:1317	DS altered the contents of rapidly digestible, slowly digestible, and resistant starches in native, gelatinised, and retrograded starches.
26311190	8	3	theme	products	1495:1502	arg1	qualities					1466:1474	the qualities	1462:1474	the qualities of the final wheat products	1462:1502	CONCLUSION Overall, DS can affect the development of endosperm starch granules and the physicochemical properties of starches, thus affecting the qualities of the final wheat products.
26311190	6	4	theme	Aspergillus	1128:1138	arg1	niger					1140:1144	Aspergillus niger	1128:1144	Aspergillus niger amyloglucosidase	1128:1161	DS also significantly enhanced the hydrolysis degrees of starches by pancreatic α-amylase, Aspergillus niger amyloglucosidase, and HCl in NM13.
26311190	1	5	theme	physicochemical	371:385	arg1	properties					387:396	physicochemical properties	371:396	physicochemical properties	371:396	BACKGROUND The objectives of this study were to: (1) observe the effects of drought stress (DS) on the structural development of endosperm starch granules; (2) investigate the effects of DS on composition and physicochemical properties of starches; and (3) compare the different responses to DS between soft and hard wheat.
26311190	7	6	theme	native	1273:1278	arg1	starches					1310:1317	native, gelatinised, and retrograded starches	1273:1317	native, gelatinised, and retrograded starches	1273:1317	DS altered the contents of rapidly digestible, slowly digestible, and resistant starches in native, gelatinised, and retrograded starches.
26311190	7	7	theme	retrograded	1298:1308	arg1	starches					1310:1317	native, gelatinised, and retrograded starches	1273:1317	native, gelatinised, and retrograded starches	1273:1317	DS altered the contents of rapidly digestible, slowly digestible, and resistant starches in native, gelatinised, and retrograded starches.
26311190	2	8	theme	starch	522:527	arg1	granules					529:536	large A-type starch granules	509:536	large A-type starch granules at 12 d after anthesis (DAA) and a high percentage of B-type starch granules at 18 DAA in endosperm cells of the two wheat cultivars	509:669	RESULTS DS resulted in large A-type starch granules at 12 d after anthesis (DAA) and a high percentage of B-type starch granules at 18 DAA in endosperm cells of the two wheat cultivars.
26311190	7	9	theme	starches	1261:1268	arg1	contents					1196:1203	the contents	1192:1203	the contents of rapidly digestible, slowly digestible, and resistant starches in native, gelatinised, and retrograded starches	1192:1317	DS altered the contents of rapidly digestible, slowly digestible, and resistant starches in native, gelatinised, and retrograded starches.
26311190	1	10	from	effects	227:233	arg1	development					276:286	the structural development	261:286	the structural development of endosperm starch granules	261:315	BACKGROUND The objectives of this study were to: (1) observe the effects of drought stress (DS) on the structural development of endosperm starch granules; (2) investigate the effects of DS on composition and physicochemical properties of starches; and (3) compare the different responses to DS between soft and hard wheat.
26311190	7	11	theme	digestible	1235:1244	arg1	contents					1196:1203	the contents	1192:1203	the contents of rapidly digestible, slowly digestible, and resistant starches in native, gelatinised, and retrograded starches	1192:1317	DS altered the contents of rapidly digestible, slowly digestible, and resistant starches in native, gelatinised, and retrograded starches.
26311190	0	12	theme	starches	127:134	arg1	composition					81:91	composition	81:91	composition	81:91	Effect of drought stress on the development of endosperm starch granules and the composition and physicochemical properties of starches from soft and hard wheat.
26311190	0	12	theme	starches	127:134	arg1	properties					113:122	physicochemical properties	97:122	physicochemical properties	97:122	Effect of drought stress on the development of endosperm starch granules and the composition and physicochemical properties of starches from soft and hard wheat.
26311190	0	12	theme	starches	127:134	arg1	development					32:42	the development	28:42	the development of endosperm starch granules	28:71	Effect of drought stress on the development of endosperm starch granules and the composition and physicochemical properties of starches from soft and hard wheat.
26311190	2	13	theme	RESULTS	486:492	arg1	DS					494:495	RESULTS DS	486:495	RESULTS DS	486:495	RESULTS DS resulted in large A-type starch granules at 12 d after anthesis (DAA) and a high percentage of B-type starch granules at 18 DAA in endosperm cells of the two wheat cultivars.
26311190	1	14	theme	drought	238:244	arg1	stress					246:251	drought stress	238:251	drought stress (DS)	238:256	BACKGROUND The objectives of this study were to: (1) observe the effects of drought stress (DS) on the structural development of endosperm starch granules; (2) investigate the effects of DS on composition and physicochemical properties of starches; and (3) compare the different responses to DS between soft and hard wheat.
26311190	1	14	theme	drought	238:244	arg1	DS					254:255	DS	254:255	DS	254:255	BACKGROUND The objectives of this study were to: (1) observe the effects of drought stress (DS) on the structural development of endosperm starch granules; (2) investigate the effects of DS on composition and physicochemical properties of starches; and (3) compare the different responses to DS between soft and hard wheat.
26311190	2	15	from	d	544:544	arg1	granules					529:536	large A-type starch granules	509:536	large A-type starch granules at 12 d after anthesis (DAA) and a high percentage of B-type starch granules at 18 DAA in endosperm cells of the two wheat cultivars	509:669	RESULTS DS resulted in large A-type starch granules at 12 d after anthesis (DAA) and a high percentage of B-type starch granules at 18 DAA in endosperm cells of the two wheat cultivars.
26311190	3	16	theme	starch	714:719	arg1	contents					733:740	total starch and amylose contents	708:740	total starch and amylose contents	708:740	DS decreased the 1000-grain weight, total starch and amylose contents, and amylose-to-amylopectin ratio of both starches.
26311190	2	17	from	anthesis	552:559	arg1	DAA					621:623	18 DAA	618:623	18 DAA in endosperm cells of the two wheat cultivars	618:669	RESULTS DS resulted in large A-type starch granules at 12 d after anthesis (DAA) and a high percentage of B-type starch granules at 18 DAA in endosperm cells of the two wheat cultivars.
26311190	1	18	theme	DS	349:350	arg1	effects					338:344	the effects	334:344	the effects of DS on composition and physicochemical properties of starches	334:408	BACKGROUND The objectives of this study were to: (1) observe the effects of drought stress (DS) on the structural development of endosperm starch granules; (2) investigate the effects of DS on composition and physicochemical properties of starches; and (3) compare the different responses to DS between soft and hard wheat.
26311190	0	19	dep	soft	141:144	arg1	wheat					155:159	wheat	155:159	wheat	155:159	Effect of drought stress on the development of endosperm starch granules and the composition and physicochemical properties of starches from soft and hard wheat.
26311190	4	20	theme	starch	837:842	arg1	granules					844:851	B-type starch granules	830:851	B-type starch granules	830:851	DS also decreased the percentage of B-type starch granules in NM13 and increased the number of hollows on the surface of A-type starch granules in XM33.
26311190	2	21	theme	granules	606:613	arg1	percentage					578:587	a high percentage	571:587	a high percentage of B-type starch granules at 18 DAA in endosperm cells of the two wheat cultivars	571:669	RESULTS DS resulted in large A-type starch granules at 12 d after anthesis (DAA) and a high percentage of B-type starch granules at 18 DAA in endosperm cells of the two wheat cultivars.
26311190	2	21	theme	granules	606:613	arg1	DAA					562:564	DAA	562:564	DAA	562:564	RESULTS DS resulted in large A-type starch granules at 12 d after anthesis (DAA) and a high percentage of B-type starch granules at 18 DAA in endosperm cells of the two wheat cultivars.
26311190	2	21	theme	granules	606:613	arg1	anthesis					552:559	anthesis	552:559	anthesis (DAA)	552:565	RESULTS DS resulted in large A-type starch granules at 12 d after anthesis (DAA) and a high percentage of B-type starch granules at 18 DAA in endosperm cells of the two wheat cultivars.
26311190	0	22	theme	endosperm	47:55	arg1	granules					64:71	endosperm starch granules	47:71	endosperm starch granules	47:71	Effect of drought stress on the development of endosperm starch granules and the composition and physicochemical properties of starches from soft and hard wheat.
26311190	1	23	theme	structural	265:274	arg1	development					276:286	the structural development	261:286	the structural development of endosperm starch granules	261:315	BACKGROUND The objectives of this study were to: (1) observe the effects of drought stress (DS) on the structural development of endosperm starch granules; (2) investigate the effects of DS on composition and physicochemical properties of starches; and (3) compare the different responses to DS between soft and hard wheat.
26311190	2	24	theme	B-type	592:597	arg1	granules					606:613	B-type starch granules	592:613	B-type starch granules at 18 DAA in endosperm cells of the two wheat cultivars	592:669	RESULTS DS resulted in large A-type starch granules at 12 d after anthesis (DAA) and a high percentage of B-type starch granules at 18 DAA in endosperm cells of the two wheat cultivars.
26311190	3	25	theme	1000-grain	689:698	arg1	weight					700:705	1000-grain weight	689:705	1000-grain weight	689:705	DS decreased the 1000-grain weight, total starch and amylose contents, and amylose-to-amylopectin ratio of both starches.
26311190	0	26	theme	granules	64:71	arg1	composition					81:91	composition	81:91	composition	81:91	Effect of drought stress on the development of endosperm starch granules and the composition and physicochemical properties of starches from soft and hard wheat.
26311190	0	26	theme	granules	64:71	arg1	properties					113:122	physicochemical properties	97:122	physicochemical properties	97:122	Effect of drought stress on the development of endosperm starch granules and the composition and physicochemical properties of starches from soft and hard wheat.
26311190	0	26	theme	granules	64:71	arg1	development					32:42	the development	28:42	the development of endosperm starch granules	28:71	Effect of drought stress on the development of endosperm starch granules and the composition and physicochemical properties of starches from soft and hard wheat.
26311190	3	27	theme	amylose	725:731	arg1	contents					733:740	total starch and amylose contents	708:740	total starch and amylose contents	708:740	DS decreased the 1000-grain weight, total starch and amylose contents, and amylose-to-amylopectin ratio of both starches.
26311190	0	28	from	Effect	0:5	arg1	composition					81:91	composition	81:91	composition	81:91	Effect of drought stress on the development of endosperm starch granules and the composition and physicochemical properties of starches from soft and hard wheat.
26311190	0	28	from	Effect	0:5	arg1	development					32:42	the development	28:42	the development of endosperm starch granules	28:71	Effect of drought stress on the development of endosperm starch granules and the composition and physicochemical properties of starches from soft and hard wheat.
26311190	1	29	theme	starch	301:306	arg1	granules					308:315	endosperm starch granules	291:315	endosperm starch granules	291:315	BACKGROUND The objectives of this study were to: (1) observe the effects of drought stress (DS) on the structural development of endosperm starch granules; (2) investigate the effects of DS on composition and physicochemical properties of starches; and (3) compare the different responses to DS between soft and hard wheat.
26311190	7	30	from	contents	1196:1203	arg1	starches					1310:1317	native, gelatinised, and retrograded starches	1273:1317	native, gelatinised, and retrograded starches	1273:1317	DS altered the contents of rapidly digestible, slowly digestible, and resistant starches in native, gelatinised, and retrograded starches.
26311190	0	31	from	development	32:42	arg1	hard					150:153	hard	150:153	hard	150:153	Effect of drought stress on the development of endosperm starch granules and the composition and physicochemical properties of starches from soft and hard wheat.
26311190	0	31	from	development	32:42	arg1	soft					141:144	soft	141:144	soft	141:144	Effect of drought stress on the development of endosperm starch granules and the composition and physicochemical properties of starches from soft and hard wheat.
26311190	8	32	theme	wheat	1489:1493	arg1	products					1495:1502	the final wheat products	1479:1502	the final wheat products	1479:1502	CONCLUSION Overall, DS can affect the development of endosperm starch granules and the physicochemical properties of starches, thus affecting the qualities of the final wheat products.
26311190	3	33	theme	amylose-to-amylopectin	747:768	arg1	ratio					770:774	the 1000-grain weight, total starch and amylose contents, and amylose-to-amylopectin ratio	685:774	the 1000-grain weight, total starch and amylose contents, and amylose-to-amylopectin ratio of both starches	685:791	DS decreased the 1000-grain weight, total starch and amylose contents, and amylose-to-amylopectin ratio of both starches.
26311190	8	34	theme	starch	1383:1388	arg1	granules					1390:1397	endosperm starch granules	1373:1397	endosperm starch granules	1373:1397	CONCLUSION Overall, DS can affect the development of endosperm starch granules and the physicochemical properties of starches, thus affecting the qualities of the final wheat products.
26311190	0	35	theme	drought	10:16	arg1	stress					18:23	drought stress	10:23	drought stress	10:23	Effect of drought stress on the development of endosperm starch granules and the composition and physicochemical properties of starches from soft and hard wheat.
26311190	1	36	theme	stress	246:251	arg1	effects					227:233	the effects	223:233	the effects of drought stress (DS) on the structural development of endosperm starch granules	223:315	BACKGROUND The objectives of this study were to: (1) observe the effects of drought stress (DS) on the structural development of endosperm starch granules; (2) investigate the effects of DS on composition and physicochemical properties of starches; and (3) compare the different responses to DS between soft and hard wheat.
26311190	3	37	theme	starches	784:791	arg1	ratio					770:774	the 1000-grain weight, total starch and amylose contents, and amylose-to-amylopectin ratio	685:774	the 1000-grain weight, total starch and amylose contents, and amylose-to-amylopectin ratio of both starches	685:791	DS decreased the 1000-grain weight, total starch and amylose contents, and amylose-to-amylopectin ratio of both starches.
26311190	8	38	theme	physicochemical	1407:1421	arg1	properties					1423:1432	the physicochemical properties	1403:1432	the physicochemical properties of starches	1403:1444	CONCLUSION Overall, DS can affect the development of endosperm starch granules and the physicochemical properties of starches, thus affecting the qualities of the final wheat products.
26311190	2	39	from	DAA	621:623	arg1	percentage					578:587	a high percentage	571:587	a high percentage of B-type starch granules at 18 DAA in endosperm cells of the two wheat cultivars	571:669	RESULTS DS resulted in large A-type starch granules at 12 d after anthesis (DAA) and a high percentage of B-type starch granules at 18 DAA in endosperm cells of the two wheat cultivars.
26311190	2	39	from	DAA	621:623	arg1	DAA					562:564	DAA	562:564	DAA	562:564	RESULTS DS resulted in large A-type starch granules at 12 d after anthesis (DAA) and a high percentage of B-type starch granules at 18 DAA in endosperm cells of the two wheat cultivars.
26311190	2	39	from	DAA	621:623	arg1	anthesis					552:559	anthesis	552:559	anthesis (DAA)	552:565	RESULTS DS resulted in large A-type starch granules at 12 d after anthesis (DAA) and a high percentage of B-type starch granules at 18 DAA in endosperm cells of the two wheat cultivars.
26311190	2	39	from	DAA	621:623	arg1	granules					606:613	B-type starch granules	592:613	B-type starch granules at 18 DAA in endosperm cells of the two wheat cultivars	592:669	RESULTS DS resulted in large A-type starch granules at 12 d after anthesis (DAA) and a high percentage of B-type starch granules at 18 DAA in endosperm cells of the two wheat cultivars.
26311190	2	39	from	DAA	621:623	arg1	cells					638:642	endosperm cells	628:642	endosperm cells	628:642	RESULTS DS resulted in large A-type starch granules at 12 d after anthesis (DAA) and a high percentage of B-type starch granules at 18 DAA in endosperm cells of the two wheat cultivars.
26311190	4	40	theme	granules	929:936	arg1	surface					904:910	the surface	900:910	the surface of A-type starch granules in XM33	900:944	DS also decreased the percentage of B-type starch granules in NM13 and increased the number of hollows on the surface of A-type starch granules in XM33.
26311190	2	41	theme	cultivars	661:669	arg1	DAA					621:623	18 DAA	618:623	18 DAA in endosperm cells of the two wheat cultivars	618:669	RESULTS DS resulted in large A-type starch granules at 12 d after anthesis (DAA) and a high percentage of B-type starch granules at 18 DAA in endosperm cells of the two wheat cultivars.
26311190	1	42	theme	different	431:439	arg1	responses					441:449	the different responses	427:449	the different responses to DS between soft and hard wheat	427:483	BACKGROUND The objectives of this study were to: (1) observe the effects of drought stress (DS) on the structural development of endosperm starch granules; (2) investigate the effects of DS on composition and physicochemical properties of starches; and (3) compare the different responses to DS between soft and hard wheat.
26311190	0	43	from	properties	113:122	arg1	hard					150:153	hard	150:153	hard	150:153	Effect of drought stress on the development of endosperm starch granules and the composition and physicochemical properties of starches from soft and hard wheat.
26311190	0	43	from	properties	113:122	arg1	soft					141:144	soft	141:144	soft	141:144	Effect of drought stress on the development of endosperm starch granules and the composition and physicochemical properties of starches from soft and hard wheat.
26311190	4	44	theme	A-type	915:920	arg1	granules					929:936	A-type starch granules	915:936	A-type starch granules in XM33	915:944	DS also decreased the percentage of B-type starch granules in NM13 and increased the number of hollows on the surface of A-type starch granules in XM33.
26311190	0	45	from	hard	150:153	arg1	starches					127:134	starches	127:134	starches from soft and hard wheat	127:159	Effect of drought stress on the development of endosperm starch granules and the composition and physicochemical properties of starches from soft and hard wheat.
26311190	0	45	from	hard	150:153	arg1	composition					81:91	composition	81:91	composition	81:91	Effect of drought stress on the development of endosperm starch granules and the composition and physicochemical properties of starches from soft and hard wheat.
26311190	0	45	from	hard	150:153	arg1	properties					113:122	physicochemical properties	97:122	physicochemical properties	97:122	Effect of drought stress on the development of endosperm starch granules and the composition and physicochemical properties of starches from soft and hard wheat.
26311190	0	45	from	hard	150:153	arg1	development					32:42	the development	28:42	the development of endosperm starch granules	28:71	Effect of drought stress on the development of endosperm starch granules and the composition and physicochemical properties of starches from soft and hard wheat.
26311190	6	46	theme	niger	1140:1144	arg1	amyloglucosidase					1146:1161	Aspergillus niger amyloglucosidase	1128:1161	Aspergillus niger amyloglucosidase	1128:1161	DS also significantly enhanced the hydrolysis degrees of starches by pancreatic α-amylase, Aspergillus niger amyloglucosidase, and HCl in NM13.
26311190	2	47	theme	endosperm	628:636	arg1	cells					638:642	endosperm cells	628:642	endosperm cells	628:642	RESULTS DS resulted in large A-type starch granules at 12 d after anthesis (DAA) and a high percentage of B-type starch granules at 18 DAA in endosperm cells of the two wheat cultivars.
26311190	0	48	from	soft	141:144	arg1	starches					127:134	starches	127:134	starches from soft and hard wheat	127:159	Effect of drought stress on the development of endosperm starch granules and the composition and physicochemical properties of starches from soft and hard wheat.
26311190	0	48	from	soft	141:144	arg1	composition					81:91	composition	81:91	composition	81:91	Effect of drought stress on the development of endosperm starch granules and the composition and physicochemical properties of starches from soft and hard wheat.
26311190	0	48	from	soft	141:144	arg1	properties					113:122	physicochemical properties	97:122	physicochemical properties	97:122	Effect of drought stress on the development of endosperm starch granules and the composition and physicochemical properties of starches from soft and hard wheat.
26311190	0	48	from	soft	141:144	arg1	development					32:42	the development	28:42	the development of endosperm starch granules	28:71	Effect of drought stress on the development of endosperm starch granules and the composition and physicochemical properties of starches from soft and hard wheat.
26311190	9	49	theme	Chemical	1523:1530	arg1	Industry					1532:1539	Chemical Industry	1523:1539	Chemical Industry	1523:1539	© 2015 Society of Chemical Industry.
26311190	6	50	theme	pancreatic	1106:1115	arg1	α-amylase					1117:1125	pancreatic α-amylase	1106:1125	pancreatic α-amylase	1106:1125	DS also significantly enhanced the hydrolysis degrees of starches by pancreatic α-amylase, Aspergillus niger amyloglucosidase, and HCl in NM13.
26311190	1	51	theme	starches	401:408	arg1	composition					355:365	composition	355:365	composition	355:365	BACKGROUND The objectives of this study were to: (1) observe the effects of drought stress (DS) on the structural development of endosperm starch granules; (2) investigate the effects of DS on composition and physicochemical properties of starches; and (3) compare the different responses to DS between soft and hard wheat.
26311190	1	51	theme	starches	401:408	arg1	properties					387:396	physicochemical properties	371:396	physicochemical properties	371:396	BACKGROUND The objectives of this study were to: (1) observe the effects of drought stress (DS) on the structural development of endosperm starch granules; (2) investigate the effects of DS on composition and physicochemical properties of starches; and (3) compare the different responses to DS between soft and hard wheat.
26311190	2	52	theme	A-type	515:520	arg1	granules					529:536	large A-type starch granules	509:536	large A-type starch granules at 12 d after anthesis (DAA) and a high percentage of B-type starch granules at 18 DAA in endosperm cells of the two wheat cultivars	509:669	RESULTS DS resulted in large A-type starch granules at 12 d after anthesis (DAA) and a high percentage of B-type starch granules at 18 DAA in endosperm cells of the two wheat cultivars.
26311190	2	53	from	percentage	578:587	arg1	DAA					621:623	18 DAA	618:623	18 DAA in endosperm cells of the two wheat cultivars	618:669	RESULTS DS resulted in large A-type starch granules at 12 d after anthesis (DAA) and a high percentage of B-type starch granules at 18 DAA in endosperm cells of the two wheat cultivars.
26311190	7	54	theme	resistant	1251:1259	arg1	starches					1261:1268	resistant starches	1251:1268	resistant starches	1251:1268	DS altered the contents of rapidly digestible, slowly digestible, and resistant starches in native, gelatinised, and retrograded starches.
26311190	1	55	theme	study	196:200	arg1	objectives					177:186	The objectives	173:186	BACKGROUND The objectives of this study	162:200	BACKGROUND The objectives of this study were to: (1) observe the effects of drought stress (DS) on the structural development of endosperm starch granules; (2) investigate the effects of DS on composition and physicochemical properties of starches; and (3) compare the different responses to DS between soft and hard wheat.
26311190	1	56	dep	to	207:208	arg1	investigate					322:332	investigate	322:332	investigate the effects of DS on composition and physicochemical properties of starches	322:408	BACKGROUND The objectives of this study were to: (1) observe the effects of drought stress (DS) on the structural development of endosperm starch granules; (2) investigate the effects of DS on composition and physicochemical properties of starches; and (3) compare the different responses to DS between soft and hard wheat.
26311190	1	56	dep	to	207:208	arg1	observe					215:221	observe	215:221	observe the effects of drought stress (DS) on the structural development of endosperm starch granules	215:315	BACKGROUND The objectives of this study were to: (1) observe the effects of drought stress (DS) on the structural development of endosperm starch granules; (2) investigate the effects of DS on composition and physicochemical properties of starches; and (3) compare the different responses to DS between soft and hard wheat.
26311190	1	56	dep	to	207:208	arg1	2					319:319	2	319:319	2	319:319	BACKGROUND The objectives of this study were to: (1) observe the effects of drought stress (DS) on the structural development of endosperm starch granules; (2) investigate the effects of DS on composition and physicochemical properties of starches; and (3) compare the different responses to DS between soft and hard wheat.
26311190	1	56	dep	to	207:208	arg1	compare					419:425	compare	419:425	compare the different responses to DS between soft and hard wheat	419:483	BACKGROUND The objectives of this study were to: (1) observe the effects of drought stress (DS) on the structural development of endosperm starch granules; (2) investigate the effects of DS on composition and physicochemical properties of starches; and (3) compare the different responses to DS between soft and hard wheat.
26311190	1	56	dep	to	207:208	arg1	1					212:212	1	212:212	1	212:212	BACKGROUND The objectives of this study were to: (1) observe the effects of drought stress (DS) on the structural development of endosperm starch granules; (2) investigate the effects of DS on composition and physicochemical properties of starches; and (3) compare the different responses to DS between soft and hard wheat.
26311190	4	57	from	XM33	941:944	arg1	surface					904:910	the surface	900:910	the surface of A-type starch granules in XM33	900:944	DS also decreased the percentage of B-type starch granules in NM13 and increased the number of hollows on the surface of A-type starch granules in XM33.
26311190	6	58	theme	hydrolysis	1072:1081	arg1	degrees					1083:1089	the hydrolysis degrees	1068:1089	the hydrolysis degrees of starches	1068:1101	DS also significantly enhanced the hydrolysis degrees of starches by pancreatic α-amylase, Aspergillus niger amyloglucosidase, and HCl in NM13.
26311190	2	59	theme	high	573:576	arg1	percentage					578:587	a high percentage	571:587	a high percentage of B-type starch granules at 18 DAA in endosperm cells of the two wheat cultivars	571:669	RESULTS DS resulted in large A-type starch granules at 12 d after anthesis (DAA) and a high percentage of B-type starch granules at 18 DAA in endosperm cells of the two wheat cultivars.
26311190	4	60	from	granules	929:936	arg1	XM33					941:944	XM33	941:944	XM33	941:944	DS also decreased the percentage of B-type starch granules in NM13 and increased the number of hollows on the surface of A-type starch granules in XM33.
26311190	0	61	from	composition	81:91	arg1	hard					150:153	hard	150:153	hard	150:153	Effect of drought stress on the development of endosperm starch granules and the composition and physicochemical properties of starches from soft and hard wheat.
26311190	0	61	from	composition	81:91	arg1	soft					141:144	soft	141:144	soft	141:144	Effect of drought stress on the development of endosperm starch granules and the composition and physicochemical properties of starches from soft and hard wheat.
26311190	7	62	theme	digestible	1216:1225	arg1	contents					1196:1203	the contents	1192:1203	the contents of rapidly digestible, slowly digestible, and resistant starches in native, gelatinised, and retrograded starches	1192:1317	DS altered the contents of rapidly digestible, slowly digestible, and resistant starches in native, gelatinised, and retrograded starches.
26311190	0	63	theme	starch	57:62	arg1	granules					64:71	endosperm starch granules	47:71	endosperm starch granules	47:71	Effect of drought stress on the development of endosperm starch granules and the composition and physicochemical properties of starches from soft and hard wheat.
26311190	3	64	theme	total	708:712	arg1	contents					733:740	total starch and amylose contents	708:740	total starch and amylose contents	708:740	DS decreased the 1000-grain weight, total starch and amylose contents, and amylose-to-amylopectin ratio of both starches.
26311190	4	65	theme	B-type	830:835	arg1	granules					844:851	B-type starch granules	830:851	B-type starch granules	830:851	DS also decreased the percentage of B-type starch granules in NM13 and increased the number of hollows on the surface of A-type starch granules in XM33.
26311190	2	66	theme	starch	599:604	arg1	granules					606:613	B-type starch granules	592:613	B-type starch granules at 18 DAA in endosperm cells of the two wheat cultivars	592:669	RESULTS DS resulted in large A-type starch granules at 12 d after anthesis (DAA) and a high percentage of B-type starch granules at 18 DAA in endosperm cells of the two wheat cultivars.
26311190	1	67	from	effects	338:344	arg1	composition					355:365	composition	355:365	composition	355:365	BACKGROUND The objectives of this study were to: (1) observe the effects of drought stress (DS) on the structural development of endosperm starch granules; (2) investigate the effects of DS on composition and physicochemical properties of starches; and (3) compare the different responses to DS between soft and hard wheat.
26311190	1	67	from	effects	338:344	arg1	properties					387:396	physicochemical properties	371:396	physicochemical properties	371:396	BACKGROUND The objectives of this study were to: (1) observe the effects of drought stress (DS) on the structural development of endosperm starch granules; (2) investigate the effects of DS on composition and physicochemical properties of starches; and (3) compare the different responses to DS between soft and hard wheat.
26311190	5	68	theme	swelling	972:979	arg1	power					981:985	the swelling power	968:985	the swelling power	968:985	DS further increased the swelling power and affected pasting properties of both starches.
26311190	4	69	from	surface	904:910	arg1	XM33					941:944	XM33	941:944	XM33	941:944	DS also decreased the percentage of B-type starch granules in NM13 and increased the number of hollows on the surface of A-type starch granules in XM33.
26311190	4	70	theme	granules	844:851	arg1	percentage					816:825	the percentage	812:825	the percentage of B-type starch granules	812:851	DS also decreased the percentage of B-type starch granules in NM13 and increased the number of hollows on the surface of A-type starch granules in XM33.
26311190	3	71	theme	weight	700:705	arg1	ratio					770:774	the 1000-grain weight, total starch and amylose contents, and amylose-to-amylopectin ratio	685:774	the 1000-grain weight, total starch and amylose contents, and amylose-to-amylopectin ratio of both starches	685:791	DS decreased the 1000-grain weight, total starch and amylose contents, and amylose-to-amylopectin ratio of both starches.
26311190	1	72	theme	endosperm	291:299	arg1	granules					308:315	endosperm starch granules	291:315	endosperm starch granules	291:315	BACKGROUND The objectives of this study were to: (1) observe the effects of drought stress (DS) on the structural development of endosperm starch granules; (2) investigate the effects of DS on composition and physicochemical properties of starches; and (3) compare the different responses to DS between soft and hard wheat.
26311190	3	73	theme	contents	733:740	arg1	ratio					770:774	the 1000-grain weight, total starch and amylose contents, and amylose-to-amylopectin ratio	685:774	the 1000-grain weight, total starch and amylose contents, and amylose-to-amylopectin ratio of both starches	685:791	DS decreased the 1000-grain weight, total starch and amylose contents, and amylose-to-amylopectin ratio of both starches.
26311190	8	74	theme	endosperm	1373:1381	arg1	granules					1390:1397	endosperm starch granules	1373:1397	endosperm starch granules	1373:1397	CONCLUSION Overall, DS can affect the development of endosperm starch granules and the physicochemical properties of starches, thus affecting the qualities of the final wheat products.
26311190	0	75	theme	physicochemical	97:111	arg1	properties					113:122	physicochemical properties	97:122	physicochemical properties	97:122	Effect of drought stress on the development of endosperm starch granules and the composition and physicochemical properties of starches from soft and hard wheat.
26311190	1	76	theme	granules	308:315	arg1	development					276:286	the structural development	261:286	the structural development of endosperm starch granules	261:315	BACKGROUND The objectives of this study were to: (1) observe the effects of drought stress (DS) on the structural development of endosperm starch granules; (2) investigate the effects of DS on composition and physicochemical properties of starches; and (3) compare the different responses to DS between soft and hard wheat.
26311190	0	77	dep	composition	81:91	arg1	the					77:79	the	77:79	the	77:79	Effect of drought stress on the development of endosperm starch granules and the composition and physicochemical properties of starches from soft and hard wheat.
26311190	8	78	theme	granules	1390:1397	arg1	properties					1423:1432	the physicochemical properties	1403:1432	the physicochemical properties of starches	1403:1444	CONCLUSION Overall, DS can affect the development of endosperm starch granules and the physicochemical properties of starches, thus affecting the qualities of the final wheat products.
26311190	8	78	theme	granules	1390:1397	arg1	development					1358:1368	the development	1354:1368	the development of endosperm starch granules	1354:1397	CONCLUSION Overall, DS can affect the development of endosperm starch granules and the physicochemical properties of starches, thus affecting the qualities of the final wheat products.
26311190	1	79	theme	soft	465:468	arg1	wheat					479:483	soft and hard wheat	465:483	soft and hard wheat	465:483	BACKGROUND The objectives of this study were to: (1) observe the effects of drought stress (DS) on the structural development of endosperm starch granules; (2) investigate the effects of DS on composition and physicochemical properties of starches; and (3) compare the different responses to DS between soft and hard wheat.
26311190	1	80	theme	hard	474:477	arg1	wheat					479:483	soft and hard wheat	465:483	soft and hard wheat	465:483	BACKGROUND The objectives of this study were to: (1) observe the effects of drought stress (DS) on the structural development of endosperm starch granules; (2) investigate the effects of DS on composition and physicochemical properties of starches; and (3) compare the different responses to DS between soft and hard wheat.
26311190	0	81	theme	stress	18:23	arg1	Effect					0:5	Effect	0:5	Effect of drought stress on the development of endosperm starch granules and the composition and physicochemical properties of starches from soft and hard wheat.	0:160	Effect of drought stress on the development of endosperm starch granules and the composition and physicochemical properties of starches from soft and hard wheat.
26311190	5	82	theme	starches	1027:1034	arg1	properties					1008:1017	pasting properties	1000:1017	pasting properties of both starches	1000:1034	DS further increased the swelling power and affected pasting properties of both starches.
26311190	6	83	theme	starches	1094:1101	arg1	degrees					1083:1089	the hydrolysis degrees	1068:1089	the hydrolysis degrees of starches	1068:1101	DS also significantly enhanced the hydrolysis degrees of starches by pancreatic α-amylase, Aspergillus niger amyloglucosidase, and HCl in NM13.
26311190	4	84	theme	hollows	889:895	arg1	number					879:884	the number	875:884	the number of hollows	875:895	DS also decreased the percentage of B-type starch granules in NM13 and increased the number of hollows on the surface of A-type starch granules in XM33.
26311190	8	85	dep	CONCLUSION	1320:1329	arg1	affect					1347:1352	affect	1347:1352	affect	1347:1352	CONCLUSION Overall, DS can affect the development of endosperm starch granules and the physicochemical properties of starches, thus affecting the qualities of the final wheat products.
26311190	8	86	theme	starches	1437:1444	arg1	properties					1423:1432	the physicochemical properties	1403:1432	the physicochemical properties of starches	1403:1444	CONCLUSION Overall, DS can affect the development of endosperm starch granules and the physicochemical properties of starches, thus affecting the qualities of the final wheat products.
26311190	8	86	theme	starches	1437:1444	arg1	development					1358:1368	the development	1354:1368	the development of endosperm starch granules	1354:1397	CONCLUSION Overall, DS can affect the development of endosperm starch granules and the physicochemical properties of starches, thus affecting the qualities of the final wheat products.
26311190	5	87	theme	pasting	1000:1006	arg1	properties					1008:1017	pasting properties	1000:1017	pasting properties of both starches	1000:1034	DS further increased the swelling power and affected pasting properties of both starches.
26311190	1	88	dep	BACKGROUND	162:171	arg1	objectives					177:186	The objectives	173:186	BACKGROUND The objectives of this study	162:200	BACKGROUND The objectives of this study were to: (1) observe the effects of drought stress (DS) on the structural development of endosperm starch granules; (2) investigate the effects of DS on composition and physicochemical properties of starches; and (3) compare the different responses to DS between soft and hard wheat.
26311190	4	89	theme	starch	922:927	arg1	granules					929:936	A-type starch granules	915:936	A-type starch granules in XM33	915:944	DS also decreased the percentage of B-type starch granules in NM13 and increased the number of hollows on the surface of A-type starch granules in XM33.
26311190	2	90	theme	wheat	655:659	arg1	cultivars					661:669	the two wheat cultivars	647:669	the two wheat cultivars	647:669	RESULTS DS resulted in large A-type starch granules at 12 d after anthesis (DAA) and a high percentage of B-type starch granules at 18 DAA in endosperm cells of the two wheat cultivars.
27842810	6	0	theme	desirability	954:965	arg1	functions					967:975	the desirability functions	950:975	the desirability functions	950:975	Optimal conditions within the studied range were determined for each extraction method and species based on the desirability functions.
27842810	3	1	theme	obtained	540:547	arg1	extracts					549:556	the obtained extracts	536:556	the obtained extracts	536:556	Total carbohydrate content of the obtained extracts and polysaccharide yields were the variable responses investigated, while temperature and extraction time were the experimental factors.
27842810	1	2	theme	active	274:279	arg1	β-glucans					281:289	biologically active β-glucans	261:289	particularly biologically active β-glucans	248:289	Microwave-assisted extraction (MAE) and pressurized liquid extraction (PLE) were compared as advanced technologies to obtain polysaccharides (particularly biologically active β-glucans) from Pleurotus ostreatus and Ganoderma lucidum fruiting bodies.
27842810	8	3	theme	polysaccharide	1260:1273	arg1	extraction					1275:1284	polysaccharide extraction	1260:1284	polysaccharide extraction from mushrooms	1260:1299	Both extraction systems were effective for polysaccharide extraction from mushrooms.
27842810	1	4	dep	Pleurotus	297:305	arg1	bodies					348:353	fruiting bodies	339:353	fruiting bodies	339:353	Microwave-assisted extraction (MAE) and pressurized liquid extraction (PLE) were compared as advanced technologies to obtain polysaccharides (particularly biologically active β-glucans) from Pleurotus ostreatus and Ganoderma lucidum fruiting bodies.
27842810	1	4	dep	Pleurotus	297:305	arg1	ostreatus					307:315	ostreatus	307:315	ostreatus	307:315	Microwave-assisted extraction (MAE) and pressurized liquid extraction (PLE) were compared as advanced technologies to obtain polysaccharides (particularly biologically active β-glucans) from Pleurotus ostreatus and Ganoderma lucidum fruiting bodies.
27842810	8	5	from	mushrooms	1291:1299	arg1	extraction					1275:1284	polysaccharide extraction	1260:1284	polysaccharide extraction from mushrooms	1260:1299	Both extraction systems were effective for polysaccharide extraction from mushrooms.
27842810	3	6	theme	extracts	549:556	arg1	content					525:531	Total carbohydrate content	506:531	Total carbohydrate content of the obtained extracts and polysaccharide yields	506:582	Total carbohydrate content of the obtained extracts and polysaccharide yields were the variable responses investigated, while temperature and extraction time were the experimental factors.
27842810	3	6	theme	extracts	549:556	arg1	responses					602:610	the variable responses	589:610	the variable responses investigated	589:623	Total carbohydrate content of the obtained extracts and polysaccharide yields were the variable responses investigated, while temperature and extraction time were the experimental factors.
27842810	1	7	from	lucidum	331:337	arg1	polysaccharides					231:245	polysaccharides	231:245	polysaccharides (particularly biologically active β-glucans) from Pleurotus ostreatus and Ganoderma lucidum fruiting bodies	231:353	Microwave-assisted extraction (MAE) and pressurized liquid extraction (PLE) were compared as advanced technologies to obtain polysaccharides (particularly biologically active β-glucans) from Pleurotus ostreatus and Ganoderma lucidum fruiting bodies.
27842810	5	8	theme	MAE	813:815	arg1	extractions					829:839	MAE but not PLE extractions	813:839	MAE but not PLE extractions	813:839	The latter factor slightly affected MAE but not PLE extractions.
27842810	3	9	theme	variable	593:600	arg1	content					525:531	Total carbohydrate content	506:531	Total carbohydrate content of the obtained extracts and polysaccharide yields	506:582	Total carbohydrate content of the obtained extracts and polysaccharide yields were the variable responses investigated, while temperature and extraction time were the experimental factors.
27842810	3	9	theme	variable	593:600	arg1	responses					602:610	the variable responses	589:610	the variable responses investigated	589:623	Total carbohydrate content of the obtained extracts and polysaccharide yields were the variable responses investigated, while temperature and extraction time were the experimental factors.
27842810	2	10	theme	Extraction	356:365	arg1	effectiveness					367:379	Extraction effectiveness	356:379	Extraction effectiveness	356:379	Extraction effectiveness was compared by a full-factorial experimental design (response surface methodology, RSM), using water as extraction solvent.
27842810	3	11	theme	Total	506:510	arg1	content					525:531	Total carbohydrate content	506:531	Total carbohydrate content of the obtained extracts and polysaccharide yields	506:582	Total carbohydrate content of the obtained extracts and polysaccharide yields were the variable responses investigated, while temperature and extraction time were the experimental factors.
27842810	3	11	theme	Total	506:510	arg1	responses					602:610	the variable responses	589:610	the variable responses investigated	589:623	Total carbohydrate content of the obtained extracts and polysaccharide yields were the variable responses investigated, while temperature and extraction time were the experimental factors.
27842810	3	12	theme	yields	577:582	arg1	content					525:531	Total carbohydrate content	506:531	Total carbohydrate content of the obtained extracts and polysaccharide yields	506:582	Total carbohydrate content of the obtained extracts and polysaccharide yields were the variable responses investigated, while temperature and extraction time were the experimental factors.
27842810	3	12	theme	yields	577:582	arg1	responses					602:610	the variable responses	589:610	the variable responses investigated	589:623	Total carbohydrate content of the obtained extracts and polysaccharide yields were the variable responses investigated, while temperature and extraction time were the experimental factors.
27842810	2	13	theme	surface	444:450	arg1	RSM					465:467	RSM	465:467	RSM	465:467	Extraction effectiveness was compared by a full-factorial experimental design (response surface methodology, RSM), using water as extraction solvent.
27842810	2	13	theme	surface	444:450	arg1	methodology					452:462	response surface methodology	435:462	response surface methodology	435:462	Extraction effectiveness was compared by a full-factorial experimental design (response surface methodology, RSM), using water as extraction solvent.
27842810	0	14	theme	extractions	56:66	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of microwave-assisted and pressurized liquid extractions	0:66	Evaluation of microwave-assisted and pressurized liquid extractions to obtain β-d-glucans from mushrooms.
27842810	7	15	theme	NMR	1115:1117	arg1	analyses					1119:1126	NMR analyses	1115:1126	NMR analyses	1115:1126	Regarding the polysaccharide composition, the main differences between the species were more quantitative rather than qualitative, since NMR analyses indicated that all extracts contained mainly β- and α-glucans and heteropolysaccharides.
27842810	6	16	theme	studied	872:878	arg1	range					880:884	the studied range	868:884	the studied range	868:884	Optimal conditions within the studied range were determined for each extraction method and species based on the desirability functions.
27842810	2	17	theme	response	435:442	arg1	RSM					465:467	RSM	465:467	RSM	465:467	Extraction effectiveness was compared by a full-factorial experimental design (response surface methodology, RSM), using water as extraction solvent.
27842810	2	17	theme	response	435:442	arg1	methodology					452:462	response surface methodology	435:462	response surface methodology	435:462	Extraction effectiveness was compared by a full-factorial experimental design (response surface methodology, RSM), using water as extraction solvent.
27842810	3	18	theme	polysaccharide	562:575	arg1	yields					577:582	polysaccharide yields	562:582	polysaccharide yields	562:582	Total carbohydrate content of the obtained extracts and polysaccharide yields were the variable responses investigated, while temperature and extraction time were the experimental factors.
27842810	1	19	from	Pleurotus	297:305	arg1	polysaccharides					231:245	polysaccharides	231:245	polysaccharides (particularly biologically active β-glucans) from Pleurotus ostreatus and Ganoderma lucidum fruiting bodies	231:353	Microwave-assisted extraction (MAE) and pressurized liquid extraction (PLE) were compared as advanced technologies to obtain polysaccharides (particularly biologically active β-glucans) from Pleurotus ostreatus and Ganoderma lucidum fruiting bodies.
27842810	4	20	theme	polysaccharide	740:753	arg1	extraction					755:764	the polysaccharide extraction	736:764	the polysaccharide extraction	736:764	Temperature showed stronger influence in the polysaccharide extraction than time.
27842810	1	21	theme	Microwave-assisted	106:123	arg1	technologies					208:219	advanced technologies	199:219	advanced technologies	199:219	Microwave-assisted extraction (MAE) and pressurized liquid extraction (PLE) were compared as advanced technologies to obtain polysaccharides (particularly biologically active β-glucans) from Pleurotus ostreatus and Ganoderma lucidum fruiting bodies.
27842810	1	21	theme	Microwave-assisted	106:123	arg1	MAE					137:139	MAE	137:139	MAE	137:139	Microwave-assisted extraction (MAE) and pressurized liquid extraction (PLE) were compared as advanced technologies to obtain polysaccharides (particularly biologically active β-glucans) from Pleurotus ostreatus and Ganoderma lucidum fruiting bodies.
27842810	1	21	theme	Microwave-assisted	106:123	arg1	extraction					125:134	Microwave-assisted extraction	106:134	Microwave-assisted extraction (MAE)	106:140	Microwave-assisted extraction (MAE) and pressurized liquid extraction (PLE) were compared as advanced technologies to obtain polysaccharides (particularly biologically active β-glucans) from Pleurotus ostreatus and Ganoderma lucidum fruiting bodies.
27842810	1	21	theme	Microwave-assisted	106:123	arg1	extraction					165:174	pressurized liquid extraction	146:174	pressurized liquid extraction (PLE)	146:180	Microwave-assisted extraction (MAE) and pressurized liquid extraction (PLE) were compared as advanced technologies to obtain polysaccharides (particularly biologically active β-glucans) from Pleurotus ostreatus and Ganoderma lucidum fruiting bodies.
27842810	7	22	theme	main	1024:1027	arg1	differences					1029:1039	the main differences	1020:1039	the main differences between the species	1020:1059	Regarding the polysaccharide composition, the main differences between the species were more quantitative rather than qualitative, since NMR analyses indicated that all extracts contained mainly β- and α-glucans and heteropolysaccharides.
27842810	7	22	theme	main	1024:1027	arg1	quantitative					1071:1082	quantitative	1071:1082	quantitative	1071:1082	Regarding the polysaccharide composition, the main differences between the species were more quantitative rather than qualitative, since NMR analyses indicated that all extracts contained mainly β- and α-glucans and heteropolysaccharides.
27842810	7	23	contain	contained	1156:1164	arg2	β-					1173:1174	β-	1173:1174	β-	1173:1174	Regarding the polysaccharide composition, the main differences between the species were more quantitative rather than qualitative, since NMR analyses indicated that all extracts contained mainly β- and α-glucans and heteropolysaccharides.
27842810	7	23	contain	contained	1156:1164	arg1	extracts					1147:1154	all extracts	1143:1154	all extracts	1143:1154	Regarding the polysaccharide composition, the main differences between the species were more quantitative rather than qualitative, since NMR analyses indicated that all extracts contained mainly β- and α-glucans and heteropolysaccharides.
27842810	7	23	contain	contained	1156:1164	arg2	α-glucans					1180:1188	α-glucans	1180:1188	α-glucans	1180:1188	Regarding the polysaccharide composition, the main differences between the species were more quantitative rather than qualitative, since NMR analyses indicated that all extracts contained mainly β- and α-glucans and heteropolysaccharides.
27842810	1	24	theme	advanced	199:206	arg1	technologies					208:219	advanced technologies	199:219	advanced technologies	199:219	Microwave-assisted extraction (MAE) and pressurized liquid extraction (PLE) were compared as advanced technologies to obtain polysaccharides (particularly biologically active β-glucans) from Pleurotus ostreatus and Ganoderma lucidum fruiting bodies.
27842810	1	24	theme	advanced	199:206	arg1	extraction					125:134	Microwave-assisted extraction	106:134	Microwave-assisted extraction (MAE)	106:140	Microwave-assisted extraction (MAE) and pressurized liquid extraction (PLE) were compared as advanced technologies to obtain polysaccharides (particularly biologically active β-glucans) from Pleurotus ostreatus and Ganoderma lucidum fruiting bodies.
27842810	1	24	theme	advanced	199:206	arg1	extraction					165:174	pressurized liquid extraction	146:174	pressurized liquid extraction (PLE)	146:180	Microwave-assisted extraction (MAE) and pressurized liquid extraction (PLE) were compared as advanced technologies to obtain polysaccharides (particularly biologically active β-glucans) from Pleurotus ostreatus and Ganoderma lucidum fruiting bodies.
27842810	0	25	theme	microwave-assisted	14:31	arg1	extractions					56:66	microwave-assisted and pressurized liquid extractions	14:66	microwave-assisted and pressurized liquid extractions	14:66	Evaluation of microwave-assisted and pressurized liquid extractions to obtain β-d-glucans from mushrooms.
27842810	5	26	theme	latter	781:786	arg1	factor					788:793	The latter factor	777:793	The latter factor	777:793	The latter factor slightly affected MAE but not PLE extractions.
27842810	0	27	from	mushrooms	95:103	arg1	β-d-glucans					78:88	β-d-glucans	78:88	β-d-glucans from mushrooms	78:103	Evaluation of microwave-assisted and pressurized liquid extractions to obtain β-d-glucans from mushrooms.
27842810	1	28	theme	liquid	158:163	arg1	extraction					165:174	pressurized liquid extraction	146:174	pressurized liquid extraction (PLE)	146:180	Microwave-assisted extraction (MAE) and pressurized liquid extraction (PLE) were compared as advanced technologies to obtain polysaccharides (particularly biologically active β-glucans) from Pleurotus ostreatus and Ganoderma lucidum fruiting bodies.
27842810	1	28	theme	liquid	158:163	arg1	PLE					177:179	PLE	177:179	PLE	177:179	Microwave-assisted extraction (MAE) and pressurized liquid extraction (PLE) were compared as advanced technologies to obtain polysaccharides (particularly biologically active β-glucans) from Pleurotus ostreatus and Ganoderma lucidum fruiting bodies.
27842810	1	28	theme	liquid	158:163	arg1	extraction					125:134	Microwave-assisted extraction	106:134	Microwave-assisted extraction (MAE)	106:140	Microwave-assisted extraction (MAE) and pressurized liquid extraction (PLE) were compared as advanced technologies to obtain polysaccharides (particularly biologically active β-glucans) from Pleurotus ostreatus and Ganoderma lucidum fruiting bodies.
27842810	1	28	theme	liquid	158:163	arg1	technologies					208:219	advanced technologies	199:219	advanced technologies	199:219	Microwave-assisted extraction (MAE) and pressurized liquid extraction (PLE) were compared as advanced technologies to obtain polysaccharides (particularly biologically active β-glucans) from Pleurotus ostreatus and Ganoderma lucidum fruiting bodies.
27842810	0	29	theme	liquid	49:54	arg1	extractions					56:66	microwave-assisted and pressurized liquid extractions	14:66	microwave-assisted and pressurized liquid extractions	14:66	Evaluation of microwave-assisted and pressurized liquid extractions to obtain β-d-glucans from mushrooms.
27842810	1	30	theme	Ganoderma	321:329	arg1	lucidum					331:337	Ganoderma lucidum	321:337	Ganoderma lucidum	321:337	Microwave-assisted extraction (MAE) and pressurized liquid extraction (PLE) were compared as advanced technologies to obtain polysaccharides (particularly biologically active β-glucans) from Pleurotus ostreatus and Ganoderma lucidum fruiting bodies.
27842810	3	31	theme	extraction	648:657	arg1	temperature					632:642	temperature	632:642	temperature	632:642	Total carbohydrate content of the obtained extracts and polysaccharide yields were the variable responses investigated, while temperature and extraction time were the experimental factors.
27842810	3	31	theme	extraction	648:657	arg1	time					659:662	extraction time	648:662	extraction time	648:662	Total carbohydrate content of the obtained extracts and polysaccharide yields were the variable responses investigated, while temperature and extraction time were the experimental factors.
27842810	3	31	theme	extraction	648:657	arg1	factors					686:692	the experimental factors	669:692	the experimental factors	669:692	Total carbohydrate content of the obtained extracts and polysaccharide yields were the variable responses investigated, while temperature and extraction time were the experimental factors.
27842810	4	32	theme	stronger	714:721	arg1	influence					723:731	stronger influence	714:731	stronger influence	714:731	Temperature showed stronger influence in the polysaccharide extraction than time.
27842810	0	33	theme	pressurized	37:47	arg1	extractions					56:66	microwave-assisted and pressurized liquid extractions	14:66	microwave-assisted and pressurized liquid extractions	14:66	Evaluation of microwave-assisted and pressurized liquid extractions to obtain β-d-glucans from mushrooms.
27842810	1	34	dep	polysaccharides	231:245	arg1	β-glucans					281:289	biologically active β-glucans	261:289	particularly biologically active β-glucans	248:289	Microwave-assisted extraction (MAE) and pressurized liquid extraction (PLE) were compared as advanced technologies to obtain polysaccharides (particularly biologically active β-glucans) from Pleurotus ostreatus and Ganoderma lucidum fruiting bodies.
27842810	6	35	theme	Optimal	842:848	arg1	conditions					850:859	Optimal conditions	842:859	Optimal conditions within the studied range	842:884	Optimal conditions within the studied range were determined for each extraction method and species based on the desirability functions.
27842810	1	36	theme	fruiting	339:346	arg1	bodies					348:353	fruiting bodies	339:353	fruiting bodies	339:353	Microwave-assisted extraction (MAE) and pressurized liquid extraction (PLE) were compared as advanced technologies to obtain polysaccharides (particularly biologically active β-glucans) from Pleurotus ostreatus and Ganoderma lucidum fruiting bodies.
27842810	8	37	theme	extraction	1222:1231	arg1	systems					1233:1239	Both extraction systems	1217:1239	Both extraction systems	1217:1239	Both extraction systems were effective for polysaccharide extraction from mushrooms.
27842810	2	38	theme	extraction	486:495	arg1	solvent					497:503	extraction solvent	486:503	extraction solvent	486:503	Extraction effectiveness was compared by a full-factorial experimental design (response surface methodology, RSM), using water as extraction solvent.
27842810	3	39	theme	experimental	673:684	arg1	temperature					632:642	temperature	632:642	temperature	632:642	Total carbohydrate content of the obtained extracts and polysaccharide yields were the variable responses investigated, while temperature and extraction time were the experimental factors.
27842810	3	39	theme	experimental	673:684	arg1	time					659:662	extraction time	648:662	extraction time	648:662	Total carbohydrate content of the obtained extracts and polysaccharide yields were the variable responses investigated, while temperature and extraction time were the experimental factors.
27842810	3	39	theme	experimental	673:684	arg1	factors					686:692	the experimental factors	669:692	the experimental factors	669:692	Total carbohydrate content of the obtained extracts and polysaccharide yields were the variable responses investigated, while temperature and extraction time were the experimental factors.
27842810	3	40	theme	carbohydrate	512:523	arg1	content					525:531	Total carbohydrate content	506:531	Total carbohydrate content of the obtained extracts and polysaccharide yields	506:582	Total carbohydrate content of the obtained extracts and polysaccharide yields were the variable responses investigated, while temperature and extraction time were the experimental factors.
27842810	3	40	theme	carbohydrate	512:523	arg1	responses					602:610	the variable responses	589:610	the variable responses investigated	589:623	Total carbohydrate content of the obtained extracts and polysaccharide yields were the variable responses investigated, while temperature and extraction time were the experimental factors.
27842810	6	41	theme	extraction	911:920	arg1	method					922:927	each extraction method	906:927	each extraction method	906:927	Optimal conditions within the studied range were determined for each extraction method and species based on the desirability functions.
27842810	2	42	dep	design	427:432	arg1	RSM					465:467	RSM	465:467	RSM	465:467	Extraction effectiveness was compared by a full-factorial experimental design (response surface methodology, RSM), using water as extraction solvent.
27842810	2	42	dep	design	427:432	arg1	methodology					452:462	response surface methodology	435:462	response surface methodology	435:462	Extraction effectiveness was compared by a full-factorial experimental design (response surface methodology, RSM), using water as extraction solvent.
27842810	1	43	theme	pressurized	146:156	arg1	extraction					165:174	pressurized liquid extraction	146:174	pressurized liquid extraction (PLE)	146:180	Microwave-assisted extraction (MAE) and pressurized liquid extraction (PLE) were compared as advanced technologies to obtain polysaccharides (particularly biologically active β-glucans) from Pleurotus ostreatus and Ganoderma lucidum fruiting bodies.
27842810	1	43	theme	pressurized	146:156	arg1	PLE					177:179	PLE	177:179	PLE	177:179	Microwave-assisted extraction (MAE) and pressurized liquid extraction (PLE) were compared as advanced technologies to obtain polysaccharides (particularly biologically active β-glucans) from Pleurotus ostreatus and Ganoderma lucidum fruiting bodies.
27842810	1	43	theme	pressurized	146:156	arg1	extraction					125:134	Microwave-assisted extraction	106:134	Microwave-assisted extraction (MAE)	106:140	Microwave-assisted extraction (MAE) and pressurized liquid extraction (PLE) were compared as advanced technologies to obtain polysaccharides (particularly biologically active β-glucans) from Pleurotus ostreatus and Ganoderma lucidum fruiting bodies.
27842810	1	43	theme	pressurized	146:156	arg1	technologies					208:219	advanced technologies	199:219	advanced technologies	199:219	Microwave-assisted extraction (MAE) and pressurized liquid extraction (PLE) were compared as advanced technologies to obtain polysaccharides (particularly biologically active β-glucans) from Pleurotus ostreatus and Ganoderma lucidum fruiting bodies.
27842810	5	44	theme	PLE	825:827	arg1	extractions					829:839	MAE but not PLE extractions	813:839	MAE but not PLE extractions	813:839	The latter factor slightly affected MAE but not PLE extractions.
27842810	2	45	theme	experimental	414:425	arg1	design					427:432	a full-factorial experimental design	397:432	a full-factorial experimental design (response surface methodology, RSM)	397:468	Extraction effectiveness was compared by a full-factorial experimental design (response surface methodology, RSM), using water as extraction solvent.
27842810	7	46	theme	polysaccharide	992:1005	arg1	composition					1007:1017	the polysaccharide composition	988:1017	the polysaccharide composition	988:1017	Regarding the polysaccharide composition, the main differences between the species were more quantitative rather than qualitative, since NMR analyses indicated that all extracts contained mainly β- and α-glucans and heteropolysaccharides.
27842810	2	47	theme	full-factorial	399:412	arg1	design					427:432	a full-factorial experimental design	397:432	a full-factorial experimental design (response surface methodology, RSM)	397:468	Extraction effectiveness was compared by a full-factorial experimental design (response surface methodology, RSM), using water as extraction solvent.
27127066	0	0	theme	PLGA	92:95	arg1	composite					97:105	PLGA composite	92:105	PLGA composite	92:105	Osteogenic activity of cyclodextrin-encapsulated doxycycline in a calcium phosphate PCL and PLGA composite.
27127066	3	1	theme	cell	673:676	arg1	cultures					678:685	osteoblastic cell cultures	660:685	osteoblastic cell cultures	660:685	Composites of polycaprolactone (PCL), poly(lactic-co-glycolic acid) (PLGA), and a bioceramic of biphasic calcium phosphate Osteosynt® (BCP) were loaded with DOX encapsulated in β-cyclodextrin (βCD) and were evaluated for effects on osteoblastic cell cultures.
27127066	9	2	theme	nodules	1583:1589	arg1	formation					1555:1563	strong formation	1548:1563	strong formation of mineralization nodules	1548:1589	These results were corroborated by Von Kossa test results, which showed strong formation of mineralization nodules during the same time period.
27127066	2	3	theme	antimicrobial	358:370	arg1	action					372:377	antimicrobial action	358:377	antimicrobial action	358:377	When associated with the broad-spectrum antibiotic doxycycline (DOX), they could favor antimicrobial action and enhance the action of osteogenic composites.
27127066	1	4	theme	bone	252:255	arg1	regeneration					257:268	bone regeneration	252:268	bone regeneration	252:268	Composites of biodegradable polymers and calcium phosphate are bioactive and flexible, and have been proposed for use in tissue engineering and bone regeneration.
27127066	7	5	theme	cell	1169:1172	arg1	proliferation					1174:1186	increased cell proliferation	1159:1186	increased cell proliferation (p<0.05)	1159:1195	Osteoblasts exposed to the composite containing 25μg/mL DOX/βCD had increased cell proliferation (p<0.05) compared to control osteoblast cultures at all experimental time points, reaching a maximum in the second week.
27127066	7	5	theme	cell	1169:1172	arg1	p<0.05					1189:1194	p<0.05	1189:1194	p<0.05	1189:1194	Osteoblasts exposed to the composite containing 25μg/mL DOX/βCD had increased cell proliferation (p<0.05) compared to control osteoblast cultures at all experimental time points, reaching a maximum in the second week.
27127066	0	6	from	activity	11:18	arg1	PCL					84:86	a calcium phosphate PCL	64:86	a calcium phosphate PCL	64:86	Osteogenic activity of cyclodextrin-encapsulated doxycycline in a calcium phosphate PCL and PLGA composite.
27127066	0	6	from	activity	11:18	arg1	composite					97:105	PLGA composite	92:105	PLGA composite	92:105	Osteogenic activity of cyclodextrin-encapsulated doxycycline in a calcium phosphate PCL and PLGA composite.
27127066	10	7	theme	suitable	1696:1703	arg1	sizes					1690:1694	pore sizes	1685:1694	pore sizes suitable for growing osteoblasts	1685:1727	SEM of the composite material revealed a surface topography with pore sizes suitable for growing osteoblasts.
27127066	11	8	theme	DOX/βCD-containing	1842:1859	arg1	composite					1873:1881	a DOX/βCD-containing BCP ceramic composite	1840:1881	a DOX/βCD-containing BCP ceramic composite	1840:1881	Together, these results suggest that osteoblasts are viable, proliferative, and osteogenic in the presence of a DOX/βCD-containing BCP ceramic composite.
27127066	2	9	theme	broad-spectrum	296:309	arg1	doxycycline					322:332	the broad-spectrum antibiotic doxycycline	292:332	the broad-spectrum antibiotic doxycycline (DOX)	292:338	When associated with the broad-spectrum antibiotic doxycycline (DOX), they could favor antimicrobial action and enhance the action of osteogenic composites.
27127066	2	9	theme	broad-spectrum	296:309	arg1	DOX					335:337	DOX	335:337	DOX	335:337	When associated with the broad-spectrum antibiotic doxycycline (DOX), they could favor antimicrobial action and enhance the action of osteogenic composites.
27127066	3	10	theme	phosphate	541:549	arg1	BCP					563:565	BCP	563:565	BCP	563:565	Composites of polycaprolactone (PCL), poly(lactic-co-glycolic acid) (PLGA), and a bioceramic of biphasic calcium phosphate Osteosynt® (BCP) were loaded with DOX encapsulated in β-cyclodextrin (βCD) and were evaluated for effects on osteoblastic cell cultures.
27127066	3	10	theme	phosphate	541:549	arg1	Osteosynt®					551:560	biphasic calcium phosphate Osteosynt®	524:560	biphasic calcium phosphate Osteosynt® (BCP)	524:566	Composites of polycaprolactone (PCL), poly(lactic-co-glycolic acid) (PLGA), and a bioceramic of biphasic calcium phosphate Osteosynt® (BCP) were loaded with DOX encapsulated in β-cyclodextrin (βCD) and were evaluated for effects on osteoblastic cell cultures.
27127066	10	11	theme	composite	1631:1639	arg1	material					1641:1648	the composite material	1627:1648	the composite material	1627:1648	SEM of the composite material revealed a surface topography with pore sizes suitable for growing osteoblasts.
27127066	5	12	theme	phosphatase	884:894	arg1	activity					901:908	alkaline phosphatase (AP) activity	875:908	alkaline phosphatase (AP) activity	875:908	Osteoblast viability was assessed with methyl tetrazolium (MTT) assays after 1day, 7day, and 14days of composite exposure; alkaline phosphatase (AP) activity and collagen production were evaluated after 7days and 14days, and mineral nodule formation after 14days.
27127066	9	13	theme	test	1521:1524	arg1	results					1526:1532	Von Kossa test results	1511:1532	Von Kossa test results	1511:1532	These results were corroborated by Von Kossa test results, which showed strong formation of mineralization nodules during the same time period.
27127066	9	14	theme	same	1602:1605	arg1	period					1612:1617	the same time period	1598:1617	the same time period	1598:1617	These results were corroborated by Von Kossa test results, which showed strong formation of mineralization nodules during the same time period.
27127066	3	15	theme	biphasic	524:531	arg1	BCP					563:565	BCP	563:565	BCP	563:565	Composites of polycaprolactone (PCL), poly(lactic-co-glycolic acid) (PLGA), and a bioceramic of biphasic calcium phosphate Osteosynt® (BCP) were loaded with DOX encapsulated in β-cyclodextrin (βCD) and were evaluated for effects on osteoblastic cell cultures.
27127066	3	15	theme	biphasic	524:531	arg1	Osteosynt®					551:560	biphasic calcium phosphate Osteosynt®	524:560	biphasic calcium phosphate Osteosynt® (BCP)	524:566	Composites of polycaprolactone (PCL), poly(lactic-co-glycolic acid) (PLGA), and a bioceramic of biphasic calcium phosphate Osteosynt® (BCP) were loaded with DOX encapsulated in β-cyclodextrin (βCD) and were evaluated for effects on osteoblastic cell cultures.
27127066	5	16	theme	alkaline	875:882	arg1	AP					897:898	AP	897:898	AP	897:898	Osteoblast viability was assessed with methyl tetrazolium (MTT) assays after 1day, 7day, and 14days of composite exposure; alkaline phosphatase (AP) activity and collagen production were evaluated after 7days and 14days, and mineral nodule formation after 14days.
27127066	5	16	theme	alkaline	875:882	arg1	phosphatase					884:894	alkaline phosphatase	875:894	alkaline phosphatase (AP) activity	875:908	Osteoblast viability was assessed with methyl tetrazolium (MTT) assays after 1day, 7day, and 14days of composite exposure; alkaline phosphatase (AP) activity and collagen production were evaluated after 7days and 14days, and mineral nodule formation after 14days.
27127066	7	17	theme	25μg/mL	1139:1145	arg1	DOX/βCD					1147:1153	the composite containing 25μg/mL DOX/βCD	1114:1153	the composite containing 25μg/mL DOX/βCD	1114:1153	Osteoblasts exposed to the composite containing 25μg/mL DOX/βCD had increased cell proliferation (p<0.05) compared to control osteoblast cultures at all experimental time points, reaching a maximum in the second week.
27127066	5	18	theme	composite	855:863	arg1	exposure					865:872	composite exposure	855:872	composite exposure	855:872	Osteoblast viability was assessed with methyl tetrazolium (MTT) assays after 1day, 7day, and 14days of composite exposure; alkaline phosphatase (AP) activity and collagen production were evaluated after 7days and 14days, and mineral nodule formation after 14days.
27127066	9	19	theme	time	1607:1610	arg1	period					1612:1617	the same time period	1598:1617	the same time period	1598:1617	These results were corroborated by Von Kossa test results, which showed strong formation of mineralization nodules during the same time period.
27127066	5	20	theme	collagen	914:921	arg1	production					923:932	collagen production	914:932	collagen production	914:932	Osteoblast viability was assessed with methyl tetrazolium (MTT) assays after 1day, 7day, and 14days of composite exposure; alkaline phosphatase (AP) activity and collagen production were evaluated after 7days and 14days, and mineral nodule formation after 14days.
27127066	11	21	theme	ceramic	1865:1871	arg1	composite					1873:1881	a DOX/βCD-containing BCP ceramic composite	1840:1881	a DOX/βCD-containing BCP ceramic composite	1840:1881	Together, these results suggest that osteoblasts are viable, proliferative, and osteogenic in the presence of a DOX/βCD-containing BCP ceramic composite.
27127066	11	22	from	viable	1783:1788	arg1	presence					1828:1835	the presence	1824:1835	the presence of a DOX/βCD-containing BCP ceramic composite	1824:1881	Together, these results suggest that osteoblasts are viable, proliferative, and osteogenic in the presence of a DOX/βCD-containing BCP ceramic composite.
27127066	3	23	theme	bioceramic	510:519	arg1	poly					466:469	poly	466:469	poly(lactic-co-glycolic acid) (PLGA)	466:501	Composites of polycaprolactone (PCL), poly(lactic-co-glycolic acid) (PLGA), and a bioceramic of biphasic calcium phosphate Osteosynt® (BCP) were loaded with DOX encapsulated in β-cyclodextrin (βCD) and were evaluated for effects on osteoblastic cell cultures.
27127066	3	23	theme	bioceramic	510:519	arg1	bioceramic					510:519	a bioceramic	508:519	a bioceramic of biphasic calcium phosphate Osteosynt® (BCP)	508:566	Composites of polycaprolactone (PCL), poly(lactic-co-glycolic acid) (PLGA), and a bioceramic of biphasic calcium phosphate Osteosynt® (BCP) were loaded with DOX encapsulated in β-cyclodextrin (βCD) and were evaluated for effects on osteoblastic cell cultures.
27127066	3	23	theme	bioceramic	510:519	arg1	polycaprolactone					442:457	polycaprolactone	442:457	polycaprolactone (PCL)	442:463	Composites of polycaprolactone (PCL), poly(lactic-co-glycolic acid) (PLGA), and a bioceramic of biphasic calcium phosphate Osteosynt® (BCP) were loaded with DOX encapsulated in β-cyclodextrin (βCD) and were evaluated for effects on osteoblastic cell cultures.
27127066	3	23	theme	bioceramic	510:519	arg1	Composites					428:437	Composites	428:437	Composites of polycaprolactone (PCL), poly(lactic-co-glycolic acid) (PLGA), and a bioceramic of biphasic calcium phosphate Osteosynt® (BCP)	428:566	Composites of polycaprolactone (PCL), poly(lactic-co-glycolic acid) (PLGA), and a bioceramic of biphasic calcium phosphate Osteosynt® (BCP) were loaded with DOX encapsulated in β-cyclodextrin (βCD) and were evaluated for effects on osteoblastic cell cultures.
27127066	6	24	theme	Composite	1016:1024	arg1	structures					1026:1035	Composite structures	1016:1035	Composite structures	1016:1035	Composite structures were evaluated by scanning electron microscopy (SEM).
27127066	7	25	theme	composite	1118:1126	arg1	DOX/βCD					1147:1153	the composite containing 25μg/mL DOX/βCD	1114:1153	the composite containing 25μg/mL DOX/βCD	1114:1153	Osteoblasts exposed to the composite containing 25μg/mL DOX/βCD had increased cell proliferation (p<0.05) compared to control osteoblast cultures at all experimental time points, reaching a maximum in the second week.
27127066	7	26	theme	experimental	1244:1255	arg1	points					1262:1267	all experimental time points	1240:1267	all experimental time points	1240:1267	Osteoblasts exposed to the composite containing 25μg/mL DOX/βCD had increased cell proliferation (p<0.05) compared to control osteoblast cultures at all experimental time points, reaching a maximum in the second week.
27127066	3	27	theme	Osteosynt®	551:560	arg1	polycaprolactone					442:457	polycaprolactone	442:457	polycaprolactone (PCL)	442:463	Composites of polycaprolactone (PCL), poly(lactic-co-glycolic acid) (PLGA), and a bioceramic of biphasic calcium phosphate Osteosynt® (BCP) were loaded with DOX encapsulated in β-cyclodextrin (βCD) and were evaluated for effects on osteoblastic cell cultures.
27127066	3	27	theme	Osteosynt®	551:560	arg1	PCL					460:462	PCL	460:462	PCL	460:462	Composites of polycaprolactone (PCL), poly(lactic-co-glycolic acid) (PLGA), and a bioceramic of biphasic calcium phosphate Osteosynt® (BCP) were loaded with DOX encapsulated in β-cyclodextrin (βCD) and were evaluated for effects on osteoblastic cell cultures.
27127066	3	27	theme	Osteosynt®	551:560	arg1	acid					490:493	lactic-co-glycolic acid	471:493	lactic-co-glycolic acid	471:493	Composites of polycaprolactone (PCL), poly(lactic-co-glycolic acid) (PLGA), and a bioceramic of biphasic calcium phosphate Osteosynt® (BCP) were loaded with DOX encapsulated in β-cyclodextrin (βCD) and were evaluated for effects on osteoblastic cell cultures.
27127066	3	27	theme	Osteosynt®	551:560	arg1	bioceramic					510:519	a bioceramic	508:519	a bioceramic of biphasic calcium phosphate Osteosynt® (BCP)	508:566	Composites of polycaprolactone (PCL), poly(lactic-co-glycolic acid) (PLGA), and a bioceramic of biphasic calcium phosphate Osteosynt® (BCP) were loaded with DOX encapsulated in β-cyclodextrin (βCD) and were evaluated for effects on osteoblastic cell cultures.
27127066	3	27	theme	Osteosynt®	551:560	arg1	poly					466:469	poly	466:469	poly(lactic-co-glycolic acid) (PLGA)	466:501	Composites of polycaprolactone (PCL), poly(lactic-co-glycolic acid) (PLGA), and a bioceramic of biphasic calcium phosphate Osteosynt® (BCP) were loaded with DOX encapsulated in β-cyclodextrin (βCD) and were evaluated for effects on osteoblastic cell cultures.
27127066	3	27	theme	Osteosynt®	551:560	arg1	PLGA					497:500	PLGA	497:500	PLGA	497:500	Composites of polycaprolactone (PCL), poly(lactic-co-glycolic acid) (PLGA), and a bioceramic of biphasic calcium phosphate Osteosynt® (BCP) were loaded with DOX encapsulated in β-cyclodextrin (βCD) and were evaluated for effects on osteoblastic cell cultures.
27127066	11	28	theme	BCP	1861:1863	arg1	composite					1873:1881	a DOX/βCD-containing BCP ceramic composite	1840:1881	a DOX/βCD-containing BCP ceramic composite	1840:1881	Together, these results suggest that osteoblasts are viable, proliferative, and osteogenic in the presence of a DOX/βCD-containing BCP ceramic composite.
27127066	0	29	theme	Osteogenic	0:9	arg1	activity					11:18	Osteogenic activity	0:18	Osteogenic activity of cyclodextrin-encapsulated doxycycline in a calcium phosphate PCL and PLGA composite.	0:106	Osteogenic activity of cyclodextrin-encapsulated doxycycline in a calcium phosphate PCL and PLGA composite.
27127066	9	30	theme	Kossa	1515:1519	arg1	results					1526:1532	Von Kossa test results	1511:1532	Von Kossa test results	1511:1532	These results were corroborated by Von Kossa test results, which showed strong formation of mineralization nodules during the same time period.
27127066	8	31	theme	AP	1309:1310	arg1	activity					1312:1319	AP activity	1309:1319	AP activity	1309:1319	AP activity and collagen secretion levels were also elevated in osteoblasts exposed to the DOX/βCD composite (p<0.05 vs. controls) and reached a maximum after 14days.
27127066	4	32	theme	double	730:735	arg1	method					744:749	a double mixing method	728:749	a double mixing method	728:749	The DOX/βCD composite was prepared with a double mixing method.
27127066	3	33	theme	polycaprolactone	442:457	arg1	poly					466:469	poly	466:469	poly(lactic-co-glycolic acid) (PLGA)	466:501	Composites of polycaprolactone (PCL), poly(lactic-co-glycolic acid) (PLGA), and a bioceramic of biphasic calcium phosphate Osteosynt® (BCP) were loaded with DOX encapsulated in β-cyclodextrin (βCD) and were evaluated for effects on osteoblastic cell cultures.
27127066	3	33	theme	polycaprolactone	442:457	arg1	bioceramic					510:519	a bioceramic	508:519	a bioceramic of biphasic calcium phosphate Osteosynt® (BCP)	508:566	Composites of polycaprolactone (PCL), poly(lactic-co-glycolic acid) (PLGA), and a bioceramic of biphasic calcium phosphate Osteosynt® (BCP) were loaded with DOX encapsulated in β-cyclodextrin (βCD) and were evaluated for effects on osteoblastic cell cultures.
27127066	3	33	theme	polycaprolactone	442:457	arg1	polycaprolactone					442:457	polycaprolactone	442:457	polycaprolactone (PCL)	442:463	Composites of polycaprolactone (PCL), poly(lactic-co-glycolic acid) (PLGA), and a bioceramic of biphasic calcium phosphate Osteosynt® (BCP) were loaded with DOX encapsulated in β-cyclodextrin (βCD) and were evaluated for effects on osteoblastic cell cultures.
27127066	3	33	theme	polycaprolactone	442:457	arg1	Composites					428:437	Composites	428:437	Composites of polycaprolactone (PCL), poly(lactic-co-glycolic acid) (PLGA), and a bioceramic of biphasic calcium phosphate Osteosynt® (BCP)	428:566	Composites of polycaprolactone (PCL), poly(lactic-co-glycolic acid) (PLGA), and a bioceramic of biphasic calcium phosphate Osteosynt® (BCP) were loaded with DOX encapsulated in β-cyclodextrin (βCD) and were evaluated for effects on osteoblastic cell cultures.
27127066	0	34	theme	cyclodextrin-encapsulated	23:47	arg1	doxycycline					49:59	cyclodextrin-encapsulated doxycycline	23:59	cyclodextrin-encapsulated doxycycline	23:59	Osteogenic activity of cyclodextrin-encapsulated doxycycline in a calcium phosphate PCL and PLGA composite.
27127066	3	35	theme	calcium	533:539	arg1	BCP					563:565	BCP	563:565	BCP	563:565	Composites of polycaprolactone (PCL), poly(lactic-co-glycolic acid) (PLGA), and a bioceramic of biphasic calcium phosphate Osteosynt® (BCP) were loaded with DOX encapsulated in β-cyclodextrin (βCD) and were evaluated for effects on osteoblastic cell cultures.
27127066	3	35	theme	calcium	533:539	arg1	Osteosynt®					551:560	biphasic calcium phosphate Osteosynt®	524:560	biphasic calcium phosphate Osteosynt® (BCP)	524:566	Composites of polycaprolactone (PCL), poly(lactic-co-glycolic acid) (PLGA), and a bioceramic of biphasic calcium phosphate Osteosynt® (BCP) were loaded with DOX encapsulated in β-cyclodextrin (βCD) and were evaluated for effects on osteoblastic cell cultures.
27127066	3	36	theme	poly	466:469	arg1	poly					466:469	poly	466:469	poly(lactic-co-glycolic acid) (PLGA)	466:501	Composites of polycaprolactone (PCL), poly(lactic-co-glycolic acid) (PLGA), and a bioceramic of biphasic calcium phosphate Osteosynt® (BCP) were loaded with DOX encapsulated in β-cyclodextrin (βCD) and were evaluated for effects on osteoblastic cell cultures.
27127066	3	36	theme	poly	466:469	arg1	bioceramic					510:519	a bioceramic	508:519	a bioceramic of biphasic calcium phosphate Osteosynt® (BCP)	508:566	Composites of polycaprolactone (PCL), poly(lactic-co-glycolic acid) (PLGA), and a bioceramic of biphasic calcium phosphate Osteosynt® (BCP) were loaded with DOX encapsulated in β-cyclodextrin (βCD) and were evaluated for effects on osteoblastic cell cultures.
27127066	3	36	theme	poly	466:469	arg1	polycaprolactone					442:457	polycaprolactone	442:457	polycaprolactone (PCL)	442:463	Composites of polycaprolactone (PCL), poly(lactic-co-glycolic acid) (PLGA), and a bioceramic of biphasic calcium phosphate Osteosynt® (BCP) were loaded with DOX encapsulated in β-cyclodextrin (βCD) and were evaluated for effects on osteoblastic cell cultures.
27127066	3	36	theme	poly	466:469	arg1	Composites					428:437	Composites	428:437	Composites of polycaprolactone (PCL), poly(lactic-co-glycolic acid) (PLGA), and a bioceramic of biphasic calcium phosphate Osteosynt® (BCP)	428:566	Composites of polycaprolactone (PCL), poly(lactic-co-glycolic acid) (PLGA), and a bioceramic of biphasic calcium phosphate Osteosynt® (BCP) were loaded with DOX encapsulated in β-cyclodextrin (βCD) and were evaluated for effects on osteoblastic cell cultures.
27127066	5	37	theme	tetrazolium	798:808	arg1	assays					816:821	methyl tetrazolium (MTT) assays	791:821	methyl tetrazolium (MTT) assays	791:821	Osteoblast viability was assessed with methyl tetrazolium (MTT) assays after 1day, 7day, and 14days of composite exposure; alkaline phosphatase (AP) activity and collagen production were evaluated after 7days and 14days, and mineral nodule formation after 14days.
27127066	10	38	theme	pore	1685:1688	arg1	sizes					1690:1694	pore sizes	1685:1694	pore sizes suitable for growing osteoblasts	1685:1727	SEM of the composite material revealed a surface topography with pore sizes suitable for growing osteoblasts.
27127066	8	39	theme	secretion	1334:1342	arg1	levels					1344:1349	AP activity and collagen secretion levels	1309:1349	AP activity and collagen secretion levels	1309:1349	AP activity and collagen secretion levels were also elevated in osteoblasts exposed to the DOX/βCD composite (p<0.05 vs. controls) and reached a maximum after 14days.
27127066	2	40	theme	antibiotic	311:320	arg1	doxycycline					322:332	the broad-spectrum antibiotic doxycycline	292:332	the broad-spectrum antibiotic doxycycline (DOX)	292:338	When associated with the broad-spectrum antibiotic doxycycline (DOX), they could favor antimicrobial action and enhance the action of osteogenic composites.
27127066	2	40	theme	antibiotic	311:320	arg1	DOX					335:337	DOX	335:337	DOX	335:337	When associated with the broad-spectrum antibiotic doxycycline (DOX), they could favor antimicrobial action and enhance the action of osteogenic composites.
27127066	6	41	theme	scanning	1055:1062	arg1	microscopy					1073:1082	scanning electron microscopy	1055:1082	scanning electron microscopy (SEM)	1055:1088	Composite structures were evaluated by scanning electron microscopy (SEM).
27127066	6	41	theme	scanning	1055:1062	arg1	SEM					1085:1087	SEM	1085:1087	SEM	1085:1087	Composite structures were evaluated by scanning electron microscopy (SEM).
27127066	3	42	from	effects	649:655	arg1	cultures					678:685	osteoblastic cell cultures	660:685	osteoblastic cell cultures	660:685	Composites of polycaprolactone (PCL), poly(lactic-co-glycolic acid) (PLGA), and a bioceramic of biphasic calcium phosphate Osteosynt® (BCP) were loaded with DOX encapsulated in β-cyclodextrin (βCD) and were evaluated for effects on osteoblastic cell cultures.
27127066	5	43	theme	exposure	865:872	arg1	14days					845:850	14days	845:850	14days	845:850	Osteoblast viability was assessed with methyl tetrazolium (MTT) assays after 1day, 7day, and 14days of composite exposure; alkaline phosphatase (AP) activity and collagen production were evaluated after 7days and 14days, and mineral nodule formation after 14days.
27127066	5	43	theme	exposure	865:872	arg1	1day					829:832	1day	829:832	1day	829:832	Osteoblast viability was assessed with methyl tetrazolium (MTT) assays after 1day, 7day, and 14days of composite exposure; alkaline phosphatase (AP) activity and collagen production were evaluated after 7days and 14days, and mineral nodule formation after 14days.
27127066	5	43	theme	exposure	865:872	arg1	7day					835:838	7day	835:838	7day	835:838	Osteoblast viability was assessed with methyl tetrazolium (MTT) assays after 1day, 7day, and 14days of composite exposure; alkaline phosphatase (AP) activity and collagen production were evaluated after 7days and 14days, and mineral nodule formation after 14days.
27127066	7	44	theme	containing	1128:1137	arg1	DOX/βCD					1147:1153	the composite containing 25μg/mL DOX/βCD	1114:1153	the composite containing 25μg/mL DOX/βCD	1114:1153	Osteoblasts exposed to the composite containing 25μg/mL DOX/βCD had increased cell proliferation (p<0.05) compared to control osteoblast cultures at all experimental time points, reaching a maximum in the second week.
27127066	2	45	theme	composites	416:425	arg1	action					395:400	the action	391:400	the action of osteogenic composites	391:425	When associated with the broad-spectrum antibiotic doxycycline (DOX), they could favor antimicrobial action and enhance the action of osteogenic composites.
27127066	0	46	theme	doxycycline	49:59	arg1	activity					11:18	Osteogenic activity	0:18	Osteogenic activity of cyclodextrin-encapsulated doxycycline in a calcium phosphate PCL and PLGA composite.	0:106	Osteogenic activity of cyclodextrin-encapsulated doxycycline in a calcium phosphate PCL and PLGA composite.
27127066	8	47	dep	composite	1408:1416	arg1	p<0.05					1419:1424	p<0.05	1419:1424	p<0.05	1419:1424	AP activity and collagen secretion levels were also elevated in osteoblasts exposed to the DOX/βCD composite (p<0.05 vs. controls) and reached a maximum after 14days.
27127066	8	47	dep	composite	1408:1416	arg1	controls					1430:1437	controls	1430:1437	controls	1430:1437	AP activity and collagen secretion levels were also elevated in osteoblasts exposed to the DOX/βCD composite (p<0.05 vs. controls) and reached a maximum after 14days.
27127066	5	48	theme	Osteoblast	752:761	arg1	viability					763:771	Osteoblast viability	752:771	Osteoblast viability	752:771	Osteoblast viability was assessed with methyl tetrazolium (MTT) assays after 1day, 7day, and 14days of composite exposure; alkaline phosphatase (AP) activity and collagen production were evaluated after 7days and 14days, and mineral nodule formation after 14days.
27127066	5	49	theme	methyl	791:796	arg1	MTT					811:813	MTT	811:813	MTT	811:813	Osteoblast viability was assessed with methyl tetrazolium (MTT) assays after 1day, 7day, and 14days of composite exposure; alkaline phosphatase (AP) activity and collagen production were evaluated after 7days and 14days, and mineral nodule formation after 14days.
27127066	5	49	theme	methyl	791:796	arg1	tetrazolium					798:808	methyl tetrazolium	791:808	methyl tetrazolium (MTT) assays	791:821	Osteoblast viability was assessed with methyl tetrazolium (MTT) assays after 1day, 7day, and 14days of composite exposure; alkaline phosphatase (AP) activity and collagen production were evaluated after 7days and 14days, and mineral nodule formation after 14days.
27127066	2	50	theme	osteogenic	405:414	arg1	composites					416:425	osteogenic composites	405:425	osteogenic composites	405:425	When associated with the broad-spectrum antibiotic doxycycline (DOX), they could favor antimicrobial action and enhance the action of osteogenic composites.
27127066	0	51	theme	calcium	66:72	arg1	PCL					84:86	a calcium phosphate PCL	64:86	a calcium phosphate PCL	64:86	Osteogenic activity of cyclodextrin-encapsulated doxycycline in a calcium phosphate PCL and PLGA composite.
27127066	8	52	theme	activity	1312:1319	arg1	levels					1344:1349	AP activity and collagen secretion levels	1309:1349	AP activity and collagen secretion levels	1309:1349	AP activity and collagen secretion levels were also elevated in osteoblasts exposed to the DOX/βCD composite (p<0.05 vs. controls) and reached a maximum after 14days.
27127066	8	53	theme	DOX/βCD	1400:1406	arg1	composite					1408:1416	the DOX/βCD composite	1396:1416	the DOX/βCD composite (p<0.05 vs. controls)	1396:1438	AP activity and collagen secretion levels were also elevated in osteoblasts exposed to the DOX/βCD composite (p<0.05 vs. controls) and reached a maximum after 14days.
27127066	10	54	theme	material	1641:1648	arg1	SEM					1620:1622	SEM	1620:1622	SEM of the composite material	1620:1648	SEM of the composite material revealed a surface topography with pore sizes suitable for growing osteoblasts.
27127066	1	55	from	use	222:224	arg1	engineering					236:246	tissue engineering	229:246	tissue engineering	229:246	Composites of biodegradable polymers and calcium phosphate are bioactive and flexible, and have been proposed for use in tissue engineering and bone regeneration.
27127066	1	55	from	use	222:224	arg1	regeneration					257:268	bone regeneration	252:268	bone regeneration	252:268	Composites of biodegradable polymers and calcium phosphate are bioactive and flexible, and have been proposed for use in tissue engineering and bone regeneration.
27127066	1	56	theme	biodegradable	122:134	arg1	polymers					136:143	biodegradable polymers	122:143	biodegradable polymers	122:143	Composites of biodegradable polymers and calcium phosphate are bioactive and flexible, and have been proposed for use in tissue engineering and bone regeneration.
27127066	10	57	theme	surface	1661:1667	arg1	topography					1669:1678	a surface topography	1659:1678	a surface topography with pore sizes suitable for growing osteoblasts	1659:1727	SEM of the composite material revealed a surface topography with pore sizes suitable for growing osteoblasts.
27127066	5	58	theme	mineral	977:983	arg1	formation					992:1000	mineral nodule formation	977:1000	mineral nodule formation	977:1000	Osteoblast viability was assessed with methyl tetrazolium (MTT) assays after 1day, 7day, and 14days of composite exposure; alkaline phosphatase (AP) activity and collagen production were evaluated after 7days and 14days, and mineral nodule formation after 14days.
27127066	1	59	theme	polymers	136:143	arg1	Composites					108:117	Composites	108:117	Composites of biodegradable polymers and calcium phosphate	108:165	Composites of biodegradable polymers and calcium phosphate are bioactive and flexible, and have been proposed for use in tissue engineering and bone regeneration.
27127066	1	59	theme	polymers	136:143	arg1	polymers					136:143	biodegradable polymers	122:143	biodegradable polymers	122:143	Composites of biodegradable polymers and calcium phosphate are bioactive and flexible, and have been proposed for use in tissue engineering and bone regeneration.
27127066	1	59	theme	polymers	136:143	arg1	phosphate					157:165	calcium phosphate	149:165	calcium phosphate	149:165	Composites of biodegradable polymers and calcium phosphate are bioactive and flexible, and have been proposed for use in tissue engineering and bone regeneration.
27127066	10	60	theme	growing	1709:1715	arg1	osteoblasts					1717:1727	growing osteoblasts	1709:1727	growing osteoblasts	1709:1727	SEM of the composite material revealed a surface topography with pore sizes suitable for growing osteoblasts.
27127066	9	61	theme	mineralization	1568:1581	arg1	nodules					1583:1589	mineralization nodules	1568:1589	mineralization nodules	1568:1589	These results were corroborated by Von Kossa test results, which showed strong formation of mineralization nodules during the same time period.
27127066	5	62	theme	nodule	985:990	arg1	formation					992:1000	mineral nodule formation	977:1000	mineral nodule formation	977:1000	Osteoblast viability was assessed with methyl tetrazolium (MTT) assays after 1day, 7day, and 14days of composite exposure; alkaline phosphatase (AP) activity and collagen production were evaluated after 7days and 14days, and mineral nodule formation after 14days.
27127066	9	63	theme	strong	1548:1553	arg1	formation					1555:1563	strong formation	1548:1563	strong formation of mineralization nodules	1548:1589	These results were corroborated by Von Kossa test results, which showed strong formation of mineralization nodules during the same time period.
27127066	7	64	contain	had	1155:1157	arg1	Osteoblasts					1091:1101	Osteoblasts	1091:1101	Osteoblasts exposed to the composite containing 25μg/mL DOX/βCD	1091:1153	Osteoblasts exposed to the composite containing 25μg/mL DOX/βCD had increased cell proliferation (p<0.05) compared to control osteoblast cultures at all experimental time points, reaching a maximum in the second week.
27127066	7	64	contain	had	1155:1157	arg2	p<0.05					1189:1194	p<0.05	1189:1194	p<0.05	1189:1194	Osteoblasts exposed to the composite containing 25μg/mL DOX/βCD had increased cell proliferation (p<0.05) compared to control osteoblast cultures at all experimental time points, reaching a maximum in the second week.
27127066	7	64	contain	had	1155:1157	arg2	proliferation					1174:1186	increased cell proliferation	1159:1186	increased cell proliferation (p<0.05)	1159:1195	Osteoblasts exposed to the composite containing 25μg/mL DOX/βCD had increased cell proliferation (p<0.05) compared to control osteoblast cultures at all experimental time points, reaching a maximum in the second week.
27127066	3	65	theme	lactic-co-glycolic	471:488	arg1	poly					466:469	poly	466:469	poly(lactic-co-glycolic acid) (PLGA)	466:501	Composites of polycaprolactone (PCL), poly(lactic-co-glycolic acid) (PLGA), and a bioceramic of biphasic calcium phosphate Osteosynt® (BCP) were loaded with DOX encapsulated in β-cyclodextrin (βCD) and were evaluated for effects on osteoblastic cell cultures.
27127066	3	65	theme	lactic-co-glycolic	471:488	arg1	acid					490:493	lactic-co-glycolic acid	471:493	lactic-co-glycolic acid	471:493	Composites of polycaprolactone (PCL), poly(lactic-co-glycolic acid) (PLGA), and a bioceramic of biphasic calcium phosphate Osteosynt® (BCP) were loaded with DOX encapsulated in β-cyclodextrin (βCD) and were evaluated for effects on osteoblastic cell cultures.
27127066	6	66	theme	electron	1064:1071	arg1	microscopy					1073:1082	scanning electron microscopy	1055:1082	scanning electron microscopy (SEM)	1055:1088	Composite structures were evaluated by scanning electron microscopy (SEM).
27127066	6	66	theme	electron	1064:1071	arg1	SEM					1085:1087	SEM	1085:1087	SEM	1085:1087	Composite structures were evaluated by scanning electron microscopy (SEM).
27127066	4	67	theme	mixing	737:742	arg1	method					744:749	a double mixing method	728:749	a double mixing method	728:749	The DOX/βCD composite was prepared with a double mixing method.
27127066	10	68	with	topography	1669:1678	arg1	sizes					1690:1694	pore sizes	1685:1694	pore sizes suitable for growing osteoblasts	1685:1727	SEM of the composite material revealed a surface topography with pore sizes suitable for growing osteoblasts.
27127066	4	69	theme	DOX/βCD	692:698	arg1	composite					700:708	The DOX/βCD composite	688:708	The DOX/βCD composite	688:708	The DOX/βCD composite was prepared with a double mixing method.
27127066	11	70	from	presence	1828:1835	arg1	viable					1783:1788	viable	1783:1788	viable	1783:1788	Together, these results suggest that osteoblasts are viable, proliferative, and osteogenic in the presence of a DOX/βCD-containing BCP ceramic composite.
27127066	1	71	theme	calcium	149:155	arg1	phosphate					157:165	calcium phosphate	149:165	calcium phosphate	149:165	Composites of biodegradable polymers and calcium phosphate are bioactive and flexible, and have been proposed for use in tissue engineering and bone regeneration.
27127066	9	72	theme	Von	1511:1513	arg1	results					1526:1532	Von Kossa test results	1511:1532	Von Kossa test results	1511:1532	These results were corroborated by Von Kossa test results, which showed strong formation of mineralization nodules during the same time period.
27127066	1	73	theme	tissue	229:234	arg1	engineering					236:246	tissue engineering	229:246	tissue engineering	229:246	Composites of biodegradable polymers and calcium phosphate are bioactive and flexible, and have been proposed for use in tissue engineering and bone regeneration.
27127066	7	74	theme	time	1257:1260	arg1	points					1262:1267	all experimental time points	1240:1267	all experimental time points	1240:1267	Osteoblasts exposed to the composite containing 25μg/mL DOX/βCD had increased cell proliferation (p<0.05) compared to control osteoblast cultures at all experimental time points, reaching a maximum in the second week.
27127066	11	75	theme	composite	1873:1881	arg1	presence					1828:1835	the presence	1824:1835	the presence of a DOX/βCD-containing BCP ceramic composite	1824:1881	Together, these results suggest that osteoblasts are viable, proliferative, and osteogenic in the presence of a DOX/βCD-containing BCP ceramic composite.
27127066	1	76	theme	phosphate	157:165	arg1	Composites					108:117	Composites	108:117	Composites of biodegradable polymers and calcium phosphate	108:165	Composites of biodegradable polymers and calcium phosphate are bioactive and flexible, and have been proposed for use in tissue engineering and bone regeneration.
27127066	1	76	theme	phosphate	157:165	arg1	polymers					136:143	biodegradable polymers	122:143	biodegradable polymers	122:143	Composites of biodegradable polymers and calcium phosphate are bioactive and flexible, and have been proposed for use in tissue engineering and bone regeneration.
27127066	1	76	theme	phosphate	157:165	arg1	phosphate					157:165	calcium phosphate	149:165	calcium phosphate	149:165	Composites of biodegradable polymers and calcium phosphate are bioactive and flexible, and have been proposed for use in tissue engineering and bone regeneration.
27127066	8	77	theme	collagen	1325:1332	arg1	secretion					1334:1342	collagen secretion	1325:1342	collagen secretion	1325:1342	AP activity and collagen secretion levels were also elevated in osteoblasts exposed to the DOX/βCD composite (p<0.05 vs. controls) and reached a maximum after 14days.
27127066	0	78	theme	phosphate	74:82	arg1	PCL					84:86	a calcium phosphate PCL	64:86	a calcium phosphate PCL	64:86	Osteogenic activity of cyclodextrin-encapsulated doxycycline in a calcium phosphate PCL and PLGA composite.
27127066	3	79	theme	osteoblastic	660:671	arg1	cultures					678:685	osteoblastic cell cultures	660:685	osteoblastic cell cultures	660:685	Composites of polycaprolactone (PCL), poly(lactic-co-glycolic acid) (PLGA), and a bioceramic of biphasic calcium phosphate Osteosynt® (BCP) were loaded with DOX encapsulated in β-cyclodextrin (βCD) and were evaluated for effects on osteoblastic cell cultures.
27127066	7	80	theme	second	1296:1301	arg1	week					1303:1306	the second week	1292:1306	the second week	1292:1306	Osteoblasts exposed to the composite containing 25μg/mL DOX/βCD had increased cell proliferation (p<0.05) compared to control osteoblast cultures at all experimental time points, reaching a maximum in the second week.
27127066	7	81	theme	osteoblast	1217:1226	arg1	cultures					1228:1235	control osteoblast cultures	1209:1235	control osteoblast cultures	1209:1235	Osteoblasts exposed to the composite containing 25μg/mL DOX/βCD had increased cell proliferation (p<0.05) compared to control osteoblast cultures at all experimental time points, reaching a maximum in the second week.
27127066	7	82	theme	control	1209:1215	arg1	cultures					1228:1235	control osteoblast cultures	1209:1235	control osteoblast cultures	1209:1235	Osteoblasts exposed to the composite containing 25μg/mL DOX/βCD had increased cell proliferation (p<0.05) compared to control osteoblast cultures at all experimental time points, reaching a maximum in the second week.
27127066	7	83	theme	increased	1159:1167	arg1	proliferation					1174:1186	increased cell proliferation	1159:1186	increased cell proliferation (p<0.05)	1159:1195	Osteoblasts exposed to the composite containing 25μg/mL DOX/βCD had increased cell proliferation (p<0.05) compared to control osteoblast cultures at all experimental time points, reaching a maximum in the second week.
27127066	7	83	theme	increased	1159:1167	arg1	p<0.05					1189:1194	p<0.05	1189:1194	p<0.05	1189:1194	Osteoblasts exposed to the composite containing 25μg/mL DOX/βCD had increased cell proliferation (p<0.05) compared to control osteoblast cultures at all experimental time points, reaching a maximum in the second week.
29150953	2	0	theme	osteoarthritis	336:349	arg1	progression					316:326	the progression	312:326	the progression of knee osteoarthritis	312:349	Currently, there is only one modeling framework which can be applied to predict the progression of knee osteoarthritis but it only considers degenerative changes in the collagen fibril network.
29150953	6	1	theme	relevant	996:1003	arg1	conditions					1014:1023	loading conditions	1006:1023	loading conditions	1006:1023	Models were tested for normal and abnormal, physiologically relevant, loading conditions in the knee.
29150953	7	2	theme	depth-wise	1061:1070	arg1	changes					1086:1092	the predicted depth-wise compositional changes	1047:1092	the predicted depth-wise compositional changes from each model	1047:1108	Finally, the predicted depth-wise compositional changes from each model were compared against experimentally observed compositional changes in vitro.
29150953	4	3	from	decrease	760:767	arg1	content					785:791	proteoglycan content	772:791	proteoglycan content	772:791	While excessive levels of tissue stresses controlled degeneration of the collagen fibril network, excessive levels of tissue strains controlled the decrease in proteoglycan content and the increase in permeability.
29150953	4	3	from	decrease	760:767	arg1	permeability					813:824	permeability	813:824	permeability	813:824	While excessive levels of tissue stresses controlled degeneration of the collagen fibril network, excessive levels of tissue strains controlled the decrease in proteoglycan content and the increase in permeability.
29150953	2	4	theme	knee	331:334	arg1	osteoarthritis					336:349	knee osteoarthritis	331:349	knee osteoarthritis	331:349	Currently, there is only one modeling framework which can be applied to predict the progression of knee osteoarthritis but it only considers degenerative changes in the collagen fibril network.
29150953	9	5	with	Consistent	1316:1325	arg1	earlier					1332:1338	earlier	1332:1338	earlier	1332:1338	Consistent with earlier in vitro experiments, this model simulated the greatest proteoglycan depletion in the superficial and middle zones, while the collagen fibril degeneration was located mostly in the superficial zone.
29150953	7	6	from	changes	1170:1176	arg1	vitro					1181:1185	vitro	1181:1185	vitro	1181:1185	Finally, the predicted depth-wise compositional changes from each model were compared against experimentally observed compositional changes in vitro.
29150953	10	7	theme	presented	1543:1551	arg1	algorithm					1553:1561	The presented algorithm	1539:1561	The presented algorithm	1539:1561	The presented algorithm can be used for predicting simultaneous collagen degeneration and proteoglycan loss during the development of knee osteoarthritis.
29150953	0	8	theme	knee	83:86	arg1	joint					88:92	the knee joint	79:92	the knee joint	79:92	New algorithm for simulation of proteoglycan loss and collagen degeneration in the knee joint: Data from the osteoarthritis initiative.
29150953	9	9	theme	vitro	1343:1347	arg1	experiments					1349:1359	vitro experiments	1343:1359	vitro experiments	1343:1359	Consistent with earlier in vitro experiments, this model simulated the greatest proteoglycan depletion in the superficial and middle zones, while the collagen fibril degeneration was located mostly in the superficial zone.
29150953	4	10	theme	strains	737:743	arg1	levels					720:725	excessive levels	710:725	excessive levels of tissue strains	710:743	While excessive levels of tissue stresses controlled degeneration of the collagen fibril network, excessive levels of tissue strains controlled the decrease in proteoglycan content and the increase in permeability.
29150953	10	11	theme	osteoarthritis	1678:1691	arg1	development					1658:1668	the development	1654:1668	the development of knee osteoarthritis	1654:1691	The presented algorithm can be used for predicting simultaneous collagen degeneration and proteoglycan loss during the development of knee osteoarthritis.
29150953	3	12	theme	proteoglycan	520:531	arg1	changes					511:517	the major tissue changes	494:517	the major tissue changes (proteoglycan content, fluid flow, and collagen fibril network) occurring in osteoarthritis	494:609	Here, we have developed the framework further by considering all of the major tissue changes (proteoglycan content, fluid flow, and collagen fibril network) occurring in osteoarthritis.
29150953	3	12	theme	proteoglycan	520:531	arg1	content					533:539	proteoglycan content	520:539	proteoglycan content	520:539	Here, we have developed the framework further by considering all of the major tissue changes (proteoglycan content, fluid flow, and collagen fibril network) occurring in osteoarthritis.
29150953	5	13	theme	depth-wise	912:921	arg1	composition					923:933	the depth-wise composition	908:933	the depth-wise composition	908:933	We created four knee joint models with increasing degrees of complexity based on the depth-wise composition.
29150953	9	14	theme	middle	1442:1447	arg1	zones					1449:1453	the superficial and middle zones	1422:1453	the superficial and middle zones	1422:1453	Consistent with earlier in vitro experiments, this model simulated the greatest proteoglycan depletion in the superficial and middle zones, while the collagen fibril degeneration was located mostly in the superficial zone.
29150953	5	15	theme	joint	848:852	arg1	models					854:859	four knee joint models	838:859	four knee joint models	838:859	We created four knee joint models with increasing degrees of complexity based on the depth-wise composition.
29150953	2	16	theme	modeling	261:268	arg1	framework					270:278	only one modeling framework	252:278	only one modeling framework which can be applied to predict the progression of knee osteoarthritis	252:349	Currently, there is only one modeling framework which can be applied to predict the progression of knee osteoarthritis but it only considers degenerative changes in the collagen fibril network.
29150953	1	17	theme	harmful	156:162	arg1	disease					170:176	a harmful joint disease	154:176	a harmful joint disease	154:176	Osteoarthritis is a harmful joint disease but prediction of disease progression is problematic.
29150953	1	17	theme	harmful	156:162	arg1	Osteoarthritis					136:149	Osteoarthritis	136:149	Osteoarthritis	136:149	Osteoarthritis is a harmful joint disease but prediction of disease progression is problematic.
29150953	6	18	theme	abnormal	970:977	arg1	conditions					1014:1023	loading conditions	1006:1023	loading conditions	1006:1023	Models were tested for normal and abnormal, physiologically relevant, loading conditions in the knee.
29150953	7	19	theme	compositional	1156:1168	arg1	changes					1170:1176	experimentally observed compositional changes	1132:1176	experimentally observed compositional changes in vitro	1132:1185	Finally, the predicted depth-wise compositional changes from each model were compared against experimentally observed compositional changes in vitro.
29150953	1	20	theme	joint	164:168	arg1	disease					170:176	a harmful joint disease	154:176	a harmful joint disease	154:176	Osteoarthritis is a harmful joint disease but prediction of disease progression is problematic.
29150953	1	20	theme	joint	164:168	arg1	Osteoarthritis					136:149	Osteoarthritis	136:149	Osteoarthritis	136:149	Osteoarthritis is a harmful joint disease but prediction of disease progression is problematic.
29150953	9	21	theme	greatest	1387:1394	arg1	depletion					1409:1417	the greatest proteoglycan depletion	1383:1417	the greatest proteoglycan depletion	1383:1417	Consistent with earlier in vitro experiments, this model simulated the greatest proteoglycan depletion in the superficial and middle zones, while the collagen fibril degeneration was located mostly in the superficial zone.
29150953	7	22	theme	compositional	1072:1084	arg1	changes					1086:1092	the predicted depth-wise compositional changes	1047:1092	the predicted depth-wise compositional changes from each model	1047:1108	Finally, the predicted depth-wise compositional changes from each model were compared against experimentally observed compositional changes in vitro.
29150953	10	23	theme	collagen	1603:1610	arg1	degeneration					1612:1623	simultaneous collagen degeneration	1590:1623	simultaneous collagen degeneration	1590:1623	The presented algorithm can be used for predicting simultaneous collagen degeneration and proteoglycan loss during the development of knee osteoarthritis.
29150953	3	24	theme	fluid	542:546	arg1	changes					511:517	the major tissue changes	494:517	the major tissue changes (proteoglycan content, fluid flow, and collagen fibril network) occurring in osteoarthritis	494:609	Here, we have developed the framework further by considering all of the major tissue changes (proteoglycan content, fluid flow, and collagen fibril network) occurring in osteoarthritis.
29150953	3	24	theme	fluid	542:546	arg1	flow					548:551	fluid flow	542:551	fluid flow	542:551	Here, we have developed the framework further by considering all of the major tissue changes (proteoglycan content, fluid flow, and collagen fibril network) occurring in osteoarthritis.
29150953	4	25	theme	stresses	645:652	arg1	levels					628:633	excessive levels	618:633	excessive levels of tissue stresses	618:652	While excessive levels of tissue stresses controlled degeneration of the collagen fibril network, excessive levels of tissue strains controlled the decrease in proteoglycan content and the increase in permeability.
29150953	0	26	theme	New	0:2	arg1	algorithm					4:12	New algorithm	0:12	New algorithm for simulation of proteoglycan loss and collagen degeneration in the knee joint: Data from the osteoarthritis initiative.	0:134	New algorithm for simulation of proteoglycan loss and collagen degeneration in the knee joint: Data from the osteoarthritis initiative.
29150953	10	27	theme	knee	1673:1676	arg1	osteoarthritis					1678:1691	knee osteoarthritis	1673:1691	knee osteoarthritis	1673:1691	The presented algorithm can be used for predicting simultaneous collagen degeneration and proteoglycan loss during the development of knee osteoarthritis.
29150953	9	28	from	simulated	1373:1381	arg1	zones					1449:1453	the superficial and middle zones	1422:1453	the superficial and middle zones	1422:1453	Consistent with earlier in vitro experiments, this model simulated the greatest proteoglycan depletion in the superficial and middle zones, while the collagen fibril degeneration was located mostly in the superficial zone.
29150953	9	29	from	zone	1533:1536	arg1	located					1499:1505	located	1499:1505	located	1499:1505	Consistent with earlier in vitro experiments, this model simulated the greatest proteoglycan depletion in the superficial and middle zones, while the collagen fibril degeneration was located mostly in the superficial zone.
29150953	4	30	theme	excessive	710:718	arg1	levels					720:725	excessive levels	710:725	excessive levels of tissue strains	710:743	While excessive levels of tissue stresses controlled degeneration of the collagen fibril network, excessive levels of tissue strains controlled the decrease in proteoglycan content and the increase in permeability.
29150953	4	31	theme	tissue	638:643	arg1	stresses					645:652	tissue stresses	638:652	tissue stresses	638:652	While excessive levels of tissue stresses controlled degeneration of the collagen fibril network, excessive levels of tissue strains controlled the decrease in proteoglycan content and the increase in permeability.
29150953	8	32	theme	typical	1216:1222	arg1	composition					1235:1245	the typical depth-wise composition	1212:1245	the typical depth-wise composition of cartilage	1212:1258	The model incorporating the typical depth-wise composition of cartilage produced the best match with experimental observations.
29150953	6	33	theme	normal	959:964	arg1	conditions					1014:1023	loading conditions	1006:1023	loading conditions	1006:1023	Models were tested for normal and abnormal, physiologically relevant, loading conditions in the knee.
29150953	7	34	theme	predicted	1051:1059	arg1	changes					1086:1092	the predicted depth-wise compositional changes	1047:1092	the predicted depth-wise compositional changes from each model	1047:1108	Finally, the predicted depth-wise compositional changes from each model were compared against experimentally observed compositional changes in vitro.
29150953	9	35	theme	fibril	1475:1480	arg1	degeneration					1482:1493	the collagen fibril degeneration	1462:1493	the collagen fibril degeneration	1462:1493	Consistent with earlier in vitro experiments, this model simulated the greatest proteoglycan depletion in the superficial and middle zones, while the collagen fibril degeneration was located mostly in the superficial zone.
29150953	4	36	theme	tissue	730:735	arg1	strains					737:743	tissue strains	730:743	tissue strains	730:743	While excessive levels of tissue stresses controlled degeneration of the collagen fibril network, excessive levels of tissue strains controlled the decrease in proteoglycan content and the increase in permeability.
29150953	10	37	used	used	1570:1573	arg2	algorithm					1553:1561	The presented algorithm	1539:1561	The presented algorithm	1539:1561	The presented algorithm can be used for predicting simultaneous collagen degeneration and proteoglycan loss during the development of knee osteoarthritis.
29150953	2	38	theme	fibril	410:415	arg1	network					417:423	the collagen fibril network	397:423	the collagen fibril network	397:423	Currently, there is only one modeling framework which can be applied to predict the progression of knee osteoarthritis but it only considers degenerative changes in the collagen fibril network.
29150953	8	39	dep	best	1273:1276	arg1	match					1278:1282	match	1278:1282	match	1278:1282	The model incorporating the typical depth-wise composition of cartilage produced the best match with experimental observations.
29150953	3	40	theme	tissue	504:509	arg1	network					574:580	collagen fibril network	558:580	collagen fibril network	558:580	Here, we have developed the framework further by considering all of the major tissue changes (proteoglycan content, fluid flow, and collagen fibril network) occurring in osteoarthritis.
29150953	3	40	theme	tissue	504:509	arg1	changes					511:517	the major tissue changes	494:517	the major tissue changes (proteoglycan content, fluid flow, and collagen fibril network) occurring in osteoarthritis	494:609	Here, we have developed the framework further by considering all of the major tissue changes (proteoglycan content, fluid flow, and collagen fibril network) occurring in osteoarthritis.
29150953	3	40	theme	tissue	504:509	arg1	content					533:539	proteoglycan content	520:539	proteoglycan content	520:539	Here, we have developed the framework further by considering all of the major tissue changes (proteoglycan content, fluid flow, and collagen fibril network) occurring in osteoarthritis.
29150953	3	40	theme	tissue	504:509	arg1	flow					548:551	fluid flow	542:551	fluid flow	542:551	Here, we have developed the framework further by considering all of the major tissue changes (proteoglycan content, fluid flow, and collagen fibril network) occurring in osteoarthritis.
29150953	5	41	theme	complexity	888:897	arg1	degrees					877:883	increasing degrees	866:883	increasing degrees of complexity	866:897	We created four knee joint models with increasing degrees of complexity based on the depth-wise composition.
29150953	9	42	theme	collagen	1466:1473	arg1	degeneration					1482:1493	the collagen fibril degeneration	1462:1493	the collagen fibril degeneration	1462:1493	Consistent with earlier in vitro experiments, this model simulated the greatest proteoglycan depletion in the superficial and middle zones, while the collagen fibril degeneration was located mostly in the superficial zone.
29150953	2	43	theme	collagen	401:408	arg1	network					417:423	the collagen fibril network	397:423	the collagen fibril network	397:423	Currently, there is only one modeling framework which can be applied to predict the progression of knee osteoarthritis but it only considers degenerative changes in the collagen fibril network.
29150953	0	44	theme	proteoglycan	32:43	arg1	loss					45:48	proteoglycan loss	32:48	proteoglycan loss	32:48	New algorithm for simulation of proteoglycan loss and collagen degeneration in the knee joint: Data from the osteoarthritis initiative.
29150953	4	45	theme	collagen	685:692	arg1	network					701:707	the collagen fibril network	681:707	the collagen fibril network	681:707	While excessive levels of tissue stresses controlled degeneration of the collagen fibril network, excessive levels of tissue strains controlled the decrease in proteoglycan content and the increase in permeability.
29150953	4	46	theme	excessive	618:626	arg1	levels					628:633	excessive levels	618:633	excessive levels of tissue stresses	618:652	While excessive levels of tissue stresses controlled degeneration of the collagen fibril network, excessive levels of tissue strains controlled the decrease in proteoglycan content and the increase in permeability.
29150953	3	47	theme	collagen	558:565	arg1	changes					511:517	the major tissue changes	494:517	the major tissue changes (proteoglycan content, fluid flow, and collagen fibril network) occurring in osteoarthritis	494:609	Here, we have developed the framework further by considering all of the major tissue changes (proteoglycan content, fluid flow, and collagen fibril network) occurring in osteoarthritis.
29150953	3	47	theme	collagen	558:565	arg1	network					574:580	collagen fibril network	558:580	collagen fibril network	558:580	Here, we have developed the framework further by considering all of the major tissue changes (proteoglycan content, fluid flow, and collagen fibril network) occurring in osteoarthritis.
29150953	1	48	theme	disease	196:202	arg1	progression					204:214	disease progression	196:214	disease progression	196:214	Osteoarthritis is a harmful joint disease but prediction of disease progression is problematic.
29150953	5	49	theme	knee	843:846	arg1	models					854:859	four knee joint models	838:859	four knee joint models	838:859	We created four knee joint models with increasing degrees of complexity based on the depth-wise composition.
29150953	7	50	from	model	1104:1108	arg1	changes					1086:1092	the predicted depth-wise compositional changes	1047:1092	the predicted depth-wise compositional changes from each model	1047:1108	Finally, the predicted depth-wise compositional changes from each model were compared against experimentally observed compositional changes in vitro.
29150953	0	51	theme	osteoarthritis	109:122	arg1	initiative					124:133	the osteoarthritis initiative	105:133	the osteoarthritis initiative	105:133	New algorithm for simulation of proteoglycan loss and collagen degeneration in the knee joint: Data from the osteoarthritis initiative.
29150953	11	52	theme	Orthopaedic	1701:1711	arg1	Research					1713:1720	© 2017 Orthopaedic Research	1694:1720	© 2017 Orthopaedic Research Society.	1694:1729	© 2017 Orthopaedic Research Society.
29150953	3	53	theme	fibril	567:572	arg1	changes					511:517	the major tissue changes	494:517	the major tissue changes (proteoglycan content, fluid flow, and collagen fibril network) occurring in osteoarthritis	494:609	Here, we have developed the framework further by considering all of the major tissue changes (proteoglycan content, fluid flow, and collagen fibril network) occurring in osteoarthritis.
29150953	3	53	theme	fibril	567:572	arg1	network					574:580	collagen fibril network	558:580	collagen fibril network	558:580	Here, we have developed the framework further by considering all of the major tissue changes (proteoglycan content, fluid flow, and collagen fibril network) occurring in osteoarthritis.
29150953	1	54	theme	progression	204:214	arg1	prediction					182:191	prediction	182:191	prediction of disease progression	182:214	Osteoarthritis is a harmful joint disease but prediction of disease progression is problematic.
29150953	8	55	theme	cartilage	1250:1258	arg1	composition					1235:1245	the typical depth-wise composition	1212:1245	the typical depth-wise composition of cartilage	1212:1258	The model incorporating the typical depth-wise composition of cartilage produced the best match with experimental observations.
29150953	9	56	theme	proteoglycan	1396:1407	arg1	depletion					1409:1417	the greatest proteoglycan depletion	1383:1417	the greatest proteoglycan depletion	1383:1417	Consistent with earlier in vitro experiments, this model simulated the greatest proteoglycan depletion in the superficial and middle zones, while the collagen fibril degeneration was located mostly in the superficial zone.
29150953	8	57	theme	depth-wise	1224:1233	arg1	composition					1235:1245	the typical depth-wise composition	1212:1245	the typical depth-wise composition of cartilage	1212:1258	The model incorporating the typical depth-wise composition of cartilage produced the best match with experimental observations.
29150953	10	58	theme	simultaneous	1590:1601	arg1	degeneration					1612:1623	simultaneous collagen degeneration	1590:1623	simultaneous collagen degeneration	1590:1623	The presented algorithm can be used for predicting simultaneous collagen degeneration and proteoglycan loss during the development of knee osteoarthritis.
29150953	4	59	theme	fibril	694:699	arg1	network					701:707	the collagen fibril network	681:707	the collagen fibril network	681:707	While excessive levels of tissue stresses controlled degeneration of the collagen fibril network, excessive levels of tissue strains controlled the decrease in proteoglycan content and the increase in permeability.
29150953	0	60	theme	loss	45:48	arg1	simulation					18:27	simulation	18:27	simulation of proteoglycan loss and collagen degeneration in the knee joint	18:92	New algorithm for simulation of proteoglycan loss and collagen degeneration in the knee joint: Data from the osteoarthritis initiative.
29150953	4	61	from	increase	801:808	arg1	content					785:791	proteoglycan content	772:791	proteoglycan content	772:791	While excessive levels of tissue stresses controlled degeneration of the collagen fibril network, excessive levels of tissue strains controlled the decrease in proteoglycan content and the increase in permeability.
29150953	4	61	from	increase	801:808	arg1	permeability					813:824	permeability	813:824	permeability	813:824	While excessive levels of tissue stresses controlled degeneration of the collagen fibril network, excessive levels of tissue strains controlled the decrease in proteoglycan content and the increase in permeability.
29150953	9	62	from	zones	1449:1453	arg1	simulated					1373:1381	simulated	1373:1381	simulated	1373:1381	Consistent with earlier in vitro experiments, this model simulated the greatest proteoglycan depletion in the superficial and middle zones, while the collagen fibril degeneration was located mostly in the superficial zone.
29150953	2	63	from	changes	386:392	arg1	network					417:423	the collagen fibril network	397:423	the collagen fibril network	397:423	Currently, there is only one modeling framework which can be applied to predict the progression of knee osteoarthritis but it only considers degenerative changes in the collagen fibril network.
29150953	3	64	dep	changes	511:517	arg1	changes					511:517	the major tissue changes	494:517	the major tissue changes (proteoglycan content, fluid flow, and collagen fibril network) occurring in osteoarthritis	494:609	Here, we have developed the framework further by considering all of the major tissue changes (proteoglycan content, fluid flow, and collagen fibril network) occurring in osteoarthritis.
29150953	3	64	dep	changes	511:517	arg1	network					574:580	collagen fibril network	558:580	collagen fibril network	558:580	Here, we have developed the framework further by considering all of the major tissue changes (proteoglycan content, fluid flow, and collagen fibril network) occurring in osteoarthritis.
29150953	3	64	dep	changes	511:517	arg1	content					533:539	proteoglycan content	520:539	proteoglycan content	520:539	Here, we have developed the framework further by considering all of the major tissue changes (proteoglycan content, fluid flow, and collagen fibril network) occurring in osteoarthritis.
29150953	3	64	dep	changes	511:517	arg1	flow					548:551	fluid flow	542:551	fluid flow	542:551	Here, we have developed the framework further by considering all of the major tissue changes (proteoglycan content, fluid flow, and collagen fibril network) occurring in osteoarthritis.
29150953	11	65	theme	©	1694:1694	arg1	Research					1713:1720	© 2017 Orthopaedic Research	1694:1720	© 2017 Orthopaedic Research Society.	1694:1729	© 2017 Orthopaedic Research Society.
29150953	7	66	theme	observed	1147:1154	arg1	changes					1170:1176	experimentally observed compositional changes	1132:1176	experimentally observed compositional changes in vitro	1132:1185	Finally, the predicted depth-wise compositional changes from each model were compared against experimentally observed compositional changes in vitro.
29150953	4	67	theme	proteoglycan	772:783	arg1	content					785:791	proteoglycan content	772:791	proteoglycan content	772:791	While excessive levels of tissue stresses controlled degeneration of the collagen fibril network, excessive levels of tissue strains controlled the decrease in proteoglycan content and the increase in permeability.
29150953	9	68	from	located	1499:1505	arg1	zone					1533:1536	the superficial zone	1517:1536	the superficial zone	1517:1536	Consistent with earlier in vitro experiments, this model simulated the greatest proteoglycan depletion in the superficial and middle zones, while the collagen fibril degeneration was located mostly in the superficial zone.
29150953	9	69	theme	superficial	1426:1436	arg1	zones					1449:1453	the superficial and middle zones	1422:1453	the superficial and middle zones	1422:1453	Consistent with earlier in vitro experiments, this model simulated the greatest proteoglycan depletion in the superficial and middle zones, while the collagen fibril degeneration was located mostly in the superficial zone.
29150953	0	70	from	initiative	124:133	arg1	Data					95:98	Data	95:98	New algorithm for simulation of proteoglycan loss and collagen degeneration in the knee joint: Data from the osteoarthritis initiative.	0:134	New algorithm for simulation of proteoglycan loss and collagen degeneration in the knee joint: Data from the osteoarthritis initiative.
29150953	4	71	theme	network	701:707	arg1	degeneration					665:676	degeneration	665:676	degeneration of the collagen fibril network	665:707	While excessive levels of tissue stresses controlled degeneration of the collagen fibril network, excessive levels of tissue strains controlled the decrease in proteoglycan content and the increase in permeability.
29150953	0	72	theme	degeneration	63:74	arg1	simulation					18:27	simulation	18:27	simulation of proteoglycan loss and collagen degeneration in the knee joint	18:92	New algorithm for simulation of proteoglycan loss and collagen degeneration in the knee joint: Data from the osteoarthritis initiative.
29150953	9	73	theme	superficial	1521:1531	arg1	zone					1533:1536	the superficial zone	1517:1536	the superficial zone	1517:1536	Consistent with earlier in vitro experiments, this model simulated the greatest proteoglycan depletion in the superficial and middle zones, while the collagen fibril degeneration was located mostly in the superficial zone.
29150953	0	74	dep	algorithm	4:12	arg1	Data					95:98	Data	95:98	New algorithm for simulation of proteoglycan loss and collagen degeneration in the knee joint: Data from the osteoarthritis initiative.	0:134	New algorithm for simulation of proteoglycan loss and collagen degeneration in the knee joint: Data from the osteoarthritis initiative.
29150953	8	75	theme	experimental	1289:1300	arg1	observations					1302:1313	experimental observations	1289:1313	experimental observations	1289:1313	The model incorporating the typical depth-wise composition of cartilage produced the best match with experimental observations.
29150953	5	76	theme	increasing	866:875	arg1	degrees					877:883	increasing degrees	866:883	increasing degrees of complexity	866:897	We created four knee joint models with increasing degrees of complexity based on the depth-wise composition.
29150953	2	77	theme	degenerative	373:384	arg1	changes					386:392	degenerative changes	373:392	degenerative changes in the collagen fibril network	373:423	Currently, there is only one modeling framework which can be applied to predict the progression of knee osteoarthritis but it only considers degenerative changes in the collagen fibril network.
29150953	6	78	theme	loading	1006:1012	arg1	conditions					1014:1023	loading conditions	1006:1023	loading conditions	1006:1023	Models were tested for normal and abnormal, physiologically relevant, loading conditions in the knee.
29150953	0	79	theme	collagen	54:61	arg1	degeneration					63:74	collagen degeneration	54:74	collagen degeneration	54:74	New algorithm for simulation of proteoglycan loss and collagen degeneration in the knee joint: Data from the osteoarthritis initiative.
29150953	0	80	from	simulation	18:27	arg1	joint					88:92	the knee joint	79:92	the knee joint	79:92	New algorithm for simulation of proteoglycan loss and collagen degeneration in the knee joint: Data from the osteoarthritis initiative.
29150953	6	81	from	conditions	1014:1023	arg1	knee					1032:1035	the knee	1028:1035	the knee	1028:1035	Models were tested for normal and abnormal, physiologically relevant, loading conditions in the knee.
29150953	3	82	theme	major	498:502	arg1	network					574:580	collagen fibril network	558:580	collagen fibril network	558:580	Here, we have developed the framework further by considering all of the major tissue changes (proteoglycan content, fluid flow, and collagen fibril network) occurring in osteoarthritis.
29150953	3	82	theme	major	498:502	arg1	changes					511:517	the major tissue changes	494:517	the major tissue changes (proteoglycan content, fluid flow, and collagen fibril network) occurring in osteoarthritis	494:609	Here, we have developed the framework further by considering all of the major tissue changes (proteoglycan content, fluid flow, and collagen fibril network) occurring in osteoarthritis.
29150953	3	82	theme	major	498:502	arg1	content					533:539	proteoglycan content	520:539	proteoglycan content	520:539	Here, we have developed the framework further by considering all of the major tissue changes (proteoglycan content, fluid flow, and collagen fibril network) occurring in osteoarthritis.
29150953	3	82	theme	major	498:502	arg1	flow					548:551	fluid flow	542:551	fluid flow	542:551	Here, we have developed the framework further by considering all of the major tissue changes (proteoglycan content, fluid flow, and collagen fibril network) occurring in osteoarthritis.
29150953	10	83	theme	proteoglycan	1629:1640	arg1	loss					1642:1645	proteoglycan loss	1629:1645	proteoglycan loss	1629:1645	The presented algorithm can be used for predicting simultaneous collagen degeneration and proteoglycan loss during the development of knee osteoarthritis.
28356486	1	0	dep	Brown	260:264	arg1	al.					269:271	Brown et al.	260:271	Brown et al.	260:271	In a recent publication in Bioscience Reports "Contaminants in commercial preparations of 'purified' small leucine-rich proteoglycans may distort mechanistic studies", Brown et al. identified by mass spectrometry and immunoblotting that certain commercial preparations of the small leucine-rich proteoglycans (SLRPs) decorin and biglycan, in fact, contained a mix of several proteoglycans that also included fibromodulin and aggrecan.
28356486	4	1	theme	tissue	831:836	arg1	materials					850:858	tissue engineering materials	831:858	tissue engineering materials	831:858	Decorin is of interest as a transforming growth factor-β antagonist and is also finding use in tissue engineering materials.
28356486	1	2	theme	proteoglycans	212:224	arg1	preparations					166:177	commercial preparations	155:177	commercial preparations of 'purified' small leucine-rich proteoglycans	155:224	In a recent publication in Bioscience Reports "Contaminants in commercial preparations of 'purified' small leucine-rich proteoglycans may distort mechanistic studies", Brown et al. identified by mass spectrometry and immunoblotting that certain commercial preparations of the small leucine-rich proteoglycans (SLRPs) decorin and biglycan, in fact, contained a mix of several proteoglycans that also included fibromodulin and aggrecan.
28356486	1	3	theme	Bioscience	119:128	arg1	Reports					130:136	Bioscience Reports	119:136	Bioscience Reports	119:136	In a recent publication in Bioscience Reports "Contaminants in commercial preparations of 'purified' small leucine-rich proteoglycans may distort mechanistic studies", Brown et al. identified by mass spectrometry and immunoblotting that certain commercial preparations of the small leucine-rich proteoglycans (SLRPs) decorin and biglycan, in fact, contained a mix of several proteoglycans that also included fibromodulin and aggrecan.
28356486	1	4	from	Contaminants	139:150	arg1	preparations					166:177	commercial preparations	155:177	commercial preparations of 'purified' small leucine-rich proteoglycans	155:224	In a recent publication in Bioscience Reports "Contaminants in commercial preparations of 'purified' small leucine-rich proteoglycans may distort mechanistic studies", Brown et al. identified by mass spectrometry and immunoblotting that certain commercial preparations of the small leucine-rich proteoglycans (SLRPs) decorin and biglycan, in fact, contained a mix of several proteoglycans that also included fibromodulin and aggrecan.
28356486	4	5	from	use	824:826	arg1	materials					850:858	tissue engineering materials	831:858	tissue engineering materials	831:858	Decorin is of interest as a transforming growth factor-β antagonist and is also finding use in tissue engineering materials.
28356486	4	6	theme	engineering	838:848	arg1	materials					850:858	tissue engineering materials	831:858	tissue engineering materials	831:858	Decorin is of interest as a transforming growth factor-β antagonist and is also finding use in tissue engineering materials.
28356486	2	7	theme	biglycan	610:617	arg1	activities					585:594	specific activities	576:594	specific activities of decorin or biglycan	576:617	The preparations were thus not suitable to study specific activities of decorin or biglycan.
28356486	1	8	theme	mechanistic	238:248	arg1	studies					250:256	mechanistic studies	238:256	mechanistic studies	238:256	In a recent publication in Bioscience Reports "Contaminants in commercial preparations of 'purified' small leucine-rich proteoglycans may distort mechanistic studies", Brown et al. identified by mass spectrometry and immunoblotting that certain commercial preparations of the small leucine-rich proteoglycans (SLRPs) decorin and biglycan, in fact, contained a mix of several proteoglycans that also included fibromodulin and aggrecan.
28356486	1	9	theme	certain	329:335	arg1	biglycan					421:428	biglycan	421:428	biglycan	421:428	In a recent publication in Bioscience Reports "Contaminants in commercial preparations of 'purified' small leucine-rich proteoglycans may distort mechanistic studies", Brown et al. identified by mass spectrometry and immunoblotting that certain commercial preparations of the small leucine-rich proteoglycans (SLRPs) decorin and biglycan, in fact, contained a mix of several proteoglycans that also included fibromodulin and aggrecan.
28356486	1	9	theme	certain	329:335	arg1	decorin					409:415	decorin	409:415	decorin	409:415	In a recent publication in Bioscience Reports "Contaminants in commercial preparations of 'purified' small leucine-rich proteoglycans may distort mechanistic studies", Brown et al. identified by mass spectrometry and immunoblotting that certain commercial preparations of the small leucine-rich proteoglycans (SLRPs) decorin and biglycan, in fact, contained a mix of several proteoglycans that also included fibromodulin and aggrecan.
28356486	1	9	theme	certain	329:335	arg1	preparations					348:359	certain commercial preparations	329:359	certain commercial preparations of the small leucine-rich proteoglycans (SLRPs) decorin and biglycan	329:428	In a recent publication in Bioscience Reports "Contaminants in commercial preparations of 'purified' small leucine-rich proteoglycans may distort mechanistic studies", Brown et al. identified by mass spectrometry and immunoblotting that certain commercial preparations of the small leucine-rich proteoglycans (SLRPs) decorin and biglycan, in fact, contained a mix of several proteoglycans that also included fibromodulin and aggrecan.
28356486	2	10	theme	decorin	599:605	arg1	activities					585:594	specific activities	576:594	specific activities of decorin or biglycan	576:617	The preparations were thus not suitable to study specific activities of decorin or biglycan.
28356486	1	11	from	publication	104:114	arg1	Reports					130:136	Bioscience Reports	119:136	Bioscience Reports	119:136	In a recent publication in Bioscience Reports "Contaminants in commercial preparations of 'purified' small leucine-rich proteoglycans may distort mechanistic studies", Brown et al. identified by mass spectrometry and immunoblotting that certain commercial preparations of the small leucine-rich proteoglycans (SLRPs) decorin and biglycan, in fact, contained a mix of several proteoglycans that also included fibromodulin and aggrecan.
28356486	5	12	dep	Brown	887:891	arg1	al.					896:898	Brown et al.	887:898	Brown et al.'s findings	887:909	This Commentary discusses Brown et al.'s findings and general issues raised for researchers who work with commercially sourced purified proteoglycans.
28356486	1	13	theme	commercial	337:346	arg1	biglycan					421:428	biglycan	421:428	biglycan	421:428	In a recent publication in Bioscience Reports "Contaminants in commercial preparations of 'purified' small leucine-rich proteoglycans may distort mechanistic studies", Brown et al. identified by mass spectrometry and immunoblotting that certain commercial preparations of the small leucine-rich proteoglycans (SLRPs) decorin and biglycan, in fact, contained a mix of several proteoglycans that also included fibromodulin and aggrecan.
28356486	1	13	theme	commercial	337:346	arg1	decorin					409:415	decorin	409:415	decorin	409:415	In a recent publication in Bioscience Reports "Contaminants in commercial preparations of 'purified' small leucine-rich proteoglycans may distort mechanistic studies", Brown et al. identified by mass spectrometry and immunoblotting that certain commercial preparations of the small leucine-rich proteoglycans (SLRPs) decorin and biglycan, in fact, contained a mix of several proteoglycans that also included fibromodulin and aggrecan.
28356486	1	13	theme	commercial	337:346	arg1	preparations					348:359	certain commercial preparations	329:359	certain commercial preparations of the small leucine-rich proteoglycans (SLRPs) decorin and biglycan	329:428	In a recent publication in Bioscience Reports "Contaminants in commercial preparations of 'purified' small leucine-rich proteoglycans may distort mechanistic studies", Brown et al. identified by mass spectrometry and immunoblotting that certain commercial preparations of the small leucine-rich proteoglycans (SLRPs) decorin and biglycan, in fact, contained a mix of several proteoglycans that also included fibromodulin and aggrecan.
28356486	0	14	dep	sweet	54:58	arg1	will					39:42	will	39:42	will	39:42	Caveat emptor: for researchers, a rose will not smell sweet unless we know its composition.
28356486	0	15	theme	Caveat	0:5	arg1	emptor					7:12	Caveat emptor	0:12	Caveat emptor: for researchers, a rose will not smell sweet unless we know its composition.	0:90	Caveat emptor: for researchers, a rose will not smell sweet unless we know its composition.
28356486	2	16	theme	specific	576:583	arg1	activities					585:594	specific activities	576:594	specific activities of decorin or biglycan	576:617	The preparations were thus not suitable to study specific activities of decorin or biglycan.
28356486	4	17	theme	transforming	764:775	arg1	antagonist					793:802	a transforming growth factor-β antagonist	762:802	a transforming growth factor-β antagonist	762:802	Decorin is of interest as a transforming growth factor-β antagonist and is also finding use in tissue engineering materials.
28356486	5	18	theme	general	915:921	arg1	issues					923:928	general issues	915:928	general issues raised for researchers who work with commercially sourced purified proteoglycans	915:1009	This Commentary discusses Brown et al.'s findings and general issues raised for researchers who work with commercially sourced purified proteoglycans.
28356486	5	19	theme	purified	988:995	arg1	proteoglycans					997:1009	commercially sourced purified proteoglycans	967:1009	commercially sourced purified proteoglycans	967:1009	This Commentary discusses Brown et al.'s findings and general issues raised for researchers who work with commercially sourced purified proteoglycans.
28356486	1	20	theme	commercial	155:164	arg1	preparations					166:177	commercial preparations	155:177	commercial preparations of 'purified' small leucine-rich proteoglycans	155:224	In a recent publication in Bioscience Reports "Contaminants in commercial preparations of 'purified' small leucine-rich proteoglycans may distort mechanistic studies", Brown et al. identified by mass spectrometry and immunoblotting that certain commercial preparations of the small leucine-rich proteoglycans (SLRPs) decorin and biglycan, in fact, contained a mix of several proteoglycans that also included fibromodulin and aggrecan.
28356486	3	21	theme	multi-functional	701:716	arg1	effects					718:724	highly multi-functional effects	694:724	highly multi-functional effects	694:724	Decorin and biglycan are widely studied SLRPs that are considered to have highly multi-functional effects on cells.
28356486	1	22	dep	publication	104:114	arg1	distort					230:236	distort	230:236	may distort mechanistic studies"	226:257	In a recent publication in Bioscience Reports "Contaminants in commercial preparations of 'purified' small leucine-rich proteoglycans may distort mechanistic studies", Brown et al. identified by mass spectrometry and immunoblotting that certain commercial preparations of the small leucine-rich proteoglycans (SLRPs) decorin and biglycan, in fact, contained a mix of several proteoglycans that also included fibromodulin and aggrecan.
28356486	1	23	contain	contained	440:448	arg1	decorin					409:415	decorin	409:415	decorin	409:415	In a recent publication in Bioscience Reports "Contaminants in commercial preparations of 'purified' small leucine-rich proteoglycans may distort mechanistic studies", Brown et al. identified by mass spectrometry and immunoblotting that certain commercial preparations of the small leucine-rich proteoglycans (SLRPs) decorin and biglycan, in fact, contained a mix of several proteoglycans that also included fibromodulin and aggrecan.
28356486	1	23	contain	contained	440:448	arg2	spectrometry					292:303	mass spectrometry	287:303	mass spectrometry	287:303	In a recent publication in Bioscience Reports "Contaminants in commercial preparations of 'purified' small leucine-rich proteoglycans may distort mechanistic studies", Brown et al. identified by mass spectrometry and immunoblotting that certain commercial preparations of the small leucine-rich proteoglycans (SLRPs) decorin and biglycan, in fact, contained a mix of several proteoglycans that also included fibromodulin and aggrecan.
28356486	1	23	contain	contained	440:448	arg1	biglycan					421:428	biglycan	421:428	biglycan	421:428	In a recent publication in Bioscience Reports "Contaminants in commercial preparations of 'purified' small leucine-rich proteoglycans may distort mechanistic studies", Brown et al. identified by mass spectrometry and immunoblotting that certain commercial preparations of the small leucine-rich proteoglycans (SLRPs) decorin and biglycan, in fact, contained a mix of several proteoglycans that also included fibromodulin and aggrecan.
28356486	1	23	contain	contained	440:448	arg2	immunoblotting					309:322	immunoblotting	309:322	immunoblotting	309:322	In a recent publication in Bioscience Reports "Contaminants in commercial preparations of 'purified' small leucine-rich proteoglycans may distort mechanistic studies", Brown et al. identified by mass spectrometry and immunoblotting that certain commercial preparations of the small leucine-rich proteoglycans (SLRPs) decorin and biglycan, in fact, contained a mix of several proteoglycans that also included fibromodulin and aggrecan.
28356486	1	23	contain	contained	440:448	arg1	preparations					348:359	certain commercial preparations	329:359	certain commercial preparations of the small leucine-rich proteoglycans (SLRPs) decorin and biglycan	329:428	In a recent publication in Bioscience Reports "Contaminants in commercial preparations of 'purified' small leucine-rich proteoglycans may distort mechanistic studies", Brown et al. identified by mass spectrometry and immunoblotting that certain commercial preparations of the small leucine-rich proteoglycans (SLRPs) decorin and biglycan, in fact, contained a mix of several proteoglycans that also included fibromodulin and aggrecan.
28356486	1	23	contain	contained	440:448	arg2	decorin					409:415	decorin	409:415	decorin	409:415	In a recent publication in Bioscience Reports "Contaminants in commercial preparations of 'purified' small leucine-rich proteoglycans may distort mechanistic studies", Brown et al. identified by mass spectrometry and immunoblotting that certain commercial preparations of the small leucine-rich proteoglycans (SLRPs) decorin and biglycan, in fact, contained a mix of several proteoglycans that also included fibromodulin and aggrecan.
28356486	1	23	contain	contained	440:448	arg2	preparations					348:359	certain commercial preparations	329:359	certain commercial preparations of the small leucine-rich proteoglycans (SLRPs) decorin and biglycan	329:428	In a recent publication in Bioscience Reports "Contaminants in commercial preparations of 'purified' small leucine-rich proteoglycans may distort mechanistic studies", Brown et al. identified by mass spectrometry and immunoblotting that certain commercial preparations of the small leucine-rich proteoglycans (SLRPs) decorin and biglycan, in fact, contained a mix of several proteoglycans that also included fibromodulin and aggrecan.
28356486	1	23	contain	contained	440:448	arg1	fact					434:437	fact	434:437	fact	434:437	In a recent publication in Bioscience Reports "Contaminants in commercial preparations of 'purified' small leucine-rich proteoglycans may distort mechanistic studies", Brown et al. identified by mass spectrometry and immunoblotting that certain commercial preparations of the small leucine-rich proteoglycans (SLRPs) decorin and biglycan, in fact, contained a mix of several proteoglycans that also included fibromodulin and aggrecan.
28356486	1	23	contain	contained	440:448	arg2	mix					452:454	a mix	450:454	a mix of several proteoglycans that also included fibromodulin and aggrecan	450:524	In a recent publication in Bioscience Reports "Contaminants in commercial preparations of 'purified' small leucine-rich proteoglycans may distort mechanistic studies", Brown et al. identified by mass spectrometry and immunoblotting that certain commercial preparations of the small leucine-rich proteoglycans (SLRPs) decorin and biglycan, in fact, contained a mix of several proteoglycans that also included fibromodulin and aggrecan.
28356486	1	23	contain	contained	440:448	arg2	biglycan					421:428	biglycan	421:428	biglycan	421:428	In a recent publication in Bioscience Reports "Contaminants in commercial preparations of 'purified' small leucine-rich proteoglycans may distort mechanistic studies", Brown et al. identified by mass spectrometry and immunoblotting that certain commercial preparations of the small leucine-rich proteoglycans (SLRPs) decorin and biglycan, in fact, contained a mix of several proteoglycans that also included fibromodulin and aggrecan.
28356486	4	24	theme	factor-β	784:791	arg1	antagonist					793:802	a transforming growth factor-β antagonist	762:802	a transforming growth factor-β antagonist	762:802	Decorin is of interest as a transforming growth factor-β antagonist and is also finding use in tissue engineering materials.
28356486	1	25	theme	small	368:372	arg1	SLRPs					402:406	SLRPs	402:406	SLRPs	402:406	In a recent publication in Bioscience Reports "Contaminants in commercial preparations of 'purified' small leucine-rich proteoglycans may distort mechanistic studies", Brown et al. identified by mass spectrometry and immunoblotting that certain commercial preparations of the small leucine-rich proteoglycans (SLRPs) decorin and biglycan, in fact, contained a mix of several proteoglycans that also included fibromodulin and aggrecan.
28356486	1	25	theme	small	368:372	arg1	proteoglycans					387:399	the small leucine-rich proteoglycans	364:399	the small leucine-rich proteoglycans (SLRPs)	364:407	In a recent publication in Bioscience Reports "Contaminants in commercial preparations of 'purified' small leucine-rich proteoglycans may distort mechanistic studies", Brown et al. identified by mass spectrometry and immunoblotting that certain commercial preparations of the small leucine-rich proteoglycans (SLRPs) decorin and biglycan, in fact, contained a mix of several proteoglycans that also included fibromodulin and aggrecan.
28356486	4	26	theme	growth	777:782	arg1	antagonist					793:802	a transforming growth factor-β antagonist	762:802	a transforming growth factor-β antagonist	762:802	Decorin is of interest as a transforming growth factor-β antagonist and is also finding use in tissue engineering materials.
28356486	1	27	dep	preparations	348:359	arg1	biglycan					421:428	biglycan	421:428	biglycan	421:428	In a recent publication in Bioscience Reports "Contaminants in commercial preparations of 'purified' small leucine-rich proteoglycans may distort mechanistic studies", Brown et al. identified by mass spectrometry and immunoblotting that certain commercial preparations of the small leucine-rich proteoglycans (SLRPs) decorin and biglycan, in fact, contained a mix of several proteoglycans that also included fibromodulin and aggrecan.
28356486	1	27	dep	preparations	348:359	arg1	decorin					409:415	decorin	409:415	decorin	409:415	In a recent publication in Bioscience Reports "Contaminants in commercial preparations of 'purified' small leucine-rich proteoglycans may distort mechanistic studies", Brown et al. identified by mass spectrometry and immunoblotting that certain commercial preparations of the small leucine-rich proteoglycans (SLRPs) decorin and biglycan, in fact, contained a mix of several proteoglycans that also included fibromodulin and aggrecan.
28356486	1	27	dep	preparations	348:359	arg1	preparations					348:359	certain commercial preparations	329:359	certain commercial preparations of the small leucine-rich proteoglycans (SLRPs) decorin and biglycan	329:428	In a recent publication in Bioscience Reports "Contaminants in commercial preparations of 'purified' small leucine-rich proteoglycans may distort mechanistic studies", Brown et al. identified by mass spectrometry and immunoblotting that certain commercial preparations of the small leucine-rich proteoglycans (SLRPs) decorin and biglycan, in fact, contained a mix of several proteoglycans that also included fibromodulin and aggrecan.
28356486	1	28	theme	leucine-rich	374:385	arg1	SLRPs					402:406	SLRPs	402:406	SLRPs	402:406	In a recent publication in Bioscience Reports "Contaminants in commercial preparations of 'purified' small leucine-rich proteoglycans may distort mechanistic studies", Brown et al. identified by mass spectrometry and immunoblotting that certain commercial preparations of the small leucine-rich proteoglycans (SLRPs) decorin and biglycan, in fact, contained a mix of several proteoglycans that also included fibromodulin and aggrecan.
28356486	1	28	theme	leucine-rich	374:385	arg1	proteoglycans					387:399	the small leucine-rich proteoglycans	364:399	the small leucine-rich proteoglycans (SLRPs)	364:407	In a recent publication in Bioscience Reports "Contaminants in commercial preparations of 'purified' small leucine-rich proteoglycans may distort mechanistic studies", Brown et al. identified by mass spectrometry and immunoblotting that certain commercial preparations of the small leucine-rich proteoglycans (SLRPs) decorin and biglycan, in fact, contained a mix of several proteoglycans that also included fibromodulin and aggrecan.
28356486	1	29	theme	proteoglycans	387:399	arg1	biglycan					421:428	biglycan	421:428	biglycan	421:428	In a recent publication in Bioscience Reports "Contaminants in commercial preparations of 'purified' small leucine-rich proteoglycans may distort mechanistic studies", Brown et al. identified by mass spectrometry and immunoblotting that certain commercial preparations of the small leucine-rich proteoglycans (SLRPs) decorin and biglycan, in fact, contained a mix of several proteoglycans that also included fibromodulin and aggrecan.
28356486	1	29	theme	proteoglycans	387:399	arg1	decorin					409:415	decorin	409:415	decorin	409:415	In a recent publication in Bioscience Reports "Contaminants in commercial preparations of 'purified' small leucine-rich proteoglycans may distort mechanistic studies", Brown et al. identified by mass spectrometry and immunoblotting that certain commercial preparations of the small leucine-rich proteoglycans (SLRPs) decorin and biglycan, in fact, contained a mix of several proteoglycans that also included fibromodulin and aggrecan.
28356486	1	29	theme	proteoglycans	387:399	arg1	preparations					348:359	certain commercial preparations	329:359	certain commercial preparations of the small leucine-rich proteoglycans (SLRPs) decorin and biglycan	329:428	In a recent publication in Bioscience Reports "Contaminants in commercial preparations of 'purified' small leucine-rich proteoglycans may distort mechanistic studies", Brown et al. identified by mass spectrometry and immunoblotting that certain commercial preparations of the small leucine-rich proteoglycans (SLRPs) decorin and biglycan, in fact, contained a mix of several proteoglycans that also included fibromodulin and aggrecan.
28356486	1	30	theme	several	459:465	arg1	proteoglycans					467:479	several proteoglycans	459:479	several proteoglycans that also included fibromodulin and aggrecan	459:524	In a recent publication in Bioscience Reports "Contaminants in commercial preparations of 'purified' small leucine-rich proteoglycans may distort mechanistic studies", Brown et al. identified by mass spectrometry and immunoblotting that certain commercial preparations of the small leucine-rich proteoglycans (SLRPs) decorin and biglycan, in fact, contained a mix of several proteoglycans that also included fibromodulin and aggrecan.
28356486	1	31	theme	purified	183:190	arg1	proteoglycans					212:224	' small leucine-rich proteoglycans	191:224	'purified' small leucine-rich proteoglycans	182:224	In a recent publication in Bioscience Reports "Contaminants in commercial preparations of 'purified' small leucine-rich proteoglycans may distort mechanistic studies", Brown et al. identified by mass spectrometry and immunoblotting that certain commercial preparations of the small leucine-rich proteoglycans (SLRPs) decorin and biglycan, in fact, contained a mix of several proteoglycans that also included fibromodulin and aggrecan.
28356486	1	32	theme	proteoglycans	467:479	arg1	mix					452:454	a mix	450:454	a mix of several proteoglycans that also included fibromodulin and aggrecan	450:524	In a recent publication in Bioscience Reports "Contaminants in commercial preparations of 'purified' small leucine-rich proteoglycans may distort mechanistic studies", Brown et al. identified by mass spectrometry and immunoblotting that certain commercial preparations of the small leucine-rich proteoglycans (SLRPs) decorin and biglycan, in fact, contained a mix of several proteoglycans that also included fibromodulin and aggrecan.
28356486	3	33	contain	have	689:692	arg1	SLRPs					660:664	SLRPs	660:664	SLRPs that are considered to have highly multi-functional effects on cells	660:733	Decorin and biglycan are widely studied SLRPs that are considered to have highly multi-functional effects on cells.
28356486	3	33	contain	have	689:692	arg2	effects					718:724	highly multi-functional effects	694:724	highly multi-functional effects	694:724	Decorin and biglycan are widely studied SLRPs that are considered to have highly multi-functional effects on cells.
28356486	0	34	dep	emptor	7:12	arg1	sweet					54:58	sweet	54:58	sweet	54:58	Caveat emptor: for researchers, a rose will not smell sweet unless we know its composition.
28356486	1	35	theme	recent	97:102	arg1	publication					104:114	a recent publication	95:114	a recent publication in Bioscience Reports "Contaminants in commercial preparations of 'purified' small leucine-rich proteoglycans may distort mechanistic studies"	95:257	In a recent publication in Bioscience Reports "Contaminants in commercial preparations of 'purified' small leucine-rich proteoglycans may distort mechanistic studies", Brown et al. identified by mass spectrometry and immunoblotting that certain commercial preparations of the small leucine-rich proteoglycans (SLRPs) decorin and biglycan, in fact, contained a mix of several proteoglycans that also included fibromodulin and aggrecan.
28356486	1	36	theme	small	193:197	arg1	proteoglycans					212:224	' small leucine-rich proteoglycans	191:224	'purified' small leucine-rich proteoglycans	182:224	In a recent publication in Bioscience Reports "Contaminants in commercial preparations of 'purified' small leucine-rich proteoglycans may distort mechanistic studies", Brown et al. identified by mass spectrometry and immunoblotting that certain commercial preparations of the small leucine-rich proteoglycans (SLRPs) decorin and biglycan, in fact, contained a mix of several proteoglycans that also included fibromodulin and aggrecan.
28356486	1	37	theme	mass	287:290	arg1	spectrometry					292:303	mass spectrometry	287:303	mass spectrometry	287:303	In a recent publication in Bioscience Reports "Contaminants in commercial preparations of 'purified' small leucine-rich proteoglycans may distort mechanistic studies", Brown et al. identified by mass spectrometry and immunoblotting that certain commercial preparations of the small leucine-rich proteoglycans (SLRPs) decorin and biglycan, in fact, contained a mix of several proteoglycans that also included fibromodulin and aggrecan.
28356486	5	38	theme	sourced	980:986	arg1	proteoglycans					997:1009	commercially sourced purified proteoglycans	967:1009	commercially sourced purified proteoglycans	967:1009	This Commentary discusses Brown et al.'s findings and general issues raised for researchers who work with commercially sourced purified proteoglycans.
28356486	1	39	theme	leucine-rich	199:210	arg1	proteoglycans					212:224	' small leucine-rich proteoglycans	191:224	'purified' small leucine-rich proteoglycans	182:224	In a recent publication in Bioscience Reports "Contaminants in commercial preparations of 'purified' small leucine-rich proteoglycans may distort mechanistic studies", Brown et al. identified by mass spectrometry and immunoblotting that certain commercial preparations of the small leucine-rich proteoglycans (SLRPs) decorin and biglycan, in fact, contained a mix of several proteoglycans that also included fibromodulin and aggrecan.
29253994	0	0	theme	Achatina	87:94	arg1	fulica					96:101	Achatina fulica	87:101	Achatina fulica	87:101	Structural analysis and biological activity of a highly regular glycosaminoglycan from Achatina fulica.
29253994	7	1	theme	study	1201:1205	arg1	worthy					1183:1188	worthy	1183:1188	worthy of further study	1183:1205	Anticoagulant activity assays indicated that AF-GAG exhibits no anticoagulant activities, but considering its structural characteristic, other bioactivities such as heparanase inhibition may be worthy of further study.
29253994	3	2	dep	Fourier	351:357	arg1	transform					359:367	transform	359:367	transform infrared spectroscopy, analysis of monosaccharide composition, and 1D/2D nuclear magnetic resonance spectroscopy	359:480	Its structure was analyzed and characterized by chemical and instrumental methods, such as Fourier transform infrared spectroscopy, analysis of monosaccharide composition, and 1D/2D nuclear magnetic resonance spectroscopy.
29253994	1	3	from	medicine	161:168	arg1	countries					178:186	many countries	173:186	many countries	173:186	Edible snails have been widely used as a health food and medicine in many countries.
29253994	1	4	theme	health	145:150	arg1	snails					111:116	Edible snails	104:116	Edible snails	104:116	Edible snails have been widely used as a health food and medicine in many countries.
29253994	1	4	theme	health	145:150	arg1	food					152:155	a health food	143:155	a health food	143:155	Edible snails have been widely used as a health food and medicine in many countries.
29253994	6	5	theme	repeating	857:865	arg1	disaccharide					867:878	a uniform repeating disaccharide	847:878	a uniform repeating disaccharide	847:878	Although its structure with a uniform repeating disaccharide is similar to those of heparin and heparan sulfate, this GAG is structurally highly regular and homogeneous.
29253994	3	6	theme	chemical	308:315	arg1	Fourier					351:357	Fourier	351:357	Fourier transform infrared spectroscopy, analysis of monosaccharide composition, and 1D/2D nuclear magnetic resonance spectroscopy	351:480	Its structure was analyzed and characterized by chemical and instrumental methods, such as Fourier transform infrared spectroscopy, analysis of monosaccharide composition, and 1D/2D nuclear magnetic resonance spectroscopy.
29253994	3	6	theme	chemical	308:315	arg1	methods					334:340	chemical and instrumental methods	308:340	chemical and instrumental methods	308:340	Its structure was analyzed and characterized by chemical and instrumental methods, such as Fourier transform infrared spectroscopy, analysis of monosaccharide composition, and 1D/2D nuclear magnetic resonance spectroscopy.
29253994	0	7	from	analysis	11:18	arg1	fulica					96:101	Achatina fulica	87:101	Achatina fulica	87:101	Structural analysis and biological activity of a highly regular glycosaminoglycan from Achatina fulica.
29253994	3	8	dep	transform	359:367	arg1	infrared					369:376	infrared	369:376	transform infrared spectroscopy, analysis of monosaccharide composition, and 1D/2D nuclear magnetic resonance spectroscopy	359:480	Its structure was analyzed and characterized by chemical and instrumental methods, such as Fourier transform infrared spectroscopy, analysis of monosaccharide composition, and 1D/2D nuclear magnetic resonance spectroscopy.
29253994	5	9	theme	Structural	649:658	arg1	analysis					660:667	Structural analysis	649:667	Structural analysis	649:667	Structural analysis clarified that the uronic acid unit in glycosaminoglycan (GAG) is the fully epimerized and the sequence of AF-GAG is →4)-α-GlcNAc (1→4)-α-IdoA2S (1→.
29253994	1	10	from	food	152:155	arg1	countries					178:186	many countries	173:186	many countries	173:186	Edible snails have been widely used as a health food and medicine in many countries.
29253994	7	11	theme	further	1193:1199	arg1	study					1201:1205	further study	1193:1205	further study	1193:1205	Anticoagulant activity assays indicated that AF-GAG exhibits no anticoagulant activities, but considering its structural characteristic, other bioactivities such as heparanase inhibition may be worthy of further study.
29253994	4	12	theme	Chemical	483:490	arg1	analysis					504:511	Chemical composition analysis	483:511	Chemical composition analysis	483:511	Chemical composition analysis indicated that AF-GAG is composed of iduronic acid (IdoA) and N-acetyl-glucosamine (GlcNAc) and its average molecular weight is 118kDa.
29253994	6	13	theme	uniform	849:855	arg1	disaccharide					867:878	a uniform repeating disaccharide	847:878	a uniform repeating disaccharide	847:878	Although its structure with a uniform repeating disaccharide is similar to those of heparin and heparan sulfate, this GAG is structurally highly regular and homogeneous.
29253994	6	14	with	structure	832:840	arg1	disaccharide					867:878	a uniform repeating disaccharide	847:878	a uniform repeating disaccharide	847:878	Although its structure with a uniform repeating disaccharide is similar to those of heparin and heparan sulfate, this GAG is structurally highly regular and homogeneous.
29253994	3	15	theme	1D/2D	436:440	arg1	resonance					459:467	1D/2D nuclear magnetic resonance	436:467	1D/2D nuclear magnetic resonance spectroscopy	436:480	Its structure was analyzed and characterized by chemical and instrumental methods, such as Fourier transform infrared spectroscopy, analysis of monosaccharide composition, and 1D/2D nuclear magnetic resonance spectroscopy.
29253994	0	16	from	activity	35:42	arg1	fulica					96:101	Achatina fulica	87:101	Achatina fulica	87:101	Structural analysis and biological activity of a highly regular glycosaminoglycan from Achatina fulica.
29253994	7	17	theme	anticoagulant	1053:1065	arg1	activities					1067:1076	no anticoagulant activities	1050:1076	no anticoagulant activities	1050:1076	Anticoagulant activity assays indicated that AF-GAG exhibits no anticoagulant activities, but considering its structural characteristic, other bioactivities such as heparanase inhibition may be worthy of further study.
29253994	1	18	used	used	135:138	arg2	snails					111:116	Edible snails	104:116	Edible snails	104:116	Edible snails have been widely used as a health food and medicine in many countries.
29253994	1	18	used	used	135:138	arg2	food					152:155	a health food	143:155	a health food	143:155	Edible snails have been widely used as a health food and medicine in many countries.
29253994	1	18	used	used	135:138	arg2	medicine					161:168	medicine	161:168	medicine	161:168	Edible snails have been widely used as a health food and medicine in many countries.
29253994	3	19	theme	composition	419:429	arg1	analysis					392:399	analysis	392:399	analysis of monosaccharide composition	392:429	Its structure was analyzed and characterized by chemical and instrumental methods, such as Fourier transform infrared spectroscopy, analysis of monosaccharide composition, and 1D/2D nuclear magnetic resonance spectroscopy.
29253994	3	19	theme	composition	419:429	arg1	spectroscopy					378:389	spectroscopy	378:389	spectroscopy	378:389	Its structure was analyzed and characterized by chemical and instrumental methods, such as Fourier transform infrared spectroscopy, analysis of monosaccharide composition, and 1D/2D nuclear magnetic resonance spectroscopy.
29253994	3	19	theme	composition	419:429	arg1	spectroscopy					469:480	1D/2D nuclear magnetic resonance spectroscopy	436:480	1D/2D nuclear magnetic resonance spectroscopy	436:480	Its structure was analyzed and characterized by chemical and instrumental methods, such as Fourier transform infrared spectroscopy, analysis of monosaccharide composition, and 1D/2D nuclear magnetic resonance spectroscopy.
29253994	0	20	theme	Structural	0:9	arg1	analysis					11:18	Structural analysis	0:18	Structural analysis	0:18	Structural analysis and biological activity of a highly regular glycosaminoglycan from Achatina fulica.
29253994	1	21	theme	many	173:176	arg1	countries					178:186	many countries	173:186	many countries	173:186	Edible snails have been widely used as a health food and medicine in many countries.
29253994	0	22	theme	biological	24:33	arg1	activity					35:42	biological activity	24:42	biological activity	24:42	Structural analysis and biological activity of a highly regular glycosaminoglycan from Achatina fulica.
29253994	7	23	theme	structural	1099:1108	arg1	characteristic					1110:1123	its structural characteristic	1095:1123	its structural characteristic	1095:1123	Anticoagulant activity assays indicated that AF-GAG exhibits no anticoagulant activities, but considering its structural characteristic, other bioactivities such as heparanase inhibition may be worthy of further study.
29253994	1	24	theme	Edible	104:109	arg1	food					152:155	a health food	143:155	a health food	143:155	Edible snails have been widely used as a health food and medicine in many countries.
29253994	1	24	theme	Edible	104:109	arg1	medicine					161:168	medicine	161:168	medicine	161:168	Edible snails have been widely used as a health food and medicine in many countries.
29253994	1	24	theme	Edible	104:109	arg1	snails					111:116	Edible snails	104:116	Edible snails	104:116	Edible snails have been widely used as a health food and medicine in many countries.
29253994	3	25	theme	resonance	459:467	arg1	spectroscopy					469:480	1D/2D nuclear magnetic resonance spectroscopy	436:480	1D/2D nuclear magnetic resonance spectroscopy	436:480	Its structure was analyzed and characterized by chemical and instrumental methods, such as Fourier transform infrared spectroscopy, analysis of monosaccharide composition, and 1D/2D nuclear magnetic resonance spectroscopy.
29253994	7	26	theme	heparanase	1154:1163	arg1	inhibition					1165:1174	heparanase inhibition	1154:1174	heparanase inhibition	1154:1174	Anticoagulant activity assays indicated that AF-GAG exhibits no anticoagulant activities, but considering its structural characteristic, other bioactivities such as heparanase inhibition may be worthy of further study.
29253994	6	27	theme	heparan	915:921	arg1	sulfate					923:929	heparan sulfate	915:929	heparan sulfate	915:929	Although its structure with a uniform repeating disaccharide is similar to those of heparin and heparan sulfate, this GAG is structurally highly regular and homogeneous.
29253994	3	28	theme	nuclear	442:448	arg1	resonance					459:467	1D/2D nuclear magnetic resonance	436:467	1D/2D nuclear magnetic resonance spectroscopy	436:480	Its structure was analyzed and characterized by chemical and instrumental methods, such as Fourier transform infrared spectroscopy, analysis of monosaccharide composition, and 1D/2D nuclear magnetic resonance spectroscopy.
29253994	3	29	theme	monosaccharide	404:417	arg1	composition					419:429	monosaccharide composition	404:429	monosaccharide composition	404:429	Its structure was analyzed and characterized by chemical and instrumental methods, such as Fourier transform infrared spectroscopy, analysis of monosaccharide composition, and 1D/2D nuclear magnetic resonance spectroscopy.
29253994	5	30	theme	AF-GAG	776:781	arg1	1→					815:816	1→	815:816	1→	815:816	Structural analysis clarified that the uronic acid unit in glycosaminoglycan (GAG) is the fully epimerized and the sequence of AF-GAG is →4)-α-GlcNAc (1→4)-α-IdoA2S (1→.
29253994	5	30	theme	AF-GAG	776:781	arg1	-α-IdoA2S					804:812	-α-IdoA2S	804:812	-α-IdoA2S	804:812	Structural analysis clarified that the uronic acid unit in glycosaminoglycan (GAG) is the fully epimerized and the sequence of AF-GAG is →4)-α-GlcNAc (1→4)-α-IdoA2S (1→.
29253994	5	30	theme	AF-GAG	776:781	arg1	sequence					764:771	the sequence	760:771	the sequence of AF-GAG	760:781	Structural analysis clarified that the uronic acid unit in glycosaminoglycan (GAG) is the fully epimerized and the sequence of AF-GAG is →4)-α-GlcNAc (1→4)-α-IdoA2S (1→.
29253994	3	31	theme	magnetic	450:457	arg1	resonance					459:467	1D/2D nuclear magnetic resonance	436:467	1D/2D nuclear magnetic resonance spectroscopy	436:480	Its structure was analyzed and characterized by chemical and instrumental methods, such as Fourier transform infrared spectroscopy, analysis of monosaccharide composition, and 1D/2D nuclear magnetic resonance spectroscopy.
29253994	4	32	theme	composition	492:502	arg1	analysis					504:511	Chemical composition analysis	483:511	Chemical composition analysis	483:511	Chemical composition analysis indicated that AF-GAG is composed of iduronic acid (IdoA) and N-acetyl-glucosamine (GlcNAc) and its average molecular weight is 118kDa.
29253994	3	33	theme	instrumental	321:332	arg1	Fourier					351:357	Fourier	351:357	Fourier transform infrared spectroscopy, analysis of monosaccharide composition, and 1D/2D nuclear magnetic resonance spectroscopy	351:480	Its structure was analyzed and characterized by chemical and instrumental methods, such as Fourier transform infrared spectroscopy, analysis of monosaccharide composition, and 1D/2D nuclear magnetic resonance spectroscopy.
29253994	3	33	theme	instrumental	321:332	arg1	methods					334:340	chemical and instrumental methods	308:340	chemical and instrumental methods	308:340	Its structure was analyzed and characterized by chemical and instrumental methods, such as Fourier transform infrared spectroscopy, analysis of monosaccharide composition, and 1D/2D nuclear magnetic resonance spectroscopy.
29253994	7	34	theme	Anticoagulant	989:1001	arg1	assays					1012:1017	Anticoagulant activity assays	989:1017	Anticoagulant activity assays	989:1017	Anticoagulant activity assays indicated that AF-GAG exhibits no anticoagulant activities, but considering its structural characteristic, other bioactivities such as heparanase inhibition may be worthy of further study.
29253994	7	35	theme	other	1126:1130	arg1	bioactivities					1132:1144	other bioactivities	1126:1144	other bioactivities such as heparanase inhibition	1126:1174	Anticoagulant activity assays indicated that AF-GAG exhibits no anticoagulant activities, but considering its structural characteristic, other bioactivities such as heparanase inhibition may be worthy of further study.
29253994	7	35	theme	other	1126:1130	arg1	inhibition					1165:1174	heparanase inhibition	1154:1174	heparanase inhibition	1154:1174	Anticoagulant activity assays indicated that AF-GAG exhibits no anticoagulant activities, but considering its structural characteristic, other bioactivities such as heparanase inhibition may be worthy of further study.
29253994	5	36	theme	uronic	688:693	arg1	unit					700:703	the uronic acid unit	684:703	the uronic acid unit in glycosaminoglycan (GAG)	684:730	Structural analysis clarified that the uronic acid unit in glycosaminoglycan (GAG) is the fully epimerized and the sequence of AF-GAG is →4)-α-GlcNAc (1→4)-α-IdoA2S (1→.
29253994	5	36	theme	uronic	688:693	arg1	epimerized					745:754	epimerized	745:754	epimerized	745:754	Structural analysis clarified that the uronic acid unit in glycosaminoglycan (GAG) is the fully epimerized and the sequence of AF-GAG is →4)-α-GlcNAc (1→4)-α-IdoA2S (1→.
29253994	2	37	theme	unique	191:196	arg1	AF-GAG					217:222	AF-GAG	217:222	AF-GAG	217:222	A unique glycosaminoglycan (AF-GAG) was purified from Achatina fulica.
29253994	2	37	theme	unique	191:196	arg1	glycosaminoglycan					198:214	A unique glycosaminoglycan	189:214	A unique glycosaminoglycan (AF-GAG)	189:223	A unique glycosaminoglycan (AF-GAG) was purified from Achatina fulica.
29253994	4	38	theme	iduronic	550:557	arg1	IdoA					565:568	IdoA	565:568	IdoA	565:568	Chemical composition analysis indicated that AF-GAG is composed of iduronic acid (IdoA) and N-acetyl-glucosamine (GlcNAc) and its average molecular weight is 118kDa.
29253994	4	38	theme	iduronic	550:557	arg1	acid					559:562	iduronic acid	550:562	iduronic acid (IdoA)	550:569	Chemical composition analysis indicated that AF-GAG is composed of iduronic acid (IdoA) and N-acetyl-glucosamine (GlcNAc) and its average molecular weight is 118kDa.
29253994	5	39	theme	acid	695:698	arg1	unit					700:703	the uronic acid unit	684:703	the uronic acid unit in glycosaminoglycan (GAG)	684:730	Structural analysis clarified that the uronic acid unit in glycosaminoglycan (GAG) is the fully epimerized and the sequence of AF-GAG is →4)-α-GlcNAc (1→4)-α-IdoA2S (1→.
29253994	5	39	theme	acid	695:698	arg1	epimerized					745:754	epimerized	745:754	epimerized	745:754	Structural analysis clarified that the uronic acid unit in glycosaminoglycan (GAG) is the fully epimerized and the sequence of AF-GAG is →4)-α-GlcNAc (1→4)-α-IdoA2S (1→.
29253994	2	40	theme	Achatina	243:250	arg1	fulica					252:257	Achatina fulica	243:257	Achatina fulica	243:257	A unique glycosaminoglycan (AF-GAG) was purified from Achatina fulica.
29253994	7	41	theme	activity	1003:1010	arg1	assays					1012:1017	Anticoagulant activity assays	989:1017	Anticoagulant activity assays	989:1017	Anticoagulant activity assays indicated that AF-GAG exhibits no anticoagulant activities, but considering its structural characteristic, other bioactivities such as heparanase inhibition may be worthy of further study.
29253994	5	42	from	unit	700:703	arg1	glycosaminoglycan					708:724	glycosaminoglycan	708:724	glycosaminoglycan (GAG)	708:730	Structural analysis clarified that the uronic acid unit in glycosaminoglycan (GAG) is the fully epimerized and the sequence of AF-GAG is →4)-α-GlcNAc (1→4)-α-IdoA2S (1→.
29253994	5	42	from	unit	700:703	arg1	GAG					727:729	GAG	727:729	GAG	727:729	Structural analysis clarified that the uronic acid unit in glycosaminoglycan (GAG) is the fully epimerized and the sequence of AF-GAG is →4)-α-GlcNAc (1→4)-α-IdoA2S (1→.
29253994	0	43	theme	glycosaminoglycan	64:80	arg1	analysis					11:18	Structural analysis	0:18	Structural analysis	0:18	Structural analysis and biological activity of a highly regular glycosaminoglycan from Achatina fulica.
29253994	0	43	theme	glycosaminoglycan	64:80	arg1	activity					35:42	biological activity	24:42	biological activity	24:42	Structural analysis and biological activity of a highly regular glycosaminoglycan from Achatina fulica.
29253994	0	44	from	fulica	96:101	arg1	analysis					11:18	Structural analysis	0:18	Structural analysis	0:18	Structural analysis and biological activity of a highly regular glycosaminoglycan from Achatina fulica.
29253994	0	44	from	fulica	96:101	arg1	activity					35:42	biological activity	24:42	biological activity	24:42	Structural analysis and biological activity of a highly regular glycosaminoglycan from Achatina fulica.
29253994	0	44	from	fulica	96:101	arg1	glycosaminoglycan					64:80	a highly regular glycosaminoglycan	47:80	a highly regular glycosaminoglycan from Achatina fulica	47:101	Structural analysis and biological activity of a highly regular glycosaminoglycan from Achatina fulica.
29253994	4	45	theme	molecular	621:629	arg1	weight					631:636	its average molecular weight	609:636	its average molecular weight	609:636	Chemical composition analysis indicated that AF-GAG is composed of iduronic acid (IdoA) and N-acetyl-glucosamine (GlcNAc) and its average molecular weight is 118kDa.
29253994	0	46	theme	regular	56:62	arg1	glycosaminoglycan					64:80	a highly regular glycosaminoglycan	47:80	a highly regular glycosaminoglycan from Achatina fulica	47:101	Structural analysis and biological activity of a highly regular glycosaminoglycan from Achatina fulica.
29253994	5	47	dep	-α-IdoA2S	804:812	arg1	1→					815:816	1→	815:816	1→	815:816	Structural analysis clarified that the uronic acid unit in glycosaminoglycan (GAG) is the fully epimerized and the sequence of AF-GAG is →4)-α-GlcNAc (1→4)-α-IdoA2S (1→.
29253994	5	47	dep	-α-IdoA2S	804:812	arg1	-α-IdoA2S					804:812	-α-IdoA2S	804:812	-α-IdoA2S	804:812	Structural analysis clarified that the uronic acid unit in glycosaminoglycan (GAG) is the fully epimerized and the sequence of AF-GAG is →4)-α-GlcNAc (1→4)-α-IdoA2S (1→.
29253994	5	47	dep	-α-IdoA2S	804:812	arg1	sequence					764:771	the sequence	760:771	the sequence of AF-GAG	760:781	Structural analysis clarified that the uronic acid unit in glycosaminoglycan (GAG) is the fully epimerized and the sequence of AF-GAG is →4)-α-GlcNAc (1→4)-α-IdoA2S (1→.
29253994	4	48	theme	average	613:619	arg1	weight					631:636	its average molecular weight	609:636	its average molecular weight	609:636	Chemical composition analysis indicated that AF-GAG is composed of iduronic acid (IdoA) and N-acetyl-glucosamine (GlcNAc) and its average molecular weight is 118kDa.
24188835	5	0	from	nanowhiskers	784:795	arg1	both					800:803	both	800:803	both	800:803	Stress-transfer mechanisms, between the matrix and the nanowhiskers in both of these nanocomposites are reported.
24188835	5	0	from	nanowhiskers	784:795	arg1	nanocomposites					814:827	these nanocomposites	808:827	these nanocomposites	808:827	Stress-transfer mechanisms, between the matrix and the nanowhiskers in both of these nanocomposites are reported.
24188835	7	1	theme	spectral	1091:1098	arg1	peaks					1100:1104	two spectral peaks	1087:1104	two spectral peaks	1087:1104	In order to carry out the latter of these analyses, two spectral peaks are used which correspond to different crystal allomorphs; cellulose-I for the nanowhiskers and cellulose-II for the matrix.
24188835	8	2	theme	tensile	1477:1483	arg1	strength					1485:1492	maximum tensile strength	1469:1492	maximum tensile strength	1469:1492	It is shown that composites comprising a LiCl/DMAc based matrix perform better than NaOH/urea based systems, the T-CNWs provide better reinforcement than CNWs and that an optimum loading of nanowhiskers (at 15 v/v%) is required to obtain maximum tensile strength and modulus.
24188835	4	3	theme	%	692:692	arg1	fraction					674:681	a volume fraction	665:681	a volume fraction of 15 v/v%	665:692	Crystallinity of both nanocomposite systems increases with the addition of nanowhiskers up to a volume fraction of 15 v/v%, after which a plateau is reached.
24188835	6	4	theme	nanowhiskers	978:989	arg1	deformation					954:964	the molecular deformation	940:964	the molecular deformation of both the nanowhiskers and matrix phases	940:1007	This is achieved by following both the mechanical deformation of the materials, and by following the molecular deformation of both the nanowhiskers and matrix phases using Raman spectroscopy.
24188835	3	5	dep	dispersed	391:399	arg1	hydroxide/urea					543:556	sodium hydroxide/urea	536:556	sodium hydroxide/urea (NaOH/urea)	536:568	These nanowhiskers are then dispersed in a cellulose matrix material, produced using two dissolution methods; namely lithium chloride/N,N-dimethyl acetamide (LiCl/DMAc) and sodium hydroxide/urea (NaOH/urea).
24188835	3	5	dep	dispersed	391:399	arg1	NaOH/urea					559:567	NaOH/urea	559:567	NaOH/urea	559:567	These nanowhiskers are then dispersed in a cellulose matrix material, produced using two dissolution methods; namely lithium chloride/N,N-dimethyl acetamide (LiCl/DMAc) and sodium hydroxide/urea (NaOH/urea).
24188835	3	5	dep	dispersed	391:399	arg1	LiCl/DMAc					521:529	LiCl/DMAc	521:529	LiCl/DMAc	521:529	These nanowhiskers are then dispersed in a cellulose matrix material, produced using two dissolution methods; namely lithium chloride/N,N-dimethyl acetamide (LiCl/DMAc) and sodium hydroxide/urea (NaOH/urea).
24188835	3	5	dep	dispersed	391:399	arg1	chloride/N					488:497	lithium chloride/N	480:497	lithium chloride/N	480:497	These nanowhiskers are then dispersed in a cellulose matrix material, produced using two dissolution methods; namely lithium chloride/N,N-dimethyl acetamide (LiCl/DMAc) and sodium hydroxide/urea (NaOH/urea).
24188835	3	5	dep	dispersed	391:399	arg1	acetamide					510:518	N-dimethyl acetamide	499:518	N-dimethyl acetamide (LiCl/DMAc)	499:530	These nanowhiskers are then dispersed in a cellulose matrix material, produced using two dissolution methods; namely lithium chloride/N,N-dimethyl acetamide (LiCl/DMAc) and sodium hydroxide/urea (NaOH/urea).
24188835	8	6	theme	based	1325:1329	arg1	systems					1331:1337	NaOH/urea based systems	1315:1337	NaOH/urea based systems	1315:1337	It is shown that composites comprising a LiCl/DMAc based matrix perform better than NaOH/urea based systems, the T-CNWs provide better reinforcement than CNWs and that an optimum loading of nanowhiskers (at 15 v/v%) is required to obtain maximum tensile strength and modulus.
24188835	4	7	theme	systems	607:613	arg1	Crystallinity					571:583	Crystallinity	571:583	Crystallinity of both nanocomposite systems	571:613	Crystallinity of both nanocomposite systems increases with the addition of nanowhiskers up to a volume fraction of 15 v/v%, after which a plateau is reached.
24188835	8	8	from	%	1444:1444	arg1	nanowhiskers					1421:1432	nanowhiskers	1421:1432	nanowhiskers (at 15 v/v%)	1421:1445	It is shown that composites comprising a LiCl/DMAc based matrix perform better than NaOH/urea based systems, the T-CNWs provide better reinforcement than CNWs and that an optimum loading of nanowhiskers (at 15 v/v%) is required to obtain maximum tensile strength and modulus.
24188835	8	8	from	%	1444:1444	arg1	loading					1410:1416	an optimum loading	1399:1416	an optimum loading of nanowhiskers (at 15 v/v%)	1399:1445	It is shown that composites comprising a LiCl/DMAc based matrix perform better than NaOH/urea based systems, the T-CNWs provide better reinforcement than CNWs and that an optimum loading of nanowhiskers (at 15 v/v%) is required to obtain maximum tensile strength and modulus.
24188835	6	9	theme	mechanical	882:891	arg1	deformation					893:903	the mechanical deformation	878:903	the mechanical deformation of the materials	878:920	This is achieved by following both the mechanical deformation of the materials, and by following the molecular deformation of both the nanowhiskers and matrix phases using Raman spectroscopy.
24188835	8	10	theme	NaOH/urea	1315:1323	arg1	systems					1331:1337	NaOH/urea based systems	1315:1337	NaOH/urea based systems	1315:1337	It is shown that composites comprising a LiCl/DMAc based matrix perform better than NaOH/urea based systems, the T-CNWs provide better reinforcement than CNWs and that an optimum loading of nanowhiskers (at 15 v/v%) is required to obtain maximum tensile strength and modulus.
24188835	2	11	theme	cellulose	302:310	arg1	hydrolysis					254:263	Acid hydrolysis	249:263	Acid hydrolysis of both tunicate (T-CNWs) and cotton cellulose (CNWs)	249:317	Acid hydrolysis of both tunicate (T-CNWs) and cotton cellulose (CNWs) is carried out to produce the nanowhiskers.
24188835	4	12	theme	nanocomposite	593:605	arg1	systems					607:613	both nanocomposite systems	588:613	both nanocomposite systems	588:613	Crystallinity of both nanocomposite systems increases with the addition of nanowhiskers up to a volume fraction of 15 v/v%, after which a plateau is reached.
24188835	8	13	theme	maximum	1469:1475	arg1	strength					1485:1492	maximum tensile strength	1469:1492	maximum tensile strength	1469:1492	It is shown that composites comprising a LiCl/DMAc based matrix perform better than NaOH/urea based systems, the T-CNWs provide better reinforcement than CNWs and that an optimum loading of nanowhiskers (at 15 v/v%) is required to obtain maximum tensile strength and modulus.
24188835	2	14	theme	cotton	295:300	arg1	cellulose					302:310	cotton cellulose	295:310	cotton cellulose (CNWs)	295:317	Acid hydrolysis of both tunicate (T-CNWs) and cotton cellulose (CNWs) is carried out to produce the nanowhiskers.
24188835	2	14	theme	cotton	295:300	arg1	CNWs					313:316	CNWs	313:316	CNWs	313:316	Acid hydrolysis of both tunicate (T-CNWs) and cotton cellulose (CNWs) is carried out to produce the nanowhiskers.
24188835	4	15	theme	volume	667:672	arg1	fraction					674:681	a volume fraction	665:681	a volume fraction of 15 v/v%	665:692	Crystallinity of both nanocomposite systems increases with the addition of nanowhiskers up to a volume fraction of 15 v/v%, after which a plateau is reached.
24188835	0	16	theme	comparing	60:68	arg1	matrix					70:75	comparing matrix and reinforcing components	60:102	matrix	70:75	Deformation micromechanics of all-cellulose nanocomposites: comparing matrix and reinforcing components.
24188835	7	17	theme	analyses	1077:1084	arg1	latter					1061:1066	latter	1061:1066	latter	1061:1066	In order to carry out the latter of these analyses, two spectral peaks are used which correspond to different crystal allomorphs; cellulose-I for the nanowhiskers and cellulose-II for the matrix.
24188835	3	18	theme	sodium	536:541	arg1	hydroxide/urea					543:556	sodium hydroxide/urea	536:556	sodium hydroxide/urea (NaOH/urea)	536:568	These nanowhiskers are then dispersed in a cellulose matrix material, produced using two dissolution methods; namely lithium chloride/N,N-dimethyl acetamide (LiCl/DMAc) and sodium hydroxide/urea (NaOH/urea).
24188835	3	18	theme	sodium	536:541	arg1	NaOH/urea					559:567	NaOH/urea	559:567	NaOH/urea	559:567	These nanowhiskers are then dispersed in a cellulose matrix material, produced using two dissolution methods; namely lithium chloride/N,N-dimethyl acetamide (LiCl/DMAc) and sodium hydroxide/urea (NaOH/urea).
24188835	1	19	theme	different	209:217	arg1	systems					226:232	two different matrix systems	205:232	two different matrix systems	205:232	All-cellulose nanocomposites, comprising two different forms of cellulose nanowhiskers dispersed in two different matrix systems, are produced.
24188835	7	20	theme	crystal	1145:1151	arg1	allomorphs					1153:1162	different crystal allomorphs	1135:1162	different crystal allomorphs	1135:1162	In order to carry out the latter of these analyses, two spectral peaks are used which correspond to different crystal allomorphs; cellulose-I for the nanowhiskers and cellulose-II for the matrix.
24188835	1	21	theme	All-cellulose	105:117	arg1	nanocomposites					119:132	All-cellulose nanocomposites	105:132	All-cellulose nanocomposites	105:132	All-cellulose nanocomposites, comprising two different forms of cellulose nanowhiskers dispersed in two different matrix systems, are produced.
24188835	1	22	theme	matrix	219:224	arg1	systems					226:232	two different matrix systems	205:232	two different matrix systems	205:232	All-cellulose nanocomposites, comprising two different forms of cellulose nanowhiskers dispersed in two different matrix systems, are produced.
24188835	0	23	theme	Deformation	0:10	arg1	micromechanics					12:25	Deformation micromechanics	0:25	Deformation micromechanics of all-cellulose nanocomposites: comparing matrix and reinforcing components.	0:103	Deformation micromechanics of all-cellulose nanocomposites: comparing matrix and reinforcing components.
24188835	8	24	from	loading	1410:1416	arg1	%					1444:1444	15 v/v%	1438:1444	15 v/v%	1438:1444	It is shown that composites comprising a LiCl/DMAc based matrix perform better than NaOH/urea based systems, the T-CNWs provide better reinforcement than CNWs and that an optimum loading of nanowhiskers (at 15 v/v%) is required to obtain maximum tensile strength and modulus.
24188835	7	25	dep	used	1110:1113	arg1	cellulose-II					1202:1213	cellulose-II	1202:1213	cellulose-II	1202:1213	In order to carry out the latter of these analyses, two spectral peaks are used which correspond to different crystal allomorphs; cellulose-I for the nanowhiskers and cellulose-II for the matrix.
24188835	7	25	dep	used	1110:1113	arg1	cellulose-I					1165:1175	cellulose-I	1165:1175	cellulose-I	1165:1175	In order to carry out the latter of these analyses, two spectral peaks are used which correspond to different crystal allomorphs; cellulose-I for the nanowhiskers and cellulose-II for the matrix.
24188835	0	26	theme	all-cellulose	30:42	arg1	nanocomposites					44:57	all-cellulose nanocomposites	30:57	all-cellulose nanocomposites	30:57	Deformation micromechanics of all-cellulose nanocomposites: comparing matrix and reinforcing components.
24188835	8	27	theme	based	1282:1286	arg1	matrix					1288:1293	a LiCl/DMAc based matrix	1270:1293	a LiCl/DMAc based matrix	1270:1293	It is shown that composites comprising a LiCl/DMAc based matrix perform better than NaOH/urea based systems, the T-CNWs provide better reinforcement than CNWs and that an optimum loading of nanowhiskers (at 15 v/v%) is required to obtain maximum tensile strength and modulus.
24188835	8	28	theme	better	1359:1364	arg1	reinforcement					1366:1378	better reinforcement	1359:1378	better reinforcement than CNWs	1359:1388	It is shown that composites comprising a LiCl/DMAc based matrix perform better than NaOH/urea based systems, the T-CNWs provide better reinforcement than CNWs and that an optimum loading of nanowhiskers (at 15 v/v%) is required to obtain maximum tensile strength and modulus.
24188835	4	29	theme	nanowhiskers	646:657	arg1	addition					634:641	the addition	630:641	the addition of nanowhiskers up to a volume fraction of 15 v/v%, after which a plateau is reached	630:726	Crystallinity of both nanocomposite systems increases with the addition of nanowhiskers up to a volume fraction of 15 v/v%, after which a plateau is reached.
24188835	7	30	theme	different	1135:1143	arg1	allomorphs					1153:1162	different crystal allomorphs	1135:1162	different crystal allomorphs	1135:1162	In order to carry out the latter of these analyses, two spectral peaks are used which correspond to different crystal allomorphs; cellulose-I for the nanowhiskers and cellulose-II for the matrix.
24188835	6	31	theme	molecular	944:952	arg1	deformation					954:964	the molecular deformation	940:964	the molecular deformation of both the nanowhiskers and matrix phases	940:1007	This is achieved by following both the mechanical deformation of the materials, and by following the molecular deformation of both the nanowhiskers and matrix phases using Raman spectroscopy.
24188835	8	32	theme	nanowhiskers	1421:1432	arg1	loading					1410:1416	an optimum loading	1399:1416	an optimum loading of nanowhiskers (at 15 v/v%)	1399:1445	It is shown that composites comprising a LiCl/DMAc based matrix perform better than NaOH/urea based systems, the T-CNWs provide better reinforcement than CNWs and that an optimum loading of nanowhiskers (at 15 v/v%) is required to obtain maximum tensile strength and modulus.
24188835	4	33	theme	v/v	689:691	arg1	%					692:692	15 v/v%	686:692	15 v/v%	686:692	Crystallinity of both nanocomposite systems increases with the addition of nanowhiskers up to a volume fraction of 15 v/v%, after which a plateau is reached.
24188835	3	34	theme	matrix	416:421	arg1	material					423:430	a cellulose matrix material	404:430	a cellulose matrix material	404:430	These nanowhiskers are then dispersed in a cellulose matrix material, produced using two dissolution methods; namely lithium chloride/N,N-dimethyl acetamide (LiCl/DMAc) and sodium hydroxide/urea (NaOH/urea).
24188835	3	35	theme	N-dimethyl	499:508	arg1	LiCl/DMAc					521:529	LiCl/DMAc	521:529	LiCl/DMAc	521:529	These nanowhiskers are then dispersed in a cellulose matrix material, produced using two dissolution methods; namely lithium chloride/N,N-dimethyl acetamide (LiCl/DMAc) and sodium hydroxide/urea (NaOH/urea).
24188835	3	35	theme	N-dimethyl	499:508	arg1	acetamide					510:518	N-dimethyl acetamide	499:518	N-dimethyl acetamide (LiCl/DMAc)	499:530	These nanowhiskers are then dispersed in a cellulose matrix material, produced using two dissolution methods; namely lithium chloride/N,N-dimethyl acetamide (LiCl/DMAc) and sodium hydroxide/urea (NaOH/urea).
24188835	3	36	theme	dissolution	452:462	arg1	methods					464:470	two dissolution methods	448:470	two dissolution methods	448:470	These nanowhiskers are then dispersed in a cellulose matrix material, produced using two dissolution methods; namely lithium chloride/N,N-dimethyl acetamide (LiCl/DMAc) and sodium hydroxide/urea (NaOH/urea).
24188835	0	37	theme	nanocomposites	44:57	arg1	micromechanics					12:25	Deformation micromechanics	0:25	Deformation micromechanics of all-cellulose nanocomposites: comparing matrix and reinforcing components.	0:103	Deformation micromechanics of all-cellulose nanocomposites: comparing matrix and reinforcing components.
24188835	5	38	theme	Stress-transfer	729:743	arg1	mechanisms					745:754	Stress-transfer mechanisms	729:754	Stress-transfer mechanisms	729:754	Stress-transfer mechanisms, between the matrix and the nanowhiskers in both of these nanocomposites are reported.
24188835	7	39	used	used	1110:1113	arg2	peaks					1100:1104	two spectral peaks	1087:1104	two spectral peaks	1087:1104	In order to carry out the latter of these analyses, two spectral peaks are used which correspond to different crystal allomorphs; cellulose-I for the nanowhiskers and cellulose-II for the matrix.
24188835	3	40	theme	cellulose	406:414	arg1	material					423:430	a cellulose matrix material	404:430	a cellulose matrix material	404:430	These nanowhiskers are then dispersed in a cellulose matrix material, produced using two dissolution methods; namely lithium chloride/N,N-dimethyl acetamide (LiCl/DMAc) and sodium hydroxide/urea (NaOH/urea).
24188835	8	41	theme	v/v	1441:1443	arg1	%					1444:1444	15 v/v%	1438:1444	15 v/v%	1438:1444	It is shown that composites comprising a LiCl/DMAc based matrix perform better than NaOH/urea based systems, the T-CNWs provide better reinforcement than CNWs and that an optimum loading of nanowhiskers (at 15 v/v%) is required to obtain maximum tensile strength and modulus.
24188835	2	42	theme	Acid	249:252	arg1	hydrolysis					254:263	Acid hydrolysis	249:263	Acid hydrolysis of both tunicate (T-CNWs) and cotton cellulose (CNWs)	249:317	Acid hydrolysis of both tunicate (T-CNWs) and cotton cellulose (CNWs) is carried out to produce the nanowhiskers.
24188835	1	43	theme	different	150:158	arg1	forms					160:164	two different forms	146:164	two different forms of cellulose nanowhiskers dispersed in two different matrix systems	146:232	All-cellulose nanocomposites, comprising two different forms of cellulose nanowhiskers dispersed in two different matrix systems, are produced.
24188835	8	44	theme	optimum	1402:1408	arg1	loading					1410:1416	an optimum loading	1399:1416	an optimum loading of nanowhiskers (at 15 v/v%)	1399:1445	It is shown that composites comprising a LiCl/DMAc based matrix perform better than NaOH/urea based systems, the T-CNWs provide better reinforcement than CNWs and that an optimum loading of nanowhiskers (at 15 v/v%) is required to obtain maximum tensile strength and modulus.
24188835	3	45	theme	lithium	480:486	arg1	chloride/N					488:497	lithium chloride/N	480:497	lithium chloride/N	480:497	These nanowhiskers are then dispersed in a cellulose matrix material, produced using two dissolution methods; namely lithium chloride/N,N-dimethyl acetamide (LiCl/DMAc) and sodium hydroxide/urea (NaOH/urea).
24188835	6	46	theme	Raman	1015:1019	arg1	spectroscopy					1021:1032	Raman spectroscopy	1015:1032	Raman spectroscopy	1015:1032	This is achieved by following both the mechanical deformation of the materials, and by following the molecular deformation of both the nanowhiskers and matrix phases using Raman spectroscopy.
24188835	5	47	from	mechanisms	745:754	arg1	both					800:803	both	800:803	both	800:803	Stress-transfer mechanisms, between the matrix and the nanowhiskers in both of these nanocomposites are reported.
24188835	5	47	from	mechanisms	745:754	arg1	nanocomposites					814:827	these nanocomposites	808:827	these nanocomposites	808:827	Stress-transfer mechanisms, between the matrix and the nanowhiskers in both of these nanocomposites are reported.
24188835	4	48	dep	fraction	674:681	arg1	up					659:660	up	659:660	up	659:660	Crystallinity of both nanocomposite systems increases with the addition of nanowhiskers up to a volume fraction of 15 v/v%, after which a plateau is reached.
24188835	8	49	theme	LiCl/DMAc	1272:1280	arg1	matrix					1288:1293	a LiCl/DMAc based matrix	1270:1293	a LiCl/DMAc based matrix	1270:1293	It is shown that composites comprising a LiCl/DMAc based matrix perform better than NaOH/urea based systems, the T-CNWs provide better reinforcement than CNWs and that an optimum loading of nanowhiskers (at 15 v/v%) is required to obtain maximum tensile strength and modulus.
24188835	6	50	dep	following	863:871	arg1	both					873:876	both	873:876	both	873:876	This is achieved by following both the mechanical deformation of the materials, and by following the molecular deformation of both the nanowhiskers and matrix phases using Raman spectroscopy.
24188835	0	51	theme	reinforcing	81:91	arg1	components					93:102	comparing matrix and reinforcing components	60:102	components	93:102	Deformation micromechanics of all-cellulose nanocomposites: comparing matrix and reinforcing components.
24188835	0	52	dep	micromechanics	12:25	arg1	components					93:102	comparing matrix and reinforcing components	60:102	components	93:102	Deformation micromechanics of all-cellulose nanocomposites: comparing matrix and reinforcing components.
24188835	0	52	dep	micromechanics	12:25	arg1	matrix					70:75	comparing matrix and reinforcing components	60:102	matrix	70:75	Deformation micromechanics of all-cellulose nanocomposites: comparing matrix and reinforcing components.
24188835	1	53	theme	cellulose	169:177	arg1	nanowhiskers					179:190	cellulose nanowhiskers	169:190	cellulose nanowhiskers dispersed in two different matrix systems	169:232	All-cellulose nanocomposites, comprising two different forms of cellulose nanowhiskers dispersed in two different matrix systems, are produced.
24188835	6	54	theme	phases	1002:1007	arg1	deformation					954:964	the molecular deformation	940:964	the molecular deformation of both the nanowhiskers and matrix phases	940:1007	This is achieved by following both the mechanical deformation of the materials, and by following the molecular deformation of both the nanowhiskers and matrix phases using Raman spectroscopy.
24188835	6	55	theme	materials	912:920	arg1	following					863:871	following	863:871	following	863:871	This is achieved by following both the mechanical deformation of the materials, and by following the molecular deformation of both the nanowhiskers and matrix phases using Raman spectroscopy.
24188835	6	55	theme	materials	912:920	arg1	deformation					893:903	the mechanical deformation	878:903	the mechanical deformation of the materials	878:920	This is achieved by following both the mechanical deformation of the materials, and by following the molecular deformation of both the nanowhiskers and matrix phases using Raman spectroscopy.
24188835	2	56	theme	tunicate	273:280	arg1	hydrolysis					254:263	Acid hydrolysis	249:263	Acid hydrolysis of both tunicate (T-CNWs) and cotton cellulose (CNWs)	249:317	Acid hydrolysis of both tunicate (T-CNWs) and cotton cellulose (CNWs) is carried out to produce the nanowhiskers.
24188835	1	57	theme	nanowhiskers	179:190	arg1	forms					160:164	two different forms	146:164	two different forms of cellulose nanowhiskers dispersed in two different matrix systems	146:232	All-cellulose nanocomposites, comprising two different forms of cellulose nanowhiskers dispersed in two different matrix systems, are produced.
24188835	6	58	theme	matrix	995:1000	arg1	phases					1002:1007	matrix phases	995:1007	matrix phases	995:1007	This is achieved by following both the mechanical deformation of the materials, and by following the molecular deformation of both the nanowhiskers and matrix phases using Raman spectroscopy.
26362834	8	0	theme	commercial	1405:1414	arg1	plant					1431:1435	C4 plant	1428:1435	C4 plant	1428:1435	CONCLUSION The (13) C NMR method is a good alternative to complex isotope ratio mass spectrometry measurements for routine detection and semi-quantification of adulteration of commercial cane sugar (C4 plant) with less expensive beet sugar (C3 plant).
26362834	8	0	theme	commercial	1405:1414	arg1	sugar					1421:1425	commercial cane sugar	1405:1425	commercial cane sugar (C4 plant)	1405:1436	CONCLUSION The (13) C NMR method is a good alternative to complex isotope ratio mass spectrometry measurements for routine detection and semi-quantification of adulteration of commercial cane sugar (C4 plant) with less expensive beet sugar (C3 plant).
26362834	6	1	theme	different	1046:1054	arg1	origin					1066:1071	different botanical origin	1046:1071	different botanical origin	1046:1071	The method was applied to ten commercial sucrose-based sugar products of different botanical origin.
26362834	8	2	theme	beet	1458:1461	arg1	plant					1473:1477	less expensive beet sugar (C3 plant)	1443:1478	less expensive beet sugar (C3 plant)	1443:1478	CONCLUSION The (13) C NMR method is a good alternative to complex isotope ratio mass spectrometry measurements for routine detection and semi-quantification of adulteration of commercial cane sugar (C4 plant) with less expensive beet sugar (C3 plant).
26362834	5	3	theme	sugar	966:970	arg1	%					956:956	0 to 100 wt%	945:956	0 to 100 wt% of beet sugar	945:970	Good linearity (R(2) = 0.93) was obtained for the beet sugar-cane sugar blends containing from 0 to 100 wt% of beet sugar.
26362834	5	3	theme	sugar	966:970	arg1	sugar					966:970	beet sugar	961:970	beet sugar	961:970	Good linearity (R(2) = 0.93) was obtained for the beet sugar-cane sugar blends containing from 0 to 100 wt% of beet sugar.
26362834	2	4	theme	C4	517:518	arg1	plant					520:524	C4 plant	517:524	C4 plant	517:524	RESULTS A direct (13) C nuclear magnetic resonance (NMR) method has been validated to differentiate between sucrose-based sugar products produced from sugar beet (C3 plant) and sugar cane (C4 plant).
26362834	2	4	theme	C4	517:518	arg1	cane					511:514	sugar cane	505:514	sugar cane (C4 plant)	505:525	RESULTS A direct (13) C nuclear magnetic resonance (NMR) method has been validated to differentiate between sucrose-based sugar products produced from sugar beet (C3 plant) and sugar cane (C4 plant).
26362834	8	5	theme	sugar	1421:1425	arg1	adulteration					1389:1400	adulteration	1389:1400	adulteration of commercial cane sugar (C4 plant) with less expensive beet sugar (C3 plant)	1389:1478	CONCLUSION The (13) C NMR method is a good alternative to complex isotope ratio mass spectrometry measurements for routine detection and semi-quantification of adulteration of commercial cane sugar (C4 plant) with less expensive beet sugar (C3 plant).
26362834	1	6	theme	simple	229:234	arg1	methodology					247:257	a simple analytical methodology	227:257	a simple analytical methodology	227:257	BACKGROUND Due to possible falsification of sugar cane products with cheaper alternative (sugar beet) on the market, a simple analytical methodology needs to be developed to control the authenticity of sugar products.
26362834	6	7	theme	sucrose-based	1014:1026	arg1	products					1034:1041	ten commercial sucrose-based sugar products	999:1041	ten commercial sucrose-based sugar products of different botanical origin	999:1071	The method was applied to ten commercial sucrose-based sugar products of different botanical origin.
26362834	1	8	theme	cane	160:163	arg1	products					165:172	sugar cane products	154:172	sugar cane products	154:172	BACKGROUND Due to possible falsification of sugar cane products with cheaper alternative (sugar beet) on the market, a simple analytical methodology needs to be developed to control the authenticity of sugar products.
26362834	2	9	theme	sugar	505:509	arg1	plant					520:524	C4 plant	517:524	C4 plant	517:524	RESULTS A direct (13) C nuclear magnetic resonance (NMR) method has been validated to differentiate between sucrose-based sugar products produced from sugar beet (C3 plant) and sugar cane (C4 plant).
26362834	2	9	theme	sugar	505:509	arg1	cane					511:514	sugar cane	505:514	sugar cane (C4 plant)	505:525	RESULTS A direct (13) C nuclear magnetic resonance (NMR) method has been validated to differentiate between sucrose-based sugar products produced from sugar beet (C3 plant) and sugar cane (C4 plant).
26362834	4	10	dep	reach	787:791	arg1	%					846:846	<0.2%	842:846	<0.2%	842:846	NMR acquisition parameters and data processing have been optimized to reach a high level of intraday and interday precision (<0.2%).
26362834	1	11	theme	sugar	200:204	arg1	alternative					187:197	cheaper alternative	179:197	cheaper alternative (sugar beet)	179:210	BACKGROUND Due to possible falsification of sugar cane products with cheaper alternative (sugar beet) on the market, a simple analytical methodology needs to be developed to control the authenticity of sugar products.
26362834	1	11	theme	sugar	200:204	arg1	beet					206:209	sugar beet	200:209	sugar beet	200:209	BACKGROUND Due to possible falsification of sugar cane products with cheaper alternative (sugar beet) on the market, a simple analytical methodology needs to be developed to control the authenticity of sugar products.
26362834	4	12	theme	acquisition	721:731	arg1	parameters					733:742	NMR acquisition parameters	717:742	NMR acquisition parameters	717:742	NMR acquisition parameters and data processing have been optimized to reach a high level of intraday and interday precision (<0.2%).
26362834	7	13	theme	relative	1128:1135	arg1	areas					1142:1146	the relative peak areas	1124:1146	the relative peak areas for replicate measurements	1124:1173	Principal component analysis (PCA) was applied to the relative peak areas for replicate measurements to visualize the difference between studied products.
26362834	5	14	theme	Good	850:853	arg1	R					866:866	R(2) = 0.93	866:876	R(2) = 0.93	866:876	Good linearity (R(2) = 0.93) was obtained for the beet sugar-cane sugar blends containing from 0 to 100 wt% of beet sugar.
26362834	5	14	theme	Good	850:853	arg1	linearity					855:863	Good linearity	850:863	Good linearity (R(2) = 0.93)	850:877	Good linearity (R(2) = 0.93) was obtained for the beet sugar-cane sugar blends containing from 0 to 100 wt% of beet sugar.
26362834	3	15	theme	positions	629:637	arg1	content					579:585	relative (13) C content	563:585	relative (13) C content of the C1, C2, C5, and the C1, C4, C5, C6 positions of the glycosyl and fructosyl moieties of the sucrose molecule, respectively	563:714	The method is based on calculating relative (13) C content of the C1, C2, C5, and the C1, C4, C5, C6 positions of the glycosyl and fructosyl moieties of the sucrose molecule, respectively.
26362834	6	16	theme	origin	1066:1071	arg1	products					1034:1041	ten commercial sucrose-based sugar products	999:1041	ten commercial sucrose-based sugar products of different botanical origin	999:1071	The method was applied to ten commercial sucrose-based sugar products of different botanical origin.
26362834	1	17	theme	Due	121:123	arg1	BACKGROUND					110:119	BACKGROUND	110:119	BACKGROUND Due to possible falsification of sugar cane products with cheaper alternative (sugar beet) on the market, a simple analytical methodology needs to be developed to control the authenticity of sugar products.	110:326	BACKGROUND Due to possible falsification of sugar cane products with cheaper alternative (sugar beet) on the market, a simple analytical methodology needs to be developed to control the authenticity of sugar products.
26362834	3	18	theme	moieties	669:676	arg1	positions					629:637	the C1, C2, C5, and the C1, C4, C5, C6 positions	590:637	the C1, C2, C5, and the C1, C4, C5, C6 positions	590:637	The method is based on calculating relative (13) C content of the C1, C2, C5, and the C1, C4, C5, C6 positions of the glycosyl and fructosyl moieties of the sucrose molecule, respectively.
26362834	2	19	theme	sugar	450:454	arg1	products					456:463	sucrose-based sugar products	436:463	sucrose-based sugar products produced from sugar beet (C3 plant) and sugar cane (C4 plant)	436:525	RESULTS A direct (13) C nuclear magnetic resonance (NMR) method has been validated to differentiate between sucrose-based sugar products produced from sugar beet (C3 plant) and sugar cane (C4 plant).
26362834	7	20	dep	measurements	1162:1173	arg1	replicate					1152:1160	replicate	1152:1160	replicate	1152:1160	Principal component analysis (PCA) was applied to the relative peak areas for replicate measurements to visualize the difference between studied products.
26362834	4	21	theme	intraday	809:816	arg1	level					800:804	a high level	793:804	a high level of intraday and interday precision	793:839	NMR acquisition parameters and data processing have been optimized to reach a high level of intraday and interday precision (<0.2%).
26362834	8	22	theme	NMR	1251:1253	arg1	alternative					1272:1282	a good alternative	1265:1282	a good alternative to complex isotope ratio mass spectrometry measurements for routine detection and semi-quantification of adulteration of commercial cane sugar (C4 plant) with less expensive beet sugar (C3 plant)	1265:1478	CONCLUSION The (13) C NMR method is a good alternative to complex isotope ratio mass spectrometry measurements for routine detection and semi-quantification of adulteration of commercial cane sugar (C4 plant) with less expensive beet sugar (C3 plant).
26362834	8	22	theme	NMR	1251:1253	arg1	method					1255:1260	The (13) C NMR method	1240:1260	The (13) C NMR method	1240:1260	CONCLUSION The (13) C NMR method is a good alternative to complex isotope ratio mass spectrometry measurements for routine detection and semi-quantification of adulteration of commercial cane sugar (C4 plant) with less expensive beet sugar (C3 plant).
26362834	0	23	theme	natural	80:86	arg1	NMR					105:107	quantitative natural abundance (13) C NMR	67:107	quantitative natural abundance (13) C NMR	67:107	Authentication of the origin of sucrose-based sugar products using quantitative natural abundance (13) C NMR.
26362834	5	24	theme	sugar-cane	905:914	arg1	blends					922:927	the beet sugar-cane sugar blends	896:927	the beet sugar-cane sugar blends containing from 0 to 100 wt% of beet sugar	896:970	Good linearity (R(2) = 0.93) was obtained for the beet sugar-cane sugar blends containing from 0 to 100 wt% of beet sugar.
26362834	3	25	theme	molecule	693:700	arg1	moieties					669:676	the glycosyl and fructosyl moieties	642:676	moieties	669:676	The method is based on calculating relative (13) C content of the C1, C2, C5, and the C1, C4, C5, C6 positions of the glycosyl and fructosyl moieties of the sucrose molecule, respectively.
26362834	2	26	theme	sugar	479:483	arg1	beet					485:488	sugar beet	479:488	sugar beet (C3 plant)	479:499	RESULTS A direct (13) C nuclear magnetic resonance (NMR) method has been validated to differentiate between sucrose-based sugar products produced from sugar beet (C3 plant) and sugar cane (C4 plant).
26362834	2	26	theme	sugar	479:483	arg1	plant					494:498	C3 plant	491:498	C3 plant	491:498	RESULTS A direct (13) C nuclear magnetic resonance (NMR) method has been validated to differentiate between sucrose-based sugar products produced from sugar beet (C3 plant) and sugar cane (C4 plant).
26362834	5	27	dep	100 wt	950:955	arg1	to					947:948	to	947:948	to	947:948	Good linearity (R(2) = 0.93) was obtained for the beet sugar-cane sugar blends containing from 0 to 100 wt% of beet sugar.
26362834	1	28	dep	BACKGROUND	110:119	arg1	needs					259:263	needs	259:263	needs to be developed to control the authenticity of sugar products	259:325	BACKGROUND Due to possible falsification of sugar cane products with cheaper alternative (sugar beet) on the market, a simple analytical methodology needs to be developed to control the authenticity of sugar products.
26362834	1	29	theme	products	318:325	arg1	authenticity					296:307	the authenticity	292:307	the authenticity of sugar products	292:325	BACKGROUND Due to possible falsification of sugar cane products with cheaper alternative (sugar beet) on the market, a simple analytical methodology needs to be developed to control the authenticity of sugar products.
26362834	8	30	theme	good	1267:1270	arg1	alternative					1272:1282	a good alternative	1265:1282	a good alternative to complex isotope ratio mass spectrometry measurements for routine detection and semi-quantification of adulteration of commercial cane sugar (C4 plant) with less expensive beet sugar (C3 plant)	1265:1478	CONCLUSION The (13) C NMR method is a good alternative to complex isotope ratio mass spectrometry measurements for routine detection and semi-quantification of adulteration of commercial cane sugar (C4 plant) with less expensive beet sugar (C3 plant).
26362834	8	30	theme	good	1267:1270	arg1	method					1255:1260	The (13) C NMR method	1240:1260	The (13) C NMR method	1240:1260	CONCLUSION The (13) C NMR method is a good alternative to complex isotope ratio mass spectrometry measurements for routine detection and semi-quantification of adulteration of commercial cane sugar (C4 plant) with less expensive beet sugar (C3 plant).
26362834	1	31	theme	analytical	236:245	arg1	methodology					247:257	a simple analytical methodology	227:257	a simple analytical methodology	227:257	BACKGROUND Due to possible falsification of sugar cane products with cheaper alternative (sugar beet) on the market, a simple analytical methodology needs to be developed to control the authenticity of sugar products.
26362834	8	32	theme	isotope	1295:1301	arg1	spectrometry					1314:1325	complex isotope ratio mass spectrometry	1287:1325	complex isotope ratio mass spectrometry measurements for routine detection and semi-quantification of adulteration of commercial cane sugar (C4 plant) with less expensive beet sugar (C3 plant)	1287:1478	CONCLUSION The (13) C NMR method is a good alternative to complex isotope ratio mass spectrometry measurements for routine detection and semi-quantification of adulteration of commercial cane sugar (C4 plant) with less expensive beet sugar (C3 plant).
26362834	2	33	theme	resonance	369:377	arg1	method					385:390	A direct (13) C nuclear magnetic resonance (NMR) method	336:390	A direct (13) C nuclear magnetic resonance (NMR) method	336:390	RESULTS A direct (13) C nuclear magnetic resonance (NMR) method has been validated to differentiate between sucrose-based sugar products produced from sugar beet (C3 plant) and sugar cane (C4 plant).
26362834	7	34	theme	component	1084:1092	arg1	PCA					1104:1106	PCA	1104:1106	PCA	1104:1106	Principal component analysis (PCA) was applied to the relative peak areas for replicate measurements to visualize the difference between studied products.
26362834	7	34	theme	component	1084:1092	arg1	analysis					1094:1101	Principal component analysis	1074:1101	Principal component analysis (PCA)	1074:1107	Principal component analysis (PCA) was applied to the relative peak areas for replicate measurements to visualize the difference between studied products.
26362834	4	35	theme	interday	822:829	arg1	level					800:804	a high level	793:804	a high level of intraday and interday precision	793:839	NMR acquisition parameters and data processing have been optimized to reach a high level of intraday and interday precision (<0.2%).
26362834	8	36	theme	mass	1309:1312	arg1	spectrometry					1314:1325	complex isotope ratio mass spectrometry	1287:1325	complex isotope ratio mass spectrometry measurements for routine detection and semi-quantification of adulteration of commercial cane sugar (C4 plant) with less expensive beet sugar (C3 plant)	1287:1478	CONCLUSION The (13) C NMR method is a good alternative to complex isotope ratio mass spectrometry measurements for routine detection and semi-quantification of adulteration of commercial cane sugar (C4 plant) with less expensive beet sugar (C3 plant).
26362834	3	37	theme	relative	563:570	arg1	content					579:585	relative (13) C content	563:585	relative (13) C content of the C1, C2, C5, and the C1, C4, C5, C6 positions of the glycosyl and fructosyl moieties of the sucrose molecule, respectively	563:714	The method is based on calculating relative (13) C content of the C1, C2, C5, and the C1, C4, C5, C6 positions of the glycosyl and fructosyl moieties of the sucrose molecule, respectively.
26362834	2	38	theme	nuclear	352:358	arg1	NMR					380:382	NMR	380:382	NMR	380:382	RESULTS A direct (13) C nuclear magnetic resonance (NMR) method has been validated to differentiate between sucrose-based sugar products produced from sugar beet (C3 plant) and sugar cane (C4 plant).
26362834	2	38	theme	nuclear	352:358	arg1	resonance					369:377	direct (13) C nuclear magnetic resonance	338:377	A direct (13) C nuclear magnetic resonance (NMR) method	336:390	RESULTS A direct (13) C nuclear magnetic resonance (NMR) method has been validated to differentiate between sucrose-based sugar products produced from sugar beet (C3 plant) and sugar cane (C4 plant).
26362834	2	39	theme	sucrose-based	436:448	arg1	products					456:463	sucrose-based sugar products	436:463	sucrose-based sugar products produced from sugar beet (C3 plant) and sugar cane (C4 plant)	436:525	RESULTS A direct (13) C nuclear magnetic resonance (NMR) method has been validated to differentiate between sucrose-based sugar products produced from sugar beet (C3 plant) and sugar cane (C4 plant).
26362834	0	40	theme	sugar	46:50	arg1	products					52:59	sucrose-based sugar products	32:59	sucrose-based sugar products	32:59	Authentication of the origin of sucrose-based sugar products using quantitative natural abundance (13) C NMR.
26362834	8	41	theme	routine	1344:1350	arg1	detection					1352:1360	routine detection	1344:1360	routine detection	1344:1360	CONCLUSION The (13) C NMR method is a good alternative to complex isotope ratio mass spectrometry measurements for routine detection and semi-quantification of adulteration of commercial cane sugar (C4 plant) with less expensive beet sugar (C3 plant).
26362834	3	42	theme	C	577:577	arg1	content					579:585	relative (13) C content	563:585	relative (13) C content of the C1, C2, C5, and the C1, C4, C5, C6 positions of the glycosyl and fructosyl moieties of the sucrose molecule, respectively	563:714	The method is based on calculating relative (13) C content of the C1, C2, C5, and the C1, C4, C5, C6 positions of the glycosyl and fructosyl moieties of the sucrose molecule, respectively.
26362834	1	43	theme	possible	128:135	arg1	falsification					137:149	possible falsification	128:149	possible falsification of sugar cane products with cheaper alternative (sugar beet)	128:210	BACKGROUND Due to possible falsification of sugar cane products with cheaper alternative (sugar beet) on the market, a simple analytical methodology needs to be developed to control the authenticity of sugar products.
26362834	3	44	theme	C1	594:595	arg1	positions					629:637	the C1, C2, C5, and the C1, C4, C5, C6 positions	590:637	the C1, C2, C5, and the C1, C4, C5, C6 positions	590:637	The method is based on calculating relative (13) C content of the C1, C2, C5, and the C1, C4, C5, C6 positions of the glycosyl and fructosyl moieties of the sucrose molecule, respectively.
26362834	6	45	theme	botanical	1056:1064	arg1	origin					1066:1071	different botanical origin	1046:1071	different botanical origin	1046:1071	The method was applied to ten commercial sucrose-based sugar products of different botanical origin.
26362834	5	46	theme	beet	961:964	arg1	sugar					966:970	beet sugar	961:970	beet sugar	961:970	Good linearity (R(2) = 0.93) was obtained for the beet sugar-cane sugar blends containing from 0 to 100 wt% of beet sugar.
26362834	8	47	with	adulteration	1389:1400	arg1	plant					1473:1477	less expensive beet sugar (C3 plant)	1443:1478	less expensive beet sugar (C3 plant)	1443:1478	CONCLUSION The (13) C NMR method is a good alternative to complex isotope ratio mass spectrometry measurements for routine detection and semi-quantification of adulteration of commercial cane sugar (C4 plant) with less expensive beet sugar (C3 plant).
26362834	8	48	theme	expensive	1448:1456	arg1	plant					1473:1477	less expensive beet sugar (C3 plant)	1443:1478	less expensive beet sugar (C3 plant)	1443:1478	CONCLUSION The (13) C NMR method is a good alternative to complex isotope ratio mass spectrometry measurements for routine detection and semi-quantification of adulteration of commercial cane sugar (C4 plant) with less expensive beet sugar (C3 plant).
26362834	7	49	theme	studied	1211:1217	arg1	products					1219:1226	studied products	1211:1226	studied products	1211:1226	Principal component analysis (PCA) was applied to the relative peak areas for replicate measurements to visualize the difference between studied products.
26362834	8	50	theme	cane	1416:1419	arg1	plant					1431:1435	C4 plant	1428:1435	C4 plant	1428:1435	CONCLUSION The (13) C NMR method is a good alternative to complex isotope ratio mass spectrometry measurements for routine detection and semi-quantification of adulteration of commercial cane sugar (C4 plant) with less expensive beet sugar (C3 plant).
26362834	8	50	theme	cane	1416:1419	arg1	sugar					1421:1425	commercial cane sugar	1405:1425	commercial cane sugar (C4 plant)	1405:1436	CONCLUSION The (13) C NMR method is a good alternative to complex isotope ratio mass spectrometry measurements for routine detection and semi-quantification of adulteration of commercial cane sugar (C4 plant) with less expensive beet sugar (C3 plant).
26362834	3	51	theme	C5	602:603	arg1	positions					629:637	the C1, C2, C5, and the C1, C4, C5, C6 positions	590:637	the C1, C2, C5, and the C1, C4, C5, C6 positions	590:637	The method is based on calculating relative (13) C content of the C1, C2, C5, and the C1, C4, C5, C6 positions of the glycosyl and fructosyl moieties of the sucrose molecule, respectively.
26362834	6	52	theme	sugar	1028:1032	arg1	products					1034:1041	ten commercial sucrose-based sugar products	999:1041	ten commercial sucrose-based sugar products of different botanical origin	999:1071	The method was applied to ten commercial sucrose-based sugar products of different botanical origin.
26362834	8	53	theme	sugar	1463:1467	arg1	plant					1473:1477	less expensive beet sugar (C3 plant)	1443:1478	less expensive beet sugar (C3 plant)	1443:1478	CONCLUSION The (13) C NMR method is a good alternative to complex isotope ratio mass spectrometry measurements for routine detection and semi-quantification of adulteration of commercial cane sugar (C4 plant) with less expensive beet sugar (C3 plant).
26362834	1	54	theme	sugar	154:158	arg1	products					165:172	sugar cane products	154:172	sugar cane products	154:172	BACKGROUND Due to possible falsification of sugar cane products with cheaper alternative (sugar beet) on the market, a simple analytical methodology needs to be developed to control the authenticity of sugar products.
26362834	3	55	theme	C2	598:599	arg1	positions					629:637	the C1, C2, C5, and the C1, C4, C5, C6 positions	590:637	the C1, C2, C5, and the C1, C4, C5, C6 positions	590:637	The method is based on calculating relative (13) C content of the C1, C2, C5, and the C1, C4, C5, C6 positions of the glycosyl and fructosyl moieties of the sucrose molecule, respectively.
26362834	6	56	theme	commercial	1003:1012	arg1	products					1034:1041	ten commercial sucrose-based sugar products	999:1041	ten commercial sucrose-based sugar products of different botanical origin	999:1071	The method was applied to ten commercial sucrose-based sugar products of different botanical origin.
26362834	8	57	theme	C3	1470:1471	arg1	plant					1473:1477	less expensive beet sugar (C3 plant)	1443:1478	less expensive beet sugar (C3 plant)	1443:1478	CONCLUSION The (13) C NMR method is a good alternative to complex isotope ratio mass spectrometry measurements for routine detection and semi-quantification of adulteration of commercial cane sugar (C4 plant) with less expensive beet sugar (C3 plant).
26362834	1	58	theme	products	165:172	arg1	falsification					137:149	possible falsification	128:149	possible falsification of sugar cane products with cheaper alternative (sugar beet)	128:210	BACKGROUND Due to possible falsification of sugar cane products with cheaper alternative (sugar beet) on the market, a simple analytical methodology needs to be developed to control the authenticity of sugar products.
26362834	3	59	theme	C1	614:615	arg1	positions					629:637	the C1, C2, C5, and the C1, C4, C5, C6 positions	590:637	the C1, C2, C5, and the C1, C4, C5, C6 positions	590:637	The method is based on calculating relative (13) C content of the C1, C2, C5, and the C1, C4, C5, C6 positions of the glycosyl and fructosyl moieties of the sucrose molecule, respectively.
26362834	1	60	theme	cheaper	179:185	arg1	alternative					187:197	cheaper alternative	179:197	cheaper alternative (sugar beet)	179:210	BACKGROUND Due to possible falsification of sugar cane products with cheaper alternative (sugar beet) on the market, a simple analytical methodology needs to be developed to control the authenticity of sugar products.
26362834	1	60	theme	cheaper	179:185	arg1	beet					206:209	sugar beet	200:209	sugar beet	200:209	BACKGROUND Due to possible falsification of sugar cane products with cheaper alternative (sugar beet) on the market, a simple analytical methodology needs to be developed to control the authenticity of sugar products.
26362834	2	61	theme	C3	491:492	arg1	beet					485:488	sugar beet	479:488	sugar beet (C3 plant)	479:499	RESULTS A direct (13) C nuclear magnetic resonance (NMR) method has been validated to differentiate between sucrose-based sugar products produced from sugar beet (C3 plant) and sugar cane (C4 plant).
26362834	2	61	theme	C3	491:492	arg1	plant					494:498	C3 plant	491:498	C3 plant	491:498	RESULTS A direct (13) C nuclear magnetic resonance (NMR) method has been validated to differentiate between sucrose-based sugar products produced from sugar beet (C3 plant) and sugar cane (C4 plant).
26362834	4	62	dep	intraday	809:816	arg1	precision					831:839	precision	831:839	precision	831:839	NMR acquisition parameters and data processing have been optimized to reach a high level of intraday and interday precision (<0.2%).
26362834	5	63	theme	sugar	916:920	arg1	blends					922:927	the beet sugar-cane sugar blends	896:927	the beet sugar-cane sugar blends containing from 0 to 100 wt% of beet sugar	896:970	Good linearity (R(2) = 0.93) was obtained for the beet sugar-cane sugar blends containing from 0 to 100 wt% of beet sugar.
26362834	4	64	theme	NMR	717:719	arg1	parameters					733:742	NMR acquisition parameters	717:742	NMR acquisition parameters	717:742	NMR acquisition parameters and data processing have been optimized to reach a high level of intraday and interday precision (<0.2%).
26362834	8	65	theme	13	1245:1246	arg1	C					1249:1249	C	1249:1249	C	1249:1249	CONCLUSION The (13) C NMR method is a good alternative to complex isotope ratio mass spectrometry measurements for routine detection and semi-quantification of adulteration of commercial cane sugar (C4 plant) with less expensive beet sugar (C3 plant).
26362834	7	66	theme	peak	1137:1140	arg1	areas					1142:1146	the relative peak areas	1124:1146	the relative peak areas for replicate measurements	1124:1173	Principal component analysis (PCA) was applied to the relative peak areas for replicate measurements to visualize the difference between studied products.
26362834	3	67	theme	C4	618:619	arg1	positions					629:637	the C1, C2, C5, and the C1, C4, C5, C6 positions	590:637	the C1, C2, C5, and the C1, C4, C5, C6 positions	590:637	The method is based on calculating relative (13) C content of the C1, C2, C5, and the C1, C4, C5, C6 positions of the glycosyl and fructosyl moieties of the sucrose molecule, respectively.
26362834	3	68	theme	sucrose	685:691	arg1	molecule					693:700	the sucrose molecule	681:700	the sucrose molecule	681:700	The method is based on calculating relative (13) C content of the C1, C2, C5, and the C1, C4, C5, C6 positions of the glycosyl and fructosyl moieties of the sucrose molecule, respectively.
26362834	8	69	theme	C	1249:1249	arg1	alternative					1272:1282	a good alternative	1265:1282	a good alternative to complex isotope ratio mass spectrometry measurements for routine detection and semi-quantification of adulteration of commercial cane sugar (C4 plant) with less expensive beet sugar (C3 plant)	1265:1478	CONCLUSION The (13) C NMR method is a good alternative to complex isotope ratio mass spectrometry measurements for routine detection and semi-quantification of adulteration of commercial cane sugar (C4 plant) with less expensive beet sugar (C3 plant).
26362834	8	69	theme	C	1249:1249	arg1	method					1255:1260	The (13) C NMR method	1240:1260	The (13) C NMR method	1240:1260	CONCLUSION The (13) C NMR method is a good alternative to complex isotope ratio mass spectrometry measurements for routine detection and semi-quantification of adulteration of commercial cane sugar (C4 plant) with less expensive beet sugar (C3 plant).
26362834	0	70	theme	quantitative	67:78	arg1	NMR					105:107	quantitative natural abundance (13) C NMR	67:107	quantitative natural abundance (13) C NMR	67:107	Authentication of the origin of sucrose-based sugar products using quantitative natural abundance (13) C NMR.
26362834	4	71	theme	high	795:798	arg1	level					800:804	a high level	793:804	a high level of intraday and interday precision	793:839	NMR acquisition parameters and data processing have been optimized to reach a high level of intraday and interday precision (<0.2%).
26362834	3	72	theme	C6	626:627	arg1	positions					629:637	the C1, C2, C5, and the C1, C4, C5, C6 positions	590:637	the C1, C2, C5, and the C1, C4, C5, C6 positions	590:637	The method is based on calculating relative (13) C content of the C1, C2, C5, and the C1, C4, C5, C6 positions of the glycosyl and fructosyl moieties of the sucrose molecule, respectively.
26362834	0	73	theme	abundance	88:96	arg1	NMR					105:107	quantitative natural abundance (13) C NMR	67:107	quantitative natural abundance (13) C NMR	67:107	Authentication of the origin of sucrose-based sugar products using quantitative natural abundance (13) C NMR.
26362834	5	74	theme	beet	900:903	arg1	blends					922:927	the beet sugar-cane sugar blends	896:927	the beet sugar-cane sugar blends containing from 0 to 100 wt% of beet sugar	896:970	Good linearity (R(2) = 0.93) was obtained for the beet sugar-cane sugar blends containing from 0 to 100 wt% of beet sugar.
26362834	1	75	with	falsification	137:149	arg1	alternative					187:197	cheaper alternative	179:197	cheaper alternative (sugar beet)	179:210	BACKGROUND Due to possible falsification of sugar cane products with cheaper alternative (sugar beet) on the market, a simple analytical methodology needs to be developed to control the authenticity of sugar products.
26362834	1	75	with	falsification	137:149	arg1	beet					206:209	sugar beet	200:209	sugar beet	200:209	BACKGROUND Due to possible falsification of sugar cane products with cheaper alternative (sugar beet) on the market, a simple analytical methodology needs to be developed to control the authenticity of sugar products.
26362834	4	76	theme	data	748:751	arg1	processing					753:762	data processing	748:762	data processing	748:762	NMR acquisition parameters and data processing have been optimized to reach a high level of intraday and interday precision (<0.2%).
26362834	3	77	theme	fructosyl	659:667	arg1	moieties					669:676	the glycosyl and fructosyl moieties	642:676	moieties	669:676	The method is based on calculating relative (13) C content of the C1, C2, C5, and the C1, C4, C5, C6 positions of the glycosyl and fructosyl moieties of the sucrose molecule, respectively.
26362834	1	78	theme	sugar	312:316	arg1	products					318:325	sugar products	312:325	sugar products	312:325	BACKGROUND Due to possible falsification of sugar cane products with cheaper alternative (sugar beet) on the market, a simple analytical methodology needs to be developed to control the authenticity of sugar products.
26362834	3	79	theme	glycosyl	646:653	arg1	moieties					669:676	the glycosyl and fructosyl moieties	642:676	moieties	669:676	The method is based on calculating relative (13) C content of the C1, C2, C5, and the C1, C4, C5, C6 positions of the glycosyl and fructosyl moieties of the sucrose molecule, respectively.
26362834	0	80	theme	C	103:103	arg1	NMR					105:107	quantitative natural abundance (13) C NMR	67:107	quantitative natural abundance (13) C NMR	67:107	Authentication of the origin of sucrose-based sugar products using quantitative natural abundance (13) C NMR.
26362834	8	81	theme	complex	1287:1293	arg1	spectrometry					1314:1325	complex isotope ratio mass spectrometry	1287:1325	complex isotope ratio mass spectrometry measurements for routine detection and semi-quantification of adulteration of commercial cane sugar (C4 plant) with less expensive beet sugar (C3 plant)	1287:1478	CONCLUSION The (13) C NMR method is a good alternative to complex isotope ratio mass spectrometry measurements for routine detection and semi-quantification of adulteration of commercial cane sugar (C4 plant) with less expensive beet sugar (C3 plant).
26362834	2	82	dep	RESULTS	328:334	arg1	validated					401:409	validated	401:409	has been validated to differentiate between sucrose-based sugar products produced from sugar beet (C3 plant) and sugar cane (C4 plant)	392:525	RESULTS A direct (13) C nuclear magnetic resonance (NMR) method has been validated to differentiate between sucrose-based sugar products produced from sugar beet (C3 plant) and sugar cane (C4 plant).
26362834	8	83	theme	ratio	1303:1307	arg1	spectrometry					1314:1325	complex isotope ratio mass spectrometry	1287:1325	complex isotope ratio mass spectrometry measurements for routine detection and semi-quantification of adulteration of commercial cane sugar (C4 plant) with less expensive beet sugar (C3 plant)	1287:1478	CONCLUSION The (13) C NMR method is a good alternative to complex isotope ratio mass spectrometry measurements for routine detection and semi-quantification of adulteration of commercial cane sugar (C4 plant) with less expensive beet sugar (C3 plant).
26362834	2	84	theme	direct	338:343	arg1	NMR					380:382	NMR	380:382	NMR	380:382	RESULTS A direct (13) C nuclear magnetic resonance (NMR) method has been validated to differentiate between sucrose-based sugar products produced from sugar beet (C3 plant) and sugar cane (C4 plant).
26362834	2	84	theme	direct	338:343	arg1	resonance					369:377	direct (13) C nuclear magnetic resonance	338:377	A direct (13) C nuclear magnetic resonance (NMR) method	336:390	RESULTS A direct (13) C nuclear magnetic resonance (NMR) method has been validated to differentiate between sucrose-based sugar products produced from sugar beet (C3 plant) and sugar cane (C4 plant).
26362834	0	85	theme	origin	22:27	arg1	Authentication					0:13	Authentication	0:13	Authentication of the origin of sucrose-based sugar products	0:59	Authentication of the origin of sucrose-based sugar products using quantitative natural abundance (13) C NMR.
26362834	7	86	theme	Principal	1074:1082	arg1	PCA					1104:1106	PCA	1104:1106	PCA	1104:1106	Principal component analysis (PCA) was applied to the relative peak areas for replicate measurements to visualize the difference between studied products.
26362834	7	86	theme	Principal	1074:1082	arg1	analysis					1094:1101	Principal component analysis	1074:1101	Principal component analysis (PCA)	1074:1107	Principal component analysis (PCA) was applied to the relative peak areas for replicate measurements to visualize the difference between studied products.
26362834	8	87	theme	spectrometry	1314:1325	arg1	measurements					1327:1338	complex isotope ratio mass spectrometry measurements	1287:1338	complex isotope ratio mass spectrometry measurements for routine detection and semi-quantification of adulteration of commercial cane sugar (C4 plant) with less expensive beet sugar (C3 plant)	1287:1478	CONCLUSION The (13) C NMR method is a good alternative to complex isotope ratio mass spectrometry measurements for routine detection and semi-quantification of adulteration of commercial cane sugar (C4 plant) with less expensive beet sugar (C3 plant).
26362834	2	88	theme	magnetic	360:367	arg1	NMR					380:382	NMR	380:382	NMR	380:382	RESULTS A direct (13) C nuclear magnetic resonance (NMR) method has been validated to differentiate between sucrose-based sugar products produced from sugar beet (C3 plant) and sugar cane (C4 plant).
26362834	2	88	theme	magnetic	360:367	arg1	resonance					369:377	direct (13) C nuclear magnetic resonance	338:377	A direct (13) C nuclear magnetic resonance (NMR) method	336:390	RESULTS A direct (13) C nuclear magnetic resonance (NMR) method has been validated to differentiate between sucrose-based sugar products produced from sugar beet (C3 plant) and sugar cane (C4 plant).
26362834	0	89	theme	sucrose-based	32:44	arg1	products					52:59	sucrose-based sugar products	32:59	sucrose-based sugar products	32:59	Authentication of the origin of sucrose-based sugar products using quantitative natural abundance (13) C NMR.
26362834	3	90	dep	C1	594:595	arg1	the					590:592	the	590:592	the	590:592	The method is based on calculating relative (13) C content of the C1, C2, C5, and the C1, C4, C5, C6 positions of the glycosyl and fructosyl moieties of the sucrose molecule, respectively.
26362834	2	91	theme	C	350:350	arg1	NMR					380:382	NMR	380:382	NMR	380:382	RESULTS A direct (13) C nuclear magnetic resonance (NMR) method has been validated to differentiate between sucrose-based sugar products produced from sugar beet (C3 plant) and sugar cane (C4 plant).
26362834	2	91	theme	C	350:350	arg1	resonance					369:377	direct (13) C nuclear magnetic resonance	338:377	A direct (13) C nuclear magnetic resonance (NMR) method	336:390	RESULTS A direct (13) C nuclear magnetic resonance (NMR) method has been validated to differentiate between sucrose-based sugar products produced from sugar beet (C3 plant) and sugar cane (C4 plant).
26362834	0	92	theme	products	52:59	arg1	origin					22:27	the origin	18:27	the origin of sucrose-based sugar products	18:59	Authentication of the origin of sucrose-based sugar products using quantitative natural abundance (13) C NMR.
26362834	8	93	dep	CONCLUSION	1229:1238	arg1	alternative					1272:1282	a good alternative	1265:1282	a good alternative to complex isotope ratio mass spectrometry measurements for routine detection and semi-quantification of adulteration of commercial cane sugar (C4 plant) with less expensive beet sugar (C3 plant)	1265:1478	CONCLUSION The (13) C NMR method is a good alternative to complex isotope ratio mass spectrometry measurements for routine detection and semi-quantification of adulteration of commercial cane sugar (C4 plant) with less expensive beet sugar (C3 plant).
26362834	8	93	dep	CONCLUSION	1229:1238	arg1	method					1255:1260	The (13) C NMR method	1240:1260	The (13) C NMR method	1240:1260	CONCLUSION The (13) C NMR method is a good alternative to complex isotope ratio mass spectrometry measurements for routine detection and semi-quantification of adulteration of commercial cane sugar (C4 plant) with less expensive beet sugar (C3 plant).
26362834	8	94	theme	C4	1428:1429	arg1	plant					1431:1435	C4 plant	1428:1435	C4 plant	1428:1435	CONCLUSION The (13) C NMR method is a good alternative to complex isotope ratio mass spectrometry measurements for routine detection and semi-quantification of adulteration of commercial cane sugar (C4 plant) with less expensive beet sugar (C3 plant).
26362834	8	94	theme	C4	1428:1429	arg1	sugar					1421:1425	commercial cane sugar	1405:1425	commercial cane sugar (C4 plant)	1405:1436	CONCLUSION The (13) C NMR method is a good alternative to complex isotope ratio mass spectrometry measurements for routine detection and semi-quantification of adulteration of commercial cane sugar (C4 plant) with less expensive beet sugar (C3 plant).
26362834	9	95	theme	Chemical	1499:1506	arg1	Industry					1508:1515	Chemical Industry	1499:1515	Chemical Industry	1499:1515	© 2015 Society of Chemical Industry.
26362834	8	96	theme	adulteration	1389:1400	arg1	semi-quantification					1366:1384	semi-quantification	1366:1384	semi-quantification	1366:1384	CONCLUSION The (13) C NMR method is a good alternative to complex isotope ratio mass spectrometry measurements for routine detection and semi-quantification of adulteration of commercial cane sugar (C4 plant) with less expensive beet sugar (C3 plant).
26362834	8	96	theme	adulteration	1389:1400	arg1	detection					1352:1360	routine detection	1344:1360	routine detection	1344:1360	CONCLUSION The (13) C NMR method is a good alternative to complex isotope ratio mass spectrometry measurements for routine detection and semi-quantification of adulteration of commercial cane sugar (C4 plant) with less expensive beet sugar (C3 plant).
26793758	2	0	theme	chromatography	644:657	arg1	ionization					672:681	ion cyclotron resonance (FT-ICR) or high performance liquid chromatography electrospray ionization	584:681	ion cyclotron resonance (FT-ICR) or high performance liquid chromatography electrospray ionization (HPLC-ESI) linked to an Orbitrap	584:714	Specifically, glycan structure, sites, relative abundance levels, and termini structural conformation were investigated by use of Fourier transform ion cyclotron resonance (FT-ICR) or high performance liquid chromatography electrospray ionization (HPLC-ESI) linked to an Orbitrap.
26793758	2	0	theme	chromatography	644:657	arg1	HPLC-ESI					684:691	HPLC-ESI	684:691	HPLC-ESI	684:691	Specifically, glycan structure, sites, relative abundance levels, and termini structural conformation were investigated by use of Fourier transform ion cyclotron resonance (FT-ICR) or high performance liquid chromatography electrospray ionization (HPLC-ESI) linked to an Orbitrap.
26793758	1	1	dep	ionization	335:344	arg1	MALDI-ISD					363:371	MALDI-ISD	363:371	MALDI-ISD	363:371	A reference monoclonal antibody IgG1 and a fusion IgG protein were analyzed by top- and middle-down mass spectrometry with multiple fragmentation techniques including electron transfer dissociation (ETD) and matrix-assisted laser desorption ionization in-source decay (MALDI-ISD) to investigate heterogeneity of glycosylated protein species.
26793758	1	1	dep	ionization	335:344	arg1	decay					356:360	in-source decay	346:360	matrix-assisted laser desorption ionization in-source decay (MALDI-ISD)	302:372	A reference monoclonal antibody IgG1 and a fusion IgG protein were analyzed by top- and middle-down mass spectrometry with multiple fragmentation techniques including electron transfer dissociation (ETD) and matrix-assisted laser desorption ionization in-source decay (MALDI-ISD) to investigate heterogeneity of glycosylated protein species.
26793758	1	2	theme	transfer	270:277	arg1	dissociation					279:290	electron transfer dissociation	261:290	electron transfer dissociation (ETD)	261:296	A reference monoclonal antibody IgG1 and a fusion IgG protein were analyzed by top- and middle-down mass spectrometry with multiple fragmentation techniques including electron transfer dissociation (ETD) and matrix-assisted laser desorption ionization in-source decay (MALDI-ISD) to investigate heterogeneity of glycosylated protein species.
26793758	1	2	theme	transfer	270:277	arg1	ETD					293:295	ETD	293:295	ETD	293:295	A reference monoclonal antibody IgG1 and a fusion IgG protein were analyzed by top- and middle-down mass spectrometry with multiple fragmentation techniques including electron transfer dissociation (ETD) and matrix-assisted laser desorption ionization in-source decay (MALDI-ISD) to investigate heterogeneity of glycosylated protein species.
26793758	2	3	theme	structural	514:523	arg1	conformation					525:536	structural conformation	514:536	structural conformation	514:536	Specifically, glycan structure, sites, relative abundance levels, and termini structural conformation were investigated by use of Fourier transform ion cyclotron resonance (FT-ICR) or high performance liquid chromatography electrospray ionization (HPLC-ESI) linked to an Orbitrap.
26793758	3	4	theme	limited	733:739	arg1	digestion					751:759	Incorporating a limited enzymatic digestion	717:759	Incorporating a limited enzymatic digestion by immunoglobulin G-degrading enzyme Streptococcus pyogenes (IdeS) with MALDI-ISD analysis	717:850	Incorporating a limited enzymatic digestion by immunoglobulin G-degrading enzyme Streptococcus pyogenes (IdeS) with MALDI-ISD analysis extended sequence coverage of the internal region of the proteins without pre-fractionation.
26793758	2	5	theme	liquid	637:642	arg1	chromatography					644:657	high performance liquid chromatography	620:657	high performance liquid chromatography	620:657	Specifically, glycan structure, sites, relative abundance levels, and termini structural conformation were investigated by use of Fourier transform ion cyclotron resonance (FT-ICR) or high performance liquid chromatography electrospray ionization (HPLC-ESI) linked to an Orbitrap.
26793758	3	6	with	digestion	751:759	arg1	analysis					843:850	MALDI-ISD analysis	833:850	MALDI-ISD analysis	833:850	Incorporating a limited enzymatic digestion by immunoglobulin G-degrading enzyme Streptococcus pyogenes (IdeS) with MALDI-ISD analysis extended sequence coverage of the internal region of the proteins without pre-fractionation.
26793758	2	7	theme	Fourier	566:572	arg1	use					559:561	use	559:561	use of Fourier transform ion cyclotron resonance (FT-ICR) or high performance liquid chromatography electrospray ionization (HPLC-ESI) linked to an Orbitrap	559:714	Specifically, glycan structure, sites, relative abundance levels, and termini structural conformation were investigated by use of Fourier transform ion cyclotron resonance (FT-ICR) or high performance liquid chromatography electrospray ionization (HPLC-ESI) linked to an Orbitrap.
26793758	3	8	theme	enzymatic	741:749	arg1	digestion					751:759	Incorporating a limited enzymatic digestion	717:759	Incorporating a limited enzymatic digestion by immunoglobulin G-degrading enzyme Streptococcus pyogenes (IdeS) with MALDI-ISD analysis	717:850	Incorporating a limited enzymatic digestion by immunoglobulin G-degrading enzyme Streptococcus pyogenes (IdeS) with MALDI-ISD analysis extended sequence coverage of the internal region of the proteins without pre-fractionation.
26793758	2	9	theme	performance	625:635	arg1	chromatography					644:657	high performance liquid chromatography	620:657	high performance liquid chromatography	620:657	Specifically, glycan structure, sites, relative abundance levels, and termini structural conformation were investigated by use of Fourier transform ion cyclotron resonance (FT-ICR) or high performance liquid chromatography electrospray ionization (HPLC-ESI) linked to an Orbitrap.
26793758	3	10	theme	internal	886:893	arg1	region					895:900	the internal region	882:900	the internal region of the proteins	882:916	Incorporating a limited enzymatic digestion by immunoglobulin G-degrading enzyme Streptococcus pyogenes (IdeS) with MALDI-ISD analysis extended sequence coverage of the internal region of the proteins without pre-fractionation.
26793758	3	11	theme	enzyme	791:796	arg1	IdeS					822:825	IdeS	822:825	IdeS	822:825	Incorporating a limited enzymatic digestion by immunoglobulin G-degrading enzyme Streptococcus pyogenes (IdeS) with MALDI-ISD analysis extended sequence coverage of the internal region of the proteins without pre-fractionation.
26793758	3	11	theme	enzyme	791:796	arg1	pyogenes					812:819	immunoglobulin G-degrading enzyme Streptococcus pyogenes	764:819	immunoglobulin G-degrading enzyme Streptococcus pyogenes (IdeS)	764:826	Incorporating a limited enzymatic digestion by immunoglobulin G-degrading enzyme Streptococcus pyogenes (IdeS) with MALDI-ISD analysis extended sequence coverage of the internal region of the proteins without pre-fractionation.
26793758	2	12	theme	high	620:623	arg1	chromatography					644:657	high performance liquid chromatography	620:657	high performance liquid chromatography	620:657	Specifically, glycan structure, sites, relative abundance levels, and termini structural conformation were investigated by use of Fourier transform ion cyclotron resonance (FT-ICR) or high performance liquid chromatography electrospray ionization (HPLC-ESI) linked to an Orbitrap.
26793758	3	13	theme	G-degrading	779:789	arg1	IdeS					822:825	IdeS	822:825	IdeS	822:825	Incorporating a limited enzymatic digestion by immunoglobulin G-degrading enzyme Streptococcus pyogenes (IdeS) with MALDI-ISD analysis extended sequence coverage of the internal region of the proteins without pre-fractionation.
26793758	3	13	theme	G-degrading	779:789	arg1	pyogenes					812:819	immunoglobulin G-degrading enzyme Streptococcus pyogenes	764:819	immunoglobulin G-degrading enzyme Streptococcus pyogenes (IdeS)	764:826	Incorporating a limited enzymatic digestion by immunoglobulin G-degrading enzyme Streptococcus pyogenes (IdeS) with MALDI-ISD analysis extended sequence coverage of the internal region of the proteins without pre-fractionation.
26793758	3	14	theme	region	895:900	arg1	coverage					870:877	sequence coverage	861:877	sequence coverage of the internal region of the proteins	861:916	Incorporating a limited enzymatic digestion by immunoglobulin G-degrading enzyme Streptococcus pyogenes (IdeS) with MALDI-ISD analysis extended sequence coverage of the internal region of the proteins without pre-fractionation.
26793758	4	15	theme	Proteomics	1034:1043	arg1	Journal					1023:1029	Journal	1023:1029	Journal of Proteomics	1023:1043	The data in this article is associated with the research article published in Journal of Proteomics (Tran et al., 2015) [1].
26793758	3	16	theme	Streptococcus	798:810	arg1	IdeS					822:825	IdeS	822:825	IdeS	822:825	Incorporating a limited enzymatic digestion by immunoglobulin G-degrading enzyme Streptococcus pyogenes (IdeS) with MALDI-ISD analysis extended sequence coverage of the internal region of the proteins without pre-fractionation.
26793758	3	16	theme	Streptococcus	798:810	arg1	pyogenes					812:819	immunoglobulin G-degrading enzyme Streptococcus pyogenes	764:819	immunoglobulin G-degrading enzyme Streptococcus pyogenes (IdeS)	764:826	Incorporating a limited enzymatic digestion by immunoglobulin G-degrading enzyme Streptococcus pyogenes (IdeS) with MALDI-ISD analysis extended sequence coverage of the internal region of the proteins without pre-fractionation.
26793758	2	17	attach	linked	694:699	arg2	HPLC-ESI					684:691	HPLC-ESI	684:691	HPLC-ESI	684:691	Specifically, glycan structure, sites, relative abundance levels, and termini structural conformation were investigated by use of Fourier transform ion cyclotron resonance (FT-ICR) or high performance liquid chromatography electrospray ionization (HPLC-ESI) linked to an Orbitrap.
26793758	2	17	attach	linked	694:699	arg2	ionization					672:681	ion cyclotron resonance (FT-ICR) or high performance liquid chromatography electrospray ionization	584:681	ion cyclotron resonance (FT-ICR) or high performance liquid chromatography electrospray ionization (HPLC-ESI) linked to an Orbitrap	584:714	Specifically, glycan structure, sites, relative abundance levels, and termini structural conformation were investigated by use of Fourier transform ion cyclotron resonance (FT-ICR) or high performance liquid chromatography electrospray ionization (HPLC-ESI) linked to an Orbitrap.
26793758	2	17	attach	linked	694:699	arg1	Orbitrap					707:714	an Orbitrap	704:714	an Orbitrap	704:714	Specifically, glycan structure, sites, relative abundance levels, and termini structural conformation were investigated by use of Fourier transform ion cyclotron resonance (FT-ICR) or high performance liquid chromatography electrospray ionization (HPLC-ESI) linked to an Orbitrap.
26793758	3	18	theme	immunoglobulin	764:777	arg1	IdeS					822:825	IdeS	822:825	IdeS	822:825	Incorporating a limited enzymatic digestion by immunoglobulin G-degrading enzyme Streptococcus pyogenes (IdeS) with MALDI-ISD analysis extended sequence coverage of the internal region of the proteins without pre-fractionation.
26793758	3	18	theme	immunoglobulin	764:777	arg1	pyogenes					812:819	immunoglobulin G-degrading enzyme Streptococcus pyogenes	764:819	immunoglobulin G-degrading enzyme Streptococcus pyogenes (IdeS)	764:826	Incorporating a limited enzymatic digestion by immunoglobulin G-degrading enzyme Streptococcus pyogenes (IdeS) with MALDI-ISD analysis extended sequence coverage of the internal region of the proteins without pre-fractionation.
26793758	2	19	theme	relative	475:482	arg1	levels					494:499	relative abundance levels	475:499	relative abundance levels	475:499	Specifically, glycan structure, sites, relative abundance levels, and termini structural conformation were investigated by use of Fourier transform ion cyclotron resonance (FT-ICR) or high performance liquid chromatography electrospray ionization (HPLC-ESI) linked to an Orbitrap.
26793758	1	20	theme	top-	173:176	arg1	spectrometry					199:210	top- and middle-down mass spectrometry	173:210	top- and middle-down mass spectrometry with multiple fragmentation techniques including electron transfer dissociation (ETD) and matrix-assisted laser desorption ionization in-source decay (MALDI-ISD)	173:372	A reference monoclonal antibody IgG1 and a fusion IgG protein were analyzed by top- and middle-down mass spectrometry with multiple fragmentation techniques including electron transfer dissociation (ETD) and matrix-assisted laser desorption ionization in-source decay (MALDI-ISD) to investigate heterogeneity of glycosylated protein species.
26793758	2	21	theme	abundance	484:492	arg1	levels					494:499	relative abundance levels	475:499	relative abundance levels	475:499	Specifically, glycan structure, sites, relative abundance levels, and termini structural conformation were investigated by use of Fourier transform ion cyclotron resonance (FT-ICR) or high performance liquid chromatography electrospray ionization (HPLC-ESI) linked to an Orbitrap.
26793758	1	22	theme	glycosylated	406:417	arg1	species					427:433	glycosylated protein species	406:433	glycosylated protein species	406:433	A reference monoclonal antibody IgG1 and a fusion IgG protein were analyzed by top- and middle-down mass spectrometry with multiple fragmentation techniques including electron transfer dissociation (ETD) and matrix-assisted laser desorption ionization in-source decay (MALDI-ISD) to investigate heterogeneity of glycosylated protein species.
26793758	0	23	theme	Glycosylation	0:12	arg1	characterization					14:29	Glycosylation characterization	0:29	Glycosylation characterization of therapeutic mAbs by top- and middle-down mass spectrometry.	0:92	Glycosylation characterization of therapeutic mAbs by top- and middle-down mass spectrometry.
26793758	2	24	theme	electrospray	659:670	arg1	ionization					672:681	ion cyclotron resonance (FT-ICR) or high performance liquid chromatography electrospray ionization	584:681	ion cyclotron resonance (FT-ICR) or high performance liquid chromatography electrospray ionization (HPLC-ESI) linked to an Orbitrap	584:714	Specifically, glycan structure, sites, relative abundance levels, and termini structural conformation were investigated by use of Fourier transform ion cyclotron resonance (FT-ICR) or high performance liquid chromatography electrospray ionization (HPLC-ESI) linked to an Orbitrap.
26793758	2	24	theme	electrospray	659:670	arg1	HPLC-ESI					684:691	HPLC-ESI	684:691	HPLC-ESI	684:691	Specifically, glycan structure, sites, relative abundance levels, and termini structural conformation were investigated by use of Fourier transform ion cyclotron resonance (FT-ICR) or high performance liquid chromatography electrospray ionization (HPLC-ESI) linked to an Orbitrap.
26793758	1	25	theme	reference	96:104	arg1	antibody					117:124	A reference monoclonal antibody	94:124	A reference monoclonal antibody IgG1	94:129	A reference monoclonal antibody IgG1 and a fusion IgG protein were analyzed by top- and middle-down mass spectrometry with multiple fragmentation techniques including electron transfer dissociation (ETD) and matrix-assisted laser desorption ionization in-source decay (MALDI-ISD) to investigate heterogeneity of glycosylated protein species.
26793758	1	26	gly	heterogeneity	389:401	arg1	species					427:433	glycosylated protein species	406:433	glycosylated protein species	406:433	A reference monoclonal antibody IgG1 and a fusion IgG protein were analyzed by top- and middle-down mass spectrometry with multiple fragmentation techniques including electron transfer dissociation (ETD) and matrix-assisted laser desorption ionization in-source decay (MALDI-ISD) to investigate heterogeneity of glycosylated protein species.
26793758	1	27	theme	middle-down	182:192	arg1	spectrometry					199:210	top- and middle-down mass spectrometry	173:210	top- and middle-down mass spectrometry with multiple fragmentation techniques including electron transfer dissociation (ETD) and matrix-assisted laser desorption ionization in-source decay (MALDI-ISD)	173:372	A reference monoclonal antibody IgG1 and a fusion IgG protein were analyzed by top- and middle-down mass spectrometry with multiple fragmentation techniques including electron transfer dissociation (ETD) and matrix-assisted laser desorption ionization in-source decay (MALDI-ISD) to investigate heterogeneity of glycosylated protein species.
26793758	0	28	theme	therapeutic	34:44	arg1	mAbs					46:49	therapeutic mAbs	34:49	therapeutic mAbs	34:49	Glycosylation characterization of therapeutic mAbs by top- and middle-down mass spectrometry.
26793758	2	29	theme	resonance	598:606	arg1	ionization					672:681	ion cyclotron resonance (FT-ICR) or high performance liquid chromatography electrospray ionization	584:681	ion cyclotron resonance (FT-ICR) or high performance liquid chromatography electrospray ionization (HPLC-ESI) linked to an Orbitrap	584:714	Specifically, glycan structure, sites, relative abundance levels, and termini structural conformation were investigated by use of Fourier transform ion cyclotron resonance (FT-ICR) or high performance liquid chromatography electrospray ionization (HPLC-ESI) linked to an Orbitrap.
26793758	2	29	theme	resonance	598:606	arg1	HPLC-ESI					684:691	HPLC-ESI	684:691	HPLC-ESI	684:691	Specifically, glycan structure, sites, relative abundance levels, and termini structural conformation were investigated by use of Fourier transform ion cyclotron resonance (FT-ICR) or high performance liquid chromatography electrospray ionization (HPLC-ESI) linked to an Orbitrap.
26793758	1	30	theme	monoclonal	106:115	arg1	antibody					117:124	A reference monoclonal antibody	94:124	A reference monoclonal antibody IgG1	94:129	A reference monoclonal antibody IgG1 and a fusion IgG protein were analyzed by top- and middle-down mass spectrometry with multiple fragmentation techniques including electron transfer dissociation (ETD) and matrix-assisted laser desorption ionization in-source decay (MALDI-ISD) to investigate heterogeneity of glycosylated protein species.
26793758	1	31	theme	mass	194:197	arg1	spectrometry					199:210	top- and middle-down mass spectrometry	173:210	top- and middle-down mass spectrometry with multiple fragmentation techniques including electron transfer dissociation (ETD) and matrix-assisted laser desorption ionization in-source decay (MALDI-ISD)	173:372	A reference monoclonal antibody IgG1 and a fusion IgG protein were analyzed by top- and middle-down mass spectrometry with multiple fragmentation techniques including electron transfer dissociation (ETD) and matrix-assisted laser desorption ionization in-source decay (MALDI-ISD) to investigate heterogeneity of glycosylated protein species.
26793758	1	32	theme	matrix-assisted	302:316	arg1	ionization					335:344	matrix-assisted laser desorption ionization in-source decay (MALDI-ISD)	302:372	matrix-assisted laser desorption ionization in-source decay (MALDI-ISD)	302:372	A reference monoclonal antibody IgG1 and a fusion IgG protein were analyzed by top- and middle-down mass spectrometry with multiple fragmentation techniques including electron transfer dissociation (ETD) and matrix-assisted laser desorption ionization in-source decay (MALDI-ISD) to investigate heterogeneity of glycosylated protein species.
26793758	2	33	theme	cyclotron	588:596	arg1	FT-ICR					609:614	FT-ICR	609:614	FT-ICR	609:614	Specifically, glycan structure, sites, relative abundance levels, and termini structural conformation were investigated by use of Fourier transform ion cyclotron resonance (FT-ICR) or high performance liquid chromatography electrospray ionization (HPLC-ESI) linked to an Orbitrap.
26793758	2	33	theme	cyclotron	588:596	arg1	resonance					598:606	ion cyclotron resonance	584:606	ion cyclotron resonance (FT-ICR)	584:615	Specifically, glycan structure, sites, relative abundance levels, and termini structural conformation were investigated by use of Fourier transform ion cyclotron resonance (FT-ICR) or high performance liquid chromatography electrospray ionization (HPLC-ESI) linked to an Orbitrap.
26793758	1	34	theme	protein	419:425	arg1	species					427:433	glycosylated protein species	406:433	glycosylated protein species	406:433	A reference monoclonal antibody IgG1 and a fusion IgG protein were analyzed by top- and middle-down mass spectrometry with multiple fragmentation techniques including electron transfer dissociation (ETD) and matrix-assisted laser desorption ionization in-source decay (MALDI-ISD) to investigate heterogeneity of glycosylated protein species.
26793758	1	35	theme	antibody	117:124	arg1	IgG1					126:129	A reference monoclonal antibody IgG1	94:129	A reference monoclonal antibody IgG1	94:129	A reference monoclonal antibody IgG1 and a fusion IgG protein were analyzed by top- and middle-down mass spectrometry with multiple fragmentation techniques including electron transfer dissociation (ETD) and matrix-assisted laser desorption ionization in-source decay (MALDI-ISD) to investigate heterogeneity of glycosylated protein species.
26793758	1	36	gly	glycosylated	406:417	arg1	species					427:433	glycosylated protein species	406:433	glycosylated protein species	406:433	A reference monoclonal antibody IgG1 and a fusion IgG protein were analyzed by top- and middle-down mass spectrometry with multiple fragmentation techniques including electron transfer dissociation (ETD) and matrix-assisted laser desorption ionization in-source decay (MALDI-ISD) to investigate heterogeneity of glycosylated protein species.
26793758	1	37	theme	laser	318:322	arg1	ionization					335:344	matrix-assisted laser desorption ionization in-source decay (MALDI-ISD)	302:372	matrix-assisted laser desorption ionization in-source decay (MALDI-ISD)	302:372	A reference monoclonal antibody IgG1 and a fusion IgG protein were analyzed by top- and middle-down mass spectrometry with multiple fragmentation techniques including electron transfer dissociation (ETD) and matrix-assisted laser desorption ionization in-source decay (MALDI-ISD) to investigate heterogeneity of glycosylated protein species.
26793758	2	38	theme	ion	584:586	arg1	FT-ICR					609:614	FT-ICR	609:614	FT-ICR	609:614	Specifically, glycan structure, sites, relative abundance levels, and termini structural conformation were investigated by use of Fourier transform ion cyclotron resonance (FT-ICR) or high performance liquid chromatography electrospray ionization (HPLC-ESI) linked to an Orbitrap.
26793758	2	38	theme	ion	584:586	arg1	resonance					598:606	ion cyclotron resonance	584:606	ion cyclotron resonance (FT-ICR)	584:615	Specifically, glycan structure, sites, relative abundance levels, and termini structural conformation were investigated by use of Fourier transform ion cyclotron resonance (FT-ICR) or high performance liquid chromatography electrospray ionization (HPLC-ESI) linked to an Orbitrap.
26793758	1	39	theme	species	427:433	arg1	heterogeneity					389:401	heterogeneity	389:401	heterogeneity of glycosylated protein species	389:433	A reference monoclonal antibody IgG1 and a fusion IgG protein were analyzed by top- and middle-down mass spectrometry with multiple fragmentation techniques including electron transfer dissociation (ETD) and matrix-assisted laser desorption ionization in-source decay (MALDI-ISD) to investigate heterogeneity of glycosylated protein species.
26793758	2	40	theme	glycan	450:455	arg1	structure					457:465	glycan structure	450:465	glycan structure	450:465	Specifically, glycan structure, sites, relative abundance levels, and termini structural conformation were investigated by use of Fourier transform ion cyclotron resonance (FT-ICR) or high performance liquid chromatography electrospray ionization (HPLC-ESI) linked to an Orbitrap.
26793758	0	41	theme	mAbs	46:49	arg1	characterization					14:29	Glycosylation characterization	0:29	Glycosylation characterization of therapeutic mAbs by top- and middle-down mass spectrometry.	0:92	Glycosylation characterization of therapeutic mAbs by top- and middle-down mass spectrometry.
26793758	1	42	theme	desorption	324:333	arg1	ionization					335:344	matrix-assisted laser desorption ionization in-source decay (MALDI-ISD)	302:372	matrix-assisted laser desorption ionization in-source decay (MALDI-ISD)	302:372	A reference monoclonal antibody IgG1 and a fusion IgG protein were analyzed by top- and middle-down mass spectrometry with multiple fragmentation techniques including electron transfer dissociation (ETD) and matrix-assisted laser desorption ionization in-source decay (MALDI-ISD) to investigate heterogeneity of glycosylated protein species.
26793758	1	43	with	spectrometry	199:210	arg1	ionization					335:344	matrix-assisted laser desorption ionization in-source decay (MALDI-ISD)	302:372	matrix-assisted laser desorption ionization in-source decay (MALDI-ISD)	302:372	A reference monoclonal antibody IgG1 and a fusion IgG protein were analyzed by top- and middle-down mass spectrometry with multiple fragmentation techniques including electron transfer dissociation (ETD) and matrix-assisted laser desorption ionization in-source decay (MALDI-ISD) to investigate heterogeneity of glycosylated protein species.
26793758	1	43	with	spectrometry	199:210	arg1	techniques					240:249	multiple fragmentation techniques	217:249	multiple fragmentation techniques including electron transfer dissociation (ETD) and matrix-assisted laser desorption ionization in-source decay (MALDI-ISD)	217:372	A reference monoclonal antibody IgG1 and a fusion IgG protein were analyzed by top- and middle-down mass spectrometry with multiple fragmentation techniques including electron transfer dissociation (ETD) and matrix-assisted laser desorption ionization in-source decay (MALDI-ISD) to investigate heterogeneity of glycosylated protein species.
26793758	1	43	with	spectrometry	199:210	arg1	dissociation					279:290	electron transfer dissociation	261:290	electron transfer dissociation (ETD)	261:296	A reference monoclonal antibody IgG1 and a fusion IgG protein were analyzed by top- and middle-down mass spectrometry with multiple fragmentation techniques including electron transfer dissociation (ETD) and matrix-assisted laser desorption ionization in-source decay (MALDI-ISD) to investigate heterogeneity of glycosylated protein species.
26793758	1	44	theme	multiple	217:224	arg1	ionization					335:344	matrix-assisted laser desorption ionization in-source decay (MALDI-ISD)	302:372	matrix-assisted laser desorption ionization in-source decay (MALDI-ISD)	302:372	A reference monoclonal antibody IgG1 and a fusion IgG protein were analyzed by top- and middle-down mass spectrometry with multiple fragmentation techniques including electron transfer dissociation (ETD) and matrix-assisted laser desorption ionization in-source decay (MALDI-ISD) to investigate heterogeneity of glycosylated protein species.
26793758	1	44	theme	multiple	217:224	arg1	techniques					240:249	multiple fragmentation techniques	217:249	multiple fragmentation techniques including electron transfer dissociation (ETD) and matrix-assisted laser desorption ionization in-source decay (MALDI-ISD)	217:372	A reference monoclonal antibody IgG1 and a fusion IgG protein were analyzed by top- and middle-down mass spectrometry with multiple fragmentation techniques including electron transfer dissociation (ETD) and matrix-assisted laser desorption ionization in-source decay (MALDI-ISD) to investigate heterogeneity of glycosylated protein species.
26793758	1	44	theme	multiple	217:224	arg1	dissociation					279:290	electron transfer dissociation	261:290	electron transfer dissociation (ETD)	261:296	A reference monoclonal antibody IgG1 and a fusion IgG protein were analyzed by top- and middle-down mass spectrometry with multiple fragmentation techniques including electron transfer dissociation (ETD) and matrix-assisted laser desorption ionization in-source decay (MALDI-ISD) to investigate heterogeneity of glycosylated protein species.
26793758	2	45	dep	Fourier	566:572	arg1	transform					574:582	transform	574:582	transform ion cyclotron resonance (FT-ICR) or high performance liquid chromatography electrospray ionization (HPLC-ESI) linked to an Orbitrap	574:714	Specifically, glycan structure, sites, relative abundance levels, and termini structural conformation were investigated by use of Fourier transform ion cyclotron resonance (FT-ICR) or high performance liquid chromatography electrospray ionization (HPLC-ESI) linked to an Orbitrap.
26793758	4	46	dep	associated	973:982	arg1	Tran					1046:1049	Tran	1046:1049	Tran	1046:1049	The data in this article is associated with the research article published in Journal of Proteomics (Tran et al., 2015) [1].
26793758	4	47	from	data	949:952	arg1	article					962:968	this article	957:968	this article	957:968	The data in this article is associated with the research article published in Journal of Proteomics (Tran et al., 2015) [1].
26793758	0	48	theme	top-	54:57	arg1	spectrometry					80:91	top- and middle-down mass spectrometry	54:91	top- and middle-down mass spectrometry	54:91	Glycosylation characterization of therapeutic mAbs by top- and middle-down mass spectrometry.
26793758	1	49	theme	in-source	346:354	arg1	MALDI-ISD					363:371	MALDI-ISD	363:371	MALDI-ISD	363:371	A reference monoclonal antibody IgG1 and a fusion IgG protein were analyzed by top- and middle-down mass spectrometry with multiple fragmentation techniques including electron transfer dissociation (ETD) and matrix-assisted laser desorption ionization in-source decay (MALDI-ISD) to investigate heterogeneity of glycosylated protein species.
26793758	1	49	theme	in-source	346:354	arg1	decay					356:360	in-source decay	346:360	matrix-assisted laser desorption ionization in-source decay (MALDI-ISD)	302:372	A reference monoclonal antibody IgG1 and a fusion IgG protein were analyzed by top- and middle-down mass spectrometry with multiple fragmentation techniques including electron transfer dissociation (ETD) and matrix-assisted laser desorption ionization in-source decay (MALDI-ISD) to investigate heterogeneity of glycosylated protein species.
26793758	4	50	theme	research	993:1000	arg1	article					1002:1008	the research article	989:1008	the research article published in Journal of Proteomics	989:1043	The data in this article is associated with the research article published in Journal of Proteomics (Tran et al., 2015) [1].
26793758	3	51	theme	Incorporating	717:729	arg1	digestion					751:759	Incorporating a limited enzymatic digestion	717:759	Incorporating a limited enzymatic digestion by immunoglobulin G-degrading enzyme Streptococcus pyogenes (IdeS) with MALDI-ISD analysis	717:850	Incorporating a limited enzymatic digestion by immunoglobulin G-degrading enzyme Streptococcus pyogenes (IdeS) with MALDI-ISD analysis extended sequence coverage of the internal region of the proteins without pre-fractionation.
26793758	2	52	dep	structure	457:465	arg1	conformation					525:536	structural conformation	514:536	structural conformation	514:536	Specifically, glycan structure, sites, relative abundance levels, and termini structural conformation were investigated by use of Fourier transform ion cyclotron resonance (FT-ICR) or high performance liquid chromatography electrospray ionization (HPLC-ESI) linked to an Orbitrap.
26793758	3	53	theme	MALDI-ISD	833:841	arg1	analysis					843:850	MALDI-ISD analysis	833:850	MALDI-ISD analysis	833:850	Incorporating a limited enzymatic digestion by immunoglobulin G-degrading enzyme Streptococcus pyogenes (IdeS) with MALDI-ISD analysis extended sequence coverage of the internal region of the proteins without pre-fractionation.
26793758	4	54	dep	Tran	1046:1049	arg1	[1					1065:1066	[1	1065:1066	Tran et al., 2015) [1	1046:1066	The data in this article is associated with the research article published in Journal of Proteomics (Tran et al., 2015) [1].
26793758	1	55	theme	fragmentation	226:238	arg1	ionization					335:344	matrix-assisted laser desorption ionization in-source decay (MALDI-ISD)	302:372	matrix-assisted laser desorption ionization in-source decay (MALDI-ISD)	302:372	A reference monoclonal antibody IgG1 and a fusion IgG protein were analyzed by top- and middle-down mass spectrometry with multiple fragmentation techniques including electron transfer dissociation (ETD) and matrix-assisted laser desorption ionization in-source decay (MALDI-ISD) to investigate heterogeneity of glycosylated protein species.
26793758	1	55	theme	fragmentation	226:238	arg1	techniques					240:249	multiple fragmentation techniques	217:249	multiple fragmentation techniques including electron transfer dissociation (ETD) and matrix-assisted laser desorption ionization in-source decay (MALDI-ISD)	217:372	A reference monoclonal antibody IgG1 and a fusion IgG protein were analyzed by top- and middle-down mass spectrometry with multiple fragmentation techniques including electron transfer dissociation (ETD) and matrix-assisted laser desorption ionization in-source decay (MALDI-ISD) to investigate heterogeneity of glycosylated protein species.
26793758	1	55	theme	fragmentation	226:238	arg1	dissociation					279:290	electron transfer dissociation	261:290	electron transfer dissociation (ETD)	261:296	A reference monoclonal antibody IgG1 and a fusion IgG protein were analyzed by top- and middle-down mass spectrometry with multiple fragmentation techniques including electron transfer dissociation (ETD) and matrix-assisted laser desorption ionization in-source decay (MALDI-ISD) to investigate heterogeneity of glycosylated protein species.
26793758	1	56	theme	fusion	137:142	arg1	protein					148:154	a fusion IgG protein	135:154	a fusion IgG protein	135:154	A reference monoclonal antibody IgG1 and a fusion IgG protein were analyzed by top- and middle-down mass spectrometry with multiple fragmentation techniques including electron transfer dissociation (ETD) and matrix-assisted laser desorption ionization in-source decay (MALDI-ISD) to investigate heterogeneity of glycosylated protein species.
26793758	3	57	theme	proteins	909:916	arg1	region					895:900	the internal region	882:900	the internal region of the proteins	882:916	Incorporating a limited enzymatic digestion by immunoglobulin G-degrading enzyme Streptococcus pyogenes (IdeS) with MALDI-ISD analysis extended sequence coverage of the internal region of the proteins without pre-fractionation.
26793758	0	58	theme	mass	75:78	arg1	spectrometry					80:91	top- and middle-down mass spectrometry	54:91	top- and middle-down mass spectrometry	54:91	Glycosylation characterization of therapeutic mAbs by top- and middle-down mass spectrometry.
26793758	1	59	theme	IgG	144:146	arg1	protein					148:154	a fusion IgG protein	135:154	a fusion IgG protein	135:154	A reference monoclonal antibody IgG1 and a fusion IgG protein were analyzed by top- and middle-down mass spectrometry with multiple fragmentation techniques including electron transfer dissociation (ETD) and matrix-assisted laser desorption ionization in-source decay (MALDI-ISD) to investigate heterogeneity of glycosylated protein species.
26793758	3	60	theme	sequence	861:868	arg1	coverage					870:877	sequence coverage	861:877	sequence coverage of the internal region of the proteins	861:916	Incorporating a limited enzymatic digestion by immunoglobulin G-degrading enzyme Streptococcus pyogenes (IdeS) with MALDI-ISD analysis extended sequence coverage of the internal region of the proteins without pre-fractionation.
26793758	0	61	theme	middle-down	63:73	arg1	spectrometry					80:91	top- and middle-down mass spectrometry	54:91	top- and middle-down mass spectrometry	54:91	Glycosylation characterization of therapeutic mAbs by top- and middle-down mass spectrometry.
26793758	1	62	theme	electron	261:268	arg1	dissociation					279:290	electron transfer dissociation	261:290	electron transfer dissociation (ETD)	261:296	A reference monoclonal antibody IgG1 and a fusion IgG protein were analyzed by top- and middle-down mass spectrometry with multiple fragmentation techniques including electron transfer dissociation (ETD) and matrix-assisted laser desorption ionization in-source decay (MALDI-ISD) to investigate heterogeneity of glycosylated protein species.
26793758	1	62	theme	electron	261:268	arg1	ETD					293:295	ETD	293:295	ETD	293:295	A reference monoclonal antibody IgG1 and a fusion IgG protein were analyzed by top- and middle-down mass spectrometry with multiple fragmentation techniques including electron transfer dissociation (ETD) and matrix-assisted laser desorption ionization in-source decay (MALDI-ISD) to investigate heterogeneity of glycosylated protein species.
27177462	8	0	theme	significant	1144:1154	arg1	activities					1173:1182	significant immunomodulation activities	1144:1182	significant immunomodulation activities	1144:1182	All of them were found to possess significant immunomodulation activities, while varied effects of them on macrophage functions were observed.
27177462	4	1	from	differences	774:784	arg1	characteristics					799:813	structure characteristics	789:813	structure characteristics	789:813	Different rheological properties for CP-30 and CP-40 were found, indicating the differences in structure characteristics between CP-30 and CP-40.
27177462	6	2	theme	higher	1017:1022	arg1	content					1024:1030	higher content	1017:1030	higher content of xylose	1017:1040	Compared with CP-30, CP-40 had lower molecular weight and higher content of xylose.
27177462	0	3	theme	Cassia	91:96	arg1	obtusifolia					98:108	Cassia obtusifolia	91:108	Cassia obtusifolia	91:108	Fractionation, physicochemical property and immunological activity of polysaccharides from Cassia obtusifolia.
27177462	8	4	from	effects	1198:1204	arg1	functions					1228:1236	macrophage functions	1217:1236	macrophage functions	1217:1236	All of them were found to possess significant immunomodulation activities, while varied effects of them on macrophage functions were observed.
27177462	9	5	theme	present	1268:1274	arg1	study					1276:1280	the present study	1264:1280	the present study	1264:1280	The aim of the present study was to develop a simple and efficient method to purify cassia polysaccharides, and investigate their physicochemical properties and biological activities, which was meaningful for their potential use in food industry and folk medicine.
27177462	2	6	from	results	315:321	arg1	characteristics					336:350	structure characteristics	326:350	structure characteristics	326:350	Considerable amounts of water-soluble polysaccharides were found in the whole seeds, while conflicting results on structure characteristics have been reported, and few studies have been reported on physicochemical properties and immunomodulatory activities.
27177462	1	7	from	drink	164:168	arg1	Asia					173:176	Asia	173:176	Asia	173:176	The seeds of Cassia obtusifolia are widely used as a drink in Asia and an additive in food industry.
27177462	1	7	from	drink	164:168	arg1	additive					185:192	additive	185:192	additive	185:192	The seeds of Cassia obtusifolia are widely used as a drink in Asia and an additive in food industry.
27177462	1	7	from	drink	164:168	arg1	industry					202:209	food industry	197:209	food industry	197:209	The seeds of Cassia obtusifolia are widely used as a drink in Asia and an additive in food industry.
27177462	8	8	theme	immunomodulation	1156:1171	arg1	activities					1173:1182	significant immunomodulation activities	1144:1182	significant immunomodulation activities	1144:1182	All of them were found to possess significant immunomodulation activities, while varied effects of them on macrophage functions were observed.
27177462	9	9	theme	simple	1299:1304	arg1	method					1320:1325	a simple and efficient method	1297:1325	a simple and efficient method to purify cassia polysaccharides, and investigate their physicochemical properties and biological activities, which was meaningful for their potential use in food industry and folk medicine	1297:1515	The aim of the present study was to develop a simple and efficient method to purify cassia polysaccharides, and investigate their physicochemical properties and biological activities, which was meaningful for their potential use in food industry and folk medicine.
27177462	2	10	theme	whole	284:288	arg1	seeds					290:294	the whole seeds	280:294	the whole seeds	280:294	Considerable amounts of water-soluble polysaccharides were found in the whole seeds, while conflicting results on structure characteristics have been reported, and few studies have been reported on physicochemical properties and immunomodulatory activities.
27177462	6	11	theme	molecular	996:1004	arg1	weight					1006:1011	lower molecular weight	990:1011	lower molecular weight	990:1011	Compared with CP-30, CP-40 had lower molecular weight and higher content of xylose.
27177462	3	12	theme	ethanol	501:507	arg1	precipitation					509:521	gradient ethanol precipitation	492:521	gradient ethanol precipitation	492:521	In the present study, gradient ethanol precipitation was applied to fractionate the water-soluble polysaccharide (CP), and two sub-fractions CP-30 (30% ethanol precipitate) and CP-40 (40% ethanol precipitate) were obtained.
27177462	5	13	theme	Chemical	840:847	arg1	properties					849:858	Chemical properties	840:858	Chemical properties	840:858	Chemical properties, including molecular weight, monosaccharide composition, and glycosidic linkage were investigated.
27177462	5	13	theme	Chemical	840:847	arg1	linkage					932:938	glycosidic linkage	921:938	glycosidic linkage	921:938	Chemical properties, including molecular weight, monosaccharide composition, and glycosidic linkage were investigated.
27177462	5	13	theme	Chemical	840:847	arg1	weight					881:886	molecular weight	871:886	molecular weight	871:886	Chemical properties, including molecular weight, monosaccharide composition, and glycosidic linkage were investigated.
27177462	5	13	theme	Chemical	840:847	arg1	composition					904:914	monosaccharide composition	889:914	monosaccharide composition	889:914	Chemical properties, including molecular weight, monosaccharide composition, and glycosidic linkage were investigated.
27177462	9	14	theme	efficient	1310:1318	arg1	method					1320:1325	a simple and efficient method	1297:1325	a simple and efficient method to purify cassia polysaccharides, and investigate their physicochemical properties and biological activities, which was meaningful for their potential use in food industry and folk medicine	1297:1515	The aim of the present study was to develop a simple and efficient method to purify cassia polysaccharides, and investigate their physicochemical properties and biological activities, which was meaningful for their potential use in food industry and folk medicine.
27177462	8	15	theme	macrophage	1217:1226	arg1	functions					1228:1236	macrophage functions	1217:1236	macrophage functions	1217:1236	All of them were found to possess significant immunomodulation activities, while varied effects of them on macrophage functions were observed.
27177462	1	16	used	used	154:157	arg2	drink					164:168	a drink	162:168	a drink in Asia and an additive in food industry	162:209	The seeds of Cassia obtusifolia are widely used as a drink in Asia and an additive in food industry.
27177462	1	16	used	used	154:157	arg2	seeds					115:119	The seeds	111:119	The seeds of Cassia obtusifolia	111:141	The seeds of Cassia obtusifolia are widely used as a drink in Asia and an additive in food industry.
27177462	3	17	dep	sub-fractions	597:609	arg1	CP-30					611:615	CP-30	611:615	CP-30	611:615	In the present study, gradient ethanol precipitation was applied to fractionate the water-soluble polysaccharide (CP), and two sub-fractions CP-30 (30% ethanol precipitate) and CP-40 (40% ethanol precipitate) were obtained.
27177462	3	17	dep	sub-fractions	597:609	arg1	CP-40					647:651	CP-40	647:651	CP-40	647:651	In the present study, gradient ethanol precipitation was applied to fractionate the water-soluble polysaccharide (CP), and two sub-fractions CP-30 (30% ethanol precipitate) and CP-40 (40% ethanol precipitate) were obtained.
27177462	3	17	dep	sub-fractions	597:609	arg1	sub-fractions					597:609	two sub-fractions	593:609	two sub-fractions CP-30 (30% ethanol precipitate) and CP-40 (40% ethanol precipitate)	593:677	In the present study, gradient ethanol precipitation was applied to fractionate the water-soluble polysaccharide (CP), and two sub-fractions CP-30 (30% ethanol precipitate) and CP-40 (40% ethanol precipitate) were obtained.
27177462	3	17	dep	sub-fractions	597:609	arg1	precipitate					630:640	30% ethanol precipitate	618:640	30% ethanol precipitate	618:640	In the present study, gradient ethanol precipitation was applied to fractionate the water-soluble polysaccharide (CP), and two sub-fractions CP-30 (30% ethanol precipitate) and CP-40 (40% ethanol precipitate) were obtained.
27177462	7	18	theme	CP-40	1089:1093	arg1	effects					1064:1070	The immunomodulatory effects	1043:1070	The immunomodulatory effects of CP, CP-30 and CP-40	1043:1093	The immunomodulatory effects of CP, CP-30 and CP-40 were assessed.
27177462	8	19	contain	possess	1136:1142	arg2	activities					1173:1182	significant immunomodulation activities	1144:1182	significant immunomodulation activities	1144:1182	All of them were found to possess significant immunomodulation activities, while varied effects of them on macrophage functions were observed.
27177462	8	19	contain	possess	1136:1142	arg1	them					1117:1120	them	1117:1120	them	1117:1120	All of them were found to possess significant immunomodulation activities, while varied effects of them on macrophage functions were observed.
27177462	8	19	contain	possess	1136:1142	arg1	All					1110:1112	All	1110:1112	All	1110:1112	All of them were found to possess significant immunomodulation activities, while varied effects of them on macrophage functions were observed.
27177462	9	20	theme	folk	1503:1506	arg1	medicine					1508:1515	folk medicine	1503:1515	folk medicine	1503:1515	The aim of the present study was to develop a simple and efficient method to purify cassia polysaccharides, and investigate their physicochemical properties and biological activities, which was meaningful for their potential use in food industry and folk medicine.
27177462	8	21	theme	varied	1191:1196	arg1	effects					1198:1204	varied effects	1191:1204	varied effects of them on macrophage functions	1191:1236	All of them were found to possess significant immunomodulation activities, while varied effects of them on macrophage functions were observed.
27177462	6	22	theme	lower	990:994	arg1	weight					1006:1011	lower molecular weight	990:1011	lower molecular weight	990:1011	Compared with CP-30, CP-40 had lower molecular weight and higher content of xylose.
27177462	2	23	theme	conflicting	303:313	arg1	results					315:321	conflicting results	303:321	conflicting results on structure characteristics	303:350	Considerable amounts of water-soluble polysaccharides were found in the whole seeds, while conflicting results on structure characteristics have been reported, and few studies have been reported on physicochemical properties and immunomodulatory activities.
27177462	2	24	theme	few	376:378	arg1	studies					380:386	few studies	376:386	few studies	376:386	Considerable amounts of water-soluble polysaccharides were found in the whole seeds, while conflicting results on structure characteristics have been reported, and few studies have been reported on physicochemical properties and immunomodulatory activities.
27177462	2	25	theme	immunomodulatory	441:456	arg1	activities					458:467	immunomodulatory activities	441:467	immunomodulatory activities	441:467	Considerable amounts of water-soluble polysaccharides were found in the whole seeds, while conflicting results on structure characteristics have been reported, and few studies have been reported on physicochemical properties and immunomodulatory activities.
27177462	7	26	theme	CP-30	1079:1083	arg1	effects					1064:1070	The immunomodulatory effects	1043:1070	The immunomodulatory effects of CP, CP-30 and CP-40	1043:1093	The immunomodulatory effects of CP, CP-30 and CP-40 were assessed.
27177462	3	27	theme	present	477:483	arg1	study					485:489	the present study	473:489	the present study	473:489	In the present study, gradient ethanol precipitation was applied to fractionate the water-soluble polysaccharide (CP), and two sub-fractions CP-30 (30% ethanol precipitate) and CP-40 (40% ethanol precipitate) were obtained.
27177462	0	28	theme	physicochemical	15:29	arg1	property					31:38	physicochemical property	15:38	physicochemical property	15:38	Fractionation, physicochemical property and immunological activity of polysaccharides from Cassia obtusifolia.
27177462	9	29	theme	cassia	1337:1342	arg1	polysaccharides					1344:1358	cassia polysaccharides	1337:1358	cassia polysaccharides	1337:1358	The aim of the present study was to develop a simple and efficient method to purify cassia polysaccharides, and investigate their physicochemical properties and biological activities, which was meaningful for their potential use in food industry and folk medicine.
27177462	0	30	from	obtusifolia	98:108	arg1	Fractionation					0:12	Fractionation	0:12	Fractionation	0:12	Fractionation, physicochemical property and immunological activity of polysaccharides from Cassia obtusifolia.
27177462	0	30	from	obtusifolia	98:108	arg1	property					31:38	physicochemical property	15:38	physicochemical property	15:38	Fractionation, physicochemical property and immunological activity of polysaccharides from Cassia obtusifolia.
27177462	0	30	from	obtusifolia	98:108	arg1	activity					58:65	immunological activity	44:65	immunological activity	44:65	Fractionation, physicochemical property and immunological activity of polysaccharides from Cassia obtusifolia.
27177462	7	31	theme	immunomodulatory	1047:1062	arg1	effects					1064:1070	The immunomodulatory effects	1043:1070	The immunomodulatory effects of CP, CP-30 and CP-40	1043:1093	The immunomodulatory effects of CP, CP-30 and CP-40 were assessed.
27177462	2	32	theme	physicochemical	410:424	arg1	properties					426:435	physicochemical properties	410:435	physicochemical properties	410:435	Considerable amounts of water-soluble polysaccharides were found in the whole seeds, while conflicting results on structure characteristics have been reported, and few studies have been reported on physicochemical properties and immunomodulatory activities.
27177462	0	33	theme	immunological	44:56	arg1	activity					58:65	immunological activity	44:65	immunological activity	44:65	Fractionation, physicochemical property and immunological activity of polysaccharides from Cassia obtusifolia.
27177462	4	34	theme	Different	694:702	arg1	properties					716:725	Different rheological properties	694:725	Different rheological properties for CP-30 and CP-40	694:745	Different rheological properties for CP-30 and CP-40 were found, indicating the differences in structure characteristics between CP-30 and CP-40.
27177462	7	35	theme	CP	1075:1076	arg1	effects					1064:1070	The immunomodulatory effects	1043:1070	The immunomodulatory effects of CP, CP-30 and CP-40	1043:1093	The immunomodulatory effects of CP, CP-30 and CP-40 were assessed.
27177462	9	36	theme	food	1485:1488	arg1	industry					1490:1497	food industry	1485:1497	food industry	1485:1497	The aim of the present study was to develop a simple and efficient method to purify cassia polysaccharides, and investigate their physicochemical properties and biological activities, which was meaningful for their potential use in food industry and folk medicine.
27177462	4	37	theme	structure	789:797	arg1	characteristics					799:813	structure characteristics	789:813	structure characteristics	789:813	Different rheological properties for CP-30 and CP-40 were found, indicating the differences in structure characteristics between CP-30 and CP-40.
27177462	9	38	theme	potential	1468:1476	arg1	use					1478:1480	their potential use	1462:1480	their potential use in food industry and folk medicine	1462:1515	The aim of the present study was to develop a simple and efficient method to purify cassia polysaccharides, and investigate their physicochemical properties and biological activities, which was meaningful for their potential use in food industry and folk medicine.
27177462	1	39	theme	food	197:200	arg1	industry					202:209	food industry	197:209	food industry	197:209	The seeds of Cassia obtusifolia are widely used as a drink in Asia and an additive in food industry.
27177462	8	40	theme	them	1209:1212	arg1	effects					1198:1204	varied effects	1191:1204	varied effects of them on macrophage functions	1191:1236	All of them were found to possess significant immunomodulation activities, while varied effects of them on macrophage functions were observed.
27177462	2	41	theme	Considerable	212:223	arg1	amounts					225:231	Considerable amounts	212:231	Considerable amounts of water-soluble polysaccharides	212:264	Considerable amounts of water-soluble polysaccharides were found in the whole seeds, while conflicting results on structure characteristics have been reported, and few studies have been reported on physicochemical properties and immunomodulatory activities.
27177462	2	41	theme	Considerable	212:223	arg1	polysaccharides					250:264	water-soluble polysaccharides	236:264	water-soluble polysaccharides	236:264	Considerable amounts of water-soluble polysaccharides were found in the whole seeds, while conflicting results on structure characteristics have been reported, and few studies have been reported on physicochemical properties and immunomodulatory activities.
27177462	1	42	theme	Cassia	124:129	arg1	obtusifolia					131:141	Cassia obtusifolia	124:141	Cassia obtusifolia	124:141	The seeds of Cassia obtusifolia are widely used as a drink in Asia and an additive in food industry.
27177462	6	43	contain	had	986:988	arg1	CP-40					980:984	CP-40	980:984	CP-40	980:984	Compared with CP-30, CP-40 had lower molecular weight and higher content of xylose.
27177462	6	43	contain	had	986:988	arg2	content					1024:1030	higher content	1017:1030	higher content of xylose	1017:1040	Compared with CP-30, CP-40 had lower molecular weight and higher content of xylose.
27177462	6	43	contain	had	986:988	arg2	weight					1006:1011	lower molecular weight	990:1011	lower molecular weight	990:1011	Compared with CP-30, CP-40 had lower molecular weight and higher content of xylose.
27177462	9	44	from	use	1478:1480	arg1	medicine					1508:1515	folk medicine	1503:1515	folk medicine	1503:1515	The aim of the present study was to develop a simple and efficient method to purify cassia polysaccharides, and investigate their physicochemical properties and biological activities, which was meaningful for their potential use in food industry and folk medicine.
27177462	9	44	from	use	1478:1480	arg1	industry					1490:1497	food industry	1485:1497	food industry	1485:1497	The aim of the present study was to develop a simple and efficient method to purify cassia polysaccharides, and investigate their physicochemical properties and biological activities, which was meaningful for their potential use in food industry and folk medicine.
27177462	1	45	theme	obtusifolia	131:141	arg1	seeds					115:119	The seeds	111:119	The seeds of Cassia obtusifolia	111:141	The seeds of Cassia obtusifolia are widely used as a drink in Asia and an additive in food industry.
27177462	1	45	theme	obtusifolia	131:141	arg1	drink					164:168	a drink	162:168	a drink in Asia and an additive in food industry	162:209	The seeds of Cassia obtusifolia are widely used as a drink in Asia and an additive in food industry.
27177462	2	46	theme	water-soluble	236:248	arg1	polysaccharides					250:264	water-soluble polysaccharides	236:264	water-soluble polysaccharides	236:264	Considerable amounts of water-soluble polysaccharides were found in the whole seeds, while conflicting results on structure characteristics have been reported, and few studies have been reported on physicochemical properties and immunomodulatory activities.
27177462	4	47	theme	rheological	704:714	arg1	properties					716:725	Different rheological properties	694:725	Different rheological properties for CP-30 and CP-40	694:745	Different rheological properties for CP-30 and CP-40 were found, indicating the differences in structure characteristics between CP-30 and CP-40.
27177462	3	48	theme	precipitate	666:676	arg1	%					656:656	40%	654:656	40% ethanol precipitate	654:676	In the present study, gradient ethanol precipitation was applied to fractionate the water-soluble polysaccharide (CP), and two sub-fractions CP-30 (30% ethanol precipitate) and CP-40 (40% ethanol precipitate) were obtained.
27177462	3	49	theme	ethanol	622:628	arg1	CP-30					611:615	CP-30	611:615	CP-30	611:615	In the present study, gradient ethanol precipitation was applied to fractionate the water-soluble polysaccharide (CP), and two sub-fractions CP-30 (30% ethanol precipitate) and CP-40 (40% ethanol precipitate) were obtained.
27177462	3	49	theme	ethanol	622:628	arg1	precipitate					630:640	30% ethanol precipitate	618:640	30% ethanol precipitate	618:640	In the present study, gradient ethanol precipitation was applied to fractionate the water-soluble polysaccharide (CP), and two sub-fractions CP-30 (30% ethanol precipitate) and CP-40 (40% ethanol precipitate) were obtained.
27177462	6	50	theme	xylose	1035:1040	arg1	weight					1006:1011	lower molecular weight	990:1011	lower molecular weight	990:1011	Compared with CP-30, CP-40 had lower molecular weight and higher content of xylose.
27177462	6	50	theme	xylose	1035:1040	arg1	content					1024:1030	higher content	1017:1030	higher content of xylose	1017:1040	Compared with CP-30, CP-40 had lower molecular weight and higher content of xylose.
27177462	2	51	theme	structure	326:334	arg1	characteristics					336:350	structure characteristics	326:350	structure characteristics	326:350	Considerable amounts of water-soluble polysaccharides were found in the whole seeds, while conflicting results on structure characteristics have been reported, and few studies have been reported on physicochemical properties and immunomodulatory activities.
27177462	9	52	theme	physicochemical	1383:1397	arg1	properties					1399:1408	their physicochemical properties	1377:1408	their physicochemical properties	1377:1408	The aim of the present study was to develop a simple and efficient method to purify cassia polysaccharides, and investigate their physicochemical properties and biological activities, which was meaningful for their potential use in food industry and folk medicine.
27177462	3	53	theme	water-soluble	554:566	arg1	polysaccharide					568:581	the water-soluble polysaccharide	550:581	the water-soluble polysaccharide (CP)	550:586	In the present study, gradient ethanol precipitation was applied to fractionate the water-soluble polysaccharide (CP), and two sub-fractions CP-30 (30% ethanol precipitate) and CP-40 (40% ethanol precipitate) were obtained.
27177462	3	53	theme	water-soluble	554:566	arg1	CP					584:585	CP	584:585	CP	584:585	In the present study, gradient ethanol precipitation was applied to fractionate the water-soluble polysaccharide (CP), and two sub-fractions CP-30 (30% ethanol precipitate) and CP-40 (40% ethanol precipitate) were obtained.
27177462	5	54	theme	glycosidic	921:930	arg1	linkage					932:938	glycosidic linkage	921:938	glycosidic linkage	921:938	Chemical properties, including molecular weight, monosaccharide composition, and glycosidic linkage were investigated.
27177462	3	55	theme	gradient	492:499	arg1	precipitation					509:521	gradient ethanol precipitation	492:521	gradient ethanol precipitation	492:521	In the present study, gradient ethanol precipitation was applied to fractionate the water-soluble polysaccharide (CP), and two sub-fractions CP-30 (30% ethanol precipitate) and CP-40 (40% ethanol precipitate) were obtained.
27177462	2	56	located	found	271:275	arg2	polysaccharides					250:264	water-soluble polysaccharides	236:264	water-soluble polysaccharides	236:264	Considerable amounts of water-soluble polysaccharides were found in the whole seeds, while conflicting results on structure characteristics have been reported, and few studies have been reported on physicochemical properties and immunomodulatory activities.
27177462	2	56	located	found	271:275	arg1	seeds					290:294	the whole seeds	280:294	the whole seeds	280:294	Considerable amounts of water-soluble polysaccharides were found in the whole seeds, while conflicting results on structure characteristics have been reported, and few studies have been reported on physicochemical properties and immunomodulatory activities.
27177462	2	56	located	found	271:275	arg2	amounts					225:231	Considerable amounts	212:231	Considerable amounts of water-soluble polysaccharides	212:264	Considerable amounts of water-soluble polysaccharides were found in the whole seeds, while conflicting results on structure characteristics have been reported, and few studies have been reported on physicochemical properties and immunomodulatory activities.
27177462	9	57	theme	study	1276:1280	arg1	aim					1257:1259	The aim	1253:1259	The aim of the present study	1253:1280	The aim of the present study was to develop a simple and efficient method to purify cassia polysaccharides, and investigate their physicochemical properties and biological activities, which was meaningful for their potential use in food industry and folk medicine.
27177462	2	58	theme	polysaccharides	250:264	arg1	amounts					225:231	Considerable amounts	212:231	Considerable amounts of water-soluble polysaccharides	212:264	Considerable amounts of water-soluble polysaccharides were found in the whole seeds, while conflicting results on structure characteristics have been reported, and few studies have been reported on physicochemical properties and immunomodulatory activities.
27177462	2	58	theme	polysaccharides	250:264	arg1	polysaccharides					250:264	water-soluble polysaccharides	236:264	water-soluble polysaccharides	236:264	Considerable amounts of water-soluble polysaccharides were found in the whole seeds, while conflicting results on structure characteristics have been reported, and few studies have been reported on physicochemical properties and immunomodulatory activities.
27177462	5	59	theme	monosaccharide	889:902	arg1	composition					904:914	monosaccharide composition	889:914	monosaccharide composition	889:914	Chemical properties, including molecular weight, monosaccharide composition, and glycosidic linkage were investigated.
27177462	5	60	theme	molecular	871:879	arg1	weight					881:886	molecular weight	871:886	molecular weight	871:886	Chemical properties, including molecular weight, monosaccharide composition, and glycosidic linkage were investigated.
27177462	3	61	dep	CP-40	647:651	arg1	%					656:656	40%	654:656	40% ethanol precipitate	654:676	In the present study, gradient ethanol precipitation was applied to fractionate the water-soluble polysaccharide (CP), and two sub-fractions CP-30 (30% ethanol precipitate) and CP-40 (40% ethanol precipitate) were obtained.
27177462	0	62	theme	polysaccharides	70:84	arg1	Fractionation					0:12	Fractionation	0:12	Fractionation	0:12	Fractionation, physicochemical property and immunological activity of polysaccharides from Cassia obtusifolia.
27177462	0	62	theme	polysaccharides	70:84	arg1	property					31:38	physicochemical property	15:38	physicochemical property	15:38	Fractionation, physicochemical property and immunological activity of polysaccharides from Cassia obtusifolia.
27177462	0	62	theme	polysaccharides	70:84	arg1	activity					58:65	immunological activity	44:65	immunological activity	44:65	Fractionation, physicochemical property and immunological activity of polysaccharides from Cassia obtusifolia.
27177462	3	63	theme	%	620:620	arg1	CP-30					611:615	CP-30	611:615	CP-30	611:615	In the present study, gradient ethanol precipitation was applied to fractionate the water-soluble polysaccharide (CP), and two sub-fractions CP-30 (30% ethanol precipitate) and CP-40 (40% ethanol precipitate) were obtained.
27177462	3	63	theme	%	620:620	arg1	precipitate					630:640	30% ethanol precipitate	618:640	30% ethanol precipitate	618:640	In the present study, gradient ethanol precipitation was applied to fractionate the water-soluble polysaccharide (CP), and two sub-fractions CP-30 (30% ethanol precipitate) and CP-40 (40% ethanol precipitate) were obtained.
27177462	9	64	theme	biological	1414:1423	arg1	activities					1425:1434	biological activities	1414:1434	biological activities	1414:1434	The aim of the present study was to develop a simple and efficient method to purify cassia polysaccharides, and investigate their physicochemical properties and biological activities, which was meaningful for their potential use in food industry and folk medicine.
28068519	0	0	theme	molecular	78:86	arg1	heparins					95:102	low molecular weight heparins	74:102	low molecular weight heparins	74:102	Qualification of HSQC methods for quantitative composition of heparin and low molecular weight heparins.
28068519	6	1	theme	detection	923:931	arg1	limits					913:918	limits	913:918	limits	913:918	A standard validation study on heparin then examined linearity, repeatability, intermediate precision and limits of detection and quantitation; selected validation parameters were also determined for LMWH.
28068519	6	1	theme	detection	923:931	arg1	repeatability					871:883	repeatability	871:883	repeatability	871:883	A standard validation study on heparin then examined linearity, repeatability, intermediate precision and limits of detection and quantitation; selected validation parameters were also determined for LMWH.
28068519	6	1	theme	detection	923:931	arg1	precision					899:907	intermediate precision	886:907	intermediate precision	886:907	A standard validation study on heparin then examined linearity, repeatability, intermediate precision and limits of detection and quantitation; selected validation parameters were also determined for LMWH.
28068519	6	1	theme	detection	923:931	arg1	linearity					860:868	linearity	860:868	linearity	860:868	A standard validation study on heparin then examined linearity, repeatability, intermediate precision and limits of detection and quantitation; selected validation parameters were also determined for LMWH.
28068519	3	2	theme	parameters	499:508	arg1	effects					467:473	the effects	463:473	the effects of several experimental parameters	463:508	Pre-validation work investigated the effects of several experimental parameters to assess robustness and to optimize critical factors.
28068519	1	3	theme	low	240:242	arg1	LMWH					271:274	LMWH	271:274	LMWH	271:274	An NMR HSQC method has recently been proposed for the quantitative determination of the mono- and disaccharide subunits of heparin and low molecular weight heparins (LMWH).
28068519	1	3	theme	low	240:242	arg1	heparins					261:268	low molecular weight heparins	240:268	low molecular weight heparins (LMWH)	240:275	An NMR HSQC method has recently been proposed for the quantitative determination of the mono- and disaccharide subunits of heparin and low molecular weight heparins (LMWH).
28068519	0	4	theme	low	74:76	arg1	heparins					95:102	low molecular weight heparins	74:102	low molecular weight heparins	74:102	Qualification of HSQC methods for quantitative composition of heparin and low molecular weight heparins.
28068519	6	5	theme	validation	960:969	arg1	parameters					971:980	selected validation parameters	951:980	selected validation parameters	951:980	A standard validation study on heparin then examined linearity, repeatability, intermediate precision and limits of detection and quantitation; selected validation parameters were also determined for LMWH.
28068519	1	6	theme	molecular	244:252	arg1	LMWH					271:274	LMWH	271:274	LMWH	271:274	An NMR HSQC method has recently been proposed for the quantitative determination of the mono- and disaccharide subunits of heparin and low molecular weight heparins (LMWH).
28068519	1	6	theme	molecular	244:252	arg1	heparins					261:268	low molecular weight heparins	240:268	low molecular weight heparins (LMWH)	240:275	An NMR HSQC method has recently been proposed for the quantitative determination of the mono- and disaccharide subunits of heparin and low molecular weight heparins (LMWH).
28068519	0	7	theme	heparins	95:102	arg1	composition					47:57	quantitative composition	34:57	quantitative composition of heparin and low molecular weight heparins	34:102	Qualification of HSQC methods for quantitative composition of heparin and low molecular weight heparins.
28068519	6	8	theme	selected	951:958	arg1	parameters					971:980	selected validation parameters	951:980	selected validation parameters	951:980	A standard validation study on heparin then examined linearity, repeatability, intermediate precision and limits of detection and quantitation; selected validation parameters were also determined for LMWH.
28068519	1	9	theme	weight	254:259	arg1	LMWH					271:274	LMWH	271:274	LMWH	271:274	An NMR HSQC method has recently been proposed for the quantitative determination of the mono- and disaccharide subunits of heparin and low molecular weight heparins (LMWH).
28068519	1	9	theme	weight	254:259	arg1	heparins					261:268	low molecular weight heparins	240:268	low molecular weight heparins (LMWH)	240:275	An NMR HSQC method has recently been proposed for the quantitative determination of the mono- and disaccharide subunits of heparin and low molecular weight heparins (LMWH).
28068519	0	10	theme	weight	88:93	arg1	heparins					95:102	low molecular weight heparins	74:102	low molecular weight heparins	74:102	Qualification of HSQC methods for quantitative composition of heparin and low molecular weight heparins.
28068519	3	11	theme	critical	547:554	arg1	factors					556:562	critical factors	547:562	critical factors	547:562	Pre-validation work investigated the effects of several experimental parameters to assess robustness and to optimize critical factors.
28068519	1	12	theme	heparins	261:268	arg1	subunits					216:223	the mono- and disaccharide subunits	189:223	the mono- and disaccharide subunits of heparin and low molecular weight heparins (LMWH)	189:275	An NMR HSQC method has recently been proposed for the quantitative determination of the mono- and disaccharide subunits of heparin and low molecular weight heparins (LMWH).
28068519	3	13	theme	several	478:484	arg1	parameters					499:508	several experimental parameters	478:508	several experimental parameters	478:508	Pre-validation work investigated the effects of several experimental parameters to assess robustness and to optimize critical factors.
28068519	2	14	theme	2D-NMR	358:363	arg1	method					365:370	the 2D-NMR method	354:370	the 2D-NMR method suitable for pharmaceutical quality control applications	354:427	The focus of the current study was the validation of this procedure to make the 2D-NMR method suitable for pharmaceutical quality control applications.
28068519	4	15	theme	pulse	604:608	arg1	selection					619:627	pulse sequence selection	604:627	pulse sequence selection	604:627	Important experimental parameters were pulse sequence selection, equilibration interval between pulse trains and temperature.
28068519	4	15	theme	pulse	604:608	arg1	interval					644:651	equilibration interval	630:651	equilibration interval between pulse trains and temperature	630:688	Important experimental parameters were pulse sequence selection, equilibration interval between pulse trains and temperature.
28068519	1	16	theme	quantitative	159:170	arg1	determination					172:184	the quantitative determination	155:184	the quantitative determination of the mono- and disaccharide subunits of heparin and low molecular weight heparins (LMWH)	155:275	An NMR HSQC method has recently been proposed for the quantitative determination of the mono- and disaccharide subunits of heparin and low molecular weight heparins (LMWH).
28068519	4	17	theme	Important	565:573	arg1	parameters					588:597	Important experimental parameters	565:597	Important experimental parameters	565:597	Important experimental parameters were pulse sequence selection, equilibration interval between pulse trains and temperature.
28068519	6	18	theme	quantitation	937:948	arg1	limits					913:918	limits	913:918	limits	913:918	A standard validation study on heparin then examined linearity, repeatability, intermediate precision and limits of detection and quantitation; selected validation parameters were also determined for LMWH.
28068519	6	18	theme	quantitation	937:948	arg1	repeatability					871:883	repeatability	871:883	repeatability	871:883	A standard validation study on heparin then examined linearity, repeatability, intermediate precision and limits of detection and quantitation; selected validation parameters were also determined for LMWH.
28068519	6	18	theme	quantitation	937:948	arg1	precision					899:907	intermediate precision	886:907	intermediate precision	886:907	A standard validation study on heparin then examined linearity, repeatability, intermediate precision and limits of detection and quantitation; selected validation parameters were also determined for LMWH.
28068519	6	18	theme	quantitation	937:948	arg1	linearity					860:868	linearity	860:868	linearity	860:868	A standard validation study on heparin then examined linearity, repeatability, intermediate precision and limits of detection and quantitation; selected validation parameters were also determined for LMWH.
28068519	6	19	theme	validation	818:827	arg1	study					829:833	A standard validation study	807:833	A standard validation study on heparin	807:844	A standard validation study on heparin then examined linearity, repeatability, intermediate precision and limits of detection and quantitation; selected validation parameters were also determined for LMWH.
28068519	6	20	theme	intermediate	886:897	arg1	precision					899:907	intermediate precision	886:907	intermediate precision	886:907	A standard validation study on heparin then examined linearity, repeatability, intermediate precision and limits of detection and quantitation; selected validation parameters were also determined for LMWH.
28068519	2	21	theme	control	408:414	arg1	applications					416:427	pharmaceutical quality control applications	385:427	pharmaceutical quality control applications	385:427	The focus of the current study was the validation of this procedure to make the 2D-NMR method suitable for pharmaceutical quality control applications.
28068519	6	22	theme	standard	809:816	arg1	study					829:833	A standard validation study	807:833	A standard validation study on heparin	807:844	A standard validation study on heparin then examined linearity, repeatability, intermediate precision and limits of detection and quantitation; selected validation parameters were also determined for LMWH.
28068519	2	23	theme	pharmaceutical	385:398	arg1	applications					416:427	pharmaceutical quality control applications	385:427	pharmaceutical quality control applications	385:427	The focus of the current study was the validation of this procedure to make the 2D-NMR method suitable for pharmaceutical quality control applications.
28068519	2	24	theme	quality	400:406	arg1	applications					416:427	pharmaceutical quality control applications	385:427	pharmaceutical quality control applications	385:427	The focus of the current study was the validation of this procedure to make the 2D-NMR method suitable for pharmaceutical quality control applications.
28068519	4	25	theme	experimental	575:586	arg1	parameters					588:597	Important experimental parameters	565:597	Important experimental parameters	565:597	Important experimental parameters were pulse sequence selection, equilibration interval between pulse trains and temperature.
28068519	5	26	theme	NMR	734:736	arg1	method					738:743	the NMR method	730:743	the NMR method	730:743	These observations were needed so that the NMR method was sufficiently understood to enable continuous improvement.
28068519	1	27	theme	mono-	193:197	arg1	subunits					216:223	the mono- and disaccharide subunits	189:223	the mono- and disaccharide subunits of heparin and low molecular weight heparins (LMWH)	189:275	An NMR HSQC method has recently been proposed for the quantitative determination of the mono- and disaccharide subunits of heparin and low molecular weight heparins (LMWH).
28068519	0	28	theme	quantitative	34:45	arg1	composition					47:57	quantitative composition	34:57	quantitative composition of heparin and low molecular weight heparins	34:102	Qualification of HSQC methods for quantitative composition of heparin and low molecular weight heparins.
28068519	3	29	theme	experimental	486:497	arg1	parameters					499:508	several experimental parameters	478:508	several experimental parameters	478:508	Pre-validation work investigated the effects of several experimental parameters to assess robustness and to optimize critical factors.
28068519	3	30	theme	Pre-validation	430:443	arg1	work					445:448	Pre-validation work	430:448	Pre-validation work	430:448	Pre-validation work investigated the effects of several experimental parameters to assess robustness and to optimize critical factors.
28068519	2	31	theme	suitable	372:379	arg1	method					365:370	the 2D-NMR method	354:370	the 2D-NMR method suitable for pharmaceutical quality control applications	354:427	The focus of the current study was the validation of this procedure to make the 2D-NMR method suitable for pharmaceutical quality control applications.
28068519	0	32	theme	methods	22:28	arg1	Qualification					0:12	Qualification	0:12	Qualification of HSQC methods for quantitative composition of heparin and low molecular weight heparins.	0:103	Qualification of HSQC methods for quantitative composition of heparin and low molecular weight heparins.
28068519	1	33	theme	disaccharide	203:214	arg1	subunits					216:223	the mono- and disaccharide subunits	189:223	the mono- and disaccharide subunits of heparin and low molecular weight heparins (LMWH)	189:275	An NMR HSQC method has recently been proposed for the quantitative determination of the mono- and disaccharide subunits of heparin and low molecular weight heparins (LMWH).
28068519	1	34	theme	NMR	108:110	arg1	method					117:122	An NMR HSQC method	105:122	An NMR HSQC method	105:122	An NMR HSQC method has recently been proposed for the quantitative determination of the mono- and disaccharide subunits of heparin and low molecular weight heparins (LMWH).
28068519	4	35	theme	sequence	610:617	arg1	selection					619:627	pulse sequence selection	604:627	pulse sequence selection	604:627	Important experimental parameters were pulse sequence selection, equilibration interval between pulse trains and temperature.
28068519	4	35	theme	sequence	610:617	arg1	interval					644:651	equilibration interval	630:651	equilibration interval between pulse trains and temperature	630:688	Important experimental parameters were pulse sequence selection, equilibration interval between pulse trains and temperature.
28068519	1	36	theme	HSQC	112:115	arg1	method					117:122	An NMR HSQC method	105:122	An NMR HSQC method	105:122	An NMR HSQC method has recently been proposed for the quantitative determination of the mono- and disaccharide subunits of heparin and low molecular weight heparins (LMWH).
28068519	2	37	theme	study	303:307	arg1	focus					282:286	The focus	278:286	The focus of the current study	278:307	The focus of the current study was the validation of this procedure to make the 2D-NMR method suitable for pharmaceutical quality control applications.
28068519	2	37	theme	study	303:307	arg1	validation					317:326	the validation	313:326	the validation of this procedure to make the 2D-NMR method suitable for pharmaceutical quality control applications	313:427	The focus of the current study was the validation of this procedure to make the 2D-NMR method suitable for pharmaceutical quality control applications.
28068519	1	38	theme	subunits	216:223	arg1	determination					172:184	the quantitative determination	155:184	the quantitative determination of the mono- and disaccharide subunits of heparin and low molecular weight heparins (LMWH)	155:275	An NMR HSQC method has recently been proposed for the quantitative determination of the mono- and disaccharide subunits of heparin and low molecular weight heparins (LMWH).
28068519	2	39	theme	current	295:301	arg1	study					303:307	the current study	291:307	the current study	291:307	The focus of the current study was the validation of this procedure to make the 2D-NMR method suitable for pharmaceutical quality control applications.
28068519	5	40	theme	continuous	783:792	arg1	improvement					794:804	continuous improvement	783:804	continuous improvement	783:804	These observations were needed so that the NMR method was sufficiently understood to enable continuous improvement.
28068519	6	41	from	study	829:833	arg1	heparin					838:844	heparin	838:844	heparin	838:844	A standard validation study on heparin then examined linearity, repeatability, intermediate precision and limits of detection and quantitation; selected validation parameters were also determined for LMWH.
28068519	3	42	dep	investigated	450:461	arg1	optimize					538:545	optimize	538:545	to optimize critical factors	535:562	Pre-validation work investigated the effects of several experimental parameters to assess robustness and to optimize critical factors.
28068519	3	42	dep	investigated	450:461	arg1	assess					513:518	assess	513:518	to assess robustness	510:529	Pre-validation work investigated the effects of several experimental parameters to assess robustness and to optimize critical factors.
28068519	4	43	theme	pulse	661:665	arg1	trains					667:672	pulse trains	661:672	pulse trains	661:672	Important experimental parameters were pulse sequence selection, equilibration interval between pulse trains and temperature.
28068519	2	44	theme	procedure	336:344	arg1	focus					282:286	The focus	278:286	The focus of the current study	278:307	The focus of the current study was the validation of this procedure to make the 2D-NMR method suitable for pharmaceutical quality control applications.
28068519	2	44	theme	procedure	336:344	arg1	validation					317:326	the validation	313:326	the validation of this procedure to make the 2D-NMR method suitable for pharmaceutical quality control applications	313:427	The focus of the current study was the validation of this procedure to make the 2D-NMR method suitable for pharmaceutical quality control applications.
28068519	1	45	theme	heparin	228:234	arg1	subunits					216:223	the mono- and disaccharide subunits	189:223	the mono- and disaccharide subunits of heparin and low molecular weight heparins (LMWH)	189:275	An NMR HSQC method has recently been proposed for the quantitative determination of the mono- and disaccharide subunits of heparin and low molecular weight heparins (LMWH).
28068519	0	46	theme	heparin	62:68	arg1	composition					47:57	quantitative composition	34:57	quantitative composition of heparin and low molecular weight heparins	34:102	Qualification of HSQC methods for quantitative composition of heparin and low molecular weight heparins.
28068519	0	47	theme	HSQC	17:20	arg1	methods					22:28	HSQC methods	17:28	HSQC methods	17:28	Qualification of HSQC methods for quantitative composition of heparin and low molecular weight heparins.
28068519	4	48	theme	equilibration	630:642	arg1	selection					619:627	pulse sequence selection	604:627	pulse sequence selection	604:627	Important experimental parameters were pulse sequence selection, equilibration interval between pulse trains and temperature.
28068519	4	48	theme	equilibration	630:642	arg1	interval					644:651	equilibration interval	630:651	equilibration interval between pulse trains and temperature	630:688	Important experimental parameters were pulse sequence selection, equilibration interval between pulse trains and temperature.
27393889	9	0	theme	process	1309:1315	arg1	intricacies					1279:1289	the intricacies	1275:1289	the intricacies of mineralization process	1275:1315	The applicability of power law model indicates the intricacies of mineralization process.
27393889	10	1	theme	CO2·H2O	1370:1376	arg1	NO3					1379:1381	CO2·H2O, NO3(-)	1370:1384	NO3	1379:1381	During A+P process, OTC and AMP were mineralized to CO2·H2O, NO3(-) and SO4(2-) ions.
27393889	1	2	theme	heterojunctioned	176:191	arg1	BO/BOC					206:211	BO/BOC	206:211	BO/BOC	206:211	In present study, heterojunctioned Bi2O3/BiOCl (BO/BOC) was synthesized via in situ chemical reduction and oxidation of BiOCl nanoplates.
27393889	1	2	theme	heterojunctioned	176:191	arg1	Bi2O3/BiOCl					193:203	heterojunctioned Bi2O3/BiOCl	176:203	heterojunctioned Bi2O3/BiOCl (BO/BOC)	176:212	In present study, heterojunctioned Bi2O3/BiOCl (BO/BOC) was synthesized via in situ chemical reduction and oxidation of BiOCl nanoplates.
27393889	6	3	theme	OTC	1000:1002	arg1	photodegradation					972:987	photodegradation	972:987	photodegradation of AMP and OTC	972:1002	The adsorption process had significant effect on photodegradation of AMP and OTC.
27393889	10	4	theme	SO4	1390:1392	arg1	ions					1398:1401	CO2·H2O, NO3(-) and SO4(2-) ions	1370:1401	CO2·H2O, NO3(-) and SO4(2-) ions	1370:1401	During A+P process, OTC and AMP were mineralized to CO2·H2O, NO3(-) and SO4(2-) ions.
27393889	5	5	theme	solar	910:914	arg1	light					916:920	solar light	910:920	solar light	910:920	The photocatalytic activity of BO/BOC/GSC and BO/BOC/CT was performed for mineralization of ampicillin (AMP) and oxytetracycline (OTC) antibiotics under solar light.
27393889	1	6	theme	BiOCl	278:282	arg1	nanoplates					284:293	BiOCl nanoplates	278:293	BiOCl nanoplates	278:293	In present study, heterojunctioned Bi2O3/BiOCl (BO/BOC) was synthesized via in situ chemical reduction and oxidation of BiOCl nanoplates.
27393889	0	7	theme	sand	129:132	arg1	composite					134:142	graphene sand composite	120:142	graphene sand composite	120:142	Adsorptional photocatalytic mineralization of oxytetracycline and ampicillin antibiotics using Bi2O3/BiOCl supported on graphene sand composite and chitosan.
27393889	5	8	theme	antibiotics	892:902	arg1	mineralization					831:844	mineralization	831:844	mineralization of ampicillin (AMP) and oxytetracycline (OTC) antibiotics	831:902	The photocatalytic activity of BO/BOC/GSC and BO/BOC/CT was performed for mineralization of ampicillin (AMP) and oxytetracycline (OTC) antibiotics under solar light.
27393889	4	9	dep	BET	699:701	arg1	RAMAN					692:696	XRD, EDX, RAMAN, BET	682:701	RAMAN	692:696	The morphology and compositional characteristics of BO/BOC/GSC and BO/BOC/CT were investigated by FESEM, TEM, HRTEM, FTIR, XRD, EDX, RAMAN, BET and UV-visible diffuse reflectance spectral analysis.
27393889	4	9	dep	BET	699:701	arg1	EDX					687:689	XRD, EDX, RAMAN, BET	682:701	EDX	687:689	The morphology and compositional characteristics of BO/BOC/GSC and BO/BOC/CT were investigated by FESEM, TEM, HRTEM, FTIR, XRD, EDX, RAMAN, BET and UV-visible diffuse reflectance spectral analysis.
27393889	1	10	theme	nanoplates	284:293	arg1	oxidation					265:273	oxidation	265:273	oxidation of BiOCl nanoplates	265:293	In present study, heterojunctioned Bi2O3/BiOCl (BO/BOC) was synthesized via in situ chemical reduction and oxidation of BiOCl nanoplates.
27393889	1	10	theme	nanoplates	284:293	arg1	reduction					251:259	in situ chemical reduction	234:259	in situ chemical reduction	234:259	In present study, heterojunctioned Bi2O3/BiOCl (BO/BOC) was synthesized via in situ chemical reduction and oxidation of BiOCl nanoplates.
27393889	0	11	theme	graphene	120:127	arg1	composite					134:142	graphene sand composite	120:142	graphene sand composite	120:142	Adsorptional photocatalytic mineralization of oxytetracycline and ampicillin antibiotics using Bi2O3/BiOCl supported on graphene sand composite and chitosan.
27393889	2	12	from	oxidation	358:366	arg1	solution					376:383	H2O2 solution	371:383	H2O2 solution	371:383	BiOCl was reduced to metallic Bi in KHB4 solution followed by oxidation in H2O2 solution to produce BO/BOC.
27393889	2	13	theme	H2O2	371:374	arg1	solution					376:383	H2O2 solution	371:383	H2O2 solution	371:383	BiOCl was reduced to metallic Bi in KHB4 solution followed by oxidation in H2O2 solution to produce BO/BOC.
27393889	10	14	theme	A+P	1325:1327	arg1	process					1329:1335	A+P process	1325:1335	A+P process	1325:1335	During A+P process, OTC and AMP were mineralized to CO2·H2O, NO3(-) and SO4(2-) ions.
27393889	3	15	theme	impregnation	489:500	arg1	method					502:507	wet impregnation method	485:507	wet impregnation method	485:507	The BO/BOC was supported over graphene sand composite and also on chitosan using wet impregnation method to report BO/BOC/GSC and BO/BOC/CT nanocomposite.
27393889	8	16	theme	degradation	1182:1192	arg1	rate					1194:1197	higher degradation rate	1175:1197	higher degradation rate of investigated antibiotics	1175:1225	Simultaneous adsorption and degradation process (A+P) resulted in higher degradation rate of investigated antibiotics.
27393889	4	17	theme	spectral	738:745	arg1	analysis					747:754	UV-visible diffuse reflectance spectral analysis	707:754	UV-visible diffuse reflectance spectral analysis	707:754	The morphology and compositional characteristics of BO/BOC/GSC and BO/BOC/CT were investigated by FESEM, TEM, HRTEM, FTIR, XRD, EDX, RAMAN, BET and UV-visible diffuse reflectance spectral analysis.
27393889	4	18	theme	BO/BOC/CT	626:634	arg1	morphology					563:572	morphology	563:572	morphology	563:572	The morphology and compositional characteristics of BO/BOC/GSC and BO/BOC/CT were investigated by FESEM, TEM, HRTEM, FTIR, XRD, EDX, RAMAN, BET and UV-visible diffuse reflectance spectral analysis.
27393889	4	18	theme	BO/BOC/CT	626:634	arg1	characteristics					592:606	compositional characteristics	578:606	compositional characteristics	578:606	The morphology and compositional characteristics of BO/BOC/GSC and BO/BOC/CT were investigated by FESEM, TEM, HRTEM, FTIR, XRD, EDX, RAMAN, BET and UV-visible diffuse reflectance spectral analysis.
27393889	5	19	theme	oxytetracycline	870:884	arg1	antibiotics					892:902	ampicillin (AMP) and oxytetracycline (OTC) antibiotics	849:902	ampicillin (AMP) and oxytetracycline (OTC) antibiotics	849:902	The photocatalytic activity of BO/BOC/GSC and BO/BOC/CT was performed for mineralization of ampicillin (AMP) and oxytetracycline (OTC) antibiotics under solar light.
27393889	0	20	theme	photocatalytic	13:26	arg1	mineralization					28:41	Adsorptional photocatalytic mineralization	0:41	Adsorptional photocatalytic mineralization of oxytetracycline and ampicillin antibiotics using Bi2O3/BiOCl	0:105	Adsorptional photocatalytic mineralization of oxytetracycline and ampicillin antibiotics using Bi2O3/BiOCl supported on graphene sand composite and chitosan.
27393889	7	21	theme	second	1086:1091	arg1	kinetics					1099:1106	pseudo second order kinetics	1079:1106	pseudo second order kinetics	1079:1106	The adsorption of both OTC and AMP onto BO/BOC/GSC and BO/BOC/CT followed pseudo second order kinetics.
27393889	6	22	theme	AMP	992:994	arg1	photodegradation					972:987	photodegradation	972:987	photodegradation of AMP and OTC	972:1002	The adsorption process had significant effect on photodegradation of AMP and OTC.
27393889	2	23	theme	KHB4	332:335	arg1	solution					337:344	KHB4 solution	332:344	KHB4 solution followed by oxidation in H2O2 solution to produce BO/BOC	332:401	BiOCl was reduced to metallic Bi in KHB4 solution followed by oxidation in H2O2 solution to produce BO/BOC.
27393889	8	24	theme	Simultaneous	1109:1120	arg1	adsorption					1122:1131	Simultaneous adsorption	1109:1131	Simultaneous adsorption	1109:1131	Simultaneous adsorption and degradation process (A+P) resulted in higher degradation rate of investigated antibiotics.
27393889	3	25	theme	graphene	434:441	arg1	composite					448:456	graphene sand composite	434:456	graphene sand composite	434:456	The BO/BOC was supported over graphene sand composite and also on chitosan using wet impregnation method to report BO/BOC/GSC and BO/BOC/CT nanocomposite.
27393889	0	26	theme	Adsorptional	0:11	arg1	mineralization					28:41	Adsorptional photocatalytic mineralization	0:41	Adsorptional photocatalytic mineralization of oxytetracycline and ampicillin antibiotics using Bi2O3/BiOCl	0:105	Adsorptional photocatalytic mineralization of oxytetracycline and ampicillin antibiotics using Bi2O3/BiOCl supported on graphene sand composite and chitosan.
27393889	4	27	theme	compositional	578:590	arg1	characteristics					592:606	compositional characteristics	578:606	compositional characteristics	578:606	The morphology and compositional characteristics of BO/BOC/GSC and BO/BOC/CT were investigated by FESEM, TEM, HRTEM, FTIR, XRD, EDX, RAMAN, BET and UV-visible diffuse reflectance spectral analysis.
27393889	4	28	theme	UV-visible	707:716	arg1	analysis					747:754	UV-visible diffuse reflectance spectral analysis	707:754	UV-visible diffuse reflectance spectral analysis	707:754	The morphology and compositional characteristics of BO/BOC/GSC and BO/BOC/CT were investigated by FESEM, TEM, HRTEM, FTIR, XRD, EDX, RAMAN, BET and UV-visible diffuse reflectance spectral analysis.
27393889	4	29	theme	reflectance	726:736	arg1	analysis					747:754	UV-visible diffuse reflectance spectral analysis	707:754	UV-visible diffuse reflectance spectral analysis	707:754	The morphology and compositional characteristics of BO/BOC/GSC and BO/BOC/CT were investigated by FESEM, TEM, HRTEM, FTIR, XRD, EDX, RAMAN, BET and UV-visible diffuse reflectance spectral analysis.
27393889	11	30	theme	significant	1444:1454	arg1	efficiency					1464:1473	significant recycle efficiency	1444:1473	significant recycle efficiency	1444:1473	Both BO/BOC/GSC and BO/BOC/CT exhibited significant recycle efficiency.
27393889	3	31	theme	sand	443:446	arg1	composite					448:456	graphene sand composite	434:456	graphene sand composite	434:456	The BO/BOC was supported over graphene sand composite and also on chitosan using wet impregnation method to report BO/BOC/GSC and BO/BOC/CT nanocomposite.
27393889	8	32	theme	antibiotics	1215:1225	arg1	rate					1194:1197	higher degradation rate	1175:1197	higher degradation rate of investigated antibiotics	1175:1225	Simultaneous adsorption and degradation process (A+P) resulted in higher degradation rate of investigated antibiotics.
27393889	11	33	theme	recycle	1456:1462	arg1	efficiency					1464:1473	significant recycle efficiency	1444:1473	significant recycle efficiency	1444:1473	Both BO/BOC/GSC and BO/BOC/CT exhibited significant recycle efficiency.
27393889	5	34	theme	ampicillin	849:858	arg1	antibiotics					892:902	ampicillin (AMP) and oxytetracycline (OTC) antibiotics	849:902	ampicillin (AMP) and oxytetracycline (OTC) antibiotics	849:902	The photocatalytic activity of BO/BOC/GSC and BO/BOC/CT was performed for mineralization of ampicillin (AMP) and oxytetracycline (OTC) antibiotics under solar light.
27393889	8	35	theme	investigated	1202:1213	arg1	antibiotics					1215:1225	investigated antibiotics	1202:1225	investigated antibiotics	1202:1225	Simultaneous adsorption and degradation process (A+P) resulted in higher degradation rate of investigated antibiotics.
27393889	7	36	theme	pseudo	1079:1084	arg1	kinetics					1099:1106	pseudo second order kinetics	1079:1106	pseudo second order kinetics	1079:1106	The adsorption of both OTC and AMP onto BO/BOC/GSC and BO/BOC/CT followed pseudo second order kinetics.
27393889	9	37	theme	power	1249:1253	arg1	model					1259:1263	power law model	1249:1263	power law model	1249:1263	The applicability of power law model indicates the intricacies of mineralization process.
27393889	2	38	theme	metallic	317:324	arg1	Bi					326:327	metallic Bi	317:327	metallic Bi	317:327	BiOCl was reduced to metallic Bi in KHB4 solution followed by oxidation in H2O2 solution to produce BO/BOC.
27393889	7	39	theme	order	1093:1097	arg1	kinetics					1099:1106	pseudo second order kinetics	1079:1106	pseudo second order kinetics	1079:1106	The adsorption of both OTC and AMP onto BO/BOC/GSC and BO/BOC/CT followed pseudo second order kinetics.
27393889	5	40	theme	BO/BOC/GSC	788:797	arg1	activity					776:783	The photocatalytic activity	757:783	The photocatalytic activity of BO/BOC/GSC and BO/BOC/CT	757:811	The photocatalytic activity of BO/BOC/GSC and BO/BOC/CT was performed for mineralization of ampicillin (AMP) and oxytetracycline (OTC) antibiotics under solar light.
27393889	9	41	theme	law	1255:1257	arg1	model					1259:1263	power law model	1249:1263	power law model	1249:1263	The applicability of power law model indicates the intricacies of mineralization process.
27393889	1	42	theme	in	234:235	arg1	reduction					251:259	in situ chemical reduction	234:259	in situ chemical reduction	234:259	In present study, heterojunctioned Bi2O3/BiOCl (BO/BOC) was synthesized via in situ chemical reduction and oxidation of BiOCl nanoplates.
27393889	0	43	theme	oxytetracycline	46:60	arg1	antibiotics					77:87	oxytetracycline and ampicillin antibiotics	46:87	oxytetracycline and ampicillin antibiotics using Bi2O3/BiOCl	46:105	Adsorptional photocatalytic mineralization of oxytetracycline and ampicillin antibiotics using Bi2O3/BiOCl supported on graphene sand composite and chitosan.
27393889	4	44	theme	BO/BOC/GSC	611:620	arg1	morphology					563:572	morphology	563:572	morphology	563:572	The morphology and compositional characteristics of BO/BOC/GSC and BO/BOC/CT were investigated by FESEM, TEM, HRTEM, FTIR, XRD, EDX, RAMAN, BET and UV-visible diffuse reflectance spectral analysis.
27393889	4	44	theme	BO/BOC/GSC	611:620	arg1	characteristics					592:606	compositional characteristics	578:606	compositional characteristics	578:606	The morphology and compositional characteristics of BO/BOC/GSC and BO/BOC/CT were investigated by FESEM, TEM, HRTEM, FTIR, XRD, EDX, RAMAN, BET and UV-visible diffuse reflectance spectral analysis.
27393889	9	45	theme	model	1259:1263	arg1	applicability					1232:1244	The applicability	1228:1244	The applicability of power law model	1228:1263	The applicability of power law model indicates the intricacies of mineralization process.
27393889	8	46	theme	higher	1175:1180	arg1	rate					1194:1197	higher degradation rate	1175:1197	higher degradation rate of investigated antibiotics	1175:1225	Simultaneous adsorption and degradation process (A+P) resulted in higher degradation rate of investigated antibiotics.
27393889	0	47	theme	antibiotics	77:87	arg1	mineralization					28:41	Adsorptional photocatalytic mineralization	0:41	Adsorptional photocatalytic mineralization of oxytetracycline and ampicillin antibiotics using Bi2O3/BiOCl	0:105	Adsorptional photocatalytic mineralization of oxytetracycline and ampicillin antibiotics using Bi2O3/BiOCl supported on graphene sand composite and chitosan.
27393889	6	48	theme	significant	950:960	arg1	effect					962:967	significant effect	950:967	significant effect	950:967	The adsorption process had significant effect on photodegradation of AMP and OTC.
27393889	5	49	theme	photocatalytic	761:774	arg1	activity					776:783	The photocatalytic activity	757:783	The photocatalytic activity of BO/BOC/GSC and BO/BOC/CT	757:811	The photocatalytic activity of BO/BOC/GSC and BO/BOC/CT was performed for mineralization of ampicillin (AMP) and oxytetracycline (OTC) antibiotics under solar light.
27393889	7	50	theme	OTC	1028:1030	arg1	adsorption					1009:1018	The adsorption	1005:1018	The adsorption of both OTC and AMP onto BO/BOC/GSC and BO/BOC/CT	1005:1068	The adsorption of both OTC and AMP onto BO/BOC/GSC and BO/BOC/CT followed pseudo second order kinetics.
27393889	0	51	theme	ampicillin	66:75	arg1	antibiotics					77:87	oxytetracycline and ampicillin antibiotics	46:87	oxytetracycline and ampicillin antibiotics using Bi2O3/BiOCl	46:105	Adsorptional photocatalytic mineralization of oxytetracycline and ampicillin antibiotics using Bi2O3/BiOCl supported on graphene sand composite and chitosan.
27393889	6	52	theme	adsorption	927:936	arg1	process					938:944	The adsorption process	923:944	The adsorption process	923:944	The adsorption process had significant effect on photodegradation of AMP and OTC.
27393889	8	53	theme	degradation	1137:1147	arg1	A+P					1158:1160	A+P	1158:1160	A+P	1158:1160	Simultaneous adsorption and degradation process (A+P) resulted in higher degradation rate of investigated antibiotics.
27393889	8	53	theme	degradation	1137:1147	arg1	process					1149:1155	degradation process	1137:1155	degradation process (A+P)	1137:1161	Simultaneous adsorption and degradation process (A+P) resulted in higher degradation rate of investigated antibiotics.
27393889	1	54	dep	in	234:235	arg1	situ					237:240	situ	237:240	situ	237:240	In present study, heterojunctioned Bi2O3/BiOCl (BO/BOC) was synthesized via in situ chemical reduction and oxidation of BiOCl nanoplates.
27393889	3	55	theme	wet	485:487	arg1	method					502:507	wet impregnation method	485:507	wet impregnation method	485:507	The BO/BOC was supported over graphene sand composite and also on chitosan using wet impregnation method to report BO/BOC/GSC and BO/BOC/CT nanocomposite.
27393889	1	56	theme	chemical	242:249	arg1	reduction					251:259	in situ chemical reduction	234:259	in situ chemical reduction	234:259	In present study, heterojunctioned Bi2O3/BiOCl (BO/BOC) was synthesized via in situ chemical reduction and oxidation of BiOCl nanoplates.
27393889	7	57	theme	AMP	1036:1038	arg1	adsorption					1009:1018	The adsorption	1005:1018	The adsorption of both OTC and AMP onto BO/BOC/GSC and BO/BOC/CT	1005:1068	The adsorption of both OTC and AMP onto BO/BOC/GSC and BO/BOC/CT followed pseudo second order kinetics.
27393889	6	58	contain	had	946:948	arg1	process					938:944	The adsorption process	923:944	The adsorption process	923:944	The adsorption process had significant effect on photodegradation of AMP and OTC.
27393889	6	58	contain	had	946:948	arg2	effect					962:967	significant effect	950:967	significant effect	950:967	The adsorption process had significant effect on photodegradation of AMP and OTC.
27393889	1	59	theme	present	161:167	arg1	study					169:173	present study	161:173	present study	161:173	In present study, heterojunctioned Bi2O3/BiOCl (BO/BOC) was synthesized via in situ chemical reduction and oxidation of BiOCl nanoplates.
27393889	4	60	theme	diffuse	718:724	arg1	analysis					747:754	UV-visible diffuse reflectance spectral analysis	707:754	UV-visible diffuse reflectance spectral analysis	707:754	The morphology and compositional characteristics of BO/BOC/GSC and BO/BOC/CT were investigated by FESEM, TEM, HRTEM, FTIR, XRD, EDX, RAMAN, BET and UV-visible diffuse reflectance spectral analysis.
27393889	9	61	theme	mineralization	1294:1307	arg1	process					1309:1315	mineralization process	1294:1315	mineralization process	1294:1315	The applicability of power law model indicates the intricacies of mineralization process.
27393889	10	62	theme	NO3	1379:1381	arg1	ions					1398:1401	CO2·H2O, NO3(-) and SO4(2-) ions	1370:1401	CO2·H2O, NO3(-) and SO4(2-) ions	1370:1401	During A+P process, OTC and AMP were mineralized to CO2·H2O, NO3(-) and SO4(2-) ions.
27393889	5	63	theme	BO/BOC/CT	803:811	arg1	activity					776:783	The photocatalytic activity	757:783	The photocatalytic activity of BO/BOC/GSC and BO/BOC/CT	757:811	The photocatalytic activity of BO/BOC/GSC and BO/BOC/CT was performed for mineralization of ampicillin (AMP) and oxytetracycline (OTC) antibiotics under solar light.
27393889	4	64	dep	morphology	563:572	arg1	The					559:561	The	559:561	The	559:561	The morphology and compositional characteristics of BO/BOC/GSC and BO/BOC/CT were investigated by FESEM, TEM, HRTEM, FTIR, XRD, EDX, RAMAN, BET and UV-visible diffuse reflectance spectral analysis.
27393889	3	65	theme	BO/BOC/CT	534:542	arg1	nanocomposite					544:556	BO/BOC/CT nanocomposite	534:556	BO/BOC/CT nanocomposite	534:556	The BO/BOC was supported over graphene sand composite and also on chitosan using wet impregnation method to report BO/BOC/GSC and BO/BOC/CT nanocomposite.
28522398	0	0	theme	low	77:79	arg1	substitution					81:92	low substitution	77:92	low substitution under microwave assistance	77:119	Study on the synthesis and physicochemical properties of starch acetate with low substitution under microwave assistance.
28522398	7	1	theme	microwave	1362:1370	arg1	technology					1372:1381	microwave technology	1362:1381	microwave technology that can destroy the extent of the crystal and amorphous regions	1362:1446	Crystal structure was observed by X-ray diffraction, and the ACS can determine the level of microwave technology that can destroy the extent of the crystal and amorphous regions.
28522398	4	2	theme	chemical	669:676	arg1	better					883:888	better	883:888	better	883:888	These optimal conditions predicted by RSM were confirmed that the degree of substitution (DS) of acetate starch is 0.0691mg/g and the physical and chemical properties of natural corn starch (NCS) and corn starch acetate (ACS) were further studied.The transparency, water separation, water absorption, expansion force, and solubility of ACS low substitution are better than NCS, while the NCS's hydrolysis percentage is higher than ACS, which indicate that the modified corn starch has better performance than native corn starch.
28522398	4	2	theme	chemical	669:676	arg1	properties					678:687	the physical and chemical properties	652:687	the physical and chemical properties of natural corn starch (NCS) and corn starch acetate (ACS)	652:746	These optimal conditions predicted by RSM were confirmed that the degree of substitution (DS) of acetate starch is 0.0691mg/g and the physical and chemical properties of natural corn starch (NCS) and corn starch acetate (ACS) were further studied.The transparency, water separation, water absorption, expansion force, and solubility of ACS low substitution are better than NCS, while the NCS's hydrolysis percentage is higher than ACS, which indicate that the modified corn starch has better performance than native corn starch.
28522398	2	3	theme	reaction	373:380	arg1	conditions					382:391	the reaction conditions	369:391	the reaction conditions	369:391	A three-level-three-factorial Central Composite Design using Response Surface Methodology (RSM) was employed to optimize the reaction conditions.
28522398	7	4	theme	amorphous	1430:1438	arg1	regions					1440:1446	the crystal and amorphous regions	1414:1446	the crystal and amorphous regions	1414:1446	Crystal structure was observed by X-ray diffraction, and the ACS can determine the level of microwave technology that can destroy the extent of the crystal and amorphous regions.
28522398	7	5	theme	X-ray	1304:1308	arg1	diffraction					1310:1320	X-ray diffraction	1304:1320	X-ray diffraction	1304:1320	Crystal structure was observed by X-ray diffraction, and the ACS can determine the level of microwave technology that can destroy the extent of the crystal and amorphous regions.
28522398	1	6	theme	physicochemical	151:165	arg1	properties					167:176	physicochemical properties	151:176	physicochemical properties	151:176	In this study, synthesis and physicochemical properties of starch acetate with low substitution under microwave were studied.
28522398	4	7	theme	modified	982:989	arg1	starch					996:1001	the modified corn starch	978:1001	the modified corn starch	978:1001	These optimal conditions predicted by RSM were confirmed that the degree of substitution (DS) of acetate starch is 0.0691mg/g and the physical and chemical properties of natural corn starch (NCS) and corn starch acetate (ACS) were further studied.The transparency, water separation, water absorption, expansion force, and solubility of ACS low substitution are better than NCS, while the NCS's hydrolysis percentage is higher than ACS, which indicate that the modified corn starch has better performance than native corn starch.
28522398	5	8	theme	scanning	1117:1124	arg1	SEM					1147:1149	SEM	1147:1149	SEM	1147:1149	The surface morphology of the corn starch acetate was examined by scanning electron microscope (SEM), which showed that it had a smooth surface and a spherical and polygonal shape.
28522398	5	8	theme	scanning	1117:1124	arg1	microscope					1135:1144	scanning electron microscope	1117:1144	scanning electron microscope (SEM)	1117:1150	The surface morphology of the corn starch acetate was examined by scanning electron microscope (SEM), which showed that it had a smooth surface and a spherical and polygonal shape.
28522398	5	9	theme	surface	1055:1061	arg1	morphology					1063:1072	The surface morphology	1051:1072	The surface morphology of the corn starch acetate	1051:1099	The surface morphology of the corn starch acetate was examined by scanning electron microscope (SEM), which showed that it had a smooth surface and a spherical and polygonal shape.
28522398	8	10	theme	acetylation	1550:1560	arg1	bonding					1562:1568	acetylation bonding	1550:1568	acetylation bonding successfully	1550:1581	Fourier transform infrared (FTIR) spectroscopy shows that around 1750cm-1 carbonyl signal determines acetylation bonding successfully.
28522398	2	11	theme	Surface	318:324	arg1	RSM					339:341	RSM	339:341	RSM	339:341	A three-level-three-factorial Central Composite Design using Response Surface Methodology (RSM) was employed to optimize the reaction conditions.
28522398	2	11	theme	Surface	318:324	arg1	Methodology					326:336	Response Surface Methodology	309:336	Response Surface Methodology (RSM)	309:342	A three-level-three-factorial Central Composite Design using Response Surface Methodology (RSM) was employed to optimize the reaction conditions.
28522398	5	12	theme	electron	1126:1133	arg1	SEM					1147:1149	SEM	1147:1149	SEM	1147:1149	The surface morphology of the corn starch acetate was examined by scanning electron microscope (SEM), which showed that it had a smooth surface and a spherical and polygonal shape.
28522398	5	12	theme	electron	1126:1133	arg1	microscope					1135:1144	scanning electron microscope	1117:1144	scanning electron microscope (SEM)	1117:1150	The surface morphology of the corn starch acetate was examined by scanning electron microscope (SEM), which showed that it had a smooth surface and a spherical and polygonal shape.
28522398	4	13	theme	expansion	823:831	arg1	force					833:837	expansion force	823:837	expansion force	823:837	These optimal conditions predicted by RSM were confirmed that the degree of substitution (DS) of acetate starch is 0.0691mg/g and the physical and chemical properties of natural corn starch (NCS) and corn starch acetate (ACS) were further studied.The transparency, water separation, water absorption, expansion force, and solubility of ACS low substitution are better than NCS, while the NCS's hydrolysis percentage is higher than ACS, which indicate that the modified corn starch has better performance than native corn starch.
28522398	2	14	theme	Response	309:316	arg1	RSM					339:341	RSM	339:341	RSM	339:341	A three-level-three-factorial Central Composite Design using Response Surface Methodology (RSM) was employed to optimize the reaction conditions.
28522398	2	14	theme	Response	309:316	arg1	Methodology					326:336	Response Surface Methodology	309:336	Response Surface Methodology (RSM)	309:342	A three-level-three-factorial Central Composite Design using Response Surface Methodology (RSM) was employed to optimize the reaction conditions.
28522398	5	15	theme	polygonal	1215:1223	arg1	shape					1225:1229	a spherical and polygonal shape	1199:1229	shape	1225:1229	The surface morphology of the corn starch acetate was examined by scanning electron microscope (SEM), which showed that it had a smooth surface and a spherical and polygonal shape.
28522398	8	16	theme	around	1507:1512	arg1	signal					1532:1537	around 1750cm-1 carbonyl signal	1507:1537	around 1750cm-1 carbonyl signal	1507:1537	Fourier transform infrared (FTIR) spectroscopy shows that around 1750cm-1 carbonyl signal determines acetylation bonding successfully.
28522398	4	17	contain	has	1003:1005	arg1	starch					996:1001	the modified corn starch	978:1001	the modified corn starch	978:1001	These optimal conditions predicted by RSM were confirmed that the degree of substitution (DS) of acetate starch is 0.0691mg/g and the physical and chemical properties of natural corn starch (NCS) and corn starch acetate (ACS) were further studied.The transparency, water separation, water absorption, expansion force, and solubility of ACS low substitution are better than NCS, while the NCS's hydrolysis percentage is higher than ACS, which indicate that the modified corn starch has better performance than native corn starch.
28522398	4	17	contain	has	1003:1005	arg2	performance					1014:1024	better performance	1007:1024	better performance than native corn starch	1007:1048	These optimal conditions predicted by RSM were confirmed that the degree of substitution (DS) of acetate starch is 0.0691mg/g and the physical and chemical properties of natural corn starch (NCS) and corn starch acetate (ACS) were further studied.The transparency, water separation, water absorption, expansion force, and solubility of ACS low substitution are better than NCS, while the NCS's hydrolysis percentage is higher than ACS, which indicate that the modified corn starch has better performance than native corn starch.
28522398	1	18	theme	low	201:203	arg1	substitution					205:216	low substitution	201:216	low substitution	201:216	In this study, synthesis and physicochemical properties of starch acetate with low substitution under microwave were studied.
28522398	3	19	theme	100W	516:519	arg1	power					507:511	microwave power	497:511	microwave power of 100W	497:519	The optimal parameters are as follows: amount of acetic anhydride of 12%, radiation time of 11min, and microwave power of 100W.
28522398	3	19	theme	100W	516:519	arg1	anhydride					450:458	acetic anhydride	443:458	acetic anhydride of 12%	443:465	The optimal parameters are as follows: amount of acetic anhydride of 12%, radiation time of 11min, and microwave power of 100W.
28522398	3	19	theme	100W	516:519	arg1	time					478:481	radiation time	468:481	radiation time of 11min	468:490	The optimal parameters are as follows: amount of acetic anhydride of 12%, radiation time of 11min, and microwave power of 100W.
28522398	3	19	theme	100W	516:519	arg1	11min					486:490	11min	486:490	11min	486:490	The optimal parameters are as follows: amount of acetic anhydride of 12%, radiation time of 11min, and microwave power of 100W.
28522398	3	19	theme	100W	516:519	arg1	100W					516:519	100W	516:519	100W	516:519	The optimal parameters are as follows: amount of acetic anhydride of 12%, radiation time of 11min, and microwave power of 100W.
28522398	3	19	theme	100W	516:519	arg1	amount					433:438	amount	433:438	amount of acetic anhydride of 12%	433:465	The optimal parameters are as follows: amount of acetic anhydride of 12%, radiation time of 11min, and microwave power of 100W.
28522398	5	20	theme	smooth	1180:1185	arg1	surface					1187:1193	a smooth surface	1178:1193	a smooth surface	1178:1193	The surface morphology of the corn starch acetate was examined by scanning electron microscope (SEM), which showed that it had a smooth surface and a spherical and polygonal shape.
28522398	3	21	theme	anhydride	450:458	arg1	power					507:511	microwave power	497:511	microwave power of 100W	497:519	The optimal parameters are as follows: amount of acetic anhydride of 12%, radiation time of 11min, and microwave power of 100W.
28522398	3	21	theme	anhydride	450:458	arg1	anhydride					450:458	acetic anhydride	443:458	acetic anhydride of 12%	443:465	The optimal parameters are as follows: amount of acetic anhydride of 12%, radiation time of 11min, and microwave power of 100W.
28522398	3	21	theme	anhydride	450:458	arg1	time					478:481	radiation time	468:481	radiation time of 11min	468:490	The optimal parameters are as follows: amount of acetic anhydride of 12%, radiation time of 11min, and microwave power of 100W.
28522398	3	21	theme	anhydride	450:458	arg1	11min					486:490	11min	486:490	11min	486:490	The optimal parameters are as follows: amount of acetic anhydride of 12%, radiation time of 11min, and microwave power of 100W.
28522398	3	21	theme	anhydride	450:458	arg1	100W					516:519	100W	516:519	100W	516:519	The optimal parameters are as follows: amount of acetic anhydride of 12%, radiation time of 11min, and microwave power of 100W.
28522398	3	21	theme	anhydride	450:458	arg1	amount					433:438	amount	433:438	amount of acetic anhydride of 12%	433:465	The optimal parameters are as follows: amount of acetic anhydride of 12%, radiation time of 11min, and microwave power of 100W.
28522398	0	22	theme	microwave	100:108	arg1	assistance					110:119	microwave assistance	100:119	microwave assistance	100:119	Study on the synthesis and physicochemical properties of starch acetate with low substitution under microwave assistance.
28522398	3	23	dep	follows	424:430	arg1	power					507:511	microwave power	497:511	microwave power of 100W	497:519	The optimal parameters are as follows: amount of acetic anhydride of 12%, radiation time of 11min, and microwave power of 100W.
28522398	3	23	dep	follows	424:430	arg1	anhydride					450:458	acetic anhydride	443:458	acetic anhydride of 12%	443:465	The optimal parameters are as follows: amount of acetic anhydride of 12%, radiation time of 11min, and microwave power of 100W.
28522398	3	23	dep	follows	424:430	arg1	time					478:481	radiation time	468:481	radiation time of 11min	468:490	The optimal parameters are as follows: amount of acetic anhydride of 12%, radiation time of 11min, and microwave power of 100W.
28522398	3	23	dep	follows	424:430	arg1	11min					486:490	11min	486:490	11min	486:490	The optimal parameters are as follows: amount of acetic anhydride of 12%, radiation time of 11min, and microwave power of 100W.
28522398	3	23	dep	follows	424:430	arg1	100W					516:519	100W	516:519	100W	516:519	The optimal parameters are as follows: amount of acetic anhydride of 12%, radiation time of 11min, and microwave power of 100W.
28522398	3	23	dep	follows	424:430	arg1	amount					433:438	amount	433:438	amount of acetic anhydride of 12%	433:465	The optimal parameters are as follows: amount of acetic anhydride of 12%, radiation time of 11min, and microwave power of 100W.
28522398	4	24	theme	starch	727:732	arg1	ACS					743:745	ACS	743:745	ACS	743:745	These optimal conditions predicted by RSM were confirmed that the degree of substitution (DS) of acetate starch is 0.0691mg/g and the physical and chemical properties of natural corn starch (NCS) and corn starch acetate (ACS) were further studied.The transparency, water separation, water absorption, expansion force, and solubility of ACS low substitution are better than NCS, while the NCS's hydrolysis percentage is higher than ACS, which indicate that the modified corn starch has better performance than native corn starch.
28522398	4	24	theme	starch	727:732	arg1	acetate					734:740	corn starch acetate	722:740	corn starch acetate (ACS)	722:746	These optimal conditions predicted by RSM were confirmed that the degree of substitution (DS) of acetate starch is 0.0691mg/g and the physical and chemical properties of natural corn starch (NCS) and corn starch acetate (ACS) were further studied.The transparency, water separation, water absorption, expansion force, and solubility of ACS low substitution are better than NCS, while the NCS's hydrolysis percentage is higher than ACS, which indicate that the modified corn starch has better performance than native corn starch.
28522398	4	25	theme	starch	627:632	arg1	DS					612:613	DS	612:613	DS	612:613	These optimal conditions predicted by RSM were confirmed that the degree of substitution (DS) of acetate starch is 0.0691mg/g and the physical and chemical properties of natural corn starch (NCS) and corn starch acetate (ACS) were further studied.The transparency, water separation, water absorption, expansion force, and solubility of ACS low substitution are better than NCS, while the NCS's hydrolysis percentage is higher than ACS, which indicate that the modified corn starch has better performance than native corn starch.
28522398	4	25	theme	starch	627:632	arg1	substitution					598:609	substitution	598:609	substitution (DS) of acetate starch	598:632	These optimal conditions predicted by RSM were confirmed that the degree of substitution (DS) of acetate starch is 0.0691mg/g and the physical and chemical properties of natural corn starch (NCS) and corn starch acetate (ACS) were further studied.The transparency, water separation, water absorption, expansion force, and solubility of ACS low substitution are better than NCS, while the NCS's hydrolysis percentage is higher than ACS, which indicate that the modified corn starch has better performance than native corn starch.
28522398	7	26	theme	crystal	1418:1424	arg1	regions					1440:1446	the crystal and amorphous regions	1414:1446	the crystal and amorphous regions	1414:1446	Crystal structure was observed by X-ray diffraction, and the ACS can determine the level of microwave technology that can destroy the extent of the crystal and amorphous regions.
28522398	4	27	theme	water	805:809	arg1	absorption					811:820	water absorption	805:820	water absorption	805:820	These optimal conditions predicted by RSM were confirmed that the degree of substitution (DS) of acetate starch is 0.0691mg/g and the physical and chemical properties of natural corn starch (NCS) and corn starch acetate (ACS) were further studied.The transparency, water separation, water absorption, expansion force, and solubility of ACS low substitution are better than NCS, while the NCS's hydrolysis percentage is higher than ACS, which indicate that the modified corn starch has better performance than native corn starch.
28522398	1	28	theme	starch	181:186	arg1	acetate					188:194	starch acetate	181:194	starch acetate with low substitution	181:216	In this study, synthesis and physicochemical properties of starch acetate with low substitution under microwave were studied.
28522398	5	29	theme	starch	1086:1091	arg1	acetate					1093:1099	the corn starch acetate	1077:1099	the corn starch acetate	1077:1099	The surface morphology of the corn starch acetate was examined by scanning electron microscope (SEM), which showed that it had a smooth surface and a spherical and polygonal shape.
28522398	4	30	theme	native	1031:1036	arg1	starch					1043:1048	native corn starch	1031:1048	native corn starch	1031:1048	These optimal conditions predicted by RSM were confirmed that the degree of substitution (DS) of acetate starch is 0.0691mg/g and the physical and chemical properties of natural corn starch (NCS) and corn starch acetate (ACS) were further studied.The transparency, water separation, water absorption, expansion force, and solubility of ACS low substitution are better than NCS, while the NCS's hydrolysis percentage is higher than ACS, which indicate that the modified corn starch has better performance than native corn starch.
28522398	4	31	theme	starch	705:710	arg1	better					883:888	better	883:888	better	883:888	These optimal conditions predicted by RSM were confirmed that the degree of substitution (DS) of acetate starch is 0.0691mg/g and the physical and chemical properties of natural corn starch (NCS) and corn starch acetate (ACS) were further studied.The transparency, water separation, water absorption, expansion force, and solubility of ACS low substitution are better than NCS, while the NCS's hydrolysis percentage is higher than ACS, which indicate that the modified corn starch has better performance than native corn starch.
28522398	4	31	theme	starch	705:710	arg1	0.0691mg/g					637:646	0.0691mg/g	637:646	0.0691mg/g	637:646	These optimal conditions predicted by RSM were confirmed that the degree of substitution (DS) of acetate starch is 0.0691mg/g and the physical and chemical properties of natural corn starch (NCS) and corn starch acetate (ACS) were further studied.The transparency, water separation, water absorption, expansion force, and solubility of ACS low substitution are better than NCS, while the NCS's hydrolysis percentage is higher than ACS, which indicate that the modified corn starch has better performance than native corn starch.
28522398	4	31	theme	starch	705:710	arg1	properties					678:687	the physical and chemical properties	652:687	the physical and chemical properties of natural corn starch (NCS) and corn starch acetate (ACS)	652:746	These optimal conditions predicted by RSM were confirmed that the degree of substitution (DS) of acetate starch is 0.0691mg/g and the physical and chemical properties of natural corn starch (NCS) and corn starch acetate (ACS) were further studied.The transparency, water separation, water absorption, expansion force, and solubility of ACS low substitution are better than NCS, while the NCS's hydrolysis percentage is higher than ACS, which indicate that the modified corn starch has better performance than native corn starch.
28522398	4	32	theme	corn	722:725	arg1	ACS					743:745	ACS	743:745	ACS	743:745	These optimal conditions predicted by RSM were confirmed that the degree of substitution (DS) of acetate starch is 0.0691mg/g and the physical and chemical properties of natural corn starch (NCS) and corn starch acetate (ACS) were further studied.The transparency, water separation, water absorption, expansion force, and solubility of ACS low substitution are better than NCS, while the NCS's hydrolysis percentage is higher than ACS, which indicate that the modified corn starch has better performance than native corn starch.
28522398	4	32	theme	corn	722:725	arg1	acetate					734:740	corn starch acetate	722:740	corn starch acetate (ACS)	722:746	These optimal conditions predicted by RSM were confirmed that the degree of substitution (DS) of acetate starch is 0.0691mg/g and the physical and chemical properties of natural corn starch (NCS) and corn starch acetate (ACS) were further studied.The transparency, water separation, water absorption, expansion force, and solubility of ACS low substitution are better than NCS, while the NCS's hydrolysis percentage is higher than ACS, which indicate that the modified corn starch has better performance than native corn starch.
28522398	1	33	theme	acetate	188:194	arg1	synthesis					137:145	synthesis	137:145	synthesis	137:145	In this study, synthesis and physicochemical properties of starch acetate with low substitution under microwave were studied.
28522398	1	33	theme	acetate	188:194	arg1	properties					167:176	physicochemical properties	151:176	physicochemical properties	151:176	In this study, synthesis and physicochemical properties of starch acetate with low substitution under microwave were studied.
28522398	5	34	theme	acetate	1093:1099	arg1	morphology					1063:1072	The surface morphology	1051:1072	The surface morphology of the corn starch acetate	1051:1099	The surface morphology of the corn starch acetate was examined by scanning electron microscope (SEM), which showed that it had a smooth surface and a spherical and polygonal shape.
28522398	4	35	theme	low	862:864	arg1	substitution					866:877	ACS low substitution	858:877	ACS low substitution	858:877	These optimal conditions predicted by RSM were confirmed that the degree of substitution (DS) of acetate starch is 0.0691mg/g and the physical and chemical properties of natural corn starch (NCS) and corn starch acetate (ACS) were further studied.The transparency, water separation, water absorption, expansion force, and solubility of ACS low substitution are better than NCS, while the NCS's hydrolysis percentage is higher than ACS, which indicate that the modified corn starch has better performance than native corn starch.
28522398	5	36	contain	had	1174:1176	arg2	surface					1187:1193	a smooth surface	1178:1193	a smooth surface	1178:1193	The surface morphology of the corn starch acetate was examined by scanning electron microscope (SEM), which showed that it had a smooth surface and a spherical and polygonal shape.
28522398	5	36	contain	had	1174:1176	arg2	shape					1225:1229	a spherical and polygonal shape	1199:1229	shape	1225:1229	The surface morphology of the corn starch acetate was examined by scanning electron microscope (SEM), which showed that it had a smooth surface and a spherical and polygonal shape.
28522398	5	36	contain	had	1174:1176	arg1	it					1171:1172	it	1171:1172	it	1171:1172	The surface morphology of the corn starch acetate was examined by scanning electron microscope (SEM), which showed that it had a smooth surface and a spherical and polygonal shape.
28522398	3	37	theme	optimal	398:404	arg1	parameters					406:415	The optimal parameters	394:415	The optimal parameters	394:415	The optimal parameters are as follows: amount of acetic anhydride of 12%, radiation time of 11min, and microwave power of 100W.
28522398	7	38	theme	regions	1440:1446	arg1	extent					1404:1409	the extent	1400:1409	the extent of the crystal and amorphous regions	1400:1446	Crystal structure was observed by X-ray diffraction, and the ACS can determine the level of microwave technology that can destroy the extent of the crystal and amorphous regions.
28522398	4	39	theme	ACS	858:860	arg1	substitution					866:877	ACS low substitution	858:877	ACS low substitution	858:877	These optimal conditions predicted by RSM were confirmed that the degree of substitution (DS) of acetate starch is 0.0691mg/g and the physical and chemical properties of natural corn starch (NCS) and corn starch acetate (ACS) were further studied.The transparency, water separation, water absorption, expansion force, and solubility of ACS low substitution are better than NCS, while the NCS's hydrolysis percentage is higher than ACS, which indicate that the modified corn starch has better performance than native corn starch.
28522398	8	40	dep	infrared	1467:1474	arg1	FTIR					1477:1480	FTIR	1477:1480	FTIR	1477:1480	Fourier transform infrared (FTIR) spectroscopy shows that around 1750cm-1 carbonyl signal determines acetylation bonding successfully.
28522398	3	41	theme	microwave	497:505	arg1	power					507:511	microwave power	497:511	microwave power of 100W	497:519	The optimal parameters are as follows: amount of acetic anhydride of 12%, radiation time of 11min, and microwave power of 100W.
28522398	4	42	theme	corn	1038:1041	arg1	starch					1043:1048	native corn starch	1031:1048	native corn starch	1031:1048	These optimal conditions predicted by RSM were confirmed that the degree of substitution (DS) of acetate starch is 0.0691mg/g and the physical and chemical properties of natural corn starch (NCS) and corn starch acetate (ACS) were further studied.The transparency, water separation, water absorption, expansion force, and solubility of ACS low substitution are better than NCS, while the NCS's hydrolysis percentage is higher than ACS, which indicate that the modified corn starch has better performance than native corn starch.
28522398	3	43	theme	radiation	468:476	arg1	time					478:481	radiation time	468:481	radiation time of 11min	468:490	The optimal parameters are as follows: amount of acetic anhydride of 12%, radiation time of 11min, and microwave power of 100W.
28522398	0	44	theme	physicochemical	27:41	arg1	properties					43:52	physicochemical properties	27:52	physicochemical properties	27:52	Study on the synthesis and physicochemical properties of starch acetate with low substitution under microwave assistance.
28522398	8	45	theme	infrared	1467:1474	arg1	spectroscopy					1483:1494	infrared (FTIR) spectroscopy	1467:1494	infrared (FTIR) spectroscopy	1467:1494	Fourier transform infrared (FTIR) spectroscopy shows that around 1750cm-1 carbonyl signal determines acetylation bonding successfully.
28522398	4	46	theme	studied.The	761:771	arg1	transparency					773:784	further studied.The transparency	753:784	further studied.The transparency	753:784	These optimal conditions predicted by RSM were confirmed that the degree of substitution (DS) of acetate starch is 0.0691mg/g and the physical and chemical properties of natural corn starch (NCS) and corn starch acetate (ACS) were further studied.The transparency, water separation, water absorption, expansion force, and solubility of ACS low substitution are better than NCS, while the NCS's hydrolysis percentage is higher than ACS, which indicate that the modified corn starch has better performance than native corn starch.
28522398	3	47	theme	acetic	443:448	arg1	anhydride					450:458	acetic anhydride	443:458	acetic anhydride of 12%	443:465	The optimal parameters are as follows: amount of acetic anhydride of 12%, radiation time of 11min, and microwave power of 100W.
28522398	4	48	theme	acetate	619:625	arg1	starch					627:632	acetate starch	619:632	acetate starch	619:632	These optimal conditions predicted by RSM were confirmed that the degree of substitution (DS) of acetate starch is 0.0691mg/g and the physical and chemical properties of natural corn starch (NCS) and corn starch acetate (ACS) were further studied.The transparency, water separation, water absorption, expansion force, and solubility of ACS low substitution are better than NCS, while the NCS's hydrolysis percentage is higher than ACS, which indicate that the modified corn starch has better performance than native corn starch.
28522398	4	49	theme	hydrolysis	916:925	arg1	higher					941:946	higher	941:946	higher	941:946	These optimal conditions predicted by RSM were confirmed that the degree of substitution (DS) of acetate starch is 0.0691mg/g and the physical and chemical properties of natural corn starch (NCS) and corn starch acetate (ACS) were further studied.The transparency, water separation, water absorption, expansion force, and solubility of ACS low substitution are better than NCS, while the NCS's hydrolysis percentage is higher than ACS, which indicate that the modified corn starch has better performance than native corn starch.
28522398	4	49	theme	hydrolysis	916:925	arg1	percentage					927:936	the NCS's hydrolysis percentage	906:936	the NCS's hydrolysis percentage	906:936	These optimal conditions predicted by RSM were confirmed that the degree of substitution (DS) of acetate starch is 0.0691mg/g and the physical and chemical properties of natural corn starch (NCS) and corn starch acetate (ACS) were further studied.The transparency, water separation, water absorption, expansion force, and solubility of ACS low substitution are better than NCS, while the NCS's hydrolysis percentage is higher than ACS, which indicate that the modified corn starch has better performance than native corn starch.
28522398	4	50	theme	substitution	866:877	arg1	solubility					844:853	solubility	844:853	solubility	844:853	These optimal conditions predicted by RSM were confirmed that the degree of substitution (DS) of acetate starch is 0.0691mg/g and the physical and chemical properties of natural corn starch (NCS) and corn starch acetate (ACS) were further studied.The transparency, water separation, water absorption, expansion force, and solubility of ACS low substitution are better than NCS, while the NCS's hydrolysis percentage is higher than ACS, which indicate that the modified corn starch has better performance than native corn starch.
28522398	4	50	theme	substitution	866:877	arg1	transparency					773:784	further studied.The transparency	753:784	further studied.The transparency	753:784	These optimal conditions predicted by RSM were confirmed that the degree of substitution (DS) of acetate starch is 0.0691mg/g and the physical and chemical properties of natural corn starch (NCS) and corn starch acetate (ACS) were further studied.The transparency, water separation, water absorption, expansion force, and solubility of ACS low substitution are better than NCS, while the NCS's hydrolysis percentage is higher than ACS, which indicate that the modified corn starch has better performance than native corn starch.
28522398	4	50	theme	substitution	866:877	arg1	separation					793:802	water separation	787:802	water separation	787:802	These optimal conditions predicted by RSM were confirmed that the degree of substitution (DS) of acetate starch is 0.0691mg/g and the physical and chemical properties of natural corn starch (NCS) and corn starch acetate (ACS) were further studied.The transparency, water separation, water absorption, expansion force, and solubility of ACS low substitution are better than NCS, while the NCS's hydrolysis percentage is higher than ACS, which indicate that the modified corn starch has better performance than native corn starch.
28522398	4	50	theme	substitution	866:877	arg1	absorption					811:820	water absorption	805:820	water absorption	805:820	These optimal conditions predicted by RSM were confirmed that the degree of substitution (DS) of acetate starch is 0.0691mg/g and the physical and chemical properties of natural corn starch (NCS) and corn starch acetate (ACS) were further studied.The transparency, water separation, water absorption, expansion force, and solubility of ACS low substitution are better than NCS, while the NCS's hydrolysis percentage is higher than ACS, which indicate that the modified corn starch has better performance than native corn starch.
28522398	4	50	theme	substitution	866:877	arg1	force					833:837	expansion force	823:837	expansion force	823:837	These optimal conditions predicted by RSM were confirmed that the degree of substitution (DS) of acetate starch is 0.0691mg/g and the physical and chemical properties of natural corn starch (NCS) and corn starch acetate (ACS) were further studied.The transparency, water separation, water absorption, expansion force, and solubility of ACS low substitution are better than NCS, while the NCS's hydrolysis percentage is higher than ACS, which indicate that the modified corn starch has better performance than native corn starch.
28522398	8	51	dep	Fourier	1449:1455	arg1	transform					1457:1465	transform	1457:1465	transform infrared (FTIR) spectroscopy	1457:1494	Fourier transform infrared (FTIR) spectroscopy shows that around 1750cm-1 carbonyl signal determines acetylation bonding successfully.
28522398	4	52	theme	further	753:759	arg1	transparency					773:784	further studied.The transparency	753:784	further studied.The transparency	753:784	These optimal conditions predicted by RSM were confirmed that the degree of substitution (DS) of acetate starch is 0.0691mg/g and the physical and chemical properties of natural corn starch (NCS) and corn starch acetate (ACS) were further studied.The transparency, water separation, water absorption, expansion force, and solubility of ACS low substitution are better than NCS, while the NCS's hydrolysis percentage is higher than ACS, which indicate that the modified corn starch has better performance than native corn starch.
28522398	0	53	with	synthesis	13:21	arg1	substitution					81:92	low substitution	77:92	low substitution under microwave assistance	77:119	Study on the synthesis and physicochemical properties of starch acetate with low substitution under microwave assistance.
28522398	3	54	theme	%	465:465	arg1	anhydride					450:458	acetic anhydride	443:458	acetic anhydride of 12%	443:465	The optimal parameters are as follows: amount of acetic anhydride of 12%, radiation time of 11min, and microwave power of 100W.
28522398	3	55	theme	11min	486:490	arg1	power					507:511	microwave power	497:511	microwave power of 100W	497:519	The optimal parameters are as follows: amount of acetic anhydride of 12%, radiation time of 11min, and microwave power of 100W.
28522398	3	55	theme	11min	486:490	arg1	anhydride					450:458	acetic anhydride	443:458	acetic anhydride of 12%	443:465	The optimal parameters are as follows: amount of acetic anhydride of 12%, radiation time of 11min, and microwave power of 100W.
28522398	3	55	theme	11min	486:490	arg1	time					478:481	radiation time	468:481	radiation time of 11min	468:490	The optimal parameters are as follows: amount of acetic anhydride of 12%, radiation time of 11min, and microwave power of 100W.
28522398	3	55	theme	11min	486:490	arg1	11min					486:490	11min	486:490	11min	486:490	The optimal parameters are as follows: amount of acetic anhydride of 12%, radiation time of 11min, and microwave power of 100W.
28522398	3	55	theme	11min	486:490	arg1	100W					516:519	100W	516:519	100W	516:519	The optimal parameters are as follows: amount of acetic anhydride of 12%, radiation time of 11min, and microwave power of 100W.
28522398	3	55	theme	11min	486:490	arg1	amount					433:438	amount	433:438	amount of acetic anhydride of 12%	433:465	The optimal parameters are as follows: amount of acetic anhydride of 12%, radiation time of 11min, and microwave power of 100W.
28522398	4	56	dep	better	883:888	arg1	were					748:751	were	748:751	were	748:751	These optimal conditions predicted by RSM were confirmed that the degree of substitution (DS) of acetate starch is 0.0691mg/g and the physical and chemical properties of natural corn starch (NCS) and corn starch acetate (ACS) were further studied.The transparency, water separation, water absorption, expansion force, and solubility of ACS low substitution are better than NCS, while the NCS's hydrolysis percentage is higher than ACS, which indicate that the modified corn starch has better performance than native corn starch.
28522398	4	57	theme	optimal	528:534	arg1	conditions					536:545	These optimal conditions	522:545	These optimal conditions predicted by RSM	522:562	These optimal conditions predicted by RSM were confirmed that the degree of substitution (DS) of acetate starch is 0.0691mg/g and the physical and chemical properties of natural corn starch (NCS) and corn starch acetate (ACS) were further studied.The transparency, water separation, water absorption, expansion force, and solubility of ACS low substitution are better than NCS, while the NCS's hydrolysis percentage is higher than ACS, which indicate that the modified corn starch has better performance than native corn starch.
28522398	1	58	with	acetate	188:194	arg1	substitution					205:216	low substitution	201:216	low substitution	201:216	In this study, synthesis and physicochemical properties of starch acetate with low substitution under microwave were studied.
28522398	4	59	theme	water	787:791	arg1	separation					793:802	water separation	787:802	water separation	787:802	These optimal conditions predicted by RSM were confirmed that the degree of substitution (DS) of acetate starch is 0.0691mg/g and the physical and chemical properties of natural corn starch (NCS) and corn starch acetate (ACS) were further studied.The transparency, water separation, water absorption, expansion force, and solubility of ACS low substitution are better than NCS, while the NCS's hydrolysis percentage is higher than ACS, which indicate that the modified corn starch has better performance than native corn starch.
28522398	5	60	theme	corn	1081:1084	arg1	acetate					1093:1099	the corn starch acetate	1077:1099	the corn starch acetate	1077:1099	The surface morphology of the corn starch acetate was examined by scanning electron microscope (SEM), which showed that it had a smooth surface and a spherical and polygonal shape.
28522398	0	61	dep	synthesis	13:21	arg1	the					9:11	the	9:11	the	9:11	Study on the synthesis and physicochemical properties of starch acetate with low substitution under microwave assistance.
28522398	2	62	theme	Composite	286:294	arg1	Design					296:301	A three-level-three-factorial Central Composite Design	248:301	A three-level-three-factorial Central Composite Design using Response Surface Methodology (RSM)	248:342	A three-level-three-factorial Central Composite Design using Response Surface Methodology (RSM) was employed to optimize the reaction conditions.
28522398	7	63	theme	Crystal	1270:1276	arg1	structure					1278:1286	Crystal structure	1270:1286	Crystal structure	1270:1286	Crystal structure was observed by X-ray diffraction, and the ACS can determine the level of microwave technology that can destroy the extent of the crystal and amorphous regions.
28522398	4	64	theme	corn	700:703	arg1	NCS					713:715	NCS	713:715	NCS	713:715	These optimal conditions predicted by RSM were confirmed that the degree of substitution (DS) of acetate starch is 0.0691mg/g and the physical and chemical properties of natural corn starch (NCS) and corn starch acetate (ACS) were further studied.The transparency, water separation, water absorption, expansion force, and solubility of ACS low substitution are better than NCS, while the NCS's hydrolysis percentage is higher than ACS, which indicate that the modified corn starch has better performance than native corn starch.
28522398	4	64	theme	corn	700:703	arg1	starch					705:710	natural corn starch	692:710	natural corn starch (NCS)	692:716	These optimal conditions predicted by RSM were confirmed that the degree of substitution (DS) of acetate starch is 0.0691mg/g and the physical and chemical properties of natural corn starch (NCS) and corn starch acetate (ACS) were further studied.The transparency, water separation, water absorption, expansion force, and solubility of ACS low substitution are better than NCS, while the NCS's hydrolysis percentage is higher than ACS, which indicate that the modified corn starch has better performance than native corn starch.
28522398	4	65	theme	corn	991:994	arg1	starch					996:1001	the modified corn starch	978:1001	the modified corn starch	978:1001	These optimal conditions predicted by RSM were confirmed that the degree of substitution (DS) of acetate starch is 0.0691mg/g and the physical and chemical properties of natural corn starch (NCS) and corn starch acetate (ACS) were further studied.The transparency, water separation, water absorption, expansion force, and solubility of ACS low substitution are better than NCS, while the NCS's hydrolysis percentage is higher than ACS, which indicate that the modified corn starch has better performance than native corn starch.
28522398	4	66	theme	acetate	734:740	arg1	better					883:888	better	883:888	better	883:888	These optimal conditions predicted by RSM were confirmed that the degree of substitution (DS) of acetate starch is 0.0691mg/g and the physical and chemical properties of natural corn starch (NCS) and corn starch acetate (ACS) were further studied.The transparency, water separation, water absorption, expansion force, and solubility of ACS low substitution are better than NCS, while the NCS's hydrolysis percentage is higher than ACS, which indicate that the modified corn starch has better performance than native corn starch.
28522398	4	66	theme	acetate	734:740	arg1	0.0691mg/g					637:646	0.0691mg/g	637:646	0.0691mg/g	637:646	These optimal conditions predicted by RSM were confirmed that the degree of substitution (DS) of acetate starch is 0.0691mg/g and the physical and chemical properties of natural corn starch (NCS) and corn starch acetate (ACS) were further studied.The transparency, water separation, water absorption, expansion force, and solubility of ACS low substitution are better than NCS, while the NCS's hydrolysis percentage is higher than ACS, which indicate that the modified corn starch has better performance than native corn starch.
28522398	4	66	theme	acetate	734:740	arg1	properties					678:687	the physical and chemical properties	652:687	the physical and chemical properties of natural corn starch (NCS) and corn starch acetate (ACS)	652:746	These optimal conditions predicted by RSM were confirmed that the degree of substitution (DS) of acetate starch is 0.0691mg/g and the physical and chemical properties of natural corn starch (NCS) and corn starch acetate (ACS) were further studied.The transparency, water separation, water absorption, expansion force, and solubility of ACS low substitution are better than NCS, while the NCS's hydrolysis percentage is higher than ACS, which indicate that the modified corn starch has better performance than native corn starch.
28522398	7	67	theme	technology	1372:1381	arg1	level					1353:1357	the level	1349:1357	the level of microwave technology that can destroy the extent of the crystal and amorphous regions	1349:1446	Crystal structure was observed by X-ray diffraction, and the ACS can determine the level of microwave technology that can destroy the extent of the crystal and amorphous regions.
28522398	2	68	theme	Central	278:284	arg1	Design					296:301	A three-level-three-factorial Central Composite Design	248:301	A three-level-three-factorial Central Composite Design using Response Surface Methodology (RSM)	248:342	A three-level-three-factorial Central Composite Design using Response Surface Methodology (RSM) was employed to optimize the reaction conditions.
28522398	4	69	theme	natural	692:698	arg1	NCS					713:715	NCS	713:715	NCS	713:715	These optimal conditions predicted by RSM were confirmed that the degree of substitution (DS) of acetate starch is 0.0691mg/g and the physical and chemical properties of natural corn starch (NCS) and corn starch acetate (ACS) were further studied.The transparency, water separation, water absorption, expansion force, and solubility of ACS low substitution are better than NCS, while the NCS's hydrolysis percentage is higher than ACS, which indicate that the modified corn starch has better performance than native corn starch.
28522398	4	69	theme	natural	692:698	arg1	starch					705:710	natural corn starch	692:710	natural corn starch (NCS)	692:716	These optimal conditions predicted by RSM were confirmed that the degree of substitution (DS) of acetate starch is 0.0691mg/g and the physical and chemical properties of natural corn starch (NCS) and corn starch acetate (ACS) were further studied.The transparency, water separation, water absorption, expansion force, and solubility of ACS low substitution are better than NCS, while the NCS's hydrolysis percentage is higher than ACS, which indicate that the modified corn starch has better performance than native corn starch.
28522398	4	70	theme	better	1007:1012	arg1	performance					1014:1024	better performance	1007:1024	better performance than native corn starch	1007:1048	These optimal conditions predicted by RSM were confirmed that the degree of substitution (DS) of acetate starch is 0.0691mg/g and the physical and chemical properties of natural corn starch (NCS) and corn starch acetate (ACS) were further studied.The transparency, water separation, water absorption, expansion force, and solubility of ACS low substitution are better than NCS, while the NCS's hydrolysis percentage is higher than ACS, which indicate that the modified corn starch has better performance than native corn starch.
28522398	0	71	theme	acetate	64:70	arg1	synthesis					13:21	synthesis	13:21	synthesis	13:21	Study on the synthesis and physicochemical properties of starch acetate with low substitution under microwave assistance.
28522398	0	71	theme	acetate	64:70	arg1	properties					43:52	physicochemical properties	27:52	physicochemical properties	27:52	Study on the synthesis and physicochemical properties of starch acetate with low substitution under microwave assistance.
28522398	4	72	dep	degree	588:593	arg1	is					634:635	is	634:635	is 0.0691mg/g and the physical and chemical properties of natural corn starch (NCS) and corn starch acetate (ACS) were further studied.The transparency, water separation, water absorption, expansion force, and solubility of ACS low substitution are better than NCS, while the NCS's hydrolysis percentage is higher than ACS, which indicate that the modified corn starch has better performance than native corn starch	634:1048	These optimal conditions predicted by RSM were confirmed that the degree of substitution (DS) of acetate starch is 0.0691mg/g and the physical and chemical properties of natural corn starch (NCS) and corn starch acetate (ACS) were further studied.The transparency, water separation, water absorption, expansion force, and solubility of ACS low substitution are better than NCS, while the NCS's hydrolysis percentage is higher than ACS, which indicate that the modified corn starch has better performance than native corn starch.
28522398	2	73	theme	three-level-three-factorial	250:276	arg1	Design					296:301	A three-level-three-factorial Central Composite Design	248:301	A three-level-three-factorial Central Composite Design using Response Surface Methodology (RSM)	248:342	A three-level-three-factorial Central Composite Design using Response Surface Methodology (RSM) was employed to optimize the reaction conditions.
28522398	0	74	with	properties	43:52	arg1	substitution					81:92	low substitution	77:92	low substitution under microwave assistance	77:119	Study on the synthesis and physicochemical properties of starch acetate with low substitution under microwave assistance.
28522398	4	75	theme	physical	656:663	arg1	better					883:888	better	883:888	better	883:888	These optimal conditions predicted by RSM were confirmed that the degree of substitution (DS) of acetate starch is 0.0691mg/g and the physical and chemical properties of natural corn starch (NCS) and corn starch acetate (ACS) were further studied.The transparency, water separation, water absorption, expansion force, and solubility of ACS low substitution are better than NCS, while the NCS's hydrolysis percentage is higher than ACS, which indicate that the modified corn starch has better performance than native corn starch.
28522398	4	75	theme	physical	656:663	arg1	properties					678:687	the physical and chemical properties	652:687	the physical and chemical properties of natural corn starch (NCS) and corn starch acetate (ACS)	652:746	These optimal conditions predicted by RSM were confirmed that the degree of substitution (DS) of acetate starch is 0.0691mg/g and the physical and chemical properties of natural corn starch (NCS) and corn starch acetate (ACS) were further studied.The transparency, water separation, water absorption, expansion force, and solubility of ACS low substitution are better than NCS, while the NCS's hydrolysis percentage is higher than ACS, which indicate that the modified corn starch has better performance than native corn starch.
28522398	8	76	theme	carbonyl	1523:1530	arg1	signal					1532:1537	around 1750cm-1 carbonyl signal	1507:1537	around 1750cm-1 carbonyl signal	1507:1537	Fourier transform infrared (FTIR) spectroscopy shows that around 1750cm-1 carbonyl signal determines acetylation bonding successfully.
28522398	0	77	theme	starch	57:62	arg1	acetate					64:70	starch acetate	57:70	starch acetate	57:70	Study on the synthesis and physicochemical properties of starch acetate with low substitution under microwave assistance.
28522398	5	78	theme	spherical	1201:1209	arg1	shape					1225:1229	a spherical and polygonal shape	1199:1229	shape	1225:1229	The surface morphology of the corn starch acetate was examined by scanning electron microscope (SEM), which showed that it had a smooth surface and a spherical and polygonal shape.
28522398	8	79	theme	1750cm-1	1514:1521	arg1	signal					1532:1537	around 1750cm-1 carbonyl signal	1507:1537	around 1750cm-1 carbonyl signal	1507:1537	Fourier transform infrared (FTIR) spectroscopy shows that around 1750cm-1 carbonyl signal determines acetylation bonding successfully.
28522398	4	80	theme	substitution	598:609	arg1	degree					588:593	the degree	584:593	the degree of substitution (DS) of acetate starch	584:632	These optimal conditions predicted by RSM were confirmed that the degree of substitution (DS) of acetate starch is 0.0691mg/g and the physical and chemical properties of natural corn starch (NCS) and corn starch acetate (ACS) were further studied.The transparency, water separation, water absorption, expansion force, and solubility of ACS low substitution are better than NCS, while the NCS's hydrolysis percentage is higher than ACS, which indicate that the modified corn starch has better performance than native corn starch.
28760354	4	0	theme	intact	572:577	arg1	n=8					598:600	n=8	598:600	n=8	598:600	To this end, quantitative T2 maps of histologically intact cartilage samples (n=8) were generated using a clinical 3.0-T MR imaging system.
28760354	4	0	theme	intact	572:577	arg1	samples					589:595	histologically intact cartilage samples	557:595	histologically intact cartilage samples (n=8)	557:601	To this end, quantitative T2 maps of histologically intact cartilage samples (n=8) were generated using a clinical 3.0-T MR imaging system.
28760354	10	1	theme	relaxation	1615:1624	arg1	times					1626:1630	T2 relaxation times	1612:1630	T2 relaxation times	1612:1630	On a pixel-wise basis, each individual component correlation with T2 relaxation times was determined by Spearman's ρs and significant correlations were found between T2 relaxation times and all four tissue parameters for all indentation strain levels.
28760354	11	2	with	techniques	1956:1965	arg1	regards					1972:1978	regards	1972:1978	regards to their perspective clinical applications	1972:2021	Thus, the biological changes in functional MR Imaging parameters such as T2 can further be characterized to strengthen the scientific basis of functional MRI techniques with regards to their perspective clinical applications.
28760354	3	3	theme	T2	508:509	arg1	mapping					511:517	quantitative T2 mapping	495:517	quantitative T2 mapping	495:517	As yet, however, it is not clear which cartilage component contributes to the tissue functionality as assessed by quantitative T2 mapping.
28760354	9	4	theme	collagen	1496:1503	arg1	content					1505:1511	collagen content	1496:1511	collagen content	1496:1511	These changes were parameterized as cartilage fluid, proteoglycan and collagen content as well as collagen orientation.
28760354	9	4	theme	collagen	1496:1503	arg1	fluid					1472:1476	cartilage fluid	1462:1476	cartilage fluid	1462:1476	These changes were parameterized as cartilage fluid, proteoglycan and collagen content as well as collagen orientation.
28760354	4	5	theme	samples	589:595	arg1	maps					549:552	quantitative T2 maps	533:552	quantitative T2 maps of histologically intact cartilage samples (n=8)	533:601	To this end, quantitative T2 maps of histologically intact cartilage samples (n=8) were generated using a clinical 3.0-T MR imaging system.
28760354	3	6	theme	quantitative	495:506	arg1	mapping					511:517	quantitative T2 mapping	495:517	quantitative T2 mapping	495:517	As yet, however, it is not clear which cartilage component contributes to the tissue functionality as assessed by quantitative T2 mapping.
28760354	6	7	theme	unconfined	930:939	arg1	compression					941:951	unconfined compression	930:951	unconfined compression	930:951	Samples underwent conventional biomechanical testing (by unconfined compression) as well as histological assessment (by Mankin scoring) for reference purposes.
28760354	4	8	theme	MR	641:642	arg1	system					652:657	a clinical 3.0-T MR imaging system	624:657	a clinical 3.0-T MR imaging system	624:657	To this end, quantitative T2 maps of histologically intact cartilage samples (n=8) were generated using a clinical 3.0-T MR imaging system.
28760354	2	9	theme	magnetic	287:294	arg1	MR					307:308	MR	307:308	MR	307:308	One promising non-invasive approach is to functionally assess the tissue response to loading by serial magnetic resonance (MR) imaging in terms of T2 mapping under simultaneous mechanical loading.
28760354	2	9	theme	magnetic	287:294	arg1	resonance					296:304	serial magnetic resonance	280:304	serial magnetic resonance (MR) imaging in terms of T2 mapping under simultaneous mechanical loading	280:378	One promising non-invasive approach is to functionally assess the tissue response to loading by serial magnetic resonance (MR) imaging in terms of T2 mapping under simultaneous mechanical loading.
28760354	8	10	theme	intra-tissue	1243:1254	arg1	changes					1256:1262	compositional and structural intra-tissue changes	1214:1262	changes	1256:1262	In efforts to simulate the evolution of compositional and structural intra-tissue changes under quasi-static loading, the indentation-induced changes in quantitative T2 maps were referenced to underlying changes in cartilage composition and structure.
28760354	8	11	theme	quantitative	1327:1338	arg1	maps					1343:1346	quantitative T2 maps	1327:1346	quantitative T2 maps	1327:1346	In efforts to simulate the evolution of compositional and structural intra-tissue changes under quasi-static loading, the indentation-induced changes in quantitative T2 maps were referenced to underlying changes in cartilage composition and structure.
28760354	2	12	theme	simultaneous	348:359	arg1	loading					372:378	simultaneous mechanical loading	348:378	simultaneous mechanical loading	348:378	One promising non-invasive approach is to functionally assess the tissue response to loading by serial magnetic resonance (MR) imaging in terms of T2 mapping under simultaneous mechanical loading.
28760354	6	13	theme	Mankin	993:998	arg1	scoring					1000:1006	Mankin scoring	993:1006	Mankin scoring	993:1006	Samples underwent conventional biomechanical testing (by unconfined compression) as well as histological assessment (by Mankin scoring) for reference purposes.
28760354	4	14	theme	clinical	626:633	arg1	system					652:657	a clinical 3.0-T MR imaging system	624:657	a clinical 3.0-T MR imaging system	624:657	To this end, quantitative T2 maps of histologically intact cartilage samples (n=8) were generated using a clinical 3.0-T MR imaging system.
28760354	7	15	dep	element	1133:1139	arg1	code					1146:1149	code	1146:1149	code for cross-referencing	1146:1171	Moreover, an anisotropic hyperelastic constitutive model of cartilage was implemented into a finite element (FE) code for cross-referencing.
28760354	5	16	theme	serial	724:729	arg1	mapping					734:740	serial T2 mapping	724:740	serial T2 mapping	724:740	Using displacement-controlled quasi-static indentation loading, serial T2 mapping was performed at three defined strain levels and loading-induced relative changes were determined in distinct regions-of-interest.
28760354	2	17	theme	tissue	250:255	arg1	response					257:264	the tissue response	246:264	the tissue response to loading	246:275	One promising non-invasive approach is to functionally assess the tissue response to loading by serial magnetic resonance (MR) imaging in terms of T2 mapping under simultaneous mechanical loading.
28760354	8	18	from	changes	1316:1322	arg1	maps					1343:1346	quantitative T2 maps	1327:1346	quantitative T2 maps	1327:1346	In efforts to simulate the evolution of compositional and structural intra-tissue changes under quasi-static loading, the indentation-induced changes in quantitative T2 maps were referenced to underlying changes in cartilage composition and structure.
28760354	11	19	from	changes	1819:1825	arg1	parameters					1852:1861	functional MR Imaging parameters	1830:1861	functional MR Imaging parameters such as T2	1830:1872	Thus, the biological changes in functional MR Imaging parameters such as T2 can further be characterized to strengthen the scientific basis of functional MRI techniques with regards to their perspective clinical applications.
28760354	11	19	from	changes	1819:1825	arg1	T2					1871:1872	T2	1871:1872	T2	1871:1872	Thus, the biological changes in functional MR Imaging parameters such as T2 can further be characterized to strengthen the scientific basis of functional MRI techniques with regards to their perspective clinical applications.
28760354	5	20	theme	displacement-controlled	666:688	arg1	loading					715:721	displacement-controlled quasi-static indentation loading	666:721	displacement-controlled quasi-static indentation loading	666:721	Using displacement-controlled quasi-static indentation loading, serial T2 mapping was performed at three defined strain levels and loading-induced relative changes were determined in distinct regions-of-interest.
28760354	7	21	theme	cartilage	1093:1101	arg1	model					1084:1088	an anisotropic hyperelastic constitutive model	1043:1088	an anisotropic hyperelastic constitutive model of cartilage	1043:1101	Moreover, an anisotropic hyperelastic constitutive model of cartilage was implemented into a finite element (FE) code for cross-referencing.
28760354	1	22	theme	stages	115:120	arg1	detection					96:104	The detection	92:104	The detection of early stages of cartilage degeneration	92:146	The detection of early stages of cartilage degeneration remains diagnostically challenging.
28760354	6	23	theme	histological	965:976	arg1	assessment					978:987	histological assessment	965:987	conventional biomechanical testing (by unconfined compression) as well as histological assessment (by Mankin scoring)	891:1007	Samples underwent conventional biomechanical testing (by unconfined compression) as well as histological assessment (by Mankin scoring) for reference purposes.
28760354	8	24	theme	underlying	1367:1376	arg1	changes					1378:1384	underlying changes	1367:1384	underlying changes in cartilage composition and structure	1367:1423	In efforts to simulate the evolution of compositional and structural intra-tissue changes under quasi-static loading, the indentation-induced changes in quantitative T2 maps were referenced to underlying changes in cartilage composition and structure.
28760354	11	25	theme	functional	1830:1839	arg1	Imaging					1844:1850	functional MR Imaging	1830:1850	functional MR Imaging parameters such as T2	1830:1872	Thus, the biological changes in functional MR Imaging parameters such as T2 can further be characterized to strengthen the scientific basis of functional MRI techniques with regards to their perspective clinical applications.
28760354	5	26	theme	quasi-static	690:701	arg1	loading					715:721	displacement-controlled quasi-static indentation loading	666:721	displacement-controlled quasi-static indentation loading	666:721	Using displacement-controlled quasi-static indentation loading, serial T2 mapping was performed at three defined strain levels and loading-induced relative changes were determined in distinct regions-of-interest.
28760354	1	27	theme	cartilage	125:133	arg1	degeneration					135:146	cartilage degeneration	125:146	cartilage degeneration	125:146	The detection of early stages of cartilage degeneration remains diagnostically challenging.
28760354	0	28	theme	articular	50:58	arg1	cartilage					60:68	human articular cartilage	44:68	human articular cartilage	44:68	T2 MR imaging vs. computational modeling of human articular cartilage tissue functionality.
28760354	11	29	theme	scientific	1921:1930	arg1	basis					1932:1936	the scientific basis	1917:1936	the scientific basis of functional MRI techniques with regards to their perspective clinical applications	1917:2021	Thus, the biological changes in functional MR Imaging parameters such as T2 can further be characterized to strengthen the scientific basis of functional MRI techniques with regards to their perspective clinical applications.
28760354	10	30	with	correlation	1595:1605	arg1	times					1626:1630	T2 relaxation times	1612:1630	T2 relaxation times	1612:1630	On a pixel-wise basis, each individual component correlation with T2 relaxation times was determined by Spearman's ρs and significant correlations were found between T2 relaxation times and all four tissue parameters for all indentation strain levels.
28760354	5	31	theme	strain	773:778	arg1	levels					780:785	three defined strain levels	759:785	three defined strain levels	759:785	Using displacement-controlled quasi-static indentation loading, serial T2 mapping was performed at three defined strain levels and loading-induced relative changes were determined in distinct regions-of-interest.
28760354	11	32	theme	Imaging	1844:1850	arg1	parameters					1852:1861	functional MR Imaging parameters	1830:1861	functional MR Imaging parameters such as T2	1830:1872	Thus, the biological changes in functional MR Imaging parameters such as T2 can further be characterized to strengthen the scientific basis of functional MRI techniques with regards to their perspective clinical applications.
28760354	11	32	theme	Imaging	1844:1850	arg1	T2					1871:1872	T2	1871:1872	T2	1871:1872	Thus, the biological changes in functional MR Imaging parameters such as T2 can further be characterized to strengthen the scientific basis of functional MRI techniques with regards to their perspective clinical applications.
28760354	6	33	theme	reference	1013:1021	arg1	purposes					1023:1030	reference purposes	1013:1030	reference purposes	1013:1030	Samples underwent conventional biomechanical testing (by unconfined compression) as well as histological assessment (by Mankin scoring) for reference purposes.
28760354	10	34	theme	pixel-wise	1551:1560	arg1	basis					1562:1566	a pixel-wise basis	1549:1566	a pixel-wise basis	1549:1566	On a pixel-wise basis, each individual component correlation with T2 relaxation times was determined by Spearman's ρs and significant correlations were found between T2 relaxation times and all four tissue parameters for all indentation strain levels.
28760354	3	35	theme	cartilage	420:428	arg1	component					430:438	component	430:438	component	430:438	As yet, however, it is not clear which cartilage component contributes to the tissue functionality as assessed by quantitative T2 mapping.
28760354	0	36	theme	T2	0:1	arg1	imaging					6:12	T2 MR imaging	0:12	T2 MR imaging	0:12	T2 MR imaging vs. computational modeling of human articular cartilage tissue functionality.
28760354	4	37	theme	quantitative	533:544	arg1	maps					549:552	quantitative T2 maps	533:552	quantitative T2 maps of histologically intact cartilage samples (n=8)	533:601	To this end, quantitative T2 maps of histologically intact cartilage samples (n=8) were generated using a clinical 3.0-T MR imaging system.
28760354	10	38	theme	individual	1574:1583	arg1	correlation					1595:1605	each individual component correlation	1569:1605	each individual component correlation with T2 relaxation times	1569:1630	On a pixel-wise basis, each individual component correlation with T2 relaxation times was determined by Spearman's ρs and significant correlations were found between T2 relaxation times and all four tissue parameters for all indentation strain levels.
28760354	2	39	theme	promising	188:196	arg1	approach					211:218	One promising non-invasive approach	184:218	One promising non-invasive approach	184:218	One promising non-invasive approach is to functionally assess the tissue response to loading by serial magnetic resonance (MR) imaging in terms of T2 mapping under simultaneous mechanical loading.
28760354	0	40	theme	computational	18:30	arg1	modeling					32:39	computational modeling	18:39	computational modeling	18:39	T2 MR imaging vs. computational modeling of human articular cartilage tissue functionality.
28760354	6	41	theme	conventional	891:902	arg1	testing					918:924	conventional biomechanical testing	891:924	conventional biomechanical testing (by unconfined compression) as well as histological assessment (by Mankin scoring)	891:1007	Samples underwent conventional biomechanical testing (by unconfined compression) as well as histological assessment (by Mankin scoring) for reference purposes.
28760354	10	42	theme	T2	1612:1613	arg1	times					1626:1630	T2 relaxation times	1612:1630	T2 relaxation times	1612:1630	On a pixel-wise basis, each individual component correlation with T2 relaxation times was determined by Spearman's ρs and significant correlations were found between T2 relaxation times and all four tissue parameters for all indentation strain levels.
28760354	5	43	theme	relative	807:814	arg1	changes					816:822	loading-induced relative changes	791:822	loading-induced relative changes	791:822	Using displacement-controlled quasi-static indentation loading, serial T2 mapping was performed at three defined strain levels and loading-induced relative changes were determined in distinct regions-of-interest.
28760354	11	44	theme	biological	1808:1817	arg1	changes					1819:1825	the biological changes	1804:1825	the biological changes in functional MR Imaging parameters such as T2	1804:1872	Thus, the biological changes in functional MR Imaging parameters such as T2 can further be characterized to strengthen the scientific basis of functional MRI techniques with regards to their perspective clinical applications.
28760354	11	45	theme	MRI	1952:1954	arg1	techniques					1956:1965	functional MRI techniques	1941:1965	functional MRI techniques with regards to their perspective clinical applications	1941:2021	Thus, the biological changes in functional MR Imaging parameters such as T2 can further be characterized to strengthen the scientific basis of functional MRI techniques with regards to their perspective clinical applications.
28760354	10	46	theme	strain	1783:1788	arg1	levels					1790:1795	all indentation strain levels	1767:1795	all indentation strain levels	1767:1795	On a pixel-wise basis, each individual component correlation with T2 relaxation times was determined by Spearman's ρs and significant correlations were found between T2 relaxation times and all four tissue parameters for all indentation strain levels.
28760354	9	47	theme	cartilage	1462:1470	arg1	proteoglycan					1479:1490	proteoglycan	1479:1490	proteoglycan	1479:1490	These changes were parameterized as cartilage fluid, proteoglycan and collagen content as well as collagen orientation.
28760354	9	47	theme	cartilage	1462:1470	arg1	orientation					1533:1543	collagen orientation	1524:1543	proteoglycan and collagen content as well as collagen orientation	1479:1543	These changes were parameterized as cartilage fluid, proteoglycan and collagen content as well as collagen orientation.
28760354	9	47	theme	cartilage	1462:1470	arg1	content					1505:1511	collagen content	1496:1511	collagen content	1496:1511	These changes were parameterized as cartilage fluid, proteoglycan and collagen content as well as collagen orientation.
28760354	9	47	theme	cartilage	1462:1470	arg1	fluid					1472:1476	cartilage fluid	1462:1476	cartilage fluid	1462:1476	These changes were parameterized as cartilage fluid, proteoglycan and collagen content as well as collagen orientation.
28760354	9	47	theme	cartilage	1462:1470	arg1	changes					1432:1438	These changes	1426:1438	These changes	1426:1438	These changes were parameterized as cartilage fluid, proteoglycan and collagen content as well as collagen orientation.
28760354	0	48	theme	human	44:48	arg1	cartilage					60:68	human articular cartilage	44:68	human articular cartilage	44:68	T2 MR imaging vs. computational modeling of human articular cartilage tissue functionality.
28760354	2	49	theme	mechanical	361:370	arg1	loading					372:378	simultaneous mechanical loading	348:378	simultaneous mechanical loading	348:378	One promising non-invasive approach is to functionally assess the tissue response to loading by serial magnetic resonance (MR) imaging in terms of T2 mapping under simultaneous mechanical loading.
28760354	2	50	theme	T2	331:332	arg1	mapping					334:340	T2 mapping	331:340	T2 mapping	331:340	One promising non-invasive approach is to functionally assess the tissue response to loading by serial magnetic resonance (MR) imaging in terms of T2 mapping under simultaneous mechanical loading.
28760354	4	51	theme	cartilage	579:587	arg1	n=8					598:600	n=8	598:600	n=8	598:600	To this end, quantitative T2 maps of histologically intact cartilage samples (n=8) were generated using a clinical 3.0-T MR imaging system.
28760354	4	51	theme	cartilage	579:587	arg1	samples					589:595	histologically intact cartilage samples	557:595	histologically intact cartilage samples (n=8)	557:601	To this end, quantitative T2 maps of histologically intact cartilage samples (n=8) were generated using a clinical 3.0-T MR imaging system.
28760354	4	52	theme	3.0-T	635:639	arg1	system					652:657	a clinical 3.0-T MR imaging system	624:657	a clinical 3.0-T MR imaging system	624:657	To this end, quantitative T2 maps of histologically intact cartilage samples (n=8) were generated using a clinical 3.0-T MR imaging system.
28760354	7	53	theme	finite	1126:1131	arg1	FE					1142:1143	FE	1142:1143	FE	1142:1143	Moreover, an anisotropic hyperelastic constitutive model of cartilage was implemented into a finite element (FE) code for cross-referencing.
28760354	7	53	theme	finite	1126:1131	arg1	element					1133:1139	a finite element	1124:1139	a finite element (FE) code for cross-referencing	1124:1171	Moreover, an anisotropic hyperelastic constitutive model of cartilage was implemented into a finite element (FE) code for cross-referencing.
28760354	9	54	theme	collagen	1524:1531	arg1	orientation					1533:1543	collagen orientation	1524:1543	proteoglycan and collagen content as well as collagen orientation	1479:1543	These changes were parameterized as cartilage fluid, proteoglycan and collagen content as well as collagen orientation.
28760354	9	54	theme	collagen	1524:1531	arg1	fluid					1472:1476	cartilage fluid	1462:1476	cartilage fluid	1462:1476	These changes were parameterized as cartilage fluid, proteoglycan and collagen content as well as collagen orientation.
28760354	8	55	theme	compositional	1214:1226	arg1	changes					1256:1262	compositional and structural intra-tissue changes	1214:1262	changes	1256:1262	In efforts to simulate the evolution of compositional and structural intra-tissue changes under quasi-static loading, the indentation-induced changes in quantitative T2 maps were referenced to underlying changes in cartilage composition and structure.
28760354	4	56	theme	imaging	644:650	arg1	system					652:657	a clinical 3.0-T MR imaging system	624:657	a clinical 3.0-T MR imaging system	624:657	To this end, quantitative T2 maps of histologically intact cartilage samples (n=8) were generated using a clinical 3.0-T MR imaging system.
28760354	10	57	theme	significant	1668:1678	arg1	correlations					1680:1691	significant correlations	1668:1691	significant correlations	1668:1691	On a pixel-wise basis, each individual component correlation with T2 relaxation times was determined by Spearman's ρs and significant correlations were found between T2 relaxation times and all four tissue parameters for all indentation strain levels.
28760354	5	58	theme	indentation	703:713	arg1	loading					715:721	displacement-controlled quasi-static indentation loading	666:721	displacement-controlled quasi-static indentation loading	666:721	Using displacement-controlled quasi-static indentation loading, serial T2 mapping was performed at three defined strain levels and loading-induced relative changes were determined in distinct regions-of-interest.
28760354	5	59	theme	distinct	843:850	arg1	regions-of-interest					852:870	distinct regions-of-interest	843:870	distinct regions-of-interest	843:870	Using displacement-controlled quasi-static indentation loading, serial T2 mapping was performed at three defined strain levels and loading-induced relative changes were determined in distinct regions-of-interest.
28760354	8	60	theme	structural	1232:1241	arg1	changes					1256:1262	compositional and structural intra-tissue changes	1214:1262	changes	1256:1262	In efforts to simulate the evolution of compositional and structural intra-tissue changes under quasi-static loading, the indentation-induced changes in quantitative T2 maps were referenced to underlying changes in cartilage composition and structure.
28760354	10	61	theme	relaxation	1715:1724	arg1	times					1726:1730	T2 relaxation times	1712:1730	T2 relaxation times	1712:1730	On a pixel-wise basis, each individual component correlation with T2 relaxation times was determined by Spearman's ρs and significant correlations were found between T2 relaxation times and all four tissue parameters for all indentation strain levels.
28760354	11	62	theme	functional	1941:1950	arg1	techniques					1956:1965	functional MRI techniques	1941:1965	functional MRI techniques with regards to their perspective clinical applications	1941:2021	Thus, the biological changes in functional MR Imaging parameters such as T2 can further be characterized to strengthen the scientific basis of functional MRI techniques with regards to their perspective clinical applications.
28760354	8	63	theme	indentation-induced	1296:1314	arg1	changes					1316:1322	the indentation-induced changes	1292:1322	the indentation-induced changes in quantitative T2 maps	1292:1346	In efforts to simulate the evolution of compositional and structural intra-tissue changes under quasi-static loading, the indentation-induced changes in quantitative T2 maps were referenced to underlying changes in cartilage composition and structure.
28760354	8	64	theme	changes	1256:1262	arg1	evolution					1201:1209	the evolution	1197:1209	the evolution of compositional and structural intra-tissue changes under quasi-static loading	1197:1289	In efforts to simulate the evolution of compositional and structural intra-tissue changes under quasi-static loading, the indentation-induced changes in quantitative T2 maps were referenced to underlying changes in cartilage composition and structure.
28760354	8	65	theme	T2	1340:1341	arg1	maps					1343:1346	quantitative T2 maps	1327:1346	quantitative T2 maps	1327:1346	In efforts to simulate the evolution of compositional and structural intra-tissue changes under quasi-static loading, the indentation-induced changes in quantitative T2 maps were referenced to underlying changes in cartilage composition and structure.
28760354	7	66	theme	hyperelastic	1058:1069	arg1	model					1084:1088	an anisotropic hyperelastic constitutive model	1043:1088	an anisotropic hyperelastic constitutive model of cartilage	1043:1101	Moreover, an anisotropic hyperelastic constitutive model of cartilage was implemented into a finite element (FE) code for cross-referencing.
28760354	1	67	theme	early	109:113	arg1	stages					115:120	early stages	109:120	early stages of cartilage degeneration	109:146	The detection of early stages of cartilage degeneration remains diagnostically challenging.
28760354	8	68	theme	quasi-static	1270:1281	arg1	loading					1283:1289	quasi-static loading	1270:1289	quasi-static loading	1270:1289	In efforts to simulate the evolution of compositional and structural intra-tissue changes under quasi-static loading, the indentation-induced changes in quantitative T2 maps were referenced to underlying changes in cartilage composition and structure.
28760354	7	69	theme	constitutive	1071:1082	arg1	model					1084:1088	an anisotropic hyperelastic constitutive model	1043:1088	an anisotropic hyperelastic constitutive model of cartilage	1043:1101	Moreover, an anisotropic hyperelastic constitutive model of cartilage was implemented into a finite element (FE) code for cross-referencing.
28760354	2	70	theme	serial	280:285	arg1	MR					307:308	MR	307:308	MR	307:308	One promising non-invasive approach is to functionally assess the tissue response to loading by serial magnetic resonance (MR) imaging in terms of T2 mapping under simultaneous mechanical loading.
28760354	2	70	theme	serial	280:285	arg1	resonance					296:304	serial magnetic resonance	280:304	serial magnetic resonance (MR) imaging in terms of T2 mapping under simultaneous mechanical loading	280:378	One promising non-invasive approach is to functionally assess the tissue response to loading by serial magnetic resonance (MR) imaging in terms of T2 mapping under simultaneous mechanical loading.
28760354	11	71	theme	MR	1841:1842	arg1	Imaging					1844:1850	functional MR Imaging	1830:1850	functional MR Imaging parameters such as T2	1830:1872	Thus, the biological changes in functional MR Imaging parameters such as T2 can further be characterized to strengthen the scientific basis of functional MRI techniques with regards to their perspective clinical applications.
28760354	0	72	theme	cartilage	60:68	arg1	imaging					6:12	T2 MR imaging	0:12	T2 MR imaging	0:12	T2 MR imaging vs. computational modeling of human articular cartilage tissue functionality.
28760354	1	73	theme	degeneration	135:146	arg1	stages					115:120	early stages	109:120	early stages of cartilage degeneration	109:146	The detection of early stages of cartilage degeneration remains diagnostically challenging.
28760354	5	74	theme	defined	765:771	arg1	levels					780:785	three defined strain levels	759:785	three defined strain levels	759:785	Using displacement-controlled quasi-static indentation loading, serial T2 mapping was performed at three defined strain levels and loading-induced relative changes were determined in distinct regions-of-interest.
28760354	11	75	theme	techniques	1956:1965	arg1	basis					1932:1936	the scientific basis	1917:1936	the scientific basis of functional MRI techniques with regards to their perspective clinical applications	1917:2021	Thus, the biological changes in functional MR Imaging parameters such as T2 can further be characterized to strengthen the scientific basis of functional MRI techniques with regards to their perspective clinical applications.
28760354	8	76	theme	cartilage	1389:1397	arg1	composition					1399:1409	cartilage composition	1389:1409	cartilage composition	1389:1409	In efforts to simulate the evolution of compositional and structural intra-tissue changes under quasi-static loading, the indentation-induced changes in quantitative T2 maps were referenced to underlying changes in cartilage composition and structure.
28760354	5	77	theme	T2	731:732	arg1	mapping					734:740	serial T2 mapping	724:740	serial T2 mapping	724:740	Using displacement-controlled quasi-static indentation loading, serial T2 mapping was performed at three defined strain levels and loading-induced relative changes were determined in distinct regions-of-interest.
28760354	8	78	dep	simulate	1188:1195	arg1	efforts					1177:1183	efforts	1177:1183	efforts	1177:1183	In efforts to simulate the evolution of compositional and structural intra-tissue changes under quasi-static loading, the indentation-induced changes in quantitative T2 maps were referenced to underlying changes in cartilage composition and structure.
28760354	11	79	theme	clinical	2001:2008	arg1	applications					2010:2021	clinical applications	2001:2021	clinical applications	2001:2021	Thus, the biological changes in functional MR Imaging parameters such as T2 can further be characterized to strengthen the scientific basis of functional MRI techniques with regards to their perspective clinical applications.
28760354	0	80	theme	MR	3:4	arg1	imaging					6:12	T2 MR imaging	0:12	T2 MR imaging	0:12	T2 MR imaging vs. computational modeling of human articular cartilage tissue functionality.
28760354	4	81	theme	T2	546:547	arg1	maps					549:552	quantitative T2 maps	533:552	quantitative T2 maps of histologically intact cartilage samples (n=8)	533:601	To this end, quantitative T2 maps of histologically intact cartilage samples (n=8) were generated using a clinical 3.0-T MR imaging system.
28760354	8	82	from	changes	1378:1384	arg1	structure					1415:1423	structure	1415:1423	structure	1415:1423	In efforts to simulate the evolution of compositional and structural intra-tissue changes under quasi-static loading, the indentation-induced changes in quantitative T2 maps were referenced to underlying changes in cartilage composition and structure.
28760354	8	82	from	changes	1378:1384	arg1	composition					1399:1409	cartilage composition	1389:1409	cartilage composition	1389:1409	In efforts to simulate the evolution of compositional and structural intra-tissue changes under quasi-static loading, the indentation-induced changes in quantitative T2 maps were referenced to underlying changes in cartilage composition and structure.
28760354	2	83	theme	non-invasive	198:209	arg1	approach					211:218	One promising non-invasive approach	184:218	One promising non-invasive approach	184:218	One promising non-invasive approach is to functionally assess the tissue response to loading by serial magnetic resonance (MR) imaging in terms of T2 mapping under simultaneous mechanical loading.
28760354	3	84	theme	tissue	459:464	arg1	functionality					466:478	the tissue functionality	455:478	the tissue functionality	455:478	As yet, however, it is not clear which cartilage component contributes to the tissue functionality as assessed by quantitative T2 mapping.
28760354	6	85	theme	biomechanical	904:916	arg1	testing					918:924	conventional biomechanical testing	891:924	conventional biomechanical testing (by unconfined compression) as well as histological assessment (by Mankin scoring)	891:1007	Samples underwent conventional biomechanical testing (by unconfined compression) as well as histological assessment (by Mankin scoring) for reference purposes.
28760354	10	86	theme	T2	1712:1713	arg1	times					1726:1730	T2 relaxation times	1712:1730	T2 relaxation times	1712:1730	On a pixel-wise basis, each individual component correlation with T2 relaxation times was determined by Spearman's ρs and significant correlations were found between T2 relaxation times and all four tissue parameters for all indentation strain levels.
28760354	10	87	theme	tissue	1745:1750	arg1	parameters					1752:1761	all four tissue parameters	1736:1761	all four tissue parameters	1736:1761	On a pixel-wise basis, each individual component correlation with T2 relaxation times was determined by Spearman's ρs and significant correlations were found between T2 relaxation times and all four tissue parameters for all indentation strain levels.
28760354	5	88	theme	loading-induced	791:805	arg1	changes					816:822	loading-induced relative changes	791:822	loading-induced relative changes	791:822	Using displacement-controlled quasi-static indentation loading, serial T2 mapping was performed at three defined strain levels and loading-induced relative changes were determined in distinct regions-of-interest.
28760354	11	89	dep	perspective	1989:1999	arg1	applications					2010:2021	clinical applications	2001:2021	clinical applications	2001:2021	Thus, the biological changes in functional MR Imaging parameters such as T2 can further be characterized to strengthen the scientific basis of functional MRI techniques with regards to their perspective clinical applications.
28760354	2	90	theme	mapping	334:340	arg1	terms					322:326	terms	322:326	terms of T2 mapping	322:340	One promising non-invasive approach is to functionally assess the tissue response to loading by serial magnetic resonance (MR) imaging in terms of T2 mapping under simultaneous mechanical loading.
28760354	10	91	theme	component	1585:1593	arg1	correlation					1595:1605	each individual component correlation	1569:1605	each individual component correlation with T2 relaxation times	1569:1630	On a pixel-wise basis, each individual component correlation with T2 relaxation times was determined by Spearman's ρs and significant correlations were found between T2 relaxation times and all four tissue parameters for all indentation strain levels.
28760354	7	92	theme	anisotropic	1046:1056	arg1	model					1084:1088	an anisotropic hyperelastic constitutive model	1043:1088	an anisotropic hyperelastic constitutive model of cartilage	1043:1101	Moreover, an anisotropic hyperelastic constitutive model of cartilage was implemented into a finite element (FE) code for cross-referencing.
28760354	10	93	theme	indentation	1771:1781	arg1	levels					1790:1795	all indentation strain levels	1767:1795	all indentation strain levels	1767:1795	On a pixel-wise basis, each individual component correlation with T2 relaxation times was determined by Spearman's ρs and significant correlations were found between T2 relaxation times and all four tissue parameters for all indentation strain levels.
28155165	3	0	from	lipids	502:507	arg1	structures					561:570	the secretory structures	547:570	the secretory structures	547:570	Here I describe some of the most used methods to detect carbohydrates, proteins, lipids, phenolic compounds, and alkaloids in the secretory structures.
28155165	2	1	theme	substances	271:280	arg1	types					262:266	one or more types	250:266	one or more types of substances	250:280	As each gland may produce one or more types of substances, a correct analysis of its secretion should be done using various histochemical tests to detect metabolites of different chemical classes.
28155165	3	2	from	carbohydrates	477:489	arg1	structures					561:570	the secretory structures	547:570	the secretory structures	547:570	Here I describe some of the most used methods to detect carbohydrates, proteins, lipids, phenolic compounds, and alkaloids in the secretory structures.
28155165	1	3	theme	secretory	113:121	arg1	structures					123:132	plant secretory structures	107:132	plant secretory structures whose classification is based, at least partially, on the composition of their secretion	107:221	Histochemical analysis is essential for the study of plant secretory structures whose classification is based, at least partially, on the composition of their secretion.
28155165	2	4	theme	correct	285:291	arg1	analysis					293:300	a correct analysis	283:300	a correct analysis of its secretion	283:317	As each gland may produce one or more types of substances, a correct analysis of its secretion should be done using various histochemical tests to detect metabolites of different chemical classes.
28155165	2	5	theme	histochemical	348:360	arg1	tests					362:366	various histochemical tests	340:366	various histochemical tests	340:366	As each gland may produce one or more types of substances, a correct analysis of its secretion should be done using various histochemical tests to detect metabolites of different chemical classes.
28155165	0	6	theme	Histochemical	0:12	arg1	Analysis					14:21	Histochemical Analysis	0:21	Histochemical Analysis of Plant Secretory Structures	0:51	Histochemical Analysis of Plant Secretory Structures.
28155165	0	7	theme	Plant	26:30	arg1	Structures					42:51	Plant Secretory Structures	26:51	Plant Secretory Structures	26:51	Histochemical Analysis of Plant Secretory Structures.
28155165	3	8	from	alkaloids	534:542	arg1	structures					561:570	the secretory structures	547:570	the secretory structures	547:570	Here I describe some of the most used methods to detect carbohydrates, proteins, lipids, phenolic compounds, and alkaloids in the secretory structures.
28155165	2	9	theme	various	340:346	arg1	tests					362:366	various histochemical tests	340:366	various histochemical tests	340:366	As each gland may produce one or more types of substances, a correct analysis of its secretion should be done using various histochemical tests to detect metabolites of different chemical classes.
28155165	2	10	theme	classes	412:418	arg1	metabolites					378:388	metabolites	378:388	metabolites of different chemical classes	378:418	As each gland may produce one or more types of substances, a correct analysis of its secretion should be done using various histochemical tests to detect metabolites of different chemical classes.
28155165	3	11	theme	phenolic	510:517	arg1	compounds					519:527	phenolic compounds	510:527	phenolic compounds	510:527	Here I describe some of the most used methods to detect carbohydrates, proteins, lipids, phenolic compounds, and alkaloids in the secretory structures.
28155165	2	12	theme	different	393:401	arg1	classes					412:418	different chemical classes	393:418	different chemical classes	393:418	As each gland may produce one or more types of substances, a correct analysis of its secretion should be done using various histochemical tests to detect metabolites of different chemical classes.
28155165	2	13	theme	chemical	403:410	arg1	classes					412:418	different chemical classes	393:418	different chemical classes	393:418	As each gland may produce one or more types of substances, a correct analysis of its secretion should be done using various histochemical tests to detect metabolites of different chemical classes.
28155165	1	14	theme	plant	107:111	arg1	structures					123:132	plant secretory structures	107:132	plant secretory structures whose classification is based, at least partially, on the composition of their secretion	107:221	Histochemical analysis is essential for the study of plant secretory structures whose classification is based, at least partially, on the composition of their secretion.
28155165	0	15	theme	Structures	42:51	arg1	Analysis					14:21	Histochemical Analysis	0:21	Histochemical Analysis of Plant Secretory Structures	0:51	Histochemical Analysis of Plant Secretory Structures.
28155165	0	16	theme	Secretory	32:40	arg1	Structures					42:51	Plant Secretory Structures	26:51	Plant Secretory Structures	26:51	Histochemical Analysis of Plant Secretory Structures.
28155165	3	17	theme	secretory	551:559	arg1	structures					561:570	the secretory structures	547:570	the secretory structures	547:570	Here I describe some of the most used methods to detect carbohydrates, proteins, lipids, phenolic compounds, and alkaloids in the secretory structures.
28155165	2	18	theme	secretion	309:317	arg1	analysis					293:300	a correct analysis	283:300	a correct analysis of its secretion	283:317	As each gland may produce one or more types of substances, a correct analysis of its secretion should be done using various histochemical tests to detect metabolites of different chemical classes.
28155165	3	19	theme	used	454:457	arg1	methods					459:465	the most used methods	445:465	the most used methods	445:465	Here I describe some of the most used methods to detect carbohydrates, proteins, lipids, phenolic compounds, and alkaloids in the secretory structures.
28155165	1	20	theme	structures	123:132	arg1	study					98:102	the study	94:102	the study of plant secretory structures whose classification is based, at least partially, on the composition of their secretion	94:221	Histochemical analysis is essential for the study of plant secretory structures whose classification is based, at least partially, on the composition of their secretion.
28155165	3	21	from	compounds	519:527	arg1	structures					561:570	the secretory structures	547:570	the secretory structures	547:570	Here I describe some of the most used methods to detect carbohydrates, proteins, lipids, phenolic compounds, and alkaloids in the secretory structures.
28155165	1	22	theme	secretion	213:221	arg1	composition					192:202	the composition	188:202	the composition of their secretion	188:221	Histochemical analysis is essential for the study of plant secretory structures whose classification is based, at least partially, on the composition of their secretion.
28155165	1	23	theme	Histochemical	54:66	arg1	analysis					68:75	Histochemical analysis	54:75	Histochemical analysis	54:75	Histochemical analysis is essential for the study of plant secretory structures whose classification is based, at least partially, on the composition of their secretion.
28155165	3	24	from	proteins	492:499	arg1	structures					561:570	the secretory structures	547:570	the secretory structures	547:570	Here I describe some of the most used methods to detect carbohydrates, proteins, lipids, phenolic compounds, and alkaloids in the secretory structures.
28334971	8	0	theme	noncognate	1466:1475	arg1	synthase					1477:1484	their noncognate synthase	1460:1484	their noncognate synthase	1460:1484	In addition, we created chimeric block polymers composed of both HA and HEP segments; in these reactions GlcA-, but not N-acetylglucosamine-(GlcNAc), terminated GAG acceptors were recognized by their noncognate synthase for further extension, likely due to the common β-linkage connecting GlcA to GlcNAc in both of these GAGs.
28334971	9	1	theme	new	1631:1633	arg1	tools					1635:1639	new tools	1631:1639	new tools for studying biology	1631:1660	Overall, these GAG constructs provide new tools for studying biology and offer potential for future sugar-based therapeutics.
28334971	3	2	theme	GAG	514:516	arg1	synthases					518:526	current GAG synthases	506:526	current GAG synthases	506:526	Unfortunately, the in vitro enzymatic synthesis of defined GAGs is quite restricted by a limited understanding of current GAG synthases and modifying enzymes.
28334971	5	3	from	multocida	938:946	arg1	synthase					900:907	the HA synthase	893:907	the HA synthase from the microbe Pasteurella multocida	893:946	We show that a C6-sulfonated uridine diphospho (UDP)-glucose (Glc) derivative, sulfoquinovose, can be used as an uronic acid donor, but not as a hexosamine donor, to cap hyaluronan (HA) chains by the HA synthase from the microbe Pasteurella multocida.
28334971	8	4	theme	common	1527:1532	arg1	β-linkage					1534:1542	the common β-linkage	1523:1542	the common β-linkage connecting GlcA to GlcNAc in both of these GAGs	1523:1590	In addition, we created chimeric block polymers composed of both HA and HEP segments; in these reactions GlcA-, but not N-acetylglucosamine-(GlcNAc), terminated GAG acceptors were recognized by their noncognate synthase for further extension, likely due to the common β-linkage connecting GlcA to GlcNAc in both of these GAGs.
28334971	0	5	theme	analogs	77:83	arg1	creation					56:63	the creation	52:63	the creation of sulfated analogs, novel polymers and chimeric constructs	52:123	Expanding glycosaminoglycan chemical space: towards the creation of sulfated analogs, novel polymers and chimeric constructs.
28334971	5	6	theme	hyaluronan	867:876	arg1	chains					883:888	cap hyaluronan (HA) chains	863:888	cap hyaluronan (HA) chains	863:888	We show that a C6-sulfonated uridine diphospho (UDP)-glucose (Glc) derivative, sulfoquinovose, can be used as an uronic acid donor, but not as a hexosamine donor, to cap hyaluronan (HA) chains by the HA synthase from the microbe Pasteurella multocida.
28334971	3	7	theme	synthases	518:526	arg1	understanding					489:501	a limited understanding	479:501	a limited understanding of current GAG synthases and modifying enzymes	479:548	Unfortunately, the in vitro enzymatic synthesis of defined GAGs is quite restricted by a limited understanding of current GAG synthases and modifying enzymes.
28334971	8	8	dep	reactions	1361:1369	arg1	GlcA-					1371:1375	GlcA-	1371:1375	GlcA-	1371:1375	In addition, we created chimeric block polymers composed of both HA and HEP segments; in these reactions GlcA-, but not N-acetylglucosamine-(GlcNAc), terminated GAG acceptors were recognized by their noncognate synthase for further extension, likely due to the common β-linkage connecting GlcA to GlcNAc in both of these GAGs.
28334971	8	8	dep	reactions	1361:1369	arg1	GlcNAc					1407:1412	GlcNAc	1407:1412	GlcNAc	1407:1412	In addition, we created chimeric block polymers composed of both HA and HEP segments; in these reactions GlcA-, but not N-acetylglucosamine-(GlcNAc), terminated GAG acceptors were recognized by their noncognate synthase for further extension, likely due to the common β-linkage connecting GlcA to GlcNAc in both of these GAGs.
28334971	8	8	dep	reactions	1361:1369	arg1	N-acetylglucosamine-					1386:1405	N-acetylglucosamine-	1386:1405	N-acetylglucosamine-(GlcNAc)	1386:1413	In addition, we created chimeric block polymers composed of both HA and HEP segments; in these reactions GlcA-, but not N-acetylglucosamine-(GlcNAc), terminated GAG acceptors were recognized by their noncognate synthase for further extension, likely due to the common β-linkage connecting GlcA to GlcNAc in both of these GAGs.
28334971	0	9	theme	polymers	92:99	arg1	creation					56:63	the creation	52:63	the creation of sulfated analogs, novel polymers and chimeric constructs	52:123	Expanding glycosaminoglycan chemical space: towards the creation of sulfated analogs, novel polymers and chimeric constructs.
28334971	2	10	theme	structural	294:303	arg1	composition					336:346	disaccharide composition	323:346	disaccharide composition	323:346	GAG bioactivity is conferred by intrinsic structural features, such as disaccharide composition, glycosidic linkages and sulfation pattern.
28334971	2	10	theme	structural	294:303	arg1	linkages					360:367	glycosidic linkages	349:367	glycosidic linkages	349:367	GAG bioactivity is conferred by intrinsic structural features, such as disaccharide composition, glycosidic linkages and sulfation pattern.
28334971	2	10	theme	structural	294:303	arg1	features					305:312	intrinsic structural features	284:312	intrinsic structural features	284:312	GAG bioactivity is conferred by intrinsic structural features, such as disaccharide composition, glycosidic linkages and sulfation pattern.
28334971	2	10	theme	structural	294:303	arg1	pattern					383:389	sulfation pattern	373:389	sulfation pattern	373:389	GAG bioactivity is conferred by intrinsic structural features, such as disaccharide composition, glycosidic linkages and sulfation pattern.
28334971	8	11	theme	block	1299:1303	arg1	polymers					1305:1312	chimeric block polymers	1290:1312	chimeric block polymers composed of both HA and HEP segments	1290:1349	In addition, we created chimeric block polymers composed of both HA and HEP segments; in these reactions GlcA-, but not N-acetylglucosamine-(GlcNAc), terminated GAG acceptors were recognized by their noncognate synthase for further extension, likely due to the common β-linkage connecting GlcA to GlcNAc in both of these GAGs.
28334971	0	12	theme	novel	86:90	arg1	polymers					92:99	novel polymers	86:99	novel polymers	86:99	Expanding glycosaminoglycan chemical space: towards the creation of sulfated analogs, novel polymers and chimeric constructs.
28334971	8	13	theme	GAG	1427:1429	arg1	acceptors					1431:1439	terminated GAG acceptors	1416:1439	terminated GAG acceptors	1416:1439	In addition, we created chimeric block polymers composed of both HA and HEP segments; in these reactions GlcA-, but not N-acetylglucosamine-(GlcNAc), terminated GAG acceptors were recognized by their noncognate synthase for further extension, likely due to the common β-linkage connecting GlcA to GlcNAc in both of these GAGs.
28334971	5	14	theme	microbe	918:924	arg1	multocida					938:946	the microbe Pasteurella multocida	914:946	the microbe Pasteurella multocida	914:946	We show that a C6-sulfonated uridine diphospho (UDP)-glucose (Glc) derivative, sulfoquinovose, can be used as an uronic acid donor, but not as a hexosamine donor, to cap hyaluronan (HA) chains by the HA synthase from the microbe Pasteurella multocida.
28334971	2	15	theme	intrinsic	284:292	arg1	composition					336:346	disaccharide composition	323:346	disaccharide composition	323:346	GAG bioactivity is conferred by intrinsic structural features, such as disaccharide composition, glycosidic linkages and sulfation pattern.
28334971	2	15	theme	intrinsic	284:292	arg1	linkages					360:367	glycosidic linkages	349:367	glycosidic linkages	349:367	GAG bioactivity is conferred by intrinsic structural features, such as disaccharide composition, glycosidic linkages and sulfation pattern.
28334971	2	15	theme	intrinsic	284:292	arg1	features					305:312	intrinsic structural features	284:312	intrinsic structural features	284:312	GAG bioactivity is conferred by intrinsic structural features, such as disaccharide composition, glycosidic linkages and sulfation pattern.
28334971	2	15	theme	intrinsic	284:292	arg1	pattern					383:389	sulfation pattern	373:389	sulfation pattern	373:389	GAG bioactivity is conferred by intrinsic structural features, such as disaccharide composition, glycosidic linkages and sulfation pattern.
28334971	7	16	theme	novel	1194:1198	arg1	polymer					1209:1215	a novel HEP-like polymer	1192:1215	a novel HEP-like polymer we named hepbiuronic acid [-4-GlcAβ1-4-Glcα1-]n	1192:1263	Serendipitously, we found that PmHS2 co-polymerized Glc with glucuronic acid (GlcA), creating a novel HEP-like polymer we named hepbiuronic acid [-4-GlcAβ1-4-Glcα1-]n.
28334971	0	17	theme	chimeric	105:112	arg1	constructs					114:123	chimeric constructs	105:123	chimeric constructs	105:123	Expanding glycosaminoglycan chemical space: towards the creation of sulfated analogs, novel polymers and chimeric constructs.
28334971	1	18	contain	have	152:155	arg1	Glycosaminoglycans					126:143	Glycosaminoglycans	126:143	Glycosaminoglycans (GAGs)	126:150	Glycosaminoglycans (GAGs) have therapeutic potential in areas ranging from angiogenesis, inflammation, hemostasis and cancer.
28334971	1	18	contain	have	152:155	arg2	potential					169:177	therapeutic potential	157:177	therapeutic potential	157:177	Glycosaminoglycans (GAGs) have therapeutic potential in areas ranging from angiogenesis, inflammation, hemostasis and cancer.
28334971	1	18	contain	have	152:155	arg1	GAGs					146:149	GAGs	146:149	GAGs	146:149	Glycosaminoglycans (GAGs) have therapeutic potential in areas ranging from angiogenesis, inflammation, hemostasis and cancer.
28334971	3	19	dep	in	411:412	arg1	vitro					414:418	vitro	414:418	vitro	414:418	Unfortunately, the in vitro enzymatic synthesis of defined GAGs is quite restricted by a limited understanding of current GAG synthases and modifying enzymes.
28334971	3	20	theme	enzymes	542:548	arg1	understanding					489:501	a limited understanding	479:501	a limited understanding of current GAG synthases and modifying enzymes	479:548	Unfortunately, the in vitro enzymatic synthesis of defined GAGs is quite restricted by a limited understanding of current GAG synthases and modifying enzymes.
28334971	9	21	theme	sugar-based	1693:1703	arg1	therapeutics					1705:1716	future sugar-based therapeutics	1686:1716	future sugar-based therapeutics	1686:1716	Overall, these GAG constructs provide new tools for studying biology and offer potential for future sugar-based therapeutics.
28334971	3	22	theme	GAGs	451:454	arg1	restricted					465:474	restricted	465:474	restricted	465:474	Unfortunately, the in vitro enzymatic synthesis of defined GAGs is quite restricted by a limited understanding of current GAG synthases and modifying enzymes.
28334971	3	22	theme	GAGs	451:454	arg1	synthesis					430:438	the in vitro enzymatic synthesis	407:438	the in vitro enzymatic synthesis of defined GAGs	407:454	Unfortunately, the in vitro enzymatic synthesis of defined GAGs is quite restricted by a limited understanding of current GAG synthases and modifying enzymes.
28334971	7	23	dep	polymer	1209:1215	arg1	we					1217:1218	we	1217:1218	we	1217:1218	Serendipitously, we found that PmHS2 co-polymerized Glc with glucuronic acid (GlcA), creating a novel HEP-like polymer we named hepbiuronic acid [-4-GlcAβ1-4-Glcα1-]n.
28334971	4	24	theme	chimeric	678:685	arg1	polymers					687:694	chimeric polymers	678:694	chimeric polymers	678:694	Our work provides insights into GAG-active enzymes through the creation of sulfated oligosaccharides, a new polysaccharide and chimeric polymers.
28334971	4	24	theme	chimeric	678:685	arg1	oligosaccharides					635:650	sulfated oligosaccharides	626:650	sulfated oligosaccharides	626:650	Our work provides insights into GAG-active enzymes through the creation of sulfated oligosaccharides, a new polysaccharide and chimeric polymers.
28334971	0	25	theme	glycosaminoglycan	10:26	arg1	space					37:41	glycosaminoglycan chemical space:	10:42	glycosaminoglycan chemical space:	10:42	Expanding glycosaminoglycan chemical space: towards the creation of sulfated analogs, novel polymers and chimeric constructs.
28334971	7	26	theme	glucuronic	1159:1168	arg1	GlcA					1176:1179	GlcA	1176:1179	GlcA	1176:1179	Serendipitously, we found that PmHS2 co-polymerized Glc with glucuronic acid (GlcA), creating a novel HEP-like polymer we named hepbiuronic acid [-4-GlcAβ1-4-Glcα1-]n.
28334971	7	26	theme	glucuronic	1159:1168	arg1	acid					1170:1173	glucuronic acid	1159:1173	glucuronic acid (GlcA)	1159:1180	Serendipitously, we found that PmHS2 co-polymerized Glc with glucuronic acid (GlcA), creating a novel HEP-like polymer we named hepbiuronic acid [-4-GlcAβ1-4-Glcα1-]n.
28334971	8	27	theme	chimeric	1290:1297	arg1	polymers					1305:1312	chimeric block polymers	1290:1312	chimeric block polymers composed of both HA and HEP segments	1290:1349	In addition, we created chimeric block polymers composed of both HA and HEP segments; in these reactions GlcA-, but not N-acetylglucosamine-(GlcNAc), terminated GAG acceptors were recognized by their noncognate synthase for further extension, likely due to the common β-linkage connecting GlcA to GlcNAc in both of these GAGs.
28334971	8	28	theme	terminated	1416:1425	arg1	acceptors					1431:1439	terminated GAG acceptors	1416:1439	terminated GAG acceptors	1416:1439	In addition, we created chimeric block polymers composed of both HA and HEP segments; in these reactions GlcA-, but not N-acetylglucosamine-(GlcNAc), terminated GAG acceptors were recognized by their noncognate synthase for further extension, likely due to the common β-linkage connecting GlcA to GlcNAc in both of these GAGs.
28334971	7	29	theme	[-4-GlcAβ1-4-Glcα1-	1243:1261	arg1	n					1263:1263	hepbiuronic acid [-4-GlcAβ1-4-Glcα1-]n	1226:1263	hepbiuronic acid [-4-GlcAβ1-4-Glcα1-]n	1226:1263	Serendipitously, we found that PmHS2 co-polymerized Glc with glucuronic acid (GlcA), creating a novel HEP-like polymer we named hepbiuronic acid [-4-GlcAβ1-4-Glcα1-]n.
28334971	5	30	theme	uronic	810:815	arg1	derivative					764:773	a C6-sulfonated uridine diphospho (UDP)-glucose (Glc) derivative	710:773	a C6-sulfonated uridine diphospho (UDP)-glucose (Glc) derivative	710:773	We show that a C6-sulfonated uridine diphospho (UDP)-glucose (Glc) derivative, sulfoquinovose, can be used as an uronic acid donor, but not as a hexosamine donor, to cap hyaluronan (HA) chains by the HA synthase from the microbe Pasteurella multocida.
28334971	5	30	theme	uronic	810:815	arg1	donor					822:826	an uronic acid donor	807:826	an uronic acid donor	807:826	We show that a C6-sulfonated uridine diphospho (UDP)-glucose (Glc) derivative, sulfoquinovose, can be used as an uronic acid donor, but not as a hexosamine donor, to cap hyaluronan (HA) chains by the HA synthase from the microbe Pasteurella multocida.
28334971	5	31	theme	HA	897:898	arg1	synthase					900:907	the HA synthase	893:907	the HA synthase from the microbe Pasteurella multocida	893:946	We show that a C6-sulfonated uridine diphospho (UDP)-glucose (Glc) derivative, sulfoquinovose, can be used as an uronic acid donor, but not as a hexosamine donor, to cap hyaluronan (HA) chains by the HA synthase from the microbe Pasteurella multocida.
28334971	3	32	theme	defined	443:449	arg1	GAGs					451:454	defined GAGs	443:454	defined GAGs	443:454	Unfortunately, the in vitro enzymatic synthesis of defined GAGs is quite restricted by a limited understanding of current GAG synthases and modifying enzymes.
28334971	5	33	theme	acid	817:820	arg1	derivative					764:773	a C6-sulfonated uridine diphospho (UDP)-glucose (Glc) derivative	710:773	a C6-sulfonated uridine diphospho (UDP)-glucose (Glc) derivative	710:773	We show that a C6-sulfonated uridine diphospho (UDP)-glucose (Glc) derivative, sulfoquinovose, can be used as an uronic acid donor, but not as a hexosamine donor, to cap hyaluronan (HA) chains by the HA synthase from the microbe Pasteurella multocida.
28334971	5	33	theme	acid	817:820	arg1	donor					822:826	an uronic acid donor	807:826	an uronic acid donor	807:826	We show that a C6-sulfonated uridine diphospho (UDP)-glucose (Glc) derivative, sulfoquinovose, can be used as an uronic acid donor, but not as a hexosamine donor, to cap hyaluronan (HA) chains by the HA synthase from the microbe Pasteurella multocida.
28334971	5	34	theme	diphospho	734:742	arg1	donor					853:857	a hexosamine donor	840:857	a hexosamine donor	840:857	We show that a C6-sulfonated uridine diphospho (UDP)-glucose (Glc) derivative, sulfoquinovose, can be used as an uronic acid donor, but not as a hexosamine donor, to cap hyaluronan (HA) chains by the HA synthase from the microbe Pasteurella multocida.
28334971	5	34	theme	diphospho	734:742	arg1	sulfoquinovose					776:789	sulfoquinovose	776:789	sulfoquinovose	776:789	We show that a C6-sulfonated uridine diphospho (UDP)-glucose (Glc) derivative, sulfoquinovose, can be used as an uronic acid donor, but not as a hexosamine donor, to cap hyaluronan (HA) chains by the HA synthase from the microbe Pasteurella multocida.
28334971	5	34	theme	diphospho	734:742	arg1	derivative					764:773	a C6-sulfonated uridine diphospho (UDP)-glucose (Glc) derivative	710:773	a C6-sulfonated uridine diphospho (UDP)-glucose (Glc) derivative	710:773	We show that a C6-sulfonated uridine diphospho (UDP)-glucose (Glc) derivative, sulfoquinovose, can be used as an uronic acid donor, but not as a hexosamine donor, to cap hyaluronan (HA) chains by the HA synthase from the microbe Pasteurella multocida.
28334971	5	34	theme	diphospho	734:742	arg1	donor					822:826	an uronic acid donor	807:826	an uronic acid donor	807:826	We show that a C6-sulfonated uridine diphospho (UDP)-glucose (Glc) derivative, sulfoquinovose, can be used as an uronic acid donor, but not as a hexosamine donor, to cap hyaluronan (HA) chains by the HA synthase from the microbe Pasteurella multocida.
28334971	6	35	theme	similar	1078:1084	arg1	conditions					1086:1095	similar conditions	1078:1095	similar conditions	1078:1095	However, the two heparosan (HEP) synthases from the same species, PmHS1 and PmHS2, could not employ the UDP-sulfoquinovose under similar conditions.
28334971	6	36	from	species	1006:1012	arg1	synthases					982:990	the two heparosan (HEP) synthases	958:990	the two heparosan (HEP) synthases from the same species, PmHS1 and PmHS2,	958:1030	However, the two heparosan (HEP) synthases from the same species, PmHS1 and PmHS2, could not employ the UDP-sulfoquinovose under similar conditions.
28334971	4	37	theme	new	655:657	arg1	polysaccharide					659:672	a new polysaccharide	653:672	a new polysaccharide	653:672	Our work provides insights into GAG-active enzymes through the creation of sulfated oligosaccharides, a new polysaccharide and chimeric polymers.
28334971	4	37	theme	new	655:657	arg1	oligosaccharides					635:650	sulfated oligosaccharides	626:650	sulfated oligosaccharides	626:650	Our work provides insights into GAG-active enzymes through the creation of sulfated oligosaccharides, a new polysaccharide and chimeric polymers.
28334971	0	38	theme	chemical	28:35	arg1	space					37:41	glycosaminoglycan chemical space:	10:42	glycosaminoglycan chemical space:	10:42	Expanding glycosaminoglycan chemical space: towards the creation of sulfated analogs, novel polymers and chimeric constructs.
28334971	4	39	theme	GAG-active	583:592	arg1	enzymes					594:600	GAG-active enzymes	583:600	GAG-active enzymes	583:600	Our work provides insights into GAG-active enzymes through the creation of sulfated oligosaccharides, a new polysaccharide and chimeric polymers.
28334971	0	40	theme	constructs	114:123	arg1	creation					56:63	the creation	52:63	the creation of sulfated analogs, novel polymers and chimeric constructs	52:123	Expanding glycosaminoglycan chemical space: towards the creation of sulfated analogs, novel polymers and chimeric constructs.
28334971	1	41	theme	therapeutic	157:167	arg1	potential					169:177	therapeutic potential	157:177	therapeutic potential	157:177	Glycosaminoglycans (GAGs) have therapeutic potential in areas ranging from angiogenesis, inflammation, hemostasis and cancer.
28334971	7	42	theme	acid	1238:1241	arg1	n					1263:1263	hepbiuronic acid [-4-GlcAβ1-4-Glcα1-]n	1226:1263	hepbiuronic acid [-4-GlcAβ1-4-Glcα1-]n	1226:1263	Serendipitously, we found that PmHS2 co-polymerized Glc with glucuronic acid (GlcA), creating a novel HEP-like polymer we named hepbiuronic acid [-4-GlcAβ1-4-Glcα1-]n.
28334971	3	43	theme	enzymatic	420:428	arg1	restricted					465:474	restricted	465:474	restricted	465:474	Unfortunately, the in vitro enzymatic synthesis of defined GAGs is quite restricted by a limited understanding of current GAG synthases and modifying enzymes.
28334971	3	43	theme	enzymatic	420:428	arg1	synthesis					430:438	the in vitro enzymatic synthesis	407:438	the in vitro enzymatic synthesis of defined GAGs	407:454	Unfortunately, the in vitro enzymatic synthesis of defined GAGs is quite restricted by a limited understanding of current GAG synthases and modifying enzymes.
28334971	5	44	theme	UDP	745:747	arg1	donor					853:857	a hexosamine donor	840:857	a hexosamine donor	840:857	We show that a C6-sulfonated uridine diphospho (UDP)-glucose (Glc) derivative, sulfoquinovose, can be used as an uronic acid donor, but not as a hexosamine donor, to cap hyaluronan (HA) chains by the HA synthase from the microbe Pasteurella multocida.
28334971	5	44	theme	UDP	745:747	arg1	sulfoquinovose					776:789	sulfoquinovose	776:789	sulfoquinovose	776:789	We show that a C6-sulfonated uridine diphospho (UDP)-glucose (Glc) derivative, sulfoquinovose, can be used as an uronic acid donor, but not as a hexosamine donor, to cap hyaluronan (HA) chains by the HA synthase from the microbe Pasteurella multocida.
28334971	5	44	theme	UDP	745:747	arg1	derivative					764:773	a C6-sulfonated uridine diphospho (UDP)-glucose (Glc) derivative	710:773	a C6-sulfonated uridine diphospho (UDP)-glucose (Glc) derivative	710:773	We show that a C6-sulfonated uridine diphospho (UDP)-glucose (Glc) derivative, sulfoquinovose, can be used as an uronic acid donor, but not as a hexosamine donor, to cap hyaluronan (HA) chains by the HA synthase from the microbe Pasteurella multocida.
28334971	5	44	theme	UDP	745:747	arg1	donor					822:826	an uronic acid donor	807:826	an uronic acid donor	807:826	We show that a C6-sulfonated uridine diphospho (UDP)-glucose (Glc) derivative, sulfoquinovose, can be used as an uronic acid donor, but not as a hexosamine donor, to cap hyaluronan (HA) chains by the HA synthase from the microbe Pasteurella multocida.
28334971	6	45	theme	same	1001:1004	arg1	species					1006:1012	the same species	997:1012	the same species	997:1012	However, the two heparosan (HEP) synthases from the same species, PmHS1 and PmHS2, could not employ the UDP-sulfoquinovose under similar conditions.
28334971	6	45	theme	same	1001:1004	arg1	PmHS2					1025:1029	PmHS2	1025:1029	PmHS2	1025:1029	However, the two heparosan (HEP) synthases from the same species, PmHS1 and PmHS2, could not employ the UDP-sulfoquinovose under similar conditions.
28334971	6	45	theme	same	1001:1004	arg1	PmHS1					1015:1019	PmHS1	1015:1019	PmHS1	1015:1019	However, the two heparosan (HEP) synthases from the same species, PmHS1 and PmHS2, could not employ the UDP-sulfoquinovose under similar conditions.
28334971	6	46	theme	HEP	977:979	arg1	synthases					982:990	the two heparosan (HEP) synthases	958:990	the two heparosan (HEP) synthases from the same species, PmHS1 and PmHS2,	958:1030	However, the two heparosan (HEP) synthases from the same species, PmHS1 and PmHS2, could not employ the UDP-sulfoquinovose under similar conditions.
28334971	5	47	theme	Glc	759:761	arg1	donor					853:857	a hexosamine donor	840:857	a hexosamine donor	840:857	We show that a C6-sulfonated uridine diphospho (UDP)-glucose (Glc) derivative, sulfoquinovose, can be used as an uronic acid donor, but not as a hexosamine donor, to cap hyaluronan (HA) chains by the HA synthase from the microbe Pasteurella multocida.
28334971	5	47	theme	Glc	759:761	arg1	sulfoquinovose					776:789	sulfoquinovose	776:789	sulfoquinovose	776:789	We show that a C6-sulfonated uridine diphospho (UDP)-glucose (Glc) derivative, sulfoquinovose, can be used as an uronic acid donor, but not as a hexosamine donor, to cap hyaluronan (HA) chains by the HA synthase from the microbe Pasteurella multocida.
28334971	5	47	theme	Glc	759:761	arg1	derivative					764:773	a C6-sulfonated uridine diphospho (UDP)-glucose (Glc) derivative	710:773	a C6-sulfonated uridine diphospho (UDP)-glucose (Glc) derivative	710:773	We show that a C6-sulfonated uridine diphospho (UDP)-glucose (Glc) derivative, sulfoquinovose, can be used as an uronic acid donor, but not as a hexosamine donor, to cap hyaluronan (HA) chains by the HA synthase from the microbe Pasteurella multocida.
28334971	5	47	theme	Glc	759:761	arg1	donor					822:826	an uronic acid donor	807:826	an uronic acid donor	807:826	We show that a C6-sulfonated uridine diphospho (UDP)-glucose (Glc) derivative, sulfoquinovose, can be used as an uronic acid donor, but not as a hexosamine donor, to cap hyaluronan (HA) chains by the HA synthase from the microbe Pasteurella multocida.
28334971	3	48	theme	in	411:412	arg1	restricted					465:474	restricted	465:474	restricted	465:474	Unfortunately, the in vitro enzymatic synthesis of defined GAGs is quite restricted by a limited understanding of current GAG synthases and modifying enzymes.
28334971	3	48	theme	in	411:412	arg1	synthesis					430:438	the in vitro enzymatic synthesis	407:438	the in vitro enzymatic synthesis of defined GAGs	407:454	Unfortunately, the in vitro enzymatic synthesis of defined GAGs is quite restricted by a limited understanding of current GAG synthases and modifying enzymes.
28334971	5	49	theme	-glucose	749:756	arg1	donor					853:857	a hexosamine donor	840:857	a hexosamine donor	840:857	We show that a C6-sulfonated uridine diphospho (UDP)-glucose (Glc) derivative, sulfoquinovose, can be used as an uronic acid donor, but not as a hexosamine donor, to cap hyaluronan (HA) chains by the HA synthase from the microbe Pasteurella multocida.
28334971	5	49	theme	-glucose	749:756	arg1	sulfoquinovose					776:789	sulfoquinovose	776:789	sulfoquinovose	776:789	We show that a C6-sulfonated uridine diphospho (UDP)-glucose (Glc) derivative, sulfoquinovose, can be used as an uronic acid donor, but not as a hexosamine donor, to cap hyaluronan (HA) chains by the HA synthase from the microbe Pasteurella multocida.
28334971	5	49	theme	-glucose	749:756	arg1	derivative					764:773	a C6-sulfonated uridine diphospho (UDP)-glucose (Glc) derivative	710:773	a C6-sulfonated uridine diphospho (UDP)-glucose (Glc) derivative	710:773	We show that a C6-sulfonated uridine diphospho (UDP)-glucose (Glc) derivative, sulfoquinovose, can be used as an uronic acid donor, but not as a hexosamine donor, to cap hyaluronan (HA) chains by the HA synthase from the microbe Pasteurella multocida.
28334971	5	49	theme	-glucose	749:756	arg1	donor					822:826	an uronic acid donor	807:826	an uronic acid donor	807:826	We show that a C6-sulfonated uridine diphospho (UDP)-glucose (Glc) derivative, sulfoquinovose, can be used as an uronic acid donor, but not as a hexosamine donor, to cap hyaluronan (HA) chains by the HA synthase from the microbe Pasteurella multocida.
28334971	9	50	theme	GAG	1608:1610	arg1	constructs					1612:1621	these GAG constructs	1602:1621	these GAG constructs	1602:1621	Overall, these GAG constructs provide new tools for studying biology and offer potential for future sugar-based therapeutics.
28334971	2	51	theme	glycosidic	349:358	arg1	linkages					360:367	glycosidic linkages	349:367	glycosidic linkages	349:367	GAG bioactivity is conferred by intrinsic structural features, such as disaccharide composition, glycosidic linkages and sulfation pattern.
28334971	2	52	theme	sulfation	373:381	arg1	pattern					383:389	sulfation pattern	373:389	sulfation pattern	373:389	GAG bioactivity is conferred by intrinsic structural features, such as disaccharide composition, glycosidic linkages and sulfation pattern.
28334971	7	53	with	Glc	1150:1152	arg1	GlcA					1176:1179	GlcA	1176:1179	GlcA	1176:1179	Serendipitously, we found that PmHS2 co-polymerized Glc with glucuronic acid (GlcA), creating a novel HEP-like polymer we named hepbiuronic acid [-4-GlcAβ1-4-Glcα1-]n.
28334971	7	53	with	Glc	1150:1152	arg1	acid					1170:1173	glucuronic acid	1159:1173	glucuronic acid (GlcA)	1159:1180	Serendipitously, we found that PmHS2 co-polymerized Glc with glucuronic acid (GlcA), creating a novel HEP-like polymer we named hepbiuronic acid [-4-GlcAβ1-4-Glcα1-]n.
28334971	7	54	theme	HEP-like	1200:1207	arg1	polymer					1209:1215	a novel HEP-like polymer	1192:1215	a novel HEP-like polymer we named hepbiuronic acid [-4-GlcAβ1-4-Glcα1-]n	1192:1263	Serendipitously, we found that PmHS2 co-polymerized Glc with glucuronic acid (GlcA), creating a novel HEP-like polymer we named hepbiuronic acid [-4-GlcAβ1-4-Glcα1-]n.
28334971	3	55	theme	current	506:512	arg1	synthases					518:526	current GAG synthases	506:526	current GAG synthases	506:526	Unfortunately, the in vitro enzymatic synthesis of defined GAGs is quite restricted by a limited understanding of current GAG synthases and modifying enzymes.
28334971	8	56	from	GlcNAc	1563:1568	arg1	both					1573:1576	both	1573:1576	both	1573:1576	In addition, we created chimeric block polymers composed of both HA and HEP segments; in these reactions GlcA-, but not N-acetylglucosamine-(GlcNAc), terminated GAG acceptors were recognized by their noncognate synthase for further extension, likely due to the common β-linkage connecting GlcA to GlcNAc in both of these GAGs.
28334971	8	56	from	GlcNAc	1563:1568	arg1	GAGs					1587:1590	these GAGs	1581:1590	these GAGs	1581:1590	In addition, we created chimeric block polymers composed of both HA and HEP segments; in these reactions GlcA-, but not N-acetylglucosamine-(GlcNAc), terminated GAG acceptors were recognized by their noncognate synthase for further extension, likely due to the common β-linkage connecting GlcA to GlcNAc in both of these GAGs.
28334971	8	57	theme	further	1490:1496	arg1	extension					1498:1506	further extension	1490:1506	further extension	1490:1506	In addition, we created chimeric block polymers composed of both HA and HEP segments; in these reactions GlcA-, but not N-acetylglucosamine-(GlcNAc), terminated GAG acceptors were recognized by their noncognate synthase for further extension, likely due to the common β-linkage connecting GlcA to GlcNAc in both of these GAGs.
28334971	8	58	theme	HEP	1338:1340	arg1	segments					1342:1349	HEP segments	1338:1349	HEP segments	1338:1349	In addition, we created chimeric block polymers composed of both HA and HEP segments; in these reactions GlcA-, but not N-acetylglucosamine-(GlcNAc), terminated GAG acceptors were recognized by their noncognate synthase for further extension, likely due to the common β-linkage connecting GlcA to GlcNAc in both of these GAGs.
28334971	3	59	theme	limited	481:487	arg1	understanding					489:501	a limited understanding	479:501	a limited understanding of current GAG synthases and modifying enzymes	479:548	Unfortunately, the in vitro enzymatic synthesis of defined GAGs is quite restricted by a limited understanding of current GAG synthases and modifying enzymes.
28334971	4	60	theme	sulfated	626:633	arg1	polysaccharide					659:672	a new polysaccharide	653:672	a new polysaccharide	653:672	Our work provides insights into GAG-active enzymes through the creation of sulfated oligosaccharides, a new polysaccharide and chimeric polymers.
28334971	4	60	theme	sulfated	626:633	arg1	polymers					687:694	chimeric polymers	678:694	chimeric polymers	678:694	Our work provides insights into GAG-active enzymes through the creation of sulfated oligosaccharides, a new polysaccharide and chimeric polymers.
28334971	4	60	theme	sulfated	626:633	arg1	oligosaccharides					635:650	sulfated oligosaccharides	626:650	sulfated oligosaccharides	626:650	Our work provides insights into GAG-active enzymes through the creation of sulfated oligosaccharides, a new polysaccharide and chimeric polymers.
28334971	5	61	theme	C6-sulfonated uridine	712:732	arg1	donor					853:857	a hexosamine donor	840:857	a hexosamine donor	840:857	We show that a C6-sulfonated uridine diphospho (UDP)-glucose (Glc) derivative, sulfoquinovose, can be used as an uronic acid donor, but not as a hexosamine donor, to cap hyaluronan (HA) chains by the HA synthase from the microbe Pasteurella multocida.
28334971	5	61	theme	C6-sulfonated uridine	712:732	arg1	sulfoquinovose					776:789	sulfoquinovose	776:789	sulfoquinovose	776:789	We show that a C6-sulfonated uridine diphospho (UDP)-glucose (Glc) derivative, sulfoquinovose, can be used as an uronic acid donor, but not as a hexosamine donor, to cap hyaluronan (HA) chains by the HA synthase from the microbe Pasteurella multocida.
28334971	5	61	theme	C6-sulfonated uridine	712:732	arg1	derivative					764:773	a C6-sulfonated uridine diphospho (UDP)-glucose (Glc) derivative	710:773	a C6-sulfonated uridine diphospho (UDP)-glucose (Glc) derivative	710:773	We show that a C6-sulfonated uridine diphospho (UDP)-glucose (Glc) derivative, sulfoquinovose, can be used as an uronic acid donor, but not as a hexosamine donor, to cap hyaluronan (HA) chains by the HA synthase from the microbe Pasteurella multocida.
28334971	5	61	theme	C6-sulfonated uridine	712:732	arg1	donor					822:826	an uronic acid donor	807:826	an uronic acid donor	807:826	We show that a C6-sulfonated uridine diphospho (UDP)-glucose (Glc) derivative, sulfoquinovose, can be used as an uronic acid donor, but not as a hexosamine donor, to cap hyaluronan (HA) chains by the HA synthase from the microbe Pasteurella multocida.
28334971	2	62	theme	disaccharide	323:334	arg1	composition					336:346	disaccharide composition	323:346	disaccharide composition	323:346	GAG bioactivity is conferred by intrinsic structural features, such as disaccharide composition, glycosidic linkages and sulfation pattern.
28334971	9	63	theme	future	1686:1691	arg1	therapeutics					1705:1716	future sugar-based therapeutics	1686:1716	future sugar-based therapeutics	1686:1716	Overall, these GAG constructs provide new tools for studying biology and offer potential for future sugar-based therapeutics.
28334971	0	64	theme	sulfated	68:75	arg1	analogs					77:83	sulfated analogs	68:83	sulfated analogs	68:83	Expanding glycosaminoglycan chemical space: towards the creation of sulfated analogs, novel polymers and chimeric constructs.
28334971	5	65	theme	Pasteurella	926:936	arg1	multocida					938:946	the microbe Pasteurella multocida	914:946	the microbe Pasteurella multocida	914:946	We show that a C6-sulfonated uridine diphospho (UDP)-glucose (Glc) derivative, sulfoquinovose, can be used as an uronic acid donor, but not as a hexosamine donor, to cap hyaluronan (HA) chains by the HA synthase from the microbe Pasteurella multocida.
28334971	2	66	theme	GAG	252:254	arg1	bioactivity					256:266	GAG bioactivity	252:266	GAG bioactivity	252:266	GAG bioactivity is conferred by intrinsic structural features, such as disaccharide composition, glycosidic linkages and sulfation pattern.
28334971	7	67	theme	hepbiuronic	1226:1236	arg1	acid					1238:1241	hepbiuronic acid	1226:1241	hepbiuronic acid [-4-GlcAβ1-4-Glcα1-]n	1226:1263	Serendipitously, we found that PmHS2 co-polymerized Glc with glucuronic acid (GlcA), creating a novel HEP-like polymer we named hepbiuronic acid [-4-GlcAβ1-4-Glcα1-]n.
28334971	5	68	theme	cap	863:865	arg1	hyaluronan					867:876	cap hyaluronan	863:876	cap hyaluronan (HA) chains	863:888	We show that a C6-sulfonated uridine diphospho (UDP)-glucose (Glc) derivative, sulfoquinovose, can be used as an uronic acid donor, but not as a hexosamine donor, to cap hyaluronan (HA) chains by the HA synthase from the microbe Pasteurella multocida.
28334971	5	68	theme	cap	863:865	arg1	HA					879:880	HA	879:880	HA	879:880	We show that a C6-sulfonated uridine diphospho (UDP)-glucose (Glc) derivative, sulfoquinovose, can be used as an uronic acid donor, but not as a hexosamine donor, to cap hyaluronan (HA) chains by the HA synthase from the microbe Pasteurella multocida.
28334971	5	69	theme	hexosamine	842:851	arg1	donor					853:857	a hexosamine donor	840:857	a hexosamine donor	840:857	We show that a C6-sulfonated uridine diphospho (UDP)-glucose (Glc) derivative, sulfoquinovose, can be used as an uronic acid donor, but not as a hexosamine donor, to cap hyaluronan (HA) chains by the HA synthase from the microbe Pasteurella multocida.
28334971	5	69	theme	hexosamine	842:851	arg1	derivative					764:773	a C6-sulfonated uridine diphospho (UDP)-glucose (Glc) derivative	710:773	a C6-sulfonated uridine diphospho (UDP)-glucose (Glc) derivative	710:773	We show that a C6-sulfonated uridine diphospho (UDP)-glucose (Glc) derivative, sulfoquinovose, can be used as an uronic acid donor, but not as a hexosamine donor, to cap hyaluronan (HA) chains by the HA synthase from the microbe Pasteurella multocida.
28334971	6	70	theme	heparosan	966:974	arg1	synthases					982:990	the two heparosan (HEP) synthases	958:990	the two heparosan (HEP) synthases from the same species, PmHS1 and PmHS2,	958:1030	However, the two heparosan (HEP) synthases from the same species, PmHS1 and PmHS2, could not employ the UDP-sulfoquinovose under similar conditions.
28334971	5	71	used	used	799:802	arg2	donor					822:826	an uronic acid donor	807:826	an uronic acid donor	807:826	We show that a C6-sulfonated uridine diphospho (UDP)-glucose (Glc) derivative, sulfoquinovose, can be used as an uronic acid donor, but not as a hexosamine donor, to cap hyaluronan (HA) chains by the HA synthase from the microbe Pasteurella multocida.
28334971	5	71	used	used	799:802	arg2	sulfoquinovose					776:789	sulfoquinovose	776:789	sulfoquinovose	776:789	We show that a C6-sulfonated uridine diphospho (UDP)-glucose (Glc) derivative, sulfoquinovose, can be used as an uronic acid donor, but not as a hexosamine donor, to cap hyaluronan (HA) chains by the HA synthase from the microbe Pasteurella multocida.
28334971	5	71	used	used	799:802	arg2	donor					853:857	a hexosamine donor	840:857	a hexosamine donor	840:857	We show that a C6-sulfonated uridine diphospho (UDP)-glucose (Glc) derivative, sulfoquinovose, can be used as an uronic acid donor, but not as a hexosamine donor, to cap hyaluronan (HA) chains by the HA synthase from the microbe Pasteurella multocida.
28334971	5	71	used	used	799:802	arg2	derivative					764:773	a C6-sulfonated uridine diphospho (UDP)-glucose (Glc) derivative	710:773	a C6-sulfonated uridine diphospho (UDP)-glucose (Glc) derivative	710:773	We show that a C6-sulfonated uridine diphospho (UDP)-glucose (Glc) derivative, sulfoquinovose, can be used as an uronic acid donor, but not as a hexosamine donor, to cap hyaluronan (HA) chains by the HA synthase from the microbe Pasteurella multocida.
28334971	8	72	theme	due	1516:1518	arg1	extension					1498:1506	further extension	1490:1506	further extension	1490:1506	In addition, we created chimeric block polymers composed of both HA and HEP segments; in these reactions GlcA-, but not N-acetylglucosamine-(GlcNAc), terminated GAG acceptors were recognized by their noncognate synthase for further extension, likely due to the common β-linkage connecting GlcA to GlcNAc in both of these GAGs.
28334971	3	73	theme	modifying	532:540	arg1	enzymes					542:548	modifying enzymes	532:548	modifying enzymes	532:548	Unfortunately, the in vitro enzymatic synthesis of defined GAGs is quite restricted by a limited understanding of current GAG synthases and modifying enzymes.
28334971	4	74	theme	oligosaccharides	635:650	arg1	creation					614:621	the creation	610:621	the creation of sulfated oligosaccharides, a new polysaccharide and chimeric polymers	610:694	Our work provides insights into GAG-active enzymes through the creation of sulfated oligosaccharides, a new polysaccharide and chimeric polymers.
27449907	2	0	theme	proteins	578:585	arg1	context					546:552	the context	542:552	the context of full length envelope proteins	542:585	Because both of these epitopes are poorly immunogenic in the context of full length envelope proteins, immunization with properly folded and glycosylated fragments (scaffolds) represents a potential way to enhance the immune response to these specific epitopes.
27449907	7	1	from	clades	1720:1725	arg1	scaffolds					1689:1697	engineered glycopeptide scaffolds	1665:1697	engineered glycopeptide scaffolds from three different clades of HIV-1 that bind PG9 with high affinity when expressed in a wildtype cell line suitable for biopharmaceutical production	1665:1848	In this report, we describe engineered glycopeptide scaffolds from three different clades of HIV-1 that bind PG9 with high affinity when expressed in a wildtype cell line suitable for biopharmaceutical production.
27449907	2	2	theme	length	562:567	arg1	proteins					578:585	full length envelope proteins	557:585	full length envelope proteins	557:585	Because both of these epitopes are poorly immunogenic in the context of full length envelope proteins, immunization with properly folded and glycosylated fragments (scaffolds) represents a potential way to enhance the immune response to these specific epitopes.
27449907	9	3	dep	responses	2243:2251	arg1	2					2219:2219	2	2219:2219	2	2219:2219	The scaffolds produced address three major problems in HIV vaccine development: (1) improving antibody responses to poorly immunogenic epitopes in the V1/V2 domain; (2) eliminating antibody responses to highly immunogenic (decoy) epitopes outside the V1/V2 domain; and (3) enabling the production of V1/V2 scaffolds in a cell line suitable for biopharmaceutical production.
27449907	4	4	theme	conformational	1003:1016	arg1	stability					1018:1026	conformational stability	1003:1026	conformational stability	1003:1026	In this paper, we used a protein engineering approach to improve the conformational stability and antibody binding activity of V1/V2 domain scaffolds from multiple diverse isolates, including several that were initially unable to bind the prototypic PG9 bN-mAb.
27449907	6	5	theme	mutant	1496:1501	arg1	lines					1508:1512	mutant cell lines	1496:1512	mutant cell lines (e.g. GnTI(-) 293 HEK)	1496:1535	Although scaffolds incorporating the glycans required for PG9 binding (e.g., mannose-5) can be produced using glycosylation inhibitors (e.g., swainsonine), or mutant cell lines (e.g. GnTI(-) 293 HEK), these are not practical for biopharmaceutical production of proteins intended for clinical trials.
27449907	6	5	theme	mutant	1496:1501	arg1	HEK					1532:1534	293 HEK	1528:1534	293 HEK	1528:1534	Although scaffolds incorporating the glycans required for PG9 binding (e.g., mannose-5) can be produced using glycosylation inhibitors (e.g., swainsonine), or mutant cell lines (e.g. GnTI(-) 293 HEK), these are not practical for biopharmaceutical production of proteins intended for clinical trials.
27449907	7	6	theme	different	1710:1718	arg1	clades					1720:1725	three different clades	1704:1725	three different clades of HIV-1	1704:1734	In this report, we describe engineered glycopeptide scaffolds from three different clades of HIV-1 that bind PG9 with high affinity when expressed in a wildtype cell line suitable for biopharmaceutical production.
27449907	2	7	theme	specific	728:735	arg1	epitopes					737:744	these specific epitopes	722:744	these specific epitopes	722:744	Because both of these epitopes are poorly immunogenic in the context of full length envelope proteins, immunization with properly folded and glycosylated fragments (scaffolds) represents a potential way to enhance the immune response to these specific epitopes.
27449907	1	8	theme	HIV-1	154:158	arg1	gp120					177:181	the HIV-1 envelope protein gp120	150:181	the HIV-1 envelope protein gp120	150:181	The V1/V2 domain of the HIV-1 envelope protein gp120 possesses two important epitopes: a glycan-dependent epitope recognized by the prototypic broadly neutralizing monoclonal antibody (bN-mAb), PG9, as well as an epitope recognized by non-neutralizing antibodies that has been associated with protection from HIV infection in the RV144 HIV vaccine trial.
27449907	4	9	theme	diverse	1098:1104	arg1	several					1126:1132	several	1126:1132	several	1126:1132	In this paper, we used a protein engineering approach to improve the conformational stability and antibody binding activity of V1/V2 domain scaffolds from multiple diverse isolates, including several that were initially unable to bind the prototypic PG9 bN-mAb.
27449907	4	9	theme	diverse	1098:1104	arg1	isolates					1106:1113	multiple diverse isolates	1089:1113	multiple diverse isolates	1089:1113	In this paper, we used a protein engineering approach to improve the conformational stability and antibody binding activity of V1/V2 domain scaffolds from multiple diverse isolates, including several that were initially unable to bind the prototypic PG9 bN-mAb.
27449907	2	10	with	immunization	588:599	arg1	scaffolds					650:658	scaffolds	650:658	scaffolds	650:658	Because both of these epitopes are poorly immunogenic in the context of full length envelope proteins, immunization with properly folded and glycosylated fragments (scaffolds) represents a potential way to enhance the immune response to these specific epitopes.
27449907	2	10	with	immunization	588:599	arg1	fragments					639:647	properly folded and glycosylated fragments	606:647	properly folded and glycosylated fragments (scaffolds)	606:659	Because both of these epitopes are poorly immunogenic in the context of full length envelope proteins, immunization with properly folded and glycosylated fragments (scaffolds) represents a potential way to enhance the immune response to these specific epitopes.
27449907	1	11	theme	protein	169:175	arg1	gp120					177:181	the HIV-1 envelope protein gp120	150:181	the HIV-1 envelope protein gp120	150:181	The V1/V2 domain of the HIV-1 envelope protein gp120 possesses two important epitopes: a glycan-dependent epitope recognized by the prototypic broadly neutralizing monoclonal antibody (bN-mAb), PG9, as well as an epitope recognized by non-neutralizing antibodies that has been associated with protection from HIV infection in the RV144 HIV vaccine trial.
27449907	7	12	theme	glycopeptide	1676:1687	arg1	scaffolds					1689:1697	engineered glycopeptide scaffolds	1665:1697	engineered glycopeptide scaffolds from three different clades of HIV-1 that bind PG9 with high affinity when expressed in a wildtype cell line suitable for biopharmaceutical production	1665:1848	In this report, we describe engineered glycopeptide scaffolds from three different clades of HIV-1 that bind PG9 with high affinity when expressed in a wildtype cell line suitable for biopharmaceutical production.
27449907	9	13	theme	HIV	2108:2110	arg1	development					2120:2130	HIV vaccine development	2108:2130	HIV vaccine development	2108:2130	The scaffolds produced address three major problems in HIV vaccine development: (1) improving antibody responses to poorly immunogenic epitopes in the V1/V2 domain; (2) eliminating antibody responses to highly immunogenic (decoy) epitopes outside the V1/V2 domain; and (3) enabling the production of V1/V2 scaffolds in a cell line suitable for biopharmaceutical production.
27449907	0	14	theme	neutralizing	103:114	arg1	antibody					120:127	the broadly neutralizing PG9 antibody	91:127	the broadly neutralizing PG9 antibody	91:127	Fragments of the V1/V2 domain of HIV-1 glycoprotein 120 engineered for improved binding to the broadly neutralizing PG9 antibody.
27449907	4	15	theme	domain	1067:1072	arg1	scaffolds					1074:1082	V1/V2 domain scaffolds	1061:1082	V1/V2 domain scaffolds from multiple diverse isolates, including several that were initially unable to bind the prototypic PG9 bN-mAb	1061:1193	In this paper, we used a protein engineering approach to improve the conformational stability and antibody binding activity of V1/V2 domain scaffolds from multiple diverse isolates, including several that were initially unable to bind the prototypic PG9 bN-mAb.
27449907	1	16	theme	non-neutralizing	365:380	arg1	antibodies					382:391	non-neutralizing antibodies	365:391	non-neutralizing antibodies that has been associated with protection from HIV infection in the RV144 HIV vaccine trial	365:482	The V1/V2 domain of the HIV-1 envelope protein gp120 possesses two important epitopes: a glycan-dependent epitope recognized by the prototypic broadly neutralizing monoclonal antibody (bN-mAb), PG9, as well as an epitope recognized by non-neutralizing antibodies that has been associated with protection from HIV infection in the RV144 HIV vaccine trial.
27449907	1	17	theme	important	197:205	arg1	epitopes					207:214	two important epitopes	193:214	two important epitopes: a glycan-dependent epitope recognized by the prototypic broadly neutralizing monoclonal antibody (bN-mAb), PG9, as well as an epitope recognized by non-neutralizing antibodies that has been associated with protection from HIV infection in the RV144 HIV vaccine trial	193:482	The V1/V2 domain of the HIV-1 envelope protein gp120 possesses two important epitopes: a glycan-dependent epitope recognized by the prototypic broadly neutralizing monoclonal antibody (bN-mAb), PG9, as well as an epitope recognized by non-neutralizing antibodies that has been associated with protection from HIV infection in the RV144 HIV vaccine trial.
27449907	1	17	theme	important	197:205	arg1	PG9					324:326	PG9	324:326	PG9	324:326	The V1/V2 domain of the HIV-1 envelope protein gp120 possesses two important epitopes: a glycan-dependent epitope recognized by the prototypic broadly neutralizing monoclonal antibody (bN-mAb), PG9, as well as an epitope recognized by non-neutralizing antibodies that has been associated with protection from HIV infection in the RV144 HIV vaccine trial.
27449907	1	17	theme	important	197:205	arg1	epitope					236:242	a glycan-dependent epitope	217:242	a glycan-dependent epitope recognized by the prototypic broadly neutralizing monoclonal antibody (bN-mAb)	217:321	The V1/V2 domain of the HIV-1 envelope protein gp120 possesses two important epitopes: a glycan-dependent epitope recognized by the prototypic broadly neutralizing monoclonal antibody (bN-mAb), PG9, as well as an epitope recognized by non-neutralizing antibodies that has been associated with protection from HIV infection in the RV144 HIV vaccine trial.
27449907	1	17	theme	important	197:205	arg1	epitope					343:349	an epitope	340:349	an epitope recognized by non-neutralizing antibodies that has been associated with protection from HIV infection in the RV144 HIV vaccine trial	340:482	The V1/V2 domain of the HIV-1 envelope protein gp120 possesses two important epitopes: a glycan-dependent epitope recognized by the prototypic broadly neutralizing monoclonal antibody (bN-mAb), PG9, as well as an epitope recognized by non-neutralizing antibodies that has been associated with protection from HIV infection in the RV144 HIV vaccine trial.
27449907	6	18	theme	proteins	1598:1605	arg1	production					1584:1593	biopharmaceutical production	1566:1593	biopharmaceutical production of proteins intended for clinical trials	1566:1634	Although scaffolds incorporating the glycans required for PG9 binding (e.g., mannose-5) can be produced using glycosylation inhibitors (e.g., swainsonine), or mutant cell lines (e.g. GnTI(-) 293 HEK), these are not practical for biopharmaceutical production of proteins intended for clinical trials.
27449907	9	19	from	responses	2156:2164	arg1	domain					2210:2215	the V1/V2 domain	2200:2215	the V1/V2 domain	2200:2215	The scaffolds produced address three major problems in HIV vaccine development: (1) improving antibody responses to poorly immunogenic epitopes in the V1/V2 domain; (2) eliminating antibody responses to highly immunogenic (decoy) epitopes outside the V1/V2 domain; and (3) enabling the production of V1/V2 scaffolds in a cell line suitable for biopharmaceutical production.
27449907	3	20	theme	selected	828:835	arg1	isolates					837:844	a few selected isolates	822:844	a few selected isolates	822:844	Previous studies showed that V1/V2 domain scaffolds could be produced from a few selected isolates, but not from many of the isolates that would be advantageous in a multivalent vaccine.
27449907	3	21	theme	domain	782:787	arg1	scaffolds					789:797	V1/V2 domain scaffolds	776:797	V1/V2 domain scaffolds	776:797	Previous studies showed that V1/V2 domain scaffolds could be produced from a few selected isolates, but not from many of the isolates that would be advantageous in a multivalent vaccine.
27449907	2	22	theme	immune	703:708	arg1	response					710:717	the immune response	699:717	the immune response to these specific epitopes	699:744	Because both of these epitopes are poorly immunogenic in the context of full length envelope proteins, immunization with properly folded and glycosylated fragments (scaffolds) represents a potential way to enhance the immune response to these specific epitopes.
27449907	5	23	theme	glycan	1261:1266	arg1	structure					1268:1276	the correct glycan structure	1249:1276	the correct glycan structure	1249:1276	Significantly, this effort required replicating both the correct glycan structure as well as the β-sheet structure required for PG9 binding.
27449907	9	24	theme	antibody	2147:2154	arg1	responses					2156:2164	antibody responses	2147:2164	antibody responses to poorly immunogenic epitopes in the V1/V2 domain; (2) eliminating antibody responses to highly immunogenic (decoy) epitopes outside the V1/V2 domain	2147:2315	The scaffolds produced address three major problems in HIV vaccine development: (1) improving antibody responses to poorly immunogenic epitopes in the V1/V2 domain; (2) eliminating antibody responses to highly immunogenic (decoy) epitopes outside the V1/V2 domain; and (3) enabling the production of V1/V2 scaffolds in a cell line suitable for biopharmaceutical production.
27449907	9	25	theme	V1/V2	2353:2357	arg1	scaffolds					2359:2367	V1/V2 scaffolds	2353:2367	V1/V2 scaffolds	2353:2367	The scaffolds produced address three major problems in HIV vaccine development: (1) improving antibody responses to poorly immunogenic epitopes in the V1/V2 domain; (2) eliminating antibody responses to highly immunogenic (decoy) epitopes outside the V1/V2 domain; and (3) enabling the production of V1/V2 scaffolds in a cell line suitable for biopharmaceutical production.
27449907	1	26	theme	V1/V2	134:138	arg1	domain					140:145	The V1/V2 domain	130:145	The V1/V2 domain of the HIV-1 envelope protein gp120	130:181	The V1/V2 domain of the HIV-1 envelope protein gp120 possesses two important epitopes: a glycan-dependent epitope recognized by the prototypic broadly neutralizing monoclonal antibody (bN-mAb), PG9, as well as an epitope recognized by non-neutralizing antibodies that has been associated with protection from HIV infection in the RV144 HIV vaccine trial.
27449907	1	26	theme	V1/V2	134:138	arg1	gp120					177:181	the HIV-1 envelope protein gp120	150:181	the HIV-1 envelope protein gp120	150:181	The V1/V2 domain of the HIV-1 envelope protein gp120 possesses two important epitopes: a glycan-dependent epitope recognized by the prototypic broadly neutralizing monoclonal antibody (bN-mAb), PG9, as well as an epitope recognized by non-neutralizing antibodies that has been associated with protection from HIV infection in the RV144 HIV vaccine trial.
27449907	4	27	dep	stability	1018:1026	arg1	the					999:1001	the	999:1001	the	999:1001	In this paper, we used a protein engineering approach to improve the conformational stability and antibody binding activity of V1/V2 domain scaffolds from multiple diverse isolates, including several that were initially unable to bind the prototypic PG9 bN-mAb.
27449907	2	28	theme	glycosylated	626:637	arg1	scaffolds					650:658	scaffolds	650:658	scaffolds	650:658	Because both of these epitopes are poorly immunogenic in the context of full length envelope proteins, immunization with properly folded and glycosylated fragments (scaffolds) represents a potential way to enhance the immune response to these specific epitopes.
27449907	2	28	theme	glycosylated	626:637	arg1	fragments					639:647	properly folded and glycosylated fragments	606:647	properly folded and glycosylated fragments (scaffolds)	606:659	Because both of these epitopes are poorly immunogenic in the context of full length envelope proteins, immunization with properly folded and glycosylated fragments (scaffolds) represents a potential way to enhance the immune response to these specific epitopes.
27449907	9	29	theme	immunogenic	2176:2186	arg1	epitopes					2188:2195	poorly immunogenic epitopes	2169:2195	poorly immunogenic epitopes	2169:2195	The scaffolds produced address three major problems in HIV vaccine development: (1) improving antibody responses to poorly immunogenic epitopes in the V1/V2 domain; (2) eliminating antibody responses to highly immunogenic (decoy) epitopes outside the V1/V2 domain; and (3) enabling the production of V1/V2 scaffolds in a cell line suitable for biopharmaceutical production.
27449907	4	30	used	used	952:955	arg2	we					949:950	we	949:950	we	949:950	In this paper, we used a protein engineering approach to improve the conformational stability and antibody binding activity of V1/V2 domain scaffolds from multiple diverse isolates, including several that were initially unable to bind the prototypic PG9 bN-mAb.
27449907	9	31	theme	cell	2374:2377	arg1	line					2379:2382	a cell line	2372:2382	a cell line suitable for biopharmaceutical production	2372:2424	The scaffolds produced address three major problems in HIV vaccine development: (1) improving antibody responses to poorly immunogenic epitopes in the V1/V2 domain; (2) eliminating antibody responses to highly immunogenic (decoy) epitopes outside the V1/V2 domain; and (3) enabling the production of V1/V2 scaffolds in a cell line suitable for biopharmaceutical production.
27449907	2	32	theme	folded	615:620	arg1	scaffolds					650:658	scaffolds	650:658	scaffolds	650:658	Because both of these epitopes are poorly immunogenic in the context of full length envelope proteins, immunization with properly folded and glycosylated fragments (scaffolds) represents a potential way to enhance the immune response to these specific epitopes.
27449907	2	32	theme	folded	615:620	arg1	fragments					639:647	properly folded and glycosylated fragments	606:647	properly folded and glycosylated fragments (scaffolds)	606:659	Because both of these epitopes are poorly immunogenic in the context of full length envelope proteins, immunization with properly folded and glycosylated fragments (scaffolds) represents a potential way to enhance the immune response to these specific epitopes.
27449907	5	33	theme	β-sheet	1293:1299	arg1	structure					1301:1309	the β-sheet structure	1289:1309	the β-sheet structure required for PG9 binding	1289:1334	Significantly, this effort required replicating both the correct glycan structure as well as the β-sheet structure required for PG9 binding.
27449907	9	34	theme	suitable	2384:2391	arg1	line					2379:2382	a cell line	2372:2382	a cell line suitable for biopharmaceutical production	2372:2424	The scaffolds produced address three major problems in HIV vaccine development: (1) improving antibody responses to poorly immunogenic epitopes in the V1/V2 domain; (2) eliminating antibody responses to highly immunogenic (decoy) epitopes outside the V1/V2 domain; and (3) enabling the production of V1/V2 scaffolds in a cell line suitable for biopharmaceutical production.
27449907	4	35	theme	engineering	967:977	arg1	approach					979:986	a protein engineering approach	957:986	a protein engineering approach	957:986	In this paper, we used a protein engineering approach to improve the conformational stability and antibody binding activity of V1/V2 domain scaffolds from multiple diverse isolates, including several that were initially unable to bind the prototypic PG9 bN-mAb.
27449907	9	36	theme	V1/V2	2204:2208	arg1	domain					2210:2215	the V1/V2 domain	2200:2215	the V1/V2 domain	2200:2215	The scaffolds produced address three major problems in HIV vaccine development: (1) improving antibody responses to poorly immunogenic epitopes in the V1/V2 domain; (2) eliminating antibody responses to highly immunogenic (decoy) epitopes outside the V1/V2 domain; and (3) enabling the production of V1/V2 scaffolds in a cell line suitable for biopharmaceutical production.
27449907	8	37	theme	amino	1938:1942	arg1	positions					1949:1957	amino acid positions	1938:1957	amino acid positions outside of the antibody contact region designed to stabilize the β-sheet and turn structures	1938:2050	The mutations that improved PG9 binding to scaffolds produced in normal cells included amino acid positions outside of the antibody contact region designed to stabilize the β-sheet and turn structures.
27449907	1	38	theme	prototypic	262:271	arg1	bN-mAb					315:320	bN-mAb	315:320	bN-mAb	315:320	The V1/V2 domain of the HIV-1 envelope protein gp120 possesses two important epitopes: a glycan-dependent epitope recognized by the prototypic broadly neutralizing monoclonal antibody (bN-mAb), PG9, as well as an epitope recognized by non-neutralizing antibodies that has been associated with protection from HIV infection in the RV144 HIV vaccine trial.
27449907	1	38	theme	prototypic	262:271	arg1	antibody					305:312	the prototypic broadly neutralizing monoclonal antibody	258:312	the prototypic broadly neutralizing monoclonal antibody (bN-mAb)	258:321	The V1/V2 domain of the HIV-1 envelope protein gp120 possesses two important epitopes: a glycan-dependent epitope recognized by the prototypic broadly neutralizing monoclonal antibody (bN-mAb), PG9, as well as an epitope recognized by non-neutralizing antibodies that has been associated with protection from HIV infection in the RV144 HIV vaccine trial.
27449907	9	39	theme	biopharmaceutical	2397:2413	arg1	production					2415:2424	biopharmaceutical production	2397:2424	biopharmaceutical production	2397:2424	The scaffolds produced address three major problems in HIV vaccine development: (1) improving antibody responses to poorly immunogenic epitopes in the V1/V2 domain; (2) eliminating antibody responses to highly immunogenic (decoy) epitopes outside the V1/V2 domain; and (3) enabling the production of V1/V2 scaffolds in a cell line suitable for biopharmaceutical production.
27449907	4	40	theme	prototypic	1173:1182	arg1	bN-mAb					1188:1193	the prototypic PG9 bN-mAb	1169:1193	the prototypic PG9 bN-mAb	1169:1193	In this paper, we used a protein engineering approach to improve the conformational stability and antibody binding activity of V1/V2 domain scaffolds from multiple diverse isolates, including several that were initially unable to bind the prototypic PG9 bN-mAb.
27449907	1	41	theme	neutralizing	281:292	arg1	bN-mAb					315:320	bN-mAb	315:320	bN-mAb	315:320	The V1/V2 domain of the HIV-1 envelope protein gp120 possesses two important epitopes: a glycan-dependent epitope recognized by the prototypic broadly neutralizing monoclonal antibody (bN-mAb), PG9, as well as an epitope recognized by non-neutralizing antibodies that has been associated with protection from HIV infection in the RV144 HIV vaccine trial.
27449907	1	41	theme	neutralizing	281:292	arg1	antibody					305:312	the prototypic broadly neutralizing monoclonal antibody	258:312	the prototypic broadly neutralizing monoclonal antibody (bN-mAb)	258:321	The V1/V2 domain of the HIV-1 envelope protein gp120 possesses two important epitopes: a glycan-dependent epitope recognized by the prototypic broadly neutralizing monoclonal antibody (bN-mAb), PG9, as well as an epitope recognized by non-neutralizing antibodies that has been associated with protection from HIV infection in the RV144 HIV vaccine trial.
27449907	0	42	theme	domain	23:28	arg1	Fragments					0:8	Fragments	0:8	Fragments of the V1/V2 domain of HIV-1 glycoprotein 120	0:54	Fragments of the V1/V2 domain of HIV-1 glycoprotein 120 engineered for improved binding to the broadly neutralizing PG9 antibody.
27449907	3	43	theme	multivalent	913:923	arg1	vaccine					925:931	a multivalent vaccine	911:931	a multivalent vaccine	911:931	Previous studies showed that V1/V2 domain scaffolds could be produced from a few selected isolates, but not from many of the isolates that would be advantageous in a multivalent vaccine.
27449907	3	44	from	vaccine	925:931	arg1	isolates					872:879	the isolates	868:879	the isolates that would be advantageous in a multivalent vaccine	868:931	Previous studies showed that V1/V2 domain scaffolds could be produced from a few selected isolates, but not from many of the isolates that would be advantageous in a multivalent vaccine.
27449907	3	44	from	vaccine	925:931	arg1	advantageous					895:906	advantageous	895:906	advantageous	895:906	Previous studies showed that V1/V2 domain scaffolds could be produced from a few selected isolates, but not from many of the isolates that would be advantageous in a multivalent vaccine.
27449907	1	45	theme	vaccine	470:476	arg1	trial					478:482	the RV144 HIV vaccine trial	456:482	the RV144 HIV vaccine trial	456:482	The V1/V2 domain of the HIV-1 envelope protein gp120 possesses two important epitopes: a glycan-dependent epitope recognized by the prototypic broadly neutralizing monoclonal antibody (bN-mAb), PG9, as well as an epitope recognized by non-neutralizing antibodies that has been associated with protection from HIV infection in the RV144 HIV vaccine trial.
27449907	9	46	theme	eliminating	2222:2232	arg1	responses					2243:2251	antibody responses	2234:2251	antibody responses to poorly immunogenic epitopes in the V1/V2 domain; (2) eliminating antibody responses to highly immunogenic (decoy) epitopes outside the V1/V2 domain	2147:2315	The scaffolds produced address three major problems in HIV vaccine development: (1) improving antibody responses to poorly immunogenic epitopes in the V1/V2 domain; (2) eliminating antibody responses to highly immunogenic (decoy) epitopes outside the V1/V2 domain; and (3) enabling the production of V1/V2 scaffolds in a cell line suitable for biopharmaceutical production.
27449907	0	47	theme	HIV-1	33:37	arg1	glycoprotein					39:50	HIV-1 glycoprotein 120	33:54	HIV-1 glycoprotein 120	33:54	Fragments of the V1/V2 domain of HIV-1 glycoprotein 120 engineered for improved binding to the broadly neutralizing PG9 antibody.
27449907	7	48	theme	wildtype	1789:1796	arg1	line					1803:1806	a wildtype cell line	1787:1806	a wildtype cell line suitable for biopharmaceutical production	1787:1848	In this report, we describe engineered glycopeptide scaffolds from three different clades of HIV-1 that bind PG9 with high affinity when expressed in a wildtype cell line suitable for biopharmaceutical production.
27449907	4	49	theme	antibody	1032:1039	arg1	activity					1049:1056	antibody binding activity	1032:1056	antibody binding activity	1032:1056	In this paper, we used a protein engineering approach to improve the conformational stability and antibody binding activity of V1/V2 domain scaffolds from multiple diverse isolates, including several that were initially unable to bind the prototypic PG9 bN-mAb.
27449907	6	50	theme	glycosylation	1447:1459	arg1	inhibitors					1461:1470	glycosylation inhibitors	1447:1470	glycosylation inhibitors (e.g., swainsonine)	1447:1490	Although scaffolds incorporating the glycans required for PG9 binding (e.g., mannose-5) can be produced using glycosylation inhibitors (e.g., swainsonine), or mutant cell lines (e.g. GnTI(-) 293 HEK), these are not practical for biopharmaceutical production of proteins intended for clinical trials.
27449907	5	51	theme	PG9	1324:1326	arg1	binding					1328:1334	PG9 binding	1324:1334	PG9 binding	1324:1334	Significantly, this effort required replicating both the correct glycan structure as well as the β-sheet structure required for PG9 binding.
27449907	8	52	theme	contact	1983:1989	arg1	region					1991:1996	the antibody contact region	1970:1996	the antibody contact region designed to stabilize the β-sheet and turn structures	1970:2050	The mutations that improved PG9 binding to scaffolds produced in normal cells included amino acid positions outside of the antibody contact region designed to stabilize the β-sheet and turn structures.
27449907	0	53	gly	glycoprotein	39:50	arg1	glycoprotein					39:50	HIV-1 glycoprotein 120	33:54	HIV-1 glycoprotein 120	33:54	Fragments of the V1/V2 domain of HIV-1 glycoprotein 120 engineered for improved binding to the broadly neutralizing PG9 antibody.
27449907	7	54	theme	HIV-1	1730:1734	arg1	clades					1720:1725	three different clades	1704:1725	three different clades of HIV-1	1704:1734	In this report, we describe engineered glycopeptide scaffolds from three different clades of HIV-1 that bind PG9 with high affinity when expressed in a wildtype cell line suitable for biopharmaceutical production.
27449907	2	55	gly	glycosylated	626:637	arg1	scaffolds					650:658	scaffolds	650:658	scaffolds	650:658	Because both of these epitopes are poorly immunogenic in the context of full length envelope proteins, immunization with properly folded and glycosylated fragments (scaffolds) represents a potential way to enhance the immune response to these specific epitopes.
27449907	2	55	gly	glycosylated	626:637	arg1	fragments					639:647	properly folded and glycosylated fragments	606:647	properly folded and glycosylated fragments (scaffolds)	606:659	Because both of these epitopes are poorly immunogenic in the context of full length envelope proteins, immunization with properly folded and glycosylated fragments (scaffolds) represents a potential way to enhance the immune response to these specific epitopes.
27449907	2	56	theme	envelope	569:576	arg1	proteins					578:585	full length envelope proteins	557:585	full length envelope proteins	557:585	Because both of these epitopes are poorly immunogenic in the context of full length envelope proteins, immunization with properly folded and glycosylated fragments (scaffolds) represents a potential way to enhance the immune response to these specific epitopes.
27449907	2	57	theme	full	557:560	arg1	proteins					578:585	full length envelope proteins	557:585	full length envelope proteins	557:585	Because both of these epitopes are poorly immunogenic in the context of full length envelope proteins, immunization with properly folded and glycosylated fragments (scaffolds) represents a potential way to enhance the immune response to these specific epitopes.
27449907	6	58	dep	swainsonine	1479:1489	arg1	e.g.					1473:1476	e.g.	1473:1476	e.g.	1473:1476	Although scaffolds incorporating the glycans required for PG9 binding (e.g., mannose-5) can be produced using glycosylation inhibitors (e.g., swainsonine), or mutant cell lines (e.g. GnTI(-) 293 HEK), these are not practical for biopharmaceutical production of proteins intended for clinical trials.
27449907	4	59	theme	multiple	1089:1096	arg1	several					1126:1132	several	1126:1132	several	1126:1132	In this paper, we used a protein engineering approach to improve the conformational stability and antibody binding activity of V1/V2 domain scaffolds from multiple diverse isolates, including several that were initially unable to bind the prototypic PG9 bN-mAb.
27449907	4	59	theme	multiple	1089:1096	arg1	isolates					1106:1113	multiple diverse isolates	1089:1113	multiple diverse isolates	1089:1113	In this paper, we used a protein engineering approach to improve the conformational stability and antibody binding activity of V1/V2 domain scaffolds from multiple diverse isolates, including several that were initially unable to bind the prototypic PG9 bN-mAb.
27449907	2	60	from	context	546:552	arg1	immunogenic					527:537	immunogenic	527:537	immunogenic	527:537	Because both of these epitopes are poorly immunogenic in the context of full length envelope proteins, immunization with properly folded and glycosylated fragments (scaffolds) represents a potential way to enhance the immune response to these specific epitopes.
27449907	1	61	theme	HIV	439:441	arg1	infection					443:451	HIV infection	439:451	HIV infection in the RV144 HIV vaccine trial	439:482	The V1/V2 domain of the HIV-1 envelope protein gp120 possesses two important epitopes: a glycan-dependent epitope recognized by the prototypic broadly neutralizing monoclonal antibody (bN-mAb), PG9, as well as an epitope recognized by non-neutralizing antibodies that has been associated with protection from HIV infection in the RV144 HIV vaccine trial.
27449907	4	62	from	isolates	1106:1113	arg1	stability					1018:1026	conformational stability	1003:1026	conformational stability	1003:1026	In this paper, we used a protein engineering approach to improve the conformational stability and antibody binding activity of V1/V2 domain scaffolds from multiple diverse isolates, including several that were initially unable to bind the prototypic PG9 bN-mAb.
27449907	4	62	from	isolates	1106:1113	arg1	scaffolds					1074:1082	V1/V2 domain scaffolds	1061:1082	V1/V2 domain scaffolds from multiple diverse isolates, including several that were initially unable to bind the prototypic PG9 bN-mAb	1061:1193	In this paper, we used a protein engineering approach to improve the conformational stability and antibody binding activity of V1/V2 domain scaffolds from multiple diverse isolates, including several that were initially unable to bind the prototypic PG9 bN-mAb.
27449907	4	62	from	isolates	1106:1113	arg1	activity					1049:1056	antibody binding activity	1032:1056	antibody binding activity	1032:1056	In this paper, we used a protein engineering approach to improve the conformational stability and antibody binding activity of V1/V2 domain scaffolds from multiple diverse isolates, including several that were initially unable to bind the prototypic PG9 bN-mAb.
27449907	8	63	theme	antibody	1974:1981	arg1	region					1991:1996	the antibody contact region	1970:1996	the antibody contact region designed to stabilize the β-sheet and turn structures	1970:2050	The mutations that improved PG9 binding to scaffolds produced in normal cells included amino acid positions outside of the antibody contact region designed to stabilize the β-sheet and turn structures.
27449907	9	64	theme	major	2090:2094	arg1	problems					2096:2103	three major problems	2084:2103	three major problems in HIV vaccine development	2084:2130	The scaffolds produced address three major problems in HIV vaccine development: (1) improving antibody responses to poorly immunogenic epitopes in the V1/V2 domain; (2) eliminating antibody responses to highly immunogenic (decoy) epitopes outside the V1/V2 domain; and (3) enabling the production of V1/V2 scaffolds in a cell line suitable for biopharmaceutical production.
27449907	9	65	from	problems	2096:2103	arg1	development					2120:2130	HIV vaccine development	2108:2130	HIV vaccine development	2108:2130	The scaffolds produced address three major problems in HIV vaccine development: (1) improving antibody responses to poorly immunogenic epitopes in the V1/V2 domain; (2) eliminating antibody responses to highly immunogenic (decoy) epitopes outside the V1/V2 domain; and (3) enabling the production of V1/V2 scaffolds in a cell line suitable for biopharmaceutical production.
27449907	9	66	theme	V1/V2	2304:2308	arg1	domain					2310:2315	the V1/V2 domain	2300:2315	the V1/V2 domain	2300:2315	The scaffolds produced address three major problems in HIV vaccine development: (1) improving antibody responses to poorly immunogenic epitopes in the V1/V2 domain; (2) eliminating antibody responses to highly immunogenic (decoy) epitopes outside the V1/V2 domain; and (3) enabling the production of V1/V2 scaffolds in a cell line suitable for biopharmaceutical production.
27449907	9	67	from	production	2339:2348	arg1	line					2379:2382	a cell line	2372:2382	a cell line suitable for biopharmaceutical production	2372:2424	The scaffolds produced address three major problems in HIV vaccine development: (1) improving antibody responses to poorly immunogenic epitopes in the V1/V2 domain; (2) eliminating antibody responses to highly immunogenic (decoy) epitopes outside the V1/V2 domain; and (3) enabling the production of V1/V2 scaffolds in a cell line suitable for biopharmaceutical production.
27449907	1	68	theme	envelope	160:167	arg1	gp120					177:181	the HIV-1 envelope protein gp120	150:181	the HIV-1 envelope protein gp120	150:181	The V1/V2 domain of the HIV-1 envelope protein gp120 possesses two important epitopes: a glycan-dependent epitope recognized by the prototypic broadly neutralizing monoclonal antibody (bN-mAb), PG9, as well as an epitope recognized by non-neutralizing antibodies that has been associated with protection from HIV infection in the RV144 HIV vaccine trial.
27449907	7	69	theme	engineered	1665:1674	arg1	scaffolds					1689:1697	engineered glycopeptide scaffolds	1665:1697	engineered glycopeptide scaffolds from three different clades of HIV-1 that bind PG9 with high affinity when expressed in a wildtype cell line suitable for biopharmaceutical production	1665:1848	In this report, we describe engineered glycopeptide scaffolds from three different clades of HIV-1 that bind PG9 with high affinity when expressed in a wildtype cell line suitable for biopharmaceutical production.
27449907	4	70	theme	V1/V2	1061:1065	arg1	scaffolds					1074:1082	V1/V2 domain scaffolds	1061:1082	V1/V2 domain scaffolds from multiple diverse isolates, including several that were initially unable to bind the prototypic PG9 bN-mAb	1061:1193	In this paper, we used a protein engineering approach to improve the conformational stability and antibody binding activity of V1/V2 domain scaffolds from multiple diverse isolates, including several that were initially unable to bind the prototypic PG9 bN-mAb.
27449907	0	71	theme	PG9	116:118	arg1	antibody					120:127	the broadly neutralizing PG9 antibody	91:127	the broadly neutralizing PG9 antibody	91:127	Fragments of the V1/V2 domain of HIV-1 glycoprotein 120 engineered for improved binding to the broadly neutralizing PG9 antibody.
27449907	1	72	theme	RV144	460:464	arg1	trial					478:482	the RV144 HIV vaccine trial	456:482	the RV144 HIV vaccine trial	456:482	The V1/V2 domain of the HIV-1 envelope protein gp120 possesses two important epitopes: a glycan-dependent epitope recognized by the prototypic broadly neutralizing monoclonal antibody (bN-mAb), PG9, as well as an epitope recognized by non-neutralizing antibodies that has been associated with protection from HIV infection in the RV144 HIV vaccine trial.
27449907	1	73	theme	gp120	177:181	arg1	domain					140:145	The V1/V2 domain	130:145	The V1/V2 domain of the HIV-1 envelope protein gp120	130:181	The V1/V2 domain of the HIV-1 envelope protein gp120 possesses two important epitopes: a glycan-dependent epitope recognized by the prototypic broadly neutralizing monoclonal antibody (bN-mAb), PG9, as well as an epitope recognized by non-neutralizing antibodies that has been associated with protection from HIV infection in the RV144 HIV vaccine trial.
27449907	1	73	theme	gp120	177:181	arg1	gp120					177:181	the HIV-1 envelope protein gp120	150:181	the HIV-1 envelope protein gp120	150:181	The V1/V2 domain of the HIV-1 envelope protein gp120 possesses two important epitopes: a glycan-dependent epitope recognized by the prototypic broadly neutralizing monoclonal antibody (bN-mAb), PG9, as well as an epitope recognized by non-neutralizing antibodies that has been associated with protection from HIV infection in the RV144 HIV vaccine trial.
27449907	6	74	theme	clinical	1620:1627	arg1	trials					1629:1634	clinical trials	1620:1634	clinical trials	1620:1634	Although scaffolds incorporating the glycans required for PG9 binding (e.g., mannose-5) can be produced using glycosylation inhibitors (e.g., swainsonine), or mutant cell lines (e.g. GnTI(-) 293 HEK), these are not practical for biopharmaceutical production of proteins intended for clinical trials.
27449907	9	75	dep	immunogenic	2263:2273	arg1	decoy					2276:2280	decoy	2276:2280	decoy	2276:2280	The scaffolds produced address three major problems in HIV vaccine development: (1) improving antibody responses to poorly immunogenic epitopes in the V1/V2 domain; (2) eliminating antibody responses to highly immunogenic (decoy) epitopes outside the V1/V2 domain; and (3) enabling the production of V1/V2 scaffolds in a cell line suitable for biopharmaceutical production.
27449907	4	76	theme	scaffolds	1074:1082	arg1	stability					1018:1026	conformational stability	1003:1026	conformational stability	1003:1026	In this paper, we used a protein engineering approach to improve the conformational stability and antibody binding activity of V1/V2 domain scaffolds from multiple diverse isolates, including several that were initially unable to bind the prototypic PG9 bN-mAb.
27449907	4	76	theme	scaffolds	1074:1082	arg1	activity					1049:1056	antibody binding activity	1032:1056	antibody binding activity	1032:1056	In this paper, we used a protein engineering approach to improve the conformational stability and antibody binding activity of V1/V2 domain scaffolds from multiple diverse isolates, including several that were initially unable to bind the prototypic PG9 bN-mAb.
27449907	9	77	theme	vaccine	2112:2118	arg1	development					2120:2130	HIV vaccine development	2108:2130	HIV vaccine development	2108:2130	The scaffolds produced address three major problems in HIV vaccine development: (1) improving antibody responses to poorly immunogenic epitopes in the V1/V2 domain; (2) eliminating antibody responses to highly immunogenic (decoy) epitopes outside the V1/V2 domain; and (3) enabling the production of V1/V2 scaffolds in a cell line suitable for biopharmaceutical production.
27449907	1	78	dep	epitopes	207:214	arg1	epitopes					207:214	two important epitopes	193:214	two important epitopes: a glycan-dependent epitope recognized by the prototypic broadly neutralizing monoclonal antibody (bN-mAb), PG9, as well as an epitope recognized by non-neutralizing antibodies that has been associated with protection from HIV infection in the RV144 HIV vaccine trial	193:482	The V1/V2 domain of the HIV-1 envelope protein gp120 possesses two important epitopes: a glycan-dependent epitope recognized by the prototypic broadly neutralizing monoclonal antibody (bN-mAb), PG9, as well as an epitope recognized by non-neutralizing antibodies that has been associated with protection from HIV infection in the RV144 HIV vaccine trial.
27449907	1	78	dep	epitopes	207:214	arg1	PG9					324:326	PG9	324:326	PG9	324:326	The V1/V2 domain of the HIV-1 envelope protein gp120 possesses two important epitopes: a glycan-dependent epitope recognized by the prototypic broadly neutralizing monoclonal antibody (bN-mAb), PG9, as well as an epitope recognized by non-neutralizing antibodies that has been associated with protection from HIV infection in the RV144 HIV vaccine trial.
27449907	1	78	dep	epitopes	207:214	arg1	epitope					236:242	a glycan-dependent epitope	217:242	a glycan-dependent epitope recognized by the prototypic broadly neutralizing monoclonal antibody (bN-mAb)	217:321	The V1/V2 domain of the HIV-1 envelope protein gp120 possesses two important epitopes: a glycan-dependent epitope recognized by the prototypic broadly neutralizing monoclonal antibody (bN-mAb), PG9, as well as an epitope recognized by non-neutralizing antibodies that has been associated with protection from HIV infection in the RV144 HIV vaccine trial.
27449907	1	78	dep	epitopes	207:214	arg1	epitope					343:349	an epitope	340:349	an epitope recognized by non-neutralizing antibodies that has been associated with protection from HIV infection in the RV144 HIV vaccine trial	340:482	The V1/V2 domain of the HIV-1 envelope protein gp120 possesses two important epitopes: a glycan-dependent epitope recognized by the prototypic broadly neutralizing monoclonal antibody (bN-mAb), PG9, as well as an epitope recognized by non-neutralizing antibodies that has been associated with protection from HIV infection in the RV144 HIV vaccine trial.
27449907	4	79	from	activity	1049:1056	arg1	several					1126:1132	several	1126:1132	several	1126:1132	In this paper, we used a protein engineering approach to improve the conformational stability and antibody binding activity of V1/V2 domain scaffolds from multiple diverse isolates, including several that were initially unable to bind the prototypic PG9 bN-mAb.
27449907	4	79	from	activity	1049:1056	arg1	isolates					1106:1113	multiple diverse isolates	1089:1113	multiple diverse isolates	1089:1113	In this paper, we used a protein engineering approach to improve the conformational stability and antibody binding activity of V1/V2 domain scaffolds from multiple diverse isolates, including several that were initially unable to bind the prototypic PG9 bN-mAb.
27449907	9	80	dep	produced	2067:2074	arg1	enabling					2326:2333	enabling	2326:2333	enabling the production of V1/V2 scaffolds in a cell line suitable for biopharmaceutical production	2326:2424	The scaffolds produced address three major problems in HIV vaccine development: (1) improving antibody responses to poorly immunogenic epitopes in the V1/V2 domain; (2) eliminating antibody responses to highly immunogenic (decoy) epitopes outside the V1/V2 domain; and (3) enabling the production of V1/V2 scaffolds in a cell line suitable for biopharmaceutical production.
27449907	9	80	dep	produced	2067:2074	arg1	improving					2137:2145	improving	2137:2145	improving antibody responses to poorly immunogenic epitopes in the V1/V2 domain; (2) eliminating antibody responses to highly immunogenic (decoy) epitopes outside the V1/V2 domain	2137:2315	The scaffolds produced address three major problems in HIV vaccine development: (1) improving antibody responses to poorly immunogenic epitopes in the V1/V2 domain; (2) eliminating antibody responses to highly immunogenic (decoy) epitopes outside the V1/V2 domain; and (3) enabling the production of V1/V2 scaffolds in a cell line suitable for biopharmaceutical production.
27449907	9	80	dep	produced	2067:2074	arg1	1					2134:2134	1	2134:2134	1	2134:2134	The scaffolds produced address three major problems in HIV vaccine development: (1) improving antibody responses to poorly immunogenic epitopes in the V1/V2 domain; (2) eliminating antibody responses to highly immunogenic (decoy) epitopes outside the V1/V2 domain; and (3) enabling the production of V1/V2 scaffolds in a cell line suitable for biopharmaceutical production.
27449907	3	81	from	advantageous	895:906	arg1	vaccine					925:931	a multivalent vaccine	911:931	a multivalent vaccine	911:931	Previous studies showed that V1/V2 domain scaffolds could be produced from a few selected isolates, but not from many of the isolates that would be advantageous in a multivalent vaccine.
27449907	2	82	theme	potential	674:682	arg1	way					684:686	a potential way	672:686	a potential way to enhance the immune response to these specific epitopes	672:744	Because both of these epitopes are poorly immunogenic in the context of full length envelope proteins, immunization with properly folded and glycosylated fragments (scaffolds) represents a potential way to enhance the immune response to these specific epitopes.
27449907	3	83	theme	Previous	747:754	arg1	studies					756:762	Previous studies	747:762	Previous studies	747:762	Previous studies showed that V1/V2 domain scaffolds could be produced from a few selected isolates, but not from many of the isolates that would be advantageous in a multivalent vaccine.
27449907	8	84	theme	PG9	1879:1881	arg1	binding					1883:1889	PG9 binding	1879:1889	PG9 binding to scaffolds produced in normal cells	1879:1927	The mutations that improved PG9 binding to scaffolds produced in normal cells included amino acid positions outside of the antibody contact region designed to stabilize the β-sheet and turn structures.
27449907	6	85	theme	biopharmaceutical	1566:1582	arg1	production					1584:1593	biopharmaceutical production	1566:1593	biopharmaceutical production of proteins intended for clinical trials	1566:1634	Although scaffolds incorporating the glycans required for PG9 binding (e.g., mannose-5) can be produced using glycosylation inhibitors (e.g., swainsonine), or mutant cell lines (e.g. GnTI(-) 293 HEK), these are not practical for biopharmaceutical production of proteins intended for clinical trials.
27449907	5	86	theme	correct	1253:1259	arg1	structure					1268:1276	the correct glycan structure	1249:1276	the correct glycan structure	1249:1276	Significantly, this effort required replicating both the correct glycan structure as well as the β-sheet structure required for PG9 binding.
27449907	4	87	from	stability	1018:1026	arg1	several					1126:1132	several	1126:1132	several	1126:1132	In this paper, we used a protein engineering approach to improve the conformational stability and antibody binding activity of V1/V2 domain scaffolds from multiple diverse isolates, including several that were initially unable to bind the prototypic PG9 bN-mAb.
27449907	4	87	from	stability	1018:1026	arg1	isolates					1106:1113	multiple diverse isolates	1089:1113	multiple diverse isolates	1089:1113	In this paper, we used a protein engineering approach to improve the conformational stability and antibody binding activity of V1/V2 domain scaffolds from multiple diverse isolates, including several that were initially unable to bind the prototypic PG9 bN-mAb.
27449907	3	88	theme	few	824:826	arg1	isolates					837:844	a few selected isolates	822:844	a few selected isolates	822:844	Previous studies showed that V1/V2 domain scaffolds could be produced from a few selected isolates, but not from many of the isolates that would be advantageous in a multivalent vaccine.
27449907	7	89	gly	glycopeptide	1676:1687	arg2	glycopeptide					1676:1687	engineered glycopeptide scaffolds	1665:1697	engineered glycopeptide scaffolds from three different clades of HIV-1 that bind PG9 with high affinity when expressed in a wildtype cell line suitable for biopharmaceutical production	1665:1848	In this report, we describe engineered glycopeptide scaffolds from three different clades of HIV-1 that bind PG9 with high affinity when expressed in a wildtype cell line suitable for biopharmaceutical production.
27449907	3	90	theme	V1/V2	776:780	arg1	scaffolds					789:797	V1/V2 domain scaffolds	776:797	V1/V2 domain scaffolds	776:797	Previous studies showed that V1/V2 domain scaffolds could be produced from a few selected isolates, but not from many of the isolates that would be advantageous in a multivalent vaccine.
27449907	0	91	theme	improved	71:78	arg1	binding					80:86	improved binding	71:86	improved binding to the broadly neutralizing PG9 antibody	71:127	Fragments of the V1/V2 domain of HIV-1 glycoprotein 120 engineered for improved binding to the broadly neutralizing PG9 antibody.
27449907	9	92	theme	scaffolds	2359:2367	arg1	production					2339:2348	the production	2335:2348	the production of V1/V2 scaffolds in a cell line suitable for biopharmaceutical production	2335:2424	The scaffolds produced address three major problems in HIV vaccine development: (1) improving antibody responses to poorly immunogenic epitopes in the V1/V2 domain; (2) eliminating antibody responses to highly immunogenic (decoy) epitopes outside the V1/V2 domain; and (3) enabling the production of V1/V2 scaffolds in a cell line suitable for biopharmaceutical production.
27449907	6	93	theme	cell	1503:1506	arg1	lines					1508:1512	mutant cell lines	1496:1512	mutant cell lines (e.g. GnTI(-) 293 HEK)	1496:1535	Although scaffolds incorporating the glycans required for PG9 binding (e.g., mannose-5) can be produced using glycosylation inhibitors (e.g., swainsonine), or mutant cell lines (e.g. GnTI(-) 293 HEK), these are not practical for biopharmaceutical production of proteins intended for clinical trials.
27449907	6	93	theme	cell	1503:1506	arg1	HEK					1532:1534	293 HEK	1528:1534	293 HEK	1528:1534	Although scaffolds incorporating the glycans required for PG9 binding (e.g., mannose-5) can be produced using glycosylation inhibitors (e.g., swainsonine), or mutant cell lines (e.g. GnTI(-) 293 HEK), these are not practical for biopharmaceutical production of proteins intended for clinical trials.
27449907	6	94	dep	GnTI	1520:1523	arg1	e.g.					1515:1518	e.g.	1515:1518	e.g.	1515:1518	Although scaffolds incorporating the glycans required for PG9 binding (e.g., mannose-5) can be produced using glycosylation inhibitors (e.g., swainsonine), or mutant cell lines (e.g. GnTI(-) 293 HEK), these are not practical for biopharmaceutical production of proteins intended for clinical trials.
27449907	8	95	theme	normal	1916:1921	arg1	cells					1923:1927	normal cells	1916:1927	normal cells	1916:1927	The mutations that improved PG9 binding to scaffolds produced in normal cells included amino acid positions outside of the antibody contact region designed to stabilize the β-sheet and turn structures.
27449907	0	96	theme	broadly	95:101	arg1	antibody					120:127	the broadly neutralizing PG9 antibody	91:127	the broadly neutralizing PG9 antibody	91:127	Fragments of the V1/V2 domain of HIV-1 glycoprotein 120 engineered for improved binding to the broadly neutralizing PG9 antibody.
27449907	3	97	theme	isolates	872:879	arg1	many					860:863	many	860:863	many	860:863	Previous studies showed that V1/V2 domain scaffolds could be produced from a few selected isolates, but not from many of the isolates that would be advantageous in a multivalent vaccine.
27449907	3	97	theme	isolates	872:879	arg1	isolates					872:879	the isolates	868:879	the isolates that would be advantageous in a multivalent vaccine	868:931	Previous studies showed that V1/V2 domain scaffolds could be produced from a few selected isolates, but not from many of the isolates that would be advantageous in a multivalent vaccine.
27449907	3	97	theme	isolates	872:879	arg1	advantageous					895:906	advantageous	895:906	advantageous	895:906	Previous studies showed that V1/V2 domain scaffolds could be produced from a few selected isolates, but not from many of the isolates that would be advantageous in a multivalent vaccine.
27449907	8	98	theme	acid	1944:1947	arg1	positions					1949:1957	amino acid positions	1938:1957	amino acid positions outside of the antibody contact region designed to stabilize the β-sheet and turn structures	1938:2050	The mutations that improved PG9 binding to scaffolds produced in normal cells included amino acid positions outside of the antibody contact region designed to stabilize the β-sheet and turn structures.
27449907	7	99	theme	biopharmaceutical	1821:1837	arg1	production					1839:1848	biopharmaceutical production	1821:1848	biopharmaceutical production	1821:1848	In this report, we describe engineered glycopeptide scaffolds from three different clades of HIV-1 that bind PG9 with high affinity when expressed in a wildtype cell line suitable for biopharmaceutical production.
27449907	4	100	theme	protein	959:965	arg1	approach					979:986	a protein engineering approach	957:986	a protein engineering approach	957:986	In this paper, we used a protein engineering approach to improve the conformational stability and antibody binding activity of V1/V2 domain scaffolds from multiple diverse isolates, including several that were initially unable to bind the prototypic PG9 bN-mAb.
27449907	4	101	theme	PG9	1184:1186	arg1	bN-mAb					1188:1193	the prototypic PG9 bN-mAb	1169:1193	the prototypic PG9 bN-mAb	1169:1193	In this paper, we used a protein engineering approach to improve the conformational stability and antibody binding activity of V1/V2 domain scaffolds from multiple diverse isolates, including several that were initially unable to bind the prototypic PG9 bN-mAb.
27449907	0	102	theme	V1/V2	17:21	arg1	domain					23:28	the V1/V2 domain	13:28	the V1/V2 domain of HIV-1 glycoprotein 120	13:54	Fragments of the V1/V2 domain of HIV-1 glycoprotein 120 engineered for improved binding to the broadly neutralizing PG9 antibody.
27449907	0	102	theme	V1/V2	17:21	arg1	glycoprotein					39:50	HIV-1 glycoprotein 120	33:54	HIV-1 glycoprotein 120	33:54	Fragments of the V1/V2 domain of HIV-1 glycoprotein 120 engineered for improved binding to the broadly neutralizing PG9 antibody.
27449907	1	103	theme	broadly	273:279	arg1	bN-mAb					315:320	bN-mAb	315:320	bN-mAb	315:320	The V1/V2 domain of the HIV-1 envelope protein gp120 possesses two important epitopes: a glycan-dependent epitope recognized by the prototypic broadly neutralizing monoclonal antibody (bN-mAb), PG9, as well as an epitope recognized by non-neutralizing antibodies that has been associated with protection from HIV infection in the RV144 HIV vaccine trial.
27449907	1	103	theme	broadly	273:279	arg1	antibody					305:312	the prototypic broadly neutralizing monoclonal antibody	258:312	the prototypic broadly neutralizing monoclonal antibody (bN-mAb)	258:321	The V1/V2 domain of the HIV-1 envelope protein gp120 possesses two important epitopes: a glycan-dependent epitope recognized by the prototypic broadly neutralizing monoclonal antibody (bN-mAb), PG9, as well as an epitope recognized by non-neutralizing antibodies that has been associated with protection from HIV infection in the RV144 HIV vaccine trial.
27449907	7	104	theme	suitable	1808:1815	arg1	line					1803:1806	a wildtype cell line	1787:1806	a wildtype cell line suitable for biopharmaceutical production	1787:1848	In this report, we describe engineered glycopeptide scaffolds from three different clades of HIV-1 that bind PG9 with high affinity when expressed in a wildtype cell line suitable for biopharmaceutical production.
27449907	8	105	theme	β-sheet	2024:2030	arg1	structures					2041:2050	the β-sheet and turn structures	2020:2050	structures	2041:2050	The mutations that improved PG9 binding to scaffolds produced in normal cells included amino acid positions outside of the antibody contact region designed to stabilize the β-sheet and turn structures.
27449907	6	106	theme	PG9	1395:1397	arg1	binding					1399:1405	PG9 binding	1395:1405	PG9 binding	1395:1405	Although scaffolds incorporating the glycans required for PG9 binding (e.g., mannose-5) can be produced using glycosylation inhibitors (e.g., swainsonine), or mutant cell lines (e.g. GnTI(-) 293 HEK), these are not practical for biopharmaceutical production of proteins intended for clinical trials.
27449907	1	107	theme	monoclonal	294:303	arg1	bN-mAb					315:320	bN-mAb	315:320	bN-mAb	315:320	The V1/V2 domain of the HIV-1 envelope protein gp120 possesses two important epitopes: a glycan-dependent epitope recognized by the prototypic broadly neutralizing monoclonal antibody (bN-mAb), PG9, as well as an epitope recognized by non-neutralizing antibodies that has been associated with protection from HIV infection in the RV144 HIV vaccine trial.
27449907	1	107	theme	monoclonal	294:303	arg1	antibody					305:312	the prototypic broadly neutralizing monoclonal antibody	258:312	the prototypic broadly neutralizing monoclonal antibody (bN-mAb)	258:321	The V1/V2 domain of the HIV-1 envelope protein gp120 possesses two important epitopes: a glycan-dependent epitope recognized by the prototypic broadly neutralizing monoclonal antibody (bN-mAb), PG9, as well as an epitope recognized by non-neutralizing antibodies that has been associated with protection from HIV infection in the RV144 HIV vaccine trial.
27449907	7	108	theme	cell	1798:1801	arg1	line					1803:1806	a wildtype cell line	1787:1806	a wildtype cell line suitable for biopharmaceutical production	1787:1848	In this report, we describe engineered glycopeptide scaffolds from three different clades of HIV-1 that bind PG9 with high affinity when expressed in a wildtype cell line suitable for biopharmaceutical production.
27449907	9	109	dep	responses	2156:2164	arg1	responses					2243:2251	antibody responses	2234:2251	antibody responses to poorly immunogenic epitopes in the V1/V2 domain; (2) eliminating antibody responses to highly immunogenic (decoy) epitopes outside the V1/V2 domain	2147:2315	The scaffolds produced address three major problems in HIV vaccine development: (1) improving antibody responses to poorly immunogenic epitopes in the V1/V2 domain; (2) eliminating antibody responses to highly immunogenic (decoy) epitopes outside the V1/V2 domain; and (3) enabling the production of V1/V2 scaffolds in a cell line suitable for biopharmaceutical production.
27449907	8	110	theme	turn	2036:2039	arg1	structures					2041:2050	the β-sheet and turn structures	2020:2050	structures	2041:2050	The mutations that improved PG9 binding to scaffolds produced in normal cells included amino acid positions outside of the antibody contact region designed to stabilize the β-sheet and turn structures.
27449907	1	111	theme	HIV	466:468	arg1	trial					478:482	the RV144 HIV vaccine trial	456:482	the RV144 HIV vaccine trial	456:482	The V1/V2 domain of the HIV-1 envelope protein gp120 possesses two important epitopes: a glycan-dependent epitope recognized by the prototypic broadly neutralizing monoclonal antibody (bN-mAb), PG9, as well as an epitope recognized by non-neutralizing antibodies that has been associated with protection from HIV infection in the RV144 HIV vaccine trial.
27449907	0	112	theme	glycoprotein	39:50	arg1	domain					23:28	the V1/V2 domain	13:28	the V1/V2 domain of HIV-1 glycoprotein 120	13:54	Fragments of the V1/V2 domain of HIV-1 glycoprotein 120 engineered for improved binding to the broadly neutralizing PG9 antibody.
27449907	0	112	theme	glycoprotein	39:50	arg1	glycoprotein					39:50	HIV-1 glycoprotein 120	33:54	HIV-1 glycoprotein 120	33:54	Fragments of the V1/V2 domain of HIV-1 glycoprotein 120 engineered for improved binding to the broadly neutralizing PG9 antibody.
27449907	6	113	dep	mannose-5	1414:1422	arg1	e.g.					1408:1411	e.g.	1408:1411	e.g.	1408:1411	Although scaffolds incorporating the glycans required for PG9 binding (e.g., mannose-5) can be produced using glycosylation inhibitors (e.g., swainsonine), or mutant cell lines (e.g. GnTI(-) 293 HEK), these are not practical for biopharmaceutical production of proteins intended for clinical trials.
27449907	9	114	theme	antibody	2234:2241	arg1	responses					2243:2251	antibody responses	2234:2251	antibody responses to poorly immunogenic epitopes in the V1/V2 domain; (2) eliminating antibody responses to highly immunogenic (decoy) epitopes outside the V1/V2 domain	2147:2315	The scaffolds produced address three major problems in HIV vaccine development: (1) improving antibody responses to poorly immunogenic epitopes in the V1/V2 domain; (2) eliminating antibody responses to highly immunogenic (decoy) epitopes outside the V1/V2 domain; and (3) enabling the production of V1/V2 scaffolds in a cell line suitable for biopharmaceutical production.
27449907	7	115	theme	high	1755:1758	arg1	affinity					1760:1767	high affinity	1755:1767	high affinity	1755:1767	In this report, we describe engineered glycopeptide scaffolds from three different clades of HIV-1 that bind PG9 with high affinity when expressed in a wildtype cell line suitable for biopharmaceutical production.
27449907	1	116	from	infection	443:451	arg1	trial					478:482	the RV144 HIV vaccine trial	456:482	the RV144 HIV vaccine trial	456:482	The V1/V2 domain of the HIV-1 envelope protein gp120 possesses two important epitopes: a glycan-dependent epitope recognized by the prototypic broadly neutralizing monoclonal antibody (bN-mAb), PG9, as well as an epitope recognized by non-neutralizing antibodies that has been associated with protection from HIV infection in the RV144 HIV vaccine trial.
27449907	1	116	from	infection	443:451	arg1	protection					423:432	protection	423:432	protection from HIV infection in the RV144 HIV vaccine trial	423:482	The V1/V2 domain of the HIV-1 envelope protein gp120 possesses two important epitopes: a glycan-dependent epitope recognized by the prototypic broadly neutralizing monoclonal antibody (bN-mAb), PG9, as well as an epitope recognized by non-neutralizing antibodies that has been associated with protection from HIV infection in the RV144 HIV vaccine trial.
27449907	5	117	dep	structure	1268:1276	arg1	both					1244:1247	both	1244:1247	both	1244:1247	Significantly, this effort required replicating both the correct glycan structure as well as the β-sheet structure required for PG9 binding.
27449907	1	118	contain	possesses	183:191	arg2	epitope					236:242	a glycan-dependent epitope	217:242	a glycan-dependent epitope recognized by the prototypic broadly neutralizing monoclonal antibody (bN-mAb)	217:321	The V1/V2 domain of the HIV-1 envelope protein gp120 possesses two important epitopes: a glycan-dependent epitope recognized by the prototypic broadly neutralizing monoclonal antibody (bN-mAb), PG9, as well as an epitope recognized by non-neutralizing antibodies that has been associated with protection from HIV infection in the RV144 HIV vaccine trial.
27449907	1	118	contain	possesses	183:191	arg2	PG9					324:326	PG9	324:326	PG9	324:326	The V1/V2 domain of the HIV-1 envelope protein gp120 possesses two important epitopes: a glycan-dependent epitope recognized by the prototypic broadly neutralizing monoclonal antibody (bN-mAb), PG9, as well as an epitope recognized by non-neutralizing antibodies that has been associated with protection from HIV infection in the RV144 HIV vaccine trial.
27449907	1	118	contain	possesses	183:191	arg1	domain					140:145	The V1/V2 domain	130:145	The V1/V2 domain of the HIV-1 envelope protein gp120	130:181	The V1/V2 domain of the HIV-1 envelope protein gp120 possesses two important epitopes: a glycan-dependent epitope recognized by the prototypic broadly neutralizing monoclonal antibody (bN-mAb), PG9, as well as an epitope recognized by non-neutralizing antibodies that has been associated with protection from HIV infection in the RV144 HIV vaccine trial.
27449907	1	118	contain	possesses	183:191	arg1	gp120					177:181	the HIV-1 envelope protein gp120	150:181	the HIV-1 envelope protein gp120	150:181	The V1/V2 domain of the HIV-1 envelope protein gp120 possesses two important epitopes: a glycan-dependent epitope recognized by the prototypic broadly neutralizing monoclonal antibody (bN-mAb), PG9, as well as an epitope recognized by non-neutralizing antibodies that has been associated with protection from HIV infection in the RV144 HIV vaccine trial.
27449907	1	118	contain	possesses	183:191	arg2	epitope					343:349	an epitope	340:349	an epitope recognized by non-neutralizing antibodies that has been associated with protection from HIV infection in the RV144 HIV vaccine trial	340:482	The V1/V2 domain of the HIV-1 envelope protein gp120 possesses two important epitopes: a glycan-dependent epitope recognized by the prototypic broadly neutralizing monoclonal antibody (bN-mAb), PG9, as well as an epitope recognized by non-neutralizing antibodies that has been associated with protection from HIV infection in the RV144 HIV vaccine trial.
27449907	1	118	contain	possesses	183:191	arg2	epitopes					207:214	two important epitopes	193:214	two important epitopes: a glycan-dependent epitope recognized by the prototypic broadly neutralizing monoclonal antibody (bN-mAb), PG9, as well as an epitope recognized by non-neutralizing antibodies that has been associated with protection from HIV infection in the RV144 HIV vaccine trial	193:482	The V1/V2 domain of the HIV-1 envelope protein gp120 possesses two important epitopes: a glycan-dependent epitope recognized by the prototypic broadly neutralizing monoclonal antibody (bN-mAb), PG9, as well as an epitope recognized by non-neutralizing antibodies that has been associated with protection from HIV infection in the RV144 HIV vaccine trial.
27449907	4	119	theme	binding	1041:1047	arg1	activity					1049:1056	antibody binding activity	1032:1056	antibody binding activity	1032:1056	In this paper, we used a protein engineering approach to improve the conformational stability and antibody binding activity of V1/V2 domain scaffolds from multiple diverse isolates, including several that were initially unable to bind the prototypic PG9 bN-mAb.
27449907	2	120	from	immunogenic	527:537	arg1	context					546:552	the context	542:552	the context of full length envelope proteins	542:585	Because both of these epitopes are poorly immunogenic in the context of full length envelope proteins, immunization with properly folded and glycosylated fragments (scaffolds) represents a potential way to enhance the immune response to these specific epitopes.
27449907	9	121	theme	immunogenic	2263:2273	arg1	epitopes					2283:2290	highly immunogenic (decoy) epitopes	2256:2290	highly immunogenic (decoy) epitopes outside the V1/V2 domain	2256:2315	The scaffolds produced address three major problems in HIV vaccine development: (1) improving antibody responses to poorly immunogenic epitopes in the V1/V2 domain; (2) eliminating antibody responses to highly immunogenic (decoy) epitopes outside the V1/V2 domain; and (3) enabling the production of V1/V2 scaffolds in a cell line suitable for biopharmaceutical production.
27449907	1	122	theme	glycan-dependent	219:234	arg1	epitopes					207:214	two important epitopes	193:214	two important epitopes: a glycan-dependent epitope recognized by the prototypic broadly neutralizing monoclonal antibody (bN-mAb), PG9, as well as an epitope recognized by non-neutralizing antibodies that has been associated with protection from HIV infection in the RV144 HIV vaccine trial	193:482	The V1/V2 domain of the HIV-1 envelope protein gp120 possesses two important epitopes: a glycan-dependent epitope recognized by the prototypic broadly neutralizing monoclonal antibody (bN-mAb), PG9, as well as an epitope recognized by non-neutralizing antibodies that has been associated with protection from HIV infection in the RV144 HIV vaccine trial.
27449907	1	122	theme	glycan-dependent	219:234	arg1	epitope					236:242	a glycan-dependent epitope	217:242	a glycan-dependent epitope recognized by the prototypic broadly neutralizing monoclonal antibody (bN-mAb)	217:321	The V1/V2 domain of the HIV-1 envelope protein gp120 possesses two important epitopes: a glycan-dependent epitope recognized by the prototypic broadly neutralizing monoclonal antibody (bN-mAb), PG9, as well as an epitope recognized by non-neutralizing antibodies that has been associated with protection from HIV infection in the RV144 HIV vaccine trial.
27236494	2	0	theme	size	468:471	arg1	>400 μm					473:479	size >400 μm	468:479	size >400 μm	468:479	It was shown that the presence of high concentrations of microplastics with size >400 μm did not directly impact the growth of microalgae in the first days of contact and that the expression of three genes involved in the stress response was not modified after 78 days.
27236494	4	1	theme	PP	783:784	arg1	case					775:778	the case	771:778	the case of PP only	771:789	However, after 20 days of contact, in the case of PP only, hetero-aggregates constituted of microalgae, microplastics and exopolysaccharides were formed.
27236494	1	2	theme	Chlamydomas	348:358	arg1	microalgae					336:345	a model freshwater microalgae	317:345	a model freshwater microalgae	317:345	In this study, the interactions between microplastics, chosen among the most widely used in industry such as polypropylene (PP) and high-density polyethylene (HDPE), and a model freshwater microalgae, Chlamydomas reinhardtii, were investigated.
27236494	1	2	theme	Chlamydomas	348:358	arg1	reinhardtii					360:370	Chlamydomas reinhardtii	348:370	Chlamydomas reinhardtii	348:370	In this study, the interactions between microplastics, chosen among the most widely used in industry such as polypropylene (PP) and high-density polyethylene (HDPE), and a model freshwater microalgae, Chlamydomas reinhardtii, were investigated.
27236494	6	3	from	surface	1158:1164	arg1	transport					1113:1121	the vertical transport	1100:1121	the vertical transport of PP microplastics from the water surface to sediment	1100:1176	Such hetero-aggregates appear as an important pathway for the vertical transport of PP microplastics from the water surface to sediment.
27236494	2	4	theme	microalgae	519:528	arg1	growth					509:514	the growth	505:514	the growth of microalgae	505:528	It was shown that the presence of high concentrations of microplastics with size >400 μm did not directly impact the growth of microalgae in the first days of contact and that the expression of three genes involved in the stress response was not modified after 78 days.
27236494	0	5	from	interactions	13:24	arg1	density					96:102	plastic density	88:102	plastic density	88:102	Microplastic interactions with freshwater microalgae: Hetero-aggregation and changes in plastic density appear strongly dependent on polymer type.
27236494	2	6	theme	stress	614:619	arg1	response					621:628	the stress response	610:628	the stress response	610:628	It was shown that the presence of high concentrations of microplastics with size >400 μm did not directly impact the growth of microalgae in the first days of contact and that the expression of three genes involved in the stress response was not modified after 78 days.
27236494	1	7	theme	model	319:323	arg1	microalgae					336:345	a model freshwater microalgae	317:345	a model freshwater microalgae	317:345	In this study, the interactions between microplastics, chosen among the most widely used in industry such as polypropylene (PP) and high-density polyethylene (HDPE), and a model freshwater microalgae, Chlamydomas reinhardtii, were investigated.
27236494	1	7	theme	model	319:323	arg1	reinhardtii					360:370	Chlamydomas reinhardtii	348:370	Chlamydomas reinhardtii	348:370	In this study, the interactions between microplastics, chosen among the most widely used in industry such as polypropylene (PP) and high-density polyethylene (HDPE), and a model freshwater microalgae, Chlamydomas reinhardtii, were investigated.
27236494	9	8	theme	different	1559:1567	arg1	fates					1569:1573	different fates	1559:1573	different fates in the environment	1559:1592	This work presents the first evidence that depending on their chemical nature, microplastics will follow different fates in the environment.
27236494	5	9	theme	PP	963:964	arg1	fragments					966:974	PP fragments	963:974	PP fragments	963:974	An estimation of the hetero-aggregates composition was approximately 50% of PP fragments and 50% of microalgae, which led to a final density close to 1.2.
27236494	1	10	theme	freshwater	325:334	arg1	microalgae					336:345	a model freshwater microalgae	317:345	a model freshwater microalgae	317:345	In this study, the interactions between microplastics, chosen among the most widely used in industry such as polypropylene (PP) and high-density polyethylene (HDPE), and a model freshwater microalgae, Chlamydomas reinhardtii, were investigated.
27236494	1	10	theme	freshwater	325:334	arg1	reinhardtii					360:370	Chlamydomas reinhardtii	348:370	Chlamydomas reinhardtii	348:370	In this study, the interactions between microplastics, chosen among the most widely used in industry such as polypropylene (PP) and high-density polyethylene (HDPE), and a model freshwater microalgae, Chlamydomas reinhardtii, were investigated.
27236494	2	11	theme	contact	551:557	arg1	days					543:546	the first days	533:546	the first days of contact	533:557	It was shown that the presence of high concentrations of microplastics with size >400 μm did not directly impact the growth of microalgae in the first days of contact and that the expression of three genes involved in the stress response was not modified after 78 days.
27236494	7	12	theme	control	1350:1356	arg1	conditions					1358:1367	control conditions	1350:1367	control conditions	1350:1367	Moreover, after more than 70 days of contact with microplastics, the microalgae genes involved in the sugar biosynthesis pathways were strongly over-expressed compared to control conditions.
27236494	8	13	theme	HDPE	1427:1430	arg1	case					1419:1422	the case	1415:1422	the case of HDPE	1415:1430	The levels of over-expression were higher in the case of HDPE than in PP condition.
27236494	2	14	theme	genes	592:596	arg1	expression					572:581	the expression	568:581	the expression of three genes involved in the stress response	568:628	It was shown that the presence of high concentrations of microplastics with size >400 μm did not directly impact the growth of microalgae in the first days of contact and that the expression of three genes involved in the stress response was not modified after 78 days.
27236494	0	15	with	changes	77:83	arg1	Hetero-aggregation					54:71	freshwater microalgae: Hetero-aggregation	31:71	freshwater microalgae: Hetero-aggregation	31:71	Microplastic interactions with freshwater microalgae: Hetero-aggregation and changes in plastic density appear strongly dependent on polymer type.
27236494	6	16	theme	important	1078:1086	arg1	pathway					1088:1094	an important pathway	1075:1094	an important pathway for the vertical transport of PP microplastics from the water surface to sediment	1075:1176	Such hetero-aggregates appear as an important pathway for the vertical transport of PP microplastics from the water surface to sediment.
27236494	5	17	theme	final	1014:1018	arg1	density					1020:1026	a final density	1012:1026	a final density close to 1.2	1012:1039	An estimation of the hetero-aggregates composition was approximately 50% of PP fragments and 50% of microalgae, which led to a final density close to 1.2.
27236494	5	18	theme	hetero-aggregates	908:924	arg1	composition					926:936	the hetero-aggregates composition	904:936	the hetero-aggregates composition	904:936	An estimation of the hetero-aggregates composition was approximately 50% of PP fragments and 50% of microalgae, which led to a final density close to 1.2.
27236494	0	19	theme	plastic	88:94	arg1	density					96:102	plastic density	88:102	plastic density	88:102	Microplastic interactions with freshwater microalgae: Hetero-aggregation and changes in plastic density appear strongly dependent on polymer type.
27236494	0	20	theme	Microplastic	0:11	arg1	interactions					13:24	Microplastic interactions	0:24	Microplastic interactions with freshwater microalgae: Hetero-aggregation	0:71	Microplastic interactions with freshwater microalgae: Hetero-aggregation and changes in plastic density appear strongly dependent on polymer type.
27236494	6	21	theme	water	1152:1156	arg1	surface					1158:1164	the water surface	1148:1164	the water surface	1148:1164	Such hetero-aggregates appear as an important pathway for the vertical transport of PP microplastics from the water surface to sediment.
27236494	7	22	theme	contact	1216:1222	arg1	days					1208:1211	more than 70 days	1195:1211	more than 70 days of contact with microplastics	1195:1241	Moreover, after more than 70 days of contact with microplastics, the microalgae genes involved in the sugar biosynthesis pathways were strongly over-expressed compared to control conditions.
27236494	0	23	theme	freshwater	31:40	arg1	Hetero-aggregation					54:71	freshwater microalgae: Hetero-aggregation	31:71	freshwater microalgae: Hetero-aggregation	31:71	Microplastic interactions with freshwater microalgae: Hetero-aggregation and changes in plastic density appear strongly dependent on polymer type.
27236494	6	24	theme	PP	1126:1127	arg1	microplastics					1129:1141	PP microplastics	1126:1141	PP microplastics	1126:1141	Such hetero-aggregates appear as an important pathway for the vertical transport of PP microplastics from the water surface to sediment.
27236494	7	25	theme	biosynthesis	1287:1298	arg1	pathways					1300:1307	the sugar biosynthesis pathways	1277:1307	the sugar biosynthesis pathways	1277:1307	Moreover, after more than 70 days of contact with microplastics, the microalgae genes involved in the sugar biosynthesis pathways were strongly over-expressed compared to control conditions.
27236494	9	26	theme	chemical	1516:1523	arg1	nature					1525:1530	their chemical nature	1510:1530	their chemical nature	1510:1530	This work presents the first evidence that depending on their chemical nature, microplastics will follow different fates in the environment.
27236494	3	27	theme	similar	677:683	arg1	colonization					685:696	a similar colonization	675:696	a similar colonization	675:696	In parallel, a similar colonization was observed for the two polymers.
27236494	0	28	theme	polymer	133:139	arg1	type					141:144	polymer type	133:144	polymer type	133:144	Microplastic interactions with freshwater microalgae: Hetero-aggregation and changes in plastic density appear strongly dependent on polymer type.
27236494	5	29	theme	microalgae	987:996	arg1	%					982:982	50%	980:982	50% of microalgae, which led to a final density close to 1.2	980:1039	An estimation of the hetero-aggregates composition was approximately 50% of PP fragments and 50% of microalgae, which led to a final density close to 1.2.
27236494	5	29	theme	microalgae	987:996	arg1	estimation					890:899	An estimation	887:899	An estimation of the hetero-aggregates composition	887:936	An estimation of the hetero-aggregates composition was approximately 50% of PP fragments and 50% of microalgae, which led to a final density close to 1.2.
27236494	5	29	theme	microalgae	987:996	arg1	microalgae					987:996	microalgae	987:996	microalgae	987:996	An estimation of the hetero-aggregates composition was approximately 50% of PP fragments and 50% of microalgae, which led to a final density close to 1.2.
27236494	5	29	theme	microalgae	987:996	arg1	%					958:958	approximately 50%	942:958	approximately 50% of PP fragments	942:974	An estimation of the hetero-aggregates composition was approximately 50% of PP fragments and 50% of microalgae, which led to a final density close to 1.2.
27236494	5	29	theme	microalgae	987:996	arg1	fragments					966:974	PP fragments	963:974	PP fragments	963:974	An estimation of the hetero-aggregates composition was approximately 50% of PP fragments and 50% of microalgae, which led to a final density close to 1.2.
27236494	6	30	theme	Such	1042:1045	arg1	hetero-aggregates					1047:1063	Such hetero-aggregates	1042:1063	Such hetero-aggregates	1042:1063	Such hetero-aggregates appear as an important pathway for the vertical transport of PP microplastics from the water surface to sediment.
27236494	2	31	theme	microplastics	449:461	arg1	concentrations					431:444	high concentrations	426:444	high concentrations of microplastics with size >400 μm	426:479	It was shown that the presence of high concentrations of microplastics with size >400 μm did not directly impact the growth of microalgae in the first days of contact and that the expression of three genes involved in the stress response was not modified after 78 days.
27236494	1	32	theme	high-density	279:290	arg1	HDPE					306:309	HDPE	306:309	HDPE	306:309	In this study, the interactions between microplastics, chosen among the most widely used in industry such as polypropylene (PP) and high-density polyethylene (HDPE), and a model freshwater microalgae, Chlamydomas reinhardtii, were investigated.
27236494	1	32	theme	high-density	279:290	arg1	polyethylene					292:303	high-density polyethylene	279:303	high-density polyethylene (HDPE)	279:310	In this study, the interactions between microplastics, chosen among the most widely used in industry such as polypropylene (PP) and high-density polyethylene (HDPE), and a model freshwater microalgae, Chlamydomas reinhardtii, were investigated.
27236494	6	33	theme	microplastics	1129:1141	arg1	transport					1113:1121	the vertical transport	1100:1121	the vertical transport of PP microplastics from the water surface to sediment	1100:1176	Such hetero-aggregates appear as an important pathway for the vertical transport of PP microplastics from the water surface to sediment.
27236494	0	34	theme	microalgae	42:51	arg1	Hetero-aggregation					54:71	freshwater microalgae: Hetero-aggregation	31:71	freshwater microalgae: Hetero-aggregation	31:71	Microplastic interactions with freshwater microalgae: Hetero-aggregation and changes in plastic density appear strongly dependent on polymer type.
27236494	7	35	theme	sugar	1281:1285	arg1	pathways					1300:1307	the sugar biosynthesis pathways	1277:1307	the sugar biosynthesis pathways	1277:1307	Moreover, after more than 70 days of contact with microplastics, the microalgae genes involved in the sugar biosynthesis pathways were strongly over-expressed compared to control conditions.
27236494	2	36	theme	concentrations	431:444	arg1	presence					414:421	the presence	410:421	the presence of high concentrations of microplastics with size >400 μm	410:479	It was shown that the presence of high concentrations of microplastics with size >400 μm did not directly impact the growth of microalgae in the first days of contact and that the expression of three genes involved in the stress response was not modified after 78 days.
27236494	9	37	from	fates	1569:1573	arg1	environment					1582:1592	the environment	1578:1592	the environment	1578:1592	This work presents the first evidence that depending on their chemical nature, microplastics will follow different fates in the environment.
27236494	3	38	located	observed	702:709	arg1	parallel					665:672	parallel	665:672	parallel	665:672	In parallel, a similar colonization was observed for the two polymers.
27236494	3	38	located	observed	702:709	arg2	colonization					685:696	a similar colonization	675:696	a similar colonization	675:696	In parallel, a similar colonization was observed for the two polymers.
27236494	0	39	from	changes	77:83	arg1	density					96:102	plastic density	88:102	plastic density	88:102	Microplastic interactions with freshwater microalgae: Hetero-aggregation and changes in plastic density appear strongly dependent on polymer type.
27236494	4	40	theme	contact	759:765	arg1	days					751:754	20 days	748:754	20 days of contact	748:765	However, after 20 days of contact, in the case of PP only, hetero-aggregates constituted of microalgae, microplastics and exopolysaccharides were formed.
27236494	8	41	theme	PP	1440:1441	arg1	condition					1443:1451	PP condition	1440:1451	PP condition	1440:1451	The levels of over-expression were higher in the case of HDPE than in PP condition.
27236494	2	42	theme	high	426:429	arg1	concentrations					431:444	high concentrations	426:444	high concentrations of microplastics with size >400 μm	426:479	It was shown that the presence of high concentrations of microplastics with size >400 μm did not directly impact the growth of microalgae in the first days of contact and that the expression of three genes involved in the stress response was not modified after 78 days.
27236494	7	43	with	contact	1216:1222	arg1	microplastics					1229:1241	microplastics	1229:1241	microplastics	1229:1241	Moreover, after more than 70 days of contact with microplastics, the microalgae genes involved in the sugar biosynthesis pathways were strongly over-expressed compared to control conditions.
27236494	8	44	theme	over-expression	1384:1398	arg1	levels					1374:1379	The levels	1370:1379	The levels of over-expression	1370:1398	The levels of over-expression were higher in the case of HDPE than in PP condition.
27236494	8	44	theme	over-expression	1384:1398	arg1	higher					1405:1410	higher	1405:1410	higher	1405:1410	The levels of over-expression were higher in the case of HDPE than in PP condition.
27236494	5	45	theme	fragments	966:974	arg1	%					982:982	50%	980:982	50% of microalgae, which led to a final density close to 1.2	980:1039	An estimation of the hetero-aggregates composition was approximately 50% of PP fragments and 50% of microalgae, which led to a final density close to 1.2.
27236494	5	45	theme	fragments	966:974	arg1	estimation					890:899	An estimation	887:899	An estimation of the hetero-aggregates composition	887:936	An estimation of the hetero-aggregates composition was approximately 50% of PP fragments and 50% of microalgae, which led to a final density close to 1.2.
27236494	5	45	theme	fragments	966:974	arg1	microalgae					987:996	microalgae	987:996	microalgae	987:996	An estimation of the hetero-aggregates composition was approximately 50% of PP fragments and 50% of microalgae, which led to a final density close to 1.2.
27236494	5	45	theme	fragments	966:974	arg1	%					958:958	approximately 50%	942:958	approximately 50% of PP fragments	942:974	An estimation of the hetero-aggregates composition was approximately 50% of PP fragments and 50% of microalgae, which led to a final density close to 1.2.
27236494	5	45	theme	fragments	966:974	arg1	fragments					966:974	PP fragments	963:974	PP fragments	963:974	An estimation of the hetero-aggregates composition was approximately 50% of PP fragments and 50% of microalgae, which led to a final density close to 1.2.
27236494	0	46	with	interactions	13:24	arg1	Hetero-aggregation					54:71	freshwater microalgae: Hetero-aggregation	31:71	freshwater microalgae: Hetero-aggregation	31:71	Microplastic interactions with freshwater microalgae: Hetero-aggregation and changes in plastic density appear strongly dependent on polymer type.
27236494	2	47	theme	first	537:541	arg1	days					543:546	the first days	533:546	the first days of contact	533:557	It was shown that the presence of high concentrations of microplastics with size >400 μm did not directly impact the growth of microalgae in the first days of contact and that the expression of three genes involved in the stress response was not modified after 78 days.
27236494	7	48	theme	microalgae	1248:1257	arg1	genes					1259:1263	the microalgae genes	1244:1263	the microalgae genes involved in the sugar biosynthesis pathways	1244:1307	Moreover, after more than 70 days of contact with microplastics, the microalgae genes involved in the sugar biosynthesis pathways were strongly over-expressed compared to control conditions.
27236494	5	49	theme	composition	926:936	arg1	microalgae					987:996	microalgae	987:996	microalgae	987:996	An estimation of the hetero-aggregates composition was approximately 50% of PP fragments and 50% of microalgae, which led to a final density close to 1.2.
27236494	5	49	theme	composition	926:936	arg1	%					958:958	approximately 50%	942:958	approximately 50% of PP fragments	942:974	An estimation of the hetero-aggregates composition was approximately 50% of PP fragments and 50% of microalgae, which led to a final density close to 1.2.
27236494	5	49	theme	composition	926:936	arg1	estimation					890:899	An estimation	887:899	An estimation of the hetero-aggregates composition	887:936	An estimation of the hetero-aggregates composition was approximately 50% of PP fragments and 50% of microalgae, which led to a final density close to 1.2.
27236494	5	49	theme	composition	926:936	arg1	fragments					966:974	PP fragments	963:974	PP fragments	963:974	An estimation of the hetero-aggregates composition was approximately 50% of PP fragments and 50% of microalgae, which led to a final density close to 1.2.
27236494	9	50	theme	first	1477:1481	arg1	evidence					1483:1490	the first evidence that depending on their chemical nature, microplastics will follow different fates in the environment	1473:1592	the first evidence that depending on their chemical nature, microplastics will follow different fates in the environment	1473:1592	This work presents the first evidence that depending on their chemical nature, microplastics will follow different fates in the environment.
27236494	2	51	from	impact	498:503	arg1	days					543:546	the first days	533:546	the first days of contact	533:557	It was shown that the presence of high concentrations of microplastics with size >400 μm did not directly impact the growth of microalgae in the first days of contact and that the expression of three genes involved in the stress response was not modified after 78 days.
27236494	6	52	theme	vertical	1104:1111	arg1	transport					1113:1121	the vertical transport	1100:1121	the vertical transport of PP microplastics from the water surface to sediment	1100:1176	Such hetero-aggregates appear as an important pathway for the vertical transport of PP microplastics from the water surface to sediment.
27236494	2	53	with	microplastics	449:461	arg1	>400 μm					473:479	size >400 μm	468:479	size >400 μm	468:479	It was shown that the presence of high concentrations of microplastics with size >400 μm did not directly impact the growth of microalgae in the first days of contact and that the expression of three genes involved in the stress response was not modified after 78 days.
27607237	0	0	theme	Infectious	88:97	arg1	Growth					99:104	Infectious Growth	88:104	Infectious Growth	88:104	Glycoside Hydrolase MoGls2 Controls Asexual/Sexual Development, Cell Wall Integrity and Infectious Growth in the Rice Blast Fungus.
27607237	2	1	gly	glycoproteins	381:393	arg1	glycoproteins					381:393	N-linked glycoproteins	372:393	N-linked glycoproteins	372:393	Glycoside hydrolases (GHs) are essential in this process, and are involved in processing of N-linked glycoproteins or degradation of carbohydrate structures.
27607237	3	2	link	N-linked	601:608	arg1	glycans					610:616	N-linked glycans	601:616	N-linked glycans	601:616	Here, we identified and characterized MoGls2 in Magnaporthe oryzae, which is a yeast glucosidase II homolog Gls2 and is required for trimming the final glucose in N-linked glycans and normal cell wall synthesis.
27607237	4	3	theme	sexual	815:820	arg1	reproduction					822:833	sexual reproduction	815:833	sexual reproduction	815:833	Target deletion of MoGLS2 in M. oryzae resulted in a reduced mycelial growth, an increased conidial production, delayed conidial germination and loss the ability of sexual reproduction.
27607237	6	4	theme	cell	1072:1075	arg1	stresses					1082:1089	cell wall stresses	1072:1089	cell wall stresses	1072:1089	Further studies showed that the mutant was less sensitive to salt and osmotic stress, and increased sensitivity to cell wall stresses.
27607237	4	5	theme	reproduction	822:833	arg1	ability					804:810	the ability	800:810	the ability of sexual reproduction	800:833	Target deletion of MoGLS2 in M. oryzae resulted in a reduced mycelial growth, an increased conidial production, delayed conidial germination and loss the ability of sexual reproduction.
27607237	8	6	theme	infectious	1360:1369	arg1	growth					1371:1376	infectious growth	1360:1376	infectious growth	1360:1376	Our results indicate that MoGls2 is a key protein for the growth and development of M. oryzae, involving in the regulation of asexual/sexual development, stress response, cell wall integrity and infectious growth.
27607237	8	7	theme	growth	1371:1376	arg1	regulation					1277:1286	the regulation	1273:1286	the regulation of asexual/sexual development, stress response, cell wall integrity and infectious growth	1273:1376	Our results indicate that MoGls2 is a key protein for the growth and development of M. oryzae, involving in the regulation of asexual/sexual development, stress response, cell wall integrity and infectious growth.
27607237	5	8	theme	infectious	938:947	arg1	growth					949:954	infectious growth	938:954	infectious growth	938:954	Pathogenicity assays revealed that the ΔMogls2 mutant showed significantly decreased in virulence and infectious growth.
27607237	8	9	theme	response	1326:1333	arg1	regulation					1277:1286	the regulation	1273:1286	the regulation of asexual/sexual development, stress response, cell wall integrity and infectious growth	1273:1376	Our results indicate that MoGls2 is a key protein for the growth and development of M. oryzae, involving in the regulation of asexual/sexual development, stress response, cell wall integrity and infectious growth.
27607237	8	10	theme	stress	1319:1324	arg1	response					1326:1333	stress response	1319:1333	stress response	1319:1333	Our results indicate that MoGls2 is a key protein for the growth and development of M. oryzae, involving in the regulation of asexual/sexual development, stress response, cell wall integrity and infectious growth.
27607237	4	11	theme	mycelial	711:718	arg1	growth					720:725	a reduced mycelial growth	701:725	a reduced mycelial growth	701:725	Target deletion of MoGLS2 in M. oryzae resulted in a reduced mycelial growth, an increased conidial production, delayed conidial germination and loss the ability of sexual reproduction.
27607237	3	12	theme	yeast	517:521	arg1	which					506:510	which	506:510	which	506:510	Here, we identified and characterized MoGls2 in Magnaporthe oryzae, which is a yeast glucosidase II homolog Gls2 and is required for trimming the final glucose in N-linked glycans and normal cell wall synthesis.
27607237	3	12	theme	yeast	517:521	arg1	Gls2					546:549	a yeast glucosidase II homolog Gls2	515:549	a yeast glucosidase II homolog Gls2	515:549	Here, we identified and characterized MoGls2 in Magnaporthe oryzae, which is a yeast glucosidase II homolog Gls2 and is required for trimming the final glucose in N-linked glycans and normal cell wall synthesis.
27607237	0	13	theme	Rice	113:116	arg1	Fungus					124:129	the Rice Blast Fungus	109:129	the Rice Blast Fungus	109:129	Glycoside Hydrolase MoGls2 Controls Asexual/Sexual Development, Cell Wall Integrity and Infectious Growth in the Rice Blast Fungus.
27607237	7	14	from	defect	1134:1139	arg1	integrity					1154:1162	cell wall integrity	1144:1162	cell wall integrity	1144:1162	Additionally, the ΔMogls2 mutant showed a defect in cell wall integrity.
27607237	2	15	from	essential	311:319	arg1	process					329:335	this process	324:335	this process	324:335	Glycoside hydrolases (GHs) are essential in this process, and are involved in processing of N-linked glycoproteins or degradation of carbohydrate structures.
27607237	8	16	theme	development	1306:1316	arg1	regulation					1277:1286	the regulation	1273:1286	the regulation of asexual/sexual development, stress response, cell wall integrity and infectious growth	1273:1376	Our results indicate that MoGls2 is a key protein for the growth and development of M. oryzae, involving in the regulation of asexual/sexual development, stress response, cell wall integrity and infectious growth.
27607237	0	17	from	Development	51:61	arg1	Fungus					124:129	the Rice Blast Fungus	109:129	the Rice Blast Fungus	109:129	Glycoside Hydrolase MoGls2 Controls Asexual/Sexual Development, Cell Wall Integrity and Infectious Growth in the Rice Blast Fungus.
27607237	2	18	from	process	329:335	arg1	essential					311:319	essential	311:319	essential	311:319	Glycoside hydrolases (GHs) are essential in this process, and are involved in processing of N-linked glycoproteins or degradation of carbohydrate structures.
27607237	2	19	theme	carbohydrate	413:424	arg1	structures					426:435	carbohydrate structures	413:435	carbohydrate structures	413:435	Glycoside hydrolases (GHs) are essential in this process, and are involved in processing of N-linked glycoproteins or degradation of carbohydrate structures.
27607237	1	20	theme	glycosylation	167:179	arg1	glycosylation					141:153	N-linked glycosylation	132:153	N-linked glycosylation	132:153	N-linked glycosylation is a way of glycosylation for newly synthesized protein, which plays a key role in the maturation and transport of proteins.
27607237	1	20	theme	glycosylation	167:179	arg1	way					160:162	a way	158:162	a way of glycosylation for newly synthesized protein, which plays a key role in the maturation and transport of proteins	158:277	N-linked glycosylation is a way of glycosylation for newly synthesized protein, which plays a key role in the maturation and transport of proteins.
27607237	2	21	theme	structures	426:435	arg1	degradation					398:408	degradation	398:408	degradation of carbohydrate structures	398:435	Glycoside hydrolases (GHs) are essential in this process, and are involved in processing of N-linked glycoproteins or degradation of carbohydrate structures.
27607237	2	21	theme	structures	426:435	arg1	processing					358:367	processing	358:367	processing of N-linked glycoproteins	358:393	Glycoside hydrolases (GHs) are essential in this process, and are involved in processing of N-linked glycoproteins or degradation of carbohydrate structures.
27607237	3	22	theme	homolog	538:544	arg1	which					506:510	which	506:510	which	506:510	Here, we identified and characterized MoGls2 in Magnaporthe oryzae, which is a yeast glucosidase II homolog Gls2 and is required for trimming the final glucose in N-linked glycans and normal cell wall synthesis.
27607237	3	22	theme	homolog	538:544	arg1	Gls2					546:549	a yeast glucosidase II homolog Gls2	515:549	a yeast glucosidase II homolog Gls2	515:549	Here, we identified and characterized MoGls2 in Magnaporthe oryzae, which is a yeast glucosidase II homolog Gls2 and is required for trimming the final glucose in N-linked glycans and normal cell wall synthesis.
27607237	3	23	from	glucose	590:596	arg1	glycans					610:616	N-linked glycans	601:616	N-linked glycans	601:616	Here, we identified and characterized MoGls2 in Magnaporthe oryzae, which is a yeast glucosidase II homolog Gls2 and is required for trimming the final glucose in N-linked glycans and normal cell wall synthesis.
27607237	3	23	from	glucose	590:596	arg1	synthesis					639:647	normal cell wall synthesis	622:647	normal cell wall synthesis	622:647	Here, we identified and characterized MoGls2 in Magnaporthe oryzae, which is a yeast glucosidase II homolog Gls2 and is required for trimming the final glucose in N-linked glycans and normal cell wall synthesis.
27607237	0	24	theme	Glycoside	0:8	arg1	MoGls2					20:25	Glycoside Hydrolase MoGls2	0:25	Glycoside Hydrolase MoGls2	0:25	Glycoside Hydrolase MoGls2 Controls Asexual/Sexual Development, Cell Wall Integrity and Infectious Growth in the Rice Blast Fungus.
27607237	6	25	theme	Further	957:963	arg1	studies					965:971	Further studies	957:971	Further studies	957:971	Further studies showed that the mutant was less sensitive to salt and osmotic stress, and increased sensitivity to cell wall stresses.
27607237	3	26	theme	cell	629:632	arg1	synthesis					639:647	normal cell wall synthesis	622:647	normal cell wall synthesis	622:647	Here, we identified and characterized MoGls2 in Magnaporthe oryzae, which is a yeast glucosidase II homolog Gls2 and is required for trimming the final glucose in N-linked glycans and normal cell wall synthesis.
27607237	3	27	theme	wall	634:637	arg1	synthesis					639:647	normal cell wall synthesis	622:647	normal cell wall synthesis	622:647	Here, we identified and characterized MoGls2 in Magnaporthe oryzae, which is a yeast glucosidase II homolog Gls2 and is required for trimming the final glucose in N-linked glycans and normal cell wall synthesis.
27607237	5	28	theme	Pathogenicity	836:848	arg1	assays					850:855	Pathogenicity assays	836:855	Pathogenicity assays	836:855	Pathogenicity assays revealed that the ΔMogls2 mutant showed significantly decreased in virulence and infectious growth.
27607237	3	29	theme	glucosidase	523:533	arg1	which					506:510	which	506:510	which	506:510	Here, we identified and characterized MoGls2 in Magnaporthe oryzae, which is a yeast glucosidase II homolog Gls2 and is required for trimming the final glucose in N-linked glycans and normal cell wall synthesis.
27607237	3	29	theme	glucosidase	523:533	arg1	Gls2					546:549	a yeast glucosidase II homolog Gls2	515:549	a yeast glucosidase II homolog Gls2	515:549	Here, we identified and characterized MoGls2 in Magnaporthe oryzae, which is a yeast glucosidase II homolog Gls2 and is required for trimming the final glucose in N-linked glycans and normal cell wall synthesis.
27607237	8	30	theme	wall	1341:1344	arg1	integrity					1346:1354	cell wall integrity	1336:1354	cell wall integrity	1336:1354	Our results indicate that MoGls2 is a key protein for the growth and development of M. oryzae, involving in the regulation of asexual/sexual development, stress response, cell wall integrity and infectious growth.
27607237	1	31	theme	synthesized	191:201	arg1	protein					203:209	newly synthesized protein	185:209	newly synthesized protein	185:209	N-linked glycosylation is a way of glycosylation for newly synthesized protein, which plays a key role in the maturation and transport of proteins.
27607237	3	32	dep	Magnaporthe	486:496	arg1	required					558:565	required	558:565	is required for trimming the final glucose in N-linked glycans and normal cell wall synthesis	555:647	Here, we identified and characterized MoGls2 in Magnaporthe oryzae, which is a yeast glucosidase II homolog Gls2 and is required for trimming the final glucose in N-linked glycans and normal cell wall synthesis.
27607237	3	32	dep	Magnaporthe	486:496	arg1	which					506:510	which	506:510	which	506:510	Here, we identified and characterized MoGls2 in Magnaporthe oryzae, which is a yeast glucosidase II homolog Gls2 and is required for trimming the final glucose in N-linked glycans and normal cell wall synthesis.
27607237	3	32	dep	Magnaporthe	486:496	arg1	oryzae					498:503	Magnaporthe oryzae, which is a yeast glucosidase II homolog Gls2 and is required for trimming the final glucose in N-linked glycans and normal cell wall synthesis	486:647	oryzae	498:503	Here, we identified and characterized MoGls2 in Magnaporthe oryzae, which is a yeast glucosidase II homolog Gls2 and is required for trimming the final glucose in N-linked glycans and normal cell wall synthesis.
27607237	3	32	dep	Magnaporthe	486:496	arg1	Gls2					546:549	a yeast glucosidase II homolog Gls2	515:549	a yeast glucosidase II homolog Gls2	515:549	Here, we identified and characterized MoGls2 in Magnaporthe oryzae, which is a yeast glucosidase II homolog Gls2 and is required for trimming the final glucose in N-linked glycans and normal cell wall synthesis.
27607237	1	33	theme	proteins	270:277	arg1	transport					257:265	transport	257:265	transport	257:265	N-linked glycosylation is a way of glycosylation for newly synthesized protein, which plays a key role in the maturation and transport of proteins.
27607237	1	33	theme	proteins	270:277	arg1	maturation					242:251	maturation	242:251	maturation	242:251	N-linked glycosylation is a way of glycosylation for newly synthesized protein, which plays a key role in the maturation and transport of proteins.
27607237	0	34	theme	Blast	118:122	arg1	Fungus					124:129	the Rice Blast Fungus	109:129	the Rice Blast Fungus	109:129	Glycoside Hydrolase MoGls2 Controls Asexual/Sexual Development, Cell Wall Integrity and Infectious Growth in the Rice Blast Fungus.
27607237	2	35	theme	Glycoside	280:288	arg1	hydrolases					290:299	Glycoside hydrolases	280:299	Glycoside hydrolases (GHs)	280:305	Glycoside hydrolases (GHs) are essential in this process, and are involved in processing of N-linked glycoproteins or degradation of carbohydrate structures.
27607237	2	35	theme	Glycoside	280:288	arg1	GHs					302:304	GHs	302:304	GHs	302:304	Glycoside hydrolases (GHs) are essential in this process, and are involved in processing of N-linked glycoproteins or degradation of carbohydrate structures.
27607237	2	36	theme	glycoproteins	381:393	arg1	degradation					398:408	degradation	398:408	degradation of carbohydrate structures	398:435	Glycoside hydrolases (GHs) are essential in this process, and are involved in processing of N-linked glycoproteins or degradation of carbohydrate structures.
27607237	2	36	theme	glycoproteins	381:393	arg1	processing					358:367	processing	358:367	processing of N-linked glycoproteins	358:393	Glycoside hydrolases (GHs) are essential in this process, and are involved in processing of N-linked glycoproteins or degradation of carbohydrate structures.
27607237	7	37	theme	cell	1144:1147	arg1	integrity					1154:1162	cell wall integrity	1144:1162	cell wall integrity	1144:1162	Additionally, the ΔMogls2 mutant showed a defect in cell wall integrity.
27607237	4	38	theme	conidial	770:777	arg1	germination					779:789	delayed conidial germination	762:789	delayed conidial germination	762:789	Target deletion of MoGLS2 in M. oryzae resulted in a reduced mycelial growth, an increased conidial production, delayed conidial germination and loss the ability of sexual reproduction.
27607237	8	39	theme	key	1203:1205	arg1	protein					1207:1213	a key protein	1201:1213	a key protein for the growth and development of M. oryzae	1201:1257	Our results indicate that MoGls2 is a key protein for the growth and development of M. oryzae, involving in the regulation of asexual/sexual development, stress response, cell wall integrity and infectious growth.
27607237	8	39	theme	key	1203:1205	arg1	MoGls2					1191:1196	MoGls2	1191:1196	MoGls2	1191:1196	Our results indicate that MoGls2 is a key protein for the growth and development of M. oryzae, involving in the regulation of asexual/sexual development, stress response, cell wall integrity and infectious growth.
27607237	2	40	theme	N-linked	372:379	arg1	glycoproteins					381:393	N-linked glycoproteins	372:393	N-linked glycoproteins	372:393	Glycoside hydrolases (GHs) are essential in this process, and are involved in processing of N-linked glycoproteins or degradation of carbohydrate structures.
27607237	0	41	theme	Asexual/Sexual	36:49	arg1	Development					51:61	Asexual/Sexual Development	36:61	Asexual/Sexual Development	36:61	Glycoside Hydrolase MoGls2 Controls Asexual/Sexual Development, Cell Wall Integrity and Infectious Growth in the Rice Blast Fungus.
27607237	0	42	from	Integrity	74:82	arg1	Fungus					124:129	the Rice Blast Fungus	109:129	the Rice Blast Fungus	109:129	Glycoside Hydrolase MoGls2 Controls Asexual/Sexual Development, Cell Wall Integrity and Infectious Growth in the Rice Blast Fungus.
27607237	4	43	theme	delayed	762:768	arg1	germination					779:789	delayed conidial germination	762:789	delayed conidial germination	762:789	Target deletion of MoGLS2 in M. oryzae resulted in a reduced mycelial growth, an increased conidial production, delayed conidial germination and loss the ability of sexual reproduction.
27607237	0	44	theme	Cell	64:67	arg1	Integrity					74:82	Cell Wall Integrity	64:82	Cell Wall Integrity	64:82	Glycoside Hydrolase MoGls2 Controls Asexual/Sexual Development, Cell Wall Integrity and Infectious Growth in the Rice Blast Fungus.
27607237	5	45	dep	showed	890:895	arg1	decreased					911:919	decreased	911:919	showed significantly decreased in virulence and infectious growth	890:954	Pathogenicity assays revealed that the ΔMogls2 mutant showed significantly decreased in virulence and infectious growth.
27607237	3	46	theme	N-linked	601:608	arg1	glycans					610:616	N-linked glycans	601:616	N-linked glycans	601:616	Here, we identified and characterized MoGls2 in Magnaporthe oryzae, which is a yeast glucosidase II homolog Gls2 and is required for trimming the final glucose in N-linked glycans and normal cell wall synthesis.
27607237	6	47	theme	wall	1077:1080	arg1	stresses					1082:1089	cell wall stresses	1072:1089	cell wall stresses	1072:1089	Further studies showed that the mutant was less sensitive to salt and osmotic stress, and increased sensitivity to cell wall stresses.
27607237	5	48	theme	ΔMogls2	875:881	arg1	mutant					883:888	the ΔMogls2 mutant	871:888	the ΔMogls2 mutant	871:888	Pathogenicity assays revealed that the ΔMogls2 mutant showed significantly decreased in virulence and infectious growth.
27607237	7	49	theme	ΔMogls2	1110:1116	arg1	mutant					1118:1123	the ΔMogls2 mutant	1106:1123	the ΔMogls2 mutant	1106:1123	Additionally, the ΔMogls2 mutant showed a defect in cell wall integrity.
27607237	1	50	link	N-linked	132:139	arg1	glycosylation					141:153	N-linked glycosylation	132:153	N-linked glycosylation	132:153	N-linked glycosylation is a way of glycosylation for newly synthesized protein, which plays a key role in the maturation and transport of proteins.
27607237	1	50	link	N-linked	132:139	arg1	way					160:162	a way	158:162	a way of glycosylation for newly synthesized protein, which plays a key role in the maturation and transport of proteins	158:277	N-linked glycosylation is a way of glycosylation for newly synthesized protein, which plays a key role in the maturation and transport of proteins.
27607237	2	51	link	N-linked	372:379	arg1	glycoproteins					381:393	N-linked glycoproteins	372:393	N-linked glycoproteins	372:393	Glycoside hydrolases (GHs) are essential in this process, and are involved in processing of N-linked glycoproteins or degradation of carbohydrate structures.
27607237	8	52	theme	integrity	1346:1354	arg1	regulation					1277:1286	the regulation	1273:1286	the regulation of asexual/sexual development, stress response, cell wall integrity and infectious growth	1273:1376	Our results indicate that MoGls2 is a key protein for the growth and development of M. oryzae, involving in the regulation of asexual/sexual development, stress response, cell wall integrity and infectious growth.
27607237	4	53	theme	MoGLS2	669:674	arg1	deletion					657:664	Target deletion	650:664	Target deletion of MoGLS2 in M. oryzae	650:687	Target deletion of MoGLS2 in M. oryzae resulted in a reduced mycelial growth, an increased conidial production, delayed conidial germination and loss the ability of sexual reproduction.
27607237	4	54	theme	conidial	741:748	arg1	production					750:759	an increased conidial production	728:759	an increased conidial production	728:759	Target deletion of MoGLS2 in M. oryzae resulted in a reduced mycelial growth, an increased conidial production, delayed conidial germination and loss the ability of sexual reproduction.
27607237	3	55	theme	final	584:588	arg1	glucose					590:596	the final glucose	580:596	the final glucose in N-linked glycans and normal cell wall synthesis	580:647	Here, we identified and characterized MoGls2 in Magnaporthe oryzae, which is a yeast glucosidase II homolog Gls2 and is required for trimming the final glucose in N-linked glycans and normal cell wall synthesis.
27607237	8	56	theme	cell	1336:1339	arg1	integrity					1346:1354	cell wall integrity	1336:1354	cell wall integrity	1336:1354	Our results indicate that MoGls2 is a key protein for the growth and development of M. oryzae, involving in the regulation of asexual/sexual development, stress response, cell wall integrity and infectious growth.
27607237	8	57	theme	oryzae	1252:1257	arg1	development					1234:1244	development	1234:1244	development	1234:1244	Our results indicate that MoGls2 is a key protein for the growth and development of M. oryzae, involving in the regulation of asexual/sexual development, stress response, cell wall integrity and infectious growth.
27607237	8	57	theme	oryzae	1252:1257	arg1	growth					1223:1228	growth	1223:1228	growth	1223:1228	Our results indicate that MoGls2 is a key protein for the growth and development of M. oryzae, involving in the regulation of asexual/sexual development, stress response, cell wall integrity and infectious growth.
27607237	4	58	theme	reduced	703:709	arg1	growth					720:725	a reduced mycelial growth	701:725	a reduced mycelial growth	701:725	Target deletion of MoGLS2 in M. oryzae resulted in a reduced mycelial growth, an increased conidial production, delayed conidial germination and loss the ability of sexual reproduction.
27607237	8	59	theme	asexual/sexual	1291:1304	arg1	development					1306:1316	asexual/sexual development	1291:1316	asexual/sexual development	1291:1316	Our results indicate that MoGls2 is a key protein for the growth and development of M. oryzae, involving in the regulation of asexual/sexual development, stress response, cell wall integrity and infectious growth.
27607237	3	60	theme	normal	622:627	arg1	synthesis					639:647	normal cell wall synthesis	622:647	normal cell wall synthesis	622:647	Here, we identified and characterized MoGls2 in Magnaporthe oryzae, which is a yeast glucosidase II homolog Gls2 and is required for trimming the final glucose in N-linked glycans and normal cell wall synthesis.
27607237	4	61	theme	increased	731:739	arg1	production					750:759	an increased conidial production	728:759	an increased conidial production	728:759	Target deletion of MoGLS2 in M. oryzae resulted in a reduced mycelial growth, an increased conidial production, delayed conidial germination and loss the ability of sexual reproduction.
27607237	1	62	theme	N-linked	132:139	arg1	glycosylation					141:153	N-linked glycosylation	132:153	N-linked glycosylation	132:153	N-linked glycosylation is a way of glycosylation for newly synthesized protein, which plays a key role in the maturation and transport of proteins.
27607237	1	62	theme	N-linked	132:139	arg1	way					160:162	a way	158:162	a way of glycosylation for newly synthesized protein, which plays a key role in the maturation and transport of proteins	158:277	N-linked glycosylation is a way of glycosylation for newly synthesized protein, which plays a key role in the maturation and transport of proteins.
27607237	0	63	from	Growth	99:104	arg1	Fungus					124:129	the Rice Blast Fungus	109:129	the Rice Blast Fungus	109:129	Glycoside Hydrolase MoGls2 Controls Asexual/Sexual Development, Cell Wall Integrity and Infectious Growth in the Rice Blast Fungus.
27607237	4	64	from	deletion	657:664	arg1	oryzae					682:687	M. oryzae	679:687	M. oryzae	679:687	Target deletion of MoGLS2 in M. oryzae resulted in a reduced mycelial growth, an increased conidial production, delayed conidial germination and loss the ability of sexual reproduction.
27607237	6	65	theme	osmotic	1027:1033	arg1	stress					1035:1040	osmotic stress	1027:1040	osmotic stress	1027:1040	Further studies showed that the mutant was less sensitive to salt and osmotic stress, and increased sensitivity to cell wall stresses.
27607237	4	66	theme	Target	650:655	arg1	deletion					657:664	Target deletion	650:664	Target deletion of MoGLS2 in M. oryzae	650:687	Target deletion of MoGLS2 in M. oryzae resulted in a reduced mycelial growth, an increased conidial production, delayed conidial germination and loss the ability of sexual reproduction.
27607237	1	67	theme	key	226:228	arg1	role					230:233	a key role	224:233	a key role	224:233	N-linked glycosylation is a way of glycosylation for newly synthesized protein, which plays a key role in the maturation and transport of proteins.
27607237	0	68	theme	Wall	69:72	arg1	Integrity					74:82	Cell Wall Integrity	64:82	Cell Wall Integrity	64:82	Glycoside Hydrolase MoGls2 Controls Asexual/Sexual Development, Cell Wall Integrity and Infectious Growth in the Rice Blast Fungus.
27607237	7	69	theme	wall	1149:1152	arg1	integrity					1154:1162	cell wall integrity	1144:1162	cell wall integrity	1144:1162	Additionally, the ΔMogls2 mutant showed a defect in cell wall integrity.
27607237	0	70	theme	Hydrolase	10:18	arg1	MoGls2					20:25	Glycoside Hydrolase MoGls2	0:25	Glycoside Hydrolase MoGls2	0:25	Glycoside Hydrolase MoGls2 Controls Asexual/Sexual Development, Cell Wall Integrity and Infectious Growth in the Rice Blast Fungus.
26774420	7	0	theme	ATCC	983:986	arg1	cluster					919:925	the cluster	915:925	the cluster of Acidipila rosea AP8(T) and Acidobacterium capsulatum ATCC 51196(T)	915:995	According to the 16S rRNA gene sequence analysis, strain S15(T) belongs to subdivision 1 of the phylum Acidobacteria, being affiliated to the cluster of Acidipila rosea AP8(T) and Acidobacterium capsulatum ATCC 51196(T).
26774420	5	1	from	22-24°C.	655:662	arg1	growth					645:650	optimum growth	637:650	optimum growth at 22-24°C. Its respiratory quinone	637:686	It can grow at temperatures between 20°C and 28°C, with optimum growth at 22-24°C. Its respiratory quinone is MK-8, and its main fatty acid is iso-C15:0 (73.7%).
26774420	9	2	theme	name	1359:1362	arg1	nov.					1394:1397	the name Silvibacterium bohemicum gen. nov.	1355:1397	the name Silvibacterium bohemicum gen. nov.	1355:1397	Based on genotypic and phenotypic characteristics, the strain S15(T) represents a new genus and species within the family Acidobacteriaceae, for which the name Silvibacterium bohemicum gen. nov., sp.
26774420	7	3	theme	16S	794:796	arg1	analysis					817:824	the 16S rRNA gene sequence analysis	790:824	the 16S rRNA gene sequence analysis	790:824	According to the 16S rRNA gene sequence analysis, strain S15(T) belongs to subdivision 1 of the phylum Acidobacteria, being affiliated to the cluster of Acidipila rosea AP8(T) and Acidobacterium capsulatum ATCC 51196(T).
26774420	2	4	theme	strain	298:303	arg1	T					309:309	T	309:309	T	309:309	During the course of a study assessing the bacterial diversity of a coniferous forest soil (pH 3.8) in the Bohemian Forest National Park (Czech Republic), we isolated strain S15(T) which corresponded to one of the most abundant soil OTUs.
26774420	2	4	theme	strain	298:303	arg1	S15					305:307	strain S15	298:307	strain S15(T) which corresponded to one of the most abundant soil OTUs	298:367	During the course of a study assessing the bacterial diversity of a coniferous forest soil (pH 3.8) in the Bohemian Forest National Park (Czech Republic), we isolated strain S15(T) which corresponded to one of the most abundant soil OTUs.
26774420	8	5	theme	plant	1179:1183	arg1	origin					1196:1201	plant and fungal origin	1179:1201	origin	1196:1201	Analysis of the S15(T) genome revealed the presence of 404 genes that are involved in carbohydrate metabolism, which indicates the metabolic potential to degrade polysaccharides of plant and fungal origin.
26774420	9	6	theme	Silvibacterium	1364:1377	arg1	nov.					1394:1397	the name Silvibacterium bohemicum gen. nov.	1355:1397	the name Silvibacterium bohemicum gen. nov.	1355:1397	Based on genotypic and phenotypic characteristics, the strain S15(T) represents a new genus and species within the family Acidobacteriaceae, for which the name Silvibacterium bohemicum gen. nov., sp.
26774420	3	7	theme	Gram-negative	402:414	arg1	cells					434:438	Gram-negative, motile, rod-like cells	402:438	Gram-negative, motile, rod-like cells that are 0.3-0.5μm in diameter and 0.9-1.1μm in length	402:493	Strain S15(T) is represented by Gram-negative, motile, rod-like cells that are 0.3-0.5μm in diameter and 0.9-1.1μm in length.
26774420	7	8	theme	rosea	940:944	arg1	T					994:994	T	994:994	T	994:994	According to the 16S rRNA gene sequence analysis, strain S15(T) belongs to subdivision 1 of the phylum Acidobacteria, being affiliated to the cluster of Acidipila rosea AP8(T) and Acidobacterium capsulatum ATCC 51196(T).
26774420	7	8	theme	rosea	940:944	arg1	T					950:950	Acidipila rosea AP8(T) and Acidobacterium capsulatum ATCC 51196	930:992	T	950:950	According to the 16S rRNA gene sequence analysis, strain S15(T) belongs to subdivision 1 of the phylum Acidobacteria, being affiliated to the cluster of Acidipila rosea AP8(T) and Acidobacterium capsulatum ATCC 51196(T).
26774420	9	9	theme	strain	1259:1264	arg1	T					1270:1270	T	1270:1270	T	1270:1270	Based on genotypic and phenotypic characteristics, the strain S15(T) represents a new genus and species within the family Acidobacteriaceae, for which the name Silvibacterium bohemicum gen. nov., sp.
26774420	9	9	theme	strain	1259:1264	arg1	S15					1266:1268	the strain S15	1255:1268	the strain S15(T)	1255:1271	Based on genotypic and phenotypic characteristics, the strain S15(T) represents a new genus and species within the family Acidobacteriaceae, for which the name Silvibacterium bohemicum gen. nov., sp.
26774420	9	10	theme	bohemicum	1379:1387	arg1	nov.					1394:1397	the name Silvibacterium bohemicum gen. nov.	1355:1397	the name Silvibacterium bohemicum gen. nov.	1355:1397	Based on genotypic and phenotypic characteristics, the strain S15(T) represents a new genus and species within the family Acidobacteriaceae, for which the name Silvibacterium bohemicum gen. nov., sp.
26774420	9	11	theme	family	1319:1324	arg1	Acidobacteriaceae					1326:1342	the family Acidobacteriaceae	1315:1342	the family Acidobacteriaceae	1315:1342	Based on genotypic and phenotypic characteristics, the strain S15(T) represents a new genus and species within the family Acidobacteriaceae, for which the name Silvibacterium bohemicum gen. nov., sp.
26774420	10	12	theme	=CECT	1453:1457	arg1	T					1464:1464	T	1464:1464	T	1464:1464	nov. is proposed (type strain S15(T)=LMG 28607(T)=CECT 8790(T)).
26774420	10	12	theme	=CECT	1453:1457	arg1	8790					1459:1462	type strain S15(T)=LMG 28607(T)=CECT 8790	1422:1462	type strain S15(T)=LMG 28607(T)=CECT 8790(T)	1422:1465	nov. is proposed (type strain S15(T)=LMG 28607(T)=CECT 8790(T)).
26774420	7	13	theme	capsulatum	972:981	arg1	ATCC					983:986	Acidipila rosea AP8(T) and Acidobacterium capsulatum ATCC 51196	930:992	ATCC	983:986	According to the 16S rRNA gene sequence analysis, strain S15(T) belongs to subdivision 1 of the phylum Acidobacteria, being affiliated to the cluster of Acidipila rosea AP8(T) and Acidobacterium capsulatum ATCC 51196(T).
26774420	4	14	located	found	553:557	arg2	conditions					542:551	optimal conditions	534:551	optimal conditions found at approximately 4-5	534:578	Its pH range for growth was 3-6, with optimal conditions found at approximately 4-5.
26774420	4	14	located	found	553:557	arg1	4-5					576:578	4-5	576:578	4-5	576:578	Its pH range for growth was 3-6, with optimal conditions found at approximately 4-5.
26774420	9	15	theme	gen.	1389:1392	arg1	nov.					1394:1397	the name Silvibacterium bohemicum gen. nov.	1355:1397	the name Silvibacterium bohemicum gen. nov.	1355:1397	Based on genotypic and phenotypic characteristics, the strain S15(T) represents a new genus and species within the family Acidobacteriaceae, for which the name Silvibacterium bohemicum gen. nov., sp.
26774420	8	16	theme	genes	1057:1061	arg1	presence					1041:1048	the presence	1037:1048	the presence of 404 genes that are involved in carbohydrate metabolism, which indicates the metabolic potential to degrade polysaccharides of plant and fungal origin	1037:1201	Analysis of the S15(T) genome revealed the presence of 404 genes that are involved in carbohydrate metabolism, which indicates the metabolic potential to degrade polysaccharides of plant and fungal origin.
26774420	7	17	theme	phylum	873:878	arg1	Acidobacteria					880:892	the phylum Acidobacteria	869:892	the phylum Acidobacteria	869:892	According to the 16S rRNA gene sequence analysis, strain S15(T) belongs to subdivision 1 of the phylum Acidobacteria, being affiliated to the cluster of Acidipila rosea AP8(T) and Acidobacterium capsulatum ATCC 51196(T).
26774420	5	18	theme	main	705:708	arg1	acid					716:719	its main fatty acid	701:719	its main fatty acid	701:719	It can grow at temperatures between 20°C and 28°C, with optimum growth at 22-24°C. Its respiratory quinone is MK-8, and its main fatty acid is iso-C15:0 (73.7%).
26774420	2	19	from	diversity	184:192	arg1	Park					263:266	Park	263:266	Park	263:266	During the course of a study assessing the bacterial diversity of a coniferous forest soil (pH 3.8) in the Bohemian Forest National Park (Czech Republic), we isolated strain S15(T) which corresponded to one of the most abundant soil OTUs.
26774420	2	19	from	diversity	184:192	arg1	Republic					275:282	Republic	275:282	Republic	275:282	During the course of a study assessing the bacterial diversity of a coniferous forest soil (pH 3.8) in the Bohemian Forest National Park (Czech Republic), we isolated strain S15(T) which corresponded to one of the most abundant soil OTUs.
26774420	5	20	dep	22-24°C.	655:662	arg1	quinone					680:686	Its respiratory quinone	664:686	22-24°C. Its respiratory quinone	655:686	It can grow at temperatures between 20°C and 28°C, with optimum growth at 22-24°C. Its respiratory quinone is MK-8, and its main fatty acid is iso-C15:0 (73.7%).
26774420	0	21	theme	Silvibacterium	0:13	arg1	nov.					30:33	Silvibacterium bohemicum gen. nov.	0:33	Silvibacterium bohemicum gen. nov.	0:33	Silvibacterium bohemicum gen. nov. sp.
26774420	5	22	theme	fatty	710:714	arg1	acid					716:719	its main fatty acid	701:719	its main fatty acid	701:719	It can grow at temperatures between 20°C and 28°C, with optimum growth at 22-24°C. Its respiratory quinone is MK-8, and its main fatty acid is iso-C15:0 (73.7%).
26774420	0	23	theme	bohemicum	15:23	arg1	nov.					30:33	Silvibacterium bohemicum gen. nov.	0:33	Silvibacterium bohemicum gen. nov.	0:33	Silvibacterium bohemicum gen. nov. sp.
26774420	10	24	dep	proposed	1412:1419	arg1	T					1464:1464	T	1464:1464	T	1464:1464	nov. is proposed (type strain S15(T)=LMG 28607(T)=CECT 8790(T)).
26774420	10	24	dep	proposed	1412:1419	arg1	8790					1459:1462	type strain S15(T)=LMG 28607(T)=CECT 8790	1422:1462	type strain S15(T)=LMG 28607(T)=CECT 8790(T)	1422:1465	nov. is proposed (type strain S15(T)=LMG 28607(T)=CECT 8790(T)).
26774420	1	25	theme	coniferous	77:86	arg1	soil					88:91	coniferous soil	77:91	coniferous soil in the Bohemian Forest National Park	77:128	nov., an acidobacterium isolated from coniferous soil in the Bohemian Forest National Park.
26774420	2	26	theme	soil	359:362	arg1	OTUs					364:367	the most abundant soil OTUs	341:367	the most abundant soil OTUs	341:367	During the course of a study assessing the bacterial diversity of a coniferous forest soil (pH 3.8) in the Bohemian Forest National Park (Czech Republic), we isolated strain S15(T) which corresponded to one of the most abundant soil OTUs.
26774420	9	27	theme	new	1286:1288	arg1	genus					1290:1294	a new genus	1284:1294	a new genus	1284:1294	Based on genotypic and phenotypic characteristics, the strain S15(T) represents a new genus and species within the family Acidobacteriaceae, for which the name Silvibacterium bohemicum gen. nov., sp.
26774420	3	28	dep	Gram-negative	402:414	arg1	rod-like					425:432	rod-like	425:432	rod-like	425:432	Strain S15(T) is represented by Gram-negative, motile, rod-like cells that are 0.3-0.5μm in diameter and 0.9-1.1μm in length.
26774420	3	28	dep	Gram-negative	402:414	arg1	motile					417:422	motile	417:422	motile	417:422	Strain S15(T) is represented by Gram-negative, motile, rod-like cells that are 0.3-0.5μm in diameter and 0.9-1.1μm in length.
26774420	4	29	theme	optimal	534:540	arg1	conditions					542:551	optimal conditions	534:551	optimal conditions found at approximately 4-5	534:578	Its pH range for growth was 3-6, with optimal conditions found at approximately 4-5.
26774420	2	30	theme	abundant	350:357	arg1	OTUs					364:367	the most abundant soil OTUs	341:367	the most abundant soil OTUs	341:367	During the course of a study assessing the bacterial diversity of a coniferous forest soil (pH 3.8) in the Bohemian Forest National Park (Czech Republic), we isolated strain S15(T) which corresponded to one of the most abundant soil OTUs.
26774420	5	31	theme	respiratory	668:678	arg1	quinone					680:686	Its respiratory quinone	664:686	22-24°C. Its respiratory quinone	655:686	It can grow at temperatures between 20°C and 28°C, with optimum growth at 22-24°C. Its respiratory quinone is MK-8, and its main fatty acid is iso-C15:0 (73.7%).
26774420	8	32	theme	metabolic	1129:1137	arg1	potential					1139:1147	the metabolic potential	1125:1147	the metabolic potential to degrade polysaccharides of plant and fungal origin	1125:1201	Analysis of the S15(T) genome revealed the presence of 404 genes that are involved in carbohydrate metabolism, which indicates the metabolic potential to degrade polysaccharides of plant and fungal origin.
26774420	10	33	theme	T	1451:1451	arg1	T					1464:1464	T	1464:1464	T	1464:1464	nov. is proposed (type strain S15(T)=LMG 28607(T)=CECT 8790(T)).
26774420	10	33	theme	T	1451:1451	arg1	8790					1459:1462	type strain S15(T)=LMG 28607(T)=CECT 8790	1422:1462	type strain S15(T)=LMG 28607(T)=CECT 8790(T)	1422:1465	nov. is proposed (type strain S15(T)=LMG 28607(T)=CECT 8790(T)).
26774420	3	34	from	0.9-1.1μm	475:483	arg1	0.3-0.5μm					449:457	0.3-0.5μm	449:457	0.3-0.5μm	449:457	Strain S15(T) is represented by Gram-negative, motile, rod-like cells that are 0.3-0.5μm in diameter and 0.9-1.1μm in length.
26774420	3	34	from	0.9-1.1μm	475:483	arg1	length					488:493	length	488:493	length	488:493	Strain S15(T) is represented by Gram-negative, motile, rod-like cells that are 0.3-0.5μm in diameter and 0.9-1.1μm in length.
26774420	8	35	theme	S15	1014:1016	arg1	genome					1021:1026	the S15(T) genome	1010:1026	the S15(T) genome	1010:1026	Analysis of the S15(T) genome revealed the presence of 404 genes that are involved in carbohydrate metabolism, which indicates the metabolic potential to degrade polysaccharides of plant and fungal origin.
26774420	0	36	theme	gen.	25:28	arg1	nov.					30:33	Silvibacterium bohemicum gen. nov.	0:33	Silvibacterium bohemicum gen. nov.	0:33	Silvibacterium bohemicum gen. nov. sp.
26774420	10	37	theme	type	1422:1425	arg1	T					1464:1464	T	1464:1464	T	1464:1464	nov. is proposed (type strain S15(T)=LMG 28607(T)=CECT 8790(T)).
26774420	10	37	theme	type	1422:1425	arg1	8790					1459:1462	type strain S15(T)=LMG 28607(T)=CECT 8790	1422:1462	type strain S15(T)=LMG 28607(T)=CECT 8790(T)	1422:1465	nov. is proposed (type strain S15(T)=LMG 28607(T)=CECT 8790(T)).
26774420	10	38	theme	=LMG	1440:1443	arg1	T					1464:1464	T	1464:1464	T	1464:1464	nov. is proposed (type strain S15(T)=LMG 28607(T)=CECT 8790(T)).
26774420	10	38	theme	=LMG	1440:1443	arg1	8790					1459:1462	type strain S15(T)=LMG 28607(T)=CECT 8790	1422:1462	type strain S15(T)=LMG 28607(T)=CECT 8790(T)	1422:1465	nov. is proposed (type strain S15(T)=LMG 28607(T)=CECT 8790(T)).
26774420	5	39	theme	optimum	637:643	arg1	growth					645:650	optimum growth	637:650	optimum growth at 22-24°C. Its respiratory quinone	637:686	It can grow at temperatures between 20°C and 28°C, with optimum growth at 22-24°C. Its respiratory quinone is MK-8, and its main fatty acid is iso-C15:0 (73.7%).
26774420	1	40	attach	isolated	63:70	arg1	soil					88:91	coniferous soil	77:91	coniferous soil in the Bohemian Forest National Park	77:128	nov., an acidobacterium isolated from coniferous soil in the Bohemian Forest National Park.
26774420	1	40	attach	isolated	63:70	arg2	acidobacterium					48:61	an acidobacterium	45:61	an acidobacterium isolated from coniferous soil in the Bohemian Forest National Park	45:128	nov., an acidobacterium isolated from coniferous soil in the Bohemian Forest National Park.
26774420	9	41	dep	sp	1400:1401	arg1	nov.					1394:1397	the name Silvibacterium bohemicum gen. nov.	1355:1397	the name Silvibacterium bohemicum gen. nov.	1355:1397	Based on genotypic and phenotypic characteristics, the strain S15(T) represents a new genus and species within the family Acidobacteriaceae, for which the name Silvibacterium bohemicum gen. nov., sp.
26774420	2	42	theme	study	154:158	arg1	course					142:147	the course	138:147	the course of a study assessing the bacterial diversity of a coniferous forest soil (pH 3.8) in the Bohemian Forest National Park (Czech Republic)	138:283	During the course of a study assessing the bacterial diversity of a coniferous forest soil (pH 3.8) in the Bohemian Forest National Park (Czech Republic), we isolated strain S15(T) which corresponded to one of the most abundant soil OTUs.
26774420	2	43	theme	soil	217:220	arg1	diversity					184:192	the bacterial diversity	170:192	the bacterial diversity of a coniferous forest soil (pH 3.8) in the Bohemian Forest National Park (Czech Republic)	170:283	During the course of a study assessing the bacterial diversity of a coniferous forest soil (pH 3.8) in the Bohemian Forest National Park (Czech Republic), we isolated strain S15(T) which corresponded to one of the most abundant soil OTUs.
26774420	1	44	from	soil	88:91	arg1	Park					125:128	Park	125:128	Park	125:128	nov., an acidobacterium isolated from coniferous soil in the Bohemian Forest National Park.
26774420	8	45	theme	T	1018:1018	arg1	genome					1021:1026	the S15(T) genome	1010:1026	the S15(T) genome	1010:1026	Analysis of the S15(T) genome revealed the presence of 404 genes that are involved in carbohydrate metabolism, which indicates the metabolic potential to degrade polysaccharides of plant and fungal origin.
26774420	7	46	theme	Acidipila	930:938	arg1	T					994:994	T	994:994	T	994:994	According to the 16S rRNA gene sequence analysis, strain S15(T) belongs to subdivision 1 of the phylum Acidobacteria, being affiliated to the cluster of Acidipila rosea AP8(T) and Acidobacterium capsulatum ATCC 51196(T).
26774420	7	46	theme	Acidipila	930:938	arg1	T					950:950	Acidipila rosea AP8(T) and Acidobacterium capsulatum ATCC 51196	930:992	T	950:950	According to the 16S rRNA gene sequence analysis, strain S15(T) belongs to subdivision 1 of the phylum Acidobacteria, being affiliated to the cluster of Acidipila rosea AP8(T) and Acidobacterium capsulatum ATCC 51196(T).
26774420	6	47	theme	58.2mol	767:773	arg1	content					755:761	The G+C DNA content	743:761	The G+C DNA content	743:761	The G+C DNA content was 58.2mol%.
26774420	6	47	theme	58.2mol	767:773	arg1	%					774:774	58.2mol%	767:774	58.2mol%	767:774	The G+C DNA content was 58.2mol%.
26774420	8	48	theme	origin	1196:1201	arg1	polysaccharides					1160:1174	polysaccharides	1160:1174	polysaccharides of plant and fungal origin	1160:1201	Analysis of the S15(T) genome revealed the presence of 404 genes that are involved in carbohydrate metabolism, which indicates the metabolic potential to degrade polysaccharides of plant and fungal origin.
26774420	2	49	theme	forest	210:215	arg1	pH					223:224	pH 3.8	223:228	pH 3.8	223:228	During the course of a study assessing the bacterial diversity of a coniferous forest soil (pH 3.8) in the Bohemian Forest National Park (Czech Republic), we isolated strain S15(T) which corresponded to one of the most abundant soil OTUs.
26774420	2	49	theme	forest	210:215	arg1	soil					217:220	a coniferous forest soil	197:220	a coniferous forest soil (pH 3.8)	197:229	During the course of a study assessing the bacterial diversity of a coniferous forest soil (pH 3.8) in the Bohemian Forest National Park (Czech Republic), we isolated strain S15(T) which corresponded to one of the most abundant soil OTUs.
26774420	3	50	theme	Strain	370:375	arg1	T					381:381	T	381:381	T	381:381	Strain S15(T) is represented by Gram-negative, motile, rod-like cells that are 0.3-0.5μm in diameter and 0.9-1.1μm in length.
26774420	3	50	theme	Strain	370:375	arg1	S15					377:379	Strain S15	370:379	Strain S15(T)	370:382	Strain S15(T) is represented by Gram-negative, motile, rod-like cells that are 0.3-0.5μm in diameter and 0.9-1.1μm in length.
26774420	7	51	theme	Acidobacterium	957:970	arg1	ATCC					983:986	Acidipila rosea AP8(T) and Acidobacterium capsulatum ATCC 51196	930:992	ATCC	983:986	According to the 16S rRNA gene sequence analysis, strain S15(T) belongs to subdivision 1 of the phylum Acidobacteria, being affiliated to the cluster of Acidipila rosea AP8(T) and Acidobacterium capsulatum ATCC 51196(T).
26774420	8	52	theme	fungal	1189:1194	arg1	origin					1196:1201	plant and fungal origin	1179:1201	origin	1196:1201	Analysis of the S15(T) genome revealed the presence of 404 genes that are involved in carbohydrate metabolism, which indicates the metabolic potential to degrade polysaccharides of plant and fungal origin.
26774420	2	53	theme	coniferous	199:208	arg1	pH					223:224	pH 3.8	223:228	pH 3.8	223:228	During the course of a study assessing the bacterial diversity of a coniferous forest soil (pH 3.8) in the Bohemian Forest National Park (Czech Republic), we isolated strain S15(T) which corresponded to one of the most abundant soil OTUs.
26774420	2	53	theme	coniferous	199:208	arg1	soil					217:220	a coniferous forest soil	197:220	a coniferous forest soil (pH 3.8)	197:229	During the course of a study assessing the bacterial diversity of a coniferous forest soil (pH 3.8) in the Bohemian Forest National Park (Czech Republic), we isolated strain S15(T) which corresponded to one of the most abundant soil OTUs.
26774420	7	54	theme	strain	827:832	arg1	T					838:838	T	838:838	T	838:838	According to the 16S rRNA gene sequence analysis, strain S15(T) belongs to subdivision 1 of the phylum Acidobacteria, being affiliated to the cluster of Acidipila rosea AP8(T) and Acidobacterium capsulatum ATCC 51196(T).
26774420	7	54	theme	strain	827:832	arg1	S15					834:836	strain S15	827:836	strain S15(T)	827:839	According to the 16S rRNA gene sequence analysis, strain S15(T) belongs to subdivision 1 of the phylum Acidobacteria, being affiliated to the cluster of Acidipila rosea AP8(T) and Acidobacterium capsulatum ATCC 51196(T).
26774420	8	55	theme	genome	1021:1026	arg1	Analysis					998:1005	Analysis	998:1005	Analysis of the S15(T) genome	998:1026	Analysis of the S15(T) genome revealed the presence of 404 genes that are involved in carbohydrate metabolism, which indicates the metabolic potential to degrade polysaccharides of plant and fungal origin.
26774420	10	56	theme	S15	1434:1436	arg1	T					1464:1464	T	1464:1464	T	1464:1464	nov. is proposed (type strain S15(T)=LMG 28607(T)=CECT 8790(T)).
26774420	10	56	theme	S15	1434:1436	arg1	8790					1459:1462	type strain S15(T)=LMG 28607(T)=CECT 8790	1422:1462	type strain S15(T)=LMG 28607(T)=CECT 8790(T)	1422:1465	nov. is proposed (type strain S15(T)=LMG 28607(T)=CECT 8790(T)).
26774420	0	57	dep	sp	35:36	arg1	nov.					30:33	Silvibacterium bohemicum gen. nov.	0:33	Silvibacterium bohemicum gen. nov.	0:33	Silvibacterium bohemicum gen. nov. sp.
26774420	7	58	theme	sequence	808:815	arg1	analysis					817:824	the 16S rRNA gene sequence analysis	790:824	the 16S rRNA gene sequence analysis	790:824	According to the 16S rRNA gene sequence analysis, strain S15(T) belongs to subdivision 1 of the phylum Acidobacteria, being affiliated to the cluster of Acidipila rosea AP8(T) and Acidobacterium capsulatum ATCC 51196(T).
26774420	9	59	theme	phenotypic	1227:1236	arg1	characteristics					1238:1252	genotypic and phenotypic characteristics	1213:1252	genotypic and phenotypic characteristics	1213:1252	Based on genotypic and phenotypic characteristics, the strain S15(T) represents a new genus and species within the family Acidobacteriaceae, for which the name Silvibacterium bohemicum gen. nov., sp.
26774420	6	60	theme	DNA	751:753	arg1	content					755:761	The G+C DNA content	743:761	The G+C DNA content	743:761	The G+C DNA content was 58.2mol%.
26774420	6	60	theme	DNA	751:753	arg1	%					774:774	58.2mol%	767:774	58.2mol%	767:774	The G+C DNA content was 58.2mol%.
26774420	8	61	theme	carbohydrate	1084:1095	arg1	metabolism					1097:1106	carbohydrate metabolism	1084:1106	carbohydrate metabolism	1084:1106	Analysis of the S15(T) genome revealed the presence of 404 genes that are involved in carbohydrate metabolism, which indicates the metabolic potential to degrade polysaccharides of plant and fungal origin.
26774420	3	62	from	0.3-0.5μm	449:457	arg1	0.9-1.1μm					475:483	0.9-1.1μm	475:483	0.9-1.1μm	475:483	Strain S15(T) is represented by Gram-negative, motile, rod-like cells that are 0.3-0.5μm in diameter and 0.9-1.1μm in length.
26774420	3	62	from	0.3-0.5μm	449:457	arg1	diameter					462:469	diameter	462:469	diameter	462:469	Strain S15(T) is represented by Gram-negative, motile, rod-like cells that are 0.3-0.5μm in diameter and 0.9-1.1μm in length.
26774420	10	63	theme	T	1438:1438	arg1	T					1464:1464	T	1464:1464	T	1464:1464	nov. is proposed (type strain S15(T)=LMG 28607(T)=CECT 8790(T)).
26774420	10	63	theme	T	1438:1438	arg1	8790					1459:1462	type strain S15(T)=LMG 28607(T)=CECT 8790	1422:1462	type strain S15(T)=LMG 28607(T)=CECT 8790(T)	1422:1465	nov. is proposed (type strain S15(T)=LMG 28607(T)=CECT 8790(T)).
26774420	2	64	theme	bacterial	174:182	arg1	diversity					184:192	the bacterial diversity	170:192	the bacterial diversity of a coniferous forest soil (pH 3.8) in the Bohemian Forest National Park (Czech Republic)	170:283	During the course of a study assessing the bacterial diversity of a coniferous forest soil (pH 3.8) in the Bohemian Forest National Park (Czech Republic), we isolated strain S15(T) which corresponded to one of the most abundant soil OTUs.
26774420	7	65	theme	Acidobacteria	880:892	arg1	subdivision					852:862	subdivision 1	852:864	subdivision 1 of the phylum Acidobacteria	852:892	According to the 16S rRNA gene sequence analysis, strain S15(T) belongs to subdivision 1 of the phylum Acidobacteria, being affiliated to the cluster of Acidipila rosea AP8(T) and Acidobacterium capsulatum ATCC 51196(T).
26774420	7	66	theme	rRNA	798:801	arg1	analysis					817:824	the 16S rRNA gene sequence analysis	790:824	the 16S rRNA gene sequence analysis	790:824	According to the 16S rRNA gene sequence analysis, strain S15(T) belongs to subdivision 1 of the phylum Acidobacteria, being affiliated to the cluster of Acidipila rosea AP8(T) and Acidobacterium capsulatum ATCC 51196(T).
26774420	1	67	dep	acidobacterium	48:61	arg1	nov.					39:42	nov.	39:42	nov.	39:42	nov., an acidobacterium isolated from coniferous soil in the Bohemian Forest National Park.
26774420	9	68	theme	genotypic	1213:1221	arg1	characteristics					1238:1252	genotypic and phenotypic characteristics	1213:1252	genotypic and phenotypic characteristics	1213:1252	Based on genotypic and phenotypic characteristics, the strain S15(T) represents a new genus and species within the family Acidobacteriaceae, for which the name Silvibacterium bohemicum gen. nov., sp.
26774420	7	69	theme	gene	803:806	arg1	analysis					817:824	the 16S rRNA gene sequence analysis	790:824	the 16S rRNA gene sequence analysis	790:824	According to the 16S rRNA gene sequence analysis, strain S15(T) belongs to subdivision 1 of the phylum Acidobacteria, being affiliated to the cluster of Acidipila rosea AP8(T) and Acidobacterium capsulatum ATCC 51196(T).
26774420	10	70	theme	strain	1427:1432	arg1	T					1464:1464	T	1464:1464	T	1464:1464	nov. is proposed (type strain S15(T)=LMG 28607(T)=CECT 8790(T)).
26774420	10	70	theme	strain	1427:1432	arg1	8790					1459:1462	type strain S15(T)=LMG 28607(T)=CECT 8790	1422:1462	type strain S15(T)=LMG 28607(T)=CECT 8790(T)	1422:1465	nov. is proposed (type strain S15(T)=LMG 28607(T)=CECT 8790(T)).
26774420	3	71	from	diameter	462:469	arg1	0.3-0.5μm					449:457	0.3-0.5μm	449:457	0.3-0.5μm	449:457	Strain S15(T) is represented by Gram-negative, motile, rod-like cells that are 0.3-0.5μm in diameter and 0.9-1.1μm in length.
26774420	3	71	from	diameter	462:469	arg1	length					488:493	length	488:493	length	488:493	Strain S15(T) is represented by Gram-negative, motile, rod-like cells that are 0.3-0.5μm in diameter and 0.9-1.1μm in length.
26774420	6	72	theme	G+C	747:749	arg1	content					755:761	The G+C DNA content	743:761	The G+C DNA content	743:761	The G+C DNA content was 58.2mol%.
26774420	6	72	theme	G+C	747:749	arg1	%					774:774	58.2mol%	767:774	58.2mol%	767:774	The G+C DNA content was 58.2mol%.
26774420	7	73	theme	AP8	946:948	arg1	T					994:994	T	994:994	T	994:994	According to the 16S rRNA gene sequence analysis, strain S15(T) belongs to subdivision 1 of the phylum Acidobacteria, being affiliated to the cluster of Acidipila rosea AP8(T) and Acidobacterium capsulatum ATCC 51196(T).
26774420	7	73	theme	AP8	946:948	arg1	T					950:950	Acidipila rosea AP8(T) and Acidobacterium capsulatum ATCC 51196	930:992	T	950:950	According to the 16S rRNA gene sequence analysis, strain S15(T) belongs to subdivision 1 of the phylum Acidobacteria, being affiliated to the cluster of Acidipila rosea AP8(T) and Acidobacterium capsulatum ATCC 51196(T).
26774420	2	74	theme	OTUs	364:367	arg1	one					334:336	one	334:336	one	334:336	During the course of a study assessing the bacterial diversity of a coniferous forest soil (pH 3.8) in the Bohemian Forest National Park (Czech Republic), we isolated strain S15(T) which corresponded to one of the most abundant soil OTUs.
26774420	2	74	theme	OTUs	364:367	arg1	OTUs					364:367	the most abundant soil OTUs	341:367	the most abundant soil OTUs	341:367	During the course of a study assessing the bacterial diversity of a coniferous forest soil (pH 3.8) in the Bohemian Forest National Park (Czech Republic), we isolated strain S15(T) which corresponded to one of the most abundant soil OTUs.
26774420	7	75	theme	T	950:950	arg1	cluster					919:925	the cluster	915:925	the cluster of Acidipila rosea AP8(T) and Acidobacterium capsulatum ATCC 51196(T)	915:995	According to the 16S rRNA gene sequence analysis, strain S15(T) belongs to subdivision 1 of the phylum Acidobacteria, being affiliated to the cluster of Acidipila rosea AP8(T) and Acidobacterium capsulatum ATCC 51196(T).
26774420	4	76	theme	pH	500:501	arg1	range					503:507	Its pH range	496:507	Its pH range for growth	496:518	Its pH range for growth was 3-6, with optimal conditions found at approximately 4-5.
25538055	0	0	theme	composites	87:96	arg1	hemocompatibility					32:48	hemocompatibility	32:48	hemocompatibility	32:48	Structure, physical properties, hemocompatibility and cytocompatibility of starch/zein composites.
25538055	0	0	theme	composites	87:96	arg1	Structure					0:8	Structure	0:8	Structure	0:8	Structure, physical properties, hemocompatibility and cytocompatibility of starch/zein composites.
25538055	0	0	theme	composites	87:96	arg1	properties					20:29	physical properties	11:29	physical properties	11:29	Structure, physical properties, hemocompatibility and cytocompatibility of starch/zein composites.
25538055	0	0	theme	composites	87:96	arg1	cytocompatibility					54:70	cytocompatibility	54:70	cytocompatibility	54:70	Structure, physical properties, hemocompatibility and cytocompatibility of starch/zein composites.
25538055	7	1	theme	SZ	912:913	arg1	maybe					926:930	The SZ composites maybe	908:930	The SZ composites maybe	908:930	The SZ composites maybe have potentials for applications as biomaterials.
25538055	7	2	contain	have	932:935	arg1	maybe					926:930	The SZ composites maybe	908:930	The SZ composites maybe	908:930	The SZ composites maybe have potentials for applications as biomaterials.
25538055	7	2	contain	have	932:935	arg2	potentials					937:946	potentials	937:946	potentials for applications as biomaterials	937:979	The SZ composites maybe have potentials for applications as biomaterials.
25538055	6	3	theme	anticoagulant	844:856	arg1	composites					829:838	SZ composites	826:838	SZ composites	826:838	The hemocompatibility and cytocompatibility evaluation showed that SZ composites are anticoagulant materials with no hemolysis and low cytotoxicity.
25538055	6	3	theme	anticoagulant	844:856	arg1	materials					858:866	anticoagulant materials	844:866	anticoagulant materials with no hemolysis and low cytotoxicity	844:905	The hemocompatibility and cytocompatibility evaluation showed that SZ composites are anticoagulant materials with no hemolysis and low cytotoxicity.
25538055	2	4	theme	films	290:294	arg1	structure					222:230	structure	222:230	structure	222:230	The structure and physical properties of the starch/zein (SZ) composite films were characterized by scanning electron microscope (SEM), optical microscopy and water contract angle testing.
25538055	2	4	theme	films	290:294	arg1	properties					245:254	physical properties	236:254	physical properties	236:254	The structure and physical properties of the starch/zein (SZ) composite films were characterized by scanning electron microscope (SEM), optical microscopy and water contract angle testing.
25538055	7	5	theme	composites	915:924	arg1	maybe					926:930	The SZ composites maybe	908:930	The SZ composites maybe	908:930	The SZ composites maybe have potentials for applications as biomaterials.
25538055	4	6	theme	zein	606:609	arg1	domains					611:617	zein domains	606:617	zein domains	606:617	SEM and optical observation showed that starch and zein domains can be differed in the films and in a two phase separation status.
25538055	2	7	dep	microscope	336:345	arg1	testing					398:404	angle testing	392:404	angle testing	392:404	The structure and physical properties of the starch/zein (SZ) composite films were characterized by scanning electron microscope (SEM), optical microscopy and water contract angle testing.
25538055	2	8	theme	composite	280:288	arg1	films					290:294	the starch/zein (SZ) composite films	259:294	the starch/zein (SZ) composite films	259:294	The structure and physical properties of the starch/zein (SZ) composite films were characterized by scanning electron microscope (SEM), optical microscopy and water contract angle testing.
25538055	3	9	theme	recalcification	488:502	arg1	assay					520:524	plasma recalcification time, hemolysis assay	481:524	assay	520:524	The hemocompatibility and cytocompatibility of SZ films were evaluated by plasma recalcification time, hemolysis assay and cell culture experiment.
25538055	5	10	theme	films	752:756	arg1	hydrophilicity/hydrophobicity					715:743	the surface hydrophilicity/hydrophobicity	703:743	the surface hydrophilicity/hydrophobicity of the films	703:756	Glycerol affects the surface hydrophilicity/hydrophobicity of the films.
25538055	1	11	theme	films	121:125	arg1	series					101:106	A series	99:106	A series of composite films	99:125	A series of composite films were prepared from glycerol-plasticized starch and zein by intensive mixing and hot press.
25538055	3	12	theme	culture	535:541	arg1	experiment					543:552	cell culture experiment	530:552	cell culture experiment	530:552	The hemocompatibility and cytocompatibility of SZ films were evaluated by plasma recalcification time, hemolysis assay and cell culture experiment.
25538055	4	13	theme	phase	661:665	arg1	separation					667:676	a two phase separation	655:676	a two phase separation status	655:683	SEM and optical observation showed that starch and zein domains can be differed in the films and in a two phase separation status.
25538055	2	14	theme	angle	392:396	arg1	testing					398:404	angle testing	392:404	angle testing	392:404	The structure and physical properties of the starch/zein (SZ) composite films were characterized by scanning electron microscope (SEM), optical microscopy and water contract angle testing.
25538055	6	15	theme	SZ	826:827	arg1	composites					829:838	SZ composites	826:838	SZ composites	826:838	The hemocompatibility and cytocompatibility evaluation showed that SZ composites are anticoagulant materials with no hemolysis and low cytotoxicity.
25538055	6	15	theme	SZ	826:827	arg1	materials					858:866	anticoagulant materials	844:866	anticoagulant materials with no hemolysis and low cytotoxicity	844:905	The hemocompatibility and cytocompatibility evaluation showed that SZ composites are anticoagulant materials with no hemolysis and low cytotoxicity.
25538055	1	16	theme	intensive	186:194	arg1	mixing					196:201	intensive mixing	186:201	intensive mixing	186:201	A series of composite films were prepared from glycerol-plasticized starch and zein by intensive mixing and hot press.
25538055	3	17	theme	cell	530:533	arg1	experiment					543:552	cell culture experiment	530:552	cell culture experiment	530:552	The hemocompatibility and cytocompatibility of SZ films were evaluated by plasma recalcification time, hemolysis assay and cell culture experiment.
25538055	2	18	theme	scanning	318:325	arg1	SEM					348:350	SEM	348:350	SEM	348:350	The structure and physical properties of the starch/zein (SZ) composite films were characterized by scanning electron microscope (SEM), optical microscopy and water contract angle testing.
25538055	2	18	theme	scanning	318:325	arg1	microscope					336:345	scanning electron microscope	318:345	scanning electron microscope (SEM)	318:351	The structure and physical properties of the starch/zein (SZ) composite films were characterized by scanning electron microscope (SEM), optical microscopy and water contract angle testing.
25538055	2	19	theme	water	377:381	arg1	contract					383:390	water contract	377:390	water contract	377:390	The structure and physical properties of the starch/zein (SZ) composite films were characterized by scanning electron microscope (SEM), optical microscopy and water contract angle testing.
25538055	4	20	theme	optical	563:569	arg1	observation					571:581	optical observation	563:581	optical observation	563:581	SEM and optical observation showed that starch and zein domains can be differed in the films and in a two phase separation status.
25538055	3	21	dep	hemocompatibility	411:427	arg1	The					407:409	The	407:409	The	407:409	The hemocompatibility and cytocompatibility of SZ films were evaluated by plasma recalcification time, hemolysis assay and cell culture experiment.
25538055	3	22	dep	assay	520:524	arg1	hemolysis					510:518	plasma recalcification time, hemolysis assay	481:524	hemolysis	510:518	The hemocompatibility and cytocompatibility of SZ films were evaluated by plasma recalcification time, hemolysis assay and cell culture experiment.
25538055	6	23	with	materials	858:866	arg1	hemolysis					876:884	no hemolysis	873:884	no hemolysis	873:884	The hemocompatibility and cytocompatibility evaluation showed that SZ composites are anticoagulant materials with no hemolysis and low cytotoxicity.
25538055	6	23	with	materials	858:866	arg1	cytotoxicity					894:905	low cytotoxicity	890:905	low cytotoxicity	890:905	The hemocompatibility and cytocompatibility evaluation showed that SZ composites are anticoagulant materials with no hemolysis and low cytotoxicity.
25538055	1	24	theme	hot	207:209	arg1	press					211:215	hot press	207:215	hot press	207:215	A series of composite films were prepared from glycerol-plasticized starch and zein by intensive mixing and hot press.
25538055	0	25	theme	physical	11:18	arg1	properties					20:29	physical properties	11:29	physical properties	11:29	Structure, physical properties, hemocompatibility and cytocompatibility of starch/zein composites.
25538055	1	26	theme	composite	111:119	arg1	films					121:125	composite films	111:125	composite films	111:125	A series of composite films were prepared from glycerol-plasticized starch and zein by intensive mixing and hot press.
25538055	2	27	dep	structure	222:230	arg1	The					218:220	The	218:220	The	218:220	The structure and physical properties of the starch/zein (SZ) composite films were characterized by scanning electron microscope (SEM), optical microscopy and water contract angle testing.
25538055	5	28	theme	surface	707:713	arg1	hydrophilicity/hydrophobicity					715:743	the surface hydrophilicity/hydrophobicity	703:743	the surface hydrophilicity/hydrophobicity of the films	703:756	Glycerol affects the surface hydrophilicity/hydrophobicity of the films.
25538055	3	29	theme	films	457:461	arg1	cytocompatibility					433:449	cytocompatibility	433:449	cytocompatibility	433:449	The hemocompatibility and cytocompatibility of SZ films were evaluated by plasma recalcification time, hemolysis assay and cell culture experiment.
25538055	3	29	theme	films	457:461	arg1	hemocompatibility					411:427	hemocompatibility	411:427	hemocompatibility	411:427	The hemocompatibility and cytocompatibility of SZ films were evaluated by plasma recalcification time, hemolysis assay and cell culture experiment.
25538055	2	30	theme	physical	236:243	arg1	properties					245:254	physical properties	236:254	physical properties	236:254	The structure and physical properties of the starch/zein (SZ) composite films were characterized by scanning electron microscope (SEM), optical microscopy and water contract angle testing.
25538055	6	31	theme	cytocompatibility	785:801	arg1	evaluation					803:812	The hemocompatibility and cytocompatibility evaluation	759:812	The hemocompatibility and cytocompatibility evaluation	759:812	The hemocompatibility and cytocompatibility evaluation showed that SZ composites are anticoagulant materials with no hemolysis and low cytotoxicity.
25538055	6	32	theme	low	890:892	arg1	cytotoxicity					894:905	low cytotoxicity	890:905	low cytotoxicity	890:905	The hemocompatibility and cytocompatibility evaluation showed that SZ composites are anticoagulant materials with no hemolysis and low cytotoxicity.
25538055	3	33	theme	time	504:507	arg1	assay					520:524	plasma recalcification time, hemolysis assay	481:524	assay	520:524	The hemocompatibility and cytocompatibility of SZ films were evaluated by plasma recalcification time, hemolysis assay and cell culture experiment.
25538055	6	34	theme	hemocompatibility	763:779	arg1	evaluation					803:812	The hemocompatibility and cytocompatibility evaluation	759:812	The hemocompatibility and cytocompatibility evaluation	759:812	The hemocompatibility and cytocompatibility evaluation showed that SZ composites are anticoagulant materials with no hemolysis and low cytotoxicity.
25538055	4	35	theme	separation	667:676	arg1	status					678:683	a two phase separation status	655:683	a two phase separation status	655:683	SEM and optical observation showed that starch and zein domains can be differed in the films and in a two phase separation status.
25538055	3	36	theme	plasma	481:486	arg1	assay					520:524	plasma recalcification time, hemolysis assay	481:524	assay	520:524	The hemocompatibility and cytocompatibility of SZ films were evaluated by plasma recalcification time, hemolysis assay and cell culture experiment.
25538055	6	37	theme	no	873:874	arg1	hemolysis					876:884	no hemolysis	873:884	no hemolysis	873:884	The hemocompatibility and cytocompatibility evaluation showed that SZ composites are anticoagulant materials with no hemolysis and low cytotoxicity.
25538055	2	38	theme	starch/zein	263:273	arg1	films					290:294	the starch/zein (SZ) composite films	259:294	the starch/zein (SZ) composite films	259:294	The structure and physical properties of the starch/zein (SZ) composite films were characterized by scanning electron microscope (SEM), optical microscopy and water contract angle testing.
25538055	2	39	theme	electron	327:334	arg1	SEM					348:350	SEM	348:350	SEM	348:350	The structure and physical properties of the starch/zein (SZ) composite films were characterized by scanning electron microscope (SEM), optical microscopy and water contract angle testing.
25538055	2	39	theme	electron	327:334	arg1	microscope					336:345	scanning electron microscope	318:345	scanning electron microscope (SEM)	318:351	The structure and physical properties of the starch/zein (SZ) composite films were characterized by scanning electron microscope (SEM), optical microscopy and water contract angle testing.
25538055	0	40	theme	starch/zein	75:85	arg1	composites					87:96	starch/zein composites	75:96	starch/zein composites	75:96	Structure, physical properties, hemocompatibility and cytocompatibility of starch/zein composites.
25538055	1	41	theme	glycerol-plasticized	146:165	arg1	starch					167:172	glycerol-plasticized starch	146:172	glycerol-plasticized starch	146:172	A series of composite films were prepared from glycerol-plasticized starch and zein by intensive mixing and hot press.
25538055	3	42	theme	SZ	454:455	arg1	films					457:461	SZ films	454:461	SZ films	454:461	The hemocompatibility and cytocompatibility of SZ films were evaluated by plasma recalcification time, hemolysis assay and cell culture experiment.
25538055	2	43	theme	optical	354:360	arg1	microscopy					362:371	optical microscopy	354:371	optical microscopy	354:371	The structure and physical properties of the starch/zein (SZ) composite films were characterized by scanning electron microscope (SEM), optical microscopy and water contract angle testing.
26780124	5	0	theme	UV-vis	733:738	arg1	spectra					740:746	UV-vis spectra	733:746	UV-vis spectra	733:746	The composite particles containing Ag NPs were characterized using UV-vis spectra and scanning electron microscopy-energy dispersive X-ray spectrometry data.
26780124	6	1	theme	characteristic	828:841	arg1	peaks					843:847	The characteristic peaks	824:847	The characteristic peaks of Ag NPs in the UV-vis spectra and the element mapping or pattern	824:914	The characteristic peaks of Ag NPs in the UV-vis spectra and the element mapping or pattern revealed the formation of nanosized silver particles.
26780124	1	2	theme	numerous	181:188	arg1	applications					201:212	numerous biomedical applications	181:212	numerous biomedical applications	181:212	Silver nanoparticle (Ag NP)-loaded chitosan composites have numerous biomedical applications; however, fabricating uniform composite microparticles remains challenging.
26780124	7	3	theme	tests	999:1003	arg1	results					974:980	The results	970:980	The results of antibacterial tests	970:1003	The results of antibacterial tests indicated that both chitosan and composite particles showed antibacterial ability, and Ag NPs could enhance the inhibition rate and exhibited dose-dependent antibacterial ability.
26780124	5	4	theme	composite	670:678	arg1	particles					680:688	The composite particles	666:688	The composite particles containing Ag NPs	666:706	The composite particles containing Ag NPs were characterized using UV-vis spectra and scanning electron microscopy-energy dispersive X-ray spectrometry data.
26780124	2	5	theme	microparticles	403:416	arg1	synthesis					368:376	single-step and in situ synthesis	344:376	single-step and in situ synthesis of Ag NP-loaded chitosan microparticles	344:416	This paper presents a novel microfluidic approach for single-step and in situ synthesis of Ag NP-loaded chitosan microparticles.
26780124	4	6	theme	microfluidic	647:658	arg1	chip					660:663	the microfluidic chip	643:663	the microfluidic chip	643:663	In addition, the diameter of the composites can be tuned by adjusting the flow on the microfluidic chip.
26780124	4	7	theme	composites	594:603	arg1	diameter					578:585	the diameter	574:585	the diameter of the composites	574:603	In addition, the diameter of the composites can be tuned by adjusting the flow on the microfluidic chip.
26780124	3	8	theme	uniform	460:466	arg1	microparticles					507:520	uniform and monodisperse Ag NP-loaded chitosan microparticles	460:520	uniform and monodisperse Ag NP-loaded chitosan microparticles measuring several hundred micrometers	460:558	This proposed approach enables obtaining uniform and monodisperse Ag NP-loaded chitosan microparticles measuring several hundred micrometers.
26780124	2	9	theme	single-step	344:354	arg1	synthesis					368:376	single-step and in situ synthesis	344:376	single-step and in situ synthesis of Ag NP-loaded chitosan microparticles	344:416	This paper presents a novel microfluidic approach for single-step and in situ synthesis of Ag NP-loaded chitosan microparticles.
26780124	8	10	theme	chitosan	1225:1232	arg1	properties					1200:1209	the properties	1196:1209	the properties of Ag NPs and chitosan	1196:1232	Because of the properties of Ag NPs and chitosan, the synthesized composite microparticles can be used in several future potential applications, such as bactericidal agents for water disinfection, antipathogens, and surface plasma resonance enhancers.
26780124	3	11	theme	proposed	424:431	arg1	approach					433:440	This proposed approach	419:440	This proposed approach	419:440	This proposed approach enables obtaining uniform and monodisperse Ag NP-loaded chitosan microparticles measuring several hundred micrometers.
26780124	5	12	theme	scanning	752:759	arg1	data					818:821	scanning electron microscopy-energy dispersive X-ray spectrometry data	752:821	scanning electron microscopy-energy dispersive X-ray spectrometry data	752:821	The composite particles containing Ag NPs were characterized using UV-vis spectra and scanning electron microscopy-energy dispersive X-ray spectrometry data.
26780124	7	13	theme	antibacterial	1065:1077	arg1	ability					1079:1085	antibacterial ability	1065:1085	antibacterial ability	1065:1085	The results of antibacterial tests indicated that both chitosan and composite particles showed antibacterial ability, and Ag NPs could enhance the inhibition rate and exhibited dose-dependent antibacterial ability.
26780124	8	14	theme	several	1291:1297	arg1	applications					1316:1327	several future potential applications	1291:1327	several future potential applications	1291:1327	Because of the properties of Ag NPs and chitosan, the synthesized composite microparticles can be used in several future potential applications, such as bactericidal agents for water disinfection, antipathogens, and surface plasma resonance enhancers.
26780124	8	14	theme	several	1291:1297	arg1	enhancers					1426:1434	surface plasma resonance enhancers	1401:1434	surface plasma resonance enhancers	1401:1434	Because of the properties of Ag NPs and chitosan, the synthesized composite microparticles can be used in several future potential applications, such as bactericidal agents for water disinfection, antipathogens, and surface plasma resonance enhancers.
26780124	8	14	theme	several	1291:1297	arg1	antipathogens					1382:1394	antipathogens	1382:1394	antipathogens	1382:1394	Because of the properties of Ag NPs and chitosan, the synthesized composite microparticles can be used in several future potential applications, such as bactericidal agents for water disinfection, antipathogens, and surface plasma resonance enhancers.
26780124	8	14	theme	several	1291:1297	arg1	agents					1351:1356	bactericidal agents	1338:1356	bactericidal agents for water disinfection	1338:1379	Because of the properties of Ag NPs and chitosan, the synthesized composite microparticles can be used in several future potential applications, such as bactericidal agents for water disinfection, antipathogens, and surface plasma resonance enhancers.
26780124	2	15	dep	in	360:361	arg1	situ					363:366	situ	363:366	situ	363:366	This paper presents a novel microfluidic approach for single-step and in situ synthesis of Ag NP-loaded chitosan microparticles.
26780124	5	16	theme	Ag	701:702	arg1	NPs					704:706	Ag NPs	701:706	Ag NPs	701:706	The composite particles containing Ag NPs were characterized using UV-vis spectra and scanning electron microscopy-energy dispersive X-ray spectrometry data.
26780124	8	17	theme	water	1362:1366	arg1	disinfection					1368:1379	water disinfection	1362:1379	water disinfection	1362:1379	Because of the properties of Ag NPs and chitosan, the synthesized composite microparticles can be used in several future potential applications, such as bactericidal agents for water disinfection, antipathogens, and surface plasma resonance enhancers.
26780124	3	18	theme	monodisperse	472:483	arg1	microparticles					507:520	uniform and monodisperse Ag NP-loaded chitosan microparticles	460:520	uniform and monodisperse Ag NP-loaded chitosan microparticles measuring several hundred micrometers	460:558	This proposed approach enables obtaining uniform and monodisperse Ag NP-loaded chitosan microparticles measuring several hundred micrometers.
26780124	1	19	contain	have	176:179	arg2	applications					201:212	numerous biomedical applications	181:212	numerous biomedical applications	181:212	Silver nanoparticle (Ag NP)-loaded chitosan composites have numerous biomedical applications; however, fabricating uniform composite microparticles remains challenging.
26780124	1	19	contain	have	176:179	arg1	composites					165:174	Silver nanoparticle (Ag NP)-loaded chitosan composites	121:174	Silver nanoparticle (Ag NP)-loaded chitosan composites	121:174	Silver nanoparticle (Ag NP)-loaded chitosan composites have numerous biomedical applications; however, fabricating uniform composite microparticles remains challenging.
26780124	3	20	theme	Ag	485:486	arg1	microparticles					507:520	uniform and monodisperse Ag NP-loaded chitosan microparticles	460:520	uniform and monodisperse Ag NP-loaded chitosan microparticles measuring several hundred micrometers	460:558	This proposed approach enables obtaining uniform and monodisperse Ag NP-loaded chitosan microparticles measuring several hundred micrometers.
26780124	6	21	theme	element	889:895	arg1	mapping					897:903	element mapping	889:903	element mapping	889:903	The characteristic peaks of Ag NPs in the UV-vis spectra and the element mapping or pattern revealed the formation of nanosized silver particles.
26780124	2	22	theme	microfluidic	318:329	arg1	approach					331:338	a novel microfluidic approach	310:338	a novel microfluidic approach for single-step and in situ synthesis of Ag NP-loaded chitosan microparticles	310:416	This paper presents a novel microfluidic approach for single-step and in situ synthesis of Ag NP-loaded chitosan microparticles.
26780124	3	23	theme	several	532:538	arg1	micrometers					548:558	hundred micrometers	540:558	several hundred micrometers	532:558	This proposed approach enables obtaining uniform and monodisperse Ag NP-loaded chitosan microparticles measuring several hundred micrometers.
26780124	0	24	theme	assisted	13:20	arg1	synthesis					22:30	Microfluidic assisted synthesis	0:30	Microfluidic assisted synthesis of silver nanoparticle-chitosan composite	0:72	Microfluidic assisted synthesis of silver nanoparticle-chitosan composite microparticles for antibacterial applications.
26780124	8	25	theme	composite	1251:1259	arg1	microparticles					1261:1274	the synthesized composite microparticles	1235:1274	the synthesized composite microparticles	1235:1274	Because of the properties of Ag NPs and chitosan, the synthesized composite microparticles can be used in several future potential applications, such as bactericidal agents for water disinfection, antipathogens, and surface plasma resonance enhancers.
26780124	1	26	theme	Silver	121:126	arg1	nanoparticle					128:139	Silver nanoparticle	121:139	Silver nanoparticle (Ag NP)-loaded chitosan composites	121:174	Silver nanoparticle (Ag NP)-loaded chitosan composites have numerous biomedical applications; however, fabricating uniform composite microparticles remains challenging.
26780124	1	26	theme	Silver	121:126	arg1	Ag					142:143	Ag NP	142:146	Ag NP	142:146	Silver nanoparticle (Ag NP)-loaded chitosan composites have numerous biomedical applications; however, fabricating uniform composite microparticles remains challenging.
26780124	3	27	theme	chitosan	498:505	arg1	microparticles					507:520	uniform and monodisperse Ag NP-loaded chitosan microparticles	460:520	uniform and monodisperse Ag NP-loaded chitosan microparticles measuring several hundred micrometers	460:558	This proposed approach enables obtaining uniform and monodisperse Ag NP-loaded chitosan microparticles measuring several hundred micrometers.
26780124	0	28	theme	Microfluidic	0:11	arg1	synthesis					22:30	Microfluidic assisted synthesis	0:30	Microfluidic assisted synthesis of silver nanoparticle-chitosan composite	0:72	Microfluidic assisted synthesis of silver nanoparticle-chitosan composite microparticles for antibacterial applications.
26780124	8	29	used	used	1283:1286	arg2	microparticles					1261:1274	the synthesized composite microparticles	1235:1274	the synthesized composite microparticles	1235:1274	Because of the properties of Ag NPs and chitosan, the synthesized composite microparticles can be used in several future potential applications, such as bactericidal agents for water disinfection, antipathogens, and surface plasma resonance enhancers.
26780124	1	30	theme	nanoparticle	128:139	arg1	composites					165:174	Silver nanoparticle (Ag NP)-loaded chitosan composites	121:174	Silver nanoparticle (Ag NP)-loaded chitosan composites	121:174	Silver nanoparticle (Ag NP)-loaded chitosan composites have numerous biomedical applications; however, fabricating uniform composite microparticles remains challenging.
26780124	4	31	from	flow	635:638	arg1	chip					660:663	the microfluidic chip	643:663	the microfluidic chip	643:663	In addition, the diameter of the composites can be tuned by adjusting the flow on the microfluidic chip.
26780124	8	32	theme	Ag	1214:1215	arg1	NPs					1217:1219	Ag NPs	1214:1219	Ag NPs	1214:1219	Because of the properties of Ag NPs and chitosan, the synthesized composite microparticles can be used in several future potential applications, such as bactericidal agents for water disinfection, antipathogens, and surface plasma resonance enhancers.
26780124	5	33	theme	spectrometry	805:816	arg1	data					818:821	scanning electron microscopy-energy dispersive X-ray spectrometry data	752:821	scanning electron microscopy-energy dispersive X-ray spectrometry data	752:821	The composite particles containing Ag NPs were characterized using UV-vis spectra and scanning electron microscopy-energy dispersive X-ray spectrometry data.
26780124	7	34	dep	chitosan	1025:1032	arg1	particles					1048:1056	particles	1048:1056	particles	1048:1056	The results of antibacterial tests indicated that both chitosan and composite particles showed antibacterial ability, and Ag NPs could enhance the inhibition rate and exhibited dose-dependent antibacterial ability.
26780124	2	35	theme	in	360:361	arg1	synthesis					368:376	single-step and in situ synthesis	344:376	single-step and in situ synthesis of Ag NP-loaded chitosan microparticles	344:416	This paper presents a novel microfluidic approach for single-step and in situ synthesis of Ag NP-loaded chitosan microparticles.
26780124	6	36	dep	mapping	897:903	arg1	the					885:887	the	885:887	the	885:887	The characteristic peaks of Ag NPs in the UV-vis spectra and the element mapping or pattern revealed the formation of nanosized silver particles.
26780124	1	37	theme	fabricating	224:234	arg1	microparticles					254:267	fabricating uniform composite microparticles	224:267	fabricating uniform composite microparticles	224:267	Silver nanoparticle (Ag NP)-loaded chitosan composites have numerous biomedical applications; however, fabricating uniform composite microparticles remains challenging.
26780124	1	38	theme	biomedical	190:199	arg1	applications					201:212	numerous biomedical applications	181:212	numerous biomedical applications	181:212	Silver nanoparticle (Ag NP)-loaded chitosan composites have numerous biomedical applications; however, fabricating uniform composite microparticles remains challenging.
26780124	1	39	theme	uniform	236:242	arg1	microparticles					254:267	fabricating uniform composite microparticles	224:267	fabricating uniform composite microparticles	224:267	Silver nanoparticle (Ag NP)-loaded chitosan composites have numerous biomedical applications; however, fabricating uniform composite microparticles remains challenging.
26780124	0	40	theme	nanoparticle-chitosan	42:62	arg1	composite					64:72	silver nanoparticle-chitosan composite	35:72	silver nanoparticle-chitosan composite	35:72	Microfluidic assisted synthesis of silver nanoparticle-chitosan composite microparticles for antibacterial applications.
26780124	8	41	theme	plasma	1409:1414	arg1	resonance					1416:1424	surface plasma resonance	1401:1424	surface plasma resonance enhancers	1401:1434	Because of the properties of Ag NPs and chitosan, the synthesized composite microparticles can be used in several future potential applications, such as bactericidal agents for water disinfection, antipathogens, and surface plasma resonance enhancers.
26780124	8	42	theme	NPs	1217:1219	arg1	properties					1200:1209	the properties	1196:1209	the properties of Ag NPs and chitosan	1196:1232	Because of the properties of Ag NPs and chitosan, the synthesized composite microparticles can be used in several future potential applications, such as bactericidal agents for water disinfection, antipathogens, and surface plasma resonance enhancers.
26780124	8	43	theme	surface	1401:1407	arg1	resonance					1416:1424	surface plasma resonance	1401:1424	surface plasma resonance enhancers	1401:1434	Because of the properties of Ag NPs and chitosan, the synthesized composite microparticles can be used in several future potential applications, such as bactericidal agents for water disinfection, antipathogens, and surface plasma resonance enhancers.
26780124	1	44	theme	composite	244:252	arg1	microparticles					254:267	fabricating uniform composite microparticles	224:267	fabricating uniform composite microparticles	224:267	Silver nanoparticle (Ag NP)-loaded chitosan composites have numerous biomedical applications; however, fabricating uniform composite microparticles remains challenging.
26780124	0	45	theme	silver	35:40	arg1	composite					64:72	silver nanoparticle-chitosan composite	35:72	silver nanoparticle-chitosan composite	35:72	Microfluidic assisted synthesis of silver nanoparticle-chitosan composite microparticles for antibacterial applications.
26780124	3	46	theme	NP-loaded	488:496	arg1	microparticles					507:520	uniform and monodisperse Ag NP-loaded chitosan microparticles	460:520	uniform and monodisperse Ag NP-loaded chitosan microparticles measuring several hundred micrometers	460:558	This proposed approach enables obtaining uniform and monodisperse Ag NP-loaded chitosan microparticles measuring several hundred micrometers.
26780124	6	47	theme	particles	959:967	arg1	formation					929:937	the formation	925:937	the formation of nanosized silver particles	925:967	The characteristic peaks of Ag NPs in the UV-vis spectra and the element mapping or pattern revealed the formation of nanosized silver particles.
26780124	7	48	theme	dose-dependent	1147:1160	arg1	ability					1176:1182	dose-dependent antibacterial ability	1147:1182	dose-dependent antibacterial ability	1147:1182	The results of antibacterial tests indicated that both chitosan and composite particles showed antibacterial ability, and Ag NPs could enhance the inhibition rate and exhibited dose-dependent antibacterial ability.
26780124	6	49	theme	UV-vis	866:871	arg1	spectra					873:879	the UV-vis spectra	862:879	the UV-vis spectra	862:879	The characteristic peaks of Ag NPs in the UV-vis spectra and the element mapping or pattern revealed the formation of nanosized silver particles.
26780124	8	50	theme	resonance	1416:1424	arg1	enhancers					1426:1434	surface plasma resonance enhancers	1401:1434	surface plasma resonance enhancers	1401:1434	Because of the properties of Ag NPs and chitosan, the synthesized composite microparticles can be used in several future potential applications, such as bactericidal agents for water disinfection, antipathogens, and surface plasma resonance enhancers.
26780124	5	51	theme	electron	761:768	arg1	data					818:821	scanning electron microscopy-energy dispersive X-ray spectrometry data	752:821	scanning electron microscopy-energy dispersive X-ray spectrometry data	752:821	The composite particles containing Ag NPs were characterized using UV-vis spectra and scanning electron microscopy-energy dispersive X-ray spectrometry data.
26780124	6	52	theme	silver	952:957	arg1	particles					959:967	nanosized silver particles	942:967	nanosized silver particles	942:967	The characteristic peaks of Ag NPs in the UV-vis spectra and the element mapping or pattern revealed the formation of nanosized silver particles.
26780124	2	53	theme	chitosan	394:401	arg1	microparticles					403:416	Ag NP-loaded chitosan microparticles	381:416	Ag NP-loaded chitosan microparticles	381:416	This paper presents a novel microfluidic approach for single-step and in situ synthesis of Ag NP-loaded chitosan microparticles.
26780124	0	54	theme	composite	64:72	arg1	synthesis					22:30	Microfluidic assisted synthesis	0:30	Microfluidic assisted synthesis of silver nanoparticle-chitosan composite	0:72	Microfluidic assisted synthesis of silver nanoparticle-chitosan composite microparticles for antibacterial applications.
26780124	5	55	theme	microscopy-energy	770:786	arg1	data					818:821	scanning electron microscopy-energy dispersive X-ray spectrometry data	752:821	scanning electron microscopy-energy dispersive X-ray spectrometry data	752:821	The composite particles containing Ag NPs were characterized using UV-vis spectra and scanning electron microscopy-energy dispersive X-ray spectrometry data.
26780124	6	56	theme	nanosized	942:950	arg1	particles					959:967	nanosized silver particles	942:967	nanosized silver particles	942:967	The characteristic peaks of Ag NPs in the UV-vis spectra and the element mapping or pattern revealed the formation of nanosized silver particles.
26780124	2	57	theme	NP-loaded	384:392	arg1	microparticles					403:416	Ag NP-loaded chitosan microparticles	381:416	Ag NP-loaded chitosan microparticles	381:416	This paper presents a novel microfluidic approach for single-step and in situ synthesis of Ag NP-loaded chitosan microparticles.
26780124	5	58	theme	dispersive	788:797	arg1	data					818:821	scanning electron microscopy-energy dispersive X-ray spectrometry data	752:821	scanning electron microscopy-energy dispersive X-ray spectrometry data	752:821	The composite particles containing Ag NPs were characterized using UV-vis spectra and scanning electron microscopy-energy dispersive X-ray spectrometry data.
26780124	2	59	theme	novel	312:316	arg1	approach					331:338	a novel microfluidic approach	310:338	a novel microfluidic approach for single-step and in situ synthesis of Ag NP-loaded chitosan microparticles	310:416	This paper presents a novel microfluidic approach for single-step and in situ synthesis of Ag NP-loaded chitosan microparticles.
26780124	6	60	theme	NPs	855:857	arg1	peaks					843:847	The characteristic peaks	824:847	The characteristic peaks of Ag NPs in the UV-vis spectra and the element mapping or pattern	824:914	The characteristic peaks of Ag NPs in the UV-vis spectra and the element mapping or pattern revealed the formation of nanosized silver particles.
26780124	1	61	theme	-loaded	148:154	arg1	composites					165:174	Silver nanoparticle (Ag NP)-loaded chitosan composites	121:174	Silver nanoparticle (Ag NP)-loaded chitosan composites	121:174	Silver nanoparticle (Ag NP)-loaded chitosan composites have numerous biomedical applications; however, fabricating uniform composite microparticles remains challenging.
26780124	5	62	theme	X-ray	799:803	arg1	data					818:821	scanning electron microscopy-energy dispersive X-ray spectrometry data	752:821	scanning electron microscopy-energy dispersive X-ray spectrometry data	752:821	The composite particles containing Ag NPs were characterized using UV-vis spectra and scanning electron microscopy-energy dispersive X-ray spectrometry data.
26780124	8	63	theme	bactericidal	1338:1349	arg1	agents					1351:1356	bactericidal agents	1338:1356	bactericidal agents for water disinfection	1338:1379	Because of the properties of Ag NPs and chitosan, the synthesized composite microparticles can be used in several future potential applications, such as bactericidal agents for water disinfection, antipathogens, and surface plasma resonance enhancers.
26780124	8	64	theme	future	1299:1304	arg1	applications					1316:1327	several future potential applications	1291:1327	several future potential applications	1291:1327	Because of the properties of Ag NPs and chitosan, the synthesized composite microparticles can be used in several future potential applications, such as bactericidal agents for water disinfection, antipathogens, and surface plasma resonance enhancers.
26780124	8	64	theme	future	1299:1304	arg1	enhancers					1426:1434	surface plasma resonance enhancers	1401:1434	surface plasma resonance enhancers	1401:1434	Because of the properties of Ag NPs and chitosan, the synthesized composite microparticles can be used in several future potential applications, such as bactericidal agents for water disinfection, antipathogens, and surface plasma resonance enhancers.
26780124	8	64	theme	future	1299:1304	arg1	antipathogens					1382:1394	antipathogens	1382:1394	antipathogens	1382:1394	Because of the properties of Ag NPs and chitosan, the synthesized composite microparticles can be used in several future potential applications, such as bactericidal agents for water disinfection, antipathogens, and surface plasma resonance enhancers.
26780124	8	64	theme	future	1299:1304	arg1	agents					1351:1356	bactericidal agents	1338:1356	bactericidal agents for water disinfection	1338:1379	Because of the properties of Ag NPs and chitosan, the synthesized composite microparticles can be used in several future potential applications, such as bactericidal agents for water disinfection, antipathogens, and surface plasma resonance enhancers.
26780124	6	65	theme	Ag	852:853	arg1	NPs					855:857	Ag NPs	852:857	Ag NPs	852:857	The characteristic peaks of Ag NPs in the UV-vis spectra and the element mapping or pattern revealed the formation of nanosized silver particles.
26780124	1	66	theme	chitosan	156:163	arg1	composites					165:174	Silver nanoparticle (Ag NP)-loaded chitosan composites	121:174	Silver nanoparticle (Ag NP)-loaded chitosan composites	121:174	Silver nanoparticle (Ag NP)-loaded chitosan composites have numerous biomedical applications; however, fabricating uniform composite microparticles remains challenging.
26780124	2	67	theme	Ag	381:382	arg1	microparticles					403:416	Ag NP-loaded chitosan microparticles	381:416	Ag NP-loaded chitosan microparticles	381:416	This paper presents a novel microfluidic approach for single-step and in situ synthesis of Ag NP-loaded chitosan microparticles.
26780124	0	68	theme	antibacterial	93:105	arg1	applications					107:118	antibacterial applications	93:118	antibacterial applications	93:118	Microfluidic assisted synthesis of silver nanoparticle-chitosan composite microparticles for antibacterial applications.
26780124	7	69	theme	inhibition	1117:1126	arg1	rate					1128:1131	the inhibition rate	1113:1131	the inhibition rate	1113:1131	The results of antibacterial tests indicated that both chitosan and composite particles showed antibacterial ability, and Ag NPs could enhance the inhibition rate and exhibited dose-dependent antibacterial ability.
26780124	7	70	theme	antibacterial	1162:1174	arg1	ability					1176:1182	dose-dependent antibacterial ability	1147:1182	dose-dependent antibacterial ability	1147:1182	The results of antibacterial tests indicated that both chitosan and composite particles showed antibacterial ability, and Ag NPs could enhance the inhibition rate and exhibited dose-dependent antibacterial ability.
26780124	6	71	from	peaks	843:847	arg1	spectra					873:879	the UV-vis spectra	862:879	the UV-vis spectra	862:879	The characteristic peaks of Ag NPs in the UV-vis spectra and the element mapping or pattern revealed the formation of nanosized silver particles.
26780124	6	71	from	peaks	843:847	arg1	mapping					897:903	element mapping	889:903	element mapping	889:903	The characteristic peaks of Ag NPs in the UV-vis spectra and the element mapping or pattern revealed the formation of nanosized silver particles.
26780124	5	72	contain	containing	690:699	arg1	particles					680:688	The composite particles	666:688	The composite particles containing Ag NPs	666:706	The composite particles containing Ag NPs were characterized using UV-vis spectra and scanning electron microscopy-energy dispersive X-ray spectrometry data.
26780124	5	72	contain	containing	690:699	arg2	NPs					704:706	Ag NPs	701:706	Ag NPs	701:706	The composite particles containing Ag NPs were characterized using UV-vis spectra and scanning electron microscopy-energy dispersive X-ray spectrometry data.
26780124	8	73	theme	synthesized	1239:1249	arg1	microparticles					1261:1274	the synthesized composite microparticles	1235:1274	the synthesized composite microparticles	1235:1274	Because of the properties of Ag NPs and chitosan, the synthesized composite microparticles can be used in several future potential applications, such as bactericidal agents for water disinfection, antipathogens, and surface plasma resonance enhancers.
26780124	7	74	theme	Ag	1092:1093	arg1	NPs					1095:1097	Ag NPs	1092:1097	Ag NPs	1092:1097	The results of antibacterial tests indicated that both chitosan and composite particles showed antibacterial ability, and Ag NPs could enhance the inhibition rate and exhibited dose-dependent antibacterial ability.
26780124	8	75	theme	potential	1306:1314	arg1	applications					1316:1327	several future potential applications	1291:1327	several future potential applications	1291:1327	Because of the properties of Ag NPs and chitosan, the synthesized composite microparticles can be used in several future potential applications, such as bactericidal agents for water disinfection, antipathogens, and surface plasma resonance enhancers.
26780124	8	75	theme	potential	1306:1314	arg1	enhancers					1426:1434	surface plasma resonance enhancers	1401:1434	surface plasma resonance enhancers	1401:1434	Because of the properties of Ag NPs and chitosan, the synthesized composite microparticles can be used in several future potential applications, such as bactericidal agents for water disinfection, antipathogens, and surface plasma resonance enhancers.
26780124	8	75	theme	potential	1306:1314	arg1	antipathogens					1382:1394	antipathogens	1382:1394	antipathogens	1382:1394	Because of the properties of Ag NPs and chitosan, the synthesized composite microparticles can be used in several future potential applications, such as bactericidal agents for water disinfection, antipathogens, and surface plasma resonance enhancers.
26780124	8	75	theme	potential	1306:1314	arg1	agents					1351:1356	bactericidal agents	1338:1356	bactericidal agents for water disinfection	1338:1379	Because of the properties of Ag NPs and chitosan, the synthesized composite microparticles can be used in several future potential applications, such as bactericidal agents for water disinfection, antipathogens, and surface plasma resonance enhancers.
26780124	7	76	theme	antibacterial	985:997	arg1	tests					999:1003	antibacterial tests	985:1003	antibacterial tests	985:1003	The results of antibacterial tests indicated that both chitosan and composite particles showed antibacterial ability, and Ag NPs could enhance the inhibition rate and exhibited dose-dependent antibacterial ability.